bluuebunny/crossref_metadata_2025_split huggingface dataset:
Standardized Residue Numbering and Secondary Structure Nomenclature in the Class D β-Lactamases
Authors: Stasyuk Anastasiya , Smith Clyde A. 
Published: January 2025

AbstractOver 1370 class D β-lactamases are currently known, and they pose a serious threat to the effective treatment of many infectious diseases, particularly in some pathogenic bacteria where evolving carbapenemase activity has been reported. Detailed understanding of their molecular biology, enzymology and structural biology are critically important but the lack of a standardized residue numbering scheme and inconsistent secondary structure annotation has made comparative analyses sometimes difficult and cumbersome. Compounding this, in the post-AlphaFold world where we currently find ourselves, an extraordinary wealth of detailed structural information on these enzymes is literally at our fingertips, therefore it is vitally important that a standard numbering system is in place to facilitate the accurate and straightforward analysis of their structures. Here we present a residue numbering and secondary structure scheme for the class D enzymes and apply it to test targets to demonstrate ease at which it can be used.
GAL-101 prevents amyloid beta-induced membrane depolarization in two different types of retinal cells
Authors: Pizzi Erika , Gornati Simona , Stabilini Stefano , Brambilla Dario , Mercurio Chiara A. , Russ Hermann , Parsons Christopher G. , Mazzanti Michele 
Published: January 2025

AbstractGlaucoma and age-related macular degeneration (AMD) are two of the major causes of progressive vision loss and ultimately blindness worldwide. Both retinopathies share several pathological features with Alzheimer’s disease (AD) such as: impairment of neuronal function, astrocytosis, and activation of immune-competent microglia and Müller cells. It also has been shown that these conditions are characterized by the presence of an elevated concentration of amyloid beta (Aβ). Under pathological conditions, Aβ1-42tends to aggregate, forming toxic soluble oligomers, considered to be the most harmful amyloid species. One strategy adopted to prevent cell damage caused by these oligomers is to impair their aggregation. Here we studied GAL-101, a small molecule designed to modify the aggregation of Aβ1-42.To assess the role of GAL-101 in the aggregation of Aβ1-42,in vitroelectrophysiological measurements on retinal ganglion cells (RGCs) and retinal pigment epithelial (RPE) cells were performed to determine the polarization of the resting membrane potential. Cells treated only with Aβ1-42oligomers showed a strong depolarization of the resting membrane potential, which is believed to be the main reason for retinal cells malfunctioning in neurodegenerative diseases of the eye. Pre-incubation with GAL-101 stabilized the cell resting potential to around -50mV during exposure to Aβ1-42, in both RGCs and RPE cells. GAL-101 was able to prevent changes in resting membrane potential and thus would be expected to prevent impairment of retinal cell function. These results are supportive of evaluating GAL-101 as a potential treatment of Aβ-associated retinopathies like glaucoma and dry AMD.
Estimating the motor exploration in reinforcement learning
Authors: Zai Anja T. , Lorenz Corinna , Srikantharajah Shakana , Giret Nicolas , Hahnloser Richard H.R. 
Published: January 2025

AbstractWhat motor exploration strategies do animals use to learn a skill from rewards? Reinforcement learning theory provides no guidance for estimating motor exploration — the behavioral component aimed at discovering better strategies. Inspired by the brain’s modular organization, we postulate a latent learner that explores via an additive source of ideal randomness it injects into behavior.Assuming the learner is ignorant of other motor components, which is sub-optimal by design, evolutionary fitness argues that these should display mainly non-ideal variability.We test this recipe for behavior decomposition in songbirds subjected to a vocal pitch conditioning task. The explorative component we estimate from vocalizations accounts for the motor contribution of a basal ganglia pathway and the other behavioral component accounts for birds’ suboptimal learning trajectories. This congruence between normative exploration and brain organization suggests that the evolutionary pressure for behavioral optimality is lesser than to learn from purely random trials.
p16.1andp16.2, new HSPC markers, play redundant roles in zebrafish T-cell lymphopoiesis
Authors: Gomez Etienne , Li Roman A. , Bertrand Julien Y. 
Published: January 2025

