bluuebunny/crossref_metadata_2025_split huggingface dataset:
Microbiota from Intestinal Gut of Dawkinsia filamentosa (Valenciennes,1844) as Indicator of Environmental Condition
Authors: Muthusamy Kanthimathi , Sajen Shahul Hameed , Sharmila Syed Mohammed Ibrahim , Murugan Priyanga , Ramaiah Soranam 
Published: April 2023

Aims: To isolate and identify the gut associated micro-organisms present in the gastrointestinal tract of freshwater fish Dawkinsia filamentosa.
Place and Duration of Study: Sampling site: The fish samples were collected from the river Gadana, located at the foot hills of Western Ghats, Alwarkurichi, Tenkasi district, Tamil Nadu, India. Place: Sri Paramakalyani Centre for Environmental Sciences, Manonmaniam Sundaranar University, Azhwarkurichi, Tenkasi, Tamilnadu, India (Latitude 8o20’N; Longitutde 77o10’E). Duration: The research was done during December 2021 and April 2022.
Methodology: The fish samples were anaesthetized and surface sterilized followed by aseptic dissectionby using sterile scalpel and needle. The gut contents were removed and homogenized well and isolation of Gut micro biome was done by means of serial dilution. The identification of gastrointestinal bacteria was done by performing various biochemical tests such as Gram staining, Motility, Indole test, Methyl red test, Voges Proskauer test, Citrate utilization test, Triple sugar Iron test, Catalase test, Oxidase test, Urease test, Nitrate test, Starch hydrolysis and Carbohydrate Fermentation Test. The fungi present in gastrointestinal tract is identified with lacto phenol cotton blue staining followed by microscopic observations.
Results: The overall bacterial load of gastrointestinal material ranged from 47 x10-7 to 219 x10-5 cfu/g. A total of six bacterial strains and four fungi strains were isolated. Several biochemical tests were used to identify the bacterial isolates. The six bacterial isolates were tentatively identified as Pseudomonas sp., Aeromonas sp., Staphylococcus sp., Bacillus sp., Enterobacter sp., and Vibrio sp. and the four fungi species isolated from the gutwere Aspergillus flavus, Aspergillus niger, Beauveria basiana, and Penicillium sp.
Conclusion: The current research revealed that, Dawkinsia filamentosa has a variety of microbiota in its gut. Microbial species from freshwater environments may be able to augment fish feed with these bacteria in commercial aquaculture operations. Additionally, these findings have aided in the development of possible remedies, enhanced knowledge of host-microbe interactions in other vertebrates, and enhanced aquaculture practices. The benefits of identifying gut microbiota in fishes is, it helps to study about the host nutrient catabolism and immune defence mechanism against the disease-causing pathogens.
Effect of Organic Fertilizer on Initial Lettuce Development
Authors: Júnior Luiz Ferreira Coelho , Nascimento Ruan dos Santos , Castor Antônio Lucas Santos , Coelho Thaynara Cristine Moares , Borges Sérgio Cardoso 
Published: April 2023

Aims: The objective of this work was to evaluate the effects of the application of organic fertilizer in different concentrations under the initial development of lettuce plants.
Study Design: The experimental design was completely randomized with four replications of 5 plants for each treatment.
Place and Duration of Study: The present work was carried out in the premises of the Escola Família Agrícola de Ladeirinhas 'A' - EFAL, located in the Municipality of Japoatã in the State of Sergipe, Brazil. Between September 2022 - December 2022.
Methodology: Daily applications of organic fertilizer were performed in lettuce plants of Cv. Repolhuda, with a volume of 200 ml per plant, in the morning and afternoon, except for days with precipitation. To evaluate the effect of organic fertilizer, different concentrations 0% (control) were used. 5%, 10% and 20%. The following variables, fresh mass, number of leaves, plant height and root system length were analyzed.
Results: The results obtained with the application of organic fertilizer, showed that the plants that were fertilizer showed better initial development of lettuce Repolhuda, when compared with plants that did not receive the application of fertilizer for most of the variables analyzed in this study, and the concentration of 20% presented the best result.
Conclusion: The results obtained with the application of organic fertilizer, showed that the plants that were fertilized showed better initial development of lettuce.
The Impact of COVID on Microbiology Examination Result with its Further analysis based on Student’s gender and Place of Residence
Authors: Sunarti Lusia Sri , Arodes Evy Suryani , Diani Yusias Hikmat , Salshabila Ayu Aulia 
Published: April 2023

