bluuebunny/crossref_metadata_2025_split huggingface dataset:
Associations between Physical Activity, Screen Time, and Mental Health in Youth Hockey Parents
Authors: McFadden Taylor , Bean Corliss , Fortier Michelle 
Published: August 2023

Abstract
Early sport specialization is becoming increasingly popular among youth. While the negative consequences of early specialization on the physical and psychological health of youth athletes have been well documented in the literature, less is known about the influence of youth sport participation on their parents. The purpose of this study was to examine the associations between physical activity, screen time and mental health in youth hockey parents, while considering the role of their child’s level of specialization. Youth hockey parents (N= 91; parents of early specializers n = 81; parents of late specializers n = 10), responded to an online survey. Results of independent samples t-tests indicated that parents of early specializers had poorer emotional well-being (p <.001) and engaged in more screen time (p = .006) compared to parents of late specializers. There were no significant differences in physical activity levels between groups; however, results showed that all parents were insufficiently active. This is concerning as hierarchal multiple regression revealed that physical activity positively predicted mental health (b = .26, p = .02), and specialization played a role (b = .24, p = .03). Findings suggest that early specialization has negative consequences on the health behaviours and mental health of youth hockey parents.
SPOP Expression Is Associated with Immune Function in Pan-Cancer and Cervical Cancer Cell Proliferation
Authors: Xu Yong-Feng , Yang Xiao-Juan , Zhu Qing 
Published: August 2023

Abstract
BACKGROUND: Speckle Type POZ Protein (SPOP), despite its tumor type-dependent role in tumorigenesis, primarily as a tumor suppressor gene is associated with a variety of different cancers. However, the clinicopathological and immune functional mechanism significance of SPOP among pan-cancer have not been studied. In addition, its function in cervical cancer remains unclear.
METHODS: Here, we aimed to investigate the function of SPOP in 23 types of tumors by using The Cancer Genome Atlas (TCGA), Genotype-Tissue Expression (GTEx), The Tumor Immune Estimation Resource 2.0 (TIMER2.0) database, cBioportal, and various bioinformatic databases. CCK8 assay and colony-formation were used to analysis the effect of SPOP on cervical cancer cell proliferation.
RESULTS: As part of our study, we assessed that SPOP was anomalously expressed in kinds of cancers, related to clinical stage, and associated with clinical outcomes. Meanwhile, SPOP also played a crucial role in the tumor microenvironment (TME) and immune function.
CONCLUSIONS:Our study recovered the potential prognostic and immunological role of SPOPin multi-type cancers, and SPOP may be a tumor suppressor in cervical cancer.
Attitudes, practices, and barriers towards Medical Electronic Education During COVID-19 Pandemic among medical students in Syrian private university (SPU)- a cross-sectional Study.
Authors: Safiah Mhd homam , Alchallah Mhd Obai , ElHomsi Muhammad Omar , Ashabi Khaled Kalalib AL , Ataya Sham , Alolabi Homam , Mohsen Fatema , Nahas Louei Darjazini 
Published: August 2023

Abstract

Background
COVID-19 pandemic promptly and severely affected all facets of life. Education is one of the most significant areas that has been affected, what urgently needs remedies to make up for the educational loss. E- learning is one of the most common replacement methods for traditional education during the lockdown. This study aims to assess the attitudes, practices, and barriers of E- learning among medical students.
Methods
A cross-sectional study using convenience sampling was conducted at the faculty of medicine, at the Syrian Private University (SPU) in Damascus, Syria on (6/20/2021), after the first wave of the coronavirus pandemic. Data were collected through self-administered questionnaire.
Results
Of 519 students, 233 (44.9%) showed a positive attitude toward E-learning in general, while the most showed negative attitude 286 (55.1%). Regarding socio-demographics, on analysis, Academic year (p = 0.006), Housing (p = 0.001), and having financial problems (p = 0.014) were significantly associated with attitude. Most good e- learning practices correlated with positive attitude. Difficulty adjusting learning style, lack of technical skills, Poor communication with educators, no device or limited access, and limited space conductive for studying have statistically significant associated with having a positive attitude
Conclusion
our results indicate an acceptable positive attitude toward E-learning among medical students. However, E-learning still faces several challenges, particularly in developing nations with poor incomes. Some challenges, including social isolation, could be challenging to overcome. Despite this, developing non-traditional educational options is crucial in situations of crisis.
Changes of immune microenvironment in head and neck squamous cell carcinoma in 3D-4-culture compared to 2D-4-culture
Authors: Xiao Jian , Song Yexun , Gao Ru , You Mingyang , Deng Changxin , Tan Guolin , Li Wei 
Published: August 2023

