bluuebunny/crossref_metadata_2025_split huggingface dataset:
Jagged/Notch proteins promote endothelial‐mesenchymal transition‐mediated pulmonary arterial hypertension via upregulation of the expression of GATAs
Authors: Lin Kun‐Chen , Yeh Jui‐Ning , Shao Pei‐Lin , Chiang John Y. , Sung Pei‐Hsun , Huang Chi‐Ruei , Chen Yi‐Ling , Yip Hon‐Kan , Guo Jun 
Published: March 2023

AbstractThis study tested the hypothesis that Jagged2/Notches promoted the endothelial‐mesenchymal transition (endMT)‐mediated pulmonary arterial hypertension (PAH) (i.e. induction by monocrotaline [MCT]/63 mg/kg/subcutaneous injection) through increasing the expression of GATA‐binding factors which were inhibited by propylthiouracil (PTU) (i.e. 0.1% in water for daily drinking since Day 5 after PAH induction) in rodent. As compared with the control (i.e. HUVECs), the protein expressions of GATAs (3/4/6) and endMT markers (Snail/Zeb1/N‐cadherin/vimentin/fibronectin/α‐SMA/p‐Smad2) were significantly reduced, whereas the endothelial‐phenotype markers (CD31/E‐cadherin) were significantly increased in silenced JAG2 gene or in silenced GATA3 gene of HUVECs (all p < 0.001). As compared with the control, the protein expressions of intercellular signallings (GATAs [3/4/6], Jagged1/2, notch1/2 and Snail/Zeb1/N‐cadherin/vimentin/fibronectin/α‐SMA/p‐Smad2) were significantly upregulated in TGF‐ß/monocrotaline‐treated HUVECs that were significantly reversed by PTU treatment (all p < 0.001). By Day 42, the results of animal study demonstrated that the right‐ventricular systolic‐blood‐pressure (RVSBP), RV weight (RVW) and lung injury/fibrotic scores were significantly increased in MCT group than sham‐control (SC) that were reversed in MCT + PTU groups, whereas arterial oxygen saturation (%) and vasorelaxation/nitric oxide production of PA exhibited an opposite pattern of RVW among the groups (all p < 0.0001). The protein expressions of hypertrophic (ß‐MHC)/pressure‐overload (BNP)/oxidative‐stress (NOX‐1/NOX‐2) biomarkers in RV and the protein expressions of intercellular signalling (GATAs3/4/6, Jagged1/2, notch1/2) and endMT markers (Snail/Zeb1/N‐cadherin/vimentin/fibronectin/TGF‐ß/α‐SMA/p‐Smad2) in lung parenchyma displayed an identical pattern of RVW among the groups (all p < 0.0001). Jagged‐Notch‐GATAs signalling, endMT markers and RVSBP that were increased in PAH were suppressed by PTU.
Comparison accuracy of digital and analog method using milled occlusal splints
Authors: Blasi Álvaro , Henarejos‐Domingo Víctor , Palacios‐Bañuelos Ricardo , Aparicio Conrado , Roig Miguel 
Published: March 2023

AbstractObjectiveThis clinical trial aims to compare the accuracy of interocclusal registration in centric relation taken with polyvinyl siloxane and intraoral scanner (IOS) with the fabrication of occlusal devices.MethodsThirty‐one participants were included in the trial registered at ClinicalTrials.gov (NCT05317182) receiving two different occlusal devices from two different workflows. One workflow was performed using polyvinyl siloxane impression material and the other with IOS. Every splint was scanned before and after the occlusal adjustments to compare the volumetric changes using the Root Mean Square deviation (RMS). Furthermore, three evaluators assessed the 3D comparison using color maps in a Visual Analog Scale (VAS).ResultsThe average values of RMS were higher for the analog approach (0.01 ± 0.067) than the digital approach (0.065 ± 0.035). However, the differences were not statistically significant (p < 0.063) between the two impression techniques. For the semiquantitative analysis performed by blinded evaluators, differences in VAS values between the impression for the digital (2.08 ± 2.4) and analog (3.80 ± 3.3) technique were statistically significant. The three evaluators agreed in more than 90% of the qualitative dichotomous evaluation.ConclusionDigital impressions did not show inferior accuracy compared to conventional impressions when assessed using quantitative measurements.Clinical SignificanceThis clinical trial provided evidence on registering interocclusal relationship at increased vertical dimension with fully digital workflow for complete arch prosthesis.
The evaluation and downscaling‐calibration of IMERG precipitation products at sub‐daily scales over a metropolitan region
Authors: Zhuang Qi , Zhou Zhengzheng , Liu Shuguang , Wright Daniel B. , Gao Lisha 
Published: March 2023

