bluuebunny/crossref_metadata_2025_split huggingface dataset:
ATP13A2 activates the pentose phosphate pathway to promote colorectal cancer growth though TFEB‐PGD axis
Authors: Zhang Fan , Wu Zhiwei , Yu Bowen , Ning Zhengping , Lu Zhixing , Li Liang , Long Fei , Hu Qionggui , Zhong Chonglei , Zhang Yi , Lin Changwei 
Published: May 2023

AbstractBackgroundThe pentose phosphate pathway (PPP) is an important mechanism by which tumour cells resist stressful environments and maintain malignant proliferation. However, the mechanism by which the PPP regulates these processes in colorectal cancer (CRC) remains elusive.MethodsClosely related PPP genes were obtained from the TCGA and GEO databases. The effect of ATP13A2 on CRC cell proliferation was evaluated by performing in vitro assays. The connection between the PPP and ATP13A2 was explored by assessing proliferation and antioxidative stress. The molecular mechanism by which ATP13A2 regulates the PPP was investigated using chromatin immunoprecipitation and dual luciferase experiments. The clinical therapeutic potential of ATP13A2 was explored using patient‐derived xenograft (PDX), patient‐derived organoid (PDO) and AOM/DSS models.FindingsWe identified ATP13A2 as a novel PPP‐related gene. ATP13A2 deficiency inhibited CRC growth and PPP activity, as manifested by a decrease in the levels of PPP products and an increase in reactive oxygen species levels, whereas ATP13A2 overexpression induced the opposite effect. Mechanistically, ATP13A2 regulated the PPP mainly by affecting phosphogluconate dehydrogenase (PGD) mRNA expression. Subsequent studies showed that ATP13A2 overexpression promoted TFEB nuclear localization by inhibiting the phosphorylation of TFEB, thereby enhancing the transcription of PGD and ultimately affecting the activity of the PPP. Finally, ATP13A2 knockdown inhibited CRC growth in PDO and PDX models. ATP13A2−/− mice had a lower CRC growth capacity than ATP13A2+/+ in the AOM/DSS model.Our findings revealed that ATP13A2 overexpression‐driven dephosphorylation of TFEB promotes PPP activation by increasing PGD transcription, suggesting that ATP13A2 may serve as a potential target for CRC therapy.
Prevention of foot ulcers in persons with diabetes at risk of ulceration: A systematic review and meta‐analysis
Authors: van Netten Jaap J. , Raspovic Anita , Lavery Lawrence A. , Monteiro‐Soares Matilde , Paton Joanne , Rasmussen Anne , Sacco Isabel C. N. , Bus Sicco A. 
Published: May 2023

AbstractAimsPrevention of foot ulcers in persons with diabetes is important to help reduce the substantial burden on both individual and health resources. A comprehensive analysis of reported interventions is needed to better inform healthcare professionals about effective prevention. The aim of this systematic review and meta‐analysis is to assess the effectiveness of interventions to prevent foot ulcers in persons with diabetes who are at risk thereof.Materials and MethodsWe searched the available scientific literature in PubMed, EMBASE, CINAHL, Cochrane databases and trial registries for original research studies on preventative interventions. Both controlled and non‐controlled studies were eligible for selection. Two independent reviewers assessed risk of bias of controlled studies and extracted data. A meta‐analysis (using Mantel‐Haenszel's statistical method and random effect models) was done when >1 RCT was available that met our criteria. Evidence statements, including the certainty of evidence, were formulated according to GRADE.ResultsFrom the 19,349 records screened, 40 controlled studies (of which 33 were Randomised Controlled Trials [RCTs]) and 103 non‐controlled studies were included. We found moderate certainty evidence that temperature monitoring (5 RCTs; risk ratio [RR]: 0.51; 95% CI: 0.31–0.84) and pressure‐optimised therapeutic footwear or insoles (2 RCTs; RR: 0.62; 95% CI: 0.26–1.47) likely reduce the risk of plantar foot ulcer recurrence in people with diabetes at high risk. Further, we found low certainty evidence that structured education (5 RCTs; RR: 0.66; 95% CI: 0.37–1.19), therapeutic footwear (3 RCTs; RR: 0.53; 95% CI: 0.24–1.17), flexor tenotomy (1 RCT, 7 non‐controlled studies, no meta‐analysis), and integrated care (3 RCTs; RR: 0.78; 95% CI: 0.58–1.06) may reduce the risk of foot ulceration in people with diabetes at risk for foot ulceration.ConclusionsVarious interventions for persons with diabetes at risk for foot ulceration with evidence of effectiveness are available, including temperature monitoring (pressure‐optimised) therapeutic footwear, structured education, flexor tenotomy, and integrated foot care. With hardly any new intervention studies published in recent years, more effort to produce high‐quality RCTs is urgently needed to further improve the evidence base. This is especially relevant for educational and psychological interventions, for integrated care approaches for persons at high risk of ulceration, and for interventions specifically targeting persons at low‐to‐moderate risk of ulceration.
Practical guidelines on the prevention and management of diabetes‐related foot disease (IWGDF 2023 update)
Authors: Schaper Nicolaas C. , van Netten Jaap J. , Apelqvist Jan , Bus Sicco A. , Fitridge Robert , Game Fran , Monteiro‐Soares Matilde , Senneville Eric ,   the IWGDF Editorial Board
Published: May 2023

