bluuebunny/crossref_metadata_2025_split huggingface dataset:
Insight into the Evolution Track for the Metathesis of Alkenes within Hierarchical Zeolite‐Based Catalysts
Authors: Wei Ning , Wang Lizhuo , Yang Wenjie , Zhang Dazhi , Jia Zhenghao , Liu Wei , Zhang Weiping , Zang Jiazhong , Huang Shengjun , Huang Jun 
Published: March 2023

AbstractThe usage of hierarchical MFI zeolite enables a boost of the catalytic performance of Mo‐based catalysts for the olefin‐metathesis reaction. The harvest of active catalysts roots in a segmental evolution track between hierarchical zeolite and Al2O3slices for the fabrication of active sites. The working evolution track requires the indispensable engagements from intracrystalline mesoporous surface, Al2O3slices, and zeolitic Brønsted acid sites. The infilling of disaggregated Al2O3slices into the intracrystalline mesopores triggers the creation of localized intrazeolite−Al2O3interfaces, which enables the subsequent migration and trapping of surface molybdates into the micropores. The insulation of intrazeolite−Al2O3interface or shielding of zeolitic Brønsted acid sites leads to the break of the evolution track. Our findings disclose the hidden functionality of mesoporosity as intrazeolite interface boundary for the fabrication of active sites and supply a new strategy for the rational design of zeolite catalysts.
Structure‐Property Relationships in a New Family of Photoactive Diimine‐Diphosphine Copper(I) Complexes
Authors: Beaudelot Jérôme , Evano Gwilherm , Moucheron Cécile 
Published: March 2023

AbstractA new family of heteroleptic diimine‐diphosphine copper(I) complexes is reported, with six new complexes compared to benchmark [Cu(bcp)(DPEPhos)]PF6. These new complexes are based on 1,4,5,8‐tetraazaphenanthrene (TAP) ligands with representative electronic properties as well as substitution patterns and DPEPhos and XantPhos as diphosphine ligands. Their photophysical and electrochemical properties were investigated and correlated with the number and position of substituents on the TAP ligands. Stern‐Volmer studies using Hünig's base as reductive quencher demonstrated the influence of the complex photoreduction potential and of the excited state lifetime on the photoreactivity. This study refines the structure‐property relationship profile for heteroleptic copper(I) complexes and confirms that such profiles are of high interest to design new copper complexes as optimized photoredox catalysts.
Real‐Time Monitoring of Dynamic Chemical Processes in Microbial Metabolism with Optical Sensors
Authors: Chen Na , Cheng Di , He Tianpei , Yuan Quan 
Published: March 2023

AbstractMonitoring microbial metabolism is vital for revealing the mechanism of disease related to microbial metabolism and providing guidance for biomanufacturing processes optimization. However, it remains a grand challenge to offer real‐time insights into microbial metabolism owing to the complex and dynamic process. In this paper, the recent advances and prospects of optical biosensors including the organic, genetic coding and inorganic optical biosensors are briefly described for real‐time monitoring of dynamic microbial metabolism. This paper points out that challenges remain in microbial heterogeneity. We believe that this work will inspire the application of developing new methods for single cell real‐time analysis.
Models of Affective Illness: Chronic Mild Stress in the Rat
Authors: Papp Mariusz , Willner Paul 
Published: March 2023

