bluuebunny/crossref_metadata_2025_split huggingface dataset:
Early adversity and inflammation at midlife: the moderating role of internalizing psychopathology
Authors: Michal Zachary S. , Marquardt Craig A. , Krueger Robert F. , Arbisi Paul A. , Venables Noah C. 
Published: January 2025

AbstractBackgroundChildhood adversity has been associated with increased peripheral inflammation in adulthood. However, not all individuals who experience early adversity develop these inflammatory outcomes. Separately, there is also a link between various internalizing emotional distress conditions (e.g. depression, anxiety, and fear) and inflammation in adulthood. It is possible the combination of adult emotional distress and past childhood adversity may be uniquely important for explaining psychopathology-related immune dysfunction at midlife.MethodsUsing data from the Midlife in the United States (MIDUS) study (n = 1255), we examined whether internalizing, defined as past 12-month emotional distress symptomatology and trait neuroticism, moderated associations between childhood adversity and heightened inflammation in adulthood. Using latent variable modeling, we examined whether transdiagnostic or disorder-specific features of emotional distress better predicted inflammation.ResultsWe observed that childhood adversity only predicted adult inflammation when participants also reported adult internalizing emotional distress. Furthermore, this moderation effect was specific to the transdiagnostic factor of emotional distress rather than the disorder-specific features.ConclusionsWe discuss the possibility that adult internalizing symptoms and trait neuroticism together may signal the presence of temporally stable vulnerabilities that amplify the impact of childhood adversity on midlife immune alterations. The study highlights the importance of identifying emotional distress in individuals who have experienced childhood adversity to address long-term immune outcomes and enhance overall health.
From ‘remain’ to ‘become’: the history of bliva in Swedish
Authors: Skrzypek Dominika , Engdahl Elisabet 
Published: January 2025

Abstract
In Old Swedish, the verb varda ‘become’ was used both as a copula and as a passive auxiliary. During the 1300s, a period of close contact with Middle Low German, the verb blîven ‘remain’ was borrowed into Swedish as bliva. Despite the difference in meaning (‘become’ vs. ‘remain’), the use of bliva increased, and by the end of the 1500s it was used in all constructions where varda was originally found. We study this development in a collection of Swedish texts from 1487 to 1585. The first occurrences of the ‘become’ meaning are found in constructions with adjectival complements, in particular in the highly frequent phrase bliva död ‘become dead (die)’. Once bliva had acquired the meaning ‘become’, it could also be used as a passive auxiliary, which led to a rapid increase in occurrences and subsequently to bliva replacing varda in all contexts.
Achieving excellence in paediatric cardiac care in resource limited and resource plentiful settings and building successful care networks across different countries
Authors: McMahon Colin J. , Penny Daniel J. , Kim Michael , Jacobs Jeffrey P. , Casey Frank , Kumar Raman Krishna 
Published: January 2025

AbstractBackground:The delivery of paediatric cardiac care across the world occurs in settings with significant variability in available resources. Irrespective of the resources locally available, we must always strive to improve the quality of care we provide to our patients and simultaneously deliver such care in the most efficient and cost-effective manner. The development of cardiac networks is used widely to achieve these aims.Methods:This paper reports three talks presented during the 56th meeting of the Association for European Paediatric and Congenital Cardiology held in Dublin in April 2023.Results:The three talks describe how centres of congenital cardiac excellence can be developed in low-income countries, middle-income countries, and well-resourced environments, and also reports how centres across different countries can come together to collaborate and deliver high-quality care. It is a fact that barriers to creating effective networks may arise from competition that may exist among programmes in unregulated and especially privatised health care environments. Nevertheless, reflecting on the creation of networks has important implications because collaboration between different centres can facilitate the maintenance of sustainable programmes of paediatric and congenital cardiac care.Conclusion:This article examines the delivery of paediatric and congenital cardiac care in resource limited environments, well-resourced environments, and within collaborative networks, with the hope that the lessons learned from these examples can be helpful to other institutions across the world. It is important to emphasise that irrespective of the differences in resources across different continents, the critical principles underlying provision of excellent care in different environments remain the same.
The added value of metadata on test completion time for the quantification of cognitive functioning in survey research
Authors: Nichols Emma , Markot Michael , Gross Alden L. , Jones Richard N. , Meijer Erik , Schneider Stefan , Lee Jinkook 
Published: January 2025

Abstract

Objective:
Information on the time spent completing cognitive testing is often collected, but such data are not typically considered when quantifying cognition in large-scale community-based surveys. We sought to evaluate the added value of timing data over and above traditional cognitive scores for the measurement of cognition in older adults.

