bluuebunny/crossref_metadata_2025_split huggingface dataset:
Dynamic in situ growth of bonded-phase silica nanospheres on silica capillary inner walls for open-tubular liquid chromatography
Authors: Ahmed Mohamed Adel , Ghiasvand Alireza , Quirino Joselito P. 
Published: June 2023

Abstract
Silica nanospheres (SNS) were grown on the inner walls of silica capillaries through a dynamic in situ nucleation process to prepare a highly porous and large accessible surface area substrate. The SNS were then functionalized with octadecyl (C18), 3-aminopropyltriethoxysilane (APTES), beta-cyclodextrin (β-CD), and amino groups to develop robust and efficient chromatographic stationary phases. The modified silica capillaries were exploited for open-tubular liquid chromatography (OT-LC) and open-tubular capillary electrochromatography (OT-CEC) applications. The prepared stationary phases were compared to conventional capillaries in terms of separation performance. The synthesis process was optimized, and the bonded-phase stationary phases were characterized by the electron microscopy technique. The effects of different solvents, additives, and functional groups on the geometry and chromatographic resolving power of the SNS were envisaged. The capillaries modified with octadecyl groups were evaluated for the separation of non-steroidal anti-inflammatory drugs, phenones, alkenylbenzenes, and enantiomers of chlorophenoxy herbicides. As an application instance, an SNS-C18-coated capillary was utilized for the separation of alkenylbenzenes from clove extract and protein digest medium, through OT-LC and OT-CEC techniques, respectively. The β-CD functionalized capillary was applied for the OT-CEC separation of a dichlorprop racemic mixture.
Graphical abstract
The RIG-I agonist M8 triggers cell death and natural killer cell activation in human papillomavirus-associated cancer and potentiates cisplatin cytotoxicity
Authors: Girone Carlo , Calati Federica , Lo Cigno Irene , Salvi Valentina , Tassinari Valentina , Schioppa Tiziana , Borgogna Cinzia , Lospinoso Severini Ludovica , Hiscott John , Cerboni Cristina , Soriani Alessandra , Bosisio Daniela , Gariglio Marisa 
Published: June 2023

AbstractAlthough the activation of innate immunity to treat a wide variety of cancers is gaining increasing attention, it has been poorly investigated in human papillomavirus (HPV)-associated malignancies. Because these tumors harbor a severely impaired cGAS-STING axis, but they still retain a largely functional RIG-I pathway, another critical mediator of adaptive and innate immune responses, we asked whether RIG-I activation by the 5’ppp-RNA RIG-I agonist M8 would represent a therapeutically viable option to treat HPV+ cancers. Here, we show that M8 transfection of two cervical carcinoma-derived cell lines, CaSki and HeLa, both expressing a functional RIG-I, triggers intrinsic apoptotic cell death, which is significantly reduced in RIG-I KO cells. We also demonstrate that M8 stimulation potentiates cisplatin-mediated cell killing of HPV+ cells in a RIG-I dependent manner. This combination treatment is equally effective in reducing tumor growth in a syngeneic pre-clinical mouse model of HPV16-driven cancer, where enhanced expression of lymphocyte-recruiting chemokines and cytokines correlated with an increased number of activated natural killer (NK) cells in the tumor microenvironment. Consistent with a role of RIG-I signaling in immunogenic cell killing, stimulation of NK cells with conditioned medium from M8-transfected CaSki boosted NK cell proliferation, activation, and migration in a RIG-I-dependent tumor cell-intrinsic manner. Given the highly conserved molecular mechanisms of carcinogenesis and genomic features of HPV-driven cancers and the remarkably improved prognosis for HPV+ oropharyngeal cancer, targeting RIG-I may represent an effective immunotherapeutic strategy in this setting, favoring the development of de-escalating strategies.
Agriculture and crop dispersal in the western periphery of the Old World: the Amazigh/Berber settling of the Canary Islands (ca. 2nd–15th centuries ce)
Authors: Morales Jacob , Speciale Claudia , Rodríguez-Rodríguez Amelia , Henríquez-Valido Pedro , Marrero-Salas Efrain , Hernández-Marrero Juan Carlos , López Rosa , Delgado-Darias Teresa , Hagenblad Jenny , Fregel Rosa , Santana Jonathan 
Published: June 2023

