bluuebunny/crossref_metadata_2025_split huggingface dataset:
Revealing the Influence of Electrolyte Additives in Reducing the Heat Generation of High Voltage Lithium‐Ion Batteries using Operando Accelerating Rate Calorimetry (ARC)
Authors: Baazizi Mariam , Sayah Simon , Karbak Mehdi , Hajjaj Oumaima , Aqil Mohamed , Dahbi Mouad , Ghamouss Fouad 
Published: June 2023

AbstractLithium‐ion batteries operating at high voltage generally endure drastic capacity fading and serious safety issues. Working on the electrolytes’ stability can be a solution to mitigate these problems related to high voltage. Herein, the beneficial impact of functional electrolyte additives in a state‐of‐the‐art carbonate‐based electrolyte is demonstrated. The combination of fluoroethylene carbonate (FEC) with succinonitrile (SN) as additives was used to enhance the thermal stability of the electrolyte reference 1 M LiPF6 in EC:DMC (1 : 1, by weight) and cycling stability of a high voltage lithium‐ion device, consisting of a LiMn1.5Ni0.5O4 cathode and a metallic lithium anode. The electrolyte using the FEC/SN mixture displayed a wider electrochemical stability window (ESW) exhibited by linear sweep voltammetry (LSV). Furthermore, this electrolyte allowed the device to exhibit better rate capability and a capacity retention of 75 % after 100 cycles. Interestingly, the FEC+SN‐based electrolyte exhibited better thermal stability using operando accelerating rate calorimetry (ARC) by virtue of the lower heat quantity generated by the battery device. The remarkable improvements can be ascribed to the formation of a protective cathode‐electrolyte interface (CEI) produced by interfacial reactions between the cathode surface and electrolyte compounds.
Efficient secretion of mussel adhesion proteins using a chaperone protein Spy as fusion tag inBacillus subtilis
Authors: Wu Panpan , Tao Qing , Liu Yuxuan , Zeng Caiting , Li Yu , Yan Xin 
Published: June 2023

AbstractBackgroundMussel foot proteins (Mfps) are considered as remarkable materials due to their extraordinary adhesive capability. Recombinant expression is an ideal way to synthesis these proteins at large scale. However, secretory expression of Mfps into culture medium has not been achieved in a heterologous host.Methods and ResultsHere, to realize the secretion of Mfp3 and Mfp5 inBacillus subtilis, signal peptide screening was first performed. Minimal Mfp3‐6×His was targeted into the growth medium with AmyE signal peptide. We found that a small chaperone protein Spy was secreted efficiently inB. subtilis, and the fusion proteins Spy‐Mfp3‐6×His and Spy‐Mfp5‐6×His could also be delivered into growth medium well. The yield of Spy‐Mfp3‐6×His and Spy‐Mfp5‐6×His reached 255 and 119 mg L−1at shake flask conditions, respectively. Mfp3‐6×His and Mfp5‐6×His were finally purified via TEV protease cleavage and NTA affinity chromatography.ConclusionMfp3‐6×His and Mfp5‐6×His could be efficiently secreted using a chaperone protein Spy as fusion tag inB. subtilis.
A current view on the neglected topic of male child sexual abuse in the context of literature
Authors: Sari Seda Aybuke , Bilac Oznur , Erten Almak Tilbe , Butun Celal 
Published: June 2023

AbstractDespite the growing numbers, awareness and reporting rates of male child sexual abuse (MCSA) are still well below the real values, especially in developing countries like Turkey. We examined MCSA to determine the predisposing factors, the characteristics of perpetrators and victims, and the consequences of sexual abuse in a Turkish sample. We retrospectively analysed the files and forensic reports of 51 MCSA victims aged between 6 and 18. The mean age of children was 13.6 ± 3.8 years, and the mean age at the time of abuse was 11.1 ± 3.52. The average exposure time for sexual abuse was 91 ± 226.8 days, and the frequency was 2.4 ± 2.47 times. 17.6 per cent of them had previously been sexually abused. Disclosure times until legal notification was 240.1 ± 552.25 days. Most (56.9 per cent) had suffered anal penetration, developed post traumatic stress disorder, and needed medical treatment. Most perpetrators (58.8 per cent) were strangers outside the family, reaching the children from the neighbourhood. In conclusion, although the number of our sample is limited, the results of our study will contribute valuably to the literature, given that there are few studies on MCSA. Further studies with large samples evaluating MCSA in multidimensional ways are needed to raise awareness and protect vulnerable children.
Combined Metabolite Analysis and Network Pharmacology to Elucidate the Mechanisms of Therapeutic Effect of Melastoma dodecandrum Ellagitannins on Abnormal Uterine Bleeding
Authors: Lu Qiu‐Ping , Wu Miao‐Li , Li Hui‐Lin , Zhou Yu , Xian Ming‐Hua , Huang Wei‐Zhong , Piao Xiu‐Hong , Ge Yue‐Wei 
Published: June 2023

