bluuebunny/crossref_metadata_2025_split huggingface dataset:
Evaluating extremophilic microorganisms in industrial regions
Authors: Asyakina Lyudmila , Vorob'eva Ekaterina , Proskuryakova Larisa , Zharko Maria 
Published: February 2023

Abiotic and biotic stresses have a major impact on crop growth. Stress affects the root system and decreases the amount of nutrients in fruits. Modern agricultural technologies help replace mineral fertilizers with new generation biopreparation. Unlike chemical fertilizers, biofertilizers reduce the risk of adverse environmental impacts. Of special interest are extremophilic microorganisms able to survive in extreme conditions. We aimed to study the phytostimulating ability of extremophilic bacteria isolated from disturbed lands in the coal-mining region.
We isolated microorganisms from disturbed lands and studied their cultural, morphological, and biochemical properties. Then, we determined their ability to synthesize indole-3-acetic acids. The extremophilic bacteria were identified and subjected to biocompatibility testing by co-cultivation. Next, we created consortia of pure cultures and analyzed biomass growth. Finally, the biopreparation was experimentally tested on Trifolium prantense L. seeds.
We isolated 10 strains of microorganisms that synthesized 4.39 to 16.32 mg/mL of indole-3-acetic acid. The largest amounts of the acid were produced by Pantoea spp., Enterococcus faecium, Leclercia spp., Rothia endophytica, and Klebsiella oxytoca. A consortium of Pantoea spp., E. faecium, and R. endophytica at a ratio of 1:1:1 produced the largest amount of indole-3-acetic acid (15.59 mg/mL) and accumulated maximum biomass. The addition of 0.2% L-tryptophan to the nutrient medium increased the amount of indole-3-acetic acid to 18.45 mg/mL. When the T. prantense L. seeds were soaked in the biopreparation (consortium’s culture fluid) at a concentration of 2.5, the sprouts were 1.4 times longer on the 10th day of growth, compared to the control.
The consortium of Pantoea spp., E. faecium, and R. endophytica (1:1:1) stimulated the growth of T. prantense L. seeds. Our findings can be further used to develop biofertilizers for agriculture.
Cognitive Benefits of Sodium-Glucose Co-Transporters-2 Inhibitors
in the Diabetic Milieu
Authors: Yaribeygi Habib , Maleki Mina , Sathyapalan Thozhukat , Rizzo Manfredi , Sahebkar Amirhossein 
Published: February 2023

Abstract:
Patients with diabetes are at higher risk of cognitive impairment and memory
loss than the normal population. Thus, using hypoglycemic agents to improve brain function
is important for diabetic patients. Sodium-glucose cotransporters-2 inhibitors (SGLT2i)
are a class of therapeutic agents used in the management of diabetes that has
some pharmacologic effects enabling them to fight against the onset and progress of memory
deficits. Although the exact mediating pathways are not well understood, emerging
evidence suggests that SGLT2 inhibition is associated with improved brain function.
This study reviewed the possible mechanisms and provided evidence suggesting SGLT2
inhibitors could ameliorate cognitive deficits.
Biological Activities of Morita-Baylis-Hillman Adducts (MBHA)
Authors: Paiva Ferreira Larissa Adilis Maria , de Lima Louise Mangueira , Paiva Ferreira Laercia Karla Diega , Bernardo Larissa Rodrigues , Castro Aleff , Lima Junior Claudio Gabriel , de Almeida Vasconcellos Mário Luiz Araújo , Piuvezam Marcia Regina 
Published: February 2023

Background:
The Morita-Baylis-Hillman reaction (MBHR) is considered one of the most
powerful and versatile methodologies used for carbon-carbon bond formation. The reaction is defined
as the condensation between an electrophilic carbon sp² and the α position of an olefin, carrying an
electron-withdrawing group, in the presence of a catalyst. The advantages of the reaction are the high
atom economy and mild reaction conditions. Under ideal conditions, this reaction leads to the formation
of multifunctional products, called Morita-Baylis-Hillman adducts (MBHA), a class of relevant
molecules that exhibit a variety of biological activities.

Objective:
Considering the importance of these compounds, this review brought together several studies
regarding the biological activities of MBHA, to point out the use of these molecules as future therapeutic
agents.

Methods:
We searched for scientific articles available in the main databases, published between 1999
and 2022, using the descriptors: Morita-Baylis-Hillman adducts, Morita-Baylis-Hillman reaction, biological
activity, and biological potentiality.

