bluuebunny/crossref_metadata_2025_split huggingface dataset:
Pomegranate juice consumption by patients under medication for addiction treatment as regulator of craving and blood redox status: The study protocol of a randomized control trial (the NUTRIDOPE study)
Authors: Leventelis Christonikos , Tasoulis Sotiris , Kouretas Demetrios , Metsios George S. , Veskoukis Aristidis S. 
Published: August 2023

Abstract
Background
Buprenorphine and methadone are considered the “gold standard” medication for addiction treatment (MAT) for patients with opioid use disorders (OUDs). However, they may cause side effects promoting craving. Pomegranate is a natural substance that contains antioxidant polyphenolic compounds, which have been associated with craving reduction. Aim: The NUTRIDOPE (NUTRItion-driven Detoxification of OPioid addicted patiEnts) study aims to investigate the role of pomegranate juice consumption by opioid patients under buprenorphine and methadone on craving, as the primary outcome, and biopsychosocial parameters.
Methods
NUTRIDOPE is a randomized control trial with repeated measures. The participants, who will be patients with OUDs attending rehab programs in the Greek Organization Against Drugs, will be randomly divided into the experimental and the control groups and both groups will be further stratified into two subgroups, i.e., methadone and buprenorphine, according to the maintenance treatment program they attend. Pomegranate juice will be administered to the participants of the experimental group, whereas their counterparts in the control group will not consume any similar beverage. The administration regimen will be 250 ml, seven days/week, four months.
Results
Craving, as well as physiological (sleep, constipation, fatigue), psychosocial (depression, mood state, quality of life) and biochemical (i.e., blood redox status and inflammation) parameters will be evaluated.
Conclusions
NUTRIDOPE is a hypothesis-driven, evidence-based, multifactorial project that proposes a nutrition-based solution towards craving reduction for opioid patients under MAT, potentially assisting towards their successful rehab and societal reintegration.
ClinicalTrials.gov Identifier:
NCT05861544
An iron fist in a velvet glove: The cooperation of a novel pyoverdine from Pseudomonas donghuensis P482 (PVDP482) with 7-hydroxytropolone is pivotal for its antibacterial activity.
Authors: Jafra Sylwia , Jabłońska Magdalena , Maciąg Tomasz , Matuszewska Marta , Borowicz Marcin , Prusiński Michał , Żmudzisńska Wioletta , Theil Marcel , Czaplewska Paulina , Krzyżanowska Dorota M. , Czajkowski Robert 
Published: August 2023

Abstract
Pseudomonas donghuensis P482 exhibits broad antimicrobial activity against phytopathogens, including the soft rot bacteria of the Dickeya genus. Here, we report that under limited nutrient availability, the antibacterial activity of P. donghuensis P482 against Dickeya solani requires the reciprocal action of two iron scavengers: 7-hydroxytropolone (7-HT) and a newly characterised pyoverdine (PVDP482) and is quenched in the iron-augmented environment. Further, we show that the biosynthesis of pyoverdine and 7-HT is metabolically coordinated, and the functional BV82_4709 gene involved in 7-HT synthesis is pivotal for expressing the BV82_3755 gene, essential for pyoverdine biosynthesis and vice versa. The synthesis of both scavengers is under the control of Gac/Rsm, but only PVD is controlled by Fur. The isoelectric focusing profile of the P482 siderophore differs from that of the other Pseudomonas spp. tested. This finding led to the unveiling of the chemical structure of the new pyoverdine PVDP482. To summarise, the antibacterial activity of P. donghuensis P482 is attributed to 7-HT and PVDP482, varies depending on the nutrient and iron availability, highlighting the importance of these factors in the competition between P482 and D. solani.
Influence of crystallographic anisotropy on the electrical conductivity of apatite at high temperatures and high pressures
Authors: Hu Ziming , Dai Lidong , Hu Haiying , Sun Wenqing , Wang Mengqi , Jing Chenxin , Yin Chuanyu , Luo Song , Lai Jinhua 
Published: August 2023

