bluuebunny/crossref_metadata_2025_split huggingface dataset:
Applying RNA-Seq to investigate the transition into a Viable But Non Culturable State (VBNC) for the intracellular pathogen, Francisella tularensis LVS.
Authors: Cantlay Stuart , Garrison Nicole , Patterson Rachelle , Primerano Donald , Fan Jun , Horzempa Joseph 
Published: June 2023

Many species of bacteria, under conditions of stress or nutrient limitation, enter a state of dormancy referred to as viable but non-culturable (VBNC). VBNC bacteria persist in the environment, are difficult to detect and identify by many standard laboratory methods and can be altered in their susceptibility to antibiotics. Entry into the VBNC state is often accompanied by morphological changes; however, the mechanisms underlying this are poorly understood. Francisella transitions rapidly and spontaneously to a VBNC state, and therefore has the potential to be an excellent model organism for the study of this phenomenon. To investigate the transcriptome of VBNC F. tularensis LVS we extracted RNA from culturable and VBNC cells and carried out an RNA-Seq analysis using both Long-read Nanopore and Illumina Sequencing. Differentially expressed genes (DEGs) were identified using a DE-Seq pipeline. Over 300 genes were significantly upregulated and ~100 genes were down regulated in VBNC cells. Amongst the upregulated genes were some involved in the transport of metals or small molecules, and we have also identified putative transcriptional regulators that may be master controllers of the VBNC process. Our data represents the first transcriptomic analysis of F. tularensis LVS as it transitions into the VBNC state. Identifying genes that are involved in this transition is a critical first step in understanding the mechanisms that drive F. tularensis LVS into the VBNC state and will help us identify factors that allow the resuscitation of these bacteria. This will have important implications for understanding both environmental persistence and pathogenicity of Francisella species. (Supported by NIH Grant P20GM103434 to the West Virginia IDeA Network for Biomedical Research Excellence)
Karst Terrain Promotes Thermal Resiliency in Headwater Streams
Authors: Kessler Karmann , Rogers Karli , Marsh Charles , Hitt Nathaniel 
Published: June 2023

The response of stream ecosystems to climate change will depend in part on groundwater processes that reduce the sensitivity of streams to atmospheric conditions. We investigated the thermal sensitivity of streams across a gradient of groundwater inputs defined by karst terrain (carbonate parent materials) in the headwaters of the Potomac River basin in eastern North America. We collected stream temperature data and quantified thermal sensitivity for 30 sites from the relationship between daily mean water and air temperatures. Our analysis demonstrates that thermal sensitivity is lower for streams in karst terrain than elsewhere, and that the effect of karst terrain is more important than effects of elevation or basin size in this regard. Our study indicates the importance of karstic groundwater for stream thermal resiliency and suggests the importance of riparian vegetation for maintaining stream temperatures elsewhere. Our study also provides a simple and rapid method for climate change research that can be implemented in conjunction with watershed organizations and citizen science networks.
Dendroecological Potential of Appalachian Riverscour Woodland Trees
Authors: DePinho Haidyn , Saladyga Thomas 
Published: June 2023

Appalachian riverscour woodlands are intermittently flooded areas along high-energy rivers characterized by exposed bedrock and poorly developed sandy soils, high plant diversity, and scattered and stunted trees. These sites can be negatively impacted by dam construction and alterations to river hydrology, trampling associated with riverside recreation activities, and non-native species. In West Virginia, little is known about the development of riverscour woodlands or the growth and hydroclimate sensitivity of associated tree species. In this exploratory study, our objectives were to evaluate our ability to crossdate riverscour woodland trees (i.e., assign accurate calendar years to annual growth rings) as well as determine stand structure and tree establishment patterns at one site along the New River in Fayette County, West Virginia. In addition, for two species, Ulmus americana and Diospyros virginiana, we examined annual growth trends and relationships between tree growth and monthly hydroclimate, including streamflow, precipitation, and Palmer Drought Severity Index (PDSI). Seventy-three percent of all ring-width measurement series were crossdated, but results were mixed across species. Most trees were less than 50 years old and no trees established before the late 1940s. Both U. americana and D. virginiana were highly sensitive to April–August hydroclimate, but differed in response to previous year moisture availability. These findings provide new direction for the use of riverscour woodland trees in future dendroecological studies.
A Preliminary Study of Cobalt Solvent Extraction from Nickel Sulphate Solution using Organic Extractant-PC-88A
Authors: Subagja Rudi , Rohman Arief Dwi , Milandia Anistasia , Oediyani Soesaptri , Setiawan Iwan 
Published: June 2023

