bluuebunny/crossref_metadata_2025_split huggingface dataset:
Machine learning models to complete rainfall time series databases affected by missing or anomalous data
Authors: Lupi Andrea , Luppichini Marco , Barsanti Michele , Bini Monica , Giannecchini Roberto 
Published: July 2023

Abstract
In recent years, artificial intelligence in geosciences is spreading more and more, thanks to the availability of a large amount of data. In particular, the development of automatic raingauges networks allows to get rainfall data and makes these techniques effective, even if the performance of artificial intelligence models is a consequence of the coherency and quality of the input data. In this work, we intended to provide machine learning models capable of predicting rainfall data starting from the values of the nearest raingauges at one historic time point. Moreover, we investigated the influence of the anomalous input data on the prediction of rainfall data. We pursued these goals by applying machine learning models based on Linear Regression, LSTM and CNN architectures to several raingauges in Tuscany (central Italy). More than 75% of the cases show an R² higher than 0.65 and a MAE lower than 4 mm. As expected, we emphasized a strong influence of the input data on the prediction capacity of the models. We quantified the model inaccuracy using the Pearson's correlation. Measurement anomalies in time series cause major errors in deep learning models. These anomalous data may be due to several factors such as temporary malfunctions of raingauges or weather conditions. We showed that, in both cases, the data-driven model features could highlight these situations, allowing a better management of the raingauges network and rainfall databases.
Identification and expression analysis of an IL-21 receptor in large yellow croaker (Larimichthys crocea)
Authors: Wu Hanyu , Fu Qiuling , Teng Yan , Mu Pengfei , Chen Jingjie , Chen Xinhua 
Published: July 2023

Abstract
Interleukin-21 (IL-21) is a potent immunomodulatory cytokine with pleiotropic effects on both innate and adaptive immune responses. It signaling involves a heterodimeric receptor complex consisting of the IL-21 receptor (IL-21R) and the common γ chain (γC). In this study, we identified a homologue gene of IL-21R (LcIL-21R) in the large yellow croaker (Larimichthys crocea, Lc). The open reading frame (ORF) of LcIL-21R gene is 1629 bp long and encodes a precursor protein of 542 amino acids (aa), with a 23-aa signal peptide and a 519-aa mature peptide containing four putative N-glycosylation sites. LcIL-21R has two fibronectin type III (FNIII)-like domains (D1 and D2), a transmembrane domain, and a cytoplasmic region. A conserved WSXWS motif was also found in the D2 domain. The predicted structure of the extracellular region of LcIL-21R (LcIL-21R-Ex) is highly similar to that of human IL-21R. LcIL-21R was constitutively expressed in all tissues examined, and LcIL-21R mRNA levels were increased in head kidney and spleen upon inactivated trivalent bacterial vaccine or poly(I:C) stimulation. Overall, our results suggest that LcIL-21R shares structural and functional properties with IL-21Rs found in other vertebrates, indicating its potential involvement in the IL-21-mediated immune response to pathogenic infections. These findings contribute to our understanding of the evolutionary conservation of IL-21 signaling and its role in the immune system.
99mTc(CO)3-labeled 1-(2-Pyridyl)piperazine derivatives as radioligands for 5HT7 receptors
Authors: Mardanshahi Alireza , Vaseghi Samaneh , Hosseinimehr Seyed Jalal , Abedi Seyed Mohammad , Molavipordanjani Sajjad 
Published: July 2023