Method:
We used data from the Longitudinal Aging Study in India-Diagnostic Assessment of Dementia (LASI-DAD) study (N = 4,091), to assess the added value of timing data over and above traditional cognitive scores, using item-specific regression models for 36 cognitive test items. Models were adjusted for age, gender, interviewer, and item score.

Results:
Compared to Quintile 3 (median time), taking longer to complete specific items was associated (p < 0.05) with lower cognitive performance for 67% (Quintile 5) and 28% (Quintile 4) of items. Responding quickly (Quintile 1) was associated with higher cognitive performance for 25% of simpler items (e.g., orientation for year), but with lower cognitive functioning for 63% of items requiring higher-order processing (e.g., digit span test). Results were consistent in a range of different analyses adjusting for factors including education, hearing impairment, and language of administration and in models using splines rather than quintiles.

Conclusions:
Response times from cognitive testing may contain important information on cognition not captured in traditional scoring. Incorporation of this information has the potential to improve existing estimates of cognitive functioning.
Double trouble: two retrotransposons triggered a cascade of invasions in Drosophila species within the last 50 years
Authors: Scarpa Almorò , Pianezza Riccardo , Gellert Hannah R. , Haider Anna , Kim Bernard Y. , Lai Eric C. , Kofler Robert , Signor Sarah 
Published: January 2025

AbstractHorizontal transfer of genetic material in eukaryotes has rarely been documented over short evolutionary timescales. Here, we show that two retrotransposons, Shellder and Spoink, invaded the genomes of multiple species of the melanogaster subgroup within the last 50 years. Through horizontal transfer, Spoink spread in D. melanogaster during the 1980s, while both Shellder and Spoink invaded D. simulans in the 1990s. Possibly following hybridization, D. simulans infected the island endemic species D. mauritiana (Mauritius) and D. sechellia (Seychelles) with both TEs after 1995. In the same approximate time-frame, Shellder also invaded D. teissieri, a species confined to sub-Saharan Africa. We find that the donors of Shellder and Spoink are likely American Drosophila species from the willistoni, cardini, and repleta groups. Thus, the described cascade of TE invasions could only become feasible after D. melanogaster and D. simulans extended their distributions into the Americas 200 years ago, likely aided by human activity. Our work reveals that cascades of TE invasions, likely initiated by human-mediated range expansions, could have an impact on the genomic and phenotypic evolution of geographically dispersed species. Within a few decades, TEs could invade many species, including island endemics, with distributions very distant from the donor of the TE.
Water, sanitation, handwashing, and nutritional interventions can reduce child antibiotic use: evidence from Bangladesh and Kenya
Authors: Ercumen Ayse , Mertens Andrew N. , Butzin-Dozier Zachary , Jung Da Kyung , Ali Shahjahan , Achando Beryl S. , Rao Gouthami , Hemlock Caitlin , Pickering Amy J. , Stewart Christine P. , Tan Sophia T. , Grembi Jessica A. , Benjamin-Chung Jade , Wolfe Marlene , Ho Gene G. , Rahman Md. Ziaur , Arnold Charles D. , Dentz Holly N. , Njenga Sammy M. , Meerkerk Theodora , Chen Belinda , Nadimpalli Maya , Islam Mohammad Aminul , Hubbard Alan E. , Null Clair , Unicomb Leanne , Rahman Mahbubur , Colford John M. , Luby Stephen P. , Arnold Benjamin F. , Lin Audrie 
Published: January 2025

