bluuebunny/crossref_metadata_2025_split huggingface dataset:
Centriselect – A novel approach for centrifugal cell separation
Authors: Behrens Stephan , Hähne Philipp , Schmieder Peter , Schmieder Florian 
Published: September 2023

Abstract
The separation of bio-suspensions, such as blood, is of great interest for many biomedical applications, such as the extraction of leucocytes from whole blood, the laboratory analysis of specific cells or a mixed cell suspensions from bioreactors or the preparation of cellular products for cellbased therapy. In particular, mechanical separation processes have been established for the rapid separation of biosuspensions. The principle of mechanical separation is based on the specific density of the particles or the particle size. Thus, the corresponding processes are based on centrifugation and/or filtration. The standard process to prepare blood products from whole blood donations contains several steps. First the blood is separated into different phases by centrifugation and then further separated by filters containing specific filtration layers. The shortcomings of this process is the low yield due to bio-particles remaining in the filter and the high costs for equipment due to the use of multiple devices for centrifugation and filtration. Here we present the proof of principle of an adaptive, size-selective filter that addresses the above mentioned shortcomings. The filter is designed to combine the filtration and centrifugation process within one step. Therefore a novel filtration device was designed that can be used within a standard centrifugation tube. During centrifugation, the centrifugal force changes the filtration coefficient of the filter. This can be used to separate different sized of bio-particles at different centrifugation speeds. Moreover, the cells that were hold back in the filter can be removed afterwards by increasing the centrifugation speed. In this way, the filter is created to gradually release cells of a specific size yielding in a high purity of different cell fractions.
Core-Shell-Nanoparticles with Superparamagnetic Properties for Novel Applications as Biomaterials
Authors: Hagemann Valentin , Finck Laura , Herrmann Timo , Menzel Henning , Ehlert Nina 
Published: September 2023

Abstract
Due to the increasing average age of the population, the number of implants is also increasing and with it the number of explantations. Therefore, facilitated implant removal is of great interest. A nanocomposite consisting of superparamagnetic core-shell nanoparticles (CSNPs) and a synthetic polymer is supposed to be used as implant coating, aiming for a stimulus-inducible modification of the composite’s rheological properties by hyperthermia. Here, the first steps following this concept, the synthesis and modification of the CSNP are reported. In this work magnetite nanoparticles build the core and are surrounded by a periodic mesoporous organisilica (PMO) shell. For this reason, the CSNP are referred to as magnetic PMO (mPMO) particles in the following.
Acoustic Emission Analysis (AEA) in unilateral leg amputees as a future quality tool in orthopaedic technology to evaluate the adjusted prosthesis in relation to gonarthritis
Authors: Subke Joerg , Keller Adeline , Hermawan Hermawan , Schmeiler Michael , Schneider Benedict , Schwalbe Hans-Joachim 
Published: September 2023

Abstract
In orthopaedic technology unilateral leg amputees are provided with prosthesis which are attached through a shaft to the remaining part of the leg. The prosthesis legs are built according to manufacturer’s specifications and get adjusted to the individual needs through motion analysis. If the prosthesis is not adjusted correctly to the individual needs of the user, the chance for development of arthritis in the remaining knee joint is greatly increased. The goal of this paper is to develop a procedure based on AEA to detect signs of arthritis in the remaining knee joint early and the base of a procedure for a future assessment of the quality of the care. The measurement system consists of the AEA - measurement device, a force plate to measure ground reaction forces, two strain gauges which are attached to handles on a test rig and two video cameras which record the movement from frontal and sagittal view. The amputee performs a standard movement in the test rig which consists of three knee bends in ten seconds while wearing the prosthesis legs. The test rig is provided so the amputee can support themselves using arms and hands as prosthesis’ support during the extension phase of the knee bend. The posture and load of the knee joint during the movement is analyzed with the video and force data. The ground reaction forces show the load distribution between the remaining leg and the prosthesis and its support during the movement. The hand forces give clues how well the amputee can balance and which arm is favored under load. By combining kinematic data and AEA the state of the load transfer zones can be evaluated. Thus, it’s possible to examine the remaining knee joint for defects and evaluate the quality of the prosthesis adjustment.
The effect of fiber content and aspect ratio on anisotropic flow front and fiber orientation for injection-molded fiber composites
Authors: Tseng Huan-Chang 
Published: September 2023

