bluuebunny/crossref_metadata_2025_split huggingface dataset:
Microbial Degradation of Benzimidazole Fungicide Carbendazim by Bacillus velezensis HY-3479
Authors: Song Suyoung , Hwang Cher-Won 
Published: May 2023

Abstract
Carbendazim (Methyl benzimidazol-2-ylcarbamate: MBC) is a fungicide of the benzimidazole group which is widely used in the cultivation of pepper, ginseng, and many other crops. To remove the remnant carbendazim, many rhizobacteria are used as biodegradation agents. A bacterial strain of Bacillus velezensis HY-3479 was found to be capable of degrading MBC in M9 minimal medium added with 250 mg/L carbendazim. The strain had a significantly higher degradation rate compared to the control strain Bacillus subtilis KACC 15590 in HPLC analysis and HY-3479 had the best degradation rate of 76.99% at 48 hours. In gene expression analysis, upregulation of carbendazim degrading genes (mheI, hdx) was observed in the strain. HY-3479 was able to use MBC as the sole source of carbon and nitrogen but the addition of 12.5 mM NH4NO3 significantly raised the degradation rate. HPLC analysis showed that the degradation rate increased to 87.19% when added with NH4NO3. Relative gene expression of mheI and hdx also increased higher for samples with NH4NO3 supplement. The enzyme activity of carbendazim degrading enzyme and the 2-aminobenzimidazole degrading enzyme was found to be highly present in the HY-3479 strain. The biodegrading activity of strain HY-3479 may be developed as useful means for bioremediation and used as a potential microbial agent in sustainable agriculture.
A Novel Organic Semiconductor 4-phenylthiazol-2-yl-(phenylhydrazono) acetonitrile (PTPA) Thin Films: Synthesis, Optical and Electrical properties
Authors: Abuelwafa Amr Attia , Elnobi Sahar , Santos M. A. , Alsoghier H. M. 
Published: May 2023

Abstract
In this study, 4-phenylthiazol-2-yl-(phenylhydrazono) acetonitrile (PTPA) azo dye was synthesized and studied from optical and electrical point of view. The tautomerization phenomenon of the PTPA dye was clarified using one- dimensional (1D) and two- dimensional (2D) nuclear magnetic resonance (1HNMR and 13C NMR), absorbance (UV-Vis), emission, and infrared spectroscopy (FT-IR). X-ray diffraction (XRD) evaluations indicated that PTPA in powder and thin films crystallizes in a monoclinic system structure with nonostructural characteristics. Spectrophotometric measurements of absorbance A (λ), transmittance T (λ), and reflectance R (λ) at normal incidence light in the wavelength range 200–2500 nm were used to determine optical band gap, extinction coefficient, k, and refractive index, n. Also, non-linear optical parameters such as the third order non-linear susceptibility, χ(3) and nonlinear refractive index, n(2), of PTPA were revealed an awe-inspiring switching behavior, implying the possibility of using PTPA in optical switching systems. Finally, the electrical conductivity of the PTPA was raised with rising temperature, indicating that it is a typical organic semiconductor. Mott’s parameters were determined and discussed at low temperature. Thus, PTPA is a promising organic semiconductor with broad utility potential in organic electronics such as organic light-emitting diodes (OLEDs).
WITHDRAWN: Depression clinical detection model based on social media: a federated deep learning approach
Authors: Liu Yang 
Published: May 2023

Abstract
Depression can significantly impact people’s mental health, and recent research shows that social media can provide decision-making support for healthcare professionals and serve as supplementary information for understanding patients’ health status. Deep learning models are also able to assess an individual’s likelihood of experiencing depression. However, data availability on social media is often limited due to privacy concerns, even though deep learning models benefit from having more data to analyze. To address this issue, this study proposes a methodological framework system for clinical decision support that uses federated deep learning (FDL) to identify individuals experiencing depression and provide intervention decisions for clinicians. The proposed framework involves evaluation of datasets from three social media platforms, and the experimental results demonstrate that our method achieves state-of-the-art results. The study aims to provide a personalized clinical decision support system with evolvable features that can deliver precise solutions and assist healthcare professionals in medical diagnosis. The proposed framework that incorporates social media data and deep learning models can provide valuable insights into patients’ health status, support personalized treatment decisions, and adapt to changing healthcare needs.
Upfront primary tumor resection versus upfront systemic therapy for metastatic colorectal cancer: a systematic review and meta-analysis from randomized controlled trials and propensity score matched studies.
Authors: Lo Shih-Jung , Huang Shu-Huan , Lai I-Li , Chern Yih-Jong , Hsu Yu-Jen , Liao Chun-Kai , Cheng Ching-Chung , Tsai Wen-Sy , Hsieh Pao-Shiu , You Jeng-Fu 
Published: May 2023