In present study, a solvent extraction experiment has been done to separate cobalt from the nickel sulfate solution by using 2-ethythexyl phosponic acid mono-2-ethylhexyl ester (PC-88A) as extractant. The experiment was carried out on a laboratory scale by using a separating funnel to extract cobalt from the nickel sulfate solution with PC88A. The mixed solution was shake in separating funnel for a specified period of time, and after the solvent extraction experiment was finished the organic phase PC88 was separated from the nickel sulfate solution by decantation. The nickel and cobalt content in the aqueous nickel sulfate solution were then analyzed using Atomic absorption spectrophotometry (AAS). In this experiment, the variable for experiments were covering solution pH from 2 to 6, shaking time from 30 minutes to 120 minutes, shaking speed from 20 revolutions per minute (rpm) to 80 rpm, and the volumeratio of aqueous to organic phase (A:O ratio) was from 1:1 to 1:4. The effects that experimental variables to the cobalt extraction were observed in this experiment. The result of experiment at room temperature, solution pH 5, shaking speed 60 rpm, shaking time 90 minutes, A:O ratio 1:4 and concentration of PC- 88A 40% show 97.21% of cobalt can be extracted by PC-88A from nickel sulfate solution, thererfore it was necessary to conduct two stage extraction process to extract 100% of the cobalt from the nickel sulfate solution.
Synthesis of Tin Oxide Nanocrystallites with Various Calcination Temperatures using Co-Precipitation Method with Local Tin Chloride Precursor
Authors: Panthoko Norbert Egan Christo , Septiningrum Fairuz , Yuwono Akhmad Herman , Nurhidayah Eka , Maulana Fakhri Akbar , Sofyan Nofrijon , Dhaneswara Donanta , Lalasari Latifa Hanum , Arini Tri , Andriyah Lia , Firdiyono Florentinus , Ardianto Yahya Winda , Pawan Ria Wardhani 
Published: June 2023

Indonesia is one of the largest tin metal producers in the world, and one of its derivative products is tin chloride (SnCl4). This material has been used as a raw ingredient for the production of organotin compounds such as methyltin mercaptide for PVC (polyvinyl chloride) plastic industry as a heat stabilizer. On the other hand, this precursor can be used to synthesize SnO2 nanomaterials, which have other strategic potentials, including photocatalysts and solar cell applications. In this study, the synthesis of SnO2 nanocrystallites was carried out using a local tin chloride precursor via the co-precipitation method, followed by a calcination process at temperatures of 300, 400, 500, and 600 °C, for further usage as an ETL (electron transport layer) in a PSC (perovskite solar cell) device. The basic properties characterization was carried out using XRD (X-ray diffraction), ultraviolet-visible (UV-Vis) spectroscopy, and SEM (scanning electron microscopy), while the photocurrent-voltage (I-V) curve photovoltaic performance of the device was performed using a semiconductor parameter analyzer. The characterization results showed that increasing the calcination temperature from 300 to 600 °C increased the average crystallite size from 1.19 to 13.75 nm and decreased the band gap energy from 3.57 to 3.10 eV. The highest PCE (power conversion efficiency) was obtained from the device fabricated with SnO2 nanocrystallites calcined at a temperature of 300 °C, which was 0.0024%. This result was obtained due to the highest transmittance of this sample as compared to others; the higher the transmittance, the better the performance of the ETL, which in turn increased the overall efficiency of the PSC
El uso de medios interactivos para el desarrollo de la inteligencia emocional
Authors: Mendoza -Zambrano María Guadalupe , Meza-Montes Jéssica Katherine , Meza-Montes Fernando Javier 
Published: June 2023

