bluuebunny/crossref_metadata_2025_split huggingface dataset:
Chemical conversion of human conventional PSCs to TSCs following transient naive gene activation
Authors: Zorzan Irene , Betto Riccardo Massimiliano , Rossignoli Giada , Arboit Mattia , Drusin Andrea , Corridori Clelia , Martini Paolo , Martello Graziano 
Published: February 2023

AbstractIn human embryos, naive pluripotent cells of the inner cell mass (ICM) generate epiblast, primitive endoderm and trophectoderm (TE) lineages, whence trophoblast cells derive. In vitro, naive pluripotent stem cells (PSCs) retain this potential and efficiently generate trophoblast stem cells (TSCs), while conventional PSCs form TSCs at low efficiency. Transient histone deacetylase and MEK inhibition combined with LIF stimulation is used to chemically reset conventional to naive PSCs. Here, we report that chemical resetting induces the expression of both naive and TSC markers and of placental imprinted genes. A modified chemical resetting protocol allows for the fast and efficient conversion of conventional PSCs into TSCs, entailing shutdown of pluripotency genes and full activation of the trophoblast master regulators, without induction of amnion markers. Chemical resetting generates a plastic intermediate state, characterised by co‐expression of naive and TSC markers, after which cells steer towards one of the two fates in response to the signalling environment. The efficiency and rapidity of our system will be useful to study cell fate transitions and to generate models of placental disorders.
BEKASAM: PANGAN TRADISIONAL YANG BERMANFAAT BAGI PASIEN HIPERTENSI
Authors: Lestari Yanesti Nuravianda , Fauzi Nur , Amin Nur 
Published: February 2023

Hipertensi masih menjadi masalah utama di dunia hingga saat ini. Banyak penelitian yang telah membuktikan bahwa hipertensi berhubungan dengan tingkat kesakitan dan kematian akibat penyakit kardiovaskular. Oleh sebab itu, penyakit hipertensi harus dicegah dan diobati, baik dengan obat-obatan maupun dengan modifikasi diet. Berdasarkan beberapa hasil penelitian, diketahui perlu adanya bahan makanan tertentu yang memiliki kemampuan antihipertensi dan dapat dimanfaatkan sebagai penyedap masakan yang aman bagi penderita hipertensi, salah satu yang dikaji yaitu bekasam. Bekasam merupakan produk hasil fermentasi spontan menggunakan kadar garam tinggi dengan bahan dasar ikan air tawar. Rasa dan aroma bekasam memiliki sangat khas dan dapat digunakan sebagai penyedap masakan. Bekasam diproduksi dengan mencampur ikan dengan nasi dan yang kemudian difermentasi selama 5-7 hari. Karakteristik daging bekasam sedikit kenyal dibandingkan dengan ikan segar, memiliki rasa asam asin khas bekasam dengan aroma tertentu. Berbagai penelitian menunjukkan bahwa bekasam ikan memiliki beberapa manfaat bagi kesehatan, antara lain aktivitas penghambatan Angiotensin-I-Converting Enzyme (ACE), yaitu enzim yang menyebabkan hipertensi dan juga sebagai penurun kolesterol karena mengandung lovastatin.
Uma bad trip pelo Brasil, com Richard Francis Burton
Authors: Dornelles Jonas Kunzler Moreira 
Published: February 2023

O trabalho procura revisitar a obra Explorations of the highlands of Brazil. de Richard Francis Burton, considerando o contexto do ano 2021. Nesse livro o viajante visita diversas regiões, registrando costumes, aspectos econômicos e geográficos, ao longo dos anos de 1865-1868. O itinerário começa pela atenção do autor para com as estruturas de transporte no Brasil. A seguir encontramos dois paralelos contemporâneos: o racismo e a mineração. Considerando sua trágica atualidade, podemos pensar o livro como um registro do apogeu do pensamento colonial, que segue presente de maneiras disfarçadas em certos clichês sobre a brasilidade que se repetem ainda nos dias de hoje. Para concluir, sugerimos que mesmo pelo caminho das descrições da natureza, o livro de Richard Burton pode nos trazer a sensação desesperada que muitos brasileiros sentem dentro do atual contexto de pandemia e retrocesso.
EVALUATION OF LIVER ELASTICITY USING pSWE AND 2D-SWE TECHNIQUES IN HEALTHY CHILDREN
Authors: AYDIN Hüseyin , İSSİ Fatma 
Published: February 2023

