bluuebunny/crossref_metadata_2025_split huggingface dataset:
Storm: Incorporating transient stochastic dynamics to infer the RNA velocity with metabolic labeling information
Authors: Peng Qiangwei , Qiu Xiaojie , Li Tiejun 
Published: June 2023

AbstractThe time-resolved scRNA-seq (tscRNA-seq) provides the possibility to infer physically meaningful kinetic parameters, e.g., the transcription, splicing or RNA degradation rate constants with correct magnitudes, and RNA velocities by incorporating temporal information. Previous approaches utilizing the deterministic dynamics and steady-state assumption on gene expression states are insufficient to achieve favorable results for the data involving transient process. We present a dynamical approach, Storm (Stochastic models of RNA metabolic-labeling), to overcome these limitations by solving stochastic differential equations of gene expression dynamics. The derivation reveals that the new mRNA sequencing data obeys different types of cell-specific Poisson distributions when jointly considering both biological and cell-specific technical noise. Storm deals with measured counts data directly and extends the RNA velocity methodology based on metabolic labeling scRNA-seq data to transient stochastic systems. Furthermore, we relax the constant parameter assumption over genes/cells to obtain gene-cell-specific transcription/splicing rates and gene-specific degradation rates, thus revealing time-dependent and cell-state specific transcriptional regulations. Storm will facilitate the study of the statistical properties of tscRNA-seq data, eventually advancing our understanding of the dynamic transcription regulation during development and disease.
Uncovering novel endolysins against methicillin-resistantStaphylococcus aureususing microbial single-cell genome sequencing
Authors: Yoda Takuya , Matsuhashi Ayumi , Matsushita Ai , Shibagaki Shohei , Sasakura Yukie , Aoki Kazuteru , Hosokawa Masahito , Tsuda Soichiro 
Published: June 2023

AbstractEndolysins, peptidoglycan hydrolases derived from bacteriophages (phages), are being developed as a promising alternative to conventional antibiotics. To obtain highly active endolysins, a diverse library of endolysins is vital. We here propose microbial single-cell genome sequencing as an efficient tool to discover dozens of previously unknown endolysins, owing to its culture-independent sequencing method. As a proof-of-concept, we analyzed and recovered endolysin genes within prophage regions ofStaphylococcussingle-amplified genomes (SAGs) in human skin microbiome samples. We constructed a library of chimeric endolysins by shuffling domains of the natural endolysins and performed high-throughput screening againstStaphylococcus aureus. One of the lead endolysins, bbst1027, exhibited desirable antimicrobial properties such as rapid bactericidal activity, no detectable resistance development, andin vivoefficacy. We foresee that this endolysin discovery pipeline is in principle applicable to any bacterial target, and boost the development of novel antimicrobial agents.
Purine nucleotide limitation undermines antibiotic action in clinicalEscherichia coli
Authors: Lubrano Paul , Schramm Thorben , Lorenz Elisabeth , Alvarado Alejandra , Eigenmann Seraina Carmen , Stadelmann Amelie , Thavapalan Sevvalli , Waffenschmidt Nils , Glatter Timo , Peter Silke , Drescher Knut , Link Hannes 
Published: June 2023

AbstractMetabolic variation across pathogenic bacterial strains can impact their susceptibility to antibiotics1–4and promote evolution of antimicrobial resistance (AMR)5,6. However, little is known about which metabolic pathways contribute to AMR, and the underlying mechanisms. Here, we measured antibiotic resistance of 15,120Escherichia colimutants, each with a single amino acid change in one of 346 essential proteins. Most of the mutant strains that showed resistance to either of the two tested antibiotics carried mutations in metabolic genes. Resistance mutations against a β-lactam antibiotic (carbenicillin) were associated with purine nucleotide biosynthesis and limited the supply of ATP. We show that ATP limitation confers both resistance and tolerance against β-lactam antibiotics by upregulating the purine nucleoside transporter PunC. These results are clinically relevant, because anE. colistrain isolated from a clinical specimen had a purine nucleotide limitation, which reduced its susceptibility to antibiotics.
Flagellar switch inverted repeat impacts flagellar invertibility and variesClostridioides difficileRT027/MLST1 virulence
Authors: Nhu Nguyen T. Q. , Lin Huaiying , Pigli Ying , Sia Jonathan K. , Kuhn Pola , Snitkin Evan S. , Young Vincent , Kamboj Mini , Pamer Eric G. , Rice Phoebe A. , Shen Aimee , Dong Qiwen 
Published: June 2023

