bluuebunny/crossref_metadata_2025_split huggingface dataset:
Komunikasi Politik Patronase NU Dalam Pilkada Kabupaten Purbalingga 2020
Authors: Nurdiyansyah Muhammad 
Published: March 2023

Nahdatul Ulama (NU) sebagai organisasi Islam, mampu membentuk patronase dengan jumlah jamaah terbesar di Kabupaten Purbalingga. Penelitian ini menganalisis pengaruhnya terhadap kecenderungan memilih masyarakat pada Pilkada Purbalingga2020, dan kriteria seperti apa yang tepat untuk Patron NU agar dapat mempengaruhi kecenderungan memilih masyarakat terhadap kandidat tertentu. Penelitian ini menggunakan metode kuantitatif dengan desain survei dan sampel 440 pemilih di Kabupaten Purbalingga. Penentuan sampel menggunakan teknik multistage random sampling dan data dianalisis mengunakan analisis CART (Classification and Regression Trees). Terdapat 7 dari 17 peubah independen yang menjadi penciri utama terpilihnya kandidat pasangan calon bupati dan wakil bupati Kabupaten Purbalingga. Berdasarkan analisis CART masyarakat Purbalingga menganggap tindakan tidak wajar ketika tokoh NU yang berpolitik mendukung secara langsung kandidat yang diusung partai PKB. Adapun tokoh NU yang harus dijaring untuk mengumpulkan suara kandidat bupati yang diusung partai PKB di antaranya yang berasal dari golongan pemuda; santri, guru non formal; perangkat desa; perangkat daerah kabupaten; karyawan; buruh; organisasi masyarakat dan partai politik yang dinilai efektif untuk menyampaikan pesan politik dalam kurun waktu beberapa kali selama sebulan ke masyarakat. Pesan politik dapat berupa penyampaian visi-misi, pengalaman, serta prestasi kandidat yang disampaikan secara alim, luwes, dan serius. Selain itu, tokoh NU juga harus memiliki keterkaitan serta rekam jejak sikap yang responsif dan solutif dalam menjawab berbagai persoalan masyarakat, agar keterlibatannya dalam berpolitik dianggap sangat wajar oleh masyakat.

Kata kunci: Patronase, NU, Pilkada Purbalingga 2020
The Effect of Counseling Depending on the Tv Watching Frequency of Children on Their Emotional and Behavioral Problems
Authors: KARAKURT Nurgül , EKİNCİ Mine 
Published: March 2023

Objective: While television continues to exist in many homes, it is thought that the situation of children being affected by the frequency of watching television is an important issue that is overlooked. This study was conducted in order to determine the effect of counselling given to parents and teachers depending on the TV watching frequency of children going to pre-school education institutions on their emotional behavioural problems.
Methods: The study was conducted as an experimental study with pre-test – post-test control group. Data were collected by using ‘Family Introduction Form’, and ‘Strengths and Difficulties Questionnaire’. Data were analysed using SPSS 20.0.
Results: The study found significant differences between the pre-test and post-test mean scores of the families and teachers in the experimental group and control group for the total score of the Strengths and Difficulties Questionnaire and the mean scores of all subscales, as well as significant difference between the pre-test and post-test mean scores in the experimental group for intra-group analysis (p< .05).
Conclusion: The study found that the counselling training given to parents and teachers lead to a significantly positive change in the mean scores for emotional, behavioural, and peer problems as well as attention deficit and hyperactivity disorders and it also has a significant positive impact on the TV watching duration of children.
Clinical application of metagenomic next-generation sequencing in tuberculosis diagnosis
Authors: Liu Ying , Wang Huifen , Li Yaoguang , Yu Zujiang 
Published: March 2023

