bluuebunny/crossref_metadata_2025_split huggingface dataset:
Laparoscopic assisted sclerotherapy in pediatric intra-abdominal cystic lymphatic malformations
Authors: Shi Hao , Lv Zhibao , Xu Weijue , Liu Jiangbin , Sheng Qingfeng , Ren Xiang , Chen Zhou 
Published: April 2023

AbstractBackgroundSclerotherapy is an effective treatment for CLMs of the head and neck. However, Management of intra-abdominal cystic lymphatic malformations(CLMs) remains debates. The purpose of this study was to examine safety and efficacy of laparoscopic assisted sclerotherapy for intra-abdominal CLMs in pediatric patients.MethodsWe retrospectively reviewed the patients treated with laparoscopic assisted sclerotherapy for intra-abdominal CLMs at a single tertiary medical center between July 2020 and July 2022.The basic information and clinical data of maximal diameter, dose, reduction of lesion’s volume and complications were analyzed. The efficacy was assessed by comparing the pre-operative and post-operative cross-sectional images.Results7 patients were included in this study and underwent a total of 9 sclerotherapy sessions. 4 patients were symptomatic and majored in abdominal pain. The primary sclerotherapy did not response in better clinical outcome in 2 patients due to intra-cystic hemorrhage and additional procedure were performed. Lesions in 6 patients reduced ≥90% in volume which met the complete response criteria. No further complications were recorded during the follow-up.ConclusionsLaparoscopic assisted sclerotherapy is a safe and effective method of managing intra-abdominal CLMs. Laparoscopy could localize the origin of lesions and provides more accuracy and stabilization during the injection procedure.
Re-Irradiation Combined with Bevacizumab for Recurrent Glioblastoma beyond Bevacizumab Failure: Survival Outcomes and Prognostic Factors
Authors: You Weir-Chiang , Lee Hsu-Dung , Pan Hung-Chuan , Chen Hung-Chieh 
Published: April 2023

Abstract
Purpose
The combination of re-irradiation and bevacizumab has emerged as a potential therapeutic strategy for patients experiencing their first glioblastoma multiforme (GBM) recurrence. This study aims to assess the effectiveness of the re-irradiation and bevacizumab combination in treating second-progression GBM patients who are resistant to bevacizumab monotherapy.
Methods and materials
This retrospective study enrolled 64 patients who developed a second progression after single-agent bevacizumab therapy. The patients were divided into two groups: 35 underwent best supportive care (none-ReRT group), and 29 received bevacizumab and re-irradiation (ReRT group). The study measured the overall survival time after bevacizumab failure (OST-BF) and re-irradiation (OST-RT). Statistical tests were used to compare categorical variables, evaluate the difference in recurrence patterns between the two groups, and identify optimal cutoff points for re-irradiation volume.
Results
The results of the Kaplan-Meier survival analysis indicated that the re-irradiation (ReRT) group experienced a significantly higher survival rate and longer median survival time than the non-ReRT group. The median OST-BF and OST-RT were 14.5 months and 8.8 months, respectively, for the ReRT group, while the OST-BF for the none-ReRT group was 3.9 months (p < 0.001). The multivariable analysis identified the re-irradiation target volume as a significant factor for OST-RT. Moreover, the re-irradiation target volume exhibited excellent discriminatory ability in the area under the curve (AUC) analysis, with an optimal cutoff point of greater than 27.58 ml.
Conclusions
These findings suggest that incorporating re-irradiation with bevacizumab therapy may be a promising treatment strategy for patients with recurrent GBM resistant to bevacizumab monotherapy. The re-irradiation target volume may serve as a valuable selection factor in determining which patients with recurrent GBM are likely to benefit from the combined re-irradiation and bevacizumab treatment modality.
The receptor for advanced glycation end products is involved in Campylobacter jejuni cytolethal distending toxin intracellular trafficking
Authors: Lai Chih-Ho , Lai Yi-Ru , Chang Yu-Fang , Huang Yi-Ping , Chou Chia-Huei , Ho Mao-Wang , Chiu Cheng-Hsun 
Published: April 2023

