bluuebunny/crossref_metadata_2025_split huggingface dataset:
TaTPP‐7A positively feedback regulates grain filling and wheat grain yield through T6P‐SnRK1 signalling pathway and sugar–ABA interaction
Authors: Liu Hongxia , Si Xuemei , Wang Zhenyu , Cao Liangjing , Gao Lifeng , Zhou Xiaolong , Wang Wenxi , Wang Ke , Jiao Chengzhi , Zhuang Lei , Liu Yunchuan , Hou Jian , Li Tian , Hao Chenyang , Guo Weilong , Liu Jun , Zhang Xueyong 
Published: February 2023

SummaryGrain size and filling are two key determinants of grain thousand‐kernel weight (TKW) and crop yield, therefore they have undergone strong selection since cereal was domesticated. Genetic dissection of the two traits will improve yield potential in crops. A quantitative trait locus significantly associated with wheat grain TKW was detected on chromosome 7AS flanked by a simple sequence repeat marker of Wmc17 in Chinese wheat 262 mini‐core collection by genome‐wide association study. Combined with the bulked segregant RNA‐sequencing (BSR‐seq) analysis of an F2 genetic segregation population with extremely different TKW traits, a candidate trehalose‐6‐phosphate phosphatase gene located at 135.0 Mb (CS V1.0), designated as TaTPP‐7A, was identified. This gene was specifically expressed in developing grains and strongly influenced grain filling and size. Overexpression (OE) of TaTPP‐7A in wheat enhanced grain TKW and wheat yield greatly. Detailed analysis revealed that OE of TaTPP‐7A significantly increased the expression levels of starch synthesis‐ and senescence‐related genes involved in abscisic acid (ABA) and ethylene pathways. Moreover, most of the sucrose metabolism and starch regulation‐related genes were potentially regulated by SnRK1. In addition, TaTPP‐7A is a crucial domestication‐ and breeding‐targeted gene and it feedback regulates sucrose lysis, flux, and utilization in the grain endosperm mainly through the T6P‐SnRK1 pathway and sugar–ABA interaction. Thus, we confirmed the T6P signalling pathway as the central regulatory system for sucrose allocation and source–sink interactions in wheat grains and propose that the trehalose pathway components have great potential to increase yields in cereal crops.
Minimal invasiveness in lateral bone augmentation with simultaneous implant placement: A systematic review
Authors: Severi Mattia , Trombelli Leonardo , Heitz‐Mayfield Lisa , Farina Roberto , Simonelli Anna 
Published: February 2023

AbstractThe presence of a peri‐implant bone dehiscence (BD) or fenestration (BF) is a common finding after implant placement in a crest with a reduced bucco‐lingual bone dimension. The presence of a residual BD is associated with a relevant incidence of peri‐implant biological complications over time. Guided bone regeneration (GBR), performed at implant placement, is the most validated treatment to correct a BD. In the present systematic review, the evidence evaluating factors which could reduce the invasiveness of a GBR procedure with respect to patient‐reported outcomes, intra‐ and post‐ surgical complications, was summarized. Factors included were: technical aspects, regenerative materials for GBR, and peri‐ and post‐operative pharmacological regimens. The available evidence seems to indicate that the use of membrane fixation and flap passivation by means of a double flap incision technique may reduce the incidence of post‐surgical complications. When feasible, the coronal advancement of the lingual flap is suggested. The use of a non‐cross linked resorbable membrane positively impacts on patient discomfort. The adjunctive use of autogenous bone to a xenograft seems not to improve BD correction, but could increase patient discomfort. Systemic antibiotic administration after a GBR procedure does not seem to be justified in systemically healthy patients.
Development is in the details: Event‐related theta oscillations reveal children and adults verify multiplication facts differently
Authors: Wood Matthew , Grenier Amandine E. , Wicha Nicole Y. Y. 
Published: February 2023

