bluuebunny/crossref_metadata_2025_split huggingface dataset:
Identification of collaborative cross mouse strains permissive to Salmonella enterica serovar Typhi infection
Authors: Alugupalli Kishore R. , Kothari Sudeep , Cravens Matthew P. , Walker Justin A. , Dougharty Darren T. , Dickinson Gregory S. , Gatto Louis A. , Bäumler Andreas J. , Wangdi Tamding , Miller Darla R. , Pardo-Manuel de Villena Fernando , Siracusa Linda D. 
Published: January 2023

AbstractSalmonella enterica serovar Typhi is the causative agent of typhoid fever restricted to humans and does not replicate in commonly used inbred mice. Genetic variation in humans is far greater and more complex than that in a single inbred strain of mice. The Collaborative Cross (CC) is a large panel of recombinant inbred strains which has a wider range of genetic diversity than laboratory inbred mouse strains. We found that the CC003/Unc and CC053/Unc strains are permissive to intraperitoneal but not oral route of S. Typhi infection and show histopathological changes characteristic of human typhoid. These CC strains are immunocompetent, and immunization induces antigen-specific responses that can kill S. Typhi in vitro and control S. Typhi in vivo. Our results indicate that CC003/Unc and CC053/Unc strains can help identify the genetic basis for typhoid susceptibility, S. Typhi virulence mechanism(s) in vivo, and serve as a preclinical mammalian model system to identify effective vaccines and therapeutics strategies.
Biomechanical evaluation of position and bicortical fixation of anterior lateral vertebral screws in a porcine model
Authors: Hsieh Ming-Kai , Lee De-Mei , Li Yun-Da , Peng Chun-Chin , Tsai Tsung-Ting , Lai Po-Liang , Chen Weng-Pin , Tai Ching-Lung 
Published: January 2023

AbstractAlthough an anterior approach with anterior lateral screw fixation has been developed for stabilizing the thoracolumbar spine clinically, screw loosening still occurs. In this novel in vitro study, we attempted to elucidate the optimal screw position in the lateral lumbar vertebra and the effect of bicortical fixation. A total of 72 fresh-frozen lumbar vertebrae from L1–6 were harvested from 12 mature pigs and randomly assigned to two modalities: bicortical fixation (n = 36) and unicortical fixation (n = 36). Six groups of screw positions in the lateral vertebral body in each modality were designated as central-anterior, central-middle, central-posterior, lower-anterior, lower-middle, and lower- posterior; 6 specimens were used in each group. The correlations between screw fixation modalities, screw positions and axial pullout strength were analyzed. An appropriate screw trajectory and insertional depth were confirmed using axial and sagittal X-ray imaging prior to pullout testing. In both bicortical and unicortical fixation modalities, the screw pullout force was significantly higher in the posterior or middle position than in the anterior position (p < 0.05), and there was no significant differences between the central and lower positions. The maximal pullout forces from the same screw positions in unicortical fixation modalities were all significantly lower, decreases that ranged from 32.7 to 74%, than those in bicortical fixation modalities. Our study using porcine vertebrae showed that screws in the middle or posterior position of the lateral vertebral body had a higher pullout performance than those in the anterior position. Posteriorly positioned lateral vertebral screws with unicortical fixation provided better stability than anteriorly positioned screws with bicortical fixation.
Primary isolated right ventricular failure after heart transplantation: prevalence, right ventricular characteristics, and outcomes
Authors: Kaveevorayan Peerapat , Tokavanich Nithi , Kittipibul Veraprapas , Lertsuttimetta Thana , Singhatanadgige Seri , Ongcharit Pat , Sinphurmsukskul Supanee , Ariyachaipanich Aekarach , Siwamogsatham Sarawut , Thammanatsakul Kanokwan , Sritangsirikul Supaporn , Puwanant Sarinya 
Published: January 2023

