bluuebunny/crossref_metadata_2025_split huggingface dataset:
Hypoxia mitigation by manganese-doped carbon dots for synergistic photodynamic therapy of oral squamous cell carcinoma
Authors: Zhang Zhe , Xu Yongzhi , Zhu Tingting , Sang Zhiqin , Guo Xiaoli , Sun Yu , Hao Yuanping , Wang Wanchun 
Published: April 2023

Photodynamic therapy (PDT) is widely used for cancer treatment due to its non-invasive and precise effectiveness, however, hypoxia in the tumor microenvironment greatly limits the efficacy of photodynamic therapy. Compared with conventional photosensitizers, carbon dots (CDs) have great potential. Therefore, developing a water-soluble, low-toxicity photosensitizer based on CDs is particularly important, especially one that can enhance the photodynamic efficacy using the tumor microenvironment to produce oxygen. Herein, manganese-doped carbon dot (Mn-CDs, ∼2.7 nm) nanoenzymes with excellent biocompatibility were prepared by a solvothermal method using ethylenediaminetetraacetic acid manganese disodium salt hydrate and o-phenylenediamine as precursors. TEM, AFM, HR-TEM, XRD, XPS, FT-IR, ζ potential, DLS, UV-Vis, and PL spectra were used to characterize the Mn-CDs. Cancer resistance was assessed using the CCK-8 kit, calcein AM versus propidium iodide (PI) kit, and the Annexin V-FITC/PI cell apoptosis assay kit. The obtained Mn-CDs have excellent near-infrared emission properties, stability, and efficient 1O2 generation. Notably, the manganese doping renders CDs with catalase (CAT)-like activity, which leads to the decomposition of acidic H2O2in situ to generate O2, enhancing the PDT efficacy against OSCC-9 cells under 635 nm (300 mW·cm−2) irradiation. Thus, this work provides a simple and feasible method for the development of water-soluble photosensitizers with oxygen production, presenting good biosafety for PDT in hypoxic tumors.
A circular bioprocess application of algal-based substrate for Bacillus subtilis natto production of γ-PGA
Authors: Parati Mattia , Philip Catherine , Mendrek Barbara , Townrow David , Khalil Ibrahim , Tchuenbou-Magaia Fideline , Stanley Michele , Kowalczuk Marek , Adamus Grazyna , Radecka Iza 
Published: April 2023

Poly-γ-glutamic acid (γ-PGA) is a bio-derived water-soluble, edible, hydrating, non-immunogenic polymer. Bacillus subtilis natto is a wild-type γ-PGA producer originally isolated from Japanese fermented natto beans whose activity has been shown to be enhanced through ion-specific activation of Extrachromosomal DNA maintenance mechanisms. Being a GRAS γ-PGA producer, this microorganism has attracted great interest in its use within an industrial context. Here we successfully synthesised amorphous, crystalline and semi-crystalline γ-PGA between 11–27 g/L. In line with circular economy principles, scalable macroalgal biomass has been evaluated as substrate for γ-PGA, displaying great potential in both yields and material composition. In this study whole cell, freeze dried seaweed -namely Laminaria digitata, Saccharina latissima and Alaria esculenta-were pre-treated by means of mechanical methods, sterilised and subsequently inoculated with B. subtilis natto. High shear mixing was found to be the most suitable pre-treatment technique. Supplemented L. digitata (9.1 g/L), S. latissima (10.2 g/L), A. esculenta (13 g/L) displayed γ-PGA yields comparable to those of standard GS media (14.4 g/L). Greatest yields of pure γ-PGA were obtained during the month of June for L. digitata (Avg. 4.76 g/L) comparable to those obtained with GS media (7.0 g/L). Further, pre-treated S. latissima and L. digitata complex media enabled for high molar mass (4,500 kDa) γ-PGA biosynthesis at 8.6 and 8.7 g/L respectively. Compared to standard GS media, algal derived γ-PGA displayed significantly higher molar masses. Further studies will be necessary to further evaluate the impact of varying ash contents upon the stereochemical properties and modify the properties of algal media based γ-PGA with the aid of key nutrients; however, the material synthesised to date can directly displace a number of fossil fuel derived chemicals in drug delivery applications, cosmetics, bioremediation, wastewater treatment, flocculation and as cryoprotectants.
Active faulting of the Nanhe Fault and relation to the Anninghe Fault zone in the late Quaternary, eastern Tibetan Plateau
Authors: Ma Chao , Liu Yufa , An Like , Yang Xing , Liu Shao , Zhang Wei 
Published: April 2023

