bluuebunny/crossref_metadata_2025_split huggingface dataset:
A head-to-head comparison of two DREADD agonists for suppressing operant behavior in rats via VTA dopamine neuron inhibition
Authors: Lawson Kate A , Ruiz Christina M , Mahler Stephen V 
Published: March 2023

AbstractRationaleDesigner receptors exclusively activated by designer drugs (DREADDs) are a tool for “remote control” of defined neuronal populations during behavior. These receptors are inert unless bound by an experimenter-administered designer drug, most commonly clozapine-n-oxide (CNO). However, questions have emerged about the suitability of CNO as a systemically administered DREADD agonist.ObjectivesSecond-generation agonists such as JHU37160 (J60) have been developed, which may have more favorable properties than CNO. Here we sought to directly compare effects of CNO (0, 1, 5, & 10 mg/kg, i.p.) and J60 (0, 0.03, 0.3, & 3 mg/kg, i.p.) on operant food pursuit.MethodsMale and female TH:Cre+ rats and their wildtype (WT) littermates received cre-dependent hM4Di-mCherry vector injections into ventral tegmental area (VTA), causing inhibitory DREADD expression in VTA dopamine neurons in TH:Cre+ rats. Rats were trained to stably lever press for palatable food on a fixed ratio 10 schedule, and doses of both agonists were tested on separate days in a counterbalanced order.ResultsAll three CNO doses reduced operant food seeking in rats with DREADDs, and no CNO dose had behavioral effects in WT controls. The highest tested J60 dose significantly reduced responding in DREADD rats, but this dose alsoincreasedresponding in WTs, indicating non-specific effects. The magnitude of CNO and J60 effects in TH:Cre+ rats were correlated and were present in both sexes.ConclusionsFindings demonstrate the usefulness of directly comparing DREADD agonists when optimizing behavioral chemogenetics, and highlight the importance of proper controls, regardless of the DREADD agonist employed.
Evolution of an alternative genetic code in theProvidenciasymbiont of the haematophagous leechHaementeria acuecueyetzin
Authors: Manzano-Marín Alejandro , Kvist Sebastian , Oceguera-Figueroa Alejandro 
Published: March 2023

ABSTRACTStrict blood-feeding animals are confronted with a strong B vitamin deficiency. Blood-feeding leeches from the Glossiphoniidae family, similarly to haematophagous insects, have evolved specialised organs called bacteriomes to harbour symbiotic bacteria. Leeches of theHaementeriagenus have two pairs of globular bacteriomes attached to the oesophagus which house intracellular ’CandidatusProvidencia siddallii’ bacteria. Previous work analysing a draft genome of theProvidenciasymbiont of the Mexican leechHaementeria officinalisshowed that, in this species, the bacteria hold a reduced genome capable of synthesising B vitamins. In this work, we aimed to expand our knowledge on the diversity and evolution ofProvidenciasymbionts ofHaementeria. For this purpose, we sequenced the symbiont genomes of three selected leech species. We found that all genomes are highly syntenic and have kept a stable genetic repertoire, mirroring ancient insect endosymbionts. Additionally, we found B vitamin pathways to be conserved among these symbionts, pointing to a conserved symbiotic role. Lastly and most notably, we found that the symbiont ofHaementeria acuecueyetzinhas evolved an alternative genetic code, affecting a portion of its proteome and showing evidence of a lineage-specific and likely intermediate stage of genetic code reassignment.
Regulatory Variants on the Leukocyte Immunoglobulin-Like Receptor Gene Cluster are Associated with Crohn’s Disease and Interact with Regulatory Variants forTAP2
Authors: Kim Kwangwoo , Oh Shin Ju , Lee Junho , Kwon Ayeong , Yu Chae-Yeon , Kim Sangsoo , Choi Chang Hwan , Kang Sang-Bum , Kim Tae Oh , Park Dong Il , Lee Chang Kyun 
Published: March 2023

