bluuebunny/crossref_metadata_2025_split huggingface dataset:
Dynamic analysis and optimal control of cancer oncolytic virotherapy model with chemotherapy and immune response
Authors: Viriyapong Ratchada , Meehinkong Panisara 
Published: January 2023

Abstract
Oncolytic virotherapy is another therapeutic treatment for cancer and has been greatly interested recently by many researchers. In this study, a mathematical model of treating cancer by a combination of oncolytic virotherapy and chemotherapy is proposed. This model involves both immune response to cancer and virus. We derive three equilibrium points which are cancer-free, virus-free and cancer endemic. The stability of each equilibrium point is performed and we show that these equilibrium points are conditionally stable. Further, parameter analysis is established to explore which parameters playing role in cancer treatment overall. The results show that although chemotherapy is a successful treatment, with an addition of oncolytic virotherapy, it can help treatment more successful. Finally, optimal control problem is applied into the model by adding three controls which are immunotherapy control, virotherapy control and chemotherapy control to seek the best strategy in reducing cancer cells as much as possible. Numerical results of each strategy are carried out and they demonstrate that a combination of all three controls gives the best result in reducing cancer cells eventually.
2000 Mathematics Subject Classification: 34D20; 34D23; 34H05; 37N25
Studies on pre-fertilization crossability barriers between cultivated and wild species of pigeonpea [Cajanus cajan (L.) Millspaugh]
Authors: Verma Sanjay Kumar , Karn Anandi , Kumar Ravindra , Pant Usha , Arora Anju , Sharma Ram Kumar , Gaur Amit Kumar , Singh Indra Prakash 
Published: January 2023

Abstract
The present day commercially grown cultivars of pigeonpea are susceptible to pod borer, pod fly insect pest and a number of diseases those causes huge economic loss every year to the farmers. On the other hand wild relative of cultivated pigeonpea possess genes for insect pest/ diseases resistance besides having some other useful genes. Transfer of desirable gene from wild to cultivated pigeonpea is hindered by several pre fertilization barriers. The identification of pre fertilization barriers will help in the introgression of desirable genes for insect pest and diseases resistance into cultivated types for enhancing and stabilizing pigeonpea production. In present investigation crossability barriers between the cultivated Cajanus cajan (PADT-16 and Pusa-992) and wild species Cajanus scarabaeoides (ICP-15683, ICP-15703) and Cajanus platycarpus (ICP-15663) were examined by pre-fertilization parameters like pollen germination, pollen tube growth, and pollen tube abnormalities. In hand-selfing and intervarietal crosses mean pollen germination and pollen tube growth was higher than interspecific crosses. Strong pre-fertilization barriers were observed in C. cajan x C. platycarpus crosses. However in interspecific crosses involving C. platycarpus as female, low level of reproductive barriers were observed when crossed with C. scarabeoides compared to cross with C. cajan, though pollen tube reached ovule in both crosses. More pollen tube abnormalities were observed in interspecific crosses involving cultivated and species from tertiary gene pool. Molecular diversity among experimental material through SSR markers exhibited maximum diversity between cultivated C. cajan and wild C. platycarpus whereas closest relationship was observed between C.scarabeoides ICP-15683 and ICP-15703.
Tear fluid and Complement activation products in tears after ocular surgery.
Authors: Maehara Hiroki , Norikawa Koki , Tanaka Keiichiro , Kato Yutaka , Kasai Akihito , Omori Tomoko , Machida Takeshi , Sekine Hideharu , Sekiryu Tetsuju 
Published: January 2023

