bluuebunny/crossref_metadata_2025_split huggingface dataset:
Transcranial Direct Current Stimulation Reverses Stroke-Induced Network Alterations in Mice
Authors: Blaschke Stefan J. , Vlachakis Susan , Pallast Niklas , Walter Helene L. , Volz Lukas J. , Wiedermann Dirk , Fink Gereon R. , Hoehn Mathias , Aswendt Markus , Schroeter Michael , Rueger Maria A. 
Published: June 2023

BACKGROUND:
Beyond focal effects, stroke lesions impact the function of distributed networks. We here investigated (1) whether transcranial direct current stimulation (tDCS) alters the network changes induced by cerebral ischemia and (2) whether functional network parameters predict the therapeutic efficacy of tDCS in a mouse model of focal photothrombotic stroke.

METHODS:
Starting 3 days after stroke, cathodal tDCS (charge density=39.6 kC/m²) was applied over 10 days in male C57Bl/6J mice under light anesthesia over the lesioned sensory-motor cortex. Functional connectivity (resting-state functional magnetic resonance imaging) was evaluated for up to 28-day poststroke, with global graph parameters of network integration computed.

RESULTS:
Ischemia induced a subacute increase in connectivity accompanied by a significant reduction in characteristic path length, reversed by 10 days of tDCS. Early measures of functional network alterations and the network configuration at prestroke baseline predicted spontaneous and tDCS-augmented motor recovery.

DISCUSSION:
Stroke induces characteristic network changes throughout the brain that can be detected by resting-state functional magnetic resonance imaging. These network changes were, at least in part, reversed by tDCS. Moreover, early markers of a network impairment and the network configuration before the insult improve the prediction of motor recovery.
Efficacy of Angioscopy for the Detection of Mobile Carotid Artery Lesions
Authors: Fukutome Kenji , Aketa Shuta , Mitsui Takaaki , Shiraishi Yuki , Hayami Hiromichi , Murakami Yasutaka , Matsuoka Ryuta , Shiba Mikio , Tei Rinsei , Shin Yasushi , Motoyama Yasushi 
Published: June 2023

Case presentation
A 78‐year‐old man with a mobile lesion was diagnosed with thrombus using carotid ultrasonography, but the lesion was not completely resolved with dual antiplatelet and anticoagulation therapy. Direct visualization by angioscopy showed a white mobile plaque. The carotid artery was stented with a double‐layered stent, as the plaque persisted despite continuing the medical treatment and was linked to an increased risk of cerebral embolism. The plaque was attached to the arterial wall, and it subsequently disappeared. The patient recovered well and no further emboli were observed.

Conclusions
Angioscopy is effective for identifying lesions under direct vision. The characteristics and dynamics of plaques may be viewed via angioscopy, which aids in making treatment‐related decisions, particularly in the case of carotid artery plaques.
Maupertuis and the Reshaping of Natural History in Eighteenth-Century France
Authors: Storni Marco 
Published: June 2023

Abstract
In this paper, I analyze a momentous change in eighteenth century French life sciences. Whereas in the first half of the century the conception of natural history as the systematic collection of facts had been most successful, at mid-century a new approach emerged. This approach was characterized by an accent on general philosophical themes rather than on observation and experiment. I study the grounds and features of this historical shift through the work of Maupertuis, who published papers in institutional (or Baconian) natural history in his early years, but then adopted a more speculative approach to the life sciences.
Richard Lavenham’s Tractatus terminorum naturalium
Authors: Hanke Miroslav 
Published: June 2023

Abstract
The late fourteenth-century English Carmelite Richard Lavenham was a prolific author of Latin and vernacular treatises on logic, physics, philosophy, and theology. Among other works pertaining to natural philosophy, he authored the short Tractatus terminorum naturalium, preserved in three complete or almost complete late fourteenth- or early fifteenth-century copies, with the opening passage preserved in three other manuscripts. The text is fundamentally a redaction of the Heytesburian Termini naturales, a brief glossary of technical vocabulary of the natural philosophy and physics of the Oxford Calculators, supplemented by additional textual and pictorial material. As such, Lavenham’s treatise is a specific branch of an influential tradition of school texts that took the form of elementary treatises, additional glosses, quaestio-commentaries, and excerpts.
Local Communities and Archaeological Sites in Tunisia: A Case Study at Dougga (Ancient Thugga) about Cultural Memory and Cultural Markers in the Longue Durée
Authors: Whitehouse John , Harize Sami 
Published: June 2023

