bluuebunny/crossref_metadata_2025_split huggingface dataset:
Nasogastric tube insertion length measurement and tip verification in adults: a narrative review
Authors: Boeykens Kurt , Holvoet Tom , Duysburgh Ivo 
Published: August 2023

AbstractNasogastric feeding tube insertion is a common but invasive procedure most often blindly placed by nurses in acute and chronic care settings. Although usually not harmful, serious and fatal complications with misplacement still occur and variation in practice still exists. These tubes can be used for drainage or administration of fluids, drugs and/or enteral feeding. During blind insertion, it is important to achieve correct tip position of the tube ideally reaching the body of the stomach. If the insertion length is too short, the tip and/or distal side-openings at the end of the tube can be located in the esophagus increasing the risk of aspiration (pneumonia). Conversely, when the insertion length is too long, the tube might kink in the stomach, curl upwards into the esophagus or enter the duodenum. Studies have demonstrated that the most frequently used technique to determine insertion length (the nose–earlobe–xiphoid method) is too short a distance; new safer methods should be used and further more robust evidence is needed. After blind placement, verifying correct gastric tip positioning is of major importance to avoid serious and sometimes lethal complications.
Dysbiosis of gut microbiota during fecal stream diversion in patients with colorectal cancer
Authors: Lee Soo Young , Park Hyeung-Min , Kim Chang Hyun , Kim Hyeong Rok 
Published: August 2023

Abstract
Background
The effect of fecal stream diversion on the gut microbiota is still uncertain. The present study was designed to assess the effect of fecal stream diversion on the composition of the gut microbiota in patients with colorectal cancer. We included patients undergoing left-sided colorectal cancer surgery with (ileostomy group) or without (control group) diverting ileostomy. Fecal samples were collected from 10 patients in each group before surgery (t1) and after ileostomy repair in the ileostomy group and 6–12 months after the initial surgery in the control group (t2). The fecal microbiota was assessed using 16S rRNA sequencing, and changes in the composition of the fecal microbiota were compared between the two groups.

Results
Alpha diversity analysis revealed that the complexity of fecal microbiota decreased between t1 and t2 only in the ileostomy group. Beta diversity analysis also showed dissimilarity between t1 and t2 only in the ileostomy group. The composition of the microbiota was similar between the two groups at t1. However, at t2, the ileostomy group had lower proportion of beneficial bacteria (Lachnospiraceae, 3.8% vs. 29.9%, p < 0.001; Ruminococcaceae, 0.6% vs. 18.4%, p < 0.001; Blautia, 0.1% vs. 9.1%, p < 0.001; Faecalibacterium, 0.2% vs. 7.5%, p < 0.001) and a higher proportion of harmful bacteria (Proteobacteria, 17.9% vs. 5.1%, p = 0.006; Clostridium, 16.2% vs. 1.1%, p = 0.013; Streptococcus, 17.7% vs. 1.6%, p = 0.002) than the control group.

Conclusions
Fecal stream diversion was closely associated with less diversity and dysbiosis of the gut microbiota.
Pastoralism in South Asia: Contemporary stresses and adaptations of Himalayan pastoralists
Authors: Singh Rashmi , Kerven Carol 
Published: August 2023

AbstractWe discuss the main findings in the Special issue on Pastoralism in South Asia from the eight papers based on research conducted in the Himalayan region of South Asia. An overview is presented of pastoralism in the Himalayan region, including India, Pakistan, Nepal and Bhutan. Drawing parallels with the global stresses to pastoralists, papers in this special issue highlighted three sets of contemporary stresses to the pastoralists of the Himalayan region viz. (a) lack of herding labour, associated changing aspirations of youth and decline in traditional knowledge systems; (b) continued stresses from the state and between the formal and informal institutions; and (c) climatic stresses and associated impacts on the rangeland and livestock health. A synthesis of findings from all eight case studies suggests how the issues around pastoral livelihoods and rangeland management in the Himalayan region are entangled across social, political and ecological dimensions. However, instead of only being impacted by the stresses, the pastoral communities are showing adaptations to various kinds of uncertainties and variabilities. Based on these findings from across eight sites in the Himalayan region, we argue that understanding of the problems as well as proposed solutions from the policymakers should be tailored according to the particular social, political and ecological contexts. Other than the issues given prominence in this special issue, the role of markets and social security are some of the other important concerns to be addressed in the region, which can be best addressed by creating an interface between pastoralists and policymakers, practitioners and the government, while making the best use of pastoral knowledge and their way of life.
HIF1A-repressed PUS10 regulates NUDC/Cofilin1 dependent renal cell carcinoma migration by promoting the maturation of miR-194-5p
Authors: Luo Wenqin , Xu Zhehao , Wang Huan , Lu Zeyi , Ding Lifeng , Wang Ruyue , Xie Haiyun , Zheng Qiming , Lin Yudong , Zhou Zhenwei , Li Yang , Chen Xianjiong , Li Gonghui , Xia Liqun 
Published: August 2023

