bluuebunny/crossref_metadata_2025_split huggingface dataset:
Assessment of the variability of the morphological traits and differentiation of Cucurbita moschata in Cote d’Ivoire
Authors: Seka Dagou , Kouago Badié Arnaud , Bonny Beket Severin 
Published: March 2023

AbstractWith its predisposition to adapt to different environments, Cucurbita moschata grows well in various ecosystems. It is not a very exigent plant and has an inherent capacity for plasticity that underlies its large variability. An assessment of the accessions of C. moschata in Cote d’Ivoire shows that the plant exhibits large morphological and phenological variability for all the 28 measured traits. There are outliers among most measured traits. Further analysis indicates the emergence of three ecotypes in congruence with the three distinct ecosystems and their respective bioclimatic characteristics. In the savannah region, characterized by a short rainy season followed by a long dry season, a total yearly rainfall of 900 mm, an elevated daily temperature of 29 °C, and a high relative humidity of 80%, the cline of C. moschata is long and thin, with small leaves, small peduncles and small fruits. It has a high growth rate and accelerated phenology. The mountain region has a very long rainy season followed by a short dry season, a total pluviometry of 1400 mm, an average daily temperature of 27 °C and a relative humidity of 69%. The cline of C. moschata in the mountain region is characterized by a delayed flowering and a delayed fruit maturity, large number of small seeds and large fruits. The forest region has a favorable climate for the growth of C. moschata in Cote d’Ivoire. It has two rainy seasons that alternate with two dry seasons of unequal durations, an annual rainfall of 1200 mm, an average daily temperature of 27 °C and a relative humidity of 70%. The cline of C. moschata in that region has a large girth size, large dimensions of the leaves, long peduncles and larger and heavier fruits. The seeds are also large but in small number. It appears that the anatomy and physiology of the clines are differentiated primarily in response to soil water content and availability for the ontogeny of the plant.
First record of Jacobsoniidae (Coleoptera) on the African continent in Holocene copal from Tanzania: biogeography since the Cretaceous
Authors: Peris David , Hammel Jörg U. , Cai Chenyang , Solórzano-Kraemer Mónica M. 
Published: March 2023

AbstractNeither fossil nor living Jacobsoniidae are found in abundance. Derolathrus cavernicolus Peck, 2010 is recorded here preserved in Holocene copal from Tanzania with an age of 210 ± 30 BP years. This leads us to three interesting conclusions: (1) This is the first time the family was found on the African continent, extending the family’s distribution range to hitherto unknown localities. Derolathrus cavernicolus in Holocene copal from Tanzania expands the known distribution of the species, previously only recorded in the USA (Hawaii and Florida), Barbados, and Japan, both spatially and temporally. (2) All fossil specimens of the family have been found preserved in amber, which might be due to the small size of the specimens that prevents their discovery in other types of deposits. However, we here add a second aspect, namely the occurrence of this cryptic and currently scarce family of beetles in resinous environments, where they live in relationship with resin-producing trees. (3) The discovery of a new specimen from a family unknown on the African continent supports the relevance of these younger resins in preserving arthropods that lived in pre-Anthropocene times. Although we cannot demonstrate their extinction in the region, since it is possible that the family still survives in the already fragmented coastal forests of East Africa, we are detecting a loss of local biodiversity during the so-called Anthropocene, probably due to human activity.
Myofascial force transmission between the calf and the dorsal thigh is dependent on knee angle: an ultrasound study
Authors: Mohr Lisa , Vogt Lutz , Thiel Christian , Behringer Michael , Wilke Jan 
Published: March 2023

