bluuebunny/crossref_metadata_2025_split huggingface dataset:
Effects of S-Adenosylmethionine on Cognition in Animals and Humans: A Systematic Review and Meta-Analysis of Randomized Controlled Trials
Authors: Zhao Yan , Zhang Yizhou , Meng Sijia , Chen Bingyu , Dong Xinyi , Guo Xiaojing , Guo Fangzhen , Zhang Runjiao , Cui Huixian , Li Sha 
Published: March 2023

Background: There is increasing evidence that supplementation of S-adenosylmethionine (SAM) can improve cognitive function in animals and humans, although the outcomes are not always inconsistent. Objective: We conducted a systematic review and meta-analysis to evaluate the correlation between SAM supplementation and improved cognitive function. Methods: We searched studies in the PubMed, Cochrane Library, Embase, Web of Science, and Clinical Trials databases from January 1, 2002 to January 1, 2022. Risk of bias was assessed using the Cochrane risk of bias 2.0 (human studies) and the Systematic Review Center for Laboratory Animal Experimentation risk of bias (animal studies) tools; and evidence quality was evaluated using the Grading of Recommendations Assessment, Development, and Evaluation. STATA software was employed to perform meta-analysis, and the random-effects models was used to evaluate the standardized mean difference with 95% confidence intervals. Results: Out of the 2,375 studies screened, 30 studies met the inclusion criteria. Meta-analyses of animal ( p = 0.213) and human ( p = 0.047) studies showed that there were no significant differences between the SAM supplementation and control groups. The results of the subgroup analyses showed that the animals aged ≤8 weeks ( p = 0.027) and the intervention duration >8 weeks ( p = 0.009) were significantly different compared to the controls. Additionally, the Morris water maze test ( p = 0.005) used to assess the cognitive level of the animals revealed that SAM could enhance spatial learning and memory in animals. Conclusion: SAM supplementation showed no significant improvement in cognition. Therefore, further studies are needed to assess the effectiveness of SAM supplementation.
Ландшафтно-рекреаційна оцінка рекреаційно-оздоровчих лісів Лівобережної України
Authors: Мусієнко С. І. , Тарнопільська О. М. , Бондаренко В. В. , Лук’янець В. А. , Кобець О. В. , Костяшкіна Т. Д. 
Published: March 2023

На основі аналізу повидільної таксаційної бази даних «Лісовий фонд» ВО «Укрдержліспроект», матеріалів лісовпорядкування та з урахуванням природних особливостей місцевості й цільового призначення лісових насаджень здійснено ландшафтно-рекреаційне оцінювання рекреаційно-оздоровчих лісів Лівобережної України. Виявлено, що вони характеризуються порівняно невисоким ступенем рекреаційного навантаження. В рекреаційно-оздоровчих лісах Лівобережної України переважає закритий тип ландшафту, частка площі якого становить 85,1 %. За естетичною оцінкою переважна більшість таких лісів належать до другого та третього класів, середній клас становить 2,5 бала. Невисока естетична оцінка рекреаційно-оздоровчих лісів Лівобережної України пов’язана з переважанням чистих за складом деревостанів, а також таких, що сформовані з аборигенних деревних видів. Покращити естетичну оцінку насаджень можливо шляхом уведення рідкісних або інтродукованих видів, які б підвищували контрастність пейзажів.
A predictive model for early clinical diagnosis of spinal tuberculosis based on conventional laboratory indices: A multicenter real-world study
Authors: Hu Xiaojiang , Zhang Guang , Zhang Hongqi , Tang Mingxing , Liu Shaohua , Tang Bo , Xu Dongcheng , Zhang Chengran , Gao Qile 
Published: March 2023

