bluuebunny/crossref_metadata_2025_split huggingface dataset:
Women disproportionately shoulder burdens imposed by the global COVID‐19 pandemic
Authors: Dinella Lisa M. , Evans Kiameesha , Levinson Jordan A. , Gagnon Samantha 
Published: August 2023

AbstractThe current study focused on how the sudden onset of the pandemic magnified existing inequalities for women in the United States. A total of 2115 participants responded to an online survey regarding pandemic‐related changes to household and childcare responsibilities, employment, mental and physical health and safety, housing, worries and stress, and coping strategies. We employ an intersectionality analytical framework to understand how existing systems of oppression differentially impacted women's lived experiences during the early stages of the pandemic in the United States. Particularly, we investigated how gender, race/ethnicity, and class intersected to impact women's adaptability to the pandemic crisis. We also included motherhood status as a possible variable that may change women's pandemic‐related experiences. Finally, we include women's narrative responses to provide context to their quantitative responses and to help fully represent perspectives that can often be rendered invisible. We leveraged the findings of the current investigation of the impact of the COVID‐19 global pandemic on women's lives to make suggestions for changes that can support women with this and future pandemics and disasters.
Meloidogyne arenaria infecting Matricaria chamomilla in Brazil
Authors: Pazdiora Paulo Cesar , de Ramos Eduarda Kalena Kirsch , Pereira Matheus Martins , Dias Alvaro Renato Guerra , Conce Marianela Conce , de Araujo Filho Jeronimo Vieira 
Published: August 2023

AbstractPlants of Matricaria chamomilla L. (Asteraceae), well‐known medicinal species, showing root galls, were collected in Pelotas, state of Rio Grande do Sul (Brazil). Based on morphological (perineal patterns and males), morphometric (males) and esterase phenotypes (Est A2), we identified the infecting nematode as the root‐knot nematode M. arenaria. Under greenhouse conditions, four M. chamomilla plants were inoculated with about 1000 specimens obtained from the isolate. We observed about 207 ± 72 nematodes g−1 of roots, confirming its pathogenicity. To the best of our knowledge, this is the first record of M. arenaria parasitizing M. chamomilla in Brazil and elsewhere.
Idiopathic hypersomnia years after the diagnosis
Authors: Šonka Karel , Feketeová Eva , Nevšímalová Soňa , Horvat Eszter Maurovich , Příhodová Iva , Dostálová Simona , Galušková Karolína , Milata Martin , Bušková Jitka , Susta Marek 
Published: August 2023

SummaryLittle attention has been paid to the long‐term development of idiopathic hypersomnia symptoms and idiopathic hypersomnia comorbidities. The aim of this study was to describe the general health of patients with idiopathic hypersomnia years after the initial diagnosis, focusing on current subjective hypersomnolence and the presence of its other possible causes. Adult patients diagnosed with idiopathic hypersomnia ≥ 3 years ago at sleep centres in Prague and Kosice were invited to participate in this study. A total of 60 patients were examined (age 47.3 ± SD = 13.2 years, 66.7% women). In all participants, their hypersomnolence could not be explained by any other cause but idiopathic hypersomnia at the time of diagnosis. The mean duration of follow‐up was 9.8 + 8.0 years. Fifty patients (83%) reported persisting hypersomnolence, but only 33 (55%) had no other disease that could also explain the patient's excessive daytime sleepiness and/or prolonged sleep. In two patients (3%), the diagnosis in the meantime had changed to narcolepsy type 2, and 15 patients (25%) had developed a disease or diseases potentially causing hypersomnolence since the initial diagnosis. Complete hypersomnolence resolution without stimulant treatment lasting longer than 6 months was reported by 10 patients (17%). To conclude, in a longer interval from the diagnosis of idiopathic hypersomnia, hypersomnolence may disappear or may theoretically be explained by another newly developed disease, or the diagnosis may be changed to narcolepsy type 2. Thus, after 9.8 years, only 55% of the examined patients with idiopathic hypersomnia had a typical clinical picture of idiopathic hypersomnia without doubts about the cause of the current hypersomnolence.
Longitudinal relations between interpartner aggression and internalizing symptoms among couples: The moderating role of sleep
Authors: Kelly Ryan J. , Gillis Brian T. , El‐Sheikh Mona 
Published: August 2023

