bluuebunny/crossref_metadata_2025_split huggingface dataset:
Artemisia Absinthium Extract Attenuates the Quinolinic Acid-Induced
Cell Injury in OLN-93 Cells
Authors: Rashidi Roghayeh , Akaberi Maryam , Gholoobi Aida , Ghazavi Hamed , Forouzanfar Fatemeh 
Published: March 2023

Objective:
Increased quinolinic acid (QA) accumulation has been found in many neurodegenerative
diseases. Artemisia absinthium (A. absinthium) has been reported to have neuroprotective
and antioxidant activities. This study was designed to evaluate the effect of A. absinthium in QAinduced
neurotoxicity in OLN-93 Cells.

Methods:
OLN-93 cells were cultured in a DMEM medium containing 10% (v/v) fetal bovine serum,
100 units/ml penicillin, and 100 μg/ml streptomycin. The cells were pretreated with concentrations of
A. absinthium extract for two h and then exposed to QA for 24 h. After 24 h cell viability, the level of
malondialdehyde (MDA), reactive oxygen species (ROS), and apoptotic cells were quantitated in
OLN-93 Cells.

Results:
Pretreatment with A. absinthium extract prevented the loss of cell viability in OLN-93 cells.
ROS generation, lipid peroxidation, and apoptosis in QA -injured OLN-93 cells were reduced following
A. absinthium extract pretreatment.

Conclusion:
A. absinthium extract exerts its neuroprotective effect against QA-induced neurotoxicity
via oxidative stress and apoptosis modulation.
Polyacrylic Acid Supported L-proline as an Effective Heterogeneous
Catalyst for the Direct Asymmetric Aldol Reaction
Authors: Zhang Cuizhi , Fan Yanchao , Wang Sijia , Liu Shaojie , Chu Xiaomeng , Tang Erjun 
Published: March 2023

Introduction:
L-proline is an efficient chiral small-molecule organocatalyst for the direct asymmetric aldol reaction between unmodified acetone and a variety of aldehydes.

Methods:
However, it is difficult to separate from the reaction medium for reuse. In this work, poly-acrylic acid (PAA) supported The acylation reaction between L-hydroxyproline and PAA prepared l-proline (P(AA-co-PA)) catalysts with various catalyst loadings. Fourier characterized them trans-forms infrared spectroscopy, nuclear magnetic resonance spectrum, gel permeation chromatography and thermogravimetry analysis.

Results:
These macromolecular catalysts were used to catalyze acetone and benzaldehydes' direct asymmetric aldol reaction. The influence of the catalyst structure on the catalytic performance was studied, and the reaction conditions were optimized.

Conclusion:
The results showed that P(AA-co-PA) with 50 mol% catalyst loading had excellent cata-lytic performance, much higher than that of L-proline and L-hydroxyproline. Its recovery was achieved by simple filtration. After being reused 7 times, its catalytic performance was still higher than that of L-proline.
Vitamin D Deficiency in Advanced Laryngeal Cancer and its Association
with Pharyngocutaneous Fistula Following Total Laryngectomy
Authors: Iravani Kamyar , Khosravi Yaaghoob , Doostkam Aida , Soltaniesmaeili Amir 
Published: March 2023

Background:
Nutritional factors in developing some malignancies have been investigated
recently.

Objective:
In this study, we evaluated the role of vitamin D in advanced laryngeal cancer and its association
with the development of pharyngocutaneous fistula (PCF) following total laryngectomy.

Study Design:
A cross-sectional, case-control study was conducted.

Methods:
Fifty-five patients with advanced laryngeal cancer referred for total laryngectomy were
included. We also considered 55 healthy individuals after matching age and sex as a control group.
Serum levels of 25(OH)D3 were measured using a commercially available enzyme-linked immunosorbent
assay (ELISA) kit. The association of serum 25(OH)D3 with PCF following total
laryngectomy was also determined.

Results:
Vitamin D was significantly lower in patients with advanced laryngeal cancer than in the
control group (p < 0.001). Moreover, our results showed that a mean serum concentration of
25(OH)D3 in patients with PCF was significantly lower than in patients without PCF (p < 0.001).

