bluuebunny/crossref_metadata_2025_split huggingface dataset:
Identification of proteins related to SIS3 by iTRAQ and PRM-based comparative proteomic analysis in cisplatin-induced acute kidney injury
Authors: Huang Jiayan , Ye Jian , Gao Yi , Wang Yu , Zhao Qing , Lou Tanqi , Lai Weiyan 
Published: September 2023

Abstract
Cisplatin is a commonly nephrotoxic drugs, causing acute kidney injury (AKI). In this study, we aimed to explore the potential regulatory role of the Smad3 phosphorylation inhibitor SIS3 in cisplatin-induced AKI. The cisplatin-induced AKI mouse model was established and treated with SIS3.Using isobaric tags for relative and absolute quantification(iTRAQ) to search for differentially expressed proteins (DEPs) and parallel reaction monitoring (PRM) to verify key DEPs. Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG) and Protein-Protein Interaction Networks (PPI) analysis were performed for DEPs. Lipid droplets in cells were observed by oil red O staining and bodipy493/503 staining. Malondialdehyde (MDA) and reactive oxygen species (ROS) levels in cells were detected by commercial kits. The protein expression levels were detected by western blot or immunohistochemistry. Proteomic analysis showed that the identified DEPs were mainly enriched in energy metabolism pathways, especially in lipid metabolism. After applying SIS3 to inhibit the phosphorylation of Smad3, the expression of NDRG1 and fatty acid oxidation key proteins CPT1A and PPARα increased, the expression of lipid synthesis related proteins SREBF1 and SCD1 decreased and the production of lipid droplets, MDA and ROS decreased. In conclusion, SIS3 may alleviate oxidative stress, reduced lipid accumulation and promoted fatty acid oxidation through NDRG1 in cisplatin-induced AKI.
Mitag-Leffler-Hyers-Ulam Stability of Euler-Cauchy Differential Equation Using Laplace Transform
Authors: Ahmed Abdel Baset 
Published: September 2023

Abstract
In this paper, we study the Mittag-Leffler-Hyers-Ulam stability and Mittag-Leffler-Hyers-Ulam-Rassias stability of the Euler-Cauchy differential equation using Laplace transform. Basically, for the first time, the Mittag-Leffler-Hyers-Ulam stability of second order differential equation with variable coefficients has been studied through the Laplace transforms. We develop this approach to identify the necessary conditions that the eigenvalue Strum-Liouville equation is Mittag-Leffler-Hyers-Ulam stable.
Mathematics Subject Classification (2010): 34K20 , 26D10 , 44A10 , 39B82 , 34A40 , 39A30.
Integration of bulk RNA sequencing and single-cell RNA sequencing reveals new natural killer cells promising as biomarkers for meningiomas and glioblastomas
Authors: Zhu Rongkun , Zhou Hongjian , Shi Jun , Bai Shanwang , Ge Ling , Lin Yi , Yin Wenhao , Zeng Hui , Wang Xiongwei 
Published: September 2023

Abstract
BACKGROUND: Meningioma (MND) and glioblastoma (GBM) are two of the most common tumors in the central nervous system. However, the exploration of common genetic features and molecular mechanisms between MND and GBM is unclear.
METHODS: we conducted an analysis of single-cell sequencing profiles on MND and GBM. Our main focus was on the expression of NK cell-associated genes, which were examined for intercellular communication analysis, cell cycle analysis, and GSVA enrichment analysis. To validate our findings, we used the GEO transcriptome dataset to compare immune-related gene profiles of MND and GBM patients. Furthermore, we delved into the characterization and immune molecular mechanisms of NK cell-related genes that were co-expressed in both diseases.
RESULTS:In this study, we analyzed single-cell sequencing data from 3 glioma patients and 3 meningioma patients to investigate the role of NK cells. We identified and tagged 108 shared NK cell marker genes. To validate NK cell expression, we used the CIBERSORT immune infiltration assay. The GSVA results indicated that both diseases showed high expression in angiogenesis and coagulation. Additionally, we employed the PPI interaction network and the Cyto-Hubba algorithm to screen 19 Hub genes. The differential expression and immune infiltration of these genes were further validated in GSE21354 and GSE43290 datasets, and the AUC value for each gene was calculated. Finally, we constructed a TF-miRNA-mRNA regulatory network based on these Hub genes
CONCLUSION: This study identifies shared NK cell marker genes between glioblastoma (GBM) and meningioma (MND) and confirms a new NK cell as a biomarker for GBM and MND.
Perceived effects of health status on sexual activity in patients with axial spondylarthritis followed for 5 years
Authors: Rohde Gudrun , Berg Kari Hansen , Pripp Are Hugo , Haugeberg Glenn 
Published: September 2023

