bluuebunny/crossref_metadata_2025_split huggingface dataset:
Program temperature‐controlled drying: An effective way to improve the quality of hot‐air dried shiitake mushrooms
Authors: Liu Zhenbin , Luo Jia , Chitrakar Bimal , Liu Wenchao , Wang Dong , E Hengchao , Sun Zhenying , Li Hongbo , Wei Xinyu , Hu Liangbin , Zhang Jiayi , Mo Haizhen , Weng Rui 
Published: January 2025

AbstractThis study applied program temperature‐controlled drying (PTCD) to optimize the hot‐air drying process for shiitake mushrooms, adjusting the drying temperature based on activity changes of γ‐glutamyl transpeptidase (γ‐GTase) and cysteine sulfoxide lyase (C‐S lyase). Compared with constant temperature drying, PTCD (ST_75 and ST_150) significantly enhanced the umami and aroma profiles and sulfur compounds, increasing the levels of key flavor compounds such as glutamic acid and 5′‐GMP. Moreover, PTCD improved rehydration capacity (515.17%) and reduced shrinkage (12.43%) for ST_150 samples, achieving superior texture and color retention. Nutritional analysis indicated that PTCD better preserved nutrients such as ergothioneine, ergosterol, and purines, with ergosterol content reaching 9953.22 µg/g in the ST_150 group. This study provides theoretical support for improving the quality of hot‐air‐dried shiitake mushrooms in industrial applications.Practical ApplicationAlthough hot air drying is widely used for mushrooms, it is typically conducted using constant temperature methods. The application of PTCD and its effects on mushroom product quality have been scarcely studied. This study proved that compared with constant drying temperature, PTCD could significantly improve the flavor, texture, and nutrition retention of dried products. This may provide scientific foundation for the industrial application of hot air drying of shiitake mushrooms with improved quality.
An innovative approach for lipid‐rich food preservation: peanut skin microcapsules
Authors: Bergesse Antonella Estefanía , Camiletti Ornella Francina , Prieto María Cecilia , Ryan Liliana Cecilia , Grosso Nelson Rubén , Nepote Valeria 
Published: January 2025

AbstractThis study evaluates the microencapsulation of peanut skin phenolic compounds by spray drying, assessing their physicochemical properties and storage stability and the protective effect against oxidative deterioration in walnut kernels. Extraction yield, total phenolic content, and HPLC‐ESI‐MS/MS analysis were performed on peanut skin crude extract (PCE). Microencapsulation of PCE with 10%, 20%, and 30% maltodextrin via spray drying was conducted. The drying yield, phenolic encapsulation efficiency, moisture content, morphology, particle size, and stability during dry storage (23°C) and in water (23 and 100°C) were assessed for the microcapsules. PCE contained 950.29 mg GAE/g of total polyphenolic compounds, primarily hydroxycinnamic acid‐derived phenolic acids and procyanidins. Microcapsules with 20% maltodextrin exhibited the best properties (drying yield, encapsulation efficiency, and stability). These microcapsules were incorporated into an edible chickpea‐based coating, which was applied to walnuts and stored at 40°C for 15 days. Peroxide value, conjugated dienes, volatile compounds, and fatty acid profile were analyzed on samples from storage. The chickpea‐based coating combined with PCE microcapsules effectively preserves walnut quality during storage, offering a viable and natural alternative to synthetic antioxidants such as BHT, addressing current concerns in food preservation.Practical ApplicationPeanut skins are a byproduct of the peanut industry with low commercial value. These skins are rich in polyphenols, which exhibit potent antioxidant activity. This study investigates the microencapsulation of polyphenolic peanut extract and its incorporation into a chickpea‐based edible coating. The prepared coating demonstrated a remarkable protective effect against lipid oxidation in walnuts, extending their shelf life. These findings present a sustainable strategy that adds value to agro‐industrial residues and aligns with circular economy principles. This innovation offers a natural and effective solution to enhance the stability and quality of lipid‐rich foods.
Effects of probiotic coating and vacuum packaging on the microbial safety and quality of fresh‐cut cantaloupe stored at different temperatures
Authors: Hua Qian , Li Dan 
Published: January 2025

