bluuebunny/crossref_metadata_2025_split huggingface dataset:
The hyperexcitability of laterodorsal tegmentum cholinergic neurons accompanies adverse behavioral and cognitive outcomes of prenatal stress
Authors: Shabani Mohammad , Ilaghi Mehran , Naderi Reyhaneh , Razavinasab Moazamehosadat 
Published: April 2023

AbstractExposure to prenatal stress (PS) leads to the offspring's vulnerability towards the development of cognitive and behavioral disorders. Laterodorsal tegmentum (LDT) is a part of the brainstem cholinergic system that is believed to play a pivotal role in the stress-associated progression of anxiety, memory impairment, and addictive behaviors. In this study, we aimed to investigate the electrophysiological alterations of LDT cholinergic neurons and its accompanied behavioral and cognitive outcomes in the offspring of mice exposed to physical or psychological PS. Swiss Webster mice were exposed to physical or psychological stress on the tenth day of gestation. Ex vivo investigations in LDT brain slices of adolescent male offspring were performed to evaluate the effects of two stressor types on the activity of cholinergic neurons. Open field test, elevated plus maze, passive avoidance test, and conditioned place preference were conducted to assess behavioral and cognitive alterations in the offspring. The offspring of both physical and psychological PS-exposed mice exhibited increased locomotor activity, anxiety-like behavior, memory impairment, and preference to morphine. In both early- and late-firing cholinergic neurons of the LDT, stressed groups demonstrated higher firing frequency, lower adaptation ratio, decreased action potential threshold, and therefore increased excitability compared to the control group. The findings of the present study suggest that the hyperexcitability of the cholinergic neurons of LDT might be involved in the development of PS-associated anxiety-like behaviors, drug seeking, and memory impairment.
Antimicrobial activity of cell free supernatants from probiotics inhibits against pathogenic bacteria isolated from fresh boar semen
Authors: Keeratikunakorn Krittika , Kaewchomphunuch Thotsapol , Kaeoket Kampon , Ngamwongsatit Natharin 
Published: April 2023

AbstractThe use of antibiotics with semen extender appears to be a practical solution to minimise bacterial growth in fresh boar semen preservation. Unfortunately, the excessive use of antibiotics promotes antimicrobial resistance (AMR). This becomes a worldwide concern due to the antimicrobial resistance genes transmitted to animals, environment, and humans. Probiotics are one of the alternative methods to reduce antibiotic use. They could inhibit pathogenic bacteria by producing antimicrobial substances in cell free supernatants (CFS). Nevertheless, there is no comprehensive study undertaken on inhibitory activity against pathogenic bacteria isolated from boar semen origin. Our study investigated the efficacy of CFS produced from selected probiotics: Bacillus spp., Enterococcus spp., Weissella spp., Lactobacillus spp., and Pediococcus spp. inhibiting pathogenic bacteria isolated from fresh boar semen. Besides, the semen-origin pathogenic bacteria are subjected to identification, antimicrobial resistance genes detection, and antibiotic susceptibility test (AST). Pseudomonas aeruginosa, Escherichia coli, and Proteus mirabilis are the most common pathogens identified in boar semen with resistance to numerous antibiotics used in pig industry. The CFS with its antimicrobial peptides and/or bacteriocin constituent derived from selected probiotics could inhibit the growth of pathogenic bacteria carrying antimicrobial resistance genes (mcr-3 and int1 genes). The inhibition zones for Pseudomonas aeruginosa, Escherichia coli, and Proteus mirabilis provided more efficient results in the CFS derived from Lactobacillus spp. and Pediococcus spp. than those of the CFS produced from Enterococcus spp., Weissella spp. and Bacillus spp., respectively. It is worth noted that as the incubation time increased, the antibacterial activity decreased conversely. Our results on CFS with its antimicrobial peptides and/or bacteriocin constituent inhibits semen-origin pathogenic bacteria guide the direction as a promising alternative method used in the semen extender preservation of the pig industry.
An intriguing approach toward antibacterial activity of green synthesized Rutin-templated mesoporous silica nanoparticles decorated with nanosilver
Authors: Abbasi Milad , Gholizadeh Razieh , Kasaee Seyed Reza , Vaez Ahmad , Chelliapan Shreeshivadasan , Fadhil Al-Qaim Fouad , Deyab Issa Farhan , Shafiee Mostafa , Zareshahrabadi Zahra , Amani Ali Mohammad , Mosleh-Shirazi Sareh , Kamyab Hesam 
Published: April 2023

