bluuebunny/crossref_metadata_2025_split huggingface dataset:
Inland navigation is a driver of freshwater biodiversity declines in Europe
Authors: Sexton Aaron , Beisel Jean-Nicolas , Staentzel Cybill , Wolter Christian , Tales Evelyne , Belliard Jérôme , Buijse Anthonie , Fernández Vanesa Martinez , Wantzen Karl , Jähnig Sonja , de Leaniz Carlos Garcia , Schmidt-Kloiber Astrid , Haase Peter , Forio Marie , Archambaud Gait , Fruget Jean-François , Dohet Alain , Evtimova Vesela , Csabai Zoltán , Floury Mathieu , Goethals Peter , Várbíró Gábor , Cañedo-Argüelles Miguel , Larrañaga Aitor , Maire Anthony , Schaefer Ralf , Sinclair James , Vannevel Rudy , Welti Ellen , Jeliazkov Alienor 
Published: September 2023

Abstract

Freshwater navigation is expected to increase in the coming years, being promoted as a low-carbon form of transport. However, we currently lack knowledge on how this will impact biodiversity at large scales and interact with existing stressors. We addressed this knowledge gap by analyzing fish and macroinvertebrate community time series spanning the last 30 years across large European rivers comprising 19,592 observations from 4,049 sampling sites. We found ship traffic to be associated with biodiversity declines, i.e. decreases in fish and macroinvertebrate taxonomic richness and diversity, and trait richness. Shipping was also associated with increases in taxonomic evenness, which, in concert with richness decreases, can likely be attributed to losses in rare/smaller populations. In particular, shipping was especially harmful for benthic taxa and those preferring slow flows. These effects were often dependent on local land use and degradation. In fish, the negative impacts of shipping were highest in urban and agricultural landscapes. Regarding navigation infrastructure, the negative impact of channelization on macroinvertebrates was only evident when riparian degradation was also high. Our results demonstrate the risk of increasing inland navigation on freshwater biodiversity. Integrative waterway management accounting for riparian and landscape characteristics could help to mitigate these impacts.
Cloning and expression analysis of beta-tubulin gene in Tetrahymena pyriformis following infection of the guppy
Authors: Jiang Mingyue , Zhou Chunyu , Wang Sihan , Liu Lihui , Zhang Shuixian , Wang Li , Pan Xuming 
Published: September 2023

Abstract
Tetrahymenosis is caused by the ciliated protozoan Tetrahymena and is responsible for serious economic losses to the aquaculture industry worldwide. However, information regarding the molecular mechanism leading to tetrahymenosis is limited. In previous transcriptome sequencing work, it was found that β-tubulin gene was significantly expressed in T. pyriformis during its infected fish, we speculated that β-tubulin involved in Tetrahymena piriformis infecting fish. Herein, the potential biological function of the β-tubulin gene in Tetrahymena species when establishing infection in guppies was investigated by cloning the full-length cDNA of T. pyriformis β-tubulin gene (TUBB). The full-length cDNA of TUBB was 1873 bp, and the ORF occupied 1134 bp, whereas 5' UTR 434 bp, and 3' UTR 305 bp whose poly (A) tail contained 12 bases. The predicted protein encoded by TUBB had a calculated molecular weight of 42.26 kDa and pI of 4.48. Moreover, secondary structure analysis and tertiary structure prediction of TUBB protein were also conducted. In addition, morphology, infraciliature, phylogeny, and histopathology of T. pyriformis isolated from guppies from a fish market in Harbin were also investigated. Furthermore, qRT-PCR analysis and experimental infection assays indicated that the expression of TUBB resulted in efficient cell proliferation during infection. Collectively, our data revealed that TUBB is a key gene involved in T. pyriformis infection in guppies, and the findings discussed herein provide valuable insights for future studies on tetrahymenosis.
The behavior of phobic and philic oil mist filters under high pressure
Authors: wang yuewen , Wu Xiaolin , Chen Yuncong , Ji Zhongli 
Published: September 2023

