bluuebunny/crossref_metadata_2025_split huggingface dataset:
The submandibular and sublingual glands maintain oral microbial homeostasis through multiple antimicrobial proteins
Authors: Li Yanan , Liu Jingming , Guan Tong , Zhang Yuxin , Cheng Qianyu , Liu Huikai , Liu Chang , Luo Wenping , Chen Hong , Chen Liang , Zhao Tianyu 
Published: January 2023

IntroductionOral microbial homeostasis is a key factor affecting oral health, and saliva plays a significant role in maintaining oral microbial homeostasis. The submandibular gland (SMG) and sublingual gland (SLG) together produce the most saliva at rest. Organic ingredients, including antimicrobial proteins, are rich and distinctive and depend on the type of acinar cells in the SMG and SLG. However, the functions of the SMG and SLG in maintaining oral microbial homeostasis have been difficult to identify and distinguish, given their unique anatomical structuresMethodsIn this study, we independently removed either the SMG or SLG from mouse models. SMGs were aseptically removed in three mice in the SMG-removal group, and SLGs were aseptically removed in three mice in the SLG-removal group. Three mice from the sham-operated group were only anesthetized and incised the skin. After one month, we analyzed their oral microbiome through 16S rRNA sequencing. And then, we analyzed each gland using proteomics and single-cell RNA sequencing.ResultsOur study revealed that the microbiome balance was significantly disturbed, with decreased bacterial richness, diversity, and uniformity in the groups with the SMG or SLG removed compared with the sham-operated group. We identified eight secreted proteins in the SMG and two in the SLG that could be involved in maintaining oral microbial homeostasis. Finally, we identified multiple types of cells in the SMG and SLG (including serous acinar, mucinous acinar, ductal epithelial, mesenchymal, and immune cells) that express potential microbiota homeostasis regulatory proteins. Our results suggest that both the SMG and SLG play crucial roles in maintaining oral microbial homeostasis via excretion. Furthermore, the contribution of the SMG in maintaining oral microbial homeostasis appears to be superior to that of the SLG. These findings also revealed the possible antimicrobial function of gland secreta.DiscussionOur results suggest that control of oral microbial dysbiosis is necessary when the secretory function of the SMG or SLG is impaired. Our study could be the basis for further research on the prevention of oral diseases caused by microbial dysbiosis.
Changes in the microbiota in different intestinal segments of mice with sepsis
Authors: Peng Yahui , Wei Jieling , Jia Xiaonan , Luan Feiyu , Man Mingyin , Ma Xiaohui , Luo Yinghao , Li Yue , Li Nana , Wang Qian , Wang Xibo , Zhou Yang , Ji Yuanyuan , Mu Wenjing , Wang Jun , Wang Chunying , Zhang Qianqian , Yu Kaijiang , Zhao Mingyan , Wang Changsong 
Published: January 2023

IntroductionThe small intestine, as the main digestion and absorption site of the gastrointestinal tract, is often overlooked in studies, and the overall microbiota does not reflect the makeup of the microbiota in different segments of the intestine. Therefore, we aimed to exclude the influence of routine ICU treatment measures on sepsis patients and observed changes in the diversity and abundance of gut microbiota in different intestinal segments of septic mice.MethodsThe mice were randomly divided into the CLP6h group and the sham group. The contents of the colon and small intestine of the experimental group and the control group were collected after 6 h.ResultsAfter CLP, the number and structure of the gut microbiota in the colon changed most obviously, among which Bacteroidetes had the most significant changes. Akkermansia, D.Firmicutes_bacterium_M10_2, Blautia, Bifidobacterium, Lactobacillus, Candidatus_Arthromitus, and Muribaculaceae were changed in the colon. Lactobacillus, Bifidobacterium, Akkermansia, Blautia, Candidatus_Arthromitus, and Lachnospiraceae_NK4A136_group were changed in the small intestine.DiscussionOur experiment found that there were different numbers of unique and common gut microbiota in the small intestine and colon after sepsis, and the gut microbiota of the colon changed more drastically after sepsis than the small intestine. Thus, we should focus on protective gut microbiota and mucin-degrading microbes. We hope that these results will provide help for sepsis treatment in the future.
Rhine low water crisis: From individual adaptation possibilities to strategical pathways
Authors: Gobert Julie , Rudolf Florence 
Published: January 2023

