bluuebunny/crossref_metadata_2025_split huggingface dataset:
An unusual case of small bowel obstruction because of body packing
Authors: Boucher Alexandra L , Shah Nikunj , Brynien Daniel H , Bregg Jason M , Yokota Shinichiro 
Published: April 2023

Abstract
Body packing is the internal concealment of illicit drugs for the purpose of transportation and evasion of law enforcement. It is associated with medical complications such as acute toxicity from ingested drug, bowel obstruction or perforation. It is rare to require surgical intervention with a small amount of ingested drug packet. Here, we present the case of a young adult male who presented with abdominal pain for 3 days. The patient admitted ingesting a condom filled with suboxone several years ago and denied recent ingestion. The patient was found to have small bowel obstruction with ingested foreign body being a transition point on CT scan, requiring exploratory laparotomy and small bowel resection.
Structural, nonclinical, and clinical features of ozoralizumab: A novel tumour necrosis factor inhibitor
Authors: Takeuchi Tsutomu 
Published: April 2023

ABSTRACT
Tumour necrosis factor (TNF) inhibitors are currently the most widely used biological agents to treat rheumatoid arthritis. Ozoralizumab (OZR), a novel TNF inhibitor, is an antibody using variable heavy-chain domains of heavy-chain antibody (VHHs) and became the first VHH drug approved for the treatment of rheumatoid arthritis in September 2022. VHHs isolated from camelid heavy-chain antibodies can bind antigens with a single molecule. OZR is a trivalent VHH that consists of two anti-human TNFα VHHs and one anti–human serum albumin (anti-HSA) VHH. This review summarizes OZR’s unique structural characteristics and nonclinical and clinical data. The clinical data outline the pharmacokinetics, efficacy, relationship between efficacy and pharmacokinetics, and safety of OZR, focusing on a Phase II/III confirmatory study (OHZORA trial).
Quantitative elemental imaging in eukaryotic algae
Authors: Schmollinger Stefan , Chen Si , Merchant Sabeeha S 
Published: April 2023

AbstractAll organisms, fundamentally, are made from the same raw material, namely the elements of the periodic table. Biochemical diversity is achieved by how these elements are utilized, for what purpose, and in which physical location. Determining elemental distributions, especially those of trace elements that facilitate metabolism as cofactors in the active centers of essential enzymes, can determine the state of metabolism, the nutritional status, or the developmental stage of an organism. Photosynthetic eukaryotes, especially algae, are excellent subjects for quantitative analysis of elemental distribution. These microbes utilize unique metabolic pathways that require various trace nutrients at their core to enable their operation. Photosynthetic microbes also have important environmental roles as primary producers in habitats with limited nutrient supplies or toxin contaminations. Accordingly, photosynthetic eukaryotes are of great interest for biotechnological exploitation, carbon sequestration, and bioremediation, with many of the applications involving various trace elements and consequently affecting their quota and intracellular distribution. A number of diverse applications were developed for elemental imaging, allowing subcellular resolution, with X-ray fluorescence microscopy (XFM, XRF) being at the forefront, enabling quantitative descriptions of intact cells in a non-destructive method. This Tutorial Review summarizes the workflow of a quantitative, single-cell elemental distribution analysis of a eukaryotic alga using XFM.
Take Care
Authors: Lett Stephen 
Published: April 2023

AbstractIn this rejoinder, I elaborate on the uncomfortable and foundational call of “Making a Home of the Society for Music Theory, Inc.”: the necessity of abolition as at once the destruction of our colonial/capitalist worlding and the building of a life-affirming world otherwise. Bringing to the fore a couple of backgrounds to my writing—early career transience and building community with unhoused residents of Norman, Oklahoma—I clarify that my call is not for inclusion (and enclosure) within this colonial/capitalist worlding, but our collective liberation from it. Ultimately, I hope this response might inspire readers to build life-affirming, abolitionist worlds wherever they find themselves.
e-RNA: a collection of web-servers for the prediction and visualisation of RNA secondary structure and their functional features
Authors: Tsybulskyi Volodymyr , Semenchenko Egor , Meyer Irmtraud M 
Published: April 2023

