bluuebunny/crossref_metadata_2025_split huggingface dataset:
Thermo-responsive tri-modal antimicrobial platform for precise biofilm removal and enhanced wound healing
Authors: Du Ting , Cao Jiangli , Huang Yachen , Li XingXing , Wei Lifei , Xiao Zehui , Jiao Jingbo , Song Zhiyong , Liu Jifeng , Du Xinjun , Wang Shuo 
Published: June 2023

Abstract
A bacterial-specific therapeutic nanosystem (HMAPH) is developed based on biodegradable hollow mesoporous Prussian blue encapsulating photosensitizer methylene blue (MB), followed by modification with gold nanoparticles, polymyxin B (PMB), and hyaluronic acid (HA) to improve near-infrared (NIR) response release characteristics, specificity, and hydrophilicity. In this system, hyaluronic acid is decomposed under the action of hyaluronidase, and the exposed nanomaterials specifically bind the outer membrane of Pseudomonas aeruginosa (P. aeruginosa) through PMB to increase the membrane sensitivity to photodynamic treatment. Under dual light irradiation, a large amount of iron ions released by HMAPH can undergo Fenton reaction with H2O2 in bacteria to generate hydroxyl radicals (▪OH), enabling photothermal properties to directly kill cells by hyperthermia. Additionally, the photodynamic activity of MB released by photoinduced activation can generate reactive oxygen species, achieving synergistic and effective inhibition of P. aeruginosa. HMAPH can also inhibit and disrupt biofilm formation and down-regulate the expression of virulence factors. In vivo experiments revealed that HMAPH can accelerate P. aeruginosa-infected wound healing by promoting angiogenesis and skin regeneration, inhibiting inflammatory response, and promoting M1 to M2 polarization. Our work proposes a strategy against bacteria and biofilms through a synergistic photothermal-photodynamic-Fenton reaction, opening up new prospects for combating biofilm-associated infections.
In situ HER2 RNA expression as a predictor of pathologic complete response of HER2- positive breast cancer patients receiving neoadjuvant chemotherapy and anti-HER2 targeted treatment
Authors: Lien Huang-Chun , Lo Chiao , Lee Yi-Hsuang , Lin Po-Hang , Wang Ming-Yang , Kuo Wen-Hung , Tsai Li-Wei , Lu Yen-Shen , Hu Hsiang-Wei , Li Yu-Chia , Huang Chiun-Sheng 
Published: June 2023

Abstract

Background
Immunohistochemistry (IHC) and in situ hybridization (ISH) remain standard biomarkers for therapeutic decisions in human epidermal growth factor 2 (HER2)-positive breast cancers (BCs); however, they are insufficient to explain the heterogeneous anti-HER2 response.
Methods
We aimed to investigate the correlation of in situ HER2 RNA expression (isHRE), using RNAscope, with HER2 biomarkers and the impact of isHRE on the pathological complete response (pCR) rates of 278 patients with HER2 IHC/fluorescence ISH (FISH)-positive BC receiving neoadjuvant chemotherapy and anti-HER2 targeted treatment (NCTT).
Results
We validated HER2 RNAscope scoring as a semiquantitative method to determine isHRE and showed a positive correlation between RNAscope scores and pCR rates, with particularly different rates between patients with a score of 5 versus 1–4 BCs (66.7% vs. 15.9%, p < 0.0001). There were higher RNAscope scores and pCR rates in patients with HER2 IHC 3 + versus IHC 2+/FISH + BCs and HER2 RNAscope scores and pCR rates showed similar non-linear positive correlations with HER2 copy numbers and HER2/centromere 17 ratios. Moreover, in each HER2-positive IHC/FISH category, higher pCR rates were observed in patients with RNAscope scores of 5 versus 1–4 BC. Patients achieving pCR had BCs with notably higher HER2 RNAscope scores. Multivariate analysis identified HER2 RNAscope 5 as a strong pCR predictor [odds ratio = 10.865, p < 0.001]. The combined impact of multivariate analysis-defined pCR predictors demonstrated that a higher pCR rate was observed in patients with a score of 5 versus a score of 1–4 BCs regardless of the status of hormone receptor and mono-or dual anti-HER2 blockade.
Concusions:
Our results demonstrated that high isHRE (RNAscope score 5) is a strong pCR predictor in patients with HER2-positive BCs receiving NCTT, highlighting the complementary role of isHRE in stratifying HER2 status in tissue. Such stratification is relevant to anti-HER2 therapeutic efficacy, particularly using the cutoff of score 1–4 versus 5.
Linear growth of children with X-linked hypophosphatemia treated with Burosumab: a real-life observational study
Authors: Levy-Shraga Yael , Levi Shelly , Regev Ravit , Gal Shoshana , Brener Avivit , Lebenthal Yael , Gillis David , Strich David , Zung Amnon , Cleper Roxana , Borovitz Yael , Bello Rachel , Tenenbaum Ariel , Zadik Zvi , Davidovits Miriam , Zeitlin Leonid , Tiosano Dov 
Published: June 2023

