bluuebunny/crossref_metadata_2025_split huggingface dataset:
Host E3 ligase Hrd1 ubiquitinates and degrades H protein of canine distemper virus to inhibit viral replication
Authors: Wang Wenjie , Bi Zhenwei , Song Suquan 
Published: April 2023

AbstractCanine distemper (CD) is a highly contagious and an acutely febrile disease caused by canine distemper virus (CDV), which greatly threatens the dog and fur industry in many countries. Endoplasmic reticulum (ER)-associated degradation (ERAD) is a protein quality control system for the degradation of misfolded proteins in the ER. In this study, a proteomic approach was performed, and results found the E3 ubiquitin ligase 3-hydroxy-3-methylglutaryl reductase degradation protein 1 (Hrd1), which is involved in ERAD, as one of the CDV H-interacting proteins. The interaction of Hrd1 with CDV H protein was further identified by Co-IP assay and confocal microscopy. Hrd1 degraded the CDV H protein via the proteasome pathway dependent on its E3 ubiquitin ligase activity. Hrd1 catalyzed the K63-linked polyubiquitination of CDV H protein at lysine residue 115 (K115). Hrd1 also exhibited a significant inhibitory effect on CDV replication. Together, the data demonstrate that the E3 ligase Hrd1 mediates the ubiquitination of CDV H protein for degradation via the proteasome pathway and inhibits CDV replication. Thus, targeting Hrd1 may represent a novel prevention and control strategy for CDV infection.
Molecular mechanisms of neurite regeneration and repair: insights from C. elegans and Drosophila
Authors: Liu Xiaofan , Zhao Yuqing , Zou Wei 
Published: April 2023

AbstractThe difficulties of injured and degenerated neurons to regenerate neurites and regain functions are more significant than in other body tissues, making neurodegenerative and related diseases hard to cure. Uncovering the secrets of neural regeneration and how this process may be inhibited after injury will provide insights into novel management and potential treatments for these diseases. Caenorhabditis elegans and Drosophila melanogaster are two of the most widely used and well-established model organisms endowed with advantages in genetic manipulation and live imaging to explore this fundamental question about neural regeneration. Here, we review the classical models and techniques, and the involvement and cooperation of subcellular structures during neurite regeneration using these two organisms. Finally, we list several important open questions that we look forward to inspiring future research.
A new class of computationally efficient algorithms for solving fixed-point problems and variational inequalities in real Hilbert spaces
Authors: Kumam Wiyada , Rehman Habib ur , Kumam Poom 
Published: April 2023

AbstractA family of inertial extragradient-type algorithms is proposed for solving convex pseudomonotone variational inequality with fixed-point problems, where the involved mapping for the fixed point is a ρ-demicontractive mapping. Under standard hypotheses, the generated iterative sequence achieves strong convergence to the common solution of the variational inequality and fixed-point problem. Some special cases and sufficient conditions that guarantee the validity of the hypotheses of the convergence statements are also discussed. Numerical applications in detail illustrate the theoretical results and comparison with existing methods.
An ensemble method for estimating the number of clusters in a big data set using multiple random samples
Authors: Mahmud Mohammad Sultan , Huang Joshua Zhexue , Ruby Rukhsana , Wu Kaishun 
Published: April 2023

AbstractClustering a big dataset without knowing the number of clusters presents a big challenge to many existing clustering algorithms. In this paper, we propose a Random Sample Partition-based Centers Ensemble (RSPCE) algorithm to identify the number of clusters in a big dataset. In this algorithm, a set of disjoint random samples is selected from the big dataset, and the I-niceDP algorithm is used to identify the number of clusters and initial centers in each sample. Subsequently, a cluster ball model is proposed to merge two clusters in the random samples that are likely sampled from the same cluster in the big dataset. Finally, based on the ball model, the RSPCE ensemble method is used to ensemble the results of all samples into the final result as a set of initial cluster centers in the big dataset. Intensive experiments were conducted on both synthetic and real datasets to validate the feasibility and effectiveness of the proposed RSPCE algorithm. The experimental results show that the ensemble result from multiple random samples is a reliable approximation of the actual number of clusters, and the RSPCE algorithm is scalable to big data.
Electrical response of fungi to changing moisture content
Authors: Phillips Neil , Gandia Antoni , Adamatzky Andrew 
Published: April 2023

