bluuebunny/crossref_metadata_2025_split huggingface dataset:
Organic enrichment negatively impacts meiobenthic copepods on Argentinean coasts
Authors: Sciberras Michel , MENECHELLA AGUSTIN G. , PÉREZ-MÉNDEZ NÉSTOR , CAZZANIGA NÉSTOR J. , MARRERO HUGO J. 
Published: August 2023

Abstract
Organic enrichment is a major threat to coastal environments, yet its impact on meiobenthic animals remains poorly understood. In marine sediments, copepods belong to the dominant taxa and they are known to regulate organic matter degradation and nutrient cycling. Here we performed a large-scale observational study to assess the impact of an increase in organic matter on meiobenthic copepod. We established a relation between the content of organic matter that reaches the coasts through eight rivers and the richness and diversity of meiobenthic copepods. In each site, three sampling points were considered (river-mouth, 200 m north, and 200 m south) and for each of these we estimated taxa diversity and taxa richness, organic matter concentration, and environmental parameters. We found that organic enrichment was negatively correlated with both taxa diversity and taxa richness, and this relation was stronger at river mouths. We also found a synergic interaction between salinity and organic matter content; instead, temperature and pH seemed not to be structuring the copepod community. This study provides evidence of the potential negative impact of coastal organic enrichment on the copepod diversity.
The Classification of the Bladder Cancer based on Vision Transformers (ViT)
Authors: Khedr Ola S. , Wahed Mohamed E. , Al-Attar Al-Sayed R. , Abdel-Rehim E. A. 
Published: August 2023

Abstract
Bladder cancer is a prevalent malignancy with diverse subtypes, including invasive, non-invasive, and normal tissue. Accurate classification of these subtypes is crucial for personalized treatment and prognosis. In this paper, we present a comprehensive study on the classification of bladder cancer into three classes using various deep-learning models. We utilized a dataset containing histopathological images of bladder tissue samples, split into a training set (70%), a validation set (15%), and a test set (15%). Four different deep-learning architectures were evaluated for their performance in classifying bladder cancer, EfficientNetB2, InceptionResNetV2, InceptionV3, and ResNet50V2. Additionally, we explored the potential of Vision Transformers with two different configurations, ViT_B32 and ViT_B16, for this classification task. Our experimental results revealed significant variations in the models' accuracies for classifying bladder cancer. The highest accuracy was achieved using the InceptionResNetV2 model, with an impressive accuracy of 98.73%. Vision Transformers also showed promising results, with ViT_B32 achieving an accuracy of 99.49%, and ViT_B16 achieving an accuracy of 99.23%. EfficientNetB2 and ResNet50V2 also exhibited competitive performances, achieving accuracies of 95.43% and 93%, respectively. In conclusion, our study demonstrates that deep learning models, particularly Vision Transformers (ViT_B32 and ViT_B16), can effectively classify bladder cancer into its three classes with high accuracy. These findings have potential implications for aiding clinical decision-making and improving patient outcomes in the field of oncology.
Effect of intracerebroventricular (ICV) injection of adrenomedullin and its interaction with NPY and CCK pathways on food intake regulation in neonatal layer-type chicks
Authors: Soleymani Maryam , Alimohammadi Samad , Hassanpour Shahin 
Published: August 2023

Abstract
Background
Adrenomedullin has various physiological roles including appetite regulation. The objective of present study was to determine the effects of intracerebroventricular injection of adrenomedullin and its interaction with NPY and CCK receptors on food intake regulation in layer-type chicks. In experiment 1, chickens received ICV injection of saline and adrenomedullin (1, 2 and 3 nmol). In experiment 2, birds injected with saline, B5063 (NPY1 receptor antagonist, 1.25 µg), adrenomedullin (3 nmol) and co-injection of B5063 + adrenomedullin. Experiments 3–5 were similar to experiment 2 and only SF22 (NPY2 receptor antagonist, 1.25 µg), SML0891 (NPY5 receptor antagonist, 1.25 µg) and CCK4 (1 nmol) were injected instead of B5063. In experiment 6, ICV injection of saline and CCK8s (0.125, 0.25 and 0.5 nmol) were done. In experiment 7, chickens injected with saline, CCK8s (0.125 nmol), adrenomedullin (3 nmol) and co-injection of CCK8s + adrenomedullin. After the ICV injection, birds were returned to their individual cages immediately and cumulative food intake was measured at 30, 60 and 120 min after injection.
Results
According to the results, adrenomedullin (2 and 3 nmol) decreased food intake compared to control group (P < 0.05). Co-injection of the adrenomedullin + B5063 amplified hypophagic effect of adrenomedullin (P < 0.05). The ICV injection of the CCK8s (0.25 and 0.5 nmol) reduced food intake (P < 0.05). Co-injection of the CCK8s + adrenomedullin significantly potentiated adrenomedullin-induced hypophagia (P < 0.05). Nevertheless, administration of the SF22, SML0891 and CCK4 had no effect on the anorexigenic response evoked by adrenomedullin in chickens (P > 0.05).
Conclusion
These results suggested that the hypophagic effect of the adrenomedullin is mediated by NPY1 and CCK8s receptors. However, our novel results should form the basis for future experiments.
A method of constructing highly durable conductive materials by growing metal particles inside and outside fibers through solvation and multivalent bonding forces
Authors: Wen Yan , Du Peibo , Sun Chao , Zhan Xiongwei , Cai Zaisheng , Ge Fengyan 
Published: August 2023

