bluuebunny/crossref_metadata_2025_split huggingface dataset:
Increased incidence of congenital hypothyroidism in China: an analysis of 119 million screened newborns
Authors: Yao Yongna , Deng Kui , Zhu Jun , Xiang Liangcheng , Yuan Xuelian , MSE Qi Li , Liu Lei , GUO Yingkun , Liu Hanmin , Li Xiaohong 
Published: January 2023

Abstract
Purpose: The incidence of congenital hypothyroidism (CH) in China has increased though no nation-wide epidemiology has reported. To evaluate its trends and explore the possible reasons behind it.
Methods: Data of screened newborns from the Chinese Newborn Screening Information System from 2012 to 2019 was collected. We applied a Bayesian Hierarchical Poisson Regression model, meta-analysis, and several quantitative analyses to estimate incidence or proportion over years.
Results: 119,230,553 screened neonates and 56,617 CH cases were collected. The estimated CH incidence increased from 4.01 per 10,000 births in 2012 to 5.77 per 10,000 births in 2019. The average annul growth rate (ARG) of CH incidence for all provinces varied from 0.59% to 20.96%. Incidences of cases with initial TSH concentration of <10 mIU/L rose most rapidly. The results of meta-analysis showed the proportion of permanent CH increased by 0.024% (0.011%, 0.037%) per year. Each one-unit (mIU/L) decrease in TSH cutoffs value was associated with a 2.96% increase in CH incidence. The proportion of premature CH cases in the total number of CH increased from 6.60% to 9.10%, the increase of which was much higher than that of preterm birth rate in the same period. The provincial growth rate of screening coverage and provincial baseline CH incidences showed no significant association with their annual growth rates of CH incidence.
Conclusion: CH incidence has substantially increased in China. The slight adjustment of TSH cutoff value, and improvement of screening algorithm for preterm newborn might contribute to such a trend, however, their contribution is limited.
Combined transcriptomic and metabolomic analyses reveal the phosphorus stresses response mechanisms of Artemisia argyi
Authors: Wang Zixin , Ma Lin , Chen Changjie , Sun Haodi , Liu Anzheng , Guo Lujuan , Zhao Tingting , Liu Dahui 
Published: January 2023

Abstract
Purpose
Enzymatic activity in plant signaling, photosynthesis, energy metabolism, and respiratory activities depend on the amount of readily available phosphorus in the soil. Artemisia argyi(A. argyi) is a medicinal plant with antifungal, antiinsect, and anti-inflammatory activities. Our goal was to increase knowledge of the physiological and molecular mechanisms involved in the investigation of the effects of soil phosphorus on important secondary metabolites of A. argyi that regulate growth.
Methods
At various phosphorus levels, A. argyi was examined for its agronomic characteristics, elemental content in the leaves, generation of significant metabolic chemicals, and molecular mechanisms.
Results
A. argyi displayed the highest levels of phenolic acids (chlorogenic acid, isochlorogenic acid A, isochlorogenic acid B, isochlorogenic acid C, and neochlorogenic acid) and flavonoids (hispidulin, jaceosidin, eupatilin, and casticin), measured in the leaves, when treated with low levels of phosphorus. P-element levels significantly correlated with the amount of each active ingredient, and transcript Phosphorus levels also affected terpenoid content and the expression of numerous genes involved in the 4-hydroxytoluene pathway (MEP) and the pentadienyl pathway (MVA), which are both necessary for terpenoid production.
Conclusions
Low levels of phosphorus application enhance the accumulation of medicinal chemicals in A. argyi, medium levels of phosphorus application stimulate the growth of A. argyi, and associated genes are often down-regulated with higher levels of phosphorus application.
Phosphorylation state of S6K1 is redundant for its interaction with F actin
Authors: Latoo Shafat A. , Andrabi Khurshid I 
Published: January 2023