AbstractThe Global Precipitation Measurement (GPM) Integrated Multi‐satellitE Retrievals for GPM products (i.e., IMERG) provide new‐generation satellite precipitation measurements. For urban contexts, however, the issues of its bias and insufficient resolutions still exist. This study aims to develop high‐precision and high‐resolution (e.g., 0.01°/1 h) data for a metropolitan region based on IMERG and gauge precipitations. The original IMERG product is evaluated using hourly in situ precipitations from 47 gauges. A spatial downscaling‐calibration (DC) technique is then developed to enhance the IMERG using the Normalized Difference Vegetation Index. The results show the limited capability of IMERG to capture sub‐daily precipitation and high‐intensity precipitation. The proposed DC method significantly improves IMERG performance, with correlation coefficient (CC) increasing from 0.07 to 0.75, and probability of detection improving from 0.34 to 0.90 at the hourly scale. In terms of spatial rainfall distribution, 86% of mean absolute error and 80% of RMSE are improved with CC increasing from 0.07 to 0.91 on average. Additionally, the calibrated downscaled product provides finer information in local areas, capturing three times more spatial variabilities of urban precipitation against the original IMERG input data. The results highlight the necessity of improving urban observations for flood risk management at fine spatiotemporal resolutions.
Assessing the fulfillment of nurses' full potential in diverse geriatric settings—moving towards change
Authors: Romem Anat , Pinchas‐Mizrachi Ronit , Abramovich Nataly , Wagman Shir , Ravid Talya Shahnis , Lizeachin Alla , David Tova , Diamant Tsaffy Hillel , Mekadesh Keren , Goldberg Shoshy , Rozani Violetta 
Published: March 2023

AbstractBackgroundIn response to the aging population, the Department for Professional Development in the Nursing Division at the Israeli Ministry of Health has invested significant resources in training registered nurses for post‐basic certification in the field of geriatrics. However, little is known about how the nurses implement the scope of practice in the field and how they maximize their full potential following the training.Aims and objectivesTo assess whether nurses' full potential is maximized in geriatric acute and long‐term geriatric care, and community settings in Israel.DesignA quantitative, cross‐sectional study.MethodsThe study population is a convenience sample of registered nurses who completed post‐basic certification in geriatric care (n = 287). The participants completed a self‐administered questionnaire addressing socio‐demographic and occupational characteristics, implementation of the scope of practice, and a professional satisfaction questionnaire.Baseline demographic variables were summarized using means and counts, with frequency counts and percentages used for the scope of practice. A one‐way ANOVA was utilized to examine the differences between the workplace and the scope of practice. Chi‐square was used to examine practice types and the workplace.ResultsNurses in all surveyed geriatric settings reported average to high overall performance in independent decision‐making activities and delegated duties. The majority (66%) reported very high satisfaction with the completion of the post basic certification education However overall performance differed significantly between the different geriatric settings.ConclusionMost of the nurses with a post‐basic geriatric certification are satisfied with their scope of practice, feel valued by colleagues and managers, and are able to practice independently within their professional scope. This can have a positive impact on the care of the older adult population.Clinical RelevanceAdministrators should ensure that nurses with a post‐basic geriatric certification are able to maximize their scope of practice. Specifically, we recommend nurse managers to leverage the results of this study and advocate for nurses to fulfill their full potential in their scope of practice.
Morning perception of sleep, stress, and mood, and its relationship with overnight physiological sleep: findings from the National Consortium on Alcohol and Neurodevelopment in Adolescence (NCANDA) study
Authors: Albinni Benedetta , Baker Fiona C. , Javitz Harold , Hasler Brant P. , Franzen Peter L. , Clark Duncan B. , de Zambotti Massimiliano 
Published: March 2023

