bluuebunny/crossref_metadata_2025_split huggingface dataset:
CEBPA enhances tumor radiosensitivity through ferroptosis and associates with tumor immune microenvironment
Authors: Du Yimeng , Liu Ji , Han Yuchen , Li Hui , Kang Xiaofeng , Zhu Junwen , Li Xingyu , Wu Di , Li Wenchao , Ma Jianli , yan zhifan 
Published: May 2023

Abstract
Radiotherapy has shown measurable efficacy with objective response rate and prolonged progression-free survival (PFS) in patients with breast cancer (BC). However, radioresistance is still a major challenge in BC radiotherapy, leading to limited clinical application and treatment failure. Therefore, elucidating the underlying mechanisms and developing effective strategies to enhance the radiosensitivity is of clinical importance. In the present study, we demonstratedCCAAT enhancer-binding protein alpha (CEBPA) as a specific marker for predicting the efficacy of radiotherapy in BC patients. CEBPA enhanced radiotherapeutic sensitivity of BC by increasing the apoptosis both in vitro and in vivo. Mechanistically, CEBPA executed radio-sensitizing function in BC by promoting ferroptosis through negatively regulating the nuclear factor E2-related factor 2 (Nrf2) pathway genes. Furthermore, high expression of CEBPA was positively correlated with the infiltration of cytotoxic immune cells in BC microenvironment. In summary, our work revealed CEBPA as a critical regulator to enhance radiosensitivity, induce ferroptosis, as well as modulate tumor immune microenvironment in BC. Induction of CEBPA or ferroptosis with specific inducers or modulation of tumor immune microenvironment would be potential sensitizing therapeutic strategies in BC.
A novel MT-ATP6 variant associated with complicated ataxia in two unrelated Italian patients: case report and functional studies.
Authors: Sala Daniele , Marchet Silvia , Nanetti Lorenzo , Legati Andrea , Mariotti Caterina , Lamantea Eleonora , Ghezzi Daniele , Catania Alessia , Lamperti Costanza 
Published: May 2023

Abstract
Background: The MT-ATP6 gene is a mitochondrial gene which encodes for the intramembrane subunit 6 (or A) of the mitochondrial ATP synthase. The latter corresponds to mitochondrial complex V, which intervenes in the last step of oxidative phosphorylation to produce cellular ATP through aerobic metabolism. Although classically associated with the NARP syndrome, recent evidences point out to an important role of MT-ATP6 pathogenic variants in complicated adult onset ataxias.
Methods: We described two unrelated patients with adult onset cerebellar ataxia associated with severe optic atrophy and mild cognitive impairment. We performed the sequencing of the whole mitochondrial DNA. We employed patients’ primary fibroblasts and cytoplasmic hybrids (cybrids), generated from patients-derived cells, to assess the activity of respiratory chain, oxygen consumption rate (OCR), ATP production and mitochondrial membrane potential.
Results: In both patients, we identified the same novel m.8777T>C variant in MT-ATP6 with variable degree of heteroplasmy in different tissues. We identify an additional novel variant in MT-ATP6, m.8879G>T, with high percentage of heteroplasmy, in the patients with the most severe phenotype. A significant reduction in complex V activity, OCR and ATP production was observed in cybrid clones homoplasmic for the m.8777T>C variant, while no such defects were detected in m.8879G>T homoplasmic clones. In addition, fibroblasts with high heteroplasmic levels for the m.8777T>C variant showed increased polarization of mitochondrial membranes.
Conclusions: We describe a new pathogenic mtDNA variant in MT-ATP6 associated with adult onset ataxia, supporting the value of mtDNA screening within the diagnostic workflow of selected patients with late onset ataxias.
Global DNA methylation and telomere length as markers of accelerated ageing in non-alcoholic fatty liver disease patients with HIV infection
Authors: Moreno Elena , Martínez-Sanz Javier , Martín-Mateos Rosa , Díaz-Álvarez Jorge , Serrano-Villar Sergio , Burgos-Santamaría Diego , Luna Laura , Vivancos María Jesús , Moreno-Zamora Ana , Pérez-Elías María Jesús , Moreno Santiago , Dronda Fernando , Montes María Luisa , Sánchez-Conde Matilde 
Published: May 2023