Faults along the boundaries of active tectonic blocks are the main structures that are responsible for major earthquakes in mainland China. Investigating the geometric distribution, rupture behavior, and paleoseismic history of these faults is the prerequisite for constraining geodynamic models and regional seismic hazard analyses. The Nanhe Fault, located at the eastern boundary of the Sichuan–Yunnan Block near Mianning County, has been paid less attention so far due to insufficient historical records of major earthquakes. In this paper, we focused on the Nanhe Fault and conducted satellite imagery interpretation, field investigations, and trench excavations. Our findings indicate that the Nanhe Fault initiates north of Mianning County; the north segment of the fault is connected with the Anninghe Fault; and it extends for about 70 km south-westward and terminates southwest of Ermaga Village. The fault has been faulting in the late Late Pleistocene with a left-lateral strike-slip rate of 2.40–2.56 mm/yr, while in the late Holocene, the left-lateral strike-slip and vertical slip rates are 2.50–2.60 mm/yr and about 0.60 mm/yr, respectively. Three paleoseismic events (5373–4525 BC, AD 1193–1576, and AD 1496–1843) were identified by excavating trenches at the Nanhe Fault. A comparative analysis of paleoseismic events between the Nanhe and the Anninghe fault indicates that both faults may have induced cascade rupture or triggered earthquakes—such related events may have occurred in 1496–1627. Additionally, by comparing the kinematic relationship of the faults at the eastern boundary of the Sichuan–Yunnan Block, we propose that the Nanhe Fault takes part in strain partitioning along the boundary. This interpretation reasonably explains the loss of the sliding rate between the Anninghe and Zemuhe faults, which also supports the GPS inversion results, and the discontinuous deformation model for the eastern margin of the Tibetan Plateau.
ECSIT is essential for RANKL-induced stimulation of mitochondria in osteoclasts and a target for the anti-osteoclastogenic effects of estrogens
Authors: Marques-Carvalho Adriana , Sardão Vilma A. , Kim Ha-Neui , Almeida Maria 
Published: April 2023

IntroductionEstrogens inhibit bone resorption and preserve bone mass, at least in part, via direct effects on osteoclasts. The binding of RANKL, the critical cytokine for osteoclast differentiation, to its receptor in osteoclast precursor cells of the monocyte lineage recruits the adaptor protein TRAF6 and activates multiple signaling pathways. Early effects of RANKL include stimulation of mitochondria. 17β-estradiol (E2) prevents the effects of RANKL on mitochondria and promotes mitochondria mediated apoptotic cell death. However, the molecular mechanisms responsible for the actions of RANKL and estrogens on mitochondria remain unknown. Evolutionarily Conserved Signaling Intermediate in Toll Pathway (ECSIT) is a complex I-associated protein that regulates immune responses in macrophages following the engagement of Toll-like receptors, which also recruit TRAF6. Here, we examined whether ECSIT could be implicated in the rapid effects of RANKL and E2 on osteoclast progenitors.MethodsBone marrow-derived macrophages (BMMs) from C57BL/6 mice were cultured with RANKL (30 ng/ml) with or without E2 (10-8 M). ECSIT-TRAF6 interaction was evaluated by co-immunoprecipitation and ECSIT levels in mitochondria and cytosolic fractions by Western blot. ShRNA lentivirus particles were used to knockdown ECSIT. Osteoclasts were enumerated after tartrate-resistant acid phosphatase staining. Oxygen consumption and extracellular acidification rates were measured with Seahorse XFe96 Analyzer. ATP, lactate, and NAD/NADH were measured with commercial assay kits. NADH oxidation to NAD was used to evaluate Complex I activity. Total and mitochondrial ROS, and mitochondrial membrane potential were measured with H2DCFDA, MitoSOX, and TMRM probes, respectively. Degradation of DEVD-AFC was used to measure Caspase-3 activity.ResultsWe found that RANKL promoted ECSIT-TRAF6 interaction and increased the levels of ECSIT in mitochondria. E2 abrogated these effects of RANKL. Silencing of ECSIT decreased osteoclast differentiation and abrogated the inhibitory effects of E2 on osteoclastogenesis. Loss of ECSIT decreased complex I activity, oxygen consumption, NAD+/NADH redox ratio, and ATP production and increased mitochondrial ROS. In the absence of ECSIT, the stimulatory actions of RANKL on complex I activity and all other markers of oxidative phosphorylation, as well as their inhibition by E2, were prevented. Instead, RANKL stimulated apoptosis of osteoclast progenitors.DiscussionThese findings suggest that dysregulated mitochondria cause a switch in RANKL signaling from pro-survival to pro-apoptotic. In addition, our results indicate that ECSIT represents a central node for the early effects of RANKL on mitochondria and that inhibition of ECSIT-mediated mitochondria stimulation might contribute to the bone protective actions of estrogens.
USP10 is a potential mediator for vagus nerve stimulation to alleviate neuroinflammation in ischaemic stroke by inhibiting NF-κB signalling pathway
Authors: Xie Chenchen , Gao Xiang , Liu Gang , Tang Hao , Li Changqing 
Published: April 2023

