bluuebunny/crossref_metadata_2025_split huggingface dataset:
Recombinant cyclin B-Cdk1-Suc1 capable of multi-site mitotic phosphorylationin vitro
Authors: Shintomi Keishi , Masahara-Negishi Yuki , Shima Masami , Tane Shoji , Hirano Tatsuya 
Published: September 2023

AbstractCyclin-dependent kinase 1 (Cdk1) complexed with cyclin B phosphorylates multiple sites on hundreds of proteins during mitosis. However, it is not fully understood how multi-site mitotic phosphorylation by cyclin B-Cdk1 controls the structures and functions of individual substrates. Here we develop an easy-to-use protocol to express recombinant vertebrate cyclin B and Cdk1 in insect cells from a single baculovirus vector and to purify their complexes with excellent homogeneity. A series ofin-vitroassays demonstrate that the recombinant cyclin B-Cdk1 can efficiently and specifically phosphorylate the SP and TP motifs in substrates. The addition of Suc1 (a Cks1 homolog in fission yeast) accelerates multi-site phosphorylation of an artificial substrate containing TP motifs. Importantly, we show that mitosis-specific multi-subunit and multi-site phosphorylation of the condensin I complex can be recapitulatedin vitrousing recombinant cyclin B-Cdk1-Suc1. The materials and protocols described here will pave the way for dissecting the biochemical basis of critical mitotic processes that accompany Cdk1-mediated large-scale phosphorylation.
Forecasting Bitcoin returns using machine learning algorithms: impact of investor sentiment
Authors: Ben Hamadou Fatma , Mezghani Taicir , Zouari Ramzi , Boujelbène-Abbes Mouna 
Published: September 2023

PurposeThis study aims to assess the predictive performance of various factors on Bitcoin returns, used for the development of a robust forecasting support decision model using machine learning techniques, before and during the COVID-19 pandemic. More specifically, the authors investigate the impact of the investor's sentiment on forecasting the Bitcoin returns.Design/methodology/approachThis method uses feature selection techniques to assess the predictive performance of the different factors on the Bitcoin returns. Subsequently, the authors developed a forecasting model for the Bitcoin returns by evaluating the accuracy of three machine learning models, namely the one-dimensional convolutional neural network (1D-CNN), the bidirectional deep learning long short-term memory (BLSTM) neural networks and the support vector machine model.FindingsThe findings shed light on the importance of the investor's sentiment in enhancing the accuracy of the return forecasts. Furthermore, the investor's sentiment, the economic policy uncertainty (EPU), gold and the financial stress index (FSI) are the top best determinants before the COVID-19 outbreak. However, there was a significant decrease in the importance of financial uncertainty (FSI and EPU) during the COVID-19 pandemic, proving that investors attach much more importance to the sentimental side than to the traditional uncertainty factors. Regarding the forecasting model accuracy, the authors found that the 1D-CNN model showed the lowest prediction error before and during the COVID-19 and outperformed the other models. Therefore, it represents the best-performing algorithm among its tested counterparts, while the BLSTM is the least accurate model.Practical implicationsMoreover, this study contributes to a better understanding relevant for investors and policymakers to better forecast the returns based on a forecasting model, which can be used as a decision-making support tool. Therefore, the obtained results can drive the investors to uncover potential determinants, which forecast the Bitcoin returns. It actually gives more weight to the sentiment rather than financial uncertainties factors during the pandemic crisis.Originality/valueTo the authors’ knowledge, this is the first study to have attempted to construct a novel crypto sentiment measure and use it to develop a Bitcoin forecasting model. In fact, the development of a robust forecasting model, using machine learning techniques, offers a practical value as a decision-making support tool for investment strategies and policy formulation.
India’s entrepreneurial landscape: unpacking the relationship between entrepreneurial orientation and business performance
Authors: Singh Bedi Harpreet , Vij Sandeep , Farooq Rayees 
Published: September 2023

Purpose
The aim of this paper is to provide a unique perspective on entrepreneurship by examining how different ways of understanding entrepreneurial orientation (EO) affect business performance (BP). The study uses a five-dimensional approach to understand EO’s relationship with BP.

Design/methodology/approach
A personal survey of key informants (who have decision-making power in their firm), one each from 550 North Indian firms has been conducted. The hypotheses were tested using confirmatory factor analysis and structural equation modeling.

Findings
The results indicate that both uni-dimensional and multi-dimensional conceptualizations of EO are equally valid and have a significant impact on BP. The study highlights the contextual nature of the relationship between EO and BP.

Practical implications
This study supports a comprehensive five-dimensional approach to EO, benefiting researchers and management practitioners. It validates an integrated measurement of BP and advances entrepreneurship theories, enabling broader generalizations for improved decision-making and strategy development.

