bluuebunny/crossref_metadata_2025_split huggingface dataset:
Resistance training rejuvenates aging skin by reducing circulating inflammatory factors and enhancing dermal extracellular matrices
Authors: Nishikori Shu , Yasuda Jun , Murata Kao , Takegaki Junya , Harada Yasuko , Shirai Yuki , Fujita Satoshi 
Published: May 2023

Abstract
Aerobic training (AT) is suggested to be an effective anti-aging strategy for skin aging. However, the respective effects of resistance training (RT) have not been studied. Therefore, we compared the effects of AT and RT on skin aging in a 16-week intervention in 61 healthy sedentary middle-aged Japanese women. Data from 56 women were available for analysis. Both interventions significantly improved skin elasticity and upper dermal structure, and RT also improved dermal thickness. After the training intervention, expression of dermal extracellular matrix-related genes was increased in normal human primary dermal fibroblasts. AT and RT had different effects on circulating levels of factors, such as cytokines, hormones in serum, and metabolites, and RT increased dermal biglycan (BGN). To our knowledge, this is the first report to show different effects of AT and RT on skin aging and identify the key factors involved in RT-induced skin rejuvenation.
Nasogastric tube after laparoscopic Heller-Dor myotomy: do you really need it?
Authors: Palomba Giuseppe , Basile Raffaele , Capuano Marianna , Pesce Marcella , Rurgo Sara , Sarnelli Giovanni , De Palma Giovanni Domenico , Aprea Giovanni 
Published: May 2023

Abstract

Background
Achalasia is a rare esophageal motor disorder. Laparoscopic Heller-Dor myotomy (LHD) is among the most effective treatments. The use of nasogastric tube (NGT) following LHD is still controversial. The aim of our study was to evaluate the role of NGT in achalasia patients undergoing LHD.
Methods
We prospectively enrolled achalasia patients treated with LHD from January 2019 until June 2022 at our institution and randomly assigned them to two groups: one group with NGT and group without (noNGT). Perioperative outcomes were compared between the two groups. ClinicalTrials.gov Identifier: NCT05729971.
Results
Seventy-nine patients who underwent LHD myotomy were prospectively enrolled. Patients were divided in two groups: 38 with NGT and 41 noNGT. There were no significant differences between two groups in terms of demographic data, preoperative characteristics, length of stay, start of liquid and solid diet, intraoperative and postoperative complications. First flatus was shorter in the noNGT group (p: 0.03).
Conclusion
Nasogastric tube placement following LHD myotomy does not reduce nausea, vomiting and chest pain, while it increased patients discomfort and time of first flatus.
Novel XIAP mutation in early-onset Crohn’s disease complicated with Acute Heart Failure: a case report
Authors: Peng Chendong , Jiang Yuang , Ou Xianhong , Liao Lei , Yang Chengying , Zhou Qiao , Wei Yan , Chang Lijia , Fan Xinrong 
Published: May 2023

Abstract
Background
The X-linked inhibitor of apoptosis (XIAP) protein is encoded by the XIAP gene and is critical for multiple cell responses. XIAP plays a role in preventing cell death.We report the clinical features and results of hemizygous mutation of the XIAP gene in a young male died due to Crohn’s disease complicated with Acute Heart Failure.A young male of 16 years of age died due to no available therapy.
Case presentation
A young male of 16 years of age was initially diagnosed with Crohn's disease based on evidence from endoscopic and histological findings. Although supporting care, anti-infective drugs and biologics were applied consecutively for 11 months, his clinical manifestations and laboratory indices (patient’s condition) were not improved. This was followed by poor a nutritional status and sustained weight loss. Subsequently, acute heart failure ledto exacerbation of the patient’s condition. Therefore, he was diagnosed with wet beriberi according to thiamine deficiency, but standard medical therapy for heart failure and thiamine supplementation did notreverse the adverse outcomes. Finally, comprehensive genetic analysis of peripheral blood-derived DNA revealed a novel hemizygous mutation of the XIAP gene (c.1259_1262 delACAG), which was inherited from his mother.
Conclusion
The novel XIAP mutation (c.1259_1262 delACAG) was identified in this study. It may be an important cause of Crohn's disease and plays an important role in the progression of heart failure. Additionally,thiamine deficiency triggers avicious cycle.
WITHDRAWN: Paired stress granule gene signatures predict survival and inform immune status in hepatocellular carcinoma
Authors: Li Zhaosheng , Pei Lei , Luo Jia , Zhang Sai , Tian Xuefei , Tao Yiming 
Published: May 2023