Abstract
Ribosomal protein S6 kinase 1(S6K1) is an evolutionary conserved kinase that is activated in response to growth factors and viral stimuli to influence cellular growth and proliferation. The serine/threonine kinase, S6K1 which is a downstream effector of phosphatidylinositol 3- kinase / Akt pathway, is frequently activated in certain types of cancers. S6K1 acts as an actin filament cross linking and as a Rho family of GTPase activating protein. We here present the evidence for domain specific interaction of S6 kinase 1 (S6K1) with filamentous actin or F actin. We show for the first time that [∆NH2-146 / ∆CT240 a. acid] region of S6K1 is actually responsible for its discrete binding to F actin. We also provide evidence that the binding of S6K1 to filamentous actin is phosphorylation independent and not facilitated by any other protein rather direct interaction and we couldn’t observe any interaction of S6K1 for monomeric actin (G actin) .By a time course experiment, we could found that the presence of S6K1 did not affect the kinetics of spontaneous actin polymerization but it enforces stability in F actin by cross linking it and rendering it more stable in the form of multifilament bundled actin. Using electron microscopy we found that these closely apposed bundles were often slightly curved, suggesting flexible cross linking. We further observe that S6 kinase 1 continued to exhibit sensitivity towards filamentous actin that remained unaffected by deletions compromised for [∆NH2-146 / ∆CT104] or [∆NH2-46] / ∆CT104] [∆NH2-146] or [∆NH2-46] or [∆CT104] . By computational study we found that [∆NH2-146 / ∆CT240 a. acid] region of S6K1 is rich in hydrophobic amino acids and has predominant α helical and coiled coil structure which serves as a structural basis for some of the actin binding proteins. These data together with the ability of the S6K1 to bind to F actin indicate that binding is phosphorylation independent, direct and facilitated by the [∆NH2-146 / ∆CT240 a. acid] region of S6K1.
Assessment of pulmonary artery size at birth as a prognostic factor in congenital diaphragmatic hernia: results of a multicenter study in Japan
Authors: Okazaki Tadaharu , Terui Keita , Nagata Kouji , Hayakawa Masahiro , Okuyama Hiroomi , Amari Shoichiro , Masumoto Kouji , Yamoto Masaya , Inamura Noburu , Toyoshima Katsuaki , Furukawa Taizo , Okawada Manabu , Yokoi Akiko , Koike Yuki , Nojiri Shuko , Nishizaki Yuji , Yanagisawa Naotake , Usui Noriaki 
Published: January 2023

Abstract
Objective: To assess the reliability of pulmonary artery (PA) parameters as a prognostic marker in neonates with isolated left-sided congenital diaphragmatic hernia (IL-CDH).
Study design: A retrospective cohort study conducted by the Japanese CDH Study Group (JCDHSG).
Results: 323 IL-CDH patients registered with the JCDHSG were included. 272 patients survived to 90 days of age. Right PA (RPA) and left PA (LPA) diameters and pulmonary artery index (PAIndex) at birth were significantly larger in survivors. The cutoff values of RPA and LPA diameters and PAIndex for survival up to 90 days were 3.2 mm, 2.8 mm and 83.7, respectively, and logistic regression analysis showed that these were significantly related to survival. Multiple logistic regression analysis showed that both the PA parameters and liver herniation were significantly related to survival.
Conclusions: Three PA parameters at birth predict clinical outcomes, and are considered as independent risk factors of liver herniation.
Preparation of core/shell Fe3O4@SiO2 .DIL as a magnetically heterogeneous catalyst for the synthesis of 1, 8‐dioxodecahydroacridine
Authors: Ebadi Amin , Vadie Sanaz 
Published: January 2023

AbstractIn this research design and synthesis of the magnetic nanoparticle, coating by a silica shell and modified with dicationic ionic liquid is described. Characterization of Fe3O4@SiO2.DIL was done by means of techniques such as XRD, FTIR, SEM, EDX, TEM, and TGA. The catalytic activity of Fe3O4@SiO2.DIL as a new nanocatalyst was tested in the the one-pot tree-component synthesis of 1,8-dioxodecahydroacridines. To achieve higher yield of products, factors such as reaction temperature, reaction time, the amount of nanocatalyst and solvent were studied, and optimized conditions were investigated. The results indicated that the reaction time for aromatic aldehydes bearing substituent at para position is shorter and higher yields of products were obtained with these substrates. Short reaction time, high yield of products, easy purification and easy recoverable catalyst without significantly less of its activity are the main advantages of the process presented. The results clearly show that the existing catalytic system could be eco-friendly and economical to carry out this reaction.
Exponential stability of Lord Shulman thermoelastic system with porous damping and distributed delay term
Authors: Bouzettouta Lamine , Boulkheloua Chaima , Khochemane Houssem Eddine , Karek Widad 
Published: January 2023

