bluuebunny/crossref_metadata_2025_split huggingface dataset:
RELATIONSHIP BETWEEN RHEOLOGICAL PROPERTIES OF HYDROGELS, BIOPRINTING PARAMETERS AND 3D SCANNER TECHNOLOGY
Authors: Rodríguez Rego J M , Mendoza Cerezo L , Macías García A , Carrasco Amador J P , Marcos Romero A C , Díaz Parralejo A 
Published: January 2023

Abstract

Introduction
In bioprinting techniques, tissue engineering from 3D models to generate the desired geometry is as important as cell proliferation, as it will be key to the survival of the printed tissue and its functional success.

Methods
Alginate-based hydrogels were used because they are the most widely used by the scientific community for bioprinting.
The alginate-based hydrogels used were characterised using a rheometer to obtain the boundary conditions to be applied in the computational software.
A 2D axisymmetric model together with a two-phase flow level set interface of a bioprinting nozzle and a computational model of a human tooth were carried out to verify the results obtained.

Results
The data showed that droplet detachment occurs at lower pressures based on the higher the percentage of alginate. In addition, samples with a higher percentage of alginate were less sensitive to pressure during extrusion.

Conclusions
CONTENT VALIDATION OF THREE MODULES FOR ONLINE TRAINING OF MINIMALLY INVASIVE SURGERY
Authors: Sánchez-Peralta L F , Ortega-Morán J F , Sánchez-González P , Oropesa I , Juhos K , Tiu C , Pagador J B , Sánchez-Margallo F M 
Published: January 2023

Abstract

Introduction
Training tools should follow a thorough validation process that assure their value before their wider use. In this regard, three online modules for laparoscopic cholecystectomy, lumbar punction and knee arthroscopy have been created for an integrative Learning Management System (LMS) that connects external assets, therefore allowing training of technical and nontechnical skills. This work focuses on the content validation of the modules to determine the appropriateness of the material for learning purposes.

Methods
The contents for the three modules have been developed following a 3D pedagogical model designed for e-learning in minimally invasive surgery. Experts were requested to rate 10 items about the contents and the activities included within the modules. Rating was done on a 5-point Likert scale (5-Strongly agree, 1-Strongly disagree).

Results
Six experts took part in the study. Contents were well rated as unique (4.17), sufficient to cover the learning objectives (4.50), up to date (4.33), realistic and relevant (4.83), professional (4.67), with adequate assessment (4), useful forum (4.33), good quality multimedia contents (4) but not so innovative (3.83). Lastly, external assets provided added value to the courses (4.5).

Conclusions
In general, experts were satisfied with the structure and the quality of the education material and found them to be well prepared according to the best practice of each profession. Therefore, the modules will be incorporated in the LMS for training, which will be further validated to prove its utility as a training tool.
EXVIVO AND IN VIVO PORCINE ANIMAL MODEL FOR VALIDATION OF UNIVEC TRANSANAL DEVICE
Authors: Gómez Dovigo A , Noguera Aguilar J F , Aguirrezabalaga González J , Centeno Cortés A 
Published: January 2023

Abstract

Introduction
Colorectal cancer screening has led to a greater number of early lesions being diagnosed. At the rectal level, minimally invasive surgery has become more important since major surgery increases morbimortality. This experimental study evaluates the feasibility, safety, and effectiveness of the UNIVEC® device for both abdominal and transanal procedures and compares it to other devices prior to its commercialization and use in the clinical setting.

Methods
UNIVEC® is a transanal device with a pneumatically sealed channel that allows the introduction of the flexible endoscope and 2 accessory channels. Thirty-two porcine models were used in the 2-phase study.

Results
Phase I: 18 models and 2 procedures (hysterectomy and cystectomy) were used and UNIVEC® was compared with 2 devices (SILS®, GelPOINT®). Phase II: 14 models and 3 procedures (polypectomy, EMR, DSE). The final prototype design was satisfactory and met the established minimum performance requirements.

