bluuebunny/crossref_metadata_2025_split huggingface dataset:
The effects of pulse width of a direct current used in water bath stunning and of slaughter methods on spontaneous electroencephalograms in broilers
Authors: Raj ABM , O'Callaghan M , Hughes SI 
Published: January 2023

AbstractThe effect of the pulse width of a direct current (DC) on the effectiveness of electrical water bath stunning, and slaughter, was evaluated in broilers (n = 29). Broilers were individually stunned in a water bath for 1 s with a constant peak current of 400 mA of 200 Hz DC delivered using a variable voltage/constant current stunner. The pulse width of the 200 Hz DC was set at 0.5, 1.5 or 2.5 ms (10, 30 or 50% of 5 ms current cycle). The results showed that pulse width had a significant effect on the incidence of epileptiform activity in the electroencephalograms (EEGs). A pulse width of 10% of the current cycle was less effective than pulse widths of 30 and 50% of the current cycle; there was no significant difference between a pulse width of 30 and 50%. The results of a univariate analysis showed that ventral neck-cutting resulted in a significantly shorter time to the onset of less than 10% of the pre-stun power contents in the 13–30 Hz and 2–30 Hz EEG frequency bands when compared with unilateral neck-cutting. It is concluded that a pulse width of 30 or 50% of the current cycle of 200 Hz DC, delivering 400 mA peak current, was better than using a pulse width of 10% of the current cycle.
Immediate immobilisation of a Minke whale using a grenade harpoon requires striking a restricted target area
Authors: Knowles TG , Butterworth A 
Published: January 2023

AbstractApproximately 1500 Minke whales are killed annually under permit from the International Whaling Commission (IWC). This hunt supports a modest industry in Norway and in Japan; however, the welfare of whales during hunting and killing is such a cause of concern that in 1980 the IWC formed a sub-group entitled ‘Working Group on Whale Killing Methods and Associated Welfare Issues’ devoted to discussing the issue. This commentary suggests that, when using the Norwegian penthrite grenade-tipped harpoon (‘Whalegrenade-99‘), it is necessary to hit a relatively well-defined target area in order to effect an immediately immobile, and presumed unconscious state in the Minke whale.
A review of the welfare consequences of surgical castration in piglets and the evaluation of non-surgical methods
Authors: Prunier A , Bonneau M , von Borell EH , Cinotti S , Gunn M , Fredriksen B , Giersing M , Morton DB , Tuyttens FAM , Velarde A 
Published: January 2023

AbstractMale piglets are castrated primarily to prevent the unpleasant odours and flavours of entire male pig meat (boar-taint). Although castration can be legally performed without analgesia in the first seven days of life, available evidence shows that castration at any age is painful and may have a detrimental influence on health. Few anaesthetics or analgesics are licensed for use in piglets. The known methods for general and epidural anaesthesia cannot be run at the farm level for practical and/or legal reasons. Use of the local anaesthetic lidocaine is easy and allows the pain resulting from castration to be alleviated. Local destruction of testicular tissue by intra-testicular injection of chemical compounds (salts and acids) is an alternative to surgical castration but needs further investigation regarding welfare improvement and boar taint reduction. Immunocastration, by which castration is achieved using active immunisation (anti–GnRH immunisation) is an efficient alternative to surgical castration; however, there are no licensed vaccines in the EU and the consequences, in terms of pig welfare as well as its acceptability among EU consumers, need further evaluation.
The effect of cover on food consumption and growth in two freshwater fish species used in experimental studies
Authors: Wootton RJ , Handisyde N , Rowe C 
Published: January 2023

AbstractTo define suitable holding conditions for fish used in experimental studies on food consumption and growth, the effect of cover on growth at two ration levels was quantified for immature individuals of two common, freshwater species: the threespine stickleback (Gasterosteus aculeatus: Gasterosteidae) and the European minnow (Phoxinus phoxinus: Cyprinidae). In the European minnow, the presence of cover significantly increased growth rates and also the rate of food consumption atad libitumrations; however, cover had no significant effect on the growth of the threespine stickleback. In both species, fish fedad libitumrations had higher growth rates than fish fed 4% of body weight per day. This study illustrates the need for species specific holding conditions and illustrates an approach to welfare using measurements of traits closely related to fitness.
Effects of aviary and box sizes on body mass and behaviour of domesticated budgerigars (Melopsittacus undulatus)
Authors: Gebhardt-Henrich SG , Steiger A 
Published: January 2023

