bluuebunny/crossref_metadata_2025_split huggingface dataset:
Penerapan Model Praktitikum Terintegrasi Problem Based Learning (PTPBL) Untuk Meningkatkan Kualitas Pelaksanaan Praktikum Pemisahan Analitik
Authors: Mutiah Mutiah , Andayani Yayuk , Anwar Yunita Arian Sani , Idrus Syarifa Wahidah Al , Junaidi Eka 
Published: December 2022

Penelitian ini tentang penerapan model praktikum terintegrasi problem based learning (PTPBL) pada mata kuliah pemisahan analitik dengan mengangkat suatu permasalahan nyata yang harus dipecahkan melalui praktikum. Tujuan dari penelitian ini adalah terlaksananya model praktikum terintegrasi problem based learning (PTPBL) untuk meningkatkan kualitas pelaksanaan praktikum pemisahan analitik. Untuk mencapai hal tersebut maka dilakukan penelitian tindakan kelas (action research) selama 3 siklus. Setiap siklus terdiri dari perencanaan, tindakan dan refleksi. Tindakan diawali dengan memberikan masalah, selanjutnya diselesaikan secara berkelompok melalui identifikasi masalah, perkuliahan, pencarian literatur, seminar, tutorial, demonstrasi, dan diakhiri dengan praktikum. Hasil penelitian menunjukkan adanya peningkatan pada indikator kualitas pelaksanaan PTPBL yaitu meningkatnya aspek kognitif, psikototorik dan sikap ilmiah mahasiswa. Respon mahasiswa terhadap pelaksanaan PTPBL sangat bagus dan menyatakan bahwa PTPBL menarik, tidak memberatkan, dan mahasiswa menjadi lebih paham akan konsep yang sedang dipelajari dan aplikasinya serta menimbulkan keinginan untuk mengembangkan ide terkait. Kesimpulan peenelitian in adalah model PTPBL dapat meningkatkan aktivitas siswa pada aspek sikap, ketrampilan dan pemahaman.
Mini-review on Inhibitors of Human Tyrosinase
Authors: Srivastava Jyoti , Mal Joyabrata , Verma Manju , Sinha Rupika 
Published: December 2022

AbstractMelanin is a major pigment of human skin that protects the skin from harmful ultraviolet radiation, DNA damage and oxidative stress. However, the excess accumulation of melanin may lead to various hyperpigmentation-related diseases. Tyrosinase is a copper containing enzyme that regulates the rate-limiting step of melanin synthesis. So, inhibiting tyrosinase is the crucial target for researchers for the treatment of hyperpigmentation. Unfortunately, almost all the literature is based on mushroom tyrosinase (mTYR) for their application on humans as pure human tyrosinase (hTYR) is difficult to isolate. Since presently used tyrosinase inhibitors are developed using mushroom tyrosinase, they are insufficient to match the affinity, selectivity and efficacy required to target the human tyrosinase. Therefore, there is an urgent need for identifying a selective tyrosinase inhibitor that matches the selectivity and safety standards of human tyrosinase. This mini-review is focused on the tyrosinase inhibitors developed and evaluated using human tyrosinase.
Phylogenetic Characterization of Nitrifying Bacteria Isolated from East Kolkata Wetland
Authors: Saha Mousumi , Sarkar Agniswar , Bandyopadhyay Bidyut 
Published: December 2022

AbstractEast Kolkata Wetland (EKW) is an “International Ramsar Site”, famous for broad biodiversity and insightful use of sewage for aquaculture. Native nitrifying bacteria of EKW play a significant role in maintaining water quality and controlling environmental pollution by converting ammonia into nitrate in wastewater. Therefore, the characterization of nitrifying bacteria is important in EKW. Thus, the main focus of this research was to identify and characterize the nitrifying bacteria, investigating their phylogeny and diversity in EKW. 16S rRNA and functional genes analysis may help in the proper evaluation of composition and distribution of nitrifying bacteria in some water bodies in EKW, which has not yet been explored. Molecular and phylogenetic characterization was targeted and achieved through 16S rRNA and functional gene analysis, followed by computational estimation. Resulted sequences were analysed to gain insight into the knowledge for global and local taxonomic orientation. Hence, a model can be created for characterizing the dynamics of nitrifying bacteria in wastewater treatment and sustainable aquaculture in different water bodies of EKW.
Graphical Abstract
A Review on Current Strategies for Biofilm Control in Food Industry
Authors: Verma Nidhi , Agarwal Vishnu 
Published: December 2022

