bluuebunny/crossref_metadata_2025_split huggingface dataset:
Reduction of elevated Gli3 does not alter the progression of autosomal recessive polycystic kidney disease
Authors: Russell Lauren G. , Kolatsi‐Joannou Maria , Wilson Laura , Chandler Jennifer C. , Tejedor Nuria Perretta , Stagg Georgie , Price Karen L. , Rowan Christopher J. , Crompton Tessa , Rosenblum Norman D. , Winyard Paul J. D. , Long David A. 
Published: January 2025

AbstractPolycystic kidney diseases (PKD) are genetic disorders which disrupt kidney architecture and function. Autosomal recessive PKD (ARPKD) is a rare form of PKD, caused by mutations in PKHD1, and clinically more severe than the more common autosomal dominant PKD (ADPKD). Prior studies have implicated Hedgehog (Hh) signaling in ADPKD, with increased levels of Hh components in experimental ADPKD and reduced cystogenesis following pharmacological Hh inhibition. In contrast, the role of the Hh pathway in ARPKD is poorly understood. We hypothesized that Hh pathway activity would be elevated during ARPKD pathogenesis, and its modulation may slow disease progression. We utilized Cpk mice which phenocopy ARPKD and generated a PKHD1‐mutant spheroid model in human collecting ducts. Significantly elevated levels of the Hh transcriptional effector Gli3 were found in Cpk mice, a finding replicated in PKHD1‐mutant spheroids. In Cpk mice, total GLI3 and GLI3 repressor protein levels were also increased. Reduction of increased Gli3 levels via heterozygous genetic deletion in Cpk mice did not affect cyst formation. Additionally, lowering GLI3 transcripts to wildtype levels did not influence PKHD1‐mutant spheroid size. Collectively, these data suggest attenuation of elevated Gli3 does not modulate murine and human models of ARPKD.
PENGARUH PENAMBAHAN LIMBAH BATU BATA MERAH BUKITTINGGI SEBAGAI FILLER PADA CAMPURAN AC-WC DENGAN PENGUJIAN MARSHALL
Authors: Misbah Misbah , Pratiwi JF Anggun , Arman Arman , Mulyati Mulyati , Rofifah Jihan 
Published: January 2025

Keberadaaan jalan raya sangat dibutuhkan dalam pengembangan suatu daerah. Permasalahan yang sering dihadapi dalam pembangunan jalan raya yakni muatan kendaraan berlebih dari yang diizinkan, pemeliharaan jalan yang kurang optimal serta pengaruh faktor campuran aspal, sehingga sering ditemukan jalan mengalami kerusakan sebelum umur rencana tercapai. Untuk meningkatkan kualitas konstruksi perkerasan jalan perlu dilakukan penelitian terhadap inovasi campuran yang digunakan atau memberikan bahan tambahan lain pada campuran konstruksi perkerasan tersebut, sehingga dihasilkan mutu campuran yang lebih baik. Jenis penelitian yang bisa dilakukan salah satunya adalah menggunakan metoda pembuatan benda uji dengan melakukan percobaan dilaboratorium dengan menganalisa pengaruh penambahan limbah batu bata merah Bukittinggi sebagai filler pada campuran AC-WC dengan pengujian Marshall. Dari hasil penelitian dengan pengujian Marshall yang telah dilakukan, dengan judul “Pengaruh Penambahan Limbah Batu Bata Merah Bukittinggi sebagai Filler pada campuran AC-WC dengan pengujian Marshall, didapat hasil pada penambahan variasi 1% filler limbah batu bata merah mengakibatkan nilai properties yaitu nilai : stabilitas, MQ, VMA, VFA memenuhi persyaratan spesifikasi Bina Marga 2018 revisi 2, sedangkan pada penambahan variasi 2% filler limbah batu bata merah mengakibatkan nilai properties yaitu nilai Flow dan VIM tidak memenuhi persyaratan spesifikasi Bina Marga 2018 rmevisi 2, hal ini menandakan meningkatnya deformasi pada lapis perkerasan saat diberikan beban. Maka, pada variasi penambahan 2% tidak dianjurkan, penggunaan limbah batu bata merah sebagai filler added pada campuran AC-WC yang dapat digunakan hanya sampai batas 1%.
Modeling of Multiphase Flow with the Wellbore in Gas-Condensate Reservoirs Under High Gas/Liquid Ratio Conditions and Field Application
Authors: Hong Jiajun , Wang Zhihua , Wang Cong , Zhang Jiuxiang , Liu Wenbo , Ling Kegang 
Published: January 2025