ObjectiveThe purpose of this study was to evaluate the clinical diagnostic value of metagenomic next-generation sequencing (mNGS) for tuberculosis (TB).MethodsThis retrospective study included 52 patients with suspected TB infection. mNGS, targeted PCR, acid-fast staining and, T-SPOT.TB assay were performed on the specimen. The positive rate of mNGS and traditional detection methods was statistically analyzed. Pathological tests were performed when necessary.ResultsIn total, 52 patients with suspected of TB in this study were included in the analysis, and 31 patients were finally diagnosed with TB. Among 52 patients, 14 (26.9%) cases were positive for acid-fast staining. The positive rate of T-SPOT.TB assay in 52 patients was 73.1% (38/52). Among 52 patients, 39 (75%) were detected positive for Mycobacterium tuberculosis (MTB) by mNGS. Regarding the detection rate of MTB, mNGS were as high as 75% (39/52), whereas acid-resistant staining was only 26.9% (14/52), which showed a statistically significant difference (p&lt;0.05). The positive rates of T-SPOT.TB assay and mNGS were not statistically significant (p&gt;0.05). Of the 52 suspected TB patients, 24 had targeted PCR, of which 18 were PCR positive. In 24 patients, the positive rate of PCR was 75%, and the positive rate of mNGS was 100%, with statistical difference between them (p&lt;0.05).ConclusionsThe detection rate of MTB by mNGS was higher than that by conventional acid-fast staining and PCR, but not statistically significant compared with T-SPOT.TB assay. As an adjunctive diagnostic technology, mNGS can be combined with traditional detection methods to play a guiding role in the diagnosis and treatment of TB.
TIMP3 induces gene expression partly through PI3K and their association with vascularization and heart rate
Authors: Xia Zi-Meng , Song Meng-Yu , Chen Yan-Ling , Cui Guozhen , Fan Dong 
Published: March 2023

BackgroundTissue inhibitor of metalloproteinase 3 (TIMP3) was recently demonstrated capable to regulate some gene expression in a myocardial infarction model. Here we aim to explore the gene expression profile in TIMP3-treated cardiomyocytes and related potential cardiovascular functions.MethodsTotal RNA extracted from cultured neonatal rat ventricular myocytes (NRVMs) were used for RNA sequencing analysis and real-time PCR. KEGG pathway enrichment assay and Ingenuity Pathway Analysis (IPA) were performed to study the signaling pathways and downstream effects. Western blot was used to detect phosphorylation of protein kinase B (Akt). A Cell Counting Kit-8 assay was employed to evaluate the proliferation of human umbilical vein endothelial cells (HUVECs). Contraction rate of NRVMs was measured with microscopy.ResultsRNA sequencing data showed that expression of 2,526 genes were significantly modulated by recombinant TIMP3 (rTIMP3, 100 ng/ml) in NRVMs. Some differentially expressed genes (DEGs) were validated with real-time PCR. Several KEGG pathways including the phosphoinositide-3-kinase (PI3K)-Akt pathway were significantly regulated by rTIMP3. Phosphorylation of Akt was increased by rTIMP3 and a PI3K inhibitor LY294002 suppressed rTIMP3-induced up-regulation of some genes. Some DEGs were predicted by IPA to increase vascularization, and some to decrease heart rate. RTIMP3 could reduce the contraction rate of NRVMs and its conditioned media increased the proliferation of HUVECs.ConclusionTIMP3 can regulate expression of multiple genes partly through PI3K. Some DEGs were associated with activation of vascularization and some with heart rate reduction. This study suggests that TIMP3 can potentially modulate cardiovascular functions via DEGs.
Late Pleistocene sedimentary environment reconstruction and evolution in the Houtao Plain
Authors: Li Fuqiang , Pang Hongli , Gao Hongshan 
Published: March 2023

Reconstructing the Late Pleistocene sedimentary environment history of the Houtao Plain has great significance not only for revealing the evolution of the Yellow River but also for identifying the formation of paleolake and the northern Ulan Buh Desert. This paper presents the results of the sedimentary facies framework based on a grain-size multi-parameter analysis optically stimulated luminescence and 14C dating for a drilling core DKZ06 with a length of 20.04 m from the Houtao Plain. Grain-size multi-parameter analysis was combined with the grain size of modern environmental sediments to the division of the core sedimentary facies in this area. Sedimentary facies of core DKZ06 indicate that the formation of the Houtao Plain area is dominated by fluvial channel deposition and river changed mainly by frequent fluvial channel since ∼35 ka. The two episodes of shallow lake deposition from a drilling core, suggesting that there will not be a mega-paleolake in the Houtao Plain, and the frequent channel migration of the Yellow River may have caused a series of furiotile lakes during the Late Pleistocene. The appearance of aeolian sand at the top of core DKZ06 combined with previous stratigraphic records from the adjacent areas indicates that the origin of the northern Ulan Buh Desert is relatively late and it formed only 2,000 years ago. We propose that a combination of river migration and large-scale human activity may be responsible for desert formation. The evolution of sedimentary environments has a close correlation with climate change during the Late Pleistocene. During the interglacial stage (MIS3), the sedimentary environment has a particular lacustrine and fluvial–lacustrine environment because of relatively warm–humid conditions. During the glacial stage (MIS2), the Houtao Plain was dominated by a fluvial sedimentary environment because of a relatively cold and dry climate. The sedimentary environment is complex and changeable during the Holocene (MIS1), which was likely due to the frequent climate fluctuation.
Research on the hydraulic support top beam based on dynamic load bearing
Authors: Cao Lianmin , Jin Xin , Qin Jianli , Zhang Xiaowei , Gao Lili 
Published: March 2023

