bluuebunny/crossref_metadata_2025_split huggingface dataset:
Uptake and anti-inflammatory effects of liposomal astaxanthin on endothelial cells tracked by Raman and fluorescence imaging
Authors: Radwan Basseem , Prabhakaran Amrutha , Rocchetti Stefano , Matuszyk Ewelina , Keyes Tia E. , Baranska Malgorzata 
Published: July 2023

Abstract
Astaxanthin (AXT) is a lipophilic antioxidant and anti-inflammatory natural pigment whose cellular uptake and bioavailability could be improved via liposomal encapsulation. Endothelial cells (EC) line the lumen of all blood vessels and are tasked with multiple roles toward maintaining cardiovascular homeostasis. Endothelial dysfunction is linked to the development of many diseases and is closely interconnected with oxidative stress and vascular inflammation. The uptake of free and liposomal AXT into EC was investigated using Raman and fluorescence microscopies. AXT was either encapsulated in neutral or cationic liposomes. Enhanced uptake and anti-inflammatory effects of liposomal AXT were observed. The anti-inflammatory effects of liposomal AXT were especially prominent in reducing EC lipid unsaturation, lowering numbers of lipid droplets (LDs), and decreasing intercellular adhesion molecule 1 (ICAM-1) overexpression, which is considered a well-known marker for endothelial inflammation. These findings highlight the benefits of AXT liposomal encapsulation on EC and the applicability of Raman imaging to investigate such effects.

Graphical Abstract
BGG Sequences with Weak Regularity and Applications
Authors: Čap Andreas , Hu Kaibo 
Published: July 2023

AbstractWe investigate some Bernstein–Gelfand–Gelfand complexes consisting of Sobolev spaces on bounded Lipschitz domains in $${\mathbb {R}}^{n}$$

R

n

. In particular, we compute the cohomology of the conformal deformation complex and the conformal Hessian complex in the Sobolev setting. The machinery does not require algebraic injectivity/surjectivity conditions between the input spaces, and allows multiple input complexes. As applications, we establish a conformal Korn inequality in two space dimensions with the Cauchy–Riemann operator and an additional third-order operator with a background in Möbius geometry. We show that the linear Cosserat elasticity model is a Hodge–Laplacian problem of a twisted de Rham complex. From this cohomological perspective, we propose potential generalizations of continuum models with microstructures.
Small-scale genetic structure of populations of the bulb mite Rhizoglyphus robini
Authors: Przesmycka Karolina , Radwan Jacek 
Published: July 2023

AbstractBulb mites are an economically significant pest of subterranean parts of plants and a versatile laboratory animal. However, the genetic structure of their populations remains unknown. To fill this gap in our knowledge of their biology, we set up a field experiment in which we allowed mites to colonize onion bulbs, and then determined the genetic structure of colonisers based on a panel of microsatellite loci. We found moderate but significant population structure among sites separated by ca. 20 m (FST range 0.03–0.21), with 7% of genetic variance distributed among sites. Allelic richness within some bulbs was nearly as high as that in the total population, suggesting that colonisation of bulbs was not associated with strong population bottlenecks. The significant genetic structure we observed over small spatial scales seems to reflect limited dispersal of mites in soil.
Mitochondrial DNA diversity of the eastern black rhinoceros (Diceros bicornis michaeli) in Tanzania: implications for future conservation
Authors: Mellya Ronald V. K. , Hopcraft J. Grant C. , Eblate Ernest M. , Kariuki Linus , Otiende Moses , Chuma Idrissa S. , Macha Emmanuel S. , Wambura Dickson , Kilbride Elizabeth , Mable Barbara K. 
Published: July 2023

