bluuebunny/crossref_metadata_2025_split huggingface dataset:
Working together to increase Australian children’s liking of vegetables: a position statement by the Vegetable Intake Strategic Alliance (VISA)
Authors: Cox David Nicholas , Campbell Karen J , Cobiac Lynne , Gardner Claire , Hancock Lucinda , Hendrie Gilly A , Kelaart Amber , Lausen Michelle , Poelman Astrid AAM , Sambell Ros , Tikellis Kim M , Wiggins Bonnie 
Published: July 2023

AbstractChildren need to be repeatedly and consistently exposed to a variety of vegetables from an early age to achieve an increase in vegetable intake. A focus on enjoyment and learning to like eating vegetables at an early age is critical to forming favourable lifelong eating habits. Coordinated work is needed to ensure vegetables are available and promoted in a range of settings, using evidence-based initiatives, to create an environment that will support children’s acceptance of vegetables. This will help to facilitate increased intake and ultimately realise the associated health benefits. The challenges and evidence base for a new approach are described.
Implementing front-of-pack nutrition warning labels in Mexico: important lessons for low- and middle-income countries
Authors: Crosbie Eric , Otero Alvarez Margarita Gabriela , Cao Michelle , Vejar Renteria Lesly Samara , Rodriguez Estefania , Larrañaga Flota Ana , Carriedo Angela 
Published: July 2023

AbstractObjective:To analyse the implementation of front-of-pack nutrition labelling (FOPNL) in Mexico.Design:Review of publicly accessible documents, including legislative websites, news sources, and government, intergovernmental, and advocacy reports. Usage of the policy cycle model to analyse the implementation and evaluation stages of Mexico’s General Health Law, amended with FOPNL (2019–2022).Results:In October 2019, the government published a draft modification of the Norma Oficial Mexicana (Official Mexican Standard) to regulate and enforce a new FOPNL warning label system. A 60-d public consultation period followed (October–December 2019), and the regulation was published in March 2020 and implementation began in October 2020. An analysis of nine key provisions of the Standard revealed that the food and beverage industry and its allies weakened some original provisions including health claims, warnings for added sweeteners and display areas. On the other hand, local and international public health groups maintained key regulations including the ban on cartoon character advertisements, standardised portions and nutrient criteria following international best practices. Early implementation appears to have high compliance and helped contribute to reformulating unhealthy products. Continued barriers to implementation include industry efforts to create double fronts and market their cartoon characters on social media and through digitalised marketing.Conclusion:Early success in implementing the new FOPNL system in Mexico was the result of an inclusive and participatory regulatory process dedicated to maintaining public health advances, local and international health advocacy support, and continued monitoring. Other countries proposing and enacting FOPNL should learn from the Mexican experience to maintain scientifically proven best practices, counter industry barriers and minimise delays in implementation.
Outer Rainband Formation on Land Ahead of Typhoon Hato (2017)
Authors: Ruan Tianming , Chen Guixing , Bai Lanqiang 
Published: July 2023

AbstractAn outer rainband develops rapidly on land of South China from afternoon to evening of 22 August 2017 when Typhoon Hato is approaching, leading to an early arrival of convective storms prior to typhoon's landfall. The convective cells initiate on the eastern coasts and move to the western inland region where they grow upscale into a fully fledged outer rainband. Convective‐permitting simulations suggest that the new‐born convective cells on the eastern coasts are associated with the increasing convective instability and low‐level lifting by the land‐sea differences of non‐gradient winds. Such differences are established by the horizontal and vertical components of thermal contrasts. In the following stage of convection organization, the convectively induced cold pools in turn strengthen the updrafts at their leading edges, which help to organize the convective cells on rainband as a fast‐moving squall line in the western region. Such storm‐feedback processes of rainband convection and cold pools strongly respond to the changing convective instability and mid‐level dry conditions on land. These regional environmental conditions are closely associated with the large‐scale subsidence in the outer region of typhoon. The results highlight that the new outer rainband can form rapidly on land due to surface heating, and the storm‐feedback processes in response to the changing environmental conditions when a tropical cyclone is approaching land.
Porous Melt Flow in Continental Crust—A Numerical Modeling Study
Authors: Maierová P. , Hasalová P. , Schulmann K. , Štípská P. , Souček O. 
Published: July 2023

