bluuebunny/crossref_metadata_2025_split huggingface dataset:
Supplementary Figures from Targeting NAD<sup>+</sup>/PARP DNA Repair Pathway as a Novel Therapeutic Approach to <i>SDHB</i>-Mutated Cluster I Pheochromocytoma and Paraganglioma
Authors: Pang Ying , Lu Yanxin , Caisova Veronika , Liu Yang , Bullova Petra , Huynh Thanh-Truc , Zhou Yiqiang , Yu Di , Frysak Zdenek , Hartmann Igor , Taïeb David , Pacak Karel , Yang Chunzhang 
Published: April 2023

<p>Supplementary Figures S1 A.	Quantitative PCR showed CA9, PNMT, and VEGFA expression in MTT cells. Higher expressions of hypoxia related genes were detected in SDHBKD cells. B.	NAD+ quantification confirmed depletion of NAD+ by Nampt inhibitors FK866 and CHS828. C.	Blue native gel analysis on mitochondrial complex formation in MTT cells (left panel). Mitochondrial complex I formation is increased in SDHBKD cells. In gel enzyme activity assay (IGA) showed elevated mitochondrial complex I activity in SDHBKD cells (right panel). D.	Re-expression of SDHB in MTT cells (upper panel). The re-expression of SDHB reduced pADPR formation in SDHBKD cells, with a corresponding increased of Î³H2A.X expression (lower panel). E.	Quantification of NAD+/NADH ratio in MTT cells. Re-expression of SDHB partially reversed the elevated NAD+/NADH ratio in SDHBKD cells. F.	Quantification of flow cytometry data. A significant G2/M arrest is seen in combination treated group. G.	Western blot for MGMT detection in MTT cells. No MGMT expression was seen in either SDHBWT or SDHBKD cells.ï€ ï�¢-actin was used as loading control. H.	Cell viability test showing enhanced cytotoxic effect of combination treatment. **p<0.01. I.	In vivo bioluminescence showing significantly reduced metastatic lesion with TMZ and combination treatments. *p<0.05, **p<0.01.</p>
Figure S5 from <i>SMAD4</i> Gene Mutation Renders Pancreatic Cancer Resistance to Radiotherapy through Promotion of Autophagy
Authors: Wang Feng , Xia Xiaojun , Yang Chunying , Shen Jianliang , Mai Junhua , Kim Han-Cheon , Kirui Dickson , Kang Ya'an , Fleming Jason B. , Koay Eugene J. , Mitra Sankar , Ferrari Mauro , Shen Haifa 
Published: April 2023

<p>Figure S5. SMAD4-induced autophagy is partially rescued by ROS scavenger. (A)	Panc-1 cells with Smad4 knockdown were treated with NAC, irradiation (IR), or NAC plus IR. Cell lysates were harvested and blotted with different antibodies indicated. (B) Panc-1 cells with Smad4 knockdown were treated with medium (Control), NAC, or CQ. Cell lysates were harvested and blotted with different antibodies as indicated.</p>
Figure S4 from <i>SMAD4</i> Gene Mutation Renders Pancreatic Cancer Resistance to Radiotherapy through Promotion of Autophagy
Authors: Wang Feng , Xia Xiaojun , Yang Chunying , Shen Jianliang , Mai Junhua , Kim Han-Cheon , Kirui Dickson , Kang Ya'an , Fleming Jason B. , Koay Eugene J. , Mitra Sankar , Ferrari Mauro , Shen Haifa 
Published: April 2023

