bluuebunny/crossref_metadata_2025_split huggingface dataset:
Methanotrophic potential of Dutch canal wall biofilms is driven by Methylomonadaceae
Authors: Pelsma Koen A J , Verhagen Daniël A M , Dean Joshua F , Jetten Mike S M , Welte Cornelia U 
Published: September 2023

Abstract
Global urbanization of waterways over the past millennium has influenced microbial communities in these aquatic ecosystems. Increased nutrient inputs have turned most urban waters into net sources of the greenhouse gases carbon dioxide (CO2) and methane (CH4). Here, canal walls of five Dutch cities were studied for their biofilm CH4 oxidation potential, alongside field observations of water chemistry, and CO2 and CH4 emissions. Three cities showed canal wall biofilms with relatively high biological CH4 oxidation potential up to 0.48 mmol gDW−1 d−1, whereas the other two cities showed no oxidation potential. Salinity was identified as the main driver of biofilm bacterial community composition. Crenothrix and Methyloglobulus methanotrophs were observed in CH4-oxidizing biofilms. We show that microbial oxidation in canal biofilms is widespread and is likely driven by the same taxa found across cities with distinctly different canal water chemistry. The oxidation potential of the biofilms was not correlated with the amount of CH4 emitted but was related to the presence or absence of methanotrophs in the biofilms. This was controlled by whether there was enough CH4 present to sustain a methanotrophic community. These results demonstrate that canal wall biofilms can directly contribute to the mitigation of greenhouse gases from urban canals.
Sociosexual Exposure Has Opposing Effects on Male and Female Actuarial Senescence in the Fruit Fly Drosophila melanogaster
Authors: Rostant Wayne G , Mason Janet S , West Nicholas , Maklakov Alexei A , Chapman Tracey 
Published: September 2023

Abstract
Males and females rarely express the same length of life. Here, we studied how sociosexual exposure shapes male and female age-specific mortality rates in Drosophila melanogaster. We maintained focal females and males within large, replicated cohorts throughout life with individuals of the same or opposite sex. Consistent with previous works, we found that females kept throughout their lives with males had only half the lifespan of those maintained throughout life at the same density in same-sex cohorts. In contrast, only a small lifespan decrease was observed in the corresponding male treatments and the reduction in male lifespan following exposure throughout life to other males or females was similar. Deconvolution of underlying aging parameters revealed that changes in lifespan were underpinned by opposing effects on actuarial aging in males versus females. Exposure to the opposite or same sex increased initial mortality rate in both sexes. However, in females, increasing exposure to males increased the rate of aging, while increasing exposure to females actually decreased it. The effects were in the opposite direction in males and were much smaller in magnitude. Overall, the findings were consistent with reports suggesting that exposure to the same versus opposite sex can affect survival differently in males and females. However, they also reveal a new insight—that overall lifespan can be underpinned by key differences in actuarial senescence in each sex. The findings suggest that responses to same or opposite sex exposure may have fundamentally and qualitatively different physiological consequences for health in males and females.
Global empirical models for infrasonic celerity and backazimuth
Authors: Nippress Alexandra , Green David N 
Published: September 2023

SUMMARY
Global empirical models for infrasound signal celerity (where celerity is defined as the epicentral distance divided by the total traveltime) and backazimuth deviation (the difference between the measured and predicted backazimuth assuming great circle propagation), can be used for the association of infrasound automatic detections, for event location and for the validation of acoustic propagation simulations. Using software developed to consistently analyse a global ground truth database, we observe 296 detections in the 0.32–1.28 Hz passband; predominantly stratospheric arrivals, with a smaller number of tropospheric and thermospheric arrivals, in agreement with previous studies. We develop an updated global celerity-range model and introduce a global backazimuth deviation model. These new models suggest that the variation in backazimuth deviation is not range-dependent; 93 per cent of the 296 detections studied have a deviation magnitude ≤5°. The maximum deviation observed is 8.9°. The variation in celerity, however, is range-dependent. An exponential range-dependent celerity model with bounds calculated using a quantile regression fit to the traveltime residuals is determined. This traveltime residual model, with uncertainties that increase with range, produces bounds on the celerity consistent with both the observations and current understanding of infrasound propagation. The traveltime residual model derived celerity bounds are not symmetric around the celerity model, which further reflects the physical processes. At long ranges (&gt;3000 km), maximum peak-to-trough amplitude arrivals are not observed with celerities &lt;280 ms−1, but even at long ranges, we occasionally observe celerities (&gt;320 −1) more usually consistent with tropospheric arrivals.
Development of a novel target-based cell assay, reporter of the activity of Mycobacterium tuberculosis protein-O-mannosyltransferase
Authors: Géraud Nicolas , Falcou Camille , Parra Julien , Froment Carine , Rengel David , Burlet-Schiltz Odile , Marcoux Julien , Nigou Jérôme , Rivière Michel , Fabre Emeline 
Published: September 2023

