bluuebunny/crossref_metadata_2025_split huggingface dataset:
Presence of an ultra-small microbiome in fermented cabbages
Authors: Lee Hae-Won , Yoon So-Ra , Dang Yun-Mi , Kang Miran , Lee Kwangho , Ha Ji-Hyung , Bae Jin-Woo 
Published: July 2023

Background
Ultramicrobacteria (UMB), also known as ultra-small bacteria, are tiny bacteria with a size less than 0.1 µm3. They have a high surface-to-volume ratio and are found in various ecosystems, including the human body. UMB can be classified into two types: one formed through cell contraction and the other that maintains a small size. The ultra-small microbiome (USM), which may contain UMB, includes all bacteria less than 0.2 µm in size and is difficult to detect with current methods. However, it poses a potential threat to food hygiene, as it can pass through sterilization filters and exist in a viable but non-culturable (VBNC) state. The data on the USM of foods is limited. Some bacteria, including pathogenic species, are capable of forming UMB under harsh conditions, making it difficult to detect them through conventional culture techniques.

Methods
The study described above focused on exploring the diversity of USM in fermented cabbage samples from three different countries (South Korea, China, and Germany). The samples of fermented cabbage (kimchi, suancai, and sauerkraut) were purchased and stored in chilled conditions at approximately 4 °C until filtration. The filtration process involved two steps of tangential flow filtration (TFF) using TFF cartridges with different pore sizes (0.2 µm and 100 kDa) to separate normal size bacteria (NM) and USM. The USM and NM isolated via TFF were stored in a refrigerator at 4 °C until DNA extraction. The extracted DNA was then amplified using PCR and the full-length 16S rRNA gene was sequenced using single-molecule-real-time (SMRT) sequencing. The transmission electron microscope (TEM) was used to confirm the presence of microorganisms in the USM of fermented cabbage samples.

Results
To the best of our knowledge, this is the first study to identify the differences between USM and NM in fermented cabbages. Although the size of the USM (average 2,171,621 bp) was smaller than that of the NM (average 15,727,282 bp), diversity in USM (average H′ = 1.32) was not lower than that in NM (average H′ = 1.22). In addition, some members in USM probably underwent cell shrinkage due to unfavorable environments, while others maintained their size. Major pathogens were not detected in the USM in fermented cabbages. Nevertheless, several potentially suspicious strains (genera Cellulomonas and Ralstonia) were detected. Our method can be used to screen food materials for the presence of USM undetectable via conventional methods. USM and NM were efficiently separated using tangential flow filtration and analyzed via single-molecule real-time sequencing. The USM of fermented vegetables exhibited differences in size, diversity, and composition compared with the conventional microbiome. This study could provide new insights into the ultra-small ecosystem in fermented foods, including fermented cabbages.
Study of the Hydroxymethylfurfural and Sugar Profile of Apis melifera Honey in Madhya Pradesh, India
Authors: Saxena Sakshi , Yadav A. S. , Tomar S. P. S. 
Published: July 2023

Aims: The present study was undertaken to determine the Hydroxymethylfurfural and sugar profile of Apis melifera honey.
Place and Duration of Study: Honey samples were collected from beekeepers of eight selected villages of Guna and Morena region of Madhya Pradesh during two season 2021/22 - 2022/23
Methodology: The study covers the sugar characterization in term of reducing sugar, sucrose, glucose, fructose, fructose/glucose ratio and Hydroxymethylfurfural content. Collected honey samples were investigated in lab and data analysis by two factor without replication.
Results: The result recorded different parameters Hydroxymethylfurfural, (9.77-15.73 and 9.50- 15.91 mg/kg) total reducing sugars (70.08-73.53 and 69.16-73.83 %), sucrose (1.70-3.43 and 1.77-3.39%), glucose (31.60-35.60 and 31.20-35.30), fructose (36.47- 39.94 and 36.73-.39.69) and
fructose glucose ratio (1.03-1.22 and 1.04-1.22) respectively, during 2021-22 and 2022-23. All the parameters showed significant variation in honey among the selected locations.
Conclusion: According to the study, all of the honey samples met the allowed limit as required by Indian standards and were of acceptable quality.
The Effect of Hydrocortisone-Ascorbic Acid and Thiamine Therapy on the Outcome of Patients with Sepsis: Prospective Randomized Double-Blinded Controlled Trial
Authors: Sharaf Mostafa Ismail , Ismaeil Sameh Abd El Khalik , Yazeed Mohamed Mohi El Deen Abo El , El-Gendy Hala Mohey El-Deen , Alsherif Salah Eldeen Ibrahim 
Published: July 2023