Abstract
The experience and legends of local communities in Tunisia who lived within archaeological sites and shared long-standing connections with those sites has been poorly recognised and documented. The extent to which elements of the cultural memory of local communities refer back to the pre-Islamic period has often been hinted at, but rarely explored in detail. The aim of this paper is to examine the relationship between local communities and archaeological sites in Tunisia, and the extent to which modern cultural memory in a community embeds elements or cultural markers from the Roman, Punic and Numidian past. This case study examines the cult of Oum Khoula at the site of Dougga (ancient Thugga). The marabout of Oum Khoula is in the cisterns of Aïn El-Hammam, at the end of the major Roman aqueduct to Dougga. Oum Khoula is remembered by local inhabitants as associated with a range of legends stretching back to the Roman and pre-Roman past. The cult has continued to be revered to the modern day. Whether the cultural memory illustrated by the cult of Oum Khoula at Dougga represents continuity with the past cannot be proved or disproved and is ultimately not the important issue. The cult of Oum Khoula at Dougga represents an example of the persistence of references from Numidian, Punic and Roman periods in the Maghreb. That cultural memory is a function of the community’s sense of place and significant references to that place derive from the archaeological remains and its associated oral traditions and symbols found at Dougga. The question of how modern archaeologists respond to and interpret this cultural memory is discussed.
The Dangers of Desire: Medieval Franciscans as Book Owners
Authors: Welch Anna 
Published: June 2023

Abstract
The Order of the Friars Minor faced a dilemma from the outset: their Rule of life, written by their founder St Francis of Assisi, included a strict adherence to the ideal of absolute poverty (that is, living without property, even property held in common), and yet it was impossible to fulfil their pastoral activities in the community without at least one type of property – liturgical books. In both the earlier (1209/10–1221) and later (1223) Rules, St Francis discussed the ownership of books, but not their production. Surviving manuscripts and archival records alert us to the fact that friars did produce their own liturgical books and act as the scribes and illuminators of books made for others. As both producers and owners of illuminated manuscripts, the friars engaged in a careful navigation of the relationship between beauty (permissible as an expression of invisible divine beauty, as defined by Hugh of St Victor) and luxury. It was all too easy (as the Franciscan Durand of Champagne bemoaned) for friars to desire ‘beautiful … and … curiously illuminated [books, rather] than true and well corrected ones’. This essay explores the ways in which friars negotiated the issues of poverty, beauty and luxury, and how they expressed and satisfied their desire for books, drawing on examples from the library of the Sacro Convento in Assisi.
When Churches Discovered the Digital Divide: Overcoming Technological Inaccessibility, Hesitancy & Digital Reluctance During the covid-19 Pandemic
Authors: Campbell Heidi A 
Published: June 2023

Abstract
During the pandemic, many church leaders had to learn to manage a digital divide present within their congregations, which they were not aware of. This article explores the nature and traits of the digital divide many American churches encountered during this pandemic, based on a study of focus groups involving 478 church leaders from Indiana. This study identifies four specific challenges they faced in adopting digital media and adapting their services to the online environment, revealed by this digital divide. Specifically, this article explores the lack of internet accessibility digital literacy, generational gaps in digital knowledge, and dealing with in and dividuals who were resistant to change digital media, who could be described as exhibiting technological hesitancy or digital reluctance. These challenges are looked at through the lens of digital justice and argue the digital divide realized within churches during the Covid-19 pandemic has theological implications.
Tiberian Dagesh Lene
Authors: Howard Jonathan 
Published: June 2023

Abstract
In the previous installment of Textus, Yehonatan Wormser acutely argued that the so-called dagesh lene in the standard Tiberian tradition was pronounced as a geminate consonant, effectively neutralising the distinction between lene and forte. This he did by invoking the “principle of economy” and comparing the dagesh to the shewa, as well as by drawing evidence from Karaite transcriptions and non-standard Tiberian manuscripts. I argue that his claim is not supported by his evidence: the transcriptions and non-standard manuscripts do not reflect the mainstream Tiberian tradition, but rather a late development; and the principle of economy does not apply in the cases of complementary distribution. Wormser’s analogy between dagesh and shewa is also addressed.
Readmission and associated clinical factors among individuals admitted with bipolar affective disorder at a psychiatry facility in Uganda
Authors: Abaatyo Joan , Kaggwa Mark Mohan , Favina Alain , Olagunju Andrew T. 
Published: June 2023

Abstract
Background
Bipolar affective disorder (BAD) is a common severe mental health condition with a relapsing course that may include periods of hospital re-admissions. With recurrent relapses and admissions, the course, prognosis, and patient’s overall quality of life can be affected negatively. This study aims to explore the rates and clinical factors associated with re-admission among individuals with BAD.