Abstract
Background
Axial spondyloarthritis (ax-SpA) causes pain, fatigue, stiffness, loss of physical function, and poor health status, which can influence sexual activity and enjoyment.
Aims
To explore whether patients with ax-SpA perceive that their health status affects their sexual activity and to identify predictors of these perceived effects on sexual activity after a 5-year follow-up.
Methods
Data about demographics, disease, medication, health-related quality of life (HRQOL), and sexual quality of life (SQOL) were collected at the baseline and 5-year follow-up. The perceived effect of health status on sexual activity was measured by question 15 in the 15D questionnaire. Data were analysed using the McNemar and independent paired t tests and logistic regression.
Results
In the 244 patients with ax-SpA (30% women, 70% men; mean age, 46 years), measures reflecting disease activity decreased and comorbidities increased, and more patients were treated with biological drugs at 5 years. Compared with patients whose health status had little/no effect on sexual activity, those who perceived that their health status had a large effect on sexual activity were older, exercised less, fewer were employed, had more comorbidities, higher disease activity, and lower HRQOL and SQOL. The baseline predictors of a negative effect of health status on sexual activity were old age and low SQOL.
Conclusion
Patients reporting that their health status had a large effect on sexual activity at 5 years were older and had a less healthy lifestyle, more disease activity, and lower HRQOL and SQOL.
Preparation and characterization of sulfamic acid-functionalized Fe3O4 nanoparticles as an efficient magnetic nanocatalyst for the facile and eco-friendly synthesis of quinoxalines, benzothiazoles and benzoxazoles
Authors: Tajbakhsh Mahmood , Mazhari Fatemeh , Nirouei Negar 
Published: September 2023

Abstract
In this study, sulfamic acid-functionalized Fe3O4 nanoparticles (Fe3O4@EDA-SO3H) was successfully synthesized as an effective nanomagnetic acid catalyst. This magnetically recoverable catalyst was well characterized by FT-IR, XRD, TGA, VSM, SEM, EDX and CHN techniques. The prepared catalyst exhibited high efficiency in the synthesis of quinoxaline, benzothiazole and benzoxazole derivatives via simple and environmentally-benign procedure. The catalyst can be readily separated from the reaction medium by employing an external magnet and reused several times without significant loss of its catalytic activity.
Cerebrospinal Fluid Circulating Tumor DNA Genotyping and Survival Analysis in Lung Adenocarcinoma With Leptomeningeal Metastases
Authors: Bai Kaixuan , Chen Xin , Qi Xuejiao , Zhang Yu , Zou Yueli , Li Jian , Yu Lili , Li Yuanyuan , Jiang Jiajia , Yang Yi , Liu Yajing , Feng Shuanghao , Bu Hui 
Published: September 2023

Abstract
Purpose
The prognosis of patients with leptomeningeal metastasis (LM) remains poor. Circulating tumor DNA (ctDNA) has been proven more abundantly present in the cerebrospinal fluid (CSF), hence, its clinical implication as a biomarker need to be further verified.
Methods
We conducted a retrospective study of 35 lung adenocarcinoma (LUAD) patients with LM, and all patients collected matched CSF and plasma samples. All paired samples underwent next-generation sequencing (NGS) of 139 lung cancer-associated genes. The clinical characteristics and genetic profiling of LM were analyzed association with survival prognosis.
Results
LM showed genetic heterogeneity, which CSF had higher detection rate of ctDNA (P = 0.003), more median mutations count (P < 0.0001), higher frequencies of driver mutations (P < 0.01), more copy number variations (CNVs) alterations (P < 0.001) than plasma. The mutation frequency of EGFR, TP53, CDKN2A, MYC and CDKN2B genes were easier to be detected in CSF than in LUAD tissue (P < 0.05), this may reveal the underlying mechanism of LM metastasis. CSF ctDNA is helpful to analyze the mechanism of EGFR-TKIs resistance. In cohort 1, who receive 1/2 EGFR-TKIs before the diagnosis of LM, TP53 and CDKN2A were the most common EGFR-independent resistant mutations. In cohort 2, who progressed after osimertinib and developed LM, 7 patients (43.75%) had EGFR CNV detected in CSF but not plasma. Furthermore, patient characteristics and various genes were included for interactive survival analysis. Patients with EGFR gene mutation in lung tissue (P = 0.042) had higher median OS and CSF ctDNA mutation with TERT (P = 0.013) indicated lower median OS. Lastly, we reported a LM case whose CSF ctDNA dynamic changes were well correlated with his clinical treatment.
Conclusions
CSF ctDNA could provide a more comprehensive genetic landscape of LM, which indicated the potential metastasis-related and EGFR-TKIs resistance mechanisms of LM patients. Besides, genotyping of CSF combined with clinical outcomes can predict the prognosis of LUAD patients with LM.
Microalgae Chlorella vulgaris 211/11j as a promising strain for low temperature climate
Authors: Ievina Baiba , Romagnoli Francesco 
Published: September 2023

