bluuebunny/crossref_metadata_2025_split huggingface dataset:
Cellular and molecular heterogeneities and signatures, and pathological trajectories of fatal COVID-19 lungs defined by spatial single-cell transcriptome analysis
Authors: Das Arun , Meng Wen , Liu Zhentao , Hasib Md Musaddaqul , Galloway Hugh , Ramos da Silva Suzane , Chen Luping , Sica Gabriel L , Paniz-Mondolfi Alberto , Bryce Clare , Grimes Zachary , Sordillo Emilia Mia , Cordon-Cardo Carlos , Rivera Karla Paniagua , Flores Mario , Chiu Yu-Chiao , Huang Yufei , Gao Shou-Jiang 
Published: February 2023

AbstractDespite intensive studies during the last 3 years, the pathology and underlying molecular mechanism of coronavirus disease 2019 (COVID-19) remain poorly defined. Here, we examined postmortem COVID-19 lung tissues by spatial single-cell transcriptome analysis (SSCTA). We identified 18 major parenchymal and immune cell types, all of which are infected by SARS-CoV-2. Compared to the non-COVID-19 control, COVID-19 lungs have reduced alveolar cells (ACs), and increased innate and adaptive immune cells. Additionally, 19 differentially expressed genes in both infected and uninfected cells across the tissues mirror the altered cellular compositions. Spatial analysis of local infection rates revealed regions with high infection rates that are correlated with high cell densities (HIHD). The HIHD regions express high levels of SARS-CoV-2 entry-related factors including ACE2, FURIN, TMPRSS2, and NRP1, and co-localized with organizing pneumonia (OP) and lymphocytic and immune infiltration that have increased ACs and fibroblasts but decreased vascular endothelial cells and epithelial cells, echoing the tissue damage and wound healing processes. Sparse non- negative matrix factorization (SNMF) analysis of neighborhood cell type composition (NCTC) features identified 7 signatures that capture structure and immune niches in COVID-19 tissues. Trajectory inference based on immune niche signatures defined two pathological routes. Trajectory A progresses with primarily increased NK cells and granulocytes, likely reflecting the complication of microbial infections. Trajectory B is marked by increased HIHD and OP, possibly accounting for the increased immune infiltration. The OP regions are marked by high numbers of fibroblasts expressing extremely high levels of COL1A1 and COL1A2. Examination of single-cell RNA-seq data (scRNA-seq) from COVID-19 lung tissues and idiopathic pulmonary fibrosis (IPF) identified similar cell populations primarily consisting of myofibroblasts.Immunofluorescence staining revealed the activation of IL6-STAT3 and TGF-²-SMAD2/3 pathways in these cells, which likely mediate the upregulation of COL1A1 and COL1A2, and excessive fibrosis in the lung tissues. Together, this study provides an SSCTA atlas of cellular and molecular signatures of fatal COVID-19 lungs, revealing the complex spatial cellular heterogeneity, organization, and interactions that characterized the COVID-19 lung pathology.
Comparison of Machine Learning Methods in Mild Cognitive Impairment Prediction for Cancer Patients Using EHR Data
Authors: Zhang Xiaodan , Witteveen-Lane Martin , Li Yanzeng , Kulkarni Omkar , Chesla Dave , Chen Bin 
Published: February 2023

AbstractCancer and dementia are common in aging populations. Mild cognitive impairment (MCI) is a stage between the cognitive changes of normal aging and dementia that can lead to a decline in quality of life. With the substantial improvement of survival in many cancers, maintaining a high quality of life has become a new goal in cancer care. Identifying those patients with a high risk of developing MCI may facilitate early intervention and further improve patient care. The objective of this study is to survey machine learning techniques and AutoML to model the early detection of MCI in patients with cancers using the features which are known risk factors in dementia and accessible in the electronic health records (EHR). We compared multiple machine learning methods and explored AutoML to predict 1-year risk of MCI for cancer patients. Among 27 models, XGBoost in AutoML gave the highest AUC (0.79), suggesting the superiority of using automated machine learning tools to search for the best model and parameters. The feature importance analysis revealed that cancer patients with brain malignancy, hypertension, or cardiovascular diseases are more likely to develop MCI. The overall poor performance indicates more efforts should be made to improve data quality and increase features and sample size.
Conservation implications of elucidating the Korean wolf taxonomic ambiguity through whole-genome sequencing
Authors: Hernández-Alonso Germán , Ramos-Madrigal Jazmín , Sun Xin , Scharff-Olsen Camilla Hjorth , Sinding Mikkel-Holger S. , Martins Nuno F. , Ciucani Marta Maria , Mak Sarah S. T. , Lanigan Liam Thomas , Clausen Cecilie G. , Bhak Jong , Jeon Sungwon , Kim Changjae , Eo Kyung Yeon , Cho Seong-Ho , Bazartseren Boldgiv , Gantulga Gankhuyag , Unudbayasgalan Zunduibaatar , Kosintsev Pavel A. , Stenøien Hans K. , Gilbert M. Thomas P. , Gopalakrishnan Shyam 
Published: February 2023