Method
This study used data from a retrospective chart review of all records of patients with BAD admitted in 2018 and followed up their hospital records for four years till 2021 at a large psychiatric unit in Uganda. Cox regression analysis was used to determine the clinical characteristics associated with readmission among patients diagnosed with BAD.

Results
A total of 206 patients living with BAD were admitted in 2018 and followed up for four years. The average number of months to readmission was 9.4 (standard deviation = 8.6). The incidence of readmission was 23.8% (n = 49/206). Of those readmitted during the study period, 46.9% (n = 23/49) and 28.6% (n = 14/49) individuals were readmitted twice and three times or more, respectively. The readmission rate in the first 12 months following discharge was 69.4% (n = 34/49) at first readmission, 78.3% (n = 18/23) at second readmission, and 87.5% (n = 12/14) at third or more times. For the next 12 months, the readmission rate was 22.5% (n = 11/49) for the first, 21.7% (n = 5/23) for the second, and 7.1% (n = 1/14) for more than two readmissions. Between 25 and 36 months, the readmission rate was 4.1% (n = 2/49) for the first readmission and 7.1% (n = 1/14) for the third or more times. Between 37 and 48 months, the readmission rate was 4.1% (n = 2/49) for those readmitted the first time. Patients who presented with poor appetite and undressed in public before admission were at increased risk of being readmitted with time. However, the following symptoms/clinical presentations, were protective against having a readmission with time, increased number of days with symptoms before admission, mood lability, and high energy levels.

Conclusion
The incidence of readmission among individuals living with BAD is high, and readmission was associated with patients’ symptoms presentation on previous admission. Future studies looking at BAD using a prospective design, standardized scales, and robust explanatory model are warranted to understand causal factors for hospital re-admission and inform management strategies.
Knowledge and attitudes of Chinese medical postgraduates toward research ethics and research ethics committees: a cross-sectional study
Authors: Liu Xing , Wang Xianxiong , Wu Ying , Yu Haitao , Yang Min , Khoshnood Kaveh , Luo Esther , Wang Xiaomin 
Published: June 2023

Abstract
Background
Research ethics provides the ethical standards for conducting sound and safe research. The field of medical research in China is rapidly growing and facing various ethical challenges. However, in China, little empirical research has been conducted on the knowledge and attitudes of medical postgraduates toward research ethics and RECs. It is critical for medical postgraduates to develop a proper knowledge of research ethics at the beginning of their careers. The purpose of this study was to assess the knowledge and attitudes of medical postgraduates toward research ethics and RECs.

Methods
This cross-sectional study was conducted from May to July 2021 at a medical school and two affiliated hospitals in south-central China. The instrument of the study was an online survey that was distributed via WeChat.

Results
We found that only 46.7% were familiar with the ethical guidelines for research with human subjects. In addition, 63.2% of participants were familiar with the RECs that reviewed their research, and 90.7% perceived RECs as helpful. However, only 36.8% were fully aware of the functions of RECs. In the meantime, 30.7% believed that review by an REC would delay research and make it more difficult for researchers. Furthermore, most participants (94.9%) believed that a course on research ethics should be mandatory for medical postgraduates. Finally, 27.4% of the respondents considered the fabrication of some data or results to be acceptable.

