bluuebunny/crossref_metadata_2025_split huggingface dataset:
Stepwise conversion of methane to methanol on Cu and Fe/zeolites prepared in solid state: the effect of zeolite type and activation temperature
Authors: Le Ha V , Ho Phuoc H , Trunschke Annette , Schomäcker Reinhard , Thomas Arne 
Published: February 2023

AbstractBACKGROUNDGrand efforts have been recently devoted to the development of catalysts based on the excellent performance of Cu‐ and Fe‐dependent enzymes in methanotrophic bacteria for the partial oxidation of methane to methanol under ambient conditions. As a continuation of the study on the stepwise manner for this conversion over zeolite‐based catalysts, in this work, the effects of zeolite topology and activation temperature on the catalytic performance of Cu‐ and Fe‐containing zeolites were investigated.RESULTSCu species exchanged in the medium‐pore zeolites (mordenite, ZSM‐5, and ferrierite) afforded better methanol production, while large‐pore zeolites (zeolite β and zeolite γ) were inappropriate to accommodate active Cu sites. Notably, Cu/silicalite‐1 containing CuO species was also reactive to methane after the activation in O2, yielding a minor methanol amount. Furthermore, the activity of Fe/mordenite towards the methanol in the O2‐assisted procedure was reported for the first time but with a much lower yield as compared to that of Cu/mordenite. The methanol yield over Cu/mordenite increased with the activation temperature because increasing the activation temperature favored the Cu‐exchange degree with a higher priority at the side pockets as compared to the main channels of mordenite, as evidenced from infrared analysis.CONCLUSIONThe selective oxidation of methane to methanol by O2 via a stepwise manner can be obtained over both ion‐exchanged Cu species and well‐dispersed CuO nanoparticles with better activity being recorded for the former. The activity of Cu‐exchanged zeolites was considerably dependent on the zeolite topology and the charge‐balancing position of the Cu2+ cations. © 2023 Society of Chemical Industry (SCI).
Maternal hyperoxygenation during pregnancy as a tool in fetal disease diagnosis and treatment
Authors: Zalinska Agnieszka , Respondek‐Liberska Maria , Slodki Maciej 
Published: February 2023

AbstractMaternal hyperoxygenation (MHO) consists of giving pregnant women (60% to 100%) oxygen through a facemask and using ultrasound assess or monitor the influence on fetal cardiovascular circulation. This review discusses the findings and the utility of acute and chronic MHO in various fetal diseases.
Fetal cardiac function evaluation: A review
Authors: Vasciaveo Lorenzo , Zanzarelli Erika , D'Antonio Francesco 
Published: February 2023

AbstractThe aim of this review is to provide an up to date on the current use of fetal echocardiography in assessing the fetal cardiac function and its potential research and clinical applications. Despite classically is been used for prenatal diagnosis of fetal heart defects, assessment of fetal cardiac function has been recently proposed as a fundamental tool to assess pregnancies complicated by several disorders with long‐term impact on post‐natal cardiovascular health, such as placental insufficiency and fetal growth restriction. In this review we present anatomical and functional fetal cardiac development mechanisms and an overview of the currently available techniques for evaluating fetal heart function.
Jaw morphology and function in Drilonereis cf. filum (Oenonidae, Annelida)
Authors: Tzetlin Alexander , Vortsepneva Elena , Zhadan Anna 
Published: February 2023