<p>Figure S4. SMAD4-induced IR-resistance is partially rescued by pretreatment of autophagy inhibitor or ROS scavenger. (A)	Immunoblot of SMAD4 was measured using antibody against SMAD4 in Panc-1-CTL, Panc-1-sh-SMAD4-#2 Cells. (B-C) Panc-1-CTL, Panc-1-sh-SMAD4-#2 Cells were pretreated with 10μM chloroquine (CQ) (B) or 3mM N-acetyl-L-cysteine (NAC) (C) for 1 h, and then treated with indicated doses of IR and colony formation assays were conducted with more than 50 cells counted. Shown are the averages of triplicate samples. Standard errors are shown by error bars. * , P < .05, **, P < .001</p>
Supp. Figure 3 from Expression of PD-1 by T Cells in Malignant Glioma Patients Reflects Exhaustion and Activation
Authors: Davidson Tom B. , Lee Alexander , Hsu Melody , Sedighim Shaina , Orpilla Joey , Treger Janet , Mastall Max , Roesch Saskia , Rapp Carmen , Galvez Mildred , Mochizuki Aaron , Antonios Joseph , Garcia Alejandro , Kotecha Nikesh , Bayless Nicholas , Nathanson David , Wang Anthony , Everson Richard , Yong William H. , Cloughesy Timothy F. , Liau Linda M. , Herold-Mende Christel , Prins Robert M. 
Published: April 2023

<p>Supplementary Figure 3: Gene expression analysis shows increased transcription of activation and exhaustion genes in the PD-1+ population in glioma TILs and of antigen experienced genes in the PD-1+ population in glioma PBMCs (each dot represents a distinct patient). A)	Fold change of various exhaustion markers between PD-1+ and PD-1- cells in glioma TILs and PBMCs. B)	Fold change of various activation markers between the PD-1+ and PD-1- cells in glioma TILs and PBMCs. C)	Fold change of various memory markers between the PD-1+ and PD-1- cells in glioma TILs and PBMCs.</p>
Supp. Figure 2 from Expression of PD-1 by T Cells in Malignant Glioma Patients Reflects Exhaustion and Activation
Authors: Davidson Tom B. , Lee Alexander , Hsu Melody , Sedighim Shaina , Orpilla Joey , Treger Janet , Mastall Max , Roesch Saskia , Rapp Carmen , Galvez Mildred , Mochizuki Aaron , Antonios Joseph , Garcia Alejandro , Kotecha Nikesh , Bayless Nicholas , Nathanson David , Wang Anthony , Everson Richard , Yong William H. , Cloughesy Timothy F. , Liau Linda M. , Herold-Mende Christel , Prins Robert M. 
Published: April 2023

<p>Supplementary Figure 2: Additional CyTOF data A)	The percentage of the CD3+ PD-1+ cells from the TIL (green dots) and PBMCs (red dots) in each cluster (*P<0.05, ***P{less than or equal to}0.0005). Each dot represents a single patient. B)	The median expression level of CD45RO in a subset of 10 patients (*P<0.05). C)	The median expression level of CD39 in a subset of 4 patients (***P{less than or equal to}0.0005). D)	The median expression level of TIGIT in a subset of 4 patients.</p>
Supplementary Data from Significance of <i>BRCA2</i> and <i>RB1</i> Co-loss in Aggressive Prostate Cancer Progression
Authors: Chakraborty Goutam , Armenia Joshua , Mazzu Ying Z. , Nandakumar Subhiksha , Stopsack Konrad H. , Atiq Mohammad O. , Komura Kazumasa , Jehane Lina , Hirani Rahim , Chadalavada Kalyani , Yoshikawa Yuki , Khan Nabeela A. , Chen Yu , Abida Wassim , Mucci Lorelei A. , Lee Gwo-Shu Mary , Nanjangud Gouri J. , Kantoff Philip W. 
Published: April 2023

<p>â€¢	Figure legends S1 to S6. â€¢	Tables S1 and S4, part of Table S8, and Tables S9, S10, and S11.</p>
Supplementary Information from A Chimeric Signal Peptide–Galectin-3 Conjugate Induces Glycosylation-Dependent Cancer Cell–Specific Apoptosis
Authors: Lee Sok-Hyong , Khwaja Rehman Fatima , Tyler Kari C. , Yu Bing , Zhang Zhaobin , Osuka Satoru , Zerrouqi Abdessamad , Kaluzova Milota , Hadjipanayis Costas G. , Cummings Richard D. , Olson Jeffrey J. , Devi Narra S. , Van Meir Erwin G. 
Published: April 2023

