bluuebunny/crossref_metadata_2025_split huggingface dataset:
Morphological changes and twoNodalparalogs drive left-right asymmetry in the squamate veiled chameleon (C. calyptratus)
Authors: Shylo Natalia A. , Smith Sarah E. , Price Andrew , Guo Fengli , McClain Melainia , Trainor Paul 
Published: January 2023

ABSTRACTThe ancestral mode of left-right (L-R) patterning involves cilia in the L-R organizer. However, the mechanisms regulating L-R patterning in non-avian reptiles remains an enigma, since most squamate embryos are undergoing organogenesis at oviposition. In contrast, veiled chameleon (Chamaeleo calyptratus) embryos are pre-gastrula at oviposition, making them an excellent organism for studying L-R patterning evolution. Here we show that veiled chameleon embryos lack motile cilia in their L-R organizer, consistent with the loss of motile cilia being a synapomorphy of all reptiles. Furthermore, in contrast to avians, geckos and turtles, which have oneNodalgene, veiled chameleon exhibits expression of two paralogs ofNodalin the left lateral plate mesoderm, albeit in non-identical patterns. Using live imaging, we observed asymmetric morphological changes that precede, and likely trigger, asymmetric expression of the Nodal cascade. Thus, veiled chameleons are a new and unique model for studying the evolution of L-R patterning.
Natural Killer cell contractility and cytotoxicity is driven by TLR3 agonist-mediated TAZ cytoplasmic sequestration
Authors: Pei Wong Darren Chen , Xia Zekun , Shao Nandi , Yeo Jin Ye , Yow Ivan , Thivakar T. , Salazar Andres M. , Liou Yih-Cherng , Low Boon Chuan , Ding Jeak Ling 
Published: January 2023

AbstractThe evolutionarily conserved YAP/TAZ mechano-responsive transcription cofactors regulate development and tumorigenesis. Here, we show for the first time that human natural killer (NK) cells specifically express TAZ but not YAP, and TAZ serves to limit NK cytotoxicity. The TLR3 agonist, hiltonol, was able to trigger cytoplasmic sequestration of TAZ, which increased NK cytotoxicity. Mechanistically, a low dose of hiltonol enhanced the intracellular contractility of NK cells accompanied by an increase in active RhoA and myosin light chain phosphorylation, conceivably through an increase in ERK1/2 activation-dependent ROS production. Importantly, we showed that the dissociation of LATS1 from actin upon activation of contractility, is required to sequester TAZ in the cytoplasm. Functionally, hiltonol also reduced the NK cell surface inhibitory receptors, KIR3DL1 through c-Myc inhibition and PD-1 through enhanced contractility. Direct inhibition of c-Myc promoted NK cytotoxicity against K562 lymphoblast. We corroborated our findings by coculturing hiltonol-pretreated NK cells with breast and lung cancer cells, and demonstrated increased NK-mediated cancer killing, which conceivably occurred via sequestration of TAZ to the cytoplasm which facilitated NK cytotoxicity. Our findings pave the way forex vivorejuvenation of NK cells forin vivoimmunotherapies, which could involve a cocktail of hiltonol and c-Myc inhibitor.
Digital audience engagement in Nigeria: a shift from live to online audience experience in Tosin Jobi-Tume’s Corona Palava
Authors: Mark Tekena 
Published: January 2023

