bluuebunny/crossref_metadata_2025_split huggingface dataset:
Breast cancer epigenetics: comprehensive investigation of genes and chemical compounds involved in active DNA demethylation
Authors: Linowiecka Kinga , Guz Jolanta , Dziaman Tomasz , Domańska Olga Urbanowska - , Zarakowska Ewelina , Szpila Anna , Szpotan Justyna , Skalska-Bugała Aleksandra , Mijewski Paweł , Siomek-Górecka Agnieszka , Różalski Rafał , Gackowski Daniel , Oliński Ryszard , Foksiński Marek 
Published: April 2023

Abstract
Background: The experimental evidence has demonstrated that active DNA demethylation process, which involves TET proteins, can affect DNA methylation pattern. TET dependent demethylation residues in DNA hypomethylation by oxidation 5-methylcytosine (5-mC) to 5-hydroxymethylcytosine (5-hmC) and its derivatives. TETs’ activity may be upregulated by ascorbate. Given that aberrant DNA methylation of crucial genes implicated in breast carcinogenesis may be involved in tumor progression, we wanted to determine whether breast cancer patients exert changes in active DNA demethylation process. Moreover, we tried to verify if the expression level of genes involved in active DNA demethylation and products of this process may serve as predictive markers of breast cancer.
Methods: The study included blood samples from breast cancer patients (n=74) and healthy subjects (n=71). We applied qRT-PCR with Universal Probe Library probes for measurement of expression of genes involved in active demethylation process, and two dimensional ultra performance liquid chromatography with tandem mass spectrometry detection (2D-UPLC MS/MS) for 5–mC and its derivatives assessment. Ascorbate level was determined using UPLC-MS.
Results: Breast cancer patients exhibited significantly higher TET3 expression level, lower 5-mC and 5-hmC DNA levels. TET3expression was significantly increased in luminal B breast cancer patients with expression of hormone receptors. Moreover, ascorbate level in plasma of breast cancer patients was decreased with concomitant increase of sodium-dependent vitamin C transporters (SLC23A1 and SLC23A2) mRNA expression level.
Conclusions: Presented study indicate particular role of TET3 in DNA demethylation in breast carcinogenesis. Furthermore, correlation between SLC23A2 and 5-mC level, may provide new path for future breast cancer research.
Monitoring the response to Nintedanib treatment in a mouse model of lung fibrosis using longitudinal X-ray based lung function measurement
Authors: Khan Amara , Markus Andrea M. , Svetlove Angelika , Hülsmann Swen , Alves Frauke , Dullin Christian 
Published: April 2023

Abstract
Background: Idiopathic pulmonary fibrosis (IPF) is a chronic progressive, incurable, and debilitating fibrotic disease. Nintedanib – an oral tyrosine kinase inhibitor – significantly slows the IPF progression. However, reliable diagnostic tools for functional assessment of IPF at early stages are essentially lacking at both clinical and pre-clinical level.
Methods: In this study, we used a correlative set-up that combines X-ray based lung function (XLF) with microCT and whole body plethysmography (WBP) for a comprehensive functional and structural evaluation of lung fibrosis (LF) as well as for monitoring response to orally administered Nintedanib in the mouse model of bleomycin induced LF.
Results: The decline in lung function as early as one week after intratracheal bleomycin instillation was reliably detected by XLF, revealing the lowest decay rate in the LF mice compared to healthy ones. Simultaneously performed microCT and WBP measurements corroborated XLF findings by exhibiting reduced lung volume VinspµCT and tidal volume TVWBP at inspiration. In LF mice XLF also revealed profound improvement in lung function one week after Nintedanib treatment. This positive response to Nintedanib therapy was further substantiated by microCT and WBP measurements which also showed significantly improved VinspµCT and TVWBP in the Nintedanib treated mice.
Conclusion: By comparing the XLF data to structural features assessing the extent of fibrosis obtained by ex-vivo high-resolution synchrotron radiation-based imaging of lungs and classical histology we demonstrate that: i) a simple low dose x-ray measurement like XLF is sensitive enough to pick up treatment response, ii) Nintedanib treatment successfully improved lung function in a bleomycin induced LF mouse model and iii) the differences between the fully restored lung function and the partially reduced fiberotic burden compared to healthy and untreated mice. The presented analysis pipeline underlines the importance of a combined functional and anatomical readout to reliably measure treatment response and could easily be adapted to other preclinical lung disease models.
The Effect of Heart Rate Variability Biofeedback on Basketball Performance Tests
Authors: Göçmen Recep , Aktop Abdurrahman , Pınar Yeliz , Toktaş Neşe , Jandačková Vera Kristýna 
Published: April 2023

