bluuebunny/crossref_metadata_2025_split huggingface dataset:
Expression of FBXW11 in normal and disease‐associated osteogenic cells
Authors: Dalle Carbonare Luca , Gomez Lira Macarena , Minoia Arianna , Bertacco Jessica , Orsi Silvia , Lauriola Angela , Li Vigni Veronica , Gandini Alberto , Antoniazzi Franco , Zipeto Donato , Mottes Monica , Bhandary Lekhana , Guardavaccaro Daniele , Valenti Maria Teresa 
Published: May 2023

AbstractThe ubiquitin‐proteasome system (UPS) plays an important role in maintaining cellular homeostasis by degrading a multitude of key regulatory proteins. FBXW11, also known as b‐TrCP2, belongs to the F‐box family, which targets the proteins to be degraded by UPS. Transcription factors or proteins associated with cell cycle can be modulated by FBXW11, which may stimulate or inhibit cellular proliferation. Although FBXW11 has been investigated in embryogenesis and cancer, its expression has not been evaluated in osteogenic cells. With the aim to explore FBXW11gene expression modulation in the osteogenic lineage we performed molecular investigations in mesenchymal stem cells (MSCs) and osteogenic cells in normal and pathological conditions. In vitro experiments as well as ex vivo investigations have been performed. In particular, we explored the FBXW11 expression in normal osteogenic cells as well as in cells of cleidocranial dysplasia (CCD) patients or osteosarcoma cells. Our data showed that FBXW11 expression is modulated during osteogenesis and overexpressed in circulating MSCs and in osteogenically stimulated cells of CCD patients. In addition, FBXW11 is post‐transcriptionally regulated in osteosarcoma cells leading to increased levels of beta‐catenin. In conclusion, our findings show the modulation of FBXW11 in osteogenic lineage and its dysregulation in impaired osteogenic cells.
Challenges and opportunities for bioimage analysis core‐facilities
Authors: Soltwedel Johannes Richard , Haase Robert 
Published: May 2023

AbstractRecent advances in microscopy imaging and image analysis motivate more and more institutes worldwide to establish dedicated core‐facilities for bioimage analysis. To maximise the benefits research groups at these institutes gain from their core‐facilities, they should be established to fit well into their respective environment. In this article, we introduce common collaborator requests and corresponding potential services core‐facilities can offer. We also discuss potential competing interests between the targeted missions and implementations of services to guide decision makers and core‐facility founders to circumvent common pitfalls.
Forecasting seed production in perennial plants: identifying challenges and charting a path forward
Authors: Journé Valentin , Hacket‐Pain Andrew , Oberklammer Iris , Pesendorfer Mario B. , Bogdziewicz Michał 
Published: May 2023

SummaryInterannual variability of seed production, known as masting, has far‐reaching ecological impacts including effects on forest regeneration and the population dynamics of seed consumers. Because the relative timing of management and conservation efforts in ecosystems dominated by masting species often determines their success, there is a need to study masting mechanisms and develop forecasting tools for seed production. Here, we aim to establish seed production forecasting as a new branch of the discipline. We evaluate the predictive capabilities of three models – foreMast, ΔT, and a sequential model – designed to predict seed production in trees using a pan‐European dataset of Fagus sylvatica seed production. The models are moderately successful in recreating seed production dynamics. The availability of high‐quality data on prior seed production improved the sequential model's predictive power, suggesting that effective seed production monitoring methods are crucial for creating forecasting tools. In terms of extreme events, the models are better at predicting crop failures than bumper crops, likely because the factors preventing seed production are better understood than the processes leading to large reproductive events. We summarize the current challenges and provide a roadmap to help advance the discipline and encourage the further development of mast forecasting.
Safety and Efficacy of Cortical Bone Trajectory Screw Fixation Combined with Facet Fusion for the Treatment of Lumbar Degenerative Disease
Authors: Kong Xiaoqian , Li Bohan , Xu Leixin , Li Jianmin , Gao Chunzheng , Wu Dongjin , Ma Shengzhong , Song Yang , Zhao Jie , Wang Xiaoyun 
Published: May 2023

