bluuebunny/crossref_metadata_2025_split huggingface dataset:
Mitigation of aircraft noise-induced vascular dysfunction and oxidative stress by exercise, fasting, and pharmacological α1AMPK activation: molecular proof of a protective key role of endothelial α1AMPK against environmental noise exposure
Authors: Kvandová Miroslava , Rajlic Sanela , Stamm Paul , Schmal Isabella , Mihaliková Dominika , Kuntic Marin , Bayo Jimenez Maria Teresa , Hahad Omar , Kollárová Marta , Ubbens Henning , Strohm Lea , Frenis Katie , Duerr Georg Daniel , Foretz Marc , Viollet Benoit , Ruan Yue , Jiang Subao , Tang Qi , Kleinert Hartmut , Rapp Steffen , Gericke Adrian , Schulz Eberhard , Oelze Matthias , Keaney John F , Daiber Andreas , Kröller-Schön Swenja , Jansen Thomas , Münzel Thomas 
Published: March 2023

AbstractAimsEnvironmental stressors such as traffic noise represent a global threat, accounting for 1.6 million healthy life years lost annually in Western Europe. Therefore, the noise-associated health side effects must be effectively prevented or mitigated. Non-pharmacological interventions such as physical activity or a balanced healthy diet are effective due to the activation of the adenosine monophosphate-activated protein kinase (α1AMPK). Here, we investigated for the first time in a murine model of aircraft noise-induced vascular dysfunction the potential protective role of α1AMPK activated via exercise, intermittent fasting, and pharmacological treatment.Methods and resultsWild-type (B6.Cg-Tg(Cdh5-cre)7Mlia/J) mice were exposed to aircraft noise [maximum sound pressure level of 85 dB(A), average sound pressure level of 72 dB(A)] for the last 4 days. The α1AMPK was stimulated by different protocols, including 5-aminoimidazole-4-carboxamide riboside application, voluntary exercise, and intermittent fasting. Four days of aircraft noise exposure produced significant endothelial dysfunction in wild-type mice aorta, mesenteric arteries, and retinal arterioles. This was associated with increased vascular oxidative stress and asymmetric dimethylarginine formation. The α1AMPK activation with all three approaches prevented endothelial dysfunction and vascular oxidative stress development, which was supported by RNA sequencing data. Endothelium-specific α1AMPK knockout markedly aggravated noise-induced vascular damage and caused a loss of mitigation effects by exercise or intermittent fasting.ConclusionOur results demonstrate that endothelial-specific α1AMPK activation by pharmacological stimulation, exercise, and intermittent fasting effectively mitigates noise-induced cardiovascular damage. Future population-based studies need to clinically prove the concept of exercise/fasting-mediated mitigation of transportation noise-associated disease.
“It was a very awkward consultation because I didn’t know”—general practice staff experiences and challenges in providing healthcare to gender and sexual minority youth in Aotearoa New Zealand
Authors: Carroll Rona , Morgan Sonya J , Ker Alex , Garrett Susan M , Rose Sally B , Gardiner Tracey , McKinlay Eileen M 
Published: March 2023

Abstract

Background
Rainbow young people (RYP; people of diverse genders, sexualities, and variations in sex characteristics) face barriers accessing primary care, often report negative experiences and the need to educate clinicians on rainbow-specific issues. This study explores general practice staff views and experiences of providing care to RYP.

Methods
Three focus groups were held with staff from 5 general practices in an urban area of Aotearoa New Zealand (25 participants in total). Practices were purposively selected to include some known to be more “rainbow-friendly” seeing larger numbers of RYP and some with no particular focus on RYP. All members of each practice were invited to participate, including administration and reception staff. Focus groups were audio-recorded, transcribed, and analysed in NVivo using inductive thematic analysis.

Results
Four main themes were identified: (i) Practice experience, (ii) Feeling awkward, (iii) Knowledge and training, and (iv) Structural barriers. Differences were apparent in health provider knowledge, comfort, and experience in providing care to RYP. Participants identified a lack of knowledge and training and wanted more resources and education. Systems limitations were common (e.g. with IT systems for recording gender) and often contributed to awkward clinical encounters.

