bluuebunny/crossref_metadata_2025_split huggingface dataset:
KIN17 modulates cell proliferation and migration through the WNT/β-catenin signal pathways in non-small cell lung cancer
Authors: Peng Panli , Wei Wenjiang , Lin Shaohuan , Li Xvkai , Lv Junhong 
Published: April 2023

Abstract
Background
KIN17 DNA and RNA binding protein (KIN17) regulates the tumorigenesis of a variety of human cancers. Non-small cell lung cancer’s (NSCLC) molecular mechanisms for cancer metastasis and growth, however, are still unknown.
Methods
KIN17’s expression pattern as well as its prognostic value in LUAD patients was examined utilizing bioinformatics, and KIN17 co-expression network was enriched by the KEGG pathway and built via protein-protein interaction (PPI) network. Western blotting and Real-time quantitative PCR were employed to find Kin17 expression in NSCLC cells. Moreover, total amounts of EMT, WNT-related proteins, and β-catenin were also found using Western blotting. The expression of N‑cadherin, E-cadherin, and Vimentin was examined using immunohistochemical and immunofluorescent staining. The invasive and proliferative capacities of cancer cells were examined in vitro using wound healing, proliferation, cell cycle, transwell migration, and apoptosis. The impacts of KIN17 on the invasive and proliferative capacities in vivo were studied using the tumor xenograft model.
Results
On the basis of the TIMRE database, the mRNA expression of KIN was shown to be considerably higher in tumor tissues in contrast to adjacent normal tissues, and the protein expression of KIN was found to be considerably higher in LUAD from the National Cancer Institute. Then, in the LUAD from the TCGA database, patients with increased KIN17 expression had lowest disease free survival (DFS). The co-expression network of KIN17 enriched pathways were obtained by KEGG enrichment analysis and found to be linked to the tumorigenesis and development, including tight junction, WNT signaling pathway, regulation of actin cytoskeleton, and focal adhesion. In NSCLC cells, KIN17 was also upregulated in H1299 cell. With an increased expression of E-cadherin and a reduced expression of Vimentin and N-cadherin, KIN17 knockdown in H1299 cells greatly inhibited the ability of cells to invade, proliferate, and migrate. KIN17 knockdown likewise impaired WNT/β-catenin signal pathways, as indicated by downregulated expression of the WNT and β-catenin in vitro. Furthermore, KIN17 knockdown significantly reduced tumor renewal in NSCLC xenograft models.
Conclusions
By blocking the WNT/β-catenin pathway, KIN17 knockdown prevents EMT, NSCLC proliferation, and invasion.
Early-life exposure to a potent Aryl hydrocarbon receptor ligand results in persistent changes to the microbiota and host glucose homeostasis
Authors: Tian Yuan , Rimal Bipin , Bisanz Jordan E. , Gui Wei , Wolfe Trenton M. , Koo Imhoi , Murray Iain M. , Nettleford Shaneice K. , Yokoyama Shigetoshi , Dong Fangcong , Prabhu K. Sandeep , Turnbaugh Peter J. , Walk Seth T. , Perdew Gary H. , Patterson Andrew D. 
Published: April 2023

Abstract
Background Exposure to persistent organic pollutants (POPs) and gastrointestinal microbial disruption positively corelate with a predisposition to factors including obesity, metabolic syndrome, and type 2 diabetes; however, it is unclear if and how the microbiome contributes to this relationship.
Results Here, we show that early-life exposure to a potent aryl hydrocarbon receptor (AHR) agonist in mice resulted in persistent microbiota disruptions associated with impaired glucose homeostasis later in life. 2,3,7,8-tetrachlorodibenzofuran (TCDF)-exposed mice exhibited a profound disruption in the gut microbiome characterized by decreased abundances of Akkermansia muciniphila (A. muciniphila), decreased levels of cecal short chain fatty acids (SCFAs) and indole-3-lactic acid (ILA), and reduction of gut hormones GLP-1 and PYY. Importantly, microbial and metabolic phenotypes associated with early-life POP exposure were transferable to germ-free recipients in the absence of POP carry-over. Consistent with these in vivo studies, we reveal a direct, AHR-independent, POP-microbiota interaction that significantly affected the growth, physiology, gene expression, and metabolic activity of A. muciniphila, resulting in suppressed activity along the ILA pathway.
Conclusions These data point to a complex effect of POPs on the host and microbiota providing strong evidence that early-life, short-term, and self-limiting POP exposure can adversely impact the microbiome which persists into later life with associated health implications.
Analysis of red stains on building facades in Bahia – Brazil
Authors: ROCHA Tiago , GONÇALVES Vanessa , DUARTE Darlan , OMORE Abraham , MACHADO Marina , SILVA Francisco 
Published: April 2023

