bluuebunny/crossref_metadata_2025_split huggingface dataset:
Application of Image Processing in Evaluation of Hydraulic Fracturing with Liquid Nitrogen: A Case Study of Coal Samples from Karaganda Basin
Authors: Longinos Sotirios Nik. , Abbas Azza Hashim , Bolatov Arman , Skrzypacz Piotr , Hazlett Randy 
Published: July 2023

Research of microstructure and permeability evolution of coal following LN2 treatment elucidate the process of cryogenic fracturing due to environmentally friendly behavior in comparison with conventional hydraulic fracturing. The evolution of the 2D microstructure of bituminous coal before and after LN2 treatment was examined using a high-resolution camera. The image processing was implemented using functions from the OpenCV Python library that are sequentially applied to digital images of original coal samples. The images were converted into binary pixel matrices to identify cracks and to evaluate the number of cracks, crack density, total crack area, and average crack length. Results were visualized using Seaborn and Matplotlib Python libraries. There were calculations of total crack area (TCA), total number of cracks (TNC), crack density (CD), the average length of cracks (Q2), first (Q1) and third (Q3) quartiles in fracture length statistics. Our findings demonstrate a progressive increase in the Total Crack Area (δTCA) with longer freezing times and an increased number of freezing–thawing cycles. In contrast, the change in crack density (δCD) was generally unaffected by freezing time alone but exhibited a significant increase after several freezing–thawing cycles. Among the freezing times investigated, the highest crack density (CD) value of 300 m−1 was achieved in FT60, while the lowest CD value of 31.25 m−1 was observed in FT90 after liquid nitrogen (LN2) treatment. Additionally, the FTC4 process resulted in a 50% augmentation in the number of cracks, whereas the FTC5 process tripled the number of small cracks.
Cloning, Identification, and Functional Analysis of the Chalcone Isomerase Gene from Astragalus sinicus
Authors: Zhang Xian , Xu Jing , Si Linlin , Cao Kai , Wang Yuge , Li Hua , Wang Jianhong 
Published: July 2023

Astragalus sinicus is an important winter-growing cover crop. It is widely utilized, not only as a cover crop for its benefits in fertilizing the soil but also as a landscape ground cover plant. Anthocyanins are involved in the pigmentation of plants in leaves and flowers, which is a crucial characteristic trait for A. sinicus. The formation of anthocyanins depends significantly on the enzyme chalcone isomerase (CHI). However, research on the CHI gene of A. sinicus remains unexplored. The rapid amplification of cDNA ends (RACE) approach was used in this research to clone the CHI sequence from A. sinicus (AsiCHI). The expression profiles of the AsiCHI gene in multiple tissues of A. sinicus were subsequently examined by qRT-PCR (Quantitative Real-Time PCR). Furthermore, the function of the AsiCHI was identified by the performance of ectopic expression in Arabidopsis (Arabidopsis thaliana). The outcomes revealed that the full-length cDNA of the AsiCHI gene (GeneBank: OQ870547) measured 972 bp in length and included an open reading frame of 660 bp. The encoded protein contains 219 amino acids with a molecular weight of 24.14 kDa and a theoretical isoelectric point of 5.11. In addition, the remarkable similarity between the AsiCHI protein and the CHI proteins of other Astragalus species was demonstrated by the sequence alignment and phylogenetic analysis. Moreover, the highest expression level of AsiCHI was observed in leaves and showed a positive correlation with anthocyanin content. The functional analysis further revealed that the overexpression of AsiCHI enhanced the anthocyanidin accumulation in the transgenic lines. This study provided a better understanding of AsiCHI and elucidated its role in anthocyanin production.
Bone Morphogenetic Protein 13 Has Protumorigenic Effects on Hepatocellular Carcinoma Cells In Vitro
Authors: Kersten Vanessa , Seitz Tatjana , Sommer Judith , Thasler Wolfgang E. , Bosserhoff Anja , Hellerbrand Claus 
Published: July 2023

