bluuebunny/crossref_metadata_2025_split huggingface dataset:
The Home as a Place for Work
Authors: Ågren Maria , Wærn-Carlsson Carl Mikael , Jansson Karin Hassan , Kardell Örjan 
Published: January 2025

Abstract
This chapter discusses the home as a place of work in the period 1720 to 1880. It starts by asking what people at the time meant when they talked about ‘home’ and shows that the word was used to designate a place that offered shelter and protection rather than privacy. With time, people tended to talk more and more about their homes. The chapter analyses practices taking place in homes and demonstrates that both women and men performed much home-based work, but women were more often observed working in their own homes while men were observed in the homes of others. Nearly all forms of work could take place in home environments, not only ‘housework’ (as conventionally defined). The chapter demonstrates that women did more cleaning and washing than men, but maintains that because this was before the introduction of ideas of cleanliness and domestic hygiene, it is unlikely that housework took up a large share of women’s total time. Women and men could earn money at home by renting out rooms, particularly in the city. In this way, the domestic sphere was commercialized rather than private.
Conclusion
Authors: Jansson Karin Hassan , Lindström Jonas , Ågren Maria 
Published: January 2025

Abstract
This chapter shows how, to what extent, and why the division of work between men and women changed in the transition from early modern to modern society. It summarizes the main empirical findings and evaluates the usefulness of the verb-oriented method for studies of the nineteenth century. The chapter analyses the verb data from the period 1720 to 1880 with the help of a statistical method (Duncan segregation index) and compares with verb data from the period 1550 to 1799. The analysis shows that over time, the importance of marital status for people’s work patterns declined and, conversely, the importance of gender increased. The change was not dramatic but irrefutable, and it chimes with the other evidence presented in the book. By way of explanation, the chapter points to increasing social differentiation and shifts in labour relations. The growing impact of gender was not the result of a trickling down of new middle-class ideals, nor the effect of new preferences, nor a consequence of a separation of home and work. Instead, differences grew because more people were in a position where someone else—an employer—controlled how they used their time. The chapter puts this change into a larger theoretical perspective with the help of the concepts patriarchy, women’s status, and forced labour (Judith Bennett, Marcel van der Linden).
tttrlib: modular software for integrating fluorescence spectroscopy, imaging, and molecular modeling
Authors: Peulen Thomas-Otavio , Hemmen Katherina , Greife Annemarie , Webb Benjamin M , Felekyan Suren , Sali Andrej , Seidel Claus A M , Sanabria Hugo , Heinze Katrin G 
Published: January 2025

Abstract

Summary
We introduce software for reading, writing and processing fluorescence single-molecule and image spectroscopy data and developing analysis pipelines to unify various spectroscopic analysis tools. Our software can be used for processing multiple experiment types, e.g. for time-resolved single-molecule spectroscopy, laser scanning microscopy, fluorescence correlation spectroscopy and image correlation spectroscopy. The software is file format agnostic and processes multiple time-resolved data formats and outputs. Our software eliminates the need for data conversion and mitigates data archiving issues.

Availability and implementation
tttrlib is available via pip (https://pypi.org/project/tttrlib/) and bioconda while the open-source code is available via GitHub (https://github.com/fluorescence-tools/tttrlib). Presented examples and additional documentation demonstrating how to implement in vitro and live-cell image spectroscopy analysis are available at https://docs.peulen.xyz/tttrlib and https://zenodo.org/records/14002224.
Soft skills during residency in spain: an analysis of specialised training programmes
Authors: Sánchez-Peralta L F , Pagador J B , Sánchez-Margallo F M 
Published: January 2025

Abstract

Introduction
The significance of soft skills in reducing errors and improving clinical practice has become increasingly evident. Training in these skills should form an integral part of residency programmes.

Methods
The specialised training programmes for Health Sciences approved by the National Council of Specialties in Health Sciences were reviewed to evaluate the inclusion of five soft skills: communication, teamwork, leadership, situational awareness, and decision-making. A systematic keyword search was conducted across all programme documents.

Results
Most of the training programmes were published in 2006 or 2007 (23 programmes, 39%), with only 3 (5%) published in the last three years. Communication (35 programmes, 59.3%) and teamwork (23 programmes, 39.0%) were the most commonly mentioned skills, followed by decision-making (15 programmes, 25.4%) and leadership (11 programmes, 18.6%). Situational awareness was referenced in just one programme (1.7%). None of the programmes addressed all five skills, and only 4 (6.8%) covered four of them. In contrast, 17 programmes (28.8%) did not include any of the skills.

