bluuebunny/crossref_metadata_2025_split huggingface dataset:
Dengue y su importancia semiológica: estudio de caso
Authors: Bezerra Lima Isabella , Neves da Silva Maricelly , Moraes Almeida Ana Caroline , Eredia Francisco Trevisan Isabella , Shuelton Romão de Araújo Yohana , Nogueira Andressa 
Published: March 2023

El dengue es causado por un virus perteneciente al género Flavivirus y transmitido por la picadura de un mosquito perteneciente al género Aedes, clasificado en 4 serotipos que presentan diferentes signos y síntomas, siendo cada vez más grave y difícil controlar la aparición de esta infección. Este trabajo aborda el caso clínico de una paciente que acudió a la Clínica 1 de la Universidad Central del Paraguay, con signos y síntomas de dengue del grupo B, evolución de 5 días, al principio se encontraba trombocitopénica y reactivo al antígeno NS1. Pasado los 6 días la paciente volvió a la clínica con nivel de plaquetas mejoradas y con el examen del antígeno NS1 positivo.
Palabras clave: Dengue, virus del dengue, caso clínico.

Abstract
Dengue is caused by a virus belonging to the genus Flavivirus and transmitted by the bite of a mosquito belonging to the genus Aedes, classified into 4 serotypes that present different signs and symptoms, being increasingly serious and difficult to control the appearance of this infection. This paper addresses the clinical case of a patient who attended Clinic 1 of the Central University of Paraguay, with signs and symptoms of group B dengue, evolution of 5 days, at first was thrombocytopenic and reactive to the NS1 antigen. After 6 days, the patient returned to the clinic with an improved platelet level and a positive NS1 antigen test.
Keywords: dengue, dengue virus, clinical report.
Enseñanza y aprendizaje del español en la escuela secundaria de Senegal: análisis de las creencias del profesorado
Authors: Ndour Seynabou 
Published: March 2023

This study reports on the problems and needs around the teaching of Spanish in Senegal. More specifically, it explores the complex belief system of secondary school teachers about secondary education processes. For this purpose, a qualitative methodology based on interviews with teachers, class observations and bibliographic analysis was used. In order to analyze the interviews, a codification process based on Grounded Theory was used, with the support of the ATLAS.ti software. The results of the study revealed the use of a traditional methodology for teaching Spanish, the lack of teacher knowledge on how to apply the communicative method, a teacher-centered approach, and the limited pedagogical training provided to teachers
Este trabajo centra su interés en los problemas y necesidades en torno a la enseñanza del español en Senegal. Más concretamente, explora el complejo sistema de creencias del profesorado de educación secundaria acerca de los procesos educativos. Para ello, se ha empleado una metodología cualitativa-interpretativa basada en entrevistas a los docentes, observaciones de clase y análisis bibliográfico. Para el análisis de entrevistas se ha realizado una codificación siguiendo la teoría fundamentada, con apoyo del software ATLAS.ti. Entre los resultados del estudio, destaca la observación del empleo de una metodología tradicional de enseñanza del español en el aula, la falta de conocimiento de los docentes sobre cómo aplicar el enfoque comunicativo, el rol de protagonista del docente frente a alumnos pasivos, y la limitada formación inicial y pedagógica proporcionada
“Mamá, me quiero ir de este colegio”. Un análisis de las experiencias de acoso escolar de estudiantes con diversidad funcional
Authors: Castro-Álvarez Rocío , Gómez-Marí Irene , Tárraga-Mínguez Raúl 
Published: March 2023

