bluuebunny/crossref_metadata_2025_split huggingface dataset:
Urinary iodine concentration and its associations with thyroid function in pregnant women of Shanghai
Authors: Wu Yiming , Yang Jie , Su Qing , Gu Hongxia , Qin Li 
Published: July 2023

ObjectiveTo assess the iodine status and its associations with thyroid function in pregnant women of Shanghai.MethodsIn this cross-sectional study, a total of 562 pregnant women were enrolled from January to December 2021. Both serum thyroid-stimulating hormone (TSH), free triiodothyronine (FT3), free thyroxine (FT4), thyroid peroxidase antibody (TPOAB), thyroglobulin antibody (TGAB), and urinary iodine concentration (UIC) were detected. Participants were divided into four groups based on their UIC values. Correlation analysis was used to investigate the association between UIC and thyroid function-associated parameters.ResultsThe median UIC of the pregnant women studied was 158.25µg/L (interquartile range [IQR] 90.15, 245.65µg/L). Among all the subjects, 45.55% had iodine deficiency according to the World Health Organization (WHO) criteria, and 15.65% had thyroid autoimmunity. FT3, FT4, TSH, TPOAB and TGAB levels were not different among different UIC groups (P &gt; 0.05). UIC and TSH were negatively correlated (r=-0.127, p=0.043) in UIC&lt;150 µg/L group. In the group with UIC 250- 499 µg/L, UIC was positively correlated with total T4 (TT4), total T3 (TT3) and TPOAB (r= 0.228, p=0.022, r=0.208, p= 0.039, r=0.190, p=0.042, respectively). A negative correlation between UIC and TPOAB values was observed in TPOAB-positive (+) pregnant women (r=-0.384, p=0.012). The prevalence of isolated hypothyroxinemia in UIC&lt;150 µg/L group was significantly higher than that of other groups(p=0.033). The relationship between the prevalence of thyroid diseases and UIC embodied a U-shaped curve.ConclusionPregnant women on Chongming Island of Shanghai were iodine sufficient during the second trimester, but iodine deficiency was still prevalent. Both low and high gestational iodine status was related to thyroid function and autoimmunity. Optimal iodine nutrition status during gestation was important.
Becoming adults: exploring the late ontogeny of the human talus
Authors: Figus Carla , Sorrentino Rita , Carlson Kristian J. , Colombo Antony , Bortolini Eugenio , Bernardini Federico , Erjavec Igor , Hajdu Tamás , Szeniczey Tamás , Mateovics-László Orsolya , Pap Ildiko , Tuniz Claudio , Novak Mario , Belcastro Maria Giovanna , Ryan Timothy M. , Benazzi Stefano 
Published: July 2023

IntroductionThe talus plays an important role in receiving and dissipating the forces and linking the leg and the foot. As such, it is of paramount importance to analyze how its morphology, internal and external, changes during late ontogeny and through adolescence.MethodTo explore both the external shape and the internal architecture of the talus, Geometric Morphometrics and trabecular analysis have been applied to a sample of 35 tali from modern human juveniles aged between 5 and 15 years old (Middle Neolithic (4800–4500 BCE) to mid-20th century).ResultsResults show that, as the overall size of the talus increases, the shape and orientation of talar facets also change. The youngest individuals exhibit a functional talus that is still characterized by a relatively immature shape (e.g., subtly expressed margins of articular surfaces) with articular facets only minimally rotated towards an adult configuration. In adolescents, talar shape has achieved adult form after the age of 11, with all the articular facets and posterior processes well-developed. Considering internal morphology, trabecular bone varies between age classes. While Bone Volume Fraction shifts during the age 5–15 range, Degree of Anisotropy is relatively more stable over the developmental period examined in the study since it exhibits smaller variations between age classes.DiscussionThis study examined the late ontogeny of the human talus by considering both internal and external morphology. Results suggest that, although the locomotion has already assumed an adult-like pattern, the exploration of late talar growth may help understand how the talus adapts to changes in locomotor activity and how it responds to the increase in weight. Present results can be used to a better understanding of talar plasticity, improving interpretations of adult human talar form.
Major fine-scale spatial heterogeneity in accumulation of gelatinous carbon fluxes on the deep seabed
Authors: Hoving Henk-Jan , Boetius Antje , Dunlop Katherine , Greinert Jens , Haeckel Matthias , Jones Daniel O. B. , Simon-Lledó Erik , Marcon Yann , Stratmann Tanja , Suck Inken , Sweetman Andrew K. , Purser Autun 
Published: July 2023

