bluuebunny/crossref_metadata_2025_split huggingface dataset:
Research on psychological attributions and intervention strategies of new energy hybrid vehicle purchase behavior
Authors: Guo Qing , You Wenlan 
Published: June 2023

AbstractBased on the questionnaire survey, this paper developed a theoretical model of the factors influencing consumers' purchase behavior for new energy hybrid vehicles using the theory of planned behavior and structural equation modeling techniques. It then used SPSS and AMOS to perform factor analysis, model fitness, and path analysis to reach the following conclusions: perceived behavioral control, behavioral attitude, and subjective norm have a significant positive influence on behavioral intention, and behavioral intention has a significant influence on actual behavior. However, there is no significant effect of perceived behavioral control on actual purchase behavior, but rather an indirect effect on actual behavior through the mediating variable of behavioral intention. The analysis of the multi-group model based on the individual characteristics of consumers showed that the coefficient of subjective norm on behavioral intention was higher for extroverted consumers than for introverted consumers; the influence of behavioral attitude on behavioral intention was significantly higher for introverted consumers than for subjective norm on behavioral intention.
Stacked ensemble machine learning for porosity and absolute permeability prediction of carbonate rock plugs
Authors: Kalule Ramanzani , Abderrahmane Hamid Ait , Alameri Waleed , Sassi Mohamed 
Published: June 2023

AbstractThis study employs a stacked ensemble machine learning approach to predict carbonate rocks' porosity and absolute permeability with various pore-throat distributions and heterogeneity. Our dataset consists of 2D slices from 3D micro-CT images of four carbonate core samples. The stacking ensemble learning approach integrates predictions from several machine learning-based models into a single meta-learner model to accelerate the prediction and improve the model's generalizability. We used the randomized search algorithm to attain optimal hyperparameters for each model by scanning over a vast hyperparameter space. To extract features from the 2D image slices, we applied the watershed-scikit-image technique. We showed that the stacked model algorithm effectively predicts the rock's porosity and absolute permeability.
Evolving relationship of Nares Strait ice arches on sea ice along the Strait and the North Water, the Arctic’s most productive polynya
Authors: Moore G. W. K. , Howell S. E. L. , Brady M. 
Published: June 2023

AbstractNares Strait, the waterway that separates northwest Greenland from Ellesmere Island, is a major pathway along which sea ice leaves the Arctic, including the planet’s oldest and thickest sea ice that is experiencing an accelerated loss. Ice arches that develop during the winter at the Strait’s northern or southern terminus can remain stable for months at a time during which the transport of sea ice ceases. The Arctic’s most productive polynya, the North Water (NOW) orPikialasorsuaq(West Greenlandic for ‘great upwelling’) forms at the Strait’s southern end. There is evidence that a warming climate and the concomitant thinning of Arctic sea ice is weakening the arches and it has been proposed that this may impact the stability of NOW and the complex ecosystem that it sustains. Here we employ a categorization of recent winters with respect to the presence or absence of ice arches to explore their impact on sea ice along the Strait and over the NOW. We find that winters during which a southern ice arch is absent are associated with a reduced and thinner ice cover along the Strait with ice conditions over the NOW similar to that during winters with a southern arch. In winters, without a southern arch, there is also an acceleration of the winds along the Strait that contributes to the presence of reduced ice cover. Ocean color remote sensing data suggests that current levels of primary productivity over the NOW are independent of the presence or absence of an ice arch. The results suggest more research is needed to assess the stability of the NOW, with respect to reduced ice cover and primary productivity, in a future where ice arches cease to form along Nares Strait.
Interplay between uncertainty intolerance, emotion regulation, cognitive flexibility, and psychopathology during the COVID-19 pandemic: a multi-wave study
Authors: Godara Malvika , Everaert Jonas , Sanchez-Lopez Alvaro , Joormann Jutta , De Raedt Rudi 
Published: June 2023

