bluuebunny/crossref_metadata_2025_split huggingface dataset:
The effect of doxapram on survival and APGAR score in newborn puppies delivered by elective caesarean: A randomized controlled trial
Authors: Hyndman Timothy H. , Fretwell Shelby , Bowden Ross S. , Coaicetto Flaminia , Irons Peter C. , Aleri Joshua W. , Kordzakhia Nino , Page Stephen W. , Musk Gabrielle C. , Tuke S. Jonathan , Mosing Martina , Metcalfe Steven S. 
Published: May 2023

AbstractDoxapram is marketed as a respiratory stimulant and is used by some veterinarians to help with neonatal apnoea, especially in puppies delivered by caesarean. There is a lack of consensus as to whether the drug is effective and data on its safety are limited. Doxapram was compared to placebo (saline) in newborn puppies in a randomized, double‐blinded clinical trial using two outcome measures: 7‐day mortality rate and repeated APGAR score measurements. Higher APGAR scores have been positively correlated with survival and other health outcomes in newborns. Puppies were delivered by caesarean and a baseline APGAR score was measured. This was immediately followed by a randomly allocated intralingual injection of either doxapram or isotonic saline (of the same volume). Injection volumes were determined by the weight of the puppy and each injection was administered within a minute of birth. The mean dose of doxapram administered was 10.65 mg/kg. APGAR scores were measured again at 2, 5, 10 and 20 min. One hundred and seventy‐one puppies from 45 elective caesareans were recruited into this study. Five out of 85 puppies died after receiving saline and 7 out of 86 died after receiving doxapram. Adjusting for the baseline APGAR score, the age of the mother and whether the puppy was a brachycephalic breed, there was insufficient evidence to conclude a difference in the odds of 7‐day survival for puppies that received doxapram compared to those that received saline (p = .634). Adjusting for the baseline APGAR score, the weight of the mother, the litter size, the mother's parity number, the weight of the puppy and whether the puppy was a brachycephalic breed, there was insufficient evidence to conclude a difference in the probability of a puppy having an APGAR score of ten (the maximum APGAR score) between those that received doxapram compared to those that received saline (p = .631). Being a brachycephalic breed was not associated with an increased odds of 7‐day mortality (p = .156) but the effect of the baseline APGAR score on the probability of having an APGAR score of ten was higher for brachycephalic than non‐brachycephalic breeds (p = .01). There was insufficient evidence that intralingual doxapram provided an advantage (or disadvantage) compared to intralingual saline when used routinely in puppies delivered by elective caesarean and that were not apnoeic.
Novel treatment of acute and acute‐on‐chronic liver failure: Interleukin‐22
Authors: Hwang Seonghwan , Hicks Amy , Hoo Chai Zhen , Kwon Yong Seong , Cho Ye Eun , Moore Joanna , Gao Bin 
Published: May 2023

AbstractAcute liver failure (ALF) is a life‐threatening medical condition, characterized by rapidly progressive hepatic dysfunction, coagulopathy and hepatic encephalopathy in patients without chronic liver disease, while acute‐on‐chronic liver failure (ACLF) occurs in patients with existing chronic liver disease. ALF and ACLF are often associated with multiple organ failure and a high short‐term mortality. In this review, we briefly discuss the causes and pathogenesis of ALF and ACLF, the current options available for the treatment of both deadly maladies and interleukin‐22 (IL‐22), a novel promising drug that may have great therapeutic potential for ALF and ACLF treatment. IL‐22 is a cytokine produced by immune cells but mainly targets epithelial cells including hepatocytes. IL‐22 has been shown to protect against organ damage and reduce bacterial infection in many preclinical models and several clinical trials including alcohol‐associated hepatitis. The potential application of IL‐22 for the treatment of ALF and ACLF is also elaborated.
The worldwide invasion history of a pest ambrosia beetle inferred using population genomics
Authors: Urvois T. , Perrier C. , Roques A. , Sauné L. , Courtin C. , Kajimura H. , Hulcr J. , Cognato A. I. , Auger‐Rozenberg M.‐A. , Kerdelhué C. 
Published: May 2023

