bluuebunny/crossref_metadata_2025_split huggingface dataset:
Research on primary frequency regulation strategies for ancillary wind power inertia based on the rotor kinetic energy
Authors: Sun Li , Yang Peihong , Jiang Hui , Xu Yanfang , Kang Lan , Lu Xiuyan 
Published: January 2023

The additional frequency control of wind turbines is an effective method to solve the problem of low inertia in power systems with high proportions of new energy. The primary frequency regulation of auxiliary wind power inertia systems based on rotor kinetic energy can not only make the wind turbine run at the maximum power point but also has the lowest cost and better economy of the auxiliary frequency regulation module. The wind power inertia output control scheme based on rotor kinetic energy control is constructed by considering the frequency response characteristics of synchronous generator sets and loads. The calculation model of the minimum inertia demand of the power system is established using the rate of change of frequency and the maximum frequency offset as constraints. Combined with the real-time operating conditions of the wind turbine, the speed regulation limit of the wind turbine rotor kinetic energy control is obtained to avoid wind turbine off-grid due to excessive frequency regulation. To prevent frequency secondary drop of the system during the speed recovery process, the steady speed recovery of the wind turbine is controlled by setting the rate of speed change. The feasibility of the strategy for the regulation of the auxiliary primary frequency proposed in this study was verified in an example based on a two-region, four-machine system. When a disturbance sets the sudden load power to 150 MW, under the kinetic energy control of the wind turbine rotor, the system frequency change rate and the maximum frequency offset are increased; in particular, the maximum frequency offset is reduced by 0.348 Hz, which further illustrates the flexibility and plasticity of the rotor kinetic energy control of the wind turbines. The results of this study provide a theoretical basis for adding additional frequency control to existing wind turbines.
Prediction of flash flood susceptibility using integrating analytic hierarchy process (AHP) and frequency ratio (FR) algorithms
Authors: Majeed Muhammad , Lu Linlin , Anwar Muhammad Mushahid , Tariq Aqil , Qin Shujing , El-Hefnawy Mohamed E. , El-Sharnouby Mohamed , Li Qingting , Alasmari Abdulrahman 
Published: January 2023

The landscape of Pakistan is vulnerable to flood and periodically affected by floods of different magnitudes. The aim of this study was aimed to assess the flash flood susceptibility of district Jhelum, Punjab, Pakistan using geospatial model and Frequency Ratio and Analytical Hierarchy Process. Also, the study considered eight most influential flood-causing parameters are Digital Elevation Model, slop, distance from the river, drainage density, Land use/Land cover, geology, soil resistivity (soil consisting of different rocks and soil formation) and rainfall deviation. The rainfall data was collected from weather stations in the vicinity of the study area. Estimated weight was allotted to each flood-inducing factors with the help of AHP and FR. Through the use of the overlay analysis, each of the factors were brought together, and the value of drainage density was awarded the maximum possible score. According to the study several areas of the region based on the parameters have been classified in flood zones viz, very high risk, high risk, moderate risk, low risk, and very low risk. In the light of the results obtained, 4% of the study area that accounts for 86.25 km2 is at high risk of flood. The areas like Bagham, Sohawa, Domeli, Turkai, Jogi Tillas, Chang Wala, Dandot Khewra were located at the very high elevation. Whereas Potha, Samothi, Chaklana, Bagrian, Tilla Jogian, Nandna, Rawal high-risk zones and have been damaged badly in the flood history of the area. This study is the first of its kind conducted on the Jhelum District and provides guidelines for disaster management authorities and response agencies, infrastructure planners, watershed management, and climatologists.
Whole genome sequencing of Clarireedia aff. paspali reveals potential pathogenesis factors in Clarireedia species, causal agents of dollar spot in turfgrass
Authors: Bahri Bochra Amina , Parvathaneni Rajiv Krishna , Spratling Willis Turner , Saxena Harshita , Sapkota Suraj , Raymer Paul L. , Martinez-Espinoza Alfredo D. 
Published: January 2023