AbstractThe COVID-19 pandemic has created a significant mental health burden on the global population. Studies during the pandemic have shown that risk factors such as intolerance of uncertainty and maladaptive emotion regulation are associated with increased psychopathology. Meanwhile, protective factors such as cognitive control and cognitive flexibility have been shown to protect mental health during the pandemic. However, the potential pathways through which these risk and protective factors function to impact mental health during the pandemic remain unclear. In the present multi-wave study, 304 individuals (18 years or older, 191 Males), residing in the USA during data collection, completed weekly online assessments of validated questionnaires across a period of five weeks (27th March 2020–1st May 2020). Mediation analyses revealed that longitudinal changes in emotion regulation difficulties mediated the effect of increases in intolerance of uncertainty on increases in stress, depression, and anxiety during the COVID-19 pandemic. Further, individual differences in cognitive control and flexibility moderated the relationship between intolerance of uncertainty and emotion regulation difficulties. While intolerance of uncertainty and emotion regulation difficulties emerged as risk factors for mental health, cognitive control and flexibility seems to protect against the negative effects of the pandemic and promote stress resilience. Interventions aimed at enhancing cognitive control and flexibility might promote the protection of mental health in similar global crises in the future.
Screening macroalgae for mitigation of enteric methane in vitro
Authors: Wasson D. E. , Stefenoni H. , Cueva S. F. , Lage C. , Räisänen S. E. , Melgar A. , Fetter M. , Hennessy M. , Narayan K. , Indugu N. , Pitta D. , Yarish C. , Hristov A. N. 
Published: June 2023

AbstractThis study investigated the effects of 67 species of macroalgae on methanogenesis and rumen fermentation in vitro. Specimens were analyzed for their effect on ruminal fermentation and microbial community profiles. Incubations were carried out in an automated gas production system for 24-h and macroalgae were tested at 2% (feed dry matter basis) inclusion rate. Methane yield was decreased 99% byAsparagopsis taxiformis(AT) when compared with the control.Colpomenia peregrinaalso decreased methane yield 14% compared with control; no other species influenced methane yield. Total gas production was decreased 14 and 10% by AT andSargassum hornericompared with control, respectively. Total volatile fatty acid (VFA) concentration was decreased between 5 and 8% by 3 macroalgae, whereas AT reduced it by 10%. Molar proportion of acetate was decreased 9% by AT, along with an increase in propionate by 14%.Asparagopsis taxiformisalso increased butyrate and valerate molar proportions by 7 and 24%, respectively, whereas 3 macroalgae species decreased molar proportion of butyrate 3 to 5%.Vertebrata lanosaincreased ammonia concentration, whereas 3 other species decreased it. Inclusion of AT decreased relative abundance ofPrevotella, Bacteroidales, Firmicutes and Methanobacteriaceae,whereasClostridium, AnaerovibrioandMethanobrevibacterwere increased. Specific gene activities forMethanosphaera stadtmaneandMethanobrevibacter ruminantiumwere decreased by AT inclusion. In this in vitro study,Asparagopsis taxiformiswas most effective in decreasing methane concentration and yield, but also decreased total gas production and VFA concentration which indicates overall inhibition of ruminal fermentation. No other macroalgae were identified as potential mitigants of enteric methane.
miRNAs signature as potential biomarkers for cervical precancerous lesions in human papillomavirus positive women
Authors: González-Ramírez Martha I. , Cardona Yurley T. , Agudelo María C. , López Carolina , Florez-Acosta Juan J. , Agudelo-Gamboa Samuel , Garai Jone , Li Li , Orozco-Castaño Carlos A. , Zabaleta Jovanny , Sánchez Gloria I. 
Published: June 2023

AbstractBiomarkers to identify women at risk of cervical cancer among those with high-risk HPV infection (hrHPV+) are needed. Deregulated expression of microRNAs (miRNAs) contributes to hrHPV-induced cervical carcinogenesis. We aimed at identifying miRNAs with the capacity to distinguish high (CIN2+) and low (≤ CIN1) grade cervical lesions. We sequenced miRNA libraries from Formalin-Fixed Paraffin-Embedded (FFPE) tissues from women with CIN2+ (n = 10) and age-matched women with ≤ CIN1 (n = 10), randomly and retrospectively selected from a trial that followed women for 24 months after a hrHPV+ test at the screening visit. Five miRNAs differentially expressed were validated by RT-qPCR in an independent set of FFPE tissues with a reviewed diagnosis of CIN2+ (n = 105) and ≤ CIN1 (n = 105). The Ingenuity Pathway Analysis (IPA) was conducted to identify mRNAs inversely correlated with the top 25 differentially expressed miRNAs. Inverse correlations with 401 unique mRNA targets were identified for fourteen of the top 25 differentially expressed miRNAs. Eleven of these miRNAs targeted 26 proteins of pathways deregulated by HPV E6 and E7 oncoproteins and two of them, miR-143-5p and miR-29a-3p, predicted CIN2+ and CIN3+ in the independent validation by RT-qPCR of FFPE tissues from hrHPV-positive women.
Diagnostic performance of calf circumference, SARC-F, and SARC-CalF for possible sarcopenia screening in Indonesia
Authors: Kandinata Stefanus G. , Widajanti Novira , Ichwani Jusri , Firdausi Hadiq , Aryana I. G. P. S. , Alkaff Firas F. 
Published: June 2023

