bluuebunny/crossref_metadata_2025_split huggingface dataset:
In vivo Brain Estrogen Receptor Expression By Neuroendocrine Aging And Relationships With Gray Matter Volume, Bio-Energetics,
and Clinical Symptomatology
Authors: Mosconi Lisa , Jett Steven , Nerattini Matilde , Andy Caroline , Yepez Camila Boneu , Zarate Camila , Carlton Caroline , Kodancha Vibha , Schelbaum Eva , Williams Schantel , Pahlajani Silky , Loeb-Zeitlin Susan , Havryliuk Yelena , Andrews Randolph , Pupi Alberto , Ballon Douglas , Kelly James , Osborne Joseph , Nehmeh Sadek , Fink Matthew , Berti Valentina , Matthews Dawn , Dyke Jonathan , Brinton Roberta Diaz 
Published: February 2023

Abstract
17β-estradiol,the most biologically active estrogen, exerts wide-ranging effects in brain through its action on estrogen receptors (ERs), influencing higher-order cognitive function and neurobiological aging. However, our knowledge of ER expression and regulation by neuroendocrine aging in the living human brain is limited. This in vivo multi-modality neuroimaging study of healthy midlife women reveals progressively higher ER density over the menopause transition in estrogen-regulated networks. Effects were independent of age and plasma estradiol levels, and were highly consistent, correctly classifying all women as being post-menopausal or not. Higher ER density was generally associated with lower gray matter volume and blood flow, and with higher mitochondria ATP production, possibly reflecting compensatory mechanisms. Additionally, ER density predicted changes in thermoregulation, mood, cognition, and libido. Our data provide evidence that ER density impacts brainstructure, perfusion and energy production during female endocrine aging, with clinical implications for women’s health.
A new view on the metrology of digital twin objects of production processes on the example of assessing the serviceability of metal cutting tools
Authors: Shvedenko Vladimir , Shchekochikhin Oleg 
Published: February 2023

Abstract
The basic property of the digital twin is its metrology, which includes methods and means of ensuring the uniformity of measurements and achieving their required accuracy. The purpose of this study is to justify the basic functions of digital twin metrology - measuring and correcting an "exact" copy of a physical object, with justification of the frequency of this measurement based on the emerging risks of disturbing the functionality of the technical object. The methodology of using object-process data model when creating digital twins is presented. Methodology for calculating failure probability of technical object depending on probabilistic characteristics of its individual properties is shown. The variants of periodicity of measurements of changing properties of technical object to ensure the adequacy of digital model taking into account the probability of failure occurrence are shown. The variant of estimation of economic efficiency of digital twin of technical object is considered. The scheme for determination of economically justified period of technical object operation taking into account probability of failure occurrence is shown. The article considers an example of creation of digital model of metal-cutting tools and control process of wear of their cutting edges. The presented mathematical model allows virtual prediction of the tool cutting edge wear and probability of failure occurrence. The inverse relations between the physical object and the mathematical model allow to correct the empirical coefficients.
Adsorption and Diffusion Properties of Tobacco Composition and Additive: A Multiscale Theoretical Study
Authors: Xia Qian , Wang Jun , Jiang Jian , Hu Anfu , Wu Jian , Lu Xinbo , Xiao Weiqiang , Zhou Guojun , Zhuang Guilin 
Published: February 2023

Abstract
Unveiling the influence effects of H2O and others (propylene glycol, glycerol and nicotine) on adsorption and diffusion in tobacco is of great importance in cigarette industry while being a challenge issue under three different factors (temperature, concentration and aperture size). Herein, via first-principles density functional theory ( DFT ) simulations, it is found that H2O tends to be adsorbed on O-top sites rather than H-top sites due to the stronger H-bond attraction, on which existed H2O is also easy to desorb because of the weak physical adsorption. Moreover, via molecular dynamics ( MC ) calculations, under 300 K − 500 K range, the diffusion of H2O and other components increases as increasing temperature due to the intensified thermal exercise. Regarding effect of concentration (4% − 14%), with upgrading the concentration, the diffusion property elevates and then decreases for H2O and others, which may be related to the stability of the system caused by H-bonds and the free accommodation space of cellulose structure. Focused on influence of aperture size, as the pore size increases, the diffusion of H2O and others increases significantly due to the expansion of the free space and the diffusivity of H2O is higher than that of others on account of smaller structural form.
Language disorders as comorbidity in enuretic children: prevalence and impact on treatment
Authors: FERRARA Pietro , CAMMISA Ignazio , GALLO Giorgia , Zona Margherita , MASTRICCI Rosanna , MATARRELLI Chiara , SACCO Roberto , VERROTTI Alberto 
Published: February 2023

