bluuebunny/crossref_metadata_2025_split huggingface dataset:
ONC201/TIC10 enhances durability of mTOR inhibitor everolimus in metastatic ER+ breast cancer
Authors: Farmaki Elena , Nath Aritro , Emond Rena , Karimi Kimya L , Grolmusz Vince K , Cosgrove Patrick A , Bild Andrea H 
Published: December 2022

SummaryThe mTOR inhibitor, everolimus, is an important clinical management component of metastatic ER+ breast cancer. However, most patients develop resistance and progress on therapy, highlighting the need to discover strategies that increase mTOR inhibitor effectiveness. We developed ER+ breast cancer cell lines, sensitive or resistant to everolimus, and discovered that combination treatment of ONC201/TIC10 with everolimus inhibited cell growth in 2D/3Din vitrostudies. We confirmed increased therapeutic response in primary patient cells progressing on everolimus, supporting clinical relevance. We show ONC201/TIC10, in metastatic ER+ breast cancer cells, mechanistically involves oxidative phosphorylation inhibition and stress response activation. Transcriptomic analysis in everolimus resistant breast patient tumors and mitochondrial functional assays in resistant cell lines demonstrated increased mitochondrial respiration dependency, contributing to ONC201/TIC10 sensitivity. We propose that ONC201/TIC10 and modulation of mitochondrial function may provide an effective add-on therapy strategy for patients with metastatic ER+ breast cancers resistant to mTOR inhibitors.
Spatial and functional arrangement of Ebola virus polymerase inside phase-separated viral factories
Authors: Fang Jingru , Castillon Guillaume , Phan Sebastien , McArdle Sara , Hariharan Chitra , Adams Aiyana , Ellisman Mark H. , Deniz Ashok A. , Saphire Erica Ollmann 
Published: December 2022

AbstractEbola virus (EBOV) infection induces the formation of membrane-less, cytoplasmic compartments termed viral factories, in which multiple viral proteins gather and coordinate viral transcription, replication and assembly. Key to viral factory function is the recruitment of EBOV polymerase, a multifunctional machine that mediates transcription and replication of the viral RNA genome. We show that intracellularly reconstituted EBOV viral factories are biomolecular condensates, with composition-dependent internal exchange dynamics that likely facilitates viral replication. Within the viral factory, we found the EBOV polymerase clusters into foci. The distance between these foci increases when viral replication is enabled. In addition to the typical droplet-like viral factories, we report the formation of network-like viral factories during EBOV infection. Unlike droplet-like viral factories, network-like factories are inactive for EBOV nucleocapsid assembly. This unique view of EBOV propagation suggests a form-to-function relationship that describes how physical properties and internal structures of biomolecular condensates influence viral biogenesis.
Development and evaluation of a formulation of probiontPhaeobacter inhibensS4 for the management of vibriosis in bivalve hatcheries
Authors: Takyi Evelyn , LaPorte Jason , Sohn Saebom , Stevick Rebecca J. , Witkop Erin M. , Gregg Lauren , Chesler-Poole Amanda , Small Jessica , White Meredith M. , Giray Cem , Rowley David C. , Nelson David R. , Gomez-Chiarri Marta 
Published: December 2022

AbstractLarval eastern oysters (Crassostrea virginica) grown in shellfish hatcheries are susceptible to bacterial diseases, particularly vibriosis. Probiotics are microbes that confer health benefits to the host and have been identified as promising tools to manage diseases in aquaculture. The marine bacteriumPhaeobacter inhibensS4 (S4) protects larval eastern oysters against challenge with the bacterial pathogenVibrio coralliilyticusRE22 (RE22). A concentrated liquid formulation of probiont S4 that maintained high cell viability after long-term storage was developed for commercial use in shellfish hatcheries. The safety and efficacy of the formulation was tested in six different trials in two hatcheries. The S4 formulation was added toC. virginicalarvae culture tanks daily at 104colony forming units (CFU)/mL from day 1 post fertilization until day 6, 12, or 14, depending on the trial. Treatment of larvae in the hatchery with the S4 formulation did not significantly affect the survival and growth of the larvae. Formulated probiont S4 treatment in the hatchery led to a significant increase in Relative Percent Survival (RPS) when larvae were subsequently challenged with the pathogen RE22 (105CFU/mL) for 24 hours in a laboratory challenge, as compared to probiotic-untreated RE22-challenged larvae (Relative Percent Survival increase of 46 - 74%,p< 0.05). These results suggest that this novel S4 formulation is a safe, easy to use, and effective tool in preventing larval losses due to vibriosis in hatcheries.
Scientific collaboration and thematic analysis of the tourism industry in the context of COVID-19: a bibliometric approach
Authors: Yang Luqi , Li Xiaoni , Hernández-Lara Ana-Beatriz 
Published: December 2022