PurposeArts practitioners have looked for ways to engage their audiences and sustain their interests and patronage of theatre shows amidst the coronavirus pandemic that kept patrons at home. This has led to digital engagement with audience members via social media. This research paper looks at digital audience engagement in Nigeria’s theatre using Segun Adefila’s production of Tosin Jobi-Tume’s Corona Palava which was performed on 4 August, 2020, at the Crown Arts Centre in Bariga, Lagos, Nigeria and later made available on Facebook on 22 December, 2020 and 21 December 21 2021.Design/methodology/approachThis study is based on the “Arts Audience Experience Index” theory proposed by Radbourne et al. (2009). It employs netnography as its methodology. This entails observing and analysing users’ comments, communication style, frequency of engagement and dwell time while watching Corona Palava production on the researcher’s Facebook timeline “Tekena Gasper Mark” and on the Facebook group “Bolt Drivers in Port Harcourt”. Overall, 53 comments (39 from the researcher’s Facebook friends and 14 from members of the Facebook group “Bolt Drivers in Port Harcourt”) were sampled and analysed to provide insights into how the spectators experienced the Corona Palava production.FindingsThe text that accompanied the Facebook video provided viewers with information about the performance, helped them prepare for what to expect, reduced the likelihood that they would experience any unease while watching it and increased the likelihood that they would look for similar performances in the future. They were pleased with the performance; there were no functional risks, no economic risks and no psychological and social risks. Although they may have watched it at varying times, Facebook provided a space for them to engage with the performance as a group and share their thoughts in the post-performance comments.Research limitations/implicationsOne of the study’s limitations is that one cannot ascertain how many of the respondents are drivers. Also, the researcher believes that the length of the video may have discouraged participation in the study. In order to increase viewership and provide better findings, future studies and artistic endeavours could consider shorter pieces (about 3–5 min) and wider locations (transportation businesses) where a larger number of drivers with active social media presence, can participate in the research.Practical implicationsThis study documents an innovative approach to reaching theatre audience via social media in Nigeria.Social implicationsThis research demonstrates that Nigerian theatre and arts practitioners are reinventing their approaches to play production by using the social media to reach their audiences in the post-COVID-19 era.Originality/valueThe study reveals that as a result of the coronavirus pandemic, Nigerian theatre artists have looked for ways to engage their audiences and sustain their interest and patronage of arts projects through social media.
Students' adoption of AI-based teacher-bots (T-bots) for learning in higher education
Authors: Pillai Rajasshrie , Sivathanu Brijesh , Metri Bhimaraya , Kaushik Neeraj 
Published: January 2023

PurposeThe purpose of this paper is to investigate students' adoption intention (ADI) and actual usage (ATU) of artificial intelligence (AI)-based teacher bots (T-bots) for learning using technology adoption model (TAM) and context-specific variables.Design/methodology/approachA mixed-method design is used wherein the quantitative and qualitative approaches were used to explore the adoption of T-bots for learning. Overall, 45 principals/directors/deans/professors were interviewed and NVivo 8.0 was used for interview data analysis. Overall, 1,380 students of higher education institutes were surveyed, and the collected data was analyzed using the Partial Least Squares Structural Equation Modeling (PLS-SEM) technique.FindingsThe T-bot's ADI’s antecedents found were perceived ease of use, perceived usefulness, personalization, interactivity, perceived trust, anthropomorphism and perceived intelligence. The ADI influences the ATU of T-bots, and its relationship is negatively moderated by stickiness to learn from human teachers in the classroom. It comprehends the insights of senior authorities of the higher education institutions in India toward the adoption of T-bots.Practical implicationsThe research provides distinctive insights for principals, directors and professors in higher education institutes to understand the factors affecting the students' behavioral intention and use of T-bots. The developers and designers of T-bots need to ensure that T-bots are more interactive, provide personalized information to students and ensure the anthropomorphic characteristics of T-bots. The education policymakers can also comprehend the factors of T-bot adoption for developing the policies related to T-bots and their implications in education.Originality/valueT-bot is a new disruptive technology in the education sector, and this is the first step in exploring the adoption factors. The TAM model is extended with context-specific factors related to T-bot technology to offer a comprehensive explanatory power to the proposed model. The research outcome provides the unique antecedents of the adoption of T-bots.
Remote integration of advanced manufacturing technologies into production systems: integration processes, key challenges and mitigation actions
Authors: Fattouh Anas , Chirumalla Koteshwar , Ahlskog Mats , Behnam Moris , Hatvani Leo , Bruch Jessica 
Published: January 2023

