bluuebunny/crossref_metadata_2025_split huggingface dataset:
Collagen and actin network mediate antiviral immunity against Orsay in C. elegans intestinal cells
Authors: Zhou Ying , Zhong Weiwei , Tao Yizhi Jane 
Published: April 2023

ABSTRACTC. elegans is a free-living nematode that is widely used as a small animal model for studying fundamental biological processes and disease mechanisms. Since the discovery of the Orsay virus in 2011, C. elegans also holds the promise of dissecting virus-host interaction networks and innate antiviral immunity pathways in an intact animal. Orsay primarily targets the worm intestine, causing enlarged intestinal lumen as well as visible changes to infected cells such as liquefaction of cytoplasm and rearrangement of the terminal web. Previous studies of Orsay identified that C. elegans is able to mount antiviral responses by DRH-1/RIG-I mediated RNA interference and Intracellular Pathogen Response, a uridylyltransferase that destabilizes viral RNAs by 3′ end uridylation, and ubiquitin protein modifications and turnover. To comprehensively search for novel antiviral pathways inC. elegans, we performed genome-wide RNAi screens by bacterial feeding using existing bacterial RNAi libraries covering 94% of the entire genome. Out of the 106 antiviral genes identified, we investigated those in three new pathways: collagens, actin remodelers, and epigenetic regulators. By characterizing Orsay infection in RNAi and mutant worms, our results indicate that collagens likely form a physical barrier in intestine cells to inhibit viral infection by preventing Orsay entry. Furthermore, evidence suggests that the intestinal actin (act-5), which is regulated by actin remodeling proteins (unc-34,wve-1andwsp-1), a Rho GTPase (cdc-42) and chromatin remodelers (nurf-1andisw-1), also provides antiviral immunity against Orsay possibly through another physical barrier presented as the terminal web.
Glycolytic reprogramming fuels myeloid cell-driven hypercoagulability
Authors: Rehill Aisling M. , Leon Gemma , McCluskey Sean , Schoen Ingmar , Hernandez-Santana Yasmina , Annett Stephanie , Klavina Paula , Robson Tracy , Curtis Annie M. , Renné Thomas , Hussey Seamus , O’Donnell James S. , Walsh Patrick T. , Preston Roger J.S. 
Published: April 2023

ABSTRACTBackgroundMyeloid cell metabolic reprogramming is a hallmark of inflammatory disease, however, its role in inflammation-induced hypercoagulability is poorly understood.Objective/MethodsUsing novel myeloid cell-based global haemostasis assays and murine models of immunometabolic disease, we evaluated the role of inflammation-associated metabolic reprogramming in regulating blood coagulation.ResultsGlycolysis was essential for enhanced activated myeloid cell tissue factor expression and decryption, driving increased cell-dependent thrombin generation in response to inflammatory challenge. Similarly, inhibition of glycolysis enhanced activated macrophage fibrinolytic activity via reduced plasminogen activator inhibitor 1 (PAI-1)-activity. Macrophage polarisation or activation markedly increased endothelial protein C receptor (EPCR) expression on monocytes and macrophages, leading to increased myeloid cell-dependent protein C activation. Importantly, inflammation-dependent EPCR expression on tissue-resident macrophages was also observedin vivo. Adipose tissue macrophages from obese mice fed a high-fat diet exhibited significantly enhanced EPCR expression and APC generation compared to macrophages isolated from the adipose tissue of healthy mice. Similarly, the induction of colitis in mice prompted infiltration of EPCR+innate myeloid cells within inflamed colonic tissue that were absent from the intestinal tissue of healthy mice.ConclusionCollectively, this study identifies immunometabolic regulation of myeloid cell hypercoagulability, opening new therapeutic possibilities for targeted mitigation of thrombo-inflammatory disease.ESSENTIALSInflammation-mediated glycolytic reprogramming enables myeloid cell-induced hypercoagulability and antifibrinolytic activity.2-Deoxy-D-glucose (2-DG) inhibits the expression of transcription factors necessary for inflammation-induced procoagulant gene expression.Myeloid cell membrane regulation of tissue factor procoagulant activity is glycolysis-dependent.Activation of myeloid innate immunity dysregulates activated protein C anticoagulant pathway activity.
Structure and assembly of a bacterial gasdermin pore
Authors: Johnson Alex G. , Mayer Megan L. , Schaefer Stefan L. , McNamara-Bordewick Nora K. , Hummer Gerhard , Kranzusch Philip J. 
Published: April 2023