Abstract
The aim of this paper is to study the changing law of filter material properties under high pressure conditions, and for this purpose an experimental setup was established to measure the properties of filter materials at a maximum pressure of 5 MPa. The experiments were carried out at a pressure of 1–5 MPa, and the detailed experiments were conducted on the pressure drop, saturation and the liquid distribution pattern of the first and last layers of the filter material. The experimental results show that the liquid film on the surface of the filter material becomes progressively thinner with increasing pressure, and the jump pressure drop becomes smaller while the saturation increases. In addition, the results show that with increasing pressure, the surface tension of the droplets decreases and the surface tension of the DEHS decreases from 28.72 mN/m to 25.26 mN/m. The capillary force of filter material B was reduced from 0.20 mN to 0.13 mN, a 35% reduction in capillary force. which changed the distribution of droplets on the fibres and enhanced the wettability of the filter material. This discovery is of great significance for understanding the variation pattern of filter material performance under high pressure conditions, and provides a basis for the design and manufacture of filter elements for high pressure occasions.
Apolipoprotein H as a Crucial Immunomodulatory Factor in Sepsis
Authors: Yu Zhicai , Xiao Changxue , Liu Rong , Pi Dandan , Jin Bian , Zou Zhen , Xu Feng 
Published: September 2023

Abstract
Background.
Sepsis is a life-threatening acute organ dysfunction caused by a dysregulated immune response to infections. Apolipoprotein H (APOH) is an important plasma protein that regulates diverse biological processes. However, the role of APOH in the immunopathology of sepsis remains unidentified.
Methods.
APOH concentration was determined in pediatric patients with sepsis and healthy individuals. The effect of APOH on survival, organ injury, and inflammation were measured in an experimental sepsis model of cecal ligation and puncture (CLP). The possible mechanisms of APOH on anti-inflammation response were explored in PBMC, PM, BMDM and RAW 264.7 macrophages.
Results.
APOH concentration was significantly decreased in the patients with sepsis compared with those of healthy controls, and was negatively linked with severity and mortality of pediatric sepsis. Therapeutic administration of recombinant APOH protein reduced the mortality rate in mice with sepsis, alleviated organs injury and inhibited inflammation in mice with severe sepsis. Conversely, neutralizing APOH by anti-APOH monoclonal antibody led to an increased mortality rate, organs injury and inflammation in mice with nonsevere sepsis. Intriguingly, APOH had negligible influences on bacterial burden and the counts of neutrophils and macrophages in sepsis mice model, and bacterial phagocytosis and killing upon P. aeruginosa infection in PM, RAW 264.7 and PBMC cells. Mechanistic investigation in PM and RAW 264.7 cells indicated that APOH reshaped macrophage polarization by inhibiting M1 macrophage through suppression of toll-like receptor 4 (TLR4)/nuclear factor-κB (NF-κB) signaling pathway.
Conclusion.
This proof-of-concept study elucidated that APOH played an important role in the host defense response to sepsis and suggested a potential therapeutic role for APOH in the treatment of sepsis.
Using a Solvent-Induced Self-Assembly Approach to Fabricate and Tune the Organogels and Hydrogels
Authors: Wang Ruicong , Hao Xiaoting , Yang Haikuan 
Published: September 2023

Abstract
In the present work, stable organogels and hydrogels could be formed by dimeric-dehydrocholic acid derivative (DDAD) in different solvents. Compared with the organogels, the hydrogels formed by DDAD were found to be thermal reversible and had higher gel-to-solution transition temperature. The supramolecular structures in the organogels and hydrogels were further studied by using transmission electron microscopy (TEM) and atomic force microscopy (AFM). TEM and AFM images of the supramolecular gels showed that the solvent effects played a crucial role in morphological structures. Specifically, the organogel had a three-dimensional porous network structure. While, the hydrogel had a supramolecular structure made up of long fibers. Fourier transformation infrared spectroscopy (FT-IR) showed that multiple hydrogen bonds among the gelator molecules were the main driving forces in gel formation. On this base, the solvent effects on the gelation abilities and thermal stability were discussed. Thus, the present study provides a solvent-induced self-assembly approach and contributes substantially to the development of the supramolecular gels as soft materials.
Anaerobic fungi in the tortoise alimentary tract illuminate early stages of host-fungal symbiosis and Neocallimastigomycota evolution
Authors: Youssef Noha , Pratt Carrie , Meili Casey , Jones Adrienne , Jackson Darian , England Emma , Rogers Janet , Hartson Steve , Elshahed Mostafa , Wang Yan 
Published: September 2023

