bluuebunny/crossref_metadata_2025_split huggingface dataset:
HNF4G increases cisplatin resistance in lung adenocarcinoma via the MAPK6/Akt pathway
Authors: Liang Jiaqi , Zhao Guangyin , Bian Yunyi , Bi Guoshu , Sui Qihai , Zhang Huan , Shi Haochun , Shan Guangyao , Huang Yiwei , Chen Zhencong , Wang Lin , Zhan Cheng 
Published: March 2023

Background
Lung adenocarcinoma is one of the most common tumors, and cisplatin is frequently used in treating lung adenocarcinoma patients. This study aimed to look into the roles and mechanisms of HNF4G in cisplatin resistance of lung adenocarcinoma.

Materials & Methods
Cisplatin resistance and gene expression data of 542 cell lines from the CTRP and CCLE databases were analyzed. HNF4G expression was detected in the lung adenocarcinoma cell lines after treatment with various concentrations of cisplatin. Cisplatin sensitivity curves were detected in cells that overexpressed or knocked down HNF4G. The ChIP-Seq data were then analyzed to identify the targets of HNF4G involved in cisplatin resistance. Expression and phosphorylation of the MAPK6/Akt pathway were detected after HNF4G was overexpressed or knocked down. Finally, ChIP-qPCR and dual-luciferase assays were used to investigate the regulation of HNF4G on MAPK6.

Results
In cell lines, high expression of HNF4G was significantly positively correlated with cisplatin resistance, and lung adenocarcinoma patients who had high HNF4G expression had a poor prognosis. Cisplatin treatment increased HNF4G expression, and overexpression of HNF4G significantly increased the resistance to cisplatin in A549 and HCC827 cells, whereas knockdown of HNF4G had the opposite effect. HNF4G overexpression increased MAPK6 expression and activated the MAPK6/Akt pathway, while an Akt inhibitor reduced the effects of HNF4G on cisplatin resistance. HNF4G bound to the MAPK6 promoter region, promoting MAPK6 expression, according to ChIP-qPCR and luciferase assays.

Conclusion
By binding to the MAPK6 promoter region, HNF4G promotes MAPK6 expression and subsequent Akt phosphorylation, resulting in resistance to cisplatin in lung adenocarcinoma.
Effect of Different Dose of Herbicide on Soil Physico-chemical and Biological Properties after Harvest of Wheat
Authors: Meena Monika , Singh Rajesh Kumar , Dhayal Suman , Meena Pooja Kumari 
Published: March 2023

The purpose of the present experiment is to evaluate the efficacy of herbicide to control of weeds in wheat crop. The field experiment was conducted at Agricultural Research Farm, Division of Agronomy, BHU, Varanasi, India. In the experimental field weeds were controlled by pre-emergence application of herbicides viz.,pendimethalin 30% EC @ 600 g ha-1,pendimethalin 30% EC @ 900 g ha-1, pendimethalin 30% EC @ 1200 g ha-1, pendimethalin 30% EC @ 1500 g ha-1,metribuzin 70% WP @ 210 g ha-1 weed free (two hands weeding) and Untreated Control (Weedy check). Effect of treatments on soil physico-chemical properties like soil bulk density, soil p=H, electric conductivity and organic carbon are not significance variation among the treatments. The available nitrogen in soil after harvest of wheat was more under hand weeded treated plots which was statistically comparable with application of pendimethalin 30% EC @ 900 g ha-1 over the rest of the treatments. However, available phosphorus and potassium in non-significant it did not influenced by the treatments.
Factors Affecting Financial Distress in Small and Medium Enterprises (SMEs) in Indonesia: An Empirical Study
Authors: Aurelia Vanny , Leon Farah Margaretha 
Published: March 2023