Conclusions
Participants recognized shortcomings in their training, knowledge, and level of confidence providing care to RYP and expressed a desire to improve their competency. Further work is needed to identify and trial practical strategies that help improve communication skills, knowledge, and the delivery of more equitable healthcare to RYP.
A Social Exergame Intervention to Promote Physical Activity, Social Support, and Well-Being in Family Caregivers
Authors: Lin Xin Yao , Zhang Lin , Yoon Saiyeon , Zhang Ruoying , Lachman Margie E 
Published: March 2023

Abstract

Background and Objectives
Family caregivers often experience a high level of stress, social isolation, a sedentary lifestyle, and poor mental and physical health. An exergame intervention was developed to promote physical activity and well-being in family caregivers and to test social support as a mechanism for behavior change.

Research Design and Methods
The current study was a randomized pilot trial (N = 76) to compare the effectiveness of Go&Grow (social vs nonsocial exergame) to promote well-being through increased social support and physical activity for family caregivers over a 6-week intervention.

Results
The treatment group increased significantly more than the control group in well-being (management of distress) and social support (satisfaction with contact quality). Social support served as a mechanism (mediator and moderator): The treatment group increased more than the control group in satisfaction with social contact quality, which led to more positive affect and less loneliness. Moreover, those in the treatment group who increased more in overall social support and knowing others’ experiences increased their steps more than those with less support, whereas the change in steps for the control group was not related to a support level. Those in the treatment group who used more social features of the app had a greater increase in steps compared with those who used it less.

Discussion and Implications
Social support in technology interventions is a promising direction to promote caregivers’ well-being and physical activity. Social support served as a mechanism of behavior change that can inform more engaging, sustainable, portable, and scalable interventions in the future for sedentary and socially isolated family caregivers.
Clinical Trial Registration Number: NCT05032872
Looking at the Data Governance Act and Beyond: How to Better Integrate Data Intermediaries in the Market Order for Data Sharing
Authors: Richter Heiko 
Published: March 2023

Abstract
This article enquires into the current prospects for data intermediaries in the context of competition and innovation policies. It asks what the conditions for and means to fulfil these promises are. This requires looking at the evolving legal framework – including the recently enacted Regulation (EU) 2022/686 ‘Data Governance Act’, which affects the incentives of data intermediaries and market actors. In particular, this article explores the obstacles for the establishment of data intermediaries, the context for their activities and the necessary conditions to be set, and complimentary measures to be taken to make them work. The overall goal is to discuss how the findings would translate into viable policy options to advance the regulatory framework that would contribute to an effective market design for data sharing.**
Critical thinking in musicians’ health education. Findings from four workshops with experts (Part I)
Authors: Matei Raluca , Phillips Keith 
Published: March 2023

SummaryHealth education encompasses building health knowledge, but also training skills such as critical thinking, that guide individuals’ ability to access, understand and use health information to take care of their own health (WHO, 1998). This study aimed to document expert discussions on the content of an ideal health education curriculum for higher music education (HME) students in the UK, integrating critical thinking. Four interdisciplinary workshops were conducted, where 67 experts in relevant fields discussed the content of four lists created based on literature reviews (cognitive biases, logical fallacies, critical appraisal tools and health topics). Notes taken during the discussions were thematically analysed. Most of the participants thought that the topics and tools were relevant. Two of four identified themes are reported in this paper, which represents the first of a two-part series: (1) critical thinking applied to health; and (2) misconceptions. This is the first attempt to document conversations aimed at using the applied knowledge of key stakeholders to discuss the content of an ideal health education curriculum integrating critical thinking, for conservatoire students.
Scaling from eDNA to biomass: controlling allometric relationships improves precision in bycatch estimation
Authors: Urban P , Bekkevold D , Degel H , Hansen B K , Jacobsen M W , Nielsen A , Nielsen E E 
Published: March 2023