AbstractThe objective of the present study is to investigate the effects of ten-weeks heart rate variability biofeedback training on the basketball skill, free throw, and heart rate variability parameters. We aimed to increase vagal activation and to assess its effects on basketball performance and heart rate variability parameters.Twenty-fourbasketball players (experimental, n=12 and control, n=12) aged 18–24 volunteered to participate in this study. The experimental group participated in a ten-weeks heart rate variability biofeedback and basketball training program, while the control group participated only in a ten-weeks basketball training session. Basketball free-throw performance, basketball skills, and heart rate variability tests were applied to the experimental and control groups before and after ten-weeks of the intervention. We found that basketball free-throw performance, breathing frequency and heart rate variability parameters that reflect vagal modulation of parasympathetic activity improved in participants who underwent the ten-weeks heart rate variability biofeedback and basketball training, and not in those who underwent basketball training only. Finding suggest that heart rate variability biofeedback, along with basketball workouts, may contribute to better basketball free-throw performance potentially via improved autonomic nervous system functioning.
Molecular Aspect of Propofol's Effect on Cytotoxic and Long Non-Coding RNA (lncRNA) Expressions in MCF-7
Authors: AVCI Cigir BIRAY , EDGUNLU Tuba GOKDOGAN , SUZEK Tugba , OZATES Neslihan Pinar , BAGCA Bakiye GOKER , BILGIC Aysegul DEMIRTAS , OZDEMIR Cilem , UGUR Bakiye 
Published: April 2023

Abstract
Propofol (2,6-diisopropyl phenol) is an anesthetic agent frequently used in clinical practice. Besides its anesthetic properties, it exhibits many non-anesthetic effects, including antitumor activity. Propofol has an important effect on the spread of cancer, underlying molecular mechanisms of which are, however, not clearly known. In addition, Propofol may suppress the immune system by affecting both long non-coding RNAs (lncRNAs) and microRNAs (miRNAs) at the molecular level. In our study, we determined propofol's toxic effects, especially its effects on lncRNAs in the breast cancer cell line (MCF-7). Results of our study indicate that, when compared to untreated control cells, propofol administration decreases the expression of the lncRNAs which function as oncogenes (CCAT2, GACAT1, H19, PCAT1, SPRY4-IT1, UCA1) and increases the expression of lncRNAs which function as tumor suppressor genes (MEG3, TUSC7). Consequently, we suggest that propofol application on MCF-7 also supports the anti-cancer effect property by acting on lncRNAs.
Effect of Strategic Change and Adaptation on Organizational Performance A Comprehensive Literature Review
Authors: Ketemaw Adissu , Amente Chalchisa 
Published: April 2023

Abstract
Since they enable businesses to stay relevant and competitive in a dynamic business environment, strategic change and adaptation are crucial to organizational performance. This seminar review looks into how strategic change and adaptability affected organizational performance. Theoretical and empirical aspects of strategic change and adaptation are addressed in this paper. 28 articles that were systematically selected from various data base journals were reviewed using the compressive literature review method. According to the study's findings, anticipating strategic change and how to adapt it is essential for surviving in a fast-paced economic and technological environment. There are a number of factors that influnce strategic change and adaptation, but the external environment and external factors have the biggest impact on these two processes and ultimately on organizational performance. The findings of the study motivated the investigator to identify both theoretical and empirical implications. Also, a conceptual framework based on theoretical and empirical perspectives was developed with the aim to advance knowledge and build concepts in strategic change and adaptation.
Potential of silicon and carbon nanocages (C38, F-C38, Cl-C38, Si38, F-Si38, Cl-Si38) as anode materials in Li-ion battery and Mg-ion battery
Authors: Xu Lipeng , Tian Chongwang , Bao Chunjiang , Liu Tonggang , Xia Hengchao 
Published: April 2023

