bluuebunny/crossref_metadata_2025_split huggingface dataset:
The joy of the 11th International Fission Yeast Meeting in Hiroshima (POMBE2023 Hiroshima) after a long wait due to the COVID‐19 pandemic
Authors: Toda Takashi , Kitamura Kenji , Kume Kazunori , Yukawa Masashi , Koyano Takayuki , Ueno Masaru 
Published: July 2023

AbstractThe 11th International Fission Yeast Meeting took place at Astel Plaza in Hiroshima, Japan, from May 28th to June 2nd, 2023. This highly anticipated gathering, originally scheduled for May 2021, had been postponed for 2 years due to the COVID‐19 pandemic. Researchers from 21 countries, including 211 overseas and 157 domestic participants (overall gender ratio is roughly 60% male vs. 40% female), eagerly awaited the opportunity to meet in person, as virtual interactions had been the only means of communication during this challenging period. The meeting featured four kick‐off special lectures, 101 regular talks, and 152 poster presentations. Additionally, a discussion session on upfront frontier research in fission yeast provided an interactive platform for both speakers and attendees. Throughout the event, participants shared cutting‐edge knowledge, celebrated significant research findings, and relished the invaluable experience of an in‐person meeting. The vibrant and friendly atmosphere, characteristic of this esteemed international conference, fostered collaboration and reinforced the significance of studying this exceptional model organism. Undoubtedly, the outcomes of this meeting will greatly contribute to our understanding of complex biological systems, not only in fission yeast but also in general eukaryotes.
Formation mechanism of TiC/Al2O3 composite from porous Ti3AlC2 ceramics in 35 vol.% CO‐N2 atmosphere
Authors: Xiao Junli , Wei Yaowu , Peng Conghua , Zhou Siqi , Zhang Shaowei , Li Nan , Chen Junfeng 
Published: July 2023

AbstractThermal reaction and microstructure evolution of porous Ti3AlC2 ceramics in a graphite bed (65% N2 and 35% CO) between 1100 and 1500°C were investigated in this work. The main products of Ti3AlC2 in a graphite bed were titanium carbide and alumina. At 1100°C, a thin alumina layer formed on the Ti3AlC2 particles due to the outward diffusion of slight aluminum. As the temperature increased to 1300°C, more aluminum diffused outward from the Ti3AlC2 particles, and a partial of Ti3AlC2 transformed into titanium carbide. Consequently, the alumina layer formed on the surface of particles got thicker, and defects occurred inside the particles due to the Kirkendall effect. When the temperature was 1500°C, Ti3AlC2 was transformed entirely into alumina and titanium carbide, and visible pores were left inside the particles. The formation mechanism of TiC/Al2O3 composite from porous Ti3AlC2 ceramics in a graphite bed was proposed in detail.
Air‐breathing behavior in Heterotis niloticus fingerlings: Response to changes in oxygen, temperature, and exercise regimes
Authors: Adjei Henry Owusu , Laar Robert Yadama , Ofori‐Darkwah Prince , Xatse Emmanuel Xorla , Bediako Jedida Osei , Skov Peter Vilhelm , Obirikorang Kwasi Adu 
Published: July 2023

AbstractAir‐breathing in fish is believed to have arisen as an adaptation to aquatic hypoxia. Although air‐breathing has been widely studied in numerous fish species, little is known about the obligate air‐breathing African bonytongue, Heterotis niloticus. We evaluated if abiotic factors and physical activity affect air‐breathing patterns in fingerlings. The air‐breathing frequency (fAB) and behavioral responses of H. niloticus fingerlings were assessed in response to environmental oxygen, temperature, and exhaustion and activity in a series of experiments. The air‐breathing behavior of H. niloticus fingerlings under optimum water conditions was characterized by swift excursions lasting less than 1 s to the air–water interface to gulp air. The intervals between air‐breaths were highly variable, ranging from 3 to 259 s. Body size only slightly affected fAB, while hypoxia, hyperthermia, and exercise stress significantly increased fAB. Progressive hypoxia from 17.69 to 2.17 kPa caused a ~2.5‐fold increase in fAB. Increasing temperatures to 27 and 32°C, from a baseline temperature of 22°C, significantly increased fAB from 0.4 ± 0.2 to 1.3 ± 0.5 and 1.6 ± 0.4 breaths min−1, respectively. Lastly, following exhaustive exercise, fAB increased up to 3‐fold. These observations suggest that H. niloticus fingerlings are very reliant on aerial oxygen, and their air‐breathing behavior is sensitive to environmental changes and activity levels.
Disparities in advance care planning among older US immigrants
Authors: Mindo‐Panusis Dallas , Sudore Rebecca L. , Cenzer Irena , Smith Alexander K. , Kotwal Ashwin A. 
Published: July 2023

