bluuebunny/crossref_metadata_2025_split huggingface dataset:
New role of gramicidin A in RIG‐I‐like receptors‐mediated IFN signalling
Authors: Li Xiao , Sun Xinyuan , Yan Zhongyi , Zhao Zhenxiang , Pang Zhaojun , Yang Han , Ji Xiaoxin , Lei Yi , Zhu Zixiang , Guo Xiangqian , Mu Xin 
Published: January 2023

AbstractThe pattern recognition receptors (PRRs) sense exogenous molecular patterns most commonly derived from invading pathogens, to active the interferon (IFN) signalling. In the cytoplasm, the viral double‐stranded RNAs (dsRNAs) are sensed by retinoic acid‐inducible gene I (RIG‐I) or melanoma differentiation‐associated protein 5 (MDA5), depending on the length and chemical properties. Through the binding and oligomerizing onto the RNAs, they form filament to initiate the signalling cascade. Regulation of these receptors' activities are essential for manipulating the strength of IFN signalling. Here, through the virtual screening of chemical reagents using the published MDA5‐dsRNA complex structure (PDB: 4GL2), we identified an antibiotic, gramicidin A as a stimulator that enhanced MDA5‐mediated IFN signalling. Cytotoxic assay and IFN signalling assay suggested that disruption of lipid membrane, which is a well‐defined mechanism of gramicidin A to perform its action, was dispensable in this process. Sucrose gradient ultracentrifugation assay showed that the gramicidin A treatment enhanced MDA5 oligomerization status in the presence of dsRNA. Our work implicated a new role of gramicidin A in innate immunity and presented a new tool to manipulate MDA5 activity.
Responses of the photosynthetic characteristics of summer maize to shading stress
Authors: Sun Zhi‐Chao , Geng Wen‐Jie , Ren Bai‐Zhao , Zhao Bin , Liu Peng , Zhang Ji‐Wang 
Published: January 2023

AbstractThe vast majority of the energy and substance required for maize growth and development and related metabolic processes come from the photosynthesis of leaves. Over the past 60 years, the amount of solar radiation reaching the earth's surface has decreased significantly, reducing the photosynthetic rate of crops and leading to a significant reduction in yields. However, (1) the effects of shading stress on stomatal characteristics, electron transport efficiency of summer maize leaves and (2) the adaptability of summer maize to low light are still unclear. In order to investigate this problems, maize hybrid Denghai605 (DH605) was used as the experimental materials, and two treatments of shading (shading degree is 60%) and CK (natural light as control) were set in the field. The results showed that shading stress resulted in chloroplast dysplasia, and the overall performance of the two photosystems was reduced by 27.85%. The number of stomata per unit area decreased by 15.6% and the proportion of stomatal opening decreased by 73.1% after shading, which resulted in a significant 27.7% reduction in intercellular CO2 concentration. Shading significantly reduced photosynthetic rate by affecting stomatal characteristics and electron transport, and the former was the main influencing factor. The decrease in carbon assimilation capacity resulted in insufficient assimilate supply and significantly reduced grain allocation ratio, resulting in a significant yield reduction of 57.5%. However, in the low‐light environment, the expression of chlorophyll‐binding protein in the leaf of summer maize was upregulated, the content of Lhcb1 protein of the light harvesting pigment protein complex II (LHCII) was increased, and the content of photosynthetic pigment, especially chlorophyll b, was increased, which increased the absorption and capture of blue light, improved the efficiency of light energy utilization, and was a stress manifestation of summer maize adapting to shading stress.
Effect of silver‐containing hydrofiber dressing on burn wound healing: A meta‐analysis and systematic review
Authors: Wu Juan‐juan , Zhang Fan , Liu Jing , Yao Hui‐jun , Wang Yan 
Published: January 2023