Abstract
Metabolic-dysfunction-associated fatty liver disease (MAFLD) is a comorbidity that generally increases in people living with HIV (PLWH). This condition is usually accompanied by persistent inflammation and premature immune system ageing. In this prospective cohort study, we describe a straightforward methodology for quantifying the effect of HIV on aging factors compared with other relevant conditions, such as MAFLD, by quantifying DNA methylation and telomere length. Fifty-seven samples in total, thirty-eight from HIV-infected and nineteen from HIV-uninfected participants with or without MAFLD, were obtained and subjected to DNA extraction from peripheral blood mononuclear cells (PBMCs). DNA methylation and telomere length quantification were performed using an adapted enzyme-linked immunosorbent assay (ELISA) and qPCR, respectively. The quantification results were analysed and corrected by clinically relevant variables in this context, such as age, sex, and metabolic syndrome. The results showed an increased association with these age-acceleration markers in individuals with HIV, who were also diagnosed with MAFLD. This highlights the importance of HIV infection and MAFLD conditions in the biological ageing process in PLWH, and the fact that these markers of ageing can be measured through DNA methylation and telomere length quantification. Thus, we propose including these quantifications in studies of comorbidities to better understand them and potentially prevent their effects in this population.
Protective efficacy of Nigella Sativa oil, Vitamin C and E against Aflatoxin M1 (AFM1) induced Aflatoxicoses in neonatal Wister rats
Authors: Badmos Amina , Oni Eniola , Adeleye Tolulope , Aladesida Adeyinka , Oluwafemi Flora 
Published: May 2023

Abstract
This study investigated the possible protective role of Nigella sativa oil (NSO), vitamin C, and E in one hundred and seventeen (117) aflatoxicoses induced neonatal wistar rats with Aflatoxin M1(AFM1), randomly divided into 13 groups of nine rats each (A-M) for 12 weeks. Group A served as control, while groups B, C, D, E received (9ng/l AFM1, 9ng/l AFM1 + Vit.C, +Vit.E, +NSO), groups F, G, H, I had (235 ng/l AFM1, 235 ng/l AFM1 + Vit.C, +Vit.E, +NSO) and groups J, K,L,M received (456ng/l AFM1, 456ng/l AFM1 + Vit.C, +Vit. E, + NSO). Rats were fasted overnight and anesthetized, cardiac blood was collected for the analysis of Packed Cell Volume (PCV), Haemoglobins (HB), Red Blood Cells (RBC), White Blood Cells (WBC), and levels of Aspartate aminotransferase (AST), Alanine aminotransferase (ALT), and Alkaline Phosphatase (ALP) The liver tissues were examined for histopathological changes. AFM1 concentrations significantly decreased blood levels and liver enzymes. Simultaneous supplementation with Nigella sativa oil, vitamin C, and E showed a significant increase in the PCV, HB, and RBC and a decrease in the WBC as well as the liver enzymes (ALT, ALP, and AST). NSO treatment restored blood loss and repaired damaged liver tissues irrespective of the AFM1 concentration administered, vitamin C and E restored changes only at low AFM1 concentrations.
Androgen receptor and osteoglycin gene expression predicting prognosis of metastatic prostate cancer
Authors: Kameda Tomohiro , Sugihara Toru , Obinata Daisuke , Oshima Masashi , Yamada Yuta , Kimura Naoki , Takayama Kenichi , Inoue Satoshi , Takahashi Satoru , Fujimura Tetsuya 
Published: May 2023

Abstract

This study aimed to identify the predictive factors associated with oncological outcomes in metastatic hormone-sensitive prostate cancer-related genes. A nomogram for predicting prostate cancer-specific survival (CSS) was constructed based on biopsy samples from 103 patients with metastatic hormone-sensitive prostate cancer. We analyzed the association between clinical data and mRNA expression levels. The nomogram was externally validated in another cohort (n = 50) using a concordance index. Based on the cutoff value, determined by a receiver operating characteristic analysis, longer CSS was observed in the high osteoglycin and androgen receptor expression level groups (> 1.133 and > 0.00; median CSS, 85.3 vs. 52.7 months, p = 0.082, and 69.1 vs. 32.1 months, p = 0.034, respectively), compared with that of the low expression level groups. The nomogram predicting CSS included hemoglobin (≥ 13.7 g/dL or < 13.7 g/dL), serum albumin (≥ 3.1 g/dL or < 3.1 g/dL), serum lactate dehydrogenase (≥ 222 IU/L or < 222 IU/L), total Japan Cancer of the Prostate Risk Assessment score, androgen receptor expression level, and osteoglycin expression level. The concordance indices for internal and external validations were 0.664 and 0.798, respectively. A nomogram that integrates expression levels of androgen receptors and osteoglycin to predict CSS in metastatic hormone-sensitive prostate cancer was established.
Effects of the Addition of Polyvinyl Pyrrolidone (PVP) as a Corrosion Inhibitor to Zr/V-based Conversion Coating on Aluminum Alloy
Authors: Cui Zhichun , Li Jianzhong , Zhang Zhiying , Yang Wenhu , Qiu Donghai 
Published: May 2023

