bluuebunny/crossref_metadata_2025_split huggingface dataset:
Transcriptome analysis ofMedicago truncatulaAutoregulation of Nodulation mutants reveals that disruption of the SUNN pathway causes constitutive expression changes in a small group of genes, but the overall response to rhizobia resembles wild type, including induction ofTML1andTML2
Authors: Schnabel Elise L. , Chavan Suchitra A. , Gao Yueyao , Poehlman William L. , Feltus F. Alex , Frugoli Julia A. 
Published: January 2023

AbstractNodule number regulation in legumes is controlled by a feedback loop that integrates nutrient and rhizobia symbiont status signals to regulate nodule development. Signals from the roots are perceived by shoot receptors, including a CLV1-like receptor-like kinase known as SUNN in the annual medicMedicago truncatula. In the absence of functional SUNN, the autoregulation feedback loop is disrupted, resulting in hypernodulation. To elucidate early autoregulation mechanisms disrupted inSUNNmutants, we searched for genes with altered expression in the loss-of-functionsunn-4mutant and included therdn1-2autoregulation mutant for comparison. We identified constitutively altered expression of small groups of genes insunn-4roots, including higher levels of transcription factorNF-YA2, and insunn-4shoots. All genes with verified roles in nodulation that were induced in wild type roots during the establishment of nodules were also induced insunn-4, including, surprisingly, autoregulation genesTML2andTML1. Among all genes with a differential response to rhizobia in wild type roots, only an isoflavone-7-O-methyltransferase gene (Medtr7g014510) was found to be unresponsive insunn-4. In shoot tissues of wild type, eight rhizobia-responsive genes were identified, including a MYB family transcription factor gene (Medtr3111880) which remained at a baseline level insunn-4; three genes were found to be induced by rhizobia in shoots ofsunn-4but not wild type. We also cataloged the temporal induction profiles of many small secreted peptide (MtSSP) genes in nodulating root tissues, encompassing members of twenty-four peptide families, including the CLE and IRON MAN families. The discovery that expression ofTMLgenes in roots, a key factor in inhibiting nodulation in response to autoregulation signals, is also triggered insunn-4in the section of roots analyzed suggests that the mechanism of TML regulation inM. truncatulamay be more complex than published models.
Maturation of glutamatergic transmission onto dorsal raphe serotonergic neurons
Authors: Kisner Alexandre , Polter Abigail M. 
Published: January 2023

AbstractSerotonergic neurons in the dorsal raphe nucleus (DRN) play important roles early in postnatal development in the maturation and modulation of higher order emotional, sensory, and cognitive circuitry. This unique position makes these cells a substrate by which early experience can be wired into brain. In this study, we have investigated the maturation of synapses onto dorsal raphe serotonergic neurons in typically developing male and female mice using whole-cell patch-clamp recordings inex vivobrain slices. We show that while inhibition of these neurons is relatively stable across development, glutamatergic synapses greatly increase in strength between P6 and P21-23. In contrast to forebrain regions, where the components making up glutamatergic synapses are dynamic across early life, we find that the makeup of these synapses onto DRN serotonergic neurons is largely stable after P15. DRN excitatory synapses maintain a very high ratio of AMPA to NMDA receptors and a rectifying component of the AMPA response throughout the lifespan. Overall, these findings reveal that the development of serotonergic neurons is marked by a significant refinement of glutamatergic synapses during the first 3 postnatal weeks. This suggests this time as a sensitive period of heightened plasticity for integration of information from upstream brain areas and that genetic and environmental insults during this period could lead to alterations in serotonergic output, impacting both the development of forebrain circuits and lifelong neuromodulatory actions.
Competing differentiation gradients coordinate fruit morphogenesis
Authors: Gómez-Felipe A. , Marconi M. , Branchini E. , Wang B. , Bertrand-Rakusova H. , Stan T. , Burkiewicz J. , de Folter S. , Routier-Kierzkowska A-L. , Wabnik K. , Kierzkowski D. 
Published: January 2023

