bluuebunny/crossref_metadata_2025_split huggingface dataset:
ADAR1 is a prognostic biomarker and is correlated with immune infiltration in lung adenocarcinoma
Authors: Yang Wendi , Chen Kehong , Yu Qian , Liao Rongxin , He Hengqiu , Peng Yuan , Yang Zhenzhou , Zhang Xiaoyue 
Published: May 2023

AbstractBackgroundLung adenocarcinoma (LUAD) is a common subtype of non‐small cell lung cancer with high morbidity and mortality rates and is usually detected at advanced stages because of the early onset of metastasis. Adenosine deaminase RNA‐specific 1 (ADAR1) is an RNA editing enzyme that catalyzes the important physiological process of adenosine‐to‐inosine editing and has been shown to participate in the progression of LUAD. Increasing evidence has suggested that immune infiltration of the tumor immune microenvironment has prognostic value for most human solid organ malignancies; however, much is unknown about the functions of ADAR1.MethodsThe expression of ADAR1 was analyzed in The Cancer Genome Atlas ‐LUAD database and validated in our LUAD cohort. To assess the prognostic value of ADAR1, Kaplan‐Meier survival analyses and Cox regression analyses were carried out in LUAD cohorts. The association between ADAR1 and LUAD immune infiltrates via analyses of cell‐type identification by estimating relative subsets of known RNA transcripts. Furthermore, multiplex immunohistochemistry was used to confirm the relationship between ADAR1 expression and immune cells in the present cohort of patients with LUAD.ResultsADAR1 was highly expressed in LUAD tissues and closely correlated with lymph node metastasis (LNM) (p < 0.01), advanced tumor stage (p < 0.05), and poor patient prognosis (p < 0.01), thus indicating that increased ADAR1 contributed to the progression of LUAD. LUAD with high ADAR1 expression can metastasize to lymph nodes that express more ADAR1 than the primary lesion. In addition, M0 macrophages and M2 macrophages increased and CD4+T cells decreased in LUAD tissues with high ADAR1 expression. And the expression of ADAR1 in lymph node metastases was negatively correlated with the contents of CD4+T cells (p = 0.0017) and M1 macrophages (p = 0.0037).ConclusionThe findings of our study suggested that ADAR1 may be useful in predicting prognosis and LNM in LUAD, and may serve as a promising immune‐related molecular target for LUAD patients.
Impact of emotional appeal on non‐profit advertising: A neurophysiological analysis
Authors: Sandoval Pamela Simón , García‐Madariaga Jesús 
Published: May 2023

AbstractThe number of Non‐Profit Organisations (NPOs) has increased in the past years, and they rely on advertising to communicate their causes. Although the effectiveness of NPO advertising has been studied previously, there is still no consensus about which appeal (emotionally positive or negative) increases its effectiveness. On the other hand, the most prevalent research approaches to NPO advertising are traditional research methodologies based on declarative techniques. So, the purpose of this study is to identify which appeal (positive or negative) in NPO advertising is more effective at the three levels of effectiveness (perceptual or communicational effectiveness, psychological or attitudinal effectiveness and behavioural effectiveness), providing a new approach based on the analysis of consumers' unconscious responses to advertising. We conducted an experiment with 113 participants whose neurophysiological responses were evaluated through electroencephalogram (EEG) and eye tracking (ET) while they watched NPO advertisements with a randomly assigned emotional appeal (positive or negative). In addition, a survey revealed behavioural responses. The results showed that considering the psychological or attitudinal effectiveness, positively framed ads are more effective, as there is a positive effect on attitudes towards the ad and a higher positive emotional valence. On the other hand, in perceptual effectiveness, where attention is considered an important variable, the negatively framed ads showed more significant time in the area of interest (AOI) of the image area of the ad, and longer time in the AOI text was observed for positively framed ads. Furthermore, regarding behavioural effectiveness, negatively framed ads seem more effective in eliciting actual donations. The results suggest that a positive appeal is more effective in generating a more positive attitude and a positive emotional valence towards the advertisement, which could benefit the NPO in the long term. But a negative appeal is more effective if the goal is to achieve immediate donations.
Investigations of Antibacterial, Antioxidant, and Antidiabetic Potential of Extract and Its Active Fractions from the Leaves of Horsfieldia spicata (Roxb.) J. Sinclair
Authors: Megawati  , Ariani Novita , Minarti  , Darmawan Akhmad , Eka Prastya Muhammad 
Published: May 2023

