bluuebunny/crossref_metadata_2025_split huggingface dataset:
Structure and histochemistry of the stem of Dracaena cambodiana Pierre ex Gagnep
Authors: Zhang Yuxiu , Liu Yang , Wang Bocheng , Fu Meilong , Liu Peiwei , Wei Jian‐he 
Published: March 2023

AbstractDracaena cambodiana Pierre ex Gagnep is an important plant resource for producing dragon's blood and one of most popular ornamental trees in China. For a better understanding of the physiological function of the stem, the structural characteristics and main substance histological location of the stems of D. cambodiana were studied. The structural characteristics of the different developmental stages of stems of D. cambodiana were observed and described detailly. And then a schematic diagram of the mature stem was created. Histochemical staining showed that two kinds of polysaccharides distributed in parenchymal cells. Saponins distributed mainly in ground tissue and phenolic compounds distributed mainly in the thick cell walls. An abundant of calcium oxalate raphide bundles were identified in cortex and primary tissue. Finally, the role of the above results in the taxonomy of Dracaena species and in their strong adaptability was discussed.
Neurosensory anatomy and function in Seymouria
Authors: Bazzana‐Adams Kayla D. , Evans David C. , Bevitt Joseph J. , Reisz Robert R. 
Published: March 2023

AbstractSeymouria is among the best‐known stem amniotes and holds an important phylogenetic position for discussions of amniote evolution. Previous work has focused primarily on the osteology of Seymouria, with recent interest turning to the application of computed tomography (CT) to study the internal features. We utilized neutron CT to reconstruct the first virtual cranial endocast and the first complete otic endocasts of Seymouria, revealing previously unrecognized details of its palaeoneuroanatomy. The brain and inner ear of Seymouria are largely plesiomorphic relative to later‐diverging crown amniotes, showing no indication of increased encephalization or braincase ossification. Our results also clarify the plesiomorphic condition for carotid artery morphology in amniotes, with Seymouria showing a similar condition to basal members of both the synapsid and sauropsid lineages. The reconstructed neuroanatomy also indicates that Seymouria did not possess any particular neuroanatomical specializations, despite the probable presence of an impedance matching hearing system.
The development of a Trauma Resiliency Scale (tRS‐18)
Authors: Sinkler Margaret A. , Alejo Andrew , Agel Julie , Hendrickson Sarah , Breslin Mary A. , Vallier Heather A. 
Published: March 2023

AbstractResiliency consists of three core components, which include presence of adversity, protective factors to overcome adversity, and positive outcomes or growth. Therefore, resiliency aligns with the trauma recovery process. This paper describes development of the Trauma Resiliency Scale (tRS) to quantify the resiliency of trauma patients upon presentation and during recovery. Scale items were proposed and reviewed by an expert panel. Group construct validity testing was performed using both individual and focus group feedback with item analysis. Reliability was measured with test–retest administered 14 days apart and evaluated with intraclass correlation coefficient. One hundred and twenty‐three items were initially proposed. Following item categorizing, a preliminary 17‐item questionnaire was created. The questionnaire was administered to 40 individual participants and a trauma survivor focus group to evaluate construct validity. Following group construct testing, an 18‐item Trauma Resiliency Scale (tRS‐18) was proposed. Twenty‐four participants were given the tRS‐18 twice, 14 days apart to establish test–retest. Sixteen of the 18 questions had an intraclass correlation >0.7 (0.793–0.949). The remaining two questions underperformed based on the ICC (0.592 and 0.493) and were manually evaluated for inclusion. The final tRS‐18 is a brief, self‐administered measure of resiliency designed specifically for trauma patients. Sound psychometric properties including face validity, construct validity, and reliability of the instrument have been demonstrated. The tRS‐18 may quantify resiliency at any time point with potential to be predictive of progress during recovery. Level of Evidence III, prognostic.
Association between plasma trans fatty acids and chronic periodontitis: Results from a nationally representative cross‐sectional survey
Authors: Wu Yuying , Qiu Yu , Wu Yuxuan , Deng Qingrong , Yu Yiming , Yang Han , Chen Qiansi , He Baochang , Yan Fuhua , Li Yanfen , Chen Fa 
Published: March 2023

