bluuebunny/crossref_metadata_2025_split huggingface dataset:
In Search of a Lost Childhood
Authors: Moreh-Rosenberg Eliad 
Published: March 2023

Abstract
Sigalit Landau is an internationally recognised artist born in Jerusalem whose works have been exhibited in major museums worldwide. This article seeks to analyse the sculpture Father and Tufik (2014), in which the artist relates for the first time to the story of her father, a Holocaust survivor from Transnistria. Starting with the creative process that led to the sculpture and examining unpublished sketches, the study shows how the work involves a multigenerational tale and a desire for communication and transmission which is further developed in a series of large-sized sculptures. Furthermore, elements of play, which Ernst van Alphen identifies as a means used by younger generations of artists to confront Holocaust remembrance, can be found in this sculpture and in other key works including Barbed Hula (2000) and One Man's Floor Is Another Man's Feelings (2011) which can thus be interpreted in the context of the Shoah.
CURRENT PROFESSIONAL AND PEDAGOGICAL REQUIREMENTS FOR EDUCATORS OF HIGHER MEDICAL EDUCATIONAL INSTITUTION
Authors: Nіemtchenko І.І. , Liakhovskyi V.І. , Lysenko R.B. , Liulka O.N. , Krasnov O. G. , Ryabushko R.M. , Horodova-Andryeyeva T.V. 
Published: March 2023

The primary role of educators at a higher medical educational institution involves sharing knowledge, providing educational guidance, and ensuring students understand the subjects they teach. This mission requires special mental traits, long-life training, ability to combine the knowledge and erudition of a scientist and the art of a teacher, high intellectual and moral maturity, awareness of the duty and responsibility for fostering and training highly qualified medical specialists.
Therefore, the professional pedagogical activity of educators must be considered as a complete dynamic system that combines the theoretical components related to providing the essence of science and practical components aimed at solving specific practical tasks.
The article considers the modern requirements for educators of a higher medical educational institution, determines the complex of the personal, professional and pedagogical characteristics. The main functions of the pedagogical activity of a modern educator of a higher medical educational institution over the course of doctor’s training are supported by various innovative teaching and learning technologies aimed at strengthening the motivation to study, ability to work independently, fostering clinical thinking, professional skills and abilities. This enables to achieve new results in the formation of the student's personal qualities, creating a basis for the further development of a creative personality, their preparation for future independent medical activity as well as the awareness of the need for lifelong education.
ASSESSMENT OF EFFECTIVENESS IN APPLYING MULTIDISCIPLINARY EXPERIMENTALLY-BASED APPROACH IN CHOOSING THERAPY FOR PATIENTS WITH FUNCTIONAL MAXILLOFACIAL DISORDERS IN COMBINATION WITH PRIMARY DEEP BITE
Authors: Flis P.S. , Holubchenko O.Yu. 
Published: March 2023

Introduction. In recent years, the percentage of adults seeking orthodontic care has been increasing. The management of such a group of patients poses a number of challenges the orthodontists have to solve. One of the common problems that arise during the bite correction with fixed orthodontic appliances is debonding. The highest percentage, 43%, of adhesion disruption is observed in patients with a deep overlap. There is also an increase in the number of dental patients presenting with manifestations of the development of functional occlusion disorders caused by reconstructive prosthetic restorations under the existing bite pathology.
Materials and methods. A comparison of electromyography indicators in patients before and after the treatment was carried out. The study included 51 patients aged 18-45 years (average age was 29.6±6.1 years). The selection was made on the basis of inclusion / exclusion criteria. The first group involved 24 patients (9 men, 15 women) with a deep bite, occlusal disorders with unexpressed architecture of the occlusal surface of reconstructive restorations mainly made of composite and polymer materials; the participants presented no pronounced pain symptoms, but presented complaints about the masticatory muscles (discomfort, trismus, contracture of the lateral pterygoid muscle, bruxism, etc.). Patients of this group were characterized by multiple direct restorations and short-length orthopaedic structures. The second group included 27 patients (10 men, 17 women) with a deep bite, occlusal disorders, pronounced architecture of the occlusal surface of reconstructive prosthetic restorations and existing pain manifestations from the masticatory muscles, which appeared after their functional overload. In order to compare the effectiveness of treatment, each of the clinical groups was divided into 2 subgroups, test and control.
Results. The findings obtained demonstrated the normalization of bioelectric activity indicators after the treatment, both in the control group and in the test group (p<0.05). For patients of clinical group 1, the level of the bioelectrical activity after the treatment in the test group was statistically significantly (p=0.0049) lower than in the control group. The same was observed for the patients from 2 clinical groups (p<0.001)
Conclusions. The scheme of the proposed algorithm for the fixation of orthodontic appliances and measures aimed at normalizing the work of masticatory muscles significantly improves the indicators of functional research methods, in particular, the indicators of bioelectric activity during electromyography in patients of all study groups.
EFFECT OF ENTERAL NUTRITION IN COMBINATION WITH L-ARGININE AND GLUTAMIC ACID ON NUTRITIONAL STATUS IN EXPERIMENT
Authors: Kushta A.A. , Melnyk A. V. , Voloshchuk N. I. 
Published: March 2023

