bluuebunny/crossref_metadata_2025_split huggingface dataset:
Teaching Strategies for Health Advocacy for Undergraduate Nursing Students: A Scoping Review
Authors: Gandra Elen C. , da Silva Kênia L. 
Published: January 2023

Abstract

AIM
The goal of this study was to identify strategies for teaching health advocacy for undergraduate nursing students.

BACKGROUND
Although health advocacy is a core skill of professional nursing practice, there are challenges in the health advocacy approach in nursing education.

METHOD
The databases LILACS, IBECS, BDENF, Medline, Web of Science, CINAHL, and the Cochrane Library were searched, without limit of time and language, for this scoping review; 11 articles were included. Data were analyzed and synthesized in a narrative form.

RESULTS
The following teaching strategies emerged: theoretical exposition and supervised clinical internship, technology-enhanced simulation, clinical experience, experiential learning, simulated social experiences, digital story making, and narrative pedagogy. The topic was taught according to several themes: economic disparity, health legislation, LGBTQ+ population, elder health, mental health, health policies, concept of advocacy, and nontechnical skills.

CONCLUSION
Teaching strategies for health advocacy should be based on constructivist education linked to social reality.
α-starch and glycyrrhizin denature the acrid raphides of Pinellia tuber in traditional processing
Authors: Fueki Tsukasa , Nose Itsuki , Liu Yan , Tanaka Koichiro , Namiki Takao , Makino Toshiaki 
Published: January 2023

Objective:
Pinellia tuber, the dried tuber of Pinellia ternata, causes severe acrid irritation in the mouth and throat when swallowed in its raw form. Based on the theory of traditional Chinese medicine, this acridity is reduced by heating or processing using ginger, licorice, alum, or lime. Although these detoxification methods have been known since ancient times, only little is known about the mechanisms by which they detoxify Pinellia tubers. In the present study, we aimed to reveal the effectiveness of α-starch, and glycyrrhizin in licorice for the detoxification of Pinellia tubers.

Methods:
Previously, we found that intact raphides of raw Pinellia tuber have a lipophilic character, and denaturation of the raphides reduced their lipophilicity and acridity. Accordingly, we developed a raphide denaturation assay (RDA) to quantify the degree of denaturation of raphides by measuring the absorbance of the petroleum ether (PE) layer comprising the raphides. The effect of α-starch or the decoction of licorice (the root and stolon of Glycyrrhiza uralensis) on raphide denaturation was then determined using this assay.

Results:
The treatment of raphides with α-starch markedly enhanced heat denaturation of the raphides. Licorice decoction, glycyrrhizin, and glycyrrhetinic acid significantly denatured raphides in a calcium-dependent manner at high pH. Glycyrrhizin and glycyrrhetinic acid are also attached to the denatured raphides.

Conclusions:
α-starch in Pinellia tubers contributes to detoxification by heating. In the traditional processing method for Pinellia tubers using licorice and lime, glycyrrhizin in licorice and calcium ions play important roles in denaturing raphides.

Graphical abstract:

http://links.lww.com/AHM/A42.
Therapy Strategies for Late-life Depression: A Review
Authors: Piel Carla , Quante Arnim 
Published: January 2023

Background:
Depression in the elderly requires different treatment options because therapies that are commonly used for depression in younger patients show different effects later in life. Treatment options for late-life depression (LLD) are summarized in this article.

Methods:
A literature search in Medline/PubMed performed in June 2020 identified 83 relevant studies.

Results:
Pharmacotherapy with selective serotonin reuptake inhibitors can be an effective first-line treatment in LLD, but >50% of elderly patients do not adequately respond. Switching to other selective serotonin reuptake inhibitors or augmenting with mood stabilizers or antipsychotics is often effective in achieving a therapeutic benefit. Severely depressed patients with a high risk of suicidal behavior can be treated with electroconvulsive therapy. Psychotherapy provides a measurable benefit alone and when combined with medication.

Limitations:
LLD remains an underrepresented domain in research. Paucity of data concerning the effect of specific therapies for LLD, heterogeneity in the quality of study designs, overinterpretation of results from meta-analyses, and discrepancies between study results and guideline recommendations were often noted.

