bluuebunny/crossref_metadata_2025_split huggingface dataset:
A circulating microRNA panel as a novel dynamic monitor for oral squamous cell carcinoma
Authors: Piao Yudan , Jung Seung-Nam , Lim Mi Ae , Oh Chan , Jin Yan Li , Kim Hae Jong , Nguyen Quoc Khanh , Chang Jae Won , Won Ho-Ryun , Koo Bon Seok 
Published: February 2023

AbstractOral squamous cell carcinoma (OSCC) has high recurrence and mortality rates despite advances in diagnosis and treatment. Therefore, it is necessary to identify new biomarkers for early detection, efficient monitoring, and prognosis prediction. Since microRNA (miRNA) is stable and detectable in serum, it has been reported to inform the diagnosis and monitor disease progression through liquid biopsy. In this study, a circulating specific miRNA panel in OSCC patients was developed, and its usefulness as
a dynamic monitor was validated. Small RNAs were extracted from the serum of OSCC patients (n = 4) and normal controls (n = 6) and profiled using next-generation sequencing. NGS identified 42 differentially expressed miRNAs (DEmiRNAs) in serum between patients with OSCC and healthy controls, with threefold differences (p < 0.05). Combining the 42 DEmiRNAs and The Cancer Genome Atlas (TCGA) databases OSCC cohort, 9 overlapping DEmiRNAs were screened out. Finally, 4 significantly up-regulated miRNAs (miR-92a-3p, miR-92b-3p, miR-320c and miR-629-5p) were identified from OSCC patients via validation in the Chungnam National University Hospital cohort. Application of the specific miRNA panel for distinguishing OSCC patients from healthy controls produced specificity and sensitivity of 97.8 and 74%, respectively. In addition, the serum levels of these 4 miRNAs significantly decreased after complete surgical resection and increased after recurrence. We suggest that circulating 4-miRNA panel might be promising non-invasive predictors for diagnosing and monitoring the progression of patients with OSCC.
Dynamics of fatty acid and non-volatile polar metabolite profiles in colostrum and milk depending on the lactation stage and parity number of sows
Authors: Settachaimongkon Sarn , Homyog Kunaporn , Mekboonsonglarp Wanwimon , Soonoue Pitikorn , Lerdamnuaylarp Theerawat , Prayoonpeeraput Poonradit , Theil Peter Kappel , Nuntapaitoon Morakot 
Published: February 2023

AbstractThe objective of this study was to investigate the impact of lactation stage and parity number on fatty acid and non-volatile polar metabolite profiles in sow colostrum and milk using a metabolomics approach. A total number of 63 colostrum, transient and mature milk were collected from primiparous and multiparous Landrace × Yorkshire crossbred sows. Macrochemical, fatty acid and non-volatile polar metabolite compositions of samples were analyzed using infrared spectrometry, gas chromatography coupled with mass-spectrometry and proton nuclear magnetic resonance spectroscopy, respectively. Univariate and multivariate statistical analysis demonstrated significant impacts of lactation stage and parity number on colostrum and milk compositions. Chemometric analysis revealed significant influences of sow parity on the distinction in fatty acid profiles of mature milk while the distinction in non-volatile polar metabolite profiles was more evident in colostrum. Alterations in the concentration of linoleic (C18:2n6), lignoceric (C24:0), behenic (C22:0), caprylic (C8:0) and myristoleic (C14:1) acid together with those of creatine, creatinine phosphate, glutamate and glycolate were statistically suggested to be mainly affected by sow parity number. Variations in the concentration of these compounds reflected the physiological function of sow mammary gland influenced. This information could be applied for feed and feeding strategies in lactating sows and improving lactating performances.
Differentially expression analyses in fruit of cultivated and wild species of grape and peach
Authors: Huang Weijian , Li Meng , Zhang Huangwei , Wu Juyou , Dunwell Jim M. , Zhang Shaoling 
Published: February 2023

