bluuebunny/crossref_metadata_2025_split huggingface dataset:
Future benefits of emission controls in mitigating climate-driven regional transport of PM2.5 in China
Authors: Li Siwei , Wang Shansi , Xing Jia , Ding Yu , Hu Senlin , Liu Shuchang , Qin Yu , Dong Zhaoxin , Dong Jiaxin , Song Ge , Dong Lechao , Fan Chunying , Teng Mengfan , Han Ge , Ma Xin , Lu Jinjun 
Published: May 2023

Abstract
Regional transport is equally significant to local sources in contributing to PM2.5 pollution and associated environmental inequality. In the context of future climate change, the effect of the responses of regional transport to the warming climate and controlling emissions has not been thoroughly investigated. Here we establish cross-province PM2.5 source-receptor matrix in China in 2015 baseline and two climate pathways in 2050s (SSP585 and SSP126), using Community Multi-scale Air Quality model embedded with the Integrated Source Apportionment Method. Results suggest that across-regional transport contributes 36.1 % - 62.3 % of PM2.5 in five severely polluted regions, which is even more important compared to inner transport within the target region (3.8 % - 19.4 %), especially in Chuanyu and Fenwei regions which are polluted by large PM2.5 transport (over 50 %) from outside regions. Such results imply that joint-control policy should not only focus on neighboring provinces. Controlling pollutant emission is undoubtedly reduced the concentration of PM2.5 regional transport (by -5.25 µg/m3 on average), largely exceeding the influence from the meteorological fluctuations (by -0.82 µg/m3 on average) driven by the climate change in 2050s. On the other hand, future controlled pollutant emissions proved that strengthen across-regional transport with an enlarged relative contribution to total PM2.5 concentration (8.46%) in 2050, along with a decreased contribution from local sources (8.54%). Therefore, environmental inequality issue should be pay more attention in the context of the reduction of pollution and carbon emissions.
GeNepher data- and biobank for patients with (suspected) genetic kidney disease: rationale, design and status update
Authors: Claus Laura R. , Zwaag Bert van der , Nguyen Tri Q. , Knoers Nine V.A.M. , de Borst Martin H. , Rookmaker Maarten B. , Lilien Marc R. , Eerde Albertien M. van , Contributors GeNepher Biobank 
Published: May 2023

Abstract

Background Clinical research on genetic kidney disease is thriving and the need for large cohorts, prospective data collection and biobanking is increasing. We aim to create a sustainable large genetic kidney disease biobank with a vast amount of uniformly collected high-quality data that is readily available for future research, with an infrastructure that allows for recontacting participants.Methods The GeNepher data- and biobank is an ongoing data- and sample collection that includes patients and family members with known and/or suspected genetic kidney disease. With a tiered approach participants can give broad consent for including their 1) available medical data (including genetic testing results), 2) inclusion of massively parallel sequencing data for add-on analysis, and 3) additional biobank sampling (e.g. urine for tubuloids, skin biopsy for fibroblasts). Recontacting is possible for additional data collection, novel research opportunities and return of relevant findings.Discussion The GeNepher data- and biobank collects prospective and retrospective data from kidney disease patients and their relatives. The broad consent allows for research that extends beyond one specific research question. Herewith, this biobank aims to 1) increase the scientific knowledge based on disease mechanisms including (novel) monogenic causes, 2) study modifiers, 3) improve care, including reproduction related research questions. Furthermore, it facilitates recontacting for opportunities in treatment development or when diagnose specific trials are started or specific treatment is approved.Conclusion The GeNepher biobank is designed to support a wide range of research projects by providing access to a diverse population of patients with (suspected) genetic kidney disease and has the potential to make a significant contribution to the field of rare kidney disease research.
Thrombin Worsens Extravascular Lung Water and Outcomes of Septic patients with Acute Respiratory Distress Syndrome
Authors: Chung Fu-Tsai , Kuo Chih-Hsi , Wang Chun-Hua , Lin Shu-Min 
Published: May 2023

