bluuebunny/crossref_metadata_2025_split huggingface dataset:
Diverse Type I and Type II methanotrophs cultivated from an Indian freshwater wetland habitat
Authors: Mohite Jyoti A. , Manvi Shubha S. , Pardhi Kajal , Bahulikar Rahul A. , Deshpande Shrinidhi , Patange Sanjana , Joshi Mansi , Kulkarni Sharvari , Rahalkar Monali C. 
Published: June 2023

Abstract
Wetlands are the largest natural sources of microbial methane and contribute to approximately 23% of the global methane. Such wetland patches serve as rich habitats for aerobic methanotrophs. Limited knowledge of methanotrophs from tropical wetlands widens the scope of study from these habitats. In the present study, a freshwater wetland from a tropical region in India was sampled serially diluted to obtain methanotrophs in culture. This was followed by isolation on agarose-containing plates, incubated under a methane: air atmosphere. Methanotrophs are difficult to cultivate, and a very few cultures of methanotrophs are available from tropical wetlands. In our present study, we could culture a diverse community of methanotrophs from six genera, namely, Methylomonas, Methylococcus, Methylomagnum, Methylocucumis (Type I methanotrophs), and Methylocystis, Methylosinus (Type II methanotrophs). A strain of the newly described methanotroph, Methylocucmis oryzae, for which only two strains have been documented worldwide, was also successfully cultured in the study. A high abundance of methanotrophs (106-1010 methanotrophs/g fresh weight) was observed in the samples. A Methylococcus strain representing a putative novel species was also isolated. A large number of Methylomonas strains were cultured, all related to Methylomonas koyamae. Our study is one of the first documentation of culturable methanotrophs from a tropical wetland and reports that such wetland patches may contain high counts and diversity of methanotrophs. The isolated methanotrophs can act as models for studying methanotroph-based methane mitigation from wetland habitats and can be used for various mitigation and valorization applications.
The effects of Kinesio taping on pain intensity and soft tissue mechanical properties in non- specific low back pain
Authors: Berger-Pasternak Bernadetta , Sipko Tomasz 
Published: June 2023

Abstract
Objective
To assess the effects of Kinesio Taping (KT), with or without tension, and post-isometric muscle relaxation (PIR) on pain intensity and the mechanical properties of myofascial tissues in chronic low back pain (LBP) patients.
Methods
Study participants (n = 64), aged 20 to 60, were randomly assigned to one of the three intervention groups. The numerical rating scale (NRS) assessed pain intensity at rest, and the MyotonPRO© measured tension, stiffness, and relaxation in the erector spinae on both sides of the lumbar spine. The examinations were undertaken before the intervention, post-interventions, and at a seven-day follow-up.
Results
Pain level was reduced for all groups (F (4, 116) = 39.2010, p < 0.01). In addition, post hoc analysis of the KT group showed significant increases in the tension and stiffness of the erector spinae and significantly decreased relaxation between the pre-intervention and follow-up measurements, but only on the non-dominant side (p < 0.01).
Conclusions
Assuming that the lack of asymmetry in muscle tension, stiffness, and relaxation is a normative value, it can be interpreted that the applied interventions were insufficient. As such, KT should be considered a complementary method of pain reduction in conjunction with conventional physiotherapy in LBP patients.
Study on the relationship of interface morphology and mechanical properties of explosive welded titanium/duplex stainless steel
Authors: Wang Minjing , Hu Jianian , Xie Quanming , Luo Ning , Li Xiaojie , Chen Xiang , Chen Zhen 
Published: June 2023

Abstract

This study investigated the interface morphology and mechanical properties of titanium (TP 270C)/duplex stainless steel (SUS 821L1) near the lower limit of the explosive welding window. Seven samples with different welding parameters near the lower limit of the welding window were evaluated; the interface morphology was analyzed by optical microscopy (OM), scanning electron microscopy (SEM), computed tomography (CT), and smoothed particle hydrodynamics (SPH) simulation. The jet, interface shape, and temperature at the interface were obtained by simulation, demonstrating consistent interface shapes in the experimental results and simulation results. The energy produced at the interface was proportional to the element diffusion area. To assess the differences in mechanical properties between each sample, tensile shear tests and 90 bending tests were conducted.
Pioneering Discoveries in Wireless Power Generation across Wide Geographical Ranges Harnessing Medium-Wave Radio Broadcasting Infrastructure
Authors: Shoji Eiichi 
Published: June 2023