Abstract
Metallized textiles have shown promising applications in the fields of electrical conductivity, Joule heating and electromagnetic shielding. Poor durabilities, especially washability, which caused by the significant mechanical mismatch between rigid metal parts and textiles have hindered commercialization process of these functional textiles. This work constructed ultra-durable conductive cotton fabrics by growing copper nanoparticles with amorphous region-controlled swelling and multivalent bonding forces to complex the metal particles. The enlarged fiber amorphous zone and phen-amine molecules are used as templates to provide further possibilities for the internal and external enrichment growth of copper nanoparticles, providing good conductivity and high durability of the processed cotton fabric. The constructed fabric exhibits excellent electrical conductivity (6.09±0.36×10-3 Ω/sq), electrothermal conversion (60 s, 1 V, ~140 °C) and electromagnetic shielding efficiency (65.32 dB). Notably, the electrical conductivity of the fabric remains essentially unchanged (Rs/R0=1.106) after 100 standard washing tests. This is attributed to the increase in metal particle loading and the enhancement of metal-fiber bonding fastness. Therefore, this work might provide a novel insight for constructing ultra-washable conductive clothing textiles with heating and EMI shielding performance.
A Novel Framework to Classify Cancer through a Consecutive Utilization of Hybrid Feature Selection and Deep Learning
Authors: Mahto Rajul , Ahmed Saboor Uddin , Rahman Rizwan ur , Aziz Rabia Musheer , Roy Priyanka , Mallik Saurav , Li Aimin , Shah Mohd Asif 
Published: August 2023

Abstract
Cancer prediction in the early stage is a topic of major interest in medicine since it allows accurate and efficient actions for successful medical treatments of cancer. Mostly cancer datasets contain various gene expression levels as features with less samples, so firstly there is a need to eliminate similar features to permit faster convergence rate of prediction algorithms. These features (genes) enable us to identify cancer disease, choose the best prescription to prevent cancer and discover deviations amid different techniques. To resolve this problem, we proposed a hybrid novel technique CSSMO-based gene selection for cancer prediction. First, we made alteration of the fitness of Spider Monkey Optimization (SMO) with Cuckoo search (CS) algorithm viz., CSSMO for feature selection, which helps to combine the benefit of both metaheuristic algorithms to discover a subset of genes which helps to predict a cancer disease in early stage. Further, to enhance the accuracy of the CSSMO algorithm, we choose a cleaning process, minimum redundancy maximum relevance (mRMR) to lessen the gene expression of cancer datasets. Next, these subsets of genes are classified using Deep Learning (DL) to identify different groups or classes related to a particular cancer disease. Six different datasets have been utilized to analyze the performance of the proposed approach in terms of cancer sample classification and prediction with Recall, Precision, F1-Score, and confusion matrix. The proposed gene selection method with DL achieves much better prediction accuracy than other existing Deep Learning (DL) and Machine learning models with large gene expression dataset.
Impact of chronic infections in pregnancy: Association between maternal seropositivity for Toxoplasma gondii and fetal growth restriction
Authors: Martinez Victor Otero , Santos Nathália Ribeiro dos , Bah Homègnon Antonin Ferréol , Junior Erival Amorim Gomes , Costa Daisy Oliveira , Menezes-Filho José Antônio 
Published: August 2023

