bluuebunny/crossref_metadata_2025_split huggingface dataset:
Molecular basis of sulfolactate synthesis by sulfolactaldehyde dehydrogenase fromRhizobium leguminosarum
Authors: Li Jinling , Sharma Mahima , Meek Richard , Alhifthi Amani , Armstrong Zachary , Soler Niccolay Madiedo , Lee Mihwa , Goddard-Borger Ethan D. , Blaza James N. , Davies Gideon J. , Williams Spencer J. 
Published: March 2023

AbstractSulfolactate (SL) is a short-chain organosulfonate that is an important reservoir of sulfur in the biosphere. SL is produced by oxidation of sulfolactaldehyde (SLA), which in turn derives from sulfoglycolysis of the sulfosugar sulfoquinovose, or through oxidation of 2,3-dihydroxypropanesulfonate. Oxidation of SLA is catalyzed by SLA dehydrogenases belonging to the aldehyde dehydrogenase superfamily. We report that SLA dehydrogenaseRlGabD from the sulfoglycolytic bacteriumRhizobium leguminsarumSRDI565 can use both NAD+and NADP+as cofactor to oxidize SLA, and indicatively operates through a rapid equilibrium ordered mechanism. We report the cryo-EM structure ofRlGabD bound to NADH, revealing a tetrameric quaternary structure and supporting proposal of organosulfonate binding residues in the active site, and a catalytic mechanism. Sequence based homology searches identified SLA dehydrogenase homologs in a range of putative sulfoglycolytic gene clusters in bacteria predominantly from the phyla Actinobacteria, Firmicutes, and Proteobacteria. This work provides a structural and biochemical view of SLA dehydrogenases to complement our knowledge of SLA reductases, and provide detailed insights into a critical step in the organosulfur cycle.Graphical abstractSulfolactate is an important species in the biogeochemical sulfur cycle. Herein we report the 3D cryo-EM structure and kinetics of its biosynthetic enzyme, sulfolactaldehyde dehydrogenase.
Interpretable full-epoch multiclass decoding for M/EEG
Authors: Csaky Richard , van Es Mats W.J. , Jones Oiwi Parker , Woolrich Mark 
Published: March 2023

AbstractMultivariate pattern analysis (MVPA) of Magnetoencephalography (MEG) and Electroencephalography (EEG) data is a valuable tool for understanding how the brain represents and discriminates between different stimuli. Identifying the spatial and temporal signatures of stimuli is typically a crucial output of these analyses. Such analyses are mainly performed using linear, pairwise, sliding window decoding models. These allow for relative ease of interpretation, e.g. by estimating a time-course of decoding accuracy, but are computationally intensive and can have limited decoding performance. On the other hand, full epoch decoding models, commonly used for brain-computer interface (BCI) applications, can provide better decoding performance. However, they lack methods for interpreting the contributions of spatial and temporal features. In this paper, we propose an approach that combines a multiclass, full epoch decoding model with supervised dimensionality reduction, while still being able to reveal the contributions of spatiotemporal and spectral features using permutation feature importance. We demonstrate the approach on 3 different task MEG datasets using image presentations. Our results demonstrate that this approach consistently achieves higher accuracy than the peak accuracy of a sliding window decoder while estimating the relevant spatiotemporal features in the MEG signal. Finally, we show that our multiclass model can also be used for pairwise decoding, eliminating the computational burden of training separate models for each pairwise combination of stimuli.
Monomeric agonist peptide/MHCII complexes activate T-cells in an autonomous fashion
Authors: Platzer René , Hellmeier Joschka , Göhring Janett , Perez Iago Doel , Schatzlmaier Philipp , Bodner Clara , Schütz Gerhard J. , Sevcsik Eva , Stockinger Hannes , Brameshuber Mario , Huppa Johannes B. 
Published: March 2023

