bluuebunny/crossref_metadata_2025_split huggingface dataset:
Development of a high-thermal-storage composite material embedded with PCM microcapsules and analysis of its unsteady heat transfer process
Authors: Wang Hui , Dang Jia , Zheng Miaozi , Yuan Yihui , Liu Tao , Wang Ning 
Published: May 2023

Abstract

Phase-change insulation materials can effectively extend the holding time of submarine oil and gas pipelines. Shape-stable phase-change composites have good application prospects owing to their chemical stability and leakage resistance. In this study, a microencapsulated phase-change material (MPCM)-embedded polyurethane (PU) composite (PU–MPCM) was prepared as an insulation material for subsea pipelines. The unsteady heat transfer process of the PU–MPCM composite was simulated using COMSOL Multiphysics, and an insulation experiment was conducted on this composite. PU–MPCM exhibited a high thermal storage performance and favorable shape stability. The relative effective enthalpy coefficients of PU–MPCM composites with different MPCM contents were greater than 80%. The maximum MPCM content in the PU–MPCM composite was 24 wt% (PU–MPCM24). The melting enthalpy of PU–MPCM24 reached 35.95 J/g, and its effective thermal conductivity was as low as 0.16 W/(m∙K). The holding time of PU–MPCM24 could be increased by 229.79% compared with that of pure PU. The PU–MPCM composite exhibited good mechanical properties and low water absorption, making it suitable for underwater environments. Owing to its low-cost preparation process and excellent thermal properties, PU–MPCM24 can be considered a potential insulation material for practical applications in subsea pipelines.
The benefits of predictive maintenance in manufacturing excellence: a case study to establish reliable methods for predicting failures.
Authors: Meddaoui Anwar , Hain Mustapha , Hachmoud Adil 
Published: May 2023

Abstract
In the course of manufacturing excellence, decision makers are consistently confronted with the task of making choices that will enhance and meet the plant's requirements. To this end, it is essential to maintain machines and equipment in a timely manner, which can prove to be one of the primary challenges. Predictive maintenance (PdM) technology can enable real-time maintenance, providing numerous benefits such as reduced downtime, lower costs, and improved production quality. This article tries to demonstrate efficient physical parameters used in PdM field. The paper presents a case study operated in industrial production process to compare between the most used algorithm in predicting equipment failures. Future research can improve prediction accuracy with other artificial intelligence tools.
A Novel Animal Model of Osteonecrosis of the Femoral Head Based on 3D Printing Technology
Authors: Li Yiyang , Zhang Jiewen , Zhao Yiwei , Duan Xudong , Cao Ruomu , Guan Huanshuai , Wu Zidong , Xing Fangze , Li Heng , Wang Kunzheng , Tian Run , Yang Pei 
Published: May 2023

Abstract
Background Osteonecrosis of the femoral head (ONFH) is a common orthopedic disease that is characterized by the interruption of blood supply to the femoral head. This leads to ischemia of the internal tissues, subchondral bone fractures, necrosis, and ultimately, the collapse of the weight-bearing portion of the femoral head, resulting in severe functional impairment, pain, and even disability of the hip joint. Currently, available animal models of ONFH are limited in their ability to accurately replicate the natural progression of the disease. Therefore, there is a need for the development of a new animal model that can better simulate the localized pressure on the human femoral head to facilitate research related to ONFH.Method In this study, we have developed a novel method for modeling ONFH that incorporates stress factors into the modeling process using 3D printing technology and principles of biomechanics. 36 animals were randomly assigned to six groups and received either a novel modeling technique or traditional hormone induction. Following an 8-week treatment period, Micro CT scans and histological evaluations were conducted to assess tissue outcomes.Results The new model effectively replicates the pathological features of ONFH, including femoral head collapse, with a large number of empty bone lacunae observed, cartilage defects, and subchondral bone fractures in the subchondral bone region. Furthermore, the new model shows the ability to simulate the progression of the disease, making it a valuable tool for research in this field.Conclusion In conclusion, our study provides evidence that the new ONFH model is a useful tool for simulating the disease and can contribute to the development of better treatment strategies for this debilitating condition. It holds great promise for advancing our understanding of the pathogenesis of ONFH and the potential therapeutic interventions for this challenging clinical problem.
Right Frontal HD-tDCS Reveals Causal Involvement of Time perception Networks in Temporal Processing of Concepts
Authors: Johari Karim , Tabari Fatemeh , Desai Rutvik H. 
Published: May 2023