AbstractAsian working group for sarcopenia (AWGS) recently introduced “possible sarcopenia” diagnosis for early identification of sarcopenia in the primary healthcare. For initial screening, 3 modalities, i.e. calf circumference (CC) measurement, strength, assistance with walking, rising from a chair, climbing stairs, and falls (SARC-F) questionnaire, and a combination of both (SARC-CalF), are recommended. However, no validation study has been done until now. Therefore, this study aims to evaluate the diagnostic performance of the recommended screening modalities using data from Indonesia. This cross-sectional study included subjects aged ≥ 60 years old who visited primary healthcare in Surabaya, Indonesia. The diagnosis of possible sarcopenia was confirmed with hand-grip strength and repeated chair stand test. Receiver operating characteristic curve analysis was used to evaluate the diagnostic performance. Among 266 subjects, 186 (70%) were diagnosed with possible sarcopenia. Using the recommended cut-off, the area under the curve, sensitivity, and specificity were 0.511, 48.39% and 53.75% for CC, 0.543, 8.60% and 100% for SARC-F, and 0.572, 19.35% and 95% for SACRC-CalF. Our findings indicate that the diagnostic performance of the recommended screening modalities is poor. Multicenter studies from different areas in Indonesia should be done to confirm these findings.
Tozorakimab (MEDI3506): an anti-IL-33 antibody that inhibits IL-33 signalling via ST2 and RAGE/EGFR to reduce inflammation and epithelial dysfunction
Authors: England Elizabeth , Rees D. Gareth , Scott Ian Christopher , Carmen Sara , Chan Denice T. Y. , Chaillan Huntington Catherine E. , Houslay Kirsty F. , Erngren Teodor , Penney Mark , Majithiya Jayesh B. , Rapley Laura , Sims Dorothy A. , Hollins Claire , Hinchy Elizabeth C. , Strain Martin D. , Kemp Benjamin P. , Corkill Dominic J. , May Richard D. , Vousden Katherine A. , Butler Robin J. , Mustelin Tomas , Vaughan Tristan J. , Lowe David C. , Colley Caroline , Cohen E. Suzanne 
Published: June 2023

AbstractInterleukin (IL)-33 is a broad-acting alarmin cytokine that can drive inflammatory responses following tissue damage or infection and is a promising target for treatment of inflammatory disease. Here, we describe the identification of tozorakimab (MEDI3506), a potent, human anti-IL-33 monoclonal antibody, which can inhibit reduced IL-33 (IL-33red) and oxidized IL-33 (IL-33ox) activities through distinct serum-stimulated 2 (ST2) and receptor for advanced glycation end products/epidermal growth factor receptor (RAGE/EGFR complex) signalling pathways. We hypothesized that a therapeutic antibody would require an affinity higher than that of ST2 for IL-33, with an association rate greater than 107 M−1 s−1, to effectively neutralize IL-33 following rapid release from damaged tissue. An innovative antibody generation campaign identified tozorakimab, an antibody with a femtomolar affinity for IL-33red and a fast association rate (8.5 × 107 M−1 s−1), which was comparable to soluble ST2. Tozorakimab potently inhibited ST2-dependent inflammatory responses driven by IL-33 in primary human cells and in a murine model of lung epithelial injury. Additionally, tozorakimab prevented the oxidation of IL-33 and its activity via the RAGE/EGFR signalling pathway, thus increasing in vitro epithelial cell migration and repair. Tozorakimab is a novel therapeutic agent with a dual mechanism of action that blocks IL-33red and IL-33ox signalling, offering potential to reduce inflammation and epithelial dysfunction in human disease.
Association between interatrial septum adiposity and atrial fibrillation: transesophageal echocardiography imaging and autopsy study
Authors: Miyoshi Miho , Abe Ichitaro , Kodama Nozomi , Zhan Yinge , Kira Shintaro , Ishii Yumi , Harada Taisuke , Takano Masayuki , Takahashi Masaki , Sato Hiroki , Tawara Katsunori , Kondo Hidekazu , Fukui Akira , Fukuda Tomoko , Akioka Hidefumi , Shinohara Tetsuji , Teshima Yasushi , Yufu Kunio , Nakagawa Mikiko , Daa Tsutomu , Shimada Tatsuo , Takahashi Naohiko 
Published: June 2023