AbstractFresh‐cut cantaloupes are highly susceptible to contamination by foodborne pathogens and spoilage‐causing microorganisms. This study evaluated the efficacy of a probiotic coating produced by fermenting Lactiplantibacillus plantarum 299 V in pomelo peel extract in combination with vacuum packaging in controlling the microbial loads of fresh‐cut cantaloupe during storage. As temperature abuse is common in transportation and at retail sale of such products in many countries, we evaluated their efficacy at different temperatures. Both the probiotic edible coating and vacuum packaging, when used alone, effectively inhibited microbial growth in fresh‐cut cantaloupe at all the tested temperatures. The combination had a statistically significant synergistic effect (p < 0.05) on reducing psychrotrophic total counts (4.5 ± 0.3 log lower than control on day 9) and yeast and mold (>3.1 log lower than control on day 9) at 4°C, Listeria monocytogenes (2.9 ± 0.2 log lower than control on day 6) at 12°C, and yeast and mold (3.1 ± 0.7 log lower than control on day 2) and L. monocytogenes (4.6 ± 0.4 log lower than control on day 2) at room temperature (23 ± 2°C). The solution proposed in this study has the potential to increase the resilience of products to temperature abuse, which can impact the safety of the food supply.
Enhancing beer authentication, quality, and control assessment using non‐invasive spectroscopy through bottle and machine learning modeling
Authors: Harris Natalie , Gonzalez Viejo Claudia , Zhang Jiaying , Pang Alexis , Hernandez‐Brenes Carmen , Fuentes Sigfredo 
Published: January 2025

AbstractFraud in alcoholic beverages through counterfeiting and adulteration is rising, significantly impacting companies economically. This study aimed to develop a method using near‐infrared (NIR) spectroscopy (1596–2396 nm) through the bottle, along with machine learning (ML) modeling for beer authentication, quality traits, and control assessment. For this study, 25 commercial beers from different brands, styles, and three types of fermentation were used. To obtain the ground‐truth data, a quantitative descriptive analysis was conducted with 11 trained panelists to evaluate the intensity of 16 sensory descriptors, and volatile aromatic compounds were analyzed using gas chromatography–mass spectroscopy (GC–MS). The ML models were developed using artificial neural networks with NIR absorbance values as inputs to predict (i) type of fermentation (Model 1), (ii) intensity of 16 sensory descriptors (Model 2), and (iii) peak area of volatile aromatic compounds (Model 3). All models resulted in high overall accuracy (Model 1: 99%; Model 2: R = 0.92; Model 3: R = 0.94), and model deployment for new beer samples showed high performance (Model 1: 95%; Model 2: R = 0.83). This method enables brewers and retailers to analyze beers without opening bottles, preventing quality assurance issues, fraud, and provenance concerns. Further model training with new targets could assess additional quality traits like physicochemical parameters and origin.Practical ApplicationNear‐infrared spectroscopy coupled with ML modeling is a novel method for assessing beer quality and authentication through the bottle. It serves as a rapid, accurate tool for predicting sensory and aroma profiles without opening the bottle. Additionally, it monitors quality traits during transport and storage.
Comparative analysis of the structure and content of N‐glycans from different commercial whey protein materials
Authors: Zhang Wanyi , Peng Qiuqi , Yuan Linhan , Wu Caiwen , Wang Mengqi , Li Hongbo , Li Hongjuan , Yu Jinghua 
Published: January 2025

AbstractInfant formulas are constantly being updated and upgraded, and N‐glycans are functional glycans that have not been fully exploited to date. Commercial whey protein materials are often used as basic ingredients in infant formulas. Therefore, it is important to study N‐glycans in commercial whey protein materials. We used matrix‐assisted laser desorption/ionization time of flight mass spectrometry (MALDI‐TOF‐MS) and ion chromatography to analyze N‐glycans in bovine lactoferrin (Lf), whey protein isolate (WPI), whey protein concentrate 70 (WPC 70), goat whey protein powder 50, demineralized whey powder 90 (D90), and desalted goat whey powder. The results showed that 30, 6, 28, 16, 8, and 9 N‐glycans were found in Lf, D90, desalted goat whey powder, WPI, WPC 70, and goat whey protein powder 50, respectively. A total of four structures of N‐glycans were detected in this study. Only bovine Lf and WPC 70 contained fucosylated and sialylated binding (SFN‐type) glycan structures. Regarding content, WPC 70 showed the highest yield of 14.5 mg/g, and the degree of sialylation was higher than fucosylation. This study provides a potential basis for the future use of commercial whey protein materials in dairy products such as infant formula.
Exploring Environmental Microfungal Diversity Through Serial Single Cell Screening
Authors: Mariz Joana , Nawaz Ali , Bösch Yvonne , Wurzbacher Christian 
Published: January 2025