Abstract
The 5-hydroxytryptamine receptor (5-HTR) family involves seven classes of receptors. The 5-HT7R is the newest member of this family and contributes to different physiological and pathological processes. As a pathology, Glioblastoma multiform (GBM) overexpresses 5-HT7R; hence, this study aims to develop radiolabeled aryl piperazine derivatives as 5-HT7R imaging agents. To that end, compounds 6 and 7 as 1-(3-nitropyridin-2-yl)piperazine derivatives were radiolabeled with fac-[99mTc(CO)3(H2O)3]+ and (99mTc(CO)3-[6] and 99mTc(CO)3-[7]) were obtained with high radiochemical purity (RCP>94%). 99mTc(CO)3-[6] and 99mTc(CO)3-[7] possess high affinity toward 5-HT7R of the U-87 MG cell line. The calculated Ki for them was 26.85±0.78 nM and 36.67±0.89 nM. The biodistribution study in normal mice indicates that the brain uptake of 99mTc(CO)3-[6] and 99mTc(CO)3-[7] is the highest at 30 min post-injection (0.8±0.25 and 0.64±0.18 %ID/g, respectively). The data of the biodistribution study in the U87-MG xenograft model revealed that these radiotracers could accumulate in the tumor site, and the highest tumor uptake was observed at 60 min post-injection (3.38±0.65 and 3.27±0.5 %ID/g, respectively). The injection of pimozide can block the tumor’s radiotracer uptake, indicating the binding of these radiotracers to the 5-HT7R. The imaging study in the xenograft model also confirms the biodistribution data. The acquired images clearly show the tumor site, and the tumor-to-muscle ratio for 99mTc(CO)3-[6] and 99mTc(CO)3-[7] at 60 min was 3.33 and 3.88, respectively.
Nigrostriatal inflammation is associated with nonmotor symptoms in an experimental model of prodromal Parkinson’s disease
Authors: Oliveira Bruna Silva , Toscano Eliana Cristina Brito , Abreu Larissa Katharina Sabino , Fernandes Heliana Barros , Amorim Renan Florindo , Ferreira Rodrigo Novaes , Machado Caroline Amaral , Carvalho Brener Cunha , Silva Maria Carolina Machado , Oliveira Antônio Carlos Pinheiro , Rachid Milene Alvarenga , Rocha Natália Pessoa , Teixeira Antônio Lúcio , Silva Elizabeth Ribeiro , Miranda Aline Silva 
Published: July 2023

Abstract

Recent evidence has supported a pathogenic role for neuroinflammation in Parkinson’s disease (PD). However, it is unclear whether the immune changes are involved in the initial physiopathology of PD, leading to the non-motor symptoms (NMS) observed in the prodromal PD stage. The current study aimed to characterize the behavioral and cognitive changes in a toxic-induced model of prodromal PD-like syndrome. We also sought to investigate the role of neuroinflammation in prodromal PD-related NMS. Male mice were subjected to bilateral intranasal (i.n.) infusion with 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) or saline (control group), followed by comprehensive behavioral and neurochemical analysis. Intranasal MPTP infusion was able to cause the loss of dopaminergic neurons. In parallel, it induced impairment in olfactory discrimination and social memory consolidation, compulsive and anxious-like behaviors, but did not influence motor function. In addition, iba-1 and GFAP expressions were increased in the SNpc, suggesting an activated state of microglia and astrocytes. Consistent with this finding, MPTP mice had increased levels of IL-10 and IL-17A, and decreased levels of BDNF and tropomyosin receptor kinase (Trk) A mRNA in the SNpc. The striatum showed increased IL-17A and decreased BDNF and NFG levels compared to control mice. In conclusion, our results suggest that neuroinflammation may play an important role in the early stage of experimental PD-like syndrome. Our data also indicate that i.n. administration of MPTP may represents a valuable mouse model for prodromal PD.
Cardiopulmonary baroreceptors modify pain intensity in patients with chronic back pain
Authors: Iwakuma Yuto , Clonch Davina A. , Liu Jennifer , Lam Christopher M. , Holwerda Seth 
Published: July 2023

Abstract

Objective Baroreceptors play a significant role in nociceptive pain. However, the extent to which baroreceptors modulate nociception in patients with chronic pain is unclear. We tested the hypothesis that cardiopulmonary baroreceptor unloading via LBNP would significantly increase pressure pain threshold and habituation to heat pain among patients with chronic back pain. Methods Mechanical pressure pain threshold at the upper trapezius (hand-held algometer) and habituation to heat pain at the forearm were performed during sitting and supine position, and during baroreceptor unloading via lower body negative pressure (LBNP) of -10 mmHg in 12 patients with chronic back pain (54 ± 11 years of age). To determine whether pain reduction is normal during LBNP, studies were repeated in 7 young, healthy participants (23 ± 7). Results Mechanical pressure pain threshold (P < 0.01) and habituation to heat pain (P = 0.04) were significantly reduced during supine compared with sitting. Conversely, baroreceptor unloading via LBNP significantly increased pressure pain threshold (P = 0.03) and heat pain habituation (P < 0.01) compared with supine. In young healthy controls, pressure pain threshold was similarly affected when comparing sitting and supine (P = 0.01) and during LBNP (P < 0.01), whereas habituation to heat pain was unaltered when comparing sitting and supine (P = 0.93) and during LBNP (P = 0.90). Total peripheral resistance was increased during LBNP (P = 0.01) but not among young, healthy controls (P = 0.71). Conclusions The findings demonstrate cardiopulmonary baroreceptor modulation of nociceptive pain in patients with chronic pain. Interestingly, habituation to heat pain appears more readily modified by cardiopulmonary baroreceptors in patients with chronic back pain compared with young, healthy individuals.
Oyster mushroom with short cropping duration and good taste developed through mono-monokaryon hybridization
Authors: Sindhu S , Theradimani M. , Vellaikumar S. , Paramasivam M. , Ramamoorthy V. 
Published: July 2023