AbstractIn continental crust, rapid melt flow through macroscopic conduits is usually envisaged as the most efficient form of melt transport. In contrast, there is growing evidence that in hot continental crust, grain‐scale to meso‐scale porous melt flow may operate over long distances and over millions of years. Here, we investigate the dynamics of such porous melt flow by means of two‐dimensional thermo‐mechanical numerical models using the code ASPECT. Our models are crustal‐scale and describe the network of pores through which the melt flows by permeability that depends on the spacing of the pores. Our results suggest that assuming realistic material properties, melt can slowly migrate in the hot and thick continental crust through pores with a characteristic spacing of 1 mm or larger. Despite its low velocity (millimeters to centimeters per year), over millions of years, such flow can create large partially molten zones in the middle‐lower crust and significantly affect its thermal state, deformation, and composition. We examined the role of the permeability, melt and solid viscosities, the slope of the melting curve and temperature conditions. We obtained contrasting styles of melt distribution, melt flow, and solid deformation, which can be categorized as melt‐enhanced convection, growth of partially molten diapirs and melt percolation in porosity waves. Our numerical experiments further indicate that grain‐scale porous flow is more likely in rocks where the melt productivity increases slowly with temperature, such as in metaigneous rocks.
Solar Energetic Particle Events Detected in the Housekeeping Data of the European Space Agency's Spacecraft Flotilla in the Solar System
Authors: Sánchez‐Cano Beatriz , Witasse Olivier , Knutsen Elise W. , Meggi Dikshita , Viet Shayla , Lester Mark , Wimmer‐Schweingruber Robert F. , Pinto Marco , Moissl Richard , Benkhoff Johannes , Opgenoorth Hermann , Auster Uli , de Brujine Jos , Collins Peter , De Marchi Guido , Fischer David , Futaana Yoshifumi , Godfrey James , Heyner Daniel , Holmstrom Mats , Johnstone Andrew , Joyce Simon , Lakey Daniel , Martinez Santa , Milligan David , Montagnon Elsa , Müller Daniel , Livi Stefano A. , Prusti Timo , Raines Jim , Richter Ingo , Schmid Daniel , Schmitz Peter , Svedhem Håkan , Taylor Matt G. G. T. , Tremolizzo Elena , Titov Dimitri , Wilson Colin , Wood Simon , Zender Joe 
Published: July 2023

AbstractDespite the growing importance of planetary Space Weather forecasting and radiation protection for science and robotic exploration and the need for accurate Space Weather monitoring and predictions, only a limited number of spacecraft have dedicated instrumentation for this purpose. However, every spacecraft (planetary or astronomical) has hundreds of housekeeping sensors distributed across the spacecraft, some of which can be useful to detect radiation hazards produced by solar particle events. In particular, energetic particles that impact detectors and subsystems on a spacecraft can be identified by certain housekeeping sensors, such as the Error Detection and Correction (EDAC) memory counters, and their effects can be assessed. These counters typically have a sudden large increase in a short time in their error counts that generally match the arrival of energetic particles to the spacecraft. We investigate these engineering datasets for scientific purposes and perform a feasibility study of solar energetic particle event detections using EDAC counters from seven European Space Agency Solar System missions: Venus Express, Mars Express, ExoMars‐Trace Gas Orbiter, Rosetta, BepiColombo, Solar Orbiter, and Gaia. Six cases studies, in which the same event was observed by different missions at different locations in the inner Solar System are analyzed. The results of this study show how engineering sensors, for example, EDAC counters, can be used to infer information about the solar particle environment at each spacecraft location. Therefore, we demonstrate the potential of the various EDAC to provide a network of solar particle detections at locations where no scientific observations of this kind are available.
Cellular mechanotransduction in health and diseases: from molecular mechanism to therapeutic targets
Authors: Di Xingpeng , Gao Xiaoshuai , Peng Liao , Ai Jianzhong , Jin Xi , Qi Shiqian , Li Hong , Wang Kunjie , Luo Deyi 
Published: July 2023