Background: Millions of individuals worldwide suffer from septic shock and sepsis each year, with fatalities reaching one in four and often higher. This work was conducted to evaluate the impact of Ascorbic acid, hydrocortisone, and thiamine treatment on the 28th day mortality rate and the outcomes (changes in SOFA score, incidence of organ dysfunction, alterations in serum procalcitonin level, changes in serum lactate level and total dose of vasopressor therapy.
Methods: This prospective randomised controlled double-blinded work was performed on 80 individuals ranging in age from 18 to 65 years old and presented with sepsis who were diagnosed based on the SSC 2016 and The SSC Bundle 2018 Update. The participants were divided into two groups at random. Group I (placebo): The conventional approach was used to treat those who were in this group. The participants were given 50 ml of normal saline intravenously within 30 minutes, 5 ml intravenously within 6 hours, and 10 ml intravenously within 12 hours. Group II: the participants had standard sepsis treatment as well as a combination of ascorbic acid 1.5 gm diluted in 50 ml of normal saline IV within 30 minutes of 6 hours and thiamine 200 mg diluted in 10 ml of normal saline IV over a 12-hour period. If the admission term was shorter than 4 days, this combination treatment was provided until the time of release for a total of 4 days.
Results: No statistically substantial variation was existed among both groups as regard incidence of 28th day mortality and incidence of organ dysfunction. At day one, the comparison of the mean values of mean arterial pressure (MAP) and cardiac index was comparable between the two groups. At day 2,3 and 4, the mean values of MAP were greater in group II in contrast to group I while cardiac index was greater in group I compared to group II. The mean value (± SD) of the total dose of noradrenaline was 824.1 ± 296.8 mg in group I, and 591.9 ± 343.2 mg in group II, with statistically substantial variation among the two groups (P<0.001)
Conclusions: The combined use of ascorbic acid, thiamine, and hydrocortisone has no impact on the 28th day mortality and organ dysfunction among individuals with septic shock and sepsis but has a good impact on haemodynamic parameters and inflammatory markers during the period of drug administration.
Effect of soil type and sowing depth on the germination and early growth of two grain amaranth cultivars
Authors: Gomes Victor E. de V. , Lindsey Laura E. , Mesquita Rosilene O. 
Published: July 2023

AbstractGrain amaranth is a highly nutritious pseudo‐cereal with good adaptability to a range of environmental conditions and potential to be further cultivated in many areas of the world. Although the crop has good adaptability, soil type can influence crop establishment and production; thus, the objective of this study was to evaluate how cultivar, sowing depth, and soil type affect emergence and early growth of grain amaranth. A pot experiment was carried out in two runs under greenhouse conditions. The experiment was set in a randomized complete block design in a 3 × 5 × 2 factorial arrangement with three soil types (loam, clay loam, and clay) × five sowing depths (0, 5, 10, 15, and 20 mm) × two grain amaranth cultivars (Amaranthus cruentus BRS Alegria and Amaranthus caudatus Inca). Amaranthus caudatus had greater seedling height, stem diameter, number of leaves, root length, fresh weight, and dry weight compared to A. cruentus. Emergence was affected by cultivar and was 15% greater for A. cruentus. Seedling height was significantly greater at a 5 and 20 mm sowing depth. Root growth varied as a function of the interaction between soil types and sowing depths in which the longest roots were obtained when grain amaranth was sown in a loam soil at 10 mm depth. For better growth of grain amaranth, the recommended combination is A. caudatus in a loam soil at a depth between 5 and 20 mm.
Genetic liability for gastrointestinal inflammation disorders and association with gastrointestinal symptoms in children with and without autism
Authors: Morrill Valerie , Benke Kelly , Brinton John , Soke Gnakub N. , Schieve Laura A. , Fields Victoria , Farzadegan Homayoon , Holingue Calliope , Newschaffer Craig J. , Reynolds Ann M. , Fallin M. Daniele , Ladd‐Acosta Christine 
Published: July 2023