Introduction: Aiming at the problem of structural damage of top beam and column in the actual bearing process of top coal caving hydraulic support caused by uneven roof pressure in the process of fully mechanized caving mining in thick coal seam coal mine, this paper analyzes the dynamic bearing performance of ZF20000/26/48 top coal caving hydraulic support based on the working conditions given by the 101 working face of Shaanxi Zheng-tong Coal Mine.Methods: Genetic algorithm was used to optimize the top beam of hydraulic support.Results: Under the same loading conditions, the maximum stress of the optimized support is reduced by 22.28MPa, and the stress of the top beam is more uniform, and the maximum stress is reduced by 24.53MPa.Discussion: The purpose of optimization design is achieved, and the bearing performance of hydraulic support is improved,A new method using genetic algorithm to optimize the top beam of hydraulic support is developed.
Obesity mechanism after hypothalamic damage: Cohort analysis of neuroimaging, psychological, cognitive, and clinical phenotyping data
Authors: Lee Miwoo , Park Min-Jung , Lee Kyung Hwa , Kim Jung Hee , Choi Hyung Jin , Kim Yong Hwy 
Published: March 2023

ObjectiveThe hypothalamus regulates energy homeostasis, and its damage results in severe obesity. We aimed to investigate the multifaceted characteristics of hypothalamic obesity.MethodsWe performed multidimensional analyses of brain structure/function and psychological and behavioral phenotypes in 29 patients with hypothalamic damage (HD) (craniopharyngioma) and 31 controls (non-functional pituitary adenoma). Patients underwent structural and functional magnetic resonance imaging and completed self-reports and cognitive tasks.ResultsPatients with HD showed significantly higher postoperative weight gain than controls. The HD group also showed significant hypothalamic damage and lower neural activation in the left caudate nucleus in response to food images. The HD group had significantly higher food inattention, lower satiety, and higher restrained eating behavior. Within the HD group, higher restrained eating behavior was significantly associated with lower activation in the bilateral fusiform gyrus.ConclusionThese results suggest that hypothalamic damage contributes to weight gain by altering the brain response, attention, satiety, and eating behaviors. The present study proposes novel neuro-psycho-behavioral mechanisms targeted for patients with hypothalamic obesity.
A comparison of five models in predicting surface dead fine fuel moisture content of typical forests in Northeast China
Authors: Fan Jiale , Hu Tongxin , Ren Jinsong , Liu Qi , Sun Long 
Published: March 2023

IntroductionThe spread and development of wildfires are deeply affected by the fine fuel moisture content (FFMC), which is a key factor in fire risk assessment. At present, there are many new prediction methods based on machine learning, but few people pay attention to their comparison with traditional models, which leads to some limitations in the application of machine learning in predicting FFMC.MethodsTherefore, we made long-term field observations of surface dead FFMC by half-hour time steps of four typical forests in Northeast China, analyzed the dynamic change in FFMC and its driving factors. Five different prediction models were built, and their performances were compared.ResultsBy and large, our results showed that the semi-physical models (Nelson method, MAE from 0.566 to 1.332; Simard method, MAE from 0.457 to 1.250) perform best, the machine learning models (Random Forest model, MAE from 1.666 to 1.933; generalized additive model, MAE from 2.534 to 4.485) perform slightly worse, and the Linear regression model (MAE from 2.798 to 5.048) performs worst.DiscussionThe Simard method, Nelson method and Random Forest model showed great performance, their MAE and RMSE are almost all less than 2%. In addition, it also suggested that machine learning models can also accurately predict FFMC, and they have great potential because it can introduce new variables and data in future to continuously develop. This study provides a basis for the selection and development of FFMC prediction in the future.
Larval and adult diet affect phenotypic plasticity in thermal tolerance of the marula fly, Ceratitis cosyra (Walker) (Diptera: Tephritidae)
Authors: Pullock Dylan A. , Malod Kévin , Manrakhan Aruna , Weldon Christopher W. 
Published: March 2023

