bluuebunny/crossref_metadata_2025_split huggingface dataset:
Evidence for a functional interaction between the respiratory syncytial virus fusion and attachment proteins in the envelope of infectious virus particles
Authors: Huong Tra Nguyen , Tan Boon Huan , Sugrue Richard J. 
Published: December 2022

AbstractWe have examined the interaction between the respiratory syncytial virus (RSV) F and G proteins on the surface of infected cells during multiple cycle infection using a low multiplicity of infection (MOI) model, and on the surface of virus particles that were isolated from infected cells. A combination of the proximity ligation assay (PLA) and confocal microscopy was used to demonstrate the interaction between the F and G proteins within the virus filaments on infected cells. Co-precipitation of the F and G proteins was confirmed using detergent extracts prepared from infected cells and in detergent extracts prepared from purified virus particles. The influence of the G protein in mediating virus spread in the low MOI model was further examined using the recombinant virus isolates rg224RSV (that expresses all virus proteins) and rg224RSV-ΔG (which does not express the G protein). While cells could be initially infected by both viruses, the rg224RSV-ΔG virus exhibited severely impaired localised virus transmission in the multiple cycle infection assay. Collectively these data provide evidence that the F and G proteins interact within the envelope of RSV particles, and suggests that this interaction may promote virus transmission. The interaction between these proteins in a single protein complex represents a potential new target for the development of antivirus strategies and in the development of RSV vaccine candidates.
SNARE protein tomosyn regulates dense core vesicle composition but not exocytosis in mammalian neurons
Authors: Subkhangulova Aygul , Gonzalez-Lozano Miguel A. , Groffen Alexander J. A. , van Weering Jan R. T. , Smit August B. , Toonen Ruud F. , Verhage Matthijs 
Published: December 2022

AbstractTomosyn is a large, non-canonical SNARE protein proposed to act as a competitive inhibitor of SNARE complex formation in vesicle exocytosis. In the brain, tomosyn inhibits fusion of synaptic vesicles (SVs), whereas its role in the fusion of neuropeptide-containing dense core vesicles (DCVs) is unknown. Here, we addressed this question using a new mouse model allowing conditional deletion of tomosyn (Stxbp5) and its paralogue tomosyn-2 (Stxbp5l), and an assay that detects DCV exocytosis with single vesicle resolution in primary hippocampal neurons. Surprisingly, loss of both tomosyns did not affect DCV exocytosis but resulted in a strong reduction of intracellular levels of many DCV cargos, most prominently brain-derived neurotrophic factor (BDNF), granin VGF and prohormone convertase PCSK1. Reduced levels of DCV cargos were paralleled by decreased DCV size and impaired mRNA expression of the corresponding genes. We conclude that tomosyns regulate neuropeptide and neurotrophin secretion via control of DCV cargo production, and not at the step of cargo release. Our findings suggest a differential effect of tomosyn on the two main secretory pathways in mammalian neurons and argues against a conserved role of tomosyn as competitive inhibitor of SNARE complex formation.
AGFPsplicing reporter in acoilinmutant background reveals links between alternative splicing, siRNAs and coilin function inArabidopsis thaliana
Authors: Kanno Tatsuo , Chiou Phebe , Wu Ming-Tsung , Lin Wen-Dar , Matzke Antonius , Matzke Marjori 
Published: December 2022

