bluuebunny/crossref_metadata_2025_split huggingface dataset:
SEQUENCE ANALYSIS OF HOT SPOT REGIONS OF SPIKE AND RNA‑DEPENDENT‑RNA POLYMERASE (RDRP) GENES OF SARS-COV-2 IN KERMAN, IRAN
Authors: Nasir  , Ramin  , Amin  , Jamal  
Published: July 2023

Background: Mutations in the SARS-CoV-2 genome might influence pathogenicity, transmission rate, and evasion of the host immune system. Therefore, the purpose of this study was to assess the genetic alteration in the receptor binding domain (RBD) of the spike and putative RNA binding site of the RdRp genes of SARS-CoV-2.
Materials and Method: In this cross-sectional study, 45 confirmed COVID-19 patients using qRT-PCR were included and divided into mild, severe, and critical groups based on the severity of the disease. RNA was extracted from nasopharyngeal swab samples using a commercial kit. RT-PCR was performed to amplify the target sequences of the spike and RdRp genes and sequenced them by the Sanger method. Clustal OMEGA, MEGA 11 software, I-mutant tools, and SWISS-MODEL and HDOCK web servers were used for bioinformatics analyses.
Results: The mean age of the patients was 50.68±2.73. The results showed that four of six mutations (L452R, T478K, N501Y, and D614G) in RBD and three of eight in the putative RNA binding site (P214L, E1084D, V1883T) were missense. In the putative RNA binding site, another deletion was discovered. Among missense mutations, N501Y and V1883T were responsible for increasing structural stability, and the others were responsible for decreasing it. The various homology models designed showed that these homologies were like the Wuhan model. The molecular docking analysis revealed that the T478K mutation in RBD had the highest binding affinity. In addition, 35 RBD samples (89.7%) and 33 putative RNA binding site samples (84.6%) were similar to the Delta variant.
Conclusion: Our results indicated that double mutations (T478K and N501Y) in the S protein might increase the binding affinity of SARS-CoV-2 to human ACE2 compared to the wild type (WT) strain. Moreover, variations in the spike and RdRp genes might influence the stability of encoded proteins.
Pengaruh Pemberian Lidokain Intravena Terhadap Perubahan Hemodinamik dan Kadar Norepinefrin Pada Prosedur Laringoskopi dan Intubasi
Authors: Faqri  , Musba A.M. Takdir , Amin Hisbullah , Arif Syafri Kamsul , Salahuddin Andi , Wirawan Nur Surya 
Published: July 2023

Latar Belakang: Komplikasi utama dari manajemen jalan napas di ruang operasi sangat jarang tetapi dapat mengancam jiwa. Telah dilaporkan bahwa pemberian lidokain intravena dapat secara efektif menekan respons hemodinamik terhadap laringoskopi dan intubasi endotrakeal.
Tujuan: untuk mengetahui efek pemberian lidokain intravena terhadap perubahan respons hemodinamik dan kadar norepinefrin pada prosedur laringoskopi dan intubasi.
Metode dan Subjek: Penelitian ini menggunakan desain penelitian uji acak tersamar ganda (Randomized double blind clinical trial). Secara acak, pasien dibagi menjadi 2 kelompok yaitu kelompok A (lidokain intravena) dan kelompok B (plasebo) dengan jumlah sampel masing-masing 20 orang yang memenuhi kriteria inklusi. Dilakukan pengukuran dan pencatatan hemodinamik (Tekanan darah Sistolik, Diastolik dan laju nadi) dan kadar norepinefrin 5 menit sebelum dilakukan intubasi, menit ke-1, dan ke-5 setelah tindakan intubasi.
Hasil: Terdapat perbedaan tekanan darah sistolik (TDS) dan tekanan darah diastolik (TDD) pada kelompok A dan B antar waktu, di mana terjadi penurunan TDS dan TDD pada saat sebelum dilakukan tindakan dibandingkan setelah tindakan (p<0,05). Ditemukan penurunan laju nadi yang tidak signifikan pada kelompok A, di mana hasil sebaliknya ditemukan pada kelompok B (p<0,05). Perbandingan signifikan ditemukan pada perubahan semua indikator hemodinamik antar kelompok (p<0.05). Untuk kadar norepinefrin, tidak ditemukan perubahan signifikan pada kelompok A, sementara pada kelompok B perubahannya signifikan. Perubahan kadar norepinefrin berbeda secara signifikan jika dibandingkan antar kelompok (p=0,007).
Kesimpulan: Pemberian lidokain intravena sebelum prosedur laringoskopi dan intubasi dapat mencegah peningkatan hemodinamik dan peningkatan kadar norepinefrin.
Perbandingan Efektivitas antara Ivabradin 5 mg dengan Bisoprolol 5 mg dalam Menjaga Kestabilan Hemodinamik pada Tindakan Laringoskopi Intubasi
Authors: Putra Dany Surya , Syafri Kamsul Arif  , Syamsu Hilal Salam  , Ramli Ahmad  , Andi Salahuddin  , Muhammad Rum  
Published: July 2023

