bluuebunny/crossref_metadata_2025_split huggingface dataset:
New Constraints on Dark Matter and Cosmic Neutrino Profiles through Gravity
Authors: Tsai Yu-Dai , Eby Joshua , Arakawa Jason , Farnocchia Davide , Safronova Marianna 
Published: January 2023

Abstract
We derive purely gravitational constraints on dark matter and cosmic neutrino profiles in the solar system using asteroid (101955) Bennu. We focus on Bennu because of its extensive tracking data and high-fidelity trajectory modeling resulting from the OSIRIS-REx mission. We find that the local density of dark matter is bound by $\rho_{\rm DM}\lesssim 3.3\times 10^{-15}\;\rm kg/m^3 \simeq 6\times10^6\,\bar{\rho}_{\rm DM}$, in the vicinity of $\sim 1.1$ au (where $\bar{\rho}_{\rm DM}\simeq 0.3\;\rm GeV/cm^3$). We show that high-precision tracking data of solar system objects can constrain cosmic neutrino overdensities relative to the Standard Model prediction $\bar{n}_{\nu}$, at the level of $\eta\equiv n_\nu/\bar{n}_{\nu}\lesssim 1.7 \times 10^{11}(0.1 \;{\rm eV}/m_\nu)$ (Saturn), comparable to the existing bounds from KATRIN and other previous laboratory experiments (with $m_\nu$ the neutrino mass). These local bounds have interesting implications for existing and future direct-detection experiments. Our constraints apply to all dark matter candidates but are particularly meaningful for scenarios including solar halos, stellar basins, and axion miniclusters, which predict overdensities in the solar system. Furthermore, introducing a DM-SM long-range fifth force with a strength $\tilde{\alpha}_D$ times stronger than gravity, Bennu can set a constraint on $\rho_{\rm DM}\lesssim \bar{\rho}_{\rm DM}\left(6 \times 10^6/\tilde{\alpha}_D\right)$. These constraints can be improved in the future as the accuracy of tracking data improves, observational arcs increase, and more missions visit asteroids.
Fate of nitrogen and phosphorus from source-separated human urine in a calcareous soil
Authors: Rumeau Manon , Marsden Claire , Mouheb Nassim Ait , Crevoisier David , Pistocchi Chiara 
Published: January 2023

Abstract
Human urine concentrates 85% of the nitrogen and 65% of the phosphorus excreted by humans, making it a potential alternative crop fertilizer. However, knowledge gaps remain on the fate of nitrogen in situations favouring NH3 volatilisation and on the availability of P from urine. This study aimed at identifying the fate of nitrogen and phosphorus supplied by human urine from source separation toilets in a calcareous soil. To this end, a spinach crop was fertilized with 2 different doses of human urine (170 kgN.ha-1 + 8.5kgP.ha-1 and 510 kgN.ha-1 + 25.5 kgP.ha-1) and compared with a chemical fertiliser treatment (170 kgN.ha-1 + 8.5kgP.ha-1) and a water treatment. The experiment was conducted in 4 soil tanks (50 cm depth) in greenhouse conditions, according to a randomized block scheme. We monitored soil mineral nitrogen over time and simulated nitrogen volatilisation using Hydrus-1D and Visual Minteq softwares. We also monitored soil phosphorus pools, microbial biomass CNP, soil pH and electrical conductivity. Only an excessive input of urine affected soil pH (decreasing it by 0.2 units) and soil conductivity (increasing it by 183%). The phosphorus supplied was either taken up by the crop or remained in the available P pool. Ammonium seemed to be nitrified within about 10 days after application. However, both Visual Minteq and Hydrus models estimated that more than 50% of the nitrogen supplied was lost by ammonia volatilisation. Overall, our results indicate that direct application of urine to a calcareous soil is possible without harming soil properties, but that heavy losses of volatilised nitrogen are to be expected.
Valorization of encapsulated coffee parchment extracts as metabolic control for high fructose diet-induced obesity, using Wistar rat as animal model.
Authors: BENYELLES Meriem , merzouk hafida , IMESSAOUDENE Asmahan , MEDJDOUB Amel , MEBARKI Abdelouaheb 
Published: January 2023

