bluuebunny/crossref_metadata_2025_split huggingface dataset:
Serum connexin 26 as a potential marker of non-syndromic hearing loss
Authors: Moness Hend M. , Abdullah Noha M. , Amrousy Doaa El , Gamal Reham , Mohamed Hatem A. , Gomaa Mohammed A. , Hafez Shaimaa Moustafa , Mohamed Zamzam Hassan 
Published: January 2025

Abstract

Background
Hearing loss (HL) represents the most common form of sensory impairment in both children and adults. In children, genetic causes account for over 50%. The genetic causes of HL include multiple syndromes associated with HL. In non-syndromic hearing loss (NSHL), patients have only hearing impairment or loss. 35delG, 167delT are common mutations that lead to severe HL. The prevalence of these mutations varies according to region, population characteristics, and within the same region according to the genetic pools. There is currently no approval for laboratory investigations to diagnose non-syndromic hearing loss (NSHL) except few studies that are based on expensive genetic tests. This study evaluates the value of connexin 26 as a screening test for NSHL.

Methods
One hundred forty children participated in our study; they were divided into two groups. Seventy children with NSHL and 70 children as control group. All children were subjected to Audiometry, measurement frequency of 35delG, 167delT by PCR–RFLP, and connexin26 level by ELISA.

Results
We found that the connexin 26 level by ELISA was significantly lower in patients compared to controls (p = 0.0001). In addition, there was a significant negative correlation between connexin 26 levels and the degree of hearing loss by audiometry (r = − 0.403, p < 0.0001). In ROC curve analysis, serum levels of connexin 26 have sensitivity (94.3%) and specificity (100%). Measuring of connexin 26 by ELISA is more sensitive and specific than 35 del G mutation where sensitivity was 11.45 and 5.7% for both hetero and homo mutation.

Conclusion
Serum connexin 26 could be used as a good diagnostic marker to detect NSHL in children. It could be used as a potential marker for NSHL in newborns as it is more rapid and easily available than genetic study, especially in developing countries.
Idiopathic pulmonary fibrosis is a risk factor for cardiovascular disease: potential role of KL-6 and systemic inflammation
Authors: AbdelGhany Mohamed F. , Khaleel Waleed GamalEldin , Ahmed Asmaa Omar , Ahmed Ahmad BaheyElden , Bakkar Lamees M. 
Published: January 2025

Abstract

Background
Idiopathic pulmonary fibrosis (IPF) is a chronic, progressive, and fatal disease of the lungs. It is characterized by pulmonary and extrapulmonary comorbidities. So far, little is known as regards the prevalence of cardiovascular comorbidities in IPF patients. This study aims to investigate the prevalence of cardiovascular comorbidities in patients with IPF and correlate it with different radiological and laboratory indices of disease severity.

Methods
This prospective case–control study was performed on 134 IPF patients above 18 years. Diagnosis of IPF was based on diagnostic radiologic criteria addressed by ATS guidelines. Patients were recruited from the Pulmonology Department, Assiut University Hospitals, from June 2023 to June 2024. Cardiac comorbidities were assessed by ECG and echocardiography. All patients had C-reactive protein (CRP), Krebs von den Lungen-6 (KL-6), and lipid profile including cholesterol level, triglyceride level, HDL-cholesterol level, and LDL-cholesterol level measured. Patients were divided into two groups: IPF patients with cardiovascular comorbidities and IPF patients without cardiovascular comorbidities. Comparison between both groups as regards clinical, radiological, and laboratory criteria was carried out.

Results
The studied group consists predominantly of females (65%). Sixty out of the 134 documented IPF patients had cardiovascular comorbidities (44.7%), cardiomyopathy (mean ± SD: 43.63 ± 10.56), pulmonary hypertension (mean ± SD: 41.67 ± 15.32), ECG-ischemic changes (40.0%), and atrial fibrillation (13.3%). IPF patients with cardiovascular comorbidities vs. patients without had higher radiological HRCT total fibrosis score TFS (P value < 0.001). As regards laboratory serum biomarkers, the group with comorbidities showed significantly higher CRP, KL-6, cholesterol level, triglyceride level, and LDL-cholesterol level (P value < 0.001). Box plot analysis demonstrated significantly higher KL-6 serum level among IPF patients with cardiovascular comorbidities. Sensitivity 90.0%, specificity 94.6%, and accuracy 92.5% were associated with a cutoff value of KL-6 ≥ 299 for prediction of associated cardiovascular comorbidities among the studied IPF group.