AbstractCellular mechanotransduction, a critical regulator of numerous biological processes, is the conversion from mechanical signals to biochemical signals regarding cell activities and metabolism. Typical mechanical cues in organisms include hydrostatic pressure, fluid shear stress, tensile force, extracellular matrix stiffness or tissue elasticity, and extracellular fluid viscosity. Mechanotransduction has been expected to trigger multiple biological processes, such as embryonic development, tissue repair and regeneration. However, prolonged excessive mechanical stimulation can result in pathological processes, such as multi-organ fibrosis, tumorigenesis, and cancer immunotherapy resistance. Although the associations between mechanical cues and normal tissue homeostasis or diseases have been identified, the regulatory mechanisms among different mechanical cues are not yet comprehensively illustrated, and no effective therapies are currently available targeting mechanical cue-related signaling. This review systematically summarizes the characteristics and regulatory mechanisms of typical mechanical cues in normal conditions and diseases with the updated evidence. The key effectors responding to mechanical stimulations are listed, such as Piezo channels, integrins, Yes-associated protein (YAP) /transcriptional coactivator with PDZ-binding motif (TAZ), and transient receptor potential vanilloid 4 (TRPV4). We also reviewed the key signaling pathways, therapeutic targets and cutting-edge clinical applications of diseases related to mechanical cues.
Patients with psychosis spectrum disorders hospitalized during the COVID-19 pandemic unravel overlooked SARS-CoV-2 past infection clustering with HERV-W ENV expression and chronic inflammation
Authors: Tamouza Ryad , Meyer Urs , Lucas Alexandre , Richard Jean Romain , Nkam Irène , Pinot Armand , Djonouma Ndilyam , Boukouaci Wahid , Charvet Benjamin , Pierquin Justine , Brunel Joanna , Fourati Slim , Rodriguez Christophe , Barau Caroline , Le Corvoisier Philippe , El Abdellati Kawtar , De Picker Livia , Perron Hervé , Leboyer Marion 
Published: July 2023

AbstractEpidemiology has repeatedly associated certain infections with a risk of further developing psychiatric diseases. Such infections can activate retro-transposable genetic elements (HERV) known to trigger immune receptors and impair synaptic plasticity of neuroreceptors. Since the HERV-W ENV protein was recently shown to co-cluster with pro-inflammatory cytokines in a subgroup of patients with schizophrenia or bipolar disorder, we questioned the influence of the COVID-19 pandemic on patients with psychosis spectrum disorders (PSD). Present results revealed that (i) SARS-CoV-2 serology shows high prevalence and titers of antibodies in PSD, (ii) HERV-W ENV is detected in seropositive individuals only and (iii) SARS-CoV-2 and HERV-W ENV positivity co-clustered with high serum levels of pro-inflammatory cytokines in psychotic patients. These results thus suggest that SARS-CoV-2 infection in many patients with psychotic disorders now admitted in the psychiatry department did not cause severe COVID-19. They also confirm the previously reported association of elevated serum pro-inflammatory cytokines and HERV-W ENV in a subgroup of psychotic patients. In the context of the COVID-19 pandemic, this cluster is only found in SARS-CoV-2 seropositive PSD cases, suggesting a dominant influence of this virus on HERV-W ENV and cytokine expression, and/or patients’ greater susceptibility to SARS-CoV-2 infection. Further investigation on an interplay between this viral infection and the clinical evolution of such PSD patients is needed. However, this repeatedly defined subgroup of psychotic patients with a pro-inflammatory phenotype and HERV expression calls for a differential therapeutic approach in psychoses, therefore for further precision medicine development.
DEF6(differentially exprehomolog) exacerbates pathological cardiac hypertrophy via RAC1
Authors: Sun Yan , Xu Changlu , Jiang Zhongxiu , Jiang Xi 
Published: July 2023

AbstractPathological cardiac hypertrophy involves multiple regulators and several signal transduction pathways. Currently, the mechanisms of it are not well understood. Differentially expressed in FDCP 6 homolog (DEF6) was reported to participate in immunity, bone remodeling, and cancers. The effects of DEF6 on pathological cardiac hypertrophy, however, have not yet been fully characterized. We initially determined the expression profile of DEF6 and found that DEF6 was upregulated in hypertrophic hearts and cardiomyocytes. Our in vivo results revealed that DEF6 deficiency in mice alleviated transverse aortic constriction (TAC)-induced cardiac hypertrophy, fibrosis, dilation and dysfunction of left ventricle. Conversely, cardiomyocyte-specific DEF6-overexpression aggravated the hypertrophic phenotype in mice under chronic pressure overload. Similar to the animal experiments, the in vitro data showed that adenovirus-mediated knockdown of DEF6 remarkably inhibited phenylephrine (PE)-induced cardiomyocyte hypertrophy, whereas DEF6 overexpression exerted the opposite effects. Mechanistically, exploration of the signal pathways showed that the mitogen-activated extracellular signal-regulated kinase 1/2 (MEK1/2)-extracellular signal-regulated kinase 1/2 (ERK1/2) cascade might be involved in the prohypertrophic effect of DEF6. Coimmunoprecipitation and GST (glutathione S-transferase) pulldown analyses demonstrated that DEF6 can directly interact with small GTPase Ras-related C3 botulinum toxin substrate 1 (Rac1), and the Rac1 activity assay revealed that the activity of Rac1 is altered with DEF6 expression in TAC-cardiac hypertrophy and PE-triggered cardiomyocyte hypertrophy. In the end, western blot and rescue experiments using Rac1 inhibitor NSC23766 and the constitutively active mutant Rac1(G12V) verified the requirement of Rac1 and MEK1/2-ERK1/2 activation for DEF6-mediated pathological cardiac hypertrophy. Our study substantiates that DEF6 acts as a deleterious regulator of cardiac hypertrophy by activating the Rac1 and MEK1/2-ERK1/2 signaling pathways, and suggests that DEF6 may be a potential treatment target for heart failure.
NR1D1 deficiency in the tumor microenvironment promotes lung tumor development by activating the NLRP3 inflammasome
Authors: Kim Sun Mi , Jeon Yoon , Jang Ji Yun , Lee Ho 
Published: July 2023