AbstractIn the recent years, the zebrafish model has become a first-choice animal model in the field of hematopoiesis, due to extra-uterine development and optical transparency that allow an easy observation of early stages of hematopoietic stem and progenitor cells (HSPCs) development. Here, we characterized the function of two undescribed genes,si:ch211-214p16.1andsi:ch211-214p16.2(shortenedp16.1andp16.2) that are expressed by HSPCs, as early as their emergence. By combining different strategies, we have demonstrated thatp16.1andp16.2play redundant roles in embryonic thymopoiesis. Knocking-down both genes at the same time – or one gene in the mutant background for the other gene – impaired HSPCs commitment towards lymphoid fate and homing to the thymus. However double mutant embryos did not show any phenotype. As these double-mutant animals were viable, their thymocytes were compared to their wild-type counterparts by RNA sequencing, revealing that double mutant thymocytes compensated the lack ofp16.1andp16.2by upregulating genes involved in chemotaxis and cell migration. One of these transcripts,ccr9b, was indeed validated as an important regulator of thymic homing in double mutant embryos, only. In summary, we found two new markers of embryonic HSPCs, functionally linked to lymphoid fate. The description of such markers will be important for our understanding of heterogeneity among emerging HSPCs in the embryo.
Who is taking climate action in university? Drivers of personal and professional climate action in higher education
Authors: Ferrari Enzo , Whitmarsh Lorraine , Haggar Paul , Mitev Kaloyan , Lowe Alice 
Published: January 2025

Purpose
Climate change (CC) poses significant risks to society, but there are ways people can address it – including in their personal and professional lives. One professional context – higher education – has a unique role in tackling CC through educating future leaders and researching potential solutions. This study aims to identify the predictors that determine climate action in the university.

Design/methodology/approach
The predictors of climate action (including both personal behaviour change and academic subject choice) are examined amongst both university students and staff at a UK university. The authors present the results of an online survey (N = 3,326).

Findings
Climate education and research were associated with early and mid-career researchers, years working/studying and academic field, with engineering staff/students most involved. Climate anxiety and awareness of university climate emergency declarations and credible climate information sources significantly explain academic behaviour among students and academics. In addition, activities with substantial carbon footprints, such as driving and eating ruminant meat, could be associated with CC research and teaching.

Originality/value
These results highlight the importance of improving climate literacy, and sustainability initiatives within higher education. To address the urgent issues of CC, higher education institutions must integrate climate education, research and sustainable practices.
Beyond aesthetics: investigating the interplay between office design, employer brand and employee intention to leave
Authors: de Campos Ribeiro Gisele , Minchella Delphine 
Published: January 2025

Purpose
Through the perspective of current employees, this study aims to investigate the relationship between different office configurations, employer brand evaluations and employees’ intentions to leave the company.

Design/methodology/approach
This paper studied 2,025 office employees in the United Kingdom, France, Germany and Italy using an online survey. It employed ordinary least squares (OLS) regression and mediation analysis to investigate how office type relates to employer brand evaluations and the intention to leave the company.

Findings
The analysis shows a significant relationship between office type and both variables. Three office configurations shape these relationships: shared rooms and flex offices lower employer brand evaluations, while large open-plan offices increase turnover intention. Employer brand mediates the relationship between office type and intention to leave the company. Shared rooms and flex spaces indirectly raise turnover intention by lowering employer brand ratings.

Originality/value
To the best of the authors’ knowledge, this study is among the first to examine the interplay between different office types, employer brand evaluations and employees’ intentions to leave the company from the perspective of current employees.
What incentivises innovative firms to avoid taxes?
Authors: Toumi Fadoua , Jouini Fathi 
Published: January 2025

Purpose
Prior studies have examined the relationship between tax avoidance and corporate innovation, showing that innovative firms use their intellectual properties and research and development (R&D) activities to avoid taxes. This study aims to investigate the motivation behind tax avoidance practices within innovative firms.