Abstract
Purpose The standard initial treatment for metastatic colorectal cancer (mCRC) remains debated. This study investigated whether upfront primary tumor resection (PTR) or upfront systemic therapy (ST) provides better survival outcomes for patients with mCRC.Methods The PubMed, Embase, Cochrane Library, and ClinicalTrials.gov databases were searched for studies published at any time from January 1, 2004, to December 31, 2022. Randomized controlled trials (RCTs) and prospective or retrospective cohort studies (RCSs) utilizing propensity score matching (PSM) or inverse probability treatment weighting (IPTW) were included. We evaluated overall survival (OS) and short-term (60-day) mortality in these studies.Results After reviewing 3,626 articles, we identified 10 studies including a total of 38,947 patients. OS differed significantly between the upfront PTR and upfront ST arms (hazard ratio [HR] 0.62; 95% CI: 0.57–0.68; p < 0.001). However, a subgroup analysis identified no significant difference in OS in RCTs (HR 0.97; 95% CI: 0.7–1.34; p = 0.83), whereas significant difference in OS occurred between the treatment arms in RCSs with PSM or IPTW (HR 0.59; 95% CI: 0.54–0.64; p < 0.001). Short-term mortality was analyzed in three RCTs, and 60-day mortality differed significantly between the treatment arms (risk ratio [RR] 3.47; 95% CI: 1.21–9.96; p = 0.02).Conclusions In RCTs, upfront PTR for mCRC did not improve OS and enhanced the risk of 60-day mortality. By contrast, in RCSs, PSM or IPTW provided improved OS. Therefore, whether upfront PTR should be used for mCRC remains unclear. Further large RCTs are required.
A well-posed problem for the time fractional dual-phase-lag heat conduction
Authors: Sankeshwari Sagar , Kulkarni Vinayak 
Published: May 2023

Abstract

This is an attempt to reconstruct the theory of a well-posed problem for the dual-phase-lag heat conduction developed by Quintanilla in the context of time-fractional derivatives of order $\alpha \in (0,2] $. The heat conduction equation has been derived in the context of two temperature theory. The well-posedness of the problem has been proved by showing continuous dependence of the solution on the initial data and energy supply term. Moreover, the existence and uniqueness of the solution also have been proved subject to stability condition for the constitutive coefficients on the point spectrum. Due to the application of phase-lag $\tau$ = $\tau_{q}$ - $\tau_{T}$ satisfying $\tau$ $> 0$, thermal wave propagates with the finite speed. As a special case, an illustrative example has been discussed for heat transfer and thermal stress analysis of the solid heat conductor in the Cartesian domain. The analytical solutions have been achieved in the Laplace domain. The time domain inversions of the Laplace transforms have been numerically accomplished using the Gaver-Stehfest algorithm, satisfying Kuznetsov's convergence condition. The localized heat conduction $\alpha =0$, the classical thermoelasticity $\alpha =1$, the fractional thermoelasticity $\alpha =0.5$ and thermoelasticity without energy dissipation $\alpha =2$ have been discussed numerically and illustrated graphically.
Mathematics Subject Classification (2010) 26A33 · 35L35 · 44A10 · 65R10 · 74F05
Lipid metabolism disturbances in papillary thyroid cancer patients and the relationship with iodine nutrition status
Authors: Zhai Yuqian , Wang Xuebin , Luo Jie , Wang Xueqian , Liu Zhonghao , Wang Junrong , Liu Zhiyong , Mao Xionghui , Fan Lijun 
Published: May 2023

