bluuebunny/crossref_metadata_2025_split huggingface dataset:
Fabrication, characterization, and in vitro release of vitexin-loaded chitosan nanoparticles
Authors: Yoksan Rangrong , Noivoil Narumol 
Published: April 2023

Abstract
Vitexin is a natural bioactive compound with many functional properties. However, its poor water solubility and dispersion stability and short half-life limit its practical applications for targeting specific sites. The current work demonstrates that encapsulation of vitexin in chitosan nanoparticles via an oil-in-water emulsion followed by ionic gelation using pentasodium triphosphate overcomes these limitations. The successful loading of vitexin into chitosan nanoparticles was confirmed using ultraviolet-visible spectroscopy, Fourier-transform infrared spectroscopy, X-ray diffraction, thermogravimetric analysis, and differential scanning calorimetry. The vitexin-loaded chitosan nanoparticles were spherical, 50–250 nm in diameter, and had a negatively charged surface (zeta potential of -6.2 mV to -13.8 mV), 2.8–21.0% loading capacity (LC), and 16.6–58.2% encapsulation efficiency. Vitexin LC increased with increasing initial concentration. The vitexin-loaded chitosan nanoparticles showed antioxidant and lipid oxidation retardation activities that increased as a function of LC. Encapsulation of vitexin into chitosan nanoparticles could improve its dispersion stability in water. Vitexin release from chitosan nanoparticles into the buffer media was faster at pH 11 (completed within 10 days) than at pH 7 (80 days) and 3 (> 4 months). These results suggest that vitexin-loaded chitosan nanoparticles are stable in acidic and neutral pH media, and the loaded vitexin can potentially withstand the harsh gastrointestinal environment.
Benchmark of tools for in silico prediction of MHC class I and class II genotypes from NGS data
Authors: Claeys Arne , Merseburger Peter , Staut Jasper , Marchal Kathleen , Eynden Jimmy Van den 
Published: April 2023

Abstract
Background The Human Leukocyte Antigen (HLA) genes are a group of highly polymorphic genes that are located in the Major Histocompatibility Complex (MHC) region on chromosome 6. The HLA genotype affects the presentability of tumour antigens to the immune system. While knowledge of these genotypes is of utmost importance to study differences in immune responses between cancer patients, gold standard, PCR-derived genotypes are rarely available in large Next Generation Sequencing (NGS) datasets. Therefore, a variety of methods for in silico NGS-based HLA genotyping have been developed, bypassing the need to determine these genotypes with separate experiments. However, there is currently no consensus on the best performing tool.Results We evaluated 13 MHC class I and/or class II HLA callers that are currently available for free academic use and run on either Whole Exome Sequencing (WES) or RNA sequencing data. Computational resource requirements were highly variable between these tools. Three orthogonal approaches were used to evaluate the accuracy on several large publicly available datasets: a direct benchmark using PCR-derived gold standard HLA calls, a correlation analysis with population-based allele frequencies and an analysis of the concordance between the different tools. The highest MHC-I calling accuracies were found for Optitype (98.0%) and arcasHLA (99.4%) on WES and RNA sequencing data respectively, while for MHC-II HLA-HD was the most accurate tool for both data types (96.2% and 99.4% on WES and RNA data respectively).Conclusion The optimal strategy for HLA genotyping from NGS data depends on the availability of either WES or RNA data, the size of the dataset and the available computational resources. If sufficient resources are available, we recommend Optitype and HLA-HD for MHC-I and MHC-II genotype calling respectively.
New Fluorescence Derivatization Reagent for the Determination of 7 Unsaturated Fatty Acids in Oil Samples by Pre-Column Derivatization HPLC-FLD
Authors: Yu Shuiqiang , You Jinmao , Shi Xinxin , Zou Xiaocong , Lu Zhihao , Wang Yu , Song Cuihua 
Published: April 2023

