bluuebunny/crossref_metadata_2025_split huggingface dataset:
Parental and adolescents’ perspectives on environmental predictors of active commuting to school – findings from the ARRIVE mixed-methods study
Authors: Tristram Clara , Marzi Isabel , Beck Franziska , Diehl Katharina , Renninger Denise , Demetriou Yolanda , Krieger Claus , Reimers Anne Kerstin 
Published: January 2025

BackgroundActive commuting to school (e.g., walking or cycling) can contribute to an increase in daily physical activity time of adolescents which is associated with positive health effects. However, it is known that the perceived barriers related to the physical and social environment hinder adolescents in participating in active commuting. To obtain more information about these barriers, and therefore be able to address them in the future, the present study aims to investigate how parents’ and adolescents’ perception of the physical and social environment is associated with (active) commuting to school.MethodsThe ARRIVE study consists of a quantitative online survey with parent-adolescent dyads (N = 517) followed by qualitative interviews with adolescents and parents (N = 32). The quantitative part examined adolescents’ travel behaviour to and from school and its predictors. The qualitative part sought to explore the decision-making process in families regarding transport mode choice. To evaluate predictors of active commuting to and from school binary logistic regression analysis and qualitative content analysis were performed.ResultsIn the quantitative part, we found that adolescents and parents perceive especially having much luggage to carry as crucial for adolescents’ active travel. Besides this barrier on active commuting to and from school found in the questionnaires, parents and adolescents reported lack of social support, weather, convenience, lack of traffic safety and getting a lift as barriers in the interviews.ConclusionWe found differences and similarities in the perceived barriers of active commuting regarding the physical and social environment between adolescents and parents. To encourage adolescents to actively commute to school, the perception of the physical and social environment, especially from parents, be taken into account.
Exploring mosquito virome dynamics within São Paulo Zoo: insights into mosquito-virus-environment interactions
Authors: Guimarães Lilian de Oliveira , Ribeiro Geovani de Oliveira , da Couto Roseane , Ramos Endrya do Socorro Foro , Morais Vanessa dos Santos , Telles-de-Deus Juliana , Helfstein Vanessa Christe , Santos Jesus Maia dos , Deng Xutao , Delwart Eric , Pandey Ramendra Pati , de Camargo-Neves Vera Lucia Fonseca , da Costa Antonio Charlys , Kirchgatter Karin , Leal Élcio 
Published: January 2025

BackgroundMosquito-borne diseases have a significant public health threat worldwide, with arboviruses accounting for a high proportion of infectious diseases and mortality annually. Brazil, in particular, has been suffering outbreaks of diseases transmitted by mosquito viruses, notably those of the Aedes genus, such as dengue, Zika, and chikungunya. Against this background, the São Paulo Zoo is an intriguing ecological niche to explore the virome of mosquitoes, potentially shedding light on the dynamics of arbovirus transmission within a confined setting.MethodsIn this study, we conducted a comprehensive metagenomic analysis of mosquitoes collected from diverse habitats within the zoo, focusing on the Aedes, Anopheles, and Culex genera. From 1,039 contigs of viral origin, we identified 229 viral species infecting mosquitoes, with the orders Picornavirales, Nodamuvirales and Sobelivirales being the most prevalent and abundant. The difference in virome composition was primarily driven by mosquito host species rather than specific collection sites or trap height.ResultsDespite environmental disparities, the virome remained remarkably uniform across different areas of the zoo, emphasizing the strong association between mosquito species and their viral communities. Furthermore, we identified a core virome shared among mosquito species, highlighting potential cross-species transmission events and underscoring the need for targeted surveillance and control measures.ConclusionThese results contribute to our understanding of the interplay between mosquitoes, the environment, and viruses, providing valuable insights for disease intervention strategies in mosquito-borne diseases.
Effect of hyperhomocysteinemia on the prognostic value of triglyceride glucose index in patients with acute coronary syndrome
Authors: Kan Yi , Ma Xiaoteng , Zhao Zehao , Dong Shutong , Xu Yinxiao , Sun Yan , Cheng Yujing , Zhang Dai , Liu Yuyang , Liu Xiaoli , Shi Dongmei , Zhou Yujie 
Published: January 2025

