bluuebunny/crossref_metadata_2025_split huggingface dataset:
Cbln1 Directs Axon Targeting by Corticospinal Neurons Specifically toward Thoraco-Lumbar Spinal Cord
Authors: Song Janet H.T. , Ruven Carolin , Patel Payal , Ding Frances , Macklis Jeffrey D. , Sahni Vibhu 
Published: February 2023

Corticospinal neurons (CSN) are centrally required for skilled voluntary movement, which necessitates that they establish precise subcerebral connectivity with the brainstem and spinal cord. However, molecular controls regulating specificity of this projection targeting remain largely unknown. We previously identified that developing CSN subpopulations exhibit striking axon targeting specificity in the spinal white matter. These CSN subpopulations with segmentally distinct spinal projections are also molecularly distinct; a subset of differentially expressed genes between these distinct CSN subpopulations regulate differential axon projection targeting. Rostrolateral CSN extend axons exclusively to bulbar-cervical segments (CSNBC-lat), while caudomedial CSN (CSNmedial) are more heterogeneous, with distinct, intermingled subpopulations extending axons to either bulbar-cervical or thoraco-lumbar segments. Here, we report, in male and female mice, thatCerebellin 1(Cbln1) is expressed specifically by CSN in medial, but not lateral, sensorimotor cortex.Cbln1shows highly dynamic temporal expression, withCbln1levels in CSN highest during the period of peak axon extension toward thoraco-lumbar segments. Using gain-of-function experiments, we identify that Cbln1 is sufficient to direct thoraco-lumbar axon extension by CSN. Misexpression of Cbln1 in CSNBC-lateither byin uteroelectroporation, or by postmitotic AAV-mediated gene delivery, redirects these axons past their normal bulbar-cervical targets toward thoracic segments. Further, Cbln1 overexpression in postmitotic CSNBC-latincreases the number of CSNmedialaxons that extend past cervical segments into the thoracic cord. Collectively, these results identify that Cbln1 functions as a potent molecular control over thoraco-lumbar CSN axon extension, part of an integrated network of controls over segmentally-specific CSN axon projection targeting.SIGNIFICANCE STATEMENTCorticospinal neurons (CSN) exhibit remarkable diversity and precision of axonal projections to targets in the brainstem and distinct spinal segments; the molecular basis for this targeting diversity is largely unknown. CSN subpopulations projecting to distinct targets are also molecularly distinguishable. Distinct subpopulations degenerate in specific motor neuron diseases, further suggesting that intrinsic molecular differences might underlie differential vulnerability to disease. Here, we identify a novel molecular control, Cbln1, expressed by CSN extending axons to thoraco-lumbar spinal segments. Cbln1 is sufficient, but not required, for CSN axon extension toward distal spinal segments, andCbln1expression is controlled by recently identified, CSN-intrinsic regulators of axon extension. Our results identify that Cbln1, together with other regulators, coordinates segmentally precise CSN axon targeting.
The role of parity in the relationship between endometriosis and pregnancy outcomes: a systematic review and meta-analysis
Authors: Sri Ranjan Yorain , Ziauddeen Nida , Stuart Beth , Alwan Nisreen A , Cheong Ying 
Published: February 2023

Graphical Abstract

Abstract
Endometriosis is a chronic and debilitating condition which can affect the entire reproductive life course of women with a potentially detrimental effect on pregnancy. Pregnancy (and increasing parity) can affect endometriosis by modulating disease severity and suppressing symptoms. Multiparous women could be less likely to suffer from endometriosis-related pregnancy complications than primiparous women. We aimed to systematically review the evidence examining the role of parity in the relationship between pregnancy outcomes and endometriosis. A systematic search of MEDLINE, EMBASE, CINAHL, Web of Science, and Cochrane Library was performed from inception to May 2022. We searched for experimental and observational studies. Grading of Recommendations, Assessment, Development, and Evaluation was used to assess the quality of evidence with the risk of bias in non-randomised studies of interventions tool incorporated. Eleven studies were included in the meta-analysis. Primiparous women with endometriosis had almost double the risk of hypertensive disorders of pregnancy (OR: 1.99, 95% CI: 1.50–2.63, P < 0.001) compared to multiparous women with endometriosis. Primiparous women with endometriosis were at significantly increased risk of preterm delivery, caesarean delivery, and placenta praevia compared to primiparous women without endometriosis. There were no significant differences in outcomes when multiparous women with endometriosis were compared to multiparous women without endometriosis. There is limited evidence to suggest that primiparous women with endometriosis may be at higher risk of adverse pregnancy outcomes compared to multiparous women. The modulatory role of parity in the pathophysiology of endometriosis and its impact on pregnancy outcomes should be investigated.