AbstractDiabetes‐related foot disease results in a major global burden for patients and the healthcare system. The International Working Group on the Diabetic Foot (IWGDF) has been producing evidence‐based guidelines on the prevention and management of diabetes‐related foot disease since 1999. In 2023, all IWGDF Guidelines have been updated based on systematic reviews of the literature and formulation of recommendations by multidisciplinary experts from all over the world. In addition, a new guideline on acute Charcot neuro‐osteoarthropathy was created. In this document, the IWGDF Practical Guidelines, we describe the basic principles of prevention, classification and management of diabetes‐related foot disease based on the seven IWGDF Guidelines. We also describe the organisational levels to successfully prevent and treat diabetes‐related foot disease according to these principles and provide addenda to assist with foot screening. The information in these practical guidelines is aimed at the global community of healthcare professionals who are involved in the care of persons with diabetes. Many studies around the world support our belief that implementing these prevention and management principles is associated with a decrease in the frequency of diabetes‐related lower‐extremity amputations. The burden of foot disease and amputations is increasing at a rapid rate, and comparatively more so in middle to lower income countries. These guidelines also assist in defining standards of prevention and care in these countries. In conclusion, we hope that these updated practical guidelines continue to serve as a reference document to aid healthcare providers in reducing the global burden of diabetes‐related foot disease.
Increasing complexity of opsin expression across stomatopod development
Authors: Palecanda Sitara , Steck Mireille , Porter Megan L. 
Published: May 2023

AbstractStomatopods are well studied for their unique visual systems, which can consist of up to 16 different photoreceptor types and 33 opsin proteins expressed in the adults of some species. The light‐sensing abilities of larval stomatopods are comparatively less well understood with limited information about the opsin repertoire of these early‐life stages. Early work has suggested that larval stomatopods may not possess the extensive light detection abilities found in their adult counterparts. However, recent studies have shown that these larvae may have more complex photosensory systems than previously thought. To examine this idea at the molecular level, we characterized the expression of putative light‐absorbing opsins across developmental stages, from embryo to adult, in the stomatopod species Pullosquilla thomassini using transcriptomic methods with a special focus on ecological and physiological transition periods. Opsin expression during the transition from the larval to the adult stage was further characterized in the species Gonodactylaceus falcatus. Opsin transcripts from short, middle, and long wavelength‐sensitive clades were found in both species, and analysis of spectral tuning sites suggested differences in absorbance within these clades. This is the first study to document the changes in opsin repertoire across development in stomatopods, providing novel evidence for light detection across the visual spectrum in larvae.
Long‐term opioid treatment and endocrine measures in chronic non‐cancer pain patients
Authors: Diasso Pernille D. K. , Abou‐Kassem Dalia , Nielsen Susanne D. , Main Katharina M. , Sjøgren Per , Kurita Geana P. 
Published: May 2023

