bluuebunny/crossref_metadata_2025_split huggingface dataset:
Dynamic lane-changing game model in mixed autonomous and human-driven vehicle environment
Authors: Cao Yuqing , Sun Hao , Sun Panfei 
Published: July 2023

Abstract
Lane changing in mixed environments is a critical task that requires autonomous vehicles(AV) to respond to constantly changing traffic conditions in real time, while dealing with the uncertainties introduced by human-driven vehicles(HV). This paper proposes a dynamic lane-changing game model that captures the dynamic interaction among vehicles during lane-changing. To address the uncertainty in human drivers' intentions in the mixed environment, uncertain parameter is introduced that capture their diverse payoff preferences. When tackling the lack of information exchange between AV and HV, a strategy prediction algorithm based on the introduced parameter is proposed, enabling AV to predict the action of HV. The simulation is conducted using Next Generation Simulation (NGSIM) data to verify the effectiveness of the algorithm. The results indicate that the presence of algorithmic strategy assistance enables AV to consistently perform safe and efficient lane-changing maneuvers in the presence of parameter variations in human drivers.
Tendon stem/progenitor cells are promising reparative cell sources for multiple musculoskeletal injuries of concomitant articular cartilage lesions associated with ligament injuries
Authors: Yin Heyong , Mao Kelei , Huang Yufu , Ding Yufei , Dai Yike , Li Zhiyao , Guo Ai , Ma Lifeng , Diao Naicheng , Shi Lin 
Published: July 2023

Abstract
Background
Trauma-related articular cartilage lesions usually occur in conjunction with ligament injuries. Torn ligaments are frequently reconstructed with autologous tendon autograft and has been proven to achieve satisfactory clinical outcomes. However, effective treatments for the concomitant articular cartilage lesions are still very insufficient. The current study was aimed to evaluate whether stem/progenitor cells derived from tendon tissue can be considered as an alternative reparative cell source for cartilage repair.
Methods
Decellularization cartilage matrix (DCM) was used to provide a chondrogenic microenvironment for human tendon stem/progenitor cells (hTSPCs). Live/dead staining and proliferative assay demonstrated that DCM supported survival and proliferation of hTSPCs with an excellent biocompatibility. Furthermore, gene expression analysis indicated that chondrogenic differentiation of hTSPC was induced by the DCM microenvironment, without the use of exogenous growth factors.
Results
TSPCs are promising reparative cell sources for promoting cartilage repair. Especially, in the cohort that articular cartilage lesions occur in conjunction with ligament injuries, autologous TSPCs can be isolated from a portion of the tendon autograph harvested for ligaments reconstruction.
Conclusion
To our knowledge, this is the first article that propose to isolate TSPCs from tendon autograph for enhancing musculoskeletal repair of concomitant articular cartilage lesions associated with ligament injuries. In future clinical practice, combined ligament reconstruction with TSPCs- based therapy which isolated from tendon autograft can to be considered to achieve superior restore of these associated injuries in musculoskeletal system.
Distinct non-synonymous mutations in cytochrome b highly correlate with decoquinate resistance in apicomplexan parasite Eimeria tenella
Authors: Hao Zhenkai , Chen Junmin , Sun Pei , Chen Linlin , Zhang Yuanyuan , Chen Wenxuan , Hu Dandan , Bi Feifei , Han Zhenyan , Tang Xinming , Suo Jingxia , Suo Xun , Liu Xianyong 
Published: July 2023