Conclusion
The inclusion of soft skills varies greatly across specialised training programmes. Given their proven importance in clinical practice, it is essential to develop targeted training tools, such as micro-credentials, to certify and enhance competency in these areas.
Intracoronary cardiosphere-derived cell secretome therapy: effects on ventricular tachycardia inducibility and cardiac function in a swine model
Authors: Báez Díaz C , Torrescusa A , Sánchez Margallo F M , Vázquez López F , Pulido M , López Esther , Arenal Maíz Á , Crisóstomo Ayala Y V 
Published: January 2025

Abstract

Introduction
Following myocardial infarction (MI), there is a high risk of sudden cardiac death due to the development of ventricular tachycardia (VT). This study aimed to evaluate the effects of intracoronary (IC) administration of cardiosphere-derived cell secretome (S-CDCs) on VT inducibility and cardiac function in a porcine model of MI.

Methods
Fourteen pigs underwent endovascular MI induction. At four weeks, either saline (CON; 5 ml; n=7) or S-CDCs (9.16 mg protein in 5 ml saline; n=7) were blindly administered via the IC route. VT inducibility and magnetic resonance (MR) studies were performed both pre- and four months post-IC therapy, assessing ejection fraction (EF), infarct size (%MI), and indexed end-diastolic and end-systolic volumes (VDFi, VSFi).

Results
IC therapy was successfully performed in all cases. While VT was inducible in 100% of animals prior to IC therapy, the inducibility rate was lower in the S-CDCs group compared to the CON group at four months (57% versus 100%, p=0.05). Additionally, in the S-CDCs group, EF was higher (35±10% versus 29±10%, p=NS), and ventricular volumes were lower (83±18 ml/m² and 56±20 ml/m² versus 88±29 ml/m² and 64±20 ml/m², p=NS). Furthermore, %MI was significantly reduced in the S-CDCs group (12±3% versus 16±3%, p=0.03).

Conclusion
IC therapy with S-CDCs appears to reduce post-MI VT development and suggests beneficial effects on cardiac function, including a reduction in %MI, in this swine model.
Rat model of nerve regeneration
Authors: Vela F J , Campos J L , Pires L C , Sánchez-Margallo F M , Abellán E 
Published: January 2025

Abstract

Introduction
Peripheral nerve injuries affect numerous patients annually, with outcomes influenced by factors such as the nerve’s regenerative capacity, injury severity, and the surgeon’s technical skill. Animal models are essential for refining surgical techniques and exploring new therapies to improve nerve regeneration. The choice of animal model depends on the target nerve. This study describes a standardized rat sciatic nerve defect model.

Methods
The sciatic nerve defect model involves the rat under deep anesthesia. The lateral thigh area is shaved and disinfected with chlorhexidine. A 3 cm incision is made below the femur, followed by the identification of the superficial gluteal and biceps femoris muscles using scissors and forceps. The sciatic nerve, located between the two muscle heads, is isolated by cutting the fascia and dissecting the muscle heads. A background band is placed to isolate the nerve from surrounding structures. After implementing the selected technique and treatment, the incision is closed in layers. For skin closure, intradermal sutures are used to prevent the rats from biting protruding threads.

Results
This rat sciatic nerve defect model supports various follow-up evaluations, including the sciatic functional index, ultrasonography, electromyography, MRI, genetic studies, histopathology, immunohistochemistry, and transmission electron microscopy.

Conclusion
This rat sciatic nerve model is easy to replicate and provides a robust platform for studying nerve regeneration.
Advancing Robotic Supermicrosurgery Training: Evaluating Epigastric Vessel Utility in an Animal Model
Authors: Pires Louçã Laura C , Abellán Rubio E , Vela González F J , Campos Sánchez J L , Sánchez Margallo J A , Sánchez Margallo F M 
Published: January 2025

Abstract

Introduction
Robotic supermicrosurgery has the potential to improve complex procedures, but requires advanced training in surgical techniques and in the use of robots. This study aims to establish a suitable animal model for such training.

Methods
Two microsurgeons with 2 hours of experience on a robotic microsurgery simulator performed 24 anastomoses in superficial caudal epigastric vessels in a rat model. 12 anastomoses were performed with robotic assistance and 12 by conventional techniques. Vessel size, ischemia time and patency were evaluated immediately after anastomosis (T0) and 30 minutes later (T30).

