bluuebunny/crossref_metadata_2025_split huggingface dataset:
Effects of adverse fertility-related factors on mitochondrial DNA in the oocyte: a comprehensive review
Authors: Zhang Wenying , Wu Fuju 
Published: March 2023

AbstractThe decline of oocyte quality has profound impacts on fertilization, implantation, embryonic development, and the genetic quality of future generations. One factor that is often ignored but is involved in the decline of oocyte quality is mitochondrial DNA (mtDNA) abnormalities. Abnormalities in mtDNA affect the energy production of mitochondria, the dynamic balance of the mitochondrial network, and the pathogenesis of mtDNA diseases in offspring. In this review, we have detailed the characteristics of mtDNA in oocytes and the maternal inheritance of mtDNA. Next, we summarized the mtDNA abnormalities in oocytes derived from aging, diabetes, obesity, and assisted reproductive technology (ART) in an attempt to further elucidate the possible mechanisms underlying the decline in oocyte health. Because multiple infertility factors are often involved when an individual is infertile, a comprehensive understanding of the individual effects of each infertility-related factor on mtDNA is necessary. Herein, we consider the influence of infertility-related factors on the mtDNA of the oocyte as a collective perspective for the first time, providing a supplementary angle and reference for multi-directional improvement strategies of oocyte quality in the future. In addition, we highlight the importance of studying ART-derived mitochondrial abnormalities during every ART procedure.
Honokiol acts as an AMPK complex agonist therapeutic in non-alcoholic fatty liver disease and metabolic syndrome
Authors: Tian Ruifeng , Yang Jinjie , Wang Xiaoming , Liu Shuaiyang , Dong Ruixiang , Wang Zhenya , Yang Zifeng , Zhang Yingping , Cai Zhiwei , Yang Hailong , Hu Yufeng , She Zhi-Gang , Li Hongliang , Zhou Junjie , Zhang Xiao-Jing 
Published: March 2023

Abstract
Background
Non-alcoholic fatty liver (NAFLD) and its related metabolic syndrome have become major threats to human health, but there is still a need for effective and safe drugs to treat these conditions. Here we aimed to identify potential drug candidates for NAFLD and the underlying molecular mechanisms.

Methods
A drug repositioning strategy was used to screen an FDA-approved drug library with approximately 3000 compounds in an in vitro hepatocyte model of lipid accumulation, with honokiol identified as an effective anti-NAFLD candidate. We systematically examined the therapeutic effect of honokiol in NAFLD and metabolic syndrome in multiple in vitro and in vivo models. Transcriptomic examination and biotin-streptavidin binding assays were used to explore the underlying molecular mechanisms, confirmed by rescue experiments.

Results
Honokiol significantly inhibited metabolic syndrome and NAFLD progression as evidenced by improved hepatic steatosis, liver fibrosis, adipose inflammation, and insulin resistance. Mechanistically, the beneficial effects of honokiol were largely through AMPK activation. Rather than acting on the classical upstream regulators of AMPK, honokiol directly bound to the AMPKγ1 subunit to robustly activate AMPK signaling. Mutation of honokiol-binding sites of AMPKγ1 largely abolished the protective capacity of honokiol against NAFLD.

Conclusion
These findings clearly demonstrate the beneficial effects of honokiol in multiple models and reveal a previously unappreciated signaling mechanism of honokiol in NAFLD and metabolic syndrome. This study also provides new insights into metabolic disease treatment by targeting AMPKγ1 subunit-mediated signaling activation.
Tumor cell integrin β4 and tumor stroma E-/P-selectin cooperatively regulate tumor growth in vivo
Authors: Genduso Sandra , Freytag Vera , Schetler Daniela , Kirchner Lennart , Schiecke Alina , Maar Hanna , Wicklein Daniel , Gebauer Florian , Bröker Katharina , Stürken Christine , Milde-Langosch Karin , Oliveira-Ferrer Leticia , Ricklefs Franz L. , Ewald Florian , Wolters-Eisfeld Gerrit , Riecken Kristoffer , Unrau Ludmilla , Krause Linda , Bohnenberger Hanibal , Offermann Anne , Perner Sven , Sebens Susanne , Lamszus Katrin , Diehl Linda , Linder Stefan , Jücker Manfred , Schumacher Udo , Lange Tobias 
Published: March 2023

Abstract
Background
The immunological composition of the tumor microenvironment has a decisive influence on the biological course of cancer and is therefore of profound clinical relevance. In this study, we analyzed the cooperative effects of integrin β4 (ITGB4) on tumor cells and E-/P-selectin on endothelial cells within the tumor stroma for regulating tumor growth by shaping the local and systemic immune environment.

