bluuebunny/crossref_metadata_2025_split huggingface dataset:
Interaksi Jenis Mikoriza Dan Beberapa Varietas Cabai (Capsicum annuum L.) Terhadap Pertumbuhan Dan Hasil Pada Tanah Ultisol Aceh Besar
Authors: Hermawan Agus , Jumini Jumini , Syafruddin Syafruddin 
Published: May 2023

Abstrak. Tujuan penelitian ialah mengetahui interaksi antara jenis mikoriza dengan varietas terhadap pertumbuhan dan produksi cabai merah. Penelitian menggunakan Rancangan Acak Kelompok faktorial 3 x 3 dengan 3 ulangan, sehingga terdapat 27 unit percobaan. Adapun faktor yang amati ialah jenis mikoriza, terdiri dari tiga taraf yaituMikoriza Glomus mosseae, Mikoriza Gigaspora sp. dan Mikoriza campuran (Glomus mosseae + Gigaspora sp.). Perlakuan varietas, terdiri dari 3 taraf yaitu Laju F1, Lado F1 dan Baja F1. Hasil penelitian menunjukkan bahwa interaksi antara jenis mikoriza dengan beberapa varietas cabai merah sangat nyata terhadap diameter batang 15 HSPT dan jumlah buah pertanaman serta interaksi nyata terhadap tinggi tanaman 15 HSPT, berat kering keseluruhan tanaman dan berat basah akar. Kombinasi perlakuan yang lebih baik terhadap pertumbuhan dan hasil tanaman cabai merah terdapat pada jenis mikoriza Gigaspora sp. dengan varietas Laju F1. Interaction of Mycorrhizal Types and Several Chili Varieties (Capsicum annuum L.) on Growth and Yield in Ultisol Aceh Besar Abstract. The research objective was to determine the interaction between mycorrhizal species and varieties on the growth and production of red chilies. The study used a 3 x 3 factorial randomized block design with 3 replications, so there were 27 experimental units. The observed factor is the type of mycorrhiza, consisting of three levels, namely Mycorrhizal Glomus mosseae, Mycorrhizal Gigaspora sp. and mixed mycorrhiza (Glomus mosseae + Gigaspora sp.). The variety treatment consisted of 3 levels, namely F1 Laju, F1 Lado and F1 Baja. The results showed that the interaction between mycorrhizal species and several varieties of red chili was very significant on stem diameter at 15 days after transplanting (DAT) and the number of fruit planted as well as a significant interaction on plant height at 15 DAT, dry weight of whole plant and fresh weight of roots. The better combination of treatments on the growth and yield of red chili plants was found in the mycorrhizal species Gigaspora sp. with the F1 Laju variety.
Novel Oleanolic Acid-Phtalimidines Tethered 1,2, 3 Triazole Hy-Brids As Promising Antibacterial Agents: Design, Synthesis, In Vitro Experiments and In Silico Docking Studies
Authors: Lahmadi Ghofrane , Horchani Mabrouk , Dbeibia Amal , Mahdhi Abdelkarim , Romdhane Anis , Lawson Ata Martin , Daïch Adam , Harrath Abdel Halim , Jannet Hichem Ben , Othman And Mohamed 
Published: May 2023

As part of valorisation of agricultural waste into bioactive compounds, a series of structurally novel oleanolic acid ((3β-hydroxyolean-12-en-28-oic acid, OA-1)-phtalimidines (isoindolinones) conjugates 18a-v bearing 1,2,3-triazole moieties were designed and synthesized by treating a previously azide 4 prepared from OA-1 isolated from olive pomace (Olea europaea L.) with a wide range of propargylated phtalimidines using the Cu(I)-catalyzed click chemistry approach. OA-1 and its newly prepared analogues 18a-v were screened in vitro for their antibacterial activity against two Gram-positive bacteria, Staphylococcus aureus and Listeria monocytogenes; and two Gram-negative bacteria, Salmonella thyphimurium and Pseudomonas aeruginosa. Attractive results were obtained notably against L. monocytogenes. Compounds 18d, 18g and 18h exhibited the highest antibacterial activity when compared with OA-1 and other compounds in the series against tested pathogenic bacterial strains. Molecular docking study was performed to explore the binding mode of the most active derivatives against the target protein from L. monocytogenes. Results showed the importance of both hydrogen bonding and hydrophobic interactions with the target protein and are in favor to the experimental data.
Modeling Transient Flow Behavior of Off-Center Fractured Well with Multiple Fractures in Radial Composite Gas Reservoirs
Authors: Xu Youjie , Zuping Xiang , Yu Mengnan 
Published: May 2023