AbstractMycelium-bound composites are potential alternatives to conventional materials for a variety of applications, including thermal and acoustic building panels and product packaging. If the reactions of live mycelium to environmental conditions and stimuli are taken into account, it is possible to create functioning fungal materials. Thus, active building components, sensory wearables, etc. might be created. This research describes the electrical sensitivity of fungus to changes in the moisture content of a mycelium-bound composite. Trains of electrical spikes initiate spontaneously in fresh mycelium-bound composites with a moisture content between $$\sim$$
∼
95% and $$\sim$$
∼
65%, and between $$\sim$$
∼
15% and $$\sim$$
∼
5% when partially dried. When the surfaces of mycelium-bound composites were partially or totally encased with an impermeable layer, increased electrical activity was observed. In fresh mycelium-bound composites, electrical spikes were seen both spontaneously and when induced by water droplets on the surface. Also explored is the link between electrical activity and electrode depth. Future designs of smart buildings, wearables, fungi-based sensors, and unconventional computer systems may benefit from fungi configurations and biofabrication flexibility.
Belowground microbiota analysis indicates that Fusarium spp. exacerbate grapevine trunk disease
Authors: Li Yonghua , Li Xinghong , Zhang Wei , Zhang Jiao , Wang Hui , Peng Junbo , Wang Xuncheng , Yan Jiye 
Published: April 2023

Abstract
Background
Grapevine trunk diseases (GTDs) are disease complexes that are major threats to viticulture in most grapevine growing regions. The microbiomes colonizing plant belowground components form complex associations with plants, play important roles in promoting plant productivity and health in natural environments, and may be related to GTD development. To investigate associations between belowground fungal communities and GTD symptomatic or asymptomatic grapevines, fungal communities associated with three soil–plant compartments (bulk soils, rhizospheres, and roots) were characterized by ITS high-throughput amplicon sequencing across two years.

Results
The fungal community diversity and composition differs according to the soil–plant compartment type (PERMANOVA, p < 0.001, 12.04% of variation explained) and sampling year (PERMANOVA, p < 0.001, 8.83%), whereas GTD symptomatology exhibited a weaker, but still significant association (PERMANOVA, p < 0.001, 1.29%). The effects of the latter were particularly prominent in root and rhizosphere community comparisons. Many GTD-associated pathogens were detected, but their relative abundances were not correlated (or were negatively correlated) to symptomatology. Fusarium spp., were enriched in symptomatic roots and rhizospheres compared to asymptomatic counterparts, suggesting that their abundances were positively correlated with symptomatic vines. Inoculation tests revealed that Fusarium isolates, similar to Dactylonectria macrodidyma, a pathogen associated with black foot disease, caused dark brown necrotic spots on stems in addition to root rot, which blackened lateral roots. Disease indices were higher with co-inoculation than single inoculation with a Fusarium isolate or D. macrodidyma, suggesting that Fusarium spp. can exacerbate disease severity when inoculated with other known GTD-associated pathogens.

Conclusions
The belowground fungal microbiota of grapevines varied from soil–plant compartments, the years and whether showed GTD symptoms. The GTDs symptoms were related to the enrichment of Fusarium spp. rather than the relative abundances of GTD pathogens. These results demonstrate the effects of fungal microbiota of roots and rhizospheres on GTDs, while providing new insights into opportunistic pathogenesis of GTDs and potential control practices.
The use of films in the teaching of English as a foreign language: a systematic literature review
Authors: Sánchez-Auñón Estefanía , Férez-Mora Pedro Antonio , Monroy-Hernández Fuensanta 
Published: April 2023

