bluuebunny/crossref_metadata_2025_split huggingface dataset:
A novel t (5; 17) (q35; q21) associated with t (8; 21) (q22; q22) in a patient with acute myeloid leukemia: Case report and review of literature
Authors: Zahra Kmira , Cherif Wided , Ahmed Gereisha , Regaieg Haifa , Nesrine Ben Sayed , Zaier Monia , Mootamri Wided , Youssef Yosra Ben , Brahem Nejia , Sennana Halima , Khelif Abderrahim 
Published: March 2023

Abstract
The t (8; 21) (q22; q22) with the resulting RUNX1- RUNX1T1 rearrangement is one of the most common cytogenetic abnormalities in acute myeloid leukemia (AML). It is associated with favorable prognosis. The t (5; 17) (q35; q21) is an uncommon translocation, fuses the gene for the nucleophosmin (NPM) to the retinoic acid receptor α(RARA) and was described essentially in acute promyelocytic leukemia (APL) variant. We present the case of a 19-year-old male patient who developed an AML with t (8 ;21) (q22 ;q22) associated to t(5 ;17)(q35 ;21). Morphology and immunophenotype of the leukemic cells were compatible with AML. The patient received chemotherapy based on cytarabine and anthracycline without all-trans retinoic acid (ATRA) followed by allogenic stem cells transplantation in first remission. To the best of our knowledge, this is the first report of an association between a rare translocation t (5; 17) and t (8;21) in AML. In this report, we will discuss the prognosis of this association as well as the treatment.
The Impact of Body Mass Index and Medical Conditions on Home-based Anal Self-Sampling
Authors: Nitkowski Jenna , Fernandez Maria E. , Ridolfi Tim , Chiao Elizabeth , Giuliano Anna R. , Schick Vanessa , Swartz Michael D. , Smith Jennifer S. , Nyitray Alan G. 
Published: March 2023

Abstract
Purpose. Self-sampling is increasingly being used in screening programs, yet no studies to date have examined the impact of bodily characteristics on self-sampling experiences. Our objective was to assess whether body mass index (BMI) and physical disability were associated with anal self-sampling difficulty.
Methods.We recruited sexual minority men (SMM) and trans persons in Milwaukee, Wisconsin to participate in an anal cancer screening study. Between January 2020 and August 2022, 240 participants were randomized to a home (n=120) or clinic (n=120) screening arm. Home participants received a mailed at-home anal self-sampling kit and were asked to attend a baseline clinic visit where biometric measurements were collected. Participants were asked to complete a survey about their experience with the kit. This research utilizes data from participants who used the kit and completed a baseline clinic visit and post-swab survey (n=82). We assessed the impact of BMI and physical disability on reported body or swab positioning difficulty.
Results. Most participants reported no or little difficulty with body positioning (90.3%) or swab positioning (82.9%). Higher BMI was significantly associated with greater reported difficulty with body positioning (aOR=1.10, 95% CI 1.003-1.20, p=.04) and swab positioning (aOR=1.11, 95% CI 1.02-1.20, p=.01). Physical disability was not significantly associated with body or swab positioning difficulty. Specimen adequacy did not differ by BMI category (p=.76) or physical disability (p=.88).
Conclusion. Anal self-sampling may be a viable option to reach obese persons who may be more likely to avoid screening due to weight-related barriers.
Bivariate Sin-Generalized Exponential Model with Applications on Medicine, Economic, and Environmental Data
Authors: Hassanein W. A. , Sobhy A. M. 
Published: March 2023

Abstract
This paper focuses on answering the question; How we can construct a bivariate model with fewer parameters that serve many disciplines in lifetime data? The answer to this question is introduced in this paper by proposing a new bivariate model that depends on constructing a SinTransformation of its marginals in the Farlie-Gumbel Morgenstern (FGM) Copula Model. This bivariate model is named by Morgenstern Sin Generalized Exponential (MSGE) Distribution. Without adding any extra parameter, MSGE provides a statistical model which gives better results in the goodness of fit to medical, economic, and environmental real lifetime data compared with other well-known models. The mathematical procedure of MSGE is carried out for statistical and reliability properties, estimation via 3 methods, simulation, and real practical applications in the three mentioned disciplines. The results illuminate the effectiveness of MSGE for the practitioner without overloading extra parameters.
Exosome-derived circKIF20B suppresses gefitinib resistance and cell proliferation in non-small cell lung cancer
Authors: Wei Si-Liang , Ye Jing-Jing , Sun Li , Hu Lei , Wei Yuan-Yuan , Zhang Da-Wei , Xu Meng-Meng , Fei Guang-He 
Published: March 2023