SummaryRecipients of interpartner aggression often experience internalizing symptoms. However, individual differences exist, and elucidation of factors that attenuate or exacerbate risk are needed to explicate relations and better inform interventions aimed at reducing mental health sequelae of interpartner aggression. Sleep problems compromise coping abilities and are known to exacerbate risk for mental health problems in the context of family risk. We examined whether sleep problems moderated the extent to which the recipients of interpartner aggression experience internalizing symptoms over time. At the first wave, 194 couples participated (M age [women] = 41.81 years, SD = 5.85; M age [men] = 43.75 years, SD = 6.74; 71% White/European American, 26% Black/African American, 3% other race/ethnicity). Two years later, couples returned for a second wave. Psychological and physical forms of interpartner aggression were measured using self‐ and partner‐reports. Sleep duration (minutes) and sleep quality (efficiency) were derived using actigraphy, and subjective sleep/wake problems were also assessed. Individuals self‐reported on their own internalizing symptoms. After controlling for autoregressive effects, sleep moderated the extent to which the recipients of interpartner aggression experienced internalizing symptoms longitudinally. Lower sleep efficiency and more subjective sleep/wake problems among women exacerbated the extent to which interpartner aggression forecasted their internalizing symptoms. Lower sleep efficiency among men magnified relations between interpartner aggression and their internalizing symptoms. Findings help understand the multiplicative influence that family risk and sleep problems have on mental health over time.
A sleep stage estimation algorithm based on cardiorespiratory signals derived from a suprasternal pressure sensor
Authors: Cerina Luca , Overeem Sebastiaan , Papini Gabriele B. , van Dijk Johannes P. , Vullings Rik , van Meulen Fokke , Ross Marco , Cerny Andreas , Anderer Peter , Fonseca Pedro 
Published: August 2023

SummaryAutomatic estimation of sleep structure is an important aspect in moving sleep monitoring from clinical laboratories to people's homes. However, the transition to more portable systems should not happen at the expense of important physiological signals, such as respiration. Here, we propose the use of cardiorespiratory signals obtained by a suprasternal pressure (SSP) sensor to estimate sleep stages. The sensor is already used for diagnosis of sleep‐disordered breathing (SDB) conditions, but besides respiratory effort it can detect cardiac vibrations transmitted through the trachea. We collected the SSP sensor signal in 100 adults (57 male) undergoing clinical polysomnography for suspected sleep disorders, including sleep apnea syndrome, insomnia, and movement disorders. Here, we separate respiratory effort and cardiac activity related signals, then input these into a neural network trained to estimate sleep stages. Using the original mixed signal the results show a moderate agreement with manual scoring, with a Cohen's kappa of 0.53 in Wake/N1–N2/N3/rapid eye movement sleep discrimination and 0.62 in Wake/Sleep. We demonstrate that decoupling the two signals and using the cardiac signal to estimate the instantaneous heart rate improves the process considerably, reaching an agreement of 0.63 and 0.71. Our proposed method achieves high accuracy, specificity, and sensitivity across different sleep staging tasks. We also compare the total sleep time calculated with our method against manual scoring, with an average error of −1.83 min but a relatively large confidence interval of ±55 min. Compact systems that employ the SSP sensor information‐rich signal may enable new ways of clinical assessments, such as night‐to‐night variability in obstructive sleep apnea and other sleep disorders.
Density effects on a tropical copepod Acartia sp.: Implications as live feed in aquaculture
Authors: Pham Hung Quoc , Van Bui Canh , Doan Nam Xuan , Dinh Khuong V. 
Published: August 2023

AbstractCalanoid copepods Acartia species are major live feeds for the early stages of economically important marine fish in hatcheries in Southeast Asian countries. However, rearing Acartia copepods at high densities to increase productivity remains a major challenge. To address the issue, we conducted two experiments on (1) Acartia sp. nauplii (1000, 3000, 6000, 9000, 12,000, and 15,000 individuals L−1) and (2) adults (1000, 1500, 2000, and 2500 individuals L−1). We assessed key parameters for biomass production: development, survival, and egg and nauplii production. In general, increased density resulted in longer development time, lowered survival and egg and nauplii production but did not affect the size of adult males and females. Despite survival to adulthood decreasing at higher stocking nauplii densities, the number of surviving adults was highest at a stocking density of 12,000 individuals L−1. Egg and nauplii production was very low which may be the result of high predation. The total eggs and nauplii harvested were highest at the lowest adult density. These results are essential for the biomass production of Acartia sp. in central Vietnam.
Individual and population‐level variation in susceptibility to temperature in early life history stages of giant kelp
Authors: Kurman Melissa D. , terHorst Casey 
Published: August 2023