Conclusions
UNIVEC® is a transanal device that allows for hybridization of laparoscopic and endoscopic instrumentation. A model anatomically similar to that of the human was needed to assess intraoperative complications arising during surgery before its clinical application. The porcine model, due to its anatomical characteristics and experience, was the model of choice in our project. The ex vivo model facilitates learning the technique of the device prior to its use in vivo, which offers greater final cost-effectiveness.
LIVER REGENERATION AND CHEMOTHERAPY, IS IT FEASIBLE?
Authors: Novo Sukia I , Mendia Bravo M , Gil Rodríguez E , García-Alonso I , Ruiz Montesinos I , Herrero de la Parte B 
Published: January 2023

Abstract

Introduction
Despite the known cytotoxic and hepatotoxic effects of the most common chemotherapy drugs, their use in the context of liver regeneration as part of antitumor treatment is controversial.

Methods
Thirty-six WAG/RijHsd rats were induced to selective portal ligation of 50% of the hepatic parenchyma, and divided into 3 experimental groups. 72 hours before, two of the groups were treated with 5-Fluorouracil (50mg/kg) (5FU) or with 5-FU (50mg/kg) + Oxaliplatin (10mg/kg) (5-FU+OX); the third was the untreated control. The weight of the animals and the liver remnant were quantified and the biochemical profile was determined after 24 hours (half of the animals) or 7 days (the other half).

Results
The treated animals did not show differences in the percentage of liver remnant after 24h or 7 days (control: 67±3.5; 5-FU: 66±2.9; 5-FU+OX: 65±1.1%). After 24 hours, an increase in AST and ALT was detected in the treated groups (5-FU and 5-FU+OX) compared to the control (1783±110 vs. 2306±516 vs. 1400±273 IU/L; and 1251 ±121 vs. 1822±386 vs. 949±145 IU/l, respectively, p&lt;0.05). After 7 days, slight differences remained, but were not statistically significant. Albumin was significantly altered throughout the experimental process, without normalizing after 7 days.

Conclusion
The combined 5FU+OX treatment does not affect the regenerated liver volume, although it triggers an initial cytolytic reaction, which normalizes over time, with the nutritional deficit persisting.
AUTONOMOUS SPERMATOZOON-LIKE MICROROBOT WITH CHEMICAL PROPULSION
Authors: Mancha Sánchez E , Serrano Balbontín A J , Tejado Balsera I , Vinagre Jara B 
Published: January 2023

Abstract

Introduction
Robotics, as one of today's fastest growing disciplines and in support of surgeons, can generate significant advantages for both doctors and patients, such as increased automation of routine procedures or the performance of previously impossible procedures. Microrobotics is the use of microscale robots to be introduced into the body. The aim of this study is to develop a prototype of an autonomous spermatozoon-like microrobot propelled by chemical reactions for navigation in lumens.

Methods
To study the propulsion of the microrobot, a simulation was carried out in COMSOL. The movement of the microrobot was simulated by applying alternating forces emulating chemical actuation with and without a passive flagellum, in a fluid environment. In addition, a macro-scale prototype was developed. Two motors were used for propulsion with attached propellers. Their speed and the development of the programmed functions for movement were monitored. The microrobot's movement, propulsion and flagellum effect were studied in water.

Results
The simulations showed that without the flagellum, a drifting movement was generated, stabilising with its presence, as well as adding an additional impulse. The prototype showed similar behaviour to that obtained in the simulations, moving in the desired way.

Conclusions
It has been shown that the presence of the flagellum, without actuation, generates an additional impulse to the one developed by the initial propulsion. The prototype has also shown the feasibility of the simulations, and the possibility of manufacturing a microrobot on a sub-millimetre scale.
CORRELATION BETWEEN PATHOLOGIC AND INTRAOPERATIVE FINDINGS IN PATIENTS UNDERGOING PERITONEAL ONCOLOGIC SURGERY
Authors: García Alonso L , Rodríguez Uría R , González Turienzo E , Sanz Navarro S , Díaz Vico T , Contreras Saiz E , Turienzo Santos E O , Sanz Álvarez L 
Published: January 2023

Abstract

Introduction
Surgical Peritoneal Carcinomatosis Index (PCIq) subjectively calculated during surgery is a universally accepted value, both selective and prognostic. Calculation of PCI based on anatomopathological findings (PCIp) could be a more reliable value of tumor burden. We compare the differences between PCIq and PCIp by evaluating the prognostic significance and usefulness of PCIp.