Abstract
Purpose
To assess the long-term efficacy of burosumab for paediatric patients with X-linked hypophosphatemia, focusing on linear growth.
Methods
This multi-center retrospective study included 35 paediatric patients who began treatment with burosumab between January 2018 and January 2021. We collected clinical data, anthropometric measurements, laboratory results and rickets severity score (RSS), from two years prior to treatment initiation and up to four years after.
Results
Burosumab was initiated at a mean age of 7.5 ± 4.4 years (range 0.6–15.9), with a mean initial dose of 0.8 ± 0.3 mg/kg, which was subsequently increased to 1.1 ± 0.4 mg/kg. The patients were followed for 2.9 ± 1.4 years (range 1–4) after initiating burosumab. Serum phosphorus levels increased from 2.7 ± 0.8 mg/dl at burosumab initiation to 3.4 ± 0.6 mg/dl after three months, and remained stable (p < 0.001). Total reabsorption of phosphorus increased from 82.0 ± 6.8% to 90.1 ± 5.3% after 12 months of treatment (p = 0.041). The RSS improved from 1.7 ± 1.0 at burosumab initiation to 0.5 ± 0.6 and 0.3 ± 0.6 after 12 and 24 months, respectively (p < 0.001). Both height z-score and weight z-score improved from burosumab initiation to the end of the study: from − 2.07 ± 1.05 to -1.72 ± 1.04 (p < 0.001), and from − 0.51 ± 1.12 to -0.11 ± 1.29 (p < 0.001), respectively. Eight children received growth hormone combined with burosumab treatment. Height z-score improved among those who received growth hormone (from − 2.33 ± 1.12 to -1.94 ± 1.24, p = 0.042) and among those who did not (from − 2.01 ± 1.01 to -1.66 ± 1.01, p = 0.001).
Conclusion
Burosumab treatment in a real-life setting improved phosphate homeostasis and rickets severity, and enhanced linear growth.
An Effective, Mechanochemical One-Pot Multi Gram Scale Synthesis of N-Bridged Fused Heterocycles under Solvent- Free Conditions
Authors: Chauhan Swati , Verma Pratibha , Srivast Vandana 
Published: June 2023

Abstract

KI/TBHP mediated C–N and C–S bonds formation of ketones with 2-aminopyridines under grinding condition to give the imidazo[1,2-a]pyridines and imidazo[2,1-b]thiazole under ecologically friendly conditions have been established. The reaction proceeds under solvent-free condition, without using any metal catalyst and displays high yields. This protocol has several notable features including mild reaction condition, base-free, broad functional group tolerance and good reaction yield, avoid the use of expensive and air-sensitive reagents and can be performed under ambient reaction conditions.
Plasmodium falciparum population structure inferred by msp1 amplicon sequencing of parasites collected from febrile patients in Kenya
Authors: Andika Brian , Mobegi Victor , Gathii Kimita , Nyataya Josphat , Maina Naomi , Awinda George , Mutai Beth , Waitumbi John 
Published: June 2023

