bluuebunny/crossref_metadata_2025_split huggingface dataset:
Impact of a positive end-expiratory pressure strategy on oxygenation, respiratory compliance, and hemodynamics during laparoscopic surgery in non-obese patients: a systematic review and meta-analysis
Authors: Arinova Gulfairus A. , Yukhnevich Yekaterina A. , Khamitova Zaukiya K. , Kim Sergey I. , Zhumabayev Murat B. , Berdiyarova Gulbanu S. , Shalekenov Sanzhar B. , Mukatova Irina Y. , Yaroshetskiy Andrey I. 
Published: May 2023

Abstract
Background.
Higher positive end-expiratory pressure (PEEP) during laparoscopic surgery may increase oxygenation and respiratory compliance. This meta-analysis aimed to compare the impact of different intraoperative PEEP strategies on arterial oxygenation, compliance, and hemodynamics during laparoscopic surgery in non-obese patients.
Methods.
We searched RCTs in PubMed, Cochrane Library, Web of Science, and Google Scholar from January 2012 to January 2022 comparing the different intraoperative PEEP (Low PEEP (LPEEP): 0 mbar; Moderate PEEP (MPEEP): 5–8 mbar; high PEEP (HPEEP): >8 mbar; individualized PEEP - iPEEP) on arterial oxygenation, respiratory compliance (Cdyn), mean arterial pressure (MAP), and heart rate (HR). We calculated mean differences (MD) with 95% confidence intervals (CI), and predictive intervals (PI) using random-effects models. The Cochrane Bias Risk Assessment Tool was applied.
Results.
21 RCTs (n = 1554) met the inclusion criteria. HPEEP vs LPEEP increased PaO2 (+ 29.38 [16.20; 42.56] mmHg, p < 0.0001) or PaO2/FiO2 (+ 36.7 [+ 2.23; +71.70] mmHg, p = 0.04). HPEEP vs MPEEP increased PaO2 (+ 22.00 [+ 1.11; +42.88] mmHg, p = 0.04) or PaO2/FiO2 (+ 42.7 [+ 2.74; +82.67] mmHg, p = 0.04). iPEEP vs MPEEP increased PaO2/FiO2 (+ 115.2 [+ 87.21; +143.20] mmHg, p < 0.001). MPEEP vs LPEP, and HPEEP vs MPEEP increased PaO2 or PaO2/FiO2 significantly with different heterogeneity. HPEEP vs LPEEP increased Cdyn (+ 7.87 [+ 1.49; +14.25] ml/mbar, p = 0.02). MPEEP vs LPEEP, and HPEEP vs MPEEP didn’t impact Cdyn (p = 0.14 and 0.38, respectively). iPEEP vs LPEEP decreased driving pressure (-4.13 [-2.63; -5.63] mbar, p < 0.001). No significant differences in MAP or HR were found between any subgroups.
Conclusion.
HPEEP and iPEEP during PNP in non-obese patients may improve oxygenation, increase Cdyn without clinically significant changes in MAP and HR. MPEEP could be insufficient to increase respiratory compliance and improve oxygenation. LPEEP could lead to hypoxemia and decreased respiratory compliance.
PROSPERO REGISTRATION:
CRD42022362379; registered October 09, 2022
Chromosome and nucleoli diversity in some species of Commelina L. (Commelinaceae) from Ethiopia
Authors: Gebrechristos Samuel , Kiros Gebrehiwet , Mulaw Guesh , Gebreabzgi Bahlibi 
Published: May 2023

