bluuebunny/crossref_metadata_2025_split huggingface dataset:
What is the Optimal Number of Embryos to Transfer for Poseidon Group 1 and Group 2? A retrospective study
Authors: Liu Rang , Zhang Qiuju , Geng Lan , He Huiqing , Xu Chang , Feng Jiali , Song Miaoling , Cao Yanpei , Wang Tianren , Xia Xi 
Published: March 2023

Abstract
Background: The 2016 Patient-Oriented Strategy Encompassing IndividualizeD Oocyte Number (POSEIDON) criteria redefined the poor responders as low prognosis patients. The embryo transfer strategy for Poseidon patients remained to be addressed. This study aimed to investigate the optimized number of embryos to transfer for unexpected low-prognosis patients (Poseidon Group 1 and Group 2) with blastocyst transfer in their first frozen cycle.
Methods: A retrospective cohort study of 2970 patients who underwent frozen-thawed embryo transfer (FET) between January 2018 and December 2021. Patients from Poseidon Group 1 (N=219) and Group 2 (N=135) who underwent blastocyst transfer in their first FET cycles were included and divided into the elective single embryo transfer (eSET) group and the double embryo transfer (DET) group.
Results: For Poseidon Group 1, the live birth rateper embryo transfer of the DET group was slightly higher than the eSET group (52.17% vs 46.15%, OR 0.786, 95% CI 0.462-1.337, P = 0.374; adjusted OR (aOR) 0.622, 95% CI 0.340-1.140, P = 0.124), while a significant increase of 20.00% in the multiple birth rate was shown. For Group 2, higher live birth rates were observed in the DET group compared to the eSET group (38.46% vs 20.48%, OR 0.412, 95% CI 0.190-0.892, P = 0.024; aOR 0.358, 95% CI 0.155-0.828, P = 0.016). The difference in the multiple birth rate was 20.00% without statistical significance. Univariate and multivariate analyses revealed that age (OR 0.759, 95% CI .624-0.922, P = 0.006 and OR 0.751, 95% CI 0.605-0.932, P = 0.009) and the number of transferred embryos (OR 0.412, 95% CI 0.190-0.892, P = 0.024 and OR 0.367, 95% CI 0.161-0.840, P= 0.018) were significant variables for the live birth rate in Poseidon Group 2.
Conclusions: The findings in the present study showed that eSET was preferred in the first frozen cycle for Poseidon Group 1 to avoid unnecessary risks. Double embryo transfer strategy could be considered to improve the success rate for Poseidon Group 2 with caution. Further stratification by age is needed for a more scientific discussion about the embryo transfer strategy for Poseidon patients.
Knowledge Graph Construction based on Granulosa Cells Transcriptome from polycystic ovary syndrome with Normoandrogen and Hyperandrogen
Authors: Liu Wensu , Tang Tianyu , Feng Jianwei , Wang Chunyu , Lin Lin , Wang Shengli , Zeng Kai , Zou Renlong , Yang Zeyu , Zhao Yue 
Published: March 2023

Abstract
PCOS is a widespread disease that primarily caused in-pregnancy in pregnant-age women. Normoandrogen (NA) and Hyperandrogen (HA) PCOS are distinct subtypes of PCOS, while bio-markers and expression patterns for NA PCOS and HA PCOS have not been disclosed. We performed microarray analysis on granusola cells from NA PCOS, HA PCOS and normal tissue from 12 individuals. Afterwards, microarray data were processed and specific genes for NA PCOS and HA PCOS were identified. Further functional analysis selected IL6R and CD274 as new NA PCOS functional markers, and meanwhile selected CASR as new HA PCOS functional marker. IL6R, CD274 and CASR were afterwards experimentally validated on mRNA and protein level. Subsequent causal relationship analysis based on Apriori Rules Algorithm and co-occurrence methods identified classification markers for NA PCOS and HA PCOS. According to classification markers, downloaded transcriptome datasets were merged with our microarray data. Based on merged data, causal knowledge graph was constructed for NA PCOS or HA PCOS and female infertility on NA PCOS and HA PCOS. Gene-drug interaction analysis was then performed and drugs for HA PCOS and NA PCOS were predicted. Our work was among the first to indicate the NA PCOS and HA PCOS functional and classification markers and using markers to construct knowledge graphs and afterwards predict drugs for NA PCOS and HA PCOS based on transcriptome data. Thus, our study possessed biological and clinical value on further understanding the inner mechanism on the difference between NA PCOS and HA PCOS.
Peculiarities of Аpplication of the Institution of Exemption from Liability to Administrative Offenses in the Field of Road Safety, Recorded Automatically
Authors: Georgiievskyi Iurii , Spasenko Viktoriia , Bielikova Maryna 
Published: March 2023