Abstract
e-RNA is a collection of web-servers for the prediction and visualisation of RNA secondary structures and their functional features, including in particular RNA–RNA interactions. In this updated version, we have added novel tools for RNA secondary structure prediction and have significantly updated the visualisation functionality. The new method CoBold can identify transient RNA structure features and their potential functional effects on a known RNA structure during co-transcriptional structure formation. New tool ShapeSorter can predict evolutionarily conserved RNA secondary structure features while simultaneously taking experimental SHAPE probing evidence into account. The web-server R-Chie which visualises RNA secondary structure information in terms of arc diagrams, can now be used to also visualise and intuitively compare RNA–RNA, RNA–DNA and DNA–DNA interactions alongside multiple sequence alignments and quantitative information. The prediction generated by any method in e-RNA can be readily visualised on the web-server. For completed tasks, users can download their results and readily visualise them later on with R-Chie without having to re-run the predictions. e-RNA can be found at http://www.e-rna.org.
A Phase II Open-Label Trial of Binimetinib and Hydroxychloroquine in Patients With Advanced KRAS-Mutant Non-Small Cell Lung Cancer
Authors: Aggarwal Charu , Maity Alisha P , Bauml Joshua M , Long Qi , Aleman Tomas , Ciunci Christine , D’Avella Christopher , Volpe Melissa , Anderson Evan , Jones Lisa McCormick , Sun Lova , Singh Aditi P , Marmarelis Melina E , Cohen Roger B , Langer Corey J , Amaravadi Ravi 
Published: April 2023

Abstract

Background
In RAS-mutant tumors, combined MEK and autophagy inhibition using chloroquine demonstrated synthetic lethality in preclinical studies. This phase II trial evaluated the safety and activity of the MEK inhibitor binimetinib combined with hydroxychloroquine (HCQ) in patients with advanced KRAS-mutant non-small cell lung cancer (NSCLC).

Methods
Eligibility criteria included KRAS-mutant NSCLC, progression after first-line therapy, ECOG PS 0-1, and adequate end-organ function. Binimetinib 45 mg was administered orally (p.o.) bid with HCQ 400 mg p.o. bid. The primary endpoint was objective response rate (ORR). A Simon’s 2-stage phase II clinical trial design was used, with an α error of 5% and a power β of 80%, anticipating an ORR of 30% to proceed to the 2-stage expansion.

Results
Between April 2021 and January 2022, 9 patients were enrolled to stage I: median age 64 years, 44.4% females, 78% smokers. The best response was stable disease in one patient (11.1%). The median progression free survival (PFS) was 1.9 months, and median overall survival (OS) was 5.3 months. Overall, 5 patients (55.6%) developed a grade 3 adverse event (AE). The most common grade 3 toxicity was rash (33%). Pre-specified criteria for stopping the trial early due to lack of efficacy were met.

Conclusion
The combination of B + HCQ in second- or later-line treatment of patients with advanced KRAS-mutant NSCLC did not show significant antitumor activity. (ClinicalTrials.gov Identifier: NCT04735068).
An unexpected hydratase synthesizes the green light-absorbing pigment fucoxanthin
Authors: Cao Tianjun , Bai Yu , Buschbeck Paul , Tan Qiaozhu , Cantrell Michael B , Chen Yinjuan , Jiang Yanyou , Liu Run-Zhou , Ries Nana K , Shi Xiaohuo , Sun Yan , Ware Maxwell A , Yang Fenghua , Zhang Huan , Han Jichang , Zhang Lihan , Huang Jing , Lohr Martin , Peers Graham , Li Xiaobo 
Published: April 2023

