bluuebunny/crossref_metadata_2025_split huggingface dataset:
Three-dimensional oropharyngeal airway changes in adolescent patients treated with extractions: a preliminary study
Authors: Sun Jianwei , Qi Yizhe , Song Baolong , Li Yongming 
Published: December 2022

Abstract
Objectives: To evaluate three-dimensional oropharyngeal airway changes after the extraction treatment in adolescents.
Methods: Forty class I adolescents were selected, treated with extraction of four first premolars with non-maximum anchorage. The upper airway was reconstructed and segmented with Dolphin 11.8. The changes of oropharyngeal airway volume and cross-sectional area were measured. The measurements were analyzed with SPSS 26.0.
Results: Compared with pre-treatment, the palatopharyngeal volume, glossopharyngeal volume, total oropharyngeal volume and minimum cross-sectional area increased significantly (P < 0.05), and the minimum cross-sectional area of oropharynx was mostly located in the glossopharyngeal region. The cross-sectional area and the maximum anterior and posterior diameter of the uvula tip decreased significantly. Univariate correlation analysis showed that there was significant difference after the retraction of upper and lower central incisors (P < 0.05). The results of multivariate regression analysis showed that the retraction (OR=1.821,95% CI: -1.554~-0.060, P=0.042) of the mandibular central incisor was an independent risk factor for the decrease of the cross-sectional area of the uvula tip after tooth extraction.
Conclusions: With non-maximum anchorage, the effect of extractions on the oropharyngeal airway of skeletal class Ⅰ adolescents is generally small, but it will change the shape and is related to the retraction of anterior teeth.
Quantitative Multiplex Real-Time Polymerase Chain Reaction Assay for the Detection of Helicobacter pylori and Clarithromycin Resistance
Authors: Kim Ilsoo , Maeng Lee-So , Kim Joon Sung , Kim Byung-Wook , Cheung Dae Young , Kim Jin Il , Park Soo-heon 
Published: December 2022

Abstract
Background
Identifying clarithromycin resistance is essential for eradicating Helicobacter pylori (HP). Therefore, we evaluated the performance of Allplex™ H.pylori & ClariR Assay (Allplex™) for diagnosing and detecting clarithromycin resistance in HP.
Methods
Subjects who underwent esophagogastroduodenoscopy between April 2020 and August 2021 at Incheon St. Mary’s hospital were enrolled in this study. The diagnostic performances of Allplex™ and dual priming oligonucleotide (DPO)-based multiplex polymerase chain reaction (PCR) were compared with sequencing as the gold standard.
Results
A total of 142 gastric biopsy samples were analyzed. Gene sequencing revealed 124 HP infections, 42 A2143G mutations, 2 A2142G mutations, one dual mutation, and no A2142C mutation. DPO-PCR showed 96.0% sensitivity and 100.0% specificity for HP detection; the corresponding rates for Allplex™ were 99.2% and 100.0%. DPO-PCR showed 88.3% sensitivity and 82.0% specificity for A2143G mutation, and Allplex™ showed 97.6% and 96.0%. The Cohen’s Kappa coefficient for overall test results was 0.56 for DPO-PCR and 0.95 for Allplex™.
Conclusion
Allplex™ showed comparable diagnostic performance with direct gene sequencing and non-inferior diagnostic performance to DPO-PCR. Further research is required to confirm whether Allplex™ is an effective diagnostic tool for the eradication of HP
Decomposition of unpolarized fluorescence spectrum of uniaxially oriented 1,3,5-triphenylbenzene microcrystals into polarized fluorescence spectra
Authors: Hara Michihiro , Takeshita Tatsuya , Kurata Hiroyuki , Kimura Tsunehisa 
Published: December 2022