La forma de enseñar es transcendente y los estudiantes aprenden diferente; la innovación promueve un desempeño favorable en los salones de clases y contribuye a tener mejor eficiencia y eficacia en los estudiantes. El objetivo de este estudio es analizar el uso de los medios interactivos para desarrollar la inteligencia emocional de los estudiantes en el ámbito educativo. En términos de metodología, se utilizó un enfoque cuantitativo, no experimental y descriptivo. Se emplearon métodos teóricos, empíricos y matemáticos o estadísticos, que permitieron a los investigadores acercarse al objeto de análisis y recopilar información de manera directa y precisa. La muestra del estudio constó de 45 estudiantes, representando la población total.
Según el diagnóstico realizado, se evidenció que la educación emocional en entornos educativos desde temprana edad favorece el desarrollo emocional, el aprendizaje y, por ende, el éxito académico de los estudiantes en todos los niveles educativos. Se concluye que para ser emocionalmente competentes se requiere práctica continua, por lo que este tipo de aprendizaje debe iniciarse en los primeros años de vida y estar presente durante toda la vida; la aplicación de un sistema de actividades desarrollara la inteligencia emocional en los estudiantes mejorando los hábitos de estudios obteniendo resultados académicos sobresalientes.
On the situation of the national economy during the Antonescu regime (september 1940 - august 1944)
Authors:   Academy of Romanian Scientists, Drecin Mihai D. ,   University of Oradea, Romania.
Published: June 2023

The present paper provides a brief presentation of the evolution of Romania’s national economy in the period September 1940 - August 1944, as indicate the published bibliography and unpublished archive documents of the time. It addresses the national economy and the policy of “Romanianization”, aimed at transferring the industrial and commercial enterprises and assets owned by Jews, Greeks, Armenians and Italians or Romanian citizens belonging to the aforementioned ethnic groups, to state ownership. Command of these economic enterprises was given by the state to the so-called "commissioners of Romanianization". They had to organize the economy in order to meet the needs of the anti-Soviet front. In reality, the Romanian state wanted to protect the local capital, regardless of the ethnicity of the owner/owners, from the offensive of the German capital which sought to regain its privileged and dominant positions from 1914-1916. The economy of the Antonescu regime, rid of the legionary Romanianization commissioners, who sought only to enrich themselves in connivance with the Jewish owners, pursued a strict planning of the war economy, in order to obtain maximum quantities of products and good quality for the supply of the ”Eastern Front” and of the cities, and to counter the economic sabotage set up by the Communist Party of Romania, which was illegal and totally subordinated to the interests of the USSR.
IMPLEMENTASI KEWIRAUSAHAAN SYARIAH SANTRI DI SEKOLAH MENENGAH KEJURUAN PONDOK PESANTREN MODERN SAHID MARYAM AFIFAH
Authors: Afifah Maryam 
Published: June 2023

Penelitian ini bertujuan untuk mengetahui bagaimana implementasi kewirausahaan santi di SMK Sahid serta bagaimana menanamkan nilai-nilai Islami pada praktek kewirausahaan di SMK Sahid. Penelitian ini menggunakan metode kualitatif dengan pendekatan penelitian lapangan (field research) yang disajikan secara deskriptif. Pengumpulan data dalam penleitian ini dilakukan dengan cara wawancara, observasi, dan dokumentasi terhadap 10 narasumber yang terdiri dari 5 pengurus SMK Sahid dan 5 Santri. Hasil penelitian menunjukan bahwa kewirausahaan santri yang diterapkan di SMK Pondok Pesantren Sahid Modern bertujuan untuk menumbuhkan jiwa wirausaha, jiwa mandiri, serta mempersiapkan santri untuk memanfaatkan setiap peluang bisnis dengan menciptakan produk wirausaha dan menjualnya di acara-acara sekolah, seperti festival sekolah, penerimaan siswa baru, atau kegiatan lainnya. Produk yang dihasilkan oleh santri SMK Sahid meliputi produk kuliner seperti jajanan pasar, masakan continental dan oriental serta masakan kemasan yang tahan lama seperti kue kering dan keripik. Dalam menanamkan nilai-nilai Islami pada praktek kewirausahaan di SMK Pondok Pesantren Modern Sahid, Bahan baku halal dan nilai kejujuran serta amanah merupakan hal yang penting dan harus diutamakan dalam setiap kegiatan, termaksud aktivitas kewirausahaan. Selaras dengan apa yang diajarkan dan dicontohkan oleh Nabi Muhammad SAW.
HUBUNGAN JENIS JAJANAN DENGAN TUMBUH KEMBANG ANAK USIA 3-5 TAHUN DI TAMAN KANAK-KANAK ZAINUL HASAN GENGGONG PROBOLINGGO
Authors: Sary Yessy Nur endah 
Published: June 2023

