bluuebunny/crossref_metadata_2025_split huggingface dataset:
Cerebrospinal Fluid (CSF) Proteomic Signature in Preclinical and Clinical AD: Role of Adhesion Molecules.
Authors: Hajjar Ihab , Neal Reem , Yang Zhiyi , Obideen Malik , Peterson Melissa , Shah Amil 
Published: January 2025

Abstract

Background: Although Amyloid-beta and Tau are the hallmarks of Alzheimer’s Disease (AD), other protein pathways such as endothelial dysfunction may be involved and may precede cognitive symptoms. Our objective was to characterize the cerebrospinal fluid (CSF) proteomic profiles focusing on cardiometabolic-related protein pathways in individuals on the AD spectrum.
Methods: We performed CSF and plasma-targeted proteomics (276 proteins) from 354 participants of the Brain Stress Hypertension and Aging Program (BSHARP), of which 8% had preclinical AD, and 24% had MCI due to AD. We instituted a bioinformatic pipeline to generate data-driven protein modules, used “Hub” and "Critical” proteins within each module to describe protein signatures for each AD stage and then assessed their associations with clinical and biological AD traits. Finally, we completed pathway enrichment analysis to get insight into pathways that might be implicated in AD pathogenesis.
Results: The 276 measured proteins clustered into five modules that were associated with CSF Amyloid-β42, Tau, and pTau. (all p-value <0.05). A CSF protein AD signature was characterized by elevated levels of CSF Hepatocyte Growth Factor (HGF), Intercellular and Vascular Cell Adhesion Molecule 1 (ICAM-1, VCAM-1), Neuropilin 1 and 2 (NRP-1, NRP-2), Scavenger Receptor Class B Member 2(SCARB2), Plasminogen Activator, Urokinase (PLAU). (all <0.05) We also found a significant difference in the CSF/Plasma ratio for the proteins associated with Cognitive Status and the Tau/Aβ42 ratio (TAR) in the CSF. Pathway enrichment analysis revealed that cell adhesion and endothelial dysfuncton(all p-value <0.05) were key mechanisms involved in AD pathogenesis, especially in the preclinical stage.
Conclusion: Our results suggest a proteomic signature in the CSF of individuals with preclinical AD that is driven by adhesion molecules and might be implicated in the pathogenesis of AD. Future studies investigating these pathways may provide insights into novel AD biomarkers and therapeutic targets.
Effect of Storage Conditions on Content of 32 Pesticide Residues in Detox Waters
Authors: kıralan sezer , Gökduman Saliha 
Published: January 2025

Abstract

Detox water, a beverage widely consumed for weight management and health benefits, is typically prepared using fruits and vegetables such as green apple, parsley, cucumber, mint, and lemon. This study evaluated the impact of storage conditions (ambient temperature and 4°C) over 72 hours on the concentrations of 32 pesticide residues commonly found in these ingredients. Changes in pesticide residue levels and their removal rates were analyzed during storage. Results demonstrated a consistent decrease in pesticide residue concentrations with longer storage durations under both conditions. Notably, at ambient temperature, the pesticide Clofentezine exhibited the highest removal rate of 89% after 72 hours. These findings provide valuable insights into the behavior of pesticide residues in aqueous media under varying storage conditions, contributing to improved safety practices for detox water preparation and storage.
Atonik and DA-6 enriched nano Silicon fertilizer for growth and phytochemical, and mineral uptake properties enhancement in Zataria multiflora Boiss
Authors: Mostafavi Sahar , Gharneh Hoseinali Asadi , Tavallali Vahid , Rowshan Vahid 
Published: January 2025

