bluuebunny/crossref_metadata_2025_split huggingface dataset:
Carbon mitigation potential afforded by rooftop photovoltaic in China
Authors: Zhang Zhixin , Chen Min , Zhong Teng , Zhu Rui , Qian Zhen , Zhang Fan , Yang Yue , Zhang Kai , Santi Paolo , Wang Kaicun , Pu Yingxia , Tian Lixin , Lü Guonian , Yan Jinyue 
Published: April 2023

AbstractRooftop photovoltaics (RPVs) are crucial in achieving energy transition and climate goals, especially in cities with high building density and substantial energy consumption. Estimating RPV carbon mitigation potential at the city level of an entire large country is challenging given difficulties in assessing rooftop area. Here, using multi-source heterogeneous geospatial data and machine learning regression, we identify a total of 65,962 km2 rooftop area in 2020 for 354 Chinese cities, which represents 4 billion tons of carbon mitigation under ideal assumptions. Considering urban land expansion and power mix transformation, the potential remains at 3-4 billion tons in 2030, when China plans to reach its carbon peak. However, most cities have exploited less than 1% of their potential. We provide analysis of geographical endowment to better support future practice. Our study provides critical insights for targeted RPV development in China and can serve as a foundation for similar work in other countries.
UDP-glucosyltransferase OsUGT75A promotes submergence tolerance during rice seed germination
Authors: He Yongqi , Sun Shan , Zhao Jia , Huang Zhibo , Peng Liling , Huang Chengwei , Tang Zhengbin , Huang Qianqian , Wang Zhoufei 
Published: April 2023

AbstractSubmergence stress represents a major obstacle limiting the application of direct seeding in rice cultivation. Under flooding conditions, coleoptile elongation can function as an escape strategy that contributes to submergence tolerance during seed germination in rice; however, the underlying molecular bases have yet to be fully determined. Herein, we report that natural variation of rice coleoptile length subjected to submergence is determined by the glucosyltransferase encoding gene OsUGT75A. OsUGT75A regulates coleoptile length via decreasing free abscisic acid (ABA) and jasmonic acid (JA) levels by promoting glycosylation of these two phytohormones under submergence. Moreover, we find that OsUGT75A accelerates coleoptile length through mediating the interactions between JASMONATE ZIMDOMAIN (OsJAZ) and ABSCISIC ACID-INSENSITIVE (OsABI) proteins. Last, we reveal the origin of the haplotype that contributes to coleoptile length in response to submergence and transferring this haplotype to indica rice can enhance coleoptile length in submergence conditions. Thus, we propose that OsUGT75A is a useful target in breeding of rice varieties suitable for direct seeding cultivation.
Creasing in microscale, soft static friction
Authors: Glover Justin D. , Yang Xingwei , Long Rong , Pham Jonathan T. 
Published: April 2023

AbstractUtilizing colloidal probe, lateral force microscopy and simultaneous confocal microscopy, combined with finite element analysis, we investigate how a microparticle starts moving laterally on a soft, adhesive surface. We find that the surface can form a self-contacting crease at the leading front, which results from a buildup of compressive stress. Experimentally, creases are observed on substrates that exhibit either high or low adhesion when measured in the normal direction, motivating the use of simulations to consider the role of adhesion energy and interfacial strength. Our simulations illustrate that the interfacial strength plays a dominating role in the nucleation of a crease. After the crease forms, it progresses through the contact zone in a Schallamach wave-like fashion. Interestingly, our results suggest that this Schallamach wave-like motion is facilitated by free slip at the adhesive, self-contacting interface within the crease.
TMEM25 inhibits monomeric EGFR-mediated STAT3 activation in basal state to suppress triple-negative breast cancer progression
Authors: Bi Jing , Wu Zhihui , Zhang Xin , Zeng Taoling , Dai Wanjun , Qiu Ningyuan , Xu Mingfeng , Qiao Yikai , Ke Lang , Zhao Jiayi , Cao Xinyu , Lin Qi , Chen Xiao Lei , Xie Liping , Ouyang Zhong , Guo Jujiang , Zheng Liangkai , Ma Chao , Guo Shiying , Chen Kangmei , Mo Wei , Fu Guo , Zhao Tong-Jin , Wang Hong-Rui 
Published: April 2023

