bluuebunny/crossref_metadata_2025_split huggingface dataset:
Hybrid light-matter chiral polariton state and the electromagnetic enantiomers
Authors: Kumar rahul , Trodden Ben , Klimash Anastasiia , Bousquet Manon , Fairbairn Nicola J. , Karimullah Affar , Gadegaard Nikolaj , Skabara Peter , Movsesyan Artur , Govorov Alexander , Hashiyada Shun , Hedley Gordon , kadodwala malcolm 
Published: June 2023

Abstract
Chiral electronic states of molecules, so-called enantiomers, have an intrinsic sense of handedness, or twist, and are the active component in energy efficient display technologies and in new photosynthetic routes to produce pharmaceuticals. Creating chiral states is achieved by manipulating the “twistiness” of the geometric molecular structure. This is a demanding problem in chemistry adding complexity due to the need to precisely control molecular geometry. Here we demonstrate a novel concept which decouples chirality from molecular geometry. Instead, it exploits the hybridisation of a non-chiral molecule with a chiral electromagnetic field, producing a hybrid light matter chiral polariton state. This is achieved by a symmetry-controlled strong chiral-light–matter interaction between an electromagnetic mode of a chiral nanocavity and an achiral molecule, a concept referred to as the electromagnetic-enantiomer. This electromagnetic mechanism simplifies the creation of chiral electronic states since it is far less demanding in terms of molecular design. We have illustrated the concept using an exemplar system relevant to display technology, producing efficient circularly polarised emission from a non-chiral emitter molecule.
Profiling antibody epitopes induced by mRNA-1273 vaccination and boosters
Authors: Girard Bethany , Baum-Jones Elisabeth , Best Rebecca , Campbell Thomas , Coupart Jack , Dangerfield Kyla , Dhal Abhilash , Jhatro Michael , Martinez Brian , Reifert Jack , Shon John , Zhang Minlu , Waitz Rebecca , Chalkias Spyros , Edwards Darin , Maglinao Maha , Paris Robert , Pajon Rolando 
Published: June 2023

Abstract
Characterizing the antibody epitope profiles of messenger RNA (mRNA)-based vaccines against SARS-CoV-2 can aid in elucidating the mechanisms underlying the antibody-mediated immune responses elicited by these vaccines. This study investigated the distinct antibody epitopes toward the SARS-CoV-2 spike (S) protein targeted after a 2-dose primary series of mRNA-1273 followed by a booster dose of mRNA-1273 or a variant-updated vaccine among serum samples from clinical trial adult participants. Multiple S-specific epitopes were targeted after primary vaccination; while signal decreased over time, a booster dose after >6 months largely revived waning antibody signals. Epitope identity also changed after booster vaccination in some subjects, with 4 new S-specific epitopes detected with stronger signals after boosting than with primary vaccination. Notably, the strength of antibody responses after booster vaccination differed by the exact vaccine formulation, with variant-updated mRNA-1273.211 and mRNA-1273.617.2 booster formulations inducing significantly stronger S-specific signals than a mRNA-1273 booster.
Movement Synchrony and Alliance in Problem-Focused and Solution-Focused Counseling
Authors: Hoffmann Christian , Gürtler Magdalene , Fendel Johannes , Lahmann Claas , Schmidt Stefan 
Published: June 2023

Abstract
The present study investigated the differences in movement synchrony and therapeutic alliance between solution-focused and problem-focused counseling. Thirty-four participants each attended two counseling sessions with different counselors, one with a solution-focus and one with a problem-focus, in randomized order. The sessions consisted of three consecutive parts: problem description, standardized intervention and free intervention. Movement synchrony, including leading and pacing synchrony, was measured using Motion Energy Analysis (MEA) and windowed cross-lagged correlation (WCLC) based on video recordings of the sessions. The Helping Alliance Questionnaire (HAQ) was used to assess therapeutic alliance. Results showed that movement synchrony was higher in solution-focused than in problem-focused counseling (p = .04), driven by differences in the problem description part. This difference may be attributed to allegiance, as we observed more leading synchrony during the problem description part in solution-focused sessions. There was no significant difference in therapeutic alliance between the two conditions. This study expands the understanding of counseling approaches in the field of behavioral synchrony and contributes valuable insights for practitioners and researchers alike.
Breeding corals for heat tolerance
Authors: Humanes Adriana , Lachs Liam , Beauchamp Elizabeth , Bukurou Leah , Buzzoni Daisy , Bythell John , Craggs Jamie , Torre Ruben de la , Edwards Alasdair , Golbuu Yimnang , Martinez Helios , Palmowski Pawel , Steeg Eveline van der , Sweet Michael , Ward Alex , Wilson Alastair , Guest James 
Published: June 2023