Abstract
Microalgae are regarded as a promising source of renewable energy and high-value compounds. To date most large-scale microalgae cultivation is located in hot low-latitude regions; however, high temperature and high solar intensity create a risk for overheating during summer days. Here we explore the option of microalgae cultivation at low temperature regions. The impact of low temperature on microalgae growth is rarely considered in studied species and strains. To assess the optimum temperature and to test the ability to grow in low temperatures, we tested the effect of environmental temperatures ranging from 8 to 32°C on the growth of the green microalgae Chlorella vulgaris 211/11j. This strain was selected as a candidate strain for outdoor cultivation after an extensive literature review. The results indicate that C. vulgaris 211/11j has a lower optimal growth temperature than some other strains of C. vulgaris and exhibits optimal growth over a wide temperature range from 20 to 28°C. Moreover, we demonstrate that the strain can grow successfully at low environmental temperatures below the optimum of this strain. Therefore, we suggest that C. vulgaris 211/11j may be suitable for cultivation in outdoor open ponds exposed to changing environmental temperatures in cooler climate regions.
Loss of direct vascular contact to astrocytes in the hippocampus as an initial event in Alzheimer’s disease. Evidence from patients, in vivo and in vitro experimental models.
Authors: Pomilio C , Presa J , Oses C , Vinuesa A , Bentivegna Melisa , Gregosa Amal , Riudavets Miguel , Sevlever Gustavo , Galvan Veronica , Levi Valeria , Beauquis Juan , Saravia Flavia 
Published: September 2023

Abstract
Alzheimer's disease (AD) is characterized by the accumulation of aggregated amyloid peptides in the brain parenchyma and also around vasculature. The hippocampus -a complex brain structure with a crucial role in learning and memory- is considered a target in the pathology. However, there is scarce information regarding vascular changes during the AD neurodegenerative process in this vulnerable structure, that is a unique in terms of vasculature features. Our aim was to evaluate hippocampal vascular alterations in AD patients and PDAPP-J20 mice -model of AD- and define the impact of Aβ40 and Aβ42 on endothelial activation. We found loss of physical astrocyte-endothelium interaction in the hippocampus of AD subjects as compared to non-AD donors together with decreased vascular density. Astrocyte-endothelial interactions and levels of the tight junction protein occludin were early altered in PDAPP-J20 mice, before vascular morphological changes or blood-brain barrier disruption were evident. At later stages, PDAPP-J20 mice showed decreased hippocampal vascular density and extravasation of fluorescent tracers, indicating vascular and BBB dysfunction. In vitro studies showed that exposure of human brain microvascular endothelial cells (HBMEC) to soluble Aβ40 was sufficient to promote NFκB translocation to the nucleus, leading to a reduction in occludin levels. These changes were prevented by treatment of HBMEC with an inhibitor of the membrane receptor for advanced glycation endproducts (RAGE). Additional results suggest that Aβ42 acts indirectly on the endothelium by inducing astrocytic factors. Furthermore, our results from human and mouse brain samples provide evidence for the crucial involvement of the hippocampal vasculature during Alzheimer's disease.
Efficacy of intranasal administration of dexmedetomidine in combination with midazolam for sedation in infant with cleft lip and palate: A double blinded prospective randomized trial
Authors: Wang Xiaodong , Ma Lian , Yang Xudong , Zhou Yi , Zhang Xiang , Han Fang 
Published: September 2023