The aim of the study is to evaluate the scheme of complex therapy based on enteral nutrition and L-arginine, glutamic acid on the indicators of protein, carbohydrate, and lipid metabolism in the blood of rats with experimental wound defects of the skin under conditions of partial food deprivation.
Materials and methods. A biochemical study of blood was carried out in 24 sexually mature male rats, which were partially food deprived for 10 days. On the 10th day, a wound was made and the animals were divided into 4 groups: the 1st group of control animals, which received food according to physiological needs; the 2nd group, which continued to be partially food deprived; and the 3rd group of rats, which after being exposed to dystrophy modelling for 10 days, were given compensatory enteral nutrition; the 4th group, which after the food deprivation, received L-arginine and glutamic acid in a dose of 50 mg/kg in addition to enteral nutrition. Blood sampling was carried out by removing the animals from the experiment on the 28th day. The following biochemical indicators were studied: the content of glucose, total protein, albumins, urea, the level of non-esterified fatty acids (NFA), ketone bodies, ammonia and TBC-reactants.
Results. Comparative analysis of glucose content showed that integrative therapy prevented the development of hypoglycemia under the conditions of partial food derivation (р˂0.05). The positive effect of the combined action of enteral nutrition and L-arginine, glutamic acid on the serum content of total protein was found to be higher by 18% (р˂0.05), and albumin by 39% (р˂0.05), urea and ammonia was by 50% and 40.1% smaller (р˂0.05), NEJK was lower by 32.4% (р˂0.05), the content of ketone bodies was lower by 73.2% (р˂0.05), and TBK- reactants was lower by 50.4% (р˂0.05), compared to the untreated animals, and these indicators did not statistically significantly differ from the control values.
Conclusion. The integrated therapy based on enteral nutrition and L-arginine, glutamic acid under the partial food deprivation effectively normalized protein metabolism, indicators of lipid and nitrogen metabolism in the body of rats (p˂0.05), relative to untreated animals.
Breaking the Ice: Young Feminist Scholars of Reproductive Politics Reflect on Egg Freezing
Authors: Hammond Kathleen , Cattapan Alana , Haw Jennie , Tarasoff Lesley A. 
Published: March 2023

<p>While proponents of social (i.e., nonmedical) egg freezing argue that it is liberating for women, opponents contest that the technology provides an individualist solution to a social problem. This article comprises personal and academic reflections on the debate on social egg freezing from four young women studying reproductive technologies. We challenge the promotion of social egg freezing as an empowering option for women and question cultural assumptions about childbearing, the disclosure of risk, failures to consider sexual diversity and socioeconomic status, and the expansion of the market in reproductive tissues. </p>
Governments’ accountability for Canada’s pandemic response
Authors: Khoury Lara , Klein Alana , Couture-Ménard Marie-Eve , Hammond Kathleen 
Published: March 2023

<p>The COVID-19 pandemic—with its wide-reaching social, political, and economic implications—showcases the importance of public health governance. Governmental accountability is at the forefront of societal preoccupations, as state actors attempt to manage the pandemic by using sweeping emergency powers which grant them significant discretion. Though emergency measures have tremendous impacts on citizens’ lives, elected officials and civil society have little input in how governments wield these powers. We reviewed available mechanisms in Canadian private, constitutional, and criminal law and found them to be unlikely sources of much-needed accountability. Therefore, we propose that provincial and territorial legislatures modify public health legislation to expand mechanisms to foster public confidence in decision-makers, and bolster accountability to parliaments and citizens.</p>
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Montreal’s Sanctuary Policy for Non-Status Migrants: Opportunity and Obstacles
Authors: Atak Idil , Hudson Graham 
Published: March 2023