Abstract
This study conducted field experiments using the radio-wave energy harvester of hoop-shaped radio (HOOPRA) to elucidate the relationship between the distance from the medium-wave (MW) radio broadcasting station and the resonance voltage as an index of harvested energy. Accordingly, we derived a numerical equation that described the relationship between the distance and voltage with a strong correlation coefficient of R2 > 0.996. HOOPRA can illuminate ultrabright white, green, and red LEDs within a 5 km radius of the public NHK JOFG (927 kHz, 5 kW) in Fukui, thereby serving as a valuable energy source for the 150,000 residents (60,000 households) in this region. The public JOAK (594 kHz, 300 kW) and JOAB (693 kHz, 500 kW) were examined for similar field experiments in the Kanto region. The results revealed that HOOPRA could illuminate ultrabright white, green, and red LEDs within a radius of 30 km and ultrabright red LEDs within a radius of 50 km from JOAB, which will benefit the 23 million residents (1.1 million households) in this region.
Antibacterial susceptibility patterns and factors associated with secondary peritonitis among patients with acute abdomen: a cross-sectional study
Authors: Godefroy Nyenke Bassara , Muhumuza Joshua , Molen Selamo Fabrice , Waziri Musa Abbas , Kagenderezo ByaMungu Pahari , Mauricio William , Wandabwa Joel , Francois Bisingurege Kagoro , Agwu Ezera , Okedi Xaviour Francis 
Published: June 2023

Abstract

Introduction: Secondary peritonitis is the second leading cause of sepsis worldwide. Drug-resistance to peritoneal cavity bacterial infection remains a public health threat especially in resource limited settings of Africa including Uganda. This study was aimed at determining the antibacterial susceptibility patterns and factors associated with secondary peritonitis among patients with acute abdomen who underwent surgery at a Regional Referral Hospital in Uganda. Methods: This was a cross sectional study conducted at Hoima Regional Referral Hospital (HRRH) that enrolled 126 patients with acute abdomen. After laparotomy, clinical samples were aseptically collected from patients with secondary peritonitis for culture and sensitivity using standard Microbiological methods. Binary logistic regression was used to identify factors associated with secondary peritonitis among patients with acute abdomen. Results: Majority of the patients were males (61.9%) with a mean age of 37.9(SD +/- 21.8). Secondary peritonitis was found in 57(45.2%) of the patients. Gram-negative bacteria were the most commonly isolated organisms with Escherichia coli (35.8%) and Klebsiella spp (17.0%) predominating. Imipenem 88.8%(8/9), Amikacin 88.8%(8/9), Ciprofloxacin 44.4%(4/9) and Gentamicin 44.4%(4/9) demonstrated sensitivity to the different isolated organism at varying degrees. Being a male (AOR = 3.658; 95% CI = 1.570–8.519, p = 0.003) and presenting 3 days after onset of symptoms (AOR = 2.957; 95%CI = 1.232–7.099, p = 0.015) were independently associated with secondary peritonitis. Conclusion Imipenem, Amikacin, Ciprofloxacin and Gentamicin should be considered for empirical therapy in cases of secondary peritonitis. Patients, more especially males with abdominal pain should be encouraged to present early to the hospital in order to minimise progression to secondary peritonitis.
Antimicrobial and phytotoxic secondary metabolites produced by Xylaria necrophora, an emerging pathogen of soybean, play key roles in infection biology
Authors: Solórzano José E. , Ganiu Moshood O. , Donnarumma Fabrizio , Garcia-Aroca Teddy , Kartika Rendy , Richards Jonathan K. , Houten Joshua P. Van , Gremillion Michelle R. , Price Paul P. , Doyle Vinson 
Published: June 2023