Background: Laryngoscopy intubation can cause complications in the form of increased blood pressure and heart rate. This can be detrimental to patients with cardiovascular complications, increased intracranial pressure, and vascular anomalies. Various procedures are performed to stabilize hemodynamic fluctuations during laryngoscopy intubation, one of which is the administration of beta-blocking agents (Bisoprolol). In some studies, the administration of Bisoprolol can cause side effects such as hypotension and bradycardia. One drug considered to maintain hemodynamic stability in laryngoscopy intubation with minimal effects is the administration of Ivabradine, which works by regulating diastolic depolarization.
Objective: To compare the effectiveness of Ivabradine 5 mg and Bisoprolol 5 mg in maintaining hemodynamic stability in laryngoscopy intubation.
Methods: This double-blind randomized clinical trial was conducted at RSUP Dr. Wahidin Sudirohusodo Makassar in July-August 2022 with ethical qualifications from the Ethics Committee for Biomedical Research in Humans from the Faculty of Medicine, Hasanuddin University. The sample was the entire population that met the inclusion and exclusion criteria. The data were processed using SPSS 25. The analysis used paired T-test, Mann-Whitney, and Wilcoxon with a 95% confidence level (p<0.005).
Result: There was a significant decrease in the mean arterial pressure and decrease in heart rate after induction (T2-T0) in the Bisoprolol group (p<0.05). The mean arterial pressure at T1-T0, T3-T0, T3-T2 did not statistically significant.
Conclusion: Ivabradine 5 mg was more effective than Bisoprolol 5 mg in maintaining the stability of mean arterial pressure and heart rate in laryngoscopy intubation.
Mesenchymal Umbilical Cord Stem Cells Treatment for Autism
Authors: Susanti Pada Vinski Deby , Cinta Vinski Natasha 
Published: July 2023

The purpose of this case is to see the effects of mesenchymal umbilical cord treatments when applied to patients with Autism. The observations are of patients attending the main clinic, "Celltech Stem Cell and Banking," in Jakarta, Indonesia. Findings on pre-treatments showed abnormally varying behaviors, physically as well as psychologically. The treatments were conducted at the Celltech Stem Cell Lab clinics in Jakarta, based on good reasons that MCSUC are well-sourced, easy to collect and preserved, besides its traits of multi-directional differentiation. Patients undergoing the treatments showed calm and friendly behaviors and of the five cases presented, all fulfilled the pre-set standard criteria of recovery. This research has implications for paving the way for the development of innovative stem cell therapies for children with autism.
Human disturbances and the daytime activity of sympatric otters along equatorial Amazonian rivers
Authors: Norris Darren , Michalski Fernanda 
Published: July 2023

Background
Previous studies suggest coexistence between sympatric neotropical (Lontra longicaudis) and giant otters (Pteronura brasiliensis) maybe facilitated by temporal and spatial differences in activity. Yet, to date there has been no systematic evaluation of activity of these species in sympatry. Here we use extensive multi-year field data to compare temporal and spatial patterns in the diurnal activity of sympatric giant and neotropical otters to answer three questions: Do temporal patterns in daytime river use change in relation to seasonal river levels (low, rising, high and declining river levels), do they change due to human disturbances (boats and fishing nets) and do patterns in neotropical otter activity change due to the presence of the larger sized giant otter?

