bluuebunny/crossref_metadata_2025_split huggingface dataset:
Primary cardiac mesothelioma presenting with fulminant recurrent pericarditis: a case report
Authors: Schwartzenberg Shmuel , Shapira Yaron , Rubachevski Victor , Sharony Ram 
Published: February 2023

AbstractBackgroundPrimary pericardial mesothelioma is an extremely rare disease. Prognosis is poor, with little effects of chemo- or radio-therapy. The majority of cases is diagnosed at autopsy.Case summaryA 22-year-old man, who presented with recurrent pericarditis and large pericardial effusion 2 months after a second BNT162b2 COVID-19 vaccine, underwent pericardiocentesis and pericardial window. Pathology specimen of pericardium revealed benign mesothelial inflammation, consistent with acute pericarditis. Four months later, he presented with a large pericardial mass manifesting in heart failure and underwent urgent pericardiectomy. A new pathology specimen immunostaining and fluorescence in situ hybridization analysis revealed pericardial mesothelioma. Despite intensive care, the patient died 3 weeks later.DiscussionPrimary pericardial mesothelial should be considered in the differential diagnosis of refractory recurrent pericarditis, even with prior biopsy-proven pericarditis or when a putative trigger (COVID-19 mRNA prior vaccination) is suspected, as was the case in this patient. Tumour diagnosis and identification consist of multimodal imaging and laboratory tests. A multidisciplinary, individualized care approach should be performed.
Postoperative ST-segment elevation caused by mechanical compression of the native right coronary artery by a pericardial drainage tube
Authors: Escherich Teresa , Mogilansky Christian , Massoudy Parwis 
Published: February 2023

Abstract
ST-segment elevation is often the first remarkable sign of postoperative cardiac ischaemia. It requires prompt diagnostic measures and an early treatment to improve the patient’s outcome. We describe a case of early postoperative ST-segment elevation due to mechanical compression of the native right coronary artery by a pericardial drainage tube in a patient after replacement of the ascending aorta.
Promotion of healthy nutrition in primary and secondary cardiovascular disease prevention: a clinical consensus statement from the European Association of Preventive Cardiology
Authors: Vassiliou Vassilios S , Tsampasian Vasiliki , Abreu Ana , Kurpas Donata , Cavarretta Elena , O’Flaherty Martin , Colombet Zoé , Siegrist Monika , De Smedt Delphine , Marques-Vidal Pedro 
Published: February 2023

Abstract

Background
Poor dietary habits are common and lead to significant morbidity and mortality. However, addressing and improving nutrition in various cardiovascular settings remain sub-optimal. This paper discusses practical approaches to how nutritional counselling and promotion could be undertaken in primary care, cardiac rehabilitation, sports medicine, paediatric cardiology, and public health.

Discussion
Nutrition assessment in primary care could improve dietary patterns and use of e-technology is likely to revolutionize this. However, despite technological improvements, the use of smartphone apps to assist with healthier nutrition remains to be thoroughly evaluated. Cardiac rehabilitation programmes should provide individual nutritional plans adapted to the clinical characteristics of the patients and include their families in the dietary management. Nutrition for athletes depends on the sport and the individual and preference should be given to healthy foods, rather than nutritional supplements. Nutritional counselling is also very important in the management of children with familial hypercholesterolaemia and congenital heart disease. Finally, policies taxing unhealthy foods and promoting healthy eating at the population or workplace level could be effective for prevention of cardiovascular diseases. Within each setting, gaps in knowledge are provided.

Conclusion
This clinical consensus statement contextualizes the clinician’s role in nutrition management in primary care, cardiac rehabilitation, sports medicine, and public health, providing practical examples of how this could be achieved.
Diagnostic delay in arrhythmogenic cardiomyopathy
Authors: Tini Giacomo , Graziosi Maddalena , Musumeci Beatrice , Targetti Mattia , Russo Domitilla , Parisi Vanda , Argirò Alessia , Ditaranto Raffaello , Leone Ornella , Autore Camillo , Olivotto Iacopo , Biagini Elena 
Published: February 2023

Abstract

Aims
Diagnosis of arrhythmogenic cardiomyopathy (ACM) may be challenging, as it comprises diverse phenotypes (right dominant, biventricular, and left dominant), and each may overlap with other clinical entities. The issue of differential diagnosis with conditions mimicking ACM has been previously highlighted; however, a systematic analysis of ACM diagnostic delay, and of its clinical implications, is lacking.

