bluuebunny/crossref_metadata_2025_split huggingface dataset:
The evolutionary history ofDrosophila simulansY chromosomes reveals molecular signatures of resistance to sex ratio meiotic drive
Authors: Courret C , Ogereau D , Gilbert C , Larracuente A.M , Montchamp-Moreau C 
Published: December 2022

ABSTRACTThe recent evolutionary history of the Y chromosome inDrosophila simulans, a worldwide species of Afrotropical origin, is closely linked to that of X-linked meiotic drivers (Paris system). The spread of the Paris drivers in natural populations has elicited the selection of drive resistant Y chromosomes. To infer the evolutionary history of the Y chromosome in relation to the Paris drive, we sequenced 21 iso-Y lines, each carrying a Y chromosome from a different location. Among them, 13 lines carry a Y chromosome that is able to counteract the effect of the drivers. Despite their very different geographical origins, all sensitive Y’s are highly similar, suggesting that they share a recent common ancestor. The resistant Y chromosomes are more divergent and segregate in four distinct clusters. The phylogeny of the Y chromosome confirms that the resistant lineage predates the emergence of Paris drive. The ancestry of the resistant lineage is further supported by the examination of Y-linked sequences in the sister species ofD. simulans, D. sechellia,andD. mauritiana. We also characterized the variation in repeat content among Y chromosomes and identified multiple simple satellites associated with resistance. Altogether, the molecular polymorphism allows us to infer the demographic and evolutionary history of the Y chromosome and provides new insights on the genetic basis of resistance.
A high-throughput sequencing approach identifies immunotherapeutic targets for bacterial meningitis in neonates
Authors: Pons Stéphanie , Frapy Eric , Sereme Youssouf , Gaultier Charlotte , Lebreton François , Kropec Andrea , Danilchanka Olga , Schlemmer Laura , Schrimpf Cécile , Allain Margaux , Angoulvant François , Lecuyer Hervé , Bonacorsi Stéphane , Aschard Hugues , Sokol Harry , Cywes-Bentley Colette , Mekalanos John J. , Guillard Thomas , Pier Gerald B. , Roux Damien , Skurnik David 
Published: December 2022

AbstractBackgroundWorldwide,Escherichia coliis the leading cause of neonatal Gram-negative bacterial meningitis, but full understanding of the pathogenesis of this disease is not yet achieved. Moreover, to date, no vaccine is available against bacterial neonatal meningitis.MethodsHere, we used Transposon Sequencing of saturated banks of mutants (TnSeq) to evaluateE. coliK1 genetic fitness in murine neonatal meningitis. We identifiedE. coliK1 genes encoding for factors important for systemic dissemination and brain infection, and focused on products with a likely outer-membrane or extra-cellular localization, as these are potential vaccine candidates. We usedin vitroandin vivomodels to study the efficacy of active and passive immunization.ResultsWe selected for further study the conserved surface polysaccharide Poly-β-(1-6)-N-Acetyl Glucosamine (PNAG), as a strong candidate for vaccine development. We found that PNAG was a virulence factor in our animal model. We showed that both passive and active immunization successfully prevented and/or treated meningitis caused byE. coliK1 in neonatal mice. We found an excellent opsonophagocytic killing activity of the antibodies to PNAG andin vitrothese antibodies were also able to decrease binding, invasion and crossing ofE. coliK1 through two blood brain barrier cell lines. Finally, to reinforce the potential of PNAG as a vaccine candidate in bacterial neonatal meningitis, we demonstrated that Group BStreptococcus, the main cause of neonatal meningitis in developed countries, also produced PNAG and that antibodies to PNAG could protectin vitroandin vivoagainst this major neonatal pathogen.InterpretationAltogether, these results indicate the utility of a high-throughput DNA sequencing method to identify potential immunotherapy targets for a pathogen, including in this study a potential broad-spectrum target for prevention of neonatal bacterial infections.FundingsANR Seq-N-Vaq, Charles Hood Foundation, Hearst Foundation. Groupe Pasteur Mutualité
Deleterious mutations in a heterozygous chromosome result in recessive lethality in silkworm semiconsomic strains
Authors: Tomihara Kenta , Tanaka Saori , Katsuma Susumu , Shimada Toru , Kobayashi Jun , Kiuchi Takashi 
Published: December 2022