Bullying is a high-prevalence problem in the classrooms of our schools. Young people with functional diversity constitute a particularly vulnerable population when they are involved in such situations. This study analyses the harassment experiences of five young people with functional diversity. To this end, five semi-structured interviews and a focus group were conducted. Participants reported having experienced verbal, physical, social and sexual harassment. The treatment of these situations by educational institutions was ineffective and the solution adopted in all cases was a change in the type of schooling. These results highlight the need to carry out teacher training and sensitization actions in students to prevent and act on bullying situations, especially when students with functional diversity are involved.
El acoso escolar es un problema de elevada prevalencia en las aulas de nuestras escuelas. Los jóvenes con diversidad funcional constituyen una población especialmente vulnerable cuando se ve envuelta en este tipo de situaciones. En el presente estudio analizamos las experiencias de acoso de cinco jóvenes con diversidad funcional. Para ello, se han llevado a cabo cinco entrevistas semiestructuradas y un grupo de discusión. Los estudiantes relatan haber sufrido situaciones de acoso verbal, físico, social y sexual. El tratamiento de estas situaciones por parte de los centros educativos fue ineficaz y la solución adoptada en todos los casos fue un cambio en la modalidad de escolarización. Estos resultados resaltan la necesidad de llevar a cabo acciones de formación del profesorado y sensibilización en los estudiantes para prevenir y actuar ante situaciones de acoso, especialmente cuando se ven involucrados estudiantes con diversidad funcional.
Evaluation of antibody kinetics and durability in healthy individuals vaccinated with inactivated COVID-19 vaccine (CoronaVac): A cross-sectional and cohort study in Zhejiang, China
Authors: Zhang Hangjie , Hua Qianhui , Nani Xu Nani , Zhang Xinpei , Chen Bo , Ma Xijun , Hu Jie , Chen Zhongbing , Yu Pengfei , Lei Huijun , Wang Shenyu , Ding Linling , Fu Jian , Liao Yuting , Yang Juan , Jiang Jianmin , Lv Huakun 
Published: March 2023

Background:Although inactivated COVID-19 vaccines are proven to be safe and effective in the general population, the dynamic response and duration of antibodies after vaccination in the real world should be further assessed.Methods:We enrolled 1067 volunteers who had been vaccinated with one or two doses of CoronaVac in Zhejiang Province, China. Another 90 healthy adults without previous vaccinations were recruited and vaccinated with three doses of CoronaVac, 28 days and 6 months apart. Serum samples were collected from multiple timepoints and analyzed for specific IgM/IgG and neutralizing antibodies (NAbs) for immunogenicity evaluation. Antibody responses to the Delta and Omicron variants were measured by pseudovirus-based neutralization tests.Results:Our results revealed that binding antibody IgM peaked 14–28 days after one dose of CoronaVac, while IgG and NAbs peaked approximately 1 month after the second dose then declined slightly over time. Antibody responses had waned by month 6 after vaccination and became undetectable in the majority of individuals at 12 months. Levels of NAbs to live SARS-CoV-2 were correlated with anti-SARS-CoV-2 IgG and NAbs to pseudovirus, but not IgM. Homologous booster around 6 months after primary vaccination activated anamnestic immunity and raised NAbs 25.5-fold. The neutralized fraction subsequently rose to 36.0% for Delta (p=0.03) and 4.3% for Omicron (p=0.004), and the response rate for Omicron rose from 7.9% (7/89)–17.8% (16/90).Conclusions:Two doses of CoronaVac vaccine resulted in limited protection over a short duration. The inactivated vaccine booster can reverse the decrease of antibody levels to prime strain, but it does not elicit potent neutralization against Omicron; therefore, the optimization of booster procedures is vital.Funding:Key Research and Development Program of Zhejiang Province; Key Program of Health Commission of Zhejiang Province/ Science Foundation of National Health Commission; Major Program of Zhejiang Municipal Natural Science Foundation; Explorer Program of Zhejiang Municipal Natural Science Foundation.
Concordance of chest x-ray with chest CT by body mass index
Authors: Heston Thomas F. , Jiang John Y. 
Published: March 2023

Introduction
Patients with suspected thoracic pathology frequently get imaging with conventional radiography or chest x-rays (CXR) and computed tomography (CT). CXR include one or two planar views, compared to the three-dimensional images generated by chest CT. CXR imaging has the advantage of lower costs and lower radiation exposure at the expense of lower diagnostic accuracy, especially in patients with large body habitus.