AbstractCoilin is a scaffold protein essential for the structure of Cajal bodies, which are nucleolar-associated, nonmembranous organelles that coordinate the assembly of nuclear ribonucleoproteins (RNPs) including spliceosomal snRNPs. To learn more about coilin functions and pathways in plants, we conducted a genetic suppressor screen using acoilin (coi1)mutant inArabidopsis thalianaand performed an immunoprecipitation-mass spectrometry analysis on coilin protein. Thecoi1mutations modify alternative splicing of aGFPreporter gene, resulting in a ‘hyper-GFP’ phenotype in youngcoi1seedlings relative to the intermediate wild-type level. As shown here, this hyper-GFP phenotype is extinguished in oldercoi1seedlings by posttranscriptional gene silencing triggered by siRNAs derived from aberrant splice variants ofGFPpre-mRNA. In thecoi1suppressor screen, we identified suppressor mutations in WRAP53, a putative coilin-interacting protein; SMU2, a predicted splicing factor; and ZC3HC1, an incompletely characterized zinc finger protein. These suppressor mutations return the hyper-GFP fluorescence of youngcoi1seedlings to the intermediate wild-type level. Additionally,zc3hc1 coi1mutants display more extensiveGFPsilencing and elevated levels ofGFPsiRNAs, suggesting the involvement of wild-type ZC3HC1 in siRNA biogenesis or stability. The immunoprecipitation-mass spectrometry analysis reinforced coilin’s roles in pre-mRNA splicing, nucleolar chromatin structure, and rRNA processing. Coilin’s participation in these processes, at least some of which incorporate small RNAs, supports the hypothesis that coilin acts as a chaperone for small noncoding RNAs. Our study demonstrates the usefulness of theGFPsplicing reporter for investigating alternative splicing, ribosome biogenesis, and siRNA-mediated silencing in the context of coilin function.
Pregnancy preparation amongst women and their partners in the UK; how common is it and what do people do?
Authors: Stewart Catherine Louise , Hall Jennifer Anne 
Published: December 2022

AbstractBackgroundPregnancy preparation, to establish a healthy lifestyle within the preconception period, has been shown to reduce adverse maternal and neonatal outcomes. Despite its importance, we know very little about if and how people prepare for pregnancy in the UK.MethodsAs part of the P3 study, women in the UK were invited to complete an online survey about pregnancy preferences, including the Desire to Avoid Pregnancy (DAP) Scale. 274 participants were currently trying, thinking, or maybe thinking about getting pregnant and were asked about pregnancy preparations. The changes that women, and their partners, made in preparation for pregnancy, reasons for not preparing, and associations with sociodemographics were investigated in univariate and multivariate analyses.ResultsOf the 274 women, less than half (n=134, 49%) reported making any changes in preparation for pregnancy, with the most common changes being “eating healthier” (55%) and “folicacid” (54%). The main reason for not preparing was “only thinking about getting pregnant” (38%). 92 women answered questions about partner preparations; only 24% of partners were preparing, with the most common changes being “eating healthier” (64%) and “reducing alcohol” (50%). The main reason for partners not preparing was “already healthy” (51%). DAP score was the only significant factor affecting pregnancy preparation; every one- point increase in DAP score reduced the odds of a woman preparing for pregnancy by 78% (OR 0.22, 95%CI 0.15-0.34).ConclusionInterventions addressing pregnancy preparation for women, and their partners, are needed. These strategies should target women thinking about pregnancy, to ensure the full benefits of preconception care are received.
Polygenic risk scores for nicotine use and family history of smoking are associated with smoking behaviour
Authors: Foo Jerome C. , Streit Fabian , Frank Josef , Zacharias Norman , Zillich Lea , Sirignano Lea , Völker Maja P. , Nürnberg Peter , Wienker Thomas , Wagner Michael , Nöthen Markus , Nothnagel Michael , Walter Henrik , Lenz Bernd , Spanagel Rainer , Kiefer Falk , Winterer Georg , Rietschel Marcella , Witt Stephanie H. 
Published: December 2022