AbstractA recent in-vivo experiment has shown that force can be transmitted between the gastrocnemius and the hamstring muscles due to a direct tissue continuity. However, it remains unclear if this mechanical interaction is affected by the stiffness of the structural connection. This study therefore aimed to investigate the impact of the knee angle on myofascial force transmission across the dorsal knee. A randomized, cross-over study was performed, including n = 56 healthy participants (25.36 ± 3.9 years, 25 females). On two separate days, they adopted a prone position on an isokinetic dynamometer (knee extended or 60° flexed). In each condition, the device moved the ankle three times from maximal plantarflexion to maximal dorsal extension. Muscle inactivity was ensured using EMG. High-resolution ultrasound videos of the semimembranosus (SM) and the gastrocnemius medialis (GM) soft tissue were recorded. Maximal horizontal tissue displacement, obtained using cross-correlation, was examined as a surrogate of force transmission. SM tissue displacement was higher at extended (4.83 ± 2.04 mm) than at flexed knees (3.81 ± 2.36 mm). Linear regression demonstrated significant associations between (1) SM and GM soft tissue displacement (extended: R2 = 0.18, p = 0.001; flexed: R2 = 0.17, p = 0.002) as well as (2) SM soft tissue displacement and ankle range of motion (extended: R2 = 0.103, p = 0.017; flexed: R2 = 0.095, p = 0.022). Our results further strengthen the evidence that local stretching induces a force transmission to neighboring muscles. Resulting remote exercise effects such as increased range of motion, seem to depend on the stiffness of the continuity.Trial registration: DRKS (Deutsches Register Klinischer Studien), registration number DRKS00024420, first registered 08/02/2021, https://drks.de/search/de/trial/DRKS00024420.
Identification of a novel circRNA–miRNA–mRNA regulatory axis in hepatocellular carcinoma based on bioinformatics analysis
Authors: Zhong Guoqiang , Lin Yan , Huang Zansong 
Published: March 2023

AbstractIn recent years, circular RNAs (circRNAs) have been found to play an essential regulatory role in hepatocellular carcinoma (HCC) through various mechanisms, particularly the endogenous competitive RNA (ceRNA) mechanism. Therefore, it is significant to explore the circRNAs in hepatoma. In this study, we constructed the ceRNA and survival network using Cytoscape. We also used R, Perl software, and multiple online databases and platforms, including Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG), to perform overall survival, immune cell infiltration, immune checkpoints, pathway activity, and anticancer drug sensitivity analysis of the genes. Finally, the receiver operator characteristic curve (ROC) analysis was performed to identify the diagnosis value of the genes. KEGG analysis revealed the T cell receptor signaling pathway as the main enrichment pathway. A total of 29 genes related to survival and prognosis were screened out. The findings suggest that ZNF544, WDR76, ACTG1, RASSF3, E2F3, ASRGL1, and POGK are associated with multilevel immune cell infiltration. Additionally, immune checkpoint analysis screened out the ACTG1, E2F3, RASSF3, and WDR76. It was also revealed that the WDR76, E2F3, ASRGL1, and POGK mainly activated the cell cycle and DNA damage response (DDR) pathway. The results suggest that the sensitivity toward trametinib, refametinib (RDEA119), and selumetinib correlates to the expression of WDR76. ROC analysis showed that the area under the curve (AUC) of all genes in the regulatory axis was greater than 0.7. The identified hsa_circ_0000417/hsa_circ_0002688/hsa_circ_0001387--hsa-miR-199a-5p--WDR76 regulatory axis may provide new insights into the progression, clinical diagnosis, and treatment of HCC.
The role of SMAD signaling in hypertrophic obstructive cardiomyopathy: an immunohistopathological study in pediatric and adult patients
Authors: Zhang Zhengjie , Zhang Fengzhi , Zhang Mingkui , Xue Hui , Fan Lixin , Weng Yan 
Published: March 2023

AbstractHypertrophic obstructive cardiomyopathy (HOCM) can bring a high risk of sudden cardiac death in young people. It is particularly urgent to understand the development and mechanism of HOCM to prevent unsafe incidents. Here, the comparison between pediatric and adult patients with HOCM has been performed to uncover the signaling mechanism regulating pathological process through histopathological analysis and immunohistochemical analysis. We found SMAD proteins played an important role during myocardial fibrosis for HOCM patients. In patients with HOCM, Masson and HE staining showed that myocardial cells were diffusely hypertrophied with obvious disorganized myocardial fiber alignment, and myocardial tissue was more damaged and collagen fibers increased significantly, which come early in childhood. Increased expressions of SMAD2 and SMAD3 contributed to myocardial fibrosis in patients with HOCM, which happened early in childhood and continued through adulthood. In addition, decreased expression of SMAD7 was closely related to collagen deposition, which negatively expedited fibrotic responses in patients with HOCM. Our study indicated that the abnormal regulation of SMAD signaling pathway can lead to severe myocardial fibrosis in childhood and its fibrogenic effects persist into adulthood, which is a crucial factor in causing sudden cardiac death and heart failure in HOCM patients.
Mapping the immune landscape in small cell lung cancer by analysing expression of immuno-modulators in tissue biopsies and paired blood samples
Authors: Dutta Rimlee , Rathor Amber , Sharma Hanuman Prasad , Pandey Hem Chandra , Malik Prabhat Singh , Mohan Anant , Nambirajan Aruna , Kumar Rajeev , Jain Deepali 
Published: March 2023