AbstractTriple-negative breast cancer (TNBC) is a subtype of breast cancer with poor outcome and lacks of approved targeted therapy. Overexpression of epidermal growth factor receptor (EGFR) is found in more than 50% TNBC and is suggested as a driving force in progression of TNBC; however, targeting EGFR using antibodies to prevent its dimerization and activation shows no significant benefits for TNBC patients. Here we report that EGFR monomer may activate signal transducer activator of transcription-3 (STAT3) in the absence of transmembrane protein TMEM25, whose expression is frequently decreased in human TNBC. Deficiency of TMEM25 allows EGFR monomer to phosphorylate STAT3 independent of ligand binding, and thus enhances basal STAT3 activation to promote TNBC progression in female mice. Moreover, supplying TMEM25 by adeno-associated virus strongly suppresses STAT3 activation and TNBC progression. Hence, our study reveals a role of monomeric-EGFR/STAT3 signaling pathway in TNBC progression and points out a potential targeted therapy for TNBC.
Femtosecond-precision electronic clock distribution in CMOS chips by injecting frequency comb-extracted photocurrent pulses
Authors: Hyun Minji , Chung Hayun , Na Woongdae , Kim Jungwon 
Published: April 2023

AbstractA clock distribution network (CDN) is a ubiquitous on-chip element that provides synchronized clock signals to all different circuit blocks in the chip. To maximize the chip performance, today’s CDN demands lower jitter, skew, and heat dissipation. Conventionally, on-chip clock signals have been distributed in the electric voltage domain, resulting in increased jitter, skew, and heat dissipation due to clock drivers. While low-jitter optical pulses have been locally injected in the chip, research on effective distribution of such high-quality clock signals has been relatively sparse. Here, we demonstrate femtosecond-precision distribution of electronic clocks using driver-less CDNs injected by photocurrent pulses extracted from an optical frequency comb source. Femtosecond-level on-chip jitter and skew can be achieved for gigahertz-rate clocking of CMOS chips by combining ultralow comb-jitter, multiple driver-less metal-meshes, and active skew control. This work shows the potential of optical frequency combs for distributing high-quality clock signals inside high-performance integrated circuits, including 3D integrated circuits.
Dissecting the conformational complexity and mechanism of a bacterial heme transporter
Authors: Wu Di , Mehdipour Ahmad R. , Finke Franziska , Goojani Hojjat G. , Groh Roan R. , Grund Tamara N. , Reichhart Thomas M. B. , Zimmermann Rita , Welsch Sonja , Bald Dirk , Shepherd Mark , Hummer Gerhard , Safarian Schara 
Published: April 2023

AbstractIron-bound cyclic tetrapyrroles (hemes) are redox-active cofactors in bioenergetic enzymes. However, the mechanisms of heme transport and insertion into respiratory chain complexes remain unclear. Here, we used cellular, biochemical, structural and computational methods to characterize the structure and function of the heterodimeric bacterial ABC transporter CydDC. We provide multi-level evidence that CydDC is a heme transporter required for functional maturation of cytochrome bd, a pharmaceutically relevant drug target. Our systematic single-particle cryogenic-electron microscopy approach combined with atomistic molecular dynamics simulations provides detailed insight into the conformational landscape of CydDC during substrate binding and occlusion. Our simulations reveal that heme binds laterally from the membrane space to the transmembrane region of CydDC, enabled by a highly asymmetrical inward-facing CydDC conformation. During the binding process, heme propionates interact with positively charged residues on the surface and later in the substrate-binding pocket of the transporter, causing the heme orientation to rotate 180°.
Dynamic mitochondrial transcription and translation in B cells control germinal center entry and lymphomagenesis
Authors: Yazicioglu Yavuz F. , Marin Eros , Sandhu Ciaran , Galiani Silvia , Raza Iwan G. A. , Ali Mohammad , Kronsteiner Barbara , Compeer Ewoud B. , Attar Moustafa , Dunachie Susanna J. , Dustin Michael L. , Clarke Alexander J. 
Published: April 2023