AbstractThe taxonomic status of the now likely extirpated Korean Peninsula wolf has been extensively debated, with some arguing it represents an independent wolf lineage,Canis coreanus. To investigate the Korean wolf genetic affiliations and their taxonomic implications, we sequenced and analysed the genomes of a historical Korean wolf dated to the beginning of the 20th century, and a captive wolf originally located at the Pyongyang Central Zoo. Our results indicated that the Korean wolf bears similar genetic ancestry to other regional East Asian populations, therefore suggesting it is not a distinct taxonomic lineage. We identified regional patterns of wolf population structure and admixture in East Asia with potential conservation consequences in the Korean Peninsula and on a regional scale. We find the Korean wolf has similar diversity and inbreeding to other East Asian wolves. Finally, we show that, in contrast to the historical sample, the captive wolf is more genetically similar to wolves from the Tibetan Plateau, hence, Korean wolf conservation programs might not benefit from the inclusion of this specimen.
Interplay between perceived value, trust and continuance intention: evidence in the sharing economy
Authors: Tumaku John , Ren Jianxin , Boakye Kwabena Gyasi , Ofori Kwame Simpe , Abubakari Aidatu 
Published: February 2023

Purpose
Over the past decade, research into sharing economy platforms has gained prominence. The purpose of this study is to investigate the role of perceived value (both hedonic and utilitarian) in attracting consumer engagement in the sharing economy, as well as its link with trust.

Design/methodology/approach
This study used a survey method to empirically tested the proposed model using the partial least squares approach to structural equation modeling on data from 320 DiDi app users.

Findings
The study's findings revealed that both hedonic and utilitarian value had a significant effect on satisfaction and trust in the platform. Although the results showed no effect of hedonic and utilitarian values on trust in driver, the authors found trust in driver and platform, and satisfaction had significant influences on users’ continued intention to use the Taxi-hailing app. Interestingly, this study suggests that trust in the platform is transferred to trust in the driver.

Originality/value
The results from the Necessary Condition Analysis shows that satisfaction and utilitarian value are necessary conditions of continuance intention.
Antecedents and consequences of strategic online-reputation management: moderating effect of online tools
Authors: Peco-Torres Francisco , Polo-Peña Ana I. , Frías Jamilena Dolores M. 
Published: February 2023

Purpose
This study aims to examine the antecedents and consequences of strategic online-reputation management among tourism firms. It is proposed that customer relationship management (CRM) is an antecedent of strategic online-reputation management, brand equity is a consequence of strategic online-reputation management and the use of online tools moderates the effect of strategic online-reputation management and CRM on brand equity.

Design/methodology/approach
A quantitative empirical study is conducted among online marketing managers of chain hotels. Structural equation modeling (SEM) is used to verify the proposed relationships.

Findings
The results show that CRM is an antecedent of strategic online-reputation management, and that strategic online-reputation management and CRM exert a positive effect on brand equity among firms that frequently use a range of online tools. No such effect is found in cases of limited usage of online tools.

Practical implications
Tourism firms seeking to achieve greater brand equity by means of strategic online-reputation management should implement CRM and make extensive use of online tools.

