bluuebunny/crossref_metadata_2025_split huggingface dataset:
Risk of Venous Thromboembolism With Gout Flares
Authors: Cipolletta Edoardo , Tata Laila J. , Nakafero Georgina , Avery Anthony J. , Mamas Mamas A. , Abhishek Abhishek 
Published: February 2023

ObjectivePrevious studies demonstrated that the risk of venous thromboembolism (VTE) is increased in patients with gout, but not whether there was a temporal association between gout flare and VTE. This study was undertaken to evaluate potential temporal associations between gout flare and VTE.MethodsData were obtained from electronic primary‐care records from the UK's Clinical Practice Research Datalink, which links data from hospitalization and mortality registers. Using self‐controlled case series analysis adjusted for season and age, we evaluated the temporal association between gout flare and VTE. The 90 days after primary‐care consultation or hospitalization for gout flare was designated the exposed period. This was divided into three 30‐day intervals. The baseline period was up to 2 years before the start of and up to 2 years after the end of the exposed period. The association between gout flare and VTE was measured using adjusted incidence rate ratios (IRRs) with 95% confidence intervals (95% CIs).ResultsIn total, 314 patients met the inclusion criteria (age ≥18 years, incident gout, no presence of VTE or use of a primary‐care anticoagulant prescription before the start of the pre‐exposure period). Among the 314 patients, VTE incidence was significantly higher in the exposed period than in the baseline period (adjusted IRR 1.83, 95% CI 1.30–2.59). The adjusted IRR of VTE during the first 30 days after gout flare was 2.31 (95% CI 1.39–3.82) relative to the baseline period. No increase in the adjusted IRRs was observed in days 31–60 (adjusted IRR 1.49, 95% CI 0.79–2.81) and days 61–90 (adjusted IRR 1.67, 95% CI 0.91–3.06) relative to baseline. Results were consistent across sensitivity analyses.ConclusionAmong patients with gout, there was a transient increase in the rate of VTE within 30 days after primary‐care consultation or hospitalization for gout flare.
Efficacy of gemcitabine in combination with nanoparticle albumin‐bound paclitaxel in the treatment of recurrent ovarian cancer: A retrospective single institution review
Authors: Kase Adam M. , Azzouqa Abdel‐Ghani , Kochuveettil Swapna , Colon‐Otero Gerardo 
Published: February 2023

AbstractBackgroundThe objective of this study was to evaluate the efficacy of gemcitabine plus nab‐paclitaxel in patients with recurrent ovarian cancer.MethodsWe performed a single institution retrospective review of patients with recurrent ovarian cancer who were treated with gemcitabine plus nab‐paclitaxel from 2012 to 2018 at the Mayo Clinic in Florida.ResultsTwenty patients were identified and the median PFS for patients treated with gemcitabine plus nab‐paclitaxel was 9 months (95% CI, 5.7–20.7). Overall, 17 of the 20 patients (85%) achieved a clinical benefit (complete response 5%, partial response 55%, or stable disease at 3 months 25%). For platinum‐sensitive disease and platinum‐resistant disease, the median OS were 38.7 months (95% CI, 5.8–63.1) and 31.2 months (95% CI, 12.8–51.8), respectively (p = 0.4306).ConclusionThis well‐tolerated regimen shows promising activity in recurrent ovarian cancer and is a viable option for patients who are intolerant to paclitaxel or carboplatin because of allergic reactions.
Effect of rice leaf stage on tolerance to benzobicyclon in a drill‐seeded production system
Authors: Patterson Jake A. , Norsworthy Jason K. , Butts Thomas R. , Gbur Edward E. 
Published: February 2023