Abstract

The goal of this study was to identify the growth, phytochemical composition, and nutrient uptake of Zataria multiflora using various silicon-based treatments (sodium silicate, DA-6, Atonik and green-synthesized silica-DA6, and silica-Atonik). Three concentrations (1.5, 3, and 6 mM) of each treatment were foliar-applied to thyme seedlings in a randomized complete block design. DA-6 and its nano-silicon complex (nSi-DA6) at 3 and 6 mM significantly increased shoot length and root fresh weight. DA-6 also boosted the inflorescence's fresh weight. Essential oil content increased slightly with several treatments, reaching a maximum of 3.21% with 6 mM nSi-DA6. nSi-Atonik and nSi-DA6 treatments yielded the highest levels of epicatechin and naringenin, while silica nanoparticles, nSi-Atonik, and nSi-DA6 maximized rosmarinic acid content. Silicon content increased in treated plants, but zinc and nitrogen levels remained unaffected. Enhanced growth in silica-treated plants is attributed to improved photosynthesis, transpiration, chlorophyll content, and enzyme activity. Overall, the study demonstrates that silicon-nanocomplexes treatments can positively influence Zataria multiflora growth and phytochemical production, with nSi-DA6 and nSi-Atonik showing particularly promising results.
Hydraulic Performance Optimization of Airfoil Weir-Orifice Facilities Based on Improved Hicks-Henne Shape Function and MOPSO Algorithm
Authors: Sun Bin , Liu Xiangyang , Hu Lianghan , Zhao Hangbing , LI Zhiwei , Wang Feifei 
Published: January 2025

Abstract

In order to better measure and control the flow rate in open channel systems, this study proposes a hydraulic performance optimization system for airfoil weir facilities. The optimization system is built around three essential modules: the modified Hicks-Henne shape function, CFD numerical simulation, and the Multi-Objective Particle Swarm Optimization (MOPSO) algorithm, which are central to airfoil shape reconstruction. In a specific example with a flow rate of 0.033 m³/s and a rotation angle of 15°, optimization concepts for head loss and submergence were applied. The results show that the optimized design reduced head loss by 9.14% and increased submergence by 5.99%. Moreover, the optimized design demonstrated excellent hydraulic performance under different angles and flow conditions. To further validate the optimization effect, the flow rate formula was derived using the π-theorem in dimensional analysis and the theory of incomplete similarity. The results indicate that the optimized airfoil weir facility’s flow rate formula is more accurate with significantly reduced errors. This study provides strong theoretical and practical support for the design and optimization of similar hydraulic structures.
EtSERPIN1 binding with chicken ANXA2 is essential for Eimeria tenella attachment and invasion process
Authors: Wang Zengbao , Li Taifeng , Jiang Yingying , Wang Xue , Li Hongmei , Zhao Xiaomin , Zhang Xiao , Zhao Ningning 
Published: January 2025

Abstract

Serpin protease inhibitors (SERPINs) in protozoa play crucial roles in various biological processes, including the invasion of host cells. However, the precise roles and molecular mechanisms underlying SERPIN-mediated invasion of parasite remain poorly understand. In this study, we provide evidence that surface-expressed Eimeria tenella SERPIN1 (EtSERPIN1) on sporozoites is involved in adhesion and invasion processes. To elucidate the molecular target responsible for mediating EtSERPIN1-induced invasion, we utilized GST pull-down and yeast two-hybrid verification to screen and identify host cell membrane proteins interacting with EtSERPIN1. Our findings revealed an interaction between EtSERPIN1 and a membrane protein called annexin A2 (ANXA2). Recombinant GgANXA2 was able to bind to the sporozoite surface. Furthermore, treatment with GgANXA2-specific antibody or recombinant GgANXA2 protein resulted in a dose-dependent inhibition of EtSERPIN1 binding to host cells as well as sporozoite invasion. These results suggest that EtSERPIN1 and GgANXA2 interaction plays a critical role in both adhesion and invasion processes of E. tenella sporozoites. Finally, we investigated the impact of recombinant GgANXA2 and EtSERPIN1 proteins on E. tenellainfection. Our results demonstrated that incubation with GgANXA2 protein significantly attenuated sporozoite infectivity, as evidenced by a significantly reduction in parasite burden within the chicken cecum. Immunization with recombinant EtSERPIN1 exhibited potent anti-E. tenella activity, with higher body weight gains, lower cecal lesions and oocyst output, as well as elevated levels of cecal mucosa antibodies. These findings suggest that targeting GgANXA2 through EtSERPIN1 mediates adhesion and invasion processes of E. tenella, highlighting its potential as a novel therapeutic target.
Mutational analysis of the role of conserved histidine residues of the SDHC subunit of Complex II
Authors: Yadava Nagendra 
Published: January 2025