Abstract
Purpose
To investigate changes in the tear film complement activation products (CAPs) and ocular surface after vitrectomy combined with cataract surgery.
Methods
Forty-three patients (23 women; median age, 69 years) were enrolled in this prospective study and underwent phacoemulsification and vitrectomy. We measured Schirmer's test (ST) and CAPs in the tears at baseline, 4 days and 1 month after the surgery. Tears were collected in microtubes. The CAPs in the tear fluid were analyzed by cytometric bead array.
Results
A negative correlation was found between the ST and C3a (r2 = -0.37, P = 0.0015) at baseline. The median ST (8.5 mm) at baseline increased to 16 mm at 4days ( P < 0.001) and 10 mm at 1 month (P = 0.44). The C3a levels (1202 pg/ml) at baseline increased to 2753 pg/ml at 4 days (P < 0.001), and 1763 pg/ml at 1month (P = 0.049). The C4a levels (476 pg/ml) at baseline increased to 880 pg/ml at 4days (P < 0.001), and 657 pg/ml at 1 month (P = 0.013). The C5a levels (22.6 pg/ml) at baseline increased to 470.9 pg/ml at 4 days (P < 0.001), and 38.3 pg/ml (P = 0.0048). The surgical eyes were divided into the short ST group ( ≦ 10 mm, n = 22) and long ST group (> 10 mm, n = 21) based on the preoperative ST values. At 1 month postoperatively, the C3a levels was 2194 pg/ml in the preoperative short ST group and 1391 pg/ml in the long ST group, with significantly higher C3a concentrations in the short ST group (P < 0.001).
Conclusions
The CAPs levels in tears increased after vitrectomy combined with cataract surgery. A preoperative deficit in tear secretion might induce prolonged complement activation and delayed recovery of ocular surface parameters postoperatively.
The SmERF1b-like was involved in tanshinone biosynthesis of Salvia miltiorrhiza hairy root
Authors: Li Dan , Liu Yu , Bai Zhenqing , Yan Yan 
Published: February 2023

Abstract
Ethylene response factor (ERF) play an important role in the regulation of secondary metabolism in Salvia miltiorrhiza. Here, an AP2/ERF gene (named SmERF1b-like) was cloned from the cDNA library of S. miltiorrhiza. The SmERF1b-like had the ERF conserved domain，and it was highly expressed in leaves. It was responded to exogenous ethylene treatment. SmERF1b-like enriched in the nucleus. SmERF1b-like was bond to the GCC-box in the promoter of key tanshinones biosynthesis gene in S. miltiorrhiza. In the SmERF1b-like overexpressed hairy roots, the expression level of SmERF1b-like, SmCPS1 and SmKSL1 were significantly up-regulated, and the contents of tanshinone I and tanshinone IIA were significantly increased. While the SmERF1b-likegene silenced, the gene expression level and the content of tanshinones were not significantly different with overexpressed SmERF1b-like. It is showed that SmERF1b-like had the genetic function redundancy in biosynthesis regulation of tanshinone. Above all, SmERF1b-like recognized the GCC-box of key gene promoter in nucleus to promote the biosynthesis of tanshinone. This study provides the theoretical support for the use of plant genetic engineering strategies to improve S. miltiorrhiza resources.
A Novel Tetra-Primer ARMS-PCR for genotyping of the OPRM 1 gene rs1779791 variant associated with opioid use disorders
Authors: Wijekumar P. J. , Ranadeva N. D.K. , Jayamaha A. R. , Herath H.M.N.D.M , Noorden N. , Fernando S.S.N. 
Published: January 2023

Abstract
Objectives: SNV is a single nucleotide change that can occur at any point in the genome. It is regarded as a frequent genetic variant that can result in genetic diseases. Discovering genetic variants in individuals is one of the most essential jobs of genetic research. Researchers genotype SNVs using TaqMan technology, DNA microarray, MALDI-TOF mass spectrometry, and automated sequencing which is expensive and time-consuming. The OPRM1 gene rs1799971 (A118G) has been identified for its association with Opioid use disorder (OUD). The present study focused in developing a single step identification test using Tetra-Primer Amplification Refractory Mutation System-PCR (T-ARMS-PCR) in order to detect the presence of SNV OPRM1 rs1799971 (A118G). The present research was performed to optimize the protocol for the designed four primers and validate using a total of 52 buccal samples from volunteers who are currently under rehabilitation for the drug abuse disorder.
Results: The novel assay was successfully designed, tested, and validated using 52 DNA samples. The products of the T-ARMS PCR for rs1799971 contained 395bp as the control band, 186bp as G allele (variant) and 257bp as A allele (wild type) were observed in the gel image. The genotype frequencies for the OPRM1 gene rs1799971 (A118G) were 44% (22/52) of homozygous variant type (GG), 28.9% (15/52) of homozygous wild type (AA) and 28.9% (15/22) of heterozygous (AG). The minor allele (G) frequency was 56.7% and major allele (A) frequency was 43.3%.
Effect of using an electronic health record on interdisciplinary collaboration between professionals in care for youth, a mixed methods intervention study
Authors: Benjamins Janine , Vet Emely , Haveman-Nies Annemien 
Published: January 2023

