bluuebunny/crossref_metadata_2025_split huggingface dataset:
Stability‐indicating normal‐phase HPLC method development for separation and quantitative estimation of S‐enantiomer of lacosamide in pharmaceutical drug substance and tablet dosage form
Authors: Vadagam Niroja , Haridasyam Sharath Babu , Venkatanarayana Muvvala 
Published: June 2023

AbstractLacosamide (LA) is an antiepileptic medicine that is used to treat tonic–clonic seizures, partial‐onset seizures, mental problems, and pain. A simple, effective, and reliable normal‐phase liquid chromatographic technique was developed and validated to separate and estimate the enantiomer of (S‐enantiomer) LA in pharmaceutical drug substance and drug product. Normal‐phase LC was performed using USP L40 packing material (250 × 4.6 mm, 5 μm) and a mobile phase of n‐hexane and ethanol at 1.0 ml min−1. The detection wavelength, column temperature, and injection volume used were 210 nm, 25°C, and 20 μl, respectively. The enantiomers (LA and S‐enantiomer) were completely separated using a minimum resolution of 5.8 and accurately quantified without any interference in a 25‐min run time. Accuracy study for stereoselective and enantiomeric purity trials was conducted between 10 and 200%, with recovery values ranging from 99.4 to 103.1% and linear regression values >0.997. The stability‐indicating characteristics were assessed using forced degradation tests. The proposed normal‐phase HPLC technique is an alternate approach to the official monograph methods (USP and Ph.Eur.) of LA, and it was successfully used in the evaluation of release and stability samples for both tablet dosage forms and pharmaceutical substances.
Financial innovation and banks performance in developing countries: Evidence from Ghana
Authors: Nyamekye Kofi Afriyie , Okyere Gabriel Asare , Owusu‐Ansah Emmanuel De‐Graft Johnson , Damoah Obi Berko Obeng , Quarshie Joseph , Asenso‐Twum Josephine 
Published: June 2023

AbstractIncrease in the diffusion of information communication technology in Africa has resulted in the increase of technology‐based financial products and processes. The rollout of financially innovative products and processes is being undertaken on both the macro level (by regulators and government agencies) and the micro level (banking institutions). To determine whether or not this capital‐intensive investment is worthwhile or otherwise, the study collected firm‐level data on banks operating in Ghana, West Africa, to examine the relationship between financial innovation indicators and bank performance. Using a quantitative design, the study employs panel regression to analyse a panel data of 21 banks (both local and foreign‐owned). operating in Ghana between 2007 and 2015 The results show that financial innovation, proxied by two industrywide interventions, has a positive and significant impact on bank performance. It is recommended that deposit‐taking institutions in developing countries invest in financial innovative services to increase their performance. On the macro level, governments and banking industry regulators in Africa are encouraged to invest in creating and supporting the proliferation of technology‐based systems that will improve banking processes.
Androgen receptor knockdown enhances prostate cancer chemosensitivity by down‐regulating FEN1 through the ERK/ELK1 signalling pathway
Authors: Xie Weijie , Li Shulin , Guo Huan , Zhang Jiawei , Tu Menjiang , Wang Rui , Lin Bingling , Wu Yuqi , Wang Xiangwei 
Published: June 2023

