bluuebunny/crossref_metadata_2025_split huggingface dataset:
A Novel Fibrinogen Mutation p.BβAla68Asp Causes an Inherited Dysfibrinogenemia
Authors: Jia Kaiqi , Zeng Manlin , Zheng Xiaoyong , Xie Haixiao , Yang Lihong , Xie Yaosheng , Wang Mingshan 
Published: July 2023

Abstract
Objective Our study aimed to analyze the phenotype and genotype of a pedigree with inherited dysfibrinogenemia, and preliminarily elucidate the probable pathogenesis.
Methods The one-stage clotting method was used to test the fibrinogen activity (FIB:C), whereas immunoturbidimetry was performed to quantify the fibrinogen antigen (FIB:Ag). Furthermore, DNA sequence analysis was conducted to confirm the site of mutation. Conservation analysis and protein model analysis were performed using online bioinformatics software.
Results The FIB:C and FIB:Ag of the proband were 1.28 and 2.20 g/L, respectively. Gene analysis revealed a heterozygous c.293C > A (p.BβAla68Asp) mutation in FGB. Bioinformatics and modeling analysis suggested that the missense mutation could potentially have a deleterious effect on fibrinogen.
Conclusion The BβAla68Asp mutation in exon 2 of FGB may account for the reduced FIB:C levels observed in the pedigree. To our knowledge, this point mutation is the first report in the world.
The Risk of Developmental Dysplasia of the Hip in Premature Infants with Breech Presentation at Birth
Authors: Osman Ahmed , Jackson Kenneth , Conroy Sara , Seguin John , Slaughter Jonathan L. 
Published: July 2023

Objective This article evaluates the risk (defined here as incidence per 1,000 infants) of developmental dysplasia of the hip (DDH) in premature infants undergoing hip ultrasound (HUS) for breech presentation only compared with other indications.
Study Design Retrospective study of infants born between January 1, 2009 and December 31, 2018 at <37 weeks' gestation with a HUS obtained in the first year of life, at Nationwide Children's Hospital, the only available facility for pediatric radiology and orthopaedic services in central Ohio. We calculated risk differences and odds ratios of DDH by the indication of HUS.
Results From 2,397 infants reviewed, 89% underwent HUS for breech presentation only. The local incidence of DDH for infants undergoing HUS for breech-only indication was 15 per 1,000 compared with 155 per 1,000 for infants undergoing HUS for other indications. The odds ratio of DDH diagnosis was 12.1 (95% confidence interval: 7.5, 19.6) for infants undergoing HUS for an indication other than breech presentation only relative to infants undergoing HUS for the indication of breech presentation only.
Conclusion The risk of DDH diagnosis in premature infants undergoing HUS for breech presentation is much lower than those undergoing HUS for clinical concerns and other risk factors. Screening these infants with physical examination may be sufficient.
Key Points
Efficacy and Safety of Aspirin 162 mg for Preeclampsia Prophylaxis in High-Risk Patients
Authors: Ayyash Mariam , Goyert Gregory , Garcia Robyn , Khangura Raminder , Pitts D'Angela , Jacobsen Gordon , Shaman Majid 
Published: July 2023

Objective The aim of this study was to compare the safety and efficacy of aspirin 162 mg to the standard recommended dose of 81 mg for preeclampsia prevention.
Study Design A retrospective cohort study of patients at risk for preeclampsia who delivered between January 2013 and December 2020 at Henry Ford Health was performed. Patients were divided into three groups: a no aspirin group, a group treated under an 81 mg aspirin preeclampsia prophylaxis protocol, and a group treated under a 162 mg protocol. Univariate and multivariable logistic regression analyses compared rates of preeclampsia and secondary outcomes between groups. Clinical side effects traditionally associated with aspirin use were also assessed.
Results Of 3,597 patients, 2,266 (63%) were in the no aspirin group, 944 (26%) were in the 81 mg group, and 387 (11%) were in the 162 mg group. The rate of preeclampsia was significantly lower in the 162 mg group (10.1%, odds ratio, 0.68; 95% confidence interval, 0.46–0.99) compared with the 81 mg group (14.2%). The rate of preeclampsia was identical in the no aspirin and 81 mg groups. The rate for postpartum hemorrhage, postpartum hematoma, and intraventricular hemorrhage of the newborn were not significantly different between patients in the 162 and 81 mg groups.
Conclusion We observed a significantly lower rate of preeclampsia in high-risk patients who were treated with the 162 mg dose of aspirin for preeclampsia prophylaxis, and bleeding complications were not seen with the higher dose. Our study suggests that aspirin 162 mg may be considered for prophylaxis in patients at high risk for preeclampsia.
Key Points
The cascade influence of grain trade shocks on countries in the context of the Russia-Ukraine conflict
Authors: Liu Linqing , Wang Weiran , Yan Xiaofei , Shen Mengyun , Chen Haizhi 
Published: July 2023