AbstractSmall cell lung carcinomas (SCLC) are aggressive tumors with high propensity to metastasize. Recent NCCN guidelines have incorporated immunotherapy in extensive stage SCLC. Limited benefit in few patients compounded by side effects of unwonted immune-checkpoint-inhibitor (ICPI) usage necessitates identification of potential biomarkers predicting response to ICPIs. Attempting this, we analysed expression of various immunoregulatory molecules in tissue biopsies and paired blood samples of SCLC patients. In 40 cases, immunohistochemistry for expression of immune inhibitory receptors CTLA-4, PD-L1 and IDO1 was performed. Matched blood samples were quantified for IFN-γ, IL-2, TNF-α and sCTLA-4 levels using immunoassay and additionally for IDO1 activity (Kynurenine/Tryptophan ratio) using LC–MS. Immunopositivity for PD-L1, IDO1 and CTLA-4 was identified in 9.3%, 6.2% and 71.8% cases, respectively. Concentration of serum IFN-γ (p-value < 0.001), TNF-α (p-value = 0.025) and s-CTLA4 (p-value = 0.08) were higher in SCLC patients while IL-2 was lower (p-value = 0.003) as compared to healthy controls. IDO1 activity was significantly elevated in SCLC cohort (p-value = 0.007). We proffer that SCLC patients show immune suppressive milieu in their peripheral circulation. Analysis of CTLA4 immunohistochemical expression along with s-CTLA4 levels appears prospective as biomarkers for predicting responsiveness to ICPIs. Additionally, evaluation of IDO1 appears cogent both as prognostic marker and potential therapeutic target as well.
Integrated multi-omics analysis of Alzheimer’s disease shows molecular signatures associated with disease progression and potential therapeutic targets
Authors: Kodam Pradeep , Sai Swaroop R. , Pradhan Sai Sanwid , Sivaramakrishnan Venketesh , Vadrevu Ramakrishna 
Published: March 2023

AbstractAlzheimer’s disease (AD) is a progressive neurodegenerative disease characterized by the formation of amyloid plaques implicated in neuronal death. Genetics, age, and sex are the risk factors attributed to AD. Though omics studies have helped to identify pathways associated with AD, an integrated systems analysis with the available data could help to understand mechanisms, potential biomarkers, and therapeutic targets. Analysis of transcriptomic data sets from the GEO database, and proteomic and metabolomic data sets from literature was performed to identify deregulated pathways and commonality analysis identified overlapping pathways among the data sets. The deregulated pathways included those of neurotransmitter synapses, oxidative stress, inflammation, vitamins, complement, and coagulation pathways. Cell type analysis of GEO data sets showed microglia, endothelial, myeloid, and lymphoid cells are affected. Microglia are associated with inflammation and pruning of synapses with implications for memory and cognition. Analysis of the protein-cofactor network of B2, B6, and pantothenate shows metabolic pathways modulated by these vitamins which overlap with the deregulated pathways from the multi-omics analysis. Overall, the integrated analysis identified the molecular signature associated with AD. Treatment with anti-oxidants, B2, B6, and pantothenate in genetically susceptible individuals in the pre-symptomatic stage might help in better management of the disease.
A multicenter phase II trial of anti-EGFR-immunoliposomes loaded with doxorubicin in patients with advanced triple negative breast cancer
Authors: Mamot Christoph , Wicki Andreas , Hasler-Strub Ursula , Riniker Salome , Li Qiyu , Holer Lisa , Bärtschi Daniela , Zaman Khalil , von Moos Roger , Dedes Konstantin J. , Boos Laura A. , Novak Urban , Bodmer Alexandre , Ritschard Reto , Obermann Ellen C. , Tzankov Alexandar , Ackermann Christoph , Membrez-Antonioli Véronique , Zürrer-Härdi Ursina , Caspar Clemens B. , Deuster Stefanie , Senn Martin , Winterhalder Ralph , Rochlitz Christoph 
Published: March 2023

