bluuebunny/crossref_metadata_2025_split huggingface dataset:
Trapa natans L. Extract Attenuates Inflammation and Oxidative Damage in Cisplatin‐Induced Cardiotoxicity in Rats by Promoting M2 Macrophage Polarization
Authors: Matovic Vesna , Ljujic Biljana , Radojevic Ivana , Đelic Gorica , Kovacevic Marina Miletic , Zivanovic Suzana , Papic Milos , Milivojevic Nevena , Petrovic Ivica , Gazdic Jankovic Marina 
Published: January 2025

Background:Trapa natans L. fruits and leaf extracts have a broad range of immunomodulatory, anti‐inflammatory, and antioxidant effects; however, their effects on cardiac protection have not been investigated.Objective: The study aims to test the biological activity of Trapa natans L. extract (TNE) in cisplatin (CDDP)‐induced cardiotoxicity.Methods: Wistar albino rats received a single dose of CDDP intraperitoneally and TNE ones per day for 2 weeks orally. Cardiac inflammation, necrosis, and fibrosis were determined by histological and immunohistochemical analyses. Cytokines in rat sera and cardiac tissue were detected by enzyme‐linked immunosorbent assay (ELISA) and quantitative real‐time (qRT)‐PCR. Rat macrophages cultured in the presence of TNE for 48 h were harvested for flow cytometry, while supernatants were collected for cytokine and reactive oxygen species (ROS) measurement.Results: Application of TNE significantly attenuated CDDP induced cardiotoxicity as demonstrated by biochemical and histopathological analysis. Administration of TNE once daily for 14 days decreased level of proinflammatory (TNF‐α, IFN‐γ, and IL‐6) and prooxidative parameters (NO2, O2, and H2O2), while increased level of immunosuppressive IL‐10 and antioxidative glutathione (GSH), catalase (CAT) and uperoxide dismutase (SOD) in the systemic circulation. TNE treatment resulted in attenuated heart inflammation and fibrosis accompanied with the reduced infiltration of macrophages and reduced expression of proinflammatory and profibrotic genes in heart tissue of CDDP‐treated animals. In vitro lipopolysaccharide (LPS)‐stimulated macrophages cultured in the presence of TNE adopted immunosuppressive phenotype characterized by decreased production of proinflammatory cytokines and prooxidative mediators.Conclusion: Our study provides the evidence that TNE ameliorates cisplatin‐induced cardiotoxicity in rats by reducing inflammation and oxidative stress via promoting M2 macrophage polarization.
Using a Photovoice Intervention to Teach Nursing Students About Living Homeless
Authors: Sullivan Jessica , Henry Sheryl , Woith Wendy 
Published: January 2025

Background: Over 582,000 people live homeless in the United States daily. They have more health problems and are more likely to receive inadequate health care than people with adequate housing. They also report that nurses treat them uncivilly when they seek health care. Nurse educators teach students to care and advocate for those who are unhoused. Photovoice has been used as a strategy to teach nursing students, but we found no studies using this technique to teach about homelessness. The purpose of this study was to develop and test a photovoice intervention to teach nursing students what it is like to live with unstable living conditions.Method: The convenience sample for this mixed methods design consisted of 88 undergraduate nursing students in a health assessment course.Results: Scores for the intervention group improved, with a significant difference between groups in attitudes toward poverty. Students expressed a better understanding of the experience of living homeless, gained awareness of the challenges faced by those who live homeless, and stated this awareness will impact their future nursing practice. They further identified the importance of knowing what resources unhoused individuals may need to support their overall health and well‐being.Conclusion: Photovoice is an effective method to teach students about homelessness.
The Politics of Representation in New Saudi Arabian Cinema
Authors: Killick Anthony 
Published: January 2025

