bluuebunny/crossref_metadata_2025_split huggingface dataset:
Loss of pex5 sensitizes zebrafish to fasting due to deregulated mitochondria, mTOR, and autophagy
Authors: Bhandari Sushil , Kim Yong-Il , Nam In-Koo , Hong KwangHeum , Jo Yunju , Yoo Kyeong-Won , Liao Weifang , Lim Jae-Young , Kim Seong-Jin , Um Jae-Young , Kim Peter K. , Lee Ho Sub , Ryu Dongryeol , Kim Seok-Hyung , Kwak SeongAe , Park Raekil , Choe Seong-Kyu 
Published: February 2023

AbstractAnimal models have been utilized to understand the pathogenesis of Zellweger spectrum disorders (ZSDs); however, the link between clinical manifestations and molecular pathways has not yet been clearly established. We generated peroxin 5 homozygous mutant zebrafish (pex5−/−) to gain insight into the molecular pathogenesis of peroxisome dysfunction. pex5−/− display hallmarks of ZSD in humans and die within one month after birth. Fasting rapidly depletes lipids and glycogen in pex5−/− livers and expedites their mortality. Mechanistically, deregulated mitochondria and mechanistic target of rapamycin (mTOR) signaling act together to induce metabolic alterations that deplete hepatic nutrients and accumulate damaged mitochondria. Accordingly, chemical interventions blocking either the mitochondrial function or mTOR complex 1 (mTORC1) or a combination of both improve the metabolic imbalance shown in the fasted pex5−/− livers and extend the survival of animals. In addition, the suppression of oxidative stress by N-acetyl L-cysteine (NAC) treatment rescued the apoptotic cell death and early mortality observed in pex5−/−. Furthermore, an autophagy activator effectively ameliorated the early mortality of fasted pex5−/−. These results suggest that fasting may be detrimental to patients with peroxisome dysfunction, and that modulating the mitochondria, mTORC1, autophagy activities, or oxidative stress may provide a therapeutic option to alleviate the symptoms of peroxisomal diseases associated with metabolic dysfunction.
Mfsd2a attenuated hypoxic-ischemic brain damage via protection of the blood–brain barrier in mfat-1 transgenic mice
Authors: Li Xiaoxue , Zhang Yumeng , Chang Jianghao , Zhang Chenglin , Li Lin , Dai Yifan , Yang Haiyuan , Wang Ying 
Published: February 2023

AbstractPrevious studies have shown that mfat-1 transgenic mice have protective effects against some central nervous system (CNS) disorders, owing to the high docosahexaenoic acid (DHA) content enriched in their brains. However, whether this protective effect is connected to the blood–brain barrier (BBB) remains unclear. This study aims to investigate the mechanisms of the protective effect against hypoxic-ischemic brain damage (HIBD) of mfat-1 transgenic mice. mfat-1 mice not only demonstrated a significant amelioration of neurological dysfunction and neuronal damage but also partly maintained the physiological permeability of the BBB after HIBD. We initially showed this was associated with elevated major facilitator superfamily domain-containing 2a (Mfsd2a) expression on the BBB, resulting from more lysophosphatidylcholine (LPC)-DHA entering the brain. Wild-type (WT) mice showed a similar Mfsd2a expression trend after long-term feeding with an LPC-DHA-rich diet. Knockdown of Mfsd2a by siRNA intra-cerebroventricular (ICV) injection neutralized the protective effect against HIBD-induced BBB disruption in mfat-1 mice, further validating the protective function of Mfsd2a on BBB. HIBD-induced BBB high permeability was attenuated by Mfsd2a, primarily through a transcellular pathway to decrease caveolae-like vesicle-mediated transcytosis. Taken together, these findings not only reveal that mfat-1 transgenic mice have higher expression of Mfsd2a on the BBB, which partly sustains BBB permeability via vesicular transcytosis to alleviate the severity of HIBD, but also suggest that dietary intake of LPC-DHA may upregulate Mfsd2a expression as a novel therapeutic strategy for BBB dysfunction and survival in HIBD patients.
Analytical Properties for a Stochastic Rotating Shallow Water Model Under Location Uncertainty
Authors: Lang Oana , Crisan Dan , Mémin Étienne 
Published: February 2023