ABSTRACTMolecular crowding of agonist peptide/MHC class II complexes (pMHCIIs) with structurally similar, yetper senon-stimulatory endogenous pMHCIIs has been postulated to sensitize T-cells for the recognition of single antigens on the surface of dendritic cells and B-cells. When testing this premise with the use of advanced live cell microscopy, we observed pMHCIIs as monomeric, randomly distributed entities diffusing rapidly after entering the APC surface. Synaptic TCR-engagement of highly abundant endogenous pMHCIIs was low or non-existent and affected neither TCR-engagement of rare agonist pMHCII in early and advanced synapses nor agonist-induced TCR-proximal signaling. Our findings highlight the capacity of single freely diffusing agonist pMHCIIs to elicit the full T-cell response in an autonomous and peptide-specific fashion with consequences for adaptive immunity and immunotherapeutic approaches.SHORT SUMMARYPlatzer et al. revealed via highly quantitative and single molecule live cell microscopy the nature of peptide-loaded MHC class II molecules (pMHCII) as monomeric, densely populating, randomly distributed and predominantly rapidly diffusing entities on the surface of B-cells and dendritic cells. Low abundant stimulatory agonist pMHCII acted as autonomous units with the highest chance of T-cell detection when equally spread on APCs. The presence of bystander-pMHCII previously termed “co-agonist pMHC” affected neither synaptic agonist -TCR-binding nor efficiencies of T-cell recognition. “Co-agonist”-TCR-binding resembled random molecular collisions. Findings inform the design of T-cell-based immunotherapies.
Pten is a disconnecting node in the molecular landscape of the proliferation/quiescence decision during mammary gland acinogenesis
Authors: Tomasin Rebeka , Rodrigues Ana Maria , Manucci Antonio Carlos , Bruni-Cardoso Alexandre 
Published: March 2023

AbstractCell context is key for cell phenotype. Using physiologically relevant models of laminin-rich ECM (lrECM) induction of mammary epithelial cell quiescence and differentiation, we have provided a landscape of the status of key molecular players involved in the proliferation/quiescence decision. Repression of some positive regulators of the cell cycle, such as cyclins and CDKs, occurred already at the mRNA level, whereas negative regulators of the cell cycle, such as Pten and p27, were upregulated only at the protein level. Interestingly, cell cycle arrest occurred despite the active status of Fak, Src and PI3k, because their downstream proliferative signalling pathways were repressed, suggesting the existence of a disconnecting node between upstream and downstream proliferative signalling in quiescent cells. Pten fulfils this role. Inhibition of Pten increased proliferation and restored Akt/mTORC1/2 and Mapk signalling in cells exposed to lrECM. In mice, Pten levels were positively correlated to the basement membrane thickness in the developing mammary epithelia, and Pten localized to the apicolateral membrane of luminal cells both in in ducts and near the nascent lumen in terminal end bud, characteristics consistent with a role for Pten in inducing and sustaining quiescence and tissue architecture. Accordingly, in 3D acininogenesis models, Pten was required for the onset and maintenance of quiescence, cell polarity and lumen assembly. The notion that lrECM-triggered differentiation involves a signalling circuitry with many layers of regulation provides an explanation for the resilience of quiescence within a growth-suppressive microenvironment, and that perturbations in master regulators, such as Pten, could disrupt the quiescent phenotype.
RTF2 controls replication repriming and ribonucleotide excision at the replisome
Authors: Conti Brooke A. , Ruiz Penelope D. , Broton Cayla , Blobel Nicolas J. , Kottemann Molly C. , Sridhar Sunandini , Lach Francis P. , Wiley Tom , Sasi Nanda K. , Carroll Thomas , Smogorzewska Agata 
Published: March 2023

