bluuebunny/crossref_metadata_2025_split huggingface dataset:
Tranformasi Digital dan Ancaman Cybercrime
Authors: Rolando Dede Mercy , Aulia Hanna Hilyati , Andini Mutia Tanseba 
Published: September 2023

Jurnal ini bertujuan untuk mengetahui ke-efektifitas-an Undang-Undang ITE atas terjadinya transformasi digital yang menjadi ancaman cybercrime di Indonesia. Banyak hal buruk dan tidak di inginkan terjadi melalui teknologi informasi. Sebab itu pemerintah merasa bahwa teknologi informasi tidak hanya perlu diperhatikan, tetapi juga perlu diatur dalam hukum melalui penerapan tindak pidana Undang-Undang ITE. Namun, hanya sedikit pasal yang mengatur demi terciptanya kelancaran perdagangan secara elektronik, seperti aturan-aturan yang harus dipatuhi bagi pelaku perdagangan elektronik. Isi dari UU ITE yang paling krusial adalah justru menekankan pada perbuatan-perbuatan yang tidak bersinggungan langsung dengan perdagangan elektronik, seperti pasal 27 ayat 1 dan 3, pasal 28 ayat 2, dan pasal 29 yang cenderung menekankan masalah sosial seperti asusila, perjudian, penghinaan, pemerasan, berita bohong dan menyesatkan, berita kebencian dan permusuhan, ancaman kekerasan dan menakut-nakuti.masalah tersebut jauh dari tujuan pasal 4 ayat 2 yang telah disebutkan diatas. Ada beberapa hal yang terlewatkan yang sebenarnya harus lebih difokuskan dalam UU ITE, antara lain masalah Spamming, baik untuk email spamming maupun masalah penjualan data pribadi oleh perbankan, asuransi, Virus, worm komputer (masih implisit di Pasal 33) terutama untuk pengembangan dan penyebarannya, dan jenis-jenis phising seperti typo pirates yang membuat tidak nyaman proses perdagangan elektronik. Masalah-masalah tersebut adalah juga krusial yang seharusnya secara eksplisit diatur dalam UU ITE, karena menyangkut juga demi terciptanya kelancaran transaksi elektronik khususnya perdagangan elektronik.
On the Question of the Attitude to Sports Practices in Orthodoxy
Authors: Gonashvili A. S. 
Published: September 2023

Introduction. The article is aimed at a comprehensive and holistic analysis of the judgments existing in Orthodox circles regarding physical culture and sports, reflecting the Orthodox worldview and its values. The novelty of the article lies in the empirical substantiation of the role of the physical and spiritual development of believers against the background of sports practices, as well as the substantiation of contradictions and problems of perception, sports practices in the conditions of the Orthodox worldview. Methodology and sources. A comprehensive approach to the problem of studying and analyzing sports practices is used as a methodological basis. The main research method in the work is an Internet survey.Results and discussion. According to the study, it should be noted that there is no unity among Orthodox people in understanding the role of physical culture in Orthodox spiritual development. Most people believe that the call for the development of their body is not the work of the church, they do not see any sin in the underdevelopment of their body. This is the result of the influence of various values that are in one or another society in which people live. Therefore, it is especially important to emphasize the role of the church in the educational process of physical culture among young people and the younger generation.Conclusion. In general, the author of the article comes to the conclusion that there is no unity among Orthodox people in understanding the role of physical culture in Orthodox spiritual development.
Features of the course of pulmonary tuberculosis against the background of coronavirus infection according to computed tomography of the chest organs
Authors: Lynnyk М.І. , Іgnatieva V.І. , Gumeniuk G.L. , Svyatnenko V.А. , Ivashchenko V.Ye. , Chobotar О.P. , Palivoda М.G. 
Published: September 2023