Abstract
Insults caused by acute infections during the gestational period on fetal development are known; however, new evidence suggests that chronic infectious diseases can also impact the maternal immune status and lead to negative consequences for the neonate. This study investigated the association between the prevalence of specific antibodies in pregnant women and alterations in fetal development at birth. A follow-up study evaluated women during the gestational period and their respective newborns at delivery time. The pregnant women were tested for the presence of antibodies to infectious agents: Toxoplasma gondii (T. gondii), cytomegalovirus (CMV), syphilis, human immunodeficiency virus (HIV), Hepatitis B, and C. Semi-structured questionnaires were administered to the pregnant women at the time of recruitment after obtaining informed consent. Detailed information about the newborns was extracted from medical records. The overall seroprevalence of T. gondii infection was 56.2%, and the general prevalence of CMV IgG antibodies was 96.3%. Non-primiparous pregnant women from socio-economic classes D-E and C2 had higher chances of testing positive for specific T. gondii IgG antibodies. Newborns classified as small for gestational age represented 12.9% of the total. Those born to mothers seropositive for anti-T. gondii IgG antibodies were 9.4 times more likely to be born small for gestational age (p = 0.035). The results suggest that chronic T. gondii infection may contribute to higher rates of newborns with growth restriction. These findings add to a growing body of evidence regarding the impact of chronic infectious diseases on intrauterine fetal development.
Study of corrosion behavior of 321 stainless steel in NaCl solution
Authors: Li Yintao , Wang Yutuo , Zhang Zhengquan , Zhou Yuanlin , Wang Shanqiang 
Published: August 2023

Abstract
321 stainless steel has high room temperature strength and oxidation resistance. It is investigated that the corrosion resistance of 321 stainless steel in hydrochloric acid solutions containing different concentrations of NaCl in the research. The alloy is mainly composed of austenitic stainless steel. The results of electrochemical tests were analyzed, and it was confirmed that NaCl could promote the corrosion of 321 stainless steel, but the corrosion would be suppressed to a certain extent when the NaCl concentration reached 0.10 mol/L. The research results can provide a reference for the corrosion resistance improvement research of 321 stainless steel.
Effects of flowering mode and pollinator sharing on reproductive success in natural hybrid of two Epimedium (Berberidaceae) species
Authors: Chen Lan-ying , Quan Qiumei , Li Yunxiang 
Published: August 2023

Abstract
Natural hybridization mediated by pollen flow has been considered as an important factor to drive speciation in floral plants. The diversity of Epimedium species is centered in southwestern China, yet the potential role of interspecific pollination in natural hybridization has not been empirically examined. The flowering period and floral visitors, and conducted a series of pollination experiments to examine breeding systems and hybrid compatibility within three Epimedium which are commonly sympatric in the Jinchengshan National Foreast Park, Nanchong of Sichuan Province. Epimedium pubescens and Epimedium wushanense are morphologically clearly distinct and easily recognied by flower morphology, the putative hybrids (not been named) are morphologically between them. We found that E. pubescens and E. wushanense had 15 days of overlapping anthesis during consecutive three years of observation. Both species in research region are self-incompatible and need pollinators service for successful reproduction. Their pollinators are different in quantity and species, but Lasioglossum spp. (Hymenoptera: Halictidae) visited all of them. No breeding barrier between any of the three species were detected by artificial pollination, and the hybrids seeds were fertile. The overlapping time of flowering, common floral visitors and compatible of hybrid may be important factors to make interspecific pollination and speciation successfully for natural hybrids species of E. pubescens and E. wushanense. The role of potential interspecific pollination is essential to hybrids speciation and the evolution of Epimedium species.
Bone marrow stromal antigen 2 is associated with immune microenvironment of colorectal cancer
Authors: Fu Yuewu , Fu Tiannan , Zhou Youtao 
Published: August 2023

