bluuebunny/crossref_metadata_2025_split huggingface dataset:
Delayed Death Due to Lightning: An Autopsy Case Report Highlighting Diagnostic and Medicolegal Issues
Authors: Khartade Harshwardhan Khushalrao , Shrivastava Shikha , Vashisht Jaya , Phukan Amborish , Palazhy Ponnambily P , Meshram Vikas P 
Published: May 2023

Abstract
The incidences of lightning strikes are not infrequent. The electric discharge of the lightning strike produces extreme heat and high amperage current, but fatality is reported in only about 10% to 30% of lightning cases. The spectrum of injuries due to lightning strikes can vary from no external injuries to typical external injuries. Merely the presence of superficial injuries due to lightning should not be considered sufficient to dismiss the case as non-serious. Rather clinicians should be wary of internal damage due to the effect of the lightning current as well as delayed complications of the lightning which can be fatal. Similarly, during an autopsy, the forensic pathologist should also investigate for the changes/damage in the internal organs due to the effect of lightning current. The present case reports the incidence of death due to delayed complications of a lightning strike which primary doctors initially overlooked. This case also discusses the histopathological changes in the internal organs due to lightning which can be helpful in the autopsy diagnosis of lightning, particularly in cases with no external or nonspecific injuries.
Amphipoda from depths exceeding 6,000 meters revisited 60 years on
Authors: Jamieson Alan J , Weston Johanna N J 
Published: May 2023

Abstract
In the 1950s, the Danish Galathea Expedition undertook one of the first and most comprehensive explorations of our ocean’s hadal zone, depths extending from 6,000 to nearly 11,000 m, and presented a rich collection of the diversity of Amphipoda. The subsequent papers, however, concluded that these established ‘nothing essentially new’ to the existing knowledge of amphipod biology. Since Dahl’s foundational paper in 1959, amphipods, primarily from the superfamilies Lysianassoidea and Alicelloidea, emerged as one of the best-sampled hadal fauna, as these mobile invertebrates are readily recovered by different sampling techniques. Importantly, amphipods have become the model taxon, helping us to unlock knowledge about life in the hadal zone. In this review, we collate the knowledge gained since the Galathea Expedition and summarise the current understanding of how amphipods that appear during hadal exploration survive the trench environments. We discuss population structures across depth, inter-trench distribution and connectivity, applications in hadal microbiology, and, critically, how the hadal zone is being impacted by anthropogenic activity.
Forecasting Value-at-Risk Using Deep Neural Network Quantile Regression
Authors: Chronopoulos Ilias , Raftapostolos Aristeidis , Kapetanios George 
Published: May 2023

Abstract
In this article, we use a deep quantile estimator, based on neural networks and their universal approximation property to examine a non-linear association between the conditional quantiles of a dependent variable and predictors. This methodology is versatile and allows both the use of different penalty functions, as well as high dimensional covariates. We present a Monte Carlo exercise where we examine the finite sample properties of the deep quantile estimator and show that it delivers good finite sample performance. We use the deep quantile estimator to forecast value-at-risk and find significant gains over linear quantile regression alternatives and other models, which are supported by various testing schemes. Further, we consider also an alternative architecture that allows the use of mixed frequency data in neural networks. This article also contributes to the interpretability of neural network output by making comparisons between the commonly used Shapley Additive Explanation values and an alternative method based on partial derivatives.
Diagnostic value of dual-fluorescence staining in bacterial vaginosis
Authors: Li Cui , Duan Zhongliang , Zhang Jing , Gao Jing , Ying Chunmei 
Published: May 2023

Abstract

Objective
The aim of this study was to investigate the epidemiology of bacterial vaginosis (BV) in Shanghai, China, and to explore the value of a dual-fluorescence staining method in the diagnosis of BV.

