bluuebunny/crossref_metadata_2025_split huggingface dataset:
Laparoscopic‐assisted thoracoscopic repair of latent traumatic diaphragmatic hernia: A case report
Authors: Yoshimine Sota , Tanaka Toshiki , Murakami Junichi , Yamamoto Naohiro , Kurazumi Hiroshi , Harada Eijiro , Hamano Kimikazu 
Published: August 2023

AbstractSurgical approaches for traumatic diaphragmatic hernia include transabdominal, transthoracic, and thoracoabdominal. Selection of the optimal approach depends on the timing and organ damage, often minimally invasive approaches with laparoscopy or thoracoscopy are performed. A 47‐year‐old man with blunt chest trauma was diagnosed with left traumatic diaphragmatic hernia 1 month after the trauma. The prolapsed omentum was detached from the chest wall and around the hernia orifice and returned to the abdominal cavity by coordinated thoracoscopic and laparoscopic manipulations. The 4 × 2 cm herniation in the diaphragm was sutured closed from the thoracic side while preventing re‐prolapse of the omentum and abdominal organs from the abdominal side. A combined thoracoscopic and laparoscopic approach can be effective in confirming organ damage, repositioning of prolapsed organs, and safe repair of the diaphragm in latent traumatic diaphragmatic hernia.
Direct and spillover effects of board gender quotas: Revisiting the Norwegian experience
Authors: Garcia‐Blandon Josep , Argilés‐Bosch Josep Maria , Ravenda Diego , Castillo‐Merino David 
Published: August 2023

AbstractBuilding on the Norwegian case, this study examines the long‐term implications of board gender quotas on the advancement of gender diversity in managerial leadership. Previous research has indicated that, aside from the board, the quota had limited impact on achieving this objective. However, these studies have narrowly focused on the spill‐over effects of the quota, primarily concentrating on the positions of CEO and Chair. The findings of this study reveal contrasting effects of the board gender quota on the gender composition of the board and the executive committee of the board. Consequently, Norwegian companies have increased the representation of women on their boards, as mandated by the law, while simultaneously experiencing a reduction in the presence of female executive directors. Moreover, the strength of both opposing effects has diminished over time. In addition to the board of directors, the quota has not influenced the promotion of gender diversity at other managerial levels. Furthermore, our study suggests that the quota has led to a decrease in the average tenure and level of independence of the boards, although it has not affected the qualifications of board members.
Neurofilament light chain: A potential biomarker for cerebrovascular disease in children with sickle cell anaemia
Authors: Green Nancy S. , Rosano Caterina , Bangirana Paul , Opoka Robert , Munube Deogratias , Kasirye Philip , Kawooya Michael , Lubowa Samson K. , Mupere Ezekiel , Conroy Andrea , Minja Frank J. , Boehme Amelia K. , Kang Min Suk , Honig Lawrence S. , Idro Richard 
Published: August 2023

SummaryCerebrovascular injury frequently occurs in children with sickle cell anaemia (SCA). Limited access to magnetic resonance imaging and angiography (MRI‐MRA) in sub‐Saharan Africa impedes detection of clinically unapparent cerebrovascular injury. Blood‐based brain biomarkers of cerebral infarcts have been identified in non‐SCA adults. Using plasma samples from a well‐characterized cross‐sectional sample of Ugandan children with SCA, we explored relationships between biomarker levels and MRI‐detected cerebral infarcts and transcranial Doppler (TCD) arterial velocity. Testing was performed using a 4‐plex panel of brain injury biomarkers, including neurofilament light chain (NfL), a central nervous system neuron‐specific protein. Mean biomarker levels from the SCA group (n = 81) were similar to those from non‐SCA sibling controls (n = 54). Within the SCA group, NfL levels were significantly higher in those with MRI‐detected infarcts compared to no infarcts, and higher with elevated TCD velocity versus normal velocity. Elevated NfL remained strongly associated with MRI‐detected infarcts after adjusting for sex and age. All non‐SCA controls and SCA participants lacking MRI‐detected infarcts had low NfL levels. These data suggest potential utility of plasma‐based NfL levels to identify children with SCA cerebrovascular injury. Replication and prospective studies are needed to confirm these novel findings and the clinical utility of NfL versus MRI imaging.
Clinical efficacy and safety of chimeric antigen receptor T‐cell therapy for mantle cell lymphoma with secondary central nervous system involvement
Authors: Ryan Christine E. , Zon Rebecca L. , Redd Robert , Fisher David C. , Shouval Roni , Kumar Anita , Crombie Jennifer L. , Sadrzadeh Hossein , Kim Austin I. , Nayak Lakshmi , Chukwueke Ugonma N. , Jacobson Caron A. , Frigault Matthew J. , Palomba M. Lia , Armand Philippe , Epstein‐Peterson Zachary , Merryman Reid W. 
Published: August 2023