Abstract
In this paper, we consider a one-dimensional Lord-Shulman thermoelastic system 1 with porous damping and distributed delay term acting on the porous equation. Under suitable assumptions on the weight of distributed delay, we establish the well-posedness of the system by using semigroup theory and we show that the dissipations due to thermal effects with porous damping are strong enough to stabilise the system exponentially, independently of the wave speeds of the system.
A Prognostic Model Based on Nutritional Indexes for Patients with Pan-Carcinomas: A Real-World Cohort Study
Authors: Zheng Lin , Yu Qian-Qian , Ruan Wen-Bin , Chen Jin , Deng Qing-Hua , Zhang Ke , Jiang Xu-Li , Jiang Wen-Jun , Cai Dan-Na , He Chen-Jie , Wang Yu-Feng , Jiang Shen-Li , Chen Ming-Cong , You Guang-Xian , Ying Rong-Biao , Zhou Zhi-Rui 
Published: January 2023

Abstract
Background
Cancer patients have a high incidence of malnutrition, but traditional nutritional screening tools have low sensitivity and specificity, so they cannot properly stratify patient prognosis. Thus, we aimed to identify the nutritional indexes associated with patient prognosis, construct a prognostic model, and develop a nomogram for predicting individual survival probability.
Methods
Based on real-world data, patients admitted to the Department of Chemotherapy & Radiotherapy in Taizhou Cancer Hospital from January 1, 2017, to July 1, 2020, were included in the analysis. We collected nutritional indicators, clinicopathological characteristics, and previous major treatment details of the patients. The enrolled patients were randomly divided into training and validation cohorts in a 7:3 ratio. Lasso regression cross-validation was used in the training cohort to determine the variables to include in the Cox regression model. The training cohort was used to build the prediction model, and the validation cohort was used to further verify the discrimination, calibration and clinical effectiveness of the model.
Results
A total of 2,020 patients were included. The median follow-up time was 33.48 months (IQR, [15.79, 56.73] months), and the median OS was 56.50 months (95% CI, 50.36–62.65 months). In the training cohort of 1,425 patients, through Lasso regression cross-validation, thirteen characteristics were included in the model: sex, age, baseline weight, food intake reduction grade, emerging disease, ECOG performance status, hospitalization frequency, prealbumin, albumin, clinical stage, hemoglobin suppression grade, platelet suppression grade, and liver function classification. Based on these factors, a Cox proportional hazards model was developed and visualized as a nomogram. The C-indexes of the model for predicting 1-, 3-, 5- and 10-year OS were 0.848, 0.826, 0.814 and 0.799 in the training cohort and 0.851, 0.819, 0.814, and 0.801 in the validation cohort. The model showed great calibration in the two cohorts. Patients with a score of less than 274.29 had a better prognosis (training cohort: HR, 6.932; 95% CI, 5.723–8.397; log-rank P < 0.001; validation cohort: HR, 8.429; 95% CI, 6.180-11.497; log-rank P < 0.001).
Conclusions
The prognostic model based on the nutritional indexes of patients with pan-carcinomas can divide patients into different survival risk groups and performed well in internal validation.
Human exposure to diesel exhaust induces CYP1A1 expression via AhR activation without a coordinated antioxidant response
Authors: Friberg Maria , Behndig Annelie Flodström , Bosson Jenny Ann , Muala Ala , Barath Stefan , Dove Rosamund , Glencross Drew , Kelly Frank James , Blomberg Anders , Mudway Ian Stanley , Sandström Thomas , Pourazar Jamshid 
Published: January 2023

