bluuebunny/crossref_metadata_2025_split huggingface dataset:
Resveratrol alleviates lipopolysaccharide-induced liver injury by inducing SIRT1/P62-mediated mitophagy in gibel carp (Carassius gibelio)
Authors: Wu Liyun , Chen Qiaozhen , Dong Bo , Geng Hancheng , Wang Yu , Han Dong , Zhu Xiaoming , Liu Haokun , Zhang Zhimin , Yang Yunxia , Xie Shouqi , Jin Junyan 
Published: April 2023

IntroductionResveratrol (RES) is a polyphenol organic compound with antioxidant and anti-inflammatory properties. This study aimed to determine whether and how RES can alleviate liver injury in lipopolysaccharide (LPS)-induced gibel carp.MethodsGibel carp were fed a diet with or without RES and were cultured for 8 weeks, followed by LPS injection.Results and discussionThe results suggested that RES attenuated the resulting oxidative stress and inflammation by activating the Nrf2/Keap1 pathway and inhibiting the NF-κB pathway, as confirmed by changes in oxidative stress, inflammation-related gene expression, and antioxidant enzyme activity. Furthermore, RES cleared damaged mitochondria and enhanced mitochondrial biogenesis to mitigate reactive oxygen species (ROS) accumulation by upregulating the SIRT1/PGC-1α and PINK1/Parkin pathways and reducing p62 expression. Overall, RES alleviated LPS-induced oxidative stress and inflammation in gibel carp through mitochondria-related mechanisms.
Evaluation of second-line apatinib plus irinotecan as a treatment for advanced gastric adenocarcinoma or gastroesophageal conjunction adenocarcinoma: a prospective, multicenter phase II trial
Authors: Qu Jinglei , He Xin , Luo Ying , Yu Ping , Chen Ying , Liu Jing , Wang Xin , Wang Chang , Liang Tingting , Bai Yuxian , Han Yu , Man Li , Leng Chuanchun , Zhou Caiyun , He Lijie , Wang Xin , Liu Yunpeng , Qu Xiujuan 
Published: April 2023

ObjectiveApatinib and irinotecan are used as systematic therapies for advanced gastric adenocarcinoma (GAC) and gastroesophageal junction adenocarcinoma (GEJA), while the evidence for their combination as second-line therapy in these patients is limited. This study aimed to evaluate the efficacy and safety of second-line apatinib plus irinotecan for the treatment of GAC and GEJA.MethodsIn this prospective, multicenter phase II clinical study, 28 patients with advanced GAC or GEJA who received second-line apatinib plus irinotecan were recruited.ResultsIn total, 1 (3.6%) patient achieved complete response, 7 (25.0%) patients achieved partial response, 13 (46.4%) patients had stable disease, and 4 (14.3%) patients showed progressive disease, while clinical response was not evaluable or not assessed in 3 (10.7%) patients. The objective response rate and disease control rate were 28.6% and 75.0%, respectively. Meanwhile, the median (95% confidence interval (CI)) progression-free survival (PFS) was 4.5 (3.9-5.1) months, and the median (95% CI) overall survival (OS) was 11.3 (7.4-15.1) months. By multivariate Cox regression analysis, male sex, liver metastasis, and peritoneal metastasis were independently associated with worse PFS or OS, while treatment duration ≥5 months was independently associated with better OS. In terms of the safety profile, 89.3% of patients experienced treatment-emergent adverse events of any grade, among which 82.1% of patients had grade 1-2 adverse events and 64.3% of patients had grade 3-4 adverse events.ConclusionApatinib plus irinotecan as second-line therapy achieves a good treatment response and satisfactory survival with tolerable safety in patients with advanced GAC or GEJA.
Causes of death among early-onset colorectal cancer population in the United States: a large population-based study
Authors: Chen Yuerong , He Lanping , Lu Xiu , Tang Yuqun , Luo Guanshui , Chen Yuji , Wu Chaosheng , Liang Qihua , Xu Xiuhong 
Published: April 2023