Abstract
In state-of-the-art predictive engineering software for injection molded fiber composites, it is challenging to discuss the effect of fiber properties, including fiber content and aspect ratio, on the shell-core fiber orientation and anisotropic flow front. Recently, the flow-fiber coupled model of informed isotropic (IISO) viscosity was proposed for simulating the realistic flow-induced fiber orientation. At present, one attempts to improve the IISO viscosity in relation to fiber content and aspect ratio. Therefore, injection molding simulations of 30 wt% and 50 wt% short glass fiber-reinforced Polyamide66 (30 wt% SGF/PA66 and 50 wt% SGF/PA66) are performed in the IISO flow-fiber couple 3D-FVM (three-dimensional finite volume method) computation under the fixed aspect ratio of a

r
= 20. As a result, the predicted fiber orientation distributions are in good agreement with related experimental data, while the core width is increased with fiber contents. In addition, the anisotropic ear flow occurs at the higher content. Based on shear viscosity of 30 wt% SGF/PA66, one can directly alter fiber content as 50 wt% SGF/PA66 in the IISO computation to anticipate the reliable trend of fiber orientation and flow behavior. When the a

r
= 20 short fiber is lengthened as the a

r
= 50 long fiber, the core obviously becomes the wider. It is significant to investigate the difference between the decoupled and coupled computation, as well as the influence of the flow-fiber coupled and fiber orientation model parameters.
Experimental studies on water absorption and mechanical properties of Hibiscus
sabdariffa (Roselle) and Urena
lobata (Caesar weed) plant Fiber–Reinforced hybrid epoxy composites: effect of weight fraction of nano-graphene fillers
Authors: Sasi Kumar Mani , Sathish Selvaraj , Makeshkumar Mani , Gokulkumar Sivanantham 
Published: September 2023

Abstract
This study aimed to develop novel hybrid composites with graphene (Gr) fillers incorporated in the epoxy (E) matrix with Caesar weed fiber (CF), and roselle fiber (RF) as reinforcements. Compression molding methods were used to fabricate hybrid composite materials with a variable-weight graphene filler in a constant fiber epoxy matrix. On the basis of the results, the mechanical characteristics of the composite with 6 wt% Gr exhibited the greatest flexural strength, tensile strength, and impact strength. This occurred because 6 wt% Gr particles are more uniformly dispersed in an epoxy matrix, resulting in better compatibility between reinforcementand matrix, thus increasing the mechanical properties. The composite with 8 wt% Gr filler reinforcement had the maximum hardness rating and the lowest percentage of water absorption. According to the results, adding graphene fillers to the CF/RF/E composite significantly improved the mechanical and water absorption performances. Scanning electron microscopy was used to examine the surfaces of the fabricated samples. The weight fraction of the graphene filler was optimized to enhance the mechanical properties of the composite for use in various engineering applications, such as automobile, defense, marine, sports, and musical instruments.
Can preoperative neopterin levels predict acute kidney injury in patients undergoing on-pump cardiac surgery?
Authors: Çiçek Ömer Faruk , Akyürek Fikret , Akbayrak Hakan , Orhan Atilla , Kaya Eyüp Cihan , Büyükateş Mustafa 
Published: September 2023

Abstract

Objectives
The aim of this study was to investigate the potential of preoperative neopterin levels as a predictive marker for postoperative acute kidney injury (AKI) in patients undergoing on-pump cardiac surgery, in addition to other potential risk factors.

Methods
This observational study included 91 patients who underwent elective cardiac surgery under cardiopulmonary bypass. Of these, 35 patients (38.46 %) experienced AKI following surgery, as outlined by the Kidney Disease Improving Global Outcomes (KDIGO) standards. The study participants were divided into two groups depending on whether they had developed AKI after the surgery or not. The study compared two groups and utilized logistic regression analysis to evaluate potential predictors. A receiver operating characteristic (ROC) analysis was conducted to determine the ability of preoperative neopterin levels to predict the occurrence of AKI.

