bluuebunny/crossref_metadata_2025_split huggingface dataset:
Ductus arteriosus morphology in duct-dependent pulmonary circulation: CT classification and pattern in different ventricular morphology
Authors: Abdul Latiff Haifa , Gopal Anu Ratha , Hidayat Zul Febrianti , Haranal Maruti , Borhanuddin Boekren K. , Alwi Mazeni , Samion Hasri 
Published: January 2023

AbstractBackground:The objective was to study the ductus arteriosus morphology in duct-dependent pulmonary circulation and its pattern in different ventricle morphology using CT angiography.Method:From January 2013 to December 2015, patients aged 6 months and below with duct-dependent pulmonary circulation underwent CT angiography to delineate the ductus arteriosus origin, tortuosity, site of insertion, and pulmonary artery anatomy. The ductus arteriosus were classified into type I, IIa, IIb, and III based on its site of origin, either from descending aorta, distal arch, proximal arch, or subclavian artery, respectively.Results:A total of 114 patients and 116 ductus arteriosus (two had bilateral ductus arteriosus) were analysed. Type I, IIa, IIb, and III ductus arteriosus were seen in 13 (11.2 %), 71 (61.2%), 21 (18.1%), and 11 (9.5%), respectively. Tortuous ductus arteriosus was found in 38 (32.7%), which was commonly seen in single ventricular lesions. Ipsilateral and bilateral branch pulmonary artery stenosis was seen in 68 (59.6%) and 6 (5.3%) patients, respectively. The majority of patients with pulmonary atresia intact ventricular septum had type I (54.4%) and non-tortuous ductus arteriosus, while those with single and biventricular lesions had type II ductus arteriosus (84.9% and 89.7%, respectively). Type III ductus arteriosus was more common in biventricular lesions (77.8%).Conclusions:Ductus arteriosus in duct-dependent pulmonary circulation has a diverse morphology with a distinct origin and tortuosity pattern in different types of ventricular morphology. CT may serve as an important tool in case selection and pre-procedural planning for ductal stenting.
Satisfaction With Life Among Vaccinated Turkish People
Authors: Geçer Ekmel , Yıldırım Murat , Akgül Ömer , Sögütlü Lütfiye , Akif Sezerol Mehmet , Tașçı Yusuf 
Published: January 2023

Abstract. Introduction: Past research has shown that psychological and behavioral factors, including anxiety, fear, and preventive behaviors, are related to well-being outcomes during the COVID-19 pandemic. Yet little is known about the simultaneous effects of those factors in predicting satisfaction with life among vaccinated people. Methods: This cross-sectional study is among the first to examine whether COVID-19 anxiety, fear of COVID-19, and preventive behaviors predicted satisfaction with life among the vaccinated general public in Turkey. Participants were 1,017 Turkish adults (69.71% female; mean age = 36.28, SD = 10.83 years) who completed online self-reported questionnaires. Results: Results showed that increased levels of anxiety and fear of COVID-19 significantly predicted reduced satisfaction with life over and above the effects of sociodemographic and clinical factors. Discussion: The findings suggest the importance of tailoring and implementing interventions focusing on the improvement of the well-being of people during the pandemic.
AMOC Stabilization Under the Interaction With Tipping Polar Ice Sheets
Authors: Sinet S. , von der Heydt A. S. , Dijkstra H. A. 
Published: January 2023

AbstractSeveral large‐scale components of the climate system may undergo a rapid transition as critical conditions are exceeded. These tipping elements are also dynamically coupled, allowing for a global domino effect under global warming. Here we focus on such cascading events involving the Greenland Ice Sheet (GIS), the West Antarctica Ice Sheet (WAIS) and the Atlantic Meridional Overturning Circulation (AMOC). Using a conceptual model, we study the combined tipping behavior due to three dominant feedbacks: the marine ice sheet instability for the WAIS, the height‐surface mass balance feedback for the GIS and the salt‐advection feedback for the AMOC. We show that, in a realistic parameter range of the model, a tipping of the WAIS can inhibit cascading events by preserving the AMOC stability.
Opposite Impacts of Interannual and Decadal Pacific Variability in the Extratropics
Authors: Seabrook M. , Smith D. M. , Dunstone N. J. , Eade R. , Hermanson L. , Scaife A. A. , Hardiman S. C. 
Published: January 2023