Abyssal plain communities rely on the overlying water column for a settling flux of organic matter. The origin and rate of this flux as well as the controls on its fine-scale spatial distribution following seafloor settlement are largely unquantified. This is particularly true across regions where anthropogenically-induced seafloor disturbance has occurred. Here, we observed, quantified and mapped a mass deposition event of gelatinous zooplankton carcasses (pyrosomes) in July-September 2015 across one such physically disturbed region in the Peru Basin polymetallic nodule province (4150 m). Seafloor in this area was disturbed with a plough harrow in 1989 (as part of the DISCOL experiment) causing troughs in the sediment. Other parts were disturbed with an epibenthic sled (EBS) during a cruise in 2015 resulting in steep-walled, U-shaped troughs. We investigated two hypotheses: a) gelatinous food falls contribute significantly to the abyssal plain carbon pump and b) physical seafloor disturbance influences abyssal distribution of organic matter. We combined optical and bathymetric seafloor observations, to analyze pyrosome distribution on seabeds with different levels of disturbance. 2954 pyrosome colonies and associated taxa were detected in &gt; 14,000 seafloor images. The mean regional carbon (C) deposition associated with pyrosome carcasses was significant compared to the flux of particulate organic C (182 to 1543%), and the total respired benthic C flux in the DISCOL Experimental Area (39 to 184%). EBS-disturbed seafloor tracks contained 72 times more pyrosome-associated C than an undisturbed reference site, and up to 4 times more than an area disturbed in 1989. Deposited pyrosomes collected had a higher proportion of labile fatty acids compared to the sediment. We document the temporal and spatial extent of an abyssal food fall event with unprecedented detail and show that physical seafloor disturbance results in the accumulation of detrital material. Such accumulation may reduce oxygen availability and alter benthic community structure. Understanding both the relevance of large food falls and the fine scale topography of the seafloor, is necessary for impact assessment of technologies altering seafloor integrity (e.g. as a result of bottom-trawling or deep seabed mining) and may improve their management on a global scale.
Divide and conquer: genetics, mechanism, and evolution of the ferrous iron transporter Feo in Helicobacter pylori
Authors: Gómez-Garzón Camilo , Payne Shelley M. 
Published: July 2023

IntroductionFeo is the most widespread and conserved system for ferrous iron uptake in bacteria, and it is important for virulence in several gastrointestinal pathogens. However, its mechanism remains poorly understood. Hitherto, most studies regarding the Feo system were focused on Gammaproteobacterial models, which possess three feo genes (feoA, B, and C) clustered in an operon. We found that the human pathogen Helicobacter pylori possesses a unique arrangement of the feo genes, in which only feoA and feoB are present and encoded in distant loci. In this study, we examined the functional significance of this arrangement.MethodsRequirement and regulation of the individual H. pylori feo genes were assessed through in vivo assays and gene expression profiling. The evolutionary history of feo was inferred via phylogenetic reconstruction, and AlphaFold was used for predicting the FeoA-FeoB interaction.Results and DiscussionBoth feoA and feoB are required for Feo function, and feoB is likely subjected to tight regulation in response to iron and nickel by Fur and NikR, respectively. Also, we established that feoA is encoded in an operon that emerged in the common ancestor of most, but not all, helicobacters, and this resulted in feoA transcription being controlled by two independent promoters. The H. pylori Feo system offers a new model to understand ferrous iron transport in bacterial pathogens.
Functional outcome in low-ASPECTS (0–5) acute ischemic stroke treated with mechanical thrombectomy: impact of laterality explored in a single-center study
Authors: Dumas Victor , Martin Killian , Giraud Clément , Prigent Julia , Bloch William , Soualmi Karim , Herpe Guillaume , Boucebci Samy , Neau Jean Philippe , Guillevin Rémy , Velasco Stéphane 
Published: July 2023