AbstractBackground and AimsCrohn’s disease (CD) has a complex polygenic etiology with high heritability. We keep putting an effort to identify novel variants associated with susceptibility to CD through a genome-wide association study (GWAS) in large Korean populations.MethodsGenome-wide variant data from 902 Korean patients with CD and 72,179 controls were used to assess the genetic associations in a meta-analysis with previous Korean GWAS results from 1,621 patients with CD and 4,419 controls. Epistatic interactions between CD-risk variants of interest were tested using a multivariate logistic regression model with an interaction term.ResultsWe identified two novel genetic associations with the risk of CD nearZBTB38and within the leukocyte immunoglobulin-like receptor (LILR) gene cluster (P<5×10−8), with highly consistent effect sizes between the two independent Korean cohorts. CD-risk variants in the LILR locus are known quantitative trait loci (QTL) for multiple LILR genes, of whichLILRB2directly interacts with various ligands including MHC class I molecules. The LILR lead variant exhibited a significant epistatic interaction with CD-associated regulatory variants forTAP2involved in the antigen presentation of MHC class I molecules (P=4.11×10−4), showing higher CD-risk effects of theTAP2variant in individuals carrying more risk alleles of the LILR lead variant (OR=0.941,P=0.686 in non-carriers; OR=1.45,P=2.51×10−4in single-copy carriers; OR=2.38,P=2.76×10−6in two-copy carriers).ConclusionsThis study demonstrated that genetic variants at two novel susceptibility loci and the epistatic interaction between variants in LILR andTAP2loci confer risk of CD.
Strategies to deliver smoking cessation interventions during targeted lung health screening - a systematic review and meta-analysis
Authors: Williams Parris J , Philip Keir EJ , Alghamdi Saeed M , Perkins Alexis M , Buttery Sara C , Polkey Michael I , Laverty Anthony A , Hopkinson Nicholas S 
Published: March 2023

ABSTRACTIntroductionLung cancer screening presents an important teachable moment to promote smoking cessation, but the most effective strategy to deliver support in this context remains to be established.MethodsWe undertook a systematic review and meta-analysis of smoking cessation interventions delivered during lung health screening, published prior to 20/07/2022 MEDLINE, PsychINFO, CENTRAL, EMBASE, CINAHL and Scopus databases. Two reviewers screened titles, and abstracts, four reviewed each full text using prespecified criteria, extracted relevant data, assessed risk of bias and confidence in findings using the GRADE criteria. The review was registered prospectively on PROSPERO (CRD42021242431).Results10 randomised controlled trials (RCT) and 3 observational studies with a control group were identified. Meta-analysis of 9 RCTs demonstrated that smoking cessation interventions delivered during lung screening programmes increased quit rates compared to usual care (OR: 2.01, 95%: 1.49-2.72 p<0.001). 6 RCTs using intensive (≥3 behavioural counselling sessions) interventions demonstrated greater quit rates compared to usual care (OR: 2.11, 95% CI 1.53-2.90, p<0.001). A meta-analysis of 2 RCTs found intensive interventions were more effective than non-intensive (OR: 2.07, 95%CI 1.26-3.40 p=0.004), Meta-analysis of 2 RCTs of non-intensive interventions (≤2 behavioural counselling sessions or limited to online information audio take home materials such as pamphlets) did not show a higher quit rate than usual care (OR: 0.90, 95% CI 0.39-2.08 p=0.80).DiscussionModerate quality evidence supports smoking cessation interventions delivered within a lung screening setting compared to usual care, with high-quality evidence that more intensive interventions are likely to be most effective.
Understanding social media users' information avoidance intention: a C-A-C perspective
Authors: Zhou Tao , Xie Yingying 
Published: March 2023