Abstract
The potential of various nanocages in metal-ion batteries are examined to propose novel materials with higher efficiency. The gap energy (EHLG), cohesive energy (Ecohesive) and adsorption energy (Eadsorption) of C38, F-C38, Cl-C38, Si38, F-Si38 and Cl-Si38 nanocages are calculated by theoretical methods. The interaction energy (Einteraction), cell voltage (Vcell) and theoretical capacity (Ctheory) of C38, F-C38, Cl-C38, Si38, F-Si38, Cl-Si38 nanocages in Li-ion batteries and Mg-ion batteries are calculated in gas phase and water. Results shown that the attaching of F and Cl can increase the Ecohesive and stability of carbon and silicon nanocages. The silicon nanocages in Mg-ion battery and Li-ion battery have higher Vcell and Ctheory than corresponding carbon nanocages. The Mg-ion batteries have higher Vcell and Ctheory values than Li-ion batteries. Results shown that F and Cl attached to silicon nanocages (F-Si38 and Cl-Si38) have the highest Vcell and Ctheory values in gas phase and water.
Improvement of Charcot-Marie-Tooth Phenotype with a Nanocomplex Treatment in Two Transgenic Models of CMT1A
Authors: Msheik Zeina , Masri Tarek El , Ndong-Ntoutoume Gautier MA , Vignaud Laetitia , Richard Laurence , Pinault Emilie , Faye Pierre-Antoine , Bregier Frédérique , Marquet Pierre , Favreau Frédéric , Vallat Jean-Michel , Sol Vincent , Sturtz Franck , Desmouliere Alexis , Massry Mohamed El 
Published: April 2023

Abstract
Curcumin was shown to exert beneficial effects on nerve function in peripheral neuropathies. Despite its prominent biological activities, curcumin presents with unfavorable pharmacokinetics. For this purpose, we have developed curcumin-loaded cyclodextrin/cellulose nanocrystals (NanoCur) to bypass this limitation. The current study aims to assess the potency of NanoCur in Charcot-Marie-Tooth disease type 1A (CMT1A) rodent models and compare its efficacy to Theracurmin® (Thera), a commercially available curcumin formulation, while elaborating on its mechanism of action. For that, a low dose of NanoCur was chronically administered for rodents and CMT1A neuropathology was assessed through a battery of functional, histological and biochemical tests. Toxicity and mechanism of action of NanoCur were evaluated both in-vivo & in-vitro. The overall study supports an improved motor function, associated with an amelioration in peripheral myelination in the NanoCur, but not Thera-treated CMT1A animals, combined to a high margin of safety. Furthermore, NanoCur appears to perform its effect through an alleviation of inflammatory pathways, involving macrophage recruitment to the diseased nerve. This study shows that NanoCur associates with therapeutic benefits at the cellular and functional levels in CMT1A with minimal systemic toxicity, promoting it as a potential therapeutic candidate for CMT1A disease and, possibly, other forms of neuropathy.
Latent Class Symptom Profiles of Colorectal Cancer Survivors with Cancer-Related Cognitive Impairment
Authors: Han Claire , Saligan Leorey , Crouch Adele , Kalady Matthew , Noonan Anne , Lee Lena , Von Ah Diane 
Published: April 2023

Abstract
Purpose. Colorectal cancer (CRC) survivors experience cancer-related cognitive impairment and co-occurring symptoms after cancer treatments. There has been little data to inform the risk factors of complex symptom phenotypes in CRC survivors.
Objectives.To determine if subgroups of CRC survivors after cancer treatments could be identified based on the cognitive impairment and common co-occurring symptoms (depression, anxiety, sleep disturbance, fatigue, and pain); and to explore risk factors (sociodemographic and clinical characteristics, perceived stress, and social support) of these subgroups.
Methods. Latent class profile analysis (LCPA) was used to identify subgroups based on self-reported symptoms in 64 CRC survivors. Cognitive impairment was measured by assessing subjective cognitive function using the Patient-Reported Outcome Measurement Information System (PROMIS) measure. The Kruskal-Wallis test and regression analyses were performed.
Results.Three distinct latent classes were identified (Class 1: All Low ‘28.1%’; Class 2: High Psychological Symptoms (depression/anxiety) ‘25%’; Class 3: High Somatic Symptoms (fatigue, sleep disturbance, and pain) with High Cognitive Impairment’46.9%’). Pain was the most distinguishable symptom across the latent classes. The high symptom burden group was associated with less time since cancer diagnosis, higher perceived stress levels, and poor emotional social support.
Conclusion.Our study adds to the information on interindividual variability in symptom experience of CRC survivors with cognitive impairment. Findings suggest a need for increased attention to screening for co-occurring symptoms (e.g., high pain) and future interventions focused on stress management and social supports.
Microfibrillar-associated Protein 4 as Potential Marker of Acute Relapse in Inflammatory Demyelinating Diseases of the Central Nervous System: Pathological and Clinical Aspects
Authors: Samadzadeh Sara , Olesen Mads Nikolaj , Wirenfeldt Martin , Möller Sören , Misu Tatsuro , Soelberg Kerstin , Frederiksen Jette Lautrup , Heegaard Steffen , Mariotto Sara , Fujihara Kazuo , Ruprecht Klemens , Andersen Thomas Levin , Marignier Romain , Lillevang Søren Thue , Flanagan Eoin P , Pittock Sean J , Kim Ho Jin , Bennett Jeffrey L , Paul Friedemann , Sorensen Grith Lykke , Weinshenker Brian G. , Lassmann Hans , Asgari Nasrin 
Published: April 2023