The article analyzes the current state of administrative and legal regulation of the institute of exemption from liability for minor administrative offenses. The author notes that there are a number of problematic aspects of application of the Code of Ukraine on Administrative Offenses (hereinafter – CUAO) in the area under study, which is explained by the uncertainty of the legal nature of minority of an administrative offense. The lack of legislative definition of the term "minority offense", the criteria for distinguishing such an act from others, and the lack of regulation of misdemeanors which fall under the signs of minority cause problems of a law enforcement nature. The purpose of the article is to determine the shortcomings of the legal regulation of the procedure for exempting a person from responsibility for committing an administrative offense recorded in the automatic mode, due to the insignificance of the offense and formulating proposals for improving the current legislation in this area. In order to achieve the specified goal, the authors analyzes the legislation which regulates the procedure for dismissal of offenders referred to in Art. 14-2 of the CUAO in the field of road traffic safety. As a result, it has been established that despite the additional enshrinement of exceptional grounds for exemption from liability in Article 279-3 of the CUAO, the above category of persons is in a more unfavorable position than other subjects of administrative offenses. Although the general ground for exemption from administrative liability set out in Article 22 of the CUAO, which is the insignificance of an act, does not contain any restrictions on its application to automatically recorded offenses, the impossibility of its implementation is due to the specifics of the provisions of Articles 33, 268, 280 of the CUAO. The author formulates proposals for improving the legal regulation of application of Article 22 of the CUAO to offenses in this area, which will form the basis for further developments.
Inhibition of Neuromyelitis Optica Immunoglobulin G Binding to Aquaporin-4
by the Small Molecule Blocker Melanthioidine
Authors: Xu Huijing , Gong Yushuang , Jiao Yang , Guo Jian , Zhao Ling , Yang Juechen , Tong Haibin , Sun Meiyan , Li Miao 
Published: March 2023

Background:
Neuromyelitis optica (NMO) is a severe neurological demyelinating autoimmune disease
affecting the optic nerves and spinal cord. The binding of neuromyelitis optica immunoglobulin G (NMO-
IgG) and aquaporin-4 (AQP4) on the surface of astrocytes in the serum and cerebrospinal fluid is the main
pathogenesis of NMO. Currently, therapeutic strategies for NMO include a reduction of the secondary inflammation
response and the number of NMO-IgG, which can only alleviate clinical symptoms rather than fundamentally
preventing a series of pathological processes caused by NMO-IgG binding to AQP4.

Objective:
The purpose of this study was to investigate the blocking effect of melanthioidine on the binding of
NMO-IgG to AQP4 and its potential cytotoxicity.

Methods:
The current study developed a cell-based high-throughput screening approach to identify a molecular
blocker of NMO-IgG binding to AQP4 using the Chinese hamster lung fibroblast (V79) cells expressing M23-
AQP4. By screening ~400 small molecules, we identified melanthioidine with blocking effects without affecting
AQP4 expression or its water permeability.

Results:
Melanthioidine effectively blocked the binding of NMO-IgG to AQP4 in immunofluorescence assays
and reduced complement-dependent cytotoxicity against both NMO-IgG/complement-treated Fischer rat thyroid-
AQP4 cells and primary astrocytes. The docking computations identified the putative sites of blocker binding
at the extracellular surface of AQP4.

Conclusion:
This study serves as proof of a potential NMO therapy by using a small-molecule blocker to target
NMO pathogenesis.
Clinical Usefulness of Ultrasound Elastography in Colonic Diseases: A Narrative
Review
Authors: Qiu Zhongxian , Zheng Ruoting , Lin Jiawei , Li Jiafan , Zhai Yuxia 
Published: March 2023

Abstract:
Ultrasound elastography is an innovation of ultrasound technology that has developed since the 1990s. It has been successfully applied for many organs, such as the thyroid, breast, liver, prostate, and muscle systems, providing qualitative and quantitative information about tissue stiffness for clinical diagnoses. For colorectal tumors, ultrasound elastography can distinguish colon adenoma from colon adenocarcinoma and predict the chemotherapeutic effects of colon cancer by monitoring the stiffness changes of cancer tissue. In Crohn’s disease, ultrasound elastography helps assess the stages of the course and guides further treatment strategies. Compared with colonoscopy, ultrasound elastography frees patients from the fears of uncomfortable procedures and enables operators to comprehensively observe the bowel wall and the surrounding structures. In this review, we introduced the principles and the pathological basis of ultrasound elastography and compared the diagnostic efficacies of colonoscopy with colonic ultrasound elastography. Meanwhile, we summarized the ultrasonography of colonic diseases and reviewed the clinical usefulness of ultrasound elastography in colonic diseases.
Current Landscape on Development of Phenylalanine and Toxicity of its
Metabolites - A Review
Authors: Bose Samrat , Mandal Shirsendu , Khan Rajesh , Maji Himangshu Sekhar , Ashique Sumel 
Published: March 2023

