bluuebunny/crossref_metadata_2025_split huggingface dataset:
VdPAT1 encoding a pantothenate transporter protein is required for fungal growth, mycelial penetration and pathogenicity of Verticillium dahliae
Authors: Kamau Stephen Mwangi , Li Yongtai , Sun Tiange , Liu Feng , Zhu Qian-Hao , Zhang Xinyu , Sun Jie , Li Yanjun 
Published: January 2025

IntroductionThe soil-borne vascular fungus Verticillium dahliae is a phytopathogenic fungus known to attack cotton crop causing Verticillium wilt. In previous study, we identified a pantothenate transporter gene (VdPAT1) in V. dahliae which can be induced by root exudates from a susceptible cotton variety.MethodsIn this study, we generated VdPAT1 deletion mutants and complementary strain via homologous recombination by a PEG-mediated transformation method and used for the gene functional characterization.Results and discussionThe VdPAT1 deletion mutants displayed reduced colony growth, melanin production, spore yield and germination rate, showed abnormal mycelial branching and decreased ability of mycelial penetration and utilization of nutrients (carbon, amino acids and vitamin), leading to a lower pathogenicity. Comparative transcriptome analysis of wild-type and mutant strain cultivated on sterilized carboxymethyl cellophane membranes found that the amino sugar and nucleotide sugar metabolism pathway, which was related to chitin synthesis and degradation as well as UDP-glucose synthesis, was the most significantly down-regulated pathway in VdPAT1 deletion mutant. Chitin and β-1,3-glucan content determination found that the chitin content in VdPAT1 deletion mutants was significantly lower, while β-1,3-glucan content was higher than that of wild-type and complementary strains. The ratio change of chitin and β-1,3-glucan content in VdPAT1 deletion mutants might lead to abnormal branching of mycelium, resulting in the reduced penetration ability of V. dahliae. The decreased chitin content in VdPAT1 mutants impaired the fungal cell wall integrity, leading to their increased sensitivity to external stresses.ConclusionTogether, the results demonstrated that VdPAT1 is required for growth, development, resistance to external stresses, mycelial penetration and pathogenicity of V. dahliae.
Gait physiotherapy with motor imagery in people with Parkinson’s disease: a protocol for randomized control GAITimagery trial
Authors: San Martín Valenzuela Constanza , Ramírez Murcia Esperanza , Aznar-Requena Estela , García Sotolongo Dalia , Rosas-Martín Rebeca , Sánchez-Sánchez M. Luz 
Published: January 2025

IntroductionAccording to people with Parkinson’s disease (PD), gait impairments are the most disabling motor symptoms of PD. Recently, motor imagery (MI) has gained notoriety as a gait training technique due to the flexibility of its use, however, it has not been demonstrated that causes a superior effect when included in physiotherapy. This study aims to determine if gait training combined with MI has a greater effect on the gait of people with PD than just gait training.MethodsThe GAITimagery is designed as a double-blind, randomized control trial, including a convenience sample in 2 parallel groups (1:1) with two interventions of 2 sessions per week during 6-week and 8-week follow-up. The initial recruitment will be 88 participants with idiopathic PD and unimpaired cognition state, who will be randomly divided into two groups: GAITimagery (GiG) or the active control Gait group (GaG). Both will perform the same gait exercises but only GiG will include MI training. Gait speed is the primary outcome, while Maximum gait speed (m/s) and Gait speed variability are the secondary results. The tertiary outcomes are related to Quality of life, Daily life activities, Freezing of gait, Balance, Mobility, and Gait performance measures to psychometrics and biomechanics instruments. All results will be measured at baseline (t0), post-training (t1), and follow-up assessment (t2) 8 weeks after finished physiotherapy programs.DiscussionThe GAITimagery program standardizes the application of MI exercises related to the improvement of parkinsonian gait at the same time that monitoring the vividness referred by the participants session by session. The effectiveness of this MI-exclusive program includes subjective and objective measurement tools to detect minimal changes after training. This still-to-be-finish study will support the therapeutic decisions on whether or not to allocate session time to imagery exercises depending on the effect size achieved and the comparison with a control gait training.
Serum albumin-carcinoembryonic antigen ratio as an effective clinical tool for predicting recurrence and overall survival in patients with rectal cancer
Authors: Xie Hailun , Wei Lishuang , Tang Shuangyi , Gan Jialiang 
Published: January 2025

