bluuebunny/crossref_metadata_2025_split huggingface dataset:
Comparison of two Phaeodactylum tricornutum ecotypes under nitrogen starvation and resupply reveals distinct lipid accumulation strategies but a common degradation process
Authors: Murison Victor , Hérault Josiane , Côme Martine , Guinio Sabrina , Lebon Alexis , Chamot Christophe , Bénard Magalie , Galas Ludovic , Schoefs Benoît , Marchand Justine , Bardor Muriel , Ulmann Lionel 
Published: September 2023

IntroductionPhaeodactylum tricornutum is a model species frequently used to study lipid metabolism in diatoms. When exposed to a nutrient limitation or starvation, diatoms are known to accumulate neutral lipids in cytoplasmic lipid droplets (LDs). Those lipids are produced partly de novo and partly from the recycle of plastid membrane lipids. Under a nitrogen resupply, the accumulated lipids are catabolized, a phenomenon about which only a few data are available. Various strains of P. tricornutum have been isolated around the world that may differ in lipid accumulation patterns.MethodsTo get further information on this topic, two genetically distant ecotypes of P. tricornutum (Pt1 and Pt4) have been cultivated under nitrogen deprivation during 11 days followed by a resupply period of 3 days. The importance of cytoplasmic LDs relative to the plastid was assessed by a combination of confocal laser scanning microscopy and cell volume estimation using bright field microscopy pictures.Results and discussionWe observed that in addition to a basal population of small LDs (0.005 μm3 to 0.7 μm3) present in both strains all along the experiment, Pt4 cells immediately produced two large LDs (up to 12 μm3 after 11 days) while Pt1 cells progressively produced a higher number of smaller LDs (up to 7 μm3 after 11 days). In this work we showed that, in addition to intracellular available space, lipid accumulation may be limited by the pre-starvation size of the plastid as a source of membrane lipids to be recycled. After resupplying nitrogen and for both ecotypes, a fragmentation of the largest LDs was observed as well as a possible migration of LDs to the vacuoles that would suggest an autophagic degradation. Altogether, our results deepen the understanding of LDs dynamics and open research avenues for a better knowledge of lipid degradation in diatoms.
The subject-environment interplay between runners from different Brazilian macro-regions
Authors: Thuany Mabliny , Bandeira Paulo Felipe Ribeiro , Vieira Douglas , Weiss Katja , Knechtle Beat , Gomes Thayse Natacha 
Published: September 2023

PurposeOur purpose was to investigate the interplay between runners and their environment using a network approach.MethodsThis cross-sectional study sampled Brazilian runners of both sexes, from the five macro-regions of the country. An electronic questionnaire was used to obtain information regarding age, sex, training volume, socio-economic level, place of residence, and running pace. Environmental indicators (public illumination, pavement, sidewalk, and green areas) were collected from available public information. Descriptive statistics were presented in mean (SD), and frequency (%). A network analysis was performed to evaluate the association between individual and environmental characteristics. Statistical analyses were performed in the JASP, considering p &lt; 0.05.ResultsAt North and Mid-West regions, public illumination presents the highest values for the expected influence (1.74 and 1.56), while in Northeast and Southeast, sidewalks present the highest values (2.13; 0.91). For betweenness centrality, in North, Northeast, and Mid-West regions, residency in the capital of a state presented a hub. In contrast, pavement, and training volume present higher values in the South and Southeast. Network topologies are different.ConclusionPublic illumination (North and Mid-West) and sidewalk (Northeast, Southeast) were the most important variables for runners. Continental size countries need specific approaches to improve physical activity levels and health outcomes that consider the cultural, historical, and environmental background.
The association between working memory, teacher-student relationship, and academic performance in primary school children
Authors: Sankalaite Simona , Huizinga Mariëtte , Warreyn Petra , Dewandeleer Jolien , Baeyens Dieter 
Published: September 2023

