bluuebunny/crossref_metadata_2025_split huggingface dataset:
Post-glacial recolonization and multiple scales of secondary contact contribute to contemporary Atlantic salmon (Salmo salar) genomic variation in North America
Authors: Nugent Cameron M. , Kess Tony , Langille Barbara L. , Beck Samantha V. , Duffy Steven , Messmer Amber , Smith Nicole , Lehnert Sarah J. , Wringe Brendan F. , Kent Matthew , Bentzen Paul , Bradbury Ian R. 
Published: August 2023

ABSTRACTAimIn northern environments, periods of isolation during Pleistocene glaciations and subsequent recolonization and secondary contact have had a significant influence on contemporary diversity of many species. The recent advent of high-resolution genomic analyses allows unprecedented power to resolve genomic signatures of such events in northern species. Here, we provide the highest resolution genomic characterization of Atlantic salmon in North America to infer glacial refugia and the geographic scales of postglacial secondary contact.LocationNorth America.TaxonAtlantic salmon,Salmo salar.MethodsSamples were collected for 5455 individuals from 148 populations encompassing the majority of Atlantic salmon’s native range in North America, from Labrador to Maine. Individuals were genotyped using a 220K SNP array aligned to the Atlantic salmon (Salmo salar) genome. Spatial genetic structure (PCA, k-means clustering, admixture) was evaluated in conjunction with genomic comparisons of identified lineages to infer the refugia during the last glacial maximum and regions of secondary contact following recolonization.ResultsSpatial genomic analyses identified three phylogeographic groups, consistent with the northward recolonization from two southern glacial refugia in North America (a western Maritime lineage and an eastern Newfoundland and Labrador lineage), with subsequent differentiation of the eastern lineage into two separate groups. Secondary contact among these North American groups was present within the northern Gulf of St. Lawrence and evidence of trans-Atlantic secondary contact was detected within the eastern Newfoundland and Labrador lineage. Comparison of groups from insular Newfoundland with those from mainland Labrador suggests genomic regions displaying high differentiation were characterized by elevated European admixture, suggesting a possible role of European secondary contact in population divergence.Main ConclusionsThese findings present the first evidence suggesting that genomic diversity in extant North American Atlantic salmon populations has resulted from allopatric isolation in two glacial refugia followed by both regional and trans-Atlantic recolonization and secondary contact and demonstrate the power of genomic tools to resolve historical drivers of diversity in wild populations.
Neural and behavioral organization of rapid eye movement sleep in zebrafish
Authors: Choudhary Vikash , Heller Charles R. , Aimon Sophie , de Sardenberg Schmid Lílian , Robson Drew N. , Li Jennifer M. 
Published: August 2023

ABSTRACTSleep is ubiquitous across the animal kingdom. However, the definition for sleep varies significantly across species. In organisms such asC. elegans, Drosophila, and zebrafish, sleep is classically defined as periods of locomotor quiescence with an increased arousal threshold1–6. In mammals, sleep is further subdivided into rapid eye movement (REM) and non-rapid eye movement (NREM) sleep, but the evolutionary origin of these two sleep states remains unclear7–12. Using longitudinal, high-resolution imaging of naturalistic sleep-wake behavior, we find that the classical definition of larval zebrafish sleep encompasses two clearly distinct quiescent states, one with rapid eye movements (qREM) and one without rapid eye movements (qNREM). Remarkably, qREM states are maintained in congenitally blind larval zebrafish, suggesting that qREM emerged during vertebrate evolution not as a response to internally generated visual imagery (i.e., the “scanning hypothesis”)13, but potentially as a strategy to maintain oculomotor stimulation during rest14. Lastly, using brain-wide imaging in freely swimming animals, we find that, in contrast to fixed point attractor models of sleep developed in invertebrates15, population-level activity during zebrafish qREM unfolds along smooth committed trajectories through state-space. This work suggests a significant rethinking of the behavioral and neural organization of sleep in early vertebrates and the emergence of REM during vertebrate evolution.
WITHDRAWN: Sindbis Virus in Wild Pigeons, Thailand
Authors: Yodjan Naluepanat , Wongwatthanacharat Suphawat , Khamsri Boonruang 
Published: August 2023