Methods
Specimens were collected from women with vaginitis at the Obstetrics and Gynecology Hospital of Fudan University from January 2020 to December 2021, and the proportions of various vaginitis types (such as Candida vaginitis, Trichomonas, and bacterial vaginitis) were analyzed statistically. To explore the diagnostic value of dual-fluorescence staining for BV, we first executed a dual-fluorescence staining method to analyze the vaginal secretions of 265 patients, then confirmed our diagnoses by consulting clinical physicians and by using Nugent scoring of Gram staining.

Results
There were 16,905 patients who were diagnosed with vaginitis over the previous 2 years, with a median age of 32 (minimum age of 9 years and maximum of 84 years). Of these patients, we noted 10,887 cases (64.40%) of BV. Our staining results revealed that the dual-fluorescence method was consistent with Gram staining in the diagnosis of BV, with a P value of less than .001 using a χ 2 test and a consistency kappa value of 0.896. Compared with Gram staining, the dual-fluorescence staining method required an acceptable time (2.2 min vs 2.5 min, respectively) and exhibited different visual effects (green and yellow vs purple and red, respectively).

Conclusion
Dual-fluorescence staining for the detection of bacterial diseases of the vagina exhibited acceptable consistency with Gram staining and performed well with respect to dyeing time, stability, and the interpretation of results. We argue that this method should be used in outpatient services.
Phylogenetic Comparative Analysis of Single-Cell Transcriptomes Reveals Constrained Accumulation of Gene Expression Heterogeneity during Clonal Expansion
Authors: Chen Feng , Li Zizhang , Zhang Xiaoyu , Wu Peng , Yang Wenjing , Yang Junnan , Chen Xiaoshu , Yang Jian-Rong 
Published: May 2023

Abstract
In the same way that a phylogeny summarizes the evolutionary history of species, a cell lineage tree describes the process of clonal expansion, in which gene expression differences between cells naturally accrue as a result of stochastic partitioning and imperfect expression control. How is functional homeostasis, a key factor in the biological function of any population of cells, maintained in the face of such continuous accumulation of transcriptomic heterogeneity remains largely unresolved. To answer this question, we experimentally determined the single-cell transcriptomes and lineage relationships of up to 50% cells in single-HEK293–seeded colonies. Phylogenetic comparative analyses of the single-cell transcriptomes on the cell lineage tree revealed three lines of evidence for the constrained accumulation of transcriptome heterogeneity among cells, including rapid saturation of transcriptomic heterogeneity upon four cell divisions, reduced expression differences within subtrees closer to expression boundaries, and cofluctuations among genes. Our analyses showcased the applicability of phylogenetic comparative methods in cell lineage trees, demonstrated the constrained accumulation of transcriptomic heterogeneity, and provided novel insight into the functional homeostasis of cell populations.
Maternal and infant microbiome: next-generation indicators and targets for intergenerational health and nutrition care
Authors: Gao Shengtao , Wang Jinfeng 
Published: May 2023

Abstract
Microbes are commonly sensitive to shifts in the physiological and pathological state of their hosts, including mothers and babies. From this perspective, the microbiome may be a good indicator for diseases during pregnancy and has the potential to be used for perinatal health monitoring. This is embodied in the application of microbiome from multi body sites for auxiliary diagnosis, early prediction, prolonged monitoring, and retrospective diagnosis of pregnancy and infant complications, as well as nutrition management and health products developments of mothers and babies. Here we summarized the progress in these areas and explained that the microbiome of different body sites is sensitive to different diseases and their microbial biomarkers may overlap between each other, thus we need to make a diagnosis prudently for those diseases. Based on the microbiome variances and additional anthropometric and physical data, individualized responses of mothers and neonates to meals and probiotics/prebiotics were predictable, which is of importance for precise nutrition and probiotics/prebiotics managements and developments. Although a great deal of encouraging performance was manifested in previous studies, the efficacy could be further improved by combining multi-aspect data such as multi-omics and time series analysis in the future. This review reconceptualizes maternal and infant health from a microbiome perspective, and the knowledge in it may inspire the development of new options for the prevention and treatment of adverse pregnancy outcomes and bring a leap forward in perinatal health care.
Narrative Asset Pricing: Interpretable Systematic Risk Factors from News Text
Authors: Bybee Leland , Kelly Bryan , Su Yinan 
Published: May 2023

