bluuebunny/crossref_metadata_2025_split huggingface dataset:
Comprehensive review on thermal aspects of nonthermal irreversible electroporation
Authors: Wijayanta Agung Tri , Kurata Kosaku 
Published: May 2023

AbstractIrreversible electroporation (IRE) is an innovative cell ablation method based on the concept that the application of excessive electric pulses induces a lethal increase in the permeability of the cell membrane owing to nanoscale defects, resulting in a gentle form of necrotic cell death. Although the mechanism of cell death by IRE is primarily nonthermal, thermal effects are inevitable because electric pulses inherently generate Joule heat. The larger the applied voltage to treat a large target, the greater the Joule heating and the consequent temperature rise. Therefore, the temperature increase due to Joule heating during pulse application should be carefully controlled to minimize thermal damage. Research on IRE is an interdisciplinary endeavor incorporating health science for humanitarian relief and engineering. Therefore, this study provides a comprehensive review of the thermal aspects of IRE based on existing in vitro and in vivo experimental and numerical studies. The paper begins with an overview of IRE treatment covering the geometry and arrangement of electrodes, pulse parameters, and cell death mechanisms, followed by sections on thermal damage evaluation that summarize the significant work of experiments, analysis, and comparisons. Finally, thermal mitigation strategies, including electrode modification, lowering the IRE threshold, and modified pulsing protocols, are discussed.
China special issue on gastrointestinal tumors‐Cetuximab retreatment plus camrelizumab and liposomal irinotecan in patients with RAS wild‐type metastatic colorectal cancer: Cohort B of the phase II CRACK study
Authors: Quan Ming , Chen Jingde , Chen Zhiqin , Hai Yannan , Zhou Ying , Chao Qian , Chen Chen , Li Huajun , Wang Mei , Gao Yong 
Published: May 2023

AbstractPatients with metastatic colorectal cancer (mCRC) have poor long‐term survival. Rechallenge with anti‐epidermal growth factor receptor (anti‐EGFR) based therapy has shown certain activity as late‐line therapy. To further improve clinical outcomes, we evaluated the antitumor efficacy and safety of cetuximab in combination with camrelizumab and liposomal irinotecan in patients with RASwt mCRC pretreated with anti‐EGFR‐based therapy. Patients with RASwt mCRC who had received at least two prior systemic therapies, including anti‐EGFR‐based treatment in the metastatic or unresectable disease setting, were enrolled in cohort B. Patients were treated with cetuximab (500 mg/m2) and camrelizumab (200 mg) plus liposomal irinotecan (HR070803, 60 mg/m2) intravenously once every 2 weeks. The primary endpoint was the objective response rate (ORR) by RECIST v1.1. The secondary endpoints included disease control rate (DCR), progression‐free survival (PFS), overall survival (OS) and safety. At the data cutoff (23 November 2022), 19 patients were enrolled in the two stages, and 16 were evaluable for efficacy analyses. The ORR was 25% (95% confidence interval [CI]: 10.2%‐49.5%), and DCR was 75% (95% CI: 50.5%‐89.8%). The median PFS and OS were 6.9 (95% CI: 2.6‐11.2) and 15.1 (95% CI: 6.1‐24.0) months, respectively. Grade 3 treatment‐related adverse events (TRAEs) occurred in 15.8% (3/19) of patients. No grade ≥4 TRAEs were found in the safety population. Our study suggests that anti‐EGFR retreatment therapy with cetuximab plus camrelizumab and liposomal irinotecan (HR070803) is a promising late‐line treatment option with good antitumor activity and well‐tolerated toxicity in RASwt mCRC patients.
Considerations for post‐licensure group B streptococcus vaccine effectiveness studies
Authors: Skirrow Helen , Kajungu Dan , Le Doare Kirsty , Chantler Tracey , Kampmann Beate 
Published: May 2023

SynopsisPost‐licensure studies of a Group B streptococcal vaccines for pregnant women in low and middle‐income countries will require investment in electronic health records.
Bottlenecks and recent advancements in detecting Mycobacterium tuberculosis in patients with HIV
Authors: Lin Zixun , Sun Liqin , Wang Cheng , Wang Fuxiang , Wang Jun , Li Qian , Lu Hongzhou 
Published: May 2023