IntroductionIn 2018, the Rhine transport sector experienced an unprecedented low water crisis, during which large cargo vessels were no longer able to navigate on certain sections of the river. This led to a major disruption in inland waterway transport. This article aims at questioning how the crisis acted as a stimulus for port authorities and their customers to consider the risks for their assets and operations and as a window of opportunity for creating a new collective and for defining “solutions.”MethodologyInspired by the Impact Chain methodology, a step-by-step protocol integrating focus groups and interviews, was applied so that stakeholders affected by low waters can identify their individual and common vulnerability and define possible ways of acting (pathways).ResultsOne of these pathways, the transitional infrastructural pathway, targets to increase the water level and overcome low water levels (use of Lake Constance as a water reservoir or creation of new water storage areas; deepening of the channel at Kaub and Maxau). It appears as the most suitable because it is a technical, well-controlled process that provides a comfortable solution in the short term. It exemplifies the lock-ins set by infrastructure.DiscussionHowever, the participative approach also highlights the fundamental challenge of developing new processes and new intermodal organizations in the long term.
Modified Linggui Zhugan Decoction protects against ventricular remodeling through ameliorating mitochondrial damage in post-myocardial infarction rats
Authors: Xiang Mi , Zhao Xin , Lu Yingdong , Zhang Yang , Ding Fan , Lv Lifei , Wang Yuling , Shen Zihuan , Li Li , Cui Xiangning 
Published: January 2023

IntroductionModified Linggui Zhugan Decoction (MLZD) is a Traditional Chinese Medicine prescription developed from Linggui Zhugan Decoction (LZD) that has been used for the clinical treatment of ischemic cardiovascular diseases. However, the cardioprotective mechanism of MLZD against post-myocardial infarction (MI) ventricular remodeling remains unclear.MethodsWe explored the effects of MLZD on ventricular remodeling and their underlying mechanisms, respectively, in SD rats with MI models and in H9c2 cardiomyocytes with oxygen-glucose deprivation (OGD) models. The cardiac structure and function of rats were measured by echocardiography, HE staining, and Masson staining. Apoptosis, inflammation, mitochondrial structure and function, and sirtuin 3 (SIRT3) expression were additionally examined.ResultsMLZD treatment significantly ameliorated cardiac structure and function, and thus reversed ventricular remodeling, compared with the control. Further research showed that MLZD ameliorated mitochondrial structural disruption, protected against mitochondrial dynamics disorder, restored impaired mitochondrial function, inhibited inflammation, and thus inhibited apoptosis. Moreover, the decreased expression level of SIRT3 was enhanced after MLZD treatment. The protective effects of MLZD on SIRT3 and mitochondria, nevertheless, were blocked by 3-TYP, a selective inhibitor of SIRT3.DiscussionThese findings together revealed that MLZD could improve the ventricular remodeling of MI rats by ameliorating mitochondrial damage and its associated apoptosis, which might exert protective effects by targeting SIRT3.
Involvement of SUR2/Kir6.1 channel in the physiopathology of pulmonary arterial hypertension
Authors: Le Ribeuz Hélène , Masson Bastien , Dutheil Mary , Boët Angèle , Beauvais Antoine , Sabourin Jessica , De Montpreville Vincent Thomas , Capuano Véronique , Mercier Olaf , Humbert Marc , Montani David , Antigny Fabrice 
Published: January 2023

AimsWe hypothesized that the ATP-sensitive K+ channels (KATP) regulatory subunit (ABCC9) contributes to PAH pathogenesis. ABCC9 gene encodes for two regulatory subunits of KATP channels: the SUR2A and SUR2B proteins. In the KATP channel, the SUR2 subunits are associated with the K+ channel Kir6.1. We investigated how the SUR2/Kir6.1 channel contributes to PAH pathogenesis and its potential as a therapeutic target in PAH.Methods and resultsUsing in vitro, ex vivo, and in vivo approaches, we analyzed the localization and expression of SUR2A, SUR2B, and Kir6.1 in the pulmonary vasculature of controls and patients with PAH as in experimental pulmonary hypertension (PH) rat models and its contribution to PAH physiopathology. Finally, we deciphered the consequences of in vivo activation of SUR2/Kir6.1 in the monocrotaline (MCT)-induced PH model. We found that SUR2A, SUR2B, and Kir6.1 were expressed in the lungs of controls and patients with PAH and MCT-induced PH rat models. Organ bath studies showed that SUR2 activation by pinacidil induced relaxation of pulmonary arterial in rats and humans. In vitro experiments on human pulmonary arterial smooth muscle cells and endothelial cells (hPASMCs and hPAECs) in controls and PAH patients showed decreased cell proliferation and migration after SUR2 activation. We demonstrated that SUR2 activation in rat right ventricular (RV) cardiomyocytes reduced RV action potential duration by patch-clamp. Chronic pinacidil administration in control rats increased heart rate without changes in hemodynamic parameters. Finally, in vivo pharmacological activation of SUR2 on MCT and Chronic-hypoxia (CH)-induced-PH rats showed improved PH.ConclusionWe showed that SUR2A, SUR2B, and Kir6.1 are presented in hPASMCs and hPAECs of controls and PAH patients. In vivo SUR2 activation reduced the MCT-induced and CH-induced PH phenotype, suggesting that SUR2 activation should be considered for treating PAH.
Effectiveness of Yiqi Fumai lyophilized injection for acute heart failure: Rationale and design of the AUGUST-AHF cohort study
Authors: Zhang Xuecheng , Kang Jing , Zhang Jingjing , Chen Ying , Dai Hengheng , Hu Mingzhi , Liu Yan , Shang Hongcai 
Published: January 2023