AbstractIt is well established that the positive phase of El Niño Southern Oscillation (ENSO) tends to weaken the Northern Hemisphere stratospheric polar vortex (SPV), promoting a negative North Atlantic Oscillation (NAO). Pacific Decadal Variability (PDV) is characterized by a pattern of sea surface temperatures similar to ENSO, but its impacts are more uncertain: some studies suggest similar impacts of ENSO and PDV on the SPV and NAO, while others find the opposite. We use climate model experiments and reanalysis to find further evidence supporting opposite interannual and decadal impacts of Pacific variability on the extratropics. We propose that the decadal strengthening of the SPV in response to positive PDV is caused by a build‐up of stratospheric water vapor leading to enhanced cooling at the poles, an increased meridional temperature gradient and a strengthened extratropical jet. Our results are important for understanding decadal variability, seasonal to decadal forecasts and climate projections.
Toward High‐Resolution Global Atmospheric Inverse Modeling Using Graphics Accelerators
Authors: Chevallier Frédéric , Lloret Zoé , Cozic Anne , Takache Sakina , Remaud Marine 
Published: January 2023

AbstractThe ability of global transport models to go up in resolution becomes discriminating for greenhouse gas atmospheric inversions. This paper describes the porting on Graphics Processing Units of the global transport model currently used in the European operational Copernicus Atmosphere Monitoring Service (CAMS) for CO2 and N2O inversions. It represents an important milestone to achieve sub‐degree resolution. The code includes not only the direct model but also its tangent‐linear and its adjoint versions which are needed in variational inversions. Tests were carried out for CO2 at a resolution of 2.50° in longitude, 1.27° in latitude and 79 layers in the vertical, corresponding to 1,626,768 3D cells, 4.5 times more than the current standard resolution of the model used in the CAMS reanalyzes. A month's worth of computation of the tangent‐linear and of the adjoint versions now takes 2.5 min, including 50 s for reading meteorological data.
The Radiative and Cloud Responses to Sea Salt Aerosol Engineering in GFDL Models
Authors: Mahfouz Naser G. A. , Hill Spencer A. , Guo Huan , Ming Yi 
Published: January 2023

AbstractMarine cloud brightening is a proposal to counteract global warming by increasing sea salt aerosol emissions. In theory, this increases the cloud droplet number concentration of subtropical marine stratocumulus decks, increasing cloud brightness and longevity. However, this theoretical progression remains uncertain in coupled climate models, especially the response of liquid water path and cloud fraction to aerosol seeding. We use the GFDL CM4 climate model to simulate marine cloud brightening following the published G4sea‐salt protocol, in which sea salt aerosol emissions are uniformly increased over 30 S–30 N in addition to standard forcings from a SSP2‐4.5 future warming scenario. The perturbed radiative and cloud responses are temporally stable though spatially heterogeneous, and direct scattering by the added sea salt predominates over changes to cloud reflectance. In fact, feedbacks in the coupled simulation lead to a net warming, rather than cooling, response by clouds.
Romidepsin-CHOEP followed by high-dose chemotherapy and stem-cell transplantation in untreated Peripheral T-Cell Lymphoma: results of the PTCL13 phase Ib/II study
Authors: Chiappella Annalisa , Dodero Anna , Evangelista Andrea , Re Alessandro , Orsucci Lorella , Usai Sara Veronica , Castellino Claudia , Stefoni Vittorio , Pinto Antonio , Zanni Manuela , Ciancia Rosanna , Ghiggi Chiara , Rossi Francesca Gaia , Arcari Annalisa , Ilariucci Fiorella , Zilioli Vittorio Ruggero , Flenghi Leonardo , Celli Melania , Volpetti Stefano , Benedetti Fabio , Ballerini Filippo , Musuraca Gerardo , Bruna Riccardo , Patti Caterina , Leonardi Francesco , Arcaini Luca , Magagnoli Massimo , Cavallo Federica , Bermema Anisa , Tucci Alessandra , Boccomini Carola , Ciccone Giovannino , Carniti Cristiana , Pileri Stefano Aldo , Corradini Paolo 
Published: January 2023