AbstractWe estimate a narrative factor pricing model from news text of The Wall Street Journal. Our empirical method integrates topic modeling (LDA), latent factor analysis (IPCA), and variable selection (group lasso). Narrative factors achieve higher out-of-sample Sharpe ratios and smaller pricing errors than standard characteristic-based factor models and predict future investment opportunities in a manner consistent with the ICAPM. We derive an interpretation of the estimated risk factors from narratives in the underlying article text.Authors have furnished an Internet Appendix, which is available on the Oxford University Press Web site next to the link to the final published paper online
An immunomodulatory antibody–drug conjugate targeting BDCA2 strongly suppresses plasmacytoid dendritic cell function and glucocorticoid responsive genes
Authors: Li Xi , Zhang Yu , Li Bing , Li Jian , Qiu Yang , Zhu Zhongyuan , Hua Haiqing 
Published: May 2023

Abstract

Objectives
Blood dendritic cell antigen 2 (BDCA2) is exclusively expressed on plasmacytoid dendritic cells (pDCs) whose uncontrolled production of type I IFN (IFN-I) is crucial in pathogenesis of SLE and other autoimmune diseases. Although anti-BDCA2 antibody therapy reduced disease activity in SLE patients, its clinical efficacy needs further improvement. We developed a novel glucocorticoid receptor agonist and used it as a payload to conjugate with an anti-BDCA2 antibody to form an BDCA2 antibody–drug conjugate (BDCA2-ADC). The activation of BDCA2-ADC was evaluated in vitro.

Methods
Inhibitory activity of BDCA2-ADC was evaluated in peripheral blood mononuclear cells or in purified pDCs under ex vivo toll-like receptor agonistic stimulation. The global gene regulation in purified pDCs was analysed by RNA-seq. The antigen-dependent payload delivery was measured by reporter assay.

Results
The BDCA2-ADC molecule causes total suppression of IFNα production and broader inhibition of inflammatory cytokine production compared with the parental antibody in human pDCs. Global gene expression analysis confirmed that the payload and antibody acted synergistically to regulate both type I IFN signature genes and glucocorticoid responsive genes in pDCs.

Conclusion
Taken together, these data suggest dual mechanisms of BDCA2-ADC on pDCs and the potential for BDCA2-ADC to be the first ADC treatment for SLE in the world and a better treatment option than anti-BDCA2 antibody for SLE patients.
Serum level of galectin-9 as a potential biomarker for high risk of malignancy in dermatomyositis
Authors: Shih Yanting , Chen Shile , Huang Jie , Chen Yongheng , Zhu Zicong , Zhao Qian , Zhao Xiaoqing , Xue Feng , Xiang Jie , Chen Xiaosong , Zhu Xuemei , Pan Meng , Wu Jun , Zheng Jie , Li Hao , Cao Hua 
Published: May 2023

Abstract

Objectives
Galectin-9, as immune checkpoint protein, plays a role in regulating autoimmunity and tumour immunity. Therefore, we explored the pathophysiological link between galectin-9 and malignancy in cancer-related DM (CRDM).

Methods
Serum galectin-9 were quantified via enzyme-linked immunosorbent assay, and its association with serological indices was evaluated using Spearman analysis. Receiver operating characteristic (ROC) analysis was utilized to determine the cut-off value of galectin-9.

Results
Serum levels of galectin-9 were significantly higher in DM patients [23.38 (13.85–32.57) ng/ml] than those in healthy controls (HCs) [6.81 (5.42–7.89) ng/ml, P &lt; 0.0001], and were positively correlated with the cutaneous dermatomyositis disease area severity index activity (CDASI-A) scores (rs=0.3065, P = 0.0172). DM patients with new-onset and untreated cancer (new-CRDM) [31.58 (23.85–38.84) ng/ml] had higher levels of galectin-9 than those with stable and treated cancer (stable-CRDM) [17.49 (10.23–27.91) ng/ml, P = 0.0288], non-cancer-related DM (non-CRDM) [21.05 (11.97–28.02) ng/ml, P = 0.0258], and tumour patients without DM [7.46 (4.90–8.51) ng/ml, P &lt; 0.0001]. Serum galectin-9 levels significantly decreased [27.79 (17.04–41.43) ng/ml vs 13.88 (5.15–20.37) ng/ml, P = 0.002] after anti-cancer treatment in CRDM patients. The combination of serum galectin-9 and anti-transcriptional intermediary factor 1-γ (anti-TIF1-γ) antibody (AUC = 0.889, 95% CI 0.803–0.977) showed the highest predictive value for the presence of cancer in DM.

