bluuebunny/crossref_metadata_2025_split huggingface dataset:
Barriers and Facilitators to the Referral of Patients With Cancer for Physiotherapy Rehabilitation at Uganda Cancer Institute (UCI).
Authors: Nareeba Denis , Ainembabazi Bonita , Asiimwe Derrick , Atuhaire Gift , Ochom Kenneth Pascal , Whitehouse Zillah , Arubaku Wilfred , Nuwahereza Amon , Kazibwe Herman , Niyonsenga Jean Damascene 
Published: January 2023

Abstract
Background: Cancer and its treatment are linked to a variety of physical complications. Physiotherapy is recommended as the treatment of choice for improving cancer patients' physical function and quality of life. All patients with cancer and rehabilitation needs at Uganda Cancer Institute (UCI) don’t receive physiotherapy services. There is little information available about the factors influencing health workers' decisions to refer patients with cancer for physiotherapy rehabilitation in underdeveloped countries, particularly Uganda. This study aimed to determine barriers and facilitators to the referral of patients with cancer for physiotherapy rehabilitation at UCI.
Methods: A qualitative descriptive phenomenological design was adopted. In-depth interviews were performed with 12 Uganda Cancer Institute (UCI) health professionals who were chosen using a convenience sampling approach. Thematic analysis was used to analyze the results. The UCI Research Ethical Committee granted ethical approval.
Results: Participants reported that facilitators to the referral of patients with cancer for physiotherapy rehabilitation included, but were not limited to; inter-professional communication, clinical characteristics and positive outcomes of physiotherapy. On the other hand, hindering factors included; a knowledge gap, understaffing of physiotherapists, advanced cancer complications, and many more.
Conclusion: Doctors and nurses at UCI appreciate the role of physiotherapy in cancer management. A limited knowledge on the assessment of patients with cancer for rehabilitation needs and limited physiotherapy staffing constrains the referral of patients with cancer for physiotherapy rehabilitation at UCI.
Multiple PMQR genes including the rare qepA detected in Ciprofloxacin non-susceptible Escherichia coli and Klebsiella isolated from children under five years at hospital discharge, Kenya.
Authors: Kariuki Kevin , Diakhate Mame Mareme , Musembi Susan , Tornberg-Belanger Stephanie N. , Rwigi Doreen , Mutuma Timothy , Mutuku Elizabeth , Tickell Kirkby D. , Soge Olusegun O. , Singa Benson O. , Walson Judd L. , Pavlinac Patricia B. , Kariuki Samuel 
Published: January 2023

Abstract
Background: The increasing spread of fluoroquinolone resistant enteric bacteria is a global public health concern. Children recently discharged from the hospital are at high risk of carriage of antimicrobial resistance (AMR) due to frequent exposure to antimicrobials during inpatient stays. This study aimed to determine the prevalence, correlates of ciprofloxacin non-susceptibility, and distribution of plasmid-mediated quinolone resistance (PMQR) genes in Escherichia coli (E. coli) and Klebsiella spp isolated from children under five years being discharged from two Kenyan Hospitals.
Methods: E. coli and Klebsiella spp were isolated from fecal samples from children discharged from hospital and subjected to antimicrobial susceptibility testing by disc diffusion and E-test. Ciprofloxacin non-susceptible isolates were screened for seven PMQR genes using multiplex PCR. Poisson regression was used to determine the association between carriage of ciprofloxacin non-susceptible isolates and patient characteristics.
Results: Of the 280 ciprofloxacin non-susceptible isolates: 188 E. coli and 92 Klebsiella spp isolates identified among 266 discharged children, 195 (68%) were ciprofloxacin-resistant (MIC ≥ 1µg/mL). Among these 195 isolates, 130 (67%) had high level ciprofloxacin minimum inhibitory concentrations (MICs) (≥32 µg/mL). Over 80% of the isolates had at least one PMQR gene identified: aac(6’)lb-cr (60%), qnrB (24%), oqxAB (22%), qnrS (16%), and qepA (6%), howeverqnrA was not identified in any isolates tested. Co-carriage of qnrB with acc(6’)-lb-cr was the most predominant accounting for 20% of all the isolates. Ceftriaxone use during hospital admission and the presence of ESBL production were significantly associated with the carriage of ciprofloxacin non-susceptible E. coli and Klebsiella spp.
Conclusion: Ciprofloxacin non-susceptibility is common among E. coli and Klebsiella spp isolated from hospital discharged children in Kenya. Carriage and co-carriage of PMQR, including the newly identified qepA gene, were frequently observed. These findings suggest that children leaving the hospital may serve as an important reservoir for transmission of resistant E. coli and Klebsiella spp to the community. Enhanced surveillance for AMR determinants is critical to inform interventions to control antimicrobial-resistant bacteria.
Optical Properties of hBN Quantum Dots for Ammonia Gas Detection
Authors: Shojaee Shahla , Karamdel Javad , Berahman Masoud , Ahmadi Mohammad T. 
Published: January 2023