Abstract
The anaerobic gut fungi (AGF, Neocallimastigomycota) reside in the alimentary tract of herbivores. While their presence in mammals is well documented, evidence for their occurrence in non-mammalian hosts is currently sparse. Here we report on AGF communities in tortoises (family Testudinidae). Culture-independent surveys of tortoise fecal samples identified a unique AGF community, with three novel deep-branching genera representing > 90% of sequences in most samples. Representatives of all genera were successfully isolated under strict anaerobic conditions at 30ºC or 39ºC. Transcriptomics-enabled phylogenomic and molecular dating analysis indicated an ancient, deep-branching position in the AGF tree for these genera, with an evolutionary divergence time estimate of 104–112 million years ago (Mya). Such estimates push the establishment of animal- Neocallimastigomycota symbiosis from the early Paleogene (67 Mya) to the early Cretaceous (112 Mya). Further, compared to their mammalian counterparts, tortoise-associated isolates exhibited a more limited capacity for plant polysaccharides metabolism and lacked genes encoding several carbohydrate active enzyme (CAZyme) families mediating their degradation. Finally, we demonstrate that the observed curtailed degradation capacities and reduced CAZyme repretoire in tortoise-associated AGF is driven by the paucity of horizontal gene transfer (HGT) in tortoise-associated AGF genomes, compared to the massive HGT occurrence in mammalian AGF taxa. The reduced CAZyome and overall secretory machinery observed is also reflected in an altered cellulosomal production capacity in tortoise-associated AGF. Our findings provide novel insights into the scope of phylogenetic diversity, ecological distribution, evolutionary history, evolution of fungal-host nutritional symbiosis, and dynamics of genes and traits acquisition in Neocallimastigomycota.
Evaluation of platelet lysate-based medium and protein substrate for HUVEC cell culture and expansion
Authors: Rojas Juan Manuel Duarte , Múnera Luz Marina Restrepo , Mira Sergio Estrada 
Published: September 2023

Abstract
Endothelial cell (EC) culture requires specialized and commercial culture media that differ fundamentally in the composition of growth supplements. These media are expensive and must be imported, increasing the time to effective use. Human platelet lysate (PL) and platelet lysate serum (PLS) media are emerging alternatives to commercial media. This study evaluated the performance and behavior of human umbilical vein endothelial cells (HUVEC) using the commercial medium ECGM-Promocell® and media supplemented with PL and PLS. After obtaining informed consent, umbilical cords were collected and HUVEC were isolated. The performance of HUVECs was compared between ECGM-Promocell® commercial medium and media developed from PL and PLS. A PL-derived protein substrate was introduced to pre-treat and form a thin layer on culture plates. The cells were characterized by flow cytometry using the markers CD31, CD144, CD146, CD34, and HLA-DR. A mixed culture medium was obtained from the combination of PLS and ECGM-Promocell® commercial medium, which was able to maintain the viability, adhesion, and proliferation of ECs. At the same time, a protein substrate was implemented using PL, which was added to the surface of the culture plates, being able to simulate an extracellular matrix, facilitating enhanced endothelial cell adhesion, proliferation, and yield. Cells cultured with ECGM-Promocell® and the mixed medium, with and without the PL protein substrate, expressed the surface markers CD31, CD144, and CD146, and were negative for CD34 and HLA-DR markers. The mixed medium together with the PL protein substrate represents excellent alternatives for the culture, maintenance, and proliferation of endothelial cells; being a promising and profitable strategy for the research and production of these cells for therapeutic and research purposes.
Cloning of PGRP Gene and Its Expression Responding to the Stimulations of Vibrio Alginolyticus, LPS, and Poly (I:C) in Pinctada fucata
Authors: Zhang Shengyu , Yu Dahui , Liang Xia , Jiang Haoxin , Li Suping , Yan Xueyu , Wang Pei , Guo Ying , Zhai Ziqin , Bai Lirong 
Published: September 2023