Abstract
Background
The immune system plays a crucial role in initiating, progressing, and disseminating HNSCC. This study aims to investigate the differences in immune microenvironments between 2D-4-culture and 3D-4-culture models of head and neck squamous cell carcinoma (HNSCC) cells (FaDu), human fibroblasts (HF), human monocytes (THP-1), and human endothelial cells (HUVEC).
Methods
For the 3D-4-culture model, FaDu:HF: THP-1 (2:1:1) was inoculated in an ultra-low attachment culture plate, while HUVEC was placed in a transwell chamber. The ordinary culture plate was used for the 2D-4-culture model. Tumor-associated macrophage markers (CD163), tumor-associated fibroblast markers (FAP), and epithelial-mesenchymal transition (EMT) were detected by western blot. Inflammatory cytokines (IL-4, IL-2, CXCL 10, IL-1 β, TNF-α, CCL 2, IL-17 A, IL-6, IL-10, IFN-γ, IL-12 p 70, CXCL 8, TGFβ1) in the supernatant were measured by flow cytometry. HUVEC migration was observed under a microscope. The 3D spheres were stained and observed with a confocal microscope. CCK8 assay was used to detect the resistance of mixed cells to cisplatin in both 2D-4-culture and 3D-4-culture.
Results
After three days of co-culture, the 3D-4-culture model showed increased expression levels of CD163 and FAP proteins (both P < 0.001), increased expression of E-cadherin protein and N-cadherin protein expression (P < 0.001), decreased expression of vimentin (P < 0.01) and Twist protein (P < 0.001). HUVEC migration significantly increased (P < 0.001), as did the concentrations of IP-10, MCP-1, IL-6, and IL-10 (all P < 0.001). Confocal microscopy showed that 3D-4 co-culture formed loose cell clusters on day 1, which gradually became a dense sphere surrounded by FaDu cells invading the inside. After co-culturing for 24h, 48h, and 72h, the resistance of mix cells to cisplatin in 3D-4-culture was significantly higher than in 2D-4-culture (P < 0.01 for all).
Conclusion
Compared to 2D-4-culture, 3D-4-culture better simulates the in vivo immune microenvironment of HNSCC by promoting fibroblast transformation into tumor-associated fibroblasts, monocyte transformation into tumor-associated macrophages, enhancing endothelial cell migration ability, partial EMT formation in HNSCC cells, and is more suitable for studying the immunosuppressive microenvironment of HNSCC.
Genome-wide identification and characteristic analysis of ETS gene family in blood clam Tegillarca granosa
Authors: Jin Hongyu , Zhang Weiwei , Liu Hongxing , Bao Yongbo 
Published: August 2023

Abstract
Background
ETS transcription factors, known as the E26 transformation-specific factors, assume a critical role in the regulation of various vital biological processes in animals, including cell differentiation, the cell cycle, and cell apoptosis. However, their characterization in mollusks is currently lacking.
Results
The current study focused on a comprehensive analysis of the ETS genes in blood clam Tegillarca granosa and other mollusk genomes. Our phylogenetic analysis revealed the absence of the SPI and ETV subfamilies in mollusks compared to humans. Additionally, several ETS genes in mollusks were found to lack the PNT domain, potentially resulting in a diminished ability of ETS proteins to bind target genes. Interestingly, the bivalve ETS1 genes exhibited significantly high expression levels during the multicellular proliferation stage and gill tissues. Furthermore, qRT-PCR results showed that Tg-ETS-14 (ETS1) is significant upregulation in the high total hemocyte counts (THC) population of T. granosa, suggesting it assumes a significant function in stimulating hemocyte proliferation.
Conclusion
Our study significantly contributes to the comprehension of the evolutionary aspects concerning the ETS gene family, while also providing valuable insights into its role in fostering hemocyte proliferation across mollusks.
Adaptation and Study Protocol for Harvest for Health Together Arizona: a Mentored Community Garden Intervention for Survivors of Cancer
Authors: Skiba Meghan B , Miller Dylan , Stratton Delaney B , Hall Caitlyn A. , Filer Parker , McKenna Sharon , Blair Cindy K. , Demark-Wahnefried Wendy 
Published: August 2023