Abstract
Background
Renal cell carcinoma (RCC) is characterized by a high rate of distant metastasis, which leads to poor prognosis in patients with advanced RCC. PUS10 has been recognized as a member of the pseudouridine synthase family, and recently other functions beyond the synthesis of the RNA modification have been uncovered. However, little is known about its role in diseases such as cancer.

Methods
RT-qPCR, western blot and immunohistochemistry were used to measure the expression of PUS10 in RCC tissues. Transwell assay, wound healing assay, and in vivo metastasis model were conducted to determine the function of PUS10 in RCC progression. MicroRNA sequencing and GEO database were used to screen for the downstream microRNAs of PUS10. RNA immunoprecipitation, dual luciferase reporter assay, immunostaining, and rescue experiments were employed to establish the PUS10/miR-194-5p/nuclear distribution protein C(NUDC)/Cofilin1 axis in RCC migration. Chromatin immunoprecipitation and dual luciferase reporter assay were used to verify its upstream transcriptional regulator.

Results
The expression of PUS10 was significantly decreased in RCC tissues, and low expression predicted poor prognosis. In vitro and in vivo experiments showed that PUS10 suppressed RCC migration, which, however, was independent of its classical pseudouridine catalytic function. Mechanically, PUS10 promoted the maturation of miR-194-5p, which sequentially inhibited RCC migration via disrupting NUDC-dependent cytoskeleton. Furthermore, hypoxia and HIF-1 A were found involved in the downregulation of PUS10.

Conclusion
We unraveled PUS10 restrained RCC migration via the PUS10/miR-194-5p/NUDC/Cofilin1 pathway, which independent of its classical catalytic function. Furthermore, a linkage between the critical tumor microenvironment hallmark with malfunction of the forementioned metastasis inhibition mechanism was presented, as demonstrated by repressed expression of PUS10 due to hypoxia and HIF-1A.
Evaluation of the safety and efficacy of extracorporeal carbon dioxide removal in the critically ill using the PrismaLung+ device
Authors: Tiruvoipati Ravindranath , Ludski Jarryd , Gupta Sachin , Subramaniam Ashwin , Ponnapa Reddy Mallikarjuna , Paul Eldho , Haji Kavi 
Published: August 2023

Abstract
Background
Several extracorporeal carbon dioxide removal (ECCO2R) devices are currently in use with variable efficacy and safety profiles. PrismaLung+ is an ECCO2R device that was recently introduced into clinical practice. It is a minimally invasive, low flow device that provides partial respiratory support with or without renal replacement therapy. Our aim was to describe the clinical characteristics, efficacy, and safety of PrismaLung+ in patients with acute hypercapnic respiratory failure.

Methods
All adult patients who required ECCO2R with PrismaLung+ for hypercapnic respiratory failure in our intensive care unit (ICU) during a 6-month period between March and September 2022 were included.

Results
Ten patients were included. The median age was 55.5 (IQR 41–68) years, with 8 (80%) male patients. Six patients had acute respiratory distress syndrome (ARDS), and two patients each had exacerbations of asthma and chronic obstructive pulmonary disease (COPD). All patients were receiving invasive mechanical ventilation at the time of initiation of ECCO2R. The median duration of ECCO2R was 71 h (IQR 57–219). A significant improvement in pH and PaCO2 was noted within 30 min of initiation of ECCO2R. Nine patients (90%) survived to weaning of ECCO2R, eight (80%) survived to ICU discharge and seven (70%) survived to hospital discharge. The median duration of ICU and hospital stays were 14.5 (IQR 8–30) and 17 (IQR 11–38) days, respectively. There were no patient-related complications with the use of ECCO2R. A total of 18 circuits were used in ten patients (median 2 per patient; IQR 1–2). Circuit thrombosis was noted in five circuits (28%) prior to reaching the expected circuit life with no adverse clinical consequences.

