bluuebunny/crossref_metadata_2025_split huggingface dataset:
Effects of naringin on oxidative stress, inflammation, some reproductive parameters, and apoptosis in acrylamide‐induced testis toxicity in rat
Authors: Sengul Emin , Gelen Volkan , Yildirim Serkan , Cinar İrfan , Aksu Emrah Hicazi 
Published: January 2023

AbstractAcrylamide (ACR) is used in many fields such as cosmetics, paper, and textile industries. It also occurs at very high temperatures in some foods. Gonadotoxic effects of ACR have been found in experimental animals. Many studies use flavonoids to prevent the reproductive side effects of ACR. Naringin (NA) is a flavonoid and it has been determined by studies that it has no toxic effect on tissues. In our study, we aimed to determine the protective effect of NA against the damage of ACR on testicular tissue and the reproductive system in rats. In our study, 50 Spraque Dawley male rats weighing 220‐250 grams were used. Control: Only intragastric saline was administered for 10 days. ACR: Animals received ACR (40 mg/kg, intraperitoneally) for 10 days. NA50+ACR: Animals were given NA for 10 days and each NA was one hour after the administration of ACR. NA100+ACR: Animals received NA for 10 days and one hour after each NA was given ACR. NA100: Animals were given NA for 10 days. At the end of the applications, the rats were euthanized by cervical dislocation under anesthesia. Serum FSH, LH, and Dihydrotestosterone levels were compared between the groups. In addition, oxidative stress, inflammation, expression of some reproductive enzymes, and apoptosis markers were determined in testicular tissues. When these parameters were compared between groups, ACR induced testicular dysfunction and tissue damage in rats. We determined that only the NA application did not cause tissue damage. and the administration of NA along with ACR significantly reduced ACR‐induced testis toxicity.
Review on micro nano bubble technology for condensation heat transfer
Authors: Senthilkumar Gnanamani , Balaje Sekar Mangaiyarkkarasi , Jayaseelan Ellappan 
Published: January 2023

AbstractThe objective of this review paper is to implement and investigate of micro‐nano bubble technology for enhancement of condensation heat transfer. Micro‐nano bubbles dispersions in aqueous solutions can stay for a substantial time. As an example, 20 micron size micro‐nano bubbles take daily to rise one meter. Because the inertia force of fluid current exceeds the buoyant force, any currents within a large vessel would be anticipated to entrain kind of micro‐nano bubble dispersion. Micro‐nano bubbles will vaporize hotter water toward the center of the plume and convert that extra heat of transition to the cold wall, where it will condense the vapors and release latent heat if the condenser is arranged in the micro‐nano bubble‐assisted airlift configuration.
Update on canine anorchia: A review
Authors: Cicirelli Vincenzo , Burgio Matteo , Mrenoshki Daniela , Cseh Sandor , Aiudi Giulio , Lacalandra Giovanni Michele 
Published: January 2023

AbstractAbnormalities of the external genitals are an important issue in dog breeding because of the unfavourable qualities and characteristics of breeds, resulting in consistent economic losses. Despite their significance, little scientific attention has been given to these problems. Although there are several reviews on cryptorchidism in dogs, none have described anorchia. Testicular agenesis is a rare reproductive disorder with a congenital origin. Moreover, no author has described the diagnostic procedure for making a definitive diagnosis of anorchia in dogs. It is important to have a well‐structured diagnostic scheme to help practical veterinarians make a confirmatory diagnosis. This review article aims to provide an update on canine anorchia diagnosis based on the poor research studies published in recent years. We have also contributed to the pathogenesis of this disorder using human medicine studies. Finally, the review includes therapeutic hypotheses that can be expanded in future studies.
Iranian Children’s Drawings of God: Demographic and Contextual Considerations
Authors: Khodayarifard Mohammad , Pourhosein Reza , Pakdaman Shahla , Zandi Saeid 
Published: January 2023

AbstractIn 2014, we collected more than 3000 drawings of God in Iran. Here we present the conditions for this collection and the results derived from it. We interpret our findings from the perspective of developmental psychology, and discuss them in terms of social, cultural, and contextual factors (media, formal and informal education). We consider God representation with regard to Iranian-Islamic culture. Additionally, we make a brief comparison between our findings, drawn from participants in Iran, and the findings of studies conducted in Western cultures. Finally, limitations of the study and future research directions are critically discussed.
Clustered PHD domains in KMT2/MLL proteins are attracted by H3K4me3 and H3 acetylation-rich active promoters and enhancers
Authors: Stroynowska-Czerwinska Anna Maria , Klimczak Magdalena , Pastor Michal , Kazrani Asgar Abbas , Misztal Katarzyna , Bochtler Matthias 
Published: January 2023