Abstract
Objectives
This study aimed to evaluate the efficacy of intranasal administration of dexmedetomidine combined with midazolam as a sedative option for infants with cleft lip and palate.
Materials and Methods
Infants scheduled for cleft lip and palate repair surgery were randomly assigned to the IND group (intranasal dexmedetomidine 2 µg/kg alone) and the INDM group (intranasal dexmedetomidine 2 µg/kg combined with midazolam 0.05 mg/kg). The primary outcome was the proportion of infants underwent successful computed tomography scans under intranasal sedation. The secondary outcomes included onset time and duration of sedation, recovery time and so on.
Results
105 infants were included in the analysis. The proportion of infants underwent successful CT scans under sedation was significantly greater in the INDM group than in the IND group (47 [95.9%] vs. 45 [80.4%], p = 0.016). Additionally, the INDM group had a shorter onset time and a longer duration of sedation statistically (12 [8.5, 17] min vs. 16 [12, 20] min, p = 0.001; 80 [63.6, 92.5] min vs. 68.5 [38, 89] min, p = 0.014, respectively), and their recovery time was significantly longer (43 [30, 59.5] min vs. 31.5 [20.5, 53.5] min, p = 0.006).
Conclusion
Intranasal administration of dexmedetomidine combined with midazolam resulted in higher sedation success in comparison with sole dexmedetomidine.
Clinical Relevance:
Intranasal administration of dexmedetomidine combined with midazolam can be used for sedation in infants with cleft lip and palate imaging procedure and provide a faster onset time.
Clinical Trial Registration site: https://www.chictr.org.cn.
Clinical Trial Registration number: ChiCTR2100049122.
The Synergistic Effect Study of Lipopolysaccharide (LPS) and A53T-α-synuclein: Intranasal LPS Exposure on the A53T-α-synuclein Transgenic Mouse Model of Parkinson's Disease
Authors: He Qing , Zhang Shuzhen , Wang Jian , Ma Tengfei , Ma Ding , Wu Li , Zhou Mengxi , Zhao Lei , Chen Yajing , Liu Jianren , Chen Wei 
Published: September 2023

Abstract
Aging and the interaction between genetic and environmental factors are believed to be involved in chronic Parkinson's Disease (PD) progression. Abnormal aggregated α-synuclein is the main component of Lewy body in PD patients. The intranasal route is believed to be a gate way to the brain which facilitates entry of environmental neurotoxin into the brain and account for the smell loss in early PD. In this study, we chronically applied intranasal lipopolysaccharides (LPS) exposure on 4-month-old, 8-month-old, 12-month-old and 16-month-old A53T-α-synuclein (A53T-α-Syn) transgenic C57BL/6 mice every other day for 2 months to evaluate the behavioral, pathological, biochemical changes as well as microglial activation in these mice. We observed intranasal LPS exposed A53T-α-Syn mice displayed a robust progressive olfactory disorder, hypokinesia, selective loss of dopaminergic neurons, reduction in striatal dopamine (DA) content, and accelerated α-synuclein aggregation in the SN in an age-dependent way. Furthermore, we found obvious NF-кB activation, Nurr1 inhibition, IL-1β and TNF-α generation in microglia in the SN. By contrast, these PD-like changes were mild in WT and moderate in A53T-α-Syn mice in old-aged mice. This study demonstrated the synergistic effect of intranasal LPS and α-synuclein burden on PD progression. The potential mechanism was attributed to the inhibition of Nurr1 in microglial cells and the amplified neuroinflammation in the CNS. These multi-hit animals (aging, α-synuclein mutation and neuroinflammation) might help us to investigate the mechanisms through which mutant α-synuclein and inflammation work in concert to mediate PD neurodegeneration.
Relationship between Platelet-to-Lymphocyte Count Ratio and Acute Kidney Injury following Surgery of Stanford Type A Aorta Dissection
Authors: Ding Xiaoyan , Chen Yuanxiang , Zeng Lijuan , zheng xiao'e , zhou xiaofen 
Published: September 2023

Abstract
Objective This study aimed to evaluate the correlation between the hematological index of platelet to lymphocyte ratio (PLR) and the occurrence of acute kidney injury (AKI) following surgery of Stanford acute type A aorta dissection (ATAAD).Method We analyzed the perioperative data of the patients with ATAAD who were treated surgically in Fujian Provincial Hospital from December 2016 to December 2021.Results Patients were divided into acute AKI (n = 66) and non-AKI (n = 36) groups based on the Kidney Disease: Improving Global Outcomes (KDIGO). There was no difference in preoperative PLR values. In addition, the PLR within 24 h (T1 PLR) following surgery was higher in the AKI group than in the non-AKI group (P = 0.001). Reintubation rates, intensive care unit(ICU) stay, and 30-day mortality were significantly different in the AKI group (P = 0.004, P = 0.002 and P = 0.002). Furthermore, multivariate analyses showed that higher body mass index (BMI), hypoalbuminemia, reduced T1 PLR and preoperative renal function insufficient were risk factors for postoperative AKI. Receiver operating characteristic (ROC) curve analysis revealed that the cut-off level of T1 PLR decline predicted AKI:144.6, the area under the curve (AUC):0.7146, 0.6112–0.8181, and P = 0.0004, with a sensitivity of 58.33% and a specificity of 77.27%.Conclusions This retrospective study demonstrated that a decreased T1 PLR after surgery is the independent risk factor for the occurrence of postoperative AKI in ATAAD patients and has possible predictive value for AKI.
Resilient Ranking of Healthcare Suppliers Amid COVID-19 using Enhanced MADM with Lq* q-Rung Orthopair Multi-Fuzzy Soft Information
Authors: Mahalakshmi P. , Jayakumar Vimala , Edalatpanah S.A. , Banu K. Ashma 
Published: September 2023

