bluuebunny/crossref_metadata_2025_split huggingface dataset:
A comprehensive review of COVID-19 symptoms and treatments in the setting of autoimmune diseases
Authors: Hamidi Zahra , Jabraeili-Siahroud Shaghaiegh , Taati-Alamdari Yalda , Aghbash Parisa Shiri , Shamekh Ali , Baghi Hossein Bannazadeh 
Published: January 2023

AbstractAfter the first reporting of the index case of Severe Acute Respiratory Syndrome (SARS)-CoV-2-associated disease at the end of December 2019, the virus spread quickly throughout the world, prompting the WHO on 11 March 2020 to declare the disease a global pandemic. The coronavirus disease 2019 (COVID-19) pandemic, raises concerns for all people, mainly for susceptible population. People with pre-existing diseases, especially individuals with autoimmune disorders, are more at the risk of SARS-CoV-2 infection because of compromised immune system due to frequent use of immunosuppressive drugs and steroids. Patients with autoimmune diseases and their physicians have concerns about these patients’ healthcare, since they are at a higher risk for COVID-19 infection, may show severe complications of COVID-19, and may experience probable flares of their pre-existing disease. Even though there have been several studies discussing the relation between COVID-19 and various types of autoimmune diseases, it cannot be ascertained that all patients with autoimmune diseases experience more severe complications of COVID-19 and have more hospitalization or mortality rate. The situation depends on each patient’s condition, such as the type and the severity of the underlying autoimmune disease and the kind of treatment they receive. In the present review, we have discussed the effects of COVID-19 pandemic on patients with different autoimmune diseases and their relative concerns about their treatments. As a result, we have reviewed further considerations that should be taken into account for these patients during the pandemic or when they are infected with COVID-19.
Stingless bees: uses and management by meliponiculturist women in the Chaco region of Bolivia
Authors: Adler Marcia , Escóbar-Márquez Luciana , Solis-Soto Maria Teresa , Pinto Carlos F. 
Published: January 2023

Abstract
Background
Stingless bees have a great value as main pollinators of wild flowering and cultivated plants, thus playing a fundamental role in the maintenance of biodiversity and food security in Latin America. Despite their importance, stingless bees face numerous threats causing alarming population declines. Moreover, stingless bees have a great cultural and traditional value, since most products from the hive are used for a wide variety of purposes. A growing number of initiatives are encouraging the breeding of these bees, through training courses and modern management techniques. This study documents the knowledge on stingless bees and their products that meliponiculturists from the Chaco region of Bolivia have, as well as the influence that meliponiculture initiatives have on the management and general knowledge of the bees.

Methods
Local richness and diversity of stingless bees was calculated using Hill numbers. Structured interviews were conducted with 59 meliponiculturists in order to characterize traditional and formal knowledge on stingless bees and meliponiculture. Generalized linear models were applied to assess the influence of training courses on the management of the bees. Also, a relative cultural importance index was calculated for each species.

Results
Twelve Meliponini species were identified, and 15 local names were reported with morphological, defensiveness behavior, and nest description. There was no significant difference in the knowledge between different ethnical backgrounds or ages. A significant difference was observed in the use of supplementary feeding and assisted division, but none in the success in racking hives or in pest management, regarding the number or courses taken. The relative cultural importance index recorded 30 specific uses for bee products grouped in four categories, from which 29 were attributed (but not exclusively) to Tetragonisca angustula, making it the most versatile species.

Conclusions
The products of the hive, especially honey, are used for a wide variety of purposes, mostly in medicine. These uses are mostly attributed to just one species, T. angustula, in coincidence with what was taught in meliponiculture training courses by NGOs. The influence of formal knowledge is mostly positive, but it is recommended that other meliponini species are taken into account as well.
Representation of women in orthopaedic surgery: perception of barriers among undergraduate medical students in Saudi Arabia
Authors: Alomar Abdulaziz Z. , Almonaie Shahd , Nagshabandi Khalid Nabil , AlGhufaili Deema , Alomar Manar 
Published: January 2023

Abstract
Background
While female participation has improved in several surgical specialties over time globally, no such increase has been observed in orthopaedic surgery over the past decades. The potential barriers to female participation are likely present from the beginning of medical education. Therefore, this study assessed the apparent lag in equal representation among men and women in orthopaedic surgery in the Kingdom of Saudi Arabia.