AbstractBackgroundSilver sulfadiazine is commonly used to treat local burn wounds. Aquacel‐Ag is a hydrogen fiber dressing containing ionic silver that reduces burn wound infection and promotes antimicrobial activity. It is necessary to compare the efficacy of the two in the healing of burns.AimsThe aim of this study was to systematically evaluate the effect of Aquacel‐Ag on burn wound healing.MethodsA computerized search of CNKI, VIP, Wanfang, SinoMed, PubMed, Cochrane Library, EMbase, Science Direct, Web of Science, Wiley Online Library, and Open Access Library databases was performed from January 1, 2000 to December 31, 2021 for randomized controlled clinical trials. The trials on Aquacel‐Ag dressing and silver sulfadiazine in the treatment of burns were selected. Meta‐analysis was performed using Review Manager 5.0 software.ResultsEleven articles were finally included, with 794 burn patients. Meta‐analysis results showed that compared with patients treated with silver sulfadiazine, burn patients treated with Aquacel‐Ag dressing had shorter wound healing time [MD = −2.49, 95% CI (−5.64–0.65), p = 0.12], significantly lower tumor necrosis factor‐α (TNF‐α) level [MD = −0.52, 95% CI (−0.82–0.22), p = 0.0008], higher wound healing rate [MD = 8.41, 95% CI (3.39–13.43), p = 0.001], fewer dressing changes [MD = −3.27, 95% CI (−4.90–1.63), p < 0.0001].ConclusionAquacel‐Ag dressing can shorten wound healing time and effectively reduce inflammatory reactions in burn patients compared with silver sulfadiazine, but their safety still needs further exploration and analysis.
DELLA proteins regulate spore germination and reproductive development in Physcomitrium patens
Authors: Phokas Alexandros , Meyberg Rabea , Briones‐Moreno Asier , Hernandez‐Garcia Jorge , Wadsworth Panida T. , Vesty Eleanor F. , Blazquez Miguel A. , Rensing Stefan A. , Coates Juliet C. 
Published: January 2023

Summary

Proteins of the DELLA family integrate environmental signals to regulate growth and development throughout the plant kingdom. Plants expressing non‐degradable DELLA proteins underpinned the development of high‐yielding ‘Green Revolution’ dwarf crop varieties in the 1960s. In vascular plants, DELLAs are regulated by gibberellins, diterpenoid plant hormones. How DELLA protein function has changed during land plant evolution is not fully understood.

We have examined the function and interactions of DELLA proteins in the moss Physcomitrium (Physcomitrella) patens, in the sister group of vascular plants (Bryophytes).

PpDELLAs do not undergo the same regulation as flowering plant DELLAs. PpDELLAs are not degraded by diterpenes, do not interact with GID1 gibberellin receptor proteins and do not participate in responses to abiotic stress. PpDELLAs do share a function with vascular plant DELLAs during reproductive development. PpDELLAs also regulate spore germination. PpDELLAs interact with moss‐specific photoreceptors although a function for PpDELLAs in light responses was not detected. PpDELLAs likely act as ‘hubs’ for transcriptional regulation similarly to their homologues across the plant kingdom.

Taken together, these data demonstrate that PpDELLA proteins share some biological functions with DELLAs in flowering plants, but other DELLA functions and regulation evolved independently in both plant lineages.
Reconstitution and expression of mcy gene cluster in the model cyanobacterium Synechococcus 7942 reveals a role of MC‐LR in cell division
Authors: Zheng Yanli , Xue Chunling , Chen Hui , Jia Anqi , Zhao Liang , Zhang Junli , Zhang Lixin , Wang Qiang 
Published: January 2023

Summary
Cyanobacterial blooms pose a serious threat to public health due to the presence of cyanotoxins. Microcystin‐LR (MC‐LR) produced by Microcystis aeruginosa is the most common cyanotoxins. Due to the limitation of isolation, purification, and genetic manipulation techniques, it is difficult to study and verify in situ the biosynthetic pathways and molecular mechanisms of MC‐LR.
We reassembled the biosynthetic gene cluster (mcy cluster) of MC‐LR in vitro by synthetic biology, designed and constructed the strong bidirectional promoter biPpsbA2, transformed it into Synechococcus 7942, and successfully expressed MC‐LR at a level of 0.006–0.018 fg cell−1 d−1.
We found the expression of MC‐LR led to abnormal cell division and cellular filamentation, further using various methods proved that by irreversibly competing its GTP‐binding site, MC‐LR inhibits assembly of the cell division protein FtsZ.
The study represents the first reconstitution and expression of the mcy cluster and the autotrophic production of MC‐LR in model cyanobacterium, which lays the foundation for resolving the microcystins biosynthesis pathway. The discovered role of MC‐LR in cell division reveals a mechanism of how blooming cyanobacteria gain a competitive edge over their nonblooming counterparts.
Real‐world trends in atrial fibrillation ablation indicate increasing durability of pulmonary vein isolation at repeat ablation
Authors: Morales Gustavo , Hunter Tina D. , Rajendra Anil , Boo Lee Ming , Osorio Jose 
Published: January 2023