AbstractIn recent years, mesoporous silica nanoparticles (MSNs) have been applied in various biomedicine fields like bioimaging, drug delivery, and antibacterial alternatives. MSNs could be manufactured through green synthetic methods as environmentally friendly and sustainable synthesis approaches, to improve physiochemical characteristics for biomedical applications. In the present research, we used Rutin (Ru) extract, a biocompatible flavonoid, as the reducing agent and nonsurfactant template for the green synthesis of Ag-decorated MSNs. Transmission electron microscopy (TEM), zeta-potential, x-ray powder diffraction (XRD), fourier transform infrared (FTIR) spectroscopy analysis, scanning electron microscopy (SEM), brunauer–emmett–teller (BET) analysis, and energy-dispersive system (EDS) spectroscopy were used to evaluate the Ag-decorated MSNs physical characteristics. The antimicrobial properties were evaluated against Staphylococcus aureus (S. aureus), Escherichia coli (E. coli), and also different types of candida. The cytotoxicity test was performed by using the MTT assay. Based on the findings, the significant antimicrobial efficacy of Ru-Ag-decorated MSNs against both gram positive and gram negative bacteria and different types of fungi was detected as well as acceptable safety and low cytotoxicity even at lower concentrations. Our results have given a straightforward and cost-effective method for fabricating biodegradable Ag-decorated MSNs. The applications of these MSNs in the domains of biomedicine appear to be promising.
Safety of oral propranolol for neonates with problematic infantile hemangioma: a retrospective study in an Asian population
Authors: Fu Ronghua , Zou Yun , Wu Zhiping , Jin Pingliang , Cheng Jun , Bai Hanxiang , Huang Mengyu , Huan Xiangquan , Yuan Hua 
Published: April 2023

AbstractAlthough the efficacy of propranolol in the treatment of infantile hemangioma (IH) has been well established, clinical data on the safety and tolerability of propranolol in neonates are still lacking. In this work, clinical data of 112 neonates with IH were analyzed retrospectively. All of the patients were evaluated in the hospital at the beginning of the treatment and later in outpatient settings during the treatment. Each time, the following monitoring methods were applied: physical examination, ultrasound echocardiography (UCG), electrocardiography (ECG), blood pressure (BP), heart rate (HR), and basic laboratory tests including blood glucose (BG), liver function, blood potassium, thyroid function. There was a significant reduction in BP and HR at the initiation of treatment. The incidences of bradycardia and hypoglycemia were observed to be increased with the prolong duration of treatment, but not prolonged PR interval. During the course of the therapy, the risk of hyperkalemia and hypothyroidism was reached maximum at the 2 months and 3 months, respectively. Physical growth index including average height, weight and head circumference was not influenced by the treatment. The observed adverse effects were majority mild and only 3 patients needed to rest for 7 days due to severe diarrhea before restarting treatment. This study demonstrated that propranolol is safe and well-tolerated by properly selected young infants with IH. No serious adverse events were observed.
Coxsackievirus A11 is an immunostimulatory oncolytic virus that induces complete tumor regression in a human non-small cell lung cancer
Authors: Sakamoto Akira , Inoue Hiroyuki , Miyamoto Shohei , Ito Shun , Soda Yasushi , Tani Kenzaburo 
Published: April 2023

AbstractNon-small cell lung cancer (NSCLC) is the leading cause of cancer-related mortality worldwide. Innovative treatment is required to improve overall survival rates for advanced NSCLC. Oncolytic virotherapy using enteroviruses has emerged as a promising anticancer strategy. To identify a novel, potent virotherapy with an improved safety profile, we assessed the oncolytic activity of 28 enteroviral strains and focused on coxsackievirus A11 (CVA11). CVA11 infection caused extensive oncolytic activity in all three of the examined human NSCLC cell lines, with high intercellular adhesion molecule-1 (ICAM-1) expression associated with greater CVA11-induced cytotoxicity. In vitro inhibition analysis using a pan-caspase inhibitor and western blot detection of cleaved poly (ADP-ribose) polymerase (PARP) indicated that apoptosis partly contributed to CVA11-driven cytotoxicity. CVA11 infection-induced immunogenic cell death in vitro was strongly suggested by substantial calreticulin expression and release of high mobility group box-1 protein (HMGB1). Moreover, in vivo treatment of human NSCLC xenografts with intratumoral CVA11 injection caused complete tumor regression in all treated mice, without significant weight loss. Our findings indicate that novel oncolytic virotherapy utilizing CVA11 may be less toxic and more effective than current treatments for human NSCLC, thus warranting further investigation in clinical trial settings, especially in combination with immunotherapy.
Direct evidence of terahertz emission arising from anomalous Hall effect
Authors: Mottamchetty Venkatesh , Rani Parul , Brucas Rimantas , Rydberg Anders , Svedlindh Peter , Gupta Rahul 
Published: April 2023