AbstractWhen verifying the correctness of single‐digit multiplication problems, children and adults show a robust ERP correctness effect thought to reflect similar cognitive processes across groups. Recent studies suggest that this effect is instead a modulation of the negative‐going N400 component in children, reflecting access to semantic memory, and the positive‐going P300 component in adults, reflecting stimulus categorization. However, the relative difference in ERP amplitude is the same for both components, more positive for correct than incorrect solutions, presenting a challenge to ascertaining the appropriate interpretation. Time‐frequency analysis (TFA) of the N400/P300 window provides an objective approach to dissociating these effects. TFA measured from solution onset during single‐digit multiplication verification revealed significant modulations of event‐related as theta power (3–6 Hz) in both groups. Correct trials elicit less power in children (9–12 years) and more power in adults relative to incorrect trials. These findings are consistent with modulations of the N400 and P300, respectively, where opposite effects were predicted for spectral power. The ERP results further support a reinterpretation of the multiplication correctness effect. In contrast, TFA of the N400 effect elicited to a word‐picture verification task revealed the same event‐related theta effect in both groups, with increased power for mismatched than matched pictures. Together, these findings provide evidence for a developmental shift in cognitive processing specific to the multiplication task. Models of arithmetic should account for this overlooked difference in cognitive processing between children and adults.
Powerful stories of local climate action: Comparing the evolution of narratives using the “narrative rate” index
Authors: Nagel Melanie , Schäfer Melanie 
Published: February 2023

AbstractThe complex relationship between local climate action and reducing global emissions is often difficult to grasp and cities might scale back their ambitions if they realize that the way is hard and rocky. We analyze local climate change narratives through the lens of the narrative policy framework (NPF). Following the NPF, policy actors use narratives to communicate information to change an existing system in order to achieve a specific goal. For our study, this implies that local climate action can be improved by using narratives to communicate information to achieve climate neutrality. We focus on the position and interplay of the most important and dominant actors and concepts in the plot of the narratives. To better analyze the main actors and content of the NPF plot in a systematic and comparable way, we offer the “narrative rate index” to dynamically trace the narratives. To include the dynamics and effects of global significant developments in the plots of the NPF narratives, we selected three phases between January 2018 and June 2021. What are the main characters and dominant actors in the plot of the narratives in the two cases? Are the narratives different depending on the context of the city, and are there general observable patterns? Our findings identify the central actors in the narratives of both cities: the science institute in Karlsruhe and the City Administration in Mannheim. Karlsruhe, a climate change pioneer, has slowed down its climate change activities somewhat, while Mannheim is a latecomer with high ambitions.
Anatomy of a diamondiferous gravel barrier spit at the palaeo‐Orange River mouth, south‐western Namibia
Authors: Spaggiari Renato (Spaggs) , Bordy Emese M. 
Published: February 2023

ABSTRACTThe sedimentary facies architecture of a Plio‐Pleistocene diamond‐bearing gravel barrier spit within the palaeo‐Orange River mouth in south‐western Namibia has been revealed during onshore mining activities. Four sedimentary facies associations build this ancient coastal landform and have been interpreted, from a seaward to landward direction, as sediments in: (i) the foreshore, represented by an intertidal beach; (ii) a subtidal spit platform on which the spit developed; (iii) storm‐constructed spit head recurves on the landward side of the spit; and (iv) an estuarine back‐barrier. This sedimentary record reflects an evolving spit that grew in response to a combination of marine and fluvial processes in a wave‐dominated, high‐energy coastal regime. Here, the southern Atlantic Ocean aggressively dispersed sediment delivered by a Plio‐Pleistocene Orange River, ensuring that the fine‐grained sediments were removed to allow only the gravel fraction to build up along the coastline. This extreme dispersal of sediment also precluded the construction of a classic delta; hence sediment storage offshore of the river mouth was limited and spit growth was dependent on the direct supply of river sediment. In response to the intermittent supply of sediment, spit growth and decay alternated during periods of sediment abundance and starvation, respectively. During positive sediment budget, the spit extended northward and cut off parts of the mainland to form an estuarine back‐barrier. Foreshore and spit head progradation was sustained under these conditions, and formed a beach‐ridge plain with a topographic expression of several coast‐parallel ridges that curved at the spit head into the back‐barrier. Spit extension and recurve development were controlled by a combination of high sediment supply, a vigorous longshore drift and powerful waves approaching from various directions. This Plio‐Pleistocene coastal landform in Namibia offers a depositional model relevant to gravel‐dominated barrier spits near river mouths along wave‐dominated clastic coasts.
In vitro and in vivo validation of a new three‐dimensional fringe projection‐based device (AEVA‐HE) dedicated to skin surface mapping
Authors: Shaiek Ayet , Monot Muriel , Rubert Virginie , Cornillon Celine , Vicic Marco , Flament Frederic , Decocq Geraldine , Servant Jean‐Jacques , Koeller Gabriel , Lille Christelle 
Published: February 2023