Originality/value
The study is relevant for researchers and management practitioners. This study supports the five-dimensional conceptualization of EO and reveals the relevance of both uni-dimensional and multi-dimensional conceptualizations of EO. The study also lends support to the integrated approach of BP measurement. The results may also help to generalize entrepreneurship theories.
Design and preliminary evaluation of a lower limb exoskeleton based on hydraulic actuator
Authors: Han Yali , Liu Shunyu , Chang Jiachen , Sun Han , Li Shenyan , Gao Haitao , Jin Zhuangzhuang 
Published: September 2023

Purpose
This paper aims to propose a novel system design and control algorithm of lower limb exoskeleton, which provides walking assistance and load sharing for the wearer.

Design/methodology/approach
In this paper, the valve-controlled asymmetrical hydraulic cylinder is selected for driving the hip and knee joint of exoskeleton. Pressure shoe is developed that purpose on detecting changes in plantar force, and a fuzzy recognition algorithm using plantar pressure is proposed. Dynamic model of the exoskeleton is established, and the sliding mode control is developed to implement the position tracking of exoskeleton. A series of prototype experiments including benchtop test, full assistance, partial assistance and loaded walking experiments are set up to verify the tracking performance and power-assisted effect of the proposed exoskeleton.

Findings
The control performance of PID control and sliding mode control are compared. The experimental data shows the tracking trajectories and tracking errors of sliding mode control and demonstrate its good robustness to nonlinearities. sEMG of the gastrocnemius muscle tends to be significantly weakened during assisted walking.

Originality/value
In this paper, a structure that the knee joint and hip joint driven by the valve-controlled asymmetrical cylinder is used to provide walking assistance for the wearer. The sliding mode control is proposed to deal with the nonlinearities during joint rotation and fluids. It shows great robustness and frequency adaptability through experiments under different motion frequencies and assistance modes. The design and control method of exoskeleton is a good attempt, which takes positive impacts on the productivity or quality of the life of wearers.
Hosting an international conference on multiculturalism in an Arab Israeli teachers’ college: a reflection on personal and organizational learning
Authors: Khair Abbas Randa 
Published: September 2023

Purpose
Conferences, while central to academic endeavors, are an understudied research site. This purpose of this study is to describe the experience of an Arab Israeli teachers’ college in hosting a large international academic conference on multiculturalism, conducted entirely in English. Most of the academic staff and all of the students are Arabic speakers. This added a layer of challenge for all our faculty and students.

Design/methodology/approach
This reflective, retroactive and descriptive case study builds on the above points. It is a reflective “case story” told by the college head.

Findings
This paper describes the benefits and growth experienced by each of the involved parties, which resulted in a changing academic culture and new academic self-confidence and vision for all concerned. This paper also details weaknesses and opportunities missed. Envisioning, planning and bringing to fruition this conference was a crucible experience for the college, enhancing our multicultural understanding, learning and commitment.

Originality/value
This reflective description offers an original perspective on multiculturalism through the lens of an international conference hosted by an academic institution where the native language is not English.
Cutting the clutter: how minimalism drives customer citizenship behaviour within the sharing economy-based services
Authors: Rasheed A.K. Fazeen , Balakrishnan Janarthanan 
Published: September 2023

PurposeUsing the stimulus-organism-response (SOR) theory, this study explores the effect of religiosity, resource sharing and environmental attitudes on minimalist behaviour in sharing economy-based services and its subsequent influence on customer citizenship behaviour.Design/methodology/approachThis research employed a descriptive, quantitative and cross-sectional design, examining data from 530 Indian tourists who used sharing economy-based services. The proposed conceptual model was evaluated through partial least squares-structural equation modelling (PLS-SEM), and the impact of control variables was examined via PLS-multi-group analysis (PLS-MGA).FindingsThe study findings validated that religiosity, resource sharing and environmental attitudes significantly foster minimalist behaviour in sharing economy-based services. This minimalist behaviour, in turn, positively impacts customer citizenship behaviour, affecting aspects such as tolerance, feedback, advocacy and assistance to others. The model's outcomes also indicated the substantial moderating roles of age and gender.Originality/valueThis study is novel in its exploration of how religiosity, resource sharing and environmental attitudes as stimuli can shape minimalist behaviour within sharing economy-based services and further influence customer citizenship behaviour. By uncovering the significant roles of age and gender in this context, the research offers unique insights into understanding the dynamics of consumer behaviour within the burgeoning sharing economy. The findings provide valuable direction for stakeholders in the sharing economy and policymakers aiming to cultivate positive consumer behaviours and advance the sector's sustainability.
Development and characterization of composite materials with multi-walled carbon nanotubes and graphene nanoplatelets for powder bed fusion
Authors: Lopes Ana C. , Sampaio Álvaro M. , Pontes António J. 
Published: September 2023