Abstract

Background: SDHC protein is a critical component of the succinate-ubiquinone oxidoreductase also known as respiratory chain Complex II. Because SDHC mutations are associated with several human diseases, a detailed understanding of the role of SDHC in Complex II and overall cellular physiology is important. In this study, we have tested the functional complementation of SDHC-null cells with different variants of the SDHC proteins fused with GFP at the C-terminus. Specifically, we determined the role of four conserved histidines (H55, H71, H127, H134) in SDHC on Complex II function and mitochondrial morphology.
Methods: The SDHC-deficient Chinese hamster cell line CCL16-B9 was transfected with the plasmid constructs expressing the SDHC variants (SDHC, SDHC∆, SDHC-H55N, SDHC-H71N, SDHC-H127N, SDHC-H134N) fused with either GFP or HA-GFP at the C-terminus. Restoration of Complex II function in the transfected CCL16-B9 cells by the SDHC variants was tested by galactose selection, biochemical assays, Western blotting, and the assessment of mitochondrial morphology by fluorescence microscopy.
Results: The key findings of this study are: (1) The GFP at the C-terminus does not interfere with SDHC import, orientation, and function. (2) Wild type and mutant SDHCs can deliver GFP or HA-GFP into the IMS. (3) The third transmembrane domain is essential for SDHC function. (4) While H55, and H71are non-essential, the H127 and H134 are essential for SDHC function. The H127N and H134N mutations impair Complex II function and alter mitochondrial morphology. The most detrimental effects are due to H127N mutation. (5) SDHC can serve as a carrier for delivering the proteins of interest into IMS.
Conclusions: This study advances our understanding of the role of SDHC subunit in Complex II, mitochondrial morphology, and the overall cellular physiology.
Dynamics of ACT parasite clearance in falciparum malaria among a cohort of inhabitants in a malaria hyper-endemic focus, Ilorin Nigeria
Authors: Shittu Olalere , Obimakinde Ebenezer Tolulope , Oladosu Samuel Ololade , Abdulkodri Mubarak Damilola 
Published: January 2025

Abstract

Background
An appreciable reduction in parasite clearance time (PCT) following artemisinin combination therapy (ACT) is a strong prognosis for resistance, which hitherto threatens the global malaria elimination. The study exposits the accurate measurement of PCT following clinical ACT regimen among inhabitants in a malaria hyper-endemic focus, Ilorin, Nigeria.
Methods
Malaria-positive in-patients were periodically administered ACT and their parasitaemia was determined microscopically at 0, 12, 24, 36 and 72 hours. The data obtained were uploaded on the world wide antimalarial resistant network (WWARN).
Results
One hundred and twenty-one (121) valid malaria cases were evaluated using established algorithm. The following ensued, viz; lag-phase (10); median positive parasite slide (0.09) per patient; range (0.04–0.10); interquartile range (0.08–0.09); clearance rate/hour constant (K, 1/hour); distribution (N = 3, 2.48%, 0-0.05) and (N = 118, 97.52%, 0.05–0.10) respectively. The slope half-life median (range; IQR) of 7.96 (7.22–15.47; 7.74–8.42) hour. Parasite clearance was as follows; 50% (13.30; 11.86–16.78; 13.21–13.34), 90% (32.00; 30.75–47.79; 31.53–33.08), 95% (40.06; 38.23–63.26; 39.38–41.19) and 99% (58.20; 55.59–99.19; 57.55–60.74) per hour of initial value. The minus slope of the Tobit regression revealed three models, viz; best fit at 50hours (Type I); 30hours (Type II) and 20hours (Type III).
Conclusion
The dynamics with delayed parasite clearance does not entirely reflect ACT failure, but it is pertinent to monitor other early signs of resistance. Artemisinin derivatives still remain the drug of choice because of its half-life and the potentials for eliminating early ring stage.
Local Microglial Activation Induced and Labeled in the Retina in a Novel Subretinal Hemorrhage Mouse Model
Authors: Balogh Boglárka , Zille Marietta , Szarka Gergely , Péntek Loretta , Futácsi Anett , Völgyi Béla , Kovács-Öller Tamás 
Published: January 2025