Abstract:
Phenylalanine, an essential amino acid, is the "building block" of protein. It has a tremendous
role in different aspects of metabolic events. The tyrosine pathway is the prime one and is typically used
to degrade dietary phenylalanine. Phenylalanine exceeds its limit in bodily fluids and the brain when the
enzyme, phenylalanine decarboxylase, phenylalanine transaminase, phenylalanine hydroxylase (PAH) or
its cofactor tetrahydrobiopterin (BH4) is deficient causes phenylketonuria, schizophrenia, attentiondeficit/
hyperactivity disorder and another neuronal effect. Tyrosine, an amino acid necessary for synthesizing
the pigments in melanin, is produced by its primary metabolic pathway. Deficiency/abnormality in
metabolic enzymes responsible for the catabolism pathway of Phenylalanine causes an accumulation of
the active intermediate metabolite, resulting in several abnormalities, such as developmental delay, tyrosinemias,
alkaptonuria, albinism, hypotension and several other undesirable conditions. Dietary restriction
of the amino acid(s) can be a therapeutic approach to avoid such undesirable conditions when
the level of metabolic enzyme is unpredictable. After properly identifying the enzymatic level, specific
pathophysiological conditions can be managed more efficiently.
The Zebrafish Model as a New Discovery Path for Medicinal Plants in the Treatment of Parkinson’s Disease
Authors: Chahardehi Amir Modarresi , Hosseini Yasaman , Mahdavi Seyed Mohammad , Naseh Iman 
Published: March 2023

Abstract:
Parkinson's disease (PD) is one of the most frequent degenerative central nervous system
disorders affecting older adults. Dopaminergic neuron failure in the substantia nigra is a pathological
sign connected with the motor shortfall of PD. Due to their low teratogenic and adverse effect potential,
medicinal herbs have emerged as a promising therapy option for preventing and curing PD and
other neurodegenerative disorders. However, the mechanism through which natural compounds provide
neuroprotection against PD remains unknown. While testing compounds in vertebrates such as
mice is prohibitively expensive and time-consuming, zebrafish (Danio rerio) may offer an appealing
alternative because they are vertebrates and share many of the same characteristics as humans.
Zebrafish are commonly used as animal models for studying many human diseases, and their molecular
history and bioimaging properties are appropriate for the study of PD. However, a literature review
indicated that only six plants, including Alpinia oxyhylla, Bacopa monnieri, Canavalia gladiate, Centella
asiatica, Paeonia suffruticosa, and Stachytarpheta indica had been investigated as potential PD
treatments using the zebrafish model. Only C. asiatica and B. monnieri were found to have potential
anti-PD activity. In addition to reviewing the current state of research in this field, these plants' putative
mechanisms of action against PD are explored, and accessible assays for investigation are made.
PERENCANAAN KAWASAN HUNIAN KOMUNAL SEMENTARA DENGAN KONSEP MULTI-FUNGSI DALAM PERSPEKTIF PENGEMBANGAN WILAYAH KOTA PALU
Authors: Anisa  , Saputra Ashar , Satyarno Iman 
Published: March 2023