Abstract
The Protein-O-mannosyltransferase is crucial for the virulence of Mycobacterium tuberculosis, the etiological agent of tuberculosis. This enzyme, called MtPMT (Rv1002c), is responsible for the post-translational O-mannosylation of mycobacterial proteins. It catalyzes the transfer of a single mannose residue from a polyprenol phospho-mannosyl lipidic donor to the hydroxyl groups of selected Ser/Thr residues in acceptor proteins during their translocation across the membrane. Previously, we provided evidence that the loss of MtPMT activity causes the absence of mannoproteins in Mycobacterium tuberculosis, severely impacting its intracellular growth, as well as a strong attenuation of its pathogenicity in immunocompromised mice. Therefore, it is of interest to develop specific inhibitors of this enzyme to better understand mycobacterial infectious diseases. Here we report the development of a “target-based” phenotypic assay for this enzyme, assessing its O-mannosyltransferase activity in bacteria, in the non-pathogenic Mycobacterium smegmatis strain. Robustness of the quantitative contribution of this assay was evaluated by intact protein mass spectrometry, using a panel of control strains, overexpressing the MtPMT gene, carrying different key point-mutations. Then, screening of a limited library of 30 compounds rationally chosen allowed us to identify 2 compounds containing pyrrole analogous rings, as significant inhibitors of MtPMT activity, affecting neither the growth of the mycobacterium nor its secretion of mannoproteins. These molecular cores could therefore serve as scaffold for the design of new pharmaceutical agents that could improve treatment of mycobacterial diseases. We report here the implementation of a miniaturized phenotypic activity assay for a glycosyltransferase of the C superfamily.
Horizontally integrated health systems and neonatal intensive care unit (NICU) care for very preterm infants
Authors: Rogowski Jeannette , Greenberg Lucy , Edwards Erika , Ehret Danielle , Horbar Jeffrey 
Published: September 2023

Abstract
Hospital care has consolidated rapidly into health systems in the United States. Infants born very preterm are among the most vulnerable pediatric populations, accounting for the majority of infant deaths each year. The pediatric health care delivery system for infants is unique as the birth hospitalization includes 2 patients, the mother and the infant. Further, regionalization goals for infants who are born preterm require care to be provided at neonatal intensive care units (NICUs) with the capacity to treat them. National patient-level data from the Vermont Oxford Network demonstrates that most very preterm infants were born in a horizontally integrated, multi-hospital system (84%), and they tended to remain in the system for their entire hospitalization, including for risk-appropriate NICU care. Half of the infants were cared for in large systems with more than 10 hospitals that were disproportionately cross-market systems. With high transfer rates between hospitals (21%) it will be important to determine the implications of consolidation for the quality of care and patient-centeredness for families. The care for very preterm infants is important from a policy perspective as hospitalized newborn infants account for 21% of hospitalizations in Medicaid each year and 10% of aggregate hospital costs.
The Symbolic Management of Protest: Conceptualization, Typology, and Patterns in Mexican Protest
Authors: Sullivan Heather 
Published: September 2023