Abstract

Subretinal hemorrhage (SRH) is caused by the accumulation of blood between the neurosensory retina and the retinal pigment epithelium or between the retinal pigment epithelium and the choroid. It often arises from age-related macular degeneration, traumas, and may occur spontaneously caused by other diseases like hypertension and diabetes. Here, we developed a novel technique by co-injection of blood and a dye-coupled tracer protein, Cholera toxin subunit B (CtB), to better localize and understand the disease and how it can cause microglial activation, inflammation, and partial vision loss. Our results show that microglia are activated in the inner retinal regions and also in the zones neighboring the blood injection. In contrast, the non-affected zone of the affected eye showed no microglial activation. Based on the results, we provide evidence of inner retinal microglial activation and the appearance of microglia and macrophages in the pigment epithelium. Using advanced imaging techniques, we were able to localize better the affected area that comprises not only the retinal area over the blood clot but the neighboring regions as well. These findings will provide the basis for novel therapeutic interventions targeting neuroinflammation in the retina after subretinal hemorrhage and other diseases affecting the eye.
First record of Ixodes keiransi (Acari: Ixodidae) ingestion by Peromyscus yucatanicus (Rodentia: Cricetidae)
Authors: Torres-Castro Marco , Suárez-Galaz Alejandro , Panti-May Jesús Alonso , Sánchez-Montes Sokani , Yam-Lozada Andrea , Yeh-Gorocica Aarón , Reyes-Novelo Enrique , Colunga-Salas Pablo , Aguilar-Tipacamú Gabriela , Rodríguez-Vivas Roger Iván 
Published: January 2025

Abstract

This paper aims to report the ingestion of Ixodes keiransi by Peromyscus yucatanicus. Fragments of two ticks (larvae) were collected from the intestinal content of a P. yucatanicus captured in Yucatan, Mexico. DNA was obtained from both specimens, and a fragment of 400 bp of the 16S rRNA gene was amplified and sequenced. Bioinformatics analyses were performed, and a phylogenetic tree was constructed to determine the identity of the collected ticks. Peromyscus yucatanicus is a wild rodent endemic to the Yucatan Peninsula. It carries vector-borne pathogens and the arthropods that transmit them. Its diet consists of different elements, including arthropods. However, this is the first report of this rodent's ingestion of Ixodes keiransi. This tick is a biological vector of important public health pathogens, including Rickettsia and Borrelia. This finding opens questions and hypotheses about alternative enzootic transmission mechanisms of tick-borne pathogens through tick ingestion in endemic areas.
The gain-of-function mutation of the rice auxin-signaling repressor, OsIAA13, induces rice bacterial blight resistance through activating jasmonic acid-mediating defense system
Authors: Suzuki Go , Murakami Aina , Moriyasu Yutaro , Fukuda Manatsu , Uji Yuya , Onohata Tomonori , Fujii Yumi , Inukai Yoshiaki , Gomi Kenji 
Published: January 2025

Abstract

Jasmonic acid (JA) is involved in the regulation of rice defense responses against Xanthomonas oryzae pv. oryzae (Xoo). JA also affects other plant hormone signaling to maximize the JA-induced defense responses in rice. In this study, we investigated the JA- and auxin-mediating defense system using a gain-of-function mutant of OsIAA13, a suppressor of auxin signaling, in rice. The expression of some auxin-responsive expansins was downregulated in the Osiaa13 mutants. The Osiaa13 mutants showed a JA-hypersensitive phenotype. The expression of some JA-responsive defense-related genes such as the lignin biosynthesis gene, OsPrx38, was upregulated in the Osiaa13 mutants. Lignin content was higher in the Osiaa13 mutants than in the wild type plants. The expression of OsPrx38 was downregulated after IAA treatment. Furthermore, some antibacterial compounds against Xoo accumulated in the Osiaa13 mutants. These results suggest that OsIAA13 has an important role in disease resistance against Xoo by regulating JA- and auxin-mediating defense system in rice.
Acceptability, feasibility, fidelity and quality implementation of the culturally adapted version of The Social Competence Promotion Program among Young Adolescents (“Mi Mejor Plan”) to prevent substance use among adolescents in Chile: a pilot randomized control study
Authors: Ramirez Saray , Ríos Natalia , Rojas-Barahona Cristian A. , Cárcamo Marcela , Sepúlveda Alejandro , Araya Ricardo , Gaete Jorge 
Published: January 2025