BackgroundVagus nerve stimulation (VNS) has a protective effect on neurological recovery in ischaemic stroke. However, its underlying mechanism remains to be clarified. Ubiquitin-specific protease 10 (USP10), a member of the ubiquitin-specific protease family, has been shown to inhibit the activation of the NF-κB signalling pathway. Therefore, this study investigated whether USP10 plays a key role in the protective effect of VNS against ischemic stroke and explore its mechanism.MethodsIschaemic stroke model was constructed by transient middle cerebral artery occlusion (tMCAO) in mice. VNS was performed at 30 min, 24hr, and 48hr after the establishment of tMCAO model. USP10 expression induced by VNS after tMCAO was measured. LV-shUSP10 was used to establish the model with low expression of USP10 by stereotaxic injection technique. The effects of VNS with or without USP10 silencing on neurological deficits, cerebral infarct volume, NF-κB pathway activation, glial cell activation, and release of pro-inflammation cytokines were assessed.ResultsVNS enhanced the expression of USP10 following tMCAO. VNS ameliorated neurological deficits and reduced cerebral infarct volume, but this effect was inhibited by silencing of USP10. Activation of the NF-κB pathway and the expression of inflammatory cytokines induced by tMCAO were suppressed by VNS. Moreover, VNS promoted the pro-to-anti-inflammatory response of microglia and inhibited activation of astrocytes, while silencing of USP10 prevented the neuroprotective and anti-neuroinflammatory effects of VNS.ConclusionUSP10 is a potential mediator for VNS to alleviate neurological deficits, neuroinflammation, and glial cell activation in ischaemic stroke by inhibiting NF-κB signalling pathway.
Knowledge mapping and research trends of IL-33 from 2004 to 2022: a bibliometric analysis
Authors: Jin Jingyi , Wan Yantong , Shu Qiang , Liu Jinghua , Lai Dengming 
Published: April 2023

BackgroundIL-33 has been studied widely but its comprehensive and systematic bibliometric analysis is yet available. The present study is to summarize the research progress of IL-33 through bibliometric analysis.MethodsThe publications related to IL-33 were identified and selected from the Web of Science Core Collection (WoSCC) database on 7 December 2022. The downloaded data was analyzed with bibliometric package in R software. CiteSpace and VOSviewer were used to conduct IL-33 bibliometric and knowledge mapping analysis.ResultsFrom 1 January 2004 to 7 December 2022, 4711 articles on IL-33 research published in 1009 academic journals by 24652 authors in 483 institutions from 89 countries were identified. The number of articles had grown steadily over this period. The United States of America(USA) and China are the major contributors in the field of research while University of Tokyo and University of Glasgow are the most active institutions. The most prolific journal is Frontiers in Immunology, while the Journal of Immunity is the top 1 co-cited journal. Andrew N. J. Mckenzie published the most significant number of articles and Jochen Schmitz was co-cited most. The major fields of these publications are immunology, cell biology, and biochemistry &amp; molecular biology. After analysis, the high-frequency keywords of IL-33 research related to molecular biology (sST2, IL-1), immunological effects (type 2 immunity, Th2 cells), and diseases (asthma, cancer, cardiovascular diseases). Among these, the involvement of IL-33 in the regulation of type 2 inflammation has strong research potential and is a current research hotspot.ConclusionThe present study quantifies and identifies the current research status and trends of IL-33 using bibliometric and knowledge mapping analysis. This study may offer the direction of IL-33-related research for scholars.
Decreased feeding rates of the copepod Acartia tonsa when exposed to playback harbor traffic noise
Authors: Kühn Saskia , King Franziska , Heubel Katja 
Published: April 2023

