bluuebunny/crossref_metadata_2025_split huggingface dataset:
acorn: an R package forde novovariant analysis
Authors: Turner Tychele N. 
Published: April 2023

ABSTRACTBackgroundThe study ofde novovariation is important for assessing biological characteristics of new variation and for studies related to human phenotypes. Software programs exist to callde novovariants and programs also exist to test the burden of these variants in genomic regions; however, I am unaware of a program that fits in between these two aspects ofde novovariant assessment. This intermediate space is important for assessing the quality ofde novovariants and to understand the characteristics of the callsets. For this reason, I developed the R package acorn.Resultsacorn is an R package that examines various features ofde novovariants including subsetting the data by individual(s), variant type, or genomic region; calculating features including variant change counts, variant lengths, and presence/absence at CpG sites; and characteristics of parental age in relation tode novovariant counts.Conclusionsacorn is an R package that fills a critical gap in assessingde novovariants and will be of benefit to many investigators studyingde novovariation.
Non-Human Peptides Revealed in Blood Reflect the Composition of Small Intestine Microbiota
Authors: Arapidi Georgij P. , Urban Anatolij S. , Osetrova Maria S. , Shender Victoria O. , Butenko Ivan O. , Bukato Olga N. , Kuznetsov Alexandr A. , Saveleva Tatjana M. , Nos Grigorii A. , Ivanova Olga M. , Lopukhov Leonid V. , Laikov Alexander V. , Sharova Nina I. , Nikonova Margarita F. , Mitin Alexander N. , Martinov Alexander I. , Grigorieva Tatiana V. , Ilina Elena N. , Ivanov Vadim T. , Govorun Vadim M. 
Published: April 2023

AbstractThe previously underestimated effects of commensal gut microbiota on the human body are increasingly being investigated using omics. The discovery of active molecules of interaction between the microbiota and the host may be an important step towards elucidating the mechanisms of symbiosis. Here, we show that in the bloodstream of healthy people, there are over 900 peptides that are fragments of proteins from microorganisms which naturally inhabit human biotopes, including the intestinal microbiota. Absolute quantitation by multiple reaction monitoring has confirmed the presence of bacterial peptides in the blood plasma and serum in the range of approximately 0.1 nM to 1 μM. The abundance of microbiota peptides reaches its maximum about 5h after a meal. Most of the peptides correlate with the bacterial composition of the small intestine and are likely obtained by hydrolysis of membrane proteins with trypsin, chymotrypsin and pepsin — the main proteases of the gastrointestinal tract. The peptides have physicochemical properties allowing them selectively pass the intestinal mucosal barrier and resist fibrinolysis. Proposed approach to the identification of microbiota peptides in the blood may be useful for determining the microbiota composition of hard-to-reach intestinal areas and for monitoring the permeability of the intestinal mucosal barrier.
Design, synthesis, and evaluation of a mitoxantrone probe (MXP) for biological studies
Authors: Wallin Savanna , Singh Sarbjit , Borgstahl Gloria E. O. , Natarajan Amarnath 
Published: April 2023

AbstractMitoxantrone (MX) is a robust chemotherapeutic with well-characterized applications in treating certain leukemias and advanced breast and prostate cancers. The canonical mechanism of action associated with MX is its ability to intercalate DNA and inhibit topoisomerase II, giving it the designation of a topoisomerase II poison. Years after FDA approval, investigations have unveiled novel protein-binding partners, such as methyl-CpG-binding domain protein (MBD2), PIM1 serine/threonine kinase, RAD52, and others that may contribute to the therapeutic profile of MX. Moreover, recent proteomic studies have revealed MX’s ability to modulate protein expression, illuminating the complex cellular interactions of MX. Although mechanistically relevant, the differential expression across the proteome does not address the direct interaction with potential binding partners. Identification and characterization of these MX-binding cellular partners will provide the molecular basis for the alternate mechanisms that influence MX’s cytotoxicity. Here, we describe the design and synthesis of a MX-biotin probe (MXP) and negative control (MXP-NC) that can be used to define MX’s cellular targets and expand our understanding of the proteome-wide profile for MX. In proof of concept studies, we used MXP to successfully isolate a recently identified protein-binding partner of MX, RAD52, in a cell lysate pulldown with streptavidin beads and western blotting.Graphical abstract (Draft)HighlightsAn 8-step synthesis was used to generate a biotinylated-mitoxantrone probe (MXP).A pulldown of MXP demonstrated selectivity for RAD52, but not Replication Protein A.Western blot confirmed the identity of the isolated protein, RAD52.
Phosphatase to kinase switch of a critical enzyme contributes to timing of cell differentiation
Authors: Chong Trisha N. , Saurabh Saumya , Panjalingam Mayura , Shapiro Lucy 
Published: April 2023