Dollar spot is one of the most damaging diseases in turfgrass, reducing its quality and playability. Two species, Clarireedia monteithiana and C. jacksonii (formerly Sclerotinia homoeocarpa) have been reported so far in the United States To study the Clarireedia genome, two isolates H2 and H3, sampled from seashore paspalum in Hawaii in 2019 were sequenced via Illumina paired-end sequencing by synthesis technology and PacBio SMRT sequencing. Both isolates were identified as C. aff. paspali, a novel species in the United States Using short and long reads, C. aff. paspali H3 contained 193 contigs with 48.6 Mbp and presented the most completed assembly and annotation among Clarireedia species. Out of the 13,428 protein models from AUGUSTUS, 349 cytoplasmic effectors and 13 apoplastic effectors were identified by EffectorP. To further decipher Clarireedia pathogenicity, C. aff. paspali genomes (H2 and H3), as well as available C. jacksonii (LWC-10 and HRI11), C. monteithiana (DRR09 and RB-19) genomes were screened for fifty-four pathogenesis determinants, previously identified in S. sclerotiorum. Seventeen orthologs of pathogenicity genes have been identified in Clarireedia species involved in oxalic acid production (pac1, nox1), mitogen-activated protein kinase cascade (pka1, smk3, ste12), appressorium formation (caf1, pks13, ams2, rgb1, rhs1) and glycolytic pathway (gpd). Within these genes, 366 species-specific SNPs were recorded between Clarireedia species; twenty-eight were non-synonymous and non-conservative. The predicted protein structure of six of these genes showed superimposition of the models among Clarireedia spp. The genomic variations revealed here could potentially lead to differences in pathogenesis and other physiological functions among Clarireedia species.
Ruxolitinib does not completely abrogate the functional capabilities of TLR4/9 ligand-activated NK cells
Authors: Mestre-Durán Carmen , Martín-Cortázar Carla , García-Solís Blanca , Pernas Alicia , Pertíñez Lidia , Galán Víctor , Sisinni Luisa , Clares-Villa Laura , Navarro-Zapata Alfonso , Al-Akioui Karima , Escudero Adela , Ferreras Cristina , Pérez-Martínez Antonio 
Published: January 2023

IntroductionNatural killer (NK) cells are lymphocytes from the innate immune system part of the first defense barrier against infected and transformed cells, representing 5%-15% of peripheral blood lymphocytes. The cytotoxic capacity of NK cells is controlled by a balance between inhibitory and activating NK receptors expressed on their surface, which recognize and interact with the ligands on stressed cells. The cytokines involved in NK cell activation, proliferation, survival, and cytotoxicity are signaled mainly through the Janus kinase and signal transducer and activator of transcription proteins (JAK/STAT) pathway. NK cells are also activated in response to pathogens through Toll-like receptors (TLRs) expressed on their surface. Ruxolitinib is a specific JAK1/2 inhibitor approved for treating myelofibrosis and for steroid-refractory acute and chronic graft-versus-host disease (SR-GvHD).MethodsPurified NK cells from healthy donors were stimulated with two TOLL-like receptor ligands, LPS and CpG, in the presence of different concentrations of Ruxolitinib.ResultsThis study showed the effects of ruxolitinib on TLR4 and TLR9 ligand-activated NK cells from healthy donors. Ruxolitinib did not completely inhibit STAT3 phosphorylation and had a moderate effect on NK cell cytokine activation via the TLR pathway. Only the highest doses of ruxolitinib led to a decrease in the pro-inflammatory cytokines tumor necrosis factor α, interferon-γ, interleukin-6, and interleukin-1β. The cytotoxic capacity of stimulated NK cells versus K562, SEM, and MV-4-11 cell lines was reduced by increasing doses of ruxolitinib, but it was not completely abolished and we observed no major changes in degranulation capacity. Phenotypic changes were observed in activated NK cells in the presence of ruxolitinib. In a small cohort of pediatric patients treated with ruxolitinib for SR-GvHD, we observed no decrease in NK cell counts; however, further prospective studies with larger cohorts are necessary to confirm this finding.DiscussionIn summary, our results showed that the functional capabilities and phenotype of NK cells activated through TLR4/9 agonists were not completely abolished by the inhibition of the JAK-STAT pathway by ruxolitinib.
Paradoxical phenomena of bullous pemphigoid induced and treated by identical biologics
Authors: Zhang Jie , Wang Si-Hang , Zuo Ya-Gang 
Published: January 2023