Abstract
4-((2-Methyl-1,3-dioxo-2,3-dihydro-1H-benzo[de]isoquinoline-6-yl)amino)-Ethyl-4-Methyl Benzenesulfonate (MNDMB), was designed and synthesized, and it was first successfully applied to label unsaturated fatty acids and HPLC-FLD determination. MNDMB can quickly complete the derivatization reaction with unsaturated fatty acids within 40 min with K2CO3 as the catalyst. The derivatives exhibit high stability and strong fluorescence properties with excitation and emission wavelengths of 285 and 522 nm, respectively. The 7 unsaturated fatty acid derivatives were separated on an Agilent ZORBAX 300SB-C18 column with gradient elution, and good linear correlation coefficients were obtained for all unsaturated fatty acids with correlation coefficients of ≥ 0.9998. The method has low limits of detection (LODs) (4–21 nmol/L) and good precisions ( intraday relative standard deviations [RSDs] were 1.91–3.51%, and interday RSD were 3.33–5.41%). The method was used to analyze two edible oils and successfully detected some unsaturated fatty acids with recoveries ranging from 90.2 to 103.5%. The proposed method has several advantages including specific labeling, simple enrichment operation, and high sensitivity, demonstrating its great utility for monitoring trace unsaturated fatty acids in various complex matrices.
P16 promotes aging-induced lipid droplet accumulation via up-regulation of PCSK9 in hepatocytes
Authors: Hao Nannan , Peng Chengyi , Shi Yumeng , Liu Chang , Qiu Yulu , Yong Hui , Gu Xin , Wang Fang 
Published: April 2023

Abstract
Background: The accumulation of senescent cells promotes hepatic fat accumulation. P16, a proto-typical marker of senescent cells, is closely correlated to hepatic lipid accumulation. PCSK9 (proprotein convertase subtilisin/kexin type 9) plays a critical role in lipid metabolism via PCSK9/LDLR (low-density lipoprotein receptor) axis. This study aimed to explore the mechanism of p16 modulating PCSK9 expression to enhance hepatic lipid accumulation.
Methods: All aging mice (12 months old) were randomly assigned two groups: control group with HF (high fat) diet for 6 months, and medicine group with ABT263 (senolytic drug) treatment for 6 months in the presence of HF diet. To induce the senescent cells, cells were treated with bleomycin or adenovirus overexpressing p16 (ad-p16). Cells were treated with cell culture medium containing oleic acid (OA) and palmitic acid (PA) to mimic hepatic steatosis in vivo. The senescent cells were evaluated by SA-β-gal staining. For lipid droplets visualization, Oil red O and Nile red staining were performed. Eventually, the effect of p16 on PCSK9/LDLR axis was determined by Western blot and real-time quantitative polymerase chain reaction (RT-qPCR).
Results: We found ABT263 treatment markedly reduced lipid droplets, accompanied with dramatically decreased expression of p16 and PCSK9 in the liver. P16 silencing in senescent HL7702 inhibited lipid droplet accumulation, while p16 overexpression in AML12 remarkably increased lipid droplets, cellular content of total cholesterol and low-density lipoprotein cholesterol. Moreover, total PCSK9 protein level enhanced in p16-overexpressed hepatocytes, while LDLR significantly decreased in membrane and increased in cytoplasm in these cells. Mechanically, we found p16 overexpression inhibited K48-linked polyubiquitination of PCSK9.
Conclusions: These results indicate a novel role of p16 in lipid droplet accumulation through aberrant regulation of PCSK9/LDLR axis with PCSK9 ubiquitination in hepatocytes. Lowering p16 expression may be a novel strategy to reduce aberrant lipid metabolism in aging-related diseases.
Natural pterostilbene-grafted-chitosan for fabrication of ecological and durable antibacterial cotton fabric via lay-by-lay assembled coating
Authors: Wang Lili , Zhao Xiaomin , Li Hao , Wu Minghua 
Published: April 2023

Abstract
Natural antibacterial agents have tremendous potential for the fabrication of ecofriendly and durable antibacterial fabrics. Herein, natural pterostilbene (PL) was grafted onto chitosan (CS) by Mannich reaction to obtain PLCS with high antibacterial activity. Ecofriendly and durable antibacterial cotton fabric was fabricated via lay-by-lay assembled coating using PLCS as antibacterial agent. The results showed that under a molar ratio of CS and PL 1: 0.2 at 80 ℃, PLCS with grafting ratio of 17.3% attained the lowest minimum inhibitory concentration of 33.2 µg/mL and 16.6 µg/mL against S. aureus and E. coli, respectively. Compared with CS and PL, antibacterial activity of PLCS against S. aureus improved by 67.0% and 58.5%, and those against E. coli improved by 94.8% and 44.7%, respectively. Antibacterial rates of PLCS cotton fabric against S. aureus and E. coli reached up to 99.9% after 3 assembled layers and maintained more than 80% after 20 washing times. PLCS antibacterial fabrics possessed good wearing safety and wearing performances.
Exploring the Correlation between HAdV Infection and Respiratory Tract Infection Symptoms: A Study in Nanning, China
Authors: Qin Jianqiu , Yan Tengyue , Yin Liujiang , Yang Cheng , Wang Liang , Qiu Hong , Hu Yanling , Xu Bin 
Published: April 2023

