bluuebunny/crossref_metadata_2025_split huggingface dataset:
Spatial Transcriptomics Depict Ligand–Receptor Cross-talk Heterogeneity at the Tumor-Stroma Interface in Long-Term Ovarian Cancer Survivors
Authors: Ferri-Borgogno Sammy , Zhu Ying , Sheng Jianting , Burks Jared K. , Gomez Javier A. , Wong Kwong Kwok , Wong Stephen T.C. , Mok Samuel C. 
Published: February 2023

Abstract

Advanced high-grade serous ovarian cancer (HGSC) is an aggressive disease that accounts for 70% of all ovarian cancer deaths. Nevertheless, 15% of patients diagnosed with advanced HGSC survive more than 10 years. The elucidation of predictive markers of these long-term survivors (LTS) could help identify therapeutic targets for the disease, and thus improve patient survival rates. To investigate the stromal heterogeneity of the tumor microenvironment (TME) in ovarian cancer, we used spatial transcriptomics to generate spatially resolved transcript profiles in treatment-naïve advanced HGSC from LTS and short-term survivors (STS) and determined the association between cancer-associated fibroblasts (CAF) heterogeneity and survival in patients with advanced HGSC. Spatial transcriptomics and single-cell RNA-sequencing data were integrated to distinguish tumor and stroma regions, and a computational method was developed to investigate spatially resolved ligand–receptor interactions between various tumor and CAF subtypes in the TME. A specific subtype of CAFs and its spatial location relative to a particular ovarian cancer cell subtype in the TME correlated with long-term survival in patients with advanced HGSC. Also, increased APOE-LRP5 cross-talk occurred at the stroma-tumor interface in tumor tissues from STS compared with LTS. These findings were validated using multiplex IHC. Overall, this spatial transcriptomics analysis revealed spatially resolved CAF-tumor cross-talk signaling networks in the ovarian TME that are associated with long-term survival of patients with HGSC. Further studies to confirm whether such cross-talk plays a role in modulating the malignant phenotype of HGSC and could serve as a predictive biomarker of patient survival are warranted.

Significance:
Generation of spatially resolved gene expression patterns in tumors from patients with ovarian cancer surviving more than 10 years allows the identification of novel predictive biomarkers and therapeutic targets for better patient management.
See related commentary by Kelliher and Lengyel, p. 1383
Deacetylation of Septin4 by SIRT2 (Silent Mating Type Information Regulation 2 Homolog-2) Mitigates Damaging of Hypertensive Nephropathy
Authors: Zhang Ying , Zhang Naijin , Zou Yuanming , Song Chunyu , Cao Kexin , Wu Boquan , You Shilong , Lu Saien , Wang Dong , Xu Jiaqi , Huang Xinyue , Zhang Pengyu , Fan Zihao , Liu Jingwei , Cheng Zhongyi , Zhang Zhe , Kong Chuize , Cao Liu , Sun Yingxian 
Published: February 2023

Background:
Hypertension can lead to podocyte damage and subsequent apoptosis, eventually resulting in glomerulosclerosis. Although alleviating podocyte apoptosis has clinical significance for the treatment of hypertensive nephropathy, an effective therapeutic target has not yet been identified. The function of septin4, a proapoptotic protein and an important marker of organ damage, is regulated by post-translational modification. However, the exact role of septin4 in regulating podocyte apoptosis and its connection to hypertensive renal damage remains unclear.

Methods:

We investigated the function and mechanism of septin4 in hypertensive nephropathy to discover a theoretical basis for targeted treatment. Mouse models including Rosa 26 (Gt(ROSA)26Sor)-
SIRT2
(silent mating type information regulation 2 homolog-2)-Flag-TG (transgenic) (
SIRT2
-TG) mice
SIRT2
-knockout, and
septin4
-K174Q mutant mice, combined with proteomic and acetyl proteomics analysis, followed by multiple molecular biological methodologies, were used to demonstrate mechanisms of SIRT2-mediated deacetylation of septin4-K174 in hypertensive nephropathy.

