bluuebunny/crossref_metadata_2025_split huggingface dataset:
Awareness of Vocational and Technical Education Lecturers in Utilising the Emerging Technology Posed by Covid-19 Pandemic
Authors: Olatoye Adeolu Oludare , Salami Sarafadeen Segun , Olore Rasidat Iyabo , Adepoju Adeola Idiat 
Published: February 2023

The study was carried out to examine the awareness of vocational and technical eduation lecturers in utilizing the emerging technology posed by Covid-19 pandemic in Oyo State Colleges of Education. To achieve the objectives of the study, three research questions were developed and answered while two null hypotheses were tested at 0.05 level of significance. The study adopted survey research design. The population for the study consisted of 150 lecturers. Total enumeration sampling technique was used for this research work. The questionnaire was faced and content validated by two experts from the Department of Business Education and one expert from Industrial Technical Education Department, Tai Solarin University of Education, Ijebu-ode. Cronbach Alpha method was adopted to ensure reliability of the instrument with the coefficient of r=0.96. This shows that the instrument is highly reliable. The questionnaire was administered on 150 respondents and all were successfully retrieved. Mean and Standard deviation were used to answer data related to research questions while t-test and ANOVA analysis were used to test the null hypotheses at 0.05 level of significance. The findings of the study revealed that awareness level of the Vocational and Technical Education lecturers on the use of available technological tools for teaching and learning is moderately high. The extent of usage of modern technological tools for teaching and learning is low compare to their awareness. Challenges faced by Vocational and Technical Education lecturers in using modern technological tools is moderately high. The study revealed that there is significant relationship between male and female Vocational and Technical Education lecturers on the extent of lecturers` awareness on the use of modern technological tools. The study as well revealed that there is significant relationship between the respondents on the challenges faced by Vocational and Technical Education lecturers in using modern technological tools for teaching and learning with respect to their years of experience. Based on the findings, it was recommended among others that government through a relevant agencies like Tertiary Education Trust Fund (TETFund) should assist in providing some adequate modern technological tools for Colleges of Education, school management should provide modern technological tools for the smooth running of teaching and learning, Seminars and Conferences on the use of modern technological tools be attended at an interval.
On Sight, Technology, and Science Fiction: Transhumanist Visions in Contemporary Canadian Dystopia
Authors: Cuadrado Payeras Lidia María 
Published: February 2023

This article examines a number of practices of observation as represented in contemporary Canadian dystopias in light of technological developments as seen by transhumanist thought. It argues that the transhumanist scopic practices that underlie their science-fictional imaginaries are in fact dystopian, and, as such, it takes examples from dystopian literature to illustrate how the nature of sight and seeing in the techno- and image-mediated context presents dangerous pitfalls for subject formation, identity politics, and agency. The article distinguishes between “vision” as a body of ideas and “sight” as the actual ways of seeing that may be reciprocal and create bonds of affectivity or, in the case of the transhumanist predicament, be instead founded on watching as the one-sided commodifying alternative.
Dengue incidence and length of viremia by RT-PCR in a prospective observational community contact cluster study from 2005–2009 in Indonesia
Authors: Riswari Silvita Fitri , Velies Dyana Safitri , Lukman Nurhayati , Jaya Ungke Anton , Djauhari Hofiya , Ma’roef Chairin Nisa , Myint Khin Saw Aye , Widjaja Susana , der Ven Andre van , Alisjahbana Bachti , Mast Quirijn de , Kosasih Herman 
Published: February 2023

Background
Dengue has become a major global health threat since being recognized three centuries ago. Important gaps remain in understanding the transmission dynamics of dengue virus (DENV) infection. This study reports the results of a prospective observational cluster study that investigated the incidence of symptomatic and asymptomatic infections and length of viremia among close community contacts of hospitalized DENV-infected patients.