Results:
Thirty-five articles showed the variety of biological activities of MBHA, including molluscicidal,
antitumor, herbicidal, and fungicidal, antileishmanial, antioxidant, antimalarial, anti-tumor inflammatory,
vasorelaxant, antichagasic, antimicrobial, and anti-inflammatory activities.

Conclusion:
Therefore, these compounds are promising candidates to become drugs for the treatment
of a variety of diseases, following further studies to understand the effective mechanisms of action of
MBHA.
APOE4: A Culprit for the Vulnerability of COVID-19 in Alzheimer's
Patients
Authors: Agrawal Neetu , Goyal Ahsas , Kushwah Prashant Singh , Pathak Shilpi 
Published: February 2023

Abstract:
Apolipoprotein E4 (APOE4) is one of the primary genetic risk factors for late-onset of
Alzheimer's disease (AD). While its primary function is to transport cholesterol, it also regulates
metabolism, aggregation, and deposition of amyloid-β (Aβ) in the brain. The disruption in the generation
and removal of Aβ in the brain is the primary cause of memory and cognitive loss and thus
plays a significant role in the development of AD. In several prior genetic investigations, the APOE4
allele has been linked to higher susceptibility to severe acute respiratory syndrome (SARSCoV-
2) infection and COVID-19 mortality. However, information on the involvement of APOE4
in the underlying pathology and clinical symptoms is limited. Due to the high infection and mortality
rate of COVID-19 in AD individuals, challenges have been identified in the management of AD
patients during the COVID-19 pandemic. In order to provide evidence-based, more effective
healthcare, it is critical to identify underlying concerns and, preferably, biomarkers. The risk variant
APOE4 imparts enhanced infectivity by the underlying coronavirus SARS-CoV-2 at a cellular
level, genetic level, and route level. Here we review existing advances in clinical and basic research
on the AD-related gene APOE, as well as the role of APOE in AD pathogenesis, using neurobiological
evidence. Moreover, the role of APOE in severe COVID-19 in Alzheimer's patients
has also been reviewed.
Molecular Docking and Dynamics Study of Acetogenins Targeting Cyclin-dependent Kinase and In vitro Evaluation of Annona Muricata Fruit Extracts using MDA-MB-231 Breast Cancer Cell Line
Authors: Birendra Swapna , Kotha Satvik , Ramappa Harisha , Rao M. Raghavendra , Siddamsetty Ramachandra Setty 
Published: February 2023

Background:
Overexpressed CDK1 and CDK2 are targeted as potential sites for cancer treatment.
Annona muricata fruit has been reported to have more than 100 acetogenins showing cytotoxic
activities against cancer cell lines. Hence the study aims to demonstrate the cytotoxicity of ethyl acetate
fruit extract, its role in cell cycle progression, and apoptosis using the MDMBA-231 breast cancer cell
line. Docking, dynamics, and ADME studies were also demonstrated to generate lead molecules of AM
fruit responsible for cancer treatment.

Method:
Cell viability was quantified by the MTT assay. Cell cycle arrest and apoptotic cells were determined
by flow cytometry and PI annexin V-FITC staining by flow cytometry, respectively. Molecular
docking, molecular dynamics, and ADME properties of 11 acetogenins were studied using the schrödinger
maestro suite 2018-1.

Results:
The MTT assay revealed IC50 232.9μg/ml with a high degree of cytotoxicity. The extract effectively
caused cell cycle arrest at the G2M and S phases; early and late apoptosis was induced at 160 μg/ml
and 320 μg/ml. Docking scores of muricin L, J, and annomuricin A complexed with CDK2 and muricin J,
K, and L with CDK1 binding energy ranging is mentioned as a molecular dynamic study envisaged muricin
j against CDK2 stable hydrogen and hydrophobic interactions with critical residues like ASP-86,
GLN-131, HIS-84, LYS-89, PHE80, PHE82, and PHE83 throughout 200 ns (hinge region). ADMET
profiling also confirmed that all 11 ligands passed the rule of 5 and 3. The in vitro and in silico studies
revealed that these acetogenins could be CDK1 and CDK2 inhibitors for cancer treatment.

