bluuebunny/crossref_metadata_2025_split huggingface dataset:
SIFAT KUANTITATIF ENTOG (Cairina moschata) JANTAN YANG DIBERI RANSUM MENGANDUNG HIJAUAN ECENG GONDOK (Eicohornia crassipes)
Authors: Lindayani Olis , Dini Widianingrum  , Aaf Falahudin  
Published: January 2023

Karakteristik entog sangat penting dalam usaha pengembangan entog, meliputi sifat kuantitatif. Tujuan penelitian ini Menganalisis pengaruh penggunaan ransum mengandung hijauan eceng gondok terhadap sifat kuantitatif entog. Penelitian ini dilakuakan secara eksperimen dengan menggunakan disain Rancangan Rancangan Acak Lengkap (RAL). Sebanyak 80 ekor entog jantan ditempatkan pada 20 petak kandang., sehingga setiap petak kandangnya terdiri dari 4 ekor. Jenis perlakuan yang digunakan yaitu P0 (100% dedak padi) P1 (dedak padi 75% + 25% eceng gondok) P2 (dedak padi 50% + 50% eceng gondok) P3 (dedak padi 25% + 75% eceng gondok). Peubah yang diamati pada lebar paruh, panjang paruh, tinggi kepala, panjang kepala, panjang leher, panjang punggung, panjang sayap, panjang femur, panjang tibia, lebar dada, panjang badan, panjang jari ketiga. Data hasil penelitian sifat kualitatif dianalisis secara deskriptif sementara sifat kuantitatif dianalisis menggunakan Analisis Sidik Ragam atau Anova (Analisys Of Variance) dan diuji lanjut menggunakan Uji Jarak Berganda Duncan (Duncan Multiple Range Test). Hasil penelitian yang paling ideal pada panjang sayap 11,86, panjang tibia 10,96, panjang badan 7,70, lebar dada 7,36, panjang punggung. Kesimpulan menunjukan bahwa penggunaan ransum P2 50% dedak padi + 50% eceng gondok dapat memperoleh sifat kuantitaif entog yang baik.
THE OPPORTUNITY TO STUDY FOR STUDENTS IN THE REMOTE AREA OF SOUTHEAST ASIA AMID THE COVID-19 PANDEMICS. CASE STUDY: INTERNET CONNECTION AND ONLINE LEARNING IN MERAUKE PAPUA INDONESIA
Authors: Rudi Kogoya  
Published: January 2023

Abstract

This paper will discuss about challenges for students in the remote area of Indonesia. Students in Merauke will be the case study of this paper. Millions of people have suffered from job loss and wage cuts due to the economic shocks caused by Covid-19 pandemic. Not just in the economic terms the pandemics also affected education especially for the student in the remote area. By April 2020, 188 countries around the world had imposed nationwide school closures, affecting more than 1.5 billion children and youth. As a result, many schools and institutions are now offering distance learning to their pupils. Unfortunately, not everyone has the luxury to opt for this. We can imagine how will it be for the student that live in the rural area in Merauke Papua the eastern part of Indonesia for instance. They have to denied the physical distancing policy issued by the government with attending to school amid the deadly pandemic, because they have no choice. There is no adequate internet connection. This paper will used qualitative research and interview method to collect the data and answer the research question How Students in Merauke Papua Deal With the Online Learning Program? It is a huge challenge for both the government to provide best services and student to adapt.

Key Words: Covid-19, Indonesia, Internet, Students, Inequality.
Periodic health checkups reduce the risk of hospitalization in patients with type 2 diabetes
Authors: Hamasaki Hidetaka , Yanai Hidekatsu 
Published: January 2023