Abstract
PURPOSE
Several studies have recorded an association between nocturnal enuresis (NE) and developmental delays. Language disorders are one of the main comorbidities in enuretic children, affecting therapy outcome and prognosis.
METHODS
The study was carried out within our pediatric outpatient clinic in Campus Bio-Medico University Hospital Foundation of Rome, enrolling a total of 226 enuretic children, of which 21 with a language disorder. Data concerning NE were collected through questionnaire and interview with parents and a bladder diary. The language disorders was secondary investigated. The chi-squared test was used to evaluate the correlation between therapy outcome and language disorders.
RESULTS
Our sample was composed of 226 enuretic children, with a mean age of 7.67 years. 217 children were subjected to pharmacological treatments, while 9 children, with mild clinical symptoms, did not take any drugs. It was observed that 49% of enuretic children did not respond to therapy or relapsed, while 51% responded in the first cycle. 21/226 (9%) of enuretic children had a language disorder, of which 15 subjected to pharmacological therapy: 12/15 (80%) did not respond to therapy and 3/15 (20%) responded (p = 0.024).
CONCLUSIONS
Language disorders could impact NE therapy response, underlying the influence of comorbidities and the importance of a global assessment in enuretic children.
Allogenic stem cells for anal Crohn’s fistula: treating early improves the deep response rate
Authors: Fathallah Nadia , Siproudhis Laurent , Akaffou Mélissa , Haouari Mohamed Amine , Landemaine Amandine , Pommaret Elise , Spindler Lucas , Brochard Charlène , Bouguen Guillaume , Parades Vincent 
Published: February 2023

Abstract
Purpose:
The aim of this study was to evaluate the real-life clinical and radiological efficacy of darvadstrocel injection into complex perianal fistulas in Crohn’s disease. Secondary endpoints were to assess symptomatic efficacy, outcomes and factors associated with complete combined clinical-radiological response (deep response).
Methods:
After marketing the product in France, all patients treated consecutively were included. A complete clinical response was defined by a complete closure of all external openings with no discharge on pressure. A partial response was defined by closure of ≥ 50% of external openings with no discharge on pressure. A complete radiological response (MRI), evaluated at least after six months of follow-up, was defined by a completely fibrotic sequela without abscess.
Results:
Forty-three patients were included (M/F: 22/21, median age 37 [26-45] years). The fistulas of all patients were already drained with seton(s) and were on biologic treatment. After a median follow-up of 383 [359-505] days, 28 (65%) patients showed a clinical response (22 complete and 6 partial). Only 16 (37%) achieved a deep response. The PDAI decreased significantly after treatment: 39 (91%) patients reported symptomatic improvement in terms of discharge, pain, and induration, and 28 (65%) no longer had any perineal symptoms. Only a short history of Crohn’s disease < 3 years was significantly associated with deep response (OD 4.5 [1.0-19.1], p = 0.04).
Conclusion:
Darvadstrocel injection resulted in a clinical response for two thirds of patients and deep response for one third. A shorter duration of Crohn’s disease was associated with deep response.
Health, wellbeing and nutritional impacts for students and families after two years of government-funded school lunches in low advantage schools in Aotearoa New Zealand.
Authors: McKelvie-Sebileau Pippa , Swinburn Boyd , Glassey Rachael , Leach David Tipene , Gerritsen Sarah 
Published: February 2023

