bluuebunny/crossref_metadata_2025_split huggingface dataset:
An Arm-to-Disarm Strategy to Overcome Phenotypic AMR inMycobacterium tuberculosis
Authors: Kumar T. Anand , Birua Shalini , Chandra M. Sharath , Mukherjee Piyali , Singh Samsher , Kaul Grace , Akhir Abdul , Chopra Sidharth , Hirschi Jennifer , Singh Amit , Chakrapani Harinath 
Published: March 2023

ABSTRACTMost front-line tuberculosis drugs are ineffective against hypoxic non-replicating drug-tolerantMycobacterium tuberculosis(Mtb) contributing to phenotypic antimicrobial resistance (AMR). This is largely due to the poor permeability in the thick and waxy cell wall of persister cells, leading to diminished drug accumulation and reduced drug-target engagement. Here, using an “arm-to-disarm” prodrug approach, we demonstrate that non-replicatingMtbpersisters can be sensitized to Moxifloxacin (MXF), a front-line TB drug. We design and develop a series of nitroheteroaryl MXF prodrugs that are substrates for bacterial nitroreductases (NTR), a class of enzymes that are over-expressed in hypoxicMtb. Enzymatic activation involves electron-transfer to the nitroheteroaryl compound followed by protonation via water that contributes to the rapid cleavage rate of the protective group by NTR to produce the active drug. Phenotypic and genotypic data are fully consistent with MXF-driven lethality of the prodrug inMtbwith the protective group being a relatively innocuous bystander. The prodrug increased intracellular concentrations of MXF than MXF alone and is more lethal than MXF in non-replicating persisters. Hence, arming drugs to improve permeability, accumulation and drug-target engagement is a new therapeutic paradigm to disarm phenotypic AMR.
Phosphoregulation of the Rad51 auxiliary factor Swi5-Sfr1
Authors: Liang Pengtao , Lister Katie , Yates Luke , Argunhan Bilge , Zhang Xiaodong 
Published: March 2023

ABSTRACTHomologous recombination (HR) is a major pathway for the repair of DNA double-strand breaks, the most severe form of DNA damage. The Rad51 protein is central to HR, but multiple auxiliary factors regulate its activity. The heterodimeric Swi5-Sfr1 complex is one such factor. It was previously shown that two sites within the intrinsically disordered domain of Sfr1 are critical for the interaction with Rad51. Here, we show that phosphorylation of five residues within this domain regulates the interaction of Swi5-Sfr1 with Rad51. Biochemical reconstitutions demonstrated that a phosphomimetic mutant version of Swi5-Sfr1 is defective in both the physical and functional interaction with Rad51. This translated to a defect in DNA repair, with the phosphomimetic mutant yeast strain phenocopying the previously established interaction mutant. Interestingly, a strain in which Sfr1 phosphorylation was blocked also displayed sensitivity to DNA damage. Taken together, we propose that controlled phosphorylation of Sfr1 is important for the role of Swi5-Sfr1 in promoting Rad51-dependent DNA repair.
Adverse outcomes in SARS-CoV-2 infected pregnant mice are gestational age-dependent and resolve with antiviral treatment
Authors: Creisher Patrick S. , Perry Jamie L. , Zhong Weizhi , Lei Jun , Mulka Kathleen R , Ryan Hurley , Zhou Ruifeng , Akin Elgin H. , Liu Anguo , Mitzner Wayne , Burd Irina , Pekosz Andrew , Klein Sabra L. 
Published: March 2023

AbstractSARS-CoV-2 infection during pregnancy is associated with severe COVID-19 and adverse fetal outcomes, but the underlying mechanisms remain poorly understood. Moreover, clinical studies assessing therapeutics against SARS-CoV-2 in pregnancy are limited. To address these gaps, we developed a mouse model of SARS-CoV-2 infection during pregnancy. Outbred CD1 mice were infected at embryonic day (E) 6, E10, or E16 with a mouse adapted SARS-CoV-2 (maSCV2) virus. Outcomes were gestational age-dependent, with greater morbidity, reduced anti-viral immunity, greater viral titers, and more adverse fetal outcomes occurring with infection at E16 (3rdtrimester-equivalent) than with infection at either E6 (1sttrimester-equivalent) or E10 (2ndtrimester-equivalent). To assess the efficacy of ritonavir-boosted nirmatrelvir (recommended for pregnant individuals with COVID-19), we treated E16-infected dams with mouse equivalent doses of nirmatrelvir and ritonavir. Treatment reduced pulmonary viral titers, decreased maternal morbidity, and prevented adverse offspring outcomes. Our results highlight that severe COVID-19 during pregnancy and adverse fetal outcomes are associated with heightened virus replication in maternal lungs. Ritonavir-boosted nirmatrelvir mitigated adverse maternal and fetal outcomes of SARS-CoV-2 infection. These findings prompt the need for further consideration of pregnancy in preclinical and clinical studies of therapeutics against viral infections.
Single-cell mRNA-regulation analysis reveals cell type-specific mechanisms of type 2 diabetes
Authors: Martínez-López J.A. , Lindqvist A. , Lopez-Pascual A. , Chen P. , Shcherbina L. , Chriett S. , Skene N.G. , Prasad R.B. , Lancien M. , Johnson P.F. , Eliasson P. , Louvet C. , Muñoz-Manchado A.B. , Sandberg R. , Hjerling-Leffler J. , Wierup N. 
Published: March 2023