BackgroundThere is no consensus regarding the influence of infarct laterality in patients with acute ischemic stroke due to anterior large vessel occlusion (AIS-LVO) treated with mechanical thrombectomy (MT), particularly in low-ASPECT (0–5) patients who were excluded from the initial MT studies and that participated in dedicated randomized-controlled trials that do not consider the side of the occlusion. We aimed to evaluate the role of infarct laterality on the clinical outcome in low-ASPECT AIS patients treated with MT.Material and methodsWe retrospectively analyzed our institutional stroke database in our Thrombectomy-Capable Stroke Center (TCSC), including patient characteristics, procedural variables, and outcomes, between January 2015 and January 2022. Patients with acute intracranial ICA and/or proximal MCA occlusions with ASPECT ≤ 5 either on CT or MRI were included and divided into 2 groups according to the location of ischemia. The primary endpoint was a good clinical outcome at 90 days (modified Rankin Scale (mRS) score of 0–3).ResultsBetween January 2015 and November 2021, 817 MT were performed, of which 82 were low-ASPECT (10.0%): 41 left-sided and 41 right-sided strokes. The rates of good clinical outcome were 30.8% (12/41) for the left-sided group and 43.6% (17/41) for the right-sided group, with a p-value of 0.349. The morality rate showed no significant difference between the two groups: 39.0% (16/41) in the right stroke group and 36.6% (15/41) in the left stroke group.ConclusionThe clinical outcome was not significantly influenced by stroke laterality. The results of this single-center retrospective study indicate either a lack of strength or equal value in performing mechanical thrombectomy regardless of stroke laterality.
The efficacy of a whole foods, plant-based dietary lifestyle intervention for the treatment of peripheral neuropathic pain in leprosy: a randomized control trial protocol
Authors: Klowak Michael , Boggild Andrea K. 
Published: July 2023

IntroductionDespite effective treatment of leprosy via WHO-approved multi-drug therapy (MDT), patients still suffer from debilitating neuropathic sequelae, including peripheral neuropathic pain (PNP), and continue to develop intercurrent etiologies (such as diabetes), and progressive existing neuropathy over time. Strategies seeking to improve physiological and metabolic wellness, including those that reduce systemic inflammation and enhance immune responsiveness to neurotoxic factors may influence underlying neuropathic etiologies. A whole food plant-based diet (WFPBD) has been shown to be effective in the management of neuropathic pain due to diabetes, limiting severity and relevant symptomology. Diabetes remains a significant sequela of leprosy, as up to 50% of patients in reaction requiring corticosteroids, may develop a biochemical diabetes. As nutritional interventions may modulate both leprosy and diabetes, a specific exploration of these relationships remains relevant.Objectives(1) To demonstrate the effect of a WFPBD lifestyle intervention, on neuropathic pain variables in leprosy; and (2) To contextualize the significance of diet in the treatment of chronic sequelae in leprosy by evaluating tolerability and side effect profile.MethodsA prospective, randomized, controlled, single-blind, multicentre interventional trial is described. Weekly one-hour dietary counseling sessions promoting a WFPBD emphasizing vegetables, fruits, whole-grains, nuts, and legumes, omitting animal products, and limiting fat intake over a six-month duration will be implemented. Participants will be 70 age and sex-matched individuals experiencing active or treated “cured” leprosy and PNP, randomized to either intervention or control groups. Primary outcome measures include efficacy via visual analog scale, subjective questionnaire and objective quantitative sensory testing, as well as safety, tolerability, and harms of a WFPBD on PNP in leprosy. This study will be initiated after Research Ethics Board (REB) approval at all participating sites, and in advance of study initiation, the trial will be registered atClinicalTrials.gov.Expected impactIt is hypothesized that WFPBDs will mitigate progression and severity of PNP and potentially reduce the adverse events related to standard corticosteroid treatment of leprosy reactions, thereby reducing disease severity. By examining the effects of WFPBDs on PNP in leprosy, we hope to illuminate data that will lead to the enhanced therapeutic management of this neglected tropical disease.
Effect of sedated colonoscopy with different cost coverage on improving compliance with colorectal cancer screening in China
Authors: Zhuo Lin , Kong Yunxin , Chen Siting , Ma Yue , Cai Ting , Pan Jianqiang , Wang Xiuying , Gao Yihuan , Lu Hang , Li Xinyue , Zhao Hongying , Mackay Louisa , Dong Wendi , Zhuo Lang , Dong Dong 
Published: July 2023