AbstractIntroductionFormal genetics studies show that smoking is influenced by genetic factors; exploring this on the molecular level can offer deeper insight into the etiology of smoking behaviours.MethodsSummary statistics from the GWAS and Sequencing Consortium of Alcohol and Nicotine (GSCAN) Consortium were used to calculate polygenic risk scores (PRS) in a sample of ∼2,200 smokers/never-smokers. The association of PRS for Smoking Initiation (i.e. Lifetime Smoking; SI-PRS) with smoking status, and PRS for Cigarettes per Day (CpD-PRS) and Smoking Cessation (SC-PRS) with Fagerström Test for Nicotine Dependence (FTND) score were examined, as were distinct/additive effects of parental smoking on smoking status.ResultsSI-PRS explained 6.65% of variance (Nagelkerke-R2) in smoking status (p=1.71×10−24). In smokers, CpD-PRS (R2=3.15%, p=1.82×10−8) and SC-PRS (R2=2.01%; p=7.18×10−6) were associated with FTND score. Parental smoking alone explained R2=3.06% (p=2.43×10−12) of smoking status, and 1.39% when added to the most informative SI-PRS model (total R²=8.04%).ConclusionThese results show the potential utility of molecular genetic data for research investigating smoking prevention. The fact that PRS explains more variance than family history highlights progress from formal to molecular genetics; the overlap and increased predictive value when using both suggests the importance of combining these approaches.ImplicationsThis study underlines the value of using PRS to predict smoking status/behaviour. It highlights the importance of molecular genetic methods in research investigating smoking prevention and points to the necessity of combining family history and molecular genetic data.
Adopting and adapting sustainability accounting: fit and faith in a family business
Authors: Maughan Rebecca 
Published: December 2022

PurposeThe purpose of this paper is to provide a theoretically informed analysis of the evolution of environmental management accounting (EMA) and social and environmental reporting (SER), and the accompanying development of a sustainability programme, in a large family-owned, unlisted corporation.Design/methodology/approachA longitudinal case study based on semi-structured interviews and documentary data was conducted. The main periods of fieldwork were carried out in 2007 and between 2010 and 2012. Sustainability reports were collected until 2019 when SER appeared to cease. The case analysis draws on the concepts of organisational identity (OI) and internal legitimacy (IL) to examine the decision-making and actions of a range of key organisational actors as they engage with EMA and SER.FindingsThe study demonstrates that a gap between an organisation’s identity claims (“who we are”) and its enacted identity (“what we do”) can enable the adoption of constitutive, performative and representational EMA and SER. It illuminates the nature of the role of key actors and organisational dynamics, in the form of OI and IL, in adapting these practices. It also demonstrates that, in giving meaning to the concept of sustainability, organisational actors can draw on their organisation’s identity and construct the comprehensibility of an organisational sustainability programme.Research limitations/implicationsMore empirical work is needed to examine the applicability of OI and IL to other settings. It would also be beneficial to examine the potential for OI work to allow organisations to change and reinvent themselves in response to the evermore pressing environmental crisis and the role, if any, of EMA in this process.Originality/valueThe study enriches our understanding of why and how EMA and SER evolve by demonstrating that paying attention to OI and IL can provide further insight into the decision-making and actions of organisational members as they recognise, evaluate, support and cease these practices.
Digitalization in accounting–Warmly embraced or coldly ignored?
Authors: Jans Mieke , Aysolmaz Banu , Corten Maarten , Joshi Anant , van Peteghem Mathijs 
Published: December 2022

