bluuebunny/crossref_metadata_2025_split huggingface dataset:
Implementation research priorities for addressing the maternal health crisis in the United States: Results from a modified Delphi study
Authors: Hamm Rebecca F , Moniz Michelle , Wahid Inaya , Breman Rachel B , Callaghan-Koru Jennifer 
Published: February 2023

Abstract
Background: Maternal health outcomes in the United States are far worse than peer nations. Increasing implementation research in maternity care is critical to addressing quality gaps and unwarranted care variations. Implementation research priorities have not yet been defined or well represented in the plans for maternal health research investments in the United States.
Methods:This descriptive study used a modified Delphi method to solicit and rank research priorities at the intersection of implementation science and maternal health. A purposeful, yet broad sample of researchers with relevant subject matter knowledge was identified through searches of published articles and grant databases. Through two sequential web-based surveys, participants submitted and ranked implementation research topics with five areas specific to implementation research: practices to prioritize for (1) broader implementation or (2) de-implementation, and research questions about (3) determinants of implementation, (4) implementation strategies, and (5) research methods/measures.
Results:Eighty-two researchers, predominantly female (90%) and white (75%), volunteered to participate. Sixty completed at least one of two surveys. The practices that participants prioritized for broader implementation were improved postpartum care, perinatal and postpartum mood disorder screening and management, and standardized management of hypertensive disorders of pregnancy. For de-implementation, practices believed to be most impactful if removed from or reduced in maternity care were cesarean delivery for low-risk patients and routine discontinuation of all psychiatric medications during pregnancy. The top methodological priorities of participants were improving the extent to which implementation science frameworks and measures address equity and developing approaches for involving patients in implementation research.
Conclusions: Through a web-based Delphi exercise, we identified implementation research priorities that researchers consider to have the greatest potential to improve the maternity care quality in the United States. This study also demonstrates the feasibility of using modified Delphi approaches to engage researchers in setting implementation research priorities within a clinical area.
Structural variation among assembled genomes facilitates development of rapid and low-cost NOR-linked markers and NOR-telomere junction mapping in Arabidopsis
Authors: Saradadevi Gargi Prasad , Fultz Dalen , Ramgopal Murali Krishna , Subramanian Abirami T , Prince Gerin , Thakur Vivek , Mohannath Gireesha 
Published: February 2023

Abstract
Bioinformatic alignment of the assembled genomes of Col-0 and Sha ecotypes of Arabidopsis thalianarevealed ~ 10,000 genome-wide structural variants involving simple insertions or deletions and repeat contractions or expansions. Using some of these structural variants, we developed new, rapid, and low-cost PCR-based molecular markers that are genetically linked to the nucleolus organizer regions (NORs). A. thaliana has two NORs, one each on chromosome 2 (NOR2) and chromosome 4 (NOR4). Both NORs are ~ 4 Mb each, and hundreds of 45S ribosomal RNA (rRNA) genes are tandemly arrayed at these loci. Using previously characterized recombinant inbred lines (RILs) derived from Col-0 x Sha crosses, we validated the utility of the newly developed NOR-linked markers in genetically mapping rRNA genes and the associated telomeres to either NOR2 or NOR4. Lastly, we sequenced Sha genome using Oxford Nanopore Technology (ONT) and used the data to obtain sequences of NOR-telomere junctions, and with the help of RILs, we mapped them as new genetic markers to their respective NORs (NOR2-TEL2N and NOR4-TEL4N). The structural variants obtained from this study would serve as valuable data for genome-wide association studies (GWAS), developing synteny, and to rapidly design more genome-wide genetic (molecular) markers for new gene/trait mapping purposes.
Cooling channel free surface optimisation for additively manufactured casting tools
Authors: Zeng Tongyan , Abo-Serie Essam , Henry Manus , Jewkes James 
Published: February 2023