Abstract
Cytolethal distending toxin (CDT) belongs to the genotoxin family and is closely related to Campylobacter jejuni-induced gastroenteritis. We recently reported that CDT triggers the danger-associated molecular pattern (DAMP)-associated signaling to exert deleterious effects on host cells. However, how CDT traffics in cells and the mechanism of CDT toxicity remain to be elucidated. In this study, we first reported that CDT interacts with receptor of advanced glycation end product (RAGE) and high mobility group box 1 (HMGB1) to enter the cells. Our results further indicate that CdtB transportation in cells through the dynamin-dependent endocytic pathway and lysosomes is involved in this process. Conversely, knockdown of RAGE using CRISPR-Cas9 resulted in a significant reduction in CDT-arrested cell cycles, indicating that RAGE is essential for CDT action in cells. The results of this study provide valuable insights into the pathogenic potential of CDT-producing bacteria, such as C. jejuni.
Ultrathin Carboxymethyl Cellulose/ Fish Scale Collagen Based Hydrogel Films: Vitamin E Loading, Controlled Release, Antibacterial, and Antioxidant Activities
Authors: Zallaghi Mahsa , Esmkhani Maryam , Javanshir Shahrzad 
Published: April 2023

AbstractCollagen-based hydrogels as scaffolds are one of the most widespread biological materials used in biomedicine and drug carriers. The aim of the current study was to develop a vitamin E (VE) loaded hydrogel films based on fish scale collagen (FSC) and carboxymethyl cellulose crosslinked with triethanolamine (TEA) to evaluate their Vitamin E release efficiency, antibacterial and antioxidant properties. The effect of different collagen content on film properties was investigated. The physical and morphological properties of the extracted FSC and the films were determined different analytical technics. The in-vitro properties of the films, the loading and releasing of VE showed that the highest amount of VE loading (98.1%) and release (92.3%) was achieved for the 14.1 wt% collagen film. The antibacterial assay of the prepared film againstE. coliandS. aureusindicated a 2log reduction after 24 h, corresponding to 99% percent reduction. Based on DPPH radical scavenging activity, FSC/CMC/TEA/VE film showed scavenging activity when compared with standard Vitamin C and FSC/CMC/TEA. Moreover, according to the results of MTT test, the film has no toxic effect. Based on these results, the prepared hydrogel film seems to be a promising option in the field of drug delivery.
Regulatory T cell-associated signature: predicting prognosis and immunotherapy in ovarian cancer
Authors: Ji Yaya , Liu Jinhui , Sun Ying , Wang Fengxu , Xia Weiyi , Wang Xiaoke , Zhao Xinyuan , Zhang Pengpeng , Luo Yonghua , Yu Shali 
Published: April 2023

Abstract
Background Immunosuppression is a key feature of cancer, promoting tumor growth and progression. Regulatory T cells (Tregs) are a specialized subset of T lymphocytes that mediate immunosuppression in cancer. However, the role of Treg-related genes in ovarian cancer (OC) remains largely unexplored.Methods To identify marker genes related to Tregs in OC, we utilized scRNA-seq analysis. Treg scores were then constructed using single-sample gene set enrichment analysis (ssGSEA) based on these markers. To identify gene modules with the strongest correlation with Treg scores in bulk RNA-seq data, we employed the Weighted Gene Co-expression Network Analysis (WGCNA) algorithm. Multiple machine learning algorithms were then used to construct risk models with superior predictive performance, which were validated using external independent datasets. A risk score was developed for each OC sample based on the optimal model to evaluate differences in prognosis, immune infiltration, pathway activity, and immunotherapy between high and low-risk groups.Results We identified 365 genes regulating Treg activity using the WGCNA algorithm, and found that 70 of these genes were associated with the prognosis of OC based on univariate cox analysis. Using a combination of the Random Survival Forest (RSF) and Lasso algorithms, we constructed a risk model with the highest c-index based on the assigned risk scores. The model's performance was validated using four external datasets. Our analysis revealed that the low-risk group had a better prognosis, more immune cell infiltration, higher immune checkpoint expression, and significant differences in pathway enrichment and immunotherapy efficacy between the different risk groups.Conclusion Our findings provide new insights into the role of Treg cells in the development and progression of OC and highlight the potential for developing novel Treg-targeted therapies for the treatment of this disease.
Assessment of brain tumour perfusion using early-phase 18F-FET -PET: comparison with perfusion-weighted MRI
Authors: Filss Christian P. , Cramer Julian , Löher Saskia , Lohmann Philipp , Stoffels Gabriele , Stegmayr Carina , Kocher Martin , Heinzel Alexander , Galldiks Norbert , Wittsack Hans J. , Sabel Michael , Neumaier Bernd , Scheins Jürgen , Shah N. Jon , Meyer Philipp T. , Mottaghy Felix M. , Langen Karl-Josef 
Published: April 2023