Methods
We conducted a retrospective study of patients undergoing cytoreduction surgery and HIPEC between 2015 and 2019 in our General Surgery Department. Resected specimens were classified according to their definitive pathological anatomy for the calculation of PCIp, and compared with PCIq for each patient. ROC curves were applied to compare the prognostic value of both PCIs.

Results
Within the 68 procedures, the difference between PCIq and PCIp was 1.19 (0–8), with a total concordance in 53% of the cases. The discordance did not depend on the type of tumor, previous surgeries or neoadjuvant treatment. In 75% of the procedures there were negative samples for malignancy, which was associated with a higher disease-free survival (p = 0.017). ROC curves showed that PCIp was similar to PCIq as a predictor of survival or recurrence, with the number of positive resected specimens being the best predictor of recurrence (p = 0.035).

Conclusions
PCIq overestimates tumor burden, as the presence of negative specimens is frequent. PCIp does not seem to be a better tool for predicting recurrence or survival in our series. The number of positive samples more accurately estimates the probability of recurrence than PCIq, which may have prognostic significance.
PREHABILITATION THROUGH A MOBILE APPLICATION (PREHAPP) IN MAJOR COLON CANCER SURGERY
Authors: Lillo-García C , Lario Pérez S , Sánchez-Guillén L , Quinto Llopis S , Soler-Silva Á , Alcaide Quirós M J , López Rodríguez-Arias F , Arroyo A 
Published: January 2023

Abstract

Introduction
Prehabilitation aims to optimise functional capacity before surgery. We present the prehabilitation programme through a mobile app (PREHapp).

Methods
Patients with resectable colon cancer, ≥18 years old, without comorbidities and with Android Smartphones are selected. The following programme is proposed through PREHapp during the 4 week pre- and postoperative period. Depending on the results of the 6-minute test and the initial ergospirometry, the programme would be individualised with different objectives.
The app allows the patient to record their activities and synchronises with a website designed for the surgeon through which he/she can monitor compliance.

Results
The impact of prehabilitation on outcomes depended on compliance. Studies show that high compliance rates in ERAS programmes are associated with fewer postoperative complications. In this aspect, telemedicine could have great potential, as it has enabled the design of new health programmes that improve accessibility, including the creation of apps. The use of these apps favours therapeutic compliance and patient education, which enhances their empowerment in disease management.

Conclusions
There are few studies on the use of telemedicine in the field of prehabilitation. The PREHapp application could improve compliance and thus improve functional capacity before surgery.
PRELIMINARY RESULTS OF lncRNAs AS BIOMARKERS FOR COLORECTAL CANCER DIAGNOSIS
Authors: Fernández Hevia M , Fernández Martínez D , Cobzariu M A , García Santos G , Díaz Vico T , Miranda Castro R , Lobo Castañón M J , García Flórez L J 
Published: January 2023

Abstract

Introduction
lncRNAs are strands larger than 200 nucleotides that do not encode proteins. They have regulatory functions in cell proliferation, apoptosis and migration. They are stable over a long period and may have diagnostic value in colorectal cancer (CRC). Their availability as effective biomarkers for CRC screening would represent a substantial advance, as tests could be performed with minimal risk and wide acceptance. The aim of this study is to evaluate selected lncRNAs in plasma as potential diagnostic biomarkers in CRC.

Methods
Prospective cohort study between patients operated on for CRC (cases) and healthy patients (controls). Blood samples were processed in the biobank to obtain plasma by centrifugation. Subsequently, the levels of three CRC-related lncRNAs (HOTAIR, CCAT2 and PVT1) were determined in the laboratory of the Electroanalysis Group of the University of Oviedo (GEUO).

Results
In the initial results, an overexpression of the three genes (HOTAIR, CCAT2 and PVT1) was observed in CRC patients, with HOTAIR being the one that best differentiates between CRC and healthy patients. In addition, the combined determination of the three lncRNAs could obtain better results than the individual determination of each of them.