IntroductionThe effect of Yiqi Fumai lyophilized injection (YQFM) on acute heart failure (AHF) patients has been evaluated in a large sample, randomized, controlled trial (AUGUST-AHF RCT study). However, restrictive eligibility criteria from a randomized clinical trial may raise concerns about the generalizability of the results to under-represented groups or complex patients with multimorbidity. Therefore, we intend to conduct the AUGUST-AHF cohort study which aims to assess the effectiveness of YQFM in patients with AHF in a real-world setting and compare the results with AUGUST-AHF RCT study.Methods and analysisThis prospective, multicenter cohort study will be conducted at 50 secondary and tertiary hospitals in China and comprise 1,200 patients with AHF. The participants will be followed for up to at least 180 days. The primary outcome is a composite of 90-day all-cause mortality or readmission for heart failure. The secondary outcomes include length of hospital stay, cardiac-specific death, MACE, NYHA cardiac function classification. Cox proportional-hazards regression models will be used to estimate the association between YQFM use and the primary outcome. The primary analysis will use propensity-score matching methods to balance the differences in baseline variables between treatment cohorts.Ethics and disseminationApproval for the study has been obtained from the Ethical Committee of Dongzhimen Hospital (approval No. 2022DZMEC-327-02) and registered at ClinicalTrials.gov (NCT05586048). The study results will be published in peer-reviewed journals and presented at scientific conferences.
Evaluating western North Pacific tropical cyclone forecast in the subseasonal to seasonal prediction project database
Authors: Wang Xiaochun , Waliser Duane , Jiang Xianan , Asharaf Shakeel , Vitart Frederic , Jie Weihua 
Published: January 2023

The Daily Tropical Cyclone Probability (DTCP), defined as the probability of tropical cyclone occurrence within 500 km of a location in 1 day, is proposed and used in evaluating subseasonal to seasonal (S2S) predictions from the subseasonal to seasonal Prediction Project Database, from May 1 to October 31, 1999 to 2010. The ensemble forecasts are collected from eleven operational centers, the Bureau of Meteorology (BoM), China Meteorological Administration (CMA), Environment and Climate Change Canada (ECCC), European Centre for Medium-Range Weather Forecasts (ECMWF), Hydrometeorological Centre of Russia (HMCR), the Institute of Atmospheric Sciences and Climate of the National Research Council of Italy (ISAC), the Japan Meteorological Agency (JMA), the Korea Meteorological Administration (KMA), Météo-France/Centre National de Recherche Meteorologiques (METFR), the United States National Centers for Environmental Prediction (NCEP), and the United Kingdom Met Office (UKMO). In both observation and these eleven forecast models, the daily tropical cyclone probability is modulated by the Boreal Summer Intraseasonal Oscillation (BSISO), depicted by the two indices, boreal summer intraseasonal oscillation 1 and boreal summer intraseasonal oscillation 2. During boreal summer intraseasonal oscillation 1 phases 1, 5, 6, 7, and 8, the daily tropical cyclone probability in the western North Pacific region is ∼3.5 times higher. Similarly, during phases 1, 2, 3, 4, and 8 of boreal summer intraseasonal oscillation 2, the daily tropical cyclone probability is ∼2.5 times higher. Among the eleven models, the European Centre for Medium-Range Weather Forecasts model best reproduces the climatological daily tropical cyclone probability and its modulation by the boreal summer intraseasonal oscillation in the western North Pacific region, followed by the United States National Centers for Environmental Prediction, the Korea Meteorological Administration, the Japan Meteorological Agency models. Using the daily tropical cyclone probability metric, the highest debiased Brier Skill Score of the eleven models is from European Centre for Medium-Range Weather Forecasts, which has a slightly less skillful prediction than the reference climatological forecast with lead time 11–30 days. The skill of the eleven models is higher during the non-active phases of tropical cyclone activity than their skill during the active phases.
Seismic site classification of Nakhon Nayok City, central Thailand, using the MASW technique
Authors: Thitimakorn Thanop , Arjwech Rungroj , Kupongsak Sasikan 
Published: January 2023