Summary
Due to the deeper burial depths of gas-condensate reservoirs, temperatures and pressures are much higher than in conventional reservoirs, resulting in the wellbore usually having a high gas/liquid ratio. The retrograde condensation phenomenon is often observed during the production process of such gas-condensate wellbores. Especially when heavy components are present in the gas-condensate wellbore, the appearance of wax particles occurs, and complex multiphase flow characteristics, with gas, liquid, and solid phases mixed flow, are formed. This leads to a serious threat to the safety and production within the wellbore in the gas-condensate reservoir under a high gas/liquid ratio, and even wellbore region blockage. The wax appearance and dynamic changes caused by retrograde condensation are fully considered in this study; through coupling the multiphase flow characteristics of a gas-condensate wellbore with the phase transition process, a mathematical model was established to predict multiphase flow in a gas-condensate wellbore. This model reveals the phase behaviors and wax appearance characteristics within the wellbore in the gas-condensate reservoir under a high gas/liquid ratio. As the wellbore depth decreases, phase changes occur in sequence with decreasing molecular weight, appearing in liquid and solid phases. The wax particles of the solid phase are mainly composed of C33* (C33–C40) and C25* (C25–C32), while the liquid phase formed by the retrograde condensation is primarily composed of C17* (C17–C24) and C9* (C9–C16). In addition, it establishes a general correlation between pressure drop, temperature drop, and wellbore depth in high gas/liquid ratio condensate wellbores, and the model’s error is controlled within a 5% range by validating with actual data. Finally, the model calculation results determined the flow pattern transition process as follows: From single-phase gas flow at the bottomhole to gas-liquid phases mist flow and gas-liquid-solid phases mist flow toward the wellhead, and in conjunction with the supercritical state of light hydrocarbons to provide an explanation for change characteristics of pressure drop at the transition boundary between different flow patterns.
Water-Alternating-Gas Performance in Highly Heterogeneous Carbonates: Simulation Study Applied to Cross Sections of Brazil Pre-Salt Fields
Authors: Kahrobaei Siavash , Lamine Sadok , Groenenboom Jeroen , van Batenburg Diederik 
Published: January 2025

Summary
A significant part of the production and associated reserves in the pre-salt cluster of the Santos Basin, offshore Brazil, comes from lacustrine carbonates that are deposited in successive horizontally extensive cyclothems that show strong vertical fine-scale variations with a Dykstra-Parsons (DP) coefficient larger than 0.95. The fields are developed with miscible gas injection (GI) and water-alternating-gas (WAG) injection almost from the start. In this paper, we build fine-scale cross-sectional models that honor the vertical small-scale permeability distribution observed in core measurements to quantify the interplay between pressure/volume/temperature, viscous, and gravity forces that are at play during gas and/or WAG injection.
For high heterogeneity levels representative of the cyclothem areas, the predicted gas/liquid ratio (GLR) and water cut exhibit cyclic behavior following the water injection (WI) and GI cycles. Similar behavior is observed in the field for the areas with high heterogeneity cyclothems. Under these conditions, the flow is essentially viscous-dominated as the strong vertical permeability contrasts are effectively resulting in low vertical permeability. For lower heterogeneity levels, the gravity forces become more dominant resulting in gas override with high GLR without clear cyclic behavior.
Furthermore, the effects of operating pressure, relative permeability hysteresis, gas trapping, and start time for the first gas slug on predicted oil recovery were evaluated. Including gas trapping and hysteresis improves the recovery but dampens the cyclic behavior. For the more heterogeneous models, the predictions show that it is beneficial to start the first gas cycle of the WAG injection early.
Representation of prime numbers on the complex plane
Authors: Csóka Levente 
Published: January 2025

Abstract

This study proposes a novel representation of prime numbers implying a quasiperiodic pattern within their distribution. Based on the result that any real number, and consequently, a prime number, can be represented in a complex plane using Euler’s notation; we investigated the quantities characterizing the sum of centres, which were observed as prime numbers. The numerical results indicate that prime numbers can be generated by rooting the real numbers, and their progression can be decomposed into periodic sequences. Within this framework, a harmonic prime function was identified, offering new insights into the structural intricacies of prime number sequences. The methodology employed advanced techniques to uncover the hidden periodicity underlying the quasiperiodic arithmetic progressions of primes, shedding light on the deeper patterns governing their distribution.
GNSS signal multi-frequency carrier phase asynchronous joint tracking
Authors: Wang Dun , Liu Peng , Peng Bo , Liu Tong , Chen Yaohui , Zhang Shuangna , Dong Qijia 
Published: January 2025