Abstract
Background
Protozoan parasites of the genus Eimeria are the causative agents of chicken coccidiosis. Parasite resistance to most anticoccidial drugs is one of the major challenges in controlling this disease. There is an urgent need for a molecular marker to monitor the emergence of resistance against anticoccidial drugs, such as decoquinate.
Methods
In this study, we developed decoquinate-resistant strains by successively exposing the Houghton (H) and Xinjiang (XJ) strains of E. tenella to incremental concentrations of the drug in chickens. Additionally, we isolated a decoquinate-resistant strain from the field. The resistance of these three strains was tested using the criteria of weight gain (WG), relative oocyst production (ROP), and reduction of lesion scores (RLS). Whole-genome sequencing was used to identify the non-synonymous mutations in coding genes that were highly associated with the decoquinate-resistant phenotype in the two laboratory-induced strains. Subsequently, we further scrutinized the missense mutation in a field-resistant strain for verification. We employed AlphaFold and PyMOL to model the alterations in the binding affinity of the mutants towards the drug molecule.
Results
We obtained two decoquinate-resistant strains, DecR_H and XJ, originating from the H and XJ strains, respectively, as well as a field-resistant E. tenella strain, DecR_SC. These three strains displayed resistant to 120 mg/kg decoquinate administered through feed. Through whole-genome sequencing analysis, we identified the cytochrome b gene (ETH2_MIT00100) as the sole mutated gene shared between the DecR_H and XJ strains and was also detected in the DecR_SC strain. Distinct non-synonymous mutations, namely Gln131Lys in the DecR_H, Phe263Leu in the DecR_XJ, and Phe283Leu in the DecR_SC were observed in the three resistant strains. Notably, these mutations were located in the extracellular segment of cytochrome b, in close proximity to the ubiquinol oxidation site Qo. Drug molecular docking studies revealed that these mutants exhibited varying degrees of reduced binding ability to decoquinate.
Conclusions
Our findings emphasize the critical role of cytochrome b mutations in the development of decoquinate resistance in E. tenella. The strong correlation observed between cytochrome b mutant alleles and resistance indicates their potential as valuable molecular markers for the rapid detection of decoquinate resistance.
Dolutegravir use over 48 weeks is not associated with worsening insulin resistance and pancreatic beta cell function in a cohort of HIV-infected Ugandan adults
Authors: Mulindwa Frank , Castelnuovo Barbara , Brusselaers Nele , Nabwana Martin , Bollinger Robert , Laker Eva , Kiguba Ronald , Schwarz Jean-Marc 
Published: July 2023

Abstract
Background
The Uganda Ministry of Health issued restrictive guidelines on the use of dolutegravir (DTG) in persons stratified to have a heightened risk of diabetes mellitus. This followed multiple reports of persons with HIV (PWH) presenting with accelerated hyperglycemia after a few weeks to months of exposure to DTG. Having demonstrated a low incidence of diabetes mellitus and improving blood glucose trajectories in a cohort of ART naïve Ugandan PWH on DTG, we sought to determine whether the observed improvement in blood glucose did not mask background compensated insulin resistance.
Methods
In this analysis, 63 patients underwent serial oral glucose tolerance tests over 48 weeks. Using fasting serum insulin and glucose, we calculated insulin resistance and pancreatic beta cell function by homeostatic modelling (HOMA IR and HOMA%β respectively). Absolute mean changes between baseline and post-baseline blood glucose, pancreatic beta cell function and insulin resistance were computed by subtracting each post-baseline value from the baseline value and compared using student t-test. Multiple linear regression models were used to determine the factors associated with changes in pancreatic beta cell function and insulin resistance.
Results
Of the 63 participants, 37 (58%) were female. Median age was 31 (IQR: 28–37). Despite a trend towards an initial increase in both HOMA IR and HOMA%β at 12 weeks followed by a decline through 36 weeks to 48 weeks, the HOMA IR and HOMA%β at 48 weeks were not significantly different from baseline i.e. (difference in mean HOMA IR from baseline: 0.14, 95%CI: -0.46, 0.733, p = 0.648) and (difference in mean HOMA %β from baseline: 6.7, 95%CI: -13.4, 26.8, p = 0.506) respectively.
Wrinkles improvement in facial and periorbital regions with two different radio-frequencies from the same device head
Authors: Xu Yafei , Ai Ziying , Liu Huan , Tan Yimei 
Published: July 2023