SummaryThis cross‐sectional study investigated objective–subjective sleep discrepancies and the physiological basis for morning perceptions of sleep, mood, and readiness, in adolescents. Data collected during a single in‐laboratory polysomnographic assessment from 137 healthy adolescents (61 girls; age range: 12–21 years) in the United States National Consortium on Alcohol and Neurodevelopment in Adolescence (NCANDA) study were analysed. Upon awakening, participants completed questionnaires assessing sleep quality, mood, and readiness. We evaluated the relationship between overnight polysomnographic, electroencephalographic, sleep autonomic nervous system functioning measures, and next morning self‐reported indices. Results showed that older adolescents reported more awakenings, yet they perceived their sleep to be deeper and less restless than younger adolescents. Prediction models including sleep physiology measures (polysomnographic, electroencephalographic, and sleep autonomic nervous system) explained between 3% and 29% of morning sleep perception, mood, and readiness indices. The subjective experience of sleep is a complex phenomenon with multiple components. Distinct physiological sleep processes contribute to the morning perception of sleep and related measures of mood and readiness. More than 70% of the variance (based on a single observation per person) in the perception of sleep, mood, and morning readiness is not explained by overnight sleep‐related physiological measures, suggesting that other factors are important for the subjective sleep experience.
Porphyromonas gingivalis triggers inflammation in hepatocyte depend on ferroptosis via activating the NF‐κB signaling pathway
Authors: Yao Chao , Lan Dongmei , Li Xue , Wang Yan , Qi Shengcai 
Published: March 2023

AbstractObjectiveNon‐alcoholic fatty liver disease (NAFLD) is a clinicopathological syndrome characterized by excessive fat deposition in hepatocytes caused by non‐alcoholic liver injury. Porphyromonas gingivalis (P.g) is the main pathogen causing periodontitis, which can aggravate the progression of NAFLD in our previously study. The objective of this study was to further investigate the pathogenesis and moleculer michanisma of NAFLD aggravated by P.g.MethodsA mouse model of NAFLD was established, and the changes of inflammatory factors and NF‐κB signaling pathway in liver tissue and L‐02 cells were analyzed by transcriptome sequencing, Western blot, IHC and RT‐PCR. In addition, the NF‐κB signaling pathway inhibitor QNZ and ferroptosis inhibitor Fer‐1 were used to analyze the relationship between NF‐κB signaling pathway and ferroptosis in vitro.ResultsIn vivo and in vitro experiments, P.g can induce liver inflammation and activate NF‐κB signaling pathway. At the same time, P.g promotes ferroptosis and inflammation in L‐02 in vitro. QNZ alleviates ferroptosis and inflammatory activation in L‐02. Fer‐1 can relieve the L‐02 inflammation caused by P.g products.ConclusionPorphyromonas gingivalis can induce ferroptosis and inflammation in hepatocytes and further worsen liver lesions. The mechanism of ferroptosis in hepatocytes depends on NF‐κB signaling pathway, which provides a new strategy for clinical treatment and prevention of NAFLD.
High‐frequency ultrasound point‐of‐care device to quantify myopia‐induced microstructural changes in the anterior sclera
Authors: Hoerig Cameron , Hoang Quan V. , Aichele Johannes , Catheline Stefan , Mamou Jonathan 
Published: March 2023

