bluuebunny/crossref_metadata_2025_split huggingface dataset:
The potential of colchicine for lowering the risk of cardiovascular events in type 1 diabetes
Authors: Johansen Nicklas Järvelä , Knop Filip Krag 
Published: January 2023

Abstract
In type 1 diabetes, average life expectancy is reduced by ˃10 years as compared with outside of diabetes. Residual cardiovascular risk defines high cardiovascular event rate despite modern, guideline-recommended standard of care of established risk factors like hypertension, dyslipidaemia, and glycaemic control, and it adds importantly to these lost years of life in type 1 diabetes due to atherosclerotic cardiovascular diseases like myocardial infarction and ischaemic stroke. With a growing understanding of inflammation as an important driver of atherosclerotic cardiovascular disease, residual inflammatory risk is a novel and common risk factor and a promising target for lowering residual cardiovascular risk in type 1 diabetes. Interestingly, the inexpensive anti-inflammatory agent colchicine reduced the risk of major adverse cardiovascular events by 25% in cardiovascular outcome trials in the secondary prevention of atherosclerotic cardiovascular disease. Here, we summarize the role of inflammation as a driver of atherosclerosis and review current evidence linking inflammation and atherosclerotic cardiovascular disease in type 1 diabetes. Also, we provide an overview of the evidence base for targeting residual inflammatory risk with colchicine for lowering residual cardiovascular risk in type 1 diabetes.
Targeting endothelial tight junctions to predict and protect thoracic aortic aneurysm and dissection
Authors: Yang Xueyuan , Xu Chen , Yao Fang , Ding Qianhui , Liu Hao , Luo Congcong , Wang Daidai , Huang Jiaqi , Li Zhiqing , Shen Yicong , Yang Weijie , Li Zhuofan , Yu Fang , Fu Yi , Wang Li , Ma Qingbian , Zhu Junming , Xu Fujian , Cong Xin , Kong Wei 
Published: January 2023

AbstractAimsWhether changes in endothelial tight junctions (TJs) lead to the formation of thoracic aortic aneurysm and dissection (TAAD) and serve as an early indicator and therapeutic target remains elusive.Methods and resultsSingle-cell RNA sequencing analysis showed aberrant endothelial TJ expressions in the thoracic aortas of patients with TAAD. In a β-aminopropionitrile (BAPN)-induced TAAD mouse model, endothelial TJ function was disrupted in the thoracic aortas at an early stage (5 and 10 days) as observed by a vascular permeability assay, while the intercellular distribution of crucial TJ components was significantly decreased by en face staining. For the non-invasive detection of endothelial TJ function, two dextrans of molecular weights 4 and 70 kDa were conjugated with the magnetic resonance imaging (MRI) contrast agent Gd-DOTA to synthesize FITC-dextran-DOTA-Gd and rhodamine B-dextran-DOTA-Gd. MRI images showed that both probes accumulated in the thoracic aortas of the BAPN-fed mice. Particularly, the mice with increased accumulated signals from 5 to 10 days developed TAAD at 14 days, whereas the mice with similar signals between the two time points did not. Furthermore, the protease-activated receptor 2 inhibitor AT-1001, which seals TJs, alleviated the BAPN-induced impairment of endothelial TJ function and expression and subsequently reduced TAAD incidence. Notably, endothelial-targeted ZO-1 conditional knockout increased TAAD incidence. Mechanistically, vascular inflammation and edema were observed in the thoracic aortas of the BAPN-fed mice, whereas these phenomena were attenuated by AT-1001.ConclusionThe disruption of endothelial TJ function is an early event prior to TAAD formation, herein serving as a potential indicator and a promising target for TAAD.
Unipolar voltage mapping in right ventricular cardiomyopathy: pitfalls, solutions and advantages
Authors: Hoogendoorn Jarieke C , Venlet Jeroen , de Riva Marta , Wijnmaalen Adrianus P , Piers Sebastiaan R D , Zeppenfeld Katja 
Published: January 2023

Abstract

Aims
Endocardial unipolar and bipolar voltage mapping (UVM/BVM) of the right ventricle (RV) are used for transmural substrate delineation. However, far-field electrograms (EGMs) and EGM changes due to injury current may influence automatically generated UVM. Epicardial BVM is considered less accurate due to the impact of fat thickness (FT). Data on epicardial UVM are sparse. The aim of the study is two-fold: to assess the influence of the manually corrected window-of-interest on UVM and the potential role of epicardial UVM in RV cardiomyopathies.