Abstract

Purpose : Parchment is a coffee by-products with high amounts of polyphenols that could be effective in the prevention of obesity-related metabolic disorders. However, their effectiveness depends on their stability and bioavailability which could be overcome by encapsulation. The aim of this study was to investigate anti-obesity effects of aqueous coffee parchment extracts.
Methods : Aqueous coffee parchment extracts were administrated orally (100 mg/kg weight/day) as free or in a microencapsulated form with whey protein/maltodextrin, in fructose-induced obesity rat model (20% fructose in water). Different metabolic parameters were determined.
Results : Parchment extract contained total phenolics at 79.50 mg GAE/ g, with chlorogenic acid and caffeine as main compounds. The microencapsulation efficiency was 89.36% and SEM images revealed spherical microcapsules which exhibited polyphenol release under simulated digestion. Fructose obese rats displayed increased adiposity and metabolic alterations as hyperinsulinemia, hyperglycemia, hyperlipidemia and oxidative stress with high liver and adipose tissue lipids. The treatment with parchment extracts prevented adipose fat accumulation and alleviated the hyperglycemia, hyperinsulinemia, hyperlipidemia, liver steatosis and oxidative stress in obese rats. The effects were more pronounced with encapsulation.
Conclusion : Coffee parchment extracts protected against fructose-induced obesity metabolic alterations ; a better amelioration was observed with microcapsules which will increase the value of this coffee by-product.
Green's function in general relativity
Authors: Kurihara Yoshimasa 
Published: January 2023

Abstract
This report provides Green's functions (classical propagators) of gravitational fields of vierbein and spin-connection fields in general relativity.
The existence of Green's function of the Laplace operator in curved space with an indefinite metric is ensured owing to the Hodge harmonic analysis.
The analyticity of Green's function is naturally determined intrinsically, keeping a causality.
This report proposed a novel definition of the momentum space in curved space-time and the linearisation of the Einstein equation as a free field consistent with that for the Yang-Mills gauge field.
The proposed linearisation does not utilize the weak-field approximation; thus, the method is applicable for highly caved space-time.
We gave two examples of Green's function of gravitational fields, the plane wave solution and the Schwarzschild solution.
Identification of a novel prognostic gene signature in high-grade lung neuroendocrine tumors
Authors: Wang Guige , Guo Chao , Li Shanqing 
Published: January 2023

Abstract
Background
The prognosis of high-grade LNETs patients was poor. Although previous studies have tried to explore molecular markers to stratify LNETs and to seek the therapeutic targets, there was little improvement in the treatment strategy and overall survival of high-grade LNET patients.
Materials and Methods
In our study, gene expression data and clinical features of LNETs patients were extracted from GSE30219 dataset, which was further divided into training cohort and validation cohort. Univariate cox regression analysis and LASSO method were used to construct a prognostic risk model. The predictive value of prognostic gene signature was evaluated by ROC curve analysis.
Results
An eight-gene signature predicting survival was constructed in training cohort and was further validated in validation cohort. This gene signature performed well in both early-stage and advanced-stage high-grade LNET patients. Moreover, multivariate cox regression analysis revealed that this gene signature was the only independent risk factor in high-grade LNET patients, while age, gender, pathological stage, T stage, N stage and M stage were not. GO analysis and KEGG pathway analysis of DEGs and GSEA analysis based on subgroups stratified by gene signature in high-grade LNET patients revealed the high activity of tumor cell division, proliferation and metabolization.
Conclusion
We identified a novel and reliable gene signature risk model for predicting prognosis in high-grade LNET patients, which could stratify high-risk LNETs patients and might provide new targets.
Low dose blood BTEX are associated with pulmonary function through changes in inflammatory markers among US adults: NHANES 2007-2012
Authors: He Yansu , Lin Yong , Qiu Hong , Wu Linying , Ho Kin Fai 
Published: January 2023