Objective
Shear wave elastography (SWE) is a new
ultrasonography technique that can non-invasively
evaluate liver stiffness. The efficacy of SWE in adults
has been demonstrated by numerous studies, but
there are few SWE studies of pediatric groups. Since
the liver stiffness values (LSV) vary according to the
device and the SWE method used, standard reference
values have not been established as yet. The aim of
this study was to establish the reference values of
normal liver stiffness in healthy children according to
the two SWE methods.
Materials and Method
Liver stiffness values were measured using the two
different SWE methods of point SWE (pSWE) and
2-dimensional SWE (2D-SWE) in 107 healthy children
aged 8-18 years with no liver disease. The pSWE
(EPQ) and 2D-SWE (EQI) measurements were taken
in one session on the same device (Philips Epiq Elite)
and after at least 4 hours of fasting. The effects on
liver elasticity values were investigated of gender,
age, body mass index (BMI), some biochemical
markers (such as fasting blood glucose, aspartate
aminotransferase (AST), alanine aminotransferase
(ALT), albumin, platelet, INR) and liver size.
Results
The mean LSV was 4.04±0.84kPa ((95% confidence
interval (CI):3.67-4.42)) on pSWE and 4.41±0.71kPa
(95% CI:4.09-4.72) on 2D-SWE. The upper limit LSVs
were found to be 4.42kPa and 4.72kPa, respectively.
The LSVs measured on 2D-SWE were found to be
higher than on pSWE ((median; min-max, 4.34kPa;
2.82-6kPa and 3.86kPa; 2.55–5.78, respectively,
(r=0.59, p
HPAF de <i>Xanthomonas axonopodis</i> PV. <i>manihotis</i> regula negativamente genes relacionados con metabolismo y defensa en hojas de yuca
Authors: Díaz-Tatis Paula A. , Trujillo-Beltrán César A. , Bernal-Giraldo Adriana J. , López-Carrascal Camilo E. 
Published: February 2023

Las bacterias fitopatógenas de los géneros Erwinia, Pantoea, Pseudomonas, Ralstonia y Xanthomonas causan una gran cantidad de enfermedades en diversos cultivos. La base molecular que explica parcialmente la patogenicidad de estas bacterias radica en la translocación de proteínas efectoras hacia el interior de las células hospederas a través del sistema de secreción tipo tres (SST3). Xanthomonas axonopodis pv. manihotis (Xam) es un bacilo gram negativo y es el agente causal de la bacteriosis vascular de la yuca (Manihot esculenta Crantz). Los estudios derivados de la secuenciación del genoma de diversas cepas de Xam han permitido la identificación de hrp-associated F (HpaF) como un efector principal presente en todas las cepas secuenciadas de Latinoamérica, África y Asia. En este trabajo se evaluó la importancia de HpaF en la virulencia de Xam empleando dos estrategias. Primeramente, se pudo determinar a nivel histológico cambios morfológicos en las células de yuca causadas por HpaF. En segundo lugar, se empleó una estrategia de transcriptómica comparativa empleando un microarreglo de ADNc de yuca y ARN obtenido de plantas de yuca inoculadas con cepas de Xam mutadas en hpaF (ΔhpaF) o complementadas (ΔhpaF +hpaF). Los datos obtenidos sugieren que HpaF es un factor de virulencia de Xam ya que regula negativamente genes involucrados en el metabolismo y defensa de la planta.
Self Card: Media Edukasi Untuk Peningkatan Rasa Percaya Diri Remaja Akhir
Authors: Hidayat Taufik , Istikomah Fatonah , Andriani Sekar Ayu Titis , Trilisiana Novi 
Published: February 2023