<p>In February 2017, the city of Montreal adopted a policy entitled <strong>Access to Municipal Services Without Fear</strong> with a view to allowing non-status migrants to access some municipal programs and services without fear of being arrested and removed from Canada. This article offers a critical analysis of the city of Montreal’s policy. We discuss the main barriers to implementation, namely, the limited jurisdictional power and authority of the city in migration matters and the municipal police’s refusal to comply with the policy. We draw on the conceptual framework of the sanctuary city to argue that the municipality crafted creative solutions to barriers as part of its bid to make the city more inclusive.</p>
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Hepatitis C virus fitness can influence the extent of infection-mediated epigenetic modifications in the host cells
Authors: García-Crespo Carlos , Francisco-Recuero Irene , Gallego Isabel , Camblor-Murube Marina , Soria María Eugenia , López-López Ana , de Ávila Ana Isabel , Madejón Antonio , García-Samaniego Javier , Domingo Esteban , Sánchez-Pacheco Aurora , Perales Celia 
Published: March 2023

IntroductionCellular epigenetic modifications occur in the course of viral infections. We previously documented that hepatitis C virus (HCV) infection of human hepatoma Huh-7.5 cells results in a core protein-mediated decrease of Aurora kinase B (AURKB) activity and phosphorylation of Serine 10 in histone H3 (H3Ser10ph) levels, with an affectation of inflammatory pathways. The possible role of HCV fitness in infection-derived cellular epigenetic modifications is not known.MethodsHere we approach this question using HCV populations that display a 2.3-fold increase in general fitness (infectious progeny production), and up to 45-fold increase of the exponential phase of intracellular viral growth rate, relative to the parental HCV population.ResultsWe show that infection resulted in a HCV fitness-dependent, average decrease of the levels of H3Ser10ph, AURKB, and histone H4 tri-methylated at Lysine 20 (H4K20m3) in the infected cell population. Remarkably, the decrease of H4K20m3, which is a hallmark of cellular transformation, was significant upon infection with high fitness HCV but not upon infection with basal fitness virus.DiscussionHere we propose two mechanisms ─which are not mutually exclusive─ to explain the effect of high viral fitness: an early advance in the number of infected cells, or larger number of replicating RNA molecules per cell. The implications of introducing HCV fitness as an influence in virus-host interactions, and for the course of liver disease, are warranted. Emphasis is made in the possibility that HCV-mediated hepatocellular carcinoma may be favoured by prolonged HCV infection of a human liver, a situation in which viral fitness is likely to increase.
Meta-analysis and systematic review of coronary vasospasm in ANOCA patients: Prevalence, clinical features and prognosis
Authors: Woudstra Janneke , Vink Caitlin E. M. , Schipaanboord Diantha J. M. , Eringa Etto C. , den Ruijter Hester M. , Feenstra Rutger G. T. , Boerhout Coen K. M. , Beijk Marcel A. M. , de Waard Guus A. , Ong Peter , Seitz Andreas , Sechtem Udo , Piek Jan J. , van de Hoef Tim P. , Appelman Yolande 
Published: March 2023

