bluuebunny/crossref_metadata_2025_split huggingface dataset:
Occupational exposures and coronary heart disease in the Hamburg City Health Study (HCHS) – a cross-sectional study
Authors: Labe Franziska , Twerenbold Raphael , Toprak Betül , Koch Peter , Zyriax Birgit-Christiane , Affolderbach Sarah , Damerau Lukas , Hoven Hanno , Zeeb Hajo , Herold Robert , Harth Volker 
Published: January 2025

Abstract

Background
Coronary heart disease (CHD) is the leading cause of death among adults in Germany. There is evidence that occupational exposure to particulate matter, noise, psychosocial stressors, shift work and high physical workload are associated with CHD. The aim of this study is to identify occupations that are associated with CHD and to elaborate on occupational exposures associated with CHD by using the job exposure matrix (JEM) BAuA-JEM ETB 2018 in a German study population.

Methods
Cross-sectional data from 8,070 participants, members of the first sub-cohort of the Hamburg City Health Study (HCHS), was used. To classify occupations, we rely on standard occupational titles (ISCO-08). The level of exposure is assigned to each job using a JEM. CHD is measured by self-reported diagnosis. Absolute and relative frequencies were calculated. Using logistic regression, the association of CHD and standard occupation titles via ISCO-08 and the association of CHD and occupational exposures via JEM were calculated and adjusted for potentially confounding covariates. Multiple imputations with chained equations (MICEs) were applied for missing values. Sensitivity analyses were performed.

Results
The CHD prevalence in the study population was 4.6% (95% CI 4.2–5.1). Occupations associated with CHD were Physical and Engineering Science Technicians, Other Health Associate Professionals, General Office Clerks, Secretaries (general), Material Recording and Transport Clerks, Hairdressers, Beauticians and Related Workers, Electronics and Telecommunications Installers and Repairers, Other Craft and Related Workers, Car, Van and Motorcycle Drivers, Mobile Plant Operators and Domestic, Hotel and Office Cleaners and Helpers. Among occupational exposures retrieved from the JEM, Environmental Demands showed an association with CHD in the crude model but not after adjustment. The results remained robust in sensitivity analyses.

Conclusions
This study is the first to assess the association of a wide range of occupations and occupational exposures with CHD in a German study population. We found no association between occupational exposures and CHD after adjustment, but 11 occupations associated with CHD were identified. The results are limited by cross-sectional design, healthy worker effect (HWE), and small group sizes. Further studies with a larger sample and longitudinal design containing data on occupational history, occupational exposures and time of CHD diagnosis are needed.
Use of virtual reality to remotely train healthcare professionals in paediatric emergency tracheostomy skills: protocol for a multi-centre, non-inferiority educational interventional study with historical controls
Authors: Abbas Jonathan R. , Younis Noorulanne , Johnstone Emily , Rajai Azita , Isba Rachel , Payton Antony , McGrath Brendan A. , Tolley Neil , Bruce Iain A. 
Published: January 2025

Abstract

Background
The insertion of a tracheostomy is an established technique used to wean patients off ventilatory support, manage secretions in complex conditions, and as a potentially life-saving procedure to bypass upper airway obstruction. Life-threatening complications during aftercare are not uncommon and may be influenced by a lack of education of carers or healthcare providers of children and young people living with a tracheostomy. Education programmes designed and supported by the National Tracheostomy Safety Project are effective, but resources are not available to educate the workforce at scale. With the overarching aim of widening access to paediatric tracheostomy skills training, we present the protocol for the development and evaluation of a novel virtual reality (VR) training tool designed to simulate the emergency management of paediatric tracheostomy complications.

Methods and discussion
A multi-centre, non-inferiority educational interventional study with historical controls will be used to evaluate the novel VR training package. A group of 69 healthcare staff and students will have one week to use the educational intervention as often as necessary to learn paediatric emergency tracheostomy skills. The primary outcome measure is skill performance in simulation in a pre- and post-intervention structure within subjects. Participant performance will also be assessed using non-inferiority metrics against historical traditional educational control data. Secondary outcomes include knowledge gain, knowledge retention, usability, side effects, and participant satisfaction. To minimise the risk of cybersickness, teleportation was the preferred locomotion method for the user navigation within the VR environment.

