bluuebunny/crossref_metadata_2025_split huggingface dataset:
Increased response to TPF chemotherapy promotes immune escape in hypopharyngeal squamous cell carcinoma
Authors: Li Ruichen , Yan Li , Tian Shu , Zhao Yang , Zhu Yi , Wang Xiaoshen 
Published: January 2023

Background: There is an urgent need to identify which patients would benefit from TPF chemotherapy in hypopharyngeal squamous cell carcinoma (HPSCC) and to explore new combinations to improve the treatment effect.Materials and methods: Gene-expression profiles in 15 TPF-sensitive patients were compared to 13 resistant patients. Immunohistochemistry (IHC) was performed to detect CD8+ T cells in 28 samples. Patient-Derived Tumor Xenograft (PDX) model and IHC were used to verify markers that optimize treatment for HPSCC.Results: Through RNA sequencing 188 genes were up-regulated in TPF chemotherapy-resistant (CR) tissues were involved in T cell activation, while 60 down-regulated genes were involved in glycolysis. Gene set enrichment analysis (GSEA) showed that chemotherapy-sensitive (CS) group upregulation of the pathways of glycolysis, while immune response was downregulated. CIBERSORT, MCP-counter, and IHC proved that most immune cells including CD8+ T cells in the CR significantly higher than that in CS group. Among the 16 up-regulated genes in CS had close associations, the most significant negative correlation between the gene level and CD8+ T cells existed in SEC61G. SEC61G was related to glycolysis, which was transcriptionally regulated by E2F1, and participated in antigen degradation through ubiquitin-dependent protein catabolic process. Palbociclib, combined with Cetuximab decreased the tumor burden and significantly suppressed the expression of E2F1 and SEC61G while activating MHC-I in PDX model.Conclusion: Enhanced glycolysis promoted immune escape, but increased response to TPF chemotherapy. SEC61G was the center of the molecular network and targeting the E2F1/SEC61G pathway increased the expression level of MHC-I.
Neutralization of excessive CCL28 improves wound healing in diabetic mice
Authors: Chen Zhenlong , Haus Jacob M. , DiPietro Luisa A. , Koh Timothy J. , Minshall Richard D. 
Published: January 2023

Introduction: Chronic, non-healing skin wounds such as diabetic foot ulcers (DFUs) are common in patients with type 2 diabetes mellitus (T2DM) and often result in limb amputation and even death. However, mechanisms by which T2DM and inflammation negatively impact skin wound healing remains poorly understood. Here we investigate a mechanism by which an excessive level of chemokine CCL28, through its receptor CCR10, impairs wound healing in patients and mice with T2DM.Methods &amp; Results: Firstly, a higher level of CCL28 was observed in skin and plasma in both patients with T2DM, and in obesity-induced type 2 diabetic db/db mice. Compared with WT mice, adipose tissue from db/db mice released 50% more CCL28, as well as 2- to 3-fold more IL-1β, IL-6, and TNF-α, and less VEGF, as determined by ELISA measurements. Secondly, overexpression of CCL28 with adenovirus (Adv-CCL28) caused elevation of proinflammatory cytokines as well as CCR10 expression and also reduced eNOS expression in the dorsal skin of WT mice as compared with control Adv. Thirdly, topical application of neutralizing anti-CCL28 Ab dose-dependently accelerated wound closure and eNOS expression, and decreased IL-6 level, with an optimal dose of 1 μg/wound. In addition, mRNA levels of eNOS and anti-inflammatory cytokine IL-4 were increased as shown by real-time RT-PCR. The interaction between eNOS and CCR10 was significantly reduced in diabetic mouse wounds following application of the optimal dose of anti-CCL28 Ab, and eNOS expression increased. Finally, enhanced VEGF production and increased subdermal vessel density as indicated by CD31 immunostaining were also observed with anti-CCL28 Ab.Discussion: Taken together, topical application of neutralizing anti-CCL28 Ab improved dorsal skin wound healing by reducing CCR10 activation and inflammation in part by preventing eNOS downregulation, increasing VEGF production, and restoring angiogenesis. These results indicate anti-CCL28 Ab has significant potential as a therapeutic strategy for treatment of chronic non-healing diabetic skin wounds such as DFUs.
Living myocardial slices: Advancing arrhythmia research
Authors: Amesz Jorik H. , Zhang Lu , Everts Bian R. , De Groot Natasja M. S. , Taverne Yannick J. H. J. 
Published: January 2023