Objectives
To determine whether CXR imaging could achieve acceptable diagnostic accuracy in patients with a low body mass index (BMI).

Methods
This retrospective study evaluated 50 patients with age of 63 ± 12 years old, 92% male, BMI 31.7 ± 7.9, presenting with acute, nontraumatic cardiopulmonary complaints who underwent CXR followed by CT within 1 day. Diagnostic accuracy was determined by comparing scan interpretation with the final clinical diagnosis of the referring clinician.

Results
CT results were significantly correlated with CXR results (r = 0.284, p = 0.046). Correcting for BMI did not improve this correlation (r = 0.285, p = 0.047). Correcting for BMI and age also did not improve the correlation (r = 0.283, p = 0.052), nor did correcting for BMI, age, and sex (r = 0.270, p = 0.067). Correcting for height alone slightly improved the correlation (r = 0.290, p = 0.043), as did correcting for weight alone (r = 0.288, p = 0.045). CT accuracy was 92% (SE = 0.039) vs. 60% for CXR (SE = 0.070, p < 0.01).

Conclusion
Accounting for patient body habitus as determined by either BMI, height, or weight did not improve the correlation between CXR accuracy and chest CT accuracy. CXR is significantly less accurate than CT even in patients with a low BMI.
Editorial
Authors: Mata Ana Maria Álvares Tavares da 
Published: March 2023

A Ciência no mundo pós-pandemia
Após os primeiros casos conhecidos na China em dezembro de 2019, o vírus SARS-Cov-2 alastrou rapidamente além fronteiras levando a Organiazação Mudial de Saude (OMS) a declarar em 30 de janeiro de 2020 o estado de “Emergência de Saúde Pública de Importância Internacional (ESPII)”, o mais alto nível de alerta, e em 11 de março de 2020, a COVID-19 foi caracterizada como uma pandemia pela OMS.
Pouco tempo após essa declaração, a maioria dos países iniciou períodos de lock-down total, situação inédita na história mundial. Vivi esse período conturbado em Portugal, onde moro e onde o Ministério da Saúde teve uma importância fundamental na comunicação com a população, emitindo comunicados diários com a evolução da situação e indicando os passos a seguir para manter a segurança de todos. Tudo isso com base nas informações dos especialistas. Como nunca, o conhecimento científico foi a única ferramenta útil para lidar com a realidade e enfrentar a pandemia. No caso de Portugal, um país com uma população envelhecida e, por isso, muito vulnerável, foi a ciência que permitiu que a percentagem de vítimas não tenha sido avassaladora.
O que aprendemos, o que sabemos agora no mundo pós-pandemia? Acredito que a pandemia tenha trazido para o centro da sociedade, de novo, a importância da ciência e de como ela funciona: quase sendo uma entidade orgânica viva, em constante adaptação e aperfeiçoamento que avança e traz esperança à humanidade. E que se quer para o futuro mais e melhor ciência! Quer-se ciência centrada na melhoria da qualidade de vida das populações e na preservação do planeta.
A disseminação da ciência é um passo fundamental do processo, e a Cadernos de Prospecção cumpre, com mais esta edição, a sua vocação de veículo transmissor de conhecimento. Nesta edição especial, “Covid-19: o mundo pós-pandemia” apresenta-se artigos de um total de 34 autores, pertencentes a 16 instituições Científicas, Tecnológicas e de Inovação pertencentes a 12 estados distintos do Brasil.
Boas leituras e viva a Ciência!!