Abstract
The PfPGRP gene from the pearl oyster Pinctada fucata was cloned and expressed in different tissues with and without stimulation by Vibrio alginolyticus, LPS, and Poly (I:C) to investigate its role in the immune response. The cDNA sequence of PfPGRP was 789 bp long, containing an open reading frame of 591 bp that encoded 196 amino acids. Phylogenetic analysis showed that the genetic distance between vertebrate and invertebrate PGRPs was significant, and the homology of PGRP gene sequences in mollusks was around 50%. The PfPGRP gene had the highest homology with the PGRP gene from Crassostrea gigas, at 72.9%. Protein domain prediction identified a conserved canonical PGRP domain and an Ami_2 domain in PfPGRP, suggesting that it could hydrolyze invading bacteria and terminate the immune response. Quantitative PCR showed that PfPGRP was expressed constitutively in all tissues, with the highest levels in the mantle, followed by the gonad and hepatopancreas. The expression of PfPGRP in the mantle was significantly upregulated after LPS injection, indicating that this tissue is more sensitive to LPS and plays a crucial role in defending against bacterial invasions. Similarly, the expression of PfPGRP in the hepatopancreas was significantly upregulated after both Vibrio and LPS injections, but reached its maximum later in the Poly (I:C) group, suggesting that it is more sensitive to bacteria and may be involved in bacterial elimination.Overall, these results suggest that PfPGRP plays an essential role in the innate immune system of P. fucata and is involved in defense against bacterial and viral invasions.
Managing a difficult airway due to supraglottic masses: successful videolaryngoscopic intubation after induction of general anesthesia
Authors: Jeong Hye-won , Song Eun-Jin , Jang Eun-A , Kim Joungmin 
Published: September 2023

Abstract
Background Awake fiberoptic intubation is regarded as the gold standard for managing anticipated difficult airways. However, it may not be feasible in patients with supraglottic masses.Case presentation A 41-year-old female patient was scheduled for excision of bilateral, mobile, and pedunculated masses on both aryepiglottic folds, which covered almost the entire upper part of the glottis. Considering her symptoms (neither stridor nor dyspnea in any position) and the risks of awake fiberoptic intubation in this patient (bleeding, damage, and displacement of the masses), the initial decision was to proceed with gentle tracheal intubation using a videolaryngoscope under general anesthesia. Ensuring that mask ventilation was maintained with ease, the patient was sequentially administered intravenous propofol, remifentanil, and rocuronium. Under sufficient depth of anesthesia, intubation using a videolaryngoscope was successfully performed without any complications.Conclusions Videolaryngoscopic intubation after induction of general anesthesia can be a feasible alternative for managing difficult airways in patients with supraglottic masses.
Relationship between the lipidome profile and disease activity in patients with rheumatoid arthritis
Authors: Masuoka Shotaro , Nishio Junko , Yamada Soichi , Saito Kosuke , Kaneko Kaichi , Kaburaki Makoto , Tanaka Nahoko , Sato Hiroshi , Muraoka Sei , Kawazoe Mai , Mizutani Satoshi , Ishii-Watabe Akiko , Kawai Shinichi , Saito Yoshiro , Nanki Toshihiro 
Published: September 2023

Abstract
Lipid mediators have been suggested to play important roles in the pathogenesis of rheumatoid arthritis (RA). Lipidomics has recently allowed for the comprehensive analysis of lipids and has revealed the potential of lipids as biomarkers for the early diagnosis of RA and prediction of therapeutic responses. However, the relationship between disease activity and the lipid profile in RA remains unclear. In the present study, we performed a plasma lipidomic analysis of 278 patients with RA during treatment and examined relationships with disease activity using the Disease Activity Score in 28 joints (DAS28)-erythrocyte sedimentation rate (ESR). In all patients, 12 lipids positively correlated and 7 lipids negatively correlated with DAS28-ESR. Stearic acid [FA(18:0)] (r = -0.45) and palmitic acid [FA(16:0)] (r = -0.39) showed strong negative correlations. After adjustments for age, body mass index (BMI), and medications, stearic acid, palmitic acid, bilirubin, and lysophosphatidylcholines negatively correlated with disease activity. Stearic acid inhibited osteoclast differentiation from peripheral blood monocytes in in vitro experiments, suggesting its contribution to RA disease activity by affecting bone metabolism. These results indicate that the lipid profile correlates with the disease activity of RA and also that some lipids may be involved in the pathogenesis of RA.
Predicting the spatio-temporal dynamics of Popillia japonica populations
Authors: Gilioli Gianni , Sperandio Giorgio , Simonetto Anna , Ciampitti Mariangela , Cavagna Beniamino , Bianchi Alessandro , Battisti Andrea , Mori Nicola , De Francesco Andrea , Gervasio Paola 
Published: September 2023