Latar belakang: Pertumbuhan dan perkembangan anak usia dini bergantung kepada kualitas dan kuantitas dari makanan yang diberikan orang tua. Permasalahan gizi yang sering dialami anak usia dini antara lain kegemukan atau obesitas, gagal tumbuh, anemia, karies gigi dan kecacingan. Tujuan : menganalisis hubungan jenis jajajan dengan tumbuh kembang anak usia dini (3-5 Tahun) di taman kanak-kanak Zainul Hasan Probolinggo. Metode : Desain penelitian cross sectional. Populasi dalam penelitian ini adalah semua anak usia dini (3-5 tahun) di TK Zainul Hasan, Desa Pajarakan, Kabupaten Probolinggo berjumlah 67 orang. Teknik simple random sampling. Sampel penelitian 71 anak usia dini. Alat ukur yaitu lembar observasi dan kuesioner Deteksi Dini Tumbuh Kembang (DDTK). Analisis data univariat yang digunakan adalah distribusi frekuensi. Analisis data bivariat yang digunakan adalah chi square. Hasil : Terdapat keterkaitan antara jenis jajanan dan tumbuh kembang anak usia dini memiliki hubungan yang sangat signifikan, hal tersebut dibuktikan dengan hasil analisis statistik yang memiliki nilai P Value sebesar 0,017 < α 0,050 Kesimpulan : Ada hubungan antara jenis jajanan dengan tumbuh kembang anak usia 3-5 tahun di Taman Kanak-kanak Zainul Hasan Probolinggo.
NALISA EFISIENSI PENGGUNAAN TEMPAT TIDUR BERDASARKAN INDIKATOR DEPARTEMEN KESEHATAN DI RUANG RAWAT INAP RS BHAYANGKARA SARTIKA ASIH TK II BANDUNG PERIODE TW 1 2021
Authors: Syaidah Eka Winda , Wahab Syaikhul 
Published: June 2023

Dalam mengolah efisiensi rawat inap dibutuhkan unit rekam medis yang mampu menunjang tercapainya tertib adminstrasi. Kriteria atau parameter tertentu di butuhkan untuk menentukan apakah tempat tidur yang tersedia telah berdaya guna dan berhasil. Parameter tersebut salah satunya adalah Bed Occupancy Rate (BOR ), Turn Over Interval (TOI), dan Bed Turn Over (BTO). Tujuan dari penelitian ini adalah untuk mengetahui gambaran umum tingkat efisiensi pendayagunaan tempat tidur berdasarkan indikator departemen kesehatan di Rumah Sakit Bhayangkara TK II Sartika Asih Bandung metode penelitian yang dilakukan adalah metode deskiptif populasi dalam penelitian ini adalah data sensus harian rawat inap (SHRI) triwulan I Tahun 2021 Sampel yang digunakan adalah total sampling yaitu sensus harian rawat inap (SHRI) semua ruangan triwulan 1 Tahun 2021 instrumen penelitian berupa wawancara dan observasih hasil dari triwulan I 2021 nilai BOR 50,60 % TOI 4 BTO 10.4
Editorial E-locução, 23ª edição/2023
Authors: DO COUTO BARBOSA JOSÉ EDUARDO 
Published: June 2023