AbstractThis study was undertaken to analyse the potential bioactivities including antibacterial, antioxidant and antidiabetic derived from the methanolic extract and the column chromatography ethyl acetate fraction (AcOEt Fr) of Horsfieldia spicata leaves. Methanolic extract and 4 other fractions was calculated for total phenol and flavonoid contents along with tested for antibacterial, antioxidant and antidiabetic properties. Interestingly, the AcOEt Fr had the highest value for total flavonoid content and the best antioxidant, and antidiabetic activities. Therefore, the AcOEt Fr was further separated using column chromatography technique for obtaining 9 selected fractions namely fraction 1 (F1) ‐ fraction 9 (F9) which were further tested. The results showed that the AcOEt column chromatography fractions namely F2, F3, F4 and F6 had the best clear inhibition antibacterial value against all bacterial tested. In addition, these fractions also exhibited better Minimum Inhibitory Concentrations (MIC) and Minimum Bactericidal Concentrations (MBC) values than others. Antioxidant, 2,2‐diphenylpicrylhydrazyl (DPPH) assayed indicated that AcOEt Fr had the strongest IC50 value of 47.30 μg/mL. Further, F4 column chromatography fraction showed the best inhibition against α‐Glucosidase enzyme related to antidiabetic activity with an IC50 value of 6.11 μg/mL. Liquid chromatography tandem‐mass spectrometry (LC/MS/MS) analysis identified that F4 derived from AcOEt fraction had several compounds belonging to the flavonoid and phenolics such as 3′,5‐dihydroxy‐7,4′‐dimethoxyflavone, 5,7‐dihydroxy‐3‐(4′‐hydroxybenzyl)chromone, and Kadsurenin I.
Natural Geranylated Sulfur‐Containing Amides with Inhibitory Effect on Th17 Differentiation
Authors: Ji Kai‐Long , Tang Yan‐Qing , Guo Zheng‐Yang , GongPan Pian‐Chou , Sun Peng , Lu Jian‐Mei , Dai Meng‐Yuan , Li Ting 
Published: May 2023

AbstractChemical investigation of medicinal plant Glycosmis lucida Wall. ex C. C. Huang leaves led to the production of ten compounds (1–10), including two previously unreported geranylated sulfur‐containing amides (1 and 2) and eight known ones (3–10). Structural characterization was carried out using comprehensive spectroscopic methods including NMR, MS and CD. The inhibitory effects of all isolates on Th17 differentiation were evaluated, of which compounds 1 and 6 significantly inhibited Th17 differentiation with IC50 values of 0.36 and 1.30 μM, respectively, while both 1 and 6 failed to bind to retinoic acid‐related orphan receptor gamma t (RORγt), suggesting that their inhibition of Th17 differentiation is independent of RORγt.
Photo‐Alternating Current‐Electro‐Fenton Process for Pollutant Removal and Energy Usage from Industrial Wastewater: Response Surface Approach Optimization of Operational Parameters
Authors: Perumal Asaithambi , Yesuf Mamuye Busier , Govindarajan Rajendran , Niju Subramaniapillai , Arun C. , Periyasamy Selvakumar , Pandiyarajan T. , Alemayehu Esayas 
Published: May 2023

AbstractDuring the last two decades, there has been an increase in interest the use of electrochemical and advanced oxidation technologies for treatment of industrial wastewater. Efficacy of several techniques for treating distilling industry wastewater (DIW), UV light, H2O2, combination of UV and H2O2, direct current (DC) and alternating current (AC)‐electro‐Fenton (EF), and UV combined with DC/AC+EF (UV+DC/AC+EF) processes were all examined. The findings revealed that compared to the UV, H2O2, UV/H2O2, DC/AC+EF, and UV+DC+EF technologies, the UV+AC+EF process resulted total color of 100 % and COD of 100 % elimination efficiency with low energy use 4 kWhr m−3. A response surface methodology (RSM) approach that relies on central composite design (CCD) was used to optimize the UV+AC+EF technology‘s parameters for treating DIW. Optimal conditions were obtained by utilizing mathematical and statistical methods in order to maximize COD elimination (89.50 %) and minimize energy consumption (2.775 kWhr m−3). The parameters for this experiment were as follows: UV power: 32 W, H2O2: 320 mg L−1, COD: 2400 mg L−1, CD: 0.2 A dm−2, and duration: 111 min. The combined efficiency of removing % COD using the UV, AC+EF, and UV+AC+EF processes was used to determine the synergistic effect, which was 13.65 %. As a consequence of this, treating industrial wastewater with a hybrid UV+AC+EF technique is significantly more successful.
Overview of emerging catalytic materials for electrochemical green ammonia synthesis and process
Authors: Chebrolu Venkata Thulasivarma , Jang Daehee , Rani Gokana Mohana , Lim Chaeeun , Yong Kijung , Kim Won Bae 
Published: May 2023