Abstract
The facades are essential constructive elements to the performance of buildings, because they influence in several characteristics such as the protection of the internal environment from external aggressive agents, such as temperature variation, winds, precipitations and humidity. Due to their high exposure to the environment they become more vulnerable structures to the appearance of pathological manifestations, which consequently contribute to an accelerated degradation process. The red stains are a type of pathological manifestation that occur in facades and portray the accumulation of microorganisms on the substrate of the seals that lead to the formation of biofilm in the red color. The objective of this work is to analyze the red stains on building facades in relation to the geographic orientation, vertical distribution of the stains on the surface of the facades, distance from the sea, and the surroundings of the buildings. For the development of the research, a data survey of nine buildings in the city of Salvador-Bahia and the Metropolitan Region was carried out. As main results, it was obtained that: the south-facing façades tend to be more affected by the red stains due to the higher humidity when compared to the others; there is a tendency in the concentration of stains in the upper region of the vertical plane of the façades; it was not found a correlation of the distance from the sea influencing the dissemination of the stains and buildings whose surroundings have a higher incidence of vegetation cover presented a higher proliferation of stains in their surroundings.
Development of a four species biofilm to mimic the upper gut microbial community
Authors: Marques Claudia , Tanzman Jacob , García-Rodriguez Alba , Kim Ashley , Tako Elad , Mahler Gretchen 
Published: April 2023

Abstract
The human gut microbiome is a dynamic ecosystem of microorganisms, influenced by numerous disparate factors including diet, age, and lifestyle. Investigating how changes in these variables affect the microbial gut community is limited due to the high variability found between individuals’ microbiota and limits inherent in sampling methodologies. Here we developed a microbial community with a defined species content - Lactocaseibacillus rhamnosus, Streptococcus salivarius, Enterococcus faecalis, and Bifidobacterium bifidum – mimicking the microbiota present in the small intestine. The biofilm community reached a steady state within 5–6 days of culture in a semi-batch system and once harvested and re-inoculated into a new reactor, the steady state was re-established within 24 hours. Biofilm development, microcolony structure, viability, and biomass were determined. Overall, this new “mock” community system can accurately mimic the small intestine microbiota and provide a platform to study community changes that occur due to environmental and chemical factors and lead to dysbiosis.
Environmental sustainability, healthcare workers and healthcare students: a literature review of attitudes and learning approaches
Authors: McCauley Pamela , Mathur Kavita , Cohen Nina , Henriques-Thompson Kedena , Gopinath Jyotsna , Miab Reza Kamali 
Published: April 2023

Abstract
Purpose: Today, increased attention has been placed on the healthcare industry and its workers in the wake of the global COVID-19 pandemic. Increases in facemask production, personal protective equipment, and the number of patients admitted into hospitals has resulted in a sharp rise in medical waste and environmental concerns. This has led to a greater interest by the research community to study the type of training and education that healthcare workers and students receive which could facilitate the implementation of sustainability activities during their work.
Design/Methodology: We have conducted a literature review (2010 to 2022) on healthcare worker knowledge and attitudes in environmental sustainability and reviewed educational initiatives –independent or academic- that have been proposed to implement training in sustainability for health professionals. For article retrieval we used standard and non-standard search engines and databases including Pubmed, Web of Science, medRxiv and Google Scholar.
Findings: The results indicate the important impact of worker attitudes towards sustainability and increased awareness of this issue affecting their work, but also limitations in knowledge and ability to participate in specific practical activities without organizational implementation.
Originality: This review provides insights into the multiple academic, professional and independent educational initiatives in sustainability that have been launched in the last decade in almost all areas of care and the limitations of healthcare workers to include these initiatives in their daily practices, due to multiple barriers for their actual implementation.
Listeria monocytogenes promotes breast cancer proliferation and enhances the survival rate of circulating breast cancer cells
Authors: Duan Yunqiang , Ma Fei , Guo Baoliang , Chen Ziang , Liu Yansong , Jiang Xiaohui , Rong Zhiyuan , Zou Yiyun , Zhang Hanyu , Wang Ting , Shang Yuhang , Feng Jianyuan , Hu Anbang , Cheng Weilun , Yu Tianshui , Li Mingcui , Li Yanling , Zhang Jiarui 
Published: April 2023

