bluuebunny/crossref_metadata_2025_split huggingface dataset:
Examining the Economic Costs of the 2003 Halloween Storm Effects on the North Hemisphere Aviation Using Flight Data in 2019
Authors: Xue Dabin , Yang Jian , Liu Zhizhao , Yu Shiwei 
Published: March 2023

AbstractSpace weather can impede normal aviation operations through communication blackouts, GNSS‐based navigation and surveillance failures, and elevated cosmic radiation, consequently resulting in necessary flight plan adjustments and considerable economic costs. Although space weather effects have been heavily emphasized, the literature on the economic effects on aviation is limited. In this study, we estimate the economic impacts from the perspective of air traffic management, assuming an extremely strong space weather event like the 2003 Halloween solar storm would occur in 2019 with a booming air transport industry in recent years. We find that (a) as the high‐frequency communication blackouts may lead to polar flight rerouting and cancellations, possible daily economic costs could range from €0.21 million to €2.20 million per day; (b) during the satellite navigation failure period in the continental United States, as aircraft utilizes ground navigation aids as a backup, the increased flying time and disrupted descent approach operations may lead to additional cost of €2.43 million; (c) a surveillance failure can reduce airspace capacity and increase the workload of air traffic controllers, resulting in fatigue and perhaps risking flight safety; (d) to prevent massive cosmic radiation exposure, the economic costs of flight cancellations can be from €2.77 million to €48.97 million, depending on the cosmic radiation dose limits for a given plan. Our study indicates that severe space weather events may briefly disrupt normal aviation operations and cause substantial economic losses if future aviation equipment and technology are fragile to its effects.
Formation of the Layered Circulation in South China Sea With the Mixing Stimulated Exchanging Current Through Luzon Strait
Authors: Cai Zhongya , Chen Dake , Gan Jianping 
Published: March 2023

AbstractUsing the layered circulation in the South China Sea (SCS) as an example, a process‐oriented simulation was conducted to explore how the lateral exchanging current and basin circulation responded to the contrasting mixing in the marginal sea‐open ocean system and to the internal modulation over basin slope. Under conditions of intensified turbulent mixing () in the SCS, a crossing Luzon Strait density difference was generated and the net ageostrophic pressure gradient force drove the layered exchanging current with intensified westward deep intrusion into the SCS and an eastward middle outflow. The lateral exchanging current in turn developed a layered basin circulation in the semi‐enclosed middle and deep layers through directly providing external planetary vorticity flux, and releasing the internal potential energy indirectly. The vertical migration of the layered circulation structure over bottom slope owing to flow‐topography interaction is the major intrinsic response to the external exchanging current particularly in the semi‐enclosed deep basin. Although a small was adequate to maintain the basic layered circulation pattern, an intensified was required to extend the deep cyclonic circulation to the bottom. The intrusion of the western boundary current from open ocean not only maintained the strong upper cyclonic circulation but also intensified deep intrusion and modulated the deep layer circulation through vertical coupling. Both the deep density difference between the SCS and open ocean as well as associated vertical coupling should be considered to well understand the intensity of water exchange and the consequent layered circulation in the SCS.
MALT1-dependent cleavage of CYLD promotes NF-κB signaling and growth of aggressive B-cell receptor-dependent lymphomas
Authors: Minderman Marthe , Lantermans Hildo C. , Grüneberg Leonie J. , Cillessen Saskia A. G. M. , Bende Richard J. , van Noesel Carel J. M. , Kersten Marie José , Pals Steven T. , Spaargaren Marcel 
Published: March 2023