BackgroundCoronary artery spasm (CAS), encompassing epicardial and microvascular spasm, is increasingly recognized as cause of angina in patients with non-obstructive coronary artery disease (ANOCA). However, various spasm provocation testing protocols and diagnostic criteria are used, making diagnosis and characterization of these patients difficult and interpretation of study results cumbersome. This review provides a structured overview of the prevalence, characterization and prognosis of CAS worldwide in men and women.MethodsA systematic review identifying studies describing ANOCA patients with CAS was performed. Multiple outcomes (prevalence, clinical features, and prognosis) were assessed. Data, except for prognosis were pooled and analysed using random effects meta-analysis models.ResultsTwenty-five publications (N = 14.554) were included (58.2 years; 44.2% women). Percentages of epicardial constriction to define epicardial spasm ranged from &gt;50% to &gt;90%. Epicardial spasm was prevalent in 43% (range 16–73%), with a higher prevalence in Asian vs. Western World population (52% vs. 33%, p = 0.014). Microvascular spasm was prevalent in 25% (range 7–39%). Men were more likely to have epicardial spasm (61%), women were more likely to have microvascular spasm (64%). Recurrent angina is frequently reported during follow-up ranging from 10 to 53%.ConclusionCAS is highly prevalent in ANOCA patients, where men more often have epicardial spasm, women more often have microvascular spasm. A higher prevalence of epicardial spasm is demonstrated in the Asian population compared to the Western World. The prevalence of CAS is high, emphasizing the use of unambiguous study protocols and diagnostic criteria and highlights the importance of routine evaluation of CAS in men and women with ANOCA.Systematic Review Registrationhttps://www.crd.york.ac.uk/prospero/display_record.php?RecordID=272100.
Lack of association between leptin concentrations and cystic fibrosis: A meta-analysis and regression
Authors: Qi Hong , Liu Hairong , Zheng Pengcheng , He Jie 
Published: March 2023

BackgroundLeptin (LEP) acts as a proinflammatory cytokine and may play an important role in the pathophysiology of cystic fibrosis (CF). This review aimed to assess the quantitative difference in leptin status between CF patients and non-CF controls.MethodsIn this study, the researchers conducted systematic searches of various databases, such as PubMed, Excerpta Medica Database, Google Scholar, Web of Science, and the China National Knowledge Infrastructure. The data collected from the above databases were assessed using the Stata 11.0 and R 4.1.3 software. The correlation coefficients and the Standardized Mean Differences (SMD) were employed to assess the effect size. A combination analysis was also carried out with the help of either a fixed-effects or random-effects model. In addition, the single-cell sequencing GSE193782 dataset was obtained to determine the mRNA expression levels of LEP and leptin receptor (LEPR) in the bronchoalveolar lavage fluid, to verify the different leptin expression between the CF patients and healthy controls.ResultsA total of 919 CF patients and 397 controls from 14 articles were included in this study. CF patients and non-CF controls showed similar serum/plasma leptin levels. Gender, specimen testing, age, and study design were all taken into account for carrying out subgroup analyses. The results revealed no variations in serum/plasma leptin levels between the controls and CF patients in the various subgroups. Female CF patients exhibited higher leptin concentrations compared to male CF patients, and male healthy individuals showed lower leptin levels than female healthy participants. Aside from the fact that serum/plasma leptin appeared to be favorably linked to fat mass and BMI, the findings in this study also indicated that serum/plasma concentrations were not associated with Forced Expiratory Volume in the first second (FEV1). No statistically significant differences were observed in the leptin and leptin receptor mRNA expression levels between the healthy controls and CF patients. The leptin receptor and leptin expression levels in alveolar lavage fluid were low in various cells, without any distinctive distribution patterns.ConclusionsThe current meta-analysis indicated the absence of significant differences in leptin levels between CF patients and healthy individuals. Gender, fat mass, and BMI may all be correlated with leptin concentrations.Systematic review registrationhttps://www.crd.york.ac.uk/prospero/, identifier CRD42022380118.
Proteomic characterisation of perhexiline treatment on THP-1 M1 macrophage differentiation
Authors: Dhakal Bimala , Li Celine Man Ying , Ramezanpour Mahnaz , Houtak Ghais , Li Runhao , Bouras George , Collela Alex , Chegeni Nusha , Chataway Tim Kennion , Drew Paul , Sallustio Benedetta C. , Vreugde Sarah , Smith Eric , Maddern Guy , Licari Giovanni , Fenix Kevin 
Published: March 2023