AbstractGerminal center (GC) B cells undergo proliferation at very high rates in a hypoxic microenvironment but the cellular processes driving this are incompletely understood. Here we show that the mitochondria of GC B cells are highly dynamic, with significantly upregulated transcription and translation rates associated with the activity of transcription factor A, mitochondrial (TFAM). TFAM, while also necessary for normal B cell development, is required for entry of activated GC precursor B cells into the germinal center reaction; deletion of Tfam significantly impairs GC formation, function and output. Loss of TFAM in B cells compromises the actin cytoskeleton and impairs cellular motility of GC B cells in response to chemokine signaling, leading to their spatial disorganization. We show that B cell lymphoma substantially increases mitochondrial translation and that deletion of Tfam in B cells is protective against the development of lymphoma in a c-Myc transgenic mouse model. Finally, we show that pharmacological inhibition of mitochondrial transcription and translation inhibits growth of GC-derived human lymphoma cells and induces similar defects in the actin cytoskeleton.
Structural insights into thrombolytic activity of destabilase from medicinal leech
Authors: Marin Egor , Kornilov Daniil A. , Bukhdruker Sergey S. , Aleksenko Vladimir A. , Manuvera Valentin A. , Zinovev Egor V. , Kovalev Kirill V. , Shevtsov Mikhail B. , Talyzina Anna A. , Bobrovsky Pavel A. , Kuzmichev Pavel K. , Mishin Alexey V. , Gushchin Ivan Y. , Lazarev Vassili N. , Borshchevskiy Valentin I. 
Published: April 2023

AbstractDestabilase from the medical leech Hirudo medicinalis belongs to the family of i-type lysozymes. It has two different enzymatic activities: microbial cell walls destruction (muramidase activity), and dissolution of the stabilized fibrin (isopeptidase activity). Both activities are known to be inhibited by sodium chloride at near physiological concentrations, but the structural basis remains unknown. Here we present two crystal structures of destabilase, including a 1.1 Å-resolution structure in complex with sodium ion. Our structures reveal the location of sodium ion between Glu34/Asp46 residues, which were previously recognized as a glycosidase active site. While sodium coordination with these amino acids may explain inhibition of the muramidase activity, its influence on previously suggested Ser49/Lys58 isopeptidase activity dyad is unclear. We revise the Ser49/Lys58 hypothesis and compare sequences of i-type lysozymes with confirmed destabilase activity. We suggest that the general base for the isopeptidase activity is His112 rather than Lys58. pKa calculations of these amino acids, assessed through the 1 μs molecular dynamics simulation, confirm the hypothesis. Our findings highlight the ambiguity of destabilase catalytic residues identification and build foundations for further research of structure–activity relationship of isopeptidase activity as well as structure-based protein design for potential anticoagulant drug development.
Early central cardiovagal dysfunction after high fat diet in a murine model
Authors: Strain Misty M. , Espinoza Liliana , Fedorchak Stephanie , Littlejohn Erica L. , Andrade Mary Ann , Toney Glenn M. , Boychuk Carie R. 
Published: April 2023

AbstractHigh fat diet (HFD) promotes cardiovascular disease and blunted cardiac vagal regulation. Temporal onset of loss of cardiac vagal control and its underlying mechanism are presently unclear. We tested our hypothesis that reduced central vagal regulation occurs early after HFD and contributes to poor cardiac regulation using cardiovascular testing paired with pharmacology in mice, molecular biology, and a novel bi-transgenic mouse line. Results show HFD, compared to normal fat diet (NFD), significantly blunted cardio/pulmonary chemoreflex bradycardic responses after 15 days, extending as far as tested (> 30 days). HFD produced resting tachycardia by day 3, reflected significant loss of parasympathetic tone. No differences in bradycardic responses to graded electrical stimulation of the distal cut end of the cervical vagus indicated diet-induced differences in vagal activity were centrally mediated. In nucleus ambiguus (NA), surface expression of δ-subunit containing type A gamma-aminobutyric acid receptors (GABAA(δ)R) increased at day 15 of HFD. Novel mice lacking δ-subunit expression in vagal motor neurons (ChAT-δnull) failed to exhibit blunted reflex bradycardia or resting tachycardia after two weeks of HFD. Thus, reduced parasympathetic output contributes to early HFD-induced HR dysregulation, likely through increased GABAA(δ)Rs. Results underscore need for research on mechanisms of early onset increases in GABAA(δ)R expression and parasympathetic dysfunction after HFD.
LPS induces microglial activation and GABAergic synaptic deficits in the hippocampus accompanied by prolonged cognitive impairment
Authors: Jung Hyeji , Lee Dongsu , You Heejung , Lee Myungha , Kim Hyeonho , Cheong Eunji , Um Ji Won 
Published: April 2023