Abstract
Mammary gland microbiota is closely related to the progression of breast cancer (BC). Recent studies have shown that Listeria is differentially enriched in breast cancer and normal paracancerous breast tissue, and associated with epithelial-mesenchymal transition (EMT) in breast cancer. However, studies have rarely reported the relationship between Listeria monocytogenes (L. monocytogenes) enrichment and clinical parameters and possible mechanisms in breast cancer. In this study, we found that L. monocytogenes was highly enriched in the cytoplasm of cells, especially in tumor tissues. By analyzing the relationship between L. monocytogenesenrichment and the clinicopathological indicators and prognostic survival of patients, we found that L. monocytogenes is positively correlated with BC proliferation, metastasis, and poor recurrence-free survival (RFS) on patients. Moreover, we performed cell proliferation and invasion assays on breast cancer cells bearing L. monocytogenes, and proved that L. monocytogenes was able to promote the proliferation and invasion of breast cancer cells, their proliferation and invasion capacity was significantly reduced after antibiotic treatment. Mechanistically, L. monocytogenes was identified to increase the survival rate of circulating breast cancer cells by implementing the fluid shear stress model. In conclusion, our study demonstrated that L. monocytogenes could promote breast cancer development by increasing survival rate of circulating cancer cells.
Clinical evaluation of radiation-induced sinusitis by MRI-based scoring system in nasopharyngeal carcinoma patients
Authors: Zheng Wenya , Yan Tao , Liu Dongjiao , Chen Geng , Wen Yingjuan , Rao Xiuli , Wang Yizhe , Zheng Huijuan , Yang Jiahong , Peng Hua 
Published: April 2023

Abstract
Objective: To explore the application of magnetic resonance imaging (MRI) in the evaluation of radiation-induced sinusitis (RIS), MRI-based scoring system was used to evaluate the development regularity, characteristics and influencing factors of RIS in nasopharyngeal carcinoma (NPC) patients.
Patients and methods:A retrospective analysis was performed by collecting the clinical and MRI data of 346 NPC patients to analyze the characteristics and prognosis of RIS. The predictive model was constructed according to the influencing factors of RIS.
Results: (1) In the 2-year follow-up after radiotherapy (RT), there was significant change in L-M score in both groups of NPC patients (sinusitis before RT group: F=6.542, p=0.009 vs. non-sinusitis before RT group: F=11.702, p=0.000). After 6 months of RT, the L-M scores of the two groups tended to plateau (sinusitis before RT group: F=0.458, p=0.766 vs. non-sinusitis before RT group: F=0.500, p=0.736). (2) The prevalence of sinusitis in two groups of NPC patients (without or with sinusitis before RT) was 83% vs. 93%, 91% vs. 99%, 94% vs. 98% at 1, 6 and 24 months after RT, respectively. (3) In the patients without sinusitis before RT, the incidence of sinusitis in maxillary and anterior/posterior ethmoid, sphenoid and frontal sinuses was 87.1%, 90.0%/87.1%, 49.5%, 11.8% respectively, 1 month after RT. (4) A regression model was established according to the univariate and multivariate analysis of the factors related to RIS (smoking history: p=0.000, time after RT: p=0.008 and TNM staging: p=0.040).
Conclusion: (1) RIS is a common complication in NPC patients after RT. This disorder progressed within 6 months after RT, stabilized and persisted within 6 months to 2 years. There is a high incidence of maxillary sinus and ethmoid sinus inflammation in NPC patients after RT. (2) Smoking history, time after RT and TNM staging were significant independent risk factors for RIS. (3) The intervention of the risk factors in the model may prevent or reduce the occurrence of RIS in NPC patients.
Clinical and Metabolic Profile of Diabetic Patients With Supracondylar Amputation
Authors: Molina-Ormaza G. Nathaly , García-Vinces Pierina , Cedillo-Balcázar Jamil 
Published: April 2023

