bluuebunny/crossref_metadata_2025_split huggingface dataset:
Short-term intermittent hypoxia therapy promotes gliogenesis in a rat model of middle cerebral artery occlusion (MCAO) stroke
Authors: Roshan Syed Aasish , Gunaseelan Dharani , Jayachandran Swaminathan K , Kandasamy Mahesh , Anusuyadevi Muthuswamy 
Published: June 2023

AbstractAimAfter focal cerebral ischemia, intermittent hypoxia therapy (IHT) could be used as a non-invasive method to stabilize and stimulate neurogenesis in the innate stem cell niche in the brain, and disrupt the glial scar around the infarct to increase neuroblast migration in the striatal infarct area.MethodsWe induced focal cerebral ischemia in Wistar albino rats using the MCAo model. A week later, animals were subjected to intermittent hypoxia (12%O2, 4hr/day) for a period of 14 days. Post-treatment analysis of functional recovery and cellular regeneration was done using immunofluorescence analysis of multiple neuronal cell markers including Doublecortin (DCX), Nestin, and Vimentin among others.ResultsObservations of GFAP-positive cells revealed that IH treatment facilitates gliogenesis in the infarct striatal region of a rat model of MCAo stroke. The percentage of DCX and GFAP double-positive cells was increased in the IH-treated group. Also, there was a significant difference in the morphology of vimentin-positive cells and microglia cells between the stroke groups.ConclusionThese outcomes suggest that exposure of MCAo stroke-affected rats to intermittent hypoxia results in an increase in migrated neuroblasts resulting in a subsequent altered glial scar integrity in the infarct region, thus suggesting an alternative non-invasive method against the common stem cell transplant techniques, to increase endogenous neuroblasts in the infarct area after stroke.
A whole-organism landscape of X-inactivation in humans
Authors: Gylemo Björn , Bensberg Maike , Nestor Colm E. 
Published: June 2023

AbstractAs females are mosaic for X-inactivation, direct determination of X-linked allelic expression in bulk tissues is typically unfeasible. Using females that are non-mosaic for X-inactivation (nmXCI) has proven a powerful and natural genetic system for profiling X-inactivation in humans. By combining allele-resolution data for one previously reported and two newly identified nmXCI females, we directly determined X-inactivation status of 380 X-linked genes across 30 normal tissues, including 198 genes for which XCI status is directly determined for the first time. Our findings represent a substantial advance in our understanding of human X-inactivation and will serve as a reference for dissecting the genetic origin of sex-bias in human traits. In addition, our study reveals nmXCI as a common feature of the human female population, with profound consequences for the penetrance and expressivity of X-linked traits in humans.
Interaction molecular QTL mapping discovers cellular and environmental modifiers of genetic regulatory effects
Authors: Kasela Silva , Aguet François , Kim-Hellmuth Sarah , Brown Brielin C. , Nachun Daniel C. , Tracy Russell P. , Durda Peter , Liu Yongmei , Taylor Kent D. , Johnson W. Craig , Van Den Berg David , Gabriel Stacey , Gupta Namrata , Smith Joshua D. , Blackwell Thomas W. , Rotter Jerome I. , Ardlie Kristin G. , Manichaikul Ani , Rich Stephen S. , Barr R. Graham , Lappalainen Tuuli 
Published: June 2023

AbstractBulk tissue molecular quantitative trait loci (QTLs) have been the starting point for interpreting disease-associated variants, while context-specific QTLs show particular relevance for disease. Here, we present the results of mapping interaction QTLs (iQTLs) for cell type, age, and other phenotypic variables in multi-omic, longitudinal data from blood of individuals of diverse ancestries. By modeling the interaction between genotype and estimated cell type proportions, we demonstrate that cell type iQTLs could be considered as proxies for cell type-specific QTL effects. The interpretation of age iQTLs, however, warrants caution as the moderation effect of age on the genotype and molecular phenotype association may be mediated by changes in cell type composition. Finally, we show that cell type iQTLs contribute to cell type-specific enrichment of diseases that, in combination with additional functional data, may guide future functional studies. Overall, this study highlights iQTLs to gain insights into the context-specificity of regulatory effects.
G2C4targeting antisense oligonucleotides potently mitigate TDP-43 dysfunction in C9orf72 ALS/FTD human neurons
Authors: Rothstein Jeffrey D. , Baskerville Victoria , Rapuri Sampath , Mehlhop Emma , Jafar-nejad Paymaan , Rigo Frank , Bennett Frank , Mizielinska Sarah , Isaacs Adrian , Coyne Alyssa N. 
Published: June 2023