AbstractBackgroundDurable electrical isolation of pulmonary veins (PVs) is associated with better outcomes after atrial fibrillation (AF) ablation, but previous studies of AF recurrence have reported high rates of reconnection despite successful acute isolation. This study aims to quantify historical trends in the durability of PV isolation (PVI) as radiofrequency (RF) ablation catheters, additional ablation technologies, and associated workflows have evolved.MethodsThe study population included adult patients receiving a first repeat ablation for AF between September 2013 and July 2019 at the study site. All index ablations were performed at the same site with an RF catheter and included PVI. Three generations of irrigated RF catheters based on the same technology platform were used by the site during the timeframe of this study.ResultsA total of 224 patients were included in the analysis. At repeat ablation, the mean number of patients with at least one reconnected PV dropped significantly with subsequent catheter generation, from 78.3% to 56.7% to 27.0% (p < .0001). Moreover, the mean number of reconnected PVs were significantly reduced from 1.48 to 0.92 to 0.47 (p < .0001), representing a 68.3% reduction across the 3 generations of devices.ConclusionSignificant improvement in durable PVI was seen with successive generations of RF catheter over a 6‐year period. In addition to catheter technology, ancillary advances in ablation technologies, workflows, and operator experience likely contributed to these improvements.
A near‐complete genome assembly ofBrassica rapaprovides new insights into the evolution of centromeres
Authors: Zhang Lei , Liang Jianli , Chen Haixu , Zhang Zhicheng , Wu Jian , Wang Xiaowu 
Published: January 2023

SummaryBrassica rapacomprises many important cultivated vegetables and oil crops. However, Chiifu v3.0, the currentB. rapareference genome, still contains hundreds of gaps. Here, we presented a near‐complete genome assembly ofB. rapaChiifu v4.0, which was 424.59 Mb with only two gaps, using Oxford Nanopore Technology (ONT) ultralong‐read sequencing and Hi‐C technologies. The new assembly contains 12 contigs, with a contig N50 of 38.26 Mb. Eight of the ten chromosomes were entirely reconstructed in a single contig from telomere to telomere. We found that the centromeres were mainly invaded byALEandCRMlong terminal repeats (LTRs). Moreover, there is a high divergence of centromere length and sequence amongB. rapagenomes. We further found that centromeres are enriched forCopiainvaded at 0.14 MYA on average, while pericentromeres are enriched forGypsyLTRs invaded at 0.51 MYA on average. These results indicated the different invasion mechanisms of LTRs between the two structures. In addition, a novel repetitive sequencePCR630was identified in the pericentromeres ofB. rapa. Overall, the near‐complete genome assembly,B. rapaChiifu v4.0, offers valuable tools for genomic and genetic studies ofBrassicaspecies and provides new insights into the evolution of centromeres.
The secrets that you keep: Secrets and relationship quality
Authors: Davis Christopher G. , Tabri Nassim 
Published: January 2023

AbstractResearch indicates that preoccupation with secrets takes a toll on mental health. There is also some evidence to suggest that keeping secrets from one's romantic partner may harm the relationship as it implies a lack of trust that one's partner will be supportive. Two 4‐wave longitudinal surveys of 609 adults (34.3% men, 65.4% women; Mage = 36.4) keeping a secret from their romantic partner was used to assess the lagged effect of preoccupation with the secret on subsequent relationship satisfaction, trust, and emotional intimacy as well as the reverse. Cross‐lagged panel modeling with random intercepts indicated that whereas individuals in poorer relationships were more preoccupied with their secrets, there was no evidence for cross‐lagged effects. The findings suggest that secrecy may be a symptom—not a cause—of a dissatisfying, distrustful and distant relationship.
Public health nursing workforce and learning needs: A national sample survey analysis
Authors: Castner Jessica , Stanislo Kimberly , Castner Martin , Monsen Karen A. 
Published: January 2023