IntroductionPeriodic health checkups (PHCs) represent a unique system in Japan that is useful for the early detection of lifestyle-related diseases and cardiovascular diseases (CVDs). This study aims to investigate the association of PHCs with the hospitalization risk of patients with type 2 diabetes mellitus (T2DM).MethodsA retrospective cohort study was conducted from April 2013 to December 2015 and included participant information such as CVD history, lifestyle, and whether PHC was conducted in addition to regular medical examinations. Difference in clinical data between patients with and without PHC was examined. Furthermore, Cox regression analysis was performed to investigate the independent association of PHCs with hospitalization.ResultsHerein, 1,256 patients were selected and followed up for 2.35 ± 0.73 years. In the PHC group, body mass index, waist circumference, proportion of patients with a history of CVD, and number of hospitalizations were lower than those in the non-PHC group. Furthermore, the PHC group exhibited a significant association with lower hospitalization risk (hazard ratio = 0.825; 95% confidence interval, 0.684 to 0.997; p = 0.046) in the Cox model.ConclusionThis study revealed that PHCs minimized the risk of hospitalization in patients with T2DM. Furthermore, we discussed the effectiveness of PHCs in enhancing health outcomes and reducing health care costs in such patients.
Endothelial ADAM10 controls cellular response to oxLDL and its deficiency exacerbates atherosclerosis with intraplaque hemorrhage and neovascularization in mice
Authors: van der Vorst Emiel P. C. , Maas Sanne L. , Theodorou Kosta , Peters Linsey J. F. , Jin Han , Rademakers Timo , Gijbels Marion J. , Rousch Mat , Jansen Yvonne , Weber Christian , Lehrke Michael , Lebherz Corinna , Yildiz Daniela , Ludwig Andreas , Bentzon Jacob F. , Biessen Erik A. L. , Donners Marjo M. P. C. 
Published: January 2023

IntroductionThe transmembrane protease A Disintegrin And Metalloproteinase 10 (ADAM10) displays a “pattern regulatory function,” by cleaving a range of membrane-bound proteins. In endothelium, it regulates barrier function, leukocyte recruitment and angiogenesis. Previously, we showed that ADAM10 is expressed in human atherosclerotic plaques and associated with neovascularization. In this study, we aimed to determine the causal relevance of endothelial ADAM10 in murine atherosclerosis development in vivo.Methods and resultsEndothelial Adam10 deficiency (Adam10ecko) in Western-type diet (WTD) fed mice rendered atherogenic by adeno-associated virus-mediated PCSK9 overexpression showed markedly increased atherosclerotic lesion formation. Additionally, Adam10 deficiency was associated with an increased necrotic core and concomitant reduction in plaque macrophage content. Strikingly, while intraplaque hemorrhage and neovascularization are rarely observed in aortic roots of atherosclerotic mice after 12 weeks of WTD feeding, a majority of plaques in both brachiocephalic artery and aortic root of Adam10ecko mice contained these features, suggestive of major plaque destabilization. In vitro, ADAM10 knockdown in human coronary artery endothelial cells (HCAECs) blunted the shedding of lectin-like oxidized LDL (oxLDL) receptor-1 (LOX-1) and increased endothelial inflammatory responses to oxLDL as witnessed by upregulated ICAM-1, VCAM-1, CCL5, and CXCL1 expression (which was diminished when LOX-1 was silenced) as well as activation of pro-inflammatory signaling pathways. LOX-1 shedding appeared also reduced in vivo, as soluble LOX-1 levels in plasma of Adam10ecko mice was significantly reduced compared to wildtypes.DiscussionCollectively, these results demonstrate that endothelial ADAM10 is atheroprotective, most likely by limiting oxLDL-induced inflammation besides its known role in pathological neovascularization. Our findings create novel opportunities to develop therapeutics targeting atherosclerotic plaque progression and stability, but at the same time warrant caution when considering to use ADAM10 inhibitors for therapy in other diseases.
The management correlation between metabolic index, cardiovascular health, and diabetes combined with cardiovascular disease
Authors: Zhang Yi , Liu Chao , Xu Yijing , Wang Yanlei , Dai Fang , Hu Honglin , Jiang Tian , Lu Yunxia , Zhang Qiu 
Published: January 2023