Abstract

In the present study, an algorithm has been developed using the adjoint method to optimise the position and cross-section of an internal cooling channel for a 3D printed tool steel insert for use in the Aluminium die-casting process. The algorithm enables the development of an optimised complex industrial mould with relatively low computational cost. A transient model is validated against multiple experimental trials, providing an adapted interface heat transfer coefficient. A steady state thermal model, based on the casting cycle and thermal behaviour at the mould surface, is developed to evaluate the spatial distribution of temperature and to serve as the initial solution for the subsequent optimisation stage. The adjoint model is then applied to optimise the cooling channel emphasising the minimisation of the temperature standard deviation for the mould surface. The original transient model is applied to the optimised mould configuration via calibration using experimental data obtained from a dedicated aluminium furnace. The optimised cooling channel geometry, which uses a non-uniform cross-section across the entire pipe surface region, improves the pressure drop and cooling uniformity across the mould/cast interface by 24.2% and 31.6%, respectively. The model has been used to optimise cooling channels for a range of industrial high-pressure aluminium die-casting (HPADC) inserts. This has yielded a significant improvement in the mould operational lifetime, rising to almost 130,000 shots compared to 40,000 shots for prior designs.
Stereocomplexed microparticles loaded with Salvia cadmica Bioss. extracts for enhancement of immune response towards Helicobacter pylori
Authors: Gonciarz Weronika , Chmiela Magdalena , Kost Bartłomiej , Piątczak Ewelina , Brzeziński Marek 
Published: February 2023

Abstract
Introduction
Controlled delivery of therapeutic substance gives numerous advantages (prevents degradation, improves uptake, sustains concentration, lowers side effects).
Aim
To encapsulate Salviae cadmica extracts (root or aerial part), enriched with polyphenols with immunomodulatory activity, in stereocomplexed microparticles (sc-PLA), resistant to the acidic and basic pH, for using them to enhance the immune response towards gastric pathogen Helicobacter pylori.
Material and methods
Microparticles were made of biodegradable poly(lactic acid) (PLA) and poly(lactic-co-glycolic acid) (PLGA). Stereocomplexation method was used to improve scPLA stability in acidic/basic pH, and to release extracts in pH 8.0, which is in the gut being the center of mucosal immunity.
Results
The obtained polymers are safe in vitro and in vivo (guinea pig model). The sc-PLA modifications enable the release of S. cadmica extracts in pH 8.0. S. cadmica extracts enhanced the phagocytic activity of guinea pig bone marrow derived macrophages, which was diminished by H. pylori, and neutralized H. pylori driven enhanced production of tumor necrosis factor (TNF)-α and interleukin (IL)-10.
Concusion
The sc-PLA encapsulated S. cadmica extracts can be recommended for further in vivo study in guinea pigs infected with H. pylori to confirm their ability to improve an immune response towards this pathogen.
Prognostic significance of albuminuria in elderly of various ages with diabetes
Authors: Hwang Subin , Lee Kyungho , Park Jeeeun , Kim Do Hee , Jeon Junseok , Jang Hye Ryoun , Hur Kyu Yeon , Kim Jae Hyeon , Huh Wooseong , Kim Yoon-Goo , Lee Jung Eun 
Published: February 2023

Abstract
It remains uncertain whether albuminuria can identify elderly patients with diabetes at a high risk of incident end-stage kidney disease (ESKD) or mortality.
3,065 patients (aged ≥65 years) with type 2 diabetes were included. We examined the association between albuminuria stages (normoalbuminuria, A1; microalbuminuria, A2; and macroalbuminuria, A3) and the risk of incident ESKD and all-cause mortality for each age group (65–69, 70–74, and ≥75 years).
A2 and A3 were observed in 25.5% and 9.4% of the subjects, respectively. For A1, A2, and A3, the probabilities of ESKD at 8 years were 1.0%, 6.3%, and 29.7% (P<0.001 for all), and the all-cause mortality was 13.1%, 27.4%, and 31.7% (P<0.001 for A1 vs A2, P<0.001 for A1 vs A3), respectively. Albuminuria stages were independently associated with an increased risk of ESKD [fully adjusted hazard ratios (HR): 3.650 (1.987–6.702) for A2, 10.404 (5.706–18.972) for A3 vs. A1]. The HRs of all-cause mortality were 1.742 (1.411–2.153) for A2 and 1.810 (1.344–2.441) for A3. The associations between albuminuria stages and the risk of ESKD and all-cause mortality were consistent across all age groups.
Microalbuminuria is also a risk factor for incident ESKD and mortality in elderly patients with diabetes.
Progressive Supranuclear Palsy drives the Infratentorial Cerebrovascular Diseases-associated Hypertrophic Olivary Degeneration
Authors: Wang Xuejing , Zhu Qingyong , Wang Jiuqi , Liang Dongxiao , Wang Binghao , Ma Mingming , Ding Xuebing 
Published: February 2023