Conclusion:
Vitamin D deficiency is highly prevalent in advanced laryngeal cancer, most pronounced
in those who develop a PFC following total laryngectomy.
Electrospun Propolis-coated PLGA Scaffold Enhances the Osteoinduction
of Mesenchymal Stem Cells
Authors: Askari Mohammad , Jadid Tavaf Maryam , Ghorbani Masoud , Yazdanian Mohsen , Moghaddam Mehrdad Moosazadeh 
Published: March 2023

Background:
Major injuries that are caused by trauma and cancer can not be repaired through
bone remodeling. The goal of bone regeneration by tissue engineering approaches is to fabricate bone
implants in order to restore bone structure and functions. The use of stem cells and polymer scaffolds
provides the conditions for tissue regeneration based on tissue engineering.

Objective:
This study aimed to fabricate a combined matrix of poly(lactide-co-glycolide) (PLGA) and
propolis extract, which is a mixture of pollen and beeswax collected by bees from certain plants and has
long been used in traditional herbal medicine, to promote the osteogenic differentiation of human adipose-
derived mesenchymal stem cells (AD-MSCs).

Methods:
The scaffold was fabricated through electrospinning and was immersed in a propolis extract
solution. Then, AD-MSCs were cultured and differentiated into the osteogenic lineage. The cell viability
on the scaffold was evaluated by MTT assay. Osteogenic differentiation of the seeded stem cells was
detected by evaluating calcium content, alkaline phosphatase (ALP) activity, and the expression of bonespecific
genes.

Results:
The viability of cells was not affected by propolis-coated and uncoated fabricated scaffolds,
while higher calcium content, ALP activity, and expression of RUNX-2, type I collagen, osteocalcin, and
osteonectin were observed in cells differentiated on propolis-coated PLGA scaffold on days 7, 14, and 21
of differentiation compared to PLGA scaffold.

Conclusion:
The results of this study showed that the presence of propolis in the scaffold could lead to
better cell attachment and strengthen the osteoinduction process in stem cells.
Piezo1-ERK1/2-YAP Signaling Cascade Regulates the Proliferation of
Urine-derived Stem Cells on Collagen Gels
Authors: Wang Xiaoya , Li Ling , Sun Bishao , Hou Xianglin , Song Siqi , Shi Chunying , Chen Wei 
Published: March 2023

Background:
Urine-derived stem cells (USCs) were considered to be an ideal source of stem
cells for repairing urological diseases. However, the proliferative ability of USCs significantly decreased
when cultured on plastic dishes, which limited their clinical application. It was found that collagen gels
could promote the proliferation of USCs, but the underlying molecular mechanisms were unclear.

Objective:
The study aims to investigate the role of the mechanically activated cation channel Piezo1 and
the transcriptional coactivator YAP in the regulation of proliferation of USCs on collagen gels.

Methods:
USCs were cultured on collagen gels (group COL), or plastic dishes (group NON). MTT assay,
Scratch assay, EDU staining, and immunofluorescence (IF) of Ki67 were performed to evaluate the
proliferation of USCs; IF of YAP was conducted to observe its nuclear localization; calcium imaging
experiment was executed to evaluate the function of Piezo1; western blot was used to compare changes in
protein expression of YAP, LATS1, ERK1/2, and p-ERK1/2. In addition, the regulatory effect of YAP on
the proliferative capacity of USCs was confirmed by intervening YAP with its inhibitor verteporfin (VP);
and the inhibitor or activator of Piezo1, GsMTx4 or Yoda1 was used to explore the effect of Piezo1 on the
nuclear localization of YAP, the proliferation of USCs and the regeneration of injured bladder.

Results:
The results showed that cell proliferation was significantly enhanced in USCs in the COL group
with the nuclear accumulation of YAP compared with the NON group and VP attenuated these effects.
The expression and function of Piezo1 were higher in the COL group compared with the NON group.
Blockage of Piezo1 by GsMTx4 decreased nuclear localization of YAP, the proliferation of USCs, and
caused the failure of bladder reconstruction. Activation of Piezo1 by Yoda1 increased the nuclear
expression of YAP, and the proliferation of USCs, which further improved the regeneration of the injured
bladder. Finally, the ERK1/2 rather than LATS1 was revealed to participate in the Piezo1/YAP signal
cascades of USCs proliferation.