Methods
This cross-sectional study used a questionnaire survey to investigate medical students’ and interns’ perceptions of women participating in orthopaedic surgery, their subspeciality preferences, and barriers preventing them from pursuing an orthopaedic career. The responses were analysed to understand general perceptions, gender-based differences, impact of clinical experiential learning, and exposure to orthopaedic surgery.

Results
Approximately 565 medical students (49% females, 51% males) participated in the survey. Only 17% of students (11% females, 23% males) considered orthopaedic surgery as their future career option. While 31% of female and 17% of male students disagreed with the concept of female-appropriate orthopaedic subspecialties, most of the remaining male and female students perceived paediatric orthopaedics as a female-appropriate subspecialty. Concerning equal representation of women, gender bias and lack of a strong physique were the most frequently selected barriers by female and male students, respectively. Patient preference for male orthopaedicians, gender discrimination, social and family commitments, and need for physical strength were all perceived as barriers for women in orthopaedics. Overall, clinical experience and orthopaedic exposure did not significantly improve the likelihood of female students in choosing orthopaedic surgery as a career.

Conclusions
The bias against women in orthopaedic careers is prevalent among medical students early in their academic years. Clinical experience and exposure to orthopaedic surgery should be improved to make a significant impact on female participation in orthopaedic careers. Career building efforts in terms of improved career opportunities, career counselling, flexible working hours, social and family related adjustments and implementation of mentorship/research/fellowship programmes for females are needed to reduce gender discrimination and improve female orthopaedic participation. Furthermore, process improvements may yield greater flexibility for women pursuing the challenging field while accommodating other barriers faced by women in orthopaedic surgery.
Extru-seq: a method for predicting genome-wide Cas9 off-target sites with advantages of both cell-based and in vitro approaches
Authors: Kwon Jeonghun , Kim Minyoung , Hwang Woochang , Jo Anna , Hwang Gue-Ho , Jung Minhee , Kim Un Gi , Cui Gang , Kim Heonseok , Eom Joon-Ho , Hur Junho K. , Lee Junwon , Kim Youngho , Kim Jin-soo , Bae Sangsu , Lee Jungjoon K. 
Published: January 2023

AbstractWe present a novel genome-wide off-target prediction method named Extru-seq and compare it with cell-based (GUIDE-seq), in vitro (Digenome-seq), and in silico methods using promiscuous guide RNAs with large numbers of valid off-target sites. Extru-seq demonstrates a high validation rate and retention of information about the intracellular environment, both beneficial characteristics of cell-based methods. Extru-seq also shows a low miss rate and could easily be performed in clinically relevant cell types with little optimization, which are major positive features of the in vitro methods. In summary, Extru-seq shows beneficial features of cell-based and in vitro methods.
Differential CpG DNA methylation of peripheral B cells, CD4+ T cells, and salivary gland tissues in IgG4-related disease
Authors: Wu Xunyao , Wang Anqi , Wang Mu , Peng Yu , Chen Yingying , Li Jieqiong , Liu Zheng , Lu Hui , Zhou Jiaxin , Peng Linyi , Zhao Yan , Zeng Xiaofeng , Fei Yunyun , Zhang Wen 
Published: January 2023

Abstract
Objectives
Immunoglobulin-G4-related disease (IgG4-RD) is a distinct systemic autoimmune-mediated disease manifesting as chronic inflammation and tissue fibrosis. Since the role of DNA methylation in the pathogenesis of IgG4-RD is still unclear, we conduct this study to investigate epigenetic modifications in IgG4-RD.

