bluuebunny/crossref_metadata_2025_split huggingface dataset:
Climate change threatens unique evolutionary diversity in Australian kelp refugia
Authors: Nimbs Matt J. , Wernberg Thomas , Davis Tom R. , Champion Curtis , Coleman Melinda A. 
Published: January 2023

AbstractClimate change has driven contemporary decline and loss of kelp forests globally with an accompanying loss of their ecological and economic values. Kelp populations at equatorward-range edges are particularly vulnerable to climate change as these locations are undergoing warming at or beyond thermal tolerance thresholds. Concerningly, these range-edge populations may contain unique adaptive or evolutionary genetic diversity that is vulnerable to warming. We explore haplotype diversity by generating a Templeton–Crandall–Sing (TCS) network analysis of 119 Cytochrome C Oxidase (COI) sequences among four major population groupings for extant and putatively extinct populations only known from herbarium specimens of the dominant Laminarian kelp Ecklonia radiata in the south-western Pacific, a region warming at 2–4 times the global average. Six haplotypes occurred across the region with one being widespread across most populations. Three unique haplotypes were found in a deep-water range-edge population off Moreton Island, Queensland, which likely represents both a contemporary and historic refuge during periods of climatic change. Hindcasting E. radiata cover estimates using extant data, we reveal that this region likely supported the highest kelp cover in eastern Australia during the last glacial maximum. The equatorward range edge, deep-water kelp populations off Moreton Island represent a genetically diverse evolutionary refuge that is currently threatened by warming and requires prompt ex-situ conservation measures.
Botanical inhibitors of SARS-CoV-2 viral entry: a phylogenetic perspective
Authors: Risener Caitlin J. , Woo Sunmin , Samarakoon Tharanga , Caputo Marco , Edwards Emily , Klepzig Kier , Applequist Wendy , Zandi Keivan , Goh Shu Ling , Downs-Bowen Jessica A. , Schinazi Raymond F. , Quave Cassandra L. 
Published: January 2023

AbstractThroughout the SARS-CoV-2 pandemic, the use of botanical dietary supplements in the United States has increased, yet their safety and efficacy against COVID-19 remains underexplored. The Quave Natural Product Library is a phylogenetically diverse collection of botanical and fungal natural product extracts including popular supplement ingredients. Evaluation of 1867 extracts and 18 compounds for virus spike protein binding to host cell ACE2 receptors in a SARS-CoV-2 pseudotyped virus system identified 310 extracts derived from 188 species across 76 families (3 fungi, 73 plants) that exhibited ≥ 50% viral entry inhibition activity at 20 µg/mL. Extracts exhibiting mammalian cytotoxicity > 15% and those containing cardiotoxic cardiac glycosides were eliminated. Three extracts were selected for further testing against four pseudotyped variants and infectious SARS-CoV-2 and were then further chemically characterized, revealing the potent (EC50 < 5 µg/mL) antiviral activity of Solidago altissima L. (Asteraceae) flowers and Pteridium aquilinum (L.) Kuhn (Dennstaedtiaceae) rhizomes.
Immunotherapy targeting the PD-1 pathway alleviates neuroinflammation caused by chronic Toxoplasma infection
Authors: Xiao Jianchun , Li Ye , Rowley Treva , Huang Jing , Yolken Robert H. , Viscidi Raphael P. 
Published: January 2023

AbstractToxoplasma gondii can infect the host brain and trigger neuroinflammation. Such neuroinflammation might persist for years if the infection is not resolved, resulting in harmful outcomes for the brain. We have previously demonstrated the efficacy of immunotherapy targeting the programmed cell death protein 1 (PD-1) pathway on clearance of Toxoplasma tissue cysts. We aimed to test whether parasite clearance would lead to the resolution of neuroinflammation in infected brains. We established chronic Toxoplasma infection in BALB/c mice using the cyst-forming Prugniaud strain. Mice then received αPD-L1 or isotype control antibodies. After completion of the therapy, mice were euthanized six weeks later. The number of brain tissue cysts, Toxoplasma-specific CD8 + T cell proliferation and IFN-γ secretion, serum cytokine and chemokine levels, and CNS inflammation were measured. In αPD-L1-treated mice, we observed reduced brain tissue cysts, increased spleen weight, elevated IFN-γ production by antigen-specific CD8 + T cells, and a general increase in multiple serum cytokines and chemokines. Importantly, αPD-L1-treated mice displayed attenuation of meningeal lymphocytes, reactive astrocytes, and C1q expression. The reduction in inflammation-related proteins is correlated with reduced parasite burden. These results suggest that promoting systemic immunity results in parasite clearance, which in turn alleviates neuroinflammation. Our study may have implications for some brain infections where neuroinflammation is a critical component.
A computational framework for physics-informed symbolic regression with straightforward integration of domain knowledge
Authors: Keren Liron Simon , Liberzon Alex , Lazebnik Teddy 
Published: January 2023