AbstractTuberculosis (TB) remains a leading cause of deaths among patients with acquired immunodeficiency syndrome patients. Early diagnosis of TB is essential for administering timely anti‐TB therapy and improving health outcomes, particularly in the people living with HIV. However, conventional techniques used to detect Mycobacterium tuberculosis have significant drawbacks: for example, sputum smear microscopy has low sensitivity, and liquid culture is time‐consuming in patients with HIV‐TB co‐infection due to low sputum production. In addition, while immunological‐based methods involving tuberculin skin testing and interferon gamma release assays are commonly used for auxiliary TB diagnosis, they are often inaccurate in immunodeficient patients. Molecular techniques such as line probe assays, Xpert MTB, and lipoarabinomannan assay are recommended for early diagnosis by World Health Organization. However, no single technique is sufficicent for diagnosing HIV/TB co‐infection, suggesting that multiple diagnostic tests should be used to detect TB. Here, we summarize the drawbacks and advantages of existing TB‐diagnostic methods, as well as their applications to diagnosing HIV/TB co‐infection. We describe newly emerging technologies such as whole genome sequencing and mass spectrometry, with the aim of providing updated guidelines and alternative strategies for TB diagnosis, and particularly HIV/TB diagnosis.
An evaluation of preference stability within MSWO preference assessments for children with autism
Authors: Melanson Isaac J. , Thomas Alexandria L. , Brodhead Matthew T. , Sipila‐Thomas Emma S. , Miranda David Ray G. , Plavnick Joshua B. , Joy Tiffany A. , Fisher Marisa H. , White‐Cascarilla Allison N. 
Published: May 2023

AbstractPrevious research has commonly evaluated preference stability over time and across multiple preference assessment administrations. No studies have evaluated shifts in preference across consecutive rounds of a single preference assessment, where rounds refer to each time the experimenter resets the stimulus‐presentation array. The purpose of the present study was to examine the stability of stimulus selections across successive rounds of a multiple‐stimulus‐without‐replacement (MSWO) preference assessment with different classes of stimuli for children with autism. The study involved a secondary data analysis and calculation of preference stability across consecutive rounds using Spearman rank‐order correlation coefficients (Spearman's ) for 17 participants across 40 MSWO preference assessments. Patterns of preference stability were observed in 24 out of the 40 assessments (60%) indicating that children's preferences in this study were slightly more likely to be classified as stable than other observed patterns of responding.
Adenylate kinase immobilized on graphene oxide impairs progression of human lung carcinoma epithelial cells through adenosinergic pathway
Authors: Hetmann Anna , Szymczak Bartosz , Czarnecka Joanna , Rusak Tomasz , Wiśniewski Marek , Wujak Magdalena , Roszek Katarzyna 
Published: May 2023

AbstractPurinergic signaling, the oldest evolutionary transmitter system, has been increasingly studied as a pivotal target for novel anti‐cancer therapies. In the present work, the developed nanobiocatalytic system consisting of adenylate kinase immobilized on graphene oxide (AK‐GO) was characterized in terms of its physicochemical and biochemical properties. We put special emphasis on the AK‐GO influence on purinergic signaling components, that is, ecto‐nucleotides concentration and ecto‐enzymes expression and activity in human lung carcinoma epithelial (A549) cells. The immobilization‐dependent modification of AK kinetic parameters allowed for the removal of ATP excess while maintaining low ATP concentrations, efficient decrease in adenosine concentration, and control of the nucleotide balance in carcinoma cells. The cyto‐ and hemocompatibility of developed AK‐GO nanobiocatalytic system indicates that it can be successfully harnessed for biomedical applications. In A549 cells treated with AK‐GO nanobiocatalytic system, the significantly decreased adenosinergic signaling results in reduction of the proliferation and migration capability of cancer cells. This finding is particularly relevant in regard to AK‐GO prospective anti‐cancer applications.
A native extracellular matrix material for tissue engineering applications: Characterization of pericardial fluid
Authors: Sönmezer Dilek , Latifoğlu Fatma , Toprak Güler , Baran Münevver 
Published: May 2023