Abstract
Objective: To evaluate efficacy of the new and unique radio- frequencies (RF) poles on facial and periorbital wrinkles.
Methods: Thirty-five healthy Chinese women with facial and periorbital wrinkles (age range 30-50 years) were enrolled in this study. Participants were treated with this device five times each week at home independently for 12 weeks. Four visits were scheduled during the trial (baseline, 28th day, 56th day and 84th day). 3D topographic photographs of periorbital wrinkles were obtained and then analyzed by Primos CR. The facial images were captured by Visia-CR, Image-Pro-Plus image software was used to analyze wrinkles in facial areas. The clinical grade evaluation on horizontal neck fold was conducted by dermatologists based on the "SKIN AGING ATLAS VOLUME 2-ASIAN TYPE".
Results: With the increase in treatment time, the wrinkles area and wrinkles area ratios of forehead wrinkles, nasolabial folds, and glabellar wrinkles decreased significantly. The data shows positive changes in crow's feet and underneath eye wrinkles after treatment, and the size of eye bags reduced. Besides, the skin color and firmness also significantly improved. Moreover, the scores in horizontal neck fold decreased by 10.14% on the 84th day compared to baseline.
Conclusion: This study shows that the home-use RF device equipped with a special electrode head can effectively reduce wrinkles in the eyes and face, as well as improve skin condition.
Prognosis and Immune Infiltration Analysis of m6A RNA methylation regulators in Skin cutaneous melanoma and differential analysis with cuproptosis
Authors: Tian Cui-cui , Shi Hao-ze , Zhang Cong-cong , Kong Ying-qi , Chen Hao 
Published: July 2023

Abstract
Background
Skin cutaneous melanoma (SKCM) is the most malignant tumor among skin cancers. We aimed to identify the role of m6A regulated genes in the prognosis and tumor immune microenvironment of SKCM. We further elevated the expression level of cuproptosis-related genes in different risk groups.
Methods
The transcriptome data of 471 SKCM tissues from the XENA database and 812 normal samples from GTEx were obtained.The univariate Cox regression analysis was used to explore the relationship of m6A related genes and the clinical outcomes of SKCM The least absolute shrinkage and selection operator (LASSO) regression analysis was used to construct a risk model for m6A regulated genes. Furthermore, Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis were performed. Also, the immune cell infiltration was analyzed using CIBERSORT. In addition, we assessed the expression level of cuproptosis-related genes in SKCM samples and its role in different risk sets. Expression validation of cuproptosis-associated genes in YTHDF3 downregulated melanoma cell lines by RT-qPCR.
Results
In this study, we found that m6A-related genes were differentially expressed in melanoma and normal samples. Eight of them were significantly related to prognosis. The risk model based on HNRNPA2B1, METTL3, WTAP, METTL15, YTHDC1 and RBMX was constructed and validated. Besides, the biological processes and signaling pathway were analyzed by GO and KEGG analysis. Moreover, the infiltration of immune cells in different risk sets were explored and we discovered the infiltration level of CD8+ T cells, T cell CD4+ memory activated, NK cell activated and Macrophage M1 was negatively correlated with risk score. We further detected the expression level of ten cuproptosis-related gens in different risk groups. Among them, the MTF1, PDHB and FDX1 were differentially expressed and the lower expression appeared to correlate with higher risk score. Finally, we found that the expression of MTF1 and PDHB was increased when m6A regulator YTHDF3 was downregulated.
Conclusion
Our study demonstrated the prognostic value of m6A-related genes in SKCM and their role in tumor immune microenvironment. In addition, we creatively explored the relationship between m6A RNA methylation regulators and cuproptosis-related genes in SKCM.
From the Perception to the Implementation of Culturally Responsive Teaching of an Elementary School Teacher
Authors: Hamidizadeh Katayoon 
Published: July 2023

Abstract
Teachers' perceptions of multicultural education have been investigated in many researches. This study has investigated the perception and implementation of culturally responsive teaching of an elementary school teacher in the classroom, that how she understands multicultural education and how she implements this understanding in her classroom. The analyses prove that the participating teacher had a limited understanding of culturally responsive teaching, and implemented it in a weak and limited way in her classroom.
Ferroptosis-induced Cardiotoxicity and Antitumor Drugs
Authors: Beretta Giovanni Luca 
Published: July 2023

