bluuebunny/crossref_metadata_2025_split huggingface dataset:
Gestational diabetes knowledge improves with interactive online training modules: a pre-post analysis
Authors: Krutilova Petra , Williams Roxann , Morey Rebecca , Field Carole , Byrth Veronda , Tepe Melissa , McQueen Amy , Herrick Cynthia 
Published: June 2023

Abstract

Background
The risk of developing type 2 diabetes mellitus (T2DM) is up to 50% among women with gestational diabetes mellitus (GDM). GDM also increases risks for pre-term birth, macrosomia, fetal hypoglycemia, and C-section delivery. Education for expectant mothers with GDM about nutrition, exercise, and the risks of developing T2DM after delivery enhances the probability of postpartum diabetes screening. However, the availability of diabetes education is limited. To bridge this gap, our team developed four training modules on GDM tailored for nurses and community health workers. This pilot study assesses changes in knowledge, self-efficacy for providing diabetes education, attitudes, and intentions to recommend diabetes prevention before and after training completion.
Methods
These interactive online modules, each lasting 45–60 minutes and featuring engaging case studies and integrated knowledge assessment questions, were disseminated through various professional organizations to clinical staff providing care for women with GDM. Optional pre- and post-training surveys were conducted to gauge the effectiveness of the modules. Collected data did not follow a normal distribution pattern. We provided an overview of the baseline characteristics of the population, self-efficacy, attitudes, intentions, and GDM knowledge by calculating the median scores and interquartile ranges. We assessed the changes in scores on self-efficacy, attitudes, intentions, and GDM knowledge before and after training using non-parametric Wilcoxon matched-pair signed rank tests.
Results
Eighty-two individuals completed baseline evaluation and 20 individuals accessed all modules and completed post-training assessments. Among those completing the training, improvement was noted in GDM knowledge [56.5% (16.0) v. 78.3% (22.0), p < 0.001], Self-efficacy for providing diabetes education [6.60 (2.73) v. 9.33 (0.87), p < 0.001], attitudes toward the value of tight control [4.07 (0.79) v. 4.43 (0.86), p = 0.003], and intentions to recommend diabetes prevention measures [4.81 (0.63) v. 5.00 (0.00), p = 0.009)].
Conclusions
Completion of our interactive online modules improved knowledge, intention to recommend diabetes prevention methods, self-efficacy to provide diabetes education, and attitudes toward the value of tight control among individuals caring for women with GDM. Enhanced accessibility to such curricula is crucial to improve access to diabetes education.
Trial registration
This study was registered at clinicaltrials.gov, identifier: NCT04474795.
Green Gospel Effect of Local Financial Expansion: Evidence from Urban Commercial Banks in China
Authors: Song Kaiyi , Bian Yuanchao 
Published: June 2023

Abstract

China's local financial expansion, represented by the development of urban commercial banks, is an important factor affecting the environmental behavior of enterprises, but previous studies have paid less attention to this. We found that both the marginal expansion and the scale expansion of local finance in China help to reduce the sulfur dioxide emission intensity and improve the environmental performance of enterprises, which indicates that there is a green gospel effect of local financial expansion in China. In terms of the impact path, local financial expansion can not only reduce the sulfur dioxide generation intensity, improve the front environmental performance of enterprises; but also increase the sulfur dioxide removal intensity and improve the terminal environmental performance. However, in the context of high fiscal pressure on local governments, local financial expansion exacerbates sulfur dioxide emission and generation intensity of enterprises, worsening environmental performance and creating a green curse effect. Further study finds that the cross-regional expansion of urban commercial banks can strengthen the green gospel effect; the improvement of enterprises’ environmental performance by local financial expansion is mainly found in polluting industries and non-SOEs.
Chromatin Structural Characteristics as an Evidence of Nonhomologous End Joining Repair Pathway Promoting TMPRSS2-ERG Gene Fusion in Prostatic Cancer
Authors: Xu Linfeng , Pan Shen , Huang Haifeng , Fu Yao , Zhang Qing , Zhuang Junlong , Qiu Xuefeng , Gan Weidong , Guo Hongqian 
Published: June 2023