AbstractTo determine the prevalence, right ventricular (RV) characteristics, and outcomes of primary isolated RV failure (PI-RVF) after heart transplant (HTX). PI-RVF was defined as (1) the need for mechanical circulatory support post-transplant, or (2) evidence of RVF post-transplant as measured by right atrial pressure (RAP) > 15 mmHg, cardiac index of < 2.0 L/min/m2 or inotrope support for < 72 h, pulmonary capillary wedge pressure < 18 mmHg, and transpulmonary gradient < 15 mmHg with pulmonary systolic pressure < 50 mmHg. PI-RVF can be diagnosed from the first 24–72 h after completion of heart transplantation. A total of 122 consecutive patients who underwent HTX were reviewed. Of these, 11 were excluded because of secondary causes of graft dysfunction (GD). PI-RVF was present in 65 of 111 patients (59%) and 31 (48%) met the criteria for PGD-RV. Severity of patients with PI-RVF included 41(37%) mild, 14 (13%) moderate, and 10 (9%) severe. The median onset of PI-RVF was 14 (0–49) h and RV recovery occurred 5 (3–14) days after HTX. Severe RV failure was a predictor of 30-day mortality (HR 13.2, 95% CI 1.6–124.5%, p < 0.001) and post-transplant dialysis (HR 6.9, 95% CI 2.0–257.4%, p = 0.001). Patients with moderate PI-RVF had a higher rate of 30-day mortality (14% vs. 0%, p = 0.014) and post-operative dialysis (21% vs. 2%, p = 0.016) than those with mild PI-RVF. Among patients with mild and moderate PI-RVF, patients who did not meet the criteria of PGD-RV had worsening BUN/creatinine than those who met the PGD-RV criteria (p < 0.05 for all). PI-RVF was common and can occur after 24 h post-HTX. The median RV recovery time was 5 (2–14) days after HTX. Severe PI-RVF was associated with increased rates of 30-day mortality and post-operative dialysis. Moderate PI-RVF was also associated with post-operative dialysis. A revised definition of PGD-RV may be needed since patients who had adverse outcomes did not meet the criteria of PGD-RV.
Measurement of aerodynamic force and moment acting on a javelin using a magnetic suspension and balance system
Authors: Seo Kazuya , Okuizumi Hiroyuki , Konishi Yasufumi , Kobayashi Takuto , Hasegawa Hiroaki , Obayashi Shigeru 
Published: January 2023

AbstractThe rules governing the dimensions of the Javelin were substantially changed in 1986. It was considered that this new design guaranteed there was zero pitching moment at 0° angle of attack and that the pitching moment decreased (became negative) with increasing angle of attack. The objective of this study is to investigate if the pitching moment remains always negative (nose-down rotation). To measure accurate aerodynamic forces acting on a Javelin, the world’s largest 1 m magnetic suspension and balance system was used. The magnetic suspension and balance system was able to measure aerodynamic forces without support interference in the wind tunnel. In addition, computational fluid dynamics were carried out to estimate the pitching moment coefficients. It was found that the pitching moment coefficient of a commercially available Javelin becomes positive (nose-up rotation) at lower angles of attack, less than 12°. The pitching moment becomes positive if the upstream side of the center of gravity receives more inflow than the downstream side. This situation can be attained by, for example, increasing the thickness of the upstream side when compared with that of the downstream side.
Neutrophil-to-lymphocyte ratio predicts nodal involvement in unfavourable, clinically nonmetastatic prostate cancer patients and overall survival in pN1 patients
Authors: Zapała Piotr , Garbas Karolina , Lewandowski Zbigniew , Ślusarczyk Aleksander , Ślusarczyk Cezary , Mielczarek Łukasz , Marra Giancarlo , Pradere Benjamin , Rajwa Pawel , Zapała Łukasz , Radziszewski Piotr 
Published: January 2023

AbstractThe aim of our study was to determine the clinical utility of neutrophil-to-lymphocyte ratio (NLR) in predicting presence and prognosis of nodal involvement in patients treated with radical prostatectomy (RP) due to prostate cancer. This single-centre retrospective study included 205 patients treated with RP and lymphadenectomy between 2012 and 2018. Logistic regression and Kaplan–Meier analyses were performed to evaluate the prognostic value of preoperative NLR in terms of nodal spread and survival. Patients staged pN1 presented lower mean NLR (2.53 vs 3.86; p = 0.0025) compared to pN0 patients. On multivariable analysis of different haematological markers, only NLR exceeding the median (≥ 2.7) predicted pN1 (OR = 0.38; p = 0.0367) independently of biopsy grading and PSA. In internal validation (n = 31 pN1, n = 174 pN0) on the bootstrapped dataset using a spare cutoff of NLR ≥ 4.1 would allow sparing lymphadenectomy in 22.09% pN0 patients, missing 6.45% pN1 (NPV 92.66%; 95% CI 84.91–100%). Noticeably, in pN1 patients NLR ≥ 2.7 correlated with shorter overall survival (p = 0.0196), despite its association with reduced risk of pN1. High pre-prostatectomy NLR was negatively associated with pN1, yielding high NPV in internal validation. Simultaneously, high NLR in pN1 patients was associated with shorter survival.
Clinico-epidemiological profile and risk factors of hypertensive crisis among patients attended at a tertiary care hospital in Somalia
Authors: Mohamud Mohamed Farah Yusuf 
Published: January 2023

