bluuebunny/crossref_metadata_2025_split huggingface dataset:
A nationwide cross-sectional survey of prevalence and association of physical activity with suicidal, psychosocial, and risky-health indicators among adolescents in Bangladesh
Authors: KHAN MD. JOBAIR , n/a Sayma , Ganesan Balasankar , Ali Mohammed Usman , Abdullahi Auwal , Hasan S. M. Mahmudul , Khan Atiqur Rahman , Hawlader Mohammad Delwer Hossain , Winser Stanley John 
Published: August 2023

Abstract
Background
Physical activities among adolescents are limited, and suicidal, psychosocial, and risky health indicators are alarmingly rising in low-resource countries, including Bangladesh. Thus, this study investigates the prevalence, associations and relative contributors of physical activity with suicidal, psychosocial, risky-health indicators.
Methods
This is a secondary analysis of GSHS database. A total of 2058 adolescents in grades 7 to 10, aged 11 to 17 years completed a self-administered questionnaire. Sex-stratified logistic regression was utilized to evaluate associations between physical activity and suicidal, psychological, and risky-health indicators. A two-step cluster analysis was performed to identify the risky indicators. Hierarchical regression examined the aforementioned factors' contributions to physical activity.
Results
Half of the adolescents (boys 18.9% vs. girls 30.3%) indicated satisfactory physical activity following WHO guidelines. Most active group of frequency of activity and walking or bicycle to school were positively associated with suicidal behavior. Isolated adolescents suffered anxiety despite frequent engagement in physical activity. Reduced frequency of physical activity was significantly associated with sexual activity, smoking, tobacco product usage (girls), alcohol abuse (boys), and ever-abusing drug use (boys and girls). Cluster analysis revealed girls and boys were predisposed to physical abuse and bully, respectively. Hierarchical regression models showed a significant contribution of selected predictors to physical activity.
Conclusion
Increased physical activity through regular walking and bicycling may lower suicidal behavior among adolescents. The most active groups of physical activity were associated with predictors and varied by sex; boys were bullied, while girls were subjected to physical abuse.
Cost-utility analysis of palivizumab for preventing respiratory syncytial virus in preterm neonates and infants in Colombia
Authors: Ordóñez Jaime E. , Huertas Victor M. 
Published: August 2023

Abstract
Aim.
Palivizumab has proven effective in reducing hospitalizations, preventing severe illness, improving health outcomes, and reducing healthcare costs for infants at risk of respiratory syncytial virus (RSV) infection. We aim to assess the value of palivizumab in preventing RSV infection in high-risk infants in Colombia, where RSV poses a significant threat, causing severe respiratory illness and hospitalizations.
Methods.
We conducted a decision tree analysis to compare five doses of palivizumab with no palivizumab. The study considered three population groups: preterm neonates (≤ 35 weeks gestational age), infants with bronchopulmonary dysplasia (BPD), and infants with hemodynamically significant congenital heart disease (CHD). We obtained clinical efficacy data from IMpact-RSV and Cardiac Synagis trials, while we derived neonatal hospitalization risks from the SENTINEL-1 study. We based hospitalization and recurrent wheezing management costs on Colombian analyses and validated them by experts. We estimated incremental cost-effectiveness ratios and performed 1,000 Monte Carlo simulations for probabilistic sensitivity analyses.
Results.
Palivizumab is a dominant strategy for preventing RSV infection in preterm neonates and infants with BPD and CHD. Its high efficacy (78% in preventing RSV in preterm infants), the substantial risk of illness and hospitalization, and the high costs associated with hospitalization, particularly in neonatal intensive care settings, support this finding. The scatter plots and willingness-to-pay curves align with these results.
Conclusion.
Palivizumab is a cost-saving strategy in Colombia, effectively preventing RSV infection in preterm neonates and infants with BPD and CHD by reducing hospitalizations and lowering healthcare costs.
Validation of Chinese version of the 5-Item WHO Well-Being Index in type 2 diabetes mellitus patients
Authors: Du Jianhua , Jiang Yinan , Lloyd Cathy , Sartorius Norman , Ren Jie , Zhao Weigang , Wei Jing , Hong Xia 
Published: August 2023