<p>Table of contents: 1.	Abbreviations 2.	Author Contributions 3.	Extended Material and Methods 4.	Supplementary Figure Legends 5.	Supplementary References</p>
Effects of different joint wall lengths on in-plane compression properties of 3D braided jute/epoxy composite honeycombs
Authors: Qian-Qian Li , Mosleh Yasmine , Alderliesten RC , Hong-Hua Zhang , Wei Li 
Published: April 2023

To comply with the trend in the development of engineering materials towards lightweight, high strength, eco-friendly, sustainable, and multi-functional, a three-dimensional braided integrated composite honeycomb is designed. The effects of geometrical parameters particularly joint wall lengths on the in-plane mechanical behavior of the honeycombs were investigated. The results show that the in-plane mechanical properties are related to the number of cell walls, and the angle between the cell wall and the loading direction. Increasing the number of cell rows to double and triple at similar areal density lead to an improvement of the maximum load up to 2.5, and 3.8 times, respectively. Similarly, the total absorbed strain energy increased up to 2.6 and 5.9 times, respectively. The displacement at the maximum load is increased by 1.6 and 2.7 times as a result of increasing the cell row number. The total absorbed strain energy increased to 1.7 and 1.3 times, respectively. The failure angle of the 3D braided composite honeycomb is about 4°–7°. This investigation presents the geometrical factors of a 3D braided composite honeycomb can be further designed and optimized, but it also provides a reference for the development and design of a new composite honeycomb.
Action Mechanism of Extracts from Cerasus serrulata Flower for Hair-Blacking in C57BL/6 Mice
Authors: Zhang Bing , Zhang Wenyun , Luo Jianfang , He Jian , Rong Baoshan , Zheng Xiaomin , Zhu Siyang , Xu Xian , Ai Yong , Zhang Lanyue , He Tinggang 
Published: April 2023

Background Hair color retention and rejuvenation have recently emerged as a new research hot spot, with many studies focused on developing treatments to prevent premature greying. Aim This study is aimed to explore the hair-blacking effects and possible mechanisms of Cerasus serrulata flower extracts (CSE) using a C57BL/6 premature greying mouse model. Materials and Methods The variations in hair follicle number, hair follicle depth and cortical thickness were evaluated by hematoxylin and eosin staining. The quantity changes of melanocytes were also evaluated using Masson-Fontana Melanin staining. The relative abundance of vascular endothelial growth factor (VEGF), β-catenin and tyrosinase transcripts following transformed growth were further quantified using RT-qPCR and immunofluorescence. The changes in protein level expression of β-catenin, VEGF and tyrosinase were also evaluated by western blot. Results The β-catenin and VEGF expression were significantly up-regulated in skin tissues treated with C. serrulata extracts, promoting hair growth and blackening. Conclusion CSE can promote the efficient hair-blacking likely via the VEGF pathway, making it a potential therapeutic candidate for treating premature greying.
Regional and seasonal variations in functional abdominal pain and functional constipation prevalence among Saudi children
Authors: Khayat Ammar , Aldharman Sarah Salem , Alharbi Njoud Naif , Alayyaf Abdulaziz Saad , abdulmuttalib Jannat Abdullah , Altalhi Elaf Rudda 
Published: April 2023

