bluuebunny/crossref_metadata_2025_split huggingface dataset:
The growth, lipid accumulation and adaptation mechanism in response to variation of temperature and nitrogen supply in psychrotrophic filamentous microalga Xanthonema hormidioides (Xanthophyceae)
Authors: Gao Baoyan , Hong Jian , Chen Jiamin , Zhang Hu , Hu Ren , Zhang Chengwu 
Published: January 2023

Abstract
Background
Microalgae are promising feedstocks for production of renewable biofuels and value-added bioproducts. Temperature and nitrogen supply are important environmental and nutritional factors affecting the growth and metabolism of microalgae, respectively. In this study, the growth and lipid accumulation of filamentous microalgae Xanthonema hormidioides under different temperatures (5, 7, 10, 15, 20, 25, 27 and 30 °C) and initial nitrogen concentrations (3, 9, 18 mM) were investigated, and its adaptive mechanisms of tolerance to low temperature and nitrogen stress were analysis by proteomics.

Results
The optimum temperature range for the growth of X. hormidioides was between 15 and 20 °C, and the algal cells had slow growth rate at 5 °C and could not survive at 30 °C. The maximum biomass concentration was 11.73 g L−1 under the temperature of 20 °C, and the highest total lipid content was 56.63% of dry weight. Low temperature did not change the fatty acids profiles but promoted the accumulation of unsaturated fatty acids of X. hormidioides. The maximum contents of palmitoleic acid, eicosapentaenoic acid and total fatty acid were 23.64%, 2.49% and 41.14% of dry weight, respectively. Proteomics was performed under three temperature (7, 15, 25 °C), two nitrogen concentrations (3 and 18 mM) and two cultivation times (day 3 and 12). A total of 6503 proteins were identified. In the low temperature, photosynthesis-related proteins were down-regulated to protect the photosynthetic apparatus. The up-regulation of key enzymes DGAT and PDAT demonstrated the accumulation of TAGs under low nitrogen treatment. The proteins related to ribosome, phosphatidylinositol signaling system, antioxidant system and cold shock proteins (CSPs) in X. hormidioides were co-upregulated under the treatment of low temperature, which can alleviate the damages induced by temperature stress and maintain the normal growth and metabolism of algal cells.

Conclusions
X. hormidioides is a psychrotolerant microalga. It is an oleaginous filamentous microalga containing hyper palmitoleic acid and a certain amount of eicosapentaenoic acid with great potential for biofuel development, as well as for applications in nutritional health products and other industries.
How can gender be identified from heart rate data? Evaluation using ALLSTAR heart rate variability big data analysis
Authors: Kaneko Itaru , Hayano Junichiro , Yuda Emi 
Published: January 2023

Abstract
Objective
A small electrocardiograph and Holter electrocardiograph can record an electrocardiogram for 24 h or more. We examined whether gender could be verified from such an electrocardiogram and, if possible, how accurate it would be.

Results
Ten dimensional statistics were extracted from the heart rate data of more than 420,000 people, and gender identification was performed by various major identification methods. Lasso, linear regression, SVM, random forest, logistic regression, k-means, Elastic Net were compared, for Age < 50 and Age ≥ 50. The best Accuracy was 0.681927 for Random Forest for Age < 50. There are no consistent difference between Age < 50 and Age ≥ 50. Although the discrimination results based on these statistics are statistically significant, it was confirmed that they are not accurate enough to determine the gender of an individual.
Improving the thermostability of GH49 dextranase AoDex by site-directed mutagenesis
Authors: Wei Zhen , Chen Jinling , Xu Linxiang , Liu Nannan , Yang Jie , Wang Shujun 
Published: January 2023

AbstractAs an indispensable enzyme for the hydrolysis of dextran, dextranase has been widely used in the fields of food and medicine. It should be noted that the weak thermostability of dextranase has become a restricted factor for industrial applications. This study aims to improve the thermostability of dextranase AoDex in glycoside hydrolase (GH) family 49 that derived from Arthrobacter oxydans KQ11. Some mutants were predicted and constructed based on B-factor analysis, PoPMuSiC and HotMuSiC algorithms, and four mutants exhibited higher heat resistance. Compared with the wild-type, mutant S357P showed the best improved thermostability with a 5.4-fold increase of half-life at 60 °C, and a 2.1-fold increase of half-life at 65 °C. Furthermore, S357V displayed the most obvious increase in enzymatic activity and thermostability simultaneously. Structural modeling analysis indicated that the improved thermostability of mutants might be attributed to the introduction of proline and hydrophobic effects, which generated the rigid optimization of the structural conformation. These results illustrated that it was effective to improve the thermostability of dextranase AoDex by rational design and site-directed mutagenesis. The thermostable mutant of dextranase AoDex has potential application value, and it can also provide references for engineering other thermostable dextranases of the GH49 family.
Trainable windows for SincNet architecture
Authors: H C Prashanth , Rao Madhav , Eledath Dhanya , V Ramasubramanian 
Published: January 2023

