bluuebunny/crossref_metadata_2025_split huggingface dataset:
Genetic Investigation of the Trail Mechanism in Diabetic and Non- Diabetic Obese Patients
Authors: SEZGIN Saadet Busra AKSOYER , DURAK Sermin , CELIK Faruk , Gheybi Arezoo , Diramali Murat , Cakmak Ramazan , Gurol Ali Osman , Yaylim Ilhan , Zeybek Umit 
Published: June 2023

Abstract
Background
Obesity is an important healthcare issue caused by abnormally increased adipose tissue because of energy-intake overcoming energy expenditure. Disturbances in the physiological function of adipose tissue mediate the development of diabetes. It is a metabolic disease that results from decreased insulin-levels and/or changes in the insulin action mechanism. Tumor Necrosis Factor-Associated Apoptosis-Inducing Ligand(TRAIL), which is a member of the Tumor Necrosis Factor(TNF)-family with an important role in adipose tissue biology, is included in many studies with its ability to induce apoptosis in cancer cells, but the number of human-studies conducted on the gene related to its protective-role against diabetes and obesity at this level is insufficient.
Methods
Our study was carried out as a case and control and included 3 groups (80 diabetic obese, 80 non-diabetic obese, and 80 healthy individuals as the control group). The Real-Time-PZR(RT-qPZR), and DNA Sanger-Sequencing Methods were used for gene expression and gene squences.
Results
As a result of the analyses, TRAIL gene expression level was found to be higher in the controls than in the diabetic-obese and non-diabetic-obese group. This change in TRAIL gene expression suggests that TRAIL maybe a protective factor against diabetes.
Conclusions
The presence of rs781673405, rs143353036, rs1244378045, rs767450259, rs759369504, rs750556128, and rs369143448 mutations, which was determined with the Sequencing-Method, was shown for the first time in the present study. In addition, it is the first study in which human TRAIL gene-expression and sequencing were performed together. We believe that these data will make an important contribution to the literature.
Theoretical investigations on CL‑20/ANTA cocrystal explosive via molecular dynamics method
Authors: Yu Zhihong , Song Xiaolan , Wang Yi , Cheng Zhipeng , An Chongwei 
Published: June 2023

Abstract

Context
In this study, we developed structural models of hexanitrohexaazaisowurtzitane (CL-20)/3-amino-5-nitro-1,2,4-triazole (ANTA) with different molar ratios ranging from 4:1 to 1:4. We employed a combination of density functional theory and molecular dynamics simulations to investigate the surface electrostatic potential, binding energy, initiation bond length, radial distribution function, cohesive energy density, and mechanical properties of CL-20 and ANTA molecules, as well as the aforementioned models. Additionally, we utilized EXPLO-5 software to predict the detonation properties and products of pure CL-20, ANTA, and CL-20/ANTA systems. The results revealed that there was an interaction between CL-20 and ANTA molecules, which had the potential to form a cocrystal. The most likely molar ratio for cocrystal formation was 1:1, and the main driving forces for cocrystal formation were electrostatic force, dispersion force, and van der Waals force. The cocrystal exhibited moderate sensitivity and excellent mechanical properties. Furthermore, the cocrystal detonation performance at a molar ratio of 1:1 was between that of CL-20 and ANTA, representing a new type of insensitive high-energy material.
Methods
The properties of CL-20/ANTA cocrystal were predicted by molecular dynamics (MD) method under Materials Studio software. For the whole MD simulations, set the temperature at 298 K, and the pressure was 0.0001 GPa. Carried out MD simulation under the NPT ensemble with a total simulation time of 1 ns. The first 0.5 ns was used for thermodynamic equilibrium, and the last 0.5 ns was used for statistical calculation and analysis. Sampling was recorded every 10 fs during the calculation.
Free perforation during the first episode of acute diverticulitis: can it be avoided?
Authors: Agúndez María Carmona , Salamanca Isabel María Gallarín , Martínez Jesús Salas 
Published: June 2023