BackgroundThe prognostic value of triglyceride-glucose (TyG) has been well described in patients with coronary artery disease (CAD). Hyperhomocysteinemia (HHcy) promotes insulin resistance and has also been regarded as a potential risk factor for cardiovascular disease. However, the prognostic value of TyG in acute coronary syndrome (ACS) patients undergoing percutaneous coronary intervention (PCI) and the interaction between TyG and HHcy remain unclear.MethodsA total of 1,734 ACS patients undergoing PCI were continuously enrolled between June 2016 and November 2017 at Beijing Anzhen Hospital. Patients were categorized into four groups based on HHcy status and the optimal cut-off value of TyG. The primary endpoint was major adverse cardiovascular events (MACE), a composite of all-cause death, nonfatal myocardial infarction, nonfatal stroke, and unplanned repeat revascularization.ResultsOver a median follow-up of 927 days, 358 patients (20.6%) experienced MACE. The Kaplan-Meier curves showed significant differences in the cumulative incidence of MACE among prespecified groups (p &lt; 0.001). Multivariable Cox regression analysis revealed that higher TyG was significantly associated with an increased risk of MACE in patients without HHcy (HR: 2.36, 95% CI: 1.53–3.64, p &lt; 0.001), but not in patients with HHcy (HR: 1.31, 95% CI: 0.60–2.87, p = 0.503). Restricted cubic splines only demonstrated the prognostic value of TyG in patients without HHcy. A significant interaction was observed for MACE between TyG and HHcy (p for interaction = 0.01).ConclusionsThe prognostic value of TyG was modified by HHcy in ACS patients undergoing PCI. Higher TyG was only associated with an increased risk of MACE in ACS patients without HHcy, but not in ACS patients with HHcy.
Inventory of landslide relics in Zhenxiong County based on human-machine interactive visual interpretation, Yunnan Province, China
Authors: Xue Zhiwen , Xu Chong , Zhang Zhiqiang , Feng Liye , Li Hao , Zhang Hourong , Zhu Dengjie , Sun Jingjing , Wang Peng , Li Lei , Chen Jingyu 
Published: January 2025

IntroductionLandslides occur frequently in Zhenxiong County, posing significant threats to residents’ lives and property. A comprehensive understanding of the development patterns of landslide disasters in this region is crucial for disaster prevention, land-use planning, and risk assessment.MethodsThis study utilized high-resolution satellite imagery from the Google Earth Pro platform and employed a human-machine interactive visual interpretation approach to investigate landslide occurrences. A comprehensive landslide inventory comprising 3,979 landslide outlines was established through extensive literature review and data cleaning techniques. The spatial distribution characteristics and statistical patterns of landslides were analyzed.ResultsThe total landslide-affected area is 319.20 km2, with the largest landslide covering 4.55 km2 and the smallest measuring 1,779 m2. The average landslide area is 80,215 m2, with the majority (73.54%) classified as medium-sized landslides. The landslide area percentage (LAP) is 8.64%, and the landslide number density (LND) is 1.077 landslides per km2, with the highest recorded landslide density being 3.380 landslides per km2. Landslides are predominantly concentrated in four key areas: the confluence of the Baishui River and Yanxi River, Dashuigou Reservoir, both sides of the valley from Heitang Village to Hongyan Village, and Xiaogou Village. These areas are characterized by well-developed water systems, middle and low mountains, and heavily dissected landscapes.DiscussionThe landslide database established in this study provides essential scientific data for analyzing the spatial distribution of landslide disasters in Zhenxiong County. It offers valuable insights for local governments and relevant authorities in disaster prevention, land-use planning, and risk assessment. The findings highlight the significant impact of complex terrain and developed water systems in middle and low mountain regions on landslide disasters. Future studies should further integrate geological and meteorological factors for deeper analysis.
Using data to promote inclusion through early childhood mental health consultation
Authors: Clayback Kelsey A. , Partee Ann M. , Williford Amanda P. , Downer Jason T. , Parker Kyra , Lhospital Ann S. 
Published: January 2025

