bluuebunny/crossref_metadata_2025_split huggingface dataset:
Space–time self-harm and suicide clusters in two cities in Taiwan
Authors: Lu Fang-Wen , Conway Erica , Liang Ya-Lun , Chen Ying-Yeh , Gunnell David , Chang Shu-Sen 
Published: June 2023

Abstract

Aims
Suicidal acts may cluster in time and space and lead to community concerns about further imitative suicidal episodes. Although suicide clusters have been researched in previous studies, less is known about the clustering of non-fatal suicidal behaviour (self-harm). Furthermore, most previous studies used crude temporal and spatial information, e.g., numbers aggregated by month and residence area, for cluster detection analysis. This study aimed to (i) identify space–time clusters of self-harm and suicide using daily incidence data and exact address and (ii) investigate the characteristics of cluster-related suicidal acts.

Methods
Data on emergency department presentations for self-harm and suicide deaths in Taipei City and New Taipei City, Taiwan, were used in this study. In all-age and age-specific analyses, self-harm and suicide clusters were identified using space–time permutation scan statistics. A cut-off of 0.10 for the p value was used to identify possible clusters. Logistic regression was used to investigate the characteristics associated with cluster-related episodes.

Results
A total of 5,291 self-harm episodes and 1,406 suicides in Taipei City (2004–2006) and 20,531 self-harm episodes and 2,329 suicides in New Taipei City (2012–2016) were included in the analysis. In the two cities, two self-harm clusters (n [number of self-harm episodes or suicide deaths in the cluster] = 4 and 8 in Taipei City), four suicide clusters (n = 3 in Taipei City and n = 4, 11 and 4 in New Taipei City) and two self-harm and suicide combined clusters (n = 4 in Taipei City and n = 8 in New Taipei City) were identified. Space–time clusters of self-harm, suicide, and self-harm and suicide combined accounted for 0.05%, 0.59%, and 0.08% of the respective groups of suicidal acts. Cluster-related episodes of self-harm and suicide were more likely to be male (adjusted odds ratio [aOR] = 2.22, 95% confidence interval [CI] 1.26, 3.89) and young people aged 10–29 years (aOR = 2.72, 95% CI 1.43, 5.21) than their cluster-unrelated counterparts.

Conclusions
Space–time clusters of self-harm, suicide, and self-harm and suicide combined accounted for a relatively small proportion of suicidal acts and were associated with some sex/age characteristics. Focusing on suicide deaths alone may underestimate the size of some clusters and/or lead to some clusters being overlooked. Future research could consider combining self-harm and suicide data and use social connection information to investigate possible clusters of suicidal acts.
A Note on Aerosol Processing by Droplet Collision‐Coalescence
Authors: Hoffmann Fabian , Feingold Graham 
Published: June 2023

AbstractThe processing of aerosol by droplet collision‐coalescence is analyzed in three‐dimensional simulations of drizzling stratocumulus using a Lagrangian cloud model for the representation of aerosol and cloud microphysics. Collision‐coalescence processing is shown to create a characteristic bimodality in the aerosol size distribution. We show that the large‐scale dynamics of the stratocumulus‐topped boundary layer are key to understanding the amount of time available for collision‐coalescence processing. The large‐scale dynamics enable aerosol particles to repeat a cycle of droplet condensation, collision‐coalescence, and evaporation, which causes a steady increase in aerosol size. This process is continued until the aerosol grows so large that droplet growth is substantially accelerated and multiple collisions occur within one cycle, forming precipitation‐sized droplets that are lost to the surface, including the aerosol. An analytical relationship is derived that captures the fundamental shape of the processed aerosol size distribution.
Leveraging genetic overlap between irritability and psychiatric disorders to identify genetic variants of major psychiatric disorders
Authors: Jung Kyeongmin , Yoon Joohyun , Ahn Yeeun , Kim Soyeon , Shim Injeong , Ko Hyunwoong , Jung Sang-Hyuk , Kim Jaeyoung , Kim Hyejin , Lee Dong June , Cha Soojin , Lee Hyewon , Kim Beomsu , Cho Min Young , Cho Hyunbin , Kim Dan Say , Kim Jinho , Park Woong-Yang , Park Tae Hwan , O`Connell Kevin S. , Andreassen Ole A. , Myung Woojae , Won Hong-Hee 
Published: June 2023