PurposeBased on the C-A-C framework, this article examined users' information avoidance intention in social media platforms.Design/methodology/approachThe authors conducted data analysis using a mixed method of the SEM and fsQCA.FindingsThe results indicated that information overload, functional overload and social overload influence fatigue and dissatisfaction, both of which further determine users' information avoidance intention. The results of the fsQCA identified two paths that trigger users' information avoidance intention.Originality/valueExtant studies have examined the information avoidance in the contexts of healthcare, academics and e-commerce, but have seldom explored the mechanism underlying users' information avoidance in social media. To fill this gap, this article will empirically investigate users' information avoidance in social media platforms based on the C-A-C framework.
Need to cut or freeze pay of your employees? Providing work–life balancing practices can help if maintaining employee morale is a concern
Authors: Yoon Yeongjoon , Sengupta Sukanya 
Published: March 2023

PurposeResearch on the effect of pay cuts/freezes on employee morale is limited. More importantly, past studies examining this relationship tend to focus on fairness perception as a mediator. This study hypothesizes that work–life conflict also mediates the negative relationship between pay cuts/freezes and employee morale.Design/methodology/approachA total of 13,139 employees in 1,830 workplaces in Britain in the 2011 Workplace Employment Relations Survey were analyzed.FindingsThe analyses confirm the above hypothesis. The results also demonstrate that this mediating mechanism can be mitigated to some extent when work–life balancing practices are available, but much more strongly when they are actually used.Practical implicationsIf possible, organizations should provide and encourage employees to use work–life balancing practices when employees' pay needs to be cut or frozen if maintaining employee morale is a concern.Originality/valueOur study highlights the need to incorporate various theoretical frameworks, and not just the dominant justice/fairness theories, into the study of pay cuts and freezes. The current research demonstrates that the work–life conflict framework can also be applied to understand the relationship between pay cuts/freezes and employee morale.
Sustainable refurbishment of existing, typical single-family residential buildings in Greece
Authors: Bougiatioti Flora , Alexandrou Eleni , Katsaros Miltiadis 
Published: March 2023

PurposeResidential buildings in Greece constitute an important portion of the existing building stock. Furthermore, most of these buildings were built prior to the first Thermal Insulation Code of 1981. The article focuses on existing, typical residences built after 1920, which are found mostly in suburban areas and settlements all around Greece. The purpose of the research is to evaluate the effect of simple bioclimatic interventions focused on the improvement of their diurnal, inter-seasonal and annual thermal performance.Design/methodology/approachThe applied strategies include application of thermal insulation in the building shell and openings, passive solar systems for the heating period and shading and natural ventilation for the summer period. The effect of the strategies is analysed with the use of building energy analysis. The simulation method was selected because it provides the possibility of parametric analysis and comparisons for different proposals in different orientations.FindingsThe results show that the increased thermal mass of the construction is the most decisive parameter of the thermal behaviour throughout the year.Research limitations/implicationsThe typical residences under investigation are often found in urban and/or suburban surroundings. These mostly refer to free-standing buildings situated, which, in many cases, do not have the disadvantages and limitations that the geometrical characteristics of densely built urban locations impose on incident solar radiation (e.g. overshadowing during the winter) and air circulation (e.g. reduce natural ventilation during the summer). Nevertheless, even in these cases, the surrounding built environment may also have relevant negative effects, which were not taken under consideration and could be included in further, future research that will include the effect of various orientations, as well as of neighbouring buildings.Practical implicationsExisting residences built prior to the first Thermal Insulation Code (1981) form an important part of the building stock. Consequently their energy upgrade could contribute to significant conventional energy savings for heating and cooling, along with the inter-seasonal improvement of interior thermal comfort conditions.Social implicationsThe proposed interventions can improve thermal comfort conditions and lead to a reduction of energy consumption for heating and cooling, which is an important step against energy poverty and the on-going energy crisis.Originality/valueThe proposed interventions only involve the building envelope and are simple with relatively low cost.
Adoption of additive manufacturing technology: drivers, barriers and impacts on upstream supply chain design
Authors: Ronchini Alessio , Moretto Antonella Maria , Caniato Federico 
Published: March 2023