AbstractThe G4C2repeat expansion in the C9orf72 gene is the most common genetic cause of Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Many studies suggest that dipeptide repeat proteins produced from this repeat are toxic, yet, the contribution of repeat RNA toxicity is under investigated and even less is known regarding the pathogenicity of antisense repeat RNA. Recently, two clinical trials targeting G4C2(sense) repeat RNA via antisense oligonucleotide failed despite a robust decrease in sense encoded dipeptide repeat proteins demonstrating target engagement. Here, in this brief report, we show that G2C4antisense, but not G4C2sense, repeat RNA is sufficient to induce TDP-43 dysfunction in induced pluripotent stem cell (iPSC) derived neurons (iPSNs). Unexpectedly, only G2C4, but not G4C2sense strand targeting, ASOs mitigate deficits in TDP-43 function in authentic C9orf72 ALS/FTD patient iPSNs. Collectively, our data suggest that the G2C4antisense repeat RNA may be an important therapeutic target and provide insights into a possible explanation for the recent G4C2ASO clinical trial failure.
Cross regional coordination of neural activity in the human brain during autobiographical self-referential processing
Authors: Stieger James R. , Pinheiro-Chagas Pedro , Fang Ying , Lusk Zoe , Perry Claire M. , Wagner Anthony , Contreras Diego , Chen Qi , Hugenard John , Buch Vivek , Parvizi Josef 
Published: June 2023

SUMMARYFor the human mind to operate, populations of neurons across remote regions of the brain need to coordinate their activity in the subsecond temporal scale. To date, our knowledge of such fast interactions involving cortical and subcortical structures in large brains, such as the human brain, remains limited. Here, we used stereo-electroencephalography (sEEG) recordings across four brain regions that are known, from decades of work, to be important for autobiographical memory processing. Our recordings involved 31 human participants implanted with intracranial electrodes in the hippocampus (HPC), posteromedial cortex (PMC), and ventromedial, as well as orbital subregions of the prefrontal cortex (OFC). In 14 subjects, we also recorded simultaneously in the anterior thalamus (ANT) across various experimental conditions and with direct electrical stimulations. Our observations provide new lines of correlative and causal evidence about the spatiotemporal profile of oscillatory coordination of cortical and subcortical activity during self-referential memory-based processing.
Investigating Metabolic Trends in the Oral Cavity to Identify Novel Metabolites
Authors: Okiye Maribel E.K. , Velez Michelle A. , Sugai James , Kinney Janet , Giannobile William V. , Tripathi Ashootosh , Sherman David H 
Published: June 2023

AbstractThe human oral microbiome typically contains over 700 different microbial species. These interactions between the microorganisms within this community can shape the microenvironment throughout the human body, as these interactions are paramount to maintaining oral and overall systemic health. Recent advances in technology, such as next-generation sequencing (NGS), have revealed the complexities of the oral microbiome, linking dysbiosis of the oral microbiome with several chronic ailments such as cardiovascular disease, diabetes, and inflammatory bowel disease. However, the role of microbial secondary metabolites in oral and systemic disease progression remains poorly understood. Here, we conducted a metabolomics study on the human salivary secondary metabolome during the induction of gingival inflammation (gingivitis), the precursor to periodontal disease. In this study, we sought to assess the changes in the oral secondary metabolome during disease progression by emulating dysbiosis of the oral microbiome through a twenty-one-day induction of gingivitis in twenty human participants. We identified secondary metabolites, cyclo(L-Tyr-L-Pro) with regulatory properties for quorum sensing and inflammatory marker secretion, indicating a specialized role for secondary metabolites in oral health maintenance. Surprisingly, we also uncovered a previously unknown metabolic lag that occurs during dysbiosis recovery of the oral cavity, which indicates a lingering presence of signaling molecules for pathogenic microbe proliferation or a total oral metabolome modification following microenvironmental stress in the oral cavity. This work represents a high-resolution metabolomic landscape for understanding oral health during gingivitis that opens new opportunities for combating progressive periodontal diseases and sepsis due to the translocation of oral microbes in the human body.
Spatial correspondence in relative space regulates serial dependence
Authors: Lim Jaeseob , Lee Sang-Hun 
Published: June 2023

AbstractOur perception is often attracted to what we have seen before, a phenomenon called ‘serial dependence.’ Serial dependence can help maintain a stable perception of the world, given the statistical regularity in the environment. If serial dependence serves this presumed utility, it should be pronounced when consecutive elements share the same identity when multiple elements spatially shift across successive views.However, such preferential serial dependence between identity-matching elements in dynamic situations has never been empirically tested. Here, we hypothesized that serial dependence between consecutive elements is modulated more effectively by the spatial correspondence in relative space than by that in absolute space because spatial correspondence in relative coordinates can warrant identity matching invariantly to changes in absolute coordinates. To test this hypothesis, we developed a task where two targets change positions in unison between successive views. We found that serial dependence was substantially modulated by the correspondence in relative coordinates, but not by that in absolute coordinates. Moreover, such selective modulation by the correspondence in relative space was also observed even for the serial dependence defined by previous non-target elements. Our findings are consistent with the view that serial dependence subserves object-based perceptual stabilization over time in dynamic situations.
Innovative instructional approach: the effect of information and communication technology-assisted instruction on civic education students' performance
Authors: Misan-Ruppee Rachael Oke , Obro Sunday , Akpochafo Williams Pius 
Published: June 2023