SUMMARYClostridioides difficileRT027 strains cause infections that vary in severity from asymptomatic to lethal, but the molecular basis for this variability is poorly understood. Through comparative analyses of RT027 clinical isolates, we determined that isolates that exhibit greater variability in their flagellar gene expression exhibit greater virulencein vivo.C. difficileflagellar genes are phase-variably expressed due to the site-specific inversion of theflgB5’UTR region, which reversibly generates ON vs. OFF orientations for the flagellar switch. We found that longer inverted repeat (IR) sequences in this switch region correlate with greater disease severity, with RT027 strains carrying 6A/6T IR sequences exhibiting greater phenotypic heterogeneity in flagellar gene expression (60%-75% ON) and causing more severe disease than those with shorter IRs (> 99% ON or OFF). Taken together, our results reveal that phenotypic heterogeneity in flagellar gene expression may contribute to the variable disease severity observed inC. difficilepatients.
Auditors' sensemaking of other comprehensive income through metaphors
Authors: Durocher Sylvain , Picard Claire-France , Dugal Léa 
Published: June 2023

PurposeThis paper aims to examine how auditors make sense of the ill-theorized and contentious notion of other comprehensive income (OCI), specifically by uncovering their use of metaphors to make OCI plausible and intelligible.Design/methodology/approachThis interpretative paper draws on a collection of 21 interviews with experienced auditors. The analysis first uncovers metaphors that naturally surface within the talk and sensemaking of auditors about OCI (elicited metaphors). The authors then encapsulate these elicited metaphors into second-order constructs (projected metaphors) to synthesize and further explain auditors’ practical sensemaking.FindingsAuditors conceive OCI as a “safety” that ensures the well-functioning of fair value accounting, metaphorically qualifying this notion as a “necessary evil”, a “passage obligé”, and a “parking lot” resolving fair value-related issues and aberrations. Auditors also metaphorize OCI as a “purifier” that allows “polluted”, “noisy”, and “unloved” items to be “parked” outside net income.Practical implicationsThe study’s findings further the understanding of auditors’ tendency to remain uncritical throughout their sensemaking process. Making sense of professional standards of practice through metaphors indubitably involves shadowing and silencing other worldviews.Originality/valueThis paper extends knowledge of auditors’ sensemaking, specifically showing how auditors easily make sense of complex notions even in the absence of conceptual grounds. This study also highlights that metaphors are a powerful sensemaking device that auditors mobilize to render complex notions intelligible and mitigate IFRS inconsistencies.
Impact of e-servicescape on hotel booking intention: examining the moderating role of COVID-19
Authors: Srivastava Praveen , Srivastava Shelly , Mishra Niraj 
Published: June 2023

Purpose
The paper aims to illuminate the relationship between attitude toward the hotel’s website (e-servicescape) and purchase intention. It proposes modeling the dimension of the hotel’s website, its impact on building attitude toward the website and the relations with purchase intention. Further, the role of social risk arising from COVID-19 on the relationship between attitude and purchase intention is explored.

Design/methodology/approach
The authors conducted empirical research using an online questionnaire based on a comprehensive evaluation of prior literature. The online questionnaire has 15 questions, and data is gathered from guests who have visited the hotel’s website in the preceding year. SmartPLS was used to analyze the data. Attitude toward the website has been identified as a higher-order factor, and a two-stage embedding technique was adopted for analysis.

Findings
The paper gives empirical evidence about how different dimensions of a hotel’s website influence one’s attitude. The finding indicates that the ambience factor, i.e. how effectively a website provides a pleasant and interesting atmosphere for online visitors, is the most critical component for attitude development. Additionally, the finding reveals the negative moderating effect of social risk on the relationship between attitude and purchase intention.

Research limitations/implications
Since demographic factors play a significant role in the social-risk component, the study findings may lack generalizability. As a result, scholars are urged to do more study on the offered ideas.

Practical implications
The paper includes implications for the development of a better e-servicescape, which should incorporate the dimensions discussed in the study to entice the customers for hotel booking and can remove the fear of social risk.

Originality/value
This paper addresses an established need by examining how social risk affects the relationship between attitude toward a website and purchase intention.
Does corporate governance matter in the cleanup of reported oil spills? Evidence from Nigeria
Authors: Abdul-Baki Zayyad , Haniffa Roszaini , Diab Ahmed 
Published: June 2023

Purpose
This study aims to examine whether corporate governance mechanisms – board size, board independence and CEO duality – influence the actions of oil companies operating in Nigeria to clean up oil spills from their facilities.

Design/methodology/approach
Both binary logistic regression (linear) and random-effects logistic regression models were used to test three hypotheses using a unique data set of 1,262 oil spill events involving 24 oil companies from 2017 to 2019.

Findings
The study found that board size and board independence are positively related to oil spill cleanup.

Practical implications
Private oil companies in Nigeria should encourage larger and more independent boards in their corporate governance (CG) structures, as these boards may be more effective in serving the interests of stakeholders by bringing diverse knowledge and experience to the boards. Similarly, regulators should extend the enforcement of CG codes to private firms.

