bluuebunny/crossref_metadata_2025_split huggingface dataset:
In Vivo Assessment of the Apatite-Forming Ability of Second-Generation Hydraulic Calcium Silicate Cements Using a Rat Subcutaneous Implantation Model
Authors: Edanami Naoki , Takenaka Shoji , Belal Razi Saifullah Ibn , Yoshiba Kunihiko , Takahara Shintaro , Yoshiba Nagako , Ohkura Naoto , Noiri Yuichiro 
Published: February 2023

Abstract
Hydroxyapatite formation on endodontic hydraulic calcium silicate cements (HCSCs) plays a significant role in sealing the root canal system and elevating the hard-tissue inductivity of the materials. This study aimed to evaluate the in vivo apatite-forming ability of 13 second-generation HCSCs using a representative first-generation HCSC (white ProRoot MTA: PR) as a positive control. Thirteen second-generation HCSCs and PR were loaded into polytetrafluoroethylene tubes and implanted in subcutaneous tissue of 4-week-old male Wistar rats. At 28 days after implantation, hydroxyapatite formation on the HCSC implants was assessed with micro-Raman spectroscopy, surface ultrastructural and elemental characterization, and elemental mapping of the material–tissue interface. A Raman band for hydroxyapatite (v1 PO43- band at 960 cm−t) and hydroxyapatite-like calcium-phosphorus-rich spherical precipitates were detected on six second-generation HCSCs and PR. In the elemental mapping, calcium-phosphorus-rich hydroxyapatite-layer-like regions were not observed on the seven HCSCs that showed neither the hydroxyapatite Raman band nor hydroxyapatite-like spherical precipitates. These results indicated that only 6 of the 13 second-generation HCSCs produced a detectable amount of hydroxyapatite in rat subcutaneous tissue within 28 days, similar to PR. The seven second-generation HCSCs that did not exhibit hydroxyapatite formation may not be suitable alternatives to PR due to their weak in vivo apatite-forming ability.
Risk assessment of floor water inrush in a coal seam based on an improved variable-weight mode
Authors: Wu Ya-zun , Wang Zhi-Qiang , Lin Yun , Wu Peng , Liu Yuan , Wang Xue-Qi 
Published: February 2023

Abstract
In solving a coal-seam floor water-inrush risk evaluation, the fixed weight in the traditional constant-weight model cannot clearly reflect the change of the index value of the main control factors and the combined relationship under the sudden change. In this paper, the improved variable-weight model was introduced into the risk assessment of floor water inrush. The variable-weight interval of the state variable-weight function was adjusted into three sections: the penalty interval, no-penalty no-incentive interval, and incentive interval. The variable-weight interval threshold values were determined by the cumulative frequency of the standardized values of each factor. The penalty interval threshold value dj1 was determined with 25% of the cumulative frequency as the limit. The threshold value dj2 of the no-penalty and no-incentive interval was determined by taking 75% of the cumulative frequency as the limit. According to the variation law of water-inrush prediction and evaluation of the coal-seam floor, the zonal state variable-weight vector and the improved variable-weight model were constructed. At the same time, the zonal variable-weight model and the traditional constant-weight model were used to predict and evaluate the water-inrush risk of the #5 coal seam in the Panjiayao mine field (Datong City, Shanxi Province, China). The results showed that the variable-weight model had better prediction and evaluation effects, and it was more in-line with the reality of the situation.
The myo-inositol biosynthesis rate-limiting enzyme ISYNA1 suppress the stemness of ovarian cancer
Authors: Yang Lingling , Yang Muyao , Cui Chenxi , Long Xingtao , Li Yunzhe , Lang Tingyuan , Zhou Qi 
Published: February 2023