Methodology/principal findings
Between 2005 and 2009, dengue-confirmed cases (n = 97) admitted to Hasan Sadikin Hospital in Bandung, Indonesia, were enrolled as index cases. Subsequently, twenty close community contacts (n = 1928) living with and around the index cases were included and followed up for up to 14 days. Body temperature was measured daily; blood samples were collected every 3–4 days and when reported fever. DENV infection was confirmed using Reverse Transcriptase–Polymerase Chain Reaction (RT-PCR), IgM rapid test, and Enzyme-linked Immunosorbent Assay (ELISA). Among the 1928 community contacts, a total of 72 (3.7%) acute DENV infections were diagnosed, which equates to an incidence of 636 cases per 1,000 person-years (95% Confidence interval (CI) 588 to 687 cases per 1,000 person-years). Twenty-nine cases (40%) were symptomatic (22 dengue fever (DF) & 7 dengue hemorrhagic fever (DHF)), and 43 (60%) were asymptomatic. Primary and secondary DENV infections were detected in 18 (25%) and 54 (75%) subjects. Among the RT-PCR positives, viremia was observed as early as seven days before fever onset and converted to negative as late as seven days after the onset of fever.

Conclusions
DENV infections are common among close community contacts of hospitalized dengue patients. The high number of asymptomatic infections and the observation that viremia precedes the onset of fever for up to seven days highlight the importance of unrecognized dengue transmission and the need for improved transmission control.
A generalized framework for estimating snakebite underreporting using statistical models: A study in Colombia
Authors: Bravo-Vega Carlos , Renjifo-Ibañez Camila , Santos-Vega Mauricio , León Nuñez Leonardo Jose , Angarita-Sierra Teddy , Cordovez Juan Manuel 
Published: February 2023

Background
Snakebite envenoming is a neglected tropical disease affecting deprived populations, and its burden is underestimated in some regions where patients prefer using traditional medicine, case reporting systems are deficient, or health systems are inaccessible to at-risk populations. Thus, the development of strategies to optimize disease management is a major challenge. We propose a framework that can be used to estimate total snakebite incidence at a fine political scale.

Methodology/Principal findings
First, we generated fine-scale snakebite risk maps based on the distribution of venomous snakes in Colombia. We then used a generalized mixed-effect model that estimates total snakebite incidence based on risk maps, poverty, and travel time to the nearest medical center. Finally, we calibrated our model with snakebite data in Colombia from 2010 to 2019 using the Markov-chain-Monte-Carlo algorithm. Our results suggest that 10.19% of total snakebite cases (532.26 yearly envenomings) are not reported and these snakebite victims do not seek medical attention, and that populations in the Orinoco and Amazonian regions are the most at-risk and show the highest percentage of underreporting. We also found that variables such as precipitation of the driest month and mean temperature of the warmest quarter influences the suitability of environments for venomous snakes rather than absolute temperature or rainfall.

Conclusions/Significance
Our framework permits snakebite underreporting to be estimated using data on snakebite incidence and surveillance, presence locations for the most medically significant venomous snake species, and openly available information on population size, poverty, climate, land cover, roads, and the locations of medical centers. Thus, our algorithm could be used in other countries to estimate total snakebite incidence and improve disease management strategies; however, this framework does not serve as a replacement for a surveillance system, which should be made a priority in countries facing similar public health challenges.
Determinants of mortality among hospitalized patients with COVID-19 during first and second waves of the pandemic: A retrospective cohort study from an isolation center in Kano, Nigeria
Authors: Dayyab Farouq Muhammad , Bashir Hussain Abdullahi , Sulaiman Abdulwahab Kabir , Iliyasu Garba , Hamza Muhammad , Yakasai Ahmad Maifada , Nashabaru Ibrahim , Saidu Hadiza , Ahmad Bashir Garba , Dabo Bashir , Abubakar Aminu Yusuf , Idris Ibrahim Musa , Yahaya Abdulrauf Sani , Ado Mustapha , Abdurrahman Ibrahim Sabo , Usman Hafizu Musa , Bello Mohammed Kabiru , Jaafar Jaafar Suleiman , Abdullahi Anifowose , Alhassan Abubakar Muhammad , Ahmad Abdulmalik , Allen Alika Ehima , Ezekiel Medu Oghenekevwe , Umar Muhammad Abdullahi , Abdullahi Muhammad B. , Sulaiman Sahabi Kabir , Hussaini Tijjani , Umar Amina Abdullahi , Tsanyawa Aminu Ibrahim , Shuaibu Sabitu Y. , Kabo Nasir Alhassan , Muhammad Basheer Lawan , Yahaya Mohammed Nura , Bello Imam Wada , Rajab Ashiru , Daiyab Abdulhakim Muhammad , Kabara Aminu Faruk , Garko Muhammad Sule , Habib Abdulrazaq Garba 
Published: February 2023

