bluuebunny/crossref_metadata_2025_split huggingface dataset:
Связи зависимости от смартфона с личностными качествами и свойствами белорусов и россиян
Authors: Шейнов Виктор П. , Низовских Нина А. , Белых Татьяна В. , Девицын Антон С. 
Published: December 2022

Введение. Зависимость от смартфона − самая массовая из немедицинских зависимостей. Цель исследования – обнаружить общее и различия в связях зависимости от смартфона с личностными качествами и свойствами белорусских и российских мужчин и женщин (ассертивности, импульсивности, нарциссизма, незащищенности от манипуляций, зависимости от социальных сетей), сопоставив результаты, соответствующие этим группам респондентов.
Методы. Зависимость от смартфона диагностировалась опросником САС-16 (автор В.П. Шейнов), ассертивность – тестом ассертивности (В.П. Шейнов), незащищенность от манипуляций − опросником «Оценка степени незащищенности индивида от манипулятивных воздействий» (В.П. Шейнов), зависимость от социальных сетей − опросником ЗСС-15 (В.П. Шейнов, А.С. Девицын), импульсивность – методикой В. А. Лосенкова, нарциссизм − опросником Е. Кот. Результаты. В связях зависимости от смартфона с личностными качествами и свойствами в белорусской (n = 403) и российской (n = 361) выборках мужчин и женщин обнаружено больше общего, чем различий: зависимость от смартфона отрицательно связана с ассертивностью и положительно − с импульсивностью, потерей контроля над собой, страхом отказа использовать смартфон, эйфорией от его использования, зависимостью от социальных сетей. Вне зависимости от гендерной и территориальной принадлежности наиболее сильна связь между зависимостью от смартфона и потерей контроля над собой. Обнаружена отрицательная связь зависимости от смартфона с незащищенностью от манипуляций у белорусских женщин, тогда как у российских женщин данная связь является положительной. Выявлена отрицательная связь зависимости от смартфона с нарциссизмом у российских женщин при отсутствии ее у других групп респондентов.Обсуждение результатов. Полученные данные о связи зависимости от смартфона с личностными качествами и свойствами белорусов и россиян являются новыми, в целом они согласуются с результатами исследований в других социумах, при этом имеются некоторые отличия.
An Alternative Method for the Isolation and Identification of C-Terminal Peptide of Protein
Authors: Hsi Kuo-Liang , Nuwaysir Lydia M. , Dupont David R. 
Published: December 2022

Abstract:
A chemical approach for selectively isolating the C-terminal peptide from a protein is described. The protein to be analyzed is derivatized with glycinamide to protect carboxyl groups at both C-terminus and Asp/Glu side chains and subsequently fragmented with protease. The resulting peptides are treated with a Sepharose-based resin, EAH Sepharose 4B which binds all fragments except the C-terminal peptide, through the formation of amide bonds. The unbound C-terminal peptide is purified by HPLC and identified using Edman sequencing and mass spectrometry analyses.
Carboxyl-Terminal Sequencing: COOH-Amino Acid Activation with DCC or TBTU
Authors: Machado Olga L.T. , Ribeiro Patricia D , Alves Elias W. 
Published: December 2022

Abstract:
The present study explores conditions to activate the carboxyl group before the coupling of C-terrninal amino acid with Trimethylsilyl -isothiocyanate (TMS-ITC). Protected amino acids and different peptides were used as model and 1,3'-Dicyclohexylcarbodiimide (DCC) plus 1-Hydroxybenzotriazole (HOBt) and 2-(lH-Benzotriazol-l-y)- 1,1,3,3-tetramethyluronium tetrafluoroborate (TBTU) plus HOBt were used as carboxyl activators. The coupling reaction of activated AA with TMS-ITC was temperature dependent and the best yield (100 % of coupling) was obtained at 70°C, when DCC or TBTU both with HOBt were used as activating reagents.
Interaction of the Seed Lectin from Parkia Platycephala (Mimosoideae) with Carbohydrates and Complex Glycans
Authors: Ramos Marcio V. , Cavada Benildo S. , Bomfim Liezelotte R. , Debray Henri , Mazard Anne-Marie , Grangeiro Thalles B. , Rouge Pierre 
Published: December 2022