SummaryData describing outcomes of chimeric antigen receptor (CAR) T‐cell therapy in patients with secondary central nervous system (SCNS) involvement of mantle cell lymphoma (MCL) are limited. We identified 10 patients with MCL and SCNS involvement treated with anti‐CD19 CAR T‐cell therapy at three US academic centres. Frequent objective responses were observed in the CNS (86%) and systemically (90%), and the 1‐year progression‐free survival was 47%. Seven patients developed immune‐effector‐cell‐associated‐neurotoxicity‐syndrome (n = 2 Grade 1, n = 5 Grade 3). Our results suggest that anti‐CD19 CAR T‐cell therapy in this setting is feasible and additional data regarding neurotoxicity in this population may be warranted.
Ten steps for ileoanal pouch anastomosis
Authors: Celentano Valerio , Manzo Carlo Alberto 
Published: August 2023

AbstractAimAppropriate patient selection, surgical technique, and follow‐up pathways can provide optimal functional outcomes and good quality of life in many patients undergoing ileoanal pouch surgery. The aim of this study was to demonstrate the standardised approach to ileoanal pouch formation that we have developed in our pouch surgery centre.MethodsWe developed a structured approach to laparoscopic proctectomy with ileoanal pouch anastomosis formation, divided into 10 different steps. All patients referred to our centre from January 2020 to December 2022 for ulcerative colitis were included in the study.ResultsA total of 38 consecutive patients underwent ileal pouch‐anal anastomosis (IPAA) surgery. All procedures were completed laparoscopically with one conversion to open (2.6%). A total of 13 patients had postoperative complications within 30 days of surgery (34.2%), with six (15.8%) being Clavien Dindo class 3 or higher. Median follow‐up length was 18 months (range 2–30). Median number of bowel movements in 24 h at 12 months post‐surgery was 4 (range 1–11).ConclusionsOur modular 10 steps approach could provide a standardised framework to surgeons in the learning curve. IPAA is a complex surgical procedure with significant postoperative morbidity. Our stepwise approach resulted in a high rate of minimally invasive surgery and could facilitate introduction of the technique.
The ASGLOS Study: A global survey on how predatory journals affect scientific practice
Authors: Martinino Alessandro , Puri Oshin , Pereira Juan Pablo Scarano , Owen Eloise , Chatterjee Surobhi , Abouelazayem Mohamed , Yang Wah , Mulita Francesk , Graham Yitka , Parmar Chetan , Ramnarain Dharmanand , Isik Arda , Yadav Shruti , Budihal Bhargavi R. , Kashyap Shankarsai , Aloulou Mohammad , Kundu Mrinmoy , Ibrahimli Arturan , Rajesh Eshwar , Silva Reewen George D. , Bhatt Gaurang , Malhotra Kashish , Magnani Riccardo , Smeenk Frank W. J. M. , Pouwels Sjaak 
Published: August 2023

AbstractPredatory journals and conferences are an emerging problem in scientific literature as they have financial motives, without guaranteeing scientific quality and exposure. The main objective of the ASGLOS project is to investigate the predatory e‐email characteristics, management, and possible consequences and to analyse the extent of the current problem at each academic level. To collect the personal experiences of physicians’ mailboxes on predatory publishing, a Google Form® survey was designed and disseminated from September 2021 to April 2022. A total of 978 responses were analysed from 58 countries around the world. A total of 64.8% of participants indicated the need for 3 or fewer emails to acquire a criticality view in distinguishing a real invitation from a spam, while 11.5% still have doubt regardless of how many emails they get. The AGLOS Study clearly highlights the problem of academic e‐mail spam by predatory journals and conferences. Our findings signify the importance of providing academic career‐oriented advice and organising training sessions to increase awareness of predatory publishing for those conducting scientific research.
An analysis of gender inclusion in Water, Sanitation and Hygiene (WASH) projects: Intention vs. reality
Authors: Robinson Hannah Jayne , Barrington Dani , Evans Barbara , Hutchings Paul , Narayanaswamy Lata 
Published: August 2023