Methods
A genome-wide DNA methylation study was conducted with B cells, CD4+ T cells, and salivary gland tissues from IgG4-RD patients and matched controls by using the Illumina HumanMethylation 850K BeadChip. We further performed pyrosequencing and immunohistochemistry assays to validate the methylation status of some targets of interest.

Results
We identified differentially methylated CpG sites including 44 hypomethylated and 166 hypermethylated differentially methylated probes (DMPs) in B cells and 260 hypomethylated and 112 hypermethylated DMPs in CD4+ T cells from 10 IgG4-RD patients compared with 10 healthy controls. We also identified 36945 hypomethylated and 78380 hypermethylated DMPs in salivary gland tissues of 4 IgG4-RD patients compared with 4 controls. DPM2 (cg21181453), IQCK (cg10266221), and ABCC13 (cg05699681, cg04985582) were hypermethylated and MBP (cg18455083) was hypomethylated in B cells, CD4+ T cells, and salivary gland tissues of IgG4-RD patients. We also observed the hypomethylated HLA-DQB2 in CD4+ T cells from IgG4-RD patients. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis of DMPs in salivary gland tissues of IgG4-RD patients revealed enrichment of pathways involved in the regulation of immune cell responses and fibrosis.

Conclusion
This is the first DNA methylation study in peripheral B cells, CD4+ T cells, and salivary gland tissues from IgG4-RD patients. Our findings highlighted the role of epigenetic modification of DNA methylation and identified several genes and pathways possibly involved in IgG4-RD pathogenesis.
Continuity of care (COC) and amyloid-β PET scan: the CARE-IDEAS study
Authors: O’Brien Emily C. , Ford Cassie B. , Sorenson Corinna , Jutkowitz Eric , Shepherd-Banigan Megan , Van Houtven Courtney 
Published: January 2023

Abstract
Background
High continuity of care (COC) is associated with better clinical outcomes among older adults. The impact of amyloid-β PET scan on COC among adults with mild cognitive impairment (MCI) or dementia of uncertain etiology is unknown.

Methods
We linked data from the CARE-IDEAS study, which assessed the impact of amyloid-β PET scans on outcomes in Medicare beneficiaries with MCI or dementia of uncertain etiology and their care partners, to Medicare claims (2015–2018). We calculated a participant-level COC index using the Bice-Boxerman formula and claims from all ambulatory evaluation and management visits during the year prior to and following the amyloid-β PET scan. We compared baseline characteristics by scan result (elevated or non-elevated) using standardized differences. To evaluate changes in COC, we used multiple regression models adjusting for sociodemographics, cognitive function, general health status, and the Charlson Comorbidity Index.

Results
Among the 1171 cohort members included in our analytic population, the mean age (SD) was 75.2 (5.4) years, 61.5% were male and 93.9% were non-Hispanic white. Over two-thirds (68.1%) had an elevated amyloid-β PET scan. Mean COC for all patients was 0.154 (SD = 0.102; range = 0–0.73) prior to the scan and 0.158 (SD = 0.105; range = 0–1.0) in the year following the scan. Following the scan, the mean COC index score increased (95% CI) by 0.005 (−0.008, 0.019) points more for elevated relative to not elevated scan recipients, but this change was not statistically significant. There was no association between scan result (elevated vs. not elevated) or any other patient covariates and changes in COC score after the scan.

Conclusion
COC did not meaningfully change following receipt of amyloid-β PET scan in a population of Medicare beneficiaries with MCI or dementia of uncertain etiology. Future work examining how care continuity varies across marginalized populations with cognitive impairment is needed.
Bilateral collagenous fibroma of the hard palate: a case report and review of the literature
Authors: El-naggar Hagar A. , El-Mahallawy Yehia A. , Harby Mohamed I. , Abou Madawi Nourhan A. 
Published: January 2023