Activated hepatic stellate cells (HSCs) play a key role in hepatic fibrosis and, thus, build the “soil” for hepatocarcinogenesis. Furthermore, HSCs are known to promote the progression of hepatocellular carcinoma (HCC), but the molecular mechanisms are only incompletely understood. Recently, we newly described the expression of bone morphogenetic protein 13 (BMP13) by HSCs in fibrotic liver tissue. In addition, BMP13 has mostly been studied in the context of cartilage and bone repair, but not in liver disease or cancer. Thus, we aimed to analyze the expression and function of BMP13 in HCC. Expression analyses revealed high BMP13-expression in activated human HSCs, but not in human HCC-cell-lines. Furthermore, analysis of human HCC tissues showed a significant correlation between BMP13 and α-smooth muscle actin (α-SMA), and immunofluorescence staining confirmed the co-localization of BMP13 and α-SMA, indicating activated HSCs as the cellular source of BMP13 in HCC. Stimulation of HCC cells with recombinant BMP13 increased the expression of the inhibitors of differentiation 1 (ID1) and 2 (ID2), which are known targets of BMP-signaling and cell-cycle promotors. In line with this, BMP13-stimulation caused an induced SMAD 1/5/9 and extracellular signal-regulated kinase (ERK) phosphorylation, as well as reduced expression of cyclin-dependent kinase inhibitors 1A (CDKN1A) and 2A (CDKN2A). Furthermore, stimulation with recombinant BMP13 led to increased proliferation and colony size formation of HCC cells in clonogenicity assays. The protumorigenic effects of BMP13 on HCC cells were almost completely abrogated by the small molecule dorsomorphin 1 (DMH1), which selectively blocks the intracellular kinase domain of ALK2 and ALK3, indicating that BMP13 acts via these BMP type I receptors on HCC cells. In summary, this study newly identifies stroma-derived BMP13 as a potential new tumor promotor in HCC and indicates this secreted growth-factor as a possible novel therapeutic target in HCC.
Improving Rice Leaf Shape Using CRISPR/Cas9-Mediated Genome Editing of SRL1 and Characterizing Its Regulatory Network Involved in Leaf Rolling through Transcriptome Analysis
Authors: Han Yue , Yang Jinlian , Wu Hu , Liu Fang , Qin Baoxiang , Li Rongbai 
Published: July 2023

Leaf rolling is a crucial agronomic trait to consider in rice (Oryza sativa L.) breeding as it keeps the leaves upright, reducing interleaf shading and improving photosynthetic efficiency. The SEMI-ROLLED LEAF 1 (SRL1) gene plays a key role in regulating leaf rolling, as it encodes a glycosylphosphatidylinositol-anchored protein located on the plasma membrane. In this study, we used CRISPR/Cas9 to target the second and third exons of the SRL1 gene in the indica rice line GXU103, which resulted in the generation of 14 T0 transgenic plants with a double-target mutation rate of 21.4%. After screening 120 T1 generation plants, we identified 26 T-DNA-free homozygous double-target mutation plants. We designated the resulting SRL1 homozygous double-target knockout as srl1-103. This line exhibited defects in leaf development, leaf rolling in the mature upright leaves, and a compact nature of the fully grown plants. Compared with the wild type (WT), the T2 generation of srl1-103 varied in two key aspects: the width of flag leaf (12.6% reduction compared with WT) and the leaf rolling index (48.77% increase compared with WT). In order to gain a deeper understanding of the involvement of SRL1 in the regulatory network associated with rice leaf development, we performed a transcriptome analysis for the T2 generation of srl1-103. A comparison of srl1-103 with WT revealed 459 differentially expressed genes (DEGs), including 388 upregulated genes and 71 downregulated genes. In terms of the function of the DEGs, there seemed to be a significant enrichment of genes associated with cell wall synthesis (LOC_Os08g01670, LOC_Os05g46510, LOC_Os04g51450, LOC_Os10g28080, LOC_Os04g39814, LOC_Os01g71474, LOC_Os01g71350, and LOC_Os11g47600) and vacuole-related genes (LOC_Os09g23300), which may partially explain the increased leaf rolling in srl1-103. Furthermore, the significant downregulation of BAHD acyltransferase-like protein gene (LOC_Os08g44840) could be the main reason for the decreased leaf angle and the compact nature of the mutant plants. In summary, this study successfully elucidated the gene regulatory network in which SRL1 participates, providing theoretical support for targeting this gene in rice breeding programs to promote variety improvement.
Isolation and Characterization of Poeciguamerin, a Peptide with Dual Analgesic and Anti-Thrombotic Activity from the Poecilobdella manillensis Leech
Authors: Wang Chaoming , Chen Mengrou , Lu Xiaoyu , Yang Shuo , Yang Min , Fang Yaqun , Lai Ren , Duan Zilei 
Published: July 2023