Abstract
Hypertrophic olivary degeneration (HOD) is a transsynaptic degeneration characterized by the disruption of dentato-rubro-olivary tract, a region also known as the Guillain-Mollaret triangle (GMT), which often occurs because of posterior fossa or brainstem lesions. Infratentorial cerebrovascular diseases (ICVD) has been linked to HOD in previous studies. The underlying mechanism of ICVD patients developed HOD, however, remains undetermined. In this study, we analyzed clinical features of 334 patients with ICVD and the results showed that brainstem hemorrhage was most likely to develop HOD (43.5%) among four types of ICVD. In addition, multivariate regression analysis revealed that PSP-RS was an independent risk factor (OR = 6.69, 95% CI: 1.58–28.32) for ICVD-HOD, and the presence of HOD was obviously higher in PSP-RS patients complicated by ICVD (43.5%) than that in PSP-RS patients uncomplicated by ICVD (4.2%). Furthermore, DTI study showed that the PSP-RS, HOD, PSP-RS with HOD, and PSP-RS with ICVD-HOD groups displayed the impaired microstructural integrity of the GMT compared to iPD and HCs groups. Moreover, some DTI parameters of the GMT showed correlation with the Progressive Supranuclear Palsy Rating Scale (PSPRS) score in PSP-RS patients. Taken together, our study demonstrated that PSP-RS was an independent etiological factor for ICVD-HOD which might due to impaired microstructural integrity of the GMT. More importantly, these findings suggest that PSP-RS patients should aim for the prevention of ICVD to reduce the occurrence of HOD.
An integrated network pharmacology and RNA-seq approach for exploring the renal protection of quercetin on attenuating Ang II- induced cells apoptosis
Authors: Zhang Xiuli , Li Jiapeng , Wu Meizhu , Wu Jinkong , He Shuyu , Lu Yao , Ding Qihang , Wen Ying , Long Linzi , Fu Changgeng , Ali Farman , Shen Aling , Peng Jun 
Published: February 2023

Abstract
Quercetin exerts antihypertensive effects, while its role on hypertensive renal injury remain unknown. Network pharmacology analysis identified multiple potential candidate targets (including TP53, Bcl-2 and BaX) and enriched signaling pathways (including apoptosis and p53 signaling pathway). Hematoxylin and eosin staining revealed that quercetin treatment reduced the pathological changes in renal tissues of Ang II infused mice. RNA sequencing identified quercetin treatment significantly reversed 464 DETs and enriched several signaling pathway (including apoptosis and p53 pathways). Terminal deoxynucleotidyl transferase (TdT) dUTP nick-end labeling staining and Annexin V staining revealed that quercetin treatment reduced cell apoptosis in renal tissues of Ang II-infused mice and in NRK-52E cells stimulated with Ang II. Furthermore, immunohistochemistry and western-blotting indicated that quercetin treatment alleviated the upregulation of p53, BaX, cleaved-caspase-9, and cleaved-caspase-3 protein expression and the downregulation of Bcl-2 protein expression in both renal tissue of Ang II infused mice and NRK-52E cells stimulated with Ang II stimulation. Moreover, the molecular docking results indicated potential binding activity between quercetin-TP53. Quercetin treatment significantly attenuated hypertensive renal injury and cell apoptosis in renal tissues of Ang II-induced mice and Ang II stimulated NERK-52E cell, and by targeting p53 may be one of the underlying mechanisms.
A methodology for evaluation of the dimensional quality of 3D printed parts
Authors: Bowoto Oluwole Kingsley 
Published: February 2023

Abstract
In this study, the dimensional variation defect between the CAD designed model and a 3D printed model using material extrusion technique was investigated by a software algorithm developed. This software analyses the CAD and sliced model to scans for dimensional data, which can be used as a reference to check dimensional deviations during the actual 3D printing operation. It is worthy of note that despite the wide adoption of 3D printing technology in various industries, defects such as dimensional variations hinder its mass production potential. There has been a spike in the adoption of 3D printing technology across various industries due to increased industrial research and development. However, not quite a lot has been done regarding the dimensional accuracy of printed components as this affects the usage of printed components across its various areas of application. In lieu of this, a software algorithm was developed for this study which investigates the dimensional deviations of the printed model through the utilization of computer vision algorithm. This solution will be applicable to a wide range of three dimensional geometries to be printed and hence will anticipate dimensional variance, which could lead to a failed printing, thereby saving economic and human resources in additive manufacturing.
Determining Groundwater Quality for Drinking and Irrigation Purposes using Various Indices and Finding out Parameters Responsible for the Pollution of Groundwater at a Stretch of Tons Riverin Uttarakhand using Factor Analysis
Authors: Pant Shikha , Dumka Basant Ballabh 
Published: February 2023

