bluuebunny/crossref_metadata_2025_split huggingface dataset:
Optimising a self-assembling peptide hydrogel as a Matrigel alternative for 3-dimensional mammary epithelial cell culture
Authors: Lingard Eliana , Dong Siyuan , Hoyle Anna , Appleton Ellen , Hales Alis , Skaria Eldhose , Lawless Craig , Saadati Simon , Miller Aline F. , Domingos Marco , Saiani Alberto , Swift Joe , Gilmore Andrew P. 
Published: September 2023

ABSTRACTThree-dimensional (3D) organoid models have been instrumental in understanding molecular mechanisms responsible for many cellular processes and diseases. However, established organic biomaterial scaffolds used for 3D hydrogel cultures, such as Matrigel, are biochemically complex and display significant batch variability, limiting reproducibility in experiments. Recently, there has been significant progress in the development of synthetic hydrogels forin vitrocell culture that are reproducible, mechanically tuneable, and biocompatible. Self-assembling peptide hydrogels (SAPHs) are synthetic biomaterials that can be engineered to be compatible with 3D cell culture. Here we investigate the ability of PeptiGel® SAPHs to model the mammary epithelial cell (MEC) microenvironmentin vitro. The positively charged PeptiGel®Alpha4 supported MEC viability, but did not promote formation of polarised acini. Modifying the stiffness of PeptiGel® Alpha4 stimulated changes in MEC viability and changes in protein expression associated with altered MEC function, but did not fully recapitulate the morphologies of MECs grown in Matrigel. To supply the appropriate biochemical signals for MEC organoids, we supplemented PeptiGels® with laminin. Laminin was found to require negatively charged PeptiGel® Alpha7 for functionality, but was then able to provide appropriate signals for correct MEC polarisation and expression of characteristic proteins. Thus, optimisation of SAPH composition and mechanics allows tuning to support tissue-specific organoids.
ARF6 as a novel activator of HIF-2α in pulmonary arterial hypertension
Authors: Fellows Adam L. , Chen Chien-Nien , Xie Chongyang , Iyer Nayana , Schmidt Lukas , Yin Xiaoke , Mayr Manuel , Cowburn Andrew , Zhao Lan , Wojciak-Stothard Beata 
Published: September 2023

ABSTRACTADP-ribosylation factor 6 (ARF6), a GTPase associated with cancer metastasis, is activated in the lung endothelium in pulmonary arterial hypertension (PAH). To identify ARF6-regulated pathways relevant to PAH, we performed a state-of-the-art proteomic analysis of human pulmonary artery endothelial cells (HPAECs) overexpressing the wildtype, constitutively active, fast-cycling and dominant negative mutants of ARF6. The analysis revealed a novel link of ARF6 with hypoxia-inducible factor (HIF), in addition to endocytotic vesicle trafficking, cell proliferation, angiogenesis, oxidative stress and lipid metabolism. Active ARF6 markedly increased expression and activity of HIF-2, critical in PAH, with HIF-1 relatively unaffected. Hypoxic ARF6 activation was a prerequisite for HIF-2 activation and HIF-dependent gene expression in HPAECs, PAH blood-derived late outgrowth endothelial colony forming cells (ECFCs) and hypoxic mouse lungsin vivo. A novel ARF6 inhibitor, chlortetracycline (CTC), reduced hypoxia-induced HIF-2 activation, proliferation and angiogenesis in HPAECs and reduced HIF-2 expression in lung and heart tissues of hypoxic mice. PAH ECFCs showed elevated expression and activity of ARF6 and HIF2, which was attenuated by CTC. Oral CTC attenuated development of PH in chronically hypoxic mice. In conclusion, we are first to demonstrate a key role of ARF6 in the regulation of HIF-2α activationin vitroandin vivoand show that HIF-2α, a master-regulator of vascular remodelling in PAH, can be targeted by a clinically approved antibiotic chlortetracycline.
Instilling employees with meaning to communicate: servant leadership affecting employee voice behavior through meaning
Authors: Khan Muhammad Mumtaz , Mubarik Muhammad Shujaat , Ahmed Syed Saad , Ali Syed Rizwan , Siraj Syed Sajid 
Published: September 2023

Purpose
This study aims to analyze the connection between servant leadership and the promotive and prohibitive voice behavior of employees. In addition, this study explained how servant leadership affects promotive and prohibitive voice behavior through meaning.