The stage of late adolescent development is the point at which a person searches for an identity that will greatly affect their future. This study aims to increase the self-confidence of late adolescents. Educational media innovations in the form of self card are made so that teenagers can carry out their daily activities with confidence. Confidence is specially designed to appear as content in the form of complete illustrations with words of suggestions that cover three aspects of self-confidence, namely self-ability, self-concept, and social interaction. The study was conducted using the Research and Development method with 9 subjects being students of the Faculty of Education, UNY, age19-20 years. Subjects selected based on the initial assessment had low self-esteem and were willing to voluntarily conduct a trial for 21 days. It can be concluded that this media is able to increase the self-confidence of late teens up to 18.25 percent. Qualitatively, through self-card interviews, they are able to give positive suggestions to late teens, so they can live the day with confidence.AbstrakTahap perkembangan remaja akhir adalah titik seseorang mencari jati diri yang akan sangat berpengaruh terhadap masa depannya. Penelitian ini bertujuan untuk meningkatkan rasa percaya diri remaja akhir. Inovasi media edukasi berupa self card adalah media edukasi yang dibuat agar remaja bisa menjalani aktivitas sehari-harinya dengan penuh percaya diri. Rasa percaya diri didesain khusus muncul menjadi konten berupa ilustrasi lengkap dengan kata-kata sugesti yang mencakup tiga aspek percaya diri yaitu kemampuan diri, konsep diri, dan interaksi sosial. Penelitian dilakukan menggunakan metode Research and Development dengan 9 orang subjek merupakan mahasiswa Fakultas Ilmu Pendidikan UNY berusia 19-20 tahun. Subjek dipilih berdasarkan asesmen awal yang memiliki rasa percaya diri rendah dan bersedia secara sukarela melakukan uji coba selama 21 hari. Penelitian ini melalui 6 tahapan yaitu, studi pendahuluan, pengumpulan data, penyusunan self card, validasi ahli dan revisi, uji coba self card dan revisi. Uji coba menggunakan metode pretest, midtest, dan posttest. Secara kuantitatif uji coba self card menunjukkan rata-rata pretest 64,3 persen, sedangkan rata-rata posttest 82,50 persen. Secara kuantitatif self card mampu meningkatkan rasa percaya diri remaja akhir hingga 18,25 persen. Secara kualitatif melalui wawancara self card mampu memberikan sugesti positif pada remaja akhir, sehingga dapat menjalani hari dengan percaya diri.
Estado del Arte de la Aplicación de las Normas GTC-ISO 26000, GTC-ISO 18091 y los Objetivos de Desarrollo Sostenible (ODS) en los Gobiernos Locales
Authors: Romero Pulido Jhoan Sebastián , Dworaczek Conde Helga Ofelia , Camacho Camacho Hernando , Aguilar Zacarías Ma. Lucila 
Published: February 2023

Entidades a nivel mundial se han visto inmersas en factores negativos en sus operativas económicas, administrativas, de producción y financieras por la deficiente ejecución de sus tareas, procedimientos y controles a cada uno de estos, conllevando a buscar alternativas que mitiguen el impacto a corto, mediano y largo plazo que satisfagan las exigencias del mercado actual sin exponer las competencias de las futuras generaciones, garantizando una ventaja competitiva en pro del logro estratégico propuesto en la Agenda 2030 por la Asamblea General de la ONU (ONU, 2021).
El presente artículo de investigación presenta como objeto, el diseño del estado del arte de la aplicabilidad de las normas ISO 26000, GTC-ISO 18091 y los Objetivos de Desarrollo Sostenible (ODS) a nivel nacional e internacional, que permitirá determinar las características estructurales, su uso, diferencias, similitudes y sus principales ventajas que los gobiernos locales pueden permitirse generar frente a una eficiente implementación, abordando las problemáticas principales y potenciales, a tal fin, de mejorar el negocio en sus diversas categorías.
Para la ejecución documental del estado del arte, se utilizó una metodología cualitativa documental de carácter descriptiva orientado a la búsqueda, compilación, observación, crítica e interpretación de fuentes primarias y secundarias, que parten de una revisión de literatura relacionada con la aplicación de las normas ISO 26000, ISO 18091 en los gobiernos locales y que de manera semejante se realizó la exploración de los objetivos de desarrollo sostenible abordando aspectos económicos, ambientales y sociales.
Los resultados del análisis de la información reflejaron que las normas ISO permiten que los gobiernos locales funcionen de manera óptima, asegurando productos y/o servicios con los más altos modelos de calidad, satisfaciendo las necesidades y expectativas demandados por los consumidores y/o clientes finales, que ayudaran al cumplimiento de los objetivos de desarrollo sostenible (ODS).
Assessing Self-Reported Readiness of Medical Students transitioning to Clinical Clerkship at the University of Ottawa
Authors: Mistry Neel , De Laplante Stefan , Murray Campbell Craig 
Published: February 2023