AbstractA detailed understanding of the different mechanisms being responsible for terahertz (THz) emission in ferromagnetic (FM) materials will aid in designing efficient THz emitters. In this report, we present direct evidence of THz emission from single layer Co$$_{0.4}$$

0.4

Fe$$_{0.4}$$

0.4

B$$_{0.2}$$

0.2

(CoFeB) FM thin films. The dominant mechanism being responsible for the THz emission is the anomalous Hall effect (AHE), which is an effect of a net backflow current in the FM layer created by the spin polarized current reflected at the interfaces of the FM layer. The THz emission from the AHE-based CoFeB emitter is optimized by varying its thickness, orientation, and pump fluence of the laser beam. Results from electrical transport measurements show that skew scattering of charge carriers is responsible for the THz emission in the CoFeB AHE-based THz emitter.
CFD model to study PM$$_{\textrm{10}}$$ dispersion in large-scale open spaces
Authors: Fernández-Pacheco V. M. , Álvarez-Álvarez E. , Blanco-Marigorta E. , Ackermann T. 
Published: April 2023

AbstractAir pollution has become a major concern in industrial or highly populated areas. Although legislation has been enacted to limit pollution levels, air quality monitoring still needs to be carried out by stations which are located at fixed points unable to provide the spatial evolution of pollutants. This research, focused on the city of Gijón (Asturias), includes a Computational Fluid Dynamics model capable of simulating the dispersion of pollutants in a large urban environment (12x18 km$$^{\textrm{2}}$$

2

). Different wind conditions were simulated with two sources of emission. The results show the influence of the terrain on the dispersion of pollutants in open spaces whilst simultaneously scrutinizing the origin of diffuse industrial pollution circulating over the city of Gijon. The simulation allows us to set limits in the areas with higher levels of contamination or to analyse the variations of particle concentration in height. Therefore, this research defines and validates a methodology to generate numerical models which grant us the opportunity to observe the spatial evolution of pollutants in large areas. This result endorses further use in other lines of research, such as the evaluation of corrective measures to improve air quality in highly polluted environments.
Prediction prolonged mechanical ventilation in trauma patients of the intensive care unit according to initial medical factors: a machine learning approach
Authors: Vali Mohebat , Paydar Shahram , Seif Mozhgan , Sabetian Golnar , Abujaber Ahmad , Ghaem Haleh 
Published: April 2023

AbstractThe goal of this study was to develop a predictive machine learning model to predict the risk of prolonged mechanical ventilation (PMV) in patients admitted to the intensive care unit (ICU), with a focus on laboratory and Arterial Blood Gas (ABG) data. This retrospective cohort study included ICU patients admitted to Rajaei Hospital in Shiraz between 2016 and March 20, 2022. All adult patients requiring mechanical ventilation and seeking ICU admission had their data analyzed. Six models were created in this study using five machine learning models (PMV more than 3, 5, 7, 10, 14, and 23 days). Patients’ demographic characteristics, Apache II, laboratory information, ABG, and comorbidity were predictors. This study used Logistic regression (LR), artificial neural networks (ANN), support vector machines (SVM), random forest (RF), and C.5 decision tree (C.5 DT) to predict PMV. The study enrolled 1138 eligible patients, excluding brain-dead patients and those without mechanical ventilation or a tracheostomy. The model PMV > 14 days showed the best performance (Accuracy: 83.63–98.54). The essential ABG variables in our two optimal models (artificial neural network and decision tree) in the PMV > 14 models include FiO2, paCO2, and paO2. This study provides evidence that machine learning methods outperform traditional methods and offer a perspective for achieving a consensus definition of PMV. It also introduces ABG and laboratory information as the two most important variables for predicting PMV. Therefore, there is significant value in deploying such models in clinical practice and making them accessible to clinicians to support their decision-making.
ALS-linked TDP-43 mutations interfere with the recruitment of RNA recognition motifs to G-quadruplex RNA
Authors: Ishiguro Akira , Ishihama Akira 
Published: April 2023