Abstract
Background With about 100 species, Commelina is the largest genus of Commelinaceae in Africa. Although medicinal and economic benefits had been studied extensively, little is known about its cytological analysis. Hence, this study will focus on chromosome and nucleoli analysis of selected species of Commelina L. Somatic chromosomes were prepared from root tips that emerged from the nodes of stem cuttings that were made to stand submerged in water. The roots were pretreated in 8-hydroxyquinelin 3–5 hrs followed by fixation in 3:1 ethanol: acetic acid for 1–24 hrs at 4OC. Air-dry slides were prepared following cellulase and pectinase maceration at 37OC, the preparation was stained in Giemsa stain (PH 6.4), rinsed and mounted. Nucleoli were stained in silver nitrate solution.Results Chromosome numbers and Karyotype formula of the four species were found as C. africana 2n = 2x = 30 (12m + 10sm + 8st), C. benghalensis 2n = 6x = 66 (36m + 24sm + 6st), C. diffusa (Ginchi) 2n = 66 (28m + 26sm + 12st), C. diffusa (Jimma) 2n = 2x = 30 (10m + 8sm + 12st) and C. subulata 2n = 2x = 30 (18m + 10sm + 2st). According to Stebbins karyotype asymmetry, the karyotypes of C. africana and C. subulata were 2A type, while that of C. benghalensis and C. diffusa (Ginchi) were 2B type. 3A asymmetry type was obtained for C. diffusa (Jimma). Karotypes of Tradescantia were found to be monomodal for the Commelina species of the studied plant materials. Satellites were observed for species C. africana and C. diffusa with variation in number ranging from 2 to 6. The maximum number of nucleoli observed varies from two to four for Commelina.Conclusions This study reported karyotype and nucleoli of the Ethiopian Commelinaceae for the first time. The current investigation can be considered as an additional karyotype data to the earlier meiosis report for Ethiopian materials.
Integrative Transcriptomic Profiling Reveals Novel Biomarkers in Wilms Tumor
Authors: Avčin Simona Lucija , Črepinšek Klementina , Bizjan Barbara Jenko , Šket Robert , Kovač Jernej , Vrhovšek Blaž , Blazina Jerca , Blatnik Olga , Kordič Robert , Kitanovski Lidija , Jazbec Janez , Debeljak Maruša , Tesovnik Tine 
Published: May 2023

Abstract
Background: This study aimed to identify relevant transcriptomic universal biomarkers for Wilms tumor, the most common pediatric kidney cancer, independent of the histological type and stage.
Methods: Using next-generation sequencing, we analyzed the miRNA profiles of 74 kidney samples, which were divided into two independent groups: fresh frozen tissue and formalin-fixed paraffin-embedded tissue samples. Subsequent mRNA expression profiling and pathway analysis were performed to define the interplay and potential involvement of miRNAs and mRNA in Wilms tumor.
Results: Comparative analysis revealed 41 differentially expressed miRNAs, with 27 miRNAs having decreased expression and 14 miRNAs having increased expression in Wilms tumor tissue compared to healthy kidney tissue. Among global mRNA transcriptomic profile differences, cross-sectional analysis suggested only a limited list of genes potentially regulated by differentially expressed miRNAs in Wilms tumor.
Conclusions: Overall, our study is the first to determine the complete comprehensive miRNA and mRNA profiling of Wilms tumor using a multi-omics next-generation sequencing and bioinformatics approach, providing better insights into Wilms tumor pathogenesis. Identified universal Wilms tumor miRNAs have clear potential as biomarkers for the diagnosis and treatment of Wilms tumor, regardless of histological subtype and disease stage.
Design and Assessment of a Smartphone-based Educational Software for Malaria Detection
Authors: Alizadeh Ismaeil , Yousefi Saideh , Afshar Abbas Aghaei , Gidiglo Godwin Nutifafa , shirani-Bidabadi Leila , Gorouhi Mohammad Amin 
Published: May 2023

Abstract
Background Malaria is among the most important parasitic diseases transmitted by mosquitoes. It is an important health problem in many countries, especially tropical and subtropical countries. The aims of this study were to design an educational software for malaria and evaluate the satisfaction level of the software application among students of Vector Biology and Disease Control in Kerman University of Medical Sciences.Methods This descriptive cross-sectional study was conducted in the following two stages: 1. design and creation of educational software for Malaria and 2. Assessment of the level of satisfaction in the use of an electronic questionnaire among 75 students. Finally, the data were analyzed by SPSS software version 24.Results In the present study, educational software for malaria was designed and created. The results of the study showed that 53 (70.7%) of the participants were females and 22 (29.3%) of them were males. According to the results, 100% of users considered the software installation, size and working speed easy and appropriate. Totally, 93.3% of students stated that images in the software could convey the necessary content. Also, 100% of users found it easy to use the software and to communicate with the software developers. According to the results, more than 80% of students in this study preferred using this educational software to reading texts.Conclusion This educational software is recommended for use by the target community, experts in the healthcare sector and students in medical universities, especially students of Vector Biology and Control.
Direct syntheses of stereoisomers of 3-fluoro GABA and β-fluoroamine analogues of the calcium receptor (CaR) agonists, cinacalcet, tecalcet, fendilines and NPS R-467
Authors: Renault Yohann J. G. , Diao Jiayin , Cordes B B , Leach Katie , O'Hagan David 
Published: May 2023