Methods and results
Data of all ACM patients from three Italian Cardiomyopathy Referral Centres were reviewed to assess the time from first medical contact to definitive ACM diagnosis; a significant diagnostic delay was defined as a time to ACM diagnosis ≥2 years. Baseline characteristics and clinical course of patients with and without diagnostic delay were compared. Of 174 ACM patients, 31% experienced diagnostic delay, with a median time to diagnosis of 8 years (20% in right-dominant ACM, 33% in left-dominant ACM, and 39% in biventricular). Patients with diagnostic delay, when compared with those without, more frequently exhibited an ACM phenotype with left ventricular (LV) involvement (74 vs. 57%, P = 0.04) and a specific genetic background (none had plakophilin-2 variants). The most common initial (mis)diagnoses were dilated cardiomyopathy (51%), myocarditis (21%), and idiopathic ventricular arrhythmia (9%). At follow-up, all-cause mortality was greater in those with diagnostic delay (P = 0.03).

Conclusion
Diagnostic delay is common in patients with ACM, particularly in the presence of LV involvement, and is associated with greater mortality at follow-up. Clinical suspicion and increasing use of tissue characterization by cardiac magnetic resonance in specific clinical settings are of key importance for the timely identification of ACM.
Routine versus selective near-infrared spectroscopy-guided shunting during carotid eversion endarterectomy
Authors: Kondov Stoyan , Bothe Dominique , Beyersdorf Friedhelm , Czerny Martin , Harloff Andreas , Pooth Jan-Steffen , Kaier Klaus , Schöllhorn Joachim , Kreibich Maximilian , Siepe Matthias , Rylski Bartosz 
Published: February 2023

AbstractOBJECTIVESThe aim of this study was to compare outcomes of routine shunting to near-infrared spectroscopy (NIRS)-guided shunting in patients undergoing eversion endarterectomy (EEA) under general anaesthesia.METHODSWe retrospectively evaluated data of all patients undergoing EEA of the internal carotid artery (ICA) in our department from January 2011 until January 2019. Included were patients with EEA of the ICA and the patients were divided into 2 groups: selective-shunting group and routine-shunting group. Patients (i) with patch angioplasty during the surgery, (ii) undergoing surgery for restenosis and (ii) stenosis after radiation therapy, (iii) without recorded regional cerebral oxygen saturation trends, (iv) presenting with an emergency treatment indication and (v) operated upon by residents were excluded. In all patients, EEA was performed in general anaesthesia and under NIRS monitoring. One-to-one propensity score matching was used to compare EEA outcomes after routine shunting to NIRS-guided shunting. Primary end points were defined as perioperative stroke and in-hospital mortality after EEA.RESULTSRoutine and NIRS-guided selective shunting were applied in 340 (34.0%) and 661 (66.0%) patients, respectively. A total of 277 pairs were generated via propensity score matching. Fifty-eight (20.1%) from the selective-shunting group were intraoperatively shunted. Concomitant procedures were more frequently performed in the routine-shunting group [170 (61.4%) vs 47 (17.0%), 180 (65%) vs 101 (36.5%), and 60 (21.7%) vs 6 (2.2%), P &lt; 0.001]. The perioperative stroke rate in the routine-shunting group was higher as well [11 (4.0%) vs 3 (1.1%), P = 0.022]. In-hospital death was overall 0.2% (n = 1). Multivariable logistic regression in the matched patient indicated age (odds ratio 1.050, 95% confidence interval 1.002–1.104, P = 0.046) and routine shunting (odds ratio 2.788, confidence interval 1.119–7.428, P = 0.032) as risk factors for perioperative stroke during EEA of the ICA.CONCLUSIONSWe found that, during EEA of the ICA, under general anaesthesia, NIRS-guided selective shunting was associated with a lower incidence of perioperative stroke than routine shunting.
Cost-effectiveness of point-of-care interventions to tackle inappropriate prescribing of antibiotics in high- and middle-income countries: a systematic review
Authors: D’hulster Erinn , De Burghgraeve Tine , Luyten Jeroen , Verbakel Jan Y 
Published: February 2023