AbstractBenzobicyclon is a new postflood‐applied herbicide that was registered for use in U.S. rice (Oryza sativa L.) in 2021 by Gowan Company. Therefore, research was conducted to evaluate tolerance of commonly grown rice cultivars to benzobicyclon. In 2018 and 2019, field experiments were conducted near Stuttgart, AR to evaluate the influence of growth stage on rice tolerance to benzobicyclon, and to evaluate pure line and hybrid rice tolerance to benzobicyclon following repeated use of acetolactate synthase‐inhibiting herbicides. In one of two years, rice growth stage (leaf number) at application impacted tolerance to benzobicyclon. In 2018, the two‐leaf application of benzobicyclon was generally more injurious to the rice cultivars, ‘CL153’, ‘Diamond’, ‘PVL01’, and ‘CLXL745’ than when applied at four‐leaf or tillering growth stages. In the second year, all rice cultivars, except ‘Rondo’, were not injured by benzobicyclon applied at any growth stage. In both years, benzobicyclon applied to indica rice cultivar Rondo elicited ≥97% crop injury, regardless of application timing. Findings from these experiments suggest that rice cultivar tolerance can vary across environments and that smaller‐sized rice could be more prone to injury than when applications occur at a typical timing for a postflood‐applied herbicide. In general, four‐leaf and tillering rice will exhibit sufficient tolerance to benzobicyclon. It is especially not recommended to apply benzobicyclon on rice cultivars that have a predominant indica‐type genealogical background. Benzobicyclon following repeated applications of acetolactate synthase‐inhibiting herbicides to the pure line cultivar CL153 and the hybrid cultivar CLXL745 did not pose increased risk for injury to rice.
Revealing the Crystal Phase‐Activity Relationship on NiRu Alloy Nanoparticles Encapsulated in N‐Doped Carbon towards Efficient Hydrogen Evolution Reaction
Authors: Yuan Ying , Zheng Lufan , Rong Junfeng , Zhao XiKang , Wu Genghuang , Zhuang Zhongbin 
Published: February 2023

AbstractAdjusting the crystal phase of a metal alloy is an important method to optimize catalytic performance. However, detailed understanding about the phase‐property relationship for the hydrogen evolution reaction (HER) is still limited. In this work, the crystal phase‐activity relationship of NiRu nanoparticles is studied employing N‐doped carbon shell coated NiRu nanoparticles with different phase contents. It is found that the NiRu@NC (mix) with both face‐centred cubic (fcc) and thermodynamically unstable hexagonal close‐packed (hcp) phase NiRu give the best HER performance. Further activity studies demonstrate that hcp NiRu has better HER performance, and NiRu@NC (mix) with rich (∼70 %) hcp phase presented outstanding performance with an overpotential of only 27 mV @ 10 mA ⋅ cm−2. The high HER activity of NiRu@NC (mix) is attributed to the formation of hcp phase. This finding indicates that the regulation of crystal structure can provide a new strategy for optimizing HER activity.
BODIPY‐Perylene Charge Transfer Compounds; Sensitizers for Triplet‐Triplet Annihilation Up‐conversion
Authors: Arellano‐Reyes Ruben Arturo , Prabhakaran Amrutha , Sia Rengel Cane E. , Guthmuller Julien , Jha Keshav Kumar , Yang Tingxiang , Dietzek‐Ivanšić Benjamin , McKee Vickie , Keyes Tia E. 
Published: February 2023

AbstractBODIPY heterochromophores, asymmetrically substituted with perylene and/or iodine at the 2 and 6 positions were prepared and investigated as sensitizers for triplet‐triplet annihilation up conversion (TTA‐UC). Single‐crystal X‐ray crystallographic analyses show that the torsion angle between BODIPY and perylene units lie between 73.54 and 74.51, though they are not orthogonal. Both compounds show intense, charge transfer absorption and emission profiles, confirmed by resonance Raman spectroscopy and consistent with DFT calculations. The emission quantum yield was solvent dependent but the emission profile remained characteristic of CT transition across all solvents explored. Both BODIPY derivatives were found to be effective sensitizers of TTA‐UC with perylene annihilator in dioxane and DMSO. Intense anti‐Stokes emission was observed, and visible by eye from these solvents. Conversely, no TTA‐UC was observed from the other solvents explored, including from non‐polar solvents such as toluene and hexane that yielded brightest fluorescence from the BODIPY derivatives. In dioxane, the power density plots obtained were strongly consistent with TTA‐UC and the power density threshold, the Ith value (the photon flux at which 50 % of ΦTTAUC is achieved), for B2PI was observed to be 2.5x lower than of B2P under optimal conditions, an effect ascribed to the combined influence of spin‐orbit charge transfer intersystem crossing (SOCT‐ISC) and heavy metal on the triplet state formation for B2PI.
NHC‐Stabilised Parent Tripentelyltrielanes
Authors: Szlosek Robert , Weinhart Michael A. K. , Balázs Gábor , Seidl Michael , Zimmermann Lisa , Scheer Manfred 
Published: February 2023