IntroductionEarly relationships with teachers play an important role in children’s development and significantly influence students’ cognitive and academic performance. Studies suggest that working memory (WM) is a strong predictor of academic achievement, especially of reading and arithmetic outcomes. The associations between teacher-student relationship (TSR) quality, children’s WM skills and their academic performance have been reported in numerous observational studies. However, the potentially bidirectional and temporal nature of the relationships between these constructs is understudied.MethodsThe purpose of this study was to investigate the relationships between primary school children’s WM and TSR by applying a cross-lagged design and measuring these constructs at three time points throughout the academic year. More exploratively, this study investigated how WM and TSR bidirectionally relate to children’s academic performance.ResultsThe findings of this study revealed a temporal relationship between WM and TSR: between WM-related problems in the classroom at baseline and conflict at 3-month follow-up, and between closeness at 3-month follow-up and WM-related problems in the classroom at 5-month follow-up. Moreover, the findings showed a bidirectional relationship between arithmetic performance and WM-related problematic behaviour.DiscussionThis study highlights that relationships between the teacher and students play an important role in supporting students’ cognitive and academic development. Importantly, this study suggests that children with WM problems may benefit from interventions that focus on improving their relationships with teachers. Additionally, the findings propose that interventions targeting WM may also have positive effects on children’s academic performance.
Effect of coercive measures on treatment outcome in involuntarily admitted patients in Amsterdam
Authors: van der Post L. F. M. , Nusselder K. J. , Peen J. , Nabitz U. , Dekker J. M. 
Published: September 2023

ObjectiveThe prevalence of involuntary admissions rose the last forty years in European countries, including the Netherlands. Involuntary admissions result in seclusion, physical restraint and forced medication in approximately 40% of patients. We looked at whether treatment outcomes differ in patients with and without coercive measures.MethodsUsing The Health of the Nation Outcome Scales (HoNOS) to measure treatment outcomes, we studied the files of 786 patients admitted involuntarily to an Amsterdam clinic. We applied Generalised Linear Models to determine whether the use, or not, of coercive measures during treatment was associated with a difference in outcomes.Results19% of the cohort were secluded in a High Security Room (HSR); 24% were secluded in their own room and/or received forced medication. After adjustment for the influence of diagnosis, disorder severity (initial HoNOS score) and treatment duration, the HSR group had, on average, a HoNOS difference score that was 2.4 points lower than patients without coercive measures (CI −4.0 to −0.8.; p 0.003). In the seclusion in own room group, this score was 2.6 points lower (CI −4.0 to −1.1; p 0.001), corresponding to an effect size of 0.35 and 0.40, respectively.ConclusionSeclusion, whether or not in combination with forced medication, was applied to two-fifths of patients. The HoNOS scores of the group without coercion improved by nearly two and a half points more on average than those of the two groups with coercion. A causal relationship between coercion and treatment outcome could neither be confirmed nor excluded on the basis of our results.
Caring load and family caregivers’ burden in China: the mediating effects of social support and social exclusion
Authors: Hu Hongwei , Hu Xinyi , Xu Yang 
Published: September 2023

ObjectiveCaring for older adults with disabilities is a source of stress for family caregivers, and the lack of social support and the pressure of social exclusion might aggravate family caregiver burden. This study aimed to examine the association between caring load and family caregivers’ burden, as well as the mediating effects of social support and social exclusion.MethodsData used in this study was derived from the nationally representative database of the aged population in China, and 3,125 households with disabled old adults and their home-bound caregivers were eventually selected for this analysis. Regression methods and mediation analysis methods were employed in this study.ResultsThe results indicated that there was a significant positive association between caring load and the caregiver burden, and specifically, social support intensity (rather than social support breadth) and passive social exclusion (rather than active social exclusion) played partial mediating effects. Furthermore, the contributions of mediating effects of social support intensity and passive social exclusion were 13–15 and 27–29%, respectively, and the total contribution of mediating effects was about 35–38%.ConclusionFamily caregivers’ burden should be paid more attention to in the large population with rapidly aging speed like China, and more guidance services as well as support should be provided to family caregivers. In addition, it is crucial to focus on the community’s social support and social exclusion in public policy innovation.
Prolonged grief during and beyond the pandemic: factors associated with levels of grief in a four time-point longitudinal survey of people bereaved in the first year of the COVID-19 pandemic
Authors: Harrop Emily , Medeiros Mirra Renata , Goss Silvia , Longo Mirella , Byrne Anthony , Farnell Damian J. J. , Seddon Kathy , Penny Alison , Machin Linda , Sivell Stephanie , Selman Lucy E. 
Published: September 2023