AbstractXylosandrus crassiusculus, a fungus‐farming wood borer native to Southeastern Asia, is the most rapidly spreading invasive ambrosia species worldwide. Previous studies focusing on its genetic structure suggested the existence of cryptic genetic variation in this species. Yet, these studies used different genetic markers, focused on different geographical areas and did not include Europe. Our first goal was to determine the worldwide genetic structure of this species based on both mitochondrial and genomic markers. Our second goal was to study X. crassiusculus' invasion history on a global level and identify the origins of the invasion in Europe. We used a COI and RAD sequencing design to characterize 188 and 206 specimens worldwide, building the most comprehensive genetic data set for any ambrosia beetle to date. The results were largely consistent between markers. Two differentiated genetic clusters were invasive, albeit in different regions of the world. The markers were inconsistent only for a few specimens found exclusively in Japan. Mainland USA could have acted as a source for further expansion to Canada and Argentina through stepping stone expansion and bridgehead events. We showed that Europe was only colonized by Cluster II through a complex invasion history including several arrivals from multiple origins in the native area, and possibly including bridgehead from the United States. Our results also suggested that Spain was colonized directly from Italy through intracontinental dispersion. It is unclear whether the mutually exclusive allopatric distribution of the two clusters is due to neutral effects or due to different ecological requirements.
Evolutionary history of an island endemic, the Azorean common quail
Authors: Ravagni Sara , Sanchez‐Donoso Ines , Jiménez‐Blasco Irene , Andrade Pedro , Puigcerver Manel , Chorão Guedes Ana , Godinho Raquel , Gonçalves David , Leitão Manuel , Leonard Jennifer A. , Rodríguez‐Teijeiro José Domingo , Vilà Carles 
Published: May 2023

AbstractOceanic islands are characterized by conditions that favour diversification into endemic lineages that can be very different from their mainland counterparts. This can be the result of fast phenotypic divergence due to drift or the result of slower adaptation to local conditions. This uniqueness can obscure their evolutionary history. Here we used morphological, stable isotope, genetic and genomic data to characterize common quails (Coturnix coturnix) in the Azores archipelago and assess the divergence from neighbouring common quail populations. Historical documents suggested that these quails could have a recent origin associated with the arrival of humans in the last centuries. Our results show that Azorean quails constitute a well‐differentiated lineage with small size and dark throat pigmentation that has lost the migratory ability and that diverged from mainland quail lineages more than 0.8 mya, contrary to the notion of a recent human‐mediated arrival. Even though some Azorean quails carry an inversion that affects 115 Mbp of chromosome 1 and that has been associated with the loss of the migratory behaviour in other common quail populations, half of the analysed individuals do not have that inversion and still do not migrate. The long coexistence and evolution in isolation in the Azores of two chromosomal variants (with and without the inversion) is best explained by balancing selection. Thus, a unique and long evolutionary history led to the island endemic that we know today, C. c. conturbans.
Notes on [post]human nursing: What It MIGHT Be, What it is Not
Authors: Dillard‐Wright Jess , Smith Jamie B. , Hopkins‐Walsh Jane , Willis Eva , Brown Brandon B. , Tedjasukmana Emmanuel C. 
Published: May 2023

AbstractWith this paper, we walk out some central ideas about posthumanisms and the ways in which nursing is already deeply entangled with them. At the same time, we point to ways in which nursing might benefit from further entanglement with other ideas emerging from posthumanisms. We first offer up a brief history of posthumanisms, following multiple roots to several points of formation. We then turn to key flavors of posthuman thought to differentiate between them and clarify our collective understanding and use of the terms. This includes considerations of the threads of transhumanism, critical posthumanism, feminist new materialism, and the speculative, affirmative ethics that arise from critical posthumanism and feminist new materialism. These ideas are fruitful for nursing, and already in action in many cases, which is the matter we occupy ourselves with in the final third of the paper. We consider the ways nursing is already posthuman—sometimes even critically so—and the speculative worldbuilding of nursing as praxis. We conclude with visions for a critical posthumanist nursing that attends to humans and other/more/nonhumans, situated and material and embodied and connected, in relation.
Multiple paths of plant host toxicity are associated with the fungal toxin cercosporin
Authors: Koh Eugene , Chaturvedi Amit Kumar , Javitt Gabriel , Brandis Alexander , Fluhr Robert 
Published: May 2023