Abstract
Textual analyses of Saudi Arabian films serve to deepen our understanding of the shifting boundaries of permissibility that exist between artists and the state. Where new cultural industries have been developed as part of broader national efforts toward economic diversification, Saudi auteurs such as Meshal Al-Jaser have sought to use film to facilitate society-wide discussions on contentious issues such as war, women’s rights and religion. These priorities intersect at the point where social liberalization is conducive with job market expansion and the new globally projected image of moderate Saudi nationalism. Yet the tentative exploration of boundaries plays out in much finer detail on the screen, pointing to how new freedoms give rise to new forms of representation in the cinematic treatment of Saudi history and politics. Al-Jaser’s first feature film, Naga, defers polemical commentary on the capricious bond between state and religion through its use of symbolism and multi-genre aesthetics. As a text, it highlights the cultural interplay between filmmakers and the state, as well as the repertoire of tactics that artists may use to facilitate discussion and positive social changes.
Effect of Temporal Modulation of Tactile Stimulus on Time Perception
Authors: Takahashi Rei , Takayama Yasuko , Yotsumoto Yuko 
Published: January 2025

Abstract
Time perception varies across different sensory modalities, with previous research indicating that visual flicker can dilate perceived duration while auditory flutter can sometimes compress it. This study aimed to explore duration perception in the relatively understudied tactile modality and compare it with the visual modality. In Experiment 1A, we investigated the effect of temporal modulation on perceived duration using 1-s tactile flutter and visual flicker stimuli, either stationary or modulated at 6.5 Hz, 12.5 Hz, or 24.5 Hz. Participants reported which of two sequentially presented stimuli appeared longer in duration. Results demonstrated that both tactile flutter and visual flicker induced significant time distortions, with higher frequencies leading to greater duration dilation. Notably, the magnitudes of time distortion were significantly correlated between the two modalities. Experiment 1B assessed the roles of sensitivity and subjective saliency in these time distortions. Contrary to previous findings, saliency did not correlate with the observed time distortions in either modality, and sensitivity did not correlate with time distortions in the visual modality. These results suggest that, while tactile duration perception exhibits similarities to visual duration perception, the lack of correlation with saliency challenges the idea that time distortion is driven by subjective saliency.
Exploring the Affordances and Challenges of GenAI Feedback in L2 Writing Instruction: A Comparative Analysis With Peer Feedback
Authors: Mi (米裕丹) Yudan , Rong (荣觅) Mi , Chen (陈旭传) Xuchuan (Winnie) 
Published: January 2025

Purposes In light of the usefulness theoretical framework, this research seeks to explore the affordances and challenges of integrating a generative artificial intelligence (GenAI) tool named ERNIE Bot into the writing feedback process. Design/Approach/Method A qualitative design was adopted for this study. Twelve students were purposively chosen as the focal participants. Data came from multiple sources. Semi-structured interviews were used as the primary source, triangulated with other data sources including writing drafts, reviews from peers, and the chatlogs with ERNIE Bot. The data were inductively and deductively analyzed. Findings The study highlighted the technological, educational, and social benefits of GenAI feedback, including its timeliness and personalization (technological), its roles as both an essay editor and a responsive tutor (educational), and its ability to provide a supportive environment and an engaging process (social). Three categories of challenges were revealed, namely lack of AI literacy among students, potential risk of worsening writing ability, and inability to catch emotion and to be perceptive. Originality/Value Enlightened by the usefulness theoretical framework, the study discovered the technological, educational, and social affordances as well as the limitations of GenAI feedback, which provides insights for the application of GenAI in L2 writing instruction.
Scrotal exploration for suspected testicular torsion in adolescents and adults: analysis of a series of 135 cases
Authors: Kirakoya Brahima , Yameogo Clotaire , Kambou Morgane , Idogo Ouedan , Ayed Abdullah , Nacthagande Gille , Kabore Fasnewinde Aristide 
Published: January 2025

Abstract

Background
Testicular torsion (TT) is the abnormal twisting of the spermatic cord leading to strangulation of the testis. Any non-traumatic acute scrotal pain in adolescents and young adults is considered TT until proven otherwise. It is a urological emergency. The diagnosis is clinically based on symptoms and signs that raise a high index of suspicions. Scrotal exploration is necessary, which will provide diagnostic certainty and allow treatment.