ABSTRACTMorphogenesis requires the coordination of cellular behaviors along developmental axes1. In plants, gradients of growth and differentiation are typically established along a single longitudinal primordium axis to control organ shaping2. Here we combine quantitative live-imaging at cellular resolution with genetics, chemical treatments, and modeling to understand the formation ofArabidopsis thalianafemale reproductive organ (gynoecium). We show that, contrary to other aerial organs, gynoecium shape is determined by two competing differentiation gradients positioned along two orthogonal axes. An early mediolateral gradient, dependent on meristematic activity in the medial domain, controls the valve morphogenesis while simultaneously restricting an auxin-dependent, longitudinal gradient to the style. This gradient competition serves to finetune the common developmental program governing organ morphogenesis to ensure the specialized function of the gynoecium3,4.
The nuclear to cytoplasmic ratio drives cellularization in the close animal relativeSphaeroforma arctica
Authors: Olivetta Marine , Dudin Omaya 
Published: January 2023

SummaryThe ratio of nuclear content to cytoplasmic volume (N/C ratio) is a key regulator driving maternal-to-zygotic transition in most animal embryos. Altering this ratio often impacts zygotic genome activation and deregulates the timing and outcome of embryogenesis [1–3]. Despite being ubiquitous across animals, little is known about when the N/C ratio evolved to control multicellular development. Such capacity either originated with the emergence of animal multicellularity or was co-opted from mechanisms present in unicellular organisms [4]. An effective strategy to tackle this question is to investigate close relatives of animals exhibiting life cycles with transient multicellular stages [5]. Among these are ichthyosporeans, a lineage of protists undergoing coenocytic development followed by cellularization and cell release [6–8]. During cellularization, a transient multicellular stage resembling animal epithelia is generated offering a unique opportunity to examine whether the N/C ratio regulates multicellular development. Here, we use time-lapse microscopy to characterize how the N/C ratio affects the life cycle of the best-studied ichthyosporean model,Sphaeroforma arctica. We uncover that the last stages of cellularization coincide with a significant increase in the N/C ratio. Increasing the N/C ratio by reducing the coenocytic volume accelerates cellularization while decreasing the N/C ratio by lowering the nuclear content halts it. Moreover, centrifugation and pharmacological inhibitor experiments suggest that the N/C ratio is locally sensed at the cortex and relies on phosphatase activity. Altogether, our results show that the N/C ratio drives cellularization inS. arctica, suggesting that its capacity to control multicellular development predates animal emergence.
C-terminal conformational changes in SCF-D3/MAX2 ubiquitin ligase are required for KAI2-mediated signaling
Authors: Tal Lior , Guercio Angelica , Varshney Kartikye , Young Aleczander , Gutjahr Caroline , Shabek Nitzan 
Published: January 2023

AbstractKarrikins (KARs) are bioactive molecules derived from burning vegetation. Plants perceive KARs through the α/β hydrolase KARRIKIN INSENSITIVE 2 (KAI2) that interacts with the F-box protein ubiquitin ligase MORE AXILLARY GROWTH 2 (MAX2). MAX2 also plays a role in the perception and signal activation by Strigolactone (SL), a phytohormone controlling various developmental processes in plants. SL also acts as a rhizosphere signal to activate arbuscular mycorrhiza fungi that can be exploited by parasitic plants.kai2knockouts exhibit distinct developmental defects and therefore KAI2 is hypothesized to perceive an unidentified endogenous ligand provisionally termed KAI2-Ligand (KL). Upon KAR/KL perception, the protein complex of KAI2-MAX2 targets SUPPRESSOR OF MAX2-1/2 (SMAX1)/SMXL2 for proteasomal degradation. Despite the identification of the key components KAI2, MAX2, and SMAX1 in KAR/KL signaling, their mode of interaction and regulation remains elusive. Recently, the regulatory function of the conformational switch of MAX2 C-terminal helix (CTH) in SL signaling has been demonstrated however its role in KAR/KL signaling remained unknown. Here we address the function of MAX2-CTH dynamics bothin vitroandin plantaand show that the central role of CTH is conserved between SL and KAR/KL signaling pathway.
Both behavior-manipulating and non-manipulating entomopathogenic fungi affect rhythmic gene expression in carpenter ant foragers upon infection
Authors: Das Biplabendu , Brachmann Andreas , de Bekker Charissa 
Published: January 2023