ObjectiveThe mainstream lumbar fusion surgeries have various shortcomings, such as complex operation, much invasion, and loss of lumbar function. How to minimize the surgical injury and to achieve better therapeutic effects has become the goal pursued by spine surgeons. This study introduces a cortical bone trajectory (CBT) screw fixation combined with facet fusion (FF), evaluates its safety and efficacy, and explores its advantages, in order to provide a reference for treatment of patients with single‐level lumbar stenosis or grade I degenerative spondylolisthesis.MethodsWe retrospectively analyzed the clinical, radiological, and operative data of 167 patients with single‐level lumbar stenosis or grade I degenerative spondylolisthesis who underwent FF or transforaminal lumbar interbody fusion (TLIF) from January 2013 to September 2019 in the spine surgery department of the Second Hospital of Shandong University. Patients were divided into four groups according to surgical method: group CBT‐FF, CBT screw combined with FF; group PS‐FF, pedicle screw (PS) combined with FF; group CBT‐TLIF, CBT screw combined with TLIF; and group PS‐TLIF, PS combined with TLIF. The operation time, estimated intraoperative blood loss, complications after surgery, visual analog scale (VAS), and Oswestry disability index (ODI) of the four groups were compared. The fusion was evaluated by anteroposterior and lateral X‐ray, CT scan, and three‐dimensional reconstruction.ResultsTwelve months after surgery, the fusion rate of four groups had no significantly statistical differences (p = 0.914). VAS and ODI scores were lower after surgery than before. Low back pain VAS scores 1 week after surgery in group CBT‐FF and group CBT‐TLIF were significantly lower than those in group PS‐FF and group PS‐TLIF (pCF/PF = 0.001, pCF/PT = 0.000, pPF/CT = 0.049, pCT/PT = 0.000). Low back pain VAS score 3 months after surgery was significantly lower in group CBT‐FF than group PS‐FF and group PS‐TLIF (pCF/PF = 0.045, pCF/PT = 0.008). ODI score 1 week after surgery was significantly lower in group CBT‐FF than group PS‐FF, group CBT‐TLIF, and group PS‐TLIF (pCF/PF = 0.000, pCF/CT = 0.005, pCF/PT = 0.000, pCT/PT = 0.015). ODI score 3 months after surgery was significantly lower in group CBT‐FF than group PS‐FF, group CBT‐TLIF, and group PS‐TLIF (pCF/PF = 0.001, pCF/CT = 0.002, pCF/PT = 0.000). Incidence of complications did not significantly differ among the groups.ConclusionCBT screw fixation combined with FF is a safe and efficacious procedure for patients with single‐level lumbar stenosis or grade I degenerative spondylolisthesis. This minimally invasive approach of lumbar fusion can be simply and easily performed. Patients who undergo CBT screw fixation combined with FF recovered faster than TLIF.
Natural born violence? Understanding street‐level bureaucrats' use of violence: Police officers and protests
Authors: Alcadipani Rafael , Lotta Gabriela , Cohen Nissim 
Published: May 2023

AbstractWhat factors influence street‐level bureaucrats' (SLBs) use of violence? To answer this question, we focus on police officers, a typical example of SLBs, who can lawfully use violence whenever they deem it necessary. Based on ethnographic work among Brazilian police officers dealing with protests, we analyze how violence erupts in the interaction between police officers and protesters. We contribute to the literature by suggesting that no single factor alone can explain the actions of SLBs regarding the use of violence, as previous research has posited. We demonstrate how the interplay between four factors explains the use of violence by SLBs: (1) institutional logic, (2) type of situation, (3) desire for revenge and having fun, and (4) lack of accountability. As such, we propose a more complex account of SLB's use of violence.
Disasters and social vulnerability determinants of federal subsidiarity assistance
Authors: Ahmadu Aisha S. , Nukpezah Julius A. , French P. Edward , Menifield Charles E. 
Published: May 2023