PurposeThe study explored the effect of information and communication technology-assisted instruction (ICTAI) on civic education (CE) students' academic performance and the effect of gender on students' performance. Specifically, the study addressed the facilitating effect of ICTAI on students' scholarly performance.Design/methodology/approachThis study utilised the quasi-experimental approach. A sample of 255 senior secondary school students was the analysis unit. A teacher-made performance test was used as an instrument for the study. The data were analysed with descriptive statistics for the research questions, and hypotheses tested with paired sample t-test statistics.FindingsThe study results indicated that ICTAI improved student scholarly performance. The study also proved that gender had an impact on student academic performance.Research limitations/implications Not all the students in intact classes that were used for conducting the study/research, and the background and gender of research assistants were not considered, and these could have affected the result of the study. The study implications are that this study is a piece of deeper ingenuity and innovation that provides an additional source of information to study. The study provided proof that innovative activity-based instructions such as computer-assisted instruction (CAI) boost students’ performance.Practical implicationsOn the instructor, the study findings demonstrated the significant effect of ICTAI on the scholarly performance of CE students. Consequently, this study contends that CE instructors/teachers can benefit from the implications of these findings by comprehensively understanding that ICTIA usage enhances student performance and, thus, improves the students' performance. Additionally, the study's findings proved that gender affected students' performance when instructed with ICTAI. The study implies that CE instructors/teachers should pay special attention to students' gender, specifically female students, as gender affects the improvement of students' CE performance when instructed with ICTAI.Originality/valueThe study findings contribute to the literature on academic improvement and performance of CE students by enhancing the understanding of the effect of ICTAI on students' scholarly performance. The study recognises the existing gaps in previous literature and vivid understanding of the effect of ICTAI on students' performance and gender as a mediator.
An overview of the SWOT analysis in India's pharmaceutical supply chain
Authors: Wahab Siti Norida , Ahmed Nusrat , Ab Talib Mohamed Syazwan 
Published: June 2023

PurposeThe Indian pharmaceutical industry has contributed significantly to global healthcare by securing superior-quality, inexpensive and reachable medicines worldwide. However, supply chain management (SCM) has been challenging due to constantly shifting requirements for short lifecycles of products, the convergence of industry and changeable realities on the ground. This study aims to identify, assess and prioritize the strengths, weaknesses and opportunities of the pharmaceutical SCM environment in India.Design/methodology/approachThe paper employs a Strength, Weakness, Opportunity, Threat (SWOT) analysis and recognizes strategies to utilize the advantages of the strengths and opportunities, rectify weaknesses and resolve threats.FindingsA variety of strategies that could have a positive effect on the Indian pharmaceutical business are presented. Findings and suggested strategies can significantly advance knowledge, enhance understanding and contribute to the growth of a successful SCM for the Indian pharmaceutical sector.Originality/valueThis paper would act as a roadmap to greater comprehension of the market leaders and market leaders' operating climate. The findings from this study will offer academic scholars and business practitioners deeper insights into the environment of SCM.
A framework for social sustainability on the building level: a contextual approach
Authors: Sharif Ahlam Ammar 
Published: June 2023

Purpose
This study aims to build on a contextualised approach for revealing the particularities of social sustainability indicators on the building scale within the particular context of Jordan, focusing on the buildings of the Dahiyat Al Hussein suburb.

Design/methodology/approach
This study relied on a review of the relevant previous research as well as global, regional and local rating tools, followed by working with a diversified participant base comprising experts as well as users through Delphi rounds and analytic hierarchy process-based assessments.

Findings
This study resulted in several key indicators that were classified into three main categories: environmental friendliness, comfort and convenience and social blend. It resembled a step on the path towards the standardisation of context-specific social sustainability indicators, an effort that would be further supported by future research addressing other development within Jordan.

Originality/value
The sustainability debate has, for long, acquired the undivided attention of its key stakeholders. With the continuous rise of global attention, the conceptualisation of sustainability has grown more specialised in both function and scale, and sustainable measures have been developed at the macro and micro levels. With the further spread of the concept, recognition of its contextual differences between various countries became more vivid, where the social dimension attains particular importance.
Managing geopolitical risks: the global oil and gas industry plays a winning game
Authors: Gamso Jonas , Inkpen Andrew , Ramaswamy Kannan 
Published: June 2023

Purpose
Geopolitical risks associated with the return of great power politics and growing nationalism have generated new challenges for foreign investors across industries. Oil and gas companies are well acquainted with such risks and have developed strategies to manage them. This paper reviews five of these strategies: divorcing ownership control from operating control in designing collaborative ventures; proactively managing stakeholder relationships; ensuring transparency and communication; diversifying risks while proactively positioning for emerging opportunities; and deliberately planning for exit should such an eventuality arise. Firms outside of oil and gas can draw on these strategies as they navigate the emerging geopolitical context.