Abstract
Objective: Cancer patients are frequently accompanied by problems in lipid metabolism. Uncertainty exists as to whether changes in serum lipids occur in patients with papillary thyroid cancer (PTC) and their relationship with iodine nutrition remains obscure. The aim of this study was to explore lipid metabolism disturbances in PTC patients and their relationship with iodine nutrition status.
Methods: A total of 909 patients who were initially diagnosed with PTC and 183 patients who were initially diagnosed with benign thyroid nodules were enrolled in this study. The serum iodine concentration (SIC), the urine iodine concentration (UIC) and nine serum lipids indicators were measured. The generalized linear model (GLM) together with other statistical methods were used to determine whether there were differences in serum lipids between patients with PTC and those with benign thyroid nodules.
Results: After adjusting for baseline information, triglycerides (TG) levels in the control group (4.29±1.21) were significantly higher than in the cancer group (1.59±1.25). The rate of abnormal thyroid function was significantly lower in the patients with PTC than in the patients with benign nodules. In the PTC patients, different clinicopathological features had an impact on thyroid function, as reflected by a significant increase in FT3 in PTC with lymph node metastases, a significant increase in TSH, TGAb, and TPOAb, and a significant decrease in FT4 in PTC with AITD. Correlation analysis revealed weak to moderate correlations between iodine nutritional status, thyroid function, and serum lipids. In benign thyroid nodule patients, LDL-C and ApoB values in patients with benign thyroid nodules were significantly higher in the high SIC group than in the adequate and deficient groups. In PTC patients. ApoE levels in the low UIC group were significantly higher than in the middle and high UIC groups. Mediating effects were used to analyze the effect of iodine nutrition on the serum lipids, it showed that the total and direct effects of iodine nutritional status on serum lipids were significant, and the mediating effect of thyroid function was not significant.
Conclusion: TG levels in the control group were significantly higher than in the PTC group. Iodine nutritional status influences lipids, and an excess or deficient iodine nutrition increases the risk of dyslipidemia in patients with thyroid nodule. Iodine nutritional status had a direct effect on serum lipids.
Roseobacter Weihaiensis soli sp. nov., an actinobacterium isolated from limpet collected from Golden Beach in Weihai, China
Authors: Li Yu-Rui , Zhang Ming-Jing , Yi Yan-Jun , Wang Xiao-Chen , San Dong-Mei , Zhou Yan-Xia 
Published: May 2023

Abstract
Strain H9T belongs to the genus Roseobacter, with positive gram stain, catalase negative, oxidase negative, pink purple, rod-shaped, amastigote, isolated from lempet collected from the Golden Beach, Weihai, Shandong Province, China. The new isolates was able to grow at 4-42 °C (optimum, 25 °C), at pH 6.5-9.0 (optimum, pH 7.0), and with 0.0-11.0% (w/v) NaCl (optimum, 3.0-5.0%). By phylogenetic analysis of the 16S rRNA gene sequence, the novel marine isolate belongs to the genus Roseobacter, the related strains Roseobacter cerasinus AI77T and Roseobacter ponti MM-7T, and the pairwise sequence showed 98.5% similarity. The summary characteristics of the main fatty acids was summed feature 8 (C18:1ω7c and/or C18:1ω6c; 81.08%). The only respiratory quinoid is Q-10. The polar lipids consisted of phosphatidylglycerol, phosphatidylcholine, phosphatidylglycerol, an unknown lipid, and a small amount of an unknown phospholipid. Based on the draft genome sequence map, the strain H9T genome length was 5,351,685 bp, and the DNA G + C content was 59.8%. The average amino acid identity (AAI) of the genome of strain H9T and closely related strains, and the average nucleotide identity (ANI) and digital DNA hybridization (dDDH) values were 63.4-76.8%, 76.2-78.8%, and 13.4-19.7%, respectively. Thus, the combination of phenotypic, chemical taxonomic, and phylogenetic data suggests that strain H9T is considered a new species in the genus Roseobacter sp. The strain H9T (= KCTC 82507T = MCCC 1K04354T) was presented in November.
Spatial genomic, biochemical, and cellular mechanisms drive meningioma heterogeneity and evolution
Authors: Lucas Calixto-Hope , Mirchia Kanish , Seo Kyounghee , Najem Hinda , Chen William , Zakimi Naomi , Choudhury Abrar , Liu S. John , Phillips Joanna , Magill Stephen , Horbinski Craig , Solomon David , Perry Arie , Vasudevan Harish , Heimberger Amy , Raleigh David 
Published: May 2023