AbstractIn response to pathogen infection, gasdermin (GSDM) proteins form membrane pores that induce a host cell death process called pyroptosis1–3. Studies of human and mouse GSDM pores reveal the functions and architectures of 24–33 protomers assemblies4–9, but the mechanism and evolutionary origin of membrane targeting and GSDM pore formation remain unknown. Here we determine a structure of a bacterial GSDM (bGSDM) pore and define a conserved mechanism of pore assembly. Engineering a panel of bGSDMs for site-specific proteolytic activation, we demonstrate that diverse bGSDMs form distinct pore sizes that range from smaller mammalian-like assemblies to exceptionally large pores containing >50 protomers. We determine a 3.3 Å cryo-EM structure of aVitiosangiumbGSDM in an active slinky-like oligomeric conformation and analyze bGSDM pores in a native lipid environment to create an atomic-level model of a full 52-mer bGSDM pore. Combining our structural analysis with molecular dynamics simulations and cellular assays, our results support a stepwise model of GSDM pore assembly and suggest that a covalently bound palmitoyl can leave a hydrophobic sheath and insert into the membrane before formation of the membrane-spanning β-strand regions. These results reveal the diversity of GSDM pores found in nature and explain the function of an ancient post-translational modification in enabling programmed host cell death.
Enhanced Peripheral Nerve Regeneration by Mechano-electrical Stimulation
Authors: Tai Youyi , Tonmoy Thamidul Islam , Win Shwe , Brinkley Natasha T. , Hyle Park B. , Nam Jin 
Published: April 2023

AbstractTo address limitations in current approaches for treating large peripheral nerve defects, this study evaluated the efficacy of functional material-mediated physical stimuli on peripheral nerve regeneration. Electrospun piezoelectric poly(vinylidene fluoride-trifluoroethylene) nanofibers were utilized to deliver mechanical actuation-activated electrical stimulation to nerve cells/tissues in a non-invasive manner. Using morphologically and piezoelectrically optimized nanofibers for neurite extension and Schwann cell maturation based on in vitro experiments, piezoelectric nerve conduits were implanted in a rat sciatic nerve transection model to bridge a critical-sized sciatic nerve defect (15 mm). A therapeutic shockwave system was utilized to activate the piezoelectric effect of the implanted nerve conduit on demand. The piezoelectric nerve conduit-mediated mechano-electrical stimulation induced enhanced peripheral nerve regeneration, resulting in full axon reconnection with myelin regeneration from the proximal to the distal ends over the critical-sized nerve gap. Furthermore, superior functional recovery was observed by walking track analysis and polarization-sensitive optical coherence tomography, demonstrating the excellent efficacy of the mechano-electrical stimulation strategy for treating peripheral nerve injuries.
Virally induced lipid droplets are a platform for innate immune signalling complexes
Authors: Monson EA , Laws JL , Telikani Z , Milligan AJ , Rozario AM , Amarasinghe I , Smith ML , Tran V , Dinh Q , Williamson N , Mechler A , Johnson C , Hofer MJ , Nie S , Whelan DR , Helbig KJ 
Published: April 2023

AbstractLipid droplets (LDs) are upregulated by host cells in the face of pathogen infection, however, the reason for this phenomenon remains largely unknown. Here, we demonstrate that virally induced LDs house a distinct and dynamic proteome containing key antiviral signalling pathway members, including the essential pattern recognition receptor; RIG-I, key adaptor proteins; STAT1 and STAT2 and prominent interferon inducible proteins; viperin and MX1. Changes in the LD proteome were underpinned by specific key changes in the lipidome of virally driven LDs, particularity in the phospholipid membrane. Following virus infection, key antiviral proteins formed complex protein-protein interactions on the LD surface, positioning this organelle as a key antiviral signalling platform for the first time. It is clear that dynamic regulation of both the proteome and the lipidome of LDs occurs rapidly following viral infection towards the initiation of a successful innate immune response.
Multiplexed Sequential Imaging in Living Cells with Orthogonal Fluorogenic RNA Aptamer/Dye Pairs
Authors: Zheng Ru , Wu Rigumula , Liu Yuanchang , Sun Zhining , Bagheri Yousef , Xue Zhaolin , Mi Lan , Tian Qian , Pho Raymond , Siddiqui Sidrat , Ren Kewei , You Mingxu 
Published: April 2023

