bluuebunny/crossref_metadata_2025_split huggingface dataset:
Surgical outcomes of sacrospinous ligament fixation at the time of vaginal hysterectomy for vaginal vault prolapse prevention: 10 years review
Authors: Bunyavejchevin Suvit , Songsiriphan Athiwat , Ruanphoo Purim , Chiengthong Keerati 
Published: May 2023

AbstractAimsTo study the non‐recurrent prolapse rate after vaginal hysterectomy colpoperineorrhaphy and sacrospinous ligament fixation (SSLF) with or without anterior colporrhaphy, with or without posterior colpoperineorrhaphy.Materials and MethodsA retrospective cohort study was carried out at King Chulalongkorn Memorial Hospital, Bangkok, Thailand, during January 1, 2012—December 31, 2021. The inclusion criteria were Thai women diagnosed with uterovaginal prolapse and undergone vaginal hysterectomy, with or without anterior colporrhaphy, with or without posterior colpoperineorrhaphy, with SSLF and had the complete pelvic organ prolapse quantification (POP‐Q) information at the 12 months follow up. The recurrent case was defined as any compartment prolapse ≥ stage II by POP‐Q classification at 1, 3, 6, 12 months follow up.ResultsOf 71 cases included, 20 cases had recurrent prolapse at 12 months. The incidences of non‐recurrent prolapse rate at 1, 3, 6, and 12 months were 70/71 (98.59%), 65/70 (92.86%), 58/65 (89.23%), and 51/58 (87.93%). The overall PHVP prevalence at 12 months was 2/71 (2.81%). The anterior compartment prolapse were 18/71 (25.35%) at 12 months. The most common postoperative complications were the urinary tract infection (4.23%). There were one case of nerve entrapment and one case of stitches exposure that required stitches removal.ConclusionSSLF at the time of vaginal hysterectomy is safe and effective in post hysterectomy vaginal vault prolapse prevention. But there are high recurrence rate of anterior compartment prolapse at 12 months. Preoperative counseling and long‐term follow up after SSLF is advocated.
Exploring practices of multiliteracies pedagogy through digital technologies: a narrative inquiry
Authors: Yap Jia Rong , Gurney Laura 
Published: May 2023

AbstractDigital technologies have fast become integral within literacy learning and teaching across contexts as students engage with a variety of digital and multimodal texts. While teachers in New Zealand schools have a high degree of autonomy in the design and planning of literacy programs, little is currently known about how they understand and enact multiliteracies pedagogy (MLP). Using data gathered via interviews and classroom observations in an intermediate school in New Zealand, this article adopts a narrative inquiry approach to explore one teacher's approaches to using digital technologies and texts within literacy instruction. We explore in particular the ways in which MLP may be enactedimplicitlyrather than explicitly, within the complex matrix of teachers' personal beliefs and learning experiences, the perceived learning needs of students, and the school curriculum. We conclude with a call for the conscious and purposeful teaching of MLP, focusing on synaesthesia and the semiotic functions of texts.
Characterization of iron meteorites by scanning electron microscopy, X‐ray diffraction, magnetization measurements, and Mössbauer spectroscopy: Gibeon IVA
Authors: Goryunov M. V. , Varga G. , Dankházi Z. , Felner I. , Chukin A. V. , Kuzmann E. , Homonnay Z. , Grokhovsky V. I. , Oshtrakh M. I. 
Published: May 2023

