bluuebunny/crossref_metadata_2025_split huggingface dataset:
Associations of height, body mass index, and weight gain with breast cancer risk in carriers of a pathogenic variant in BRCA1 or BRCA2: the BRCA1 and BRCA2 Cohort Consortium
Authors: Kast Karin , John Esther M. , Hopper John L. , Andrieu Nadine , Noguès Catherine , Mouret-Fourme Emmanuelle , Lasset Christine , Fricker Jean-Pierre , Berthet Pascaline , Mari Véronique , Salle Lucie , Schmidt Marjanka K. , Ausems Margreet G. E. M. , Garcia Encarnacion B. Gomez , van de Beek Irma , Wevers Marijke R. , Evans D. Gareth , Tischkowitz Marc , Lalloo Fiona , Cook Jackie , Izatt Louise , Tripathi Vishakha , Snape Katie , Musgrave Hannah , Sharif Saba , Murray Jennie ,   EMBRACE Collaborators, Colonna Sarah V. , Andrulis Irene L. , Daly Mary B. , Southey Melissa C. , de la Hoya Miguel , Osorio Ana , Foretova Lenka , Berkova Dita , Gerdes Anne-Marie , Olah Edith , Jakubowska Anna , Singer Christian F. , Tan Yen , Augustinsson Annelie , Rantala Johanna , Simard Jacques , Schmutzler Rita K. , Milne Roger L. , Phillips Kelly-Anne , Terry Mary Beth , Goldgar David , van Leeuwen Flora E. , Mooij Thea M. , Antoniou Antonis C. , Easton Douglas F. , Rookus Matti A. , Engel Christoph 
Published: June 2023

AbstractIntroductionHeight, body mass index (BMI), and weight gain are associated with breast cancer risk in the general population. It is unclear whether these associations also exist for carriers of pathogenic variants in theBRCA1orBRCA2genes.Patients and methodsAn international pooled cohort of 8091BRCA1/2variant carriers was used for retrospective and prospective analyses separately for premenopausal and postmenopausal women. Cox regression was used to estimate breast cancer risk associations with height, BMI, and weight change.ResultsIn the retrospective analysis, taller height was associated with risk of premenopausal breast cancer forBRCA2variant carriers (HR 1.20 per 10 cm increase, 95% CI 1.04–1.38). Higher young-adult BMI was associated with lower premenopausal breast cancer risk for bothBRCA1(HR 0.75 per 5 kg/m2, 95% CI 0.66–0.84) andBRCA2(HR 0.76, 95% CI 0.65–0.89) variant carriers in the retrospective analysis, with consistent, though not statistically significant, findings from the prospective analysis. In the prospective analysis, higher BMI and adult weight gain were associated with higher postmenopausal breast cancer risk forBRCA1carriers (HR 1.20 per 5 kg/m2, 95% CI 1.02–1.42; and HR 1.10 per 5 kg weight gain, 95% CI 1.01–1.19, respectively).ConclusionAnthropometric measures are associated with breast cancer risk forBRCA1andBRCA2variant carriers, with relative risk estimates that are generally consistent with those for women from the general population.
Development and validation of an eco-friendly HPLC–UV method for determination of atorvastatin and vitamin D3 in pure form and pharmaceutical formulation
Authors: Maged Khaled , El-Henawee Magda M. , Abd El-Hay Soad S. 
Published: June 2023

AbstractStatin-associated muscle symptoms are considered as obvious adverse effects of prolonged statin therapy such as myopathy, myalgia, and rhabdomyolysis. These side effects are associated with vitamin D3 deficiency and can be adjusted by amendment of serum vitamin D3 level. Green chemistry aims to decrease the harmful effects of analytical procedures. Here we have developed a green and eco-friendly HPLC method for the determination of atorvastatin calcium and vitamin D3. The two drugs were separated in less than 10 min on Symmetry column C18 (100 × 4.6 mm, 3.5 µm) using a mixture consisting of 0.1% ortho-phosphoric acid (OPA) (pH = 2.16) and ethanol as the mobile phase in gradient manner. We have used Green Analytical Procedure Index (GAPI) tools and the Analytical GREEnness Metric Approach (AGREE) for assessment of the greenness of our proposed method. The method proved linearity over concentration ranges of (5–40) and (1–8) µg/ml with low limit of detection of 0.475 and 0.041 µg/ml for atorvastatin calcium and vitamin D3 respectively. The method was successfully validated in accordance with ICH instructions and utilized for determination of the drugs of interest either in pure form or in their pharmaceuticals.
Fluralaner (Bravecto®) treatment kills Aedes aegypti after feeding on Dirofilaria immitis-infected dogs
Authors: Duncan Kathryn , Barrett Anne W. , Little Susan E. , Sundstrom Kellee D. , Guerino Frank 
Published: June 2023