Objectives: This study aimed to evaluate functional abdominal pain disorders and functional constipation prevalence in the central region of Saudi Arabia, and compare it to that of the western region. Methods: This was a cross-sectional study using online questionnaires targeting the general population of Riyadh region of Saudi Arabia. Subjects were randomly selected by sharing links on social media groups. Any parent with a 3–18-year-old child was included, and children with chronic medical illnesses or symptoms of organic GI disorders were excluded. Results: Three hundred nineteen subjects were included in the final analysis; the prevalence of functional abdominal pain disorders overall was 6.2% and the prevalence of functional constipation was 8.1%. Conclusions: Functional constipation diagnosis seems to be affected by life stressors or a previous viral illness. Seasonal variations had minimal effect on functional abdominal pain disorder and functional constipation symptom frequency and severity.
The Effect of Design and Size of the Fluid-Bed Equipment on the Particle Size-Dependent Trend of Particle Coating Thickness and Drug Prolonged-Release Profile
Authors: Brezovar Teja , Hudovornik Grega , Perpar Matjaž , Luštrik Matevž , Dreu Rok 
Published: April 2023

AbstractThe focus of the current work is to study and demonstrate the impact of the design, the scale, and settings of fluid-bed coating equipment on the differences in pellet coating thickness, which in case of prolonged-release pellets dictates the drug release. In the first set of coating experiments, the pellet cores were coated with the Tartrazine dye with the aim of estimating the coating equipment performance in terms of coating thickness distribution, assessed through color hue. In the second set, drug-layered pellets were film-coated with prolonged-release coating and dissolution profile tests were performed to estimate the thickness and uniformity of the coating thickness among differently sized pellets. In both study parts, film coating was performed at the laboratory and the pilot scale and essentially two types of distribution plate and different height adjustments of the draft tube were compared. The dye coating study proved to be extremely useful, as the results enable process correction and the optimal use of the process equipment in combination with the appropriate process parameters. Preferential film coating of larger drug-containing pellets was confirmed on the laboratory scale, while on the pilot scale, it was possible to achieve preferential coating of smaller pellets using rational alternatives of settings, which is desirable in terms of particle size-independent drug release profile of such prolonged-release dosage forms.
Quality use of research evidence: practitioner perspectives
Authors: Gleeson Joanne , Rickinson Mark , Walsh Lucas , Cutler Blake , Salisbury Mandy , Hall Genevieve , Khong Hang 
Published: April 2023

Background:
This article comes in response to two gaps within the research use literature: a lack of work on quality of use as distinct from quality of evidence, and a lack of research use models based on practitioner, as opposed to researcher, perspectives.
Aims and objectives:
The study probes into the views of education practitioners about ‘using research well’, and explores: (1) the extent to which those views align with or differ from a conceptual framework of quality research use; and (2) whether and how practitioner views can provide deeper insights into quality use of research in practice.
Methods:
The article draws on open-text survey (n=492) and interview (n=27) responses from Australian teachers and school leaders, which were analysed in relation to components of the Quality Use of Research Evidence (QURE) Framework.
Findings:
There was considerable alignment between the practitioners’ views and the QURE Framework, but greater recognition for certain enablers such as ‘skillsets’ and ‘leadership’, as compared with others, such as ‘relationships’ and ‘infrastructure’. The practitioners’ accounts provided nuanced descriptions and elaborations of different aspects of using research well.
Discussion and conclusions:
The findings suggest that: the QURE Framework has empirical validity as a way of conceptualising quality research use; practitioners’ views on using research well can inform future capacity building efforts; and research use as a field needs far more work that is focused on the quality of use and the perspectives of users.
Steroid Use in the NICU: Treatment and Tapering
Authors: McNerney Kyle P. , Arbeláez Ana Maria 
Published: April 2023

AbstractThe adrenal gland cortex produces life-sustaining steroid hormones that are critical for the development and survival of the fetus and neonate. Antenatal and postnatal administration of steroids has critical therapeutic effects in preterm infants. However, prolonged postnatal steroid therapy for more than 1 to 2 weeks is associated with iatrogenic adrenal insufficiency and should prompt consideration of a steroid taper and stress dose precautions. In this review, we will describe fetal adrenal development and steroidogenesis, the effect of antenatal exogenous steroids, the therapeutic role of postnatal steroids, evaluation and treatment of adrenal insufficiency, and the role of steroid tapers after prolonged steroid treatment.
A Scalable Radiomics- and Natural Language Processing–Based Machine Learning Pipeline to Distinguish Between Painful and Painless Thoracic Spinal Bone Metastases: Retrospective Algorithm Development and Validation Study
Authors: Naseri Hossein , Skamene Sonia , Tolba Marwan , Faye Mame Daro , Ramia Paul , Khriguian Julia , David Marc , Kildea John 
Published: April 2023