AbstractCancer stem cells (CSCs) play a central role in tumor formation, invasion, metastasis, chemoresistance and relapse of ovarian cancer (OC). Here we report myo-inositol biosynthesis rate-limiting enzyme (ISYNA1), as a suppressor of OC and regulate cancer stem-like properties of ovarian cancer cells. The Cancer Genome Atlas (TCGA) database and Gene Expression Omnibus (GEO) database results showed that lower ISYNA1 level was correlated with shorter overall survival (OS) in TCGA and GEO database. In GEO database, cancer tissues expressed lower level of ISYNA1 compared to normal tissues, and negative associated with the stemness markers. ISYNA1 deficiency promoting the growth, migration, invasion, and self-renewal capability of ovarian cancer cells. We also found that silencing ISYNA1 in ovarian cancer cells enhance CSC properties including sphere formation, the expression level of stem cell factors such as SOX2, NANOG and OCT4, as well as the expression of CSC markers CD44 and CD133, besides, the aldehyde dehydrogenase (ALDH) activity, CD44+/CD117+expression also be analyzed. In contrast, ectopic overexpression of ISYNA1 suppress cell proliferation, migration, and invasion, decrease the stemness of ovarian cancer cells. In xenograft models, ISYNA1 deficiency significantly increases the tumorgenicity and CSC frequency. In addition, we identified P53 could regulate the expression of ISYNA1, and the myo-inositol addition rescue the stemness increased by ISYNA1 knockdown. In conclusion, these data establish the evidence of ISYNA1 as a suppressor of OC and a regulator of OCSCs, providing insight into potentially targetable pathways for ovarian cancer therapy.
Tissue-specific cells generated to predict xenogeneic immune responses demonstrate that SLA-downregulated kidney proximal tubular epithelial cells are low immunogenic
Authors: Schmalkuche Katharina , Schwinzer Reinhard , Wenzel Nadine , Valdivia Emilio , Petersen Björn , Blasczyk Rainer , Figueiredo Constanca 
Published: February 2023

Abstract
Patients with kidney failure depend on transplantation as the only curative option. Xenotransplantation re-emerged as a promising alternative to enlarge the available organ pool. However, the success of xenotransplantation depends on the design and selection of specific genetic modifications and on the development of robust assays allowing for a precise assessment of tissue-specific immune responses. Nevertheless, cell-based assays are often compromised by the low proliferative capacity of primary cells. Proximal tubular epithelial cells (PTECs) play a crucial role in kidney function. Here, we immortalized PTEC (imPTEC) by overexpression of simian virus 40 T large antigen. imPTEC showed typical morphology, phenotype, and functionality, but maintained steady cell cycling rates. Furthermore, SLA class I and class II transcript levels were reduced by up to 85% after transduction with lentiviral vectors encoding for shRNAs targeting β2-microglobulin and the class II transactivator. This contributed to reduce xenogeneic T-cell cytotoxicity (P = 0.0069) and decrease pro-inflammatory cytokine secretion such as IL-6 and IFN-γ. This study showed the feasibility to generate highly proliferative renal tubular cells and the development of tissue-specific immunomonitoring assays. Silencing SLA expression on PTEC demonstrated to be an effective strategy to prevent xenogeneic cellular immune responses and may strongly support graft survival after xenotransplantation.
Perioperative remedial antiviral therapy in HBV-related HCC resection: how to get a better outcome
Authors: Mu Fan , Zhao Zhen , Xu Kun , Guo Kun , Shi Jian-hua , Lu Lu , Ma Lei , Lv Yi , Hu Liang-shuo , Wang Bo 
Published: February 2023

AbstractBackground:Although the benefits of antiviral therapy against hepatitis B virus (HBV)-related hepatocellular carcinoma (HCC) have been proven, researchers have not confirmed the different outcomes between patients who had received a period of time (at least 24 weeks) or just received remedial antiviral therapy before radical resection for HBV-related HCC.Methods:A retrospective study of patients who underwent radical resection for HBV-related HCC at the First Affiliated Hospital of Xi'an Jiaotong University from January 2016 to June 2019 was conducted. Considering the history of antiviral therapy, patients were divided into remedial antiviral therapy and preoperative antiviral therapy groups.Results:The Kaplan–Meier analysis showed a significant difference in overall survival (P<0.0001) and disease-free survival (P=0.035) between the two groups. Multivariate analysis demonstrated that history of preoperative antiviral treatment was independently related to enhanced survival outcome in OS (HR 0.27; 95% CI= 0.08–0.88, P=0.030).Conclusions:In patients with HBV-related HCC, it is ideal to receive preoperative long-term antiviral therapy which help patients tolerate more extensive hepatectomy, but remedial antiviral therapy reducing preoperative HBV-DNA levels to less than 4 log10 copies DNA/ml can also resulted in enhanced outcomes.
Physiological-Biochemical and Molecular Change in Triticum aestivum L. Exposed to Lead Stress
Authors: Taspinar Mahmut Sinan , Kuloglu Selin Sipahi , Aydin Murat , Agar Guleray 
Published: February 2023