Abstract
Purpose: The aims of the present study are to compare the severity of acute diverticulitis in the first episode and in later bouts, and to determine whether there are risk factors for the presence of free perforation at the onset of diverticulitis.
Methods: Single-center retrospective study of patients who developed a first episode of acute diverticulitis between January 2011 and August 2021 diagnosed by computed tomography and followed up for at least one year. Free perforation was considered to be present in patients with diverticulitis stage III and IV according to the Hinchey classification (modified by Wasvary).
Results: The analysis included 394 patients (224 men and 170 women) with a mean age of 58 years. Forty-eight patients (12.2%) presented free perforation at some point in the course of the disease, 43 during the first episode and five during subsequent bouts. Perforation group this subgroup presented higher rates of stoma creation and mortality (relative risks of 12.3 and 23.5 respectively). In the multivariate analysis, age (OR: 1.041 95% CI: 1.016-1.067), lung disease (OR 2.154 95% CI: 1.038-4.472) and immunosuppression (OR: 2.812 95% CI: 1.315-6.015) were independent factors for free perforation at diverticulitis onset.
Conclusions: Free perforation occurs more frequently during the first episode of acute diverticulitis. Older patients, immunosuppressed patients and those with respiratory disease have a greater risk of presenting freely perforated diverticulitis. Therefore, it is essential to maintain a high level of clinical suspicion in these patients in order to activate early focus control and thus avoid fatal outcomes.
Evaluation of thermodynamic model of Pd(II) complex formation with isosaccharinic acid
Authors: Kimuro Shingo , Taneichi Yayoi , Iwata Hajime , Ishidera Takamitsu , Kitamura Akira , Tachi Yukio , Tanaka Takeru , Hirano Kana , Hieda Manami , Miyabe Shunsuke , Kawamoto Daisuke 
Published: June 2023

Abstract
Palladium-107 is one of the selected radionuclides in the safety assessment of geological disposal of radioactive waste. Although isosaccharinic acid (ISA) forms strong complexes with many elements and enhances element solubility, the thermodynamic evaluation of the complex of Pd with ISA has not been conducted. In this study, the solubility of Pd(OH)2 at pH 8.5–12.5 in the presence of ISA was investigated under inert gas (N2) atmosphere. Furthermore, the coordination state of aqueous Pd and ISA was investigated by X-ray absorption and Fourie transform infrared spectroscopy, respectively. According to experimental results, the chemical form of the Pd-ISA complex depends on the solution pH; specifically, the number of involved OH− ions in the complex should be changed. The thermodynamic model and conditional equilibrium constants of the Pd-ISA complex were estimated by slope analyses of solubility experiments at different pH levels and ionic strengths based on the specific ion interaction theory. Hence, the impact of complexation with ISA on Pd(II) solubility under disposal conditions could be quantified using the proposed thermodynamic models in this study.
Effect of the neurodynamic mobilization technique combined with electroacupuncture on functional recovery and GAP-43 expression after sciatic nerve injury in rabbits
Authors: Wang Yan , Zheng Linlin , Zhu Luwen , Chen Huijie , Zhang Li , Pei Fei , Liu Bo , Zhao Bin , Wang Yan , Jin Longtao 
Published: June 2023

Abstract
Background
While the peripheral nervous system has remarkable regenerative capacities following injury and disease, its regeneration is often incomplete and slow, and full functional recovery from spontaneous peripheral nerve repair is rare. Hence, there is intense interest in developing a novel strategy for improving functional recovery after peripheral nerve injury (PNI).
Objective
We aimed to determine whether the application of the neurodynamic mobilization technique (NMT) combined with electroacupuncture (EA) can upregulate the expression of proteins relevant to nerve regeneration, such as the GAP-43 protein, as well as enhance functional recovery after sciatic nerve crush injury in rabbits.
Methods
This study establishes a model of a single unilateral crush injury to the sciatic nerve in rabbits. A total of 90 male and female New Zealand rabbits were randomly divided into five groups: the sham injury control group, sciatic nerve crush control (SNCr) group, NMT group, EA group, and NMT + EA group. The function of the injured side of the sciatic nerve was evaluated with the toe-spreading reflex and the modified Tarlov score at the 1-, 2-, and 4-week time points after surgery. We used immunohistochemical analysis and toluidine blue staining to detect changes in the injured sciatic nerve samples.
Results
We found that combination therapy with NMT and EA enhanced locomotor function. Additionally, light microscopy showed that the number of myelinated axons was significantly higher in rabbits treated with a combination of NMT and EA than in rabbits treated with NMT or EA alone. Furthermore, cotreatment with NMT and EA promoted GAP-43 expression in injured neural tissue.
Conclusions
We propose that combination treatment with NMT and EA promotes axon regeneration and functional recovery via the upregulation of GAP-43 expression at the lesion site. Therefore, cotreatment with NMT and EA might be a very promising therapeutic strategy to improve the clinical outcomes of sciatic nerve injury.
Increased extracellular matrix stiffness regulates myofibroblast transformation through induction of autophagy-mediated Kindlin-2 cytoplasmic translocation
Authors: Zhao Zhihan , Han Wuyue , Huang Guotao , He Yong , Zuo Xiaohu , Ning Minqi , Jiang Nuo , Hong Hong 
Published: June 2023