AbstractDiscovering a meaningful symbolic expression that explains experimental data is a fundamental challenge in many scientific fields. We present a novel, open-source computational framework calledScientist-Machine Equation Detector(SciMED), which integrates scientific discipline wisdom in a scientist-in-the-loop approach, with state-of-the-art symbolic regression (SR) methods. SciMED combines a wrapper selection method, that is based on a genetic algorithm, with automatic machine learning and two levels of SR methods. We test SciMED on five configurations of a settling sphere, with and without aerodynamic non-linear drag force, and with excessive noise in the measurements. We show that SciMED is sufficiently robust to discover the correct physically meaningful symbolic expressions from the data, and demonstrate how the integration of domain knowledge enhances its performance. Our results indicate better performance on these tasks than the state-of-the-art SR software packages , even in cases where no knowledge is integrated. Moreover, we demonstrate how SciMED can alert the user about possible missing features, unlike the majority of current SR systems.
An eye- tracking technology and MLP-based color matching design method
Authors: Hua Yinhong , Ni Jianlin , Lu Honglei 
Published: January 2023

AbstractImages are a significant source of inspiration for designers to carry out the color design. However, the absence of animated images in the product color design can create confusion for designers. To translate the colours of the animated images into product colours, this work used eye-tracking technology to aid colour extraction and the multilayer perceptron neural network (MLP) algorithm to train a product colour decision model to filter the best product colour schemes. Firstly, eye tracking technology is used to collect the distribution of hotspots of the subject while viewing the animated images. Based on the distribution of eye-tracking hotspots, the most interesting animated colours were extracted. Then, the MLP is applied to train a colour decision model for children's shopping cart products, and the colour decision model is used to filter the optimal solution for the product colour, and finally the colour design is completed from the animated colour to the three-colour children's shopping cart product. Experimental results show that the color extraction based on the eye-tracking technology and the color scheme screening based on the intelligent algorithm can realize the effective conversion from animated image colors to product colors. This work proposes a color scheme design method from animations to products, which further expands the image color sources in product color design and can accurately find the color scheme that matches the animated image and the product.
Bidirectional dispersals during the peopling of the North American Arctic
Authors: Luis Javier Rodriguez , Palencia-Madrid Leire , Garcia-Bertrand Ralph , Herrera Rene J. 
Published: January 2023

AbstractIt is thought that Paleo-Inuit were the first people that settled the American Arctic about 5000 BP (before the present) from a migration that crossed Beringia from Northeast Asia. It is theorized that this group initially migrated to the North Slopes of Alaska and subsequently expanded eastward, eventually reaching Greenland. A second circumpolar dispersal of Neo-Inuit from the North Slopes associated with the Thule-Inuk culture has been postulated to have extended eastward around 800 BP, totally replacing the original Paleo-Inuit without admixing. Although generally accepted, this migration scenario is incompatible with previously reported indications of east to west gene flow across the American Arctic. Here we report on the Y-chromosome haplogroup and Y-STR diversity of the four circumpolar populations of the Tuva Republic (N = 24), Northeast Siberia (N = 9), Bethel, Alaska (N = 40), and Barrow, Alaska (N = 31). Four haplogroup lineages (Q-NWT01, Q-M3, Q-M346, and Q-M120) were detected, Q-NWT01 and Q-M3 being the most abundant at 11.11 and 66.67% in Northeast Siberia, 32.50 and 65.00% in Bethel, and 67.74 and 32.26% in Barrow, respectively. The same samples genotyped for Y-chromosome SNPs were typed for 17 Y-STYR loci using the AmpFlSTR Yfiler system. Age estimates and diversity values for the Q-NWT01 and Q-M3 mutations suggest extensive movement of male individuals along the entire longitudinal stretch of the American circumpolar region. Throughout the entire region, Q-M3 exhibits a west to east decreasing gradient in age and diversity while Q-NWT01 indicates the opposite with older TMRCA and higher diversity values running from east to west with the most recent estimates in Canada and Alaska. The high age and diversity values in Greenland are congruent with an origin of the Q-NWT01 mutation in the east of the circumpolar range about 2000–3000 ya. This scenario is incompatible with a complete biological replacement starting about 700 BP of Paleo-Inuit like the Dorset by the Thule-Inuit (Neo-Inuit), as is currently thought, and more parsimonious with gene flow carrying the NWT01 mutation from a pre-Thule population to the ancestors of the present-day Inuit.
Aqueous microRNA profiling in age-related macular degeneration and polypoidal choroidal vasculopathy by next-generation sequencing
Authors: Choi Yeong A. , Jeong Areum , Woo Chang-Hoon , Cha Soon Cheol , Park Do Young , Sagong Min 
Published: January 2023