Conclusions:
Treating LLD is complex, but there are several treatment options with good efficacy and tolerability. Some novel pharmaceuticals also show promise as potential antidepressants, but evidence for their efficacy and safety is still limited and based on only a few trials conducted to date.
Postpartum Depression Among Gay Fathers With Children Born Through Surrogacy: A Cross-sectional Study
Authors: Adler Limor , Yehoshua Ilan , Mizrahi Reuveni Miri 
Published: January 2023

Background:
Studies on postpartum depression (PPD) in gay fathers are scarce. The goals of this study were to examine the prevalence of PPD among Israeli gay fathers with children born through surrogacy and to identify characteristics associated with PPD in this population.

Methods:
In this descriptive cross-sectional study, we sent surveys to gay fathers who had children who were 0 to 12 months of age and were born through surrogacy. Surveys were sent through 3 major surrogacy agencies in Israel and through the media. Data were collected concerning sociodemographic, medical, and lifestyle factors as well as concerning satisfaction with partners, family, and social support. Each respondent completed the Edinburgh Postnatal Depression Scale, which screens for PPD. Characteristics of fathers with and without probable PPD were compared.

Results:
Between July 2018 and December 2019, 66 men answered our questionnaire. The respondents, mean age of 37.5 years, were mostly first-time fathers (82%), with high rates of twin pregnancies (42%). Eight respondents (12%) were classified as having depressive symptoms (95% CI: 5.4%–22.5%), and 16 respondents (25%) reported current or past use of antidepressant medications. A negative association was observed between satisfaction with family support and report of depressive symptoms (χ2
2=6.53, P=0.038) and Spearman correlation test (r
s=−0.34, P=0.005).

Conclusions:
The incidence of probable PPD among gay fathers was 12% compared with 8.8% reported in fathers in the general population. High satisfaction with family support was associated with a lower incidence of probable PPD among gay fathers. Increased awareness of the potential for PPD in gay fathers can improve early diagnosis and treatment.
Sex-dependent Differences in the Genomic Profile of Lingual Sensory Neurons in Naïve and Tongue-Tumor Bearing Mice
Authors: Ibrahim Tarek , Wu Ping , Wang Li-Ju , Fang-Mei Chang , Murillo Josue , Merlo Jaclyn , Tumanov Alexei , Lai Zhao , Weldon Korri , Chen Yidong , Ruparel Shivani 
Published: January 2023

AbstractMechanisms of sex-dependent orofacial pain are widely understudied. A significant gap in knowledge exists about comprehensive regulation of tissue-specific trigeminal sensory neurons in diseased state of both sexes. Using RNA sequencing of FACS sorted retro-labeled sensory neurons innervating tongue tissue, we determined changes in transcriptomic profiles in males and female mice under naïve as well as tongue-tumor bearing conditions Our data revealed the following interesting findings: 1) Tongue tissue of female mice was innervated with higher number of trigeminal neurons compared to males; 2) Naïve female neurons innervating the tongue exclusively expressed immune cell markers such as Csf1R, C1qa and others, that weren’t expressed in males. This was validated by Immunohistochemistry. 4) Accordingly, immune cell markers such as Csf1 exclusively sensitized TRPV1 responses in female TG neurons. 3) Male neurons were more tightly regulated than female neurons upon tumor growth and very few differentially expressed genes (DEGs) overlapped between the sexes, 5) Male DEGs contained higher number of transcription factors whereas female DEGs contained higher number of enzymes, cytokines and chemokines. Collectively, this is the first study to characterize the effect of sex as well as of tongue-tumor on global gene expression, pathways and molecular function of tongue-innervating sensory neurons.
Exploration of the Link Between COVID-19 and Alcoholic Hepatitis from the Perspective of Bioinformatics and Systems Biology
Authors: Huang Tengda , Yu Bingxuan , Zhou Xinyi , Pan Hongyuan , Du Ao , Bai Jincheng , Li Xiaoquan , Jiang Nan , He Jinyi , Yuan Kefei , Wang Zhen 
Published: January 2023