AbstractSincNet architecture has shown significant benefits over traditional Convolutional Neural Networks (CNN), especially for speaker recognition applications. SincNet comprises parameterized Sinc functions as filters in the first layer followed by convolutional layers. Although SincNet is compact in nature and offers top-level understanding of the features extracted, the effect of window function used in SincNet is not thoroughly addressed yet. Hamming and Hann are popularly used as the default time-localized windows to reduce spectral leakage. Hence, a comprehensive investigation of 28 different windowing functions on SincNet architecture towards speaker recognition task using TIMIT dataset was performed in this work. Additionally, “trainable” window functions were configured with tunable parameters to characterize the performance. The paper benchmarks the effect of the time-localized windowing function in terms of the bandwidth, side-lobe suppression, and spectral leakage for the filter banks employed in the first layer of the SincNet architecture. Trainable Gaussian and Cosine-Sum functions exhibited relative improvement of 41.46% and 82.11% in the sentence level classification error rate over Hamming window when employed on SincNet architecture.
Identifying effect modifiers of CAR-T cell therapeutic efficacy: a systematic review and individual patient data meta-analysis protocol
Authors: Lalu Manoj M. , Kekre Natasha , Montroy Joshua , Ghiasi Maryam , Hay Kevin , McComb Scott , Weeratna Risini , Atkins Harold , Hutton Brian , Yahya Ayel , Masurekar Ashish , Sobh Mohamad , Fergusson Dean A. 
Published: January 2023

Abstract
Background
Chimeric antigen receptor T cell therapy (CAR-T) represents a promising and exciting new therapy for hematologic malignancies, where prognosis for relapsed/refractory patients remains poor. Encouraging results from clinical trials have often been tempered by heterogeneity in response to treatment among patients, as well as safety concerns including cytokine release syndrome. The identification of specific patient or treatment-specific factors underlying this heterogeneity may provide the key to the long-term sustainability of this complex and expensive therapy. An individual patient data meta-analysis (IPMDA) may provide potential explanations for the high degree of heterogeneity. Therefore, our objective is to perform a systematic review and IPDMA of CAR-T cell therapy in patients with hematologic malignancies to explore potential effect modifiers of CAR-T cell therapy.

Methods and analysis
We will search MEDLINE, Embase, and the Cochrane Central Register of Controlled Clinical Trials. Studies will be screened in duplicate at the abstract level, then at the full-text level by two independent reviewers. We will include any prospective clinical trial of CAR-T cell therapy in patients with hematologic malignancies. Our primary outcome is complete response, while secondary outcomes of interest include overall response, progression-free survival, overall survival, and safety. IPD will be collected from each included trial and, in the case of missing data, corresponding authors/study sponsors will be contacted. Standard aggregate meta-analyses will be performed, followed by the IPD meta-analysis using a one-stage approach. A modified Institute of Health Economics tool will be used to evaluate the risk of bias of included studies.

Ethics and dissemination
Identifying characteristics that may act as modifiers of CAR-T cell efficacy is of paramount importance and can help shape future clinical trials in the field. Results from this study will be submitted for publication in a peer-reviewed scientific journal, presented at relevant conferences and shared with relevant stakeholders.
Significant increase of serum extracellular vesicle-packaged growth differentiation factor 15 in type 2 diabetes mellitus: a cross-sectional study
Authors: Zhao Wen , Li Xinwei , Li Xinxin , Peng Lu , Li Yu , Du Yunhui , He Jianxun , Qin Yanwen , Zhang Huina 
Published: January 2023

Abstract
Background
Growth differentiation factor 15 (GDF15) is a stress-inducible factor involved in the inflammatory progression of many complications, including type 2 diabetes mellitus (T2DM). Growing evidence suggests that molecules in extracellular vesicles (EVs) are associated with diabetes or diabetes-related complications. However, the correlation between serum extracellular vesicle-derived growth differentiation factor15 (EV-GDF15) and T2DM is unknown. The aim of this cross-sectional study is to investigate whether serum EV-GDF15 is associated with T2DM incidence.

