bluuebunny/crossref_metadata_2025_split huggingface dataset:
Identification of Biomarkers Related to Liquid-Liquid Phase Separation for Ulcerative Colitis Based on Single-Cell and Bulk RNA Transcriptome Sequencing Data
Authors: Lu Jicheng , Lu Xu , Chen Bin 
Published: January 2025

Background:
Liquid-Liquid Phase Separation (LLPS) is a process involved in the formation
of established organelles and various condensates that lack membranes; however, the relationship
between LLPS and Ulcerative Colitis (UC) remains unclear.

Aims:
This study aimed to comprehensively clarify the correlation between ulcerative colitis (UC)
and liquid-liquid phase separation (LLPS).

Objectives:
In this study, bioinformatics analyses and public databases were applied to screen and
validate key genes associated with LLPS in UC. Furthermore, the roles of these key genes in UC
were comprehensively analyzed.

Methods:
Based on the single-cell transcriptomic data of UC obtained from the Gene Expression
Omnibus (GEO) database, differences between patients with UC and their controls were compared
using the limma package. The single-cell data were then filtered and normalized by the ‘Seurat’
package and subjected to dimension reduction by the Uniform Manifold Approximation and
Projection (UMAP) algorithm. The LLPS-related genes (LLPSRGs) were searched on the Dr-
LLPS website to obtain cross-correlated genes, which were scored using the ssGSEA algorithm.
Next, functional enrichment, interaction network, immune landscape, and diagnostic and drug prediction
of the LLPSRGs were comprehensively explored. Finally, the results were validated using
external datasets and quantitative real-time PCR (qRT-PCR).

Results:
A total of eight cell types in UC were classified, namely, fibroblasts, macrophages, endothelial
cells, neutrophils, NK cells, B cells, epithelial cells, and T cells. The intersection between
differently expressed genes (DEGs) among the eight cell types identified 44 key genes,
which were predominantly enriched in immune- and infection-related pathways. According to receiver
operating characteristic (ROC) curves, PLA2G2A, GZMK, CD69, HSP90B1, and S100A11
reached an AUC value of 0.94, 0.95, 0.86, 0.89, and 0.93, respectively. Drug prediction revealed
that decitabine, tetrachlorodibenzodioxin, tetradecanoylphorbol acetate, thapsigargin, and cisplatin
were the potential small molecular compounds for PLA2G2A, GZMK, CD69, HSP90B1, and
S100A11. Immune cell infiltration analysis demonstrated that the infiltration of CD4 memory T
cell activation, macrophage M1, T macrophage M0, neutrophils, and mast cell activation was higher
in the UC group than in the normal group

Conclusion:
The LLPSRGs play crucial roles in UC and can be used as prognostic and diagnostic
markers for UC. The current findings contribute to the management of UC.
Investigating the Effect of Studying in the Home City on Quality of Life, Academic Burnout, Anxiety, and Depression Levels among Medical Students of Athens and Patras
Authors: Chalakatevaki Engeliki-Evelpia , Vlastos Dimitris D. , Theofilou Paraskevi 
Published: January 2025

Introduction:
Increased levels of stress, pressure, and possible isolation due to
intense study results in academic burnout, increased levels of anxiety, depressive feelings,
and a pessimistic outlook on life.

Purpose:
The purpose of the study was to investigate the levels of anxiety, depression,
academic burnout and quality of life in medical students of the University of Athens and
Patras, with the influence factor of studying in the cities of origin.

Methodology:
The sample, selected using a convenience sampling method, consisted of
159 medical students from two universities representing all years. Specifically, 41 men and
118 women participated in the survey, corresponding to 26%, 74%, and 1%, respectively.
The data collection tool was a four-part questionnaire: first, the collection of demographic
characteristics, second, the Depression Anxiety and Stress Scale questionnaire (DASS-21),
third, the Quality-of-Life Scale (QOLS), and fourth, the Copenhagen Burnout Scale for
Students (CBI-S). All questionnaires were used in their translated form. Statistical analysis
was performed using the non-parametric Mann-Whitney U test.