AbstractChildren with autism spectrum disorder (ASD) have a greater prevalence of gastrointestinal (GI) symptoms than children without ASD. We tested whether polygenic scores for each of three GI disorders (ulcerative colitis, inflammatory bowel disease, and Crohn's disease) were related to GI symptoms in children with and without ASD. Using genotyping data (564 ASD cases and 715 controls) and external genome‐wide association study summary statistics, we computed GI polygenic scores for ulcerative colitis (UC‐PGS), inflammatory bowel disease (IDB‐PGS), and Crohn's disease (CD‐PGS). Multivariable logistic regression models, adjusted for genetic ancestry, were used to estimate associations between each GI‐PGS and (1) ASD case–control status, and (2) specific GI symptoms in neurotypical children and separately in ASD children. In children without ASD, polygenic scores for ulcerative colitis were significantly associated with experiencing any GI symptom (adjusted odds ratio (aOR) = 1.36, 95% confidence interval (CI) = 1.03–1.81, p = 0.03) and diarrhea specifically (aOR = 5.35, 95% CI = 1.77–26.20, p = 0.01). Among children without ASD, IBD‐PGS, and Crohn's PGS were significantly associated with diarrhea (aOR = 3.55, 95% CI = 1.25–12.34, p = 0.02) and loose stools alternating with constipation (aOR = 2.57, 95% CI = 1.13–6.55, p = 0.03), respectively. However, the three PGS were not associated with GI symptoms in the ASD case group. Furthermore, polygenic scores for ulcerative colitis significantly interacted with ASD status on presentation of any GI symptom within a European ancestry subset (aOR = 0.42, 95% CI = 0.19–0.88, p = 0.02). Genetic risk factors for some GI symptoms differ between children with and without ASD. Furthermore, our finding that increased genetic risks for GI inflammatory disorders are associated with GI symptoms in children without ASD informs future work on the early detection of GI disorders.
A Targeting Singlet Oxygen Battery for Multidrug‐Resistant Bacterial Deep‐Tissue Infections
Authors: Zhu Yiwen , Gao Minzheng , Su Mengrui , Shen Yanzhe , Zhang Kai , Yu Bingran , Xu Fu‐Jian 
Published: July 2023

AbstractTraditional photodynamic therapy (PDT) is dependent on externally applied light and oxygen, and the depth of penetration of these factors can be insufficient for the treatment of deep infections. The short half‐life and short diffusion distance of reactive oxygen species (ROS) also limit the antibacterial efficiency of PDT. Herein, we designed a targeting singlet oxygen delivery system, CARG‐Py, for irradiation‐free and oxygen‐free PDT. This system was converted to the “singlet oxygen battery” CARG‐1O2 and released singlet oxygen without external irradiation or oxygen. CARG‐1O2 is composed of pyridones coupled to a targeting peptide that improves the utilization of singlet oxygen in deep multidrug‐resistant bacterial infections. CARG‐1O2 was shown to damage DNA, protein, and membranes by increasing the level of reactive oxygen inside bacteria; the attacking of multiple biomolecular sites caused the death of methicillin‐resistant Staphylococcus aureus (MRSA). An in vivo study in a MRSA‐infected mouse model of pneumonia demonstrated the potential of CARG‐1O2 for the efficient treatment of deep infections. This work provides a new strategy to improve traditional PDT for irradiation‐ and oxygen‐free treatment of deep infections while improving convenience of PDT.
From Tethered to Freestanding Stabilizers of 14‐3‐3 Protein‐Protein Interactions through Fragment Linking
Authors: Visser Emira J. , Jaishankar Priyadarshini , Sijbesma Eline , Pennings Marloes A. M. , Vandenboorn Edmee M. F. , Guillory Xavier , Neitz R. Jeffrey , Morrow John , Dutta Shubhankar , Renslo Adam R. , Brunsveld Luc , Arkin Michelle R. , Ottmann Christian 
Published: July 2023

AbstractSmall‐molecule stabilization of protein‐protein interactions (PPIs) is a promising strategy in chemical biology and drug discovery. However, the systematic discovery of PPI stabilizers remains a largely unmet challenge. Herein we report a fragment‐linking approach targeting the interface of 14‐3‐3 and a peptide derived from the estrogen receptor alpha (ERα) protein. Two classes of fragments—a covalent and a noncovalent fragment—were co‐crystallized and subsequently linked, resulting in a noncovalent hybrid molecule in which the original fragment interactions were largely conserved. Supported by 20 crystal structures, this initial hybrid molecule was further optimized, resulting in selective, 25‐fold stabilization of the 14‐3‐3/ERα interaction. The high‐resolution structures of both the single fragments, their co‐crystal structures and those of the linked fragments document a feasible strategy to develop orthosteric PPI stabilizers by linking to an initial tethered fragment.
Molecular dynamics coupled with experimental study of the effects of polyethylene glycol on the structure and antifouling properties of polyethersulfone membranes
Authors: Luo Yang , Liu Zihan , Liu Yi , Huang Jiaxun , Zhang Ming 
Published: July 2023