AbstractThere has been a drastic decline in the number of eastern black rhinoceros (Diceros bicornis michaeli) across Africa, leaving individuals restricted to small, isolated populations that are vulnerable to extinction. Focusing on highly threatened populations in Tanzania, this study investigated the genetic impacts of past management interventions on mitochondrial control region diversity in extant subpopulations, assessed whether there has been evidence of dispersal of females between populations based on haplotype sharing, and related current haplotype diversity to historical patterns. Across extant subpopulations in Tanzania, six maternal haplotypes were identified, with an overall haplotype diversity of h = 0.72 but lower overall nucleotide diversity within populations (π = 0.017) compared to historical populations (π = 0.021). Translocated populations did not share haplotypes with native populations, even though all haplotypes from translocated individuals had been found among historic samples from Kenya. This suggests that translocations have been successful at restoring previous diversity to the region but that the current Intensive Protection Zone (IPZ) management practices have restricted the movement of females between subpopulations. Extant haplotypes were distributed among three East African haplogroups described in previous studies, suggesting that multiple lineages have been preserved despite the loss of historical haplotypes. Our recommendation is to enhance the utilisation of previous translocations by enabling the natural movements of individuals between subpopulations. Such a change in management strategy could be less costly both economically and in terms of animal welfare than the alternative of using genetic data to target specific animals for translocation in order to supplement diversity.
The Landscapes of Disease and Death in Colonial Mauritius
Authors: Cianciosi Alessandra 
Published: July 2023

AbstractThe recurring ebb and flow of epidemic diseases profoundly impacted how colonial administrations dealt with death. This article focuses on the role disease played in shaping the “necrogeography” of colonial landscapes, a key point of intersection between funerary and landscape archaeology. Using an extensive corpus of evidence from cemeteries that capture inhumation practices from formerly enslaved and indentured populations, this article provides an assessment of these burial contexts as part of the cultural landscape in Mauritius. Drawing together functional and emotional dimensions, their features and development will be considered against the backdrop of the island’s specific and dynamic disease ecology.
Ameliorative effect of montelukast against STZ induced diabetic nephropathy: targeting HMGB1, TLR4, NF-κB, NLRP3 inflammasome, and autophagy pathways
Authors: Awad Ahmed M. , Elshaer Sally L. , Gangaraju Rajashekhar , Abdelaziz Rania R. , Nader Manar A. 
Published: July 2023

AbstractDiabetic nephropathy (DN) is reported as one of the most serious microvascular diabetic complications and the trigger of end-stage renal disease (ESRD), underscoring the concern of any therapeutic intervention directed at ameliorating the development and progression of DN. The current study explored the renoprotective impact of montelukast (Mon) against streptozotocin (STZ)-induced DN in rats compared to a standard anti-hyperglycemic insulin (Ins) treatment. Diabetes was induced by a single dose of STZ (55 mg/kg). Diabetic rats were treated with Mon (10 and 20 mg/kg, oral gavage) for eight weeks. Mon administration for 8 weeks after induction of diabetes conferred significant dose-dependent renoprotection, independent of blood glucose levels (unlike Ins), as evidenced by the improvement in serum creatinine, and blood urea nitrogen (BUN), and ameliorated STZ-induced renal necrotic, inflammatory alterations, and renal fibrosis. Additionally, Mon treatment in diabetic rats significantly restored redox hemostasis as evidenced by malondialdehyde (MDA) and total antioxidant capacity (TAC) levels; significantly reduced the renal expression of high mobility group box (HMGB) 1, toll-like receptor (TLR) 4, nuclear factor kappa B (NF-κB) (in the nucleus), NOD-like receptor family pyrin domain containing (NLRP) 3, and interleukin (IL)-1β. Moreover, Mon administration ameliorated the dysregulation in autophagy as evidenced by p62 and microtubule-associated protein 1A/1B-light chain 3 (LC3)-II levels. In conclusion, the renoprotective effect of Mon is potentially associated with its modulatory effect on inflammatory cytokines, antioxidant properties, and autophagy.
Graphical Abstract
Renoprotective effect of montelukast and its underlying pathway: Hyperglycemia and advanced glycation end products (AGEs) stimulate the release of high mobility group box (HMGB) 1 from necrotic and inflammatory cells. HMGB1 is considered as one of the endogenous ligands of toll-like receptor (TLR) 4, and the interaction of HMGB1 with TLR4 results in a subsequent translocation of nuclear factor kappa B (NF-κB) from the cytoplasm into the nucleus inducing an inflammatory response. NF-κB is a key mediator of the priming signal responsible for the activation of NOD-like receptor family pyrin domain containing (NLRP) 3 inflammasome by stimulating the expression of both NLRP3 and pro- interleukin (IL)-1β, which is then converted to IL-1β by to mediate inflammation. NLRP3 can induce reactive oxygen species production, while autophagy inhibits AGEs and NLRP3 accumulation. Montelukast show an inhibitory effect on HMGB1, TLR4, NF-κB, NLRP3, and IL-1β and has autophagy stimulating characteristics indicating its potential renoprotective effect.
A Survey on Smart Wearable Devices for Healthcare Applications
Authors: Alattar Alhassan E. , Mohsen Saeed 
Published: July 2023