Abstract
Introduction: Endothelial cell (EC) activation may increase systemic vascular permeability caused an extravasation of water (EVLW) in sepsis with acute respiratory distress syndrome (ARDS). The correlation of thrombin and EVLW in sepsis and ARDS hasn’t addressed.Methods Septic patients with ARDS were prospectively enrolled during 2014 and 2016, and EVLW and serum thrombin on day 1 and 3 were measured and compared between those with survival and non-survival. Additionally, morphologic change of human umbilical vein endothelial cells (HUVECs) with serum from patients with high and low EVLW was conducted.Results Twenty-seven patients were enrolled and the baseline characters including age, gender, APACHE II, prior 24hrs fluid balance, BMI, and shock status were similar between survivors and non-survivors. But day 1 EVLW was higher in non-survivor (27.5 ± 8.4 vs. 22 ± 6.5 ml/kg, p = 0.049). EVLW of survivor was improved from day 1 to day 3 (22 ± 6.5 vs. 11 ± 3.8 ml/kg, p = 0.0001), but it did not improve in non-survivor (27.5 ± 8.4 vs. 28 ± 6.7 ml/kg, p = 0.89). Thrombin level of survivor significantly improved (1.03 ± 0.55 vs. 0.87 ± 0.25 U/ml, p = 0.04) but it did not improve in non-survivor (1.97 ± 0.75 vs. 2.2 ± 0.75 U/ml, p = 0.07) from day 1 to day 3. EVLW and thrombin revealed a positive correlation (r2 = 0.71, p < 0.0001). In vitro, the morphology and junction of HUVECs were changed when serum of patients with high EVLW was added, but those did not change when serum of patients with low EVLW was added.Conclusion The level of EVLW and thrombin correlated positively and may impact the survival of these patients. Thrombin inhibitor may act as a novel valuable approach for preventing endothelial hyper-permeability and provides the rational basis for the use of this kind of drug in patients with severe sepsis and ARDS.
Reinforcing decellularized small intestine submucosa with cellulose acetate nanofibrous and silver nanoparticles as a scaffold for wound healing applications
Authors: Zavareh Zahra Khorasani , Asbagh Reza Akbari , Hajikhani Kiana , Tabasi Asieh Heirani , Nazari Hojjatollah , Abbasi Maryam , Moghaddam Mohammadamir Ghasemian , Behboudi Behanm , Kazemeini Alireza , Tafti Seyed Mohsen Ahmadi 
Published: May 2023

Abstract
Purpose The formation of chronic wounds accounts for considerable costs in health care systems. Despite the several benefits of decellularized small intestinal submucosa (SIS) as an appropriate scaffold for different tissue regeneration, it has shortcomings such as lack of antibacterial features and inappropriate mechanical properties for skin tissue regeneration. We aimed to examine the efficacy and safety of decellularized SIS scaffold enhanced with cellulose acetate (CA) and silver (Ag) nanoparticles (NPs) for healing full-thickness wounds.Methods The scaffolds were prepared by decellularizing bovine SIS and electrospinning CA/Ag nanoparticles and characterized using a transmission electron microscope (TEM), scanning electron microscope (SEM), tensile testing, and X-ray diffraction. In vivo evaluations were performed using full-thickness excisions covered with sterile gauze as the control group, SIS, SIS/CA, and SIS/CA/Ag scaffolds on the dorsum of twenty male Wistar rats divided into four groups randomly with 21-days follow-up. All in vivo specimens underwent Masson's trichrome (MT) staining for evaluation of collagen deposition, transforming growth factor-β (TGF-β) immunohistochemistry (IHC), and Haematoxylin Eosin(H&E) staining. The IHC and MT data were analyzed with the ImageJ tool by measuring the stained area.Results The TEM results revealed that Ag nanoparticles are successfully incorporated into CA nanofibers. Assessment of scaffolds hydrophilicity demonstrated that the contact angle of SIS/CA/Ag scaffold was the lowest. The in vivo results indicated that the SIS/CA/Ag scaffold had the most significant wound closure. H&E staining of the in vivo specimens showed the formation of epidermal layers in the SIS/CA/Ag group on day 21. The percentage of the stained area of MT and TGF-β IHC staining’s was highest in the SIS/CA/Ag group.Conclusion The decellularized SIS/CA/Ag scaffolds provided the most significant wound closure compared to other groups and caused the formation of epidermal layers and can be considered a novel method for enhancement of healing full-thickness wounds. Additionally, the collagen deposition and expression of TGF-β increased significantly in SIS/CA/Ag group.
Association Between Past Hepatitis B Infection and abdominal aorta calcification: National Health and Nutrition Examination Survey
Authors: Zhang kai , Cao Xiaoxiao , Gu Fangming , Gu Zhaoxuan , Han Yu , Hou Zhengyan , Yu Xiaoqi , Cai Tianyi , Gao Yafang , Xie Jinyu , Chen Jianguo , Chen Bowen , Liu Tianzhou 
Published: May 2023