:
The induction of regulated cell death ferroptosis in tumors is emerging as an
intriguing strategy for cancer treatment. Numerous antitumor drugs (e.g., doxorubicin,
etoposide, tyrosine kinase inhibitors, trastuzumab, arsenic trioxide, 5-fluorouracil) induce
ferroptosis. Although this mechanism of action is interesting for fighting tumors, the clinical
use of drugs that induce ferroptosis is hampered by cardiotoxicity. Besides in cancer
cells, ferroptosis induced by chemotherapeutics can occur in cardiomyocytes, and this
feature represents an important drawback of antitumor therapy. This inconvenience has
been tackled by developing less or no cardiotoxic antitumor drugs or by discovering cardioprotective
agents (e.g., berberine, propofol, fisetin, salidroside, melatonin, epigallocatechin-
3gallate, resveratrol) to use in combination with conventional chemotherapeutics.
This review briefly summarizes the molecular mechanisms of ferroptosis and describes
the ferroptosis dependent mechanisms responsible for cardiac toxicity developed by cancer-
suffering patients following the administration of some chemotherapeutics. Additionally,
the pharmacological strategies very recently proposed for potentially preventing this
inconvenience are considered.
The Combined Effects of Short-Term Exposure to Multiple Meteorological Factors on Unintentional Drowning Mortality: Large Case-Crossover Study
Authors: Liu Yingyin , Dong Xiaomei , Li Zhixing , Zhu Sui , Lin Ziqiang , He Guanhao , Gong Weiwei , Hu Jianxiong , Hou Zhulin , Meng Ruilin , Zhou Chunliang , Yu Min , Huang Biao , Lin Lifeng , Xiao Jianpeng , Zhong Jieming , Jin Donghui , Xu Yiqing , Lv Lingshuang , Huang Cunrui , Liu Tao , Ma Wenjun 
Published: July 2023

Background
Drowning is a serious public health problem worldwide. Previous epidemiological studies on the association between meteorological factors and drowning mainly focused on individual weather factors, and the combined effect of mixed exposure to multiple meteorological factors on drowning is unclear.

Objective
We aimed to investigate the combined effects of multiple meteorological factors on unintentional drowning mortality in China and to identify the important meteorological factors contributing to drowning mortality.

Methods
Unintentional drowning death data (based on International Classification of Diseases, 10th Edition, codes W65-74) from January 1, 2013, to December 31, 2018, were collected from the Disease Surveillance Points System for Guangdong, Hunan, Zhejiang, Yunnan, and Jilin Provinces, China. Daily meteorological data, including daily mean temperature, relative humidity, sunlight duration, and rainfall in the same period were obtained from the Chinese Academy of Meteorological Science Data Center. We constructed a time-stratified case-crossover design and applied a generalized additive model to examine the effect of individual weather factors on drowning mortality, and then used quantile g-computation to estimate the joint effect of the mixed exposure to meteorological factors.

Results
A total of 46,179 drowning deaths were reported in the 5 provinces in China from 2013 to 2018. In an effect analysis of individual exposure, we observed a positive effect for sunlight duration, a negative effect for relative humidity, and U-shaped associations for temperature and rainfall with drowning mortality. In a joint effect analysis of the above 4 meteorological factors, a 2.99% (95% CI 0.26%-5.80%) increase in drowning mortality was observed per quartile rise in exposure mixture. For the total population, sunlight duration was the most important weather factor for drowning mortality, with a 93.1% positive contribution to the overall effects, while rainfall was mainly a negative factor for drowning deaths (90.5%) and temperature and relative humidity contributed 6.9% and –9.5% to the overall effects, respectively.