Conclusion:
Taken together, Piezo1-ERK1/2-YAP signal cascades were involved in regulating the
proliferation ability of USCs in collagen gels which would be beneficial for the regeneration of the bladder.
The Potential Role of Vitamin D Supplementation in Cognitive
Impairment Prevention
Authors: Zeqaj Iris , Piffero Roberto , Calzaducca Elisa , Pirisi Mario , Bellan Mattia 
Published: March 2023

Background:
Vitamin D is implicated in many processes in the central nervous system
(CNS), such as neurogenesis, neurotransmitter synthesis, synaptogenesis and protection against oxidative
stress, thereby exerting a neuroprotective effect.

Objective:
In the present review, we aimed to evaluate the potential benefit(s) of vitamin D supplementation
for CNS aging in different clinical contexts.

Methods:
We performed a literature search, looking for clinical trials and randomized clinical trials evaluating
the effect of vitamin D supplementation on different endpoints related to cognitive outcomes.

Results:
Firstly, we identified 16 papers dealing with the impact of vitamin D supplementation on
cognitive function in healthy subjects; the current literature suggests a real role for vitamin D supplementation
in the prevention of cognitive decay in this clinical setting. Conversely, two papers suggest
that vitamin D supplementation may be beneficial in patients with mild cognitive impairment (MCI).
Finally, current data on vitamin D in Alzheimer’s disease are contradictory.

Conclusion:
Vitamin D supplementation may improve the cognitive outcomes of patients with MCI,
whereas there is no evidence that it may prevent dementia or modulate the course of Alzheimer’s disease.
Molecular Docking, Multicomponent One-pot Synthesis of Pyrimidine
Derivatives as Anti-mycobacterial Agents
Authors: Chandra  Panda Krishna , Kumar B.V.V. Ravi , Sahoo Biswa Mohan , Chandrasekaran Balakumar , Swain Parijat 
Published: March 2023

Background:
Molecular docking study is used significantly in the drug discovery process for predicting the interaction between drug and receptor. This technique has been used commonly to identify the binding affinity and orientation of drug molecules at the binding site of the target. The main objectives of docking studies include accurate modeling of molecular structure and precise prediction of the biological activity of the drug molecules.

Methods:
Based on this concept, a series of 2-amino-6-(substituted phenyl)-4-oxo-4,5-dihydropyrimidine-5-carbonitrile derivatives have been designed and synthesized via multi-component reaction. The synthetic protocol involves the one-pot, three-component reaction be-tween equimolar quantities of substituted benzaldehydes, ethyl cyanoacetate, and guanidine in an ethanolic sodium hydroxide solution.

Results:
The characterization of the titled compounds was carried out by assessing infrared spectroscopy (IR), proton nuclear magnetic resonance (1H-NMR), and mass spectrometry (MS) data. The synthesized compounds were screened for their in vitro anti-mycobacterial activity by using the luciferase reporter phage (LRP) assay method.

Conclusion:
The determination of anti-mycobacterial activity was carried out in terms of the percent reduction in the relative light unit (RLU). The test compounds displayed significant ac-tivity against Mycobacterium strain H37Rv in comparison to isoniazid as a standard drug.
Biological Activities of Papaya (Carica papaya L.) Seed, Rind and Pulp Oil
Extracts
Authors: Getahun Aynalem , Yusuf Zekeria , Desta Mulugeta 
Published: March 2023

Background:
Carica Papaya L. is widely cultivated for food and traditional medicine throughout the World due to its rich source of bioactive compounds in its fruit, leaves, latex and stem.

Objective:
The aim of this work was to assess the physicochemical properties, and antioxidant and antimicrobial activities of oils extracted from papaya seeds, fruit pulp and rind using the solvent ex-traction method.

Methods:
Hexane mediated extraction of oil was made. Then, physicochemical properties of the oil extracts were based on the determination of oil yield, specific gravity, acid value, free fatty acid and peroxide value, while the antioxidant activities were conducted based on ascorbic acid content, DPPH and hydrogen peroxide free radical scavenging activities. The antimicrobial experiment was conducted based on disc diffusion and broth dilution methods.