BackgroundEarly diagnosis of spinal tuberculosis (STB) remains challenging. The aim of this study was to develop a predictive model for the early diagnosis of STB based on conventional laboratory indicators.MethodThe clinical data of patients with suspected STB in four hospitals were included, and variables were screened by Lasso regression. Eighty-five percent of the cases in the dataset were randomly selected as the training set, and the other 15% were selected as the validation set. The diagnostic prediction model was established by logistic regression in the training set, and the nomogram was drawn. The diagnostic performance of the model was verified in the validation set.ResultA total of 206 patients were included in the study, including 105 patients with STB and 101 patients with NSTB. Twelve variables were screened by Lasso regression and modeled by logistic regression, and seven variables (TB.antibody, IGRAs, RBC, Mono%, RDW, AST, BUN) were finally included in the model. AUC of 0.9468 and 0.9188 in the training and validation cohort, respectively.ConclusionIn this study, we developed a prediction model for the early diagnosis of STB which consisted of seven routine laboratory indicators.
Identification of vulnerable non-culprit lesions by coronary computed tomography angiography in patients with chronic coronary syndrome and diabetes mellitus
Authors: Zhao Jia , Zhang Hong , Liu Chang , Zhang Ying , Xie Cun , Wang Minghui , Wang Chengjian , Wang Shuo , Xue Yuanyuan , Liang Shuo , Gao Yufan , Cong Hongliang , Li Chunjie , Zhou Jia 
Published: March 2023

BackgroundAmong patients with diabetes mellitus (DM) and chronic coronary syndrome (CCS), non-culprit lesions (NCLs) are responsible for a substantial number of future major adverse cardiovascular events (MACEs). Thus, we aimed to establish the natural history relationship between adverse plaque characteristics (APCs) of NCLs non-invasively identified by coronary computed tomography angiography (CCTA) and subsequent MACEs in these patients.MethodsBetween January 2016 and January 2019, 523 patients with DM and CCS were included in the present study after CCTA and successful percutaneous coronary intervention (PCI). All patients were followed up for MACEs (the composite of cardiac death, myocardial infarction, and unplanned coronary revascularization) until January 2022, and the independent clinical event committee classified MACEs as indeterminate, culprit lesion (CL), and NCL-related. The primary outcome was MACEs arising from untreated NCLs during the follow-up. The association between plaque characteristics detected by CCTA and primary outcomes was determined by Marginal Cox proportional hazard regression.ResultsOverall, 1,248 NCLs of the 523 patients were analyzed and followed up for a median of 47 months. The cumulative rates of indeterminate, CL, and NCL-related MACEs were 2.3%, 14.5%, and 20.5%, respectively. On multivariate analysis, NCLs associated with recurrent MACEs were more likely to be characterized by a plaque burden &gt;70% [hazard ratio (HR), 4.35, 95% confidence interval (CI): 2.92–6.44], a low-density non-calcified plaque (LDNCP) volume &gt;30 mm3 (HR: 3.40, 95% CI: 2.07–5.56), a minimal luminal area (MLA) &lt;4 mm2 (HR: 2.30, 95% CI: 1.57–3.36), or a combination of three APCs (HR: 13.69, 95% CI: 9.34–20.12, p &lt; 0.0001) than those not associated with recurrent MACEs. Sensitivity analysis regarding all indeterminate MACEs as NCL-related ones demonstrated similar results.ConclusionsIn DM patients who presented with CCS and underwent PCI, half of the MACEs occurring during the follow-up were attributable to recurrence at the site of NCLs. NCLs responsible for unanticipated MACEs were frequently characterized by a large plaque burden and LDNCP volume, a small MLA, or a combination of these APCs, as determined by CCTA.
Study on the restraint control of an isolation pile on an existing high-speed railway during the close passing of a shield machine
Authors: Tao Yang , Shuailei Liu , Hao Zhao , Xingru Li 
Published: March 2023

The impact of shield construction on the close lateral passing of high-speed railways can threaten the safety of operating high-speed railways. To study the deformation characteristics of the shield structure after the application of isolation piles from the ground surface and the deformation characteristics of the bridge piers of an overpassing high-speed railway, we analyze the mechanism of isolation pile restraint control and establish a three-dimensional numerical model of a shield structure to study the situation of a high-speed railway with a metro interval under passing a high-speed railway passenger line project. Combined with the measured data and numerical simulation data of the same construction stage, we study the impact of shield structure lateral passing on high-speed railway piers and surrounding ground surface in terms of the disturbance changes and the effect of isolation, as well as the effect of the lateral passing of the shield tunnel on the surface of the bridge piers and the surrounding area and the control effect of the isolation piles on the deformation. The results show that the isolation pile can block the development of the soil layer rupture surface and the stress path. The isolation pile has a good control effect on the vertical displacement of the bridge pier. The vertical displacement of the bridge pier decreases from 0.6 mm to 1.1 mm after the single- and double-track tunneling, and the displacement changes along the depth direction from non-uniform to relatively uniform. The vertical displacement of the ground surface includes both uplift and settlement, which occur at the same time. When the shield tunneling advances, the form of the ground settlement trough is changed from a “V” shape formed by the right single-track tunneling to a “W” shape after the double-track tunneling. The isolation pile can effectively control the influence range of tunnel excavation; its lateral displacement form changes regularly, and the lateral uplift is always in a “double-C outward expansion” shape. The initial excavation greatly disturbs the initial stress field, so it is necessary to pay attention to the control and management of measures to destroy the initial stress field.
Monogenic diabetes in New Zealand - An audit based revision of the monogenic diabetes genetic testing pathway in New Zealand
Authors: Harrington Francesca , Greenslade Mark , Colclough Kevin , Paul Ryan , Jefferies Craig , Murphy Rinki 
Published: March 2023