AbstractEnvironmental DNA (eDNA) has attracted interest in relation to fisheries, with its possibilities for species identification and promises for species quantification. In the context of fisheries catches, eDNA can be most useful for the estimation of bycatch proportions. The assessment of species mixtures in large catches (&gt;1000 t) is challenging, especially when morphologically similar species are to be differentiated. We used an experimental set-up to simulate industrial sprat fishery catches, and tested two types of water (blood water and discharge water) derived from this simulated fishery for their suitability in reliable species quantification. We analysed nine mixtures of sprat and herring—the main bycatch species. Species-specific quantitative PCR was used for species identification and quantification. Species-to-species weight fractions and eDNA fractions in mixtures showed a strong correlation. Accounting for size-based differences in DNA abundance (allometrically scaled weight) reduced the estimated standard error on weight fraction prediction from 0.064 to 0.054 in blood water, and from 0.080 to 0.075 in discharge water when comparing the weight-based model with the allometrically scaled weight model, respectively. Accounting for allometric scalling in genetic analyses of fisheries process water can serve as a more precise method for the assessment of bycatch, thus in a wider sense improve the quality of fisheries-dependent data.
Blockchain-enabled immutable, distributed, and highly available clinical research activity logging system for federated COVID-19 data analysis from multiple institutions
Authors: Kuo Tsung-Ting , Pham Anh , Edelson Maxim E , Kim Jihoon , Chan Jason , Gupta Yash , Ohno-Machado Lucila ,   The R2D2 Consortium, Anderson David M , Balacha Chandrasekar , Bath Tyler , Baxter Sally L , Becker-Pennrich Andrea , Bell Douglas S , Bernstam Elmer V , Ngan Chau , Day Michele E , Doctor Jason N , DuVall Scott , El-Kareh Robert , Florian Renato , Follett Robert W , Geisler Benjamin P , Ghigi Alessandro , Gottlieb Assaf , Hinske Ludwig C , Hu Zhaoxian , Ir Diana , Jiang Xiaoqian , Kim Katherine K , Kim Jihoon , Knight Tara K , Koola Jejo D , Kuo Tsung-Ting , Lee Nelson , Mansmann Ulrich , Matheny Michael E , Meeker Daniella , Mou Zongyang , Neumann Larissa , Nguyen Nghia H , Nick Anderson , Ohno-Machado Lucila , Park Eunice , Paul Paulina , Pletcher Mark J , Post Kai W , Rieder Clemens , Scherer Clemens , Schilling Lisa M , Soares Andrey , SooHoo Spencer , Soysal Ekin , Steven Covington , Tep Brian , Toy Brian , Wang Baocheng , Wu Zhen R , Xu Hua , Yong Choi , Zheng Kai , Zhou Yujia , Zucker Rachel A 
Published: March 2023

Abstract

Objective
We aimed to develop a distributed, immutable, and highly available cross-cloud blockchain system to facilitate federated data analysis activities among multiple institutions.

Materials and Methods
We preprocessed 9166 COVID-19 Structured Query Language (SQL) code, summary statistics, and user activity logs, from the GitHub repository of the Reliable Response Data Discovery for COVID-19 (R2D2) Consortium. The repository collected local summary statistics from participating institutions and aggregated the global result to a COVID-19-related clinical query, previously posted by clinicians on a website. We developed both on-chain and off-chain components to store/query these activity logs and their associated queries/results on a blockchain for immutability, transparency, and high availability of research communication. We measured run-time efficiency of contract deployment, network transactions, and confirmed the accuracy of recorded logs compared to a centralized baseline solution.

Results
The smart contract deployment took 4.5 s on an average. The time to record an activity log on blockchain was slightly over 2 s, versus 5–9 s for baseline. For querying, each query took on an average less than 0.4 s on blockchain, versus around 2.1 s for baseline.

Discussion
The low deployment, recording, and querying times confirm the feasibility of our cross-cloud, blockchain-based federated data analysis system. We have yet to evaluate the system on a larger network with multiple nodes per cloud, to consider how to accommodate a surge in activities, and to investigate methods to lower querying time as the blockchain grows.

Conclusion
Blockchain technology can be used to support federated data analysis among multiple institutions.
Aorto-bronchial fistula masquerading as haematemesis: a rare late complication of thoracic aortic vascular grafts
Authors: Peacock Timothy , Limmer Alexandra M , Zahid Assad 
Published: March 2023

AbstractAorto-bronchial fistula is an exceedingly rare pathology with high mortality. Late vascular graft infection may occur secondary to haematogenous seeding of bacteria from a distant source such as gastrointestinal infection. We present an unusual case of aorto-bronchial fistula masquerading as haematemesis in a patient with sigmoid diverticulitis, and review the pathophysiology, diagnosis, surgical and endovascular management of aorto-bronchial fistulas.
Insights into the binding of Ag ions with SilE model peptides: an NMR and MS coupled approach
Authors: Zingale Gabriele Antonio , Oliveri Valentina , Grasso Giuseppe 
Published: March 2023