Abstract
Background: Morphological imaging using MRI is essential for brain tumour diagnostics. Dynamic susceptibility contrast (DSC) perfusion-weighted MRI (PWI), as well as amino acid PET, may provide additional information in ambiguous cases. Since PWI is not always performed as part of standard MRI in brain tumours, we explored whether maps of relative cerebral blood volume (rCBV) in brain tumours can be extracted from the early phase of PET using O-(2-18F-fluoroethyl)-L-tyrosine (18F-FET).
Using a hybrid BrainPET/MRI scanner, PWI and dynamic 18F-FET PET were performed in 33 patients with cerebral glioma and in four patients with highly vascularized meningiomas. Based on the dynamic PET data in meningiomas, the time interval from 0 – 2 min p.i. was selected to best reflect the blood pool phase in 18F-FET PET. For each patient, maps of MR-rCBV, early 18F-FET PET (0-2 min p.i.) and late 18F-FET PET (20-40 min p.i.) were generated and coregistered. Volumes of interest were placed on the tumour (VOI-TU) and on the normal-appearing contralateral brain tissue (VOI-REF). The correlation between the tumour-to-brain ratios (TBR) of the different parameters was analyzsed. In addition, three independent observers evaluated the MR-rCBV and early 18F-FET maps (18F-FET-rCBV) for concordance in signal intensity, tumour extent and intratumoural distribution.
Results: TBRs calculated from MR-rCBV and 18F-FET-rCBV showed a significant correlation (r = 0.89, p < 0.001), while there was no correlation between late 18F-FET PET and MR-rCBV (r = 0.24, p = 0.16) or 18F-FET-rCBV (r = 0.27, p = 0.11). Visual rating yielded widely agreeing findings or only minor differences between the MR-rCBV maps and 18F-FET-rCBV maps in 93 % of the tumours (range of three independent raters 91–94%, kappa among raters 0.78-1.0).
Conclusion: Early 18F-FET-maps (0-2min p.i.) in gliomas provide similar information to MR-rCBV maps and may be helpful when PWI is not possible or available. Further studies in recurrent gliomas are needed to evaluate whether 18F-FET-rCBV provides the same clinical information as MR-rCBV.
Effect of Interpersonal Effectiveness Skills Training Intervention on Interpersonal Communication Competence and Social Functioning among Patients with Depression
Authors: atta mohamed , ghazi ghada 
Published: April 2023

Abstract
Background Study group (21 male and 9 female) of adult patients with depression were offered a three-month interpersonal effectiveness skill training.Objective Determine the effect of implementing interpersonal effectiveness skills training intervention on interpersonal communication competence and social functioning among patients with depression than those who didn’t receive such intervention. Design of study: A quasi-experimental research design. Settings: The study was conducted in psychiatric outpatient clinics of the Main University Hospital of Alexandria University. Subjects: 30 patients with depression for applying the interpersonal effectiveness training intervention and30 patients with depression for control group. Tools: Three tools were used to collect the data for this study; socio-demographic and clinical data structured interview schedule, interpersonal communication competence scale (ICCS) and social functioning scale (SFS).Results There are statistically significance differences in ICCS and SFS in total and subscales scores among study group before and after conducting interpersonal effectiveness skills intervention and vice versa is true for control group.Conclusion Patients with depression can be enhanced through interpersonal effectiveness skill training. Recommendations: Interpersonal effectiveness skills training is recommended for patients with depression as well as pharmacological treatment.
mTOR inhibition alleviates CD8+ T-cell senescence in activated phosphoinositide 3-kinase δ syndrome 2 patients
Authors: Han Lingli , Liu Luyao , Zhao Qifan , Bu Huaqin , Wang Wenjie , Sun Bijun , Ying Wenjing , Hui Xiaoying , Yao Haili , Hou Jia , Wang Xiaochuan , Wang Ying , Lu Wei , Sun Jinqiao 
Published: April 2023