Originality/value
The main contributions of this study are that it analyzes the antecedents and consequences of strategic online-reputation management jointly and empirically; studies the moderating role of the use of online tools in the effect of online strategies – such as online-reputation management and CRM – on brand equity; and studies the consequences of CRM in online media.
Muslim Indonesian women entrepreneurs: a factor analysis of business performance
Authors: ChoudhuryKaul Sanjukta , Supriyadi Ono , Fahlevi Nabilla 
Published: February 2023

Purpose
Muslim Indonesian women entrepreneurs (MIWEs) lie at the intersection of religion and gender. Given the growing participation of women in entrepreneurship and economic stability in Indonesia, the purpose of this study is to identify the factors (and their significance) influencing MIWEs’ business performance.

Design/methodology/approach
A survey of 101 MIWEs was conducted, and results were analyzed via structural modelling equation using SmartPLS 3.

Findings
The findings of this study suggest that, within the macro frame of the moderate version of Islam practiced and women’s economic engagement, women business owners’ skills and religious factors significantly influence their business performance. Factor analysis indicates that the role of the veil, Shariah guidance for business and managing stress through the practice of Salah (prayer) are important for MIWEs. However, this study also indicates that, in addition to religious factors, MIWEs’ own effective and participative leadership style, honesty and fairness reputation in business dealings and a good market image are also significant variables affecting business performance. This is in contrast to past studies, where the role of environmental factors such as access to capital, family and government has been shown to have a stronger influence on Muslim women entrepreneurs’ business performance.

Originality/value
Because of Indonesia’s unique pluralistic national context, along with increasing women’s economic participation, MIWEs emerge as a distinct category of entrepreneurs who integrate religion and their own skills to navigate their business performance.
A preliminary post-occupancy evaluation of the built-environment in office buildings: a case study from Saudi Arabia
Authors: Hassanain Mohammad A. , Mahroos Mohammed S. 
Published: February 2023

PurposePost-occupancy evaluation (POE) of office buildings, accommodating large number of users, is significantly demanded, to ascertain the provision and upkeep of users' requirements. This paper presents the findings of a POE of an office building located in Saudi Arabia, as a case study.Design/methodology/approachA mixed approach of qualitative and quantitative research activities were conducted to achieve the objective of this study. Previous studies were analyzed to identify 28 performance indicators, grouped under seven technical and functional performance elements in office buildings. A walkthrough served to gain familiarity with the building, and identify the obvious performance defects. Interviews were conducted to solicit the users' perceptions about the performance elements, and initiate discussions based on the findings of the walkthrough. A user satisfaction survey was then deployed to quantify the user's feedback on the performance of the building.FindingsUsers of the case study office building were generally satisfied with all the technical performance elements, except visual comfort. They were also dissatisfied with two functional performance elements, namely office layout and building equipment.Practical implicationsRecommendations were developed to improve the performance of the case study building. The approach followed and the defined indicators can be of practical value to design professionals, administrators and facilities managers, responsible for the design and operation of office buildings.Originality/valueOffice buildings are valuable business capital for their corporate organizations. This study contributes to the facilities management literature, through providing a systematic approach for the quality and performance appraisal of the performance elements of office buildings.
The role of leadership in effective implementation of quality assurance mechanisms in higher education: an exploratory case study from Pakistan
Authors: Ahmad Sohail , Ahmed Afaq 
Published: February 2023

Purpose
This study aims to explore whether and how leaders’ attributes, understanding of and attitude towards quality assurance (QA) shape their practices towards effective implementation of QA in universities.

Design/methodology/approach
Using qualitative case study, the data were collected from nine institutional leaders purposively selected from two universities. The data, collected through semi-structured interviews, were analysed using thematic analysis technique.

Findings
The findings revealed that personal attributes of leaders such as understanding of QA, attitude towards QA and practices shape leadership style either as transformational or compliance leader. The transformational leader implements QA procedures with the intention of bringing improvement in teaching learning and research quality. In contrast, the compliance leaders are primarily concerned with implementation of QA procedures merely to fulfil the requirements of an external regulatory body.

Research limitations/implications
This study acknowledges that certain other factors (e.g. demographics, institutional policies or practices) might have an influence on leadership practices. Using the framework of this study, further quantitative and/or mixed methods research can expand by bringing more factors that can shape leadership practices.