Results:
Our initial assumption that there would be statistically significant differences between
genders, cities of study (native or not), and schools proved to be wrong. Therefore,
no differences were found between genders, cities of study, or universities in terms of anxiety,
depression, and academic burnout. However, a statistically significant difference was
found between the university of study and the quality of life of the students. Thus, the hypothesis
that there would be a difference between universities (EKPA or Patras) and the
students' quality of life was confirmed.

Conclusion:
There were statistically significant results between the QOLS total and the
independent variable of the university. The use of a larger and more evenly distributed
sample is recommended.

other:
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jornal impresso e a abordagem sobre confeitaria na década de 1880
Authors: Firmino da Silva Maria Thaís 
Published: January 2025

Este artigo tem como premissa apresentar análise no âmbito da relação mídia-gastronomia sob a necessidade de contribuir com abordagens que privilegiam o resgate histórico desse entrecruzamento. Para tanto, a partir de recorte sob o crivo da temática confeitaria e doçaria, foi viabilizada investigação em edições do período de 1880 a 1889 do jornal impresso brasileiro Gazeta do Norte: Orgão Liberal, especificamente na editoria denominada Conhecimentos Úteis. O estudo se respaldou em abordagem qualitativa e quantitativa, com método próprio amparado pela análise de conteúdo, e primou pela execução das seguintes etapas: 1) Aprofundamento temático e contextual; 2) Exploração do material; 3) Coleta de dados; 4) Análise de dados; 5) Apresentação de resultados.
Strategies to Increase Response Rate and Reduce Nonresponse Bias in Population Health Research: Analysis of a Series of Randomized Controlled Experiments during a Large COVID-19 Study
Authors: Atchison Christina J , Gilby Nicholas , Pantelidou Galini , Clemens Sam , Pickering Kevin , Chadeau-Hyam Marc , Ashby Deborah , Barclay Wendy S , Cooke Graham S , Darzi Ara , Riley Steven , Donnelly Christl A , Ward Helen , Elliott Paul 
Published: January 2025

Abstract

Background
High response rates are needed in population-based studies, as nonresponse reduces effective sample size and bias affects accuracy and decreases the generalizability of the study findings.

Objective
We tested different strategies to improve response rate and reduce nonresponse bias in a national population–based COVID-19 surveillance program in England, United Kingdom.

Methods
Over 19 rounds, a random sample of individuals aged 5 years and older from the general population in England were invited by mail to complete a web-based questionnaire and return a swab for SARS-CoV-2 testing. We carried out several nested randomized controlled experiments to measure the impact on response rates of different interventions, including (1) variations in invitation and reminder letters and SMS text messages and (2) the offer of a conditional monetary incentive to return a swab, reporting absolute changes in response and relative response rate (95% CIs).

Results
Monetary incentives increased the response rate (completed swabs returned as a proportion of the number of individuals invited) across all age groups, sex at birth, and area deprivation with the biggest increase among the lowest responders, namely teenagers and young adults and those living in more deprived areas. With no monetary incentive, the response rate was 3.4% in participants aged 18‐22 years, increasing to 8.1% with a £10 (US $12.5) incentive, 11.9% with £20 (US $25.0), and 18.2% with £30 (US $37.5) (relative response rate 2.4 [95% CI 2.0-2.9], 3.5 [95% CI 3.0-4.2], and 5.4 [95% CI 4.4-6.7], respectively). Nonmonetary strategies had a modest, if any, impact on response rate. The largest effect was observed for sending an additional swab reminder (SMS text message or email). For example, those receiving an additional SMS text message were more likely to return a completed swab compared to those receiving the standard email-SMS approach, 73.3% versus 70.2%: percentage difference 3.1% (95% CI 2.2%-4.0%).