Ana Maria Álvares Tavares da Mata
Professora Adjunta do Instituto Politécnico de Setúbal (ESTS-IPS), Portugal
Investigadora do CINEA, Centro de Investigação em Energia e Ambiente do IPS, Portugal
Investigadora do iBB, Instituto de Bioengenharia e Biociências, Instituto Superior Técnico, Universidade de Lisboa, Portugal
The Effect of Cryoprotectants and Storage Conditions on the Transfection Efficiency, Stability, and Safety of Lipid‐Based Nanoparticles for mRNA and DNA Delivery
Authors: Kafetzis Konstantinos N. , Papalamprou Natalia , McNulty Elisha , Thong Kai X. , Sato Yusuke , Mironov Aleksandr , Purohit Atul , Welsby Philip J. , Harashima Hideyoshi , Yu‐Wai‐Man Cynthia , Tagalakis Aristides D. 
Published: March 2023

AbstractLipid‐based nanoparticles have recently shown great promise, establishing themselves as the gold standard in delivering novel RNA therapeutics. However, research on the effects of storage on their efficacy, safety, and stability is still lacking. Herein, the impact of storage temperature on two types of lipid‐based nanocarriers, lipid nanoparticles (LNPs) and receptor‐targeted nanoparticles (RTNs), loaded with either DNA or messenger RNA (mRNA), is explored and the effects of different cryoprotectants on the stability and efficacy of the formulations are investigated. The medium‐term stability of the nanoparticles was evaluated by monitoring their physicochemical characteristics, entrapment and transfection efficiency, every two weeks over one month. It is demonstrated, that the use of cryoprotectants protects nanoparticles against loss of function and degradation in all storage conditions. Moreover, it is shown that the addition of sucrose enables all nanoparticles to remain stable and maintain their efficacy for up to a month when stored at −80 °C, regardless of cargo or type of nanoparticle. DNA‐loaded nanoparticles also remain stable in a wider variety of storage conditions than mRNA‐loaded ones. Importantly, these novel LNPs show increased GFP expression that can signify their future use in gene therapies, beyond the established role of LNPs in RNA therapeutics.
l‐Arginine‐Modified CoWO4/FeWO4 S‐Scheme Heterojunction Enhances Ferroptosis against Solid Tumor
Authors: Yang Zhuoran , Yang Chunyu , Yang Dan , Zhang Ye , Yang Qingzhu , Qu Fengyu , Guo Wei 
Published: March 2023

AbstractFerroptosis has recently attracted much attention as an anti‐tumor therapy. Evidence suggests that ferroptosis can induce oxidative stress and accumulation of lethal lipid peroxides in cancer cells, leading to cell damage. However, unsuitable pH, H2O2 levels, and high glutathione (GSH) expression in the tumor microenvironment hinder the development of ferroptosis‐mediated therapy. In this study, an l‐arginine (l‐arg)‐modified CoWO4/FeWO4 (CFW) S‐scheme heterojunction is strategically designed and constructed for ultrasound (US)‐triggered sonodynamic‐ and gas therapy‐induced ferroptosis. CFW not only has excellent Fenton‐catalytic activity, outstanding GSH consumption capacity, and excellent ability to overcome tumor hypoxia, but its S‐scheme heterostructure can also avoid the rapid combination of electron (e) and hole (h+) pairs, thereby enhancing the sonodynamic effects. As a precursor of nitric oxide (NO), l‐arg is modified on the surface of CFW (CFW@l‐arg) to achieve controlled NO release under US irradiation, thereby enhancing ferroptosis. In addition, poly(allylamine hydrochloride) is further modified on the surface of CFW@l‐arg to stabilize l‐arg and achieve controllable NO release. Both in vitro and in vivo results demonstrate that such a multifunctional therapeutic nanoplatform can achieve high therapeutic efficacy through sonodynamic and gas therapy‐enhanced ferroptosis. This designed oncotherapy nanoplatform provides new inspiration for ferroptosis‐mediated therapy.
Outersphere Approach to Increasing the Persistance of Oxygen‐Sensitive Europium(II)‐Containing Contrast Agents for Magnetic Resonance Imaging with Perfluorocarbon Nanoemulsions toward Imaging of Hypoxia
Authors: Lutter Jacob C. , Batchev Andrea L. , Ortiz Caitlyn J. , Sertage Alexander G. , Romero Jonathan , Subasinghe S. A. Amali S. , Pedersen Steen E. , Samee Md Abul Hassan , Pautler Robia G. , Allen Matthew J. 
Published: March 2023

