bluuebunny/crossref_metadata_2025_split huggingface dataset:
Genomic exploration of coral-associated bacteria: identifying probiotic candidates to increase coral bleaching resilience in Galaxea fascicularis
Authors: Doering Talisa , Tandon Kshitij , Topa Sanjida H. , Pidot Sacha J. , Blackall Linda L. , van Oppen Madeleine J. H. 
Published: August 2023

Abstract
Background
Reef-building corals are acutely threatened by ocean warming, calling for active interventions to reduce coral bleaching and mortality. Corals associate with a wide diversity of bacteria which can influence coral health, but knowledge of specific functions that may be beneficial for corals under thermal stress is scant. Under the oxidative stress theory of coral bleaching, bacteria that scavenge reactive oxygen (ROS) or nitrogen species (RNS) are expected to enhance coral thermal resilience. Further, bacterial carbon export might substitute the carbon supply from algal photosymbionts, enhance thermal resilience and facilitate bleaching recovery. To identify probiotic bacterial candidates, we sequenced the genomes of 82 pure-cultured bacteria that were isolated from the emerging coral model Galaxea fascicularis.

Results
Genomic analyses showed bacterial isolates were affiliated with 37 genera. Isolates such as Ruegeria, Muricauda and Roseovarius were found to encode genes for the synthesis of the antioxidants mannitol, glutathione, dimethylsulfide, dimethylsulfoniopropionate, zeaxanthin and/or β-carotene. Genes involved in RNS-scavenging were found in many G. fascicularis-associated bacteria, which represents a novel finding for several genera (including Pseudophaeobacter). Transporters that are suggested to export carbon (semiSWEET) were detected in seven isolates, including Pseudovibrio and Roseibium. Further, a range of bacterial strains, including strains of Roseibium and Roseovarius, revealed genomic features that may enhance colonisation and association of bacteria with the coral host, such as secretion systems and eukaryote-like repeat proteins.

Conclusions
Our work provides an in-depth genomic analysis of the functional potential of G. fascicularis-associated bacteria and identifies novel combinations of traits that may enhance the coral’s ability to withstand coral bleaching. Identifying and characterising bacteria that are beneficial for corals is critical for the development of effective probiotics that boost coral climate resilience.
BinaRena: a dedicated interactive platform for human-guided exploration and binning of metagenomes
Authors: Pavia Michael J. , Chede Abhinav , Wu Zijun , Cadillo-Quiroz Hinsby , Zhu Qiyun 
Published: August 2023

Abstract
Background
Exploring metagenomic contigs and “binning” them into metagenome-assembled genomes (MAGs) are essential for the delineation of functional and evolutionary guilds within microbial communities. Despite the advances in automated binning algorithms, their capabilities in recovering MAGs with accuracy and biological relevance are so far limited. Researchers often find that human involvement is necessary to achieve representative binning results. This manual process however is expertise demanding and labor intensive, and it deserves to be supported by software infrastructure.

Results
We present BinaRena, a comprehensive and versatile graphic interface dedicated to aiding human operators to explore metagenome assemblies via customizable visualization and to associate contigs with bins. Contigs are rendered as an interactive scatter plot based on various data types, including sequence metrics, coverage profiles, taxonomic assignments, and functional annotations. Various contig-level operations are permitted, such as selection, masking, highlighting, focusing, and searching. Binning plans can be conveniently edited, inspected, and compared visually or using metrics including silhouette coefficient and adjusted Rand index. Completeness and contamination of user-selected contigs can be calculated in real time.
In demonstration of BinaRena’s usability, we show that it facilitated biological pattern discovery, hypothesis generation, and bin refinement in a complex tropical peatland metagenome. It enabled isolation of pathogenic genomes within closely related populations from the gut microbiota of diarrheal human subjects. It significantly improved overall binning quality after curating results of automated binners using a simulated marine dataset.