Methods
Direct observations of both species were recorded using standardized boat surveys along 218 km of rivers over 53 months during nine years (2011–2013 and 2015–2020). Complementary techniques (Generalized Additive Models, Kernel density estimates and non-parametric tests,) were used to compare diurnal activity patterns along rivers subdivided into 41 river reaches.

Results
The presence of giant otters decreased threefold from 67% of the least disturbed reaches (few boats no fishing nets) to 18% of the most disturbed reaches with many boats and fishing nets. In contrast neotropical otter presence nearly doubled from 44% of the least disturbed to 73% of the most disturbed reaches with fewest giant otter detections. Both species were observed across all daytime hours but were observed rarely on the same day. There was no evidence to suggest simultaneous use of the same reach. When species were detected on the same day, they were separated spatially (median distance between species 12.5 km) and temporally (median time difference 3.0 hours). There was little change in activity of either species among seasons. Giant otters were less active in river reaches with fishing nets and boat use, whereas neotropical otter activity did not appear to be strongly affected by these activities.

Conclusions
Our findings support evidence that diurnal activity in both otter species is flexible, with daytime activity changing due to human disturbances in the case of giant otters.
Engagement with a local African-Caribbean community to explore perspectives and research priorities among Black men for prostate cancer using a co-participatory approach
Authors: Huang Qizhi , Grossett Valerie , Daley Sheila , Fryer Kate , Brown Janet , Mitchell Caroline 
Published: July 2023

BackgroundProstate cancer (PCa) is the most common cancer in the UK, with 1 in 8 affected in their lifetime. However, there are significant race disparities that Black men are more negatively impacted than any other races with their increased risks and worse outcomes. However, they are under-represented in research, and often labelled as ‘hard to reach’ population.AimThe aim of this study was to reach out to a local Black male community using a co-participatory approach, to build up trust and explore their understandings and concerns of the disease.MethodWe recruited 2 community researchers (CRs) from SACMHA (Sheffield African-Caribbean Mental Health Association). Using a co-participatory approach, we worked together throughout the project, including recruitment, planning and facilitating workshops, analysing and presenting data. Two workshops were organised. We introduced an overview of PCa followed by open discussion in several small groups. A rapid qualitative analysis was used to identify key points.ResultsThe engagement was fantastic. We recruited over 30 participants from the Black male community for the first workshop within 4 weeks. A range of topics were raised which were summarised as a Black men’s journey of PCa. The participants were very keen to receive information and have their voice heard. These are facilitating factors in engagement. The males showed concern about their lack of awareness of their high risks of PCa. Personal experiences have also been emphasised throughout the disease journey.ConclusionUsing a co-participatory approach in community research engagement was successful. We shared our knowledge, built trust, and identified research priorities.
Perspective of Dutch healthcare professionals on care for female urinary incontinence: a mixed-methods study
Authors: van Boxtel Jorke , Wessels Nienke , Ruiter Eline , Loohuis Anne , Metting Esther , van der Worp Henk , Blanker Marco 
Published: July 2023

BackgroundUrinary incontinence (UI) impacts patients and society in many ways. There are good treatment options, but healthcare delivery and processes are often suboptimal.AimHealthcare professionals (HCPs) can reveal practical recommendations to improve processes and address challenges in the care of women with UI in the Netherlands.MethodWe conducted an exploratory, sequential, mixed-methods study among HCPs, using outcomes of five focus group sessions (30 HCPs) to inform a subsequent survey. HCPs included GPs, practice assistants (PAs), pelvic physiotherapists (PPTs), and urologists and gynecologists (UGs).ResultsMain themes arising from the five focus group sessions (with six GPs, seven PAs, six (resident) UGs, eight PPTs, and seven PPTs) were ‘identification of UI’, ‘current state of care’, and ‘guiding patients through the healthcare system’. Survey responders (n= 741) included 351 PAs, 124 GPs, 75 PPTs, and 183 UGs. Overall, 72.8% (strongly) agreed that the identification of UI required improvement and 60% confirmed the need for further education. Most HCPs (83.1%) found it useful to offer a patient information leaflet when buying incontinence products but less useful to ask about UI routinely, and most (75%) agreed that a multidisciplinary guideline could improve health care. Interestingly, 86% of PPTs and 21% of UGs advocated referral to a PPT before referral to a specialist, while 87% of PPTs wanted primary care services to offer a UI consultation hour and 36% of the GPs (strongly) disagreed.ConclusionPoor UI identification in primary care and a lack of patient guidance through the healthcare system hamper continence care provision.
Trends in gerontological crime in the context of contemporary challenges and threats
Authors: Vishnevskaya Tatiana 
Published: July 2023