Originality/value
To the best of the authors’ knowledge, this is the first study that investigates the influence of CG attributes on oil spill cleanup.
Can tourism enhance inclusitivity for indigenous peoples? Cham perspectives on tourism benefit sharing at living heritage sites in Vietnam
Authors: Quang Tuyen Dai , Dang Vang Quang , Alang Tho , Nguyen Hoang Van 
Published: June 2023

PurposeThrough a case study of the Po Klaong Girai temple in Vietnam, this paper explores how indigenous community perceive tourism benefit sharing (TBS) associated with their cultural tourism at sacred living-heritage sites and how this TBS enhances the equality and inclusion for indigenous community in the context of tourism in Vietnam.Design/methodology/approachThis study employed ethnographic fieldwork and semi-structured interviews with 35 indigenous Cham priests directly associated with managing and preserving the Po Klaong Girai temple.FindingsThis research found that Cham community perceive inequality and exclusivity on tourism benefit sharing at this religious site. While Cham Ahier priests face economic barriers in providing these services to the community, annual tourism revenue is allocated to local government budgets. Such economic pressure forces the priests to seek alternative economic avenues to support their families at the expense of their traditional commitments to communal, cultural and religious activities, significantly impacting sustainable heritage conservation. This has led to strained relations between the local community and local authorities.Practical implicationsThis research provides evidence to improve living heritage management practices by proposing tourism development policies for equality and inclusion among stakeholders, especially minorities and disadvantaged groups. This can be an experiential and necessary lesson for “dealing” with sustainable heritage management in heritage living sites in other ethnic minority areas in Vietnam and globally.Originality/valueThe findings from this study address the knowledge gap on equitable revenue sharing in heritage tourism, where financial benefits from the commodification of minority cultures should be used to support local communities and the custodians of indigenous heritage.
Alternative ecotourism perspectives within the protected conservation sites and farming communities amid environmental degradation and climate change-bound rural exercises
Authors: Ramaano Azwindini Isaac 
Published: June 2023

PurposeThis study aims to examine the latent capability of alternative-responsible tourism and ecotourism management in sustainable tourism and rural communities' livelihoods in Musina Municipality in Limpopo Province, South Africa, and benchmark the capability with other African countries amid climate change and environmental degradation dilemmas. Hence, the aforementioned objective coordinates with various agrarian locations elsewhere abroad.Design/methodology/approachLiterature reviews, document reviews, interviews, and focus group discussions were employed to gather data, which was enriched by field observation views. To this end, potential climate change-linked environmental degradation, rural tourism, agricultural, and environmental management issues came to the fore.FindingsEnvironmental degradation poses a possible threat to natural resource management, as evidenced by the rural development, ecotourism and agricultural activities within the study region. Thereupon, environmental degradation supplements the probability of adverse effects from climate change and precarious incomes. According to the research, responsible tourism and ecotourism are vital to promoting sustainability in rural localities.Originality/valueThe plenteous biodiversity of the municipality offers a better scenario for sustainable ecotourism in tandem with agritourism efforts to address the identified ongoing and liability issues. These issues have therefore symbolized the need for an adequate and allied tourism strategy to boost the local people in Musina Municipality and, conceivably, throughout the continent.
Retaining U.S. stem women faculty during the COVID-19 pandemic: the important role of family-supportive supervisor behaviors to lower work-to-life conflict
Authors: Lawson Katie M. , Lee Soomi , Smith Claire , Thiem Kelsey C. 
Published: June 2023

Purpose
The COVID-19 pandemic may negatively impact the careers of U.S. women faculty in computer science (CS) – a field with few women and high attrition rates among women – due to difficulties balancing increased work and family demands (author citation). Thus, it is important to understand whether supervisors may help to decrease this work-to-life conflict and increase the odds of retaining women faculty. This study examined whether family-supportive supervisor behaviors (FSSB) were associated with better retention-related job outcomes (e.g., lower turnover intentions) among women faculty in CS, and whether this association was mediated by lower work-to-life conflict.

Design/methodology/approach
A total of 379 CS faculty across the U.S. (54% women, 52% with children at home) were surveyed during the COVID-19 pandemic (Fall 2020-Spring 2021).

Findings
Compared to men, women CS faculty reported significantly higher work-to-life conflict, and that higher work-to-life conflict predicted poorer retention-related work outcomes for all faculty. However, for women only, FSSB predicted lower levels of work-to-life conflict, and in turn, better retention-related work outcomes.

Practical implications
Results suggest that emotionally-supportive supervisor behaviors may lower work-to-life conflict during the pandemic, which has important implications for retention-related work outcomes among women faculty in CS.

