bluuebunny/crossref_metadata_2025_split huggingface dataset:
Efficient malachite green biodegradation by Pseudomonas plecoglossicide MG2: process optimization, application in bioreactors, and degradation pathway
Authors: El-Bendary Magda A. , Fawzy Mariam E. , Abdelraof Mohamed , El-Sedik Mervat , Allam Mousa A. 
Published: September 2023

AbstractMicrobial degradation of synthetic dyes is considered a promising green dye detoxification, cost-effective and eco-friendly approach. A detailed study on the decolorization and degradation of malachite green dye (MG) using a newly isolated Pseudomonas plecoglossicide MG2 was carried out. Optimization of MG biodegradation by the tested organism was investigated by using a UV–Vis spectrophotometer and the resultant degraded products were analyzed by liquid chromatography–mass spectrometry and FTIR. Also, the cytotoxicity of MG degraded products was studied on a human normal retina cell line. The optimum conditions for the significant maximum decolorization of MG dye (90–93%) by the tested organism were pH 6–7, inoculum size 4–6%, and incubation temperature 30–35 °C, under static and aerobic conditions. The performance of Pseudomonas plecoglossicide MG2 grown culture in the bioreactors using simulated wastewater was assessed. MG degradation (99% at 100 and 150 mg MG/l at an optimal pH) and COD removal (95.95%) by using Pseudomonas plecoglossicide MG2 culture were the best in the tested culture bioreactor in comparison with that in activated sludge or tested culture-activated sludge bioreactors.The FTIR spectrum of the biodegraded MG displayed significant spectral changes, especially in the fingerprint region 1500–500 as well as disappearance of some peaks and appearance of new peaks. Twelve degradation intermediates were identified by LC–MS. They were desmalachite green, didesmalachite green, tetradesmalachite green, 4-(diphenylmethyl)aniline, malachite green carbinol, bis[4-(dimethylamino)phenyl]methanone, [4-(dimethylamino)phenyl][4-(methyl-amino)phenyl]methanone, bis[4-(methylamino)phenyl]methanone, (4-amino- phenyl)[4-(methylamino)phenyl]methanone, bis(4-amino phenyl)methanone, (4-amino phenyl)methanone, and 4-(dimathylamino)benzaldehyde. According to LC–MS and FTIR data, two pathways for MG degradation by using Pseudomonas plecoglossicide MG2 were proposed. MG showed cytotoxicity to human normal retina cell line with LC50 of 28.9 µg/ml and LC90 at 79.7 µg/ml. On the other hand, MG bio-degraded products showed no toxicity to the tested cell line. Finally, this study proved that Pseudomonas plecoglossicide MG2 could be used as an efficient, renewable, eco-friendly, sustainable and cost-effective biotechnology tool for the treatment of dye wastewater effluent.
Related factors of renal injury in primary Sjögren's syndrome
Authors: Duan Nan , Li Zhiyan , Fan Yong , Jiang Yaping , Li Haixia 
Published: September 2023

Abstract
Background
Primary Sjögren’s syndrome (pSS) is a common chronic systemic autoimmune disorder which primarily affects the exocrine glands. Patients may have extraglandular disease involving multiple organs, including the kidneys. This study aimed at investigating the clinical data and laboratory markers which were associated with renal function damage or renal involvement.

Method
One thousand two hundred eighty-eight adult pSS patients from the Department of Rheumatology and Clinical Immunology were enrolled in this retrospective cohort study. And there were 334 patients of them followed up for more than two years for analyzing demographic, clinical data and laboratory markers. Statistical analysis was performed by R software (Version 3.6.2).

