bluuebunny/crossref_metadata_2025_split huggingface dataset:
Prospective assessment of cytokines and regulatory proteins concentration in the tear fluid of POAG patients with various hypotensive effects after non-penetrating deep sclerectomy
Authors: Malisheva J. V. , Iureva T. N. , Volkova N. V. , Kursakova J. V. , Kolesnikov S. I. 
Published: May 2023

The aim. To assess the dynamics of cytokine content in tear fluid of primary openangle glaucoma (POAG) patients at various terms after non-penetrating deep sclerectomy (NPDS) in relation to the functional state of the outflow tracts.Material and methods. We carried out prospective examination of 65 patients with advanced stage of primary open-angle glaucoma after NPDS. Depending on the course of the postoperative period and the conditions for achieving the hypotensive effect of NPDS, all patients were divided into three groups: group 1 – with the optimal hypotensive effect; group 2 – with the conditional hypotensive effect; group 3 – with no hypotensive effect after NPDS. The intraocular pressure and the concentration of TGF-β, MMP-9, IL-6, IL-8, VEGF-A (121 and 165) in the tear fluid were studied using ELISA method in pre- and postoperative period. We studied the outflow tracts using optical coherence tomography and carried out ultrastructural analysis of filtering blebs tissue.Results. In group 1, the minimum initial concentrations of IL-6, IL-8 and TGF-β in the tear fluid and their moderate increase in the postoperative period; high concentrations of MMP-9 at all stages and an increase in VEGF-A by 2 months after NPDS were registered. In group 2, there was a high level of VEGF-A in the tear fluid before and 2 months after NPDS, an increase in TGF-β, IL-6 and IL-8 in the tear fluid in the early period with their suppression in the late period, as well as an increase in MMP-9 in the early postoperative period. Group 3 had maximum concentrations of TGF-β and IL-8 in the tear fluid initially and in the early postoperative period, suppression of MMP-9 in the tear fluid 2 weeks after and of VEGF-A 2 months after NPDS.Conclusion. Initially high concentrations of IL-6, IL-8, and TGF-β in the tear fluid and the suppression of MMP-9 and VEGF-A in the postoperative period contribute to the surgical failure of the NPDS.
Component composition of the body in children with chronic kidney disease according to the results of bioimpedansometry
Authors: Zavyalova A. N. , Lebedev D. A. , Novikova V. P. , Smirnova N. N. , Firsova L. A. 
Published: May 2023

Body composition reflects the dynamic processes in a child’s development. The recommended restrictive diets for patients with advanced chronic kidney disease (CKD) contribute to a high risk of sarcopenic muscle wasting as diagnosed by bioimpedancemetry.The aim of the study. To assess BMI and body composition in children with CKD, to identify features of body composition in patients with different BMI Z-score values.Materials and methods. The physical development of 110 children with CKD of different stages was assessed. Patients were divided into two clusters: Group 1 (92 children) with BMI from 10.95 to 21.5 kg/m2, BMI Z-score did not exceed +2.0 (without obesity); Group 2 (18 children) – BMI from 24.11 to 37.2 kg/m2, Z-score BMI – more than +2.0 (obese). All underwent bioimpedancemetry, the proportion of fat and active cell mass was assessed. The comparison was carried out by nonparametric statistics methods.Results. Changes in body composition were revealed: children without obesity had severe protein-energy deficiency in 7 cases (7.6 %). The difference in the proportion of fat mass in children of different groups, Me [Q1; Q3]: Group 1 – 18.00 % [14.00; 22.00], Group 2 – 35.00 % [21.98; 41.00], (Mann – Whitney U-test: U = 279.5, p = 0.00001). In Group 1, the active cell mass was 53.50 % [51.00; 56.00], in Group 2 – 41.50 % [39.00; 47.00] (U = 174.5, p = 0.000001), there were no significant differences in other parameters of bioimpedancemetry.Conclusions. The proportion of active cell mass is lower in overweight children, with a significant predominance of the proportion of fat mass, which indicates the depletion of protein reserves due to their redistribution and possible insufficient alimentary intake in advanced stages of CKD.
High sleep reactivity: clinical, psychological and polysomnographic features
Authors: Zabroda E. N. , Gordeev A. D. , Amelina V. V. , Bochkarev M. V. , Osipenko S. I. , Korostovtseva L. S. , Sviryaev Yu. V. 
Published: May 2023