AbstractMotivationGender equality is inherently bound with Water, Sanitation and Hygiene (WASH) provision, access, and use. Gender shapes experiences of projects and services, from participation in design to ensuring access to appropriate facilities. Many observers call for active attention to gender throughout the project cycle, but there is little evidence of the extent to which this happens in practice.PurposeThe article examines the extent to which evaluations of WASH implementation identify good gender‐inclusive practices. It explores the reasons for failings and suggests ways gender equality could be more actively considered and effected in WASH programming.Methods and approachTextual analysis was undertaken on World Bank and UNICEF project evaluation documents to identify how gendered elements were addressed. Practices were then categorized according to a Gender Sensitivity Framework, rating them on a sliding scale measure from “gender insensitive” to “transformative.” The perceived barriers to gendered programming were subsequently triangulated using a mixed methods survey of WASH practitioners which used self‐identified challenges to assess consensus moderation to triangulate perceived barriers to gendered programming.FindingsLack of clarity in conceptualizing gender results in poorly defined targets that are often insufficiently context specific. Consequently, project objectives are either reductionist, limiting progress on “gender” to easily quantifiable measures that fail to capture the varied lived realities of gendered experiences, or comprise vague qualitative statements that cannot be accurately assessed, leaving gender inclusion unaddressed.Policy implicationsGender is a social construct that is shaped by culture. Context‐specific understanding would support more nuanced gender‐inclusion objectives that could be monitored while also correlating with people's lived realities. Regular evaluation of gender guidance would ensure organizations' understanding and conceptualization of gender reflects the fluidity of society. Policy and practice interventions that guarantee the active involvement of multiple stakeholders and diverse voices would ensure that implementation is effective and evaluation is more accurate.
Neurological phenotype of adenosine deaminase 2 deficient patients: a cohort study
Authors: Verschoof Merelijne A. , van Meenen Laura C. C. , Andriessen M. Valérie E. , Brinkman Daniëlle M. C. , Kamphuis Sylvia , Kuijpers Taco W. , Leavis Helen L. , Legger G. Elizabeth , Mulders‐Manders Catharina M. , de Pagter Anne P. J. , Rutgers Abraham , van Well Gijs T. J. , Coutinho Jonathan M. , Hak A. Elisabeth , van Montfrans Joris M. , Klouwer Femke C. C. 
Published: August 2023

AbstractBackground and purposePatients with adenosine deaminase 2 (ADA2) deficiency can present with various neurological manifestations due to vasculopathies and autoinflammation. These include ischaemic and hemorrhagic stroke, but less clearly defined neurological symptoms have also been reported.MethodsIn this cohort study, patients with confirmed ADA2 deficiency from seven university hospitals in the Netherlands were included. The frequency and recurrence rates of neurological manifestations before and after initiation of tumor necrosis factor α (TNF‐α) inhibiting therapy were analyzed.ResultsTwenty‐nine patients were included with a median age at presentation of 5 years (interquartile range 1–17). Neurological manifestations occurred in 19/29 (66%) patients and were the presenting symptom in 9/29 (31%) patients. Transient ischaemic attack (TIA)/ischaemic stroke occurred in 12/29 (41%) patients and was the presenting symptom in 8/29 (28%) patients. In total, 25 TIAs/ischaemic strokes occurred in 12 patients, one after initiation of TNF‐α inhibiting therapy and one whilst switching between TNF‐α inhibitors. None was large‐vessel occlusion stroke. Two hemorrhagic strokes occurred: one aneurysmatic subarachnoid hemorrhage and one spontaneous intracerebral hemorrhage. Most neurological symptoms, including cranial nerve deficits, vertigo, ataxia and seizures, were caused by TIAs/ischaemic strokes and seldom recurred after initiation of TNF‐α inhibiting therapy.ConclusionsNeurological manifestations, especially TIA/ischaemic stroke, are common in patients with ADA2 deficiency and frequently are the presenting symptom. Because it is a treatable cause of young stroke, for which antiplatelet and anticoagulant therapy are considered contraindicated, awareness amongst neurologists and pediatricians is important. Screening for ADA2 deficiency in young patients with small‐vessel ischaemic stroke without an identified cause should be considered.
The Patient‐Determined Disease Steps scale is not interchangeable with the Expanded Disease Status Scale in mild to moderate multiple sclerosis
Authors: Foong Yi Chao , Merlo Daniel , Gresle Melissa , Zhu Chao , Buzzard Katherine , Lechner‐Scott Jeannette , Barnett Michael , Taylor Bruce , Kalincik Tomas , Kilpatrick Trevor , Darby David , Dobay Pamela , van Beek Johan , Hyde Robert , Butzkueven Helmut , van der Walt Anneke 
Published: August 2023