Results:

Using transgenic
septin4
-K174Q mutant mice treated with the antioxidant Tempol, we found that hyperacetylation of the K174 site of septin4 exacerbates Ang II (angiotensin II)– induced hypertensive renal injury resulting from oxidative stress. Proteomics and Western blotting assays indicated that septin4-K174Q activates the cleaved-PARP1 (poly [ADP-ribose] polymerase family, member 1)-cleaved-caspase3 pathway. In septin4-knockdown human renal podocytes, septin4-K174R, which mimics deacetylation at K174, rescues podocyte apoptosis induced by Ang II. Immunoprecipitation and mass spectrometry analyses identified SIRT2 as a deacetylase that interacts with the septin4 GTPase domain and deacetylates septin4-K174. In
Sirt2
-deficient mice and SIRT2-knockdown renal podocytes, septin4-K174 remains hyperacetylated and exacerbates hypertensive renal injury. By contrast, in Rosa26-Sirt2-Flag (SIRT2-TG) mice and SIRT2-knockdown renal podocytes reexpressing wild-type SIRT2, septin4-K174 is hypoacetylated and mitigates hypertensive renal injury.

Conclusions:
Septin4, when activated through acetylation of K174 (K174Q), promotes hypertensive renal injury. Septin4-K174R, which mimics deacetylation by SIRT2, inhibits the cleaved-PARP1-cleaved-caspase3 pathway. Septin4-K174R acts as a renal protective factor, mitigating Ang II–induced hypertensive renal injury. These findings indicate that septin4-K174 is a potential therapeutic target for the treatment of hypertensive renal injury.
Emergence in Artificial Life
Authors: Gershenson Carlos 
Published: February 2023

AbstractEven when concepts similar to emergence have been used since antiquity, we lack an agreed definition. However, emergence has been identified as one of the main features of complex systems. Most would agree on the statement “life is complex.” Thus understanding emergence and complexity should benefit the study of living systems. It can be said that life emerges from the interactions of complex molecules. But how useful is this to understanding living systems? Artificial Life (ALife) has been developed in recent decades to study life using a synthetic approach: Build it to understand it. ALife systems are not so complex, be they soft (simulations), hard (robots), or wet(protocells). Thus, we can aim at first understanding emergence in ALife, to then use this knowledge in biology. I argue that to understand emergence and life, it becomes useful to use information as a framework. In a general sense, I define emergence as information that is not present at one scale but present at another. This perspective avoids problems of studying emergence from a materialist framework and can also be useful in the study of self-organization and complexity.
An Electronic Bandoneon with a Dynamic Sound Synthesis System Based
on Measured Acoustic Parameters
Authors: Ramos Juan , Calcagno Esteban , Vergara Ramiro , Rizza Joaquín , Riera Pablo 
Published: February 2023

Abstract
The bandoneon is a distinctive free-reed instrument with profound ties to tango culture and Latin American music. The scarcity of manufacturers and the related high retail prices, however, are restricting access to the instrument for new generations of musicians. By combining modern technologies and scientific research, the Bandoneon 2.0 project aims to create an expressive and accessible new version of the instrument.
In this article, we present an electronic bandoneon with a custom sound synthesis system. We also present an acoustic measurement system with which we analyzed the sound and air pressure signals of an acoustic bandoneon. Through this, we characterized several sound attributes that are utilized in a synthesis model made in Faust DSP. Combining the controller interface and the custom synthesizer, the electronic bandoneon we created can achieve a good level of expressiveness and engagement for the performer. We aim to produce an instrument that can be used in recreational, academic, and professional contexts to address the current sociocultural demand.
An Analytical Approach to the Scenic Realization of Electroacoustic Music: The Theatricality of Lumínico
Authors: Eisner Guillermo 
Published: February 2023

Abstract
The present work studies the scenic realizations of the ensemble called Lumínico (Rodrigo Sigal and Alejandro Escuer) from the perspective of intermediality, performance theory, musicality, and theatricality. Lumínico's proposal offers, as sound and audiovisual artists, an alternative to the experience of the traditional chamber music and electroacoustic concert, both to themselves and to the audience. In the field of music, it blurs the binary distinction between artistic work and audience by transferring the creation of the uninterrupted concert to the very moment of its execution and reception. This way, a Lumínico concert generates a unique, unrepeatable, and ephemeral event that emerges thanks to the characteristics of the scenic realization, starring the ensemble members and the audience.
Construction of nest mound and preference for it during relocation in an Indian ant Diacamma indicum
Authors: Bhattacharyya Kushankur , Annagiri Sumana 
Published: February 2023