Methods
116 individuals, including 78 T2DM and 38 non-T2DM, were recruited as participants. The concentrations of serum EV-GDF15 and serum GDF15 were determined by Luminex assay. Serum EVs were obtained by ultracentrifugation. Multivariate stepwise regression analysis was used to determine the association between serum GDF15 levels and fasting plasma glucose (FPG) as well as glycated hemoglobin (HbA1c). The association of serum EV-GDF15 levels with T2DM was determined by multivariate logistic regression analysis.

Results
Our data showed that the levels of serum EV-GDF15 and serum GDF15 were significantly increased in T2DM patients compared with non-T2DM subjects (EV-GDF15 levels, 13.68 (6.61–23.44) pg/mL vs. 5.56 (3.44–12.09) pg/mL, P < 0.001; and serum GDF15 levels, 1025.49 (677.87–1626.36) pg/mL vs. 675.46 (469.53–919.98) pg/mL, P < 0.001). There was a linear correlation between EV-GDF15 levels and fasting plasma glucose (FPG) and Hemoglobin A1C (HbA1c) levels (normalized β = 0.357, P < 0.001; normalized β = 0.409, P < 0.001, respectively). Elevated levels of EV-GDF15 were accompanied by an increase in the proportion of patients with T2DM (from 47.5 to 78.9%) and a progressive independent association with the incidence of T2DM (from OR = 3.06, 95% CI 1.02–9.19, P = 0.047 to OR = 3.75, 95% CI 1.14–12.26, P = 0.029). Notably, high levels of serum GDF15 plus high levels of serum EV-GDF15 were significantly associated with T2DM more than either alone.

Conclusion
This study elucidated that increased levels of GDF15 in serum EVs were independently associated with T2DM.
Laboratory measurements show temperature-dependent permittivity of lunar regolith simulants
Authors: Kobayashi M. , Miyamoto H. , Pál B. D. , Niihara T. , Takemura T. 
Published: January 2023

AbstractThe mapping of available water–ice is a crucial step in the lunar exploration missions. Ground penetrating radars have the potential to map the subsurface structure and the existence of water–ice in terms of the electromagnetic properties, specifically, the permittivity. Slight differences in permittivity can be significantly important when applied in a dry environment, such as on the Moon and Mars. The capability of detecting a small fraction of putative water–ice depends on the permittivity changes in terms of its dependent parameters, such as the frequency, the temperature, the porosity, and the chemical composition. Our work aims at mitigating false detection or overlooking of water–ce by considering their conditions that previous researches did not cover. We measured the permittivity of different lunar regolith relevant analogue samples with a fixed 40 % porosity in the ultra-high-frequency–super-high-frequency band. We used the coaxial probe method to measure anorthosite, basalt, dunite and ilmenite at$$20\,^\circ \hbox {C}$$20∘C,$$-20\,^\circ \hbox {C}$$-20∘Cand$$-60\,^\circ \hbox {C}$$-60∘C, and we find that, at$$-60\,^\circ \hbox {C}$$-60∘C, the permittivity decreases about 6–18 % compared with the values at$$20\,^\circ \hbox {C}$$20∘C. Within this temperature range, the permittivity is quite similar to the permittivity of water–ice. We find that the conventional calculation would overestimate the permittivity in the low temperature areas, such as the permanently shadowed regions. We also find that each component in the lunar regolith has different temperature-dependent permittivity, which might be important for radar data analysis to detect lunar polar water–ice. Our results also suggest that it should be possible to estimate the water–ice content from radar measurements at different temperatures given an appropriate method.Graphical Abstract
Middle Miocene forearc alkaline magmatism in Amami-Oshima Island, central Ryukyu Arc: implications for paleoreconstruction of Shikoku Basin
Authors: Motohashi Ginta , Ishizuka Osamu , Oda Hirokuni , Sano Takashi , Sekimoto Shun , Ujiie Kohtaro 
Published: January 2023