AbstractGibeon IVA iron meteorite fragment was characterized using optical microscopy, scanning electron microscopy (SEM) with energy dispersive spectroscopy (EDS), X‐ray diffraction (XRD), magnetization measurements, and Mössbauer spectroscopy. Optical microscopy and SEM made on the polished section of the meteorite, show the presence of α‐Fe(Ni, Co) and γ‐Fe(Ni, Co) phases and plessite structures. There are no troilite inclusions observed in the studied section. EDS studies indicate some variations in the Ni concentrations: (i) within the α‐Fe(Ni, Co) phase in the range ~5.0 ± 0.1 – ~7.5 ± 0.1 at% and (ii) within the γ‐Fe(Ni, Co) phase in the range ~26.0 ± 0.2 – ~36.1 ± 0.2 at%. The latter Ni concentration range indicates the presence of small amount of the paramagnetic γ‐phase in addition to the ferromagnetic γ‐phase. EDS also shows that Ni content in two plessite structures is varying in the range ~16–37 at%, which can indicate the presence of only the α2‐Fe(Ni, Co) and γ‐Fe(Ni, Co) phases in the duplex plessite structure. This may be a result of the γ‐phase decomposition with the incomplete martensitic transformation: γ → α2 + γ due to a faster cooling rate. XRD indicates the presence of ~1.3 wt% of the γ‐Fe(Ni, Co) phase in Gibeon VIA. The saturation magnetization moment of 185(2) emu g−1 obtained also confirms the presence of phases with low and high Ni concentrations. The most appropriate fit of the Gibeon IVA Mössbauer spectrum demonstrates the presence of five magnetic sextets and one paramagnetic singlet which are assigned to the ferromagnetic α2‐Fe(Ni, Co), α‐Fe(Ni, Co), γ‐Fe(Ni, Co), and paramagnetic γ‐Fe(Ni, Co) phases. The relative average Fe contents in these phases are: 13.4% in the α2‐Fe(Ni, Co) phase, 78.3% in the α‐Fe(Ni, Co) phase, and 8.3% in the ferromagnetic and paramagnetic γ‐Fe(Ni, Co) phases.
Enhanced neuron–glia network in the submucosa and increased neuron outgrowth into the mucosa are associated with distinctive expressions of neuronal factors in the colon of rat IBS model
Authors: Fujikawa Yoshiko , Tominaga Kazunari 
Published: May 2023

AbstractBackgroundNeuronal attraction and repulsion factors regulate neuron network formation. In the colon of irritable bowel syndrome (IBS), neuron network and enteric glial cells (EGCs) in the submucosa, neuronal outgrowth in the mucosa, and expressions of neuronal factors remain unknown.MethodsIBS models were prepared by intracolonic injections of acetic acid to Wistar Kyoto (WKY) rats. Using whole‐mount submucosal plexus tissue stripped from the distal colon, we examined neuron network, EGC morphology, and localization of both attraction factor (nerve growth factor: NGF) and repulsion factor (semaphorin3A: Sema3A). We evaluated mRNA expressions of NGF and Sema3A in the mucosa and submucosa and neuron outgrowth into the mucosa.Key ResultsIn IBS models, nerve fibers were thickened and densely increased in the submucosa remarkably from the outer toward the inner plexus. Submucosal EGCs exhibited process hyperplasia and bulbous swelling of terminals. NGF was predominantly expressed in EGCs than neurons in the submucosa. NGF mRNA expressions were increased in the submucosa in WKY, and their expressions were increased in the mucosa after the injection. Sema3A mRNA expressions were increased in both layers of WKY but tended to be decreased in the mucosa alone after the injection. Neuron outgrowth was increased into the mucosa. NGF was localized at EGCs in the lamina propria mucosae but not mucosal mast cells.Conclusions & InferencesNeuron network enhancement in the submucosa and neuron outgrowth into the mucosa may be associated with axon guidance factors expressed in hyperplastic EGCs in the colonic submucosa of IBS models.
The AP2/ERF transcription factor TOE4b regulates photoperiodic flowering and grain yield per plant in soybean
Authors: Li Haiyang , Du Haiping , Huang Zerong , He Milan , Kong Lingping , Fang Chao , Chen Liyu , Yang Hui , Zhang Yuhang , Liu Baohui , Kong Fanjiang , Zhao Xiaohui 
Published: May 2023

SummaryPhotoperiod‐mediated flowering determines the phenological adaptability of crops including soybean (Glycine max). A genome‐wide association study (GWAS) identified a new flowering time locus, Time of flowering 13 (Tof13), which defined a gene encoding an AP2/ERF transcription factor. This new transcription factor, which we named TOE4b, is localized in the nucleus. TOE4b has been selected for soybean latitude adaptability. The existing natural variant TOE4bH4 was rare in wild soybean accessions but occurred more frequently in landraces and cultivars. Notably, TOE4bH4 improved high‐latitude adaptation of soybean to some extent. The gene‐edited TOE4b knockout mutant exhibited earlier flowering, conversely, TOE4b overexpression delayed flowering time. TOE4b is directly bound to the promoters and gene bodies of the key flowering integration factor genes FT2a and FT5a to inhibit their transcription. Importantly, TOE4b overexpression lines in field trials not only showed late flowering but also altered plant architecture, including shorter internode length, more internodes, more branches and pod number per plant, and finally boosted grain yield per plant by 60% in Guangzhou and 87% in Shijiazhuang. Our findings therefore identified TOE4b as a pleiotropic gene to increase yield potential per plant in soybean, and these results provide a promising option for breeding a soybean variety with an idealized plant architecture that promotes high yields.
Metabolomic and transcriptomic analyses reveal the biosynthetic mechanisms of pigments and main taste compounds in an albino tea cultivar
Authors: Liu Sheng‐Chuan , Xu Ying‐Fen , Liu Ya‐Bing , Zhao Xiong , Wei Jie , Lin Kai‐Qin , Liu Yan , Yan Dong‐Hai 
Published: May 2023