Abstract
Background
Multiplicity of infection (MOI) is an important measure of Plasmodium falciparum diversity, usually derived from the highly polymorphic genes such as msp1, msp2 and glurp as well as microsatellites. Conventional methods of deriving MOI lack fine resolution needed to discriminate minor clones. This study used amplicon sequencing (AmpliSeq) of Pfmsp1 to measure spatial and temporal genetic diversity of P. falciparum.
Methods
264 P. falciparum positive blood samples collected from areas of differing malaria endemicities between 2010 to 2019 were used. Pfmsp1 gene was amplified and amplicon libraries sequenced on Illumina MiSeq. Sequences were aligned against a reference sequence (NC_004330.2) and clustered to detect fragment length polymorphism and amino acid variations.
Results
Children <5 years had higher parasitemia (median = 23.5+5 SD, p = 0.03) than the >5-14 (= 25.3+5 SD), and those >15 (=25.1+6 SD). Of the alleles detected, 553 (54.5%) were K1, 250 (24.7%) MAD20 and 211 (20.8%) RO33 that grouped into 19 K1 allelic families (108-270 bp), 14 MAD20 (108-216 bp) and one RO33 (153 bp). AmpliSeq revealed nucleotide polymorphisms in alleles that had similar sizes, thus increasing the K1 to 104, 58 for MAD20 and 14 for RO33. By AmpliSeq, the mean MOI was 2.9 (+0.73, 95% CI) for the malaria endemic Lake Victoria region, 2.7 (+ 0.97, 95% CI) for the epidemic prone Kisii Highland and 1.4 (+ 0.19, 95% CI) for the seasonal malaria Semi-Arid region. MOI decreased with age: 2.5 (+ 0.68, 95% CI) for children <5 years, compared to 2.3 (+ 0.65, 95% CI) for ages 5 to 14 and 1.8 (+ 0.71, 95% CI) for those >15. Females had a higher MOI of 2.5 (+ 0.61, 95% CI), compared to males (1.9 (+ 0.49, 95% CI), though not statistically significant. In all regions, the number of alleles increased from the 2014-2015 period, more so in the Lake Victoria and the seasonal transmission arid regions.
Conclusion
Our findings highlight the added advantages of AmpliSeq in allele discrimination. MOI was influenced by age, gender and transmission settings, highlighting an additional level of complexity of P. falciparum population structure.
Gastrointestinal parasites of the wild ungulates (Mammalia: Cetartiodactyla) in the Hemis National Park, Ladakh, India
Authors: Zazay Jigmet , Bhat Bilal A. , Tak Hidayatullah , Lone Arif Nabi 
Published: June 2023

Abstract
Gastrointestinal parasitic infection is known to affect the health and population dynamics of wild ungulates. We evaluated the gastrointestinal parasitic prevalence and intensity of wild ungulates in the Hemis National Park of Ladakh by examining their fresh faecal samples. A total of 327 fresh faecal samples from three wild ungulates, blue sheep Pseudois nayaur (n = 127), Ladakh urial Ovis vignei vignei (n = 110) and Himalayan ibex Ibex siberica (n = 90) were collected between June 2021 and May 2022. The floatation and sedimentation techniques were employed for detection of parasitic eggs/oocysts. Out of 327 samples examined, 165 samples were infected with gastrointestinal parasites making an overall prevalence of (50.45%). During the present study seven parasitic genera were reported. Blue sheep was infected with Eimeria spp. (20.47%), Nematodirus spp. (8.66%), Strongyloides spp. (7.87%) and Moniezia spp. (6.29%). Ladakh urial was infected with Eimeria spp. (17.27%), Trichuris sp. (10.90%), Haemonchus sp. (5.45%) and Moniezia spp. (6.36%). Himalayan ibex was infected with Eimeria spp. (18.88%), Nematodirus spp. (8.88%) and Trichostrongylus spp. (5.55%). Out of 327 faecal samples examined, 36 samples (11%) were infected with mixed parasites. The highest prevalence was recorded in summer (64.42%) and the lowest in winter (33.82%). A significant difference in the parasitic prevalence was observed across seasons in each of the three wild ungulate hosts. However, there was no significant difference in the prevalence of parasites across these hosts.
Microbiome variations among age classes and diets of captive Asian elephants (Elephas maximus) in Thailand using full-length 16S rRNA nanopore sequencing
Authors: Klinsawat Worata , Uthaipaisanwong Pichahpuk , Jenjaroenpun Piroon , Sripib Supaphen , Wongsurawat Thidathip , Kusonmano Kanthida 
Published: June 2023

Abstract
Asian elephants (Elephas maximus) have been known as a national symbol of Thailand and linked to Thai history and culture for centuries. The elephant welfare improvement is one of the major components to achieve sustainable captive management. Microbiome inhabiting digestive tracts have been shown with symbiotic relations to host health. This work provided high-resolution microbiome profiles of 32 captive elephants at a species level by utilizing full-length 16S rRNA gene nanopore sequencing. Eleven common uncultured bacterial species were found across elephants fed with solid food including uncultured bacterium Rikenellaceae RC9 gut group, Kiritimatiellae WCHB1-41, Phascolarctobacterium, Oscillospiraceae NK4A214 group, Christensenellaceae R-7 group, Oribacterium, Oscillospirales UCG-010, Lachnospiraceae, Bacteroidales F082, uncultured rumen Rikenellaceae RC9 gut group, and Lachnospiraceae AC2044 group. We observed microbiome shifts along the age classes of baby (0–2 years), juvenile (2–10 years), and adult (> 10 years). Interestingly, we found distinct microbiome profiles among adult elephants fed with a local palm, Caryota urens, as a supplement. Potential beneficial microbes have been revealed according to the age classes and feed diets. The retrieved microbiome data could be provided as good baseline microbial profiles for monitoring elephant health, and a guideline for dietary selection suitable for each age class.
Multicenter cohort study of the risk factors and mortality of Omicron variant SARS-CoV-2-infected patients with hematologic malignancies in hospitals
Authors: zong xiangping , Zhou Jin , Ma Xiao , Bai Lian , Yan Lingzhi , Geng Hongzhi , Zhang Ying , Li Jiaqi , Yang Qin , Zeng Liangyu , Lu Yutong , Liu Shuangzhu , Li Caixia , Wu Depei 
Published: June 2023