PurposeThe study examines the remote integration process of advanced manufacturing technology (AMT) into the production system and identifies key challenges and mitigating actions for a smoother introduction and integration process.Design/methodology/approachThe study adopts a case study approach to a cyber-physical production system at an industrial technology center using a mobile robot as an AMT.FindingsBy applying the plug-and-produce concept, the study exemplifies an AMT's remote integration process into a cyber-physical production system in nine steps. Eleven key challenges and twelve mitigation actions for remote integration are described based on technology–organization–environment theory. Finally, a remote integration framework is proposed to facilitate AMT integration into production systems.Practical implicationsThe study presents results purely from a practical perspective, which could reduce dilemmas in early decision-making related to smart production. The proposed framework can improve flexibility and decrease the time needed to configure new AMTs in existing production systems.Originality/valueThe area of remote integration for AMT has not been addressed in depth before. The consequences of lacking in-depth studies for remote integration imply that current implementation processes do not match the needs and the existing situation in the industry and often underestimate the complexity of considering both technological and organizational issues. The new integrated framework can already be deployed by industry professionals in their efforts to integrate new technologies with shorter time to volume and increased quality but also as a means for training employees in critical competencies required for remote integration.
Effect of business intelligence on operational performance: the mediating role of supply chain ambidexterity
Authors: Mbima Daniel , Tetteh Francis Kamewor 
Published: January 2023

PurposeThis study aims to examined the impact of business intelligence (BI) and supply chain ambidexterity (SCA) on operational performance (OP), contributing to dwarf knowledge in small- and medium-sized enterprises (SMEs) in the context of emerging economies. The mediating role of SCA was considered in the proposed model.Design/methodology/approachThe study used the quantitative method to investigate the interdependencies between variables. As a result, 216 senior and middle managers/owners of SMEs in Ghana were surveyed using a purposive and convenient sampling method. SPSS version 23 and Smart PLS version 3 were used to conduct the research.FindingsWhile the direct link among BI, SCA and OP was confirmed. The outcome also showed that SCA plays a significant mediating role between BI and OP among SMEs.Practical implicationsThe outcome of the study indicates that SCA encourages the use of BI to generate superior OP among SMEs. This knowledge will improve the performance of SMEs and their ability to withstand the competition in the global market.Originality/valueWith the discovery of this study, the theory of a resource-based view now has some empirical evidence behind it. As a result, SMEs prioritize aspects that could improve their operations and implement tactics that would nurture better performance and competitive advantages.
Time and frequency uncertainty spillover among macro uncertainty, financial stress and asset markets
Authors: Sawarn Ujjawal , Dash Pradyumna 
Published: January 2023

Purpose
This study aims to examine the uncertainty spillover among eight important asset classes (cryptocurrencies, US stocks, US bonds, US dollar, agriculture, metal, oil and gold) using weekly data from 2014 to 2020. This study also examines the US macro uncertainty and US financial stress spillover on these assets.

Design/methodology/approach
The authors use time–frequency connectedness method to study the uncertainty spillover among the asset classes.

Findings
This study’s findings revealed that the uncertainty spillover is time-varying and peaked during the 2016 oil supply glut and COVID-19 pandemic. US stocks are the highest transmitter of uncertainty to all other assets, followed by the US dollar and oil. US stocks (US dollar and oil) transmit uncertainty in long (short) term. Furthermore, US macro uncertainty is the net transmitter of uncertainty to the US stocks, industrial metals and oil markets. In contrast, US financial stress is the net transmitter of uncertainty to the US bonds, cryptocurrencies, the US dollar and gold markets. US financial stress (US macro uncertainty) has long (short)-term effects on asset price volatility.