Abstract
The effects of blood benzene, toluene, ethylbenzene, and xylenes (BTEX) on lung function among general adults remain unknown. We enrolled 5,519 adults with measured blood BTEX concentrations and lung function from the US National Health and Nutrition Examination Survey 2007–2012. Weighted linear models were fitted to assess the associations of BTEX with lung function and inflammation parameters (white blood cell five-part differential count and C-reactive protein). The mediating effect of inflammation between BTEX and lung function was also examined. Blood BTEX concentrations decreased yearly from 1999 and were extremely low from 2007–2012. Benzene and toluene exerted the greatest influence on lung function in terms of forced vital capacity (FVC), forced expiratory volume in the first second (FEV1), calculated FEV1:FVC ratio, peak expiratory flow rate (PEFR), and forced mid expiratory flow (FEF25%–75%). Both ethylbenzene and all xylene isomers had no effects on FVC but reduced FEV1, FEV1:FVC ratio, PEFR, and FEF25–75%. Weighted quantile analyses demonstrated that BTEX mixture was associated with decreases in FVC, FEV1, FEV1:FVC ratio, PEFR, and FEF25–75%, with benzene weighted most heavily for all lung function parameters. BTEX also increased the levels of inflammation indicated by white blood cell five-part differential count and C-reactive protein, and increased levels of inflammation also reduced lung function. From multiple mediation analysis, inflammation mediated the effects of benzene on FEV1 and PEFR, the effects of toluene on FEV1, and the effects of ethylbenzene on FEV1 and PEFR. Low-dose exposure to BTEX was associated with reduced pulmonary function both in large and small airways. Inflammation could be involved in this pathogenesis.
Extratropical intraseasonal signals along the subtropical westerly jet as a window of opportunity for subseasonal prediction over East Asia in boreal summer
Authors: Yang Jing , Zhu Tao , Vitart Frédéric 
Published: January 2023

Abstract
Previous studies suggest that boreal summer intraseasonal variations along the subtropical westerly jet (SJ), featuring quasi-biweekly periodicity, frequently modulate downstream subseasonal variations over East Asia (EA). Based on subseasonal hindcasts from six dynamical models, this study first discovered that the leading two–three-week prediction skills for surface air temperature (SAT) are improved significantly in summer with the strengthened intraseasonal oscillation along the SJ, which are best demonstrated over the eastern Tibetan Plateau, Southwest Basin, and North China. The reasons are that the enhanced quasi-biweekly wave and its energy dispersion along the SJ cause more regular quasi-biweekly periodic variations of downstream SAT, which potentially increase regional predictability. This study suggests that the strengthened intraseasonal periodic signals along the SJ would enhance the subseasonal predictability in downstream regions, which could provide a window of opportunity for achieving better subseasonal prediction.
Patterns of rockpool molluscs in differing shore exposures
Authors: Gonçalves Inês , Vinagre Catarina , Silva Ana 
Published: January 2023

Abstract
Rockpools are understudied systems of extreme importance due to their proven vulnerability to climate change. Rockpool molluscs play essential ecological roles therein, directly by acting both as prey and predator and, indirectly by influencing the topography via grazing. However, the environmental factors driving their rockpool occupation remain largely unknown. Our study is one of the few to focus on rockpool molluscs community patterns, particularly at a large scale between shores of differing exposure to wave action, being the first focused assessment of rockpool molluscs in Portugal. Overall, 32 intertidal rock pools on four rocky shores were studied during the summer spring tide of 2015. We used multivariate analysis to evaluate the potential impact of sheltered versus exposed wave action, physical pool structure (length, width, and depth), and relationships within the pool community on the abundance and diversity of molluscs. Wave exposure significantly influenced mollusc abundance and diversity. Among the 37 different taxa identified, there was a greater abundance of grazing gastropods (62.2%), followed by bivalves (27.0%) and chitons (10.8%). We suggest that mollusc taxa may have preferences for particular rock pool habitats depending on the type of algae present rather than pool size. We also suggest that species-specific mollusc presence in rockpool can be related to their shell size, aperture, and foot size due to corresponding variation in musculature needed to hold the snail to the substrate in hydrodynamic conditions. Further investigation would benefit from disentangling the potential role of algal pool coverage as a predator refuge for molluscs.
Risk assessment of assisted reproductive technology and parental ages at childbirth for the development of uniparental disomy-mediated imprinting disorders caused by aneuploid gametes
Authors: Hara-Isono Kaori , Matsubara Keiko , Nakamura Akie , Sano Shinichiro , Inoue Takanobu , Kawashima Sayaka , Fuke Tomoko , Yamazawa Kazuki , Fukami Maki , Ogata Tsutomu , Kagami Masayo 
Published: January 2023