Background
The identification of objective pain biomarkers can contribute to an improved understanding of pain, as well as its prognosis and better management. Hence, it has the potential to improve the quality of life of patients with cancer. Artificial intelligence can aid in the extraction of objective pain biomarkers for patients with cancer with bone metastases (BMs).

Objective
This study aimed to develop and evaluate a scalable natural language processing (NLP)– and radiomics-based machine learning pipeline to differentiate between painless and painful BM lesions in simulation computed tomography (CT) images using imaging features (biomarkers) extracted from lesion center point–based regions of interest (ROIs).

Methods
Patients treated at our comprehensive cancer center who received palliative radiotherapy for thoracic spine BM between January 2016 and September 2019 were included in this retrospective study. Physician-reported pain scores were extracted automatically from radiation oncology consultation notes using an NLP pipeline. BM center points were manually pinpointed on CT images by radiation oncologists. Nested ROIs with various diameters were automatically delineated around these expert-identified BM center points, and radiomics features were extracted from each ROI. Synthetic Minority Oversampling Technique resampling, the Least Absolute Shrinkage And Selection Operator feature selection method, and various machine learning classifiers were evaluated using precision, recall, F1-score, and area under the receiver operating characteristic curve.

Results
Radiation therapy consultation notes and simulation CT images of 176 patients (mean age 66, SD 14 years; 95 males) with thoracic spine BM were included in this study. After BM center point identification, 107 radiomics features were extracted from each spherical ROI using pyradiomics. Data were divided into 70% and 30% training and hold-out test sets, respectively. In the test set, the accuracy, sensitivity, specificity, and area under the receiver operating characteristic curve of our best performing model (neural network classifier on an ensemble ROI) were 0.82 (132/163), 0.59 (16/27), 0.85 (116/136), and 0.83, respectively.

Conclusions
Our NLP- and radiomics-based machine learning pipeline was successful in differentiating between painful and painless BM lesions. It is intrinsically scalable by using NLP to extract pain scores from clinical notes and by requiring only center points to identify BM lesions in CT images.
Factors related to successful aging among community-dwelling elderly in Wenzhou, China
Authors: Gu Chen-Yu , Jullamate Pornchai , Nadarajan Sivasankari , Krungkraipetch Nisakorn , Toonsiri Chanandchidadussadee 
Published: April 2023

Abstract

Objectives: To examine the level of successful aging and the relationship between successful aging and activities of daily living (ADL), life satisfaction, social support, income, and self-efficacy among community-dwelling elderly in Wenzhou, China.

Methods: A descriptive correlational design was applied. Subjects were community-dwelling elderly in 4 districts of Wenzhou, China. Simple random sampling was used to recruit 83 participants. All participants were screened using the Mini-Cog tool. Data were collected using 6 questionnaires such as a demographic questionnaire, the Successful Aging Inventory (SAI), the Barthel Index for ADL, the Satisfaction With Life Scale (SWLS), the Social Support Rating Scale (SSRS), and the General Self-Efficacy Scale (GSES). Data were analyzed using descriptive statistics and Pearson correlation analysis.

Results: The results showed that 53% of the participants had a higher level of successful aging and 43.4% had a moderate level of successful aging with a mean score of 56.76 (standard deviation [SD] = 12.31). Factors such as elderly income (r = 0.73, P < 0.001) and self-efficacy (r = 0.72, P < 0.001) had high correlations with successful aging, whereas ADL (r = 0.67, P < 0.001), life satisfaction (r = 0.63, P < 0.001), and social support (r = 0.36, P < 0.001) had moderate correlations with successful aging.

