bluuebunny/crossref_metadata_2025_split huggingface dataset:
Performance of the Sysmex XN‐V hematology analyzer in determining the immature platelet fraction in dogs: A preliminary study and reference values
Authors: Jornet‐Rius Oriol , Mesalles‐Naranjo Montse , Pastor Josep 
Published: July 2023

AbstractBackgroundImmature platelets (IPs) are newly formed platelets released into circulation that have been demonstrated as good markers of thrombopoiesis. Although many flow cytometric and fully automated‐based methods are available, the latest Sysmex XN‐V hematology analyzer for veterinary use is equipped with a specific fluorescent platelet channel (PLT‐F) that detects platelets using a platelet‐specific dye.ObjectivesThe aims of this study were to evaluate the performance of the Sysmex XSN‐1000 V in determining the IPF (immature platelet fraction) and other selected PLT‐F channel parameters and to propose IPF reference intervals (RIs) for canine blood samples.MethodsCanine EDTA blood samples were analyzed on the Sysmex XN‐1000 V to assess linearity, imprecision, carryover, stability, and the effect of platelet clumping on selected platelet parameters from the PLT‐F channel. We also reported the de novo generated RIs for the IPF in dogs.ResultsImprecision was acceptable (CV <10%) for all parameters except for the absolute IPF values (IPF#), in which the reproducibility was 12.15% for the normal‐low concentration samples. Linearity and carryover were excellent for all variables. Relative IPF values (IPF %) and IPF# remained stable for both storage conditions for up to 48 hours; however, a nonsignificant progressive increase in these parameters was observed from 12 hours at 4°C. We observed a statistical increase in IPF% and IPF# and a statistically significant decrease in PLT‐F counts after intentional in vitro platelet aggregation. RIs were generated for all reference samples (n = 69) and for samples with (n = 25) or without (n = 44) platelet clumps.ConclusionsThe performance of the new PLT‐F channel‐derived variables for dogs was excellent. Specific RIs for IPF should be used when platelet aggregates are present.
Methodology‐related pseudohyperkalemia associated with marked muscle enzyme leakage in a dog
Authors: Clancey Noel , Burton Shelley , Gilroy Cornelia , Saunders Janet 
Published: July 2023

AbstractBackgroundAs hyperkalemia may be life‐threatening, it is critical to recognize artifactually increased potassium concentrations. Pseudohyperkalemia may occur in myopathies when using the VetScan2 analyzer (VS2), but the degree of pseudohyperkalemia and relationships relative to creatine kinase activity (CK) are unknown.ObjectivesWe aimed to determine what degree of muscle enzyme leakage, as reflected by increased serum CK activity, results in cases with falsely elevated potassium concentrations when measured by the VS2. We also sought to establish if a linear relationship exists between potassium concentrations measured by the VS2 and CK activity.MethodsSerum samples from dogs with increased CK activity and with CK activity within the reference interval and without clinically relevant biochemical alterations were used to create diluted samples having various CK activities. Potassium concentrations and CK activities were determined on VS2 and Cobas c501 (Cobas) analyzers. Wilcoxon signed rank, Bland–Altman, and Passing‐Bablok analyses were used to compare potassium concentrations generated by the VS2 and Cobas analyzers. Least squares regression analysis was performed to evaluate if a linear relationship exists between VS2 potassium concentrations and Cobas CK activities.ResultsPotassium concentrations from the VS2 were higher (median and standard deviation (SD) = 5.2 +/− 0.46 mmol/L) than those from the Cobas analyzer (4.4 +/− 0.35 mmol/L;P < 0.000), and a positive mean bias was found (mean difference = 0.774 mmol/L; 95% Confidence Interval (CI) = 0.706–0.842; limits of agreement = 0.21–1.34). Passing‐Bablok regression showed a positive proportional bias for potassium concentrations on the VS2 compared with paired Cobas results (Slope = 1.328; 95% CI = 1.100–1.500) but did not reveal systematic bias (Intercept = −0.714; 95% CI = −1.46–0.265). Least squares regression analysis showed a poor non‐significant relationship (R2 = 0.19) between potassium measured by the VS2 and CK measured by the Cobas analyzer.ConclusionsA defined threshold value of CK activity at which potassium concentration begins to falsely increase when measured on the VS2 was not established as data widely varied. A poor non‐significant relationship between potassium concentrations and CK activities did not allow prediction of the threshold at which falsely increased potassium concentrations would be expected on the VS2.
Olfactory neuroblastoma in a domestic cat and review of the literature
Authors: Martí‐García Bernat , Priestnall Simon L. , Holmes Emma , Suárez‐Bonnet Alejandro 
Published: July 2023