Abstract
Background: Our previous study suggested that assisted reproductive technology (ART) may be a possible risk factor for the development of epimutation-mediated imprinting disorders (epi-IDs) for mothers aged ≥ 30 years. However, whether ART or advanced parental age facilitates the development of uniparental disomy-mediated IDs (UPD-IDs) has not yet been investigated.
Results: We enrolled 130 patients with aneuploid UPD-IDs including various IDs confirmed by molecular studies and obtained ART data of the general population and patients with epi-IDs from a robust nationwide database and our previous report, respectively. We compared the proportion of ART-conceived livebirths and maternal childbearing ages between patients with UPD-IDs and the general population or patients with epi-IDs. The proportion of ART-conceived livebirths in patients with aneuploid UPD-IDs was consistent with that in the general population of maternal age ≥ 30 years and was lower than that in the patients with epi-IDs, although there was no significant difference. The maternal childbearing ages of patients with aneuploid UPD-IDs were skewed to the increased ages with several cases exceeding the 97.5th percentile of maternal childbearing ages of the general population, and significantly higher than that of patients with epi-IDs (P < 0.001). In addition, we compared the proportion of ART-conceived livebirths and parental ages at childbirth between patients with UPD-IDs caused by aneuploid oocytes (oUPD-IDs) and that by aneuploid sperms (sUPD-IDs). Almost all ART-conceived livebirths were identified in patients with oUPD-IDs, and both maternal and paternal ages at childbirth were significantly higher in patients with oUPD-IDs than in patients with sUPD-IDs. Because maternal and paternal ages were strongly correlated (r = 0.637, P < 0.001), higher paternal ages in oUPD-IDs were due to the higher maternal ages in this group.
Conclusions: Different from the case of epi-IDs, ART itself is not likely to facilitate the development of aneuploid UPD-IDs. We demonstrated that advanced maternal age can be a risk factor for the development of aneuploid UPD-IDs, particularly oUPD-IDs.
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Interaction between rhizobacterial community assembly and host responses determines poplar salt tolerance
Authors: Liao Yangwenke , Cao Chaoyang , Zhang Qingyue , Zhang Peiyao , Li Xiaogang 
Published: January 2023

Abstract
Background and aims Rhizosphere microbes play important roles in plant toleranceto abiotic stresses. Plants of different genetic backgrounds acquire stress resistance by assembling specific rhizosphere microbial communitiesor typical beneficial microbiota. However, the molecular mechanism by whichplants recruit microbiota in response to environmental stresses is unclear.
MethodsHere, we investigated transcript regulation in three poplar genotypes, namely, Populus davidiana × P. bolleana Loucne (SXY), P. deltoides × P. euramericana'Nanlin 895' (NL895) and P. alba × P. glandulosa '84K' (84K), and their effect on the rhizosphere microbial community in response to salinization.
ResultsThe results showed that SXY exhibited salt tolerance characterized by the best photosynthesis and antioxidant system performance upon salt stress, while salt stress severely damaged the growth and membrane system of 84K. 16S rRNA sequencing revealed the lowest rhizobacterial community diversity associated with SXY compared to 84K and NL895, implying strong enrichment of certain bacterial taxa by the salt tolerant cultivar. Specifically, SXY recruited higher abundances of Hydrogenophaga and Pseudomonas. Furthermore, RNA-seq analysis of roots combined with weighted gene co-expressionnetwork analysis (WGCNA) identified module eigengene (ME)yellow, a module positively related to the SXY-enriched OTUs. KEGG analysis revealedsignificant enrichment of lipid metabolism and amino acid biosynthesis in MEyellow. Twenty-four genes selected from the pathways enriched in MEyellow showed the highest expression levels in SXY.
ConclusionsOur results suggest a “gene expression-rhizosphere-microbiota-salt tolerance” regulatory process in the poplar response to salinization and provide new insights into the mechanisms by which plants reshape the soil microbiome to improve salt tolerance.
WWC1/2 regulate spinogenesis and cognition in mice by stabilizing AMOT
Authors: Cao Runyi , Zhu Rui , Sha Zhao , Qi Sixian , Lei Yubin , Tan Yanfeng , Zhu Yuwen , Wang Yu , Wang Yi , Yu Faxing 
Published: January 2023