BackgroundThe albumin–carcinoembryonic antigen ratio (ACR), leveraging the strengths of albumin and CEA, has emerged as a promising serum prognostic marker. However, no studies to date have explored the association between ACR and the prognosis of patients with rectal cancer. This study aimed to determine the value of albumin–carcinoembryonic antigen ratio (ACR) in predicting the progression-free survival (PFS) and overall survival (OS) of patients with rectal cancer.MethodsSurvival analysis was conducted using the Kaplan–Meier method, and hazard ratios (HR) were calculated using Cox regression analyses. Nomograms were created based on variables with p &lt; 0.05 in the multivariate Cox regression analysis. The predictive ability of the model was evaluated using the C-index and calibration curve, and its prognostic predictive abilities were compared to those of traditional Tumor Node Metastasis (TNM) stage using discriminant indices.ResultsA total of 736 patients with rectal cancer were included in the study. ACR was significantly higher in patients with poor survival or cancer recurrence. A low ACR was associated with increased tumor invasiveness, longer hospital stays, and higher hospitalization costs. Patients with a high ACR had significantly better PFS (62.9% vs. 35.2%, p &lt; 0.001) and OS (67.0% vs. 37.2%, p &lt; 0.001) than those with a low ACR. ACR can serve as an effective auxiliary tool for pathological staging, especially in patients with stage III–IV disease. The relationship between ACR and mortality risk was L-shaped. ACR is an independent prognostic factor for PFS [HR = 0.581, 95% confidence interval (CI): 0.458–0.738, p &lt; 0.001] and OS (HR = 0.560, 95% CI: 0.435–0.720, p &lt; 0.001) in rectal cancer patients. ACR-based nomograms have good predictive accuracy and outperform traditional TNM stage in predicting prognosis.ConclusionAlbumin–carcinoembryonic antigen ratio is a simple and effective clinical tool for predicting the recurrence and survival of patients with rectal cancer and is a useful supplement to the TNM stage.
Comprehensive evaluation of compound Kushen injection combined with zoledronic acid in treating bone metastasis cancer pain based on meta-analysis and decision tree model
Authors: Zhao Xi , Meng Tianwei , Wang Kaiqiang , Yan Xi , Liu Yuqiang , Li Xinghua 
Published: January 2025

ObjectiveTo evaluate the safety, efficacy, and cost-effectiveness of combining Compound Kushen Injection (CKI) with zoledronic acid in the treatment of bone metastasis-induced cancer pain in malignant tumors.MethodsA comprehensive search of Chinese and English databases identified randomized controlled trials (RCTs) investigating CKI combined with zoledronic acid for bone metastases in malignancies. Methodological quality assessments were performed on all included studies, and a meta-analysis was conducted using RevMan 5.4.1 software. A cost-effectiveness analysis from the perspective of China's healthcare system employed a decision tree model to evaluate the short-term economic impact of the two treatment regimens. Sensitivity analyses assessed the robustness of the results.ResultsFourteen studies involving 1,269 patients were included in the meta-analysis. The results demonstrated that CKI combined with zoledronic acid was more effective than zoledronic acid alone in treating bone metastatic cancer pain (OR = 3.43, 95% CI: 2.51–4.67, P &lt; 0.0001), with no significant difference in adverse reactions between the two groups. Incremental cost-effectiveness ratio (ICER) analysis revealed that the combination therapy incurred an additional cost of ¥18,863.16 for each unit of effect gained compared to zoledronic acid alone. Sensitivity analyses indicated stable results, showing that under the assumption of a willingness-to-pay threshold set at the average per capita disposable income in 2023, the combination of CKI and zoledronic acid was more cost-effective than zoledronic acid alone in treating bone metastatic cancer pain.ConclusionCompared with zoledronic acid alone, the combination of CKI and zoledronic acid offers superior efficacy, high safety, and better cost-effectiveness in the treatment of bone metastasis-induced cancer pain in malignant tumors.
Biomarkers predicting postoperative adverse outcomes in children with congenital heart disease: a systematic review and meta-analysis
Authors: Zhou Shifan , Liu Lu , Jin Xiaochuang , Dorikun Daniel , Ma Songfeng 
Published: January 2025