Conclusions
The lncRNAs studied are overexpressed in the CRC patient sample. The sample size needs to be increased to confirm this trend. It is logical to postulate that molecular technologies will lead to the development of better screening modalities and individualised treatments.
SMALL BOWEL INTUSSUSCEPTION CAUSED BY METASTASIS FROM A LOWER LIMB LIPOSARCOMA
Authors: Soto Dopazo M , Arango Bravo A , Lora Cumplido P , Nuño Iglesias C , Mateos Palacios C , Antuña Villa S , Lezana Pérez M Á , Fernández Fernández J C 
Published: January 2023

Abstract

Introduction
Liposarcoma represents one of the most frequent types of soft tissue sarcomas, constituting a heterogeneous group of lesions. There are four different subtypes: well-differentiated; dedifferentiated; myxoid and pleomorphic. Its main locations are the retroperitoneum, extremities, trunk and the cervical area.

Clinical case
A 90-year-old male with a pleomorphic liposarcoma in the left thigh in 2012, local recurrence from a dedifferentiated liposarcoma in 2018 and 2021, presented to our emergency department with abdominal pain and vomiting. CT scan revealed an image of intestinal intussusception in the distal jejunum due to a 5 cm intraluminal polyp.
Emergency laparotomy revealed a change in the distal jejunum caliber caused by the intussusception finding a 5 cm polypoid mass. We made an intestinal resection with a L-L anastomosis. Histopathological examination showed evidence of metastasis from dedifferentiated liposarcoma.

Discussion
A sarcoma is a malignant tumor, an infrequent type of cancer from connective tissue. The liposarcoma is the most frequent subtype and the dedifferentiated type can occur as dedifferentiation from other subtypes. The most common sites of metastasis include the lung, liver, and bone. Metastasis to the small bowel is rare with few reported cases.
Intestinal intussusception is an infrequent cause of mechanical intestinal obstruction in adults. Etiology is diverse and it is often caused by benign lesions in the small bowel and malignant lesions in the colon. The diagnostic technique of choice is the CT scan with contrast and treatment usually requires surgery. Intestinal reduction is controversial because of the risk of perforation or tumor spread.
MANAGEMENT OF HEMORRHAGE IN LAPAROSCOPIC COLORECTAL SURGERY
Authors: Silva Cano D W , López-Negrete Cueto E , Rodríguez García J I , Fernández Martínez D , Ibero Casadiego G P , García Alonso L , García Flórez L J , Granero Trancón J E 
Published: January 2023

Abstract

Introduction
Laparoscopic colorectal surgery (LCRS) offers similar oncologic outcomes to the open approach along with early postoperative recovery but is not without complications, with bleeding being uncommon but with variable impact and technical resource demand.

Methods
A descriptive and retrospective study of bleeding in patients undergoing elective LCRS in our center between 01/012018 and 31/12/2021. The data analysis was performed using the SPSS statistical software.

Results
Out of 945 LCRS, 45 patients (4.8%) presented some hemorrhagic event. The main diagnosis was colorectal cancer (35), followed by diverticular disease (6). The procedures most frequently associated with bleeding were sigmoidectomy (13), TATME (7) and right hemicolectomy (7). Five patients presented moderate (4) or severe (1) intraoperative bleeding, controlled during surgery. The most commonly used hemostatic devices were biopolar energy (29.5%) and combined (34.1%). The diagnosis of postoperative bleeding was clinical in 20 cases (45.5%), 17 by CT angiography (38.6%) and 7 by endoscopy (15.9%). In 36.4% the origin of hemorrhage was in the anastomosis, demonstrated by endoscopy or radiological study. Eleven patients (25%) required reintervention: seven by open approach and four by laparoscopy (two conversions). Therapeutic endoscopy was performed in 7 patients (15.9%), in 1 patient interventional radiology-surgery was combined, and in another patient endoscopy-surgery. There was no mortality due to this cause. The mean length of stay after surgery was 19.52 days.

Conclusions
Hemorrhage in LCRS is a rare but potentially serious complication that requires early diagnosis and staged multidisciplinary approaches, including advanced therapeutic endoscopy and interventional radiology procedures.
DEVELOPMENT OF A NATURAL POLYMER-BASED HYDROGEL FOR BIOENGINEERED VASCULAR GRAFTS
Authors: Loureiro J , Miguel S P , Galván-Chacón V P , Patrocinio D , Sánchez-Margallo F M , Pagador J B , Ribeiro M P , Coutinho P 
Published: January 2023

Abstract

Introduction
Cardiovascular diseases are a main cause of death globally, and their treatment implies various vascular repairs through different techniques like angioplasty, stent placement in the blocked artery, or bypass surgery. Artificial grafts would significantly reduce the number of non-treated patients, but middle and long-term failures compromise their clinical use.