Abstract
Xylaria species are recognized globally given their common occurrence as wood-degrading saprophytes in forest ecosystems. They are known for their ability to produce secondary metabolites with diverse bioactivity. A few are pathogens, but Xylaria necrophora is the only species known to be a pathogen of an annual crop, causing taproot decline (TRD) on soybean [Glycine max (L) Merr.]. Recent work determined that culture filtrates produced by X. necrophora are phytotoxic and likely responsible for the foliar symptoms of the disease. We demonstrate that the foliar symptoms may be the result of root inhibition as culture filtrates also stop root development. Xylaria necrophora also produces antimicrobial secondary metabolites (SMs) that likely mediate interactions with other soil microbes to set the stage for plant infection. Bioassay-guided fractionation and extracted fractions from cell-free culture filtrates (CFs) led to the identification of SMs using LC-MS and LC-MS/MS analyses: 1. 18-Deoxy-19,20-epoxycytochalasin Q, 2. 19,20-epoxycytochalasin Q, 3. 5-(1-Hydroxybutyl)-6-(hydroxymethyl)-2H-pyran-2-one, 4. 6-[(1R)-1-Hydroxypentyl]-4-methoxy-2H-pyran-2-one, 5-6. Cytochalasin C and D, 7. Xylopimarane, 8. Hirsutatin A, 9. Xylaric acid C and 10. Zygosporin E. SMs 1-7 presented antimicrobial activity against fungi and 1, 2, 5, 6, 8, 9, and 10 were phytotoxic to soybean. SMs 1, 2, 5, and 6, were both phytotoxic and antimicrobial. This is the first report identifying SMs produced by X. necrophora. SMs capable of both causing phytotoxicity and inhibiting a diversity of fungal pathogens suggests an important role for these SMs in the etiology of TRD.
Determination of Plant Regeneration Protocol of “Viking” Aronia Cultivar in the In Vitro Conditions
Authors: NAS ZEYNEP , EŞİTKEN Ahmet , PIRLAK Lütfi 
Published: June 2023

Abstract

In this study,in vitromicropropagation of 'Viking' aronia cultivar was investigated. For this purpose, different cytokinin and doses were tested in MS food medium for propagation medium. Except for cytokinins, low doses of IBA and GA were added and equal doses were used in all applications. For the rooting medium, different doses of IBA (0.125, 0.25, 0.5 and 1.0 mg l-1) and activated charcoal (0.5 and 1.0 g l-1)were tested in MS and ½MS mediums. A mixture of perlite and peat was preferred during acclimatization. No plant death was encountered in the study, and 100% viability was detected in all applications. The highest number of shoots was detected in media containing TDZ; however, most of the shoots that can be used as micro cuttings were obtained from media containing BA and mT. The highest number of shoots occurred at 1.0 mg l-1doses of media containing BA and mT, and 16.83 and 21.76 shoots/explants were obtained, respectively. 100% rooting was achieved in all applications, including the control. The highest root number was detected in ½MS medium (10.17 roots/explant) containing 1.0 mg l-1IBA. Higher root number was obtained in ½MS medium compared to MS medium; However, it was determined that the root length decreased with the increase in the number of roots. The acclimatization phase has resulted in high success, 98% of plants in ½MS medium and 92.4% of plants in MS medium survived.
Genomic expression of MSR1 in solid tumors associates with response to anti-PD1 and anti-CTLA4 therapies
Authors: Sanvicente Adrián , Díaz-Tejeiro Cristina , Nieto-Jiménez Cristina , Paniagua-Herranz Lucia , Cade Igor López , Balázs Győrffy , Moreno Victor , Pérez-Segura Pedro , Calvo Emiliano , Ocana Alberto 
Published: June 2023

Abstract
Immuno-oncology has gained momentum with the approval of antibodies that have demonstrated clinical activity in different indications. Unfortunately, for anti PD (L)1 agents in monotherapyonly half of the treated population will achieve a clinical response. For other agents like anti-CTLA4 antibodies, no biomarker exists, and tolerability can limit the administration of this compound. In this study, using publicly available genomic datasets, we evaluated the expression of the macrophage scavenger receptor-A (SR-A) (MSR1) and its association with response to check point inhibitors (CPI). MSR1 was mainly and differently expressed in GBM, PAAD, ESCA, STAD, SKCM, OV, KIRC, THYM, HNSC and BRCA, within the tumor microenvironment. MSR1 was associated with the presence of macrophages, dendritic cells (DCs) and neutrophils in most of the studied indications. The presence of MSR1 was associated with macrophages with a pro-tumoral or M2 phenotype and correlated with TIM3 expression. MSR1 predicted favorable overall survival in patients treated with anti-PD1 (HR: 0.56, FDR: 1%, p = 2.6e-05); anti PD-L1 (HR: 0.66, FDR:20%, p = 0.00098) and anti-CTLA4 (HR: 0.37, FDR:1%, p = 4.8e-05). When studying specifically skin cutaneous melanoma (SKCM), we observed a similar effect: anti-PD1 (HR: 0.65, FDR: 50%, p = 0.0072); and anti-CTLA4 (HR: 0.35, FDR:1%, p = 4.1e-05). In a different dataset of SKCM patients, expression of MSR1 predicted clinical response to anti-CTLA4 (AUC: 0.61, p = 2.9e-02). In conclusion, we described the expression of MSR1 in some solid tumors and its association with innate cells and M2 phenotype macrophages. Of note, MSR1 presence predicted response to CPI particularly anti-CTLA4 therapies in different cohorts of patients. Future studies should prospectively explore the association of MSR1 expression and response to anti-CTLA4 strategies in solid tumors.
Epidemic propagation dynamics of an SIS model on networks with population mobility between regions
Authors: Zhang Hanqi , Sun Zhongkui , Liu Yuanyuan 
Published: June 2023