AbstractThe abnormal uterine bleeding (AUB) is complex and usually leads to severe anemia. Melastomadodecandrum (MD) is clinically used for the treatment of metrorrhagia bleeding. The MD ellagitannins (MD‐ETs) had been evidenced being effective at hemorrhage, and exerts biological activities upon their metabolites including ellagic acid and urolithins. In this study, the blood‐permeated metabolites from theMD‐ETs were analyzed using LC‐MS approach, and 19 metabolites including ellagic acid and urolithin A derivatives were identified. Furthermore, a network pharmacology analysis including the target prediction analysis, AUB target analysis, Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis were conducted to reveal the relationships between “metabolites‐targets‐pathways”, which was further verified by molecular docking analysis. The results showed that methyl ellagic acid, urolithin A and isourolithin A produced from MD‐ETs can be absorbed into the blood, and might act on the core targets of VEGFA, SRC, MTOR, EGFR and CCND1. And the hemostatic effects were exerted through PI3K‐Akt, endocrine resistance and Rap 1 signaling pathways. These results implied the potential effective constituents and action mechanism of MD‐ETs in the therapy of AUB, which will promote the application of MD‐ETs as natural agent for the treatment of gynecological bleeding diseases.
Genome Mining and Molecular Networking Guided Isolation of Antimycin Analogs with Antifeedant Activities from the Deep‐Sea‐Derived Streptomyces sp. NA13
Authors: Bai Yan , Ren Duo , Li Fenglin , Hu Jiangchun , Pan Huaqi 
Published: June 2023

AbstractPolyphagous insects could affect agricultural production, which leads to serious economic losses. Due to the negative effects of synthesized insecticides, finding eco‐friendly and new biopesticides is emergent. To develop natural origin insecticides, an integrative approach combining antifeedant activity screening, genome mining, and molecular networking has been applied to discover antifeedant secondary metabolites from Streptomyces sp. NA13, which leads to the isolation of a novel antimycin Q (1) and six known antimycin analogs (antimycins A1a, A2a, A3a, A4a, A7a, and N‐formylantimycic acid methyl ester, 2–7). Their structures were identified by high‐resolution mass spectrometry (HR‐MS) and nuclear magnetic resonance (NMR) spectroscopic. The absolute configuration of 1 was elucidated by the comparison of coupling constant, electronic circular dichroism (ECD) analysis, and NMR calculations. 1–6 exhibited different levels of antifeedant activities against Helicoverpa armigera, especially 1–4. At the same time, the antifeedant activity of antimycin was reported firstly.
The s‐p Nonhybrid Nature Causes Adaptive Superatomic States of Bismuth Clusters
Authors: Geng Lijun , Du Qiuying , Li Mengxu , Yin Baoqi , Luo Zhixun , Zhao Jijun 
Published: June 2023

AbstractWe report a joint experimental and theoretical study on the stability and reactivity of Bin+ (n=5–33) clusters. The alternating odd‐even effect on the reaction rates of Bin+ clusters with NO is observed, and Bi7+ finds the most inertness. First‐principles calculation results reveal that the lowest energy structures of Bi6‐9+ exhibit quasi‐spherical geometry pertaining to the jellium shell model; however, the Bin+ (n≥10) clusters adopt assembly structures. The prominent stability of Bi7+ is associated with its highly symmetric structure and superatomic states with a magic number of 34e closed shell. For the first time, we demonstrate that the unique s‐p nonhybrid feature in bismuth rationalizes the stability of Bi6‐9+ clusters within the jellium model, by filling the 6s electrons into the superatomic orbitals (forming “s‐band”). Interestingly, the stability of 18e “s‐band” coincides with the compact structure for Bin+ at n≤9 but assembly structures for n≥10, showing an accommodation of the s electrons to the geometric structure. The atomic p‐orbitals also allow to form superatomic orbitals at higher energy levels, contributing to the preferable structures of tridentate binding units. We illustrate the s‐p nonhybrid nature accommodates the structure and superatomic states of bismuth clusters.
Evolving spectrum of arrhythmogenic cardiomyopathy: Implications for Sports Cardiology
Authors: Graziano Francesca , Cipriani Alberto , Balla Dorottya , Bondarev Sergei , Marra Martina Perazzolo , Bauce Barbara , Vágó Hajnalka , Corrado Domenico , Zorzi Alessandro 
Published: June 2023

