bluuebunny/crossref_metadata_2025_split huggingface dataset:
Single cell analysis in head and neck cancer reveals potential immune evasion mechanisms during early metastasis
Authors: Quah Hong Sheng , Cao Elaine Yiqun , Suteja Lisda , Li Constance H. , Leong Hui Sun , Chong Fui Teen , Gupta Shilpi , Arcinas Camille , Ouyang John F. , Ang Vivian , Celhar Teja , Zhao Yunqian , Tay Hui Chen , Chan Jerry , Takahashi Takeshi , Tan Daniel S. W. , Biswas Subhra K. , Rackham Owen J. L. , Iyer N. Gopalakrishna 
Published: March 2023

AbstractProfiling tumors at single-cell resolution provides an opportunity to understand complexities underpinning lymph-node metastases in head and neck squamous-cell carcinoma. Single-cell RNAseq (scRNAseq) analysis of cancer-cell trajectories identifies a subpopulation of pre-metastatic cells, driven by actionable pathways including AXL and AURK. Blocking these two proteins blunts tumor invasion in patient-derived cultures. Furthermore, scRNAseq analyses of tumor-infiltrating CD8 + T-lymphocytes show two distinct trajectories to T-cell dysfunction, corroborated by their clonal architecture based on single-cell T-cell receptor sequencing. By determining key modulators of these trajectories, followed by validation using external datasets and functional experiments, we uncover a role for SOX4 in mediating T-cell exhaustion. Finally, interactome analyses between pre-metastatic tumor cells and CD8 + T-lymphocytes uncover a putative role for the Midkine pathway in immune-modulation and this is confirmed by scRNAseq of tumors from humanized mice. Aside from specific findings, this study demonstrates the importance of tumor heterogeneity analyses in identifying key vulnerabilities during early metastasis.
Evanescent field trapping and propulsion of Janus particles along optical nanofibers
Authors: Tkachenko Georgiy , Truong Viet Giang , Esporlas Cindy Liza , Sanskriti Isha , Nic Chormaic Síle 
Published: March 2023

AbstractSmall composite objects, known as Janus particles, drive sustained scientific interest primarily targeted at biomedical applications, where such objects act as micro- or nanoscale actuators, carriers, or imaging agents. A major practical challenge is to develop effective methods for the manipulation of Janus particles. The available long-range methods mostly rely on chemical reactions or thermal gradients, therefore having limited precision and strong dependency on the content and properties of the carrier fluid. To tackle these limitations, we propose the manipulation of Janus particles (here, silica microspheres half-coated with gold) by optical forces in the evanescent field of an optical nanofiber. We find that Janus particles exhibit strong transverse localization on the nanofiber and much faster propulsion compared to all-dielectric particles of the same size. These results establish the effectiveness of near-field geometries for optical manipulation of composite particles, where new waveguide-based or plasmonic solutions could be envisaged.
Flexible and transparent thin-film light-scattering photovoltaics about fabrication and optimization for bifacial operation
Authors: Choi Soo-Won , Park Jae-Ho , Seo Ji-Woo , Mun Chaewon , Kim Yonghun , Song Pungkeun , Shin Myunghun , Kwon Jung-Dae 
Published: March 2023

AbstractFlexible and transparent thin-film silicon solar cells were fabricated and optimized for building-integrated photovoltaics and bifacial operation. A laser lift-off method was developed to avoid thermal damage during the transfer of light-scattering structures onto colorless polyimide substrates and thus enhance front-incidence photocurrent, while a dual n-type rear window layer was introduced to reduce optical losses, facilitate electron transport for rear incidence, and thus enhance performance during bifacial operation. The introduction of the window layer increased the rear-to-front power conversion efficiency ratio to ~86%. The optimized bifacial power conversion efficiency for front and rear irradiances of 1 and 0.3 sun, respectively, equaled 6.15%, and the average transmittance within 500–800 nm equaled 36.9%. Additionally, the flexible and transparent solar cells fabricated using laser lift-off exhibited good mechanical reliability (i.e., sustained 500 cycles at a bending radius of 6 mm) and were therefore suitable for building-integrated photovoltaics.
Extracellular vesicles released from ganglioside GD2-expressing melanoma cells enhance the malignant properties of GD2-negative melanomas
Authors: Yesmin Farhana , Furukawa Keiko , Kambe Mariko , Ohmi Yuhsuke , Bhuiyan Robiul Hasan , Hasnat Mohammad Abul , Mizutani Momoka , Tajima Orie , Hashimoto Noboru , Tsuchida Akiko , Kaneko Kei , Furukawa Koichi 
Published: March 2023