Abstract
Ants living in subterranean nests face the challenge of nest flooding and require to combat this recurring issue. The tropical Ponerine ant species, Diacamma indicum, living in simple nests with a single chamber, was used in the current study to examine mound building in the lab. Upon stimulating rain ( colonies) they built significantly larger nest mounds as compared to controls. Nest mounds proved to be important to colonies that had experienced rain while relocating. Relocating colonies showed significantly higher preference for new nests with mounds (12/13) when choosing between two equidistant, similar quality potential new nests in contrast to control relocations. To the best of our knowledge this study for the first-time documents mound building behaviour in any Ponerine species in laboratory conditions and introduces nest mounds as another architectural feature of interest to relocating colonies.
An exploration of the concept of mental health recovery: Insights from practitioners and trainees
Authors: Badu Naomi , Usher Kim , Rice Kylie , Schutte Nicola , Islam Md Shahidul 
Published: February 2023

Background Recovery concepts were conceived by, and for, people living with mental health issues. Practitioners are key in translating recovery principles into action. Therefore, practitioners’ perceptions should be routinely assessed to facilitate in-service training, professional development, and curriculum redesign. Aim To explore practitioners’ and trainees’ current perspectives regarding the concept of recovery. Methods The study used a qualitative exploratory design with in-depth interviews to explore ten fully trained practitioners and eight trainees’ perspectives on recovery. Results The findings revealed a shared understanding of recovery among practitioners and trainees. Many responses explained recovery as a process involving internal and external conditions, however, some revealed medically oriented perspectives and/or were unclear about recovery terminologies. Conclusions There is convergence and growing acceptance of the recovery practice principles among practitioners and trainees. However, the results suggest that the recovery principles/terminologies have not yet been universally adopted. Implications for practice and research Continuous training on recovery principles is needed, incorporating emerging terminologies and principles. Also, education providers are encouraged to continue to review their training to incorporate emerging issues and trends in recovery-oriented practice. Finally, research is needed to enhance the development and delivery of recovery-focused practice.
Effects of insufficient sleep on sensorimotor processing in migraine: A randomised, blinded crossover study of event related beta oscillations
Authors: Mykland Martin Syvertsen , Uglem Martin , Bjørk Marte-Helene , Matre Dagfinn , Sand Trond , Omland Petter Moe 
Published: February 2023

Background Migraine has a largely unexplained connection with sleep and is possibly related to a dysfunction of thalamocortical systems and cortical inhibition. In this study we investigate the effect of insufficient sleep on cortical sensorimotor processing in migraine. Methods We recorded electroencephalography during a sensorimotor task from 46 interictal migraineurs and 28 controls after two nights of eight-hour habitual sleep and after two nights of four-hour restricted sleep. We compared changes in beta oscillations of the sensorimotor cortex after the two sleep conditions between migraineurs, controls and subgroups differentiating migraine subjects usually having attacks starting during sleep and not during sleep. We included preictal and postictal recordings in a secondary analysis of temporal changes in relation to attacks. Results Interictally, we discovered lower beta synchronisation after sleep restriction in sleep related migraine compared to non-sleep related migraine (p=0.006) and controls (p=0.01). No differences were seen between controls and the total migraine group in the interictal phase. After migraine attacks, we observed lower beta synchronisation (p<0.001) and higher beta desynchronisation (p=0.002) after sleep restriction closer to the end of the attack compared to later after the attack. Conclusion The subgroup with sleep related migraine had lower sensorimotor beta synchronisation after sleep restriction, possibly related to dysfunctional GABAergic inhibitory systems. Sufficient sleep during or immediately after migraine attacks may be of importance for maintaining normal cortical excitability.
Efficacy, safety and indirect comparisons of lasmiditan, rimegepant, and ubrogepant for the acute treatment of migraine: A systematic review and network meta-analysis of the literature
Authors: Puledda Francesca , Younis Samaira , Huessler Eva-Maria , Haghdoost Faraidoon , Lisicki Marco , Goadsby Peter J , Tassorelli Cristina 
Published: February 2023