Design/methodology/approach
For this study, data were collected from employee–manager dyads employed in the service sector. Each of the waves was initiated two months after the first wave. Finally, 286 useful responses were obtained. The collected data was analyzed through covariance-based structural equation modeling.

Findings
Servant leadership is related with meaning, promotive voice behavior and prohibitive voice behavior. Meaning is found to mediate the relationship between servant leadership and the two facets of voice behavior.

Originality/value
To the best of the authors’ knowledge, this study is the first to explore the mediating role of meaning relating servant leadership to promotive and prohibitive voice behavior.
Rethinking the role of hospitality in society: the HOST model
Authors: Bilgihan Anil , Hanks Lydia , Line Nathan Discepoli , Mody Makarand Amrish 
Published: September 2023

Purpose
The purpose of this conceptual paper is to provide a critical reflection on the role of hospitality in society. Specifically, this research criticizes contemporary conceptualizations of hospitality in academic research and practice and suggests a reconceptualized approach for capturing the full potential of hospitality to elicit transformative social change.

Design/methodology/approach
This paper is based on a critical analysis of hospitality research and practice as reflected in the extant literature. A typological approach to conceptualization is used to develop a framework that views hospitality from three distinct epistemological pathways.

Findings
Hospitality has largely been conceptualized as an industry- or a business-level context in which economic activity takes place, a pathway referred to as application. This paper offers the hospitality-oriented society of tomorrow (HOST) framework, which urges researchers and practitioners to explore two additional pathways – infusion and transformation – through which hospitality can contribute to society. The nonrecursive relationships between these three pathways and the five pillars of sustainable development espoused by the United Nations 2030 Agenda are proposed to form the basis of future inquiry into the role of hospitality in society.

Practical implications
The HOST model provides a framework whereby stakeholders within and outside of the traditional contours of the hospitality industry can benefit from a broader conceptualization and implementation of the hospitality phenomenon.

Originality/value
The paper offers a thought-provoking assessment of the fundamental tenets of hospitality as an academic discipline and social phenomenon. It offers a unique framework that should inform the evolution of hospitality research and practice if the discipline is to bolster its social significance.
Drivers of Nigeria’s real house prices: long-run analysis and short-run dynamics
Authors: Okey Paul Chinedu 
Published: September 2023

Purpose
The purpose of this paper is to assess the long-run and short-run drivers of real house prices in Nigeria from 1991Q1 to 2020Q4.

Design/methodology/approach
Vector autoregression and cointegration tests were used to assess the key drivers of Nigeria’s real house prices in the long run and short run.

Findings
The empirical findings revealed that household disposable income is the most important determinant of house prices in Nigeria. House prices increased by 1.6% and 60.8% in response to a 1% increase in disposable income in the long run and short run, respectively, while real mortgage credits pushed up house prices by 5% and have no long-run effects, suggesting that most Nigerians depend on their money income rather than credits in securing a home. In addition, prices of oil sector products and real interest rates had negative and significant relationship with house prices, while positive correlations were found for real effective exchange rate and real housing investments regardless of the time horizon. The impact of construction costs and cement prices was also documented.