Conclusion
Increased galectin-9 levels were related to tumor progression in CRDM, and galectin-9 was downregulated upon cancer treatment. Monitoring serum galectin-9 levels and anti-TIF1-γ antibodies might be an attractive strategy to achieve tumour diagnosis and predict CRDM outcome.
Ewes with higher embryo survival rear lambs that grow faster
Authors: Shorten Paul R , O’Connell Anne R , Juengel Jenny L 
Published: May 2023

AbstractA key economic driver of a meat producing sheep flock is the total kilograms of lamb liveweight at weaning per ewe exposed to the ram. Optimization of key reproductive steps is required to achieve peak performance of a sheep flock. The goal of this paper was to use more than 56,000 records from a commercial flock to investigate the key reproductive steps affecting flock reproductive performance. We also applied a maximum-likelihood based technique to predict the embryo survival and ovulation rate for daughters of individual sires based on measurements of the number of fetuses at midpregnancy (detected by ultrasound-scanning). The model was used to determine how changes in premating liveweight, age, predicted ovulation rate, embryo survival, number of fetuses at midpregnancy, lamb survival, and lamb growth rate affect the total lamb liveweight at weaning per ewe exposed to the ram in the flock. The data from the commercial flock was also used to investigate the role of ewe age and premating liveweight on each reproductive step. Sensitivity analyses were performed to identify the key reproductive steps affecting flock reproductive performance. The elasticity for embryo survival was 80% of that for lamb survival. There was also significant between sire variance in the estimates of ovulation rate and embryo survival. The reproductive performance of daughters of sires with high (top 50%) and low (bottom 50%) embryo survival was investigated. Embryo survival was 0.88 in the high group and 0.82 in the low group (a 6% reduction in embryo survival). The expected total weight of lambs weaned per ewe exposed to the ram was 42 kg in the high embryo survival group and 37 kg in the low embryo survival group (a 12% reduction in the total weight of lambs weaned per ewe exposed to the ram). The proportion of twin litters was 70% in the high group and 60% in the low group, highlighting the potential importance of embryo survival for the rate of twinning in flocks with ovulation rates greater than two ova. Although lamb survival was similar between the high and low embryo survival groups, lamb growth was reduced by 10% in the low embryo survival group for the same litter size (P &lt; 0.001). This novel positive phenotypic association between embryo survival and lamb growth rate can potentially be exploited to improve flock performance.
Multivariate associations between dopamine receptor availability and risky investment decision-making across adulthood
Authors: Green Mikella A , Crawford Jennifer L , Kuhnen Camelia M , Samanez-Larkin Gregory R , Seaman Kendra L 
Published: May 2023