AbstractThe standard treatment for young patients with untreated PTCLs is based on anthracycline containing-regimens followed by high-dose-chemotherapy and stem-cell-transplantation (HDT + SCT), but only 40% of them can be cured. Romidepsin, a histone-deacetylase inhibitor, showed promising activity in relapsed PTCLs; in first line, Romidepsin was added with CHOP. We designed a study combining romidepsin and CHOEP as induction before HDT + auto-SCT in untreated PTCLs (PTCL-NOS, AITL/THF, ALK-ALCL), aged 18–65 years. A phase Ib/II trial was conducted to define the maximum tolerated dose (MTD) of Ro-CHOEP, and to assess efficacy and safety of 6 Ro-CHOEP as induction before HDT. The study hypothesis was to achieve a 18-month PFS of 70%. Twenty-one patients were enrolled into phase Ib; 7 dose-limiting toxicities were observed, that led to define the MTD at 14 mg/ms. Eighty-six patients were included in the phase II. At a median follow-up of 28 months, the 18-month PFS was 46.2% (95%CI:35.0–56.7), and the 18-month overall survival was 73.1% (95%CI:61.6–81.7). The overall response after induction was 71%, with 62% CRs. No unexpected toxicities were reported. The primary endpoint was not met; therefore, the enrollment was stopped at a planned interim analysis. The addition of romidepsin to CHOEP did not improve the PFS of untreated PTCL patients.
Thermal acclimation of methanotrophs from the genusMethylobacter
Authors: Tveit Alexander T , Söllinger Andrea , Rainer Edda Marie , Didriksen Alena , Hestnes Anne Grethe , Motleleng Liabo , Hellinger Hans-Jörg , Rattei Thomas , Svenning Mette M 
Published: January 2023

AbstractMethanotrophs oxidize most of the methane (CH4) produced in natural and anthropogenic ecosystems. Often living close to soil surfaces, these microorganisms must frequently adjust to temperature change. While many environmental studies have addressed temperature effects on CH4 oxidation and methanotrophic communities, there is little knowledge about the physiological adjustments that underlie these effects. We have studied thermal acclimation in Methylobacter, a widespread, abundant, and environmentally important methanotrophic genus. Comparisons of growth and CH4 oxidation kinetics at different temperatures in three members of the genus demonstrate that temperature has a strong influence on how much CH4 is consumed to support growth at different CH4 concentrations. However, the temperature effect varies considerably between species, suggesting that how a methanotrophic community is composed influences the temperature effect on CH4 uptake. To understand thermal acclimation mechanisms widely we carried out a transcriptomics experiment with Methylobacter tundripaludum SV96T. We observed, at different temperatures, how varying abundances of transcripts for glycogen and protein biosynthesis relate to cellular glycogen and ribosome concentrations. Our data also demonstrated transcriptional adjustment of CH4 oxidation, oxidative phosphorylation, membrane fatty acid saturation, cell wall composition, and exopolysaccharides between temperatures. In addition, we observed differences in M. tundripaludum SV96T cell sizes at different temperatures. We conclude that thermal acclimation in Methylobacter results from transcriptional adjustment of central metabolism, protein biosynthesis, cell walls and storage. Acclimation leads to large shifts in CH4 consumption and growth efficiency, but with major differences between species. Thus, our study demonstrates that physiological adjustments to temperature change can substantially influence environmental CH4 uptake rates and that consideration of methanotroph physiology might be vital for accurate predictions of warming effects on CH4 emissions.
Acquired Pedophilia: international Delphi-method-based consensus guidelines
Authors: Scarpazza Cristina , Costa Cristiano , Battaglia Umberto , Berryessa Colleen , Bianchetti Maria Lucia , Caggiu Ilenia , Devinsky Orrin , Ferracuti Stefano , Focquaert Farah , Forgione Arianna , Gilbert Fredric , Pennati Ambrogio , Pietrini Pietro , Rainero Innocenzo , Sartori Giuseppe , Swerdlow Russell , Camperio Ciani Andrea S. 
Published: January 2023