PurposeThe Accounting Information Systems (AIS) research field emerged around 30 years ago as a subfield of accounting but is at risk to develop further as an isolated discipline. However, given the importance of digitalization and its relevance for accounting, an amalgamation of the parent research field of accounting and the subfield of accounting information systems is pivotal for continuing relevant research that is of high quality. This study empirically investigates the distance between AIS research that is included in accounting literature and AIS research that prevails in dedicated AIS research outlets.Design/methodology/approachTo understand which topics define AIS research, all articles published in the two leading AIS journals since 2000 were analyzed. Based on this topical inventory, all AIS studies that were published in the top 16 accounting journals, also since 2000, are identified and categorized in terms of topic, subtopic and research methodology. Next, AIS studies published in the general accounting field and AIS studies published in the AIS field were compared in terms of topics and research methodology to gain insights into the distance between the two fields.FindingsThe coverage of AIS topics in accounting journals is, to no small extent, concentrated around the topics “information disclosure”, “network technologies” and “audit and control”. Other AIS topics remain underrepresented. A possible explanation might be the focus on archival studies in accounting outlets, but other elements might play a role. The findings suggest that there is only a partial overlap between the parent accounting research field and the AIS subfield, in terms of both topic and research methodology diversity. These findings suggest a considerable distance between both fields, which might hold detrimental consequences in the long run, if no corrective actions are taken.Originality/valueThis is the first in-depth investigation of the distance between the AIS research field and its parent field of accounting. This study helped develop an AIS classification scheme, which can be used in other research endeavors. This study creates awareness of the divergence between the general accounting research field and the AIS subfield. Given the latter's relevance to the accounting profession, isolation or deterioration of the AIS research must be avoided. Some actionable suggestions are provided in the paper.
Shareholder sentiment at general meetings: speculating on colonialism
Authors: Power Sean Bradley , Brennan Niamh M. 
Published: December 2022

PurposeAnnual general meetings have been variously described as dull rituals for accountability versus entertaining theatre at the expense of accountability. The research analyses director and shareholder participation and dialogic interactions at annual and extraordinary general meetings of Cecil Rhodes' British South Africa Company (BSAC). The BSAC was incorporated under a royal charter in 1889 in return for power to exploit a huge territory, Rhodesia/now Zimbabwe. The BSAC's administration ceased in 1924/25. Thus, the BSAC had a dual mandate as a private for-profit listed company and to occupy and develop the territories on behalf of the British government.Design/methodology/approachThe article analyses 29 BSAC general meeting minutes, comprising 25 full sets of verbatim minutes between 1895 and 1925. The study adopts manual content analysis. First, the research adopts conversational analysis to analyse director and shareholder turn-taking and moves by approving and dissenting shareholders. Second, the study identifies and analyses incidents of shareholder sentiment from the shareholder turns/moves. Finally, the article assesses how shareholder sentiment changed throughout the period and whether the BSAC's share price reflected the shareholder sentiment.FindingsThe BSAC's general meetings were associated with the greater colonial project of building the British Empire. The authors find almost 1,500 incidents of shareholder sentiment. Directors and shareholders take roughly an equal number of turns (excluding shareholder sentiment). Ritual and ceremony dominate director and shareholder turns and moves, while accountability to shareholders was minimal. The BSAC share price spiked in the early years of the project, waning after that. Shareholder sentiment, both positive and negative, reflect the share price behaviour.Originality/valueA unique database of verbatim general meeting minutes records shareholders' reactions to what they heard in the form of sounding off through cheering, “hear, hears,” laughter and applause (i.e. shareholder sentiment).
Global collaboration in teaching root cause analysis with healthcare professional students
Authors: Hampe Holly , Frndak Diane , Kydonaki Claire 
Published: December 2022

PurposeThe purpose of this paper is to describe an example of how universities can develop unique synchronous educational opportunities to engage students in an online environment, especially since study abroad opportunities have slowed or halted due to the COVID-19 pandemic crisis.Design/methodology/approachTwo affiliated universities, in the United States and Scotland, selected a simulated root cause analysis (RCA) of a medical error for a collaborative activity.FindingsTogether the students analyzed the event within assumed roles of healthcare professions in a systematic way using the “Swiss Cheese Model,” identified opportunities for improvement, debriefed using the Promoting Excellence and Reflective Learning in Simulation (PEARLS) method and submitted a reflective assignment. The RCA resulted in several process improvement suggestions and positive feedback from the participants.Research limitations/implicationsThe approach was for graduate students from two affiliated universities, in the United States and Scotland, to simulate a RCA of a medical error as a collaborative activity.Practical implicationsThis example using simulation of healthcare roles demonstrates the application of international collaboration, problem-solving models and methodologies and recognition of the similarities and differences between the United Kingdom and the United States approaches to patient safety. Especially with the ongoing COVID-19 pandemic, the students' ability to internationally collaborate can be limited without such unique opportunities.Social implicationsThe students were provided with a side-by-side comparison of the patient safety efforts in each country and written and didactic material about an RCA and the Swiss cheese model.Originality/valueStudents come from different perspectives of health services administration, nursing and nursing leadership from Scotland and the United States to solve patient safety problems.
Analytical modeling of quality parameters in casting process – learning-based approach
Authors: Suthar Janak , Persis Jinil , Gupta Ruchita 
Published: December 2022