BackgroundEarly-onset colorectal cancer (EOCRC) has an alarmingly increasing trend and arouses increasing attention. Causes of death in EOCRC population remain unclear.MethodsData of EOCRC patients (1975–2018) were extracted from the Surveillance, Epidemiology, and End Results database. Distribution of death was calculated, and death risk of each cause was compared with the general population by calculating standard mortality ratios (SMRs) at different follow-up time. Univariate and multivariate Cox regression models were utilized to identify independent prognostic factors for overall survival (OS).ResultsThe study included 36,013 patients, among whom 9,998 (27.7%) patients died of colorectal cancer (CRC) and 6,305 (17.5%) patients died of non-CRC causes. CRC death accounted for a high proportion of 74.8%–90.7% death cases within 10 years, while non-CRC death (especially cardiocerebrovascular disease death) was the major cause of death after 10 years. Non-cancer death had the highest SMR in EOCRC population within the first year after cancer diagnosis. Kidney disease [SMR = 2.10; 95% confidence interval (CI), 1.65–2.64] and infection (SMR = 1.92; 95% CI, 1.48–2.46) were two high-risk causes of death. Age at diagnosis, race, sex, year of diagnosis, grade, SEER stage, and surgery were independent prognostic factors for OS.ConclusionMost of EOCRC patients died of CRC within 10-year follow-up, while most of patients died of non-CRC causes after 10 years. Within the first year after cancer diagnosis, patients had high non-CRC death risk compared to the general population. Our findings help to guide risk monitoring and management for US EOCRC patients.
Refinement of the assignment to the ACMG/AMP BS3 and PS3 criteria of eight BRCA1 variants of uncertain significance by integrating available functional data with protein interaction assays
Authors: Caleca Laura , Radice Paolo 
Published: April 2023

The clinical screening of cancer predisposition genes has led to the identification of a large number of variants of uncertain significance (VUS). Multifactorial likelihood models that predict the odds ratio for VUS in favor or against cancer causality, have been developed, but their use is limited by the amount of necessary data, which are difficult to obtain for rare variants. The guidelines for variant interpretation of the American College of Medical Genetics and Genomics along with the Association for Molecular Pathology (ACMG/AMP) state that “well-established” functional studies provide strong support of a pathogenic or benign impact (criteria PS3 and BS3, respectively) and can be used as evidence type to reach a final classification. Moreover, the Clinical Genome Resource Sequence Variant Interpretation Working Group developed rule specifications to refine the PS3/BS3 criteria. Recently, Lira PC et al. developed the “Hi Set” approach that generated PS3/BS3 codes for over two-thousands BRCA1 VUS. While highly successful, this approach did not discriminate a group of variants with conflicting evidences. Here, we aimed to implement the outcomes of the “Hi-set” approach applying Green Fluorescent Protein (GFP)-reassembly assays, assessing the effect of variants in the RING and BRCT domains of BRCA1 on the binding of these domains with the UbcH5a or ABRAXAS proteins, respectively. The analyses of 26 clinically classified variants, including 13 tested in our previous study, showed 100% sensitivity and specificity in identifying pathogenic and benign variants for both the RING/UbcH5a and the BRCTs/ABRAXAS interactions. We derived the strength of evidences generated by the GFP-reassembly assays corresponding to moderate for both PS3 and BS3 criteria assessment. The GFP-reassembly assays were applied to the functional characterization of 8 discordant variants from the study by Lyra et al. The outcomes of these analyses, combined with those reported in the “Hi Set” study, allowed the assignment of ACMG/AMP criteria in favor or against pathogenicity for all 8 examined variants. The above findings were validated with a semi-quantitative Mammalian Two-Hybrid approach, and totally concordant results were observed. Our data contributes in shedding light on the functional significance of BRCA1 VUS and on their clinical interpretation within the ACMG/AMP framework.
Analysis of the operational status of the three-level referral system for urologic ultrasound screening and risk factors for renal pelvic dilatation in high-risk children
Authors: Ji Baowei , Gong Yinv , Zhang Ying , Li Yun , Zhai Yihui , Sun Yinghua , Wang Xiang , Jia Lishan , Xu Hong , Shen Qian 
Published: April 2023