Results:
The result of physicochemical properties of papaya seeds, pulp and rind oil extracts demon-strated significantly the highest oil yield (42.50%) and specific gravity (0.85) obtained for seed oil. Significantly the highest DPPH (19.35%), and hydrogen peroxide scavenging activities (32.45%) were recorded for seed oil extract. The strongest antibacterial activity with maximum zone of inhibition (18.50 mm), minimum inhibitory concentration MIC (0.03 μg/mL) and minimum bactericidal concen-tration MBC (0.05 μg/mL) were recorded for seed oil extract against S. pyogenes. However, the strong-est antifungal activity with maximum zone of inhibition (16.75 mm), MIC (0.05 μg/mL) and MFC (0.10 μg/mL) against C. albicans was recorded for rind oil extract against C. albicans.

Conclusion:
It can be concluded from the result of this study that seed oil was found to have antioxidant and antibacterial potential but fruit rind oil extract was the most effective antifungal in papaya.
Research on Wheel Out-of-round Fault Diagnosis Based on Vibration Data
Images
Authors: Sun Peng , Yao Huiming , Yuan Chunping 
Published: March 2023

Background:
The wheel out-of-round fault of urban rail vehicles has a very important impact
on the safe operation of urban rail trains. Therefore, it is of great significance to achieve an accurate
diagnosis of the wheel out-of-round fault of trains.

Objective:
The purpose of this paper is to summarize the diagnosis methods of the wheel out-of-round
fault, and propose a new diagnosis method based on vibration data images, which can effectively identify
the wheel out-of-round fault.

Methods:
The one-dimensional vibration signal is converted into a two-dimensional texture matrix.
The Statistical Geometrical Features (SGF) method extracts the feature information of the twodimensional
gray image and combines it with a support vector machine for training and recognition to
achieve the fault diagnosis of the wheel out-of-roundness.

Results:
The feasibility and accuracy of the method are verified by simulation and experimental signal
analysis, respectively. The experimental results show that the overall recognition accuracy of the model
simulation data and the two-wheel experimental bench data exceeds 91%, exhibiting significantly
high fault identification accuracy.

Conclusion:
In this paper, a wheel out-of-round fault diagnosis model based on vibration data images
has been established by analyzing the vertical dynamic signal of the axle box, which has the advantages
of fast recognition in combination with two-dimensional grey-scale images, no signal preprocessing,
and high recognition accuracy. It provides a new method for monitoring and diagnosing
wheel out-of-round faults in urban rail vehicles.
COVID-19 Study, Diagnostic and Therapeutic Transition
Authors: Lu Da-Yong , Lu Ting-Ren 
Published: March 2023

Introduction:
The outbreak of coronavirus (severe acute respiratory syndrome
coronavirus2, COVID-19, SARS-CoV-2) in Wuhan, China occurred three years ago. However,
the healthcare state and legislature for COVID-19 varied greatly worldwide. After three
years, the social life of most countries worldwide is gradually back to normal. Diagnosis and
therapeutics worldwide are formalized now. Improvement of the knowledge about this devastating
disease will shed new light on its management and spawn the development of new
counter measures. Due to the differences in socioeconomic conditions and policies worldwide,
the diagnostic and therapeutic transition should be established. The schedules and techniques
of vaccines, drugs, or other therapeutic strategies could be formalized in the future.
The origin and hidden nature of COVID-19 biology (relationship between viral strain and
drug targeting) should be further investigated. Knowledge and opinion breakthroughs may
significantly heighten the quality of preventive and therapeutic strategies against COVID-19.
To further stabilize the global situation, the issues of viral spread and induced mortality
should be emphasized. Existing animal models, pathophysiological knowledge, and therapeutics
for different infected patients played vital roles. The diagnostic widening, variants of
COVID, and therapeutic selection worldwide totally solve the complex outcomes and promote
the curability for infected patients. Different diagnostic platforms can reach different
therapeutic selections, responses, and benefits in the clinic. It will provide advanced diagnostic
dimensions, therapeutic paradigms, and drug selection strategies for the purpose of the
greatest benefiting and recoveries of COVID-19 patients. To speed up the global fight against
COVID-19, biomedical knowledge, prophylactic vaccines, and therapeutic paradigms should
be updated in dynamic states.
Incorporating Consumers’ Needs in Nutrition Apps to Promote and Maintain Use: Mixed Methods Study
Authors: van der Haar Sandra , Raaijmakers Ireen , Verain Muriel C D , Meijboom Saskia 
Published: March 2023

Background
Nutrition apps seem to be promising tools for supporting consumers toward healthier eating habits. There is a wide variety of nutrition apps available; however, users often discontinue app use at an early stage before a permanent change in dietary behavior can be achieved.