PurposeFoundry produces cast metal components and parts for various industries and drives manufacturing excellence all over the world. Assuring quality of these components and parts is vital for the end product quality. The complexity in foundry operations increases with the complexity in designs, patterns and geometry and the quality parameters of the casting processes need to be monitored, evaluated and controlled to achieve expected quality levels.Design/methodology/approachThe literature addresses quality improvement in foundry industry primarily focusing on surface roughness, mechanical properties, dimensional accuracy and defects in the cast parts and components which are often affected by numerous process variables. Primary data are collected from the experts working in sand and investment casting processes. The authors perform machine learning analysis of the data to model the quality parameters with appropriate process variables. Further, cluster analysis using k-means clustering method is performed to develop clusters of correlated process variables for sand and investment casting processes.FindingsThe authors identified primary process variables determining each quality parameter using machine learning approach. Quality parameters such as surface roughness, defects, mechanical properties and dimensional accuracy are represented by the identified sand-casting process variables accurately up to 83%, 83%, 100% and 83% and are represented by the identified investment-casting process variables accurately up to 100%, 67%, 67% and 100% respectively. Moreover, the prioritization of process variables in influencing the quality parameters is established which further helps the practitioners to monitor and control them within acceptable levels. Further the clusters of process variables help in analyzing their combined effect on quality parameters of casting products.Originality/valueThis study identified potential process variables and collected data from experts, researchers and practitioners on the effect of these on the quality aspects of cast products. While most of the previous studies focus on a very limited process variables for enhancing the quality characteristics of cast parts and components, this study represents each quality parameter as the function of influencing process variables which will enable the quality managers in Indian foundries to maintain capability and stability of casting processes. The models hence developed for both sand and investment casting for each quality parameter are validated with real life applications. Such studies are scarcely reported in the literature.
How risk-aversion level of return policies impact consumer trust in online shopping? An intercultural study in the Middle East
Authors: Askarifar Kazem , Dehbozorgi Yalda , Alsafi Ali 
Published: December 2022

Purpose
This study aims to examine the relationship between the risk-aversion level of return policies and customer trust in online shopping in three countries in the Middle East.

Design/methodology/approach
In the first step, the different types of return policies of online shoppers and the risk-aversion level were determined by surveying 18 online shops and interviewing 21 customers. The risk-aversion level of these policies was found in the experts’ panel. In the second step, the experiences of 573 consumers when facing seller’s return messages, perceived risk and consumer trust in three countries (UAE, Iraq and Iran) were collected through a questionnaire. Finally, the gathered data were analyzed using structural equation modeling.

Findings
The results indicated that risk-averse return policies taken by online vendors led to lower consumer trust. Moreover, customer perceived risk mediated the relationship between return policies’ risk-aversion level and trust in Iraq and Iran. At the same time, there was no significant association between return policies and perceived risk in UAE. In addition, perceived risk wholly mediated the relationship between return policies’ risk aversion and trust in vendors in Iran, but this role was minor in Iraq.

Originality/value
Among the intercultural studies, especially in the Middle East, this is one of the first studies based on the marketing and sales management in an online shopper’s supply chain. Moreover, the investigation of return policies in online commerce is another innovative aspect of the present paper.