BackgroundColorectal cancer is the third most common cancer worldwide. Colonoscopy is the gold standard for colorectal cancer screening. However, the colonoscopy participation rate in China is much lower than that in Europe and the United States. As only non-sedated colonoscopies are offered in colorectal cancer screening programs in China, the absence of sedation may contribute to this gap.MethodsTo explore the effect of free and partially participant-paid sedated colonoscopy on improving colorectal screening participation, we conducted a cross-sectional study under the framework of the Cancer Screening Program in Urban China in Xuzhou from May 2017 to December 2020. The Quanshan district was set as the control group and provided free non-sedated colonoscopy, the Yunlong district was set as a partial cost coverage group and offered partially participant-paid sedated colonoscopy, and the Gulou district was set as the full cost coverage group and offered free sedation colonoscopies. Multivariate logistic regression was used for multivariate analysis of colonoscopy participation and colorectal lesion detection rates between the groups.ResultsFrom May 2017 to May 2020, 81,358 participants were recruited and completed questionnaire, 7,868 subjects who met high-risk conditions for CRC were invited to undergo colonoscopy. The colonoscopy participation rates in the control group, partially cost coverage, and full cost coverage groups were 17.33% (594/3,428), 25.66% (542/2,112), and 34.41% (801/2,328), respectively. Subjects in the partial and full cost coverage groups had 1.66-fold (95% CI: 1.48–1.86) and 2.49-fold (95% CI: 2.23–2.76) increased rates compared with those in the control group. The adjusted PARs for the partially and the full cost coverage group was 9.08 (95% CI: 6.88–11.28) and 18.97 (95% CI: 16.51–21.42), respectively. The detection rates of CAN in the control, partial-cost coverage, and full-cost coverage groups were 3.54% (21/594), 2.95% (16/542), and 5.12% (41/801), respectively. There were no significant differences in the detection rates between the group. However, sedated colonoscopy increases costs.ConclusionSedated colonoscopy increased colonoscopy participation rates in both the partial and full cost-covered groups. A partial cost coverage strategy may be a good way to increase colorectal cancer participation rates and quickly establish a colorectal cancer screening strategy in underfunded areas.
Bardet-Biedl syndrome caused by compound heterozygosity in BBS12 gene: a case report of one family with three affected members
Authors: Simičić Majce Ana , Tudor Darija , Simunovic Marko , Todorovic Marko , Parlov Mladenka , Lozic Bernarda , Saraga-Babić Mirna , Saraga Marijan , Arapović Adela 
Published: July 2023

IntroductionBardet-Biedl syndrome (BBS) is a rare genetic syndrome caused by a mutation in one of 26 different genes responsible for normal structure and/or function of primary cilia. The syndrome is characterized by multiorgan involvement with gradual onset of occurrence of clinical signs and symptoms resulting in great phenotypic variability and what is more important, often difficulties with establishing the timely diagnosis.Case reportWe report a case of a one family with three members with BBS caused by a very rare mutation, a compound heterozygosity in BB12 gene. Even though all three patients have the same type of mutation, they express a significant diversity in clinical expression as well as renal impairment.ConclusionThis is a case report of a rare clinical syndrome caused by a very rare genetic mutation and it emphasizes the importance of genetic analysis in the timely diagnosis of oligosymptomatic patients with BBS, in order to possibly prevent long-term complications.
Identification of the biological processes, immune cell landscape, and hub genes shared by acute anaphylaxis and ST-segment elevation myocardial infarction
Authors: Peng Zekun , Chen Hong , Wang Miao 
Published: July 2023