When Poecilobdella manillensis attacks its prey, the prey bleeds profusely but feels little pain. We and other research teams have identified several anticoagulant molecules in the saliva of P. manillensis, but the substance that produces the paralyzing effect in P. manillensis is not known. In this study, we successfully isolated, purified, and identified a serine protease inhibitor containing an antistasin-like domain from the salivary secretions of P. manillensis. This peptide (named poeciguamerin) significantly inhibited elastase activity and slightly inhibited FXIIa and kallikrein activity, but had no effect on FXa, trypsin, or thrombin activity. Furthermore, poeciguamerin exhibited analgesic activity in the foot-licking and tail-withdrawal mouse models and anticoagulant activity in the FeCl3-induced carotid artery thrombosis mouse model. In this study, poeciguamerin was found to be a promising elastase inhibitor with potent analgesic and antithrombotic activity for the inhibition of pain and thrombosis after surgery or in inflammatory conditions.
Pengaruh Ukuran Kuming Terhadap Pertumbuhan dan Hasil Tanaman Ubi Jalar (Ipomoea Batatas Lam.) di Distrik Manokwari Timur, Kabupaten Manokwari
Authors: Edowai Desi Natalia 
Published: July 2023

Penelitian ini bertujuan untuk mengetahui pengaruh ukuran kuming terhadap pertumbuhan dan hasil tanaman ubi jalar. Perlakuan dalam penelitian ini adalah kuming kecil dengan diameter 25 cm (P1), Kuming sedang dengan diameter 50 cm (P2), dan Kuming besar dengan diameter 100 cm (P3). Hasil penelitian menunjukan bahwa Ukuran kuming (kecil, sedang, dan besar) mempengaruhi pertumbuhan vegetatif (panjang sulur, jumlah sulur, dan berat brangkasan daun) dan generatif (berat brangkasan akar, berat akar pensil, berat akar serabut, jumlah akar pensil, dan panjang akar pensil) pada tanaman ubi jalar. Pada variable pengamatan panjang sulur kuming yang memberikan hasil tertinggi adalah kuming sedang (P2), sedangkan hasil tertinggi untuk jumlah sulur dan berat brangkasan daun terdapat pada perlakuan kuming kecil (P1). Pada penelitian ini tidak menghasilkan umbi (umbi kecil, umbi sedang, umbi besar), tetapi yang dihasilkan adalah umbi-umbi pensil dan terdapat banyak akar serabut. Kekurangan unsur hara dalam tanah terutama unsur kalium dan juga kemampuan adaptasi varietas yang rendah diduga merupakan faktor yang mempengaruhi hasil pertumbuhan vegetatif maupun pertumbuhan generatif tanaman ubi jalar.
Kata kunci : Kuming; Pertumbuhan dan Hasil Tanam; Ubi Jalar
Uji Mutu Kerupuk Ikan Gabus ( Channa Striata) Secara Organoleptik yang Diolah Kelompok Sentani Barokah di Kabupaten Jayapura
Authors: Jeujanan Samuel 
Published: July 2023

Ikan sebagai bahan pangan dapat diolah dalam beragam bentuk, salah satunya ialah kerupuk ikan. Sebagai produk yang disukai dan digemari oleh masyarakat di Kabupaten Jayapura yang mana sampai saat ini belum banyak informasi tentang mutu kerupuk ikan sehingga jaminan mutu dan keamanan pangan perlu diperhatikan. Tujuan penelitian ini ialah mengetahui mutu kerupuk ikan secara organoleptik terhadap kenampakan, bau, rasa, tekstur, dan jamur. Metode penelitian menggunakan petunjuk pengujian organoleptik dan atau sensori dari Badan Standarisasi Nasional Indonesia (SNI) Nomor. 01-2346-2006. Hasil penelitian dari 30 orang panelis menunjukkan bahwa rata-rata nilai kenampakan, bau, dan rasa sebesar 8,0. Sedangkan nilai tekstur dan jamur yaitu sebesar 9,0. Perolehan nilai tersebut menunjukkan bahwa mutu organoleptik kerupuk ikan gabus (Channa sp) yang diolah oleh kelompok Sentani Barokah binaan Dinas Perikanan Kabupaten Jayapura masih baik
A META 4 DO PLANO NACIONAL DE EDUCAÇÃO À LUZ DA TEORIA DE GRAMSCI
Authors: Toretta Zen Eliesér , Senra Avancini Bendineli Lisandra , Oliveira Luzinete de Souza 
Published: July 2023