AbstractThe Canary Islands were settled ca. 1,800 years ago by Amazigh/Berber farming populations originating in North Africa. This historical event represents the last and westernmost expansion of the Mediterranean farming package in Antiquity, and investigating it yields information about crop dispersal along the periphery of the Mediterranean world around the turn of the first millennium ce. The current study focuses on archaeobotanical evidence recorded in a series of pre-Hispanic/Amazigh sites of the Canary Islands (ca. 2nd–15th centuries ce). It offers new, unpublished archaeobotanical findings and direct radiocarbon datings of plant remains from the different islands. The general goal is to gain a better grasp of how the first settlers of the Canary Islands adapted their farming activities to the different natural conditions of each island. The results suggest a shared crop ‘package’ throughout the islands since at least the 3rd–5th centuries ce. This set of plants was likely introduced from north-western Africa and consists of Hordeum vulgare (hulled barley), Triticum durum (durum wheat), Lens culinaris (lentil), Vicia faba (broad bean), Pisum sativum (pea), and Ficus carica (fig). The crop ‘package’ probably arrived in a single episode during the initial colonisation and was not followed by any other plants. Subsequent to the initial settling and until the arrival of the European seafarers, the islands remained isolated from each other and from the outside world, a condition that over time led to a decline in crop diversity in all of the islands except Gran Canaria.
Leading by example in a public goods experiment with benefit heterogeneity
Authors: Yu Jing , Kocher Martin G. 
Published: June 2023

AbstractSocial dilemmas such as greenhouse gas emission reduction are often characterized by heterogeneity in benefits from solving the dilemma. How should leadership of group members be organized in such a setting? We implement a laboratory public goods experiment with heterogeneous marginal per capita returns from the public good and leading by example that is either implemented exogenously or by self-selection. Our results suggest that both ways of implementing leadership only have small effects on contributions to the public good. Self-selected leaders—in particular self-selected low-benefit leaders—tend to set better examples than imposed leaders, but they are also exploited more strongly by followers. Leaders seem to need additional instruments to be more effective when benefits are heterogeneous.
Treatment patterns of individualized real-life tapering approaches based on shared decision-making in rheumatoid arthritis
Authors: Birkner Benjamin , Rech Jürgen , Edelmann Edmund , Verheyen Frank , Schett Georg , Stargardt Tom 
Published: June 2023

Abstract
Objective
To provide real-world evidence on patient-individual tapering patterns of disease-modifying antirheumatic drugs (DMARDs) in rheumatoid arthritis (RA) patients in daily clinical practice.

Methods
Data obtained through a controlled prospective cohort study in Germany conducted from July 2018 to March 2021 were analyzed. Participants consist of RA patients in sustained remission who were eligible for DMARD tapering at enrolment. Data from RA patients who experienced tapering of DMARDs at least once during the observational period (n = 200) were used. Descriptive analyses of medical outcomes at baseline and at time of first tapering, time to first tapering, tapering patterns by substance group, and tapering intensity were documented.

Results
We did not observe meaningful differences in either disease activity or quality of life measures between substance groups at enrolment, time of first tapering, and at 6 or 12 months after tapering. Median time until first tapering varied between substance groups (csDMARDs: 108 days; bDMARDs: 189 days; combination: 119 days). Most patients received one iteration of tapering only (147/200 patients, 73.5%). Dose reduction was applied for patients treated with csDMARDs (79/86 patients, 91.8%), spacing of interval was the most frequent strategy for patients treated with bDMARDs only (43/48 patients, 89.5%). Necessity for increased DMARD dosage was observed in only 10% of patients (20/200). Tapering intensity by substance was overall heterogenous, indicating high individualization.