Methods
We used several preclinical mouse models for different solid human cancer types (xenograft and syngeneic) to explore the role of ITGB4 (shRNA-mediated knockdown in tumor cells) and E-/P-selectins (knockout in mice) for tumor growth; effects on apoptosis, proliferation and intratumoral signaling pathways were determined by histological and biochemical methods and 3D in vitro experiments; changes in the intratumoral and systemic immune cell composition were determined by flow cytometry and immunohistochemistry; chemokine levels and their attracting potential were measured by ELISA and 3D invasion assays.

Results
We observed a very robust synergism between ITGB4 and E-/P-selectin for the regulation of tumor growth, accompanied by an increased recruitment of CD11b+ Gr-1Hi cells with low granularity (i.e., myeloid-derived suppressor cells, MDSCs) specifically into ITGB4-depleted tumors. ITGB4-depleted tumors undergo apoptosis and actively attract MDSCs, well-known to promote tumor growth in several cancers, via increased secretion of different chemokines. MDSC trafficking into tumors crucially depends on E-/P-selectin expression. Analyses of clinical samples confirmed an inverse relationship between ITGB4 expression in tumors and number of tumor-infiltrating leukocytes.

Conclusions
These findings suggest a distinct vulnerability of ITGB4Lo tumors for MDSC-directed immunotherapies.

Graphical Abstract
Effects of n-3 polyunsaturated fatty acid on metabolic status in women with polycystic ovary syndrome: a meta-analysis of randomized controlled trials
Authors: Zhou Jie , Zuo Wenting , Tan Yong , Wang Xudong , Zhu Meihong , Zhang Huili 
Published: March 2023

AbstractThis meta-analysis was conducted to summarize the effects of n-3 polyunsaturated fatty acid (n-3 PUFA) on metabolic status including insulin metabolism and lipid metabolism in women with polycystic ovary syndrome (PCOS) by randomized controlled trials (RCTs). Four mainstream databases including PubMed, Cochrane Library, Embase and Web of Science were searched from their inception to October 2021. The registration number of this study was CRD42021285233. The quality assessment was performed referring the Cochrane Risk of Bias Tool. Mean differences (MD) and 95% confidence intervals (CIs) were generated for continuous variables by meta-analysis. Subgroup analyses were performed based on study duration (≤ 8 weeks or > 8 weeks), the source of n-3 PUFA (marine derived or plant origins) and dosage of n-3 PUFA (≤ 1000 mg/d or > 1000 mg/d). Eventually, 11 RCTs reporting 816 patients were enrolled. Compared with control group, n-3 PUFA treatment decreased waist circumference (MD = -2.76, 95% CI: -3.82 to -1.69; p < 0.00001), fasting plasma glucose (MD = -3.91, 95% CI: -5.69 to -2.13; p < 0.0001), fasting insulin (MD = -2.45, 95% CI: -3.19 to -1.71; p < 0.00001), homeostatic model assessment of insulin resistance (MD = -0.45, 95% CI: -0.80 to -0.11; p = 0.01), triglyceride (MD = -9.33, 95% CI: -10.56 to -8.10; p < 0.00001), total cholesterol (MD = -12.32, 95% CI: -19.15 to -5.50; p = 0.0004), low-density lipoprotein cholesterol (MD = -10.53, 95% CI: -19.31 to -1.75; p = 0.02), and increase quantitative insulin sensitivity check index (MD = 0.01, 95% CI: 0.01 to 0.02; p < 0.00001), Adiponectin (MD = 1.46, 95% CI: 1.12 to 1.80; p < 0.00001) in PCOS patients. However, n-3 PUFA failed to change body weight, body mass index, high-density lipoprotein cholesterol, very low-density lipoprotein cholesterol and hs-CRP in the overall analysis. Further subgroup analyses showed that supplements of n-3 PUFA for more than 8 weeks is more conducive to improve the metabolic status in insulin resistance and lipid profiles. The meta-analysis demonstrates that n-3 PUFA may be an effective intervention for alleviating metabolic status in PCOS. Hence, we recommend PCOS patients replenish n-3 PUFA with duration > 8 weeks regardless of the source and the dosage to retard the pathogenesis of PCOS related metabolic diseases.
Relationships between circulating metabolites and facial skin aging: a Mendelian randomization study
Authors: Liu Zhengye , Mi Jiarui , Wu Huiling 
Published: March 2023

