bluuebunny/crossref_metadata_2025_split huggingface dataset:
Effects of synbiotic supplementation on the health-related quality of life in women with polycystic ovary syndrome. A randomized, triple-blind, placebo-controlled trial
Authors: Hariri Zahra , Yari Zahra , Hoseini Sedighe , Abhari Khadijeh , Sohrab Golbon 
Published: May 2023

Abstract
Background.
There are complicated mechanisms that link the disruption of the gut microbiome to the symptoms and complications of polycystic ovary syndrome (PCOS). In this study, an attempt was made to assess the effects of synbiotics on the health-related quality of life (HRQoL) in PCOS patients.
Methods.
Fifty-six women with PCOS were enrolled in a triple-blind controlled trial for 12 weeks. They were randomly assigned to receive a daily 2g synbiotic sachets (containing Bacillus coagulans (GBI-30), Lactobacillus rhamnus, Lactobacillus helveticus, and fructooligosaccharide) (n = 28) or placebo (n = 28). To evaluate the impact on the HRQoL, patients were required to fill 26-Item Polycystic Ovary Syndrome Health-Related Quality of Life Questionnaire (PCOSQ-26), 12-Item Short-Form Health Survey (SF-12) and Perceived Stress Scale (PSS-10) pre and post the intervention.
Results.
Finally, statistical analyses were performed on 52 patients who finished the trial. Synbiotic supplementation improved the scores of emotional (P = 0.044), body hair (P = 0.016), weight (P = 0.033) and infertility domains (P = 0.027) of PCOSQ-26 compared to placebo group. The physical score within SF-12 also had a significant enhancement (P = 0.035). No significant improvement was seen in the PSS-10 score at the end of the trial.
Conclusion.
This study illustrated the advantageous effects of synbiotics on the health-related quality of life in PCOS patients. Further studies are required to confirm our findings.
Trial registration:
http://www.irct.ir : IRCT20211108053007N1; date of registration: 2023-02-14
Late infection of cranioplasty presenting with extradural abscess thirty years after surgery: a case report
Authors: Perillo Teresa , Fedele Marina , D’Onofrio Giovanni , Serino Antonietta , Manto Andrea 
Published: May 2023

Abstract
Introduction: Delayed infection of cranioplasty is a rare complication which usually occurs 3 to 10 months after surgery. It causes subgaleal, extradural and/or subdural abscess, with accompanied local signs of infection. Typically, systemic manifestations are absent and laboratory tests are negative.
Case Presentation: We describe a case of late infection of the cranioplasty occurring 30 years after surgery, which has never been reported before in the scientific literature. Conclusions: Thus, in the appropriate clinical scenario, it is important to consider this entity among the differential diagnosis even decades after cranioplasty.
Enhancing feedback by health coaching: The Effectiveness of Mixed Methods Approach to Long-Term Physical Activity Changes in Nurses. An Intervention Study
Authors: Nerek Agnieszka , Wesołowska-Górniak Katarzyna , Czarkowska-Pączek Bożena 
Published: May 2023