Background. The model of sleep reactivity to stress considers sleep reactivity to stress as a link in the pathogenesis of insomnia disorder – the degree to which stress disturbs sleep, which manifests as difficulty in initiating and maintaining sleep.The aim. To study clinical and psychological features as well as subjective and objective sleep indexes of subjects with high level of sleep reactivity to stress.Materials and methods. The psychological status, subjective indexes of sleep and sleep reactivity to stress according to Ford Insomnia Response to Stress Test were studied among 18–75 year-old subjects without significant sleep disturbances and patients with chronic insomnia. Polysomnography was performed for objective evaluation of sleep parameters.Results. It was found that individuals with high levels of sleep reactivity to stress were characterized by high levels of anxiety, restlessness, and neuroticism. According to results of Pittsburg questionnaire, a lower quality of sleep was revealed. These findings were correlated with objective indexes of sleep according to polysomnographic studies: less deep sleep and its lower efficiency due to sleep disturbances.Conclusions. Individuals with high sleep reactivity to stress are characterized by greater anxiety combined with subjective and objective sleep disturbance like insomnia type.
Assessment of the effectiveness of restrictive epidemic control measures using original models of cellular automaton
Authors: Karateev A. Yu. 
Published: May 2023

Background. The ongoing COVID-19 pandemic, the human casualties caused by it, and the possibility of new epidemical threats make the search for effective countermeasures actual. One of the most effective tools, as the experience of the COVID-19 pandemic has shown, is restrictive measures of various types, which are especially significant with medical countermeasures being unavailable or insufficient. At the same time, the topic of restrictive measures and their mathematical modeling, especially given its importance, is not sufficiently disclosed in the scientific literature.The aim. To determine the possibility of assessing the effectiveness of restrictive epidemic control measures using original models of cellular automaton with intercellular boundaries.Methods. To determine the impact of restrictive measures on the dynamics of the daily increase in infected people, an original cellular automaton with intercellular boundaries was developed, which makes it possible to simulate epidemic control measures of varying stringency. In the simulations carried out using the Monte Carlo method with subsequent statistical processing, we studied the impact of restrictive measures of varying stringency on the number of infected people, the duration of the epidemic, and the quality of forecasting. The final series of experiments simulated the spread of the COVID-19 virus in Germany in the first half of 2020.The results show that even a simple cellular automaton model with boundaries successfully describes the course of the epidemic and allows us to assess the effectiveness of restrictive measures. The dependence of the daily increase in infected people on the stringency of measures is presented; it is shown what characteristics of the population can influence this dependence. It was found that the measures of medium stringency (40–50 % according to the Stringency Index) have the least predictable effect; they can cause both rapid localization of the focus and the spread of the epidemic to a large part of the population. Weak and strong measures give a more predictable effect.Conclusion. Cellular automaton models with intercellular boundaries have great potential for modeling the impact of restrictive measures on the course of an epidemic, making it possible to predict the dynamics of infected people based on the population data and the restrictive measures being introduced.
The first TIPS surgery performed in the Udmurt Republic in a young patient with secondary biliary cirrhosis
Authors: Styazhkina S. N. , Zaitsev D. V. , Bagautdinov A. L. , Sharafutdinov M. R. , Antropova Z. A. , Zaripov I. I. , Kamalov M. I. 
Published: May 2023