AbstractTDP-43 is a major pathological protein in sporadic and familial amyotrophic lateral sclerosis (ALS) and mediates mRNA fate. TDP-43 dysfunction leads to causes progressive degeneration of motor neurons, the details of which remain elusive. Elucidation of the molecular mechanisms of RNA binding could enhance our understanding of this devastating disease. We observed the involvement of the glycine-rich (GR) region of TDP-43 in the initial recognition and binding of G-quadruplex (G4)-RNA in conjunction with its RNA recognition motifs (RRM). We performed a molecular dissection of these intramolecular RNA-binding modules in this study. We confirmed that the ALS-linked mutations in the GR region lead to alteration in the G4 structure. In contrast, amino acid substitutions in the GR region alter the protein structure but do not void the interaction with G4-RNA. Based on these observations, we concluded that the structural distortion of G4 caused by these mutations interferes with RRM recruitment and leads to TDP-43 dysfunction. This intramolecular organization between RRM and GR regions modulates the overall G4-binding properties.
Stabilization of a chaotic oscillator via a class of integral controllers under input saturation
Authors: Aguilar-López Ricardo , Mata-Machuca Juan L. 
Published: April 2023

AbstractThis work presents the straightforward design of an integral controller with an anti-windup structure to prevent undesirable behavior when actuator saturation is considered, and the proposed controller improves the performance of the closed-loop dynamics of a class of nonlinear oscillators. The proposed integral controller has an adaptive control gain, which includes the absolute value of the named control error to turn off the integral action when it is saturated. Closed-loop stability analysis is performed under the Lyapunov theory framework, where it can be concluded that the system behaves in an asymptotically stable way. The proposed methodology is successfully applied to a Rikitake-type oscillator, considering a single input-single output (SISO) structure for regulation and tracking trajectory purposes. For comparison, an equivalent fixed gain integral controller is also implemented to analyze the corresponding anti-windup properties of the proposed control structure. Numerical experiments are conducted, showing the superior performance of the proposed controller.
Bypassing misinformation without confrontation improves policy support as much as correcting it
Authors: Calabrese Christopher , Albarracín Dolores 
Published: April 2023

AbstractCurbing the negative impact of misinformation is typically assumed to require correcting misconceptions. Conceivably, however, bypassing the misinformation through alternate beliefs of opposite implications may reduce the attitudinal impact of the misinformation. Three experiments, one preregistered with a sample representative of the United States population, examined the impact of (a) directly correcting prior misinformation offered in support of restricting Genetically Modified (GM) foods (i.e., the correction strategy) and (b) discussing information in support of GM foods (i.e., the bypassing strategy), compared to a misinformation-only control condition. Findings consistently revealed that bolstering beliefs with opposite implications is just as effective at reducing opposition to GM foods as is correcting misinformation about GM foods. Thus, bypassing should be added to our arsenal of methods to curb the impact of misinformation.
The uneven impact of climate change on drought with elevation in the Canary Islands
Authors: Carrillo Judit , Hernández-Barrera Sara , Expósito Francisco J. , Díaz Juan P. , González Albano , Pérez Juan C. 
Published: April 2023

AbstractClimate models project an increase in drought and aridity in many regions in response to greenhouse gas concentrations in the atmosphere. In areas with complex topography, such as the Canary Islands, elevation gradients may play an important role in future changes. Convection-permitting climate simulations driven by data from three global climate models included in the Coupled Model Intercomparison Project (CMIP5) have been performed for the Canary Islands. A significant increase in the duration and severity of drought is projected by the end of the twenty-first century (2070–2099), relative to the recent past (1980–2009), under intermediate and high emissions scenarios. In addition, the percentage of land affected by droughts, on average, would increase considerably, covering up to 96% in the higher elevations, in the business-as-usual scenario. These changes and the increase in aridity are more pronounced at higher altitudes due to a clear dependence of temperature rise as a function of elevation and a substantial decrease in precipitation.
Atlantic cod (Gadus morhua) larvae are attracted by low-frequency noise simulating that of operating offshore wind farms
Authors: Cresci Alessandro , Zhang Guosong , Durif Caroline M. F. , Larsen Torkel , Shema Steven , Skiftesvik Anne Berit , Browman Howard I. 
Published: April 2023