Methods
Herein, we developed a hydrogel composed of gellan gum, gelatin, and sodium alginate for bioengineered vascular graft production. The vascular grafts were characterized by their swelling, porosity, biodegradability, and cytotoxic profile.

Results
The bioengineered materials were easily assembled due to the thermoresponsive nature of the hydrogel and had a vessel-like structure resembling the native vasculature. These vessels had a very controlled swelling degree, and notably, the hydrogel structure was stable and maintained its morphology. The vascular grafts had a porosity of 82.6 ± 4.3% and exhibited a controlled biodegradation rate with a maximum of 24.2 ± 3.0%. As expected, the natural materials used showed no cytotoxicity toward HUVECs cells since they are natural polymers described as biocompatible.

Conclusions
This developed natural hydrogel showed promising potential to be used to develop bioengineered vascular grafts.
INITIAL EVALUATION OF A REAL-TIME ERGONOMIC POSTURE ASSESSMENT SYSTEM IN LAPAROSCOPIC SURGERY
Authors: González Nuño F M , Plaza de Miguel C , Sánchez Margallo J A , López Agudelo I , González Portillo M R , Sánchez Margallo F M 
Published: January 2023

Abstract

Introduction
The objective of this study is to perform an initial evaluation of a system for assessment and visual feedback of the surgeon's posture during laparoscopic practice.

Methods
In order to monitor the surgeon's posture, we used the XSens motion tracking system. The neck, shoulder, elbow, and wrist joints were analyzed during laparoscopic practice. A visual feedback method was designed using Unity3D which indicates in real time if any of the analyzed joints suffers from an inadequate posture, allowing for its possible correction. The system was evaluated with a group of seven novice laparoscopic surgeons who performed several repetitions of two basic training tasks (eye-hand coordination and transfer). The participants were randomly organized into group A (4 subjects), who received visual feedback, and group B (3 subjects), as a control group.

Results
Regarding the coordination task, group A exhibited lower ergonomically inappropriate posture time for the left shoulder and elbow, being significant for the left shoulder posture. Similarly, this group showed a positive evolution during the training process for the different joints. Regarding the transfer task, both groups presented a positive evolution.

Conclusions
Preliminary results show that the presented method allows novice surgeons to improve their posture during laparoscopic practice. The use of the non-dominant hand and the complexity of the task seem to be conditioning aspects in the surgeon's ergonomics.
ROBOTIC PLATFORMS FOR MICROSURGERY: A SYSTEMATIC REVIEW
Authors: Pires Louçã L C , Jara Muriel M J , Plaza de Miguel C , González Francisco M , Abellán Rubio E , Sánchez Margallo J A , Sánchez Margallo F M 
Published: January 2023

Abstract

Introduction
Microsurgery involves procedures that demand maximum precision and dexterity, as well as the absence of tremor. In this regard, new robotic-assisted microsurgery solutions have emerged in recent years. The objective of this review is to analyze the current status of the robotic platforms in microsurgery.

Methods
This systematic review has been focused on the PubMed database over the last seven years (2015–2022), using keywords related to robotic platforms for microsurgery. Articles including clinical/preclinical studies were considered, and non-English language articles, abstracts, conference proceedings, reviews and letters were excluded.

Results
A total of 324 articles were identified, 27 of which passed the eligibility criteria.
Of the 14 platforms analyzed, da Vinci, MM3 and the easyMicro prototype incorporate their own vision system. The majority of the systems have tremor filtering and provide haptic feedback. The da Vinci system is the most widely used system in vascular and nerve microsurgery, reconstructive and lymph node dissection, eye surgery and urology. The MUSA and Symani systems have been used in reconstructive supermicrosurgery, the neuroarm in neurosurgery, the Surgical Robotic System and the easyMicro prototype in reconstructive and ocular surgery. The MM-3 (vascular), SMART (ocular) and smartARM (neurosurgery) have been tested in artificial models.

Conclusions
Among the platforms analyzed, da Vinci was the most widely used, specifically in ocular and reconstructive surgery. MUSA and neuroARM are rapidly progressing in reconstructive microsurgery and neurosurgery, respectively.