AbstractBecause foundation species create structure in a community, understanding their ecological and evolutionary responses to global change is critical for predicting the ecological and economic management of species and communities that rely on them. Giant kelp (Macrocystis pyrifera) is a globally distributed foundation species with seasonal fluctuations in abundance in response to local nutrient levels, storm intensity, and ocean temperatures. Here we examine genetic variation in individual and population‐level responses of early life history stages (zoospore settlement, survival, and gametogenesis) to increased temperatures to determine the potential for natural selection on temperature‐tolerant individuals that would allow adaptation to a changing climate. We collected fertile M. pyrifera sporophyll blades from three sites along the California coast (Los Angeles, Santa Barbara, Monterey Bay) and induced zoospore release in the lab. Spores settled on microscope slides at three treatment temperatures (16, 20, and 22°C), matured for 21 days, and were imaged weekly to determine settlement, survival, and maturation success. On average, individuals from all sites showed lower rates of settlement and maturation in response to increasing temperature. However, the magnitude of the responses to temperature varied among populations. Survival tended to increase with temperature in Los Angeles and Santa Barbara populations but decreased with increasing temperature for the Monterey Bay population. We observed little genetic variation in temperature responses among individuals within sites, suggesting little scope for evolution within populations to increase the resilience of M. pyrifera populations to warming ocean temperatures and predicted declines in kelp abundance. Yet sufficient dispersal among populations could allow for adaptation of early life history traits among populations via evolutionary rescue of declining populations.
The mystery remains: How do potyviruses move within and between cells?
Authors: Xue Mingshuo , Arvy Nathalie , German‐Retana Sylvie 
Published: August 2023

AbstractThe genus Potyvirus is considered as the largest among plant single‐stranded (positive‐sense) RNA viruses, causing considerable economic damage to vegetable and fruit crops worldwide. Through the coordinated action of four viral proteins and a few identified host factors, potyviruses exploit the endomembrane system of infected cells for their replication and for their intra‐ and intercellular movement to and through plasmodesmata (PDs). Although a significant amount of data concerning potyvirus movement has been published, no synthetic review compiling and integrating all information relevant to our current understanding of potyvirus transport is available. In this review, we highlight the complexity of potyvirus movement pathways and present three potential nonexclusive mechanisms based on (1) the use of the host endomembrane system to produce membranous replication vesicles that are targeted to PDs and move from cell to cell, (2) the movement of extracellular viral vesicles in the apoplasm, and (3) the transport of virion particles or ribonucleoprotein complexes through PDs. We also present and discuss experimental data supporting these different models as well as the aspects that still remain mostly speculative.
Arabidopsis Tubby domain‐containing F‐box proteins positively regulate immunity by modulating PI4Kβ protein levels
Authors: Thulasi Devendrakumar Karen , Copeland Charles , Adamchek Christopher , Zhong Xionghui , Huang Xingchuan , Gendron Joshua M. , Li Xin 
Published: August 2023