AbstractHistone lysine-specific methyltransferase 2 (KMT2A-D) proteins, alternatively called mixed lineage leukemia (MLL1-4) proteins, mediate positive transcriptional memory. Acting as the catalytic subunits of human COMPASS-like complexes, KMT2A-D methylate H3K4 at promoters and enhancers. KMT2A-D contain understudied highly conserved triplets and a quartet of plant homeodomains (PHDs). Here, we show that all clustered (multiple) PHDs localize to the well-defined loci of H3K4me3 and H3 acetylation-rich active promoters and enhancers. Surprisingly, we observe little difference in binding pattern between PHDs from promoter-specific KMT2A-B and enhancer-specific KMT2C-D. Fusion of the KMT2A CXXC domain to the PHDs drastically enhances their preference for promoters over enhancers. Hence, the presence of CXXC domains in KMT2A-B, but not KMT2C-D, may explain the promoter/enhancer preferences of the full-length proteins. Importantly, targets of PHDs overlap with KMT2A targets and are enriched in genes involved in the cancer pathways. We also observe that PHDs of KMT2A-D are mutated in cancer, especially within conserved folding motifs (Cys4HisCys2Cys/His). The mutations cause a domain loss-of-function. Taken together, our data suggest that PHDs of KMT2A-D guide the full-length proteins to active promoters and enhancers, and thus play a role in positive transcriptional memory.
Graphical Abstract
The new German evidence-based guideline on diffuse large B-cell lymphoma—key aspects for radiation oncologists
Authors: Oertel Michael , Berdel Christian , Held Gerhard , Herfarth Klaus , Schmidberger Heinz , Ernst Moritz , Lenz Georg , Borchmann Peter , Eich Hans Theodor 
Published: January 2023

Abstract
Purpose
Diffuse large B‑cell lymphoma (DLBCL) is an aggressive lymphoma subtype treated successfully with immunochemotherapy. However, there are conflicting data on the role and impact of consolidative radiation therapy (RT). The publication of the national evidence-based guideline on DLBCL prompted us to review relevant passages on radiation oncology.

Methods
The following article reviews the evidence and recommendations given in the current German evidence-based guideline on DLBCL regarding RT and summarizes pivotal aspects. Additional literature is presented to provide a comprehensive background for the published recommendations.

Results
RT shall be administered to all patients with localized positron emission tomography(PET)-positive residues after completion of immunochemotherapy and should use a dose of 30–40 Gray in normofractionation. For RT planning, PET information before and after immunochemotherapy shall be used, with either a PET-CT in the RT treatment position or an image fusion to the planning CT. Conformal techniques shall be used for target volume coverage, with a risk–benefit evaluation for the individual patient. Additionally, RT may be used in the treatment context of various subtypes of DLBCL as well as in the recurrent or refractory treatment situation.

Conclusion
RT remains an integral part of the treatment repertoire of DLBCL. With the use of PET-guided treatment, RT is indicated for patients with metabolically active tumors. In the context of the ongoing development of targeted therapies, new RT indications may evolve.
Particulate cartilage and platelet-rich plasma treatment for knee chondral defects in sheep
Authors: Alcaide-Ruggiero Lourdes , Molina-Hernández Verónica , Morgaz Juan , Fernández-Sarmiento J. Andrés , Granados María M. , Navarrete-Calvo Rocío , Pérez José , Quirós-Carmona Setefilla , Carrillo José M. , Cugat Ramón , Domínguez Juan M. 
Published: January 2023

Abstract
Purpose
Articular cartilage is vulnerable to multiple types of damage and it has limited reparative and regenerative capacities due to its absence of vascularity. Although a large number of therapeutic strategies exist to treat chondral defects, they have some limitations, such as fibrocartilage formation. Therefore, the goal of the present study was to evaluate the chondrogenic regenerative properties of an autologous-made matrix of particulated cartilage and platelet-rich plasma (PACI + PRP) implantation for the treatment of full-thickness chondral defects in sheep.