Abstract
We developed a reaction-diffusion model to describe the spatio-temporal dynamics of the Japanese beetle (Popillia japonica Newman), based on adult abundance data collected by the Regional Phytosanitary Service during the monitoring activities in the infested area in Lombardy (northern Italy), from 2015 to 2021. The model simulated the abundance of the pest, with a discrete time step of one year along linear trajectories departing from an initial point of establishment. The model allowed to determine the asymptotic spreading speed (i.e., the speed at which the leading edge of a population wave moves over time) of the pest along 13 different trajectories, and varied from 4.5 to 13.8 km/y, with a mean value of 8.2 km/y. Finally, we developed a land suitability index that summarises the effect of land use on the trajectory-specific asymptotic spreading speed of P. japonica. Specifically, it has been observed an increase in the spreading speed of 260 meters per year for an increase of a percentage point of the land suitability index. The model presented and the knowledge acquired in this work represent an important step forward in the comprehension of P. japonica population dynamics, and they represent important elements for the development of a decision support tool for pest risk managers to design and implement scientifically-driven management actions.
“That’s why we’re speaking up today.”: Exploring Barriers to Overdose Fatality Prevention in Indianapolis’ Black Community with Semi-Structured Interviews
Authors: Seo Dong-Chul , Satterfield Naomi , Alba-Lopez Leonardo , Lee Shin Hyung , Crabtree Charlotte , Cochran Nicki 
Published: September 2023

Abstract
Background: Opioid overdose deaths are of great concern to public health, with over one million lives lost since 1999. While many efforts have been made to mitigate these, Black communities continue to experience a greater burden of fatalities than their white counterparts. This study aims to explore why by working with members of low-income primarily Black communities in Indianapolis through semi-structured interviews.
Methods: Semi-structured one-on-one in-depth interviews were conducted in spring and summer of 2023 with Black residents (N=23) of zip codes 46202, 46205, 46208, and 46218 in Indianapolis. Ten interview questions were used to facilitate conversations about opioid overdoses, recovery, fatality prevention tools such as calling 911 and naloxone, law enforcement, and racism. Data were analyzed using grounded theory and thematic analysis.
Results: Interviews revealed access barriers and intervention opportunities. Racism was present in both. Mental access barriers such as stigma, fear, and mistrust contributed to practical barriers such as knowledge of how to administer naloxone. Racism exacerbated mental barriers by adding the risk of race-based mistreatment to consequences related to association with substance use. Participants discussed the double-stigma of substance use and being Black, fear of being searched in law enforcement encounters and what would happen if law enforcement found naloxone on them, and mistrust of law enforcement and institutions that provide medical intervention. Participants had favorable views of interventions that incorporated mutual aid and discussed ideas for future interventions that included this framework.
Conclusions: The impact mental barriers have on access to opioid overdose prevention behaviors and tools, such as calling 911 and administering naloxone, are magnified by racism. Information and resources coming from people within marginalized communities tend to be trusted. Leveraging inter-community relationships may increase engagement in opioid overdose fatality prevention. Interventions and resources directed towards addressing opioid overdose fatalities in Black communities should use mutual aid frameworks to increase the utilization of the tools they provide.
More accuracy approach for signal subspace-based algorithms in bistatic EMVS-MIMO radar
Authors: Xue Fengtao , Yang Yunxiu , Feng Maoyuan , Shu Qin 
Published: September 2023

Abstract
In this article, we rediscuss the parameter estimation in a bistatic multiple-input multiple-output radar system with electromagnetic vector sensor (EMVS) and propose an improved approach for all signal subspace-based estimation algorithms. First, the signal subspace is obtained by directly performing SVD or high-order SVD on the signal matrix or the signal tensor. Then, the elevation angle is automatically estimated by exploiting the rotation invariance of the receive-transmit array manifold and the Joint diagonalization technology. Then, the estimated elevation angle is taken as prior information, and the spatial response vector is recovered from the entire signal subspace using least-squares by exploring the property of the Kronecker product. Next, the azimuth angle is estimated by the ‘Vector Cross-Product’ strategy. Finally , the polarization parameters are calculated based on the least-squares method. The proposed algorithm is analyzed from the aspects of identifi-ability and complexity. The proposed signal subspace acquisition method is less computationally intensive. The improved parameter estimation approach can realize automatic parameter pairing and have a better parameter estimation performance than the previous corresponding algorithms when it works on the subspace obtained in different ways. Simulation results verify the performance improvement of the proposed algorithm.