ABSTRACTBackgroundBehavioral plasticity in the nocturnal antCamponotus floridanusis associated with changes in daily rhythms of core clock and clock-controlled genes in the brain. Plasticity in clock-controlled output, although adaptive, has been hypothesized to be a target for parasites that change host behavior in a timely manner to complete their life cycle. This study aims to explore this hypothesis by characterizing how the transcriptomic rhythms of the ant host change upon infection by a behavior manipulating parasite. We compared and contrasted the daily gene expression profile of uninfectedC. floridanusant heads to ants infected by a manipulating fungal parasiteOphiocordyceps camponoti-floridaniand a non-manipulating fungusBeauveria bassiana, to test if changes to host clock and clock-controlled gene expression are specific to behavioral modifying diseases, or if such changes are a general hallmark of infectious diseases.ResultsThe repertoire of genes oscillating every 24h in the ant heads showed almost three-fold reduction duringO. camponoti-floridaniinfections, as compared to uninfected controls. Control-like nocturnal activity of 24h-rhythmic genes was maintained duringO. camponoti-floridaniinfections, but not inB. bassianainfected ant heads. Half of all genes that showed 24h rhythms in the heads and brains of uninfected ants displayed highly synchronized changes in their rhythmic expression during both diseases, but in a species-specific manner. Network analyses revealed that both fungal parasites affected the same links between behavioral plasticity and clock output, albeit in a different manner.ConclusionChanges to clock-controlled transcriptomic rhythms of hosts might be a general hallmark of infectious diseases. However, the infection-associated changes to clock-controlled rhythms of the host are species-specific, and likely depends on the life history strategies used by the parasite.
Evaluation of the impact of PEPFAR transition on retention in care in South Africa’s Western Cape
Authors: Chiliza Jessica , Brennan Alana T , Laing Richard , Feeley Frank Goodrich 
Published: January 2023

ABSTRACTBackgroundResearch on the impact of the PEPFAR transition in South Africa (SA) in 2012 found varying results in retention in care (RIC) of people living with HIV (PLWH).ObjectivesWe investigated the factors that impacted RIC during the U.S. President’ s Emergency Plan for AIDS Relief (PEPFAR) transition in Western Cape, South Africa in 2012.MethodsWe used aggregate data from 61 facilities supported by four non-governmental organizations from to 2007-2015. The main outcome was RIC at 12-months after antiretroviral therapy initiation for two time periods. We used quantile regression to estimate the effect of the PEPFAR pull-out and other predictors on RIC. The models were adjusted for various covariates.ResultsRegression models (50thquantile) for 12-month RIC showed a 4.6% (95%CI: -8.4, - 0.8%) decline in RIC post direct service. Facilities supported by Anova/Kheth’ impilo fared worst post PEFPAR; a decline in RIC of (−5.8%; 95% CI: -9.7, -1.8%), while that’ sit fared best (6.3% increase in RIC; 95% CI:2.5,10.1%). There was a decrease in RIC when comparing urban to rural areas (−5.8%; 95% CI: -10.1, -1.5%). City of Cape town combined with Western Cape Government Health facilities showed a substantial decrease (−9.1%; 95% CI: -12.3, -5.9%), while community health clinic (vs. primary health clinic) declined slightly (−4.4; 95% CI: -9.6, 0.9%) in RIC. We observed no RIC difference by facility size and a slight increase when two or more human resources transitioned from PEPFAR to the government.ConclusionsWhen PEPFAR funding decreased in 2012, there was a decrease in RIC. To ensure the continuity of HIV care when a major funder withdraws sufficient and stable transition resources, investment in organizations that understand the local context, joint planning, and coordination are required.
Biologic DMARD Access and Medication Cost-related Nonadherence in Rheumatology Patients in Canada: A Cross-sectional Survey
Authors: Holbrook Anne Marie , Ding Avrilynn , Troyan Sue , Costa Sandra , Matsos Mark , Law Michael R. , Gong Selena , Dixit Apurva , Foster Gary , Khalidi Nader 
Published: January 2023