Abstract
The development of short cropping duration oyster mushroom having good taste is one of the important needs of mushroom growers. Several Pleurotus spp. are long cropping duration in nature. Recently, we obtained Pleurotus djamor strain woody1 exhibiting a short cropping duration of 30 days and cultivated throughout the year, but it has a moderately acceptable taste. One of the cultivated Pleurotus spp. such as P. djamor strain MDU1 has good taste and is cultivated throughout the year but is a long cropping duration strain. Thus, genetic improvement of P. djamor was carried out by hybridization between these two strains by anastomosis. Monokaryons of parental strains showed variation in time required for germination – i.e. basidiospores of P. djamor strain woody1 germinated and developed monokaryotic colonies in six days, whereas that of P. djamor strain MDU1 developed monokaryotic colonies in eight days of incubation. In addition, variation in the growth rate and morphology of the monokaryotic mycelia of both parental strains was noticed. Out of 60 crosses made between mycelia of monokaryotic isolates of both parental strains, 20 crosses showed clamp connection, indicating that they were successful crosses. Out of 20 hybrids, two hybrid strains, viz., W2M4 and W4M4, exhibited higher yields than their parents. They both gained the short cropping duration trait, good taste attribute with good organoleptic values and some specific volatile metabolites. This study showed that anastomosis of two strains of P. djamor having desirable agronomic traits can be bred to develop an elite hybrid strain combining desirable agronomic traits.
A novel method for calculating interpolation points of NURBS curves based on chord length-parameter ratio
Authors: Hu Yifei , Jiang Xin , Huo Guanying , Su Cheng , Li Hexiong , Zheng Zhiming 
Published: July 2023

Abstract
Non-uniform rational B-spline (NURBS) curve has been widely used in computer numerical control machining due to its superior properties, such as global smoothness and local support. However, because of the nonanalytic relationship between the spline parameter and the arc length, it can be challenging to obtain accurate parameters when the arc length given. Approximation methods are commonly used because of their simplicity, but they can result in feedrate fluctuations that are difficult to avoid. This paper presents a feedback method to reduce feedrate fluctuations while maintaining simplicity. To obtain the initial parameter value of the next interpolation point, the chord length-parameter ratio (CPR) is defined first, and Newton's divided difference interpolation polynomial is applied to predict the CPR of the current interpolation point based on the CPRs of the historical interpolation points. Then, the initial parameter is corrected using the Second-order Runge-Kutta method with the compensation length, which is derived according to the tangent and chord directions at the initial parameter. The proposed method only requires two calculations of the first derivative of the NURBS curve, which is comparable to the computational complexity of most approximation methods. Numerical simulations are conducted to compare the performance of the proposed method with several approximation methods and a recent feedback method. The results demonstrate that the proposed method performs exceptionally well in terms of both efficiency and accuracy.
International Digital Technology Cycles and Local Economic Performance: The Balance between Concentration and Diversification
Authors: Woerter Martin , Heinrich Sebastian , Sarferaz Samad 
Published: July 2023

Abstract
The paper studies international dynamics in the development of digital technologies and their effect on local economic performance. We use a structural dynamic factor model to decompose trends in patenting activities across digital technology fields and countries into global and country-specific components. We then examine the impact of these global and country-specific digital technology development cycles on local economic performance and technological specialisation by regressing value added, employment, wages, labour productivity and digital technology specialisa-tion on world and country factors. The empirical results show that the global digital technology cycle plays a significant role in all countries. The study finds a positive relationship between the global digital technology cycle and the economic performance of a country’s ICT sectors. It shows that countries benefit from international efforts to develop digital technologies if their ICT sector encompasses a broad range of technologies and is not specialised in specific digital technologies
JEL classification: O31, O33, O47, C38, L86
A novel signature based on crosstalk between anoikis- and pyroptosis-associated genes for prediction of clinical outcomes, TME and treatment response of breast cancer
Authors: Liu Qian , Qu Fei , Wu Xuefang , Lu Rongrong , Huang Xiang , Yin Yongmei , Li Wei 
Published: July 2023