Summary
Vertical hydraulic fracturing is widely used to develop low-permeability gas reservoirs. Uneven distribution of formation permeability and stress leads to multiple-wing hydraulic fractures with different lengths, which results in the wellbore not being the center of the circular stimulated reservoir volume (SRV) region. Therefore, to simulate the wellbore pressure of this phenomenon, a semianalytical model of the off-center multiwing fractured well in radial composite gas reservoirs is presented and the corresponding solution method is shown. The model is verified with the numerical solution, and eight flow regimes can be distinguished under the ideal case, which includes bilinear flow, fracture interference, linear flow, radial flow of inner region, transition flow of inner region, and radial flow of inner region. Compared with the previous model in which the well is at the center of radial composite gas reservoirs, in this paper we present an obvious “step” after the inner region radial flow regime, which is related to the off-center distance and radius of the inner region. In addition, the effects of some important parameters (such as off-center distance, permeability mobility, inner region radius, and fracture distribution) on typical curves are discussed. Finally, field well testing data are used to verify the accuracy of the model.
scEC&amp;T-seq: a method for parallel sequencing of extrachromosomal circular DNAs and transcriptomes in single human cells
Authors: González Rocío Chamorro , Conrad Thomas , Kasack Katharina , Henssen Anton G. 
Published: May 2023

Abstract
Extrachromosomal DNA amplifications are common in cancer and are associated with decreased patient survival. A key feature of extrachromosomal circular DNA is its ability to be randomly mis-segregated to daughter cells promoting rapid intercellular heterogeneity. Understanding how extrachromosomal circular DNA dynamics contribute to intercellular heterogeneity remains crucial to better understand its role in tumor evolution and adaptation to therapy. Here, we introduce scEC&T-seq (single cell extrachromosomal circular DNA and transcriptomic sequencing), a method for parallel detection of extrachromosomal circular DNAs and full-length mRNA in single cancer cells. In this protocol, a single cell’s DNA is separated from its polyadenylated RNA as described by Macaulay et al. (2015)1. This is followed by removal of linear DNA through exonuclease digestion and further enrichment of circular DNA by rolling circle amplification with φ29 polymerase2-4. The separated mRNA from the same cell is processed using on-bead Smart-seq21. The duration of the entire procedure from cell sorting to library preparation is approximately 8 days. Our scEC&T-seq protocol has been validated in single cancer cells from neuroblastoma cell lines and primary tumors, and in normal single T-cells isolated from patient’s blood. Besides identifying large, oncogene-containing circular DNAs in cancer cells, our method also captures other smaller circular DNAs, which have been previously described in both cancer and non-malignant cells5. We envision that our method may enable the analysis of yet unknown prerequisites for the maintenance of both small and large circular DNA in cancers, but also in the context of other diseases and normal cellular development.
Age-specific variation of body mass and lipid reserves in Periplaneta americana (Blattodea: Blattidae)
Authors: Borah Nirmali , Hazarika Lakshmi 
Published: May 2023

Abstract

An experiment was conducted on age- specific variation of body mass (BM) and lipid reserves (LR) in Periplaneta americana (L.) (Blattodea: Blattidae). The BM and LR of adult females and males of P. americana fluctuated with age and the females were heavier and loaded with more lipids than the males. The BM and LR of both female and male P. americana showed positive correlation. In an another study on relationship between adult female BM and fecundity we found that an adult female fails to produce an ootheca or does not become reproductively active, until she attains a BM of 1.27 to 1.40 g; as such, 1.27 g may be considered the ‘threshold body mass’ below which a female fails to produce an ootheca. Moreover, positive and highly significant correlations between the female BM and ootheca number (ON) (r = 0.743) and BM and ootheca mass (OM) (r = 0.747) supported the idea that females with BM above the ‘threshold’ produce healthier oothecae with heavier offspring.
Responses of growth, yield and fruit quality of strawberry to elevated CO2, LED supplemental light, and their combination in autumn through spring greenhouse production.
Authors: Qiu Jiangbo , Cai Chuang , Shen Min , Gu Xinyue , Zheng Lei , Sun Lixia , Teng Yue , Yu Hongyan , Zou Luyi 
Published: May 2023