Summary
The Tubby domain, named after the TUBBY protein in mice, binds to phosphatidylinositol 4,5‐bisphosphate. Arabidopsis has 11 Tubby domain‐containing proteins referred to as Tubby‐Like Proteins (TLPs). Of the 11 TLPs, 10 possess the N‐terminal F‐box domain, which can interact with SKP‐like proteins and form SKP1‐Cullin‐F‐box E3 ligase complexes. Although mice TUBBY has been extensively studied, plant TLPs' functions are scarcely detailed.
In this study, we show that the Arabidopsis Tubby‐like protein 6 (TLP6) and its redundant homologs, TLP1, TLP2, TLP5, and TLP10, positively regulate Arabidopsis immune responses. Furthermore, in an immunoprecipitation mass spectrometry analysis to search for ubiquitination substrates of the TLPs, we identified two redundant phosphoinositide biosynthesis enzymes, phosphatidylinositol 4‐kinase β proteins (PI4Kβs), PI4Kβ1 and PI4Kβ2, as TLP interactors.
Importantly, TLP6 overexpression lines fully phenocopy the phenotypes of the pi4kβ1,2 mutant, while TLP6 overexpression also leads to increased PI4Kβ2 ubiquitination and reduction in its protein level in a proteasome‐dependent manner. Most significantly, TLP6 overexpression does not further enhance the autoimmunity of the pi4kβ1,2 double mutant, supporting the hypothesis that TLP6 targets the PI4Kβs for ubiquitination and degradation.
Thus, our study reveals a novel mechanism where TLPs promote plant immune responses by modulating the PI4Kβs protein levels.
Persulfidation maintains cytosolic G6PDs activity through changing tetrameric structure and competing cysteine sulfur oxidation under salt stress in Arabidopsis and tomato
Authors: Wang Xiaofeng , Shi Cong , Hu Yanfeng , Ma Ying , Yi Yuying , Jia Honglei , Li Fali , Sun Haotian , Li Tian , Wang Xiuyu , Li Tianjinhong , Li Jisheng 
Published: August 2023

Summary
Glucose‐6‐phosphate dehydrogenases (G6PDs) are essential regulators of cellular redox. Hydrogen sulfide (H2S) is a small gasotransmitter that improves plant adaptation to stress; however, its role in regulating G6PD oligomerization to resist oxidative stress remains unknown in plants.
Persulfidation of cytosolic G6PDs was analyzed by mass spectrometry (MS). The structural change model of AtG6PD6 homooligomer was built by chemical cross‐linking coupled with mass spectrometry (CXMS). We isolated AtG6PD6C159A and SlG6PDCC155A transgenic lines to confirm the in vivo function of persulfidated sites with the g6pd5,6 background.
Persulfidation occurs at Arabidopsis G6PD6 Cystine (Cys)159 and tomato G6PDC Cys155, leading to alterations of spatial distance between lysine (K)491‐K475 from 42.0 Å to 10.3 Å within the G6PD tetramer. The structural alteration occurs in the structural NADP+ binding domain, which governs the stability of G6PD homooligomer. Persulfidation enhances G6PD oligomerization, thereby increasing substrate affinity. Under high salt stress, cytosolic G6PDs activity was inhibited due to oxidative modifications. Persulfidation protects these specific sites and prevents oxidative damage.
In summary, H2S‐mediated persulfidation promotes cytosolic G6PD activity by altering homotetrameric structure. The cytosolic G6PD adaptive regulation with two kinds of protein modifications at the atomic and molecular levels is critical for the cellular stress response.
Effects of yoga on physical and psychological health among community‐dwelling older adults: A systematic review and meta‐analysis
Authors: Ko Ka‐yan , Kwok Zoe Ching Man , Chan Helen Yue‐Lai 
Published: August 2023

AbstractObjectivesTo synthesise and critically appraise the quality of existing evidence about the effects of yoga on physical and psychological outcomes among older adults.DesignA systematic review and meta‐analysis.ParticipantsParticipants aged 60 and above.MeasurementsNine English and two Chinese electronic bibliographic databases, including MEDLINE OvidSP, PsycINFO, AMED, EMBASE, Global Health, PubMed, Scopus, CINAHL, Cochrane Library, Hyread and WanFang databases, were searched. Randomised controlled trials (RCT) of yoga on physical and psychological outcomes among older adults were included. Meta‐analysis was conducted for outcomes studied in three or more studies, otherwise narrative synthesis was performed.ResultsFifteen studies were included for review. The results of meta‐analysis after sensitivity analysis showed that yoga significantly improved balance (SMD = 0.81, 95% CI: 0.48–1.14, p < .001, II2 = 59%), flexibility (SMD = 0.38, 95% CI: 0.07–0.68, p = .020, II2 34%), muscle strength (SMD = 0.49, 95% CI: 0.18–0.79, p = .002, I2 43%) and depressive symptoms (SMD = 0.50, 95% CI = 0.09–0.91, p = .01, I2 0%). There was insufficient data for meta‐analysis on gait and other psychological outcomes. Narrative synthesis suggested that yoga has positive effects on reducing anxiety, but its effects on improving gait and reducing stress among older adults were inconclusive.ConclusionThe results of this review supported the beneficial effects of yoga on physical outcomes among older adults, but the heterogeneity across studies was relatively high. Evidence related to the effect on psychological outcomes was limited.
Associations between systemic melatonin and human myopia: A systematic review
Authors: Hussain Azfira , Gopalakrishnan Aparna , Scott Hannah , Seby Chris , Tang Victoria , Ostrin Lisa , Chakraborty Ranjay 
Published: August 2023