IntroductionTemperature fluctuations are important for the distribution and survival of insects. Rapid hardening, a type of phenotypic plasticity, is an adaptation that can help individuals better tolerate lethal temperatures because of earlier exposure to a sublethal but stressful temperature. Nutrition and sex are also known to influence a species ability to tolerate thermal stress. This study determined the effects of larval diet, adult diet, sex and hardening on the thermal tolerance of Ceratitis cosyra (Walker) (Diptera: Tephritidae) at lower and upper lethal temperatures.MethodsLarvae were raised on either an 8% torula yeast (high) or a 1% torula yeast (low) larval diet and then introduced to one of three dietary regimes as adults for thermal tolerance and hardening assays: no adult diet, sugar only, or sugar and hydrolysed yeast diet. Flies of known weight were then either heat- or cold-hardened for 2 hours before being exposed to a potentially lethal high or low temperature, respectively.ResultsBoth nutrition and hardening as well as their interaction affected C. cosyra tolerance of stressful temperatures. However, this interaction was dependent on the type of stress, with nutrient restriction and possible adult dietary compensation resulting in improved cold temperature resistance only.DiscussionThe ability of the insect to both compensate for a low protein larval diet and undergo rapid cold hardening after a brief exposure to sublethal cold temperatures even when both the larva and the subsequent adult fed on low protein diets indicates that C. cosyra have a better chance of survival in environments with extreme temperature variability, particularly at low temperatures. However, there appears to be limitations to the ability of C. cosyra to cold harden and the species may be more at risk from long term chronic effects than from any exposure to acute thermal stress.
Machine learning reveals sex differences in clinical features of acute exacerbation of chronic obstructive pulmonary disease: A multicenter cross-sectional study
Authors: Chen Zhihong , Wang Jiajia , Wang Hanchao , Yao Yu , Deng Huojin , Peng Junnan , Li Xinglong , Wang Zhongruo , Chen Xingru , Xiong Wei , Wang Qin , Zhu Tao 
Published: March 2023

IntroductionIntrinsically, chronic obstructive pulmonary disease (COPD) is a highly heterogonous disease. Several sex differences in COPD, such as risk factors and prevalence, were identified. However, sex differences in clinical features of acute exacerbation chronic obstructive pulmonary disease (AECOPD) were not well explored. Machine learning showed a promising role in medical practice, including diagnosis prediction and classification. Then, sex differences in clinical manifestations of AECOPD were explored by machine learning approaches in this study.MethodsIn this cross-sectional study, 278 male patients and 81 female patients hospitalized with AECOPD were included. Baseline characteristics, clinical symptoms, and laboratory parameters were analyzed. The K-prototype algorithm was used to explore the degree of sex differences. Binary logistic regression, random forest, and XGBoost models were performed to identify sex-associated clinical manifestations in AECOPD. Nomogram and its associated curves were established to visualize and validate binary logistic regression.ResultsThe predictive accuracy of sex was 83.930% using the k-prototype algorithm. Binary logistic regression revealed that eight variables were independently associated with sex in AECOPD, which was visualized by using a nomogram. The AUC of the ROC curve was 0.945. The DCA curve showed that the nomogram had more clinical benefits, with thresholds from 0.02 to 0.99. The top 15 sex-associated important variables were identified by random forest and XGBoost, respectively. Subsequently, seven clinical features, including smoking, biomass fuel exposure, GOLD stages, PaO2, serum potassium, serum calcium, and blood urea nitrogen (BUN), were concurrently identified by three models. However, CAD was not identified by machine learning models.ConclusionsOverall, our results support that the clinical features differ markedly by sex in AECOPD. Male patients presented worse lung function and oxygenation, less biomass fuel exposure, more smoking, renal dysfunction, and hyperkalemia than female patients with AECOPD. Furthermore, our results also suggest that machine learning is a promising and powerful tool in clinical decision-making.
Correlation between cognitive impairment and serum phosphorylated tau181 protein in patients with preeclampsia
Authors: Wang Yuanyuan , Guo Bin , Zhao Ke , Yang Linfeng , Chen Tao 
Published: March 2023