Abstract
Background
An interdisciplinary client-accessible electronic child health record (EPR-Youth) has been developed in the Netherlands with the aim of enhancing interdisciplinary collaboration between preventive child healthcare and youth care. Interdisciplinary collaboration contributing to integrated family care plans should counteract the rising costs in Dutch care for youth. So far, limited research is available about the contribution of client-accessible health records to interdisciplinary collaboration in child and adolescent care worldwide.
Objective
To investigate whether the use of EPR-Youth contributes to interdisciplinary collaboration between professionals in youth care and child healthcare.
Methods
In a mixed methods design, two partly overlapping samples of professionals completed questionnaires before introduction of EPR-Youth (n = 117) and 24 months thereafter (n = 127). Five components of interdisciplinary collaboration (interdependency, creation of new professional activities, flexibility, collective ownership of goals, and reflection on processes) were assessed. Halfway through the study period, two focus group interviews were held with professionals (n = 12), investigating the contribution of EPR-Youth to interdisciplinary collaboration. A linear mixed model was used to analyse differences between pre- and post-test measurements. Qualitative data were analysed using thematic analysis.
Results
Professionals reported significantly more positive about flexibility after introduction of EPR-Youth (n = 106, EMM = 4.00, 95%CI = 3.86–4.14) than before (n = 97, EMM = 3.79, 95%CI = 3.65–3.92), F (1, 100.7) = 1.97, p = 0.05. For the other components of collaboration as well as overall, professionals scored slightly, although not significantly, more positive after introduction of EPR-Youth than before. In line with these outcomes, focus group participants reported that use of EPR-Youth enhanced the sense of ‘interdependency’ and ‘collective ownership of goals’ and contributed to the ‘creation of new professional activities’. At baseline, differences in level of interdisciplinary collaboration were found between organizations, F(2, 134.0) = 7.17, p = 0.001, and between municipalities, F(4,130.8) = 3.80, p = 0.006. These findings were confirmed by focus group participants. Professionals attributed organizational differences to different facilitation of interdisciplinary collaboration.
Conclusions
This study suggests that using EPR-Youth might foster interdisciplinary collaboration. The different levels of interdisciplinary collaboration between organizations underline that implementing an EPR alone does not contribute to interdisciplinary collaboration: a shared vision and organizational facilities are needed to further strengthen interdisciplinary collaboration.
Gene expression of Near-Isogenic Lines (NILs) Carrying Blast Resistance Genes Pi9 and Pi54 in the Background of Rice Cultivar Mushk Budji
Authors: Shafi Afshana , Khan Raheel Shafeeq , Mir Saba , Khan Gazala H. , Masoodi Khalid Z. , Sofi Najeebul Rehman , Mohidin Fayaz A. , Lone Javeed A. , Shikari Asif 
Published: January 2023

Abstract
Background
Kashmir valley, India is a homeland to rice landraces like Zag, Nunbeoul, Qadirbeigh, Kawkadur, Kamad, Mushk Budji, etc, generally characterized by short grains, aroma, earliness and cold tolerance. Mushk Budji is a commercially important speciality rice known for its taste and aroma, nonetheless, is extremely vulnerable to blast disease. Through the use of the marker-assisted backcrossing (MABC) approach, a set of 24 Near-isogenic lines (NILs) was created, and the lines with the highest background genome recovery were chosen. The expression analysis was carried out for the component genes and other eight pathway genes related to blast resistance.
Results
The major blast resistance genes Pi9 (from IRBL-9W) and Pi54(from DHMAS 70Q 164-1b) were incorporated following simultaneous-but-step-wise MABC. The NILs harbouring genes Pi9+Pi54, Pi9 and Pi54 expressed resistance to isolate (Mo-nwi-kash-32) under controlled and natural field conditions. The loci controlling ETI (effector triggered immunity) included the gene Pi9 and showed 61.18 and 60.27 fold change in relative gene expression in Pi54+Pi9and Pi9 carrying NILs against RP Mushk Budji. Pi54 was up regulated and showed 41 and 21 fold change in relative gene expression for NIL-Pi54+Pi9and NIL-Pi54, respectively. Among the pathway genes, LOC_Os01g60600 (WRKY 108) recorded 8 and 7.5 fold up regulation in Pi9and Pi54 NILs.
Conclusion
The NILs showed recurrent parent genome recovery (RPG) per cent of 81.67 to 92.54 and were on par in performance to recurrent parent Mushk Budji. The lines were utilized to study the expression of the loci controlling WRKYs, peroxidases and chitinases that confer overall ETI response.
Prediction of Risk and Clinical Outcome of cuproptosis in Lung Squamous carcinoma
Authors: Zhang Yangyang , Zhou Jia , Li Hong , Liu Yaobang , Li Jinping 
Published: January 2023