AbstractHypertensive crisis (HC) is a life-threatening clinical condition in which an abrupt rise in arterial blood pressure can lead to acute damage to vital organs. The main objective of our study is to determine the epidemiological profile, clinical characteristics, and risk factors of hypertensive crisis patients in Somalia. This study was a prospective cross-sectional study conducted on HC patients attended at Mogadishu Somali Turkish Training and Research Hospital in Mogadishu, Somalia, from November 2020 to April 2021. A total of 6239 patients were screened during the study period. The prevalence of HC was 2.1% (128/6239). Of them, 76 (59.4%) were males. The mean (SD) age of the participants was 56.5 (± 16.9) years (range: 24–98 years). 54.7% (70/128) met the criteria for a hypertensive emergency, while 45.3% (58/128) met the criteria of hypertensive urgency. Most patients (55.5%) took a single antihypertensive medicine, with calcium channel blockers being the most frequently used (57.8%). Headache and palpitation were the most often reported symptoms upon admission (39.1% and 25%). The most often prescribed antihypertensive drugs for the initial therapy included Intravenous furosemide (35.2%), Sublingual captopril (25.8%), intravenous nitroglycerin (23.4%), and intravenous labetalol (20%). Among the forms or patterns of end-organ damage of HE, we most frequently observed acute heart failure (45.7%), acute pulmonary edema (29.9%), and acute renal injury (25.7%). Infrequent medical checkups, poor compliance with medications, poor compliance with exercise, positive family history of hypertension, and being male gender were significant predictors of HC, AOR = 20.312; p < 0.000, AOR = 7.021; p < 0.008, AOR = 6.158; p < 0.017, AOR = 3.545; p < 0.032, and AOR = 2.144; p = 0.001, respectively. In Somalia, the hypertensive crisis is common in this clinic population. Infrequent medical checkups, poor compliance with medications and exercise, positive family history of hypertension, and being male gender were significant predictors of HC.
High-fat diet and estrogen modulate the gut microbiota in a sex-dependent manner in mice
Authors: Hases Linnea , Stepanauskaite Lina , Birgersson Madeleine , Brusselaers Nele , Schuppe-Koistinen Ina , Archer Amena , Engstrand Lars , Williams Cecilia 
Published: January 2023

AbstractA high-fat diet can lead to gut microbiota dysbiosis, chronic intestinal inflammation, and metabolic syndrome. Notably, resulting phenotypes, such as glucose and insulin levels, colonic crypt cell proliferation, and macrophage infiltration, exhibit sex differences, and females are less affected. This is, in part, attributed to sex hormones. To investigate if there are sex differences in the microbiota and if estrogenic ligands can attenuate high-fat diet-induced dysbiosis, we used whole-genome shotgun sequencing to characterize the impact of diet, sex, and estrogenic ligands on the microbial composition of the cecal content of mice. We here report clear host sex differences along with remarkably sex-dependent responses to high-fat diet. Females, specifically, exhibited increased abundance of Blautia hansenii, and its levels correlated negatively with insulin levels in both sexes. Estrogen treatment had a modest impact on the microbiota diversity but altered a few important species in males. This included Collinsella aerofaciens F, which we show correlated with colonic macrophage infiltration. In conclusion, male and female mice exhibit clear differences in their cecal microbial composition and in how diet and estrogens impact the composition. Further, specific microbial strains are significantly correlated with metabolic parameters.
Shift in groundwater recharge of the Bengal Basin from rainfall to surface water
Authors: Jameel Yusuf , Stahl Mason , Michael Holly , Bostick Benjamin C , Steckler Michael S. , Schlosser Peter , van Geen Alexander , Harvey Charles 
Published: January 2023