AbstractThrough agronomic traits and sequencing data, the cultivated and wild varieties of grapes and peaches were analyzed and compared in terms of fruit size, fruit flavor, fruit resistance, and fruit color. Cultivated grapes and peaches have advantages in fruit size, soluble sugar content, sugar and acid ratio, etc. Wild grapes and peaches have utility value in resistance. The results showed that there were 878 and 301 differentially expressed genes in cultivated and wild grapes and peaches in the three growth stages, respectively based on the next-generation sequencing study. Ten and twelve genes related to the differences between cultivated and wild grapes and peaches were found respectively. Among them, three genes, namely chalcone synthase (CHS), glutathione S-transferase (GST) and malate dehydrogenase (MDH1) were present in both cultivated and wild grapes and peaches.
TEMPRANILLO homologs in apple regulate flowering time in the woodland strawberry Fragaria vesca
Authors: Dejahang Ata , Maghsoudi Naeimeh , Mousavi Amir , Farsad-Akhtar Nader , Matias-Hernandez Luis , Pelaz Soraya , Folta Kevin , Mahna Nasser 
Published: February 2023

AbstractThe long juvenile period of fruit trees makes their breeding costly and time-consuming. Therefore, flowering time engineering and shortening the juvenile phase have become a breeding priority for the genetic improvement of fruit tree crops. Many economically valuable fruit trees belong to theRosaceaefamily including apples and strawberries.TEMPRANILLO(TEM) acts as a key player in flowering time control through inhibitingFTfunction. Two genes with high sequence similarity with theArabidopsis TEMgenes were isolated from apple (Malus domestica). Due to the complexity of carrying out functional studies in apple, we characterized their function in woodland strawberry as well as their expression in apple. The expression ofMdTEMgenes in apple tissues from juvenile plants was dramatically higher than that in the tissues from adult trees. In woodland strawberry, the overexpression ofMdTEMgenes down-regulatedFvFT1,FvGA3OX1, andFvGA3OX2genes in strawberry. TheMdTEM-overexpressing lines exhibited delayed flowering, in terms of days to flowering and the number of leaves at flowering. While,RNAi-mediatedsilencing ofTEMresulted in five days earlier flowering, with a lower number of leaves, a higher trichome density, and in some cases, caused in vitro flowering. According to these results and in silico analyses, it can be concluded thatMdTEM1andMdTEM2can be considered as orthologs ofFvTEMand probablyAtTEMgenes, which play an important role in regulating the juvenile phase and flowering time through regulatingFTand GA biosynthetic pathway.
Low frequency electrical waves in ensembles of proteinoid microspheres
Authors: Mougkogiannis Panagiotis , Adamatzky Andrew 
Published: February 2023

AbstractProteinoids (thermal proteins) are produced by heating amino acids to their melting point and initiation of polymerisation to produce polymeric chains. Amino acid-like molecules, or proteinoids, can condense at high temperatures to create aggregation structures called proteinoid microspheres, which have been reported to exhibit strong electrical oscillations. When the amino acids L-glutamic acid (L-Glu) and L-aspartic acid (L-Asp) were combined with electric fields of varying frequencies and intensities, electrical activity resulted. We recorded electrical activity of the proteinoid microspheres’ ensembles via a pair of differential electrodes. This is analogous to extracellular recording in physiology or EEG in neuroscience but at micro-level. We discovered that the ensembles produce spikes of electrical potential, an average duration of each spike is 26 min and average amplitude is 1 mV. The spikes are typically grouped in trains of two spikes. The electrical activity of the ensembles can be tuned by external stimulation because ensembles of proteinoid microspheres can generate and propagate electrical activity when exposed to electric fields.
Graphene-based optofluidic tweezers for refractive-index and size-based nanoparticle sorting, manipulation, and detection
Authors: Gholizadeh Elnaz , Jafari Behnam , Golmohammadi Saeed 
Published: February 2023