Purpose
The main purpose of this research paper is to generate a holistic bibliometric study of the tourism industry and COVID-19 fields, to further investigate the current interests and trends emerging from scientific collaboration and thematic analysis and to identify research gaps that indicate future research directions.

Design/methodology/approach
This study conducts several analyses, which include the co-authorship and social network analysis, co-citation and keyword co-occurrence knowledge structures. The authors generate a knowledge map of the leading articles and link them with previous literature to elucidate the debates and consensus in research on COVID-19 and tourism.

Findings
Research interests concentrate in the USA, China, Europe and the Oceania areas, so more cross-continental collaborations are expected among them and with other regions. Popular topics are tourism sustainable transformation, crisis management and multidisciplinary fields like tourism, hospitality, information technology and environmental sciences. This paper also identifies underexplored topics for future investigation on the social, environmental, cultural and governance dimensions of sustainable tourism.

Research limitations/implications
This paper contributes to guiding tourism researchers in identifying and finding publication references and future collaborations. Moreover, the investigation of knowledge structures could be beneficial for scholars hoping to broaden the current understanding of this field and discover potential for future tourism research, especially in the global pandemic and other severe health crises.

Originality/value
This study enriches the existing literature in the fields of tourism and the pandemic and highlights current interests and research trends exploring scientific collaboration, thematic analysis and knowledge mapping.
Firm-specific political risk: a systematic investigation of its antecedents and implications for vertical integration and diversification strategies
Authors: Fan Di , Xiao Chengyong 
Published: December 2022

PurposeUncertainties caused by political risks can drastically affect global supply chains. However, the supply chain management literature has thus far developed rather limited knowledge on firms' perception of and reactions to increased political risks. This study has two main purposes: to explore the relationship between extant risk exposure and perceived firm-specific political risk and to understand the impact of firm-specific political risk on firms' vertical integration and diversification strategies.Design/methodology/approachThe authors developed a unique dataset for testing our hypotheses. Specifically, the authors sampled manufacturers (SIC20-39) listed in the United States from 2002 to 2019. The authors collected financial and diversification data from Compustat, vertical integration data from the Frésard-Hoberg-Phillips Vertical Relatedness Data Library and political risk data from the Economic Policy Uncertainty database. This data collection process yielded 1,287 firms (8,329 observations) with available data for analysis.FindingsA two-way fixed-effect regression analysis of panel data revealed that firms tend to be more sensitive to political risk when faced with income stream uncertainty or strategic risk. By contrast, exposure to stock returns uncertainty does not significantly influence firms' sensitivity toward political risk. Moreover, firm-specific political risk is positively associated with vertical integration and product diversification. However, firm-specific political risk does not result in higher levels of geographical diversification.Originality/valueThis study joins the literature that systematically explores the antecedents and implications of firm-specific political risk, thus broadening the scope of supply chain risk management.
Assessing retailer readiness to use blockchain technology to improve supply chain performance
Authors: Behl Abhishek , Jayawardena Nirma Sadamali , Pereira Vijay , Sampat Brinda 
Published: December 2022