AbstractRadiographic mapping of hypoxia is needed to study a wide range of diseases. Complexes of Eu(II) are a promising class of molecules to fit this need, but they are generally limited by their rapid oxidation rates in vivo. Here, a perfluorocarbon‐nanoemulsion perfused with N2, forms an interface with aqueous layers to hinder oxidation of a new perfluorocarbon‐soluble complex of Eu(II). Conversion of the perfluorocarbon solution of Eu(II) into nanoemulsions results in observable differences between reduced and oxidized forms by magnetic resonance imaging both in vitro and in vivo. Oxidation in vivo occurrs over a period of ≈30 min compared to <5 min for a comparable Eu(II)‐containing complex without nanoparticle interfaces. These results represent a critical step toward delivery of Eu(II)‐containing complexes in vivo for the study of hypoxia.
Tongue−Brain‐Transported ZnO Nanoparticles Induce Abnormal Taste Perception
Authors: Chen Aijie , Wang Ruolan , Kang Yiyuan , Liu Jia , Wu Junrong , Zhang Yanli , Zhang Yulin , Shao Longquan 
Published: March 2023

AbstractNanoparticles (NPs) can be transported to the brain, especially through nerves, because of their small size and high biological activity. Previous studies confirmed that zinc oxide (ZnO) NPs can enter the brain through the tongue−brain pathway, but it is unclear whether they will further affect synaptic transmission and brain perception. In this study, it is found that tongue−brain‐transported ZnO NPs can cause a decrease in taste sensitivity and taste aversion learning ability, indicating abnormal taste perception. Moreover, the release of miniature excitatory postsynaptic currents, the frequency of action potential release, and the expression of c‐fos are decreased, suggesting that the synaptic transmission is reduced. To further explore the mechanism, protein chip detection of inflammatory factors is carried out and it is found that neuroinflammation occurs. Importantly, it is found that neuroinflammation originated from neurons. The JAK‐STAT signaling pathway is activated, which inhibits the Neurexin1‐PSD95‐Neurologigin1 pathway and c‐fos expression. Blocking the activation of the JAK‐STAT pathway prevents neuroinflammation and the reduction in Neurexin1‐PSD95‐Neurologigin1. These results indicate that ZnO NPs can be transported by the tongue−brain pathway and lead to abnormal taste perception by neuroinflammation‐induced deficits in synaptic transmission. The study reveals the influence of ZnO NPs on neuronal function and provides a novel mechanism.
Fluorinated Li10GeP2S12 Enables Stable All‐Solid‐State Lithium Batteries
Authors: Jin Yuming , He Qinsheng , Liu Gaozhan , Gu Zhi , Wu Ming , Sun Tianyu , Zhang Zhihua , Huang Liangfeng , Yao Xiayin 
Published: March 2023