Abstract

Intratumor heterogeneity underlies cancer evolution and treatment resistance1–5, but targetable mechanisms driving intratumor heterogeneity are poorly understood. Meningiomas are the most common primary intracranial tumors and are resistant to all current medical therapies6,7. High-grade meningiomas cause significant neurological morbidity and mortality and are distinguished from low-grade meningiomas by increased intratumor heterogeneity arising from clonal evolution and divergence8. Here we integrate spatial transcriptomic and spatial protein profiling approaches across high-grade meningiomas to identify genomic, biochemical, and cellular mechanisms linking intratumor heterogeneity to the molecular, temporal, and spatial evolution of cancer. We show divergent intratumor gene and protein expression programs distinguish high-grade meningiomas that are otherwise grouped together by current clinical classification systems. Analyses of matched pairs of primary and recurrent meningiomas reveal spatial expansion of sub-clonal copy number variants underlies treatment resistance. Multiplexed sequential immunofluorescence (seqIF) and spatial deconvolution of meningioma single-cell RNA sequencing show decreased immune infiltration, decreased MAPK signaling, increased PI3K-AKT signaling, and increased cell proliferation drive meningioma recurrence. To translate these findings to clinical practice, we use epigenetic editing and lineage tracing approaches in meningioma organoid models to identify new molecular therapy combinations that target intratumor heterogeneity and block tumor growth. Our results establish a foundation for personalized medical therapy to treat patients with high-grade meningiomas and provide a framework for understanding therapeutic vulnerabilities driving intratumor heterogeneity and tumor evolution.
Gene expression imputation provides clinical and biological insights into treatment-resistant schizophrenia polygenic risk
Authors: Prohens  , Rodriguez Natalia , Segura Alex , Martinez-Pinteño Albert , Olivares-Berjaga David , Martínez Irene , González Aitor , Mezquida  , Gasso Patricia , Parellada Mara , Cuesta Manuel , Bernardo Miquel , Mas Sergi 
Published: May 2023

Abstract
Treatment-resistant schizophrenia (TRS) is a complex phenotype with important implications in the clinical course of a disease that is both underdiagnosed and poorly treated. The identification of predictors and biomarkers for the early detection and treatment of TRS is a critical step. Large genome-wide association studies (GWAS) have revealed the polygenic nature of TRS. Gene expression imputation allowed the translation of genetic variants into regulatory mechanisms that can be used to assess its association with clinical features of TRS in a cohort with a first episode of psychosis (FEP). We used S-PrediXcan to perform transcriptome imputation in the largest GWAS of TRS to find genetically regulated gene expression (GReX) associated with TRS using GTEx brain tissues. Then, for each tissue. we constructed a GReX risk score (GReX-PS) of the identified genes in a sample of genotyped FEP patients to test the association of the GReX-PS with clinical phenotypes, including clinical symptomatology, global functioning and cognitive performance. Our analysis provides evidence that the polygenic basis of TRS includes genetic variants that modulate the expression of certain genes in certain brain areas (substantia nigra, hippocampus, amygdala and frontal cortex), which at the same time are related to clinical features in FEP patients, mainly persistence of negative symptoms and cognitive alterations in sustained attention, which have also been suggested as clinical predictors of TRS. Our results provide a clinical explanation of the polygenic architecture of TRS and give more insight into the biological mechanisms underlying TRS.
WITHDRAWN: Clinical Evaluation of Caries Severity, Salivary and Plaque pH of Children with Molar Incisor Hypomineralization: A Cross-sectional Study
Authors: Sukesh Sonali , jain mansi , Sogi H.P. Suma , Gambhir Apurva , Kashalkar Bhairavi , Benson Tanya Liz 
Published: May 2023

Abstract

Introduction: Molar-incisor-hypomineralization has become a major thread of recent studies due to its role as a risk factor for dental caries but studies regarding the aetiology are limited. The knowledge of associated factors is desirable for the better understanding detecting and management of MIH in affected patients.
Aim: To determine the salivary and plaque pH in 8–15-year-old children with and without MIH and to correlate it with caries severity in children with MIH.
Materials and Method: 209 children aged 8-15 years were selected by stratified sampling. Hypo mineralised teeth and caries were assessed using EAPD criteria and ICDAS II criteria respectively. Salivary and plaque samples were collected and pH was determined.
Results: Statistically significant difference in salivary pH was observed between the groups A1, A2, B, C and A,B,C( P = 0.001*). On pair wise comparison, significant differences were observed in all pairs of groups except group B and group A. Statistically significant difference in plaque pH was seen between the groups A2, C (P= 0.001*).On pair wise comparison, significant differences were seen in all pairs of groups except group A1,B and A,B. The ICDAS II criteria scores were significantly higher in MIH group.
Conclusion: Statistically significant increase in caries severity and a fall in the mean salivary and plaque pH associated with Post-eruptive- breakdown in the MIH affected population was observed.
Practical implications: Hypo mineralized lesions disguised by caries demanding, thus it’s important to take other factors such as salivary properties into consideration for a better understanding of the defect
The Association of Radial Head Enlargement with Osteochondritis Dissecans of the Humeral Capitellum
Authors: Urita Atsushi , Tokuhiro Taiki , Kawamura Daisuke , Inoue Nozomu , Taneichi Hiroshi , Iwasaki Norimasa 
Published: May 2023