ObjectiveTo study the cognitive function status, serum phosphorylated tau181 (P-tau181) protein level, and total tau (T-tau) protein level in patients with preeclampsia (PE), pregnant healthy controls (PHCs), and non-pregnant healthy controls (NPHCs), and to research their feasibility as serum biomarkers for evaluating cognitive functional impairment in PE patients.MethodsSixty-eight patients with PE, 48 NPHCs, and 30 PHCs were included. Cognitive functional status was assessed using standardized Symbol Digit Modalities Test (SDMT) and Montreal Cognitive Assessment (MoCA) scales. Enzyme-linked immunosorbent assay (ELISA) was used to detect the level of serum P-tau181 and T-tau protein. The concentration of serum P-tau181 and T-tau protein were compared by one-way analysis of variance in the three groups of subjects. The correlation between P-tau181, T-tau, and SDMT was explore by multiple linear regression analysis. The areas under the receiver operating characteristic (ROC) curves of serum P-tau181 and SDMT were calculated to predict the cognitive level of subjects.ResultsPE patients significantly had lower scores on SDMT (47.97 ± 7.54) and MoCA (28.00 ± 2.00) than normotensive PHCs (30.00 ± 1.25, 54.73 ± 8.55, respectively). The significant difference was found in serum P-tau181 protein levels among the three groups [H(K) = 19.101, P &lt; 0.001]. Serum P-tau181 was thicker in PE patients than PHCs or NPHCs (both P &lt; 0.05). According to the ROC curve, T-tau had no statistical significance in predicting the ability of cognizance, while P-tau181 and SDMT had. The DeLong test showed that P-tau181 was better than T-tau in predicting the ability of cognizance (P &lt; 0.05).ConclusionThe patients with PE have occurred the decline of cognitive function during pregnancy. The high level of serum P-tau181 can be used as a clinical laboratory indication for non-invasive assessment of cognitive functional impairment in PE patients.
Over-expression of RRM2 predicts adverse prognosis correlated with immune infiltrates: A potential biomarker for hepatocellular carcinoma
Authors: Qin Zhongqiang , Xie Bo , Qian Jingyu , Ma Xiang , Zhang Lan , Wei Jianzhu , Wang Zhaoying , Fan Longfei , Zhu Ziyi , Qian Zhen , Yin Hongxiang , Zhu Fangquan , Tan Yulin 
Published: March 2023

BackgroundRibonucleotide reductase regulatory subunit M2 (RRM2) has been reported to be an oncogene in some malignant tumors, such as lung adenocarcinoma, oral squamous cell carcinoma, glioblastoma, and breast cancer. However, the clinical significance of RRM2 in hepatocellular carcinoma has been less studied. The aim of this study was to assess the importance of RRM2 in hepatocellular carcinoma (HCC) based on the Cancer Genome Atlas (TCGA) database.MethodsThe RRM2 expression levels and clinical features were downloaded from the TCGA database. Immunohistochemistry results between tumor tissues and normal tissues were downloaded from the Proteinatlas database. Meanwhile, the expression levels of RRM2 in tumor and paraneoplastic tissues were further verified by qRT-PCR and Western Blotting. Gene Ontology (GO)/Kyoto Encyclopedia of Genes and Genomes (KEGG) and protein-protein-interactions (PPI) network were constructed to analyze RRM2-related downstream molecules. In addition, RRM2 expression-related pathways performed by gene set enrichment analysis (GSEA). Association analysis of RRM2 gene expression and immune infiltration was performed by single-sample GSEA (ssGSEA).ResultsThe RRM2 expression level in tumor tissues was higher than normal tissues (P &lt;0.001). The elevated expression of RRM2 in HCC was significantly correlated with T stage (P &lt;0.05), pathologic stage (P &lt;0.05), tumor status (P &lt;0.05), histologic grade (P&lt;0.001), and AFP (P &lt;0.001). HCC with higher RRM2 expression was positively associated with worse OS (overall survival), PFS (progression-free survival), and DSS (disease-specific survival). In the univariate analysis, the expression of RRM2, T stage, M stage, pathologic stage, and tumor status were negatively correlated with OS (P &lt;0.05). Further analysis using multivariate Cox regression showed that tumor status (P&lt;0.01) and RRM2 expression (P&lt;0.05) were independent prognostic factors of OS in HCC. GO/KEGG analysis showed that the critical biological process (chromosome condensation and p53 signaling pathway) might be the possible function mechanism in promoting HCC. Moreover, GSEA showed that several pathways were enriched in RRM2 high-expression samples, including PD-1 signaling, cell cycle, P27 pathway, and T cell receptor signaling pathway. RRM2 was significantly correlated with the infiltration level of CD8 T cells, Cytotoxic cells, DCs, Neutrophils, NK cells, and T helper cells (P &lt;0.05).ConclusionOver-expression of RRM2 predict adverse prognosis and is correlated with immune infiltrates in HCC. RRM2 may be a significant molecular biomarker for HCC diagnosis and prognosis.