Originality/value
The role of leadership is significant to the success of any university. A plethora of studies have reported various aspects of successful leadership in higher education (HE). However, in the changing nature of HE due to the emergence of formal QA, there is paucity of research that focusses on the role of leader’s attributes in the effective implementation of QA procedures.
The symbolic meanings and practice of shopping malls in Hong Kong beyond consumption: a case study of Kwai Chung Plaza
Authors: Cheung Siu Fung 
Published: February 2023

PurposeThis study aims to examine the symbolic meanings of the local shopping malls inscribed by the shoppers from the sociocultural lens other than merely departing from the economic dimensions.Design/methodology/approachIn this research, Kwai Chung Plaza was adopted as the case study. The author interviewed 12 local shoppers through an in-depth individual interview. All interviewees were selected through convenience and snowball sampling. All names of the participants in this paper are kept pseudonymous to protect their privacy.FindingsAn integrated model, which consists of three major levels, namely, the spatial, individual and community levels, has been formed to show that the shopping mall was not merely a place for buy-and-sell transactions but rather a place that could be understood as an extension of the street, a body of collective memories and a place for Hongkongers.Research limitations/implicationsThe most significant limitation of this study was the lack of a diversified demographic profile adding that the sample was restricted to the young generation. It is recommended that future research should consider including interviews with middle-aged and old-aged shoppers to ensure the generalizability of results. Future research may also consider examining other small/medium-sized shopping malls for comparison.Originality/valueThe findings demonstrated the diversified social roles and functions of the local shopping malls in a community. In addition, the present study, to the author’s knowledge, is one of the few scholarly discussions on small/medium-sized shopping malls from a sociocultural perspective.
Efficacy and safety of osimertinib plus anlotinib in advanced non‐small‐cell lung cancer patients after drug resistance
Authors: Wang Mingzhao , Zhao Jun , Chen Tong , Hu Xingsheng , Wang Lin , Shi Yuankai , Liu Yutao 
Published: February 2023

AbstractObjectiveTo retrospectively analyze the efficacy and safety of osimertinib combined with anlotinib in the treatment of advanced non‐small‐cell lung cancer (NSCLC) after drug resistance, and to explore the related factors affecting the efficacy.MethodsThe clinical data of 34 patients with advanced NSCLC who received osimertinib combined with anlotinib as three or more lines of treatment in the Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College from June 2019 to March 2022 were collected, and the therapeutic efficacy and safety were analyzed.ResultsA total of 34 advanced NSCLC patients met the inclusion criteria. The objective response rate was 20.6%, the disease response rate was 88.2%, the median overall survival was 19.0 months, and the median progression‐free survival was 6.0 months. The common adverse events were mainly grade 1–2, and only three cases (11.1%) of adverse events were grade 3, including hypertension, proteinuria, and vomiting. No grade 4 or above adverse events were observed. Multivariate Cox regression analysis showed that the Eastern Cooperative Oncology Group Performance Status score and bone metastasis were independent prognostic factors for osimertinib combined with anlotinib as three or more lines of treatment in advanced NSCLC.ConclusionsOsimertinib combined with anlotinib as three or more lines of treatment in advanced NSCLC was effective and adverse events were tolerable.
Adult asthma with symptomatic eosinophilic inflammation is accompanied by alteration in gut microbiome
Authors: Gu Bon‐Hee , Choi Jun‐Pyo , Park Tansol , Kim A‐Sol , Jung Ho Young , Choi Doo Young , Lee Sang Jin , Chang Yoon‐Seok , Kim Myunghoo , Park Han‐Ki 
Published: February 2023

