bluuebunny/crossref_metadata_2025_split huggingface dataset:
Coping with drought? The hidden microhabitat selection and underground movements of amphisbaenians under summer drought conditions
Authors: Martín José , Rodríguez-Ruiz Gonzalo , Cuervo José Javier 
Published: August 2023

Abstract
Habitat selection is a dynamic process that depends on many environmental variables that can vary with weather conditions. This is important because, within a context of global change, extreme weather events, such as severe droughts, are predicted to become more frequent. We examined the patterns of microhabitat selection and underground movements (using PIT-tag telemetry) of a strictly fossorial reptile, the North African checkboard amphisbaenian Trogonophis wiegmanni, during the summer drought period. We aimed to test whether changes in strategies of habitat use and movements could allow this amphisbaenian to cope with unfavorable weather. We found that during the summer drought period, T. wiegmanni did not use the microhabitats in relationship to their availability, but particularly selected sites with high abundance of rocks but also areas under a high cover of bushes, where environmental conditions were more favorable. We also found, using PIT-tag telemetry, that the numbers of T. wiegmanni individuals located under rocks and their activity (number of days with movements) decreased largely in summer. However, the animals were not entirely inactive, but, especially males were active below the ground under bushes and made some relatively long underground hidden movements between favorable areas.
Race and Ethnic Representation in Crohn’s Disease Trials of Biologic and Small Molecule Medications: A Systematic Review and Meta-analysis
Authors: Pelton Matt , Ssentongo Paddy , Sun Ashley , Groff Destin , Dalessio Shannon , Clarke Kofi 
Published: August 2023

Abstract

Background and Aims
Randomised controlled trials historically under-represent marginalised racial and ethnic populations. As incidence and prevalence of Crohn’s disease in these groups rise, it is important to characterise their inclusion in randomised controlled trials on first-line and pipe-line medications.

Methods
PubMed was searched systematically for randomised controlled trials of biologic and small molecule inhibitor [SMI] medications, with a primary outcome related to efficacy following PRISMA guidelines. We used descriptive statistics to summarise demographic variables and meta-regression analyses to estimate temporal trends in racial inclusion.

Results
More than a half of trials did not report any racial/ethnic demographics [53.7%] and several reported racial demographics for only one race [20.9%]. When racial data were reported, Whites made up 90.2% of participants. Percentages of Black, Asian, Native American/Pacific Islander, and participants considered ‘Other’ averaged 2.9%, 11.6%, 0.5%, and 1.6% out of the total sample sizes of 3901, 3742, 828 and 4027, respectively. Proportional representation of White participants decreased over time [p &lt;0.01] and proportional representation of Asian participants increased over time [p = 0.047]. In ordinal logistic regression, mean year of trial enrolment significantly increased the number of racial groups reported [p &lt;0.001].

Conclusions
Half of published randomised controlled trials in Crohn’s disease contain no racial or ethnic demographics, and the remaining often only have limited inclusion of Black, Native American/Pacific Islander, and Hispanic patients. Further work should characterise representation in observational and prospective trials. Researchers should work to: 1] increase reporting of racial and ethnic demographics; and 2] improve recruitment and retention of marginalised populations.
Determinants and prognostic implications of left atrial reverse remodelling after coarctation of aorta repair in adults
Authors: Egbe Alexander C , Younis Ahmed , Miranda William R , Jain C Charles , Connolly Heidi M , Borlaug Barry A 
Published: August 2023

Abstract

Aims
Left atrial (LA) dysfunction and atrial fibrillation are also relatively common in adults with coarctation of aorta (COA), and the severity of LA dysfunction is associated with a higher risk of atrial fibrillation in this population. The purpose of this study was to determine whether LA function improved after COA repair (LA reverse remodelling), and the relationship between LA reverse remodelling and atrial fibrillation.