Conclusion
We identify highly heterogeneous tapering patterns between substance groups and within substances. Identification and recognition of patient-individual approaches of tapering will help to further improve the management of RA for both patients and rheumatologists.
Assessment of diagnostic reference levels for paediatric cardiac computed tomography in accordance with European guidelines
Authors: Aboul Hamad Mohamed S. , Attalla Ehab M. , Amer Hanan H. , Fathy Mohamed M. 
Published: June 2023

AbstractRecently, paediatric cardiac computed tomography (CCT) has caused concerns that diagnostic image quality and dose reduction may require further improvement. Consequently, this study aimed to establish institutional (local) diagnostic reference levels (LDRLs) for CCT for paediatric patients, and assess the impact of tube voltage on proposed DRLs in terms of the volume computed tomography index (CTDIvol) and dose length product (DLP). In addition, effective doses (EDs) of exposure were estimated. A population of 453 infants, whose mass and age were less than 12 kg and 2 years, respectively, were considered from January 2018 to August 2021. Based on previous studies, this number of patients was considered to be sufficient for establishing LDRLs. A group of 245 patients underwent CCT examinations at 70 kVp tube voltage with an average scan range of 23.4 cm. Another set of 208 patients underwent CCT examinations at 100 kVp tube voltage with an average scan range of 15.8 cm. The observed CTDIvol and DLP values were 2.8 mGy and 54.8 mGy.cm, respectively. The mean effective dose (ED) was 1.2 mSv. It is concluded that provisional establishment and use of DRLs for cardiac computed tomography in children are crucial, and further research is needed to develop regional and international DRLs.
Economic evaluation of Motor Neuron Diseases: a nationwide cross-sectional analysis in Germany
Authors: Heinrich Felix , Cordts Isabell , Günther René , Stolte Benjamin , Zeller Daniel , Schröter Carsten , Weyen Ute , Regensburger Martin , Wolf Joachim , Schneider Ilka , Hermann Andreas , Metelmann Moritz , Kohl Zacharias , Linker Ralf A. , Koch Jan Christoph , Radelfahr Florentine , Schönfelder Erik , Gardt Pavel , Mohajer-Peseschkian Tara , Osmanovic Alma , Klopstock Thomas , Dorst Johannes , Ludolph Albert C. , Schöffski Oliver , Boentert Matthias , Hagenacker Tim , Deschauer Marcus , Lingor Paul , Petri Susanne , Schreiber-Katz Olivia 
Published: June 2023

Abstract
Background and objectives
Motor Neuron Diseases (MND) are rare diseases but have a high impact on affected individuals and society. This study aims to perform an economic evaluation of MND in Germany.

Methods
Primary patient-reported data were collected including individual impairment, the use of medical and non-medical resources, and self-rated Health-Related Quality of Life (HRQoL). Annual socio-economic costs per year as well as Quality-Adjusted Life Years (QALYs) were calculated.

Results
404 patients with a diagnosis of Amyotrophic Lateral Sclerosis (ALS), Spinal Muscular Atrophy (SMA) or Hereditary Spastic Paraplegia (HSP) were enrolled. Total annual costs per patient were estimated at 83,060€ in ALS, 206,856€ in SMA and 27,074€ in HSP. The main cost drivers were informal care (all MND) and disease-modifying treatments (SMA). Self-reported HRQoL was best in patients with HSP (mean EuroQoL Five Dimension Five Level (EQ-5D-5L) index value 0.67) and lowest in SMA patients (mean EQ-5D-5L index value 0.39). QALYs for patients with ALS were estimated to be 1.89 QALYs, 23.08 for patients with HSP and 14.97 for patients with SMA, respectively. Cost-utilities were estimated as follows: 138,960€/QALY for ALS, 525,033€/QALY for SMA, and 49,573€/QALY for HSP. The main predictors of the high cost of illness and low HRQoL were disease progression and loss of individual autonomy.

Conclusion
As loss of individual autonomy was the main cost predictor, therapeutic and supportive measures to maintain this autonomy may contribute to reducing high personal burden and also long-term costs, e.g., care dependency and absenteeism from work.
The neuroanatomical hallmarks of chronic tinnitus in comorbidity with pure-tone hearing loss
Authors: Elmer Stefan , Schmitt Raffael , Giroud Nathalie , Meyer Martin 
Published: June 2023