AbstractThis paper introduces a survey on recent smart wearable devices used in different fields, especially healthcare applications. A discussion is presented in terms of power supply, wireless technologies, applications, and wearability of these devices. Regarding the power supply, a photovoltaic is the best harvesting energy source, while the best energy storage is a Li-ion battery. Regarding wireless technologies, Bluetooth low energy (BLE) is the best for personal usage, while Wi-Fi has preferred for Internet of Things (IoT) applications. Regarding the applications, medical and non-medical applications can be used. Regarding wearability, smart wearable devices can be positioned on a human hand or arm. Also, wearable sensors can be patched on human skin, besides, they can be placed on clothes, such as a smart suit which is preferred for heavy duties.
Prefrontal hemodynamic features of older adults with preserved visuospatial working memory function
Authors: Lee Tsz-lok , Ding Zihan , Chan Agnes S. 
Published: July 2023

Abstract

Memory decline has been observed in the aging population and is a risk factor for the later development of dementia. Understanding how memory is preserved in older adults has been an important topic. The present study examines the hemodynamic features of older adults whose memory is comparable with that of young adults. In the present study, 45 younger and 45 older adults performed the visual memory task with various difficulty levels (i.e., the items to be remembered), and their cerebral hemodynamics at each level were measured by functional near-infrared spectroscopy (fNIRS). The results showed that older adults exhibited higher activation than younger adults under more difficult but not easier levels. In addition, older adults whose performance is comparable with that of young adults (i.e., being able to remember six items) showed more right-lateralized activation. However, those unable to do so showed more left-lateralized activation. The results suggested that high-performing older adults possess successful compensatory mechanisms by recruiting cognitive resources in a specialized brain region.
Gender-Differential Association Between Loneliness and Alcohol Consumption: a Pooled Analysis of 17,808 Individuals in the Multi-Cohort GESA Consortium
Authors: Johar Hamimatunnisa , Atasoy Seryan , Beutel Manfred , Braehler Elmar , Schomerus Georg , Binder Harald , Peters Annette , Zöller Daniela , Fleischer Toni , Otten Danielle , Kampling Hana , Kruse Johannes , Ladwig Karl-Heinz 
Published: July 2023

AbstractLoneliness is an established risk factor for impaired health. However, the evidence of whether increased alcohol consumption is a coping mechanism to alleviate loneliness for both genders remains sparse. The cross-sectional study included 8898 men and 8910 women (mean age of 56.2 ± 11.5 years) from three population-based cohort studies in Germany (KORA-FF4, GHS, and SHIP. Daily alcohol consumption (g/day) was measured, and risky drinking was identified using gender-specific thresholds (40 g/day for men and 20 g/day for women). Loneliness was assessed by asking if the participants feel lonely. Multivariable regression analyses were employed to examine the association between alcohol use outcomes and loneliness with adjustments for confounders. Women reported feeling lonely more frequently than men (14.8% vs 10.4%). In men, loneliness was positively associated with levels of alcohol consumption (ß = 1.75, SE = 0.76, p = 0.04) and risky drinking (OR = 1.33, 95% CI = 1.07–1.66, p = 0.02) and was even more profound in men with lower educational levels. In women, loneliness was associated with reduced odds of risky consumption (OR = 0.76, 95% CI = 0.60–0.96, p = 0.02) but not with alcohol consumption levels. The findings indicate gender-differential associations of loneliness with increased levels and risky alcohol consumption in men but with decreased risky consumption in women.
Artesunate alleviates 5-fluorouracil-induced intestinal damage by suppressing cellular senescence and enhances its antitumor activity
Authors: Xia Jing , Dai Qian long , He Siyue , Jia Hui-jie , Liu Xian-Guo , Hua Hui , Zhou Min , Wang Xiaobo 
Published: July 2023

Abstract
Background
Colorectal cancer (CRC) is one of the most prevalent diagnosed malignancies and one of the leading causes of cancer-related deaths worldwide. 5-Fluorouracil (5-FU) and its combination regimen are commonly used as primary chemotherapeutic agents for advanced CRC. Intestinal mucositis is one of the most frequent side effects of 5-FU. Artesunate (Arte) is derived from the wormwood plant Artemisia annua. Arte is not only effective against malaria but also diabetes, atherosclerosis, inflammation, and other conditions. The mechanism by which 5-FU damages the intestinal tract is unclear, and there is no standard treatment for diarrhea caused by 5-FU. Therefore, it is critical to discover novel and promising therapeutic drugs for 5-FU side effect treatment.