Originality/value
Research has yet to disentangle how managers can help to retain women CS faculty in light of the increasing and changing work and home demands due to the pandemic. The present study focused on whether FSSB – particularly emotional support – may benefit women CS faculty.
Metaverse meets digital entrepreneurship: a practitioner-based qualitative synthesis
Authors: Calandra Davide , Oppioli Michele , Sadraei Razieh , Jafari-Sadeghi Vahid , Biancone Paolo Pietro 
Published: June 2023

PurposeConsidering the pervasiveness of technology, this article offers an understanding of how the metaverse can impact digital entrepreneurship. The objective will be to gather professional evidence on how the revival of this new technology can bring entrepreneurial development.Design/methodology/approachThrough a qualitative study approach based on applying the metaverse in digital business contexts and analysing 533 practitioner sources from the Nexis Uni database, it will be possible to identify the concepts and application techniques of this emerging technology. The research adopts a qualitative methodology based on a mixed thematic and content review using tools such as ATLAS.ti and Leximancer.FindingsThis study finds three relevant macro-topics for metaverse and digital entrepreneurship (technology, immersive and design) and eight concepts (private solutions, digital twins, gamification, public solutions, new business worlds, co-design, collaborative spaces and stakeholders' participation). The uncovered elements demonstrate professionals' interest in a new mode of digital entrepreneurship using the metaverse. This interest highlights the commitment of companies and entrepreneurs toward discovering new services delivered in virtual and parallel worlds that find the creation of digital twins as their essence. Therefore, the study explores ongoing relationships for developing increasingly technically complex metaverse platforms and customer service offerings.Research limitations/implicationsThe study has some limitations as the selection of the database and the way the cases are focused on, which may be a stimulus for future studies. The analysis has innumerable theoretical and practical implications. In the first case, this research will shed light on an empirical case concerning the conceptual difference between innovation or greater transformation of business models through the metaverse. This work will directly contribute to the global discussion by identifying a model for applying emerging technology to digital entrepreneurs. Finally, from a practical point of view, the authors will provide new insights to digital entrepreneurs by showing them applications, best practices, and platforms they can use for their businesses.Practical implicationsOn a practical level, the authors show practical opportunities coming from the metaverse for digital entrepreneurs. This study may inspire CEOs, managers, and future entrepreneurs to use the metaverse to expand their businesses by diversifying their services into numerous sectors.Originality/valueTo the best of the authors' knowledge, this study represents one of the first efforts to study the metaverse by framing it from theoretical and practical perspectives of digital entrepreneurship. Additionally, future research implications may guide researchers in this brilliant research field.
Enhancing the website usage using process mining
Authors: Choudhary Chetna , Mehrotra Deepti , Shrivastava Avinash K. 
Published: June 2023

PurposeAs the number of web applications is increasing day by day web mining acts as an important tool to extract useful information from weblogs and analyse them according to the attributes and predict the usage of a website. The main aim of this paper is to inspect how process mining can be used to predict the web usability of hotel booking sites based on the number of users on each page, and the time of stay of each user. Through this paper, the authors analyse the web usability of a website through process mining by finding the web usability metrics. This work proposes an approach to finding the usage of a website using the attributes available in the weblog which predicts the actual footfall on a website.Design/methodology/approachPROM (Process Mining tool) is used for the analysis of the event log of a hotel booking site. In this work, authors have used a case study to apply the PROM (process mining tool) to pre-process the event log dataset for analysis to discover better-structured process maps than without pre-processing.FindingsThis article first provided an overview of process mining, then focused on web mining and later discussed process mining techniques. It also described different target languages: system nets (i.e. Petri nets with an initial and a final state), inductive miner and heuristic miner, graphs showing the change in behaviour of the dataset and predicting the outcome, that is the webpage having the maximum number of hits.Originality/valueIn this work, a case study has been used to apply the PROM (process mining tool) to pre-process the event log dataset for analysis to discover better-structured process maps than without pre-processing.
Sustainability-oriented commerce innovation: How does it influence consumer satisfaction?
Authors: Gil-Saura Irene , Ruiz-Molina Maria-Eugenia , Marín-García Antonio , Michel Géraldine 
Published: June 2023

PurposeInnovation and sustainability are two key factors for retailers seeking a competitive advantage. However, the way in which the joint effect of both of these variables impacts consumer satisfaction is still unknown. To address this gap, based on the concept of sustainability-oriented service innovation (SOSI), the authors introduce a new construct named sustainability-oriented commerce innovation (SOCI) in the context of the retail sector.Design/methodology/approachThe relationships between the variables defined in this research were examined using a structural equations model for 510 customers of grocery retail establishments.FindingsThe authors find support for a direct positive impact of SOCI on customer satisfaction and an indirect impact through store equity. These chained effects are modified according to the client participation in the development of sustainable and innovative initiatives.Originality/valueThis research analyses the joint effect of innovation and sustainability in the retail context by introducing a new concept – SOCI – and a scale for its measurement whose psychometric properties are validated.