Abstract
The extracellular matrix (ECM) provides structural support for connective tissue and environmental cues for cells. Its mechanical properties regulate biological processes, such as fibroblast-myofibroblast transformation (FMT), which is a crucial component in pelvic organ prolapse (POP) development and leads to an increase in ECM stiffness. The ‘Kindlin-2’ protein, expressed by fibroblasts, plays an important role in the development of the mesoderm, which is responsible for smooth muscle, blood vessel, and connective tissue formation; however, the role of Kindlin-2 in FMT remains to be explored. In this study, we aimed to explore the role of Kindlin-2 in FMT during POP development. In our study, fibroblasts were cultured using gels of different stiffness. We also examined the expression of Kindlin-2 and genes related to the Hippo pathway and FMT, validating them in human tissues and animal models. We found that ECM stiffness induces autophagy to translocate Kindlin-2 to the cytoplasm of L929 cells, where it interacts with and degrades MOB1, thereby facilitating Yes-associated protein (YAP) entry into the nucleus and influencing FMT progression. Stiffness-induced autophagy was inhibited when using an autophagy inhibitor, which blocked the translocation of Kindlin-2 to the cytoplasm and partially reversed high-stiffness-induced FMT. We found that the fibrosis inducer TGF-β failed to induce FMT after Kindlin-2 knockdown, suggesting that Kindlin-2 is involved in the TGF-β-Smad fibrosis axis. In patients with POP, we observed an increase in cytoplasmic Kindlin-2 and nuclear YAP levels. Similar changes in vaginal wall-associated proteins were observed in a mouse model of acute vaginal injury. Overall, Kindlin-2 is a key gene in ECM stiffness, regulating FMT through autophagy induction. Thus, the inhibition of Kindlin-2 transfer to the cytoplasm could be a potential target for the treatment of POP.
Insights into growth, gonadal development, and igf1 expression in captive false clown anemonefish (Amphiprion ocellaris): Implications for breeding programs and sexual development
Authors: Suanla Santi , Thamnawasolos Jeeranan , Boonphakdee Chuta , Shinn Andrew P. 
Published: June 2023

Abstract

Understanding the interplay between growth and gonadal development is crucial for breeding programs and teleost sexual development. Here the growth and gonadal development of captive-reared false clown anemonefish (Amphiprion ocellaris) aged one to eight months was investigated. Histological analysis and quantification of insulin-like growth factor-1 (igf1) mRNA transcripts were performed. The fish displayed a maximum specific growth rate of 0.56 ± 0.11% day− 1 at two months with a negative allometric length-weight relationship. Gonadal differentiation occurred at three months, with concurrent oogenesis and spermatogenesis at four months. Male germ cell development intensified from five to eight months, at the stage of perinucleolus oocytes were observed. Expression levels of igf1 in the liver peaked at eight months; expression was highest in gonadal tissues at two months and decreased significantly in older fish. These findings highlight the potential role of igf1 in ovarian differentiation and the growth of primary oocytes. Further investigations are needed to explore the interplay between igf1 expression and other regulatory factors. This research enhances our understanding of fish reproductive biology and has implications for the captive management of false clownfish.
Foxo3 knockdown mediates decline of Myod1 and Myog reducing myoblast conversion to myotubes
Authors: Gellhaus Benjamin , Böker Kai O. , Gsaenger Marlene , Rodenwaldt Eyck , Hüser Marc A. , Schilling Arndt F. , Dominik Saul 
Published: June 2023