Abstract
Purpose
Evaluating risk factors of mortality and characters in patients with hematologic malignancy (HM) after anti–severe acute respiratory syndrome coronavirus 2 (SARS-CoV2) vaccination of China.
Methods
A total of 104 HMs were included with a median follow-up of 45 days.
Results
The overall mortality rate was 9.6%. In multivariable analyses, 1 or more comorbidities (P = 0.014), lactic dehydrogenase > 300 u/l (P = 0.014), albumin < 35 g/l (P = 0.017) and active malignancy (P = 0.009) were associated with severe and critical COVID-19. Conversely, patients who received 3 vaccinations had a lower possibility of severe infection (P = 0.022). Active malignancy and lactic dehydrogenase > 300 u/l were risk factors associated with higher mortality in multivariable analyses (P = 0.03; P = 0.038, respectively). We also found that the duration of a positive SARS-CoV-2 PCR test and the time to stable pneumonia by chest computed tomography scan in the severe and critical infection subgroups were significantly longer than those in the moderate infection group (P = 0.03 and P = 0.002, respectively).
Conclusions
These findings may contribute to guiding the management of HMs during the pandemic, and emphasizing the importance of starting treatment of aggressive HMs for earlier remission.
Bridging the Gap: A Comprehensive Evaluation of the Government of Tanzania Hospital Management Information System (GoTHOMIS) through Participatory Action Research
Authors: Mambile Cesilia , Mwogosi Augustino 
Published: June 2023

Abstract
This study addresses the implementation and impact of the Government of Tanzania’s Hospital Management Information System (GoTHOMIS) using a participatory action research (PAR) approach. Its purpose is to assess the implementation factors, strengths and weaknesses of GoTHOMIS on healthcare services in Tanzania. The research employs a PAR methodology involving stakeholders from healthcare facilities, administrators, and I.T. experts. Data collection methods include document evaluation and interviews with key informants. The findings highlight significant areas for improvement in the implementation process, characterized by a top-down approach and a lack of user participation, hindering the system’s successful adoption. Inadequate infrastructure, including network connectivity and outdated hardware, posed further challenges. Compatibility issues with peripheral devices and usability problems were also identified. Despite the challenges, GoTHOMIS demonstrated strengths such as improved accessibility, integration with the MTUHA reporting system, and automation of clinical tasks—weaknesses, including some system components, were only partially automated, which caused inefficiencies in the delivery of healthcare services and failure in integrating with other existing healthcare systems and data sources. Limitations of the study include a focus on selected healthcare facilities, limiting generalizability, and reliance on self-reported data, which may introduce biases. The study’s originality lies in applying a PAR approach to assess GoTHOMIS, providing valuable insights into implementation challenges and potential improvements.
Coping strategies mediate the relationship between fear of cancer recurrence and quality of life in postoperative patients with prostate cancer: a multicentre survey
Authors: Yu Chen , Jingzhen Zhu , Luqiang Zhou , Xiaojuan Yuan , Ji Zheng 
Published: June 2023

Abstract
Purpose
The aim of the present study was to investigate the relationships between fear of cancer recurrence and quality of life in patients with prostate cancer. A model based on Lazarus’ and Folkman’s stress and coping theory tested the specific hypothesis: fear of cancer recurrence has a direct and indirect effect on quality of life mediated by coping strategies.
Methods
The study used a cross-sectional and correlational design. Patients (n = 305) with postoperative prostate cancer completed three questionnaires and provided background data in 2023, and a mediator model was tested using the PROCESS macro for SPSS.
Results
The test of the conceptual model confirmed the hypothesis. There was a significant direct and indirect effect of fear of cancer recurrence on quality of life through the mediators.
Conclusions
The coping strategies of face and yield are the most important mediating factors between fear of cancer recurrence and QoL in patients with chronic illness, which supports Lazarus’ and Folkman’s stress and coping theory.
Endogenous Market Coverage with Quality Dependent Production Costs
Authors: Pires Cesaltina Pacheco , Jorge Sílvia Ferreira , Catalão-Lopes Margarida , Pinho Joana , Garcês Pedro , Alventosa Adriana 
Published: June 2023