IntroductionEarly childhood educators continue to need support to build their capacity to promote positive social and emotional development and address challenging behavior when it occurs, without resorting to exclusion. One approach to improve the experiences and outcomes of young children through better support to educators is Early Childhood Mental Health Consultation (ECMHC). ECMHC is an evidence-based intervention that pairs a mental health professional (i.e., “consultant”) with early childhood educators to build the capacity of providers to promote inclusion by supporting young children's social and emotional development.MethodsIn this paper, we describe a model of ECMHC that combines data-driven action planning and an individualized approach to build educators' capacity to implement social and emotional teaching practices with fidelity. The model begins with in-context classroom observations of child engagement and teaching practices, as well as a teacher-report of child behavior. Based on this observational baseline data, a standardized formula selects a personalized set of social and emotional teaching strategies recommended for the focus of consultation, based on the strengths and challenges of the teacher and child. Strategies included foundational strategies (e.g., fostering strong teacher-child relationships), flexible strategies (e.g., using cues and visuals), and targeted strategies (e.g., supporting problem-solving skills). Consultants and teachers then collaboratively work together to select strategies to focus on in consultation, allowing for flexibility and individualization based on individual teacher and child strengths and challenges.ResultsWe address the following two aims: (1) examine teaching practices and children's behavior at the beginning of consultation and how these data resulted in different recommended teaching strategies, based on our standardized formula, (2) present three case examples to further illustrate how these data guided consultation to improve social and emotional teaching practices during the 2023 to 2024 school year. We found that this baseline data collection and the subsequent data-driven process for selecting strategies was feasible in that all consultants and teachers served by ECMHC were able to use it. We also found variability in baseline data and the associated ECMHC teaching strategies recommended, suggesting that the measures were sensitive to unique classroom needs and individualized recommended strategies accordingly.DiscussionWe discuss how this approach allowed consultants to tailor ECMHC services to the unique strengths and challenges of each child and teacher dyad, while being firmly grounded in empirical research and previously validated assessments.
Evaluating the impact of visceral fat on the outcomes of frozen embryo transfer via bioelectrical impedance analysis
Authors: Ni Danyu , Wei Yi , Xie Qijun , Wang Xinyu , Yu Kaidi , Jiang Wei , Yang Ye , Ling Xiufeng 
Published: January 2025

ObjectivesThe increasing prevalence of obesity underscores the need to explore its impact on assisted reproductive technology (ART) outcomes. This study aims to evaluate the association between visceral fat area (VFA), measured by bioelectrical impedance analysis (BIA), and pregnancy outcomes following frozen embryo transfer (FET).MethodsIn this retrospective clinical study, the data of 1,510 patients who underwent FET between April 2022 and April 2023 were analyzed. The VFA was measured by BIA, and patients were categorized into low and high VFA groups based on a threshold of 65 cm². Pregnancy outcomes were compared between the two groups. Univariable and multivariate logistic regression analyses, along with restricted cubic spline (RCS) modeling, were used to adjust for age, body mass index (BMI), and basal estradiol (E2) levels to determine the relationship between VFA and FET outcomes.ResultsThere were significant differences in baseline characteristics and outcomes between the two groups. The high VFA group was characterized by older age and a lower basal estradiol (E2) level. The biochemical pregnancy rate, implantation rate, clinical pregnancy rate (CPR), and live birth rate (LBR) were significantly lower in the high VFA group. Logistic regression revealed a significant negative correlation between the high VFA group and both CPR and LBR. The RCS model demonstrated that the VFA was nonlinearly correlated with CPR and LBR. Subgroup analysis showed that among individuals under 35 years of age or with a BMI &lt; 24, high VFA was significantly associated with poorer CPR and LBR.ConclusionsHigh VFA is associated with poorer pregnancy outcomes after FET in female patients with infertility, with both CPR and LBR decreasing as VFA increases. Clinicians should consider VFA as an important reference for targeted fat management interventions to optimize reproductive success, especially when VFA exceeds 65 cm².
Association between neutrophil-to-lymphocyte ratio and left ventricular diastolic dysfunction in patients with type 2 diabetes mellitus
Authors: Yang Xueyang , Shi Yinze , Zhang Huan , Huang Liying , Zhang Jiaoyue , Min Jie , Chen Lulu 
Published: January 2025