AbstractPurposeTo develop a point‐of‐care (POC) device using high‐frequency ultrasound (US) for evaluating microstructural changes in the anterior sclera associated with myopia.MethodsThe proposed POC device must satisfy four primary requirements for effective clinical use: the measurement component is handheld; the software must be simple and provide real‐time feedback; patient safety and health data security requirements set forth by relevant governing bodies must be satisfied and the measurement data must have sufficient signal‐to‐noise ratio (SNR) and repeatability. Radiofrequency (RF) echo data acquired by the POC device will be processed using our quantitative US methods to characterise tissue microstructure and biomechanical properties.ResultsAll stated requirements have been met in the developed POC device. The high‐frequency transducer is housed in a custom, 3D‐printed, pen‐like holder that allows for easy measurements of the anterior sclera. Custom software provides a simple interface for data acquisition, real‐time data display and secure data storage. Exposimetry measurements of the US pressure field indicate device compliance with United States Food and Drug Administration limits for ophthalmic US. In vivo measurements on a volunteer suggest the RF data SNR and acquisition consistency are suitable for quantitative analysis.ConclusionsA fully functioning POC device using high‐frequency US has been created for evaluating the microstructure of the anterior sclera. Planned studies using the POC device to scan the eyes of myopia patients will help clarify how the anterior sclera microstructure may be affected by myopia. If effective, this portable, inexpensive and user‐friendly system could be an important part of routine eye examinations.
Pure experience revisited: A critical reassessment of Nishida Kitaro's radicalization of William James' empiricism
Authors: Stone Richard , Altobrando Andrea 
Published: March 2023

AbstractIn this paper, we will revisit the relation between the respective conceptions of pure experience of Nishida Kitaro and William James. As various authors have previously shown, comparing Nishida and James can not only help us better understand both of their specific understandings of pure experience, and consequently its position within their respective enterprises, but also give a platform with which to see how these two authors could contribute to contemporary discussions on philosophical methodology. However, despite the long history of comparative research on these two thinkers, there is still no consensus about the extent to which Nishida's philosophy actually resembles James' work at all. Was Nishida's interest in James' work found only in superficial similarities between two authors interested in overcoming the subject–object distinction? Or did Nishida attempt to somehow develop or radicalize James' work, taking his notion of pure experience into the realm of religion and metaphysics? In this contribution, we will agree with previous authors who have argued that Nishida is best understood as trying to go beyond or develop James' work. However, we will make this claim with the caveat that James had good reason not to take the avenues Nishida pursued, and that Nishida's attempt to radicalize some ideas in James may have led to some difficult points within his early philosophy.
Longitudinal associations among dimensional symptoms of depression and anxiety and first onset suicidal ideation in adolescents
Authors: Metts Allison V. , Echiverri‐Cohen Aileen M. , Yarrington Julia S. , Zinbarg Richard E. , Mineka Susan , Craske Michelle G. 
Published: March 2023

AbstractIntroductionDepression and anxiety are implicated in suicide risk, but the contributionof specific symptom dimensions within these disorders is not well understood. The present study examined longitudinal associations of transdiagnostic symptoms (General Distress[GD]) and unique symptom dimensions (Anhedonia–Apprehension [AA], Fears, and Narrow Depression [ND]) of depression and anxiety and suicidal ideation (SI).MethodsData from 551 adolescents oversampled on high neuroticism were examined in a series of discrete‐time survival analyses to predict first SI onset over an 8‐year period.ResultsResults indicate that GD, AA, and ND were independent predictors of increased likelihood of SI onset and remained significant when controlling for effects of fears. Furthermore, AA and GD remained significant when controlling for one another. ND effects reduced by 24% when adjusting for AA and 74% when adjusting for GD. Fears did not significantly predict SI onset.ConclusionResults suggest that broad levels of distress across depression and anxiety, deficits in positive affect, and elevated negative affect specific to depression increase the likelihood of suicidal thoughts. As such, attention to broader distress and a lack of pleasure, interest, and motivation—potentially more so than negative affect characterizing depression—are particularly important for addressing suicide risk in adolescents.
Detection of a novel papillomaviral sequence in viral plaques confined to the pinna of a dog
Authors: Munday John S. , Orbell Geoff , Robinson Lynne 
Published: March 2023

AbstractA raised plaque that contained histological evidence of papillomavirus infection and sequences from a novel papillomavirus type developed close to the ear canal of a 14‐year‐old West Highland white terrier. The plaque was excised, and further plaques developed within the same area of pinna.
Evaluation of a commercialNGSservice for detection of bacterial and fungal pathogens in infectious ulcerative keratitis
Authors: Bendlin Ashley , Gemensky‐Metzler Anne J. , Diaz‐Campos Dubraska , Newbold Georgina M. , Miller Eric J. , Chandler Heather L. 
Published: March 2023