BackgroundCongenital Anomalies of the Kidney and Urinary Tract (CAKUT) are the primary cause of end-stage renal disease in children, early diagnosis and treatment can significantly improve the kidney function. Among CAKUT, renal pelvis dilatation (RPD) due to various causes has the highest detection rate, which can be detected early by postnatal ultrasound screening. Since 2010, the Children's Hospital of Fudan University (CHFU), together with the Minhang District Maternal and Child Health Hospital (MCH) and Community Health Centres (CHCs) of Minhang District has created a three-level referral system for urological ultrasound screening. This study aims to describe the operation of a three-level referral system for ultrasound screening of CAKUT and to select risk factors of RPD in high-risk children.MethodsThe operation of the three-level referral system was assessed by analyzing the screening volume, screening rate, referral rate, and follow-up rate; risk factors of RPD in high-risk children were selected by chi-square test and multivariate logistic regression.ResultsA total of 16,468 high-risk children were screened in ten years, and the screening volume was maintained at about 1,500 cases per year; the screening rate showed a linear increase, from 36.8% in 2010 to 98.2% in 2019; the referral rate from the CHCs to the MCH was 89.9% significantly higher after 2015 than that of 84.7% from 2010 to 2015; the follow-up rate after 2015 was 71.0% significantly higher than that of 46.3% from 2010 to 2015. Multivariate logistic regression analysis showed that the risk of RPD was 1.966 times higher in males than in females, and the risk of moderate to severe RPD was 2.570 times higher in males than in females; the risk of RPD in preterm children was 1.228 times higher than that of full-term children; and the risk of RPD was 1.218 times higher in twins than in singles.ConclusionsThe screening volume of the three-level referral system has remained stable over a decade, with significantly higher screening, referral, and follow-up rates. Males, preterm, and twins are risk factors of RPD in high-risk children; males are also risk factors for moderate to severe RPD in high-risk children.
Dietary N-carbamylglutamate supplementation improves ammonia tolerance of juvenile yellow catfish Pelteobagrus fulvidraco
Authors: Feng Dexiang , Yang Zhiguo , Li Ming 
Published: April 2023

Introduction: Ammonia has been of concern for its high toxicity to animals. N-carbamylglutamate (NCG) can reduce blood ammonia levels in mammals, but studies on ammonia tolerance in fish are insufficient.Methods: Juvenile yellow catfish were fed two levels of NCG (0.00% and 0.05%) for 84 days under three ammonia levels (0.00, 0.08, and 0.16 mg/L NH3).Results and Discussion: The results showed that survival rate (SUR), final body weight (FBW), weight gain (WG), and serum total protein (TP), triglycerides (TG), glucose (Glu), ornithine (Orn), citrulline (Cit) contents, and liver superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), arginase (ARG), ornithine transcarbamylase (OTC) activities decreased with the increase of ammonia levels, on the contrary, feed conversion ratio (FCR), hepatosomatic index (HSI), and serum ammonia, urea, alanine aminotransferase (ALT), aspartate aminotransferase (AST), glutamine (Gln), arginine (Arg) contents, and liver malondialdehyde (MDA), tumor necrosis factor (TNF), interleukin (IL) 1, IL 8 contents, and mRNA expressions of cu/zn sod, cat, gpx, gr, tnf ɑ, il 1, and il 8 were significantly increased. Dietary 0.05% NCG supplementation had higher SUR, FBW, WG, feed intake (FI), whole-body protein, and serum TP, total cholesterol (TC), Glu, citrulline (Cit) contents, and liver SOD, GPx, argininosuccinate synthetase (ASS), argininosuccinate lyase (ASL), inducible nitric oxide synthase (iNOS) activities compared to 0.00% NCG group, but had lower serum ammonia, urea, ALT, AST, Gln, Arg contents, and liver MDA, TNF, IL 1, IL 8 contents, and neuronal nitric oxide synthase activity. At the end of bacterial challenge, cumulative mortality (CM) increased with ammonia levels increased, but serum antibody titer (AT), lysozyme (LYZ) activity, 50% hemolytic complement, immunoglobulin (Ig) contents, respiratory burst (RB), phagocytic indices decreased with ammonia levels increased. CM in 0.05% NCG group was lower than that in 0.00% NCG group, but serum AT, LYZ activity, Ig content, RB in 0.05% NCG group were significantly higher. The correlation analysis found that iNOS was positively correlated with ASS activity. This study indicates that dietary NCG supplementation can improve the ammonia tolerance of yellow catfish, and ASS may also be the target of NCG to activate the urea cycle.
Stigma and posttraumatic growth among COVID-19 survivors during the first wave of the COVID-19 pandemic in Malaysia: a multicenter cross-sectional study
Authors: Azman Nazirah , Nik Jaafar Nik Ruzyanei , Leong Bin Abdullah Mohammad Farris Iman , Abdul Taib Nur Iwana , Mohamad Kamal Nurul Ain , Abdullah Muhammad Najib , Dollah Siti Nordiana , Mohamed Said Mohd Shahrir 
Published: April 2023