Conclusions
BinaRena is an installation-free, dependency-free, client-end web application that operates directly in any modern web browser, facilitating ease of deployment and accessibility for researchers of all skill levels. The program is hosted at https://github.com/qiyunlab/binarena, together with documentation, tutorials, example data, and a live demo. It effectively supports human researchers in intuitive interpretation and fine tuning of metagenomic data.
Psychosocial factors and chronic spontaneous urticaria: a systematic review
Authors: Donnelly Jennifer , Ridge Katie , O’Donovan Roisin , Conlon Niall , Dunne Pádraic J. 
Published: August 2023

Abstract
Background
Psychosocial factors have been informally associated with Chronic Spontaneous Urticaria (CSU); however, the relationship between psychosocial factors and CSU remains relatively unexplored in the scientific literature.

Objective
This review aims to provide an evaluation of peer reviewed studies exploring psychosocial factors and CSU.

Methods
A systematic search was performed over four databases identifying studies exploring psychosocial factors in relation to CSU published between the years 1995 and 2022.

Results
Eighteen studies were included for narrative analysis, and 33 psychosocial factors were identified. These were split into two subgroups: psychosocial factors that were associated with CSU symptoms aggravation/onset (n = 20), and psychosocial factors expected to be impacted by CSU symptoms (n = 13).

Conclusion
This review has highlighted a need for more research and interventions to support individuals with psychosocial factors involved in CSU.
Sialendoscopy approach in treating juvenile recurrent parotitis: A systematic review
Authors: Soriano-Martín D. , García-Consuegra L. , Junquera L. , Rodríguez-Santamarta T. , Olay S. , Junquera-Olay S. 
Published: August 2023

Background Juvenile recurrent parotitis (JRP) is characterized by recurrent episodes of painful parotid swelling in children. The purpose of this systematic review was to determine the diagnostic and therapeutic effectiveness of sialendoscopy in children affected by JRP. Methods A systematic literature search was performed in PubMed, EMBASE, Scopus and the Cochrane Library until April 2022, without language restrictions or specified start date. Quality assessment was performed using the Newcastle–Ottawa Scale (NOS). Results Our review included 524 patients and 646 sialendoscopies. The sample sizes of the different studies ranged from 3 to 77 subjects. Most authors performed sialendoscopy under general anesthesia. The mean percentage of recurrences observed was 25.1% (95% confidence intervals) (CI 23.6–26.6). There was a statistically significant relationship between the number of attacks/year and recurrences ( p < 0.05). The percentage of recurrences according to the type of irrigation/flushing used ranged from 22.2% to 25.2%, with no significant differences between the use of corticosteroids alone (25.2% of recurrences), corticosteroids plus antibiotics (25% of recurrences) or saline alone (22.2% of recurrences). Sialoendoscopy has proved in all cases to be a valid method for the diagnosis of JRP, but it does not allow a reliable differential diagnosis with other autoimmune parotitis such as Sjögren's syndrome. Conclusion According to our results, parotid sialoendoscopy was 74.9% effective as a primary treatment in the prevention of recurrent symptoms in JRP. The type of ductal irrigation used did not significantly influence the prognostic outcome.
Caregiver Burden and Its Relationship to Health-Related Quality of Life in Craniopharyngioma Survivors
Authors: Kayadjanian Nathalie , Hsu Eugenie A , Wood Amy M , Carson Dean S 
Published: August 2023

Abstract

Context
Craniopharyngioma (CP) is a rare brain tumor associated with severe comorbidities that reduce survivor health-related quality of life (HRQOL). However, CP impact on caregivers is unknown.

Objective
To measure caregiver burden and examine its relationship to survivor HRQOL and other determinants in CP.

Methods
Eighty-two participants who self-identified as caregivers of CP survivors responded to an online survey including caregiver-reported Pediatric Quality of Life Inventory (PedsQL), and the Zarit Burden Interview (ZBI).

Results
Caregivers reported an average of 13 out of 29 health conditions in survivors following tumor treatment, including excess weight, hypopituitarism, fatigue, mood, cognition, social issues, temperature dysregulation, visual impairment, and sleep problems. Strikingly, 70% of survivors who experienced obesity also experienced hyperphagia. ZBI scores were moderate with a median of 37. PedsQL total scores were poor with a median of 46.2. ZBI scores were independent of caregiver level of education and care duration. Both scores were independent of income, survivor age, gender, age at diagnosis, or tumor recurrence. In contrast, both scores depended on the number (P &lt; .001) and the type of survivor health problems, with significantly worse scores for caregivers or survivors with symptoms of hypothalamic dysfunction (P &lt; .001) including hyperphagia but not obesity. PedsQL total scores significantly predicted ZBI scores (P &lt; .001).

