bluuebunny/crossref_metadata_2025_split huggingface dataset:
Chloroplast Genome Sequencing and Comparative Analysis of Six Medicinal Plants of Polygonatum
Authors: Yao Jinchen , Zheng Zhaohuan , Xu Tao , Wang Duomei , Pu Jingzhe , Zhang Yazhong , Zha Liangping 
Published: January 2025

ABSTRACTThe genus Polygonatum boasts abundant germplasm resources and comprises numerous species. Among these, medicinal plants of this genus, which have a long history, have garnered attention of scholars. This study sequenced and analyzed the chloroplast genomes of six species of Polygonatum medicinal plants (P. zanlanscianense, P. kingianum, P. sibiricum, P. cyrtonema, P. filipes, and P. odoratum, respectively) to explore their interspecific relationships. The sequence length (154, 578–155, 807 bp) and genome structure were conserved among the six Polygonatum species, with a typical tetrad structure. Among the 127–131 genes contained in the genomes, 84–85 are protein‐coding genes, 37–38 are transfer RNA genes, and 6–8 are ribosomal RNA genes. The genomes contained 64–76 simple sequence repeats (SSRs) and 36–62 long repetitive sequences. Codon bias patterns tended to use codons ending in A/T. In 30 types of codons with RSCU > 1, 93.3% ended in A/T of the six species. Twenty‐one highly variable plastid regions were identified in the chloroplast genomes of the six medicinal plants. Furthermore, a phylogenetic analysis encompassing these and 53 other chloroplast genomes of Polygonatum species revealed that P. cyrtonema, P. odoratum, and P. filipes clustered together on one clade, whereas P. kingianum and P. zanlanscianense formed separate clades. Notably, P. sibiricum emerged as a standalone clade, and our phylogenetic tree reinforces the classification of P. sibiricum as forming a monophyly. This study provides a novel basis for intragenus taxonomy and DNA barcoding molecular identification within the genus Polygonatum medicinal plants.
Captivity Reduces Diversity and Shifts Composition of the Great Bustard (Otis tarda dybowskii) Microbiome
Authors: Lu Zhiyuan , Wang Zhucheng , Jia Hexue , Meng Derong , Wu Dayong 
Published: January 2025

ABSTRACTCaptivity offers protection for endangered species, but for bustards, captive individuals face a higher risk of disease and exhibit lower reintroduction success rates. Changes in the diversity of host bacterial and fungal microbiota may be a significant factor influencing reintroduction success. The great bustard (Otis tarda) is a globally recognized endangered bird species. Previous research on the gut microbiota of the great bustard has been limited, hindering effective conservation efforts. Therefore, this study utilized high‐throughput sequencing of the 16S rRNA and Internal Transcribed Spacer (ITS) genes to compare the gut bacterial and fungal microbiota of great bustards in different environments. The results revealed a significant decline in alpha diversity and notable changes in microbial community structure in captive environments. Changes in diet and habitat are likely major factors contributing to these shifts. Consequently, managing rescued wild animals by increasing dietary diversity and exposure to natural environmental reservoirs may enhance the success rate of reintroduction efforts.
The Complete Chloroplast Genome of Erythrina variegata L. (Papilionoideae, Fabaceae)
Authors: Nhat Nam Nguyen , Ngoc Trai Nguyen , Phuong Thuy Nguyen , Quoc Duy Le , Nguyen Tuong Van Pham , Nguyen Tan Tai , Do Hoang Dang Khoa 
Published: January 2025

ABSTRACTErythrina variegata L. 1754, a thorny deciduous tree of Fabaceae, contains various chemical compounds such as alkaloids, flavonoids, and triterpenoids and exhibits anti‐depressant, anti‐inflammatory, and antidiabetic activities. However, genomic data of E. variegata are limited. In this study, the complete chloroplast genome of E. variegata was sequenced and characterized using Illumina sequencing platform. The chloroplast genome of E. variegata was 152,351 bp in length and consisted of a large single copy (82,907 bp), a small single copy (26,309 bp), and two inverted repeat regions (16,826 bp). There were 79 protein‐coding genes, 30 transfer RNA genes, and four ribosomal RNA genes. Comparative analysis revealed high conservation of chloroplast genomes among Erythrina species regarding genome size, structure, and gene content. The phylogenetic study also indicated a close relationship between E. variagata and E. sanwicensis. This study provides initial plastome data for further genomic studies examining E. variegata and related species in Fabaceae.
Sacubitril/valsartan versus valsartan initiation in patients naïve to renin–angiotensin system inhibitors: Insights from PARAGLIDE‐HF
Authors: Nouhravesh Nina , Gunn Alexander H. , Cyr Derek , Hernandez Adrian F. , Morrow David A. , Velazquez Eric J. , Ward Jonathan H. , Sarwat Samiha , Sharma Kavita , Williamson Kristin M. , Starling Randall C. , Lepage Serge , Zieroth Shelley , Solomon Scott D. , Mentz Robert J. 
Published: January 2025