Result
Nearly 95% of 1288 pSS patients were women, and the positive rates of anti-SSA (Sjögren's syndrome A) and anti-SSB were 63% and 27% respectively. 12% of the pSS patients presented renal involvement with eGFR < 60 mL/min/1.73 m2, and the mean age of hospital presentation, serum creatinine and urea were the highest (P < 0.001), and ANA (antinuclear antibody)-positive, anti-SSB-positive and anti-scl-70-positive were more prevalent in this group. Multivariate analyses showed that age, urea, chlorine and anti-SSA indicate a significant association with renal dysfunction. Potassium, sodium and Jo-1 were also confirmed to be related with decreased renal function. The receiver operating characteristic (ROC) analysis including the above factors showed a good performance on the evaluation of renal injury including eGFR < 60 mL/min/1.73 m2 and eGFR 60 -90 mL/min/1.73 m2 in pSS, with area under curve (AUC) values of 0.957 and 0.821, and high sensitivity (71.1% and 84.4%) and specificity (95.5% and 70.5%). After a more than two years follow-up of anti-SSA positive patients, 34.14% of them developed decreased renal function, and 13.58% of them experienced a progression of renal injury with a 23.64% decrease in eGFR.

Conclusion
Age, urea, chlorine, and anti-SSA were highly associated with renal injury in pSS. Early screening for autoantibodies would be meaningful for evaluation and prevention of renal injury in pSS.
Microplastics removal from aqueous environment by metal organic frameworks
Authors: Honarmandrad Zhila , Kaykhaii Massoud , Gębicki Jacek 
Published: September 2023

AbstractThis paper provides an overview of recent research performed on the applications of metal–organic frameworks (MOFs) for microplastics (MPs) removal from aqueous environments. MPs pollution has become a major environmental concern due to its negative impacts on aquatic ecosystems and human health. Therefore, developing effective and sustainable methods for removing them from aqueous environments is crucial. In recent years, MOFs have emerged as a promising solution for this purpose due to their unique properties such as high surface area, renewability, chemical stability, and versatility. Moreover, their specific properties such as their pore size and chemical composition can be tailored to enhance their efficiency in removing MPs. It has been shown that MOFs can effectively adsorb MPs from aqueous media in the range of 70–99.9%. Besides some high price concerns, the main drawback of using MOFs is their powder form which can pose challenges due to their instability. This can be addressed by supporting MOFs on other substrates such as aerogels or foams. Meanwhile, there is a need for more research to investigate the long-term stability of MOFs in aqueous environments and developing efficient regeneration methods for their repeated use.
Long-read sequencing reveals the landscape of aberrant alternative splicing and novel therapeutic target in colorectal cancer
Authors: Sun Qiang , Han Ye , He Jianxing , Wang Jie , Ma Xuejie , Ning Qianqian , Zhao Qing , Jin Qian , Yang Lili , Li Shuang , Li Yang , Zhi Qiaoming , Zheng Junnian , Dong Dong 
Published: September 2023

Abstract
Background
Alternative splicing complexity plays a vital role in carcinogenesis and cancer progression. Improved understanding of novel splicing events and the underlying regulatory mechanisms may contribute new insights into developing new therapeutic strategies for colorectal cancer (CRC).

Methods
Here, we combined long-read sequencing technology with short-read RNA-seq methods to investigate the transcriptome complexity in CRC. By using experiment assays, we explored the function of newly identified splicing isoform TIMP1 Δ4-5. Moreover, a CRISPR/dCasRx-based strategy to induce the TIMP1 exon 4–5 exclusion was introduced to inhibit neoplasm growth.

Results
A total of 90,703 transcripts were identified, of which > 62% were novel compared with current transcriptome annotations. These novel transcripts were more likely to be sample specific, expressed at relatively lower levels with more exons, and oncogenes displayed a characteristic to generate more transcripts in CRC. Clinical outcome data analysis showed that 1472 differentially expressed alternative splicing events (DEAS) were tightly associated with CRC patients’ prognosis, and many novel isoforms were likely to be important determinants for patient survival. Among these, newly identified splicing isoform TIMP1 Δ4-5 was significantly downregulated in CRC. Further in vitro and in vivo assays demonstrated that ectopic expression of TIMP1 Δ4-5 significantly suppresses tumor cell growth and metastasis. Serine/arginine-rich splicing factor 1 (SRSF1) acts as a onco-splicing regulator through sustaining the inclusion of TIMP1 exon 4–5. Furthermore, CRISPR/dCasRx-based strategies designed to induce TIMP1 exon 4–5 exclusion have the potential to restrain the CRC growth.