AbstractObjectivesTo compare results from a commercial next‐generation sequencing (NGS) service to corneal cytology and culture for identification of causative organisms in veterinary patients presenting for infectious ulcerative keratitis (IUK).ProcedureSwabs for corneal aerobic and fungal cultures and DNA swabs for NGS were submitted for canine and equine normal controls (n = 11 andn = 4, respectively) and IUK patients (n = 22 andn = 8, respectively) for which microbrush cytology specimens confirmed the presence of infectious organisms. The sensitivity of the NGS results was compared with bacterial and fungal culture results. Concordance between the NGS and culture results was determined.ResultsThe NGS results were positive for bacterial and fungal organisms in 5 and 1 normal and 18 and 1 IUK cases, respectively. Bacterial and fungal cultures were positive for 7 and 2 normal and 20 and 5 IUK cases, respectively. Sensitivity of NGS was 82.14% (95% confidence interval (CI), 63.11% to 93.94%) and specificity was 76.47% (95% CI, 50.10% to 93.19%). Concordance (complete and partial) between identified bacterial and fungal organisms was found in 79% and 100% of cases, respectively. NGS identified organisms in 3 culture‐negative IUK samples.ConclusionA commercial NGS service may be useful in the identification of causative agents in IUK cases with a sensitivity greater than the sensitivity previously reported for aerobic culture. Further testing is needed to determine the clinical significance of additional organisms isolated by NGS from infected cases, as well as organisms isolated from normal corneas.
Extraction and determination of mercury from spring water, beverage and rice samples using combined microwave‐assisted cloud point and dispersive‐solid phase extraction in micellar media
Authors: Seyedmohammadi Parastoo , Taheri Alireza , Rezayatizad Zeinab 
Published: March 2023

AbstractThe determination of inorganic mercury species in water, beverages and foods has been a special research priority in recent years because mercury, as a highly carcinogenic species, even in trace concentration, has high adverse effects on the body of living organisms. In this research, combining two extraction techniques, including microwave‐assisted cloud point extraction and dispersive‐solid phase extraction (MA‐CP‐dSPE), for the separation and pre‐concentration of mercury in environmental samples is presented. This method is based on the dispersion of nanoparticles in micellar media in the presence of a novel mono‐thiosemicarbazone Schiff base (2 N′‐[4‐diphenylamino]benzylidene) hydrazinecarbothiohydrazide) ligand. Using the experimental conditions defined in the optimization, the method allowed mercury determination from 0.020 to 350 mg/L with a detection limit of 0.005 mg/L. The accuracy of the procedure was tested by analysing certified reference material. The method was successfully applied to copper determination in spring water, beverage and rice samples.
Xenotransplantation: A historical–ethical account of viewpoints
Authors: Rodger Daniel , Hurst Daniel J. , Cooper David KC 
Published: March 2023

AbstractFormal clinical trials of pig‐to‐human organ transplant—known asxenotransplantation—may begin this decade, with the first trials likely to consist of either adult renal transplants or pediatric cardiac transplant patients. Xenotransplantation as a systematic scientific study only reaches back to the latter half of the 20th century, with episodic xenotransplantation events occurring prior to that. As the science of xenotransplantation has progressed in the 20th and 21st centuries, the public's knowledge of the potential therapy has also increased. With this, there have been shifting ethical stances toward xenotransplantation in key areas, such as religious and public viewpoints towards xenotransplantation, animal rights, and public health concerns. This review provides a historical–ethical account of xenotransplantation and details if or how viewpoints have shifted over time.
Stability version of Dirac's theorem and its applications for generalized Turán problems
Authors: Zhu Xiutao , Győri Ervin , He Zhen , Lv Zequn , Salia Nika , Xiao Chuanqi 
Published: March 2023

AbstractIn 1952, Dirac proved that every 2‐connected ‐vertex graph with the minimum degree contains a cycle of length at least . Here we obtain a stability version of this result by characterizing those graphs with minimum degree and circumference at most . We present applications of the above‐stated result by obtaining generalized Turán numbers. In particular, for all we determine how many copies of a five‐cycle as well as four‐cycle are necessary to guarantee that the graph has a circumference larger than . In addition, we give a new proof of Luo's theorem for cliques using our stability result.