AbstractIntroduction:Micro and macrovascular complications of diabetes are a public health problem that affects the patient's quality of life and may require surgical amputation.Aim: To determine the clinical and metabolic profile of diabetic patients undergoing supracondylar amputationMethod:Observational, cross-sectional and descriptive study. Analyzing the medical records of 200 patients from a tertiary care hospital in EcuadorResults:60% of patients with amputation are over 65 years old, being more common the male gender; with 71% of the total cases with more than 10 years of diagnosis of Diabetes Mellitus, the comorbidity that predominates in them is arterial hypertension in 67.55% of cases. The predominant type of diabetic foot is the ischemic type. 70% with glycosylated hemoglobins over 7%. More than 50% of the cases had previously undergone minor amputations. metabolically speaking, 66% had BMIs >25, with low HDL cholesterol levels and elevated LDL cholesterol levels and elevated triacylglycerol levels.Diabeticpatients with supracondylar amputation have an altered metabolic profile (high cholesterol and triglycerides, poor glycemic control BMI >25). They have a long hospital stay due to the multiplecorbidities they present such as hypertension, stroke, diabetic retinopathy, diabetic nephropathy, ischemic heart disease; In addition to presenting anemia and leukocytosis, they are patients of care and difficult management both clinical and surgical and with high risk of morbidity and mortality.
The feasibility, acceptability, and preliminary effects of an acceptance and commitment therapy- based intervention for patients with chronic heart failure and their family caregivers: A pilot study
Authors: zhang Xuelin , Leung Doris Y.P. , Ho Grace WK , Mak Yim Wah 
Published: April 2023

Abstract
Background
Patients with chronic heart failure (CHF) often experience self-management challenges associated with psychological distress and impaired health-related quality of life (HRQOL) affecting both themselves and their family caregivers. Acceptance and commitment therapy (ACT) has shown positive effects on chronically ill patients or their caregivers, but has not been assessed in the context of CHF or applied in patient-caregiver dyads.
Aims
This study aimed to evaluate the feasibility, acceptability, and preliminary effects of a group ACT-based intervention for patients with CHF and their family caregivers.
Method
A single-group pretest and post-test study was conducted in a hospital in China. A total of five patient-caregiver dyads were recruited through convenience sampling. Data on recruitment, retention, intervention adherence, homework completion, and participant satisfaction were collected as an indication of feasibility and acceptability. The effects of the ACT intervention were assessed at two time-points (baseline and immediately after the intervention) in terms of self-reported HRQOL, anxiety, depression, relationship quality, perceived health status, psychological flexibility, self-compassion of patients and their family caregivers, patients' self-care behaviors, and caregiver burden.
Results
Recruitment, retention, intervention adherence, and homework completion rates were acceptable. Participants were generally satisfied with the program. The preliminary findings showed a trend of greater improvement over time for most outcome measures with small-to-medium within-group effect sizes.
Conclusions
The ACT-based intervention is generally feasible, acceptable, and effective in patients with CHF and their family caregivers. Some modification on the intervention protocol should be considered.
Rapid Procedure for the Testing of Nonnutritive Objects Found in Various Confectionary Products, Posing as a Possible Choking Hazard
Authors: Loechlt-Yoshioka Hans Y. 
Published: April 2023

Nonnutritive objects (toys) in confectionary food products can be a choking hazard for children under three years of age. A rapid method for the screening these types of toys is presented in this paper. It was found, that the use of a clear plastic cylinder is ideal for this rapid screening procedure of these products. By using this clear plastic cylinder, there was no need to measure each toy individually.https://doi.org/10.21423/jrs-v04n02p022 (DOI assigned 7/3/2019)
On-site performance evaluation of a 1,000-litre microbial fuel cell system using submergible multi-electrode modules with air-cathodes for sustainable municipal wastewater treatment and electricity generation
Authors: Heinrichmeier J. , Littfinski Tobias , Vasyukova Ekaterina , Steuernagel Leon , Wichern Marc 
Published: April 2023

Abstract
The reliability of a microbial fuel cell (MFC) system was tested on an industrial scale by operating a 1,000-L single-chamber system under real conditions at a municipal wastewater treatment plant (WWTP) over a 6-month period. Submergible multi-electrode modules with large-scale grid-segmented gas diffusion cathodes with activated carbon as a catalyst were used. Maximum power densities normalised to the cathode area were above 100 mW m−2Cat. Fluctuating chemical and physical wastewater characteristics of the influent had reversible effects on MFC performance in terms of energetic efficiency. Thereby, the composition of the chemical oxygen demand (COD) fractions changes only insignificantly and the concentration of readily biodegradable (SS) required for the enhanced biological phosphorus removal (EBPR) process or upstream denitrification was reduced by 41 ± 10 mg L−1 (37 ± 2% of inflow SS).
Effects of Trehalose Administration in Patients with
Mucopolysaccharidosis Type III
Authors: Mobini Moein , Radbakhsh Shabnam , Kubaski Francyne , Eshraghi Peyman , Vakili Saba , Vakili Rahim , Abbasifard Mitra , Jamialahmadi Tannaz , Rajabi Omid , Emami Seyed Ahmad , Tayarani-Najaran Zahra , Rizzo Manfredi , Eid Ali H. , Banach Maciej , Sahebkar Amirhossein 
Published: April 2023