Originality/value
This study complements the studies on volatility spillover among the important asset classes. This study also includes recently financialized asset classes such as cryptocurrencies, agricultural and industrial commodities. This study examines the macro uncertainty and financial stress spillover on these assets.
Linking rhizosphere soil microbial activity and plant resource acquisition strategy
Authors: Han Mengguang , Chen Ying , Sun Lijuan , Yu Miao , Li Rui , Li Shuaifeng , Su Jianrong , Zhu Biao 
Published: January 2023

Abstract

Plants live in association with a diversity of soil microorganisms, which are extremely important in affecting plant growth and soil biogeochemical cycling.

By adopting plant trait‐based approaches, we explored the linkages between rhizosphere soil microbial activity and plant resource acquisition strategy of above‐ and below‐ground across a range of tree species in a subtropical evergreen mixed forest. The microbial activities were represented by diverse extracellular enzymes relevant to carbon, nitrogen and phosphorus cycling and soil organic carbon (SOC) mineralization.

At the species level, leaf and root traits were mainly represented by two leading dimensions, that is, the ‘fast‐slow’ economics spectrum on which leaf and root traits were well aligned and the orthogonal collaboration gradient in the root.

Both extracellular enzymes and SOC mineralization in the rhizosphere varied greatly across plant species. We found that diverse rhizosphere soil microbial activities positively correlated with the classical ‘fast‐slow’ conservation gradient of plant resource acquisition (especially above‐ground), that is, the rhizosphere soil microbes associated with fast‐growing plant species feature higher metabolism than that of slow‐growing plant species. In comparison, rhizosphere soil microbial activities were independent of the plant collaboration gradient in the root, and it might be an alternative exploitative strategy in foraging soil nutrients for plants.

Synthesis. Our study strengthens the multivariate nature of plant resource acquisition in adapting to above‐ and below‐ground stresses. The findings on the linkages between rhizosphere soil microbial activity and plant resource acquisition strategy have the potential to improve our understanding and prediction of plant species turnover impacts on soil biogeochemical cycles.
RISK AND RETURNS: Large‐scale Funding for Urban Research
Authors: Keith Michael , Parnell Susan 
Published: January 2023

AbstractThe nature of research funding shapes knowledge outcomes, especially for urban research that is conducted in multiple sites and over multiple years. Recent unplanned cuts in the Global Challenges Research Fund (GCRF) grants, alongside the rupture caused by Covid‐19, created ethical and procedural issues for completing the PEAK Urban programme. Building on durable partnerships, setting principles for the reduced fund distribution and adjusting modes of working enabled PEAK Urban to navigate the fiscal disruption—but the difficult episode highlights lessons for the ethical organization of global urban research under conditions of uncertainty.
THE PARADOX: Economic Growth that Endangers the Future of Research in Colombia
Authors: Duque Juan C. 
Published: January 2023

AbstractIn this paper I analyse how the gradual reduction of research funds in Colombia can interrupt a key process in the generation of solutions to global urban problems. I draw on a bibliometric analysis to show that research funding flowing from North to South has created collaborations between researchers from North and South that have led to a better and more comprehensive understanding of the challenges facing cities around the world. To conclude, I propose four options to counteract these trends towards lower research funding.
THE BENEFITS OF LARGE‐SCALE, MULTI‐SITE INTERNATIONALLY FUNDED RESEARCH PROJECTS: An Example of New Academic Insights and New Funding Possibilities
Authors: Tucker Andrew 
Published: January 2023

AbstractThere are a number of benefits to large‐scale, multi‐site, internationally funded urban research projects if they are operationalized in ways that acknowledge forms of knowledge incommensurability and allow for space to explore the commensurability of different forms of knowledge across different geographies. Such projects are especially important in the context of the precarity of research funding, as such projects can provide the space to explore new urban research avenues that may not have formed core components of the existing urban studies canon and may also bring with them significant and new funding possibilities. In this intervention I use the example of the GCRF‐funded PEAK Urban programme to consider how new urban research questions related to sexuality and health were brought to the fore during the life of the programme, which offer not only new research avenues, but also potential access to significant new funding sources. Programmes such as PEAK Urban therefore have the potential to build the long‐term resilience of urban research. Cutting funding to such programmes may therefore limit the efficacy of the programmes themselves and the long‐term sustainability of urban scholarship.
BarKeeper—a versatile web framework to assemble, analyse and manage DNA barcoding data and metadata
Authors: Wiechers Sarah , Kösters Lara Marie , Quandt Dietmar , Borsch Thomas , Wicke Susann , Müller Kai Frank 
Published: January 2023