Abstract

During the whole growth cycle growth, yield and fruit quality of strawberry are often strongly affected by insufficient CO2 and low light in greenhouse production. However, it is still not clear what extent growth, yield and fruit quality of strawberry can be improved by elevated CO2 and supplementary light combination. We measured growth, yield and fruit quality of strawberries under four combinations of two levels of CO2, and two levels of light. Our results showed that yield enhancement throughout the growing season was 23.4% by elevated CO2, 21.46% by LED supplemental light, and 51.3% by their combination. Both elevated CO2 and LED supplemental light significantly increased soluble sugar content, but significantly decreased titratable acidity. LED supplemental light could partly or fully compensate for the negative impacts of elevated CO2 on soluble protein content, total phenol content, total flavonoid content, anthocyanin content, and total antioxidant capacity. Yield under four CO2 and light treatments was positively correlated with soluble sugar content, but negatively correlated with titratable acidity. Taken together, the combination of elevated CO2 and LED supplemental light largely improved both fruit yield and sweetness of strawberry during the autumn through spring in greenhouse. Optimal both CO2 and light is a worthwhile practice for improving strawberry production.
eDNA metabarcoding warms up a hotspot of marine biodiversity: Revealing underrepresented taxa in visual surveys and historical records from the Gulf of California
Authors: Loughlin Camila Mac , Valdivia-Carrillo Tania , Valenzuela-Quiñonez Fausto , Reyes-Bonilla Hector , Brusca Richard C , Munguia-Vega Adrian 
Published: May 2023

Abstract
Environmental DNA (eDNA) metabarcoding is revolutionizing biodiversity monitoring, but comparisons against traditional data are uncommon. We targeted eukaryotes through the 18S barcode amplified from water samples at 20 sites from the Gulf of California (GC), and contrasted eDNA against 316 simultaneous visual surveys and a historical database with over 5k species. From 61k Amplified Sequence Variants, we identified 850 eukaryotic families, of which half represent new compiled records, including 174 families of planktonic, benthic, and parasitic invertebrates. eDNA revealed many overseen taxa, highlighting higher taxonomic ranks within micro invertebrates, microscopic fungi, and other micro eukaryotes from the supergroups Stramenopiles, Alveolata, and Rhizaria. The database combining all methods has doubled the number of distinct phyla, classes and orders compared to the historical baseline, indicating biodiversity levels in the GC are much higher than previously assumed. The estimated proportion of historical taxa included in public reference databases was only 18% for species, explaining the small portion of eDNA reads that were taxonomically assigned to species level (13%). Each method showed different taxonomic biases, with eDNA missing vertebrates, visual surveys targeting only seven phyla, and the historical records focusing on macroinvertebrates, fish and algae. Although all methods recovered the main known biogeographic regionalization, eDNA data did not support the historical pattern of higher diversity in the Central than Northern GC. While combining methods provides a novel view of biodiversity that is much more comprehensive than any individual approach, our study highlights many challenges in synthesizing biodiversity data from traditional and novel sources.
Mass Fabrication and Smart Deployment of “Calibration-free" Miniature Solid-State Potentiometric Sensors towards Digital Water Infrastructure
Authors: Fan Yingzheng , Dai Zheqin , Xu Zhiheng , Qian Xin , Xiang Wenjun , Lounder Samuel , McNamara Cole , McCutcheon Jeffrey , Asatekin Ayse , Li Baikun 
Published: May 2023