AbstractBackgroundTrans fatty acid (TFA) consumption has been reported to harbor proinflammatory characteristics and increasing oxidative stress properties, but there has been little research into its association with periodontitis. This study aimed to explore the potential association between TFAs and periodontitis.MethodsThis large population‐based study included participants from the National Health and Nutrition Examination Survey (2009‒2010). Weighted binary and ordinal logistic regressions were used to calculate the odds ratios (ORs) and 95% confidence intervals (CIs) to evaluate the relationship between plasma TFAs and periodontitis.ResultsA total of 1433 eligible participants, 793 (55.3%) participants with periodontitis and 640 (44.7%) without periodontitis were included. Univariate logistic regression revealed significant associations between plasma trans‐11‐octadecenoic acid, trans‐9‐octadecenoic acid, the sum of trans‐octadecenoic acids, and the sum of TFAs and periodontitis (all P < 0.01). After controlling for the potential confounders, these four types of TFAs remained significantly associated with periodontitis (the ORs and 95% CIs per interquartile range increase were 1.16 (1.01–1.33), 1.20 (1.03–1.39), 1.18 (1.02–1.35), and 1.17 (1.01–1.35), respectively). Notably, these positive associations were more pronounced among overweight/obese populations. Additionally, plasma trans‐9‐octadecenoic acid levels were found to be associated with periodontitis severity.ConclusionsThis study suggests a significant positive association between certain plasma TFAs and chronic periodontitis, especially among overweight/obese populations. These findings provide new insights into periodontitis prevention from a dietary perspective.
Bioactive compounds, functional properties, and technological application of Passiflora quadrangularis: A review
Authors: Cruz Josias M. A. , Pereira Zilanir C. , Corrêa Renilto F. , Lamarão Carlos V. , Sanches Edgar A. , Campelo Pedro H. , Bezerra Jaqueline de A. 
Published: March 2023

AbstractThe objective of this research was to evaluate the bioactive compounds and functional properties of Passiflora quadrangularis, aiming to contribute to future studies on the elaboration of functional products based on this species. The chemical analysis of flavonoids from the leaves, as well as the chemical composition of fruits (specifically the pericarp) were accessed herein. The functional properties are directly dependent on specific extracts, and the aqueous extract and the flavonoid fraction are responsible for the sedative property. The presence of C‐glycosyl flavonoids is highly reported in the chemical composition, highlighting the vitexin‐2″‐O‐xyloside. Few studies have been focused on this species, and most of them are devoted to the sedative properties of the genus Passiflora spp. However, a review on the main available information on this species is of extreme interest for the research and development of products in pharmaceuticals, cosmetics, and food areas.
Enzyme‐assisted HPTLC method for the simultaneous analysis of inositol phosphates and phosphate
Authors: Henninger Corinna , Spangenberg Bernd , Schmidt Mario , Ochsenreither Katrin , Eisele Thomas 
Published: March 2023

AbstractBackgroundThe analysis of myo‐inositol phosphates (InsPx) released by phytases during phytic acid degradation is challenging and time‐consuming, particularly in terms of sample preparation, isomer separation, and detection. However, a fast and robust analysis method is crucial when screening for phytases during protein engineering approaches, which result in a large number of samples, to ensure reliable identification of promising novel enzymes or target variants with improved characteristics, for example, pH range, thermal stability, and phosphate release kinetics.ResultsThe simultaneous analysis of several InsPx (InsP1‐InsP4 and InsP5 + 6) as well as free phosphate was established on cellulose HPTLC plates using a buffered mobile phase. Inositol phosphates were subsequently stained using a novel enzyme‐assisted staining procedure. Immobilized InsPx were hydrolyzed by a phytase solution of Quantum® Blueliquid 5G followed by a molybdate reagent derivatization. Resulting blue zones were captured by DAD scan. The method shows good repeatability (intra‐day and intra‐lab) with maximum deviations of the Rf value of 0.01. The HPTLC method was applied to three commercially available phytases at two pH levels relevant to the gastrointestinal tract of poultry (pH 5.5 and pH 3.6) to observe their phytate degradation pattern and thus visualize their InsPx fingerprint.ConclusionThis HPTLC method presents a semi‐high‐throughput analysis for the simultaneous analysis of phytic acid and the resulting lower inositol phosphates after its enzymatic hydrolysis and is also an effective tool to visualize the InsPx fingerprints and possible accumulations of inositol phosphates.
Does biochar addition improve soil physicochemical properties, bacterial community and alfalfa growth for saline soils?
Authors: Chen Yang , Qiu Yuan , Hao Xinmei , Tong Ling , Li Sien , Kang Shaozhong 
Published: March 2023