Aims: The research conducted aims to see the effect of financial ratios in the ability to predict the possibility of a company in experiencing financial distress.
Study Design: The independent variables in this study are leverage, liquidity and profitability. The control variables consist of firm size and age of the company while the dependent variable is financial distress as measured by the Z-score.
Place and Duration of Study: The sample used in this research is a company listed on the 2017-2021 PEFINDO index on Bursa Efek Indonesia.
Methodology: The number of samples used were 65 companies with a total of 325 observations using purposive sampling.
Results: The results showed that leverage had a positive significant effect on financial distress meanwhile profitability and age had a negative significant effect on financial distress.
Conclusion: The company is expected to minimize the use of leverage which used to fund total assets. In addition, the company is expected to be able to increase company's net profit to minimize it’s possibility in experiencing financial distress and companies with a mature age to keep maintaining leverage and profitability in order to avoid the risk of financial distress. Investors are expected to choose companies by looking at low leverage ratios, high profitability, and companies with old ages so that the risk of investment can be minimized. The government is expected to be able to provide capital to companies so that the company's leverage can be lower as well as an increase company’s profitability as a result of increasing company productivity in doing business.
Estimation of Radon Concentration around Public Spaces and Residential Homes with Altitude within Cities of Delta State, Nigeria
Authors: Ilugo N. T. , Avwiri G. O. , Chad-Umoren Y. E. 
Published: March 2023

This study evaluates the radon concentrations in public places and private residences with different altitudes in selected locations in Delta State. These measurements were carried out using a professional radon monitoring instrument (Alpha GUARD PQ2000 PRO) and a geographical positioning system (GPS-Garmin GPS Map 76S). The recorded mean radon concentration varied from 11.70 ± 5.20 Bq/m3 to 23.90 ±16.60 Bq/m3, which is within the WHO acceptable range (100 Bq/m3). The basement had greater radon concentrations than the upper floors in most situations, although there were few exceptions. The average values of the estimated radiation risk parameters, which include equilibrium equivalent radon concentration, the potential alpha energy concentration, radon exhalation rates, and excess lifetime cancer risk due to exposure to radon radiation from their progeny are 4.7 Bq/m3 to 9.5Bq/m3, 1.20 × 10-3mWL to 2.60 × 10-3mWL, 0.04 × 10-3WML/y to 0.09× 10-3 WML/y, 3.7Bq/m2/h to 7.52Bq/m2/h and 2.10 × 10-3 to 3.30 × 10-3 respectively. The calculated radiation risk factors were all found to be within the recommended limits based on the data obtained. The research region is deemed safe and poses no threat to people.
Life Expectancy Gaps Among Black and White Persons and Contributing Causes of Death in 3 Large US Cities, 2018-2019
Authors: Roesch Pamela T. , Saiyed Nazia S. , Laflamme Emily , De Maio Fernando G. , Benjamins Maureen R. 
Published: March 2023

ImportanceUS cities have substantial, but varying, levels of racial mortality inequities, a consequence of structural racism. As committed partners increasingly pledge to eliminate health inequities, local data are required to focus and unify efforts.ObjectiveTo analyze the contributions of 26 cause-of-death categories to Black to White life expectancy gaps within 3 large US cities.Design, Setting, and ParticipantsIn this cross-sectional study, data were extracted from the 2018 and 2019 National Vital Statistics System Multiple Cause of Death Restricted Use data files for deaths by race, ethnicity, sex, age, place of residence, and underlying and contributing causes of death in Baltimore, Maryland; Houston, Texas; and Los Angeles, California. Life expectancy at birth was calculated for non-Hispanic Black and non-Hispanic White populations overall and by sex using abridged life tables with 5-year age intervals. Data analysis was performed from February to May 2022.Main Outcomes and MeasuresUsing the Arriaga method, the proportion of the Black to White life expectancy gap was calculated overall and by sex for each city that was attributable to 26 cause-of-death categories defined using the International Statistical Classification of Diseases and Related Health Problems, Tenth Revision codes for underlying and contributing causes of death.ResultsA total of 66 321 death records from 2018 to 2019 were analyzed, with 29 057 individuals (44%) were identified as Black, 34 745 (52%) as male, and 46 128 (70%) as aged 65 years and older. Black to White life expectancy gaps were 7.60 years for Baltimore, 8.06 years for Houston, and 9.57 years for Los Angeles. Circulatory diseases, cancer, injuries, and diabetes and endocrine disorders were top contributors to the gaps, although the order and magnitude varied by city. The contribution of circulatory diseases was 11.3 percentage points higher in Los Angeles than in Baltimore (3.76 years [39.3%] vs 2.12 years [28.0%]). The contribution of injuries to Baltimore’s racial gap (2.22 years [29.3%]) was twice as large as in Houston (1.11 years [13.8%]) and Los Angeles (1.36 years [14.2%]).Conclusions and RelevanceBy assessing the composition of Black to White life expectancy gaps for 3 large US cities and categorizing deaths at a more granular level than past studies, this study provides insight into the differing underpinnings of urban inequities. This type of local data can support local resource allocation that more effectively addresses racial inequities.
Near‐Infrared Spectroscopy and Chemometrics in Food and Agriculture
Authors: Pérez‐Marín Dolores C. , Garrido‐Varo Ana 
Published: March 2023