Abstract
Background The gefitinib resistance mechanism in non-small cell lung cancer (NSCLC) remains unclear, albeit exosomal circular RNA (circRNA) is known to possiblyplay a vital role in it.
Methods We employed high-throughput sequencing techniques to detect the expressions of exosomalcircRNA both in gefitinib-resistant and -sensitive cells in this study. The circKIF20B expression was determined in serum exosomes and tissues of patients by qRT-PCR. The structure, stability, and intracellular localization of circKIF20B were verified by Sanger sequencing, Ribonuclease R (RNase R)/actinomycin D (ACTD)treatments, and Fluorescence in situ hybridization (FISH). The functions of circKIF20B were investigated by 5-Ethynyl-20-deoxyuridine (EdU), flow cytometry, Cell Counting Kit-8 (CCK-8), oxygen consumption rate (OCR), and xenograft model. Co-culture experiments were performed to explore the potential ability of exosomal circKIF20B in treatinggefitinib resistance. The downstream targets of circKIF20B were determined by luciferase assay, RNA pulldown, and RNA immunoprecipitation (RIP).
Results We found that circKIF20B was poorlyexpressed in the serum exosomes of gefitinib-resistant patients (n = 24) and the tumor tissues of patients with NSCLC (n = 85). CircKIF20B was negatively correlated with tumor size and tumor stage. Decreasing circKIF20B was found to promote gefitinib resistance by accelerating the cell cycle, inhibiting apoptosis, and enhancing mitochondrial oxidative phosphorylation (OXPHOS), whereas increasing circKIF20B was found to restore gefitinib sensitivity. Mechanistically, circKIF20B bound to miR-615-3p for regulating the MEF2A and then altering the cell cycle, apoptosis, and mitochondrial OXPHOS. Overexpressing circKIF20B parental cells can restore sensitivity to gefitinib in the recipient cells by upregulating the exosomal circKIF20B expression.
Conclusions This study revealed a novel mechanism of circKIF20B/miR-615-3p/MEF2A signaling axis involving progression of gefitinib resistance in NSCLC. Exosomal circKIF20B is expected to be an easily accessible and alternative liquid biopsy candidate and potential therapeutic target in gefitinib-resistant NSCLC.
Effects of fiber content and layer thickness on the final crack morphology of fine-grained coral soil by desiccation cracking tests
Authors: Ding Xuan-Ming , Fang Hua-Qiang , Ou Qiang , Jiang Chun-Yong , Li Yifu 
Published: March 2023

Abstract
Purpose
Fine-grained coral soil as a special kind of marine soil, its potential application value has become increasingly prominent with the strategic goal of 'building a maritime power' proposed. It is necessary to study the modification of fine-grained coral soil to improve its crack resistance, so as to be better applied to practical engineering.
Materials and methods
The effects of PVA fiber (polyvinyl alcohol fiber) content and layer thickness on the final crack morphology of fine-grained coral soil were studied. Through the desiccation crack test, the final crack parameters, clod parameters, fractal dimension and crack morphology distribution of fine-grained coral soil were obtained by using image processing technology, and the quantitative analysis was carried out. Finally, the final crack morphology of fine-grained coral soil under the coupling effect of fiber content and layer thickness was obtained.
Results and discussion
The increase of layer thickness can reduce the number of nodes and number of crack segments, the total crack length and the fractal dimension of crack length. However, it can lead to the increase of average crack width, average crack length, total crack area, surface crack rate and surface crack ratio. The relative frequency distribution ranges of crack length and crack width are also gradually expanding. Moreover, the frequency distribution of crack length and crack width of fine-grained coral soil conforms to the Lognormal function. When the fiber content is low or no fiber is added, the frequency distribution of clod area also conforms to the Lognormal function.
Conclusions
Our study suggested that the crack resistance of fine-grained coral soil is improved with the increase of fiber content, which effectively inhibits the extension of cracks along the length direction and the expansion of width direction. In addition, The layer thickness also has a significant effect on the soil desiccation cracks. Revealing its law is of great significance for the potential application of fine-grained coral soil.
Acupuncture treatment of essential hypertension
Authors: Hou Zhihui , Jia Yuqi , Zhang Xuecheng , Wang Qi , Dong Yujiang , Zhu Yongzheng , Fan Cuihong , Zhao Huanjun , Zhang Chunxiao , Zhang Yongchen , Jia Hongling 
Published: March 2023