Abstract
Aims
This study aimed to investigate the involvement of bone marrow stromal antigen 2 (BST2) in the immune microenvironment of colorectal cancer (CRC).
Methods
BST2 expression profiles, clinical information, and chemosensitivity data of CRC patients were downloaded from TCGA, GEO, and GDSC databases. The relationship between BST2 and immune cell infiltration was evaluated using CIBERSORT and TIMER. Immune-related coexpressed genes of BST2 were identified by GeneMANIA and Immport. A prognostic model containing BST2 and immune-related genes was constructed and tested by ROC curves. Kaplan-Meier plot was used for survival analysis. BST2 promoter methylation and the correlation of BST2 expression with TMB and MSI were assessed using UALCAN and SangerBox.
Results
BST2 mRNA levels were significantly increased in high-stage CRC tumors compared with those in low-stage tumors and correlated with poor survival of patients. The fractions of M1 macrophages, CD8+ T cells, and gamma delta T cells were markedly increased in patients with high BST2 expression versus those with low BST2 expression. IFITM1, ISG15, MX1, and OAS1 were identified as immune-related coexpressed genes of BST2 in CRC and performed well in predicting the overall survival of patients. Furthermore, BST2 expression was associated with DNA methylation and positively correlated with TMB and MSI in CRC. BST2 expression also positively correlated with IC50 values of chemotherapeutic agents in patients with MSI-L/MSS CRC.
Conclusion
BST2 upregulation is associated with the immune microenvironment of CRC and poor prognosis of patients. Patients with high expression of BST2 or MSI-H CRC may respond well to immunotherapy.
Warfarin tautomers in solution: a structural, computational and thermodynamic study
Authors: Osborne Daniel A. , Danielyan Edward , Hoang Khoi , Valente Edward 
Published: August 2023

Abstract
Variable temperature NMR spectroscopic measurements on (S)-warfarin [open-form: 3-(1’-phenyl-3’-oxobut-1’-yl)-4-hydroxycoumarin] in CDCl3, CD3OD and d6-DMSO generally showed tautomeric compositions in the order trans (2S,4S) coumarin hemiketal > cis (2R,4S) coumarin hemiketal > open (S) coumarin enol in slow dynamic equilibrium over temperature ranges rising modestly from ambient. A computational (DFT - M06-2X) examination of the lower energy tautomers including coumarin and chromone open and cyclic forms (gas phase, chloroform or DMSO fields) was consistent with the general solution compositions. The crystal and molecular structures for model compounds of the major solution tautomers are reported: (2S,4S)-warfarin methyl ketal [orthorhombic, P212121], (2R,4S)-warfarin methyl ketal [orthorhombic, P212121], (rac)-warfarin-4-methyl ether [monoclinic, P21/n], and the open chromone (S)-warfarin-2-methyl ether [monoclinic, P21, Z = 8]. A combination of direct integration and line-fitting methods were used to determine solution (S)-warfarin tautomer compositions. As temperatures were increased, the concentrations of the open coumarin form increased at the expense of the cyclic hemiketals. Equilibrium constants were used to determine the standard free-energy differences for the two open-cyclic equilibria (trans hemiketal open, open cis hemiketal, respectively) in three solvents: CDCl3 [+ 3.7(4), -2.8(6) kJ/mol], CD3OD [+ 7.6(16), -4.7(9) k/mol], d6-DMSO [+ 3.5(7), -1.1(2) kJ/mol]. Standard enthalpy and entropy differences were also determined from van’t Hoff analysis. Rates of the respective reactions were estimated from line-widths for the cyclic hemiketals and solution equilibrium compositions for each species. Eyring analysis gave ΔG‡, ΔH‡, and ΔS‡, respectively, for the forward and reverse reactions of coumarin trans hemiketal open-form and for the open-form cis hemiketal. Negative entropic contributions to the observed transition state energies were consistent with solvent or solute ordering in the prototropic reactions. Open-form NMR signals were broader than could be accounted for by the open-cyclic equilibria alone, increasingly so in polar and protic solvents and with rising temperatures. While a conformational equilibrium may operate, an increasingly faster intermediate dynamic equilibrium between open coumarin-chromone tautomers may be a more likely explanation.
Comparative analysis of transposable elements reveals the diversity of transposable elements in decapoda and their effects on genomic evolution
Authors: Xu Yuanfeng , Tang Yongkai , Feng Wenrong , Yang Yanan , Cui Zhaoxia 
Published: August 2023