BackgroundContracting COVID-19 can cause negative and distressing psychological sequelae, but traumatic stressors may also facilitate the development of positive psychological change beyond an individual’s previous level of adaptation, known as posttraumatic growth (PTG). As a result, studies have investigated the negative effects of COVID-19 on mental health, but data on PTG among patients who have recovered from COVID-19 remains limited. This study aims to evaluate the level of PTG and its associations with stigma, psychological complications, and sociodemographic factors among COVID-19 patients 6 months post-hospitalization.MethodA cross-sectional online survey of 152 COVID-19 patients was conducted after 6 months of being discharged from Hospital Canselor Tuanku Muhriz, MAEPS Quarantine Center, or Hospital Sungai Buloh, Malaysia. Patients completed a set of questionnaires on sociodemographic and clinical data. The Posttraumatic Growth Inventory (PTGI-SF) was used to assess the level of PTG, the Kessler Psychological Distress (K6) was used to measure the degree of psychological distress, the General Anxiety Disorder-7 (GAD-7) was used to evaluate the severity of anxiety symptoms, the Patient Health Questionnaire (PHQ-9) was used to assess the severity of depression symptoms, and the Explanatory Model Interview Catalog Stigma Scale (EMIC-SS) was used to record the degree of perceived stigma toward COVID-19.ResultsThe median PTGI SF score of the respondents was 40.0 (Interquartile range 16.0). Multivariable general linear model with bootstrapping (2,000 replications) revealed factors that significantly predicted PTG, which were at the higher level of the perceived stigma score, at 37 (B = 0.367, 95% CI = 0.041 to 0.691, p = 0.026), among the Malay ethnicity (B = 12.767, 95% CI 38 = 7.541 to 17.993, p &lt; 0.001), retirees (B = −12.060, 95% CI = −21.310 to −2.811, p = 0.011), and those with a history of medical illness (B = 4.971, 95% CI = 0.096 to 9.845, p = 0.046).ConclusionExperiencing stigma contributed to patients’ PTG in addition to psychosocial factors such as ethnicity, history of medical illness, and retirement.
The role of sociodemographic and clinical factors in the initiation and discontinuation of attention deficit hyperactivity disorder medication among young adults in Sweden
Authors: Gémes Katalin , Taipale Heidi , Björkenstam Emma , Rahman Syed , Gustafsson Klas , Tanskanen Antti , Ekselius Lisa , Mittendorfer-Rutz Ellenor , Helgesson Magnus 
Published: April 2023

IntroductionLong-term medication use is a recommended treatment for attention-deficit/hyperactivity disorder (ADHD), however, discontinuation is common. Non-medical factors which might influence initiation and discontinuation are understudied. Therefore, we investigated how different sociodemographic factors and comorbidities were associated with the initiation and discontinuation of ADHD medication use among young adults.Methods and resultsWe conducted a population-based prospective cohort study using individually linked administrative register data, in which we included all individuals residing in Sweden, between the age of 19 and 29 who were first diagnosed with ADHD between January 2006 and December 2016 (n = 59224). ADHD medication initiation was defined as the first prescription of ADHD medication in the period from 3 months before to 6 months after the cohort entry date. Those who initiated ADHD medication were followed up for medication use until discontinuation, death/emigration, or until 2019. Logistic and Cox regression models were used to investigate the associations between sociodemographics, health-related predictors and initiation, as well as discontinuation. Overall, 48.7% of the 41399 individuals initiated ADHD medication, most often methylphenidate (87%). Among the initiators, 15462 (77%) discontinued medication use during the follow-up (median time: 150 days). After mutually adjusting all other predictors, initiation was positively associated with older age, male sex, higher level of education, and negatively associated with living at home with parents, immigrant status, being unemployed during the year before inclusion, being on disability pension, having autism, substance use, schizophrenia-spectrum disorders, other mental disability/developmental disorders, cardiovascular diseases or previous accidents. Discontinuation was positively associated with being born abroad, living in big cities, being unemployed during the year before inclusion, having cancer, and negatively associated with a higher educational level, having depression, anxiety or stress-related disorder, autism spectrum disorder or diabetes.ConclusionBesides medical factors, sociodemographics, such as educational attainment and immigrant status might also play a role in the initiation and discontinuation of ADHD medication use among young adults newly diagnosed with ADHD.
Influence of the Martian crustal magnetic fields on the Mars-solar wind interaction and plasma transport
Authors: Li Guokan , Lu Haoyu , Li Yun , Song Yihui , Cao Jinbin , Li Shibang 
Published: April 2023