Methods
The morphology and expression of genes and proteins associated with the aging of HUVECs, HIECs, and intestinal tissues were compared to the those of the control group. The cell lines and tissues were evaluated by SA-β-Gal staining, Western blotting, and RT‒qPCR. HIEC and HCT116 cell viability was assessed in vitro by a CCK-8 assay and in vivo by a subcutaneous tumor mouse assay. Tumor cell proliferation and apoptosis was evaluated by immunohistochemistry.

Results
Here, we report that Arte alleviates the adverse side effects caused by 5-FU in intestinal tissue, and that 5-FU-induced intestinal damage is associated with drug-induced chemical inflammation and an increase in the proportion of senescent cells. Arte decreases the ratio of SA-β-Gal-positive cells and downregulated the expression of aging-related proteins (p53, p16) and aging-related genes (p53, p21). Mechanistically, Arte relieves intestinal injury by inhibiting mTOR expression, which is associated with the regulation of aging. Moreover, Arte suppresses the p38MAPK and NF-κB signaling pathways, which are related to inflammation regulation. In addition, the combined therapy of Arte plus 5-FU significantly decreases cancer cell viability in vitro. Arte and 5-FU synergistically reduce the growth of colorectal cancer (CRC) xenografts in vivo.

Conclusions
Overall, our findings point to the crucial treatment effect of Arte on inflammation, intestinal cell senescence, and CRC cell proliferation and offer a new option for CRC treatment.
Angle Measurement Based on Second Harmonic Generation Using Artificial Neural Network
Authors: Li Kuangyi , Zhang Zhiyang , Lin Jiahui , Sato Ryo , Matsukuma Hiraku , Gao Wei 
Published: July 2023

AbstractThis article proposed an angle measurement method based on second harmonic generation (SHG) using an artificial neural network (ANN). The method comprises three sequential parts: SHG spectrum collection, data preprocessing, and neural network training. First, the referenced angles and SHG spectrums are collected by the autocollimator and SHG-based angle sensor, respectively, for training. The mapping is learned by the trained ANN after completing the training process, which solves the inverse problem of obtaining the angle from the SHG spectrum. Then, the feasibility of the proposed method is verified in multiple-peak Maker fringe and single-peak phase-matching areas, with an overall angle measurement range exceeding 20,000 arcseconds. The predicted angles by ANN are compared with the autocollimator to evaluate the measurement performance in all the angular ranges. Particularly, a sub-arcsecond level of accuracy and resolution is achieved in the phase-matching area.
Children in the Covid-19 Pandemic: Disruptions and Opportunities
Authors: Stoecklin Daniel 
Published: July 2023

AbstractThis comment on the contributions to this volume, dedicated to children’s perspectives during the COVID-19 pandemic, identifies some transversal topics that are covered by the authors, namely distance learning, children and social bonding, social distance and construction of the self, and disruption as a chance. Reflecting children’s diverse geographies, these contributions go beyond ideological debates about childhood in the dominant representations of children as vulnerable and incompetent as they reveal both negative and positive impacts of lockdown, like exacerbated inequalities and children’s agency. The phenomenological approach, focusing on children’s understandings of the impact of the pandemic on their subjective well-being, required heightened vigilance about methodological procedures at all stages. This focus on children’s understandings is considered an epistemic opportunity that may favor more child-friendly politics.
RETRACTED ARTICLE: Research on the Application of Big Data Intelligence Technology in the Optimization of Accounts Receivable Management of E-commerce Enterprises Under the Financial Sharing Mode
Authors: Yang Xiaofeng 
Published: July 2023