Conclusions
Conditional monetary incentives improved response rates to a web-based survey, which required the return of a swab test, particularly for younger age groups. Used in a selective way, incentives may be an effective strategy for improving sample response and representativeness in population-based studies.
Longitudinal Changes in Pitch-Related Acoustic Characteristics of the Voice Throughout the Menstrual Cycle: Observational Study
Authors: Kaufman Jaycee , Jeon Jouhyun , Oreskovic Jessica , Thommandram Anirudh , Fossat Yan 
Published: January 2025

Abstract

Background
Identifying subtle changes in the menstrual cycle is crucial for effective fertility tracking and understanding reproductive health.

Objective
The aim of the study is to explore how fundamental frequency features vary between menstrual phases using daily voice recordings.

Methods
This study analyzed smartphone-collected voice recordings from 16 naturally cycling female participants, collected every day for 1 full menstrual cycle. Fundamental frequency features (mean, SD, 5th percentile, and 95th percentile) were extracted from each voice recording. Ovulation was estimated using luteinizing hormone urine tests taken every morning. The analysis included comparisons of these features between the follicular and luteal phases and the application of changepoint detection algorithms to assess changes and pinpoint the day in which the shifts in vocal pitch occur.

Results
The fundamental frequency SD was 9.0% (SD 2.9%) lower in the luteal phase compared to the follicular phase (95% CI 3.4%‐14.7%; P=.002), and the 5th percentile of the fundamental frequency was 8.8% (SD 3.6%) higher (95% CI 1.7%‐16.0%; P=.01). No significant differences were found between phases in mean fundamental frequency or the 95th percentile of the fundamental frequency (P=.65 and P=.07). Changepoint detection, applied separately to each feature, identified the point in time when vocal frequency behaviors shifted. For the fundamental frequency SD and 5th percentile, 81% (n=13) of participants exhibited shifts within the fertile window (P=.03). In comparison, only 63% (n=10; P=.24) and 50% (n=8; P=.50) of participants had shifts in the fertile window for the mean and 95th percentile of the fundamental frequency, respectively.

Conclusions
These findings indicate that subtle variations in vocal pitch may reflect changes associated with the menstrual cycle, suggesting the potential for developing a noninvasive and convenient method for monitoring reproductive health. Changepoint detection may provide a promising avenue for future work in longitudinal fertility analysis.
Building a 3D Model in the Time-Domain of the Vertical-Vertical Controlled-Source Electromagnetic Method
Authors: Souza Danusa Mayara , Silva Marcos Welby Correa , Tocantins Victor Cezar 
Published: January 2025

The exploratory activity involves several uncontrollable factors, such as the presence or absence of oil and the quality of the extracted product. To minimize uncertainties and ambiguities encountered during oil and gas prospecting, controlled-source electromagnetic methods in the marine environment have gained prominence owing to technological and operational innovations. Studies have shown that the Marine Controlled-Source Electromagnetic (MCSEM) method is a suitable and sustainable for directly indicating hydrocarbons. In order to optimize MCSEM further, the source-receiver configuration as well as its reading mode were changed, creating the Vertical-Vertical Controlled-Source Electromagnetic (VVCSEM) method. We tested the source and receiver arrangements, modeled in a multiphysics modeler the VVCSEM method, and analyzed the promise which was developed to have a higher vertical resolution and promoting a clear distinction between conductive and resistive bodies. In this manuscript, we suggested constructing the modeling of the VVCSEM in steps. There are two steps for the modeling: the source’s response (switch-on) in the stationary regime and recording of the electromagnetic field interaction by the receivers in the transient regime. As reported previously in the benchmark literature, the VVCSEM effectively detected the suggested resistive anomalies.
Correction of chronic tonsillitis as a component of pre-pregnancy preparation for primary miscarriage
Authors: Filipeva P. V. , Svistushkin V. M. , Kiryushchenkov P. A. , Startseva N. M. , Zolotova A. V. , Devyatova E. A. , Loginova E. V. 
Published: January 2025