Introduction: The transition from pre-clerkship to clinical clerkship is a pivotal moment for medical students. Curricular improvements can be made to better prepare students for clerkship. We collected student feedback to generate recommendations for improvement with regard to clerkship preparedness at the University of Ottawa Faculty of Medicine.
Methods: We created a pre- and post-clerkship transition survey for medical students at the University of Ottawa Faculty of Medicine. The groups assessed were from different cohorts. Likert-type and open ended questions were used. The survey was open from October 10 to October 31, 2020. Microsoft Excel 2016 was used for data analysis.
Results: We obtained 176 respondents (37% response rate), of which 158 provided consent and completed the survey. Students in the post-transition group were less anxious about the transition to clerkship, compared to their pre-transition colleagues, with the most significant difference being completing a thorough history and physical examination (2.9/5.0 vs. 3.3/5.0, p<0.05). The two main stressors for incoming clerks were inadequate clinical skills training in pre-clerkship and lack of clarity around clerkship roles, responsibilities, and expectations.
Conclusion: Improvements can be made in pre-clerkship through the integration of small-group orientation sessions, formative OSCEs, accelerated review of pre-clerkship material, and clerkship simulation sessions to facilitate a seamless transition to clerkship at the University of Ottawa.
Prospective study of platelet count as a prognostic marker in predicting feto-maternal outcome in gestational hypertension
Authors: Saisree M. Jyothsna , Alluri Shreya , Kameswaramma K. 
Published: February 2023

Background: Hypertensive disorders of pregnancy are one of the leading causes of maternal and neonatal morbidity and mortality worldwide. Hypertensive disorders of pregnancy (HDP) affects approximately 5-7% of all pregnancies. The reported incidence of HDP in India ranges from 5% to 15%. These disorders form a deadly triad-in conjunction with hemorrhage and infection, in significantly contributing to maternal morbidity and mortality. Objectives were to determine the correlation between the severity of HDP and low platelet count; to analyze maternal and fetal outcomes in relation to thrombocytopenia; to aid in early diagnosis and management and to prevent complications and thereby improving maternal and fetal outcomes.
Methods: A hospital-based prospective study was conducted in women with a provisional diagnosis of gestational hypertension over a period of 18 months. The study group comprised of pregnant women who were more than 20 weeks and were subjected to detailed history: pre-obstetric history, family history, general physical examination, abdominal examination, routine laboratory investigations, and ultrasonography and Doppler.
Results: In the study, platelet count at ≤275000 cut-offs had the highest sensitivity of 52.11%, specificity of 82.76%, PPV of 88.1%, and NPV of 41.4% in predicting feto maternal complications. Platelet count at ≤275000 cut-offs had the highest sensitivity of 62.86%, specificity of 69.23%, PPV of 52.4%, and NPV of 77.6% in predicting preeclampsia.
Conclusions: From the study, it was concluded that in gestational hypertension the estimation of platelet count is thus a reliable method for early detection and management of hypertensive disorders of pregnancy. Platelet count was significantly decreased in subjects with maternal complications, fetal complications, and with respect to the severity of preeclampsia. Platelet count however had moderate validity in predicting fetomaternal complications.
Pelaksanaan Fungsi Pengawasan Dinas Lingkungan Hidup Kabupaten Pacitan Terhadap Pengelolaan Limbah B3 PLTU Sudimoro Di Kabupaten Pacitan
Authors: Pradana Oky Shiang , Handayani I Gusti Ayu Ketut Rachmi 
Published: February 2023

<p><em>This legal writing has the purpose to knowing the supervision by the Pacitan District Enviromental Services on Hazardous Waste Management at the PLTU Sudimoro in Pacitan Regency. This legal research is included in the type of empirical legal research that has character is descriptive and uses a qualitative approach. In this study the source of the data obtained is the primary data source, which is based on the results of direct interviews with related sources, namely the Pacitan District Enviromental Services. Apart from primary data, it is also based on secondary data with library research.</em></p><p><em>Based on the results of the research that has been carried out, it can be concluded that the Environmental Service of Pacitan Regency in exercising its supervisory authority for hazardous waste management has run quite well and is in accordance with the applicable laws and regulations. In the supervision of the B3 waste management, PLTU Sudimoro that has been carried out has covered all the stages related to the supervision of hazardous waste management at the district / city level. However, in exercising the authority to supervise the management of B3 waste from PLTU Sudimoro by the Environmental Service of Pacitan Regency, several inhibiting factors were found, namely related to human resources, activity budget, geographical conditions, and limited operational vehicles.</em></p>
Influence of Scan Parameters of Single and Dual-Energy CT Protocols in Combination with Metal Artifact Suppression Algorithms for THA
Authors: Schwarz Gilbert M. , Huber Stephanie , Wassipaul Christian , Kasparek Maximilian , Hirtler Lena , Hofstaetter Jochen G. , Bader Till , Ringl Helmut 
Published: February 2023