AbstractBackgroundThe prevalence of chronic non‐cancer pain (CNCP) has increased dramatically the past decades, which combined with indiscriminate use of prescribed opioids has become a public health problem. Endocrine dysfunction may be a complication of long‐term opioid treatment (L‐TOT), but the evidence is limited. This study aimed at investigating the associations between L‐TOT and endocrine measures in CNCP patients.MethodsCortisol (spot and after stimulation), thyrotropin (TSH), thyroxin (T4), insulin‐like growth factor 1 (IGF‐1), prolactin (PRL), 17‐hydroxyprogesterone, androstenedione, dehydroepiandrosterone (DHEAS), sex hormone‐binding globulin (SHBG), total testosterone (TT) and free testosterone (fT) were measured. Group comparisons were done between CNCP patients in L‐TOT and controls as well as between patients on high‐ or low‐dose morphine equivalents.ResultsEighty‐two CNCP patients (38 in L‐TOT and 44 controls not receiving opioids) were included. Low TT (p = 0.004) and fT concentrations (p < 0.001), high SHBG (p = 0.042), low DEAS (p = 0.017) and low IGF‐1 (p = 0.003) in men were found when comparing those in L‐TOT to controls and high PRL (p = 0.018), low IGF‐1 standard deviation score (SDS) (p = 0.006) along with a lesser, but normal cortisol response to stimulation (p = 0.016; p = 0.012) were found when comparing L‐TOT to controls. Finally, a correlation between low IGF‐1 levels and high opioid dose was observed (p < 0.001).ConclusionsOur study not only supports previous findings but even more interestingly disclosed new associations. We recommend future studies to investigate endocrine effects of opioids in larger, longitudinal studies. In the meanwhile, we recommend monitoring endocrine function in CNCP patients when prescribing L‐TOT.SignificanceThis clinical study found associations between L‐TOT, androgens, growth hormone and prolactin in patients with CNCP compared to controls. The results support previous studies as well as add new knowledge to the field, including an association between high opioid dose and low growth hormone levels. Compared to existing research this study has strict inclusion/exclusion criteria, a fixed time period for blood sample collection, and adjustments for potential confounders, which has not been done before.
Dye adsorbed few‐layer thick Ti3C2Tx‐MXenes for direct electrochemical detection of CD44 proteins
Authors: Jawaid Sana , Hussain Tumrani Sadam , Nafady Ayman , Sirajuddin  , Karakuş Selcan , Tabish Mohammad , Ahmed Nazeer 
Published: May 2023

AbstractThe direct electrochemical detection of cancer biomarkers using single single‐component platforms is challenging. Herein, we propose constructing an efficient screen‐printed electrode (SPE) based platform for selective detection of CD44 proteins, a non‐kinase transmembrane glycoprotein. A sensing platform, MB‐MX/HA/SPE, was developed by incorporating few‐layered Ti3C2Tx nanosheets pre‐loaded with methylene blue (MB) dye. The nanosheets were subsequently immobilized with hyaluronic acid (HA), which served as a ligand for the specific recognition of CD44. The simple electrode configuration and the highly conductive Ti3C2Tx facilitated the electrochemical oxidation of MB, generating a reference SWV signal that declined proportionally with the increasing concentration of CD44 owing to ligand (HA)‐protein interaction. The sensor could register a sensitive inhibition response in the concentration range of 0.1 to 7.25 ng.mL−1 with a detection limit of 1.2×10−2 ng.mL−1 for CD44 proteins. Moreover, the synergistic combination of the highly conductive/adsorptive Ti3C2Tx nanosheets and hyaluronic acid (HA) led to strong antifouling characteristics even in the presence of other common proteins, such as bovine serum albumin (BSA), haemoglobin (Ig), immunoglobulin G (IgG), prostate‐specific antigen (PSA), and neuron‐specific enolase (NSE). The proposed strategy eliminates the need for additional components in the electrode modification procedure. In addition, incorporating MXenes as electrode material paves the way for developing sensitive biosensors with prospective applications in cancer diagnosis.
Hyper contour cryptography with master Birch‐Brown Paruvala‐SLSTM model for enhanced healthcare security
Authors: Angelin P. Blessed , Franklin S. Wilfred 
Published: May 2023

AbstractBlockchain is a reliable ledger database that may be used to store various private healthcare data, but uploading and sharing data is difficult due to privacy concerns. Hence, to overcome these issues, a novel HCCLO with master Birch‐Brown Paruvala‐SLSTM model has been proposed to store various patients' medical data and shield it from threats. Hyper Contour Cryptography with Lepidoptera Optimization (HCCLO) has been proposed in this model, which encrypts data to reduce unwanted time stamp creation and prevent private key theft. Also, the Master Birch‐Brown‐Parulava NN design is introduced to prevent a false identity that leads to a data breach, and it allows for private exchange and enhances framework reconciliation, acting as an immune response to information breaks in the chain and preventing data breaches from spreading further. Then, the SLSTM layer implant RF Classifier is introduced to categorize data as user requests and attacks, safeguarding the blocks against changeability caused by malware attacks. The proposed approach offers minimal block mine time, maximum accuracy, and healthcare data security.
Protective effects of Althaea officinalis L. extract against N‐diethylnitrosamine‐induced hepatocellular carcinoma in male Wistar rats through antioxidative, anti‐inflammatory, mitochondrial apoptosis and PI3K/Akt/mTOR signaling pathways
Authors: Wang Zhenqian , Jiang Xiao , Zhang Long , Chen Han 
Published: May 2023