AbstractObjectivesGenerate national estimates of the public health nursing workforce's (1) demographic and work characteristics and (2) continuing education learning needs in the United States.DesignSecondary data analysis of the 2018 National Sample Survey of Registered Nurses.SampleTotal 7352 of the 50,273 survey respondents were categorized as public health nurses (PHNs), representing an estimated 467,271 national workforce.MeasurementsSurvey items for demographics, practice setting, training topics, and language(s) spoken fluently were analyzed.ResultsWorkforce demographic characteristics are included. Mental health training was the most frequently endorsed topic by PHNs, followed by patient‐centered care and evidence‐based care. Training topic needs vary by practice setting.ConclusionsResults here can be used as a needs assessment for national public health nursing professional development and education initiatives. Further research is needed to refine and survey a nationally representative sample in a manner meaningful to public health nursing practice.
Physiology and proteomics analyses reveal the response mechanisms of Rhizophora mucronata seedlings to prolonged complete submergence
Authors: Piro A. , Mazzuca S. , Phandee S. , Jenke M. , Buapet P. 
Published: January 2023

Abstract

Mangrove seedlings are subject to natural tidal inundation, while occasional flooding may lead to complete submergence. Complete submergence reduces light availability and limits gas exchange, affecting several plant metabolic processes. The present study focuses on Rhizophora mucronata, a common mangrove species found along the coasts of Thailand and the Malay Peninsula.

To reveal response mechanisms of R. mucronata seedlings to submergence, a physiological investigation coupled with proteomic analyses of leaf and root tissues was carried out in plants subjected to 20 days of control (drained) or submerged conditions.

Submerged seedlings showed decreased photosynthetic activity, lower stomatal conductance, higher total antioxidant capacity in leaves and higher lipid peroxidation in roots than control plants. At the same time, tissue nutrient ion content displayed organ‐specific responses. Proteome analysis revealed a significant change in 240 proteins in the leaves and 212 proteins in the roots. In leaves, most differentially accumulated proteins (DAPs) are associated with nucleic acids, stress response, protein transport, signal transduction, development and photosynthesis. In roots, most DAPs are associated with protein metabolic process, response to abiotic stimulus, nucleic acid metabolism and transport.

Our study provides a comprehensive understanding of submergence responses in R. mucronata seedlings. The results suggest that submergence induced multifaceted stresses related to light limitation, oxidative stress and osmotic stress, but the responses are organ specific. The results revealed many candidate proteins which may be essential for survival of R. mucronata under prolonged submergence.
Microbiome and metabolome reveal the metabolic and microbial variations induced by depression and constipation
Authors: Liu Xiaojie , Zhao Ziyu , Fan Yuhui , Zhao Di , Wang Yaze , Lv Meng , Qin Xuemei 
Published: January 2023

BackgroundDepressed patients are often accompanied with constipation symptoms, and vice versa. However, the underlying mechanisms of such a bidirectional correlation have remained elusive. We aim to reveal the possible correlations between depression and constipation from the perspectives of gut microbiome and plasma metabolome.MethodsWe constructed the depressed model and the constipated model of rats, respectively. First, we measured the locomotor activity status and the gastrointestinal functions of rats. And then, nuclear magnetic resonance plasma metabolomics was applied to reveal the shared and the unique metabolites of depression and constipation. In addition, 16 S ribosomal RNA gene sequencing was used to detect the impacts of constipation and depression on gut microbiota of rats. Finally, a multiscale and multifactorial network, that is, the ‘phenotypes ‐ differential metabolites ‐ microbial biomarkers’ integrated network, was constructed to visualise the mechanisms of connections between depression and constipation.ResultsWe found that spontaneous locomotor activity and gastrointestinal functions of both depressed rats and constipated rats significantly decreased. Further, eight metabolites and 14 metabolites were associated depression and constipation, respectively. Among them, seven metabolites and four metabolic pathways were shared by constipation and depression, mainly perturbing energy metabolism and amino acid metabolism. Additionally, depression and constipation significantly disordered the functions and the compositions of gut microbiota of rats, and decreased the ratio of Firmicutes to Bacteroidetes.ConclusionThe current findings provide multiscale and multifactorial perspectives for understanding the correlations between depression and constipation, and demonstrate new mechanisms of comorbidity of depression and constipation.
Ethnography, Incongruity, History: Soviet Poetic Cinema
Authors: Papazian Elizabeth A. 
Published: January 2023