AbstractAdvanced triple negative breast cancer (TNBC) is an aggressive, but initially chemo-sensitive disease. The prognosis is poor and more than three quarters of patients experience progression 12 months after the initiation of conventional first-line chemotherapy. Approximately two thirds of TNBC express epidermal growth factor receptor 1 (EGFR). We have developed an anti-EGFR targeted nanocontainer drug by inserting anti-EGFR antibody fragments into the membrane of pegylated liposomes (anti-EGFR-ILs-dox). The payload consists of doxorubicin, a standard drug for TNBC. In a first-in-human phase I trial in 26 patients with various advanced solid malignancies, anti-EGFR-ILs-dox has shown little toxicity and encouraging efficacy. In this single-arm phase II trial, we assessed the efficacy of anti-EGFR-ILs-dox as first-line therapy in patients with advanced, EGFR + TNBC. The primary endpoint was progression-free survival at 12 months (PFS12m). Secondary endpoints included overall response rate (ORR), duration of response (DOR), time to progression (TTP), overall survival (OS) and adverse events (AEs). 48 patients received anti-EGFR-ILs-dox 50 mg/m2 iv, on day one of a 28 days-cycle until progression. The Kaplan–Meier estimate for PFS12m was 13% (one-sided 90% CI 7%, 95% CI [5%, 25%]), median PFS was 3.5 months (95% CI 1.9, 5.4). The trial has not reached its primary endpoint. There were no new toxicity signals. Based on these results, anti-EGFR-ILs-dox should not be further developed for TNBC. It remains an open question whether anti-EGFR-ILs-dox would offer more opportunities in other EGFR-expressing malignancies, where targeting this receptor has already shown anticancer effects.Trial registration: This trial was registered at clinicaltrials.gov: NCT02833766. Registered 14/07/2016.
Entropic analysis of cilia-modulated slip flow of trimetallic nanofluid through electroosmotic corrugated pump in the presence of inclined magnetic field
Authors: Munawar Sufian , Saleem Najma , Afzal Farkhanda , Mehmood Arif , Awan Malik Khurram Shahzad , Kumam Poom 
Published: March 2023

AbstractAn incredible eradication of thermal indulgence is required to enhance the flow and heat transfer enhancement in micro/nanofluidic devices. In addition, the rapid transport and instantaneous mixing of colloidal suspensions of metallic particles at nanoscale are exceptionally crucial at ascendency of inertial and surface forces. To address these challenges, the present work is intended to investigate the role of trimetallic nanofluid comprising of three kinds of nano-sized granules (titanium oxide, Silica and Aluminium dioxide) with pure blood through a heated micropump in the presence of inclined magnetic field and axially implemented electric field. To ensure rapid mixing in unidirectional flow, the pump internal surface is lined-up with mimetic motile cilia with slip boundary. The embedded cilia whip in pattern due to dynein molecular motion controlled by time and produce a set of metachronal waves along the pump wall. The shooting technique is executed to compute the numerical solution. In a comparative glance it is revealed that the trimetallic nanofluid exhibits 10% higher heat transfer efficiency as compared to bi-hybrid and mono nanofluids. Moreover, the involvement of electroosmosis results in almost 17% decrease in the heat transfer rate if it values jumps from 1 to 5. The fluid temperature in case of trimetallic nanofluid is higher and thus keeps the heat transfer entropy and the total entropy lower. Furthermore, involvement of thermal radiated and momentum slip significantly contribute in reducing heat losses.
U-Sleep’s resilience to AASM guidelines
Authors: Fiorillo Luigi , Monachino Giuliana , van der Meer Julia , Pesce Marco , Warncke Jan D. , Schmidt Markus H. , Bassetti Claudio L. A. , Tzovara Athina , Favaro Paolo , Faraci Francesca D. 
Published: March 2023