Abstract:
The carbohydrate-binding specificity of the Parkia platycephala (Mimosoideae) lectin was investigated by different techniques including hapten inhibition of haemagglutination, binding of lectin to immobilized glycoproteins and interaction in real time performed by surface plasmon resonance. The lectin belongs to the mannose/glucose-specific group and mannose and oligomannosides with al-3 and al-6 linkages are among its best inhibitors. The mannose/glucose-binding activity exhibited by this lectin, which belongs to a primitive group of Leguminosae, could be related to a defensive function against plant pathogens.
Purification and Partial Characterization of 5-Oxo-L-Prolinase from Pig Kidney
Authors: Wolf Sabine , Jiiger Martina , Weber Peter , Bangsow Thorsten 
Published: December 2022

Abstract:
5-0xo-L-prolinase (E.C. 3.5.2.9) was purified to homogeneity from porcine kidney. The molecular weight of one subunit was estimated to 135 kDa by SDS-PAGE. N-terrninal sequencing was not successful, leading to the conclusion that the enzyme is N-terminal blocked. Partial sequence data from a chymotryptic peptide showed strong similarities to the rat enzyme.
Zinc Promoted Simple Synthesis of Z-Arnino Acids Under Neutral Conditions
Authors: Suresh Babu Vommina V. , Gopi Hosahudya N. , Ananda Kuppanna 
Published: December 2022

Abstract:
The introduction of benzyloxylcarbonyl (Z) group into amino acids is described at neutral pH using benzyloxylcarbonyl chloride and activated zinc powder. The reaction is simple, fast and clean. Thus the Z-amino acids are prepared in good yield and purity
Design, Synthesis and DNA-Binding Activities of GCN4 Peptide Dimer Studied by Circular Dichroism Spectroscopy
Authors: Wang He , Lai Luhua , Miao Zhenwei , Xu Xiaojie 
Published: December 2022

Abstract:
Basic region-leucine zipper (bZIP) family of DNA-binding protein GCN4 truncate Ac-[L250) GCN4 (226--252)-GGC-OH and its two designed analogues were synthesized by solid phase method using Fmoc­ strategy. Circular dichroism spectroscopy measurements showed that the DNA-binding affinity with target AP- 1 site was reduced when Thr-236 in GCN4 basic region was replaced by valine or glutamine.
Purification and Crystallisation of a Novel Two-Domain Lectin from Scilla Campanulata
Authors: Reynolds Colin D. , Wright Lisa M. , Rizkallah Pierre J. , Allen Anthony K. , Peumans Willy J. , Van Damme Els , Donovan Michael J. 
Published: December 2022

Abstract:
In the last decade a number of single-domain mannose-binding lectins (11-14 kDa) have l:ieen characterised from different monocot plants and shown to belong to a superfamily of evolutionary related proteins. We have
isolated and crystallized a novel two-domain, multivalent lectin, with 26.2 kDa subunits, from Scilla campanulata bulbs. The native agglutinin crystallizes in space group C2 and 3.3A resolution data set has been recorded.
Synthesis, Structure and Neurotoxicity of the A Peptide of Alzheimer's Disease
Authors: Barrow Colin J. 
Published: December 2022

Abstract:
Amyloid formation and the A peptide are central to the neuropathology of Alzheimer's disease. This review discusses our work and related studies aimed at understanding the relationship between structure and neurotoxicity for the A peptide. These studies provide information on the importance of endogenous structural variants, metal coordination, conformation, and aggregation state, to the amyloidogenicity and neurotoxicity of the A peptide. Also discussed is improved methodology for the synthesis of this difficult peptide.
New Aspects of Angiotensin Receptor Regulation: Implications for Other Seven Transmembrane-Spanning Receptors
Authors: Thomas Walter G. 
Published: December 2022