Abstract
Background Human adenoviruses (HAdV) can cause various diseases, but there is limited information about the genotype diversity and epidemiology of HAdV associated with respiratory tract infections (RTIs) in Nanning.Methods From Jun 2019 to December 2021, throat swab, nasal swab, or nasopharyngeal swab samples were collected from hospitalized patients with RTIs, and Epidemiological data were analyzed using statistical software. HAdV were detected using a qPCR assay targeting a highly conserved 132-bp gene region of the HAdV hexon, and phylogenetic analysis was performed on the 875-bp hexon gene fragment.Results The age and sex distribution of HAdV-positive patients showed significant differences (P = 0.014 and P = 0.025, respectively). The majority of HAdV-positive patients were in the 0–6 age group (67.89%). The HAdV detection rate showed the highest prevalence in spring and autumn, peaking in February. In addition, a total of 37 HAdV-positive samples with 875-bp fragments were successfully genotyped and found to be circulating in Nanning from 2019 to 2021 with HAdV species B, C, and E.Conclusions HAdV prevalence is associated with age and season, with HAdV-B, HAdV-C, and HAdV-E co-circulating among hospitalized patients with RTIs in Nanning. The most frequent genotypes were HAdV-C1, HAdV-C6, and HAdV-E4.
Revisiting the cortisol reference ranges in humans: the role of demographics
Authors: Pai Jayeeta , Joshi Revan Kumar , Bhaskar Shanthi , Patil Seema , V. Subash B. , R Shriyanka , Iyengar Asha R. , Agarwal Nitin , Shorie Munish 
Published: April 2023

Abstract
The current study explores the effect of demographics on serum cortisol expression in a study group of 52 individuals to improve the current serum reference ranges to produce personalized expression profiles consequently increasing clinical confidence in the diagnosis. The serum cortisol concentration was inspected against demographical data like age, sex, and body mass index and showed an association with age and sex. The serum cortisol values also indicated a positive association with chronic illnesses however this finding requires a more focused study for establishment. Additionally, saliva samples are also collected from the same study group at the same time through drool and an absorbent sponge and correlated with serum values to draw an alternative route of serological testing. Salivary cortisol from drool showed a linear correlation of 0.51 and 0.52 with serum cortisol and with saliva samples collected using a porous sponge respectively. Overall, the study shows the role of demographics in shaping the reference ranges for cortisol, suggesting a path for developing personalized diagnostics. The study also highlights the efficacy of saliva as an alternative to serum cortisol to facilitate cortisol measurement for efficient stress management.
Prognostic value of post-induction medullary myeloid recovery by flow cytometry in acute myeloid leukemia
Authors: ROW Céline , Lechevalier Nicolas , VIAL Jean-Philippe , Mimoun Aguirre , Bastie Jean-Noël , caillot denis , Sleiman Emmanuel , Pigneux Arnaud , Leguay Thibault , Callanan Mary , Maynadie Marc , Béné Marie-Christine , Dumas Pierre-Yves , Guy Julien 
Published: April 2023

AbstractRisk stratification and treatment response evaluation are key features in acute myeloid leukemia (AML) management. Immunophenotypic and molecular approaches all rely on the detection of persisting leukemic cells by measurable residual disease techniques. A new approach is proposed here by assessing medullary myeloid maturation by flow cytometry through a myeloid progenitor ratio (MPR). The normal MPR range was defined using reference normal bone marrows (n= 48). MPR was considered balanced if between 1 and 4 and unbalanced if <1 or >4. MPR was retrospectively assessed at baseline and post-induction for 206 newly diagnosed AML patients eligible for intensive treatment from two different French centers. All AML baseline MPR were unbalanced and thus significantly different from normal MPR (p<0.0001). Patients with an unbalanced MPR after induction had worse 3-year overall survival (OS) (44.4%vs80.2%, HR, 2.96; 95%CI, 1.81-4.84, p<0.0001) and 3-year relapse free survival (RFS) (38.7%vs64.4%, HR, 2.11; 95%CI, 1.39-3.18, p<0.001). In multivariate analysis, post-induction unbalanced MPR was significantly associated with shorter OS and RFS regardless of the European LeukemiaNet 2010 risk stratification orNPM1/FLT3-ITD status. A balanced post-induction MPR conversely conferred favorable outcomes and reflects medullary myeloid recovery.
Existence and uniqueness for a nonlinear fractional integro-differential equation via fixed point theory and the maximum principal
Authors: Khaldi Soumia , Mecheraoui Rachid 
Published: April 2023