AbstractPurposeFlap endonuclease 1 (FEN1) is highly upregulated in prostate cancer and promotes the growth of prostate cancer cells. Androgen receptor (AR) is the most critical determinant of the occurrence, progression, metastasis, and treatment of prostate cancer. However, the effect of FEN1 on docetaxel (DTX) sensitivity and the regulatory mechanisms of AR on FEN1 expression in prostate cancer need to be further studied.MethodsBioinformatics analyses were performed using data from the Cancer Genome Atlas and the Gene Expression Omnibus. Prostate cancer cell lines 22Rv1 and LNCaP were used. FEN1 siRNA, FEN1 overexpression plasmid, and AR siRNA were transfected into cells. Biomarker expression was evaluated by immunohistochemistry and Western blotting. Apoptosis and the cell cycle were explored using flow cytometry analysis. Luciferase reporter assay was performed to verify the target relationship. Xenograft assays were conducted using 22Rv1 cells to evaluate the in vivo conclusions.ResultsOverexpression of FEN1 inhibited cell apoptosis and cell cycle arrest in the S phase induced by DTX. AR knockdown enhanced DTX‐induced cell apoptosis and cell cycle arrest at the S phase in prostate cancer cells, which was attenuated by FEN1 overexpression. In vivo experiments showed that overexpression of FEN1 significantly increased tumour growth and weakened the inhibitory effect of DTX on prostate tumour growth, while AR knockdown enhance the sensitivity of DTX to prostate tumour. AR knockdown resulted in FEN1, pho‐ERK1/2, and pho‐ELK1 downregulation, and the luciferase reporter assay confirmed that ELK1 can regulate the transcription of FEN1.ConclusionCollectively, our studies demonstrate that AR knockdown improves the DTX sensitivity of prostate cancer cells by downregulating FEN1 through the ERK/ELK1 signalling pathway.
Prognostic value of Lynch syndrome, BRAFV600E, and RAS mutational status in dMMR/MSI‐H metastatic colorectal cancer in a pooled analysis of Dutch and French cohorts
Authors: Zwart Koen , van der Baan Frederieke H. , Cohen Romain , Aparicio Thomas , de la Fouchardiére Christelle , Lecomte Thierry , Punt Cornelis J. A. , Sefrioui David , Verheijden Rik J. , Vink Geraldine R. , Wensink G. Emerens , Zaanan Aziz , Koopman Miriam , Tougeron David , Roodhart Jeanine M. L. 
Published: June 2023

AbstractBackgroundCurrent knowledge on prognostic biomarkers (especially BRAFV600E/RAS mutations) in metastatic colorectal cancer (mCRC) is mainly based on mCRC patients with proficient mismatch repair (pMMR) tumors. It is uncertain whether these biomarkers have the same prognostic value in mCRC patients with deficient mismatch repair (dMMR) tumors.MethodsThis observational cohort study combined a population‐based Dutch cohort (2014–2019) and a large French multicenter cohort (2007–2017). All mCRC patients with a histologically proven dMMR tumor were included.ResultsIn our real‐world data cohort of 707 dMMR mCRC patients, 438 patients were treated with first‐line palliative systemic chemotherapy. Mean age of first‐line treated patients was 61.9 years, 49% were male, and 40% had Lynch syndrome. BRAFV600E mutation was present in 47% of tumors and 30% harbored a RAS mutation. Multivariable regression analysis on OS showed significant hazard rates (HR) for known prognostic factors as age and performance status, however showed no significance for Lynch syndrome (HR: 1.07, 95% CI: 0.66–1.72), BRAFV600E mutational status (HR: 1.02, 95% CI: 0.67–1.54), and RAS mutational status (HR: 1.01, 95% CI: 0.64–1.59), with similar results for PFS.ConclusionBRAFV600E and RAS mutational status are not associated with prognosis in dMMR mCRC patients, in contrast to pMMR mCRC patients. Lynch syndrome is also not an independent prognostic factor for survival. These findings underline that prognostic factors of patients with dMMR mCRC are different of those with pMMR, which could be taken into consideration when prognosis is used for clinical decision‐making in dMMR mCRC patients and underline the complex heterogeneity of mCRC.
A unique expression pattern of LAG3 distinct from that of other immune checkpoints in diffuse large B‐cell lymphoma
Authors: Lee Hyunjee , Yoon Sang Eun , Kim Seok Jin , Kim Won Seog , Cho Junhun 
Published: June 2023

AbstractBackgroundAlthough some patients with diffuse large B‐cell lymphoma (DLBCL) show a response to immunotherapy, there are still many who do not respond. This suggests that various immune checkpoints are complicatedly intertwined in the composition of the tumor microenvironment of DLBCL.Patients and MethodsTo comprehensively understand the expression of various immune checkpoint genes in DLBCL, we performed NanoString assay in 98 patients to investigate 579 genes. In addition, we performed immunohistochemistry for LAG‐3 and PD‐L1 to compare the results with expression in NanoString assay.ResultsAs a result of hierarchical clustering of NanoString assay, 98 DLBCLs were classified into three tumor immune microenvironment clusters. Most immune checkpoint genes showed the highest expression in cluster A and the lowest in cluster C. However, the expression of LAG3 was the highest in cluster C and the lowest in cluster A, showing an expression pattern opposite to that of other immune checkpoint genes. In Cluster A, the expression of genes related to T‐cell activity such as CD8A and GZMB was increased. In Cluster C, the expression of genes related to major histocompatibility complex molecules was the highest. Immunohistochemical stains showed modest agreement with the NanoString results but did not help clustering.ConclusionOur results show that the unique expression pattern of LAG3 in DLBCL contrasts with that of other immune checkpoints. We suggest that the combination of anti‐PD‐1/PD‐L1 and anti‐LAG‐3 blockades in the immunotherapy of DLBCL patients can have a synergistic effect, improving the immunotherapy efficacy and outcome in DLBCL patients.
Recurring nodular scleritis following inactivated vaccination for COVID‐19
Authors: Peyman Alireza , Pourazizi Mohsen , Dehghani Shakiba , Ghorbani Sarah 
Published: June 2023