ObjectiveThis study aimed to investigate the clinical features of biologics-induced bullous pemphigoid (BP) and the therapeutic effects of those agents for BP, exploring the underlying pathophysiological mechanisms.MethodsWe searched PubMed, Web of Science, and Elsevier for studies involving pemphigoid patients treated with or induced by identical biologics published in English from January 2009 to April 2022.ResultsSeventeen cases of drug-induced BP associated with anti-tumor necrosis factor (aTNF)-α therapies, one with interleukin (IL)-17 inhibitors, and seven with IL-12/IL-23 or IL-23 inhibitors were enrolled. Time to cutaneous toxicity varied among different types of agents, and the characteristics of clinical examinations were similar to idiopathic BP. Discontinuation of the culprit drugs and initiation of topical or systemic corticosteroids were adequate in most cases. Several monoclonal antibodies above have also been reported for the treatment of refractory or recurrent BP, especially concurrent with psoriasis.ConclusionBiologics for immune-related diseases, including TNF-α, IL-17, and IL-12/IL-23 or IL-23 inhibitors, can both induce and treat BP, which might be associated with a helper T cells Th1/Th2 imbalance, complicated inflammatory networks, and a specific individual microenvironment, suggestive of a new perspective on the therapeutic algorithms of BP. There have been numerous reports about biologics inducing or treating BP. We have taken note of this phenomenon and focused on biologics with both pathogenetic and therapeutic effects on BP. Our review summarized the clinical characteristics of associated cases, trying to figure out the underlying mechanisms of this paradoxical phenomenon and to provide an integrated perspective and new therapeutic alternatives for BP.
The making of multivalent gamma delta TCR anti-CD3 bispecific T cell engagers
Authors: van Diest Eline , Nicolasen Mara J. T. , Kramer Lovro , Zheng Jiali , Hernández-López Patricia , Beringer Dennis X. , Kuball Jürgen 
Published: January 2023

IntroductionWe have recently developed a novel T cell engager concept by utilizing γ9δ2TCR as tumor targeting domain, named gamma delta TCR anti-CD3 bispecific molecule (GAB), targeting the phosphoantigen-dependent orchestration of BTN2A1 and BTN3A1 at the surface of cancer cells. GABs are made by the fusion of the ectodomains of a γδTCR to an anti-CD3 single chain variable fragment (scFv) (γδECTO-αCD3), here we explore alternative designs with the aim to enhance GAB effectivity.MethodsThe first alternative design was made by linking the variable domains of the γ and δ chain to an anti-CD3 scFv (γδVAR-αCD3). The second alternative design was multimerizing γδVAR-αCD3 proteins to increase the tumor binding valency. Both designs were expressed and purified and the potency to target tumor cells by T cells of the alternative designs was compared to γδECTO-αCD3, in T cell activation and cytotoxicity assays.Results and discussionThe γδVAR-αCD3 proteins were poorly expressed, and while the addition of stabilizing mutations based on finding for αβ single chain formats increased expression, generation of meaningful amounts of γδVAR-αCD3 protein was not possible. As an alternative strategy, we explored the natural properties of the original GAB design (γδECTO-αCD3), and observed the spontaneous formation of γδECTO-αCD3-monomers and -dimers during expression. We successfully enhanced the fraction of γδECTO-αCD3-dimers by shortening the linker length between the heavy and light chain in the anti-CD3 scFv, though this also decreased protein yield by 50%. Finally, we formally demonstrated with purified γδECTO-αCD3-dimers and -monomers, that γδECTO-αCD3-dimers are superior in function when compared to similar concentrations of monomers, and do not induce T cell activation without simultaneous tumor engagement. In conclusion, a γδECTO-αCD3-dimer based GAB design has great potential, though protein production needs to be further optimized before preclinical and clinical testing.
A high interferon gamma signature of CD8+ T cells predicts response to neoadjuvant immunotherapy plus chemotherapy in gastric cancer
Authors: Li Sen , Li Ke , Tian Fei , Li Hongle , Xia Qingxin , Li Tiepeng , Dong Bing , Li Danyang , Yu Juan , Zhang Junli , Wang Li , Zhang Chengjuan , Xu Shuning , Zhao Yuzhou , Liu Ying 
Published: January 2023

BackgroundWhile the tumor microenvironment (TME) affects immune checkpoint blockade (ICB) efficacy, ICB also reshapes the characteristics of TME. Thus far, studies have focused on the TME evolution during neoadjuvant or adjuvant ICB therapy in gastric cancer (GC). However, the interaction between TME characteristics and neoadjuvant immunotherapy plus chemotherapy remains to be elucidated.MethodsWe performed single-cell RNA sequencing on ten GC specimens pre- and post-neoadjuvant camrelizumab plus mFOLFOX6 to determine the impact of the TME on the efficacy of the combination therapy and the remodeling of TME by the therapy.ResultsA high baseline interferon gamma (IFN-γ) signature in CD8+ T cells predicts better responses to the combination therapy. We also observed that the IFN-γ signature significantly decreased in multiple cell types, and the exhausted signature of CD8+ T cells was significantly suppressed during the neoadjuvant therapy.ConclusionsOur data reveal interactions between the TME and neoadjuvant immunotherapy plus chemotherapy in GC. Importantly, it also highlights the signature of CD8+ T cells in predicting response to the combination therapy in GC.
Effects of LED spectrum on circadian rhythmic expression of clock genes and Aanat2 in the brain of juvenile European seabass (Dicentrarchus labrax)
Authors: Ma He , Liu Songtao , Wei Pingping , Fei Fan , Ren Jilong , Dai Mingyun , Ma Zhen , Liu Ying 
Published: January 2023

