bluuebunny/crossref_metadata_2025_split huggingface dataset:
Socioeconomic differentials of trends in the prevalence and economic burden of chronic obstructive pulmonary disease in rural southwest China
Authors: Cai Le , Wang Xu-Ming , Liu Lan , Zhao Yi , Golden Allison Rabkin 
Published: January 2023

Abstract
Background
Chronic obstructive pulmonary disease (COPD) is a leading cause of mortality and morbidity, and imposes a substantial financial burden on society. However, few studies have examined the role of individual socioeconomic status (SES) in temporal trends of COPD prevalence and economic cost. This study aimed to uncover the changing prevalence and economic burden of COPD across socioeconomic gradients in rural southwest China.

Methods
Data were collected from two cross-sectional health interviews and examination surveys administered 10 years apart among individuals aged ≥ 35 years in rural China. A prevalence-based cost-of-illness method was used to estimate the cost of COPD. The individual socioeconomic position (SEP) index was constructed using principal component analysis. Post-bronchodilator spirometry tests were performed for each participant.

Results
From 2011 to 2021, the prevalence of COPD increased from 8.7% to 12.8% (P < 0.01), while the economic cost of COPD increased 1.9-fold. Unit hospital costs and outpatient costs increased 1.57–fold and 1.47–fold, while unit medication costs fell by 10.6%. Increasing prevalence was also observed when the data were stratified by sex, age, ethnicity, level of education, level of income, and SEP (P < 0.05). Men, ethnic minorities, and those with a lower educational level, lower income, or lower SEP had a higher prevalence of COPD than their counterparts both in 2011 and 2021 (P < 0.05). Unit outpatient costs and medication costs increased with patients’ SEP in both survey years (P < 0.05).

Conclusions
The prevalence and economic costs of COPD increased substantially across all socioeconomic gradients in rural southwest China in the decade from 2011 and 2021. Future COPD prevention and management interventions as well as efforts to improve access to affordable COPD medication and treatment should focus in particular on ethnic minority and low SEP populations.
A review of 14 cases of perianal Paget’s disease: characteristics of anorectal cancer with pagetoid spread
Authors: Imaizumi Jun , Moritani Konosuke , Takamizawa Yasuyuki , Inoue Manabu , Tsukamoto Shunsuke , Kanemitsu Yukihide 
Published: January 2023

Abstract
Background
Perianal Paget’s disease (PPD) is an intraepithelial invasion of the perianal skin and is frequently associated with underlying anorectal carcinoma. The relatively rare nature of this disease has made it difficult to develop treatment recommendations. This study aims to analyze the clinical and pathological features of perianal Paget’s disease (PPD) and to explore rational treatment options and follow-up for this disease.

Methods
The National Cancer Center Hospital database was searched for all cases of perianal Paget’s disease diagnosed between 2006 and 2021. In the 14 patients identified, we reviewed the diagnosis, management, and outcomes of adenocarcinoma with pagetoid spread, including suspected or recurrent cases.

Results
All 14 cases met the inclusion criteria. The median follow-up period after diagnosis was 4.5 (range, 0.1–13.0) years. Pagetoid spread before initial treatment was suspected in 12 cases (85.7%). Underlying rectal cancer was identified in 6 cases, and no primary tumor was detected in the other 6 cases. Seven patients had recurrent disease, with the median time to recurrence of 34.6 (range, 19.2–81.7) months. The time to the first relapse was 3 months, and that to the second relapse was 6 months. The overall 5-year survival rate was 90.0%.