AbstractNasal tumors account for less than 10% of all feline neoplasms, with lymphoma, followed by adenocarcinoma, and squamous cell carcinoma, the most commonly reported. Nasal neuroectodermal tumors, including olfactory neuroblastoma (ONB), are scarcely described, and their tumorigenesis is largely unknown. Here we report the cytological, histological, and immunohistochemical features of a feline ONB. We also provide a pathological review of nasal neuroendocrine neoplasms in cats. A 7‐year‐old Burmese cat was evaluated for sneezing, occasional epistaxis, and upper respiratory noise for 8 months. Computed tomography (CT) imaging revealed a 7 × 5 × 3 mm irregular mass effacing and expanding the nasal cavity, which extended to the nasopharynx. Cytologically, neoplastic cells were round to polygonal and had a round nucleus with finely stippled chromatin, a single small nucleolus, and abundant pale blue cytoplasm, which contained abundant fine pale pink granules. They exhibited mild cellular atypia, anisocytosis, and mild to occasionally moderate anisokaryosis. Rhinoscopic biopsies revealed a densely cellular, malignant neuroepithelial neoplasm. Cells were arranged in densely packed trabeculae and formed Homer Wright and Flexner–Wintersteiner‐like rosettes, with rare mitotic figures and scant supportive fibrovascular stroma. Immunohistochemically, neoplastic cells were positive for vimentin, cytokeratin AE1/AE3, COX‐2, and beta‐tubulin and negative for S‐100, chromogranin A, CD117, and epithelial membrane antigen (EMA). An ONB was diagnosed based on histological and immunohistochemical findings. Interestingly, and similar to nasal carcinomas, neoplastic cells diffusely neo‐expressed COX‐2. To the authors′ knowledge, there is no previous evidence of COX‐2 in feline ONB. Histopathology and immunohistochemistry are required for a definitive diagnosis of ONB.
A mismatch of planning and achieved tibial plateau angle in cranial closing wedge surgery: An in silico and clinical evaluation of 100 cases
Authors: Banks Charlotte , Jones Gareth Michael Couper , Meeson Richard Lawrence 
Published: July 2023

AbstractObjective(1) To determine whether Oxley's modified cranial closing wedge osteotomy (CCWO) results in a tibial plateau angle (TPA) of 5° in silico, (2) compare in silico to clinical postoperative TPA and (3) determine the impact of ostectomy distalization in silico.Study designCross‐sectional retrospective radiographic in silico study.Sample populationA total of 100 stifle radiographs; 90 dogs (small ≤25 kg = 84; large >25 kg = 16).MethodsTibial plateau angles were measured preoperatively (TPAPre), after in silico planning (TPAPlan), and postoperatively (TPAPost). Virtual ostectomies were evaluated for plate fit. Postoperative ostectomy position was measured. Virtual ostectomies were performed 5/7.5/10/15 mm from the patellar tendon insertion in 10 dogs from each preoperative TPA category (≤20°/21–25°/ 26–30°/31–35°/>35°). Comparisons for TPAPre, TPAPost, and TPAPlan were made between small and large dogs, and the outcome to the target 5° between TPA categories and ostectomy positions.ResultsMean ± SD TPAPre was 28.6 ± 6.2° and higher in small than large dogs. Mean TPAPlan was 7.6 ± 2.7°. Plate fit was appropriate in all. In silico, TPAs were greater than 5° except for cases with TPAPre > 35°. Median TPAPost was 5.5° (−4–21) and was higher in small (7°) than large (4.5°) dogs. Postoperative ostectomy position was more distal than Oxley's guidelines. When distalized >7.5 mm in silico, the magnitude of under‐correction increased.ConclusionsOxley's modified CCWO did not result in TPA of 5° in most cases. Ostectomy distalization exacerbated under‐correction.Clinical impactCurrent guidelines should be modified to achieve 5° TPA postoperatively.
Chinese migrants’ experiences of responding to gambling harm in New Zealand
Authors: Zhang Wenli , Fouché Christa , Adams Peter James 
Published: July 2023