AbstractThis article describes a chronic mild stress (CMS) model for predicting antidepressant response and investigating mechanisms of antidepressant action in rats. Following exposure to a variety of mild stressors for several weeks, the rats’ behavior is modified in several ways that parallel symptoms of depression. Among these is a substantial reduction in consumption of a 1% sucrose solution, which models the cardinal symptom of major depression, anhedonia. Our standard procedure employs a battery of behavioral tests, comprising weekly assessment of sucrose intake and, at the end of treatment, the elevated plus‐maze and novel object recognition tests to assess the anxiogenic and dyscognitive effects of CMS. Chronic administration of antidepressant drugs reverses the decreased sucrose intake and other behavioral changes in these subjects. Also effective are second‐generation antipsychotics. The CMS model can be employed in discovery programs to identify anti‐anhedonic drugs (e.g., antidepressants and antipsychotics) that act more quickly than existing agents. While most antidepressants require 3 to 5 weeks to normalize behavior, some treatments provide a faster onset of action. For example, the CMS‐induced deficits can be reversed by acute or sub‐chronic application of treatments that act rapidly in depressed patients, such as deep brain stimulation (DBS), ketamine, and scopolamine, as well as several compounds that have yet to be tested in humans but have fast‐onset antidepressant‐like effects in animals, such as the 5‐HT‐1A biased agonists NLX‐101 and GLYX‐13. Application of the CMS model in Wistar‐Kyoto (WKY) rats causes similar behavioral changes to those seen in Wistars, but these are not reversed by antidepressant treatment. However, WKY rats respond to DBS and ketamine, which are effective in patients who are antidepressant non‐responders, establishing CMS in WKY rats as a model of treatment‐resistant depression. © 2023 The Authors. Current Protocols published by Wiley Periodicals LLC.Basic Protocol: Induction of chronic mild stress in rats as a model of depression and treatment‐resistant depression
Cytology of micropapillary mucinous carcinoma: A case report of a clinically and genetically distinct breast carcinoma variant
Authors: Pradeep Immanuel , Nigam Jitendra Singh , Rathod Gunvanti , Parmeshwar Tushar Mohanlal 
Published: March 2023

AbstractMicropapillary mucinous carcinoma (MPMC) is an uncommon histopathological variant of breast cancer accounting for approximately one‐fifth of all mucinous breast carcinomas. In contrast to pure mucinous carcinoma, MPMC tends to affect younger women and is associated with decreased progression‐free survival, higher nuclear grade, lymphovascular invasion, lymph node metastasis, and positive HER2 status. Typically MPMC histology shows micropapillary architecture with “hobnailing” of cells and reverse polarity. Very few publications document the cytomorphological findings of MPMC. We report a case of MPMC that was suspected in fine needle aspiration cytology (FNAC) and confirmed at histopathology.
Multiscale phenological niches of seed fall in diverse Amazonian plant communities
Authors: Pak Damie , Swamy Varun , Alvarez‐Loayza Patricia , Cornejo‐Valverde Fernando , Queenborough Simon A. , Metz Margaret R. , Terborgh John , Valencia Renato , Wright S. Joseph , Garwood Nancy C. , Lasky Jesse R. 
Published: March 2023

AbstractPhenology has long been hypothesized as an avenue for niche partitioning or interspecific facilitation, both promoting species coexistence. Tropical plant communities exhibit striking diversity in reproductive phenology, but many are also noted for large synchronous reproductive events. Here we study whether the phenology of seed fall in such communities is nonrandom, the temporal scales of phenological patterns, and ecological factors that drive reproductive phenology. We applied multivariate wavelet analysis to test for phenological synchrony versus compensatory dynamics (i.e., antisynchronous patterns where one species' decline is compensated by the rise of another) among species and across temporal scales. We used data from long‐term seed rain monitoring of hyperdiverse plant communities in the western Amazon. We found significant synchronous whole‐community phenology at multiple timescales, consistent with shared environmental responses or positive interactions among species. We also observed both compensatory and synchronous phenology within groups of species (confamilials) likely to share traits and seed dispersal mechanisms. Wind‐dispersed species exhibited significant synchrony at ~6‐month scales, suggesting these species might share phenological niches to match the seasonality of wind. Our results suggest that community phenology is shaped by shared environmental responses but that the diversity of tropical plant phenology may partly result from temporal niche partitioning. The scale‐specificity and time‐localized nature of community phenology patterns highlights the importance of multiple and shifting drivers of phenology.
Serum angiotensin‐converting enzyme levels indicating early sarcoidosis diagnosis and immunosuppressive therapy efficacy
Authors: Kawai Hideki , Naruse Hiroyuki , Sarai Masayoshi , Kato Yasuchika , Sato Yoshihiro , Takahashi Hiroshi , Ishii Junnichi , Ito Hiroyasu , Morimoto Shin‐ichiro , Izawa Hideo 
Published: March 2023