Conclusions
Endoscopic and radiologic evaluation, as well as immunohistologic examination, should be performed. is to differentiate PPD with and without underlying anorectal carcinoma. The time to first recurrence varies widely, and long-term and regular follow-up for more than 5 years is considered necessary for local recurrence and distant metastasis.
A new PEDV strain CH/HLJJS/2022 can challenge current detection methods and vaccines
Authors: Yao Xin , Qiao Wen-Ting , Zhang Yu-Qian , Lu Wei-Hong , Wang Zhen-Wei , Li Hui-Xin , Li Jin-Long 
Published: January 2023

AbstractBackgroundPorcine epidemic diarrhea virus (PEDV) variant strains cause great economic losses to the global swine industry. However, vaccines do not provide sufficient protection against currently circulating strains due to viral mutations. This study traced the molecular characteristics of the most recent isolates in China and aimed to provide a basis for the prevention and treatment of PEDV.MethodsWe obtained samples from a Chinese diarrheal swine farm in 2022. Reverse transcription polymerase chain reaction and immunofluorescence were used to determine the etiology, and the full-length PEDV genome was sequenced. Nucleotide similarity was calculated using MEGA to construct a phylogenetic tree and DNASTAR. Mutant amino acids were aligned using DNAMAN and modeled by SWISS-MODEL, Phyre2 and FirstGlance in JMOL for protein tertiary structure simulation. Additionally, TMHMM was used for protein function prediction.ResultsA PEDV virulent strain CH/HLJJS/2022 was successfully isolated in China. A genome-wide based phylogenetic analysis suggests that it belongs to the GII subtype, and 96.1–98.9% homology existed in the whole genomes of other strains. For the first time, simultaneous mutations of four amino acids were found in the highly conserved membrane (M) and nucleocapsid (N) proteins, as well as eight amino acid mutations that differed from the vast majority of strains in the spike (S) protein. Three of the mutations alter the S-protein spatial structure. In addition, typing markers exist during strain evolution, but isolates are using the fusion of specific amino acids from multiple variant strains to add additional features, as also demonstrated by protein alignments and 3D models of numerous subtype strains.ConclusionThe newly isolated prevalent strain CH/HLJJS/2022 belonged to the GII subtype, and thirteen mutations different from other strains were found, including mutations in the highly conserved m and N proteins, and in the S1° and COE neutralizing epitopes of the S protein. PEDV is breaking through original cognitions and moving on a more complex path. Surveillance for PEDV now and in the future and improvements derived from mutant strain vaccines are highly warranted.
Inhibiting the glycerophosphodiesterase EDI3 in ER-HER2+ breast cancer cells resistant to HER2-targeted therapy reduces viability and tumour growth
Authors: Keller Magdalena , Rohlf Katharina , Glotzbach Annika , Leonhardt Gregor , Lüke Simon , Derksen Katharina , Demirci Özlem , Göçener Defne , AlWahsh Mohammad , Lambert Jörg , Lindskog Cecilia , Schmidt Marcus , Brenner Walburgis , Baumann Matthias , Zent Eldar , Zischinsky Mia-Lisa , Hellwig Birte , Madjar Katrin , Rahnenführer Jörg , Overbeck Nina , Reinders Jörg , Cadenas Cristina , Hengstler Jan G. , Edlund Karolina , Marchan Rosemarie 
Published: January 2023

Abstract
Background
Intrinsic or acquired resistance to HER2-targeted therapy is often a problem when small molecule tyrosine kinase inhibitors or antibodies are used to treat patients with HER2 positive breast cancer. Therefore, the identification of new targets and therapies for this patient group is warranted. Activated choline metabolism, characterized by elevated levels of choline-containing compounds, has been previously reported in breast cancer. The glycerophosphodiesterase EDI3 (GPCPD1), which hydrolyses glycerophosphocholine to choline and glycerol-3-phosphate, directly influences choline and phospholipid metabolism, and has been linked to cancer-relevant phenotypes in vitro. While the importance of choline metabolism has been addressed in breast cancer, the role of EDI3 in this cancer type has not been explored.