BACKGROUND. Quarantine measures regarding the coronavirus disease (COVID-19) pandemic, initiated in early 2020, and subsequently large-scale hostilities in Ukraine, led to a decrease in the detection of tuberculosis (TB) patients and an increase in mortality from this disease. Particular attention needs to be paid to the peculiarities of the course of pulmonary TB against the background of a coronavirus infection, which may be due to both the features of the impact of SARS-CoV-2 and the features of the immunological status of patients with pulmonary TB with preserved sensitivity to antimycobacterial drugs (DSTB) and with drug resistance.
OBJECTIVE. To investigate the dynamics of the course of pathological changes in the lungs in patients with pulmonary TB against the background of coronavirus infection by analyzing the data of computed tomography (CT) of the chest organs.
MATERIALS AND METHODS. Establishing the diagnosis and treatment of pulmonary TB were carried out in accordance with the Order of the Ministry of Health of Ukraine No. 530. The diagnosis of COVID-19 was established in patients who were treated for pulmonary TB in accordance with the current protocols for the treatment of the coronavirus infection. All patients underwent dynamic CT of the chest organs was performed on the Aquilion TSX-101A scanner (Toshiba, Japan).
RESULTS AND DISCUSSION. Clinical, laboratory and X-ray data of patients with pulmonary TB and COVID-19 were analyzed in dynamics. It has been established that with timely diagnosis and adequate treatment of DSTB of the lungs, viral pneumonia (COVID-19) does not complicate the course of the TB process. The period of the course of COVID-19 is characterized by the continuation of the positive dynamics of the TB process. In patients with TB with multiple drug resistance, viral pneumonia (COVID-19) does not cause serious complications, but the period of the course of COVID-19 is characterized by the absence of positive dynamics of the TB process. With untreated pulmonary TB on the background of viral pneumonia (COVID-19), the disease can become severe and end in death.
CONCLUSIONS. CT of the chest organs in patients with pulmonary TB against the background of COVID-19 is highly informative in monitoring the pathological process, detecting complications and evaluating the effectiveness of treatment.
New and little-known possibilities of edaravone in the treatment of cerebral stroke and extracranial pathology
Authors: Halushko O.A. 
Published: September 2023

BACKGROUND. Edaravone is a free radical scavenger and inhibits lipid peroxidation and thus reduces oxidative damage to brain cells and other organs. Edaravone is mainly known as an effective agent in the treatment of ischemic stroke and amyotrophic lateral sclerosis. OBJECTIVE. To investigate the little-known possibilities of edaravone when it is used in clinical practice. MATERIALS AND METHODS. To solve the task, a search and analysis of full-text articles was conducted in the PubMed, Web of Science, Google Scholar, and Scopus databases. The search was conducted using the key word “edaravone” and included English-language and Ukrainian-language publications over the past 5 years (from April 2018 to April 2023). RESULTS. A total of 518 publications were identified and analyzed. Edaravone has been found to improve the clinical course and may be useful in the treatment of central nervous system diseases, depression, post-traumatic stress disorder, cognitive dysfunction, oncological and infectious diseases (in particular, coronavirus disease), many poisonings, etc. All this dictates the need for further clinical studies to explore new and unexpected possibilities of edaravone.
High-stability solid solution perovskite (1-x) Bi0.2Sr0.5La0.3TiO3- xLaMnO3 (0.05≤ × ≤0.2) for wide-temperature NTC thermistors
Authors: Liu Ting , Yang Guanghua , Ma Juan , Zhang Huimin , Zhang Min , Chang Aimin 
Published: September 2023

The development of negative temperature coefficient (NTC) thermistor materials with a wide range of operating temperatures, high resistance (R), low thermal content (B) and good stability is significant for improving the overall performance of NTC thermistors. Traditional NTC thermistors materials are of the spinel, however, their practical applications are commonly limited to temperatures below approximately 200°C.In this study, it was found that a novel perovskite-structured solid solution (1-x)Bi0.2Sr0.5La0.3TiO3-xLaMnO3 (0.05 ≤ × ≤ 0.2) (BSLT-LM) showed good NTC performance from room temperature to high temperature (600°C) due to the stable structure at high temperatures. The ρ25, ρ100, ρ600 and B25/100, B25/600 constants of Bi0.2Sr0.5La0.3TiO3-0.1LaMnO3 NTC thermistors are approximately 1.76 × 108 Ω cm, 1.13 × 107 Ω cm, 9.89 × 102 Ω cm, 4063.91 K, 5472.34 K, respectively. The electrical conductivity of these solid solution refers to the electronic transition between Mn3+ and Mn4+, and oxygen vacancies. These results demonstrate the tremendous potential of perovskite-structured (1-x) Bi0.3Sr0.5La0.2TiO3-xLaMnO3 thermistor ceramics with NTC performance.
Impact of mutagenesis and lateral gene transfer processes in bacterial susceptibility to phage in food biocontrol and phage therapy
Authors: López-Pérez Júlia , Otero Jennifer , Sánchez-Osuna Miquel , Erill Ivan , Cortés Pilar , Llagostera Montserrat 
Published: September 2023