AbstractMany protests are met with symbolic responses instead of, or in addition to, concrete actions, yet concessions and repression receive more scholarly attention than any other protest outcome. This article develops a typology of symbolic protest management, the performative or rhetorical responses that are not only directed at a protesting group, but also are intended to influence the public’s understanding of protest events. The typology is structured around two questions: Does the state response treat protests or protesters as legitimate? And does the state respond in a way that signals a resolution of the conflict? Then, using original data on protest in Mexico, the article explores patterns of symbolic response to protest in Mexico.
Across mountains and ocean: species delimitation and historical connectivity in Holarctic and Arctic-Alpine wolf spiders (Lycosidae, Pardosa)
Authors: Ivanov Vladislav , Blagoev Gergin , Danflous Samuel , Gajdoš Peter , Høye Toke Thomas , Lee Kyung Min , Marusik Yuri , Mielec Cecilie Lohse , Muster Christoph , Pétillon Julien , Spelda Jörg , Mutanen Marko 
Published: September 2023

Abstract
Holarctic species offer great opportunities to study biogeography, phylogenetics, taxonomy, and local adaptation. Species that are considered conspecific between the Palearctic and the Nearctic realms are often split into 2 or more species when denser sampling and molecular markers are applied. Similar in complexity but at a finer geographical scale are species groups having Arctic-Alpine distributions where lineages have complicated demographic histories due to glacial dynamics. In both cases, allopatric speciation might not result in fast differentiation of morphological characters if environmental conditions in isolated areas are similar and the main driver of variability is genetic drift. Here, we study the Holarctic Pardosa hyperborea (Thorell, 1872) and its closest European relatives to assess their taxonomic status and patterns of genetic variability. Based on DNA barcodes and genomic data from double-digest restriction site associated sequencing, we propose that the North American populations should be regarded as a distinct species (P. luteola Emerton,1894, stat. resurr.), possibly consisting of several independent lineages. With the help of D-statistics, population genetic simulations and phylogenetic networks analysis, we demonstrate historical introgression among European species of the group and a likely explanation for shared DNA barcodes among allopatric and fully differentiated species. Our study exposes a promising model for studying speciation processes and demographic history in parallel on both sides of the Atlantic Ocean and demonstrates the usefulness of genomic tools in elucidating the taxonomy and biogeography of taxa across broad geographic scales.
Neurological burden and outcomes of excessive β-lactam serum concentrations of critically ill septic patients: a prospective cohort study
Authors: Zerbib Yoann , Gaulin Clement , Bodeau Sandra , Batteux Benjamin , Lemaire-Hurtel Anne-Sophie , Maizel Julien , Kontar Loay , Bennis Youssef 
Published: September 2023

Abstract

Background
Therapeutic drug monitoring (TDM) contributes to optimizing exposure to β-lactam antibiotics. However, how excessive exposure to β-lactams can increase the burden of care of critically ill patients is unclear.

Patients and methods
In a prospective cohort study, we examined whether excessive β-lactam serum concentrations contribute to neurological deterioration and the associated complications of adult septic patients without recent history of neurological disease treated with β-lactams in a medical ICU. Excessive β-lactam concentrations were defined as serum concentrations that exceeded the upper limit of the therapeutic range recommended by the French Societies of Pharmacology and Therapeutics (SFPT) and Anesthesia and Intensive Care Medicine (SFAR). Neurological deterioration was defined as an increase in the neurological Sequential Organ Failure Assessment score (nSOFA) of ≥1 between the day of starting treatment at admission and the day of TDM performed 2 days after treatment initiation.

Results
We included 119 patients [median age: 65 years; males: 78 (65.5%)] admitted for acute respiratory distress [59 (49.6%)] or septic shock [25 (21%)]. In adjusted logistic regression analysis, an excessive β-lactam serum concentration was associated with neurological deterioration [OR (95% CI): 10.38 (3.23–33.35), P &lt; 0.0001]. Furthermore, in adjusted linear regression analysis, an excessive β-lactam serum concentration was associated with longer time to discharge alive (β=0.346, P = 0.0007) and, among mechanically ventilated patients discharged alive, with longer time to extubation following the withdrawal of sedation (β=0.248, P = 0.0030).