AbstractThe ketocarotenoid fucoxanthin and its derivatives can absorb blue–green light enriched in marine environments. Fucoxanthin is widely adopted by phytoplankton species as a main light-harvesting pigment, in contrast to land plants that primarily employ chlorophylls. Despite its supreme abundance in the oceans, the last steps of fucoxanthin biosynthesis have remained elusive. Here, we identified the carotenoid isomerase-like protein CRTISO5 as the diatom fucoxanthin synthase that is related to the carotenoid cis–trans isomerase CRTISO from land plants but harbors unexpected enzymatic activity. A crtiso5 knockout mutant in the model diatom Phaeodactylum tricornutum completely lacked fucoxanthin and accumulated the acetylenic carotenoid phaneroxanthin. Recombinant CRTISO5 converted phaneroxanthin into fucoxanthin in vitro by hydrating its carbon–carbon triple bond, instead of functioning as an isomerase. Molecular docking and mutational analyses revealed residues essential for this activity. Furthermore, a photophysiological characterization of the crtiso5 mutant revealed a major structural and functional role of fucoxanthin in photosynthetic pigment–protein complexes of diatoms. As CRTISO5 hydrates an internal alkyne physiologically, the enzyme has unique potential for biocatalytic applications. The discovery of CRTISO5 illustrates how neofunctionalization leads to major diversification events in evolution of photosynthetic mechanisms and the prominent brown coloration of most marine photosynthetic eukaryotes.
Julius Caesar and the Revenge Plot from Oxford to Shakespeare’s Globe
Authors: Vozar Thomas Matthew 
Published: April 2023

Abstract
Several attempts have been made to demonstrate that Shakespeare knew and drew from the anonymous university drama Caesars Reuenge in writing Julius Caesar. This article revisits the question of influence, making the case that Reuenge not only influenced certain particulars of Shakespeare’s play but also helped to shape its general structure and suggested some of its major themes. Following the introduction, the first part situates Reuenge, performed by the students of Trinity College, Oxford, within its academic context, and examines the crucial matter of dating, arguing that it precedes Shakespeare’s Caesar tragedy. The second part treats the minor and major parallels between the two plays and demonstrates the ways in which Shakespeare’s academic predecessor seems to haunt the familiar plot of Julius Caesar. A brief conclusion considers what this might indicate about Shakespeare’s relationship with the universities, a subject of increasing scholarly attention.
“The Tables Are Turning”: The Evangelical Defense of Anti-LGBTQ+ Religious Liberty
Authors: McDowell Amy , Ward Pace T 
Published: April 2023

Abstract
This article extends research on how dominant groups use the rhetoric of marginality to defend and reinforce their control of the public sphere. We conducted interviews with evangelical churchgoers in Mississippi to understand the evangelical support for religious accommodations that privilege conservative Christian beliefs about sex, gender, and marriage. We found that rather than cite scripture about homosexuality or Godly marriage, churchgoers instead told stories about Christian maltreatment and censorship to defend the idea that LGBTQ+ individuals should stay in the closet or find alternatives to the people and places that will refuse service to them. Our findings shed critical light on how evangelical churchgoers accommodate Christian nationalism—or the ideological movement to put Christians back in charge of America—in a context where conservative Christians already enjoy unmatched social and political advantages.
Moral Disengagement in Social Work
Authors: Reamer Frederic G 
Published: April 2023

Abstract
In recent years, social workers have paid increased attention to ethical issues. The profession’s literature has burgeoned on topics such as ethical dilemmas in social work practice, ethical decision making, boundary issues and dual relationships, ethics-related risk management, and moral injury. This noteworthy trend builds on social work’s rich and long-standing commitment to the development of core values and ethical standards evident throughout its history. Unlike allied human service and behavioral health professions, social work’s ethics-related literature has not focused on the critically important issue of moral disengagement. Moral disengagement is typically defined as the process whereby individuals convince themselves that ethical standards do not apply to them. In social work, moral disengagement can lead to ethics violations and practitioner liability, particularly when social workers believe that they are not beholden to widely embraced ethical standards in the profession. The purpose of this article is to explore the nature of moral disengagement in social work, identify possible causes and consequences, and present meaningful strategies designed to prevent and respond to moral disengagement in the profession.
Similar patterns of leaf temperatures and thermal acclimation to warming in temperate and tropical tree canopies
Authors: Crous K Y , Cheesman A W , Middleby K , Rogers E I E , Wujeska-Klause A , Bouet A Y M , Ellsworth D S , Liddell M J , Cernusak L A , Barton C V M 
Published: April 2023