AbstractBiochar addition has been proven to have great potential for improving soil physicochemical properties and crop growth in salt‐affected soils. However, whether adding biochar could affect alfalfa growth and soil bacterial community in salt‐affected soils of arid regions remains poorly understood. A pot experiment was carried out to investigate the effects of corn straw biochar addition on soil physicochemical properties, bacterial community and alfalfa yield in saline soils. There were 12 treatments in total, combining three biochar addition rates of 0, 20 and 40 g kg−1 (referred to as B0, B2 and B4, respectively) and four salinity levels of 0, 2, 4 and 6 g kg−1 (referred as to S0, S2, S4 and S6, respectively). The results indicated that biochar addition significantly increased soil organic carbon (SOC), electrical conductivity (EC), soil water content (SWC), available nitrogen (AN) and phosphorus (AP) and mesopores fraction, but decreased soil bulk density (BD), pH and macropores fraction for different salinity levels. In general, biochar addition had an adverse effect on alfalfa yield due to the aggregated stresses from the increased EC and mesopores fraction, especially at high salinity levels. On average, biochar addition decreased the total yield of the two years by 14.2%, 25.3%, 38.3% and 60.4% for S0, S2, S4 and S6, respectively. Biochar addition significantly changed bacterial community composition with higher relative abundances of Proteobacteria and Bacteroidetes but lower relative abundances of Actinobacteria and Acidobacteria under S2–S6. On average, both soil salinity and biochar treatments decreased soil bacterial richness by 5.7–14.6% and 4.1–8.8% compared with S0 and B0, respectively, but they did not significantly affect the bacterial diversity. The redundancy analysis (RDA) suggested that EC, SWC, pH, AN and AP were the most critical factors driving the change of soil bacterial community structure accounting for 69.9%, 64.7% 61.6%, 57.0% and 51.8% of the variation, respectively. Therefore, our results provided valuable insight into how biochar addition would affect alfalfa yield and soil properties, including bacterial community richness and composition and structure of saline soils in arid regions.
Tunable Topological Lasing of Circularly Polarized Light in a Soft‐Matter‐Based Superlattice
Authors: Wang Yu , Yang Donghao , Gao Shaohua , Zhang Xinzheng , Drevensek‐Olenik Irena , Wu Qiang , Chemingui Marouen , Chen Zhigang , Xu Jingjun 
Published: March 2023

AbstractThe advance of topological photonics has heralded a revolution for manipulating light as well as for the development of novel photonic devices such as topological insulator lasers. Here, the robust topological interface state lasing in a polymer‐cholesteric liquid crystal superlattice at the visible regime is demonstrated. By use of the femtosecond‐laser direct‐writing and self‐assembling techniques, the micron‐sized superlattice is established with a controlled mini‐band structure and a topological interface defect, thereby achieving a low threshold for robust topological lasing at about 0.4 µJ (722 W·mm−2). Thanks to the chiral liquid crystal, not only is the circularly polarized lasing readily achieved, but the emission wavelength is thermally tuned. The results bring about the possibility to realize tunable, circularly polarized, compact, and integrated topological photonic devices at low cost, as well as to engineer an ideal platform for exploring topological physics that involves light–matter interaction in soft‐matter environments.
Circular RNA ACACA negatively regulated p53‐modulated mevalonate pathway to promote colorectal tumorigenesis via regulating miR‐193a/b‐3p/HDAC3 axis
Authors: He Fengjiao , Liu Qiong , Liu Huan , Pei Qian , Zhu Hong 
Published: March 2023