INTRODUCTION: Forming a problematic relationship with gambling has major consequences for gamblers, families, communities, and society. As the third-largest ethnic group in New Zealand, the Chinese community in particular faces increasing challenges with casino gambling. This paper reports on Chinese migrants’ lived experience of their challenges and needs in responding to gambling harm.
METHODS: Sixteen recent migrants (both gamblers and affected family members) from eight families were interviewed. Data analysis comprised a comprehensive thematic approach involving multiple readings of interview transcripts and an iterative development of themes, guided by hermeneutic phenomenological methods.
FINDINGS: Participants shared their experiences of pathways into gambling and ways to respond to gambling harm. The key findings are presented as four stages, which can be conceptualised as misconnecting, disconnecting, reconnecting, and rebuilding natural life.
CONCLUSION: This article presents qualitative evidence of Chinese migrants in New Zealand’s experiences with excessive gambling and considerations for service providers and policymakers when developing programmes and policies for preventing and minimising gambling harm for this population.
We need to talk about self-care (but not in the way you think)
Authors: Berkowitz Allison 
Published: July 2023

Self-care is widely acknowledged as crucial in the social work profession. While this area of research has begun to expand in recent decades, there is still much to be explored. Most studies in this field cover issues like the types and frequency of self-care engaged in, howto teach it, and how it relates to the prevention of issues like burnout. This research brief will review the literature surrounding these important matters, highlighting a gap pertaining to the practical understanding and application of self-care. How do social workers and social work students think and feel about self-care and the way it is currently taught? Do they know how often most of their peers engage in self-care? Do they know how to realistically incorporate self-care into their own lives? This research brief will discuss what is currently known about the answers to these questions, culminating in suggestions for future research and recommendations that will give future social workers realistic expectations and tools with which to enter the field.
Histone Methyltransferases as a New Target for Epigenetic Action of Vorinostat
Authors: Maksimova Varvara , Makus Julia , Popova Valeriia , Prus Anzhelika , Usalka Olga , Trapeznikova Ekaterina , Zhidkova Ekaterina , Belitsky Gennady , Yakubovskaya Marianna , Kirsanov Kirill 
Published: July 2023

Abstract
Epigenetic genome regulation during malignant cell transformation is characterized by the aberrant methylation and acetylation of histones. Vorinostat (SAHA) is an epigenetic modulator actively used in clinical oncology. The antitumor activity of vorinostat is commonly believed to be associated with the inhibition of histone deacetylases, while the impact of this drug on histone methylation has been poorly studied. Using HeLa TI cells as a test system allowing evaluation of the effect of epigenetically active compounds from the expression of the GFP reporter gene and gene knockdown by small interfering RNAs, we showed that vorinostat not only suppressed HDAC1, but also reduced the activity of EZH2, SUV39H1, SUV39H2, and SUV420H1. The ability of vorinostat to suppress expression of EZH2, SUV39H1/2, SUV420H1 was confirmed by Western blotting. Vorinostat also downregulated expression of SUV420H2 and DOT1L enzymes. The data obtained expand our understanding of the epigenetic effects of vorinostat and demonstrate the need for a large-scale analysis of its activity toward other enzymes involved in the epigenetic genome regulation. Elucidation of the mechanism underlying the epigenetic action of vorinostat will contribute to its more proper use in the treatment of tumors with an aberrant epigenetic profile.
Fifteen-minute consultation: How to approach the initial discussions of dysmorphism with parents
Authors: Parmenter Kate Frances , English Sharon , Campbell Jennifer 
Published: July 2023