AbstractPolyethylene glycol (PEG), as a hydrophilic molecule, exerts a significant influence on the morphology and performance of membranes. However, the mechanism by which PEG influences polyethersulfone (PES) membranes remains unclear. In this work, the addition of varying concentrations (4, 12, 24 wt%) and molecular weights (200, 400, and 600 Da) of PEG on the PES membrane was investigated by molecular dynamics (MD) simulation and experimental study. Compared to the concentration of PEG (4, 12, and 24 wt%), the molecular weight of PEG (PEG200, PEG400, and PEG600) had little effect on the membrane pore size. The radial distribution function and fractional free volume results of MD simulation both proved that. Additionally, both the experimental adsorption capacity (QHA) and the calculated adsorption energy (Eads) revealed that the adsorption of humic acid (HA) on the PES membrane with 4 wt% PEG was higher than that on the pure PES membrane, which meant that PEG was not beneficial to the anti‐fouling property but improved the water flux of PES membranes. The results of this study not only provide theoretical predictions and a scientific basis for the formulation of PES membranes but also promotes an approach for exploring the mechanism by which additives affect polymer membranes.
hybpiper‐nf and paragone‐nf: Containerization and additional options for target capture assembly and paralog resolution
Authors: Jackson Chris , McLay Todd , Schmidt‐Lebuhn Alexander N. 
Published: July 2023

AbstractPremiseThe HybPiper pipeline has become one of the most widely used tools for the assembly of target capture data for phylogenomic analysis. After the production of locus sequences and before phylogenetic analysis, the identification of paralogs is a critical step for ensuring the accurate inference of evolutionary relationships. Algorithmic approaches using gene tree topologies for the inference of ortholog groups are computationally efficient and broadly applicable to non‐model organisms, especially in the absence of a known species tree.Methods and ResultsWe containerized and expanded the functionality of both HybPiper and a pipeline for the inference of ortholog groups, providing novel options for the treatment of target capture sequence data, and allowing seamless use of the outputs of the former as inputs for the latter. The Singularity container presented here includes all dependencies, and the corresponding pipelines (hybpiper‐nf and paragone‐nf, respectively) are implemented via two Nextflow scripts for easier deployment and to vastly reduce the number of commands required for their use.ConclusionsThe hybpiper‐nf and paragone‐nf pipelines are easily installed and provide a user‐friendly experience and robust results to the phylogenetic community. They are used by the Australian Angiosperm Tree of Life project. The pipelines are available at https://github.com/chrisjackson-pellicle/hybpiper-nf and https://github.com/chrisjackson-pellicle/paragone-nf.
Regulating Zinc Deposition via Zincophilic 2D‐Cu2Te as the Current Collector to Suppress Dendrite Formation toward High Performance Aqueous Zinc‐Ion Batteries
Authors: Tsai Ming‐Hsuan , Lin Tuan‐Yue , Su Tian‐Shun , Chen Guan‐Min , Liu Yu‐chen , Chen Yu‐Ze 
Published: July 2023

AbstractInvited for this month's cover is the group of Nano Horizons Lab hosted by Prof. Yu‐Ze Chen. The cover picture shows the lateral deposition of Zn guided by zincophilic Cu2Te flakes, resulting in the suppression of dendrite growth. Read the full text of the Research Article at 10.1002/batt.202300107.
Detection and identification of imperatorin metabolites in rat, dog, monkey, and human liver microsomes by ultra‐high‐performance liquid chromatography combined with high‐resolution mass spectrometry and Compound Discoverer software
Authors: He Fan , Zeng Fenglian , Situ Xiaohong , He Runmin , Zheng Wei , Chen Yongzhuang , Ou Dinghong , Chen Yilu 
Published: July 2023