AbstractGenetic information is duplicated via the highly regulated process of DNA replication. The machinery coordinating this process, the replisome, encounters many challenges, including replication fork-stalling lesions that threaten the accurate and timely transmission of genetic information. Cells have multiple mechanisms to repair or bypass lesions that would otherwise compromise DNA replication1,2. We have previously shown that proteasome shuttle proteins, DNA Damage Inducible 1 and 2 (DDI1/2) function to regulate Replication Termination Factor 2 (RTF2) at the stalled replisome, allowing for replication fork stabilization and restart3. Here we show that RTF2 regulates replisome localization of RNase H2, a heterotrimeric enzyme responsible for removing RNA in the context of RNA-DNA heteroduplexes4–6. We show that during unperturbed DNA replication, RTF2, like RNase H2, is required to maintain normal replication fork speeds. However, persistent RTF2 and RNase H2 at stalled replication forks compromises the replication stress response, preventing efficient replication restart. Such restart is dependent on PRIM1, the primase component of DNA polymerase α-primase. Our data show a fundamental need for regulation of replication-coupled ribonucleotide incorporation during normal replication and the replication stress response that is achieved through RTF2. We also provide evidence for PRIM1 function in direct replication restart following replication stress in mammalian cells.
Integrin-based adhesions promote cell-cell junction remodelling and cytoskeletal rearrangements to drive embryonic wound healing
Authors: Ly Michelle , Schimmer Clara , Hawkins Ray , Rothenberg Katheryn , Fernandez-Gonzalez Rodrigo 
Published: March 2023

ABSTRACTEmbryos have a remarkable ability to repair wounds rapidly, with no inflammation or scarring. Embryonic wound healing is driven by the collective movement of the surrounding cells to seal the lesion. During embryonic wound closure, the cells adjacent to the wound polarize the cytoskeletal protein actin and the molecular motor non-muscle myosin II, which accumulate at the wound edge forming a supracellular cable around the wound. Adherens junction proteins including E-cadherin are internalized from the interface with the lesion and localize to former tricellular junctions at the wound margin, in a process necessary for cytoskeletal polarity. Using quantitative live microscopy, we found that the cells adjacent to wounds in theDrosophilaepidermis also polarized Talin, a core component of cell-extracellular matrix (ECM) adhesions that links integrins to the actin cytoskeleton. Integrin knock-down and inhibition of integrin binding delayed wound closure and were associated with a reduction in actin levels around the wound. Additionally, disrupting integrins caused a defect in E-cadherin reinforcement at tricellular junctions along the wound edge, suggesting crosstalk between integrin-based and cadherin-based adhesions. Together, our results show that cell-ECM adhesion contributes to embryonic wound repair and reveal an interplay between cell-cell and cell-ECM adhesion in the collective cell movements that drive rapid wound healing.
Arabidopsis cell suspension culture and RNA sequencing reveal regulatory networks underlying plant programmed cell death
Authors: Burke Rory , McCabe Aideen , Sonawane Neetu Ramesh , Rathod Meet Hasmukh , Whelan Conor , McCabe Paul F. , Kacprzyk Joanna 
Published: March 2023

AbstractProgrammed cell death (PCD) facilitates targeted elimination of redundant, damaged, or infected cells via genetically controlled pathways. In plants, PCD is often an essential component of normal development and can also mediate responses to abiotic and biotic stress stimuli. However, studying the transcriptional regulation of this fundamental process is hindered by difficulties in sampling small groups of cells undergoing PCD that are often buried within the bulk of living plant tissue. We addressed this challenge by using RNA sequencing (RNA-Seq) ofArabidopsis thalianasuspension cells, a system that allows precise monitoring of PCD activation and progression. The use of three PCD-inducing treatments (salicylic acid, heat and critical dilution), in combination with three cell death modulators (3- methyladenine, lanthanum chloride and conditioned medium), allowed isolation of candidate ‘core’ and stimuli-specific PCD genes, inference of underlying gene regulatory networks and identification of putative transcriptional regulators. This analysis underscored cell cycle disturbance and the repression of both pro-survival stress responses and mitochondrial retrograde signalling as key elements of the PCD-associated transcriptional signature in plants. Further, phenotyping of twentyArabidopsisT-DNA insertion mutants in selected candidate genes confirmed a role for several in PCD and stress tolerance regulation, and validated the potential of these generated resources to identify novel genes involved in plant PCD pathways and/or stress tolerance in plants.
The ion channel Trpc6a regulates the cardiomyocyte regenerative response to mechanical stretch
Authors: Rolland Laura , Faucherre Adèle , Abaroa Jourdano Mancilla , Drouard Aurélien , Jopling Chris 
Published: March 2023