Results
A comparison of the baseline demographic, clinical, laboratory, and echocardiographic characteristics was conducted between patients who suffered from AKI and those who did not. The multivariate analysis demonstrated that EuroSCORE II (OR, 4.525; 95 % CI, 1.29–15.87; p=0.019), X-clamp time (OR, 1.157; 95 % CI, 1.01–1.326; p=0.035), and neopterin levels (OR, 22.952; 95 % CI, 3.14–167.763; p=0.002) were independently predicted the post-cardiac surgery AKI. ROC analysis identified a cut-off value of 9.65 nmol/L, which had a sensitivity of 91.4 % and a specificity of 91.1 % (area under the curve, 0.98; 95 % CI, 0.958–1; p<0.001).

Conclusions
Our study emphasizes the potential of preoperative neopterin levels, EuroSCORE II, and X-clamp time as independent predictors of postoperative AKI, even in milder cases, in individuals undergoing on-pump cardiac surgery.
A Scoping Review of the Physiological Profiles of Motorsport Drivers: Implications for Athlete Training
Authors: Beaumont Patrick L. , van den Hoek Daniel , Holland Justin , Garrett Joel 
Published: September 2023

ABSTRACT
Automotive racing is one of the most internationally recognized sports. Drivers within their respective disciplines are exposed to a variety of forces throughout the race, each of which is theorized to inflict a physiological adaptation within the driver. Therefore, the aim of this scoping review was to collate the evidence and provide a summary of the physiological profiles of these drivers and provide practitioners with recommendations to optimize these adaptations. Of the 1,304 articles screened, 24 met the inclusion criteria. Of the included studies, 13 reported on the anthropometric and physiological measures of 4-wheeled racing athletes from both open and closed-wheel disciplines. The remaining 11 studies reported on the physiological profiles of 2-wheeled racing athletes from both on-road and off-road riding disciplines. Open-wheel racers have greater cardiopulmonary fitness than close-wheel racers, as well as greater strength throughout movements of neck lateral flexion and isometric knee extension. Close-wheel racers were shown to be heavier with greater fat mass than the open-wheel racers but exhibited greater isometric shoulder flexion and plantarflexion strength. Female 2-wheeled racers were also shown to have a weaker handgrip strength than their male counterparts. Whereas male on-road riders demonstrated greater levels of handgrip strength than their off-road counterparts.
Mouse oocytes restore antral stage partial mechanical denudation in vitro
Authors: Herta Anamaria-Cristina , Akin Nazli , Segers Ingrid , Billooye Katy , Smitz Johan , Anckaert Ellen 
Published: September 2023

In brief
Partially denuded mouse cumulus–oocyte complexes restore likely functional transzonal projections in culture, under meiotic inhibition, with no detectable impact on oocyte competence. This proof-of-concept study constitutes positive premises for improving the developmental competence of human capacitation (CAPA)–in vitro maturation (IVM) oocytes with inadequate somatic cell connections.

Abstract
In vitro oocyte culture might be the sole option for fertility preservation in some patients. This relies on constant oocyte–somatic bidirectional communication, and its precocious disruption alters oocyte competence. In non-human chorionic gonadotropin-triggered human in vitro maturation (IVM), retrieval of cumulus–oocyte complexes (COCs) by needle aspiration from the targeted small follicles (2–8 mm) leads to the collection of some partially denuded (PD) COCs with poor developmental competence. Hypothetically, re-establishing connectivity in these COCs could rescue oocyte quality. To test this, we used a well-characterized mouse preantral follicle culture system. On day 8, at antral stage, in part of the follicles, the oocytes were mechanically denuded while in other follicles in vitro grown oocytes were replaced with age matched fully stripped in vivo grown ones. The denuded oocytes were cultured on top of the somatic compartment until day 12, when oocyte–somatic reconnection was assessed. Furthermore, to better mimic the current biphasic IVM setup, fully surrounded (FS) COCs were collected from 19- to 21- day-old unprimed mice. Following partial mechanical denudation, COCs were cultured under meiotic inhibition for 2–4 days, to test oocyte–cumulus cell (CC) reconnection. Meiotic and developmental competence endpoints were compared between reconnected and FS-cultured COCs. We concluded that (i) in vivo- and in vitro- grown antral oocytes reconnect with in vitro-grown somatic companions; (ii) PD–COCs restore the FS morphology in culture, under meiotic inhibition; and (iii) oocyte quality from reconnected and intact cultured COCs is comparable. These observations encourage translational work to rescue partially denuded oocytes in human IVM.
Chemotranscriptomic analysis of 7-hydroxymatairesinol-related effects on MCF7 human breast tumor cells
Authors: Torshin I. Yu. , Rubashkina A. N. , Gromova O. A. 
Published: September 2023