Abstract
The global healthcare systems have encountered unparalleled difficulties due to the COVID-19 pandemic, underscoring the crucial significance of effective management within healthcare supply chains. This research contributes to the field of healthcare supply chain management by presenting a robust MADM methodology called lattice ordered(Lq *) q-rung orthopair multi-fuzzy soft set(Lq * q-ROMF S-MADM) for supplier evaluation and ranking amidst the challenges posed by the COVID-19 pandemic. Taking inspiration from multi-fuzzy soft set and q-rung orthopair fuzzy set, the present research article proposes a novel framework known as Lq * q-rung orthopair multi-fuzzy soft set (Lq * qROMF SS), which incorporates lattice ordering in q-rung orthopair multi-fuzzy soft set. The effectiveness of the proposed model is confirmed through successful experimentation on various important operations, including union, intersection, complement, restricted union and intersection. Moreover, the verification of De Morgan’s laws for Lq * qROMF SS is carried out specifically for these operations mentioned above. To highlight the significance of the proposed Lq * qROMF SS , a multi-attribute decision-making (MADM) problem is presented, showcasing its application in the domain of healthcare supply chain management. Furthermore, a comparative analysis is conducted to elucidate the advantages of this model in comparison to existing models.
Human papillomavirus infections among women with cervical lesions and cervical cancer in Yueyang, China: A cross-sectional study of 3674 women from 2019 to 2022
Authors: Zeng Min , Zhang Xiaoyun , He LiLi , Liu Xin , Liu Huawen , Deng Rui , Qiu Bo , Liu Fang , Xiao Hang , Li Quanlv , Li Wen , Liu Chongmei , Ge Yangqing 
Published: September 2023

Abstract
PURPOSE
To investigate the distribution of the incidence and genotypes of human papillomavirus (HPV) among women with cervical cancer (CC) and precancerous cervical lesions in Yueyang City, China, to develop prevention and control strategies for CC.
METHODS
3674 patients with cervical lesions diagnosed by pathology as a gold standard at 7 hospitals in Yueyang City between September 2019 and September 2022 were selected. They included 1910, 718, 576, and 470 cases of cervical intraepithelial neoplasia (CIN) I, CIN II, CIN III, and CC, respectively. A cross-sectional study combining HPV test results from these cases.
RESULTS
The total HPV prevalence was 74.69% (95%CI: 73.28–76.09%) in 3674 patients. The incidence of high- and low-risk HPV was 73.46% and 7.21%, respectively. The prevalence of HPV in CIN I, CIN II, CIN III, and invasive CC (ICC) groups was 66.65% (1273/1910, 95%CI: 64.53–68.77%), 80.78% (580/718, 95%CI: 77.89–83.67%), 83.88% (483/576, 95%CI: 80.84–86.87%), and 86.81% (408/470, 95%CI: 83.74–89.88%), respectively. The top three HPV subtypes in ICC are HPV16, HPV52, and HPV58. The prevalence of HPV 16 increased with increasing disease severity, with this genotype being present in 12.57%, 20.89%, 36.98%, and 50.85% of CIN I, CIN II, CIN III, and ICC cases, respectively (p < 0.001). Single HPV infection was predominant in cervical lesions, with a prevalence of 48.50% (95%CI: 46.89–50.12%). The HPV prevalence varied by age, being highest among women with ICC, CIN I, CIN II and CIN III aged ≥ 60 years, 50 to 59 years, 40 to 49 years, and 40 to 49 years, respectively.
CONCLUSION
The prevalence of HPV in patients with cervical lesions in Yueyang City was very high, with HPV 16, 52, 58, 53, and 51 being the five most common HPV genotypes in patients with cervical lesions. These results serve as data for supporting future vaccination and screening programs.