BackgroundThe COVID-19 pandemic has been a devastating and enduring mass-bereavement event, with uniquely difficult sets of circumstances experienced by people bereaved at this time. However, little is known about the long-term consequences of these experiences, including the prevalence of Prolonged Grief Disorder (PGD) and other conditions in pandemic-bereaved populations.MethodsA longitudinal survey of people bereaved in the UK between 16 March 2020 and 2 January 2021, with data collected at baseline (n = 711), c. 8 (n = 383), 13 (n = 295), and 25 (n = 185) months post-bereavement. Using measures of Prolonged Grief Disorder (PGD) (Traumatic Grief Inventory), grief vulnerability (Adult Attitude to Grief Scale), and social support (Inventory of Social Support), this analysis examines how participant characteristics, characteristics of the deceased and pandemic-related circumstances (e.g., restricted visiting, social isolation, social support) are associated with grief outcomes, with a focus on symptoms of PGD.ResultsAt baseline, 628 (88.6%) of participants were female, with a mean age of 49.5 (SD 12.9). 311 (43.8%) deaths were from confirmed/suspected COVID-19. Sample demographics were relatively stable across time points. 34.6% of participants met the cut-off for indicated PGD at c. 13 months bereaved and 28.6% at final follow-up. Social isolation and loneliness in early bereavement and lack of social support over time strongly contributed to higher levels of prolonged grief symptoms, while feeling well supported by healthcare professionals following the death was associated with reduced levels of prolonged grief symptoms. Characteristics of the deceased most strongly associated with lower levels of prolonged grief symptoms, were a more distant relationship (e.g., death of a grandparent), an expected death and death occurring in a care-home. Participant characteristics associated with higher levels of prolonged grief symptoms included low level of formal education and existence of medical conditions.ConclusionResults suggest higher than expected levels of PGD compared with pre-pandemic times, with important implications for bereavement policy, provision and practice now (e.g., strengthening of social and specialist support) and in preparedness for future pandemics and mass-bereavement events (e.g., guidance on infection control measures and rapid support responses).
The impact of the synergistic effect of SO2 and PM2.5/PM10 on obstructive lung disease in subtropical Taiwan
Authors: Chen Te-Yu , Chen Szu-Chia , Wang Chih-Wen , Tu Hung-Pin , Chen Pei-Shih , Hu Stephen Chu-Sung , Li Chiu-Hui , Wu Da-Wei , Hung Chih-Hsing , Kuo Chao-Hung 
Published: September 2023

BackgroundChronic Obstructive lung diseases (COPD) are complex conditions influenced by various environmental, lifestyle, and genetic factors. Ambient air pollution has been identified as a potential risk factor, causing 4.2 million deaths worldwide in 2016, accounting for 25% of all COPD-related deaths and 26% of all respiratory infection-related deaths. This study aims to evaluate the associations among chronic lung diseases, air pollution, and meteorological factors.MethodsThis cross-sectional study obtained data from the Taiwan Biobank and Taiwan Air Quality Monitoring Database. We defined obstructive lung disease as patients with FEV1/FVC &lt; 70%. Descriptive analysis between spirometry groups was performed using one-way ANOVA and the chi-square or Fisher’s exact test. A generalized additive model (GAM) was used to evaluate the relationship between SO2 and PM2.5/PM10 through equations and splines fitting.ResultsA total of 2,635 participants were enrolled. Regarding environmental factors, higher temperature, higher relative humidity, and lower rainfall were risk factors for obstructive lung disease. SO2 was positively correlated with PM10 and PM2.5, with correlation coefficients of 0.53 (p &lt; 0.0001) and 0.52 (p &lt; 0.0001), respectively. Additionally, SO2 modified the relative risk of obstructive impairment for both PM10 [β coefficient (β) = 0.01, p = 0.0052] and PM2.5 (β = 0.01, p = 0.0155). Further analysis per standard deviation (per SD) increase revealed that SO2 also modified the relationship for both PM10 (β = 0.11, p = 0.0052) and PM2.5 (β = 0.09, p = 0.0155). Our GAM analysis showed a quadratic pattern for SO2 (per SD) and PM10 (per SD) in model 1, and a quadratic pattern for SO2 (per SD) in model 2. Moreover, our findings confirmed synergistic effects among temperature, SO2 and PM2.5/PM10, as demonstrated by the significant associations of bivariate (SO2 vs. PM10, SO2 vs. PM2.5) thin-plate smoothing splines in models 1 and 2 with obstructive impairment (p &lt; 0.0001).ConclusionOur study showed high temperature, humidity, and low rainfall increased the risk of obstructive lung disease. Synergistic effects were observed among temperature, SO2, and PM2.5/PM10. The impact of air pollutants on obstructive lung disease should consider these interactions.
Nomophobia in university students during COVID-19 outbreak: a cross-sectional study
Authors: Navas-Echazarreta Noelia , Juárez-Vela Raúl , Subirón-Valera Ana Belén , Rodríguez-Roca Beatriz , Antón-Solanas Isabel , Fernández-Rodrigo María Teresa , de Viñaspre-Hernandez Regina Ruiz , Sabater Antonio Martínez , Echániz-Serrano Emmanuel , Saus-Ortega Carles , Santolalla-Arnedo Iván , Satústegui-Dordá Pedro José 
Published: September 2023