AbstractCell differentiation is an essential biological process that is often subject to strict temporal regulation. The aquatic bacterium,Caulobacter crescentus, undergoes obligate differentiation from a swarmer cell to a stationary, replication-competent stalked cell, with each cell cycle. Here, we report that the switch from phosphatase to kinase activity of the histidine kinase PleC contributes to timing this differentiation event. We show that PleC PAS domain interaction with the polar scaffold protein PodJ localizes PleC to the cell pole and inhibitsin vivokinase activity. Upon PodJ degradation, released PleC switches to its kinase form and phosphorylates the PleD diguanylate cyclase, initiating the signaling pathway responsible for differentiation. While PodJ inhibits PleC kinase activity, it does not impact PleC phosphatase activity on DivK, which is required for pili biogenesis and flagellar rotation. Thus, PleC PAS domain interaction with PodJ regulates PleC subcellular localization, enzymatic activity, and the timing of cell differentiation, revealing that PAS domains affect enzymatic function on diverse substrates by relying on context dependent binding partners.
Gender heterogeneity in the boardroom and corporate sustainability performance of quoted manufacturing firms in Nigeria
Authors: Egbunike Chinedu Francis , Igbinovia Ikponmwosa Michael , Oranefo Chinyere Patricia , Iyoha Agbonrha-Oghoye Imas 
Published: April 2023

PurposePrior studies have shown that heterogeneity plays a crucial role in addressing soft issues linked to a firm’s corporate social responsibility stance. The purpose of this paper is to extend the prior literature on the effect of gender heterogeneity on environmental, social and economic performance dimensions, specifically, whether the female boardroom presence weakens or strengthens the performance along the three dimensions, commonly referred to as the corporate sustainability.Design/methodology/approachThe study from a positivist philosophy adopts a quantitative approach, and the final sample consisted of forty-six companies listed on the Nigerian Stock Exchange for the year 2011–2018. The final sample was a balanced panel of 344 firm years. The dependent variables were return on assets (ROA), environmental performance (ENV) and donations made for social causes (SOP). The ENV was measured using a content scoring system, with range of 1 to 5. The data were analysed using the fixed effects and GLM regression models. To further address the issue of endogeneity, a two-stage least squares regression was conducted.FindingsThe findings show a positive relationship between gender heterogeneity and ROA, which was also confirmed for the environmental performance index. However, the sign reversed in the SOP model and showed a negative relationship between gender heterogeneity and donations, the proxy for SOP. The results are in tandem with the stakeholder axioms that argue that commitment to other stakeholder groups strengthens firm performance in the long run.Research limitations/implicationsAn implication of this study is the fact that information availability has been rapidly escalating in the country, leading to rising social movements and civic unrest; therefore, corporations that face negative castigations may pay the huge price of product boycott and loss of market value.Originality/valueThe findings of this study provide additional insight into the influence of female boardroom presence on the environmental, social and economic performance of firms. The findings suggest the relevance of the resource dependence theory, especially from a developing country context, to ensure an improved corporate governance structure in Nigerian manufacturing firms.
Hybrid approach for parameter identification of the two-diode model of photovoltaic modules
Authors: Zhang Guoyu , Wang Honghua , Lu Tianhang , Wang Chengliang , Huang Yaopeng 
Published: April 2023

Purpose
Parameter identification of photovoltaic (PV) modules plays a vital role in modeling PV systems. This study aims to propose a novel hybrid approach to identify the seven parameters of the two-diode model of PV modules with high accuracy.

Design/methodology/approach
The proposed hybrid approach combines an improved particle swarm optimization (IPSO) algorithm with an analytical approach. Three parameters are optimized using IPSO, whereas the other four are analytically determined. To improve the performance of IPSO, three improvements are adopted, that is, evaluating the particles with two evaluation functions, adaptive evolutionary learning and adaptive mutation.

Findings
The performance of proposed approach is first verified by comparing with several well-established algorithms for two case studies. Then, the proposed method is applied to extract the seven parameters of CSUN340-72M under different operating conditions. The comprehensively experimental results and comparison with other methods verify the effectiveness and precision of the proposed method. Furthermore, the performance of IPSO is evaluated against that of several popular intelligent algorithms. The results indicate that IPSO obtains the best performance in terms of the accuracy and robustness.

