bluuebunny/crossref_metadata_2025_split huggingface dataset:
Accuracy comparison of tooth volume and mesiodistal diameter measurements for sex dimorphism based on cone-beam computed tomography: a study for the northern Chinese population
Authors: Bu Wenqing , Ji Lingling , Han Mengqi , Wu Zixuan , Sultan Badr , Chen Teng , Tang Yu , Guo Yucheng , Wang Fei 
Published: February 2023

Abstract
Sex estimation based on teeth could help to narrow the scope for individual identification. According to the different teeth morphology among both genders, we plan to establish a sex estimation method for the northern Chinese population through mesiodistal diameter and teeth volume measurements and compare the accuracy of the two methods. In this study, measurements were taken from cone-beam computed tomography images collected from 142 males and 140 females aged 21–59 years. The mesiodistal diameter and volume of the left canines and the first molars in both upper and lower jaws were measured and analyzed for suitable coefficients. We selected 80% samples as the training set to set up the logistic regression formulas and 20% as the test set to obtain accuracy. The accuracy of sex estimation by mesiodistal diameter can reach 87.50%, and the volume is up to 78.57%. The measurement of mesiodistal diameter is less time-consuming. This work established and tested a method to estimate sex for the northern Chinese population. Results showed that sex estimation based on the mesiodistal diameter of teeth has higher accuracy than the method based on teeth volume.
FLInt: single shot safe harbor transgene integration via Fluorescent Landmark Interference
Authors: Malaiwong Nawaphat , Porta-de-la-Riva Montserrat , Krieg Michael 
Published: February 2023

Abstract
The stable incorporation of transgenes and recombinant DNA material into the host genome is a bottleneck in many bioengineering applications. Due to the low efficiency, identifying the transgenic animals is often a needle in the haystack. Thus, optimal conditions require efficient screening procedures, but also known and safe landing sites that do not interfere with host expression, low input material and strong expression from the new locus. Here, we leverage an existing library of ≈300 different loci coding for fluorescent markers that are distributed over all 6 chromosomes in Caenorhabditis elegans as safe harbors for versatile transgene integration sites using CRISPR/Cas9. We demonstrated that a single crRNA was sufficient for cleavage of the target region and integration of the transgene of interest, which can be easily followed by loss of the fluorescent marker. The same loci can also be used for extrachromosomal landing sites and as co-CRISPR markers without affecting body morphology or animal behavior. Thus, our method overcomes the uncertainty of transgene location during random mutagenesis, facilitates easy screening through fluorescence interference and can be used as co-CRISPR markers without further influence in phenotypes.
Multispecies polyploidization, chromosome shuffling, and genome extraction in Zea/Tripsacum hybrids
Authors: Iqbal Muhammad Zafar , Wen Xiaodong , Xu Lulu , Zhao Yanli , Li Jing , Jiang Weiming , Cheng Mingjun , Li Huaxiong , Li Yingzheng , Li Xiaofeng , He Ruyu , He Jianmei , Su Yuegui , Ali Asif , Peng Yan , Rong Tingzhao , Tang Qilin 
Published: February 2023

Abstract
By hybridization and special sexual reproduction, we sequentially aggregated Zea mays, Zea perennis, and Tripsacum dactyloides in an allohexaploid, backcrossed it with maize, derived self-fertile allotetraploids of maize and Z. perennis by natural genome extraction, extended their first six selfed generations, and finally constructed amphitetraploid maize using nascent allotetraploids as a genetic bridge. Transgenerational chromosome inheritance, subgenome stability, chromosome pairings and rearrangements, and their impacts on an organism's fitness were investigated by fertility phenotyping and molecular cytogenetic techniques genomic in situ hybridization (GISH) and fluorescence in situ hybridization (FISH). Results showed that diversified sexual reproductive methods produced highly differentiated progenies (2n = 35–84) with varying proportions of subgenomic chromosomes, of which one individual (2n = 54, MMMPT) overcame self-incompatibility barriers and produced a self-fertile nascent near-allotetraploid by preferentially eliminating Tripsacum chromosomes. Nascent near-allotetraploid progenies showed persistent chromosome changes, intergenomic translocations, and rDNA variations for at least up to the first six selfed generations; however, the mean chromosome number preferably maintained at the near-tetraploid level (2n = 40) with full integrity of 45S rDNA pairs, and a trend of decreasing variations by advancing generations with an average of 25.53, 14.14, and 0.37 for maize, Z. perennis, and T. dactyloides chromosomes, respectively. The mechanisms for three genome stabilities and karyotype evolution for formatting new polyploid species were discussed.
Seismic characterization of a fluid escape structure in the North Sea: the Scanner Pockmark complex area
Authors: Jedari-Eyvazi Farid , Bayrakci Gaye , Minshull Timothy A , Bull Jonathan M , Henstock Timothy J , Macdonald Calum , Robinson Adam H 
Published: February 2023