Background: Patients with anaphylaxis are at risk for ST-segment elevation myocardial infarction (STEMI). However, the pathological links between anaphylaxis and STEMI remain unclear. Here, we aimed to explore shared biological processes, immune effector cells, and hub genes of anaphylaxis and STEMI.Methods: Gene expression data for anaphylactic (GSE69063) and STEMI (GSE60993) patients with corresponding healthy controls were pooled from the Gene Expression Omnibus database. Differential expression analysis, enrichment analysis, and CIBERSORT were used to reveal transcriptomic signatures and immune infiltration profiles of anaphylaxis and STEMI, respectively. Based on common differentially expressed genes (DEGs), Gene Ontology analysis, cytoHubba algorithms, and correlation analyses were performed to identify biological processes, hub genes, and hub gene-related immune cells shared by anaphylaxis and STEMI. The robustness of hub genes was assessed in external anaphylactic (GSE47655) and STEMI (GSE61144) datasets. Furthermore, a murine model of anaphylaxis complicated STEMI was established to verify hub gene expressions. The logistic regression analysis was used to evaluate the diagnostic efficiency of hub genes.Results: 265 anaphylaxis-related DEGs were identified, which were associated with immune-inflammatory responses. 237 STEMI-related DEGs were screened, which were involved in innate immune response and myeloid leukocyte activation. M0 macrophages and dendritic cells were markedly higher in both anaphylactic and STEMI samples compared with healthy controls, while CD4+ naïve T cells and CD8+ T cells were significantly lower. Enrichment analysis of 33 common DEGs illustrated shared biological processes of anaphylaxis and STEMI, including cytokine-mediated signaling pathway, response to reactive oxygen species, and positive regulation of defense response. Six hub genes were identified, and their expression levels were positively correlated with M0 macrophage abundance and negatively correlated with CD4+ naïve T cell abundance. In external anaphylactic and STEMI samples, five hub genes (IL1R2, FOS, MMP9, DUSP1, CLEC4D) were confirmed to be markedly upregulated. Moreover, experimentally induced anaphylactic mice developed impaired heart function featuring STEMI and significantly increased expression of the five hub genes. DUSP1 and CLEC4D were screened as blood diagnostic biomarkers of anaphylaxis and STEMI based on the logistic regression analysis.Conclusion: Anaphylaxis and STEMI share the biological processes of inflammation and defense responses. Macrophages, dendritic cells, CD8+ T cells, and CD4+ naïve T cells constitute an immune cell population that acts in both anaphylaxis and STEMI. Hub genes (DUSP1 and CLEC4D) identified here provide candidate genes for diagnosis, prognosis, and therapeutic targeting of STEMI in anaphylactic patients.
Depression status and functional outcome of patients with ischemic stroke and the impact on caregivers living in Chengdu: a cross-sectional study
Authors: He Lanying , Wang Jian , Wang Feng , Wang Lu , Liu Yinglin , Zhou Fanfan , Xu Fan 
Published: July 2023

ObjectivesTo investigate the associations between risk factors and depression symptoms in ischemic stroke (IS) survivors and the effect of IS survivors’ depression status and functional outcomes on caregiver burden in Chengdu, China.MethodsIn this cross-sectional study, we recruited a convenience sample of patients with IS and paired caregivers living in Chengdu from February 2022 to May 2022. Depression symptoms were assessed using the 17-item Chinese Hamilton Depression Rating Scale, the social support of patients was assessed using the perceived social support scale (PSSS), caregiver burden was assessed using the Zarit burden interview (ZBI). Multivariable logistic regression analysis was used to analyze the data between risk factors and depression symptoms, and multiple linear regression models were constructed to examine the depression symptoms and functional outcomes of stroke survivors, and caregiver burden.ResultsIn total, 966 IS survivors and paired caregivers were included in this study. Among IS survivors, 35.51% (343/966) experienced depression. Age [adjusted odds ratio (aOR), 1.02; 95% confidence interval (CI), 1.00–1.04; p = 0.036], the National Institutes of Health Stroke Scale (NIHSS) score (aOR, 1.57; 95% CI, 1.47–1.68; p &lt; 0.001), and PSSS score (aOR, 0.86; 95% CI, 0.84–0.89; p &lt; 0.001) were associated with an increased risk of depression. The NIHSS score (b = 2.57, p &lt; 0.001), patients’ depression status (b = 2.54, p &lt; 0.001), duration of care (b = 0.359, p = 0.006), and social support of caregivers (b = −0.894, p = 0.038) were significantly associated with the ZBI score.ConclusionThe PSSS score was a major risk factor for the development of depression in IS survivors, and patients’ depression status and severe functional deficits had a negative impact on the ZBI score of the main caregivers. Social support can reduce the ZBI score.