IntroductionThe emergence of resistance and interference mechanisms to phage infection can hinder the success of bacteriophage-based applications, but the significance of these mechanisms in phage therapy has not been determined. This work studies the emergence of Salmonella isolates with reduced susceptibility to a cocktail of three phages under three scenarios: i) Salmonella cultures (LAB), ii) biocontrol of cooked ham slices as a model of food safety (FOOD), and iii) oral phage therapy in broilers (PT).MethodsS. Typhimurium ATCC 14028 RifR variants with reduced phage susceptibility were isolated from the three scenarios and conventional and molecular microbiology techniques were applied to study them.Results and discussionIn LAB, 92% of Salmonella isolates lost susceptibility to all three phages 24 h after phage infection. This percentage was lower in FOOD, with 4.3% of isolates not susceptible to at least two of the three phages after seven days at 4°C following phage treatment. In PT, 9.7% and 3.3 % of isolates from untreated and treated broilers, respectively, displayed some mechanism of interference with the life cycle of some of the phages. In LAB and FOOD scenarios, resistant variants carrying mutations in rfc and rfaJ genes involved in lipopolysaccharide synthesis (phage receptor) were identified. However, in PT, the significant decrease of EOP, ECOI, and burst size observed in isolates was prompted by lateral gene transfer of large IncI1 plasmids, which may encode phage defense mechanisms. These data indicate that the acquisition of specific conjugative plasmids has a stronger impact than mutagenesis on the emergence of reduced phage-susceptibility bacteria in certain environments. In spite of this, neither mechanism seems to significantly impair the success of Salmonella biocontrol and oral phage therapy.
A rapid and high-throughput multiplex genetic detection assay for detection, semi-quantification and virulence genotyping of Helicobacter pylori in non-invasive oral samples
Authors: Chi Wenjing , Wang Su , Liu Tao , Jiang Wenrong , Ding Li , Miao Yingxin , Yang Feng , Zhang Jinghao , Ji Danian , Xiao Zili , Zhu Haowei , Wu Yong , Bao Zhijun , Zhao Hu , Wang Shiwen 
Published: September 2023

AimThis study established a high-throughput multiplex genetic detection assay (HMGA) for rapid identification, semi-quantification and virulence analysis of Helicobacter pylori directly from the clinical non-invasive oral samples.MethodsThe gastric mucosa and oral samples were collected from 242 patients in Shanghai from 2021 to 2022. All the samples were detected by routine clinical tests for H. pylori and Sanger sequenced for inconsistent results. A new multiplex PCR assay providing results within 4 hours was designed and optimized involving fluorescent dye-labeled specific primers targeted 16S rRNA gene, semi-quantitative gene ureC and 10 virulence genes of H. pylori. Semi-quantification was carried out by simulating the serial 10-fold dilutions of positive oral samples, and the H. pylori loads in different clinical samples were further compared. The mixed plasmids of virulence genes vacA s1, vacA m1 and vacA m2 were used to evaluate the performance on different genotypes. The consistency of 10 virulence genes in gastric mucosa, saliva, mouthwash and dental plaque of H. pylori-positive patients was compared.ResultsThe non-invasive HMGA was highly specific for detection of all 12 targets of H. pylori and human internal reference gene β-globin, and the sensitivity to all target genes could reach 10 copies/μL. Compared with routine clinical tests and sequencing, non-invasive HMGA has a high level (&gt;0.98) of sensitivity, specificity, accuracy, PPV, NPV and kappa coefficient for direct detection of H. pylori in oral samples. Moreover, by detecting peak area levels of ureC, it was confirmed that the H. pylori loads in gastric mucosa were significantly higher than those of the three kinds of oral samples (p&lt;0.05). We also found that 45.0% (91/202) of patients had different H. pylori virulence genes in different oral samples. The concordance of positive detection rates of each virulence gene between saliva and gastric mucosa was more than 78% (p&lt;0.05).ConclusionThe non-invasive HMGA proved to be a reliable method for the rapid H. pylori identification, semi-quantification and detection of 10 virulence genes directly in oral samples, providing a new idea for non-invasive detection of H. pylori.
Traditional Chinese medicine Dingkun pill to increase fertility in women with a thin endometrium—a prospective randomized study
Authors: Jin Fengyu , Ruan Xiangyan , Qin Shuang , Xu Xin , Yang Yu , Gu Muqing , Li Yanqiu , Cheng Jiaojiao , Du Juan , Yin Xiaodan , Mueck Alfred O. 
Published: September 2023