Abstract

WWC1 regulates episodic learning and memory, and genetic nucleotide polymorphism ofWWC1is associated with neurodegenerative diseases such as Alzheimer's disease. However, the molecular mechanism through which WWC1 regulates neuronal function remains unclear. Here, we show that WWC1 and its paralogs (WWC2/3) bind directly to angiomotin (AMOT) family proteins (Motins), and recruit USP9X to deubiquitinate and stabilize Motins. Deletion of WWC in different cell types, including neurons, leads to reduced protein levels of Motins. In mice, neuron-specific deletion ofWwc1andWwc2results in lower density of the dendritic spine and impairment of cognitive functions. Interestingly, ectopic expression of AMOT partially rescues the neuronal phenotypes associated withWwc1/2deletion. Thus, WWC proteins modulate spinogenesis and cognition in part by regulating protein stability of Motins.
Health care use and costs among women experiencing interpersonal violence: A retrospective chart review
Authors: Siltala Heli Pauliina , Kuusinen-Laukkala Anneli , Holma Juha Matti 
Published: January 2023

Abstract
This study explored health care use and costs among 184 Finnish women identified as having experienced family violence, sexual violence, or other interpersonal violence in emergency care. Their medical records were analysed two years before and after their identification. The health care costs generated by the family violence victims were 33% higher before identification and 83% higher thereafter than those among the general population. Health care visits were found to increase towards the identification date with a strong linear correlation among victims of family (r = .68, p < .001), sexual (r = .50, p < .001) and other violence (r = .68, p < .001). The health care costs of the family violence victims surpassed the population mean approximately 20 months before their identification date. The health care use among victims of family (r = − .68, p < .001) and sexual violence (r = − .61, p < .001) decreased significantly for two years after identification. For other victims, the health care use declined for the first year after identification (r = − .63, p < .001) but started to increase again after that (r = .41, p = .003). With the steady decline, the health care costs of the family violence victims reached the mean level of the general population at the very end of the two-year follow-up. The results suggest that earlier identification of victims of family violence could significantly decrease the adverse health effects resulting from violence and create notable savings in the health care sector.
Two-Fingers digital rectal examination for anal diseases: protocol of a prospective, diagnostic study
Authors: Zhi Congcong , Guo Hongxin , Li Xin , Shi Yuying , Cheng Yicheng , Liu Ningyuan , Huang Zichen , Zheng Lihua 
Published: January 2023

Abstract

Purpose Anal fistula is an anorectal infectious disease caused by perianal abscess or perianal disease. Surgery is the main treatment method for anal fistula. Accurate anorectal examination is of great significance to reduce the missed diagnosis rate of anal fistula, especially the misdiagnosis of anal fistula with no clear external opening. Two-Fingers digital rectal examination (TF-DRE) is a method improved by Professor Zheng Lihua on the basis of traditional digital anus examination. It has been used since 2012 and has accumulated a lot of clinical experience. However, there is a lack of comprehensive research on the value of TF-DRE for the diagnosis of anal fistula. The main purpose of this study is to compare the difference in the diagnostic value of TF-DRE, traditional digital rectal examination, and anorectal ultrasonography in the diagnosis of anal fistula. Methods For patients who met the inclusion criteria, TF-DRE was performed to explore the number and location of the external orifice, internal orifice, the number of fistula and the relationship with the perianal sphincter, as well as the data recorded by digital rectal examination and anorectal ultrasonography To make a comparison, the final diagnosis results from clinicians during the operation was taken as the gold standard, the diagnostic accuracy of TF-DRE for anal fistula is calculated, and the significance of TF-DRE in the preoperative diagnosis of anal fistula is studied and analyzed. All statistical results were analyzed using SPSS22.0, and P < 0.05 indicated that the difference was statistically significant. Conclusion The research protocol details the advantages of TF-DRE compared with digital rectal examination and anorectal ultrasonography in the diagnosis of anal fistula, and provides clinical evidence for the diagnostic value of TF-DRE in anal fistula. As an innovative anorectal examination method, there is still a lack of high-quality research using scientific methods in the current literature. This study will provide rigorously designed clinical evidence for TF-DRE.