bluuebunny/crossref_metadata_2025_split huggingface dataset:
Cocirculation of A(H3N2) and B/Victoria increased morbidity in hospitalized patients in the 2019–2020 A(H1N1)pdm09 predominant influenza season in Israel
Authors: Jurkowicz Menucha , Nemet Ital , Atari Nofar , Fratty Ilana S. , Kliker Limor , Sherbany Hilda , Keller Nathan , Leibovitz Eugene , Mendelson Ella , Mandelboim Michal , Stein Michal 
Published: January 2023

AbstractCommunity surveillance found the 2019–2020 A(H1N1)pdm09 predominant influenza season in Israel to be a high‐intensity season with an early and steep morbidity peak. To further characterize disease severity in the 2019–2020 season, we analyzed a cohort of hospitalized patients with laboratory‐confirmed influenza from this season (n = 636). Quantitative polymerase chain reaction was performed on clinical samples to detect the presence of influenza. Demographic, clinical, and laboratory data were retrieved via electronic health records and MDClone. Electronic health records were accessed to obtain data on intensive care unit patients, missing data and for data verification purposes. Univariate analysis was performed to compare demographic, comorbidity, and clinical characteristics across the three influenza strains. The A(H1N1)pdm09 predominant 2019–2020 influenza season in Israel was characterized by an early and steep morbidity peak, vaccine delays and shortages, and with the A(H3N2) and B/Victoria strains disproportionately targeting children and young adults, most probably due to reduced immunity to these strains. A greater proportion of children <5 years infected with A(H3N2) and B/Victoria developed severe influenza compared with those infected with A(H1N1)pdm09. Our study emphasizes the vulnerability of infants and young children in the face of rapidly evolving influenza strains and underscores the importance of influenza prevention measures in this population.
A hepatitis B virus core antigen‐based virus‐like particle vaccine expressing SARS‐CoV‐2 B and T cell epitopes induces epitope‐specific humoral and cell‐mediated immune responses but confers limited protection against SARS‐CoV‐2 infection
Authors: Hassebroek Anna M. , Sooryanarain Harini , Heffron Connie L. , Hawks Seth A. , LeRoith Tanya , Cecere Thomas E. , Stone William B. , Walter Debra , Mahsoub Hassan M. , Wang Bo , Tian Debin , Ivester Hannah M. , Allen Irving C. , Auguste Albert J. , Duggal Nisha K. , Zhang Chenming , Meng Xiang‐Jin 
Published: January 2023

AbstractThe hepatitis B virus core antigen (HBcAg) tolerates insertion of foreign epitopes and maintains its ability to self‐assemble into virus‐like particles (VLPs). We constructed a ∆HBcAg‐based VLP vaccine expressing three predicted severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2) B and T cell epitopes and determined its immunogenicity and protective efficacy. The recombinant ∆HBcAg‐SARS‐CoV‐2 protein was expressed in Escherichia coli, purified, and shown to form VLPs. K18‐hACE2 transgenic C57BL/6 mice were immunized intramuscularly with ∆HBcAg VLP control (n = 15) or ∆HBcAg‐SARS‐CoV‐2 VLP vaccine (n = 15). One week after the 2nd booster and before virus challenge, five ∆HBcAg‐SARS‐CoV‐2 vaccinated mice were euthanized to evaluate epitope‐specific immune responses. There is a statistically significant increase in epitope‐specific Immunoglobulin G (IgG) response, and statistically higher interleukin 6 (IL‐6) and monocyte chemoattractant protein‐1 (MCP‐1) expression levels in ∆HBcAg‐SARS‐CoV‐2 VLP‐vaccinated mice compared to ∆HBcAg VLP controls. While not statistically significant, the ∆HBcAg‐SARS‐CoV‐2 VLP mice had numerically more memory CD8+ T‐cells, and 3/5 mice also had numerically higher levels of interferon gamma (IFN‐γ) and tumor necrosis factor (TNF). After challenge with SARS‐CoV‐2, ∆HBcAg‐SARS‐CoV‐2 immunized mice had numerically lower viral RNA loads in the lung, and slightly higher survival, but the differences are not statistically significant. These results indicate that the ∆HBcAg‐SARS‐CoV‐2 VLP vaccine elicits epitope‐specific humoral and cell‐mediated immune responses but they were insufficient against SARS‐CoV‐2 infection.
Effects of trauma‐focused rumination among trauma‐exposed individuals with and without posttraumatic stress disorder: An experiment
Authors: Wisco Blair E. , Vrshek‐Schallhorn Suzanne , May Casey L. , Campbell Allison A. , Nomamiukor Faith O. , Pugach Cameron P. 
Published: January 2023