AbstractAimsWhether prior treatment with angiotensin‐converting enzyme inhibitors (ACEi) or angiotensin receptor blockers (ARBs) modifies efficacy and safety of sacubitril/valsartan (Sac/Val) in patients with heart failure (HF) and ejection fraction (EF) >40% is unclear, thus Sac/Val according to ACEi/ARB status at baseline was assessed.Methods and resultsThis was a pre‐specified analysis of Prospective comparison of ARNI with ARB Given following stabiLization In DEcompensated HFpEF (PARAGLIDE‐HF), a double‐blind, randomized controlled trial of Sac/Val versus valsartan, categorizing patients according to baseline ACEi/ARB status. The primary endpoint was time‐averaged proportional change in N‐terminal pro‐B‐type natriuretic peptide (NT‐proBNP) from baseline through weeks 4 and 8. Secondary analyses included a win‐ratio analysis of the hierarchical outcome of (i) cardiovascular death; (ii) HF hospitalizations; (iii) urgent HF visits; and (iv) time‐averaged proportional change in NT‐proBNP from baseline to weeks 4 and 8, in addition to safety outcomes. Among 466 patients, 107 (23%) were ACEi/ARB naïve at the time of randomization. NT‐proBNP favoured Sac/Val irrespective of ACEi/ARB status (naïve: 0.76, 95% confidence interval [CI] 0.51–1.13; users: 0.88, 95% CI 0.74–1.05; pinteraction = 0.52). The win ratio of the hierarchical outcome was 1.13 (95% CI 0.86–1.49) for ACEi/ARB users and 1.38 (95% CI 0.81–2.37) for ACEi/ARB naïve (pinteraction = 0.51). Safety endpoints showed non‐significant interactions by ACEi/ARB status, with odds ratio of 1.79 (95% CI 0.68–4.72) and 0.71 (95% CI 0.29–1.78) for symptomatic hypotension and worsening renal function, respectively for ACEi/ARB naïve.ConclusionIn HF with EF >40% stabilized after worsening HF, safety and efficacy were similar irrespective of ACEi/ARB status at baseline, supporting early initiation irrespective of prior ACEi/ARB use.
Chemical and Electrochemical Oxidation of Diferrocenyltelluride
Authors: Stoian Corina , Schmidt Nils , Puylaert Pim , Linden Mathias H. , Bernhard Linden H. , Beckmann Jens 
Published: January 2025

AbstractThe halogenation of diferrocenyltelluride, Fc2Te, with XeF2, PhICl2 and I2 afforded the diferrocenyltellurium dihalides Fc2TeX2 (X=F, Cl, I). Halide exchange of Fc2TeCl2 with KBr yielded Fc2TeBr2. The oxidation of Fc2Te with NO[SbF6] and K[B(C6F5)4] provided the triferrocenyltelluronium salt [Fc3Te][B(C6F5)4] and the dinuclear nitrosyl iron complex Fe2(μ‐TeFc)2(NO)4. The new compounds were comprehensively characterized by multinuclear NMR spectroscopy (125Te, 19F, 13C, 1H), UV/vis absorption spectroscopy, cyclic voltammetry, LIFDI‐TOF mass spectrometry and single crystal X‐ray diffraction.
Interconnected Microgrids Load‐Frequency Control Using Stage‐by‐Stage Optimized TIDA+1 Error Signal Regulator
Authors: Shayeghi Hossein , Rahnama Alireza , Bizon Nicu , Szumny Antoni 
Published: January 2025