AbstractA missing family of the extremely air sensitive tripentelyltrielanes was discovered. Their stabilisation was achieved by using the bulky NHC IDipp (NHC=N‐heterocyclic carbene, IDipp=1,3‐bis(2,6‐diisopropylphenyl)‐imidazolin‐2‐ylidene). The tripentelylgallanes and tripentelylalanes IDipp ⋅ Ga(PH2)3 (1 a), IDipp ⋅ Ga(AsH2)3 (1 b), IDipp ⋅ Al(PH2)3 (2 a) and IDipp ⋅ Al(AsH2)3 (2 b) were synthesised by salt metathesis of IDipp ⋅ ECl3 (E=Al, Ga, In) with alkali metal pnictogenides such as NaPH2/LiPH2 ⋅ DME and KAsH2, respectively. Moreover, the detection of the first NHC‐stabilised tripentelylindiumane IDipp ⋅ In(PH2)3 (3) was possible by multinuclear NMR spectroscopy. Initial investigations of the coordination ability of these compounds resulted in the successful isolation of the coordination compound [IDipp ⋅ Ga(PH2)2(μ3‐PH2{HgC6F4}3)] (4) by reaction of 1 a with (HgC6F4)3. The compounds were characterised by multinuclear NMR spectroscopy as well as single crystal X‐ray diffraction studies. Supporting computational studies highlight the electronic features of the products.
Cover Image
Authors: Dabbiru Venkata A. S. , Manu Emmanuel , Biedenweg Doreen , Nestler Peter , Pires Ricardo H. , Otto Oliver 
Published: February 2023

ON THE COVER: Effect of surface interactions on F‐Actin levels and cell morphology. Confocal microscopy images of tethered HEK293T cells labeled for F‐Actin and the nucleus. The cover image is based on the Research Article “Cell‐surface contacts determine volume and mechanical properties of human embryonic kidney 293 T cells” by Dabbiru, V. A. S., Manu, E., Biedenweg, D., Nestler, P., Pires, R. H., & Otto, O., https://doi.org/10.1002/cm.21735image
Pain catastrophizing and worry about health in generalized anxiety disorder
Authors: Dugas Michel J. , Giguère Marchal Katia , Cormier Stéphanie , Bouchard Stéphane , Gouin Jean‐Philippe , Shafran Roz 
Published: February 2023

AbstractBecause the diagnostic criteria of generalized anxiety disorder (GAD) are not tied to specific worry domains (worry is ‘generalized’), research on the content of worry in GAD is lacking. To our knowledge, no study has addressed vulnerability for specific worry topics in GAD. The goal of the current study, a secondary analysis of data from a clinical trial, is to explore the relationship between pain catastrophizing and worry about health in a sample of 60 adults with primary GAD. All data for this study were collected at pretest, prior to randomization to experimental condition in the larger trial. The hypotheses were that (1) pain catastrophizing would be positively related to the severity of GAD, (2) the relationship between pain catastrophizing and the severity of GAD would not be explained by intolerance of uncertainty and psychological rigidity, and (3) pain catastrophizing would be greater in participants reporting worry about health compared to those not reporting worry about health. All hypotheses were confirmed, suggesting that pain catastrophizing may be a threat‐specific vulnerability for health‐related worry in GAD. The implications of the current findings include a better understanding of the ideographic content of worry, which could help focus treatment interventions for individuals with GAD.
Generation of Mammalian Cells Devoid of Mitochondrial DNA (ρ0 cells)
Authors: Khozhukhar Natalya , Spadafora Domenico , Rodriguez Yelitza A. Rodriguez , Fayzulin Rafik , Alexeyev Mikhail 
Published: February 2023