AbstractIdiopathic and acquired pedophilia are two different disorders with two different etiologies. However, the differential diagnosis is still very difficult, as the behavioral indicators used to discriminate the two forms of pedophilia are underexplored, and clinicians are still devoid of clear guidelines describing the clinical and neuroscientific investigations suggested to help them with this difficult task. Furthermore, the consequences of misdiagnosis are not known, and a consensus regarding the legal consequences for the two kinds of offenders is still lacking. The present study used the Delphi method to reach a global consensus on the following six topics: behavioral indicators/red flags helpful for differential diagnosis; neurological conditions potentially leading to acquired pedophilia; neuroscientific investigations important for a correct understanding of the case; consequences of misdiagnosis; legal consequences; and issues and future perspectives. An international and multidisciplinary board of scientists and clinicians took part in the consensus statements as Delphi members. The Delphi panel comprised 52 raters with interdisciplinary competencies, including neurologists, psychiatrists, neuropsychologists, forensic psychologists, expert in ethics, etc. The final recommendations consisted of 63 statements covering the six different topics. The current study is the first expert consensus on a delicate topic such as pedophilia. Important exploitable consensual recommendations that can ultimately be of immediate use by clinicians to help with differential diagnosis and plan and guide therapeutic interventions are described, as well as future perspectives for researchers.
Osteocyte Egln1/Phd2 links oxygen sensing and biomineralization via FGF23
Authors: Noonan Megan L. , Ni Pu , Solis Emmanuel , Marambio Yamil G. , Agoro Rafiou , Chu Xiaona , Wang Yue , Gao Hongyu , Xuei Xiaoling , Clinkenbeard Erica L. , Jiang Guanglong , Liu Sheng , Stegen Steve , Carmeliet Geert , Thompson William R. , Liu Yunlong , Wan Jun , White Kenneth E. 
Published: January 2023

AbstractOsteocytes act within a hypoxic environment to control key steps in bone formation. FGF23, a critical phosphate-regulating hormone, is stimulated by low oxygen/iron in acute and chronic diseases, however the molecular mechanisms directing this process remain unclear. Our goal was to identify the osteocyte factors responsible for FGF23 production driven by changes in oxygen/iron utilization. Hypoxia-inducible factor-prolyl hydroxylase inhibitors (HIF-PHI) which stabilize HIF transcription factors, increased Fgf23 in normal mice, as well as in osteocyte-like cells; in mice with conditional osteocyte Fgf23 deletion, circulating iFGF23 was suppressed. An inducible MSC cell line (‘MPC2’) underwent FG-4592 treatment and ATACseq/RNAseq, and demonstrated that differentiated osteocytes significantly increased HIF genomic accessibility versus progenitor cells. Integrative genomics also revealed increased prolyl hydroxylase Egln1 (Phd2) chromatin accessibility and expression, which was positively associated with osteocyte differentiation. In mice with chronic kidney disease (CKD), Phd1-3 enzymes were suppressed, consistent with FGF23 upregulation in this model. Conditional loss of Phd2 from osteocytes in vivo resulted in upregulated Fgf23, in line with our findings that the MPC2 cell line lacking Phd2 (CRISPR Phd2-KO cells) constitutively activated Fgf23 that was abolished by HIF1α blockade. In vitro, Phd2-KO cells lost iron-mediated suppression of Fgf23 and this activity was not compensated for by Phd1 or −3. In sum, osteocytes become adapted to oxygen/iron sensing during differentiation and are directly sensitive to bioavailable iron. Further, Phd2 is a critical mediator of osteocyte FGF23 production, thus our collective studies may provide new therapeutic targets for skeletal diseases involving disturbed oxygen/iron sensing.
Nuclear SPHK2/S1P induces oxidative stress and NLRP3 inflammasome activation via promoting p53 acetylation in lipopolysaccharide-induced acute lung injury
Authors: Gong Linjing , Shen Yue , Wang Sijiao , Wang Xinyuan , Ji Haiying , Wu Xu , Hu Lijuan , Zhu Lei 
Published: January 2023