Abstract
Background
Transmission of canine heartworm (Dirofilaria immitis) from infected to naïve dogs is dependent on successful mosquito feeding and survival.

Methods
To determine whether treating heartworm-infected dogs with fluralaner (Bravecto®) limits the survival of infected mosquitoes, and potentially the transmission of D. immitis, we allowed female mosquitoes to feed on microfilaremic dogs and evaluated mosquito survival and infection with D. immitis. Eight dogs were experimentally infected with D. immitis. On day 0 (~ 11 months post-infection), four microfilaremic dogs were treated with fluralaner according to label directions while the other four were non-treated controls. Mosquitoes (Aedes aegypti Liverpool) were allowed to feed on each dog on days −7, 2, 30, 56, and 84. Fed mosquitoes were collected, and the number of live mosquitoes determined at 6 h, 24 h, 48 h, and 72 h post-feeding. Surviving mosquitoes held for 2 weeks were dissected to confirm third-stage D. immitis larvae; PCR (12S rRNA gene) was performed post-dissection to identify D. immitis in mosquitoes.

Results
Prior to treatment, 98.4%, 85.1%, 60.7%, and 40.3% of mosquitoes fed on microfilaremic dogs were alive at 6 h, 24 h, 48 h, and 72 h post-feeding, respectively. Similarly, mosquitoes fed on microfilaremic, non-treated dogs were alive 6 h post-feeding (98.5–100%) throughout the study. In contrast, mosquitoes fed on fluralaner-treated dogs 2 days after treatment were dead or severely moribund by 6 h post-feeding. At 30 and 56 days post-treatment, > 99% of mosquitoes fed on treated dogs were dead by 24 h. At 84 days post-treatment, 98.4% of mosquitoes fed on treated dogs were dead by 24 h. Before treatment, third-stage larvae of D. immitis were recovered from 15.5% of Ae. aegypti 2 weeks after feeding, and 72.4% were positive for D. immitis by PCR. Similarly, 17.7% of mosquitoes fed on non-treated dogs had D. immitis third-stage larvae 2 weeks after feeding, and 88.2% were positive by PCR. Five mosquitoes fed on fluralaner-treated dogs survived 2 weeks post-feeding, and 4/5 were from day 84. None had third-stage larvae at dissection, and all were PCR-negative.

Conclusion
The data indicate that fluralaner treatment of dogs kills mosquitoes and thus would be expected to reduce transmission of heartworm in the surrounding community.

Graphical Abstract
Factors predicting mathematics achievement in PISA: a systematic review
Authors: Wang Xiaofang Sarah , Perry Laura B. , Malpique Anabela , Ide Tobias 
Published: June 2023

AbstractThe Programme for International Student Assessment (PISA) has become the world’s largest comparative assessment of academic achievement. While hundreds of studies have examined the factors predicting student achievement in PISA, a comprehensive overview of the main predictors has yet to be completed. To address this gap, we conducted a systematic literature review of factors predicting mathematics performance in PISA. Guided by Bronfenbrenner’s ecological model of human development, we synthesized the findings of 156 peer reviewed articles. The analysis identified 135 factors that fall into five broad categories: individual student, household context, school community, education systems and macro society. The analysis uncovered seven factors that are consistently associated with math achievement in PISA. Student grade level and overall family SES (socio-economic status) are consistently positively associated with math achievement while five factors are consistently negatively associated with math achievement: student absenteeism and lack of punctuality, school repeating and dropout rate, school prevalence of students’ misbehavior, shortage of teachers and general staff, and student-centered instruction. Fourteen factors tend to be positively or negatively associated with math achievement. The explanatory power of many other factors, however, remain mixed. Explanations for this result include methodological differences, complex interactions across variables, and underlying patterns related to national-cultural context or other meso or macro-level variables. Implications for policy and research are discussed.
Mechanochemical-assisted extraction and enzymatic hydrolysis of calcium phytate from defatted rice bran
Authors: Zheng Lei , Zhu XingYi , Wang Jialu , Su Weike 
Published: June 2023

Abstract
Background
Mechanochemical-assisted extraction (MCAE) and mechanochemical-assisted enzyme catalysis (MCAEC) methods were developed for extraction and enzymatic hydrolysis of calcium phytate from defatted rice bran, respectively.