AbstractAntibiotics can trigger antimicrobial resistance and microbiome alterations. Reducing pathogen exposure and undernutrition can reduce infections and antibiotic use. We assess effects of water, sanitation, handwashing (WSH) and nutrition interventions on caregiver-reported antibiotic use in Bangladesh and Kenya, longitudinally measured at three timepoints among birth cohorts (ages 3–28 months) in a cluster-randomized trial. Over 50% of children used antibiotics at least once in the 90 days preceding data collection. In Bangladesh, the prevalence of antibiotic use was 10–14% lower in groups receiving WSH (prevalence ratio [PR] = 0.90 (0.82–0.99)), nutrition (PR = 0.86 (0.78–0.94)), and nutrition+WSH (PR = 0.86 (0.79–0.93)) interventions. The prevalence of using antibiotics multiple times was 26–35% lower in intervention arms. Reductions were largest when the birth cohort was younger. In Kenya, interventions did not affect antibiotic use. In this work, we show that improving WSH and nutrition can reduce antibiotic use. Studies should assess whether such reductions translate to reduced antimicrobial resistance.
The blood pressure lowering effect of beetroot juice is impaired in periodontitis and recovered after periodontal treatment
Authors: Sanchez-Orozco Nydia Y. , Rosier Bob T. , Ruiz-Gutierrez Alondra , Marquez-Sandoval Fabiola , Artacho Alejandro , Carrera-Quintanar Lucrecia , Mira Alex 
Published: January 2025

AbstractWe have previously demonstrated that subgingival levels of nitrate-reducing bacteria, as well as the in vitro salivary nitrate reduction capacity (NRC), were diminished in periodontitis patients, increasing after periodontal treatment. However, it remains unclear if an impaired NRC in periodontitis can affect systemic health. To determine this, the effect of nitrate-rich beetroot juice (BRJ) on blood pressure was determined in 15 periodontitis patients before and 70 days after periodontal treatment (i.e., professional mechanical plaque removal, oral hygiene instruction, and subgingival instrumentation), as well as in a healthy control group of 15 individuals. Additionally, subgingival and tongue samples were taken to analyse the bacterial composition with Illumina sequencing of the 16S rRNA gene. In healthy individuals, the systolic and diastolic blood pressure (SBP and DPB) decreased significantly (both P < 0.01) 90 min after BRJ intake, but not in periodontitis patients. However, after periodontal treatment, this blood pressure-lowering effect was recovered (P < 0.05 for SBP; P < 0.01 for DBP). Lower levels of salivary nitrate after identical doses of BRJ intake indicated a potentially higher NRC in healthy individuals (P < 0.05). Periodontitis-associated bacteria decreased in tongue and subgingival samples after periodontal treatment (P < 0.01). In contrast, nitrate-reducing bacteria were associated with health in both habitats, but increased only in subgingival plaque after periodontal treatment (P < 0.001). This is the first study showing that periodontitis could limit the blood-pressure lowering effects of nitrate reduction by the oral microbiota. We propose that an impaired NRC represents a potential link between periodontitis and systemic conditions, which should be confirmed in future randomized controlled trials. Future work should also aim to determine if nitrate prebiotic supplementation and/or tongue cleaning could improve the treatment of periodontitis and its associated comorbidities.
Synaptic vesicle characterization of iPSC-derived dopaminergic neurons provides insight into distinct secretory vesicle pools
Authors: Fujise Kenshiro , Mishra Jaya , Rosenfeld Martin Shaun , Rafiq Nisha Mohd 
Published: January 2025

AbstractThe dysfunction of dopaminergic (DA) neurons is central to Parkinson’s disease. Distinct synaptic vesicle (SV) populations, differing in neurotransmitter content (dopamine vs. glutamate), may vary due to differences in trafficking and exocytosis. However, the structural organization of these vesicles remains unclear. In this study, we examined axonal varicosities in human iPSC-derived DA and glutamatergic neurons (i3Neurons). i3Neurons primarily contained small, clear SVs (40–50 nm), whereas DA neurons contained larger, pleiomorphic vesicles including dense core and empty vesicles, in addition to the classical SVs. VMAT2-positive vesicles in DA neurons, which load dopamine, were spatially segregated from VGLUT1/2-positive vesicles in an SV-like reconstitution system. These vesicles also colocalized with SV markers (e.g., VAMP2, SV2C), and can be clustered by synapsin. Moreover, DA axonal terminals in mouse striata showed similar vesicle pool diversity. These findings reveal structural differences in DA neurons’ vesicles, highlighting iPSC-derived neurons as effective models for studying presynaptic structures.
Assessment of the predictive power the radiation-induced lymphocyte apoptosis method in prostate cancer patients
Authors: Sándor Gyöngyvér Orsolya , Farkas Gyöngyi , Székely Gábor , Ágoston Péter , Jorgo Kliton , Gesztesi László , Major Tibor , Pesznyák Csilla , Herein András , Stelczer Gábor , Mihály Dalma , Fröhlich Georgina , Jurányi Zsolt , Takácsi-Nagy Zoltán , Polgár Csaba , Kocsis Zsuzsa S. 
Published: January 2025