The circadian rhythm is a physiological phenomenon that occurs in various organisms with a cycle of about 24 hours. Light is one of the important environmental factors affecting biological rhythm. To clarify whether a shift in light spectrum can influence the circadian expression in fish brain, a total of 175 European seabasses [body weight: 32.5 ± 0.71) g; body length: (13.78 ± 0.35) cm] were exposed to white light (WL), red light (RL), yellow light (YL), green light (GL) or blue light (BL). After 50 days of exposure, circadian expressions of four core clock genes (Clock, Bmal1, Per2, Cry1) and Aanat2 gene in brain were examined. The results showed that the temporal expression patterns of positive clock gens (Clock and Bmal1) showed increases during the scotophase and decreases during the photophase, with peaks near the middle of the darkness. Clock gene expression showed a stable circadian rhythm (R2 = 0.578-0.824, P=0.000- 0.027) in all light groups while Bmal1 showed circadian rhythm in WL, GL and RL, not in BL and YL. Daily expression patterns of the negative clock genes oscillated in the opposite phase from the positive clock genes, showing increasing mRNA levels during the light, decreases during the dark, and peaks near the shift from night to day, except Per2 in RL and Cry1 in BL. Compared with WL, the acrophases of Clock and Bmal1 were delayed under all light treatments (BL: + 3.7h, +6.73h; RL: +2.4h,+1.35h; YL: + 4.94h, 2.00h; GL: +0.05, +0.16h). Cry1 showed advanced acrophase under all light treatments (BL: -10.74 h, GL: -3.81 h, RL: -3.93 h, YL: -7.56 h) but Per2 showed delayed acrophase in all light treatments (GL: +0.86 h, RL: +10.35 h, YL: +9.62 h), except in BL (-0.43 h). The acrophase of Aanat2 was advanced by all monochromatic light, the Aanat2 level was significantly increased in RL compared with other light treatment. Therefore, the results indicate that RL may regulate the expression of Aanat2 gene by affecting the expression of clock gene in fish brain. Spectrum can affect the biological clock system of fish, and unreasonable spectrum may disturb the rhythm of gene expression of biological clock of fish. Under the irradiation of light spectrum, some clock genes still maintain obvious circadian oscillation, while the rhythm of some clock genes is not obvious and may be destroyed. Our findings suggest a primary role of light spectrum information to the fish brain circadian system.
Caloric restriction delays age-related muscle atrophy by inhibiting 11β−HSD1 to promote the differentiation of muscle stem cells
Authors: Lv Shan , Shen Qianjin , Li Hengzhen , Chen Qun , Xie Wenqing , Li Yusheng , Wang Xiaodong , Ding Guoxian 
Published: January 2023

IntroductionCalorie restriction (CR) is an important direction for the delay of sarcopenia in elderly individuals. However, the specific mechanisms of CR against aging are still unclear.MethodsIn this study, we used a CR model of elderly mice with muscle-specific 11β-hydroxysteroid dehydrogenase 1 (11β-HSD1) knockout mice and 11β-HSD1 overexpression mice to confirm that CR can delay muscle aging by inhibiting 11β-HSD1 which can transform inactive GC(cortisone) into active GC(cortisol). The ability of self-proliferation and differentiation into muscle fibers of these mouse muscle stem cells (MuSCs) was observed in vitro. Additionally, the mitochondrial function and mitochondrial ATP production capacity of MuSCs were measured by mitochondrial oxygen consumption.ResultsIt was found that the 11β-HSD1 expression level was increased in age-related muscle atrophy. Overexpression of 11β-HSD1 led to muscle atrophy in young mice, and 11β-HSD1 knockout rescued age-related muscle atrophy. Moreover, CR in aged mice reduced the local effective concentration of glucocorticoid (GC) through 11β-HSD1, thereby promoting the mitochondrial function and differentiation ability of MuSCs.ConclusionsTogether, our findings highlight promising sarcopenia protection with 40% CR in older ages. Furthermore, we speculated that targeting an 11β-HSD1-dependent metabolic pathway may represent a novel strategy for developing therapeutics against age-related muscle atrophy.
The association of periodontal diseases and Sjogren’s syndrome: A systematic review and meta-analysis
Authors: Yang Bo , Pang Xuefei , Guan Jiazhong , Liu Xu , Li Xiting , Wang Yan , Chen Zhuofan , Cheng Bin 
Published: January 2023