Trial registration
Full registration of this study was completed at ClinicalTrials.gov. The registration number is NCT06350708 and was accepted on the 4th April 2024.
22 months follow-up of deep marginal acquisition with thermacut bur in management of deep subgingival interproximal carious lesions: a case report
Authors: Elmorsy Ahmed Khairy , Ibrahim Shereen Hafez , El-Nahass Hani Mohamed Essam , Zohairy Ahmed El 
Published: January 2025

Abstract

Background
Minimally invasive dentistry is now becoming the forefront of restorative dentistry, involving less traumatic treatment protocols, conservation of tooth structure and surrounding tissues, enhancing the long-term survivability of treated teeth, and improving the overall quality of life for patients.

Objective
The current case report was conducted to evaluate acquiring deep subgingival interproximal carious lesions by the mean of thermacut bur gingivectomy, in terms of patient satisfaction through pain evaluation, Bleeding on Probing, Pocket Depth, Crestal Bone Level evaluation, and restoration evaluation using modified USPHS criteria.

Material and methods
A patient with a deep proximal cavity in the posterior tooth was thoroughly examined and underwent Thermacut Bur Gingivectomy (TBG) after caries removal followed by direct resin composite restoration of the prepared cavity. Patient Satisfaction using a Visual Analogue Scale (VAS) as a primary outcome. Bleeding on Probing (BoP), Probing Depth (PD), and Crestal Bone Level (CBL) as secondary outcomes, and Marginal Integrity using Modified USPHS Criteria as a tertiary outcome, were evaluated at the baseline, immediate post-operative, 6 month, 12 month and 22 month follow-up intervals.

Results
Thermacut bur gingivectomy revealed minimal immediate post-operative pain, minimal time-consuming procedure, minimal (BoP), appropriate (PD) and no need for extra specialty involvement in the treatment of deep interproximal carious lesions in addition to preservation of the alveolar bone crest with excellent restoration margin at different time intervals.

Conclusions
Thermacut bur gingivectomy can be considered a valid treatment for managing of deep subgingival interproximal carious lesions in vital teeth. Thermacut bur gingivectomy can be introduced as an easy technique for clinicians in the management of deep subgingival interproximal carious lesions, without the need to refer patients to periodontists and without the need for special devices.
Effects of tongue tamers and customized bonded spurs as an early treatment of anterior open bite: a randomized clinical study
Authors: Alawy Safa B. , EL-Desouky Shaimaa S. , Kabbash Ibrahim A. , Hadwa Shimaa M. 
Published: January 2025

Abstract

Background
Anterior open bite is a challenging condition for pediatric dentists and orthodontists as it causes aesthetic, speech, feeding, and psychological problems; this emphasizes the need for early diagnosis and interception of this malocclusion.

Aim
This study aimed to evaluate the effects of prefabricated metal-bonded tongue tamers and customized bonded spurs in the early treatment of anterior open bite.

Materials and methods
A sample of seventy-five children aged 7–9 years were assigned into three groups in which anterior open bite was treated using tongue tamers (group-I), customized composite bonded spurs(group-II), and conventional fixed palatal cribs (group-III). Study model and cephalometric x-ray evaluations were done before and after a three-month follow-up. Data was gathered and statistically analyzed using ANOVA and Bonferroni tests.