AbstractExosomes (small extracellular vesicles: EVs) have attracted increasing attention from basic scientists and clinicians since they play important roles in cell-to-cell communication in various biological processes. Various features of EVs have been elucidated regarding their contents, generation and secretion mechanisms, and functions in inflammation, regeneration, and cancers. These vesicles are reported to contain proteins, RNAs, microRNAs, DNAs, and lipids. Although the roles of individual components have been rigorously studied, the presence and roles of glycans in EVs have rarely been reported. In particular, glycosphingolipids in EVs have not been investigated to date. In this study, the expression and function of a representative cancer-associated ganglioside, GD2, in malignant melanomas was investigated. Generally, cancer-associated gangliosides have been shown to enhance malignant properties and signals in cancers. Notably, EVs derived from GD2-expressing melanomas enhanced the malignant phenotypes of GD2-negative melanomas, such as cell growth, invasion, and cell adhesion, in a dose-dependent manner. The EVs also induced increased phosphorylation of signaling molecules such as EGF receptor and focal adhesion kinase. These results suggest that EVs released from cancer-associated ganglioside-expressing cells exert many functions that have been reported as a function of these gangliosides and regulate microenvironments, including total aggravation of heterogeneous cancer tissues, leading to more malignant and advanced cancer types.
Investigating TSPO levels in occupation-related posttraumatic stress disorder
Authors: Watling Sarah E. , Gill Talwinder , Gaudette Erin V. , Richardson J. Don , McCluskey Tina , Tong Junchao , Meyer Jeffrey H. , Warsh Jerry , Jetly Rakesh , Hutchison Michael G. , Rhind Shawn G. , Houle Sylvain , Kish Stephen J. , Boileau Isabelle 
Published: March 2023

AbstractMicroglia are immune brain cells implicated in stress-related mental illnesses including posttraumatic stress disorder (PTSD). Their role in the pathophysiology of PTSD, and on neurobiological systems that regulate stress, is not completely understood. We tested the hypothesis that microglia activation, in fronto-limbic brain regions involved in PTSD, would be elevated in participants with occupation-related PTSD. We also explored the relationship between cortisol and microglia activation. Twenty participants with PTSD and 23 healthy controls (HC) completed positron emission tomography (PET) scanning of the 18-kDa translocator protein (TSPO), a putative biomarker of microglia activation using the probe [18F]FEPPA, and blood samples for measurement of cortisol. [18F]FEPPA VT was non-significantly elevated (6.5–30%) in fronto-limbic regions in PTSD participants. [18F]FEPPA VT was significantly higher in PTSD participants reporting frequent cannabis use compared to PTSD non-users (44%, p = 0.047). Male participants with PTSD (21%, p = 0.094) and a history of early childhood trauma (33%, p = 0.116) had non-significantly higher [18F]FEPPA VT. Average fronto-limbic [18F]FEPPA VT was positively related to cortisol (r = 0.530, p = 0.028) in the PTSD group only. Although we did not find a significant abnormality in TSPO binding in PTSD, findings suggest microglial activation might have occurred in a subgroup who reported frequent cannabis use. The relationship between cortisol and TSPO binding suggests a potential link between hypothalamic–pituitary–adrenal-axis dysregulation and central immune response to trauma which warrants further study.
Towards passive non-line-of-sight acoustic localization around corners using uncontrolled random noise sources
Authors: Boger-Lombard Jeremy , Slobodkin Yevgeny , Katz Ori 
Published: March 2023