AbstractNuclear receptor Rev-erbα (NR1D1) is a major negative regulator of the circadian clock. Numerous studies have investigated the role of circadian clock-related factors in the tumorigenesis of multiple cancer types, but little is known about the role of NR1D1 in cancer development. In this study, we identified the role of NR1D1 in lung tumorigenesis using genetically engineered mouse models of Nr1d1. Although NR1D1 overexpression or knockdown had little effect on the proliferation of NSCLC cells in vitro, NR1D1 deficiency in the tumor microenvironment increased lung cancer development compared with the control in the orthotopic model. NR1D1-deficient mice showed increased NACHT, LRR, and PYD domain-containing protein 3 (NLRP3) inflammasome activation, and conditioned medium (CM) from NR1D1-deficient macrophages increased the proliferation and epithelial–mesenchymal transition (EMT) of lung cancer cells. Treatment with MCC950, a specific inhibitor of NLRP3 inflammasome, blocked tumorigenesis in NR1D1-deficient mice in an orthotopic lung cancer model. In addition, MCC950 treatment blocked the increased proliferation and EMT of cancer cells induced by CM from NR1D1-deficient macrophages in vitro. Our results showed that NR1D1 in the tumor microenvironment functions as a tumor suppressor by negatively regulating the NLRP3 inflammasome, suggesting that the NLRP3 inflammasome blockade via NR1D1 activation could be a therapeutic strategy to overcome lung cancer.
Engineering tRNA abundances for synthetic cellular systems
Authors: Maheshwari Akshay J. , Calles Jonathan , Waterton Sean K. , Endy Drew 
Published: July 2023

AbstractRoutinizing the engineering of synthetic cells requires specifying beforehand how many of each molecule are needed. Physics-based tools for estimating desired molecular abundances in whole-cell synthetic biology are missing. Here, we use a colloidal dynamics simulator to make predictions for how tRNA abundances impact protein synthesis rates. We use rational design and direct RNA synthesis to make 21 synthetic tRNA surrogates from scratch. We use evolutionary algorithms within a computer aided design framework to engineer translation systems predicted to work faster or slower depending on tRNA abundance differences. We build and test the so-specified synthetic systems and find qualitative agreement between expected and observed systems. First principles modeling combined with bottom-up experiments can help molecular-to-cellular scale synthetic biology realize design-build-work frameworks that transcend tinker-and-test.
Schwann cells regulate tumor cells and cancer-associated fibroblasts in the pancreatic ductal adenocarcinoma microenvironment
Authors: Xue Meilin , Zhu Youwei , Jiang Yongsheng , Han Lijie , Shi Minmin , Su Rui , Wang Liwen , Xiong Cheng , Wang Chaofu , Wang Ting , Deng Shijie , Wu Dong , Cao Yizhi , Dong Lei , Bai Fan , Zhao Shulin , Deng Xiaxing , Peng Chenghong , Li Hongwei , Chen Jianjun , Shen Baiyong , Jiang Lingxi , Chen Hao 
Published: July 2023