PurposeThis paper investigates how the adoption of additive manufacturing (AM) impacts upstream supply chain (SC) design and considers the influence of drivers and barriers towards the adoption.Design/methodology/approachTen case studies investigating AM adoption by Original Equipment Manufacturers (OEMs) in five industries were conducted. This research is driven by a literature-based framework, and the results are discussed according to the theory of transaction cost economics (TCE).FindingsThe case studies reveal four patterns of AM adoption that affect upstream SC design (due to changes in supply base or types of buyer–supplier relationships): make, buy, make and buy and vertical integration. A make or buy decision is based on the level of experience with the technology, on the AM application (rapid manufacturing, prototyping or tooling) and on the need of control over production. Other barriers playing a role in the decision are the high initial investments and the lack of skills and knowledge.Originality/valueThis paper shows how different decisions regarding AM adoption result in different SC designs, with a specific focus on the upstream SC and changes in the supply base. This research is among the first to provide empirical evidence on the impact of AM adoption on upstream SCs and to identify drivers of the make or buy decision when adopting AM through the theoretical lens of TCE.
Temperature patterns obtained in thermoelastic stress test at different frequencies, a FEM approach
Authors: Duarte Guilherme , Neves Ana M.A. , Ramos Silva António 
Published: March 2023

PurposeThe goal of this work is to create a computational finite element model to perform thermoelastic stress analysis (TSA) with the usage of a non-ideal load frequency, containing the effects of the material thermal properties.Design/methodology/approachThroughout this document, the methodology of the model is presented first, followed by the procedure and results. The last part is reserved to results, discussion and conclusions.FindingsThis work had the main goal to create a model to perform TSA with the usage of non-ideal loading frequencies, considering the materials’ thermal properties. Loading frequencies out of the ideal range were applied and the model showed capable of good results. The created model reproduced acceptably the TSA, with the desired conditions.Originality/valueThis work creates a model to perform TSA with the usage of non-ideal loading frequencies, considering the materials’ thermal properties.
Exploring the relationship between digitalization, resilient agri-food supply chain management practices and firm performance
Authors: Yadav Sanjeev , Luthra Sunil , Kumar Anil , Agrawal Rohit , Frederico Guilherme F. 
Published: March 2023

PurposeThis study aims to explore the mediating role of digital technologies-based supply chain integrating (SCI) strategies on the agri-supply chain performance (SCP) and firm performance (FP). This research has introduced recently emerged digital technologies such as Internet of Things (IoT). Further, based on theoretical support and an extensive literature review, this research has proposed some hypotheses, which have been quantitatively validated for their significance.Design/methodology/approachA conceptual model was formulated based on an extensive literature review. Data for this research were gathered from a survey completed by 119 respondents from different departments of agri-firms. Further, partial least square (PLS)-based structured equation modelling (SEM) was used to test the proposed hypothetical model.FindingsThe results confirm that IoT-based digital technologies and supply chain processes (organization integration [OI], information sharing and customer integration [CI]) have a significant positive correlation. Furthermore, supply chain practices are positively associated with SCP. Finally, it has been found that FP is positively impacted by SCP.Research limitations/implicationsThis research is used to analyse the mediating impacts of digital supply chain processes as a linking strategy for SCP and FP. For practical purposes, this research provides investment decisions for implementing digital technologies in SC strategies. The findings have proposed implications for managers and practitioners in agri-firms based on existing theories: contingency theory (CT) and relational view theory. Also, this study suggests the deployment of smarter electronically based tags and readers, which improve the data analytics capabilities based on auto-captured data. Thus, the availability of quality information improves the data-driven decisional capabilities of managers at company level.Originality/valueThis is a unique and original study exploring the relationship between digitalization, resilient agri-food supply chain (AFSC) management practices and firm performance. This research may be extended to other industries in view of the results from SCP and impact of digitalization.
Improving decision making through visual knowledge calibration
Authors: Muntwiler Christian , Eppler Martin J. 
Published: March 2023