AbstractThe number and size of offshore wind (OW) turbines is increasing rapidly. OW turbines produce continuous, low-frequency noise that could impact marine fish dispersing/migrating through the facilities. Any such impact would be relevant for larval stages, which have limited possibility to swim away from OW facilities. If directional movement of fish larvae at sea is impacted by low-frequency continuous sound is unknown. We observe the behavior of Atlantic cod larvae (N = 89) in response to low-frequency sound while they are drifting in a Norwegian fjord inside transparent drifting chambers. We transmit 100 Hz continuous sound in the fjord, in the intensity range of OW turbines’ operational noise, and measure the sound pressure and 3-D particle motion. Half of the larvae (N = 45) are exposed to low-frequency (100 Hz) continuous sound, while the other half (N = 44) are observed under the same conditions but without the sound. Exposure does not affect the routine and maximum swimming speeds or the turning behavior of the larvae. Control larvae orient to the northwest. In contrast, exposed larvae orient towards the source of low-frequency sound and particle motion. This provides a basis to assess how OW might impact dispersal in this species.
Tonian carbonaceous compressions indicate that Horodyskia is one of the oldest multicellular and coenocytic macro-organisms
Authors: Li Guangjin , Chen Lei , Pang Ke , Tang Qing , Wu Chengxi , Yuan Xunlai , Zhou Chuanming , Xiao Shuhai 
Published: April 2023

AbstractMacrofossils with unambiguous biogenic origin and predating the one-billion-year-old multicellular fossils Bangiomorpha and Proterocladus interpreted as crown-group eukaryotes are quite rare. Horodyskia is one of these few macrofossils, and it extends from the early Mesoproterozoic Era to the terminal Ediacaran Period. The biological interpretation of this enigmatic fossil, however, has been a matter of controversy since its discovery in 1982, largely because there was no evidence for the preservation of organic walls. Here we report new carbonaceous compressions of Horodyskia from the Tonian successions (~950–720 Ma) in North China. The macrofossils herein with bona fide organic walls reinforce the biogenicity of Horodyskia. Aided by the new material, we reconstruct Horodyskia as a colonial organism composed of a chain of organic-walled vesicles that likely represent multinucleated (coenocytic) cells of early eukaryotes. Two species of Horodyskia are differentiated on the basis of vesicle sizes, and their co-existence in the Tonian assemblage provides a link between the Mesoproterozoic (H. moniliformis) and the Ediacaran (H. minor) species. Our study thus provides evidence that eukaryotes have acquired macroscopic size through the combination of coenocytism and colonial multicellularity at least ~1.48 Ga, and highlights an exceptionally long range and morphological stasis of this Proterozoic macrofossils.
Role of urban vegetation in air phytoremediation: differences between scientific research and environmental management perspectives
Authors: Gong Cheng , Xian Chaofan , Wu Tong , Liu Jingru , Ouyang Zhiyun 
Published: April 2023

AbstractAir pollutant removal by urban vegetation is perceived to be a key ecosystem service for mitigating air pollution. However, the effectiveness of air phytoremediation in cities requires more synthesis to inform environmental management. A Bayesian meta-analysis approach was used to quantify the effectiveness of the removal of typical air pollutants—particle matter (PM), nitrogen oxide (NOx), sulfur dioxide (SO2) and ozone (O3)—by synthesizing global field measurement studies. The results revealed that urban vegetation can mitigate the growth of air pollutant concentrations, with reduction rates of 16.5~26.7% for PM, 13.9~36.2% for NOx, and 20.5~47.8% for SO2. However, they failed to significantly mitigate ground-level O3, corresponding to an increase of 5.1~25.9%. The variability in effect sizes was mainly influenced by the distance to nearest highway, ambient concentration, relative humidity, and green coverage. A questionnaire survey conducted in Shenzhen city (China) showed that most environmental managers supposed positive impacts of urban vegetation on all four air pollutants, which was at odds with our findings with respect to O3. This study can inform the lessening of discrepancies between scientific research and environmental managers’ perceptions on how to improve air phytoremediation for urban air pollution mitigation in China and elsewhere.