Abstract

Marine heatwaves are becoming more frequent, widespread and severe1, leading to mass coral bleaching and mortality. Yet it remains unknown whether natural coral adaptation can keep pace with climate warming2. As a result, selective breeding has been proposed to enhance coral heat tolerance3. The viability of this management solution hinges on the extent of heritability of coral heat tolerance. We show that selecting parent colonies for heat tolerance results in higher tolerance of adult offspring. This result held true for the response to both a 1-week and a 1-month simulated marine heatwave. In each case, narrow-sense heritability (h2) estimates are between 0.2 and 0.3, demonstrating a substantial genetic component to variation in heat tolerance. Given the variability in this population4, selective breeding could enhance heat tolerance by up to 1 °C-week within one generation. Contrary to expectations, we found no genetic correlation between short- and long-term heat stress tolerance. Selective breeding for short-stress tolerance did not enhance the ability of offspring to survive the long heat stress exposure, demonstrating that these traits are subject to independent genetic control. We demonstrate that selective breeding to enhance coral heat tolerance is feasible and a potential solution to help corals face ocean warming.
High prevalence group testing in epidemiology with geometrically inspired algorithms
Authors: Schenk Hannes , Caf Yasemin , Knabl Ludwig , Mayerhofer Christoph , Rauch Wolfgang 
Published: June 2023

Abstract
Demand for mass surveillance during peak times of the SARS‑CoV‑2 pandemic caused high workload for clinical laboratories. Efficient and cost conserving testing designs by means of group testing can substantially reduce resources during possible future emergency situations. The novel hypercube algorithm proposed by Mutesa et al. 2021 published in Nature provides methodological proof of concept and points out the applicability to epidemiological testing. In this work, the algorithm is explored and expanded for settings with high group prevalence. Numerical studies investigate the limits of the adapted hypercube methodology, allowing to optimize pooling designs for specific requirements (i.e. number of samples and group prevalence). Empirical validation was performed by elaborately pooling SARS‑CoV‑2 samples according to numerically optimized pooling designs. Laboratory experiments with SARS‑CoV‑2 sample groups, ranging from 50 to 200 items, characterized by group prevalence up to 10%, are successfully processed and analysed. Test‑reductions from 50% to 72.5% were achieved in the experimental setups when compared to individual testing. Higher theoretical test‑reduction is possible, depending on the number of samples and the group prevalence, indicated by simulation results.
Reef Structure of the Florida Reef Tract 2005-2020
Authors: Fisher William S. 
Published: June 2023

Abstract
Shallow-water coral reefs of the Florida Reef Tract comprise the third largest reef in the world, but during the last several decades scleractinian (stony) corals have suffered unprecedented declines from global and local stressors. A program to evaluate the effects of high-temperature bleaching events was initiated by The Nature Conservancy’s Florida Reef Resilience Program in 2005 and surveys have been completed across at least some portion of the entire region every year since. The program adopted a demographic (colony-based) assessment approach, which records colony species, size (height and maximum diameter) and estimated partial mortality (percent barren skeleton). Because reef structure is critical to ecosystem functioning and services, data from 2005–2020 were analyzed to describe the abundance, size and morphological complexity of stony coral colonies forming the biogenic reef. Colony height, footprint, surface area and volume summed for each of 6,016 transects were used to describe reef structure and averages were used to characterize the components that contributed to the structure. Nearly 150,000 colonies representing 49 species were reported during this period and results demonstrated both spatial and temporal changes for the region and for geographic subregions. Some subregions showed increasing colony density, especially for three small, hemispheric species, and declining average colony size. Colony dimensions allowed quantitative estimation of some ecosystem goods and services, such as fish habitat and carbon storage.
Development and Validation of the ADHD Symptom and Side Effect Tracking - Baseline Scale (ASSET-BS): A Novel Short Screening Measure for ADHD in Clinical Populations
Authors: Young Joel L. , Powell Richard N. , Zabel Celeste , Saal Jaime , Welling Lisa L. M. , Fortain Jillian , Ceresnie Ashley 
Published: June 2023