Conclusions
Dyslipidemia and cardiovascular comorbidities were detected in a large group of IPF patients. These comorbidities were associated with a high HRCT TFS score. High serum levels of CRP and KL-6 were predictors of associated cardiovascular comorbidities in IPF.

Trial registration
ClinicalTrials.gov. NCT06539962.
Langerhans Cell Histiocytosis With Hypothalamic-pituitary and Bone Involvement: A Report of 2 Cases
Authors: Giraldi Erica A , Allen Jason W , McLemore Morgan L , Ioachimescu Adriana G 
Published: January 2025

Abstract
Case 1: A 31-year-old woman presented with secondary amenorrhea, polyuria, and polydipsia. Three years later, magnetic resonance imaging of the brain done for headaches found thickening of the pituitary infundibulum. Laboratory evaluation indicated central vasopressin deficiency, mild hyperprolactinemia, and central hypogonadism. Six months later, progression of the infundibular lesion was documented, now contacting the optic chiasm. Biopsy showed epithelioid histiocytes, chronic inflammation, and gliosis. On postoperative scan, a lesion in the parietal calvaria was identified, which was solitary on a bone scan. The patient received cytarabine for 12 months with resolution of infundibular and bone lesion on positron emission tomography-computed tomography scan 1 year later. Case 2: A 23-year-old man presented with polyuria, polydipsia, and unilateral tinnitus. Laboratory evaluation indicated vasopressin deficiency and central hypogonadism. External ear canal biopsy indicated an infiltrative lesion with eosinophils, small lymphocytes and histiocytes. Magnetic resonance of the brain revealed hypothalamic/infundibular and parietal and mastoid bone lesions; no other lesions were identified on positron emission tomography-computed tomography. Patient received cytarabine for 1 year with resolution of lesions, which was maintained during follow-up of 4 years. Although rare, Langerhans cell histiocytosis in adults should be considered in the appropriate clinical scenario. Multidisciplinary treatment is required.
Air–liquid interface culture combined with differentiation factors reproducing intestinal cell structure formation in vitro
Authors: Ogawa Isamu , Nakai Takaaki , Iwao Takahiro , Matsunaga Tamihide 
Published: January 2025

ABSTRACT
Reproducing intestinal cells in vitro is important in pharmaceutical research and drug development. Caco-2 cells and human iPS cell-derived intestinal epithelial cells are widely used, but few evaluation systems can mimic the complex crypt-villus-like structure. We attempted to generate intestinal cells mimicking the three-dimensional structure from human iPS cells. After inducing the differentiation of iPS cells into intestinal organoids, these were dispersed into single cells and cultured two-dimensionally. An air–liquid interface culture was used, with CHIR99021, forskolin, and A-83-01 used as key compounds. Long-term culture was also performed by adding Wnt3a, Noggin, and RSPO1, which are frequently used in organoid culture. The air–liquid interface culture combined several compounds that successfully induced the formation of a crypt-villus-like structure, which grew rapidly at around day 6. The expression of pharmacokinetic genes such as CYP3A4 was also enhanced. The intestinal stem cells were efficiently maintained by the addition of Wnt3a, Noggin, and RSPO1. We were able to construct a crypt-villus-like structure on cell culture inserts, which is considered a very simple culture platform. This structure had characteristics extremely similar to living intestinal tissues and may have a superior homeostatic mechanism.
Regulation of cell cycle in plant gametes: when is the right time to divide?
Authors: Simonini Sara 
Published: January 2025