Conclusion
Survivor poly-symptomatology predicted and incurred significant caregiver burden. Our study separated hyperphagia and obesity and identified hyperphagia and other hypothalamic dysfunction symptoms as understudied issues. Altogether, these findings draw particular attention to the unmet needs of CP survivors and their caregivers.
Risk Factors, Management, and Outcome of Gastric Venous Congestion After Total Pancreatectomy: An Underestimated Complication Requiring Standardized Identification, Grading, and Management
Authors: Stoop Thomas F. , von Gohren André , Engstrand Jennie , Sparrelid Ernesto , Gilg Stefan , Del Chiaro Marco , Ghorbani Poya 
Published: August 2023

Abstract
Background
Gastric venous congestion (GVC) after total pancreatectomy (TP) is rarely studied despite its high 5% to 28% incidence and possible association with mortality. This study aimed to provide insight about incidence, risk factors, management, and outcome of GVC after TP.

Methods
This retrospective observational single-center study included all patients undergoing elective TP from 2008 to 2021. The exclusion criteria ruled out a history of gastric resection, concomitant (sub)total gastrectomy for oncologic indication(s) or celiac axis resection, and postoperative (sub)total gastrectomy for indication(s) other than GVC.

Results
The study enrolled 268 patients. The in-hospital major morbidity (Clavien-Dindo grade ≥IIIa) rate was 28%, and the 90-day mortality rate was 3%. GVC was identified in 21% of patients, particularly occurring during index surgery (93%). Intraoperative GVC was managed with (sub)total gastrectomy for 55% of the patients. The major morbidity rate was higher for the patients with GVC (44% vs 24%; p = 0.003), whereas the 90-day mortality did not differ significantly (5% vs 3%; p = 0.406). The predictors for major morbidity were intraoperative GVC (odds ratio [OR], 2.207; 95% confidence interval [CI], 1.142–4.268) and high TP volume (> 20 TPs/year: OR, 0.360; 95% CI, 0.175–0.738). The predictors for GVC were portomesenteric venous resection (PVR) (OR, 2.103; 95% CI, 1.034–4.278) and left coronary vein ligation (OR, 11.858; 95% CI, 5.772–24.362).

Conclusions
After TP, GVC is rather common (in 1 of 5 patients). GVC during index surgery is predictive for major morbidity, although not translating into higher mortality. Left coronary vein ligation and PVR are predictive for GVC, requiring vigilance during and after surgery, although gastric resection is not always necessary. More evidence on prevention, identification, classification, and management of GVC is needed.
Available therapies for the treatment of obesity. Are lifestyle modifications and pharmacotherapy enough to reduce weight? - review of the literature
Authors: Pierzak-Stępień Monika 
Published: August 2023

Introduction: The World Health Organization (WHO) defines obesity as "abnormal or excessive accumulation of adipose tissue that poses a health risk". It results from the interdependencies between genetic, cultural, and socio-economic factors. The choice of therapy in the treatment of overweight and obesity depends on body mass index (BMI), individual predispositions, comorbidities and weight history. Among the treatment methods stand out, dietary modification, lifestyle changes, pharmacotherapy, and bariatric surgery. Each of these treatments can be used individually or as a combination treatment using knowledge of physiology.
The aim of the study is to assess the effectiveness of overweight and obesity treatment using pharmacotherapy and lifestyle modification.
Material and Methods: The research material was collected by searching databases with scientific articles about obesity, pharmacotherapy in obesity, the impact of lifestyle modifications, behavioral behavior, and physical activity on the reduction of body fat. The scientific database Pubmed and Web of Science were used. Research articles, systematic reviews and meta-analyses were included.
Summary Obesity is an epidemic of the 21st century, bringing with it several negative consequences. Prevention of the disease, quick diagnosis, and implementation of appropriate treatment to reduce unnecessary fat and excess weight are extremely important. Lifestyle change including dietary modification, physical activity and pharmacotherapy are promising treatments for overweight and obesity.
A pill full of sunshine. Vitamin D supplementation among polish students
Authors: Gaik Magdalena , Gazda Dawid , Bogacz Rafał , Uram Ewa , Woźniak Justyna , Womperski Karol , Magda Inga , Osuch Magdalena 
Published: August 2023