AbstractEnhancing dopamine increases financial risk taking across adulthood but it is unclear whether baseline individual differences in dopamine function are related to risky financial decisions. Here, thirty-five healthy adults completed an incentive-compatible risky investment decision task and a PET scan at rest using [11C]FLB457 to assess dopamine D2-like receptor availability. Participants made choices between a safe asset (bond) and a risky asset (stock) with either an expected value less than the bond (“bad stock”) or expected value greater than the bond (“good stock”). Five measures of behavior (choice inflexibility, risk seeking, suboptimal investment) and beliefs (absolute error, optimism) were computed and D2-like binding potential was extracted from four brain regions of interest (midbrain, amygdala, anterior cingulate, insula). We used canonical correlation analysis to evaluate multivariate associations between decision-making and dopamine function controlling for age. Decomposition of the first dimension (r = 0.76) revealed that the strongest associations were between measures of choice inflexibility, incorrect choice, optimism, amygdala binding potential, and age. Follow-up univariate analyses revealed that amygdala binding potential and age were both independently associated with choice inflexibility. The findings suggest that individual differences in dopamine function may be associated with financial risk taking in healthy adults.
Bilateral transcranial direct‐current stimulation promotes migration of subventricular zone‐derived neuroblasts toward ischemic brain
Authors: Lei Ruixue , Wang Shu , Liu Anchun , Cheng Jing , Zhang Zhifeng , Ren Jinyang , Yao Xujin , Kong Xiangyi , Ma Wenlong , Che Fengyuan , Chen Juan , Wan Qi 
Published: May 2023

AbstractIschemic insult stimulates proliferation of neural stem cells (NSCs) in the subventricular zone (SVZ) after stroke. However, only a fraction of NSC‐derived neuroblasts from SVZ migrate toward poststroke brain region. We have previously reported that direct‐current stimulation guides NSC migration toward the cathode in vitro. Accordingly, we set up a new method of transcranial direct‐current stimulation (tDCS), in which the cathodal electrode is placed on the ischemic hemisphere and anodal electrode on the contralateral hemisphere of rats subjected to ischemia–reperfusion injury. We show that the application of this bilateral tDCS (BtDCS) promotes the migration of NSC‐derived neuroblasts from SVZ toward the cathode direction into poststroke striatum. Reversing the position of the electrodes blocks the effect of BtDCS on the migration of neuroblasts from SVZ. BtDCS protects against neuronal death and improves the functional recovery of stroke animals. Thus, the migration of NSC‐derived neuroblasts from SVZ toward poststroke brain region contributes to the effect of BtDCS against ischemia‐induced neuronal death, supporting a potential development of noninvasive BtDCS as an endogenous neurogenesis‐based stroke therapy.
Advances in the Management of Cardiogenic Shock
Authors: Jentzer Jacob C. , Pöss Janine , Schaubroeck Hannah , Morrow David A. , Hollenberg Steven M. , Mebazaa Alexandre 
Published: May 2023

OBJECTIVES:
To review a contemporary approach to the management of patients with cardiogenic shock (CS).

DATA SOURCES:
We reviewed salient medical literature regarding CS.

STUDY SELECTION:
We included professional society scientific statements and clinical studies examining outcomes in patients with CS, with a focus on randomized clinical trials.

DATA EXTRACTION:
We extracted salient study results and scientific statement recommendations regarding the management of CS.

DATA SYNTHESIS:
Professional society recommendations were integrated with evaluated studies.

CONCLUSIONS:
CS results in short-term mortality exceeding 30% despite standard therapy. While acute myocardial infarction (AMI) has been the focus of most CS research, heart failure-related CS now predominates at many centers. CS can present with a wide spectrum of shock severity, including patients who are normotensive despite ongoing hypoperfusion. The Society for Cardiovascular Angiography and Intervention Shock Classification categorizes patients with or at risk of CS according to shock severity, which predicts mortality. The CS population includes a heterogeneous mix of phenotypes defined by ventricular function, hemodynamic profile, biomarkers, and other clinical variables. Integrating the shock severity and CS phenotype with nonmodifiable risk factors for mortality can guide clinical decision-making and prognostication. Identifying and treating the cause of CS is crucial for success, including early culprit vessel revascularization for AMI. Vasopressors and inotropes titrated to restore arterial pressure and perfusion are the cornerstone of initial medical therapy for CS. Temporary mechanical circulatory support (MCS) is indicated for appropriately selected patients as a bridge to recovery, decision, durable MCS, or heart transplant. Randomized controlled trials have not demonstrated better survival with the routine use of temporary MCS in patients with CS. Accordingly, a multidisciplinary team-based approach should be used to tailor the type of hemodynamic support to each individual CS patient’s needs based on shock severity, phenotype, and exit strategy.