AbstractAccounts receivable management has always been an important part of the financial management of the financial sharing center. However, due to manual operation, problems like long working hours, uncontrollable errors and low efficiency of invoicing still exist. To solve this problem, we study K-means clustering method to grade customer credit, and use BP model to improve the clustering algorithm. Then, we study BP model to establish enterprise risk prediction model. Finally, we use RPA to make the billing process and reconciliation as well as write-off process optimized in accounts receivable. Through the above operations, an optimized model of account receivable management of e-commerce enterprises based on big data intelligent technology has been built. According to experimental analysis, the accuracy rate of risk prediction of e-commerce enterprise A is 95.63%. After applying the optimized management model, the ratio of accounts receivable balance to current assets has decreased from 34.3% to 28.5%. Studying and constructing models can effectively optimize corporate financial management and play a positive role in the stable development of enterprises. Applying this model to practical teaching can bring new vitality to the practical teaching of vocational education and provide new teaching methods for schools. The limitations of traditional accounts receivable management limit the effectiveness of teaching for financial students. This model effectively optimizes the management mode and brings more skilled knowledge to students.
An evaluation of managed access agreements in England based on stakeholder experience
Authors: Farmer Caroline , Barnish Maxwell S. , Trigg Laura A. , Hayward Samuel , Shaw Naomi , Crathorne Louise , Strong Thomas , Groves Brad , Spoors John , Melendez Torres G. J. 
Published: July 2023

Abstract

Objectives
The objective of this research was to evaluate managed access policy in England, drawing upon the expertise of a range of stakeholders involved in its implementation.

Methods
Seven focus groups were conducted with payer and health technology assessment representatives, clinicians, and representatives from industry and patient/carer organizations within England. Transcripts were analyzed using framework analysis to identify stakeholders’ views on the successes and challenges of managed access policy.

Results
Stakeholders discussed the many aims of managed access within the National Health Service in England, and how competing aims had affected decision making. While stakeholders highlighted a number of priorities within eligibility criteria for managed access agreements (MAAs), stakeholders agreed that strict eligibility criteria would be challenging to implement due to the highly variable nature of innovative technologies and their indications. Participants highlighted challenges faced with implementing MAAs, including evidence generation, supporting patients during and after the end of MAAs, and agreeing and reinforcing contractual agreements with industry.

Conclusions
Managed access is one strategy that can be used by payers to resolve uncertainty for innovative technologies that present challenges for reimbursement and can also deliver earlier access to promising technologies for patients. However, participants cautioned that managed access is not a “silver bullet,” and there is a need for greater clarity about the aims of managed access and how these should be prioritized in decision making. Discussions between key stakeholders involved in managed access identified challenges with implementing MAAs and these experiences should be used to inform future managed access policy.
Shallow suture at ventricular septal defect may safely reduce right bundle branch block
Authors: Kim Dae Hyeon , Cho Sungkyu , Kim Woong-Han , Kwon Hye Won , Song Mi Kyoung , Kwak Jae Gun 
Published: July 2023

AbstractBackground:To avoid rhythm disturbance, sutures for ventricular septal defect closure have been traditionally placed 2∼5 mm or more away from the edge of the ventricular septal defect. However, the traditional suturing method appears to induce right bundle branch block and tricuspid valve regurgitation after ventricular septal defect closure more than our alternative technique, shallow suturing just at the edge of the ventricular septal defect (shallower bites at the postero-inferior margin). We aimed to verify our clinical experience of perimembranous ventricular septal defect repair.Methods:The alternative shallow suturing method has been applied since 2003 at our institution. We retrospectively reviewed the clinical data of 556 isolated perimembranous ventricular septal defect patients who underwent surgical closure from 2000 to 2019. We investigated the postoperative occurrence of right bundle branch block or progression of tricuspid regurgitation and analysed risk factors for right bundle branch block and tricuspid regurgitation.Results:Traditional suturing method (Group T) was used in 374 cases (66.8%), and alternative suturing method (Group A) was used in 186 cases (33.2%). The right bundle branch block occurred more frequently in Group T (39.6%) than in Group A (14.9%). In multivariable logistic regression analysis, Group T and patch material were significant risk factors for late right bundle branch block. More patients with progression of tricuspid regurgitation were found in Group T.Conclusions:Shallow suturing just at the edge of the ventricular septal defect may reduce the rate of right bundle branch block occurrence and tricuspid regurgitation progression without other complications.