Conclusion(s)
PrismaLung+ rapidly improved PaCO2 and pH with a good clinical safety profile. Circuit thrombosis was the only complication. This data provides insight into the safety and efficacy of PrismaLung+ that could be useful for centres aspiring to introduce ECCO2R into their clinical practice.
Spatial pattern of arsenic concentration and associated noncarcinogenic health risk assessment: a case study on Gangni Union of Chuadanga district of Bangladesh
Authors: Islam Md. Nazrul , Haque Md. Inzamul , Hossain Md. Anik 
Published: August 2023

AbstractGroundwater is one of the world’s most important sources of fresh drinking water. Various contaminants mix with groundwater and alter its natural composition, such as arsenic. This study aimed to ascertain the present condition of arsenic concentration, its spatial pattern, and its relationship with tube well depth in the Gangni Union in the Chuadanga district of Bangladesh. Additionally, the study tried to assess the associated noncarcinogenic health risks imposed by oral ingestion of arsenic. Systematic sampling was used to collect water samples $$(n=100)$$

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along with depth information from the sample tube wells. Water samples were analyzed with the pre-calibrated Hach EZ, Dual-Range Arsenic Test Kit (Range: 0.00–0.5 mg/l). Both geostatistical (spatial autocorrelation, Hotspot analysis, and IDW) and statistical (descriptive and correlation statistics) methods were used. The resultant arsenic content of the samples tested ranges from 0.0004 (mg/l) to 0.10 (mg/l). Arsenic levels in almost 42% of the samples exceeded the WHO standard, 21% exceeded the Bangladesh standard, and 37% were within the tolerable standard. Geostatistical analysis shows that approximately 63% of the total area is arsenic contaminated. Furthermore, hotspot analysis reveals that the northeastern and southeastern parts of the study area are more arsenic-contaminated than the other parts. Noncarcinogenic health risk assessment shows that children have a higher average daily dose (ADD) range (8.33E-06-0.00181) than adults (2.78E-06-0.0006). Similarly, the hazard quotient (HQ) value is also higher for children (0.0277–6.033) than for adults (0.0092–2.011). The result of Pearson’s correlation coefficient, r (98) = − 0.7580, p = 0.000, shows a negative linear relationship between concentration values and depth, meaning that increasing depth will reduce arsenic contamination from tube well water.
Interaction of surfactants with Prunus laurocerasus leaf surfaces: time-dependent recovery of wetting contact angles depends on physico-chemical properties of surfactants
Authors: Baales Johanna , Zeisler-Diehl Viktoria V. , Narine Suraya , Schreiber Lukas 
Published: August 2023

Abstract
Background
Surfactants are added to spray solutions because they significantly improve foliar uptake of active ingredients (AIs) into the leaves. It was intended to investigate whether surfactant solutions forming a dried deposit on Prunus leaf surfaces after they were sprayed, lead to structural and functional changes of the cuticle/atmosphere interface. This could potentially result in irreversibly enhanced leaf surface wetting, which should be of major disadvantage. Enhanced wetting could promote leaching of ions and promote leaf surface colonization with microorganisms.