AbstractBackgroundEspecially over the last decades, the use of cinema in the English as a foreign language (EFL) class has been gaining momentum. Although this interest has resulted in a complex body of research, no review to date had aimed to systematically map out (i) the pedagogical guidelines available for English teachers to implement films in class; (ii) the perceptions of EFL teachers and learners on the educational use of films; and (iii) the impact of cinema-based EFL on students’ learning, pertinent aspects as they contribute to deactivate instructors’ reported reluctance to use films as a proper teaching material. Thus, a systematic literature review has been conducted along the three previously-mentioned research questions with the aim of highlighting in a comprehensive manner the robust pedagogical value of cinema in EFL contexts.MethodsA systematic search was carried out in Web of Science, ERIC, MLA International Bibliography, Education Database, and Scopus, which were last consulted on 2 December, 2022. Inclusion and exclusion criteria were applied, which covered journal papers written in English, peer-reviewed, published in open-access, and focusing on the exploitation of films for the teaching of English as a foreign language. Following the search and selection stages, a coding scheme was established, and the authors conducted a thematically based qualitative analysis to answer the research questions. Reliability was tested to check the agreement rate among the researchers.FindingsOf the 416 sources found, 44 were eligible. Twenty-five percent of the selected references consist of theoretical or practical methodological orientations for the implementation of films in the EFL class, 16% of the sources explore EFL teachers’ and students’ perceptions on the educational use of films, and the remaining 59% of the references tap into the impact of cinema-based EFL on students’ learning. The results revealed that more informed guidelines on the exploitation of this resource are needed, that both instructors and teachers have a great attitude towards this method, and that films provide EFL learners with linguistic, intercultural, and motivational benefits.InterpretationOn a general note, scholars have adopted a narrow focus when exploring cinema-based English teaching since they only address some of its benefits and they have mostly concentrated on the University educational context. Specific research gaps are highlighted in relation to each research question, and avenues for further research are proposed. Finally, pedagogical implications for the educational use of cinema in the English class are provided.
Transdermal drug delivery system of lidocaine hydrochloride based on dissolving gelatin/sodium carboxymethylcellulose microneedles
Authors: Bahmani Shabnam , Khajavi Ramin , Ehsani Morteza , Rahimi Mohammad Karim , Kalaee Mohammad Reza 
Published: April 2023

AbstractIn this study, it was aimed to introduce a transdermal drug delivery system with dissolving microneedles (DMNs) based on gelatin (GEL) and sodium carboxymethyl cellulose (NaCMC) for lidocaine hydrochloride (LidoHCl) delivery. Different ratios of GEL and NaCMC were mixed, loaded with an active agent of LidoHCl, and treated with glutaraldehyde (GTA) as a crosslinker agent. Prepared hydrogels were cast into a silicon mold. Hereby, microneedles (MNs) with 500 µm height, 35° needle angle, 40-µm tip radius, and 960-µm tip-to-tip distance were fabricated. Samples containing LidoHCl 40%, GEL/NaCMC 5:1 (wt/wt), and polymer/GTA ratio 3.1 (wt/wt) showed the highest drug release ability (t < 10 min) with proper mechanical properties in comparison with other samples. Due to the drug release in a short time (fewer than 10 min), this drug delivery system can be used for rapid local anesthesia for pain relief as well as before minor skin surgeries.
Graphical Abstract
Quantitative assessment of PSMA PET response to therapy in castration-sensitive prostate cancer using an automated imaging platform for disease identification and measurement
Authors: Duriseti Sai , Berenji Gholam , Tsai Sonny , Rettig Matthew , Nickols Nicholas G. 
Published: April 2023

Abstract
Rationale
Prostate cancer treatment response may be automatically quantified using a molecular imaging analysis platform targeting prostate-specific membrane antigen (PSMA).

Methods
A retrospective analysis of patients with castration-sensitive prostate cancer who underwent PSMA-targeted molecular imaging prior to and 3 months or more after treatment was conducted. Disease burden was analyzed with aPROMISE, an artificial intelligence imaging platform that automatically quantifies PSMA-positive lesions. The calculated PSMA scores for prostate/bed, nodal, and osseous disease sites were compared with prostate-specific antigen (PSA) values.

Results
Of 30 eligible patients, the median decline in prostate/bed, nodal, and osseous disease PSMA scores were 100% (range 52–100%), 100% (range − 87–100%), and 100% (range − 21–100%), respectively. PSMA score decline was significantly associated with PSA decline.

Conclusion
Changes in aPROMISE PSMA scores are associated with changes in PSA and may quantify treatment response.
Perceived benefits of cochlear implants by parents: expectations, decision-making process, and barriers to care
Authors: Ibrahim Ateya Megahed , El-Gilany Abdel-Hady , Mohamed Eman Wardany Abdelaal , Farrag Nesrine Saad 
Published: April 2023

Abstract
Background
Identifying predictive factors of the cochlear implant outcomes in pediatric patients is critical in guiding tailored rehabilitation programs. The study aimed to assess cochlear implant outcomes, identify predictors, and highlight decision-making factors and barriers to quality care.