BackgroundDysregulated inflammation is important in the pathogenesis of many diseases including cancer, allergy, and autoimmunity. Macrophage activation and polarisation are commonly involved in the initiation, maintenance and resolution of inflammation. Perhexiline (PHX), an antianginal drug, has been suggested to modulate macrophage function, but the molecular effects of PHX on macrophages are unknown. In this study we investigated the effect of PHX treatment on macrophage activation and polarization and reveal the underlying proteomic changes induced.MethodsWe used an established protocol to differentiate human THP-1 monocytes into M1 or M2 macrophages involving three distinct, sequential stages (priming, rest, and differentiation). We examined the effect of PHX treatment at each stage on the polarization into either M1 or M2 macrophages using flow cytometry, quantitative polymerase chain reaction (qPCR) and enzyme linked immunosorbent assay (ELISA). Quantitative changes in the proteome were investigated using data independent acquisition mass spectrometry (DIA MS).ResultsPHX treatment promoted M1 macrophage polarization, including increased STAT1 and CCL2 expression and IL-1β secretion. This effect occurred when PHX was added at the differentiation stage of the M1 cultures. Proteomic profiling of PHX treated M1 cultures identified changes in metabolic (fatty acid metabolism, cholesterol homeostasis and oxidative phosphorylation) and immune signalling (Receptor Tyrosine Kinase, Rho GTPase and interferon) pathways.ConclusionThis is the first study to report on the action of PHX on THP-1 macrophage polarization and the associated changes in the proteome of these cells.
Development of multi-epitope vaccines against the monkeypox virus based on envelope proteins using immunoinformatics approaches
Authors: Tan Caixia , Zhu Fei , Pan Pinhua , Wu Anhua , Li Chunhui 
Published: March 2023

BackgroundSince May 2022, cases of monkeypox, a zoonotic disease caused by the monkeypox virus (MPXV), have been increasingly reported worldwide. There are, however, no proven therapies or vaccines available for monkeypox. In this study, several multi-epitope vaccines were designed against the MPXV using immunoinformatics approaches.MethodsThree target proteins, A35R and B6R, enveloped virion (EV) form-derived antigens, and H3L, expressed on the mature virion (MV) form, were selected for epitope identification. The shortlisted epitopes were fused with appropriate adjuvants and linkers to vaccine candidates. The biophysical andbiochemical features of vaccine candidates were evaluated. The Molecular docking and molecular dynamics(MD) simulation were run to understand the binding mode and binding stability between the vaccines and Toll-like receptors (TLRs) and major histocompatibility complexes (MHCs). The immunogenicity of the designed vaccines was evaluated via immune simulation.ResultsFive vaccine constructs (MPXV-1-5) were formed. After the evaluation of various immunological and physicochemical parameters, MPXV-2 and MPXV-5 were selected for further analysis. The results of molecular docking showed that the MPXV-2 and MPXV-5 had a stronger affinity to TLRs (TLR2 and TLR4) and MHC (HLA-A*02:01 and HLA-DRB1*02:01) molecules, and the analyses of molecular dynamics (MD) simulation have further confirmed the strong binding stability of MPXV-2 and MPXV-5 with TLRs and MHC molecules. The results of the immune simulation indicated that both MPXV-2 and MPXV-5 could effectively induce robust protective immune responses in the human body.ConclusionThe MPXV-2 and MPXV-5 have good efficacy against the MPXV in theory, but further studies are required to validate their safety and efficacy.
Characteristics of auditory brainstem response in wild yellow grouper Epinephelus awoara
Authors: Zhu Xiangyu , Zhang Xinhai , Chen Mingru , Xu Xiaomei 
Published: March 2023

Auditory brainstem response (ABR) technology is used to set up a fish auditory threshold detection system, and the auditory threshold of wild yellow grouper Epinephelus awoara caught in the Taiwan Strait is studied. The ABRs of 10 wild yellow groupers (TL150-200 mm) to 12 different frequencies of sound stimulation from 100 to 2000 Hz are recorded and analyzed. The results show that the auditory threshold of wild yellow grouper fish increases with the increase of frequency in the frequency of 200-2000Hz. The fish is sensitive to low frequency sound signal of 100-300Hz with the most sensitive frequency of 200Hz, and the auditory threshold is 90 dB re 1μPa. The ABR waveform shows three periods: response period, real hearing period and extension period. In the range of 100-2000Hz, the response time decreases with the increase of frequency at the same sound pressure level. At the same frequency, the response time increases with the decrease of sound pressure level. The first amplitude sound pressure of real hearing period is between 50-150nv, and the real hearing period contains the greatest amplitude. When the greatest amplitude is about 50nv and the next sound pressure level is below 50nv, the sound pressure level is the auditory threshold. The extension period is proportional to the response period.