Abstract
Background
Sarcopenia has a high prevalence among the aging population. Leading to falls and hospitalization, subsequently increasing healthcare costs while limiting the quality of life, sarcopenia is of tremendous socioeconomic importance. In sarcopenic muscle fibers, the E3 ubiquitin ligase F-Box Protein 32 (Fbxo32) is expressed at substantially higher levels, driving ubiquitin-proteasomal muscle protein degradation. As one of the key regulators of muscular equilibrium, the transcription factor Forkhead Box O3 (FOXO3) can increase the expression of Fbxo32, making it a possible target for regulation of this detrimental pathway.
Methods
To test this hypothesis, murine C2C12 myoblasts were transduced with AAVs carrying a plasmid for four specific siRNAs against Foxo3. Successfully transduced myoblasts were selected via FACS cell sorting to establish single clone cell lines. Sorted myoblasts were further differentiated into myotubes and stained for myosin heavy chain (MHC) by immunofluorescence. The resulting area was calculated. Myotube contractions were induced by electrical stimulation and quantified.
Results
We found an increased Foxo3 expression in satellite cells in human skeletal muscle and an age-related increase in Foxo3 expression in older mice in silico. We established an in vitro AAV-mediated FOXO3 knockdown on protein level. Surprisingly, the myotubes with FOXO3 knockdown displayed a smaller myotube size and a lower number of nuclei per myotube compared to control myotubes (AAV-transduced with a functionless control plasmid). During differentiation, a lower level of FOXO3 reduced the expression Fbxo32 within the first three days. Moreover, the expression of Myod1 and Myog via ATM and Tp53 was reduced. Functionally, the Foxo3 knockdown myotubes showed a higher contraction duration and time to peak.
Conclusions
Early Foxo3 knockdown seems to terminate the initiation of differentiation due to lack of Myod1 expression and mediates the inhibition of Myog. Subsequently, the myotube size is reduced and the excitability to electrical stimulation is altered.
Identifying dimensions and weighting for Poverty and Well-being Measurements through natural language of people.
Authors: Muñetón-Santa Guberney , Orozco-Arroyave Juan Rafael 
Published: June 2023

Abstract
Purpose: Poverty and well-being indexes are composed of valuable aspects of life that are worth measuring, commonly referred to as dimensions. The objective of this study is to identify dimensions of poverty and well-being based on the natural language used by individuals. Additionally, we propose a methodology for extracting dimensional weights to assess indicators based on people’s concerns.
Methods: Through the application of various topic modeling techniques within the Natural Language Processing approach, we have identified significant variables or dimensions related to poverty and well-being. We have implemented transfer learning using a zero-shot classification model to determine weights for the identified dimensions.
Results: In the specific cases examined in this study, the most important dimensions of poverty are associated with: lack of opportunities, employment, lack of spirit, money, and progress; while in the context of well-being, the top four dimensions identified are: living well, basic needs, being nourished, and health.
Conclusion: By using our semi-automatic methodology, which incorporates natural language processing, we offer a valuable contribution to the literature, enabling the identification of variables and the allocation of weights for constructing comprehensive social indicators. We recommend the use of topic modeling techniques for identifying dimensions in multidimensional wellbeing and poverty indicators, as they empower individuals by providing them with a platform to voice their concerns and ensuring their representation when designing and evaluating social indicators.
The human pathogen, Schistosoma mansoni, lacks the cognate sequence for human telomerase reverse transcriptase (hTERT) and relies on the snail host, Biomphalaria glabrata homologous enzyme for its intra-molluscan development.
Authors: Knight Mathilde , Pels Nana , Yadav Swara , Elhelu Oumsalama , Parn Simone , Rinaldi Gabriel , Grigoriadis Dionysios , Mann Victoria , Brindley Paul , Bridger Joanna 
Published: June 2023