ABSTRACTPerturbed secretion of insulin and other pancreatic islet hormones is the main cause of type 2 diabetes (T2D). The islets harbor five cell types that are potentially altered differently by T2D. Whole-islet transcriptomics and single-cell RNA-sequencing (scRNAseq) studies have revealed differentially expressed genes without reaching consensus. Here, we demonstrate that unprecedented insights into disease mechanisms can be obtained by network-based analysis of scRNAseq data. We developed differential gene coordination network analysis (dGCNA) and analyzed islet scRNAseq data from 16 T2D and 16 non-T2D individuals. dGCNA revealed T2D-induced cell type-specific networks of dysregulated genes with remarkable ontological specificity, thus allowing for a comprehensive and unbiased functional classification of genes involved in T2D. In beta cells eleven networks of genes were detected, revealing that mitochondrial electron transport chain, glycolysis, cytoskeleton organization, cell proliferation, unfolded protein response and three networks of beta cell transcription factors are perturbed, whereas exocytosis, lysosomal regulation and insulin translation programs are instead enhanced in T2D. Furthermore, we validated the ability of dGCNA to reveal disease mechanisms and predict the functional context of genes by showing thatTMEM176A/Bregulates the beta cell cytoskeleton and thatCEPBGis a key regulator of the unfolded protein response. In addition, comparing beta- and alpha and cells, we found substantial differences, reproduced across independent datasets, confirming cell type-specific alterations in T2D. We conclude that analysis of networks of differentially coordinated genes provides outstanding insight into cell type-specific gene function and T2D pathophysiology.
NPOs and private governance forms for football clubs: towards a blended model
Authors: Agostino Deborah , Thomasson Anna 
Published: March 2023

PurposeThis study explores the relationship between governance model – private organisations vs non-profit organisations (NPOs) – and performance in football clubs.Design/methodology/approachThe study is a comparative case study of two football clubs with different governance models: Malmö FF, which is an NPO, and Bologna FC, which is a privately owned club.FindingsThe results show that both football clubs focus equally on financial and non-financial performance, and in practice, both clubs use a blend of private and NPO governance models. While supporting efforts towards financial results, blending the models appears to support football clubs' management of the tension between financial and non-financial performance and the expectation that they will contribute to local development. Thus, using a blend of the two models is not only accepted but expected.Research limitations/implicationsThis study is a comparative case study of two football clubs. This study furthers our understanding of how football clubs manage the tension between financial and non-financial performance expectations. This is particularly of interest in light of the increasing professionalisation of sports, especially football, and how this might jeopardise the contributions that sport clubs make to the local community.Originality/valueBy exploring the relationship between governance model and performance, this study shows that, contrary to expectations, privately owned football clubs focus as much on non-financial performance as clubs governed as NPOs. This study contributes to the existing literature by showing how clubs use a mixture of elements from governance models to manage the tension between financial and non-financial performance that has emerged in the wake of the increasing professionalisation of football.
Enhanced WPT structures via EC-SRR-based metasurfaces
Authors: Karatzidis Dimitrios I. , Zygiridis Theodoros T. , Kantartzis Nikolaos V. 
Published: March 2023

Purpose
The purpose of this paper is to present a family of robust metasurface-oriented wireless power transfer systems with improved efficiency and size compactness. The effect of geometric and structural features on the overall efficiency and miniaturisation is elaborately studied, while the presence of substrate losses is, also, considered. Moreover, to further enhance the performance, possible means for reducing the operating frequency, without comprising the unit-cell size, are proposed.

Design/methodology/approach
The key element of the design technique is the edge-coupled split-ring resonators patterned in various metasurface configurations and optimally placed to increase the total efficiency. To this goal, a rigorous three-dimensional algorithm, launching a new high-order prism macroelement, is developed in this paper for the fast evaluation of the required quantities. The featured scheme can host diverse approximation orders, while it is drastically more economical than existing methods. Hence, the demanding wireless power transfer systems are precisely modelled via reduced degrees of freedom, without the need to conduct large-scale simulations.