Abstract
Background We investigated the clinical and immunological features in a Chinese cohort of activated phosphatidylinositol 3-kinase δ syndrome 2 (APDS2) and assessed the efficacy of Rapamycin therapy and the underlying mechanism.Results The shared clinical manifestation of patients included recurrent respiratory tract infection, lymphadenopathy, persistent or recurrent splenomegaly, and hepatomegaly. Three patients carry PIK3R1 c.1425 + 1G > A mutation, and one patient has the mutation c.1425 + 2T > G. Patients have defective humoral immunity with decreased B lymphocytes, especially memory B cells, and suffered from decreased naïve T cells and elevated senescent CD8+ T cells. Two patients after rapamycin therapy showed improved clinical symptoms. They also have decreased CD8+ effector memory T cells and terminal effector memory cytotoxic T cells. TCF1 was downregulated in CD8+ T cells of PIK3R1 patients but upregulated after Rapamycin treatment, which was correlated with decreased senescent CD8+ T cells.Conclusions mTOR inhibitor rapamycin improved clinical symptoms in APDS2 patients and reversed CD8+ T cell senescence through TCF1-dependent signal pathway.
Associations between anthropometric markers derived from a body scanner and relative fat-free mass in a population-based study
Authors: Dietzmann Maximilian , Radke Dörte , Markus Marcello RP , Völzke Henry , Felix Stephan B. , Dörr Marcus , Bahls Martin , Ittermann Till 
Published: April 2023

Abstract
Low relative fat free mass (FFM) is associated with an increased risk of chronic diseases and mortality, but FFM assessment in clinical routine is time consuming and currently not performed. Our aim was to identify easily accessible anthropometric markers for FFM derived by either bioelectrical impedance analysis (BIA) or air displacement plethysmography (ADP). We analyzed data of 1,593 individuals (784 women; 49.2%, age range 28 – 88 years) enrolled in the population-based Study of Health in Pomerania (SHIP-TREND 1). Forty-seven anthropometric markers were derived from a body scanner. Anthropometric measurements were associated with FFM by sex-stratified linear regression models adjusted for body height and age. Anthropometric markers were ranked according to the coefficient of determination (R2) derived from these regression models. Circumferences of high hip, belly, middle hip, waist and high waist showed the strongest inverse associations with FFM. These relations were stronger in females than in males. Associations of anthropometric markers with FFM measured by ADP were stronger compared to FFM assessment by BIA. Manually measured waist and hip circumference showed slightly weaker associations with the FFM compared to the automated measurements. The detected anthropometrics could be helpful in assessing FFM in clinical routine.
Accuracy in osteotomy drilling using a new universal and disposable drill-stop device for dental implant drills: an in vitro study using a bovine rib model
Authors: Camacho-Alonso Fabio , Pérez-Sayáns Mario , Pato-Mourelo Jesús , Martínez-Martínez Juan Enrique 
Published: April 2023

Abstract
Objective To evaluate the surgical accuracy of a new universal disposable stop system for implant drills (FCA Universal Drill Stop).Materials and Methods A total of 60 bovine ribs were included in this in vitro study. The ribs were randomized into three study groups (n = 20 ribs per group). In each study group (Group1: drills without stop or control group, Group 2: prefabricated drills with stop or gold standard group, and Group 3: drills with FCA Universal Drill Stop) a total of 100 osteotomies were performed with implant drills in each group, following the drilling sequence for the placement of a dental implant of 10 mm length and 4 mm diameter. The accuracy of the depth of the osteotomies was quantified clinically (with periodontal probe) and radiologically, using ImageJ version 1.48v software.Results The order of highest to lowest accuracy (clinical and radiological) in the depth of osteotomies was: FCA Universal Drill Stop > prefabricated drills with a stop > drills without stop, with statistically significant differences being observed between both systems with stop with respect to the control group, although not between them.Conclusions The new universal disposable stop system for implant drills, offers similar accuracy to prefabricated drills with stop, with both systems being much more accurate than implant drills without stop. Clinical relevance: Our results indicate that the new FCA Universal Drill Stop system is an accurate device for implant osteotomies, offering the advantage of being a universal system for all brands of dental implants, but further clinical studies are necessary.
The common mycorrhizal network originated from Brachiaria brizantha suppresses the growth of seedlings from the grass itself and native trees in shouthern Brazil
Authors: Bertagnoli Breynner G. Pavão , Filho Arnaldo Colozzi , Pimenta José Antonio , Zangaro Waldemar 
Published: April 2023