Background and Aim:
Mucopolysaccharidosis type III (MPS III) is a rare autosomal recessive
lysosomal storage disease (LSD) caused by a deficiency of lysosomal enzymes required for the
catabolism of glycosaminoglycans (GAGs), mainly in the central nervous system. Trehalose has been
proposed as a potential therapeutic agent to attenuate neuropathology in MPS III. We conducted a single-
arm, open-label study to evaluate the efficacy of trehalose treatment in patients with MPS IIIA and
MPS IIIB.

Methods:
Five patients with MPS III were enrolled. Trehalose was administrated intravenously (15
g/week) for 12 weeks. Health-related quality of life and cognitive function, serum biomarkers, liver,
spleen, and lung imaging were assessed to evaluate trehalose efficacy at baseline and trial end (week 12).

Results:
TNO-AZL Preschool children Quality of Life (TAPQOL) scores increased in all patients, and
the mean scores for quality of life were increased after the intervention. Serum GAG levels were reduced
in all treated patients (however, the differences were not statistically significant). Alanine aminotransferase
(ALT) levels were reduced in all patients post-treatment (p=0.0039). The mean levels of aspartate
transaminase (AST) were also decreased after 12 weeks of treatment with Trehalose. Decreased serum
pro-oxidant-antioxidant balance and increased GPX activity were observed at the end of the study. Decreases
in mean splenic length were observed, whereas the liver volume did not change.

Conclusion:
Improvements in health-related quality of life and serum biomarkers (GAGs, liver aminotransferase
levels, antioxidant status), as well as liver and spleen size, were found following 3 months of
trehalose administration in patients with MPS IIIA and MPS IIIB.
Evaluation of Intracellular Metabolism of Methotrexate in Hepatocytes and Embryonic
Kidney Cells based on Folylpolyglutamate Synthetase and Gamma-Glutamyl
Hydrolase Expression
Authors: Yu Peng , Jiang Hanbing , Yang Jie , Hou Yucui , Zhang Ke , Ren Yi , Huang Jing , Li Huanhuan , Cai Tongji , Ouyang Zhonghua , Zhao Jia 
Published: April 2023

Background:
Methotrexate (MTX) is a common folic acid antagonist in clinical medicine, easily inducing
a common adverse side effect of liver and kidney injury. It has been found that the expression of Folylpolyglutamate
Synthetase (FPGS) and gamma-Glutamyl Hydrolase (GGH) may be closely related to that of related proteins to affect
the intracellular metabolism of MTX.

Objective:
The relationship between FPGS/GGH and MTXPGs accumulation in liver and kidney cells was explored
by adjusting the expression of FPGS and GGH in cells using UPLC-MS/MS quantitative technology.

Method:
Based on UPLC-MS/MS quantitative techniques, the relationship between MTXPGs accumulation and
FPGS/GGH in hepatocytes and embryonic kidney cells was explored by adjusting the expression of FPGS and GGH,
and the effect of FPGS/GGH on the intracellular toxicity of MTX was comprehensively analyzed.

Result:
The results showed that the difference in methotrexate polyglutamates (MTXPGs) accumulation in liver and
kidney cells was related to the difference in FPGS and GGH expression. The expression of FPGS interacted with that
of GGH. These results suggest that the protein abundance ratio of FPGS to GGH (FPGS/GGH) has more potential to
be used as a predictor of MTX efficacy than the FPGS or GGH single protein index. This can effectively avoid liver
and kidney damage caused by MTX and guides the rational use of drugs in MTX.

Conclusion:
The results prove that there is a positive correlation between the FPGS/GGH and the accumulation of
MTXPGS in liver and kidney cells. Summarily, the FPGS/GGH is expected to be a predictor for MTXPGs accumulation
and provides an effective method to evaluate the toxicity caused by MTX.