bluuebunny/crossref_metadata_2025_split huggingface dataset:
Temperature-dependent spawning behaviour and larval thermohaline associations of Bering Sea groundfish
Authors: Vary L , Rogers L , Harte M , Howard R , Ciannelli L 
Published: July 2023

Abstract
The Bering Sea is a productive large marine ecosystem that supports numerous commercial fisheries, while climate change is introducing rapid warming and freshening, especially in coastal water. The success of early life stages of marine fish can impact adult abundance levels; little is known about how behavioural or physiological plasticity in relation to environmental changes at one stage (e.g. spawning) may affect survival during subsequent stages (e.g. larvae), nor whether trade-offs exist that affect how a species demonstrates such plasticity. We utilized a statistical approach to examine phenological and geographical flexibility in spawning behaviour for four species of groundfish. In situ sea surface temperature (SST) and salinity (SSS) associations were also estimated by statistical models for six species of groundfish larvae. All species exhibited greater spawning geography flexibility than phenological flexibility during the egg stage. All larval stages exhibited specific temperature and salinity associations across unique combinations of SST and SSS. These species-specific patterns, in the context of potential climate change impacts, suggest that flexibility in spawning behaviour may not adequately compensate for the presence of unfavourable habitats at the larval stage.
Parallel And Divergent Morphological Adaptations Underlying The Evolution of Jumping Ability in Ants
Authors: Aibekova L , Keller R A , Katzke J , Allman D M , Hita-Garcia F , Labonte D , Narendra A , Economo E P 
Published: July 2023

SynopsisJumping is a rapid locomotory mode widespread in terrestrial organisms. However, it is a rare specialization in ants. Forward jumping has been reported within four distantly related ant genera: Gigantiops, Harpegnathos, Myrmecia, and Odontomachus. The temporal engagement of legs/body parts during jump, however, varies across these genera. It is unknown what morphological adaptations underlie such behaviors and whether jumping in ants is solely driven directly by muscle contraction or additionally relies on elastic recoil mechanism. We investigated the morphological adaptations for jumping behavior by comparing differences in the locomotory musculature between jumping and non-jumping relatives using X-ray micro-CT and 3D morphometrics. We found that the size-specific volumes of the trochanter depressor muscle (scm6) of the middle and hind legs are 3–5 times larger in jumping ants, and that one coxal remotor muscle (scm2) is reduced in volume in the middle and/or hind legs. Notably, the enlargement in the volume of other muscle groups is directly linked to the legs or body parts engaged during the jump. Furthermore, a direct comparison of the muscle architecture revealed two significant differences between jumping vs. non-jumping ants: First, the relative Physiological Cross-Sectional Area (PCSA) of the trochanter depressor muscles of all three legs were larger in jumping ants, except in the front legs of Odontomachus rixosus and Myrmecia nigrocincta; second, the relative muscle fiber length was shorter in jumping ants compared to non-jumping counterparts, except in the front legs of O. rixosus and M. nigrocincta. These results suggest that the difference in relative muscle volume in jumping ants is largely invested in the area (PCSA), and not in fiber length. There was no clear difference in the pennation angle between jumping and non-jumping ants. Additionally, we report that the hind leg length relative to body length was longer in jumping ants. Based on direct comparison of the observed vs. possible work and power output during jumps, we surmise that direct muscle contractions suffice to explain jumping performance in three species, except for O. rixosus, where the lack of data on jumping performance prevents us from drawing definitive conclusions for this particular species. We suggest that increased investment in jumping-relevant musculature is a primary morphological adaptation that separates jumping from non-jumping ants. These results elucidate the common and idiosyncratic morphological changes underlying this rare adaptation in ants.まとぅみ (Okinawan language—Uchinaaguchi)要旨 (Japanese)РЕЗЮМЕ (Kazakh)ZUSAMMENFASSUNG (German)
Diagnostic and antibiotic stewardship lessons: an outpatient assessment of symptomatic reflex urinalysis ordering accuracy using an electronic best-practice alert
Authors: Nguyen Hien M , Flerchinger Shaun , Smith Jeffrey R , Felcher Andrew H , Turley Marianne , Mcnamara Michael 
Published: July 2023

Abstract

Background
It is not well known how reliably clinicians order reflex urinalysis to microscopy and culture (rUA-cx) for outpatient urinary tract infection (UTI) workup. Antibiotic appropriateness cannot be fully appreciated until the prevalence of UTIs and asymptomatic bacteriuria (ASB) are realized.