AbstractThe paracaspase mucosa-associated lymphoid tissue 1 (MALT1) is a protease and scaffold protein essential in propagating B-cell receptor (BCR) signaling to NF-κB. The deubiquitinating enzyme cylindromatosis (CYLD) is a recently discovered MALT1 target that can negatively regulate NF-κB activation. Here, we show that low expression of CYLD is associated with inferior prognosis of diffuse large B-cell lymphoma (DLBCL) and mantle cell lymphoma (MCL) patients, and that chronic BCR signaling propagates MALT1-mediated cleavage and, consequently, inactivation and rapid proteasomal degradation of CYLD. Ectopic overexpression of WT CYLD or a MALT1-cleavage resistant mutant of CYLD reduced phosphorylation of IκBα, repressed transcription of canonical NF-κB target genes and impaired growth of BCR-dependent lymphoma cell lines. Furthermore, silencing of CYLD expression rendered BCR-dependent lymphoma cell lines less sensitive to inhibition of NF-κΒ signaling and cell proliferation by BCR pathway inhibitors, e.g., the BTK inhibitor ibrutinib, indicating that these effects are partially mediated by CYLD. Taken together, our findings identify an important role for MALT1-mediated CYLD cleavage in BCR signaling, NF-κB activation and cell proliferation, which provides novel insights into the underlying molecular mechanisms and clinical potential of inhibitors of MALT1 and ubiquitination enzymes as promising therapeutics for DLBCL, MCL and potentially other B-cell malignancies.
Determining protein structures in cellular lamella at pseudo-atomic resolution by GisSPA
Authors: Cheng Jing , Liu Tong , You Xin , Zhang Fa , Sui Sen-Fang , Wan Xiaohua , Zhang Xinzheng 
Published: March 2023

AbstractCryo-electron tomography is a major tool used to study the structure of protein complexes in situ. However, the throughput of tilt-series image data collection is still quite low. Here, we show that GisSPA, a GPU accelerated program, can translationally and rotationally localize the target protein complex in cellular lamellae, as prepared with a focused ion beam, using single cryo-electron microscopy images without tilt-series, and reconstruct the protein complex at near-atomic resolution. GisSPA allows high-throughput data collection without the acquisition of tilt-series images and reconstruction of the tomogram, which is essential for high-resolution reconstruction of asymmetric or low-symmetry protein complexes. We demonstrate the power of GisSPA with 3.4-Å and 3.9-Å resolutions of resolving phycobilisome and tetrameric photosystem II complex structures in cellular lamellae, respectively. In this work, we present GisSPA as a practical tool that facilitates high-resolution in situ protein structure determination.
Testicular macrophages are recruited during a narrow fetal time window and promote organ-specific developmental functions
Authors: Gu Xiaowei , Heinrich Anna , Li Shu-Yun , DeFalco Tony 
Published: March 2023

AbstractA growing body of evidence demonstrates that fetal-derived tissue-resident macrophages have developmental functions. It has been proposed that macrophages promote testicular functions, but which macrophage populations are involved is unclear. Previous studies showed that macrophages play critical roles in fetal testis morphogenesis and described two adult testicular macrophage populations, interstitial and peritubular. There has been debate regarding the hematopoietic origins of testicular macrophages and whether distinct macrophage populations promote specific testicular functions. Here our hematopoietic lineage-tracing studies in mice show that yolk-sac-derived macrophages comprise the earliest testicular macrophages, while fetal hematopoietic stem cells (HSCs) generate monocytes that colonize the gonad during a narrow time window in a Sertoli-cell-dependent manner and differentiate into adult testicular macrophages. Finally, we show that yolk-sac-derived versus HSC-derived macrophages have distinct functions during testis morphogenesis, while interstitial macrophages specifically promote adult Leydig cell steroidogenesis. Our findings provide insight into testicular macrophage origins and their tissue-specific roles.
Short-term changes in ultrasound tomography measures of breast density and treatment-associated endocrine symptoms after tamoxifen therapy
Authors: Ramin Cody , Pfeiffer Ruth M. , Fan Sharon , Mullooly Maeve , Falk Roni T. , Jones Kristine , Caporaso Neil E. , Bey-Knight Lisa , Sak Mark A. , Simon Michael S. , Gorski David H. , Ali Haythem , Littrup Peter , Duric Neb , Sherman Mark E. , Gierach Gretchen L. 
Published: March 2023