Abstract
Evidence suggests that perceptual and action related features of concepts are grounded in the corresponding sensory-motor networks in the human brain. However, less is known about temporal features of event concepts (e.g., a lecture) and whether they are grounded in time perception networks. We examined this question by stimulating the right dorsolateral prefrontal cortex (rDLPFC) —a part of time perception network — using HD-tDCS and subsequently recording EEG while participants performed semantic and time perception tasks. Semantic tasks were composed of event nouns duration judgment (EDur), object nouns size judgment (OSize), valence judgement of event (EVal) and object (OVal) nouns. In the time perception task, participants judged the durations of pure tones. Results showed that stimulation accelerated responses for time perception task and decreased the magnitude of global field power (GFP) compared to sham stimulation. Semantic task results revealed that cathodal, but not sham, stimulation significantly decreased GFP for EDur relative to OSize and to EVal. These findings provide first causal evidence that temporal features of event words are grounded in the rDLPFC and in the temporal cognition network, and shed light on the conceptual processing of time.
Murine prostate cancer model: microenviroment macrophage populations and tumoral development vary within two C57BL/6 mice breeding nuclei from same substrain
Authors: Hernandez-Peralta Pablo , Chacón-Salinas Rommel , Gracia-Mora Isabel , Soldevila Gloria , Moreno-Rodríguez José , Cobos-Marín Laura 
Published: May 2023

Abstract
Cancer mice models are critical for immune-oncology research; they provide conditions to explore tumor immunoenviroment aiming knowledge advance and treatment development. Often, research groups breed their own mice colonies. To assess the effect of C57BL/6 mice breeding nuclei in prostate cancer development and intratumoral macrophage populations, a isotransplantation experiment was performed. C57BL/6J mice from two breeding nuclei (nA and nB) were employed for prostate adenocarcinoma TRAMP-C1 cells implantation, tumor growth period and intratumoral macrophage profile were measured. 54% of BL/6nB mice showed tumor implantation and growth period was 69 days, meanwhile BL/6nA implantation reached 100% and tumor growth occur in 28 days. No difference of total macrophage populations was observed between groups within several tumoral regions, significant higher M2 macrophage profile were founded in tumor microenviroment from both mice groups. Nevertheless, BL/6nB tumors showed around twice populations of M1 profile (11% -27%) than BL6nA (4% -15%) and less non-polarized macrophages. M2:M1 average ratio was 8:1 for A group and 4.2:1 for B. Our results demonstrate different tumor progression and intratumoral macrophage populations among mice from same substrain. Data obtained in this study shows the relevance of animal sources renewing for better control of murine cancer model variables.
Therapeutic role of 2-stearoxyphenethyl phosphocholine targeting microtubule dynamics and Wnt/β-catenin/EMT signaling in human colorectal cancer cells
Authors: Park Sang-Eun , Chung Kyung-Sook , Kim Soo-Yeon , Lee Jeong-Hun , Hassan Ahmed H.E. , Lee Yong Sup , Lee Jae Yeol , Lee Kyung -Tae 
Published: May 2023

AbstractThe inhibition of cell death, perturbation of microtubule dynamics, and acceleration of Wnt/β-catenin/epithelial-mesenchymal transition (EMT) signaling are fundamental processes in the progression and metastasis of colorectal cancer (CRC). To explore the role of 2-stearoxyphenethyl phosphocholine (stPEPC), an alkylphospholipid-based compound, in CRC, we conducted an MTT assay, cell cycle analysis, western blot analysis, immunoprecipitation, immunofluorescence staining, Annexin V/propidium iodide double staining, small interfering RNA gene silencing, a wound-healing assay, an invasion assay, and terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay in the human CRC cell lines HT29 and HCT116. stPEPC showed anti-proliferative properties and mitotic cell accumulation via upregulated phosphorylation of BUBR1 and an association between mitotic arrest deficiency 2 (MAD2) and cell division cycle protein 20 homolog (CDC20). These results suggest that activation of the mitotic checkpoint complex and tubulin polymerization occurred, resulting in mitotic catastrophe in HT29 and HCT116 cells. In addition, stPEPC attenuated cell migration and invasion by regulating proteins mediated by EMT, such as E-cadherin and occludin. stPEPC downregulated the protein expression of Wnt3a and phosphorylation of low-density lipoprotein receptor-related protein 6 (LRP6), glycogen synthase kinase 3β (GSK3β), and β-catenin as well as their target genes, including cMyc and cyclin D1, in CRC cells. stPEPC may be useful for developing new drugs to treat human CRC.
Demographic Factors Impacting Time to Diagnosis of Diffuse Large B-Cell Lymphoma
Authors: McAlarney Drury , Davitt Meghan , Shastri Aditi , Hosgood H. Dean 
Published: May 2023