AbstractRepresentatives of the extant family Oenonidae (Annelida, Eunicida) have a prionognath jaw apparatus, with maxillae having forceps‐like elements, a number of asymmetrical dentate plates and long slender carriers, which is characteristic of some fossil forms known from the Paleozoic epoch. Therefore, data on the fine structure and functional morphology of Oenonidae jaws are helpful for the interpretation of fossil materials. The fine structure of the jaw apparatus and the ventral pharyngeal organ is studied in one species of the Oenonidae (Annelida)—Drilonereis cf. filum. The material was collected in the soft bottom of Marseille Bay (Mediterranean) and examined with the help of TEM and histological techniques. A three‐dimensional (3D) reconstruction was made from a complete series of semithin sections. The entire jaw apparatus is about 500 µm in length; it includes ventral mandibles and four pairs of maxillae, connected with long paired dorsal carriers and an unpaired ventral carrier. While retracted, it reaches the VIII−XI chaetigers. The most solid part of the maxillary apparatus, that is, maxillae I and II, are 2.5−5 µm thick. The plate consists of a monolithic array of merged scleroprotein granules in which perforations, that is, spaces remaining from microvilli, are visible; the basal part of the maxillary plate is a layer of loosely arranged collagen fibers penetrated with microvilli and has no signs of sclerotization. A study of the jaws of Drilonereis cf. filum showed the presence of common jaw patterns in Eunicida order. Like the jaws of Dorvilleidae, Eunicidae, Onuphidae, and Lumbrineridae, the jaws of Drilonereis are formed at the basis of a typical annelid cuticle's transformation with epi‐ and basicuticular layers, and its impregnation by merging scleroprotein granules. Through the nature of sclerotization, the jaws of D. cf. filum are similar to those of Dorvilleidae, Histriobdellidae, and the juvenile jaws of Mooreonuphis stigmatis (Onuphidae). Analysis of the 3D‐reconstructions of the D. cf. filum jaw apparatus shows that the MxI of this species, and probably of other Oenonidae with dorsal and ventral carriers, can make grasping motions by fixing the joint of the right and left MxI in the two‐door hinge type. In general, the overall structure of the jaw apparatus of D. cf. filum and the mechanics of its work shows greater similarity with that of Dorvilleidae than with the jaw apparatus of extant Labidognatha and Simmetrognatha (Onuphidae, Eunicidae, Lumbrineridae). The need for compactization of the jaw apparatus when moving in dense sediment or in the burrows is probably one of the factors determining its structure.
Monkeypox awareness and low vaccination hesitancy among men who have sex with men in China
Authors: Li Yuwei , Peng Xin , Fu Leiwen , Wang Bingyi , Sun Yinghui , Chen Yuanyi , Lin Yi‐Fan , Wu Xinsheng , Liu Qi , Gao Yanxiao , Zheng Weiran , Bian Junye , Lu Zhen , Meng Xiaojun , Xue Hui , Li Hui , Jiang Hongbo , Zou Huachun 
Published: February 2023

AbstractMen who have sex with men (MSM) have been recommended for targeted monkeypox vaccination. We aimed to investigate monkeypox awareness and explore the correlates of monkeypox vaccination hesitancy among MSM in China. We conducted a cross‐sectional survey from August 10 to September 9, 2022. Awareness related to monkeypox and attitude toward monkeypox vaccination among MSM aged ≥18 years were collected. Multivariable logistic regression was applied to evaluate correlates of vaccination hesitancy. The discrepancy in awareness between subgroups regarding HIV status was assessed. A total of 1090 MSM were included (age: median 30 years, interquartile range [IQR], 25–35; HIV‐infected: 53.12%). Only 13.85% of respondents expressed high monkeypox vaccination hesitancy. Hesitancy was associated with no fixed income (adjuster odds ratio [aOR], 2.46, 95% confidence interval [CI], 1.48–4.11), infrequent information following (sometimes, 3.01, 1.55–5.83; seldom or never, 5.66, 2.58–12.45), and lack of worries about monkeypox endemic (1.78, 1.11–2.87). Participants who believed that HIV‐infected cases accounted for a smaller proportion (1.62, 1.01–2.60), disagreed that monkeypox virus could be detected in semen (2.21, 1.26–3.88), and considered either replication‐competent (1.84, 1.14–2.96) or replication‐deficient (4.80, 2.26–10.21) monkeypox vaccine unsuitable for HIV‐infected people were generally more hesitant. Compared with HIV‐uninfected MSM, HIV‐infected MSM supported more for vaccination promotion. MSM in China had low hesitancy toward monkeypox vaccination. Safety and affordability of vaccine and availability of information were essential aspects to reduce hesitancy. Education on vaccination benefits should be encouraged to promote future vaccination plans.
Effect of coating with chitosan enriched with different borates on the shelf life of fish fillet
Authors: Alak Gonca , Ucar Arzu , Parlak Veysel , Turkez Hasan , Kocaman Esat Mahmut , Atamanalp Muhammed , Abd El‐Aty A. M. 
Published: February 2023