Approaching discussions and introducing the idea of dysmorphology to parents and families can be perceived as difficult and even daunting by health professionals resulting in uneasiness in how best to initiate important dialogues sensitively and accessibly.Here, we offer a structured approach to the assessment of, and subsequent initial discussion around, dysmorphism and potential syndromic features in babies and children.
Precision measurement of the specific activity of $$^{39}$$Ar in atmospheric argon with the DEAP-3600 detector
Authors: Adhikari P. , Ajaj R. , Alpízar-Venegas M. , Amaudruz P.-A. , Anstey J. , Araujo G. R. , Auty D. J. , Baldwin M. , Batygov M. , Beltran B. , Benmansour H. , Bina C. E. , Bonatt J. , Bonivento W. , Boulay M. G. , Broerman B. , Bueno J. F. , Burghardt P. M. , Butcher A. , Cadeddu M. , Cai B. , Cárdenas-Montes M. , Cavuoti S. , Chen M. , Chen Y. , Choudhary S. , Cleveland B. T. , Corning J. M. , Crampton R. , Cranshaw D. , Daugherty S. , DelGobbo P. , Dering K. , Stefano P. Di , DiGioseffo J. , Dolganov G. , Doria L. , Duncan F. A. , Dunford M. , Ellingwood E. , Erlandson A. , Farahani S. S. , Fatemighomi N. , Fiorillo G. , Florian S. , Flower A. , Ford R. J. , Gagnon R. , Gallacher D. , Abia P. García , Garg S. , Giampa P. , Giménez-Alcázar A. , Goeldi D. , Golovko V. V. , Gorel P. , Graham K. , Grant D. R. , Grobov A. , Hallin A. L. , Hamstra M. , Harvey P. J. , Haskins S. , Hearns C. , Hu J. , Hucker J. , Hugues T. , Ilyasov A. , Jigmeddorj B. , Jillings C. J. , Joy A. , Kamaev O. , Kaur G. , Kemp A. , Kuźniak M. , Zia F. La , Lai M. , Langrock S. , Lehnert B. , Leonhardt A. , LePage-Bourbonnais J. , Levashko N. , Lidgard J. , Lindner T. , Lissia M. , Lock J. , Luzzi L. , Machulin I. , Majewski P. , Maru A. , Mason J. , McDonald A. B. , McElroy T. , McGinn T. , McLaughlin J. B. , Mehdiyev R. , Mielnichuk C. , Mirasola L. , Monroe J. , Nadeau P. , Nantais C. , Ng C. , Noble A. J. , O’Dwyer E. , Oliviéro G. , Ouellet C. , Pal S. , Papi D. , Pasuthip P. , Peeters S. J. M. , Perry M. , Pesudo V. , Picciau E. , Piro M.-C. , Pollmann T. R. , Rad F. , Rand E. T. , Rethmeier C. , Retière F. , Rodríguez García I. , Roszkowski L. , Ruhland J. B. , Santorelli R. , Schuckman II F. G. , Seeburn N. , Seth S. , Shalamova V. , Singhrao K. , Skensved P. , Smith N. J. T. , Smith B. , Sobotkiewich K. , Sonley T. , Sosiak J. , Soukup J. , Stainforth R. , Stone C. , Strickland V. , Stringer M. , Sur B. , Tang J. , Vázquez-Jáuregui E. , Veloce L. , Viel S. , Vyas B. , Walczak M. , Walding J. , Ward M. , Westerdale S. , Willis J. , Zuñiga-Reyes A. ,   DEAP Collaboration
Published: July 2023

AbstractThe specific activity of the $$\beta $$
β
decay of $$^{39}$$

39

Ar in atmospheric argon is measured using the DEAP-3600 detector. DEAP-3600, located 2 km underground at SNOLAB, uses a total of (3269 ± 24) kg of liquid argon distilled from the atmosphere to search for dark matter. This detector is well-suited to measure the decay of $$^{39}$$

39

Ar owing to its very low background levels. This is achieved in two ways: it uses low background construction materials; and it uses pulse-shape discrimination to differentiate between nuclear recoils and electron recoils. With 167 live-days of data, the measured specific activity at the time of atmospheric extraction is (0.964 ± 0.001$$_\textrm{stat}$$

stat

± 0.024$$_\textrm{sys}$$

sys

) Bq/kg$$_\textrm{atmAr}$$

atmAr

, which is consistent with results from other experiments. A cross-check analysis using different event selection criteria and a different statistical method confirms the result.
Danicamtiv Increases Myosin Recruitment and Alters Cross-Bridge Cycling in Cardiac Muscle
Authors: Kooiker Kristina B. , Mohran Saffie , Turner Kyrah L. , Ma Weikang , Martinson Amy , Flint Galina , Qi Lin , Gao Chengqian , Zheng Yahan , McMillen Timothy S. , Mandrycky Christian , Mahoney-Schaefer Max , Freeman Jeremy C. , Costales Arenas Elijah Gabriela , Tu An-Yu , Irving Thomas C. , Geeves Michael A. , Tanner Bertrand C.W. , Regnier Michael , Davis Jennifer , Moussavi-Harami Farid 
Published: July 2023

BACKGROUND:
Modulating myosin function is a novel therapeutic approach in patients with cardiomyopathy. Danicamtiv is a novel myosin activator with promising preclinical data that is currently in clinical trials. While it is known that danicamtiv increases force and cardiomyocyte contractility without affecting calcium levels, detailed mechanistic studies regarding its mode of action are lacking.