Methods and results
Consecutive patients who underwent endo-epicardial RV mapping with computed-tomography (CT) integration were included. Mapping points were superimposed on short-axis CT slices and correlated with local FT. All points were manually re-analysed and the window-of-interest was adjusted to correct for false high unipolar voltage (UV). For opposite endo-epicardial point-pairs, endo-epicardial bipolar voltage (BV) and UV were correlated for different FT categories. A total of 3791 point-pairs of 33 patients were analysed. In 69% of endocardial points and 63% of epicardial points, the window-of-interest needed to be adjusted due to the inclusion of far-field EGMs, injury current components, or RV-pacing artifacts. The Pearson correlation between corrected endo-epicardial BV and UV was lower for point-pairs with greater FT; however, this correlation was much stronger and less influenced by fat for UV.

Conclusion
At the majority of mapping sites, the window-of-interest needs to be manually adjusted for correct UVM. Unadjusted UVM underestimates low UV regions. Unipolar voltage seems to be less influenced by epicardial fat, suggesting a promising role for UVM in epicardial substrate delineation.
Syncope in ICD recipients: a single centre experience
Authors: Khan Parisha , Selvarajah Karshana , Gohel Sheena , Sidhu Baldeep S , Cannatà Antonio , Bromage Daniel I , McDonagh Theresa , Murgatroyd Francis , Scott Paul A 
Published: January 2023

AbstractAimsThere is little evidence of the impact of syncope in implantable cardioverter-defibrillator (ICD) patients in routine community hospital care. This single-centre retrospective study sought to evaluate the incidence and prognostic significance of syncope in consecutive ICD patients.Methods and resultsData were collected on consecutive patients undergoing first ICD implantation between January 2009 and December 2019. The primary endpoints were the first occurrence of all-cause syncope, all-cause mortality, and all-cause hospitalization. Multivariate Cox proportional hazard models were used to identify risk factors associated with syncope and to analyse the subsequent risk of mortality and hospitalization. 1003 patients (58% primary prevention) were included in the final analysis. During a mean follow-up of 1519 ± 1055 days, 106 (10.6%) experienced syncope, 304 died (30.3%), and 477 (47.5%) were hospitalized for any cause. In an analysis adjusted for baseline variables, the first occurrence of syncope was associated with a significantly increased risk of mortality (HR 2.82, P &lt; 0.001) and the first occurrence of hospitalization (HR 2.46, P = 0.002).ConclusionSyncope in ICD recipients is common and associated with a poor prognosis irrespective of baseline variables and ICD programming. The occurrence of syncope is associated with a significant increase in the risk of mortality and hospitalization.
pCEC-red: a new vector for easier and faster CRISPR-Cas9 genome editing in Saccharomyces cerevisiae
Authors: Maestroni Letizia , Butti Pietro , Senatore Vittorio Giorgio , Branduardi Paola 
Published: January 2023

Abstract
CRISPR-Cas9 technology is widely used for precise and specific editing of Saccharomyces cerevisiae genome to obtain marker-free engineered hosts. Targeted double-strand breaks are controlled by a guide RNA (gRNA), a chimeric RNA containing a structural segment for Cas9 binding and a 20-mer guide sequence that hybridises to the genomic DNA target. Introducing the 20-mer guide sequence into gRNA expression vectors often requires complex, time-consuming, and/or expensive cloning procedures. We present a new plasmid for CRISPR-Cas9 genome editing in S. cerevisiae, pCEC-red. This tool allows to (i) transform yeast with both Cas9 and gRNA expression cassettes in a single plasmid and (ii) insert the 20-mer sequence in the plasmid with high efficiency, thanks to Golden Gate Assembly and (iii) a red chromoprotein-based screening to speed up the selection of correct plasmids. We tested genome-editing efficiency of pCEC-red by targeting the ADE2 gene. We chose three different 20-mer targets and designed two types of repair fragments to test pCEC-red for precision editing and for large DNA region replacement procedures. We obtained high efficiencies (∼90%) for both engineering procedures, suggesting that the pCEC system can be used for fast and reliable marker-free genome editing.
Risk of occupational infection in forensic workers: a review
Authors: Dufayet Laurène , Langrand Jerome , Ludes Bertrand 
Published: January 2023