Results
The MCAE conditions were optimized by response surface methodology: 10% sodium carbonate as the solid reagent, 33 min grinding time, 18:1 ball–material ratio, 330 rpm ball milling speed, and pH 5.5 extraction. Under these conditions, the yield of calcium phytate was 97.0 mg/g. The MCAEC conditions were optimized as follows: 2% enzyme addition, MM400 milling time 36 min, PM400 milling speed 262 rpm, MM400 milling frequency 27 Hz, and the addition of pH 5.5 sodium acetate buffer 3 mL. Under these conditions, the hydrolysis rate of calcium phytate was 39.7%.

Conclusion
Compared with traditional extraction and enzymatic hydrolysis methods, MCAE and MCAEC could obtain higher yields and hydrolysis rates, respectively.

Graphical Abstract
Child injuries in Lebanon: assessing mothers’ injury prevention knowledge attitude and practices
Authors: Al-Hajj Samar , El Haj Rawan , Chaaya Monique , Sharara-Chami Rana , Mehmood Amber 
Published: June 2023

Abstract
Background
Childhood injury is a neglected public health problem with a sizeable burden on children’s well-being and their families. This study aims to describe the pattern and types of childhood injuries and to determine the level of mothers’ Knowledge, Attitude, and Practices (KAP) towards childhood injury prevention in Lebanon. The study further examines the association between childhood injury occurrence and mothers’ supervision.

Methods
This cross-sectional study recruited mothers of children aged up to 10 years from multiple sites (i.e., a medical center, a private clinic, a healthcare facility, and a refugee camp clinic). Data were collected on mothers’ KAP toward childhood injuries using self-administrated questionnaires. A summation score for KAP correct answers was calculated and descriptive and statistical analyses were performed to measure the association between the outcomes.

Results
A total of 264 mothers were surveyed and injury data were collected on their 464 children. The prevalence of childhood injury was 20% in the past 12 months, mostly sustained by males (53.8%) and children aged 5–10 years (38.7%). The most common type of injury was fall (48.4%), followed by burns (%7.5), and sports injuries (7.5%). Hospitalized children were more likely to be males and older than 5 years (p < 0.001). More than one-third of the mothers demonstrated poor knowledge, while the majority showed poor practice (54.4%), and fair attitude (45.6%) towards child injury prevention. Children of working mothers have three times higher odds of sustaining injuries (OR: 2.95, 95% CI: 1.60;5.47) compared to those of non-working mothers, accounting for possible confounders (p = 0.001).

Conclusion
Childhood injuries represent a major health problem in Lebanon. Findings from this study showed that mothers are less knowledgeable and unprepared to prevent their children from getting injured. Educational programs are much needed to address the gap in the mothers' KAP toward child injury prevention. Further studies are recommended to understand the cultural context and examine its key determinants to identify effective strategies and develop tailored interventions for preventing childhood injuries.
Using preoperative ultrasound vascularity characteristics to estimate medullary thyroid cancer
Authors: Gao Luying , Ma Liyuan , Li Xiaoyi , Liu Chunhao , Li Naishi , Lian Xiaolan , Xia Weibo , Liu Ruifeng , Shi Xinlong , Ji Jiang , Pan Aonan , Xia Yu , Jiang Yuxin 
Published: June 2023

Abstract
Background
The early diagnosis of medullary thyroid carcinoma (MTC) is still a challenge in clinical practice. Based on ultrasound features, many MTC cases without suspicious characteristics are not categorized as high risk for malignancy. This study was designed to comprehensively investigate the ultrasonic features of MTC on ultrasound and help identify thyroid nodules with a high risk of MTC.