AbstractBiofilms are still a serious threat to the world. Biofilms are formed due to the natural tendency of microorganisms according to environmental factors. And they are solicitude in many fields counting food, medical and environmental. Biofilms are hard to exterminate due to their resistant phenotype. Since biofilms is a surface episode it develops on the different surfaces in food industry which can be very severe for the consumers, because it can cause serious illness to the consumers as well as monetary loss. In the current scenario to prevent biofilm formation the basic protocols that are used are cleaning and disinfection which cannot remove biofilms properly. Consequently, the new strategies are developing along with improving conventional control methods. Use of enzymes, biosurfactants, electrostatic interactions, essential oils to prevent biofilm formation.This review intent on the present strategies that are in use or is developing for controlling biofilms. Which can offer statistics about major concerns in food industries.
In Vitro Anticancer Screening of Methanolic Extract of Stachytarpheta Mutabilis
Authors: Londonkar Ramesh , Kesralikar Maithilee 
Published: December 2022

AbstractIn therapeutic plants, phytochemicals are found in abundance. A family of verbena known as vervain (verbenaceae) consists of over 100 genera and nearly 2600 species. Among the Verbenaceae family is Stachytarpheta, a green perennial herb. The species is being examined in this study for its phytochemical, antioxidant, and anti-inflammatory properties. Stachytarpheta mutabilis methanol extracts were studied for their phytochemical screening, antimicrobial, anti-inflammatory, and anticancerous effects in the current study. Phytochemical study revealed the presence of alkaloids, flavonoids, glycosides, steroids, tannins, carbohydrates, and saponins, among other phytoconstituents. One fungus, Aspergillus niger, Staphylococcus aureus, and Bacillus subtilis, as well as four-gram (+) pathogens, including Staphylococcus aureus and Bacillus subtilis, and two gram (-) pathogens, including Escherichia coli, Pseudomonas aeruginosa, Salmonella typhi, and Klebsiella pneumoniae, were disc diffusion tested for antibacterial activity. The nitric oxide scavenging assay was used to test for anti-inflammatory efficacy in vitro. DPPH, ABTS, and total antioxidant tests were used to evaluate antioxidant activity. Analyses such as TLC, GC-MS, and were performed as per common procedure. A nitric oxide scavenging experiment was utilised to determine the anti-inflammatory properties of the drug in vitro. Tests for antioxidant activity were conducted using DPPH (2,2-diphenyl-1-picrylhydrazyl), ABTS (2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)), and total antioxidant tests. MTT (3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide) analysis has been used to do anticancer screening. Extracts from Stachytarpheta mutabilis were found to include flavonoids, tannins and phenolic compounds as well as terpenoids and steroids in their phytochemical analysis. Squalene, octadecanal, and butan-1-one were found in the GC-MS study as were thirty other bioactive chemicals such as 4-isopropylcinnamic acid and octadecanoic acid. The methanolic fractions of plant extracts were shown to be effective against all bacteria. Anti-inflammatory and antioxidant experiments performed in the lab revealed the compounds’ potent inhibition activity. Analysis of the GC-MS data revealed the existence of thirty distinct components. MTT assay on the extract revealed anticancer activity. The data shows that the methanolic extract has a significant properties. Stachytarpheta mutabilis has therapeutic value and other pharmaceutical qualities may be assessed as a result of these results, which support the traditional usage of folk medicine as well.
Genome Scale Pathway-Pathway Co-functional Synergistic Network (PcFSN) in Oryza Sativa
Authors: Mal Chittabrata , Banerjee Ayushman Kumar , Mal Joyabrata 
Published: December 2022