METHODS:
Permeabilized porcine cardiac tissue and myofibrils were used for X-ray diffraction and mechanical measurements. A mouse model of genetic dilated cardiomyopathy was used to evaluate the ability of danicamtiv to correct the contractile deficit.

RESULTS:
Danicamtiv increased force and calcium sensitivity via increasing the number of myosins in the ON state and slowing cross-bridge turnover. Our detailed analysis showed that inhibition of ADP release results in decreased cross-bridge turnover with cross bridges staying attached longer and prolonging myofibril relaxation. Danicamtiv corrected decreased calcium sensitivity in demembranated tissue, abnormal twitch magnitude and kinetics in intact cardiac tissue, and reduced ejection fraction in the whole organ.

CONCLUSIONS:
As demonstrated by the detailed studies of Danicamtiv, increasing myosin recruitment and altering cross-bridge cycling are 2 mechanisms to increase force and calcium sensitivity in cardiac muscle. Myosin activators such as Danicamtiv can treat the causative hypocontractile phenotype in genetic dilated cardiomyopathy.
Hypertensive Adults Exhibit Lower Myelin Content: A Multicomponent Relaxometry and Diffusion Magnetic Resonance Imaging Study
Authors: Laporte John P. , Faulkner Mary E. , Gong Zhaoyuan , Akhonda Mohammad A.B.S. , Ferrucci Luigi , Egan Josephine M. , Bouhrara Mustapha 
Published: July 2023

BACKGROUND:
It is unknown whether hypertension plays any role in cerebral myelination. To fill this knowledge gap, we studied 90 cognitively unimpaired adults, age range 40 to 94 years, who are participants in the Baltimore Longitudinal Study of Aging and the Genetic and Epigenetic Signatures of Translational Aging Laboratory Testing to look for potential associations between hypertension and cerebral myelin content across 14 white matter brain regions.

METHODS:

Myelin content was probed using our advanced multicomponent magnetic resonance relaxometry method of myelin water fraction, a direct and specific magnetic resonance imaging measure of myelin content, and longitudinal and transverse relaxation rates (
R

1

and
R

2

), 2 highly sensitive magnetic resonance imaging metrics of myelin content. We also applied diffusion tensor imaging magnetic resonance imaging to measure fractional anisotropy, mean diffusivity, radial diffusivity, and axial diffusivity values, which are metrics of cerebral microstructural tissue integrity, to provide context with previous magnetic resonance imaging findings.

RESULTS:

After adjustment of age, sex, systolic blood pressure, smoking status, diabetes status, and cholesterol level, our results indicated that participants with hypertension exhibited lower myelin water fraction, fractional anisotropy,
R

1

and
R

2

values and higher mean diffusivity, radial diffusivity, and axial diffusivity values, indicating lower myelin content and higher impairment to the brain microstructure. These associations were significant across several white matter regions, particularly in the corpus callosum, fronto-occipital fasciculus, temporal lobes, internal capsules, and corona radiata.

CONCLUSIONS:
These original findings suggest a direct association between myelin content and hypertension and form the basis for further investigations including longitudinal assessments of this relationship.
Addressing the Ocean-Climate Nexus in the BBNJ Agreement: Strategic Environmental Assessments, Human Rights and Equity in Ocean Science
Authors: Morgera Elisa , McQuaid Kirsty , La Bianca Giulia , Niner Holly , Shannon Lynne , Strand Mia , Rees Sian , Howell Kerry , Snow Bernadette , Lancaster Alana Malinde SN , Sauer Warwick 
Published: July 2023

Abstract
The Agreement on Marine Biodiversity of Areas beyond National Jurisdiction (BBNJ Agreement) opens a new path in international law towards addressing issues at the ocean-climate nexus, as well as considering implications for the protection of human rights and achieving equity among States in the context of ocean knowledge production and environmental management. Based on an interdisciplinary reflection, the new international obligations on strategic environmental assessments (SEAs), and new institutional arrangements, are identified as crucial avenues to addressing climate change mitigation and ensuring fair research partnerships, mutual capacity-building and technology co-development between the Global North and South. SEAs can also support integrated implementation of other parts of the BBNJ Agreement and contribute to the broader effectiveness of the general provisions of the United Nations Convention on the Law of the Sea on the protection of the marine environment, within and beyond national jurisdiction.