Abstract
Objective: In 2020, a government-funded school lunch program was introduced in a quarter of New Zealand schools selected based on high levels of socio-economic barriers. We report family (whānau), student and principal perspectives on the impact of the first two years of this healthy school lunch program.
Methods: We conducted five focus groups (2 with secondary students and 3 with family members) and four school principal interviews. Thematic analysis was carried out to develop themes describing the health, wellbeing and nutritional impact of the program. Participating schools represent a range of contexts: primary and secondary, and schools using on-site kitchens and cooks or schools choosing to receive meals delivered by external caterers.
Results: Family participants were 82% Indigenous Māori and self-identified as having ‘borderline’ (73.5%) or no financial security (8.8%). Seven positives themes were identified: improved food security, enhanced equity, increased appreciation of healthy foods for students, enhanced mana (wellbeing) for all, reduced financial hardship and stress for families, opportunities for nutritional learning and that appreciation and uptake happen over time. Four negative impact themes were identified: low uptake that created food waste, perception that healthy food is not palatable for students, lack of knowledge of the program and loss of agency for students.
Conclusions: This is the largest intervention in nutrition and food security for children ever to be introduced in New Zealand. The first two years have resulted in important wellbeing and financial benefits for students and families involved, particularly when the school environment promotes uptake. More involvement of students and family members is essential.
Early skin graft necrosis delimitation by postoperative laser speckle analysis
Authors: Pinho André , Brinca Ana , Vieira Ricardo 
Published: February 2023

Abstract
Necrosis complicates up to 60% of skin grafts, but its extension might take several weeks to become fully established. Physical examination has poor accuracy in its early prediction. Laser speckle contrast imaging (LSCI) is a noncontact, in vivo, validated technology for the study of microcirculation of skin grafts. We sought to assess, in humans, if it is possible (with LSCI) to predict on day (D) 14 the extension of skin graft necrosis on D28.
Ten consecutive adult patients who underwent skin malignancy excision on the face (n=3), scalp (n=2), forearm (n=2), pretibial region (n=2) or plantar surface (n=1) and skin graft closure were included. Skin graft perfusion was assessed with LSCI on D14, and hypoperfused regions were highlighted. Clinical pictures of skin grafts on D28 were evaluated, and established necrotic regions were delimited. Then, we assessed whether hypoperfused regions identified on D14 overlapped, in location and size, with those regions of established clinical necrosis on D28.
Graft necrosis extension on D28 ranged from 0.5-21% on the face, 12-48% on the scalp, 0-15% on the forearm, 31-45% on the leg and 15% on the plantar surface. Approximately 89% (17 out of 19) of individual hypoperfused regions identified on D14 were also present on D28. The median extent of necrosis on D28 was 21% of the graft area, while on D14, hypoperfused regions corresponded to 23% (p=0.23).
We demonstrated that it is possible to predict and delimitate necrotic regions of skin grafts in a relatively early stage by LSCI. On D28, they extensively overlapped with the LSCI hypoperfused regions on D14. Therefore, we can identify the subset of patients at high risk of graft necrosis and anticipate the need for prolonged medical and wound care.
Pedigree selection in two Egyptian cotton crosses for some traits
Authors: El- Seidy El-Sayed , Abdel-Hafez Abdel-Aziz , Rashwan Emad El- Deen , Darwesh Ashraf , Abdel-Hafez Aml 
Published: February 2023

One cycle of direct Pedigree selection was performed with selection intensity 5 % to improve productivity with accepTable fiber quality of the two Egyptian cotton ( G. barbadense L.) cross combinations. Comparing mean performance of F2 with those of F3 generation revealed increased mean values for all traits with advanced generations from F2 to F3, indicating accumulation of increasing alleles. The phenotypic variance for all traits was highly significant through F2 and F3 generations. Heritability estimates in broad sense improved considerably for all traits from F2 to advanced F3 generations. The genotypic correlation between three earliness characters was highly significant negative with most yield traits in F2 pop. I. While genotypic correlation between the previous earliness traits with yield traits in F3 generation was positively non-significant except for lint percentage that was highly significant positive. Genotypic correlation was highly significant positive among most yield and yield component traits in both generations, which helps pedigree selection to achieve high fast genetic advance. The data of the genotypic correlation among studied characters in F2 and F3 generations in population II showed highly significant negative genotypic correlation between earliness traits in F2 generation, changed to highly significant positive in F3 generation after applying pedigree selection. The genotypic correlation between most yield characters and fiber properties showed highly significant positive correlation in population II in both generations. The superior ten families in F3 generation in both populations ranked on high yield, yield components and fiber quality showed higher means than that of F2, F3, better parent and check in most yield characters
Forages, Fiber and the Rumen
Authors: Allen Mike 
Published: February 2023