Lay summary
Endometriosis can adversely affect pregnancy and cause complications that can affect both mother and baby. The severity and symptoms of endometriosis are lessened in pregnancy and with increasing births. Women who have previously given birth could experience fewer pregnancy complications than women giving birth for the first time. We reviewed the literature to compare pregnancy outcomes in women with endometriosis by whether they had given birth before or not. Our review included 11 studies. More women with endometriosis giving birth for the first time had blood pressure disorders in pregnancy than women with endometriosis who had given birth before. First-time mothers with endometriosis tended to have a baby born early, caesarean delivery, and an abnormally located placenta compared to those without endometriosis. This study supports the theory that women with endometriosis in their first pregnancy are at higher risk of complications and may benefit from additional monitoring.
The CASR antagonist NPS2143 induces loss of acrosomal integrity and proteolysis of SPACA1 in boar spermatozoa
Authors: Macías-García Beatriz , González-Fernández Lauro 
Published: February 2023

In brief
The mechanism by which p32 protein increases during capacitation in boar spermatozoa is unknown. This manuscript shows a new mechanism of induction of p32 in boar spermatozoa: the proteolysis of the phosphorylated and glycosylated form of SPACA1.

Abstract
Protein tyrosine phosphorylation (PY) induction is associated with sperm capacitation. We previously showed that calcium-sensing receptor (CASR) inhibition by NPS2143 induces the 32 kDa tyrosine-phosphorylated protein (p32) in boar spermatozoa. We showed that NPS2143 induced an increase in p32 and loss of acrosomal integrity in live and dead spermatozoa in capacitating conditions (Tyrode's complete medium); the p32 rise occurred in dead spermatozoa, as shown by flow cytometry sorting. EGTA addition blunted the increase in p32, the loss of acrosomal integrity, and the increase in dead spermatozoa induced by NPS2143, indicating that the effects of NPS2143 are calcium-dependent. Mass spectrometry was used to identify which tyrosine-phosphorylated proteins were induced by NPS2143, but only serine/threonine-phosphorylated proteins were found; among these, SPACA1 was identified with different molecular weights (18, 32, and 35–45 kDa). We confirmed tyrosine phosphorylation of SPACA1 at 32 and 35–45 kDa by immunoprecipitation and co-localization of PY and SPACA1 in the presence of NPS2143 by immunofluorescence. The molecular weight of SPACA1 (35–45 kDa) decreased after treatment with peptide-N-glycosidase F, indicating that this protein is N-glycosylated. The soybean trypsin inhibitor (STI), a serine protease inhibitor, suppressed the appearance of p32 and SPACA1 (30 and 32 kDa) induced by NPS2143. Also, 8-Br-cAMP and A23187 treatments induced an increase in p32 and SPACA1 (30–32 kDa) and a parallel induction of the acrosome reaction. These findings suggest that CASR inhibition induces loss of acrosomal integrity and proteolysis of the glycosylated and phosphorylated SPACA1 (35–45 kDa) resulting in a SPACA1 rise at 32 kDa (p32).
Changes of Colorectal Cancer Diagnostics and Hospitalizations during First Wave of COVID-19 Pandemic in Lithuania
Authors: Jurkevičiūtė Dignė , Mauravičiūtė Sandra , Dulskas Audrius , Kildušienė Inga , Stratilatovas Eugenijus , Jarmalaitė Sonata 
Published: February 2023

Purpose: Our aim was to see the possible effect of the first COVID pandemic wave in Lithuania on colorectal cancer (CRC) preventive, diagnostic and treatment procedures.Methods: A retrospective analysis was performed using the database of the National Cancer Institute, Lithuania. We have divided patients into two groups: group 1 – patients treated during the nonpandemic period (2019 January 1 to 2019 July 31) and group 2 – the pandemic period (2020 January 1 to 2020 July 31). We analyzed numbers of screening, therapeutic colonoscopies performed, and treated patients for CRC during two periods.Results: In general, 1318 lower gastrointestinal endoscopic procedures were performed in the first group and 862 procedures in the second group, which was 34.6% less compared to the first group. The first group included 672 (51%) colonoscopies, 172 (13%) day surgeries and 474 (36%) CRC screening programmes. In group 2, 456 (34.6%) less patients underwent CRC diagnostics and treatment: 141 (21%) less colonoscopies, 93 (54%) less day surgeries, 222 (47%) less CRC screening programmes, and 26 (13%) less patients were hospitalized for surgical treatment (196 vs 170).Conclusion: Our study reveals worrying changes in the timely access to diagnostic procedures during the COVID-19 pandemic that possibly provoked rise in cases with the advanced stage CRC. However, despite numerical difference between groups existed, the difference between groups do not reach statistical significant level.
ordem política como tarefa
Authors: Lima Deyvison , Arruda José Maria 
Published: February 2023