Shear-wave velocity (Vs) of soils in Nakhon Nayok City, Central Thailand were determined using the Multi-Channel Analysis of Surface Wave technique. The Vs. of 35 tested sites were weighted average to 30 m depth based on the National Earthquake Hazards Reduction Program (NEHRP) criteria, which revealed NEHRP site classes C, D, and E within the study area. Soil class C was found in the north of the city. Moreover, most of the residential area of Nakhon Nayok City is positioned on soil classes D and E. The soil in the area of study has an amplification value ranging from 1.4 to 3.9. The central and southwestern parts of the city, which mainly consist of soft sediments, have a higher amplification value than that in the northern part, which mainly consists of coarse grain soils of colluvial deposits. Based on the NEHRP and amplification value, the Nakhon Nayok City area will experience some effect from ground shaking from nearby and far away earthquake seismic sources.
In situ observation of wave-induced deformation of submarine landslides in tidal channel areas
Authors: Xue Liang , Liu Hanlu , Li Zhenghui , Sun Zhongqiang , Shan Hongxian , Jia Yonggang 
Published: January 2023

Landslides often occur in land slopes, estuaries, fjords, and intertidal zones in the marine environment, while landslides in tidal channels have not been systematically reported. The Zhoushan sea area is dotted with islands and crisscross tidal channels, which is a typical tidal channel development area. The data from the coastal zone and island survey, offshore engineering site selection, and environmental survey show that landslides are very common in tidal channel areas. The detailed study of this kind of landslide will enrich the research theory of submarine landslide and have important guiding significance for marine planning, development and site selection, and construction of coastal engineering in coastal areas. First, the submarine landslide in southwest of Zhujiajian Island in Zhoushan is investigated in this study. It is found that the sliding zone is parallel to the island coastline, with a length of about 250 m and an extension of 2 km, and the buried depth of the sliding surface is generally 8–10 m; then, through the self-developed seabed deformation observation system, combined with the hydrodynamic observation system, the landslide was observed in situ for 75 days. The observation results showed that the landslide slides at a constant speed along the sliding surface under the action of weight; the slides accelerate under the action of waves, and multiple sliding surfaces are generated in the landslide body at the same time. Finally, the genetic mechanism of submarine landslides in tidal channel areas is put forward.
Hazard assessment and formation mechanism of debris flow outbursts in a small watershed of the Linxia Basin
Authors: Yang Weimin , Wan Feipeng , Ma Siqi , Qu Jingkai , Zhang Chunshan , Tang Haibing 
Published: January 2023

The Linxia Basin is located in the transition zone between the Qinghai–Tibet Plateau and the Loess Plateau in China. Collapse–landslide–debris flow geological disasters are particularly prevalent in this region. Taking a debris flow that occurred in Zhangjiayuan gully, Dongxiang County, as an example, the characteristics and formation mechanism were studied through field investigation and remote sensing interpretation methods. The hazard zoning and influence range of the debris flow were analysed under different precipitation conditions using numerical simulations. The results show that the debris flow in Zhangjiayuan gully experienced medium-to small-scale, rapid and catastrophic viscous mud flows, with activity frequencies ranging from medium to low. Because large numbers of landslides, collapses and overland flows developed on the gully bank slope, material sources for debris flows were abundant but were supplied intermittently. Induced by heavy rainfall, the landslides on the bank slipped into the gully, and then the landslide mass blocked the channel and formed a weir dam. As water converged in the gully, the dam body softened to mud, seepage deformation occurred, and the dam then burst to form a debris flow. This disaster mode represents a kind of rainfall-type landslide–blockage–debris flow disaster chain. Under varying conditions in which heavy rainfall occurs once every 10 years or once every 50 years, debris flows may break out in Zhangjiayuan gully, and the Zheda highway and the associated tunnels located below the gully are in medium–high debris flow danger zones. When a debris flow rushes out of the gully mouth and cannot be discharged in time, it accumulates and buries the highway and tunnels. Therefore, attention should be given to preventing such small-scale sudden debris flow disasters and strengthening the early warning capacity of debris flow prevention to ensure the safe operation of highway.
Comparison of the efficacy of endoscopic submucosal dissection and transanal endoscopic microsurgery in the treatment of rectal neuroendocrine tumors ≤ 2 cm
Authors: Jin Rui , Bai Xiaoyin , Xu Tianming , Wu Xi , Wang Qipu , Li Jingnan 
Published: January 2023