Abstract

Polyvinyl pyrrolidone (PVP) was selected as an additive for zirconium-based conversion treatment on aluminum alloy substrate. Scanning electron microscopy (SEM), energy dispersive spectroscopy (EDS) and X-ray photoelectron spectroscopy (XPS) were used to investigate the effect of PVP on the morphology and composition of the conversion coating. Neutral salt spray (NSS), electrochemical impedance spectroscopy (EIS), and Tafel polarization tests were used to characterize the corrosion resistance of the conversion coating. The results show that the conversion coating was mainly composed of metal oxide (ZrO2, VO2, V2O5, etc.), metal fluoride (ZrF4, Na3AlF6) and PVP. The density of Na3AlF6 crystal is increased by adding PVP into the conversion bath. This is due to the adsorption of PVP molecules on the surface of the substrate, which trap metal ions and thus facilitate the coating formation reaction. The corrosion resistance of the substrate treated by conversion coating containing PVP was significantly improved. When the amount of PVP introduced into the conversion bath is 1.5 g/L, the conversion coating can provide excellent corrosion resistance to the substrate. In addition, the non-polar groups in the PVP molecule can effectively improve the adhesion between the conversion coating and the electrophoretic coating.
An Empirical Study on the Dynamic Evolution of Health Production Efficiency and Its Influencing Factors in China——Based on Stochastic Frontier Analysis (SFA)
Authors: Jiali Yu , Shangguang Yang , Mengfan Xia 
Published: May 2023

Abstract
Exploring the evolution law of health production efficiency and its influencing factors in China is conducive to maximizing the promotion of health output under the constraints of limited resources, and is useful for alleviating medical resource shortages and solving the problems of expensive and difficult medical treatment and medical treatment for the public. The stochastic frontier production function is used to calculate the health production efficiency in China and discuss its evolution law using panel data from 31 provinces in China from 2002 to 2018. At the same time, the factors that affect the health production efficiency are empirically tested. The study discovered that China's regional health production efficiency exhibits a spiral rise—a relatively stable evolution trend, and overall efficiency performance is acceptable. There are significant spatial differences in regional health production efficiency. The efficiency gap between the country and the three traditional economic zones tends to narrow. In addition, with the exception of Northwest China, the efficiency of other comprehensive economic zones has absolute convergence characteristics. The level of urbanization, the degree of government attention, the reform of the medical and health system, and population density all have a positive impact on health production efficiency. Medical insurance coverage has not passed the significance test. Despite unfavorable factors such as current exam-oriented education and the continuous expansion of college enrollment, the increase in the number of years of education received by residents did not promote, but rather hampered, the efficiency of regional health production.
Relationship between omentin-1 levels in patients with coronary artery disease and healthy controls: A systematic review and meta-analysis
Authors: Tongxia WANG , CHEN Zhangrong 
Published: May 2023

Abstract
Background Omentin-1 is associated with diseases such as obesity, hypertension, diabetes, and coronary heart disease, as well as a range of metabolisms. This paper aims to describe the role of omentin-1 in patients with coronary artery disease (CAD).Method We performed a systematic review and meta-analysis of omentin-1 levels in patients with CAD compared to those in healthy individuals. We searched PubMed, Embase, and Cochrane Library from database inception to July 2022. The data extracted included the author, publication year, country, study design, sample size, sex, age, body mass index, sample source, and outcomes including levels of omentin-1 and their relationship with CAD. All data were analyzed using STATA Statistical Software, version 15.1 (StataCorp, College Station, TX, USA). For continuous variables, standardized mean difference (SMD), and mean difference (95% confidence interval [CI]) the combined effect size was used, and for risk factor assessment, the odds ratio (OR; 95% CI) was used. Both I2 and Q tests were used to evaluate heterogeneity.Results Twelve case-control studies were included in the meta-analysis. The sample size of the patients was 2034, including 1205 patients with CAD and 829 healthy controls. There were 10 studies that compared omentin-1 levels in patients with CAD to those in healthy people. The pooled results showed that the level of omentin-1 in patients with CAD was significantly lower than that in healthy people (SMD = -1.56, 95% CI: -2.00 to -1.13, P = 0.000). Five studies reported an association between omentin-1 levels and CAD. The pooled results suggested that elevated omentin-1 levels were associated with reduced incidence of CAD (OR = 0.95, 95% CI: 0.91 to 0.99).Conclusion The pooled results showed that the level of omentin-1 in patients with CAD was significantly lower than that in healthy people and suggested that elevated omentin-1 levels are associated with reduced incidence of CAD.
Comparing the knowledge and management of hypertensive individuals
Authors: Simamane Mandisa , Watson Estelle , Constantinou Demitri , Gradidge Philippe Jean-Luc 
Published: May 2023