Originality/value
An improved hybrid approach for parameter identification of the two-diode model of PV modules is proposed. The proposed approach considers the recombination saturation current of the p–n junction in the depletion region and makes no assumptions or ignores certain parameters, which results in higher precision. The proposed method can be applied to the modeling and simulation for research and development of PV systems.
Young consumers' green marketing orientation: role of customer citizenship behaviour in determining real estate purchase intention in India
Authors: Jacob Jobin , Chully Arun Antony , Godwin Benny J. , George Jossy P. 
Published: April 2023

Purpose
The purpose of this paper is to assess green marketing as an influential factor in the purchase of real estate. In this study, the consumer citizenship behaviour of young individuals will be studied with an assessment of real estate purchase intention, strategic green marketing orientation (GMO) and tactical GMO.

Design/methodology/approach
The sample frame consists of young consumers from India’s metropolitan cities. The respondents were in the range of 18 to 35 years of age. These cities provide a high standard of living, more career options and better educational opportunities. Five separate sections of a standardised questionnaire were used, and a structural equation model was used to assess a total of 393 valid replies.

Findings
Green marketing impacts the behaviour that influences the desire of young customer to buy green real estate.

Research limitations/implications
As the focus of this study is primarily on major cities, future research may study similar behaviour in non-metropolitan cities. The study can also be conducted among consumers of other age groups.

Originality/value
The originality, to the best of the author’s knowledge, exists in examining how young consumers’ opinions about green marketing impact their intentions to purchase green houses and real estate in India. This study will be accessible to all parties involved in the housing and real estate industries.
Genealogy of the fair information practice principles
Authors: Stamenkov Gjoko 
Published: April 2023

Purpose
The purpose of this general review is to address the evolution and development of the Fair Information Practice Principles (FIPPs).

Design/methodology/approach
This study presents FIPPs from several establishments, compare them and map them to the General Data Protection Regulation (GDPR). Additionally, this study presents and discuss similarities and differences among FIPP sets.

Findings
Although the subject matter of the FIPP sets is very similar, there are differences: their scope differs significantly. The comparison among FIPP sets is presented, and it provides relevant information related to the connectedness between privacy principles.

Originality/value
This study considers the GDPR to be the pinnacle of the efforts to improve personal data protection; it became a role model for other countries to implement similar regulations.
Why do we twitch? Vicarious consumption in video-game livestreaming
Authors: Kohls Harper , Hiler Jacob L. , Cook Laurel Aynne 
Published: April 2023

Purpose
This study aims to examine vicarious consumption (VC) via the video-game streaming platform Twitch. The authors posit that watching someone play can offer the same enjoyment (measured through emotional experience, mood and joy) as playing.

Design/methodology/approach
A mixed-methods approach was used. A qualitative phase involving semistructured qualitative interviews, naturalistic inquiry and netnography generated testable hypotheses, which were tested using a two-condition, between-subjects field experiment.

Findings
This research advances the understanding of vicarious and experiential consumption by finding evidence that VC can produce the same levels of emotional experience, mood, attitude toward the product, joy, brand community loyalty and positive word of mouth. It also demonstrates the moderating effect of familiarity on mood change.

Research limitations/implications
This research demonstrates evidence that VC can offer outcomes similar to active consumption (AC). The authors advance research on VC in a new context (video-game livestreaming vs esports and other contexts) and from a new perspective (viewing motivations vs consumer-oriented outcomes). This research thus presents opportunities to explore these and other affective, behavior and cognitive outcomes in other contexts.

Practical implications
To reach Twitch users, marketers must understand how and why media consumers watch. This research provides insight into the community necessary to create effective advertising.

Originality/value
Building upon Sjöblom and Hamari, focusing on motivations for VC of esports and other related works, the authors expand the context to video-game livestreaming as a whole and examine affective, behavioral and cognitive outcomes compared with AC. Though VC has been researched and conceptualized theoretically, empirical testing is rare. This research offers empirical evidence that VC can offer the same levels of enjoyment as AC.
Auditor choice and audit fees through the lens of agency theory: evidence from Chinese family firms
Authors: Rahman Md Jahidur , Zhu Hongtao , Hossain Md Moazzem 
Published: April 2023