AbstractThe instability of Li10GeP2S12 toward moisture and that toward lithium metal are two challenges for the application in all‐solid‐state lithium batteries. In this work, Li10GeP2S12 is fluorinated to form a LiF‐coated core–shell solid electrolyte LiF@Li10GeP2S12. Density‐functional theory calculations confirm the hydrolysis mechanism of Li10GeP2S12 solid electrolyte, including H2O adsorption on Li atoms of Li10GeP2S12 and the subsequent PS43− dissociation affected by hydrogen bond. The hydrophobic LiF shell can reduce the adsorption site, thus resulting in superior moisture stability when exposing in 30% relative humidity air. Moreover, with LiF shell, Li10GeP2S12 shows one order lower electronic conductivity, which can significantly suppress lithium dendrite growth and reduce the side reaction between Li10GeP2S12 and lithium, realizing three times higher critical current density to 3 mA cm−2. The assembled LiNbO3@LiCoO2/LiF@Li10GeP2S12/Li battery exhibits an initial discharge capacity of 101.0 mAh g−1 with a capacity retention of 94.8% after 1000 cycles at 1 C.
Foldable RF Energy Harvesting System Based on Vertically Layered Metal Electrodes within a Single Sheet of Paper
Authors: Oh In Hyeok , Park Eiyong , Chang Suk Tai , Lim Sungjoon 
Published: March 2023

AbstractRadio frequency energy harvesting (RFEH) systems have emerged as a critical component for powering devices and replacing traditional batteries, with paper being one of the most promising substrates for use in flexible RFEH systems. However, previous paper‐based electronics with optimized porosity, surface roughness, and hygroscopicity still face limitations in terms of the development of integrated foldable RFEH systems within a single sheet of paper. In the present study, a novel wax‐printing control and water‐based solution process are used to realize an integrated foldable RFEH system within a single sheet of paper. The proposed paper‐based device includes vertically layered foldable metal electrodes, a via‐hole, and stable conductive patterns with a sheet resistance of less than 1 Ω sq−1. The proposed RFEH system exhibits an RF/DC conversion efficiency of 60% and an operating voltage of 2.1 V in 100 s at a distance of 50 mm and a transmitted power of 50 mW. The integrated RFEH system also demonstrates stable foldability, with RFEH performance maintained up to a folding angle of 150°. The single‐sheet paper‐based RFEH system thus has the potential for use in practical applications associated with the remote powering of wearable and Internet‐of‐Things devices and in paper electronics.
Improved Carrier Management via a Multifunctional Modifier for High‐Quality Low‐Bandgap Sn–Pb Perovskites and Efficient All‐Perovskite Tandem Solar Cells
Authors: Luo Jincheng , He Rui , Lai Huagui , Chen Cong , Zhu Jingwei , Xu Yuliang , Yao Fang , Ma Tianshu , Luo Yi , Yi Zongjin , Jiang Yiting , Gao Zhiyu , Wang Juncheng , Wang Wenwu , Huang Hao , Wang Ye , Ren Shengqiang , Lin Qianqian , Wang Changlei , Fu Fan , Zhao Dewei 
Published: March 2023

AbstractAll‐perovskite tandem solar cells (TSCs) hold great promise in terms of ultrahigh efficiency, low manufacturing cost, and flexibility, stepping forward to the next‐generation photovoltaics. However, their further development is hampered by the relatively low performance of low‐bandgap (LBG) tin (Sn)–lead (Pb) perovskite solar cells (PSCs). Improving the carrier management, including suppressing trap‐assisted non‐radiative recombination and promoting carrier transfer, is of great significance to enhance the performance of Sn–Pb PSCs. Herein, a carrier management strategy is reported for using cysteine hydrochloride (CysHCl) simultaneously as a bulky passivator and a surface anchoring agent for Sn–Pb perovskite. CysHCl processing effectively reduces trap density and suppresses non‐radiative recombination, enabling the growth of high‐quality Sn–Pb perovskite with greatly improved carrier diffusion length of >8 µm. Furthermore, the electron transfer at the perovskite/C60 interface is accelerated due to the formation of surface dipoles and favorable energy band bending. As a result, these advances enable the demonstration of champion efficiency of 22.15% for CysHCl‐processed LBG Sn–Pb PSCs with remarkable enhancement in both open‐circuit voltage and fill factor. When paired with a wide‐bandgap (WBG) perovskite subcell, a certified 25.7%‐efficient all‐perovskite monolithic tandem device is further demonstrated.