AbstractThe concept of “green‐ammonia‐zero‐carbon emission” is an emerging research topic in the global community and many countries driving toward decarbonizing a diversity of applications dependent on fossil fuels. In light of this, electrochemical nitrogen reduction reaction (ENRR) received great attention at ambient conditions. The low efficiency (%) and ammonia (NH3) production rates are two major challenges in making a sustainable future. Besides, hydrogen evolution reaction is another crucial factor for realizing this NH3 synthesis to meet the large‐scale commercial demand. Herein, the (i) importance of NH3 as an energy carrier for the next future, (ii) discussion with ENRR theory and the fundamental mechanism, (iii) device configuration and types of electrolytic systems for NH3 synthesis including key metrics, (iv) then moving into rising electrocatalysts for ENRR such as single‐atom catalysts (SACs), MXenes, and metal–organic frameworks that were scientifically summarized, and (v) finally, the current technical contests and future perceptions are discussed. Hence, this review aims to give insightful direction and a fresh motivation toward ENRR and the development of advanced electrocatalysts in terms of cost, efficiency, and technologically large scale for the synthesis of green NH3.
Synthesis and Characterization of Hexafluorocyclopentane‐Bridged Bisbutatrienes as Models for Longer Cumulenes: Various Transformations for the Construction of π‐Conjugated Frameworks
Authors: Konishi Akihito , Imai Shizuka , Satake Shoya , Chiba Kouji , Yasuda Makoto 
Published: May 2023

AbstractCumulenes have attracted considerable attention due to their unique structural and electronic properties. Despite their high potential for constructing condensed π‐conjugated molecules, the synthetic utility of longer cumulenes remains to be established owing to their inherently high reactivity. Conjugated bisbutatrienes having two cumulene moieties linked by a spacer as a mimic of a longer cumulene were evaluated. Here, the synthesis and characterization of hexafluorocyclopentane‐bridged bisbutatrienes are described. These bisbutatrienes underwent various cyclizations, to construct the unique π‐extended frameworks inaccessible by other methodologies. The bisbutatrienes were converted into fulvenes, pentalene, germacycle, and benzocyclobutene under various conditions. Furthermore, a cyclooctatetraene derivative was synthesized by a one‐step dimerization of the bisbutatriene.
Infrared and vibrational circular dichroism spectra of methyl β‐D‐glucopyranose in water: The application of the quantum cluster growth and clusters‐in‐a‐liquid solvation models
Authors: Perera Angelo Shehan , Carlson Colton D. , Cheramy Joseph , Xu Yunjie 
Published: May 2023

AbstractThe infrared (IR) and vibrational circular dichroism (VCD) spectra of methyl β‐D‐glucopyranose in water were measured. Both implicit and explicit solvation models were utilized to explain the observed spectra. The vast body of existing experimental and theoretical data suggested that about eight explicit water molecules are needed to account for the solvent effects, supported by the current Quantum Cluster Growth (QCG) analysis. Extensive manual and systematic conformational searches of the molecular target and its water clusters were carried out by using a recently developed conformational searching tool, conformer‐rotamer ensemble sampling tool (CREST), and the microsolvation model in the associated QCG code. The Boltzmann averaged IR and VCD spectra of the methyl β‐D‐glucopyranose‐(water)n (n = 8) conformers in the PCM of water provide better agreement with the experimental ones than those with n = 0, 1, and 2. The explicit solvation with eight water molecules was shown to greatly modify the conformational preference of methyl β‐D‐glucopyranose from its monomeric form. Further analyses show that the result is consistent with the existence of long‐lived methyl β‐D‐glucopyranose monohydrates with the additional explicit water effects being accounted for with the quantum mechanical treatment of the other seven close‐by water molecules in the PCM of water.
Automated Evaluation of Biochemical Plant KPIs based on DEXPI Information
Authors: Klose Anselm , Wagner-Stürz David , Neuendorf Laura , Oeing Jonas , Khaydarov Valentin , Schleehahn Michael , Kockmann Norbert , Urbas Leon 
Published: May 2023