Abstract
Introduction:
With mounting evidence, there is a postulation that Hepatitis B Infection may be a risk factor for atherosclerotic diseases. Abdominal aortic calcification (AAC) has been found to be significantly associated with subclinical atherosclerotic diseases. Our objective was to investigate the relationship between Hepatitis B core antibody and abdominal aortic calcification.
Methods:
In this cross-sectional observational study, data from the National Health and Nutrition Examination Survey (NHANES) conducted during 2013–2014 were utilized to investigate the relationship between Hepatitis B core antibody and AAC. The study used dual-energy X-ray absorptiometry to measure AAC and evaluated it using the Kauppila score system. The variable LBXHBC of the NHANES dataset was reviewed to identify patients with positive Hepatitis B core antibody. To assess the association between Hepatitis B core antibody and AAC, multivariate logistic regression mixed models and subgroup analyses were conducted.
Results:
The 2013–2014 data showed documentation of 288 patients with positive Hepatitis B core antibody. After adjusting for multiple variables, the multivariable odds ratios (95% CI) revealed that severe AAC was correlated with Hepatitis B infection (OR = 1.52, 95%CI [1 ~ 2.3]; p = 0.05), as opposed to the no Hepatitis B group. However, this association was not observed among individuals with mild AAC (OR = 1.22, 95%CI [0.9 ~ 1.65]; p = 0.205). Results were consistent in multiple subgroup and sensitivity analyses.
Conclusions:
Individuals who tested positive for Hepatitis B core antibody had a higher likelihood of severe AAC compared to those who tested negative, while no significant association was observed among individuals with mild AAC.
Universal adaptive optics for microscopy through embedded neural network control
Authors: Booth Martin , Hu Qi , Hailstone Martin , Wang Jingyu , Wincott Matthew , Stoychev Danail , Atilgan Huriye , Gala Dalia , Chaiamarit Tai , Parton Richard , Antonello Jacopo , Packer Adam , Davis Ilan 
Published: May 2023

Abstract
The resolution and contrast of microscope imaging is often affected by aberrations introduced by imperfect optical systems and inhomogeneous refractive structures in specimens. Adaptive optics (AO) compensates these aberrations and restores diffraction limited performance. A wide range of AO solutions have been introduced, often tailored to a specific microscope type or application. Until now, a universal AO solution – one that can be readily transferred between microscope modalities – has not been deployed. We propose versatile and fast aberration correction using a physics-based machine learning assisted wavefront-sensorless AO control (MLAO) method. Unlike previous ML methods, we used a bespoke neural network (NN) architecture, designed using physical understanding of image formation, that was embedded in the control loop of the microscope. The approach means that not only is the resulting NN orders of magnitude simpler than previous NN methods, but the concept is translatable across microscope modalities. We demonstrated the method on a two-photon, a three-photon and a widefield three-dimensional (3D) structured illumination microscope. Results showed that the method outperformed commonly-used model-based sensorless AO methods. We also showed that our ML-based method was robust in a range of challenging imaging conditions, such as extended 3D sample structures, specimen motion, low signal to noise ratio and activity-induced fluorescence fluctuations. Moreover, as the bespoke architecture encapsulated physical understanding of the imaging process, the internal NN configuration was no-longer a “black box”, but provided physical insights on internal workings, which could influence future designs.
Extensive studies on the Electrochemical, Photocatalytic and Antimicrobial Applications of Bio assisted Nano Titania
Authors: N Saikumari , S Sudhakhar K 
Published: May 2023

AbstractIn recent days synthesis and structuring of intelligent nano materials investigated and reported has developed critical scientific ideas to the greater extent. The excellent thermal, optical and electrical properties along with its resistant to corrosion, wear, oxidation and erosion made it unique for sustainable environmental applications. Here nano structured Titanium di oxide particles synthesized from its precursor via template assisted sol-gel technique have been verified as corrosion inhibitor of brass alloy in acid medium along with its photo catalytic and anti-microbial applications. The physico-chemical parameters of the synthesized nano materials were studied using XRD, FT-IR, UV-DRS, SEM, TEM and BET analytical techniques and revealed the impact of tea leaf extract as a template in producing a nano catalyst NTG about 14 nm in size with tailored structural, optical and morphological characteristics. The rate of corrosion of a brass strip in acid medium is verified by weight loss method and the inhibitor efficiency increased with the increase in concentration of the nano catalyst. The catalytic activity is proved against the photo degradation of a toxic melamine, a trimer of cyanamide. The synthesized nano catalyst showed excellent antimicrobial properties proven against the growth of K. pneumonia and H. influenza.
Use of Medication for Preterm Labor: Retrospective Cohort Study in a Single Tertiary Center in Wuhan, China
Authors: Lv Dan , Zhang Yanling , Zhang Xiaolei , Li Xufang , Jallow Fatoumata , Li Fei , Xie Yin , Li Fanfan , He Mengzhou , Fan Yao , Li Wei , Feng Ling , Lin Xingguang , Deng Dongrui 
Published: May 2023