Methods and results
Retrospective cohort study of adults undergoing COA repair (2003–20). LA reservoir strain was assessed pre intervention and 12–24 months post intervention, using speckle tracking echocardiography. Incident atrial fibrillation was assessed from COA repair to last follow-up. Of 261 adults who underwent COA repair [age 37 ± 13 years; males 148 (57%)], 124 (47%) and 137 (53%) presented with native vs. recurrent COA, respectively. Of 261 patients, 231 (82%) and 48 (18%) underwent surgical and transcatheter COA repair, respectively. The LA reservoir strain increased from 32 ± 8% (pre intervention) to 39 ± 7% (post intervention), yielding a relative increase of 21 ± 5%. Older age [β ± standard error (SE) −0.16 ± 0.09 per 5 years, P = 0.02], higher systolic blood pressure (β ± SE −0.12 ± 0.04 per 5 mmHg, P = 0.005), and higher residual COA mean gradient (β ± SE −0.17 ± 0.06 per 5 mmHg, P = 0.002) post intervention were associated with less LA reverse remodelling, after adjustment for sex, hypertension diagnosis, and left ventricular indices. LA reverse remodelling (hazard ratio 0.97, 95% confidence interval 0.96–0.98 per 1% increase from pre-intervention LA function, P = 0.006) was associated with a lower risk of atrial fibrillation after adjustment for age, sex, pre-intervention LA reservoir strain, and history of atrial fibrillation.

Conclusion
COA repair resulted in improved LA function and decreased risk for atrial fibrillation, especially in patients without residual hypertension or significant residual COA gradient.
A case of ultra-prolonged intra-aortic balloon pump support via sheathless femoral access
Authors: Al-Ani Mohammad A , Snipes Garrett , Parker Alex M , Kerensky Richard A 
Published: August 2023

Abstract

Background
An intra-aortic balloon pump (IABP) is a mechanical circulatory support platform with a relatively low complication rate. Axillary access is increasingly utilized to allow rehabilitation.

Case summary
We present a case of femoral IABP inserted into the femoral artery percutaneously via a sheathless technique that allowed the patient to ambulate and physically rehabilitate over 102 days until cardiac transplantation. The patient was able to progress with the protocolized rehabilitation programme to up to 3500 ft walking distance. The IABP was removed at the time of transplantation without any vascular complications.

Discussion
While axillary IABP offers an opportunity to rehabilitate, it has an unacceptably high complication rate, often resulting in vascular injury that adds morbidity to an acutely ill cohort. In this case, we found that sheathless femoral IABP access offered stability for a prolonged time while avoiding pain, bleeding, infection, and vascular injury. We hypothesize that this is due to less indwelling prosthetic material usage and also device flexibility, allowing conformation to the natural course of the femoral artery. We are encouraged by this case to use a sheathless access approach for patients expected to require prolonged IABP support.
Is the Epithor conversion score reliable in robotic-assisted surgery anatomical lung resection?
Authors: Armand Elsa , Fourdrain Alex , Lafouasse Chloé , Resseguier Noémie , Trousse Delphine , D’Journo Xavier-Benoît , Thomas Pascal-Alexandre 
Published: August 2023

Abstract

OBJECTIVES
Despite an improvement in surgical abilities, the need for an intraoperative switch from a minimally invasive procedure towards an open surgery (conversion) still remains. To anticipate this risk, the Epithor conversion score (ECS) has been described for video-assisted thoracoscopic surgery (VATS). Our objective was to determine if this score, developed for VATS, is applicable in robotic-assisted thoracoscopic surgery (RATS).

METHODS
This was a retrospective monocentric study from January 2006 to June 2022, and data were obtained from the EPITHOR database. Patients included were those who underwent anatomic lung resection either by VATS or RATS. The ECS was calculated for all patients studied. Discrimination and calibration of the test were measured by the area under the curve and Hosmer–Lemeshow test.

RESULTS
A total of 1685 were included. There were 183/1299 conversions in the VATS group (14.1%) and 27/386 conversions in the RATS group (6.9%). Patients in the RATS group had fewer antiplatelet therapy and peripheral arterial disease. There were more segmentectomies in the VATS group. As for test discrimination, the area under the curve was 0.66 [0.56–0.78] in the RATS group and 0.64 [0.60–0.69] in the VATS group. Regarding the calibration, the Hosmer–Lemeshow test was not significant for both groups but more positive (better calibrated) for the VATS group (P = 0.12) compared to the RATS group (P = 0.08).