AbstractNon-line-of-sight (NLoS) imaging is an important challenge in many fields ranging from autonomous vehicles and smart cities to defense applications. Several recent works in optics and acoustics tackle the challenge of imaging targets hidden from view (e.g. placed around a corner) by measuring time-of-flight information using active SONAR/LiDAR techniques, effectively mapping the Green functions (impulse responses) from several controlled sources to an array of detectors. Here, leveraging passive correlations-based imaging techniques (also termed ’acoustic daylight imaging’), we study the possibility of acoustic NLoS target localization around a corner without the use of controlled active sources. We demonstrate localization and tracking of a human subject hidden around a corner in a reverberating room using Green functions retrieved from correlations of broadband uncontrolled noise sources recorded by multiple detectors. Our results demonstrate that for NLoS localization controlled active sources can be replaced by passive detectors as long as a sufficiently broadband noise is present in the scene.
Repertoire of P-glycoprotein drug transporters in the zoonotic nematode Toxocara canis
Authors: Jesudoss Chelladurai Jeba R. J. , Martin Katy A. , Vardaxis Pam , Reinemeyer Craig , Vijayapalani Paramasivan , Robertson Alan P. , Brewer Matthew T. 
Published: March 2023

AbstractToxocara canis has a complex lifecycle including larval stages in the somatic tissue of dogs that tolerate macrocyclic lactones. In this study, we investigated T. canis permeability glycoproteins (P-gps, ABCB1) with a putative role in drug tolerance. Motility experiments demonstrated that while ivermectin failed to abrogate larval movement, the combination of ivermectin and the P-gp inhibitor verapamil induced larval paralysis. Whole organism assays revealed functional P-gp activity in larvae which were capable of effluxing the P-gp substrate Hoechst 33342 (H33342). Further investigation of H33342 efflux demonstrated a unique rank order of potency for known mammalian P-gp inhibitors, suggesting that one or more of the T. canis transporters has nematode-specific pharmacological properties. Analysis of the T. canis draft genome resulted in the identification of 13 annotated P-gp genes, enabling revision of predicted gene names and identification of putative paralogs. Quantitative PCR was used to measure P-gp mRNA expression in adult worms, hatched larvae, and somatic larvae. At least 10 of the predicted genes were expressed in adults and hatched larvae, and at least 8 were expressed in somatic larvae. However, treatment of larvae with macrocyclic lactones failed to significantly increase P-gp expression as measured by qPCR. Further studies are needed to understand the role of individual P-gps with possible contributions to macrocyclic lactone tolerance in T. canis.
Protection of pipeline below pavement subjected to traffic induced dynamic response
Authors: Chaudhuri Chaidul Haque , Choudhury Deepankar 
Published: March 2023

AbstractFailure of pipelines below road pavement results to the disruption of both the traffic movement and the consumers of the pipelines. Intermediate safeguard layer can be used to protect the pipeline from heavy traffic loads. The present study proposed analytical solutions to obtain the dynamic response of buried pipe below road pavement with and without considering safeguard based on the concept of triple and double beam system respectively. Pavement layer, safeguard and the pipeline are considered as Euler Bernoulli’s beam. Advanced soil model is used (viscoelastic foundation with shear interaction between springs) to model the surrounding soil. Self-weight of soil is also considered in the present study. The obtained governing coupled differential equations are solved adopting finite sine Fourier transform, Laplace transform and their inverse transformation. The proposed formulation is initially verified with the past numerical and analytical studies and then validated with the three-dimensional finite element based numerical analysis. From parametric study it is perceived that the stability of the pipe can be significantly increased by providing intermediate barrier. Further, pipe deformation is increases with increasing traffic loads. At very high-speed range (> 60 m s−1), pipe deformation is significantly rises with increasing traffic speed. The present study can be useful in preliminary design stage before performing rigorous and expensive numerical or experimental study.
Lack of furosemide responsiveness predict severe acute kidney injury after liver transplantation
Authors: Cai Li , Shu Liu , Yujun Zhao , Ke Cheng , Qiang Wang 
Published: March 2023