Treatment of patients with iatrogenic injuries of the biliary tract is the most difficult and important section in hepatobiliary surgery. When analyzing the causes, it was found that in 70–94 % of cases this type of injury is observed during cholecystectomy. We present a rare clinical case of the development of secondary biliary cirrhosis due to iatrogenic trauma of the biliary tract. This injury caused long-term suffering for the patient due to the further development of complications of cirrhosis, specifically of portal hypertension. The latter caused repeated recurrent profuse bleeding from varicose veins of the esophagus. The use of a minimally invasive transjugular intrahepatic portosystemic shunt procedure for the first time in the Udmurt Republic was of particular relevance in solving this problem. This procedure has become a key one in solving the abovementioned problems and will become the preventive measure for the cirrhosis progression in the future. It is also important to focus on prophylactic measures aimed at preventing iatrogenic injuries of the biliary tract, as this problem can cause irreversible complications. Prevention should include adequate examination and visualization of the bile ducts and gallbladder before surgery, their careful mobilization, compliance with the rules of operation with electrosurgical instruments, as well as the use of additional minimally invasive techniques such as choledoscopy, cholangiography and intraoperative ultrasound. Besides that, all manipulations should be carried out under strict control and clear visualization of instruments and anatomical structures of organs. The article provides a detailed description of the technique of transjugular intrahepatic portosystemic shunt surgery, as well as presents X-ray images obtained during this operation.
Features of the integration of two-layer metal knitwear made of titanium nickelide during the replacement of a thoracoabdominal defect in the experiment
Authors: Topolnitskiy E. B. , Shefer N. A. , Marchenko E. S. , Fomina T. I. , Mikhed R. A. , Tsydenova А. N. , Garin A. S. 
Published: May 2023

The aim of investigation was to study experimentally the morphological features of tissue integration of two-layer titanium nickelide (TiNi) knitwear when replacing thoracoabdominal defects.Materials and methods. The experiments were carried out on 40 Wistar rats. The experimental animals were divided into two comparison groups: in Group A (n = 20) the defect was replaced using a two-layer knitted tape made of TiNi, in Group B (n = 20) a polypropylene mesh implant was used. The technique of the operation and the peculiarities of keeping the animals did not differ. Animals were taken out after 14, 30, 60 and 90 days of experiment. The macroscopic structural features at the site of implant fixation to tissues and at the sites of contact with underlying organs were studied, and the inflammatory process was assessed. The histological and electron microscopic study was carried out with an assessment of the features of tissue integration through the mesh structure of knitwear.Results. Thirty days after the surgery in four cases of Group B the appearance of the chest wall deformation at the site of implant fixation was noted, in one case the deformation site was located along the lateral edge of the abdominal wall. Among the animals of Group A no such changes were recorded. The histological and electron microscopy examination revealed that the porous structure of the TiNi wire, as well as the biomechanical and biochemical properties of the two-layer metal knitwear, ensure optimal integration of the endoprosthesis in the body tissues, forming an elastic frame close to natural. In Group B, on the contrary, the reaction of the body caused by the implanted polypropylene prosthesis was characterized by more pronounced fibrosis, and tissue integration through the mesh structure of the implant was not observed.Conclusion. Two-layer TiNi knitwear in the replacement of complex structures of the thoracoabdominal zone showed promising results, which opens up prospects for further clinical research.
Epidemiological manifestations of tuberculosis infection in the Omsk region: dynamics and trends
Authors: Kostyukova I. V. , Pasechnik O. A. , Mokrousov I. V. 
Published: May 2023