BackgroundCardiovascular disease (CVD) has become a major cause of morbidity and mortality in patients with type 2 diabetes mellitus (T2DM). Although there is also evidence that multifactorial interventions to control blood glucose, blood pressure, and lipid profiles can reduce macrovascular complications and mortality in patients with T2DM, the link between these risk factors has not been established.MethodsOn 10 December 2018, 1,920 people in four cities in Anhui Province were included. Latent category analysis (LCA) was used to explore the clustering mode of HRBs (health risk behaviors). The primary exposure was HRBs and exercise and diet interventions, and the primary outcome was CVD and other variables, including zMS, triglyceride-glucose index (TyG), TyG-WC (waist circumference), TyG-BMI, TG/HDL, and cardiovascular health (CVH). A multivariable logistic regression model was used to establish the relationship between HRBs, exercise, diet interventions, and CVD. Moderate analysis and mediation moderation analysis were employed by the PROCESS method to explore the relationship between these variables. Sensitivity analysis explored the robustness of the model.ResultsThe mean age was 57.10 ± 10.0 years old. Overall, CVD affects approximately 19.9% of all persons with T2DM. Macrovascular complications of T2DM include coronary heart disease, myocardial infarction (MI), cardiac insufficiency, and cerebrovascular disease. Elderly age (χ2 = 22.70), no occupation (χ2 = 20.97), medium and high socioeconomic status (SES) (χ2 = 19.92), higher level of TyG-WC (χ2 = 6.60), and higher zMS (χ2 = 7.59) were correlated with high CVD. Many metabolic indices have shown a connection with T2DM combined with CVD, and there was a dose−response relationship between HRB co-occurrence and clustering of HRBs and zMS; there was a dose−response relationship between multifactorial intervention and CVH. In the mediation moderation analysis, there was an association between HRB, gender, TyG, TyG-BMI, and CVD. From an intervention management perspective, exercise and no diet intervention were more significant with CVD; moreover, there was an association between intervention management, gender, zMS, TyG-WC, TyG-BMI, TG/HDL, and CVD. Finally, there was an association between sex, CVH, and CVD. Sensitivity analysis demonstrated that our results were robust.ConclusionsCVD is one of the common complications in patients with type 2 diabetes, and its long-term outcome will have more or less impact on patients. Our findings suggest the potential benefits of scaling up multifactorial and multifaceted interventions to prevent CVD in patients with T2DM.
Investigation of He’s Yang Chao recipe against oxidative stress-related mitophagy and pyroptosis to improve ovarian function
Authors: Miao Chenyun , Zhao Ying , Chen Yun , Wang Ruye , Ren Ning , Chen Bixia , Dong Pingpei , Zhang Qin 
Published: January 2023

BackgroundPrimary ovarian insufficiency (POI) is a common gynecological disease with serious ramifications including low pregnancy rate and low estrogen symptoms. Traditional Chinese medicine is regarded as an effective treatment for POI. However, the therapeutic mechanism of it is unclear.MethodsIn this study, a mouse model of primary ovarian insufficiency was established by intraperitoneal injection of cyclophosphamide (CTX) and He’s Yang Chao Recipe (HSYC) concentrate was used for intragastric administration. Serum hormone levels (Anti-Müllerian Hormone, Estradiol, Progesterone, Luteinizing Hormone and Follicle Stimulating Hormone) and Oxidative Stress (OS) related products, superoxide dismutase (SOD), GSH-Px, and malondialdehyde (MDA) were measured by enzyme-linked immunosorbent assay. Pathological changes in ovarian tissue were evaluated by hematoxylin and eosin staining, and flow cytometry was used to determine reactive oxygen species content and mitochondrial membrane potential levels in granulosa cells. Mitochondrial distribution and morphology were investigated using immunofluorescence staining. The level of mitophagy was evaluated by LC3 immunofluorescence staining and autophagosome counts using electron microscopy. Western blotting and qPCR were used to detect the expression of proteins and genes related to mitophagy and the NLRP3 inflammasome.ResultsAfter HSYC treatment, the ovarian damage was milder than in the CTX group. Compared with the CTX group; SOD, GSH-Px, and the total antioxidant capacity were significantly increased, while MDA and ROS were decreased in the HSYC treatment groups. Furthermore, mitochondrial distribution and membrane potential levels were improved after HSYC treatment compared to the CTX group. After the HSYC treatment, the LC3 fluorescent intensity and autophagosome counts were decreased. Similarly, mitophagy related markers PINK1, Parkin, LC3, and Beclin1 were decreased, while p62 was significantly increased, compared with the CTX groups. The mRNA and protein expression of NLRP3 inflammasome, NLRP3, caspase-1, GSDMD, IL-18, and IL-1β were significantly decreased in the HSYC treatment groups.ConclusionThis is the first study in molecular mechanisms underlying HSYC against granulosa cell injury in POI. HSYC protects ovaries from CTX-induced ovarian damage and oxidative stress. HSYC enhanced ovarian function in mice with primary ovarian insufficiency by inhibiting PINK1-Parkin mitophagy and NLRP3 inflammasome activation.
Psychological factors in functional hypothalamic amenorrhea: A systematic review and meta-analysis
Authors: Bonazza Federica , Politi Giuliana , Leone Daniela , Vegni Elena , Borghi Lidia 
Published: January 2023