ABSTRACTLoad‐frequency control (LFC) is essential for maintaining system stability and ensuring high power quality in microgrids (MGs), particularly those heavily reliant on renewable energy sources (RES) and operating independently of the main grid. This paper introduces a novel control strategy aimed at improving LFC performance in interconnected MGs by correcting the error signal. The proposed controller, denoted as TIDA+1, combines tilt, integrator, derivative, and acceleration operators in a parallel configuration to refine the incoming error signal. The controller parameters are optimized using a modified particle swarm optimization (PSO) algorithm with nonlinear time‐varying acceleration coefficients (NTVAC). The controller's effectiveness is validated through four distinct scenarios, including sudden load variations, system modeling uncertainties, fluctuations in RES outputs, and the impact of nonlinearities. Additionally, a lab‐scale evaluation of the controller has been conducted to further assess its practical applicability. Comparative results demonstrate that the TIDA+1 controller outperforms traditional controllers such as PID and FOPID, especially under complex operational conditions. The study highlights the TIDA+1 controller as a robust and viable solution for LFC in MGs, with potential for future scalability and application in larger systems.
Thermodynamic Performance of a Novel Compressed Carbon Dioxide Energy Storage System Based on Guanidinium Sulfate (Gua2SO)4) Adsorption
Authors: Liu Jiaxin , Liu Zhan 
Published: January 2025

ABSTRACTIn recent years, energy storage technology has developed rapidly with the aim to promote the development of renewable energy sources and establish a green and sustainable energy structure. A novel compressed CO2 energy storage system based on Gua2SO4 adsorption is proposed in this study. Gua2SO4 has low sorption enthalpy and mild physical conditions in the adsorbent and desorption process, which are very beneficial for reducing the design difficulty of low‐pressure gas storage devices and improving the system performance. The energy and exergy analysis models are conducted after establishing several assumptions. The round trip efficiency, energy density, CO2 capture unit volume and liquid CO2 tank volume are 68.8%, 12.6 kWh/m3, 44,208 m3 and 19,235 m3 under design conditions, respectively. In addition, according to the exergy analysis, the liquefaction unit and compression unit have the highest exergy destructions. The monotonic increase in high‐pressure cooler end temperature difference and ambient temperature will cause the system efficiency to show a trend of first increasing and then decreasing. The liquid CO2 tank temperature and throttling pressure have a trade‐off relationship on system performance. It should be noted that when the throttling pressure is below a certain value and the liquid CO2 tank temperature is above a certain value, the system efficiency will sharply decrease. Moreover, increasing compressor and turbine efficiency has a conducive effect on improving system efficiency while the growth of heat exchanger pinch temperature and pressure loss rate have a negative influence to that.
New Laboratory Results on Ignition and Propagation in Live Vegetation Paving the Road to a Semi‐Empirical Model
Authors: Guillaume Bruno , Ganteaume Anne , Majeri Malek , Fayad Jacky , El Houssami Mohamad , Pizzo Yannick , Porterie Bernard 
Published: January 2025

ABSTRACTWildfires arriving at a brush‐cleared vegetation near vulnerable assets in the Wildland‐Urban Interface (WUI), experience significantly reduced fire propagation, intensity, and associated risks. However, assessing the effectiveness of fuel reduction on fire behavior remains challenging due to limited understanding of ignition and propagation mechanisms in live vegetation. To fill this gap, a simplified approach was designed, focusing more on the combination of physical modeling with new empirical data rather than providing new insight in the physical process modeling. Burning experiments were conducted on cypress trees at two scales of live vegetation, the “laboratory scale” and the “real scale,” to gather data on fire behavior in cypress canopies with varying fuel moisture content (FMC) and bulk density (BD), using two ignition methods. A semi‐empirical model, based on the physical model Fire Dynamic Simulator was developed, using the “laboratory scale” data as inputs, while the data recorded at “real scale” were used to validate the model. Laboratory‐scale experiments showed consistent results when ignition was initiated by flame contact. In contrast, indirect radiant heat ignition was highly variable due to the influence of gaps between leaves. At the real scale, BD had a significant impact on fire behavior. The model evaluation showed it could simulate fire auto‐propagation in live vegetation much more precisely compared to current physical models, leveraging the precise fire behavior data obtained at the laboratory scale.
Incorporating the Malnutrition Screening Tool and the Malnutrition Universal Screening Tool in Rehabilitation Practice: Comparison With the Nutrition Risk Screening 2002
Authors: Tel Adigüzel Kübra , Çalişkan Hatice Aybüke , Işik Fatma Berna , Çaybaşi Erdoğan Hilal , Akşit Sena , Mansiz Suna , Adigüzel Emre , Yaşar Evren 
Published: January 2025