SUMMARYSubsurface fluid escape structures are geological features which are commonly observed in sedimentary basins worldwide. Their identification and description have implications for various subsurface fluid flow applications, such as assuring integrity of overburden rocks to geological CO2 storage sites. In this study, we applied 3-D first-arrival traveltime tomography to a densely sampled wide-azimuth and wide-angle ocean bottom seismometer (OBS) data set collected over the Scanner Pockmark complex, a site of active gas venting in the North Sea. Seismic reflection data show a chimney structure underlying the Scanner Pockmark. The objective of this study was to characterize this chimney as a representative fluid escape structure in the North Sea. An area of 6$\times $6 km2 down to a depth of 2 km below sea level was investigated using a regularized tomography algorithm. In total, 182 069 manually picked traveltimes from 24 OBS were used. Our final velocity model contains compressional wave velocity perturbations ranging from −125 to +110 ms−1 relative to its average 1-D model and compares favourably with a coincident seismic reflection data set. The tomographic velocity model reveals that the chimney as observed in seismic reflection data is part of a larger complex fluid escape structure, and discriminates the genuine chimney from seismic artefacts. We find that part of the seeping gas migrates from a deep source, accumulates beneath the Crenulate Reflector unconformity at ∼250 m below seafloor (mbsf) before reaching the porous sediments of the Ling Bank and Coal Pit formation at &lt;100 mbsf. In addition, the model shows that the venting gas at Scanner Pockmark is also being fed laterally through a narrow NW–SE shallow channel. Quantitative velocity analysis suggests a patchy gas saturation within the gas-charged sediments of the Ling Bank and the Coal Pit formations. Confined to the well-resolved regions, we estimate a base case average gas saturation of ∼9 per cent and in-situ gas volume of ∼1.64 $\times {10^6}\ {{\rm{m}}^3}$ across the Ling Bank and Coal Pit Fm. that can sustain the observed methane flux rate at the Scanner Pockmark for about 10 to 17 yr.
The telomere-to-telomere genome of Fragaria vesca reveals the genomic evolution of Fragaria and the origin of cultivated octoploid strawberry
Authors: Zhou Yuhan , Xiong Jinsong , Shu Ziqiang , Dong Chao , Gu Tingting , Sun Pengchuan , He Shuang , Jiang Mian , Xia Zhiqiang , Xue Jiayu , Khan Wasi Ullah , Chen Fei , Cheng Zong-Ming 
Published: February 2023

Abstract
Fragaria vesca, commonly known as wild or woodland strawberry, is the most widely distributed diploid Fragaria species and is native to Europe and Asia. Because of its small plant size, low heterozygosity, and relative ease of genetic transformation, F. vesca has been a model plant for fruit research since the publication of its Illumina-based genome in 2011. However, its genomic contribution to octoploid cultivated strawberry remains a long-standing question. Here, we de novo assembled and annotated a telomere-to-telomere, gap-free genome of F. vesca ‘Hawaii 4’, with all seven chromosomes assembled into single contigs, providing the highest completeness and assembly quality to date. The gap-free genome is 220 785 082 bp in length and encodes 36 173 protein-coding gene models, including 1153 newly annotated genes. All 14 telomeres and seven centromeres were annotated within the seven chromosomes. Among the three previously recognized wild diploid strawberry ancestors, F. vesca, F. iinumae, and F. viridis, phylogenomic analysis showed that F. vesca and F. viridis are the ancestors of the cultivated octoploid strawberry F. × ananassa, and F. vesca is its closest relative. Three subgenomes of F. × ananassa belong to the F. vesca group, and one is sister to F. viridis. We anticipate that this high-quality, telomere-to-telomere, gap-free F. vesca genome, combined with our phylogenomic inference of the origin of cultivated strawberry, will provide insight into the genomic evolution of Fragaria and facilitate strawberry genetics and molecular breeding.
Lodestar in the Time of Coronavirus? Interpreting International Obligations to Realise the Right to Health During the COVID-19 Pandemic
Authors: Bueno de Mesquita Judith , Lougarre Claire , Montel Lisa , Sekalala Sharifah 
Published: February 2023