Introduction. Establishing the effects of anticancer drugs on the transcriptome is an important procedure in postgenomic pharmacology necessary to comprehensively assess the desired and undesirable effects of candidate drugs. Aim: to assess the effects of lignan 7-hydroxymatairesinol (7HMR) on breast tumor cells.Materials and Methods. Chemotranscriptome profiling was carried out in MCF7 cells (breast cancer cell line) after 24-hour incubation with 7HMR. The GEO (Gene Expression Omnibus) database contains samples of data from transcriptomic studies allowing to model dose-dependent compound-related effects on gene expression based on the chemograph-derived combinatorial analysis algorithms. As a result, a panel of genes with altered expression is generated, which are analyzed by the functional linkage method using the international nomenclature of Gene Ontology (GO) linked to biological roles of genes/proteins.Results. Dose-dependent effects of 7HMR on gene transcription (change in transcription by 5 % or more per 1 μmol 7HMR) were established for 3,468 out of 12,700 genes studied. 7HMR significantly reduced the expression of genes involved in maintaining cell proliferation (401 genes including those involved in telomere maintenance), protein synthesis (194 genes) and proteasomal protein degradation (70 genes), energy metabolism in tumor cells (91 genes) and chronic inflammation (148 genes). A decline in the expression of such gene groups retards the processes of proliferation and vital activity along with protecting host body from excessive inflammation. 7HMR contributed to a predominant increase in the transcription of gene groups involved in antitumor activity (more than 100 genes), including the genes involved in maintaining antitumor immunity as well as those mediating the antitumor effects of vitamin D, retinoids, and vitamin C.Conclusion. The revealed changes in gene transcription enhance 7HMR-related effects on proteome proteins and suggest the prospects for using 7HMR for effective and safe prevention and treatment of nodular mastopathy and breast cancer.
NOD-like receptors in pathogenesis of missed and spontaneous abortions
Authors: Lebedeva O. P. , Ivannikova V. M. , Zhukova I. O. , Kozarenko O. N. , Altukhova O. B. , Pakhomov S. P. , Churnosov M. I. 
Published: September 2023

Nucleotide-binding oligomerization domain-like receptors (NOD-like receptors) are cytosolic signaling receptors of innate immune cells recognizing ligands derived from bacteria, viruses, fungi and protozoa. They can initiate apoptosis and pro-inflammatory cytokines production. Meanwhile, the role of decidual NOD-like receptors in pathogenesis of early miscarriages remains unknown. Aim: to study NOD-like receptor (NOD1, NOD2, NLRP1, NLRP3, NLRC4) messenger ribonucleic acid (mRNA) expression in decidual tissue from patients with missedand spontaneous abortions compared to progressive pregnancy.Materials and Methods. NOD1, NOD2, NLRP1, NLRP3, NLRC4 and pathway protein receptorinteracting-serine/threonine-protein kinase 2 (RIP-2) mRNA expression in decidua from 34 patients with missed abortions (group I), 34 patients with spontaneous abortions (group II) and 57 women with progressive pregnancy admitted for artificial abortion (group III, control group) were analyzed by reverse transcription quantitative polymerase chain reaction (PCR) at gestational age of 6–10 weeks. Exclusion criteria were as follows: endocrine disorders, severe extragenital diseases, antiphospholipid syndrome, inherited thrombophilia, uterine malformations and fetal chromosomal abnormalities. Samples were collected by uterine abrasion. Results. It was found that mRNA expression of NOD2 was significantly higher in decidua from patients with missed and spontaneous abortions, whereas for RIP-2 (related to relevant signaling pathway) – in women with missed abortions. A moderate positive correlation between gestational age and mRNA expression for NOD2 (R = 0.48; p = 0.01) and RIP-2 (R = 0.41; p = 0.007) was observed in subjects with progressive pregnancy. In contrast, women with missed abortions showed a moderate negative correlation between body weight and mRNA expression for NOD2 (R = –0.46; p = 0.03) and RIP-2 (R = –0.51; p = 0.02) whereas spontaneous abortions was associated with moderate negative correlation between RIP-2 mRNA expression and body weight (R= –0.47; p=0.04) as well as body mass index (R= –0.48; p = 0.04) along with moderate positive correlation with age of menarche (R = 0.46; p = 0.04). However, compared with progressive pregnancy no significant differences were found in expression level form NOD1, NLRP1, NLRP3 and NLRC4 mRNA in decidua from patients with missed and spontaneous abortions. Conclusion. Elevated NOD2 mRNA expression was observed in decidua from patients with missed and spontaneous abortions compared to progressive pregnancy paralleled with upregulated RIP-2 mRNA expression in missed abortions. Finally, it was found that NOD1, NLRP1, NLRP3 and NLRC4 were not involved in pathogenesis of miscarriages.
COVID-19 Perceptions Among North Carolina Native Americans: Using a Qualitative Approach
Authors: Shi Xinyan , Mehng SiAhn , Locklear Alice Kay , Kang Zhixin , Locklear Tracie , Locklear Mary Beth , Jacobs Mary Ann , Maynor Tammy , Gao Guanlin , Kumar Deepak 
Published: September 2023