Design/methodology/approach
This paper reviews five strategies that oil and gas companies can use to manage geopolitical risk: divorcing ownership control from operating control in designing collaborative ventures; proactively managing stakeholder relationships; ensuring transparency and communication; diversifying risks while proactively positioning for emerging opportunities; and deliberately planning for exit should such an eventuality arise.

Findings
This study identifies several strategies that oil and gas companies have used to manage geopolitical risks. These tools will be increasingly important in the shifting global political landscape.

Originality/value
Drawing on the experiences of oil and gas companies, this study has identified several strategies that companies can use to shield themselves from the risks that are currently emanating from geopolitics. While these best practices originate in the experiences of oil and gas firms, the ability to deftly manage geopolitical risks is becoming an important prerequisite for companies across industries.
Impact of fashion influencers on consumers' purchase intentions: theory of planned behaviour and mediation of attitude
Authors: Tiwari Archana , Kumar Audhesh , Kant Rishi , Jaiswal Deepak 
Published: June 2023

PurposeThe purpose of this study is to examine the impact of fashion influencer measures on consumers' purchase intentions and the mediation of attitudes to understand the phenomenon of influencer marketing in the backdrop of the fashion industry.Design/methodology/approachThe present study employs a conceptual model based on extended theory of planned behaviour (TPB) with added perceived trust. Data were collected from 341 participants from different regions of the country and analysed using direct path analysis and mediation technique.FindingsThe study found that attitudes toward fashion influencers are positively influenced by perceived trust, subjective norms and perceived behavioural control. However, perceived behavioural control is not directly related to purchasing intents in the research model. The results confirmed that attitudes have a positive association with purchase intentions both directly and indirectly (partially mediation).Research limitations/implicationsThe study advocates market practitioners and advertisers to acknowledge the increasing importance of influencer marketing and the promotion of their fashion offerings in the setting of emerging fashion industry.Originality/valueThe present study adds crucial value to enhance the understanding of fashion influencer marketing in the Indian context. This research offers several insights into the continually growing knowledge domain of influencer marketing by predicting the direct relationships with purchase intents and the mediation of attitudes.
Knowledge codifiability, common interests and knowledge transfer: the inhibiting role of system dependence under increasing novelty
Authors: Acharya Chandan , Sigdyal Pratigya , Ojha Divesh , Patel Pankaj C. , Dhir Amandeep 
Published: June 2023

Purpose
This paper aims to address the challenges knowledge actors face when using knowledge codifiability to develop common interests. The challenge is compounded when actors with diverse knowledge domains depend on each other to complete tasks, and, simultaneously, update their knowledge to address novelty in the organizational environment.

Design/methodology/approach
Given this context, this paper studies the impact of two moderating variables, systems dependence (Z) and novelty (W), on the relationship between knowledge codifiability (X) and common interests (M). This study also examines whether common interests (M) mediate the relationship between knowledge codifiability (X) and knowledge transfer (Y). To test the hypotheses, the authors collected data from 163 entrepreneurs in the southwest USA.

Findings
The results demonstrate that novelty in the knowledge domain of actors provides a supporting context for knowledge codifiability to develop common interests, but only when actors’ dependence on each other to complete tasks is at low to medium level. Moreover, the results also provide evidence that common interests mediate the relationship between codifiability and ease of knowledge transfer.

Originality/value
Using the results, this study provides a decision-making framework for managing tasks based on system dependence and novelty level.
Formative research for a social marketing safety campaign at a biomedical research facility
Authors: Lagoe Carolyn A. , Newcomer Derek , Fico Ashley 
Published: June 2023

Purpose
The purpose of this study is to consider the potential use of social marketing to enhance safety compliance in a biomedical research laboratory.

Design/methodology/approach
This project used focus group discussions (n = 32) and semi-structured interviews (n = 10) to gather information on occupational health and safety professionals’ perceptions of safety culture, knowledge of barriers and facilitators of safety at the worksite and insights on how to effectively communicate safety information to employees through a campaign effort.

Findings
Results outlined the barriers (e.g. structural obstacles, lack of awareness of policies, perception of occupational safety and health professionals as safety police) and facilitators (e.g. rapport building, partnering with formal leadership and demonstrating the value of services) of safety and safety communication.

Originality/value
Results from this work add to theoretical and practical knowledge regarding how risk may be effectively addressed by using social marketing and health behavior theories to promote voluntary adherence to existing rules and recommendations.