Objective
The main objective of this study was to identify, from both a user and nonuser perspective, which functionalities should be included in nutrition apps to increase intentions to start and maintain use of these apps. A secondary objective was to gain insight into reasons to quit using nutrition apps at an early stage.

Methods
This study used a mixed methods approach and included a qualitative and a quantitative study. The qualitative study (n=40) consisted of a home-use test with 6 commercially available nutrition apps, followed by 6 focus group discussions (FGDs) to investigate user experiences. The quantitative study was a large-scale survey (n=1420), which was performed in a representative sample of the Dutch population to quantify the FGDs’ results. In the survey, several app functionalities were rated on 7-point Likert scales ranging from 1 (very unimportant) to 7 (very important).

Results
A total of 3 different phases of app use, subdivided into 10 user-centric app aspects and 46 associated app functionalities, were identified as relevant nutrition app elements in the FGDs. Relevance was confirmed in the survey, as all user-centric aspects and almost all app functionalities were rated as important to include in a nutrition app. In the starting phase, a clear introduction (mean 5.45, SD 1.32), purpose (mean 5.40, SD 1.40), and flexible food tracking options (mean 5.33, SD 1.45) were the most important functionalities. In the use phase, a complete and reliable food product database (mean 5.58, SD 1.41), easy navigation (mean 5.56, SD 1.36), and limited advertisements (mean 5.53, SD 1.51) were the most important functionalities. In the end phase, the possibility of setting realistic goals (mean 5.23, SD 1.44), new personal goals (mean 5.13, SD 1.45), and continuously offering new information (mean 4.88, SD 1.44) were the most important functionalities. No large differences between users, former users, and nonusers were found. The main reason for quitting a nutrition app in the survey was the high time investment (14/38, 37%). This was also identified as a barrier in the FGDs.

Conclusions
Nutrition apps should be supportive in all 3 phases of use (start, use, and end) to increase consumers’ intentions to start and maintain the use of these apps and achieve a change in dietary behavior. Each phase includes several key app functionalities that require specific attention from app developers. High time investment is an important reason to quit nutrition app use at an early stage.
Patients’ Experiences Using a Mobile Health App for Self-Care of Heart Failure in a Real-World Setting: Qualitative Analysis
Authors: Madujibeya Ifeanyi , Lennie Terry A , Pelzel Jamie , Moser Debra K 
Published: March 2023

Background
Publicly available patient-focused mobile health (mHealth) apps are being increasingly integrated into routine heart failure (HF)–related self-care. However, there is a dearth of research on patients’ experiences using mHealth apps for self-care in real-world settings.

Objective
The purpose of this study was to explore patients’ experiences using a commercially available mHealth app, OnTrack to Health, for HF self-care in a real-world setting.

Methods
Patient satisfaction, measured with a 5-point Likert scale, and an open-ended survey were used to gather data from 23 patients with HF who were provided the OnTrack to Health app as a part of routine HF management. A content analysis of patients’ responses was conducted with the qualitative software Atlas.ti (version 8; ATLAS.ti Scientific Software Development GmbH).

Results
Patients (median age 64, IQR 57-71 years; 17/23, 74% male) used OnTrack to Health for a median 164 (IQR 51-640) days before the survey. All patients reported excellent experiences related to app use and would recommend the app to other patients with HF. Five themes emerged from the responses to the open-ended questions: (1) features that enhanced self-care of HF (medication tracker, graphic performance feedback and automated alerts, secured messaging features, and HF self-care education); (2) perceived benefits (provided assurance of safety, improved HF self-care, and decreased hospitalization rates); (3) challenges with using apps for self-care (giving up previous self-care strategies); (4) facilitators (perceived ease of use and availability of technical support); and (5) suggested improvements (streamlining data entry, integration of apps with an electronic medical record, and personalization of app features).

Conclusions
Patients were satisfied with using OnTrack to Health for self-care. They perceived the features of the app as valuable tools for improving self-care ability and decreasing hospitalization rates. The development of apps in collaboration with end users is essential to ensure high-quality patient experiences related to app use for self-care.