Results
Model overbite at central and lateral incisors was increased in all groups. The highest increase was found in group-I(3.16 ± 1.17, 2.81 ± 0.94)and group-III(3.08 ± 1.10,2.99 ± 1.00) though the difference was not statistically significant. Also, cephalometric overbite was non-significantly increased in all groups with a high increase in group-III(3.13 ± 1.10). Overjet decreased in all groups, with the greatest reduction observed in group-I(-2.58 ± 1.02 and -2.47 ± 0.80 in model and cephalometric respectively) and was significantly different from group-II using pairwise analysis. There was a decrease in SNA and ANB in all groups with more significant improvement in groups-I(-1.20 ± 0.88,-1.65 ± 0.74) and -III(-1.31 ± 0.92, -1.62 ± 0.75) than group-II(-0.63 ± 0.46, -0.90 ± 0.43). Moreover, FMA measurements decreased significantly in group-I (-2.6 ± 1.11). Regarding SNB measurement, there was a non-significant increase in all three groups with the highest increase in group-I(0.49 ± 0.48) followed by group-III(0.34 ± 0.63). The U1/FHP and L1/GoGn angles were non-significantly decreased in all tested groups with the highest decrease in group-I(-1.76 ± 1.00 and-2.54 ± 0.87 respectively).

Conclusion
Early treatment of anterior open bite, along with tongue tamers' simplicity and aesthetics promoted the malocclusion correction and occlusal function restoration.

Trial registration
ClinicalTrials.gov, NCT05792553, “Effects of Tongue Tamers as an Early Treatment of Anterior Open Bite”, Retrospectively registered: 31/03/2023.
Immune responses of cattle vaccinated by various routes with Mycobacterium bovis Bacillus Calmette-Guérin (BCG)
Authors: Palmer Mitchell V. , Hwang Soyoun , Kanipe Carly , Putz Ellie J. , Fernandes Luis Guilherme Virgilio , Didkowska Anna , Boggiatto Paola M. 
Published: January 2025

Abstract

Background

Mycobacterium bovis BCG is the human tuberculosis vaccine and is the oldest vaccine still in use today with over 4 billion people vaccinated since 1921. The BCG vaccine has also been investigated experimentally in cattle and wildlife by various routes including oral and parenteral. Thus far, oral vaccination studies of cattle have involved liquid BCG or liquid BCG incorporated into a lipid matrix. Lyophilization is an established technique used for stabilizing bioproducts such as vaccines.

Methods
In the current study, cattle were vaccinated in two phases. In each phase, cattle were divided into three groups. Group 1 received BCG injected SQ, Group 2 received liquid BCG delivered to the posterior oral cavity, Group 3 orally consumed lyophilized BCG contained within a gelatin capsule placed within a small amount of a commercial alfalfa product.

Results
No vaccinated cattle were positive by an interferon gamma release assay. All but 4 animals were negative by tuberculin skin testing prior to vaccination: the 4 non-negative animals being categorized as suspects. Sixteen weeks post-vaccination all but 1 animal was negative, it being categorized as a suspect. An in vitro antigen stimulation assay and flow cytometry were used to detect antigen-specific CD4, CD8 and γδ T cell responses following vaccination. Oral vaccination of animals with lyophilized BCG did not result in any increases in the frequency of CD4, CD8 or γδ T cell proliferative or IFN-γ responses at any of the time points analyzed in either phase 1 or 2. In contrast, vaccination with BCG SQ and liquid BCG delivered to the posterior pharynx, resulted in an increase in the frequency of proliferating and IFN-γ-producing CD4 T cells with peak responses at 9–12 weeks post-vaccination. Similar to oral lyophilized BCG vaccinated animals, we did not observe any significant increases in the frequency of CD8 and γδ T cell proliferative and IFN-γ responses following SQ or oral liquid vaccinated animals.

Conclusions
These data would suggest that vaccination with oral lyophilized BCG does not induce a measurable, antigen-specific cell mediated responses in the periphery, when compared to BCG administered SQ or liquid BCG administered via the oral route. However, vaccination with either SQ or liquid BCG delivered to the posterior pharynx does induce measurable CD4 T cell responses in the periphery.
Multi-omics analysis in inclusion body myositis identifies mir-16 responsible for HLA overexpression
Authors: Wijnbergen Daphne , Johari Mridul , Ozisik Ozan , ‘t Hoen Peter A.C. , Ehrhart Friederike , Baudot Anaïs , Evelo Chris T. , Udd Bjarne , Roos Marco , Mina Eleni 
Published: January 2025

Abstract

Background
Inclusion Body Myositis is an acquired muscle disease. Its pathogenesis is unclear due to the co-existence of inflammation, muscle degeneration and mitochondrial dysfunction. We aimed to provide a more advanced understanding of the disease by combining multi-omics analysis with prior knowledge. We applied molecular subnetwork identification to find highly interconnected subnetworks with a high degree of change in Inclusion Body Myositis. These could be used as hypotheses for potential pathomechanisms and biomarkers that are implicated in this disease.