Abstract
The diffuse and renewed use of silver as antimicrobial agent has caused the development of resistance to silver ions in some bacterial strains, posing a serious threat for health systems. In order to cast light on the mechanistic features of resistance, here, we aimed to understand how silver interacts with the periplasmic metal-binding protein SilE which is engaged in bacterial silver detoxification. This aim was addressed by studying two peptide portions of SilE sequence (SP2 and SP3) that contain the putative motifs involved in Ag+ binding. We demonstrate that SP2 model peptide is involved in silver binding through its histidine and methionine residues in the two HXXM binding sites. In particular, the first binding site is supposed to bind the Ag+ ion in a linear fashion, while the second binding site complexes the silver ion in a distorted trigonal planar fashion. We propose a model where the SP2 peptide binds two silver ions when the concentration ratio Ag+/SP2 is ≥10.0. We also suggest that the two binding sites of SP2 have different affinity for silver. This evidence comes from the change in the path direction of the Nuclear Magnetic Resonance (NMR) cross-peaks upon the addition of Ag+. Here, we report the conformational changes of SilE model peptides occurring upon silver binding, monitored at a deep level of molecular details. This was addressed by a multifaceted approach, combining NMR, circular dichroism, and mass spectrometry experiments.
Knockdown of the SELENOK gene induces ferroptosis in cervical cancer cells
Authors: Abdurahman Anwar , Li Yu , Jia Shi-Zheng , Xu Xin-Wen , Lin Shu-Jing , Ouyang Pei , jun He Zhi , Zhang Zhong-Hao , Liu Qiong , Xu Ying , Song Guo-Li 
Published: March 2023

Abstract
Selenoprotein K (SELENOK) is one of the endoplasmic reticulum (ER) proteins that mainly functions in the regulation of ER stress, calcium flux, and antioxidant defense. Reactive oxygen species (ROS) is one of the key indicators of ferroptosis, and SELENOK inhibition could disrupt ROS balance, and consequently might cause ferroptosis. However, there are no previous studies about the mechanism of SELENOK in ferroptosis by regulating ROS. In this study, we report the effect of SELENOK inhibition on cell proliferation, viability, iron recycling–associated proteins, ROS, antioxidant enzymes, and lipid peroxidation of cervical cancer cells (HeLa cells). The results showed that ROS levels and iron-dependent lipid peroxidation were significantly enhanced, whereas cell viability and proliferation were significantly downregulated, and resulted in marked reductions in tumor size after SELENOK knockdown. SELENOK knockdown also caused steep decreases in glutathione peroxidase 4/glutathione levels and deterioration in ROS scavenging ability, and exacerbated ferroptosis in HeLa cells. Our findings elucidated that SELENOK knockdown could shrink tumor size by regulating ferroptosis, which might provide a theoretical basis for treating cervical cancer.
Tubulovascular protection from protease-activated receptor-1 depletion during AKI-to-CKD transition
Authors: Lok Sarah W Y , Yiu Wai Han , Zou Yixin , Xue Rui , Li Hongyu , Ma Jingyuan , Chen Jiaoyi , Chan Loretta Y Y , Lai Kar Neng , Tang Sydney C W 
Published: March 2023

ABSTRACT

Background
Thromboembolic events are prevalent in chronic kidney disease (CKD) patients due to increased thrombin generation leading to a hypercoagulable state. We previously demonstrated that inhibition of protease-activated receptor-1 (PAR-1) by vorapaxar reduces kidney fibrosis.

Methods
We used an animal model of unilateral ischemia–reperfusion injury-induced CKD to explore the tubulovascular crosstalk mechanisms of PAR-1 in acute kidney injury (AKI)-to-CKD transition.