AbstractIrritability is a heritable core mental trait associated with several psychiatric illnesses. However, the genomic basis of irritability is unclear. Therefore, this study aimed to 1) identify the genetic variants associated with irritability and investigate the associated biological pathways, genes, and tissues as well as single-nucleotide polymorphism (SNP)-based heritability; 2) explore the relationships between irritability and various traits, including psychiatric disorders; and 3) identify additional and shared genetic variants for irritability and psychiatric disorders. We conducted a genome-wide association study (GWAS) using 379,506 European samples (105,975 cases and 273,531 controls) from the UK Biobank. We utilized various post-GWAS analyses, including linkage disequilibrium score regression, the bivariate causal mixture model (MiXeR), and conditional and conjunctional false discovery rate approaches. This GWAS identified 15 independent loci associated with irritability; the total SNP heritability estimate was 4.19%. Genetic correlations with psychiatric disorders were most pronounced for major depressive disorder (MDD) and bipolar II disorder (BD II). MiXeR analysis revealed polygenic overlap with schizophrenia (SCZ), bipolar I disorder (BD I), and MDD. Conditional false discovery rate analyses identified additional loci associated with SCZ (number [n] of additional SNPs = 105), BD I (n = 54), MDD (n = 107), and irritability (n = 157). Conjunctional false discovery rate analyses identified 85, 41, and 198 shared loci between irritability and SCZ, BD I, and MDD, respectively. Multiple genetic loci were associated with irritability and three main psychiatric disorders. Given that irritability is a cross-disorder trait, these findings may help to elucidate the genomics of psychiatric disorders.
SIRT1 ubiquitination is regulated by opposing activities of APC/C-Cdh1 and AROS during stress-induced premature senescence
Authors: Lee Sang Hyup , Yang Ji-Hye , Park Ui-Hyun , Choi Hanbyeul , Kim Yoo Sung , Yoon Bo-Eun , Han Hye-Jeong , Kim Hyun-Taek , Um Soo-Jong , Kim Eun-Joo 
Published: June 2023

AbstractSIRT1, a member of the mammalian sirtuin family, is a nicotinamide adenosine dinucleotide (NAD)-dependent deacetylase with key roles in aging-related diseases and cellular senescence. However, the mechanism by which SIRT1 protein homeostasis is controlled under senescent conditions remains elusive. Here, we revealed that SIRT1 protein is significantly downregulated due to ubiquitin-mediated proteasomal degradation during stress-induced premature senescence (SIPS) and that SIRT1 physically associates with anaphase-promoting complex/cyclosome (APC/C), a multisubunit E3 ubiquitin ligase. Ubiquitin-dependent SIRT1 degradation is stimulated by the APC/C coactivator Cdh1 and not by the coactivator Cdc20. We found that Cdh1 depletion impaired the SIPS-promoted downregulation of SIRT1 expression and reduced cellular senescence, likely through SIRT1-driven p53 inactivation. In contrast, AROS, a SIRT1 activator, reversed the SIRT1 degradation induced by diverse stressors and antagonized Cdh1 function through competitive interactions with SIRT1. Furthermore, our data indicate opposite roles for Cdh1 and AROS in the epigenetic regulation of the senescence-associated secretory phenotype genes IL-6 and IL-8. Finally, we demonstrated that pinosylvin restores downregulated AROS (and SIRT1) expression levels in bleomycin-induced mouse pulmonary senescent tissue while repressing bleomycin-promoted Cdh1 expression. Overall, our study provides the first evidence of the reciprocal regulation of SIRT1 stability by APC/C-Cdh1 and AROS during stress-induced premature senescence, and our findings suggest pinosylvin as a potential senolytic agent for pulmonary fibrosis.
The effectiveness of global protected areas for climate change mitigation
Authors: Duncanson L. , Liang M. , Leitold V. , Armston J. , Krishna Moorthy S. M. , Dubayah R. , Costedoat S. , Enquist B. J. , Fatoyinbo L. , Goetz S. J. , Gonzalez-Roglich M. , Merow C. , Roehrdanz P. R. , Tabor K. , Zvoleff A. 
Published: June 2023

