bluuebunny/crossref_metadata_2025_split huggingface dataset:
Identification of diverse antibiotic resistant bacteria in agricultural soil with H218O stable isotope probing combined with high-throughput sequencing
Authors: Hernández Marcela , Roy Shamik , Keevil C. William , Dumont Marc G. 
Published: April 2023

Abstract
Background
We aimed to identify bacteria able to grow in the presence of several antibiotics including the ultra-broad-spectrum antibiotic meropenem in a British agricultural soil by combining DNA stable isotope probing (SIP) with high throughput sequencing. Soil was incubated with cefotaxime, meropenem, ciprofloxacin and trimethoprim in 18O-water. Metagenomes and the V4 region of the 16S rRNA gene from the labelled “heavy” and the unlabelled “light” SIP fractions were sequenced.

Results
An increase of the 16S rRNA copy numbers in the “heavy” fractions of the treatments with 18O-water compared with their controls was detected. The treatments resulted in differences in the community composition of bacteria. Members of the phyla Acidobacteriota (formally Acidobacteria) were highly abundant after two days of incubation with antibiotics. Pseudomonadota (formally Proteobacteria) including Stenotrophomonas were prominent after four days of incubation. Furthermore, a metagenome-assembled genome (MAG-1) from the genus Stenotrophomonas (90.7% complete) was retrieved from the heavy fraction. Finally, 11 antimicrobial resistance genes (ARGs) were identified in the unbinned-assembled heavy fractions, and 10 ARGs were identified in MAG-1. In comparison, only two ARGs from the unbinned-assembled light fractions were identified.

Conclusions
The results indicate that both non-pathogenic soil-dwelling bacteria as well as potential clinical pathogens are present in this agricultural soil and several ARGs were identified from the labelled communities, but it is still unclear if horizontal gene transfer between these groups can occur.
Electrically assisted cycling for individuals with type 2 diabetes mellitus: a pilot randomized controlled trial
Authors: Bourne Jessica E. , Leary Sam , Page Angie , Searle Aidan , England Clare , Thompson Dylan , Andrews Robert C. , Foster Charlie , Cooper Ashley R. 
Published: April 2023

Abstract
Background
Type 2 diabetes mellitus (T2DM) and its associated complications puts considerable strain on healthcare systems. With the global incidence of T2DM increasing, effective disease management is essential. Physical activity (PA) is a key component of T2DM management; however, rates of PA engagement are low in this population. Developing effective and sustainable interventions that encourage PA is a high priority. Electrically assisted bicycles are becoming increasingly popular and may increase PA in healthy adults. This study aimed to provide evidence of the feasibility of conducting a randomized controlled trial to evaluate the efficacy of an e-cycling intervention to increase PA and improve health in individuals with T2DM.

Methods
A parallel-group two-arm randomized, waitlist-controlled pilot study was conducted. Individuals were randomized to either an e-bike intervention or standard care. The intervention incorporated two one-to-one e-bike skills training and behavioural counselling sessions delivered by a community-based cycling charity, followed by a 12-week e-bike loan with two further sessions with the instructors. Feasibility was assessed via measures related to recruitment, retention and intervention implementation. Post-intervention interviews with instructors and participants explored the acceptability of the study procedures and intervention. Clinical, physiological and behavioural outcomes were collected at baseline and post-intervention to evaluate the intervention’s potential.

Results
Forty participants (Mage = 57) were randomized, of which 34 were recruited from primary care practices. Thirty-five participants were retained in the trial. The intervention was conducted with high fidelity (> 80% content delivered). E-bike training provided participants with the skills, knowledge and confidence needed to e-bike independently. Instructors reported being more confident delivering the skills training than behavioural counselling, despite acknowledging its importance. The study procedures were found to be acceptable to participants. Between-group differences in change during the intervention were indicative of the interventions potential for improving glucose control, health-related quality of life and cardiorespiratory fitness. Increases in overall device measured moderate-to-vigorous PA behaviour following the intervention were found, and there was evidence that this population self-selected to e-cycle at a moderate intensity.