AbstractTissue engineering applications are widely used to repair and regenerate damaged tissues and organs. A scaffold, which is an important component in tissue engineering, provides a 3D environment for cells. In this study, the usability of PF components for the production of an ideal scaffold was investigated. For this aim, pericardial fluid (PF) was harvested from the bovine heart, then its structure and components were characterized. The results of Raman spectroscopy analysis, histological staining, and scanning electron microscopy (SEM) shows that the pericardial fluid contains collagen type I and IV, elastin, fibrin, and glycosaminoglycan (GAG), which are native extracellular matrix (ECM) components. The results demonstrated that (i) PF contains native ECM proteins and GAG such as collagen types I, III, and IV, elastin, and fibrin. (ii) The PF is highly similar to the native ECM structure. (iii) PF can significantly contribute to many tissue engineering studies as a native ECM material to increase the biocompatibility of biomaterials and to several in vitro/in vivo cell culture studies. (iv) PF containing multiple ECM molecules, can be used alone or together with hyaluronic acid, poly(ethylene glycol) (PEG), alginate, chitosan, matrigel, and gelatin methacryloyl (GelMA) materials in bioprinting systems for eliminating the disadvantages of these materials.
Quantitative MRI in the Local Staging of Prostate Cancer: A Systematic Review and Meta‐Analysis
Authors: Xiao Vieley G. , Kresnanto Jordan , Moses Daniel A. , Pather Nalini 
Published: May 2023

BackgroundLocal staging of prostate cancer (PCa) is important for treatment planning. Radiologist interpretation using qualitative criteria is variable with high specificity but low sensitivity. Quantitative methods may be useful in the diagnosis of extracapsular extension (ECE).PurposeTo assess the performance of quantitative MRI markers for detecting ECE.Study TypeSystematic review and meta‐analysis.Subjects4800 patients from 28 studies with histopathologically confirmed PCa on radical prostatectomy were pooled for meta‐analysis. Patients from 46 studies were included for systematic review.Field Strength/SequenceDiffusion‐weighted, T2‐weighted, and dynamic contrast‐enhanced MRI at 1.5 T or 3 T.AssessmentPubMed, Embase, Web of Science, Scopus, and Cochrane databases were searched to identify studies on diagnostic test accuracy or association of any quantitative MRI markers with ECE. Results extracted by two independent reviewers for tumor contact length (TCL) and mean apparent diffusion coefficient (ADC‐mean) were pooled for meta‐analysis, but not for other quantitative markers including radiomics due to low number of studies available.Statistical TestsHierarchical summary receiver operating characteristic (HSROC) curves were computed for both TCL and ADC‐mean, but summary operating points were computed for TCL only. Heterogeneity was investigated by meta‐regression. Results were significant if P ≤ 0.05.ResultsAt the 10 mm threshold for TCL, summary sensitivity and specificity were 0.76 [95% confidence interval (CI) 0.71–0.81] and 0.68 [95% CI 0.63–0.73], respectively. At the 15 mm threshold, summary sensitivity and specificity were 0.70 [95% CI 0.53–0.83] and 0.74 [95% CI 0.60–0.84] respectively. The area under the HSROC curves for TCL and ADC‐mean were 0.79 and 0.78, respectively. Significant sources of heterogeneity for TCL included timing of MRI relative to biopsy.Data ConclusionBoth 10 mm and 15 mm thresholds for TCL may be reasonable for clinical use. From comparison of the HSROC curves, ADC‐mean may be superior to TCL at higher sensitivities.Level of Evidence3Technical Efficacy Stage2
Advances in hybrid format‐based neuro‐transfer function techniques for parametric modeling of microwave components
Authors: Ma Li , Zhang Qi‐Jun , Liu Wei , Zhang Jianan 
Published: May 2023

AbstractElectromagnetic (EM) parametric modeling has become significant for EM designs of microwave devices. This paper outlines recent advances in hybrid format‐based neuro‐transfer function (TF) techniques for EM parametric modeling of microwave devices. To solve the problem of high‐sensitivity, a novel decomposition approach is discussed to develop a rational‐based neuro‐TF model of EM behavior of microwave devices. To handle the issue of non‐smoothness and discontinuity, a parametric modeling technique incorporating pole‐residue/rational and neural network hybrid transfer function (short for rational/pole‐residue hybrid neuro‐TF) of EM behavior is reviewed. This technique effectively combines rational and residue‐pole formats of the transfer functions. Compared with the residue‐pole‐based neuro‐TF modeling approach and the rational‐based neuro‐TF modeling approach, the rational/pole‐residue hybrid neuro‐TF technique allows for better accuracy in large geometrical changes and high order applications. A parametric modeling method combining neural network and polynomial‐transfer function (neuro‐PTF) is further presented as an advanced version of the rational/pole‐residue hybrid neuro‐TF method. In this approach, the pole–residue‐based transfer function and the polynomial function are used together to describe the EM responses, and can produce more accurate models, especially with large geometrical variables. Following the modeling process, trained models can be used to provide fast and accurate EM response predictions and can subsequently be used for advanced circuit and system design.
Design of outdoor LED ground display system in major scenes
Authors: Yu Chao , Wu Yunpeng , Yang Fan , Wu Jian , Leng Changlin , Song Yang 
Published: May 2023