Abstract
Background: Although knowledge of the barriers and motivators to physical activity participation among nurses is increasing, the factors influencing motivation methods’ effectiveness are not completely defined. This study aimed to identify the sociodemographic, occupational, and health-related factors that influence the effectiveness of motivation methods in increasing the level of daily physical activity among nurses.
Methods: This study was based on an intervention study protocol. All registered nurses in clinical settings were invited to participate in the study.
The study involved 71 professionally active nurses. A self-reported questionnaire was used to collect sociodemographic and employment data. The level of physical activity was assessed using the International Physical Activity Questionnaire, and the daily number of steps was assessed using a pedometer. Body composition was measured using a bioimpedance method, and the 5-year risk of cardiovascular events was assessed using the Harvard Score. The intervention included self-monitoring daily steps using a pedometer and completing a diary daily for one month. Additionally, a few-minute speech was sent to each participant via email on the intervention’s 7th, 14th, and 21st days.
Results: The analysis revealed a higher value of physical activity recorded in the follow-up compared to the initial and final measurement in the Recreation domain [Met] (p < 0.001) and a higher value of daily steps in the follow-up compared to the final measurement (p = 0.005). Participants with a higher Harvard Score were more likely to increase their daily number of steps (OR = 6.025; 95% CI = 1.70-21.41), and nurses working in hospital wards were less likely to do so (OR = 0.002; 95% CI = 0.00-0.41).
Conclusions: Recommendations for physical activity in the nursing population should focus on increasing leisure time physical activity and regular risk assessment of cardiovascular events. A mixed methods approach, such as feedback enhanced by health coaching, effectively achieves long-term physical activity changes in nurses.
Immunoassay-based quantification of full-length peptidylglycine alpha-amidating monooxygenase in human plasma
Authors: Ilina Yulia , Kaufmann Paul , Melander Olle , Press Michaela , Thuene Katrin , Bergmann Andreas 
Published: May 2023

Abstract
A one-step sandwich chemiluminescence immunometric assay (LIA) was developed for a quantification of bifunctional peptidylglycine-α-amidating monooxygenase (PAM) in human plasma (PAM-LIA). PAM is responsible for the activation of more than half of known peptide hormones through C-terminal α-amidation. The assay employed antibodies targeting specific catalytic PAM-subunits, peptidylglycine alpha-hydroxylating monooxygenase (PHM) and peptidyl-alpha-hydroxyglycine alpha-amidating lyase (PAL), to ensure detection of full-length PAM. The PAM-LIA assay was calibrated with a human recombinant PAM enzyme and achieved a detection limit of 189 pg/mL and a quantification limit of 250 pg/mL. The assay demonstrated good inter-assay (6.7%) and intra-assay (2.2%) variabilities. It exhibited linearity when accessed by gradual dilution or random mixing of plasma samples. The accuracy of the PAM-LIA was determined to be 94.7% through spiking recovery experiments, and the signal recovery after substance interference was 94–96%. The analyte showed 96% stability after six freeze-thaw cycles. The assay showed strong correlation with matched EDTA and serum samples, as well as matched EDTA and Li-Heparin samples. Additionally, a high correlation was observed between α-amidating activity and PAM-LIA. Finally, the PAM-LIA assay was successfully applied to a sub-cohort of a Swedish population-based study, comprising 4850 individuals, confirming its suitability for routine high throughput screening.
First Demonstration of In-Memory Computing Crossbar using Multi-level Cell FeFET
Authors: Soliman Taha , Chatterjee Swetaki , Laleni Nellie , Müller Franz , Kirchner Tobias , Wehn Norbert , Kämpfe Thomas , Chauhan Yogesh Singh , Amrouch Hussam 
Published: May 2023

Abstract
Advancements in AI led to the emergence of in-memory-computing architectures as a promising solution for the associated computing and memory challenges. This study introduces a novel in-memory-computing (IMC) crossbar macro utilizing a multi-level FeFET cell for multi-bit multiply and accumulate (MAC) operations. The proposed 1FeFET-1R cell design stores multi-bit information while minimizing device variabil21 ity effects on accuracy. Experimental validation was performed using 28 nm HKMG technology-based ferroelectric field-effect transistor (FeFET) devices. Unlike tradi23 tional resistive memory-based analog computing, our approach leverages the electrical characteristics of stored data within the memory cell to derive MAC operation results encoded in activation time and accumulated current. Remarkably, our design achieves 96.6%accuracy for handwriting recognition and 91.5%accuracy for image classification without extra training. Furthermore, it demonstrates exceptional performance, achiev28 ing 885.4 TOPS/W–nearly double that of existing designs. This study represents the first successful implementation of an in-memory macro using a multi-state FeFET cell for complete MAC operations, preserving crossbar density without additional struc31 tural overhead.
BAG3 as a novel prognostic biomarker in kidney renal clear cell carcinoma correlating with immune infiltrates
Authors: Gong Binghao , Huang Yuan , Wang Zhenting , Wan Bangbei , Zeng Yaohui , Lv Cai 
Published: May 2023