Abstract
Transposable elements (TEs) are mobile genetic elements that exist in the host genome and exert considerable influence on the evolution of the host genome. Since crustaceans, including decapoda, are considered ideal models for studying the relationship between adaptive evolution and TEs, TEs were identified and classified in the genomes of eight decapoda species and one diplostraca species (as the outgroup) using two strategies, namely homology-based annotation and de novo annotation. The statistics and classification of TEs showed that their proportion in the genome and their taxonomic composition in decapoda were different. Moreover, correlation analysis and transcriptome data demonstrated that there were more PIF-Harbinger TEs in the genomes of Eriocheir sinensis and Scylla paramamosain, and the expression patterns of PIF-Harbingers were significantly altered under air exposure stress conditions. These results signaled that PIF-Harbingers expanded in the genome of E. sinensis and S. paramamosain and might be related to their air exposure tolerance levels. Meanwhile, sequence alignment revealed that some Jockey-like sequences (JLSs) with high similarity to specific regions of the White spot syndrome virus (WSSV) genome existed in all eight decapod species. At the same time, phylogenetic comparison exposed that the phylogenetic tree constructed by JLSs was not in agreement with that of the species tree, and the distribution of each branch was significantly different. The above-mentioned results signaled that these WSSV-specific JLSs might originate from the endogenesis of WSSV and were horizontally transferred via WSSV. This study accumulated data for expanding research on TEs in decapod species and also provided new insights and future direction for the breeding of stress-resistant and disease-resistant crab breeds.
Electrospun polysulfone hybrid nanocomposite fibers as membrane for separating oil/water emulsion
Authors: Ponnamma Deepalekshmi , Elgawady Yara , Hassan Mohamed , Adham Samer , Al-Maas Mashael , Alamgir Karim , Al-Maadeed Mariam 
Published: August 2023

Abstract
Commercial polymer membranes are largely utilized to separate oil/water mixtures, however membrane fouling, flux decline and short lifetime often inhibit their high performance. In order to resolve these drawbacks of the commercial membranes, we introduce a surface modification strategy following the electrospinning method. Electrospun fibers of polysulfone (PSf)/iron oxide (FeO)/halloysite nanotubes (HNT) nanocomposite is applied to modify the polyether sulfone (PES) standard membrane support surface for developing highly efficient oil/water emulsion separating membranes. This facile and simple spinning process for shorter periods ensures nanocomposite coatings on the standard PES membranes and allows better oil/water separation process. We analyze the structural and morphological characteristics of the modified membrane surface in addition to the hydrophilicity from contact angle studies. FeO nanoparticles of 2-5 nm and HNTs of <50 nm size mixed in PSf produce fibers of 531 ± 162 nm average diameter at a relatively lower applied electrical voltage 14.5 kV, compared to PSf. Under water and under oil contact angle values are used to prove the surface characteristics of the membranes and TOC values for the emulsion separation performance. From PES support to PSf and to PSf/HNT-FeO, the TOC values respectively change from 67 % to 75 % and to 79 %. We find the moderately hydrophilic membranes resulting in the higher permeate flux and quicker separation performance. We believe this study provides a notable solution to modify the surface of commercial membranes for better emulsion separation performance.
LINC01852 inhibits tumorigenesis and chemoresistance in colorectal cancer by regulating SRSF5-mediated PKM alternative splicing
Authors: Bian Zehua , Yang Fan , Xu Peiwen , Gao Ge , Yang Chunyu , Cao Yulin , Yao Surui , Wang Xue , Yin Yuan , Fei Bojian , Huang Zhaohui 
Published: August 2023

Abstract
Background Colorectal cancer (CRC) is a major cause of cancer-related deaths worldwide, and chemoresistance is a major obstacle in the treatment of this disease. Despite advances in therapy, the molecular mechanism underlying chemoresistance in CRC is not fully understood. Recent studies have implicated the key roles of long noncoding RNAs (lncRNAs) in the regulation of CRC chemoresistance.
Methods In this study, we investigated the role of lncRNA LINC01852 in CRC chemoresistance. The expression of LINC01852 was evaluated in multiple CRC cohorts using quantitative reverse transcription PCR. We conducted in vitro and in vivo functional experiments using cell culture and mouse models. RNA pull-down, RNA immunoprecipitation, chromatin immunoprecipitation, and dual luciferase assays were used to investigate the molecular mechanism of LINC01852 in CRC.
Results Our findings a lncRNA with tumor-inhibiting properties, LINC01852, that is downregulated in CRC and inhibits cell proliferation and chemoresistance both in vitro and in vivo. Further mechanistic investigations revealed that LINC01852 enhances TRIM72-mediated ubiquitination and degradation of SRSF5, inhibiting SRSF5-mediated PKM alternative splicing and thereby decreasing the production of PKM2. Overexpression of LINC01852 causes a metabolic switch from aerobic glycolysis to oxidative phosphorylation, which attenuates the chemoresistance of CRC by inhibiting PKM2-mediated glycolysis.
Conclusions Our results demonstrate that LINC01852 plays an important role in repressing CRC malignancy and chemoresistance by regulating SRSF5-mediated PKM alternative splicing, and that targeting the LINC01852/TRIM72/SRSF5/PKM2 signaling axis may represent a potential therapeutic strategy for the treatment of CRC.