Background. Tuberculosis (TB) infection remains relevant as one of the leading public health problems in Russia.The aim. To characterize the dynamics and trends of epidemiological manifestations of TB infection in the Omsk region.Materials and methods. An observational descriptive-evaluative epidemiological study was carried out in the Omsk region from 2009 to 2021. The data available in the Federal statistical observation forms NN 7, 8, and 33, and results of the bacteriological study of patients with respiratory TB were analyzed.Results. In the Omsk region, an improvement in the epidemiological situation was observed from 2009 to 2021. It was associated with a decrease in TB prevalence by 3.7 times (from 325.6 to 86.7), mortality – by 5.8 times (from 21.1 to 3.6), incidence – by 2.7 times (from 130.7 to 48.4 per 100,000). At the same time, proportion of microscopy/culture-positive patients infected with multidrug-resistant Mycobacterium tuberculosis strains increased from 9.2 to 29.8 % among all patients, and from 15.5 to 30.6 % among newly diagnosed patients. There was a trend towards an increase in the number of cases with primary extensive drug resistance of M. tuberculosis. The incidence of tuberculosis associated with HIV infection has increased 10 times and reached 15.6 per 100,000 population.Conclusions. In the Omsk region, there is a change in the structure of M. tuberculosis strains with a predominance of multiple and extensive drug resistance along with decrease in TB incidence and mortality. New approaches are needed to organize the system of epidemiological surveillance and control of TB infection.
The role of circulating miR-19b miRNA in predicting the outcome of COVID-19
Authors: Shkurnikov M. Yu. , Kolesnikov S. I. 
Published: May 2023

Background. MicroRNAs are short (20–22 nucleotides) non-coding RNAs that can posttranscriptionally regulate gene expression and are considered a regulator of the innate immunity system. Previously, many papers were published on the prediction of the interaction of the single-stranded (+)RNA virus SARS-CoV-2 with human microRNAs, as well as on the profile of circulating microRNAs in patients with COVID-19 of varying severity. However, no works are analyzing the possible contribution of miRNAs circulating in blood plasma to the severity of COVID-19.The aim. To study the features of the blood plasma microRNA profile of patients with different severity of the new coronavirus infection COVID-19 and to evaluate the possibility of microRNA interaction with the SARS-CoV-2 genome.Materials and methods. The results of NGS sequencing of plasma miRNAs of 3 recovered and 8 deceased patients with a highly severe form of COVID-19 were studied. Differentially presented microRNAs were determined using bioinformatics methods, and their binding sites with the SARS-CoV-2 genome were predicted.Results. This study demonstrates that in patients who have recovered from a highly severe form of COVID-19, the level of hsa-miR-19b-3p in the blood plasma is significantly increased. This microRNA makes up about 1.5 % of all circulating microRNAs and can bind to SARS-CoV-2 regions encoding proteins that suppress intracellular immunity mechanisms (NSP3, NSP9). In addition, this miRNA can stimulate the functional activity and proliferation of cytotoxic T-lymphocytes, one of the critical components of acquired cellular immunity against SARS-CoV-2.Conclusion. The results of the study can be used in the development of antiviral drugs based on RNA interference, as well as in the development of predictive test systems to optimize the tactics of treating patients with COVID-19.
Thyroid status and TNF-alpha in post-reproductive women with COVID-19 and 12 months after the disease
Authors: Semenova N. V. , Kolesnikov S. I. , Vyrupaeva E. V. , Sholokhov L. F. , Rychkova L. V. , Petrova А. G. , Akhmedzyanova M. R. , Darenskaya M. A. , Kolesnikova L. I. 
Published: May 2023

The aim. To assess the thyroid status and its relationship with tumor necrosis factor alpha (TNF-alpha) in post-reproductive women in the acute phase of the COVID-19 of moderate course and 12 months after the disease.Materials and methods. 85 women aged 45 to 69 years were divided into groups: women without COVID-19, not vaccinated, with no antibodies to COVID-19 (IgG) – control group (n = 15); women in the acute phase of COVID-19 of a moderate course, accompanied by pneumonia – main group (n = 57); patients from the main group who agreed to be examined 12 months after having COVID-19 (n = 14); women with IgG in blood who deny any symptoms of COVID-19 in the last 12 months – asymptomatic COVID-19 (n = 13). Using hormone replacement therapy and the presence of thyroid disease in history were the exclusion criteria from the study.Results. 75.4 % of patients with COVID-19 had euthyroidism, 12.3 % had subclinical hyperthyroidism. An increase of free thyroxine (free T4) level in women with COVID-19 as compared to the control group (p = 0.004) and the group with asymptomatic COVID-19 (p = 0.054) was found. There was no statistically significant difference in the level of thyroid stimulating hormone between the groups. The level of C-reactive protein in women with COVID-19 was naturally higher as compared to the control group (p = 0.009) and the group of asymptomatic patients (p = 0.001). A lower TNF-alpha level was found in the group of patients without clinical signs of COVID-19 as compared to the control group (p = 0.007) and the group with COVID-19 (p = 0.00007). The analysis of correlation relationships revealed a positive correlation between of free T4 and TNF-alpha levels in women with COVID-19 (r = 0.38, p = 0.004).Conclusions. The moderate course of COVID-19 in the post-reproductive women is associated with an increase of free T4 level, which positively correlates with TNF-alpha level. Twelve months after COVID-19, thyroid status in women remains at the level of the acute phase of the disease.
The enhanced recovery program in urology. Systematic review and meta-analysis
Authors: Vorobev V. A. , Beloborodov V. A. , Tukhiev A. R. 
Published: May 2023