Abstract
Lead (Pb) is one of the most hazardous heavy metals that generate environmental pollution because it spreads as metal or compound into the atmosphere and is toxic in all cases. Pb is nondegradable, it can easily enter the food chain and endanger the health of humans and animals. It is unavoidable to investigate the protective mechanisms and toxicity of lead which is very harmful to plant such as genetic, physiological, and biochemical changes. In this study, the negative effects of Pb to the metallothionein (MT) genes expression and also recovery effects of copper (Cu), zinc (Zn), and salicylic acid (SA) against lead defects were analyzed by the qRT-PCR technique in heavy metal stress-sensitive wheat variety. It has been determined that the MT gene expression changes occur according to the organs and also in recovering applications positively affect these expressions. In addition, research founds confirmed that physiological (relative water content, chlorophyll content, and cell membrane damage) and biochemical changes catalase (CAT), malondialdehyde (MDA), peroxidase (POD), and superoxide dismutase (SOD) content) have been seen on lead stress condition and also SA, Cu and Zn applications have recovering effects. In the case of stress, SA, Cu, and Zn applications have been revealed to play a role in reducing toxicity by altering MT genes expressions and enzyme activities.
Effectiveness of Face Masks in Preventing COVID-19 Transmission in Real-World Settings: A Systematic Literature Review
Authors: Crespo Noe C. , Fornasier Joelle M. , Dionicio Patricia , Godino Job G. , Ramers Christian , Elder John 
Published: February 2023

Abstract
Background. Since its emergence, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has caused an unprecedented global pandemic. Evidence regarding the effectiveness of mask wearing is important to support guidelines for public health practice and implementation of infection-control strategies. Previous systematically reviews within controlled settings suggest wearing face masks are ‘efficacious’ at preventing COVID-19. However, a comprehensive review of the ‘effectiveness’ of face masks in preventing COVID-19 transmission in real-world settings is needed in order to generalize findings. The present study reviewed literature on the effectiveness of wearing face masks in preventing COVID-19 transmission in real-world settings.
Methods. Following PRISMA guidelines, three databases and gray literature sources were searched up until January 2022. Inclusion criteria for studies were: 1) written in or translated into English; 2) assess the SARS-CoV-2 transmission; 3) assess the behavior of mask wearing and 4) study conducted in a non-laboratory-controlled setting (e.g., worksites, schools etc.). All 2,240 search results were reviewed for inclusion. Two reviewers extracted study characteristics. Three reviewers independently assessed quality of evidence and risk of bias using JBI critical appraisal tools.
Results. This review identified 48 unique studies that met the criteria. Most studies investigated the effect of face masks in a healthcare setting (n=21, 43%). The majority (n=44; 91.6%) of studies showed that wearing face masks led to a reduction in transmission or risk of transmission of COVID-19, and the protective effect of masks was increased when combined with other preventive measures. The findings support that face masks may be up to 79% effective in preventing transmission in non-controlled real-life settings.
Conclusions. The results of this systematic review strongly support public health recommendations for wearing a mask to reduce the risk of COVID-19 infection in real-world settings. Effective communication efforts are needed to inform the general public of the benefits of wearing face masks to prevent COVID-19.
Green brand awareness, green brand association, green perceived quality, and intention to purchase electric vehicles: The mediating effect of green trust
Authors: Mombeuil Claudel , Diunugala Hemantha Premakumara 
Published: February 2023

Abstract
As part of their effort to curb CO2 emissions, many big players in the automobile industry have adopted a green branding approach through the design and manufacturing of lightweight electric vehicles. Nevertheless, there are limited studies that address consumers’ intention to purchase these types of vehicles from the perspective of green branding. To fill this gap, this paper examines the direct and indirect effects of green brand awareness, green brand associations, and green perceived quality on consumer intention to purchase electric three-wheelers. A total of 400 useful responses were collected through a questionnaire survey and the hypotheses were tested using structural equation modeling. The results show that all the direct effects were positive and statistically significant. Furthermore, the analysis shows that green trust plays a mediating role in the effects of brand associations and green perceived quality on consumers’ intention to purchase three-wheel electric vehicles from a familiar and trusted brand. Given the potential of electric three-wheelers in curbing CO2 emissions and stakeholders’ skepticism about companies' green branding claims, managers need to give special attention to green trust to better position their green products and stimulate the purchase of these products.
Developing a palmyra palm sacred grove wellness tourism circuit in Tamil Nadu, India involving active stakeholders’ participation
Authors: babu Preethi Hari , Ramasamy Rajesh 
Published: February 2023