Abstract
Luminescence from solids such as crystals and aggregates is of growing academic and industrial interest. In this study, we report decomposition of the unpolarized fluorescence spectrum of uniaxially oriented 1,3,5-triphenylbenzene (TPB) microcrystals into four polarized spectra measured with polarizer (V: vertical and H: horizontal) and analyser (V: vertical and H: horizontal), where V and H indicate perpendicular and parallel to the layer of TPB molecules in the crystal, respectively. Resolved spectra were interpreted in terms of the molecular and excimer like (J- and H-dimer) emissions. The origin of the excimer like emissions was discussed in relation to the molecular packing in the crystal. It was shown that polarized crystal fluorescence can provide insight into the excitation/emission process in the crystal. Although preliminary, this study demonstrates the potential of polarized fluorescence to elucidate the luminescent mechanism.
Phylogenetic and morphological re-evaluation of Antherospora on Barnardia japonica collected in Japan
Authors: Hashimoto Akira , Shibata Saho , Ohkuma Moriya 
Published: December 2022

Abstract
Antherospora vaillantii is an anther smut fungus that was originally considered occurring on Muscari spp. around the world; however, cryptic species were revealed during recent taxonomic investigations, thus emphasizing the importance of host specificity. In Japan, A. vaillantii was identified on Barnardia japonica in 1936. Although the recent revelations indicate that it may be part of a complex based on the host plant genus, the phylogenetic placement of this Japanese species is currently unresolved. In this investigation, seven specimens of the A. vaillantii complex were collected from the Kanto area of Japan, and six new single teliospore isolates were established. Molecular phylogenetic analyses based on the internal transcribed spacer sequences and large subunit nuclear ribosomal DNA (28S) region indicated that the new samples formed a monophyletic clade with other Antherospora spp. among Floromycetaceae lineage. Our samples were separated from other known species of Antherospora and formed a robust monophyletic clade in any analysis. Detailed morphological observations revealed that the new samples could be distinguished from other known Antherospora species. As a result, it is proposed that A. barnardiae can accommodate B. japonica parasites. The evolutionary processes of Floromycetaceae in relation to their Asparagaceous host plants have also been carefully discussed. Based on the morphological observations of the basidiospore ontogenic patterns, the familial concept of Floromycetaceae was redefined.
Mechanical performance and thermal stability of hardened portland cement -recycled sludge pastes containing MnFe 2 O 4 nanoparticles
Authors: Mohamed O. A. , El-dek S. I. , El-Gamal S. M.A. 
Published: December 2022

Abstract

Recently, recycling some industrial wastes becomes an essential way of the crucial challenges that mitigate their risks in the future. This work focused on utilization of recycled alum sludge (RAS) for partial replacing of Ordinary Portland cement (OPC), to contribute to solving the problems encountered by cement production as well as stockpiling of large quantities of water treated sludge waste, and to elaborate the particularities of different OPC-RAS blends by using MnFe2O4 spinel nanoparticles (NMFs). The outcomes of compressive strength, bulk density, water absorption, and stability again firing tests fastened the suitability of utilization of RAS waste for replacing OPC (maximum limit 10%) to prepare building material having enhanced strength and toughness. Inclusion of NMFs nanoparticles (0.5 mass%) in OPC pastes superseded with different quantities of RAS waste, motivates the configuration of hardened nanocomposites with improved physico-mechanical characteristics and stability against firing. Composite having 90% OPC-10% RAS – 0.5% NMFs represented the optimal choice for general construction application. TG/DTG, XRD, and SEM techniques affirmed the activity of NMFs particles, as they demonstrated the formation of enormous phases (as MH, CAHs, MnCSH, CSHs, CASHs, and CFH), especially 0.5% addition. These products boosted the robustness and degradation resistance of the nanocomposites upon firing.
Long non-coding RNA SOX2OT enhances cancer biological traits via sponging to tumor suppressor miR-122-3p and miR-194-5p in non- small cell lung carcinoma
Authors: Dodangeh Fatemeh , Sadeghi Zahra , Maleki Parichehr , Raheb Jamshid 
Published: December 2022