AimsTo evaluate (a) the diagnostic yield of genetic testing for monogenic diabetes when using single gene and gene panel-based testing approaches in the New Zealand (NZ) population, (b) whether the MODY (Maturity Onset Diabetes of the Young) pre-test probability calculator can be used to guide referrals for testing in NZ, (c) the number of referrals for testing for Māori/Pacific ethnicities compared to NZ European, and (d) the volume of proband vs cascade tests being requested.MethodsA retrospective audit of 495 referrals, from NZ, for testing of monogenic diabetes genes was performed. Referrals sent to LabPlus (Auckland) laboratory for single gene testing or small multi-gene panel testing, or to the Exeter Genomics Laboratory, UK, for a large gene panel, received from January 2014 – December 2021 were included. Detection rates of single gene, small multi-gene and large gene panels (neonatal and non-neonatal), and cascade testing were analysed. Pre-test probability was calculated using the Exeter MODY probability calculator and ethnicity data was also collected.ResultsThe diagnostic detection rate varied across genes, from 32% in GCK, to 2% in HNF4A, with single gene or small gene panel testing averaging a 12% detection rate. Detection rate by type of panel was 9% for small gene panel, 23% for non-neonatal monogenic diabetes large gene panel and 40% for neonatal monogenic diabetes large gene panel. 45% (67/147) of patients aged 1-35 years at diabetes diagnosis scored &lt;20% on MODY pre-test probability, of whom 3 had class 4/5 variants in HNF1A, HNF4A or HNF1B. Ethnicity data of those selected for genetic testing correlated with population diabetes prevalence for Māori (15% vs 16%), but Pacific People appeared under-represented (8% vs 14%). Only 1 in 6 probands generated a cascade test.ConclusionsA new monogenic diabetes testing algorithm for NZ is proposed, which directs clinicians to choose a large gene panel in patients without syndromic features who score a pre-test MODY probability of above 20%.
The association between total bile acid and bone mineral density among patients with type 2 diabetes
Authors: Yang Song , Li Hongyun , Gu Yuanyuan , Wang Qiang , Dong Li , Xu Chao , Fan Yuxin , Liu Ming , Guan Qingbo , Ma Lixing 
Published: March 2023

ObjectiveBile acids have underlying protective effects on bones structure. Long-term diabetes also causes skeletal disorders including osteoporosis, Charcot arthropathy and renal osteodystrophy. Nevertheless, few studies have reported whether bile acid is associated with bone metabolism in diabetics. This study aimed to explore the relationship between total bile acid (TBA) and bone mineral density (BMD) among patients with type 2 diabetes mellitus (T2DM).MethodsWe retrospectively included 1,701 T2DM patients who were hospitalized in Taian City Central Hospital (TCCH), Shandong Province, China between January 2017 to December 2019. The participants were classified into the osteopenia (n = 573), osteoporosis (n= 331) and control groups (n= 797) according to BMD in the lumbar spine and femoral. The clinical parameters, including TBA, bilirubin, vitamin D, calcium, phosphorus and alkaline phosphatase were compared between groups. Multiple linear regression was used to analyze the relationship between TBA and BMD in lumbar spine, femoral, trochiter, ward’s triangle region. A logistic regression was conducted to develop a TBA-based diagnostic model for differentiating abnormal bone metabolism from those with normal BMD. We evaluated the performance of model using ROC curves.ResultsThe TBA level was significantly higher in patients with osteoporosis (Median[M]= 3.300 μmol/L, interquartile range [IQR] = 1.725 to 5.250 μmol/L) compared to the osteopenia group (M = 3.200 μmol/L, IQR = 2.100 to 5.400 μmol/L) and control group (M = 2.750 μmol/L, IQR = 1.800 to 4.600 μmol/L) (P &lt;0.05). Overall and subgroup analyses indicated that TBA was negatively associated with BMD after adjusted for the co-variates (i.e., age, gender, diabetes duration, BMI, total bilirubin, direct bilirubin, indirect bilirubin) (P &lt;0.05). Logistic regression revealed that higher TBA level was associated with increased risk for abnormal bone metabolism (OR = 1.044, 95% CI = 1.005 to 1.083). A TBA-based diagnostic model was established to identify individuals with abnormal bone metabolism (T-score ≤ -1.0). The area under ROC curve (AUC) of 0.767 (95% CI = 0.730 to 0.804).ConclusionOur findings demonstrated the potential role of bile acids in bone metabolism among T2DM patients. The circulating TBA might be employed as an indicator of abnormal bone metabolism.
Global-feature of autoimmune glomerulonephritis using proteomic analysis of laser capture microdissected glomeruli
Authors: Dong Jingjing , Zheng Fengping , Liu Fanna , He Jingquan , Li Shanshan , Pu Wenjun , Xu Huixuan , Luo Zhifeng , Liu Shizhen , Yin Lianghong , Tang Donge , Dai Yong 
Published: March 2023