The article reveals the important vector in criminological science by highlighting and studying gerontological crime as a rather autonomous criminogenic social reproduction phenomenon. The author offers the criminological analysis of gerontological crime by means of complex binary approach to its understanding in the view of semantic (critical) and traditional approaches, reveals the main trends of gerontological crime in the Republic of Belarus with regard to its level, dynamics and structure in order to forecast and prevent this type of crime.
Introduction. Gerontological crime, or crime involving deviant behavior of older people in various spheres of human activity, is increasing in the majority of states today. This determines the relevance of its in-depth scientific criminological research in order to develop a modern, effective and comprehensive system of prevention.
Objective. Development of the theoretical and applied aspects of criminological characteristics of gerontological crime.
Research methodology, methods and techniques. The dialectical-materialistic method of scientific cognition, general scientific methods (analysis, synthesis, comparison etc.) as well as special methods (historical, logical, legal, sociological etc.) formed the methodological basis of the research.
Results. The author’s definition of gerontological crime is refined, its main quantitative and qualitative indicators are analysed, trends are revealed and the main determinants are identified, making it possible to carry out preventive activities more effectively.
Scientific novelty. The conclusions and proposals developed in the research significantly enrich the conceptual criminological apparatus, systematise and expand the scientific knowledge on gerontological crime. Fundamentally new in knowledge concerning gerontological crime is that it is examined as an object of social relations that predetermine and motivate the elderly to commit unlawful acts as a manifestation of their negative response to social needs not satisfied by the society. Practical significance. The results of the research are aimed at achieving and maintaining a maximum degree of protection of the interests of individuals, society and the state against gerontological crime and can be used to improve legislation, law enforcement activities of the various subjects of prevention of gerontological crime, which will create conditions for ensuring constitutional legality and the rule of law.
Appropriate N application alleviates growth inhibition of okra (Abelmoschus esculentus L. Moench) caused by fluctuating soil moisture
Authors: Xu Shenghui , Huang Yunxiang , Zhang Renlian , Niu Li , Long Huaiyu 
Published: July 2023

Abstract
Uneven rainfall in the context of global warming can cause soil moisture fluctuation (SMF) that harm crop growth, and it is not yet known whether nitrogen (N) can mitigate the harm caused by the strong SMF. This paper uses okra as a test subject and sets three SMFs of 45–55% FC (W1), 35–65% FC (W2), and 25–75% FC (W3) and three N applications of 0 kg hm− 2 (N0), 110 kg hm− 2 (N1), and 330 kg hm− 2 (N2) to investigate the effects of SMF and N application on physiological and biochemical aspects of okra. The results showed that okra stem diameter, number of leaves, photosynthesis characteristics, antioxidant enzyme activity, and yield were the highest under N1 treatment and considerably greater than under N0 and N2 treatments; stomatal density, antioxidant enzyme activity, malondialdehyde content, and proline content of okra leaves were the highest under W3 treatment, indicating that the plants were subjected to duress under W3 treatment; the N1W1 treatment had the highest okra stem diameter, leaf number, photosynthetic characteristics, and yield, including a yield of 201.3g, which was higher than the lowest treatment (N2W3), a significant increase of 67.8%; W3 was detrimental to okra growth, while the agronomic traits and yields of okra in the N1 treatment were higher than those in the N0 and N1 treatments, indicating that the crop damage caused by W3 could be mitigated by the appropriate amount of N application.
PENGARUH MAJALAH INTERNAL TERHADAP RUTINITAS MINAT BACA KARYAWAN PT KCI JABODETABEK
Authors: Yanuar Irfan , Saripudin Muhammad , Pradana Sumbodo Yama , Fajar Meirawan Rizky 
Published: July 2023