ObjectiveTo statistically analyze biomarkers predicting postoperative outcomes in children with congenital heart disease (CHD).MethodsPubMed, Embase, Cochrane Library, and Web of Science were performed to search up to February 2024. The measured outcomes were biomarkers, mortality, length of hospital stay, complication rates, and infection rates. Adults with CHD were excluded. Standard deviation or odds ratio (OR) with 95% confidence interval (95% CI) were extracted. A random-effects model synthesized SMDs or ORs with 95% CIs. Sensitivity analysis investigated heterogeneity, and Egger's test assessed publication bias.ResultsSeventeen eligible articles were included, the biomarkers involved include serum lactate, NT-Pro BNP, PaO2, serum creatinine, C1-INH activity, ST2, serum chloride concentration, GH, glycemia, cTOI, NLR, serum albumin, and glucose levels, with 2,888 patients who underwent surgery(modified Norwood procedure, arterial switch procedure, biventricular repair etc.). Serum lactate was higher in the postoperative death group (SMD: 1.18, 95% CI: 0.59–1.77). Lower postoperative N-terminal pro-B-type natriuretic peptide (NT-pro BNP) levels were associated with lower mortality (OR: 0.23, 95% CI: 0.08–0.68) and shorter mechanical ventilation time (OR: 0.40, 95% CI: 0.18–0.90). Higher serum albumin levels were associated with longer hospital stays (OR: 3.12, 95% CI: 1.66–5.84). Significant heterogeneity was found in serum creatinine, B-type natriuretic peptide (BNP), serum lactate, and NT-Pro BNP. Publication bias was detected in some studies.ConclusionSerum lactate, NT-Pro BNP, and serum albumin are reliable biomarkers for predicting adverse outcomes in children with CHD after surgery.Systematic Review RegistrationPROSPERO [CRD42024512753].
Efficacy and safety of tripterygium glycosides combined with ACEI/ARB on diabetic nephropathy: a meta-analysis
Authors: Yao Zhuan’E. , Wang Pengbo , Fu Qinjuan , Song Qiong , Xu Haojian , Zhang Peng 
Published: January 2025

AimsThis study aims to evaluate the efficacy and safety of tripterygium glycosides combined with angiotensin-converting enzyme inhibitors/angiotensin receptor blockers (ACEI/ARBs) in treating Diabetic nephropathy and provide high-level evidence to support its standardized application.MethodsLiteratures were retrieved from PubMed, Web of Science, EMBASE, Cochrane Library, CNKI, Wanfang and VIP databases, the search time frame was defined as from the time of establishment to April 2023. This study only included randomized controlled trials of tripterygium glycosides combined with ACEI/ARB in the treatment of diabetic nephropathy, and the final included studies were identified according to the inclusion and exclusion criteria, and meta-analysis of data was performed using RevMan 5.3 software.ResultsA total of 44 RCTs with 3537 DN patients were included in the study. Compared with the control group, tripterygium glycosides combined with ACEI/ARB significantly reducing 24 h-UTP (24 h urine total protein) [SMD = −1.46, 95% CI (−1.70, −1.23), P &lt; 0.00001], increasing effective rate [RR = 1.23, 95% CI (1.17,1.29), P &lt; 0.00001], elevating serum albumin [SMD = 0.85, 95% CI (0.69, 1.02), P &lt; 0.00001], improving serum creatinine [SMD = −0.35, 95% CI (−0.59, −0.11), P = 0.004], with no difference in BUN (blood urea nitrogen) [SMD = −0.17, 95% CI (−0.48,0.13), P = 0.27], the adverse reactions rate was higher than those of the control group [RR = 1.96, 95%CI (1.43, 2.68), P &lt; 0.0001].ConclusionThis study showed that the combination of tripterygium glycosides and ACEI/ARB was more effective than ACEI/ARB alone. However, the side effects of the combined treatment group were higher than those of the control group, especially liver function damage, which also suggested that its safety in the treatment of diabetic nephropathy was worth considering. Therefore, although tripterygium glycosides provided a choice for the clinical treatment of diabetic nephropathy, its side effects limited its clinical application. In future studies, we need to further optimize tripterygium glycosides and reduce its side effects to ensure the safety of clinical application.
Perception of traumatic childbirth of women, influencing factors and its relationship with post-traumatic stress disorder
Authors: Wei Weiwei , Feng Xiaoyan , Qin Hong , Yang Xiaochang 
Published: January 2025