Abstract
Background Prevention and treatment in preterm birth are still under intensive investigation. A gap exists between evidence-based recommendations and clinical practice. A deeper understanding of the prevalence of medication use is an essential step toward improving the care of pregnant women. This study aimed to address this issue.Methods A retrospective cohort was conducted between December 2018 and November 2019 in Tongji Hospital (Wuhan). In total, 878 pregnant women were enrolled. Information on maternal characteristics, medication prescription, maternal outcomes (including post-partum hemorrhage), and neonatal outcomes (including APGAR score, birthweight, neonatal intensive care unit (NICU) admission, cardiopulmonary and neurological diseases) in the linking system were retrieved.Results The overall coverage of the common medications, including antenatal corticosteroids (ACS), magnesium sulfate, tocolytics, and progesterone, was 84.5%, 52.8%, 24.9%, and 13.3%, respectively. The treatment plan varied widely regarding the regimen, dosing, duration, and combination of these drugs. Specifically, tocolytic maintenance, which means the administration duration of more than 48 hours in this study, increased the risk of post-partum hemorrhage; it also significantly increased birthweight, but was not a risk factor of large-for-gestational-age (LGA).Conclusions This retrospective study in a single tertiary center disclosed that obstetric medications for preterm labor are highly individualized and do not strictly conform to current guidelines. This phenomenon is not uncommon in other countries. Clinical practice and evidence-based recommendations need to be carefully balanced; more studies are warranted to provide high-quality evidence to aid clinical decisions.
Behavior diversity in Odonata larvae is increased by water turbidity
Authors: Palacino-Rodríguez Fredy , Silva-Brito Joas , Juen Leandro , Palacino-Penagos Diego Andrés 
Published: May 2023

Abstract
Aquatic insects’ behavior changes due to physiological constraints, trophic interactions, habitat selection, and biotic interactions. Addressing these topics can help to potentiate our understanding of ecosystem services and community structure. Here, the larval behavior of seven Odonata species was studied in the laboratory to evaluate variations in frequency associated with sex and three types of water: drinking water, dechlorinated tap water, and water from the collection habitat. Larvae were maintained into containers at ambient temperature, 12h light, and food ad libitum. Larval behavior included motionless, swimming, feeding, body movements, walking, grooming, changes in the body orientation, perching, molting, suspending, and sitting and waiting to capture prey. Larvae showed the highest number of behaviors in higher turbidity water (Habitat Water treatment). Males and females showed similar frequency in behaviors. The more active were of the Sympetrum gilvumand Rhionaeschna cornigera species. Species showed higher frequencies in behaviors such as resting, eating, prey capture (Anisoptera), perching and walking (Zygoptera). The frequency of larval behaviors in higher turbidity is modulated by the capability to obtain food and simultaneously, avoid predators. Larvae show higher diversity of behaviors in increased turbidity because darker habitats could be more secure. Our study calls attention to the importance of addressing the effect of abiotic conditions on behaviors of aquatic insects, and how it can influence their ecological fitness. Finally, although we have achieved crucial advancements on molecular tools and sophisticated statistical routines, the basic information about behaviors facets it is still a need in ecological studies.
Alfuzosin vs. Silodosin in the Improvement of Ureteral Stent-Related Symptoms after Endoscopic Surgical Treatment of Urolithiasis: A Retrospective Comparison
Authors: Sirin Mehmet Emin , Yilmaz Mehmet , Polat Muhammed Emin , Olcucuoglu Erkan , Karaaslan Mustafa 
Published: May 2023