Abstract
Purpose BCL-2-associated athanogene 3 (BAG3) is an anti-apoptotic protein that plays an essential role in the onset and progression of multiple cancer types. However, the clinical significance of BAG3 in kidney renal clear cell carcinoma (KIRC) remains unclear.Methods Using Tumor IMmune Estimation Resource (TIMER), The Cancer Genome Atlas (TCGA), and Gene Expression Omnibus (GEO) database, we explored the expression, prognostic value, and clinical correlations of BAG3 in KIRC. In addition, Immunohistochemistry (IHC) of HKH cohort further validated the expression of BAG3 in KIRC and its impact on prognosis. Gene Set Cancer Analysis (GSCA) was utilized to scrutinize the prognostic value of BAG3 methylation. Gene ontology (GO) term analysis, Kyoto Encyclopedia of Genes and Genomes (KEGG), and Gene set enrichment analysis (GSEA) were used to identify potential biological functions of BAG3 in KIRC. Single-sample gene set enrichment analysis (ssGSEA) was performed to confirm the correlation between BAG3 expression and immune cell infiltration.Results BAG3 mRNA expression and protein expression were significantly downregulated in KIRC tissues compared to normal kidney tissues, associated with adverse clinical-pathological factors and poor clinical prognosis. Multivariate Cox regression analysis indicated that low expression of BAG3 was an independent prognostic factor in KIRC patients. GSEA analysis showed that BAG3 is mainly involved in DNA methylation and the immune-related pathways in KIRC. In addition, the expression of BAG3 is closely related to immune cell infiltration and immune cell marker set.Conclusion BAG3 might be a potential therapeutic target and valuable prognostic biomarker of KIRC and is closely related to immune cell infiltration.
A recessive LRR-RLK gene causes hybrid breakdown in cotton
Authors: Xu Peng , Xu Jianwen , Guo Qi , Xu Zhenzhen , Ji Wei , Yu Huan , Cai Jihong , Zhao Liang , Zhao Jun , Liu Jianguang , Chen Xianglong , Shen Xinlian 
Published: May 2023

Abstract
Key message An LRR-RLK gene causing interspecific hybrid breakdown between Gossypium. anomalum and G. hirsutum was identified by deploying a map-based cloning strategy.
The self-destructing symptoms of hybrid incompatibility in most cases are attributed to autoimmunity. The cloning of genes responsible for hybrid incompatibility in cotton is helpful to clarify the mechanisms underlying hybrid incompatibility and can break the barriers in distant hybridization. In this study, a temperature-dependent lethality was identified in CSSL11-9 (chromosome segment substitution line) with Gossypium anomalum chromosome segment on chromosome A11. Transcriptome analysis showed the differentially expressed genes related to autoimmune responses were highly enriched, suggesting that expression of CSSL11-9 plant lethal gene activated autoimmunity in the absence of any pathogen or external stimulus, inducing programmed cell death (PCD) and causing a lethal phenotype. The lethal phenotype was controlled by a pair of recessive genes, and then fine mapped between JAAS3191-JAAS3050 interval, which covered 63.87 kb in G. hirsutum genome and 98.66 kb in G. anomalum. We demonstrated that an LRR-RLK gene designated as hybrid breakdown 1 (GoanoHBD1) was the causal gene underlying this locus for interspecific hybrid incompatibility between G. anomalum and G. hirsutum. Silencing this LRR-RLK gene could restore CSSL11-9 plants from a lethal to a normal phenotype. Our findings provide new insights into reproductive isolation and may benefit cotton breeding.
Identification and Validation of Metabolism-Related Genes in Alzheimer’s Disease
Authors: Lian Piaopiao , Cai Xing , Wang Cailin , Liu Ke , Yang Xiaoman , Wu Yi , Zhang Zhaoyuan , Ma Zhuoran , Cao Xuebing , Xu Yan 
Published: May 2023