AbstractThe rotating shallow water model is a simplification of oceanic and atmospheric general circulation models that are used in many applications such as surge prediction, tsunami tracking and ocean modelling. In this paper we introduce a class of rotating shallow water models which are stochastically perturbed in order to incorporate model uncertainty into the underlying system. The stochasticity is chosen in a judicious way, by following the principles of location uncertainty, as introduced in Mémin (Geophys Astrophys Fluid Dyn 108(2):119–146, 2014). We prove that the resulting equation is part of a class of stochastic partial differential equations that have unique maximal strong solutions. The methodology is based on the construction of an approximating sequence of models taking value in an appropriately chosen finite-dimensional Littlewood-Paley space. Finally, we show that a distinguished element of this class of stochastic partial differential equations has a global weak solution.
Sex differences in body composition in people with prediabetes and type 2 diabetes as compared with people with normal glucose metabolism: the Maastricht Study
Authors: de Ritter Rianneke , Sep Simone J. S. , van Greevenbroek Marleen M. J. , Kusters Yvo H. A. M. , Vos Rimke C. , Bots Michiel L. , Kooi M. Eline , Dagnelie Pieter C. , Eussen Simone J. P. M. , Schram Miranda T. , Koster Annemarie , Brouwers Martijn C. G. , van der Sangen Niels M. R. , Peters Sanne A. E. , van der Kallen Carla J. H. , Stehouwer Coen D. A. 
Published: February 2023

Abstract
Aims/hypothesis
Obesity is a major risk factor for type 2 diabetes. However, body composition differs between women and men. In this study we investigate the association between diabetes status and body composition and whether this association is moderated by sex.

Methods
In a population-based cohort study (n=7639; age 40–75 years, 50% women, 25% type 2 diabetes), we estimated the sex-specific associations, and differences therein, of prediabetes (i.e. impaired fasting glucose and/or impaired glucose tolerance) and type 2 diabetes (reference: normal glucose metabolism [NGM]) with dual-energy x-ray absorptiometry (DEXA)- and MRI-derived measures of body composition and with hip circumference. Sex differences were analysed using adjusted regression models with interaction terms of sex-by-diabetes status.

Results
Compared with their NGM counterparts, both women and men with prediabetes and type 2 diabetes had more fat and lean mass and a greater hip circumference. The differences in subcutaneous adipose tissue, hip circumference and total and peripheral lean mass between type 2 diabetes and NGM were greater in women than men (women minus men [W–M] mean difference [95% CI]: 15.0 cm2 [1.5, 28.5], 3.2 cm [2.2, 4.1], 690 g [8, 1372] and 443 g [142, 744], respectively). The difference in visceral adipose tissue between type 2 diabetes and NGM was greater in men than women (W–M mean difference [95% CI]: −14.8 cm2 [−26.4, −3.1]). There was no sex difference in the percentage of liver fat between type 2 diabetes and NGM. The differences in measures of body composition between prediabetes and NGM were generally in the same direction, but were not significantly different between women and men.

Conclusions/interpretation
This study indicates that there are sex differences in body composition associated with type 2 diabetes. The pathophysiological significance of these sex-associated differences requires further study.

Graphical abstract
Son preference and education Inequalities in India: the role of gender-biased fertility strategies and preferential treatment of boys
Authors: Congdon Fors Heather , Lindskog Annika 
Published: February 2023

Abstract
We investigate the impact of son preference in India on gender inequalities in education. We distinguish the impact of preferential treatment of boys from the impact of gender-biased fertility strategies (gender-specific fertility stopping rules and sex-selective abortions). Results show strong impacts of gender-biased fertility strategies on education inequalities between girls and boys. Preferential treatment of boys also matters but appears to have a more limited impact for most outcomes. Further, our results suggest that gender-biased fertility strategies create gender inequalities in education both because girls and boys end up in systematically different families and because of gender inequalities in pecuniary investment within families. Since gender inequalities in education in India are partially the result of gender-biased fertility strategies, they are not likely to disappear until the strong desire to have a son does so.
Cell-based therapies have disease-modifying effects on osteoarthritis in animal models. A systematic review by the ESSKA Orthobiologic Initiative. Part 2: bone marrow-derived cell-based injectable therapies
Authors: Boffa Angelo , Perucca Orfei Carlotta , Sourugeon Yosef , Laver Lior , Magalon Jérémy , Sánchez Mikel , Tischer Thomas , de Girolamo Laura , Filardo Giuseppe 
Published: February 2023

Abstract
Purpose
Aim of this systematic review was to determine if bone marrow-derived cell-based injectable therapies induce disease-modifying effects in joints affected by osteoarthritis (OA) in animal models.