Abstract
Background
Diesel exhaust (DE) induces neutrophilia and lymphocytosis in experimentally exposed humans. These responses occur in parallel to nuclear migration of NF-κB and c-Jun, activation of mitogen activated protein kinases and increased production of inflammatory mediators. There remains uncertainty regarding the impact of DE on endogenous antioxidant and xenobiotic defences, mediated by nuclear factor (erythroid-derived 2)-like 2 (Nrf2) and the aryl hydrocarbon receptor (AhR) respectively, and the extent to which cellular antioxidant adaptations protect against the adverse effects of DE.
Methods
Using immunohistochemistry we investigated the nuclear localization of Nrf2 and AhR in the epithelium of endobronchial mucosal biopsies from healthy subjects six-hours post exposure to DE (PM10, 300 µg/m3), as a marker of activation. Cytoplasmic expression of cytochrome P450s, family 1, subfamily A, polypeptide 1 (Cyp1A1) and subfamily B, Polypeptide 1 (Cyp1B1) were examined to confirm AhR activation; with the expression of aldo-keto reductases (AKR1A1, AKR1C1 and AKR1C3), epoxide hydrolase and NAD(P)H dehydrogenase quinone 1 (NQO1) also quantified. Inflammatory and oxidative stress markers were examined to contextualize the responses observed.
Results
DE exposure caused an influx of neutrophils to the bronchial airway surface (p = 0.013), as well as increased bronchial submucosal neutrophil (p < 0.001), lymphocyte (p = 0.007) and mast cell (p = 0.002) numbers. In addition, DE exposure enhanced the nuclear translocation of the AhR and increased the CYP1A1 expression in the bronchial epithelium (p = 0.001 and p = 0.028, respectively). Nuclear translocation of AhR was also increased in the submucosal leukocytes (p < 0.001). Epithelial nuclear AhR expression was negatively associated with bronchial submucosal CD3 numbers post DE (r=-0.706, p = 0.002). In contrast, DE did not increase nuclear translocation of Nrf2 and was associated with decreased NQO1 in bronchial epithelial cells (p = 0.02), without affecting CYP1B1, aldo-keto reductases, or epoxide hydrolase protein expression.
Conclusion
These in vivo human data confirm earlier cell and animal-based observations of the induction of the AhR and CYP1A1 by diesel exhaust. The induction of phase I xenobiotic response occurred in the absence of the induction of antioxidant or phase II xenobiotic defences. Thus, DE-associated compounds, such as polycyclic aromatic hydrocarbons (PAHs), induce detoxification pathway and acute inflammation without concomitant protective cellular adaptations.
Cancer Screening Behaviors and Health Literacy Levels of Women aged 30 and over
Authors: Çelikkalp Ülfiye , Irmak Aylin Yalçın 
Published: January 2023

AbstractPurpose This study aimed to determine the cancer screening behaviors of women over 30 and the factors affecting them during the Covid-19 pandemic. Methods This cross-sectional study was conducted with 301 women aged 30 and over in Edirne, the border province in northwest Turkey. Study data were collected with the Personal Information Form and Turkiye Health Literacy Scale-32 (THL-32). Results The mean age of the participants was 43.66 ± 9.02; 65.1% were between the ages of 30–49, and 85% were married. 74.8% of the participants did not have a screening test during the pandemic. Women underwent breast examination (32.2%), pap smear test (21.9%), and fecal occult blood test (19.9%), respectively, with an attending physician. The mean health literacy score of the participants was 28.18 ± 7.55, and the majority (80.7%) were in the category of inadequate-limited health literacy. The mean health literacy score and breast cancer screening were statistically significant (p < 0.05). Conclusion The study group's participation status and health literacy levels in cancer screening tests were low. The COVID-19 pandemic period negatively affected the individuals' participation in screening tests.
Tailored versus conventional surgical debridement in complex facial lacerations in emergency department: a retrospective study
Authors: Park Byeong Kwon , Min Jin Hong , Park Jung Soo , You Yeon Ho , Jeong Won Joon , Cho Yong Chul , Oh Se Kwang , In Yong Nam , Ahn Hong Joon , Kang Chang Shin , Kyung Hyun woo , Kim Joo Hak , Yang Ho Jik , Lee Byung Kook , Yoo Heon Jong 
Published: January 2023

Abstract
Background
Surgical debridement is usually an essential step in treating complex facial lacerations (CFL). As the severity of CFL increases, conventional surgical debridement (CSD) of wound edges becomes difficult and may be insufficient to remove damaged tissues and create clean edges. Because the severity and shape of CFL are diverse and different, it is necessary to tailor the customized pre-excisional design, the tailored surgical debridement (TSD), for each case before performing surgical debridement. Applying TSD is expected to enable effective debridement in CFL with higher severity. This study compared cosmetic outcomes and complication incidence of CSD and TSD, according to CFL severity.
Methods
This was a retrospective observational study. Eligible patients with CFL who visited the emergency department between August 2020 and December 2021 were examined. CFL severity was graded to Grades I and II. CSD and TSD outcomes were compared using the Scar Cosmesis Assessment and Rating (SCAR) scale. Furthermore, a good cosmetic outcome was defined as a SCAR score of ≤ 2. The percentage of good cosmetic outcome between the two groups was compared. The SCAR score and percentage of good cosmetic outcomes between the two groups were compared overall and by severity. For comparison of complication incidence, asymmetry, infection, and dehiscence incidence were compared.
Results
In total, 252 patients were enrolled (121 [48.0%] CSD and 131 [52.0%] TSD). In CSD and TSD groups, the median SCAR scores were 3 (1–5) and 1 (0–2) in all enrolled patients (p < 0.001), 2 (0–4) and 1 (0–1) in Grade I (p < 0.01), and 5 (4–6) and 1 (1–2) in Grade II (p < 0.001). The good cosmetic outcomes were 46.3% and 84.0% overall (p < 0.001), 59.6% and 85.0% in Grade I (p < 0.01), and 9.4% and 83.5% in Grade II (p < 0.001) for CSD and TSD. The incidence of complications was significantly higher in the CSD than in the TSD groups, but this was limited to asymmetry. There was no statistical difference in both infection and dehiscence.
Conclusion
TSD can lead to an objectively good cosmetic prognosis at higher CFL severity than CSD and can reduce the occurrence of facial asymmetry.
Genetic analysis of brown planthopper, Nilaparvata lugens (Stål.) (Hemiptera: Delphacidae) based on DNA markers
Authors: Babu Soumya Bharati , Govindharaj Guru-Pirasanna-Pandi , C Parameswaran , Padhi Jayaraj , G Basana-Gowda , M Annamalai , Patil Naveenkumar , Meher Chanchala , S Sabarinathan , Rath Prakash Chandra 
Published: January 2023