BackgroundCoronavirus disease 2019 (COVID-19) has emerged as an important cause of morbidity and mortality worldwide.The aim of this study is to identify the clinical predictors of mortality among patients with COVID-19 pneumonia during first and second waves in a treatment center in northwestern Nigeria.MethodsThis was a retrospective cohort study of 195 patients hospitalized with COVID-19 between April 2020 to March 2021 at a designated COVID-19 isolation center in Kano State, Northwest Nigeria. Data were summarized using frequencies and percentages. Unadjusted odds ratios and 95% confidence intervals and p-values were obtained. To determine independent determinants of mortality, we performed a stepwise multivariate logistic regression model.ResultsOf 195 patients studied, 21(10.77%) patients died. Males comprised 158 (81.03%) of the study population. In the adjusted stepwise logistic regression analysis, age>64 years (OR = 9.476, 95% CI: 2.181–41.165), second wave of the pandemic (OR = 49.340, 95% CI:6.222–391.247), cardiac complications (OR = 24.984, 95% CI: 3.618–172.508), hypertension (OR = 5.831, 95% CI:1.413–24.065) and lowest systolic blood pressure while on admission greater than or equal to 90mmHg were independent predictors of mortality (OR = 0.111, 95%CI: 0.021–0.581).ConclusionStrategies targeted to prioritize needed care to patients with identified factors that predict mortality might improve patient outcome.
PENGUKURAN KUALITAS LAYANAN BERDASARKAN DIMENSI SERVICE QUALITY (SERVQUAL) DENGAN METODE ZONE OF TOLERANCE (ZOT) DAN KANO PADA PET WORLD
Authors: Rahmawati Dewi , Aulawi Hilmi , Kurniawati Rina 
Published: February 2023

Pet World merupakan salah satu jasa pelayanan kebutuhan hewan peliharaan yang berada di Kabupaten Garut, namun pemenuhan tingkat kepuasan pelanggan belum dapat dilakukan secara optimal. Tujuan dari penelitian ini adalah untuk mengetahui tingkat layanan berdasarkan pada dimensi kualitas yang diberikan terhadap kepuasan pelanggan serta menghasilkan usulan perbaikan untuk meningkatkan kualitas layanan pada Pet World. Penelitian ini menggunakan pendekatan Mix Method dimana data kualitatif dilakukan untuk menguji validitas dan reliabilitas. Sedangkan data kuantitatif digunakan untuk pengolahan metode Servqual untuk mengukur kepuasan pelanggan, metode ZOT untuk mengukur apakah pelayanan masih dalam batas toleransi, dan metode kano untuk mengukur prioritas.Hasil penelitian menunjukan bahwa atribut layanan yang tidak memuaskan atau atribut yang memiliki gap score negatif yaitu dua atribut pada dimensi tangibles dalam segi kenyamanan dan keamanan, satu atribut pada dimensi reliability pada segi ketepatan dalam produk serta satu atribut pada dimensi responsivenes pada segi akses informasi. Selain itu, metode Servqual, ZOT, dan Kano relevan dengan penelitian yang dilakukan karena pada metode ini mengukur kualitas layanan berdasarkan pada dimensi kualitasnya serta berorientasi pada kepuasan pelanggan. Abstract[Measurement of Service Quality Based on Service Quality (Servqual) Dimensions With Zone of Tolerance (ZOT) and Kano on Pet World] Pet World is one of the services for pet needs in Garut Regency, but the fulfillment of the customer satisfaction level has not been optimal. The purpose of this study was to know the level of service based on the dimensions of quality provided to customer satisfaction and to produce suggestions for improvements to improve service quality at Pet World. This study used a Mix Method approach. The Qualitative data was used to test the validity and reliability. The quantitative data was used for processing the Servqual method to measure customer satisfaction, the ZOT method to measure whether the service is still within tolerance limits, and the Kano method to measure priority. The results showed unsatisfactory service attributes or attributes with a negative gap score were two attributes on the tangibles dimension in terms of comfort and security, one on the reliability dimension in terms of product accuracy, and one on the responsiveness dimension in terms of access to information. In addition, the Servqual, ZOT, and Kano methods are relevant to the research conducted because this method measures service quality based on its dimensions and does orient to customer satisfaction.Keywords: Kano; Mix Method; service quality; zone of tolerance
New data on the recently described Brazilian Cerrado hotspot endemic Cerradomys akroai Bonvicino, Casado et Weksler, 2014 (Rodentia: Cricetidae)
Authors: Bezerra Alexandra M. R. , Weksler Marcelo 
Published: February 2023