AbstractAcute kidney injury (AKI) remains to be a common but severe complication after liver transplantation (LT). However, there are still few clinically validated biomarkers. A total of 214 patients who underwent routine furosemide (1–2 mg/kg) after LT were retrospectively included. The urine output during the first 6 h was recorded to evaluate the predictive value of AKI stage 3 and renal replacement therapy (RRT). 105 (49.07%) patients developed AKI, including 21 (9.81%) progression to AKI stage 3 and 10 (4.67%) requiring RRT. The urine output decreased with the increasing severity of AKI. The urine output of AKI stage 3 did not significantly increase after the use of furosemide. The area under the receiver operator characteristic (ROC) curves for the total urine output in the first hour to predict progression to AKI stage 3 was 0.94 (p < 0.001). The ideal cutoff for predicting AKI progression during the first hour was a urine volume of less than 200 ml with a sensitivity of 90.48% and specificity of 86.53%. The area under the ROC curves for the total urine output in the six hours to predict progression to RRT was 0.944 (p < 0.001). The ideal cutoff was a urine volume of less than 500 ml with a sensitivity of 90% and specificity of 90.91%. Severe AKI after liver transplantation seriously affects the outcome of patients. Lack of furosemide responsiveness quickly and accurately predict AKI stage 3, and patients requiring RRT after the operation.
Effect of ocean acidification on the growth, response and hydrocarbon degradation of coccolithophore-bacterial communities exposed to crude oil
Authors: Fahmi Afiq Mohd , Summers Stephen , Jones Martin , Bowler Bernard , Hennige Sebastian , Gutierrez Tony 
Published: March 2023

AbstractHydrocarbon-degrading bacteria, which can be found living with eukaryotic phytoplankton, play a pivotal role in the fate of oil spillage to the marine environment. Considering the susceptibility of calcium carbonate-bearing phytoplankton under future ocean acidification conditions and their oil-degrading communities to oil exposure under such conditions, we investigated the response of non-axenic E. huxleyi to crude oil under ambient versus elevated CO2 concentrations. Under elevated CO2 conditions, exposure to crude oil resulted in the immediate decline of E. huxleyi, with concomitant shifts in the relative abundance of Alphaproteobacteria and Gammaproteobacteria. Survival of E. huxleyi under ambient conditions following oil enrichment was likely facilitated by enrichment of oil-degraders Methylobacterium and Sphingomonas, while the increase in relative abundance of Marinobacter and unclassified Gammaproteobacteria may have increased competitive pressure with E. huxleyi for micronutrient acquisition. Biodegradation of the oil was not affected by elevated CO2 despite a shift in relative abundance of known and putative hydrocarbon degraders. While ocean acidification does not appear to affect microbial degradation of crude oil, elevated mortality responses of E. huxleyi and shifts in the bacterial community illustrates the complexity of microalgal-bacterial interactions and highlights the need to factor these into future ecosystem recovery projections.
Assessing the structure of the posterior visual pathway in bilateral macular degeneration
Authors: Brown Holly D. H. , Gale Richard P. , Gouws André D. , Vernon Richard J. W. , Airody Archana , Hanson Rachel L. W. , Baseler Heidi A. , Morland Antony B. 
Published: March 2023

AbstractMacular degeneration (MD) embodies a collection of disorders causing a progressive loss of central vision. Cross-sectional MRI studies have revealed structural changes in the grey and white matter in the posterior visual pathway in MD but there remains a need to understand how such changes progress over time. To that end we assessed the posterior pathway, characterising the visual cortex and optic radiations over a ~ 2-year period in MD patients and controls. We performed cross-sectional and longitudinal analysis of the former. Reduced cortical thickness and white matter integrity were observed in patients compared to controls, replicating previous findings. While faster, neither the rate of thinning in visual cortex nor the reduction in white matter integrity during the ~ 2-year period reached significance. We also measured cortical myelin density; cross-sectional data showed this was higher in patients than controls, likely as a result of greater thinning of non-myelinated tissue in patients. However, we also found evidence of a greater rate of loss of myelin density in the occipital pole in the patient group indicating that the posterior visual pathway is at risk in established MD. Taken together, our results revealed a broad decline in grey and white matter in the posterior visual pathway in bilateral MD; cortical thickness and fractional anisotropy show hints of an accelerated rate of loss also, with larger effects emerging in the occipital pole.
The long-term reproducibility of the white-coat effect on blood pressure as a continuous variable from the Ohasama Study
Authors: Satoh Michihiro , Yoshida Tomoya , Metoki Hirohito , Murakami Takahisa , Tatsumi Yukako , Hirose Takuo , Takabatake Kyosuke , Tsubota-Utsugi Megumi , Hara Azusa , Nomura Kyoko , Asayama Kei , Kikuya Masahiro , Hozawa Atsushi , Imai Yutaka , Ohkubo Takayoshi 
Published: March 2023