AbstractForests play a critical role in stabilizing Earth’s climate. Establishing protected areas (PAs) represents one approach to forest conservation, but PAs were rarely created to mitigate climate change. The global impact of PAs on the carbon cycle has not previously been quantified due to a lack of accurate global-scale carbon stock maps. Here we used ~412 million lidar samples from NASA’s GEDI mission to estimate a total PA aboveground carbon (C) stock of 61.43 Gt (+/− 0.31), 26% of all mapped terrestrial woody C. Of this total, 9.65 + /− 0.88 Gt of additional carbon was attributed to PA status. These higher C stocks are primarily from avoided emissions from deforestation and degradation in PAs compared to unprotected forests. This total is roughly equivalent to one year of annual global fossil fuel emissions. These results underscore the importance of conservation of high biomass forests for avoiding carbon emissions and preserving future sequestration.
Identification of a covert evolutionary pathway between two protein folds
Authors: Chakravarty Devlina , Sreenivasan Shwetha , Swint-Kruse Liskin , Porter Lauren L. 
Published: June 2023

AbstractAlthough homologous protein sequences are expected to adopt similar structures, some amino acid substitutions can interconvert α-helices and β-sheets. Such fold switching may have occurred over evolutionary history, but supporting evidence has been limited by the: (1) abundance and diversity of sequenced genes, (2) quantity of experimentally determined protein structures, and (3) assumptions underlying the statistical methods used to infer homology. Here, we overcome these barriers by applying multiple statistical methods to a family of ~600,000 bacterial response regulator proteins. We find that their homologous DNA-binding subunits assume divergent structures: helix-turn-helix versus α-helix + β-sheet (winged helix). Phylogenetic analyses, ancestral sequence reconstruction, and AlphaFold2 models indicate that amino acid substitutions facilitated a switch from helix-turn-helix into winged helix. This structural transformation likely expanded DNA-binding specificity. Our approach uncovers an evolutionary pathway between two protein folds and provides a methodology to identify secondary structure switching in other protein families.
Structural interplay of anesthetics and paralytics on muscle nicotinic receptors
Authors: Goswami Umang , Rahman Md Mahfuzur , Teng Jinfeng , Hibbs Ryan E. 
Published: June 2023

AbstractGeneral anesthetics and neuromuscular blockers are used together during surgery to stabilize patients in an unconscious state. Anesthetics act mainly by potentiating inhibitory ion channels and inhibiting excitatory ion channels, with the net effect of dampening nervous system excitability. Neuromuscular blockers act by antagonizing nicotinic acetylcholine receptors at the motor endplate; these excitatory ligand-gated ion channels are also inhibited by general anesthetics. The mechanisms by which anesthetics and neuromuscular blockers inhibit nicotinic receptors are poorly understood but underlie safe and effective surgeries. Here we took a direct structural approach to define how a commonly used anesthetic and two neuromuscular blockers act on a muscle-type nicotinic receptor. We discover that the intravenous anesthetic etomidate binds at an intrasubunit site in the transmembrane domain and stabilizes a non-conducting, desensitized-like state of the channel. The depolarizing neuromuscular blocker succinylcholine also stabilizes a desensitized channel but does so through binding to the classical neurotransmitter site. Rocuronium binds in this same neurotransmitter site but locks the receptor in a resting, non-conducting state. Together, this study reveals a structural mechanism for how general anesthetics work on excitatory nicotinic receptors and further rationalizes clinical observations in how general anesthetics and neuromuscular blockers interact.
3D modelling and simulation of the impact of wearing a mask on the dispersion of particles carrying the SARS-CoV-2 virus in a railway transport coach
Authors: Armand Patrick , Tâche Jérémie 
Published: June 2023