Purpose
With the technological progress, high-performance materials are emerging in the market of additive manufacturing to comply with the advanced requirements demanded for technical applications. In selective laser sintering (SLS), innovative powder materials integrating conductive reinforcements are attracting much interest within academic and industrial communities as promising alternatives to common engineering thermoplastics. However, the practical implementation of functional materials is limited by the extensive list of conditions required for a successful laser-sintering process, related to the morphology, powder size and shape, heat resistance, melt viscosity and others. The purpose of this study is to explore composite materials of polyamide 12 (PA12) incorporating multi-walled carbon nanotubes (MWCNT) and graphene nanoplatelets (GNP), aiming to understand their suitability for advanced SLS applications.

Design/methodology/approach
PA12-MWCNT and PA12-GNP materials were blended through a pre-optimized process of mechanical mixing with various percentages of reinforcement between 0.50 wt.% and 3.00 wt.% and processed by SLS with appropriate volume energy density. Several test specimens were produced and characterized with regard to processability, thermal, mechanical, electrical and morphological properties. Finally, a comparative analysis of the performance of both carbon-based materials was performed.

Findings
The results of this research demonstrated easier processability and higher tensile strength and impact resistance for composites incorporating MWCNT but higher tensile elastic modulus, compressive strength and microstructural homogeneity for GNP-based materials. Despite the decrease in mechanical properties, valuable results of electrical conductivity were obtained with both carbon solutions until 10–6 S/cm.

Originality/value
The carbon-based composites developed in this research allow for the expansion of the applicability of laser-sintered parts to advanced fields, including electronics-related industries that require functional materials capable of protecting sensitive devices against electrostatic discharge.
A collective decision-making model of p2p lending platforms compared to bank lending
Authors: Ben-Yashar Ruth , Krausz Miriam 
Published: September 2023

Purpose
This study aims to develop a theoretical model that uses the decision-making theory in a financial intermediation setting to provide insights into the differences between the outcomes of the decision-making process for a bank and for a peer-to-peer (p2p) lending platform to explain the role of p2p lending versus bank lending in the credit market.

Design/methodology/approach
This study develops a novel approach to explaining the differences between p2p lending and bank lending by using the decision-making theory. In particular, it analyzes the likelihood of a risky borrower being able to obtain a loan from a p2p lending platform versus the likelihood of being able to obtain a loan from a bank. The results contribute a theoretical understanding of factors that can determine the role of p2p lending platforms versus that of banks in the credit market, with implications for recovery from an economic crisis.

Findings
p2p lending platforms have the potential for contributing to economic recovery when they are subject to less regulations and are able to offer a faster and less costly lending process than do banks and when they are used by a large number of lenders. However, the potential role of p2p lending platforms in recovery might be reduced when banks have access to anticyclical measures that reduce banks’ capital requirements or provide them with low-cost funds.

Originality/value
This study provides a novel approach to explaining the differences between p2p lending and bank lending by using the decision-making theory. The results contribute a theoretical understanding of factors that can determine the role of p2p lending platforms versus that of banks in the credit market.
The accessibility of beaches for blind people and their guide dogs: accessible tourism and inclusion in Spain
Authors: Florido-Benítez Lázaro 
Published: September 2023

Purpose
The purpose of this research is to analyse the accessibility of Spanish beaches for blind people and their guide dogs as an inclusive tourist destination for national and international tourists.

Design/methodology/approach
This empirical research used a quantitative and qualitative methodology from public and private organizations, which intends to give voice to blind people to improve their quality of life.

Findings
This study presents a new and surprising contribution to the issue of accessibility of beaches for blind people and future studies related to this topic. Findings revealed that Spain has only nine adapted beaches for blind people, and most Spanish tourist destinations exclude blind people and its guide dogs from enjoying the beach and the sun and their facilities. In Spain there are in total 3,547 beaches; 10.3% of these are adapted beaches for people with reduced mobility, whereas only 0.25% are adapted beaches for blind people. Indeed, results also suggest that more efforts are required in response to the needs of blind people in tourism and transport activities by researchers, academics and government bodies.