Abstract
The impact of population mobility is non-negligible on the spread of infectious diseases. In this study, we construct a network SIS model that accounts for the impact of population mobility between regions, using a nonlinear infection rate and dividing the infected into symptomatic and asymptomatic groups. First, the study analyses the stability of equilibrium points by constructing the Lyapunov function and validating it using numerical experiments. Secondly, a special form of the basic regeneration number was attained using the next generation matrix method. This result inspires us to pay more attention to asymptomatic infections in the disease control process so that we can stabilize the population to a state where asymptomatic infections are extinct as quickly as possible and facilitate the subsequent epidemic control. Additionally, the results of numerical simulations further demonstrate that for people with different degrees, we can adopt corresponding ideas respectively to determine the optimal movement rate, which will avoid disease infection to a greater extent. Our study will serve as a guide for the development of infectious disease prevention and control policies.
An innovative model for predicting coronary heart disease using TyG-index: A machine learning-based cohort study
Authors: Mirjalili Seyed Reza , Soltani Sepideh , HeidaryMeibodi Zahra , Marques-Vidal Pedro , Kraemer Alexander , Sarebanhassanabadi Mohammadtaghi 
Published: June 2023

Abstract

Background Various coronary heart disease (CHD) predictive models have been developed for predicting CHD incidence, but none of them has optimal predictive value. Although these models consider diabetes as an important CHD risk factor, they did not consider insulin resistance or Triglyceride. Methods Two-thousand participants of a community-based Iranian population, aged 20–74 years, were investigated with a mean follow-up of 9.9 years (range: 7.6 to 12.2). The association between TyG-index (a logarithmised combination of fasting blood glucose and triglyceride that demonstrates insulin resistance) and CHD was investigated using multivariate Cox proportional hazard models. Diabetes was substituted for TyG-index in CHD prediction models developed using machine learning. CHD-predicting TyG-index cut-off points were calculated. Results The incidence of CHD was 14.5%.As compared to the lowest quartile of TyG-index, the fourth quartile had a fully adjusted hazard ratio of 2.32 (CI: 1.16–4.68, p-trend 0.04). In order to predict coronary heart disease, TyG-index > 8.42 had the highest negative predictive value. Machine learning models that predicted CHD based on TyG-index performed significantly better than those based on diabetes. TyG-index was not only more important than diabetes in prediction of CHD; it was the most important factor in machine learning models. Conclusion TyG-index can be used in clinical practice and predictive models as a highly valuable index for predicting and preventing CHD.
The pattern and prognostic factors for kidney function progression in children with acute kidney injury and acute kidney disease
Authors: Hui Wun Fung , Chan Vivian Pui Ying , Cheung Wing Lum , KU Shu Wing , Hon Kam Lun 
Published: June 2023

Abstract
Background: There is a paucity of data describing the progression of kidney function from acute kidney injury (AKI) to acute kidney disease (AKD) among children.
Methods: A prospective cohort study on pediatric AKI was conducted from 6/2020 to 6/2021 on critically ill children aged 1 month to ≤18 years old. The 90-day natural history of kidney function progression was described. Prognostic factors associated with development of AKD and various outcomes were identified.
Results: Among the 253 episodes of admission, 58.1% were male with a median (interquartile range) age of 4.9 (9.7) years The AKI incidence was 41.9% and 73.6% of children with AKI developed AKD. Parameters signifying more severe and prolonged AKI and higher degree of nephrotoxic medication exposure were significant determinants of AKI progression and AKD development. At 90 days, 31.5% of children had serum creatinine level (SCr) ≥1.5 times of baseline, 10.4% had an estimated glomerular filtration rate (eGFR) <90ml/min/1.73m2 and the mortality was 6.7%. Children with AKI followed by AKD had the highest risk of having SCr ≥1.5 times of baseline (HR 13.59 [6.07, 30.46]) and eGFR <90ml/min/1.73m2 (HR 12.56 [3.19, 49.38]). Persistent AKI was significantly associated with 90-day mortality (HR 7.06 [2.72, 18.3]).
Conclusions: Pediatric AKI survivors were at risk of developing AKD. The severity and duration of AKI and AKD were important determinants of outcomes. Children with both AKI and AKD carried the highest risk of kidney function non-recovery at 90 days. A follow-up visit is warranted for children who survived AKI.
Pulsed-aerosol assisted low-pressure plasma for thin film deposition
Authors: Carnide Guillaume , Simonnet Claire , Sadek Thibault , Parmar Divyesh , Zavvou Zaccharoula , Girardeau Adèle , Pozsgay Vincent , Verdier Thomas , Villeneuve Christina , Kahn Myrtil Louise , Stafford Luc , Clergereaux Richard 
Published: June 2023