IntroductionCopepods present the largest and most diverse group of zooplankton and their feeding behavior can affect top-down and bottom-up processes. Thus, how efficient feeding is executed determines the abundance of copepods’ prey and their predators and, with that, carbon transfer and storage in ecosystems. The rise of anthropogenic underwater noise from shipping, oil exploration and exploitation, wind farm construction and operation, and more, is increasingly changing the marine acoustic environment. This acoustic pollution can have detrimental effects on biological life. Studies on this topic increasingly indicate that anthropogenic underwater noise adversely affects primary producers, marine mammals, fish, and invertebrates. However, little data exist on the effects of anthropogenic underwater noise on the feeding behavior of zooplankton.MethodsHere, we investigated the ingestion and clearance rates of the copepodAcartia tonsaon a motile phytoplankton as a function of prey density under ambient aquarium sound conditions and, when exposed to playback, harbor traffic noise.ResultsWe measured significantly decreased ingestion rates and clearance rates ofA. tonsawhen exposed to harbor noise compared to ambient conditions. The negative impact of noise on the ingestion rates was found at all given phytoplankton cell densities between 1k to 10k cells ml−1. Clearance rates were fitted to the Rogers random predator equation which revealed significantly decreased capture rates on phytoplankton under the exposure of harbor noise while handling times remained the same in both sound treatments.DiscussionOur results call for follow-up studies to focus on noise driven community-effects in field experiments to confirm laboratory results and to predict the outcome of a changing world with multiple stressors. Further, the underlying mechanism on how noise affects the feeding behavior of copepods is still unknown. Noise may distract copepods or mask hydromechanical cues of the prey. Noise may also adversely affect copepod physiology or morphology that would lead to changes in the feeding behavior. All potential mechanisms need to be investigated rigorously in future experiments.
Assessment of risk scores to predict mortality of COVID-19 patients admitted to the intensive care unit
Authors: Nogueira Matheus Carvalho Alves , Nobre Vandack , Pires Magda Carvalho , Ramos Lucas Emanuel Ferreira , Ribeiro Yara Cristina Neves Marques Barbosa , Aguiar Rubia Laura Oliveira , Vigil Flavia Maria Borges , Gomes Virginia Mara Reis , Santos Camila de Oliveira , Miranda Davi Mesquita , Durães Pamela Andrea Alves , Costa Josiane Moreira da , Schwarzbold Alexandre Vargas , Gomes Angélica Gomides dos Reis , Pessoa Bruno Porto , Matos Carolina Cunha , Cimini Christiane Corrêa Rodrigues , Carvalho Cíntia Alcântara de , Ponce Daniela , Manenti Euler Roberto Fernandes , Cenci Evelin Paola de Almeida , Anschau Fernando , Costa Flávia Carvalho Cardoso , Nascimento Francine Janaina Magalhães , Bartolazzi Frederico , Grizende Genna Maira Santos , Vianna Heloisa Reniers , Nepomuceno Jomar Cristeli , Ruschel Karen Brasil , Zandoná Liege Barella , Castro Luís César de , Souza Maíra Dias , Carneiro Marcelo , Bicalho Maria Aparecida Camargos , Vilaça Mariana do Nascimento , Bonardi Naiara Patrícia Fagundes , Oliveira Neimy Ramos de , Lutkmeier Raquel , Francisco Saionara Cristina , Araújo Silvia Ferreira , Delfino-Pereira Polianna , Marcolino Milena Soriano 
Published: April 2023

ObjectivesTo assess the ABC2-SPH score in predicting COVID-19 in-hospital mortality, during intensive care unit (ICU) admission, and to compare its performance with other scores (SOFA, SAPS-3, NEWS2, 4C Mortality Score, SOARS, CURB-65, modified CHA2DS2-VASc, and a novel severity score).Materials and methodsConsecutive patients (≥ 18 years) with laboratory-confirmed COVID-19 admitted to ICUs of 25 hospitals, located in 17 Brazilian cities, from October 2020 to March 2022, were included. Overall performance of the scores was evaluated using the Brier score. ABC2-SPH was used as the reference score, and comparisons between ABC2-SPH and the other scores were performed by using the Bonferroni method of correction. The primary outcome was in-hospital mortality.ResultsABC2-SPH had an area under the curve of 0.716 (95% CI 0.693–0.738), significantly higher than CURB-65, SOFA, NEWS2, SOARS, and modified CHA2DS2-VASc scores. There was no statistically significant difference between ABC2-SPH and SAPS-3, 4C Mortality Score, and the novel severity score.ConclusionABC2-SPH was superior to other risk scores, but it still did not demonstrate an excellent predictive ability for mortality in critically ill COVID-19 patients. Our results indicate the need to develop a new score, for this subset of patients.
A new multidimensional group intervention for cognitive and psychosocial functioning for older adults: Background, content, and process evaluation
Authors: Chadjikyprianou Andreas , Constantinidou Fofi 
Published: April 2023