Gempa bumi 2018 di Kota Palu menyebabkan kerusakan fasilitas umum dan aset masyarakat, hal ini mengharuskan para korban mengungsi ke hunian sementara hingga rumah mereka selesai direkonstruksi atau memperoleh hunian baru. Problematika masyarakat menolak relokasi, masyarakat tidak nyaman tinggal di hunian sementara, hingga pembangunan fasilitas hunian sementara yang meningkatkan biaya pembangunan. Hal ini memecah fokus rehabilitasi dan rekonstruksi. Berdasarkan fenomena tersebut dapat disimpulkan bahwa dalam situasi darurat regulasi tidak lagi diprioritaskan, sehingga fasilitas penting seperti hunian sementara perlu dipersiapkan secara matang. Penelitian ini bertujuan merencanakan sistem fasilitas hunian sementara dengan konsep multi-fungsi. Pengumpulan data dilakukan dengan survey instansional, pengisian kuesioner, dan mengkaji pustaka. Penelitian ini dilakukan secara kuantitatif dengan menggunakan metode Analisis Hirarki Proses (AHP), dimana peneliti menggunakan kriteria dari 4 Stakeholder untuk menentukan lokasi alternatif yang cocok dikembangkan menjadi kawasan hunian komunal sementara. Penelitian ini juga mengkolaborasikan analisis klasifikasi tingkat bahaya dan klasifikasi kepadatan penduduk. Hasil dari penelitian ini menunjukkan bahwa kawasan hunian komunal sementara terdiri atas tiga hirarki yaitu Kecamatan Mantikulore sebagai Pusat hunian komunal sementara. Kecamatan Palu selatan, Kecamatan Tatanga dan Kecamatan Ulujadi, Kecamatan Palu Utara, dan Kecamatan Tawaeli akan menjadi Sub-pusat pelayanan hunian komunal sementara. adapun Kecamatan Palu Barat dan Kecamatan Palu Timur menjadi kawasan terlayani.
Kata Kunci : huntara, hirarki pelayanan, multifungsi, Kota Palu
The Insulin-Only Bionic Pancreas Improves Glycemic Control in Non-Hispanic White and Minority Adults and Children with Type 1 Diabetes
Authors: Castellanos Luz E. , Russell Steven J. , Damiano Edward R. , Beck Roy W. , Shah Viral N. , Bailey Ryan , Calhoun Peter , Bird Keisha , Mauras Nelly , Group the Bionic Pancreas Research 
Published: March 2023

<p> </p>
<p><strong>Objective: </strong>We evaluated the performance of the iLet® bionic pancreas (BP) in non-Hispanic Whites (‘Whites’) and in Blacks, Hispanics, and others (‘Minorities’).</p>
<p><strong>Research Design and Methods:</strong> A multicenter, randomized controlled trial evaluated glycemic management with the BP versus standard-of-care (SC) in 161 adult and 165 pediatric participants with type 1 diabetes over 13 weeks. </p>
<p><strong>Results:</strong> In Whites (N=240), the mean baseline-adjusted difference in 13-week HbA1c between the BP and SC groups was -0.45% (95% CI -0.61% to -0.29% [-4.9, -6.6 to -3.1 mmol/mol]; P<0.001), while this difference among Minorities (N=84) was -0.53% (-0.83% to -0.24% [-6.0, -9.2 to -2.8 mmol/mol]; P<0.001). In Whites, the mean baseline-adjusted difference in TIR between the BP and SC groups was 10% (7 to 12%; P<0.001), while in Minorities it was 14% (10 to 18%; P<0.001). </p>
<p><strong>Conclusions:</strong> The BP improves glycemic control in both Whites and Minorities and offers promise in decreasing health care disparities. </p>
Science Teachers Competencies and Problem in Implementing 2013 Curriculum at Primary and Secondary School in Bali
Authors: Paraniti Anak Agung Inten , Suma Ketut 
Published: March 2023

Teachers are the key to success in implementing the curriculum; however, many studies report problems implementing the K-13 curriculum, including teachers' competencies. There has been no thorough report on teacher competence related to issues in the implementation. This study examines science teacher competencies in three main aspects, namely skill to design, manage and implement learning in the K-13 curriculum. Mix method was used in this study with a concurrent mixed method design. First, the survey involved 67 respondents who filled out a science teacher competencies questionnaire based on the process standards of the K-13 curriculum. Meanwhile, the qualitative part was conducted by conducting structured interviews with 12 science teachers about challenges and problems related to three main aspects of science teacher competencies in implementing the k-13 curriculum. Quantitative data in percentages is collected, while qualitative data analysis is done through data reduction, presentation, and verification. The competence of teachers in learning design must be further improved in creativity and goal setting to match the allocation of time, activities, dan learning assessments in the process of making learning plans. Science teachers' competence should be improved in arranging the seats according to the goal and characteristics of education. The competence of implementation must be further improved in two indicators: the creativity of learning implementation, which is in line with the teacher's understanding of various innovative learning models, and learning implementation, in line with the lesson plan.
Efficiency of diagnosing the condition of rolling bearings in real time
Authors: Barashkova Tatiana , Shirokova Veroonika 
Published: March 2023