AbstractTinnitus is one of the main hearing impairments often associated with pure-tone hearing loss, and typically manifested in the perception of phantom sounds. Nevertheless, tinnitus has traditionally been studied in isolation without necessarily considering auditory ghosting and hearing loss as part of the same syndrome. Hence, in the present neuroanatomical study, we attempted to pave the way toward a better understanding of the tinnitus syndrome, and compared two groups of almost perfectly matched individuals with (TIHL) and without (NTHL) pure-tone tinnitus, but both characterized by pure-tone hearing loss. The two groups were homogenized in terms of sample size, age, gender, handedness, education, and hearing loss. Furthermore, since the assessment of pure-tone hearing thresholds alone is not sufficient to describe the full spectrum of hearing abilities, the two groups were also harmonized for supra-threshold hearing estimates which were collected using temporal compression, frequency selectivity und speech-in-noise tasks. Regions-of-interest (ROI) analyses based on key brain structures identified in previous neuroimaging studies showed that the TIHL group exhibited increased cortical volume (CV) and surface area (CSA) of the right supramarginal gyrus and posterior planum temporale (PT) as well as CSA of the left middle-anterior part of the superior temporal sulcus (STS). The TIHL group also demonstrated larger volumes of the left amygdala and of the left head and body of the hippocampus. Notably, vertex-wise multiple linear regression analyses additionally brought to light that CSA of a specific cluster, which was located in the left middle-anterior part of the STS and overlapped with the one found to be significant in the between-group analyses, was positively associated with tinnitus distress level. Furthermore, distress also positively correlated with CSA of gray matter vertices in the right dorsal prefrontal cortex and the right posterior STS, whereas tinnitus duration was positively associated with CSA and CV of the right angular gyrus (AG) and posterior part of the STS. These results provide new insights into the critical gray matter architecture of the tinnitus syndrome matrix responsible for the emergence, maintenance and distress of auditory phantom sensations.
Honey for acute cough in children — a systematic review
Authors: Kuitunen Ilari , Renko Marjo 
Published: June 2023

AbstractTo analyze the efficacy of using honey to treat acute cough in children. Systematic review, synthesis without meta-analysis. We searched PubMed, Scopus, CENTRAL, CINAHL, and Web of Science databases on August 15, 2022, for words honey and cough. Randomized controlled trials conducted in children were included. Risk-of-bias and evidence quality were assessed. Studies were not pooled due to lack of key information. Instead, we provided the range of observed effects for the main outcomes. Three hundred ninety-six papers were screened, and 10 studies were included. Two studies had high risk-of-bias and six had some concerns. Honey seemed to decrease cough frequency more than placebo/no treatment (range of observed effect 0.0–1.1 points) and cough medication (0.2–0.9 points). Sleep improved more often in the honey group (range of effect was 0.0–1.1) compared to placebo/no treatment and (− 0.2–1.1 points) compared to cough medication. Quality of the evidence was low to very low.Conclusion: We found low quality evidence that honey may be more effective than cough medication or placebo/no treatment in relieving symptoms and improving sleep in children with acute cough. Better quality randomized, placebo-controlled blinded trials are needed to confirm the effectiveness of honey in treating acute cough in children.Trial registration: CRD42022369577.

What is Known:• Honey has been suggested to be effective as a symptomatic treatment in acute cough.• Prior randomized trials have had conflicting results and thus an overview of the literature was warranted.

What is New:• Based on low quality evidence honey may be more effective than placebo or over-the-counter medications for acute symptom reliwef in cough.• Future studies with better reporting are needed to confirm the results.
A proposed difficulty grading system for laparoscopic bile duct exploration: benefits to clinical practice, training and research
Authors: Nassar Ahmad H. M. , Sallam Mahmoud , Khan Khurram S. , Kilpatrick Rhona , Zino Samer , Katbeh Tarek Z. 
Published: June 2023

Abstract
Background
A gap remains between the mounting evidence for single session management of bile duct stones and the adoption of this approach. Laparoscopic bile duct exploration (LBDE) is limited by the scarcity of training opportunities and adequate equipment and by the perception that the technique requires a high skill-set. The aim of this study was to create a new classification of difficulty based on operative characteristics and to stratify postoperative outcomes of easy vs. difficult LBDE irrespective of the surgeon’s experience.

Methods
A cohort of 1335 LBDEs was classified according to the location, number and size of ductal stones, the retrieval technique, utilisation of choledochoscopy and specific biliary pathologies encountered. A combination of features indicated easy (Grades I and II A & B) or difficult (Grades III A and B, IV and V) transcystic or transcholedochal explorations.