AbstractBackgroundDespite known racial disparities in advance care planning (ACP), little is known about ACP disparities experienced by US immigrants.MethodsWe used data from the 2016 wave of the Health and Retirement Study. We defined ACP engagement as self‐reported end‐of‐life (EOL) discussions, designation of a power of attorney (DPOA), documented living will, or “any” of the three behaviors. Immigration status was determined by respondent‐reported birth outside the United States. Time in the United States was calculated by subtracting the year of arrival in the United States from the survey year of 2016. We used multivariable logistic regression to estimate the association between ACP engagement and immigration status and the relationship of acculturation to ACP engagement, adjusting for sociodemographics, religiosity, and life expectancy.ResultsOf the total cohort (N = 9928), 10% were immigrants; 45% of immigrants identified as Hispanic. After adjustment, immigrants had significantly lower adjusted probability of any ACP engagement (immigrants: 74% vs. US‐born: 83%, p < 0.001), EOL discussions (67% vs. 77%, p < 0.001), DPOA designation (50% vs. 59%, p = 0.001) and living will documentation (50% vs. 56%, p = 0.03). Among immigrants, each year in the United States was associated with a 4% increase in the odds of any ACP engagement (aOR 1.04, 95% CI 1.03–1.06), ranging from 36% engaged 10 years after immigration to 78% after 70 years.ConclusionACP engagement was lower for US immigrants compared to US‐born older adults, particularly for those that recently immigrated. Future studies should explore strategies to reduce disparities in ACP and the unique ACP needs among different immigrant populations.
Accuracy of equations for estimating resting energy expenditure in children and adolescents living with phenylketonuria
Authors: Mezzomo Thais R. , Dias Marcia Regina Messaggi G. , Vilela Regina M. , Pereira Rosana Marques 
Published: July 2023

AbstractBackgroundMeasuring resting energy expenditure (REE) in individuals living with phenylketonuria (PKU) using indirect calorimetry (IC) is unusual in healthcare facilities because it requires specific protocols and expensive equipment. Considering that determining REE is crucial for devising nutritional strategies for the management of PKU, the aim of this study was to identify the predictive equations that provide the best estimates of REE in children and adolescents living with PKU and to propose a predictive equation for determining REE in this population.MethodsAn REE concordance study was conducted with children and adolescents living with PKU. Anthropometric and body composition assessments using bioimpedance and REE assessment using IC were performed. The results were compared to 29 predictive equations.ResultsFifty‐four children and adolescents were evaluated. The REE obtained using IC differed from all estimated REE, except Henry's equation for male children (p = 0.058). Only this equation showed good agreement (0.900) with IC. Eight variables were associated with the REE obtained using IC with emphasis on fat‐free mass (kg) (r = 0.786), weight (r = 0.775), height (r = 0.759) and blood phenylalanine (r = 0.503). With these variables, three REE equations were suggested, with R2 = 0.660, 0.635 and 0.618, respectively, and the third equation, which involves weight and height, showed adequate sample size for a statistical power of 0.942.ConclusionMost equations, not specific for individuals living with PKU, overestimate the REE of this population. We propose a predictive equation for assessing REE for children and adolescents living with PKU to be used in settings where IC is not available.
Recent advances in cholinergic mechanisms as reactions to toxicity, stress, and neuroimmune insults
Authors: Kovarik Zrinka , Moshitzky Gilli , Maček Hrvat Nikolina , Soreq Hermona 
Published: July 2023