Abstract
Background
Blood metabolites are important to various aspects of our health. However, currently, there is little evidence about the role of circulating metabolites in the process of skin aging.

Objectives
To examine the potential effects of circulating metabolites on the process of skin aging.

Method
In the primary analyses, we applied several MR methods to study the associations between 249 metabolites and facial skin aging risk. In the secondary analyses, we replicated the analyses with another array of datasets including 123 metabolites. MR Bayesian model averaging (MR-BMA) method was further used to prioritize the metabolites for the identification of predominant metabolites that are associated with skin aging.

Results
In the primary analyses, only the unsaturation degree of fatty acids was found significantly associated with skin aging with the IVW method after multiple testing (odds ratio = 1.084, 95% confidence interval = 1.049–1.120, p = 1.737 × 10−06). Additionally, 11 out of 17 unsaturation-related biomarkers showed a significant or suggestively significant causal effect [p < 0.05 and > 2 × 10−4 (0.05/249 metabolites)]. In the secondary analyses, seven metabolic biomarkers were found significantly associated with skin aging [p < 4 × 10−4 (0.05/123)], while six of them were related to the unsaturation degree. MR-BMA method validated that the unsaturation degree of fatty acids plays a dominant role in facial skin aging.

Conclusions
Our study used systemic MR analyses and provided a comprehensive atlas for the associations between circulating metabolites and the risk of facial skin aging. Genetically proxied unsaturation degree of fatty acids was highlighted as a dominant factor correlated with the risk of facial skin aging.
Collaborative learning, scaffolding-based instruction, and self-assessment: impacts on intermediate EFL learners’ reading comprehension, motivation, and anxiety
Authors: Ahmed Abdel-Al Ibrahim Khaled , Cuba Carbajal Nestor , Zuta Manuel Enrique Chenet , Bayat Sania 
Published: March 2023

AbstractThis research set to examine the impacts of collaborative learning (CL), scaffolding instruction, and self-assessment on reading anxiety, reading motivation, and reading comprehension of Iranian EFL learners. Based on Preliminary English Test (PET) results, a sample of 58 students was chosen to represent the study’s sample subject out of a total of 71 participants. Running a convenience sampling technique, two equal groups (the control and experimental) were chosen at random from among them. Afterward, both groups completed pretests to gauge their reading motivation, anxiety, and comprehension. Two groups were then treated with a variety of treatments. Learners in the experimental group got scaffolding instruction, self-assessment, and CL as treatment, whereas the control group received typical instructor-based teaching and assessment. Later, both groups took the post-test for reading anxiety, reading comprehension, and reading motivation after the treatment. According to the one-way ANCOVA analysis, the post-test results for the two groups were different. Based on the data analysis, the experimental group outdid the control group in reading anxiety, reading motivation, and reading comprehension. It was concluded that Iranian EFL learners were able to develop both their reading comprehension and reading motivation while experiencing less reading anxiety thanks to CL, self-assessment, and scaffolding instruction. Finally, the conclusions, implications, limitations, and suggestions for further studies were provided.
Releasing high positive end-expiratory pressure to a low level generates a pronounced increase in particle flow from the airways
Authors: Broberg Ellen , Pierre Leif , Fakhro Mohammed , Malmsjö Malin , Lindstedt Sandra , Hyllén Snejana 
Published: March 2023

Abstract
Objectives
Detecting particle flow from the airways by a non-invasive analyzing technique might serve as an additional tool to monitor mechanical ventilation. In the present study, we used a customized particles in exhaled air (PExA) technique, which is an optical particle counter for the monitoring of particle flow in exhaled air. We studied particle flow while increasing and releasing positive end-expiratory pressure (PEEP). The aim of this study was to investigate the impact of different levels of PEEP on particle flow in exhaled air in an experimental setting. We hypothesized that gradually increasing PEEP will reduce the particle flow from the airways and releasing PEEP from a high level to a low level will result in increased particle flow.