Methods
A systematic review was performed on three electronic databases (PubMed, Web of Science, Embase) according to PRISMA guidelines. A synthesis of the results was performed investigating disease-modifying effects in preclinical animal studies comparing injectable bone marrow-derived products with OA controls or other products, different formulations or injection intervals, and the combination with other products. The risk of bias was assessed according to the SYRCLE’s tool.

Results
Fifty-three studies were included (1819 animals) with an increasing publication trend over time. Expanded cells were used in 48 studies, point-of-care products in 3 studies, and both approaches were investigated in 2 studies. Among the 47 studies presenting results on the disease-modifying effects, 40 studies (85%) reported better results with bone marrow-derived products compared to OA controls, with positive findings evident in 14 out of 20 studies (70%) in macroscopic assessment, in 30 out of 41 studies (73%) in histological assessment, and in 10 out of 13 studies (77%) in immunohistochemical evaluations. Clinical evaluations showed positive results in 7 studies out of 9 (78%), positive imaging results in 11 studies out of 17 (65%), and positive biomarker results in 5 studies out of 10 (50%). While 36 out of 46 studies (78%) reported positive results at the cartilage level, only 3 out of 10 studies (30%) could detect positive changes at the synovial level. The risk of bias was low in 42% of items, unclear in 50%, and high in 8%.

Conclusion
This systematic review of preclinical studies demonstrated that intra-articular injections of bone marrow-derived products can induce disease-modifying effects in the treatment of OA, slowing down the progression of cartilage damage with benefits at macroscopic, histological, and immunohistochemical levels. Positive results have been also observed in terms of clinical and imaging findings, as well as in the modulation of inflammatory and cartilage biomarkers, while poor effects have been described on the synovial membrane. These findings are important to understand the potential of bone marrow-derived products and to guide further research to optimise their use in the clinical practice.

Level of evidence
II.
Hodge sheaves underlying flat projective families
Authors: Kovács Sándor J. , Taji Behrouz 
Published: February 2023

AbstractWe show that, for any fixed weight, there is a natural system of Hodge sheaves, whose Higgs field has no poles, arising from a flat projective family of varieties parametrized by a regular complex base scheme, extending the analogous classical result for smooth projective families due to Griffiths. As an application, based on positivity of direct image sheaves, we establish a criterion for base spaces of rational Gorenstein families to be of general type. A key component of our arguments is centered around the construction of derived categorical objects generalizing relative logarithmic forms for smooth maps and their functorial properties.
Rapid determination of malondialdehyde in serum samples using a porphyrin-functionalized magnetic graphene oxide electrochemical sensor
Authors: Ben Attig Jihène , Latrous Latifa , Galvan Ismael , Zougagh Mohammed , Ríos Ángel 
Published: February 2023

Abstract
An electrochemical sensor based on a screen-printed carbon electrode (SPCE) modified with porphyrin-functionalized magnetic graphene oxide (TCPP-MGO) was developed for the sensitive and selective determination of malondialdehyde (MDA), an important biomarker of oxidative damage, in serum samples. The coupling of TCPP with MGO allows the exploitation of the magnetic properties of the material for separation, preconcentration, and manipulation of analyte, which is selectively captured onto the TCPP-MGO surface. The electron-transfer capability in the SPCE was improved through derivatization of MDA with diaminonaphthalene (DAN) (MDA-DAN). TCPP-MGO-SPCEs have been employed to monitor the differential pulse voltammetry (DVP) levels of the whole material, which is related to the amount of the captured analyte. Under optimum conditions, the nanocomposite-based sensing system has proved to be suitable for the monitoring of MDA, presenting a wide linear range (0.01–100 µM) with a correlation coefficient of 0.9996. The practical limit of quantification (P-LOQ) of the analyte was 0.010 µM, and the relative standard deviation (RSD) was 6.87% for 30 µM MDA concentration. Finally, the developed electrochemical sensor has demonstrated to be adequate for bioanalytical applications, presenting an excellent analytical performance for the routine monitoring of MDA in serum samples.
Graphical abstract
One-dimensional and comprehensive two-dimensional gas chromatographic approaches for the characterization of post-consumer recycled plastic materials
Authors: Hochegger Andrea , Pantò Sebastiano , Jones Nick , Leitner Erich 
Published: February 2023