Introduction and purpose of work:
Vitamin D has a significant impact on the functioning of the human body. Vitamin D deficiency is a public health problem worldwide in all age groups including Poland. Supplementation is recommended in the general population with the use of cholecalciferol and should be individualized depending on age, body weight, the sun exposure, diet, and lifestyle.
The aim of study was to analyze vitamin D supplementation habits among polish students in two divided groups: medical students and non-medical students.
Methods:
The tool used to carry out the research was a specially constructed, online questionnaire. The survey was completed by 244 students, 126 of whom supplement vitamin D. Pearson’s χ2 test was used to analyze the survey’s results. The processing of the obtained data, statistical calculation and graphs were made using the R program.
Results and conclusions:
Students of medical faculties are more likely to take vitamin D supplements than students of other faculties. They are significantly more likely to adjust the dose of supplementation to the current guidelines. In 87% of students who tested the level of vitamin D in the blood serum and remember their result, the level of the parameter is ≥30 ng/ml (75 nmol/L), which is below the optimal level for this particular age group. Supplementation improves the quality of life, the benefits of supplementation were noticed by 71.4% of all students. It is necessary to promote the prophylactic habit of vitamin D supplementation.
Use of a prescribed exercise intervention as an adjunct to improve venous leg ulcer healing
Authors: Quirk Kirstie 
Published: August 2023

Background: The complex healing process of venous leg ulcers (VLUs) is widely documented, yet wound healing outcomes continue to challenge community nurses. Compression therapy remains the ‘gold standard’ to improve healing outcomes. However, the complexities surrounding VLUs demand a holistic and unified approach. Advising patients with VLUs to perform exercises is a widely accepted practice based on the known benefits of lower leg mobility reducing venous hypertension. A lack of standardisation surrounding this subject has generated a rise in academic interest over the past decade, particularly in the benefit of a prescribed exercise intervention (PEI) as an adjunct to compression for VLU patients. Aim: This review explored the use of an unsupervised PEI as an adjunct to improve VLU healing in housebound patients wearing compression therapy. The aim was to determine if a PEI is beneficial to VLU healing alongside compression therapy. Results: The review identified five randomised controlled trials (RCT) between 2009 and 2022. Although the evidence showed some limitations, statistically and clinically significant results were identified for VLU healing outcomes. Conclusion: A PEI designed to engage the calf muscle pump is beneficial to improve VLU healing outcomes, alongside compression therapy, for housebound patients and should be included in the community nursing holistic model of care for VLU management.
Recyclability and catalytic characteristics of copper oxide nanoparticles derived from bougainvillea plant flower extract for biomedical application
Authors: Natrayan L. , Kaliappan S. , Saravanan A. , Vickram A. S. , Pravin P. , Abbas Mohamed , Ahamed Saleel C. , Alwetaishi Mamdooh , Saleem Mohamed Sadiq Mohamed 
Published: August 2023

Abstract
This work aims to investigate the environmentally sustainable technique to synthesize the copper nanoparticles using bougainvillea flower ethanolic extract at ambient temperature. Copper nanoparticles have considerable potential for reducing the environment’s harmful pigments and nitrogen contaminants. The oxidized copper nanoscale catalysts are enclosed inside nanomaterial, which work as a benign and sustainable resource for capping agents. Ultraviolet spectroscopic, transmission electron microscopy (TEM), and X-ray crystallography (XRD) techniques were used to evaluate the produced oxidized copper nanocrystals. The particles produced have been very robust, are cylindrical in form, and have an outer diameter of 12 nm. Furthermore, under normal conditions, copper oxide (CuO) nanomaterials demonstrated strong photocatalytic efficiency in liquid media for the oxidation of Congo red, bromothymol blue, and 4-nitrophenol in an acidic solution acetic anhydride. Moreover, the CuO nanocrystalline enzyme could be readily vortexed or used for five cycles with an exchange rate of even over 90%. The evaporation process caused around 18% of the loss of weight between 25°C and 190°C, while soil organic breakdown caused almost 31% of the loss of weight around 700°C. As a result, the little reduction in enzymatic effectiveness of the recoverable multilayer CuO substrate might be attributed to catalytic degradation throughout spinning and processing.
The PCOS puzzle: putting the pieces together for optimal care
Authors: Dar Mohd Altaf , Maqbool Mudasir , Ara Irfat 
Published: August 2023