Abstract
Synthetic routes following a sequential MacMillan organocatalytic asymmetric a-fluorination protocol for aldehydes and then reductive amination, has allowed ready access to bioactive b-fluoroamines. The approach is demonstrated with a short synthesis of (S)-3-fluoro-g-aminobutyric acid (3F-GABA) and was extended to b-fluoroamine stereoisomers of cinacalcet, tecalcet, fendilines and NPS R-467, all allosteric modulators of the calcium receptor (CaR). Stereoisomers of the fluorinated calcimimetic analogues were then assayed in a CaR receptor assay and a comparison of b-fluoroamine matched pair stereoisomers revealed a binding mode preference to the receptor as deduced from conformations which will be favoured as a consequence of the electrostatic gauche effect.
A study on the influences of high soil resistivity on the behavior of the grounding electrodes affected by lightning strokes
Authors: Gouda Osama E. , Dein Adel Z. El , Yassin Sara 
Published: May 2023

Abstract
This article presents a study for the investigation of the impacts of the high soil resistivity of homogeneous and heterogeneous soils on the behavior of the grounding electrodes affected by lightning strokes when the soil ionization phenomenon is considered. Investigations are done on the performance of the grounding electrode when the soil resistivity is taken between 500 and 2000 Ω.m in homogeneous soil and for positive and negative reflection factors in case of heterogeneous soils. In the proposed model of this study for heterogeneous soils, it is taken into account that each layer of soil has its own resistivity and its own permittivity which are frequency-dependent. The field of the soil ionization around grounding electrode under lightning is investigated. The results are presented to understand the performance of the grounding electrode such as impulse impedance, grounding potential rise, front time of lightning stroke, the peak current of a lightning stroke, and type of grounding electrode and effective length of a grounding electrode. The transmission line approach with the aid of the (ATP) which is defined as hybrid approach is used to compute the transient performance of the vertical and horizontal grounding electrodes under lightning influence. The results are supported by the latest published articles to ensure its credibility.
Cuproptosis-Related Risk Score Model Predicts Prognosis and Immune Characteristics of Esophageal Cancer
Authors: Li Peijin , Li Xuejiao , Shi Zehui , Guo Ziwei , Yang Cheng , Wu Qiyong 
Published: May 2023

Abstract
In cancer, the incidence of ESCA is the eighth and the mortality is the sixth worldwide. Copper is one of race metal elements in human body, which may play an important role in apoptosis in cancer. In recent years, Cuproptosis was proposed and studied as a less explored form of apoptosis. The main apoptotic mechanism is that copper binding to lipidated components, which are producted in the tricarboxylic acid cycle (TCA) directly, causes protein lipidation and subsequently leads to the lack of Fe-S cluster proteins, triggering proteotoxic stress and eventually apoptosis. Our study explored the role of cuproptosis in ESCA and built a risk model by using TCGA-ESCA date, named cuproptosis-related risk score model (CRRS). This model obtained cuproptosis-related risk scores to assess patient survival and prognosis. We also explored the differential expressed gene function enrichment, immune characteristics, and the tumor mutational load (TMB) between the two risk groups. Besides, we predicted the drug sensitivity of ESCA in the CRRS.
How do teenagers sleep? Analysis of factors related to sleep disorders in a group of Polish high school students
Authors: Tatar Dominika , Dębski Paweł , Bocian Bogusława , Bąkowska Małgorzata , Będkowska Joanna , Tropiejko Magda , Główczyński Patryk , Badura-Brzoza Karina 
Published: May 2023

Abstract
Insomnia is the most common sleep disorder in the adolescent population. Caused by genetic, biological, social and environmental factors, it affects disturbances in everyday functioning in many aspects of life. The aim of the study was to analyze the factors related to the occurrence of insomnia among high school students.
Material:
The study was conducted among 2364 high school students aged between 14 to 19 years old (the average age 17.92±1.10), of which there were 2068 girls and 295 boys.
Method: Athens Insomnia Scale (AIS) and authorial demographic data questionnaire involved questions about physical activity during the day, smoking, frequency of upper respiratory tract infections, problems with concentration and memory, school achievements, and the use of mobile devices at bedtime were used to assess examined parameters. The scale and questionnaires were shared on-line via social media.
Results: Analyzing the results obtained in the study group, the average score of 8.42±4.16 was obtained in the AIS, which allows the assessment of sleep disorders bordering on normal. After division into groups of girls and boys, 8.38±4.56 points were obtained, respectively for girls and 8.43±4.27pts. for boys - the difference was not statistically significant. Similarly, statistically significant differences were not found when dividing the groups into the one in which adolescents used the phone just before bedtime and the one that did not use it. The act of sleeping alone or with another person in the room also did not differentiate the groups statistically, as well as the place of residence. Statistically significant worse results in the AIS scale were obtained by people who declared worse physical activity during the day and smoking cigarettes, as well as those who reported more frequent problems with concentration and memory, had worse school performance and suffered from upper respiratory infections more often.
Conclusions
1. Sleep disorders may be related to factors such as smoking cigarettes or lack of physical activity, as well as difficulties in concentrating attention, memory disorders or worse academic performance and a tendency to contract upper respiratory tract infections.
2. Elimination of factors that may adversely affect the quality of sleep is particularly important in the group of adolescents, in whom the developing structures of the central nervous system may be particularly sensitive to deficiencies in this area
Use of Magnetotactic Bacteria as an MRI Contrast Agent for In Vivo Tracking of Adoptively Transferred Immune Cells
Authors: Nuschke Andrea , Sobey-Skelton Caitrin , Dawod Bassel , Kelly Brianna , Tremblay Marie-Laurence , Davis Christa , Rioux James A. , Brewer Kimberly 
Published: May 2023