Abstract
Background
TMPRSS2-ERG fusion is the most prevalent gene rearrangement found in prostate cancer and has been linked to prostate carcinogenesis. To investigate the formation mechanism of TMPRSS2-ERG fusion, we performed gene sequencing on ERG fusion prostate cancer and compared them with the normal gene sequence of the Hg19 human genome in the UCSC database.
Methods
We utilized online tools such as QGRS Mapper, QmRLFS-finder, and Palindrome analyzer to predict the possible secondary structures near the fusion point within a range of ± 200bp. We searched for micro-homologous structures in the vicinity of the TMPRSS2-ERG gene fusion point and compared fusion genes with normal genes to identify any base deletions, template insertions, or gene scars formed during the fusion of TMPRSS2 and ERG.
Results
In all cases, the TMPRSS2-ERG gene breakpoints were primarily flanked by cruciform DNA structures, and in some cases, by G-quadruplexes. There were no microhomology structures lost and no base pairs increased during the repair. Blunt junction at the breakpoint was the main linkage approach of TMPRSS2-ERG fusion.
Conclusions
Cruciform DNA structures and G-quadruplexes were the main reason for the failure of homologous recombination repair after TMPRSS2 and ERG gene breakage. The microhomology-mediated end-joining repair was also ruled out due to no microhomology structure loss. Therefore, it can be inferred that TMPRSS2 and ERG breaks are repaired by Nonhomologous End Joining rather than Micro-homologous Mediated End Joining.
Density and Public Transit – Victims of COVID-19 Pandemic?
Authors: Grover Himanshu 
Published: June 2023

AbstractIn recent decades, urban development policies in the Pacific Northwest and elsewhere in the US have distinctly shifted toward promoting sustainable development with higher densities and greater access to public transit. However, these characteristics of the built environment promote COVID-19 transmission owing to greater contact between infected and uninfected individuals. There is growing concern that fear of virus transmission may result in decreased public support for sustainable development. This study analyzes data from a survey conducted in April–May 2022 in Seattle region. The findings suggest that concern about COVID-19 transmission is negatively related to public policy support for higher densityand greater access to public transit. These results are troubling for sustainability advocates because decreased public support may undermine the sustainability agenda. A shift from denser urban areas to low-density suburban communities and from public to individual transportation will result in unsustainable development with long-term negative environmental consequences.
Monoclonal Antibodies in Idiopathic Chronic Eosinophilic Pneumonia: A Scoping Review
Authors: Murillo Andrea Dionelly , Castrillon Ana Isabel , Serrano Carlos Daniel , Fernandez-Trujillo Liliana 
Published: June 2023

Abstract

Background
Idiopathic chronic eosinophilic pneumonia (ICEP) is a rare disease characterized by pulmonary radiological alterations, peripheral eosinophilia, and demonstrated pulmonary eosinophilia. Oral steroids (OSs) are the standard management, but relapses occur in up to 50% of patients during the decrease or suspension of steroids, usually requiring reinitiation of treatment, exposing patients to secondary events derived from the management. Management with monoclonal antibodies has been proposed in these cases to control the disease and limit the secondary effects. The objective is to describe the extent and type of evidence regarding the use of monoclonal antibodies for ICEP.
Methods
A panoramic review of the literature was performed. Observational and experimental studies of pediatric and adult populations that managed recurrent ICEP with monoclonal antibodies were included. Data search, selection, and extraction were performed by two independent reviewers.
Results
937 studies were found. After applying the inclusion and exclusion criteria, 37 titles remained for the final analysis: a retrospective, observational, real-life study, two case series publications, and 34 case reports published in academic poster sessions and letters to the editor. In general, the use of monoclonal antibodies approved for severe asthma could be useful for the control of ICEP, since most of the results show a good response for clinical and radiological outcomes. Biological drugs seem to be a safer option for controlling relapses in ICEP, allowing lowering/suspension of OSs, and sometimes replacing them in patients intolerant to them, patients with significant comorbidities, and patients who have already developed adverse events.
Conclusion
The extent of the evidence supporting management of ICEP with monoclonal antibodies against IL-5 and IgE (omalizumab) is limited, but it could be promising in patients who present frequent relapses, in cortico-dependent individuals, or in patients in whom the use of steroids is contraindicated. The extent of the evidence for management with dupilumab is more limited. Studies with better design and structure are needed to evaluate quality of life and outcomes during a clear follow-up period. To our knowledge, this is the first scoping review of the literature showing the extent of the evidence for the management of ICEP with monoclonal antibodies.
Blastic Plasmacytoid Dendritic Cell Neoplasm: Challenges in Diagnosis and Treatment with Potential of Venetoclax as an Alternative to Vincristine in High-Risk Patients-A case report
Authors: Syed Naveed , Jaber Waed Mohammad , Elkonaissi Islam Samir , Mirza Imran , Damlaj Moussab 
Published: June 2023