AbstractLED ground display system, as an important outdoor display system in major scenes such as some international performance events or interactive display system, has brought spectacular and gorgeous artistic effect to the audience. According to the customized design and the effort we made, this world class super large‐scale outdoor display system has realized successfully at the beginning of the year 2022 in Beijing. This LED ground display system has many innovation in product design, control system design, and field implementation especially for the need of super high reliability, high environmental suitability, convenient installability, 4,500 m2 super large display area, very complex system integration, and long service time. This paper indicates the detail introduction of the ground display system, specially focuses on the high reliability product design, high display performance design, system reliability design, field implementation, and actual verification results of the semi‐circular display area on both sides of the stage. Meanwhile, some simulation technologies are used in this design related to optics, mechanics, and so on. Furthermore, under the special application environment, to ensure the reliability of the outdoor display system, a dynamic screen preheating method according to temperature change is proposed and proved effectively by practice.
Sp1‐mediated miR‐193b suppresses atopic dermatitis by regulating HMGB1
Authors: Liu Ying‐Ke , Liu Lei‐Shan , Zhu Bo‐Chen , Chen Xiu‐Fang , Tian Li‐Hong 
Published: May 2023

AbstractAtopic dermatitis (AD) is a chronic and recurrent inflammatory skin disease. Keratinocyte dysfunction plays a central role in AD development. MicroRNA is a novel player in many inflammatory and immune skin diseases. In this study, we investigated the potential function and regulatory mechanism of miR‐193b in AD. Inflamed human keratinocytes (HaCaT) were established by tumor necrosis factor (TNF)‐α/interferon (IFN)‐γ stimulation. Cell viability was measured using MTT assay, while the cell cycle was analyzed using flow cytometry. The cytokine levels were examined by enzyme‐linked immunosorbent assay. The interaction between Sp1, miR‐193b, and HMGB1 was analyzed using dual luciferase reporter and/or chromatin immunoprecipitation (ChIP) assays. Our results revealed that miR‐193b upregulation enhanced the proliferation of TNF‐α/IFN‐γ‐treated keratinocytes and repressed inflammatory injury. miR‐193b negatively regulated high mobility group box 1 (HMGB1) expression by directly targeting HMGB1. Furthermore, HMGB1 knockdown promoted keratinocyte proliferation and inhibited inflammatory injury by repressing nuclear factor kappa‐B (NF‐κB) activation. During AD progression, HMGB1 overexpression abrogated increase of keratinocyte proliferation and repression of inflammatory injury caused by miR‐193b overexpression. Moreover, transcription factor Sp1 was identified as the biological partner of the miR‐193b promoter in promoting miR‐193b expression. Therefore, Sp1 upregulation promotes keratinocyte proliferation and represses inflammatory injury during AD development via promoting miR‐193b expression and repressing HMGB1/NF‐κB activation.
Factors Associated With Loss of Follow‐Up in Transgender Patients Receiving Vocal Therapy
Authors: So Raymond J. , Davis Ashley , Akst Lee M. , Hillel Alexander T. , Best Simon R. 
Published: May 2023