Aims: To study the impact of COVID on Microbiology examination result, and analyze it further based on the gender and place of residence.
Methods: Simple analysis conducted cross-sectionally based on the comparison of the result of final Microbiology exam result based on the student’s gender and place of residence. Data classified by the class of 2018 (Pre-COVID), 2019 (initial COVID) and 2020 (prolonged COVID).
Results: There is a pattern of decreasing exam scores, both in theory and practicum, when compared to exam results before COVID (class of 2018), during the initial of the COVID pandemic (class of 2019) and 1 year after COVID became a pandemic but not yet fully controlled (class of 2020).
Conclusion: COVID caused direct impact to medical education, in our context to Microbiology examination result.
Parental care decreases the survival of adults in the waterbug Abedus ovatus
Authors: Argaez Víctor , Munguía‐Steyer Roberto 
Published: May 2023

AbstractAmong animals, especially insects, care of offspring exclusively performed by males is rare. Paternal care increases survival of their offspring but may be costly for males. Our objective was to evaluate the potential survival costs of parental care in the waterbug Abedus ovatus where males only care their eggs. We conducted a capture‐mark‐recapture study and estimated apparent survival probabilities of parental males, non‐parental males and females in a Mexican waterbug population. In addition, we also estimated recapture rates and the transition rates between non‐parental males and parental males. Our results suggest that paternal care negatively affects the survival of A. ovatus since survival probability of parental males is lower than non‐parental males. The recapture probability was higher in non‐parental males than in parental males. The decreased probability of survival in parental males may be due to different factors. As males carry the eggs on their backs this could probably affect their swimming performance and therefore affect their ability to capture prey. Additionally, the physiological wear derived from the intense muscular activity when performing subaquatic parental care behaviors such as brood pumping, could deteriorate their condition and subsequently, their survival. Furthermore, parental males are more conspicuous to predators, especially in the later stages of egg development, which may increase mortality rates. Our results support the trade‐off between current and future reproduction and provide evidence of parental care survival costs in a model system where only males care the progeny.
‘The Unmeasurable’: Lessons from Cranbrook
Authors: Williams Tod 
Published: May 2023

AbstractTod Williams attended Cranbrook School for Boys and Brookside Elementary School. The campus was his playground and instilled in him a keen sense of enjoyment of its vistas, fountains and pools, its smells and its textures. He co‐founded his New York architecture studio with Billie Tsien in 1986, and returned to Cranbrook in the late 1990s to design and build the Natatorium.
Postgraduate Architectural Education In Situ
Authors: Massie William E 
Published: May 2023

AbstractFrom 2005 to 2017, William E Massie held the position of Architect‐in‐Residence/Head of Department at Cranbrook. Making is in Massie's lifeblood and his tenure was characterised by students making larger‐scale constructions. Dismantling the rather insular personal studio, Massie reintroduced a communal studio culture where students learn from each other and create in mutual association.
Unprompted: Open‐ended Investigations in the Choreography of Construction
Authors: Baker Emily 
Published: May 2023

AbstractInventor, fabricator, architect and educator Emily Baker laments the rarity of open‐ended architectural education that Cranbrook so exemplifies. She explains the bottom‐up approach to design, material fabrication and use of emerging digital tools she developed while attending Cranbrook in the 2010s. She works at full scale and encourages her students to do the same.
Methods of Inspiration: A Pedagogical Approach Based on Singularity
Authors: Lynch Peter 
Published: May 2023

AbstractA good architecture department facilitates students to find their own, personal architectural lexicon and ways and methods of seeing the world and creating within it. Peter Lynch, former Architect‐in‐Residence/ Head of Department at Cranbrook, describes the acts of creative midwifery that allowed him to inspire students and achieve successful architectures with them. Equally, a student working on similar architectural problems as their teacher can be a pedagogic resource for all concerned.
The Interior Within Hand's Reach: Tactile Proximity
Authors: Weinthal Lois 
Published: May 2023

AbstractOne of the fundamental skills of an architect is the ability to create void from mass. Another is the choreography of the changing relationship between bodies, objects and materiality, creating visual and haptic sensations. Lois Weinthal explores and teaches these particular architectural phenomena. Here she takes us on a journey illustrated by the recent work of her students at Toronto Metropolitan University.
Arrows: The Long Lines of Influence in Architecture
Authors: Reiser Jesse 
Published: May 2023

AbstractJesse Reiser reflects on the unexpected re‐emergence of craft within the disciplinary body of architecture: from Buck's Rock Work Camp in Connecticut during the summers of the 1970s, to Cranbrook Academy of Art, to the ongoing work of Reiser + Umemoto, the New York‐based practice he co‐founded.
Forming Action: The Subject in the Object
Authors: Deamer Peggy 
Published: May 2023