Abstract

Introduction. Substance use among adolescents is a public health problem. We culturally adapted The Social Competence Promotion Program for Young Adolescents (SCPP-YA) program to the school context in Chile (henceforth “Mi Mejor Plan or MMP”) and assessed the acceptability, feasibility, fidelity, and quality of the implementation among 6th graders. We also explored the efficacy of the program in improving individual protective factors and reducing risk factors and substance use.
Methods. Cluster randomized controlled trial conducted in Chile. The schools were randomly assigned to one of two conditions in a 1:1 ratio: 1) the "MMP" intervention group, and 2) the Control group. The program consisted of a 16-hour class-based curriculum promoting social problem-solving skills delivered by a trained facilitator. Primary outcomes were acceptability, feasibility, fidelity, and quality of the implementation using detailed reports of facilitators and from observers of the performance of the facilitators in vivo. Additionally, we explored the efficacy of the intervention on secondary outcomes: 30-day prevalence of tobacco, alcohol, and cannabis use and individual risk and protective factors promoted by MMP. We performed an intention-to-treat analysis using mixed models, taking into account the hierarchical nature of the data.
Results. 765 6th graders from 11 schools were enrolled (one school dropped out after the randomization); 608 were analyzed at baseline, and 538 were analyzed post-intervention. 52.5% were male, and the average age was 11.3 in both groups. All 16 sessions were implemented, and students’ attendance at each session ranged from 83.8% to 92.4%. The program was generally well-received, with up to 91.3% of students rating acceptability positively. Facilitators and observers reported high adherence to the contents of the program in most sessions. Protective factors, such as negative beliefs about tobacco and alcohol, increased significantly in the IG. Still, there were no significant changes in substance use, risk factors, emotion regulation, or school membership.
Conclusions. The MMP program was well accepted, and we achieved high levels of implementation and fidelity. The program improved some individual protective factors, such as negative beliefs about tobacco and alcohol, with changes in substance use among adolescents.
Trial registration. Registered on ClinicalTrials.gov, number NCT04236947.
A New Multilayer Takagi Sugeno Kang Elliptic Type- 2 Fuzzy Cerebellar-Imitated Neural Network for Nonlinear Systems Using Modified Grey-Wolf Algorithm
Authors: Huynh Tuan-Tu 
Published: January 2025

Abstract

This paper aims to develop a new efficient multilayer neural network structure by combining the elliptic type-2 membership function with a Takagi- Sugenno-Kang (TSK) fuzzy system and a cerebellar model articulation controller to create a new multilayer TSK elliptic type-2 fuzzy cerebellar-imitated neural network (MTET2FCNN). The MTET2FCNN can be used as the main controller in the control problem, and it is also respectively used as the main identifier and predictor for identification and prediction problems. In addition, a modified Grey-Wolf optimization algorithm is used to update the optimal learning rates of the MTET2FCNN structure. System parameter learning is performed for all rules of the proposed MTET2FCNN structure based on the gradient descent algorithm and cost function minimization. The robust design of the compensator and the system's stability are proved by Lyapunov theory. The synchronization of a chaotic 4D Rabinovich system, the identification of a time-varying system, and a Mackey-Glass time series prediction demonstrate the performance and effectiveness of the proposed system.
Is green metallic sheen in emb agar accurate to assist the Escherichia coli identification?
Authors: Souza Hosana Dau Ferreira de , Ferreira Paula Fernanda Alves , Patricio Thereza Cristina da Costa , Santiago Gabrielli Stefaninni , Coelho Irene da Silva , Souza Miliane Moreira Soares de , Fernandes Kayo Cesar Bianco , Coelho Shana de Mattos de Oliveira 
Published: January 2025

Escherichia coli is an essential bacteria as a human and animal pathogen, although this species can also be disseminated in the environment. The clinical laboratories use EMB agar (Eosin Methylene Blue) to identify E. coli from the observation of metallic green sheen. However, other bacterial species may show sheen when sown on EMB agar, or in some cases, the pH sample influences the presence of this sheen by E. coli. In this study, MALDI-TOF was used as the gold standard to detect E. coli from human, animal, and environmental samples by analyzing the presence of green glow on EMB agar. The positive predictive values (PPV) ​​were higher (above 85%) in human, animal, and environmental samples studied, and the negative predictive value (NPV) was variable between the samples. Specificity and sensitivity were also variables suggesting the influence of the pH of the samples in the presence of metallic green brightness. This work demonstrated that it is necessary to implement biochemical tests or, when possible, molecular assays to confirm E. coli since only the observation of the production of metallic green glow in EMB can generate false negative results in the identification of E. coli.
Keywords: Biochemical tests; Escherichia coli; Laboratory Analysis; MALDI-TOF.