BackgroundDiabetes has become a global pandemic, posing a sustained threat to human health, primarily due to its associated complications. Left ventricular diastolic dysfunction (LVDD) is a prevalent cardiac complication among patients with diabetes. Since most patients are asymptomatic and lack relevant biomarkers, LVDD has not attracted significant attention from clinicians. The neutrophil-to-lymphocyte ratio (NLR) is a widely studied inflammation biomarker that has been suggested to be linked to various medical conditions, including cardiac diseases. However, its association with LVDD among patients with type 2 diabetes mellitus (T2DM) has not been explored.AimTo clarify the relationship between NLR and LVDD among patients with type 2 diabetes.MethodsWe conducted a cross-sectional study using medical records from 855 patients diagnosed with T2DM who were admitted to the Endocrinology department at Wuhan Union Hospital. According to the ASE/EACVI 2016 recommendations, these patients were categorized into two groups based on sonographic parameters: patients with normal left ventricular diastolic function (the non-LVDD group) and patients with LVDD (the LVDD group). NLR values were calculated and divided into three different levels. Statistical analysis was conducted to evaluate the correlation between NLR levels and the prevalence of LVDD.ResultsThe prevalence of LVDD among hospitalized patients with T2DM in our study was 47.8% (409/855). The mean NLR value of the LVDD group was significantly higher compared with the non-LVDD group [1.60 (1.24-2.05) vs 1.85 (1.44-2.31), P&lt;0.001]. The prevalence of LVDD in the three different NLR levels was 35.51% (76/214), 49.27% (203/412), and 56.77% (130/229), respectively. Unjustified logistic analysis showed that NLR levels were positively associated with the prevalence of LVDD (P &lt;0.001). Compared to the low level of NLR, the unadjusted odds ratios (OR) of LVDD at the medium and high levels were 1.764 (1.255-2.478, P=0.001) and 2.384 (1.626-3.497, P&lt;0.001), respectively (P for trend &lt;0.001).ConclusionOur findings suggest that the NLR is a potential indicator for assisting clinicians in identifying LVDD in patients with T2DM. Patients with elevated NLR levels may be at a greater risk of developing LVDD than those with lower NLR levels, which may require attention and interventions to prevent patients from progressing into heart failure.
Analysis of rainwater quality in a quarry site in southwestern Nigeria
Authors: Taiwo Temitope Mary , Ogunbode Timothy Oyebamiji 
Published: January 2025

IntroductionWater has been of paramount importance to humanity throughout history. Understanding the factors contributing to water pollution, particularly those that compromise its quality and sustainability, is essential. This study investigates the impact of quarry activities on the portability of rainwater within a quarry site, focusing on the relationship between quarry operations, heavy metals, and the physicochemical properties of rainwater.MethodsThe study was conducted at Sanlong Quarry Industry in Ifelodun, Southwestern Nigeria. Rainwater samples were collected along a 15–150 m transect within the quarry for analysis of heavy metals and physicochemical contents. Five heavy metals cadmium (Cd), chromium (Cr), zinc (Zn), lead (Pb), and magnesium (Mg) were determined using Atomic Absorption Spectrophotometer (AAS). Additionally, eight physicochemical properties, including electrical conductivity, pH, total solids, suspended solids, total dissolved solids, nitrate, turbidity, and total phosphorus, were analyzed using standard procedures. Variance and correlation analysis of the rainwater’s physicochemical parameters was conducted using Statistical Product for Service Solutions (SPSS).ResultsThe analysis indicated general compliance with water quality standards. However, elevated levels of Pb and Cd in certain areas exceeded WHO, NAFDAC, and US-EPA limits, while Cr, Zn, and Mg were found to have minimal impact on rainwater quality.DiscussionRainwater collected at Sanlong Quarry may not be safe for consumption due to heavy metal contamination, particularly Pb and Cd. This finding underscores the need for alternative clean water sources for both quarry staff and the surrounding community. Quarry management must prioritize the provision of clean water and raise awareness among personnel about the risks associated with consuming contaminated rainwater.
CD56neg CD16+ cells represent a distinct mature NK cell subset with altered phenotype and are associated with adverse clinical outcome upon expansion in AML
Authors: Wlosik Julia , Orlanducci Florence , Richaud Manon , Demerle Clemence , Amara Amira Ben , Rouviere Marie-Sarah , Livrati Philippe , Gorvel Laurent , Hospital Marie-Anne , Dulphy Nicolas , Devillier Raynier , Vey Norbert , Olive Daniel , Chretien Anne-Sophie 
Published: January 2025