Abstract:
Phosphorylation and internaliz.ation acutely regulate seven transmembrane-spanning, G protein-coupled receptors. Additional signalling and regulatory mechanisms have been recently proposed, including association of receptors with novel cytoplasmic proteins and a capacity of receptors to isomerize between multiple "active" states. This short review will introduce these mechanisms using receptors for the peptide hormone, angiotensin II, as a model for other G-protein coupled receptors.
Expression and Secretion of Biologically Active CMTI I in Saccharomyces cerevisiae
Authors: Leatherbarrow Robin J. , McNee John J. , Wang Fengshan 
Published: December 2022

Abstract:
A chemically synthesized gene encoding the squash seed inhibitor CMTI has been constructed and inserted into a yeast expression vector utilizing the a-factor promoter. Active inhibitor protein was found to be secreted into the medium. Mass spectrometry, kinetic analysis, protein sequencing and NMR were used to demonstrate that the protein had the correct sequence and was properly folded. This is the first time that expression of CMTI I in an active form has been described without the need for refolding in vitro.
Antiallergic Activity of Novel Hexapeptides Related to Immunoglobulin E
Authors: Kundu Bijoy , Shukla Manisha , Khare Sanjay K. , Gupta Prem P. , Singh Rashmi , Amarnath  
Published: December 2022

Abstract:
Eight novel hexapeptides corresponding to IgE Fe region (330-334) have been synthesized and evaluated for antiallergic activity by studying antiPCA as well as mast cell stabilizing activity. One of the peptides (CDRJ compound 95/220) exhibited high order of antiallergic activity.
α-Amylase Inhibitors from Cowpea Seeds
Authors: Melo Francislete R. , Sales Mauricio P. , Pereira Lucilene S. , Bloch Carlos , Franco Octavio L. , Ary Maria B. 
Published: December 2022

Abstract:
This work describes the first isolation and partial characterization of α-amylase inhibitors from cowpea (Vigna unguiculata) seeds. α-Amylase inhibitors were isolated using an affinity chromatography on Red Sepharose CL-6B. The bound Red Sepharose fraction was active against α-amylases from Bacillus sp., Aspergilus oryzae, V unguicu/ata seeds and also against α-amylases from Callosobruchus maculatus larvae.
Solid-Phase Synthesis of Hydrophobic Peptides on 1,6-Hexanediol Diacrylate Crosslinked Polystyrene Resin: Comparison with Merrifield Resin
Authors: Pillai V.N. Rajasekharan , Arunan C. 
Published: December 2022

Abstract:
A novel polymer support based on 1,6-hexanediol diacrylate and polystyrene was developed. The resin showed better solvation in solvents used for peptide synthesis than Merrifield resin. The synthetic utility of the new support has been illustrated with the synthesis of some hydrophobic peptides which have high affinity for aggregation. The yield and purity of the peptides were high compared to when Merrifield resin was used.
Purification and Crystallization of a Catechol 2,3-Dioxygenase PheB from Bacillus stearothermophilus BR219
Authors: Sugimoto Keisuke , Senda Toshiya , Masai Eiji , Kimbara Kazuhide , Fukuda Masao , Mitsui Yukio 
Published: December 2022

Abstract:
The thermostable catechol 2,3-dioxygenase from Bacillus stearothermophilus BR219 has been crystallized by vapor-diffusion method. The crystals were grown from a solution containing isopropanol, HEPES and magnesium chloride. The crystals belonged to the monoclinic system with a space group P21 and unit cell dimensions of a=69.4A, b=61.5A, c=l53.1A and β=92.6°.
Amino acid sequence and molecular modeling of a lipid transfer protein from the seeds of prosopis juliflora and its interaction with palmitic acid
Authors: Bloch Jr. Carlos , Negreiros Andre N ewton Monte , Cordeiro Artur Torres 
Published: December 2022

Abstract:
A basic, ninety two amino acid residue polypeptide, called CM4-3, was purified from seeds of Prosopis juliflora and sequenced. Sequence homology searches indicated that this protein is related to the nonspecific lipid transfer protein family (ns-LTP). A 3D model of CM4-3 was built base on the molecular coordinates of a maize ns-LTP and the obtained model revealed the presence of a highly conserved pocket formed by three a-helices capable of accommodating one molecule ofpalrnitic acid inside the CM4-3 structure.
The crystal structure of met-rantes: comparison with native rantes and aop-rantes
Authors: Hoover David M. , Shaw Jeffrey , Gryczynski Zygmunt , Proudfoot Amanda E. I. , Wells Tim , Lubkowski Jacek 
Published: December 2022