Originality/value
This is likely a pioneering study of its kind to focus on the determinants of real house prices in Nigeria. It is probably the first study, the best of the author’s knowledge, to empirically examine the impact of the oil sector on house prices in the country.
Public information sharing in enterprise social networks: a communication privacy management perspective
Authors: Wang Yu , Zheng Daqing , Fang Yulin 
Published: September 2023

PurposeThe advancement of enterprise social networks (ESNs) facilitates information sharing but also presents the challenge of managing information boundaries. This study aims to explore the factors that influence the information-control behavior of ESN users when continuously sharing information.Design/methodology/approachThis study specifies the information-control behaviors in the “wall posts” channel and applies communication privacy management (CPM) theory to analyze the effects of the individual-specific factor (disposition to value information), context-specific factors (work-relatedness and information richness) and risk-benefit ratio (public benefit and public risk). Data on actual information-control behaviors extracted from ESN logs are examined using multilevel mixed-effects logistic regression analysis.FindingsThe study's findings show the direct effects of the individual-specific factor, context-specific factors and risk-benefit ratio, highlighting interactions between the individual motivation factor and ESN context factors.Originality/valueThis study reshapes the relationship of CPM theory boundary rules in the ESN context, extending information-control research and providing insights into ESNs' information-control practices.
How enterprise social media affordances affect employee agility: a self-determination theory perspective
Authors: Sun Yuan , Mengyi Zhu , Jeyaraj Anand 
Published: September 2023

PurposeThis paper aims to investigate whether and how enterprise social media (ESM) affordances affect employee agility.Design/methodology/approachAdopting self-determination theory (SDT), this study examines a model in which the four ESM affordances (i.e. visibility, association, editability and persistence) impact employee agility through the three basic psychological needs satisfaction (i.e. perceived autonomy, perceived relatedness and perceived competence) of employees. Mplus 7.4 was used to analyze survey data gathered from 304 employees who used ESM in the workplace.FindingsThe authors’ findings show that all four ESM affordances contribute to perceived relatedness and perceived competence; visibility and association affordances also have positive impacts on perceived autonomy; and all three psychological needs satisfaction positively impact employee agility.Originality/valueFirst, this study adapted SDT to explore how ESM influences employee agility. Second, this study enriches the relevant research on the antecedents of employee agility and also provides new evidence and theoretical support for employee agility. Third, this study effectively expands the antecedents and outcomes of employee basic psychological needs satisfaction in the domain of ESM and agility.
Effect of creative and collaborative learning communities on virtual learning environment for Education 4.0: a quantitative study of Pakistan
Authors: Asad Muhammad Mujtaba , Hussain Safdar 
Published: September 2023

PurposeEducation 4.0 is one of the major transformations in the field of education to improve the quality of teaching and learning processes and prepare students as 21st-century learners. This study aims to examine the effect of Educations 4.0 on creative and collaborative learning of students.Design/methodology/approachIn this correlational research study, a quantitative data collection tool such as a questionnaire is used. This research is conducted on university students (N = 365), and purposive sampling technique is used to achieve the purpose. Statistical Package for Social Sciences (SPSS) version 27.0 is used to analyze the data.FindingsThe findings of the study reveal that there is a strong positive relationship between education 4.0 and the two major 21st-century skills, i.e. collaboration and creativity. The value of correlation coefficient value of the variables is 0.597, which indicates positive and strong relationship; hence, null hypothesis is rejected and an alternative hypothesis is accepted.Practical implicationsThe results of the current study are beneficial for the school managers to enhance students' two significant 21st-century skills, i.e. “Creativity” and “Collaboration.” Also, the school managers can make efforts to develop teachers' competence to adapt the technological advancements for improving students' creativity and collaboration to benefit their learning.Originality/valueThis is a unique study because there are only a few recent studies that show a connection between Education 4.0 and 21st-century skills. Also, there is no other study that presents the direct association of Education 4.0 and students' creativity and collaboration specifically in the context of Pakistan.
Navigating services for consumers with disabilities: a comprehensive review and conceptual framework
Authors: Tuli Nikhita , Srivastava Ritu , Kumar Harish 
Published: September 2023