Methods
A full-thickness 8 mm diameter cartilage defect was created in the weight-bearing area of the medial femoral condyle in both knees of 16 sheep. The right knees of all animals were treated with particulated autograft cartilage implantation and platelet-rich plasma, while the left knees were injected with Ringer’s lactate solution or hyaluronic acid. The sheep were killed 9 or 18 months after surgery. Macroscopic evaluations were performed using three different scoring systems, and histopathological evaluations were performed using a modified scoring system based on different scoring systems.

Results
The PACI + PRP groups showed statistically significant differences in the percentage of defect repair and chondrocytes in the newly formed cartilage tissue at 18 months compared to 9 months.

Conclusions
The results suggest that macroscopic appearance, histological structure and chondrocyte repair were improved when using PACI + PRP treatment for chondral defects, producing an outcome similar to the surrounding healthy cartilage. PACI + PRP is a totally autologous, easy, and unexpensive treatment that can be performed in one-step procedure and is useful as a therapeutic option for knee chondral defects.
Effect of four-week cannabidiol treatment on cognitive function: secondary outcomes from a randomised clinical trial for the treatment of cannabis use disorder
Authors: Lees Rachel , Hines Lindsey A. , Hindocha Chandni , Baio Gianluca , Shaban Natacha D. C. , Stothart George , Mofeez Ali , Morgan Celia J. A. , Curran H. Valerie , Freeman Tom P. 
Published: January 2023

Abstract

Rationale
Chronic cannabis use is associated with impaired cognitive function. Evidence indicates cannabidiol (CBD) might be beneficial for treating cannabis use disorder. CBD may also have pro-cognitive effects; however, its effect on cognition in people with cannabis use disorder is currently unclear.

Objectives
We aimed to assess whether a 4-week CBD treatment impacted cognitive function. We hypothesised that CBD treatment would improve cognition from baseline to week 4, compared to placebo.

Methods
Cognition was assessed as a secondary outcome in a phase 2a randomised, double-blind, parallel-group and placebo-controlled clinical trial of 4-week daily 200 mg, 400 mg and 800 mg CBD for the treatment of cannabis use disorder. Participants had moderate or severe DSM-5 cannabis use disorder and intended to quit cannabis use. Our pre-registered primary cognitive outcome was delayed prose recall. Secondary cognitive outcomes were immediate prose recall, stop signal reaction time, trail-making task performance, verbal fluency and digit span.

Results
Seventy participants were randomly assigned to placebo (n = 23), 400 mg CBD (n = 24) and 800 mg CBD (n = 23). A 200 mg group was eliminated from the trial because it was an inefficacious dose at interim analysis (n = 12) and was not analysed here. For the primary cognitive outcome, there was no effect of CBD compared to placebo, evidenced by a lack of dose-by-time interaction at 400 mg (0.46, 95%CIs: − 1.41, 2.54) and 800 mg (0.89, 95%CIs: − 0.99, 2.81). There was no effect of CBD compared to placebo on secondary cognitive outcomes, except backwards digit span which increased following 800 mg CBD (0.30, 95%CIs: 0.02, 0.58).

Conclusions
In this clinical trial for cannabis use disorder, CBD did not influence delayed verbal memory. CBD did not have broad cognitive effects but 800 mg daily treatment may improve working memory manipulation.

Clinical trial registration
The trial was registered with ClinicalTrials.gov (NCT02044809) and the EU Clinical Trials Register (2013–000,361-36).
The microbiome of kidney stones and urine of patients with nephrolithiasis
Authors: Lemberger Ursula , Pjevac Petra , Hausmann Bela , Berry David , Moser Daniel , Jahrreis Victoria , Özsoy Mehmet , Shariat Shahrokh F. , Veser Julian 
Published: January 2023