ABSTRACT
Cell division is a fundamental process shared across diverse life forms, from yeast to humans and plants. Multicellular organisms reproduce through the formation of specialized types of cells, the gametes, which at maturity enter a quiescent state that can last decades. At the point of fertilization, signalling lifts the quiescent state and triggers cell cycle reactivation. Studying how the cell cycle is regulated during plant gamete development and fertilization is challenging, and decades of research have provided valuable, yet sometimes contradictory, insights. This Review summarizes the current understanding of plant cell cycle regulation, gamete development, quiescence, and fertilization-triggered reactivation.
Complexity and incoherence of self-narration versus self-esteem and perceived social support in healthy people and individuals with a diagnosis of psychotic disorders
Authors: Mazurek Martyna , Wajda Zbigniew 
Published: January 2025

Cel pracyCelem prezentowanego badania było porównanie autonarracji wśród osób z doświadczeniem psychozy i bez doświadczenia psychozy oraz korelacja tej zmiennej z samooceną i postrzeganym wsparciem społecznym w badanych grupach.MetodaGrupę badaną stanowiło 31 osób dorosłych z rozpoznaniem psychozy według ICD-10 (F20-29), a grupę kontrolną stanowiło 31 osób dorosłych bez rozpoznania psychiatrycznego. Bodźcem do stworzenia autonarracji uczestników była pierwsza część wywiadu autobiograficznego McAdamsa, wg którego określono spójność i złożoność wypowiedzi. Inne narzędzia wykorzystane w badaniu to kwestionariusz samooceny Rosenberga oraz skala postrzeganego wsparcia społecznego Zimeta i wsp.WynikiBadania wykazały, że obie grupy różniły się istotnie pod względem złożoności autonarracji (t= -3,185; p<0,05), grupy nie różniły się istotnie pod względem spójności. Ponadto zanotowano ujemną korelację między pomiarem spójności autonarracji, a wynkiem samooceny w grupie osób zdrowych (r= -,455; p<0,05).WnioskiAutonarracje osób z diagnozą zaburzenia psychotycznego okazały się mniej złożone niż autonarracje osób z grupy osób zdrowych, z kolei niespójność wypowiedzi ujemnie korelowała z samooceną, lecz tylko w grupie kontrolnej. Grupy - kliniczna i kontrolna nie różniły się pod względem niespójności autonarracji. Prezentowane badanie pokazuje możliwości wykorzystania metod jakościowych i wywiadu autobiograficznego do analizy języka osób chorych psychiczne oraz zdrowych i ich powiązanie z cechami psychologicznymi. Zarówno procedura badania jak i uzyskane wyniki mają swoje ograniczenia, które szczegółowo omówiono w artykule.
Pseudomonas mendocina bacteraemia secondary to cellulitis – a report and brief series of cases
Authors: Reis Jorge , Carmo Francisca , Soares Inês , Salvado Catarina , Fidalgo Mariana 
Published: January 2025

Background: Pseudomonas mendocina is a rare cause of disease in humans with few cases described, but known to cause severe infections, mainly endocarditis and bacteraemia. Case description: An 86-year-old Caucasian male, with several vascular risk factors (type 2 diabetes, hypertension, dyslipidaemia, coronary heart disease and heart failure), who presented long-standing lower limb phlyctens for which he was being treated, developed lower limb cellulitis. When observed in the Emergency Department, he was septic and was admitted for treatment and surveillance. Blood cultures were positive for P. mendocina. He was treated with antibiotics and recovered, and was discharged home. Conclusion: Damage to the skin barrier as in cellulitis provides a point of entry for P. mendocina, a microorganism that lives in soil and water. The source of contamination is rarely identified, but immunocompromised people present a higher risk of infection and severe disease. The antibiotic susceptibility profile is different from the more common Pseudomonas aeruginosa, with fewer known resistances. Albeit rare, this is thought to be an underreported infection that clinicians should be aware of.
A guide in undertaking core clinical supervision: Supervisors' experiences in implementing core clinical supervision in general practice
Authors: Goode Sarah , Hodge Gemma , Cross Debbie 
Published: January 2025