Conclusions
This data provides a rich resource for deeper studies of gastrointestinal malignancies. Newly identified splicing isoform TIMP1 Δ4-5 plays an important role in mediating CRC progression and may be a potential therapy target in CRC.
Measurement properties of device-based physical activity instruments in ambulatory adults with physical disabilities and/or chronic diseases: a scoping review
Authors: Brandenbarg Pim , Hoekstra Femke , Barakou Ioulia , Seves Bregje L. , Hettinga Florentina J. , Hoekstra Trynke , van der Woude Lucas H. V , Dekker Rienk , Krops Leonie A. 
Published: September 2023

Abstract
Background
People with physical disabilities and/or chronic diseases tend to have an inactive lifestyle. Monitoring physical activity levels is important to provide insight on how much and what types of activities people with physical disabilities and/or chronic diseases engage in. This information can be used as input for interventions to promote a physically active lifestyle. Therefore, valid and reliable physical activity measurement instruments are needed. This scoping review aims 1) to provide a critical mapping of the existing literature and 2) directions for future research on measurement properties of device-based instruments assessing physical activity behavior in ambulant adults with physical disabilities and/or chronic diseases.

Methods
Four databases (MEDLINE, CINAHL, Web of Science, Embase) were systematically searched from 2015 to April 16th 2023 for articles investigating measurement properties of device-based instruments assessing physical activity in ambulatory adults with physical disabilities and/or chronic diseases. For the majority, screening and selection of eligible studies were done in duplicate. Extracted data were publication data, study data, study population, device, studied measurement properties and study outcome. Data were synthesized per device.

Results
One hundred three of 21566 Studies were included. 55 Consumer-grade and 23 research-grade devices were studied on measurement properties, using 14 different physical activity outcomes, in 23 different physical disabilities and/or chronic diseases. ActiGraph (n = 28) and Fitbit (n = 39) devices were most frequently studied. Steps (n = 68) was the most common used physical activity outcome. 97 studies determined validity, 11 studies reliability and 6 studies responsiveness.

Conclusion
This scoping review shows a large variability in research on measurement properties of device-based instruments in ambulatory adults with physical disabilities and/or chronic diseases. The variability highlights a need for standardization of and consensus on research in this field. The review provides directions for future research.
Use of mobile technology to identify behavioral mechanisms linked to mental health outcomes in Kenya: protocol for development and validation of a predictive model
Authors: Njoroge Willie , Maina Rachel , Frank Elena , Atwoli Lukoye , Wu Zhenke , Ngugi Anthony K , Sen Srijan , Wang JianLi , Wong Stephen , Baker Jessica A , Weinheimer-Haus Eileen M , Khakali Linda , Aballa Andrew , Orwa James , K Nyongesa Moses , Shah Jasmit , Waljee Akbar K , Abubakar Amina , Merali Zul 
Published: September 2023

Abstract
Objective
This study proposes to identify and validate weighted sensor stream signatures that predict near-term risk of a major depressive episode and future mood among healthcare workers in Kenya.

Approach
The study will deploy a mobile application (app) platform and use novel data science analytic approaches (Artificial Intelligence and Machine Learning) to identifying predictors of mental health disorders among 500 randomly sampled healthcare workers from five healthcare facilities in Nairobi, Kenya.

Expectation
This study will lay the basis for creating agile and scalable systems for rapid diagnostics that could inform precise interventions for mitigating depression and ensure a healthy, resilient healthcare workforce to develop sustainable economic growth in Kenya, East Africa, and ultimately neighboring countries in sub-Saharan Africa. This protocol paper provides an opportunity to share the planned study implementation methods and approaches.

Conclusion
A mobile technology platform that is scalable and can be used to understand and improve mental health outcomes is of critical importance.
Nematostella vectensis exemplifies the exceptional expansion and diversity of opsins in the eyeless Hexacorallia
Authors: McCulloch Kyle J. , Babonis Leslie S. , Liu Alicia , Daly Christina M. , Martindale Mark Q. , Koenig Kristen M. 
Published: September 2023

Abstract
Background
Opsins are the primary proteins responsible for light detection in animals. Cnidarians (jellyfish, sea anemones, corals) have diverse visual systems that have evolved in parallel with bilaterians (squid, flies, fish) for hundreds of millions of years. Medusozoans (e.g., jellyfish, hydroids) have evolved eyes multiple times, each time independently incorporating distinct opsin orthologs. Anthozoans (e.g., corals, sea anemones,) have diverse light-mediated behaviors and, despite being eyeless, exhibit more extensive opsin duplications than medusozoans. To better understand the evolution of photosensitivity in animals without eyes, we increased anthozoan representation in the phylogeny of animal opsins and investigated the large but poorly characterized opsin family in the sea anemone Nematostella vectensis.