AbstractIn an autobiographical article, Professor Emerita of Yale University's School of Architecture Peggy Deamer records her experiences at architecture school and the epiphany moments that shifted her perception of the profession. This prompted her to question architects’ love of creating ‘perfect’ form and dwelling in the purely ocular‐centric and the cult of the lone genius.
The Agency of Making: An Anatomy of Practice‐based Pedagogy
Authors: Wilkins Gretchen 
Published: May 2023

AbstractCurrent Architect‐in‐ Residence/Head of Department at Cranbrook and Guest‐ Editor of this AD, Gretchen Wilkins describes contemporary Cranbrook and some of the Architecture Department's recent student work and preoccupations. Experimental zeal manifests itself everywhere as students search for their design‐selves through explorations into space, material and the city.
Bioinks for Space Missions: The Influence of Long‐Term Storage of Alginate‐Methylcellulose‐Based Bioinks on Printability as well as Cell Viability and Function
Authors: Windisch Johannes , Reinhardt Olena , Duin Sarah , Schütz Kathleen , Rodriguez Nuria Juliana Novoa , Liu Suihong , Lode Anja , Gelinsky Michael 
Published: May 2023

AbstractBioprinting is considered a key technology for future space missions and is currently being established on the International Space Station (ISS). With the aim to perform bioink production as a critical and resource‐consuming preparatory step already on Earth and transport a bioink cartridge “ready to use” to the ISS, the storability of bioinks is investigated. Hydrogel blends based on alginate and methylcellulose are laden with either green microalgae of the species Chlorella vulgaris or with different human cell lines including immortilized human mesenchymal stem cells, SaOS‐2 and HepG2, as well as with primary human dental pulp stem cells. The bioinks are filled into printing cartridges and stored at 4°C for up to four weeks. Printability of the bioinks is maintained after storage. Viability and function of the cells embedded in constructs bioprinted from the stored bioinks are investigated during subsequent cultivation: The microalgae survive the storage period very well and show no loss of growth and functionality, however a significant decrease is visible for human cells, varying between the different cell types. The study demonstrates that storage of bioinks is in principle possible and is a promising starting point for future research, making complex printing processes more effective and reproducible.
Uncovering Dynamic Edge‐Sites in Atomic Co−N−C Electrocatalyst for Selective Hydrogen Peroxide Production
Authors: Hu Jinwen , Shang Wenzhe , Xin Cuncun , Guo Jingya , Cheng Xusheng , Zhang Songlin , Song Suchan , Liu Wei , Ju Feng , Hou Jungang , Shi Yantao 
Published: May 2023

AbstractUnderstanding the nature of single‐atom catalytic sites and identifying their spectroscopic fingerprints are essential prerequisites for the rational design of target catalysts. Here, we apply correlated in situ X‐ray absorption and infrared spectroscopy to probe the edge‐site‐specific chemistry of Co−N−C electrocatalyst during the oxygen reduction reaction (ORR) operation. The unique edge‐hosted architecture affords single‐atom Co site remarkable structural flexibility with adapted dynamic oxo adsorption and valence state shuttling between Co(2−δ)+ and Co2+, in contrast to the rigid in‐plane embedded Co1−Nx counterpart. Theoretical calculations demonstrate that the synergistic interplay of in situ reconstructed Co1−N2‐oxo with peripheral oxygen groups gives a rise to the near‐optimal adsorption of *OOH intermediate and substantially increases the activation barrier for its dissociation, accounting for a robust acidic ORR activity and 2e− selectivity for H2O2 production.
Design, scaling and cost evaluations of circulating fluidized‐bed systems for biomass pyrolysis
Authors: Humbird David , Dutta Abhijit , Subramaniam Harihar , Mendez Juan 
Published: May 2023

AbstractTo generate updated and transparent capital cost estimates for biomass fast pyrolysis equipment, refinery fluidized catalytic cracking design and sizing principles are examined and extended to pyrolysis of woody biomass. Capital costs for the sized equipment are estimated with process‐industry software. A one‐dimensional flow simulation with pyrolysis kinetics is leveraged to validate the fluidization conditions and thermal energy balance. After successful sizing and a system cost estimate of $2.8 M (in 2016 US$) at the biorefinery scale of 1000 metric tons per day (MTD), these methods were exercised for even smaller equipment at the distributed pyrolysis scale with modifications to the process design constraints, and not directly comparable with the 1000 MTD case, arriving at capital cost estimates of $1.2 M for a 500 ton/day system and $0.9 M for a 200 ton/day system. It is noted that this work only estimates purchased equipment costs at the ±50% accuracy level; there are significant other custom factors applicable to each installation based on location, maturity, scale, complexities during installation, engineering and licensing costs, etc. that need to be added on to these estimates to derive investment costs.
A pharmacokinetics interaction study of antiplatelet agents aspirin and clopidogrel combined with dl‐3‐n‐butylphthalide in rats by liquid chromatography–tandem mass spectrometry
Authors: Li Linjie , Tan Jingxuan , Du Yujing , Li Xixuan , Lv Yongning , Zhai Xuejia 
Published: May 2023