AbstractBackground:Breast cancer is nowthe most prevalent malignant among female population worldwide. Anoikis is a key progress during genesis and metastasis of malignant cells. Pyroptosis is a newly defined type of programmed cell death reported to have a dual effect on the development of carcinomas and had been reported to have the potential to affect anti-tumor immunity. However, few studies investigated the connections between anoikis, pyroptosis and prognosis in breast cancer.Methods:Anoikis and pyroptosis-related genes (APGs) were achieved from GeneCards and Harmonizome portals database. Based on expression profiles of APGs of patients from TCGA-BRCA cohort, differentiated expressed APGs between normal and tumoral tissues are identified. Next, by univariate Cox regression analysis of combined data of TCGA and GSE cohorts, prognostic APGs was defined. Then patients from both TCGA and GEO cohort were classified into three clusters by consensus clustering algorithm. Overlapped APGs between three clusters were identified as intersecting genes, based on expression of which, individuals are again assigned to two different gene clusters. Eventually, we successfully developed a PCA scoring signature and a nomogram system to accurately predict the prognosis and immunotherapy efficacy of breast cancer patients.Results:Patients were classified into three clusters based on APGs’ expression. Cluster A was featured by longest OS. According to the expression profile of 300 intersecting genes, patients were again divided into two different gene clusters. Subtype B is characterized with poorer diagnosis. Meanwhile, by means of principal component analysis, we successfully predicted clinical outcomes and treatment response to immunotherapy. Finally, we constructed an APG score-associated nomogram model to predict prognosis.Conclusion:We successfully established a scoring system based on anoikis and pyroptosis-related genes, as well as combined with clinicopathological features, to serve as a biomarker for prediction of clinical outcomes and immunotherapy efficacy in breast cancer.
Biosynthesis of apigenin glucosides in engineered Corynebacterium glutamicum
Authors: Amoah Obed Jackson , Ma Su Yeong , Thapa Samir Bahadur , Nguyen Hue Thi , Zaka Morshed Md , Sohng Jae Kyung 
Published: July 2023

Abstract

Background;
Glucosylation is a well-known approach to improve the solubility, pharmacological and biological properties of flavonoids. In recent years, efforts such as enzymatic synthesis have been developed to enhance the production of flavonoid glucosides. However, the low yield of products coupled with the requirement of expensive UDP-sugars limits the application of these systems for large-scale synthesis for human needs. C. glutamicum is a Gram-positive and generally regarded as safe (GRAS) bacteria frequently employed for the large-scale production of amino acids and bio-fuels. Due to the versatility of its cell factory system and its non-endotoxin producing properties, it has become an attractive system for the industrial-scale biosynthesis of alternate products. Here, we explored the cell factory of C. glutamicum for efficient glucosylation of flavonoids using apigenin as a model flavonoid.
Results;
For the production of apigenin glucosides, a promiscuous glycosyltransferase, YdhE from Bacillus licheniformis was successfully expressed in C. glutamicum. Additionally, the endogenous C. glutamicum genes galU1 encoding UDP-glucose pyrophosphorylase and pgm encoding phosphoglucomutase genes involved in the synthesis of UDP-glucose were overexpressed to create a C. glutamicum cell factory system capable of efficiently glucosylating apigenin with a high yield of glucosides in a comparatively short time. Consequently, the production of various apigenin glucosides was controlled under different temperatures yielding almost 4.2 mM of APG1(apigenin 4’-O-β-glucoside), 0.6 mM of APG2 (apigenin-7-O-β-glucoside), 1.7 mM of APG3 (apigenin 4’,7-O-β-diglucoside) and 2.1 mM of APG4 (apigenin 4’,5-O-β-diglucoside) after 40 h of incubation with the supplementation of 5 mM of apigenin.
Conclusion;
The developed C. glutamicum cell factory system highly glucosylated apigenin with higher efficiency and the high substrate susceptibility of C. glutamicum makes it the best alternative for large-scale biosynthesis of flavonoid glucosides. The developed system could be used to modify a wide range of plant secondary metabolites with increased pharmacokinetic activities on a large scale without the use of expensive UDP-sugars, thus making a cost-effective system.
Fabrication of Folic acid-cysteamine modified AgNPs as promising contrast agent for CT imaging
Authors: Lian Wei , Gan Min 
Published: July 2023