AbstractBackgroundCost-related nonadherence to prescription medications affects many Canadians and is associated with negative self-perceptions of health. Biologic disease modifying anti-arthritic drugs (bDMARDs) are costly drugs recommended for certain patients with rheumatoid or psoriatic arthritis and ankylosing spondylitis. We investigated access and cost-related nonadherence (CRN) to bDMARDs compared to other therapies for such patients in Ontario.MethodsWe conducted a cross-sectional telephone survey of adult patients recruited from two academic rheumatology practices in Hamilton, Ontario, asking demographic and socioeconomic characteristics, drug plan coverage, medication cost-related cutbacks, opinions on the value of bDMARDs, and assistance with costs from health professionals. CRN was defined by patient self-report of not using or using less than prescribed amount of medication, due to cost.Results104 patients (mean age (SD) 61(12) years) participated, including 77 (74%) women, 57 (54.8%) taking bDMARDs, and 27 (25.9%) with household income <$40,000 annually. CRN was reported by 19 (18.3%) participants with no significant difference between those taking versus not taking bDMARDs (risk difference (95% CI): -0.10 (−0.25, 0.04); p=0.19). 37 (64.9%) of those taking bDMARDs reported that they would not take them if they had to pay the full cost. Overall, few patients reported that they would ask their doctor (17.3%) or pharmacist (15.4%) for help with reducing prescription costs.ConclusionCRN prevalence was relatively high amongst these rheumatology patients despite access to public and private funding mechanisms. Patients expressed a reluctance to ask their doctor or pharmacist for help in reducing their medication costs.
Prediction of the temperature–time history in ordinary bodies induced by surface heat flux utilizing the enhanced method of discretization in time and the finite difference method
Authors: Campo Antonio , Celentano Diego , Masip Yunesky 
Published: January 2023

Purpose
The purpose of this paper is to address unsteady heat conduction in two subsets of ordinary bodies. One subset consists of a large plane wall, a long cylinder and a sphere in one dimension. The other subset consists of a short cylinder and a large rectangular bar in two dimensions. The prevalent assumptions in the two subsets are: constant initial temperature, uniform surface heat flux and thermo-physical properties invariant with temperature. The engineering applications of the unsteady heat conduction deal with the determination of temperature–time histories in the two subsets using electric resistance heating, radiative heating and fire pool heating.

Design/methodology/approach
To this end, a novel numerical procedure named the enhanced method of discretization in time (EMDT) transforms the linear one-dimensional unsteady, heat conduction equations with non-homogeneous boundary conditions into equivalent nonlinear “quasi–steady” heat conduction equations having the time variable embedded as a time parameter. The equivalent nonlinear “quasi–steady” heat conduction equations are solved with a finite difference method.

Findings
Based on the numerical computations, it is demonstrated that the approximate temperature–time histories in the simple subset of ordinary bodies (large plane wall, long cylinder and sphere) exhibit a perfect matching over the entire time domain 0 < t < ∞ when compared against the rigorous exact temperature–time histories expressed by classical infinite series. Furthermore, using the method of superposition of solutions in the convoluted subset (short cylinder and large rectangular crossbar), the same level of agreement in the approximate temperature–time histories in the simple subset of ordinary bodies is evident.

Originality/value
The performance of the proposed EMDT coupled with a finite difference method is exhaustively assessed in the solution of the unsteady, one-dimensional heat conduction equations with prescribed surface heat flux for: a subset of one-dimensional bodies (plane wall, long cylinder and spheres) and a subset of two-dimensional bodies (short cylinder and large rectangular bar).
Exploring mobile banking service quality dimensions in Pakistan: a text mining approach
Authors: Hussain Atif , Hannan Abdul , Shafiq Muhammad 
Published: January 2023