Abstract
Nowadays, the analytical needs of the agrifood industries are not only to comply with regulations to meet quality standards and protect their products from food fraud but also to self‐monitor their processes to improve their efficiency. In addition, a key aspect is to be able to control and trace products along the entire food chain – ‘from farm to fork’ – and for these purposes, near‐infrared spectroscopy (NIRS) sensors are well suited. This article addresses the potential of NIRS to respond to specific issues in agriculture and food products and the main data processing methods used for application development, presenting some case studies related to animal feeds, rendering products, and fruits and vegetables. Moreover, the article focuses on showing the transition between the traditional use of NIRS at laboratory level and the current trend of using NIRS in situ, encouraged by the evolution of instrumentation, with portable or on‐line devices now possible and combining the spectral signal with other information and communication technologies to build decision support systems to assist and support industry. The current and future trends in the use of NIRS combined with chemometric for food products and processed assessment are also reported.
Thiohalobacteraceae fam. nov.
Authors: Sorokin Dimitry Y. , Merkel Alexander Y. 
Published: March 2023

Abstract

Thi.o.ha.lo.bac.ter.a.ce'ae
N.L. masc. n.
Thiohalobacter
, the type genus of the family, ‐
aceae
ending to denote a family; N.L. fem. pl. n.
Thiohalobacteraceae
the
Thiohalobacter
family.

Proteobacteria / Gammaproteobacteria / Thiohalobacterales / Thiohalobacteraceae fam. nov.

The family
Thiohalobacteraceae
consists of aerobic, chemolithoautotrophic, sulfur‐oxidizing bacteria from hypersaline salt lakes. They are moderately halophilic aerobic, bacteria utilizing reduced sulfur compounds as the energy source and assimilating CO
2
via the Calvin–Benson–Bassham cycle. The family is a member of the order
Thiohalobacterales
within the
Gammaproteobacteria
and consists of a single genus
Thiohalobacter
. The family‐level status was established by phylogenomic analysis based on the Genome Taxonomy DataBase classification.

DNA G
+
C content (%)
: 64.2 (genome sequence).

Type genus
:
Thiohalobacter
Sorokin et al. 2010
VP
.
Thiohalophilaceae fam. nov.
Authors: Sorokin Dimitry Y. , Merkel Alexander Y. 
Published: March 2023

Abstract

Thi.o.ha.lo.phi.la.ce'ae N.L. masc. n.
Thiohalophilus
, the type genus of the family, ‐
aceae
ending to denote a family; N.L. fem. pl. n.
Thiohalophilaceae
the
Thiohalophilus
family.

Proteobacteria / Gammaproteobacteria / Thiohalomonadales / Thiohalophilaceae fam. nov.

The family
Thiohalophilaceae
incorporates aerobic, chemolithoautotrophic, sulfur‐oxidizing bacteria from hypersaline habitats. They are moderate halophiles utilizing reduced sulfur compounds as the energy source with either O
2
or nitrite as the electron acceptors. The family is a member of the order
Thiohalomonadales
in the
Gammaproteobacteria
and consists of a single genus
Thiohalophilus
. The family‐level classification in the order
Thiohalomonadales
was established by phylogenomic analysis according to the Genome Taxonomy DataBase method.