Background We performed a random-effects network meta-analysis to study the efficacy and safety of newly developed drugs for the acute treatment of migraine attacks. Methods MEDLINE via PubMed, Embase and The Cochrane Register of Controlled Trials were searched from inception to 11 February 2022. Phase 3 randomized controlled trials examining all formulations of lasmiditan, rimegepant and ubrogepant for the acute treatment of adults with migraine, were included. Data were extracted following the PRISMA guidelines. Results Seven studies (SAMURAI, SPARTAN, CENTURION, Study 302, Study 303, ACHIEVE I and II) involving n = 12,859 patients were included. All treatments were superior in efficacy to placebo. Lasmiditan 200 mg showed the highest two-hour pain freedom, while two-hour freedom from most bothersome symptom was equally achieved by the higher doses of lasmiditan (100 and 200 mg), rimegepant and the higher doses of ubrogepant (50 and 100 mg). The odds of treatment-emergent adverse events were greatest with all doses of lasmiditan. Conclusion Lasmiditan 200 mg was the most effective intervention in the treatment of migraine attacks, although it was associated with high degrees of dizziness, nausea and somnolence. Rimegepant showed slightly lower, but similar efficacy rates to lasmiditan. Ubrogepant had overall the best tolerability profile. These conclusions are limited by the absence of head-to-head comparisons, limitations of individual trials and of the meta-analysis methodology itself. PROSPERO trial registration: CRD42022308224
Safety and tolerability of monoclonal antibodies targeting the CGRP pathway and gepants in migraine prevention: A systematic review and network meta-analysis
Authors: Messina Roberta , Huessler Eva-Maria , Puledda Francesca , Haghdoost Faraidoon , Lebedeva Elena R , Diener Hans-Christoph 
Published: February 2023

Background Direct comparisons of the tolerability and safety of migraine preventive treatments targeting the calcitonin gene-related peptide pathway are lacking. This study aimed to compare the safety and tolerability of anti-calcitonin gene-related peptide monoclonal antibodies and gepants in migraine prevention. Methods A network meta-analysis of phase 3 randomized controlled trials assessing the safety and tolerability of anti-calcitonin gene-related peptide monoclonal antibodies (erenumab, eptinezumab, fremanezumab, or galcanezumab) and gepants (atogepant, rimegepant) in migraine prevention was performed. Primary outcomes were treatment-emergent adverse events and serious adverse events. Secondary outcomes included any adverse events, adverse events leading to treatment discontinuation and individual adverse events. Results We included 19 randomized controlled trials, comprising 14,584 patients. Atogepant 120 mg (OR 2.22, 95% CI [1.26, 3.91]) and galcanezumab 240 mg (OR 1.63, 95% CI [1.33, 2.00]) showed the largest odds of treatment-emergent adverse events compared to placebo. While eptinezumab 30 mg had greater odds of adverse events leading to treatment discontinuation (OR 2.62, 95% CI [1.03,6.66]). No significant differences in serious adverse events were found between active treatments and placebo. Eptinezumab was associated with the lowest odds of treatment-emergent adverse events and serious adverse events compared to placebo, whereas erenumab was associated with the lowest odds of any adverse events and quarterly fremanezumab with the lowest odds of treatment discontinuation due to adverse events. Conclusion Monoclonal antibodies targeting the calcitonin gene-related peptide pathway and gepants are a safe and well tolerated option for migraine prevention.
Reduced cardiovascular risk score following bilateral cataract phacoemulsification surgery: A retrospective observational cohort study
Authors: Matias Maria João , Alves-Peixoto Pedro , Miranda Vítor , Aguiar Catarina Pestana , Ambrósio João , Rocha António , Chibante-Pedro João 
Published: February 2023