AbstractSignificant variations in leaf colours, pigment contents, and main taste compounds in young shoots from albino tea plants (Camellia sinensis) influence tea flavour. However, the seasonal metabolic pattern and molecular regulatory mechanism of these metabolites remain largely elusive. Herein, we conducted morphological, biochemical, metabolomic and transcriptomic analyses between an albino tea cultivar ‘Zhonghuang 3’ (‘ZH3’) and a green strain ‘Tai cha 15’ (‘TC15’) at four‐time points (April 12, May 31, July 14, and August 17) to elucidate dynamic changes in these compounds and predict the relationships among transcription factors (TFs), target genes (TGs), and metabolite abundance. Generally, leaf colours and pigment contents were significantly lighter and lower, respectively, in ‘ZH3’ than in ‘TC15’ from spring to summer, but were subsequently similar. Compared to ‘TC15’, ‘ZH3’ had a lower and broader phenol/ammonia ratio as well as stable caffeine content and showed more significantly different metabolites and differentially expressed genes. The relationship between pigments, main taste compounds, and their biosynthetic genes, as well as TFs and their TGs, had genetic specificity. These results suggested that the biosynthesis of these compounds was probably both season‐ and variety‐dependent. In total, 12 models of the TF‐TG‐metabolite regulatory network were proposed to uncover the biosynthetic and regulatory mechanisms of these metabolites in tea plants. A high correlation was observed between some structural genes and TFs with the accumulation of these metabolites. These findings provide novel insights into the regulatory mechanisms underlying accumulation of pigments and main taste compounds in tea plants.
Validation of the g.tec Unicorn Hybrid Black wireless EEG system
Authors: Pontifex Matthew B. , Coffman Colt A. 
Published: May 2023

AbstractAlthough dry and hybrid‐style electrode technology has been well validated, systems utilizing these electrodes have not been widely adopted. One reason for this may be that the systems incorporating such technology present limitations that are fundamental to the EEG approach. The g.tec Unicorn Hybrid Black system, a low density Bluetooth EEG amplifier, however, attempts to address many of these limitations to allow greater flexibility to replicate methods used with traditional EEG amplifiers and extend them to more novel applications. The aim of the present investigation was to validate the g.tec Unicorn Hybrid Black amplifier to determine if it provides comparable data to a traditional laboratory‐based system when no electrode preparation is utilized or if a saline‐based solution is necessary to obtain sufficient signal quality. Stimulus‐locked ERPs and EEG power spectrum data were concurrently recorded using both the Unicorn Hybrid Black amplifier and a traditional high‐end laboratory‐based low‐impedance wired system. Findings suggest that the Unicorn Hybrid Black provides valid measures for investigations of frequency spectra even with no conductive solution applied. However, to obtain valid assessments of event‐related brain potentials, it appears necessary to use a conductive solution for electrode preparation. This system appears well suited to allow for high‐quality and flexible EEG measures available outside of traditional laboratory environments.
Proof of concept for detection of staphylococcal enterotoxins in platelet concentrates as a novel safety mitigation strategy
Authors: Chi Sylvia Ighem , Yousuf Basit , Paredes Carina , Bearne Jennifer , McDonald Carl , Ramirez‐Arcos Sandra 
Published: May 2023