Abstract
Detecting ammonia gas in low concentration with proper selectivity is crucial due to its harmful effects on human health and industry. Even low ammonia concentration can cause a rapid burning sensation in the eyes, nose, and throat. In addition, it can react with copper, silver, and other heavy metals. Hence detecting the low concentrations of ammonia gas with proper selectivity is essential. Hexagonal boron nitride quantum dots introduced new properties and potentials for engineering applications and gas-sensing devices. A small perturbation from gas molecules can drastically alter the band structure of quantum dots. These changes in sub-bands can easily detect using the optical absorption spectrum. Using density functional theory, we have explored the difference in the optical properties of hexagonal boron nitride quantum dots in the presence of ammonia gas molecules. Results show that different concentrations of ammonia can alter the maximum optical absorption to higher energies. In addition, a few peaks are obverse related to the transitions due to ammonia molecules in the system. The selectivity of the different gas molecules is also explored. The size of quantum dots increased from 32 to 168 atoms, and we realized that by increasing their size, similar properties could achieve. These results not only provide insights into the optical properties of hexagonal boron nitride quantum dots but also open the possibility of designing gas sensors based on such structures.
A novel m7G-related signature to predict prognosis and immune status in patients with hepatocellular carcinoma
Authors: Wang Zheng , Chen Fengzhe , Liu Changhong 
Published: January 2023

Abstract
Background
7-methylguanosine (m7G) is one of the most common RNA methylation modification, which is closely related to the development of many types of tumors. However, the role of m7G-related genes in hepatocellular carcinoma (HCC) remains unclear. We wanted to investigate the influence of m7G-related genes on the prognosis of HCC and establish a novel model to assess the potential prognostic application in HCC.
Methods
Firstly, we screened differentially expressed m7G-related genes in HCC using The Cancer Genome Atlas (TCGA) database. Then, a novel risk model according to the m7G-related genes for prognosis was built via univariate and multivariate regression. Furthermore, its reliability was verified by Kaplan-Meier method, ROC analysis, PCA, and t-SNE analyses. Functional enrichment, immune status, tumor mutation burden, immune checkpoints, patients response to chemotherapeutics were analyzed among the different risk groups. Eventually, the novel m7G-related prognostic signature was validated in external ICGC and human protein atlas database.
Results
27 differentially expressed m7G-related genes were screened, and EIF4E, GEMIN5, WDR4, NCBP2, NUDT5 were used to build a novel prognostic model. Individuals in the high risk category possessed remarkable poorer overall survival than their counterparts. We demonstrated that m7G-related risk model could be utilized as an independent prognostic marker for HCC patients and showed a good concordance with the prediction. We discovered that clinicopathological characteristics, immune cell infiltrations, immune function and tumor microenvironment differed dramatically between two risk groups. Additionally, high risk patients may possess a better response to immunotherapy and chemotherapeutic drug therapy. Finally, ICGC and immunohistochemical images confirmed our results.
Conclusions
This study furnished a novel forecast signature for predicting the prognosis of HCC patients and built a considerable foundation for future researches of the relationships between m7G-related genes and immunity in HCC.
Comparison of protective efficacy between a live attenuated and an inactivated porcine reproductive and respiratory syndrome vaccine.
Authors: He Yanliang , Li Zhenguang , Su Weiwei , Yue Dongmei , Zhan Caichao , Chen Feng , Yan Xijun , Zhu Guangqiang , Wu Hua 
Published: January 2023