AbstractAlthough breast density decline with tamoxifen therapy is associated with greater therapeutic benefit, limited data suggest that endocrine symptoms may also be associated with improved breast cancer outcomes. However, it is unknown whether endocrine symptoms are associated with reductions in breast density after tamoxifen initiation. We evaluated treatment-associated endocrine symptoms and breast density change among 74 women prescribed tamoxifen in a 12-month longitudinal study. Treatment-associated endocrine symptoms and sound speed measures of breast density, assessed via novel whole breast ultrasound tomography (m/s), were ascertained before tamoxifen (T0) and at 1–3 (T1), 4–6 (T2), and 12 months (T3) after initiation. CYP2D6 status was genotyped, and tamoxifen metabolites were measured at T3. Using multivariable linear regression, we estimated mean change in breast density by treatment-associated endocrine symptoms adjusting for age, race, menopausal status, body mass index, and baseline density. Significant breast density declines were observed in women with treatment-associated endocrine symptoms (mean change (95% confidence interval) at T1:−0.26 m/s (−2.17,1.65); T2:−2.12 m/s (−4.02,−0.22); T3:−3.73 m/s (−5.82,−1.63); p-trend = 0.004), but not among women without symptoms (p-trend = 0.18) (p-interaction = 0.02). Similar declines were observed with increasing symptom frequency (p-trends for no symptoms = 0.91; low/moderate symptoms = 0.03; high symptoms = 0.004). Density declines remained among women with detectable tamoxifen metabolites or intermediate/efficient CYP2D6 metabolizer status. Emergent/worsening endocrine symptoms are associated with significant, early declines in breast density after tamoxifen initiation. Further studies are needed to assess whether these observations predict clinical outcomes. If confirmed, endocrine symptoms may be a proxy for tamoxifen response and useful for patients and providers to encourage adherence.
The Ocean Carbon and Acidification Data System
Authors: Jiang Li-Qing , Kozyr Alex , Relph John M. , Ronje Errol I. , Kamb Linus , Burger Eugene , Myer Jonathan , Nguyen Liem , Arzayus Krisa M. , Boyer Tim , Cross Scott , Garcia Hernan , Hogan Patrick , Larsen Kirsten , Parsons A. Rost 
Published: March 2023

AbstractThe Ocean Carbon and Acidification Data System (OCADS) is a data management system at the National Oceanic and Atmospheric Administration (NOAA) National Centers for Environmental Information (NCEI). It manages a wide range of ocean carbon and acidification data, including chemical, physical, and biological observations collected from research vessels, ships of opportunity, and uncrewed platforms, as well as laboratory experiment results, and model outputs. Additionally, OCADS serves as a repository for related Global Ocean Observing System (GOOS) biogeochemistry Essential Ocean Variables (EOVs), e.g., oxygen, nutrients, transient tracers, and stable isotopes. OCADS endeavors to be one of the world’s leading providers of ocean carbon and acidification data, information, products, and services. To provide the best data management services to the ocean carbon and acidification research community, OCADS prioritizes adopting a customer-centric approach and gathering knowledge and expertise from the research community to improve its data management practices. OCADS aims to make all ocean carbon and acidification data accessible via a single portal, and welcomes submissions from around the world: https://www.ncei.noaa.gov/products/ocean-carbon-acidification-data-system/.
Newly reconstructed Arctic surface air temperatures for 1979–2021 with deep learning method
Authors: Ma Ziqi , Huang Jianbin , Zhang Xiangdong , Luo Yong , Ding Minghu , Wen Jun , Jin Weixin , Qiao Chen , Yin Yifu 
Published: March 2023

AbstractA precise Arctic surface air temperature (SAT) dataset, that is regularly updated, has more complete spatial and temporal coverage, and is based on instrumental observations, is critically important for timely monitoring and improving understanding of the rapid change in the Arctic climate. In this study, a new monthly gridded Arctic SAT dataset dated back to 1979 was reconstructed with a deep learning method by combining surface air temperatures from multiple data sources. The source data include the observations from land station of GHCN (Global Historical Climatology Network), ICOADS (International Comprehensive Ocean-Atmosphere Data Set) over the oceans, drifting ice station of Russian NP (North Pole), and buoys of IABP (International Arctic Buoy Programme). The last two are crucial for improving the representation of thein-situobserved temperatures within the Arctic. The newly reconstructed dataset includes monthly Arctic SAT beginning in 1979 and daily Arctic SAT beginning in 2011. This dataset would represent a new improvement in developing observational temperature datasets and can be used for a variety of applications.
Comprehensive characterization of B7 family members in NSCLC and identification of its regulatory network
Authors: Xiao Mintao , Pang Chunrong , Xiang Shixin , Zhao Yueshui , Wu Xu , Li Mingxing , Du Fukuan , Chen Yu , Wang Fang , Wen Qinglian , Xiao Zhangang , Yang Zhongming , Shen Jing 
Published: March 2023