Abstract
The oncogenic role of long non-coding RNA SOX2 overlapping transcript (SOX2-OT) has been demonstrated as a miRNA decay system that sponges to tumor suppressor miRNA including miR-122-3p in glioblastoma and miR-194-5p in glioblastoma, gastric and colorectal cancers. Although, the molecular function of SOX2-OT is still unknown in most cancer including lung cancer. As aim of current study, we evaluated downstream regulation function of SOX2-OT in A549 and Calu-3 lung cancer cell lines. We knock down SOX2-OT expression with using of RNA interference system that had significant decreased expression in A549 and Calu-3 cells. Then, expression of down-regulating miRNAs (miR-122-3p and miR-194-5p) evaluated that showed increased expression of miR-122-3p and miR-194-5p. Also, expression of miRNAs downstream mRNA including FOXO1 (Forkhead Box O1) and FOXA1 (Forkhead Box O1), changed. Recently, Critical roles of FOXO1 and FOXA1 proteins in pathways that involved in proliferation, metastasis and apoptosis has been demonstrated. Downstream changes in cellular traits, as evaluated by MTT, flow cytometry, metastasis and apoptosis assays proved that biological behaviors of lung cancer cells influenced after SOX2-OT knockdown. Overall, results of current study bring up oncogenic role for SOX2-OT via regulation of miR-122-3p/FOXO1 and miR-194-5p/FOXA1 axes.
Analysis of the mechanism of hyperosmotic tolerance in Gracilariopsis lemaneiformis based on WGCNA
Authors: Xiao Baoheng , Feng Xiaoqing , Li Pingping , Sui Zhenghong 
Published: December 2022

Abstract
The salt-tolerant mutants HAGL-X5, HAGL-X3, and the control were subjected to transcriptome assays after three weeks of treatment with artificial seawater of different salinities (30‰, 45‰ and 60‰). A large number of differential genes were obtained in the transcriptome, and we performed weighted co-expression network analysis (WGCNA) of these differential genes. The results showed that the samples that correlated well with salinity traits were mainly Ctr-60, X3-60 and X5-60, while the samples that correlated well with strain traits were mainly X5-30, X5-45 and X5-60. Then the modules that correlated well with salinity traits were mainly blue, red and tan modules. The modules that correlated well with strain traits were black, cyan, lightcyan, and yellows modules. KEGG enrichment of genes from the above modules revealed that the most significant enrichment pathways associated with salinity traits were the proteasome and carbon fixation by photosynthesis. The most significant enrichment pathways associated with strain traits were lipid metabolism, oxidative phosphorylation, and porphyrin and chlorophyll metabolism. From the expression analysis, the gene expression on the proteasome pathway and the carbon fixation pathway of photosynthesis was higher in all strains at a salinity of 60‰ than in all strains at other salinities. Also the gene expression of X5-60 was higher than that of Ctr-60 and X3-60 on the proteasome pathway. The expression of X5-30 genes was lower than that of Ctr-30 and X3-30 in oxidative phosphorylation as well as porphyrin and chlorophyll metabolism in strain-related enrichment pathways, where the low expression of genes of porphyrin and chlorophyll in X5-30 may be related to the higher NPQ value of X5-30. Finally, correlation analysis identified candidate genes with well correlation with salinity in proteasome and carbon fixation by photosynthesis, and with strain traits in lipid metabolism, oxidative phosphorylation, and porphyrin and chlorophyll metabolism.
Globally elevated levels of histone H3 lysine 9 trimethylation in early infancy are associated with poor growth trajectory in Bangladeshi children
Authors: Kupkova Kristyna , Shetty Savera J. , Haque Rashidul , Petri William A. , Auble David T. 
Published: December 2022

Abstract
Background
Stunting is a global health problem affecting hundreds of millions of children worldwide and contributing to 45% of deaths in children under the age of five. Current therapeutic interventions have limited efficacy. Understanding the epigenetic changes underlying stunting will elucidate molecular mechanisms and likely lead to new therapies.
Results
We profiled the repressive mark histone H3 lysine 9 trimethylation (H3K9me3) genome-wide in peripheral blood mononuclear cells (PBMCs) from 18-week-old infants (n = 15) and mothers (n = 14) enrolled in the PROVIDE study established in an urban slum in Bangladesh. We associated H3K9me3 levels within individual loci as well as genome-wide with anthropometric measurements and other biomarkers of stunting and performed functional annotation of differentially affected regions. Globally elevated H3K9me3 levels were associated with poor linear growth between birth and one year of age. A large proportion of the affected genes code for proteins targeting viral mRNA and highly significant regions were enriched in transposon elements with potential regulatory roles in immune system activation and cytokine production. Maternal data show a similar trend with child’s anthropometry, however, lack statistical significance to infer an intergenerational relationship.
Conclusions
We speculate that high H3K9me3 levels may result in poor linear growth by repressing genes involved in immune system activation. Importantly, changes to H3K9me3 were detectable before the overt manifestation of stunting and therefore may be valuable as new biomarkers of stunting.
Effect of Environmental Psychological Factors on Compliance with Brace Treatment in Adolescent Idiopathic Scoliosis
Authors: Li Kepeng , Han Ye 
Published: December 2022