Methods
Five fully anesthetized domestic pigs received a gradual increase of PEEP from 5 cmH2O to a maximum of 25 cmH2O during volume-controlled ventilation. The particle count along with vital parameters and ventilator settings were collected continuously and measurements were taken after every increase in PEEP. The particle sizes measured were between 0.41 µm and 4.55 µm.

Results
A significant increase in particle count was seen going from all levels of PEEP to release of PEEP. At a PEEP level of 15 cmH2O, there was a median particle count of 282 (154–710) compared to release of PEEP to a level of 5 cmH2O which led to a median particle count of 3754 (2437–10,606) (p < 0.009). A decrease in blood pressure was seen from baseline to all levels of PEEP and significantly so at a PEEP level of 20 cmH2O.

Conclusions
In the present study, a significant increase in particle count was seen on releasing PEEP back to baseline compared to all levels of PEEP, while no changes were seen when gradually increasing PEEP. These findings further explore the significance of changes in particle flow and their part in pathophysiological processes within the lung.
Ritualized world relations: a Confucian critique of Rosa’s limitations on resonance
Authors: Sigurðsson Geir , D’Ambrosio Paul J. 
Published: March 2023

AbstractHartmut Rosa argues that our modern and post-modern societies can be understood through the notion of dynamic stabilization—institutions require growth to maintain themselves. Part of the impetus behind the acceleration that drives dynamic stabilization is the desire to make the world more available, attainable, and accessible. On both the institutional and individual levels, this is translated into making the world more within our reach, more engineerable, predictable, and controllable. Paradoxically, success in these areas is often accompanied by the world becoming increasingly silent, cold, and unresponsive. We feel alienated or that our world relation has failed. Rosa’s solution is to reestablish resonance with the world. In this paper, we argue that his notion of resonance depends on a degree of atomic agency that muffles its own efficacy. The Confucian notion of ritual offers a more dispersed notion of agency. Rather than seeing oneself, others, and the world as distinct agents or indivisible entities, a ritualized approach sees them as mutually constitutive. It is true even on the level of agency, which drastically changes our relationship with the world.
Simultaneous colonic metastasis of advanced gastric cancer: a case report
Authors: Shima Takafumi , Arita Asami , Sugimoto Satoshi , Takayama Shoichi , Kawaguchi Nao , Imai Yoshiro , Kitahara Tomohiro , Maeda Tamaki , Okuda Junji 
Published: March 2023

Abstract
Background
Although distant metastasis in gastric cancer can be present at the time of the initial diagnosis, colonic metastasis is extremely rare. This report describes a case of simultaneous colonic metastasis of advanced gastric cancer.

Case presentation
The patient was a 78-year-old woman with nausea and epigastric pain. Upper gastrointestinal endoscopy revealed an advanced invasive ulcerative tumor in the lesser curvature of the stomach extending from the anterior to the middle portion. Colonoscopy revealed a 4-mm polyp-like lesion in the mid-transverse colon; therefore, a polypectomy was performed. Both gastric and colonic tumors showed poorly differentiated adenocarcinoma with signet ring cell carcinoma. After providing informed consent, the patient underwent a total gastrectomy. Histologic examination showed similar morphologic features of both gastric and colonic tumors. Immunohistochemistry staining showed that these tumor cells were positive for cytokeratin (CK) 7 and negative for CK20.