Key Clinical MessageThough COVID‐19 vaccination saved many lives all around the world, it has had many adverse effects including ophthalmologic side effects. It is important to report such adverse effects to provide timely diagnosis and management.AbstractSince the COVID‐19 global outbreak, many types of vaccines have been introduced. These vaccines have been associated with some adverse effects including ocular manifestations. Herein, we report a case of a patient who developed nodular scleritis shortly after receiving the first and second doses of the inactivated COVID‐19 vaccine, Sinopharm.
Neonatal rhizomelic chondrodysplasia punctata type 2 caused by a novel homozygous variant in the GNPAT gene
Authors: Sayed Jamal , Gamal Ahmed , Theyab Abdulrahman , Algahtani Mohamed , Aldaadi Banan Bakheet 
Published: June 2023

Key Clinical MessageRhizomelic chondrodysplasia punctata (RCDP) is a rare disorder (~1 in 100,000 live births) of faulty plasmalogen biosynthesis and defective peroxisomal metabolism. RCDP type 2 is specifically caused by glyceronephosphate O‐acyltransferase (GNPAT) gene mutations and is inherited as an autosomal recessive trait. The disorder is characterized by skeletal abnormalities, distinctive facial features, intellectual disability, and respiratory distress. The case report describes a newborn baby with a dysmorphic facial appearance and skeletal abnormalities who was admitted to neonatal intensive care with respiratory distress. His parents were first cousins. The whole exome sequencing for this patient identified an interesting homozygous variant in the GNPAT gene [GNPAT (NM_014236.4):c.1602+1G>A (p.?), Chr1 (GRCh37):g.231408138G>A]. This case report aims to highlight the patient's clinical presentation with the variant and the whole exome sequencing, indicating the identification of a novel mutation in the GNPAT gene causing RCDP type 2.
A case of intradural lumbar disc herniation
Authors: Bhattarai Utsav , Gurung Pritam , Shrestha Janam , Dhakal Sudan , Acharya Samir , Pant Basant 
Published: June 2023

Key Clinical MessageMRI remains the best tool in the diagnosis of this disease entity however preoperative diagnosis remains a difficult task. A high degree of suspicion is raised when intraoperative findings and preoperative image description become incompatible.AbstractLumbar disc herniation into the dural space is a rare phenomenon of lumbar disc degeneration with an unclear remaining pathogenesis. Intraoperative ultrasonography and histopathological examination of resected specimen help in the diagnosis of intradural disc herniation. Prompt surgery is recommended due to the high incidence of cauda equina syndrome.
Bronchobiliary fistulae as a complication of untreated pulmonary hydatid cyst presenting with bilioptysis: A report of two cases
Authors: Mardani Parviz , Kamran Hooman , Khosravi Fateme , Shahriarirad Reza , Shahabinejad Pardis , Geramizadeh Bita , Soleimani Neda , Amirian Armin 
Published: June 2023

Key Clinical MessageWe reported two patients, a 45‐year‐old lady and a 48‐year‐old man, known cases of untreated liver and lung hydatid cysts complicated with bronchobiliary fistulae. Surgery was performed, and bronchobiliary fistulae were diagnosed intraoperatively. Lobectomy was done on the lobe, which was chronically infected. Symptoms resolved after surgery in both cases. Green‐colored sputum in a patient with a history of echinococcosis should raise the physician's attention to the probability of a connection between the bronchial tree and the biliary tract. Surgery in advanced cases is a suitable therapeutic option.
Robotic‐assisted percutaneous pelvis fixation: A case report
Authors: Smith Austin F. , Carlson Jon B. 
Published: June 2023