Abstract
Background
Stress granules formation is closely associated with the progression of hepatocellular carcinoma (HCC). Factors determination this process remain to be elucidated. In this study, stress granule-related genes were validated as a predictor of HCC.
Methods
The stress granules-related associated genes were collected from the MSGP database and the MsigDB database. A novel prognostic risk scoring model were constructed by paired gene signature method. We identified eukaryotic translation initiation factor 4A3 (EIF4A3) and karyopherin subunit alpha 2 (KPNA2) as candidate prognostic biomarkers, and their correlation with both prognosis and immune infiltration in HCC were evaluated. The expression of EIF4A3 and KPNA2 in HCC tissues was detected through immunohistochemistry (IHC).
Results
Through pairing of all DESG gene, we obtained a total of 16251 significance pairs. Subsequently, 93 pairs of all pairs containing EIF4A3 were extracted in this research. As a stress granule formation regulator, KPNA2 displayed the greatest correlations with EIF4A3 in HCC. Hyperactivated EIF4A3 and KPNA2 is associated with the poor clinical outcome of HCCs after hepatic resection. Involvement of EIF4A3 and KPNA2 in immune infiltration have been showed.
Conclusion
Our study identified coexistence of EIF4A3 and KPNA2 dysregulation inform poor clinical outcomes in HCC.
Chemotaxonomy for female feeding stages of the diseases vector tick Rhipicephalus annulatus (Acari: Ixodidae) based on cuticle hydrocarbons
Authors: Amer Asmaa , Okely Mohammed , Gadallah Sohair , Ali Asmaa Ali Baioumy 
Published: May 2023

Abstract

Rhipicephalus annulatus is a vector of bovine babesiosis (cattle fever) and bovine anaplasmosis that infect approximately 80% of cattle production, leading to loss in the livestock and dairy industry. They are vectors of disease agents to humans. Cuticular hydrocarbons (CHCs) have been used as a chemotaxonomic tool to facilitate the identification of various arthropod species since the 1970s. The present study used GC-MS to differentiate the CH components of feeding stages of female R. annulatus. Hydrocarbons identified from the three stages contain 7 to 44 carbons and include linear strait-chained alkanes, and a few branched alkanes and alkenes. The chemical diversity was higher in an unfed stage and lower in an engorged one. The most abundant compounds across all stages were eicosane and tetracosane. Principal component and cluster analyses revealed that engorged stages were the most dissimilar stage in terms of their CHC profile, while the unfed and semi-fed stages showed relatively similar CHC profiles. The results obtained in this study demonstrated the potential use of CHCs to identify species, and different stages for female R. annulatus, determination of the most affected stage to be easily controlled, and establishment of a database to be used in chemotaxonomy of tick species.
Knee osteonecrosis after SARS-CoV-2 infection: a systematic case-based review
Authors: Za Pierangelo , Papalia Giuseppe Francesco , Russo Fabrizio , Vasta Sebastiano , Vadalà Gianluca , Papalia Rocco 
Published: May 2023

Abstract

Background: SARS-CoV-2 is the virus responsible for COVID-19. Patients with Covid-19 manifested symptoms mainly related to the respiratory system, but also the musculoskeletal system can be involved. Covid-19 has been described as a possible cause of knee osteonecrosis (ON). We performed a systematic review to investigate the hypothetical correlation between Covid-19 and knee ON.
Methods: Inclusion criteria were all articles reporting cases of knee ON after a diagnosis of SARS-CoV-2 infection. Considering that Covid-19 is an emerging disease, we included all levels of evidence studies.
Results: We finally included 2 case series and 2 case reports. We extracted data regarding demographic and clinical characteristics, details of magnetic resonance imaging (MRI), use of corticosteroids (CCS), temporal correlation between ON and Covid-19, treatment of the lesion and its outcomes. A total of 6 cases of post-Covid knee ON have been described. Knee pain arose on average 8 weeks after the diagnosis of Covid-19. All patients had knee MRI showing osteonecrosis. CCS were used to treat COVID-19-related symptoms in 3 cases. The medial femoral condyle was always involved by ON. Conservative treatment was successful in 4 patients.
Conclusions: It is unclear the correlation between COVID-19 and ON. Probably post Covid-19 osteonecrosis has a multifactorial origin in which factors related to the patient, consequences of Covid-19 and CCS therapy add up to cause a reduction of blood supply and bone vitality until ON is triggered. A greater number of patients is needed to clarify the role of Covid-19 in the etiopathogenesis of knee ON.
Non-stapled, Total Laparoscopic Roux-en-Y Anastomosis: A Safe and Effective Procedure for Radical Pediatric Choledochal Cyst Excision
Authors: Cheng Jiwen , Yang Weili , Yu Qiang , Fu JiaLu , Li Peng 
Published: May 2023