Abstract

Background The high prevalence of hypertension in South Africa can be attributed to poor community assessment, limited protocols for treatment and management of the disease and the relatively asymptomatic characteristic of the disease.Aim To determine the knowledge and management of hypertension in private and public health care sectors in an African population.Method One hundred and thirty-seven sample population was taken from black ethnic community. The cross-sectional study was used as a convenience sampling of participants. Interview-led questionnaires were also used to assess participants’ demographic information, hypertension management and lifestyle factors.Results Data analysis yielded the group’s overall blood pressure 124.7/79.6mm Hg with public health care sector (125.9/80.3mm Hg) and the private health care sector (123.3/78.8mm Hg) (p = 0.4). The participants showed significant differences within the groups on all the ‘Medication and adherence’ and ‘Management of hypertension’ categories.Conclusion Both the participants of the private health care sector and public health care sector had the same understanding of the knowledge and management of hypertension, as well as its risk factors.Contribution: The objectives of the study were met through the clinical application understanding the knowledge and management of hypertension and its risk factors to help identify the differences between the patients utilising public and private health care providers.
First record of the African species Leptodelphax maculigera (Stål, 1859) (Hemiptera: Delphacidae) in Brazil
Authors: Ferreira Kerolainy R. , Bartlett Charles R. , Asche Manfred , Silva Liz R. S. , Magalhães Vinícius S. , Gondinho Karina C. Albernaz 
Published: May 2023

Abstract
This study reports the first occurrence of Leptodelphax maculigera (Stål, 1859) in Brazil and the Americas. Until now, this species has not been reported outside of Africa. The notification occurred in the State of Goiás in species of agronomic importance, as corn, Brachiaria, elephant grass, cultivar BRS Capiaçu and beans. The identification of the species was carried out through the morphological analysis of the male terminalia. This species has been found in corn plants, were sharing the same space with another wellknown species great economic importance for the culture, the Dalbulus maidis (DeLong & Wolcott) and espite the morphological differences between the species, it's possible these two may be evaluated in the field only as “corn leaf-hoppers”, without distinguishing the species because the presence of L. maculigera in Brazil was unknown. Furthermore, another worrying factor is the ability of the genus Leptodelphax to transmit phytoplasma as reported the literature, which may potentiate the damage to possible host plants in Brazil. Thus, the confirmed presence of L. maculigera in Brazil and the morphological distinction of the two species are key factors for establishing Integrated Pest Management (IPM) in the areas where it is found.
Explosive colonic perforation, a catastrophic complication of rectal Argon Plasma Coagulation therapy
Authors: Cullen Fraser , Ramsay George , Walker Kenneth G 
Published: May 2023

Abstract
Argon plasma coagulation (APC) is a widely used technique which uses a jet of ionised argon gas to help control gastrointestinal bleeding. A 65-year-old gentleman underwent APC for radiation induced proctitis. On ignition of the argon laser, a large “popping” noise was heard and he experienced immediate abdominal pain. He had suffered a large sigmoid perforation that required a Hartmann’s procedure. Spontaneous colonic explosion is a known, but very rare, complication of APC. It has been suggested this is due to high intraluminal levels of combustible gases such as hydrogen or methane. Levels of these are thought to be reduced by full bowel preparation. Oxygen is also required for combustion, levels of which can be reduced by using carbon dioxide as the insufflated gas during the colonoscopy, rather than room air. Use of both full bowel preparation and C02 insufflation could help prevent this rare complication from occurring in the future.
The relationship between overweight/obesity and oral health in children
Authors: Yoldaş Meyri , Vural Simge , BOLU SEMİH , DANIŞ AYŞEGÜL 
Published: May 2023