Abstract
The common mycorrhizal network (CMN) is formed by arbuscular mycorrhizal fungi (AMF) that interconnect plant roots, and promote the exchange of nutrients, water, and signaling compounds, in addition to serving as a mechanism of competition among plants. It was evaluated the influence of CMN, originated from the adult invasive grass Brachiaria brizantha, on the development of seedlings of B. brizantha itself and of the native trees Heliocarpus popayanensis (early-successional) and Cariniana estrellensis (late-successional). The plants were grown in a substrate composed of fertile soil and sterilized sand in a 3:1 ratio, with 640 native AMF spores per gram of soil. The treatments were: 1) Seedlings of B. brizantha, H. popayanensis, and C. estrellensis grew under the influence of CMN originated from adult B. brizantha for 225 days; 2) Ninety days after the influence of CMN, two adult B. brizantha prunings were carried out, the first occurring 90 days after emergence and the second 180 days after the first pruning; 3) Ninety days after the influence of the CMN, the communication network was severed by the weekly rupture of the fungal hyphae; 4) The control treatment consisted of the isolated growth of five seedlings of each species without contact with the CMN. The CMN originated from B. brizantha suppressed the growth of seedlings of the three species. CMN severing completely eliminated the suppressive effects of adult B. brizantha on seedlings. The adult B. brizanta pruning mitigated the negative effects on seedlings, however after regrowth, suppression returned. The seedlings of H. popayanensis connected to the CMN originated from adult B. brizantha died, while the seedlings of C. estrellensis and B. brizantha itself survived, altough suppressed.
The long-term effect on surgery-free survival of biological compared to conventional therapy in Crohn’s disease in real world-data: a propensity-score matched study
Authors: Valvano Marco , Vinci Antonio , Cesaro Nicola , Frassino Sara , Ingravalle Fabio , Ameli Marta , Viscido Angelo , Necozione Stefano , Latella Giovanni 
Published: April 2023

Abstract
BACKGROUND: The introduction of biological drugs has led to great expectations and growing optimism in the possibility that this new therapeutic strategy could favourably change the natural history of Inflammatory Bowel Disease (IBD) and in particular that it could lead to a significant reduction in surgery in the short and long term. The evaluation of the incidence of intestinal resection after the introduction of biological therapy is very complex and subject to several potential bias, the most prominent being the shift in IBD management over the last few decades.
This study aims to assess the impact of biological versus conventional therapy on surgery-free survival time (from the diagnosis to the first bowel resection) and on the overall risk of surgery in patients with Crohn’s disease (CD) who were never with the surgical option.
METHODS: This is a retrospective, double-arm study including CD patients treated with either biological or conventional therapy (mesalamine, immunomodulators, antibiotics, or steroids). All CD patients admitted at the GI Unit of the S. Salvatore Hospital (L’Aquila. Italy) and treated with biological therapy since 1998 were included in the biological arm. Data concerning the CD patients receiving a conventional therapy were retrospectively collected from our database. These patients were divided into a pre-1998 and post-1998 group. Our primary outcome was the evaluation of the surgery-free survival since CD diagnosis to the first bowel resection. Surgery-free time and event incidence rates were calculated and compared among all groups, both in the original population and in the propensity-matched population.
RESULTS: 203 CD patients (49 biological, 93 conventional post-1998, 61 conventional pre-1998) were included in the study. Kaplan-Mayer survivorship estimate shows that patients in the biological arm had a longer surgery-free survival compared to those in the conventional arm (p=0.03). However, after propensity matching analysis, no significant difference was found in surgery-free survival (p=0.3). A sub-group analysis showed shorter surgery-free survival in patients on conventional therapy in the pre-biologic era only (p=0.02) while no significative difference was found between the biologic and conventional post-biologic groups (p=0.15).
CONCLUSION: This study shows that the introduction of biological therapy had only a slight impact on the occurrence of surgery in CD patients over a long observation period. Nevertheless, biological therapy appears to delay the first intestinal resection.