Abstract
Background
For better disease management and improved prognosis, early identification of co-morbid depression in diabetic patients is warranted. the WHO-5 well-being index (WHO-5) has been used to screen for depression in diabetic patients, and its Chinese version (WHO-5-C) has been validated. However, its psychometric properties remain to be further validated in the type 2 diabetes patient population. The aim of our study was to examine the reliability and validity of the WHO-5-C in patients with type 2 diabetes mellitus.
Methods
The cross-sectional study was conducted on 200 patients from July 2014 to March 2015. All patients should complete the WHO-5-C, the Patient Health Questionnaire-9 (PHQ-9), the 20-item problem areas in diabetes (PAID-20), the Mini International Neuropsychiatric Interview (M.I.N.I), and Hamilton Rating Scale for Depression (HAM-D). Internal consistency of WHO-5 was revealed by Cronbach’s alpha, and constructive validity by confirmatory factor analysis (CFA). Relationship with PHQ-9, HAM-D and PAID-20 was examined for concurrent validity, and ROC analysis was performed for criterion validity.
Results
The WHO-5-C presented satisfactory reliability (Cronbach’s alpha = 0.88). CFA confirmed the unidimensional factor structure of WHO-5-C. The WHO-5-C had significant negative correlation with HAM-D (r=-0.610), PHQ-9 (r=-0.694) and PAID-20 (r=-0.466), confirming good concurrent validity. Using M.I.N.I as the gold standard, the cut-off value of WHO-5-C was 42, with a sensitivity of 0.83 and specificity of 0.75.
Conclusion
The WHO-5-C holds satisfactory reliability and validity that is suitable for depression screening in type 2 diabetes patients as a short and convenient instrument.
Prevalence of potentially inappropriate medications in Iranian elderly hospitalized patients
Authors: Kermani Ali Sharifzadeh , Borhaninejad Vahidreza , Sepehri Gholamreza , Momeni Mohsen , Danaei Mina 
Published: August 2023

Abstract
Background: Population aging is a health challenge in the world and Iran. Potentially inappropriate medications (PIMs) should not be prescribed to the elderly due to low effectiveness and adverse drug reactions. There are various tools for screening PIMs including Zhan's criteria. This study was conducted to determine the frequency of PIMs in the elderly admitted to teaching hospitals in Kerman, in 2023.
Methods: This cross-sectional descriptive study was conducted on 1500 elderly patients hospitalized in educational hospitals in Kerman, Iran, 2023. The inclusion criteria were determined as age> 60 years. Patients with incomplete electronic information were excluded from the study. Patients were selected using stratified multistage sampling method. The data collection forms were completed according to the Health Information System (HIS) of the hospital, which includes the patient's age and gender, drug information, and the name of the hospital and ward. PIMs prescriptions were assessed according to Zhan's criteria. Statistical analyses were performed using SPSS version 20.0. The results were presented as mean ± standard deviation (SD) for quantitative data and frequencies (percents) for qualitative data.
Results: Nearly 17.79% of patients were treated with at least one PIMs included in the Zhan criteria. Among them, one PIMs was found in 76.02%, two PIMs were detected in 19.86%, and three or more PIMs were found in 4.12% of patient’s records. Patients who were admitted to the surgical ward (47.5%), Critical Care Unit (34.2%), and infectious diseases ward (25.3) had the most percent of prescribing PIMs. Totally, 331 PIMs were used for patients. Considering the classification of Zhan, 43.51%, 23.26%, and 33.23% of drugs were classified as always avoid, rarely appropriate, and some indication, respectively. Considering pharmaceutical categories, sedative-hypnotics, opioids, and antihistamines were the most prevalent PIMs. Among inappropriate drugs, pethidine, chlordiazepoxide, Hyoscine, Chlorpheniramine, and Indomethacin were the most prevalent PIMs, respectively.
Conclusions: PIMs are still frequently experienced by elderly patients in Iranian hospitals. Specifically, physicians in the surgical ward, Critical Care Unit, and infectious diseases ward need to be more aware of PIMs. More attention should be paid to sedative-hypnotics, opioids, and antihistamines as the most frequently prescribed PIMs.
Loss of DRD5P2 in hypoxia attenuates ROCK2 degradation to promote EMT and gastric cancer metastasis
Authors: Yu Zhenjia , Pan Tao , Wang Xiaofeng , Jin Zhijian , Lu Yifan , Wu Xiongyan , Hou Junyi , Wu Airong , Li Zhen , Chang Xinyu , Zhou Quan , Li Jianfang , Liu Wentao , Ni Zhentian , Yang Zhongyin , Li Chen , Yan Min , Liu Bingya , Yan Chao , Zhu Zhenggang , Su Liping 
Published: August 2023