Abstract
The present work demonstrates the biosynthesis of silver nanoparticles (AgNPs) using Coffea arabica (C. arabica) leaf extract. The AgNPs prepared by green route from C. arabica leaf were characterized through UV-Visible, X-ray diffraction (XRD), Transmission electron microscopy (TEM), and Energy dispersive electron spectroscopy (EDS). Later, the prepared NPs were conjugated with cysteamine-folic acid and utilized as a contrast medium for in vitro targeted imaging of folic acid receptor-expressing malignant cells by computerized tomography (CT). At 80 kVp, the targeted cells exhibited CT values which were two times greater than that of the non-targeted cells. The results were compared with the folic acid-negative cell lines as well as the effective inhibition of folic acid receptor using free folic acid substrate. The outcome of the study suggests that the fabricated cysteamine-folic acid conjugated silver nanoparticles (FA-Cys-AgNPs) could be utilized as a potential contrast agent for molecular CT imaging. This information can be taken into consideration for applying AgNPs in enhancing radiation dose where nanoparticles containing greater X-ray attenuation were applied.
Deep Multi-Source Supervised Domain Adaptation with Class Imbalance
Authors: Ranvier Thomas , Elghazel Haytham , Coquery Emmanuel , Benabdeslem Khalid 
Published: July 2023

Abstract

Deep multi-source domain adaptation is based on deep neural networks and exploits knowledge from multiple source domains to improve predictions on a target domain.In this paper, we are especially interested in investigating domain adaptation in a supervised context, with limited data and class imbalance. We propose a new multi-source supervised domain adaptation approach that is able to transfer both shared knowledge across all source domains and source domain specific knowledge toward a target domain. Transfer contribution weights are computed during training based on domain divergence. They are used to balance each source domain impact on learning during the model training phase, limiting negative transfer as much as possible. We conduct extensive experiments to show that our approach competes and even outperforms other state-of-the-art domain adaptation approaches on both image benchmark datasets and real-world tabular medical data. We perform statistical analysis to better evaluate our experimental results, and conduct an ablation study to evaluate the usefulness of each component of the method.
Influence of Isomeric Composition and Sample Handling on the Liquid Density of Hydrofluorethers Measured by Vibrating Tube Densimeter at 0.1 MPa
Authors: Prokopová Olga , Blahut Aleš , Hajduch Jan , Kučnirová Kateřina , Čenský Miroslav , Aminian Ali , Richter Markus , Vinš Václav 
Published: July 2023

Abstract
Hydrofluoroethers (HFEs) represent a new family of promising engineering fluids suitable for technical cleaning, cooling of electronic and magnetic devices or as admixtures in refrigerant blends. Here we report accurate data for the liquid density at 0.1\,MPa and temperatures from $273.15\,\mathrm{K}$ to $343.15\,\mathrm{K}$ for a series of five HFEs; namely HFE-7000, HFE-7100, HFE-7200, HFE-7300, and HFE-7500. A highly sensitive vibrating tube densimeter with a borosilicate glass U-tube calibrated according to the procedure by Prokopov\'{a} et al. [J. Chem. Thermodyn. 173 (2022) 106855] provided density data with an expanded uncertainty ($k=2$) of $0.13\,\mathrm{kg\cdot m^{-3}}$. Influences such as sample degassing, water content or sample temperature before its dosing into the densimeter are discussed. Thanks to the high sensitivity of the used densimeter, an unexpected shift in the density of different HFE-7100 and HFE-7200 liquid samples was detected. Unlike other HFEs, HFE-7100 and HFE-7200 are mixtures of two hardly separable isomers, which were so far considered having identical thermophysical properties. Utilizing nuclear magnetic resonance spectroscopy, the ratio of \textit{n}-isomer and \textit{iso}-isomer was inspected for various liquid samples. In the range of \textit{iso}-isomer mole fraction from 0.61 to 0.77, the new measurements revealed density differences of more than $5\,\mathrm{kg\cdot m^{-3}}$ in case of HFE-7100 and of about $3\,\mathrm{kg\cdot m^{-3}}$ in case of HFE-7200. Consequently, for some applications the properties of different HFE isomers cannot be considered identical. The Rackett-type correlation for the saturated liquid density was fitted using the new and the literature data.