PurposeAccess to services for consumers with disabilities (CwD) has gained increased attention from researchers and service providers in recent years. Consequently, ensuring that services are designed and maintained in a manner that is more inclusive and accessible to CwD has become imperative. However, academic literature is fragmented and thus, this study aims to provide a state-of-the-art synthesis for further theoretical development.Design/methodology/approachThis study reviews 77 relevant articles in the domain using a multidisciplinary review following the PRISMA protocol, and a thematic analysis was conducted.FindingsThe study thoroughly synthesizes the theories, contexts and methods used in the extant literature. Next, the study presents a new theoretical framework with four broader dimensions: beyond regulations, towards accessibility, value co-creation, inclusion of CwD and role of stakeholders. Furthermore, it highlights the related sub-dimensions attributed to the service design stages (planning, usage and post-usage). Based on this, the study offers critical avenues for future research using the Double Diamond framework.Originality/valueThe study contributes significantly to service design literature for CwD and transformative service research by developing a new consolidated theoretical framework. The findings should direct service providers towards better service designs in related fields. Socially, the study has implications for promoting accessibility and inclusion for CwD, while providing them the freedom of choice.
Workplace learning in transient workplaces: the tourism and hospitality industry in the Arctic region
Authors: Parding Karolina , Ek Styvén Maria , Lindström Frida , Näppä Anna 
Published: September 2023

Purpose
This paper aims to focus on conditions for workplace learning (WPL) in highly transient workplaces, exemplified by the tourism and hospitality sector in the Arctic region. The aim is to analyse and discuss how employees and employers view the conditions for employees’ WPL from their respective perspectives.

Design/methodology/approach
The study is based on a qualitative approach. Ten interviews with employers and ten interviews with employees were carried out. This opens for different perspectives, including identifying “learning gaps”. The analysis was thematic, with a focus on opportunities and challenges for WPL in these transient workplace contexts.

Findings
Overall, conditions for WPL seem unsatisfactory. On the one hand, both employees and employers see WPL as essential for staff retention. Employers also see WPL as a strategy for business development and, thus, profit. On the other hand, high staff turnover makes it challenging to strategically invest in and organize for WPL, especially formal learning. Hence, a Catch-22 situation emerges.

Research limitations/implications
As this study is qualitative in its scope, generalizations are analytical rather than statistical.

Originality/value
There is a shortage of studies on conditions for WPL, focusing particularly on transient workplaces. Moreover, by including employer and employee perspectives, the authors contribute to a gap in the literature. The empirical contribution of this paper thus lies in using a theoretical WPL framework on transient workplaces, exemplified by the tourism and hospitality industries in the Arctic region.
Brand hate in the cosmetic industry: drugstore versus luxury make-up brands
Authors: Xin Ying Ong Sharon , Vila-Lopez Natalia 
Published: September 2023

Purpose
Marketing scholars have begun to look at negative emotions that young consumers could develop toward brands, such as brand hate. Brand hate has experienced exponential growth during the past decades in the cosmetic industry. In this frame, the purpose of this paper is to identify the weight of each of the five key drivers of brand hate and to analyze if these weights are the same (or not) for drugstore and luxury make-up brands regarding.

Design/methodology/approach
To carry on this paper, brand haters in the make-up industry were contacted with the help of cosmetic influencers. Participants of the online questionnaire (N = 162) were existing young makeup consumers. They were divided into drugstore and luxury makeup brand haters by classifying their identified hated brands into either group.

Findings
The authors’ results showed, first, that experiential, identity, moral, deficit-value and advertising avoidance all had a positive effect on brand hate, being identity avoidance the strongest one. Second, drugstore and luxury makeup brand haters do not differ, as far as no differences were identified in the strength of each avoidance type on brand hate.

Originality/value
There is a gap in the literature related to the absence of work investigating brand hate in the make-up industry; moreover, studies measure whether brand hate drivers are the same (or not) for luxury brands and drugstore brands that compete in the same arena. In this framework, this research will provide a specific industry context involving young consumer opinions. Research into consumer–brand relationships has been largely focused on the positive forms, while the negative forms are still a relatively newer area of academic interest. Even more, brand hate has been investigated from a multidimensional approach linking proposals from different authors has been tested.
Servant Leadership as a Catalyst for Middle Managers’ Learning Ambidexterity: A Resource‐Based Perspective
Authors: Usman Muhammad , Ogbonnaya Chidiebere , Roodbari Hamid , Yusuf Rebecca , Hirekhan Manjusha 
Published: September 2023