Conclusion:
The in vitro studies presume that the ethyl acetate fruit extract of AM is an excellent cytotoxic
agent. In silico studies demonstrated that muricin j could lead molecules to target kinase proteins
responsible for cell proliferation. ADME study enlightened us to take 11 acetogenins for the drug discovery
process in managing cancer treatment.
Using Cone Beam Computed Tomography for Radiological Assessment Beyond Dento-maxillofacial Imaging: A Review of the Clinical Applications in other Anatomical Districts
Authors: Corazza Ivan , Giannetti Emanuele , Bonzi Giancarlo , Lombi Alessandro , Paolani Giulia , Santoro Miriam , Morrone Maria Francesca , Zecchi Margherita , Rossi Pier Luca 
Published: February 2023

Background::Cone Beam Computed Tomography (CBCT) represents the optimal imaging solution for the evaluation of the maxillofacial and dental area when quantitative geometric and volumetric accuracy is necessary (e.g., in implantology and orthodontics). Moreover, in recent years, this technique has given excellent results for the imaging of lower and upper extremities. Therefore, significant interest has been increased in using CBCT to investigate larger and non-traditional anatomical districts.Objective::The purpose of this work is to review the scientific literature in Pubmed and Scopus on CBCT application beyond head districts by paying attention to image quality and radiological doses.Methods::The search for keywords was conducted in Pubmed and Scopus databases with no back-date restriction. Papers on applications of CBCT to head were excluded from the present work. From each considered paper, parameters related to image quality and radiological dose were extracted. An overall qualitative evaluation of the results extracted from each issue was done by comparing the conclusive remarks of each author regarding doses and image quality. PRISMA statements were followed during this process.Results::The review retrieved 97 issues from 83 extracted papers; 46 issues presented a comparison between CBCT and Multi-Detector Computed Tomography (MDCT), and 51 reviewed only CBCT. The radiological doses given to the patient with CBCT were considered acceptable in 91% of cases, and the final image quality was found in 99%.Conclusion::CBCT represents a promising technology not only for imaging of the head and upper and lower extremities but for all the orthopedic districts. Moreover, the application of CBCT derived from C-arms (without the possibility of a 360 ° rotation range) during invasive investigations demonstrates the feasibility of this technique for non-standard anatomical areas, from soft tissues to vascular beds, despite the limits due to the incomplete rotation of the tube.
Current Advances in Nanomaterial-associated Micro and Nano-devices for
SARS-CoV-2 Detection
Authors: Dhahi Thikra S. , Adam Tijjani , Gopinath Subash C.B. , Hashim Uda , Mohammed Aeshah M. , Mohammed Mohammed , Uda Muhammad N.A. 
Published: February 2023

Abstract:
COVID-19 spread rapidly around the world in 18 months, with various forms of variants
caused by severe acute respiratory syndrome (SARS-CoV). This has put pressure on the
world community and created an urgent need for understanding its early occurrence through
rapid, simple, cheap, and yet highly accurate diagnosis. The most widely adopted method as of
today is the real-time reverse-transcriptase polymerase chain reaction. This test has shown the
potential for rapid testing, but unfortunately, the test is not rapid and, in some cases, displays
false negatives or false positives. The nanomaterials play an important role in creating highly
sensitive systems, and have been thought to significantly improve the performance of the SARSCoV-
2 protocols. Several biosensors based on micro-and nano-sensors for SARS-CoV-2 detection
have been reported, and they employ multi-dimensional hybrids on sensing surfaces with
devices having different sizes and geometries. Zero-to-three-dimension nanomaterial hybrids on
sensing surfaces, including nanofilm hybrids for SARS-CoV-2 detection, were employed with
unprecedented sensitivity and accuracy. Furthermore, the sensors were nanofluidic and mediated
high-performance SARS-CoV-2 detection. This breakthrough has brought the possibility of
making a biosystem on a chip (Bio-SoC) for rapid, cheap, and point-of-care detection. This review
summarises various advancements in nanomaterial-associated nanodevices and metasurface
devices for detecting SARS-CoV-2.
Safety Profile of Molnupiravir with Significant Effect on COVID-19: A
Review
Authors: Mukherjee Tuhin , Mal Payel , Upadhyay Abhay Kumar , Mohanty Satyajit , Nayak Nikita , Singh Ravi Pratap , Pattnaik Ashok , Das Tanisha , Basak Sourav 
Published: February 2023

Background:
As the COVID era unfolds, researchers reveal that rapid changes in viral genetic
material allow viruses to circumvent challenges triggered by the host immune system and resist
anti-viral drugs, potentially leading to persistent viral manifestations in host cells. Molnupiravir
(RNA-dependent RNA polymerase inhibitor) is a novel anti-viral medicine promising a vital role in
coming setbacks.