Conclusions: There was a significant positive correlation between successful aging and ADL, life satisfaction, social support, income, and self-efficacy among community-dwelling elderly in Wenzhou, China. The findings may guide the formulation of more effective health education and intervention measures to improve successful aging among the elderly.
Dermatology nurse prescribing in China: a Delphi method
Authors: Han Shi-Fan , Gao Jin-Ping , Cao Yan , Zhu Rui-Fang , Wang Ya-Ping 
Published: April 2023

Abstract

Objectives: To identify the dermatological system drugs that may be prescribed by Chinese dermatological nurses through expert consensus.

Methods: A 2-round study, consisting of 32 medical and nursing experts, was conducted using the Delphi method from September 2019 to June 2020. Microsoft Excel 2019 and IBM SPSS Ver. 22 were used to analyze the results of the consultations.

Results: A total of 63 drugs across 13 categories were identified as relevant to Chinese nurses working in dermatological departments. Among these drugs, 1 drug was generally prescribed independently, 17 drugs tended to be prescribed collaboratively, and 45 were prescribed either independently or in collaboration with others.

Conclusions: This expert consensus determines the prescription drugs that may be prescribed by dermatology nurses in China, which can be used as the key content of prescription drug training for dermatology nurses in future. The results of the study could provide a basis for the implementation of nurses’ prescription rights in China in future and provide a reference for the formulation of relevant legislation on nurses’ prescription rights.
Elbow Injuries Among MLB Pitchers Increased During Covid-19 Disrupted Season, But Not Other Baseball Injuries
Authors: Martin Chelsea , Palasiewicz Jozef , Grullon Jesse , Shanley Ellen , Thigpen Charles , Kline Daniel , Kluzek Stefan , Collins Gary , Bullock Garrett 
Published: April 2023

Background The 2020 Major League Baseball Season (MLB) demonstrated season disruptions due to the COVID-19 pandemic. Changes in training and seasonal time frames may be associated with higher rates of injury. Purpose To use publicly available data to compare injury rates during the 2015-2019 seasons, COVID-19 shortened season (2020), and the 2021 season stratified by body region and position (pitchers versus position players). Study Design A retrospective cohort study utilizing publicly available data Methods MLB players who competed in 1+ seasons between 2015-2021 were included and stratified by position (pitcher, position player). Incidence rate (IR), reported by 1000 x Athlete-Game Exposures (AGEs), was calculated for each season, and stratified by position and body region. Poisson regressions were performed for all injuries and stratified by position to determine association between season and injury incidence. Subgroup analyses were performed on the elbow, groin/hip/thigh, shoulder. Results Four thousand, two hundred and seventy-four injuries and 796,502 AGEs across 15,152 players were documented. Overall IR was similar across seasons (2015-2019:5.39; 2020:5.85; 2021:5.04 per 1000 AGEs). IR remained high for the groin/hip/thigh for position players (2015-2019:1.7; 2020:2.0; 2021:1.7 per 1000 AGEs). There was no difference in injury rates between 2015-2019 and 2020 seasons [1.1 (0.9-1.2), p=0.310]. The 2020 season demonstrated a significant increase in elbow injuries [2.7 (1.8-4.0), p<0.001]; when stratified by position, this increase remained significant for pitchers [pitchers: 3.5 (2.1-5.9), p<0.001; position players: 1.8 (0.9-3.6), p=0.073]. No other differences were observed. Conclusion The groin/hip/thigh demonstrated the highest IR in 2020 among position players across all season time frames, indicating that continued injury mitigation for this region is necessary. When stratified by body region, elbow injury rates among pitchers demonstrated 3.5 times the rate of injury in 2020 compared to previous seasons, impacting injury burden for the most vulnerable body region among pitchers. Level of Evidence Level III