DOP041 The association between fermentable dietary fibre intake and risk of developing Crohn’s Disease: Results from the GEM Project
Authors: McShane C , Xue M , Kim J , Leibovitzh H , Shao J , Li Q , Madsen K , Khorasaniha R , Griffiths A M , Walters T , Steinhart H , Silverberg M , Dieleman L A , Huynh H Q , Panacionne R , Beck P , Aumais G , Wrobel I , Bitton A , Marshall J , Bernstein C N , Hyams J , Moayyedi P , Lee S H , Armstrong H , Croitoru K , Turpin W ,   The Crohn’s and Colitis Canada GEM Project Research Consortium full list of affiliations available at www.gemproject.ca
Published: January 2025

Abstract

Background
The cause of Crohn’s Disease (CD) remains unclear; however evidence suggests that dietary factors play a key role. With a rising incidence and no definitive cure, identifying modifiable risk factors is critical in preventing CD development. We aimed to study the association between fermentable dietary fibre intake and the future development of CD in an at-risk population.

Methods
Participants were recruited as part of the Crohn’s and Colitis Canada GEM study, a prospective cohort study of healthy first-degree relatives of patients with CD. At enrolment, a validated food frequency questionnaire was administered. Pectin, β-glucan, inulin, fructooligosaccharides (FOS), and arabinoxylan intake were quantified, and values energy-adjusted. Survival analysis was used to test the association between intake of fibre subtypes and risk of CD development. Generalized estimating equation tested the association between fibre intake and baseline impaired intestinal permeability (fractional urinary excretion of lactulose to mannitol ratio (LMR) &gt;0.025); subclinical gut inflammation (faecal calprotectin (FCP) &gt;250 μg/g); and faecal microbiome composition via 16s rRNA sequencing.

Results
76 of the 2,659 participants developed CD with a median follow-up of 8.9 years (IQR=5.7-12.3). Median age at recruitment was 17 years (IQR=12-25). 47% were male. Higher intake of inulin (HR = 0.78; 95% CI=0.61–0.99; p=0.04) and β-glucan (HR = 0.78; 95% CI=0.61–0.99; p=0.04) were associated with a reduced risk of CD development. No fibre subtype was associated with a higher risk of CD development. Lower intake of pectin, inulin, FOS and β-glucan was associated with impaired intestinal permeability (0.005&lt;p&lt;0.04). Lower inulin intake was associated with high FCP levels (p = 0.046). Higher pectin intake was associated with increased alpha diversity of faecal microbiome (p = 0.028). Higher inulin, FOS and pectin intake was associated with a shift in microbial taxa previously shown to be protective against CD onset in the GEM cohort; a reduction in Ruminococcus torques and an increase in NK4A214 group (phylum Firmicutes) presence (3.25×10−10&lt;q&lt;0.04).

Conclusion
This study demonstrates that dietary intake of fermentable fibre subtypes is associated with a reduced risk of CD development as well as with pleiotropic changes in CD risk biomarkers including microbiome, intestinal permeability, and subclinical inflammation. This suggests that the potential benefit of fermentable fibres on CD risk may involve changes in barrier function, and microbiome composition and function. Targeted interventions based on these findings could prove effective in preventing the onset of CD.