Results
Our multi-omics analysis resulted in five subnetworks that exhibit changes in multiple omics layers. These subnetworks are related to antigen processing and presentation, chemokine-mediated signaling, immune response-signal transduction, rRNA processing, and mRNA splicing. An interesting finding is that the antigen processing and presentation subnetwork links the underexpressed miR-16-5p to overexpressed HLA genes by negative expression correlation. In addition, the rRNA processing subnetwork contains the RPS18 gene, which is not differentially expressed, but has significant variant association. The RPS18 gene could potentially play a role in the underexpression of the genes involved in 18 S ribosomal RNA processing, which it is highly connected to.

Conclusions
Our analysis highlights the importance of interrogating multiple omics to enhance knowledge discovery in rare diseases. We report five subnetworks that can provide additional insights into the molecular pathogenesis of Inclusion Body Myositis. Our analytical workflow can be reused as a method to study disease mechanisms involved in other diseases when multiple omics datasets are available.
Low dose DNA methyltransferase inhibitors potentiate PARP inhibitors in homologous recombination repair deficient tumors
Authors: Pacaud Romain , Thomas Scott , Chaudhuri Sibapriya , Lazar Ann , Timmerman Luika A. , Munster Pamela N. 
Published: January 2025

Abstract

Background
Poly (ADP-Ribose) polymerase inhibitors are approved for treatment of tumors with BRCA1/2 and other homologous recombination repair (HRR) mutations. However, clinical responses are often not durable and treatment may be detrimental in advanced cancer due to excessive toxicities. Thus we are seeking alternative therapeutics to enhance PARP-directed outcomes. In an effort to expand the clinical use of PARP inhibitors to HRR proficient tumors, several groups have tested combinations of DNA methyltransferase inhibitors and PARP inhibitors. While this approach attenuated tumor cell proliferation in preclinical studies, subsequent clinical trials revealed little benefit. We hypothesized that benefit for this drug combination would only be specific to HRR deficient tumors, due to their inability to enact high fidelity DNA repair with subsequent cell death.

Methods
We generated hypomorphic BRCA1 and BRCA2 variants of the HRR proficient triple negative breast cancer cell line MDA-MB-231. We compared therapeutic response features such as RAD51 focus formation, cell cycle fraction alterations, DNA damage accumulation, colony formation, and cell death of these and other cell lines with and without intrinsic BRCA1/2 mutations. Results were confirmed in BRCA1/2 intact and deficient xenografts and PDX.

Results
Our targeted variants and cells with intrinsic BRCA1/2 mutations responded to low dose combination therapeutic treatment by G2M stalling, compounded DNA damage, severely attenuated colony formation, and importantly, increased cell death. In contrast, the parental MDA-MB-231 cells and other HRR proficient cell lines produced smaller cell populations with short term treatment, but with much less cumulative DNA damage, and minimal cell death. In animal studies, our BRCA-engineered hypomorphs and several independent PDX models with clinically relevant BRCA mutations were acutely more vulnerable to this drug combination.