AbstractBackgroundAging signs are much visible on the surface of the skin that presents different changes: cheeks start to sag, more and deeper wrinkles appear, and pigmentation spots increase. Face diagnostic to recommend products includes assessing cutaneous micro‐relief or the micro‐depressive network on the face. Furthermore, there is an increasing demand for clinical and instrumental methods to prove the efficacy of anti‐aging treatments. As a result, very accurate and sensitive three‐dimensional (3D) devices are developed and validated to measure and quantify aging skin and to catch fine anti‐aging products acting on wrinkles and fine lines.MethodsAEVA‐HE, a non‐invasive 3D method based on fringe projection technology, is used to robustly characterize the skin micro‐relief from a full‐face acquisition and from multiple extracted zones of interest. In vitro and in vivo studies are conducted to assess the reproducibility of this system and its precision toward a standard fringe projection system, DermaTOP.ResultsThe AEVA‐HE successfully measured micro‐relief and wrinkles and demonstrated the reproducibility of measurements. AEVA‐HE parameters were found highly correlated to DermaTOP.ConclusionThe present work illustrates the performance of the AEVA‐HE device and its dedicated software kit as a precious tool for quantifying the major characteristics of wrinkles appearing with age and thus offers a high potential for assessing the effect of anti‐wrinkling products.
DNA methylation remodeling in F1 hybrids
Authors: Kakoulidou Ioanna , Johannes Frank 
Published: February 2023

SUMMARYF1 hybrids derived from a cross between two inbred parental lines often display widespread changes in DNA methylation patterns relative to their parents. To which extent these changes drive non‐additive gene expression levels and phenotypic heterosis in F1 individuals is not fully resolved. Current mechanistic models propose that DNA methylation remodeling in hybrids is the result of epigenetic interactions between parental alleles via small interfering RNA (sRNA). These models have strong empirical support but are limited to genomic regions where the two parental lines differ in DNA methylation status. However, most remodeling events occur in parental regions with similar methylation patterns, and seem to be strongly conditioned by distally acting factors, even in isogenic hybrid systems. The molecular basis of these distal interactions is currently unknown, and will likely emerge as an active area of research in the future. Despite these gaps in our molecular understanding, parental DNA methylation states are statistically associated with heterosis, independent of genetic information, and may serve as biomarkers in crop breeding.
Anatomy of the congenital calcaneal bursae in the bovine
Authors: Vermedal Hanna , Zingariello Edoardo , Hoey Seamus E. , De Pedro Anna J. , O'Leary John Mark 
Published: February 2023

AbstractObjectiveThe objective of this study was to determine the anatomical relationship of the congenital calcaneal bursae in the bovine, and describe the computed tomography (CT), endoscopic and gross anatomy of these bursae.Study designEx vivo experimental.Sample populationEighteen clinically normal cadaver bovine hindlimbs.MethodsIntrasynovial injection of iodinated contrast and methylene blue into the intertendinous calcaneal bursa (ICB) (n = 16) or gastrocnemius calcaneal bursa (GCB) (n = 2). Limbs were imaged post‐contrast injection using multidetector CT. Endoscopic examination of the ICB was performed on two randomly selected limbs. All limbs underwent gross anatomical dissection.ResultsThe anatomy of the congenital calcaneal bursae was consistent between CT imaging, endoscopic examination and gross dissection. The ICB and GCB were two separate synovial structures with no communication in all limbs. The distal and proximal extent of the ICB, defined as the distance from the point of tuber calcanei to the distal/proximal aspect of the ICB, was (median [IQR]) 7.4 (7.4 to 7.8) cm distally and 5.4 (4.7 to 6.0) cm proximally.ConclusionPositive contrast CT and gross anatomical dissection revealed no communication between the congenital calcaneal bursae in any limb. Routine bursoscopy allowed complete endoscopic examination of the ICB. The proximal extent of the ICB is shorter than the distal extent. The use of a collective term for these bursae should be avoided in the bovine, as the ICB and the GCB are two separate synovial structures with no communication.Clinical significanceKnowledge of distinct anatomy and relationship between the congenital calcaneal bursae in the bovine may facilitate diagnosis and treatment of disorders affecting the region of tuber calcanei, including septic bursitis and osteomyelitis.
Validation and efficacy of ‘pure’ venous lymph node flap in a rat lymphoedema model
Authors: Cui Chunxiao , Nicoli Fabio , Min Peiru , Li Ke , Zhang Yixin 
Published: February 2023