Objective
This quality improvement study has two major aims, first to determine UTI symptom accuracy for rUA-cx ordering and second, to confirm UTI and ASB cases by integrating rUA-cx and cascaded urinalysis results. Antibiotic utilization and diagnostic coding were secondarily linked to UTIs and ASB.

Methods
An electronic best-practice alert informed the ordering of two rUA-cx options: symptomatic- rUA-cx specifically for dysuria, frequency, urgency, costovertebral pain, suprapubic pain or fever versus non-specific-rUA-cx for vague complaints. UTI symptoms were verified by chart review. Confirmed UTI was defined as a significant culture with UTI symptoms and ASB as a significant culture without UTI symptoms.

Results
rUA-cx (2065) were prospectively collected over 6 months from female patients at risk for uncomplicated UTIs. Symptomatic-rUA-cx and non-specific-rUA-cx were associated with UTI symptoms for 53% (809/1527) and 20% (107/538), respectively. Overall, 44% (916/2065) of all rUA-cx had UTI symptoms. rUA-cx were overordered by a factor of 9 (2065/225) for every confirmed UTI. The UTI-to-ASB relative ratio was 2.6 (225/86). Regarding UTI-relevant antibiotics, 39% (214/553) were appropriately associated with UTI whereas only 22% (74/339) of inappropriate antibiotics were captured by the ASB definition, underestimating the problem 4-fold.

Conclusions
UTI and ASB remain challenging to categorize despite a meticulous method that applied acceptable criteria.
Structural insights into endothelin receptor signalling
Authors: Shihoya Wataru , Sano Fumiya K , Nureki Osamu 
Published: July 2023

Abstract
Endothelins and their receptors, type A (ETA) and type B (ETB), modulate vital cellular processes, including growth, survival, invasion and angiogenesis, through multiple G proteins. This review highlights the structural determinations of these receptors by X-ray crystallography and cryo-electron microscopy, and their activation mechanisms by endothelins. Explorations of the conformational changes upon receptor activation have provided insights into the unique G-protein coupling feature of the endothelin receptors. The review further delves into the binding modes of the clinical antagonist and the inverse agonists. These findings significantly contribute to understanding the mechanism of G-protein activation and have potential implications for drug development, particularly in the context of vasodilatory antagonists and agonists targeting the endothelin receptors.
Successful establishment, spread, and impact of the introduced parasitoid Spathius galinae (Hymenoptera: Braconidae) on emerald ash borer (Coleoptera: Buprestidae) populations in postinvasion forests in Michigan
Authors: Duan Jian J , Schmude Jonathan M , Petrice Toby R , Bauer Leah S , Poland Therese M , Chandler Jennifer L , Crandall Ryan , Elkinton Joseph S , Van Driesche Roy 
Published: July 2023

Abstract
Spathius galinae is a larval parasitoid native to the Russian Far East that was approved for release in the United States in 2015 for biological control of the emerald ash borer (EAB), Agrilus planipennis, an invasive beetle from Asia responsible for widespread mortality of ash trees (Fraxinus spp.) in North America. From 2015 to 2017, 1,340–1,445 females of S. galinae along with males were released into each release plot, paired with a nonrelease control plot (1–12.5 km apart), at 6 postinvasion forested sites containing abundant pole-sized ash trees in Michigan. By 2018, S. galinae had spread to all but one control plot. Based on the first year that S. galinae was found in trees in each control plot and the distances of those trees to the parasitoid release point within each site, we estimated that S. galinae spread at 3.7 (±1.9) km per year after its initial releases in 2015. The proportion of sampled trees with S. galinae broods, brood densities within sampled trees, and parasitism of EAB larvae increased sharply in both control and release plots after the last field releases in 2017, with the highest parasitism rates (42.8–60.3%) in 2020. Life table analysis showed that S. galinae alone reduced EAB’s net population growth rate by 35–55% across sites from 2018 to 2020. These results demonstrate that S. galinae has established an increasing population in Michigan and now plays a significant role in reducing EAB populations in the area.
Forced Migration and Local Public Policies: Evidence from Post-War West Germany
Authors: Chevalier Arnaud , Elsner Benjamin , Lichter Andreas , Pestel Nico 
Published: July 2023