Results
Prunus laurocerasus leaf surfaces were sprayed with aqueous solutions of non-ionic alcohol ethoxylates, a cationic, an anionic and one large polar surfactant. Directly after spraying and drying of the different surfactant solutions, wetting contact angles of deionized water (without surfactant) were significantly lower (between 6 and 54°) compared to wetting contact angles on untreated leaves (77°). Leaf surface wettability with deionized water was more pronounced with non-ionic alcohol ethoxylates (wetting contact angles ranging between 6 and 22°) compared to the other 3 surfactants (wetting contact angles ranging between 42 and 54°). Wetting contact angles of deionized water on leaf surfaces treated with non-ionic alcohol ethoxylates continuously increased again over time resulting in final wetting contact angles not different from untreated leaf surfaces. The time-dependent recovery of wetting contact angles was dependent on the degree of ethoxylation of the non-ionic alcohol ethoxylates. The wetting contact angle recovery rate was lower the higher the degree of ethoxylation of the alcohol ethoxylates was. With the cationic, anionic and large polar surfactant a recovery of wetting contact angles was not observed. In addition, on fully dehydrated and dead leaves wetting contact angle recovery was not observed for any of the tested surfactants after spraying and drying. Analytical determinations of the amounts of alcohol ethoxylates on the leaf surfaces after spraying and drying showed that amounts of alcohol ethoxylates decreased over time on the surface (24–72 h).

Conclusion
Our results indicate that non-ionic alcohol ethoxylates diffused within hours from the leaf surface into the leaf over time and thus fully disappeared from the leaf surface. This was not the case with the cationic, anionic and the large polar surfactants remaining on the leaf surface.

Graphical Abstract
On the use of tsunami-source data for high-resolution fault imaging of offshore earthquakes
Authors: Kubo Hisahiko , Kubota Tatsuya , Suzuki Wataru , Nakamura Takeshi 
Published: August 2023

AbstractThe source imaging for offshore earthquakes using terrestrial geodetic data has a limited estimation performance due to the low data resolution. One approach to overcome this limitation is the use of seafloor geodetic data. In this study, we focus on tsunami-source data, which is the spatial distribution of vertical crustal displacements above the source area and can be derived from tsunami waveform records. We evaluate how the use of this spatial seafloor geodetic data improves the estimation of a rectangular fault model. Here, the fault model of the 2016 off-Fukushima earthquake in Japan, which was a shallow intraplate earthquake (Mw 7.0), was estimated by three inversions: terrestrial Global Navigation Satellite System (GNSS) data only, tsunami-source data only, and a combination of the GNSS data and tsunami-source data. A Bayesian inversion approach was used to understand the distribution of the estimated fault parameters and their relationship. The results indicated that the terrestrial GNSS data have a low resolution for the analysis of the offshore earthquake, which resulted in a biased solution with large uncertainty. Conversely, the use of tsunami-source data significantly improved the resolution and reliability of source imaging and reduced the dependency among fault parameters. These results suggested that the high-spatial-resolution information of tsunami source is a powerful tool in source imaging of offshore shallow earthquakes. Moreover, the combined use of the two different geodetic data leads to a more robust estimation of fault parameters. We believe that the use of tsunami-source data is useful, not only for the post hoc source analysis, but also for estimating an earthquake rupture area just after a large earthquake, where GNSS data are currently used.
Graphical Abstract
Recent global nonhydrostatic modeling approach without using a cumulus parameterization to understand the mechanisms underlying cloud changes due to global warming
Authors: Noda Akira T. , Ohno Tomoki , Kodama Chihiro , Chen Ying-Wen , Kuba Naomi , Seiki Tatsuya , Yamada Yohei , Satoh Masaki 
Published: August 2023

AbstractClouds are the primary source of uncertainty in the prediction of climate change. To reduce the uncertainty of cloud simulations and overcome this difficulty in prediction, many climate modeling centers are now developing a new type of climate model, the global nonhydrostatic atmospheric model, which reduces the uncertainty arising from a cumulus parameterization by computing clouds explicitly using a cloud microphysics scheme. Among the global nonhydrostatic atmospheric models used in recent intercomparison studies, NICAM aims to project climate change by improving our understanding of cloud changes due to warming and related physical processes. NICAM is the first global nonhydrostatic model and was developed by our research team. This review summarizes the outcomes of a recent major five-year research program in Japan for studying climate using NICAM, as well as providing an overview of current issues regarding the use of global kilometer-scale simulations in high-resolution climate modeling.
Jdp2 is a spatiotemporal transcriptional activator of the AhR via the Nrf2 gene battery
Authors: Wuputra Kenly , Tsai Ming-Ho , Kato Kohsuke , Ku Chia-Chen , Pan Jia-Bin , Yang Ya-Han , Saito Shigeo , Wu Chun-Chieh , Lin Ying-Chu , Cheng Kuang-Hung , Kuo Kung-Kai , Noguchi Michiya , Nakamura Yukio , Yoshioka Tohru , Wu Deng-Chyang , Lin Chang-Shen , Yokoyama Kazunari K. 
Published: August 2023