AbstractImplementation of data‐based key performance indicators (KPIs) within existing plants usually requires a thorough manual analysis of the plant topology to define which sensors could provide reliable information for calculating the KPIs. In this publication, we propose an assistance system that automates this process. First the automatic linkage between plant structure and operational data is presented for the evaluation of the process efficiency. Next, a concept and prototypical implementation of the evaluation of predefined KPIs is described. The plant structure is described according to the DEPXI specification, while a rule‐based framework was developed to depict the process‐engineering knowledge. The proposed methods have been validated in an experimental setup. It could be shown that with standardized inputs, a generic extendable evaluation of the plant performance can be achieved.
Supramolecular Surface Engineering of Carbon Dots Enables Matrix‐Free Room Temperature Phosphorescence†
Authors: Zhang Tao , Wu Xiaoyan , Liu Lele , Yang Yuxiang , Zhuang Jialuo , Li Zijian , Bi Hong 
Published: May 2023

Comprehensive SummaryCarbon dots (CDs) are an emerging class of nanomaterials with intriguing photophysical properties. Recently, achieving room temperature phosphorescence (RTP) for CDs has attracted considerable attention for biomedical and information applications. However, the CDs based RTP materials generally require the use of polymeric and inorganic matrix to provide the rigid environments, which remains a great challenge to obtain matrix‐free CDs with RTP. Herein, a novel supramolecular strategy based on strong interparticle interactions has been developed to attain this objective, by covalent decoration of ureido‐pyrimidinone (UPy, a multiple hydrogen bonding unit) on the surface of CDs. Structural characterizations validated the core‐shell structure of the as‐prepared CDs (EDTA‐CDs) and demonstrated the successful attachment of UPy via post‐modification (UPy‐CDs). The presence of UPy recognition units render the strong hydrogen bonding between UPy‐CDs, which stabilizes the triplet state via rigidifying effect. As a result, UPy‐CDs exhibit matrix‐free efficient RTP (λem = 534 nm) with high brightness and long lifetime (33.6 ms) in the solid state. Owing to the dual‐emission character, we further explored the application potential of UPy‐CDs in information encryption and anti‐counterfeiting. Overall, this work provides a new and facile strategy for achieving matrix‐free phosphorescent CDs with elegant incorporation of supramolecular chemistry.
Preclinical Models of Neuroendocrine Prostate Cancer
Authors: Cacciatore Alessia , Albino Domenico , Catapano Carlo V. , Carbone Giuseppina M. 
Published: May 2023

AbstractProstate cancer (PCa) is the most common malignancy and the second leading cause of cancer‐related death amongst men in the United States. Neuroendocrine prostate cancer (NEPC) can either arise de novo or emerge as a consequence of therapy. De novo NEPC is rare, with an incidence of <2% of all PCa cases. In contrast, treatment‐induced NEPC is frequent with >20% of patients with metastatic castration‐resistant prostate cancer (CRPC) reported to progress to neuroendocrine (NE) differentiation. The emergence of treatment‐induced NEPC is linked to the increased therapeutic pressure, due to the broad application of androgen deprivation therapy (ADT) for PCa management and the development of novel more potent androgen receptor (AR) pathway inhibitors. NEPC is a high‐grade tumor type characterized by aggressive phenotype and clinical behavior. Patients affected by NEPC frequently develop visceral metastases and have a poor prognosis. The molecular mechanisms underlying the development and progression of NEPC are still poorly understood. Transcriptional and epigenetic reprogramming appears to be involved in NE progression. In this review, we aim to provide a comprehensive view of the available models for NEPC detailing their strengths and limitations. Moreover, we describe novel approaches to expand the repertoire of preclinical models to better study, prevent, or reverse NEPC. The integration of multiple preclinical models along with molecular and omics approaches will provide important insights to understand disease progression and to devise novel therapeutic strategies for the management of NEPC in the near future. © 2023 The Authors. Current Protocols published by Wiley Periodicals LLC.Basic Protocol 1: Generation of organoids starting from the prostate gland of a GEMM or a human PDXBasic Protocol 2: Ex vivo tumor sphere formation
Using 3dRNA/DNA for RNA and DNA 3D Structure Prediction and Evaluation
Authors: Xiong Yiduo , Zhang Yi , Wang Jian , Xiao Yi 
Published: May 2023