ABSTRACTKnown for its remarkable diversity and ecological importance, the fungal kingdom remains largely unexplored. In fact, the number of unknown and undescribed fungi is predicted to exceed the number of known fungal species by far. Despite efforts to uncover these dark fungal taxa, we still face inherent sampling biases and methodological limitations. Here, we present a framework that combines taxonomic knowledge, molecular biology and data processing to explore the fungal biodiversity of enigmatic aquatic fungal lineages. Our work is based on serial screening of environmental fungal cells to approach unknown fungal taxa. Microscopic documentation is followed by DNA analysis of laser micro‐dissected cells, coupled with a ribosomal operon barcoding step realised by long‐read sequencing, followed by an optional whole genome sequencing step. We tested this approach on a range of aquatic fungal cells mostly belonging to the ecological group of aquatic hyphomycetes derived from environmental samples. From this initial screening, we were able to identify 60 potentially new fungal taxa in the target dataset. By extending this methodology to other fungal lineages associated with different habitats, we expect to increasingly characterise the molecular barcodes of dark fungal taxa in diverse environmental samples. This work offers a promising solution to the challenges posed by unknown and unculturable fungi and holds the potential to be applied to the diverse lineages of undescribed microeukaryotes.
Assessing Metabolic Ageing via DNA Methylation Surrogate Markers: A Multicohort Study in Britain, Ireland and the USA
Authors: Xu Kexin , Hernández Belinda , Arpawong Thalida Em , Camuzeaux Stephane , Chekmeneva Elena , Crimmins Eileen M. , Elliott Paul , Fiorito Giovani , Jiménez Beatriz , Kenny Rose Anne , McCrory Cathal , McLoughlin Sinead , Pinto Rui , Sands Caroline , Vineis Paolo , Lau Chung‐Ho E. , Robinson Oliver 
Published: January 2025

ABSTRACTMetabolomics and epigenomics have been used to develop ‘ageing clocks’ that assess biological age and identify ‘accelerated ageing’. While metabolites are subject to short‐term variation, DNA methylation (DNAm) may capture longer‐term metabolic changes. We aimed to develop a hybrid DNAm‐metabolic clock using DNAm as metabolite surrogates (‘DNAm‐metabolites’) for age prediction. Within the UK Airwave cohort (n = 820), we developed DNAm metabolites by regressing 594 metabolites on DNAm and selected 177 DNAm metabolites and 193 metabolites to construct ‘DNAm‐metabolic’ and ‘metabolic’ clocks. We evaluated clocks in their age prediction and association with noncommunicable disease risk factors. We additionally validated the DNAm‐metabolic clock for the prediction of age and health outcomes in The Irish Longitudinal Study of Ageing (TILDA, n = 488) and the Health and Retirement Study (HRS, n = 4018). Around 70% of DNAm metabolites showed significant metabolite correlations (Pearson's r: > 0.30, p < 10−4) in the Airwave test set and overall stronger age associations than metabolites. The DNAm‐metabolic clock was enriched for metabolic traits and was associated (p < 0.05) with male sex, heavy drinking, anxiety, depression and trauma. In TILDA and HRS, the DNAm‐metabolic clock predicted age (r = 0.73 and 0.69), disability and gait speed (p < 0.05). In HRS, it additionally predicted time to death, diabetes, cardiovascular disease, frailty and grip strength. DNAm metabolite surrogates may facilitate metabolic studies using only DNAm data. Clocks built from DNAm metabolites provided a novel approach to assess metabolic ageing, potentially enabling early detection of metabolic‐related diseases for personalised medicine.
Long‐term effect of Roux‐en‐Y gastric bypass versus sleeve gastrectomy on reflux and Barrett's oesophagus: a randomized controlled trial
Authors: Lee Young Min , Barazanchi Ahmed , Robertson Jason , Murphy Rinki , Booth Michael W. C. 
Published: January 2025