DNA G
+
C content
: 57.4 (genome of the type strain).

Type genus
:
Thiohalophilus
Sorokin et al. 2007, VL117.
Humibacter
Authors: Carro Lorena 
Published: March 2023

Abstract

Hu.mi.bac'ter. L. masc. n.
humus
, earth, soil and, in earth sciences or agriculture, humus; N.L. masc. n.
bacter
, rod; N.L. masc. n.
Humibacter
, rod living in the humus.

Actinobacteriota / Actinomycetia / Actinomycetales / Microbacteriaceae / Humibacter

Cells are Gram‐stain‐positive rods (0.3–0.7 × 0.8–1.8 μm), non‐spore‐forming, motile, or nonmotile. Mesophilic and strictly aerobic chemo‐organotroph. Strains have been isolated from different types of soils, wood chips, and sewage sludge compost samples. In addition, one strain was obtained from a small intestine of castrated beef cattle. Produce acid phosphatase, leucine arylamidase, α‐glucosidase, and β‐glucosidase enzymes but not α‐chymotrypsin. The peptidoglycan contains 2,4‐diaminobutyric acid as diagnostic diamino acid and several strains also include ornithine. The major quinones are the menaquinones MK‐11 and MK‐12; in addition, MK‐10 can be present. The characteristic polar lipid is phosphatidylglycerol, while diphosphatidylglycerol and several unidentified phospholipids, glycolipids, aminolipids, and lipids could be present. Phylogenetically, a member of the family
Microbacteriaceae
.

DNA G + C content (mol%)
: 62–68 (HPLC, WGS).

Type species
:
Humibacter albus
Vaz‐Moreira et al. 2008a
VP
.
Tenacibaculum
Authors: Duchaud Eric , Bernardet Jean‐François 
Published: March 2023

Abstract

Te.na.ci.ba'cu.lum. L. adj.
tenax ‐acis
, holding fast, tenacious; L. neut. n.
baculum
, stick; N.L. neut. n.
Tenacibaculum
, rod‐shaped bacterium that adheres to the surface of marine organisms.

Bacteroidetes / Flavobacteriia / Flavobacteriales / Flavobacteriaceae / Tenacibaculum

Members of the genus
Tenacibaculum
are exclusively found in marine environments. Most of them are attached to or associated with the surface of marine organisms such as fish, macroalgae, sponges, and invertebrates. However, some strains were isolated from seawater or marine sediments. Several species are important fish pathogens while others are able to degrade complex polysaccharides such as carrageenans or alginate.

Rods are 0.1–0.7 μm in diameter and 0.4–40 μm in length. Filamentous cells >100 μm long and degenerative spherical cells may occur in older cultures.
Ring‐shaped cells, spores, and gas vesicles are not formed. Cells are nonflagellated. Cells of most, if not all, species are motile by gliding. Gram‐stain‐negative. Cells produce a yellow pigment that is mainly zeaxanthin. Flexirubin‐type pigments are absent. Chemo‐organotrophic and heterotrophic. Aerobic. Catalase‐ and oxidase‐positive. Grow well on media containing seawater. The major respiratory quinone is menaquinone‐6.

DNA G + C content (mol%) from the draft or complete genome sequence analysis
: 29.6–32.2 (38.4 for
Tenacibaculum litopenaei
).

Type species
:
Tenacibaculum maritimum
Suzuki et al. 2001
VP
(basonyms: “
Flexibacter marinus
” Hikida et al. 1979,
Flexibacter maritimus
Wakabayashi et al. 1986
VP
, “
Cytophaga marina
” Reichenbach 1989).
Monoglobus
Authors: Kim Caroline C. , Rosendale Douglas I. , Kelly William J. 
Published: March 2023

Abstract

Mo.no.glo'bus. Gr. masc. adj.
monos
, single, solitary; L. masc. n.
globus
, a ball, sphere; N.L. masc. n.
Monoglobus
single sphere.