AbstractThere is little information about the reproducibility of the white coat effect, which was treated as a continuous variable. To investigate a long-term interval reproducibility of the white-coat effect as a continuous variable. We selected 153 participants without antihypertensive treatment (men, 22.9%; age, 64.4 years) from the general population of Ohasama, Japan, to assess the repeatedly measured white-coat effect (the difference between blood pressures at the office and home) in a 4-year interval. The reproducibility was assessed by testing the intraclass correlation coefficient (two-way random effect model-single measures). The white-coat effect for systolic/diastolic blood pressure slightly decreased by 0.17/1.56 mmHg at the 4-year visit on average. The Bland–Altman plots showed no significant systemic error for the white-coat effects (P ≥ 0.24). The intraclass correlation coefficient (95% confidence interval) of the white-coat effect for systolic blood pressure, office systolic blood pressure, and home systolic blood pressure were 0.41 (0.27–0.53), 0.64 (0.52–0.74), and 0.74 (0.47–0.86), respectively. Change in the white-coat effect was mainly affected by a change in office blood pressure. Long-term reproducibility of the white-coat effect is limited in the general population without antihypertensive treatment. The change in the white-coat effect is mainly caused by office blood pressure variation.
Metabolically healthy and unhealthy obesity and the development of lung dysfunction
Authors: Song Jae-Uk , Lee Jonghoo , Lim Si-Young , Gil Hyun-Il , Chang Yoosoo , Ryu Seungho 
Published: March 2023

AbstractWe investigated the association of metabolically healthy (MH) and unhealthy (MU) obesity with incident lung dysfunction. This cohort study included 253,698 Korean lung disease-free adults (mean age, 37.4 years) at baseline. Spirometry-defined lung dysfunction was classified as a restrictive pattern (RP) or obstructive pattern (OP). We defined obesity as BMI ≥ 25 kg/m2 and MH as the absence of any metabolic syndrome components with a homeostasis model assessment of insulin resistance < 2.5: otherwise, participants were considered MU. During a median follow-up of 4.9 years, 10,775 RP cases and 7140 OP cases develped. Both MH and MU obesity showed a positive association with incident RP, with a stronger association in the MU than in the MH group (Pinteraction = 0.001). Multivariable-adjusted hazard ratios (95% CI) for incident RP comparing obesity to the normal-weight category was 1.15 (1.05–1.25) among the MH group and 1.38 (1.30–1.47) among MU group. Conversely, obesity was inversely associated with OP because of a greater decline in forced vital capacity than forced expiratory volume in 1 s. Both MH and MU obesity were positively associated with RP. However, the associations between obesity, metabolic health, and lung functions might vary depending on the type of lung disease.
Time course of muscle activation, energetics and mechanics of running in minimalist and traditional cushioned shoes during level running
Authors: Udin Gilles , Fernandez Menendez Aitor , Hoyois Jonas , Chevalier Mathias , Malatesta Davide 
Published: March 2023

AbstractThe study aimed to compare the ankle muscles activation, biomechanics and energetics of running in male runners during submaximal level run using minimalist (MinRS) and traditional cushioned (TrdRS) running shoes. During 45-min running in MinRS and TrdRS, the ankle muscles pre- and co-activation, biomechanics, and energetics of running of 16 male endurance runners (25.5 ± 3.5 yr) were assessed using surface electromyography (tibialis anterior and gastrocnemius lateralis), instrumented treadmill and indirect calorimetry, respectively. The net energy cost of running (Cr) was similar for both conditions (P = 0.25) with a significant increase over time (P < 0.0001). Step frequency (P < 0.001), and total mechanical work (P = 0.001) were significantly higher in MinRS than in TrdRS with no evolution over time (P = 0.28 and P = 0.85, respectively). The ankle muscles pre- and co-activation during the contact phase did not differ between the two shoe conditions (P ≥ 0.33) or over time (P ≥ 0.15). In conclusion, during 45-min running, Cr and muscle pre- and co-activation were not significantly different between MinRS and TrdRS with significantly higher step frequency and total mechanical work noted in the former than in the latter. Moreover, Cr significantly increased during the 45-min trial in both shoe conditions along with no significant change over time in muscle activation and biomechanical variables.