AbstractThe Russia-Ukraine conflict has severely impacted global food security. This may increase the risk of supply chain disruption in low-income countries that rely heavily on grain imports. This study used production and trade data for wheat, barley and maize from 1995 to 2021 to construct longitudinal trade networks. On this basis, a cascading failure network model of shock propagation was used to identify the direct or indirect dependence of other countries on grain exported from Russia and Ukraine and the impact caused by trade shocks. The results revealed that the interruption of grain exports from Russia and Ukraine has resulted in an increasing impact on the global grain trade year by year and that the wheat trade is the most vulnerable to shock propagation, but it is also the most resilient. Russia and Ukraine interrupt exports of grain, causing more than 50% reduction in direct imports to 30 countries, including Eritrea, Seychelles, Kazakhstan and Mongolia. A shock propagation model that considers indirect dependence yields divergent results, with lower middle income (LM) countries in North Africa, Southeast Asia and West Asia facing supply shocks from reduced imports because they are unable to fully exploit the trade channels to balance grain supply and demand. Under the COVID-19 pandemic, this indirect dependence on imports is more prominent. It is worth noting that Eastern and Southern European countries often act as intermediaries to spread shocks during cascading failures. In the process of shock propagation, the main suppliers of grain include the United States, Canada, France, Argentina and Brazil. After the outbreak of COVID-19, the import demand faced by Australia increased significantly. We also examined how nodal characteristics relate to shock propagation dynamics and country vulnerability, finding that high import diversity, low import dependence and regional characteristics are effective in buffering countries from supply shocks. This study contributes to our understanding of the external supply risks for grain arising from the Russia-Ukraine conflict in a pandemic context, highlights the issue of accessibility in food security and provides trade policy recommendations to mitigate national vulnerability to food insecurity, thereby creating a resilient food trade system.
pHAPE: a plasmid for production of DNA size marker ladders for gel electrophoresis
Authors: Allen Angel G , Barthelson Karissa , Lardelli Michael 
Published: July 2023

Abstract
DNA size markers (also known as ‘molecular weight markers’ or ‘DNA ladders’) are an essential tool when using gel electrophoresis to identify and purify nucleic acids. However, the cost of these DNA ladders is not insignificant and, over time, impinges on the funds available for research and training in molecular biology. Here, we describe a method for the generation of ‘pHAPE’, a plasmid from which a variety of DNA ladders can be generated via simple restriction enzyme digestions. The pHAPE plasmid can be generated by mutagenesis of the commonly used pBluescript II SK+ phagemid followed by insertion of a 7141 bp sequence (comprised of three smaller, synthetic fragments). Our use of pHAPE allows us some small relief from the ever-rising costs of performing molecular biology experiments (‘Don’t worry, pHAPE’).
Provincializing “web traffic”: data imaginaries and vernacular construction ofliuliangin China
Authors: Zou Sheng 
Published: July 2023