AbstractSubsidiarity assistance creates opportunities for the federal government to intervene in subnational affairs, supplement emergency response, and reduce jurisdictional vulnerabilities. Recognizing the differential effects that disaster events and revenue sharing could have on states, the research investigates the determinants of federal subsidiarity assistance grants to states in the context of American federalism. This study draws from disaster impact scholarship and social vulnerability theory for theoretical context to understand the determinants of short‐term and long‐term public assistance grants and intergovernmental transfers, which constitute federal subsidiarity assistance to subnational governments. Using panel data on 50 U.S. states over a 17‐year time period, findings show that disasters trigger federal subsidiarity assistance for disaster‐induced short‐term and long‐term public assistance grants; however, social vulnerability more likely explains intergovernmental transfers to states.
Are special districts strategic complements or strategic substitutes?
Authors: Goodman Christopher B. , Carroll Deborah A. 
Published: May 2023

AbstractWhat happens to general‐purpose local government spending for service provision when a special district enters the public service market? Theoretically, special districts can act either as complements by supplementing existing service provision spending or as substitutes by supplanting current service provision spending. We find a substitution effect using fixed effects regression on urban counties in the United States from 1972 to 2017. Special districts replace spending for public service provision by county governments; however, we find no similar result for municipal governments. But the results are nuanced—findings are confined mainly to public services that tend to cover large land areas, like fire protection, sewerage, and solid waste management. Furthermore, we find evidence that day‐to‐day operations drive observed substitution, and that county size is an important factor depending on the functional service area.
Parent training for 842 families experiencing disruptive child behavior across 19 Danish community sites: A benchmark effectiveness study
Authors: Greve Lea T. , Fentz Hanne N. , Trillingsgaard Tea 
Published: May 2023

Interactional processes between child and parents, in particular harsh and coercive parenting practices, have been established as important factors maintaining and shaping the developmental trajectory of disruptive child behavior. The Incredible Years Parent Training (IYPT) is a well‐established evidence‐based program targeting negative parent‐child interactions in families with children exhibiting high levels of disruptive behaviors. There are, however, few studies investigating the effectiveness of the IYPT when implemented in established practice settings independently from research environments. Also, very little evidence exists for the program's effectiveness for school‐aged children. The IYPT was administered to consecutive groups of parents (N = 842) across 19 Danish community sites during the period from 2012 to 2019. Pre and post data were collected using the Eyberg Child Behavior Inventory (ECBI). By means of a benchmark approach, the effectiveness of the intervention was compared with that of two European effectiveness randomized controlled trials. Large effect sizes were seen from pre to post for both parent‐reported number of problematic disruptive child behaviors (ECBI Problem subscale; d = 1.51, p = 0.000, 95% CI [9.06–10.01]) and frequency of these behaviors (ECBI Intensity subscale; d = 1.15, p = 0.000, 95% CI [29.33–32.73]). Treatment effects found in this study are comparable to or larger than those found in previous effectiveness studies and show the IYPT to be an effective intervention when implemented across a range of different community settings in a large community sample of children across an age span of 2 to 12 years.
Pseudophosphatase STYXL1 depletion enhances glucocerebrosidase trafficking to lysosomes via ER stress
Authors: Patel Saloni , Bhatt Anshul Milap , Bhansali Priyanka , Setty Subba Rao Gangi 
Published: May 2023