AbstractThis article describes how to use 3dRNA/DNA to predict RNA and DNA 3D structures from their sequences and secondary (2D) structures. The predicted RNA and DNA 3D structures can be used to predict or understand their functions and can also be used to find the interactions between the RNA/DNA and other molecules. © 2023 Wiley Periodicals LLC.Basic Protocol: RNA/DNA 3D structure prediction using 3dRNA/DNA web serverAlternate Protocol: Using 3dRNA/DNA and 3dRNAscore locallySupport Protocol: Installing NSP
The presence of doping agents in dietary supplements: A glimpse into the Brazilian situation
Authors: Torres Clarisse L. , de Oliveira Felipe A. G. , Jooris Luis Felipe , Padilha Monica C. , Pereira Henrique M. G. 
Published: May 2023

AbstractDietary supplements (DS) are intended for healthy people to maintain or improve their overall health. Its consumption is widespread in large part of the general population and at all levels of athletes. Nevertheless, DS use can also pose health risks to individuals and, in the case of athletes, may lead to adverse analytical findings (AAFs) due to the possibility of DS contamination or adulteration with doping agents banned by the World Anti‐Doping Agency. Although educational initiatives are being performed in Brazil to warn the sports community about inadvertent doping cases, AAFs connected to the DS administration have been increasingly growing. The findings of DS analyzed by the Brazilian Doping Control Laboratory (LBCD), between 2017 and 2022, after Testing Authorities (TAs) analysis requests, showed an alarming number of tainted samples. Diuretics were the most common adulterants found in all supplement types. However, the profile of prohibited substances in manufactured and compounded dietary supplements (MDS and CDS, respectively) were distinct, with stimulants being most prevalent in MDS and anabolic agents in CDS products. Additionally, MDS samples generally presented higher estimated concentrations of banned substances (mg/g) than CDS samples (μg/g). The common practice of DS intake by athletes continues to be of great concern for a doping‐free sport, given the high prevalence of prohibited substances detected in the analyzed samples by the LBCD. The current Brazilian scenario reinforces the importance of raising awareness in the sports community of the possible consequences of an unintentional doping case linked to DS use.
Investigating the factor structure and measurement invariance of the Eating Disorder Examination‐Questionnaire (EDE‐Q) in a community sample of gender minority adults from the United States
Authors: Nagata Jason M. , Compte Emilio J. , McGuire F. Hunter , Brown Tiffany A. , Lavender Jason M. , Murray Stuart B. , Capriotti Matthew R. , Flentje Annesa , Lubensky Micah E. , Lunn Mitchell R. , Obedin‐Maliver Juno 
Published: May 2023

AbstractObjectiveThe Eating Disorder Examination‐Questionnaire (EDE‐Q) is one of the most widely used self‐report assessments of eating disorder symptoms. However, evidence indicates potential problems with its original factor structure and associated psychometric properties in a variety of populations, including gender minority populations. The aim of the current investigation was to explore several previously published EDE‐Q factor structures and to examine internal consistency and measurement invariance of the best‐fitting EDE‐Q model in a large community sample of gender minority adults.MethodsData were drawn from 1567 adults (337 transgender men, 180 transgender women, and 1050 gender‐expansive individuals) who participated in The PRIDE Study, a large‐scale longitudinal cohort study of sexual and gender minorities from the United States. A series of confirmatory factor analyses (CFAs) were conducted to explore the fit of eight proposed EDE‐Q models; internal consistency (Cronbach's alphas, Omega coefficients) and measurement invariance (multi‐group CFA) were subsequently evaluated.ResultsA brief seven‐item, three‐factor (dietary restraint, shape/weight overvaluation, body dissatisfaction) model of the EDE‐Q consistently evidenced the best fit across gender minority groups (transgender men, transgender women, gender‐expansive individuals). The internal consistencies of the three subscales were adequate in all groups, and measurement invariance across the groups was supported.DiscussionTaken together, these findings support the use of the seven‐item, three‐factor version of the EDE‐Q for assessing eating disorder symptomatology in gender minority populations. Future studies can confirm the current findings in focused examinations of the seven‐item, three‐factor EDE‐Q in diverse gender minority samples across race, ethnicity, socioeconomic status, and age ranges.Public Significance StatementAlthough transgender individuals have greater risk of developing an eating disorder, the factor structure of the Eating Disorder Examination‐Questionnaire, one of the most widely used eating disorder assessment measures, has not been explored in transgender adults. We found that a seven‐item model including three factors of dietary restraint, shape and weight overvaluation, and body dissatisfaction had the best fit among transgender and nonbinary adults.