AbstractBackgroundLaparoscopic sleeve gastrectomy (LSG) is a potentially refluxogenic operation while Laparoscopic Roux‐en‐Y Gastric Bypass (LRYGB) is regarded as an anti‐reflux procedure. The aim of this study is to compare long‐term incidence of Barrett's Oesophagus (BO) and gastroesophageal reflux disease (GORD) following LSG and LRYGB.MethodsParticipants of a double‐blinded randomized controlled trial comparing banded LRYGB and LSG for remission of type 2 diabetes were contacted to take part. A gastroscopy was performed. Primary outcome was endoscopic and histologic evidence of BO. Secondary outcomes included reflux and regurgitation scores, presence of oesophagitis, proton‐pump inhibitor (PPI) usage, Body Mass Index (BMI), and percentage excess weight loss (%EWL).ResultsForty‐eight of 109 patients were enrolled into the study (LSG 26 vs. LRYGB 22). Mean follow‐up was 7.5 years for the LSG group, and 7.4 years for the RYGB group (P = 0.22). 8 LSG patients had BO while 3 LRYGB patients had BO (30.8%vs13.6%, P = 0.19). There was no significant difference in the mean reflux (8.1 ± 9.4(0–36) vs. 9.3 ± 8.8(0–34), P = 0.47) and regurgitation scores (7.7 ± 6.9(0–22) vs. 11.5 ± 10.5(0–44), P = 0.23) for LSG versus LRYGB patients or between those with and without BO. PPI usage before and after surgery was 6/26 (23.1%) versus 13/26 (50.0%) and 8/22 (36.4%) versus 12/22 (54.5%) for LSG and LRYGB patients respectively. PPI usage in patients with and without BO was 7/11 versus 18/37. EWL was significantly greater (P = 0.0013) in the LRYGB group (74.8 ± 28.1%) compared to LSG group (49.7 ± 18.7%).ConclusionsLong‐term incidence of BO trended towards but was not significantly higher for LSG compared to LRYGB group. We support routine endoscopic surveillance for bariatric patients.
Methods for early detection of selective loss of visual function in diseases of the retina
Authors: Barbur John L. 
Published: January 2025

Retinal mechanisms evolved to extract and code efficiently the most important information present in retinal images. Luminance contrast, ‘red/green’, ‘yellow/blue’, ‘white‐black’, and ‘light‐flux’ channels extract the signals needed to account for most attributes of our visual perception, including the ability to judge the level of ambient lighting. By employing two types of photoreceptors, rods and cones, the visual system achieves an extraordinarily large, dynamic range of useful vision, but this involves extensive compromises. The spatial, temporal and chromatic properties of the signals extracted from the retinal image very greatly with the level of ambient illumination. Visual acuity, contrast sensitivity, red/green and yellow/blue colour vision and motion and rapid flicker sensitivity, all worsen with decreasing light level. The extent to which visual performance is compromised in the mesopic range is also affected by normal aging and retinal disease.In this lecture I shall describe a number of advanced vision and optometric tests designed to reduce within subject variability, to account for normal age‐related changes, to isolate selectively red/green and yellow/blue colour mechanisms and most importantly, to test specifically for the rate of loss of visual sensitivity in the mesopic range. Results from extensive investigations in patients with diabetes, age‐related macular degeneration and glaucoma will be used to illustrate the usefulness of these tests in the early detection of retinal disease. Unique results from single patient studies will also be presented to illustrate some visual consequences of abnormal interaction of rod and cone signals in the mesopic range.
Hyperbaric oxygen therapy for branch retinal artery occlusion: A case series
Authors: Zarzecki Mateusz , Zawadzka Izabela , Mitera Blanka , Wojewodzka‐Zelezniakowicz Marzena , Konopińska Joanna 
Published: January 2025

Aims/Purpose: To evaluate the effectiveness of Hyperbaric Oxygen Therapy (HBOT) in improving visual acuity in patients with BRAO.Methods: The Case Series analysis of 3 patients affected by BRAO.Results: In the case series we described, patients underwent HBOT at 5, 36 and 48 hours after the onset of ocular symptoms. In all presented cases visual acuity improved quantitatively and qualitatively.Conclusions: Our case series confirms beneficial effect of HBOT on final VA in patients after an episode of BRAO. HBOT might be a rescue treatment at an early stage of BRAO, particularly to the time of a conceivable reperfusion. The further research on broader groups of patients and randomized controlled trials are needed to confirm this assumption.References: Retinal artery occlusion (RAO) is one of the ophthalmic emergencies and may be the first harbinger of systemic cardiovascular disease. Central RAO (CRAO) may leads to serious visual loss, while branch retinal artery occlusion (BRAO) has more benign symptoms and often resolves with less severe visual disfunction. The pathomechanism of RAO resembles an ischemic stroke and involves sudden closure of the retinal artery, leading to ischemia of the inner layers of the eye then to retinal infarction and permanent damage to sensory cells, resulting in irreversible loss of vision.
More Complex Investigations Improved Diagnoses of Community‐Acquired Pneumonia in Children With Different Vaccination Histories
Authors: Tukbekova Bibigul , Zhanpeissova Aliya , Kysabekova Akbota , Sarmankulova Gulmira , Turlybekova Saule 
Published: January 2025