Conclusions
The study’s recruitment, retention, acceptability and potential efficacy support the development of a definitive trial subject to identified refinements.

Trial registration
ISRCTN, ISRCTN67421464. Registered 17/12/2018.
Instead of ‘writing against’ and discarding ‘immigrants’ integration, why not reconceptualize integration as a wicked concept?
Authors: Kutor Senanu Kwasi , Arku Godwin , Bandauko Elmond 
Published: April 2023

AbstractOver the years, some scholars have not only written against the concept of immigrant integration but have called for its rejection and abandonment. Critics argue that the concept is normative, objectifies others, mirrors outmoded imaginary of society, orients towards methodological nationalism, and a narrow emphasis on immigrants in the forces defining integration progression. Nonetheless, the concept continues to receive academic and policy attention. Against the backdrop of this polarized view, this paper raises an important question relating to the benefit or otherwise of writing against the concept of integration in the field of integration studies. Specifically, the paper asks: Is it appropriate to write against and reject the concept of integration? The paper responds to this question from a provocative conceptual perspective. Here, the paper argues that when the concept is purged of its inherent criticisms and rather reconceptualize as a wicked concept, it still offers a unique analytical spectrum with which scholars can approach several substantive critical questions regarding immigrants’ integration.
Relationship between 25-hydroxyvitamin D and IGF1: a cross-sectional study of the Third National Health and Nutrition Examination Survey participants
Authors: Li Wei , Yu Tao 
Published: April 2023

Abstract
Background
25-Hydroxyvitamin D (25OHD) and insulin-like growth factor 1 (IGF1) are crucial for bone health. Some studies have shown that they interact, whereas others have indicated no association. However, it remains inconclusive whether the interaction between the two is dose dependent. Herein, we explored the relationship between 25OHD and IGF1 by conducting a cross-sectional study.

Methods
This study involved 6,046 individuals from the Third National Health and Nutrition Examination Survey (NHANES III). The dependent and independent variables were IGF1 and 25OHD levels, respectively. The covariates included age, sex, race, BMI, exercise, smoking behavior, alcohol intake, diabetes, and serum calcium level. Multiple linear regression and generalized additive model were employed to analyze the relationship between 25OHD and IGF1. Interaction and hierarchical analyses were also performed.

Results
The 25OHD and IGF1 levels positively correlated after adjusting for covariates (β = 0.16, 95% CI: 0.04–0.29, P = 0.0103). Smooth curve fitting demonstrated a curvilinear relationship. When the 25OHD level was < 75 nmol/L, a positive correlation (β = 0.43, 95% CI: 0.25–0.62, P < 0.0001) was observed. When the 25OHD level was > 75 nmol/L, a negative correlation was observed (β = −0.53, 95% CI: −0.90 to −0.15, P = 0.0057).

Conclusion
This study demonstrated a nonlinear relationship between 25OHD and IGF1. It suggests that keeping the 25OHD level within a specific range may be more conducive to bone health. Additionally, when IGF1 is used to evaluate the efficacy and safety of recombinant human growth hormone (rhGH) in growth hormone deficiency treatment, the effect of 25OHD on the actual IGF1 level should be taken into account.
Case of cerebrotendinous xanthomatosis with giant xanthomas and literature review
Authors: Eliaçık Sinan , Çil Gülsüm 
Published: April 2023

Abstract
Introduction
Cerebrotendinous xanthomatosis (CTX) is a rare autosomal
recessive disease that occurs as result of mutation in the CYP27A1 gene. The
clinical presentation of the disease is quite wide. We planned to briefly
review the literature with this case diagnosed as a CTX.

Case
A 50-year-old male patient was admitted to the neurology
outpatient clinic complaining of progressive worsening of his walking, and
swelling in his legs. Mild mental retardation was detected in our patient. In
addition to the visual impairment that would be explained by cataracts, he had
xanthomas in both lower extremities. Signs related to bilateral cataract
surgery and intraocular lens were detected during an eye examination. There
were no abnormal findings in electroencephalography, electroneuromyography, and
brain magnetic resonance imaging of the patient, whom we learned that her
visual impairment started in childhood. The Mignarri Suscipion Index index was
calculated as 275. A genetic examination was requested and the CYP27A1 gene was
p.A216P (c.The mutation 646G>C) (CM044609) was detected as homozygous.