Abstract
Background and Objectives: Microfibrillar-associated protein 4 (MFAP4) is an extracellular matrix protein not previously described in the central nervous system (CNS). We aimed to determine MFAP4 CNS expression and measure cerebrospinal fluid (CSF) and serum levels.
Methods: Tissue was sampled at autopsy from patients with acute multiple sclerosis (MS) (n=3), progressive MS (n=3), neuromyelitis optica spectrum disorder (NMOSD) (n=2), controls (n=9), including 6 healthy controls (HC). MFAP4 levels were measured in 152 patients (49 MS, 62 NMOSD, 22 myelin oligodendrocyte glycoprotein associated disease (MOGAD), and 19 isolated optic neuritis (ION).
Results: MFAP4 localized to the meninges and the vascular/perivascular spaces, particularly intense in the optic nerve. At sites of active inflammation, MFAP4 reactivity was reduced in NMOSD and acute MS and to a lesser degree in progressive MS. CSF MFAP4 levels were reduced in patients during acute attacks compared to HC. There was a positive correlation between number of relapses and CSF MFAP4 levels (rho=0.33, p=0.004). CSF MFAP4 levels were lower in 53 samples obtained at presenting attack (mean U/mL: 14.3, MOGAD 9.7 and ION 14.6 relative to HC 17.9. (p=0.013, p=0.000 and p=0.019 respectively). All patients with acute ON relapse (n=68) had reduced CSF MFAP4 relative to HC (mean U/mL: 14.5 vs. 17.9, p=0.006). CSF MFAP4 levels correlated negatively with relapse severity (rho=-0.41, p=0.017).
Discussion.: MFAP4 immunoreactivity was observed in CNS and reduced at sites of active inflammation. CSF levels of MFAP4 were reduced following acute relapse and may serve as a marker of disease activity and attack severity.
Phenotypic, molecular, and symbiotic characterization of the rhizobial symbionts isolated from Acacia saligna grown in different regions in Morocco: An multivariate approach
Authors: LEBRAZI Sara , FADIL Mouhcine , CHRAIBI Marwa , BENBRAHIM Kawtar FIKRI 
Published: April 2023

Abstract
The introduced species Acacia saligna is a very promiscuous host as it can be efficiently nodulated with a wide range diversity of rhizobia taxa, including both fast and slow-growing strains. Fourteen nitrogen (N)-fixing bacteria were isolated from root nodules of wild Acacia saligna growing in distinct geographic locations in Morocco and were examined for their symbiotic efficiency and phenotypic properties. Multivariate tools, such as principal component analysis (PCA) and hierarchical clustering analysis (HCA), were used to study the correlation between phenotypic and symbiotic variables and discriminate and describe the similarities between different isolated bacteria with respect to all the phenotypic and symbiotic variables. Phenotypic characterization showed a variable response to extreme temperature, salinity and soil pH. At the plant level, the nodulation, nitrogen fixation, and the shoot and root dry weights were considered. The obtained results show that some of the tested isolates exhibit remarkable tolerances to the studied abiotic stresses while showing significant N2 fixation, indicating their usefulness as effective candidates for the inoculation of acacia trees. The PCA also allowed showing the isolates groups that present a similarity with evaluated phenotypic and symbiotic parameters. The genotypic identification of N2-fixing bacteria, carried out by the 16S rDNA approach, showed a variable genetic diversity among the 14 identified isolates, and their belonging to three different genera, namely Agrobacterium, Phyllobacterium and Rhizobium.
Cancelled total hip arthroplasty and knee arthroplasty: a retrospective analysis of 1003 patients
Authors: Cao Jian , Liao Kai , Mou Ping , Wang Wen-qi , Li Ze-xi , Zhou Zong-ke 
Published: April 2023