Objective To assess the cardiovascular risk (CV risk) change following bilateral phacoemulsification cataract surgery. Methods We performed a retrospective observation cohort study on 112 selected patients who underwent uncomplicated bilateral cataract surgery at Centro Hospitalar de Entre o Douro e Vouga (CHEDV) between 2018 and 2019. This patient cohort was further subdivided in 2 different groups: Good VA – no to mild visual impairment, ≤0.48 LogMAR; Bad VA – moderate to severe visual impairment, >0.48 LogMAR. We compared the changes in the CV risk score components in our patient cohort and between subgroups Good VA and Bad VA, before and after surgery, using paired t-test or Wilcoxon rank-sum test, and repeated measures ANOVA with Tukey post-hoc tests, respectively. Visual Acuity (VA) before and after surgery was correlated with the patients’ CV risk score. At last, linear regression models were built to explain changes in CV risk variables considering the change in VA. Results Cataract surgery resulted in improved VA. Notably, following surgery our patient cohort showed reduced low-density lipoprotein (LDL) levels after surgery, from 111.17±36.26 mg/dL to 104.22±37.53 mg/dL, and reduced systolic arterial pressure (SAP), from 139.1±15.0 mmHg to 133.7±12.0 mmHg. Ultimately, this translated to an improved CV risk score within 6 months of cataract surgery, from 17.39±11.44% to 16.51±11.27%. Of note, these improvements were mostly present in the Bad VA group of patients, where baseline VA and incidence of dyslipidemia were worse. Conclusion Our results suggest that phacoemulsification cataract surgery may be an important tool in addressing CV risk.
Subsidence Rates Associated With Porous 3D-Printed Versus Solid Titanium Cages in Transforaminal Lumbar Interbody Fusion
Authors: Toop Nathaniel , Dhaliwal Joravar , Grossbach Andrew , Gibbs David , Reddy Nihaal , Keister Alexander , Mallory Noah , Xu David , Viljoen Stephanus 
Published: February 2023

Study Design Retrospective Cohort Study. Objective To determine whether 3D-printed porous titanium (3DPT) interbody cages offer any clinical or radiographic advantage over standard solid titanium (ST) interbody cages in transforaminal lumbar interbody fusions (TLIF). Methods A consecutive series of adult patients undergoing one- or two-level TLIF with either 3DPT or ST “banana” cages were analyzed for patient reported outcome measures (PROMs), radiographic complications, and clinical complications. Exclusion criteria included clinical or radiographic follow-up less than 1 year. Results The final cohort included 90 ST interbody levels from 74 patients, and 73 3DPT interbody levels from 50 patients for a total of 124 patients. Baseline demographic variables and comorbidity rates were similar between groups ( P > .05). Subsidence of any grade occurred more frequently in the ST group compared with the 3DPT group (24.4% vs 5.5%, respectively, P = .001). Further, the ST group was more likely to have higher grades of subsidence than the 3DPT group ( P = .009). All PROMs improved similarly after surgery and revision rates did not differ between groups (both P > .05). On multivariate analysis, significant positive correlators with increasing subsidence grade included greater age ( P = .015), greater body mass index ( P = .043), osteoporosis/osteopenia ( P < .027), and ST cage type ( P = .019). Conclusions When considering interbody material for TLIF, both ST and 3DPT cages performed well; however, 3DPT cages were associated with lower rates of subsidence. The clinical relevance of these findings deserves further randomized, prospective investigation.
Inverted Modelling: An Effective Way to Support Motion Planning of Legged Mobile Robots
Authors: Zhao Chenyao , Guo Weizhong 
Published: February 2023

AbstractThis paper presents an effective way to support motion planning of legged mobile robots—Inverted Modelling, based on the equivalent metamorphic mechanism concept. The difference from the previous research is that we herein invert the equivalent parallel mechanism. Assuming the leg mechanisms are hybrid links, the body of robot being considered as fixed platform, and ground as moving platform. The motion performance is transformed and measured in the body frame. Terrain and joint limits are used as input parameters to the model, resulting in the representation which is independent of terrains and particular poses in Inverted Modelling. Hence, it can universally be applied to any kind of legged robots as global motion performance framework. Several performance measurements using Inverted Modelling are presented and used in motion performance evaluation. According to the requirements of actual work like motion continuity and stability, motion planning of legged robot can be achieved using different measurements on different terrains. Two cases studies present the simulations of quadruped and hexapod robots walking on rugged roads. The results verify the correctness and effectiveness of the proposed method.