Conclusion
Due to the low
awareness of CTX and the variability of its clinical findings, its diagnosis may
be delayed for years, as in our patient. When diagnosed, most patients may have
severe, often irreversible neurological damage. With the early recognition of
the CTX and the start of treatment, patients can have a chance to quality life.
Pathogenic function of the natural variation of CP in WYMV and CWMV
Authors: Lei Jiajia , Liu Shuang , Ye Zhuangxin , Chen Zhiqing , Liu Hanhong , Zhong Kaili , Lu Qisen , Zhang Juan , Chen Jianping , Yang Jian , Liu Peng 
Published: April 2023

AbstractThe soil-borne viral disease, caused by wheat yellow mosaic virus (WYMV) and Chinese wheat mosaic virus (CWMV), is one of the most destructive wheat diseases in China. Considering the large wheat growing area in China, the genetic diversity of WYMV and CWMV could be high in the country. However, studies on genetic diversity of WYMV and CWMV in China are limited, making it difficult to prevent and control viral diseases on wheat. During 2021–2022, the wheat leaves with typical yellow mosaic virus symptoms were randomly collected from wheat fields in seven provinces. Nine WYMV and one CWMV isolates were identified in the samples using small RNA sequencing and RACE technology. Sequence alignment showed that several amino acid substitutions were occurred in the coat protein (CP) from these isolates. Moreover, we replaced the CP of WYMV and CWMV infectious clones with CP of newly identified isolates and found that natural variation of CP is involved in the pathogenicity of WYMV. Moreover, the WYMV infectious clones containing CP of the WYMV isolates from Junan at Shandong Province or Yangzhou at Jiangsu Province have enhanced WYMV infection in several local wheat resistance cultivars. Taken together, our findings suggest that the distribution of WYMV and CWMV in wheat growing areas has expanded in these years and the natural variation of viral genome is involved in pathogenicity of WYMV. Our results also provide a theoretical basis to explain the real distribution of wheat viral resistance varieties in China.
From face detection to emotion recognition on the framework of Raspberry pi and galvanic skin response sensor for visual and physiological biosignals
Authors: Patil Varsha Kiran , Pawar Vijaya R. , Randive Shreiya , Bankar Rutika Rajesh , Yende Dhanashree , Patil Aditya Kiran 
Published: April 2023

AbstractThe facial and physiological sensor-based emotion recognition methods are two popular methods of emotion recognition. The proposed research is the first of its kind in real-time emotion recognition that combines skin conductance signals with the visual-based facial emotion recognition (FER) method on a Raspberry Pi. This research includes stepwise documentation of method for automatic real-time face detection and FER on portable hardware. Further, the proposed work comprises experimentation related to video induction and habituation methods with FER and the galvanic skin response (GSR) method. The GSR data are recorded as skin conductance and represent the subject's behavioral changes in the form of emotional arousal and face emotion recognition on the portable device. The article provides a stepwise implementation of the following methods: (a) the skin conductance representation from the GSR sensor for arousal; (b) gathering visual inputs for identifying the human face; (c) FER from the camera module; and (d) experimentation on the proposed framework. The key feature of this article is the comprehensive documentation of stepwise implementation and experimentation, including video induction and habituation experimentation. An illuminating aspect of the proposed method is the survey of GSR trademarks and the conduct of psychological experiments. This study is useful for emotional computing systems and potential applications like lie detectors and human–machine interfaces, devices for gathering user experience input, identifying intruders, and providing portable and scalable devices for experimentation. We termed our approaches "sensovisual" (sensors + visual) and "Emosense" (emotion sensing).
Spontaneous urinary bladder perforation presenting as bowel obstruction in a child: a case report
Authors: AlAbadla Abdallah , AlOmari Najeh , Al-Halaseh Salameh , Al Hanaqtah Dua’a , AlBalwa Mohammad 
Published: April 2023

Abstract
Background
Spontaneous perforation of the urinary bladder is a rare disease entity. Due to its rarity, it is often hard to diagnose.