Design/methodology/approach
This study uses the Compustat database to collect financial information for US companies, comprising 33,872 firm-year observations from 5,504 non-financial from 2011 to 2021. To ensure robust findings, the authors conducted the Hussman test to compare fixed effects and random effects models, the Breusch–Pagan test for heteroskedasticity and the Koenker and Machado test to assess coefficient consistency across quantiles in quantile regressions (QRs). Based on these tests, the authors used two empirical approaches: a random-effect model to examine the linear relationship between variables and QR to capture potential non-linear relationships and address heteroskedasticity issues.

Findings
Results indicate that corporate patent activity facilitates tax avoidance practices; however, not all innovative firms use their intellectual properties (e.g. patents) for this purpose. The association between tax avoidance and innovation is negative at the lowest quantile, suggesting that high innovation output and quality patents can enhance tax avoidance. This is likely because high-quality patents are valuable and generate more profit, increasing income shifted to foreign subsidiaries. In addition, the study found that innovative firms investing more in R&D benefit from tax advantages and pay less taxes than non-innovative firms. Finally, testing for the moderating effect of foreign income showed no impact on the relationship between tax avoidance and corporate innovation. The results are robust for alternative testing and measuring change.

Originality/value
To the best of the authors’ knowledge, this is one of the first studies to investigate the incentives that drive innovative firms to avoid taxes.
What role does the “smart employee” play in the economic development of the tourism sector. An empirical study in Spanish hotels
Authors: Folgado-Fernández José A. , Robina-Ramirez Rafael , Pizarro-Polo Ángel , Arenas Escaso Juan Francisco 
Published: January 2025

Purpose
This study aims to define the “smart tourism employee” and explore how combining technological and interpersonal skills helps tourism workers adapt to emerging technologies.

Design/methodology/approach
A qualitative-quantitative methodology was used, proposing 22 indicators related to defining the “smart tourism employee.”

Findings
Analysis of 883 responses from hotel employees across Spain using SmartPLS 4 revealed the crucial role of both technological and interpersonal skills and the importance of periodic evaluation aligned with hotels’ innovation orientation.

Practical implications
The study highlights that enhancing hotel competitiveness through technology requires employees who can effectively use the latest technological advances for customers.

Originality/value
Unlike prior research focusing solely on technological aspects in the tourism sector, this study emphasizes the need for comprehensive training of employees in technology use.
Hybrid HRM systems and employee well-being: a self-determination theory perspective
Authors: Liu Li , She Long , Cafferkey Kenneth , Townsend Keith 
Published: January 2025

PurposeDrawing from the human resource management (HRM) literature and framed with self-determination theory, this article seeks to investigate the impact of a hybrid system of HRM, characterized by the integration of practices from high-involvement work systems (HIWS) and high-compliance work systems (HCWS), on employee well-being in China.Design/methodology/approachWe hypothesize there is a positive relationship between the hybrid system and employee well-being, mediated by the satisfaction of basic psychological needs as proposed by self-determination theory. To test these hypotheses, we conducted a cross-sectional survey involving 337 employees from both an agricultural company and a bank in China.FindingsOur dataset provides support for the hypotheses, indicating that hybrid human resource (HR) systems positively relate to employee well-being, with a positive association with work engagement and a negative association with emotional exhaustion. Furthermore, our findings reveal that this relationship is mediated by the satisfaction of basic psychological needs.Originality/valueThis paper contributes to the HRM literature in three significant ways. First, it explores the hybrid HRM system, which integrates high-involvement work systems (HIWS) and high-compliance work systems (HCWS), enhancing the theorization of HRM in a more comprehensive manner. Second, it utilizes self-determination theory (SDT) to illuminate the underlying mechanisms connecting hybrid HRM to employee well-being – a topic that has received limited attention in prior research. Lastly, we operationalize employee well-being by examining both work engagement and emotional exhaustion, thereby offering a comprehensive understanding of its multidimensional nature.
Examining the impact of stakeholders' pressures on sustainability practices
Authors: Sun Guo , Sulemana Inusah , Agyemang Andrew Osei 
Published: January 2025