AbstractBackground and ObjectivesStaphylococcus aureus is a predominant contaminant of platelet concentrates (PCs) that can evade detection during screening with culture methods. Importantly, S. aureus produces staphylococcal enterotoxins (SEs) during PC storage, which are linked to slow growth and enhanced biofilm formation. This study investigated timing of SE production during PC storage and feasibility of SE detection as a PC safety strategy.Materials and MethodsGenomic and transcriptomic data of transfusion‐relevant S. aureus PS/BAC/169/17/W, PS/BAC/317/16/W, CI/BAC/25/13/W and CBS2016‐05 were used to determine the presence and differential expression of exotoxin genes in PCs. Trypticase soy broth (TSB) and PCs were inoculated with 1.0E+06 cfu/mL of S. aureus PS/BAC/169/17/W and CBS2016‐05. Expression of SEs at different growth phases was confirmed with Western blotting. PCs were inoculated with 30 cfu/unit of the same strains, and SE detection during PC storage was optimized with a sandwich dot‐ELISA assay.ResultsS. aureus genomes contain multiple exotoxin genes including those encoding for SEs. Transcriptome data revealed significant upregulation (0.5–6.7‐fold, p < 0.05) of SE genes in PCs versus TSB. Western blots demonstrated SE production at all growth phases. Notably, dot‐ELISA detected clinically relevant concentrations of SEs (~0.2 μg/mL) at 32 h of PC storage when S. aureus PS/BAC/169/17/W and CBS2016‐05 counts were 1.8E+04 and 1.4E+04 cfu/mL, respectively.ConclusionGenomic analyses revealed that staphylococcal exotoxins are widely distributed and highly conserved among transfusion‐relevant S. aureus isolates. Furthermore, SEs are significantly upregulated in PCs and detected at 30 h of PC storage. Therefore, bacterial toxin detection could supplement mitigation strategies to enhance PC safety.
Coaching Elementary Teachers in Literacy: Does Feedback Type Matter for Low Implementers?
Authors: Cutrer-Párraga Elizabeth A. , Miller Erica Ellsworth , Gjini Erjola , Garrett Melia Fonoimoana 
Published: May 2023

For literacy coaches and teachers, feedback is an integral component of effective coaching. Yet, little is understood about the interaction between feedback and high/low implementing teachers within coaching sessions. This multiple case study explored the types of feedback literacy coaches provided both high and low implementing teachers over a two-year period. In the first year, the literacy coaches provided at least twice as much instructional feedback as emotional feedback to low-implementing teachers. Those teachers not only received more feedback in general, but the feedback they received was primarily what they needed to do to improve. In contrast, during the same period literacy coaches provided high-implementing teachers more emotional feedback than instructional feedback. Low implementation, or resistance, observed in this study may have been tied to the overwhelming amount and type of feedback the low implementing teachers received. Coaches seeking to enhance instructional practices could benefit from attending to the amount and type of feedback they provide their learners.
Nephrologists’ perspectives on the impact of COVID-19 on caring for patients undergoing dialysis in Latin America: a qualitative study
Authors: Matus Gonzalez Andrea , Lorca Eduardo , Cabrera Sebastian , Hernandez Alejandra , Zúñiga-SM Carlos , Sola Laura , Michea Luis , Ferreiro Fuentes Alejandro , Cervantes Lilia , Madero Magdalena , Teixeira-Pinto Armando , Wong Germaine , Craig Jonathan , Jaure Allison 
Published: May 2023

ObjectiveTo describe the experiences of nephrologists on caring for patients undergoing in-centre haemodialysis during the COVID-19 pandemic in Latin America.DesignTwenty-five semistructured interviews were conducted by Zoom videoconference in English and Spanish languages during 2020 until data saturation. Using thematic analysis, we conducted line-by-line coding to inductively identify themes.Setting25 centres across nine countries in Latin America.ParticipantsNephrologists (17 male and 8 female) were purposively sampled to include diverse demographic characteristics and clinical experience.ResultsWe identified five themes: shock and immediate mobilisation for preparedness (overwhelmed and distressed, expanding responsibilities to manage COVID-19 infection and united for workforce resilience); personal vulnerability (being infected with COVID-19 and fear of transmitting COVID-19 to family); infrastructural susceptibility of dialysis units (lacking resources and facilities for quarantine, struggling to prevent cross-contamination, and depletion of personal protective equipment and cleaning supplies); helplessness and moral distress (being forced to ration life-sustaining equipment and care, being concerned about delayed and shortened dialysis sessions, patient hesitancy to attend to dialysis sessions, being grieved by socioeconomic disparities, deterioration of patients with COVID-19, harms of isolation and inability to provide kidney replacement therapy); and fostering innovative delivery of care (expanding use of telehealth, increasing uptake of PD and shifting focus on preventing syndemics).ConclusionNephrologists felt personally and professionally vulnerable and reported feeling helpless and morally distressed because they doubted their capacity to provide safe care for patients undergoing dialysis. Better availability and mobilisation of resources and capacities to adapt models of care, including telehealth and home-based dialysis, are urgently needed.
PANDORA dyadic project: hope, spiritual well-being and quality of life of dyads of patients with chronic obstructive pulmonary disease in Switzerland – a multicentre longitudinal mixed-methods protocol study
Authors: Baptista Peixoto Befecadu Filipa , Stirnemann Jerome , Guerreiro Ivan , Fusi-Schmidhauser Tanja , Jaksic Cyril , Larkin Philip J , da Rocha Rodrigues Gora , Pautex Sophie 
Published: May 2023