BackgroundIgA nephropathy (IgAN), (LN), membranous nephropathy (MN), and minimal change nephropathy (MCN) are all belonged to autoimmune glomerulonephritis. This study aimed to identify the specific proteomic characteristics of the four GNs diseases in order to provide frameworks for developing the appropriate drug for patients diagnosed with GNs disease.MethodsLiquid chromatography−tandem mass spectrometry (LC-MS/MS) was utilized to investigate proteomic features of glomerular tissues obtained by laser capture microdissection (LCM). 8 normal control cases, 11 IgAN cases, 19 LN cases, 5 MN cases, and 3 MCN cases in this study were selected for bioinformatics analyses.ResultsThe shared overlapping proteins among the top 100 DEPs of each GNs type were mostly downregulated, in which only FLII was significantly downregulated in the four GNs diseases. A2M was significantly upregulated in MN, IgAN, and LN subgroups. The pathway of complement and coagulation cascades was notably activated with NES value ranging 2.77 to 3.39 among MCN, MN, IgAN, and LN diseases, but the pattern of protein expression level were significantly different. In LN patients, the increased activity of complement and coagulation cascades was contributed by the high expression of multiple complements (C1QB, C3, C4A, C4B, C6, C8B, C8G, C9). Meanwhile, both C1QC and C4B were remarkably upregulated in MN patients. On the contrary, complement-regulating proteins (CD59) was substantially decreased in MCN and IgAN subgroup.ConclusionsThe integrative proteomics analysis of the four GNs diseases provide insights into unique characteristics of GNs diseases and further serve as frameworks for precision medicine diagnosis and provide novel targets for drug development.
Immune profiles according to EGFR mutant subtypes and correlation with PD-1/PD-L1 inhibitor therapies in lung adenocarcinoma
Authors: Koh Young Wha , Park Bumhee , Jung Se Hee , Han Jae-Ho , Haam Seokjin , Lee Hyun Woo 
Published: March 2023

BackgroundWe examined the distributions of 22 immune cell types and the responses to PD-1/PD-L1 inhibitors according to EGFR mutation profile, in three independent datasets of lung adenocarcinoma (LUAD).MethodsWe used CIBERSORTx to analyze the distributions of immune cells, and tumor immune dysfunction and exclusion (TIDE) or tumor mutation burden (TMB) to analyze responses to anti-PD-1/PD-L1 therapy, in two public LUAD datasets. The results were verified with a validation set that included patients treated with PD-1/PD-L1 inhibitors.ResultsCompared to EGFR mutants, EGFR wild-type carcinomas had higher numbers of CD8+ T cells, CD4 memory activated T cells and neutrophils, and lower numbers of resting dendritic cells and resting mast cells, in two of the datasets. In our subgroup analyses, CD8+ T cells and CD4 memory activated T cells were more numerous in EGFR rare variants than in wild-types, L858R mutants, and exon 19 deletion mutants. In our TIDE or TMB analyses, EGFR rare variants were predicted to respond better to PD-1/PD-L1 inhibitors than wild-types, L858R mutants, and exon 19 deletion mutants. In the validation set verified by immunohistochemical staining, levels of CD8+ T cells in the EGFR rare variant or wild-type groups were significantly higher than in the EGFR L858R and exon 19 deletion groups. In patients treated with PD-1/PD-L1 inhibitors, the survival rates of patients with EGFR wild-type and rare mutant carcinomas were higher than those with L858R and exon 19 deletion carcinomas.ConclusionThe EGFR rare mutation form of LUAD shows a higher immune activation state compared to wild-type, L858R, and exon 19 deletion variants, indicating it as a potential target for PD-1/PD-L1 inhibitor therapy.
Microglial activation persists beyond clinical recovery following sport concussion in collegiate athletes
Authors: Neumann Kiel D. , Seshadri Vikram , Thompson Xavier D. , Broshek Donna K. , Druzgal Jason , Massey James C. , Newman Benjamin , Reyes Jose , Simpson Spenser R. , McCauley Katelyenn S. , Patrie James , Stone James R. , Kundu Bijoy K. , Resch Jacob E. 
Published: March 2023