Abstract
The electrical conductivity of apatite single crystals along three main crystalline directions was measured in situ using a YJ-3000t multi-anvil apparatus and a combined system consisting of the impedance/gain-phase analyzer (Solartron 1260) and dielectric interface (Solartron 1296) at 973–1373 K and 1.0–3.0 GPa. The obtained results indicate that the relationship between the electrical conductivity and temperature conforms to the Arrhenius relation. At 2.0 GPa, the electrical conductivity of apatite with relatively high activation enthalpies of 1.92–2.24 eV shows a significant anisotropy with an extremely high anisotropic degree (τ = ~8–16) value. For a given [001] crystallographic orientation, the electrical conductivity of apatite slightly decreases with increasing pressure, and its corresponding activation energy and activation volume of charge carriers are 2.05 ± 0.06 eV and 9.31 ± 0.98 cm3/mol, respectively. All of these observed anomalously high activation enthalpy and positive activation volume values suggest that the main conduction mechanism is related to the monovalent fluorine anion at high temperature and high pressure. Furthermore, three representative petrological average schemes, including the parallel, Hashin-Shtrikman upper bound, and average models were selected to establish the functional relation for the electrical conductivity of the phlogopite-apatite-peridotite rock system along with the volume percentages of apatite ranging from 1 to 10% at 973–1373 K and 2.0 GPa. For a typical Hashin-Shtrikman upper bound model, the electrical conductivity-depth profile for peridotite containing the 10% volume percentage of apatite was successfully constructed in conjunction with our acquired anisotropic electrical conductivity results and available temperature gradient data (11.6 and 27.6 K/km) at depths of 20–90 km. Although the presence of apatite in peridotite cannot explain the high-conductivity anomalies in western Junggar of Xinjiang autonomous region, it may provide a reasonable constraint on those of representative apatite-rich areas.
Znucalite, the only known zinc uranyl carbonate: Its crystal structure and environmental implications
Authors: Steciuk Gwladys , Majzlan Juraj , Rohlíček Jan , Škoda Radek , Sejkora Jiří , Plášil Jakub 
Published: August 2023

Abstract
Znucalite is a zinc uranyl-carbonate mineral that was until recently only partially characterized with a formula originally given as Zn12Ca(UO2)(CO3)3(OH)22·4H2O, with an unknown crystal structure and ambiguous symmetry determinations. We have reinvestigated this mineral using three-dimensional electron diffraction (3D ED) and powder X-ray diffraction and revealed for the first time its structural details. Znucalite is unambiguously monoclinic, P21/m, with a = 10.722(2) Å, b = 6.259(1) Å, c = 25.355(1) Å, β = 101.13(1)°, and V = 1669.54(9) Å3. The structure refinement of the 3D ED data using the dynamical approach (Robs = 0.1594 for 3579 observed reflections and 244 parameters) provided the following structure model. Znucalite possesses a layered structure, with a [Zn10(OH)14(CO3)2] double sheet (with Zn2+ both in octahedra and tetrahedra), which is connected to a thick interlayer that hosts U 6+, Ca2+, and H2O molecules. The linkage between structural units and the interlayer occurs via the vertices of ZnO4 tetrahedra protruding from the sheet. In the interlayer, differences in ordering between U and Ca take place and likely cause the difficulties encountered during the attempts to solve the structure. The refined structural formula of znucalite, Zn10Ca0.828[UO2]0.828[CO3]4(OH)15.312(H2O)5.484, corresponds well to the composition obtained from the electron-microprobe analyses, (Zn9.84Al0.16)Σ10.00 Ca0.83(UO2)0.80[(CO3)3.96(SO4)0.04]Σ4.00(OH)15.42(H2O)5.48. Raman spectroscopy evidenced the presence of several non-equivalent CO3 groups, as well as OH and H2O. The U-O bond lengths obtained from the stretching frequencies of UO22+ vibrations are in line with the structural model. A discussion on the environmental importance of znucalite is appended, based on geochemical calculations with an estimate of the solubility product for this mineral.
Ofatumumab and Early Immunological Cells Subset Characterization in
Naïve Relapsing Multiple Sclerosis Patients: A Real-World Study
Authors: D’Amico Emanuele , Zanghì Aurora , Fantozzi Roberta , Centonze Diego , Avolio Carlo 
Published: August 2023

Background:
Ofatumumab (OFA) is a fully human anti-CD20 monoclonal antibody administered
with a 20 mg subcutaneous monthly dosing regimen.