AbstractForty-eight budgerigars (Melopsittacus undulatus) were pairwise housed in small (80 × 40 × 50 cm) and large (160 × 40 × 50 cm) (length × breadth × height) boxes, as well as in 2.0 × 1.0 × 2.0 m aviaries. All boxes and aviaries had two or three (aviaries) perches and food was offered on the bottom. The body masses of female budgerigars increased significantly when they were housed in boxes instead of aviaries. The size of the box did not influence body mass. The frequency of flying was adjusted to the size of the box as both males and females flew approximately twice as often from perch to perch in a small box than in a large box of double length. Flying behaviour differed significantly between large and small boxes and aviaries. The ratio of invariantly flying from perch to perch to all flying events increased from aviaries < large boxes < small boxes. About 75% of all flying events in small boxes consisted of flying from perch to perch. Females in boxes spent more time on the bottom where the food was placed than females in aviaries. Both the significant body mass gain in female budgerigars, as well as the invariant, stereotypic flying movements, indicated reduced welfare in budgerigars housed in boxes, compared with those in aviaries. Providing a large box did not prevent body mass gain, but did increase the variation in flying patterns. Under long-term housing conditions, boxes could lead to at least two serious welfare problems; obesity and stereotypic behaviour, and should be avoided for budgerigars. Therefore, aviary housing should be chosen wherever possible.
Long-term social memory in the laboratory rat (Rattus norvegicus)
Authors: Burman OHP , Mendl M 
Published: January 2023

AbstractA key question in the management of group-housed captive animals is how long can an individual be removed from a social group and still be reintroduced with minimal social upheaval. In order to answer this question we require a knowledge of how long cage-mates, following a specified period of group-housing, can remember one another after separation. This issue was investigated in laboratory rats (Rattus norvegicus). Rats were group-housed for 18 days before being housed individually. One hour, 48 hr, and 96 hr after separation, they were exposed simultaneously to odour cues originating from unfamiliar rats and from former cage-mates. The rats spent significantly more time investigating the unfamiliar odour 1 hr and 48 hr, but not 96 hr, after separation, suggesting that, after 18 days of group-housing, juvenile rats remember former cage-mates for between 48 and 96 hr. The implications of this result for animal welfare are discussed.
Do double cages and access to occupational materials improve the welfare of farmed mink?
Authors: Hansen SW , Malmkvist J , Palme R , Damgaard BM 
Published: January 2023

AbstractThe effects of cage enrichments and additional space were studied in 60 pairs of mink kits kept in standard cages (STD) and 67 pairs of mink kits kept in enriched cages (ENR). During the period from mid July to the end of September both groups had alternate access to one and two connected cages. From October, half of the mink in each group had permanent access to one cage and the other half permanent access to two cages. The enrichment of the cages consisted of extra resting places (tubes made of wire mesh and plastic) and occupational materials in terms of table-tennis balls and ropes to pull and chew. The mink were observed for an experimental period of nine months, from late lactation until the beginning of the following mating season. The welfare was assessed through behavioural traits (use of nest box and enrichments, activity out in the cage, stereotypies and fur-chewing) consumption of food and straw, bodyweight and level of faecal corticoid metabolites. The presence of enrichments resulted in less tail-chewing, fewer stereotypies, and a reduced level of faecal corticoid metabolites. In addition, the presence of enrichments led to fewer social interactions and reduced the consumption of straw. Regarding the frequency of utilising different occupational materials, the mink did not use the table-tennis balls, but the tubes and pull-ropes were given extensive use. Access to one or double cages had no effect on stereotypies, fur-chewing and physiology linked to welfare, but mink with access to double cages used the nest box less, had a lower consumption of straw and pull-ropes than the mink with access to only one cage. However, there were no indications of frustration when the mink were deprived of using double cages. We conclude that increased environmental complexity in the form of occupational materials improved the welfare of the mink, whereas doubling the cage size had little or no effect in relation to mink welfare.
Behaviour of golden hamsters (Mesocricetus auratus) kept in four different cage sizes
Authors: Fischer K , Gebhardt-Henrich SG , Steiger A 
Published: January 2023

AbstractCages for laboratory and pet hamsters are usually small. Using video recordings, the behaviour of sixty female golden hamsters (Mesocricetus auratus), housed individually in four different cage sizes, was compared in order to draw conclusions about their welfare. The cage sizes were 1,800 cm2, 2,500 cm2, 5,000 cm2, and 10,000 cm2. Enrichment items and litter depth were standardised and all cages were equipped with a running-wheel (30 cm diameter). Stereotypic wire-gnawing, usage of the provided space, weight gain, and reactions to mild husbandry stressors were used as welfare indicators. Stereotypic wire-gnawing was observed in all cage sizes, but hamsters in small cages gnawed significantly longer and more frequently. There were no significant differences in running-wheel activity. In small cages hamsters made use of the roof of their wooden shelters as an additional platform more often than in big cages, which could suggest that they needed more space. Therefore, the welfare of pet golden hamsters in cages with a minimal ground floor area of 10,000 cm2seemed to be enhanced compared with smaller cages.
Normal behaviour as a basis for animal welfare assessment
Authors: Wechsler B 
Published: January 2023