Abstract
Background：Due to its heterogeneity, the pathogenic mechanisms underlying Alzheimer's disease (AD) are not yet fully elucidated. Emerging evidence has demonstrated the critical role of metabolism in the pathophysiology of AD. This study explored the metabolism-related signature genes of AD and precisely identified AD molecular subclasses.
Methods: The AD datasets were obtained from the NCBI GEO, and metabolism-relevant genes were downloaded from a previously published compilation. Consensus clustering was utilized to identify AD subclasses. We evaluated the clinic characteristics, correlations with metabolic signatures and immune infiltration of the AD subclasses. Feature genes were screened by WGCNA and processed for GO and KEGG pathway analysis. Furthermore, we used three machine learning algorithms to further narrow down the selection of feature genes. Finally, we identified the diagnostic value and expression of feature genes using dataset and RT-PCR analysis.
Results: Three subclasses of AD were identified and designated as MCA, MCB, and MCC. MCA had high AD progression signatures and maybe a high-risk subgroup compared to the other two groups. MCA displayed high glycolysis, fructose and galactose metabolism, whereas citrate cycle and pyruvate metabolism were decreased, associating with high immune infiltration. Conversely, MCB is chiefly involved in the citrate cycle and exhibits elevated expression of immune checkpoint genes. Through WGCNA, a set of 101 metabolic genes were discovered to having the strongest association with the poor progression of AD. Ultimately, the application of machine learning algorithms enabled us to successfully pinpoint eight feature genes. Employing the nomogram based on the eight feature genes translates to distinct clinical benefits for the patients. As indicated by the datasets and RT-PCR analysis, these eight distinctive genes are intimately linked to the advancement of the AD.
Conclusion: Metabolic dysfunction is correlated with AD. Hypothetical molecular subclasses based on metabolic genes may provide new insights for individualized therapy of AD. The metabolic feature genes most robust correlation with the advancement of AD were GFAP, CYB5R3, DARS, KIAA0513, EZR, KCNC1, COLEC12 and TST.
Maternal and neonatal complications in pregnancy with COVID-19: a systematic review
Authors: Ibrahim Wisam Nabil , Al-Baker Fawziya , Awwad Sara , Abdallah Saja A , Al-Mazroea Abdulhadi H , Abdallah Atiyeh M , Al-Haidose Amal 
Published: May 2023

Abstract

The COVID-19 pandemic has had a profound impact on global health, leading to significant morbidity and mortality. Pregnancy can weaken the maternal immune response to the SARS-CoV-2 virus and increase the risk of severe adverse outcomes for both mother and fetus or newborn. Despite the well-known effects of viruses on pregnancy and the potential association with congenital anomalies, the impact of COVID-19 on pregnancy is still not fully understood. Here we systematically gathered and analyzed data from studies reporting the effects of maternal COVID-19 infection on mothers and their newborns. Through a comprehensive search of the PubMed, EMBASE, and Cochrane Library databases, 20 relevant studies were identified. Our analysis revealed that pregnant women with COVID-19 are at higher risk of morbidity and mortality and are more likely to require admission to the intensive care unit. Their newborns are also at increased risk of premature birth, low birth weight, and admission to neonatal intensive care unit. Our findings highlight the vulnerability of pregnant women and their newborns to COVID-19 complications and underscore the need for further research to better understand modes of neonatal SARS-CoV-2 transmission and the potential for congenital anomalies in early pregnancy infections.
Glucocorticoid-sparing effect of mepolizumab for severe eosinophilic granulomatosis with polyangiitis: a retrospective single-center observational study
Authors: Abe Yoshiyuki , Kogami Masahiro , Ando Taiki , Makiyama Ayako , Asanuma Yuko , Yamaji Ken , Tamura Naoto 
Published: May 2023