AbstractRecent clinical evidence has suggested that interatrial septal (IAS) adiposity contributes to atrial fibrillation (AF). The present study aimed to confirm the usefulness of transesophageal echocardiography (TEE) to estimate IAS adiposity in patients with AF. The histological IAS analysis based on autopsy samples sought to clarify characteristics that underlie the contribution of IAS adiposity to AF. The imaging study analyzed the TEE results in patients with AF (n = 184) in comparison with transthoracic echocardiography (TTE) and computed tomography (CT) results. The autopsy study histologically analyzed IAS in subjects with (n = 5) and without (n = 5) history of AF. In the imaging study, the ratio of interatrial septum adipose tissue (IAS-AT) volume per epicardial adipose tissue (EpAT) volume was greater in patients with persistent AF compared (PerAF) to those with paroxysmal AF (PAF). Multivariable analysis revealed that both TEE-assessed IAS thickness and TTE-assessed left atrial dimension were predicted by CT-assessed IAS-AT volume. In the autopsy study, the histologically-assessed IAS section thickness was greater in the AF group than that in the non-AF group and was positively correlated with the IAS-AT area percentage. In addition, the size of adipocytes in IAS-AT was smaller, compared to EpAT and subcutaneous adipose tissue (SAT). IAS-AT infiltrated into the IAS myocardium, as if adipose tissue split the myocardium (designated as myocardial splitting by IAS-AT). The number of island-like myocardium pieces as a result of myocardial splitting by IAS-AT was greater in the AF group than in the non-AF group and was positively correlated with the IAS-AT area percentage. The present imaging study confirmed the usefulness of TEE to estimate IAS adiposity in patients with AF without radiation exposure. The autopsy study suggested that the myocardial splitting by IAS-AT may contribute to atrial cardiomyopathy leading to AF.
Fatty acid fingerprints in bronchoalveolar lavage fluid and its extracellular vesicles reflect equine asthma severity
Authors: Höglund Nina , Nieminen Petteri , Mustonen Anne-Mari , Käkelä Reijo , Tollis Sylvain , Koho Ninna , Holopainen Minna , Ruhanen Hanna , Mykkänen Anna 
Published: June 2023

AbstractEquine asthma (EA) is an inflammatory disease of the lower airways driven by mediators released from cells. Extracellular vesicles (EVs) are vehicles for lipid mediators, which possess either pro-inflammatory or dual anti-inflammatory and pro-resolving functions. In this study, we investigated how the respiratory fatty acid (FA) profile reflects airway inflammatory status. The FA composition of bronchoalveolar lavage fluid (BALF), BALF supernatant, and bronchoalveolar EVs of healthy horses (n = 15) and horses with mild/moderate EA (n = 10) or severe EA (SEA, n = 5) was determined with gas chromatography and mass spectrometry. The FA profiles distinguished samples with different diagnoses in all sample types, yet they were insufficient to predict the health status of uncategorized samples. Different individual FAs were responsible for the discrimination of the diagnoses in different sample types. Particularly, in the EVs of SEA horses the proportions of palmitic acid (16:0) decreased and those of eicosapentaenoic acid (20:5n-3) increased, and all sample types of asthmatic horses had elevated dihomo-γ-linolenic acid (20:3n-6) proportions. The results suggest simultaneous pro-inflammatory and resolving actions of FAs and a potential role for EVs as vehicles for lipid mediators in asthma pathogenesis. EV lipid manifestations of EA can offer translational targets to study asthma pathophysiology and treatment options.
Revisiting and updating molecular epidemiology of α-thalassemia mutations in Thailand using MLPA and new multiplex gap-PCR for nine α-thalassemia deletion
Authors: Jomoui Wittaya , Panyasai Sitthichai , Sripornsawan Pornpun , Tepakhan Wanicha 
Published: June 2023