Key Clinical MessageIt may be possible to extend the use of the robotic arm to pelvic and acetabular surgery leading to safe, repeatable screw placement, and less radiation exposure for patients, surgeons and OR staff.AbstractIn this case, a novel, robotic‐assisted technique was used to place a sacroiliac screw in a patient with unstable injuries of the pelvic ring. Intraoperative and postoperative fluoroscopic, radiographic, and CT imaging demonstrated a safely positioned 6.5 mm cannulated screw without unplanned cortical violation or impingement on neurovascular structures. To our knowledge, this is the first such reported case using a robot widely available in the Americas or Europe.
Energy‐efficient Electrochemical Hydrogen Production Combined with Biomass Oxidation Using Polyoxometalate and Metal Salts
Authors: Xu Weizhuo , Zhou Bingjie , Wang Qing , Xu Guohao , Li Ning , Liu Wei , Zhang Z. Conrad 
Published: June 2023

AbstractBiomass oxidation in anode electrolyte solution is investigated for energy‐efficient hydrogen production. The redox of polyoxometalate (POM) as both biomass oxidation catalyst in the solution and charge carrier through regeneration at the graphite electrode promotes hydrogenation generation at the cathode, but suffers from a limited rate of biomass degradation due to the complex structure of solid lignocellulosic biomass. Herein, transition metal salts (CuCl2, CrCl3 and FeCl3) are studied as the synergistic catalysts with POM to promote the degradation of raw biomass. Notably, FeCl3 has the most significant effect in promoting the hydrolysis of cellulose by coordinating with the oxygen donor groups of carbohydrates and helps re‐oxidize reduced POM to maintain high oxidation ability of the solution. As a result, the faraday efficiency of hydrogen production at the cathode could reach 90 %. Coupling oxidation of biomass by contiguously recharged metal salts and POM with electrochemical hydrogen evolution, pure hydrogen source with improved economics is obtained at low energy consumption.
Radical Covalent Catalysis: Recent Advances and Future Developments
Authors: Xiao Wei , Wu Jie 
Published: June 2023

AbstractRadical covalent catalysis, namely radical catalysis via a radical catalyst addition and elimination process, has emerged in the past few decades. This review is aimed to summarize the recent advances of radical covalent catalysis by highlighting some outstanding challenges and confirming potential areas of improvement, which may offer inspiration for future studies.
Computational modelling of explosions caused by failed Li‐ion batteries
Authors: Simancas Nicolás Santiago Navarro , Méndez Juliana Puello , Vianna Sávio Souza Venâncio 
Published: June 2023

AbstractThe implementation rate of renewable energy sources such as lithium‐ion batteries has grown over the last decade. Consequently, the number of explosion occurrences associated with these batteries has also increased. Such events are due to a process called thermal runaway (TR). The flamelet combustion approach has been widely used to model premixed combustion. However, its applicability for modelling accidental explosions from lithium‐ion batteries remains limited. Moreover, the effects and contributions from stress, strain, and wrinkling on the flame front in gas mixtures from Li‐ion batteries are not fully understood. As far as computational modelling is concerned, the same effects require further investigation. The current research investigates the performance of the flamelet approach for modelling premixed combustion scenarios caused by the gases ejected by a fully charged lithium‐ion‐phosphate (LFP) battery. A new laminar burning velocity correlation is proposed based on experimental data to calculate overpressure, flame position, and flame velocity in a semi‐confined geometry. Promising results are presented resorted by good agreement with experimental data.
Outcomes and predictors of patients with moderate or severe functional mitral regurgitation and nonischemic dilated cardiomyopathy
Authors: Li Jiangtao , Wei Xiang 
Published: June 2023