AbstractHepatocellular carcinoma is the fourth cause of death due to cancer and includes 90% of liver tumors. Therefore, in this study, it was tried to show that Althaea officinalis L. flower extract (ALOF) can protect hepatocytes against N‐diethylnitrosamine (DEN)‐induced hepatocellular carcinoma. Totally, 70 Wistar rats were divided into seven groups (n = 10/group) of sham, DEN, treatment with silymarin (SIL; DEN + SIL), treatment with ALOF (DEN + 250 and 500 ALOF), and cotreatment with SIL and ALOF (DEN + SIL + 250 and 500 ALOF). At the end of the study, the serum levels of liver indices (albumin, total protein, bilirubin, C‐reactive protein, ALT, AST, and ALP), inflammatory cytokines (IL‐6, IL‐1β, IL‐10, and TNF‐α), and oxidants parameters (glutathione peroxidase [GPx], superoxide dismutase [SOD], catalase [CAT] activity along with nitric oxide [NO] levels) were evaluated. The level of Bax, Bcl‐2, Caspase‐3, p53, PI3K, mTOR, and AKT genes were measured. ALOF in cotreatment with SIL was able to regulate liver biochemical parameters, improve serum antioxidant indices, and decrease the level of proinflammatory cytokines significantly (p < .05). ALOF extract in both doses of 250 and 500 mg/kg in cotreatment with SIL caused a significant (p < .05) decrease in the p53‐positive cells and a significant (p < .05) increase in Bcl‐2‐positive cells. Therefore, ALOF was able to modulate the proliferation of cancer cells and protect normal cells through the regulation of Bax/Bcl‐2/p53 and PI3K/Akt/mTOR signaling pathways. It seems that ALOF can be used as a prodrug or complementary treatment in the protection of hepatocytes in induced damages caused by carcinogens.
Stock market liberalisation and acquisition outcomes: Evidence from a natural experiment
Authors: Lin Yanyan , Hou Xipei 
Published: May 2023

AbstractTaking the Shanghai/Shenzhen‐Hong Kong stock connect policy as a natural experiment, we use a difference‐in‐differences approach to investigate how stock market liberalisation affects M&A outcomes. Employing a sample of Chinese listed firms from 2011 to 2020, we find that the stock market liberalisation leads to more positive M&A announcement returns. The positive effect on M&A announcement returns is the result of increased investor sentiment after market liberalisation. It is the investor sentiment mechanism through which stock market liberalisation has effects on M&A short‐term returns. Further analysis indicates that stock market liberalisation does not impose positive effects on long‐term M&A outcomes. We do not find evidence that M&As initiated by connected firms to be more value‐added.
Reducing urinary tract infection in female pelvic surgery: A retrospective cohort study
Authors: Mann Gurkiran K. , Koenig Nicole A. , Lee Terry , Geoffrion Roxana 
Published: May 2023

AbstractObjectiveTo compare prebundle versus postbundle implementation urinary tract infection (UTI) rates among inpatients within 6 weeks of clean‐contaminated pelvic reconstructive surgery.MethodsThe authors conducted a retrospective cohort study from September 2019 to December 2021 at a tertiary hospital. The bundle strategy included the following: universal preoperative UTI check with treatment if positive, replacing prolonged postoperative voiding trials on the ward with earlier discharge and indwelling catheter removal by a nurse continence advisor the next day, and daily cranberry extract for 6 weeks postoperatively. UTI was defined as positive urine culture (≥100 000 colony‐forming unit per mL) in a symptomatic patient. Data analysis involved hypothesis testing and logistic regression.ResultsThe authors reviewed 132 postbundle inpatient charts and retained 93 for analyses. The results were compared with 204 prebundle inpatient charts. The rate of postoperative UTI decreased from 17.6% in the prebundle group to 6.5% after bundle implementation (P = 0.01). The adjusted odds ratio for postbundle versus prebundle likelihood of UTI was 0.35 (95% confidence interval, 0.13–0.98; P = 0.045). Significantly more postbundle patients compared with prebundle patients were discharged home on the first day postoperatively (76.3% vs. 37.7%, P < 0.001).ConclusionsA clinical bundle can significantly decrease both UTI rates and hospital stay after pelvic reconstructive surgery.
Coaxial microbial electrolysis cell for cost‐effective bioenergy production and wastewater treatment of potato industry effluent
Authors: Muddasar Muhammad , Liaquat Rabia , Rahman Muhammad Zia Ur , Khoja Asif Hussain , Aslam Ayesha , Basit Abdul 
Published: May 2023