Abstract
Background
This study evaluated the glucocorticoid (GC)-sparing effect of mepolizumab (MEP) in severe eosinophilic granulomatous polyangiitis (EGPA).
Methods
Participants were patients diagnosed with EGPA according to Watts’ algorithm and who underwent outpatient care at Juntendo Hospital between November 2017 and January 2023. This longitudinal, observational study consisted of a retrospective analysis of medical records.
Results
Thirty-five patients were analyzed. The reasons for receiving MEP, by numbers of patients, were as follows: eosinophilia, 3; neuropathy exacerbation, 3; persistent neuropathy, 3; GC-sparing effect, 3; asthma exacerbation, 2; and skin ulcer exacerbation, 1. Compared with the MEP (−) group, the MEP (+) group had higher rates of remission and concomitant azathioprine treatment, and lower peripheral blood eosinophil count and GC dosage at last observation. The median GC dose after MEP was 2.8 (0.0–4.3) mg/day as prednisolone equivalent dose less than before.
Conclusions
The addition of MEP may be considered for patients with severe EGPA, with or without the use of azathioprine.
A real-world pharmacovigilance study of FDA Adverse Event Reporting System (FAERS) events forpaxlovid and molnupiravir
Authors: Yuan Chao , Yin Guisen , Song Guiling , Guan Ying 
Published: May 2023

Abstract
Questions have been raised about the safety of paxlovid and molnupiravir as antiviral drugs for the treatment of COVID-19 since the pandemic. We applied t he FDA Adverse Event Reporting System (FAERS) to assess the safety by performing a disproportionality analysis to identify potential risks of paxlovid and molnupiravir. The number of paxlovid signals was approximately 11 times higher than that of molnupiravir, with most signals of these two drugs overlapped. General disorders and administration site conditions (ROR: 0.52, 95% CI: 0.58- 2.18), infections and infestations (ROR: 0.18, 95% CI: 0.23-6.64), nervous system disorders (ROR: 1.41, 95% CI: 0.79-1.58) were the top 3 signals for paxlovid, with gastrointestinal disorders (ROR: 4.13, 95% CI: 0.27-4.54), skin and subcutaneous tissue disorders (ROR: 11.51, 95% CI: 0.10-12.92), nervous system disorders (ROR: 1.41, 95% CI: 0.79-1.58) for molnupiravir. Paxlovid-induced infections, skin and subcutaneous tissue disorders, and molnupiravir-induced musculoskeletal and connective tissue disorders, as well as potential safety signals on the heart, eyes and ears needlong-term observation, especially for signals not included in the instructions. The adverse events on this study confirms most of the instructional information for paxlovid and molnupiravir, both drugs need to be monitored for risk signals such as acute respiratory failure, hematologic and lymphatic system.
Optimisation of Warfarin-Dosing Algorithms for Han Chinese Patients with CYP2C9*13 Variants
Authors: Wang Dongxu , Wu Hualan , Zhang Qing , Zhou Xiaoyue , An Yang , Zhao Anxu , Chong Jia , Wang Shuanghu , Wang Fang , Yang Jiefu , Dai Dapeng , Chen Hao 
Published: May 2023