Magazine is one of the writings which contains information that is hotly discussed. To write a magazine, you have to use good and correct grammar. Presenting information that is solutive is indeed one of the indications to attract readers' interest in magazines. The aim of the study was to find out whether employee opinions related to the quality of information and the scope of the news had an effect on employees' routines in reading internal magazines at Pt X. This study used quantitative methods to analyze and process data in the form of numbers using the self-report questionnaire (SRQ) method. Through the SRQ method, individual respondents answered all questions independently using the G-Form platform to process data analysis in statistical tests. Based on research conducted for Internal Magazines on Employee Reading Interest Routines at PT X. namely internal magazines that are informative can influence employees' reading interest routines. And information in internal magazines covering all activities within the company does not affect PT X employees' reading interest. And it can be concluded that employees' routines in reading internal magazines are influenced by the quality of the information. The better the quality of the information, the higher the employee's reading routine.
DISIPLIN PERSPEKTIF DOLET UNARADJAN: SOLUSI ALTERNATIF DALAM MENGUBAH MINDSET DAN PERILAKU PROKRASTINASI AKADEMIK
Authors: Ramadhan Jihan , Maghfiroh Maghfiroh 
Published: July 2023

Abstract: This article examines the discipline offered by Dolet Unaradjan as a solution in improving the academic procrastination mindset and behavior. Where the mindset always leads to the individual's mindset that will produce a behavior in their daily life, the wrong mindset will give birth to the wrong behavior, and vice versa. Mindset often encourages individual human beings to behave according to the desired mindset. One of them is the academic procrastination behavior in individuals, this habit often occurs in every human being. Many studies and studies have discussed this behavior, in which almost every individual human being has this behavior, including us personally who often do the habit of procrastinating a job. This behavior is a symptom that exists in every human individual that starts from the wrong mindset, resulting in the academic procrastination behavior. This article uses library research, which is an effort to use library research to obtain data from library documents such as books, books, magazines, and other documents. Thus, the authors hope that the discipline of Dolet Unaradjan will give a good contribution in solving existing problems. The contribution that the authors have found by conducting a study of this article is that discipline is able to have a strong influence in improving and changing the individual's mindset which ultimately results in a good behavior and attitude, so that academic procrastination behavior can disappear by itself. Which of course is accompanied by personal maturity, be it intellectual, spiritual, and emotional maturity.
Keywords: Discipline, Mindset, Academic Practice
Evaluating Google trends as a primary care research tool
Authors: Fitzgerald Maisie , Saunders Catherine 
Published: July 2023

BackgroundGoogle searches represent a snapshot of the world’s interest in different topics and are reflected by Google Trends (GT). GT has gained recent popularity across various areas of healthcare research of interest to GPs, such as the seasonality of particular physical symptoms and population-level suicide risk. As a real-time data source, GT is appealing to healthcare researchers, but whether it has a place in primary care research remains largely unaddressed.AimTo explore how GT can be used to research topics relating to primary care in order to evaluate its utility as a future research tool and inform primary care researchers and GPs on the uses and implications of GT as a research tool.MethodCollect relative search volume data from GT on topics relating to health behaviours and access to/experiences of primary care in order to evaluate the utility of GT as a tool for future primary care research.ResultsGT was useful for identifying patterns in health behaviours across time but did not offer any useful information on access to/experiences of primary care. Conducting numerous searches on GT identified various limitations, including internet bias and news bias.ConclusionGT is an accessible and vast data source. This study demonstrated the potential for GT to play a role in researching health behaviours. However, this study also revealed key limitations of GT, and caution should be exercised by primary care researchers and GPs when using GT for research.