AbstractObjectiveSevere acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has been suggested to purpose threats to health of mankind. Alcoholic hepatitis (AH) is a life-threatening acute and chronic liver failure that takes place in sufferers who drink excessively. During the epidemic, AH has an increasing incidence of severe illness and mortality. However, for these two diseases, the intrinsic relationship of molecular pathogenesis, as well as common therapeutic strategies are still poorly understood.MethodsThe transcriptome of the COVID-19 and AH has been compared to obtain the altered genes and hub genes were screened out through protein-protein interaction (PPI) network analysis. Via gene ontology (GO), pathway enrichment and transcription regulator analysis, a deeper appreciation of the interplay mechanism between hub genes were established.ResultsWith 181 common differentially expressed genes (DEGs) of AH and COVID-19 were obtained, 10 hub genes were captured. Follow-up studies located that these 10 genes typically mediated the diseases occurrence by regulating the activities of the immune system. Other results suggest that the common pathways of the two ailments are enriched in regulating the function of immune cells and the release of immune molecules.ConclusionThis study reveals the common pathogenesis of COVID-19 and AH and assist to discover necessary therapeutic targets to combat the ongoing pandemic induced via SARS-CoV-2 infection and acquire promising remedy strategies for the two diseases.
Integrated ACMG approved genes and ICD codes for the translational research and precision medicine
Authors: Wable Raghunandan , Nair Achuth Suresh , Pappu Anirudh , Pierre-Louis Widnie , Abdelhalim Habiba , Patel Khushbu , Mendhe Dinesh , Bolla Shreyas , Mittal Sahil , Ahmed Zeeshan 
Published: January 2023

AbstractTimely understanding of biological secrets of complex diseases will ultimately benefit millions of individuals by reducing the high risks for mortality and improving the quality of life with personalized diagnoses and treatments. Due to the advancements in sequencing technologies and reduced cost, genomics data is developing at an unmatched pace and levels to foster translational research and precision medicine. Over ten million genomics datasets have been produced and publicly shared in the year 2022. Diverse and high-volume genomics and clinical data have the potential to broaden the scope of biological discoveries and insights by extracting, analyzing, and interpreting the hidden information. However, the current and still unresolved challenges include the integration of genomic profiles of the patients with their medical records. The disease definition in genomics medicine is simplified, when in the clinical world, diseases are classified, identified, and adopted with their International Classification of Diseases (ICD) codes, which are maintained by the World Health Organization (WHO). Several biological databases have been produced, which includes information about human genes and related diseases. However, still, there is no database exists, which can precisely link clinical codes with relevant genes and variants to support genomic and clinical data integration for clinical and translation medicine. In this project, we are focused on the development of an annotated gene-disease-code database, which is accessible through an online, cross-platform, and user-friendly application i.e., PAS-GDC. However, our scope is limited to the integration of ICD-9 and ICD-10 codes with the list of genes approved by the American College of Medical Genetics and Genomics (ACMG). Results include over seventeen thousand diseases and four thousand ICD codes, and over eleven thousand gene-disease-code combinations.
Genetically proxied inhibition of L-2-hydroxyglutarate dehydrogenase and the risk of coronary artery disease: A Mendelian randomization study
Authors: Song Euijun 
Published: January 2023

AbstractBackgroundL-2-hydroxyglutarate dehydrogenase (L2HGDH) deletion-induced L-2-hydroxyglutarate accumulation plays a cardioprotective role in hypoxic conditions. However, there has been no causal evidence in real-world clinical data. We aimed to examine the causal effects ofL2HGDHinhibition on coronary artery disease (CAD) and myocardial infarction (MI) using Mendelian randomization (MR) analysis.MethodsWe used nineL2HGDH-proxied genetic variants associated with blood 2-hydroxyglutarate levels as genetic instruments, and performed two-sample MR analysis using the CARDIoGRAMplusC4D meta-analysis datasets of CAD (60,801 CAD cases and 123,504 controls) and MI (34,541 MI cases and 261,984 controls).ResultsGenetically proxied inhibition ofL2HGDHassociated with 2-hydroxyglutarate levels potentially decreased the risk of CAD (odds ratio [OR] 0.486, 95% confidence interval [CI] 0.242–0.977, P=0.043) but was not associated with the risk of MI (OR 0.676, 95% CI 0.312–1.463, P=0.320). This potentially causal association betweenL2HGDHinhibition and CAD was unlikely to be biased by horizontal pleiotropy, whereas there might be a weak instrument bias.ConclusionOur MR analysis suggests the potential association between genetically proxied inhibition ofL2HGDHand CAD. Our findings may have therapeutic implications for L2HGDH inhibitors in CAD, and further large-scale clinical studies are needed.
G3DC: a Gene-Graph-Guided selective Deep Clustering method for single cell RNA-seq data
Authors: He Shuqing , Fan Jicong , Yu Tianwei 
Published: January 2023