Este trabalho tem como objetivo fazer uma reflexão acerca do Plano Nacional de Educação (PNE) 2014, em especial a “Meta 4”, aos pressupostos de escola integral de Antônio Gramsci. Ao longo da pesquisa observamos algumas incongruências na “Meta 4”, pois, se a proposta de universalização do ensino para aluno público alvo da educação especial, aproxima-se dos princípios desse teórico, no que se relaciona à proposta de escola de formação integral e para todos, contraditoriamente, o mesmo documento, configura-se como espaço de disputas de forças hegemônicas no qual determinados grupos apropriam-se das contradições apresentadas na lei, para ofertar um ensino segregador e implementar na sociedade ideias da classe dominante. Percebemos que embora os escritos deste autor perduram mais de um século os mesmos continuam atuais. Nota-se ainda, que o mesmo documento apresenta um caráter progressista e contraditório com relação ao AEE, configurando-se como espaço de luta pela hegemonia.
Balance of Private and Public Interest Law in Matters of Restricting Human Rights for the Purposes of National Security
Authors: Stetsenko Valentina , Havronska Tetiana , Makarova Olena , Konchakovska Valentyna , Nataliia Derkachova 
Published: July 2023

The work deals with the issues of determining the balance of private (individual) and public (state) interests in terms of admissibility of limiting human rights and freedoms in cases where it increases or prevents threats to national security. This was the aim of the study, as this issue has become highly relevant because of the growing number of hybrid threats to national security, which can be countered through the introduction of separate restrictions on human rights by the state. The systemic approach, the hermeneutic, and doctrinal approach were used to conclude that almost all constitutions, constitutional acts and constitutional laws of the EU and NATO member states provide for the possibility of restricting human rights. Such restrictions are imposed for the purposes of national security, public necessity or national interests of the state. Moreover, the list of cases where the restrictions on civil rights may be applied in the national security interests in national legislation is often much wider than in international legal acts. This demonstrates the primacy of national interests over individual interests, which is defined by national legislators as the common good at the constitutional level. The lack of detailed cases and models of state response to these processes determines the conclusions on the need to introduce a system of legally determined conditions for the application of restrictive measures. That is why further research should focus on identifying and detailing the criteria for applying measures to restrict civil rights for the purposes of national security at the constitutional level.

Received: 15 May 2023 / Accepted: 21 June 2023 / Published: 5 July 2023
Subjective Well-Being and Psychological Resilience as the Antecedents of Digital Entrepreneurship Intention
Authors: Sahrah Alimatus , Guritno Purnaning Dhyah , Rengganis Rani P. , Dewi Ros Patriani 
Published: July 2023

Digital entrepreneurship (DE) refers to the core scopes of the entrepreneur, entrepreneurial process, and ecosystem. Entrepreneur relates to digital behavior patterns, social impact, and knowledge, including the digital entrepreneurial intention of students to start a business. The current research aims to empirically examine personal characteristics covering psychological resilience, subjective well-being, demographic characteristics, and higher education service quality as environmental factors that might impact student’s digital entrepreneurship intention (DEI). A total of 305 responses were collected from undergraduate and graduate students in Yogyakarta, Indonesia, using a questionnaire consisting of four scales distributed online from various fields of study. The validity and reliability of the measurements and hypothesis testing were tested using SmartPLS. The research results show that service quality, student’s subjective well-being, and resilience positively influence their digital entrepreneurship intention. The moderating effect of the period of study on service quality and subjective well-being was rejected, while the moderating effect of gender was supported. However, the moderating effect of the period of study on student resilience was supported, and the moderating effect of gender was rejected. Therefore, universities and policymakers should focus on providing excellent services and support to improve students’ digital entrepreneurship intention and consider gender differences in designing relevant programs.

Received: 17 April 2023 / Accepted: 16 June 2023 / Published: 5 July 2023
Transfigurations of the Myth of Narcissus - Intertextuality, Re-Contextualization and Symbolism of Reflection: Oscar Wilde's "The Picture of Dorian Gray" and Ernest Koliqi's "Pasqyrat e Narcizit" (“The Mirrors of Narcissus”)
Authors: Buzhala Saranda , Berisha Labinot 
Published: July 2023

Narcissism has long been a significant topic in various fields, including art, literature, and the sciences. In contemporary times, its relevance has only grown due to the modern obsession with self and self-reflection. This paper explores the theme of narcissism as a literary motif in two works: Oscar Wilde's "The Picture of Dorian Gray" and Ernest Koliqi's "Pasqyrat e Narçizit" ("The Mirrors of Narcissus"). By examining the transfigurations of the narcissistic model, the study seeks to analyze the intertextual connections between the original myth and its recontextualized versions presented by these authors. The article aims to present the abstract interpretations of this myth that these authors have crafted in their literary works, employing dialectical approaches. The recontextualization of the narcissistic motif through literary aesthetics opens up opportunities for valuable comparisons, analyses, and interpretations of the symbol of reflection, the search for meaning, inter- and hypertextual relationships, as well as the exploration of pathological or social identity aspects associated with this figure.