AbstractTo cope with DNA damage, mitochondria have developed a pathway whereby severely damaged or unrepairable mitochondrial DNA (mtDNA) molecules can be discarded and degraded, after which new molecules are synthesized using intact templates. In this unit, we describe a method that harnesses this pathway to eliminate mtDNA from mammalian cells by transiently overexpressing the Y147A mutant of human uracil‐N‐glycosylase (mUNG1) in mitochondria. We also provide alternate protocols for mtDNA elimination using either combined treatment with ethidium bromide (EtBr) and dideoxycytidine (ddC) or clustered regulatory interspersed short palindromic repeat (CRISPR)‐Cas9‐mediated knockout of TFAM or other genes essential for mtDNA replication. Support protocols detail approaches for several processes: (1) genotyping ρ0 cells of human, mouse, and rat origin by polymerase chain reaction (PCR); (2) quantification of mtDNA by quantitative PCR (qPCR); (3) preparation of calibrator plasmids for mtDNA quantification; and (4) quantification of mtDNA by direct droplet digital PCR (dddPCR). © 2023 Wiley Periodicals LLC.Basic Protocol: Inducing mtDNA loss with mUNG1Alternate Protocol 1: Generation of ρ0 cells by mtDNA depletion with EtBr and ddCAlternate Protocol 2: Generation of ρ0 cells by knocking out genes critical for mtDNA replicationSupport Protocol 1: Genotyping ρ0 cells by DirectPCRSupport Protocol 2: Determination of mtDNA copy number by qPCRSupport Protocol 3: Preparation of calibrator plasmid for qPCRSupport Protocol 4: Determination of mtCN by direct droplet digital PCR (dddPCR)
Making Bioinformatics Training Events and Material More Discoverable Using TeSS, the ELIXIR Training Portal
Authors: Bacall Finn , Apaolaza Aitor , Andrabi Munazah , Child Chris , Goble Carole , Sand Olivier , Botzki Alexander 
Published: February 2023

AbstractMany trainers and organizations are passionate about sharing their training material. Sharing training material has several benefits, such as providing a record of recognition as an author, offering inspiration to other trainers, enabling researchers to discover training resources for their personal learning path, and improving the training resource landscape using data‐driven gap analysis from the bioinformatics community. In this article, we present a series of protocols for using the ELIXIR online training registry Training eSupport System (TeSS). TeSS provides a one‐stop shop for trainers and trainees to discover online information and content, including training materials, events, and interactive tutorials. For trainees, we provide protocols for registering and logging in and for searching and filtering content. For trainers and organizations, we also show how to manually or automatically register training events and materials. Following these protocols will contribute to promoting training events and add to a growing catalog of materials. This will concomitantly increase the FAIRness of training materials and events. Training registries like TeSS use a scraping mechanism to aggregate training resources from many providers when they have been annotated using Bioschemas specifications. Finally, we describe how to enrich training resources to allow for more efficient sharing of the structured metadata, such as prerequisites, target audience, and learning outcomes using Bioschemas specification. As increasing training events and material are aggregated in TeSS, searching the registry for specific events and materials becomes crucial. © 2023 The Authors. Current Protocols published by Wiley Periodicals LLC.Basic Protocol 1: Searching for training events and materials in TeSSSupport Protocol: Integrating TeSS widgets on your websiteBasic Protocol 2: Logging in to TeSS using an institutional accountAlternate Protocol: Creating and logging in to a TeSS accountBasic Protocol 3: Manual registration of training events in TeSSBasic Protocol 4: Manual registration of training materials in TeSSBasic Protocol 5: Registration of a content provider in TeSSBasic Protocol 6: Automated harvesting of training events and materials in TeSS
Persistent decadal differences in plant communities assembled under contrasting climate conditions
Authors: Eckhoff Kathryn D. , Scott Drew A. , Manning George , Baer Sara G. 
Published: February 2023