Abstract
Background: Lung squamous cell carcinoma (LUSC) is a common histopathologic type of lung cancer, and chemotherapy is still the main means for advanced LUSC. Cuproptosis is a newly discovered form of cell death different from known programmed apoptosis, which regulates the proliferation and progression of tumor cells. However, the molecular mechanism and prognosis of cuproptosis-related genes (CRGs) in LUSC have not yet been reported.
Methods: RNA sequencing profiles and related clinical data of LUSC were downloaded from The Cancer Genome Atlas (TCGA) and Gene Expression Omnibus (GEO) databases and combined into a new LUSC cohort. The data were processed using multiple R packets, and CRGs associated with the prognosis of pulmonary squamous carcinoma were screened based on differences in gene expression. Through the interaction network analysis of tumor mutation load, copy number variation and CRGs, the patients were divided into two subtypes according to the expression of CRGs, and three different gene clusters according to the difference in gene expression. The key differential genes were screened to construct prognostic markers, and the correlation between LUSC immune cell infiltration and immunity was further analyzed. A more accurate nomogram map was constructed by risk score and clinical factors. In addition, drug sensitivity analysis was performed on CRGs of LUSC.
Results: Patients were divided into two cuproptosis-related subtypes and subsequently regrouped into three gene clusters, showing different immune infiltrations. The results of risk score showed that compared with the low-score risk group, the high-score risk group had higher tumor microenvironment score, lower frequency of tumor mutational burden, poor prognosis. At the same time, high-score risk group had higher sensitivity to chemotherapy of cisplatin, doxorubicin, etoposide, paclitaxel, vinorelbine and other drugs.
Conclusions: We developed a new scoring system to predict the prognosis and immune status of patients with LUSC. This feature shows a satisfactory predictive effect and has the potential to guide patients' integrative treatment.
Flexible batch electrodialysis for low-cost solar-powered brackish water desalination
Authors: He Wei , Henaff Anne-Claire Le , Amrose Susan , Buonassisi Tonio , Peters Ian , Winter Amos 
Published: January 2023

Abstract
There are over 1.6 billion people living in rural, water-stressed areas across the world. Brackish groundwater desalination can expand these stressed freshwater supplies. Compared to conventional reverse osmosis (RO), electrodialysis reversal (EDR) can reduce water wastage and energy consumption in brackish water desalination. The improved energy efficiency and water recovery makes solar photovoltaic (PV)-powered EDR systems promising solutions for locations where grid access is restricted or unavailable. However, state-of-the-art PV-EDR is still prohibitively expensive for cost-constrained communities, largely due to the use of batteries for addressing solar power's intermittence. To reduce a system's dependence on batteries, here we present a novel time-variant EDR technology that can flexibly vary pumping and EDR power to match available intermittent solar power while maximizing the desalination rate. We experimentally demonstrated that the flexible system was able to directly utilize 77% of the available solar energy on average, a 91% improvement compared to the reference conventional PV-EDR operation which operated at constant flowrate and EDR voltage. The flexible system also requires 92% less battery capacity. Using a village-scale desalination case study in India, shifting from constant to flexible operation of PV-EDR systems can reduce the levelized cost of water by 22% and make the technology cost-competitive with currently used on-grid, village-scale RO desalination solutions. Cost projections for EDR membranes and brine disposal show that in the future, PV-EDR could produce water at 60\% the cost achieved by on-grid RO, which would provide an improved sustainable solution for addressing water scarcity in remote areas.
Overlaps between the MREL and macroprudential capital buffers: a dynamic insight through stress tests
Authors: Pfeifer Lukáš 
Published: February 2023