AbstractThis review presents recent studies of the chemical and molecular regulators of acetylcholine (ACh) signaling and the complexity of the small molecule and RNA regulators of those mechanisms that control cholinergic functioning in health and disease. The underlying structural, neurochemical, and transcriptomic concepts, including basic and translational research and clinical studies, shed new light on how these processes inter‐change under acute states, age, sex, and COVID‐19 infection; all of which modulate ACh‐mediated processes and inflammation in women and men and under diverse stresses. The aspect of organophosphorus (OP) compound toxicity is discussed based on the view that despite numerous studies, acetylcholinesterase (AChE) is still a vulnerable target in OP poisoning because of a lack of efficient treatment and the limitations of oxime‐assisted reactivation of inhibited AChE. The over‐arching purpose of this review is thus to discuss mechanisms of cholinergic signaling dysfunction caused by OP pesticides, OP nerve agents, and anti‐cholinergic medications; and to highlight new therapeutic strategies to combat both the acute and chronic effects of these chemicals on the cholinergic and neuroimmune systems. Furthermore, OP toxicity was examined in view of cholinesterase inhibition and beyond in order to highlight improved small molecules and RNA therapeutic strategies and assess their predicted pitfalls to reverse the acute toxicity and long‐term deleterious effects of OPs.image
How local, first‐line nurse leaders can positively influence nurse intent to stay and retention: A realist review
Authors: Cardiff Shaun , Gershuni Olga , Giesbergen‐Brekelmans Anne 
Published: July 2023

AbstractAims and ObjectivesTo explore evidence on how local nurse leaders can positively influence nurse retention.BackgroundNurse turnover and retention is a wicked problem with multiple interrelated factors and no one solution. Local nurse leadership has the potential to positively influence nurse intent to stay directly or via various factors.DesignA realist review.MethodsHaving designed a search strategy based on a tentive programme theory, 1386 initial hits in three databases were screened and reduced to 48 research articles published between 2010–2021. The articles' content were coded for findings that supported, refined or contradicted four ContextMechanismOutcome configurations.ResultsSufficient evidence was found to support four guiding lights encouraging local nurse leaders to: (1) foster relational connectedness; (2) enable professional practice autonomy; (3) cultivate healthful workplace cultures; (4) support professional growth and development. Mutuality and reciprocity is also key if leaders themselves are to experience wellbeing and growth.ConclusionsPerson‐centred, transformational and resonant local nurse leaders can positively influence nurse intent to stay within the workplace or organisation.Reporting MethodConform JCN guideline ‘What to cover in the main tekst by article type’ for reviews.Patient or Public ContributionNo Patient or Public Contribution.
Depletion of circ_0088046 suppressed cell growth and motility of hepatocellular carcinoma via circ_0088046‐miR‐1299‐RTKN2 ceRNA pathway
Authors: Zhang Li , He Sai , Guan Hao , Zhao Yao , Zhang Di 
Published: July 2023

AbstractCircular RNAs (circRNAs) have been verified to be important modulators and therapeutic targets of human hepatocellular carcinoma (HCC). This study aims to explore the role and mechanism of circ_0088046 in HCC progression. Quantitative real‐time polymerase chain reaction (qRT‐PCR), western blot and immunohistochemistry assays were used to detect the mRNA and protein expression of circ_0088046, miR‐1299, Rhotekin 2 (RTKN2), Bax, Bcl‐2, E‐cadherin and Ki‐67. Cell proliferation was investigated by 5‐Ethynyl‐2′‐deoxyuridine (EdU) assay and cell colony formation assay. Cell apoptosis rate was measured by flow cytometry. Transwell migration and invasion assays were adopted to assess cell migration and invasion. The molecular target relationship between miR‐1299 and circ_0088046 or RTKN2 were analysed by dual‐luciferase reporter assay and RNA immunoprecipitation assay. An animal experiment was conducted to demonstrate the effect of circ_0088046 on tumour formation in vivo. High levels of circ_0088046 and RTKN2, and low levels of miR‐1299 were displayed in HCC tissues and cells. Circ_0088046 absence repressed cell proliferation, migration and invasion, but boosted apoptosis of HCC cells. MiR‐1299 was a target of circ_0088046 and miR‐1299 inhibitor restored circ_0088046 silencing‐mediated inhibitory impacts on HCC cell malignancy. MiR‐1299 could directly target RTKN2, and overexpressed RTKN2 rescued the suppressive effects caused by miR‐1299 mimic. In addition, circ_0088046 silencing constrained tumour formation in vivo. Circ_0088046 contributed to HCC cell malignancy via modulating the miR‐1299/RTKN2 axis.
Carbon starvation following a decade of experimental drought consumes old reserves in Pinus edulis
Authors: Peltier Drew M. P. , Carbone Mariah S. , McIntire Cameron D. , Robertson Nathan , Thompson R. Alex , Malone Shealyn , LeMoine Jim , Richardson Andrew D. , McDowell Nate G. , Adams Henry D. , Pockman William T. , Trowbridge Amy M. 
Published: July 2023