Abstract
Background Existing pharmacogenetic algorithms cannot fully explain warfarin dose variability in patients. CYP2C9*13 is an important allelic variant in the Han Chinese population. However, adjustment of warfarin dosing in CYP2C9*13 variant carriers remains unclear. To the bets of our knowledge, this study is the first to assess the effects of adjusting warfarin dosages in Han Chinese patients harbouring CYP2C9*13 variants.Methods In total, 971 warfarin-treated Han Chinese patients with atrial fibrillation were enrolled in this study. Clinical data were collected, and CYP2C9*2, *3, *13 and VKORC1-1639 G > A variants were genotyped. We quantitatively analysed the effect of CYP2C9*13 on warfarin maintenance dose and provided multiplicative adjustments for CYP2C9*13 using validated pharmacogenetic algorithms.Results Approximately 0.6% of the Han Chinese population carried CYP2C9*13 variant, and the genotype frequency was between those of CYP2C9*2 and CYP2C9*3. The warfarin maintenance doses were significantly reduced in CYP2C9*13 carriers. When CYP2C9*13 variants were not considered, the pharmacogenetic algorithms overestimated warfarin maintenance doses by 1.03–1.16 mg/d on average. The actual warfarin dose in CYP2C9*13 variant carriers was approximately 40% lower than the algorithm-predicted dose. Adjusting the warfarin-dosing algorithm according to the CYP2C9*13 allele could reduce the dose prediction error.Conclusion Our study showed that the algorithm predicted doses should be lowered for CYP2C9*13 carriers. Inclusion of the CYP2C9*13 variant in the warfarin-dosing algorithm tends to predict the warfarin maintenance dose more accurately and improves the efficacy and safety of warfarin administration in Han Chinese patients.
CST6 promotes the aggressive phenotype of thyroid cancer through maintaining autophagy
Authors: Ren Hao , Zhang Qi , Bao Lisha , Lu Xixuan , Zhu Chaozhuang , Li Ying , Che Yulu , Huang Ping , Ge Jingyan , Ge Minghua 
Published: May 2023

Abstract
Thyroid Cancer (TC) is the most common malignant tumor of the endocrine system. Cystatin M/E (CST6) has been demonstrated to have a multifaceted role in several types of cancers. However, its potential mechanisms in the progression of TC have not been fully identified. Our results revealed that CST6 expressions were upregulated in TC tissues and cells compared with normal thyroid tissues and cells. High expression of CST6 was negatively correlated with poor prognosis of thyroid cancer patients. Functional assays showed that CST6 promoted TC cells malignant phenotype, including proliferation, colony formation, migration, and invasion. Inhibition of CST6 remarkably alleviated tumor growth and metastasis of ATC xenografts in nude mouse and zebrafish model. CST6 showed interaction with cathepsin B (CTSB). CTSB knockdown profoundly inhibited the aggressive behavior of TC cells. Of note, loss of CST6 attenuated the activity of CTSB, which led to the decrease of autophagy progress. Collectively, our findings demonstrated an essential role of CST6 in the development of TC through CTSB-mediated autophagy.
Assessment of Rainfall-runoff Relation/mechanisms Under Different Land Uses and Landscape Position in North Western Ethiopia
Authors: Tamer Mulugeta , Adego Enyew , Tsegaye Derege , Haregewoyen Nigussie , Abiyu Abrham 
Published: May 2023

Abstract
Purpose:
This research aimed to develop rainfall-runoff relations for different land use types, to establish a relationship between runoff and soil loss under different land use and soil moisture conditions, and to evaluate the variation of soil water content in different depths of soil and land use types.
Methods:
An automatic and manual rain gauge that was installed in the watershed at the nearby testing plots was used to record the rainfall data. Three separate plots with various land uses and slope positions were used to gather runoff and sediment loss. Every morning -at 6:30 a.m., runoff and sediment data were collected from the runoff plots following each rainstorm occurrence.
Result:
The rainfall-runoff relations on all land use types were significant at (P<0.001). The infiltration rate and rainfall intensity analysis show that the dominant runoff generation mechanism of the watershed was saturation excess. The ECH2O Ec-5 soil moisture sensor probes show that the volumetric soil moisture content increased with increasing soil depth on grazing land, while the reverse happened on the cultivated land. The volumetric moisture content on both land use types had very high statistical significance among the 5 depths of the soil (P<0.001).
Conclusion:
Steep slope areas should be closed and there should be some afforestation program in the area. Free grazing grasslands should be changed to closed grazing land use systems.