AbstractRumination, or thinking repetitively about one's distress, is a risk factor for posttraumatic stress disorder (PTSD). Current theories suggest that rumination contributes to PTSD symptoms directly, by increasing negative reactions to trauma cues (i.e., symptom exacerbation), or represents a form of cognitive avoidance, if verbal ruminations are less distressing than trauma imagery. The goal of this study was to test the symptom exacerbation and cognitive avoidance accounts of trauma‐focused rumination. We recruited 135 trauma‐exposed participants (n = 60 diagnosed with PTSD) and randomly assigned them to ruminate about their trauma, distract themselves, or engage in trauma imagery. For individuals with and without PTSD, rumination led to larger increases in subjective distress (i.e., negative affect, fear, sadness, subjective arousal, valence) than distraction, ηp2s = .04–.13, but there were no differences between rumination and imagery ηp2s = .001–.02. We found no evidence that rumination or imagery elicited physiological arousal, ds = 0.01–0.19, but did find that distraction reduced general physiological arousal, as measured by heart rate, relative to baseline, d = 0.84, which may be due to increases in parasympathetic nervous system activity (i.e., respiratory sinus arrhythmia), d = 0.33. These findings offer no support for the avoidant function of rumination in PTSD. Instead, the findings were consistent with symptom exacerbation, indicating that rumination leads directly to emotional reactivity to trauma reminders and may be a fruitful target in PTSD intervention.
Drivers of survival of translocated tortoises
Authors: Mack Jeremy S. , Berry Kristin H. 
Published: January 2023

AbstractTranslocation of animals, especially for threatened and endangered species, is a currently popular but very challenging activity. We translocated 158 adult Agassiz's desert tortoises (Gopherus agassizii), a threatened species, from the National Training Center, Fort Irwin, in the central Mojave Desert in California, USA, to 4 plots as part of a long‐distance, hard‐release, mitigation‐driven translocation to prevent deaths from planned military maneuvers. We monitored demographic and behavioral variables of tortoises fitted with radio‐transmitters from 2008 to 2018. By the end of the project, 17.72% of tortoises were alive, 65.82% were dead, 15.19% were missing, and 1.27% were removed from the study because they returned to Fort Irwin. Mortality was high during the first 3 years: >50% of the released animals died, primarily from predation. Thereafter, mortality declined but remained high. After 10.5 years, survival was highest, 37.50% (15/40), on the plot closest to original home sites, whereas from 2.56% to 23.68% remained alive on the other 3 release plots. Surviving tortoises settled early, repeatedly using locations where they constructed burrows, compared with tortoises that died or disappeared. Models of behavioral and other variables indicated that numbers of repeatedly used locations (burrows) were a driver of survival throughout the study, although plot location, size and sex of tortoises, and distance traveled were contributors, especially during early years. Because >50% mortality occurred, we considered this translocation unsuccessful. The study area appeared to be an ecological sink with historical and current anthropogenic uses contributing to habitat degradation and a decline in both the resident and released tortoises. Our findings will benefit design and selection of future translocation areas.
On the logistic equation for the fractional p‐Laplacian
Authors: Iannizzotto Antonio , Mosconi Sunra , Papageorgiou Nikolaos S. 
Published: January 2023

AbstractWe consider a Dirichlet problem for a nonlinear, nonlocal equation driven by the degenerate fractional p‐Laplacian, with a logistic‐type reaction depending on a positive parameter. In the subdiffusive and equidiffusive cases, we prove existence and uniqueness of the positive solution when the parameter lies in convenient intervals. In the superdiffusive case, we establish a bifurcation result. A new strong comparison result, of independent interest, plays a crucial role in the proof of such bifurcation result.
Clinical features and molecular characterization of Chinese patients with FKBP10 variants
Authors: Tan Zhijia , Shek Hiu Tung , Chen Peikai , Dong Zhongxin , Zhou Yapeng , Yin Shijie , Qiu Anmei , Dong Lina , Gao Bo , To Michael Kai Tsun 
Published: January 2023