IntroductionCurrently, access to the Internet through smartphones has led to their functions going beyond purely communicative ones, allowing the management of massive, instantaneous, and easily accessible information. This research analyzed the differences in smartphone use and the prevalence of nomophobia, mainly according to gender and university degree of Health Sciences students at the University of Zaragoza during the COVID-19 confinement in Spain.MethodsA descriptive cross-sectional study was carried out on a sample of 318 first and second-grade students, who completed an online questionnaire sent to their institutional email, which included sociodemographic questions, other questions about smartphone use, and the Nomophobia Questionnaire (NMP-Q) scale.ResultsCompared to men (n = 58), women (n = 260) were more likely to use their smartphones more intensively daily, as were occupational therapy students compared to the other degree programs studied. The prevalence of nomophobia was moderate, being around the risk of suffering from it. No significant differences in scores for nomophobia among students were found according to gender, university degree, or population nucleus for the nomophobia scores of the students.DiscussionThe present study extends the existing literature on nomophobia by providing results of interest in terms of gender and the exceptional healthcare context of COVID-19. The results suggest that despite intense daily smartphone use young people did not reach severe nomophobia figures. This fact underlines the need for appropriate and healthy technology education. Understanding the characteristics of the populations that use the smartphone the most may help to analyze nomophobia rates and the massive use of the device.
A history of asthma may be associated with grandparents’ exposures to stress and cigarette smoking
Authors: Golding Jean , Tunstall Holly , Gregory Steve , Granell Raquel , Dodd James W. , Iles-Caven Yasmin , Watkins Sarah , Suderman Matthew 
Published: September 2023

Introduction: Within human epidemiological studies, associations have been demonstrated between grandparental exposures during childhood and grandchildren’s outcomes. A few studies have assessed whether asthma has ancestral associations with exposure to cigarette smoking, but results have been mixed so far.Material and methods: In this study we used four generations: (F0 great-grandparents, F1 grandparents, F2 parents, F3 study children) of the Avon Longitudinal Study of Parents and Children (ALSPAC) to determine whether there is evidence of associations between asthma in generations F2 or F3 and exposures to severe trauma in childhood and/or active cigarette smoking during the adolescence of grandmothers and grandfathers in generations F0 and F1 respectively, or of a history of a F0 or F1 grandmother smoking during pregnancy.Results: We have shown that: a) stress exemplified by the death of a F1 grandparent’s parent during the grandparents’ childhood was associated with increased risk of asthma in generation F3, especially if the grandparent involved was the paternal grandmother; b) if the grandparents of generations F0 or F1 smoked during adolescence (i.e. &lt; 17 years), their grandchildren in generations F2 and F3 were more likely to have a history of asthma; c) paternal F1 grandmother’s smoking in pregnancy was associated with her F3 grandchild’s asthma at age 7; d) There were differences between the results for the grandsons and granddaughters of the paternal grandmother with exposure to smoking in adolescence and with smoking in pregnancy. e) The addition of all of the individual exposure variables to the different analyses often provided a considerable increase in goodness of fit compared with only adding demographic factors associated with asthma at P &lt; 0.10 such as social class; this was particularly true when all four exposure variables were combined in one model, suggesting possible synergistic effects between them.Discussion: We have shown associations between all four types of exposure to the grandparents to be associated with asthma in the grandchildren, such that the results both depended on whether the male or female line was involved, and the sex of the grandchildren. It was notable that the paternal grandmother was particularly involved in many of the associations. We emphasize that these are exploratory analyses, that asthma diagnostic criteria likely changed over time and may not be consistent between generations, and that the results should be tested in other cohorts.
A unique case of AH-dominant type nodular pulmonary amyloidosis presenting as a spontaneous pneumothorax: a case report and review of the literature
Authors: Skopelidou Valeria , Hurník Pavel , Tulinský Lubomír , Židlík Vladimir , Lenz Jiří , Delongová Patricie , Hornychová Helena , Flodr Patrik , Jelínek Tomáš , Muroňová Ludmila , Holub Dušan , Džubák Petr , Hajdúch Marián 
Published: September 2023