AbstractBackgroundAntimicrobial resistance (AMR) is propagated by widespread inappropriate use of antibiotics. In response, point-of-care interventions (POCIs) have been developed in primary care to preserve antibiotic effectiveness. Many of these POCIs are adopted based on their clinical value. However, assessment of their cost-effectiveness is crucial as well.ObjectivesTo summarize the evidence on cost-effectiveness of POCIs aimed at tackling inappropriate antibiotic prescriptions in primary care in middle- and high-income countries. We also evaluate the quality of the evidence with particular attention to how these economic evaluations faced the challenge of capturing the impact of these POCIs on AMR.MethodsSix scientific databases (MEDLINE, Embase, Web of Science, NHS EED, NHS HTA, the Cochrane Library) were searched for eligible articles published from 1999 to 2022. Their quality was appraised by means of the Drummond and CHEERS checklist.ResultsTwenty-nine articles met the selection criteria. Using their own (implicit) definitions of cost-effectiveness, evidence reported that point-of-care testing, scoring tools, electronic interventions, communication training, and multidimensional and educational interventions are more cost-effective than standard care. In contrast, studies found dipstick testing and audit-and-feedback interventions to be not cost-effective. Data synthesis took a narrative approach as eligible studies were not similar and/or reliable enough to pool their results through meta-analysis.ConclusionsMore high-quality evidence is needed to attain a thorough understanding of the cost-effectiveness of POCIs. Heterogeneity in terms of interventions and efficiency measures complicates comparing and generalizing results. Methodological recommendations are urgently needed to economically evaluate POCIs, focusing on how AMR should be accounted for.
Bacillus species: factories of plant protective volatile organic compounds
Authors: Poulaki Eirini G , Tjamos Sotiris E 
Published: February 2023

Abstract
Several studies have described the potential use of volatile organic compounds (VOCs) emitted by soil microorganisms, specifically of the genus Bacillus, as a sustainable solution for disease management in plants. The Bacillus species have been extensively studied as biocontrol agents (BCAs) due to their ability to inhibit pathogens, trigger induced systemic resistance (ISR) in plants, and enhance plant growth. The ability of the Bacillus species to produce long-lasting resting structures, such as endospores, makes them particularly appealing as BCAs. In recent years, there has been a growing body of research on the effects of Bacillus-emitted VOCs on plant pathogen growth and the triggering of ISR. This review aims to highlight recent advances in the understanding of the biological activities of Bacillus-emitted VOCs, identify new subjects for VOCs research, and stimulate interest in the academic and agri-business sectors for developing pre- and post-harvest application methods.
A framework to identify ethical concerns with ML-guided care workflows: a case study of mortality prediction to guide advance care planning
Authors: Cagliero Diana , Deuitch Natalie , Shah Nigam , Feudtner Chris , Char Danton 
Published: February 2023

AbstractObjectiveIdentifying ethical concerns with ML applications to healthcare (ML-HCA) before problems arise is now a stated goal of ML design oversight groups and regulatory agencies. Lack of accepted standard methodology for ethical analysis, however, presents challenges. In this case study, we evaluate use of a stakeholder “values-collision” approach to identify consequential ethical challenges associated with an ML-HCA for advanced care planning (ACP). Identification of ethical challenges could guide revision and improvement of the ML-HCA.Materials and MethodsWe conducted semistructured interviews of the designers, clinician-users, affiliated administrators, and patients, and inductive qualitative analysis of transcribed interviews using modified grounded theory.ResultsSeventeen stakeholders were interviewed. Five “values-collisions”—where stakeholders disagreed about decisions with ethical implications—were identified: (1) end-of-life workflow and how model output is introduced; (2) which stakeholders receive predictions; (3) benefit-harm trade-offs; (4) whether the ML design team has a fiduciary relationship to patients and clinicians; and, (5) how and if to protect early deployment research from external pressures, like news scrutiny, before research is completed.DiscussionFrom these findings, the ML design team prioritized: (1) alternative workflow implementation strategies; (2) clarification that prediction was only evaluated for ACP need, not other mortality-related ends; and (3) shielding research from scrutiny until endpoint driven studies were completed.ConclusionIn this case study, our ethical analysis of this ML-HCA for ACP was able to identify multiple sites of intrastakeholder disagreement that mark areas of ethical and value tension. These findings provided a useful initial ethical screening.
Preanalytical Factors Influencing the Stability of Ethanol in Antemortem Blood and Urine Samples
Authors: Olds Maria L , Kosecki Patrick A , Jones Alan W 
Published: February 2023