Abstractα-thalassemia is an inherited blood disorder that is most frequently found in Southeast Asian populations. In Thailand, molecular characterization can diagnose most patients with α-thalassemia; however, several atypical patients are also observed in routine analyses. Here, we characterized α-thalassemia mutations among 137 Hemoglobin H (Hb H) disease patients and three fetuses of Hb Bart’s hydrops, a fatal clinical phenotype of α-thalassemia. Specifically, we performed multiplex ligation-dependent probe amplification (MLPA) followed by direct DNA sequencing. We noticed common genotypes in 129 patients and eight patients had rare Hb H disease caused by compound heterozygous α0-thalassemia (--CR or --SA deletion) with α+-thalassemia (-α3.7/-α4.2/αConstant Springα). Furthermore, two affected fetuses had the --SA/--SEA and one had the --CR/--SEA genotypes. Next, we developed and validated a new multiplex gap-PCR and applied this method to 844 subjects with microcytic red blood cells (RBCs) from various parts of Thailand. The frequency of heterozygous α0-thalassemia was dominated by --SEA 363/844 (43%), followed by --THAI 3/844 (0.4%), --SA 2/844 (0.2%), and --CR 2/844 (0.2%) mutations. These findings suggest that aforementioned four mutations should be routinely applied to increase the effectiveness of diagnosis and genetic counseling in this region.
Investigating the role of Kinesin family in lung adenocarcinoma via integrated bioinformatics approach
Authors: Tabassum Gulnaz , Singh Prithvi , Gurung Rishabh , Hakami Mohammed Ageeli , Alkhorayef Nada , Alsaiari Ahad Amer , Alqahtani Leena S. , Hasan Mohammad Raghibul , Rashid Summya , Kumar Atul , Dev Kapil , Dohare Ravins 
Published: June 2023

AbstractLung cancer is the leading cause of mortality from cancer worldwide. Lung adenocarcinoma (LUAD) is a type of non-small cell lung cancer (NSCLC) with highest prevalence. Kinesins a class of motor proteins are shown to be involved in carcinogenesis. We conducted expression, stage plot and survival analyses on kinesin superfamily (KIF) and scrutinized the key prognostic kinesins. Genomic alterations of these kinesins were studied thereafter via cBioPortal. A protein–protein interaction network (PPIN) of selected kinesins and 50 closest altering genes was constructed followed by gene ontology (GO) term and pathway enrichment analyses. Multivariate survival analysis based on CpG methylation of selected kinesins was performed. Lastly, we conducted tumor immune infiltration analysis. Our results found KIF11/15/18B/20A/2C/4A/C1 to be significantly upregulated and correlated with poor survival in LUAD patients. These genes also showed to be highly associated with cell cycle. Out of our seven selected kinesins, KIFC1 showed the highest genomic alteration with highest number of CpG methylation. Also, CpG island (CGI) cg24827036 was discovered to be linked to LUAD prognosis. Therefore, we deduced that reducing the expression of KIFC1 could be a feasible treatment strategy and that it can be a wonderful individual prognostic biomarker. CGI cg24827036 can also be used as a therapy site in addition to being a great prognostic biomarker.
Taxonomic and functional diversity of land snails reflects habitat complexity in riparian forests
Authors: Gheoca Voichița , Benedek Ana Maria , Schneider Erika 
Published: June 2023

AbstractHabitat complexity affects the structure and dynamics of ecological communities, more often with increased complexity leading to greater species diversity and abundance. Among the terrestrial invertebrate groups, the low vagility of land snails makes them susceptible to react to small-scale habitat alteration. In the current paper we aimed to assess the relationship between taxonomic and functional composition and diversity of land snail communities and habitat structure in the riparian forest habitat. We found that both snail abundance and species richness responded positively to the increase in habitat complexity. The complexity of the riparian forest affected also the snail trait composition. Forest species, species living in woody debris, leaf litter, and root zone and those feeding on detritus were more abundant in complex habitats, while large snails with more offspring, snails having the ability to survive longer periods of dryness, as well as species that prefer arid habitats, were more abundant in less complex habitats. We concluded that habitat complexity promoted functional diversity, with the amount of woody debris as main positive driver, and the adjacent agricultural fields as negative driver of functional diversity.