Abstract
Purpose:
Diffuse large B-cell lymphoma (DLBCL) is the most common form of non-Hodgkin’s lymphoma, and patients are sometimes asymptomatic until the presentation of an oncological emergency, such as end organ complications, tumor lysis syndrome, or spinal cord compression. We aimed to investigate demographic and clinical factors associated with time from initial symptom onset until diagnosis of DLBCL.
Methods:
We performed a retrospective cohort study of 1076 patients with DLBCL diagnosed in the Montefiore Health System from 2005 to 2022. Logistic regression models, adjusted for race and preferred language, estimated the associations between demographic factors and increased time to diagnosis (TTD), defined as a time to diagnosis greater than the median for non-Hispanic White patients (58.5 days).
Results:
Within our cohort, 24.7% of patients were non-Hispanic White, 26.5% were non-Hispanic Black, and 39.86% were Hispanic. Median TTD was highest for Hispanic patients at 63 days (IQR 22–224) followed by non-Hispanic Whites at 58.5 (IQR 22–331) and 58 for non-Hispanic Black patients (IQR 17–237) (p = 0.03). Patients who had a preferred language of Spanish had increased odds of prolonged TTD in the multivariable model (adjusted OR = 2.06, 95% CI = 1.05–4.01, p = 0.03) compared to English speakers, as did patients with a preferred language of “other” (adjusted OR = 3.11, 95% CI = 1.01–9.59, p = 0.05.)
Conclusions:
Non-English-speaking patients experienced longer TTD of DLBCL compared to English speaking patients. Our results suggest that interventions increasing the availability of interpreter services, patient literature on preventative screenings in non-English languages, and increasing the number of healthcare workers with competency in common foreign languages, may potentially reduce TTD in non English speaking patients.
Association of adolescent postural tachycardia syndrome classifications with anxiety: a case control study
Authors: Mizutani Midori , Yoshida Seiji , Tanaka Hidetaka , Yamawake Ginroku , Kubo Atsuko , Ohta Yoshitaka , Kurooka Yusuke , Ashida Akira 
Published: May 2023

Abstract
Background
Postural tachycardia syndrome (POTS), a subset of orthostatic dysregulation, has been reported to be associated with anxiety. POTS can be classified into two forms based on the degree of tachycardia during orthostasis. Reportedly, POTS with decreased orthostatic heart rate increase is associated with suppressed cardiac parasympathetic activity and increased sympathetic activity in the supine position. In this study, the relationship between the two types of POTS and anxiety was evaluated in terms of autonomic function.
Methods
Fifty-two patients (23 males, aged 10–15 years) who were diagnosed with POTS at the Department of Pediatrics, Osaka Medical and Pharmaceutical University from 2019 to 2021, provided completed the State-Trait Anxiety Scale for Children (STAIC) questionnaire. They then completed a standing test and were accordingly classified into the two groups of POTS, the Su group, with tachycardia from the supine position and a low heart rate increase on standing, and the SI group, with a high heart rate increase during standing. Autonomic function was assessed by frequency analysis (MemCalc method) based on heart rate, blood pressure changes, heart rate, and blood pressure variability during the orthostatic test.
Results
Patients in the Su group had higher characteristic anxiety, lower cardiac parasympathetic activity (RR-HF) in the supine position, and greater variability in cardiac parasympathetic activity during orthostasis than those of patients in the SI group. The Su group had a greater decrease in cardiac index on standing than that of the SI group.
Conclusions
The Su group results may be partly attributed to chronically low venous return. We also found that patients in the Su group had low parasympathetic activity in the supine position, which may interact with the anxiety-prone characteristics of these patients. Therefore, it seems necessary to consider both physical and psychosomatic treatment approaches for patients with POTS.
Trial registration
The clinical trial registry of Osaka Medical and Pharmaceutical University, Clinical Trial Registration No. 2662-2. Registered 5 March 2019.
https://ompu.bvits.com/rinri/Common/print_approve.aspx?ID=3479
Comparison of Commercially Available Differentiation Media on Cell Morphology, Function, and Anti-Viral Responses in Conditionally Reprogrammed Human Bronchial Epithelial Cells
Authors: Awatade Nikhil T , Reid Andrew T , Nichol Kristy S , Budden Kurtis F , Veerati Punnam Chander , Pathinayake Prabuddha S , Grainge Christopher L , Hansbro Philip M , Wark Peter AB 
Published: May 2023