Conclusions
These results suggest that excessive exposure to β-lactams could complicate the management of septic patients in the ICU and confirm the clinical relevance of the upper concentration limits recommended for dose reduction.
The implications of large home range size in a solitary felid, the Leopard (Panthera pardus)
Authors: Parker Daniel M , Nams Vilis O , Balme Guy A , Begg Colleen , Begg Keith , Bidner Laura , Bockmuehl Dirk , Cozzi Gabriele , du Preez Byron , Fattebert Julien , Golabek Krystyna , Grant Tanith , Hayward Matt W , Houser Ann-Marie , Hunter Luke T B , Isbell Lynne A , Jenny David , Loveridge Andrew J , Macdonald David W , Mann Gareth K H , Maputla Nakedi , Marker Laurie , Martins Quinton E , Maruping-Mzileni Nkabeng , Melzheimer Joerg , Menges Vera , Nyoni Phumuzile , O’Brien John , Owen Cailey , Parker Tim , Pitman Ross , Power R John , Slotow Rob , Stein Andrew , Steyn Villiers , Stratford Ken , Swanepoel Lourens H , Vanak Abi , Van Vuuren Rudi , Wachter Bettine , Weise Florian , Wilmers Chris C 
Published: September 2023

Abstract
The size of the home range of a mammal is affected by numerous factors. However, in the normally solitary, but polygynous, Leopard (Panthera pardus), home range size and maintenance is complicated by their transitory social grouping behavior, which is dependent on life history stage and/or reproductive status. In addition, the necessity to avoid competition with conspecifics and other large predators (including humans) also impacts upon home range size. We used movement data from 31 sites across Africa, comprising 147 individuals (67 males and 80 females) to estimate the home range sizes of leopards. We found that leopards with larger home ranges, and in areas with more vegetation, spent longer being active and generally traveled faster, and in straighter lines, than leopards with smaller home ranges. We suggest that a combination of bottom-up (i.e., preferred prey availability), top-down (i.e., competition with conspecifics), and reproductive (i.e., access to mates) factors likely drive the variability in Leopard home range sizes across Africa. However, the maintenance of a large home range is energetically expensive for leopards, likely resulting in a complex evolutionary trade-off between the satisfaction of basic requirements and preventing potentially dangerous encounters with conspecifics, other predators, and people.
Clinical and molecular characteristics of early-onset vs average-onset esophagogastric cancer
Authors: Lumish Melissa A , Walch Henry , Maron Steven B , Chatila Walid , Kemel Yelena , Maio Anna , Ku Geoffrey Y , Ilson David H , Won Elizabeth , Li Jia , Joshi Smita S , Gu Ping , Schattner Mark A , Laszkowska Monika , Gerdes Hans , Jones David R , Sihag Smita , Coit Daniel G , Tang Laura H , Strong Vivian E , Molena Daniela , Stadler Zsofia K , Schultz Nikolaus , Janjigian Yelena Y , Cercek Andrea 
Published: September 2023

Abstract

Background
The rate of esophagogastric cancer is rising among individuals under 50 years of age. It remains unknown whether early-onset esophagogastric cancer represents a unique entity. This study investigated the clinical and molecular characteristics of early-onset and average-onset esophagogastric cancer .

Methods
We reviewed the Memorial Sloan Kettering Cancer Center gastric, esophageal, and gastroesophageal junction cancer database. Associations between baseline characteristics and tumor and germline molecular alterations were compared between those with early-onset and average-onset esophagogastric cancer using Fisher exact tests and the Benjamini-Hochberg method for multiple-hypothesis correction.