AbstractIn this study, we found two embryonic lethal mutations,t04 lethal(l-t04) andm04 lethal(l-m04), in semiconsomic strains T04 and M04, respectively. In these semiconsomic strains, the entire diploid genome, except for one chromosome 4 of the wild silkwormBombyx mandarina, is substituted with chromosomes of the domesticated silkwormB. mori, andl-t04andl-m04mutations are located onB. mandarina-derivedchromosome 4. The mutations responsible for thel-t04andl-m04were identified as theBombyxhomolog ofimaginal discs arrested (Bmida) andTATA Box Binding Protein-associated factor 5 (BmTaf5), respectively. These findings indicate that both mutations were independently introduced during or after the development of semiconsomic strains. We conclude that the recessive embryonic lethality in the T04 and M04 strains is due to deleterious mutations produced inB. mandarina-derived chromosome 4.Graphical abstract
An electrostatic cluster guides Aβ40 fibril formation in cerebral amyloid angiopathy
Authors: Crooks Elliot J. , Fu Ziao , Irizarry Brandon A. , Zhu Xiaoyue , Van Nostrand William E. , Chowdhury Saikat , Smith Steven O. 
Published: December 2022

ABSTRACTCerebral amyloid angiopathy (CAA) is associated with the accumulation of fibrillar Aβ peptides upon and within the cerebral vasculature, which leads to loss of vascular integrity and contributes to disease progression in Alzheimer’s disease (AD). We investigate the structure of human-derived Aβ40 fibrils obtained from patients diagnosed with sporadic or familial Dutch-type (E22Q) CAA. Using cryo-EM, two primary structures are identified containing elements that have not been observed in in vitro Aβ40 fibril structures. One population has an ordered N-terminal fold comprised of two β-strands stabilized by electrostatic interactions involving D1, E22, D23 and K28. This charged cluster is disrupted in the second population, which exhibits a disordered N-terminus and is favored in fibrils derived from the familial Dutch-type CAA patient. These results illustrate differences between human-derived CAA and AD fibrils, and how familial CAA mutations guide fibril formation.
The inhibitory mechanism of a small protein reveals its role in antimicrobial peptide sensing
Authors: Jiang Shan , Steup Lydia C. , Kippnich Charlotte , Lazaridi Symela , Malengo Gabriele , Lemmin Thomas , Yuan Jing 
Published: December 2022

AbstractA large number of small membrane proteins have been discovered in bacteria, but their mechanism of action has remained mostly elusive. Here, we investigate the mechanism of a physiologically important small protein, MgrB, which represses the activity of the sensor kinase PhoQ and is widely distributed among enterobacteria. The PhoQ/PhoP two-component system is a master regulator of the bacterial virulence program and interacts with MgrB to modulate bacterial virulence, fitness, and drug resistance. A combination of crosslinking approaches with functional assays and protein dynamic simulations revealed structural rearrangements due to interactions between MgrB and PhoQ near the membrane/periplasm interface and along the transmembrane helices. These interactions induce the movement of the PhoQ catalytic domain and the repression of its activity. Without MgrB, PhoQ appears to be much less sensitive to antimicrobial peptides, including the commonly used C18G. In the presence of MgrB, C18G promotes MgrB to dissociate from PhoQ, thus activating PhoQ via derepression. Our findings reveal the inhibitory mechanism of the small protein MgrB and uncover its importance in antimicrobial peptide sensing.Significance StatementSmall proteins have high prevalence, vast diversity, and primarily regulatory functions in biological processes across all domains of life. However, their mechanisms of action remain largely elusive. In this study, we investigate the mechanism of the small protein, MgrB. It interacts with the sensor kinase PhoQ, rearranges its conformation, represses its kinase activity, and regulates bacterial response to environmental changes. In particular for antimicrobial peptides, MgrB is required for bacteria to have a selective response to this host-exclusive stimulus. Our findings underline the importance of a small protein in bacterial fitness and drug resistance and provide a molecular basis for engineering novel peptide-based regulators.
Gender construction and the invisibility of women’s accounting activities: the All Nations’ Fair of 1895
Authors: Halabi Abdel K. , Miley Frances , Read Andrew F. 
Published: December 2022