AbstractArrhythmogenic cardiomyopathy (ACM) is a genetic heart muscle disease, structurally characterized by progressive fibro‐fatty replacement of the normal myocardium and clinically by ventricular arrhythmias (VAs). Predominantly thanks to the use of cardiac magnetic resonance, we have learnt that the spectrum of the disease encompasses not only the classical right ventricular phenotype, but also biventricular and left dominant variants. Sport activity contributes to the phenotypic expression and progression of ACM and may trigger life‐threatening VAs and sudden cardiac death (SCD). We conducted a review of the literature about ACM and its implications in Sport Cardiology and summarized the main findings in this topic. Early identification of affected athletes through preparticipation screening (PPS) is fundamental but, while classical right‐ventricular or biventricular phenotypes are usually suspected because of electrocardiogram (ECG) and echocardiographic abnormalities, variants with predominant left ventricular involvement are often characterized by normal ECG and unremarkable echocardiography. Usually the only manifestations of such variants are exercise‐induced VAs and for this reason exercise testing may empower the diagnostic yield of the PPS. Patients with ACM are not eligible to competitive sports activity, but low‐to‐moderate intensity physical activity under medical supervision is possible in most cases.
Comparative features of calretinin, calbindin, and parvalbumin expressing interneurons in mouse and monkey primary visual and frontal cortices
Authors: Medalla Maria , Mo Bingxin , Nasar Rakin , Zhou Yuxin , Park Junwoo , Luebke Jennifer I. 
Published: June 2023

AbstractFundamental differences in excitatory pyramidal cells across cortical areas and species highlight the implausibility of extrapolation from mouse to primate neurons and cortical networks. Far less is known about comparative regional and species‐specific features of neurochemically distinct cortical inhibitory interneurons. Here, we quantified the density, laminar distribution, and somatodendritic morphology of inhibitory interneurons expressing one or more of the calcium‐binding proteins (CaBPs) (calretinin [CR], calbindin [CB], and/or parvalbumin [PV]) in mouse (Mus musculus) versus rhesus monkey (Macaca mulatta) in two functionally and cytoarchitectonically distinct regions—the primary visual and frontal cortical areas—using immunofluorescent multilabeling, stereological counting, and 3D reconstructions. There were significantly higher densities of CB+ and PV+ neurons in visual compared to frontal areas in both species. The main species difference was the significantly greater density and proportion of CR+ interneurons and lower extent of CaBP coexpression in monkey compared to mouse cortices. Cluster analyses revealed that the somatodendritic morphology of layer 2–3 inhibitory interneurons is more dependent on CaBP expression than on species and area. Only modest effects of species were observed for CB+ and PV+ interneuron morphologies, while CR+ neurons showed no difference. By contrast to pyramidal cells that show highly distinctive area‐ and species‐specific features, here we found more subtle differences in the distribution and features of interneurons across areas and species. These data yield insight into how nuanced differences in the population organization and properties of neurons may underlie specializations in cortical regions to confer species‐ and area‐specific functional capacities.
Biomimetic Synthesis and Mechanism of Hydroxyapatite Crystals by Biotemplate of Small Intestinal Submucosa and Bacterial Cellulose
Authors: Zhu Qiuhong , Jiao Hua , Zhao Kang , Gou Xingchun , Tang Yufei , Ji Jingwen 
Published: June 2023