Abstract
As the global climate warms, a key question is how increased leaf temperatures will affect tree physiology and the coupling between leaf and air temperatures in forests. To explore the impact of increasing temperatures on plant performance in open air, we warmed leaves in the canopy of two mature evergreen forests, a temperate Eucalyptus woodland and a tropical rainforest. The leaf heaters consistently maintained leaves at a target of 4 °C above ambient leaf temperatures. Ambient leaf temperatures (Tleaf) were mostly coupled to air temperatures (Tair), but at times, leaves could be 8–10 °C warmer than ambient air temperatures, especially in full sun. At both sites, Tleaf was warmer at higher air temperatures (Tair &gt; 25 °C), but was cooler at lower Tair, contrary to the ‘leaf homeothermy hypothesis’. Warmed leaves showed significantly lower stomatal conductance (−0.05 mol m−2 s−1 or −43% across species) and net photosynthesis (−3.91 μmol m−2 s−1 or −39%), with similar rates in leaf respiration rates at a common temperature (no acclimation). Increased canopy leaf temperatures due to future warming could reduce carbon assimilation via reduced photosynthesis in these forests, potentially weakening the land carbon sink in tropical and temperate forests.
Effects of different phosphorus levels on tiller bud development in hydroponic Phyllostachys edulis seedlings
Authors: Lin Chenjun , Hang Tingting , Jiang Chenhao , Yang Ping , Zhou Mingbing 
Published: April 2023

Abstract
An appropriate amount of phosphate fertilizer can improve the germination rate of bamboo buds and increase the bamboo shoot output. However, the underlying biological mechanisms of phosphate fertilizer in bamboo shoot development have not been systematically reported. Herein, the effects of low (LP, 1 μM), normal (NP, 50 μM) and high (HP, 1000 μM) phosphorus (P) on the growth and development of moso bamboo (Phyllostachys edulis) tiller buds were first investigated. Phenotypically, the seedling biomass, average number of tiller buds and bud height growth rate under the LP and HP treatments were significantly lower than those under the NP treatment. Next, the microstructure difference of tiller buds in the late development stage (S4) at three P levels was analyzed. The number of internode cells and vascular bundles were significantly lower in the LP treatments than in the NP treatments. The relative expression levels of eight P transport genes, eight hormone-related genes and four bud development genes at the tiller bud developmental stage (S2–S4) and the tiller bud re-tillering stage were analyzed with real-time polymerase chain reaction. The results showed that the expression trends for most P transport genes, hormone-related genes and bud development genes from S2 to S4 were diversified at different P levels, and the expression levels were also different at different P levels. In the tiller bud re-tillering stage, the expression levels of seven P transport genes and six hormone-related genes showed a downward trend with increasing P level. REV expression level decreased under LP and HP conditions. TB1 expression level increased under HP condition. Therefore, we conclude that P deficiency inhibits tiller bud development and re-tillering, and that P depends on the expression of REV and TB1 genes and auxin, cytokinin and strigolactones synthesis and transporter genes to mediate tiller bud development and re-tillering.
Biologicals in IgE-mediated food allergy
Authors: Mutarelli Antonio , Giavina-Bianchi Bruna , Arasi Stefania , Cafarotti Arianna , Fiocchi Alessandro 
Published: April 2023

Purpose of review
A better understanding of the most recent scientific literature in the use of biological therapy in the treatment of patients with IgE-mediated food allergy.

Recent findings
A systematic review and meta-analysis demonstrated safety and effectiveness of omalizumab in the treatment of food allergy. The findings support the potential use of omalizumab as a monotherapy or as an adjunct to oral immunotherapy in IgE-mediated cow's milk allergy. The potential use of other biologics in the management of food allergy is subject of speculation.

Summary
Different biological therapies are under evaluation for food allergic patients. The advance in literature will guide for a personalized treatment in the near future. However, additional research is needed to better understand the best candidate for each treatment, the optimal dose and timing.
Food-labeling issues for severe food-allergic consumers
Authors: Dinardo Giulio , Fierro Vincenzo , del Giudice Michele Miraglia , Urbani Sara , Fiocchi Alessandro 
Published: April 2023

Purpose of review
Food label represent the primary and only source of information before consuming a food product. Deputy government agencies on five continents require allergenic ingredients to be declared when used in prepackaged foods to assist patients in identifying allergenic foods and to help them to choose wisely. Unfortunately, the mandatory allergen list and legislation regarding food labels and reference doses is not uniform and varies by country. This may add difficulties to food-allergic patients, especially those with severe food allergy.