Conclusions
This study found that mixed exposure to temperature, relative humidity, sunlight duration, and rainfall was positively associated with drowning mortality and that sunlight duration, rather than temperature, may be the most important meteorological factor for drowning mortality. These findings imply that it is necessary to incorporate sunshine hours and temperature into early warning systems for drowning prevention in the future.
Perancangan Security Information and Event Management (SIEM) untuk Mendeteksi Insiden pada Situs Web
Authors: Sijabat Daniel Rudiaman , Evo Stevanus 
Published: July 2023

Dalam era digital yang semakin kompleks, situs web menjadi sasaran utama serangan oleh pihak yang tidak bertanggung jawab. Oleh karena itu, diperlukan sistem yang dapat memantau, menganalisis, dan memberikan peringatan dini terhadap aktivitas mencurigakan atau serangan yang terjadi pada situs web. Untuk itu pada penelitian ini dilakukan analisis dan perancangan sebuah sistem Security Information and Event Management (SIEM) yang dapat digunakan untuk mendeteksi insiden serangan pada situs web. SIEM yang dibangun pada penelitian ini menggunakan Elastic Stack yang akan digunakan untuk menganalisa, mengawasi, mendeteksi, dan menyimpan informasi event-event atau log-log keamanan pada tiap-tiap agent yang terkoneksi. Untuk mensimulasikan pengujian jenis-jenis serangan pada situs web seperti pada pada OWASP TOP 10 2017, digunakan beberapa aplikasi web yang rentan, yaitu DVWA, XVWA, dan MUTILLIDAE. Pengujian pada sistem SIEM yang dibuat menunjukkan sistem mampu mendeteksi jenis-jenis serangan terhadap situs web seperti yang disebutkan pada pada OWASP TOP 10 2017.
Repetitive transcranial magnetic stimulation can improve the fixation of eyes rather than the fixation preference in children with autism spectrum disorder
Authors: Tian Li , Ma Shuai , Li Yin , Zhao Meng-fei , Xu Chang , Wang Chen , Zhang Xin , Gao Lei 
Published: July 2023

BackgroundTranscranial magnetic stimulation (TMS) has been introduced into the intervention of autism spectrum disorders (ASD) as a possible new therapeutic option for modifying pathological neuroplasticity. However, the stimulating protocols of rTMS for ASD have not been approved unanimously, which affects the clinical popularization and application of rTMS. In addition, there is little research on the improvement of social processing of autistic children by rTMS.MethodsWe explored the clinical efficacy of rTMS and improvement of face processing with the protocol of left high-frequency and right low-frequency on bilateral dorsolateral prefrontal cortex (DLPFC), with a sample of 45 ASD participants aged 2–18.ResultsOur results showed that both the score on the Childhood Autism Rating Scale (CARS) and the fixations on the eyes of the human faces improved by two-session rTMS intervention, except for the percentage of eyes fixation. The mediation analysis indicated the item of “Adaptation to Change” of CARS mediated dominantly the improvement of eye-gaze behavior of ASD participants by rTMS.ConclusionOur study revealed the mechanism of rTMS in improving the eye-gaze behavior of the autism population, deepened the understanding of the function of rTMS in treating autistic social disorders, and provided a reference for combined treatment for ASD.
Brain structure alterations in girls with central precocious puberty
Authors: Yoshii Shoko , Takatani Tomozumi , Shiohama Tadashi , Takatani Rieko , Konda Yutaka , Hattori Shinya , Yokota Hajime , Hamada Hiromichi 
Published: July 2023

PurposeCentral precocious puberty (CPP) is puberty that occurs at an unusually early age with several negative psychological outcomes. There is a paucity of data on the morphological characteristics of the brain in CPP. This study aimed to determine the structural differences in the brain of patients with CPP.MethodsWe performed voxel- and surface-based morphometric analyses of 1.5 T T1-weighted brain images scanned from 15 girls with CPP and 13 age-matched non-CPP controls (NC). All patients with CPP were diagnosed by gonadotropin-releasing hormone (GnRH) stimulation test. The magnetic resonance imaging (MRI) data were evaluated using Levene’s test for equality of variances and a two-tailed unpaired t-test for equality of means. False discovery rate correction for multiple comparisons was applied using the Benjamini–Hochberg procedure.ResultsMorphometric analyses of the brain scans identified 33 candidate measurements. Subsequently, increased thickness of the right precuneus was identified in the patients with CPP using general linear models and visualizations of cortical thickness with a t-statistical map and a random field theory map.ConclusionThe brain scans of the patients with CPP showed specific morphological differences to those of the control. The features of brain morphology in CPP identified in this study could contribute to further understanding the association between CPP and detrimental psychological outcomes.