Background COVID-19 has amplified the health and economic disparities in Native American communities. However, there are limited data from Native American populations. This study is the first phase of the Building Resilience And Vital Equity (BRAVE) project, the scope of which is to understand Indigenous perceptions, attitudes, and behaviors about COVID-19 and design and implement culturally sensitive interventions to increase testing and vaccinations among Native American communities. Method We recruited 26 Native Americans and conducted open-ended, semi-structured interviews to explore participants’ perceptions of COVID-19 and related themes, such as needed support, informational sources, trust, communication, and protection of elders. Results The results show that wearing a mask, hand sanitizing, and social distancing had very high support while perceptions of testing and vaccination decisions were mixed. Additionally, two themes stand out: the lack of reliable information resources to learn about COVID-19 and the lack of trust in the government. Limitation This study included a small sample size, which makes it difficult to include participants from diverse demographic backgrounds. Lack of familiarity with the online meeting format and the limited internet access in some indigenous communities may have prevented some people from participating. Conclusion The findings confirmed the need to improve existing public health information infrastructure and helped explain the intensified impact of COVID-19 on the Indigenous marginalized community. The findings help identify the key factors that affect Native Americans’ testing and vaccination decisions and provide guidance on the designs of community intervention programs.
COVID-19 e saúde mental: preocupações de estudantes do ensino superior do estado do Ceará
Authors: Oliveira Eliany Nazaré , Pereira Paulo , de Almeida Paulo César , Regino Oliveira Flávia , Petra Mororó Ziesemer Ravena , Monte Lima Muniz Lidiane , Monteiro da Silva Lídia Cristina , Cavalcante Costa João Breno , Ferreira Lima Gleisson 
Published: September 2023

Objetivo: Objetivou-se apresentar as principais preocupações de estudantes do ensino superior do estado do Ceará, durante a pandemia da COVID-19. Metodologia: Trata-se de recorte de pesquisa maior denominada “Repercussões da pandemia do novo coronavírus na saúde mental dos estudantes de ensino superior”. Estudo descritivo e exploratório que utilizou abordagem transversal. A pesquisa contou com amostra de 3.691 alunos que participaram do estudo. Devido ao contexto da pandemia, a coleta de dados foi realizada por meio remoto, via Google Forms. Resultados: O estado de Saúde Mental, de forma global, as dimensões Bem-Estar Positivo e Distresse e as dimensões primárias apresentaram tendência para diminuir com o aumento da preocupação com a continuidade do curso de forma presencial. O estudo apresentou insegurança por parte dos estudantes universitários quanto ao retorno das aulas e de como seria o “novo normal”. Quanto ao medo de ser infectado e em relação à preocupação ao sair de casa, respectivamente, 76,72% e 84,85% apresentaram estado de saúde mental inferior, demonstrando, assim, como a pandemia fragilizou a saúde mental. Conclusão: Os resultados apresentaram validação estatisticamente significativas tanto para a Escala global quanto para as dimensões primárias. À vista desse panorama, constata-se o quanto a pandemia e o isolamento social repercutiram em emoções de medo e preocupação, estes sentimentos interferiram negativamente na saúde mental dos estudantes pesquisados.