Abstract
Background: Blastic plasmacytoid dendritic cell neoplasm (BPDCN) is a rare and challenging cancer for diagnosis and treatment. Accurate diagnosis plays a crucial role guiding appropriate treatment, typically involving high-intensity lymphoblastic leukemia regimens which typically include vincristine. However, the use of vincristine may be particularly limited in patients with pre-existing neuropathy or individuals at high risk of developing it. Here, we present a case of BPDCN that was initially diagnosed as Marginal Zone Lymphoma (MZL) and subsequently as non-specific T-cell lymphoma, thus highlights the importance of accurate diagnosis and modified treatment.
Case presentation: A 49-year-old Arab man with a medical history of diabetes, peripheral neuropathy, hypertension, and depression presented with widespread, painless multiple skin lesions. After undergoing a biopsy at another institution, the patient was initially diagnosed with MZL, and received two cycles of bendamustine and rituximab. However, the disease relapsed and was later diagnosed with non-specific T-cell lymphoma, which proved refractory to a single cycle of CHOP chemotherapy. The patient was subsequently referred to our centre, where a comprehensive evaluation revealed BPDCN with a unique finding on bone marrow exam: signet ring plasmacytoid dendritic cells. Due to the patient's pre-existing neuropathy and previous treatment, we administered the Hyper-CVAD regimen with a 50% reduction in vincristine dosage, which resulted in an excellent response. During the second part of cycle one, when new skin lesions started appearing, venetoclax was added to the treatment regimen. Subsequently, vincristine was completely removed, and venetoclax was continued in cycle two, leading to a complete response. The patient achieved a disease-free state for the first time in disease course, maintaining it for a period of over six weeks before experiencing a relapse.
Conclusion: Accurate diagnosis is crucial for guiding appropriate treatment. Our case highlights the challenges associated with diagnosis and treatment, as well as the potential of venetoclax as an alternative to vincristine, particularly in patients with pre-existing neuropathy or those at a high risk of developing it. Further research is needed to evaluate the effectiveness of BCL2 inhibitors as a replacement for essential drugs and its potential as a bridging therapy until patients can undergo a stem cell transplant.Top of Form
FKBP5 activates mitophagy by ablating PPAR-γ to shape a benign remyelination environment
Authors: Sun Xingzong , Qian Menghan , Li Hongliang , Wang Lei , Zhao Yunjie , Yin Min , Dai Lili , Bao Hongkun 
Published: June 2023

Abstract
Multiple sclerosis (MS) is an autoimmune and neurodegenerative disease of the central nervous system (CNS) that is characterized by myelin damage followed by axonal and ultimately neuronal loss, which has been found to be associated with mitophagy. The etiology and physiopathology of MS remain elusive. However, the role of FK506 binding protein 5 (FKBP5, also called FKBP51), a newly identified gene associated with MS, in the progression of the disease has not been well defined. Here, we observed that the progress of myelin loss and regeneration in Fkbp5ko mice treated with demyelination for the same amount of time was significantly slower than in wild-type mice and that mitophagy plays an important regulatory role in this process. To investigate the mechanism, we discovered that the levels of FKBP5 protein were greatly enhanced in the CNS of cuprizone (CPZ) mice and found that the myelin-denuded environment stimulates significant activation of the PINK1-Parkin pathway of mitophagy, in which the important regulator, PPAR-γ, is critically regulated by FKBP5. This study reveals the role of FKBP5 in regulating a dynamic pathway of natural restorative regulation of mitophagy through PPAR-γ in pathological demyelinating settings, which may provide potential targets for the treatment of demyelinating diseases.
IgM N-glycosylation correlates with COVID-19 severity and rate of complement deposition
Authors: Haslund-Gourley Benjamin , Woloszcuk Kyra , Hou Jintong , Connors Jennifer , Cusimano Gina , Bell Mathew , Taramangalam Bhavani , Fourati Slim , Mege Nathan , Bernui Mariana , Altman Matthew , Krammer Florian , Bakel Harm van , Maecker Holden , Wigdahl Brian , Cairns Charles , Haddad Elias , Comunale Mary 
Published: June 2023