Results
We analyzed genomic and transcriptomic data from 16 species of cnidarians to generate a large opsin phylogeny (708 sequences) with the largest sampling of anthozoan sequences to date. We identified 29 opsins from N. vectensis (NvOpsins) with high confidence, using transcriptomic and genomic datasets. We found that lineage-specific opsin duplications are common across Cnidaria, with anthozoan lineages exhibiting among the highest numbers of opsins in animals. To establish putative photosensory function of NvOpsins, we identified canonically conserved protein domains and amino acid sequences essential for opsin function in other animal species. We show high sequence diversity among NvOpsins at sites important for photoreception and transduction, suggesting potentially diverse functions. We further examined the spatiotemporal expression of NvOpsins and found both dynamic expression of opsins during embryonic development and sexually dimorphic opsin expression in adults.

Conclusions
These data show that lineage-specific duplication and divergence has led to expansive diversity of opsins in eyeless cnidarians, suggesting opsins from these animals may exhibit novel biochemical functions. The variable expression patterns of opsins in N. vectensis suggest opsin gene duplications allowed for a radiation of unique sensory cell types with tissue- and stage-specific functions. This diffuse network of distinct sensory cell types could be an adaptive solution for varied sensory tasks experienced in distinct life history stages in Anthozoans.
Morality in middle childhood: the role of callous-unemotional traits and emotion regulation skills
Authors: Wilke Jessica , Goagoses Naska 
Published: September 2023

Abstract
Background
The development of morality is vital for fostering prosocial behavior and enhancing both individual and societal well-being. Clarifying what contextual and individual factors play a role in moral processes during childhood can contribute to our understanding of the development of morality. Given the previous acknowledgment of importance, yet lack of existing empirical findings, the study focused on the significance of callous-unemotional traits (i.e., an affective-interpersonal personality trait, related to psychopathy in adulthood) and emotion regulation (i.e., an essential part of socio-emotional competence, and a transdiagnostic factor in the development of psychopathology) for moral emotions and cognitions during middle childhood. The concrete aim was to examine direct and indirect effects of callous-unemotional trait dimensions (callousness, uncaring, unemotionality) onto immoral emotional attribution (i.e., feeling good after immoral decisions) and admissibility of immoral actions (i.e., evaluating immoral actions as being okay) via emotion regulation skills.

Methods
A cross-sectional study was conducted with 194 children attending Grades 1 to 4, and their primary caregivers. The children completed the Inventory of Callous-Unemotional Traits and caregivers completed the Emotion Regulation Checklist. The children were also presented with a set of moral dilemma vignettes, and asked about the emotions of protagonists who acted immoral, and the admissibility of their actions.

Results
Path-model analysis revealed (1) negative direct effects of emotion regulation skills onto immoral emotional attribution and admissibility of immoral actions, (2) positive direct effects of the dimensions callousness and uncaring onto immoral emotional attribution and admissibility of immoral actions, and (3) negative direct effects of dimensions callousness and uncaring onto emotion regulation skills. Indirect effects, indicating that emotion regulation skills mediate the association between the callous-unemotional trait dimensions and morality, were also found.

Conclusion
The findings address a knowledge gap and indicate that emotion regulation skills, callousness, and uncaring play an important role in morality in middle childhood and should be included in frameworks of moral decision-making and development.
An empirical investigation of the associations between metacognition, mindfulness experiential avoidance, depression, and anxiety
Authors: Ådnøy Torstein , Solem Stian , Hagen Roger , Havnen Audun 
Published: September 2023

Abstract
Background
The aims of this study were to explore the possible relation between metacognition, mindfulness, and experiential avoidance, as well as their association with symptoms of anxiety and depression.