IntroductionCurrently, complete tumor resection is considered the most effective treatment for rectal neuroendocrine tumors (NETs). Endoscopic submucosal dissection (ESD) and transanal endoscopic microsurgery (TEM) are recommended for rectalNETs ≤2 cm, but it is not clear which method is better. Thus, we evaluated the efficacy of ESD and TEM in the treatment of rectal neuroendocrine tumors (NETs) ≤ 2 cm.MethodsWe conducted a single-centre retrospective cohort study between 2010 and 2021 of rectal NETs ≤ 2 cm in 114 patients with long-term follow-up data who were divided into ESD (n=55) and TEM groups (n=59). Our study assessed differences between groups in the complete resection rate of lesions, recurrence rate, surgical complications, procedure time, and length of hospital stay.ResultsThe co-primary outcomes were the complete resection rate of lesions and the recurrence rate. Compared to that in the ESD group, the complete resection rate was significantly higher in the TEM group (91.5% vs. 70.9%, p=0.005). The median follow-up time was 22 months in our study, and the follow-up outcomes suggested that the rates of recurrence were 1.8% (1/55) and 6.8% (4/59) in the ESD and TEM groups, respectively, with no significant difference between the two groups. The secondary outcomes of the evaluation were surgical complications, procedural time, and length of hospital stay. The rate of complications (gastrointestinal bleeding and perforation) was low in both the ESD (7.3%, 4/55) and TEM (5.1%, 3/59) groups. No difference in hospitalization duration was observed between the two groups in our study. However, the procedure time was significantly shorter in the ESD group than in the TEM group (27.5 min vs. 56 min, p&lt;0.001).ConclusionsAlthough the rate of complete resection in the TEM group was higher than that in the ESD group, there was no difference in recurrence rates between the two modalities during long-term follow-up. Depending on the qualities of the available hospital resources in the area, one of the two approaches can be adopted.
Screening for non-classic congenital adrenal hyperplasia in women: New insights using different immunoassays
Authors: Nakhleh Afif , Saiegh Leonard , Shehadeh Naim , Weintrob Naomi , Sheikh-Ahmad Mohammad , Supino-Rosin Lia , Alboim Sandra , Gendelman Raya , Zloczower Moshe 
Published: January 2023

ContextThe 250µg-cosyntropin stimulation test (CST) is used to diagnose non-classic congenital adrenal hyperplasia (NCCAH). The current recommendation is to perform CST when follicular 17-hydroxyprogesterone (17OHP) is 6-30 nmol/L, a cutoff derived from radioimmunoassay (RIA). Recently, enzyme-linked immunosorbent assay (ELISA) has replaced RIA.ObjectivesWe aimed to (1) determine the RIA and ELISA-based 17OHP cutoffs at which CST should be performed, (2) identify predictors of NCCAH.MethodsA retrospective study at an Israeli Health Maintenance Organization. Data were retrieved from women with suspected NCCAH, referred for CST during 2001–2020. NCCAH was defined as a stimulated 17OHP &gt;30 nmol/L. Serum 17OHP levels were assayed by RIA from 1/2000-3/2015, and by ELISA from 4/2015-12/2020. ROC curves were generated and optimal 17OHP thresholds were determined. Multivariate analysis was performed.ResultsCST was performed in 2409 women (1564 in RIA, 845 in ELISA). NCCAH was diagnosed in 4.7% of the RIA group and 7.5% of the ELISA group. The optimal basal 17OHP cutoff values predicting NCCAH were 6.1 nmol/L in RIA (sensitivity=93.2%, specificity=91.7%) and 8.2 nmol/L in ELISA (sensitivity=93.7%, specificity=92.3%). In multivariate analysis, higher basal 17OHP, lower LH: FSH ratio, and oligomenorrhea were predictors of NCCAH in RIA. Higher basal 17OHP, androstenedione, and total testosterone were predictors of NCCAH in ELISA. A lower LH: FSH ratio showed similar trend in ELISA.ConclusionsOptimal RIA-based basal 17OHP cutoff was comparable with that recommended in guidelines. The results suggest adopting a higher 17OHP cutoff when using ELISA. LH : FSH ratio improves the negative predictive value of basal 17OHP.