Abstract

Plasma-enhanced chemical vapor deposition (PE-CVD) is intensively studied and developed to form (multi-)functional thin films. Generally produced in gases or vapors of thermodynamically stable and chemically inert precursors, aerosol assisted plasma process becomes an alternative as it enables to inject various liquid solutions independently of these characteristics. This study examines the case of pentane aerosols injected in pulsed mode in a low-pressure RF plasma. It produces diamond-like carbon thin films with material balance larger than those obtained in gaseous processes. Here, the deposition process is controlled by the pulsed injection. Indeed, the dynamics of thin film deposition result in time-dependent mechanisms at the pulse scale related to the temporary increase of the working pressure and the presence of liquid droplets in the plasma volume. Hence, thin film deposition is controlled by plasma-surface as well as plasma-droplets interactions. Consequently, aerosol-assisted plasma processes are really relevant for the deposition of (multi-)functional coatings.
Underwater versus conventional endoscopic mucosal resection for colorectal lesions: a systematic review and meta-analysis of randomized clinical trials
Authors: Souza Matheus Henrique Gonçalves , Santo Paula Arruda do Espirito , Maluf-Filho Fauze , Lenz Luciano 
Published: June 2023

Abstract

Purpose: Conventional endoscopic mucosal resection (CEMR) is the established method for the resection of non-pedunculated colorectal lesions (NPCRL) ≥ 10mm. In the last decade, underwater endoscopic mucosal resection (UEMR) has been introduced as a potential alternative. The aim of this systematic review with meta-analysis is to compare the recurrence and safety of UEMR and CEMR by analyzing only randomized controlled trials (RCTs).
Methods: We systematically searched PubMed, Cochrane Library and EMBASE until April 2023. Studies met the following inclusion criteria: (1) RCTs, (2) comparing UEMR with CEMR, (3) NPCRL ≥ 10 mm, and (4) reporting the outcomes of interest. Primary outcomes were recurrence and safety. Secondary outcomes were en bloc, R0, complete resection, clipping and adverse events per type.
Results: Five RCTs were included. UEMR was associated with a lower recurrence rate (OR: 0.56; 95% CI: 0.32-0.97). Thus, the RR of recurrence was 1.7 times higher in the CEMR group (95% CI, 1.04-2.77). There was no significant difference in the pooled safety analysis. UEMR showed better en bloc resection rates (OR: 1.54; 95% CI: 1.15-2.07), but subgroup analysis showed comparable rates in lesions ≥20 mm. R0 resection was higher in UEMR (OR: 1.72; 95% CI: 1.23-2.41). Other outcomes were not different between the 2 groups.
Conclusions: UEMR is as safe as CEMR, with a higher overall R0 rate and a higher en bloc resection rate for lesions < 20 mm, leading to a lower overall recurrence rate. The results of this meta-analysis support the widespread use of UEMR.
Epidemiologic Investigation of Eye Symptoms Related to COVID-19 among Outpatient Population
Authors: Eslami Fatemeh , Khazaei Salman , Ghayour Anahita Eslami- , Mohammadi Tahere 
Published: June 2023

Abstract
Since 2019, with the spread of the coronavirus and the emergence of new strains around the world, the effects of this virus on various organs of the body have been identified through various studies. According to these studies, the coronavirus has the ability to be transmitted through mucosal membranes, including respiratory membranes such as the nasal and conjunctival membranes. As the eyes are connected to the nasal duct through the inferior meatus, it is possible for the virus to be transmitted through this route. Wearing a mask can reduce the chance of transmission, but it can also lead to eye symptoms such as dryness. Additionally, individuals with COVID-19 experience various symptoms in their body systems. Some of these symptoms can affect the visual system and lead to blurry vision, dry eyes, foreign body sensation, tearing, and more. These symptoms can cause early eye fatigue and reduce the quality of academic and occupational performance. Since there is limited research in this area and considering the importance of this disease and its consequences, we decided to conduct an observational study on a number of COVID-19 patients referred to the clinic.