Withdrawal StatementThe authors have withdrawn their manuscript owing to some missteps or inaccuracies discovered in the methodology that may significantly impact the findings. Therefore, the authors do not wish this work to be cited as a reference for the project. If you have any questions, please contact the corresponding author.
The β6 Integrin Negatively Regulates TLR7-Mediated Epithelial Immunity via Autophagy During Influenza A Virus Infection
Authors: Smith Maria , Meliopoulos Victoria , Tan Shaoyuan , Bub Theresa , Brigleb Pamela H. , Sharp Bridgett , Crawford Jeremy Chase , Prater Mollie S. , Pruett-Miller Shondra M. , Schultz-Cherry Stacey 
Published: August 2023

SUMMARYIntegrins are essential surface receptors that sense extracellular changes to initiate various intracellular signaling cascades. The rapid activation of the epithelial-intrinsic β6 integrin during influenza A virus (IAV) infection has been linked to innate immune impairments. Yet, how β6 regulates epithelial immunity remains undefined. Here, we identify the role of β6 in mediating the Toll-like receptor 7 (TLR7) through the regulation of intracellular trafficking. We demonstrate that deletion of the β6 integrin in lung epithelial cells significantly enhances the TLR7-mediated activation of the type I interferon (IFN) response during homeostasis and respiratory infection. IAV-induced β6 facilitates TLR7 trafficking to lysosome-associated membrane protein (LAMP2a) components, leading to a reduction in endosomal compartments and associated TLR7 signaling. Our findings reveal an unappreciated role of β6-induced autophagy in influencing epithelial immune responses during influenza virus infection.
Pervasive nuclear envelope ruptures precede ECM signaling and disease onset without activating cGAS-STING in Lamin-cardiomyopathy mice
Authors: En Atsuki , Bogireddi Hanumakumar , Thomas Briana , Stutzman Alexis , Ikegami Sachie , LaForest Brigitte , Almakki Omar , Pytel Peter , Moskowitz Ivan P. , Ikegami Kohta 
Published: August 2023

SUMMARYNuclear envelope (NE) ruptures are emerging observations in Lamin-related dilated cardiomyopathy, an adult-onset disease caused by loss-of-function mutations in Lamin A/C, a nuclear lamina component. Here, we tested a prevailing hypothesis that NE ruptures trigger pathological cGAS-STING cytosolic DNA-sensing pathway, using a mouse model of Lamin-cardiomyopathy. Reduction of Lamin A/C in cardiomyocytes of adult mice caused pervasive NE ruptures in cardiomyocytes, preceding inflammatory transcription, fibrosis, and fatal dilated cardiomyopathy. NE ruptures were followed by DNA damage accumulation without causing immediate cardiomyocyte death. However, cGAS-STING-dependent inflammatory signaling remained inactive. DeletingcGasorStingdid not rescue cardiomyopathy. The lack of cGAS-STING activation was likely due to the near absence of cGAS expression in adult cardiomyocytes at baseline. Instead, extracellular matrix (ECM) signaling was activated and predicted to initiate pro-inflammatory communication from Lamin-reduced cardiomyocytes to fibroblasts. Our work nominates ECM signaling, not cGAS-STING, as a potential inflammatory contributor in Lamin-cardiomyopathy.
Tau fibrils induce nanoscale membrane damage and nucleate cytosolic tau at lysosomes
Authors: Rose Kevin , Jepson Tyler , Shukla Sankalp , Maya-Romero Alex , Kampmann Martin , Xu Ke , Hurley James H. 
Published: August 2023