Abstract
Background
Laparoscopic or robot-assisted surgery is the main minimally invasive surgery for pediatric choledochal cyst (CDC). Most surgeries have involved extracorporeal creation of the Roux-en-Y jejunal limb; a few surgeries have been performed by intracorporeal creation of the jejunal limb with an endoscopic stapler. We aimed to assess the safety and effectiveness of laparoscope-assisted Roux-en-Y jejunal limb created intracorporeally by hand-sewing in pediatric minimally invasive surgery for a choledochal cyst (CDC).
Methods
Patients diagnosed with CDC between January 2020 and April 2022 were included in this retrospective analysis. We collected data on clinical characteristics, operative details, time for performing laparoscopic Roux-en-Y anastomosis without stapling, and postoperative outcomes.
Results
Hand-sewn laparoscope-assisted Roux-en-Y jejunal limb was performed successfully for 21 patients. The median patient age was 5.8 years. The median age at surgery was 3.3 ± 3.1 years (range 26 days-13.6 years). The types of cysts included 12 Ia, 7 Ic, and 2 IVa. The mean operative duration was 216.5 ± 25.2 min, intraoperative bleeding volume was 7 ± 2.3 ml, average postoperative time to start oral intake was 2.1 ± 0.4 days, average postoperative indwelling drainage tube time was 2.6 ± 1.1 days, and postoperative hospital stay was 5.3 ± 1.1 days. The follow-up period was 6 to 25 months. No patient incurred complications, e.g., anastomotic stenosis or fistula, pancreatitis, cholangitis, or death.
Conclusions
Total laparoscope-assisted construction of Roux-en-Y limb without stapling, excision of the cyst, and hepaticojejunostomy are feasible and safe in the treatment for pediatric CDC. This procedure may also have the following advantages: rapid recovery of postoperative gastrointestinal function, short hospitalization, no patient age limit, and no additional hospitalization costs.
Black phosphorus-incorporated novel Ti-12Mo-10Zr implant for multimodal treatment of osteosarcoma
Authors: Cai Bianyun , Huang Leizhen , Zhou Xueke , Zhou Xuan , Lei Kun , Han Meng , Zhang Zilin , Li Xiaofang , Li Guangda 
Published: May 2023

Abstract
The repair and reconstruction of large bone defects after bone tumor resection is still a great clinical challenge. At present, orthopedic implant reconstruction is the mainstream treatment for repairing bone defects. However, according to clinical feedback, local tumor recurrence and nonunion of bone graft are common reasons leading to the failure of bone defect repair and reconstruction after bone tumor resection, which seriously threaten the physical and mental health of patients. On this basis, here the self-developed low modulus Ti-12Mo-10Zr alloy (TMZ) was chosen as substrate material. To improve its biological activity and osteointegration, calcium, oxygen, and phosphorus co-doped microporous coating was prepared on TMZ alloy by microarc oxidation (MAO). Then, black phosphorus (BP) nanosheets were incorporated onto MAO treated TMZ alloy to obtain multifunctional composites. The obtained BP-MAO-TMZ implant exhibited excellent photothermal effects and effective ablation of osteosarcoma cancer cells under the irradiation of 808 nm near infrared laser, while no photothermal or therapeutic effects were observed for TMZ alloy. Meanwhile, the structure/component bionic coating obtained after MAO treatment as well as the P-driven in situ biomineralization performance after incorporation of BP nanosheets endowed BP-MAO-TMZ implant with synergistic promoting effect on MC3T3-E1 osteoblasts’ activity, proliferation and differentiation ability. This study is expected to provide effective clinical solutions for problems of difficult bone regeneration and tumor recurrence after tumor resection in patients with bone tumors, and to solve a series of medical problems such as poor prognosis and poor postoperative quality of patients life with malignant bone tumors.
Defect-driven nanostructuring of low-nuclearity Pt-Mo ensembles for continuous gas-phase formic acid dehydrogenation
Authors: Guo Luyao , Zhuge Kaixuan , Yan Siyang , Wang Shiyi , Zhao Jia , Wang Saisai , Qiao Panzhe , Liu Jiaxu , Mou Xiaoling , Zhu Hejun , Zhao Ziang , Lin Ronghe , Ding Yun (J) 
Published: May 2023