AbstractEven though the Covid-19 pandemic seems to be stagnating or decreasing across the world, a resurgence of the disease or the occurrence of other epidemics caused by the aerial dissemination of pathogenic biological agents cannot be ruled out. These agents, in particular the virions of the Covid-19 disease, are found in the particles originating from the sputum of infected symptomatic or asymptomatic people. In previous research, we made use of a three-dimensional Computational Fluid Dynamics (CFD) model to simulate particle transport and dispersion in ventilated semi-confined spaces. By way of illustration, we considered a commuter train coach in which an infected passenger emitted droplets (1 and 10 µm) and drops (100 and 1000 µm) while breathing and coughing. Using an Eulerian approach and a Lagrangian approach, we modelled the dispersion of the particles in the turbulent flow generated by the ventilation of the coach. The simulations returned similar results from both approaches and clearly demonstrated the very distinct aerodynamics of the aerosol of airborne droplets and, at the other end of the spectrum, of drops falling or behaving like projectiles depending on their initial velocity. That numerical study considered passengers without protective masks. In this new phase of research, we first used literature data to develop a model of a typical surgical mask for use on a digital manikin representing a human. Next, we resumed the twin experiment of the railway coach, but this time, the passengers (including the infected one) were provided with surgical masks. We compared the spatial and temporal distributions of the particles depending on whether the spreader passenger wore a mask at all, and whether the mask was perfectly fitted (without leaks) or worn loosely (with leaks). Beyond demonstrating the obvious value of wearing a mask in limiting the dissemination of particles, our model and our simulations allow a quantification of the ratio of particles suspended in the coach depending on whether the infected passenger wears a mask or not. Moreover, the calculations carried out constitute only one illustrative application among many others, not only in public transport, but in any other public or private ventilated space on the basis of the same physical models and digital twins of the places considered. CFD therefore makes it possible to estimate the criticality of the occupation of places by people with or without a mask and to recommend measures in order to limit aerial contamination by any kind of airborne pathogen, such as the virions of Covid-19.
On the thermodynamics of DNA methylation process
Authors: Sanchez Robersy , Mackenzie Sally A. 
Published: June 2023

AbstractDNA methylation is an epigenetic mechanism that plays important roles in various biological processes including transcriptional and post-transcriptional regulation, genomic imprinting, aging, and stress response to environmental changes and disease. Consistent with thermodynamic principles acting within living systems and the application of maximum entropy principle, we propose a theoretical framework to understand and decode the DNA methylation process. A central tenet of this argument is that the probability density function of DNA methylation information-divergence summarizes the statistical biophysics underlying spontaneous methylation background and implicitly bears on the channel capacity of molecular machines conforming to Shannon’s capacity theorem. On this theoretical basis, contributions from the molecular machine (enzyme) logical operations to Gibb entropy (S) and Helmholtz free energy (F) are intrinsic. Application to the estimations of S on datasets from Arabidopsis thaliana suggests that, as a thermodynamic state variable, individual methylome entropy is completely determined by the current state of the system, which in biological terms translates to a correspondence between estimated entropy values and observable phenotypic state. In patients with different types of cancer, results suggest that a significant information loss occurs in the transition from differentiated (healthy) tissues to cancer cells. This type of analysis may have important implications for early-stage diagnostics. The analysis of entropy fluctuations on experimental datasets revealed existence of restrictions on the magnitude of genome-wide methylation changes originating by organismal response to environmental changes. Only dysfunctional stages observed in the Arabidopsis mutant met1 and in cancer cells do not conform to these rules.
The effect of the COVID-19 pandemic on life expectancy in 27 countries
Authors: Huang Guogui , Guo Fei , Zimmermann Klaus F. , Liu Lihua , Taksa Lucy , Cheng Zhiming , Tani Massimiliano , Franklin Marika 
Published: June 2023

AbstractThe expected year-on-year intrinsic mortality variations/changes are largely overlooked in the existing research when estimating the effect of the COVID-19 pandemic on mortality patterns. To fill this gap, this study provides a new assessment of the loss of life expectancy caused by COVID-19 in 27 countries considering both the actual and the expected changes in life expectancy between 2019 and 2020. Life expectancy in 2020 and the expected life expectancy in the absence of COVID-19 are estimated using the Lee-Carter model and data primarily from the Human Mortality Database. The results show that life expectancy in 21 of the 27 countries was expected to increase in 2020 had COVID-19 not occurred. By considering the expected mortality changes between 2019 and 2020, the study shows that, on average, the loss of life expectancy among the 27 countries in 2020 amounted to 1.33 year (95% CI 1.29–1.37) at age 15 and 0.91 years (95% CI 0.88–0.94) at age 65. Our results suggest that if the year-on-year intrinsic variations/changes in mortality were considered, the effects of COVID-19 on mortality are more profound than previously understood. This is particularly prominent for countries experiencing greater life expectancy increase in recent years.
Protocol development to further differentiate and transition stem cell-derived pancreatic progenitors from a monolayer into endocrine cells in suspension culture
Authors: Braam Mitchell J. S. , Zhao Jia , Liang Shenghui , Ida Shogo , Kloostra Nick K. , Iworima Diepiriye G. , Tang Mei , Baker Robert K. , Quiskamp Nina , Piret James M. , Kieffer Timothy J. 
Published: June 2023