IntroductionIn concussion, clinical and physiological recovery are increasingly recognized as diverging definitions. This study investigated whether central microglial activation persisted in participants with concussion after receiving an unrestricted return-to-play (uRTP) designation using [18F]DPA-714 PET, an in vivo marker of microglia activation.MethodsEight (5 M, 3 F) current athletes with concussion (Group 1) and 10 (5 M, 5 F) healthy collegiate students (Group 2) were enrolled. Group 1 completed a pre-injury (Visit1) screen, follow-up Visit2 within 24 h of a concussion diagnosis, and Visit3 at the time of uRTP. Healthy participants only completed assessments at Visit2 and Visit3. At Visit2, all participants completed a multidimensional battery of tests followed by a blood draw to determine genotype and study inclusion. At Visit3, participants completed a clinical battery of tests, brain MRI, and brain PET; no imaging tests were performed outside of Visit3.ResultsFor Group 1, significant differences were observed between Visits 1 and 2 (p &lt; 0.05) in ImPACT, SCAT5 and SOT performance, but not between Visit1 and Visit3 for standard clinical measures (all p &gt; 0.05), reflecting clinical recovery. Despite achieving clinical recovery, PET imaging at Visit3 revealed consistently higher [18F]DPA-714 tracer distribution volume (VT) of Group 1 compared to Group 2 in 10 brain regions (p &lt; 0.001) analyzed from 164 regions of the whole brain, most notably within the limbic system, dorsal striatum, and medial temporal lobe. No notable differences were observed between clinical measures and VT between Group 1 and Group 2 at Visit3.DiscussionOur study is the first to demonstrate persisting microglial activation in active collegiate athletes who were diagnosed with a sport concussion and cleared for uRTP based on a clinical recovery.
Leptin deficiency-caused behavioral change – A comparative analysis using EthoVision and DeepLabCut
Authors: Bühler Daniel , Power Guerra Nicole , Müller Luisa , Wolkenhauer Olaf , Düffer Martin , Vollmar Brigitte , Kuhla Angela , Wolfien Markus 
Published: March 2023

IntroductionObese rodents e.g., the leptin-deficient (ob/ob) mouse exhibit remarkable behavioral changes and are therefore ideal models for evaluating mental disorders resulting from obesity. In doing so, female as well as male ob/ob mice at 8, 24, and 40 weeks of age underwent two common behavioral tests, namely the Open Field test and Elevated Plus Maze, to investigate behavioral alteration in a sex- and age dependent manner. The accuracy of these tests is often dependent on the observer that can subjectively influence the data.MethodsTo avoid this bias, mice were tracked with a video system. Video files were further analyzed by the compared use of two software, namely EthoVision (EV) and DeepLabCut (DLC). In DLC a Deep Learning application forms the basis for using artificial intelligence in behavioral research in the future, also with regard to the reduction of animal numbers.ResultsAfter no sex and partly also no age-related differences were found, comparison revealed that both software lead to almost identical results and are therefore similar in their basic outcomes, especially in the determination of velocity and total distance movement. Moreover, we observed additional benefits of DLC compared to EV as it enabled the interpretation of more complex behavior, such as rearing and leaning, in an automated manner.DiscussionBased on the comparable results from both software, our study can serve as a starting point for investigating behavioral alterations in preclinical studies of obesity by using DLC to optimize and probably to predict behavioral observations in the future.