Amyloidosis is a rare metabolic disorder primarily brought on by misfolding of an autologous protein, which causes its local or systemic deposition in an aberrant fibrillar form. It is quite rare for pulmonary tissue to be impacted by amyloidosis; of the three forms it can take when involving pulmonary tissue, nodular pulmonary amyloidosis is the most uncommon. Nodular pulmonary amyloidosis rarely induces clinical symptoms, and most often, it is discovered accidentally during an autopsy or via imaging techniques. Only one case of nodular pulmonary amyloidosis, which manifested as a spontaneous pneumothorax, was found in the literature. In terms of more precise subtyping, nodular amyloidosis is typically AL or mixed AL/AH type. No publications on AH-dominant type of nodular amyloidosis were found in the literature. We present a case of an 81 years-old male with nodular pulmonary AH-dominant type amyloidosis who presented with spontaneous pneumothorax. For a deeper understanding of the subject, this study also provides a review of the literature on cases with nodular pulmonary amyloidosis in relation to precise amyloid fibril subtyping. Since it is often a difficult process, accurate amyloid type identification is rarely accomplished. However, this information is very helpful for identifying the underlying disease process (if any) and outlining the subsequent diagnostic and treatment steps. Even so, it is crucial to be aware of this unit and make sure it is taken into consideration when making a differential diagnosis of pulmonary lesions.
Biofertilizante “biol”: caracterización física, química y microbiológica
Authors: Gil Ramírez Luis Arturo , Leiva Cabrera Frans Allinson , Lezama Escobedo Martha Karina , Bardales Vásquez Cecilia Betzabet , León Torres Carlos Alberto 
Published: September 2023

El biol es un abono orgánico de consistencia líquida que se produce en un biodigestor a partir de la fermentación del estiércol de animales y de residuos vegetales, este producto se emplea actualmente como un abono foliar, el cual promueve el desarrollo y crecimiento saludable de diversos cultivos. El objetivo de la presente investigación es evaluar las características físico-químicas y microbiológicas del biol de la Estación Experimental de Bioquímica Aplicada de la Facultad de Ciencias Biológicas de la Universidad Nacional de Trujillo (E.E.B. A–CC.BB-UNT). Para la obtención de los datos se empleó un conductímetro, pH-metro, el método de espectroscopia de absorción atómica, el método de fósforo reactivo, método de Kjeldahl, método azul de metileno y el método del Número más Probable y se obtuvo los siguientes datos: 16,32 mS cm-1 para la conductividad eléctrica, 6.90 para el pH, 290,0 mgL-1, 17,78 mgL-1, 111,7 mgL-1, 153,2 mgL-1, 0,360 mgL-1, 0,080 mgL-1, 1,530 mgL-1, 0,300 mgL-1, 0,140 mgL-1 y 0,017 mgL-1, para el nitrógeno, fosforo, potasio, magnesio, sulfuros, cobre, fierro, manganeso, zinc, y cadmio respectivamente, para plomo y cromo se obtuvo valores inferiores a 0,005 mgL-1 ; así también se reportó ausencia de coliformes totales y fecales (< 1,8 NMP/100 mL). El biol del presente estudio cuenta con las características físico-químicas y microbiológicas para un óptimo crecimiento de diversos cultivos, así también, de ser empleado como una alternativa de solución frente a los agroquímicos convencionales que impactan de una manera negativa el sector agrícola y el medio ambiente.