IntroductionAcute myeloid leukemia (AML) is a rare haematological cancer with poor 5-years overall survival (OS) and high relapse rate. Leukemic cells are sensitive to Natural Killer (NK) cell mediated killing. However, NK cells are highly impaired in AML, which promote AML immune escape from NK cell immune surveillance. We made the first report of CD56neg CD16+ NK cells expansion in AML. This unconventional subset has been reported to expand in some chronic viral infections. Although it is unclear whether CD56neg NK cells expansion mechanism is common across diseases, it seems more relevant than ever to further investigate this subset, representing a potential therapeutic target.MethodsWe used PBMCs from AML patients and HV to perform mass cytometry, spectral flow cytometry, bulk RNA-seq and in vitro assays in order to better characterize CD56neg CD16+ NK cells that expand in AML.ResultsWe confirmed that CD56neg CD16+ NK cells represent a unique NK cell subset coexpressing Eomes and T-bet. CD56neg CD16+ NK cells could recover CD56 expression in vitro where they displayed unaltered NK cell functions. We previously demonstrated that CD56neg CD16+ NK cells expansion at diagnosis was associated with adverse clinical outcome in AML. Here, we validated our findings in a validation cohort of N=38 AML patients. AML patients with CD56neg CD16+ NK cells expansion at diagnosis had decreased overall survival (HR[CI95]=5.5[1.2-24.5], p=0.0251) and relapse-free survival (HR[CI95]=13.1[1.9-87.5], p=0.0079) compared to AML patients without expansion after 36 months follow-up. RNA-seq unveiled that CD56neg CD16+ NK cells were mature circulating NK cells with functional capacities. Upon expansion, CD56neg CD16+ NK cells from AML patients showed altered proteomic phenotype, with increased frequency of terminally mature CD56neg CD16+ NK cells expressing TIGIT along with decreased frequency of Siglec-7+ CD56neg CD16+ NK cells.DiscussionTaken together, our results suggest that we could harness CD56neg CD16+ NK cells cytotoxic potential in vitro to restore NK cell anti-tumor response in AML patients with CD56neg CD16+ NK cells expansion and improve patients’ prognosis. To conclude, CD56neg CD16+ NK cells represent a relevant target for future NK-cell-based immunotherapies in AML.
Pan-cancer analysis of Arp2/3 complex subunits: focusing on ARPC1A’s role and validating the ARPC1A/c-Myc axis in non-small cell lung cancer
Authors: Zhou Chenkang , Chen Yuxin , Chen Shuhui , Hu Lijuan , Wang Junjun , Wang Yumin 
Published: January 2025

BackgroundThe Arp2/3 complex is a key regulator of tumor metastasis, and targeting its subunits offers potential for anti-metastatic therapy. However, the expression profiles, prognostic relevance, and diagnostic value of its subunits across cancers remain poorly understood. This study aims to investigate the clinical relevance of Arp2/3 complex subunits, particularly ARPC1A, in pan-cancer, and to further analyze the potential biological mechanisms of ARPC1A, as well as its association with immune infiltration and chemotherapy drug sensitivity.MethodsTo explore the differential expression of Arp2/3 complex subunits and their clinical relevance across cancers, we analyzed data from TCGA and GTEx databases. The relationship between ARPC1A and immune infiltration, as well as its interactions with functional proteins, was examined using the TCPA and TIMER2.0 databases. Gene Set Enrichment Analysis (GSEA) was performed to identify ARPC1A-associated signaling pathways. Chemotherapy drug sensitivity correlated with ARPC1A expression was assessed using CellMiner, GDSC, and CTRP databases. The effect of ARPC1A on c-Myc expression was validated by quantitative PCR (qPCR) and Western blot. Finally, the biological role of ARPC1A in non-small cell lung cancer (NSCLC) cells was further validated using CCK-8, EdU incorporation, colony formation, and Transwell assays.ResultsThe Arp2/3 complex subunits, particularly ARPC1A, are frequently overexpressed in a majority of cancers, correlating with poor prognostic outcomes and demonstrating significant diagnostic utility. Copy number variations may play a role in the dysregulation of Arp2/3 complex subunit expression. The small molecule X4.5.dianilinophthalimide has shown promise as a targeted therapeutic agent in a pan-cancer context. Functional predictions indicate that ARPC1A is implicated in oxidative phosphorylation pathways and cell proliferation-related signaling pathways, including those mediated by MYC, with ASNS potentially acting as an upstream regulator. Furthermore, ARPC1A has been implicated in the resistance to chemotherapy drugs, including gefitinib. In vitro experiments corroborate that ARPC1A may enhance malignant phenotypes in non-small cell lung cancer (NSCLC) cells through the regulation of c-Myc expression.ConclusionOur study offers novel insights into targeting Arp2/3 complex subunits as an anti-cancer strategy and underscores the potential of ARPC1A as a novel biomarker for tumor diagnosis, prognosis, and the prediction of immune therapy responses.