PurposeThis research explores the historical nexus between accounting and gender to illuminate male hegemonies within accounting. It examines the nature of that hegemony at the boundary between the female domain of household and philanthropic activities and the male domain of business and finance.Design/methodology/approachA qualitative research approach is used for this historical research. The primary source was digitised newspapers from the National Library of Australia. Newspapers have been used in previous historical accounting research and are relevant in this instance because they provide the only surviving data about the All Nations’ Fair. Given that newspapers were published daily, the depth of coverage is not replicated by other archival sources, and at that time provided a strong community voice.FindingsWomen undertook the management of and accounting for the All Nations’ Fair, a philanthropic activity designed to rescue the Geelong Cricket and Football Club from its parlous financial position. Despite women undertaking the work, the management of and accounting for, the Fair was attributed to men. This reflects a gendered construction of accounting that overpowers the reality of who undertook the work.Research limitations/implicationsThis research demonstrates only a single example of women’s philanthropic accounting, so is not generalisable. It suggests however that male hegemonies have exerted and continue to exert power over women.Originality/valueThe value of this paper is that historical examples serve as a corrective to histories that have ignored women’s contribution to accounting, particularly in philanthropic activities. The relationship between women’s accounting and gender also has contemporary significance. Gendered disadvantage and subjugation to a dominant masculine hegemony remain recurring themes in accounting research because they continue to impact adversely on the experiences of many women in accounting.
The pros and cons of reshoring to address the problems of shortages in global pharmaceutical value chains: the case of antibiotics
Authors: Baraldi Enrico , Ciabuschi Francesco , Fratocchi Luciano 
Published: December 2022

PurposeAntibiotics shortages have become an increasingly common problem in Europe because of several reasons, including the offshoring of the production of active pharmaceutical ingredients for many of these products to low production cost countries, such as China and India. The problem has deteriorated because of the Covid-19 crisis that has put most global value chains (GVCs) under great stress. This situation has boosted extensive discussions among academics, practitioners and policymakers on possible changes to the configuration of GVCs. This paper aims to focus specifically on antibiotics supply chains from the perspective of a small country (Sweden), and analyse the pros and cons of backshoring and nearshoring alternatives, as a means to reduce drug shortages.Design/methodology/approachThis work adopts a systemic perspective to capture the implications of reshoring for the different stakeholders involved in the antibiotics field. The present meso-analysis, focusing at the industry level, is based on multiple sources of primary data collected between 2014 and 2021, including participation in policy-related projects and interviews with over 100 representatives of key stakeholders in the antibiotics field.FindingsThis paper shows how reshoring can address the problems of drug shortages and reduce availability risk in antibiotics’ GVCs. However, the authors show that no simple and best solution exists because both alternatives of reshoring, i.e. backshoring and nearshoring, entail pros and cons for different stakeholders. The authors conclude with implications for policymakers and managers.Research limitations/implicationsThe analysis of pros and cons of both backshoring and nearshoring for various stakeholders offers relevant implications for research on operations and supply management, international business and economics/political science.Originality/valueThis paper looks at reshoring as a policy-driven decision and provides an innovative systemic perspective to analyse the implications for different stakeholders of two reshoring options concerning the antibiotics supply chain.
Investigation of deinking efficiencies of trigromi laserjet printed papers depending on the number of recycling
Authors: Yılmaz Ufuk 
Published: December 2022

Purpose
This study aims to determine the ink removal efficiency of papers with different recycling numbers and to examine some electrophotographic printing properties.