AbstractBACKGROUNDIn this study, the effects of biofilm coatings obtained by immobilization of different borates – namely borax (BX), colemanite (COL), and ulexite (UX) – with chitosan (Ch) on the shelf life of rainbow trout fillets were investigated. The immobilization and characterization of borates in Ch were confirmed by scanning electron microscopy, Fourier transform infrared spectroscopy, and zeta potential analysis. In determining the shelf life of fillets that were covered by immersion and stored for 15 days, microbiological (total aerobic mesophilic, psychrotrophic, lactic acid, Pseudomonas, and Enterobacteriaceae bacteria counts) and chemical analyses (total volatile basic nitrogen, thiobarbituric acid reactive substance, and pH levels) were performed at 3 day periodic intervals. In addition, the biodegradation of borates was determined using inductively coupled plasma mass spectrometry in biofilm‐coated fillets on the 1st, 8th, and 15th storage days.RESULTSThe microbial results of the coatings enriched with borates (BX, COL, and UX) at different levels (0, 0.03, and 0.06 mg L−1) (due to the immobilization with Ch) show the shelf life was extended by 3–6 days in all of the treatment groups compared with the control.CONCLUSIONIt was concluded that BX, COL, and UX coatings enriched by immobilization with Ch increase shelf life and improve fillet quality. In addition, the enrichment of BX, COL, and UX with Ch showed explicit natural protective effects. This study demonstrates that Ch‐enriched coatings of BX, COL, and UX can be used as natural bioactive nanocarriers to provide bioactive food ingredients in the seafood processing industry. © 2023 Society of Chemical Industry.
Phase 1 evaluation of an elastomeric nucleus pulposus device as an option to augment disc at microdiscectomy: Experimental results from biomechanical and biocompatibility testing and first in human
Authors: Chen Xiaolong , Kohan Saeed , Bhargav Divya , Choi Johnathon , Perera Senori , Dean Cameron , Chopra Neha , Sial Alisha , Sandhu Harvinder S. , Apos Esther , Appleyard Richard , Diwan Ashish D. 
Published: February 2023

AbstractObjectiveWhilst microdiscectomy is an excellent reliever of pain for recalcitrant lumbar disc herniation (LDH), it has a high failure rate over time due to the ensuing reduction in mechanical stabilization and support of the spine. One option is to clear the disc and replace it with a nonhygroscopic elastomer. Here, we present the evaluation of biomechanical and biological behavior of a novel elastomeric nucleus device (Kunovus disc device [KDD]), consisting of a silicone jacket and a two‐part in situ curing silicone polymer filler.Materials and MethodsISO 10993 and American Society for Testing and Materials (ASTM) standards were used to evaluate the biocompatibility and mechanics of KDD. Sensitization, intracutaneous reactivity, acute systemic toxicity, genotoxicity, muscle implantation study, direct contact matrix toxicity assay, and cell growth inhibition assay were performed. Fatigue test, static compression creep testing, expulsion testing, swell testing, shock testing, and aged fatigue testing were conducted to characterize the mechanical and wear behavior of the device. Cadaveric studies to develop a surgical manual and evaluate feasibility were conducted. Finally, a first‐in‐human implantation was conducted to complete the proof of principle.ResultsThe KDD demonstrated exceptional biocompatibility and biodurability. Mechanical tests showed no Barium‐containing particles in fatigue test, no fracture of nucleus in static compression creep testing, no extrusion and swelling, and no material failure in shock and aged fatigue testing. Cadaver training sessions showed that KDD was deemed implantable during microdiscectomy procedures in a minimally invasive manner. Following IRB approval, the first implantation in a human showed no intraoperative vascular and neurological complications and demonstrated feasibility. This successfully completed Phase 1 development of the device.ConclusionThe elastomeric nucleus device may mimic native disc behavior in mechanical tests, offering an effective way for treating LDH by way of Phase 2 and subsequent clinical trials or postmarket surveillance in the future.
Child posttraumatic stress symptoms in an acute injury sample: Patterns of associations among child report, parent report, and child heart rate parameters
Authors: Bailey Megan , Meiser‐Stedman Richard , Hiller Rachel , Haag Katharina , Lobo Sarah , Halligan Sarah L. 
Published: February 2023

AbstractParent–child agreement on measures of child posttraumatic stress disorder (PTSD) is moderate at best, and understanding of this discrepancy is limited. To address this, we conducted an item‐level investigation of parent–child symptom agreement to examine the potential influence of parental posttraumatic stress symptoms (PTSS) on parents’ reports of their child's PTSS. We also examined heart rate (HR) indices as possible independent indicators of child PTSD, examining patterns of association with parent versus child report. Parent–child dyads (N = 132, child age: 6–13 years, 91.7% White) were recruited after the child's hospital admission following an acute, single‐incident traumatic event. At 1‐month posttrauma, questionnaires assessing children's PTSS (self‐ and parental reports) and parental PTSS were administered. For a subset of participants (n = 70), children's HR recordings were obtained during a trauma narrative task and analyzed. Parent and child reports of child PTSS were weakly positively correlated, r = .25. Parental PTSS were found to be stronger positive predictors of parental reports of child PTSS than the children's own symptom reports, β = 0.60 vs. β = 0.14, and were associated with higher parent‐reported child PTSS relative to child reports. Finally, children's self‐reported PTSS were associated with HR indices, whereas parent reports were not, βs = −.33–.30 vs. βs = −.15–.01. Taken together, children's self‐reported PTSS could be a more accurate reflection of their posttrauma physiological distress than parent reports. The potential influence of parental PTSS on their perceptions of their child's symptoms warrants further consideration.
Rates of editor‐authored manuscripts among urology journals using blinded or non‐blinded review
Authors: Flanary James , Rengel Zachary , Sathianathen Niranjan , Lane Robert , Jarosek Stephanie , Barkve Nik , Weight Christopher 
Published: February 2023