Objectives::
This review aims to clarify the safety and efficacy of the molnupiravir molecule in light of
existing case studies. As a result, it is intended to explore and discuss the molecular structure, mechanism
of action, discovery and development process, preclinical research, clinical investigations, and
other subtopics.

Methods:
A total of 75 publications were searched using multiple engines, such as Google Scholar,
PubMed, Web of Science, Embase, Cochrane Library, ClinicalTrials.gov, and others, with a constraint
applied to exclude publications published over 11 years ago. Molnupiravir, safety, efficacy, COVID-
19, RdRp, PK-PD, and clinical study were utilized as keywords.

Result:
Clinical results on molnupiravir are supported by investigations that were recently disclosed
in a study on both sex volunteers (male and female) with an age restriction of 19 to 60 years, followed
by a Phase-3 Clinical Trial (NCT04575584) with 775 randomly assigned participants and no fatalities
reported due to treatment.

Conclusion:
Molnupiravir proved a high level of safety, allowing it to be tested further. This review
supports the safety and efficacy of this molecule based on the established evidence, which claims the
most anticipated employment of molnupiravir in COVID protocol.
MicroRNAs Improve Cancer Treatment Outcomes Through Personalized Medicine
Authors: Hatam Saeid 
Published: February 2023

Abstract:
MicroRNAs (miRNAs) are short non-coding RNAs that repress or degrade mRNA targets
to downregulate genes. In cancer occurrence, the expression of miRNAs is altered. Depending
on the involvement of a certain miRNA in the pathogenetic growth of a tumor, It may be up or
downregulated. The “oncogenic” action of miRNAs corresponds with upregulation, which leads to
tumor proliferation and spread meanwhile the miRNAs that have been downregulated bring tumorsuppressive
outcomes. Oncogenes and tumor suppressor genes are among the genes whose expression
is under their control, demonstrating that classifying them solely as oncogenes or tumor suppressor
genes alone is not only hindering but also incorrect. Apart from basic tumors, miRNAs may
be found in nearly all human fluids and can be used for cancer diagnosis as well as clinical outcome
prognostics and better response to treatment strategies. The overall variance of these tiny noncoding
RNAs influences patient-specific pharmacokinetics and pharmacodynamics of anti-cancer
medicines, driving a growing demand for personalized medicine. By now, microRNAs from tumor
biopsies or blood are being widely investigated as substantial biomarkers for cancer in time diagnosis,
prognosis, and, progression. With the rise of COVID-19, this paper also attempts to study recent
research on miRNAs involved with deaths in lung cancer COVID patients. With the discovery of
single nucleotide polymorphisms, personalized treatment via microRNAs has lately become a reality.
The present review article describes the highlights of recent knowledge of miRNAs in various
cancers, with a focus on miRNA translational applications as innovative potential diagnostic and
prognostic indicators that expand person-to-person therapy options.
Anticancer Potential of Biologically Active Diosgenin and its Derivatives:
An Update
Authors: Mondal Sadhan 
Published: February 2023

Abstract:
Diosgenin (1) is an important steroidal metabolite often used as a precursor material for
the synthesis of a broad array of steroidal drugs. Recent studies have indicated that compound (1)
and many diosgenyl derivatives such as (1→9) possess both in vivo and in vitro biological activities
like antioxidant, hypolipidemic, antithrombotic, immuno-modulatory, and estrogenic including anticancer
activities. This steroidal sapogenin is of increasing interest in the pharmaceutical industry
as it is being explored as an important starting material for the synthesis of oral contraceptives, sex
hormones, and many other steroidal drugs having anticancer potential. Naturally occurring diosgenin,
and its glucoside derivatives are quite abundant in several plant sources, but very little is known
about the biogenesis of this class of secondary plant metabolites. Hence, designing a semi-synthetic
protocol for the improved anticancer potential with enhanced efficacy of diosgenin and its derivatives
is always a challenging field of cutting-edge research in pharmaceutical chemistry. The present
review highlights some of the recent research findings and applications of this group of
triterpenoids as a potential anticancer agent.
Optimal Feature Selection from High-dimensional Microarray Dataset
Employing Hybrid IG-Jaya Model
Authors: Sahu Bibhuprasad , Dash Sujata 
Published: February 2023

Background:
Feature selection (FS) is a crucial strategy for dimensionality reduction
in data preprocessing since microarray data sets typically contain redundant and extraneous features
that degrade the performance and complexity of classification models.