AbstractAlthough many studies demonstrated the differences of clinical features, natural course, and response to treatment between typical age-related macular degeneration (AMD) and polypoidal choroidal vasculopathy (PCV), differential microRNAs (miRNAs) expression in the aqueous humor (AH) between them has not been reported yet. We investigated the roles of miRNAs in the AH of patients with typical AMD and PCV using next-generation sequencing (NGS) and quantitative PCR (qPCR). Target genes and predicted pathways of miRNAs were investigated via pathway enrichment analysis using the Kyoto Encyclopedia of Genes and Genomes database. A total of 161 miRNAs from eyes with typical AMD and 185 miRNAs from eyes with PCV were differentially expressed. 33 miRNAs were commonly upregulated, and 77 miRNAs were commonly downregulated in both typical AMD and PCV groups. Among them, hsa-miR-140-5p, hsa-miR-374c-3p, and hsa-miR-200a-5p were differentially expressed and were predicted to regulate proteoglycans in cancer, p53 signaling pathway, Hippo signaling pathway, and adherens junction. The differential expression profiles and target gene regulation networks of AH miRNAs may contribute to the development of different pathological phenotypes in typical AMD and PCV. The results of this study provide novel insights into the pathogenesis, associated prognostic biomarkers, and therapeutic targets in AMD and PCV.
Protective effect of urotensin II receptor antagonist urantide and exercise training on doxorubicin-induced cardiotoxicity
Authors: Sun Jing , Zhou Jiedong , Sun Shimin , Lin Hui , Zhang Hanlin , Zhong Zuoquan , Chi Jufang , Guo HangYuan 
Published: January 2023

AbstractDoxorubicin (DOX) has a wide antitumor spectrum, but its adverse cardiotoxicity may lead to heart failure. Urotensin II (UII) is the most potent vasoconstrictor in mammals. It plays a role by activating the UII receptor (UT), the orphan G protein-coupled receptor (GPR14), collectively referred to as the UII/UT system. In the new version of "Chinese expert consensus on cardiac rehabilitation of chronic heart failure," it is pointed out that exercise rehabilitation is the cornerstone of cardiac rehabilitation. In this study, in vitro and in vivo assessments were performed using DOX-treated H9C2 cells and rats. It was found that the UT antagonist Urantide and exercise training improved DOX-induced cardiac insufficiency, reduced DOX-induced cardiomyocyte apoptosis, improved the structural disorder of myocardial fibers, and inhibited DOX-induced myocardial fibrosis. Further studies showed that Urantide alleviated DOX-induced cardiotoxicity by downregulating the expression levels of the p38 mitogen-activated protein kinase signaling pathway.
Exploratory analysis of the effect of a dexamethasone-sparing regimen for prophylaxis of cisplatin-induced emesis on food intake (LUNG-NEPA study)
Authors: Celio Luigi , Cortinovis Diego , Cogoni Alessio Aligi , Cavanna Luigi , Martelli Olga , Carnio Simona , Collovà Elena , Bertolini Federica , Petrelli Fausto , Cassano Alessandra , Chiari Rita , Zanelli Francesca , Pisconti Salvatore , Vittimberga Isabella , Letizia Antonietta , Misino Andrea , Gernone Angela , Bonizzoni Erminio , Pilotto Sara , De Placido Sabino , Bria Emilio 
Published: January 2023