Abstract
Objective

To investigate the Effect of Environmental Psychological Factors on Orthosis Therapy for Adolescent Idiopathic Scoliosis (AIS) in the three-dimensional perspectives included oneself, family and school environment.

Methods

Patients with AIS undergoing bracing therapy from January 2019 to December 2021 were retrospectively studied. Information about the patients included baseline data, Eysenck Personality Questionnaire, family environment scale, and academic achievement was collected. The patient‘s data of Eysenck Personality Questionnaire and family environment scale was compared with the Chinese norm to determine the representativeness of the included sample. The patients were divided into complianced group and control group according to the compliance condition, single factor analysis was carried out by χ2 test or independent sample t test. The meaningful variables were further analyzed by multivariate logistic regression.

Results

A total of 50 patients were included. There was no statistical difference observed in patient’s statistics compared with Chinese norm (P < 0.05). Univariate analysis results showed statistical difference in emotional stability (t = 3.14, P = 0.00) of the Eysenck Personality Questionnaire, whereas there was no statistical difference in internal and external direction (t = 1.07, P = 0.28), mental quality (t = 0.17, P = 0.86); There had statistical difference in intimacy (t = 3.96, P = 0.00) in the household environmental scale, whereas contradictions (t = 1.53, P = 0.13) and culture (t = 0.38, P = 0.70) were not statistically different; Statistical difference was observed in academic performance (χ2 = 7.96, P = 0.00). In the multivariate logistic regression analysis, Statistical difference was not observed in the emotional stability and academic achievement (p > 0.05). Intimacy remained an independent influence factor for compliance (P = 0.01, Exp (B) = 3.10, 95% confidence interval: 1.27～7.56).

Conclusion

High-intimacy family environment is an important environmental characteristic of high compliance. Effective communication between parents and patients is required in Orthosis Therapy for AIS.
The usefulness of Unmanned Aerial Vehicles (UAV) in white stork censusing
Authors: Tobółka Marcin , Aguirre José I. , Dylewski Łukasz , López-García Alejandro , Martínez Rodrigo Gimeno , Zbyryt Adam 
Published: December 2022

AbstractLong-term bird monitoring brings vital information on the effects of environmental changes on wildlife. However, covering a large area with direct observations in the field is time-consuming and economically costly. New technologies, such as Unmanned Aerial Vehicles (UAV), are effective and often noninvasive tools successfully used in bird monitoring. However, the stability of the method is essential when handling long-term data in the context of population changes. We examined the efficiency and precision of data collected by UAV and human observers within two distinct populations of the white storkCiconia ciconia, in Poland and Spain, presenting two different nesting patterns, solitary and colonial breeding. In Polish and Spanish populations, the number of fledglings was significantly lower when recorded by human observer than by UAV, i.e. 2.21 vs 2.60, and 1.35 vs 1.55. The mean time needed to record the number of fledglings was significantly longer when using a UAV than by a human observer. The number of detected nests in colonies differed significantly between the human observer and UAV, on average 13.1 vs 7.4, respectively. The difference number of recorded nests was linked with the type of nest substrates, i.e. on trees, the error was higher than in colonies located on human-made structures. The probability of mistake by the observer was significantly lower when recording the number of fledglings in colonial white storks in Spain than in solitary nesting in Poland. Although UAVs are a helpful tool in bird monitoring, in long-term studies, they must be used with caution and awareness that obtained results might differ from those obtained previously in a long-term monitoring framework.
Building Relationships to Connect Cancer Researchers with Community Members: ‘Bench to Community Pipeline’
Authors: Dahlke Allison R. , LoConte Noelle K. , Flickinger Angela , Richie Sara , Klink Jenna , Trentham-Dietz Amy , Shirley Rebecca , Litzelman Kristin 
Published: December 2022