AbstractGroundwater supports agriculture and provides domestic water for over 250 million people in the Bengal Basin. Here we investigate the source of groundwater recharge using over 2500 stable water isotope measurements from the region. We employ a Monte Carlo statistical analysis to find distributions of possible components of recharge by accounting for the variability of isotope ratios in each of the possible recharge sources. We find that groundwater recharge sources have shifted in the last decades with a ~50% increase in recharge from stagnant surface water bodies (mostly during the latter part of the dry season) and a relative decrease in contribution from direct infiltration of precipitation (which occurs mostly in the early monsoon). We attribute this shift to an increase in standing water in irrigated rice fields and ponds, and an increase in the downward hydraulic gradient during the dry season driven by pumping.
piRNAs regulate a Hedgehog germline-to-soma pro-aging signal
Authors: Shi Cheng , Murphy Coleen T. 
Published: January 2023

AbstractThe reproductive system regulates somatic aging through competing anti- and pro-aging signals. Germline removal extends somatic lifespan through conserved pathways including insulin and mammalian target-of-rapamycin signaling, while germline hyperactivity shortens lifespan through unknown mechanisms. Here we show that mating-induced germline hyperactivity downregulates piRNAs, in turn desilencing their targets, including the Hedgehog-like ligand-encoding genes wrt-1 and wrt-10, ultimately causing somatic collapse and death. Germline-produced Hedgehog signals require PTR-6 and PTR-16 receptors for mating-induced shrinking and death. Our results reveal an unconventional role of the piRNA pathway in transcriptional regulation of Hedgehog signaling and a new role of Hedgehog signaling in the regulation of longevity and somatic maintenance: Hedgehog signaling is controlled by the tunable piRNA pathway to encode the previously unknown germline-to-soma pro-aging signal. Mating-induced piRNA downregulation in the germline and subsequent Hedgehog signaling to the soma enable the animal to tune somatic resource allocation to germline needs, optimizing reproductive timing and survival.
Challenging Cases of Neurocognitive Disorders
Authors: Mastrianni James A. , Seibert Kaitlin 
Published: January 2023

AbstractDementia is broadly defined by DSM-V as cognitive decline from a previous level that impacts the patient's functioning at work or play. This broad definition does not provide information about the underlying disease process, an aspect of clinical care that is of increasing importance, as therapeutic development inches closer to effective disease-modifying treatments. The most common neurodegenerative dementias include Alzheimer's disease, dementia with Lewy bodies, frontotemporal dementia, and Parkinson's disease dementia. Although rare, the prion diseases constitute an important group of dementias that should be routinely considered in the evaluation. Over the last two decades, advances in neuroimaging, biomarker development, and neurogenetics have not only led to a better understanding of the biology of these diseases, but they have improved our awareness of less common clinical subtypes of dementia. As such, to best define the disease process, the evaluation of a patient with cognitive decline requires attention to a myriad of disease aspects, such as the primary symptom at onset (memory, language, visual perception, praxis, etc.), the age at onset (younger or older than 65 years), the rate of disease progression (weeks to months or years), the cognitive and behavioral profile (neuropsychological assessment), and involvement of physical findings. We present here three cases that highlight the decision-making process in the evaluation of patients with atypical presentations of dementia.
Polariton lasing in AlGaN microring with GaN/AlGaN quantum wells
Authors: Delphan Anthonin , Makhonin Maxim N. , Isoniemi Tommi , Walker Paul M. , Skolnick Maurice S. , Krizhanovskii Dmitry N. , Skryabin Dmitry V. , Carlin Jean-François , Grandjean Nicolas , Butté Raphaël 
Published: January 2023

Microcavity polaritons are strongly interacting hybrid light–matter quasiparticles, which are promising for the development of novel light sources and active photonic devices. Here, we report polariton lasing in the UV spectral range in microring resonators based on GaN/AlGaN slab waveguides, with experiments carried out from 4 K up to room temperature. Stimulated polariton relaxation into multiple ring resonator modes is observed, which exhibit threshold-like dependence of the emission intensity with pulse energy. The strong exciton-photon coupling regime is confirmed by the significant reduction of the free spectral range with energy and the blueshift of the exciton–like modes with increasing pulse energy. Importantly, the exciton emission shows no broadening with power, further confirming that lasing is observed at electron–hole densities well below the Mott transition. Overall, our work paves the way toward the development of novel UV devices based on the high-speed slab waveguide polariton geometry operating up to room temperature with the potential to be integrated into complex photonic circuits.
Aging temperature effects on microstructure and mechanical properties for additively manufactured AlSi10Mg
Authors: Yaru Liang , Tiejun Ma , Tounan Jin , Bo Zhang , Le Yang , Wenhang Yin , Hanguang Fu 
Published: January 2023