PurposeFrom an agency perspective, the authors investigate whether family ownership and control configurations are systematically associated with a firm's choice of auditor and audit fees. Agency theory is an economic theory that purposes the existence of a contract between two parties, principals and agents. Auditor choice and audit fees by family firms provide interesting insights given the unique nature of the agency problems faced by such firms.Design/methodology/approachThe authors employ Big-4 auditors (PWC, KPMG, E&Y and Deloitte) as a proxy for high quality auditor (Big N) for the auditor choice model. For the audit fee model, the dependent variable is the natural logarithm of audit fees (LnAF). The authors use two measures for family firm as explanatory variables: (1) a dummy variable (FAM_Control), which equals one if the firm is classified as a family firm and (2) FAM_Ownership, which is an indicator variable with a value of one if a firm has family members who hold CEO position, occupy board seats, or hold at least 10% of the firm's equity. Data of Chinese listed firms from 2011 to 2021 are used. The authors adopt the Heckman (1979) two-stage model to mitigate the potential endogeneity issue involved in the selection of Big-N auditors.FindingsThe findings suggest that compared with non-family firms, Chinese family firms have a less tendency to employ Big-4 auditors due to less severe agency problems between owners and managers. Additionally, Chinese family firms sustain higher audit fees than non-family firms. Similar to the prior literature, however, Chinese family firms audited by Big-4 auditors incur lower audit fees than family firms audited by non-Big-4 auditors in this study. In contrast to young-family firms, old-family firms are less likely to pick top-tier auditors and sustain lower audit fees. Consistent and robust results are found from endogeneity tests and sensitivity analyses.Originality/valueThe empirical evidence provides a unique insight, for accounting practitioners, policymakers, family owners and other capital market participants concerning the diverse effects of various family ownership and control features on selecting high-quality auditors and audit fees. This study advances the understanding, showing that a lower demand for audit quality occurs in Chinese family firms as they encounter less severe Type I agency problems. However, the more severe Type II agency problems in Chinese family firms sustain higher audit fees due to higher audit risk and greater audit effort.
The role of virtue ethics in enhancing reputation through combatting financial crimes
Authors: AlKhouri Ritab , Halteh Pashaar , Halteh Khaled , Tiwari Milind 
Published: April 2023

Purpose
This paper aims to outline how certain lessons from ethical systems can be relevant and applicable to tackling unethical behavior, including financial crime, within the finance profession.

Design/methodology/approach
This paper adopts a pragmatic perspective while acknowledging that there is a myriad of reasons managers act unethically, including the reality that many do so knowingly and deliberately. The matter is further complicated by human nature, given an individual’s behavior (ethical or unethical) is not easily discernable from their psychological, sociological, theological or cultural attributes.

Findings
Although such systems may not solve the problem of corrupt behavior, research suggests that industry professionals can learn to act in a more responsible and ethical manner. Given the wounded reputation of the financial sector, owing to their role in committing financial crimes such as money laundering, advances in ethical conduct would elevate both the effectiveness of the sector, as well as its reputation.

Originality/value
It is impractical to think we can completely resolve the problem of unethical behavior. Improvement, however, seems possible through promoting virtuous character traits and ethical behavior in individuals and organizations. Virtue ethics can play a significant role in combating financial crime and supporting anti-money laundering initiatives.
Unlocking supply chain product and process innovation through the development of supply chain learning capabilities under technological turbulence: Evidence from Egyptian SMEs
Authors: Abdelaziz Mahmoud Abdelaziz Ahmed , Wu Jiani , Yuan Changwei , Ghonim Mohamed Ahmed 
Published: April 2023

PurposeIn light of the current challenges in the business environment, firms, particularly those involved in supply chains, must foster innovation. In this context, the current study employs the theory of dynamic organizational capabilities (DOCs) to track supply chain learning capabilities (SCLCs) and independently uncover their relationship to innovation at both the product and process levels. Similarly, the study intends to investigate the influence of technological turbulence (TT) on these relationships as a moderating variable.Design/methodology/approachThe data were gathered using in-person interviews with 189 CEOs with some supply chain management proficiency from small and medium-sized enterprises (SMEs) in the industrial zones of eastern Egypt. The study used a survey approach to collect data, and the SEM-PLS technique was utilized to analyze the data.FindingsStudy findings revealed that SCLCs positively affect product and process innovation. In addition, TT positively moderates the relationship between SCLCs and product and process innovation, except for risk-taking capability. Further theoretical and practical implications are derived from the study findings.Originality/valueThis research adds to the knowledge of the dynamic capabilities theory (DCT), which affects how firms interact with their external environment. Studying learning capabilities are employed as essential competencies to counterbalance high levels of TT in the external environment in terms of innovative performance and vice versa if firms do not attempt to strengthen their dynamic learning capabilities in supply chains. In addition, this study contributes to the literature by studying learning capabilities from the external perspective, where SCLCs are being developed as a new variable to improve innovation.