Abstract

In Tamil Nadu, palmyra palm is being worshipped by the local community in association with the concepts of tree veneration, sthala vriksham and in connect with tutelary god or local deities (demigods). The related literature has been collected from different key variables on topics such as sacred grove around the globe, myths, taboos and religious beliefs, conservation of sacred grove, importance of palmyra palm, palmyra palm sacred groves, role of wellness tourism circuit and stakeholders’ involvement to sustain and improve the state of sacred grove and local communities. This paper strives to bridge the gap between the concept of palmyra palm sacred grove and misconception of beliefs and practices through detailed Participatory Research Approach. Qualitative data have been collected through unstructured interviews and are analyzed through thematic content analysis tool. The analysis reveals a map portraying potential palmyra palm sacred grove. Subsequently, the study suggests the roles of stakeholders to sustain, conserve and promote palmyra sacred grove as a wellness tourism circuit. Further, the significance of palmyra palm sacred grove, the problems and prospects of promoting palmyra palm sacred grove as a wellness tourism circuit through stakeholder participation are traced to design a framework model.
Development of ATR-FTIR spectroscopy for the diagnosis of Alzheimer’s disease using oral buccal cells
Authors: Paraskevaidi Maria , Karim Salman , Santos Marfran , Lima Kassio , Crean StJohn 
Published: February 2023

Abstract
Background: As general ageing increases, a higher global prevalence of dementia increases in likelihood. Alzheimer’s disease is predicted to triple in cases by 2050, becoming a global concern with heavy impact on socio-economic levels.
Although numerous biomarkers have been explored, their clinical performance, especially in early stages, is limited. Current diagnostic approaches also necessitate the use of invasive procedures or laborious and expensive imaging techniques.
A rapid, low-cost and non-invasive test for the detection of Alzheimer’s disease could be used for the effective identification of individuals that would need referral for further testing. Oral cavity-derived samples, including saliva and buccal mucosal cells, are considered rich sources of biomarkers for Alzheimer’s disease since they can reflect peripheral changes and correlate well with the disease state.
Methods: We assessed the potential of attenuated total reflection-Fourier transform infrared (ATR-FTIR) spectroscopy as a diagnostic tool for Alzheimer’s disease using oral buccal cells.
Findings: After spectroscopic analysis and use of machine learning algorithms, this approach achieved 76% sensitivity and 100% specificity (area under the curve (AUC): 88%) in differentiating patients with Alzheimer’s disease from age-matched healthy controls.
Conclusion: We demonstrate that spectroscopic analysis of buccal cells could detect patients with Alzheimer’s disease with high diagnostic accuracy. Such a test has the potential to provide a non‐invasive, rapid and cost-effective alternative to current CSF and blood sampling procedures. An earlier diagnosis of Alzheimer’s disease and timely intervention are expected to impact on the disease progression.
Early Shunting Promotes Sustained Improvement of Patients with Idiopathic Normal Pressure Hydrocephalus: A Multicenter Retrospective Study
Authors: Xu Hao , He Jiawei , Dong Yongfei , Zhao Kai , Bao Dejun , Wei Xiangpin , Lei Ting , Wu Kang , Wang Zhanxiang , Niu Chaoshi , Liu Xinfeng 
Published: February 2023