The plasma transport process is important for the ionosphere of Mars, which controls the structure of the ionosphere above an altitude of 200 km. Plasma transport from the dayside ionosphere is crucial for producing the nightside ionosphere on Mars. The alteration in dayside plasma transport in the presence of crustal fields may influence the distribution of Martian ionospheric plasma and plasma escape in the magnetotail. This study employed a three-dimensional multispecies magnetohydrodynamic (MHD) model to simulate Mars-solar wind interactions. We show the magnetic field distribution and plasma velocity variation on the Martian day-side. The results indicate that the ion transport from low- to high-solar-zenith-angle areas in the south is inhibited by crustal fields, leading to a reduction in the ion number density and a thinner ionosphere near the southern terminator. Many heavy ions remain in the southern dayside ionosphere rather than moving to the nightside. In addition, the maximum reduction in the tailward flux of the planetary ions calculated by the MHD simulation is more than 50% at the southern terminator, indicating an inhibitory effect of the crustal fields on day-to-night transport. These effects may lead to a reduction in ion number density in the southern nightside ionosphere. Finally, we demonstrate a decrease in the global heavy-ion loss rate.
Las políticas universitarias y de ciencia y tecnología prepandemia, las respuestas frente a la emergencia sanitaria y los desafíos de la “nueva normalidad” en Argentina
Authors: Suasnábar Claudio , Versino Mariana 
Published: April 2023

Este artículo constituye un ensayo de interpretación que, por un lado, analiza en paralelo las políticas universitarias y de ciencia y tecnología en la Argentina de las últimas dos décadas hasta la irrupción de la pandemia de la covid-19. Y por otro, describe y caracteriza de manera tentativa las distintas respuestas y acciones desarrolladas por las universidades nacionales y los organismos estatales de ciencia y tecnología frente a la emergencia sanitaria.
En esta dirección el trabajo recorta dos grandes dimensiones de análisis, por un lado, las tendencias de cambio estructurales en cada uno de los sectores (expansión, estructuras, financiamiento) y, por otro lado, en las orientaciones de política (discursos, instrumentos, gobierno).
Kualitas Pelayanan Room Boy Terhadap Kepuasan Pelanggan di Hotel Six Senses Uluwatu Bali
Authors: Darsiah Anis , Ingkadijaya Rahmat , Osman Ismeth Emier 
Published: April 2023

Penelitian ini bertujuan untuk mengetahui kualitas pelayanan room boy terhadap kepuasan pelanggan di Hotel Six Senses Uluwatu Bali. Jenis penelitian ini menggunakan deskriptif kuantitatif dengan metode analisis regresi berganda. Teknik pengumpulan data dengan menyebarkan kuisioner dengan google form. Teknik pengambilan sampel dengan menggunakan accidental sampling yaitu responden didapatkan dari kebetulan bertemu dengan peneliti di Hotel Six Senses. Populasi dalam penelitian ini adalah seluruh pengunjung yang datang ke Hotel Six Senses. Sedangkan populasi dalam penelitian ini berjumlah 100 responden. Hasil penelitian menunjukkan bahwa tangible, reliability, responsiveness, assurance, dan empathy. pihak hotel lebih meningkatkan kualitas pada aspek-aspek responsiveness (daya tanggap) kepada konsumen yaitu karyawan hotel hendaknya meningkatkan inisiatif dalam membantu konsumen, profesional dalam menjalankan tugasnya serta menciptakan suasana yang damai dan tentram agar tamu merasa nyaman. Keywords : Tangible, Reliability, Responsiveness, Assurance, Empathy, Kepuasan PelangganAbstractThis study aims to determine the quality of room boy service on customer satisfaction at the Six Senses Hotel Uluwatu Bali. This type of research uses descriptive quantitative with multiple regression analysis method. Data collection techniques by distributing questionnaires with google form. The sampling technique used was accidental sampling, where respondents were obtained by chance meeting with researchers at the Six Senses Hotel. The population in this study were all visitors who came to the Six Senses Hotel. While the population in this study amounted to 100 respondents. The results showed that tangible, reliability, responsiveness, assurance, and empathy. this hotel further improves the quality in terms of responsiveness to consumers, namely hotel employees must increase the initiative in helping consumers, professionals in running their duties and create a peaceful and serene atmosphere so that guests feel comfortable. Keywords : Tangible, Reliability, Responsiveness, Assurance, Empathy, Customer Satisfaction