AbstractBackgroundOsteogenesis imperfecta (OI) is a group of rare skeletal dysplasia. Long bone deformity and scoliosis are often associated with progressively deforming types of OI. FKBP65 (encoded by FKBP10, OMIM *607063) plays a crucial role in the processing of type I procollagen. Autosomal recessive variants in FKBP10 result in type XI osteogenesis imperfecta.MethodsPatients diagnosed with OI were recruited for a genetic test. RT‐PCR and Sanger sequencing were applied to confirm the splicing defect in FKBP10 mRNA with the splice‐site variant. The bone structure was characterized by Goldner's trichrome staining. Bioinformatic analyses of bulk RNA sequencing data were performed to examine the effect of the FKBP10 variant on gene expression.ResultsHere we reported three children from a consanguineous family harboured a homozygous splice‐site variant (c.918‐3C > G) in FKBP10 intron and developed long bone deformity and early onset of scoliosis. We also observed frequent long bone fractures and spinal deformity in another 3 OI patients with different FKBP10 variants. The homozygous splicing variant identified in the fifth intron of FKBP10 (c.918‐3C > G) led to abnormal RNA processing and loss of FKBP65 protein and consequently resulted in aberrant collagen alignment and porous bone morphology. Analysis of transcriptomic data indicated that genes involved in protein processing and osteoblast differentiation were significantly affected in the patient‐derived osteoblasts.ConclusionOur study characterized the clinical features of OI patients with FKBP10 variants and revealed the pathogenesis of the c.918‐3C > G variant. The molecular analyses helped to gain insight into the deleterious effects of FKBP10 variants on collagen processing and osteoblast differentiation.
Exploring cooperative molecular contacts using a PostgreSQL database system
Authors: Briand Mael A. , Dreano Loïc , Legehar Ashenafi , Grazhdankin Evgeni , Ghemtio Léo , Xhaard Henri 
Published: January 2023

AbstractCooperative molecular contacts play an important role in protein structure and ligand binding. Here, we constructed a PostgreSQL database that stores structural information in the form of atomic environments and allows flexible mining of molecular contacts. Taking the Ser‐His‐Asp/Glu catalytic triad as a first test case, we demonstrate that the presence of a carboxylate oxygen atom in the vicinity of a His is associated with shorter Ser‐OH..N‐His bond in the PDB30 subset. We prospectively mine catalytic triads in unannotated proteins, suggesting catalytic functions for unannotated proteins. As a second test case, we demonstrate that this database system can include ligand atoms, represented by Sybyl atom types, by evaluating the proportion of counter‐ions for ligand carboxylate oxygens.
Prospective Assessment of Otologic Adverse Events due to Teprotumumab: Preliminary Results
Authors: Kay‐Rivest Emily , Belinsky Irina , Kozlova Anna , Byrd Erin , McMenomey Sean O. , Jethanamest Daniel 
Published: January 2023

AbstractObjectiveTo assess a series of patients receiving teprotumumab therapy and objectively quantify the rates of otologic adverse events.Study DesignA prospective cohort study of adult patients receiving teprotumumab between May 2020 and January 2022.SettingTertiary referral center.MethodsPrior to treatment initiation, an ototoxicity‐specific audiometric battery was completed, which included conventional audiometry (frequencies 250‐8000 Hz), ultrahigh‐frequency audiometry (9000‐20,000 Hz), tympanometry, speech discrimination scores, and distortion product otoacoustic emissions (DPOAEs). Testing was then repeated after treatment completion.ResultsIn total, 35 patients were recruited, with a median (range) age of 48.5 years (21‐74), and 8 (22.8%) were male. The most common subjective symptom reported was a hearing decline (25.7%), followed by aural fullness (17.1%) and tinnitus (14.3%). Fourteen patients had both pre‐ and posttreatment audiometric data. Among them, 3 patients (21.4%) were found to have changes in standard frequency audiometry, and 10 (71.4%) had changes in high‐frequency audiometry, with 2 patients having changes in both. Less than half (n = 5) of the 11 patients with changes in standard or high‐frequency pure tone hearing noted subjective hearing decline. Changes in DPOAE were noted in 4 patients out of 13 (30.7%). Two patients discontinued treatment due to hearing decline. Finally, 3 patients (8.6%) were diagnosed with patulous eustachian tube (PET) by an otolaryngologist, and another 3 patients are suspected to have PET based on symptom description during ophthalmologic follow‐up.ConclusionIn our cohort, a high incidence of otologic symptoms was found to be associated with teprotumumab usage. Subjective hearing decline, changes in ultrahigh‐frequency hearing as well as eustachian tube dysfunction may be encountered and suggest the potential ototoxicity of teprotumumab.
Hearing Outcomes in a Deintensification Trial of Adjuvant Therapy for HPV‐Related Oropharyngeal Squamous Cell Carcinoma
Authors: Lee David S. , Mahal Rajwant S. , Tharakan Theresa , Cathryn Collopy  , Kallogjeri Dorina , Thorstad Wade L. , Adkins Douglas R. , Oppelt Peter , Ley Jessica , Wick Cameron C. , Zevallos Jose 
Published: January 2023