Abstract
Supported metal clusters comprising of well-tailored low-nuclearity heteroatoms have great potentials in catalysis owing to the maximized exposure of active sites and metal synergy. However, atomically precise design of these architectures is still challenging for the lack of practical approaches. Herein, we report a defect-driven nanostructuring strategy through combining defect engineering of nitrogen-doped carbons and sequential metal depositions to prepare a series of Pt and Mo ensembles ranging from single atoms to sub-nanoclusters. When applied in continuous gas-phase decomposition of formic acid, the low-nuclearity ensembles with unique Pt3Mo1N3 configuration deliver CO-free hydrogen at full conversion with unexpected high activity of 0.62 molHCOOH molPt−1 s−1 and remarkable stability, significantly outperforming the previously reported catalysts. The remarkable performance is rationalized by a joint operando dual-beam Fourier transformed infrared spectroscopy and density functional theory modeling study, pointing to the Pt-Mo synergy in creating a new reaction path for consecutive HCOOH dissociations.
Core competencies for dermatology physician assistants: knowledge recommendations from a national survey of dermatologists and physician assistants
Authors: Young Peter A. , Bae Gordon H. , Pettey Adam A. , Nguyen Andrea , Young R. John , McCleskey Patrick E. 
Published: May 2023

Abstract
The specialty of dermatology has no formal competency guidelines for its physician assistant (PA) workforce, which doubled in the past decade and is the 5th-largest of any specialty. We set out to delineate potential core competencies for dermatology PAs by surveying dermatologists and PAs. A survey was sent to 97 PAs and 142 dermatologists, consisting of 149 dermatologic diagnoses and systemic medications. Participants were asked to rate items on a sliding scale of proficiency, according to the knowledge they expect of PAs after 2 years of dermatology employment. Participants were solicited via email, phone, and in-person at professional functions. Contact information was obtained from clinic websites, corresponding authors of relevant articles, and the American Medical Association’s directory of dermatology residencies. Clinicians without 2 years of relevant experience were excluded. Clinicians recorded their license type, years practicing dermatology, state of licensure, and optional comments. Data were collected between 01/10/2022-02/28/2023. Diagnoses and medications eliciting strong recommendations for comprehensive understanding from both dermatologists and PAs, discrepancies between each cohorts’ responses, and variability of individual answers. The response rate was 72%. At least 50% of the 79 dermatologists and 79 PAs who met inclusion criteria answered “start treatment” or “manage most” for 67 and 91 of 102 diagnoses, respectively. At least 50% of each expected proficiency for most common infections, eruptions, benign lesions, and non-melanoma skin cancers. At least 50% of dermatologists and PAs recommended strong knowledge for 32 and 39 of 47 medications, respectively. Free-typed comments were submitted by 22 dermatologists and 23 PAs; these mutually endorsed the dermatologist-led care team model and highlighted the heterogeneous nature of the current workforce’s aptitudes, citing variation of individuals and settings. This information could serve interprofessional efforts toward establishing nationally-recognized clinical competencies, thereby facilitating more uniformly excellent patient care from PAs on dermatologist-led teams. Educational needs for early-career PAs will vary by institution; thoughtfully-planned standards might formally enable competence demonstration in specific areas of dermatology.
Tunable narrowband and broadband coexisting absorber enabled by a simple all-metal grating for sensing applications
Authors: Wang Jianwei , Zhang Ze , Lin Zhiyan , Li Hanting , Fan ZhenJun , Gao Hua 
Published: May 2023

Abstract
We realize a tunable narrowband and broadband coexisting absorber based on a simple step-shaped all-metal grating structure. The absorber presents an ultra-narrow absorption band of 1.5 nm and a relatively broad absorption band of 29.8 nm, both with nearly 100% absorption in the infrared region. The mechanism underlying the dual-band perfect absorption is the interaction between two diffraction coupled surface plasmon polariton (SPP) modes with one of them modulated by a cavity resonance. Influences of structure parameters on the absorption performance are numerically investigated. It is found that the positions of the two perfect absorption peaks can be easily tuned both independently and together by changing the structural parameters. In addition, the designed grating structure presents excellent sensing performance with sensitivity and figure of merit as high as 2514 nm/RIU and 1600RIU-1, respectively. Such high sensing metrics arise from the poor field localization and the ultra-narrow absorption band enabled by the diffraction coupled SPP modes as well as the specially designed all-metal structure. The excellent sensing performance, flexible tenability and simple structure design endow this grating absorber with great potential in high-precision biochemical sensing applications.
Effect of intraoperative use of muscle relaxants on postoperative pharyngeal discomfort after intubation anesthesia: a systematic review and meta-analysis
Authors: Wang Yan , Xu Cong , Zhao He , Liang Tiantian , Liu Sha , Qiu Jingjing , Song Xicheng , Sun Yan , Liu Limei 
Published: May 2023