Abstract
Background: Idiopathic normal pressure hydrocephalus (iNPH) is the only reversible cause of dementia, but many patients fail to receive shunt surgery in time due to underdiagnosis. This study aimed to determine the relationship between surgery timepoint and sustained postoperative improvement in patients with iNPH.
Methods: We divided the patients into the early surgery group (0–3 months) and the late surgery group (> 3 months) according to the duration from the disease onset to surgery. Patients from 3 general hospitals in different provinces of China were included. Primary endpoints included improvements on the idiopathic normal pressure hydrocephalus grading scale (INPHGS) and modified Rankin Scale (mRS).
Results: A total of 120 patients were included in the study. The mean age was 62.76 ± 12.27, the mean duration of symptoms was 22.00 ± 37.99 months, the mean INPHGS was 7.15 ± 1.759 (cognitive: 2.71 ± 0.91; gait: 2.47 ± 0.95; urinary: 1.96 ± 0.88), and the mean mRS were 2.45 ± 0.77. In the early surgery group, patients’ symptoms improved continuously from discharge to 1-year follow-up. Regarding mRS improvement, the early surgery group is superior to the late surgery group at postoperative time points and 1-year follow-up (p < 0.05).
Conclusions: The results of this multicenter study indicate that early shunting promotes sustained improvement in patients with iNPH.
Relationship between Gut Commensal Bacteria and Response to Neoadjuvant Chemoradiotherapy in Locally Advanced Rectal Cancer
Authors: Chang Pengyu , Gu Meichcen , Xu Tiankai , He Liang , Tong Weihua , Li Meng , Li Shuang , Li Ying , Liu Xinliang , Wang Quan 
Published: February 2023

Abstract
Gut commensal bacteria may have a potential in distorting the response of locally advanced rectal cancer (LARC) to neoadjuvant chemoradiotherapy (nCRT). To this end, fecal samples of 35 LARC patients were retrospectively collected during nCRT in our center. According to pathological tumor regression grade (TRG) classification system (AJCC 8.0), recruited patients were divided into Sensitive group (TRG 0 ~ 1, n = 12) and Resistant group (TRG 2 ~ 3, n = 23) after radical surgery. Fecal bacterial structures of two groups were compared after bacterial 16S rRNA sequencing. In this study, it was found that fecal bacterial structures of two groups before nCRT were significantly different, presenting a higher abundance of butyrate-producing bacteria in feces of Sensitive group, whereas a higher abundance of colorectal cancer-facilitated bacteria in feces of Resistant group. During nCRT, β-diversities of two groups were significantly decreased comparing to their baseline, but bacterial structures of two groups were remodeled differentially, presenting that unlike in Sensitive group, the bacterial structure in Resistant group was continuously evolved as nCRT-dose increased. Intriguingly, it was found that the abundance of Akkermansia.muciniphila, a critical member of probiotics, was positively correlated with tumorigenic bacteria in feces of LARC patients resistant to nCRT, but the bacterial function network analysis revealed that Akkermansia.muciniphila counteracted against tumorigenic bacteria, such as Escherichia and Bacteroides. This highlighted a potential of Akkermansia.muciniphila in improving LARC response to nCRT. Collectively, gut bacterial structure impacts the response of LARC to nCRT. In turn, nCRT will remodel gut bacterial structures among LARC patients, thus enabling their treatment responses to be inconsistent.
The chemokine CXCL7 is correlated with LDH-A and predicts the prognosis of patients with colorectal cancer
Authors: Wang Yin , Li Longhai , Jiang Rongyan , Hao Shuli , Cheng Gang 
Published: February 2023

Abstract

Objective: The aims of this study were to evaluate the correlation between CXCL7 expression and glycolysis and to explore the prognostic significance of CXCL7 in colorectal cancer (CRC).
Methods: The expression of CXCL7 and LDH-A was measured by immunohistochemistry in tissues from 158 CRC patients. All patients were divided into high expression and low expression groups based on receiver operating characteristic curves and a cut-off value. Spearman's rank-correlation analysis was used to evaluate the correlation between CXCL7 and LDH-A expression. Kaplan–Meier analysis and log-rank test were utilized to explore the overall survival (OS) times of CRC patients. A Cox proportional hazard model and nomogram were also used to assess the risk factors related to prognosis.
Results:Higher expression of CXCL7 and LDH-A were detected in CRC tissues and associated with N stage and TNM stage (all P < 0.05). CXCL7 expression was strongly correlated with LDH-A expression in CRC tissues (correlation coefficient [r] = 0.696, P < 0.001). High expression of CXCL7 was validated as an independent risk factor for OS (hazard ratio: 2.066; 95% CI: 1.223–3.490; P = 0.007).
Conclusion: Increased expression of CXCL7 was positively correlated with LDH-A expression and was an independent risk factor for CRC prognosis.