CONCLUSIONS
The ECS seems applicable for patients operated with RATS, with a correct discrimination but a lower calibration performance for patients operated with VATS. A new score could be developed to specifically anticipate conversion in patients operated on by RATS.
Gut bacterial communities in Atlantic bottlenose dolphins (Tursiops truncatus) throughout a disease-driven (Morbillivirus) unusual mortality event
Authors: Olmstead Alyssa R B , Mathieson Olivia L , McLellan William A , Pabst D Ann , Keenan Tiffany F , Goldstein Tracey , Erwin Patrick M 
Published: August 2023

Abstract
Gut microbiomes are important determinants of animal health. In sentinel marine mammals where animal and ocean health are connected, microbiome impacts can scale to ecosystem-level importance. Mass mortality events affect cetacean populations worldwide, yet little is known about the contributory role of their gut bacterial communities to disease susceptibility and progression. Here, we characterized bacterial communities from fecal samples of common bottlenose dolphins, Tursiops truncatus, across an unusual mortality event (UME) caused by dolphin Morbillivirus (DMV). 16S rRNA gene sequence analysis revealed similar diversity and structure of bacterial communities in individuals stranding before, during, and after the 2013–2015 Mid-Atlantic Bottlenose Dolphin UME and these trends held in a subset of dolphins tested by PCR for DMV infection. Fine-scale shifts related to the UME were not common (10 of 968 bacterial taxa) though potential biomarkers for health monitoring were identified within the complex bacterial communities. Accordingly, acute DMV infection was not associated with a distinct gut bacterial community signature in T. truncatus. However, temporal stratification of DMV-positive dolphins did reveal changes in bacterial community composition between early and late outbreak periods, suggesting that gut community disruptions may be amplified by the indirect effects of accumulating health burdens associated with chronic morbidity.
Developmental Diet Alters the Fecundity–Longevity Relationship and Age-Related Gene Expression in Drosophila melanogaster
Authors: Collins David H , Prince David C , Donelan Jenny L , Chapman Tracey , Bourke Andrew F G 
Published: August 2023

Abstract
The standard evolutionary theory of aging predicts a negative relationship (trade-off) between fecundity and longevity. However, in principle, the fecundity–longevity relationship can become positive in populations in which individuals have unequal resources. Positive fecundity–longevity relationships also occur in queens of eusocial insects such as ants and bees. Developmental diet is likely to be central to determining trade-offs as it affects key fitness traits, but its exact role remains uncertain. For example, in Drosophila melanogaster, changes in adult diet can affect fecundity, longevity, and gene expression throughout life, but it is unknown how changes in developmental (larval) diet affect fecundity–longevity relationships and gene expression in adults. Using D. melanogaster, we tested the hypothesis that varying developmental diets alters the directionality of fecundity–longevity relationships in adults, and characterized associated gene expression changes. We reared larvae on low (20%), medium (100%), and high (120%) yeast diets, and transferred adult females to a common diet. We measured fecundity and longevity of individual adult females and profiled gene expression changes with age. Adult females raised on different larval diets exhibited fecundity–longevity relationships that varied from significantly positive to significantly negative, despite minimal differences in mean lifetime fertility or longevity. Treatments also differed in age-related gene expression, including for aging-related genes. Hence, the sign of fecundity–longevity relationships in adult insects can be altered and even reversed by changes in larval diet quality. By extension, larval diet differences may represent a key mechanistic factor underpinning positive fecundity–longevity relationships observed in species such as eusocial insects.
Heterogeneity in Measures and Rates of Reported Dementia and Subjective Memory Complaints Across U.S. National Surveys
Authors: Picchiello Matthew C , Carpenter Brian D 
Published: August 2023

Abstract

Objectives
Several U.S. health surveillance surveys contain items related to self- and proxy reports of dementia and subjective memory complaints (SMC). Despite their similar content, these items differ in terminology, item specificity, and time frame. The goal of this study was to analyze whether item features might influence endorsement rates for dementia and SMC.

Methods
We calculated design-appropriate estimates for the endorsement of dementia and SMC across U.S.-based national surveys and employed pairwise comparisons to evaluate endorsement rates across surveys. We also examined item characteristics to explore possible effects on endorsement rates.