Abstract
Introduction:
Primary air liquid interface (ALI) cultures of bronchial epithelial cells are used extensively to model airway responses. A recent advance is the development of conditional reprogramming that enhances proliferative capability. Several different media and protocols are utilized, yeteven subtle differences may influence cellular responses. We compared the morphology and functional responses, including innate immune responses to rhinovirus infection in conditionally reprogrammed primary bronchial epithelial cells (pBECs) differentiated using two commonly used culture media.
Methods:
pBECs collected from healthy donors (n = 5) were CR using g-irradiated 3T3 fibroblasts and Rho Kinase inhibitor. CRpBECs were differentiated at ALI in either PneumaCult™ (PN-ALI) or Bronchial Epithelial Growth Medium (BEGM)-based differentiation media (BEBM:DMEM, 50:50, Lonza™) - (AB-ALI) for 28 days. Transepithelial electrical resistance (TEER), immunofluorescence, histology, cilia activity, ion channel function, and expression of cell markers were analyzed. Viral RNA was assessed by RT-qPCR and anti-viral proteins quantified by LEGENDplex™ following Rhinovirus-A1b (RVA1b) infection.
Results:
CRpBECs differentiated in PneumaCult™ were smaller and had a lower TEER and cilia beat frequency (CBF) compared to BEGM media. PneumaCult™ media cultures exhibited significantly increased FOXJ1 expression, more ciliated cells with a larger active area, increased intracellular mucins, and increased calcium-activated chloride channel current. However, there were no significant changes in viral RNA or host antiviral responses.
Conclusion:
There are distinct structural and functional differences in pBECs cultured in the two commonly used ALI differentiation media. Such factors need to be taken into consideration when designing CRpBECs ALI experiments for specific research questions.
Taxonomic structure of cyanoprokaryotes and microalgae in the steppe soil of Askania Nova
Authors: SOLONENKO ANATOLIY , BREN OLEKSANDR , BREN OKSANA 
Published: May 2023

Abstract
This study investigated the species composition and diversity of cyanoprokaryota and microalgae in the steppe soil of Askania Nova. There were identified a total of 135 species belonging to 6 phyla, 8 classes, 33 orders, 48 families, and 76 genera, with Chlorophyta, Cyanoprokaryota, and Xanthophyta being the most predominant in terms of species richness. The partial soil algoflora is diverse and stable over time due to the strict conservation regime in the reserve. The leading orders were Chlamydomonadales, Mischococcales, and Oscillatoriales, and the leading families were Chlamydomonadaceae, Pleurochloridaceae, and Leptolyngbyaceae. The most diverse genera were Chlamydomonas, Leptolyngbya, Phormidium, and Nostoc. There were also registered 39 species with the highest frequency of occurrence, including Hantzschia amphioxys, Klebsormidium flaccidum, and Pinnularia borealis. The prevalence of Chlamydomonadales representatives at all taxonomic levels suggests that anthropogenic factors may be impacting the area. Cluster and correspondence analyses showed conformity for certain associations in terms of similarity of species composition and species richness of cyanoprokaryota and microalgae.
Interactions between DC-SIGN and the envelope protein from Dengue and Zika viruses: a structural perspective based on molecular dynamics and MM/GBSA analyses
Authors: Menechino Bruno Stein Barbosa , Kato Rodrigo Bentes , Franz Helena Cristina Ferreira , Silva Pedro Eduardo Almeida , Corat Marcus , Neto Daniel Ferreira Lima 
Published: May 2023