Living myocardial slices (LMS) are ultrathin (150–400 µm) sections of intact myocardium that can be used as a comprehensive model for cardiac arrhythmia research. The recent introduction of biomimetic electromechanical cultivation chambers enables long-term cultivation and easy control of living myocardial slices culture conditions. The aim of this review is to present the potential of this biomimetic interface using living myocardial slices in electrophysiological studies outlining advantages, disadvantages and future perspectives of the model. Furthermore, different electrophysiological techniques and their application on living myocardial slices will be discussed. The developments of living myocardial slices in electrophysiology research will hopefully lead to future breakthroughs in the understanding of cardiac arrhythmia mechanisms and the development of novel therapeutic options.
Selenium seed priming enhanced the growth of salt-stressed Brassica rapa L. through improving plant nutrition and the antioxidant system
Authors: Hussain Saber , Ahmed Shakil , Akram Waheed , Li Guihua , Yasin Nasim Ahmad 
Published: January 2023

Various abiotic stresses may affect the germination, growth, and yield of direct-seeded vegetable crops. Seed priming with effective antioxidant mediators may alleviate these environmental stresses by maintaining uniformity in seed germination and improving the subsequent health of developing seedlings. Salt-induced stress has become a limiting factor for the successful cultivation of Brassica rapa L., especially in Southeast Asian countries. The present study was performed to elucidate the efficacy of seed priming using selenium (Se) in mitigating salt-induced oxidative stress in turnip crops by reducing the uptake of Na+. In this study, we administered three different levels of Se (Se-1, 75 μmol L−1; Se-2, 100 μmol L−1; and Se-3, 125 μmol L−1) alone or in combination with NaCl (200 mM). Conspicuously, salinity and Se-2 modulated the expression levels of the antioxidant genes, including catalase (CAT), peroxidase (POD), superoxide dismutase (SOD), and ascorbate peroxidase (APX). The upregulated expression of stress-responsive genes alleviated salt stress by scavenging the higher reactive oxygen species (ROS) level. The stress ameliorative potential of Se (Se-2 = 100 μmol L−1) enhanced the final seed germination percentage, photosynthetic content, and seedling biomass production up to 48%, 56%, and 51%, respectively, under stress. The advantageous effects of Se were attributed to the alleviation of salinity stress through the reduction of the levels of malondialdehyde (MDA), proline, and H2O2. Generally, treatment with Se-2 (100 μmo L−1) was more effective in enhancing the growth attributes of B. rapa compared to Se-1 (75 μmo L−1) and Se-3 (125 μmo L−1) under salt-stressed and non-stressed conditions. The findings of the current study advocate the application of the Se seed priming technique as an economical and eco-friendly approach for salt stress mitigation in crops grown under saline conditions.
Come together: The importance of arts and cultural engagement within the Liverpool City Region throughout the COVID-19 lockdown periods
Authors: Chapple Melissa , Anisimovich Antonina , Worsley Joanne , Watkins Megan , Billington Josie , Balabanova Ekaterina 
Published: January 2023