Results
Endorsement rates were wide-ranging for dementia (ranging from 2.7% to 9.9%) and SMC (5.6% to 46.6%). Pairwise comparisons revealed statistically significant differences on most dementia-related items (76%), and all SMC comparisons (100%). Items varied substantially in the terminology used to assess dementia and SMC (e.g., “dementia” vs “Alzheimer’s disease”) and used different time frames (e.g., “past month” vs “5 years”).

Discussion
National survey data on reported dementia and SMC can have important research, training, and policy implications, yet endorsement rates vary widely across surveys. That variability could emerge from subtle but influential item characteristics, and our findings highlight the need for item harmonization, in even their most basic characteristics. Standardizing items across national surveillance surveys facilitates comparison across surveys so that we can better understand the true burden of these conditions to inform public health initiatives.
Does Age Matter? Tweets About Gerontocracy in the United States
Authors: Ng Reuben , Indran Nicole 
Published: August 2023

Abstract

Objectives
Discussions on the political scene in the United States have been accompanied by the notion that the country has morphed into a gerontocracy, a system of governance where power is wielded predominantly by older individuals. With rising life expectancy, more as well as older candidates are likely to run for and stay in office. This study analyzes perceptions regarding the older political leadership of the United States.

Methods
We collected 3,599 tweets containing the word “gerontocracy” which were uploaded between September 10, 2022 and November 10, 2022, a month prior to the midterm elections in the United States, when this topic was likely to have sparked considerable discussion. After applying our exclusion criteria, 1,266 tweets were retained. Our qualitative content analysis was performed inductively and deductively.

Results
Seven themes emerged. Most tweets (24%; N = 304) highlighted that “Gerontocracy is a Problem” (Theme 1). Theme 2 (21%) focused on how “Democracy is a Sham” (N = 270). Theme 3 (19%) positioned “Old Age as the Problem and Youth as the Solution” (N = 234). Theme 4 (11%) was about how “The Future is at Stake” (N = 145). “Health Concerns” dominated Theme 5 (10%; N = 126). Theme 6 looked at “Structural Reforms” (9%, N = 115). Theme 7 “In Defense of the Gerontocracy” formed 6% of the data set (N = 72).

Discussion
Attitudes toward older politicians are commonly tied to negative stereotypes surrounding old age. As the population ages, it is paramount that society focuses on potentially more important considerations in the evaluation of the actual caliber of a politician.
A single-step, rapid, and versatile method for simultaneous detection of cell surface glycan profiles using fluorochrome-conjugated lectins
Authors: Torres Nicolás I , Manselle Cocco Montana N , Perrotta Ramiro M , Mahmoud Yamil D , Salatino Mariana , Mariño Karina V , Rabinovich Gabriel A 
Published: August 2023

Abstract
Cell surface glycans play essential roles in diverse physiological and pathological processes and their assessment has important implications in biomedicine and biotechnology. Here we present a rapid, versatile, and single-step multicolor flow cytometry method for evaluation of cell surface glycan signatures using a panel of selected fluorochrome-conjugated lectins. This procedure allows simultaneous detection of cell surface glycans with a 10-fold reduction in the number of cells required compared with traditional multistep lectin staining methods. Interestingly, we used this one-step lectin array coupled with dimension reduction algorithms in a proof-of-concept application for discrimination among different tumor and immune cell populations. Moreover, this procedure was also able to unveil T-, B-, and myeloid cell subclusters exhibiting differential glycophenotypes. Thus, we report a rapid and versatile lectin cytometry method to simultaneously detect a particular repertoire of surface glycans on living cells that can be easily implemented in different laboratories and core facilities.
Mutations in human DNA methyltransferase DNMT1 induce specific genome-wide epigenomic and transcriptomic changes in neurodevelopment
Authors: Davis Kasey N , Qu Ping-Ping , Ma Shining , Lin Ling , Plastini Melanie , Dahl Niklas , Plazzi Giuseppe , Pizza Fabio , O’Hara Ruth , Wong Wing Hung , Hallmayer Joachim , Mignot Emmanuel , Zhang Xianglong , Urban Alexander E 
Published: August 2023