Case presentation
We present a case of a 2-year-old patient who presented with bowel obstruction and renal failure after scheduled correctional vertebroplasty due to congenital kyphosis. During exploratory laparotomy, two perforations were found at the dome of the urinary bladder, and an idiopathic spontaneous perforation of the urinary bladder was diagnosed. Primary double repair of the perforations was done, and no primary or secondary cause of the perforation was found.

Conclusion
The constellation of an acute abdomen and abnormal kidney function tests should increase the index of suspicion towards urinary bladder perforation. Due to its rarity, exploratory laparotomy is the gold standard method of diagnosis and treatment.
Development of a machine-learning-based method for early fault detection in photovoltaic systems
Authors: Voutsinas Stylianos , Karolidis Dimitrios , Voyiatzis Ioannis , Samarakou Maria 
Published: April 2023

AbstractIn the process of the decarbonization of energy production, the use of photovoltaic systems (PVS) is an increasing trend. In order to optimize the power generation, the fault detection and identification in PVS is significant. The purpose of this work is the study and implementation of such an algorithm, for the detection as many as faults arising on the DC side of a photovoltaic system. A machine learning technique was chosen. The dataset used to train the algorithm was based on a year’s worth of irradiance and temperature data, as well as data from the PV cell used. The method uses logistic regression with cross validation as a new approach to detect and identify faults in PVS. It is applied to smart PV arrays, that can transmit voltage and current measurements from each PV cell of the array individually. The results are satisfactory since the algorithm can detect the majority of faults that occur on the DC side of a photovoltaic (open-circuit fault, short-circuit fault, mismatch faults). The accuracy of the algorithm (97.11%) is comparable to other methods presented by the literature. Moreover, the computational cost of the proposed method is significantly lower than the methods presented in the literature. In summary, the performance of the implemented algorithm is considered particularly satisfactory and can be easily applied to PVS.
Repeat aortic valve replacement (AVR) for pseudoaneurysm of mitral-aortic intervalvular fibrosa (P-MAIVF) repair due to hemolytic anemia 6 years after AVR: a case report
Authors: Yamauchi Masanobu , Kanetsuki Kazuma , Hanada Tomoki , Kamihira Satoshi 
Published: April 2023

Abstract
Background
Pseudoaneurysm of the mitral-aortic intervalvular fibrosa (P-MAIVF) after infective endocarditis and/or valve replacement is rarely reported, and transesophageal echocardiography and cardiac multidetector computed tomography are useful for diagnosis. Surgery is mostly recommended to prevent fatal complications.

Case presentation
A 61-year-old man underwent repeat aortic valve replacement (AVR) with repair of a P-MAIVF due to hemolytic anemia 6 years after AVR, and 4 months after treatment of sepsis and an infected abdominal aortic aneurysm. Two years after the surgery, the patient is alive and well with no recurrence.

Conclusions
The present case was considered to be a very rare case in which surgery was performed because the blood flow entering and leaving the P-MAIVF contacted the prosthetic valve ring, resulting in hemolysis, severe anemia, and heart failure.
Strong evidence of inter-layer optical phonons and low-temperature acoustic-phonon bunching in WS2/WSe2 hetero-structure
Authors: Sarmah Hemanga J. , Mohanta D. 
Published: April 2023

Abstract
The nature of inter-layer optical phonons observed in the

hetero-structure is being reported in this work. Additionally, phonon bunching between

modes of both

and

can be ascertained in the acoustic region. The second-order bunching factor,

, is seen to improve sharply upon reduction of temperature, below a critical temperature. Moreover, the integral intensity of the peaks increases almost exponentially with the applied power of the laser source. To a good approximation, the phonon frequency corresponding to most of the Raman active modes varies linearly with altered temperature (78–300 K). However, the

mixed mode located at

exhibits a non-linear temperature dependence over the working range of temperature. Prevailing phonon bunching aspects of the phonon replica discussed in terms of bunching factor, frequency correlation parameter, etc., are highlighted based on acquired low-temperature Raman data and theoretical analogy.
Gravitational lensing by Morris-Thorne–type wormhole space-time with cosmic strings
Authors: Ahmed Faizuddin 
Published: April 2023