ObjectivesSome of individuals with psychological birth trauma (PBT) develop into postpartum post-traumatic stress disorder (PP-PTSD) further. Study investigated the prevalence and influencing factors of PBT and its correlation with PP-PTSD, to fill the literature gap in the prevention of postpartum related psychological birth trauma.MethodsA total of 306 women who gave birth vaginally from Chongqing, China participated in this study. Pregnant women filled the basic information when they entered the delivery room and waited for delivery. The psychological birth trauma and posttraumatic stress scales were completed during 1–3 days postpartum. Information on labor and delivery outcomes is available in the hospital's electronic medical record. Variation analysis and Pearson correlation were used on the influencing factors of PBT and the correlation with PP-PTSD.ResultsThe median PBT score of the subjects in this study was 41 points; the incidence rate of PTSD (scores greater than 38) is 5%, with an average score of (22.38 ± 7.126). All dimensions of PBT positively correlated with post-traumatic stress disorder, respectively. Lower score of perceived PBT is associated with work, exercise and learning about delivery; is also associated with less vaginal examinations, the use of pain relief and doula accompaniment.ConclusionThis study suggests that every dimension of PBT should be taken seriously to prevent PP-PTSD. Work, exercise and learning about delivery during pregnancy may be promising protective factors for perceived PBT; the use of pain relief or doula accompaniment are still effective ways to Improve the delivery experience.
Characteristics and risk factors for infection and mortality caused by Klebsiella pneumoniae in patients with acute pancreatitis
Authors: Chen Jing , Xiang Qian , Wu Jia-yu , Zheng Xiao-Jia , Jiang Xiao-yan 
Published: January 2025

BackgroundThe purpose of this study is to investigate the risk factors for carbapenem-resistant Klebsiella pneumoniae (CRKP) infection and death of K. pneumoniae infections in acute pancreatitis (AP) patients.MethodsThis was a retrospective analysis of AP patients with K. pneumoniae infection between January 2019 and April 2024. Logistic regression model was used to determine the risk factors for acquisition of CRKP. Cox proportional hazards regression model was used to identify risk factors for mortality of K. pneumoniae infection.ResultsA total of 113 AP patients experienced K. pneumoniae infections, including 66 with CRKP infection and 47 with CSKP infection. The mortality rate of these patients was 36.3%, with a significantly higher mortality rate in the CRKP group than in the CSKP group (P &lt; 0.001). Only mechanical ventilation (OR = 2.301; 95% CI 1.02-5.20; P = 0.045) was the independent risk factor for CRKP infection in patients with AP. Surgery (HR 0.38; 95% CI 0.20–0.75; P = 0.005) and mechanical ventilation (HR 2.93; 95% CI 1.10–7.82; P = 0.032) were the independent risk factors associated with mortality of K. pneumoniae infection in patients with AP.ConclusionKlebsiella pneumoniae infection has become a threat to patients with AP. Therefore, implementing preventive and control measures based on risk factors is crucial for the prognosis.
PEMETAAN KARAKTERISTIK ARUS LALU LINTAS DENGAN METODE GREENBERG PADA RUAS JALAN KOTA MALANG
Authors: Aldiansyah Fauzi Aldiansyah , Wibisana Hendrata 
Published: January 2025

Volume ruas Jalan Jaksa Agung Suprapto cukup tinggi dan akan terus bertambah seiring berkembangnya jumlah penduduk. Maka dari itu perlu dilakukan analisis karakteristik arus lalu lintas. Pada penelitian ini pendekatan model matematis menggunakan metode Greenberg. Pada ruas Jalan Jaksa Agung Suprapto didapatkan hasil penelitian dan analisis menggunakan metode Greenberg dengan nilai R2 terbesarnya yaitu pada hari Jumâ€™at, tepatnya pada segmen 7 sebesar 0,89. Adapun nilai variabel yang didapatkan dari segmen ini yaitu, Volume Maksimum (Vm) sebesar 5523,05 smp/jam, Kecepatan Bebas (Uf) sebesar 73,01 km/jam, Kecepatan Maksimum (Um) sebesar 9,98 km/jam, dan Kepadatan Jenuh (Dj) sebesar 1504,53 smp/km. Pengaplikasian Sistem Informasi Geografis dalam penelitian ini dilakukan dengan menggunakan hasil penelitian sebagai data atribut dalam peta tematik karakteristik lalu lintas.
REDESAIN STRUKTUR BAWAH BANGUNAN GEDUNG: STUDI KASUS PERENCANAAN PONDASI TAPAK GEDUNG SEKOLAH DASAR NEGERI (SDN) 12 MUNTEI, MENTAWAI
Authors: Fitria Winda , Misriani Merley , Roza Fielda 
Published: January 2025