AbstractThis work proposes a novel design composed of graphene nanoribbons-based optofluidic tweezers to manipulate and sort bio-particles with radii below 2.5 nm. The suggested structure has been numerically investigated by the finite difference time domain (FDTD) method employing Maxwell's stress tensor analysis (MST). The finite element method (FEM) has been used to obtain the electrostatic response of the proposed structure. The tweezer main path is a primary channel in the center of the structure, where the microfluidic flow translates the nanoparticle toward this channel. Concerning the microfluid's drag force, the nanoparticles tend to move along the length of the main channel. The graphene nanoribbons are fixed near the main channel at different distances to exert optical forces on the moving nanoparticles in the perpendicular direction. In this regard, sub-channels embedding in the hBN layer on the Si substrate deviate bio-particles from the main path for particular nanoparticle sizes and indices. Intense hotspots with electric field enhancements up to 900 times larger than the incident light are realized inside and around the graphene ribbons. Adjusting the gap distance between the graphene nanoribbon and the main channel allows us to separate the individual particle with a specific size from others, thus guiding that in the desired sub-channel. Furthermore, we demonstrated that in a structure with a large gap between channels, particles experience weak field intensity, leading to a low optical force that is insufficient to detect, trap, and manipulate nanoparticles. By varying the chemical potential of graphene associated with the electric field intensity variations in the graphene ribbons, we realized tunability in sorting nanoparticles while structural parameters remained constant. In fact, by adjusting the graphene Fermi level via the applied gate voltage, nanoparticles with any desired radius will be quickly sorted. Moreover, we exhibited that the proposed structure could sort nanoparticles based on their refractive indices. Therefore, the given optofluidic tweezer can easily detect bio-particles, such as cancer cells and viruses of tiny size.
Gut microbial signature in lung cancer patients highlights specific taxa as predictors for durable clinical benefit
Authors: Haberman Yael , Kamer Iris , Amir Amnon , Goldenberg Sapir , Efroni Gilat , Daniel-Meshulam Inbal , Lobachov Anastasiya , Daher Sameh , Hadar Rotem , Gantz-Sorotsky Hadas , Urban Damien , Braun Tzipi , Bar Jair 
Published: February 2023

AbstractWe aimed to determine microbial signature linked with lung cancer (LC) diagnosis and to define taxa linked with durable clinical benefit (DCB) of advanced LC patients. Stool samples for microbial 16S amplicon sequencing and clinical data were collected from 75 LC patients (50 of which were treated with checkpoint inhibitors) and 31 matched healthy volunteers. We compared LC to healthy controls and patients with DCB to those without. LC patients had lower α-diversity and higher between-subject diversity. Random Forests model to differentiate LC cases from controls ROC-AUC was 0.74. Clostridiales, Lachnospiraceae, and Faecalibacterium prausnitzii taxa abundance was decreased in LC compared to controls. High Akkermansia muciniphila correlated with DCB (HR 4.26, 95% CI 1.98–9.16), not only for the immunotherapy-treated patients. In addition, high Alistipes onderdonkii (HR 3.08, 95% CI 1.34–7.06) and high Ruminococcus (HR 7.76, 95% CI 3.23–18.65) correlated with DCB.Our results support the importance of gut microbiome in LC. We have validated the apparent predictive value of Akkermansia muciniphila, and highlighted Alistipes onderdonkii and Ruminococcus taxa correlation with DCB. Upon additional validations those can be used as biomarkers or as targets for future therapeutic interventions.
Simulation-based architecture of a stable large-area $$JDBD$$ atmospheric plasma source
Authors: Roshan M. V. , Razaghi S. , Singh A. 
Published: February 2023

AbstractUnified jet-DBD design, $$JDBD$$

JDBD

, proposed in this work presents large-scale plasma in an unbounded region of atmospheric air, without any need for the flow of gas, offering efficient exposure to sizable and complex objects. This is a simulation-based architecture for stable non-thermal plasma source with notable experimental results. $$JDBD$$

JDBD

geometry optimizes the electric field and charge distribution for a diffuse discharge in the steady air by a key design parameter of $$efficient \; insulation$$

e
f
f
i
c
i
e
n
t

i
n
s
u
l
a
t
i
o
n

. Teflon insulator with a thickness $${d}_{Tf}\ge 10 \; \text{mm}$$

d

Tf

≥
10

mm

imposes an intense and uniform electric field shaped up at the open area in front of the device and generates radially/axially expanded plasma jet. In the $$JDBD$$

JDBD

, phase shift increases by $${I}_{rms}$$

I

rms

and the plasma generates more power than the classical plasma jet. Two distinct states of $$JDBD$$

JDBD

operation indicate the mode-swap at $$0.8\; \text{mA}$$

0.8

mA

and power dissipation. In the reactive $$JDBD$$

JDBD

scheme even small changes in the phase angle effectively improves the electric power.
Comparison of [18F]FIMP, [11C]MET, and [18F]FDG PET for early-phase assessment of radiotherapy response
Authors: Nozaki Satoshi , Nakatani Yuka , Mawatari Aya , Shibata Nina , Hume William E. , Hayashinaka Emi , Wada Yasuhiro , Doi Hisashi , Watanabe Yasuyoshi 
Published: February 2023