AbstractThis study aimed to explore the biological functions and underlying mechanism of circRNA acetyl‐CoA carboxylase alpha (circACACA) in colorectal cancer (CRC). The RNA and protein levels were detected by qRT‐PCR and western blot assays. The malignant capacities of CRC cells were analyzed by cell counting kit‐8 (CCK‐8), colony formation, flow cytometry, and transwell assays. The target relationship between miR‐193a/b‐3p and circACACA/histone deacetylase 3 (HDAC3) was determined by luciferase reporter assay and RNA immunoprecipitation. The binding of HDAC3 to the p53 promoter was validated by chromatin immunoprecipitation (ChIP). CRC cell growth and lung metastasis were evaluated in nude mice in vivo. High expression of circACACA was found in CRC tissues and cells, which was closely associated with the advanced tumor, lymph node, metastasis (TNM) stage, metastasis, and low overall survival rate. circACACA downregulation effectively delayed CRC cell proliferation and metastasis, but triggered apoptosis via inactivating the mevalonic acid (MVA) pathway. However, circACACA overexpression resulted in the opposite effects. Mechanistically, circACACA enhanced HDAC3 expression through sponging miR‐193a/b‐3p, which activated the MVA pathway via inhibiting the acetylation and transcription of p53. Moreover, rescue experiments confirmed that miR‐193a/b‐3p inhibition reversed the inhibitory effect of circACACA deficiency on CRC growth and metastasis. Moreover, circACACA overexpression‐mediated malignant phenotypes of CRC cells were abrogated by HDAC3 knockdown. circACACA promoted CRC progression via regulating the miR‐193a/b‐3p/HDAC3/p53 axis to activate the MVA pathway, providing evidence for circACACA as a promising therapeutic target for CRC.
A drug utilization study of thiocolchicoside‐containing medicinal products for systemic use in France and Italy: A cross‐sectional electronic medical records database study
Authors: Druet‐Cabanac Annie , Sophie Jouaville Laurence , Afshari Reza , Sahnoun Rachid , Kouao‐Kanga Germain , Toussi Massoud , Granados Denis 
Published: March 2023

AbstractPurposeThe risk minimization measures (RMM) for systemic use of thiocolchicoside (TCC) was implemented across Europe during 2014–2016. RMM included restriction of use in age <16 years, maximum dose and duration, chronic conditions, contraindication in pregnancy, lactation or in women of childbearing potential [WOCBP] without appropriate contraception. The current Drug Utilization Study was aimed to describe the prescribing practices of TCC in France and Italy.MethodThe study analyzed data (demographic, prescription, diagnosis, and concomitant treatment) from electronic medical record databases. It compares drug utilization during pre‐implementation (baseline: year 2013) and post‐implementation (years 1, 2, and 3) of RMM. This study included panels of general practitioners (FGP) and rheumatologists (FRH) in France and Italy (IGP).ResultsTCC was largely prescribed as adjuvant therapy in both pre‐implementation (FGP: 93.5%, FRH: 88.8%, IGP: 86.6%) and post‐implementation (FGP: 92.3%, FRH: 89.5%, IGP: 89.0%) periods. Prescribing patterns were different in France and Italy, with FGP and FRH mainly prescribing oral formulation (>95% and >80%, respectively), while IGP prescribing intramuscular formulation (>70%). Prescriptions to patients aged ≥16 years were >99% in all panels during both periods. An improvement was observed in compliance with treatment duration for oral formulation in the FGP panel post‐implementation versus pre‐implementation (66.2% vs. 46.7%; p < 0.001). There was no change in prescription rate post RMM implementation in pregnant (FGP: 0.5%, IGP: 4.7%) and in WOCBP without appropriate contraception (FGP: 89.3%, IGP: 93.4%).ConclusionThese results highlighted changes in prescribing practices of TCC after RMM implementation, which varied across panels and measures.
Bias amplification of unobserved confounding in pharmacoepidemiological studies using indication‐based sampling
Authors: Ahlqvist Viktor H. , Madley‐Dowd Paul , Ly Amanda , Rast Jessica , Lundberg Michael , Jónsson‐Bachmann Egill , Berglind Daniel , Rai Dheeraj , Magnusson Cecilia , Lee Brian K. 
Published: March 2023