Abstract
The human telomerase reverse transcriptase (hTERT) is the catalytic sub-unit of the ribonuclear protein, telomerase. Together with telomerase RNA, the enzyme complex participates in the maintenance of telomeres at the proximal ends of chromosomes, adding species-specific repeats to the 3’end of the telomere. The regulation of hTERT is tightly linked to the cell cycle and cell differentiation states governing either malignancy or senescence, making it a prospective therapeutic target of cell proliferation in cancer. Malignancy behaves like a parasitic disease in that it only progresses by depending on biochemical and molecular pathways of the host. The snail host/schistosome relationship provides a facile model to examine the regulation of the cancer transcriptome, such as the gastropod homolog of hTERT. To test this hypothesis in relation to the development of larval Schistosoma mansoni in the Biomphalaria glabrata, we utilized an in-silico approach to identify the hTERT homolog of B. glabrata. The human hTERT amino acid sequence (ID 014746) shows a strong homology (E-value of 2e86) to the B. glabrata ortholog (733 amino acids, accession XP_013074763.1). BLASTp analyses using S. mansoni as the query suggested that the parasite lacks a cognate TERT. To study the regulation of the snail-like hTERT in relation to schistosome development, transcriptome analysis was performed which revealed a temporal regulation of the telomerase before and during S. mansoni infection, with an upregulation of B. glabrata hTERT transcription evident by 30 minutes after exposure to the parasite. The anti-telomerase drugs, BPPA and BIBR at 100 ng/mL before infection blocked shedding of parasite cercariae. These findings indicate that the schistosome may rely on the telomerase of its host for asexual reproduction, development and proliferation.
Analysis of 2D Photonic crystal based all optical comparator
Authors: Maji Kajal , Mukherjee Kousik , Mandal Mrinal Kanti 
Published: June 2023

Abstract

In this article we have presented all optical comparator using 2-D photonic crystal of silicon poles with dimensions 10a×12a. The structure of this device is very simple having small footprint of nearly 62 µm2. All-optical comparator has been validated by employing finite-difference-time-domain (FDTD) method. We have analyzed this device to calculate the ER, CR and Q value and verified the output by optical field distribution and amplitude with frequency. The performance of the all optical comparator has been investigated by calculating the variation of ER,CR, Q and transmission ratio with wavelength of the input signal and rod radius.
Region-specific lifestyle and subjective perception are associated with oral health-related quality of life among Tibetan adolescents: a cross-sectional study
Authors: Duan Shaoying , Tang Renjie , Zhang Chenchen , Su Qianqian , Yang Huiyu , Cai He , Hu Tao 
Published: June 2023

AbstractBackground Research on the predictors of oral health-related quality of life (OHRQoL) in Tibetan adolescents is limited. The aim of this study was to investigate the OHRQoL of Tibetan adolescents in Ganzi, Sichuan, and to determine whether the region-specific lifestyle, subjective perception and objective conditions of oral health could predict Tibetan adolescents’ OHRQoL. Methods A cross-sectional survey of Tibetan adolescents aged 11–15 years was conducted in Ganzi, Sichuan. Relevant data was collected via a self-reported questionnaire and a complete oral examination. The OHRQoL was measured using the standardized Child Oral Impacts on Daily Performances (sC-OIDP) scores. Binary logistic regression was used to explore the association between region-specific lifestyle, subjective perception, objective conditions and OHRQoL. Results Four hundred and eighty-five Tibetan adolescents participated in the study. The sC-OIDP ranged from 39.44 to 99.70 (mean55.41 ± SD11.19) with 288 (59.38%) adolescents reported an sC-OIDP above 50. It was revealed that Tibetan adolescents living at a higher altitude of 3300m than 1400m were more likely to report a poorer OHRQoL (OR = 1.880, 95%CI 1.101–3.209,P = .021). Also, buttered tea consumption by adolescents (OR = 2.284, 1.200-4.348,P = .012) or by mother (OR = 2.278, 1.171–4.432,P = .015) and boarding student (OR = 1.761, 1.180–2.626,P = .006) were significantly associated with poorer OHRQoL. Regarding the subjective perception, adolescents that rated a bad or very bad oral health (OR = 4.360, 2.341–8.120,P < .001) was more likely to have poorer OHRQoL compared to those with good or very good oral health. Furthermore, self-reported dental pain was significantly related to poorer OHRQoL (OR = 2.836, 1.836–4.382,P < .001). Interestingly, objective conditions, such as BOP, DC and DMFT were not related to OHRQoL. Conclusion Region-specific lifestyle and subjective perception on oral health were the key predictors affecting the OHRQoL of Tibetan adolescents. To improve OHRQoL, it is recommended to weigh the pros and cons of buttered tea and take moderate consumption among adolescents and their mothers and improve communication of students in boarding school with their parents can boost self-confidence. Additionally, developing good oral hygiene habits and keeping more healthy teeth to prevent dental pain is equally important for improving OHRQoL.