Methods
Between 2017 and 2023, we retrospectively reviewed 116 consecutive thyroid nodules with a histologic diagnosis of MTC who had undergone preoperative ultrasound examination. According to the ultrasonic criteria for risk classification, nodules were classified as “ultrasound-high suspicious” (h-MTC) and “ultrasound-low suspicious” (l-MTC). Using the same database, a tumour size- and risk evaluation-matched control group comprising 62 lesions was randomly selected to compare the vascularity features of l-MTC disease.

Results
We identified 85 h-MTC nodules (73.3%) and 31 l-MTC nodules (26.7%). For patients with l-MTC disease, 22/31 (71.0%) of the lesions were followed up for a period before fine needle aspiration (FNA) or surgery. We observed more penetrating branching vascularity in the l-MTC group than in the benign nodule group (23/31, 74.2% vs. 5/59, 4.8%, P < 0.001). We also showed that more CHAMMAS IV patterns (central blood flow greater than perinodular flow) (87.1% vs. 32.3%, P < 0.001)) and CHEN IV patterns (penetrating vascularity) (100% vs. 25.8%, P < 0.001) were found in l-MTC than benign nodules.

Conclusions
Vascularity features can help differentiate l-MTC from benign nodules; moreover, we report a novel sonographic vascularity pattern of l-MTC disease, penetrating branching vascularity. The utilization of vascularity features will help to identify MTC among nodules with low-intermediate suspicion by ultrasound risk classification to ensure appropriate clinical management.
Enhancement of bone regeneration by coadministration of angiogenic and osteogenic factors using messenger RNA
Authors: Zhang Maorui , Fukushima Yuta , Nozaki Kosuke , Nakanishi Hideyuki , Deng Jia , Wakabayashi Noriyuki , Itaka Keiji 
Published: June 2023

Abstract
Background
Bone defects remain a challenge today. In addition to osteogenic activation, the crucial role of angiogenesis has also gained attention. In particular, vascular endothelial growth factor (VEGF) is likely to play a significant role in bone regeneration, not only to restore blood supply but also to be directly involved in the osteogenic differentiation of mesenchymal stem cells. In this study, to produce additive angiogenic-osteogenic effects in the process of bone regeneration, VEGF and Runt-related transcription factor 2 (Runx2), an essential transcription factor for osteogenic differentiation, were coadministered with messenger RNAs (mRNAs) to bone defects in the rat mandible.

Methods
The mRNAs encoding VEGF or Runx2 were prepared via in vitro transcription (IVT). Osteogenic differentiation after mRNA transfection was evaluated using primary osteoblast-like cells, followed by an evaluation of the gene expression levels of osteogenic markers. The mRNAs were then administered to a bone defect prepared in the rat mandible using our original cationic polymer-based carrier, the polyplex nanomicelle. The bone regeneration was evaluated by micro-computerized tomography (μCT) imaging, and histologic analyses.

Results
Osteogenic markers such as osteocalcin (Ocn) and osteopontin (Opn) were significantly upregulated after mRNA transfection. VEGF mRNA was revealed to have a distinct osteoblastic function similar to that of Runx2 mRNA, and the combined use of the two mRNAs resulted in further upregulation of the markers. After in vivo administration into the bone defect, the two mRNAs induced significant enhancement of bone regeneration with increased bone mineralization. Histological analyses using antibodies against the Cluster of Differentiation 31 protein (CD31), alkaline phosphatase (ALP), or OCN revealed that the mRNAs induced the upregulation of osteogenic markers in the defect, together with increased vessel formation, leading to rapid bone formation.

Conclusions
These results demonstrate the feasibility of using mRNA medicines to introduce various therapeutic factors, including transcription factors, into target sites. This study provides valuable information for the development of mRNA therapeutics for tissue engineering.
Propylene glycol and Kolliphor as solvents for systemic delivery of cannabinoids via intraperitoneal and subcutaneous routes in preclinical studies: a comparative technical note
Authors: Momenzadeh Kaveh , Yeritsyan Diana , Kheir Nadim , Nazarian Rosalyn M. , Nazarian Ara 
Published: June 2023

Abstract

Background
Substance administration to laboratory animals necessitates careful consideration and planning in order to enhance agent distribution while reducing any harmful effects from the technique. There are numerous methods for administering cannabinoids; however, several parameters must be considered, including delivery frequency, volume of administration, vehicle, and the level of competence required for staff to use these routes properly. There is a scarcity of information about the appropriate delivery method for cannabinoids in animal research, particularly those that need the least amount of animal manipulation during the course of the investigation. This study aims to assess the feasibility and potential side effects of intraperitoneal and subcutaneous injection of CBD and THC using propylene glycol or Kolliphor in animal models. By evaluating the ease of use and histopathological side effects of these solvents, this study intends to help researchers better understand an accessible long-term delivery route of administration in animal experiments while minimizing the potential confounding effects of the delivery method on the animal.