O artigo trata da obra de Carl Schmitt e sua infl uência sobre o ordoliberalismo alemão. Tal chave de leitura permite compreender as causas da crise das instituições modernas e o liberalismo autoritário, inclusive como matriz da governamentalidade neoliberal. A hipótese da pesquisa indica que a obra schmittiana concede um acesso privilegiado para analisar as tendências autoritárias como a expressão do esgotamento da política moderna, a rejeição da democracia e as relações entre liberalismo e teologia política. Afinal, se a economia pressupõe uma ordem, esta seria uma decisão política, não um produto econômico. Na primeira parte, apresenta o conceito do político como uma tentativa de realização da Ordnungspolitik em contraposição à ausência de forma política; logo, de autoridade. Na segunda parte, avalia a proposta do Estado Total como o resultado das transformações do liberalismo e da intensificação do político na “era da técnica”. A conclusão interpreta a pulsão de ordem do ordoliberalismo na década de 1930 como a tentativa de fornecer um quadro institucional para produzir e assegurar a concorrência por meio de um Estado forte, além de iluminar algumas ambiguidades da relação entre mercado e Estado até sua deriva neoliberal.
Para além da paranoia epistêmica
Authors: Pinho Thiago 
Published: February 2023

A Ontologia Orientada ao Objeto (OOO) é uma linha filosófica contemporânea muito difundida no universo acadêmico, com vários debates acalorados ao longo do globo, mas ainda bastante tímida quando o assunto é o campo da Teoria Social. O objetivo desse ensaio é compreender esse ponto de cruzamento, muitas vezes até de tensão, tomando como ponto de partida as ideias de Graham Harman e suas implicações epistêmico-ontológicas. Como resultado, existe nesse nosso horizonte investigativo uma nova atmosfera teórica, uma nova forma de mergulhar no campo das ciências humanas e sociais, o que vai ser chamado aqui de Teoria Social Orientada ao Objeto (T. S. O. O). Quais os desdobramentos de uma T. S. O. O? Quais são suas vantagens diante de outras tradições já existentes? Essa e outras perguntas vão ser respondidas ao longo destas páginas, pelo menos, enquanto um breve aperitivo.
Comparación del infrarrojo-cercano fecal con métodos convencionales para estimar consumo y digestibilidad en ovinos
Authors: Parra-Forero Diana , Mayorga Mogollón Olga , Ariza-Nieto Claudia 
Published: February 2023

Introducción. El consumo y la digestibilidad son parámetros que definen la calidad de un forraje, sin embargo, son difíciles y costosos de estimar. La tecnología espectroscopía de infrarrojo cercano aplicada a las heces (NIRSf) es una alternativa a los métodos convencionales de referencia para estimar el consumo voluntario (CVMS) y digestibilidad de materia seca (DMS) en ovinos. Objetivo. Comparar la tecnología NIRSf con métodos convencionales para la estimación de CVMS y DMS en ovinos en confinamiento. Materiales y métodos. Se realizaron seis bioensayos en el centro de investigación Tibaitata, Cundinamarca, Colombia, durante los años 2019 y 2021 con cinco ovinos (PV 58,28±11 kg) para estimar CVMS y DMS por tres métodos: gravimetría, marcadores y NIRSf. Los animales fueron alimentados con seis regímenes alimenticios contrastantes en su valor nutricional. Se colectaron muestras de forrajes y heces, las cuales se secaron y molieron para su posterior análisis químico y espectral. Resultados. La estimación de CVMS y DMS fue diferente (p<0,001) en los seis regímenes alimenticios evaluados, donde el CVMSPM varió de 37,54 a 82,58 g/kg PV0,75 y la DMS varió de 36,32 a 58,81 %. En la comparación de la estimación del CVMS y DMS por el método referente (gravimétrico) con los métodos de marcadores y NIRSf, se observó que el método NIRSf presentó mejor ajuste comparado con el de marcadores, al presentar menor valor de la raíz del cuadrado medio del error (-1,53 y -1,75, respectivamente), menor error medio absoluto (-3,01 y -0,5, respectivamente) y mayor coeficiente de determinación (+0,09 y +0,28, respectivamente). Conclusión. La estimación del CVMS y la DMS por medio de ecuaciones NIRSf presentó mejor ajuste comparado con la metodología de marcadores; sin embargo, es necesario mejorar la precisión de las calibraciones con muestras de heces de animales bajo diferentes contextos productivos.
Das Bild Ungarns in Österreich
Authors: Kokai Karoly 
Published: February 2023