IntroductionArts and cultural engagement activities have long been found to support wellbeing within the general population. In particular, community arts and cultural involvement during the COVID-19 pandemic have been an invaluable source of mental health and wellbeing support for many individuals across the globe. The initial move to remote engagement following the first United Kingdom lockdown demonstrated the importance of hybrid provisions, with isolated and vulnerable individuals finding online provisions important for wellbeing. With restrictions on movement and service access in the United Kingdom having gradually eased from March 2021, it is now important to explore how individuals navigated the ability to engage with either remote or in-person provisions. The current study aimed to explore the impact of the COVID-19 pandemic on arts and cultural engagement during periods of restrictions and initial easings on movement within the Liverpool City Region.MethodThe study consisted of two waves of qualitative interviews within a broader longitudinal study. Twelve interviews were conducted during wave 1, which aimed to capture data during the initial COVID-19 lockdown period and the initial easing of restrictions. Eight of these participants were interviewed again for wave 2, which aimed to capture data during the winter 2020 lockdown period.ResultsFramework analysis revealed three overarching themes: (1) The Importance of Arts and Culture for Personal Enrichment, (2) Belongingness through Socialization, and (3) Transitioning and Adjusting Throughout the COVID-19 Pandemic.DiscussionFindings presented in the current study provide further evidence of the value of arts and cultural activities in supporting wellbeing. Specifically, the current data emphasize the value of arts and cultural engagement throughout the COVID-19 pandemic and particularly during times of national restriction. Furthermore, the current study demonstrated that remote engagement provided important wellbeing support throughout the pandemic in a way that protected against mental health consequences, but with limitations on feelings of social connectedness within online environments. Amidst continuing risks from the COVID-19 virus and feelings of uncertainty, this study highlights the importance of hybrid provisions.
The association between leisure activity patterns and the prevalence of mild cognitive impairment in community-dwelling older adults
Authors: Zhang Yuanyuan , Yang Xinxin , Guo Linlin , Xu Xinyi , Chen Bingyu , Ma Xiaowei , Li Yan 
Published: January 2023

ObjectivesThe study aimed to identify leisure activity patterns among older adults and explore the differences in the prevalence of mild cognitive impairment (MCI) of these patterns.MethodsA total of 352 older adults aged ≥ 65 years in Shijiazhuang were enrolled in this study from June to September 2021. Their leisure activities and cognition were evaluated. Latent class analysis and logistic regression models were used for analysis. The manuscript was organized according to the STROBE guidelines.ResultsWe identified three latent classes of leisure activities: multidomain activities (MDA) class (55%), self-improvement activities (SIA) class (21%), and family-centered activities (FCA) class (24%). Three latent classes significantly differed in general characteristics (gender, education, and body mass index) and the prevalence of MCI. The prevalence of MCI was 3.694 times higher in older adults in the FCA class than in older adults in the MDA class (OR = 3.694, P &lt; 0.001) and 2.573 times higher in older adults in the SIA class (OR = 2.573, P = 0.036).ConclusionPatterns of low participation in intellectual activities were associated with MCI. Identifying the heterogeneity in leisure activity engagement in later life was critical for tailoring interventions and designing programs that can improve the cognitive function of older adults.
Emotional stimulation processing characteristics in depression: Meta-analysis of eye tracking findings
Authors: Huang Genying , Li Yafang , Zhu Huizhong , Feng Hong , Shen Xunbing , Chen Zhencai 
Published: January 2023

ObjectiveTo systematically evaluate the attentional bias in patients with depression toward emotional stimuli and to explore eye movement indicators and potential regulatory variables that can distinguish such patients from healthy individuals.MethodsCase–control studies regarding eye-tracking in major depressive disorder published in PubMed, Web of Science, ScienceDirect, The Cochrane Library, EBSCOhost, Embase, China National Knowledge Infrastructure, Wanfang, and VIP databases from database initiation until March 12, 2022 were included in the present meta-analysis. Two researchers independently screened the literature and performed data extraction. The quality of the literature was assessed using the Newcastle–Ottawa quality assessment scale.RevMan 5.4 software was used for Meta-analysis.ResultsOverall, 14 studies were included, including 1,167 participants (Ndepression = 474; Nhealthy = 693). We found that (1) fixation duration was significantly lower for positive emotional stimuli in the depression group than that in the healthy group; however, for negative stimuli, the fixation duration was significantly more in the depression group than in the healthy group. No significant difference was observed in terms of neutral emotional stimuli between groups. (2) Patients with depression exhibited a significantly lower fixation count for positive emotional stimuli than healthy individuals, whereas the fixation count for negative emotional stimuli was significantly higher in the depression group than in the healthy group. No significant difference was found for neutral emotional stimuli between groups. (3) No significant difference was detected in terms of the first fixation duration of the positive, negative, and neutral emotional stimuli between groups. (4) subgroup analysis indicated that age effected fixation duration for positive emotional stimuli. In addition, age and the type of negative emotional picture (sad, dysphoric, threat, anger) effected fixation duration for negative emotional stimuli.ConclusionOur research supports that patients with depression exhibit a negative attention bias toward emotional stimuli, and the fixation duration and fixation counts may be used as auxiliary objective indicators for depression screening.
Life after lockdown: The experiences of older adults in a contactless digital world
Authors: Morrison Benjamin A. , Nicholson James , Wood Becca , Briggs Pam 
Published: January 2023