Abstract

As demonstrated in numerous studies, jointly tracking global navigation satellite system (GNSS) multi-frequency signals transmitted from the same satellite can improve tracking sensitivity and robustness. To facilitate the application of this joint tracking technique in contemporary GNSS receivers with asynchronous signal reception structures, we propose a synchronous-input asynchronous-update mechanism for multi-frequency carrier phase joint tracking. To elucidate the joint-tracking mechanisms, a linearized mathematical model of the joint tracking loop is constructed. We derive an equation to calculate the thermal noise jitter of the loop. Additionally, we examine the dynamic stress response characteristics of the loop and clarify the loop design requirements for eliminating the influence of inter-frequency biases. The theoretical results are validated using Monte Carlo simulations and preliminary field tests, with the B1C and B2a pilot signals from the medium earth orbit (MEO) satellite within the BeiDou satellite navigation system (BDS). Experimental results show that the carrier phase tracking threshold of the B1C and B2a signals can be reduced by approximately 1 dB and 3.5 dB, respectively.
WITHDRAWN: Configuration Paths for High-Quality Development of Listed Companies Basedon the TOE Framework: Evidence from China's high- and new-technologyenterprises
Authors: Qian Min , Yang Jiameng 
Published: January 2025

Abstract

The full text of this preprint has been withdrawn, as it was submitted in error. Therefore, the authors do not wish this work to be cited as a reference. Questions should be directed to the corresponding author.
WITHDRAWN: Mitigating Strength Loss in Mortar Due to Casting Delays: Quantified Impact of Additional Water and Cement
Authors: Mahzuz H. M.A. , Former Imran Hossain , Lizon Nasir Uddin Ahammed , Nury Ahmad Hasan 
Published: January 2025

Abstract

The full text of this preprint has been withdrawn by the authors due to author disagreement with the posting of the preprint. Therefore, the authors do not wish this work to be cited as a reference. Questions should be directed to the corresponding author.
A spatiotemporal atlas of human spermatogenesis
Authors: Lindskog Cecilia , Hikmet Feria , Méar Loren , Gustavsson Jonas , Miranda Gisele , Zhang Cheng , Katona Borbala , Schutten Rutger , von Feilitzen Kalle , Forsberg Mattias , Stukenborg Jan-Bernd , Uhlén Mathias 
Published: January 2025

Abstract

Human health depends on complex processes of intercellular interactions and single-cell type-specific functions. Here, we demonstrate that the integration of data from single-cell RNA sequencing (scRNA-seq) with fluorescent-based multiplex immunohistochemistry (mIHC) is a powerful strategy for large-scale spatiotemporal mapping of single cells in a tissue-specific context. We focused on the landscape of 12 distinct germ cell states in adult human testes and performed an in-depth characterization of ~ 500 proteins. Quantitative spatial localization data based on a custom-built image analysis pipeline allowed us to cluster proteins according to expression, forming the basis for functional analysis. Protein and mRNA expression dynamics showed multiple cases with low levels of co-expression in the same cell state, with proteins being expressed in later states in comparison to mRNA. This highlights the necessity of studying protein levels in single-cell mapping projects. The presented workflow holds promise for proteome-wide tissue studies in health and disease.
Ferroptosis in Neurons: A Bibliometric Analysis of Research Trends, Key Contributions, and Emerging Directions
Authors: Sun Shanshan , Wang Qiuxuan , Zhang Ziyi , Huang Jingjie , Huang Yue , Han Jingxian , Du Yuanhao , Zhang Xuezhu 
Published: January 2025