Abstract
Background The aim of the present study is to analyze the hospital length of stay (LOS), total hospital expense (THE), reasons, and subsequent fate of patients who had a total joint arthroplasty (TJA) cancelled.Methods In December 2020, we retrospectively reviewed a consecutive series of 18,508 patients who underwent primary total hip arthroplasty or knee arthroplasty between January 2009 and December 2018. Patients with unexpected cancellations of scheduled TJA surgeries were identified. LOS, THE, reasons for cancellations, and the number of patients who eventually performed arthroplasty were recorded.Results A total of 1,003 (5.4%) participants had scheduled TJA surgeries cancelled, which included 23 (2.3%) with two cancellations and 980 (97.7%) with one cancellation. The median LOS and THE of cancellations were 4 days (interquartile range [IQR] 3–7) and 4139 RMB (IQR 2611-6583.5), respectively. There were 720 patients (71.8%) who were cancelled due to medical-related reasons. Compared to non-medically related cancellation, medical-related cancellation had a higher age (60.9 vs 54.2, p < 0.001), LOS (5 vs 3, p < 0.001), and THE (4862 vs 2661, p < 0.001); meanwhile, the latter had a higher percentage of two cancellations (3.2% vs 0.0%, p = 0.002). During the follow-up, three hundred and twenty-three (32.3%) patients finally performed joint replacement in our institution, and the median time interval between the originally scheduled date of surgery and the actual date of surgery was 94 days (IQR 46-275.5).Conclusions This study suggests that the cancellation rate is relatively high in TJA practice, and medical-related cancellations are the most common. On the other hand, cancelling the operation will result in huge additional costs for patients and increased length of non-surgical hospital stay. Given that many of the reasons for cancellation are modifiable, a physician-guided pre-admission assessment is necessary to erase the concerns before the patient is admitted. Meanwhile, to prevent further delays, attention should be paid to patients whose TJA procedures have been cancelled and help them get their procedures rescheduled in a timely manner.
Multivalent nanobody-based sandwich enzyme-linked immunosorbent assay for sensitive detection of porcine reproductive and respiratory syndrome virus
Authors: Sun Mingxia , Sun Yue , Yang Yongbo , Zhao Man , Cao Dan , Zhang Minmin , Xia Dasong , Wang Tao , Gao Yanfei , Wang Shanghui , Wang Haiwei , Cai Xuehui , An Tongqing 
Published: April 2023

Abstract
Background The pandemic of the porcine reproductive and respiratory syndrome virus (PRRSV) has caused huge economic losses and continues to threaten the swine industry worldwide. Antibodies are critical for determining the sensitivity and specificity of diagnostic immunoassays. Recently, nanobodies have been increasingly used in diagnostic immunoassays because of their numerous advantages over traditional antibodies, including simple genetic engineering to improve the affinity and fusion with reported agents. This study is the first to develop a sandwich enzyme-linked immunosorbent assay (ELISA) with high sensitivity and specificity to detect the PRRSV.Results Thirteen nanobodies against PRRSV were prepared by using phage display technology and prokaryotically expressed. Two of those nanobodies with high affinity, Nb12 and Nb35, were selected and employed to develop the sandwich ELISA. To obtain greater sensitivity, a trivalent nanobody (3×Nb12) and a bivalent nanobody-HRP fusion protein (2×Nb35-HRP) were used as the capture antibody and the detecting antibody, respectively, in a subsequently modified sandwich ELISA. This modified ELISA was found to have high sensitivity for detecting PRRSV, with a detection limit of 10 TCID50/µL, which was approximately 200-fold greater than the single-copy nanobody-based sandwich ELISA. The developed assay shows high specificity and can detect almost all circulating lineages of PRRSV-2 in China.Conclusions The trivalent nanobodies and bivalent nanobody-HRP were applied to develop an improved sandwich ELISA for the first time, and the ELISA exhibits high sensitivity and specificity for detecting the target virus. This study provides suggestions for reforming nanobodies and for the further development of multivalent nanobody-based ELISAs for other various viruses.
Molecules with antimicrobial activity in the secretion of the arthrodial membrane gland of a harvester (Arachnida, Opiliones)
Authors: SILVA Norton Felipe dos Santos , WILLEMART Rodrigo Hirata , SILVA José Roberto Machado Cunha , Junior Pedro Ismael Silva 
Published: April 2023

Abstract

Because of the exoskeleton, arthropods must have flexible areas to be able to move. Such regions are called arthrodial membranes and are particularly vulnerable to bacteria and fungi. Here, we analyzed the secretion in the glands underneath it in a Neotropical harvester (Arachnida, Opiliones) and tested whether it has antiseptical properties. We punctured the membrane, collected and diluted the secretion and quantified proteins and peptides in a spectrophotometer. We also fractionated and analyzed the samples in reversed-phase high-performance liquid chromatography (RP-HPLC) and then incubated the treated fractions and determined growth inhibition by measuring absorbance. The secretions resulted in 42 fractions, among which two had activity against the Gram-positive bacteria Micrococcus luteus and against the yeast Candida albicans. The low concentrations at which the secretions were active are relevant from a biotechnological point of view. For the animals, the secretions possibly prevent infections, including when they are attacked in these regions by predators that pick that spot to bite.