AlSi10Mg alloy was prepared by laser powder bed fusion (LPBF). The evolution of microstructure and mechanical properties after diverse aging temperatures were systematically studied. The metastable ultra-fine cellular structure was obtained for as-built specimens. Aging at lower temperatures (100–225°C) promotes the formation of a large number of Si-rich precipitates, and the original Si networks can be retained. With the increase in aging temperature, Si network gradually broke apart, and Si-rich particles grew and spheroidised. The hardness increases with temperatures up to 175°C, and decreases thereafter, while the opposite trend was observed for elongation. Excellent comprehensive mechanical properties with a tensile strength of 381 MPa and elongation of 5.2% can be obtained at 225°C for 2 h. Highlights The effect of aging temperatures on microstructure stability and mechanical properties of additive manufacturing AlSi10Mg alloy has been revealed systematically. The Si-rich precipitates and eutectic Si network were responsible for the observed mechanical properties changes. A good combination of strength and ductility is obtained at 225°C for 2 h aging heat treatment.
Effect of lanthanum on thermal and mechanical properties of modified polyvinyl chloride film
Authors: Chen Yan , Lu Yi-Heng , Lu Yu-Wei 
Published: January 2023

Abstract
Polyvinyl chloride (PVC) hybrid film containing cerium, tin and folic acid modified nano titanium dioxide is a new functional material with good compatibility, light resistance and heat resistance. In this work, the PVC hybrid film was prepared by replacing cerium with lanthanum. The hybrid film was characterized by thermogravimetric infrared spectroscopy, dynamic mechanical analysis, atomic force microscopy, x-ray photoelectron spectroscopy, scanning electron microscopy, tensile strength and thermal aging. The results show that the initial thermal stability of the hybrid film containing lanthanum is slightly higher than that of the control sample (conversion α < 1.9%). For the same gaseous component plasticizer, the peak intensity of the ester based infrared characteristic group is higher than that of the hybrid film containing lanthanum, indicating that the intermolecular force between lanthanum and plasticizer is greater than that of cerium. In the range of 350 °C–575 °C and the wave number range of 2850–3100 cm−1, the peak height of the lanthanum containing film is significantly higher than that of the control sample, which may be due to the difference of the outer layer electrons. The peak intensity of O and C atoms on the surface of the hybrid film containing lanthanum is significantly lower than that of the control sample, indicating that the surface relative concentration of lanthanum atoms is lower than that of cerium atoms. The surface roughness RMS of the hybrid film containing lanthanum is significantly lower than that of the organic tin film, which is the result of the attraction between folic acid modified TiO2, lanthanum and tin and the carbon chlorine bond in PVC. It has the function of a potential ultrafiltration membrane. In addition, the hybrid film containing 1phr lanthanum shows good energy storage modulus and low temperature resistance. The prepared hybrid film shows the characteristics of a potential multi-functional material.
Machine-learning assisted compositional optimization of 2xxx series aluminum alloys towards tensile strength
Authors: Zhang Yingbo , Zhang Pu , Li Jiaheng , Zeng Qi , Li Mojia , Hu Yunfeng , Peng Yuanhui 
Published: January 2023

Abstract
High-strength 2xxx series aluminum alloys (Al-Cu system) have been favored by the aerospace and railway transportation industries. Traditionally, developing new materials with targeted properties is guided by extensive experiments and expert experience, causing the development process to be dismayingly slow and expensive. Here, a Kriging model-based efficient global optimization(EGO) lgorithm is applied to search for new 2xxx series aluminum alloys with high tensile strength in a huge search space. After four iterations, the alloy’s ultimate tensile strength increased by 60 MPa, which is higher than that of the best alloy in the initial data set. This study demonstrates the feasibility of using machine-learning to search for 2xxx alloys with good mechanical performance.