AbstractThis essay examines the entangling of the poetic and the ethnographic in the art cinema of the 1960s as an indicator of a broader collision of epistemological/discursive regimes in postwar Soviet cinema—and ultimately, a clash between two fundamentally opposed approaches to the discursive production of history. In the Soviet poetic cinema of the 1960s, the temporal‐spatial frameworks of the Stalin era are disrupted, shifting first of all, to what Tarkovsky called a lived experience of time—that is, to the subjective emotions and experiences of individual people; second, to localized histories that may not coincide with the supra‐national Soviet developmental narrative; and third, to the positing of an archaic, even pre‐historical temporality as a kind of lost ideal. I argue that poetic cinema serves as a site for playing out the contradiction among temporalities and spatialities in post‐Stalin culture, and therefore among opposed sense‐making projects and representational modes, creating the possibility for subverting the colonial function of Soviet cinema.
Remove unwanted variation retrieves unknown experimental designs
Authors: Lönnstedt Ingrid M. , Speed Terence P. 
Published: January 2023

AbstractRemove unwanted variation (RUV) is an estimation and normalization system in which the underlying correlation structure of a multivariate dataset is estimated from negative control measurements, typically gene expression values, which are assumed to stay constant across experimental conditions. In this paper we derive the weight matrix which is estimated and incorporated into the generalized least squares estimates of RUV‐inverse, and show that this weight matrix estimates the average covariance matrix across negative control measurements. RUV‐inverse can thus be viewed as an estimation method adjusting for an unknown experimental design. We show that for a balanced incomplete block design (BIBD), RUV‐inverse recovers intra‐ and interblock estimates of the relevant parameters and combines them as a weighted sum just like the best linear unbiased estimator (BLUE), except that the weights are globally estimated from the negative control measurements instead of being individually optimized to each measurement as in the classical, single measurement BIBD BLUE.
Disrupted interoception in Military Service Members and Veterans with a history of suicidality
Authors: Smith April R. , Witte Tracy K. , Grunewald William , Kinkel‐Ram Shruti , Santivasci Celeste , Crosby Eric , Williams Tammy , Esche Aaron , Tubman David , Dretsch Michael 
Published: January 2023

AbstractIntroductionThis project tested whether Service Members (SM) and Veterans with current suicidal ideation or a history of suicide attempt had greater interoceptive dysfunction than SM and Veterans with past or no suicidal ideation.MethodParticipants (N = 195; 69% male) were SM (62%) and Veterans (38%) who completed measures of suicidal thoughts and behaviors and subjective and objective interoceptive dysfunction. Participants were split into the following suicide groups: no suicidality, lifetime ideation, current ideation, and past attempt. Planned orthogonal contrasts tested for differences.ResultsThe combined suicidality group (lifetime ideation, current ideation, or past attempt) had worse body trust relative to the no suicidality group, and the current ideation group had worse body trust relative to those with lifetime ideation. Those with a history of suicide attempt had worse body appreciation than the combined group of ideators, and those with current ideation had worse body appreciation relative to those with lifetime ideation. The groups did not differ on objective interoception.ConclusionInteroception is disrupted among individuals with suicidality histories within a predominantly male‐identified military sample. Individuals with current suicidal ideation had both worse body trust and appreciation relative to those with past ideation. Suicide risk assessments may benefit from including questions related to body trust.
Simulating urban land‐use change based on the multi‐hierarchal planning–environment interaction process
Authors: Yang Jing , Sun Yizhong , Zhu Jie , Song Shuying 
Published: January 2023

AbstractUrban land‐use change is the result of coupling interaction between planning and environment systems. The aim of our study was to construct an effective model to show how the urban land‐use changes under the planning–environment interaction system with multi‐hierarchy and major function oriented zoning. Combining the Cellular automata (CA) model with logistic regression model, the proposed multi‐hierarchal vector CA model (MH‐VCA3) was constructed by mining multi‐hierarchal land‐use transition rules under the planning–environment interaction system. Taking Jiangyin City (China) as an example, we compared the simulated result of the proposed model to those of the well‐accepted Logistic CA and traditional multi‐level CA models to demonstrate the effectiveness of the consideration of top‐down decomposition constraint and bottom‐up updating. Furthermore, by simulating the land‐use changes under different population regionalization scenarios, we found that in order to form the spatial pattern of “agglomeration in the north and ecology in the south,” the planned population growth at the global hierarchal level should be allocated to the district units according to the law of Central district > Chengxi district > Chengdong district > Chengnan district > Chengdongnan district. The proposed model is expected to provide scientific support for the formulation of urban planning schemes in the future.