Abstract
The objective of this study was to comparative evaluate the protective efficacy of live attenuated and inactivated porcine reproductive and respiratory syndrome vaccines. Fifty-five healthy piglets free of PRRSV antigen and antibody were randomly assigned into four groups. Piglets of group 1 (n = 20) were inoculated with PRRS live vaccine TJM-F92 strain (Live vaccine TJM-F92). Piglets of group 2 (n = 20) were inoculated with commercial PRRS inactivated vaccine (Inactivated vaccine). Piglets of group 3 (n = 10) were inoculated with PBS (Negative control). Group 4 (n = 5) remained as a not-immunized and not-challenged control (Mock group). And half of piglets from groups 1 and 2 were randomly selected and challenged with HP-PRRSV TJ strain and PRRSV NADC30-like strain respectively at 49 days post immunization (dpi). Our data showed the level of antibodies arose from live vaccine was significantly higher and faster than that from inactivated vaccine after immunization. Neither high fever nor signs of clinical disease were observed in piglets immunized with live vaccine, whereas piglets in inactivated vaccine group exhibited serious clinical symptoms, pathological lesions, and viremia load after HP-PRRSV TJ strain challenge. No obvious clinical signs, gross pathology lesions and viremia load were found in live vaccine group, while severe clinical signs, pathology change of lungs and high viremia load were recorded in inactivated vaccine group after NADC30-like strain challenge. The results indicated that PRRS live vaccine (TJM-F92 strain) could effectively prevent the infection of HP-PRRSV TJ strain and virulent NADC30-like strain. Immunization with PRRS inactivated vaccine could not effectively arouse immune system and provide strong immune cross-protection. Therefore, it is necessary to select suitable, widely application and effective PRRS vaccine for PRRS control according to the situation of pig farms.
Digital Light Processing 3D Printing for Microfluidic Chips with Enhanced-resolution via Dosing- and Zoning-controlled vat Photopolymerization
Authors: Wang Huanan , Luo Zhiming , Zhang Haoyue , Chen Runze , Li Hanting , Cheng Fang , Zhang Lijun , Liu Jia , Kong tiantian , Zhang Yang 
Published: January 2023

Abstract
Conventional manufacturing techniques to fabricate microfluidic chips like soft lithography and hot embossing processes show limitations including difficulty in preparing multiple-layered structures, cost- and labor-consuming fabrication process, and low productivity. Digital light processing (DLP) technology has recently emerged as a cost-efficient microfabrication approach for 3D printing of microfluidic chips, wherein, however, the fabrication resolution for microchannel is still limited to sub-100 microns at best. We hereby developed an innovative DLP printing strategy for high-resolution and scalable microchannel fabrication via dosing- and zoning-controlled vat photopolymerization (DZC-VPP). Specifically, we proposed a modified mathematical model to precisely predict the accumulated UV irradiance for resin photopolymerization, thereby providing guidance for the fabrication of microchannel with enhanced resolution. By fine-tuning the printing parameters including optical irradiance, exposure time, projection region and step distance, we can precisely tailor the penetration irradiance stemming from the photopolymerization of the neighboring resin layers, therefore avoiding channel blockage due to UV over-exposure or compromised bonding stability owing to insufficient resin curing. Remarkably, this strategy can enable scalable and biocompatible fabrication of microfluidic drop-makers that can be used for cell encapsulation. In general, the current DZC-VPP method can enable major advances in precise and scalable microchannel fabrication, and represents a significant step forward for widespread applications of the microfluidics-based techniques in biomedical fields.
Residual Cholesterol and Residual Inflammation Risk Associated with In-Stent Restenosis
Authors: Ke Bingbing , Shen Aidong , Qiu Hui , Li Weiping , Chen Hui , Li Hongwei 
Published: January 2023

Abstract
Background
Remnant cholesterol (remnant-C) and inflammation are considered risk factors for in-stent restenosis (ISR). We evaluate the combined impact of remnant-C and high-sensitivity C-reactive protein (hsCRP) on ISR.
Methods
A total of 1,503 patients were consecutively enrolled in this study. Baseline and follow-up remnant-C and hsCRP concentrations were obtained. The individual and combined effects of remnant-C and hsCRP on ISR were examined.
Results
The median (interquartile range) follow-up for all patients was 2.0 (0.9, 3.9) years, and 244 patients (16.2%) were diagnosed with ISR. Multivariate Cox analysis showed that the risk of ISR was higher in patients at the highest quartile of remnant-C (Q4 vs. Q1: adjusted hazard ratio HR 1.482, 95% confidence interval CI 1.013–2.168, p = 0.042, p for trend 0.020) and hsCRP (Q4 vs. Q1: adjusted HR 1.620, 95% CI 1.121–2.339, p = 0.010, p for trend 0.006). Moreover, the combination of high remnant-C and hsCRP produced a higher predictive value (adjusted HR: 1.904; 95% CI: 1.328–2.729; p < 0.001) than low remnant-C and hsCRP. In a subgroup analysis, significant associations were observed between elevated remnant-C and hsCRP concentrations and the risk of ISR (HR 3.071, 95% CI 1.415–6.663, p = 0.005) in patients with LDL-C levels ≤ 1.8 mmol/L.
Conclusions
Our results show that elevated remnant-C or hsCRP is associated with a high risk of ISR. The combined analysis of remnant-C and hsCRP could improve risk identification since high levels significantly increase the risk of ISR.
First study of the helminth community structure of Marmosa constantiae (Didelphimorphia, Didelphidae) in an ecotone area between Cerrado and Amazonia, state of Mato Grosso, Brazil.
Authors: Silva Beatriz Elise de Andrade , Cardoso Thiago dos Santos , Vilela Roberto do Val , Pacheco Richard de Campos , Rossi Rogério Vieira , de Mendonça Ravena Fernanda B , Júnior Arnaldo Maldonado , Gentile Rosana 
Published: January 2023