AbstractBACKGROUNDThe increase in energy and water demand due to industrialization and urbanization requires prioritized solutions for a sustainable future. Microbial electrolysis cells (MECs) have shown huge potential for biohydrogen production along with wastewater treatment. This study examined the effectiveness of employing nickel foam as an anode material for biohydrogen production from the widely available potato industry effluent.RESULTSHydrogen production rate increases exponentially with the increase in applied voltage. A maximum hydrogen production rate of 0.69 ± 0.02 m3H2 m−3reactor volume d−1was achieved at 0.9 V with a maximum chemical oxygen demand removal efficiency of 97% at 0.8 V. The effluent of the 0.8 V cycle had the least salinity, low total dissolved solids, 84% reduced total hardness, highest effluent clarity (4.30 NTU, and negligible quantity of heavy metals.CONCLUSIONThis study successfully produced biohydrogen from potato wastewater within 5 days of operation along with wastewater treatment of the substrate. The improved performance of the system can be attributed to the unique characteristics of nickel foam, such as high porosity, large surface area and excellent conductivity. The findings of this study have implications for the sustainable treatment of domestic and agro‐industrial wastewater and the development of efficient and low‐cost bio‐electrochemical systems for renewable energy production. © 2023 Society of Chemical Industry (SCI).
Homologous recombination repair gene‐based risk model predicts prognosis and immune microenvironment for primary lung cancer after previous malignancies
Authors: Wang Haoran , Gong Fangxiao , Kong Weicheng , Chen Yanyan , Zhang Jie 
Published: May 2023

AbstractBackgroundHomologous recombination repair (HRR) plays an important role in cancer development, drug resistance, and immune escape, but the role of HRR genes in primary lung cancer (PLC) after previous malignancies is unclear.MethodsWe used HRR‐related score constrcted by HRR genes to classify patients into two groups and compared clinical progression, differential genes, and their functions between them. Then, we constructed a prognostic risk model based on HRR‐related score and screened key differentially expressed genes. We evaluated the potential roles, mutational information, and immune correlations of key genes. Finally, we compared the long‐term prognosis and immune correlations of different prognostic risk subgroups.ResultsWe found that HRR‐related score was associated with T‐stage, immunotherapy sensitivity, and prognosis of PLC after previous malignancies. Differential genes between HRR‐related low‐score and high‐score groups are mainly involved in DNA replication and repair processes, such as the cell cycle. We identified three key genes, ABO, SERPINE2, and MYC, by machine learning, and MYC had the highest amplification mutation frequency. We verified that the key gene‐based prognostic model can better assess the prognosis of patients. The risk score of the prognostic model was associated with immune microenvironment and efficacy of immunotherapy.ConclusionsOverall, we identified three key genes ABO, SERPINE2, and MYC associated with HRR status in PLC after previous malignancies. The risk model based on key genes is associated with immune microenvironment and can well predict the prognosis for PLC after previous malignancies.
HTLV‐1 cell‐free DNA in plasma as a potential biomarker in HTLV‐1 carriers and adult T‐cell leukemia‐lymphoma
Authors: Katsuya Hiroo , Nakamura Hideaki , Maeda Aya , Ishii Keitaro , Nagaie Toshiaki , Sano Haruhiko , Sano Haruna , Itamura Hidekazu , Okamoto Sho , Ando Toshihiko , Watanabe Toshiki , Uchimaru Kaoru , Satou Yorifumi , Sueoka Eisaburo , Kimura Shinya 
Published: May 2023

AbstractViral cell‐free DNA (cfDNA) in plasma has been widely evaluated for detecting cancer and monitoring disease in virus‐associated tumors. We investigated whether the amount of cfDNA of human T‐cell leukemia virus type 1 (HTLV‐1) correlates with disease state in adult T‐cell leukemia‐lymphoma (ATL). HTLV‐1 cfDNA in aggressive ATL was significantly higher than that in indolent ATL and asymptomatic carriers. Notably, patients with lymphoma type represented higher HTLV‐1 cfDNA amount than chronic and smoldering subtypes, though they had no abnormal lymphocytes in the peripheral blood. HTLV‐1 cfDNA can be a universal biomarker that reflects the expansion of ATL clones.