Abstract
Objective The aim was to develop and test a novel screen of adult ADHD, with a focus on clinical use. We designed a series of three studies to accomplish this aim.
Method Study One (n = 155) and Study Two (n = 591) collected data via surveys to conduct exploratory and confirmatory factor analyses respectively. Study Three analyzed the scale's psychometrics in a clinical sample (n = 151).
Results Study One and Study Two identified a 10-item scale with a two-factor structure. Study Three found good discriminant validity, sensitivity = 80.0%, specificity = 80.2%, and convergent validity with the Brown Executive Function/Attention Scales, r (131) = .76, p < .001, and the Conner’s Adult ADHD Rating Scales r (131) = .71, p < .001. Conclusion The scale demonstrated effectiveness in screening for ADHD in a psychiatric outpatient population. Its results may be used to identify patients that may benefit from thorough ADHD diagnostic procedures. Keywords: adult ADHD, assessment, decision making
Extraction of a metallic susceptor after accidental ingestion of the heated tobacco product TEREA™: a case report
Authors: Higashi Koki , Koike Yuhki , Sato Yuki , Yamashita Shinji , Nagano Yuka , Shimura Tadanobu , Kitajima Takihito , Matsushita Kohei , Yokota Kazuki , Amano Keishiro , Okugawa Yoshinaga , Toiyama Yuji 
Published: June 2023

Abstract
Background: Tobacco ingestion is widely known to cause nicotine toxicity, which may result in severe symptoms. Two heated tobacco products, called TEREA™ and SENTIA™, were launched in 2021 by Philip Morris International (New York, NY, USA), and their ingestion is associated with a risk of bowel injury because they contain a partially pointed metallic susceptor. However, this risk is not well known to the general public or healthcare providers. To increase awareness of this risk, we herein report a case involving extraction of a metallic susceptor after ingestion of the heated tobacco product TEREA™.
Case presentation: A 7-month-old girl presented to the emergency department of a nearby hospital because she was suspected to have accidentally swallowed heated tobacco. Although she presented with no symptoms related to nicotine poisoning, abdominal X-ray examination revealed a metal object in her stomach. According to a statement released by the Japan Poison Information Center, the TEREA™ heated tobacco product contains a metallic susceptor with a rectangular shape and sharp corners. The patient was transferred to our department because of the risk of bowel injury, and upper gastrointestinal endoscopy was performed. No cigarettes were found by endoscopic observation; however, a metallic susceptor was located in the second part of the duodenum. We grasped it with biopsy forceps and carefully removed it using an endoscope with a cap attached to the tip. The postoperative course was uneventful.
Conclusions: Some patients who ingest heated tobacco might be exposed not only to the effects of nicotine but also to physical damage caused by a metallic susceptor. Clinicians should pay attention to this risk in the clinical setting.
Sorcin regulate pyroptosis by interacting with NLRP3 inflammasomes to facilitate the progression of hepatocellular carcinoma
Authors: li zhenfen , yang Ziyue , Zhu Yuanyuan , Fu Chunmeng , Li Ning , peng Fang 
Published: June 2023