AbstractThe prion-like spread of protein aggregates is a leading hypothesis for the propagation of neurofibrillary lesions in the brain, including the spread of tau inclusions associated with Alzheimer’s disease. The mechanisms of cellular uptake of tau seeds and subsequent nucleated polymerization of cytosolic tau are major questions in the field, and the potential for coupling between the entry and nucleation mechanisms has been little explored. We found that in primary astrocytes, endocytosis of tau seeds leads to their accumulation in lysosomes. This in turn leads to lysosomal swelling, deacidification and recruitment of ESCRT proteins, but not Galectin-3, to the lysosomal membrane. These observations are consistent with nanoscale damage of the lysosomal membrane. Using live cell and STORM, imaging, nucleation of cytosolic tau occurs primarily at the lysosome membrane under these conditions. These data suggest that tau seeds escape from lysosomes via nanoscale damage rather than wholesale rupture, and that nucleation of cytosolic tau commences as soon as tau fibril ends emerge from the lysosomal membrane.
L-DOS47 enhances response to immunotherapy in pancreatic cancer tumor
Authors: Jardim-Perassi Bruna Victorasso , Irrera Pietro , Abrahams Dominique , Estrella Veronica C. , Ordway Bryce , Byrne Samantha R. , Ojeda Andrew A. , Whelan Christopher J. , Kim Jongphil , Beatty Matthew S. , Damgaci-Erturk Sultan , Longo Dario Livio , Gaspar Kim J. , Siegers Gabrielle M. , Centeno Barbara A. , Lau Justin Y.C. , Ibrahim-Hashim Arig , Pilon-Thomas Shari A. , Gillies Robert J. 
Published: August 2023

AbstractAcidosis is an important immunosuppressive mechanism that leads to tumor growth. Therefore, we investigated the neutralization of tumor acidity to improve immunotherapy response. L-DOS47, a new targeted urease immunoconjugate designed to neutralize tumor acidity, has been well tolerated in phase I/IIa trials. L-DOS47 binds CEACAM6, a cell surface protein highly expressed in gastrointestinal cancers, allowing urease to cleave endogenous urea into two NH4+ and one CO2, thereby raising local pH. To test the synergetic effect of neutralizing tumor acidity with immunotherapy, we developed a pancreatic orthotopic murine tumor model (KPC961) expressing human CEACAM6. Our results demonstrate that combining L DOS47 with anti-PD1 significantly increases the efficacy of anti-PD1 monotherapy, reducing tumor growth for up to 4 weeks.
Synaptic plasticity via receptor tyrosine kinase/G protein-coupled receptor crosstalk
Authors: Lao-Peregrin Cristina , Xiang Guoqing , Kim Jihye , Srivastava Ipsit , Fall Alexandra B. , Gerhard Danielle M. , Kohtala Piia , Kim Daegeon , Song Minseok , Garcia-Marcos Mikel , Levitz Joshua , Lee Francis S. 
Published: August 2023

SummaryCellular signaling involves a large repertoire of membrane receptors operating in overlapping spatiotemporal regimes and targeting many common intracellular effectors. However, both the molecular mechanisms and physiological roles of crosstalk between receptors, especially those from different superfamilies, are poorly understood. We find that the receptor tyrosine kinase (RTK), TrkB, and the G protein-coupled receptor (GPCR), metabotropic glutamate receptor 5 (mGluR5), together mediate a novel form of hippocampal synaptic plasticity in response to brain-derived neurotrophic factor (BDNF). Activated TrkB enhances constitutive mGluR5 activity to initiate a mode-switch that drives BDNF-dependent sustained, oscillatory Ca2+signaling and enhanced MAP kinase activation. This crosstalk is mediated, in part, by synergy between Gβγ, released by TrkB, and Gαq-GTP, released by mGluR5, to enable a previously unidentified form of physiologically relevant RTK/GPCR crosstalk.
Impact of resistance on therapeutic design: a Moran model of cancer growth
Authors: Lacy Mason S. , Jenner Adrianne L. 
Published: August 2023