AbstractPlant community assembly outcomes can be contingent upon establishment year (year effects) due to variations in the environment. Stochastic events such as interannual variability in climate, particularly in the first year of community assembly, contribute to unpredictable community outcomes over the short term, but less is known about whether year effects produce transient or persistent states on a decadal timescale. To test for short‐term (5‐year) and persistent (decadal) effects of establishment year climate on community assembly outcomes, we restored prairie in an agricultural field using the same methods in four different years (2010, 2012, 2014, and 2016) that captured a wide range of initial (planting) year climate conditions. Species composition was measured for 5 years in all four restored prairies and for 9 and 11 years in the two oldest restored prairies established under average precipitation and extreme drought conditions. The composition of the four assembled communities showed large and significant differences in the first year of restoration, followed by dynamic change over time along a similar trajectory due to a temporary flush of annual volunteer species. Sown perennial species eventually came to dominate all communities, but communities remained distinct from each other in year five. Precipitation in June and July of the establishment year explained short‐term coarse community metrics (i.e., species richness and grass/forb cover), with wet establishment years resulting in a higher cover of grasses and dry establishment years resulting in a higher cover of forbs in restored communities. Short‐term differences in community composition, species richness, and grass/forb cover in restorations established under average precipitation and drought conditions persisted for 9–11 years, with low interannual variability in the composition of each prairie over the long term, indicating persistently different states on a decadal timescale. Thus, year effects resulting from stochastic variation in climate can have decadal effects on community assembly outcomes.
Social network analysis of white‐tailed deer scraping behavior: Implications for disease transmission
Authors: Egan Michael E. , Pepin Kim M. , Fischer Justin W. , Hygnstrom Scott E. , VerCauteren Kurt C. , Bastille‐Rousseau Guillaume 
Published: February 2023

AbstractHost contact structure affects pathogen transmission in host populations, but many measures of host contact do not distinguish contacts that are relevant to pathogen transmission from those that are not. Scrapes are sites for chemical communication by white‐tailed deer (Odocoileus virginianus) during the breeding season and potential sites of transmission of prions, the causative agent of chronic wasting disease (CWD). Scrape‐related behaviors vary in their probability of transmitting prions to or from the environment, suggesting that behavior be combined with contact structure to better reflect potential heterogeneity in prion transmission at scrapes. We recorded visits and behaviors by deer at scrapes throughout DeSoto National Wildlife Refuge, Nebraska in 2005 and 2006. We recorded 2013 interactions by 169 unique identifiable males and 75 females. Adult males performed the most scrape‐related behaviors and spent the most time at scrapes, especially smelling the overhanging branch (70%), smelling the scrape (59%), licking/grasping the overhanging branch (44%), and scraping (36%). We used social network analysis to test the effect of behavior on indirect contact networks among deer at scrapes. By weighting edges based on the frequency and duration of behaviors, we produced networks representing sources of variation in scrape use and compared these networks to evaluate the effects of behavior on network contact structure. Social networks based on scrape‐related behavior were highly connected and dependent upon the frequency, duration, and type of behavior exhibited at scrapes (e.g., scraping, interacting with a scrape or overhanging branch, rub‐urinating, grazing) as well as the age of the deer. Accounting for contact frequency produced networks with lower variation in contact, but higher ability to facilitate contact among disparate groups. Including behavior when defining edges did not preserve the network properties of simpler measures (i.e., unweighted networks) suggesting that heterogeneity in behaviors that affect transmission probability is important for inferring transmission networks from contact networks. High connectivity through indirect contacts suggests that scrapes may be effective targets for management. Adult male deer had the highest connectivity, suggesting that management strategies focused on reducing their interaction with scrapes through density reduction or behavioral modification could reduce the connectivity of indirect contact networks.
Enhancing nutritional quality of spray‐dried concentrated watermelon juice using watermelon by‐product carrier blends
Authors: Milczarek Rebecca R. , Sedej Ivana 
Published: February 2023

AbstractBlends of watermelon (Citrullus lanatus) by‐products (freeze‐dried and ground into powders) and maltodextrin were used as carriers for the pilot‐scale spray drying of preconcentrated watermelon juice. Watermelon juice was first concentrated to 65 °Brix via forward osmosis and then diluted to 16 °Brix. The concentrate was mixed with 25% w/w carrier using one of five carrier blends: 100% maltodextrin, 11% rind (89% maltodextrin), 11% pomace (89% maltodextrin), 25% rind (75% maltodextrin), and 25% pomace (75% maltodextrin). The powders were analyzed for moisture content, water activity, color, true density, bulk density, lycopene and citrulline contents, and total soluble phenolics and antioxidant activity Results indicated that carrier composition had a significant (p < 0.05) effect on all the quality metrics. Powders with any amount of by‐product carrier had higher a* color value than the 100% maltodextrin control. All of the by‐product carrier blends conferred an increase of at least fivefold in lycopene, 1.5‐fold in citrulline, and 1.3‐fold in total soluble phenolics, compared to the all‐maltodextrin carrier. The results of this work will inform the development of new environmentally‐sustainable processing strategies for watermelon juice powder.