BackgroundPsychological factors have been found to be associated with functional hypothalamic amenorrhea (FHA); however, their role in the onset or persistence of FHA is still understudied. The study aims to assess the associations of psychological factors with the presence vs the absence of FHA.MethodsA systematic literature search has been conducted across the major databases (PubMed, PsycINFO, Scopus, and Embase) to explore the psychological factors associated with FHA. The search was limited to English-written articles published from 2000 onwards. Articles were selected based on stringent inclusion/exclusion criteria. After data extraction, meta-analysis and meta-synthesis were conducted.ResultsOf 349 retrieved articles, eight studies were included. Findings indicate that the main psychological factors associated to FHA seem to be depression and eating attitudes, especially drive for thinness. FHA women present higher levels of anxiety, sleep disorders, dysfunctional attitudes, and alexithymia. The meta-analysis on drive for thinness revealed that the pooled MD across the studies was statistically significant both in the fixed 0.63 (95% CI: 0.31–0.95) and random model 0.70 (95% CI: 0.13–1.26). Likewise, as for depression, the pooled MD across the studies was statistically significant both in the fixed 0.60 (95% CI: 0.36–0.84) and random model 0.61 (95% CI: 0.20–1.01).DiscussionFindings showed the association of psychological factors and FHA and recognized their involvement in the persistence of the disorder. A multidisciplinary approach should involve a collaborative process between gynecologists, clinical psychologists, and psychiatrists, from diagnosis to treatment. Longitudinal studies should be implemented with a comparison/control group or by including clinical psychologists in the psychological assessment and study design.
Tween 20 Stabilized Conventional Heavy Crude Oil-In-Water Emulsions Formed by Mechanical Homogenization
Authors: Shen Wanhua , Koirala Narayan , Mukherjee Debjani , Lee Kenneth , Zhao Min , Li Jianbing 
Published: January 2023

This study investigated the preparation of stable conventional heavy crude oil-in-water (O/W) emulsions by mechanical homogenization with the addition of a non-ionic surfactant, Tween-20. A four-factor, five-level central composite design was carried out to investigate the effects of four independent variables, including mixing intensity (4,000–10,000 rpm), mixing duration (5–45 min), water salinity (0–40 g/L), and the concentration of emulsifier (0.1–2.1 wt%) on the emulsion stability. Emulsion stability was determined by quantification of creaming index, turbidity change rate, and average oil droplet size. The results demonstrated that the salinity of 30 g/L, mixing intensity of 8,500 rpm, mixing duration of 35 min, and emulsifier concentration of 1.6 wt% led to the formation of the most stable emulsion.
Clinical and genetic features of luscan-lumish syndrome associated with a novel de novo variant of SETD2 gene: Case report and literature review
Authors: Zhang Yanqing , Zhang Haozheng , Wu Wei , Wang Dong , Lv Yuqiang , Zhao Dongmei , Wang Lingxiao , Liu Yi , Zhang Kaihui 
Published: January 2023