AbstractThe incidence of nephrolithiasis is rising worldwide. Although it is a multifactorial disease, lifestyle plays a major role in its etiology. Another considerable factor could be an aberrant microbiome. In our observational single-center study, we aimed to investigate the composition of bacteria in kidney stones and urine focusing on patients with features of metabolic syndrome. Catheterized urine and kidney stones were collected prospectively from 100 consecutive patients undergoing endoscopic nephrolithotomy between 2020 and 2021 at our clinic. Microbiome composition was analyzed via 16S rRNA gene amplicon sequencing. Detection of bacteria was successful in 24% of the analyzed kidney stones. These patients had a prolonged length of stay compared to patients without verifiable bacteria in their stones (2.9 vs 1.5 days). Patients with features of metabolic syndrome were characterized by kidney stones colonized with classical gastrointestinal bacteria and displayed a significant enrichment of Enterococcaceae and Enterobacteriaceae. Stones of patients without features of metabolic syndrome characterized by Ureaplasma and Staphylococcaceae. Patients with bacteria in their kidney stones exhibit a longer length of stay, possibly due to more complex care. Patients presenting with features of metabolic syndrome displayed a distinct stone microbiome compared to metabolically fit patients. Understanding the role of bacteria in stone formation could enable targeted therapy, prevention of post-operative complications and new therapeutic strategies.
Benefits of PECS Block as Part of the Enhanced Recovery After Surgery (ERAS) Protocol for Breast Cancer Surgery in an Asian Institution: A Retrospective Cohort Study
Authors: Diana Kavinya , Teh Mei‐Sze , Islam Tania , Lim Woon‐Lai , Beh Zhi‐Yuan , Taib Nur Aishah Mohd 
Published: January 2023

AbstractIntroductionRegional analgesia techniques have been increasingly used for post‐operative pain management following mastectomy. We aim to evaluate analgesic benefits of pectoral nerve (PECS2) block incorporated as part of the enhanced recovery after surgery (ERAS) protocol in patients undergoing mastectomy in University Malaya Medical Centre, Malaysia.Material and methodsA single centre, cohort study evaluating 335 women who have undergone unilateral mastectomy between January 2017 and March 2020 in Malaysia. Regional anaesthesia were given pre‐operatively via ultrasound guided pectoral and intercostal nerves block (PECSII).ResultsUtilization of regional anaesthesia increased from 11% in 2017 to 43% in 2020. Types and duration of surgeries were comparable. Opiod consumption was 3 mg lower in those who had PECS2 block ((27 [24–30] mg), in comparison with those who received general anaesthesia only (30 [26–34] mg), p < 0.001, and length of stay was half a day shorter in the regional anaesthesia group and these were statistically significant. However, pain score (2 [1–3]; 2 [1–3], p=0.719) and post‐operative nausea and vomiting (PONV) (32.6–32.5%, p = 0.996) were similar.ConclusionThis study highlights the importance of PECS2 block as a component of ERAS protocol for mastectomy in an Asian hospital. This study also inferred that patients may be safely discharged within 24 h of surgery and therefore, same day surgery may be feasible in selected group of patients undergoing mastectomy and this could imply overall cost benefits.
Chloroacetate esterase reaction combined with immunohistochemical characterization of the specific tissue microenvironment of mast cells
Authors: Atiakshin Dmitri , Kostin Andrey , Buchwalow Igor , Morozow Denis , Samoilova Vera , Tiemann Markus 
Published: January 2023

AbstractThis study provides a combined histochemical method for detecting enzyme activity of chloroacetate esterase simultaneously with immunolabeling of the components of a specific tissue microenvironment on formalin-fixed, paraffin-embedded specimens. Chromogenic detection of the molecular targets within and outside the mast cells provides novel options in determining the histoarchitectonics of organ-specific mast cell populations, studying the functional significance of chloroacetate esterase and specifying the immune landscape of the tissue microenvironment.
Recovery and stress of control center dispatchers in the first waves of the SARS-CoV-2 pandemic
Authors: Schumann Heiko , Thielmann Beatrice , Botscharow Julia , Böckelmann Irina 
Published: January 2023