AbstractThis article dehomogenizes the concept of “web traffic” through a keywords-informed approach. It attends to how, in China, the term for “web traffic,” liuliang (流量), is utilized in rich and creative ways. Instantiating bottom-up epistemic practices, the taxonomies, collocations, and wisdom related to liuliang reveal alternative and locally relevant ways in which people imagine, apprehend, and deal with digital media and data in everyday life. They unleash a set of reinvigorating vocabulary for the theorization of web traffic—liquidity, manipulability, portability, socio-spatial differentiation, ideological valence, and mystified power, among others. These new lenses enrich a genuinely global understanding of digital media, enable those in the Global North to comparatively rethink their taken-for-granted experiences of datafication, and democratize the making of media knowledges by addressing the inequality between the Global North and South(s), between experts and non-experts.
Artificial intelligence: a new field of knowledge for nephrologists?
Authors: Fayos De Arizón Leonor , Viera Elizabeth R , Pilco Melissa , Perera Alexandre , De Maeztu Gabriel , Nicolau Anna , Furlano Monica , Torra Roser 
Published: July 2023

ABSTRACT
Artificial intelligence (AI) is a science that involves creating machines that can imitate human intelligence and learn. AI is ubiquitous in our daily lives, from search engines like Google to home assistants like Alexa and, more recently, OpenAI with its chatbot. AI can improve clinical care and research, but its use requires a solid understanding of its fundamentals, the promises and perils of algorithmic fairness, the barriers and solutions to its clinical implementation, and the pathways to developing an AI-competent workforce. The potential of AI in the field of nephrology is vast, particularly in the areas of diagnosis, treatment and prediction. One of the most significant advantages of AI is the ability to improve diagnostic accuracy. Machine learning algorithms can be trained to recognize patterns in patient data, including lab results, imaging and medical history, in order to identify early signs of kidney disease and thereby allow timely diagnoses and prompt initiation of treatment plans that can improve outcomes for patients. In short, AI holds the promise of advancing personalized medicine to new levels. While AI has tremendous potential, there are also significant challenges to its implementation, including data access and quality, data privacy and security, bias, trustworthiness, computing power, AI integration and legal issues. The European Commission's proposed regulatory framework for AI technology will play a significant role in ensuring the safe and ethical implementation of these technologies in the healthcare industry. Training nephrologists in the fundamentals of AI is imperative because traditionally, decision-making pertaining to the diagnosis, prognosis and treatment of renal patients has relied on ingrained practices, whereas AI serves as a powerful tool for swiftly and confidently synthesizing this information.
Classroom Discourse: What Is Conveyed Through Educational Interpretation
Authors: Langer Elizabeth C , Crume Peter K 
Published: July 2023

AbstractWhen a deaf or hard-of-hearing child enters a classroom with an interpreter, the goal, and sometimes the assumption, is that they will be granted full access to the classroom experience. This study focuses on the clarity and completeness with which critical elements of classroom discourse are conveyed through the interpretations of 40 educational interpreters. Elements studied include conveyance of main ideas, directions for assignments, relevance strategies, orienting commentary, participation solicitation, mental state reference, and semantic organization. The interpretations clearly and completely conveyed approximately one-third to two-thirds of the information (M = 48.6%) related to these elements of classroom discourse. Frequent omissions and alterations rendered large parts of the message markedly different. Results suggest a need to improve training of educational interpreters, increase communication between teachers and interpreters, provide students supplementary services, and heighten awareness that the interpretation process is fallible in ways that can impact access to classroom discourse.
Trend and early clinical outcomes of off-pump coronary artery bypass grafting in the UK
Authors: Chan Jeremy , Dimagli Arnaldo , Dong Tim , Fudulu Daniel P , Sinha Shubhra , Angelini Gianni D 
Published: July 2023

Abstract

OBJECTIVES
The popularity of off-pump coronary artery bypass grafting (CABG) varies across the world, ranging from 20% in Europe and the USA to 56% in Asia. We present the trend and early clinical outcomes in off pump in the UK.

METHODS
All patients who underwent elective or urgent isolated CABG from 1996 to 2019 were extracted from the National Adult Cardiac Surgery Audit database. The trend in operating surgeons and units volume and training in off pump were analysed. Early clinical outcomes between off- and on-pump CABG were compared using propensity score matching.