Prezados leitores,A Revista E-Locução tem se tornado cada vez mais um instrumento de promoção do desenvolvimento científico nacional e internacional. A partir de 2017, ela passou a ser classificada com QUALIS CAPES B3 na Área de Administração, Ciências Contábeis e Turismo, QUALIS C - Áreas de Educação e Letras e QUALIS B5 - Engenharias III e IV, e em 2018, alcançamos QUALIS C na Área de Linguística eLiteratura...
New record and new species of Laubierpholoe Pettibone, 1992 (Annelida, Sigalionidae) from the soft bottom of submarine caves near Marseille (Mediterranean Sea) with discussion on phylogeny and ecology of the genus
Authors: Zhadan Anna , Tzetlin Alexander , Vortsepneva Elena , Neretina Tatiana , Chevaldonné Pierre 
Published: June 2023

A new species of Laubierpholoe Pettibone, 1992 (Annelida, Sigalionidae), Laubierpholoe massiliana Zhadan sp. nov., was found in two submarine caves near Marseille (France). This is the first record of the genus in the Mediterranean Sea. The new species differs from congeners by inhabiting soft sediments instead of having an interstitial lifestyle and by several morphological characters: the ventral tentacular cirri slightly shorter or of similar length to the dorsal tentacular cirri, the presence of bidentate neurochaetae, the body length, and the number of segments. Molecular phylogenetic analysis using 18S rRNA and 28S rRNA sequences confirmed that the new species belongs to the genus Laubierpholoe, as well as the monophyly of the genus. The ecology of the new species and its adaptation to the cave-dwelling lifestyle are discussed. An identification key for all known species of Laubierpholoe is provided.
Hypertrophic Lichen Planus: An Up-to-Date Review and Differential Diagnosis
Authors: Whittington Carli P. , Saleh Jasmine S. , Bresler Scott C. , Patel Rajiv M. 
Published: June 2023

Context.—
Hypertrophic lichen planus (HLP) is a variant of lichen planus that can be difficult to diagnose based on histopathologic features alone. Thus, patient clinical history and clinicopathologic correlation are essential considerations to make the correct diagnosis.

Objective.—
To discuss the clinical and histologic presentation of HLP and provide a thorough review of commonly encountered mimickers in the differential diagnosis.

Data Sources.—
Data were derived from a literature review, personal clinical and research experiences, and a review of cases in the archives of a tertiary care referral center.

Conclusions.—
In general, HLP involves the lower extremities and is characterized by thickened, scaly nodules and plaques that are often pruritic and chronic in nature. HLP affects both males and females and is most common in adults 50 to 75 years of age. Unlike conventional lichen planus, HLP tends to have eosinophils and classically displays a lymphocytic infiltrate most concentrated around the tips of rete ridges. The differential diagnosis for HLP is broad and encompasses numerous entities in many different categories, including premalignant and malignant neoplasms, reactive squamoproliferative tumors, benign epidermal neoplasms, connective tissue disease, autoimmune bullous disease, infection, and drug-related reactions. Therefore, a high index of suspicion must be maintained to avoid a misdiagnosis and potential inappropriate treatments.
Efforts to Increase Public Knowledge About Health and the Use of Gadgets for Digital Marketing at RW 01 Babakan Ciparay Bandung City
Authors: Yogisutanti Gurdani , Ardayani Tri , Ekawati Dian , Theresia Ngadiman Susanna , Juliyanti  , Manan Srihesty , Adhy Prasetyo Yunus , Dwi Utari Ardhini , Harun Iriyanti 
Published: June 2023