Design/methodology/approach
The base papers prepared according to the INGEDE 11p standard are subjected to six recycling stages (RS) under equal conditions. The physical-optical properties of the papers obtained at the end of each RS are measured and CMYK (cyan, magenta, yellow, key) color measurement scales are printed on each paper with electrophotographic printing. Color measurements of the printed papers are measured using the X-Rite eXact spectrophotometer, adhering to the ISO 13655:2017 standard. According to the measurement results of the optical properties, the ink removal efficiency of each recycling step is determined as a percentage (%) using some formulas.

Findings
As general, according to DEMLab and IERIC data, it is determined that the ink removal efficiency increased as the recycling repetition increased. In DEMf factor values, the highest deinking efficiency is obtained after the fourth RS. There is no significant difference between the printing properties of the samples.

Originality/value
It has been a matter of curiosity that papers lose their properties after how many RS. Many studies have been carried out on this subject and it has been presented by experimental methods that the printability properties of papers increase or decrease after which RS. This study can be a pioneer for future studies.
Precision cellular agriculture: The future role of recombinantly expressed protein as food
Authors: Dupuis John H. , Cheung Lennie K.Y. , Newman Lenore , Dee Derek R. , Yada Rickey Y. 
Published: December 2022

AbstractCellular agriculture is a rapidly emerging field, within which cultured meat has attracted the majority of media attention in recent years. An equally promising area of cellular agriculture, and one that has produced far more actual food ingredients that have been incorporated into commercially available products, is the use of cellular hosts to produce soluble proteins, herein referred to as precision cellular agriculture (PCAg). In PCAg, specific animal‐ or plant‐sourced proteins are expressed recombinantly in unicellular hosts—the majority of which are yeast—and harvested for food use. The numerous advantages of PCAg over traditional agriculture, including a smaller carbon footprint and more consistent products, have led to extensive research on its utility. This review is the first to survey proteins currently being expressed using PCAg for food purposes. A growing number of viable expression hosts and recent advances for increased protein yields and process optimization have led to its application for producing milk, egg, and muscle proteins; plant hemoglobin; sweet‐tasting plant proteins; and ice‐binding proteins. Current knowledge gaps present research opportunities for optimizing expression hosts, tailoring posttranslational modifications, and expanding the scope of proteins produced. Considerations for the expansion of PCAg and its implications on food regulation, society, ethics, and the environment are also discussed. Considering the current trajectory of PCAg, food proteins from any biological source can likely be expressed recombinantly and used as purified food ingredients to create novel and tailored food products.
Quantifying exposure to chlorhexidine from decontamination of peripheral intravenous injection ports
Authors: Doane Matthew A. , Kwong Christopher , Proschogo Nicholas 
Published: December 2022

AbstractBackgroundAnnually, almost 2 billion peripheral intravenous cannulas (PIVCs) are placed worldwide. In response to concerns of infectious complications, chlorhexidine is increasingly utilised for maintenance decontamination of PIVC injection ports. Concomitantly, the allergenic potential of chlorhexidine exposures has been highlighted by several case reports, implicating it as a trigger when used for this seemingly innocuous process. Given how widespread this application is, elucidating potential chlorhexidine exposure is needed to gauge its risks and benefits.ObjectiveTo examine and quantify if chlorhexidine is entrained when used for PIVC injection port cleaning.MethodsTwenty benchtop PIVC set‐ups were cleaned with 2% chlorhexidine and 70% alcohol wipes, following three different pragmatic protocols. Each set‐up was injected with 10 ml ultrapure water, and samples tested by liquid chromatography‐electrospray tandem mass spectrometry for entrained chlorhexidine.ResultsChlorhexidine was detected in every sample. Mean concentrations and standard deviations from each protocol were 41.47 ppb (4.08), 54.76 ppb (17.46), and 65.84 ppb (7.01). One‐way ANOVA indicated a statistical difference between at least two groups (df = 2, F = 24.11, p < .00001), with Tukey's testing verifying significantly different mean concentrations between all groups (p < .01).ConclusionsUsing 2% chlorhexidine and 70% alcohol swabs to decontaminate PIVC injection ports resulted in consistent entrainment of chlorhexidine, with varying amounts correlated to how it was applied. These results validate case reports attributing anaphylactic/allergic reactions to suspected intravenous chlorhexidine entrainment and should factor into future risk–benefit assessments for its use in PIVC maintenance antisepsis policies and protocols.
Associations between alcohol and cannabis use order, frequency, quantity, and consequences in a college sample of individuals who co‐use alcohol and cannabis
Authors: Karoly Hollis C. , Conner Bradley T. , Prince Mark A. 
Published: December 2022