Abstract:
Met-RANTES is a modified version of the chemokine RANTES, which has an additional amino acid at the amino terminus. To compare Met-RANTES with the native protein as well as with another antagonist, AOP­ RANTES, we have solved the structure of Met-RANTES at 1.6 A by X-ray crystallography and investigated the dimerization of all three proteins by fluorescence anisotropy. The dimerization of AOP-RANTES shows an altered aggregation profile. The delineation of slight differences in binding sulfate anions by Met- and AOP­ RANTES might be linked to specific yet different interactions between these proteins and glycosaminoglycans.
A comparative study of the function of phospholipases a2 fromagkistrodon acutus
Authors: Zhou Yuancong , Liu Xiaolong , Xiong Ying , Wu Xiangfu 
Published: December 2022

Abstract:
cDNAs encoding acidic phospholipases A2I and acid A2II, basic phospholipase A2 and Lys49- phospholipase A2 from Agkistrodon acutus were inserted into bacterial expression vectors and effectively expressed in E.coli RRl. Except for Lys49-phospholipase A2, the other phospholipase A2s have high enzymatic activities. Acidic phospholipas_eA2I has an inhibiting effect on platelet aggregation. All phospholipase A2s except acidic phospholipase A2II have hemolytic activities. The roles of various amino acid residues in the enzymatic, hemolytic and platelet aggregation inhibiting activities of phospholipase A2 are discussed.
Ab initio study of the LOM-AKH-I peptide in gas­ phase environment
Authors: Zubrzycki Igor Z. 
Published: December 2022

Abstract:
Ab initio calculation (Hartree-Fock) using the minimal (STO-3G) basis set were performed on Lom­ AKH-I peptide, an insect neuropeptide belonging to the large adipokinetic hormones/red pigment concentrating hormones family (AKH/RPCH). The geometry optimized structure, energies and distribution of electrostatic charges were derived.
Effect of chiral guest residue on the conformation of short peptide containing the GLY-DPG-GLY segment: a cd investigation
Authors: Bhargava Kalpana , Rao R. Balaji 
Published: December 2022

Abstract:
The Gly-Dpg-Gly segment (Dpg = a ,a-Dipropylglycine) has already been shown to adopt a fully extended conformation in solution as well as in solid state. In this paper we investigate the effect of chiral residues and the terminal aromatic groups on extended conformation in solution with a Gly-Dpg-Gly backbone. CD studies on these peptides indicate that a chiral L-Leu residue at the C-terminus leads to a type I'/11' P-tum structure while a L-Leu residue at the N-terminus leads to a type,I/11P-turn. In addition we demonstrate the role of aromatic residues in the enhancement of CD intensities in the far UV region.
Divergences in homeodomains of hox genes and von baer's law
Authors: He Rong-Qiao , Yao Jun-Lan 
Published: December 2022

Abstract:
Divergences among homeodomains coded by Hox clusters of human beings have been analyzed with both methods of dynamic programming [I] and hydropathic mass [2). Homeodomains of the anterior genes (Hox A-1 to -4), including the paralogs of Hox B and Hox D, showed more distinguishable divergences than those of the posterior genes (from Hox A-5 to -13). The homeodomains grew exponentially more divergent in progression from the paralogue group I to 13 along a Hox complex. The divergence of homeodomains of Hox B-1 between C. elegans and human was small, but it became larger between those of the posterior genes. It appears that Hox genes should be involved in Von Baer's law.
Synthesis of alzheimer's B-amyloid peptide fragment (33-42) on a new chemically inert ps-bdodma support
Authors: Roice M. , Kumar K. S. , Pillai V. N. Rajasekharan 
Published: December 2022