AbstractPseudophosphatases are catalytically inactive but share sequence and structural similarities with classical phosphatases. STYXL1 is a pseudophosphatase that belongs to the family of dual‐specificity phosphatases and is known to regulate stress granule formation, neurite formation and apoptosis in different cell types. However, the role of STYXL1 in regulating cellular trafficking or the lysosome function has not been elucidated. Here, we show that the knockdown of STYXL1 enhances the trafficking of β‐glucocerebrosidase (β‐GC) and its lysosomal activity in HeLa cells. Importantly, the STYXL1‐depleted cells display enhanced distribution of endoplasmic reticulum (ER), late endosome and lysosome compartments. Further, knockdown of STYXL1 causes the nuclear translocation of unfolded protein response (UPR) and lysosomal biogenesis transcription factors. However, the upregulated β‐GC activity in the lysosomes is independent of TFEB/TFE3 nuclear localization in STYXL1 knockdown cells. The treatment of STYXL1 knockdown cells with 4‐PBA (ER stress attenuator) significantly reduces the β‐GC activity equivalent to control cells but not additive with thapsigargin, an ER stress activator. Additionally, STYXL1‐depleted cells show the enhanced contact of lysosomes with ER, possibly via increased UPR. The depletion of STYXL1 in human primary fibroblasts derived from Gaucher patients showed moderately enhanced lysosomal enzyme activity. Overall, these studies illustrated the unique role of pseudophosphatase STYXL1 in modulating the lysosome function both in normal and lysosome‐storage disorder cell types. Thus, designing small molecules against STYXL1 possibly can restore the lysosome activity by enhancing ER stress in Gaucher disease.
Benchmarking of protein interaction databases for integration with manually reconstructed signalling network models
Authors: Van de Graaf Matthew W. , Eggertsen Taylor G. , Zeigler Angela C. , Tan Philip M. , Saucerman Jeffrey J. 
Published: May 2023

AbstractProtein interaction databases are critical resources for network bioinformatics and integrating molecular experimental data. Interaction databases may also enable construction of predictive computational models of biological networks, although their fidelity for this purpose is not clear. Here, we benchmark protein interaction databases X2K, Reactome, Pathway Commons, Omnipath and Signor for their ability to recover manually curated edges from three logic‐based network models of cardiac hypertrophy, mechano‐signalling and fibrosis. Pathway Commons performed best at recovering interactions from manually reconstructed hypertrophy (137 of 193 interactions, 71%), mechano‐signalling (85 of 125 interactions, 68%) and fibroblast networks (98 of 142 interactions, 69%). While protein interaction databases successfully recovered central, well‐conserved pathways, they performed worse at recovering tissue‐specific and transcriptional regulation. This highlights a knowledge gap where manual curation is critical. Finally, we tested the ability of Signor and Pathway Commons to identify new edges that improve model predictions, revealing important roles of protein kinase C autophosphorylation and Ca2+/calmodulin‐dependent protein kinase II phosphorylation of CREB in cardiomyocyte hypertrophy. This study provides a platform for benchmarking protein interaction databases for their utility in network model construction, as well as providing new insights into cardiac hypertrophy signalling.
imageKey points
Protein interaction databases are used to recover signalling interactions from previously developed network models.
The five protein interaction databases benchmarked recovered well‐conserved pathways, but did poorly at recovering tissue‐specific pathways and transcriptional regulation, indicating the importance of manual curation.
We identify new signalling interactions not previously used in the network models, including a role for Ca2+/calmodulin‐dependent protein kinase II phosphorylation of CREB in cardiomyocyte hypertrophy.
Comparison of Operation and Maintenance of Floating 14 MW Turbines and Twin 10 MW Turbines
Authors: Avanessova Nadezda , Land James , Lee Alistair , Lazakis Iraklis , Thomson Camilla 
Published: May 2023