Abstract
Purpose
In vivo immune cell tracking using MRI is a valuable tool for studying the mechanisms underlying successful cancer therapies. Current cell labeling methods using superparamagnetic iron oxide (SPIO) lack the specificity and persistence needed to track the fate and location of transplanted cells long-term. Magnetospirillium magneticum is a commercially available, iron-producing bacterium that can be taken up by, and live harmoniously within, mammalian cells as magneto-endosymbionts (MEs). MEs have shown promise as labeling agents for in vivo stem and cancer cell tracking but have yet to be evaluated in immune cells. This pilot study examined ME labeling in myeloid-derived suppressor cells (MDSCs), cytotoxic T lymphocytes (CTLs) and dendritic cells (DCs) and its effects on cell purity, function and MRI contrast.
Procedures:
MDSCs, CTLs and DCs were incubated with MEs at various ME labelling ratios (MLR) and various biological metrics and iron uptake were assessed. For in vivo imaging, MDSCs were labeled overnight with either MEs or SPIO (Molday ION Rhodamine B) and injected into C3 tumor-bearing mice via tail vein injection 24 days post-implant and scanned daily with MRI for one week to assess cellular quantification.
Results
Following incubations MDSCs contained 0.62 and 2.22 pg Fe/ cell. CTLs achieved Fe loading of < 0.5 pg/ cell and DCs achieved Fe loading of ~ 1.4pg/cell. The suppressive functionality of MDSCs at 1000MLR was not affected by ME labeling but was affected at 2000MLR. Markers of CTL dysfunction were not markedly affected by ME labeling, nor were DC markers. In vivo data demonstrated that the MDSCs labeled with MEs generated sufficient contrast to be detectable using TurboSPI, similar to SPIO-labeled cells.
Conclusions
Cells can be labeled with pre-clinically relevant amounts of MEs without compromising cell viability. Care must be taken at higher concentrations of MEs, which may affect the functional activity and/or morphology of some cell types. Immune cells with minimal phagocytic behaviour have much lower iron content per cells after incubation with MEs vs SPIO; however, MEs can successfully be used as a contrast agent for phagocytic immune cells.
Surgical outcomes in elective sigmoid resection for diverticulitis stratified according to indication: a propensity-score matched cohort study with 903 patients
Authors: Nocera Fabio , Haak Fabian , Posabella Alberto , Angehrn Fiorenzo Valente , Peterli Ralph , Müller Beat Peter , Steinemann Daniel C. 
Published: May 2023

Abstract
Purpose
Weighing the perioperative risk of elective sigmoidectomy is done regardless of the specific diverticulitis classification. The aim of this study is to evaluate surgical outcomes according to the classification grade and the indication.
Methods
All patients who underwent elective surgery for diverticulitis between 2011 and 2020 were included. They were divided into two groups: relative surgery indication (RSI) and absolute surgery indication (ASI). RSI included microabscess and recurrent uncomplicated disease. ASI included macroabscess and recurrent complicated disease. Propensity score-matching (PSM, 1:1) was performed.
Results
Criteria for RSI were fulfilled in 585npatients and for ASI in 318. After PSM, 318 RSI vs. 318 ASI patients were selected; baseline characteristics results were comparable. The proportion of planned laparoscopic resection was 93% in RSI versus 75% in ASI (p<0.001), and the conversion rate to open surgery for laparoscopic resection was 5.0% and 13.8% in RSI versus ASI (p<0.001). Major morbidity (Clavien/Dindo ≥IIIb) occurred less frequently in RSI (3.77% vs. 10%, p=0.003). A defunctioning stoma was formed in 0.9% and 11.0% in RSI vs ASI, respectively (p<0.001).
Conclusion
The lower risk for postoperative morbidity, the higher chance for a laparoscopic resection and the decreased rate of stoma formation are attributed to patients with recurrent uncomplicated diverticulitis or diverticulitis including a microabscess as compared to patients with complicated diverticulitis or diverticulitis and a macroabscess, and this applies even after PSM. Knowledge of stage-specific perioperative risk is important in determining indications and counseling patients.
Quantum Authentication Method based on Key-Controlled Maximally Mixed Quantum State Encryption
Authors: Lim Na-Hee , Choi Ji-Woong , Kang Min-Sung , Yang Hyung-Jin , Han Sang-Wook 
Published: May 2023