Recent findings
A new definition of food allergy severity (the DEFASE grid, developed by the World Allergy Organization), aims to assist clinicians in the identification of the at-risk patients. The FASTER ACT and Natasha's Laws has made important improvements such as the entry of sesame as a major allergen in the United States, and increased evidence of allergens on the food label within prepackaged for direct sale (PPDS) in the UK. The recent introduction of Vital 3.0 brought important new features such as an updated reference doses for many foods.

Summary
Currently, there are still considerable differences regarding food labels in different countries. Growing public and scientific attention to the problem promises to increase the food safety for allergens. Among the next improvements, we expect a reconsideration of the food reference doses, a harmonization of the food oral challenge process, and the promulgation of regulatory rules for the precautionary labeling.
The development of the DEFASE project
Authors: Arasi Stefania , Fiocchi Alessandro 
Published: April 2023

Purpose of review
To familiarize the reader with the milestones of the DEFASE (Definition of Food Allergy Severity) project. This World Allergy Organization (WAO) initiative has recently developed the first international consensus-based classification system of severity associated with IgE-mediated food allergy as a whole disease incorporating multidisciplinary perspectives from different stakeholders involved.

Recent findings
After a systematic review of current evidence on the definition of severity of food allergy, an e-Delphi methodology has been applied to obtain the consensus/agreement through repeated rounds of survey. This comprehensive scoring system has been developed to be used in research settings in its current version with the scope of stratifying the severity of a clinical situation of food allergy.

Summary
Notwithstanding the limitations due to the complexity of the matter, the recent developed DEFASE definition will be relevant for establishing the levels of diagnostic, management and therapeutic commitment for the disease in the various geographical contexts. Future research should focus on internal and external validation of the scoring system, tailoring of these models to different food allergenic sources, populations, and settings.
Clinical outcomes of efficacy in food allergen immunotherapy trials
Authors: Bartha Irene , Rodríguez Del Río Pablo 
Published: April 2023

Purpose of review
With food allergy affecting millions of children worldwide, the consolidation of food allergen immunotherapy represents an encouraging therapeutic option, that might expand in the next few years to reach greater number of candidates. This review aims at providing a critical overview of the efficacy outcomes employed in food allergen immunotherapy trials (AIT).

Recent findings
Understanding efficacy endpoints rely on identifying what and how these are being measured. Desensitization, as the efficacy of the therapy to increase the patient's reactivity threshold to the food during therapy, and Sustained Unresponsiveness, withholding such efficacy even if the therapy is withdrawn, are nowadays considered the main parameters of efficacy evaluation. Quality of life is a promising variable to capture food AIT impact from the patient's perspective.
There is a relevant degree of heterogeneity across studies in outcomes definitions and also in oral food challenges design, the tool that is more spread to assess results, hampering study comparison.

Summary
Interpreting the results of a clinical trial, and comparing data from different studies is an important task, both for the researcher and the clinician, that should be done after a careful analysis of the outcomes and the evaluation tools used.
Relationship between eliciting doses and the severity of allergic reactions to food
Authors: Yanagida Noriyuki , Sato Sakura , Ebisawa Motohiro 
Published: April 2023

Purpose of review
Food allergies with low thresholds are associated with frequent accidental allergic reactions. Severe reactions attributable to accidental ingestion often result in a poor quality of life. Nevertheless, no evidence of a relationship between a low threshold dose and the severity of symptoms exists. Therefore, we evaluated recent data regarding the threshold of food allergies based on the oral food challenge (OFC). We also proposed a stepwise OFC method to identify threshold and consumable doses.

Recent findings
A higher specific IgE level and a history of food-induced anaphylaxis were related to low threshold doses and severe reactions during the OFC. In addition, a low threshold dose was not directly correlated with severe reactions. Performing a stepwise OFC may help safely clarify consumable doses and prevent the complete avoidance of allergy-causing foods.

Summary
Severe food allergies with high specific IgE levels are associated with lower thresholds and more severe reactions. However, the threshold is not directly related to the severity of food-induced allergic symptoms. Identifying a well tolerated consumable dose using a stepwise OFC may help manage food allergies.