The goal of formulating diets for carbohydrates is to provide low fill, highly fermentable diets that result in consistant ruminal fermentation over time.
Forage fiber content of diets limit feed intake of high producing cows.
Rapidly fermented starch sources can decrease feed intake and efficiency of microbial protein production.
Forages with highly digestible NDF with a high ruminal turnover rate are most valuable for high producing cows.
Consideration of carbohydrate digestion characteristics when formulating diets is important to maximize energy intake and microbial protein production.
Comparison of the γ-Interferon ELISA and the Skin Test for the Detection of Sub-clinical Johne's Disease in Cattle
Authors: Robbe-Austerman Suelee , Stabel Judy , O'Conner Annette , Thoen Charles , Martin Barb 
Published: February 2023

The diagnosis of Johne's disease in cattle can be difficult. Cattle are often infected for years before they begin shedding Mycobacterium avium subsp. paratuberculosis in their feces or mount a detectable humoral immune response. Current diagnostic tests, serology and fecal culture, are effective diagnostic tests to detect animals in advanced stages of disease, but fail to reliably detect infection in young replacement animals.
However, it is thought that the majority of animals elicit a detectable cell mediated immune (CMI) response before fecal shedding occurs, and a detectable humoral immune response after fecal shedding. Therefore, it is important to evaluate CMI diagnostic tests as potential tools to use in the detection of subclinical infection, especially in replacement populations. There are two common methods for evaluating the CMI response to M. paratuberculosis, skin testing and the γ-interferon (IFN-γ) test.
In the past, skin testing has not been viewed favorably in the literature. This may be in part due to the use of a gold standard (either fecal/tissue culture) to determine Johne's-positive status of individual animals. If the CMI response occurs before lesions are detected or organisms are at high enough concentrations to culture, then the skin test would be viewed as lacking specificity. In addition, no studies are published that carefully evaluate the skin test in known negative populations.
Although the IFN-γ has worked well in research settings, field studies must be done to evaluate the usefulness of this test in production settings. Experience and research with the skin test and IFN-γ ELISA for the detection of Mycobacterium bovis has shown there is high agreement between the two tests. Therefore, our objectives in this study were to evaluate the agreement between the IFN-γ and skin test for the detection of Johne's disease, and to see if these tests detect the disease status of young animal populations.
When the Lab Reports Come Back, "Abortion: Cause Undetermined" - What Next?
Authors: Schlafer Donald H. 
Published: February 2023

How frustrating and how far too common it is that after a "full" work-up of a case of bovine abortion one ends up with a final report saying, "Abortion: Cause Undetermined". Unfortunately, we frequently think of abortions as the outcome of in utero microbial infections and that submission of samples to a lab should result in determination of cause. In most labs, testing normally will consist of bacterial, viral or fungal culture, serology and gross and histopathology to scrutinize fetal and maternal tissues for clues of cause for the abortion. Endocrinology, toxicology or genetic testing of submitted samples are rarely done and, too frequently, thorough medical examinations of the dam or herdmates are lacking.
Solutions for complex clinical problems usually involve a "team effort" approach and failures can result anywhere along this chain. In practical terms laboratory testing is not perfect and our ability to clinically investigate many aspects of pregnancy is limited, if not impossible!
When reports come back with no cause determined, the question, "what next?" quickly becomes "what are we missing?"
Lists of differential diagnoses for non-infectious causes tend to be shorter, less clear-cut, and less often considered than differentials for infectious bovine abortion. We must, however, also include the possibility that some abortions are caused by infectious agents which are missed. This paper addresses non-infectious conditions that potentially could account for some of the missed, or cause-unknown abortions. Six subject areas are discussed.• Failure to detect infectious causes.• Conditions that disrupt essential supporting endocrine systems.• Unrecognized endometrial or cervical diseases.• Direct or indirect effects of toxic compounds.• Underlying genetic abnormalities.• Potential immune-mediated conditions.
Maedi-Visna (Ovine Progressive Pneumonia) Management Strategies
Authors: Knight Anthony P. 
Published: February 2023