AbstractResistance of cancers to treatments, such as chemotherapy, largely arise due to cell mutations. These mutations allow cells to resist apoptosis and inevitably lead to recurrence and often progression to more aggressive cancer forms. Sustained-low dose therapies are being considered as an alternative over maximum tolerated dose treatments, whereby a smaller drug dosage is given over a longer period of time. However, understanding the impact that the presence of treatment-resistant clones may have on these new treatment modalities is crucial to validating them as a therapeutic avenue. In this study, a Moran process is used to capture stochastic mutations arising in cancer cells, inferring treatment resistance. The model is used to predict the probability of cancer recurrence given varying treatment modalities. The simulations predict that sustained-low dose therapies would be virtually ineffective for a cancer with a non-negligible probability of developing a sub-clone with resistance tendencies. Furthermore, calibrating the model toin vivomeasurements for breast cancer treatment with Herceptin, the model suggests that standard treatment regimens are ineffective in this mouse model. Using a simple Moran model, it is possible to explore the likelihood of treatment success given a non-negligible probability of treatment resistant mutations and suggest more robust therapeutic schedules.
A RAB7A Phosphoswitch Coordinates Rubicon Homology Protein Regulation of PINK1/Parkin-Dependent Mitophagy
Authors: Tudorica Dan A. , Basak Bishal , Cordova Alexia Puerto , Khuu Grace , Rose Kevin , Lazarou Michael , Holzbaur Erika L. F. , Hurley James H. 
Published: August 2023

SUMMARYActivation of PINK1 and Parkin in response to mitochondrial damage initiates a cytoprotective mitophagy response that includes phosphorylation of RAB7A at Ser72. Rubicon is a RAB7A binding protein that acts as a negative regulator of autophagy. The structure of the Rubicon-RAB7A complex suggests that phosphorylation of RAB7A at Ser72 would block Rubicon binding. Indeed,in vitrophosphorylation of RAB7A by TBK1 abrogates Rubicon-RAB7A binding. Pacer, a positive regulator of autophagy, has an RH domain with a basic triad predicted to bind an introduced phosphate. Consistent with this, Pacer-RH binds to phosho-RAB7A but not to unphosphorylated RAB7A. In cells, mitochondrial depolarization reduces Rubicon:RAB7A colocalization whilst recruiting Pacer to phospho-RAB7A-positive puncta. Pacer knockout reduces Parkin mitophagy with little effect on bulk autophagy or Parkin-independent mitophagy. Rescue of Parkin-dependent mitophagy requires the intact pRAB7A phosphate-binding basic triad of Pacer. Together these structural and functional data support a model in which the TBK1-dependent phosphorylation of RAB7A serves as a switch, promoting mitophagy by relieving Rubicon inhibition and favoring Pacer activation.
Huntingtin bundles and changes the local proteome of actin filaments in neurons
Authors: Carpentier Rémi , Capizzi Mariacristina , Kim Hyeongju , Novion-Ducassou Julia , Denarier Eric , Blot Béatrice , Couté Yohann , Arnal Isabelle , Song Ji-Joon , Humbert Sandrine 
Published: August 2023

AbstractHuntingtin (HTT) is a large protein whose best-known function being the facilitation of intracellular dynamics along the microtubule network by scaffolding molecular motors complexes. Our recent finding that the defective axonal growth in HD was due to altered growth cone architecture led us to ask whether HTT also influences the cytoskeleton itself. In developing neurons, we found that a large proportion of HTT associates with F-actin in growth cones. Using cell free system and purified recombinant proteins, we observed that HTT binds directly filamentous actin (F-actin) and organizes filaments into bundles. Transmission electron microscopy shows that HTT dimers crosslink adjacent filaments 20 nm apart. We also provide evidence that HTT binding on F-actin modulates the association of other proteins to this cytoskeleton. Notably, HTT limits the association of the growth cone protein Drebrin1 with F-actin. HTT depletion leads to abnormal cytoskeletal organization, localization of Drebrin1 in growth cones, and axonal growth. HTT therefore serves a scaffolding function for the cytoskeleton itself, what might be relevant for HD pathophysiology.
Accounting for Indigenous cultural connections to land: insights from two Indigenous groups of Australia
Authors: Finau Glenn , Jarvis Diane , Stoeckl Natalie , Larson Silva , Grainger Daniel , Douglas Michael , Ewamian Aboriginal Corporation  , Barrowei Ryan , Coleman Bessie , Groves David , Hunter Joshua , Lee Maria , Markham Michael 
Published: August 2023