Abstract
A study was conducted to determine whether groundwater is suitable for drinking or not in the part of Tons River Basin, which is located at Dehradun in Uttarakhand. Groundwater samples from sixteen locations were collected in May 2012 and May 2019 by Central Ground Water Board at Dehradun. Fifteen physico-chemical parameters were evaluated for these groundwater samples using standard methods of groundwater quality analysis. In this study the parameters responsible for the pollution of groundwater at the sampling site were investigated using factor analysis. Also, the groundwater quality for drinking and irrigation purposes were assessed using water quality index, seven irrigation water quality parameters and criteria given by Richard, Wilcox, Wescot & Ayers.
TRAF3 Suppression Encourages B Cell Recruitment and Prolongs Survival of Microbiome-Intact Mice with Ovarian Cancer
Authors: Zorea Jonathan , Motro Yair , Mazor Roei D. , Carmi Yifat Koren , Shulman Ziv , Mahajna Jamal , Moran-Gilad Jacob , Elkabets Moshe 
Published: February 2023

Abstract
Background
Ovarian cancer (OC) has proven to be the most deadly of all gynecologic cancers. Clinical trials involving the use of immunotherapies in OC patients have produced disappointing outcomes, underscoring the necessity of identifying new immunomodulatory targets for the treatment of this cancer.
Methods
We conducted an in-vivo CRISPR screen of immunodeficient (NSG) and immune-intact wild type (WT) C57/BL6 mice to identify tumor-derived immune-escape mechanisms in a BRAC1- and TP53-deficient murine ID8 OC cell line (designated ITB1). To confirm gene expression and signaling pathway activation in ITB1 cells, we employed western blot, qPCR, immunofluorescent staining, and flow cytometry. Flow cytometry was also used to identify immune cell populations in the peritoneum of ITB1-bearing mice. To determine the presence of IgA-coated bacteria in the peritoneum of ITB1-bearing mice and the ascites of OC patients, we employed 16S sequencing.Testing for differences was done by using Deseq2 test and two-way ANOVA test. Sequence variants (ASVs) were produced in Qiime2 and analyzed by microeco and phyloseq R packages.
Results
We identified tumor necrosis factor receptor-associated factor 3 (TRAF3) as a tumor-derived immune suppressive mediator in ITB1 cells. Knockout of TRAF3 (TRAF3KO) activated the type-I interferon pathway and increased MHC-I expression. TRAF3KO tumors exhibited a growth delay in WT mice vs. NSG mice, which was correlated with increased B cell infiltration and activation compared to ITB1 tumors. B cells were found to be involved in the progression of TRAF3KO tumors, and B-cell surface-bound and secreted IgA levels were significantly higher in the ascites of TRAF3KO tumors compared to ITB1. The presence of commensal microbiota was necessary for B-cell activation and for delaying the progression of TRAF3KO tumors in WT mice. Lastly, we observed unique profiles of IgA-coated bacteria in the ascites of OC-bearing mice or the ascites of OC patients.
Conclusions
TRAF3 is a tumor-derived immune-suppressive modulator that influences B-cell infiltration and activation, making it a potential target for enhancing anti-tumor B-cell responses in OC.
Multidimensional analysis and prediction of E-commerce sales based on convolutional neural network
Authors: Bao Jie 
Published: February 2023