AbstractA bulk of evidence identified that macrophages, including resident alveolar macrophages and recruited macrophages from the blood, played an important role in the pathogenesis of acute respiratory distress syndrome (ARDS). However, the molecular mechanisms of macrophages-induced acute lung injury (ALI) by facilitating oxidative stress and inflammatory responses remain unclear. Herein, we noticed that the levels of mitochondrial reactive oxygen species (mtROS), SPHK2 and activated NLRP3 inflammasome were higher in peripheral blood mononuclear cells (PBMCs) of ARDS patients than that in healthy volunteers. Similar observations were recapitulated in LPS-treated RAW264.7 and THP-1 cells. After exposure to LPS, the SPHK2 enzymatic activity, NLRP3 inflammasome activation and mtROS were significantly upregulated in macrophages. Moreover, knockdown SPHK2 via shRNA or inhibition SPHK2 could prominently decrease LPS-induced M1 macrophage polarization, oxidative stress and NLRP3 inflammasome activation. Further study indicated that upregulated SPHK2 could increase nuclear sphingosine-1-phosphate (S1P) levels and then restrict the enzyme activity of HDACs to facilitate p53 acetylation. Acetylation of p53 reinforced its binding to the specific region of the NLRP3 promoter and drove expression of NLRP3. In the in vivo experiments, it was also observed that treating with Opaganib (ABC294640), a specific SPHK2 inhibitor, could observably alleviate LPS-induced ALI, evidencing by lowered infiltration of inflammatory cells, increased M2 macrophages polarization and reduced oxidative damage in lung tissues. Besides, SPHK2 inhibition can also decrease the accumulation of acetylated p53 protein and the activation of NLRP3 inflammasome. Taken together, our results demonstrated for the first time that nuclear S1P can regulate the acetylation levels of non-histone protein through affecting HDACs enzyme activities, linking them to oxidative stress and inflammation in response to environmental signals. These data provide a theoretical basis that SPHK2 may be an effective therapeutic target of ARDS.
Diversity and distribution of mitochondrial DNA in non-Austronesian-speaking Taiwanese individuals
Authors: Lin Marie , Trejaut Jean A. 
Published: January 2023

AbstractMany studies have described the diversity of Austronesian-speaking Taiwanese people to shed more light on their origin and their connection with the “Out of Taiwan” migrations. However, the genetic relationship between the non-Austronesian-speaking groups of Taiwan and the populations of continental Asia is still unclear. Here, we studied the diversity of mtDNA in 767 non-Austronesian speakers from 16 locations in Taiwan using partial sequencing obtained from the hypervariable segment I (HVS-I) and coding regions 8,001-9,000 and 9.801–10,900 and 85 complete mtDNA genome sequences. Bayesian analysis of population structure was used to examine their relationship with over 3662 individuals representing indigenous groups of Taiwan, continental East Asia, Japan, and Island Southeast Asia. The whole analysis identified 278 haplotypes. Complete genomes revealed 62 novel subhaplogroups, of which 31 were exclusive to Taiwan. Estimates of coalescence times of all subhaplogroups showed peaks of diversification greater than 5.0 kya, likely characterizing gene flow from continental East Asian groups but not excluding in situ Taiwanese ancestry. Furthermore, a significant number of clades exclusive to non-Austronesian speakers of Taiwan (NAN_Tw) showed coalescence peaks between 1.0 and 2.6 kya, suggesting possible late Neolithic to early metal age settlements of NAN_Tw and local expansion in Taiwan.
Position-dependent effects of RNA-binding proteins in the context of co-transcriptional splicing
Authors: Horn Timur , Gosliga Alison , Li Congxin , Enculescu Mihaela , Legewie Stefan 
Published: January 2023

AbstractAlternative splicing is an important step in eukaryotic mRNA pre-processing which increases the complexity of gene expression programs, but is frequently altered in disease. Previous work on the regulation of alternative splicing has demonstrated that splicing is controlled by RNA-binding proteins (RBPs) and by epigenetic DNA/histone modifications which affect splicing by changing the speed of polymerase-mediated pre-mRNA transcription. The interplay of these different layers of splicing regulation is poorly understood. In this paper, we derived mathematical models describing how splicing decisions in a three-exon gene are made by combinatorial spliceosome binding to splice sites during ongoing transcription. We additionally take into account the effect of a regulatory RBP and find that the RBP binding position within the sequence is a key determinant of how RNA polymerase velocity affects splicing. Based on these results, we explain paradoxical observations in the experimental literature and further derive rules explaining why the same RBP can act as inhibitor or activator of cassette exon inclusion depending on its binding position. Finally, we derive a stochastic description of co-transcriptional splicing regulation at the single-cell level and show that splicing outcomes show little noise and follow a binomial distribution despite complex regulation by a multitude of factors. Taken together, our simulations demonstrate the robustness of splicing outcomes and reveal that quantitative insights into kinetic competition of co-transcriptional events are required to fully understand this important mechanism of gene expression diversity.