AbstractSingle-Cell RNA sequencing (scRNA-seq) technology measures the expression of thousands of genes at the cellular level. Analyzing single cell transcriptome allows the identification of heterogeneous cell groups, cellular-level regulations, and the trajectory of cell development. An important aspect in the analyses of scRNA-seq data is the clustering of cells, which is hampered by issues such as high dimensionality, cell type imbalance, redundancy, and dropout. Given cells of each type are functionally consistent, incorporating biological relations between genes may improve the clustering results. Here, we develop a deep embedded clustering method, G3DC, that incorporates a graph loss based on existing gene network, together with a reconstruction loss to achieve both discriminative and informative embedding. The involvement of the gene network strengthens clustering performance, while helping the selection of functionally coherent genes that contribute to the clustering results. In addition, this method is well adapted to the sparse and zero-inflated scRNA-seq data with theℓ2,1-norm involved. Extensive experiments have shown that G3DC offers high clustering accuracy with regard to agreement with true cell types, outperforming other leading single-cell clustering methods. In addition, G3DC selects biologically relevant genes that contribute to the clustering, providing insight into biological functionality that differentiate cell groups.
Deciphering drug response and phenotypic heterogeneity of cancer cells using gene ensembles of regulatory units defined by chromatin domains
Authors: Pandey Neetesh , Sharma Madhu , Mathur Arpit , Anene-Nzel Chukwuemeka George , Hakimullah Muhammad , Patel Priyanka , Jha Indra Prakash , Chandra Omkar , Mishra Shreya , Bhattacharya Jui , Sharma Ankur , Foo Roger , Sandhu Kuljeet , Mandoli Amit , DasGupta Ramanuj , Kumar Vibhor 
Published: January 2023

AbstractThe effect of co-localization of genes in the topologically associated domains (TADs) and their activity as a regulatory unit in cancer samples and cells, together with drug-response, needs comprehensive analysis. Here, we analyzed the activity of TADs using cancer-cell transcriptomes along with chromatin-interaction and epigenome profiles to understand their relationship with drug-response. Our analysis of 819 cancer cell-line transcriptomes revealed that their response to multiple drugs was more correlated with the activity of individual TADs than genes. Applying our approach to 9014 cancer patients’ data (20 different cancer types) also revealed a higher association between survival and the activity of thousands of individual TADs in comparison to their genes. CRISPR-mediated knock-out of regulatory sites inside a TAD associated with cisplatin-response of oral cancer cells and discovery of primate-specific gain of synteny of genes within a TAD containing EGFR gene and its contribution towards cancer malignancy demonstrate greater utility of TAD-activity based analysis.
EHD1-dependent traffic of IGF-1 receptor to the cell surface is essential for Ewing sarcoma tumorigenesis and metastasis
Authors: Chakraborty Sukanya , Bhat Aaqib M. , Mushtaq Insha , Luan Haitao , Kalluchi Achyuth , Mirza Sameer , Storck Matthew D. , Chaturvedi Nagendra , Lopez- Guerrero Jose Antonio , Llombart-Bosch Antonio , Machado Isidro , Scotlandi Katia , Meza Jane L. , Ghosal Gargi , Coulter Donald W. , Rowley M Jordan , Band Vimla , Mohapatra Bhopal C. , Band Hamid 
Published: January 2023

ABSTRACTOverexpression of EPS15 Homology Domain containing 1 (EHD1) has been linked to tumorigenesis but whether its core function as a regulator of intracellular traffic of cell surface receptors plays a role in oncogenesis remains unknown. We establish that EHD1 is overexpressed in Ewing sarcoma (EWS), with high EHD mRNA expression specifying shorter patient survival. ShRNA and CRISPR-knockout with mouseEhd1rescue established a requirement of EHD1 for tumorigenesis and metastasis. RTK antibody arrays identified the IGF-1R as a target of EHD1 regulation in EWS. Mechanistically, we demonstrate a requirement of EHD1 for endocytic recycling and Golgi to plasma membrane traffic of IGF-1R to maintain its surface expression and downstream signaling. Conversely, EHD1 overexpression-dependent exaggerated oncogenic traits require IGF-1R expression and kinase activity. Our findings define the RTK traffic regulation as a proximal mechanism of EHD1 overexpression-dependent oncogenesis that impinges on IGF-1R in EWS, supporting the potential of IGF-1R and EHD1 co-targeting.
Spike substitution T813S increases Sarbecovirus fusogenicity by enhancing the usage of TMPRSS2
Authors: Ma Yong , Li Pengbin , Hu Yunqi , Qiu Tianyi , Wang Lixiang , Lu Hongjie , Lv Kexin , Xu Mengxin , Zhuang Jiaxin , Liu Xue , He Suhua , He Bing , Liu Shuning , Liu Lin , Wang Yuanyuan , Yue Xinyu , Zhai Yanmei , Luo Wanyu , Mai Haoting , Zhao Wenjing , Chen Jun , Chen Shoudeng , Xiong Xiaoli , Shi Mang , Pan Ji-An , Chen Yao-Qing 
Published: January 2023