Abstract
Objective: To study the effect of acupuncture on clinical symptom score, blood pressure (BP), ABI and baPWV index in patients with mild to moderate essential hypertension.
Methods: The single-blind Randomized controlled trial included 90 patients with mild to moderate essential hypertension who were randomly divided into two acupuncture groups and a control group. The control group was treated with Western medicine, while the experimental group was treated with medicine and acupuncture at different acupoints, Acupuncture Group Ⅰ and Acupuncture Group Ⅱ. The treatment lasted for 12 weeks and the indexes were collected before and after treatment. The primary outcome measure was changes in blood pressure. The secondary outcome measures were ABI, baPWV and TCM symptom score. The safety evaluation index was the incidence of adverse events.
Results: Acupuncture Group Ⅰ, Acupuncture Group Ⅱ and Control group all had 30 patients finished the study. The results showed that there were significant differences in blood pressure, ABI, baPWV index and TCM symptom score in the group compared with before intervention (P < 0.05). Acupuncture Group Ⅰ is superior to Acupuncture Group Ⅱ in improving systolic blood pressure, baPWV level, TCM symptom score and total effective rate. There are significant differences between the two groups and control group in various indexes, and the above differences are statistically significant (P<0.05). No significant complications occurred in the acupuncture group, no patient dropped out.
Conclusions: The results show that acupuncture can effectively reduce blood pressure, improve clinical symptoms, and improve the level of arteriosclerosis index with high safety. More effective acupuncture points have been explored.
Combining a noble gas with radiotherapy: Glutamate receptor antagonist xenon acts as a radiosensitizer in glioblastoma
Authors: Büttner Thomas , Maerevoet Marielena K. E. , Giordano Frank A. , Veldwijk Marlon R. , Herskind Carsten , Ruder Arne M. 
Published: March 2023

Abstract
Background
Ionotropic glutamate receptors AMPAR and NMDAR modulate proliferation, invasion and radioresistance in glioblastoma (GB). Pharmacological targeting is difficult as many in vitro-effective agents are not suitable for in patient applications. We aimed to develop a method to test the well tolerated AMPAR- and NMDAR-antagonist xenon gas as radiosensitizer in GB.
Methods
We designed a diffusion-based system to perform the colony formation assay (CFA), the radiobiological gold standard, under xenon exposure. Stable and reproducible gas atmosphere was validated with oxygen and carbon dioxide as tracer gases. After checking for AMPAR and NMDAR expression via immunofluorescence staining we performed the CFA with the glioblastoma cell lines U87 and U251 as well as the non-glioblastoma derived cell line HeLa. Xenon was applied after irradiation and additionally tested in combination with NMDAR antagonist memantine.
Results
The gas exposure system proved compatible with the CFA and resulted in a stable atmosphere of 50% xenon. Glutamate receptor subunits were present in glioblastoma-derived and to a lesser extent in HeLa cells. Significant radiosensitization by xenon was shown in U251 and U87 at irradiation doses of 4–8 Gy and 2–8 Gy, respectively (p < .05). The effect was further enhanced by the addition of memantine, showing significant reduction in the surviving fraction already at 2 Gy for both glioblastoma cell lines (p < .05). Xenon did not increase the radiosensitivity of HeLa cells until a radiation dose of 8 Gy.
Conclusion
The developed system allows for testing of gaseous agents with CFA. As a proof of concept, we have, for the first time, unveiled radiosensitizing properties of xenon gas in glioblastoma.
Single-Cell Sequencing Analysis Reveals the Correlation between Obesity and Circadian Rhythm in Spermatogenesis and Its Microenvironment
Authors: Wang Wei , Zhang Wei , Chen Jinshan , Wang Shuang , Mu Changjiang , Xi Yujia , Guo Qiang , Wang Jingqi 
Published: March 2023