PurposeThis paper aims to assess the readiness of retail workers to use blockchain technology (BCT) to improve supply chain performance. The assessment was made via a quantitative approach taken using a theoretical framework based on Keller’s motivation model and self-determination theory in the BCT context.Design/methodology/approachThe authors collected data from 567 retail workers from an emerging country through a structured survey questionnaire. The authors tested the hypotheses of the proposed model using Warp PLS 7.0 and controlled firm age, industry type and technological intensity.FindingsOur findings may help firms in making the process of digital transformation inclusive. The authors found that supplier-based attention and motivation through BCT lead to supply chain performance, and that supplier-based satisfaction and trust achieved through BCT positively impact supply chain performance. Further, supplier-based relevance on raw material selection with the higher trust and motivation levels achieved through BCT was found to have a positive impact on supply chain performance.Research limitations/implicationsIT supply chain applications are referred to as “lean” rather than “rich” because they still rely mainly on written and numerical means to present data. When the environment is less ambiguous, then less rich media can be used to facilitate communication. IT supply chain applications allow suppliers to spend time building relationships with other suppliers instead of focusing on administrative tasks, thus enhancing such relationships.Originality/valueThis study can be considered the first to assess retailer readiness to use BCT to improve supply chain performance through the theoretical lens of Keller’s motivation model and self-determination theory.
Teachers' pedagogical implementation of the National Large-Scale Assessment (NLSA): improving and advancing teaching-learning processes
Authors: Arviv Elyashiv Rinat , Avidov-Ungar Orit 
Published: December 2022

Purpose
Large-scale assessment has been used in many education systems as an instrument to evaluate educational performance nationally. This practice is based on the concept of epistemic governance which encourages school accountability. This study aims to explore teachers' perspectives regarding the value and uses of national large-scale assessment (NLSA), highlighting its relevance across contexts.

Design/methodology/approach
Using qualitative data, this paper presents the case of the Israeli NLSA tests – the Meitzav, while examining the perceptions and actions in which teachers engage to follow-up on the test results, and the extent to which they implement pedagogical change in light of the test results.

Findings
The findings showed that teachers tend to use the NLSA test results as a pedagogical tool to improve learning processes to a limited extent. They concede that most activity involving the tests at the school and class levels is dedicated to preparation and not to pedagogical change. Some explanations are suggested.

Originality/value
This paper discusses the theoretical and practical implications of the NLSA testing regime for the school, curriculum and pedagogy.
FDI, digitalization and employment: empirical evidence from developing economies
Authors: Sinha Madhabendra , Tirtosuharto Darius , Ray Chaudhury Anjan , Basu Partha 
Published: December 2022

Purpose
The paper aims to empirically examine the impact of foreign direct investment (FDI) inflows and digitalization on employment opportunities in selected 70 developing economies across the world over the period of 2001–2019. The same empirical investigations are also carried out on two groups of these developing countries created on the basis of the levels of FDI inflows and digitalization.

Design/methodology/approach
The study uses various panel unit root tests followed by the estimations of the generalized method of moments in the dynamic panel framework, using secondary data collected from the World Bank (2020), International Labour Organization (2020) and International Telecommunication Union (2020).

Findings
Empirical findings reveal that both FDI inflows and digitalization have positive effects on employment; however, the extent of the impact of digitalization is greater than that of FDI inflows in developing economies, mostly in countries with relatively low FDI inflows and low digitalization.

Originality/value
Conventionally, FDI inflows accelerate economic growth and thus improve the labour market in host countries. However, FDI inflows into developing countries with low-skilled labours may limit job creation, particularly during the process of digitalization. This study shows that despite a much moderate impact of FDI inflows, digital transformation supports a higher employment in developing economies with low level of FDI inflows and digitalization.
The resentful undergrowth of nostalgia: Ontological insecurity, relative deprivation and powerlessness
Authors: Rogenhofer Julius Maximilian , Abts Koen , Klein Oliver , Bertin Paul 
Published: December 2022

AbstractThis article untangles competing conceptualisations of nostalgia and identifies a specific form of collective‐restorative nostalgia as politically significant. We argue that the link between resentment and this type of nostalgia emerges from their joint critique of the socio‐political realities of the present. Nostalgia provides spatial and temporal orientations for a group's experiences of resentment through highly selective recollections of the heartland and an idealised golden age. We hypothesize that nostalgia leverages the heartland and the golden age to formulate claims for recognition and restored status on behalf of those who feel left behind by late modernity. Next, the article uses structural equation modelling and the 2019 Belgian National Election Study to reveal how resentment (consisting of ontological insecurity, group relative deprivation, and powerlessness) mediates between structural characteristics and nostalgia. Our findings suggest that each component of resentment individually contributes to explaining the nostalgia of less educated and economically deprived individuals.
Infant feeding in an emergency: An oversight in United Kingdom emergency planning
Authors: Wise Patricia A. 
Published: December 2022