Abstract
A high recurrence rate and easy metastasis are two prominent clinical features of hepatocellular carcinoma (HCC), which is also the most common cause of cancer-related death. However, the molecular pathogenesis of HCC remains unclear. Soluble resistance-related calcium-binding protein (Sorcin) is highly expressed in a variety of tumor cell lines and multidrug-resistant cell lines and participates in the malignant progression of tumors by regulating apoptosis. Pyroptosis is also a form of programmed cell death that plays a crucial role in exerting tumor suppression function and evoking anti-tumor immune responses. However, there is no consensus that Sorcin promotes HCC progression by regulating pyroptosis. Our study manifested that Sorcin was considerably upregulated, whereas pyroptosis-associated proteins were significantly decreased in HCC tissues and cells. Sorcin silencing attenuated the proliferation, migration, and invasion of HCC cells. Knockdown of Sorcin activates pyroptosis, and overexpression of Sorcin inhibits pyroptosis, yet has no significant effect on apoptosis, ferroptosis, and autophagy in HCC cells. Furthermore, coimmunoprecipitation and immunofluorescence assays revealed that Sorcin interacted with NLRP3 inflammasomes to regulate pyroptosis in HCC cells. Then, the NLRP3 inhibitor MCC950 inhibited the activation of Sorcin knockdown-induced pyroptosis and reversed the effect of Sorcin silencing-induced weakening of malignant biological behavior in HCC. Similarly, suppression of Caspase-1 reversed the inhibitory effect of Sorcin knockdown on the malignant progression of HCC via knockdown of Caspase-1 or the inhibitor VX765. Consistent with the in vitro results, the nude mouse experiment showed that Sorcin knockdown inhibited the growth of HCC by activating pyroptosis, while Caspase-1 knockdown partially restored the growth inhibition caused by Sorcin-knockdown. Collectively, high Sorcin expression in HCC negatively regulates pyroptosis by interacting with the NLRP3 inflammasome to promote HCC proliferation, migration, and invasion. The results of this study provide a scientific basis for Sorcin as a new biomarker and potential therapeutic target for HCC.
Cryptic diversity in sympatric Migonemyia migonei (Diptera: Psychodidae: Phlebotominae), eventual meaning for leishmaniasis transmission
Authors: Moya Sofía Lorián , Pech-May Angélica , Quintana María Gabriela , Salomón Oscar Daniel 
Published: June 2023

Abstract
Migonemyia migonei (Franҫa 1920) is a Phlebotominae of epidemiological importance due to its role as a vector in leishmaniasis transmission cycles and its broad geographic distribution in South America. A few morphometric and genetic studies have demonstrated the existence of variability between geographically distant populations in Brazil. The aim of the study was the estimation of the genetic distance in the morphospecies Mg. migonei through the analysis of Cytochrome C oxidase subunit I (COI) sequences of specimens captured in Argentina and those available in online databases. COI sequences from specimens collected in different localities of Argentina and sequences available in online databases were used. Genetic distances were analyzed and a median-joining haplotype network constructed. Finally, a phylogenetic reconstruction was done according to Bayesian inference. The analyses allowed the identification of at least two haplogroups: haplogroup I with sequences of specimens from Colombia, Brazil and Argentina and haplogroup II with sequences of specimens from Argentina. Interestingly, specimens from Argentina whose haplotypes corresponded to both haplogroups were collected in sympatry. The results suggest that Mg. migonei could be a species complex with at least two distinct members. This hypothesis would explain the known characteristics of adaptability and vector permissiveness of the species, as the putative cryptic species of the complex could differ in traits of epidemiological importance.
CNTN4/APP axis of cancer cells and T-cells
Authors: Jeon Bu-Nam , Kim Sujeong , Kim Yunjae , Yu Hyunkyung , Kim Hyunuk , Ha Youngeun , Kim Yun Yeon , Park Changho , Kim Gihyeon , Cha Miyoung , Palucka Karolina , Lee Charles , Park Hansoo 
Published: June 2023

Abstract
Immune checkpoint inhibitors have significantly advanced tumor treatment, but their limited benefits and strong responses in only a subset of patients persist as challenges. CNTN4, a neuronal membrane protein involved in cell adhesion and synapse signaling, has unclear immunomodulatory functions. In this study, we reveal the immune checkpoint role of CNTN4 in T-cell proliferation and activation both in vitro and in vivo. We found that CNTN4, highly expressed in numerous tumor tissues, impedes T-cell proliferation, cytotoxicity, and the secretion of cytotoxic cytokines in vitro. On T cells, CNTN4 binds to two APP isoforms, APP770 and APP751, which results in attenuated TCR signaling and diminished cell adhesion capacity. To target this interaction, we developed GENA-104A16 against CNTN4 and an anti-APP antibody (5A7) that blocks the binding between CNTN4 and APP. Administering these two antibodies demonstrated anti-tumor effects in a syngeneic tumor mouse model and increased tumor-infiltrating lymphocytes within tumor tissues in vivo. Furthermore, elevated CNTN4 levels are associated with poor prognosis and negatively correlated with various cytotoxic immune-related markers. In conclusion, CNTN4 serves as a bona fide immune checkpoint protein and represents a promising therapeutic target for developing immunotherapeutic drugs.
Combined with dynamic serum proteomics and clinical follow-up to screen the serum proteins to promote the healing of diabetic foot ulcer
Authors: Zhao Fengying , Xie Liangxiao , Weng Zhiyan , Huang Yihong , Zheng Lifeng , Yan Sunjie , Shen Ximei 
Published: June 2023