Results
The study compared arterial and venous anastomoses in rats using robotic and conventional techniques. The average diameter of the arteries was ± 0.4 mm, and veins were ± 0.6 mm. Ischemia time was longer for robotic anastomoses (97.6 minutes for arteries and 118.5 minutes for veins) compared to conventional anastomoses (90.3 and 83.3 minutes). Robotic anastomoses showed better patency, with 6 of 6 arteries and 5 of 6 veins achieving patency at T0, and 5 of 6 arteries and 6 of 6 veins at T30. Conventional anastomoses showed lower patency rates.

Conclusion
This model is technically feasible and reliable for training in robotic supermicrosurgery. It provides the necessary skills acquisition and familiarization with the technology, improving the safety and efficacy of these specialized procedures.
Effect of early initiation of empagliflozin with or without sacubitril/valsartan in a swine model of myocardial infarction
Authors: Martínez-Falguera D , Aranyo J , Teis A , Ferrer-Curriu G , Rodriguez-Leor O , Amigó N , Bisbal F , Gálvez-Montón C 
Published: January 2025

Abstract

Introduction
Empagliflozin and sacubitril/valsartan reduce cardiovascular mortality and heart failure hospitalization rates, but the effect of early initiation of these drugs following myocardial infarction (MI) remains unknown.

Methods
Twenty-four pigs with MI were randomized to receive treatment with beta blockers (BB) (Control, n=8), empagliflozin + BB (Empa, n=8), or empagliflozin + sacubitril/valsartan + BB (Empa + Sac/Val, n=8). Cardiac function was assessed via MRI, systemic immune response via flow cytometry, and metabolic profile via nuclear magnetic resonance spectroscopy over time. At follow-up, arrhythmogenic properties, myocardial fibrosis, and vascularization were evaluated.

Results
Empagliflozin treatment reduced acute systemic inflammation, decreasing the number of circulating leukocytes 2 days post-MI (P=0.010) and CCR2+ monocytes 15 days post-MI (P=0.049), increased hepatic lipid content with a cardioprotective profile, and raised type III collagen content in the myocardial scar (P=0.023), however did not modify the arrhythmogenic properties and cardiac function parameters. Empa + Sac/Val treatment increased the number of circulating monocytes at 30 days (P=0.003), predominantly CCR2-, boosted triglyceride metabolism, lowered collagen I content in the scar (P=0.082), decreased ventricular tachycardia inducibility (P=0.025), and improved cardiac function (iLVEDV P=0.029; LVSV P=0.044).

Conclusion
Empagliflozin modulates systemic inflammation, myocardial scar collagen composition, and increases cardioprotective lipid content in the liver. The combination treatment of empagliflozin and sacubitril/valsartan modulates triglyceride metabolism, reduces collagen I in the myocardial scar, decreases arrhythmias, and improves cardiac function.
Validation of a new vascular graft: vascular permeability and endothelialization
Authors: Durán-Rey D , Sánchez-Rumbo C , Brito-Pereira R , Crisóstomo V , Lanceros-Méndez S , Sánchez-Margallo F M 
Published: January 2025

Abstract

Introduction
Cardiovascular diseases are the leading cause of death globally. Tissue-engineered vascular grafts (TEVGs) are a promising therapeutic option because they can replace or repair the biological functions of blood vessels. Poly(vinylidene fluoride) (PVDF) is a synthetic material with favorable physical and biological properties that make it suitable for developing TEVGs.

Methods
A total of 12 sheep were divided into two groups: the test group, which received PVDF electrospun TEVGs (n=6), and the control group, which received commercial expanded polytetrafluoroethylene (ePTFE) grafts (n=6). Both grafts were implanted into the right common carotid artery. After 4 weeks of follow-up, vascular permeability and endothelialization were assessed through histological analysis.

Results
Although no statistically significant differences were observed, histological analysis showed that PVDF TEVGs exhibited greater vascular permeability and higher endothelialization compared to ePTFE grafts.