Methods:
Inclusion criteria were patients: 1) aged 18-55; 2) with a confirmed diagnosis of relapsing
Multiple Sclerosis (RMS), per the revised 2010 McDonald criteria; 2) who started OFA according to
Italian Medicines Agency prescription rules and within 12 months from the RMS diagnosis; 3) naïve
to any disease-modifying therapy. The primary outcome was to offer an overview of cellular subsets
of RMS naïve patients (time 0) and then after 4 weeks (time 1) and 12 weeks (time 2) on therapy with
OFA in a real-world setting.

Results:
Fifteen patients were enrolled. CD3+ T cell frequencies were higher at time 1 (%80.4, SD
7.7) and time 2 (%82.6, SD 5.8) when compared to time 0 (%72.4, SD 9.8), p = .013. B naïve cells
were barely detectable in the OFA group at time 1 (%0.4, SD 0.5) and 2 (%1.4, SD 2.9) when compared
to time 0 (%11.5, SD 3.8), p < .001.

Conclusion:
The progressive and increasing use of anti-CD20 drugs imposes the need for larger, prospective,
real-world, long-term studies to characterize further immunophenotypes of patients with
RMS treated with OFA.
Identification of Methamphetamine and Amphetamine in Drug Abuse
Urine Sample using Multi-drug Rapid Test and Gas Chromatography-
Mass Spectrometry (GC-MS) at the Centre of Drug Testing Laboratory,
National Narcotics Board Republic of Indonesia
Authors: Sandy Kurnia , Rahmania Tesia Aisyah , Arfiyani Eri 
Published: August 2023

Introduction:
Drug abuse is a real threat that threatens the Indonesian nation in the form of
non-military threats. The second most abused illicit substance in the world is methamphetamine
(MA). Most of the methamphetamine users in the world live in East and Southeast Asia. Methamphetamine
is a powerful stimulant of the central nervous system (CNS) that can make someone feel an increase
in sympathomimetic.

Methods:
First, the preliminary test was conducted using a BioCare multi-drug rapid test and a specific
methamphetamine rapid test that works based on the Lateral Flow Assay (LFA) principle. Second, the
sample preparation used Liquid-Liquid Extraction (LLE). Third, GC-MS confirmed the test using GCMS
Shimadzu-QP2020NX-GC-2030 (HP-5MS column, Length 30 m, diameter 0.25 m, film 0.25).

Result and Discussion:
The Preliminary test shows that the urine is positive and contains methamphetamine
and amphetamine. Based on the sample chromatogram, There are 2 peaks, which are methamphetamine
and amphetamine compounds it can be seen by the retention time is 5.560 minutes
(methamphetamine) a similarity index of 98% to methamphetamine compounds, and 4.885 minutes
(amphetamine) with a similarity index of 96% to amphetamine compounds.

Conclusion:
The urine samples with code 210DGB from methamphetamine users were detected due
to the presence of methamphetamine and amphetamine, which is the metabolite of methamphetamine.
Isolation and Biochemical Characterization of Ananassains, Cysteine Peptidases
from the Fruits of Ananas ananassoides
Authors: Okayama Adriana , Cabral Hamilton , Gustavo Orlando Bonilla-Rodriguez 
Published: August 2023

Aims:
This work performed a preliminary characterization of two new peptidases from
Ananas ananassoides.

Background:
Proteolytic enzymes, also known as peptidases, are found in all living things and play
critical physiological roles in metabolism and cellular regulation. They account for roughly 60% of the
enzymes used in industry and have high proteolytic activity, such as papain from Carica papaya latex
and stem and fruit bromelains from the edible pineapple Ananas comosus.

Objective:
The wild pineapple Ananas ananassoides contains proteolytic enzymes, which motivated
this study due to the potential applications of this type of enzyme.

Methods:
The fruit and stem of A. ananassoides were blended, clarified, and purified using chromatography
(SP-Sepharose and Sephadex G-50). The molecular mass was determined using mass spectrometry
(M.S.), and the N-terminal sequences were obtained and compared to other Bromeliaceae
proteases. Fluorogenic substrates were used to determine the kinetic parameters.

Results:
As determined by M.S., the fruit and stem contain cysteine-peptidases with Mr of 27,329.6
and 23,912.5 Da, respectively, values that are very similar to those found in edible pineapple bromelains.
Despite Mr and carbohydrate composition differences, both proteases have similar optimum pH
values. They have similar temperature effects, though the stem protease is more thermally stable. Both
proteases have a stronger preference for hydrophobic, polar, and basic residues. Both proteases hydrolyzed
substrates containing polar and basic residues.