Maedi-visna (ovine progressive pneumonia) is a lentivirus-induced lymphoid-proliferative disease syndrome of sheep characterized by chronic progressive pneumonia, encephalopathy, swollen joints causing lameness, and indurative lymphocytic mastitis. The primary mode of transmission is via the colostrum from infected ewes and to a lesser extent by management conditions that favor close proximity of sheep to one another. Seroprevalence to range from 19-97%, according to North American and European serological surveys of sheep.
Affected sheep typically show progressive weight loss, and dyspnea due to lymphocytic infiltration of the lungs. Neurological signs leading to paralysis, swollen joints causing lameness, and palpably hard, unproductive udders or "hard bag" may occur concurrently or separately in affected sheep. Once infected with the ovine lentivirus, antibodies are produced to the virus that do not confer resistance to the disease, which is ultimately fatal. Definitive diagnosis of maedi-visna can be made from the clinical signs, histopathology, and AGID and/or the ELISA tests that detect the presence of circulating antibody. The ELISA test has greater sensitivity and specificity than the AGID test.
Maedi-visna is best managed by eradicating the virus. Eradication strategies include removing new born lambs at birth from their infected dams before they can suckle colostrum, and then raising them in isolation. Serological testing of all sheep over three years of age and culling infected animals and their progeny will reduce seroprevalence in the flock. Repeated annual testing is required until the herd has at least two negative herd tests.
Penetration of Ceftiofur into Sterile versus Mannheimia Haemolytica-Infected Tissue Chambers in Beef Calves after Subcutaneous Administration of Ceftiofur Crystalline Free Acid Sterile Suspension in the Ear Pinna
Authors: Washburn K. E. , Johnson R. , Clarke C. R. , Anderson K. , Lucas M. , Bryson W. , Robinson J. , Dame K. , Hubbard V. , Callahan K. , Robb E. 
Published: February 2023

Ceftiofur is a third-generation cephalosporin approved in the US for the treatment of bovine respiratory disease in beef and dairy cattle. Ceftiofur crystalline free acid sterile suspension (CCFA-SS) is a single administration product developed as an extension to the ceftiofur product line. The efficacy of a single dose of CCFA-SS administered subcutaneously in the neck was established in several clinical field studies. However, this route of administration was not acceptable for registration because of extended residues at the injection site. Consequently, the middle third of the posterior aspect of the ear pinna was selected as an alternative site of administration, and pilot studies were conducted to investigate the duration of efficacy resulting from a high concentration formulation. These studies indicated that administration of3.0 mg CE/lb (6.6 mg CE/kg) was efficacious, suggesting that therapeutic concentrations of ceftiofur and its active metabolite, desfuroylceftiofur, were maintained in interstitial fluids at sites of infection for prolonged periods. Bacterial infections of soft tissues, such as the lung, cause interstitial fluid composition changes which may affect the distribution of antibacterials or their in vitro activity in the target tissue. Evaluation of drug concentrations in intersitial fluid poses significant sampling difficulties; however, several studies have employed perforated tissue chambers implanted subcutaneously to characterize interstitial fluid drug concentrations in cattle.
The objectives of this study were to evaluate the plasma and tissue chamber fluid pharmacokinetics of ceftiofur and desfuroylceftiofur after subcutaneous administration of CCFA-SS in the ear pinnae of healthy feedlot calves, and to compare the penetration of ceftiofur and related metabolites into sterile versus Mannheimia haemolyica infected tissue chambers.