AbstractThe venous lymph node flap (vLNF) is regarded as an arterialized or ‘pure’ venous flap, subject to which type of vessel provides blood supply to the flap. However, whether the vLNF drainage function is maintainable or sufficient for the treatment of lymphoedema is yet to be elucidated. In this study, the venous flow‐through lymph node flaps or ‘pure’ vLNF was investigated in a rat's tail (lymphoedema model), and the therapeutic effect was analysed to clarify its potential value for the treatment of lymphoedema. The subjects (rats) were divided into three groups, the experimental group (‘pure’ vLNF group), the control group (lymphoedema group), and the baseline group. The tail circumference and diameter were recorded for 5 weeks after the operation. Five weeks after surgery, the lymphatic drainage function was evaluated using indocyanine green (ICG) lymphography, while the skin thickness and collagen layer were assessed by histological analysis. The average diameter and circumference of the experimental group were significantly shorter than the control group (p < 0.01). When comparing ICG lymphography within the three groups, the baseline group showed that the ICG entered the inguinal lymph node and appeared in the liver while the ICG still accumulated in the oedematous tail with no fluorescence emerging in other parts of the rat's body, in the control group. In the experimental group, the ICG entered the vLNF via the flap junction with the rat's tail and fluorescence emerging in the rat's liver. When comparing the three groups above with the control group, the experimental group's skin and collagen layer thickness were significantly improved (p < 0.01). Lymphatic recanalization was shown to appear between ‘pure’ vLNF and the recipient area. Moreover, vLNF was shown to drain lymph fluid via the venous system, thus relieved swelling and fibrosis of the lymphoedematous tissue. Consequently, this could represent a new potential surgical approach for the treatment of lymphoedema.
Evidence‐based practice improves patient outcomes and healthcare system return on investment: Findings from a scoping review
Authors: Connor Linda , Dean Jennifer , McNett Molly , Tydings Donna M. , Shrout Amanda , Gorsuch Penelope F. , Hole Ashley , Moore Laura , Brown Roy , Melnyk Bernadette Mazurek , Gallagher‐Ford Lynn 
Published: February 2023

AbstractBackgroundEvidence‐based practice and decision‐making have been consistently linked to improved quality of care, patient safety, and many positive clinical outcomes in isolated reports throughout the literature. However, a comprehensive summary and review of the extent and type of evidence‐based practices (EBPs) and their associated outcomes across clinical settings are lacking.AimsThe purpose of this scoping review was to provide a thorough summary of published literature on the implementation of EBPs on patient outcomes in healthcare settings.MethodsA comprehensive librarian‐assisted search was done with three databases, and two reviewers independently performed title/abstract and full‐text reviews within a systematic review software system. Extraction was performed by the eight review team members.ResultsOf 8537 articles included in the review, 636 (7.5%) met the inclusion criteria. Most articles (63.3%) were published in the United States, and 90% took place in the acute care setting. There was substantial heterogeneity in project definitions, designs, and outcomes. Various EBPs were implemented, with just over a third including some aspect of infection prevention, and most (91.2%) linked to reimbursement. Only 19% measured return on investment (ROI); 94% showed a positive ROI, and none showed a negative ROI. The two most reported outcomes were length of stay (15%), followed by mortality (12%).Linking Evidence to ActionFindings indicate that EBPs improve patient outcomes and ROI for healthcare systems. Coordinated and consistent use of established nomenclature and methods to evaluate EBP and patient outcomes are needed to effectively increase the growth and impact of EBP across care settings. Leaders, clinicians, publishers, and educators all have a professional responsibility related to improving the current state of EBP. Several key actions are needed to mitigate confusion around EBP and to help clinicians understand the differences between quality improvement, implementation science, EBP, and research.
The post‐arteriole transitional zone: a specialized capillary region that regulates blood flow within the CNS microvasculature
Authors: Mughal Amreen , Nelson Mark T. , Hill‐Eubanks David 
Published: February 2023