AbstractDue to the better survival of patients with tumorous diseases, it is increasingly important to predict the side effects of radiotherapy, for which the Radiation-Induced Lymphocyte Apoptosis (RILA) method is proving to be effective in multicentric studies. Prostate cancer is the leading cause of cancer-related deaths among men worldwide, which is usually treated with radiotherapy. We recruited 49 patients with localized prostate cancer and performed RILA measurements before radiotherapy. Patients were treated with CyberKnife (35–40 Gy) or high-dose-rate brachytherapy (1 × 21 Gy). We performed chromosome aberration test for five years and graded side effects, IPSS and QoL questionnaire scores were recorded. We found that RILA% correlated with the IPSS increase (p = 0.0016, r=-0.44), which was confirmed with negative binomial regression (p = 0.0013). The negative predictive value for severe urinary side effects according to the IPSS questionnaire was 87.9% at the lower tertile of RILA values. Chromosome aberrations also correlated with side effects, but when we built models including variables of baseline and treatment characteristics of the patients, RILA, and chromosome aberrations, only RILA predicted a late increase in IPSS score (p < 0.0001, adjusted R2 = 68%). The RILA method was shown to be predictive of urinary side effects especially of patient-reported outcomes.
Modelling mixed crop-livestock systems and climate impact assessment in sub-Saharan Africa
Authors: Srivastava Amit Kumar , Rahimi Jaber , Alsafadi Karam , Vianna Murilo , Enders Andreas , Zheng Wenzhi , Demircan Alparslan , Dieng Mame Diarra Bousso , Salack Seyni , Faye Babacar , Singh Manmeet , Halder Krishnagopal , Ewert Frank , Gaiser Thomas 
Published: January 2025

AbstractClimate change significantly challenges smallholder mixed crop-livestock (MCL) systems in sub-Saharan Africa (SSA), affecting food and feed production. This study enhances the SIMPLACE modeling framework by incorporating crop-vegetation-livestock models, which contribute to the development of sustainable agricultural practices in response to climate change. Applying such a framework in a domain in West Africa (786,500 km2) allowed us to estimate the changes in crop (Maize, Millet, and Sorghum) yield, grass biomass, livestock numbers, and greenhouse gas emission in response to future climate scenarios. We demonstrate that this framework accurately estimated the key components of the domain for the past (1981–2005) and enables us to project their future changes using dynamically downscaled Global Circulation Model (GCM) projections (2020–2050). The results demonstrate that in the future, the northern part of the study area will likely experience a significant decline in crop biomass (up to -56%) and grass biomass (up to -57%) production leading to a decrease in livestock numbers (up to -43%). Consequently, this will impact total emissions (up to -47% CH4) and decrease of -41% in milk production, and − 47% in meat production concentrated in the Sahelian zone. Whereas, in pockets of the Sudanian zone, an increase in livestock population and CH4 emission of about + 24% has been estimated, indicating that variability in climate change impact is amplifying with no consistent pattern evident across the study domain.
Serological insights from SARS-CoV-2 heterologous prime and boost responses in Thailand
Authors: Ward Daniel , Pattarapreeyakul Lapasrada , Pitaksalee Rujiraporn , Thawong Naphatcha , Sawaengdee Waritta , Tuntigumthon Suthida , Patterson Catriona , Tetteh Kevin , Campino Susana , Dhepakson Panadda , Mahasirimongkol Surakameth , Clark Taane G. 
Published: January 2025