Abstract
We study the effect of forced migration on public policy setting in the migrant-receiving country. After World War II, eight million expelled Germans arrived in West Germany within 5 years. We use regional variation in the population share of forced migrants across West German cities to estimate the effect of this inflow on cities’ taxation and spending decisions. To identify a causal effect, we pursue an instrumental variable strategy that leverages push factors of the expulsions while being orthogonal to local conditions in the destination regions. Our results show that cities with high inflows of forced migrants increased spending on welfare and education, decreased spending on infrastructure, raised local taxes, and incurred more debt. Part of these effects can be attributed to shifts in political preferences. The migrants held voting rights upon arrival and supported parties that explicitly catered to their interests and needs.
Towards a Shared Practice of Encampment: An Historical Investigation of UNRWA and the UNHCR to 1967
Authors: Robson Laura 
Published: July 2023

AbstractThis article reconsiders refugee studies’ longstanding commitment to the notion of a near-total separation between the UNHCR and UNRWA through an historical investigation of both organizations’ early approaches to refugee encampment. Without disputing the importance of the historical and institutional divisions between the two, it seeks to point out that with respect to the practice of encampment, the two agencies gradually drew nearer to each other during their first two decades of operation, as the UNHCR extended its reach into the decolonizing world and UNRWA expanded its operations across the Middle East. The parallels became particularly clear in 1967, when the ‘Protocol on the Status of Refugees’ formalized the UNHCR’s encampment-focused expansion into the decolonizing world at roughly the same time that the second Arab-Israeli war created the conditions for UNRWA to enshrine encampment as a more or less permanent institutional strategy.
Development and validation of a new quantitative reverse transcription PCR assay for the diagnosis of human sporotrichosis
Authors: Marques de Macedo Priscila , Sturny-Leclère Aude , Freitas Dayvison Francis Saraiva , Ghelfenstein-Ferreira Theo , Gutierrez-Galhardo Maria Clara , Almeida Marcos de Abreu , Rodrigues Anderson Messias , Pautet Thierry , Hamane Samia , Almeida-Paes Rodrigo , Zancopé-Oliveira Rosely Maria , Alanio Alexandre 
Published: July 2023

Abstract
Sporotrichosis is an emergent public health problem. The mycological diagnosis of this infection is based on culture, which is fastidious and may represent a biohazard for technicians. Although not widely implemented in routine diagnosis, molecular methodologies are fast, have good accuracy, and can be easily standardized, aiding in the early diagnosis of neglected mycoses. This study aimed at implementing a new pan-Sporothrix quantitative reverse transcription PCR (RT-qPCR) assay, and then validating it on clinical samples from confirmed human sporotrichosis cases. A total of 68 human samples with culture-confirmed diagnosis of sporotrichosis were collected from 64 patients followed at a Brazilian reference center for endemic mycoses. These samples were submitted to whole nucleic acid extraction, followed by an RT-qPCR protocol. The limit of detection was 244 fg, the efficiency was 2.0 (100%), and the assay could amplify the genetic material of the three major clinically relevant species of the genus Sporothrix. Among the 68 samples analyzed, 62 were positive in RT-qPCR, showing an overall sensitivity of 91.18%, which variated according to the type of biological sample: 96.72% in skin samples (n = 61) and 100% in respiratory samples (n = 3), whereas all cerebrospinal fluid specimens (n = 4) were negative. The specificity was 100% when tested in 25 samples from patients with other mycoses and tuberculosis. In addition, DNA from 93 fungal species did not yield positive results, confirming the high specificity of this test. Our RT-qPCR presented high sensitivity and specificity, representing an excellent tool for a fast and reliable diagnosis of human sporotrichosis.
Aspergillus sensitisation detection using point-of-care lateral flow assay in moderate to severe asthma
Authors: Wang Ran , Eades Chris , Palmer Maisie , Platt Gareth , Fowler Stephen J , Kosmidis Chris 
Published: July 2023