AbstractBackground and purposeThe validity, reliability, and longitudinal performance of the Patient‐Determined Disease Steps (PDDS) scale is unknown in people with multiple sclerosis (MS) with mild to moderate disability. We aimed to examine the psychometric properties and longitudinal performance of the PDDS.MethodsWe included relapsing–remitting MS patients with an Expanded Disability Status Scale (EDSS) score of less than 4. Validity and test–retest reliability was examined. Longitudinal data were analysed with mixed‐effect modelling and Cohen's kappa for concordance in confirmed disability progression (CDP).ResultsWe recruited a total of 1093 participants, of whom 904 had complete baseline data. The baseline correlation between PDDS and EDSS was weak (ρ = 0.45, p < 0.001). PDDS had stronger correlations with patient‐reported outcomes (PROs). Conversely, EDSS had stronger correlations with age, disease duration, Kurtzke's functional systems and processing speed test. PDDS test–retest reliability was good to excellent (concordance correlation coefficient = 0.73–0.89). Longitudinally, PDDS was associated with EDSS, age and depression. A higher EDSS score was associated with greater PDSS progression. The magnitude of these associations was small. There was no concordance in CDP as assessed by PDDS and EDSS.ConclusionThe PDDS has greater correlation with other PROs but less correlation with other MS‐related outcome measures compared to the EDSS. There was little correlation between PDDS and EDSS longitudinally. Our findings suggest that the PDDS scale is not interchangeable with the EDSS.
Clinical severity is correlated with age at seizure onset and biophysical properties of recurrent gain of function variants associated with SCN8A‐related epilepsy
Authors: Chung Kyung Mi , Hack Joshua , Andrews Jennifer , Galindo‐Kelly Maureen , Schreiber John , Watkins Joseph , Hammer Michael F. 
Published: August 2023

AbstractObjectiveGenetic variants in the SCN8A gene underlie a wide spectrum of neurodevelopmental phenotypes including several distinct seizure types and a host of comorbidities. One of the major challenges facing clinicians and researchers alike is to identify genotype–phenotype (G‐P) correlations that may improve prognosis, guide treatment decisions, and lead to precision medicine approaches.MethodsWe investigated G‐P correlations among 270 participants harboring gain‐of‐function (GOF) variants enrolled in the International SCN8A Registry, a patient‐driven online database. We performed correlation analyses stratifying the cohort by clinical phenotypes to identify diagnostic features that differ among patients with varying levels of clinical severity, and that differ among patients with distinct GOF variants.ResultsOur analyses confirm positive correlations between age at seizure onset and developmental skills acquisition (developmental quotient), rate of seizure freedom, and percentage of cohort with developmental delays, and identify negative correlations with number of current and weaned antiseizure medications. This set of features is more detrimentally affected in individuals with a priori expectations of more severe clinical phenotypes. Our analyses also reveal a significant correlation between a severity index combining clinical features of individuals with a particular highly recurrent variant and an independent electrophysiological score assigned to each variant based on in vitro testing.SignificanceThis is one of the first studies to identify statistically significant G‐P correlations for individual SCN8A variants with GOF properties. The results suggest that individual GOF variants (1) are predictive of clinical severity for individuals carrying those variants and (2) may underlie distinct clinical phenotypes of SCN8A disease, thus helping to explain the wide SCN8A‐related epilepsy disease spectrum. These results also suggest that certain features present at initial diagnosis are predictive of clinical severity, and with more informed treatment plans, may serve to improve prognosis for patients with SCN8A GOF variants.
In vitro response of dental pulp stem cells to dural substitute grafts: Analysis of cytocompatibility and bioactivity
Authors: Elhakim Ahmed , Kim Sunil , Shin Su‐Jung 
Published: August 2023