SummaryControl center dispatchers (CCDs) are exposed to high levels of work-related mental stress, which are exacerbated by the current severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic. The aim of this study was to comparatively analyze the recovery and stress state of CCDs during the first and second waves of the SARS-CoV‑2 pandemic. A total of 490 CCDs (n = 440, t1 and n = 50, t2) with a mean age of 42.26 ± 8.79 years participated directly at the end of the first wave from June to August 2020 (t1) and during the second wave between January and February 2021 (t2) of the SARS-CoV‑2 pandemic. The short form (EBF-24/A; test form S2) of the Kallus recovery-stress questionnaire (EBF) was applied. Over the course of the two survey phases, the dimensions strain significantly increased (t1: 2.47 ± 1.08 vs. t2: 3.12 ± 0.93 points, p < 0.001) and recovery significantly decreased (t1: 3.03 ± 0.94 vs. t2: 2.50 ± 0.81 points, p < 0.001). Significant to highly significant differences were present in the EBF dimensions of the two waves in the majority of cases, even when taking the waves into account. For the variable “recovery in the social field”, a medium effect was noticeable in the corrected model (η2 = 0.064). The SARS-CoV‑2 pandemic illustrates that the stress experience increased from the first to the second waves, and the recovery of CCDs decreased. These data provide a directional trend as the pandemic is ongoing, and stress and strain situations in control centers may continue to worsen. Immediate health promotion and prevention measures are essential.
Spinal cord stimulation in non-reconstructable critical limb ischemia: a retrospective study of 71 cases
Authors: Piedade G. S. , Vesper J. , Reichstein D. , Dauphin A. K. , Damirchi S. 
Published: January 2023

Abstract

Background

Spinal cord stimulation (SCS) is a therapeutic option for patients with a peripheral arterial disease with critical limb ischemia (CLI) and consequent ischemic rest pain. Neuromodulation is chosen when vascular reconstruction is not possible or failed. Data about the effect of SCS over limb salvage rates are dissonant.

Method
We report on a retrospective cohort of CLI patients who were implanted with SCS systems between July 2010 and December 2013 in a single center. Major amputation, postoperative complications, and death were recorded.

Results
Seventy-two CLI patients underwent SCS implantation, with 35 of them classified as non-reconstructable and 37 with previous but failed or only partially successful vascular procedures. A total of 21 subjects were at Fontaine’s stage III (29.2%), and the remaining 51 were at stage IV (70.8%). In total, 26.4% of the patients had diabetes (n = 19), two of them at Fontaine’s stage III. The mean follow-up was 17.1 ± 10.5 months. At the last follow-up, 59.2% of all patients (42/71), 85.7% of Fontaine’s stage III (18/21), 48.0% of Fontaine’s stage IV (24/50), and 52.6% of diabetic patients (10/19) were alive without major amputation. The probability of limb survival at 12 months was 72% for all patients, 94% for Fontaine’s stage III, 62% for Fontaine’s stage IV, and 61% for diabetic patients. The probability of survival at 12 months for patients who underwent major limb amputation (n = 25) was 86% with a mean survival time of 31.03 ± 4.63 months.

Conclusions
Non-reconstructable CLI patients treated with SCS can achieve meaningful clinical outcomes with few procedure-related complications. The therapy may be more beneficial in patients classified as Fontaine’s Stage III.
Mixed reality and sensor real-time feedback to increase muscle engagement during deep core exercising
Authors: Lancere L. , Jürgen M. , Gapeyeva H. 
Published: January 2023

AbstractIn lower extremity amputee rehabilitation programs, difficult-to-master targeted activation of deep core muscles and pursed-lip breathing training are prescribed to treat poor movement quality and to improve recovery after amputation. Non-invasive wireless sensors and mixed reality (MR) technologies are proposed as a solution. The main aim was to validate a novel rehabilitation technology by exploring whether a combined verbal and visual mixed reality feedback (VF + MR) will initiate a greater change in muscle electrical activation magnitude compared to verbal feedback only (VF) during exercising. The second objective was to evaluate the effectiveness of specific exercise program targeted to engage specifically deep core muscles. Pre-post-test cross-over study involved electromyographic activity (EMG) analysis from Transversus Abdominis (TA) and Multifidus (MF) muscles and self-reported questionnaires to evaluate the efficiency of MR feedback. Anthropometric data, state of health, subjective low back pain (Oswestry Disability Index), and physical activity level (IPAQ) estimation were analysed. The data from 13 patients following unilateral transtibial and transfemoral amputation showed a significant EMG increase in (VF + MR) for Chair Lean (p = 0.03) and Bent Leg Raise (p = 0.0005) exercises for TA muscle. Even though there was no significant difference in Back Bridge and Side Plank exercises, 6 to 10 participants depending on the exercise, had an increase of EMG in the range of 50–400% for both – TA and MF muscles. The proposed solution has a high potential for increasing motivation, self-awareness, and muscle engagement during exercises, based on EMG and self-reported questionnaire data.