AbstractA sensitive and specific high‐performance liquid chromatography–tandem mass spectrometry method has been developed to determine the pharmacokinetic interactions of the antiplatelet agents aspirin and clopidogrel combined with dl‐3‐n‐butylphthalide. For the determination of aspirin metabolite salicylic acid, clopidogrel inactive metabolite SR26334 and NBP prototype drug in rat plasma, plasma samples were prepared by precipitation of proteins using methanol containing 0.1% formic acid, followed by centrifugation. Chromatography was performed on a C18 column, eluting with a gradient of acetonitrile (with 0.1% formic acid)–water (with 0.1% formic acid). The detection adopted electrospray ion source and positive ion multiple reaction monitoring modes. The linear detection response range of salicylic acid is 80–80,000 ng/ml, and the linear detection response range of SR26334 and dl‐3‐n‐butylphthalide is 10–10,000 ng/ml. Our study revealed that dl‐3‐n‐butylphthalide affected the pharmacokinetics of aspirin and clopidogrel when administered to rats.
Peripheral vascular disease interventions: One size does not fit all
Authors: Khan Mahin R. , Jneid Hani 
Published: May 2023

Key Points
There are notable sex disparities in the outcomes of endovascular interventions for peripheral vascular disease (PVD).
Aggressive risk factor modification, follow‐up, and patient education are important to improve postprocedural outcomes.
Early detection may improve long‐term outcomes, pointing to the need for better screening in at‐risk populations.
Low‐Temperature NOx Removal Using Cu‐Hierarchical SAPO‐34 Catalysts with High Hydrothermal Stability
Authors: Silveira De Araujo Brenda Roberta , Onrubia‐Calvo Jon Ander , Pétaud Guillaume , Nieto‐Marquéz Antonio , Pereda‐Ayo Beñat , González Velasco Juan Ramón , Caravaca Angel , Gil Sonia 
Published: May 2023

AbstractCu‐hierarchical SAPO‐34‐based catalysts are targeted to allow an efficient gas diffusion through mesopores, improving NOx abatement efficiency at low temperature. A downside of mesopores, however, is that they could eventually reduce hydrothermal stability. To demonstrate the potential of novel catalytic systems, the NH3‐SCR catalytic activity of fresh and hydrothermally treated (HT, in air with 10 % H2O at 750 °C for 16 h) hierarchical catalysts was evaluated and compared with a Cu‐microporous catalyst. As expected, the catalytic performances of the hydrothermally treated Cu‐hierarchical catalysts did not surpass that of the microporous one at high temperatures. Nevertheless, their NH3‐SCR catalytic activity at low temperature overcame that of the microporous catalyst, which could be assigned to both: the increase of Cu2+ active sites after HT and the enhanced diffusion of reactants through the mesopores. This reduction of diffusion resistance by the formation of mesopores was further confirmed by the estimated effectiveness factor.
Design, Synthesis and Biological Evaluation of Histone Deacetylase Inhibitors Based on Pyrrolo[2,3‐d]pyrimidine and Pyrrolo[2,3‐b]pyridine Scaffolds
Authors: Mao Nian‐Dong , Gao Yuan , Dang Xia‐Wen , Duan Ji‐Long , Hui Zi , Che Hao , Xu Yueying , Zhang Hang , He Xingrui , Garrido Carmen , Ye Xiang‐Yang 
Published: May 2023