Methods
This cross-sectional study included parents of children who received unilateral cochlear implants for bilateral severe- to- deep sensorineural hearing loss. Inclusion criteria were age ≤ 5 years and intelligence quotient (IQ) Scores ≥ 85, A pre-designed structured questionnaire was used to collect data from parents/guardians of the children attending follow-up. The Arabic validated Glasgow Children Benefit Inventory score was used to assess the health-related quality of life (QOL) after intervention.

Results
The quality of life (QOL) score (outcome) after surgery was positive in all cases. Multivariate analysis showed that the site of operation (Bahtim hospital and Ain Shams Hospital [AOR(95% confidence interval CI), 5.7 (1.4–23), 5 (1.4–17.9), p = 0.015, 0.013, respectively]), education of the father (university/postgraduate [AOR (95% CI): 5 (1.4–17.9), p = 0.013]), the parents’ expectation that their child would be able to participate in regular classroom activities [AOR (95% CI): 8.9 (3.7–21.3), p < 0.001], and history of Attention deficit/hyperactivity disorder (ADHD), perinatal hypoxia, and low birth weight [AOR (95% CI): 2.5 (1.2–5.1), 3.7 (1.7–8.1), 4.7 (2.1–10.5), p = 0.013, 0.001, ≤ 0.001, respectively] are significant independent predictors of good outcome.

Conclusion
All parents expressed a positive change in their child’s QOL. Almost all parents of children with cochlear implants face many barriers in obtaining quality healthcare services for their children. Parents, especially those with lower schooling, should receive good counselling to increase their confidence in their children’s capabilities and maximize benefits of regular follow-up. Improving the quality of healthcare centers is recommended.
Effect of cottonseed milk on growth performance, hematological and semen characteristics in male Wistar albino rats
Authors: Subramani Thirukkumar , Ganapathyswamy Hemalatha , Sampathrajan Vellaikumar , David Raj C. 
Published: April 2023

AbstractVarious plant based milk extract is appropriate for human nutrition among which cottonseed is one of the potential crops with the advantages of stable milk emulsion, improved nutrient profile with affordable waste utilization. Although cottonseed milk is a popular indigenous beverage, it has not been exploited worldwide for regular consumption owing to the presence of gossypol. The gossypol toxicity and associated gossypol-iron complex formation in the intestine lead to changes in hematological characteristics and alternation of sperm motility in monogastric animals. Male Wistar albino rats weighing 60 to 70 g were divided into four groups of five animals each. The group fed the standard diet (STD) served as control, and the experimental groups included the group (i) rats fed cottonseed diet (CSD) supplemented at 10% of cottonseed level (ii) rats fed conventional aqueous extracted cottonseed milk (CCM) diet (CAD) and (iii) rats fed enzymatic assisted aqueous extracted cottonseed milk (ECM) diet (EAD). The CAD and EAD feed was administrated @ 1 ml/100 g of animal body weight /day for the study period of 45 days among the experimental groups and control group. A significant difference in weight gain of the experimental rats was noticed between the CCM and ECM cottonseed milk extracts fed experimental groups compared with the standard and cottonseed fed groups. The rats fed with CSD, CCM and ECM diet exhibited higher white blood cell counts, also reducing the red blood cells count, hemoglobin hematocrit and platelet in the group compared with STD. No significant difference in semen motility characteristics was noticed among the CSD, CCM and ECM fed groups. In conclusion, the intake of less than the permissible level of gossypol from selected cottonseed and its aqueous extracted milk samples has influenced the hematological parameters and whereas an improved effect was shown in semen characteristics.
Graphical Abstract
Integrity monitoring scheme for single-epoch GNSS PPP-RTK positioning
Authors: Zhang Wenhao , Wang Jinling 
Published: April 2023