PurposeThis article aims to explore the so-called illusion of explanatory depth (IOED) of managers regarding their understanding of digital technologies and examines the effect of knowledge visualization one’s current understanding and decision making. Its purpose is to show that managers think they know more than they do and that this affects decision making but can be reduced through knowledge visualization.Design/methodology/approachIn two experiments with experienced managers, the authors investigate the size and impact of the IOED bias in decision making and examine if sketched self-explanations are as effective as written self-explanations to reduce the bias.FindingsThe findings show that experienced managers suffer from a significant illusion concerning their explanatory understanding of digital technologies and that sketching one’s current level of explanatory understanding of these technologies supports the accurate calibration of one’s knowledge. The findings indicate that sketching knowledge is a helpful modality for the detection and subsequent recalibration of biased knowledge in domain-dependent decision making.Originality/valueThis article is the first to explore the effect of sketched knowledge externalization on the calibration of explanatory knowledge of managers. It extends the literature on both, the IOED and on knowledge visualization as an instrument of knowledge calibration.
Managing TQM's soft side: an explorative study of social care multiservice organizations
Authors: Capolupo Nicola , Virglerová Zuzana , Adinolfi Paola 
Published: March 2023

PurposeThis paper explores total quality management (TQM) soft domain efficacy in social care organizations to determine the extent to which an organization's project success may stem from soft TQM critical success factors (CSFs).Design/methodology/approachNon-structured interviews were conducted with 16 managers overseeing the prosthetic device regeneration project of the Italian local health unit (located in Salerno) to explore which soft factors could contribute to the success of a social care multifunctional organization.FindingsOrganizations' handling of certain projects, such as pivoting on soft TQM issues, may allow them to be configured as multiservice organizations. Therefore, a conceptual model of a multiservice social care organization is proposed.Practical implicationsFrom a managerial perspective, this study presents an interesting success case of a multiservice social care organization with a total annual expenditure of €20 million on prosthetic assistance. Preliminary data show a 13% reduction in public expenditure for Salerno's local health unit via a refurbishment project.Originality/valueThe paper contributes to the soft TQM literature debate: although Italian local health professionals appear aware of soft TQM issues' implementation and consciously apply them in their organization and projects, this occurs more with specific CSFs emerging from the literature. Therefore, this article paves the way for further quantitative and theoretical investigations on the adoption of TQM soft issues in social care organizations' performance measurement.
An integrated learning framework of corporate training system: a grounded theory approach
Authors: Barile Sergio , Ciasullo Maria Vincenza , Testa Mario , La Sala Antonio 
Published: March 2023

PurposeRooting in the literature on training and laying on Kirkpatrick model, this paper aims to explore key drivers of corporate training to identify how they can be combined into an integrated framework of learning for human capital development.Design/methodology/approachBy adopting the constructivist grounded theory, this contribution analyzes the experience carried out in the last ten years by Virvelle, an Italian corporate training firm.FindingsResults show the rise of five core categories, g1iving rise to an integrated model of Kirkpatrick. Their dynamic interplay led to a new orientation of Kirkpatrick model giving rise to a metalearning ecosystem.Research limitations/implicationsManagerial implications have identified key factors on which building and implementing appropriate corporate training programmes capable of triggering co-generative processes of value creation. Particularly, the essential role of learning quality culture, digital technology and personalization are detected in integrating not only hard but furthermore soft shades of learning. Concerning theoretical implications, the emergence of key structural and systems enabling dimensions for learning, and contextual mechanisms involved in reshaping training effectiveness and achieving integrated learning outcomes are detected. The main limitation of this study lies in the need to generalize results: the conceptualized framework needs to be empirically tested.Originality/valueThe value of this research is built along three main points. The first is the integration among the core categories that an integrated learning system can be built on, promoting learning quality culture through positive feedback loops. The second is represented by the chance to enhance an integrated mutual knowledge development among engaged actors, thereby shaping a more holistic and multidimensional learning model. The third is related to the transversal role that digital technology plays in all phases of the training process as it integrates and enriches them.