AbstractThe Cercospora species of fungi are responsible for leaf spot disease affecting many key economic crops. Most of these fungi secrete a toxic photodynamic molecule, cercosporin, that reacts with light and oxygen to produce reactive singlet oxygen (1O2) contributing to fungal virulence. We show similar cellular localization and aetiology of cercosporin in the non‐host Arabidopsis and the host Nicotiana benthamiana. Cercosporin accumulates in cell membranes in an oxidized state and in plastids in a mixture of redox states in a manner that is dependent on ongoing photosynthetic processes. We observed that cercosporin rapidly compromised photosynthesis as measured by Fv/Fm, NPQ, and photosystem I (PSI) parameters. Stomatal guard cells in particular demonstrated rapid light‐dependent membrane permeabilization that led to changes in leaf conductance. We showed that cercosporin‐mediated 1O2 generation oxidized RNA to form 8‐oxoguanosine (8‐oxoG), leading to translational attenuation and induction of 1O2 signature gene transcripts. We also identified a subset of cercosporin‐induced transcripts that were independent of the photodynamic effect. Our results point to the multimodal action of cercosporin that includes the inhibition of photosynthesis, the direct oxidation of nucleic acid residues and the elicitation of complex transcriptome responses.
The sound of liminality: MHD's afro trap, affective listening, and the (re)invention of Afropean identities in France
Authors: Niang Sophie Marie 
Published: May 2023

AbstractThis article is an exploration of the world making capacities of afro trap. Through an extended case study of MHD's discography, I ask what can be learnt about the liminal experiences of postcolonial black citizens, or Afropean citizens, in France, by listening to popular music. I argue that through embracing, reinventing, and (re)producing familiar Afropean soundscapes, MHD claims and creates from his liminal subject position. Going against the assumption that Frenchness and blackness are always mutually exclusive and in tension, this music sonically proposes a way of being otherwise in France, stemming from this liminality. I see in what I call ‘the sound of liminality’ an instance of ‘queering ethnicity,’ one which channels the affective capacities of sound. I propose affective listening as a method that, incorporating autoethnography to consider critical listening positionality, facilitates a renewed attention to sound as an object of sociological inquiry.
Experimental and Numerical Investigation of Thermal Performance of an Air-Cooled Battery Module Under High Ambient Temperature Conditions
Authors: Sharma Dinesh Kumar , Prabhakar Aneesh 
Published: May 2023

Abstract
Lithium-ion batteries (LiBs) are widely used in electric vehicles due to their high energy and power density. The operating temperature has a significant impact on the thermal performance and longevity of LiBs. The thermal performance of an air-cooled battery module containing 16 (4S4P) high-energy density LiBs has been investigated through a series of experiments and numerical simulations. At varying transverse and longitudinal cell spacing, airflow rates, ambient temperatures, and discharge C-rates, the thermal performance of a battery module with aligned battery cells was analyzed. For the thermal performance evaluation, the average temperature rise, temperature non-uniformity, and maximum temperature of the module’s battery cells are utilized. During discharge cycles, the rate of temperature increase is linear but becomes nonlinear at the end of the discharge cycle. In the current architecture of the battery module, a minimum space utilization ratio of 0.38 is necessary to limit maximum temperature and temperature non-uniformity to safe battery thermal management temperatures. The thermal performance was significantly affected by the airflow rate. Increasing airflow rate decreases temperature but increases pressure drop substantially. The maximum cell temperature is greatly affected by the inlet air temperature, increasing from 62.8 °C to 76.6 °C when the inlet air temperature is increased from 30 °C to 45 °C. At high ambient temperatures (over 40 °C), LiB temperatures exceed permissible limits, and air cooling alone is inadequate. This study examines the thermal performance of an air-cooled battery module working at high temperatures.
Rhizosphere microbial community composition shifts diurnally and in response to natural variation in host clock phenotype
Authors: Hubbard Charley J. , Harrison Joshua G. , McMinn Robby , Bennett Ponsford Julian C. , Maignien Lois , Ewers Brent , Weinig Cynthia 
Published: May 2023