PurposeThe study aims to investigate the influence of stakeholder pressure on sustainability practices via the mediating effect of green innovation (GI) from a developing economy in Sub-Saharan Africa (SSA).Design/methodology/approachThe study used primary data from 567 respondents in manufacturing and mining firms in Ghana to conduct the Partial Least Squares Structural Equation Modeling (PLS-SEM) analysis using Smart-PLS, Version 4.0. Data was gathered using survey questionnaire. A purposive sampling method was used to choose participants' companies.FindingsThe findings revealed that shareholder and government pressure significantly and positively influence sustainability practices, while pressure from the community has an insignificant beneficial impact on sustainability practices. Consumer pressure revealed an insignificant detrimental influence on sustainability practices. The study discovered that green innovation fully and substantially mediates the association between stakeholder pressure and sustainability practices.Research limitations/implicationsThe study has limitations, including the exclusion of control variables and its focus on four different stakeholders, excluding other stakeholders that who also impact sustainability practices.Practical implicationsThe study’s findings can assist Ghanaian companies in making strategic decisions to enhance corporate reputation, acknowledging the effect of stakeholder pressure on sustainability practices.Social implicationsThe study outcome may enable companies to implement more inclusive sustainable development initiatives, benefiting their bottom line while contributing to the well-being of local communities and the environment.Originality/valueThe study’s originality stems from integrating green innovation in examining stakeholders' influence and sustainability practices of firms, a gap that remained unexplored. It highlights the essence of green innovation in shaping a company’s sustainability practices, highlighting the need for businesses to integrate technology and sustainability in their operations.
A Text Analysis of News Media Framing of Government Response to the 2023 Türkiye–Syria Earthquake
Authors: Binici Simal , Choi Jeongho , Mitchell Sara McLaughlin , Pizzi Elise 
Published: January 2025

ABSTRACTThe February 2023 Türkiye–Syria Earthquake had a profound impact on political, economic, and social life in Türkiye. Despite criticism about government disaster response, the government of Türkiye won an election 3 months later. We examine variance in media coverage of government disaster responses with Structural Topic Modeling of 12,268 articles in five pro‐ and anti‐government newspapers in Türkiye (Anadolu Ajansi, Hurriyet, Sozcu, Cumhuriyet, and Posta) between the February earthquake and the May election. We identify five general topical themes in the data: emotion, science, information provision, government response, and the economy. We conduct sentiment analysis and find little direct criticism of the government's responses, although anti‐government newspapers are more negative on topics related to building structures and damage. The general pattern of positive sentiment among all newspapers regarding government responses to the earthquake may help us understand why the disaster was not as consequential for the election outcome as some observers expected.
Analysis of related factors for systemic lupus erythematosus flare after SARS‐Cov‐2 infection: A retrospective cohort study
Authors: Li Qian , Zhao Jun‐Kang , Zhang Jun‐Yan , Li Rong , Zhao Li , Su Ya‐Zhen , Liu Yang , Xu Ke , Zhang Li‐Yun 
Published: January 2025