Abstract
Background
Current health behavior recommendations for skin cancer prevention, treatment, and survivorship are the same for survivors of other cancers; they include eating a healthy diet, being physically active, maintaining a healthy weight, and minimizing ultraviolet (U.V.) exposure. Harvest for Health is a home-based mentored gardening intervention for cancer survivors and was successful in Alabama and New Mexico. We adapted Harvest for Health for implementation in Arizona as a community-based intervention and will pilot test for feasibility, acceptability, and appropriateness.
Methods
Harvest for Health Together Arizona (H4H2-AZ) is a six-month single-arm intervention of mentored community-based vegetable gardening. H4H2-AZ will be piloted among 30 skin cancer survivors. Participant cohorts are aligned with the start of growing seasons (e.g., Spring, Monsoon, Fall). Primary outcomes will be feasibility, acceptability, and appropriateness of the intervention. Secondary outcomes are to explore the effects on cancer preventive health behaviors (dietary quality, physical activity, sedentary time, U.V. exposure, and sun protective habits) and health-related quality of life (HR-QOL). Stakeholder-informed adaptations for Arizona included updating intervention materials to be relevant to the arid desert environment, emphasizing the importance of sun safety in cancer survivorship, and shifting from a home-based to a community-based delivery model. Participants were matched to an individual 30 square foot community garden plot and Master Gardener Mentor. Original intervention components retained are: 1) Master Gardeners deliver the intervention providing one-to-one mentorship and 2) gardening materials and supplies provided.
Discussion
This pilot single-arm intervention will determine feasibility, acceptability, and appropriateness of an evidence-based adapted mentored community gardening intervention for survivors of skin cancer. If successful, the intervention could be widely implemented throughout existing Master Gardener programs and community garden networks for survivors of other cancers.
Trial registration:
ClinicalTrials.gov identifier: NCT05648604. Trial registered on December 13, 2022.
The Dynamic Impact of Digital Finance on Green Technology Innovation: Evidence Enterprise-level Empirical Data in China
Authors: Wang Jie , Wang Jun 
Published: August 2023

Abstract
The Digital finance generated by technology empowerment profoundly affects the innovative behavior of micro-enterprises. To estimate how digital finance influences the quantity and quality of green technological innovation, this paper introduces digital finance into endogenous economic growth model, further conducts correlation analysis with the combination of fixed effects model (FE) and panel threshold model (PTM). Moreover, this paper investigates the action mechanism from the perspective of financial constraint and financial cost. The empirical results indicate that digital finance improves the quantity and quality of green technological innovation significantly. Compared with the coverage of digital finance and digital services, the impact of usage depth on green innovation accumulates dynamically superimposed in the long term. The digital finance contributes to correct the mismatch of financial resources, further induces inclusive green innovation in the dimension of ownership, growth cycle and enterprise scale. Mechanism analysis shows that the relief of financial constraint and the reduction of financial cost are the main paths for digital finance to drive green technology innovation. With the different thresholds, The dynamic evolution process of green innovation driven by digital finance is characterized by "first quantitative change and then qualitative change". This paper suggests that, the integration of technological innovation and digital financial services needs to build an inclusive digital financial service system, cultivate diversified financial formats and improve the market environment of digital financial services.
JEL classification: G2; Q32
ICAM2 as a novel prospective tumor suppressor induced by ERG promotes ubiquitination-mediated RDX degradation to inhibit gastric cancer tumorigenicity and metastasis
Authors: Tang xiaocheng , Huang jintuan , Jiang yingming , Qiu jun , Li tuoyang , Li weiyao , Chen zijian , Huang zhenze , Yu xihu , Yang tao , Ji xiang , Tan rongchang , Lv li , Yang Zuli , Chen hao 
Published: August 2023