Abstract
Achieving digital water infrastructures requires efficient in situ collection and swift interpretation of vast amounts of data under spatial and temporal variations. Existing water sensors suffer from inconsistent quality, incur frequent (re)calibration before and after deployment, and impede reliable data interpretation across water infrastructures. In this study, a novel quality control/quality assurance (QC/QA) regime was developed for mass fabrication of miniature solid-state potentiometric ion-selective membrane (MSP-ISM) sensors through material-wise and device-wise advancements. Our goal was to minimize the discrepancy of sensor readings, assure the consistence of sensor Nernst slope (NS) and standard potential (Eo), and ultimately alleviate the needs of (re)calibration towards calibration-free (CF). Specifically, the material-wise advancement was performed by modifying the key components (e.g., ion selective membrane and solid contact) of the polymer matrix to enhance the NS consistence among multiple pieces of NH4+ MSP-ISM sensors and reduce the standard deviation (SD) from 2.38 mV/dec to 0.27 mV/dec. The devise-wise advancement was conducted by fabricate polymer membranes using electrospray to and fabricating the substratum electrode using aerosol jet printing, through which the variation of the E0 values was diminished from 7.58 mV to 1.39 mV and the uniformity and homogeneity of each layer of sensors were improved. Furthermore, the recalibration-free capability of CF-MSP-ISM sensors was examined in real wastewater over 14 days consecutively, exhibiting excellent accuracy with a discrepancy of less than 2 mg/L against the lab-based validation results. Finally, smart deployment of multiple pieces of CF-MSP-ISM sensors along the length of an anoxic/oxic (A/O) system and precision feedback control were simulated a plug-flow aerobic flexible control (PFAFC) model. The simulation results demonstrate that energy consumption saving and greenhouse gas (GHG, with N2O as the example) emission reduction can achieve 38.4% and 68.7%, respectively, compared with the one using traditional “single-point” monitoring. This high-resolution sensor profiling-based precise control strategy has a great potential to renovate the existing treatment facilities highly relying on excess energy consumption to meet the effluent requirement and explore an energy-saving and resilient operation with mitigated GHG emission.
Using ScRNA-seq to Reveal Lymphocyte Responses to ALV-J in Bone Marrow Microenvironment
Authors: Wang Lecheng , Zhou Xinyi , Yang Gang , Xie Tao , Chen Shutao , Cui Zhifu , Liu Anfang , Wang Qigui , Wang Haiwei , Lan Xi 
Published: May 2023

Abstract
Background: The main tumors in chicken caused by avian leukemia virus (ALV) are leukemia. The bone marrow microenvironment is the genesis of leukemia, but little is known about the state of the chicken bone marrow microenvironment under avian leukemia virus subgroup J (ALV-J) infection. Meanwhile, alterations in the immune status of the bone marrow microenvironment are closely associated with the development of leukemia.
Results: In this article, scRNA-seq was used on chicken bone marrow lymphocytes with different states of ALV-J infection to identify marker genes, cell states, and subgroups of lymphocytes. A total of eighteen clusters and their potential marker genes were identified. Among them, eight T cell clusters, two B cell clusters, and five tumor-like cell clusters were identified, whereas three clusters could not be identified. Among ten lymphocyte clusters, double-positive T cells (cluster_2), B1-like B cells (cluster_7), and cytotoxic T cells (cluster_9) responded strongly to ALV-J infection. Their differentially expressed genes were highly enriched in immune-related pathways and viral infection-related pathways, and they accounted for a large proportion and variation in samples with different clinical symptoms of ALV-J infection. The immunosuppressive state of bone marrow microenvironment was stronger after the occurrence of more severe ALV-J infection. Regulatory T cells and CTLA4T cells were more predominant in samples with more severe ALV-J infection. Immunosuppressive factors TGFB1 and IL16 were expressed in multiple clusters, and the expression of TGFB1 and IL16 was higher in samples with more severe ALV-J infection. ALV-J infected all clusters, but in the same cluster of cells, a fraction of cells expressed ALV-J transcripts, whereas the other fraction did not. Meanwhile, in the same cluster of cells expressing ALV-J transcripts, the pathway associated with intracellular antiviral infection, “Signaling by Rho Family GTPases” was activated. By using IPA analysis software, some upstream regulatory elements (MYCand MCYN) responsible for this difference were predicted.
Conclusions: Decreased immunocompetence in the bone marrow microenvironment caused by ALV-J maybe associated with occurrence of leukemia. The cells in the same cluster showed different susceptibility to ALV-J. Our results could contribute to the understanding of bone marrow lymphocytes in different infection states of ALV-J.
Assessment of the Inter-observer Variability of the Radiomic Tools: BRODERS (Benign vs aggressive nODule Evaluation using Radiomic Stratification) and SILA (Score Indicative of Lung cancer Aggression) to Classify Lung Nodules
Authors: Al-Ghoula Faysal , Patel Khushbu , Falde Samuel , Rajagopalan Srinivasan , Maldonado Fabien , Peikert Tobias , Bartholmai Brian 
Published: May 2023