Abstract
Brown planthopper, Nilaparvata lugens (Stål.) is one of the most ruinous pest of rice in south-east Asia. This insect had the tendency to express differential reaction to resistant rice cultivars and various insecticide groups in different geographic locations. Therefore, genetic diversity among the N. lugens populations is required to be understood for their effective management. Hence, in the present study, genetic structure and genetic diversity of 22 N. lugens populations collected from 22 hotspot regions of India were studied with genomic simple sequence repeats (NlSSR) markers. Results revealed that mean genetic diversity was 0.399 and polymorphic information content (PIC) was 0.337 in the 30 selected SSR markers. Further, molecular variance (AMOVA) revealed only 2% variation among population and 98% within the populations. In cluster and population structure analysis, all the 22 population were sub-grouped into three groups. Interestingly, north and west Indian population showed high genetic similarity and assembled into one cluster in cluster analysis. The east and south Indian populations were evenly segregated into rest two clusters. Similarly, north and west Indian population again deemed to be occupied same compartment in Principal coordinate analysis (PCoA). This variation might be associated with the N. lugens migration due to wind movement of south-west monsoon in two branches viz. Arabian sea branch and Bay of Bengal branch. Present study provides the molecular evidence of population genetics of N. lugens in India and the information could be useful to devise the efficient management strategy against this pest in different rice ecosystem.
Dental injuries in the pediatric emergency department: A retrospective analysis and literature review
Authors: Cohen Neta , -Kagan Renana Via , Haberman Shira , Gigi Dana , Ianculovici Clariel , Rimon Ayelet 
Published: January 2023

Abstract
This retrospective cohort study included all patients aged 0-18 years who presented to our pediatric emergency department (PED) between 2017 and 2021 following injuries of primary and permanent teeth. We explored risk factors for the need for dental intervention that required urgent consultation with an oral and maxillofacial specialist and included: tooth repositioning, splinting, extraction, replantation, intraoral soft tissue laceration repair, and surgical intervention. Of the total of 830 patients (mean age 7.1±3.9 years, 589 males [71.0%]), 237 (28.5%) required dental intervention. All patients with alveolar fractures and those with involvement of permanent teeth with extrusive luxation mandated urgent dental consultation. Additional independent predictors for dental intervention for primary tooth injury were: root fracture (aOR 38.4 [95%CI: 3.95–373.22, p = 0.002), facial bone involvement (aOR 12.40 [95%CI: 2.33–65.93], p = 0.003), lateral luxation (aOR 6.9 [95%CI: 4.27–11.27], p < 0.001), extrusive luxation (aOR 6.44 [95%CI: 2.74-15.14, p < 0.001), and avulsion (aOR 2.06 [95%CI: 1.23–3.45], p= 0.006). Additional independent predictors for permanent tooth injury were: lateral luxation (aOR 27.8 [95%CI: 6.1–126.6], p < 0.001) and avulsion (aOR 6.8 [95%CI: 2.9-15.9], p < 0.001).
Conclusion: Alveolar fracture is the most severe pediatric dental injury, requiring immediate dental intervention for injuries of both primary and permanent teeth. Tooth luxation with significant mobility or malocclusion, incomplete avulsion, a suspected root involvement, or facial bone injury in the primary teeth, and tooth luxation (extrusive/lateral) and avulsion in the permanent teeth dictate urgent dental consultation and intervention. Clinical algorithms for dental injuries management are suggested.