Abstract
DNA methyltransferase type 1 (DNMT1) is a major enzyme involved in maintaining the methylation pattern after DNA replication. Mutations in DNMT1 have been associated with autosomal dominant cerebellar ataxia, deafness and narcolepsy (ADCA-DN). We used fibroblasts, induced pluripotent stem cells (iPSCs) and induced neurons (iNs) generated from patients with ADCA-DN and controls, to explore the epigenomic and transcriptomic effects of mutations in DNMT1. We show cell type–specific changes in gene expression and DNA methylation patterns. DNA methylation and gene expression changes were negatively correlated in iPSCs and iNs. In addition, we identified a group of genes associated with clinical phenotypes of ADCA-DN, including PDGFB and PRDM8 for cerebellar ataxia, psychosis and dementia and NR2F1 for deafness and optic atrophy. Furthermore, ZFP57, which is required to maintain gene imprinting through DNA methylation during early development, was hypomethylated in promoters and exhibited upregulated expression in patients with ADCA-DN in both iPSC and iNs. Our results provide insight into the functions of DNMT1 and the molecular changes associated with ADCA-DN, with potential implications for genes associated with related phenotypes.
Sex and Biology: Broader Impacts Beyond the Binary
Authors: Sharpe Sam L , Anderson Andrew P , Cooper Idelle , James Timothy Y , Kralick Alexandra E , Lindahl Hans , Lipshutz Sara E , McLaughlin J F , Subramaniam Banu , Weigel Alicia Roth , Lewis A Kelsey 
Published: August 2023

Synopsis
What are the implications of misunderstanding sex as a binary, and why is it essential for scientists to incorporate a more expansive view of biological sex in our teaching and research? This roundtable will include many of our symposium speakers, including biologists and intersex advocates, to discuss these topics and visibilize the link between ongoing reification of dyadic sex within scientific communities and the social, political, and medical oppression faced by queer, transgender, and especially intersex communities. As with the symposium as a whole, this conversation is designed to bring together empirical research and implementation of equity, inclusion, and justice principles, which are often siloed into separate rooms and conversations at academic conferences. Given the local and national attacks on the rights of intersex individuals and access to medical care and bodily autonomy, this interdisciplinary discussion is both timely and urgent.
Changes in Inflammatory Cytokine Levels in Rectal Mucosa Associated With Neisseria gonorrheae and/or Chlamydia trachomatis Infection and Treatment Among Men Who Have Sex With Men in Lima, Peru
Authors: Clark Jesse L , Oldenburg Catherine E , Passaro Ryan C , Segura Eddy R , Godwin William , Fulcher Jennifer A , Cabello Robinson 
Published: August 2023

Abstract

Background
Neisseria gonorrheae and Chlamydia trachomatis are associated with mucosal inflammation and human immunodeficiency virus 1 (HIV-1) transmission. We assessed levels of inflammatory cytokines in men who have sex with men (MSM) with and without rectal gonorrhea and/or chlamydia in Lima, Peru.

Methods
We screened 605 MSM reporting condomless receptive anal intercourse for rectal N. gonorrheae/C. trachomatis using nucleic acid testing. We identified 101 cases of gonorrhea and/or chlamydia and randomly selected 50 N. gonorrheae/C. trachomatis positive cases and matched 52 negative controls. We measured levels of IL-1β, IL-6, IL-8, and TNF-α in rectal secretions. Tests for HIV-1, rectal N. gonorrheae/C. trachomatis, and mucosal cytokines were repeated after 3 and 6 months. Cytokine levels in cases and uninfected controls were compared using Wilcoxon rank-sum tests and linear regression.

Results
MSM with gonorrhea/chlamydia had elevated levels of all cytokines in rectal mucosa compared with matched controls (all P values &lt;.001). Following antibiotic treatment there were no significant differences in cytokine levels at 3- or 6-month follow-up evaluations (all P values &gt;.05).

Discussion
Rectal gonorrhea/chlamydia infection is associated with transient mucosal inflammation and cytokine recruitment. Our data provide proof of concept for rectal sexually transmitted infection screening as an HIV prevention strategy for MSM.
Clinical Trials Registration. NCT03010020.