ObjectiveThe aim of this study is to optimize the treatment methods of infertility, which is suggested to be mainly caused by thin endometrium, using a special form of traditional Chinese medicine, the Dingkun pill (DKP), to increase the beneficial endometrial effect of conventional hormone/progestogen therapy.MethodsA total of 307 patients visiting our specialized gynecological endocrinology department because of infertility, which we suggested to be caused by thin endometrium [endometrial thickness (EMT) &lt; 7 mm], were randomly assigned to the experimental group and the control group. The experimental group was treated with estradiol + sequential dydrogesterone + DKP (every day); the control group received hormonal treatment without the Chinese medicine. All patients were monitored in terms of follicle diameter, EMT, and endometrial type every 2 days from the 8th to the 10th day of the menstrual cycle until ovulation day during three menstrual cycles. Serum progesterone levels on 7–8 days after ovulation were measured, and the cumulative pregnancy rate during three menstrual cycles between the two groups was compared.ResultsEMT on ovulation day in the experimental group was significantly higher than that in the control group (7.88 vs. 7.15 mm; p &lt; 0.001). The proportion of type A and type B endometrium in total was significantly higher in the experimental group than that in the control group (83.2% vs. 77.7%; p &lt; 0.05). Progesterone levels were significantly higher in the experimental group than those in the control group (10.874 vs. 10.074 ng/mL; p &lt; 0.001). The cumulative pregnancy rate, the main outcome of the study, was significantly higher in the experimental group than that in the control group (29.2% vs. 15.7%; p &lt; 0.05).ConclusionDKP added to conventional estrogen/progestogen therapy can significantly improve EMT and luteal function in patients attending due to infertility. Because this regimen increased the cumulative pregnancy rate in our study, we conclude that DKP can be used to increase the so-called “thin endometrium infertility”.
Physical exercise as treatment for adults with type 2 diabetes: a rapid review
Authors: Ribeiro Andressa Karoline Pinto de Lima , Carvalho Josilayne Patrícia Ramos , Bento-Torres Natáli Valim Oliver 
Published: September 2023

BackgroundType 2 diabetes mellitus (T2DM) is a leading cause of disability-adjusted life years (DALY). Physical exercise is an effective non-pharmacological intervention to promote glycaemic control in T2DM. However, the optimal exercise parameters for glycemic control in individuals with T2DM remain unclear.ObjectiveThis study aimed to analyze the relationship between physical training variables – frequency, intensity, type, duration, volume, and progression – and glycemic control in individuals with T2DM.MethodsA rapid systematic literature review was conducted on PubMed and LILACS databases. The PICOT strategy was employed to define the inclusion criteria. Eligible studies had to assess the impact of exercise parameters (frequency, intensity, type, duration, volume, and progression) on glycemic control indicators, primarily glycosylated hemoglobin (HbA1c). Randomized and non-randomized clinical trials were included in the review. The methodological quality of each study was assessed using the PEDro scale (PROSPERO - CRD 42021262614).ResultsOut of 1188 papers initially identified, 18 reports met the inclusion criteria and were included in the analysis. A total of 1,228 participants with T2DM (1086 in exercise groups) were included in the selected studies. Among these studies, 16 (88.9%) were RCTs and 2 (11.1%) were nRCTs. The age of participants ranged from 43.1 and 68.9 years, and the average intervention duration was 16.8 weeks. Data on adherence to the intervention, adverse events, detailed intervention protocol, and its impacts on glycaemic control, lipid profile, blood pressure, anthropometric measures, medication, body composition, and physical fitness are reported.ConclusionThe evidence supports the safety and effectiveness of physical exercises as non-pharmacological interventions for glycemic control. Aerobic, resistance and combined training interventions were associated with reductions in HbA1c and fasting glucose. The diversity of the physical exercise intervention protocols investigated in the studies included in this review is an important limitation to generalizing evidence-based practice. The call for action is mandatory to implement large-scale education programs on the prevention of diabetes and public health policies aimed to include well-planned and supervised exercise programs as an essential part of the primary prevention of type 2 diabetes.Systematic Review RegistrationPROSPERO, identifier (CRD42021262614).
Insulin resistance and central obesity determine hepatic steatosis and explain cardiovascular risk in steatotic liver disease
Authors: Semmler Georg , Balcar Lorenz , Wernly Sarah , Völkerer Andreas , Semmler Lorenz , Hauptmann Laurenz , Wernly Bernhard , Aigner Elmar , Niederseer David , Datz Christian 
Published: September 2023