ABSTRACTTo demonstrate the prevalence of malnutrition risk in a specific rehabilitation setting. The secondary aim of the study was to compare Malnutrition Screening Tool (MST) and Malnutrition Universal Screening Tool (MUST) with Nutritional Risk Screening‐2002 (NRS‐2002). Patients diagnosed with stroke, anoxic brain injury, spinal cord injury, multiple sclerosis, arthritis, neuromuscular diseases, Parkinson's disease, and lymphedema who were admitted to a rehabilitation hospital were included. NRS‐2002, MST, and MUST were used to assess malnutrition risk. Body weight (kg), height (cm), and mid upper arm circumference (cm) were measured. Twenty‐four hours dietary records were obtained. Routine blood test results were recorded from patient files. Five hundred sixteen patients with a mean age of 54.3 ± 18.0 years were included. The most prominent diagnoses were stroke and spinal cord injury. According to NRS‐2002, 71.7% (n = 370) of the patients were at low risk, but 28.3% (n = 146) of the patients were at high risk. Comparisons between NRS‐2002 and MST showed that these two scales have similar results at classifying patients for malnutrition risk (p = 0.154). Comparison between NRS‐2002 and MUST showed significant differences (p < 0.001). Both sensitivity and specificity of MST were above 80.0%. Sensitivity of MUST was 78.1% and specificity was 88.1%. Approximately one‐third of the patients were at risk of malnutrition. Specificity and sensitivity of MST and MUST were as high as routinely used scale NRS‐2002, and therefore it can be supposed that, considering the diagnostic groups of the patients, MST and MUST are useful in rehabilitation practice.
Balancing Data Quality and Bias: Investigating Functional Connectivity Exclusions in the Adolescent Brain Cognitive Development℠ (ABCD Study) Across Quality Control Pathways
Authors: Peverill Matthew , Russell Justin D. , Keding Taylor J. , Rich Hailey M. , Halvorson Max A. , King Kevin M. , Birn Rasmus M. , Herringa Ryan J. 
Published: January 2025

ABSTRACTAnalysis of resting state fMRI (rs‐fMRI) typically excludes images substantially degraded by subject motion. However, data quality, including degree of motion, relates to a broad set of participant characteristics, particularly in pediatric neuroimaging. Consequently, when planning quality control (QC) procedures researchers must balance data quality concerns against the possibility of biasing results by eliminating data. In order to explore how researcher QC decisions might bias rs‐fMRI findings and inform future research design, we investigated how a broad spectrum of participant characteristics in the Adolescent Brain and Cognitive Development (ABCD) study were related to participant inclusion/exclusion across versions of the dataset (the ABCD Community Collection and ABCD Release 4) and QC choices (specifically, motion scrubbing thresholds). Across all these conditions, we found that the odds of a participant's exclusion related to a broad spectrum of behavioral, demographic, and health‐related variables, with the consequence that rs‐fMRI analyses using these variables are likely to produce biased results. Consequently, we recommend that missing data be formally accounted for when analyzing rs‐fMRI data and interpreting results. Our findings demonstrate the urgent need for better data acquisition and analysis techniques which minimize the impact of motion on data quality. Additionally, we strongly recommend including detailed information about quality control in open datasets such as ABCD.
Effects of Department Type and Disability Status on Medical School Faculty Wellbeing
Authors: Abulela Mohammed A. A. , Schowengerdt Bethany , Dorr Heather , Termuhlen Amanda , Krohn Kristina , Violato Claudio 
Published: January 2025

ABSTRACTPurposeFaculty wellbeing impacts student learning and is a priority among medical schools, especially as a counterbalance to growing burnout. Previous researchers found differences in burnout by sex and race among clinicians, but not for faculty with disabilities. Accordingly, the purpose was to test the association between faculty's wellbeing, burnout, and control over workload and investigate differences in wellbeing attributed to department type and ability status.MethodThe authors developed and administered a comprehensive wellbeing survey to University of Minnesota Medical School faculty, of whom 703 provided complete responses. The authors conducted two‐way ANOVA followed by a post hoc analysis to test for differences in faculty wellbeing domains due to department type (basic sciences, nonsurgical, surgical, and two large departments of Medicine and Pediatrics) and disability status (yes, no). The authors also fitted a two‐way ordinal model since burnout frequency and control over workload were assessed by one ordinal item each.ResultsWellbeing domains were positively correlated with control over workload but negatively associated with burnout. Faculty with disabilities reported less support from their work environment and meeting of their basic needs. Department type had a statistically significant impact on faculty's sense of basic needs, respect, and contribution. Multiple comparisons revealed faculty in basic sciences departments had higher scores within basic needs compared to the departments of Medicine, Pediatrics, and surgical departments, who reported lower levels of respect as well. Results revealed department type and disability status affected the frequency of burnout, as faculty in basic sciences departments reported lower levels of burnout compared to other departments.ConclusionsResults support disaggregating wellbeing by department and ability status for targeted interventions due to differences‐ notably among faculty with disabilities and surgical departments‐ in their assessment of basic needs, work environment, respect, and contribution. Results suggest revisiting interventions in these domains to account for lower reported wellbeing.
The Influence of Sanitary Infrastructure on Event Nutrient Dynamics in a Headwater Catchment
Authors: Spill Caroline , Ditzel Lukas , Gassmann Matthias 
Published: January 2025