AbstractPurposeEstimating causal effects in observational pharmacoepidemiology is a challenging task, as it is often plagued by confounding by indication. Restricting the sample to those with an indication for drug use is a commonly performed procedure; indication‐based sampling ensures that the exposed and unexposed are exchangeable on the indication—limiting the potential for confounding by indication. However, indication‐based sampling has received little scrutiny, despite the hazards of exposure‐related covariate control.MethodsUsing simulations of varying levels of confounding and applied examples we describe bias amplification under indication‐based sampling.ResultsWe demonstrate that indication‐based sampling in the presence of unobserved confounding can give rise to bias amplification, a self‐inflicted phenomenon where one inflates pre‐existing bias through inappropriate covariate control. Additionally, we show that indication‐based sampling generally leads to a greater net bias than alternative approaches, such as regression adjustment. Finally, we expand on how bias amplification should be reasoned about when distinct clinically relevant effects on the outcome among those with an indication exist (effect‐heterogeneity).ConclusionWe conclude that studies using indication‐based sampling should have robust justification ‐ and that it should by no means be considered unbiased to adopt such approaches. As such, we suggest that future observational studies stay wary of bias amplification when considering drug indications.
Finite element theoretical and experimental study on the dynamic characteristics of material extrusion thin plates
Authors: Jiang Shijie , Chen Chuangchuang , Li Changyou , Zhao Chunyu 
Published: March 2023

AbstractMaterial extrusion (ME), one of the most rapidly developing additive manufacturing techniques, has been widely used in the field of aerospace, medical, industrial design, and so on. Since the working environment is increasingly complex, it is urgent to study the ME products' dynamic performances to determine their reliability. In this article, a finite element theoretical model of ME thin plate was established with the porosity defect taken into account. A modal test system was set up and the multiple‐input single‐output method was used to obtain the measured data. The scanning electron microscopy (SEM) analysis on the samples confirmed that the dynamic characteristic was improved as the extrusion temperature increased. In addition, the sensitivity analysis was carried out on the model to predict how the samples' elastic modulus, Poisson's ratio, and density affect their dynamic characteristics. The results show that the proposed model is reliable to give accurate predictions on the dynamic characteristics of ME thin plates. With the increase of extrusion temperature, the samples' natural frequency will increase, and the vibration response will decrease. The sensitivity analysis indicates that increasing elastic modulus or Poisson's ratio can increase the natural frequency of ME thin plate but decrease the vibration response. While increasing the density will decrease both the natural frequency and vibration response.
The association between school staff's coping strategies following a student's suicide, school climate, and previous experience with suicide
Authors: Fire Noa Tiech , Alkalay Sarit , Gvion Yari , Zalsman Gil 
Published: March 2023

AbstractThis article examines whether and to what extent school climate and previous experience of attempted suicide or suicide of someone close, affect the strategies adopted by Israeli school staff members in coping with a student's suicide. Participants included 84 homeroom teachers, principals, counselors, and psychologists who work at schools where a student had died by suicide during the five preceding years. Our findings show that optimal school climate predicts elevated levels of overall coping strategies and higher problem‐focused strategies. Additionally, staff members who previously experienced suicide or attempted suicide of a close person exhibit lower levels of coping strategies, in general, and of emotion‐focused strategies in particular. Thus, they can be considered a risk group for less adaptive adjustment following a student's suicide. Finally, previous incidents of suicide or attempted suicide of a close person do not moderate the link between optimal school climate and coping strategies. Thus, optimal school climate has a robust and positive effect on school staff's coping abilities and hence may be considered a protective factor for the risk group we identified, namely school staff who had previous experience of someone close who attempted suicide or died by suicide.