Abstract
Background
Male infertility has gradually increased morbidity during these years. Due to the lack in understanding of specific mechanism of male infertility, the patients could not receive efficient treatment for a long period. Obesity was universally thought to correlate with male infertility. However, the underlying pathway of obesity inducing male infertility is still uncertain. Previous studies indicated that obesity could impact circadian rhythm in multiple tissues. Therefore, it's worth exploring the correlation between obesity and circadian rhythm and their effect to male infertility.
Methods
Based on a single-cell RNA sequencing dataset containing 12 human testicular tissues, we divided the testicular cells into different clusters. According to Body Mass Index (BMI), the cells were divided into normal (18.5 ≤ BMI ≤ 24.9), little fat (25.0 ≤ BMI ≤ 29.9) and very fat (30.0 ≤ BMI ≤ 34.9) groups. hdWGCNA was used to construct co-expression modules of circadian rhythm-related genes. CRD_scores were constructed to describe circadian rhythm conditions based on the modules. Differentially expressed genes (DEGs) between two groups were identified using the R package "seurat". Enrichment analysis were performed to explore the function of DEGs using relevant R packages. Statistical analysis including comparison and correlation analysis were also conducted using R.
Results
Co-expression modules were found to all correlate with BMI. Furthermore, circadian rhythm showed a significant correlation with obesity in every cell clusters. In spermatogenesis, obesity could induce circadian rhythm disruption (CRD) in related cells. In Leydig cells, obesity could disturb androgen production by inducing CRD, which will lead to androgen level disruption and further impact reproduction function. Two hub genes (STAT3 and CEBPD) in the red co-expression module were found to play an important part in the process of obesity inducing CRD in Leydig cells. In macrophages, relationship between obesity and circadian rhythm was found to correlate with inflammation response and spermatogenesis.
Conclusions
We determined the correlation between obesity and circadian rhythm in testicular cells. It's found that obesity-related CRD in testis could lead to disrupted spermatogenesis and androgen level, inflammation, which will cause male reproductive dysfunction and infertility.
Medical Sorting (TRIAGE): Implementation Problems and Legislative Ways to Overcome Them
Authors: Korobtsova Nataliia 
Published: March 2023

The relevance of the topic chosen for research is due to the situation in which Ukraine and the entire civilized world found themselves, – the war and the pandemic of the acute respiratory disease COVID-19, which is caused by the SARS-CoV-2 coronavirus. How it is possible to provide medical care to as many people as possible without violating the principles of reasonableness and fairness in its provision is a question that both medical professionals and legal scholars of all democratic countries of the world are trying to answer. And one of the ways to solve this problem is the use of medical triage (TRIAGE). The purpose of this article is to consider medical triage as one of the forced measures of providing medical aid in emergency conditions, when there is a lack of medicines, medical personnel, medical equipment, etc., with a large number of victims. With the help of an empirical research method, as well as methods of analysis, generalization and explanation, the author managed to consider the history and reasons for the emergence of medical triage, investigate and determine the prerequisites for its use, positive and negative features, the possibility and necessity of its use during pandemics and states of war. The author identified the problems that arise in the application of medical triage and proposed ways to overcome them, which is the result of this study.
Replacing the Persecution Condition for Refugeehood
Authors: Beaton Eilidh 
Published: March 2023