ABSTRACT

Plant-associated microbial assemblages are known to shift at time scales aligned with plant phenology, as influenced by the changes in plant-derived nutrient concentrations and abiotic conditions observed over a growing season. But these same factors can change dramatically in a sub-24-hour period, and it is poorly understood how such diel cycling may influence plant-associated microbiomes. Plants respond to the change from day to night via mechanisms collectively referred to as the internal “clock,” and clock phenotypes are associated with shifts in rhizosphere exudates and other changes that we hypothesize could affect rhizosphere microbes. The mustard
Boechera stricta
has wild populations that contain multiple clock phenotypes of either a 21- or a 24-hour cycle. We grew plants of both phenotypes (two genotypes per phenotype) in incubators that simulated natural diel cycling or that maintained constant light and temperature. Under both cycling and constant conditions, the extracted DNA concentration and the composition of rhizosphere microbial assemblages differed between time points, with daytime DNA concentrations often triple what were observed at night and microbial community composition differing by, for instance, up to 17%. While we found that plants of different genotypes were associated with variation in rhizosphere assemblages, we did not see an effect on soil conditioned by a particular host plant circadian phenotype on subsequent generations of plants. Our results suggest that rhizosphere microbiomes are dynamic at sub-24-hour periods, and those dynamics are shaped by diel cycling in host plant phenotype.

IMPORTANCE
We find that the rhizosphere microbiome shifts in composition and extractable DNA concentration in sub-24-hour periods as influenced by the plant host’s internal clock. These results suggest that host plant clock phenotypes could be an important determinant of variation in rhizosphere microbiomes.
COVID-19 seroprevalence among local authority workers from Orléans Métropole, the Community of Communes of the Terres du Val de Loire, the local public service management centre of the Loiret department and the Region Centre Val de Loire: a prospective epidemiological study
Authors: Hanane Guerrout , Amine Zaouia , Roomila Naeck , Prazuck Thierry , Amirouche Ammar , Olivier Vernay , Benyamina Amine , Serreau Raphaël 
Published: May 2023

ObjectiveTo evaluate the SARS-CoV-2 seroprevalence among local authority workers, depending on their position and potential interaction with the public.MethodsA cohort of volunteer participants was recruited among local authority workers of the Centre Val de Loire region in France, to be tested using a rapid serological test (COVID-PRESTO). The collected data were analysed by comparing different parameters including, gender, age, position held, and contact or not with the public. The study was carried out from August to December 2020 and included 3228 participants (n=3228), from 18 to 65 years old.ResultsThe seroprevalence of SARS-CoV-2 among local authority workers was estimated at 3.04%. No significant difference could be observed according to the position held by the workers and whether they were or not in contact with the public. Nevertheless, a significant difference was observed between the different investigating centres, in correlation with the geographical location.ConclusionContact with members of the public was not a critical parameter for SARS-CoV-2 seroprevalence as long as protective measures are applied. Among the population included in the study, childcare workers were more at risk of getting infected by the virus.Trial registration numberNCT04387968
Validation of the TRI-SCORE in patients undergoing surgery for isolated tricuspid regurgitation
Authors: Anguita-Gámez María , Giraldo María A , Nombela-Franco Luis , Eixeres Esteve Andrea , Cuerpo Gregorio , Lopez-Menendez Jose , Gomez-Polo Juan Carlos , Tirado-Conte Gabriela , Torres Berenice , Pedraz Prieto Alvaro , Jimenez-Quevedo Pilar , Lopez-Gude María Jesus , Mahia Patricia , Cobiella Javier , Zamorano José Luis , Vilacosta Isidre , Maroto Luis , Carnero-Alcázar Manuel 
Published: May 2023