RESULTS
A total of 351 422 patients were included. The overall off-pump rate during the study period was 15.17%, it peaked in 2008 (19.8%), followed by a steady decreased to 2018 (7.63%). Its adoption varied across centres and surgeons, ranging from &lt;1% to 48.36% and &lt;1% to 85.5%, respectively, of total cases performed. After propensity score matching for the period 1996–2019, off pump, when compared to on pump, was associated with a lower in-hospital/30-day mortality (1.2% vs 1.5%, P &lt; 0.001), return to theatre (3.7% vs 4.5%, P &lt; 0.001), cerebrovascular accident (transient ischaemic attack: 0.3% vs 0.6%, stroke: 0.3% vs 0.6%, P &lt; 0.001) and deep sternal wound infection (0.8% vs 1.2%, P ≤ 0.001). In a sub-analysis from the introduction of EuroScore II (2012–2019), there were no differences in-hospital/30-day mortality (1.0% vs 1.0%, P = 0.71). However, on pump, had a higher return to theatre (4.2% vs 2.7%, P &lt; 0.001), cerebrovascular accident (transient ischaemic attack: 0.4% vs 0.2%, stroke: 0.5% vs 0.3%, P = 0.003) and deep sternal wound infection (1.0% vs 0.6%, P = 0.004).

CONCLUSIONS
Our data show a decreasing trend in the use of off pump in the UK since 2008. This is likely to be multifactorial and raises the question of whether it should be a specialized revascularization technique.
Anatomic, histologic, and mechanical features of the right atrium: implications for leadless atrial pacemaker implantation
Authors: O’Connor Matthew , Barbero Umberto , Kramer Daniel B , Lee Angela , Hua Alina , Ismail Tevfik , McCarthy Karen P , Niederer Steven , Rinaldi Christopher A , Markides Vias , Clarke John-Ross D , Babu-Narayan Sonya , Ho Siew Yen , Wong Tom 
Published: July 2023

Abstract

Background
Leadless pacemakers (LPs) may mitigate the risk of lead failure and pocket infection related to conventional transvenous pacemakers. Atrial LPs are currently being investigated. However, the optimal and safest implant site is not known.

Objectives
We aimed to evaluate the right atrial (RA) anatomy and the adjacent structures using complementary analytic models [gross anatomy, cardiac magnetic resonance imaging (MRI), and computer simulation], to identify the optimal safest location to implant an atrial LP human.

Methods and results
Wall thickness and anatomic relationships of the RA were studied in 45 formalin-preserved human hearts. In vivo RA anatomy was assessed in 100 cardiac MRI scans. Finally, 3D collision modelling was undertaken assessing for mechanical device interaction. Three potential locations for an atrial LP were identified; the right atrial appendage (RAA) base, apex, and RA lateral wall. The RAA base had a wall thickness of 2.7 ± 1.6 mm, with a low incidence of collision in virtual implants. The anteromedial recess of the RAA apex had a wall thickness of only 1.3 ± 0.4 mm and minimal interaction in the collision modelling. The RA lateral wall thickness was 2.6 ± 0.9 mm but is in close proximity to the phrenic nerve and sinoatrial artery.

Conclusions
Based on anatomical review and 3D modelling, the best compromise for an atrial LP implantation may be the RAA base (low incidence of collision, relatively thick myocardial tissue, and without proximity to relevant epicardial structures); the anteromedial recess of the RAA apex and lateral wall are alternate sites. The mid-RAA, RA/superior vena cava junction, and septum appear to be sub-optimal fixation locations.
Recently formed Antarctic lakes host less diverse benthic bacterial and diatom communities than their older counterparts
Authors: Kollár Jan , Kopalová Kateřina , Kavan Jan , Vrbická Kristýna , Nývlt Daniel , Nedbalová Linda , Stibal Marek , Kohler Tyler J 
Published: July 2023