AbstractIn September 2022, the European Commission published its new regulation on recycled plastic materials for food contact. It allows newly developed, non-authorized technologies and approaches, or so-called novel technologies, to be deployed in the field to generate the data needed for establishing regulatory and/or fit for purpose processes. The data shall be generated by using suitable methods, but the regulation does not give a more detailed description on those. In this study, commercially purchased buckets made of post-consumer recycled polypropylene were screened, using a number of different analytical approaches. Sample preparation methods, analysis techniques, and the data and information generated were compared. The results clearly demonstrate the need for a detailed characterization of such materials and the advantages and disadvantages of the analysis using conventional gas chromatography with flame ionization detection and mass spectrometery as well as two-dimensional comprehensive gas chromatography with time of flight mass spectrometry.
Graphical Abstract
Correlation Between Microbial Community and Hatching Failure in Loggerhead Sea Turtle Caretta caretta
Authors: Capri Fanny Claire , Prazzi Elena , Casamento Giulia , Gambino Delia , Cassata Giovanni , Alduina Rosa 
Published: February 2023

AbstractMicrobial communities provide essential information about host ecology and could be helpful as a tool to improve species conservation efforts. However, microbes can also infect and compromise the host development process and viability.Caretta carettais the most widespread marine turtle species in the Mediterranean basin and is the only species of sea turtle nesting along the Italian coasts. Little is known about the microbiota composition of the nest of sea turtles and its correlation with hatching failures. In this study, the microbial composition of two nests ofC. carettafeaturing different rates of hatching success from a nesting beach in Lampedusa (Italy) was analyzed and compared. The bacterial community was determined using culture-dependent methods and next-generation sequencing based on 16S rRNA gene metabarcoding analysis. Our results showed five dominant bacterial phyla (Proteobacteria, Bacteroidetes, Actinobacteria, Verrucomicrobia, and Firmicutes) and indicated different bacterial families (PseudomonadaceaeandBrucellaceae) as likely causes of hatching failures. Besides, our findings demonstrated the nests’ active role in modulating the sand’s bacterial communities. This study suggests microbiological analysis could be a valuable tool in monitoring nests to take preventive actions and reduce hatching failures.
Sex differences in dementia with Lewy bodies: an imaging study of neurotransmission pathways
Authors: Boccalini Cecilia , Nicastro Nicolas , Peretti Debora Elisa , Caminiti Silvia Paola , Perani Daniela , Garibotto Valentina 
Published: February 2023

Abstract
Purpose
Dementia with Lewy bodies (DLB) is characterized by a wide clinical and biological heterogeneity, with sex differences reported in both clinical and pathologically confirmed DLB cohorts. No research evidence is available on sex differences regarding molecular neurotransmission. This study aimed to assess whether sex can influence neurotransmitter systems in patients with probable DLB (pDLB).

Methods
We included 123 pDLB patients (male/female: 77/46) and 78 control subjects (male/female: 34/44) for comparison, who underwent 123I-FP-CIT SPECT imaging. We assessed sex differences in the dopaminergic activity of the nigrostriatal and mesolimbic systems using regional-based and voxel-wise analyses of 123I-FP-CIT binding. We tested whether sex-specific binding alterations would also pertain to the serotoninergic and noradrenergic systems by applying spatial correlation analyses. We applied molecular connectivity analyses to assess potential sex differences in the dopaminergic pathways.

Results
We found comparable 123I-FP-CIT binding decreases in the striatum for pDLB males and females compared to controls. However, pDLB females showed lower binding in the extrastriatal projections of the nigrostriatal and mesolimbic dopaminergic systems compared to pDLB males. According to the spatial correlation analysis, sex-specific molecular alterations were also associated with serotonergic and noradrenergic systems. Nigrostriatal and mesolimbic systems’ connectivity was impaired in both groups, with males showing local alterations and females presenting long-distance disconnections between subcortical and cortical regions.