AbstractCohesive network modelling and systems biology have emerged as extremely potent tools which helps understanding the combinatorial effects of biomolecules. Synergistic modulation among biomolecules (e.g., enzymes, transcription factors, microRNAs, drugs, etc.) are significant in finding out complex regulatory mechanisms in biological networks and pathways. In some cases, although combinatorial interactions among some biomolecules in specific biological networks is available, our knowledge in that particular domain is very limited with context to a genomic scale. Here we explore the pathway-pathway network to identify and understand the network architecture of metabolic pathway mediated regulations at genomic and co-functional levels, in rice. Using network transformation methods, a genome scale pathway-pathway co-functional synergistic network (PcFSN) was constructed. Finally, the PcFSN modules are extracted. This in turn helps to identify the miRNAs and genes associated with the pathways, especially linked to the central metabolic network in rice.
Mulhouse Workers' Town (1853−1897): Industrialism, rationalism and settlements
Authors: Filippov V. D. 
Published: December 2022

The paper describes the history of the of Mulhouse, a working-class town in France found in 1853. Shown is the connection of its foundation with local social conditions and industrialism of Henri de Saint-Simon.The conditions of the workers' town emergence and the Soviet workers' settlements in the 1930s are compared to identify the reasons for the similarity in the architectural design. The development history of the Workers' Town is given and possible reasons for this settlement over 150 years is analyzed.
Fibroadenoma of the breast; incidence of malignancy and indicators for surgical intervention: An analysis of 1392 patients
Authors: Elnahas Waleed , Metwally Islam H. , Bonna Khaled , Youssef Marco , AbdAllah Samar , Bonna Mohamed , Ali Faried Mohamed , Atef Tira Mohamed , Hamdy Omar 
Published: December 2022

BACKGROUND: Fibroadenomas are common among young females. The size of the lesion used to be an indication for further assessment or excision. With arising of the watch and see proponents, criteria for selecting patients are important to establish. METHODS: This is a retrospective study of a prospectively maintained database where all patients having the clinical/radiological provisional diagnosis of fibroadenoma and attending our center - from January 2008 to March 2020 - were enrolled. The primary outcome was the incidence of malignancy and the secondary outcomes were the correlation of malignancy-risk with epidemiologic and radiologic criteria. RESULTS: The study enrolled 1392 patients. The mean age of the patients was 35.7 + ∕− 13.1 years. The median of the longest diameter of the detected breast lesions was 25 mm. The incidence of malignancy was 188 (13.5%). The size of the lesion measured by largest diameter was insignificant (p = 0.99), while the patients’ age, marital status, and imaging criteria as measured by BIRADS score were significant (<0.001). CONCLUSION: Approaching patients with the age above 35 or with BIRADS 4 provisionally diagnosed with fibroadenomas should be cautious with biopsy and short-term follow-ups The size of the tumor alone should not be used as an indication for surgical intervention.
Biological age and diet: Measuring the impact of lifestyle on a 6CpG-epigenetic clock
Authors: Bordoni Laura , Malinowska Anna M. , Petracci Irene , Chmurzynska Agata , Gabbianelli Rosita 
Published: December 2022

BACKGROUND: Changes in DNA methylation along the life have been documented, and environmental exposures (including diet, physical activity and smoking) can accelerate or decelerate this process. The epigenetic clock estimates the biological age of an individual measuring methylation patterns in specific areas of its genome. Recently, a new epigenetic clock based on 6 CpGs has been proposed, with high potential to become an easy accessible tool able to measure the epigenetic age (EA) of an individual. OBJECTIVES: This study aims to validate the 6 CpG epigenetic clock comparing it with other biomarkers of aging such as telomere length (TL) and methylation in the long interspersed nuclear element-1 (LINE-1). The impact of lifestyle associated factors on these molecular marks has been evaluated. METHODS: 200 healthy participants having extreme dietary patterns (healthy vs western diet) were selected. Dietary intakes, body composition, physical activity level and smoking has been assessed. DNA extracted from whole blood was used to measure the 6CpG-EA, TL and LINE-1 methylation levels. RESULTS: 6CpG-EA was positively correlated with chronological age (r = 0.591; p = 7.2*10-20) and negatively with TL (r = –0.150; p = 0.040) and LINE-1 methylation (r = –0.240; p = 0.001). Despite the fact that no significant associations were detected with the overall diet quality (HEI), 6CpG-EA was correlated with dietary intakes of nutrients involved in the one-carbon (1 C) metabolism, especially in the western diet group. CONCLUSION: These results support the 6CpG epigenetic clock as an easy accessible tool to estimate biological age, in accordance with other molecular markers of aging, and suggest that EA can be modulated by micronutrients involved in the 1 C metabolism.
A Study to Assess the Effectiveness of Pelvic Floor Muscle Strengthening Exercises on Erectile Dysfunction in Rectal Cancer Survivors at Tertiary Cancer Hospital, TMH, Mumbai
Authors: Jagdiish Prathepa , Shirke Amit , Saklani Avanish , Kazi Muffadal , Engineer Reena , Daptardar Anuradha , Goswami Savita 
Published: December 2022