ABSTRACTAimCommunity‐acquired pneumonia (CAP) significantly contributes to high infant mortality in Kazakhstan and developing effective treatment methods is critical. The aim of this study was to explore the microbiological and immunological characteristics of CAP in vaccinated and unvaccinated paediatric patients.MethodsThe study was carried out in the Regional Children's Clinical Hospital and the research centre of Karaganda Medical University, Republic of Kazakhstan. It comprised 85 children aged 2 months to 3 years who had been diagnosed with CAP and had received inpatient treatment from 2017 to 2019. Of these, 45 had complete pneumococcal vaccinations and 40 had not. Their CAP diagnoses were confirmed by microscopic, bacteriological and radiological methods and vaccination status. General clinical examinations were conducted.ResultsThe study showed the diagnostic and prognostic value of the level of cytokines, depending on the severity of the children's CAP. The level of monocytic chemoattractant protein‐1 was 2.1 pg/mL in the vaccinated children with mild CAP and 3.4 pg/mL in those with impaired immunisation. Pneumococcal vaccination affected the aetiological structure of CAP in early childhood.ConclusionMore complex clinical and immunological diagnostic methods increased the efficiency of diagnosing CAP in children with different vaccination histories.
Respiratory Syncytial Virus Vaccination Is Associated With Increased Odds of Preterm Birth
Authors: Kuitunen Ilari , Haapanen Marjut 
Published: January 2025

ABSTRACTAimTo analyse whether respiratory syncytial virus (RSV) vaccination during pregnancy increases the odds of preterm birth.MethodsA rapid review and meta‐analysis was performed. The main outcome was the risk of preterm (gestational week less than 37) birth. A fixed‐effects model was used to analyse pooled odds ratios (OR) with 95% confidence intervals (CI). Evidence certainty was assessed according to GRADE.ResultsWe included six randomised controlled trials with 17 656 births and two observational studies with 3446 births. The odds for preterm birth were increased in the randomised studies (OR 1.17, CI 1.02–1.34). No evidence of a difference was seen in the observational studies (OR 0.93, CI 0.69–1.25). Combined, these showed increased odds for preterm birth (OR 1.13, CI 1.00–1.27). Evidence certainty was rated to be moderate. When restricted to market‐approved vaccine, the odds seemed to be increased in RCTs (OR 1.21, CI 0.98–1.49).ConclusionBased on the available evidence, RSV vaccination seems to be associated with increased odds for preterm birth. RSV vaccination needs continuous population‐level observational safety data monitoring on the perinatal outcomes during the early phases of vaccine rollouts to detect possible safety signals and further confirm the magnitude of the effect on preterm birth.
Retrospective Cohort Study: Scope for Improvement—Barriers to Post‐Polypectomy Surveillance in the Integrated Technologies for Improved Polyp Surveillance Cohort
Authors: Mathews Charlotte , Nauman Aleena , Johnstone Mark , Stoops Reiss , Tham Alexander , Parsons Emma C. , Robb Kathryn A. , Sloan William , Lynch Gerard , Edwards Joanne , McSorley Stephen T. ,   the INCISE collaborative group
Published: January 2025

ABSTRACTBackgroundAdherence to post‐polypectomy surveillance is poor despite evidence that it is associated with lower risk of future colorectal cancer.MethodsWe evaluated 6,210 bowel screening participants between 2009‐2016 in NHS Greater Glasgow and Clyde to assess potential barriers to post‐polypectomy surveillance.ResultsIncreasing deprivation (Scottish Index of Multiple Deprivation quintile 1 vs 5; OR 1.68; p < 0.001), and increasing comorbidity (Charlson Comorbidity Index 1‐2 vs 3‐4; OR 1.80; p < 0.001, vs ≥ 5; OR 3.31; p < 0.001), were associated with non‐surveillance in British Society of Gastroenterology 2002 intermediate/high‐risk patients, while ACE‐Inhibitor (OR 0.78; p < 0.001) and aspirin use (OR 0.34; p < 0.001) were associated with undergoing surveillance. The most deprived patients receiving surveillance had more metachronous polyps (54.0% vs 49.3%) and cancer (1.1% vs 0.4%) (p = 0.044).DiscussionPatients from more socioeconomically deprived areas are less likely to have appropriate post‐polypectomy surveillance, and are more likely to have metachronous polyps and colorectal cancer even when they do.ConclusionSurveillance strategies must take into account factors including socioeconomic deprivation and comorbidity exist to improve surveillance uptake in this group through the design of targeted interventions which move away from the current “one size fits all” approach.