Abstract
The occupational risk of infection in forensic workers is a cause for concern, furthermore in the current context of the coronavirus disease-19 (COVID-19) pandemic. In order to characterize this risk, we performed an extended review of the literature on occupational infections occurring in forensic workers. Seventeen articles were included. Direct contamination by aerosolization was the main mode of transmission reported, with 17 cases of tuberculosis. Indirect contamination was described as the mode of transmission in 10 cases (five cases of blastomycosis, two cases of tuberculosis, two Streptococcus pyogenes, and one case of human immunodeficiency virus). In all the other included cases, the mode of transmission was unknown. For two of them, the information provided was sufficient to link them to occupational exposure (one case of toxoplasmosis, one case of tuberculosis). For the remaining 10 cases, the link was uncertain (six cases of tuberculosis, three of hepatitis B, and one of COVID-19). Even if there is probably significant under-declaration, the number of infections linked to an occupational risk in forensic workers is not alarming, thanks to effective preventive measures.
Joint inversion of GNSS and GRACE/GFO data for terrestrial water storage changes in the Yangtze River Basin
Authors: Li Xianpao , Zhong Bo , Li Jiancheng , Liu Renli 
Published: January 2023

SUMMARYSatellite geodetic technologies, such as the Global Navigation Satellite System (GNSS), Gravity Recovery and Climate Experiment (GRACE) and GRACE Follow-On (GFO), have complementary advantages in inferring terrestrial water storage (TWS) changes at regional and basin scales. We introduced a joint inversion strategy to infer TWS changes using GNSS- and GRACE/GFO-derived vertical displacements based on Green's function theory in the Yangtze River Basin (YRB) from January 2011 to December 2020. Additionally, we investigated the performance of variance component estimation (VCE) and Akaike's Bayesian Information Criterion (ABIC) to determine the optimal relative weights of different observation data. The performance of our joint inversion strategy was verified through a closed-loop simulation and multi-source hydrometeorological data [i.e. the time derivative of TWS changes (${\rm d}S/{\rm d}t$) from precipitation (P), evapotranspiration (ET) and run-off (R) based on the water balance equation, called P-ET-R]. The closed-loop simulation shows that the TWS changes from joint inversion have better consistencies with the synthetic signals than those of GNSS- and GRACE-only estimates, and the corresponding root mean square error (RMSE) decreased 1.43−6.28 mm and correlation coefficient (CC) increased 3−10 per cent. The ABIC was more suitable for the joint inversion of measured GRACE/GFO and GNSS data for TWS changes in the YRB. Analysis from the measured data shows that the spatial patterns and seasonal characteristics in TWS changes derived from GNSS, GRACE/GFO and their joint inversion are in good agreement in the YRB. The contribution of GNSS observations to the joint inversion in the upstream of the YRB is greater than that of GRACE/GFO due to the relatively densely distributed GNSS stations, but the opposite is true in the downstream. Furthermore, the joint inversion results have better agreements with P and P-ET-R compared to GNSS- and GRACE/GFO-only estimates in the upstream, and the corresponding CCs increased 5−7 per cent (for P) and 2−5 per cent (for P-ET-R), respectively, which further demonstrates the effectiveness of our joint inversion strategy. Our estimation strategy provides a new insight for joint inversion of GNSS and GRACE/GFO data to obtain more reliable TWS changes.
Multiple streams approach to tobacco control policymaking in the capital city of the world’s largest tobacco consuming country
Authors: Zheng Yunting , Shi Yuhui , Ji Ying , Chang Chun 
Published: January 2023