Introduction: Luscan-Lumish syndrome (LLS) is currently recognized as a rarely-observed condition featured with overgrowth, macrocephaly, obesity, type I Chiari malformation, and linguistic retardation. So far, there have been only a few LLS cases registered worldwide, but with none of them reported from China. To acquire a deeper understanding on the clinical and genetic features of this disease, a Chinese boy with LLS caused by a heterozygous variant in SETD2 gene was investigated in the present study.Methods: The patient was clinically examined and the medical history of his family was collected. Genetic testing was performed to determine the genetic etiology.Results: The proband was a boy aged 5-year-7-month-old, who was referred to our hospital due to “being a slow learner in kindergarten”. The child had a history of delayed motor and language development in comparison to his peers. After admission, physical examination revealed tall stature and macrocephaly as the major manifestation, in addition to a relatively lower rating in intelligence assessment as well as abnormal MRI images showing a slightly shorter corpus callosum accompanied by a mildly thinner corpus callosum body. Whole exome sequencing (WES) revealed a heterozygous c.2514_2516delTAG (p.Ser838del) variant in SETD2 gene, which was subsequently identified as a novel de novo variant. According to the standardized genetic variant classification published by the American College of Medical Genetics and Genomics (ACMG), the variant, with a pathogenicity analysis result indicating PS2 + PM2_Supporting + PM4, was determined to be likely pathogenic. Through literature review, the clinical phenotypes of the 15 LLS cases were summarized, including 8 cases of overgrowth (53%), 13 cases of macrocephaly (87%), 11 cases of developmental delay (73%), 8 cases of autism (53%), and 7 cases of special facial features (47%). Besides, abnormal craniocerebral MRI findings were noticed in 7 cases. Despite that the mutation sites of the 15 patients varied from case to case, they showed a uniformly distributed pattern throughout the whole SETD2 gene, including 5 missense mutations, 5 frameshift mutations and 5 non-sense mutations.Conclusion: LLS, not having been recognized till recent years, is identified as an autosomal dominant syndrome triggered by SETD2 gene mutation. As the first report of LLS in China, the case in our study was proved to be associated with a unique type of SETD2 gene mutation that has never been reported previously, which is believed to enrich the mutation spectrum of SETD2 gene and also, deepening the clinicians’ understanding on the disease.
Immunogenicity, Effectiveness, and Safety of Inactivated Virus (CoronaVac) Vaccine in a Two-Dose Primary Protocol and BNT162b2 Heterologous Booster in Brazil (Immunita-001): A One Year Period Follow Up Phase 4 Study
Authors: Grenfell Rafaella F. Q. , Almeida Nathalie B. F. , Filgueiras Priscilla S. , Corsini Camila A. , Gomes Sarah V. C. , de Miranda Daniel A. P. , Lourenço Adelina J. , Martins-Filho Olindo A. , de Oliveira Jaquelline G. , Teixeira-Carvalho Andrea , Campos Guilherme R. F. , Nogueira Mauricio L. , Alves Pedro Augusto , Fernandes Gabriel R. , Castilho Leda R. , Lima Tulio M. , de Abreu Daniel P. B. , Alvim Renata G. F. , Silva Thaís Bárbara de S. , Jeremias Wander de J. , Otta Dayane A. , Campi-Azevedo Ana Carolina ,   Immunita-001 Team
Published: January 2023