AbstractWe demonstrated the non-inferiority of a dexamethasone (DEX)-sparing (single-dose) regimen with NEPA, a netupitant/palonosetron fixed combination, for preventing chemotherapy-induced nausea and vomiting (CINV) caused by cisplatin. This pre-planned exploratory analysis assessed the effect of the DEX-sparing regimen on a patient’s food intake. Chemotherapy-naïve patients undergoing cisplatin (≥ 70 mg/m2) were given NEPA and DEX (12 mg) on day 1 and randomized to receive either no further DEX (DEX1), or oral DEX (4 mg BID) on days 2–4 (DEX4). Patient-reported endpoint maintenance of usual daily food intake was assessed during the 5-days post-chemotherapy. The relationship between usual daily food intake and CINV control, pre-chemotherapy self-rated food intake and BMI-adjusted weight loss (WL) were evaluated. One-hundred fifty-two patients (76/group) were assessable. The proportion of patients reporting maintenance of usual daily food intake was similar in both groups: 69.7% (95% CI, 58.6–78.9) for DEX1 vs. 72.4% (95% CI, 61.4–81.2) for DEX4. Only CINV control was significantly associated with maintenance of usual daily food intake (P ≤ 0.001) during the overall phase. The DEX-sparing regimen does not adversely affect patient-reported daily food intake post-chemotherapy. The current analysis adds further insights into antiemetic efficacy of DEX sparing beyond day 1 in the challenging setting of cisplatin.Trial registration: The parent study was registered on ClinicalTrials.gov (NCT04201769).
A retrospective analysis of the incidence of severe adverse events among recipients of chiropractic spinal manipulative therapy
Authors: Chu Eric Chun-Pu , Trager Robert J. , Lee Linda Yin-King , Niazi Imran Khan 
Published: January 2023

AbstractThis study examined the incidence and severity of adverse events (AEs) of patients receiving chiropractic spinal manipulative therapy (SMT), with the hypothesis that < 1 per 100,000 SMT sessions results in a grade ≥ 3 (severe) AE. A secondary objective was to examine independent predictors of grade ≥ 3 AEs. We identified patients with SMT-related AEs from January 2017 through August 2022 across 30 chiropractic clinics in Hong Kong. AE data were extracted from a complaint log, including solicited patient surveys, complaints, and clinician reports, and corroborated by medical records. AEs were independently graded 1–5 based on severity (1-mild, 2-moderate, 3-severe, 4-life-threatening, 5-death). Among 960,140 SMT sessions for 54,846 patients, 39 AEs were identified, two were grade 3, both of which were rib fractures occurring in women age > 60 with osteoporosis, while none were grade ≥ 4, yielding an incidence of grade ≥ 3 AEs of 0.21 per 100,000 SMT sessions (95% CI 0.00, 0.56 per 100,000). There were no AEs related to stroke or cauda equina syndrome. The sample size was insufficient to identify predictors of grade ≥ 3 AEs using multiple logistic regression. In this study, severe SMT-related AEs were reassuringly very rare.
Fucosylation of HLA-DRB1 regulates CD4+ T cell-mediated anti-melanoma immunity and enhances immunotherapy efficacy
Authors: Lester Daniel K. , Burton Chase , Gardner Alycia , Innamarato Patrick , Kodumudi Krithika , Liu Qian , Adhikari Emma , Ming Qianqian , Williamson Daniel B. , Frederick Dennie T. , Sharova Tatyana , White Michael G. , Markowitz Joseph , Cao Biwei , Nguyen Jonathan , Johnson Joseph , Beatty Matthew , Mockabee-Macias Andrea , Mercurio Matthew , Watson Gregory , Chen Pei-Ling , McCarthy Susan , MoranSegura Carlos , Messina Jane , Thomas Kerry L. , Darville Lancia , Izumi Victoria , Koomen John M. , Pilon-Thomas Shari A. , Ruffell Brian , Luca Vincent C. , Haltiwanger Robert S. , Wang Xuefeng , Wargo Jennifer A. , Boland Genevieve M. , Lau Eric K. 
Published: January 2023