IntroductionChronic obstructive pulmonary disease (COPD) is responsible for 2.9 million deaths annually in Europe. Symptom burden and functional decline rise as patients reach advanced stages of the disease enhancing risk of vulnerability and dependency on informal caregivers (ICs).Evidence shows that hope is an important psycho-social-spiritual construct that humans use to cope with symptom burden and adversity. Hope is associated with increased quality of life (QoL) comfort and well-being for patients and ICs. A better understanding of the meaning and experience of hope over time as patients transition through chronic illness may help healthcare professionals to plan and deliver care more appropriately.Methods and analysisThis is a longitudinal multicentre mixed-methods study with a convergent design. Quantitative and qualitative data will be collected from dyads of advanced COPD patients and their ICs in two university hospitals at two points in time. The Herth Hope Index, WHO Quality of Life BREF, Functional Assessment of Chronic Illness Therapy-Spiritual Well-being and the French version of the Edmonton Symptom Assessment Scale will be used to collect data. Dyadic interviews will be conducted using a semi-structured interview guide with five questions about hope and their relationship with QoL.Statistical analysis of data will be carried out using R V.4.1.0. To test whether our theoretical model as a whole is supported by the data, structural equation modelling will be used. The comparison between T1 and T2 for level of hope, symptom burden, QoL and spiritual well-being, will be carried out using paired t-tests. The association between symptom burden, QoL, spiritual well-being and hope will be tested using Pearson correlation.Ethics and disseminationThis study protocol received ethical approval on 24 May 2022 from theCommission cantonale d'éthique de la recherche sur l'être humain—Canton of Vaud. The identification number is 2021-02477.
Effect of environmental enrichment on relapse rates in patients with severe alcohol use disorder: protocol for a randomised controlled trial
Authors: Barillot Lila , Chauvet Claudia , Besnier Marc , Jaafari Nematollah , Solinas Marcello , Chatard Armand 
Published: May 2023

IntroductionAlcohol use disorder (AUD) ranks among the most prevalent psychiatric disorders worldwide. Despite current treatments, more than half of patients relapse within weeks after treatment. In animal models, exposure to environmental enrichment (EE) has been shown to be a promising approach to reduce relapse. However, controlled, multimodal EE is difficult to transpose to humans. To address this gap, this study aims at assessing the effectiveness of exposure to a newly designed EE protocol during AUD treatment in reducing relapse to alcohol use. Our EE will allow an enhancement of the standard intervention, and will combine several promising enrichment factors identified in the literature—physical activity, cognitive stimulation, mindfulness and virtual reality (VR).Methods and analysisA randomised controlled trial involving 135 participants receiving treatment for severe AUD will be conducted. Patients will be randomised to an intervention enhancement group or a control group. The enhanced intervention will consist of six 40-min sessions of EE spread over 9 days. During the first 20 min of these sessions, patients will practise mindfulness in multisensory VR, in virtual environments designed to practise mindfulness and use it to regulate craving induced by virtual cues or stress. Then, participants will practise indoor cycling combined with cognitive training exercises. The control group will undergo standard management for AUD. The primary outcome is relapse assessed at 2 weeks after treatment, using a questionnaire and biological indicators. Relapse will be defined as drinking at least five drinks per occasion or drinking at least five times a week. It is predicted that the group receiving the EE intervention will have a lower relapse rate than the control group. The secondary outcomes are relapse at 1 month and 3 months after treatment, craving and drug-seeking behaviour, mindfulness skills acquisition and the effect of the intervention enhancement on the perceived richness of the daily environment, assessed by questionnaires and neuropsychological tasks.Ethics and disseminationAll participants have to give written informed consent to the investigator. This study is approved by the Ethics Committee Nord Ouest IV of Lille (reference number 2022-A01156-37). Results will be disseminated through presentations, peer-reviewed journals and seminar conferences. All information on ethical considerations and open science practices can be accessed athttps://osf.io/b57uj/Trial registration numberNCT05577741.