Community service activities carried out by Working Group 1 Immanuel Bandung Health Institute in RW 01 Babakan Ciparay Village, Babakan Ciparay District, Bandung City. Community service aims to Increasing public knowledge about health and utilization gadgets for digital marketing at RW 01 Babakan Ciparay Bandung City. Activities involving 8 lecturers from the S1 Nursing, S1 Public Health, S1 Nutrition, D3 Nursing and D3 Hospital Service Management (MPRS) study programs. There were 10 students involved, consisting of 2 students from the S1 Kesmas Study Program, and 8 people from the D3 MPRS Study Program. Community service methods are carried out by means of counseling, lectures and questions and answers, using videos, posters, stickers, and direct practice. The media used are televisions, and laptops to display material in the form of power points. The material presented to the community was carried out in 2 stages and took place at the house of the head of RW 01 Babakan Ciparay. Phase 1 was held on Wednesday, September 8, carried out by 2 lecturers to deliver material on the use of gadgets in the era of digital marketing, family mental health and video screenings on the prevention of COVID-19, hypertension and diabetes mellitus. The second phase was held on Tuesday, 27 September 2022. The material presented includes: mental health of pregnant women and the importance of eating fruits and vegetables by means of lectures using power point media. Material on hypertension is delivered directly through lectures and questions and answers. Material on handling cardiac arrest at home, carried out with direct practice to the community. Media used for learning using mannequin aids. In addition, posters about COVID-19 prevention and stickers on how to sort waste were also given. The results of community service can increase public knowledge about health in general and the use of gadgets for digital marketing. The constraints of this community service activity have not been evaluated in a structured manner using questionnaires. The suggestion that can be recommended is that the implementation of community service be carried out in the same location with different materials, and that IKI should have a target area. Community service activities can then be continued with research first to find out the problems that exist in the community.
TIME AS DISTENTIO ANIMI ACCORDING TO SAINT AUGUSTINE
Authors: Simplesius Sandur  
Published: June 2023

Apakah waktu? Apakah waktu itu ada? Bagaimana mengukurnya? Pertanyaan-pertanyaan yang luput dari perhatian kita tetapi Agustinus telah menjawabnya. Jelas waktu itu ada dan ia ada karena ia “lewat” dalam hidup kita. Bagi Agustinus waktu itu adalah distentio animi atau penggelembungan jiwa. Disebut “pengegelembungan” karena waktu adalah suatu dialektika antara 3 hal: memori, perhatian dan harapan. Penggelembungan jiwa membawa masa lalu dan yang akan datang pada saat ini yang sedang lewat. Masa lalu, masa depan dan sekarang selalu hadir dalam jiwa. Dalam masa lalu ada memori, dalam masa depan ada harapan dan saat ini ada perhatian dan ketiganya adalah aktivitas dari jiwa. Waktu itu adalah suatu dialektika tiga hal “sekarang”: sekarang tentang hal-hal masa lalu, sekarang tentang hal-hal sekarang dan sekarang tentang hal-hal yang akan datang.
THE LITURGY ACCORDING TO THE DIDACHE
Authors: Angelo Luciani Moa Dosi WODA  
Published: June 2023

Tidak dapat disangkal bahwa Didache yang dikenal sebagai “ajaran Tuhan kita Yesus Kristus melalui tradisi para rasul” menunjukkan kesaksian otentik Gereja universal sejak pertengahan abad pertama hingga saat ini. Kendati, dijumpai pelbagai macam pertanyaan berkaitan dengan ajaran iman dan moral Kristiani, sesungguhnya Didache menunjukkan intisari iman Kristiani yang bersumber dari misteri Yesus Kristus dan Tradisi Apostolik. Itulah sebabnya, betapa pentingnya memahami perkembangan ajaran iman Kristiani dalam Tradisi Gereja perdana hingga saat ini. Refleksi iman Kristiani menunjukkan bahwa tak ada berubah dalam iman Kristiani, hanya cara kita mengungkapkannya yang berkembang sesuai dengan perkembangan zaman. Penghayatan liturgi Gereja, terutama Sakramen Inisiasi sejak era Didache hingga saat ini tetap, konsisten, dan dinamis, namun Tradisi mencatat adanya perkembangan yang signifikan dan kekayaan ritus liturgi Gereja sepanjang zaman.