Firmicutes / Clostridia / Clostridiales / Ruminococcaceae / Monoglobus

Coccus‐shaped cells, 0.6 μm in diameter. Nonmotile. Endospores are not observed. Gram‐positive‐stain. Colonies are small. Mesophilic. Strictly anaerobic. Cytochrome oxidase‐negative. Catalase‐positive. Grow on
d
‐galacturonic acid,
d
‐xylose,
l‐
arabinose,
d
‐fructose, and pectins from various sources. Major fermentation products include H
2
, CO
2
, acetate, formate, and lactate. Major cellular fatty acids include C
18:1
ω9
c
, an 18C‐straight monounsaturated fatty acid. 16S rRNA gene sequence affiliates the genus
Monoglobus
to the family
Ruminococcaceae
. Isolated from the feces of a healthy human donor residing in New Zealand.

DNA G +C content (mol%)
: 37.2 (genome).

Type species
:
Monoglobus pectinilyticus
Kim et al. 2017
VP
.
Zestosphaera
Authors: St. John Emily 
Published: March 2023

Abstract

Zes.to.sphae'ra. Gr. masc. adj.
zestos
hot, boiling; Gr. fem. n.
sphaera
a ball, a sphere; N.L. fem. n.
Zestosphaera
a hot sphere.

Crenarchaeota / Thermoprotei / Desulfurococcales / Desulfurococcaceae / Zestosphaera

The genus
Zestosphaera
(family
Desulfurococcaceae
) consists of a single species,
Zestosphaera tikiterensis
NZ3
T
. Cells are nonmotile cocci approximately 1.0 μm in diameter.
Zestosphaera
is anaerobic, neutrophilic (pH optimum 6.0) hyperthermophiles with an optimum growth temperature of 82–83°C. Growth has been observed on 0–0.5% NaCl (w/v). Thiosulfate is required for growth. Protein‐rich carbon sources support growth, including yeast extract, casamino acids, gelatin, and tryptone, and growth has not been observed on sugars, carbohydrates, alcohols, or fatty acids. Lipids consist of glycerol dialkyl glycerol tetraethers. An S‐layer is inferred from the genome.
Z. tikiterensis
cells also can form a close association with the symbiotic nanoarchaeote
Candidatus
Nanoclepta minutus, in which
Z. tikiterensis
functions as the host organism. The type species was isolated from a terrestrial geothermal spring in Tikitere, New Zealand.

DNA G + C content (mol%)
:
41.9 (genome analysis).

Type species
:
Zestosphaera tikiterensis
St. John et al. 2019, VL197.
Methylohalomonadales ord. nov.
Authors: Sorokin Dimitry Y. , Merkel Alexander Y. , Whitman William 
Published: March 2023

Abstract

Me.thy.lo.ha.lo.mo.nad.al'es N.L. fem. n.
Methylohalomonas
, the type genus of the order; L. fem. pl. n. suff.‐
ales,
ending to denote an order; N.L. fem. pl. n.
Methylohalomonadales
, order of the genus the
Methylohalomonas
.

Proteobacteria / Gammaproteobacteria / Methylohalomonadales ord. nov.

The order
Methylohalomonadales
includes obligately methylotrophic bacteria from hypersaline salt lakes. They are moderately halophilic aerobes utilizing C
1
compounds as energy and carbon sources via the serine cycle. The order forms a deep‐branching phylogenetic lineage in the
Gammaproteobacteria
and consists of a single family
Methylohalomonadaceae
and genus
Methylohalomonas
. The order‐level classification was established by phylogenomic analysis based on the Genome Taxonomy DataBase classification.

Type genus
:
Methylohalomonas
Sorokin et al. 2007
VP
.
Thiohalobacterales ord. nov.
Authors: Sorokin Dimitry Y. , Merkel Alexander Y. 
Published: March 2023

Abstract

Thi.o.ha.lo.bac.ter.al'es
N.L. masc. n.
Thiohalobacter
, the type genus of the order; L. fem. pl. n. suff. ‐
ales
, ending to denote an order; N.L. fem. pl. n.
Thiohalobacterales
, the order of the genus
Thiohalobacter
.