Abstract
Background: Metastasis is the leading cause of gastric cancer (GC)-related death. However, the molecular mechanisms underlying GC metastasis are not well understood. In this study, we focus on DRD5P2, a novel long non-coding RNA, in GC metastasis.
Methods: Expression of DRD5P2 in GC is detected by RT-PCR and FISH. The effect of DRD5P2 in GC cells were examined by transwell invasion and migration assays. The pathways underlying DRD5P2/ROCK2 signaling were studied by Western blot, Co-IP, Ch-IP and RIP analysis. Regulatory mechanism between hypoxia and DRD5P2 expression were explored in vitro by Ch-IP and Dual-luciferase reporter assays.
Results: DRD5P2 expression is down-regulated in advanced human GC and is associated with poor clinical outcomes. Gain- and loss- of function studies show that DRD5P2 inhibits GC cell migration, invasion, and EMT in vitro, as well as peritoneal dissemination in vivo. Mechanistic analysis reveals that DRD5P2 binds with ROCK2 and recruits the E3-ubiquitin ligase KAP1 to mediate ROCK2 degradation, thus suppressing the Ezrin/HRAS/ERK/CREB pathway and ultimately attenuating Snail-mediated EMT and GC metastasis. Furthermore, DRD5P2 transcription is inhibited by hypoxia in a HIF-1α/ZNF263-dependent manner in GC cells.
Conclusions: DRD5P2 acts as a tumor suppressor in GC metastasis by suppressing ROCK2/ERK/Snail signaling, and DRD5P2 is transcriptionally suppressed under hypoxia via HIF-1α/ZNF263 axis.
Population genomics and epigenomics provide insights into the evolution of facultative asexuality in plants
Authors: Xu Shuqing , Wang Yangzi , Pablo Duchen , Chávez Alexandra , S K. Sowjanya , Appenroth Klaus , Zhao Hai , Widmer Alex , Huber Meret 
Published: August 2023

Abstract

Many organisms, particularly plants, can reproduce both sexually and asexually but with varying frequencies. Because variations in the reproductive system profoundly affect fitness and genomic diversity, natural selection should act on changes between sexual and asexual types of propagation. However, the genomic evidence of natural selection driving switches between reproductive systems remains scarce, and the evolutionary consequences of such switches on the genomic and epigenomic diversity remain controversial. Here, by analyzing population genomes and epigenomes of Spirodela polyrhiza (L.) Schleid., a facultatively asexual angiosperm, we show that while the genomic diversity and DNA methylation levels are very low, they vary among populations. These variations are associated with both the demographic history and the frequency of sexual reproduction in each population. A genome-wide scan revealed that multiple genes involved in flowering and embryogenesis were under positive selection, consistent with the hypothesis that natural selection drove the evolution of asexuality in this plant. Together, these results provide new insights into the evolution of plant reproductive systems and suggest that natural selection can drive the evolutionary changes of asexuality, which in turn alters the levels of genomic and epigenomic diversity.
The disparity in hesitancy toward COVID-19 vaccination between older adults in care homes and those in the community in Taizhou, China
Authors: Wu Xudong , Lv Li , Yan Huanjun , Zhao Shuangying , Zhang Xiaodong , Zhu Kelei 
Published: August 2023