AbstractSeveral limitations of [18F]FDG have been reported, such as nonspecific uptake of inflammation foci. Moreover, [11C]MET has been found to accumulate in normal and inflammatory tissues as well as tumors. To increase specificity to tumor tissues, PET probes with tumor-specific molecular targets have been actively developed. [18F]FIMP was found to be highly accumulated in LAT1-positive tumors but not in inflamed tissue. The aim of this study was to explore whether [18F]FIMP can be used for the early-phase evaluation of radiotherapy accompanied by inflammation, and compare its effectiveness with those of [11C]MET and [18F]FDG. Tumor uptake of [18F]FIMP decreased at day 1 after irradiation, and remained low until day 14. Comparatively, that of [18F]FDG initially decreased at day 3 but was transiently elevated at day 7 and then decreased again at day 10. Decreased tumor uptake of [11C]MET was observed at day 10. In line with the uptake of [18F]FIMP, the ratio of Ki-67 immuno-positive cells in tumor tissues significantly decreased at day 1, 7, and 10 as compared with that in the control. These findings suggest that [18F]FIMP may be a PET probe involved in the early detection and prediction of radiotherapy efficacy, although further clarification is needed.
Emotional and behavioral changes in French children during the COVID-19 pandemic: a retrospective study
Authors: Landman Benjamin , Cohen Alicia , Khoury Elie , Trebossen Vincent , Bouchlaghem Nesrine , Poncet-Kalifa Hélène , Acquaviva Eric , Lefebvre Aline , Delorme Richard 
Published: February 2023

AbstractCOVID-19 outbreak caused severe disruptions in daily life, partly due to limitations implemented to prevent the spreading. In France, it included school closures during a national lockdown, then a reopening of schools, with access depending on viral status of students and teachers. Those changes had an impact on children's mental health. We conducted an online cross-sectional study using a parental self-administered survey in December 2021 to explore the emotional and behavioral changes (EBC) during this 5th wave (W5) and retrospectively since the first one (W1) in their children and their multidimensionality with principal factor analysis (PCA) and stability analysis. Out of 4552 parent responders, 62.4% (n = 2839) noticed negative EBC during W1 and 54.1% (n = 2462) during W5 of the pandemic. Only 10.0% of the responders noticed negative EBC at W1 but not during the W5. In younger children (3–6 years old) with significant EBC, PCA revealed three main dimensions at W1 and W5: restlessness, depression and anxiety. In older children (7–13 years old), PCA showed partially similar dimensions: depression-suicidality, anxiety and withdrawal. Almost all correlations between dimensions at W1 and W5 were significantly positive. Every EBC was stable across waves, except for one. Recall bias concerning the EBC during W1 and lack of data concerning parental mental health should be taken into account. Our stability analysis found a strong correlation between dimensions at W1 and W5. Our results highlighted the impact of the COVID-19 outbreak on children's mental health and the predictive aspect of its early deterioration.
The effect of mindfulness-based intervention on neurobehavioural functioning and its association with white-matter microstructural changes in preterm young adolescents
Authors: Siffredi Vanessa , Liverani Maria Chiara , Van De Ville Dimitri , Freitas Lorena G. A. , Borradori Tolsa Cristina , Hüppi Petra Susan , Ha-Vinh Leuchter Russia 
Published: February 2023

AbstractVery preterm (VPT) young adolescents are at high risk of executive, behavioural and socio-emotional difficulties. Previous research has shown significant evidence of the benefits of mindfulness-based intervention (MBI) on these abilities. This study aims to assess the association between the effects of MBI on neurobehavioral functioning and changes in white-matter microstructure in VPT young adolescents who completed an 8-week MBI program. Neurobehavioural assessments (i.e., neuropsychological testing, parents- and self-reported questionnaires) and multi-shell diffusion MRI were performed before and after MBI in 32 VPT young adolescents. Combined diffusion tensor imaging (DTI) and neurite orientation dispersion and density imaging (NODDI) measures were extracted on well-defined white matter tracts (TractSeg). A multivariate data-driven approach (partial least squares correlation) was used to explore associations between MBI-related changes on neurobehavioural measures and microstructural changes. The results showed an enhancement of global executive functioning using parent-reported questionnaire after MBI that was associated with a general pattern of increase in fractional anisotropy (FA) and decrease in axonal dispersion (ODI) in white-matter tracts involved in executive processes. Young VPT adolescents with lower gestational age at birth showed the greatest gain in white-matter microstructural changes after MBI.
Narrowing the coordination solution space during motor learning standardizes individual patterns of search strategy but diversifies learning rates
Authors: Komar John , Seifert Ludovic , Vergne Nicolas , Newell Karl M. 
Published: February 2023