Conclusions
This was an extremely rare case of simultaneous colonic metastasis of advanced gastric cancer. Because missed metastasis can result in a poorer prognosis, we propose a systemic search including colonoscopy for patients with advanced gastric cancer, especially cases involving poorly differentiated adenocarcinoma or signet ring cell carcinoma.
The mourning process and its importance in mental illness: a psychoanalytic understanding of psychiatric diagnosis and classification
Authors: Gibbons Rachel 
Published: March 2023

SUMMARYThis article brings together the psychiatric and psychoanalytic views of mental illness to deepen the understanding of mental disorder. The intention is to bring to the fore the importance of loss and mourning in clinical practice. Looking for the loss event that underpins the disorder helps determine therapeutic treatment options and increases the chance of authentic therapeutic engagement and recovery. The article summarises theory about the mourning process and discusses the relationship of loss and pathological mourning to mental illness. Fictitious case vignettes developed from years in psychiatric practice are used to illustrate how this relates to clinical practice and formulation.
Occupational class suicide risk: 12-year study of national coronial data
Authors: Burnett Alexander C.R. , Wong Quincy , Zeritis Stephanie , Deady Mark , Torok Michelle 
Published: March 2023

BackgroundPrevious research showed that the Global Financial Crisis (GFC) was associated with a widening disparity in suicide rates between lower-class occupations and the highest-class occupations in Australia. There has been no research investigating whether this trend continued post-GFC.AimsThis study aimed to investigate suicide rates by occupational class among employed Australians aged 15 years and over, between 2007 and 2018.MethodA population-level retrospective mortality study was conducted using data from the National Coronial Information System. Adjusted suicide rates were calculated over the period 2007 to 2018. Negative binomial regression models were used to assess the relationship between occupational class, gender and time, comparing post-GFC years (2010–2012, 2013–2015 and 2016–2018) with GFC years (2007–2009).ResultsRelative to the GFC period of 2007–2009, a significant reduction in suicide disparity between managers and other occupation groups was only observed among male labourers (rate ratios (RR) = 0.65, 95% CI 0.49–0.86) and male technicians/trades workers (RR = 0.73, 95% CI 0.56–0.96) for the period 2013–2015.ConclusionSkilled manual and lower-skilled occupational classes remain at elevated risk of suicide in Australia. While a decreasing divergence in suicide rates was only observed between labourer and manager occupational classes post-GFC, this trend was not maintained over the later part of the study period (2016–2018). There is a need to further understand the relationship between contextual factors associated with suicide among the employed population, especially during periods of economic downturn.
Middle Meningeal Artery Embolization Versus Conventional Management for Patients With Chronic Subdural Hematoma: A Systematic Review and Meta-Analysis
Authors: Sattari Shahab Aldin , Yang Wuyang , Shahbandi Ataollah , Feghali James , Lee Ryan P. , Xu Risheng , Jackson Christopher , Gonzalez L. Fernando , Tamargo Rafael J. , Huang Judy , Caplan Justin M. 
Published: March 2023

BACKGROUND:The results from studies that compare middle meningeal artery (MMA) embolization vs conventional management for patients with chronic subdural hematoma are varied.OBJECTIVE:To conduct a systematic review and meta-analysis on studies that compared MMA embolization vs conventional management.METHODS:Medline, PubMed, and Embase databases were searched. Primary outcomes were treatment failure and surgical rescue; secondary outcomes were complications, follow-up modified Rankin scale > 2, mortality, complete hematoma resolution, and length of hospital stay (day). The certainty of the evidence was determined using the GRADE approach.RESULTS:Nine studies yielding 1523 patients were enrolled, of which 337 (22.2%) and 1186 (77.8%) patients received MMA embolization and conventional management, respectively. MMA embolization was superior to conventional management for treatment failure (relative risk [RR] = 0.34 [0.14-0.82],P= .02), surgical rescue (RR = 0.33 [0.14-0.77],P= .01), and complete hematoma resolution (RR = 2.01 [1.10-3.68],P= .02). There was no difference between the 2 groups for complications (RR = 0.93 [0.63-1.37],P= .72), follow-up modified Rankin scale >2 (RR = 0.78 [0.449-1.25],P= .31), mortality (RR = 1.05 [0.51-2.14],P= .89), and length of hospital stay (mean difference = −0.57 [−2.55, 1.41],P= .57). For MMA embolization, the number needed to treat for treatment failure, surgical rescue, and complete hematoma resolution was 7, 9, and 3, respectively. The certainty of the evidence was moderate to high for primary outcomes and low to moderate for secondary outcomes.CONCLUSION:MMA embolization decreases treatment failure and the need for surgical rescue without furthering the risk of morbidity and mortality. The authors recommend considering MMA embolization in the chronic subdural hematoma management.