Abstract
Older adults are priority coronavirus disease 2019 (COVID-19) vaccine recipients. Our aim was to investigate the prevalence of and factors influencing vaccine hesitancy in older adults living in care homes and communities. A self-administered COVID-19 vaccine hesitancy survey was conducted from September 2021 to December 2021 among people aged ≥60 years in eight care homes (382 participants) and the community (112 participants) in Taizhou, China. We found that 58.1% of the older adults in care homes and 36.6% of those in the community were hesitant to receive the COVID-19 vaccine and that there was a statistically significant difference (P<0.001). Multiple logistic regression results indicated that the main factors influencing hesitation among the older adults in care homes were being male (OR=1.67, 95% CI: 1.01-2.76); their cognitive level, including having a high perceived risk of COVID-19 infection (OR=3.06, 95% CI: 1.73-5.43) or the perception of low vaccine safety (OR=3.08, 95% CI: 1.545- 6.145); anxiety (OR=3.43, 95% CI: 1.96-5.99); and no previous influenza vaccination (OR=1.82, 95% CI: 1.13-2.93); whereas those for older adults in the community were comorbid chronic diseases (OR=3.13, 95% CI: 1.11- 8.78) and community workers not recommending the vaccine (OR=8.223, 95% CI: 1.77-38.27). The proportion of older adults in care homes who were hesitant to receive the COVID-19 vaccine was significantly higher than for older adults in the community. Targeted measures should be implemented to reduce vaccine hesitancy and improve vaccination rates in response to the special environment of care homes and the characteristics of this population.
Molecular patterns of resistance to immune checkpoint blockade in melanoma
Authors: Jonsson Goran , Lauss Martin , Phung Bengt , Borch Troels , Harbst Katja , Kaminska Kamila , Ebbesson Anna , Hedenfalk Ingrid , Yuan Joan , Nielsen Kari , Ingvar Christian , Carneiro Ana , Isaksson Karolin , Pietras Kristian , Svane Inge Marie , Donia Marco 
Published: August 2023

Abstract
Immune checkpoint blockade (ICB) has improved outcome for metastatic melanoma patients but not all benefit from treatment. Several immune- and tumor intrinsic features are associated with clinical response at baseline. However, we need to further understand the molecular changes occurring during development of ICB resistance. Here, we collected biopsies from a unique cohort of 44 melanoma patients after progression to anti-CTLA4 or anti-PD1 monotherapy. Genetic alterations of antigen presentation and interferon gamma signaling pathways were observed in approximately 25% of ICB resistant cases. Anti-CTLA4 resistant lesions had a sustained immune response, including immune-regulatory features, as suggested by multiplex spatial and TCR clonality analyses. One anti-PD1 resistant lesion harbored a distinct immune cell niche, however, anti-PD1 resistant tumors were generally immune poor with non-expanded TCR clones. Such immune poor microenvironments were associated with melanoma cells having a de-differentiated phenotype lacking expression of MHC-I molecules. In addition, anti-PD1 resistant tumors had reduced fractions of PD1+ CD8+ T cells as compared to ICB naïve metastases. Collectively, these data show the complexity of ICB resistance and highlight differences between anti-CTLA4 and anti-PD1 resistance that may underlie differential clinical outcomes of therapy sequence and combination.
Utilizing a composite citation index for evaluating clinical ophthalmology research: insights into gender, nationality, and self-citation among top ophthalmology researchers
Authors: Pan Annabelle , Pham Alex , Appelo Ben , Legault Gary , Woreta Fasika , Justin Grant 
Published: August 2023

Abstract
Objectives
To compare the performance of a composite citation score (c-score) and its six constituent citation indices, including H-index, in assessing research impact in the field of ophthalmology. Secondarily, to explore career and demographic characteristics of the most highly cited researchers in ophthalmology.
Methods
A publicly available database was accessed to compile a set of top researchers in the field of clinical ophthalmology and optometry based on Scopus data from 1996-2021. Each citation index was evaluated against the Weisenfeld Award in Ophthalmology, presented by the Association for Research in Vision and Ophthalmology (ARVO) and used in this study as a “ground truth” reference for identifying authors with high research impact. Secondary analyses investigated authors’ self-citation rates, career length, gender, and country affiliation.
Results
Approximately one thousand unique authors publishing primarily in clinical ophthalmology/optometry were listed in the Scopus-derived database. The c-score consistently included the highest number of Weisenfeld awardees in its top ranks, outperforming other citation indices. Superior ranking by c-score was weakly associated with higher self-citation rate. The top 50 researchers by c-score had significantly longer career lengths compared to other ophthalmology researchers. Overall, 16 percent of authors in the database were identified as female, and 64 percent were affiliated with the United States of America. The average c-score did not differ between female and male authors.
Conclusion
The c-score is an effective metric for assessing research impact in ophthalmology by capturing more Weisenfeld awardees compared to commonly used citation indices.
The fertilizing intention of married childless women and its influencing factors -----a cross-sectional study in China after Two-child policy
Authors: Zhao Xuanli , Wang Ruixian , Ye Ding , Jing Fangyuan 
Published: August 2023