AbstractObjectiveTo explore whether deintensification of adjuvant therapy reduces ototoxicity among patients with human papillomavirus (HPV)‐related oropharyngeal squamous cell carcinoma (OPSCC).Study DesignRetrospective cohort study.SettingSingle academic center.MethodsThe ototoxicity rate among adult patients with HPV‐related OPSCC enrolled in the Minimalist Trial (MINT), a prospective phase 2 trial of surgery followed by risk‐adjusted deintensified adjuvant therapy (42 Gy radiation given alone or with a single 100 mg/m2 dose of cisplatin), was compared to that among a historical cohort treated with standard adjuvant therapy (60‐66 Gy radiation with up to three 100 mg/m2 doses of cisplatin). Ototoxicity was defined as Common Terminology Criteria for Adverse Events v5.0 ≥ Grade 2. Mixed model analysis was performed to investigate the association between deintensified adjuvant therapy and treatment‐related hearing loss.ResultsA total of 29 patients (58 ears) were analyzed in the MINT cohort, and 27 patients (54 ears) in the historical cohort. The ototoxicity rate was 5% (n = 3/58 ears) in the MINT cohort and 46% (n = 25/54 ears) in the historical cohort (difference, 41%; 95% confidence interval [CI] = 27%‐56%). Patients in the MINT cohort demonstrated a 95% decrease in risk of ototoxicity compared to those in the historical cohort (adjusted odds ratio: 0.05, 95% CI = 0.01‐0.31). Differences in estimated marginal mean threshold shifts were statistically and clinically significant at frequencies ≥ 3 kHz.ConclusionThe deintensified adjuvant therapy given in MINT led to less ototoxicity than standard adjuvant therapy among patients with HPV‐related OPSCC.
The relationship between teacher stress and job satisfaction as moderated by coping
Authors: Woods Seth , Sebastian James , Herman Keith C. , Huang Francis L. , Reinke Wendy M. , Thompson Aaron M. 
Published: January 2023

AbstractThe present study investigated the relationship between teacher stress and job satisfaction, and examined the role of coping as a moderator. Based on the transactional and Coping‐Competence‐Context models of stress we expected that coping would serve as a protective factor in the relationship between teacher stress and their job satisfaction. Stress, coping, and satisfaction were measured using single‐item scales which are cost‐effective and practical instruments for measuring and monitoring teacher stress. A total of 2347 teachers from 93 schools formed the sample for this study. Regression analysis with job satisfaction as the outcome, and stress, coping, and their interaction entered separately were used to test the primary hypothesis. Results showed that there was a negative correlation between stress and job satisfaction, and coping had a significant moderating effect. Increasing levels of stress had less of a negative impact on job satisfaction for teachers with high coping ratings compared to those with average or low coping ratings. Overall, our results suggest that the development of coping skills could be beneficial for mitigating the effects of stress on job satisfaction.
Influence of traumatic lower‐limb amputation on physical activity, body composition, and cardiometabolic risks: A descriptive preliminary study
Authors: Ladlow Peter , Nightingale Thomas E. , McGuigan M. Polly , Bennett Alexander N. , Koumanov Francoise , Phillip Rhodri , Bilzon James L. J. 
Published: January 2023