AbstractDuring the COVID-19 pandemic, heterologous vaccination strategies were employed to alleviate the strain on vaccine supplies. The Thailand Ministry of Health adopted these strategies using vector, inactivated, and mRNA vaccines. However, this approach has introduced challenges for SARS-CoV-2 sero-epidemiology studies. Our study analysed 647 samples from healthcare workers who received CoronaVac, ChAdOx1 nCoV-19, and BNT162b2 vaccines. The serological profile encompassed responses to various SARS-CoV-2 variants and vectors, measuring IgG, IgM, and IgA isotypes, alongside IgG avidity assays. The results demonstrated that heterologous CoronaVac/ChAdOx1 nCoV-19 schedules elicited significantly stronger antibody responses compared to homologous schedules (IgG: 1.2-fold, IgM: 10.9-fold, IgA: 3.1-fold increase). Additionally, a heterologous BNT162b2 boost at 4-weeks post-initial vaccination showed greater antibody levels than a ChAdOx1 nCoV-19 boost (IgG: 1.1-fold, IgM: slight decrease, IgA: 1.5-fold increase). Using a combination of three analytes, IgG against wild-type Spike trimer, nucleoprotein and Omicron receptor binding domains, enabled the clustering of responses within a statistical Gaussian mixture model that successfully discriminates between breakthrough infections and vaccination types (F-score = 0.82). The development of statistical models to predict breakthrough infections can improve serological surveillance. Overall, our study underscores the necessity for vaccine (re-)development and the creation of serological tools to monitor vaccine performance.
The level of cognitive functioning in school-aged children is predicted by resting EEG Directed Phase Lag Index
Authors: Charlebois-Poirier Audrey-Rose , Davoudi Saeideh , Lalancette Ève , Knoth Inga Sophia , Lippé Sarah 
Published: January 2025

AbstractQuantifying cognitive potential relies on psychometric measures that do not directly reflect cortical activity. While the relationship between cognitive ability and resting state EEG signal dynamics has been extensively studied in children with below-average cognitive performances, there remains a paucity of research focusing on individuals with normal to above-average cognitive functioning. This study aimed to elucidate the resting EEG dynamics in children aged four to 12 years across normal to above-average cognitive potential. Our findings indicate that signal complexity, as measured by Multiscale Entropy (MSE), was not significantly predictive of the level of cognitive functioning. However, utilizing Directed Phase Lag Index (DPLI) as an effective connectivity measure, we observed consistent patterns of information flow between anterior and posterior regions. Fronto-parietal as well as local connectivity patterns were seen across most of the cognitive functions. Moreover, specific connectivity patterns were obtained for each intellectual quotient index (namely verbal comprehension, visuospatial, fluid reasoning, and processing speed indexes as well as full-scale intellectual quotient). These results underscore the presence of long-range connections and support fronto-parietal theories of cognitive abilities within the resting state brain dynamics of children.
CRISPR-Enabled Autonomous Transposable Element (CREATE) for RNA-based gene editing and delivery
Authors: Wang Yuxiao , Lin Ruei-Zeng , Harris Meghan , Lavayen Bianca , Diwanji Neha , McCreedy Bruce , Hofmeister Robert , Getts Daniel 
Published: January 2025

Abstract
To address a wide range of genetic diseases, genome editing tools that can achieve targeted delivery of large genes without causing double-strand breaks (DSBs) or requiring DNA templates are necessary. Here, we introduce CRISPR-Enabled Autonomous Transposable Element (CREATE), a genome editing system that combines the programmability and precision of CRISPR/Cas9 with the RNA-mediated gene insertion capabilities of the human LINE-1 (L1) element. CREATE employs a modified L1 mRNA to carry a payload gene, and a Cas9 nickase to facilitate targeted editing by L1-mediated reverse transcription and integration without relying on DSBs or DNA templates. Using this system, we demonstrate programmable insertion of a 1.1 kb gene expression cassette into specific genomic loci of human cell lines and primary T cells. Mechanistic studies reveal that CREATE editing is highly specific with no observed off-target events. Together, these findings establish CREATE as a programmable, RNA-based gene delivery technology with broad therapeutic potential.