Abstract

Background
Neurons are the fundamental structural and functional units of the nervous system, serving as the core cells for information transmission and regulation. They are closely associated with various neurological diseases. Recent studies have shown significant advancements in research on ferroptosis in neurons; however, there has been a lack of bibliometric analysis in this field. This study aims to provide a comprehensive overview of the knowledge structure related to ferroptosis in neurons through bibliometric methods, identify current research trends and hotspots, and predict potential future research directions.
Methods
We conducted a search for publications related to ferroptosis in neurons from 2014 to 2024 in the Web of Science Core Collection (WoSCC) database. Bibliometric methods were employed to analyze authors, institutions, countries, journals, and references using VOSviewer, CiteSpace, and the R package "bibliometrix".
Results
This study included 685 articles from 50 countries, with China and the United States being the leading contributors. The number of publications related to ferroptosis in neurons has shown a year-on-year increase. The primary research institutions are Central South University, Harbin Medical University, and the University of Melbourne. Free Radical Biology and Medicine is the most popular journal in the field, while Cell has the highest citation count. A total of 4,673 authors contributed to the research, with David Devos and Ashley I. Bush having the highest number of publications, while Scott J. Dixon had the most co-citations. Keyword analysis revealed that the fundamental molecular mechanisms of ferroptosis and its application in neurological diseases are the primary research focuses in this field.
Conclusion
This study is the first comprehensive bibliometric analysis summarizing the trends and developments in ferroptosis research in neurons. The study outlines and predicts global research hotspots and trends, providing valuable references for scholars studying ferroptosis.
C-reactive protein dissociation contributes to choroidal neovascularization in vivo: implications for age-related macular degeneration
Authors: Hernandez Maria , Romero-Vazquez Sara , Recalde Sergio , Bezunartea Jaione , Moreno-Orduña Maite , Belza-Zuazu Idoia , Garcia-Layana Alfredo , Adan Alfredo , Fernandez-Robredo Patricia , Molins Blanca 
Published: January 2025

Abstract

Choroidal neovascularization (CNV) and inflammation processes play an important role in retinal disease development and C-reactive protein (CRP) has been shown to contribute to development diseases like Age-related macular degeneration (AMD). Our aim was to evaluate whether monomeric and pentameric CRP (pCRP, mCRP) isoforms contribute to CNV in vivo and to characterize the mechanism of CRP dissociation in-vivo and in vitro. Both CRP isoforms were intravitreally (IVT) or intravenously (IV) injected in mice, CNV was laser-induced, retinography and fluorescein angiography were performed to evaluate edema. Lectin, mCRP, F4/80 and C5b9 localization were assessed by immunofluorescence and visualized under a confocal microscope. Intensity of fluorescence of mCRP (IF mCRP) was also quantified. To confirm pCRP dissociation in ARPE-19 cells and mice, pCRP was coupled to a fluorochrome and IVT injected. A statistical increase in CNV areas was observed in pCRP IVT injected males (p<0.05) while a statistical decrease was shown in females (p<0.05). After IV injection, pCRP males showed an increase in CNV areas only vs. mCRP injected mice (p<0.05) and in females the injection of pCRP injected mice showed higher CNV areas vs. vehicle (p<0.05) and vs. mCRP injected mice (p<0.05). Retinal edema after IVT CRP injection was observed mainly in mCRP injected mice. In females there was an IF mCRP statistical decrease in pCRP IVT injected mice vs. vehicle and a statistical increase in pCRP IV injected mice vs. vehicle (p<0.05). Mice injected with IVT isoforms showed F4/80 positive cells and C5b-9 deposition around the CNV areas. mCRP labeling was observed in the intercellular space of the endothelial cells in the angiogenic area and detected in pCRP IVT injected animals, demonstrating the dissociation of pCRP into mCRP both in vitro and in vivo in proinflammatory microenvironments. In conclusion, CRP administration increased CNV areas and edema in the retina, suggesting that CRP is activated in the CNV inflammatory environment. Additionally, pCRP dissociates in vivo into mCRP close to CNV damaged tissue, hypothesizing that mCRP is the CRP conformation that contributes to the exacerbation of the CNV process.
Piloting An Oral Health Training Program for Diabetes Educators in Australia
Authors: George Ajesh , Kong Ariana , Poudel Prakash , Arora Amit , Lau Phyllis , Griffiths Rhonda , Masoe Angela , Sofronoff Susanne , Martin Rachel E , Akerman Lorena , Wong Jennifer , Uthurralt Natalia , Priede Andre , Wong Vincent , Forsyth Cathryn , Marwaha Parul , Kezhekkekara Shwetha , Calache Hanny 
Published: January 2025

Abstract

Aims: To develop and pilot a diabetes oral health training program to assess its effectiveness in improving oral health knowledge and confidence of diabetes educators (DEs), and the acceptability, feasibility, and sustainability of integrating the training into practice.
Methods: A sequential mixed-methods design followed the development of an online three-module training program, which integrated videos, a screening tool, and resources to support oral health referrals. A pre-post knowledge and confidence questionnaire was analysed using a paired-sample t-test. Qualitative semi-structured interviews were recorded, transcribed and analysed using inductive thematic analysis.
Results: Twenty-one DEs from the states of New South Wales and Victoria participated. There was an overall significant difference in the knowledge scores pre-test (mean = 23.24, SD 4.4) compared to post-test (mean = 29.67, SD 2.01), (t=-6.11), p<0.001), and across all confidence variables on discussing, screening and referring clients to oral health services. Most participants agreed with the trainings perceived acceptability, feasibility, and sustainability, although some recommendations were made to improve long-term sustainability.
Conclusions: The results showed that using online training modules and tailored resources to support clinical practice effectively improved oral health knowledge and confidence in DEs and was acceptable, feasible, and sustainable for integration into clinical practice.
The Soil Microbiome of the Caatinga Drylands in Brazil
Authors: Rech Elibio , Tacca Luísa , Lima Rayane , de Oliveira Marco , Pascoal Patrícia , Bambil Deborah , Rosinha Grácia , Signor Diana , Freire Marcelo 
Published: January 2025