AbstractDuring the middle Miocene, forearc alkaline magmatism occurred in the Outer Zone of Southwest Japan and the northern Ryukyu Arc, resulting in the formation of forearc alkaline basaltic rocks with ocean island basalt (OIB)-like chemical signatures. In contrast, subduction-related magmatism was present in the central Ryukyu Arc. However, the southwestern margin of the forearc alkaline magmatism was poorly constrained in the Ryukyu Arc. We examined two basaltic dikes in the Chichibu accretionary complex of Amami-Oshima Island, the central Ryukyu Arc. The dikes cut massive basalt, reddish chert, varicolored shale, and grey chert. The chemical compositions of basaltic dikes are characterized by the enrichment of incompatible trace elements, possibly representing a low degree of partial melting from a deep mantle source. 40Ar/39Ar dating analyses indicate that the basaltic dikes yield ages of 16.37 ± 0.14 Ma and 16.51 ± 0.10 Ma. The paleomagnetic analyses on stepwise thermal demagnetizations allowed extracting the direction of stable magnetizations with unblocking temperatures of 450–575 °C for the two dikes [(Dec, Inc) = (138.1°, − 13.3°), (124.0°, − 24.9°)]. The magnetization could be primary, acquired either as part of a secular variation or a geomagnetic excursion during reversed polarity chron/subchron. The 40Ar/39Ar ages and paleomagnetic directions within the error range imply that they intruded almost simultaneously during C5Cn.2r (16.532–16.434 Ma). The age and trace element patterns of the basaltic dikes are comparable to those of Miocene alkaline basaltic rocks, which resulted from forearc alkaline magmatism during the initial subduction of the young and warm Shikoku Basin. The discovery of alkaline basaltic dikes on Amami-Oshima Island suggests that the distribution of middle Miocene forearc alkaline magmatism may extend to the central Ryukyu Arc. Hence, the northern end of the Kyushu-Palau Ridge (i.e., southern end of Shikoku Basin) could have been located south of Amami-Oshima Island around 16.5–16.4 Ma, then moved eastward to the current location.
Graphical Abstract
Partial hepatectomy for a patient with Rendu–Osler–Weber disease: a case report
Authors: Sekiguchi Naoko , Yamada Daisaku , Kobayashi Shogo , Sasaki Kazuki , Iwagami Yoshifumi , Tomimaru Yoshito , Noda Takehiro , Takahashi Hidenori , Doki Yuichiro , Eguchi Hidetoshi 
Published: January 2023

Abstract
Background
Rendu–Osler–Weber disease (Osler disease) is a genetic disease with an autosomal dominant inheritance pattern. It is characterized by widespread telangiectasia in multiple organs. Liver involvement of FNH is relatively common, but liver cancer is very rare, and there are few reports on hepatectomy or postoperative complications. We report a very rare case in which hepatectomy was performed for a patient with Osler disease.

Case presentation
The patient was a 39-year-old man with Osler disease who had been previously diagnosed with multiple FNH and who had been followed for 8 years. During follow-up, the diameter of an S6 lesion gradually increased from 30 to 50 mm; no other lesions increased in size. We decided to perform partial liver resection as total biopsy for the growing tumor, due to the possibility that the growing tumor lesion included malignant components. The pathological examination revealed no obvious malignancy, which was finally diagnosed FNH. The postoperative course was uneventful and he was discharged on the 14th day after surgery. In the second month after discharge, he was transferred to our hospital with sudden abdominal pain in the right hypochondrium with severe tenderness. CT showed extravasation of contrast medium from the hepatic dissection surface in S6, and the hematoma extended to the pelvic floor. Emergency IVR was performed and revealed leakage of the contrast medium from the A6 branch. We embolized the A6 with Lipiodol. After embolization, there were no major problems, and the patient was discharged on the 9th day after the treatment.

Conclusions
Postoperative hemorrhage often occurs within 24 h after surgery, and 2 months after surgery is considered to be the late stage of the wound healing process, and postoperative hemorrhage at this timepoint is considered rare. This unexpected delayed postoperative hemorrhage may have been related to the etiology and pathology of Osler disease, nevertheless, case reports of hepatectomy for patients with Osler disease are limited. We, therefore, report the present case with a review of the relevant literature.
Currencies of greater interest for central Asian economies: an analysis of exchange market pressure amid global and regional interdependence
Authors: Jain Devendra Kumar , Ur-Rehman Naqeeb , Ganiev Omonjon , Arora Kapil 
Published: January 2023