Methods
This study aimed to assess the clinical predictors of TT. This was a retrospective cross-sectional study of patients aged 15 years and older who underwent surgical exploration for suspected TT from January 2005 to December 2021 at Teaching Hospital Yalgado Ouedraogo (Burkina Faso). We compared the demographic data and clinical findings of patients according to the intraoperative diagnosis.

Results
In total, 135 scrotal explorations were performed for suspected TT. The average age of the patients was 24.56 years. TT was found in 123 cases (91.11%), epididymo-orchitis in 8 cases (5.93%) and in 4 cases (2.96%) no abnormality was found. Scrotal swelling and elevation of the testicle were more common in TT than in other cases (p < 0.001 in both cases). Fever was more frequent when it was not a TT (p < 0.001). Scrotal Doppler ultrasound had a sensitivity of 91%. The orchiectomy rate was 60.16% (N = 74) in patients with TT.

Conclusion
The clinical findings based on scrotal pain and swelling, elevation of the testicle and the absence of fever are sufficient to suggest TT and to perform surgical exploration.
Genomic selection strategies to overcome genotype by environment interactions in biosecurity-based aquaculture breeding programs
Authors: Kang Ziyi , Kong Jie , Li Qi , Sui Juan , Dai Ping , Luo Kun , Meng Xianhong , Chen Baolong , Cao Jiawang , Tan Jian , Fu Qiang , Xing Qun , Luan Sheng 
Published: January 2025

Abstract

Background
Family-based selective breeding programs typically employ both between-family and within-family selection in aquaculture. However, these programs may exhibit a reduced genetic gain in the presence of a genotype by environment interactions (G × E) when employing biosecurity-based breeding schemes (BS), compared to non-biosecurity-based breeding schemes (NBS). Fortunately, genomic selection shows promise in improving genetic gain by taking within-family variance into account. Stochastic simulation was employed to evaluate genetic gain and G × E trends in BS for improving the body weight of L. vannamei, considering selective genotyping strategies for test group (TG) at a commercial farm environment (CE), the number individuals of the selection group (SG) genotyped at nucleus breeding center (NE), and varying levels of G × E.

Results
The loss of genetic gain in BS ranged from 9.4 to 38.9% in pedigree-based selection and was more pronounced when G × E was stronger, as quantified by a lower genetic correlation for body weight between NE and CE. Genomic selection, particularly with selective genotyping of TG individuals with extreme performance, effectively offset the loss of genetic gain. With a genetic correlation of 0.8, genotyping 20 SG individuals in each candidate family achieved 93.2% of the genetic gain observed for NBS. However, when the genetic correlation fell below 0.5, the number of genotyped SG individuals per family had to be increased to 50 or more. Genetic gain improved by on average 9.4% when the number of genotyped SG individuals rose from 20 to 50, but the increase in genetic gain averaged only 2.4% when expanding from 50 to 80 individuals genotyped. In addition, the genetic correlation decreased by on average 0.13 over 30 generations of selection when performing BS and the genetic correlation fluctuated across generations.

Conclusions
Genomic selection can effectively compensate for the loss of genetic gain in BS due to G × E. However, the number of genotyped SG individuals and the level of G × E significantly affected the extra genetic gain from genomic selection. A family-based BS selective breeding program should monitor the level of G × E and genotyping 50 SG individuals per candidate family to minimize the loss of genetic gain due to G × E, unless the level of G × E is confirmed to be low.
Epigenome-wide association study of objectively measured physical activity in peripheral blood leukocytes
Authors: Fragoso-Bargas Nicolas , Mcbride Nancy S. , Lee-Ødegård Sindre , Lawlor Deborah A. , Yousefi Paul D. , Moen Gunn-Helen , Opsahl Julia O. , Jenum Anne Karen , Franks Paul W. , Prasad Rashmi B. , Qvigstad Elisabeth , Birkeland Kåre I. , Richardsen Kåre R. , Sommer Christine 
Published: January 2025

Abstract

Background
Few studies have explored the association between DNA methylation and physical activity. The aim of this study was to evaluate the association of objectively measured hours of sedentary behavior (SB) and moderate physical activity (MPA) with DNA methylation. We further aimed to explore the association between SB or MPA related CpG sites and cardiometabolic traits, gene expression, and genetic variation.