Conclusions
We conclude that low dose DNA methyltransferase inhibition can cooperate with low dose PARP inhibition to increase DNA damage predominantly in cells with HRR deficiencies, ultimately producing more cell death than in HRR proficient tumors. We predict that clinical benefit will more likely be apparent in patients with DNA repair defective tumors and are focusing clinical exploration of this drug combination in these patients, with the goals of enhancing tumor cell death at minimal toxicities.
Adsorption characteristics and mechanism of Cd by mealworm frass
Authors: Kim Hae-Been , Lee Jae-Hoon , Lee Ye-Ji , Rho Jun-Suk , Lee Jeong-Min , Kim Seong-Heon , Park Jong-Hwan , Seo Dong-Cheol 
Published: January 2025

Abstract
This experiment was conducted to evaluate the adsorption–desorption characteristics and mechanisms of heavy metals by the mealworm frass (MF). The adsorption characteristics of Cd by MF were predominantly influenced by initial pH, MF dosage, temperature, and reaction time. The maximum adsorption capacity of Cd by MF was 48.1 mg/g, which was well described by Langmuir isotherm and pseudo-second-order models. The optimal desorption solution for separating Cd from Cd-adsorbed MF was 0.02 M HCl, which showed a high desorption efficiency of over 90%. In particular, the adsorption mechanism of Cd by MF was confirmed through functional group change, cation exchange, precipitation experiment, and it was found that Cd was predominantly affected by cation exchange and precipitation on the MF surface. The amounts of Cd fractionated by F1 (exchangeable) and F2 (bonded to carbonate) solutions were 58.9 and 25.2% of the total fractionated Cd amount, which means that Cd adsorbed by MF can be easily eluted/mobilized by environmental changes. Considering the above results, it is believed that MF can be used as an effective adsorbent to remove Cd. However, since the adsorption of Cd by MF is sensitive to environmental changes and the bond itself is weak, it is considered that a special management plan is needed.
Leveraging opportunities for self-regulated learning in smart learning environments
Authors: Adler Idit , Warren Scott , Norris Cathleen , Soloway Elliot 
Published: January 2025

Abstract
Smart learning environments provide students with opportunities to engage in self-regulated learning (SRL). However, little research has examined how teachers leverage these opportunities. We employed a multiple-case study methodology to examine the SRL supporting instructional practices of five third-grade teachers as they implemented a science unit embedded within a smart learning environment which enabled student engagement in SRL processes. The identified set of instructional practices was used to formulate the teachers’ SRL support profiles, which indicates the extent to which teachers provided students with opportunities to regulate the cognitive aspect of SRL. Our findings indicated that teachers’ interactions with the smart learning environment varied from teacher-focused use, restricting opportunities for SRL engagement, to student-focused use, leveraging opportunities for student engagement in SRL. These results demonstrate that, while smart learning environments potentially provide rich contexts for students’ engagement in SRL, how teachers use this environment through their SRL-supporting instructional practices determines students’ actual engagement in SRL.
Safe havens for Bitcoin and Ethereum: evidence from high-frequency data
Authors: Ali Fahad , Khurram Muhammad Usman , Sensoy Ahmet 
Published: January 2025

Abstract
Investing in cryptocurrencies is progressively becoming a norm; however, these assets are excessively volatile and often decrease or increase in value instantly. Thus, rational investors holding cryptocurrencies for extended periods firmly search for assets that can diversify their risk, preferably with assets other than cryptocurrencies. In this study, we consider the two most studied cryptocurrencies with the highest capitalization and trading volume/value, namely Bitcoin and Ethereum. Specifically, we examine whether high-performing leading US tech stocks (Facebook, Amazon, Apple, Netflix, Google [FAANG]) can provide any diversification benefits to cryptocurrency investors. To do so, we employ dynamic conditional correlation (DCC), asymmetric DCC, time-varying parameter vector autoregression-based connectedness measures, dynamic correlation-based hedge and safe-haven regression analyses, portfolio optimization and hedging strategies, time- and frequency-based wavelet coherence, and high-frequency 10-min intraday data from January 1, 2018 to January 31, 2023. We find that FAANG stocks can be considered (at least weak) safe havens for Bitcoin and Ethereum during the sample period. Our subperiod analyses reveal that the safe-haven role of FAANG stocks, specifically for Bitcoin, has noticeably increased. While the safe-haven property of Facebook is the most promising, for Netflix it is blurred between a weak–safe-haven and a hedge. Our findings may help investors, policymakers, and academicians to invest in cryptocurrencies, formulate relevant investment guidelines, and extend the literature on cryptocurrencies, respectively.