Abstract

Marmosa constantiae is a species of marsupial, restricted to the central portion of South America. In Brazil, it is restricted to the states of Acre, Amazonas, Mato Grosso, Paraná and Rondônia. However, there is no study of the helminth fauna or helminth community structure for this marsupial. The aims of this study were to describe the species composition and to analyse the structure of the helminthic community of M. constantiae at the infracommunity level in an area of the Amazon Arc in Sinop, north of the state of Mato Grosso, Brazil. Parasites were searched in 53 specimens of this marsupial, among which 44 were infected with at least one helminth species. Parasitic helminths were counted and identified. Nine species were collected: seven nematodes, one cestode and one acanthocephalan. The most abundant species were Gracilioxyuris agilisis, Travassostrongylus scheibelorum, Pterygodermatites sinopiensis and Subulura eliseae. These species were the only dominants in the component community. No significant differences were observed in the abundance and prevalence of helminths between male and female hosts. Host body size significantly influenced the helminth abundance in males. The pattern of community structure considering the infracommunities in this locality indicated more species replacement than species loss along the environmental gradient. This is the first study to analyse the helminth fauna and the helminth community structure of M. constantiae.
The health Care Management of farmers who use pesticides in Northeast Brazil. Use of pesticides and The Care Management
Authors: Silverio Astrid Merino , Cunha Maristela Casé Costa , Almeida Márlon Vinícius Gama 
Published: January 2023

Abstract
In Brazil, the health of communities that use pesticides is still neglected due to the lack of greater social understanding of damage to health and an insufficient care network. The aim of the research was to understand the actions of the health care management network of farmers who use pesticides, in municipalities in the Northeast, Petrolina (PE) and Juazeiro (BA), from the perspective of the health care network (RAS). This is a qualitative research, with a methodological framework based on the Grounded Theory in a constructivist way. 26 health professionals and RAS managers participated, at the municipal and regional levels in Petrolina (PE) and Juazeiro (BA). Two sample groups were composed for data collection, carried out through semi-structured interviews. The analysis resulted in six categories, which, when integrated, made up the theoretical model “Model of care management for riverside farmers”. The RAS must have teams trained in the care of riverside farmers who use pesticides in primary care, and specialized teams in secondary and tertiary care, who are a reference. The theoretical model formulated considers that the RAS, strengthened in terms of diversity of actions and services, will provide better care management for farmers.
Analysis of risk factors for an increase in the cervical sagittal vertical axis after cervical laminectomy with lateral mass screw fixation
Authors: Wu Hong-Jin , Wang Guo-Hua , Wan Wen-Tao , Bian Han-Ming , Zhang Yi-Ming , Di Ming-Yuan , Chen Chao , Zhao Dong , Yang Hai-Yun , Ma Xin-Long , Wang Zheng , Yang Qiang 
Published: January 2023