Abstract
This paper endogenizes market coverage in a vertically differentiated duopoly with quality dependent marginal production costs, where firms first choose qualities and then prices. When the lowest quality valuation is low, a partial coverage duopoly emerges. For slightly higher lowest quality valuation, a full coverage duopoly with a corner solution holds. For even higher lowest quality valuation, there are multiple equilibria. For highest lowest quality valuation, there is an interior full coverage duopoly. Interestingly, product differentiation increases with quality valuation heterogeneity, given a market configuration, but may decrease when accounting for an endogenous market coverage.
JEL classification: L13; L15; D43
Contact toxicity, antifeedant activity and oviposition preference of osthole against agricultural pests
Authors: Dong Fang , Chen Xin , Xingyuan Men , Li Zhuo , Kong Yujun , Yuan Yiyang , Ge Feng 
Published: June 2023

Abstract
Osthole, the dominant bioactive constituent in Cnidium monnieri, has been shown to exhibit acute insecticidal activities. However, its detailed toxicity, antifeedant and oviposition preference effects against agricultural pests has not been fully understood, which has greatly hindered its practical applications. This study is designed to investigate the contact toxicity, antifeedant activity and oviposition preference of osthole against three agricultural pests (Tetranychus urticae, Myzus persicae and Bactrocera dorsalis) to evaluate its potential agricultural applications. Our results showed that Cnidium monnieri (L.) Cusson (CMC) have a high osthole content of 11.4 mg/g. Osthole exhibited a comparable level of acute toxicity against T. urticae to four other coumarins found in CMC. Osthole demonstrated significant insecticidal activity against first instar nymphs and adults of T. urticae and M. persicae in a dose-dependent manner, but not against B. dorsalis adults. Osthole exposure reduced the fecundity and prolonged the developmental time of T. urticae and M. persicae. Leaf choice bioassays revealed potent antifeedant activity in T. urticae. Furthermore, female B. dorsalis showed a distinct preference for laying eggs in mango juice with 0.02 mg/mL osthole at 48 hours, a preference which persisted at 96 hours. These results provide valuable insights into the toxicity, repellent activity, and attractant activity of osthole, thereby contributing to its expanded use in pest control.
Integrating UAV Derived Vegetation and Texture Indices for Estimation of Leaf Nitrogen Concentration in Drip-Irrigated Cotton under Reduced Nitrogen Treatment and Different Plant Densities
Authors: Li Minghua , Liu Yang , Lu Xi , Jiang Jiale , Ma Xuehua , Wen Ming , Ma Fuyu 
Published: June 2023

Abstract
Background
Accurate assessment of nitrogen (N) status is important for N management and yield improvement. The N status in plant was affected by plant densities and N application rates, while the methods of assessing the N status in drip-irrigated cotton under reduced nitrogen treatment and different plant densities are lacking.
Methods
This study was conducted with four different N treatments (195.5, 299, 402.5, and 506 kg N ha− 1) and three sowing densities (6.9×104, 13.8×104, and 24×104 plants ha− 1) by using a low-cost Unmanned Aerial Vehicle (UAV) system to acquire RGB imagery at 10 m flight altitude at cotton main growth stages. We evaluated the performance of different ground resolutions (1.3-, 2.6-, 5.2-,10.4-, 20.8-, 41.6-, 83.2-, and 166.4-cm-ground-resolution) image textures, vegetation indices (VIs), and their combination for leaf N concentrations (LNC) estimation with four regression methods (stepwise multiple linear regression, SMLR; support vector regression, SVR; extreme learning machine, ELM; random forest, RF).
Results
The results showed that the combination of VIs and texture maintained higher estimation accuracy than using VIs or textures alone. Specifically, the RF regression models had the higher accuracy and stability than SMLR and other two machine learning algorithms. The best accuracy (R2 = 0.87, RMSE = 3.14g kg− 1, rRMSE = 7.00%) was obtained when RF was applied in combination with VIs and texture.
Conclusion
The combination of VIs and textures from UAV images using RF could improve the estimation accuracy of drip-irrigated cotton LNC and may have the potential contribution in rapid and non-destructive nutrition monitoring and diagnosis of other crops or other growth parameters.