Abstract
At present, artificial intelligence methods such as machine learning are widely used in E-commerce enterprises, but the disconnection between business practice and prediction technology is still a real challenge for E-commerce enterprises. Firstly, this paper focuses on the actual business of E-commerce enterprises, carries on a multi-dimensional analysis of the influencing factors of E-commerce sales, refines various factors affecting E-commerce sales, further summarizes the feature construction work of E-commerce sales prediction, constructs the feature project of sales prediction, and provides reference for the practical application of E-commerce enterprises. Secondly, an E-commerce sales forecasting model based on Convolutional Neural Network (CNN) is proposed. The model adopts the feature learning of CNN's Alexnet and integrates the attention mechanism. Finally, based on the data of E-commerce enterprises, this paper compares the prediction effects of other conventional machine learning models. The experimental results show that the CNN based fusion prediction model proposed in this paper can improve the accuracy rate, have better prediction performance, and provide an effective in-depth learning method for E-commerce management.
Cyclosporine A alleviates colitis by inhibiting the formation of neutrophil extracellular traps via regulating the pentose phosphate pathway
Authors: Xu Chenjing , Ye Ziping , Jiang Wenyu , Wang Shu , Zhang Hongjie 
Published: February 2023

Abstract
Background: The aberrant formation of neutrophil extracellular traps (NETs) has been implicated in ulcerative colitis (UC), a chronic recurrent intestinal inflammation. Cyclosporine A (CsA) is now applied as rescue therapy for acute severe UC. In addition, it has been curtained that CsA inhibits the formation of NETs in vitro and the mechanism of which was still vague. The study aimed to explore the mechanism CsA inhibits the NETs formation of colitis in vivo and in vitro.
Methods: NETs enrichment in clinical samples was analyzed using databases from Gene Expression Omnibus and verified in our center. Dextran sulfate sodium (DSS)-induced acute colitis mice model was used to investigate the effect of CsA on NETs of colonic tissue expression. To clarify the mechanism, intracellular energy metabolites were examined by Liquid Chromatograph Mass Spectrometer, and ROS levels were examined by fluorescence intensity in neutrophils treated with CsA after LPS stimulation. The transcriptional level and activity of G6PD of neutrophils were also assessed using qRT-PCR and WST-8. RNA-sequence was used to detect differentially expressed genes of neutrophils stimulated by LPS with or without CsA. The expression levels of related proteins were detected by western blot.
Results: NETs enrichment was especially elevated in moderate-to-severe UC patients compared to HC. NETs expression in the colon from DSS colitis was decreased after CsA treatment. Compared with neutrophils stimulated by LPS, NETs formation and cellular ROS levels were decreased in LPS+CsA group. Cellular guanosine monophosphate and NADPH/NADP+ related to the pentose phosphate pathway (PPP) were reduced in LPS+CsA group. In addition, CsA could decrease G6PD（rate-limited enzyme of PPP） activity in neutrophils stimulated with LPS, and the results were further verified by inhibiting G6PD activity. At last, P53 protein was highly expressed in LPS+CsA group compared with the LPS group. Intracellular G6PD activity, ROS level and NETs formation, which were downregulated by CsA, could be reversed by a P53 inhibitor.
Conclusion: Our results indicated CsA could alleviate the severity of colitis by decreasing the formation of NETs in vivo. In vitro, CsA reduced NETs release via downregulating PPP and cellular ROS levels by decreasing G6PD activity directly by activating the P53 protein.
Experimental investigation of cutting performance for circular saw blade body modification by resin layers toward green manufacturing
Authors: Kang Jinyou , Zhang Jinsheng , Sun Depeng , Song Weiye 
Published: February 2023

Abstract
Cemented carbide circular saw blades are widely used in the metal and nonmetal manufacturing industry for cut-off and cut-tail production. High energy consumption and intense noise emission from conventional-type blades are urgent environmental issues to be solved. Hence, a laminated-resin blade is proposed to study the effect of adding a damping structure to the blade body on its cutting performance. Apart from reduced energy consumption, lower noise and high-quality surfaces are required by manufacturers. In this work, a laminated-resin blade and a conventional blade were compared regarding the details of cutting force and energy consumption, noise emission, and surface integrity. In addition, the effect of cutting fluid on conventional saw blades was analyzed in terms of cutting force and noise emission. Experimental results show that the cutting force and noise of the laminated-resin blade were less than that of the conventional one, indicating reductions of 27.8% and 18 dB, respectively. Especially a reduction of more than 20 dB sound level was achieved in the high-frequency band. Lower cutting force attained by the laminated-resin blade showed that smoother cuts could be achieved. Cutting with cutting fluid is superior to dry cutting in terms of cutting force, noise level, and workpiece surface integrity. The research sheds new light on the design of damping structures on saw blade bodies and quantitative analysis of the cutting performance.