Findings
Numerical results, compared with measured data from fabricated prototypes, validate the design methodology and prove its competence to provide enhanced metasurface wireless power transfer systems. An assortment of optimized 3 x 3 and 5 x 5 metamaterial setups is investigated, and interesting deductions, regarding the impact of the inter-element gaps, the distance between the transmitting and receiving components and the substrate losses, are derived. Also, the proposed vector macroelement technique overwhelms typical implementations in terms of computational burden, particularly when combined with the relevant commercial software packages.

Originality/value
Systematic design of advanced real-world wireless power transfer structures through optimally selected metasurfaces with fully controllable electromagnetic properties is presented. The analysis is performed by means of a rapid prism macroelement methodology, which leads to very confined meshes, accurate results and significantly reduced overhead. The selected metamaterial resonators are found to be very flexible and reconfigurable, even in the case of large substrate conductivity losses, whereas their contribution to the system’s total efficiency is decisive.
Predicting song genre with deep learning
Authors: Marijić Antonijo , Bagić Babac Marina 
Published: March 2023

Purpose
Genre classification of songs based on lyrics is a challenging task even for humans, however, state-of-the-art natural language processing has recently offered advanced solutions to this task. The purpose of this study is to advance the understanding and application of natural language processing and deep learning in the domain of music genre classification, while also contributing to the broader themes of global knowledge and communication, and sustainable preservation of cultural heritage.

Design/methodology/approach
The main contribution of this study is the development and evaluation of various machine and deep learning models for song genre classification. Additionally, we investigated the effect of different word embeddings, including Global Vectors for Word Representation (GloVe) and Word2Vec, on the classification performance. The tested models range from benchmarks such as logistic regression, support vector machine and random forest, to more complex neural network architectures and transformer-based models, such as recurrent neural network, long short-term memory, bidirectional long short-term memory and bidirectional encoder representations from transformers (BERT).

Findings
The authors conducted experiments on both English and multilingual data sets for genre classification. The results show that the BERT model achieved the best accuracy on the English data set, whereas cross-lingual language model pretraining based on RoBERTa (XLM-RoBERTa) performed the best on the multilingual data set. This study found that songs in the metal genre were the most accurately labeled, as their text style and topics were the most distinct from other genres. On the contrary, songs from the pop and rock genres were more challenging to differentiate. This study also compared the impact of different word embeddings on the classification task and found that models with GloVe word embeddings outperformed Word2Vec and the learning embedding layer.

Originality/value
This study presents the implementation, testing and comparison of various machine and deep learning models for genre classification. The results demonstrate that transformer models, including BERT, robustly optimized BERT pretraining approach, distilled bidirectional encoder representations from transformers, bidirectional and auto-regressive transformers and XLM-RoBERTa, outperformed other models.
Study of flow field and vibration characteristics of supersonic centrifugal impellers based on fluid-structure interaction
Authors: Li Huanjun , Zhang Yimin 
Published: March 2023

Purpose
There are three purposes in this paper: to verify the importance of bi-directional fluid-structure interaction algorithm for centrifugal impeller designs; to study the relationship between the flow inside the impeller and the vibration of the blade; study the influence of material properties on flow field and vibration of centrifugal blades.

Design/methodology/approach
First, a bi-directional fluid-structure coupling finite element numerical model of the supersonic semi-open centrifugal impeller is established based on the Workbench platform. Then, the calculation results of impeller polytropic efficiency and stage total pressure ratio are compared with the experimental results from the available literature. Finally, the flow field and vibrational characteristics of 17-4PH (PHB), aluminum alloy (AAL) and carbon fiber-reinforced plastic (CFP) blades are compared under different operating conditions.

Findings
The results show that the flow fields performance and blade vibration influence each other. The flow fields performance and vibration resistance of CFP blades are higher than those of 17-4PH (PHB) and aluminum alloy (AAL) blades. At the design speed, compared with the PHB blades and AAL blades, the CFP blades deformation is reduced by 34.5% and 9%, the stress is reduced by 69.6% and 20% and the impeller pressure ratio is increased by 0.8% and 0.14%, respectively.

Originality/value
The importance of fluid-structure interaction to the aerodynamic and structural design of centrifugal impeller is revealed, and the superiority over composite materials in the application of centrifugal impeller is verified.
Board characteristics and corporate cash holding: evidence from the UK, France and Germany
Authors: Ezeani Ernest , Salem Rami Ibrahim A. , Usman Muhammad , Kwabi Frank , Bilal  
Published: March 2023

Purpose
Prior studies suggest that corporate cash holding will reflect firms' corporate governance (CG) environment. Consistent with this prediction, this study aims to examine the impact of board characteristics on firms' cash holding in the UK, France and Germany.