AbstractB7 family members act as co-stimulatory or co-inhibitory molecules in the adaptive immune system. Thisstudy aimed to investigate the dysregulation, prognostic value and regulatory network of B7 family members in non-small cell lung cancer (NSCLC). Data for lung adenocarcinoma (LUAD) and lung squamous cell carcinoma (LUSC) patients were extracted from public databases. Patient prognosis was determined by Kaplan–Meier analysis. The downstream signaling pathways of B7 family were identified via GO and KEGG analysis. The key B7 related genes were selected by network, correlation and functional annotation analysis. Most B7 family members were dysregulated in LUAD and LUSC. The expression of B7-1/2/H3 and B7-H5 were significantly associated with overall survival in LUAD and LUSC, respectively. The major pathway affected by B7 family was the EGFR tyrosine kinase inhibitor resistance and ErbB signaling pathway. MAPK1, MAPK3 and MAP2K1 were pivotal B7 related genes in both LUAD and LUSC. This study reveals an overall dysregulation of B7 family members in NSCLC and highlights the potential of combination use of tyrosine kinase inhibitors or MEK/ERK inhibitors with B7 member blockade for NSCLC treatment.
Access to inclusive sanitation and participation in sanitation programs for people with disabilities in Indonesia
Authors: Daniel D. , Nastiti Anindrya , Surbakti Hana Yesica , Dwipayanti Ni Made Utami 
Published: March 2023

AbstractAccess to inclusive sanitation for people with disabilities (PWDs) remains a global challenge, including in Indonesia, where 10–15% of its population is disabled. Inclusive sanitation facilities can be achieved when PWDs are involved in the sanitation-related decision-making process, e.g., designing toilet that meets their needs. This study aims to investigate the situation of the sanitation facility in houses of PWDs and understand knowledge, attitude, and practices related to PWD participation in sanitation programs using a case study in two provinces in Indonesia: Nusa Tenggara Barat and Nusa Tenggara Timur. Quantitative data were taken from 129 PWD households, and qualitative data were from in-depth interviews with relevant stakeholders. The latter was presented in a causal loop diagram. Less than 5% had inclusive sanitation at home, mainly due to no supportive tools and bins. The knowledge levels related to sanitation and PWDs participation in sanitation programs were relatively low. Economic condition was one of the barriers for PWDs to participate in the sanitation program. Statistical analysis found that a higher household head education level was associated with a more positive attitude and higher PWD participation in sanitation programs. Although Indonesian law supports PWD participation, the implementation remains a challenge. This study also underlines the need for capacity building in facilitating PWDs’ involvement in sanitation programs and community meetings. Finally, barriers to PWD participation can come from different levels and actors, e.g., the family, the community, the district level, and the PWD itself, indicating the need to involve actors at all levels to enhance PWD participation in the sanitation program that leads to inclusive sanitation facilities for all groups.
Visioning future transport systems with an integrated robust and generative framework
Authors: Jittrapirom Peraphan , Bekius Femke , Führer Karoline 
Published: March 2023

AbstractVisioning has been widely adopted in transport planning as a method to support explorations of possible future transport systems over a long time horizon. There are vast variations in how visioning is applied but given a clear association between visions and the long-time perspective, it is unclear how these processes handle uncertainty surrounding the resulting visions and their implementation. This study reflects on previous visioning processes by systematically reviewing the scientific publications on participatory visioning in passenger transport. The review identifies possible improvements contributing to a systematic approach that produces concrete visions and actions to deal with uncertainties surrounding the vision and its implementation. We address these improvements by proposing a robust and generative visioning framework, which combines the generative approach in Appreciative Inquiry (Ai) and methods to handle uncertainty in the Dynamic Adaptive Planning (DAP). The framework is illustrated in a case study of the Southwest area of the Dutch city of the Hague that involved over 50 participants in a survey and two workshops. The process produced a vision for the mobility system of the area, a set of measures to realize it (i.e. pathways), and concrete actions to ensure that the pathways are robust against different futures that can affect the implementation. The approach can help planners, policymakers, and researchers in designing a visioning process that helps participants to better appreciate the temporal dimension of the visioning process and improves their awareness regarding the need to safeguard policy interventions against possible impacts of (un)certain future events.
Characterization and utility of two monoclonal antibodies to cholera toxin B subunit
Authors: Verjan Garcia Noel , Santisteban Celis Ian Carlosalberto , Dent Matthew , Matoba Nobuyuki 
Published: March 2023