Abstract
Background: To investigate the fertilizing intention of these married childless women and the its influencing factors.
Methods: Married women of childbearing age (20-49 years old) who had been married and without biological child for more than 1 years (Married Childless Women, MCW) in Zhejiang, China were recruited and face-to-face interviewed for their fertilizing intention and possible influencing factors. Unconditional Multivariable logistic regression was used to assess the associations between potential influencing factors and fertilizing intention.
Results: Overall, 22.14% of study population were not willing to have child. Furthermore, approximately 40% of these “married childless women with fertilizing intention” wished to have only one child in their lives. Fertilizing intention rate of MCWs went up first before the age of 35, then it decreased dramatically with increasing age. Influencing factors of MCWs’ fertilizing intention included age, duration of marriage (OR: 1.1; 95% CI: 1.08-1.13), more marriages (OR: 1.4; 95% CI:1.06-1.84), residential location (OR: 0.76; 95% CI: 0.65-0.90), education level (OR: 1.28; 95% CI: 1.04-1.22), pregnant history (OR: 0.43; 95% CI: 0.28-0.65) abnormal menstrual blood volume (either heavy(OR: 0.81; 95% CI: 0.66-0.98), light(OR: 0.66; 95% CI: 0.51-0.84), appropriate age of birth considered by MCW (OR: 1.13; 95% CI 0.94-1.35) and appropriate age of marriage considered by MCW (OR: 1.32; 95% CI: 0.92-1.93). Women with higher education level (OR: 1.27; 95% CI: 1.05-1.53), Coming from rural area (OR: 1.23; 95% CI: 1.07-1.43), and having an irregular menstruation (OR: 1.34; 95% CI: 1.15-1.56) tend to want more children when compared with the reference group.
Conclusion: After the implementation of “Two Child Policy”, the fertilizing intention rate of married childless women in Zhejiang, China was 77.86%. Of the cohort of married childless women who expressed a desire to conceive, almost 40% indicated a preference for having a single child. The influencing factors that affect the fertilizing intention should be carefully considered by policy makers when developing effective reproductive health and family planning policies. This approach will ensure that policies are tailored to meet the needs and preferences of married childless women.
A comprehensive study on viral nanoparticles: Synthesis, solution state structural properties using dynamic light scattering and its interaction with host bacterium
Authors: Manoswini Manoswini , Mohanty Madhuchhanda , Majumdar Amrito Ghosh , Sahu Bikash , Panda Subrat Kumar , Mohapatra Rajanikant , MOHANTY Priti Sundar 
Published: August 2023

Abstract
Bacterium-virus interactions are very popular in infectious diseases and the viral particles (also called bacteriophages or phages) have a wide range of applications in therapeutics, food industry and agriculture. In-spite of their huge popularity both in fundamental and industrial sectors, there are still challenges with respect to purification, and scaling up processes due to their aggregating nature in solutions. Viral aggregates not only increase their survival efficiency against disinfectants, but also strongly affects their interactions with host bacterium. As the dimension of virus particle falls in the nanometer range, and they undergo Brownian motion in solution, non-invasive popular method like dynamic light scattering can be implemented to evaluate the structural properties of viral particles in the solution state at each step of purification process. In this context, we have carried out systematic studies on a popular model virus particles, P22. These include synthesis of phages and their biological characterizations such as spot test, growth kinetics, specificity test. The solution state structures are characterized before and after purification using extensive using extensive dynamic light scattering. Our studies result in a higher yield of virus particles and above a threshold concentration ratio of virus-to-bacteria, bacterial lysis occurs. Purified viral solution reveals coexistence of two translation diffusion coefficients with hydrodynamics radius of 40 nm and 300 nm respectively. These sizes correspond to single viral particles (20%) coexisting with 80% of higher order structural (multiple viral particles). The affinity study demonstrates a 60 to 70% efficiency of virus binding with their host bacterium. In-silico molecular docking explain the role of different types of molecular interactions between the tail spike protein (TSP) of virus and the o-antigen of the host bacterium. The Van der Waal-hydrophobic-desolvation (Vhd) interaction is the dominant one due to which phage strongly binds to host bacteria. Our methodology can be helpful formulation of different types of viral systems.