Kepulauan Mentawai termasuk salah satu daerah yang memanfaatkan sektor pariwisata untuk mendukung perkembangannya. Mentawai merupakan salah satu destinasi favorit wisatawan surfing dari mancanegara. Secara umum wisata di Kepulauan Mentawai memberikan dampak baik terhadap perekonomian daerah setempat. Pembangunan infrastruktur di Kepulauan Mentawai masih tertinggal dibandingkan kota lainnya di daerah Sumatera Barat. Semestinya proses pembangunan di Mentawai perlu mendapat perhatian serius mengingat Mentawai merupakan kawasan yang rawan bencana gempa dan tsunami. Peningkatan potensi gempa dan tsunami tidak diimbangi dengan kesiapan masyarakat dalam menghadapinya. Untuk mengurangi resiko korban jiwa pada daerah rawan gempa standar bangunan tahan gempa sangat penting untuk diterapkan. Bangunan tahan gempa harus diaplikasikan sejak tahapan perencanaan bukan hanya selama proses konstruksi saja. SDN 12 Muntei merupakan salah satu bangunan di Mentawai yang dapat digunakan untuk umum sebagai sekolah dasar. Pemeriksaan terhadap desain awal pondasi bangunan pada penelitian sebelumnya ditemukan bahwa kapasitas dukung izin pondasi tidak mampu memikul beban yang bekerja pada bangunan tersebut, sehingga perlu dilakukan desain ulang penampang pondasi. Setelah dilakukan desain ulang pondasi dengan jenis pondasi tetap pondasi tapak, direkomendasikan pondasi berada pada kedalaman (D) = 1.5meter dengan lebar (B)= 1.3 meter. Nilai SF untuk menentukan daya dukung izin (Qa) diambil sebesar 3. Dari hasil perhitungan dengan dimensi penampang yang baru nilai Qa = 378.76 KN dan nilai beban maksimum bangunan yang bekerja (Pmaks) = 339 KN. Hasil analisis ini menunjukan bahwa penampang tersebut memenuhi syarat dan dapat digunakan
The Impact of Tourism Development on Socio-Economic Conditions for Coastal Communities in Lasiana Beach, Kupang City
Authors: Ariawan I Wayan Adi Putra , Sanam Syul Rosli , Jehane Protasius Tiberius , Riwu Oktovianus Udju 
Published: January 2025

Lasiana Beach is one of the tourist attractions that has become a prime destination due to its natural beauty, located in Lasiana Village, Kupang City. The tourism development at Lasiana Beach is supported by various stakeholders involved, such as the government, the local community, and academics who continuously research to promote sustainable tourism at Lasiana Beach. This is evidenced by data from the East Nusa Tenggara Provincial Tourism and Creative Economy Office, which indicates that the number of tourist visits to Lasiana Beach has consistently increased each year. The growth of tourism at this attraction presents opportunities for the surrounding coastal community to capitalize on the ongoing tourism development. The challenges faced in the tourism development at the Lasiana Beach attraction include the lack of studies assessing the extent of the social and economic impacts on the coastal community of Lasiana Beach. Despite various efforts made by the local government to enhance tourism in the area, it remains unclear how far-reaching the resulting impacts have been. This research was conducted at Lasiana Beach in Kupang City using a qualitative descriptive method. The results of this study were analyzed based on indicators of socio-economic impact, such as the increase in community income, public health, population growth, labor absorption, and the development of the economic structure, which is characterized by economic activities arising from projects such as food stalls, restaurants, transportation services, shops, and others.
Taking time: Auditory statistical learning benefits from distributed exposure
Authors: de Waard Jasper , Theeuwes Jan , Bogaerts Louisa 
Published: January 2025

Abstract
In an auditory statistical learning paradigm, listeners learn to partition a continuous stream of syllables by discovering the repeating syllable patterns that constitute the speech stream. Here, we ask whether auditory statistical learning benefits from spaced exposure compared with massed exposure. In a longitudinal online study on Prolific, we exposed 100 participants to the regularities in a spaced way (i.e., with exposure blocks spread out over 3 days) and another 100 in a massed way (i.e., with all exposure blocks lumped together on a single day). In the exposure phase, participants listened to streams composed of pairs while responding to a target syllable. The spaced and massed groups exhibited equal learning during exposure, as indicated by a comparable response-time advantage for predictable target syllables. However, in terms of resulting long-term knowledge, we observed a benefit from spaced exposure. Following a 2-week delay period, we tested participants’ knowledge of the pairs in a forced-choice test. While both groups performed above chance, the spaced group had higher accuracy. Our findings speak to the importance of the timing of exposure to structured input and also for statistical learning outside of the laboratory (e.g., in language development), and imply that current investigations of auditory statistical learning likely underestimate human statistical learning abilities.