Results
We included 1123 patients with early-onset esophagogastric cancer (n = 219; median age = 43 years [range = 18-49 years]) and average-onset esophagogastric cancer (n = 904; median age = 67 years [range = 50-94 years]) treated between 2005 and 2018. The early-onset group had more women (39% vs 28%, P = .002). Patients with early-onset esophagogastric cancer were more likely to have a gastric primary site (64% vs 44%, P &lt; .0001). The signet ring cell and/or diffuse type was 3 times more common in the early-onset esophagogastric cancer group (31% vs 9%, P &lt; .0001). Early-onsite tumors were more frequently genomically stable (31% vs 18%, P = .0002) and unlikely to be microsatellite instability high (2% vs 7%, P = .003). After restricting to adenocarcinoma and signet ring cell and/or diffuse type carcinomas, we observed no difference in stage (P = .40) or overall survival from stage IV diagnosis (median = 22.7 vs 22.1 months, P = .78).

Conclusions
Our study supported a preponderance of gastric primary disease sites, signet ring histology, and genomically stable molecular subtypes in early-onset esophagogastric cancer. Our findings highlight the need for further research to define the underlying pathogenesis and strategies for early detection and prevention.
The role of the philosopher of education in the task of decoloniality
Authors: Azada-Palacios Rowena 
Published: September 2023

Abstract
This paper explores the meaning of ‘decolonization’ in relation to the school curriculum and the role of the philosopher of education in this task. Taking the Philippines as an example, this paper illustrates how coloniality has underpinned not only school curricula, but also entire systems of formal education in the post-colony. Following from this, it argues that decolonization in education must transcend the diversification of curricula and aim at a broader vision of justice. Drawing from the author’s own attempts to reimagine the teaching of national identity, the paper proposes that philosophers of education who wish to participate in the work of decoloniality view their contribution as the three-fold task of historical critique, conceptual retrieval, and creative reimagination.
Looking back to look forward—the history of VAD laws in Australia and future law reform in the Australian territories
Authors: Braun Kerstin 
Published: September 2023

Abstract
Taking one’s own life or attempting to do so has long been decriminalised in Australia. Aiding, counselling, or inciting another person to kill him or herself, however, remains a criminal offence. Yet, all six Australian States have now introduced laws allowing assistance in dying under certain circumstances. This article traces the recent history of Voluntary Assisted Dying (VAD) laws in Australia. It examines the introduction of the world’s first assisted dying legislation in the Northern Territory in 1995 followed by the Federal Government’s 1997 deprivation of the Territories’ power to legislate on assisted dying invalidating said law. It further considers the fifty-seven failed Bills attempting to achieve law reform in this context in Australian jurisdictions between 1993 and 2017 with a view to identifying what factors may have contributed to the continuing lack of success. This article then outlines the rapid introduction of VAD laws in all six Australian States decriminalising VAD over the span of only 5 years. It ponders what may have changed to bring about this law reform. This article closes by contemplating potential future law reform in the Australian Territories, which have been reinstated with jurisdiction to legislate on VAD in December 2022.
Four new compact triply eclipsing triples found with Gaia and TESS
Authors: Czavalinga Donát R , Borkovits Tamás , Mitnyan Tibor , Rappaport Saul A , Pál András 
Published: September 2023

ABSTRACT
This paper presents a comprehensive analysis of four triply eclipsing triple star systems, namely TIC 88206187, TIC 14839347, TIC 298714297, and TIC 66893949. The four systems with third-body eclipses were found in the TESS light curves from among a sample of ∼400 matches between known eclipsing binaries and the Gaia DR3 Non-Single Star solution data base. We combined photometric light curves, eclipse timing variations, archival spectral energy distributions, and theoretical evolution tracks in a robust photodynamical analysis to determine the orbital and system parameters. The triples have outer periods of 52.9 d, 85.5 d, 117 d, and 471 d, respectively. All dozen stars have masses ≲ 2.6 M⊙. The systems are quite flat with mutual inclination angles between the inner and outer orbital planes that are all ≲ 4°. The outer mass ratios (q ≡ M3/Mbin) range from 0.39 to 0.76, consistent with our earlier collection of compact triply eclipsing triples. TIC 88206187 exhibits a fractional radius of the outer tertiary component (rB ≡ RB/aout) exceeding 0.1 (only the third such system known), and we consider its future evolution. Finally, we compare our photodynamical analysis results and the orbital parameters given in the Gaia DR3 NSS solutions, indicating decent agreement, but with the photodynamical results being more accurate.