Objective:
The purpose of feature selection is to reduce the number of features from highdimensional
cancer datasets and enhance classification accuracy.

Methods:
This research provides a wrapper-based hybrid model integrating information gain
(IG) and Jaya algorithm (JA) for determining the optimum featured genes from high-dimensional
microarray datasets. This paper's comprehensive study is divided into two segments: we employed
the parameterless JA to identify the featured gene subsets in the first stage without filter
methods. Various classifiers evaluate JA's performance, such as SVM, LDA, NB, and DT. In the
second section, we introduce a hybrid IG-JA model. The IG is used as a filter to eliminate redundant
and noisy features. The reduced feature subset is then given to the JA as a wrapper to improve
the hybrid model's performance using the classifiers outlined above.

Results:
We used 13 benchmark microarray data sets from the public repository for experimental
analysis. It is noteworthy to state that the hybrid IG-JA model performs better as compared to its
counterparts.

Conclusion:
Tests and statistics show that the suggested model outperforms the standard feature
selection method with JA and other existing models. Our proposed model is unable to provide the
best accuracy compared to other existing approaches; however, it is quite steady and good. In the
future, this work could be implemented with various filter methods and real-time data sets. A
multi-filter approach with the Jaya algorithm will be used to check the efficiency of the proposed
one. And it would be better to choose any other hybrid model (chaos-based) with Jaya to enhance
the feature selection accuracy with a high dimensional dataset.
Association of Uncontrolled Hypertension or Diabetes Mellitus With Major Adverse Cardiovascular Events and Mortality in South Korea: Population-Based Cohort Study
Authors: Oh Sung-Hee , Kim Dohyang , Hwang Jinseub , Kang Jae-Heon , Kwon Yeongkeun , Kwon Jin-Won 
Published: February 2023

Background
Managing hypertension (HT) and diabetes mellitus (DM) is crucial to preventing cardiovascular diseases. Few studies have investigated the incidence and risk of cardiovascular diseases or mortality in uncontrolled HT or DM in the Asian population. Epidemiological studies of cardiovascular disease should be conducted with continuous consideration of the changing disease risk profiles, lifestyles, and socioeconomic status over time.

Objective
We aimed to examine the association of uncontrolled HT or DM with the incidence of cardiovascular events or deaths from any cause.

Methods
This population-based retrospective study was conducted using data from the Korean National Health Insurance Service–National Health Screening Cohort, including patients aged 40-79 years who participated in national screening from 2002 to 2003 and were followed up until 2015. The health screening period from 2002 to 2013 was stratified into 6 index periods in 2-year cycles, and the follow-up period from 2004 to 2015 was stratified accordingly into 6 subsequent 2-year periods. The incidence rates and hazard ratio (HR) for major adverse cardiovascular events (MACE) and death from any cause were estimated according to HT or DM control status. Extended Cox models with time-dependent variables updated every 2 years, including sociodemographic characteristics, blood pressure (BP), fasting blood glucose (FBG), medication prescription, and adherence, were used.

Results
Among the total cohort of 440,249 patients, 155,765 (35.38%) were in the uncontrolled HT or DM group. More than 60% of the patients with HT or DM who were prescribed medications did not achieve the target BP or FBG. The incidence of MACE was 10.8-15.5 and 9.6-13.3 per 1000 person-years in the uncontrolled DM and uncontrolled HT groups, respectively, and increased with age. In the uncontrolled HT and DM group, the incidence of MACE was high (15.2-17.5 per 1000 person-years) at a relatively young age and showed no age-related trend. Adjusted HR for MACE were 1.28 (95% CI 1.23-1.32) for the uncontrolled DM group, 1.32 (95% CI 1.29-1.35) for the uncontrolled HT group, and 1.54 (95% CI 1.47-1.60) for the uncontrolled HT and DM group. Adjusted HR for death from any cause were 1.05 (95% CI 1.01-1.10) for the uncontrolled DM group, 1.13 (95% CI 1.10-1.16) for the uncontrolled HT group, and 1.17 (95% CI 1.12-1.23) for the uncontrolled HT and DM group.

Conclusions
This up-to-date evidence of cardiovascular epidemiology in South Korea serves as the basis for planning public health policies to prevent cardiovascular diseases. The high uncontrolled rates of HT or DM, regardless of medication prescription, have led us to suggest the need for a novel system for effective BP or glycemic control, such as a community-wide management program using mobile health technology.