AbstractAASM guidelines are the result of decades of efforts aiming at standardizing sleep scoring procedure, with the final goal of sharing a worldwide common methodology. The guidelines cover several aspects from the technical/digital specifications, e.g., recommended EEG derivations, to detailed sleep scoring rules accordingly to age. Automated sleep scoring systems have always largely exploited the standards as fundamental guidelines. In this context, deep learning has demonstrated better performance compared to classical machine learning. Our present work shows that a deep learning-based sleep scoring algorithm may not need to fully exploit the clinical knowledge or to strictly adhere to the AASM guidelines. Specifically, we demonstrate that U-Sleep, a state-of-the-art sleep scoring algorithm, can be strong enough to solve the scoring task even using clinically non-recommended or non-conventional derivations, and with no need to exploit information about the chronological age of the subjects. We finally strengthen a well-known finding that using data from multiple data centers always results in a better performing model compared with training on a single cohort. Indeed, we show that this latter statement is still valid even by increasing the size and the heterogeneity of the single data cohort. In all our experiments we used 28528 polysomnography studies from 13 different clinical studies.
Towards circular plastics within planetary boundaries
Authors: Bachmann Marvin , Zibunas Christian , Hartmann Jan , Tulus Victor , Suh Sangwon , Guillén-Gosálbez Gonzalo , Bardow André 
Published: March 2023

AbstractThe rapid growth of plastics production exacerbated the triple planetary crisis of habitat loss, plastic pollution and greenhouse gas (GHG) emissions. Circular strategies have been proposed for plastics to achieve net-zero GHG emissions. However, the implications of such circular strategies on absolute sustainability have not been examined on a planetary scale. This study links a bottom-up model covering both the production and end-of-life treatment of 90% of global plastics to the planetary boundaries framework. Here we show that even a circular, climate-optimal plastics industry combining current recycling technologies with biomass utilization transgresses sustainability thresholds by up to four times. However, improving recycling technologies and recycling rates up to at least 75% in combination with biomass and CO2 utilization in plastics production can lead to a scenario in which plastics comply with their assigned safe operating space in 2030. Although being the key to sustainability and in improving the unquantified effect of novel entities on the biosphere, even enhanced recycling cannot cope with the growth in plastics demand predicted until 2050. Therefore, achieving absolute sustainability of plastics requires a fundamental change in our methods of both producing and using plastics.
I-type and S-type granites in the Earth’s earliest continental crust
Authors: Zhong Shihua , Li Sanzhong , Liu Yang , Cawood Peter A. , Seltmann Reimar 
Published: March 2023

AbstractThe composition and origin of Earth’s earliest continental crust remains enigmatic due to the absence of Hadean (>4 Ga) age rocks. Here we address this question by using machine learning to examine the provenance of the 4.4–3.3 Ga Jack Hills zircons, which constitute the best archive of Earth’s earliest continental crust. Our results reveal that although some Jack Hills zircons may be derived from trondhjemite-tonalite-granodiorite series rocks, which were common during the Archean (4–2.5 Ga), most (as high as ~70%) are sourced from igneous (I-) and sedimentary (S-) type granites. This finding provides clear evidence for rocks other than the trondhjemite-tonalite-granodiorite suite in the Earth’s earliest continental crust. Considering that I- and S-type granites are typical of modern convergent plate margins, the presence of a high proportion of Jack Hills zircons from these rocks supports the operation of a horizontal, mobile-lid tectonic regime in the early Earth.
Hydrogen/deuterium exchange-mass spectrometry of integral membrane proteins in native-like environments: current scenario and the way forward
Authors: Javed Waqas , Griffiths Damon , Politis Argyris 
Published: March 2023

AbstractIntegral membrane proteins (IMPs) perform a range of diverse functions and their dysfunction underlies numerous pathological conditions. Consequently, IMPs constitute most drug targets, and the elucidation of their mechanism of action has become an intense field of research. Historically, IMP studies have relied on their extraction from membranes using detergents, which have the potential to perturbate their structure and dynamics. To circumnavigate this issue, an array of membrane mimetics has been developed that aim to reconstitute IMPs into native-like lipid environments that more accurately represent the biological membrane. Hydrogen/deuterium exchange-mass spectrometry (HDX-MS) has emerged as a versatile tool for probing protein dynamics in solution. The continued development of HDX-MS methodology has allowed practitioners to investigate IMPs using increasingly native-like membrane mimetics, and even pushing the study of IMPs into the in vivo cellular environment. Consequently, HDX-MS has come of age and is playing an ever-increasingly important role in the IMP structural biologist toolkit. In the present mini-review, we discuss the evolution of membrane mimetics in the HDX-MS context, focusing on seminal publications and recent innovations that have led to this point. We also discuss state-of-the-art methodological and instrumental advancements that are likely to play a significant role in the generation of high-quality HDX-MS data of IMPs in the future.