Abstract:
The (33-42) fragment of Alzheimer's 13-amyloid peptide (Gly-Leu-Met-Val-Gly-Gly-Val-Val-Ile­ Ala) [1] was synthesised on a 2% 1,4-butanediol dimethacrylate cross-linked polystyrene (PS-BDODMA) support. The new polymer support was prepared by aqueous free-radical suspension polymerisation of styrene and 1,4-butanediol dimethacrylate. The peptide was synthesised.using Boe-amino acids and cleaved· from the resin by trifluoroacetic acid (TFA) in high purity and yield. The crude peptide was purified by HPLC and characterized by amino acid analysis and MALDI TOF MS.
An apolipoprotein e synthetic peptide targets lipid particles to lipoprotein receptors
Authors: Lokhov P.G. , Ipatova O.M. , Abakumova O.Yu. , Tsvetkova T.A. , Prozorovskii V.N. 
Published: December 2022

Abstract:
An apolipoprotein E 139-158 synthetic peptide was tested for targeting lipid particles to lipoprotein receptors. It was shown that this peptide increases lipid particle binding followed by receptor-mediated endocytosis by cells. In addition, the peptide mediates both acute clearance of lipoproteins followed by the decrease of cholesterol level in a rat blood and acute uptake of liposomes by the tissues which have a high level expression of lipoprotein receptors.
Single chain anti-idiotypic antibody mimics of the phytotoxin dothistromin
Authors: Jones William T. , Mett Vadim L. , Mett Valentina A. , Harvey Dawn , Reynolds Paul H.S. 
Published: December 2022

Abstract:
A single chain antibody mimic (MScFvlOE7) was constructed, using a phage display recombinant antibody system, from RNA isolated from the splenocytes of mice immunised with monoclonal antibody MAblOC12 which recognises the furan ring of DOTH [l]. MScFvlOE7 competed with DOTH for binding to MAblOC12 in two ELISA formats and .also reacted with the DOTH binding protein [2] extracted from mature Pinus radiata embryos and is a -type anti-idiotypic mimic of DOTH.
Temperature dependent michaelis-menten analysis of the bovine adenosine deaminase­ catalyzed deamination of adenosine
Authors: Britt B. Mark , Castro Christian 
Published: December 2022

Abstract:
Michaelis-Menten analysis of the bovine adenosine deaminase catalyzed deamination of adenosine from 10.0 - 40.0°C and at its pH optimum (pH=6.3) is reported. It is found that increases gradually and nearly linearly with T and exhibits a minimum between 10 and 15°C. The relative increase ofk... with Tis more pronounced and very linear. Values at the physiologic T of 38.3°C are: KM= 26±2 µM; k..,, = 99±4 s·1• The pseudosecond order rate constant (k../K has a maximal value of-3.8 µM·1s·1 in the physiological temperature range.
S-(1,2-dicarboxyethyl)glutatidone esters elevate glutatidone level in human hepatoma hepg2 cell
Authors: Tsuboi Seiji , Yasuda Makoto , Matsushima Satoshi 
Published: December 2022

Abstract:
The elevating effect of S-(1,2-dicarboxyethyl)glutathione (DCE-GS) esters on the glutathione (GSH) content was examined using human hepatocellular carcinoma cell line HepG2. The treatment with DCE-GS diester and triester (0.5 mM) increased the GSH level in HepG2 cells up to 145 and 175% over control level after 24h, respectively. DCE-GS diester and triester also elevated y-glutamylcysteine synthetase (y-GCS) activity in HepG2 cells up to 125 and 150% over the control level after 24h, respectively. However, this elevation of y-GCS did not occur on treatment with bis-(p-nitrophenyl)phosphate, non-specific esterase inhibitor. It is therefore concluded that DCE-GS diester and triester are transported into cells and hydrolyzed intracellularly to DCE-GS, and then DCE-GS produced promotes GSH synthesis due to the activation of y-GCS.
Crystalization and preliminary x-ray analysis of thermoactinomyces vulgaris R-47 α-amylase 1
Authors: Kondo Shin , Kaji Akira , Yuguchi Kazuyuki , Tonozuka Takashi , Sakano Yoshiyuki , Kamitori Shigehiro 
Published: December 2022