Abstract
Turbine ratings in the past decade have grown unexpectedly fast. In 2021, Siemens Gamesa and GE revealed their new 14 MW turbine models, and it is predicted that this is not yet the rating limit that turbines can reach. Increased turbine ratings can also be achieved by putting two turbines on a single foundation. This study analyzes how operation and maintenance (O&M) would differ if a floating wind farm had twin 10 MW turbines installed on each substructure, instead of a single 14 MW turbine. This study demonstrates how the strategic O&M simulation tool compass can be used to perform this comparison. Assumptions regarding the O&M of twin turbines were estimated with the major floating twin turbine developer Hexicon AB. This study analyzed four cases—a case with 35 twin 10 MW turbines, and three cases with 50 single 14 MW turbines—to understand the potential effect of increased consumable costs, spare part lead times, and maintenance durations. All cases had the same wind farm capacity of 700 MW. The results show that O&M for cases with single turbines is at least 4.5% more expensive than the case with twin turbines. The case with twin turbines also resulted in a higher availability than any other case. Additionally, results showed that operational expenditure (OPEX) for the cases with single turbines is at least 6.0% higher in scenarios with single turbines than in the twin turbine scenario. The biggest cost contributors to the difference between scenarios were craft costs, particularly cable laying vessels and tugs. Due to the higher number of cables required for the scenario with single turbines, there is more frequent mobilization of cable vessels for cable repairs.
Experimental Analysis of the Influential Factors on Mixed Convection Flow in Horizontal Pipes
Authors: Sharma Amrita , Sontakke Smita , Kothadia Hardik B. , Singh Shobhana , Mondal Bobin 
Published: May 2023

Abstract
It has been speculated that a forced pipe flow is always assisted by free convection owing to the dependency of fluid properties on its temperature. The purpose of the current study is to experimentally examine the effect of different-sized smooth horizontal pipes on mixed convection of water in internal flows under uniform heat flux (UHF) wall conditions. Infrared thermal imaging is used to measure outer surface temperature in axial and circumferential directions. Reynolds number range is taken between 1000 and 18,000 on three test sections of the diameter of 8 mm, 13.8 mm, and 17.8 mm. The outcome of varying tube diameter, mass flux, and heat flux on mixed flow characteristics is studied. The strength of free convection is illustrated by the ratio of top to bottom local heat transfer coefficient. It is found to be maximum at the tube outlet by 50% and 80% for 8 mm and 13.8 mm tube diameter than the inlet. This enhanced the laminar Nusselt number by 3 to 6 times the analytical value of Nu = 4.36 under UHF condition. The Nusselt number increases with the increase in the tube diameter. The Nusselt number increased by 36% when the surface area increased from a tube diameter of 8 mm to 17.8 mm. Also, the temperature distribution in the turbulent regime remains constant from the highest point to the bottom point. However, it significantly differs in laminar flow. A suitable correlation is suggested for the variation of the Nusselt number under the laminar regime showing the emphasis of free convection on forced convection.
Effect of Rib Blockage Ratio and Arrangements on Impingement Heat Transfer in Double-Wall Cooling
Authors: Kong Dehai , Ma Zhenyuan , Li Wei , Guo Tao , Liu Cunliang 
Published: May 2023

Abstract
A numerical study was conducted on the multiple jet impingement heat transfer of the double-wall cooling with high blockage ratio ribs of various configurations. Three different blockage ratios (BR = 0.2, 0.3, and 0.5) and two rib arrangements relative to the effusion holes (l/Sx = 0.25 and 0.75) were thoroughly examined using the RANS method with the SST–KIC turbulence model, considering the Kato–Launder modification (K), intermittency (I), and crossflow (C) transition effects. The ratio of jet-to-target plate spacing to jet diameter (H/d) was fixed to be 1, and Reynolds numbers varied in the range of 4000–16,000. Furthermore, the computed data on the rib roughed wall were also compared with those on a flat plate for the double-wall cooling. The results demonstrate that the installation of the high blockage ribs significantly decreases the detrimental crossflow effect due to the blockage effect of the ribs and intensively disturbs the jet flow in flow passing over the ribs, thereby enhancing the heat transfer performance. For the impingement/effusion cooling, the arrangement of the rib downstream of the effusion holes (l/Sx = 0.25) shows more advantages, and the heat transfer level rises quickly as BR increases. The best thermal-hydraulic performance and heat transfer uniformity on the rib roughed target plate is both obtained at BR = 0.3, which can be increased by up to 10% and 8%, respectively, compared to those on the flat plate.