ObjectivesOptimal vocal care for transgender patients necessitates regular follow‐up. Factors associated with loss of follow‐up in voice patients have never been investigated. In this study, we report a case series of transgender patients seeking vocal care at our institution and compare those who were and were not lost to follow‐up.MethodsCharts of transgender patients diagnosed with gender dysphoria who sought vocal care at our institution from January 2018 through May 2022 were reviewed. A chronological timeline of each patient's care at our vocal clinic was recorded. Loss of follow‐up was defined as instances in which patients were not yet satisfied with their vocal outcomes and expressed interest in scheduling a subsequent visit but had not yet done so. Logistic regressions were performed to identify factors associated with loss of follow‐up.ResultsOf 73 patients identified, 59 (80.8%) were assigned male at birth, and 72 (98.6%) were non‐Hispanic White. Loss of follow‐up occurred in 35 (47.9%) patients. Patients who received vocal surgery were significantly less likely to be lost to follow‐up (OR: 0.16 (0.03, 0.79); p = 0.03). The availability of telemedicine options for vocal care was protective against loss of follow‐up (OR: 0.09 (0.02, 0.44); p = 0.003). Patients who received other non‐voice gender‐affirming treatments concomitant to their vocal care were more likely to be lost to follow‐up (OR: 4.44 (1.35, 14.59); p = 0.01).ConclusionLoss of follow‐up in transgender patients receiving vocal care is common. Providing telemedicine options and encouraging patients to complete vocal care prior to or after receiving other non‐voice gender‐affirming treatments may help increase rates of follow‐up.Level of Evidence4 Laryngoscope, 133:3061–3067, 2023
Diversity of soil mites (Acari: Mesostigmata) in streamside mountain forests
Authors: Kamczyc Jacek , Skorupski Maciej , Dyderski Marcin K. , Horodecki Paweł , Rawlik Mateusz , Jagodziński Andrzej M. 
Published: May 2023

AbstractHabitat loss and fragmentation are among the most influential factors responsible for recent biodiversity decline. The key to understanding the changes in ecosystem functioning is the analysis of the relationship between species diversity and environmental gradients such as those found near streamside forests. We used soil mites (Mesostigmata) as model species as they are predators in soil ecosystems, they occur in high relative abundance, their communities are species‐rich and are robust toward sampling and extraction methods. The main goals of this study were to (1) check how the plant community and associated environmental variables (light conditions, litter accumulation, and soil acidity) along mountain streams affect soil Mesostigmata abundance and diversity, and (2) to analyze the distance from mountain streams to which the ecosystem functions (Mesostigmata diversity) are affected, based on a distance gradient. We systematically established 31 study plots (500 m2) every 200 m along the watercourses, representing three habitat types along the stand composition gradient (ash‐sycamore [Fraxinus excelsior–Acer pseudoplatanus], European beech [Fagus sylvatica], and Norway spruce [Picea abies] forests) for measurements of soil litter thickness, soil acidity, and light conditions. We extracted soil Mesostigmata mites from samples in Tullgren funnels and identified them to the species level. We found that Mesostigmata abundance in forests decreased in the following order: Norway spruce forest > European beech forest > ash‐sycamore forest. Additionally, changes in Shannon diversity were associated with light conditions. In conclusion, streamside mountain forests maintained abundant, diverse and species‐rich Mesostigmata communities, which were associated with a plant community‐light condition gradient. Additionally, more abundant mite communities occurred in forests with higher soil pH values, and under higher light conditions.
Linking microbial community structure to function underneath moss‐dominated biocrusts in rare earth elements mine areas under a subtropical climate
Authors: Kong Zhaoyu , Zhong Hui , Jin Xin , Cai Qiying , Zou Zhengwei , Ge Gang , Wu Lan 
Published: May 2023

AbstractRare earth elements (REEs) exploitation has caused large‐scale desertification. Biocrusts and their microbial community are crucial for the restoration and revegetation of abandoned mine areas. However, little is known about how microbial communities with biocrusts contribute to the sustainable function of degraded mine lands. Here, we evaluated soil nutrients, microbial community structure, and enzyme activities (involved in C, N, and P cycling) underneath moss biocrusts in REEs mine lands in Jiangxi Province, China. The results showed that the dissimilarities in the diversity, and structure of bacteria/fungi community with and without moss biocrusts were significant. Nevertheless, the effects of moss biocrusts on the abundant fractions of bacterial/fungal communities depend on land use types. Moreover, network hubs affiliated with Gammaproteobacteria (OTU7263) and Geodermatophilus sp. (OTU7087) were identified from moss networks, but no hub was found in bare soil. More importantly, the microbial communities underneath moss biocrusts mainly had direct effects on enzyme activities, while the microbial communities in bare soils had a relatively weak direct influence on enzyme activities, and most effects were indirectly driven via soil abiotic properties. Our results contribute to a better understanding of microbial community and corresponding ecological functions with moss biocrusts in degraded mine lands.