Methods
EDI3 mRNA and protein expression in human breast cancer tissue were investigated using publicly-available Affymetrix gene expression microarray datasets (n = 540) and with immunohistochemistry on a tissue microarray (n = 265), respectively. A panel of breast cancer cell lines of different molecular subtypes were used to investigate expression and activity of EDI3 in vitro. To determine whether EDI3 expression is regulated by HER2 signalling, the effect of pharmacological inhibition and siRNA silencing of HER2, as well as the influence of inhibiting key components of signalling cascades downstream of HER2 were studied. Finally, the influence of silencing and pharmacologically inhibiting EDI3 on viability was investigated in vitro and on tumour growth in vivo.

Results
In the present study, we show that EDI3 expression is highest in ER-HER2 + human breast tumours, and both expression and activity were also highest in ER-HER2 + breast cancer cell lines. Silencing HER2 using siRNA, as well as inhibiting HER2 signalling with lapatinib decreased EDI3 expression. Pathways downstream of PI3K/Akt/mTOR and GSK3β, and transcription factors, including HIF1α, CREB and STAT3 were identified as relevant in regulating EDI3 expression. Silencing EDI3 preferentially decreased cell viability in the ER-HER2 + cells. Furthermore, silencing or pharmacologically inhibiting EDI3 using dipyridamole in ER-HER2 + cells resistant to HER2-targeted therapy decreased cell viability in vitro and tumour growth in vivo.

Conclusions
Our results indicate that EDI3 may be a potential novel therapeutic target in patients with HER2-targeted therapy-resistant ER-HER2 + breast cancer that should be further explored.
NUF2 promotes tumorigenesis by interacting with HNRNPA2B1 via PI3K/AKT/mTOR pathway in ovarian cancer
Authors: Ren Meng , Zhao Hongyu , Gao Yan , Chen Qi , Zhao Xiaoting , Yue Wentao 
Published: January 2023

Abstract
Background
Ovarian cancer (OC) is one of the commonest and deadliest diseases that threaten the health of women worldwide. It is essential to find out its pathogenic mechanisms and therapeutic targets for OC patients. Although NUF2 (Ndc80 kinetochore complex component) has been suggested to play an important role in the development of many cancers, but little is known about its function and the roles of proteins that regulate NUF2 in OC. This study aimed to investigate the effect of NUF2 on the tumorigenicity of OC and the activities of proteins that interact with NUF2.

Methods
Oncomine database and immunohistochemical (IHC) staining were used to evaluate the expression of NUF2 in OC tissues and normal tissues respectively. Normal ovarian epithelial cell lines (HOSEpiC) and OC cell lines (OVCAR3、HEY、SKOV3) were cultured. Western blot was applied to analyze the expression of NUF2 in these cell lines. Small interfering RNA (siRNA) was used to silence the expression of NUF2 in OC cell lines, SKOV3 and HEY. Gene Set Variation Analysis (GSVA), Gene Set Enrichment Analysis (GSEA), the CCK-8 method, colony formation assay and flow cytometry were conducted to analyze the biological functions of NUF2 in vitro. OC subcutaneous xenograft tumor models were used for in vivo tests. Immunoprecipitation and mass spectrometry (IP/MS) were performed to verify the molecular mechanisms of NUF2 in OC. IP, immunofluorescence, IHC staining, and Gene Expression Profiling Interactive Analysis platform (GEPIA) were used to analyze the relationship between HNRNPA2B1 and NUF2 in OC cells. SiRNA was used to silence the expression of HNRNPA2B1 in SKOV3 cells, reverse transcription quantitative polymerase chain reaction (RT-qPCR) assay and western blot were used to detect the effect of HNRNPA2B1 on NUF2. GEPIA, The Cancer Genome Atlas (TCGA) database, GSEA and western blot were used to detect the potential signaling pathways related to the roles of HNRNPA2B1 and NUF2 in OC cells.

Results
Our results showed high NUF2 expression in OC tissues and OC cell lines, which was associated with shorter overall survival and progression-free survival in patients. NUF2 depletion by siRNA suppressed the proliferation abilities and induced cell apoptosis of OC cells in vitro, and impeded OC growth in vivo. Mechanistically, NUF2 interacted with HNRNPA2B1 and activated the PI3K/AKT/mTOR signaling pathway in OC cells.