AbstractBackgroundFollowing traumatic lower‐limb amputation (LLA), humans are predisposed to numerous unfavorable changes in health, including the development of secondary chronic health conditions such as metabolic disorders and cardiovascular disease.ObjectiveTo determine within and between group differences in cardiometabolic component risks, body composition, and physical activity (PA) in individuals with traumatic unilateral and bilateral LLA, compared to noninjured controls.DesignProspective observational cohort study.SettingA military complex trauma rehabilitation center.ParticipantsSixteen males with traumatic LLA (8 unilateral, mean age 30 ± 5 years and 8 bilateral, mean age 29 ± 3 years). Thirteen active age‐matched males with no LLA (28 ± 5 years) acted as controls and performed habitual activities of daily living.InterventionParticipants with LLA attended two 4‐week periods of inpatient rehabilitation, separated by two 6‐week periods of home‐based recovery.Main Outcome MeasuresVenous blood samples were taken prior to and following a 75 g oral glucose load, for determination of biomarkers, including insulin and glucose, at baseline and 20 weeks. Body composition (dual X‐ray absorptiometry) was measured at baseline, 10 weeks, and 20 weeks. Daily PA was recorded using a triaxial accelerometer for 7 days during inpatient rehabilitation and while at home. Energy expenditure was estimated using population‐specific equations.ResultsIndividuals with bilateral LLA demonstrated more unfavorable mean body composition values, lower PA, and increased cardiometabolic health risk compared to controls. Cardiometabolic syndrome was identified in 63% of individuals with bilateral LLA. No statistically significant differences in cardiometabolic component risk factors, body composition, and estimated daily PA were reported between unilateral LLA and control groups (p > .05). While at home, mean PA counts.day−1 reduced by 17% (p = .018) and 42% (p = .001) in the unilateral and bilateral LLA groups, respectively.ConclusionsDespite extensive inpatient rehabilitation, cardiometabolic component risks are elevated in individuals with bilateral LLA but are comparable between unilateral LLA and active noninjured control groups. Innovative strategies that improve/support the long‐term PA and cardiometabolic health of severely injured individuals with bilateral LLA are warranted.
Evaluation of a computer game‐assisted rehabilitation program for manual dexterity of children with cerebral palsy: Feasibility randomized control trial
Authors: Kanitkar Anuprita , Parmar Sanjay Tejraj , Szturm Tony Joseph , Restall Gayle , Rempel Gina Ruth , Sepehri Nariman , Naik Nilahri 
Published: January 2023

AbstractBackgroundThere is a need for innovation to improve the engagement and compliance of rehabilitation programs for children with upper extremity (UE) motor impairments due to cerebral palsy (CP); a computer games‐based rehabilitation platform (GRP) was developed to address this need. The GRP provides engaging task‐specific exercises targeting manual dexterity (object handling and manipulation).ObjectiveTo evaluate the therapeutic value and treatment effect size of an exercise program using the GRP in children with CP.MethodsA total of 63 children with CP, aged 4 to 10 years, were recruited. The Peabody Developmental Motor Scale‐2 (PDMS‐2) Grasp and Visual‐Motor Integration (VMI) subscores and Computer game‐based Upper Extremity (CUE) assessment of manual dexterity were used to assess participants before and after a 16‐week intervention program, delivered three times per week. The experimental group (XG) received a computer games‐based exercise program targeting object manipulation tasks. The active control arm (CG) consisted of task‐specific training similar to the tasks used in constrained induced movement therapy.ResultsThere were only a few dropouts during the 16‐week program, and compliance was high. Both groups showed significant improvements with medium to large effect sizes. Improvements in the PDMS‐2 Grasp and VMI subscores observed in the XG were significantly greater than that in the CG. There were significant improvements (p < .01) in PDMS‐2 grasp and VMI subscores for XG with moderate to large effect sizes (0.5–0.8). For CG, the Grasp and VMI subscores did improve but these changes were not statistically significant. There was a significant improvement observed in the majority of CUE object manipulation test scores for XG (p < .01) with moderate to large effect sizes (0.50–1.2) Although CG did show improvements in all CUE object manipulation test scores, the changes did not reach statistical significance (p < .01).ConclusionThis study demonstrates the utility of the GRP to practice a broad range of object manipulation tasks in children with CP. The present findings are positive and support further research and development. The long‐term effects of the GRP program in children with CP will need to be confirmed in a future randomized controlled trial. In addition to measures of structure and function, future trials should also include outcome measures such as health‐related quality of life and level of participation to validate the findings.
Assembly–disassembly switching of chiral sheet assembly for controlled circularly polarized luminescence
Authors: Dong Longlong , Lee Dawoon , Kim Yongju , Lee Myongsoo 
Published: January 2023

AbstractAlthough substantial advances have been made regarding synthetic chiral assemblies, obtaining the two‐dimensional (2D) system with a sophisticated supramolecular chirality remains challenging. Herein, we report the switchable supramolecular chiral 2D materials via donor‐acceptor interaction between a pyrene amphiphile and 7,7,8,8‐tetracyanoquinodimethane (TCNQ). The resulting supramolecular chiral sheets spontaneously disassemble when TCNQ is irreversibly photo‐reduced to its anion. Subsequent additions of TCNQ drive the disassembled molecules to repeatedly form optically active sheet structures, demonstrating that the assembled materials exist transiently only while TCNQ is supplied. The chiral sheets emit intense circularly polarized light with a large luminescence dissymmetric factor (glum).