AbstractNeuropathy is a feature more frequently observed in pancreatic ductal adenocarcinoma (PDAC) than other tumors. Schwann cells, the most prevalent cell type in peripheral nerves, migrate toward tumor cells and associate with poor prognosis in PDAC. To unveil the effects of Schwann cells on the neuro-stroma niche, here we perform single-cell RNA-sequencing and microarray-based spatial transcriptome analysis of PDAC tissues. Results suggest that Schwann cells may drive tumor cells and cancer-associated fibroblasts (CAFs) to more malignant subtypes: basal-like and inflammatory CAFs (iCAFs), respectively. Moreover, in vitro and in vivo assays demonstrate that Schwann cells enhance the proliferation and migration of PDAC cells via Midkine signaling and promote the switch of CAFs to iCAFs via interleukin-1α. Culture of tumor cells and CAFs with Schwann cells conditioned medium accelerates PDAC progression. Thus, we reveal that Schwann cells induce malignant subtypes of tumor cells and CAFs in the PDAC milieu.
Knowledge-based mechanistic modeling accurately predicts disease progression with gefitinib in EGFR-mutant lung adenocarcinoma
Authors: L’Hostis Adèle , Palgen Jean-Louis , Perrillat-Mercerot Angélique , Peyronnet Emmanuel , Jacob Evgueni , Bosley James , Duruisseaux Michaël , Toueg Raphaël , Lefèvre Lucile , Kahoul Riad , Ceres Nicoletta , Monteiro Claudio 
Published: July 2023

AbstractLung adenocarcinoma (LUAD) is associated with a low survival rate at advanced stages. Although the development of targeted therapies has improved outcomes in LUAD patients with identified and specific genetic alterations, such as activating mutations on the epidermal growth factor receptor gene (EGFR), the emergence of tumor resistance eventually occurs in all patients and this is driving the development of new therapies. In this paper, we present the In Silico EGFR-mutant LUAD (ISELA) model that links LUAD patients’ individual characteristics, including tumor genetic heterogeneity, to tumor size evolution and tumor progression over time under first generation EGFR tyrosine kinase inhibitor gefitinib. This translational mechanistic model gathers extensive knowledge on LUAD and was calibrated on multiple scales, including in vitro, human tumor xenograft mouse and human, reproducing more than 90% of the experimental data identified. Moreover, with 98.5% coverage and 99.4% negative logrank tests, the model accurately reproduced the time to progression from the Lux-Lung 7 clinical trial, which was unused in calibration, thus supporting the model high predictive value. This knowledge-based mechanistic model could be a valuable tool in the development of new therapies targeting EGFR-mutant LUAD as a foundation for the generation of synthetic control arms.
Limits to the accurate and generalizable use of soundscapes to monitor biodiversity
Authors: Sethi Sarab S. , Bick Avery , Ewers Robert M. , Klinck Holger , Ramesh Vijay , Tuanmu Mao-Ning , Coomes David A. 
Published: July 2023

AbstractAlthough eco-acoustic monitoring has the potential to deliver biodiversity insight on vast scales, existing analytical approaches behave unpredictably across studies. We collated 8,023 audio recordings with paired manual avifaunal point counts to investigate whether soundscapes could be used to monitor biodiversity across diverse ecosystems. We found that neither univariate indices nor machine learning models were predictive of species richness across datasets but soundscape change was consistently indicative of community change. Our findings indicate that there are no common features of biodiverse soundscapes and that soundscape monitoring should be used cautiously and in conjunction with more reliable in-person ecological surveys.
Telomere-to-telomere assemblies of 142 strains characterize the genome structural landscape in Saccharomyces cerevisiae
Authors: O’Donnell Samuel , Yue Jia-Xing , Saada Omar Abou , Agier Nicolas , Caradec Claudia , Cokelaer Thomas , De Chiara Matteo , Delmas Stéphane , Dutreux Fabien , Fournier Téo , Friedrich Anne , Kornobis Etienne , Li Jing , Miao Zepu , Tattini Lorenzo , Schacherer Joseph , Liti Gianni , Fischer Gilles 
Published: July 2023

AbstractPangenomes provide access to an accurate representation of the genetic diversity of species, both in terms of sequence polymorphisms and structural variants (SVs). Here we generated theSaccharomyces cerevisiaeReference Assembly Panel (ScRAP) comprising reference-quality genomes for 142 strains representing the species’ phylogenetic and ecological diversity. The ScRAP includes phased haplotype assemblies for several heterozygous diploid and polyploid isolates. We identified circa (ca.) 4,800 nonredundant SVs that provide a broad view of the genomic diversity, including the dynamics of telomere length and transposable elements. We uncovered frequent cases of complex aneuploidies where large chromosomes underwent large deletions and translocations. We found that SVs can impact gene expression near the breakpoints and substantially contribute to gene repertoire evolution. We also discovered that horizontally acquired regions insert at chromosome ends and can generate new telomeres. Overall, the ScRAP demonstrates the benefit of a pangenome in understanding genome evolution at population scale.