Results
During the early phase of AKI, PAR-1-deficient mice exhibited reduced kidney inflammation, vascular injury, and preserved endothelial integrity and capillary permeability. During the transition phase to CKD, PAR-1 deficiency preserved kidney function and diminished tubulointerstitial fibrosis via downregulated transforming growth factor-β/Smad signaling. Maladaptive repair in the microvasculature after AKI further exacerbated focal hypoxia with capillary rarefaction, which was rescued by stabilization of hypoxia-inducible factor and increased tubular vascular endothelial growth factor A in PAR-1-deficient mice. Chronic inflammation was also prevented with reduced kidney infiltration by both M1- and M2-polarized macrophages. In thrombin-induced human dermal microvascular endothelial cells (HDMECs), PAR-1 mediated vascular injury through activation of NF-κB and ERK MAPK pathways. Gene silencing of PAR-1 exerted microvascular protection via a tubulovascular crosstalk mechanism during hypoxia in HDMECs. Finally, pharmacologic blockade of PAR-1 with vorapaxar improved kidney morphology, promoted vascular regenerative capacity, and reduced inflammation and fibrosis depending on the time of initiation.

Conclusions
Our findings elucidate a detrimental role of PAR-1 in vascular dysfunction and profibrotic responses upon tissue injury during AKI-to-CKD transition and provide an attractive therapeutic strategy for post-injury repair in AKI.
Practice variation in re-resection for recurrent glioblastoma: A nationwide survey among Dutch neuro-oncology specialists
Authors: van Opijnen Mark P , de Vos Filip Y F , Nabuurs Rob J A , Snijders Tom J , Nandoe Tewarie Rishi D S , Taal Walter , Verhoeff Joost J C , van der Hoeven Jacobus J M , Broekman Marike L D 
Published: March 2023

Abstract

Background
Despite current best treatment options, a glioblastoma almost inevitably recurs after primary treatment. However, in the absence of clear evidence, current guidelines on recurrent glioblastoma are not well-defined. Re-resection is one of the possible treatment modalities, though it can be challenging to identify those patients who will benefit. Therefore, treatment decisions are made based on multidisciplinary discussions. This study aimed to investigate the current practice variation between neuro-oncology specialists.

Methods
In this nationwide study among Dutch neuro-oncology specialists, we surveyed possible practice variation. Via an online survey, 4 anonymized recurrent glioblastoma cases were presented to neurosurgeons, neuro-oncologists, medical oncologists, and radiation oncologists in The Netherlands using a standardized questionnaire on whether and why they would recommend a re-resection or not. The results were used to provide a qualitative analysis of the current practice in The Netherlands.

Results
The survey was filled out by 56 respondents, of which 15 (27%) were neurosurgeons, 26 (46%) neuro-oncologists, 2 (4%) medical oncologists, and 13 (23%) radiation oncologists. In 2 of the 4 cases, there appeared to be clinical equipoise. Overall, neurosurgeons tended to recommend re-resection more frequently compared to the other specialists. Neurosurgeons and radiation oncologists showed opposite recommendations in 2 cases.

Conclusions
This study showed that re-resection of recurrent glioblastoma is subject to practice variation both between and within neuro-oncology specialties. In the absence of unambiguous guidelines, we observed a relationship between preferred practice and specialty. Reduction of this practice variation is important; to achieve this, adequate prospective studies are essential.
Chromoblastomycosis Treated With Posaconazole and Adjunctive Imiquimod: Lending Innate Immunity a Helping Hand
Authors: Logan Clare , Singh Manuraj , Fox Natalya , Brown Gordon , Krishna Sreedhar , Gordon Kristiana , Macallan Derek , Bicanic Tihana 
Published: March 2023

AbstractChromoblastomycosis (CBM) is a difficult-to-treat, chronic fungal infection of the skin and subcutaneous tissue. The evidence base for treatment is scarce, with no standardized therapeutic approach. Chronicity of CBM infection is postulated to be due in part to a failure of host cell–mediated immunity to generate a proinflammatory response sufficient for fungal clearance. We present a case of a chronic chromoblastomycosis lesion of the hand present for nearly 4 decades, previously refractory to itraconazole monotherapy, that was successfully treated with a combination of posaconazole and adjunctive immunotherapy with topical imiquimod, a Toll-like receptor 7 agonist. Serial biopsies and images demonstrate the clinical and histopathological improvement of the lesion. Randomized trials of antifungal therapy with adjunctive imiquimod are warranted to determine whether a combination of antifungal and host-directed therapy improves outcomes for this neglected tropical mycosis.