AbstractAimsThis study aimed to determine the new cut‐off value of serum angiotensin‐converting enzyme (ACE) levels for detecting patients with sarcoidosis and to examine the change in ACE levels after the initiation of immunosuppressive therapy.Methods and resultsWe retrospectively examined patients in whom serum ACE levels were measured for suspected sarcoidosis between 2009 and 2020 in our institution. For patients diagnosed with sarcoidosis, changes in ACE levels were also observed. Of the 3781 patients (51.1% men, 60.1 ± 17.0 years old), 477 were excluded for taking ACE inhibitors and/or immunosuppression agents or those with any diseases affecting serum ACE levels. In 3304 patients including 215 with sarcoidosis, serum ACE levels were 19.6 IU/L [interquartile range, 15.1–31.5] in patients with sarcoidosis and 10.7 [8.4–16.5] in those without sarcoidosis (P < 0.01), and the best cut‐off value was 14.7 IU/L with 0.865 of the area under the curves. Compared with the current ACE cut‐off of 21.4, the sensitivity improved from 42.3 to 78.1 at the new cut‐off, although specificity slightly decreased from 98.6 to 81.7. The ACE level significantly decreased more in those with immunosuppression therapy than in those without it (P for interaction <0.01), although it decreased in both groups (P < 0.01).ConclusionsBecause the sensitivity for detecting sarcoidosis is comparatively low at the current standard value, further examinations are needed for patients suspected of sarcoidosis with relatively high ACE levels in the normal range. In patients with sarcoidosis, ACE levels decreased after the initiation of immunosuppression therapy.
Ideologically‐based contact avoidance during a pandemic: Blunt or selective distancing from ‘others’?
Authors: Hodson Gordon , Meleady Rose 
Published: March 2023

AbstractThis project sought to understand when ideology is relevant (or not) to predicting contact avoidance of ‘others’ during the COVID‐19 pandemic. Right‐leaning ideologies (political conservatism, right‐wing authoritarianism, social dominance orientation) were not expected to predict greater contact avoidance per se, but rather exhibit selective avoidance of outgroup (vs. ingroup) members. White British participated in one exploratory (Study 1 N = 364) and two pre‐registered (Study 2 N = 431, Study 3 N = 700) studies. As expected, right‐leaning ideologies were significantly stronger predictors of greater preferred personal distance and contact discomfort regarding foreign outgroups (vs. British ingroup) in Studies 1 and 3 (partially supported in Study 2). Ideology rarely predicted ingroup reactions. This Ideology × Target pattern was itself not moderated by the perceived COVID‐19 threat. Pre‐pandemic theorizing that heightened behavioural immune system responses are associated with heightened right‐leaning ideologies appear insufficient for use in actual pandemic contexts, especially when highly politicized.
Evaluation of the accuracy of hTERT gene aberrant methylation using electrochemical hybridization assay and liquid‐based cytology in screening for oral squamous cell carcinoma
Authors: Haraguchi Kazuya , Sato Shinobu , Habu Manabu , Yada Naomi , Hayakawa Mana , Sasaguri Masaaki , Yoshioka Izumi , Tominaga Kazuhiro , Takenaka Shigeori 
Published: March 2023

AbstractIn order to improve the survival rate of oral squamous cell carcinoma (OSCC) patients, a reliable diagnostic method for early OSCC detection is required that is minimally invasive, less burdensome to the patient, and has high sensitivity and specificity. Therefore, we performed the detection of abnormal methylation at three locations in the hTERT promoter region of oral exfoliated cells by employing the ferrocenylnaphthalene diimide (FND)‐based electrochemical hybridization assay (FND‐EHA) using three types of DNA probe‐immobilized electrodes. We also performed liquid cytology using oral exfoliated cells and compared these obtained data to evaluate whether FND‐ EHA can be used as an OSCC screening system. The results showed a good correlation between this method and conventional OSCC screening, and cytology. In addition, FND‐EHA was also able to determine samples that had been ambiguously determined by liquid cytology. This indicates that FND‐EHA may be useful as an OSCC screening system.
Next‐generation liquid biopsy instruments: Challenges and opportunities
Authors: Zhu Shu , Fang Yaohui , Guo Kefan , Ni Zhonghua , Xiang Nan 
Published: March 2023