Abstract
The aim of this study was to analyse the policymaking process of Beijing tobacco control regulations based on a multiple streams framework to provide a reference for other cities, at the national level and for the international community to promote the development of tobacco control policy. Twenty-one documents related to tobacco control in Beijing were collected, nine informants were interviewed and the interview data were analysed by a thematic framework method. It was found that indicators, feedback and a focus event in the problem stream drew the attention of policymakers and the society for tobacco control. In 2011, Ying Songnian, a representative of the Beijing Municipal People’s Congress, put forward tobacco control legislation, which was just in time for the legislative reform of the Congress. The proposal was studied by the Congress, and a strategy of ‘social co-governance’ was founded. In the political stream, the government actively promoted tobacco control and social organizations extensively participated in it. In 2013, the General Office of the Central Committee of the Chinese Communist Party and the General Office of the State Council issued a notice on matters related to leading cadres taking the lead in banning smoking in public places, which opened a policy window for decision-makers who were hesitatant. The issue of tobacco control was successfully put on the policy agenda and contributed to the introduction of the Beijing Municipal Regulations on Smoking Control. Development of the factors in problem stream, policy stream and political stream promoted the setting up of the tobacco control policy agenda in Beijing. It is suggested that more cities should learn from the experience of Beijing, seize the opportunity of the ideological change of the ruling party, actively identify the problems, mobilize and advocate for representatives and introduce the concept of ‘social co-governance’ to promote tobacco control legislation.
Impact of Baseline Disease Activity and Trial Duration on Efficacy of Biologics in Active Crohn’s Disease: Meta-analysis
Authors: Barberio Brigida , Gracie David J , Black Christopher J , Ford Alexander C 
Published: January 2023

Abstract

Background
Timings of assessment of efficacy and criteria used to define Crohn’s disease (CD) activity at baseline may affect therapeutic gain of active drug over placebo in induction of remission trials in CD, but these issues have not been assessed systematically. We examined these issues in a meta-analysis.

Methods
We searched the literature to June 2022 for randomized controlled trials of biologics vs placebo in active CD. We extracted clinical remission and response rates according to criteria used to define CD activity and time point of assessment, pooling them in a meta-analysis for all patients according to previous biologic exposure. We calculated the number needed to treat (NNT), with a 95% confidence interval (CI) to assess therapeutic gain of active drug over placebo according to these characteristics of trial design.

Results
We identified 20 induction of remission trials (6754 patients). Rates of clinical remission were highest (42.6% with active drug vs 21.0% with placebo) and NNT lowest (5; 95% CI, 3-7.5) in trials using clinical and endoscopic activity to define active CD. Rates of remission were lower (26.5% with active drug, vs 18.6% with placebo) and NNT highest (12; 95% CI, 6-61) in trials using clinical activity alone. Results were similar according to previous biologic exposure. Time point of assessment seemed to have less of an effect, although the NNT was lowest in trials assessing remission rates at 9 to 12 weeks (NNT = 5.5; 95% CI, 4-8). Again, results were similar according to previous biologic exposure.

Conclusions
Both the criteria used to define CD activity at study entry and the time point used to confirm efficacy may be important in maximizing therapeutic gain of active drug over placebo.
Appropriate Preconception Corticosteroid-Free Remission Period in Pregnant Women With Ulcerative Colitis
Authors: Ikeda Aya , Kunisaki Reiko , Aoki Shigeru , Yaguchi Katsuki , Madarame Akira , Nishio Masafumi , Ogashiwa Tsuyoshi , Nakamori Yoshinori , Kimura Hideaki , Suzuki Ryoichi , Saigusa Yusuke , Maeda Shin 
Published: January 2023

Abstract

Background
In women with inflammatory bowel disease, at least 3 months of preconception corticosteroid-free remission (CFREM) is recommended by experts in current consensus statements. However, data are lacking on the appropriate preconception remission period. We investigated the appropriate preconception CFREM period in women with ulcerative colitis to reduce maternal disease activity and adverse pregnancy outcomes (ie, preterm birth, low birth weight, and small for gestational age).

Methods
We retrospectively examined 141 pregnancies in women with ulcerative colitis at 2 institutions. We categorized the patients into 3 subgroups by their preconception CFREM period (≥3 months, &gt;0 to &lt;3 months, and non-CFREM). We also investigated disease activity during pregnancy and postpartum and adverse pregnancy outcomes in each group.

Results
During pregnancy, the rate of active disease was significantly lower in the ≥3 months and &gt;0 to &lt;3 months CFREM groups compared with that in the non-CFREM group (P &lt; .001 and P = .0257, respectively). Postpartum, the rate of active disease was significantly lower in the ≥3 months CFREM group compared with that in the non-CFREM group (P = .0087). The preconception CFREM period of ≥3 months was an independent inhibitory factor for active disease during pregnancy and postpartum (adjusted odds ratio, 0.15; P = .0035; and adjusted odds ratio, 0.33; P = .027, respectively). Adverse pregnancy outcomes were less common in the &gt;3 months CFREM group compared with those in the other groups, but this difference was not significant.