AbstractWe compared publication rates of editor‐authored publications between journals that do not blind peer reviewers to author identity with one that does. Our hypothesis was that the if the identity of editors as authors is known to peer reviewers this may potentially bias the recommendation for publication. To do this, we queried Scopus for all publications from five top urology journals from 2013 to 2018, and linked them to a database of editors. Poisson regression analysis was used to compare publication rates of manuscripts with at least one editor as author between blinded journals and a non‐blinded journal. In separate analyses, we compared publication frequency before and after authors became editors and the frequency with which articles were cited. We found that the adjusted rate ratio of editor‐authored manuscripts comparing the non‐blinded journal to the blinded journal was 5.4 (95% CI 3.8–7.6) for ‘total publications’, and 1.9 (95% CI 1.5–2.2) among ‘articles only’. Median citation frequency was slightly higher among articles written by editors compared with non‐editors at 11 (3–26) versus 7 (2–16) (p < 0.001). We concluded that the blinded journal had a smaller representation of their editors as authors of their manuscripts, compared with the non‐blinded journals.
Carbon Quantum Dot/Chitosan‐Derived Hydrogels with Photo‐stress‐pH Multiresponsiveness for Wearable Sensors
Authors: Wei Xiaotong , Li Jie , Hu Zhirui , Wang Chen , Gao Zhiqiang , Cao Yang , Han Jing , Li Yingchun 
Published: February 2023

AbstractIn recent years, hydrogels have attracted extensive attention in smart sensing owing to their biocompatibility and high elasticity. However, it is still a challenge to develop hydrogels with excellent multiple responsiveness for smart wearable sensors. In this paper, a facile synthesis of carbon quantum dots (CQDs)‐doped cross‐linked chitosan quaternary/carboxymethylcellulose hydrogels (CCCDs) is presented. Designing of dual network hydrogels decorated with CQDs provides abundant crosslinking and improves the mechanical properties of the hydrogels. The hydrogel‐based strain sensor exhibits excellent sensitivity (gauge factor: 9.88), linearity (R2: 0.97), stretchable ability (stress: 0.67 MPa; strain: 404%), good cyclicity, and durability. The luminescent properties are endowed by the CQDs further broaden the application of hydrogels for realizing flexible electronics. More interestingly, the strain sensor based on CCCDs hydrogel demonstrates photo responsiveness (ΔR/R0≈20%) and pH responsiveness (pH range ≈4–7) performance. CCCDs hydrogels can be used for gesture recognition and light sensing switch. As a proof‐of‐concept, a smart wearable sensor is designed for monitoring human activities and detecting pH variation in human sweat during exercise. This study reveals new possibilities for further applications in wearable health monitoring.
Periostin secreted from podoplanin‐positive cancer‐associated fibroblasts promotes metastasis of gastric cancer by regulating cancer stem cells via AKT and YAP signaling pathway
Authors: Zhao Zhenxiong , Zhang Yanqiu , Guo Ergang , Zhang Yu , Wang Yanong 
Published: February 2023

AbstractCancer‐associated fibroblasts (CAFs) are heterogeneous stromal cells present in the tumor microenvironment (TME), which play a critical role in gastric cancer (GC) progression. Here, we examined a subset of CAFs with high podoplanin (PDPN) expression, which is correlated with tumor metastasis and poor survival in GC patients. Animal models of gastric cancer liver metastasis monitored by micro‐PET/CT confirmed that periostin (POSTN) derived from PDPN(+) CAFs regulated CAFs’ pro‐migratory ability. Mechanistically, PDPN(+) CAFs secreted POSTN to modulate cancer stem cells (CSCs) through FAK/AKT phosphorylation. Furthermore, POSTN could also activate FAK/YAP signaling in GC cells to produce increased amounts of IL‐6, which in turn induced phosphorylation of PI3K/AKT in PDPN(+) CAFs. Prolonged PI3K/AKT pathway activation in PDPN(+) CAFs maintains the production of POSTN and the effect on CSC enrichment and GC cell migration. In conclusion, our study demonstrated a positive feedback loop between PDPN(+) CAFs and CSCs during GC progression and suggested a selective target for GC treatment.
Stakeholder discourse coalitions and polarisation in the hen harrier conservation debate in news media
Authors: Marino Filippo , Crowley Sarah L. , Williams Foley Nell A. , McDonald Robbie A. , Hodgson Dave J. 
Published: February 2023