Summary
Shifts in the age or turnover time of non‐structural carbohydrates (NSC) may underlie changes in tree growth under long‐term increases in drought stress associated with climate change. But NSC responses to drought are challenging to quantify, due in part to large NSC stores in trees and subsequently long response times of NSC to climate variation.
We measured NSC age (Δ14C) along with a suite of ecophysiological metrics in Pinus edulis trees experiencing either extreme short‐term drought (−90% ambient precipitation plot, 2020–2021) or a decade of severe drought (−45% plot, 2010–2021). We tested the hypothesis that carbon starvation – consumption exceeding synthesis and storage – increases the age of sapwood NSC.
One year of extreme drought had no impact on NSC pool size or age, despite significant reductions in predawn water potential, photosynthetic rates/capacity, and twig and needle growth. By contrast, long‐term drought halved the age of the sapwood NSC pool, coupled with reductions in sapwood starch concentrations (−75%), basal area increment (−39%), and bole respiration rates (−28%).
Our results suggest carbon starvation takes time, as tree carbon reserves appear resilient to extreme disturbance in the short term. However, after a decade of drought, trees apparently consumed old stored NSC to support metabolism.
A promoter trap in transgenic citrus mediates recognition of a broad spectrum of Xanthomonas citri pv. citriTALEs, including in planta‐evolved derivatives
Authors: Shantharaj Deepak , Minsavage Gerald V. , Orbović Vladimir , Moore Gloria A. , Holmes Danalyn R. , Römer Patrick , Horvath Diana M. , Lahaye Thomas , Jones Jeffrey B. 
Published: July 2023

SummaryCitrus bacterial canker (CBC), caused by Xanthomonas citri subsp. citri (Xcc), causes dramatic losses to the citrus industry worldwide. Transcription activator‐like effectors (TALEs), which bind to effector binding elements (EBEs) in host promoters and activate transcription of downstream host genes, contribute significantly to Xcc virulence. The discovery of the biochemical context for the binding of TALEs to matching EBE motifs, an interaction commonly referred to as the TALE code, enabled the in silico prediction of EBEs for each TALE protein. Using the TALE code, we engineered a synthetic resistance (R) gene, called the Xcc‐TALE‐trap, in which 14 tandemly arranged EBEs, each capable of autonomously recognizing a particular Xcc TALE, drive the expression of Xanthomonas avrGf2, which encodes a bacterial effector that induces plant cell death. Analysis of a corresponding transgenic Duncan grapefruit showed that transcription of the cell death‐inducing executor gene, avrGf2, was strictly TALE‐dependent and could be activated by several different Xcc TALE proteins. Evaluation of Xcc strains from different continents showed that the Xcc‐TALE‐trap mediates resistance to this global panel of Xcc isolates. We also studied in planta‐evolved TALEs (eTALEs) with novel DNA‐binding domains and found that these eTALEs also activate the Xcc‐TALE‐trap, suggesting that the Xcc‐TALE‐trap is likely to confer durable resistance to Xcc. Finally, we show that the Xcc‐TALE‐trap confers resistance not only in laboratory infection assays but also in more agriculturally relevant field studies. In conclusion, transgenic plants containing the Xcc‐TALE‐trap offer a promising sustainable approach to control CBC.
PSW1, an LRR receptor kinase, regulates pod size in peanut
Authors: Zhao Kunkun , Wang Long , Qiu Ding , Cao Zenghui , Wang Kuopeng , Li Zhan , Wang Xiaoxuan , Wang Jinzhi , Ma Qian , Cao Di , Qi Yinyao , Zhao Kai , Gong Fangping , Li Zhongfeng , Ren Rui , Ma Xingli , Zhang Xingguo , Yu Feng , Yin Dongmei 
Published: July 2023