BackgroundMetabolic dysfunction-associated steatotic liver disease (MASLD) has recently been proposed to replace non-alcoholic fatty liver disease and focus on patients with progressive disease due to the presence of metabolic dysfunction. However, it is unclear whether the new definition actually identifies patients with hepatic steatosis at increased cardiovascular risk.MethodsA total of 4,286 asymptomatic subjects from the SAKKOPI study aged 45–80 years undergoing screening colonoscopy were analyzed. Steatosis was diagnosed by abdominal ultrasound. MASLD was diagnosed according to the recent expert consensus. Insulin resistance was assessed by homeostasis model assessment-insulin resistance score (HOMA-IR) (cutoff: ≥2.5), subclinical inflammation was estimated by ferritin/CRP/uric acid, and cardiovascular risk was assessed using SCORE2/ASCVD.ResultsMean age was 59.4 ± 8.5 years, 51.6% were male; mean BMI was 27.0 ± 4.5 kg/m², 9.2% had type 2 diabetes mellitus. In total, 1,903 (44.4%) were diagnosed with hepatic steatosis and were characterized by more severe metabolic dysfunction including insulin resistance (47.1% vs. 12.2%, p &lt; 0.001) and central obesity (waist circumference ≥102/88 cm, 71.8% vs. 37.1%, p &lt; 0.001). This translated into higher (subclinical) inflammation (ferritin 153 vs. 95 mg/dL, p &lt; 0.001, uric acid 6.3 mg/dL vs. 5.2 mg/dL, p &lt; 0.001) and 10-year cardiovascular risk (SCORE2 7.8 points vs. 5.1 points, p &lt; 0.001, ASCVD 17.9 points vs. 10.8 points, p &lt; 0.001). 99.0% of subjects with steatosis met the MASLD definition, 95.4% met the MAFLD definition, and 53.6% met the definition of metabolic syndrome, while 95.4% of subjects without steatosis also met the MASLD criteria for metabolic dysfunction compared to 69.0% and 17.4% who met the MAFLD and metabolic syndrome criteria, respectively. Forward stepwise regression indicated that waist circumference, HOMA-IR, and triglycerides were most relevant in explaining the presence of hepatic steatosis across all subgroups of increasing metabolic dysfunction. At the same time, hepatic steatosis was not associated with cardiovascular risk in the overall cohort (SCORE2: B = 0.060, 95% CI: −0.193–0.314, and p = 0.642) and in patients with metabolic dysfunction after adjusting for age, sex, and these three metabolic dysfunction components.ConclusionAlthough hepatic steatosis is associated with increased central obesity and insulin resistance, metabolic dysfunction per se rather than hepatic steatosis explains cardiovascular risk in these patients.
Cytokines impact natural killer cell phenotype and functionality against glioblastoma in vitro
Authors: Sivonen Minna , Sirviö Katja A. , Wojciechowski Sara , Kailaanmäki Anssi , Kaipainen Satu , Bailey Aubrey , Villalba Martin , Kekarainen Tuija 
Published: September 2023

ObjectiveNatural killer (NK) cells are a part of the innate immune system and first-line defense against cancer. Since they possess natural mechanisms to recognize and kill tumor cells, NK cells are considered as a potential option for an off-the-shelf allogeneic cell-based immunotherapy. Here, our objective was to identify the optimal cytokine-based, feeder-free, activation and expansion protocol for cytotoxic NK cells against glioblastoma in vitro.MethodsNK cells were enriched from human peripheral blood and expanded for 16 days with different activation and cytokine combinations. The expansion conditions were evaluated based on NK cell viability, functionality, expansion rate and purity. The cytotoxicity and degranulation of the expanded NK cells were measured in vitro from co‑cultures with the glioma cell lines U‑87 MG, U‑87 MG EGFR vIII, LN-229, U-118 and DK-MG. The best expansion protocols were selected from ultimately 39 different conditions: three magnetic cell‑selection steps (Depletion of CD3+ cells, enrichment of CD56+ cells, and depletion of CD3+ cells followed by enrichment of CD56+ cells); four activation protocols (continuous, pre-activation, re-activation, and boost); and four cytokine combinations (IL-2/15, IL‑21/15, IL‑27/18/15 and IL-12/18/15).ResultsThe expansion rates varied between 2-50-fold, depending on the donor and the expansion conditions. The best expansion rate and purity were gained with sequential selection (Depletion of CD3+ cells and enrichment of CD56+ cells) from the starting material and pre-activation with IL‑12/18/15 cytokines, which are known to produce cytokine-induced memory-like NK cells. The cytotoxicity of these memory-like NK cells was enhanced with re-activation, diminishing the donor variation. The most cytotoxic NK cells were produced when cells were boosted at the end of the expansion with IL-12/18/15 or IL-21/15.ConclusionAccording to our findings the ex vivo proliferation capacity and functionality of NK cells is affected by multiple factors, such as the donor, composition of starting material, cytokine combination and the activation protocol. The cytokines modified the NK cells' phenotype and functionality, which was evident in their reactivity against the glioma cell lines. To our knowledge, this is the first comprehensive comparative study performed to this extent, and these findings could be used for upscaling clinical NK cell manufacturing.