Results
For discovery, we performed cross sectional analyses in pregnant women from the Epigenetics in pregnancy (EPIPREG) sample with both DNA methylation (Illumina MethylationEPIC BeadChip) and objectively measured physical activity data (SenseWear™ Pro 3 armband) (European = 244, South Asian = 109). For EWAS of SB and MPA, two main models were designed: model (1) a linear mixed model adjusted for age, smoking, blood cell composition, including ancestry as random intercept, and model (2) which was additionally adjusted for the total number of steps per day. In model 1, we did not identify any CpG sites associated with neither SB nor MPA. In model 2, SB was positively associated (false discovery rate, FDR < 0.05) with two CpG sites within the VSX1 gene. Both CpG sites were positively associated with BMI and were associated with several genetic variants in cis. MPA was associated with 122 significant CpG sites at FDR < 0.05 (model 2). We further analyzed the ten most statistically significant MPA related CpG sites and found that they presented opposite associations with sedentary behavior and BMI. We were not able to replicate the SB and MPA-related CpG sites in the Avon Longitudinal Study of Parents and Children (ALSPAC). ALSPAC had available objectively measured physical activity data from Actigraph (without steps/day available) and leucocyte DNA methylation data collected during adolescence (n = 408, European).

Conclusion
This study suggests associations of objectively measured SB and MPA with maternal DNA methylation in peripheral blood leukocytes, that needs to be confirmed in larger samples of similar study design.
ABO and RhD blood groups as contributors to dyslipidaemia – a cross-sectional study
Authors: Mickelsson Malin , Ekblom Kim , Stefansson Kristina , Liv Per , Själander Anders , Näslund Ulf , Hultdin Johan 
Published: January 2025

Abstract

Background
The ABO blood group system has shown an association with cardiovascular disease. The susceptibility to CVD is proposed to be partly mediated by dyslipidaemia in non-O individuals. Previous studies are scarce for the RhD blood group, but we recently showed that RhD − young individuals are associated with subclinical atherosclerosis. Hence, we sought to examine whether the ABO blood groups and RhD factor are associated with dyslipidaemia.

Methods
All participants were part of the VIPVIZA study, including 3532 individuals with available plasma lipid levels. Lipids were assessed as total, LDL, HDL, remnant, non-HDL cholesterol and triglycerides. Information about ABO and RhD was retrieved by linking VIPVIZA with the SCANDAT-3 database, where 85% of VIPVIZA participants were registered.

Results
For the ABO blood groups, no significant differences in lipid levels between non-O and O individuals were seen. In 40-year-old males, RhD − individuals compared to RhD + had higher levels of non-HDL cholesterol, LDL cholesterol, and remnant cholesterol, with ratios of geometric means of 1.21 (CI95% 1.03; 1.43), 1.20 (1.02; 1.41) and 1.38 (1.00; 1.92), respectively. No differences in lipid levels depending on the RhD blood group were seen in women or the older age groups.

Conclusion
Our study indicates that younger RhD − men have increased non-HDL, LDL, and remnant cholesterol levels. Thus, the RhD blood group, but not ABO, seems to be associated with dyslipidaemia and may act as a future possible risk marker of cardiovascular disease.