Received: 21 April 2023 / Accepted: 16 June 2023 / Published: 5 July 2023
A Strategic View of a Fashion Design Programme: What Can We Do Better?
Authors: Mbatha Sipho 
Published: July 2023

This paper is based on a review of Walter Sisulu University documents and the researcher’s five-year lecturing experience in the WSU Department of Fashion’s Fashion Design Programme (FDP). The paper was inspired by the department’s inability to plan, implement, and oversee operational strategies to meet the FDP’s missions and vision statements, which negatively impacted the programme’s portability and cost. I found that academic staff were unable to further their qualifications or produce research despite being given eight hours per week to spend on research or qualification development. The WSU FDP also overspends the money in the students‘ materials accounts. The FDP has too many subjects, negatively affecting its graduation rates since 2008. I further found that the introduction of computer-aided design as a teaching and learning strategy would cut Creative Design II & III and Technical Drawing II & III expenditure by R175 022 compared to the 2015 costs. The FDP bought machinery in 2009 that was costly for the university and yet remains unused due to space and power limitations. The FDP could financially contribute more meaningfully to WSU’s stability and viability if the FDP were offered a spacious building and could develop and implement an extended programme. I conclude this paper by making recommendations for how WSU can improve the FDP, meet the FDP’s mission and vision statements, and strengthen its viability.

Received: 9 February 2023 / Accepted: 17 June 2023 / Published: 5 July 2023
The Impact of Modern Education Technologies on the Development of Vocational Qualification Skills in Light of the Requirements of the Labor Market
Authors: Alshuaibi Amani Hamad Mansour 
Published: July 2023

The study aims to know the role of modern education technologies in developing vocational qualification skills for diploma students at Umm Al-Qura University. This study used the quantitative approach to achieve the goal of the study, the questionnaire was used as a data collection instrument, and the study population included (777) students diploma of students studying the course of vocational qualification skills in the Applied College at Umm Al-Qura University and the study sample that was reached (534) Students, and the questionnaire was distributed electronically for the target sample, and the results of the study showed that diploma students have the average level in the use of modern educational technologies. The results also showed that modern education technologies have a role in developing vocational qualification skills for diploma students at Umm Al-Qura University.

Received: 4 February 2023 / Accepted: 20 May 2023 / Published: 5 July 2023
PENGARUH PENGETAHUAN ZAKAT, PENDAPATAN, KEPERCAYAAN, KUALITAS PELAYANAN TERHADAP MINAT APARATUR SIPIL NEGARA (ASN) MEMBAYAR ZAKAT PROFESI MELALUI BAZNAS KABUPATEN DEMAK
Authors: Fitriyani Lailatul , Irkhami Nafis 
Published: July 2023

The purpose of this study is to determine the influence of Zakat knowledge, income, trust, and service quality on the interest of State Civil Servants (ASN) to pay professional zakat through Baznas Demak Regency in 2021. The type of research used in this study is field research using a quantitative research approach. The data collection method was carried out through a questionnaire which was distributed to ASNs in the Demak Regency. Samples were taken as many as 100 with the purposive sampling technique. The data obtained then processed using the IMB SPSS 19 tool. This analysis includes the validity test, reliability test, normality test, heteroscedasticity test, multicollinearity test, multiple linear regression analysis, Ftest test, T-test test, and determinant coefficient test (R2). Based on the results of the T-test test, it shows that the variables of Zakat knowledge, income, and service quality have a positive and significant effect on the interest of ASN to pay professional Zakat through Baznas Demak Regency. On the other hand, the trust variable has a positive and insignificant effect on the interest of ASN to pay professional Zakat through Baznas in Demak Regency. The results of the F-test test show that the variables of Zakat knowledge, income, trust, and service quality together affect the interest of ASN to pay professional Zakat through Baznas in Demak Regency.