Abstract
Zika virus (ZIKV) and Dengue Virus (DENV) share a lot of similarities, being both flaviviruses and neglected tropical diseases that cause a worldwide burden that is increasing with climate change. Studying the interactions of cell recptors and viruses is essential to further the knowledge and development of ways to prevent and control flaviviral diseases. An important interaction occurs between the envelopeof the two viruses and a protein on the surface of immune cells, called DC-SIGN. We describe this interaction in a structural comparison involving homology modeling of these proteins, guided docking based on deposited crystallography, molecular dynamics of the docked complexes and for the decomposition of interacting residues we employed an analysis of the surface area accessible by the Generalized Bourne method. The region of interest for the discussion is predominantly electropositive in the DENV envelope, but when comparing the same region in the ZIKV envelope, we can observe that there is a decrease in charges, not to the point of making the region electronegative as a whole, but enough for the surroundings of the interaction region to become neutral with electronegative trends. These results suggest better interaction of ZIKV with the DC-SIGN receptor, particularly in the CRD portion
Numerical multi-objective optimization of segmented and variable blank holder force trajectories in deep drawing based on DNN-GA-MCS strategy
Authors: Guo Feng , Jeong Hoyoung , Park Donghwi , Sung Booyong , Kim Naksoo 
Published: May 2023

Abstract
This research introduces a novel methodology for mitigating defects in sheet metal forming processes. The proposed approach employs a segmented and variable blank holder force (S-VBHF) trajectory, adjusting the blank holder force (BHF) during the forming cycle, enhancing formability, and reducing failure, wrinkling and springback defects. Optimal process parameters, including the S-VBHF, friction coefficient and drawbead restraining force (DBRF), were determined through a systematic methodology integrating deep neural network, genetic algorithm and Monte Carlo simulation (DNN-GA-MCS) techniques. The design constraint, defined as sheet failure during the forming process, was quantitatively evaluated using the forming limit diagram (FLD) to ensure rigorous assessment. The proposed methodology was validated through numerical simulations using a cylindrical cup provided by NUMISHEET 2011 (BM1) as test samples. The simulation results demonstrated a significant improvement in the formed sheet quality, characterized by reductions of 8.33%, 10.81% and 5.88% in failure, wrinkling and springback defects, respectively. These findings underscore the potential of the proposed approach in enhancing the quality of sheet metal forming processes and mitigating defects.
Noradrenaline depresses facial stimulation-evoked cerebellar MLI-PC synaptic transmission via α2-AR/PKA signaling cascade in vivo in mice
Authors: Wu Mao-Cheng , Chu Chun-Ping , Wang Jun-Ya , Weng Wen-Cai , Wang Ting-Qi , Liu Yue , Qiu De-Lai 
Published: May 2023

Abstract
The noradrenergic fibers of locus coeruleus (LC) together with mossy fibers and climbing fibers comprise the three types of cerebellar afferents, which modulate cerebellar neuronal circuit function via adrenergic receptors (ARs). We previously demonstrated that noradrenaline (NA) modulated synaptic transmission in mouse cerebellar cortex. In the present study, we investigated the effect of NA on the facial stimulation-evoked cerebellar molecular layer interneuron (MLI)-Purkinje cell (PC) synaptic transmission in urethane-anesthetized mice using in vivo cell-attached recording technique and pharmacological method. The MLI-PC synaptic transmission was induced by air-puff stimulation (duration: 60 ms) of ipsilateral whisker pad, which exhibited negative components (P1 and P2) accompanied with a pause of simple spike (SS). Cerebellar molecular layer application of NA (15 µM) decreased the amplitude and area under the curve (AUC) of P1, and the pause of SS, but increased the P2/P1 ratio. The NA-induced decrease in P1 amplitude was concentration-dependent, and the half inhibitory concentration (IC50) was 10.94 µM. The NA-induced depression of the facial stimulation-evoked MLI-PC GABAergic synaptic transmission was completely abolished by blockade of α-ARs or α2-AR, but not by antagonist of α1-AR or β-AR. Bath application of α2-AR agonist inhibited the MLI-PC synaptic transmission, as well as occluded the effect of NA on the synaptic response. NA-induced depression of the MLI-PC synaptic transmission was completely blocked by a mixture of α2A- and 2B-AR antagonists, as well as abolished by inhibition of protein kinase A (PKA). In addition, electrical stimulation of molecular layer evoked MLI-PC GABAergic synaptic transmission in the presence of AMPA receptor antagonist, which was inhibited by NA through α2-AR. Our results indicate that NA inhibits MLI–PC GABAergic synaptic transmission via an α2-AR/PKA signaling pathway, at least in part through a presynaptic mechanism. These results suggest that the adrenergic neurons in the LC may modulate the output of PCs through regulating MLI–PC synaptic transmission in sensory information transmission of mouse cerebellar cortex.