PurposeCustomer reviews of mobile banking (m-banking) apps contain the most direct and first-hand accounts of customer experiences with m-banking. However, surprisingly little effort has been made to understand m-banking service quality using these reviews. Therefore, this study aims to discover m-banking service quality dimensions from customers' reviews of the m-banking apps through a text mining approach.Design/methodology/approachReviews of m-banking apps of 24 banks operating in Pakistan were scraped from Google Play Store. Latent Dirichlet allocation (LDA) method was applied to discover the dimensions of m-banking service quality from 24,529 positive and 29,569 negative useable reviews.FindingsDifferent dimensions of m-banking service quality are discussed in positive and negative reviews. Positive reviews focus on security, convenience, ease of use, continuous improvement, usefulness and app attributes, whereas negative reviews discuss system availability, responsiveness, faulty updates, login problems and reliability.Research limitations/implicationsThe results are based only on customer reviews in one country and generalization may not be possible. Moreover, due to the unavailability of demographic information about reviewers, the effect of demographic characteristics on users' perceptions of m-banking quality could not be determined.Practical implicationsThe study provides managers with useful insights to improve the service experience of m-banking customers. The study also demonstrates how managers can employ text analytical techniques to assess and improve the quality of m-banking services.Originality/valueIn addition to enriching the understanding of m-banking quality based on direct and first-hand user experiences, the current study also provides initial evidence for the two-factor structure of m-banking service quality.
Sponsorship and social justice: brand positioning on diversity and inclusion in sport marketing during the 2020 UEFA European football championship
Authors: Beek Remco , Van Hoecke Jo , Derom Inge 
Published: January 2023

PurposeContextual changes in communications, social activism and perceptions of commercialization have changed the dynamics in sponsorship. This paper investigated the patterns in sponsorship and social justice within the context of a major sports event.Design/methodology/approachThe European Football Championship serves as an impactful platform for sponsors due to its global reach. The sponsorship activations of the twelve official sponsors were investigated by analysing sponsorship expressions on the LED boarding during every match of the tournament. Furthermore, additional data on sponsorship characteristics and brand positioning was collected for every sponsor to define relevant factors to understand the differences in sponsorship communications.FindingsDuring UEFA EURO 2020, five official sponsors changed their sponsorship activations. Adjustments were made in sponsorship expressions to position the brand on diversity and inclusion. The analyses of over 90,000 press photos and 51 official match videos clarified the dynamics of brand positioning, sponsorship characteristics in perspective of globalization patterns and different sponsorship approaches in different geographical, social and political contexts.Practical implicationsDecision makers in the global sports industry are challenged in their brand management and sponsorship approaches for the social good. This study supports to understand the sponsorship approaches and factors affecting these different strategies.Originality/valueDespite the growing attention to social justice issues in sports, there is a need to understand inclusive marketing strategies in sponsorship relationships. Using data triangulation, the findings enabled to clarify differences in sponsor approaches to social justice and illustrate the complex dynamics of brand positioning on diversity and inclusion in the sponsorship ecosystem.
Pivotal stimulants to halal certified firms: a case study of Taiwan
Authors: Amalia Fatya Alty , Lestari Yuliani Dwi , Wang Kung-Jeng , Saidah Faridatus , Putri Aghnia Nadhira Aliya 
Published: January 2023

Purpose
This study aims to investigate the drivers of halal-certified firms in Taiwan, a Muslim minority country, to provide halal products or services.

Design/methodology/approach
This study used a quantitative method by spreading offline and online surveys toward halal-certified firms in Taiwan. Data were obtained from 41 responses and were analyzed using partial least square structural equation modeling.

Findings
This study discovers that firms in Taiwan practice halal business as they possess halal perceived value and innovation orientation. In addition, the interplays of the antecedents, the internal and external drivers, also play an excellent role in shaping the firms’ practices on halal business.

Research limitations/implications
Despite the implications, this study was built under several limitations that are potentially addressed by future research. The limitations lay in the low response rate, indistinctive analysis based on the firms’ market orientations (domestic or international) and the exclusion of other relevant variables to the conceptual model.

Practical implications
Practically, this study clarifies that firms in a Muslim minority country can be encouraged to engage in halal business in several ways. Those with a good understanding of halal usefulness are oriented to innovation, exposed to external pressures about halal business and equipped with specific internal settings that can translate the halal business phenomenon as an opportunity, not a threat.

Originality/value
To the best of the authors’ knowledge, this study is the first to unveil the drivers of halal business practices of firms in a Muslim minority country.