AbstractNeuroinflammation impacts the brain and cognitive behavior through microglial activation. In this study, we determined the temporal sequence from microglial activation to synaptic dysfunction and cognitive behavior induced by neuroinflammation in mice. We found that LPS injection activated microglia within a short period, followed by impairments in GABAergic synapses, and that these events led to long-term cognitive impairment. We demonstrated that, 3 days after LPS injection, microglia in the hippocampus were significantly activated due to the LPS-induced inflammation in association with alterations in cellular morphology, microglial density, and expression of phagocytic markers. GABAergic synaptic impairments were detected at 4–6 days after LPS treatment, a time when microglia activity had returned to normal. Consequently, memory impairment persisted for 6 days after injection of LPS. Our results suggest that neuroinflammation induces microglia activation, GABAergic synaptic deficits and prolonged memory impairment over a defined temporal sequence. Our observations provide insight into the temporal sequence of neuroinflammation-associated brain pathologies. Moreover, the specific loss of inhibitory synapses accompanying the impaired inhibitory synaptic transmission provides mechanistic insight that may explain the prolonged cognitive deficit observed in patients with neuroinflammation. Thus, this study provides essential clues regarding early intervention strategies against brain pathologies accompanying neuroinflammation.
Influence of rootstock on endogenous hormones and color change in Cabernet Sauvignon grapes
Authors: Liu Zhiyu , Zhu Chunmei , Sun Junli , Zhang Zhijun , Zhao Shucheng , Shi Wenchao , Wang Wei , Zhao Baolong 
Published: April 2023

AbstractDifferent rootstocks for grapes can significantly affect fruit color and quality, possibly by affecting hormone contents, related genetic pathways, and fruit coloring mechanisms in skin. ‘Cabernet Sauvignon’ was grafted to ‘5BB’, ‘SO4’, ‘140R’, ‘CS’, ‘3309M’ and ‘Vitis riparia’ rootstocks, with self-rooting seedlings as the control (CS/CS), and sampled from the early stage of veraison to the ripening stage. The effects of rootstock on the contents of gibberellin (GA3), auxin (IAA), and abscisic acid (ABA) in grape skin were determined alongside the expression levels of eight anthocyanin synthesis related genes using real-time fluorescence quantitative PCR methods. The rootstock cultivars exhibited accelerated fruit color change, and the CS/140R combination resulted in grapes with more color than the control group in the same period. With the development of fruit, the IAA and GA3 contents in the skin of different rootstock combinations showed trends of increasing initially, then decreasing, while the ABA content decreased initially and then increased. During the verasion (28 July), the various ‘Cabernet Sauvignon’ rootstock combinations exhibited varying degrees of increases in GA3, ABA, and IAA contents; correlation analysis showed that, at the start of veraison, the expression levels of the anthocyanin synthesis-related genes VvCHS, VvDFR, and VvUFGT had strong positive correlations with hormone contents, which indicated they are key genes involved in the endogenous hormone responsive anthocyanin biosynthesis pathway. The results of this study showed that rootstock regulates the fruit coloring process by influencing the metabolism level of peel hormones in the ‘Cabernet Sauvignon’ grape.
Genomic profiling of idiopathic peri-hilar cholangiocarcinoma reveals new targets and mutational pathways
Authors: Quinn Leonard M. , Haldenby Sam , Antzcak Philip , Fowler Anna , Bullock Katie , Kenny John , Gilbert Timothy , Andrews Timothy , Diaz-Nieto Rafael , Fenwick Stephen , Jones Robert , Costello-Goldring Eithne , Poston Graeme , Greenhalf William , Palmer Daniel , Malik Hassan , Goldring Chris 
Published: April 2023