AbstractPolycystic ovary syndrome (PCOS) is a multifaceted hormonal disorder that has significant ramifications for both women’s reproductive and metabolic well-being. This analysis aims to offer a thorough comprehension of PCOS by investigating the various contributing factors that are crucial for its effective management. We delve into the topic of hormonal imbalances, such as elevated androgens and disrupted estrogen-progesterone dynamics, and their effects on reproductive and metabolic health. Furthermore, we explore the intricate connection between insulin resistance, hyperinsulinemia, and PCOS, highlighting their pivotal role in metabolic dysfunction. Additionally, we examine fertility challenges, irregular menstrual patterns, and metabolic complications while also reviewing current treatment methodologies. Moreover, we address the latest research concerning genetic, environmental, and epigenetic influences on PCOS. By piecing together these essential elements, healthcare professionals can attain a comprehensive understanding of PCOS and deliver optimal care for those affected by the condition.
Increased risk of severe COVID-19 in pregnancy in a multicenter propensity score-matched study
Authors: Cojocaru Liviu , Noe Myint , Pahlavan Autusa , Werzen Alissa , Seung Hyunuk , Yoo Young Chae Jessica , Tyson Patricia , Narayanan Shivakumar , Turan Shifa , Turan Ozhan M. , Chua Joel V. 
Published: August 2023

Abstract

Objectives
To explore the association between COVID-19 severity and pregnancy using measures such as COVID-19 ordinal scale severity score, hospitalization, intensive care unit (ICU) admission, oxygen supplementation, invasive mechanical ventilation, and death.

Methods
We conducted a retrospective, multicenter cohort study to understand the association between COVID-19 severity and pregnancy. We reviewed consecutive charts of adult females, ages 18–45, with laboratory testing for SARS-CoV-2 infection between March 1, 2020, and August 31, 2020. Cases were patients diagnosed with COVID-19 during pregnancy, whereas controls were not pregnant at the time of COVID-19 diagnosis. Primary endpoints were the COVID-19 severity score at presentation (within four hours) and the nadir of the clinical course. The secondary endpoints were the proportion of patients requiring hospitalization, ICU admission, oxygen supplementation, invasive mechanical ventilation, and death.

Results
A higher proportion of pregnant women had moderate to severe COVID-19 disease at the nadir of the clinical course than non-pregnant women (25 vs. 16.1 %, p=0.04, respectively). There was a higher rate of hospitalization (25.6 vs. 17.2 %), ICU admission (8.9 vs. 4.4 %), need for vasoactive substances (5.0 vs. 2.8 %), and invasive mechanical ventilation (5.6 vs. 2.8 %) in the pregnant cohort. These differences were not significant after applying propensity score matching.We found a high rate of pregnancy complications in our population (40.7 %). The most worrisome is the rate of hypertensive disorders of pregnancy (20.1 %).

Conclusions
In our propensity score-matched study, COVID-19 in pregnancy is associated with an increased risk of disease severity and pregnancy complications.
Classification of self-adjoint domains of odd-order differential operators with matrix theory
Authors: Zhang Maozhu , Hao Xiaoling , Wang Jing 
Published: August 2023

Abstract
In this article, we investigate the classification of self-adjoint boundary conditions of odd-order differential operators. We obtain that for odd-order self-adjoint boundary conditions under some assumptions, there are exactly two basic types of self-adjoint boundary conditions: coupled and mixed. Moreover we determine the number of possible conditions for each type, which is different from the even-order cases. Our construction will play an important role in the canonical forms and in the spectral analysis of these operators.