AbstractFollowing the finding of the World Breastfeeding Trends Initiative 2016 assessment that infant feeding is not mentioned in United Kingdom emergency planning guidance, in 2017 information was sought about Local Resilience Forum (LRF) planning. This research covered both guidance for parents on LRF websites and local planning. Considerable variation between areas and a dependence on the existing knowledge and experience of frontline staff was found. In 2020−2022 LRF websites in England and Wales were revisited and information requested about rest centre facilities, procurement of items needed by evacuated parents, provision of skilled support and staff training. Twenty‐nine of the 42 LRFs replied, partially or fully, directly or through their local authorities. The variation between areas found in 2017 was confirmed. Areas endeavour to provide for the needs of families but specific training in infant feeding was lacking, although several requested relevant resources or responded that the lack of training would now be reviewed. A short leaflet was produced and circulated to LRFs that had responded to aid training. Using established international guidance to include infant feeding in United Kingdom emergency planning guidance, implemented by national coordinators, would improve consistency between LRFs and better protect infant, child and maternal health and well‐being.
Market potential and value‐added opportunities of cold‐hardy berries and small fruits in the Intermountain West, USA
Authors: Garg Sumedha , Leisso Rachel , Kim Sun‐Hwa , Mayhew Emily , Song Mei , Jarrett Bridgid , Kuo Wan‐Yuan 
Published: December 2022

AbstractNovel cold‐hardy berries and small fruits represent an opportunity for growers in the Intermountain West, USA, as the harsh environment is not suited for other common berries and small fruits. This study analyzed the fresh market and value‐added potential of haskap berries (Lonicera caerulea), saskatoon berries (Amelenchier alnifolia), and dwarf sour cherry (DSC) fruit (Prunus x kerrasis) by instrumental and consumer studies. Fresh and 2‐week stored haskap (cv. Aurora), saskatoon (Lee 3), and DSC (Romeo) were measured for fruit weight, flesh firmness, bulk titratable acidity, pH, and soluble solid content. Participants (n = 115) in at‐home sensory tests scored these fruits for overall liking (OL, 9‐point hedonic scale), purchase intent (PI, 5‐point scale), and willingness‐to‐pay (WTP, 5‐point scale). Ten participants further shared insight on these fruits in focus groups. Instrumental testing found a significant decrease in flesh firmness for 2‐week stored haskap, but the consumers’ OL was still comparable to fresh haskap. The fresh and 2‐week stored haskap received significantly higher OL, PI, and WTP scores (7.7 ± 1.0, 3.8 ± 1.0, and $3.7 ± 1.0; 7.7 ± 1.2, 3.8 ± 1.1, and $3.7 ± 1.0, respectively) compared to saskatoon (6.1 ± 1.8, 2.8 ± 1.1, and $3.0 ± 0.9, respectively) and DSC (5.6 ± 2.2, 2.5 ± 1.2, and $3.1 ± 1.0, respectively) (α = 0.05). The focus groups indicated that participants want to support local produce. The participants expressed interest in fresh unprocessed haskap berries, but preferred saskatoon and DSC in different value‐added formats. Hence, this study concluded that there is an untapped fresh‐market potential for haskap berries and there are value‐added opportunities to extend the sale season and improve the palatability of saskatoon and DSC.Practical ApplicationThis research has examined consumer perception of three species of novel small fruits by determining fresh‐market potential and linking this to the opportunity for value‐added product development. For haskap berries, the study not only indicated fresh market potential, but for the cultivar Aurora, consumer liking was not meaningfully altered by 2 weeks of cold storage. These results are meaningful because they will assist growers in the Intermountain West with market planning, including the possibility of formulating products that utilize these novel crops. This study provides growers the opportunity to diversify their income stream by utilizing local produce.
Biocatalyst collection and heterologous expression of sesquiterpene synthases from basidiomycetous fungi: Discovery of a novel sesquiterpene hydrocarbon
Authors: Masunaga Natsuki , Kitaoka Takuya , Ichinose Hirofumi 
Published: December 2022