AbstractIntegrity monitoring for precise point positioning is critical for safety-related applications. With the increasing demands of high-accuracy autonomous navigation for unmanned ground and aerial vehicles, the integrity monitoring method of high-precision positioning has become an essential requirement. While high precision Global Navigation Satellite Systems (GNSS) positioning is widely used in such applications, there are still many difficulties in the integrity monitoring method for the multi-frequency multi-GNSS undifferenced and uncombined Precise Point Positioning (PPP). The main difficulties are caused by using the measurements of multiple epochs in PPP. Based on the baseline Multiple Hypothesis Solution Separation (MHSS) Advanced Receiver Autonomous Integrity Monitoring (ARAIM) algorithm, this paper discusses the feasibility of the pseudorange-based baseline ARAIM method on the single-epoch PPP based on Real-Time Kinematic (RTK) networks (PPP-RTK) framework to overcome these difficulties. In addition, a new scheme is proposed to transfer the conventional PPP process into the single-epoch PPP-RTK framework. The simulation results using the proposed model are analyzed in this study. The Protection Levels (PLs) estimated by PPP Wide-lane Ambiguity Resolution (PPP-WAR) model with regional corrections can reach the meter level and the PLs estimated by PPP Ambiguity Resolution (PPP-AR) and PPP-RTK models are usually the sub-meter level. Given a horizontal Alert Limit (AL) of 1.5 m, the global coverage of availability above 99.9% for PPP-WAR, PPP-AR, and PPP-RTK can reach 92.6%, 99.4%, and 99.7% respectively. The results using real kinematic data also show that tight PLs can be achieved when the observation conditions are good.
A content analysis of alignment messages to the Next Generation Science Standards
Authors: Tanas Jamie , Fulmer Gavin 
Published: April 2023

AbstractTeachers are a critical component to standards-based reform systems, which require that reforms conceived at the national level pass through several layers of the educational system before impacting learning in the classroom. The Next Generation Science Standards (NGSS) are an example of this type of reform and pose significant challenges for alignment between levels given their three-dimensional nature alongside inclusion of ambitious and novel reform ideas. To examine translation of NGSS reforms across levels, we provide a content analysis of alignment messages conveyed to teachers through practitioner literature. Analysis indicates some coherence with national messaging around alignment to performance expectations and science and engineering practices. Additionally, alignment to broader reform ideas like engaging in science practices, integration, engineering, and focus on phenomena were represented to teachers. However, qualitative analysis of these representations indicate that reforms are often superficially portrayed, variably defined, or missing altogether. Findings indicate that teachers receive numerous messages regarding what it means to align to the NGSS and few elaborations on how to operationalize reforms. Our work suggests a need for intentional consideration of how to design representations for practitioners that consider teacher sensemaking around novel reforms. Additionally, we see a need for further development of coherence among the research community regarding alignment to the NGSS and agreement on definition of key reform ideas. Future work should consider how teachers use and understand these representations as they enact the NGSS in their local contexts.
Early enteral versus early parenteral nutrition in critically ill patients with respiratory distress: a case–control study
Authors: El Meligy Basant Salah , El-sherbini Seham Awad , Soliman Mohamed Mosaad , abd El-Ghany Heba Mohamad , Ahmed Elshimaa Salah 
Published: April 2023

Abstract
Background
Nutritional support is essential as enteral or parenteral nutrition to reduce catabolism, to lower the complications rate, and to improve outcomes in critically ill patients.

Results
The median, range age of the cohort was (median 10, range 6–18.8 months). One-hundred thirteen (62.8%) were males, and 67 (37.2%) were females. The higher frequency of sepsis, ventilator-acquired pneumonia (VAP), and mortality founded in the group received PN. Frequency of sepsis was 15 (16.7%), VAP was 5 (5.6%), and the mortality rate was 11.1% in EN group, while frequency of sepsis was 37 (41.1%), VAP was 23 (25.6%), and the mortality rate was 27.8% in PN group (P = 0.001, 0.001, 0.01, respectively). Median of weight gain on the EN group was 0.17 kg at 2nd week which was more than those in PN group (P = 0.001). The mean ± SD time for reaching the caloric target for those receiving early EN was 4.0 ± 1.9 days which is earlier than that of PN group (6.2 ± 1.7 days) (P = 0.001). There is no significant difference between both groups as regard pediatric intensive care unit (PICU) stay length and mechanical ventilation stay length.

Conclusion
Early EN remains the preferred route for nutrient delivery as the PN route was accompanied by a lot of complication such as sepsis, VAP, and high mortality rate.