AbstractBackgroundCrosstalk between the aryl hydrocarbon receptor (AhR) and nuclear factor (erythroid-derived 2)-like 2 (Nrf2) signaling is called the “AhR–Nrf2 gene battery”, which works synergistically in detoxification to support cell survival. Nrf2-dependent phase II gene promoters are controlled by coordinated recruitment of the AhR to adjacent dioxin responsive element (DRE) and Nrf2 recruitment to the antioxidative response element (ARE). The molecular interaction between AhR and Nrf2 members, and the regulation of each target, including phase I and II gene complexes, and their mediators are poorly understood.MethodsKnockdown and forced expression of AhR–Nrf2 battery members were used to examine the molecular interactions between the AhR–Nrf2 axis andAhRpromoter activation. Sequential immunoprecipitation, chromatin immunoprecipitation, and histology were used to identify each protein complex recruited to their respectivecis-elements in theAhRpromoter. Actin fiber distribution, cell spreading, and invasion were examined to identify functional differences in the AhR–Jdp2 axis between wild-type andJdp2knockout cells. The possible tumorigenic role of Jdp2 in the AhR–Nrf2 axis was examined in mutantKras-Trp53-driven pancreatic tumors.ResultsCrosstalk between AhR and Nrf2 was evident at the transcriptional level. TheAhRpromoter was activated by phase I ligands such as 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) through the AhR–Jdp2–Nrf2 axis in a time- and spatial transcription-dependent manner. Jdp2 was a bifunctional activator of DRE- and ARE-mediated transcription in response to TCDD. After TCDD exposure, Jdp2 activated theAhRpromoter at the DRE and then moved to the ARE where it activated the promoter to increase reactive oxygen species (ROS)-mediated functions such as cell spreading and invasion in normal cells, and cancer regression in mutantKras-Trp53-driven pancreatic tumor cells.ConclusionsJdp2 plays a critical role inAhRpromoter activation through the AhR–Jdp2–Nrf2 axis in a spatiotemporal manner. The AhR functions to maintain ROS balance and cell spreading, invasion, and cancer regression in a mouse model of mutantKras–Trp53pancreatic cancer. These findings provide new insights into the roles of Jdp2 in the homeostatic regulation of oxidative stress and in the antioxidation response in detoxification, inflammation, and cancer progression.
Age and sex dependent differences in midline facial soft tissue thicknesses measured on MRI scans of Northwest Indian subjects: a forensic anthropological study
Authors: Thakur Shubham , Sehrawat J. S. 
Published: August 2023

Abstract
Background
Human face is complex and intricate structure, with several features that are unique to each individual and can be taken as an identity pass in this mortal world. The facial profile of an individual holds materialistic value and is crucial for human identification. Present study aims to investigate the influence of sex and age of an individual on the soft tissue thicknesses (FSTT) at midline facial landmarks estimated on MRI scans.

Methods
Facial soft tissue thickness was estimated at 20 midline landmarks on the MRI scans of 120 (57 male; 63 female) anatomically normal Northwest Indian adult subjects (18–70 + years age-group). Age and sex dependent variations in tissue thicknesses at different landmarks were estimated using statistical software IBM SPSS Statistics 23.

Results
The results of the study yielded that most of the midline landmarks had higher values in males than in females indicating observable sexual dimorphism in FSTT values in this facial region, statistically significant differences were also noted in soft tissue thickness estimates of different age-groups, showing increasing or decreasing trend with age. The mid-facial landmarks like mid-philtrum, supra-dentale, incisor superius, and labile inferius displayed highly significant p values.

Conclusions
Present study results were in tandem with the findings of previous studies; males and younger age-group individuals were having higher tissue thickness values than the females and older individuals, respectively. The highest soft tissue estimates were obtained for the middle age subjects and the most aged individuals had the least midline soft tissue thickness values. These results can have significant forensic anthropological repercussions in attempting facial approximations on unknown skulls and identification of unknown individuals from still images, photographs or videos.