Abstract

Background
General anesthesia with intubation is widely used for surgery. The factors responsible for pharyngeal discomfort after intubation need to be identified. This meta-analysis aimed to clarify the relationship between intraoperative use of muscle relaxants and postoperative pharyngeal discomfort in patients, which is important for postoperative perioperative care.
Methods
Publications in English were manually searched. After evaluating the quality of selected studies, a meta-analysis was conducted using Review Manager 5.4 software and Endnote. Three reviewers independently selected the data from the selected studies.
Results
In the included literatures, muscle relaxants were used in the experimental group and normal saline was used in the control group. Ten randomized controlled trials (with a total of 1358 patients) were included in this meta-analysis. The risk of pharyngalgia and cough was lower in patients receiving muscle relaxants than in controls (RR = 0.67, 95% CI: 0.53 to 0.86, P = 0.002; RR = 0.053, 95% CI: 0.007 to 0.381, P = 0.004). Risk of sore throat, and hoarseness were not significantly different between those receiving muscle relaxants and controls (RR = 0.92, 95% CI: 0.68 to 1.23, P = 0.65; RR = 1.01, 95% CI: 0.81 to 1.27, P = 0.92). The preoperative first pass success was significantly higher in patients receiving muscle relaxants than in control patients (RR = 0.37, 95% CI: 0.14 to 0.98, P = 0.05).
Conclusion
The use of muscle relaxants during intubation anesthesia effectively reduced the development of upper respiratory complications, which also ruled out a negative effect of muscle relaxants in some patients with postoperative upper respiratory complications; however, the incidence of postoperative sore throat and hoarseness was quite high. However, the incidence of postoperative sore throat and hoarseness is quite high and requires continued exploration
StayGold variants for molecular fusion and membrane targeting applications
Authors: Ando Ryoko , Shimozono Satoshi , Ago Hideo , Takagi Masatoshi , Sugiyama Mayu , Kurokawa Hiroshi , Hirano Masahiko , Ueno Go , Ishidate Fumiyoshi , Fujiwara Takahiro , Okada Yasushi , Yamamoto Masaki , Miyawaki Atsushi 
Published: May 2023

Abstract

Although StayGold is a bright and highly photostable fluorescent protein (FP), its obligate dimer formation may prevent its application in molecular fusion and membrane targeting. With the objective of attaining monovalent as well as bright and photostable labeling, we engineered tandem dimers of StayGold to be dispersible. On the basis of the crystal structure of this FP, we disrupted the dimer interface to develop monomeric variants of StayGold. We applied the new StayGold tools to live cell imaging experiments using spinning-disk laser scanning confocal microscopy or structured illumination microscopy. We achieved cell-wide, high–spatiotemporal-resolution, and sustained imaging of subcellular dynamic events, including the targeting of endogenous condensin I to mitotic chromosomes at the onset of mitosis, the movement of the Golgi apparatus and its membranous derivatives along microtubule networks, the distribution of cortical filamentous actin near the plasma membrane, and the remolding of cristae membranes within mobile mitochondria.
Neurophysiology of pain for non-neurophysiologists: a systematic review
Authors: Ramne Malin , Elam Mikael , Löken Line S. , Ortiz-Catalan Max 
Published: May 2023

Abstract

This systematic literature review aims at providing a concise, yet comprehensive overview of the neurophysiology of pain for non-neurophysiologists. In particular, we focused on the neurophysiological processes involved in the transduction, transmission, modulation, and perception of acute pain. A systematic literature search was performed using the PubMed-database on October 30th, 2020. After screening for inclusion/exclusion, we selected 79 articles to be included in this review. The primary intention of this review is to serve as base upon which mathematical and computational models of the pain system can be built. For this purpose, we present the consensus and disagreements in the field in a comprehensive manner. We also hope that it can provide insight for other professionals interested on pain, who do not have a background in neurophysiology.