Abstract

ObjectiveNon-healing diabetic foot ulcers are a leading cause of disability and death in diabetic patients, which often results in lower limb amputation. This study aimed to investigate the impact of biomarkers on the healing of diabetic foot ulcers by utilizing dynamic serum proteomics and skin proteomic analysis, combined with clinical case follow-up studies.MethodsTo analyze dynamic serum proteomic changes in four groups, age-matched normal subjects, diabetic patients, pre-treatment diabetic foot ulcer patients, and healed diabetic foot ulcer patients were selected. The differential proteins were screened in conjunction with normal and diabetic foot ulcer skin proteomics. In this study, a total of 80 patients with diabetic foot ulcers were enrolled and monitored for 3–6 months during treatment. To verify the significance of the differential proteins, age-matched diabetic patients (240 patients) and healthy controls (160 patients) were included as controls.ResultsDynamic serum proteomics trend showed that the level of negative regulatory proteins related to endothelial cell migration, angiogenesis, and vascular development was significantly decreased after treatment of diabetic foot ulcer. This study also found that there is an opposite trend of differential protein biological processes, which is mainly enriched in protein activation cascade, immunoglobulin production, and complement activation. The researchers identified the core proteins APOA1, LPA, and APOA2 through a convergence of serum and skin proteomics screening. Clinical cases further validated that APOA1 levels are decreased in diabetic foot ulcer patients and are correlated with disease severity.ConclusionsBased on our dynamic proteomics and clinical case studies, our bioinformatic analysis suggests that APOA1 plays a critical role in linking coagulation, inflammation, angiogenesis, and wound repair, making it a key protein that promotes the healing of diabetic foot ulcers.
Palaeoatmosphere facilitates a gliding transition to powered flight in Eocene bats
Authors: Cannell Alan , Amador Lucila , Simmons Nancy , GIANNINI NORBERTO 
Published: June 2023

Abstract
Bats are the only flying mammals but their transition to flight remains poorly understood and controversial. We applied aerodynamic modeling to reconstruct flight in the oldest complete fossil bat, the archaic Onychonycteris finneyi from the early Eocene of North America, under paleo-atmospheric conditions. Results indicate that Onychonycteris was capable of both gliding and powered flight either in a standard normodense aerial medium or in a hyperdense atmosphere estimated for the Eocene from two independent paleogeochemical proxies. Aerodynamic continuity across a morphological gradient is demonstrated by modeled intermediate forms with increasing aspect ratio (AR) produced by gradual digital elongation, such as indicated by chiropteran developmental data. Here a performance gradient emerged of decreasing sink rate with increasing AR that eventually allowed available muscle power to achieve level flight using flapping, a process greatly facilitated under elevated atmospheric densities. This gradient strongly supports a gliding (trees-down) transition to powered flight in bats.
Regulation of Med1 protein by overexpression of BAP1 in breast cancer cells
Authors: Kim Hyunju 
Published: June 2023

Abstract

Med1, a subunit of the TRAP/mediator complex acts as a transcriptional regulatory mediator, binds to a nuclear receptor and regulated transcription. It is known that elevated Med1 protein expression promotes cancer growth in hormone-dependent breast and prostate cancer, whereas low expression of Med1 protein in melanoma and lung cancer increases cancer metastasis. In this study, Med1 protein expression by deubiquitinating enzymes (DUBs) overexpression was investigated in breast and lung cancer cell lines. Overexpression of BAP1 protein did not affect Med1 mRNA expression in breast cancer and lung cancer cell. However, Med1 protein expression decreased in breast cancer cells while increased in lung cancer cell lines. In addition, Med1 protein expression by overexpression of BAP1 mutant (C91A) in breast and lung cancer cells was not affected. Increased BAP1 expression increased cell growth and metastatic capacity in lung cancer cells. And overexpression of BAP1 in breast cancer cell lines increased the transcriptional regulation of estrogen receptor (ER). In addition, the binding between the Med1 and the BAP1 protein was observed. Therefore, these data suggested that BAP1 was involved in cancer cell growth and metastasis by binding to Med1 protein and regulating Med1 protein expression depending on the cancer cell types.