AbstractThe generation of functional β-cells from human pluripotent stem cells (hPSCs) for cell replacement therapy and disease modeling of diabetes is being investigated by many groups. We have developed a protocol to harvest and aggregate hPSC-derived pancreatic progenitors generated using a commercially available kit into near uniform spheroids and to further differentiate the cells toward an endocrine cell fate in suspension culture. Using a static suspension culture platform, we could generate a high percentage of insulin-expressing, glucose-responsive cells. We identified FGF7 as a soluble factor promoting aggregate survival with no inhibitory effect on endocrine gene expression. Notch inhibition of pancreatic progenitor cells during aggregation improved endocrine cell induction in vitro and improved graft function following implantation and further differentiation in mice. Thus we provide an approach to promote endocrine formation from kit-derived pancreatic progenitors, either through extended culture or post implant.
Clinicopathological and prognostic significance of LKB1 expression in gastric cancer: a systematic review and meta-analysis
Authors: Tan Guojiang , Liu Baiying 
Published: June 2023

AbstractMany studies report Liver kinase B1 (LKB1) plays a critical role in gastric cancer (GC). However, the relationship between LKB1 and the clinicopathological parameters of GC patients remains controversial. This meta-analysis aimed to investigate the above question and re-evaluate the prognostic significance of LKB1 in GC patients. We searched PubMed, Web of Science, Cochrane Library, Google Scholar, CNKI, and Wan Fang to identify relevant studies published before April 20, 2023. After careful screening, 11 studies involving 1767 patients were included. We found that LKB1 expression was significantly related to tumor size (OR 0.515; 95% CI 0.316–0.839; P < 0.01), differentiation (OR 0.643; 95% CI 0.521–0.794; P < 0.001), depth of invasion (OR 0.397; 95% CI 0.319–0.494; P < 0.001), lymph node metastasis (OR 0.487; 95% CI 0.397–0.598; P = 0.01), and TNM stage (OR 0.362; 95% CI 0.293–0.447; P = 0.006). However, LKB1 was unrelated to gender and age (P > 0.05). Moreover, low LKB1 expression was significant correlate with overall survival (OS) (HR = 1.59; 95% CI 1.29–1.96; P < 0.001). In conclusion, LKB1 expression is related to tumor size, differentiation, depth of invasion, lymph node metastasis, and TNM stage, and low LKB1 expression can predict a poor prognosis. LKB1 is a potentially valuable prognosis signature and therapeutic target in GC patients.
Construction of Fe-doped ZIF-8/DOX nanocomposites for ferroptosis strategy in the treatment of breast cancer
Authors: Zhong Yuyu , Peng Zhaoxi , Peng Yanqiong , Li Bo , Pan Ying , Ouyang Qin , Sakiyama Hiroshi , Muddassir Mohd. , Liu Jianqiang 
Published: June 2023

The DOX@7FZ was able to enhance the ROS level in cancer cells via a synergistic effect between apoptosis/ferroptosis synergism.
Associations of Dietary Intake With Self-Reported Hearing Loss: Findings From the Survey of the Health of Wisconsin
Authors: Dillard Lauren K. , Nelson-Bakkum Erin , Schultz Amy , Merten Natascha , Malecki Kristen 
Published: June 2023

Purpose:
The purpose of this study was to evaluate associations of dietary intake components with hearing loss.

Method:

Participants were from the population-based Survey of the Health of Wisconsin. The Block food frequency questionnaire measured dietary intake of carbohydrates, fiber, protein, free (added) sugars, fruits, vegetables, saturated and trans fats, and glycemic index. Intake was categorized into quintiles (Q). Hearing loss was self-reported. Logistic regression models were used to evaluate associations of dietary determinants with hearing loss. Results are presented as odds ratios (
OR
s) with corresponding 95% confidence intervals (95% CIs). Final models were adjusted for age, sex, total energy intake, race/ethnicity, education, smoking, and regular physical activity.

Results:

There were 2,839 participants (56% women;
M
age
= 48.2 [
SD
= 14.5] years) included. Higher consumption of trans fat (Q5:
OR
= 1.83, 95% CI [1.27, 2.64]) and higher glycemic index (Q5:
OR
= 1.34, 95% CI [1.00, 1.80]) were associated with increased odds of hearing loss. Hearing loss was associated with fruit, saturated- and trans-fat intake in women, and trans-fat intake and glycemic index in men.

Conclusions:
Dietary intake was associated with self-reported hearing loss. Research on mechanistic pathways of associations and public health interventions to prevent hearing loss is needed.