AbstractConventional cancer diagnosis needs to excise diseased tissue from the patient's body for biopsy, causing severe injury to patients. Liquid biopsy (LB), with the superior advantage of minimal invasiveness, has shown its ability to cancer diagnosis in real‐time and has been developing promising diagnostic instruments. However, until today, the developed instrument still cannot be an alternative to tissue biopsy in the majority of research and clinical settings. In this paper, we first summarize the challenges and limitations suffered by the existing LB instrument. Then, the opportunities and future progression of the next‐generation instrument are discussed in detail. In all, we hope that the future LB instrument can be eventually integrated into the clinical workflow and serve as a validated and reliable tool for cancer diagnosis.
Catalytic activity of glucose oxidase after dielectrophoretic immobilization on nanoelectrodes
Authors: Prüfer Mareike , Stanke Sandra , Bier Frank F. , Hölzel Ralph 
Published: March 2023

AbstractDielectrophoresis (DEP) is an AC electrokinetic effect that is proven to be effective for the immobilization of not only cells, but also of macromolecules, for example, antibodies and enzyme molecules. In our previous work, we have already demonstrated the high catalytic activity of immobilized horseradish peroxidase after DEP. To evaluate the suitability of the immobilization method for sensing or research in general, we want to test it for other enzymes, too. In this study, glucose oxidase (GOX) from Aspergillus niger was immobilized on TiN nanoelectrode arrays by DEP. Fluorescence microscopy showed the intrinsic fluorescence of the immobilized enzymes flavin cofactor on the electrodes. The catalytic activity of immobilized GOX was detectable, but a fraction of less than 1.3% of the maximum activity that was expected for a full monolayer of immobilized enzymes on all electrodes was stable for multiple measurement cycles. Therefore, the effect of DEP immobilization on the catalytic activity strongly depends on the used enzyme.
Implementation of ubiquitous chromatin opening elements as artificial integration sites for CRISPR/Cas9‐mediated knock‐in in mammalian cells
Authors: Kim Seul Mi , Lee Jaejin , Lee Jae Seong 
Published: March 2023

AbstractCRISPR/Cas9‐mediated targeted gene integration (TI) has been used to generate recombinant mammalian cell lines with predictable transgene expression. Identifying genomic hot spots that render high and stable transgene expression and knock‐in (KI) efficiency is critical for fully implementing TI‐mediated cell line development (CLD); however, such identification is cumbersome. In this study, we developed an artificial KI construct that can be used as a hot spot at different genomic loci. The ubiquitous chromatin opening element (UCOE) was employed because of its ability to open chromatin and enable stable and site‐independent transgene expression. UCOE KI cassettes were randomly integrated into CHO‐K1 and HEK293T cells, followed by TI of enhanced green fluorescent protein (EGFP) onto the artificial UCOE KI site. The CHO‐K1 random pool harboring 5′2.2A2UCOE‐CMV displayed a significant increase in EGFP expression level and KI efficiency compared with that of the control without UCOE. In addition, 5′2.2A2UCOE‐CMV showed improved Cas9 accessibility in the HEK293T genome, leading to an increase in indel frequency and homology‐independent KI. Overall, this assessment revealed the potential of UCOE KI constructs as artificial integration sites in streamlining the screening of high‐production targeted integrants by mitigating the selection of genomic hot spots.
Surgical factors affecting regionally metastatic cutaneous squamous cell carcinoma to the parotid gland
Authors: Rao Vishwanatha M. , Feng Allen L. , Deschler Daniel G. , Kaufman Howard L. , Lin Derrick T. , Miller David , Richmon Jeremy D. , Shalhout Sophia Z. , Varvares Mark A. , Emerick Kevin S. 
Published: March 2023

AbstractBackgroundUnderstanding the impact of surgical treatment on regionally metastatic cutaneous squamous cell carcinoma (cSCC).MethodsRetrospective series of 145 patients undergoing parotidectomy and neck dissection for regionally metastatic cSCC to the parotid. Overall survival (OS), disease‐specific survival (DSS), and disease‐free survival (DFS) analyzed over 3 years. Multivariate analysis was completed using Cox proportional hazard models.ResultsOS was 74.5%, DSS was 85.5% and DFS was 64.8%. On multivariate analysis, immune status (HR = 3.225[OS], 5.119[DSS], 2.071[DFS]) and lymphovascular invasion (HR = 2.380[OS], 5.237[DSS], 2.595[DFS]) were predictive for OS, DSS, and DFS. Margin status (HR = 2.296[OS], 2.499[DSS]) and ≥18 resected nodes (HR = 0.242[OS], 0.255[DSS]) were predictive of OS and DSS, while adjuvant therapy was predictive of DSS (p = 0.018).ConclusionsImmunosuppression and lymphovascular invasion portended worse outcomes in patients with metastatic cSCC to the parotid. Microscopically positive margins and <18 nodes resected are associated with worse OS and DSS, while patients receiving adjuvant therapy had improved DSS.
Ligation of the ethmoid arteries in superselective intra‐arterial infusion of cisplatin for advanced maxillary sinus cancer fed by the ophthalmic artery
Authors: Tamagawa Kotaro , Shinomiya Hirotaka , Okada Takuya , Tatehara Shun , Teshima Masanori , Sasaki Ryohei , Nibu Ken‐ichi 
Published: March 2023