Aim. To substantiate the effectiveness of conservative treatment of CT at the pre-pregnancy stage in order to reduce reproductive losses.Materials and Methods. The study involved 86 women aged 21−35 years (on average 29.4±4.6 years) with a history of habitual miscarriage and chronic tonsillitis. The main group (n = 45) consisted of patients who were treated for chronic tonsillitis at the pre-gravidar stage. The comparison group (n = 41) consisted of patients who did not receive treatment for chronic tonsillitis at the pre-gravidar stage. The parameters of hemostasiogram and uterine blood flow in dynamics in patients of the main group were analyzed.Results. Against the background of chronic tonsillitis and habitual miscarriage, all parts of hemostasis are disrupted and uterine blood flow worsens. When studying the effect of circulating RFMC, it was found that their presence increases the risk of abnormal fibrinogen concentrations (OR = 1.95; 95 % CI 0.46– 8.21) and PTI (OR = 1.35; 95 % CI 0.31–5.88), increases the frequency of abnormal INR values (OR = 1.55; 95 % CI 0.42–5.65). At the same time, circulating RFMC does not affect the risk of violation of the duration of ACTV. The presence of RFMC does not significantly affect the dynamics of hemostasiogram indicators before and after treatment of chronic tonsillitis, whereas patients with negative RFMC experience significant changes in hemostasiogram indicators before and after treatment of chronic tonsillitis.After treatment of chronic tonsillitis, no patient showed a decrease in endometrial thickness. The blood flow rate in the PMA and LMA returned to normal, whereas before treatment, blood flow in the PMA was reduced in 1 (2.2 %), in the LMA was reduced in 7 (15.6 %) patients and increased in 2 (4.4 %) women. The resistance index in PMA and LMA returned to normal in all patients. The index of resistance of the arcuate arteries after treatment − increased values remained unchanged in 2 (4.4 %) patients.Conclusion. The results obtained in our study prove the negative effect of chronic tonsillitis on the hemostasis system and, as a result, deterioration of uterine blood flow and substantiate the effectiveness of conservative treatment of chronic tonsillitis at the pre-gravidar stage in order to reduce reproductive failures.
Meningkatkan Kemampuan Analisis Peserta Didik pada Materi Sistem Saraf melalui Pembelajaran Blended Learning berbasis TPACK
Authors: Rosalina Leni Rosalina , Nia Nurdiani  , Cartono  
Published: January 2025

Kemampuan analisis merupakan kemampuan dalam memecahkan permasalahan dalam pembelajaran, dengan menguraikannya menjadi unsur-unsur serta menentukan keterkaitan antar unsur-unsur tersebut dalam struktur keseluruhan. Kemampuan analisis penting dimiliki peserta didik khususnya dalam pelajaran biologi yang sarat dengan konsep-konsep sistem yang abstrak. Penelitian ini bertujuan untuk mengetahui adakah peningkatan kemampuan analisis peserta didik pada pokok materi sistem saraf dengan pembelajaran blended learning berbasis TPACK. Penelitian ini menggunakan metode eksperimen dengan rancangan pre-experimental design berbentuk One-Group Pretest-Posttest Design. Populasi dalam penelitian ini adalah peserta didik kelas XI MIPA SMA Angkasa Lanud Husein Sastranegara Bandung dengan sampel kelas XI MIPA A yang dicuplik secara purposive. Hasil penelitian menunjukkan bahwa pembelajaran dengan pendekatan blended learning berbasis TPACK dapat meningkatkan kemampuan analisis peserta didik pada materi pokok sistem saraf (Gain rata-rata 34,00) dengan dominasi kategori peningkatan tinggi (84 % peserta didik) (0,73 ≤ N-Gain ≤ 1,00). Berdasarkan hasil penelitian ini disarankan bagi peneliti selanjutnya untuk menguasai kerangka pembelajaran TPACK agar lebih mudah penerapannya dalam pembelajaran.
Pengembangan Asesmen Autentik Dengan Menggunakan Metode Jigsaw Untuk Mengukur Kemampuan Kolaboratif Dan Berpikir Kritis Mahasiswa Pendidikan Biologi
Authors: Eliaumra  , Sumarno A.Hulinggi  , Dewi Purwasih Samaela  
Published: January 2025