ABSTRACT
While the right to health has gained significant momentum in international law over the past two years, there is little clarity on what it means for States to comply with this right in times of COVID-19. Taking Articles 2(1) and 12 of the International Covenant on Economic, Social and Cultural Rights as a starting point, our article follows an approach guided by the rules of treaty interpretation under the Vienna Convention on the Law of Treaties to suggest how right to health obligations to prevent, treat and control infectious diseases should be interpreted in relation to COVID-19, and how these obligations interact with general obligations of immediacy, progressive realisation, minimum core and international assistance and cooperation in this context. This article makes a novel contribution to clarifying the right to health during COVID-19, thus enhancing capacity for the oversight of this right; its incorporation in global health law; and the understanding of its corresponding obligations in future global health emergencies.
Population structure discovered in juveniles of Greenland halibut (Reinhardtius hippoglossoidesWalbaum, 1792)
Authors: Gíslason Davíð , Estévez-Barcia Daniel , Sveinsson Sæmundur , Hansen Agneta , Roy Denis , Treble Margaret , Boje Jesper , Vihtakari Mikko , Elvarsson Bjarki Þór , Hedges Kevin , Hallfredsson Elvar H , Johansen Torild 
Published: February 2023

AbstractUnderstanding the genetic differentiation among populations of most marine fish requires investigating the differences among spawning grounds. However, this can be challenging as spawning grounds for some species are not well known, or spawning fish are difficult to collect. An alternative is to collect juvenile fish in nursery habitats closely associated with potential spawning grounds. Greenland halibut is a deep-dwelling, commercially important species with at least two identified major offshore spawning grounds in the North Atlantic and weak genetic differentiation across the Atlantic. In this study, we sampled juveniles from three sites representing the Davis Strait spawning area in the northwest Atlantic and one site in the northeast Atlantic representing the primary spawning area along the western slope of the Barents Sea. We applied genotype by sequencing and discovered 90 genetic markers that could be used to assess genetic differentiation among the four sites. The northeast and northwest Atlantic showed major genetic differentiation, supporting the existence of the two primary spawning clusters. Additionally, we found genetic differentiation between the three northwest Atlantic samples implying the existence of more than one spawning area in the northwest.
Scattering resonances in unbounded transmission problems with sign-changing coefficient
Authors: Carvalho Camille , Moitier Zoïs 
Published: February 2023

AbstractIt is well known that classical optical cavities can exhibit localized phenomena associated with scattering resonances, leading to numerical instabilities in approximating the solution. This result can be established via the ‘quasimodes to resonances’ argument from the black box scattering framework. Those localized phenomena concentrate at the inner boundary of the cavity and are called whispering gallery modes. In this paper we investigate scattering resonances for unbounded transmission problems with sign-changing coefficient (corresponding to optical cavities with negative optical properties, e.g. made of metamaterials). Due to the change of sign of optical properties, previous results cannot be applied directly, and interface phenomena at the metamaterial-dielectric interface (such as the so-called surface plasmons) emerge. We establish the existence of scattering resonances for arbitrary two-dimensional smooth metamaterial cavities. The proof relies on an asymptotic characterization of the resonances, and shows that problems with sign-changing coefficient naturally fit the black box scattering framework. Our asymptotic analysis reveals that, depending on the metamaterial’s properties, scattering resonances situated close to the real axis are associated with surface plasmons. Examples for several metamaterial cavities are provided.
Electronic health record-based prediction models for in-hospital adverse drug event diagnosis or prognosis: a systematic review
Authors: Yasrebi-de Kom Izak A R , Dongelmans Dave A , de Keizer Nicolette F , Jager Kitty J , Schut Martijn C , Abu-Hanna Ameen , Klopotowska Joanna E 
Published: February 2023

Abstract

Objective
We conducted a systematic review to characterize and critically appraise developed prediction models based on structured electronic health record (EHR) data for adverse drug event (ADE) diagnosis and prognosis in adult hospitalized patients.