BackgroundEffective and safe vaccines against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) are critical to controlling the COVID-19 pandemic and will remain the most important tool in limiting the spread of the virus long after the pandemic is over.MethodsWe bring pioneering contributions on the maintenance of the immune response over a year on a real-life basis study in 1,587 individuals (18-90 yrs, median 39 yrs; 1,208 female/379 male) who underwent vaccination with two doses of CoronaVac and BNT162b2 booster after 6-months of primary protocol.FindingsElevated levels of anti-spike IgG antibodies were detected after CoronaVac vaccination, which significantly decreased after 80 days and remained stable until the introduction of the booster dose. Heterologous booster restored antibody titers up to-1·7-fold, changing overall seropositivity to 96%. Titers of neutralising antibodies to the Omicron variant were lower in all timepoints than those against Delta variant. Individuals presenting neutralising antibodies against Omicron also presented the highest titers against Delta and anti-Spike IgG. Cellular immune response measurement pointed out a mixed immune profile with a robust release of chemokines, cytokines, and growth factors on the first month after CoronaVac vaccination followed by a gradual reduction over time and no increase after the booster dose. A stronger interaction between those mediators was noted over time. Prior exposure to the virus leaded to a more robust cellular immune response and a rise in antibody levels 60 days post CoronaVac than in individuals with no previous COVID-19. Both vaccines were safe and well tolerated among individuals.InterpretationOur data approach the effectiveness of CoronaVac association with BNT162b2 from the clinical and biological perspectives, aspects that have important implications for informing decisions about vaccine boosters.FundingFiocruz, Brazil.
Meta-analysis of neoadjuvant immunotherapy for non-metastatic colorectal cancer
Authors: Zhou Long , Yang Xiao-Quan , Zhao Guang-yue , Wang Feng-jian , Liu Xin 
Published: January 2023

BackgroundImmunotherapy has been approved for the treatment of metastatic colorectal cancer. The efficacy and safety of neoadjuvant immunotherapy for the treatment of non-metastatic colorectal cancer remains unclear. We tried to explore clinical effect of neoadjuvant immunotherapy in the treatment of non-metastatic colorectal cancer.MethodsWe searched the databases (PubMed, Wanfang Embase, Cochrane Library and China National Knowledge Infrastructure databases) to obtain suitable articles up to September 2022. The primary outcomes of pathological complete response (pCRs), major pathological response (MPR), objective response rate (ORR), R0-resection and anus preserving rate were collected and evaluated. Secordary outcomes (pCRs and MPR) of subgroup analysis between deficient mismatch repair/microsatellite instability-high group (dMMR/MSI-H) and proficient mismatch repair/microsatellite stable group (pMMR/MSS) and outcomes for rectal cancer were analyzed for the final results.ResultsWe included ten articles and 410 cases of non-metastatic colorectal cancer with neoadjuvant immunotherapy. There were 113 (27.5%) cases with the dMMR/MSI-H status and 167 (40.7%) cases with the pMMR/MSS status. pCRs was found in 167/373 (44.6%) patients (ES: 0.49, 95% CI: 0.36 to 0.62, P&lt;0.01, chi2 = 65.3, P&lt;0.01, I2 = 86.2%) and MPR was found in 194/304 (63.8%) patients (ES: 0.66, 95% CI: 0.54 to 0.78, P&lt;0.01, chi2 = 42.55, P&lt;0.01, I2 = 81.2%) with the random-effects model and huge heterogeneity. In the subgroup analysis, pCRs was higher in the dMMR/MSI-H group than the pMMR/MSS group in the fixed-effects model with minimal heterogeneity (OR: 3.55, 95% CI: 1.74 to 7.27, P&lt;0.01, chi2 = 1.86, P=0.6, I2 = 0%). pCRs was found in 58/172 (33.9%) rectal cancer patients (ES: 0.33, 95% CI: 0.26 to 0.40, P&lt;0.01, chi2 = 3.04, P=0.55, I2 = 0%) with the fixed-effects model and little heterogeneity.ConclusionNeoadjuvant immunotherapy could increase pCRs and MPR rate for non-metastatic colorectal cancer. Neoadjuvant immunotherapy could achieve better pCRs rate in dMMR/MSI-H group than in the pMMR/MSS group. Neoadjuvant immunotherapy could be another treatment option for non-metastatic colorectal cancer.Systematic review registrationhttps://www.crd.york.ac.uk/prospero/#myprospero, identifier CRD42022350523.