Conclusion:
A comparison of the N-terminal sequences (AVPQIIDW for fruit ananassains and AVPEIIDW
for stem ananassains) reveals a high degree of homology when compared to other Bromeliaceae
proteases such as papain.
Natural Flavonoids as an Intervention for Hepatic Encephalopathy: Preclinical
Evidence-based Review
Authors: Singh Niraj Kumar , Solanki Ashutosh 
Published: August 2023

Abstract:
Hepatic encephalopathy (HE) is a serious neuropsychiatric disorder caused in patients
with both; acute and chronic liver diseases, which consists of various complications ranging from
cognitive impairment, disorientation, confusion, and coma. The available therapies mainly focus on
decreasing ammonia levels either through increasing its elimination or decreasing its production,
some medications may subside the duration and limit the consequences of HE, but there is no complete
available treatment for HE-like manifestation. Thus, there is a need to explore new pharmacotherapy
for the treatment and management of HE. Flavonoids are polyphenolic compounds easily
found in vegetables, fruits, flowers, beverages, and plants based foods. In modern research, flavonoids
have gained attention due to their broad pharmacological properties, like anti-oxidant, antiviral,
anti-inflammatory, cardioprotective, cytoprotective, and neuroprotective activity. Several preclinical
studies suggest that various flavonoids have a potential therapeutic role in a variety of metabolic-
related neurological disorders, including HE. This review focuses on all pre-clinical reports
that highlight the neuroprotective potential of natural flavonoids for the management of HE. Based
on numerous pre-clinical studies and taking into account the therapeutic effects of natural flavonoids,
the present study illustrates the cellular and molecular mechanisms responsible for the potential
role of natural flavonoids as pharmacotherapy for the management and treatment of HE.
Do Personality Traits Predict Mental Well-being in the Context of
Erroneous Subjective Estimation of Smartphone Screen Time?:
Findings from a Cross-sectional Observational Study Among
College Students
Authors: Balhara Yatan Pal Singh , Singh Swarndeep , Ganesh Ragul , Kattula Dheeraj , Abhijita Bandita , Gupta Amulya , Gupta Abhinav 
Published: August 2023

Background:
The current article reports on the findings from a cross-sectional survey
conducted among college students on a subjectively reported and objectively measured
smartphone screen time (ST) and its relation to mental well-being.

Methods:
We explored the magnitude of discrepancy between subjectively reported and objectively
measured smartphone ST. Moreover, we assessed the interaction of mental well-being
with personality traits among subjects with a discrepancy between the subjectively reported
and objectively measured smartphone ST. The mental health of study participants was assessed
using the WHO well-being index. Personality was assessed using the Big Five Inventory (BFI)-
10. A total of 202 students shared screenshots of the phone ST function and were included in
the analysis.

Results:
A total of 145 (71.8%) participants underestimated their daily smartphone ST, whereas
56 (27.7%) of them overestimated ST. In the regression analysis, the predicted odds of poor
mental well-being were 1.43 times greater for subjects with higher neuroticism scores in the
overall sample. Moreover, the predicted odds of poor mental well-being were 1.593 times
greater for subjects with higher neuroticism scores among the study subjects who underestimated
their ST.

Conclusions:
The findings of the current study suggested that the magnitude of discrepancy
between the subjectively estimated and objectively estimated ST varies across college students,
with the discrepancy being significantly higher among those who overestimated their ST. The
predicted odds of poor mental well-being were about one and a half times greater for subjects
with higher neuroticism scores among college students who underestimated their ST.
Effect of Benign Biopsy Findings on an Artificial Intelligence–Based Cancer Detector in Screening Mammography: Retrospective Case-Control Study
Authors: Zouzos Athanasios , Milovanovic Aleksandra , Dembrower Karin , Strand Fredrik 
Published: August 2023

Background
Artificial intelligence (AI)–based cancer detectors (CAD) for mammography are starting to be used for breast cancer screening in radiology departments. It is important to understand how AI CAD systems react to benign lesions, especially those that have been subjected to biopsy.

Objective
Our goal was to corroborate the hypothesis that women with previous benign biopsy and cytology assessments would subsequently present increased AI CAD abnormality scores even though they remained healthy.