Materials and Methods
We searched the Embase and Medline databases (from January 1, 1999, to July 4, 2022) for articles utilizing structured EHR data to develop ADE prediction models for adult inpatients. For our systematic evidence synthesis and critical appraisal, we applied the Checklist for Critical Appraisal and Data Extraction for Systematic Reviews of Prediction Modelling Studies (CHARMS).

Results
Twenty-five articles were included. Studies often did not report crucial information such as patient characteristics or the method for handling missing data. In addition, studies frequently applied inappropriate methods, such as univariable screening for predictor selection. Furthermore, the majority of the studies utilized ADE labels that only described an adverse symptom while not assessing causality or utilizing a causal model. None of the models were externally validated.

Conclusions
Several challenges should be addressed before the models can be widely implemented, including the adherence to reporting standards and the adoption of best practice methods for model development and validation. In addition, we propose a reorientation of the ADE prediction modeling domain to include causality as a fundamental challenge that needs to be addressed in future studies, either through acquiring ADE labels via formal causality assessments or the usage of adverse event labels in combination with causal prediction modeling.
Safety risks and workflow implications associated with nursing-related free-text communication orders
Authors: Staes Catherine , Yusuf Saldi , Hambly Medalit , Phengphoo Saifon , Guo Jia-Wen 
Published: February 2023

Abstract

Objective
We evaluated nursing-related free-text communication orders to identify potential safety hazards and describe patterns and scope of care domains addressed that may reveal preventable workarounds and potential gaps in electronic health record (EHR) functionality.

Materials and Methods
A retrospective analysis of free-text EHR-based communication orders sent to or by nurses providing inpatient care at a major academic health system. Using built-in EHR tools and selection criteria, 13 193 orders were extracted, including 1373 unique orders. Using the Clinical Care Classification system standardized framework, we classified content by care domain and identified unique requests within each order. We reviewed each order for error-prone textual features based on standard patient safety guidance. We describe the distribution of domains, co-occurrence when 2 domains were present, and common patterns.

Results
The 1373 unique orders included a single request (65.3%), 2 requests related to 1 or 2 domains (19%), or 3 or more requests (15.7%). No orders included terms on the Joint Commission’s “Do Not Use” list. However, 13.6% of unique orders, and 16.7% of those related to medications, included error-prone symbols or abbreviations according to Institute for Safe Medication Practices guidance. Order content spanned 20 different care components but physical regulation, fluid volume, nutritional, safety, and medication were most frequently identified as single or co-occurring topics. Patterns were heterogenous.

Discussion
Free-text communication orders reveal workarounds, responses to upstream workarounds, and design constraints that should be further investigated. Remediation strategies are needed to reduce safety hazards and workflow impediments.

Conclusions
Analysis of free-text communication orders revealed opportunities for improvement.
#Coronavirus on TikTok: user engagement with misinformation as a potential threat to public health behavior
Authors: Baghdadi Jonathan D , Coffey K C , Belcher Rachael , Frisbie James , Hassan Naeemul , Sim Danielle , Malik Rena D 
Published: February 2023