Abstract
Background
Gastric cancer (GC) is a fatal cancer with unclear pathogenesis. In this study, we explored the function and potential mechanisms of intercellular adhesion molecule 2 (ICAM2) in the development and advancement of GC.
Methods
Quantitative real-time polymerase chain reaction (qRT-PCR) and Western blotting were performed to quantify ICAM2 expression in harvested GC tissues and cultured cell lines. Immunohistochemical analyses were conducted on a GC tissue microarray to quantify ICAM2 expression and explore its implication on the prognosis of GC patients. In vitro experiments were carried out to reveal the biological functions of ICAM2 in GC cell lines. Further, in vivo experiments were conducted using xenograft models to assess the impact of ICAM2 on GC development and metastasis. Western blot, immunofluorescence, immunoprecipitation, luciferase assay, chromatin immunoprecipitation, and ubiquitination analysis were employed to investigate the underlying mechanisms.
Results
ICAM2 expression was downregulated in GC, positively correlating with advanced T stage, distant metastasis, advanced clinical stage, vessel invasion, and shorter patient survival time. ICAM2 overexpression suppressed the proliferation, migration, invasion, metastasis of GC cells as well as their ability to form tumors, whereas ICAM2 knockdown yielded opposite results. Erythroblast transformation-specific-related gene (ERG) as a transcription factor promoted the transcription of ICAM2 by binding to the crucial response element localized within its promoter region. Further analysis revealed that ICAM2 reduced radixin (RDX) protein stability and expression. In these cells, ICAM2 bound to the RDX protein to promote the ubiquitination and degradation of RDX via NEDD4 Like E3 Ubiquitin Protein Ligase (NEDD4L), and this post-translational modification resulted in the inhibition of GC.
Conclusions
In summary, this study demonstrates that ICAM2, which is induced by ERG, suppresses GC progression by enhancing the ubiquitination and degradation of RDX in a NEDD4L-dependent manner. Therefore, these results suggest that ICAM2 is a potential prognostic marker and a therapeutic target for GC.
Measuring mood: A comparison of the I-PANAS-SF and Affective Well-Being scales
Authors: Yount Garret , Balan-Artley Ema , Delorme Arnaud , Radin Dean , Carpenter Loren , Wahbeh Helané 
Published: August 2023

Abstract

Objective: Two well-being scales were administered to participants attending personal transformational workshops; the International Positive and Negative Affect Schedule Short-form (I-PANAS-SF) and the Affective Well-Being Scale. Data were collected before and after workshops occurring between March 17, 2018, and November 4, 2020, during which 1,175 unique records were collected. A previous publication reports on changes in I-PANAS-SF results, comparing before and after the workshops. This study compares the I-PANAS-SF results with those of the Affective Well-Being Scale obtained from these same study participants.
Results: The two well-being scales yielded similar results. While the I-PANAS-SF scale produced more nuanced results, the additional time required must be balanced against the value of the additional information gained.
Chemical constituents and anti-inflammatory, antinociceptive, and antioxidant activities of Salvia melissiflora Benth. aerial parts
Authors: Oliveira Cristhian Santos , Barbosa Felipe Lukacievicz , Ehrenfried Carlos Augusto , Rodrigues Tayná Eloyane Barreto , Salvador Marcos José , Zampronio Aleksander Roberto , Stefanello Maria Élida Alves 
Published: August 2023

Abstract
Chromatographic fractionation of dichloromethane (EDSM) and ethanol (EESM) extracts of Salvia melissiflora Benth. aerial parts led to the identification of five known compounds through analyses of NMR data (1D and 2D), and comparison with literature data: oleanolic acid (1), ursolic acid (2), ent-(5R,9R)-15,16-epoxy-10S-hydroxycerodan-3,13(16),14-triene-17,12S;18,19-diolide (melissiflorine, 3), 7-epi-salvianduline A (4) and rosmarinic acid (5). The anti-inflammatory and antinociceptive activity of EESM and EDSM were evaluated in mice. The oral administration of EESM reduced the second phase of formalin-induced nociception, the lipopolysaccharide (LPS)-induced hyperalgesia and the carrageenan-induced edema in mice. The oral administration of EDSM also reduced LPS-induced hyperalgesia without altering motor performance of the animals. The anti-inflammatory and anti-nociceptive effects are probably related to the presence of compounds 5 and 1, and 2, respectively. The antioxidant activity of the extracts was evaluated using the ORAC method. The EESM extract showed activity (TE relative: 2845.2 µmol TE g− 1), which can be attributed to the major presence, in this extract, of compound 5, which showed an antioxidant capacity comparable to that of caffeic acid.
Factors associated with the development of venous thromboembolic disease due to failed thromboprophylaxis.
Authors: Perez Santiago Grillo , Talero Paula Andrea Ruiz , Velandia Oscar Mauricio Munoz 
Published: August 2023