AbstractBackgroundSuperselective cisplatin (CDDP) infusion via the external carotid artery system and concomitant radiotherapy (RADPLAT) provides favorable oncological and functional outcomes in patients with maxillary sinus cancer. However, targeted lesions are occasionally fed by the branch of the internal carotid artery.MethodsIn RADPLAT for maxillary sinus cancer partly fed by the ophthalmic artery, the ethmoid arteries were ligated in two patients without medial orbital wall involvement. In four patients with that, CDDP was administered via the ophthalmic artery.ResultsA complete response was obtained in all six patients. Locoregional recurrence was not observed in any cases. However, visual acuity was lost in four patients who received the ophthalmic artery infusion.ConclusionsLigation of the ethmoid arteries is recommended in RADPLAT for maxillary sinus cancer with lesions fed by the ophthalmic artery. CDDP administered via the ophthalmic artery may be considered if a patient accepts the possibility of visual loss.
Bayesian sample size planning for developmental studies
Authors: Visser Ingmar , Kucharský Šimon , Levelt Claartje , Stefan Angelika M. , Wagenmakers Eric‐Jan , Oakes Lisa 
Published: March 2023

AbstractRunning developmental experiments, particularly with infants, is often time‐consuming and intensive, and the recruitment of participants is hard and expensive. Thus, an important goal for developmental researchers is to optimize sampling plans such that neither too many nor too few participants are tested given the hypothesis of interest. One approach that enables such optimization is the use of Bayesian sequential designs. The use of such sequential designs allows data collection to be terminated as soon as the evidence is deemed sufficiently strong, without compromising the interpretability of the test outcome. In this tutorial, we illustrate how to plan a Bayesian sequential testing design prior to data collection by the method of Bayes factor design analysis—the Bayesian equivalent of power analysis—and discuss the relevance of this for developmental psychologists. The tutorial provides a step‐by‐step guide to perform such analyses, and the methods are illustrated using commonly used statistics in a typical infant‐looking time paradigm such that researchers can easily adapt these methods for their studies.Highlights
Bayesian Sequential Testing can be used to optimize sample sizes and save on data collection.
Bayes Factor Design Analysis can be used to analyze a sequential testing study prior to data collection.
Step‐by‐step guide for performing Bayes Sequential Testing and Bayes Factor Design Analysis.
Seed lost to perineum from hydrogel spacer after brachytherapy for prostate cancer
Authors: Nakiri Makoto , Ueda Kosuke , Hoshino Ryuji , Ogasawara Naoyuki , Kurose Hirofumi , Nishihara Kiyoaki , Muraki Koichiro , Hattori Chikayuki , Ogo Etsuyo , Igawa Tsukasa 
Published: March 2023

IntroductionWe describe a rare case of brachytherapy for prostate cancer in which a seed was lost from the perineum after a hydrogel injection.Case presentationA 71‐year‐old Japanese man was diagnosed with localized high‐risk prostate cancer. Trimodality therapy with I‐125 brachytherapy was selected, and combined androgen blockade therapy was initiated. Brachytherapy and hydrogel injection were performed 7 months after combined androgen blockade initiation; 6 months later, the patient visited our hospital with complaints of redness and bleeding in the perineum. Serous effusion and loss of a seed on the right side of the perineal anus were observed. Pelvic magnetic resonance imaging showed a tunnel like discharge of hydrogel from the dorsal prostate to the perineum. The fistula was incised, the seed was removed, and drainage was performed.ConclusionAppropriate diagnosis and treatment with careful follow‐up are required in patients at high risk of infection after brachytherapy with hydrogel injection.