AbstractConstraints on practice can benefit motor learning by guiding the learner towards efficient coordination patterns, but can also narrow the potential solution space of coordination and control. The aim of this paper was to investigate whether narrowing the solution space through more restrictive task constraints limits the expression of potential exploratory behaviours during the learning process, identified using Drifting Markov Models. In a breaststroke swimming task, the change in interlimb coordination of 7 learners practicing for 16 lessons over 2 months was analysed to quantify motor exploration and identify periods of metastable regimes of coordination. Results showed that the observed exploratory dynamics were highly individual both in terms of range of exploration and in the patterns of search. The more restrictive task constraints did not impair the amount of exploration but rather channelled the exploration around a few selected patterns. In addition, restraining the nature of the exploratory process increased the inter-individual differences of the learning rate. Although manipulating the task constraints during learning can help learners to escape from the behavioural consequences of their intrinsic dynamics, maintaining a broad solution space for a diversity of coordination patterns to emerge was key to fostering effective exploration of individual coordination solutions.
Influence of pneumatization on morphology of temporal bone-related vasculatures and their morphometric relationship with ear regions: a computed tomography study
Authors: Aladeyelu Okikioluwa Stephen , Olojede Samuel Oluwaseun , Lawal Sodiq Kolawole , Mbatha Wonder-Boy Eumane , Sibiya Andile Lindokuhle , Rennie Carmen Olivia 
Published: February 2023

AbstractAnatomical variations in the location and position of temporal bone-related vasculature are routinely encountered in clinical practice, contributing to clinical syndromes and complexities in ear-related and neurological surgeries. Pneumatization of the temporal bone (TB) is one of several factors that have been hypothesized to influence the variabilities and variations of these vessels. This study aimed to investigate the association between the degree of pneumatization and the morphologies of some TB-related vessels, as well as their morphometrical relationship with ear regions. Observational retrospective chart review of 496 TBs computed tomographic scans were examined. Different degrees of pneumatization were observed, with hyper-pneumatization being the most common and hypo-pneumatization being the least. Various anatomical variants of the sigmoid sinus (SS), jugular bulb (JB), and internal carotid artery (ICA) were observed. Distances of SS and JB to ear regions were observed to have significant differences (p < 0.05) in laterality. These distances increased relative to increased air cells, showing a significant association (p < 0.05). A significant association (p < 0.001) was also observed between the degree of pneumatization and variants of JB and ICA. High JB, JB dehiscence, and ICA dehiscence were significantly associated with increased pneumatization, while flat JB was significantly associated with decreasing pneumatization. However, no significant association (p = 0.070, p = 0.645) was observed between the degree of pneumatization and morphologies of SS. This study concludes that the degree of pneumatization influences only the jugular bulb variants and ICA dehiscence, as well as the distances of SS and JB to ear regions.
Tissue-resident macrophages are major tumor-associated macrophage resources, contributing to early TNBC development, recurrence, and metastases
Authors: Hirano Ryuichiro , Okamoto Koki , Shinke Miyu , Sato Marika , Watanabe Shigeaki , Watanabe Hitomi , Kondoh Gen , Kadonosono Tetsuya , Kizaka-Kondoh Shinae 
Published: February 2023

AbstractTriple-negative breast cancer (TNBC) is an aggressive and highly heterogenous disease with no well-defined therapeutic targets. Treatment options are thus limited and mortality is significantly higher compared with other breast cancer subtypes. Mammary gland tissue-resident macrophages (MGTRMs) are found to be the most abundant stromal cells in early TNBC before angiogenesis. We therefore aimed to explore novel therapeutic approaches for TNBC by focusing on MGTRMs. Local depletion of MGTRMs in mammary gland fat pads the day before TNBC cell transplantation significantly reduced tumor growth and tumor-associated macrophage (TAM) infiltration in mice. Furthermore, local depletion of MGTRMs at the site of TNBC resection markedly reduced recurrence and distant metastases, and improved chemotherapy outcomes. This study demonstrates that MGTRMs are a major TAM resource and play pivotal roles in the growth and malignant progression of TNBC. The results highlight a possible novel anti-cancer approach targeting tissue-resident macrophages.