Abstract

Cerradomys akroai is a recently described rodent species, known from two localities in the Brazilian Cerrado, in southeastern Tocantins state (type locality), and in southwestern Piauí state. Herein we add a third locality recorded from a specimen collected in central Tocantins state, in a “cerrado sensu stricto” habitat. Morphological and molecular analyses confirm the identification. The three localities now known for the species are restricted to northern Cerrado domain, and two are located in sites severely impacted by anthropogenic activities. Current conservation category of Cerradomys akroai is Data Deficient, and additional information on its distribution and biology is required.
Photoredox-catalyzed three-component difluorobenzylation of quinoxalin-2(1H)-ones with unactivated vinylarenes and BrCF2CO2Et/HCF2CO2H
Authors: Shen Lu , Yuan Jin-Wei , Zhang Bing , Song Sai-Yi , Yang Liang-Ru , Xiao Yong-Mei , Zhang Shou-Ren , Qu Ling-Bo 
Published: February 2023

Abstract
An environmentally friendly strategy for the photo-catalyzed three-component reaction between quinoxalin-2(1H)-ones, vinylarenes, with inexpensive and easily accessible ethyl bromodifluoroacetate/sodium difluoromethanesulfinate is described. This protocol exhibits mild conditions, high efficiency, and excellent functional group tolerance, providing a highly efficient approach for the synthesis of difluorobenzylated quinoxalin-2(1H)-ones by the formation of two carbon-carbon bonds. A radical mechanism is responsible for this three-component transformation.
Coupling between the Stereocilia of Rat Sensory Inner-Hair-Cell Hair Bundles Is Weak, Shaping Their Sensitivity to Stimulation
Authors: Scharr Alexandra L. , Ó Maoiléidigh Dáibhid , Ricci Anthony J. 
Published: February 2023

The hair bundle is the universal mechanosensory organelle of auditory, vestibular, and lateral-line systems. A bundle comprises mechanically coupled stereocilia, whose displacements in response to stimulation activate a receptor current. The similarity of stereociliary displacements within a bundle regulates fundamental properties of the receptor current like its speed, magnitude, and sensitivity. However, the dynamics of individual stereocilia from the mammalian cochlea in response to a known bundle stimulus has not been quantified. We developed a novel high-speed system, which dynamically stimulates and tracks individual inner-hair-cell stereocilia from male and female rats. Stimulating two to three of the tallest stereocilia within a bundle (nonuniform stimulation) caused dissimilar stereociliary displacements. Stereocilia farther from the stimulator moved less, but with little delay, implying that there is little slack in the system. Along the axis of mechanical sensitivity, stereocilium displacements peaked and reversed direction in response to a step stimulus. A viscoelastic model explained the observed displacement dynamics, which implies that coupling between the tallest stereocilia is effectively viscoelastic. Coupling elements between the tallest inner-hair-cell stereocilia were two to three times stronger than elements anchoring stereocilia to the surface of the cell but were 100–10,000 times weaker than those of a well-studied noncochlear hair bundle. Coupling was too weak to ensure that stereocilia move similarly in response to nonuniform stimulation at auditory frequencies. Our results imply that more uniform stimulation across the tallest stereocilia of an inner-hair-cell bundlein vivois required to ensure stereociliary displacement similarity, increasing the speed, sensitivity, and magnitude of the receptor current.SIGNIFICANCE STATEMENTGeneration of the receptor current of the hair cell is the first step in electrically encoding auditory information in the hearing organs of all vertebrates. The receptor current is shaped by mechanical coupling between stereocilia in the hair bundle of each hair cell. Here, we provide foundational information on the mechanical coupling between stereocilia of cochlear inner-hair cells. In contrast to other types of hair cell, coupling between inner-hair-cell stereocilia is weak, causing slower, smaller, and less sensitive receptor currents in response to stimulation of few, rather than many, stereocilia. Our results imply that inner-hair cells need many stereocilia to be stimulatedin vivoto ensure fast, large, and sensitive receptor currents.
The cryo‐EM structure of the CENP‐A nucleosome in complex with ggKNL2
Authors: Jiang Honghui , Ariyoshi Mariko , Hori Tetsuya , Watanabe Reito , Makino Fumiaki , Namba Keiichi , Fukagawa Tatsuo 
Published: February 2023