Abstract
Introduction: Available evidence to identify factors independently associated with failed thromboprophylaxis (FT) in medical patients is insufficient. The present study seeks to evaluate in hospitalized patients, which clinical factors are associated for the development of FT.
Materials and methods: A case-control study nested to a historical cohort, comparing patients who developed failed thromboprophylaxis (cases) with those who did not (controls). Univariate and multivariate regression analysis was performed to define the factors associated with FT.
Results: We collected 204 cases and 408 controls (52.4% men, median age 63 years). Seventy-eight point four percent had indication for medical hospitalization. The most frequent thromboprophylaxis scheme was enoxaparin. In the failed thromboprophylaxis group, most of the embolic events corresponded to pulmonary thromboembolism (53.4%). Among cases, BMI was higher (26.3 vs 25 kg/m2, p<0.001), as was the proportion of patients with leukocytosis >13,000 (27% vs 18.9%, p:0.22), and the proportion who required intensive care management (48% vs 24.8%,p<0.001). Factors independently associated with FT were BMI (OR1.04;95%CI 1.00-1.09, p:0.39), active cancer (OR:1.63;95%IC 1.03-2.57,p:0.04), leukocytosis (OR:1.64;95%CI 1.05 - 2.57, p0.03) and ICU requirement (OR:3.67;95%CI 2.31-5.83,p<0.001).
Conclusion: Our study suggests that the development of failed thromboprophylaxis is associated with elevated BMI, active cancer, leukocytosis and ICU requirement. Future studies should evaluate whether there is benefit in adjusting the thromboprophylaxis schedule in medical patients with one or more of these factors.
Home-based self-management for sedentary individuals with mild walking disability after stroke: protocol for a randomized pilot study
Authors: Alvarenga Maria Tereza Mota , Ada Louise , Preston Elisabeth , Caetano Lívia Cristina Guimarães , Teixeira-Salmela Luci Fuscaldi , Scianni Aline Alvim 
Published: August 2023

Abstract
Background
A Phase I study showed that is feasible to implement a home-based self-management program aimed at increasing physical activity in individuals after stroke with mild impairments in Brazil. The next step is to test this program against a control group in order to provide a power analysis for a fully-powered Phase III clinical trial.
Methods
A Phase II pilot randomized clinical trial with concealed allocation, blinded measurement, and intention-to-treat analyses will be carried out. The inclusion criteria will be: individuals who had suffered a stroke, in the acute or subacute phase, with mild impairments, sedentary, and with no significant language impairment. The individuals will be randomly allocated to the experimental or control group. The experimental group will receive 6 sessions of a home-based self-management program, based on behaviour change techniques, over 11 weeks. The control group will receive education about stroke, orientation regarding the importance of practicing physical activity after a stroke and usual care. A total of 24 participants will be recruited. The primary outcome will be physical activity, measured through steps taken per day by an activity monitor (Actigraph wGT3X-BT, Pensacola, FL, USA). Secondary outcomes will be cardiovascular risk (body mass index, waist circumference, and blood pressure), depressive symptoms (Geriatric Depression Scale), walking ability (6-Minute Walk Test and 10-Meter Walk Test), exercise self-efficacy (Self-Efficacy for Exercise scale), social participation (Stroke Impact Scale) and quality of life (EuroQual-5D). All outcomes will be measured at baseline (Week 0), post-intervention (Week 12), and follow-up (Week 24). This pilot clinical trial was registered online at Clinical Trials under number NCT05461976 in 4th April, 2022.
Discussion
If beneficial, this Phase II pilot randomized trial will provide data to plan a fully powered future Phase III clinical trial aimed at verifying the efficacy of this program to promote physical activity after stroke.
Trial registration:
Clinical Trials NCT05461976.