Background Many practitioners have highlighted the value in being given protected time to undertake supervision practice as it offers the opportunity of sharing knowledge and provides feedback around good clinical practice leading to high quality patient care delivery ( Rothwell et al, 2021 & Goode et al, 2024 ). As healthcare policies evolve, supervision practices can adapt and become embedded as part of the regulatory requirement ( NHS England, 2022 ). However, many practitioners struggle to either embed and/or sustain core clinical supervision practices. If left to drift a workforce may become poor functioning and non-concordant with the latest regulations and standards in primary care. Therefore, general practice practitioners require guidance and support to be increasingly creative and resourceful whilst managing clinical demands. Methodology/Methods An explorative hermeneutic phenomenology approach, using semi structured interviews was utilised to explore themes and experiences around implementing and facilitating core clinical supervision in practice. Findings Four key themes arose from the study. Identifying resources needed to implement core clinical supervision; Leading and managing clinical sessions; professional and personal development and quality improvement indicators. Recommendations and Conclusion Several recommendations are offered from the study to initiate and embed core clinical supervision in practice with emphasis on quality indicators, utilising some change management strategies and ensuring core supervision is sustainable in practice.
The re-emergence of the peripatetic educator in general practice
Authors: Cross Debbie , Hodge Gemma , Barwick Claire 
Published: January 2025

Peripatetic nursing roles have evolved due to need of flexibility to manage a workforce which aligns with any form or need for organisational change that involves a people strategy. Background Recently, the re-emergence of the peripatetic educator role in primary care has appeared through funding from NHS England to address the gap of practice assessors, particularly in primary care in accordance with The Ten Point Plan for General Nurse Practitioners (2021) has been applied at scale across the Humber and Yorkshire regions. Long standing evidence has justified that student nurses and staff can develop their knowledge and skills utilising the supervisory mechanisms to enable quality supervision and assessment within clinical environments ( Knight et al 2021 ). Methods A reflection on action process was utilised from peripatetic educators' reflections to investigate the quality and value of introducing a peripatetic educator to support and engage with student nurses and their supervisors and assessors in primary care networks (PCNs). Outcomes and Effectiveness The introduction of the peripatetic educator in general practice has had a positive impact in addressing general practice placements, specifically to enhance skill acquisition through nurse led clinics addressing key Quality and Outcomes Frameworks (QOF's); broaden placement capacity and complete proficiency assessments in a timely and successful way, by applying the long arm supervision process ( Karban 1999 ). Implications and Summary Peripatetic educators can cement the required consistency and proficiency in clinical placement learning and assessments by bridging gaps between primary care networks and universities; maximise the student tariff and provide high quality clinical placements within general practice.
Strategies to promote cervical screening for an aging demographic: a global insight
Authors: Mitchell Claire , Carlin Alexandra 
Published: January 2025

Claire Mitchell and Alexandra Carlin use studies from around the world to explain how cervical screening can be boosted, and the role primary care nurses can play in doing so. Cervical screening coverage, in the United Kingdom (UK), has been steadily declining year-on-year. Concurrently, the Human Papillomavirus (HPV) vaccination schedule excluded women born before 1990, therefore there is growing need to promote screening to those who were ineligible. Local data may help inform practices to identify non-attenders and allow development of targeted strategies to improve screening attendance with aim to meet the national target of 80%. NHS England aims to eradicate cervical cancer by 2040, however, much remains to be done to achieve this goal. Innovations such as self-sampling may be a vital tool to promote screening. This article will explore global targets, and the lessons learnt from countries with higher success rates, than the UK, in eradicating cervical cancer.
Higher magnesium depletion score increases the risk of all‑cause and cardiovascular mortality in US adults with diabetes
Authors: Zhang Hao , Kuang Liping , Wan Qiang 
Published: January 2025

Background
Both dietary magnesium and serum magnesium are associated with the prognosis of diabetic patients. However, the impact of the magnesium depletion score (MDS), which assesses systemic magnesium deficiency, on the prognosis of diabetic patients remains unclear. This cohort study aims to explore the potential association between the MDS and all-cause and cardiovascular mortality in diabetic patients.

Methods
In this study, we analyzed data from 5,219 diabetic individuals from National Health and Nutrition Examination Survey (NHANES) 2003–2018. Participant mortality information was sourced from the National Death Index records. MDS was divided into lower MDS (0–1 points), middle MDS (2 points), and higher MDS (3–5 points) groups. Weighted multivariable Cox regression was utilized to explore the potential association between MDS and mortality in diabetic patients. Stratified analyses and sensitivity analyses were employed to validate the robustness of our findings.