AbstractThe article studies the impacts of the minimum requirement for own funds and eligible liabilities (MREL) on the usability of macroprudential capital buffers and any capital surplus for absorbing losses and lending to the economy through the solvency stress test of the Czech banking sector. The introduction of the MREL makes it more difficult to assess the resilience of the banking sector, especially during long adverse economic episodes in banks that use internal models to manage credit risk. The results indicate that under these circumstances, there is, i) a decrease in the usability of capital buffers, ii) a relatively early trigger of the new maximum distributable amount related to the MREL (M-MDA) regime, potentially associated with a new form of stigma effect, iii) and there may be MREL non-compliance. All these findings should be (i) incorporated into the solvency stress testing framework and published regularly, (ii) taken into account by the competent authorities (microprudential, macroprudential, resolution) to limit capital buffer overlaps and increase the distance to breaching the M-MDA.
Reconstruction of cardiac images using the confocal microscopy
Authors: Hamed A. M. , Al-Saeed T. 
Published: January 2023

Abstract
An aperture in the form of a cardioid shape is suggested in the processing of cardiac images using the confocal microscope. The impulse response or the Point Spread Function (PSF) corresponding to the cardiac images and the cardioid aperture have been computed using the FFT algorithm. The effect of the image rotation on the PSF was investigated. In addition, the autocorrelation corresponding to the cardiac images was computed and compared with the apertures of circular and cardioid shapes. In addition, the cross-correlation of aligned and rotated images was investigated. Finally, reconstruction of the cardiac images using the confocal scanning microscope was obtained considering the circular and cardioid shapes of apertures. The MATLAB code was used in the formation of all images and plots.
Evolutionary Algorithm for Optimized CNN Architecture Search Applied to Real-Time Boat Detection in Aerial Images
Authors: Zerrouk Ilham , Moumen Younes , Khiati Wassim , El-Habchi Ali , Berrich Jamal , Bouchentouf Toumi 
Published: January 2023

Abstract
When processing the detection of boats on aerial images by neural networks, we have always been concernedabout the execution time of these networks in the equipment on board the Unmanned AerialVehicle (UAV). The UAV, throughout its mission, will capture images that must be processed in realtime. For this, an execution time optimized network is essential. This article proposes a method forsearching for optimized detection networks, for a given dataset, using an evolutionary algorithm. Theresearch uses mutations as a mechanism of evolution that affect the structure of the network and thehyper-parameters of its layers. Its fitness function allows the choice of architectures that are not verygreedy in terms of operations favoring small networks whose advantages are to be fast and quick totrain and thus to accelerate the search algorithm. Thanks to this method we were able to obtain detectionnetworks with performance similar to the initial network (parent) but with much fewer operationsallowing considerable gains in terms of execution times in an environment embedded on a drone.
Halodecarboxylative-dimethoxylation of 2,3-diarylacrylic acid using (diacetoxyiodo)benzene and Et4NBr: Synthesis of 2-bromo-1,2- dimethoxy-1,2-diarylethane
Authors: Opai Imran A. , Shirsath Prakash D. , Dudhe Payal S. , Karade Nandkishor N. 
Published: February 2023

Abstract
Synthesis of 2,3-diaryl acrylic acid is accomplished using the Perkin reaction between aromatic aldehyde and variously substituted phenyl acetic acids in presence of acetic anhydride and triethylamine at reflux condition. The reaction of resulting 2,3-diaryl acrylic acids with (diacetoxyiodo)benzene and tetraethylammonium bromide in MeOH/H2O forms 2-bromo-1,2-dimethoxy-1,2-diarylethane in moderate to good yields. The structure of the synthesized products was characterized by FTIR, 1H, 13C NMR spectroscopy and mass spectrometry. This is an unusual reaction observed for α,β-unsaturated carboxylic acids under transition metal-free oxidative condition.
A coupled system of q-fractional Lane-Emden problem involving three sequential q-fractional derivatives with stability inequality
Authors: Houas Mohamed , Samei Mohammad Esmael , Rezapour Shahram 
Published: February 2023

Abstract
In the current manuscript, we consider a sequential q-fractional Lane-Emden system involving Riemann-Liouville and Caputo type fractional q-derivatives. We prove the uniqueness of solutions by means Banach fixed point theorems. Then, to demonstrate theexistence of at least one solution, we use Schaefer's fixed point theorem. Also we define and prove two types of Ulam-Hyers for the proposed system. Finally, an illustrative example is proposed.
(2010) Mathematics Subject Classifications: 39A13, 39B72, 30C45.