AbstractBackgroundAccumulating evidence suggests that the gut microbiome is associated with asthma. However, altered gut microbiome in adult asthma is not yet well established. We aimed to investigate the gut microbiome profiles of adult asthmatic patients with symptomatic eosinophilic inflammation.MethodsThe 16 s rRNA gene metagenomic analysis of feces in the symptomatic eosinophilic asthma group (EA, n = 28) was compared with the healthy control (HC, n = 18) and the chronic cough control (CC, n = 13). A correlation analysis between individual taxa and clinical markers was performed within the EA group. Changes in the gut microbiome were examined in patients with significant symptom improvement in the EA group.ResultsThe relative abundances of Lachnospiraceae and Oscillospiraceae significantly decreased and Bacteroidetes increased in the EA group. Within EA group, Lachnospiraceae was negatively correlated with indicators of type 2 inflammation and lung function decline. Enterobacteriaceae and Prevotella was positively associated with type 2 inflammation and lung function decline, respectively. The abundance of predicted genes associated with amino acid metabolism and secondary bile acid biosynthesis was diminished in the EA group. These functional gene family alterations could be related to gut permeability, and the serum lipopolysaccharide concentration was actually high in the EA group. EA patients with symptom improvement after 1 month did not show a significant change in the gut microbiome.ConclusionsSymptomatic eosinophilic adult asthma patients showed altered the gut microbiome composition. Specifically, a decrease in commensal clostridia was observed, and a decrease in Lachnospiraceae was correlated with blood eosinophilia and lung function decline.
Inpatient and outpatient treatment by specialist infant nutrition centres in Argentina effectively reduced child malnourishment
Authors: Santiano Flavia Eliana , Fernandez‐Muñoz Juan Manuel , Pistone‐Creydt Virginia , Zoppino Felipe Carlos Martín , López‐Fontana Constanza Matilde , Carón Rubén Walter 
Published: February 2023

AbstractAimWe evaluated the impact of inpatient and outpatient treatment provided by an infant nutrition foundation in Las Heras, Mendoza, Argentina and identified the factors that influenced nutritional recovery.MethodsThis 2010–2018 retrospective study was based on 300 children up to 5 years of age with primary malnutrition, who were treated by an inpatient recovery centre, then an outpatient prevention centre. We analysed the children's height, weight, psychomotor development and living conditions when they were admitted, discharged and had received 1 year of outpatient treatment. There were full data on 241 children and just admission and discharge data for 59.ResultsThe children's mean age on admission and weight were 14.8 ± 12.4 months and 6.9 ± 2.3 kg and they stayed in hospital for a mean of 59.5 ± 49.7 days. We observed a significant increase in the weight‐for‐age, height‐for‐age and weight‐for‐height z‐scores when all three time points were compared (p < 0.001). Psychomotor development improved considerably in all patients after treatment. The factors that negatively influenced nutritional recovery were higher age at admission, suboptimal breastfeeding practices, low birth weight, longer hospital stays, younger maternal age and overcrowded housing.ConclusionCombining inpatient recovery and outpatient preventive treatment was effective for undernourished children in Argentina.
Genetic stratification reveals COL4A variants and spontaneous remission in Egyptian children with proteinuria in the first 2 years of life
Authors: Elshafey Samar Atef , Thabet Mohamed Alaa Eldin Hassan , Abo Elwafa Reham Abdel Haleem , Schneider Ronen , Shril Shirlee , Buerger Florian , Hildebrandt Friedhelm , Fathy Hanan M 
Published: February 2023

AbstractAimThe earlier the onset of proteinuria, the higher the incidence of genetic forms. Therefore, we aimed to analyse the spectrum of monogenic proteinuria in Egyptian children presenting at age <2 years.MethodsThe results of 27‐gene panel or whole‐exome sequencing were correlated with phenotype and treatment outcomes in 54 patients from 45 families.ResultsDisease‐causing variants were identified in 29/45 (64.4%) families. Mutations often occurred in three podocytopathy genes: NPHS1, NPHS2 and PLCE1 (19 families). Some showed extrarenal manifestations. Additionally, mutations were detected in 10 other genes, including novel variants of OSGEP, SGPL1 and SYNPO2. COL4A variants phenocopied isolated steroid‐resistant nephrotic syndrome (2/29 families, 6.9%). NPHS2 M1L was the single most common genetic finding beyond the age of 3 months (4/18 families, 22.2%). Biopsy results did not correlate with genotypes (n = 30). On renin–angiotensin–aldosterone system antagonists alone, partial and complete remission occurred in 3/24 (12.5%) patients with monogenic proteinuria each, whereas 6.3% (1/16) achieved complete remission on immunosuppression.ConclusionGenotyping is mandatory to avoid biopsies and immunosuppression when proteinuria presents at age <2 years. Even with such a presentation, COL4A genes should be included. NPHS2 M1L was prevalent in Egyptian children (4 months–2 years) with proteinuria, demonstrating precision diagnostic utility.