AbstractCholera toxin B subunit (CTB) is a potent immunomodulator exploitable in mucosal vaccine and immunotherapeutic development. To aid in the characterization of pleiotropic biological functions of CTB and its variants, we generated a panel of anti-CTB monoclonal antibodies (mAbs). By ELISA and surface plasmon resonance, two mAbs, 7A12B3 and 9F9C7, were analyzed for their binding affinities to cholera holotoxin (CTX), CTB, and EPICERTIN: a recombinant CTB variant possessing mucosal healing activity. Both 7A12B3 and 9F9C7 bound efficiently to CTX, CTB, and EPICERTIN with equilibrium dissociation constants at low to sub-nanomolar concentrations but bound weakly, if at all, to Escherichia coli heat-labile enterotoxin B subunit. In a cyclic adenosine monophosphate assay using Caco2 human colon epithelial cells, the 7A12B3 mAb was found to be a potent inhibitor of CTX, whereas 9F9C7 had relatively weak inhibitory activity. Meanwhile, the 9F9C7 mAb effectively detected CTB and EPICERTIN bound to the surface of Caco2 cells and mouse spleen leukocytes by flow cytometry. Using 9F9C7 in immunohistochemistry, we confirmed the preferential localization of EPICERTIN in colon crypts following oral administration of the protein in mice. Collectively, these mAbs provide valuable tools to investigate the biological functions and preclinical development of CTB variants.
Vascular comorbidity is associated with decreased cognitive functioning in inflammatory bowel disease
Authors: Patel Ronak , Marrie Ruth Ann , Bernstein Charles N. , Bolton James M. , Graff Lesley A. , Marriott James J. , Figley Chase R. , Kornelsen Jennifer , Mazerolle Erin L. , Uddin Md Nasir , Fisk John D. ,   Comorbidity and Cognition in Multiple Sclerosis (CCOMS) Study Group, Bolton James , Graff Lesley , Kornelsen Jennifer , Mazerolle Erin , Patel Ronak , Figley Teresa D. , Helmick Carl A. 
Published: March 2023

AbstractReports of cognitive impairment in inflammatory bowel disease (IBD) have been mixed. IBD and cardiovascular disease are often co-morbid, yet it remains unknown whether vascular comorbidity confers a risk for decreased cognitive functioning, as observed in other populations. Participants with IBD were recruited from a longitudinal study of immune-mediated disease. Participants were administered a standardized neuropsychological test protocol, evaluating information processing speed, verbal learning and memory, visual learning and memory, and verbal fluency/executive function. Cognitive test scores were standardized using local regression-based norms, adjusting for age, sex, and education. Vascular risk was calculated using a modified Framingham Risk Score (FRS). We tested the association between FRS and cognitive test scores using a quantile regression model, adjusting for IBD type. Of 84 IBD participants, 54 had ulcerative colitis and 30 had Crohn’s disease; mean (SD) age was 53.36 (13.95) years, and a high proportion were females (n = 58). As the risk score (FRS) increased, participants demonstrated lower performance in information processing speed (β = − 0.12; 95% CI − 0.24, − 0.006) and verbal learning (β = − 0.14; 95% CI − 0.28, − 0.01) at the 50th percentile. After adjusting for IBD type and disease activity, higher FRS remained associated with lower information processing speed (β = − 0.14; 95% CI − 0.27, − 0.065). Vascular comorbidity is associated with lower cognitive functioning in persons with IBD, particularly in the area of information processing speed. These findings suggest that prevention, identification, and treatment of vascular comorbidity in IBD may play a critical role for improving functional outcomes in IBD.