Abstract
Purpose In this study, we aimed to compare the efficacy of two α-blockers, alfuzosin and silodosin, which have different pharmacological properties despite being uroselective, in the treatment of double-J stent (DJS)-related symptoms.Material and methods Fifty male patients who underwent DJS implantation after ureterorenoscopy/retrograde intrarenal surgery were included in the study. The patients’ demographic and characteristic data were analyzed. All patients were asked to complete the validated Turkish version of the Ureteral Stent Symptom Questionnaire (USSQ), international prostate symptom score (IPSS), and international index of erectile function-5 (IIEF-5) the day before stent removal and again one month after stent removal. Statistical analysis of these variables was performed in the alfuzosin and silodosin groups, and a value of p < 0.05 was considered statistically significant.Results Whereas no side effects were observed in the alfuzosin group (Group A), retrograde ejaculation was observed in 8 (25.2%) patients in the silodosin group (Group S) (p = 0.006). Significant decreases in IPSS, IPSS-quality of life, and USSQ scores were observed in both Group A and Group S one month after stent removal (i.e., without stent) compared with before stent removal (i.e., with stent) (p < 0.05). Furthermore, a statistically significant increase in IIEF-5 score was observed in both groups after stent removal compared with IIEF-5 score with stenting (p < 0.05).Conclusion The DJS has a significant impact on patients’ quality of life and should only be used when indicated, not routinely after every endoscopic stone procedure. Considering that silodosin may cause more ejaculation problems than alfuzosin, we believe that both drugs can be used in male patients to resolve DJS-related symptoms.
The DNA damage response to radiological imaging: from ROS and γH2AX foci induction to gene expression responses in vivo
Authors: López-Riego Milagrosa , Płódowska Magdalena , Lis-Zajęcka Milena , Jeziorska Kamila , Tetela Sylwia , Węgierek-Ciuk Aneta , Sobota Daniel , Braziewicz Janusz , Lundholm Lovisa , Lisowska Halina , Wojcik Andrzej 
Published: May 2023

Abstract
Candidate ionising radiation exposure biomarkers must be validated in humans exposed in vivo. Blood from patients undergoing positron emission tomography - computed tomography scan (PET-CT) and skeletal scintigraphy (scintigraphy) was drawn before (0 h) and after (2 h) the procedure for correlation analyses of response of selected biomarkers with radiation dose and other available patient information. FDXR, CDKN1A, BBC3, GADD45A, XPCand MDM2 expression was determined by qRT-PCR, DNA damage (γH2AX) by flow cytometry, and reactive oxygen species (ROS) levels by flow cytometry using the 2', 7' – Dichlorofluorescin diacetate test in peripheral blood mononuclear cells (PBMC). For ROS experiments, 0- and 2 h samples were additionally exposed to UVA to determine whether diagnostic irradiation conditioned the response to further oxidative insult.
With some exceptions, radiological imaging induced weak γH2AX foci, ROS and gene expression fold changes, the latter with good coherence across genes within a patient. Diagnostic imaging did not influence oxidative stress in PBMC successively exposed to UVA. Correlation analyses with patient characteristics led to low correlation coefficient values. γH2AX fold change, which correlated positively with gene expression, presented a weak positive correlation with injected activity, indicating a radiation-induced subtle increase in DNA damage and subsequent activation of the DNA damage response pathway. The exposure discrimination potential of these biomarkers in the absence of control samples, as frequently demanded in radiological emergencies, was assessed using raw data. These results suggest that the variability of the response in heterogeneous populations might complicate identifying individuals exposed to low radiation doses.
Regulation of CagA-Helicobacter on Gastric PIM2 Expression in Gastric Cancer
Authors: Wang Haiyan , Cao Wenting , Lijuan Hong , Zhang Huijuan , Ni Peng , Chen Shuaiyin , Duan Guangcai , Liang Wenjuan , Zhang Rongguang 
Published: May 2023

AbstractInfection with cagA-positiveH. pylori has been linked to an increased risk of gastric cancer. However, the precise mechanism by whichcagAregulates PIM2 expression in gastric cancer is currently unknown. To address this issue, a mutant NCTC11637ΔcagA strain ofH. pyloriwas constructed, and the effects of H. pylori/cagA on PIM2 expression in gastric cancer cells (HGC27, SGC7901, and AG) were examined. The results showed that co-culturing gastric cancer cells with NCTC11637 significantly increased PIM2 expression levels (P < 0.001) compared to the control group. Additionally, the expression of PIM2 in cells co-cultured with NCTC11637 was higher than that co-cultured with NCTC11637ΔcagA (P < 0.001). Furthermore, the eukaryotic expression vector pcDNA-cagA was successfully constructed, and its transfection into gastric cancer cells resulted in a significant increase in PIM2 mRNA expression levels compared to the control group after 48 hours. These findings suggest that H.pylori/CagA may play a crucial role in regulating PIM2 expression and therefore may contribute to the pathogenesis ofH. pyloriinfection.