IntroductionEstimation of peri-procedural risk in patients with tricuspid regurgitation (TR) undergoing isolated tricuspid valve surgery (ITVS) is of paramount importance. The TRI-SCORE is a new surgical risk scale specifically developed for this purpose, which ranged from 0 to 12 points and included eight parameters: right-sided heart failure signs, daily dose of furosemide ≥125 mg, glomerular filtration rate <30 mL/min, elevated bilirubin (with a value of 2 points), age ≥70 years, New York Heart Association Class III–IV, left ventricular ejection fraction <60% and moderate/severe right ventricular dysfunction (with a value of 1 point). The objective of the study was to evaluate the performance of the TRI-SCORE in an independent cohort of patients undergoing ITVS.MethodsA retrospective observational study was performed in four centres, including consecutive adult patients undergoing ITVS for TR between 2005 and 2022. The TRI-SCORE and the traditional risk scores used in cardiac surgery (Logistic EuroScore (Log-ES) and EuroScore-II (ES-II)) were applied for each patient, and discrimination and calibration of the three scores were evaluated in the entire cohort.ResultsA total of 252 patients were included. The mean age was 61.5±11.2 years, 164 (65.1%) patients were female, and TR mechanism was functional in 160 (63.5%) patients. The observed in-hospital mortality was 10.3%. The estimated mortality by the Log-ES, ES-II and TRI-SCORE was 8.7±7.3%, 4.7±5.3% and 11.0±16.6%, respectively. Patients with a TRI-SCORE ≤4 and >4 had an in-hospital mortality of 1.3% and 25.0%, p=0.001, respectively. The discriminatory capacity of the TRI-SCORE had a C-statistic of 0.87 (0.81–0.92), which was significantly higher than both the Log-ES (0.65 (0.54–0.75)) and ES-II (0.67 (0.58–0.79)), p=0.001 (for both comparisons).ConclusionThis external validation of the TRI-SCORE demonstrated good performance to predict in-hospital mortality in patients undergoing ITVS, which was significantly better than the Log-ES and ES-II, which underestimated the observed mortality. These results support the widespread use of this score as a clinical tool.
The NR_109/FUBP1/c-Myc axis regulates TAM polarization and remodels the tumor microenvironment to promote cancer development
Authors: Zhang Cong , Wei Sisi , Dai Suli , Li Xiaoya , Wang Huixia , Zhang Hongtao , Sun Guogui , Shan Baoen , Zhao Lianmei 
Published: May 2023

BackgroundTumor-associated macrophages (TAMs) are a major component of the tumor microenvironment (TME) and exert an important role in tumor progression. Due to the heterogeneity and plasticity of TAMs, modulating the polarization states of TAMs is considered as a potential therapeutic strategy for tumors. Long noncoding RNAs (lncRNAs) have been implicated in various physiological and pathological processes, yet the underlying mechanism on how lncRNAs manipulate the polarization states of TAMs is still unclear and remains to be further investigated.MethodsMicroarray analyses were employed to characterize the lncRNA profile involved in THP-1-induced M0, M1 and M2-like macrophage. Among those differentially expressed lncRNAs, NR_109 was further studied, for its function in M2-like macrophage polarization and the effects of the condition medium or macrophages mediated by NR_109 on tumor proliferation, metastasis and TME remodeling both in vitro and in vivo. Moreover, we revealed how NR_109 interacted with far upstream element-binding protein 1 (FUBP1) to regulate the protein stability through hindering ubiquitination modification by competitively binding with JVT-1. Finally, we examined sections of tumor patients to probe the correlation among the expression of NR_109 and related proteins, showing the clinical significance of NR_109.ResultsWe found that lncRNA NR_109 was highly expressed in M2-like macrophages. Knockdown NR_109 impeded IL-4 induced M2-like macrophage polarization and significantly reduced the activity of M2-like macrophages to support the proliferation and metastasis of tumor cells in vitro and in vivo. Mechanistically, NR_109 competed with JVT-1 to bind FUBP1 at its C-terminus domain, impeded the ubiquitin-mediated degradation of FUBP1, activatedc-Myctranscription and thus promoted M2-like macrophages polarization. Meanwhile, as a transcription factor, c-Myc could bind to the promoter of NR_109 and enhance the transcription of NR_109. Clinically, high NR_109 expression was found in CD163+TAMs from tumor tissues and was positively correlated with poor clinical stages of patients with gastric cancer and breast cancer.ConclusionsOur work revealed for the first time that NR_109 exerted a crucial role in regulating the phenotype-remodeling and function of M2-like macrophages via a NR_109/FUBP1/c-Myc positive feedback loop. Thus, NR_109 has great translational potentials in the diagnosis, prognosis and immunotherapy of cancer.