Conclusion
Histological results suggest that the piezoelectric properties of PVDF foster endothelial cell growth, reduce thrombus formation, and enhance vascular permeability. Although PVDF TEVGs demonstrate potential as medical devices, further preclinical studies are necessary to evaluate their suitability for treating cardiovascular diseases.
Predictive algorithm for total small intestine length for application in bariatric surgery: data recording protocol
Authors: Fernando Trebolle J , Solano Murillo J , Lobo Poyo J , Pellicer Lostao C , Valero Sabater M , Pérez Zapata A I , Tirado Anglés G , Blas Laína J L 
Published: January 2025

Abstract

Introduction
The development of a predictive algorithm for determining total small intestine length using artificial intelligence represents a significant advancement in tailoring bariatric surgical techniques. A robust data registry is fundamental to validate the algorithm and ensure its effective application. This study aims to present a protocol (document, management, and centralization) for training the predictive algorithm.

Methods
Algorithm Development: The dataset (n=1090) was recorded in an Excel database using Filemaker for initial registration. Statistical analysis was conducted in Python (Google Colaboratory Notebook format). The methodology involved data transformation and scaling (using MinMaxScaler), clustering (unsupervised machine learning), data interpolation (machine learning with SMOTE oversampling), and algorithm modeling (XGBoost model via PyCaret).

Data Recording Protocol
An anonymous electronic document was created to centralize data collection. This document includes sociodemographic data, medical history, type of bariatric surgery, and measurements of intestinal loop lengths. New data and variables are progressively added to the database for validation and testing using the K-means clustering model.

Results
The registration document will present sociodemographic data, medical history, type of bariatric surgery, and the length of intestinal loops.

Conclusion
The development of a predictive algorithm for determining total small intestine length through machine learning requires a robust and comprehensive data collection process. A structured and simplified protocol ensures the efficient collection of multicentric data, supporting model training and validation. This framework facilitates the implementation of AI-driven individualization in bariatric surgery.
Validation of a new vascular graft: inflammation and hyperplasia
Authors: Durán-Rey D , Sánchez-Rumbo C , Brito-Pereira R , Crisóstomo V , Lanceros-Méndez S , Sánchez-Margallo F M 
Published: January 2025

Abstract

Introduction
Narrowing or stenosis of blood vessels induces reduced blood flow and tissue damage due to insufficient nutrient supply. Currently, tissue-engineered vascular grafts (TEVGs) are capable of replacing or repairing the biological functions of blood vessels. Considering that the electroactive effect has shown great promise for the development of grafts with biomedical applicability, poly(vinylidene fluoride) (PVDF) is a synthetic and biocompatible material that induces a specific cellular response capable of favouring tissue regeneration, thanks to its piezoelectric properties.

Methods
PVDF electrospun TEVGs were designed and implanted into the right common carotid artery (RCCA) of 6 sheep (test group). As a control group, commercial expanded polytetrafluoroethylene (ePTFE) grafts were implanted into the RCCA of 6 sheep. After 4 weeks of follow-up, a histological study was performed to analyse the inflammation and hyperplasia of the vascular grafts.

Results
Statistically significant differences were observed in inflammation, which was higher in PVDF TEVGs than in ePTFE grafts. Although no statistically significant differences were observed in hyperplasia, histology showed that PVDF TEVGs exhibited less smooth muscle cell proliferation than ePTFE grafts.

Conclusion
Inflammation is a necessary process for tissue regeneration and was more pronounced in PVDF TEVGs. In contrast, hyperplasia causes a mismatch in elasticity, influencing the lack of vascular permeability. This issue was observed more severely in commercial ePTFE grafts. Thanks to the piezoelectric properties of PVDF, this material is able to mimic the dynamic electroactive microenvironment of cells and tissues, inducing specific cellular responses. However, further preclinical studies are required to understand the specific behaviour and interaction between the piezoelectricity of PVDF and cells.
Effect of surgical activation on ovarian functionality and follicular reactivation in ovine animal model. pilot study
Authors: Sánchez-Mateos S , Fabregues F , Párraga-Ros E , Herranz-DIez C , Otero J , Alvarez I S , Domínguez J A , Sánchez Margallo F M 
Published: January 2025

Abstract

Introduction
Poor ovarian reserve (POI) is one of the main factors that affect women with fertility problems. In this study, we tested whether making small incisions in the ovarian cortex leads to changes in the biomechanics of the ovary that could result in reactivation of ovarian functionality and folliculogenesis.