AbstractSingle-cell detection of multiple target analytes is an important goal in cell biology. However, due to the spectral overlap of common fluorophores, multiplexed fluorescence imaging beyond two-to-three targets inside living cells remains a technical challenge. Herein, we introduce a multiplexed imaging strategy that enables live-cell target detection via sequential rounds of imaging-and-stripping process, which is named as “sequential Fluorogenic RNA Imaging-Enabled Sensor” (seqFRIES). In seqFRIES, multiple orthogonal fluorogenic RNA aptamers are genetically encoded inside cells, and then the corresponding cell membrane permeable dye molecules are added, imaged, and rapidly removed in consecutive detection cycles. As a proof-of-concept, we have identified in this study fivein vitroorthogonal fluorogenic RNA aptamer/dye pairs (>10-fold higher fluorescence signals), four of which can be used for highly orthogonal and multiplexed imaging in living bacterial and mammalian cells. After further optimizing the cellular fluorescence activation and deactivation kinetics of these RNA/dye pairs, the whole four-color semi-quantitative seqFRIES process can now be completed in ∼20 min. Meanwhile, seqFRIES-mediated simultaneous detection of two critical signaling molecules, guanosine tetraphosphate and cyclic diguanylate, was also achieved within individual living cells. We expect our validation of this new seqFRIES concept here will facilitate the further development and potential broad usage of these orthogonal fluorogenic RNA/dye pairs for highly multiplexed and dynamic cellular imaging and cell biology studies.
A Logic-incorporated Gene Regulatory Network Deciphers Principles in Cell Fate Decisions
Authors: Xue Gang , Zhang Xiaoyi , Li Wanqi , Zhang Lu , Zhang Zongxu , Zhou Xiaolin , Zhang Di , Zhang Lei , Li Zhiyuan 
Published: April 2023

AbstractOrganisms utilize gene regulatory networks (GRNs) to make fate decisions, but the regulatory mechanisms of transcription factors (TFs) in GRNs are exceedingly intricate. A longstanding question in this field is how these tangled interactions synergistically contribute to decision- making procedures. To comprehensively understand the role of regulatory logic in cell fate decisions, we constructed a logic-incorporated GRN model and examined its behavior under two distinct driving forces (noise-driven and signal-driven). Under the noise-driven mode, we distilled the relationship among fate bias, regulatory logic, and noise profile. Under the signal-driven mode, we bridged regulatory logic and progression-accuracy trade-off, and uncovered distinctive trajectories of reprogramming influenced by logic motifs. In differentiation, we characterized a special logic-dependent priming stage by the solution landscape. Finally, we applied our findings to decipher three biological instances: hematopoiesis, embryogenesis, and trans-differentiation. Orthogonal to the classical analysis of expression profile, we harnessed noise patterns to construct the GRN corresponding to fate transition. Our work presents a generalizable framework for top- down fate-decision studies and a practical approach to the taxonomy of cell fate decisions.
COMPLETE GENOME SEQUENCE OFVIBRIO SYNGNATHI SP. NOV., A FISH PATHOGEN, ISOLATED FROM THE KIEL FJORD
Authors: Chibani Cynthia Maria , Hertel Robert , Neumann-Schaal Meina , Goehlich Henry , Wagner Kim , Bunk Boyke , Spröer Cathrin , Overmann Jörg , Hoppert Michael , Marten Mareike , Roth Olivia , Liesegang Heiko , Wendling Carolin C. 
Published: April 2023

AbstractA newVibriostrain K08M4Twas isolated from the broad-nosed pipefishSyngnathus typhlein the Kiel Fjord. Infection experiments revealed that K08M4Tis highly virulent for juvenile pipefish. Cells of strain K08M4Tare Gram-stain-negative, curved rod-shaped and motile by means of a single polar flagellum. The strain can grow aerobically at 9 to 40°C, at pH 4 to 10.5 and tolerates up to 12% (w/v) NaCl. The most prevalent (> 10%) cellular fatty acids of K08M4Twere C16:1ω7cand C16:0.Whole-genome comparisons revealed that K08M4Trepresents a separate evolutionary lineage which is distinct from otherVibriospecies and falls within theVibrio Splendidusclade. The genome is 4,886,292 bp in size, consists of two circular chromosomes (3,298,328 bp, 1,587,964 bp), and comprises 4,178 protein-coding genes and 175 RNA genes. In this study, we describe the phenotypic features of the new isolate and present the annotation and analysis of its complete genome sequence. Based on these data, the new isolate represents a new species for which we propose the nameVibrio syngnathi. The type strain is K08M4T(=DSM 109818T).Supplementary materialOne supplementary figure and six supplementary table are available with the online version of the Manuscript.
Analysis of the worldwide diversity ofXanthomonas hortorumpv.carotae, the agent of bacterial blight of carrot, reveals two distinct populations
Authors: Dupas Enora , Durand Karine , Jacques Marie-Agnès 
Published: April 2023