IntroductionAn essential element of quality aging is the maintenance of cognitive and psychosocial functioning. The principal objective of the present paper was to present the theoretical framework, content and process evaluation of a newly developed multi-dimensional group intervention designed to strengthen/improve areas of cognitive and psychosocial functioning in adults over 65.MethodsThe intervention implements multiple methodologies aiming to facilitate contextual integration of learned concepts and strategies derived from clinical psychology and rehabilitation. It moves seamlessly on the cognition–emotion axes and consists of five active ingredients selected to address challenges associated with aging: Memory Compensatory Strategies, Problem-Solving, Emotion Regulation, Mindfulness, and Locus of Control. Thirty participants joined the intervention group aged 65–75  years (M = 69.03; SD = 3.04). All 30 participants who were included in the intervention group completed the program.ResultsResults from the Participant Satisfaction Scale indicate that the program was perceived very positively by participants, who also reported implementing their newly learned strategies in activities of daily life. Furthermore, there was high correlation between internal locus of control and the learned strategies.DiscussionThe outcomes of this analysis indicate that the intervention is feasible and well tolerated by our target group. This multidimensional intervention may offer a valuable contribution to public health care and dementia prevention for older adults.Clinical Trial Registration[https://clinicaltrials.gov/ct2/results?cond=NCT01481246], identifier [NCT01481246].
Alleviation of DSS-induced colitis in mice by a new-isolated Lactobacillus acidophilus C4
Authors: Liu Qianqian , Jian Wenwen , Wang Lu , Yang Shenglin , Niu Yutian , Xie ShuaiJing , Hayer Kim , Chen Kun , Zhang Yi , Guo Yanan , Tu Zeng 
Published: April 2023

IntroductionProbiotic is adjuvant therapy for traditional drug treatment of ulcerative colitis (UC). In the present study, Lactobacillus acidophilus C4 with high acid and bile salt resistance has been isolated and screened, and the beneficial effect of L. acidophilus C4 on Dextran Sulfate Sodium (DSS)-induced colitis in mice has been evaluated. Our data showed that oral administration of L. acidophilus C4 remarkably alleviated colitis symptoms in mice and minimized colon tissue damage.MethodsTo elucidate the underlying mechanism, we have investigated the levels of inflammatory cytokines and intestinal tight junction (TJ) related proteins (occludin and ZO-1) in colon tissue, as well as the intestinal microbiota and short-chain fatty acids (SCFAs) in feces.ResultsCompared to the DSS group, the inflammatory cytokines IL-1β, IL-6, and TNF-α in L. acidophilus C4 group were reduced, while the antioxidant enzymes superoxide dismutase (SOD), glutathione (GSH), and catalase (CAT) were found to be elevated. In addition, proteins linked to TJ were elevated after L. acidophilus C4 intervention. Further study revealed that L. acidophilus C4 reversed the decrease in intestinal microbiota diversity caused by colitis and promoted the levels of SCFAs.DiscussionThis study demonstrate that L. acidophilus C4 effectively alleviated DSS-induced colitis in mice by repairing the mucosal barrier and maintaining the intestinal microecological balance. L. acidophilus C4 could be of great potential for colitis therapy.
Hybridization alters the gut microbial and metabolic profile concurrent with modifying intestinal functions in Tunchang pigs
Authors: He Jiayi , Zhang Yunchao , Li Hui , Xie Yanshe , Huang Guiqing , Peng Chen , Zhao Pengju , Wang Zhengguang 
Published: April 2023

IntroductionHybridization has been widely used among Chinese wild boars to improve their growth performance and maintain meat quality. Most studies have focused on the genetic basis for such variation. However, the differences in the gut environment between hybrid and purebred boars, which can have significant impacts on their health and productivity, have been poorly understood.MethodsIn the current study, metagenomics was used to detect the gut microbial diversity and composition in hybrid Batun (BT, Berkshire × Tunchang) pigs and purebred Tunchang (TC) pigs. Additionally, untargeted metabolomic analysis was used to detect differences in gut metabolic pathways. Furthermore, multiple molecular experiments were conducted to demonstrate differences in intestinal functions.ResultsAs a result of hybridization in TC pigs, a microbial change was observed, especially in Prevotella and Lactobacillus. Significant differences were found in gut metabolites, including fatty acyls, steroids, and steroid derivatives. Furthermore, the function of the intestinal barrier was decreased by hybridization, while the function of nutrient metabolism was increased.DiscussionEvidences were shown that hybridization changed the gut microbiome, gut metabolome, and intestinal functions of TC pigs. These findings supported our hypothesis that hybridization altered the gut microbial composition, thereby modifying the intestinal functions, even the host phenotypes. Overall, our study highlights the importance of considering the gut microbiome as a key factor in the evaluation of animal health and productivity, particularly in the context of genetic selection and breeding programs.