A systematic review and meta-analysis of data on the problem of enhanced recovery in urology was performed. Inclusion criteria – clinical trials of the enhanced recovery program in urology.The aim of the study was to assess the significance of the enhanced recovery program (ERP) in the provision of surgical care in the “urology” profile.Materials and methods. The systematic review was performed according to the guidelines for the presentation of systematic reviews and meta-analyses by PRISMA. The registration number in the international system Prospero was received (CRD42022358982). The review included 364 studies. Studies in urologic oncology were excluded from the meta-analysis. The meta-analysis included 15 studies involving 2293 subjects. A comparison was made between the application of ERP and the standard treatment protocol.Results. The use of ERP leads to an expected two-fold reduction in the duration of postoperative length of hospitalization (OR = –1.96; 95% CI: –2.56÷–1.36; p < 0.00001). The reduction in the duration of hospitalization with the use of ERP in urology does not lead to the increased risk of readmission or re-operation (p = 0.35). The risks of developing postoperative complications ≥ Class 2 by Clavien – Dindo classification were comparable in both groups (p = 0.13). The use of ERP increases the expected success of the treatment by 1.74 times (OR = 1.74; 95% CI: 1.08–2.79; p = 0.02). With the use of ERP in reconstructive urology, a significantly lower risk of complications was established (p = 0.02).Conclusion. The ERP program allows you to reduce the time and cost of treatment, reduce the likelihood of re-hospitalization and achieve better treatment results. The use of ERP is not accompanied by increased risk of complications > Class 2.
Methods of physical rehabilitation of elderly people for the prevention and treatment of sarcopenia
Authors: Pleshchev I. E. , Achkasov E. E. , Nikolenko V. N. , Shkrebko A. N. , Ivanova I. V. 
Published: May 2023

The aim of the review is to analyze the prevalence of sarcopenia in the elderly age group, the causes of its occurrence, and to present modern methods of prevention and physical rehabilitation. The study focuses on the relationship between exercise, training effects and physiological mechanisms, as well as the safety of various types of strength, anaerobic and multimodal training, which have a positive impact during the prevention and rehabilitation treatment of sarcopenia. Literature reviews, meta-analyses, and original studies are included that focus on older people in all settings, using validated assessment tools and methods. A literature search was conducted in four electronic databases – PubMed, Cochrane Library, Scopus, Springer, for the period from 2012 to June 30, 2022. There were no restrictions on the language bias of the publication. Search strategy. The keywords used to define the terms of participation in the review are “older/advanced age”, “sarcopenia” and “sarcopenic obesity”. Articles were included if they met the following criteria – cohorts with mean or median age ≥ 60 years and any of the following definitions of sarcopenia: European Working Group on Sarcopenia in the Elderly (EWGSOP), Asian Working Group on Sarcopenia (AWGS), International Working Group on Sarcopenia (IWGS). To ensure comparability of interventions, the review included studies that were conducted for at least 8 weeks, and the distribution of patients by study design was randomized. Also, articles involving hospitalized patients are excluded.