Methods
Intraperitoneal and subcutaneous methods of systemic cannabis administration were tested in rat models. Subcutaneous delivery via needle injection and continuous osmotic pump release were evaluated using propylene glycol or Kolliphor solvents. In addition, the use of a needle injection and a propylene glycol solvent for intraperitoneal (IP) administration was investigated. Skin histopathological changes were evaluated following a trial of subcutaneous injections of cannabinoids utilizing propylene glycol solvent.

Discussion
Although IP delivery of cannabinoids with propylene glycol as solvent is a viable method and is preferable to oral treatment in order to reduce gastrointestinal tract degradation, it has substantial feasibility limitations. We conclude that subcutaneous delivery utilizing osmotic pumps with Kolliphor as a solvent provides viable and consistent route of administration for long-term systemic cannabinoid delivery in the preclinical context.
The contentious relationship between artificial sweeteners and cardiovascular health
Authors: Singh Sandeep , Kohli Aditya , Trivedi Stuti , Kanagala Sai Gautham , Anamika F. N. U. , Garg Nikita , Patel Meet A. , Munjal Ripudaman Singh , Jain Rohit 
Published: June 2023

AbstractSweet has always been a fundamental human taste, and while glucose and other kinds of sugar are our primary energy sources, they can also have detrimental effects on health, including weight gain, obesity, cardiovascular diseases, diabetes, and other metabolic diseases. Artificial sweeteners (AS), introduced as sugar substitutes, are a group of chemical compounds that attribute sweetness with almost zero calories and are considered safe for consumption by the Food and Drug Administration (FDA). Although they may help restrict the daily caloric intake of sugar to less than 10% of the daily caloric intake, there are still questions about the long-term safety of AS. A higher risk of hypertension, insulin resistance, high blood sugar, abdominal obesity, and dyslipidemia has been linked to AS. The effect of AS on the cardiovascular system is still unclear, and further research is required. This review examines the potential mechanism of how artificial sweeteners cause cardiovascular diseases.
Analysis of the geothermal formation mechanisms in the Gonghe Basin based on thermal parameter inversion
Authors: Bai Lige , Li Jing , Zeng Zhaofa , Wang Kun 
Published: June 2023

Gonghe Basin is the most critical distribution area of hot dry rock (HDR) geothermal resources in China. Geophysical results (resistivity, velocity, density, etc.) provide an indirect way to image the spatial distribution to understand its genesis, which has a multisolution problem. However, heat flow and thermal conductivity are the most direct and reliable evidence to reveal the distribution of geothermal resources. The conventional thermal conductivity and heat flow obtained based on geologic surveys and rock samples are highly accurate, but it is challenging to characterize the regional distribution characteristics. In this paper, we develop the heat transfer adaptive finite-element equation parameter inversion workflow to invert the thermal parameters and analyze the geothermal formation mechanisms in the Gonghe Basin. First, the synthetic model test verifies that our inversion process has reliable accuracy and strong antinoise ability. For the Gonghe Basin HDR geothermal example, we use high-precision aeromagnetic data to estimate the Curie depth using the improved Parker-Oldenburg method. Then, the regional conductivity thermal and temperature field can be established according to the empirical formula as the initial input model for the inversion procedure. In addition, we collect the temperature data of six HDR geothermal wells across the Gonghe-Guide Basin as a priori constraint information. Combined with the inverted thermal parameters (thermal conductivity, geothermal gradient, and heat flow), geologic structure, and geophysical result, we infer that the high thermal conductivity layer at a depth of 5–10 km provides the main deep heat source channel and the shallow low thermal conductivity fine sandstone layer of Neogene provides good caprock protection for forming a high-temperature geothermal reservoir.