AbstractBackground and ObjectivesUsing both alcohol and cannabis (either at the same time or at different times) is common among college students, and is called “co‐use.” Using these substances simultaneously, such that their effects overlap, is thought to be an especially risky co‐use pattern. Gaining a better understanding of how co‐use patterns relate to substance use and consequences could aid prevention and intervention efforts.MethodsWe examined college students (N = 401) who reported using both alcohol and cannabis at least once in the past 30 days. Path analysis was used to explore relations among co‐use patterns (number of days in a typical week that participants used both alcohol and cannabis; the number of days using alcohol first, cannabis first, alcohol last, and cannabis last; the number of days of simultaneous use), past‐30‐day alcohol and cannabis consequences, use frequency, and typical quantities used.ResultsEach additional day of using alcohol first was associated with fewer past‐30‐day cannabis consequences. Each additional day of using cannabis first was associated with fewer alcohol‐related consequences. Each additional day of using alcohol and cannabis on the same day and each additional day of simultaneous use were both associated with less cannabis used and alcohol consumed in a typical week.Conclusions and Scientific SignificanceThis study is among the first to identify associations between alcohol and cannabis order and outcomes (i.e., consequences and consumption). Results suggest that modifying which substance is used first on a given day could be a practical intervention strategy for individuals who co‐use alcohol and cannabis.
Tacrolimus prevents complement‐mediated Nod‐like receptor family pyrin domain containing 3 (NLRP3) inflammasome activation and pyroptosis of mesenchymal stem cells from immune thrombocytopenia
Authors: Cai Xuan , Wu Jin , An Zhuo‐Yu , Wang Chen‐Cong , Zhu Xiao‐Lu , He Yun , Fu Hai‐Xia , Huang Xiao‐Jun , Zhang Xiao‐Hui 
Published: December 2022

SummaryThe abnormal immunomodulatory functions of mesenchymal stem cells (MSCs) have been implicated in the development of immune thrombocytopenia (ITP). Recent studies have suggested important effects of complement on immune cell function. However, whether complement modulates bone marrow MSCs function in ITP is poorly defined. Tacrolimus has recently been applied to the treatment of autoimmune diseases. Here, we explored whether impaired ITP‐MSCs could be targeted by tacrolimus. Our results showed that the Nod‐like receptor family pyrin domain containing 3 (NLRP3) inflammasome was activated in ITP MSCs with complement deposition (MSCs‐C+) and initiated caspase‐1‐dependent pyroptosis. Transcriptome sequencing results showed abnormal fatty acid metabolism in MSCs‐C+. Enhanced fatty acid β‐oxidation and reactive oxygen species production activated the NLRP3 inflammasome. Adipocytes derived from MSCs‐C+ secreted less adiponectin. Adiponectin promoted the differentiation of megakaryocytes and inhibited the destruction of platelets. Tacrolimus inhibited NLRP3 inflammasome activation and MSCs‐C+ pyroptosis in vitro and in vivo. Tacrolimus plus danazol elicited a higher sustained response than danazol monotherapy in corticosteroid‐resistant patients with ITP. Our findings demonstrate that the activation of the NLRP3 inflammasome in ITP MSCs mediated by complement could be inhibited by tacrolimus, which might be a potential new therapy for ITP.