Abstract
The glycosylation of IgG plays a critical role during human SARS-CoV-2, activating immune cells and inducing cytokine production. However, the role of IgM N-glycosylation has not been studied during acute viral infection in humans. In vitro evidence suggests that the glycosylation of IgM inhibits T cell proliferation and alters complement activation rates. The analysis of IgM N-glycosylation from healthy controls and hospitalized COVID-19 patients reveals that mannosylation and sialyation levels associate with COVID-19 severity. Specifically, we find increased di- and tri-sialylated glycans and altered mannose glycans in total serum IgM in severe COVID-19 patients when compared to moderate COVID-19 patients. This is in direct contrast with the decrease of sialic acid found on the serum IgG from the same cohorts. Moreover, the degree of mannosylation and sialylation correlated significantly with markers of disease severity: D-dimer, BUN, creatinine, potassium, and early anti-COVID-19 amounts of IgG, IgA, and IgM. Further, IL-16 and IL-18 cytokines showed similar trends with the amount of mannose and sialic acid present on IgM, implicating these cytokines' potential to impact glycosyltransferase expression during IgM production. When examining PBMC mRNA transcripts, we observe a decrease in the expression of Golgi mannosidases that correlates with the overall reduction in mannose processing we detect in the IgM N-glycosylation profile. Importantly, we found that IgM contains alpha-2,3 linked sialic acids in addition to the previously reported alpha-2,6 linkage. We also report that antigen-specific IgM antibody-dependent complement deposition is elevated in severe COVID-19 patients. Taken together, this work links the immunoglobulin M N-glycosylation with COVID-19 severity and highlights the need to understand the connection between IgM glycosylation and downstream immune function during human disease.
Loss of ELAVL2 is associated with aggressive mesenchymal transition in glioblastoma
Authors: Kim Yona , You Ji Hyeon , Ryu Yeonjoo , Park Gyuri , Lee Urim , Moon Hyo Eun , Park Hye Ran , Song Chang , Ku Ja-Lok , Park Sung-Hye , Paek Sun Ha 
Published: June 2023

Abstract
Glioblastoma (GBM), the most lethal type of primary brain cancer, is characterized by cellular and molecular plasticity, which leads to intratumoral heterogeneity and hinders effective treatment. However, the regulation of such plasticity, including mesenchymal (MES) transition, is poorly understood. Here, we demonstrate that the RNA-binding protein ELAVL2 regulates aggressive MES transformation in GBM. ELAVL2 was most frequently deleted in GBM compared to other cancers and associated with distinct clinical and molecular features. ELAVL2-mediated transcriptomic alterations were indicative of GBM subtype signatures. Expression of ELAVL2 negatively correlated with that of epithelial-to-mesenchymal transition (EMT)-related genes, and its loss promoted the EMT process and chemo-resistance. Tissue microarray analysis revealed that high ELAVL2 protein expression level confers a favorable survival in GBM patients. On a molecular level, ELAVL2 regulated the expression of EMT-inhibitory molecules SH3GL3 and DNM3. Overall, these findings demonstrate ELAVL2 as a critical tumor suppressor that regulates MES transition in GBM, highlighting its role in transcriptomic plasticity and glioma progression.
Liposomal irinotecan plus fluorouracil/leucovorin in older patients with advanced pancreatic cancer: a single-center retrospective study
Authors: Nagashima Shuhei , Kobayashi Satoshi , Tsunoda Shotaro , Yamachika Yui , Tozuka Yuichiro , Fukushima Taito , Morimoto Manabu , Ueno Makoto , Furuse Junji , Maeda Shin 
Published: June 2023

Abstract
Background: The global phase 3 NAPOLI -1 trial of patients with pancreatic ductal adenocarcinoma demonstrated an overall survival (OS) benefit from using liposomal irinotecan and 5-fluorouracil/leucovorin (nal-IRI+5-FU/LV) after treatment with gemcitabine when compared to 5-FU/LV alone. However, the efficacy and safety of this regimen in older patients are not well studied.
Methods: This single-center retrospective study compared results of nal-IRI+5FU/LV between older and younger patients.
Results: Of 115 patients, 54 (47.0%) and 24 (20.9%) were aged ≥70 and ≥75 years, respectively. The median OS and progression-free survival of the entire cohort were 8.5 and 3.6 months, respectively. There were no significant differences in OS and PFS hazard ratios using cutoffs of 70 years (P = 0.90 and 0.99, respectively) and 75 years (P = 0.90 and 0.76, respectively). There were also no significant differences in the incidence of treatment-related adverse events between patients aged ≥70 and <70 years, or those aged ≥75 and <75 years. Other than hematological toxicity, no treatment-related adverse events higher than Grade 4 were observed in either age group.
Conclusion: The efficacy and safety of nal-IRI+5-FU/LV for patients with pancreatic ductal adenocarcinoma are not significantly different for patients aged 70 years and older compared to younger patients.
The chicken cGAS-STING pathway exerts interferon independent antiviral function via cell apoptosis
Authors: Jiang Sen , Lv Mengjia , Zhang Desheng , Cao Qi , Xia Nengwen , Luo Jia , Zheng Wanglong , Chen Nanhua , Meurens François , Zhu Jianzhong 
Published: June 2023