Methods
Given its similarity to the female reproductive system, both anatomically and physiologically, we used 5-year-old sheep (n=3) as an animal model. Incisions were made in one of the ovaries (OD) by laparoscopy, with the other ovary left as the control (OC). To study the effect in the short, medium, and long term, oophorectomy of both ovaries was performed at 2, 4, and 6 months, respectively, with one sheep per group. Half of each ovary was fixed in 4% paraformaldehyde for histological study, a small fragment was fixed in OCT for the biomechanics study, and the remainder was used to measure the expression of the HIPO pathway genes involved in follicular reactivation.

Results
The data from the histological analysis show that the number of growing follicles was greater at 2 months, with a higher proportion of growing follicles in the operated ovary than in the control ovary (58.9% vs. 29.7%, respectively). The biomechanical study of the cortex using AFM showed a similar trend, with greater stiffness at 2 months. Gene expression is currently under analysis.

Conclusion
Surgical manipulation of the ovary resulted in short-term reactivation of folliculogenesis.
Cannabidiol as prophylactic treatment for intestinal ischaemia reperfusion injury in elderly rats
Authors: Marea-Peñalver S , Rancan L , Linillos-Pradillo B , Ortiz M , Iñigo L , Paredes S D , García-Alonso I , Herrero de la Parte B 
Published: January 2025

Abstract

Introduction
The deprivation of blood supply to an intestinal segment, followed by its subsequent restoration, triggers the condition known as intestinal ischaemia-reperfusion syndrome (i-IRI). Throughout this process, proinflammatory and oxidative mediators are produced, released, and distributed via the bloodstream, ultimately causing damage both locally and systemically. Cannabidiol (CBD) is a molecule with antioxidant and anti-inflammatory properties that regulate oxidative stress and inflammatory states. The aim of this study is to evaluate the therapeutic potential of CBD in a model of i-IRI in aged rats.

Methods
Thirty-six 12-month-old male WAG/RijHsd rats were used, divided into three groups: healthy control group (control), untreated i-IRI group (i-IRI), and CBD-treated i-IRI group (CBD). CBD (10 mg/kg) was administered via the femoral route 30 minutes before reperfusion. Blood samples and intestinal biopsies were taken from six animals in each group, while the other six animals underwent the D-xylose absorption test to evaluate intestinal absorptive function.

Results
Treatment with CBD significantly reduced the degree of histological injury (DHI) compared to the untreated group (1.78 ± 1.23 vs. 4.5 ± 0.56; p&lt;0.001). It also improved intestinal absorptive function (control group: 2.08 ± 0.16; untreated: 0.96 ± 0.06; CBD: 1.74 ± 0.06 µg/mL; p&lt;0.001).

Conclusion
The administration of CBD prior to the restoration of the ischaemic insult was effective in reducing damage to the intestinal mucosa and improving absorptive capacity.
Prediction by bioinformatics analysis of the ability of micrornas in the secretome of menstrual blood-derived stem cells (menscs) as a treatment for ENDOMETRiosis
Authors: Suárez Carrasco I , De Pedro Muñoz M Á , Pulido Fresneda M , Álvarez Pérez V , Marchena López A M , Sánchez Margallo F M , López Nieto E 
Published: January 2025

Abstract

Introduction
Hormonal therapies and laparoscopic surgery are currently the gold standard treatments for endometriosis. However, their effectiveness is limited, with high recurrence rates observed in treated patients. This underscores the need for novel therapeutic approaches. This study aims to evaluate, in silico, the potential therapeutic effects of microRNAs present in the secretome released by menstrual blood-derived stem cells (S-MenSCs) in databases of patients with endometriosis.

Methods
MenSCs (n=3) were cultured in DMEM supplemented with 1% ITS for 96 hours. The conditioned medium was ultrafiltered (3 kDa) to isolate the secretome, and the expression of microRNAs was analysed by next-generation sequencing (NGS). Bioinformatics analysis was performed using the MalaCards database to identify differentially expressed genes (DEGs) in endometriotic tissues. The DIANA tools programme was used to assess the interaction capacity of the most abundant microRNAs with these DEGs. Cytoscape 3.8.1 was used to generate the interaction network.

Results
The analysis revealed an interaction network between 26 microRNAs identified in S-MenSCs and 24 DEGs identified in endometriosis. Each DEG interacted with at least three or more microRNAs, creating a network of numerous interactions. This suggests that the secretome may have the potential to modulate gene expression associated with endometriosis pathology.

Conclusion
MicroRNAs present in S-MenSCs may offer the potential to modulate the altered gene response in endometriosis. However, further studies are required to validate these findings and explore their clinical applications.