AbstractThe brain is an energy hog, consuming available energy supplies at a rate out of all proportion to its relatively small size. This outsized demand, largely reflecting the unique computational activity of the brain, is met by an ensemble of neurovascular coupling mechanisms that link neuronal activity with local increases in blood delivery. This just‐in‐time replenishment strategy, made necessary by the limited energy‐storage capacity of neurons, complicates the nutrient‐delivery task of the cerebral vasculature, layering on a temporo‐spatial requirement that invites ‐ and challenges ‐ mechanistic interpretation. The centre of gravity of research efforts to disentangle these mechanisms has shifted from an initial emphasis on astrocyte‐arteriole‐level processes to mechanisms that operate on the capillary level, a shift that has brought into sharp focus questions regarding the fine control of blood distribution to active neurons. As these investigations have drilled down into finer reaches of the microvasculature, they have revealed an arteriole‐proximate subregion of CNS capillary networks that serves a regulatory function in directing blood flow into and within downstream capillaries. They have also illuminated differences in researchers’ perspectives on the vascular structures and identity of mural cells in this region that impart the vasomodulatory effects that control blood distribution. In this review, we highlight the regulatory role of a variably named region of the microvasculature, referred to here as the post‐arteriole transition zone, in channeling blood flow within CNS capillary networks, and underscore the contribution of dynamically contractile perivascular mural cell – generally, but not universally, recognized as pericytes – to this function.
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Intestinal distension orchestrates neuronal activity in the enteric nervous system of adult mice
Authors: Cavin Jean‐Baptiste , Wongkrasant Preedajit , Glover Joel B. , Balemba Onesmo B. , MacNaughton Wallace K. , Sharkey Keith A. 
Published: February 2023

AbstractThe enteric nervous system (ENS) regulates the motor, secretory and defensive functions of the gastrointestinal tract. Enteric neurons integrate mechanical and chemical inputs from the gut lumen to generate complex motor outputs. How intact enteric neural circuits respond to changes in the gut lumen is not well understood. We recorded intracellular calcium in live‐cell confocal recordings in neurons from intact segments of mouse intestine in order to investigate neuronal response to luminal mechanical and chemical stimuli. Wnt1‐, ChAT‐ and Calb1‐GCaMP6 mice were used to record neurons from the jejunum and colon. We measured neuronal calcium response to KCl (75 mM), veratridine (10 μM), 1,1‐dimethyl‐4‐phenylpiperazinium (DMPP; 100 μM) or luminal nutrients (Ensure®), in the presence or absence of intraluminal distension. In the jejunum and colon, distension generated by the presence of luminal content (chyme and faecal pellets, respectively) renders the underlying enteric circuit unresponsive to depolarizing stimuli. In the distal colon, high levels of distension inhibit neuronal response to KCl, while intermediate levels of distension reorganize Ca2+ response in circumferentially propagating slow waves. Mechanosensitive channel inhibition suppresses distension‐induced Ca2+ elevations, and calcium‐activated potassium channel inhibition restores neuronal response to KCl, but not DMPP in the distended colon. In the jejunum, distension prevents a previously unknown tetrodotoxin‐resistant neuronal response to luminal nutrient stimulation. Our results demonstrate that intestinal distension regulates the excitability of ENS circuits via mechanosensitive channels. Physiological levels of distension locally silence or synchronize neurons, dynamically regulating the excitability of enteric neural circuits based on the content of the intestinal lumen.
imageKey points
How the enteric nervous system of the gastrointestinal tract responds to luminal distension remains to be fully elucidated.
Here it is shown that intestinal distension modifies intracellular calcium levels in the underlying enteric neuronal network, locally and reversibly silencing neurons in the distended regions.
In the distal colon, luminal distension is integrated by specific mechanosensitive channels and coordinates the dynamics of neuronal activation within the enteric network.
In the jejunum, distension suppresses the neuronal calcium responses induced by luminal nutrients.
Physiological levels of distension dynamically regulate the excitability of enteric neuronal circuits.