Abstract

Radial head enlargement is a secondary change when capitellar osteochondritis dissecans (OCD) lesions progress. This study was performed to clarify geometric differences between the radial head of the OCD side and the contralateral side in patients with capitellar OCD, and to investigate the correlation between radial head enlargement and elbow range of motion. Eighteen patients with capitellar OCD were underwent computed tomography (CT) images of bilateral elbow joints. Five patients underwent conservative treatment (Nonoperative group) and 13 patients underwent operative treatment (Operative group). Three-dimensional (3D) CT models of the proximal radius were created and exported into polygon and point-cloud models. The radial head geometry of the OCD side was compared with that of the mirror-imaged model of the contralateral side by using a 3D–3D registration technique. The surface-to-surface least distance was calculated. Mean least distance was 1.08 mm (SD 0.55 mm.) The radial head in the Operative group was significantly larger than that in the Nonoperative group (p= 0.02). Elbow flexion correlated negatively with radial head enlargement (P= 0.02 and r = 0.552). Our results clarified that the radial head enlargement increases with disease progression and correlated negatively with elbow flexion in capitellar OCD.
Mechanism-based Suppression of Cancer by Targeting DNA-Replicating
Enzymes
Authors: Arya Preeti , Malhotra Hitesh , Chaudhary Benu , Sarwara Amrit , Goyal Rajat , Wan Chunpeng , Mishra Dinesh Kumar , Gautam Rupesh Kumar 
Published: May 2023

Abstract:
The human genetic structure undergoes continuous wear and tear process due to the mere
presence of extrinsic as well as intrinsic factors. In normal physiological cells, DNA damage initiates
various checkpoints that may activate the repair system or induce apoptosis that helps maintain cellular
integrity. While in cancerous cells, due to alterations in signaling pathways and defective checkpoints,
there exists a marked deviation of error-free DNA repairing/synthesis. Currently, cancer therapy
targeting the DNA damage response shows significant therapeutic potential by tailoring the therapy
from non-specific to tumor-specific activity. Recently, numerous drugs that target the DNA replicating
enzymes have been approved or some are under clinical trial. Drugs like PARP and PARG inhibitors
showed sweeping effects against cancer cells. This review highlights the mechanistic study of
different drug categories that target DNA replication and thus depicts the futuristic approach of targeted
therapy.
Antiproliferative Activity and Mechanisms of Action of Plant-derived Flavonoids on Breast Cancer
Authors: Criollo-Mendoza Marilyn S. , Heredia J. Basilio , Vazquez-Olivo Gabriela , Avilés-Gaxiola Sara , Gutiérrez-Grijalva Erick P. , Garcia-Carrasco Melissa 
Published: May 2023

Abstract:Breast cancer is one of the main global diseases with a high mortality rate that mainly af-fects the female population. Despite the important advances that have been made concerning the treatments for this disease, research on less invasive therapies that generate fewer side effects for patients continues to develop. Consequently, researchers have turned their attention to using natural compounds (such as flavonoids) involved in molecular processes implicated in this type of cancer and are studying how these processes can be exploited to develop possible chemotherapies. This re-view offers a general description of studies on the antiproliferative activity of flavonoids obtained from natural sources for breast cancer treatment and their mechanism of action related to their struc-tural characteristics. Reports were retrieved from electronic databases, such as Web of Science and Scopus using the following keywords: breast cancer, antiproliferative, flavonoids, and structure-activity. Articles published between 2015-2022 related to the topics mentioned above were selected, focusing on the flavonoids apigenin, luteolin, quercetin, and naringenin, as they are the ones with the highest activity and relevance according to the literature found.