AbstractXanthomonas hortorumpv.carotaeis a pathogen responsible for the bacterial blight of carrot. While it infects carrot fields worldwide, its diversity remains unknown. Here, we validated by PCR the identification asX. hortorumpv.carotaeof most of the strains isolated from carrot symptoms. We study their diversity by sequencing seven housekeeping genes. The analysis confirmed the identity of most of the strains previously identified asX. hortorumpv.carotaeand highlighted the presence of two clusters inside the pathovarcarotae. The 18 non-X. hortorumpv.carotaehave been identified asXanthomonasfrom other species. As theX. hortorumpv.carotaestrains clustered only in two groups by this analysis, a new MLVA scheme was developed. It allowed to validate the subclusteration in two groups and observe diversity among them. At the same times based on this study and data from the literature, we proposed the strain CFBP 7900 as the neopathotype strain of the pathovar. The strain should replacement the holopathotype strain CFBP 4997, as it is no longer available in international collection.
A gender-comparative study of informal entrepreneurship: the moderating role of location decision
Authors: Silupu Brenda , Amorós José Ernesto , Usero Belen , Montoro-Sánchez Ángeles 
Published: April 2023

PurposeMotivations and access to resources for venturing differ between men and women. In developing countries, there has been an increase in businesses that do not have a specific location and persist in informality. This research aimed to evaluate, from a gender perspective, the moderating effect of the decision not to have a place in the relationship between human capital (education, experience and type of entrepreneurship) and business informality.Design/methodology/approachUsing the National Household Survey 2014–2018, a sample of 50,313 Peruvian entrepreneurs was obtained − 23,314 women and 27,002 men – who have been in business for over three years. The data were analysed with logistic regression.FindingsThe results showed a moderating effect of entrepreneurship without a settled location on the relationship between education and informality in the case of women. And, for men, the moderating impact falls on the education, experience and reason for venturing that influences the formality of their businesses.Originality/valueThe problem of business informality of established companies with more than 42 months of operation is analysed. The moderating effect of the decision not to have a specific location on the relationship between human capital and informality is explored. This work extends business informality studies in Latin America developing countries, incorporating a gender perspective.
Structure of REDEE and EJMBE research: a bibliometric analysis
Authors: Ramos Ricardo , Rita Paulo 
Published: April 2023

PurposeEvaluating existing literature can lead to a better understanding of a scientific journal's state of the art. In this sense, this study aims to analyze the global research evolution of the Revista Europea de Dirección y Economia de la Empresa (REDEE) and the European Journal of Management and Business Economics (EJMBE).Design/methodology/approachA bibliometric analysis was conducted to acknowledge the most contributing authors, impactful articles, publication trends, keyword analysis, co-occurrence networks and collaboration networks. A total of 454 articles published between 2006 and 2022 were analyzed.FindingsThe results suggest that the international strategy set in 2014 has resulted in a steadily growing number of publications and a significant increment in citations. Relationship marketing and the connections between innovation, performance and entrepreneurship are topics of interest for the EJMBE.Originality/valueMapping existing EJMBE research through identifying the contributing authors, most impactful articles, publication trends, keyword analysis, co-occurrence networks and collaboration networks is missing to encourage new research projects.
Distance learning during COVID-19 school closures
Authors: Hean Oudom , Chairassamee Nattanicha 
Published: April 2023

PurposeThe authors aim to analyze the inequality in accessing distance learning during COVID-19 school closures.Design/methodology/approachThe authors use the Household Pulse Survey, which is an effort by the United States (US) government to measure the well-being of American families during the COVID-19 pandemic. The authors employ a regression analysis to estimate the inequality in accessing distance learning by race and household income.FindingsDisadvantaged children from nonwhite and low-income families have much less access to distance learning, including less access to online classes, digital devices and the Internet. Schools are critical providers of the Internet and digital devices to children from disadvantaged households. Schools and parents devote more attention to these nonwhite children by spending extra time on their learning activities.Originality/valueThis paper estimates the inequality in accessing distance learning during the COVID-19 crisis. Also, the authors analyze the responses of schools and parents toward this inequality.
How green human resource management affects employee voluntary workplace green behaviour: An integrated model
Authors: Yuan Yiwei , Ren Shuang , Tang Guiyao , Ji Haochen , Cooke Fang Lee , Wang Zhining 
Published: April 2023