In this article, earlier versions of which were presented at the IVR World Congress in 2019 and in graduate student workshops at the University of Pennsylvania, the author offers a critical look at the existing definition of a refugee in international law, in particular, in the 1951 Convention relating to the Status of Refugees. The Convention recognizes a well-founded fear of persecution for reasons of race, religion, nationality, membership in a particular social group or political opinion as a condition for a person to be granted refugee status. However, this leaves significant gaps in the protection of persons fleeing indiscriminate or generalized violence or harm, although such people today constitute a significant proportion of those forced to move from their country of nationality. The author emphasizes that within some regions and countries there are legal instruments capable of bridging these gaps (the 1969 Organization of African Unity Convention on the Specific Aspects of Refugee Problems in Africa, the 1984 Cartagena Declaration on Refugees, as well as temporary protection in the EU and the US), but the UN Convention continues to be deficient in this regard.
The author proposes to correct these shortcomings by applying the “needs-first” approach, which understands refugees as all displaced persons fleeing sufficiently serious threats. The author presents her own definition of a refugee as “any person with a well-founded fear that their fundamental human rights are urgently threatened; who would have no recourse to their home government, even if appropriate international assistance were available; and whose interests can only or best satisfied by means of refugee – that is, by means of protection from a political authority that is not their own, usually in the form of asylum within the territory of that country.”
The author acknowledges that her proposed “needs-first” approach is not without its drawbacks, and the main challenge in its implementation is to avoid becoming a purely humanitarian measure and to preserve the political identity of the individuals who are fleeing the threat to countries not of their nationality.
Suitability of drinking water quality in Chittagong Metropolitan City, Bangladesh: research on urban water bodies (UWBs) using multivariate analytic techniques
Authors: Molla Morshed Hossan , Chowdhury Mohammad Abu Taiyeb , Muhibbullah Md. , Ali Kazi Md. Barkat , Bhuiyan Md. Habibur Rahman , Das Suman , Morshed A.J.M. , Das Jewel , Islam Saiful 
Published: March 2023

AbstractThis is empirical research that focuses on the urban water ecosystems in the humid tropics of South Asia. The purpose of the study was to evaluate the quality of drinking water in the urban water bodies (UWBs) of Chittagong Metropolitan City (CMC), Bangladesh. The field data was centered on the analysis and depiction of twenty-three (23) water quality parameters, collected from twenty-one (21) spatial observation units. Analytic tools include suitability analysis, correlation matrix, principal component analysis (PCA), and cluster analysis (CA) as a means to an end. The data were analyzed using SPSS. The analysis reveals that drinking water quality in studied UWBs was inappropriate during the monsoon season. Parameters that crossed the extreme permissible concentration incorporate EC, BOD, COD, Turbidity, Nitrate, Total coliform, and Fecal coliform. The PCA extracted four factors (PC1–4) with an eigenvalue of 10.23, explaining 73.1% of the total variation in the dataset in cumulative terms. The CA recognized three (3) broad groups of the sampling stations. Group A represents nine cases, suffering the most from pollution concentration in CMC. Awareness building at all levels is advocated to improve clean water sources, increase service provision, and ensure public health safeguards.
A Review of Nanostructures in Electrowetting-on-dielectric Systems: From Nanostructured Dielectric Layers to Nanofluids
Authors: Budlayan Marco L. , Patricio Jonathan N. , Arco Susan D. , Guerrero Raphael A. 
Published: March 2023

Abstract:
The extensive interest in electrowetting-on-dielectric (EWOD) as a key in advancing
the efficiency and controllability of fluid-based microelectromechanical and actuator systems
has resulted in a deluge of technological research, especially in the area of microfluidics, liquid
lenses, and fluid-based lab-on-chips. More recently, the integration of nanostructures into
EWOD-driven devices has shown promising improvement in these devices’ performance, design,
and miniaturization. Due to the exceptional properties, availability, versatility, and tunability
of nanostructures, they are being utilized as components of EWOD systems for various applications.
Utilization ranges from fabricating nanodimensional dielectric layers to incorporating
nanoparticles in fluid droplets. With the current trend in improving the performance and functionality
of EWOD-driven devices at low voltage operations, it is timely to revisit the fundamental
principle of EWOD phenomena and how it is extended experimentally using nanostructures.
In this paper, we present the different nanostructures investigated as dielectric materials in various
EWOD experiments focusing on metal oxide and silicon nitride layers. Notes on the structure
of these dielectric layers are also presented. Furthermore, various EWOD experiments employing
nanofluid droplets are also described. This paper provides a clear picture of nanostructures’
diverse impact on the advancement of EWOD technology. The insights presented in this
paper may also serve as a guidepost for future exploration and development of the role of
nanostructures in EWOD-driven devices.