Abstract
It has been recently recognized that the DNA sensing innate immune cGAS-STING pathway exerts an IFN-independent antiviral function; however, whether and how chicken STING (chSTING) exerts such an IFN-independent antiviral activity is still unknown. Here, we showed that chSTING exerts an antiviral activity in both mammalian cells and chicken cells, either with or without the production of IFN. On the other hand, chSTING was able to trigger cell apoptosis and autophagy independently of IFN, and the apoptosis inhibitors instead of autophagy inhibitors could antagonize the antiviral function of chSTING, suggesting the involvement of apoptosis in the IFN independent antiviral function. In addition, chSTING lost its antiviral function in IRF7 knockout chicken macrophages, indicating that IRF7 is not only essential for the production of IFN, but also participates in the other activities of chSTING, such as the apoptosis. Collectively, our results showed that chSTING exerts the antiviral function independently of IFN, likely via the apoptosis.
Accuracy assessment and uncertainty of the 10 meter resolution land use land cover maps at local scale. Case: talassemtane national park, Morocco
Authors: Chemchaoui Abdelazziz , Brhadda Najiba , Alaoui Hassana Ismaili , Souad El Gazi , Bouchra El Asri , Rabea Ziri 
Published: June 2023

Abstract
Global Land use land cover (LULC) maps play an important role in monitoring forest 1 dynamics and are a tool for any spatial planner. More and more institutes are interested in the 2 production of such maps, especially with high-resolution imagery freely available and the emergence 3 of new techniques. But they should be submitted to an accuracy assessment for accurate and better 4 use at the local scale. The two products that are the subject of this study are ESA Worldcover 2020 5 and ESRI Landcover 2020. These two products have been subjected to an accuracy assessment in 6 Talassemtane National Park(TNP). The results showed an overall accuracy of 58.06% for the ESA 7 product and 78.97% for the ESRI product. In addition, the Area-based accuracy was conducted to 8 adjust each class’s area and evaluate uncertainty. Finally, the spatial agreement between the two 9 products showed a result of 66,43 % at the local level in the TNP. This work will be used for research 10 studies in the Talassemtane National Park area, particularly for evaluating ecosystem services.
The patient-specific mouse model with Foxg1 frameshift mutation uncovers the pathophysiology of FOXG1 syndrome
Authors: Park Jaein , Moon Ji Hwan , O’Shea Holly , Shin Dongjun , Hwang Seon Ung , Li Liwen , Lee Hyojong , Brimble Elise , Lee Jae , Clark Stewart , Lee Soo-Kyung , Jeon Shin 
Published: June 2023

Abstract
Single allelic mutations in the gene encoding the forebrain-specific transcription factor FOXG1 lead to FOXG1 syndrome (FS). Patient-specific animal models are needed to understand the etiology of FS, as FS patients show a wide spectrum of symptoms correlated with location and mutation type in the FOXG1 gene. Here we report the first patient-specific FS mouse model, Q84Pfs heterozygous (Q84Pfs-Het) mice, mimicking one of the most predominant single nucleotide variants in FS. Intriguingly, we found that Q84Pfs-Het mice faithfully recapitulate human FS phenotypes at the cellular, brain structural, and behavioral levels. Importantly, Q84Pfs-Het mice exhibited myelination deficits like FS patients. Further, our transcriptome analysis of Q84Pfs-Het cortex revealed a new role for FOXG1 in synapse and oligodendrocyte development. The dysregulated genes in Q84Pfs-Het brains also predicted motor dysfunction and autism-like phenotypes. Correspondingly, Q84Pfs-Het mice showed movement deficits, repetitive behaviors, increased anxiety, and prolonged behavior arrest. Together, our study revealed the crucial postnatal role of FOXG1 in neuronal maturation and myelination and elucidated the essential pathophysiology mechanisms of FS.