ABSTRACTMany low‐order streams are recipients of effluents from wastewater treatment plants (WWTPs) and combined sewer overflows (CSOs). Not only do these facilities have to meet fewer requirements compared to their bigger counterparts in more densely populated areas, but they also discharge into smaller, more vulnerable streams, with low dilution potential. Although these local point sources can dictate the local water quality and quantity dynamics, they are barely monitored and often not included in catchment‐wide analyses. In this case study we measured stream water quantity and quality in a first‐order, point source‐influenced stream. We have specifically addressed point sources in our monitoring programme, which included the installation of a low‐budget probe at the CSO outlet to monitor overflow timing. By clustering hysteresis loops and using Principal Component Analysis, we were able to identify hydro‐meteorological drivers and reveal seasonal patterns of discharge and nutrient export dynamics. Mobilisation of nitrate from agricultural sources clearly dominated event dynamics during periods with high soil moisture, while point source dynamics overlaid catchment responses during the rest of the time. Thus, the dilution potential of the stream was found to be a controlling factor for water quality dynamics. Groundwater infiltration into the sewer system probably increased the risk of CSO discharges, especially in winter and spring. In summer, CSO spillages occurred as a result of high‐intensity rainfall. These events were related to an increase of turbidity and a mobilisation of particulate phosphorus. With our novel approach, including urban point sources in our monitoring setup, we were able to show the close relationship between sanitary infrastructure in rural areas and stream nutrient dynamics. Including point sources more closely into monitoring and analysis is essential to improve the process understanding.
Mycobacterial PstP impairs host RNA alternative splicing by dephosphorylation of spliceosome RBMX at S189
Authors: Liu Tianxian , Xu Jun‐Yu , Zhao Lei , Fan Yameng , Xie Shuyu , Ma Ke , Zhou Ying , Tan Minjia , Ye Bang‐Ce 
Published: January 2025

AbstractMycobacterium tuberculosis (Mtb) infection significantly alters host cellular signaling and protein functions, facilitating immune evasion and intracellular survival. However, the molecular mechanisms underlying these interactions remain incompletely characterized. Here, we employed a multi‐omics strategy, including proteomics, phosphoproteomics, transcriptomics and interactomics, to investigate the impact of Mtb infection on host cellular processes. Our study revealed that mycobacteria modulate RNA alternative splicing in host cells by reducing the phosphorylation levels within the spliceosome complex. We identified the serine/threonine protein phosphatase (PstP) as a key effector, dephosphorylating the spliceosome RNA‐binding motif protein (RBMX) at the serine 189 site (S189). This modification influences the alternative splicing of PLA2G7, which encodes platelet‐activating factor acetylhydrolase, resulting an increase in the mRNA levels of a transcript containing exon9 (PLA2G7‐exon9+). Importantly, PLA2G7 isoform encoded by PLA2G7‐exon9+, in contrast to the isoform lacking exon9, acquires the ability to potentiate inflammatory responses. Collectively, our findings not only provide a comprehensive view of Mtb‐induced host regulatory networks but also elucidate a role for PstP in controlling a critical mediator of alterative splicing during infection.
Confronting the post‐truth phenomenon in literacy education: The need for a critical media epistemology
Authors: Lathrop Benjamin N. 
Published: January 2025

AbstractThe 2016 presidential election marked the beginning of a so‐called “post‐truth era” in which mis‐ and disinformation have contributed to political polarization and violence, the acceleration of global warming, and thousands of preventable deaths. In this paper, I draw on the results of a 2‐week, practitioner inquiry‐informed case study in an 11th‐grade English language arts (ELA) classroom situated in a small, conservative Midwest town to explore the affordances of integrating two theoretical frameworks, one rooted in media literacy and one in epistemic cognition, in planning and implementing a unit designed to support students' critical engagement with digital media texts related to climate change. The findings point to the need for a new framework that draws on insights from both sociocultural and cognitive approaches to literacy. Implications for classroom practice include the importance of teaching students how to evaluate their positionality using platforms they already engage, creating authentic epistemic environments, leading students in explorations of different ways of knowing, helping them understand knowledge systems and processes, and promoting epistemic virtues and autonomy.