AbstractPurposeExperimental models have implicated the role of melatonin circadian rhythm disruption in refractive error development. Recent studies have examined melatonin concentration and its diurnal patterns on refractive error with equivocal results. This systematic review aimed to summarise the literature on melatonin circadian rhythms in myopia.Recent FindingsPubMed, EMBASE, Web of Science, Scopus, ProQuest Central, LILACS, Cochrane and Medline databases were searched for papers between January 2010 and December 2022 using defined search terms. Seven studies measured melatonin and circadian rhythms in three biological fluids (blood serum, saliva and urine) in both myopes and non‐myopes. Morning melatonin concentrations derived from blood serum varied significantly between studies in individuals aged 10–30 years, with a maximum of 89.45 pg/mL and a minimum of 5.43 pg/mL using liquid chromatography and mass spectrometry. The diurnal variation of salivary melatonin was not significantly different between myopes and emmetropes when measured every 4 h for 24 h and quantified with enzyme‐linked immunosorbent assay. Significantly elevated salivary melatonin concentrations were reported in myopes compared with emmetropes, aged 18–30 years when measured hourly from evening until their habitual bedtime using liquid chromatography. However, the relationship between dim light melatonin onset and refractive group was inconsistent between studies. The 6‐sulphatoxymelatonin concentration derived from overnight urine volume, measured using a double antibody radioimmunoassay, was found to be significantly lower in myopes (29.17 pg/mL) than emmetropes (42.51 pg/mL).SummaryThe role of melatonin concentration and rhythm in myopia has not been studied extensively. This systematic review confirms conflicting findings across studies, with potential relationships existing. Future studies with uniform methodological approaches are required to ascertain the causal relationship between melatonin dysregulation and myopia in humans.
Natural polymorphisms in ZmIRX15A affect water‐use efficiency by modulating stomatal density in maize
Authors: Zhang Kun , Xue Ming , Qin Feng , He Yan , Zhou Yuyi 
Published: August 2023

SummaryStomatal density (SD) is closely related to crop drought resistance. Understanding the genetic basis for natural variation in SD may facilitate efforts to improve water‐use efficiency. Here, we report a genome‐wide association study for SD in maize seedlings, which identified 18 genetic variants that could be resolved to seven candidate genes. A 3‐bp insertion variant (InDel1089) in the last exon of Zea mays (Zm) IRX15A (Irregular xylem 15A) had the most significant association with SD and modulated the translation of ZmIRX15A mRNA by affecting its secondary structure. Dysfunction of ZmIRX15A increased SD, leading to an increase in the transpiration rate and CO2 assimilation efficiency. ZmIRX15A encodes a xylan deposition enzyme and its disruption significantly decreased xylan abundance in secondary cell wall composition. Transcriptome analysis revealed a substantial alteration of the expression of genes involved in stomatal complex morphogenesis and drought response in the loss‐of‐function of ZmIRX15A mutant. Overall, our study provides important genetic insights into the natural variation of leaf SD in maize, and the identified loci or genes can serve as direct targets for enhancing drought resistance in molecular‐assisted maize breeding.
Unfolded protein response pathway in leishmaniasis: A review
Authors: Edirisinghe Nimesha Madhushani , Manamperi Nuwani Harshamali , Wanasinghe Vishmi Samudika , Karunaweera Nadira 
Published: August 2023

AbstractAlteration in the physiological state of the endoplasmic reticulum (ER) leads to the specific response known as unfolded protein response (UPR) or ER stress response. The UPR is driven by three sensor proteins, namely: Inositol‐Requiring Enzyme 1, Protein Kinase RNA‐like ER kinase and Activating Transcription Factor 6 to restore ER homeostasis. Pathogenic infection can initiate UPR activation; some pathogens can subvert the UPR to promote their survival and replication. Many intracellular pathogens, including Leishmania, can interact and hijack ER for their survival and replication, triggering ER stress and subsequently ER stress response. This review aims to provide a comprehensive overview of the ER stress response in infections with the Leishmania species.