One of the control elements of a complex technological system is an information-and-measuring network, a significant part of which is represented by measuring transducers and sensors. Due to the design features, the sensors turn out to be unreliable elements of information-and-measuring systems. Distortions in sensor readings affect the quality of controlling the technical system. It is necessary to account for these distortions when assessing the efficiency of the diagnostics of mechanical components. This fact is especially significant when assessing the efficiency of diagnosing the condition of rolling bearings in real time.
It is proved that the efficiency of diagnosing the machine parts largely depends on the dynamics of the information-and-measuring system and the prompt detection of changes in the operation of this system. As a result of the experiments, the primary task to diagnose the machine parts was solved on the example of faulty rolling bearings, and the influence of the computing power of the equipment used, the sensitivity of the acceleration sensors, and practical feasibility of the network algorithms for automatic data processing in real time were assessed.
Methods of calculating total hemocyte count in Australian red-claw crayfish
Authors: Skafar' Denis Nikolaevich , Shumeyko Dmitriy Valentinovich 
Published: March 2023

The optimal formula for calculating the total number of hemocytes (HTN) of the Australian red-claw crayfish (Cherax quadricarinatus) has been established, which allows optimizing the time of counting HTN without re-ducing accuracy. Methodical recommendations on preparing a hemolymph sample, HTN calculation and differentiated calculation of hemocytes are given. It was found out that the differences in HTN are minimal when using different formulas, which is con-firmed by the analysis of variance F = 0.011, p = 0.998, the average values of HTN range from 2 384 to 2 427 cell/µl. Conducting multiple comparisons also demonstrated that the differences between the formulas are minimal: the significance levels between the formulas were p = 0.99. The optimal formula for the Australian red-claw crayfish is: HTN in 1 µl = N • 10, where N is the total number of hemocytes in 25 large squares of Gorjaev’s chamber. The counting chamber should be clean, since when even small dust particles are found on the grid, hemocyte clusters can form and impede counting, or the result of the calculation will be incorrect, because the distribution
of hemocytes on the grid will not be uniform. After filling the chamber with hemolymph, it is recommended to wait
1-1.5 minutes so that the movement of the hemolymph stops and the hemocytes stop moving. It is recommended to use an anticoagulant, which prevents the rapid decay of cells and promotes their uniform distribution on the grid of the chamber without developing clots (clusters) that affect the accuracy of counting. Working with the whole hemolymph implies the speed and accuracy of the researcher's actions, since it quickly coagulates and forms clusters of hemocytes. In order to avoid confusion when counting cells located on the border between squares, Egorov’s rule is applied. The optimal increase for counting is a 400-fold increase.
Antibacterial potential of celery leaf extract toothpaste on the growth of Streptococcus mutans ATCC 25175
Authors: Hayati Maulida , Mailiza Fitria , Savitri Shifa Khumaira 
Published: March 2023

ABSTRACTIntroduction: Celery (Apium graveolens L.) is a member of the Apiaceae family that can grow everywhere. Celery contains flavonoids, saponins, tannins, essential oils, apigenin, choline, vitamins A, B, C, asparagine bitter substances. Celery contains flavonoids, saponins, and tannins which are antibacterial compounds. Streptococcus mutans is a Gram-positive bacterium creating an acidic atmosphere in the oral cavity, which causes insufficient saliva to prevent enamel demineralisation which can lead to dental caries. The purpose of this study was to analyze the inhibition effect of celery leaf extract (Apium graveolens L.) on the growth of Streptococcus mutans. Methods: This type of experimental laboratory research uses the Post Test Only Control Group Design. The samples were Apium graveolens L. and Streptococcus mutans ATCC 25175. The number of treatment groups was 24 with concentrations of 20, 40, 60, and 80%. Toothpaste containing antibacterial ingredients was used as positive control and for negative control, a basic toothpaste formula without antibacterial ingredients, namely carboxymethyl cellulose (CMC), xylitol, calcium carbonate (CaCO3), sorbitol, sodium lauryl sulphate (SLS), natrium benzoate. Data analysis using one-way ANOVA test with normality test using Shapiro-Wilk and homogeneity test using Levene test. The results at a concentration of 20, 40, 60 and 80% with a positive control there was no significant difference because the sig value was >0.05. Results: The results showed that Celery containing toothpaste inhibits Streptococcus mutans growth (p value <0.05) that there is a significant inhibition effect of Apium graveolens L. extract toothpaste on the growth of Streptococcus mutans. Conclusion: There is an inhibition effect of celery leaf (Apium graveolens L.) extract toothpaste on the growth of Streptococcus mutans.Keywords: streptococcus mutans ATCC 25175, inhibition, toothpaste, celery, apium graveolens L