AbstractMyocardial damage caused for example by cardiac ischemia leads to ventricular volume overload resulting in increased stretch of the remaining myocardium. In adult mammals, these changes trigger an adaptive cardiomyocyte hypertrophic response which, if the damage is extensive, will ultimately lead to pathological hypertrophy and heart failure. Conversely, in response to extensive myocardial damage, cardiomyocytes in the adult zebrafish heart and neonatal mice proliferate and completely regenerate the damaged myocardium. We therefore hypothesized that in adult zebrafish, changes in mechanical loading due to myocardial damage may act as a trigger to induce cardiac regeneration. Based, on this notion we sought to identify mechanosensors which could be involved in detecting changes in mechanical loading and triggering regeneration. Here we show using a combination of knockout animals, RNAseq andin vitroassays that the mechanosensitive ion channel Trpc6a is required by cardiomyocytes for successful cardiac regeneration in adult zebrafish. Furthermore, using a cyclic cell stretch assay, we have determined that Trpc6a induces the expression of components of the AP1 transcription complex in response to mechanical stretch. Our data highlights how changes in mechanical forces due to myocardial damage can be detected by mechanosensors which in turn can trigger cardiac regeneration.
The investment dynamics in renewable energy transition in Africa: the asymmetric role of oil prices, economic growth and ICT
Authors: Evans Olaniyi 
Published: March 2023

Purpose
This study aims to investigate the effect of oil prices, economic growth and information communication technology (ICT) on investment into renewable energy transition (RET).

Design/methodology/approach
Based on six selected African countries (i.e. Algeria, Egypt, Angola, Ethiopia, South Africa and Nigeria), the study uses a nonlinear autoregressive distributed lag model over the period from 1995 to 2020.

Findings
The results show that increasing oil prices, by substitution effect, leads to increasing RET investment, while declining oil prices lead to decreasing RET investment in the short and long run. Furthermore, the results reveal that increasing real gross domestic product leads to increased RET investment, while declining real gross domestic product (GDP) leads to decreasing RET investment both in the short and long run. Simultaneously, the study shows that increasing ICT has a significant and positive impact on RET investment, while declining ICT has a significant negative impact on RET investment in the short and long run.

Originality/value
The findings of this study have advanced the understanding of which factors significantly influence RET investment and the need to concentrate efforts on strategically addressing those factors. The findings indicate that these countries are at the progressive stage in terms of renewable energy; though increasing oil prices contribute to rising RET investment, the countries can be more proactive by improving the full potential of ICT as well as facilitating the growth of their economies.
Dual networks: how does knowledge network embeddedness affect firms' supply chain learning?
Authors: Yan Ji , Yu Zihao , Fernandes Kiran , Xiong Yu 
Published: March 2023

PurposeTo explore the mechanism that shapes firms' supply chain learning (SCL) practices, this study examines the relationship between firms' knowledge network embeddedness and their SCL practice in a supply chain network, as well as the moderating role of supply chain network cohesion in this relationship.Design/methodology/approachUsing patent application data and supply chain partner information from 869 listed firms between 2011 and 2020 in China, this study uses fixed-effect regression models to reduce endogeneity problems by controlling for individual heterogeneity effects that cannot be observed over time.FindingsFirms' knowledge network embeddedness has an inverted U-shaped effect on their SCL, and this non-linear relationship is conditional on supply chain network cohesion, which strengthens (weakens) the positive (negative) effect of knowledge network embeddedness on SCL.Practical implicationsThe findings show that managers can reconcile the downsides of knowledge network embeddedness on SCL by fostering greater supply chain network cohesion.Originality/valueDrawing from the network pluralism perspective, this study contributes to supply chain literature by extending the research context of the antecedents of SCL from a single-network setting to a dual-network setting. It extends the network pluralism perspective by showing that not only positive effects but also negative effects of network embeddedness can transfer from one network to another.
International servitization: theoretical roots, research gaps and implications
Authors: Bıçakcıoğlu-Peynirci Nilay , Morgan Robert E. 
Published: March 2023