Results
Among the 5,219 participants included in this study, 1,212 experienced all-cause mortality, and 348 experienced cardiovascular mortality. Weighted multivariable Cox regression indicated that higher MDS was strongly linked to a heightened risk of mortality in all models, including the fully adjusted model (all-cause mortality: HR = 1.58, 95% CI: 1.20–2.08; cardiovascular mortality: HR = 1.92, 95% CI: 1.28–2.88). In the stratified analysis, we found that the association between MDS and all-cause mortality was stronger among individuals aged <60 years. No significant differences were found in the relationship between MDS and mortality within other subgroups. In the sensitivity analyses, our results remained robust.

Conclusions
An increase in MDS is significantly correlated with a higher risk of all-cause and cardiovascular mortality in diabetic patients. The risk of all-cause mortality was higher in diabetic patients aged <60. Early monitoring and management of MDS, as well as optimizing magnesium nutritional status, may benefit diabetic patients.
Szkolnictwo baletowe w Polsce i na ziemiach polskich – zarys dziejów
Authors: Sadowska Katarzyna 
Published: January 2025

The study aims to outline the history of ballet education in Poland and on Polish lands based on the method of analysis and criticism of literature within the qualitative research paradigm. Research on the research problem expressed in the question: “What is the outline of the history of ballet education in Poland?” belongs to the qualitative research paradigm. The topic of the history of ballet education in Poland can be considered important because although ballet has been present in Polish culture since the 16th century, in the Polish publishing market there are still relatively few studies on artistic education in the field of dance, or they are outdated. To gradually change this situation, the study’s authors reflected on the key events in the history of Poland in shaping the tradition of professional education in the profession of dancers, expressing the hope that this reflection will contribute to a broader discussion and an inspiration for further research analyses.
Previously Unidentified Hurrian Words in Nuzi Texts
Authors: Planelles Albert 
Published: January 2025

Abstract
This article gathers a number of Hurrian words that can be found in Nuzi texts and that were not identified as such by previous research. Various attempts to understand these words within the framework of the Akkadian language have resulted in different, often diverging, suggestions for translation. Albeit still imperfect, the knowledge of the Hurrian language has increased significantly over the last few decades, thus allowing us to identify these words as belonging to Hurrian. Although in some cases their meanings remain unclear, the words gathered here slightly enhance the knowledge of the Hurrian lexicon and the distribution and number of attestations of some forms.
It is a pleasure to dedicate this short article to the memory of Johannes Friedrich. May it be a token of my admiration for such a remarkable scholar.
The effect of the difference in female body shapes on clothing fitting
Authors: Penko Tadeja , Rudolf Andreja 
Published: January 2025

Abstract
The aim of this study was to determine the frequency of body shapes of the studied group of women using different body shape classification methods. The objective was to investigate the fit of clothing for the average body shapes identified. Forty female participants from Slovenia, aged 20–30, were included in the analysis. Body shapes were classified using three methods: Visual assessment of body shape (VABS), female figure identification technique (FFIT), and body shape classification method (BSCM). For each classification method, ANOVA tests were performed to determine whether there were statistically significant differences in body measurements across the identified body shapes. A comparison of body shapes was carried out and also visualised by simulating 3D body shapes. The fit of clothing to body shapes was analysed with the help of virtual 3D prototyping. Four characteristic body shapes have been identified, namely triangle (pear, spoon), hourglass, rectangle and inverted triangle. The VABS method differs slightly from the other two (FFIT, BSCM), which give very similar results. When applying the FFIT and BSCM, the triangle body shape is the most representative, followed by the hourglass shape, the rectangle and the inverted triangle shape. Simulating the fit of the basic dress pattern design on 3D body shapes using the best match of dress size shows that the dress fits the hourglass shape best, while the fit for the other three shapes is inadequate. The latter indicates the need to make most of the garments for the triangle body shape and a corresponding number of garments for the hourglass and rectangle body shapes to ensure the correct fit of the garments.