Background:
Metal artifacts caused by hip arthroplasty stems limit the diagnostic value of computed tomography (CT) in the evaluation of periprosthetic fractures or implant loosening. The aim of this ex vivo study was to evaluate the influence of different scan parameters and metal artifact algorithms on image quality in the presence of hip stems.

Methods:
Nine femoral stems, 6 uncemented and 3 cemented, that had been implanted in subjects during their lifetimes were exarticulated and investigated after death and anatomical body donation. Twelve CT protocols consisting of single-energy (SE) and single-source consecutive dual-energy (DE) scans with and without an iterative metal artifact reduction algorithm (iMAR; Siemens Healthineers) and/or monoenergetic reconstructions were compared. Streak and blooming artifacts as well as subjective image quality were evaluated for each protocol.

Results:
Metal artifact reduction with iMAR significantly reduced the streak artifacts in all investigated protocols (p = 0.001 to 0.01). The best subjective image quality was observed for the SE protocol with a tin filter and iMAR. The least streak artifacts were observed for monoenergetic reconstructions of 110, 160, and 190 keV with iMAR (standard deviation of the Hounsfield units: 151.1, 143.7, 144.4) as well as the SE protocol with a tin filter and iMAR (163.5). The smallest virtual growth was seen for the SE with a tin filter and without iMAR (4.40 mm) and the monoenergetic reconstruction of 190 keV without iMAR (4.67 mm).

Conclusions:
This study strongly suggests that metal artifact reduction algorithms (e.g., iMAR) should be used in clinical practice for imaging of the bone-implant interface of prostheses with either an uncemented or cemented femoral stem. Among the iMAR protocols, the SE protocol with 140 kV and a tin filter produced the best subjective image quality. Furthermore, this protocol and DE monoenergetic reconstructions of 160 and 190 keV with iMAR achieved the lowest levels of streak and blooming artifacts.

Level of Evidence:
Diagnostic Level III. See Instructions for Authors for a complete description of levels of evidence.
NEOICHNOLOGY OF ARMADILLOS: KEYS FOR THE PALEOECOLOGICAL AND BEHAVIORAL INTERPRETATION OF FOSSIL TETRAPOD BURROWS
Authors: CARDONATTO M. CRISTINA , MELCHOR RICARDO NÉSTOR 
Published: February 2023

ABSTRACTThis study is based on the analysis of burrow casts of three species of armadillos from central Argentina: Chaetophractus villosus, Chaetophractus vellerosus, and Zaedyus pichiy (Chlamyphoridae: Euphractinae). The aim of this work was to identify key ichnologic signatures of Euphractinae armadillo burrows for application to the paleoecological and behavioral interpretation of fossil examples. A total of 15 active burrows from different biogeographic provinces were casted with polyurethane foam and then excavated. About two hundred uncasted burrows and foraging pits were also measured at the same localities. Euphractinae burrows are clearly distinguished from Chlamyphorinae burrows by its filling, surface ornamentation, and size. It is suggested that fossil armadillo (Euphractinae) burrows would be characterized by a single ramp with one entrance and massive or laminated fill, horizontal diameter larger than 100 mm, strongly marked sets of three claw traces that are arranged oblique to the ramp axis, and absence of feces or plant remains. The distinctive surface ornamentation present in the casted burrows is tentatively linked to a particular excavation mechanism involving rotation of the body along the antero-posterior axis. Burrow systems with a chamber are interpreted as permanent burrows, whereas those lacking chamber are considered as temporary/shelter burrows. Armadillo burrow systems (either temporary or permanent) are longer, less inclined, and have lower relative diameter indices (RDI) than armadillo foraging pits. Euphractinae burrows are linked to producers with solitary, fossorial, opportunistic omnivorous habits that excavate several burrows during their lives. They are left open after abandonment, and preferably located in xeric shrub lands and grasslands. The described morphological features of the burrows systems can be useful for the interpretation of fossil (post-Eocene) burrows.