AbstractHydroxyapatite (HAp) is coassembled with many organic molecular templates led by collagens in natural bone. In this study, it is presumed that small intestinal submucosa (SIS) with acetamide group chelates calcium ion and bacterial cellulose (BC) has strong intermolecular hydroxyl group and powerful attraction for calcium ion to initiate initial nucleation. Phosphate ions bind calcium ions to form seed crystal of calcium phosphate, which is further directionally grown under the mediation of the template structure and finally forms weakly crystalline HAp, providing an environment for the HAp formation. Besides, the samples are characterized by X‐ray diffraction, Fourier transform infrared, X‐ray photoelectron spectroscopy, scanning electron microscope, and transmission electron microscope to unveil the phase structure, composition, and morphology, and a growth mechanism is proposed. HAp is successfully synthesized on SIS–BC biomineralization template. The results show morphology of samples from sheet‐lamellar‐like to flower‐like or porous‐spheroid‐like from 1 day to 7 days. The plate‐like HAp rods are polycrystal with c‐axis orientation. Interestingly, the HAp/SIS‐BC composites have the least relative mass of HAp on mineralization and the content of HAp (57%) is very close to the human bone tissue in structure (about 60%). Finally, the HAp mineralized films show good biocompatibility through MC3T3‐E1cells test for the potential biomaterials.
Influence of skin type and laser wavelength on laser‐evoked potentials
Authors: Frahm Ken Steffen , Gervasio Sabata , Arguissain Federico , Mouraux André 
Published: June 2023

AbstractBackgroundInfrared laser stimulation is a valuable tool in pain research, its primary application being the recording of laser‐evoked brain potentials (LEPs). Different types of laser stimulators, varying in their skin penetrance, are likely to have a large influence on the LEPs, when stimulating different skin types. The aim of this study was to investigate how LEPs depend on laser type and skin location.MethodsTwo different laser stimulators (CO2 and Nd:YAP) were used to compare LEPs in healthy subjects. Stimuli were delivered to the hand dorsum and palm to investigate the effects of skin type on the evoked responses. Stimulus‐evoked brain responses were recorded using EEG and perceived intensity ratings were recorded. Computational modelling was used to investigate the observed differences.ResultsLEPs evoked by stimulation of the hairy skin were similar between CO2 and Nd:YAP stimulation. In contrast, LEPs elicited from the palm were markedly different and barely present for CO2 stimulation. There was a significant interaction between laser type and skin type (RM‐ANOVA, p < 0.05) likely due to smaller CO2 LEPs in the palm. CO2 stimuli to the palm also elicited significantly lower perceived intensities. The computational model showed that the observed differences were explainable by the laser absorption characteristics and skin thickness affecting the temperature profile at the dermo‐epidermal junction (DEJ).ConclusionsThis study shows that LEP elicitation depends on the combination of laser penetrance and skin type. Low penetrance stimuli, from a CO2 laser, elicited significantly lower LEPs and perceived intensities in the palm.SignificanceThis study showed that the elicitation of laser‐evoked potentials in healthy humans greatly depends on the combination of laser stimulator type and skin type. It was shown that high penetrance laser stimuli are capable of eliciting responses in both hairy and glabrous skin, whereas low penetrance stimuli barely elicited responses from the glabrous skin. Computational modelling was used to demonstrate that the results could be fully explained by the combination of laser type and skin thickness.
Research and scholarly methods: Studies of workplace well‐being and other organizational phenomena
Authors: Reed Brent N. , McGonagle Alyssa K. 
Published: June 2023

AbstractAs research in pharmacy increasingly focuses on work‐related topics such as burnout, workforce management, and diversity, equity, and inclusion, there is a growing need for scholars to understand and use organizational research methods. However, these skills are not typically taught in pharmacy schools or postgraduate training programs, posing a challenge to researchers and peer reviewers alike. To address this gap, we aim to provide a practical introduction to organizational research methods with a focus on deductive, quantitative approaches. Given the growing interest in studying pharmacists' workplace well‐being, we use this as a theme for our review. We begin by discussing the importance of a thorough literature review and using theory to frame the research, develop study hypotheses, and select an appropriate research design. We also provide a list of example theories that researchers could use to study the topic of workplace well‐being. Next, we discuss key considerations for defining and measuring organizational constructs, including validity and reliability, and highlight potential pitfalls to avoid in survey research. Using several phenomena of interest in studies of workplace well‐being, we provide examples of construct definitions and illustrate how they could be operationalized as variables in a research study. Finally, we discuss important considerations for analyzing organizational data and describe skills that researchers should acquire or seek in potential collaborators. To aid researchers and peer reviewers, we provide a checklist summarizing our recommendations and references to additional resources. By equipping scholars with a better understanding of organizational research methods, we aim to enhance our understanding of pharmacists' work experiences and generate evidence to support workplace improvements that will benefit pharmacists and their efforts to provide patient care.
Nanoscale implant anchorage aided by cement line deposition into titanium dioxide nanotubes
Authors: Grandfield Kathryn , Binkley Dakota Marie , Ay Birol , Liu Zhen Mei , Wang Xiaoyue , Davies John E. 
Published: June 2023