IntroductionThe digital response to the Coronavirus (COVID-19) pandemic and its effects on the lives of older adults has been well-documented, but less is known about how they experienced the post-lockdown re-emergence into a relatively contactless digital society.MethodsWe report the findings from a qualitative survey (n = 93) and subsequent interviews (n = 9) with older adults aged 50+, where they describe their struggles with some of the newly implemented digital interactions. These struggles cover a range of settings but include using contactless payments, QR codes and apps to facilitate transactions in cafes, bars, and restaurants.ResultsA thematic analysis of our data revealed the intrinsic (e.g. digital literacy) and extrinsic (e.g. malfunctioning technology) factors that limited social inclusion for these participants, and that sometimes even led to moments of public humiliation.DiscussionOur findings shed light on some of the motivational factors that underpin the age-related digital divide, whilst also highlighting the role of self-directed agism in limiting motivations to learn new digital routines.
Mediating roles of perceived social support and sense of security in the relationship between negative life events and life satisfaction among left-behind children: A cross-sectional study
Authors: Liu Na , Li Xinzheng , Ding Xuemei , Liu Haixia , Zhang Xiaoli 
Published: January 2023

ObjectiveLife satisfaction is closely related to the quality of life. Previous studies showed that left-behind children have low life satisfaction levels due to their separation from their parents. Therefore, it is significant to explore the risk factors and protective factors of left-behind children’s life satisfaction to improve their life quality. Therefore, the purpose of this study was to determine the mediating roles of perceived social support and sense of security in the relationship between negative life events and life satisfaction among left-behind children.MethodsA survey was conducted on 281 left-behind children in rural Shandong, China using negative life events, perceived social support, a sense of security, and life satisfaction scales.ResultsNegative life events had a negative impact on life satisfaction (β = −0.34, p = 0.001). In addition, not only does social support mediate between negative life events and life satisfaction (β = −0.21, p = 0.001), but also in between, security (β = −0.05, p = 0.030). Moreover, social support and security act as a chain intermediary between negative life events and life satisfaction (β = −0.03, p = 0.010), with an indirect effect share of 4.76%.ConclusionNegative life events could directly or indirectly affect the life satisfaction of left-behind children through the chain-mediating effects of perceived social support or the sense of security alone. Perceived social support and the sense of security are two important targets for further improving the life satisfaction of LBC.
Projecting the chronic disease burden among the adult population in the United States using a multi-state population model
Authors: Ansah John P. , Chiu Chi-Tsun 
Published: January 2023

IntroductionAs the United States population ages, the adult population with chronic diseases is expected to increase. Exploring credible, evidence-based projections of the future burden of chronic diseases is fundamental to understanding the likely impact of established and emerging interventions on the incidence and prevalence of chronic disease. Projections of chronic disease often involve cross-sectional data that fails to account for the transition of individuals across different health states. Thus, this research aims to address this gap by projecting the number of adult Americans with chronic disease based on empirically estimated age, gender, and race-specific transition rates across predetermined health states.MethodsWe developed a multi-state population model that disaggregates the adult population in the United States into three health states, i.e., (a) healthy, (b) one chronic condition, and (c) multimorbidity. Data from the 1998 to 2018 Health and Retirement Study was used to estimate age, gender, and race-specific transition rates across the three health states, as input to the multi-state population model to project future chronic disease burden.ResultsThe number of people in the United States aged 50 years and older will increase by 61.11% from 137.25 million in 2020 to 221.13 million in 2050. Of the population 50 years and older, the number with at least one chronic disease is estimated to increase by 99.5% from 71.522 million in 2020 to 142.66 million by 2050. At the same time, those with multimorbidity are projected to increase 91.16% from 7.8304 million in 2020 to 14.968 million in 2050. By race by 2050, 64.6% of non-Hispanic whites will likely have one or more chronic conditions, while for non-Hispanic black, 61.47%, and Hispanic and other races 64.5%.ConclusionThe evidence-based projections provide the foundation for policymakers to explore the impact of interventions on targeted population groups and plan for the health workforce required to provide adequate care for current and future individuals with chronic diseases.
Analysis of network public opinion on COVID-19 epidemic based on the WSR theory
Authors: Yang Kun , Zhu Junqi , Yang Li , Lin Yu , Huang Xin , Li YunPeng 
Published: January 2023