OBJECTIVES: ―A study to assess the effectiveness of pelvic floor muscle strengthening exercises on erectile dysfunction in rectal cancer survivors at tertiary cancer hospital, TMH, Mumbai.

METHODOLOGY: Single arm prospective study approach was used for the study. It was considered to be the most suitable method here. The research design used for the present study was self-controlled trial design. The desired sample for the present study was 39 participants. The proposed sample size was 39 for approval to regulatory authorities. The sample selected were 39 participants who will be receiving an intervention of pelvic floor muscle strengthening exercises in the selected areas of Tata Memorial Hospital. Data gathered was analyzed using descriptive and inferential statistics.

RESULTS OF THE STUDIES: For this study 39 participants were enrolled. All participants had filled pre-intervention SHIM questionnaire having 5 different questions in terms of Confidence, erection, penetration, difficulty and satisfaction. Patients had been taught Pelvic floor muscle strengthening exercises on Day 0, which were asked to carry out 3 times a day for a period of 10 weeks. After 10 weeks of exercises , patient had taken for Post- intervention SHIM score of all 39 participants. In pre-intervention SHIM questionnaire, Mean score and Standard Deviation among 5 questions were Confidence (2.6154±0.5901), Erection (2.5128±0.6014), Penetration (2.1764±0.3888) , Difficulty(2.2564±0.5964) and satisfaction(2.2821±0.5595). In Post intervention SHIM score among 5 questions were Confidence (3.0513±0.6047), Erection(2.8974±0.6804), Penetration(2.3590±0.6277), Difficulty(2.3590±0.6277) and satisfaction(2.6410±0.6684) The confidence, erection, satisfaction post intervention were statistically highly significant in participants (P< 0.001) and in term of Difficulty it was statistically significant (P 0.005).

The Wilcoxon signed rank test explains that there was a significant increase seen in the scores of confidence (4.123), erection (3.873) and satisfaction (3.500). They were highly statistically significant (P< 0.001).

There was a significant increase seen in the scores of penetration (4.123) and difficulty (3.873). They were statistically significant (p 0.001 and 0.046) respectively. Hence the pelvic floor muscle strengthening exercises were helpful in improving erectile dysfunction among rectal cancer survivors.

CONCLUSION: It could be concluded that null hypothesis H0 is rejected and H1 hypothesis is accepted. Pelvic floor muscle strengthening exercises in rectal cancer survivors helped to improve erectile dysfunction among men. As the study is done for 10 weeks. The result for better outcome in terms of penetration and difficulty needs monitoring or follow up to 6 months with continuous exercises taught to patient
Regular Consumption of Lacto-fermented Vegetables has Greater Effects on the Gut Metabolome Compared with the Microbiome
Authors: Guse Kylene , Sharma Ashok , Weyenberg Emily , Davison Sam , Ma Yiwei , Choi Yuni , Johnson Abigail , Chen Chi , Gomez Andres 
Published: December 2022