Conclusions
A preconception CFREM period of more than 3 months may be appropriate for better maternal and adverse pregnancy outcomes, as recommended in consensus statements.
Green innovation strategies and firms’ internationalization
Authors: Martínez-Ros Ester , Merino Fernando 
Published: January 2023

AbstractIt is well established in the literature that a firm’s innovation can promote its exports through demand-pull and/or regulatory channels. However, there is a lack of knowledge on whether green innovation strategies affect a firm’s international expansion. In this study, we depart from the existing literature by considering the introduction of environmental innovation as an antecedent factor that creates incentives for a firm to enter new markets and to be persistent in export activities. In particular, we investigate whether strategies aimed at reducing energy or water consumption and taking care of the environment have an impact on the probability of being an exporter and on the probability of continuing to export. The results, for a panel data of Spanish firms, confirm the existence of a premium on the probability of entering and remaining in international markets. Commitment to green innovation strategies, such as reductions in energy and water consumption or the minimization of environmental impact, generates a premium for companies that internalize their activities.
Upregulation ofPTPRCand Interferon Response Pathways in HIV-1 Seroconverters Prior to Infection
Authors: Li Yunqi , Lefebvre Francois , Nakku-Joloba Edith , Ronald Allan , Gray Glenda , de Bruyn Guy , Kiarie James , Celum Connie , Cameron Mark J , Lingappa Jairam R , Mackelprang Romel D 
Published: January 2023

AbstractHuman immunodeficiency virus 1 (HIV-1) exposed seronegative (HESN) individuals may have unique characteristics that alter susceptibility to HIV-1 infection. However, identifying truly exposed HESN is challenging. We utilized stored data and biospecimens from HIV-1 serodifferent couple cohorts, in which couples’ HIV-1 exposures were quantified based on unprotected sex frequency and viral load of the partner with HIV-1. We compared peripheral blood gene expression between 15 HESN and 18 seroconverters prior to infection. We found PTPRC (encoding CD45 antigen) and interferon-response pathways had significantly higher expression among individuals who went on to become seropositive and thus may be a signature for increased acquisition risk.
Plasma concentration of orally administered amoxicillin and clindamycin in patients receiving haemodialysis
Authors: Solli Camilla N , Bock Magnus , Kaur Kamal P , Kristensen Jonas H , Greibe Eva , Hansen Henrik P , Boesby Lene , Borg Rikke , Chaudry Mavish , Hoffmann-Lücke Elke , Moser Claus , Falkentoft Alexander C , Fosbøl Emil , Østergaard Lauge , Torp-Pedersen Christian , Bundgaard Henning , Iversen Kasper , Bruun Niels E 
Published: January 2023

AbstractObjectivesIn the randomized controlled trial PANTHEM, the prophylactic effect of oral amoxicillin or clindamycin is investigated in patients receiving chronic haemodialysis (HD). However, data on plasma concentrations of these antibiotics during HD are sparse. This study aims to determine if the plasma concentration of amoxicillin and clindamycin is sufficient during HD after oral administration of amoxicillin and clindamycin at three different time intervals prior to the HD procedure.MethodsAdult patients receiving chronic HD were investigated twice with an interval of at least 7 days starting with either a tablet of 500/125 mg amoxicillin/clavulanic acid or a tablet of 600 mg clindamycin. Patients were randomized to take the antibiotics either 30, 60 or 120 min prior to the HD procedure. Plasma antibiotic concentrations were measured at start, midway and at the end of HD. A lower threshold was set at 2.0 mg/L for amoxicillin and at 1.0 mg/L for clindamycin. In addition, a population pharmacokinetic (PK) analysis was performed, assessing PTA.ResultsIn the amoxicillin cohort (n = 37), 84% of patients and 95% of all plasma amoxicillin concentrations were above or at the threshold throughout the dialysis procedure. In the clindamycin cohort (n = 33), all concentrations were above the threshold throughout the dialysis procedure. Further, in all patients, the mean plasma concentration of both amoxicillin and clindamycin across the HD period was well above the threshold. Finally, the PK model predicted a high PTA in the majority of patients.DiscussionIn patients on chronic HD, oral administration of amoxicillin/clavulanic acid (500/125 mg) or clindamycin (600 mg) within 30–120 min prior to HD leads to a sufficient prophylactic plasma concentration across the HD period.