Tujuan utama pembelajaran abad 21 adalah menghasilkan generasi yang memiliki keterampilan berpikir kritis, memecahkan masalah, berkomunikasi, kreatif, inovatif dan mampu berkolaborasi. Untuk membentuk sumber daya manusia yang berkualitas tersebut, maka dibutuhkan tenaga pendidik yang siap mengajar dan mendidik melalui pembelajaran abad 21 yang relevan dengan perkembangan era revolusi industri 4.0. Keterampilan kolaboratif dan berpikir kritis dapat diukur melalui asesmen autentik yang mampu mempersiapkan mahasiswa dalam menghadapi era tersebut. Asesmen autentik adalah suatu bentuk penilaian yang meminta peserta didik untuk menunjukkan kinerja dalam konteks dunia nyata yang menunjukkan aplikasi bermakna dari penerapan pengetahuan, sikap, dan keterampilan. Penelitian ini bertujuan untuk menghasilkan asesmen autentik dengan menggunakan metode jigsaw untuk mengukur kemampuan kolaboratif dan berpikir kritis mahasiswa pendidikan biologi. Penelitian ini merupakan penelitian pengembangan (Research & Development). Model yang digunakan adalah model ADDIE yaitu analisis, desain, mengembangkan, implementasi dan evaluasi. Sampel penelitian ini mahasiswa semester VII yang berjumlah 20 orang yang diambil secara purposive sampling. Analisis data dilakukan dengan teknik deskriptif statistik. Hasil penelitian telah diperoleh produk asesmen autentik berupa lembar observasi kemampuan kolaborasi dan tes kemampuan berpikir kritris melalui metode jigsaw. Asesmen yang dihasilkan telah dinyatakan valid dan reliabel berdasarkan hasil validasi ahli dan validasi lapangan.
Etnobotani Tumbuhan Pewarna Alami Kain Tenun pada Suku Baduy Luar Provinsi Banten
Authors: Mukhoyyaroh Qiswatun , L. S. Nugraheni  , N. Utami  
Published: January 2025

This research aims to identify the types, habitus, parts/organs and processing methods of plants used as natural materials for woven cloth from the Outer Baduy Banten tribe. Apart from that, this research also aims to identify the colors produced from plants used as natural materials for the woven cloth of the Baduy Luar tribe. The research approach uses quantitative with exploratory survey methods. Determination of the sample using purposive sampling technique. The samples chosen were deliberately determined based on certain criteria, namely someone who understands the dyeing of woven fabric in the Baduy community and someone who understands weaving techniques in the Outer Baduy tribe. The number of samples from the Outer Baduy tribe was 5 people. The results of the research show that the natural dye plants found in the Outer Baduy tribe are 9 types of plants, consisting of 8 families, 3 habituses (trees, bushes and shrubs), 5 plant organs that are used (bark, leaves, rhizomes, stems and roots), The majority of processing methods involve boiling the parts of the organs used and then soaking them, the resulting colors are yellow, blue, brown, red and black.
Uji Potensi Bee Pollen Trigona incisa Menurunkan Kadar Gula Darah Mencit (Mus musculus) yang Dibebani Glukosa
Authors: Kurniati Reni , Fatmawati Patang  , Desy Rara Bulaan  , Nova Hariani  
Published: January 2025