SummaryPod size is a key agronomic trait that greatly determines peanut yield, the regulatory genes and molecular mechanisms that controlling peanut pod size are still unclear. Here, we used quantitative trait locus analysis to identify a peanut pod size regulator, POD SIZE/WEIGHT1 (PSW1), and characterized the associated gene and protein. PSW1 encoded leucine‐rich repeat receptor‐like kinase (LRR–RLK) and positively regulated pod stemness. Mechanistically, this allele harbouring a 12‐bp insertion in the promoter and a point mutation in the coding region of PSW1 causing a serine‐to‐isoleucine (S618I) substitution substantially increased mRNA abundance and the binding affinity of PSW1 for BRASSINOSTEROID INSENSITIVE1‐ASSOCIATED RECEPTOR KINASE 1 (BAK1). Notably, PSW1HapII (super‐large pod allele of PSW1) expression led to up‐regulation of a positive regulator of pod stemness PLETHORA 1 (PLT1), thereby resulting in larger pod size. Moreover, overexpression of PSW1HapII increased seed/fruit size in multiple plant species. Our work thus discovers a conserved function of PSW1 that controls pod size and provides a valuable genetic resource for breeding high‐yield crops.
Cardiac interoception in infants: Behavioral and neurophysiological measures in various emotional and self‐related contexts
Authors: Weijs Marieke L. , Daum Moritz M. , Lenggenhager Bigna 
Published: July 2023

AbstractInteroception, the perception of internal bodily signals, is fundamental to our sense of self. Even though theoretical accounts suggest an important role for interoception in the development of the self, empirical investigations are limited, particularly in infancy. Previous studies used preferential‐looking paradigms to assess the detection of sensorimotor and multisensory contingencies in infancy, usually related to proprioception and touch. So far, only one recent study reported that infants discriminated between audiovisual stimuli presented synchronously or asynchronously with their heartbeat. This discrimination was related to the amplitude of the infant's heartbeat evoked potentials (HEP), a neural correlate of interoception. In the current study, we measured looking preferences between synchronous and asynchronous visuocardiac (bimodal), and audiovisuocardiac (trimodal) stimuli as well as the HEP in conditions of different emotional contexts and with different degrees of self‐relatedness in a mirror‐like setup. While the infants preferred trimodal to bimodal stimuli, we did not observe the predicted differences between synchronous and asynchronous stimulation. Furthermore, the HEP was not modulated by emotional context or self‐relatedness. These findings do not support previously published results and highlight the need for further studies on the early development of interoception in relation to the development of the self.
Zombies Incorporated
Authors: Leffler Olof 
Published: July 2023

AbstractHow should we understand the relation between corporate agency, corporate moral agency and corporate moral patienthood? For some time, corporations have been treated as increasingly ontologically and morally sophisticated in the literature. To explore the limits of this treatment, I start off by redeveloping and defending areductiothat historically has been aimed at accounts of corporate agency which entail that corporations count as moral patients. More specifically, I argue that standard agents are due a certain type of moral concern, but corporate agents are not due that type of concern, so they are not agents of, at least, the standard type. Diagnosis: because corporations plausibly lack qualia, they are ‘zombie agents’ that mimic real agents. This explains why they are neither standard agents nor standard moral patients: they are not standard agents because they lack mental states, and because they lack experiences, they are, at best, morally notable rather than morally respectable. However, I then argue that we nevertheless have instrumental reasons to include them in our moral responsibility practices, both because that is what notability involves and bluffingly, and both when it comes to treating them as moral agents and as moral patients. Hence, we may treat corporations as part of our moral responsibility practices to a limited extent anyway.