Abstract
In this paper, we study the deflection angle of photon light in Morris-Thorne–type wormhole space-time with a cosmic string. It is shown that the angle of deflection of photon light as well as the effective potential (time-like or light-like geodesics) of the system is influenced by the cosmic string and is higher compared to the known result.
Superconductivity in the thorium nickel borocarbide ThNiBC
Authors: Li Bai-Zhuo , Ji Liang-Wen , Li Miao-Cong , Song Shi-Jie , Wu Si-Qi , Zhu Qin-Qing , Ren Zhi , Xu Zhu-An , Cao Guang-Han 
Published: April 2023

Abstract
We report on the synthesis, crystal structure, physical properties, and first-principles calculations of a new borocarbide ThNiBC. The new compound was synthesized by an arc-melting method, crystallizing in a tetragonal LuNiBC-type structure with an exceptionally low axial ratio of

. The physical property measurements indicate that it is a Pauli-paramagnetic metal with significant electron-electron scattering. Bulk superconductivity at T
c = 0.7 K is demonstrated with zero electrical resistivity, Meissner effect, and specific-heat jump. The first-principles calculations reveal a relatively low density of states at the Fermi level with multiple electron-type Fermi surfaces.
The Adverse Effects Profile of Teprotumumab
Authors: Stan Marius N , Krieger Christine C 
Published: April 2023

Abstract

Context
Teprotumumab therapy for thyroid eye disease (TED) patients represents a major step forward. It targets and inhibits the insulin-like growth factor-1 receptor (IGF-1R), and its effectiveness is based on its interconnectedness with the thyrotropin receptor. However, IGF-1R has a ubiquitous expression and several adverse effects have been reported with teprotumumab use.

Objective
Describing these adverse effects for better understanding is the purpose of this review.

Methods
We reviewed the oncological studies in which teprotumumab was initially used. Subsequently we reviewed the clinical trials for TED and then the case series and case reports associated with teprotumumab use since it is US Food and Drug Administration approval (January 2020). We focused on common and/or serious adverse effects reported with the use of teprotumumab.

Results
We described the common occurrence of hyperglycemia (10%-30% incidence), its risk factors and suggested management. Hearing changes are described, a broad spectrum from mild ear pressure to hearing loss (sensorineural mechanism). Risk factors, suggested monitoring, and possible upcoming therapies are reviewed. We also reviewed data on fatigue, muscle spasms, hair loss, weight loss, gastrointestinal disturbances, menstrual changes, and infusion reactions. We noted some discrepancies between adverse effects in oncological studies vs studies focused on TED, and we aimed to explain these differences.

Conclusion
The use of teprotumumab should consider patient's values and preferences in balancing the expected benefit with these potential risks. Future drugs targeting IGF-1R should investigate these adverse effects for a possible class effect. Combination therapies with different agents hopefully will be identified that maximize benefits and minimize risks.
Purification of human β- and γ-actin from budding yeast
Authors: Haarer Brian K. , Pimm Morgan L. , de Jong Ebbing P. , Amberg David C. , Henty-Ridilla Jessica L. 
Published: April 2023

ABSTRACT
Biochemical studies of human actin and its binding partners rely heavily on abundant and easily purified α-actin from skeletal muscle. Therefore, muscle actin has been used to evaluate and determine the activities of most actin regulatory proteins but there is an underlying concern that these proteins perform differently from actin present in non-muscle cells. To provide easily accessible and relatively abundant sources of human β- or γ-actin (i.e. cytoplasmic actins), we developed Saccharomyces cerevisiae strains that express each as their sole source of actin. Both β- or γ-actin purified in this system polymerize and interact with various binding partners, including profilin, mDia1 (formin), fascin and thymosin-β4 (Tβ4). Notably, Tβ4 and profilin bind to β- or γ-actin with higher affinity than to α-actin, emphasizing the value of testing actin ligands with specific actin isoforms. These reagents will make specific isoforms of actin more accessible for future studies on actin regulation.