Methods
This is a retrospective study applying a commercial AI CAD system (Insight MMG, version 1.1.4.3; Lunit Inc) to a cancer-enriched mammography screening data set of 10,889 women (median age 56, range 40-74 years). The AI CAD generated a continuous prediction score for tumor suspicion between 0.00 and 1.00, where 1.00 represented the highest level of suspicion. A binary read (flagged or not flagged) was defined on the basis of a predetermined cutoff threshold (0.40). The flagged median and proportion of AI scores were calculated for women who were healthy, those who had a benign biopsy finding, and those who were diagnosed with breast cancer. For women with a benign biopsy finding, the interval between mammography and the biopsy was used for stratification of AI scores. The effect of increasing age was examined using subgroup analysis and regression modeling.

Results
Of a total of 10,889 women, 234 had a benign biopsy finding before or after screening. The proportions of flagged healthy women were 3.5%, 11%, and 84% for healthy women without a benign biopsy finding, those with a benign biopsy finding, and women with breast cancer, respectively (P<.001). For the 8307 women with complete information, radiologist 1, radiologist 2, and the AI CAD system flagged 8.5%, 6.8%, and 8.5% of examinations of women who had a prior benign biopsy finding. The AI score correlated only with increasing age of the women in the cancer group (P=.01).

Conclusions
Compared to healthy women without a biopsy, the examined AI CAD system flagged a much larger proportion of women who had or would have a benign biopsy finding based on a radiologist’s decision. However, the flagging rate was not higher than that for radiologists. Further research should be focused on training the AI CAD system taking prior biopsy information into account.
An eHealth App (CAPABLE) Providing Symptom Monitoring, Well-Being Interventions, and Educational Material for Patients With Melanoma Treated With Immune Checkpoint Inhibitors: Protocol for an Exploratory Intervention Trial
Authors: Fraterman Itske , Wollersheim Barbara M , Tibollo Valentina , Glaser Savannah Lucia Catherina , Medlock Stephanie , Cornet Ronald , Gabetta Matteo , Gisko Vitali , Barkan Ella , di Flora Nicola , Glasspool David , Kogan Alexandra , Lanzola Giordano , Leizer Roy , Mallo Henk , Ottaviano Manuel , Peleg Mor , van de Poll-Franse Lonneke V , Veggiotti Nicole , Śniatała Konrad , Wilk Szymon , Parimbelli Enea , Quaglini Silvana , Rizzo Mimma , Locati Laura Deborah , Boekhout Annelies , Sacchi Lucia , Wilgenhof Sofie 
Published: August 2023

Background
Since treatment with immune checkpoint inhibitors (ICIs) is becoming standard therapy for patients with high-risk and advanced melanoma, an increasing number of patients experience treatment-related adverse events such as fatigue. Until now, studies have demonstrated the benefits of using eHealth tools to provide either symptom monitoring or interventions to reduce treatment-related symptoms such as fatigue. However, an eHealth tool that facilitates the combination of both symptom monitoring and symptom management in patients with melanoma treated with ICIs is still needed.

Objective
In this pilot study, we will explore the use of the CAPABLE (Cancer Patients Better Life Experience) app in providing symptom monitoring, education, and well-being interventions on health-related quality of life (HRQoL) outcomes such as fatigue and physical functioning, as well as patients’ acceptance and usability of using CAPABLE.

Methods
This prospective, exploratory pilot study will examine changes in fatigue over time in 36 patients with stage III or IV melanoma during treatment with ICI using CAPABLE (a smartphone app and multisensory smartwatch). This cohort will be compared to a prospectively collected cohort of patients with melanoma treated with standard ICI therapy. CAPABLE will be used for a minimum of 3 and a maximum of 6 months. The primary endpoint in this study is the change in fatigue between baseline and 3 and 6 months after the start of treatment. Secondary end points include HRQoL outcomes, usability, and feasibility parameters.

Results
Study inclusion started in April 2023 and is currently ongoing.

Conclusions
This pilot study will explore the effect, usability, and feasibility of CAPABLE in patients with melanoma during treatment with ICI. Adding the CAPABLE system to active treatment is hypothesized to decrease fatigue in patients with high-risk and advanced melanoma during treatment with ICIs compared to a control group receiving standard care. The Medical Ethics Committee NedMec (Amsterdam, The Netherlands) granted ethical approval for this study (reference number 22-981/NL81970.000.22).

Trial Registration
ClinicalTrials.gov NCT05827289; https://clinicaltrials.gov/study/NCT05827289

International Registered Report Identifier (IRRID)
DERR1-10.2196/49252