Design/methodology/approach
Using 2,805 firm-year observations between 2009 and 2019, the authors examine the relationship between board characteristics and corporate cash holding. The authors used two measures of cash holdings as our dependent variables. As independent variables, the authors used CG characteristics relevant to effective board monitoring such as board meetings, outside directors, board size and board gender diversity.

Findings
The authors find that board characteristics influence firms' cash holdings of firms in the UK, France and Germany. However, this study documents evidence of varying impacts of board monitoring on the cash holding of the UK when compared to German and French firms, the countries that are classifiable as bank-based economies. The result of this study is robust to alternative cash-holding measures and endogeneity.

Practical implications
This study provides evidence supporting the board's impact in mitigating agency conflict in shareholder- and stakeholder-oriented CG environments.

Originality/value
This study contributes to previous works on firms’ financial orientation by showing that the impact of board characteristics on corporate cash holdings varies between bank- and market-based economies.
The relationship between income smoothing and the cost of debt: evidence from the United Kingdom and Nigeria
Authors: Aboud Ahmed , Haruna Baba , Diab Ahmed 
Published: March 2023

Purpose
This paper aims to examine the association between income smoothing and the cost of debt in two different countries, namely, the UK and Nigeria.

Design/methodology/approach
The authors used a sample from listed firms in the UK and Nigeria during 2000–2019. The study hypotheses are examined by implementing quantitative methods, including panel regression analysis, cross-sectional regression analysis and parametric independent samples t-test.

Findings
The results reveal that Nigerian companies have a substantially higher cost of debt and are more active in using income-smoothing practices. However, the relationship between income smoothing and the cost of debt is not found to be statistically significant in both countries. Besides, the results of this study show that financial leverage, profitability, company size and asset turnover are significantly associated with the cost of debt.

Originality/value
The study contributes to the existing literature by providing new insights concerning the contrast between developed and developing countries in financial and reporting issues.
The interaction effect of trust and contract on dispute negotiation strategy: evidence from the Chinese construction industry
Authors: FU Yafan , Luo Yangyang 
Published: March 2023

PurposeThis paper aims to investigate how and when different dimensions of trust and contracts interact to influence the development to negotiation strategies. Specifically, it explores how different dimensions of trust and contracts are combined to influence dispute negotiation strategies when cooperation parties have or do not have expectations of continuity.Design/methodology/approachThis paper theoretically identified and empirically examined the interaction effect of trust and contract on dispute negotiation strategies in contractor–subcontractor relationships, by developing a conceptual framework and conducting a questionnaire survey comprising more than 300 disputes in the Chinese construction industry. Hierarchical regression analysis was mainly used to test the hypotheses.FindingsThis paper finds that contractual control may weaken the effect of goodwill trust in fostering interest-based strategies in the presence of expectations of continuity, while it may strengthen the effect in the absence of expectations of continuity. Contractual coordination negatively moderates the relationship between goodwill trust and interest-based strategies only when parties have little expectations of continuity. Moreover, contractual control enhances the effectiveness of competence trust on fostering interest-based strategies.Practical implicationsThis paper provides insights for practitioners to wisely use different governance mechanisms to manage negotiation strategies and generate desired outcomes of dispute resolution.Originality/valueThis paper provides a nuanced understanding of how the two types of governance mechanisms interact, by considering trust and contract as multi-dimensional constructs. It explicit the boundary conditions of both the substitute and complementary relationship between them.
A model of financial support for the poor to access health services in Iran: Delphi technique
Authors: Etemadi Manal , Ashtarian Kioomars , Ganji Nader 
Published: March 2023

PurposeReducing inequity in health between the poor and the rich is one of the challenges of the Iranian health sector. Access to health services in Iran is lower in the lowest-income quarter, and the rich use health services more. The purpose of this study is to provide a comprehensive framework for enabling financial access by the poor to health services in Iran.Design/methodology/approachPolicy options were validated and approved by experts and specialists in two stages using the Delphi technique. The sample was consisted of 22 well-known experts on the subject who were selected based on purposive sampling. To evaluate the reliability of the questionnaire, a pilot study was conducted with five participants. Dimensional validity of the policy model, which was agreed upon by more than 75% of the participants was acceptable.FindingsThe main aspects of the model were divided into five categories: identifying the poor, policymaking to prevent the aggravation of health poverty, providing targeted funding, highlighting the importance of coherent regulation and ensuring financial accessibility to health services for the poor. This model could align the activities of all stakeholders in the form of a network and considers its prerequisites.Originality/valuePrevention of dire financial consequences in the case of referral to follow up the treatment alongside exemption and financial protection policies through the networking activities of organizations involved in this field is a crucial step in securing financial support for the poor. Although the researchers included a wide range of policymakers in the Delphi study to gather all perspectives about options for financially support the poor, there may be some potential neglected policy advices.