Abstract
Introduction: While CT lung cancer screening reduces lung cancer-specific mortality, there are remaining challenges. Radiomic tools promiss to address these challenges, however, they are subject to interobserver variability if semi-automated segmentation techniques are used. Herein we report interobserver variability for two validated radiomic tools, BRODERS (Benign versus aggRessive nODule Evaluation using Radiomic Stratification) and CANARY (Computer-Aided Nodule Assessment and Risk Yield). Methods We retrospectively analyzed the CT images of 95 malignant lung nodules of the adenocarcinoma spectrum using BRODERS and CANARY. Cases were identified at Mayo Clinic (n = 45) and Vanderbilt University Medical Center and Nashville/Veteran Administration Tennessee Valley Health Care System (n = 50). Three observers with different training levels (medical student, internal medicine resident and thoracic radiology fellow) each performed lung nodule segmentation. All methods were carried out in accordance with relevant guidelines and regulations Results Interclass correlation coefficients (ICC) of 0.77, 0.98 and 0.97 for the average nodule volume, BRODERS cancer probability and Score Indicative of Lesion Aggression (SILA) which summarizes the distribution of the CANARY exemplars indicated good to excellent reliability, respectively. The dice similarity coefficient was 0.79 and 0.81 for the data sets from the two institutions. Conclusion: BRODERS and CANARY are robust radiomics tools with excellent interobserver variability. These tools are simple and reliable regardless the observer/operator’s level of training.
HSP90 inhibition attenuated isoflurane-induced neurotoxicity in mouse and human neuroglioma cells
Authors: Zhang Chunlu , Chen Xi , Liu Ruizhu , Zhao Guoqing 
Published: May 2023

Abstract
Isoflurane is one of the most popular inhalation anesthetics in the clinical practice but presenting an increased risk of neuronal injury. Hsp90 has been indicated to present an important role for maintaining neuronal homeostasis under the stress. However, the effect of hsp90 during isoflurane exposure is poorly understood. Here in this study, we aimed to observe the protect effect of hsp90 inhibition, and investigate the potential regulatory mechanism after isoflurane exposure. We found that hsp90 inhibitor, 17AAG, showed a great protective effect in maintaining isoflurane-induced ferroptosis of mice hippocampus and cultured neuronal cells. We focus on the crucial protein GPX4 activity in ferroptosis, and found that the 17 AAG contributed its protect effect to preserve physiological level of GPX4 activity under isoflurane exposure, although 17AAG could restored the protein level of GPX4. Further, we observed CMA pathway was activated and then mediated GPX4 degradation under isoflurane exposure. And 17AAG could interfere the complex between hsp90 and lamp2a to inhibit CMA activity, followed by blocking GPX4 degradation, which further affected the isoflurane-induced ferroptosis. Based on these findings, we proposed that hsp90 inhibition as a protective mechanism against isoflurane-induced ferroptosis in neurons.
Design and Rationale for a Real-World Observational Cohort of Patients Living with Progressive Neurological Disease and their care partners: TARGET-NEURO Study
Authors: Fowler Nicole R. , Boustani Malaz A. , Meadows Eric S. , Morris Heather L. , Crawford Julie M. , Fried Michael W. 
Published: May 2023

AbstractBackgroundProgressive neurological diseases, such as Alzheimer’s disease and related dementias, Parkinson’s disease, and multiple sclerosis, are leading causes of disability and mortality. Chronic neurological diseases typically develop and progress over many years. Safety and effectiveness data, from long-term real-world sources are needed to guide therapeutic decision making and effectively measure pragmatic trials, and what matters most to patients and their care partners. The increasing availability of data from electronic health records is further enhanced by the growing sophistication of epidemiologic and statistical methods, such as causal inference.MethodsA longitudinal, observational study, TARGET-NEURO, will characterize the natural history of chronic neurological diseases in real world populations, observe practice patterns, directly evaluate patient and care partner experiences and understand the effectiveness and long-term safety of treatments. TARGET-NEURO is a cooperative consortium of centers treating patients with chronic neurological diseases, enrolling over one million participants across three cohorts: 1) Engaged, 2) Disease, and 3) Age. Selection for the Disease cohort is based upon diagnosis codes. Those who consent to completing patient-reported outcomes with optional consent for biospecimen collection, will be enrolled into the Engaged cohort. Care partners are also eligible to participate in surveys. The Age cohort includes adults at least 60 years old, regardless of diagnoses.ConclusionsTARGET-NEURO establishes a large, robust database from participants in both academic medical centers and community practice settings to address important clinical questions. The outcomes can support improved understanding of how different patients progress in their disease and how trajectories vary based on sociodemographic factors, co-morbidities and risk factors. The results can support novel prevention and screening strategies, earlier and more precise diagnoses, and appropriate and equitable use of therapies.Trial registrationClinicaltrials.gov identification number: NCT05796037