Abstract

DNA barcoding aids in rapidly identifying specimens in various contexts by comparing short stretches of DNA to a reference database. Barcoding initiatives generate large reference databases of carefully curated high‐quality sequences to maximize identification success.

BarKeeper provides a flexible tool for barcoding initiatives and in the context of phylogenetic studies to foster shared work on large datasets of raw sequence and associated metadata. It is free, open‐source and available as a set of Docker containers for easy setup. After setting it up once, all project members can use it independently of their operating system or location.

BarKeeper offers features to collect and manage data and metadata about specimens, taxa and DNA sequences from Sanger sequencing and high‐throughput sequencing (HTS) technologies. It provides excellent flexibility by not being restricted to specific markers or taxon groups. Users can view and edit records and associated metadata while the app assists them by trimming and assembling reads. It automatically checks the quality of generated barcodes with a taxonomy‐based tool and offers a wide range of options for data analysis. Extensive search features allow querying the database for specific groups of records and saving the search results in the user's profile or downloading them in various file formats.

BarKeeper combines multiple tools to aid barcoding projects in every step, from a reference taxa list to finished barcode sequences, thereby minimizing the number of laborious, potentially error‐prone manual steps and enabling efficient collaborative workflows.
Dissemination and implementation of age‐friendly care and geriatric emergency department accreditation at Veterans Affairs hospitals
Authors: Hwang Ula , Runels Tessa , Han Ling , Gruber Erica , McQuown Colleen M. , Ragsdale Luna , Jetter Ethan , Rossomano Nicole , Javier Denise 
Published: January 2023

AbstractObjectivesIn 2018, the U.S. Department of Veterans Affairs (VA) National Office of Geriatrics and Extended Care (GEC) and the National Emergency Medicine (EM) Program partnered to improve emergency care for older Veterans. A core team disseminated age‐friendly models of care via education and standardization of practice with the goal of multisite geriatric emergency department (GED) accreditation. We compare rates of GED screening at VAs with GED implementation to those without.MethodsObservational evaluation of GED screening of older Veterans (≥65 years) at VA Emergency Departments (ED) from January 2018 to March 2022, during peak pandemic years. Data were extracted from the VA Corporate Data Warehouse of Veteran ED visit encounters to track documented GED screens and Veteran demographic data. Generalized estimating equation models were used to compare screening completion across different levels of GED accreditation, adjusting for potential confounding.ResultsDuring this period, over 1.07 million Veterans ≥ 65 years of age made 4.07 million VA ED visits. Mean (±SD) age was 73.4 (±7.2) years, 96.5% were male, 68% were White, and 89.9% made their index ED visit at a non‐GED VA ED. As of early 2022, a total of 50 of 111 VA EDs have achieved or applied for GED accreditation. During early 2022, 8.3% of all visits by older Veterans had at least one GED screen documented; 15% were screened at Levels 1–3 GED versus 2.2% at non‐GED facilities. Screens identifying older adults at risk for poor outcomes, for delirium, and for falls had the highest usage rates within VA GEDs. Veterans seen at Level 1 GEDs had a 76‐fold greater odds of having a GED screen than at Level 3 GEDs (odds ratio 75.8, 95% confidence interval 72.8–79.0).ConclusionsThrough VA National Office of GEC and EM Program partnership, the VA has created, standardized, and disseminated a GED Model of Care, despite the pandemic. GED accreditation was associated with GED screen implementation, with Level 1 having the highest screening prevalence.