Abstract
Allergic fungal airway diseases are associated with asthma exacerbations and poor control. However, the early identification of allergic Aspergillus airway diseases can be challenging, especially in resource-poor countries. We aimed to evaluate the clinical utility of the point-of-care Aspergillus IgG–IgM lateral flow assay in diagnosing Aspergillus airway diseases in patients with moderate–severe asthma. Patients with moderate–severe asthma, severe asthma with fungal sensitisation (SAFS) and allergic bronchopulmonary aspergillosis (ABPA) were recruited. Clinical information was extracted from clinical records. Blood samples were collected for serological tests. Serum samples were evaluated using Aspergillus immunochromatographic test (ICT). A total of 65 patients were recruited into the study, of whom 23.1% had clinical diagnosis of ABPA, 18.5% had SAFS and 58.5% had moderate-to-severe asthma who did not fit ABPA or SAFS criteria. The ICT test gave a sensitivity of 69 [95% confidence interval: 51–88]% and a specificity of 77 [60–88]% in predicting a positive Aspergillus IgG test. The sensitivity and specificity for a positive Aspergillus IgE were 77 [59–88]% and 86 [71–94]%, respectively. The majority (sensitivity: 87 [62–96]%) of patients with ABPA had positive ICT results, with a specificity of 70%. The negative predictive value was high (95 [82–99]%) with a low negative likelihood ratio (&lt; 0.2), making it potentially useful in ruling out ABPA. The ICT assay may be valuable in ruling out ABPA in resource-limited countries where serological investigations are less feasible. The ICT assay may be particularly useful in ruling out ABPA and warrants further validation.
Decoding the compositions of four bright r-process-enhanced stars
Authors: Saraf Pallavi , Prieto Carlos Allende , Sivarani Thirupathi , Bandyopadhyay Avrajit , Beers Timothy C , Susmitha A 
Published: July 2023

ABSTRACT
There has been a concerted effort in recent years to identify the astrophysical sites of the r-process that can operate early in the galaxy. The discovery of many r-process-enhanced (RPE) stars (especially by the R-process Alliance collaboration) has significantly accelerated this effort. However, only limited data exist on the detailed elemental abundances covering the primary neutron-capture peaks. Subtle differences in the structure of the r-process pattern, such as the relative abundances of elements in the third peak, in particular, are expected to constrain the r-process sites further. Here, we present a detailed elemental-abundance analysis of four bright RPE stars selected from the HESP–GOMPA survey. Observations were carried out with the 10-m class telescope Gran Telescopio Canarias (GTC), Spain. The high spectral signal-to-noise ratios obtained allow us to derive abundances for 20 neutron-capture elements, including the third r-process peak element osmium (Os). We detect thorium (Th) in two stars, which we use to estimate their ages. We discuss the metallicity evolution of Mg, Sr, Ba, Eu, Os, and Th in r-II and r-I stars, based on a compilation of RPE stars from the literature. The strontium (Sr) abundance trend with respect to europium (Eu) suggests the need for an additional production site for Sr (similar to several earlier studies); this requirement could be milder for yttrium (Y) and zirconium (Zr). We also show that there could be some time delay between r-II and r-I star formation, based on the Mg/Th abundance ratios.
Evidence for mass-dependent peculiar velocities in compact object binaries: towards better constraints on natal kicks
Authors: Zhao Yue , Gandhi Poshak , Dashwood Brown Cordelia , Knigge Christian , Charles Phil A , Maccarone Thomas J , Nuchvanichakul Pornisara 
Published: July 2023

ABSTRACT
We compile a catalogue of low-mass and high-mass X-ray binaries, some recently reported binaries that likely host a neutron star (NS) or a black hole (BH), and binary pulsars (a pulsar and a non-degenerated companion) that have measured systemic radial velocities (γ). Using Gaia and radio proper motions together with γ, we integrate their Galactic orbits and infer their post-supernova (post-SN) 3D peculiar velocities ($v_\mathrm{pec }^{z=0}$ at Galactic plane crossing); these velocities bear imprints of the natal kicks that compact objects received at birth. With the sample totalling 85 objects, we model the overall distribution of $v_\mathrm{pec }^{z=0}$ and find a two-component Maxwellian distribution with a low- ($\sigma _v \approx 21\, \mathrm{km s^{-1}}$) and a high-velocity ($\sigma _v \approx 107\, \mathrm{km s^{-1}}$) component. A further comparison between distributions of binary subgroups suggests that binaries hosting high-mass donors/luminous companions mostly have $v_\mathrm{pec }^{z=0}\lesssim 100\, \mathrm{km s^{-1}}$, while binaries with low-mass companions exhibit a broader distribution that extends up to $\sim 400\, \mathrm{km s^{-1}}$. We also find significant anticorrelations of $v_\mathrm{pec }^{z=0}$ with binary total mass (Mtot) and orbital period (Porb), at over 99 per cent confidence. Specifically, our fit suggests $v_\mathrm{pec }^{z=0}\propto M_\mathrm{tot}^{-0.5}$ and $v_\mathrm{pec }^{z=0}\propto P_\mathrm{orb}^{-0.2}$. Discussions are presented on possible interpretation of the correlations in the context of kinematics and possible biases. The sample should enable a range of follow-up studies on compact object binary kinematics and evolution.
Rotational and radio emission properties of PSR J0738−4042 over half a century
Authors: Lower M E , Johnston S , Karastergiou A , Brook P R , Bailes M , Buchner S , Deller A T , Dunn L , Flynn C , Kerr M , Manchester R N , Mandlik A , Oswald L S , Parthasarathy A , Shannon R M , Sobey C , Weltevrede P 
Published: July 2023