AbstractImmunotherapy efficacy is limited in melanoma, and combinations of immunotherapies with other modalities have yielded limited improvements but also adverse events requiring cessation of treatment. In addition to ineffective patient stratification, efficacy is impaired by paucity of intratumoral immune cells (itICs); thus, effective strategies to safely increase itICs are needed. We report that dietary administration of l-fucose induces fucosylation and cell surface enrichment of the major histocompatibility complex (MHC)-II protein HLA-DRB1 in melanoma cells, triggering CD4+ T cell-mediated increases in itICs and anti-tumor immunity, enhancing immune checkpoint blockade responses. Melanoma fucosylation and fucosylated HLA-DRB1 associate with intratumoral T cell abundance and anti-programmed cell death protein 1 (PD1) responder status in patient melanoma specimens, suggesting the potential use of melanoma fucosylation as a strategy for stratifying patients for immunotherapies. Our findings demonstrate that fucosylation is a key mediator of anti-tumor immunity and, importantly, suggest that l-fucose is a powerful agent for safely increasing itICs and immunotherapy efficacy in melanoma.
Haptics based multi-level collaborative steering control for automated driving
Authors: Nakade Tomohiro , Fuchs Robert , Bleuler Hannes , Schiffmann Jürg 
Published: January 2023

AbstractIncreasing the capability of automated driving vehicles is motivated by environmental, productivity, and traffic safety benefits. But over-reliance on the automation system is known to cause accidents. The role of the driver cannot be underestimated as it will ultimately be the most relevant aspect for trust building and social acceptance of this technology. Here we introduce a driver-oriented automation strategy to achieve collaborative steering. Our approach relies on three major functionalities: interaction, arbitration, and inclusion. The proposed control strategy is grounded in the concept of shared control enabling driver intervention over the automation without deactivation. Well-defined physical human-robot interaction types are made available with the arbitration strategy. The automated driving trajectory is adapted to include the driver intent into the tactical level of trajectory planning. This enables driver initiated rerouting and consistent coordination of all vehicle actuators. In this way, automated vehicles, which rely on sight only, are augmented with the incorporation of the driver intent. The driver is neither replaced by nor excluded from the automation, rather their role remains active to the benefit of trust building and driving safety.
Pediatric Dysphagia: A Look Into the Training Received During Graduate Speech-Language Pathology Programs to Support This Population
Authors: Knollhoff Stephanie M. 
Published: January 2023

Purpose:
The purpose of this study was to examine academic preparation in pediatric swallowing and feeding, specifically, how speech-language pathology graduate programs were addressing pediatric swallowing and feeding, both academically and clinically, prior to guideline changes and modifications in 2020 due to updates and the global health pandemic.

Method:
To gain a rounded image of speech-language pathology academic programs, two different methodological approaches were utilized. First, courses from 272 accredited programs were analyzed. Second, survey results from 100 participants were analyzed to investigate clinical clock hours and experiences received during participation in a graduate speech-language pathology academic program.

Results:
Less than 1% (0.4%) of academic courses analyzed were categorized as pediatric swallowing and feeding. Greater than 90% of participants reported receiving 20 hr or less in pediatric dysphagia, assessment, or treatment. Sixty percent of participants reported not feeling well prepared at all to provide pediatric swallowing and feeding services.

Conclusions:
There are a growing number of children requiring swallowing and feeding services, a trend that looks to continue its rise. Pediatric dysphagia is a complex area requiring unique knowledge and skills. Academic programs have a responsibility to their students, and the individuals requiring speech-language pathology services, to provide experiences that allow for knowledge and skill development across the entire scope of practice and life span.
A microwave limiter with non‐reciprocal limiting threshold
Authors: Shen Jian , Liu Jibin , Lin Mingtuan , Wu Zhaofeng , Deng Shixiong 
Published: January 2023

AbstractA novel microwave limiter with non‐reciprocal limiting threshold is proposed in this paper to protect the transceiver switch or the transmitter. The directivity of the directional coupler is utilized to make the power of the received signal input to the detection circuit larger than that of the transmitted signal, thereby the detection circuit provides different DC bias voltages to the limiter circuit and changes the threshold level of the limiter diode. The test results show that this limiter has a threshold level of 35 dBm for the transmitted signal and 17 dBm for the received signal, which has a non‐reciprocal limiting threshold for high‐power signals input in both directions.