Abstract
Background
Collagenous fibroma or desmoplastic fibroblastoma is a rare benign fibrous tissue tumor. It usually presents as a painless, slowly growing mass. Collagenous fibroma arises ordinarily inside the subcutaneous tissues or skeletal muscles. Histopathologically, the tumor consists of scattered stellate and spindle cells in a hypovascular collagenous stroma without atypia or infiltration. The oral cavity is a very uncommon site for desmoplastic fibroblastoma. Only 15 published articles in the literature reported the intraoral location. We present a case of collagenous fibroma with a bilateral distribution on the hard palate. This is the second case of bilateral collagenous fibroma after a previously reported one in literature; however, our case was larger, occupying almost the whole palate. We discuss the management of this rare tumor and how we can reach definite diagnosis.

Case presentation
A 37-year-old Caucasian female patient had a huge bilateral firm palatal mass that caused breathing problems. There was no history of trauma and the patient had no relevant medical history Total surgical excision under general anesthesia was carried out and histopathological examination suggested a benign mesenchymal tumor. Immunohistochemistry was necessary to confirm the tumor origin and to exclude aggressive fibromatosis. A diagnosis of bilateral collagenous fibroma was reached. Six months after surgery, there was no recurring lesion and the patient’s health was good.

Conclusions
Collagenous fibroma is a benign fibrous tissue tumor of unknown cause that is treated with simple excision. The prognosis is good with no recurrence. Reaching an accurate diagnosis is mandatory to avoid aggressive treatment since collagenous fibroma may be misdiagnosed as aggressive fibromatosis in case of massive size. Clinicians and pathologists should be aware of this unusual tumor for conservative management without side effects.
Addictive brain-network identification by spatial attention recurrent network with feature selection
Authors: Gong Changwei , Chen Xinyi , Mughal Bushra , Wang Shuqiang 
Published: January 2023

AbstractAddiction in the brain is associated with adaptive changes that reshape addiction-related brain regions and lead to functional abnormalities that cause a range of behavioral changes, and functional magnetic resonance imaging (fMRI) studies can reveal complex dynamic patterns of brain functional change. However, it is still a challenge to identify functional brain networks and discover region-level biomarkers between nicotine addiction (NA) and healthy control (HC) groups. To tackle it, we transform the fMRI of the rat brain into a network with biological attributes and propose a novel feature-selected framework to extract and select the features of addictive brain regions and identify these graph-level networks. In this framework, spatial attention recurrent network (SARN) is designed to capture the features with spatial and time-sequential information. And the Bayesian feature selection(BFS) strategy is adopted to optimize the model and improve classification tasks by restricting features. Our experiments on the addiction brain imaging dataset obtain superior identification performance and interpretable biomarkers associated with addiction-relevant brain regions.
Grading assessment of steel bar corrosion in concrete structures based on naive Bayesian model and spontaneous magnetic flux leakage effect
Authors: Li Beian , Li Shidong , Zhang Guangzhao , Ying Huajian , Xia Qianwen , Liu Shangkai 
Published: January 2023

AbstractIn order to explore an accurate method to evaluate the corrosion of reinforced concrete structures, the spontaneous magnetic flux leakage (SMFL) signal distribution on the surface of reinforced concrete specimens under different corrosion degrees was scanned based on SMFL technology. The influence of steel bar length, steel bar diameter, and other parameters on the distribution of SMFL signal was studied. The correlation between steel bar corrosion and the characteristic magnetic index of concrete structure was explored. Based on the naive Bayesian model, the classification evaluation of the steel bar corrosion degree of concrete structure was carried out. The results show that the variation of SMFL signal is affected by the corrosion degree α. When the lift-off height and the thickness of concrete protective layer remain unchanged, the slope between the peak and trough of Bz (magnetic induction intensity along z direction) curve increases with the increase of α, and the trough of Bx (magnetic induction intensity along x direction) curve decreases with the increase of the corrosion degree α. The peak and trough of magnetic signal curve can be used as the basis for determining the corrosion position. There is a strong correlation between the magnetic characteristic index β, γ, and the steel corrosion degree α obtained by SMFL. Through the characterization relationship between α, β, and γ, the corresponding models of single and comprehensive index β and γ were established. The results showed that the accuracy of β and γ integrated discriminant Naive Bayesian model-III reached 90.7%, which proved that the evaluation method has high reliability. This study explores the application of SMFL in corrosion detection of concrete structures.
Lessons from CDER’s Quality Management Maturity Pilot Programs
Authors: Maguire Jennifer , Fisher Adam , Harouaka Djamila , Rakala Nandini , Lundi Carla , Yambot Marcus , Viehmann Alex , Stiber Neil , Gonzalez Kevin , Canida Lyle , Buhse Lucinda , Kopcha Michael 
Published: January 2023