AbstractCentral Asian Economies (CAEs) have diverse exchange rate policies. They have recorded higher volatility in the foreign exchange market since inception. High volatility of the transition era has drifted these economies towards partial dollarization. Monetary authorities in CAEs, (already have a challenge of maintaining monetary policy autonomy) have a gigantic task of price stability and stopping the spread of dollarization. This study is directed towards assessing the drivers and the determinants of foreign exchange market pressure in CAEs. The results, based on panel data analysis and the System GMM model, have provided useful insights about the exchange market pressure determinants particularly USD, Euro, Ruble, and Renminbi. The results show that China and Russia exchange market pressure has a negative effect on the exchange market pressure of CAEs. While the dollar index shows a positive impact on the exchange market pressure of CAEs. Overall, the findings imply that China and Russia currency appreciation results in a trade deficit across CAEs. The policy implication suggests that the floating exchange rate regime (inflation targeting regime) is not in favor of CAEs, and they must use managed-float to reduce their trade deficits.
Empirical evidence on the ownership and liquidity of real estate tokens
Authors: Swinkels Laurens 
Published: January 2023

AbstractTo better understand the potential and limitations of the tokenization of real asset markets, empirical studies need to examine this radically new organization of financial markets. In our study, we examine the financial and economic consequences of tokenizing 58 residential rental properties in the US, particularly those in Detroit. Tokenization aims at fragmented ownership. We found that the residential properties examined have 254 owners on average. Investors with a greater than USD 5,000 investment in real estate tokens, diversify their real estate ownership across properties within and across the cities. Property ownership changes about once yearly, with more changes for properties on decentralized exchanges. We report that real estate token prices move according to the house price index; hence, investing in real estate tokens provides economic exposure to residential house prices.
A modified EDAS model for comparison of mobile wallet service providers in India
Authors: Biswas Sanjib , Pamucar Dragan 
Published: January 2023

AbstractThe present paper has two-fold purposes. First, the current work provides an integrated theoretical framework to compare popular mobile wallet service providers based on users' views in the Indian context. To this end, we propose a new grey correlation-based Picture Fuzzy-Evaluation based on Distance from Average Solution (GCPF-EDAS) framework for the comparative analysis. We integrate the fundamental framework of the Technology Acceptance Model and Unified theory of acceptance and use of technology vis-à-vis service quality dimensions for criteria selection. For comparative ranking, we conduct our analysis under uncertain environments using picture fuzzy numbers. We find that user-friendliness, a wide variety of use, and familiarity and awareness about the products help reduce the uncertainty factors and obtain positive impressions from the users. It is seen that PhonePe (A3), Google Pay (A2), Amazon Pay (A4) and PayTM (A1) hold top positions. For validation of the result, we first compare the ranking provided by our proposed model with that derived by using picture fuzzy score based extensions of EDAS and another widely used algorithm such as The Technique for Order of Preference by Similarity to Ideal Solution. We observe a significant consistency. We then carry out rank reversal test for GCPF-EDAS model. We notice that our proposed GCPF-EDAS model does not suffers from rank reversal phenomenon. To examine the stability in the result for further validation, we carry out the sensitivity analysis by varying the differentiating coefficient and exchanging the criteria weights. We find that our proposed method provides stable result for the present case study and performs better as ranking order does not get changed significantly with the changes in the given conditions.
Coping strategies for happy childless ageing: an explorative study in Poland
Authors: Abramowska-Kmon Anita , Mynarska Monika , Timoszuk Sylwia 
Published: January 2023

AbstractChildless older people face many challenges resulting from their lack of children that may have an impact on their subjective well-being, especially in a country where family ties are strong, and children are the main source of support. In this paper, we examine the coping strategies that childless seniors in Poland employ in order to mitigate various risks related to having no offspring. We use a qualitative approach, drawing upon in-depth interviews with 42 childless men and women aged 65 or older. We frame our analyses within the life course approach, stressing the biographical development of coping strategies over the life course. Our results corroborate previous findings, and show that the major challenges older childless people in Poland face are related to feeling lonely, and to having insufficient (practical) support and care in case of dependency. We describe the actions the respondents consciously took in response to these challenges, and examine how these actions changed over the life course. The strategies aimed at promoting healthy ageing or helping older people expand their social networks are strongly in line with the respondents’ personal predispositions and earlier lifestyles and experiences.