AbstractBasidiomycetes produce a wide variety of sesquiterpenoids, which attract significant interest in pharmaceutical and industrial applications. Structural diversification of sesquiterpenoids is performed by sesquiterpene synthases (STSs), which produce a wide array of backbone structures; therefore, functional characterization and increased biocatalyst collection of STSs are important for expanding scientific knowledge and meeting the needs of advanced biotechnology. Gene identification and functional annotation of STSs from the basidiomycetous fungi Agaricus bisporus, Auriscalpium vulgare, Lepista nuda, Pleurotus ostreatus and Trametes versicolor were conducted. Through these investigations, the catalytic functions of 30 STSs were revealed using recombinant enzymes heterologously expressed in Saccharomyces cerevisiae. Furthermore, the unique function of an STS from P. ostreatus, PoSTS‐06, was revealed to be the production of a novel sesquiterpene hydrocarbon that we named pleostene. The absolute structure of pleostene was determined by NMR spectroscopy and X‐ray crystallography using the crystalline sponge method.
High diagnostic yield of targeted next‐generation sequencing panel as a first‐tier molecular test for the patients with myopathy or muscular dystrophy
Authors: Çavdarlı Büşranur , Köken Özlem Yayici , Satılmış Saide Betül Arslan , Bilen Şule , Ardıçlı Didem , Ceylan Ahmet Cevdet , Gündüz Cavidan Nur Semerci , Topaloğlu Haluk 
Published: December 2022

AbstractMuscular dystrophies are a heterogeneous group of neuromuscular disorders with a wide range of the clinical and genetic spectrum. Whole‐exome sequencing (WES) has been on the rise to become the usual method of choice for molecular diagnosis in patients presenting with muscular dystrophy or congenital or metabolic myopathy phenotype. Here, we used a panel with 47 genes including not only muscular dystrophy but also myopathy‐associated genes that had been used as a first‐tier approach. A total of 146 patients who were referred to our clinic with the prediagnosis of muscular dystrophy and/or myopathy were included in the study. Dystrophin gene deletion/duplication was ruled out on the patients with a preliminary diagnosis of Duchenne muscular dystrophy. In this study, the molecular etiology of 67 patients was proved with the gene panel with a diagnostic yield of 46%. Causal variants were identified in 23 genes including CAPN3(11), DYSF(9), DMD(8), SGCA(5), TTN(4), LAMA2(3), LMNA(3), SGCB(3), COL6A1(3), DES (2), CAV3(2), FKRP(2), FKTN(2), ANO5, COL6A2, CLCN1, GNE, POMGNT1, POMGNT2, POMT2, SYNE1, TCAP, and FLNC with 16 novel variants. There were 27 patients with uncertain molecular results including the ones who had a variant of uncertain significance, who had only one heterozygous variant for an autosomal recessive disease, and the ones who had two variants in different genes. Molecular diagnosis in muscular dystrophy is essential to plan clinical management and choosing treatment options. Also, the results will affect the reproduction options. Targeted next‐generation sequencing is a cost‐effective method that reduces the WES requirements with a significant diagnostic rate.
The land use consequences of rural to urban migration*
Authors: Brewer Julia , Larsen Ashley , Noack Frederik 
Published: December 2022

AbstractRural to urban migration (RUM) is a key component of economic development, but its environmental consequences are not well understood. Here, we study the impacts of RUM on agriculture and land use using household panel data in combination with tree cover data from Uganda. Our results show that the labor loss and the inflow of remittances from RUM lead to a reduction in crop diversity but no shift toward less labor‐intensive crops or crops with a high up‐front investment. In addition to those results at the intensive margin, we find a reduction of cultivated area at the household level, which translates into reduced tree cover loss at the district level. These results suggest an important but nuanced role of RUM for land use change.