Abstract
Aim
The purpose of this study was to evaluate the effects of obesity and overweight on oral/dental health and blood biochemistry parameters in children. Eighty-seven children (29 boys and 58 girls) aged 1-18 presenting to in our pediatric clinic were included in the study.
Methods
The patient group consisted of overweight or obese children, and the control group of normal weight individuals. Pediatric patients were examined simultaneously by a pediatrician and a pediatric dentist. Oral/dental health examinations were performed by a pediatric dentist, and DMFT/dmft index, CPITN index and dental plaque calculations were made. In addition, daily tooth brushing habits were evaluated by questioning the time of the last dental examination. Oral and dental health examination findings and blood biochemistry parameters were compared between the two groups.
Results
Obesity/overweight was found to be associated with, the dental plaque index, DMFT/dmft index, and elevation in the blood biochemistry parameters CRP and PTH among the children in this study.
Conclusion
The observation of significant elevation in DMFT and dental plaque indices, and numbers of filled deciduous teeth showed that oral/dental health problems and dental decay may emerge more frequently in obesity/overweight children.
A Newly Established Cuproptosis-Related Long Noncoding RNA Signature for Predicting Prognosis and the Immune Microenvironment in Stomach Adenocarcinoma
Authors: Xiao Yaodong , Li Bowen , Shi Yiheng , Zhang Cheng , Fei Sujuan , Zhao Yanchao , Li Li 
Published: May 2023

Abstract
Cuproptosis is a form of cell death that is considered as a new direction for future cancer therapy. It has been less reported in stomach adenocarcinoma (STAD). Download transcriptomic data, genomic mutation data and clinical data for STAD from the TCGA website. We identified 430 cuproptosis-related long noncoding RNA (LncRNAs). Cox models screened 8 cuproptosis-related lncRNAs (AC005050.3, AC016394.2, AL606970.1, BX890604.1, C5orf66, HAGLR, LINC01094, TDRKH- AS1), and the risk model were constructed. Based on the median risk score, STAD patients were divided into a high-risk group and a low-risk group. kaplan-Meier survival curves showed that the low-risk group had a higher survival rate than the high-risk group. Operating characteristic curve and principal component analysis showed that the model had better predictive power. The tumor mutational burden was higher in the low-risk group, suggesting greater sensitivity to immunotherapy, and subsequently screening for sensitive drugs in different risk groups. Finally, qPT-PCR results showed that eight lncRNAs were highly expressed in STAD cells. In conclusion, these 8 LncRNAs may be potential biomarkers for predicting the prognosis of STAD patients and assessing the effect of immunotherapy and drug sensitivity in the future.
Molecular characterisation of human JC polyomavirus and risk of developing prostate adenocarcinoma at an early age.
Authors: TIABI Ikram , ENNAJI Youssef , Abumsimir Berjas , Sosse Soukayna Alaoui , Saif Imane , Tadlaoui Kaoutar Anouar , Takati Nadia , Laraqui Abdelilah , Ennibi Khalid , Mrabti Mohammed , Benchekroun Mohammed Nabil , Ennaji Moulay Mustapha 
Published: May 2023

Abstract
Background: The putative functions of JC polyomavirus (JC PyV) infections and Simian Virus 40 (SV40) in the initiation of prostate tumors have recently been discovered.
Objective: The objective of our study is to determine the frequency and the genotype of JCPyV and SV40 infections and the clinical correlation of tumor characteristics.
Material and methods: 80 samples were analyzed 50 prostate cancer patients and 30 control cases. DNA extracts were obtained from 50 men treated at the University Hospital of the city of Rabat (Morocco) between June 2021 and February 2022. These samples were then genotyped and analyzed using Sanger.
Results: Ten JC PyV infections were detected, and 50% of patients had a Gleason score of 6. A significant association between the following variables was noted: age at diagnosis and JC PyV infection (P=0.004); background medical (P=0.034); alcohol (P=0.035); BMI (P<0.001). However, no significant differences were observed between men infected and uninfected concerning other settings pathological, including pathological Gleason score, antigen prostate-specific (PSA), history surgical, stage histology of the tumor (P =0.172, P=0.107, P=1.00, P=0.258, and P=0.884, respectively). So, we don't have could detect SV40 infection in PCa samples, suggesting this virus is unrelated to the development of this type of tumor. The evolution structure of the JC PyV according to the sequences previously aligned was discussed.
Conclusion: Our results reveal the correlations between the parameters of the Clinico-pathology of prostate tumors and JC PyV infections. Research prospects with a wide size sample are essential to elucidate statistically the relationship between viral infections and parameters of prostate tumors.