AbstractCentromere protein A (CENP‐A) nucleosomes containing the centromere‐specific histone H3 variant CENP‐A represent an epigenetic mark that specifies centromere position. The Mis18 complex is a licensing factor for new CENP‐A deposition via the CENP‐A chaperone, Holliday junction recognition protein (HJURP), on the centromere chromatin. Chicken KINETOCHORE NULL2 (KNL2) (ggKNL2), a Mis18 complex component, has a CENP‐C‐like motif, and our previous study suggested that ggKNL2 directly binds to the CENP‐A nucleosome to recruit HJURP/CENP‐A to the centromere. However, the molecular basis for CENP‐A nucleosome recognition by ggKNL2 has remained unclear. Here, we present the cryo‐EM structure of the chicken CENP‐A nucleosome in complex with a ggKNL2 fragment containing the CENP‐C‐like motif. Chicken KNL2 distinguishes between CENP‐A and histone H3 in the nucleosome using the CENP‐C‐like motif and its downstream region. Both the C‐terminal tail and the RG‐loop of CENP‐A are simultaneously recognized as CENP‐A characteristics. The CENP‐A nucleosome–ggKNL2 interaction is thus essential for KNL2 functions. Furthermore, our structural, biochemical, and cell biology data indicate that ggKNL2 changes its binding partner at the centromere during chicken cell cycle progression.
L‐arginine homeostasis governs adult neural stem cell activation by modulating energy metabolism in vivo
Authors: Xu Mingyue , Guo Ye , Wang Min , Luo Xing , Shen Xuning , Li Zhimin , Wang Lei , Guo Weixiang 
Published: February 2023

AbstractNeurogenesis in the developing and adult brain is intimately linked to remodeling of cellular metabolism. However, it is still unclear how distinct metabolic programs and energy sources govern neural stem cell (NSC) behavior and subsequent neuronal differentiation. Here, we found that adult mice lacking the mitochondrial urea metabolism enzyme, Arginase‐II (Arg‐II), exhibited NSC overactivation, thereby leading to accelerated NSC pool depletion and decreased hippocampal neurogenesis over time. Mechanistically, Arg‐II deficiency resulted in elevated L‐arginine levels and induction of a metabolic shift from glycolysis to oxidative phosphorylation (OXPHOS) caused by impaired attachment of hexokinase‐I to mitochondria. Notably, selective inhibition of OXPHOS ameliorated NSC overactivation and restored abnormal neurogenesis in Arg‐II deficient mice. Therefore, Arg‐II‐mediated intracellular L‐arginine homeostasis directly influences the metabolic fitness of neural stem cells that is essential to maintain neurogenesis with age.
Ubiquitylation of BBSome is required for ciliary assembly and signaling
Authors: Chiuso Francesco , delle Donne Rossella , Giamundo Giuliana , Rinaldi Laura , Borzacchiello Domenica , Moraca Federica , Intartaglia Daniela , Iannucci Rosa , Senatore Emanuela , Lignitto Luca , Garbi Corrado , Conflitti Paolo , Catalanotti Bruno , Conte Ivan , Feliciello Antonio 
Published: February 2023

AbstractBardet‐Biedl syndrome (BBS) is a ciliopathy characterized by retinal degeneration, obesity, renal abnormalities, postaxial polydactyly, and developmental defects. Genes mutated in BBS encode for components and regulators of the BBSome, an octameric complex that controls the trafficking of cargos and receptors within the primary cilium. Although both structure and function of the BBSome have been extensively studied, the impact of ubiquitin signaling on BBSome is largely unknown. We identify the E3 ubiquitin ligase PJA2 as a novel resident of the ciliary compartment and regulator of the BBSome. Upon GPCR‐cAMP stimulation, PJA2 ubiquitylates BBSome subunits. We demonstrate that ubiquitylation of BBS1 at lysine 143 increases the stability of the BBSome and promotes its binding to BBS3, an Arf‐like GTPase protein controlling the targeting of the BBSome to the ciliary membrane. Downregulation of PJA2 or expression of a ubiquitylation‐defective BBS1 mutant (BBS1K143R) affects the trafficking of G‐protein‐coupled receptors (GPCRs) and Shh‐dependent gene transcription. Expression of BBS1K143R in vivo impairs cilium formation, embryonic development, and photoreceptors' morphogenesis, thus recapitulating the BBS phenotype in the medaka fish model.