Abstract

Conservation conflicts are complex and can be deep‐rooted, with stakeholders holding entrenched policy positions. The actors involved producing verbal interconnected interactions that form policy debates. Thus, conservation debates can be viewed as network phenomena with stakeholders forming coalitions in support of, or opposition to, certain policies and practices.

We used Discourse Network Analysis of print media to investigate the structure and dynamics of the stakeholder debate around the management of hen harriers Circus cyaneus, a bird of prey at the centre of a long‐standing conservation conflict in the United Kingdom.

We aimed to determine whether the structure of discourse coalitions changed among the diverse aspects of the debate and whether the polarisation of the debate has changed through time. Our search and selection criteria led to the analysis of 737 statements within 131 newspaper articles published from August 1993 to December 2019.

We show that, while the discourse network of the overall debate is quite unstructured, actors formed divergent coalitions when defining the conservation problem and its solutions. In contrast, discourses converged around reactions with positive or negative emotions in relation to events and issues of hen harrier conservation. Polarisation among actors has increased over time and peaked in the second half of the 2010s, concurrent with the release of the species recovery plan.

Our study highlights the value of analysing discourse networks in conservation policy debates. Discourse networks reveal which aspects of any conservation problem cause stakeholders to converge or diverge and can identify periods of intensified debate that, ultimately, contribute to informing conflict mitigation and resolution processes.

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TUBB4B is a novel therapeutic target in non‐alcoholic fatty liver disease‐associated hepatocellular carcinoma
Authors: Yang Zhenjie , Gao Shanshan , Wong Chi Chun , Liu Weixin , Chen Huarong , Shang Haiyun , Wu Zoe Yuet , Xu Lixia , Zhang Xiang , Wong Nathalie , Kuang Ming , Yu Jun 
Published: February 2023

AbstractNon‐alcoholic fatty liver disease‐associated hepatocellular carcinoma (NAFLD‐HCC) is an emerging malignancy due to the rising prevalence of NAFLD. However, no drug is available to target NAFLD‐HCC. In this study, we aim to unravel novel therapeutic targets of NAFLD‐HCC utilizing a high‐throughput CRISPR/Cas9 screening strategy. We utilized the Epi‐drug CRISPR/Cas9 library consisting of single‐guide RNAs (sgRNAs) targeting over 1,000 genes representing the FDA‐approved drug targets and epigenetic regulators to perform loss‐of‐function screening in two NAFLD‐HCC cell lines (HKCI2 and HKCI10). CRISPR/Cas9 library screening unraveled TUBB4B as an essential gene for NAFLD‐HCC cell growth. TUBB4B was overexpressed in NAFLD‐HCC tumors compared with adjacent normal tissues (N = 17) and was associated with poor survival (p < 0.01). RNA‐sequencing and functional assays revealed that TUBB4B knockout in NAFLD‐HCC promoted cell apoptosis, cell cycle arrest, and cellular senescence, leading to suppressed NAFLD‐HCC growth in vitro and in vivo. We identified that TUBB4B inhibitor mebendazole (MBZ), an FDA‐approved drug, inhibited NAFLD‐HCC growth by inducing apoptosis and cellular senescence. Since protein expression of pro‐survival Bcl‐xL was induced in TUBB4B knockout NAFLD‐HCC cells, we examined combination of TUBB4B inhibition with navitoclax, a Bcl‐xL inhibitor that selectively targets senescent cells. Consistent with our hypothesis, either TUBB4B knockout or MBZ synergized with navitoclax to inhibit NAFLD‐HCC cell growth via the induction of intrinsic and extrinsic apoptosis pathways. In summary, TUBB4B is a novel therapeutic target in NAFLD‐HCC. Inhibition of TUBB4B with MBZ in combination with navitoclax synergistically inhibited NAFLD‐HCC cell growth, representing a promising strategy for the treatment of NAFLD‐HCC. © 2023 The Authors. The Journal of Pathology published by John Wiley & Sons Ltd on behalf of The Pathological Society of Great Britain and Ireland.