The industrialization of Western food systems has greatly reduced the regular consumption of lacto-fermented vegetables (LFV). Consuming LFV may exert health benefits through the alteration of the gut microbiome, but the events and mechanisms involved remain unclear. To start understanding the possible benefits of LFV, we compared fecal microbial diversity and composition, as well as dietary habits between individuals who regularly consume LFV (n=23) and those who do not (n=24). We utilized microbial DNA amplicon sequencing (16s rRNA and ITS2) and untargeted metabolomics (LC-MS) to analyze stool samples. Study participants also provided three consecutive days of dietary data. Results show only minor effects on microbiome composition; with the enrichment of a few microorganisms potentially associated with vegetable ferments, such as _Leuconostoc mesenteroides and Rhodotorula mucilaginosa (P<0.05)_, in LFV consumers. However, LFV consumption had greater effects on the fecal metabolome, with higher abundances of butyrate, acetate, and valerate (P<0.05) and significantly greater metabolome diversity (_P<0.001_). Overall, the observations of minor changes in the fecal microbiome and greater effects on the fecal metabolome from LFV consumption warrant further investigations on the health significance of LFV as regular components of the daily diet in humans.
FACIAL REDUCTION ACROSS THREE REPRESENTATIVE TAXA: A GEOMETRIC MORPHOMETRIC APPROACH
Authors: Selba Molly Chisholm 
Published: December 2022

Facial reduction is a phenomenon described extensively in the evolutionary, clinical, and veterinary literature. Despite the fact that this phenomenon applies to a variety of species and can even be found in the clinical medical and veterinary literature (midfacial hypoplasia, craniosynostosis conditions, etc.), it has not been analyzed comprehensively using a geometric morphometric approach. The goal of this study was to improve our understanding of facial reduction as both an evolutionary process and as the result of selective breeding. Three representative taxa (bats, primates, and dogs) were selected for this project for their presence of both a normocephalic and brachycephalic morphotype. Bats and primates evolved their facial reduction as the result of natural selection and other evolutionary processes over a long period of time with a large amount of genetic diversity. Dogs, on the other hand, developed their squished-face morphotype over the space of a few thousand years. Despite this fact, dogs have been proposed as a model for evolutionary facial reduction. Although dogs were able to achieve similar—if not more pronounced—facial reduction, they did so in a completely different way than bats and dogs.
This project included the analysis of specific patterns of localized shape change across these representative three taxa. The impact of this facial reduction on the soft tissue structures of the skull—namely, the brain—was assessed through the study of canine endocasts. We analyzed all three taxa through the analysis of six previously-established modules. These modules include the six main areas proposed by Goswami (2006): face, orbit, oral, zygomatic, cranial vault and cranial base. Modularity and integration were evaluated for these modules in order to better understand the constraints present during facial reduction. Finally, both global and modular facial reduction were analyzed in the three representative taxa using geometric morphometric analysis. Despite being proposed as a model of evolutionary facial reduction, dogs demonstrate major differences in cranial morphology as the result of selective breeding processes. Furthermore, facial reduction that is the result of evolutionary processes is not consistent between bats and primates, and instead, bats share much of their cranial morphology in common with dogs. Overall, it is clear that facial reduction is a multi-faceted morphological phenomenon of evolutionary significance.
Propaedeutics of programming: how to prepare students for the perception of object-oriented programming
Authors: Gerbut S. S. 
Published: December 2022

The article describes the methodological foundations for preparing for programming in basic school and, in particular, for the perception of object-oriented programming in the future. This methodics can be implemented in the course of extracurricular activities or use its fragments for implementation in the lesson. Programming is one of the most important sections in the basic school informatics course. This is an interesting and at the same time quite difficult section for initial learning. Given that the basic informatics course starts in the seventh grade and is only one hour per week (advanced — two hours per week), programming is allocated only in the eighth grade and not so much. And you need to introduce pupils to a large number of concepts, teach them how to write programs, from linear programs to array processing. In this regard, it is proposed to use a propaedeutic course of extracurricular activity for students of the sixth-seventh grades in order to familiarize them with the basics of structured programming using the example of working with performers, which allow them to realize familiarity with the structure of the program, syntactic requirements, basic algorithmic constructions through drawing and movement of graphic objects (according the teacher's choice — in algorithmic language, PascalABC, Python, C++). The methodological developments described in the article can be used for those performers who will not be in the lessons, or, conversely, to introduce students to writing programs in a language that will be studied further in the lessons, which will make it easier for them to perceive the material on programming in basic school and develop their abilities in this direction along an individual educational trajectory — both in the classroom and in extracurricular activities.