Conclusion
This paper serves to suggest that research ethics education should be prioritized in medical ethics curriculum, and course syllabi or teaching methods should be revised to provide medical postgraduates with a deeper understanding of the principles, regulations, and specifics of research ethics. We also recommend that RECs provide diverse approaches in their review procedure to facilitate the understanding of medical postgraduates of the functions and processes of RECs and to enhance their awareness of research integrity.
Evaluation of a multiphasic parasite clearance profile after treatment of experimental human infection with the investigational anti-malarial M5717 using segmented mixed effect models
Authors: Yin Xiaoyan , Li Ying , Bagchus Wilhelmina , Yalkinoglu Özkan , Bezuidenhout Deon , Tappert Aliona , McCarthy James , Marquart Louise , Oeuvray Claude 
Published: June 2023

Abstract
Background
Evaluation of parasite clearance patterns in experimental human infection trials helps increase understanding of drug action. In a previously reported phase Ib trial of a new investigational anti-malarial drug M5717, parasite clearance showed a biphasic linear pattern: slow removal phase with a near flat clearance rate followed by a fast clearance phase with a steep slope. In this study three statistical approaches were implemented and compared to estimate the parasite clearance rate for each phase and the time point corresponding to the change of clearance rates (changepoint between the two phases).

Methods
Data using three M5717 doses 150 mg (n = 6), 400 mg (n = 8), 800 mg (n = 8) were used to estimate biphasic clearance rates. Three models were investigated: firstly, segmented mixed models with estimated changepoint—models with/without random effects in various parameters were compared. Secondly, a segmented mixed model using grid search—this method is similar to the first except that changepoints were not estimated, instead they were selected based on model fit from given candidate values. Thirdly, a two-stage approach whereby a segmented regression model fit to each participant followed by a meta-analysis method. Hourly rate of parasite clearance (HRPC) interpreted as the percentage of parasites removed each hour was calculated.

Results
The three models generated similar results. Using segmented mixed models, the estimated changepoints after treatment in hours (95% CI) were: 150 mg: 33.9 (28.7, 39.1); 400 mg: 57.4 (52.5, 62.4); and 800 mg: 52.8 (47.4, 58.1). For all three treatment groups, there was nearly no clearance before the changepoints, but rapid clearance in the second phase (HRPC [95% CI]): 150 mg: 16.8% (14.3, 19.1%); 400 mg: 18.6% (16.0, 21.1%); and 800 mg: 11.7% (9.3, 14.1%).

Conclusions
All three statistical approaches are effective tools to characterize the bi-phasic clearance of M5717 in the phase 1b experimental Plasmodium falciparum malaria human infection study. The statistical approaches produced similar results to estimate the two-phase clearance rates and the changepoint for each treatment dose of M5717. However, the segmented mixed model with random changepoints has several advantages: it is computationally efficient, provides precision for changepoint estimates and is robust concerning outlying datapoints or individuals.
Transcriptome sequencing and miRNA-mRNA network construction in exosome of macrophage M2 in stomach adenocarcinoma
Authors: Pan Dun , Li Zhipeng , Lin Xin , Li Liangqing 
Published: June 2023

Abstract
Background
Stomach adenocarcinoma (STAD) is the most common histological type of gastric cancer (GC). Macrophages are an essential part of the tumor microenvironment. We attempted to search for potential molecular markers associated with macrophages, which might be helpful for STAD diagnosis and treatment.

Methods
Firstly, exosome in macrophages was extracted for RNA sequencing to identify differentially expressed microRNAs (miRNAs) (DEmiRNAs). Then, DEmiRNAs and differentially expressed mRNAs (DEmRNAs) were screened in the Cancer Genome Atlas (TCGA) database. The miRNAs related to macrophage M2 polarization were obtained by intersecting the DEmiRNAs obtained from the sequencing data and TCGA data. Using the Pearson correlation coefficient method, the mRNAs significantly related to macrophage M2 were screened out, followed by construction of the macrophage M2-miRNA-mRNA network. Subsequently, real-time-polymerase chain reaction (RT-PCR) and online datasets were applied to validate the expression of DEmiRNAs and DEmRNAs.

Results
A total of 6 DEmiRNAs were identified in RNA sequencing; 59 DEmiRNAs and 1838 DEmRNAs were identified in TCGA database. Among which, a common miRNA (hsa-miR-133a-3p) associated with the M2 polarization of macrophages was identified. Fifteen common mRNAs were obtained between DEmRNAs and mRNAs targeted by DEmiRNAs. Eventually, a core macrophage M2-1 down-regulated miRNA-7 and up-regulated mRNAs network was constructed, including hsa-miR-133a-3p, SLC39A1, TTYH3, HAVCR2, TPM3, XPO1, POU2F1, and MMP14. The expression of miRNA and mRNAs was in line with the validation results of RT-PCR and online datasets.