Graphical Abstract
Drug allergy
Authors: Jeimy Samira , Wong Tiffany , Ben-Shoshan Moshe , Copaescu Ana Maria , Isabwe Ghislaine A. C. , Ellis Anne K. 
Published: January 2025

Abstract
Drug allergy encompasses a spectrum of immunologically-mediated hypersensitivity reactions (HSRs) with varying mechanisms and clinical presentations. This type of adverse drug reaction (ADR) not only affects patient quality of life, but may also lead to delayed treatment, unnecessary investigations, and increased morbidity and mortality. Given the spectrum of symptoms associated with the condition, diagnosis can be challenging. Therefore, referral to an allergist experienced in the diagnosis and management of drug allergy is recommended if a drug-induced allergic reaction is suspected. Diagnosis relies on a careful history and physical examination and, in some instances, skin testing or in vitro testing and drug challenges. The most effective strategy for the management of allergist-confirmed drug allergy is avoidance or discontinuation of the offending drug. When available, alternative medications with unrelated chemical structures should be substituted. Cross-reactivity among drugs should also be taken into consideration when choosing alternative agents. Additional therapy for drug HSRs may include topical corticosteroids, oral antihistamines and, in severe cases, systemic corticosteroids and other immunomodulators. In the event of anaphylaxis, the treatment of choice is intramuscular epinephrine. If a patient with a history of anaphylaxis requires a specific drug and there is no acceptable alternative, desensitization to that drug may be considered. This article provides a background on drug allergy and strategies for the diagnosis and management of some of the most common drug-induced allergic reactions.
Glutamine-glutamate centered metabolism as the potential therapeutic target against Japanese encephalitis virus-induced encephalitis
Authors: Li Mengyuan , Yuan Hang , Yang Xiaofei , Lei Yingfeng , Lian Jianqi 
Published: January 2025

Abstract

Background
Japanese encephalitis (JE) induced by Japanese encephalitis virus (JEV) infection is the most prevalent diagnosed epidemic viral encephalitis globally. The underlying pathological mechanisms remain largely unknown. Given that viruses are obligate intracellular parasites, cellular metabolic reprogramming triggered by viral infection is intricately related to the establishment of infection and progression of disease. Therefore, uncovering and manipulating the metabolic reprogramming that underlies viral infection will help elucidate the pathogenic mechanisms and develop novel therapeutic strategies.

Methods
Metabolomics analysis was performed to comprehensively delineate the metabolic profiles in JEV-infected mice brains and neurons. Metabolic flux analysis, quantitative real-time PCR, western blotting and fluorescence immunohistochemistry were utilized to describe detailed glutamine-glutamate metabolic profiles during JEV infection. Exogenous addition of metabolites and associated compounds and RNA interference were employed to manipulate glutamine-glutamate metabolism to clarify its effects on viral replication. The survival rate, severity of neuroinflammation, and levels of viral replication were assessed to determine the efficacy of glutamine supplementation in JEV-challenged mice.

Results
Here, we have delineated a novel perspective on the pathogenesis of JE by identifying an aberrant low flux in glutamine-glutamate metabolism both in vivo and in vitro, which was critical in the establishment of JEV infection and progression of JE. The perturbed glutamine-glutamate metabolism induced neurotransmitter imbalance and created an immune-inhibitory state with increased gamma-aminobutyric acid/glutamate ratio, thus facilitating efficient viral replication both in JEV-infected neurons and the brain of JEV-infected mice. In addition, viral infection restrained the utilization of glutamine via the glutamate-α-ketoglutaric acid axis in neurons, thus avoiding the adverse effects of glutamine oxidation on viral propagation. As the conversion of glutamine to glutamate was inhibited after JEV infection, the metabolism of glutathione (GSH) was simultaneously impaired, exacerbating oxidative stress in JEV-infected neurons and mice brains and promoting the progression of JE. Importantly, the supplementation of glutamine in vivo alleviated the intracranial inflammation and enhanced the survival of JEV-challenged mice.

Conclusion
Altogether, our study highlights an aberrant glutamine-glutamate metabolism during JEV infection and unveils how this facilitates viral replication and promotes JE progression. Manipulation of these metabolic alterations may potentially be exploited to develop therapeutic approaches for JEV infection.

Graphical Abstract