ABSTRACT
We present a comprehensive study of the rotational and emission properties of PSR J0738−4042 using a combination of observations taken by the Deep Space Network, Hartebeesthoek, Parkes (Murriyang) and Molonglo observatories between 1972 and 2023. Our timing of the pulsar is motivated by previously reported profile/spin-down events that occurred in 2005 September and 2015 December, which result in an anomalously large braking index of n = 23 300 ± 1800. Using a Gaussian process regression framework, we develop continuous models for the evolution of the pulsar spin-down rate ($\dot{\nu }$) and profile shape. We find that the pulse profile variations are similar regardless of radio observing frequency and polarization. Small-scale differences can be ascribed to changes in the interstellar medium along the line of sight and frequency-dependent changes in magnetospheric radio emission height. No new correlated spin-down or profile events were identified in our extended data set. However, we found that the disappearance of a bright emission component in the leading edge of archival profiles between 1981 and 1988 was not associated with a substantial change in $\dot{\nu }$. This marks a notable departure from the previous profile/spin-down events in this pulsar. We discuss the challenges these observations pose for physical models and conclude that interactions between the pulsar and in-falling asteroids or a form of magnetospheric state-switching with a long periodicity are plausible explanations.
On the likelihoods of finding very metal-poor (and old) stars in the Milky Way’s disc, bulge, and halo
Authors: Sotillo-Ramos Diego , Bergemann Maria , Friske Jennifer K S , Pillepich Annalisa 
Published: July 2023

ABSTRACT
Recent observational studies have uncovered a small number of very metal-poor (VMP) stars with cold kinematics in the Galactic disc and bulge. However, their origins remain enigmatic. We select a total of 138 Milky Way (MW) analogues from the TNG50 cosmological simulation based on their z = 0 properties: discy morphology, stellar mass, and local environment. In order to make more predictive statements for the MW, we further limit the spatial volume coverage of stellar populations in galaxies to that targeted by the upcoming 4MOST high-resolution survey of the Galactic disc and bulge. We find that across all galaxies, ∼20 per cent of VMP ([Fe/H] &lt; −2) stars belong to the disc, with some analogues reaching 30 per cent. About 50 ± 10 per cent of the VMP disc stars are, on average, older than 12.5 Gyr and ∼70 ± 10 per cent come from accreted satellites. A large fraction of the VMP stars belong to the halo (∼70) and have a median age of 12 Gyr. Our results with the TNG50 cosmological simulation confirm earlier findings with simulations of fewer individual galaxies, and suggest that the stellar disc of the MW is very likely to host significant amounts of very- and extremely-metal-poor stars that, although mostly of ex situ origin, can also form in situ, reinforcing the idea of the existence of a primordial Galactic disc.
Nucleosome retention by histone chaperones and remodelers occludes pervasive DNA–protein binding
Authors: Jonas Felix , Vidavski Matan , Benuck Eli , Barkai Naama , Yaakov Gilad 
Published: July 2023

Abstract
DNA packaging within chromatin depends on histone chaperones and remodelers that form and position nucleosomes. Cells express multiple such chromatin regulators with overlapping in-vitro activities. Defining specific in-vivo activities requires monitoring histone dynamics during regulator depletion, which has been technically challenging. We have recently generated histone-exchange sensors in Saccharomyces cerevisiae, which we now use to define the contributions of 15 regulators to histone dynamics genome-wide. While replication-independent exchange in unperturbed cells maps to promoters, regulator depletions primarily affected gene bodies. Depletion of Spt6, Spt16 or Chd1 sharply increased nucleosome replacement sequentially at the beginning, middle or end of highly expressed gene bodies. They further triggered re-localization of chaperones to affected gene body regions, which compensated for nucleosome loss during transcription complex passage, but concurred with extensive TF binding in gene bodies. We provide a unified quantitative screen highlighting regulator roles in retaining nucleosome binding during transcription and preserving genomic packaging.