Conclusions
Sex-specific differences in 123I-FP-CIT binding were found in our cohort, namely, a trend for lower 123I-FP-CIT binding in females, significant in the presence of a pDLB diagnosis. pDLB females showed also different patterns of connectivity compared to males, mostly involving extrastriatal regions. The results suggest the presence of a sex-related regional vulnerability to alpha-synuclein pathology, possibly complicated also by the higher prevalence of Alzheimer’s disease co-pathology in females, as previously reported in pDLB populations.
[89Zr]-immuno-PET prediction of response to rituximab treatment in patients with therapy refractory interstitial pneumonitis: a phase 2 trial
Authors: Adams H. , van de Garde E. M. W. , Vugts D. J. , Grutters J. C. , Oyen Wim. J.G. , Keijsers R. G. 
Published: February 2023

Abstract

Introduction
Immune-mediated interstitial pneumonitis may be treated with anti-CD20 therapy after failure of conventional therapies. However, clinical response is variable. It was hypothesized that autoreactive CD20-positive cells may play an important role in this variability. This prospective study aims to elucidate if imaging of CD20-positive cells in the lungs allows prediction of the response to anti-CD20 treatment.

Methods
Twenty-one patients with immune-mediated interstitial lung disease (ILD) with deteriorated pulmonary function received a dose of 1000 mg rituximab on day 1 and day 14 spiked with a tracer dose of radiolabeled [89Zr]-rituximab. PET/CT was performed on days 3 and 6. Standardized uptake values (SUV) were calculated as a measure for pulmonary CD20 expression. Based on pulmonary function tests (PFT), forced vital capacity (FVC), and diffusing capacity for carbon monoxide (DLCO), prior to and 6 months after treatment, patients were classified as responder (stable disease or improvement) or non-responder.

Results
Fifteen patients (71%) were classified as responder. Pulmonary [89Zr]-rituximab PET SUVmean was significantly correlated with the change in FVC and DLCO (K = 0.49 and 0.56, respectively) when using target-to-background ratios, but not when using SUVmean alone. [89Zr]-rituximab SUVmean was significantly higher in responders than in non-responders (0.35 SD 0.09 vs. 0.23 SD 0.06; P = 0.02).

Conclusion
Rituximab treatment was effective in the majority of patients. As a higher pulmonary uptake of [89Zr]-rituximab correlated with improvement of PFT and treatment outcome, [89Zr]-rituximab PET imaging may serve as a potential predictive biomarker for anti-CD20 therapy.

Trial registration
Clinicaltrials.gov identifier NCT02251964
Outcomes of Self-Expanding Covered Stents for the Treatment of External ILIAC Artery Obstructive Disease
Authors: Squizzato Francesco , Mosquera-Rey Vicente , Zanabili Al-Sibbai Amer , Camblor Santervas Lino Antonio , Pasqui Edoardo , Palasciano Giancarlo , de Donato Gianmarco , Alonso Pérez Manuel , Antonello Michele , Piazza Michele 
Published: February 2023

Abstract
Purpose
To describe the early results and mid-term patency rates of external iliac artery (EIA) stenting using self-expanding covered stents.

Methods
We conducted a multicenter retrospective study (2015–2021), including patients receiving primary endovascular treatment of external iliac artery occlusive disease with self-expanding covered stents. All patients were treated with the Viabahn (W.L Gore & Associates, Inc., Flagstaff, AZ–USA) stent. Patency and limb salvage rates were estimated with Kaplan–Meier curves.

Results
Ninety-three patients (mean age, 69 ± 9 years; 81% males) were treated for disabling claudication in 44%, rest pain in 28%, and tissue loss in 28%. TASC C/D lesions were present in 72% and iliac complete occlusion in 30%. Mean lesion length was 6.9 ± 2.4 cm; 30% had moderate/severe EIA calcifications; and the mean iliac tortuosity index was 1.17 ± 0.13. Technical success was 100%. There was one perioperative death (1.4%) and procedural complication rate was 6.5%. At 42 months (mean, 25 months), primary patency was 89.8% (95%CI 83–98); the presence of EIA tortuosity (tortuosity index > 1.25, 87.7 ± 11% vs 89.9 ± 8%; P = .6) or severe calcifications (87.6 ± 9% vs 96.0 ± 8%; P = .400) had no significant impact. After univariate analysis, the use of a stent with diameter < 8 mm (HR 8.5, 95%CI 3.24–14.22; P < .001) was negatively associated with primary patency.

Conclusions
The use of self-expanding covered stents provided excellent early and mid-term results in the treatment of obstructive disease of the EIA, also in cases of high EIA tortuosity and high grade of calcifications. The use of a < 8 mm-diameter stent was associated with a reduced primary patency.