PurposeThis paper aims to present the findings of a government-initiated project that sought to explore the possibility of incorporating cultural connections to land within the federal national accounting system using the United Nations Systems of Environmental-Economic Accounting (UN-SEEA) framework as a basis.Design/methodology/approachAdopting a critical dialogic approach and responding to the calls for critical accountants to engage with stakeholders, the authors worked with two Indigenous groups of Australia to develop a system of accounts that incorporates their cultural connections to “Country”. The two groups were clans from the Mungguy Country in the Kakadu region of Northern Territory and the Ewamian Aboriginal Corporation of Northern Queensland. Conducting two-day workshops on separate occasions with both groups, the authors attempted to meld the Indigenous worldviews with the worldviews embodied within national accounting systems and the UN-SEEA framework.FindingsThe models developed highlight significant differences between the ontological foundations of Indigenous and Western-worldviews and the authors reflect on the tensions created between these competing worldviews. The authors also offer pragmatic solutions that could be implemented by the Indigenous Traditional Owners and the government in terms of developing such an accounting system that incorporates connections to Country.Originality/valueThe paper contributes to providing a contemporary case study of engagement with Indigenous peoples in the co-development of a system of accounting for and by Indigenous peoples; it also contributes to the ongoing debate on bridging the divide between critique and praxis; and finally, the paper delves into an area that is largely unexplored within accounting research which is national accounting.
Exploring the effect of supervisor bottom-line mentality on subordinate work well-being: a self-determination theory perspective
Authors: Zhao Nan , He Bin , Sun Xu , Hu Weimin 
Published: August 2023

Purpose
This study aims to investigate the effect of supervisor bottom-line mentality (SBLM) on subordinate work well-being using self-determination theory. Furthermore, it examines the mediating role of subordinate harmonious work passion (HWP) and obsessive work passion (OWP), as well as the moderating role of subordinate family motivation on the indirect effect of SBLM on subordinate work well-being.

Design/methodology/approach
The authors conducted two studies, an experiment and a field study, to test the hypotheses. In Study 1, the authors conducted an experimental study using a sample of 127 undergraduate students to examine how family motivation moderates the relationship between SBLM and subordinate work passion. Concurrently, in Study 2, the authors conducted a time-lagged field study involving 261 corporate employees in China to validate the findings derived from Study 1, as well as test the entire conceptual model.

Findings
The authors find in Study 1 that family motivation moderates the effects of SBLM on subordinate HWP and OWP. Nevertheless, Study 2 uncovers a negative association between SBLM and subordinate work well-being, with HWP and OWP mediating this relationship. Besides, family motivation moderates the mediating effect of HWP on the relationship between SBLM and subordinate work well-being.

Originality/value
The main contribution of this study is that the negative effect of SBLM impacts subordinate work well-being, thereby building an accurate and fine-grained knowledge base of the detrimental effects of bottom-line mentality (BLM). Additionally, this study expands the frontiers of knowledge in this area by investigating the mediating mechanisms and boundary conditions of SBLM on subordinate work well-being, effectively addressing a theoretical gap in BLM research.
Academic libraries, ICT use and sustainable higher education development
Authors: Adekoya Clement Ola , Fasae Joseph Kehinde , Alade Adesola Victoria 
Published: August 2023

Purpose
Education is a strong pillar to national development. It is vital to ensure sustainable higher education development (SHED) in a bid to facilitate global development. This study aims to investigate academic libraries, information and communication technology (ICT) use and SHED.

Design/methodology/approach
Descriptive survey research design was used for this study. The population of the study was 607 librarians in the 41 public university libraries in Southern Nigeria. Total enumeration and purposive sampling techniques were used for this study. The instrument of data collection was questionnaire. Descriptive statistics was used for the data analysis.

Findings
It was found that qualified teachers and promotion of scholarship constitute SHED. The extent of contribution of academic libraries to SHED is high. Academic libraries face a number of challenges in their effort to provide information services for the actualisation of the SHED. The extent of use of ICT in SHED is high. It was therefore recommended that academic libraries and ICT should be adequately used to accomplish SHED in Nigeria.

Research limitations/implications
This study will contribute to the body of literature on how academic libraries and the use of ICT can contribute to SHED.

Practical implications
While SHED is central to global development, the role of academic libraries and the use of ICT should be unanimously embraced by all the stakeholders in education industry.

Originality/value
SHED is inevitable in the contemporary era. This study reveals that academic libraries and ICT use are vital in achieving SHED.