Methods
Cross-sectional data was collected from a community sample (N = 364) who completed the Metacognitions Questionnaire-30 (MCQ-30), the Five Facet Mindfulness Questionnaire-24 (FFMQ-24), the Acceptance and Action Questionnaire-II (AAQ-II), the Generalized Anxiety Disorder 7-item (GAD-7), and the Patient Health Questionnaire-9 (PHQ-9).

Results
There were moderate-strong associations between mindfulness (FFMQ-24), metacognition (MCQ-30), and experiential avoidance (AAQ-II) (0.62 − 0.67), and they showed similar relations with symptoms of depression (PHQ-9) and anxiety (GAD-7) (0.57 − 0.71). Metacognition, experiential avoidance, and the non-judging subscale of FFMQ-24 constituted a latent factor of flexibility in cognition and emotional experience, while three FFMQ-24 subscales (describing, acting with awareness, and observing) constituted a present-centered attention and awareness factor. Regression analyses indicated that flexibility explained more of the variance in symptoms of anxiety and depression than present-centered attention and awareness.

Conclusions
The results suggest that flexibility in cognitive and emotional regulation skills could be important in explaining symptoms of anxiety and depression.
Assessing the decorative techniques of two Art Nouveau glass windows by optical coherence tomography (OCT)
Authors: Palomar Teresa , Iwanicka Magdalena , Pombo Cardoso Isabel , Vilarigues Marcia , Targowski Piotr 
Published: September 2023

Abstract
This work presents an assessment of the different decoration techniques applied in the two glass windows from the Casa-Museu Dr. Anastácio Gonçalves (Lisbon, Portugal) by Optical coherence tomography (OCT). The Dining Room glass window was found to be decorated with grisailles and acid etching, and the Atelier window with grisaille, enamel, and cold painting. The alteration state of the surface decorations was also assessed. The thicker surface decorations (grisailles and enamels) presented cracks and detachments, in contrast to the thinner lines which are normally well preserved. OCT is a suitable technique for characterizing glass decoration in situ without dismounting the stained-glass window; and able to reveal the condition and methods of manufacture that cannot be detected simply by visual inspection.
Postseismic gravity changes after the 2011 Tohoku earthquake observed by superconducting gravimeters at Mizusawa, Japan
Authors: Tamura Yoshiaki , Kazama Takahito , Nishiyama Ryuichi , Matsuo Koji , Imanishi Yuichi 
Published: September 2023

AbstractPostseismic gravity changes after the 2011 Tohoku earthquake (Mw9.0) were investigated using the data from superconducting gravimeters (SGs) at Mizusawa, Japan. The data in the period from 2014 to 2021 were used in the analysis. The SG data were first corrected for instrumental drift using the results of absolute gravity measurements. Then, correction for the hydrological effect was applied based on physical modeling of soil moisture. Finally, the effect of vertical displacement of the station (free-air effect) was corrected using GNSS data. After these corrections, residual gravity indicated a long-term increase, with its rate gradually decreasing with time. This fact suggests that viscoelastic relaxation after the earthquake played an important role in producing the long-term gravity changes. Fitting a decaying exponential function of time to the residual series yielded 89.4 ± 4.4 µGal (1 µGal = 10–8 ms–2) as the total gravity change and 635 ± 17 days as the characteristic time scale. In addition to the ground-based observations, the data from satellite gravity missions GRACE/GRACE-FO were analyzed to retrieve gravity changes at Mizusawa. Similar analysis of the satellite-based data yielded 15.2 ± 1.6 µGal as the total gravity change and 3444 ± 599 days as the characteristic time scale. The difference in the estimates of the total gravity change, of a factor of about 6, from the ground-based and the satellite-based observations may be attributed to the limited spatial resolution in the latter method. The difference in the estimates of the time scale, of a factor of about 1/5, may originate from the difference in the depth where the two kinds of gravimetry are mainly sensitive. Referring to recent theoretical studies on postseismic deformations after the 2011 Tohoku earthquake, our results can be interpreted consistently by assuming the existence of a layer of viscoelastic materials with viscosity $$2\times {10}^{18} \text{Pa s}$$

2
×

10

18

Pa s

underneath the Tohoku area of Japan.
Graphical Abstract