AbstractSARS-CoV Spike (S) protein shares considerable homology with SARS-CoV-2 S, especially in the conserved S2 subunit (S2). S protein mediates coronavirus receptor binding and membrane fusion, and the latter activity can greatly influence coronavirus infection. We observed that SARS-CoV S is less effective in inducing membrane fusion compared with SARS-CoV-2 S. We identify that S813T mutation is sufficient in S2 interfering with the cleavage of SARS-CoV-2 S by TMPRSS2, reducing spike fusogenicity and pseudoparticle entry. Conversely, the mutation of T813S in SARS-CoV S increased fusion ability and viral replication. Our data suggested that residue 813 in the S was critical for the proteolytic activation, and the change from threonine to Serine at 813 position might be an evolutionary feature adopted by SARS-2-related viruses. This finding deepened the understanding of Spike fusogenicity and could provide a new perspective for exploringSarbecovirus’ evolution.Author SummaryThe Spike strain of SARS-CoV-2 has accumulated many mutations during its time in circulation, most of which have occurred in the S1 region, and more specifically in the RBD, in an effort to either improve the virus’s affinity for the receptor ACE2 or to enhance its ability to evade the immune system. Mutations in the Spike S2 region have more far-reaching effects than those in the S1 region because it is more conserved across sarbecoviruses. By comparing SARS and SARS2, we found that an important substitution at amino acid position 813 in the S2 region (T813S) disrupts the utilization of TMPRSS2 and can significantly influence viral entry into cells. This discovery deepens our knowledge of S proteins and provides new prospects for tracing the evolution of Sarbecoviruses.
Circulating Endothelial Progenitor Cells Reduce the Risk of Alzheimer’s Disease
Authors: Wang Yixuan , Huang Jinghan , Ang Ting Fang Alvin , Zhu Yibo , Tao Qiushan , Mez Jesse , Alosco Michael , Denis Gerald V. , Belkina Anna , Gurnani Ashita , Ross Mark , Gong Bin , Han Jingyan , Lunetta Kathryn L. , Stein Thor D. , Au Rhoda , Farrer Lindsay A. , Zhang Xiaoling , Qiu Wei Qiao 
Published: January 2023

SummaryCerebrovascular damage coexists with Alzheimer’s disease (AD) pathology and increases AD risk. However, it is unclear whether endothelial progenitor cells reduce AD risk via cerebrovascular repair. By using the Framingham Heart Study (FHS) offspring cohort, which includes data on different progenitor cells, the incidence of AD dementia, peripheral and cerebrovascular pathologies, and genetic data (n = 1,566), we found that elevated numbers of circulating endothelial progenitor cells with CD34+CD133+ co-expressions had a dose-dependent association with decreased AD risk (HR = 0.67, 95% CI: 0.46-0.96, p = 0.03) after adjusting for age, sex, years of education, andAPOEε4. With stratification, this relationship was only significant among those individuals who had vascular pathologies, especially hypertension (HTN) and cerebral microbleeds (CMB), but not among those individuals who had neither peripheral nor central vascular pathologies. We applied a genome-wide association study (GWAS) and found that the number of CD34+CD133+ cells impacted AD risk depending on the homozygous genotypes of two genes:KIRREL3rs580382 CC carriers (HR = 0.31, 95% CI: 0.17-0.57, p<0.001),KIRREL3rs4144611 TT carriers (HR = 0.29, 95% CI: 0.15-0.57, p<0.001), andEXOC6Brs61619102 CC carriers (HR = 0.49, 95% CI: 0.31-0.75, p<0.001) after adjusting for confounders. In contrast, the relationship did not exist in their counterpart genotypes, e.g.KIRREL3TT/CT or GG/GT carriers andEXOC6BGG/GC carriers. Our findings suggest that circulating CD34+CD133+ endothelial progenitor cells can be therapeutic in reducing AD risk in the presence of cerebrovascular pathology, especially inKIRREL3andEXOC6Bgenotype carriers.