Conclusion
NUF2 could serve as a prognostic biomarker, and regulated the carcinogenesis and progression of OC. Moreover, NUF2 may interact with HNRNPA2B1 by activating the PI3K/AKT/mTOR signaling pathway to promote the development of OC cells. Our present study supported the key role of NUF2 in OC and suggested its potential as a novel therapeutic target.
Wavelet transforms based ARIMA-XGBoost hybrid method for layer actions response time prediction of cloud GIS services
Authors: Li Jiang , Cai Jing , Li Rui , Li Qiang , Zheng Lina 
Published: January 2023

AbstractLayer actions response time is a critical indicator of cloud geographical information services (cloud GIS Services), which is of great significance to resource allocation and schedule optimization. However, since cloud GIS services are highly dynamic, uncertain, and uncontrollable, the response time of layer actions is influenced by spatiotemporal intensity and concurrent access intensity, posing significant challenges in predicting layer action response time.To predict the response time of layer actions more accurately, we analyzed the data association of cloud GIS services. Furthermore, based on the characteristics of long-term stable trends and short-term random fluctuations in layer actions response time series, a wavelet transforms-based ARIMA-XGBoost hybrid method for cloud GIS services is proposed to improve the one-step and multi-step prediction results of layer actions response time.We generate a multivariate time series feature matrix using the historical value of the layer actions response time, the predicted value of the linear component, and the historical value of the non-linear component. There is no need to meet the traditional assumption that the linear and nonlinear components of the time series are additive, which minimizes the model’s time series requirements and enhances its flexibility. The experimental results demonstrate the superiority of our approach over previous models in the prediction of layer actions response time of cloud GIS services.
Cloud trains associated with Martian Mountain Lee Waves on the eastern side of the Phlegra Montes
Authors: Ogohara Kazunori , Ro Maaya 
Published: January 2023

AbstractMountain lee waves have often been observed on the eastern side of the Phlegra Montes as wave trains visualized by water ice clouds in the Martian atmosphere. The seasonality and formation condition of these lee waves and associated cloud trains have not yet been investigated, whereas those of Martian dust storms have been studied observationally and numerically. We extract the cloud trains in this region from images observed by the Mars Orbiter Camera onboard the Mars Global Surveyor and measure the wavelengths of the lee waves. It is revealed that, on the eastern side of the Phlegra Montes, cloud trains tend to form in the northern winter season, except during the 2001 global dust storm. The results suggest that stationary mountain waves are excited in the thermally stable atmosphere, but are trapped below an altitude of approximately 10 km due to the zonal wind structure that increases rapidly with altitude. This is consistent with the previous studies on gravity waves in the Martian atmosphere and is the first study to constrain the typical altitude of the cloud trains from imager observations.
Graphical Abstract
Investigating strategies to improve AccesS to Kidney transplantation (the ASK trial): a protocol for a feasibility randomised controlled trial with parallel process evaluation
Authors: Bailey Pippa K. , Caskey Fergus J. , MacNeill Stephanie , Ashford Rachel , Pryce Lindsay , Kayler Liise , Ben-Shlomo Yoav 
Published: January 2023

Abstract

Background
The UK’s living-donor kidney transplant (LDKT) activity falls behind that of many other countries internationally, with less than 20% of those eligible receiving a LDKT each year. Certain individuals with kidney disease in the UK appear to be particularly disadvantaged in accessing a LDKT; the most socioeconomically deprived people with kidney disease are 60% less likely to receive a LDKT than the least deprived. Improving equity in living-donor kidney transplantation has been highlighted as an international research priority.