ObjectiveTo obtain the influencing factors of public opinion reactions and to construct a basic framework of the factors causing the occurrence of online public opinion in the epidemic area.MethodsThe hot news comments on microblogs during the epidemic in Shanghai were collected and analyzed with qualitative analysis, grounded theory, and the “Wuli-Shili-Renli” (WSR) methodology as an auxiliary method.Results(1) Three core categories of the Wuli system, the Shili system, and the Renli system, 15 main categories, and 86 categories that influence the development of network public opinion are obtained. (2) WSR Elements Framework Of Network Public Opinion (WSR-EFONPO) is established. (3) The WSR-EFONPO is explained.ConclusionThe framework of factors for the occurrence of network public opinion is proposed, and the development process of network public opinion under COVID-19 is sorted out, which is of great theoretical value in guiding the public in the epidemic area to form reasonable behavior.
The multicomponent residue depletion of Gelsemium elegans in pig tissues, urine, and plasma
Authors: Wu Yong , Long Xue-Ming , Liu Gao-Feng , Bai Xia , Sun Zhi-Liang , Liu Zhao-Ying 
Published: January 2023

IntroductionGelsemium elegans (G. elegans) as a traditional medicinal plant used in livestock production. The use of G. elegans in veterinary clinics may pose safety risks to human health.ObjectivesThe aim of this study was to investigate tissue residue depletion in pigs fed G. elegans powder.MethodsA precise quantitation method and a simultaneous semi-quantitation method for multiple components independently of standards in pig tissues were developed for the first time. The two methods were validated in terms of specificity, LODs, LOQs, linearity, accuracy, precision, and matrix effects. They were then applied to a tissue residue depletion study after G. elegans powder at a dose of 2% per kg feed were fed to pigs.ResultsCompared with precise quantitation, the method validation results indicated that the semi-quantitation method was reliable and acceptable for multicomponent quantification independent of standards. Many G. elegans alkaloids are widely distributed in most tissues of pigs. Tissue residue depletion studies indicated that 14-hydroxygelsenicine, 11-hydroxygelsenicine, and gelsemoxonine could be used as potential residue markers, and pancreas, small intestine, and lung tissues could be considered as potential residue target tissues of G. elegans. In addition, both urine and plasma could be used to predict 14-hydroxygelsenicine and gelsemoxonine residues in the liver, pancreas, and small intestinal tissues of pigs.ConclusionThe developed semi-quantification method can be applied to monitor the application and residue of G. elegans. The results provide scientific evidence for evaluating the safety of animal-derived food from G. elegans for consumers and will be helpful for its application and future development.
EFL Students’ Anxiety in Oral Presentation in Thesis Examination during Covid- 19 Pandemic Era: Factors and Strategies
Authors: Nur Sahril , Sakkir Geminastiti , Rosmini  
Published: January 2023

This research discusses EFL students’ anxiety in oral presentation in thesis examinations. This research aims to reveal the factors causing students’ anxiety and the strategy that EFL students apply to overcome/reducing their anxiety. The researchers conducted this research in Islamic Higher Education and this research used a qualitative method with a case study approach. The participants of this research were 7 (seven) EFL students that the data were analyzed using thematic analysis based on Braun and Clarke (2016). Then, the researchers used Wilson’s (2004) model semi-structured interview that the researchers arranged as the research instruments. The results revealed that the factors causing students’ anxiety generally: linguistic factors and non-linguistic factors (psychology factors, environment factors, and individual factors). Furthermore, the researchers found that EFL students apply strategies to overcome anxiety in oral presentation: preparation, relaxation, positive thinking, peer seeking, self-entertaining, and praying. Therefore, the lecturers have to know their students’ anxiety in oral presentation and they have to apply the best strategy in teaching English as Foreign Language.