AbstractIt is generally agreed that farm animal welfare is at a high level when the animals can behave naturally. Most of today's housing systems, however, differ considerably from the natural environment in which the behavioural organisation of the ancestors of our farm animal species evolved. Consequently, normal behaviour may be impaired in several ways. Frequency, duration or sequence of behavioural elements may be affected. Some normal behaviour patterns may not occur at all. The animals may also possibly behave in unnatural ways – in patterns that would never occur in nature. Furthermore, it is usual for farm animals to exhibit behaviour which is normal in form, but which is elicited by artificial structures within their housing system.In view of these possible changes in normal behaviour, it is necessary to assess, for each farm animal species and each housing system, whether animal welfare is at risk in any way if the behaviour observed differs from the behaviour that would occur in a natural environment. In some cases the question can be answered by taking a theoretical evolutionary approach. In most cases, however, detailed knowledge about the behavioural organisation of the animals is necessary. Such knowledge is built up from animal motivation studies and investigations into the effect of environmental structures on animal behaviour.A specific problem of on-farm animal welfare assessment is that there is often not enough time to collect sufficient data to make a judgement about the occurrence of normal behaviour. Resource-based assessment methods are appropriate as an alternative, provided that the resource standards used are based on evidence stemming from research into animal behaviour and motivation.
The journey to animal welfare improvement
Authors: Whay HR 
Published: January 2023

AbstractThe process of driving improvement in animal welfare has three stages: 1) assessment of animal welfare, 2) identification of the risk factors potentially leading to a compromise in animal welfare, and 3) interventions, in response to the risk factors, to bring about improvement in animal welfare. This process is applicable to animals farmed in commercial situations and for individual animals kept or worked in isolated environments. An impressive weight of knowledge has accumulated in the science of animal welfare assessment and this needs to be counterbalanced by development of mechanisms to actively improve welfare. In the case of animal welfare, interventions have to motivate the animal owner or carer to make changes to their own behaviour on behalf of a third party; the animal. This is a different situation from that described in the human health literature where interventions encourage people to take steps to improve their own well-being, thus benefiting themselves directly. The development of strategies to improve animal welfare require a multi-disciplinary approach including social scientists, psychologists and economists, however, the skills of animal welfare scientists are essential to ensure that interventions truly achieve improvements in animal welfare.
Human and animal behaviour in dairy buffalo at milking
Authors: Saltalamacchia F , Tripaldi C , Castellano A , Napolitano F , Musto M , De Rosa G 
Published: January 2023

AbstractThis study aims to investigate the relationship between human behaviour, buffalo behaviour and prevalence of oxytocin injection at milking. The research was carried out on 17 buffalo farms. On each farm the stockperson's behaviour toward the buffaloes and the buffaloes' behaviour were observed during one afternoon's milking. Stockperson's behaviour was observed from collection of the animals in the waiting area to exit from the milking parlour. The following variables were recorded: number of positive, neutral and negative interactions. The number of steps and kicks were recorded from the entrance to the milking parlour to the removal of cups whenever the stockperson was less than 0.5 m from the animal. The prevalence of oxytocin injection at milking was recorded on two separate occasions with a 5-month interval to obtain a measure of the long-term consistency of this variable. Significant correlations were found between stepping and kicking, stepping and prevalence of oxytocin injection, kicking and prevalence of oxytocin injection and stockperson positive interactions and kicking. These results indicate that stockperson behaviour is related to buffalo behaviour at milking and the latter to the use of oxytocin injections. In addition, prevalence of oxytocin injection proved to be highly reliable when re-tested five months later.
Development of on-farm methods to assess the animal-human relationship in laying hens kept in non-cage systems
Authors: Raubek J , Niebuhr K , Waiblinger S 
Published: January 2023

AbstractThe aim of this study was to develop a set of tests to assess the reaction of laying hens towards humans in non-cage systems and to investigate the repeatability within and between days.Based on a literature survey six tests were developed or modified, which were examined for practicability and adapted in a first phase. Reactions of hens were recorded directly or with a video camera. These included the reaction towards a stationary person: stockperson (SPs); experimenter: (SPe); a moving person: (moving through flock [MP]; approaching individual hens measuring avoidance distance [AD]) and a complex test including stationary and moving elements: (arm moving to touch hens [touch test] in the litter [Tl] and on the slatted area [Ts]). Six flocks of five farms were visited twice, one week apart. At each visit the six tests were performed during morning and afternoon hours. The order of the tests differed between farms.Repeatability of flock reactions within as well as between days was high for SPs, AD and Tl. SPe and especially MP showed slightly reduced correlations. Touching hens at the droppings pit (Ts) was less repeatable than in the litter area (Tl).To sum up, first experiences show that most tests can be practically used in groups of hens in non-cage systems and seem to have good repeatability.
Repeatability of a skin tent test for dehydration in working horses and donkeys
Authors: Pritchard JC , Barr ARS , Whay HR 
Published: January 2023