Abstract
Quantum authentication is a fundamental first step that ensures secure quantum communication. Although various quantum authentication methods have been proposed recently, their implementation efficiency is limited. This paper proposes a key-controlled maximally mixed quantum state encryption method using only a single qubit operation, which improves implementation feasibility and operation efficiency. We applied it to representative quantum authentication applications, namely, quantum identity and message authentication. The security of our authentication schemes was verified by analyzing the relationship between the integral ratio of Uhlmann's fidelity and probability of successful eavesdropping. Moreover, we demonstrate the higher authentication efficiency of the proposed scheme in a real quantum-channel noise environment.
Virological analysis of bronchoalveolar lavage fluid in patients with respiratory disease in Wuhan
Authors: Li Liangyu , Zhang Haiyue , Xiong Pei , Liu Chan , Wan Lu , Liu Mengling , Li Ruiyun , Shang Min , Liu Hailing , Ying Jing , Luo yuchuan , Wu Xiaojun , Chen Jianjun 
Published: May 2023

Abstract
Purpose
Identifying the cause of respiratory tract infections is important to reduce the burden of diagnosis and treatment. However, defining viral pneumonia based on viral detection in upper respiratory tract specimens gives ambiguous results. Thus, this study aimed to assess viral etiologies via bronchoalveolar lavage fluid (BALF) specimens from patients with different respiratory diseases.
Methods
BALF specimens (n = 335) from adult patients with respiratory disease were sampled between November 2020 and November 2021, and 22 respiratory viruses were detected using multiplex reverse-transcription polymerase chain reaction. Clinical data were collected from the hospital information system.
Results
At least one virus was identified in 118 (35.22%) cases. The most common viruses identified were influenza (33.13%), parainfluenza (3.28%), coronavirus (2.99%), rhinoviruses (2.09%), and respiratory syncytial (1.49%) viruses. No variations were observed in clinical symptoms between the viral and non-viral groups or among the different respiratory disease groups. The highest viral detection rate was observed in the tuberculosis group. Viral load was higher in co-infection than in single infection. In addition to influenza viruses, other viruses listed above are important in the etiology of lower respiratory tract infections in adults.
Conclusion
The findings suggest that rapid and accurate laboratory respiratory virus diagnosis is necessary for disease diagnosis and avoiding unnecessary antimicrobial drug use.
DNS and POD analysis of separated flow ina three-dimensional diffuser
Authors: Miró Arnau , Eiximeno Benet , Rodríguez Ivette , Lehmkuhl Oriol 
Published: May 2023

Abstract
A direct numerical simulation of a three dimensional diffuser at Reynoldsnumber Re = 10, 000 (based on inlet bulk velocity) has been per-formed using a low-dissipation finite element code. The geometry chosenfor this work is the Stanford diffuser, introduced by Cherry et al.(Int.J. Heat Fluid Fl. 29, 2008, pp. 803-811). Results have been exhaus-tively compared with the published data with a quite good agreement.A proper orthogonal decomposition and a dynamic mode decompositionanalysis of the main flow variables have been performed to identify themain characteristics of the large-scale motions. A combined motion of thelarge-scales has been found to originate in the top-right expansion cornerwith two clear features. A low-frequency diagonal cross-stream beatingfeature first reported by Malm et al. (J. Fluid Mech. 699, 2012, pp.320-351), has been clearly identified in the spatial modes of the stream-wise velocity components and the pressure, associated with the narrowband frequency of St ∈ [0.083, 0.01]. This feature has been found tobe more compacted near the expansion corner and elongates inside the diffuser section. A second low-frequency feature has been identified asso-ciated with the secondary flows and acting as a back and forth globalaccelerating-decelerating motion on the diffuser. The frequencies associ-ated to this motion are of St < 0.005, while the smallest observed inthis work has been St = 0.0013. This low-frequency motion observedin the Stanford diffuser point out the need for longer simulations.