Abstract
The quantitative analysis of ethanol in blood and other biological specimens is a commonly requested service from forensic science and toxicology laboratories worldwide. The measured blood alcohol concentration (BAC) constitutes important evidence when alcohol-related crimes are investigated, such as drunken driving or drug-related sexual assault. This review article considers the importance of various preanalytical factors that might influence changes in the ethanol concentration in blood after collection and before analysis or reanalysis after various periods of storage. When blood samples were collected by venipuncture from living subjects in evacuated tubes containing sodium fluoride (NaF) preservative, there was no evidence that the BAC increased after collection. Most studies found that the BAC decreased after collection depending on storage conditions, such as time and temperature, and the amount of NaF preservative. After the storage of blood specimens in a refrigerator (4oC) for up to 1–4 weeks, the changes in the BAC were not analytically significant. After storage for up to 12 months at 4oC, under the same conditions, the BAC decreased on average by 0.01–0.02 g%. The loss of ethanol does not appear to depend on the type of evacuated tubes used (glass or plastic), nominal volume (5 mL or 10 mL) or the amount of NaF preservative. Urine alcohol concentrations were also stable after various periods of storage, although in cases of glycosuria and urinary tract and/or Candida infections, the addition of NaF (1% w/v) was essential to prevent post-sampling synthesis of ethanol.
Mutant NPM1 maintains RASGRP3 protein stability via interaction with MID1 to promote acute myeloid leukemia cell proliferation and autophagy
Authors: Wang Youping , Pan Chi , Zhang Xia , Zhao Aiqi , Dong Ying 
Published: February 2023

Abstract
Acute myeloid leukemia is a heterogeneous hematologic malignancy with high mortality in the world. NPM1 gene mutations are a frequent occurrence in acute myeloid leukemia, leading to abnormal autophagy, while the mechanism of NPM1 mutation-driven acute myeloid leukemia pathogenesis remains to be fully elucidated. GEO microarrays were used to screen for dysregulated autophagy-related genes in NPM1-mutant acute myeloid leukemia and analysis of RASGRP3 expression and prognosis. Next, we explored the potential molecular mechanisms relationship between RASGRP3 and NPM1 through utilizing immunoprecipitation, Western blot, and cycloheximide assay. Further, CCK8, EdU staining, immunofluorescence, and Western blot were performed to explore the effect of RASGRP3 on cell proliferation and apoptosis in NPM1-mutated acute myeloid leukemia. Finally, Western blot was used to study the mechanism of action of RASGRP3. RASGRP3 expression was upregulated in NPM1-mutated acute myeloid leukemia. Mislocalized NPM1-mA in the cytoplasm could bind to E3 ubiquitin-protein ligase MID1 to block degradation of the RASGRP3 protein. RASGRP3 could also activate the EGFR-STAT3 axis to promote proliferation and autophagy in acute myeloid leukemia. In conclusion, our results identified RASGRP3 as a proto-oncogene in NPM1-mutated acute myeloid leukemia. The RASGRP3-EGFR/STAT3 axis may be a promising therapeutic target for this unique leukemic subtype.
Using a novel CLEM system to localize lipid droplets and membranes in desiccated embryonic axis cells of soybean seeds
Authors: Khanam Salma , Funatsu Takayuki , Tanaka Koji , Kaneko Yasuko 
Published: February 2023

Abstract
Lipid droplets and membranes in radicle cells from desiccated embryonic axes of soybean (Glycine max) seeds were examined by a recently developed correlative light and electron microscopy system, which has been designed to facilitate the observation of identical locations using an upright reflected light microscope and compact SEM successively with minimum time lapse. Lipids are major components of membranes and are also stored in numerous lipid droplets lining plasma membranes in many seed cells. Fluorescently stained lipid droplets and membranes in the desiccated radicle cells were mainly located along the surface of shrunk protoplasm and around presumptive protein bodies, which will turn into vacuoles and increase their volume for radicle protrusion. Co-localization of lipid droplets and membranes suggests the presence of a membrane protection mechanism during desiccation and rehydration processes that ensures prompt elongation of radicle cells during germination.
Pharmaco-endoscopic therapy for weight regain post-gastric bypass: a case report
Authors: Jarrah Mohammad , Mokadem Mohamad 
Published: February 2023