Abstract

Drylands represent a significant part of the Earth's surface and include essential and vulnerable ecosystems for the global ecological balance. The Caatinga, with its unique biodiversity adapted to the extreme conditions of this semi-arid region, offers a valuable opportunity to expand our knowledge about these ecosystems. Here, this work reveals the high microbial diversity in the soil and rhizosphere of the Caatinga, with the roots presenting more specialized communities. Bacteria such as Bacilli, Alphaproteobacteria and Firmicutesexcelled in critical functions such as nutrient cycling. Interplant differences suggested the influence of root exudates. The metagenomic study of interactions between microorganisms in the rhizosphere of selected plants revealed microbial biodiversity and contributed to our understanding of nutrient cycling, plant growth and resistance to water stress. In addition, they demonstrate biotechnological potential to address global challenges such as desertification and food security.
Assessing Parental Knowledge, Attitudes, and Practices Towards Antibiotic Use in Children: A Study in a teaching and training Tertiary Care Hospital in Mogadishu, Somalia
Authors: İsse Suad Abdikarim , DOĞAN Ahmet , Çelik Ali kutta , Ali Tigad Abdisad , Wehlie Jabir Abdullahi , Sheikh Sowda Abdikarim , TURKAN Mucahit , Ali Bisharo Omar , Ahmed Qasim shirar , Ali Ahmed Mohamed , Isse salma Abdikarim , Hilowle Fartun Mohamed 
Published: January 2025

Abstract

Background
The inappropriate use of antibiotics in children remains a global concern, contributing to antibiotic resistance. This study aims to assess parents' knowledge, attitudes, and practices regarding antibiotic use in children at a tertiary care hospital in Mogadishu, Somalia.
Methods
A cross-sectional descriptive study was conducted at Mogadishu-Somalia-Turkey Recep Tayyip Erdoğan Training and Research Hospital with 325 parent participants.
Results
14.5% of parents knew antibiotics treat bacterial infections, while 35.1% believed antibiotics could treat viral infections. 64.3% thought antibiotics could be used for colds. 57.8% recognized penicillin as an antibiotic. 78.8% were aware of allergy risks, but 60.6% reported stopping antibiotics when their child felt better.
Conclusion
Significant gaps in parental knowledge and misconceptions about antibiotics were identified. Targeted educational programs are crucial to enhance awareness and promote responsible antibiotic use.
A Novel Learning Automata and Genetic Algorithm-based Energy Efficient and Reliable Data Delivery Mechanism in Wireless Sensor Networks
Authors: Gudla Sateesh , Siripurapu Sridhar 
Published: January 2025

Abstract

Wireless sensor networks (WSNs) pose many challenges- in spite of their crucial role in data transmission and reception covering wide range of applications. However, in adverse environmental conditions, wireless link instability prevents sensed data from reaching the sink node as well as leading to packet loss. In order to improve reliability of data delivery, retransmission and redundancy-based strategies can be employed. Nevertheless, these strategies lead to higher energy consumption with longer packet delivery delays. Therefore, ensuring energy efficiency and consistent delivery of data is of primary concern with WSNs. The proposed research work is predominantly focused on the route construction by selecting best resourceful nodes in terms of all the vital parameters of sensor nodes like residual energy, buffer space, distance & link quality (that leads to packet dropping). This work presents a routing mechanism for WSNs that leverages learning automata (LA) and genetic algorithm (GA). At first LA is hired to generate initial population. Further, GA is employed to establish energy efficient and reliable routes. Moreover, a novel fitness function is developed with due consideration of parameters like residual energy, buffer space, distance and link quality. Thus, this approach guarantees best possible parent chromosomes for the genetic operations. Simulation results depict that this algorithm condenses energy consumption, diminishes data delivery delays, decreases the number of data transmissions, while outspreading lifetime of network.