Abstract
Purpose
Partnerships between researchers and community members and organizations can offer multiple benefits for research relevance and dissemination. The goal of this project was to build infrastructure to create bidirectional relationships between University of Wisconsin Carbone Cancer Center (UWCCC) researchers and community educators in the Division of Extension, which connects the knowledge and resources of the university to communities across the state.
Methods
This project had three aims: 1) create linkages with Extension; 2) establish an in-reach program to educate and train researchers on the science of Community Outreach and Engagement (COE); and 3) identify and facilitate collaborative projects between scientists and communities. Survey and focus group-based needs assessments were completed with both researchers and Extension educators and program activity evaluations were conducted.
Results
Most Extension educators (71%) indicated a strong interest in partnering on COE projects. UWCCC faculty indicated interest in further disseminating their research, but also indicated barriers in connecting with communities. Outreach webinars were created and disseminated to community, a “COE in-reach toolkit” for faculty was created and a series of “speed networking” events were hosted to pair researchers and community. Evaluations indicated the acceptability and usefulness of these activities and supported continuation of collaborative efforts.
Conclusion
Continued relationship and skill building, along with a sustainability plan, is critical to support the translation of basic, clinical and population research to action in the community outreach and engagement context. Further incentives for faculty should be explored for the recruitment of basic scientists into community engagement work.
Detection of gender-based violence in primary care in Central Catalonia: a descriptive cross-sectional study.
Authors: Muñoz-Sellés Ester , Pujolar-Díaz Georgina , Fuster-Casanovas Aïna , Catalina Queralt Miró 
Published: December 2022

Abstract
Background
Violence against women is a serious public health problem. Primary care could be one of the ideal places for the detection of gender-based violence, since women come into contact with PC at some point in their lives to look after their sexual and reproductive health.
The increase in initiatives promoted by the health authorities regarding gender-based violence offers the possibility of observing its evolution over the last few years.
Methods
A descriptive cross-sectional study of reported cases of gender-based violence in the region of Central Catalonia, during the period from 2017 to 2021, was carried out. All women of legal age, belonging to the specified health region and suffering episodes of gender-based violence, were included. The variables analysed were age, area of residence, health diagnoses related to gender-based violence, whether or not they were pregnant at the time of the attack, and mental health history.
Results
We found an increase in the detection of cases, although it must be noted that there is an underreporting of cases in PC. The prevalence according to the total number of women assigned per year over the period studied was 0.42% in 2017 and 0.48% in 2021. It has also been observed that the average number of episodes per woman increased from 1.03 in 2017 to 1.15 in 2021. During the 5 years analysed, the minimum number of episodes per woman was 1 and the maximum was 10. In reference to the duration of the episodes, the minimum was 1 day, and the maximum was 32 years.
Conclusions
Despite the established protocols and procedures, it seems that primary health care is not the most frequent place for its detection. It is necessary to continue working to raise awareness and train professionals, and to ensure coordination among all the parties involved in accompanying women in these processes.
Trial registration
CEIm: 21/278-P
Thin-shell wormhole under non-commutative geometry inspired Einstein-Gauss-Bonnet gravity
Authors: Rahman Nilofar , Kalam Mehedi , Das Amit , Islam Sayeedul , Rahaman Farook , Murshid Masum 
Published: December 2022

Abstract
Einstein-Gauss-Bonnet gravity is a generalization of the general relativity to higher dimensions in which the ﬁrst and second-order terms correspond to general relativity and Einstein-Gauss-Bonnet gravity respectively. We construct a new class of ﬁve-dimensional (5D) thin-shell wormholes by the `Cut-Paste' technique from black holes in Einstein-Gauss-Bonnet gravity inspired by non-commutative geometry starting with a static spherically symmetric, Gaussian mass distribution as a source and for this structural form of the thin shell wormhole we have explored several salient features of the solution, viz., pressure-density profile, equation of state, the nature of wormhole, total amount of exotic matter content at the shell. We have also analyzed the linearized stability of the constructed wormhole. From our study, we can assert that our model is found to be plausible with reference to the other model of thin-shell wormhole available in the literature.