PurposeWhile the servitization concept has gained increasing attention in the domestic marketing literature, there is more limited knowledge with respect to its implications within the international context. The purpose of this paper is to examine the servitization concept in the international context considering its boundary conditions and its effects on firm performance. Relying on the resource-based view and the boundary conditions function, the authors aim to identify a set of research gaps focusing on how strategic resource decisions (i.e. slack resources and digital marketing capabilities) help industrial firms to provide different types of service offerings (i.e. services in support of product (SSPs) and services in support of client's actions (SSCs)) that leverage their performance in international markets.Design/methodology/approachThe authors illustrate international servitization strategies and the boundaries of servitization activities that firms employ through a series of case vignettes. The authors derive a conceptual framework, serving as a guideline for future research endeavors.FindingsThe authors indicate the importance of servitization strategies in international markets and identify eight research gaps, which help to build an agenda for future research. Key differences between international servitization strategies and strategic resource decisions are addressed through illustrative case vignettes from different industries.Practical implicationsThe insights from this work inform marketing executives about how international servitization strategies are influential in the context of overseas markets by characterizing the servitization concept and elaborating upon the specific resources and capabilities that underpin its execution in foreign markets.Originality/valueThis conceptual paper provides a comprehensive understanding of international servitization strategies in overseas markets and identifies several research paths that contribute to the complex nature of servitization in the international context and help scholars spot gaps and research questions worthy of investigation.
Mapping and review of self-neglect policies and procedures from safeguarding adults boards in England
Authors: Orr David 
Published: March 2023

Purpose
Local Safeguarding Adults Board (SAB) policies, procedures, guidance and related documents on self-neglect were gathered and analysed, to map what approaches are being taken across England. This paper aims to identify areas of divergence to highlight innovations or challenges faced by SABs.

Design/methodology/approach
Self-neglect documents were identified by searching SAB websites. Data were extracted into a framework enabling synthesis and comparison between documents.

Findings
This paper reports on how English SAB documentation defines self-neglect, treats executive capacity, lays out pathways for self-neglect cases, advises on refusal of service input and multi-agency coordination and draws on theories or tools. Greater coherence in understanding self-neglect has developed since it was brought within safeguarding in 2014; however, variation remains regarding scope, referral pathways and threshold criteria.

Research limitations/implications
This review was limited to published SAB documentation at one point in time and could not consider either the wider context of safeguarding guidance and training or implementation in practice.

Practical implications
This review provides an overview of how SABs are interpreting national guidance and guiding practitioners. The trends and areas of uncertainty identified offer a resource for informed research and policy-making.

Originality/value
To the best of the author’s knowledge, this is the first systematic survey of SAB self-neglect policies, procedures and guidance since self-neglect was included under safeguarding.
External corporate governance and assurance of corporate social responsibility reports: evidence from China
Authors: Liu Peiran , Li Ziyang , Luo Peng 
Published: March 2023

Purpose
This paper aims to verify whether the legitimate pressure of external forces on heavily polluting firms’ corporate social responsibility (CSR)-related behaviors affect firms’ assurance strategy in the Chinese context. The authors argue that, under external pressure, as a source of legitimacy, the assurance over CSR reports allows the business behaviors of heavy polluters to be recognized by society.

Design/methodology/approach
This paper sampled listed heavy polluters in China from 2011 to 2018 and used the multiperiod logit model to examine the effects of external corporate governance on firms’ assurance decisions. Principal component analysis methods were used to construct a comprehensive framework of external corporate governance. The indicators were obtained from the China Stock Market and Accounting Research databases, the NERI Report and the China Urban Statistical Yearbook.

Findings
This paper confirms that external corporate governance positively affects firms’ assurance decisions, and good financial conditions, well-governed internal controls and sufficient government subsidies positively moderate this effect.

Practical implications
The findings provide feasible ways to encourage firms’ high-quality corporate environmental information disclosure, thus providing valuable guidance for policymakers and other stakeholders to effectively supervise firms’ CSR behaviors.

Social implications
The findings are of great importance in encouraging high-quality corporate environmental information disclosures, improving the support of capital markets among developing countries and drawing social attention to the environmental protection and social responsibility of heavy polluters.

Originality/value
The research extends the current research in the field of social environmental accounting by using legitimacy theory to explain firms’ assurance motivations. Additionally, this paper focuses on the practices of assurance services in the emerging economy and provides suggestions for developing assurance over CSR reports.