AbstractObjectiveSince COVID‐19 infections are more common in systemic lupus erythematosus (SLE) patients, most recent research has focused on the outcome of COVID‐19, with fewer studies on disease activity in SLE. This research aims to evaluate flares in SLE with COVID‐19 infection while investigating predictive factors.MethodsA questionnaire survey was conducted to collect information on patients with previously diagnosed SLE from multi‐center. SLE patients infected with COVID‐19 after December 7, 2022, were selected. Detailed information covering demographic characteristics, SLE and COVID‐19 clinical features, disease activity, and medication was collected through an electronic questionnaire. A multivariate logistic regression model was constructed to evaluate the predictive factors for SLE disease onset after COVID‐19 infection.ResultsA total of 240 patients were finally included in our analysis. Thirty (12.5%) of those enrolled reported an SLE flare. Multivariate analysis with logistic models confirmed that SLE in the active stage (OR 2.617, 95% CI 1.008–6.514, p = .041) and COVID‐19 duration (OR 4.140, 95% CI 1.412–11.694, p = .008) were predictors for flare in SLE patients with Covid‐19 infection. In contrast, immunosuppressants were associated with a low incidence of flare of SLE (OR 0.138, 95% CI 0.042–0.46, p = .005).ConclusionsThe active phase of SLE and the progression of COVID‐19 were the main risk factors for disease exacerbation in SLE patients, while the use of immunosuppressive medications was associated with a lower risk of flare‐ups. These findings provide valuable insights for managing SLE patients during the COVID‐19 pandemic.
iTRAQ proteomic analysis of the anterior insula in morphine‐induced conditioned place preference rats with high‐frequency deep brain stimulation intervention
Authors: Chang Haigang , Wang Yaxiao , Hui Lei , Diao Yuling , Ma Pengju , Li Xiangsheng , Wang Feng 
Published: January 2025

AbstractMorphine dependence or addiction is a serious global public health and social problem, and traditional treatments are very limited. Deep brain stimulation (DBS) has emerged as a new potential treatment for drug addiction. Repeated use of morphine leads to neuroadaptive and molecular changes in the addiction‐related brain regions. We have previously performed isobaric tags for relative and absolute quantitation (iTRAQ) labelling coupled with 2D‐LC MS/MS in anterior insular samples from rats treated with saline control, morphine or morphine plus DBS, and the identified expression of eight proteins are altered by morphine and reversed by high‐frequency DBS (HF‐DBS). In this study, we analysed the proteomic data in more details. A total of 5575 proteins were identified. Relative to the saline group, the morphine group showed 14 down‐regulated and three up‐regulated proteins. There were 118 proteins increased and 87 proteins decreased between DBS implanted animals and morphine group. Several differentially expressed proteins were verified with parallel reaction monitoring (PRM) assay. Based on Gene Ontology enrichment an KEGG pathway analyses, the majority of these differentially expressed proteins (DEPs) were involved in protein metabolic process, G‐protein coupled receptor signalling pathway, calcium‐mediated signalling, neurotransmitter transport, dopaminergic synapse and mTOR signalling pathway. These data offer a comprehensive understanding of the proteomic changes associated with morphine addiction and DBS therapy in addicted animal models, which is important for the development of DBS interventions for drug addiction.
Unveiling Rainfall's Role in Shaping Woody Plant Functional Traits and Diversity in Northern Namibia
Authors: Shikongo Albertina M. N. , Fabiano Ezequiel C. , Fernandes Geraldo W. 
Published: January 2025

ABSTRACTChanges in precipitation patterns affect tree populations by altering their functional traits, yet functional diversity is often underestimated in ecological studies. This study analysed changes in plant species diversity and functional traits along an increasing rainfall gradient, using systematic sampling at 13 points along a 1200‐km transect. Plant species diversity fluctuated with no apparent significant effect that could be attributed to increasing rainfall gradient, yet species diversity was significantly highest at sites with high rainfall. While there was no apparent statistical difference in the Simpson Diversity Index among sites, beta diversity was high among all sites. Functional traits varied along the gradient, with functional richness significantly affected by annual average rainfall. The study underscores the importance of both plant species and functional diversity in ecosystems and suggests future research should include more functional traits and sufficient data collection to further elucidate the complex interactions between tree populations, functional traits and precipitation patterns.