AbstractBy comparing the colored etching Aigentliche Vorstell- und Beschreibung der fürnehmsten in Europa befindlicher Land-Völcker, Augsburg around 1700, and the journal Acta Comparationis Litterarum Universarum, Kolozsvár around 1880, imagology, a research area of comparative cultural and literary studies, can be discussed in a comparative manner. Based on the findings of this comparison, the article seeks answers to questions about the scientific location and the task of Hungarian studies outside of Hungary.
Differences in explosive strength values for students of the faculty of physical education and sports (male) according to body mass index levels
Authors: Mocanu George D. , Murariu Gabriel , Potop Vladimir 
Published: February 2023

Background and Study Aim. Explosive strength/muscular strength is demanded at the level of different body segments and regions in most sports. The purpose of the research: is to identify the differences in the manifestation of explosive force between groups of underweight, normal weight and overweight university students; to determine the associations between the anthropometric parameters and the value of the results in the applied tests.
Material and Methods. The investigated group consists of 147 students (men) of the Faculty of Physical Education and Sport, divided into 3 distinct groups for the analysis of the results: underweight (age=20.40±1.18, BMI=17.81±0.93), normal weight (age=20.504±1.671, BMI =22.24±1.67) and overweight (age=22.44±2.24, BMI=28.01±2.74). 7 tests were used to evaluate the explosive strength of the lower body (Vertical Jump Test, Standing Long Jump Test, 3-Hop Test, The multiple 5 bounds test, 30s Lateral double leg hop test, 30s Continuous vertical jumps, Speed Test 10m). A number of 6 tests were used to evaluate the explosive strength of the upper body (Overhand ball throw, Shot put, Overhead Medicine Ball Throw-forward, Overhead Medicine Ball Throw-backward, Medicine ball chest throw, 30s Plyometric Push-Ups).
Results. Univariate test results indicate F values associated with significant thresholds at the lower body level (P<0.05) for tests based on horizontal jumps (Standing Long Jump, 3-Hop Test and The multiple 5 bounds test), where underweight and normal weight have the better average scores. At the level of the upper body, the situation is changed (for Shot put and medicine balls throws), where the overweight have the best average values, followed by the normal weight, and the worst results are found for the underweight group (P<0.05). Only for the Overhand ball throw and 30s Plyometric Push-Ups, the superiority of the overweight is not statistically confirmed (P>0.05). Correlation calculation (Pearson values) indicates positive associations between body height and vertical and horizontal jumps (except for those repeated for 30s), but negative associations of BMI and body mass with jump-based tests. However, BMI and body mass are moderately, positively and significantly correlated with throw-based tests (P<0.05).
Conclusions. We can state that the classification of students in different BMI categories generates differences in explosive strength values between the 3 studied groups. The comparisons indicate the superiority of underweight and normal weights over overweight in all lower body explosive strength tests. The comparisons indicate also the significant superiority of overweight in the medicine ball and shot put tests over normal and underweight. The obtained results cannot be generalized, due to the small size of the underweight and overweight samples. Further investigations on larger groups of university students being necessary.
Etiology and clinical features of non-O1/non-O139 Vibrio cholerae infection in an inland city in China
Authors: Xiang Yu-Han , Hu Qin-Qin , Liu Yan , Sheng Rui , Wang Jie , Li Wen-Jing , Shi Jian , Li Xue , Lu Shu-Hua 
Published: February 2023