Methods
This feasibility trial was designed to determine the feasibility of delivery and acceptability of a multicomponent intervention designed to improve access to living-donor kidney transplantation. The intervention comprises three main components: (i) a meeting between a home educator and the transplant candidate for a dedicated discussion about living-donor kidney transplantation, living kidney donation and potential donors; (ii) a standardized letter from a healthcare professional to a candidate’s potential donors and (iii) a home-based education and family engagement session including two home educators, the transplant candidate and their family. The primary objectives are to establish the feasibility (i) of delivering the developed intervention in existing care pathways and (ii) of undertaking a randomised controlled trial of the intervention. A mixed-methods parallel process evaluation will investigate the acceptability, implementation and mechanisms of impact of the intervention. The trial is based at two UK hospitals: a transplanting hospital and a non-transplanting referral hospital. Individuals are eligible if they are ≥ 18 years old, are active on the kidney transplant waiting list or have been referred for transplant listing and do not have a potential living-donor undergoing surgical assessment. Randomisation will be undertaken with concealed allocation. Participants will be randomly allocated 1:1 to (i) the intervention or (ii) usual care, stratified by site to ensure a balance in terms of local differences. Minimisation will be used to ensure balance in sex, age group and socioeconomic strata, with probability weighting of 0.8 in order to reduce predictability. The primary outcomes are recruitment (% of those eligible and invited who consent to randomisation) and retention (% of participants completing follow-up).

Discussion
Findings will inform the design of a future fully powered, randomised controlled trial to formally evaluate the effectiveness of the intervention at improving equitable access to living-donor kidney transplantation.

Trial registration
ISRCTN Registry ISRCTN10989132 Applied 30/10/20.
Building transformative city-university sustainability partnerships: the Audacious Partnerships Process
Authors: Keeler Lauren Withycombe , Beaudoin Fletcher , Cid Abril , Cowley Robert , Fahy Samantha , Lerner Amy , Moran Caroline , Torney Diarmuid 
Published: January 2023

AbstractCity governments and urban universities are well-positioned to play critical roles in advancing urban sustainability transformations. However, in partnering, cities and universities often focus efforts on discrete sustainability-related projects, neglecting the development of long-term relationships and deep, inter-organizational ties that can allow for collaboration on lasting and transformational change. Yet, at both cities and universities there are often individuals who are deeply interested in developing better partnerships that contribute to the sustainability and livability of their communities. This research develops and tests an evidence-based and facilitated process to guide sustainability researchers and municipal practitioners in the development of transformational City-university partnerships for sustainability. The Audacious Partnerships Process was tested by four City-university partnerships including Arizona State University and the City of Tempe, Dublin City University and the City of Dublin, King’s College London and the City of Westminster and the National Autonomous University of Mexico and Mexico City. The Audacious Partnerships Process as well as results from post-surveys and interviews following implementation are elaborated. We conclude with key lessons for modifying and implementing the process to contribute to transformative partnership development.
Self-disclosure, mindfulness, and their relationships with happiness and well-being
Authors: Aldahadha Basim 
Published: January 2023

Abstract
Background
This study aims to test how self-disclosure relates to mindfulness, whether self-disclosure or mindfulness best predicts happiness and well-being, and whether there are significant differences between happiness and well-being levels due to self-disclosure and mindfulness. Data from a public sample was collected using e-mails and social media platforms (N = 486).

Results
The findings demonstrated a weak relationship between mindfulness and self-disclosure (r = 0.16) and acting with awareness but a strong relationship between happiness, wellness, mindfulness, and its factors. The results revealed four values that predict the differentiation between happy and unhappy people, and these values are statistically significant, namely, mindfulness, description, acceptance without judgment, and self-disclosure, as well as four values that predict the distinction between being well and unwell, namely mindfulness, observation, description, and self-disclosure. Additionally, results showed significant differences in the means of each of the mindfulness predictor variables in favor of happiness, wellness, and females. For the self-disclosure variable, the results showed differences in its means favoring females, happiness, and wellness. Finally, when controlling for gender and age, regression analyses found mindfulness to be an important predictor for happiness and well-being at 59% and 48% of the variance, respectively, while self-disclosure was a weak predictor at 12% and 15%, respectively.

Conclusions
In general, the results indicate that mindfulness is more effective and beneficial for living a happy and healthy life than self-disclosure