AbstractAimThe objective of this study was to assess and compare the cytocompatibility of decellularized porcine small intestine submucosal dural graft from Biodesign (BD) and polyester urethane‐based Neuro‐Patch (NP) dural substitute with the mineral trioxide aggregate (MTA) and the cyanoacrylate‐based Histoacryl surgical adhesive. Furthermore, the study evaluated the inflammatory response and osteogenic differentiation of human dental pulp stem cells (hDPSCs) when cultured in direct contact with the dural substitutes in comparison with MTA.MethodologyThe viability of hDPSCs in direct contact with the tested materials was investigated in vitro by a CCK‐8 assay. Additionally, the effects of dural substitutes and MTA on the expression of the inflammatory mediator interleukin‐6 (IL‐6) was investigated via enzyme‐linked immunosorbent assay (ELISA), whilst effects on the differentiation were evaluated using alizarin red staining, alkaline phosphatase staining, ELISA and energy‐dispersive X‐ray elemental mapping.ResultsThe dural substitutes were cytocompatible and promoted cellular adhesion. The Histoacryl and MTA demonstrated cytotoxicity in fresh preparations but showed a more favourable cellular reaction when set. Investigations of biological activity indicated that dural substitute membranes did not induce an inflammatory response or osteogenic differentiation of hDPSCs. In contrast, MTA induced the expression of IL‐6 and alkaline phosphatase activity contributing to enhanced differentiation and mineralization.ConclusionsThe dural substitute membranes showed cytocompatibility, did not provoke an inflammatory response and maintained the stemness of hDPSCs better than MTA. Additionally, the set Histoacryl surgical adhesive demonstrated good biocompatibility. Taken together, these results highlight the potential use of dural substitutes in regenerative endodontic procedures as coronal barriers alternative to MTA to reduce the incidence of intracanal calcifications.
Sleeping Beauty kit sets provide rapid and accessible generation of artificial antigen‐presenting cells for natural killer cell expansion
Authors: Dobson Lachlan J , Saunderson Sarah C , Smith‐Bell Samuel WJ , McLellan Alexander D 
Published: August 2023

AbstractArtificial antigen‐presenting cells (aAPCs) offer a cost effective and convenient tool for the expansion of chimeric antigen receptor (CAR)‐bearing T cells and NK cells. aAPCs are particularly useful because of their ability to efficiently expand low‐frequency antigen‐reactive lymphocytes in bulk cultures. Commonly derived from the leukemic cell line K562, these aAPCs lack most major histocompatibility complex expression and are therefore useful for NK cell expansion without triggering allogeneic T‐cell proliferation. To combat difficulties in accessing existing aAPC lines, while circumventing the iterative lentiviral gene transfers with antibody‐mediated sorting required for the isolation of stable aAPC clones, we developed a single‐step technique using Sleeping Beauty (SB)–based vectors with antibiotic selection options. Our SB vectors contain options of two to three genes encoding costimulatory molecules, membrane‐bound cytokines as well as the presence of antibiotic‐resistance genes that allow for stable transposition‐based transfection of feeder cells. Transfection of K562 with SB vectors described in this study allows for the surface expression of CD86, 4‐1BBL, membrane‐bound (mb) interleukin (IL)‐15 and mbIL‐21 after simultaneous transposition and antibiotic selection using only two antibiotics. aAPCs successfully expanded NK cells to high purity (80–95%). Expanded NK cells could be further engineered by lentiviral CAR transduction. The multivector kit set is publicly available and will allow convenient and reproducible in‐house production of effective aAPCs for the in vitro expansion of primary cells.