AbstractAlthough recent years have seen a proliferation of research on organizational ambidexterity, important questions remain about the role that leaders play in leveraging learning ambidexterity for organizational benefits. Drawing on the conservation of resources theory, we investigate the indirect links between servant leadership and middle managers’ learning ambidexterity, with structural empowerment and role breadth self‐efficacy (RBSE) as serial mediators. We also examine the importance of leader boundary‐spanning behaviour as a moderating factor for these relationships. Using time‐lagged and multi‐source data from 344 middle managers and their supervisors, we show that servant leadership has a positive indirect influence on two forms of learning ambidexterity: exploitative and explorative learning. In particular, servant leadership promotes structural empowerment (as a contextual resource), which in turn influences RBSE (as a personal resource) and encourages learning ambidexterity. In addition, we show that when leaders engage in boundary‐spanning behaviour, these indirect relationships become more prominent. This research offers new theoretical and practical insights to assist organizations in improving learning ambidexterity and achieving higher levels of performance.
Unveiling changes in rhizosphere‐associated bacteria linked to the genotype and water stress in quinoa
Authors: Maestro‐Gaitán Isaac , Granado‐Rodríguez Sara , Redondo‐Nieto Miguel , Battaglia Antonio , Poza‐Viejo Laura , Matías Javier , Bolaños Luis , Reguera Maria 
Published: September 2023

AbstractDrought is among the main abiotic factors causing agronomical losses worldwide. To minimize its impact, several strategies have been proposed, including the use of plant growth‐promoting bacteria (PGPBs), as they have demonstrated roles in counteracting abiotic stress. This aspect has been little explored in emergent crops such as quinoa, which has the potential to contribute to reducing food insecurity. Thus, here we hypothesize that the genotype, water environment and the type of inoculant are determining factors in shaping quinoa rhizosphere bacterial communities, affecting plant performance. To address this, two different quinoa cultivars (with contrasting water stress tolerance), two water conditions (optimal and limiting water conditions) and different soil infusions were used to define the relevance of these factors. Different bacterial families that vary among genotypes and water conditions were identified. Certain families were enriched under water stress conditions, such as the Nocardioidaceae, highly present in the water‐sensitive cultivar F15, or the Pseudomonadaceae, Burkholderiaceae and Sphingomonadaceae, more abundant in the tolerant cultivar F16, which also showed larger total polyphenol content. These changes demonstrate that the genotype and environment highly contribute to shaping the root‐inhabiting bacteria in quinoa, and they suggest that this plant species is a great source of PGPBs for utilization under water‐liming conditions.
Landmarks in the history of selective sweeps
Authors: Panigrahi Manjit , Rajawat Divya , Nayak Sonali Sonejita , Ghildiyal Kanika , Sharma Anurodh , Jain Karan , Lei Chuzhao , Bhushan Bharat , Mishra Bishnu Prasad , Dutt Triveni 
Published: September 2023

AbstractHalf a century ago, a seminal article on the hitchhiking effect by Smith and Haigh inaugurated the concept of the selection signature. Selective sweeps are characterised by the rapid spread of an advantageous genetic variant through a population and hence play an important role in shaping evolution and research on genetic diversity. The process by which a beneficial allele arises and becomes fixed in a population, leading to a increase in the frequency of other linked alleles, is known as genetic hitchhiking or genetic draft. Kimura's neutral theory and hitchhiking theory are complementary, with Kimura's neutral evolution as the ‘null model’ and positive selection as the ‘signal’. Both are widely accepted in evolution, especially with genomics enabling precise measurements. Significant advances in genomic technologies, such as next‐generation sequencing, high‐density SNP arrays and powerful bioinformatics tools, have made it possible to systematically investigate selection signatures in a variety of species. Although the history of selection signatures is relatively recent, progress has been made in the last two decades, owing to the increasing availability of large‐scale genomic data and the development of computational methods. In this review, we embark on a journey through the history of research on selective sweeps, ranging from early theoretical work to recent empirical studies that utilise genomic data.