Abstract:
Crystals of Thermoactinomyces vulgaris R-47 a-amylase I, hydrolyzing cyclodextrins and pullulan, were grown by the vapor diffusion methods in two crystal forms with minor variations. The diffraction data were collected up to 2.5 A resolution and both crystals were belong to monoclinic system and a space group of C2 with cell dimensions ofa = 123.6 A, b = 51.7 A, and c = 109.1 A , 13 = 104.2° (crystal form 1) and a=
122.5 A, b = 55.0 A, and c = 108.7 A,13 = 103.8° (crystal form 2).
Crystallization and preliminary x-ray analysis of porcine aldehyde reductase in complex with coenzyme and quercetin
Authors: EI-Kabbani Ossama , Chung Roland P.-T. 
Published: December 2022

Abstract:
Aldehyde reductase, member of the aldo-keto reductase superfamily, catalyzes the reduction of a variety of aldehydes and ketones to their corresponding alcohols. Hexagonal crystals of porcine aldehyde reductase in complex with NADPH and the flavonoid inhibitor quercetin have been grown using the hanging-drop vapour­ diffusion method with ammonium sulfate as precipitant. The binding of quercetin into the active site of aldehyde reductase was revealed from a difference electron density map. This is the first crystallization report of an aldo-keto reductase in complex with a flavonoid.
Sensitive fluorogenic substrates for plasmepsin 2
Authors: Gulnik Sergei V. , Suvorov Leonid I. , Majer Pavel , Erickson John W. 
Published: December 2022

Abstract:
Structural Biochemistry Program, SAIC Frederick, National Cancer Institute-Frederick Cancer Research and Development Center, P.O Box B, Frederick, MD 21702-1201. Present Address: Tibotec Inc., 1330 Piccard Dr., Rockville, MD, 20850. 1Present Address: Gilford Pharmaceuticals Inc., 6611 Tributary Street, Baltimore, MD, 21224.

Plasmepsin 2 is a malarial aspartic proteinase that has been implicated in initial steps of hemoglobin degradation in parasite and thus represents an attractive antimalarial target. We designed and synthesized a number of quenched fluorogenic substrates and measured the kinetic constants of their cleavage by plasmepsin-2. High sensitivity and superior kinetic properties make the substrates suitable for different application including fluorescence plate reader.
Effect of a toxic protein isolated from zea mays seeds on the development and survival of the cowpea weevil, callosobruchus maculatus
Authors: Macedo Maria Ligia Rodrigues , Coelho Mirela Batista , Freire Maria das Gracas Machado , Machado Olga Lima Tavares , Marangoni Sergio , Novello Jose Carnillo 
Published: December 2022

Abstract:
A new toxic glycoprotein (Zeatoxin) has been isolated from Zea mays and sequenced. The toxin is a single polypeptide chain, with Mr of 10 kDa . Zeatoxin was not digested by a mixture of pepsin and papain or by rnidgut preparations of C. maculatus. A diet of artificial seeds (final concentration, 0.25%) was lethal to 50% of C. maculatus . These results show that Zeatoxin is a new toxic glycoprotein to C. maculatus. A search in the database indicated that the N-terrninal sequence show no homology to any other known protein.
A Novel Isoform of Osteoprotegerin Gene: Cloning and Expression and Its Hypocalcemic Effect in Mice
Authors: He Zhi-Yong , Yang Guan-Zhen , Chen Zhe-Yu , Li Biao , Zhang Wei-Jie , Wu Xiang-Fu 
Published: December 2022

Abstract:
A novel osteoprotegerin (OPG) isoform cDNA was cloned and expressed in the insect cell and yeast. No homodimeric OPG was found according to the SDS-PAGE analysis and western blot. The OPG isoform was 8 amino acid residues less than the reported hOPG. Genomic DNA of Chinese PBL and HEK 293 cells sequence analysis revealed that the mutation was not the result of RNA editing. The rhOPG with His-Tag at N­ terminal did not express the hypocalcemic effect in the normal mice.