BackgroundThe relationship between periodontal diseases and Sjogren’s syndrome were found inconsistent in current studies. Our objective is to clarify the relationship between periodontal diseases and Sjogren’s syndrome.MethodsA systematic review was performed and reported according to Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA). Electronic databases (EMBASE, PubMed, Web of Science, and Cochrane Library, from inceptions until 24 November 2021) were searched. The Newcastle-Ottawa Scale (NOS) and Agency for Healthcare Research and Quality (AHRQ) were applied to evaluate the quality of studies. Quality assessment of the certainty of evidence was performed based on the Grading of Recommendations Assessment, Development, and Evaluation (GRADE) guidelines. When the output is the ratio, Odds ratio (OR) of periodontal diseases with Sjogren’s syndrome were calculated. When the output is the mean, weighted mean difference (WMD) of periodontal diseases with Sjogren’s syndrome was calculated. We conducted meta-analysis and estimated the pool sensitivity. Begg’s test was used to test the possibility of publication bias. We also carried out meta-regression to clarify the source of heterogeneity (I2 &gt; 50%). Finally, we performed a trial sequential analysis (TSA) to identify the false positive or false negative outcomes that might occur during repeated updates.Results21 studies were included in this systematic review, with a total of 11435 subjects. Meta-analysis of 5 studies showed that there is a positive correlation between periodontitis and Sjogren’s syndrome (OR = 2.12, 95% CI = 1.43–3.17; 5 studies, 6927 participants; low certainty of evidence). Meta-analysis of 16 studies showed that the periodontal condition of patients with Sjogren’s syndrome was worse compared with the control group, and the scores of clinical periodontal parameters were relatively high.ConclusionSjogren’s syndrome patients seem to be more likely to be diagnosed with periodontal diseases. However, our results should be interpreted with caution considering the high heterogeneity.Systematic review registration[https://www.crd.york.ac.uk/prospero/], identifier [CRD42021261322].
Characterization, antibacterial, antioxidant, antidiabetic, and anti-inflammatory activities of green synthesized silver nanoparticles using Phragmanthera austroarabica A. G. Mill and J. A. Nyberg extract
Authors: Khodeer Dina M. , Nasr Ali M. , Swidan Shady A. , Shabayek Sarah , Khinkar Roaa M. , Aldurdunji Mohammed M. , Ramadan Maryam A. , Badr Jihan M. 
Published: January 2023

IntroductionDiabetes mellitus is a chronic metabolic disorder that exhibited great expansion all over the world. It is becoming an epidemic disease adding a major burden to the health care system, particularly in developing countries.MethodsThe plant under investigation in the current studyPhragmanthera austroarabicaA. G. Mill and J. A. Nyberg is traditionally used in Saudi Arabia for the treatment of diabetes mellitus. The methanolic extract (200 mg/kg) of the plant and pure gallic acid (40 mg/kg), a major metabolite of the plant, as well as their silver nanoparticle formulae (AgNPs) were evaluated for their antidiabetic activity.Results and DiscussionThe results showed a decrease in body fat, obesity, an improvement in lipid profiles, normalization of hyperglycemia, insulin resistance, and hyperinsulinemia, and an improvement in liver tissue structure and function. However, the results obtained from AgNPs for both extract and the pure gallic acid were better in most measured parameters. Additionally, the activity of both the crude extract of the plant and its AgNPs were evaluated against a number of gram-positive, gram-negative bacteria and fungi. Although the activity of the crude extract ranged from moderate to weak or even non-active, the AgNPs of the plant extract clearly enhanced the antimicrobial activity. AgNPs of the extract demonstrated remarkable activity, especially against the Gram-negative pathogensProteus vulgaris(MIC 2.5 μg/ml) andPseudomonas aeruginosa(MIC 5 μg/ml). Furthermore, a promising antimicrobial activity was shown against the Gram-positive pathogenStreptococcus mutants(MIC 1.25 μg/ml).