Proteobacteria / Gammaproteobacteria / Thiohalobacterales ord. nov.

The order
Thiohalobacterales
accommodates obligately chemolithoautotrophic aerobic bacteria utilizing inorganic sulfur compounds as the energy source and assimilating CO
2
via the Calvin–Benson–Bassham cycle. The order is a deeply branching member of the
Gammaproteobacteria
and consists of two families
Thiohalobacteraceae
and
Thiogranaceae,
both of which include one genus. The order‐level status was established by phylogenomic analysis based on the Genome Taxonomy DataBase classification.

Type genus
:
Thiohalobacter
Sorokin et al. 2010
VP
.
Elusimicrobiota
Authors: Brune Andreas , Mies Undine S. 
Published: March 2023

Abstract

E.lu.si.mi.cro.bi.o'ta. N.L. neut. n.
Elusimicrobium
, type genus of the phylum; N.L. neut. pl. n. suff. ‐
ota
, ending to denote a phylum; N.L. neut. pl. n.
Elusimicrobiota
, the
Elusimicrobium
phylum.

Elusimicrobiota
belongs to the PVC superphylum and comprises at least four class‐level lineages of mostly uncultured bacteria. Only the classes
Elusimicrobia
and
Endomicrobia
each have a single cultured representative. Both isolates are strictly anaerobic ultramicrobacteria with a fermentative metabolism and an unusual cell cycle. Like their uncultured relatives in the families
Elusimicrobiaceae
and
Endomicrobiaceae
, they colonize the intestinal tracts of invertebrate and vertebrate hosts and show considerable genome reduction. All other lineages seem to consist of free‐living, strictly anaerobic bacteria that occur in a wide range of aquatic and terrestrial habitats. Members of several apical lineages possess the capacity for aerobic respiration.

Type genus
:
Elusimicrobium
Geissinger et al. 2009, VL132.

The phylum
Elusimicrobiota
contains the classes
Elusimicrobia
Geissinger et al. 2009, VL132 and
Endomicrobia
Zheng et al. 2016, VL180.
Highly Conductive MXene/PEDOT:PSS‐Integrated Poly(N‐Isopropylacrylamide) Hydrogels for Bioinspired Somatosensory Soft Actuators
Authors: Xue Pan , Valenzuela Cristian , Ma Shaoshuai , Zhang Xuan , Ma Jiazhe , Chen Yuanhao , Xu Xinhua , Wang Ling 
Published: March 2023

AbstractSophisticated sensing and actuation capabilities of many living organisms in nature have inspired scientists to develop biomimetic somatosensory soft robots. Herein, the design and fabrication of homogeneous and highly conductive hydrogels for bioinspired somatosensory soft actuators are reported. The conductive hydrogels are synthesized by in situ copolymerization of conductive surface‐functionalized MXene/Poly(3,4‐ethylenedioxythiophene)/poly(styrenesulfonate) ink with thermoresponsive poly(N‐isopropylacrylamide) hydrogels. The resulting hydrogels are found to exhibit high conductivity (11.76 S m−1), strain sensitivity (GF of 9.93), broad working strain range (≈560% strain), and high stability after over 300 loading–unloading cycles at 100% strain. Importantly, shape‐programmable somatosensory hydrogel actuators with rapid response, light‐driven remote control, and self‐sensing capability are developed by chemically integrating the conductive hydrogels with a structurally colored polymer. As the proof‐of‐concept illustration, structurally colored hydrogel actuators are applied for devising light‐driven programmable shape‐morphing of an artificial octopus, an artificial fish, and a soft gripper that can simultaneously monitor their own motions via real‐time resistance variation. This work is expected to offer new insights into the design of advanced somatosensory materials with self‐sensing and actuation capabilities, and pave an avenue for the development of soft‐matter‐based self‐regulatory intelligence via built‐in feedback control that is of paramount significance for intelligent soft robotics and automated machines.