AbstractGreen human resource management (GHRM), a set of HRM practices targeted at environmental goals, has been proposed as the key to achieving organisational sustainable development. However, the mechanisms through which GHRM influences employee green behaviour are not yet well understood. Drawing on conservation of resources theory, this study presents an integrated model revealing the mixed effects of GHRM on employees' voluntary workplace green behaviour (VWGB). Path analysis based on two studies undertaken in China largely supported our hypotheses. Specifically, GHRM was found to positively influence employees' VWGB through environmental commitment, while simultaneously decreasing their VWGB through emotional exhaustion. Meanwhile, supervisory support for environmental behaviour mitigated the impact of GHRM on emotional exhaustion as well as the relationship between GHRM and employee VWGB via emotional exhaustion. This study contributes to the GHRM literature in particular and organisational environmental management literature in general.
Survival and medical costs of non‐small cell lung cancer patients according to the first‐line treatment: An observational study using the Kyoto City Integrated Database
Authors: Shimamoto Tomonari , Tateyama Yukiko , Kobayashi Daisuke , Yamamoto Keiichi , Takahashi Yoshimitsu , Ueshima Hiroaki , Sasaki Kosuke , Nakayama Takeo , Iwami Taku 
Published: April 2023

AbstractBackgroundLung cancer is the primary cause of cancer mortality and non‐small cell lung cancer (NSCLC) accounts for the majority of lung cancer cases. New drug treatments have been developed since 2010 but there are concerns about the increase in medical costs. This study aimed to compare survival and medical costs among patients with NSCLC according to their initial treatment to estimate the impact of early NSCLC detection.MethodsPatients with primary NSCLC who filed insurance claims between April 2013 and March 2019 were identified using the Kyoto City Integrated Database. Patients were divided into two groups depending on their initial treatment: the resection group and drug or radiation group. The survival and medical costs were calculated.ResultsA total of 2609 patients with primary NSCLC were identified. Among them, 1035 patients underwent resection. The 5‐year survival was 75% for the resection group while below 25% for the drug or radiation group. At 6 months of survival, the median cumulative total cost was 2409 thousand yen (interquartile range [IQR] 1947–4012 thousand yen) in the resection group and 2951 thousand yen (IQR 1600–4706 thousand yen) in the drug or radiation group. At 4 years of survival, the cumulative median total cost was 5257 thousand yen (IQR 3808–8243 thousand yen) in the resection group and 10 202 thousand yen (IQR 4845–20 450 thousand yen) in the drug or radiation group.ConclusionsAs a first‐line therapy in newly diagnosed patients with NSCLC, surgical resection is associated with longer survival and lower medical costs than pharmacotherapy or radiotherapy.
Wood distillate (pyroligneous acid) boosts nutritional traits of potato tubers
Authors: Fedeli Riccardo , Vannini Andrea , Grattacaso Martina , Loppi Stefano 
Published: April 2023

AbstractPotato is the fourth most widely consumed staple food in the world. This study investigated the effectiveness of 0.2% wood distillate (WD), a biostimulant derived from the pyrolysis of waste plant biomass, in boosting the nutritional quality of potato tubers. The results showed that application of WD significantly increased the content of soluble sugars (sucrose +56.3%; glucose +44.9%; fructose +62.2%), starch (+35.1%) and total carbohydrates (+16.8%). Antioxidants (total antioxidant power, polyphenols, flavonoids) and most mineral elements (K, Mg, Ca, Na, Fe, Zn) were not affected. A lower content of Cu (−17.8%) and P (−24.5%) was found in WD‐treated potato.
Weak Instruments, Degree of Risk Aversion and Equity Premium: Evidence from Singapore, South Korea and Taiwan
Authors: Lai Liona , Tam Henry 
Published: April 2023

AbstractUsing data from Singapore, South Korea, and Taiwan, we estimate the coefficient of relative risk aversion (RRA) in the constant relative risk aversion utility specification of the consumption‐based capital asset pricing model. Conventional instrumental variables methods find that the coefficient of RRA is low but the inverse of it—the elasticity of intertemporal substitution in consumption—is also low. Such contradictory findings could be attributed to instruments being weak. Using weak‐instrument robust tests, we find from the equity market data that the coefficient of RRA is rather high, which could potentially explain the high equity premiums in these three East Asian economies.