AbstractCoronavirus disease (COVID)-related misinformation is prevalent online, including on social media. The purpose of this study was to explore factors associated with user engagement with COVID-related misinformation on the social media platform, TikTok. A sample of TikTok videos associated with the hashtag #coronavirus was downloaded on September 20, 2020. Misinformation was evaluated on a scale (low, medium, and high) using a codebook developed by experts in infectious diseases. Multivariable modeling was used to evaluate factors associated with number of views and presence of user comments indicating intention to change behavior. One hundred and sixty-six TikTok videos were identified and reviewed. Moderate misinformation was present in 36 (22%) videos viewed a median of 6.8 million times (interquartile range [IQR] 3.6–16 million), and high-level misinformation was present in 11 (7%) videos viewed a median of 9.4 million times (IQR 5.1–18 million). After controlling for characteristics and content, videos containing moderate misinformation were less likely to generate a user response indicating intended behavior change. By contrast, videos containing high-level misinformation were less likely to be viewed but demonstrated a nonsignificant trend towards higher engagement among viewers. COVID-related misinformation is less frequently viewed on TikTok but more likely to engage viewers. Public health authorities can combat misinformation on social media by posting informative content of their own.
Regulation of theMyD88gene in chicken spleen inflammation induced by stress
Authors: Ding Mengxia , Yu Yange , Zhu Zhaoyan , Tian Huihui , Guo Yujie , Zan Ruilong , Tian Yadong , Jiang Ruirui , Li Kui , Sun Guirong , Han Ruili , Li Donghua , Kang Xiangtao , Yan Fengbin 
Published: February 2023

AbstractIn order to investigate the regulatory role of the myeloid differentiation factor 88 (MyD88) gene in the stress inflammatory response to chicken spleen, the chicken stress model and macrophage (HD11) inflammation model were constructed in this study. Enzyme-linked immunosorbent assay and quantitative real-time PCR were used to investigate the effects of MyD88 on immune and inflammatory indicators. The results demonstrated that the levels of IgG, CD3+ and CD4+ in the serum of chickens in the beak trimming stress and heat stress groups decreased significantly compared to the control group without stress (P &lt; 0.05), and the inflammation-related indices IL-1β, TNF-α, IL-6 and NF-κB increased significantly (P &lt; 0.05). Stress up-regulated the expression levels of MyD88, IL-1β, NF-κB and TLR4 in the spleen, stimulated the release of inflammatory factors. Overexpression of MyD88 significantly up-regulated the expression levels of the inflammatory factors IL-1β, TNF-α, IL-8, NF-κB and TLR4 in HD11 cells (P &lt; 0.05). Co-treatment with lipopolysaccharide (LPS) further promoted the expression levels of the inflammatory cytokines in HD11 cells. Interference with the expression of MyD88 significantly reduced the expression level of inflammatory factors in HD11 cells (P &lt; 0.05) and had an antagonistic effect with LPS to alleviate the inflammatory reaction. In conclusion, the MyD88 gene has a pro-inflammatory effect and is highly expressed in the beak trimming and heat stress models in chicks, regulating the inflammatory response in poultry. It was involved in regulating the expression of immune-related genes in HD11 cells and had a synergistic effect with LPS.
A Systematic Review of ADHD Knowledge Measures and Their Psychometric Support
Authors: Golson Megan E , Brunson McClain Maryellen , Ha Jennifer , Stigler Renae , Bera Jac’lyn , Kieffer Kaelah E B , Gibson Abigail 
Published: February 2023

Abstract

Objective
Attention-deficit/hyperactivity disorder (ADHD) knowledge is associated with reduced stigma, earlier identification, and increased intervention access. Several ADHD knowledge measures have emerged. However, the psychometric quality of these measures varies wildly, and a review of the current psychometric support for ADHD knowledge measures is lacking.

Methods
The current study is a systematic review of ADHD knowledge measures for the reported psychometric support and the populations in which they are validated. The databases PsycINFO, ERIC, and PubMed were searched using PRISMA guidelines for peer-reviewed publications using a direct ADHD knowledge measure for original data collection. An ancestral search and the inclusion of dissertations were used to reduce potential publication bias. Included articles were coded for psychometric support, population of interest, and validation sample characteristics.

Results
A total of 163 articles were identified, including a total of 96 ADHD knowledge measures. The majority of measures (71.8%) did not include adequate psychometric evidence to constitute an evidence-based measure. Within that, approximately a third of ADHD knowledge measures were not accompanied by any psychometric support. Many measures are designed for and validated in only one population. Most studies did not report racial and ethnic validation sample composition; among those that did, there is a lack of diversity.

Conclusions
The lack of psychometric evidence for ADHD knowledge measures calls into question the current literature regarding ADHD knowledge, particularly related to racially and ethnically minoritized respondents with whom few measures have been validated. Implications for researchers and clinicians selecting an ADHD knowledge measure are discussed.