Abstract
Glacier recession is creating new water bodies in proglacial forelands worldwide, including Antarctica. Yet, it is unknown how microbial communities of recently formed “young” waterbodies (originating decades to a few centuries ago) compare with established “old” counterparts (millennia ago). Here, we compared benthic microbial communities of different lake types on James Ross Island, Antarctic Peninsula, using 16S rDNA metabarcoding and light microscopy to explore bacterial and diatom communities, respectively. We found that the older lakes host significantly more diverse bacterial and diatom communities compared to the young ones. To identify potential mechanisms for these differences, linear models and dbRDA analyses suggested combinations of water temperature, pH, and conductivity to be the most important factors for diversity and community structuring, while differences in geomorphological and hydrological stability, though more difficult to quantify, are likely also influential. These results, along with an indicator species analysis, suggest that physical and chemical constraints associated with individual lakes histories are likely more influential to the assembly of the benthic microbial communities than lake age alone. Collectively, these results improve our understanding of microbial community drivers in Antarctic freshwaters, and help predict how the microbial landscape may shift with future habitat creation within a changing environment.
Genome Dynamics and Temperature Adaptation During Experimental Evolution of Obligate Intracellular Bacteria
Authors: Herrera Paul , Schuster Lisa , Zojer Markus , Na Hyunsoo , Schwarz Jasmin , Wascher Florian , Kempinger Thomas , Regner Andreas , Rattei Thomas , Horn Matthias 
Published: July 2023

Abstract
Evolution experiments with free-living microbes have radically improved our understanding of genome evolution and how microorganisms adapt. Yet there is a paucity of such research focusing on strictly host-associated bacteria, even though they are widespread in nature. Here, we used the Acanthamoeba symbiont Protochlamydia amoebophila, a distant relative of the human pathogen Chlamydia trachomatis and representative of a large group of protist-associated environmental chlamydiae, as a model to study how obligate intracellular symbionts evolve and adapt to elevated temperature, a prerequisite for the pivotal evolutionary leap from protist to endothermic animal hosts. We established 12 replicate populations under two temperatures (20 °C, 30 °C) for 510 bacterial generations (38 months). We then used infectivity assays and pooled whole-genome resequencing to identify any evolved phenotypes and the molecular basis of adaptation in these bacteria. We observed an overall reduction in infectivity of the symbionts evolved at 30 °C, and we identified numerous nonsynonymous mutations and small indels in these symbiont populations, with several variants persisting throughout multiple time points and reaching high frequencies. This suggests that many mutations may have been beneficial and played an adaptive role. Mutated genes within the same temperature regime were more similar than those between temperature regimes. Our results provide insights into the molecular evolution of intracellular bacteria under the constraints of strict host dependance and highly structured populations and suggest that for chlamydial symbionts of protists, temperature adaptation was facilitated through attenuation of symbiont infectivity as a tradeoff to reduce host cell burden.
The long-term effects of genomic selection: 2. Changes in allele frequencies of causal loci and new mutations
Authors: Wientjes Yvonne C J , Bijma Piter , van den Heuvel Joost , Zwaan Bas J , Vitezica Zulma G , Calus Mario P L 
Published: July 2023

Abstract
Genetic selection has been applied for many generations in animal, plant, and experimental populations. Selection changes the allelic architecture of traits to create genetic gain. It remains unknown whether the changes in allelic architecture are different for the recently introduced technique of genomic selection compared to traditional selection methods and whether they depend on the genetic architectures of traits. Here, we investigate the allele frequency changes of old and new causal loci under 50 generations of phenotypic, pedigree, and genomic selection, for a trait controlled by either additive, additive and dominance, or additive, dominance, and epistatic effects. Genomic selection resulted in slightly larger and faster changes in allele frequencies of causal loci than pedigree selection. For each locus, allele frequency change per generation was not only influenced by its statistical additive effect but also to a large extent by the linkage phase with other loci and its allele frequency. Selection fixed a large number of loci, and 5 times more unfavorable alleles became fixed with genomic and pedigree selection than with phenotypic selection. For pedigree selection, this was mainly a result of increased genetic drift, while genetic hitchhiking had a larger effect on genomic selection. When epistasis was present, the average allele frequency change was smaller (∼15% lower), and a lower number of loci became fixed for all selection methods. We conclude that for long-term genetic improvement using genomic selection, it is important to consider hitchhiking and to limit the loss of favorable alleles.