Abstract
In this paper, we discuss the existence and the uniqueness of positive solutions for a nonlinear fractional mixed Volterra-Fredholm integro-differential equation in the setting of HÖlder spaces via fixed point technique. Moreover, we establish some new results concerning the maximum principal to ensure the uniqueness of the solutions.
Naje Haje Toxins and Inflammatory Signaling Pathways Are Inhibited By Moringa Oleifera Leaf Fractions for Toxicity Testing Materials on Animal Embryos
Authors: Mafruchati Maslichah , Ismail Wan Iryani Wan 
Published: April 2023

Abstract

Blood toxicity, antioxidant poisoning, and nervous system poisoning are just a few of the unfavorable side effects of naja haje envenoming. Although Moringa oleifera has been used to treat a variety of toxicities brought on by snake venom, no studies have been conducted to determine its efficacy as an antivenom for Naja haje to investigate the effects of Moringa Oleifera leaf partitions on the inflammatory and antioxidant pathways that prevent N from passing through cells. There were 45 rats in total, broken up into 9 groups of 5 males each. Samples injections had a LD50 of 0 mg/kg in groups 2 through 9. While Group 2 was treated with saline solution, group 1 was given a placebo. Each participant in Group 3 received a shot of a very antigenic antivenom. Result showed that the acute anemia brought on by venom was significantly reduced by the partition of Moringa Oleifera ethanol extract and ethyl acetate. Alcohol is a powerful antivenom for haje venom. The antioxidant enzyme nuclear factor erythroid 2-related factor 2 (Nrf2), which is also present in serum and the brain, was more active before envenoming than it was after. Alternatives include extraction partitions, which increase Nrf2 while restarting regular antioxidant enzyme activity. The tissues of the treated envenomed rats also had lower concentrations of TNF- and interleukin-1.
Planting and farmers’ perception on environmental impacts of Moringa stenopetala Tree in Offa Woreda, Southern Ethiopia
Authors: Taye Tagay 
Published: April 2023

Abstract

Moringa stenopetala has increased a great consideration currently in Ethiopia due to its multiple uses. The study was accompanied in Offa district of Southern Ethiopia through the objective of evaluating planting and farmer’s perception of Moringa stenopetala tree on the environmental impacts. In order to attain these objectives, three potential sites were purposively selected due to the availability and distribution of M.stenopetala tree on the farmstead. Data collected from 116 respondents across wealth categories. The data obtained via these techniques were analyzed by using descriptive statistics, chi-square and one way ANOVA. The results revealed that majority (48%) of respondents initially planted M.stenopetala to consume its leaves as household food. The Chi-square test showed that there is no difference in reason for initial planting of M.stenopetala among wealth categories. However, the results revealed that there is difference in reason for primary planting among different age categories. Accordingly, households those in age group >61 (50%) planted it to protect fields on the farm land. Most farmers experienced positive environmental impacts from M.stenopetala, but few farmers had negative experiences which is the tree harbours birds which destroy other crops and it usually is associated with expansion of Monsonia angustifolia weed. However, therefore, it could be concluded that there is a great scope to improve the planting of M.stenopetala with suitable M.stenopetala approaches for maximizing income of the farmers.
The correlation between rs2501577 gene polymorphism and biliary atresia: a systematic review and meta-analysis
Authors: Li Tengfei , Ke Xingyuan , Zhang Yanran , Zhan Jianghua 
Published: April 2023