AbstractImperatorin, a furanocoumarin that widely exists in many umbelliferous herbs, has been demonstrated to have a variety of pharmacological effects, including anti‐inflammatory, antiosteoporosis, and antitumor activities. The purpose of this study was to investigate the metabolism of imperatorin using liver microsomes. The metabolites were generated by individually incubating imperatorin with rat, dog, monkey, and human liver microsomes. To trap the reactive metabolites during microsomal metabolism, glutathione (GSH) was included in the incubation. A LC technique coupled with benchtop orbitrap MS with full mass/data‐dependent tandem mass spectrometry acquisition mode was used to detect and identify the generated metabolites. The possible structures of the metabolites were characterized according to their accurate masses and fragment ions. Under the current conditions, a total of 10 metabolites, including four GSH adducts, were identified. The results indicated that imperatorin underwent extensive metabolic reactions including hydroxylation, oxidation, glucuronidation, and GSH conjugation. This study provides essential data on the metabolism of imperatorin, which will be helpful for us to understand the safety and efficacy of this bioactive compound.
Non‐sustainable buying behavior: How the fear of missing out drives purchase intentions in the fast fashion industry
Authors: Bläse Richard , Filser Matthias , Kraus Sascha , Puumalainen Kaisu , Moog Petra 
Published: July 2023

AbstractMovements like “Fridays for Future” have heightened attention to the need for sustainability, particularly among Generations X, Y, and Z. However, the consumption of fast fashion and so‐called ultra‐fashion products—an ecologically harmful business model—continues to gain momentum, especially among young consumers, not least due to fear of missing out (FOMO). FOMO is well‐known among marketing professionals as a strong trigger for frequently recurring buying behavior. Over the past 5 years, scholars have become increasingly interested in how FOMO triggers buying behavior and have begun to incorporate FOMO in their cognitive models. However, the influence of FOMO on individual fashion purchases and the relationship between brand credibility and sustainable fashion production is not yet well understood. Utilizing cross‐sectional data from three distinct samples in Switzerland and the United States, our study, which included over 650 participants, reveals that brand credibility and FOMO exert direct influences on consumers' purchase intentions for fast fashion products. We identify that FOMO has a negative moderating effect on the relationship between brand credibility and fast fashion purchase intentions. Suggesting that consumers with strong FOMO are less interested in brand credibility when making a purchase decision than those without FOMO. Additionally, we demonstrate that our findings apply to both fast and slow fashion, the latter encompassing sustainably produced fashion. Ultimately, we provide novel empirical evidence of FOMO's influence on buying behavior and shed light on the complex interplay between brand credibility, sustainability, and consumer behavior in the fashion industry.
Early cryptococcosis infection in a liver transplant patient: A case report
Authors: Sadeghi Boogar Shahrokh , Sivandzadeh Gholam Reza , Sehatpour Faezeh , Dadashpour Nazanin , Goodarzian MReza , Parvar Seyedeh Yasamin 
Published: July 2023

Key Clinical MessageIn order to early diagnose and prevent the infection dissemination in both suspected solid organ donors and recipients after transplantation, pretransplantation screening tests for rare etiologies like Cryptococcus neoformans should be necessitated, as they can affect many vital organs, especially the brain, liver, and lungs.AbstractCryptococcosis is a systemic fungal infection mainly affecting immunocompromised patients. The infection is occasionally seen in 16–21 months after organ transplantation, while early involvement is uncommon within <30 days posttransplantation. In the present study, we reported an unusual case of cryptococcosis infection 21 days after transplantation, limited to the transplanted liver in a 60‐year‐old male. Treatment with an antifungal agent showed prompt improvement in his clinical condition.
Hydrodynamics in a Bubble Column – Part 1: Two‐Phase Flow
Authors: Sommer Anna-Elisabeth , Draw Mazen , Wang Lantian , Schmidtpeter Jan , Hessenkemper Hendrik , Gatter Josefine , Nam Haein , Eckert Kerstin , Rzehak Roland 
Published: July 2023

AbstractMultiphase computational fluid dynamics (CFD) simulation is a useful tool to study the hydrodynamics in a bubble column if appropriate closure models are known. Systematic assessment of different models is an ongoing venture that benefits from improved validation data. The present study accumulates a database on two‐phase flow experiments in a bubble column. This is achieved by using a combination of particle image velocimetry and shadowgraphy to measure the liquid velocity field and gas dispersion properties simultaneously. This methodology is applied for different needle diameters and gas flow rates. The experimental data are compared with CFD simulations which show good predictions. A systematic investigation of the three‐phase flow in the bubble column will appear as a sequel.