AbstractDehydration is a serious welfare issue for equines working in developing countries. Risk factors such as high ambient temperature, heavy workload and poor water availability are exacerbated by the traditional belief that provision of water to working animals will reduce their strength or cause colic and muscle cramps.As part of the welfare assessment of 4889 working horses and donkeys during 2002/3, eight observers were trained to perform a standardised skin tent test. The prevalence of a prolonged duration of skin tenting was 50% in horses and 37% in donkeys. Two studies investigated inter-observer repeatability of skin tent test techniques, using a total of 220 horses and donkeys in India and then Egypt: measures of agreement with a ‘gold standard’ observer varied from 40 to 99%. Simplifying the test by reducing the number of possible scores for skin tent from three (immediate return of skin to normal position; delayed return up to three seconds; delayed return more than three seconds) to two (immediate return of skin to normal position; delayed return of any duration) did not improve overall repeatability of the skin tent test. Potential reasons for not achieving high levels of agreement include variations in assessment method, assessors' previous experience, subjective demarcation between score categories and biological variability.
Welfare assessment in extensive animal production systems: challenges and opportunities
Authors: Turner SP , Dwyer CM 
Published: January 2023

AbstractThe welfare of animals in extensive systems has received little attention despite significant welfare challenges in these environments. Additionally, recent reform of Common Agricultural Policy payments in the EU have put increasing financial emphasis on farmers to attain improved levels of animal welfare, although suitable methodologies for assessing animal welfare are lacking. Assessment of welfare in intensive systems frequently involves assessing compliance with buildings and space requirements, as well as behavioural observations of animals in their home pens and monitoring of health records. In extensive systems, however, many of these measures are inappropriate or impossible. Environments are often heterogeneous, animals may be difficult to observe, individuals may not be identifiable and health records may be limited or completely lacking. The expression of fear by extensive animals, which rarely come into close contact with humans, often limits the value and practicality of behavioural observations as welfare indicators. Currently, welfare audits of extensive farms rely greatly on mortality and morbidity records to determine welfare status. Whilst these measures are important, the strong health monitoring bias does not adequately allow an interpretation of the emotional experiences of extensive animals. We suggest that developing methodologies based on assessing key features of the environment, such as handling facilities, assessment of the skills, knowledge and planning of the stockperson, and assessing the animal at key points in the production cycle when they are gathered may allow a workable methodology for assessing animal welfare to be developed for extensive systems.
Evaluation of a welfare indicator protocol for assessing animal welfare in AMS herds: researcher, production advisor and veterinary practitioner opinion
Authors: Rousing T , Jakobsen IA , Hindhede J , Klaas IC , Bonde M , Sørensen JT 
Published: January 2023

AbstractA welfare indicator protocol integrating a total of 38 measures from 4 information sources: housing system, management, animal behaviour and clinical health has been developed for decision support in Automatic Milking System (AMS) herds. Two expert opinion studies focusing on the relevance of the welfare indicator protocol as basis for on-farm welfare assessment have been carried out; one study focused on the opinion of 21 AMS researchers; the other on that of 14 AMS production advisors and 15 veterinary practitioners. The researchers were asked to score the individual welfare relevance of the 38 measures. Furthermore, both the panels were asked to prioritise the 10 most important measures. The 21 researchers generally appreciated the listed protocol measures as highly relevant. The researcher and advisor panels agreed on prioritised measures from all 4 information sources among the 10 most important welfare indicators. In summary, researchers as well as production advisors and veterinarians supported the suggested measures for inclusion in a welfare assessment system. This has demonstrated that integration of different information is considered decisive for operational welfare assessment at herd level.
On-farm welfare assessment systems: what are the recording costs?
Authors: Sørensen JT , Rousing T , Møller SH , Bonde M , Hegelund L 
Published: January 2023

AbstractTo illustrate that different approaches lead to different costs a cost calculation on four different welfare assessment systems for four different animal species has been carried out; an integrated pig herd (450 sows), a dairy cattle herd with automatic milking (90 cows), an organic egg production system (3000 layers) and a mink farm (1000 mink). We calculated the cost to be: €375 per annum for the mink farm and €2205, €2430 and €2435 for the egg production system, the AMS dairy herd and the integrated pig farm, respectively. The costs can be reduced by: reducing the number of indicators and/or the recording frequency, reducing sample sizes, more intensive use of existing data and by exchanging external for internal recordings.