AbstractBackgroundPatients with functional mitral regurgitation (FMR) and nonischemic dilated cardiomyopathy (DCM) are associated with high mortality.ObjectivesOur study aimed to compare the clinical outcomes between different treatment strategies and identify predictors associated with the adverse outcomes.MethodsA total of 112 patients with moderate or severe FMR and nonischaemic DCM were included in our study. The primary composite outcome was all‐cause death or unplanned hospitalization for heart failure. The secondary outcomes were individual components of the primary outcome and the cardiovascular death.ResultsIn this study, the primary composite outcome occurred in 26 patients (44.8%) in mitral valve repair (MVr) group and 37 patients (68.5%) in medical group (hazard ratio [HR], 0.28; 95% confidence interval [CI], 0.14–0.55; p < .001). The 1‐, 3‐, and 5‐year survival rates for patients with MVr were 96.6%, 91.8%, and 77.4%, respectively, which were significantly higher than that of medical group: 81.2%, 71.9%, and 65.1%, respectively (HR, 0.32; 95% CI, 0.12–0.87; p = .03). Left ventricular ejection fraction (LVEF) < 41.5% (p < .001) and atrial fibrillation (p = .02) were independently associated with the primary outcome. LVEF < 41.5% (p = .007), renal insufficiency (p = .003), and left ventricular end‐diastolic diameter > 66.5 mm (p < .001) were independently associated with heightened risk for all‐cause death.ConclusionCompared with medical therapy, MVr was associated with a better prognosis in patients with moderate or severe FMR and nonischemic DCM. We observed that LVEF < 41.5% was the only independent predictor of the primary outcome and all individual components of secondary outcomes.
Low‐cost Photoactive Hybrid Materials: From Green Synthesis to Multi‐technique Analytical Characterization
Authors: Conterosito Eleonora , Toson Valentina , Boccaleri Enrico , Antonioli Diego , Milanesio Marco , Palin Luca , Barolo Claudia , Barbero Nadia , Galliano Simone , Gianotti Valentina 
Published: June 2023

AbstractLow‐cost photoactive hybrid materials for light management, based on neutral organic molecules intercalated into saponite, were prepared by facile green methods and characterized. TPBI (2‐(2’‐tosylaminophenyl)benzimidazole) was chosen as an organic host dye, envisioning its application as a downconverter in silicon or dye‐sensitized photovoltaic modules. Mixed with a cationic surfactant, the neutral molecule was intercalated with a sort of “trojan horse” approach by an easy, almost solvent‐free method, thus limiting its aggregation. The obtained material was characterized by combining spectroscopic, diffractometric, and microscopic techniques. Moreover, the intercalation of the dye, the species present in the interlayer, their stability and mutual interactions were assessed by TGA‐GC‐MS. We obtained a material containing a fluorescent dye in the solid state and stabilized by intercalation and dispersion into saponite. At last, this material was used to prepare a photoactive polymer by melt blending, obtaining materials with the desired optical properties, with Stokes shifts larger than 100 nm and an emission yield above 50%.
LINC01116 affects patient survival differently and is dissimilarly expressed in ER+ and ER− breast cancer samples
Authors: Karimi Taheri Mohammadjavad , Ghanbari Sogol , Gholipour Akram , Givi Taraneh , Sadeghizadeh Majid 
Published: June 2023

AbstractBackgroundBreast cancer is the most commonly detected cancer and one of the leading causes of cancer mortality. Emerging evidence supports that aberrant expression of lncRNAs is correlated with tumor progression and various aspects of tumor development.AimThis study aimed to evaluate the expression pattern of LINC01116 in breast cancer tissues and investigate the impact of LINC01116 on patients' survival.Methods and ResultsMicroarray and qRT‐PCR data analysis were performed, and the KM‐plotter database was used in this study. In addition, the gain of function approach was performed to examine the effect of LINC01116 on breast cancer cells in‐vitro. The results exhibited that LINC01116 is meaningfully upregulated in the ER+ tumor specimens compared to the ER– ones. Also, relative to normal tissues, the expression of LINC01116 in ER+ and ER– tumor tissues significantly increased and decreased, respectively. ROC curve analysis revealed the power of LINC01116 in distinguishing ER+ from ER– samples. Additionally, the Kaplan‐Meier survival analysis showed that the LINC01116 expression positively correlates with survival probability in all as well as ER+ patients. However, this correlation was negative in ER– patients. Furthermore, our results showed that the overexpression of LINC01116 induces TGF‐β signaling in ER– cells (MDA‐MB‐231), and microarray data analysis revealed that LINC01116 is significantly upregulated in 17β‐Estradiol treated MCF7 cells.ConclusionIn conclusion, our results suggest that LINC01116 can be a potential biomarker in distinguishing ER+ and ER– tissues and has different effects on patients' survival based on ER status by affecting TGF‐β and ER signaling.