Abstract
Importance:
Multiple studies indicate a possible correlation between ADD3 rs2501577 and biliary atresia susceptibility; however, a conclusive determination has yet to be made.
Objective:
Investigate the role of ADD3 rs2501577 in biliary atresia susceptibility across diverse populations.
Data Sources:
The study protocol has been registered on PROSPERO, an international platform for systematic review registration (PROSPERO ID: CRD42023384641). The following databases will be searched until February 1, 2023: PubMed, Embase, Cochrane, CBM, Web of Science, and CNKI.
Study Selection:
Eight studies were selected from seven papers to assess the data. A total of 7,651 participants were included, consisting of 1,662 in the BA group and 5,989 in the NC group.
Data Extraction and Synthesis:
Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines were followed while conducting the systematic reviews and meta-analyses. Two authors independently assessed the quality of the included studies using the Newcastle–Ottawa Quality Assessment Scale. The significance of the pooled odds ratio (OR) was evaluated with a Z test, and statistical heterogeneity across studies was assessed using the I2 and Q statistics. Publication bias was assessed using Egger’s and Begg’s tests.
Main Outcome(s) and Measure(s):
The primary study outcome was the development of biliary atresia. Subgroup analysis was performed based on race, region, and assessment of Hardy-Weinberg equilibrium (HWE).
Results:
The studies indicate that the ADD3 rs2501577 susceptibility locus increases the risk of developing biliary atresia, regardless of allelic, homozygote, dominant, and recessive gene inheritance models. Furthermore, ADD3 has been found to be associated with apoptosis, cell cycle, and cell damage repair based on functional analysis.
Conclusions and Relevance:
The ADD3 rs2501577 polymorphic locus is associated with an increased risk of biliary atresia, particularly in Asian populations. This study recommends further investigation of the ADD3 rs2501577 locus in Asian populations to validate its role in the diagnosis of biliary atresia.
Seroprevalence of Hepatitis B Virus Infection is Associated with Male Circumcision among Senior High School Students in the Krachi Nchumuru District in the Oti Region of Ghana: A cross-sectional study
Authors: Nyambah Patrick K. , Adjei Richard , Sarfo Bismark 
Published: April 2023

Abstract
Background: There is paucity of hepatitis B virus (HBV) data among student populations although Ghana is HBV endemic. Screening and identification of factors associated with HBV transmission in schools will support the intervention in the elimination of the virus by 2030. This study assessed the seroprevalence and factors associated with HBV among students in two Senior High Schools in the Krachi Nchumuru District in Ghana.
Methods: Through descriptive cross-sectional design and simple random sampling technique, 300 first-year students were enrolled from selected Senior High Schools. Structured questionnaires were used to collect data on demographic and exposure factors while rapid test kit was used to detect HBV infections. Chi-square/Fisher exact test and multivariable logistic regression were performed to determine the association between the variables at a 95% confidence interval and 0.05 significant level.
Results:Seroprevalence of HBV was 14% (42/300) among the students. The prevalence was significantly (p=0.001) higher in males 19.4% (34/175) than females 6.4% (8/125). 77.7% (233/300) were aware of HBV infection. Males who were circumcised were 4 times more likely to be infected with HBV (AOR=4.09, 95%CI=1.82-9.19) (p=0.001) compared with those uncircumcised.
Conclusion: HBV screening and education on hygienic genital circumcision practices must be prioritized in endemic countries.
Regeneration of copper-loaded pine bark biochar using simultaneous bio-sulfide precipitation of copper
Authors: Bashir Misbah , Mohan Chander , Annachhatre Ajit P. 
Published: April 2023

Abstract

This research investigated adsorption of copper from aqueous solution onto the pine bark biochar, removal of adsorbed copper by bio-sulfide precipitation, and simultaneous regeneration of pine bark biochar adsorbent. A sulfidogenic reactor was established and operated under anaerobic conditions. During the sulfidogenic phase, COD:SO42− was gradually increased from 24:1 to 4:1. Use of sulfide-rich effluent from bio-sulfide reactor at neutral pH yielded above 99% copper removal from the aqueous solution. In the experiment's second stage, pine bark biochar was prepared through slow pyrolysis at 650 °C from pine bark residue that had a carbon content of 81% and a surface area of 368 m2/g. This biochar was then used in subsequent experiments. Initially, copper was adsorbed onto the biochar under neutral pH at contact time of 6 h. Maximum biochar adsorption capacity of 106 mg/g of copper was obtained. Finally, biochar was regenerated by precipitating the adsorbed copper as copper sulfide using sulfide-rich effluent from the sulfidogenic reactor. Complete recovery of adsorbed copper from biochar as copper sulfide precipitates were obtained was also confirmed by EDX-SEM analysis of biochar and precipitates. Regenerated biochar could be reused as an adsorbent in the subsequent adsorption cycle.