Abstract
Between October 2020 and March 2022, FDA’s Center for Drug Evaluation and Research (CDER) completed two pilot programs to assess the quality management maturity (QMM) of drug manufacturing establishments. Mature quality systems promote proactive detection of vulnerabilities, prevent problems before they occur, and foster a culture that rewards process and system improvements. A CDER QMM program may help to advance supply chain resiliency and robustness and mitigate drug shortages. One pilot program evaluated seven establishments located within the U.S. that produce finished dosage form products marketed in the U.S. A second pilot program evaluated eight establishments located outside the U.S. that produce active pharmaceutical ingredients used in drug products marketed in the U.S. The execution of these pilot programs afforded FDA the opportunity to learn important lessons about the establishment QMM assessment process, scoring approach, assessor behaviors, and perceptions of the assessment questions, reports, and ratings. Many of the participating establishments reported that the QMM pilot assessments helped to identify their strengths, weaknesses, and new areas for improvement which they had not previously identified through internal audits or CGMP inspections. There has been a great deal of interest in the outcomes of CDER’s QMM pilot programs and this paper describes, for the first time, the lessons CDER learned and will continue to heed in the development of a QMM program.
Graphical Abstract
Coconut Oil Saturated Fatty Acids Improved Energy Homeostasis but not Blood Pressure or Cognition in VCD-Treated Female Mice
Authors: Sui Ke , Yasrebi Ali , Longoria Candace R , MacDonell Avery T , Jaffri Zehra H , Martinez Savannah A , Fisher Samuel E , Malonza Natasha , Jung Katie , Tveter Kevin M , Wiersielis Kimberly R , Uzumcu Mehmet , Shapses Sue A , Campbell Sara C , Roepke Troy A , Roopchand Diana E 
Published: January 2023

Abstract
Obesity, cardiometabolic disease, cognitive decline, and osteoporosis are symptoms of postmenopause, which can be modeled using 4-vinylcyclohexene diepoxide (VCD)–treated mice to induce ovarian failure and estrogen deficiency combined with high-fat diet (HFD) feeding. The trend of replacing saturated fatty acids (SFAs), for example coconut oil, with seed oils that are high in polyunsaturated fatty acids, specifically linoleic acid (LA), may induce inflammation and gut dysbiosis, and worsen symptoms of estrogen deficiency. To investigate this hypothesis, vehicle (Veh)- or VCD-treated C57BL/6J mice were fed a HFD (45% kcal fat) with a high LA:SFA ratio (22.5%: 8%), referred to as the 22.5% LA diet, or a HFD with a low LA:SFA ratio (1%: 31%), referred to as 1% LA diet, for a period of 23 to 25 weeks. Compared with VCD-treated mice fed the 22.5% LA diet, VCD-treated mice fed the 1% LA diet showed lower weight gain and improved glucose tolerance. However, VCD-treated mice fed the 1% LA diet had higher blood pressure and showed evidence of spatial cognitive impairment. Mice fed the 1% LA or 22.5% LA diets showed gut microbial taxa changes that have been associated with a mix of both beneficial and unfavorable cognitive and metabolic phenotypes. Overall, these data suggest that consuming different types of dietary fat from a variety of sources, without overemphasis on any particular type, is the optimal approach for promoting metabolic health regardless of estrogen status.