AbstractThe success of titanium dental implants relies on osseointegration, the load‐bearing connection between bone tissue and the device that, in contact osteogenesis, comprises the deposition of bony cement line matrix onto the implant surface. Titanium dioxide nanotubes (NTs) are considered a promising surface for improved osseointegration, yet the mechanisms of cement line integration with such features remains elusive. Herein, we illustrate cement line deposition into NTs on the surface of titanium implants with two underlaying microstructures: a machined surface or a blasted/acid etched surface placed in the tibiae of Wistar rats. After retrieval, scanning electron microscopy of tissue reflected from the implant surface indicated minimal incursion of the cement line matrix into the NTs. To investigate this further, focused ion beam was utilized to prepare cross‐sectional samples that could be characterized using scanning transmission electron microscopy. The cement line matrix covered NTs regardless of underlying microstructure, which was further confirmed by elemental analysis. In some instances, cement line infiltration into the NTs was noted, which reveals a mechanism of nanoscale anchorage. This study is the first to demonstrate cement line deposition into titanium NTs, suggesting nano‐anchorage as a mechanism for the success of the NT modified surfaces in vivo.
Exploring the inhibition mechanism of SARS‐CoV‐2 main protease by ebselen: A molecular docking, molecular dynamics simulation and DFT approach
Authors: You Wanli , Chen Yuandao 
Published: June 2023

AbstractThe main protease (Mpro) of SARS‐CoV‐2 plays an essential role in the virus life cycle and is considered a key target for therapeutic development. This study explores the inhibition mechanism of SARS‐CoV‐2 Mpro by ebselen, an organoselenium drug that shows potent inhibitory activity. By using a combination of multiple computational methods including molecular docking, molecular dynamics simulations, and density functional theory calculations, the complete covalent inhibition process of ebselen is simulated for the first time. Two possible pathways with different bound conformations of ebselen are identified. The hydrolysis of the enzyme‐ebselen adduct is found to be the rate‐determining step. The simulation results show that the behavior of water molecules at the hydrolysis site is crucial to distinguish the two paths energetically. Our simulations, which are in agreement with existing experimental results, provide a theoretical basis for the rational design and mechanism exploration of ebselen‐based inhibitors.
Periodontal ligament‐associated protein‐1 knockout mice regulate the differentiation of osteoclasts and osteoblasts through TGF‐β1/Smad signaling pathway
Authors: Liu Shuang , Yan Xiao , Guo Jing , An Hong , Li Xingrui , Yang Liying , Yu Xijiao , Li Shu 
Published: June 2023

AbstractIt has been known that periodontal ligament‐associated protein‐1 (PLAP‐1/Asporin) not only inhibits cartilage formation in osteoarthritis, but it also influences the healing of skull defect. However, the effect and mechanism of PLAP‐1/Asporin on the mutual regulation of osteoclasts and osteoblasts in periodontitis are not clear. In this study, we utilized a PLAP‐1/Asporin gene knockout (KO) mouse model to research this unknown issue. We cultured mouse bone marrow mesenchymal stem cells with Porphyromonas gingivalis lipopolysaccharide (P.g. LPS) for osteogenic induction in vitro. The molecular mechanism of PLAP‐1/Asporin in the regulation of osteoblasts was detected by immunoprecipitation, immunofluorescence, and inhibitors of signaling pathways. The results showed that the KO of PLAP‐1/Asporin promoted osteogenic differentiation through transforming growth factor beta 1 (TGF‐β1)/Smad3 in inflammatory environments. We further found the KO of PLAP‐1/Asporin inhibited osteoclast differentiation and promoted osteogenic differentiation through the TGF‐β1/Smad signaling pathway in an inflammatory coculture system. The experimental periodontitis model was established by silk ligation and the alveolar bone formation in PLAP‐1/Asporin KO mice was promoted through TGF‐β1/Smad3 signaling pathway. The subcutaneous osteogenesis model in nude mice also confirmed that the KO of PLAP‐1/Asporin promoted bone formation by the histochemical staining. In conclusion, PLAP‐1/Asporin regulated the differentiation of osteoclasts and osteoblasts through TGF‐β1/Smad signaling pathway. The results of this study lay a theoretical foundation for the further study of the pathological mechanism underlying alveolar bone resorption, and the prevention and treatment of periodontitis.