AbstractHistone deacetylases (HDACs) are validated targets for the development of anticancer drugs in epigenetics. We have designed and synthesized a series of novel HDAC inhibitors based on pyrrolo[2,3‐d]pyrimidine and pyrrolo[2,3‐b]pyridine scaffolds. Compound B3 {(E)‐3‐(4‐(((1‐(7H‐pyrrolo[2,3‐d]pyrimidin‐4‐yl)piperidin‐4‐yl)amino)methyl)phenyl)‐N‐hydroxyacrylamide} exhibits potent inhibitory activity against HDACs 1, 2, 3, 6, and 8 with IC50 values of 5.2, 6.0, 8.8, 4.4, and 173.0 nM, respectively. It exhibited potent antiproliferative effects against three tumour cell lines (IC50 values of 0.13, 0.37, and 1.11 μM, against MV‐4‐11, K562, and WSU‐DLCL‐2 cells, respectively) with two‐ to sixfold improvement relative to suberoylanilide hydroxamic acid (SAHA). Mechanistic studies on WSU‐DLCL‐2 cells revealed that B3 exhibits anticancer effects through the induction of G0/G1‐phase arrest and promotion of apoptosis. The results of this study warrant further investigation of this compound series for the treatment of hematological malignancy.
A Phase 1 First‐in‐Human Pharmacokinetic and Pharmacodynamic Study of JNJ‐64264681, a Covalent Inhibitor of Bruton's Tyrosine Kinase
Authors: Leu Jocelyn H. , Miao Xin , Shalayda Kevin , Coe Kevin J. , Kahnt Ariane , Wu Bonnie , Schnarr Megan , Franks Carol , Devlin James , Yang Tong‐Yuan , Palmer James A. , Zhang Mai , Zhou Honghui , Van Damme Wim , Smets Sophie , Aguilar Zuleima , Chaplan Sandra R. 
Published: May 2023

AbstractJNJ‐64264681 is an irreversible covalent inhibitor of Bruton's tyrosine kinase. This phase 1, first‐in‐human, 2‐part (single‐ascending dose [SAD]; multiple‐ascending dose [MAD]) study evaluated the safety, tolerability, pharmacokinetics (PK), and pharmacodynamics (PD; Bruton's tyrosine kinase occupancy [BTKO]) of JNJ‐64264681 oral solution in healthy participants. For SAD (N = 78), 6 increasing doses of JNJ‐64264681 (4–400 mg) or placebo were evaluated in fasted males. The effects of sex, food, and a capsule formulation were evaluated in separate cohorts. For MAD (N = 27), sequential cohorts of male and female participants received 36/100/200 mg JNJ‐64264681 once daily for 10 days. JNJ‐64264681 exposure (peak concentration; area under the concentration‐time curve) was less than dose proportional from 4 mg to 36 mg. Dose‐normalized area under the concentration‐time curves following the 36 mg and 100 mg doses were generally similar. The mean terminal half‐life was 1.6–13.2 hours. With multiple doses, steady state was achieved by day 2. A semimechanistic PK/PD model was developed using the first 5 SAD cohorts’ data to predict %BTKO in MAD cohorts. PK/PD model guided dose‐escalation, and all participants in the 200/400 mg single‐dose cohorts achieved ≥90% BTKO at 4 hours after dosing (peak) with prolonged occupancy. As BTKO data became available from MAD cohorts, it was found that observed BTKO data were consistent with model predictions. JNJ‐64264681 showed no safety signals of concern. Overall, safety, tolerability, PK, BTKO, and PK/PD modeling guided the rationale for dose selection for the subsequent first‐in‐patient lymphoma studies.
Rational Design of Trimetallic Sulfide Electrodes for Alkaline Water Electrolysis with Ampere‐Level Current Density
Authors: Li Jingwen , Wei Qing , Alomar Muneerah , Zhang Jian , Yang Shengxiong , Xu Xiaoyang , Lao Xinbin , Lan Minqiu , Shen Yuhan , Xiao Junwu , Tu Zhengkai 
Published: May 2023

AbstractElectrochemical water splitting is considered an environmentally friendly approach to hydrogen generation. However, it is difficult to achieve high current density and stability. Herein, we design an amorphous/crystalline heterostructure electrode based on trimetallic sulfide over nickel mesh substrate (NiFeMoS/NM), which only needs low overpotentials of 352 mV, 249 mV, and 360 mV to achieve an anodic oxygen evolution reaction (OER) current density of 1 A cm−2 in 1 M KOH, strong alkaline electrolyte (7.6 M KOH), and alkaline‐simulated seawater, respectively. More importantly, it also shows superior stability with negligible decay after continuous work for 120 h at 1 A cm−2 in the strong alkaline electrolyte. The excellent OER performance of the as‐obtained electrode can be attributed to the strong electronic interactions between different metal atoms, abundant amorphous/crystalline hetero‐interfaces, and 3D porous nickel mesh structure. Finally, we coupled NiFeMoS/NM as both the anode and cathode in the anion exchange membrane electrolyzer, which can achieve low cell voltage and high stability at ampere‐level current density, demonstrating the great potential of practicability.