Results
78.3% of patients with acute cholecystitis or pancreatitis, 37% with jaundice and 46% with cholangitis had easy explorations. Difficult explorations were more likely to present as emergencies, with obstructive jaundice, previous sphincterotomy and dilated bile ducts on ultrasound scans. 77.7% of easy explorations were transcystic and 62.3% of difficult explorations transductal. Choledochoscopy was utilised in 23.4% of easy vs. 98% of difficult explorations. The use of biliary drains, open conversions, median operative time, biliary-related complications, hospital stay, readmissions, and retained stones increased with the difficulty grade. Grades I and II patients had 2 or more hospital episodes in 26.5% vs. 41.2% for grades III to V. There were 2 deaths in difficulty Grade V and one in Grade IIB.

Conclusion
Difficulty grading of LBDE is useful in predicting outcomes and facilitating comparison between studies. It ensures fair structuring and assessment of training and progress of the learning curve. LBDEs were easy in 72% with 77% completed transcystically. This may encourage more units to adopt this approach.

Graphical abstract
Association between lifestyle modifications and improvement of early cardiac damage in children and adolescents with excess weight and/or high blood pressure
Authors: Genovesi Simonetta , Tassistro Elena , Giussani Marco , Antolini Laura , Lieti Giulia , Orlando Antonina , Montemerlo Massimo , Patti Ilenia , Parati Gianfranco 
Published: June 2023

Abstract

Background
It is not known whether, in children and adolescents with alterations in weight and/or blood pressure (BP), lifestyle modifications are associated with an improvement of early cardiac damage.

Methods
In a pediatric population referred for excess weight, high BP, or both (n = 278, 10.6 (2.3) years), echocardiography was performed at enrollment and after 15 months of follow-up, during which participants received nonpharmacological treatment, based on correcting unhealthy lifestyles and improving dietary habits. Left ventricular mass was indexed for height (g/m2.7, LVMI), and an LVMI value higher than or equal to age- and gender-specific 95th percentile was the criterion for defining left ventricular hypertrophy (LVH). Multiple linear and logistic regression analyses were carried out to determine associations between changes in BMI and BP z-scores and changes of LVMI values and LVH prevalence, from baseline to follow-up.

Results
At baseline, 33.1% of study participants were hypertensive, 52.9% obese, and 36.3% had LVH. At follow-up, the prevalence of hypertension, obesity, and LVH was 18.7%, 30.2%, and 22.3%, respectively (p < 0.001 for all). A decrease in LVMI from 37.1 to 35.2 g/m2.7 (p < 0.001) was observed. Only delta BMI z-score positively related to an improvement of LVMI. Reductions of BMI (OR = 0.22, 95% CI 0.07–0.64) and diastolic BP (OR = 0.64, 95% CI 0.42–0.93) z-scores from baseline to follow-up and family history of hypertension (OR = 0.36, 95% CI 0.16–0.78) were associated with a lower prevalence of LVH.

Conclusions
In a pediatric population at cardiovascular risk, changing incorrect lifestyle and dietary habits is associated with both reduction of BMI and BP values and regression of early cardiac damage.

Graphical abstract
DeepGraviLens: a multi-modal architecture for classifying gravitational lensing data
Authors: Pinciroli Vago Nicolò Oreste , Fraternali Piero 
Published: June 2023

AbstractGravitational lensing is the relativistic effect generated by massive bodies, which bend the space-time surrounding them. It is a deeply investigated topic in astrophysics and allows validating theoretical relativistic results and studying faint astrophysical objects that would not be visible otherwise. In recent years, machine learning methods have been applied to support the analysis of the gravitational lensing phenomena by detecting lensing effects in datasets consisting of images associated with brightness variation time series. However, the state-of-the-art approaches either consider only images and neglect time-series data or achieve relatively low accuracy on the most difficult datasets. This paper introduces DeepGraviLens, a novel multi-modal network that classifies spatio-temporal data belonging to one non-lensed system type and three lensed system types. It surpasses the current state-of-the-art accuracy results by $$\approx 3\%$$

≈
3
%

to $$\approx 11\%$$

≈
11
%

, depending on the considered data set. Such an improvement will enable the acceleration of the analysis of lensed objects in upcoming astrophysical surveys, which will exploit the petabytes of data collected, e.g., from the Vera C. Rubin Observatory.