AbstractAbout 1 in every 4 patients who undergo bariatric surgery regains significant amount of weight some time along their journey, posing it as a serious problem that needs to be addressed in a pandemic of obesity. Lifestyle modification, anti-obesity medications, and bariatric endoscopy are multiple therapeutic options that can be used to support any weight loss attempt. A 53-year-old woman with morbid obesity who responded adequately to gastric-bypass regained significant weight 8 years later. We initially approached her post-operative weight regain in a behavioral, pharmacologic non-invasive manner; however, she failed to appropriately respond to several anti-obesity medications. Upper endoscopy revealed a dilated gastric pouch and gastro-jejunal anastomosis (GJA) that was reduced using argon plasma coagulation (APC), but also with a modest response. We then added liraglutide to her APC endo-therapy sessions and subsequentially patient started losing significantly more weight. For selective post-bariatric surgery weight re-gainers, endoscopic and pharmaco-therapy combined may be needed for more effective results.
Equipping the Offline Population with Internet Access in an Online Panel: Does It Make a Difference?
Authors: Bach Ruben L , Cornesse Carina , Daikeler Jessica 
Published: February 2023

Abstract
Online panel surveys are often criticized for their inability to cover the offline population, potentially resulting in coverage error. Previous research has demonstrated that non-internet users in fact differ from online individuals on several sociodemographic characteristics. In attempts to reduce coverage error due to missing the offline population, several probability-based online panels equip offline households with an internet connection and a simple computer or tablet. However, the question remains whether the recruitment of offline individuals for an online panel leads to substantial changes in survey estimates. That is, it is unclear whether estimates derived from the survey data are affected by the differences between the groups of online and offline individuals. Against this background, we investigate how the inclusion of the previously offline population into the German Internet Panel affects various survey estimates such as voting behavior and social engagement. Overall, we find little evidence for the claim that equipping otherwise offline individuals with online access affects the estimates derived from previously online individuals only.
The Thousand-Pulsar-Array programme on MeerKAT – VIII. The subpulse modulation of 1198 pulsars
Authors: Song X , Weltevrede P , Szary A , Wright G , Keith M J , Basu A , Johnston S , Karastergiou A , Main R A , Oswald L S , Parthasarathy A , Posselt B , Bailes M , Buchner S , Hugo B , Serylak M 
Published: February 2023

ABSTRACT
We report on the subpulse modulation properties of 1198 pulsars using the Thousand-Pulsar-Array programme on MeerKAT. About 35 per cent of the analysed pulsars exhibit drifting subpulses that are more pronounced towards the death line, consistent with previous studies. We estimate that this common phenomenon is detectable in 60 per cent of the overall pulsar population if high-quality data were available for all. This large study reveals the evolution of drifting subpulses across the pulsar population in unprecedented detail. In particular, we find that the modulation period P3 follows a V-shaped evolution with respect to the characteristic age τc, such that the smallest P3 values, corresponding to the Nyquist period P3 ≃ 2, are found at τc ≃ 107.5 yr. The V-shaped evolution can be interpreted and reproduced if young pulsars possess aliased fast intrinsic P3, which monotonically increase, ultimately achieving a slow unaliased P3. Enhancement of irregularities in intrinsic subpulse modulation by aliasing in small-τc pulsars would explain their observed less well defined P3’s and weaker spectral features. Modelling these results as rotating subbeams, their circulation must slow down as the pulsar evolves. This is the opposite to that expected if circulation is driven by $\boldsymbol{E}\times \boldsymbol{B}$ drift. This can be resolved if the observed P3 periodicity is due to a beat between an $\boldsymbol{E}\times \boldsymbol{B}$ system and the pulsar period. As a by-product, we identified the correct periods and spin-down rates for 12 pulsars, for which harmonically related values were reported in the literature.