Conclusion
In this study, the screening of biomarkers in exosome of macrophage M2 may contribute to the prognosis of STAD patients.
Genetic characterization of HIV-1 viruses among cases with antiretroviral therapy failure in Suzhou City, China
Authors: Dong Zefeng , Xu Zhihui , Zhou Ying , Tian Runfang , Zhou Kai , Wang Di , Ya Xuerong , Shen Qiang 
Published: June 2023

Abstract
Background
This retrospective study aimed to characterize the distribution of HIV-1 genotypes and the prevalence of drug resistance mutations in people with antiretroviral treatment (ART) failure in Suzhou City, China.

Methods
Pol gene of HIV-1 viruses in blood samples of EDTA anticoagulants from 398 patients with failed antiviral treatment was successfully amplified by using an in-house assay. Drug resistance mutations were analyzed by using the Stanford HIV Drug Resistance Database system (https://hivdb.stanford.edu/hivdb/by-mutations/). HIV-1 genotypes were determined by the REGA HIV subtyping tool (version 3.46, https://www.genomedetective.com/app/typingtool/hiv). Near full-length genomes (NFLG) of HIV-1 viruses were obtained by next generation sequencing method.

Results
Sequences analysis of the pol gene revealed that CRF 01_AE (57.29%, 228/398) was the dominant subtype circulating in Suzhou City, followed by CRF 07_BC (17.34%, 69/398), subtype B (7.54%, 30/398), CRF 08_BC (6.53%, 26/398), CRF 67_01B (3.02%, 12/398) and CRF55_01B (2.51%, 10/398). The overall prevalence of drug-resistant mutations in cases with ART failure was 64.57% (257/398), including 45.48% (181/398) for nucleotide reverse transcriptase inhibitors (NRTIs) mutations, 63.32% (252/398) for non-nucleoside reverse transcriptase inhibitors (NNRTIs) mutations, and 3.02% (12/398) for protease inhibitors (PIs) mutations. Ten near full-length genomes (NFLG) of HIV-1 viruses were identified, including six recombinants of CRF 01_AE and subtype B, two recombinants of CRF 01_AE, subtype B and subtype C sequences, one recombinant of CRF 01_AE and subtype C and one recombinant of CRF 01_AE, subtype A1 and subtype C.

Conclusions
The high prevalence of drug-resistant HIV-1 viruses was a serious challenge for HIV prevention and treatment of people with HIV infection. Treatment regimens for ART failure patients should be adjusted over time based on the outcome of drug resistance tests. NFLG sequencing facilitates the identification of new recombinants of HIV-1.
Gender differences in prevalence and associated factors of metabolic syndrome in first-treatment and drug-naïve schizophrenia patients
Authors: Zeng Kuan , Wang Shuo , Zhang Lin , Zhang Yanting , Ma Jun 
Published: June 2023

Abstract
Background
Metabolic syndromes (MetS) are clinical syndromes involving multiple pathological states with distinct gender-specific clinical patterns. As a serious disorder associated with psychiatric conditions, the prevalence of MetS is significantly higher in the population with schizophrenia (Sch). The aim of this paper is to report gender differences in the prevalence, associated factors and severity-related factors of MetS in first-treatment and drug-naïve (FTDN) patients with Sch.

Methods
A total of 668 patients with FTDN Sch were included in this study. We collected socio-demographic and general clinical information on the target population, measured and evaluated common metabolic parameters and routine biochemical indicators, and assessed the severity of psychiatric symptoms using Positive and Negative Symptom Scale (PANSS).

Results
In the target group, the prevalence of MetS was significantly higher in women (13.44%, 57/424) than in men (6.56%, 16/244). In the males, waist circumference (WC), fasting blood glucose (FBG), diastolic blood pressure (DBP), and triglycerides (TG) were risk factors for MetS, while systolic blood pressure (SBP), TG, total cholesterol (TC), low-density lipoprotein cholesterol (LDL-C), and platelet (PLT) were risk factors for the females. More importantly, for the females, we found that age, LDL-C, PANSS scores and blood creatinine (CRE) were risk factors for higher MetS scores, while onset age and hemoglobin (HGB) were protective factors.

Conclusion
There are significant gender differences in the prevalence of MetS and its factors among patients with FTDN Sch. The prevalence of MetS is higher and the factors that influence MetS are more numerous and extensive in females. The mechanisms of this difference need further research and clinical intervention strategies should be formulated with gender differences.