Bee polen lebah kelulut (Trigona incisa ) secara empiris dipercaya dapat menurunkan gula darah. Namun sampai saat ini belum ada penelitian yang membuktikan bahwa bee pollen ini berkhasiat sebagai penurun kadar gula darah. Penelitian ini bertujuan untuk menguji potensi bee pollen Trigona incisa dalam menurunkan kadar gula darah mencit (Mus musculus) yang dibebani glukosa. Mencit 25 ekor akan dibagi 5 kelompok perlakuan. Perlakuan I adalah kelompok kontrol negatif diberi 1mL CMC 1%, Perlakuan II adalah kontrol posistif diberi glibenklamid 0,65 mg/bb dalam 1mL CMC 1%. Perlakuan III, IV dan V diberi bee pollen dengan dosis 0,125 mg, 0,25 mg dan 0,50 mg masing dalam 1mL CMC 1%. Mencit dipuasakan selama 16 jam sebelumnya dan diukur kadar gula darah puasanya. Menit ke-30 , semua mencit diberi glukosa 50% sebanyak 3 g/kgbb. Pengukuran kadar gula darah mencit selanjutnya setelah menit ke 30, 60,90,120, 150 dan 180 dengan alat glukometer. Data dianalisis dengan ANOVA dan uji lanjut LSD. Hasil penelitian ini menunjukkan bahwa pemberian bee polen Trigona incisa pada dosis 0,125 - 0,50 mg terbukti dapat menurunkan kadar gula darah sebanding bahkan lebih baik dibandingkan kontrol positif. Bee pollen dapat menurunkan kadar gula darah mencit 12,50- 24,30 %
Literatur Review : Analisis Kemampuan Literasi Visual Peserta Didik Pada Pembelajaran Biologi di Sekolah
Authors: Arminianti_wau  , Muhyiatul Fadilah  
Published: January 2025

Materi biologi termasuk materi yang sulit dipahami oleh peserta didik karena bersifat abstrak dan banyak memuat unsur visual sehingga peserta perlu memiliki kemampuan literasi visual untuk membantu memahami dan menginterprestasikan makna visual pada materi biologi. Artikel ini bertujuan untuk menyajikan tinjauan literatur mengenai analisis kemampuan literasi visual peserta didik pada pembelajaran biologi. Penelitian ini merupakan jenis penelitian tinjauan pustaka (literature review). Artikel yang dikaji sebanyak tigabelas yang diperoleh dari google scholar dengan kata kunci kemampuan literasi visual sesuai dengan tujuan penelitian yang diterbitkan pada tahun 2017-2024. Hasil literature review menunjukkan bahwa kemampuan literasi visual peserta didik berbeda-beda di setiap artikel mulai dari kategori rendah, sedang, cukup, maupun tinggi
Aktivitas Ekstrak Etanol Bunga Kertas (Bougainvillea spectabilis) terhadap Pertumbuhan Candida albicans
Authors: Apriliani Trisna , Pratiwi Rina Hidayati , Noer Shafa 
Published: January 2025

Tujuan dari penelitian ini adalah untuk mengetahui aktivitas ekstrak etanol bunga kertas (Bougainvillea spectabilis) terhadap pertumbuhan Candida albicans. Penelitian ini dilakukan di Laboratorium Universitas Indraprasta PGRI Jakarta. Metode penelitian yang digunakan adalah kuantitatif eksperimen dengan 5 kelompok perlakuan yaitu ekstrak bunga kertas (Bougainvillea spectabilis) dengan konsentrasi P1=5%, P2=10% dan P3=15% serta kontrol positif ketokonazol (0,1%) dan kontrol negatif aquades. Berdasarkan hasil uji aktivitas antijamur yang dilakukan dengan metode difusi kertas cakram didapatkan hasil ekstrak bunga kertas (Bougainvillea spectabilis) sebanyak 3 kali pengulangan dalam 3 batch yaitu zona hambat terbesar pada konsentrasi 15% sebesar 11,6 mm dan zona hambat terkecil pada konsentrasi 5% sebesar 6,50 mm. Dengan demikian, ekstrak etanol bunga kertas (Bougainvillea spectabilis) memiliki daya antijamur terhadap pertumbuhan Candida albicans.