Abstract
Background: In recent studies on factors affecting quality-of-life (QOL) of patients after cervical laminectomy with fixation, cervical sagittal parameters were considered to have a strong association with the QOL.And among these parameters, the cervical sagittal vertical axis(cSVA) is regarded as the most important one. Therefore the purpose of this study is to analyze risk factors for an increase in the cSVA after cervical laminectomy with lateral mass screw fixation (LLMF).
Methods: A retrospective analysis of 128 patients who underwent LLMF from October 2018 to June 2021. The following parameters were measured: McGregor's slope (McGS), C2 slope (C2S), C2-7 cobb angle (CL), cSVA, and T1 slope (T1S). Two groups were divided according to whether ΔcSVA is positive or negative, including the negative ΔcSVA group (Group N) and the positive ΔcSVA group (Group P). The basic characteristics of patients, clinical outcomes, and imaging parameters of the two groups were compared.
Results: A total of 77 patients were included in this study (46 men and 31 women, a mean age of 61.44 ± 7.23 years, and a mean follow-up time of 18.60 ± 5.20 months). There were 28 patients in Group N and 49 in Group P. Patients in the two groups were similar in basic characteristics. C2S, cSVA, and NDI were significantly different postoperatively (p=0.002,0.001, and 0.031). Group P had larger changes of C2S, cSVA, and T1S than that of Group S (p=0.001,p＜0.001, and p=0.008). ΔcSVA was significantly correlated with ΔMCGS, ΔC2S, and ΔT1S (r=0.310,0.577, and 0.435). Multivariate linear analysis revealed that ΔcSVA was associated with the ΔC2S and ΔT1S (β=0.552 and 0.314). Postoperative C2S demonstrated a significant correlation with clinical outcomes.
Conclusion: A smaller reduction in T1S after LLMF would be a risk factor for an increase in cSVA. Postoperative C2S increases would be necessary to maintain the horizontal gaze and the head's center of gravity if postoperative cSVA increases excessively. Postoperative C2S can also be a good predictor of clinical outcomes.
Trust Aware Secured Energy Efficient Fuzzy Clustering based Protocol in Wireless Sensor Networks
Authors: Krantikumar B , Velusamy R Leela 
Published: January 2023

Abstract
Recent advancements in Wireless Sensor Networks (WSNs) have generated interest in the field of sensor tracking events. Environment monitoring is one of the most important applications where WSNs are used to guarantee animal conservation. WSNs are crucial in many real-time applications because of their topology, scale, and communication capabilities. Data privacy and integrity are the essential components that maximize network, security, trust and effectiveness. The fundamental problem with WSNs is the effective communication and efficient information exchange among several sensor nodes then extend the lifespan of the WSNs. Along with these issues, it is necessary to measure an optimal path with the highest degree of reliability. In this paper, a new fuzzy and secured clustering algorithm is proposed for improving the energy efficiency and security. In the proposed method, clustering is proved to be the best strategy for energy management of WSN metrics because it uses trust based fuzzy logic to find malicious nodes and selecting reliable path for data communication. The simulation results show that the proposed technique detects progress in relation to a variety of performance parameters. When compared to other various existing protocols, the proposed work reduces energy consumption while increasing network lifetime and security of communication.
In-Silico analysis of TAL1, TAL2 and LYL1 gene associated with T-ALL
Authors: Choudhury Shouhartha 
Published: January 2023

Abstract
Background: The primary tumours-specific TAL1 and TAL2 genes translocate to T-ALL. Precisely, the TAL2 (T-cell acute lymphocytic leukaemia 2) gene translocates to the chromosome breakpoint of t (7; 9) associated with the T-cell. The TAL2 genes transform in the TCR beta-chain identified in chromosome 7. TAL1 and TAL2 correspond to the LYL1 gene and represent a unique subgroup of bHLH TFs mediators of T-cell leukaemogenesis. Also, the magnitude of a subgroup of bHLH TFs (TAL1/TAL2 and LYL1) genes conserve in evolution. Those nuclear transcription factor genes control the malignant growth of lymphocytes.
Objective: The study aimed to investigate a subgroup of bHLH TFs of TAL1, TAL2, and LYL1 genes in the mammalian genomes. An analysis of the T-ALL-associated genes is mandatory to explore the molecular mechanisms involved in chromosomal breakpoint and translocation in particular organisms.
Methods: Analysis data summarized the number of TAL1, TAL1, and LYL1 genes and particular bHLH domains in the two different genomes. Observation data suggested the tumour-specific translocation of the TAL1, TAL2, and LYL1 genes in humans. Also, the conserved domain, motifs, phylogeny, gene expression, chromosome location and gene network analysis documented a subgroup of bHLH transcription factor genes associated with T-ALL.
Conclusions: The wealth of new reports supported unique genetic and phenotypic attributes of T-cell-leukaemogenesis. Some advanced reports and new approaches also promote conventional wisdom. So, this study concluded a subgroup of bHLH transcription factor genes linked with the progress of T-cell leukaemogenesis.