Non-O1/non-O139 Vibrio cholerae (NOVC) causes various illnesses ranging in severity from mild to life-threatening but were ignored previously. Knowledge of the NOVC infection, particularly bacteremia, is limited because of its rarity. Here we first retrospectively reported the demographic, clinical, and therapy characteristics of patients with NOVC infection. Isolated NOVC stains were identified by a series of biochemical, mass spectrometry (MS), and serum agglutination tests. The results of 11 patients with NOVC infection (including 8 with bacteremia) with a median age of 68 years were included in this report. Most isolated NOVC strains had antibiotic susceptibility. Patients with NOVC-positive were distributed in various departments, most occurring in gastroenterology (6 cases). Hepatic disease was the most common comorbid disease, followed by diabetes (3 cases) and biliary tract disease (3 cases). Two cases were previously healthy. The most common symptom at presentation was fever. All patients presented with abnormal changes in hematology and inflammatory parameters. Cephalosporins were the most frequently used antibiotics. Ten patients had a favorable outcome after treatment; one died from complicated underlying diseases. In summary, we recommend the timely identification of NOVC strains using MALDI-TOF-MS. The suspicion of NOVC bacteremia cannot be ruled out regardless of the host’s immune status. An alternative therapeutic regimen for this infection may be β-lactam antibiotics or combined with β-lactamase inhibitors. Regardless, the specific therapeutic regimen should be based on the antibiogram data.
Amniotic fluid is a marker of the condition of the fetus. Is it so?
Authors: Bezhenar Vitaly F. , Ivanova Lidiia A. , Ivanov Dmitry O. , Krasnogorskaya Olga L. 
Published: February 2023

BACKGROUND: In the presence of meconium in the amniotic fluid, childbirth is usually classified as a high-risk group, which leads to an expansion of indications for operative delivery. Also in the literature there are indications of an increase in the frequency of the birth of a child in a state of asphyxia, with premature rupture of amniotic fluid.
AIM: The aim of this study is to determine the significance of the influence of meconium color of amniotic fluid and the duration of the anhydrous period on the birth of a child in a state of asphyxia.
MATERIALS AND METHODS: A retrospective study was conducted between the two groups in 12,342 women delivered at the Perinatal Center of the Pediatric University.
RESULTS: Meconium coloration of amniotic fluid was 2 times more common in the presence of hypoxia, and 5 times more common in the presence of severe fetal hypoxia during full-term pregnancy, while in premature pregnancy, the child was born in a state of asphyxia, regardless of the color of the amniotic fluid. Premature rupture of amniotic fluid occurred at the birth of a full-term baby in a state of asphyxia statistically significantly more often, although the duration of the anhydrous period did not have statistically significant differences in the groups.
CONCLUSIONS: Premature rupture of amniotic fluid at the birth of a premature baby had no differences in the groups with and without asphyxia, however, the duration of the anhydrous period was statistically significantly longer in patients who gave birth to children in a state of asphyxia. With an anhydrous interval of 26 hours or more, the risk of having a child in a state of moderate and severe asphyxia increased. The risk of ascending infection of the placenta of the third stage during preterm labor increased with an anhydrous interval of more than 53 minutes, which confirms the feasibility of prescribing antibiotic therapy for premature rupture of amniotic fluid during preterm pregnancy at the time of diagnosis of this condition, regardless of the planned obstetric tactics.
COVID-19'A BAĞLI YAŞANAN KORKUNUN ÜNİVERSİTE ÖĞRENCİLERİNDE DUYGUSAL İŞTAH VE YEME DAVRANIŞLARINA ETKİSİNİN DEĞERLENDİRİLMESİ
Authors: TURANLI Fidan Nur , ARSLAN Sedat 
Published: February 2023

Giriş: Bu calışmanın amacı, COVID-19 pandemisi sürecinde örgün eğitimin sekteye uğraması, alışılagelmiş ya da beklenen üniversite hayatı sürecinin sekteye uğramasıyla birlikte gelişen olumsuz duyguların ve yaşanan korkunun duygusal iştah ve yeme davranışlarına etkisini değerlendirmektir.
Yöntem: Araştırma kesitsel ve tanımlayıcı tipte bir çalışmasıdır. İstanbul Okan Üniversitesi öğrencilerinden 385 öğrenci araştırmaya dâhil edilmiştir. Çalışmada katılımcılara; Covid-19 Korkusu Ölçeği, Duygusal İştah Anketi, Hollanda Yeme Davranışı ölçekleri uygulanmış olup 24 saatlik Besin Tüketim Kaydı’da alınmıştır.
Bulgular: Araştırmada COVID-19’un duygusal yeme ve dışsal üzerinde etkisinin olmadığı (p>0.05), kısıtlayıcı yeme üzerinde %1.6 arttırıcı etkisi olduğu (p