Originality/value
The contribution of this paper is especially important for the management of the accessibility of Spanish beaches for blind people and their guide dogs by public and private organizations.
Neighbours matter and the weak succumb: Ash dieback infection is more severe in ash trees with fewer conspecific neighbours and lower prior growth rate
Authors: Cracknell David J. , Peterken George F. , Pommerening Arne , Lawrence Peter J. , Healey John R. 
Published: September 2023

AbstractThe epidemiology and severity of ash dieback (ADB), the disease caused by the ascomycete fungusHymenoscyphus fraxineus, has been linked to a variety of site conditions; however, there has been a lack of analysis at an individual tree scale.Symptoms of ADB were scored on ca. 400 trees ofFraxinus excelsior(ash) in permanent sample plots during two successive years in a UK natural woodland reserve. Using comprehensive plot records maintained since 1945, and detailed spatial records updated since 1977, we assembled an array of potential explanatory variables, including site environment factors, ash tree density, previous and present tree condition and near neighbourhood summary statistics (NNSS), such as species mingling and size dominance. Their impact on the severity of ADB of focal ash trees was tested with generalised linear mixed effects models (GLMM).The severity of ADB was much greater in the lower slope parts of the site with moister soils and least in a managed area subject to tree thinning in the previous 35 years. Severity of ADB had a negative association with focal ash tree prior relative growth rate over a period of a decade immediately before the disease was detected at the site. Greater ADB severity was also significantly associated with smaller diameter at breast height of ash trees. Additionally, ADB was significantly positively associated with a greater proportion of heterospecific trees amongst the six nearest neighbours of the focal tree.Synthesis.The relationship of the severity of ADB disease with site environment, tree condition and neighbourhood is complex but nevertheless important in the progression of the disease. The findings suggest some silvicultural interventions, such as thinning to increase the vigour of retained ash trees, might reduce the impact of ADB.
Droplet‐based optical trapping for cell separation in mock forensic samples
Authors: Valle Michael , O'Brien Benjamin , Green Tracey Dawson , Reiner Joseph E. , Seashols‐Williams Sarah 
Published: September 2023

AbstractOptical tweezers have a wide range of uses for mechanical manipulation of objects in the microscopic range. This includes both living and static cells in a variety of biomedical and research applications. Single‐focus optical tweezers, formed by focusing a laser beam through a high numerical aperture immersion objective, create a significant force, which enables controlled transport of a variety of different cell types and morphologies in three dimensions. Optical tweezers have been previously reported to capture and separate spermatozoa from a reconstituted simulated postcoital sample. We report herein the development of a simplified, more efficient cell transfer protocol that can separate and isolate both spermatozoa as well as leukocytes, with similar efficiencies as those previously reported. The new cell transfer method was used to separate sperm cells from a reconstituted mixture of spermatozoa and vaginal epithelial cells, with complete STR profiles developed from 50 cells with little evidence of contribution from the female contributor to the mixture. This modified protocol was then used to separate 21 samples of enriched leukocytes, with trapped cells ranging from 5 to 22 cells. Complete STR profiles were developed from as few as 10 leukocytes. Thus, with minimal sample preparation and a short trapping time, this method has the potential to provide an alternative to traditional differential extraction methods for separation of sperm:nonsperm mixtures while also providing versatility for separation of cells with differing morphologies.
Toward organizational integrity measurement: Developing a theoretical model of organizational integrity
Authors: Fuerst Madeleine J. , Luetge Christoph , Max Raphael , Kriebitz Alexander 
Published: September 2023

AbstractOrganizational integrity is a key concept with and through which a company can assume its responsibility for ethical and societal issues. It is a basic premise for sustainable corporate success, as ethical risks ultimately become economic risks for a company. Recent research shows the potential of integrity‐based governance models to reduce corporate risks and to improve business performance. However, companies are not yet able to assess nor evaluate their level of organizational integrity in a sound and systematic way. We aim to develop a theoretical model as a basis for the measurement of organizational integrity by conceptualizing the construct and sizing the theoretical model's scope. We suggest that the theoretical model follows a holistic approach and involves three types of dimensions: prerequisite dimensions, independent dimensions, and dependent dimensions. The organizational integrity triad—consisting of active commitments to self‐imposed norms and principles, their transparent institutionalization into corporate processes and structures, and their implementation into action—plays a key role in this context.
How does founders' religiosity affect the proactive environmental strategies in family firms? Evidence from China
Authors: Yang Xuelei , Shang Hangbiao , Lan Hailin , Li Weining 
Published: September 2023

AbstractThe current literature expands the existing knowledge of the antecedents of proactive environmental strategies (PES) in family firms from the perspectives of institutional, market and family involvement logics. However, scholars have not considered the influence of key family decision‐makers and their characteristics in this regard. Based on the ability and willingness framework, this study focuses on how founders' religiosity affects family firms' PES. Using data from the 2010 Chinese Private Enterprise Survey conducted by Chinese officials, we found that founders' religiosity drives family firms to implement PES. Altruistic and long‐term orientation are the internal mechanisms of this effect. Founders' political status enhances the positive impact of their religiosity on PES, whereas the founders' need for socioemotional wealth protection weakens the relationship between founders' religiosity and family firms' PES. We also found that Eastern and Western founders' religiosity has an asymmetric effect on family firms' PES. This study contributes to the literature on the PES of family firms, the family firm's ability and willingness framework, and family business heterogeneity.