AbstractPeri-hilar cholangiocarcinoma (pCCA) is chemorefractory and limited genomic analyses have been undertaken in Western idiopathic disease. We undertook comprehensive genomic analyses of a U.K. idiopathic pCCA cohort to characterize its mutational profile and identify new targets. Whole exome and targeted DNA sequencing was performed on forty-two resected pCCA tumors and normal bile ducts, with Gene Set Enrichment Analysis (GSEA) using one-tailed testing to generate false discovery rates (FDR). 60% of patients harbored one cancer-associated mutation, with two mutations in 20%. High frequency somatic mutations in genes not typically associated with cholangiocarcinoma included mTOR, ABL1 and NOTCH1. We identified non-synonymous mutation (p.Glu38del) in MAP3K9 in ten tumors, associated with increased peri-vascular invasion (Fisher’s exact, p < 0.018). Mutation-enriched pathways were primarily immunological, including innate Dectin-2 (FDR 0.001) and adaptive T-cell receptor pathways including PD-1 (FDR 0.007), CD4 phosphorylation (FDR 0.009) and ZAP70 translocation (FDR 0.009), with overlapping HLA genes. We observed cancer-associated mutations in over half of our patients. Many of these mutations are not typically associated with cholangiocarcinoma yet may increase eligibility for contemporary targeted trials. We also identified a targetable MAP3K9 mutation, in addition to oncogenic and immunological pathways hitherto not described in any cholangiocarcinoma subtype.
Optimized wake-superposition approach for multiturbine wind farms
Authors: Li Deshun , Chang Jixiang , Ma Gaosheng , Huo Chunyu , Li Rennian 
Published: April 2023

AbstractOptimizing the wind farm layout requires accurately quantifying the wind-turbine wake distribution to minimize interference between wakes. Thus, the accuracy of wind turbine wake superposition models is critical. The sum of squares (SS) model is currently touted as the most accurate, but its application in engineering is hampered by its overestimation of the velocity deficit of the mixed wake. Therefore, previous work relied on approximate power calculations for performing optimization. The physical meaning of the SS model is unclear, which makes optimization difficult. In this study, a univariate linear correction idea is proposed based on the linear increase phenomenon of the SS method error. The unknown coefficients are obtained by fitting experimental data. The results demonstrate that the proposed method can accurately quantify the full-wake two-dimensional distribution of the mixed wake.
Spatial antiferromagnetic spin texture as a nano-oscillator
Authors: Gerasimchuk Victor S. , Gorobets Yuri I. , Gorobets Oksana Yu. , Gerasimchuk Igor V. 
Published: April 2023

AbstractWe report a theoretical study of the localized spatial magnetization configuration, which is a confined spin configuration of the target skyrmion/hopfion type in an antiferromagnet with perpendicular magnetic anisotropy, and then we solve the particular problem of self-oscillations of such a topological spin texture. Using the energy approach, a self-consistent account of inhomogeneity of the characteristics of the topological magnetic spin texture was carried out. On this basis, the equation of free oscillations of the confined spin configuration magnetization was derived and its quasi-classical solution was found. For a thin ring spin texture, the frequency, period of oscillations and relative amplitude of the main tone of oscillations are found. For the first time, we determined the topological mass, inertial mass and total energy of the main tone of oscillations of such spatial spin texture. The self-oscillatory process of a spatial spin texture is interpreted as a magnetic nano-oscillator.
Association between bone marrow lesions and bone mineral density of the proximal tibia in end-stage osteoarthritic knees
Authors: Sasaki Eiji , Araki Ryo , Sasaki Tomoyuki , Wakai Yuji , Yamamoto Yuji , Ishibashi Yasuyuki 
Published: April 2023

AbstractThis retrospective cross-sectional study investigated the association between bone marrow lesions (BMLs) and bone mineral density (BMD) in the proximal tibia of end-stage osteoarthritic knees from a large patient sample. Overall, 1308 end-stage osteoarthritic knees were enrolled before total knee arthroplasty. The preoperative range of motion was recorded. Bone mineral density in the medial tibial plateau (MTP), lateral tibial plateau (LTP), and metaphysis were measured using dual-energy X-ray absorptiometry. The MTP/LTP, MTP/metaphysis, and LTP/metaphysis ratios were calculated. BMLs were scored using a whole-organ magnetic resonance imaging scoring system. The relationship between BMD and BML scores was investigated using linear regression analysis. The highest BMD was 0.787 ± 0.176 g/cm2at the MTP, followed by 0.676 ± 0.180 g/cm2and 0.572 ± 0.145 g/cm2at the metaphysis and LTP, respectively. The prevalence of BMLs was 90.4% and 24.2% in the MTP and LTP, respectively. In women, higher BML scores at the MTP were positively correlated with the BMD of the MTP (p < 0.001, r = 0.278), MTP/LTP (p < 0.001, r = 0.267), and MTP/metaphysis ratios (p < 0.001, r = 0.243). Regression analysis showed that higher BML scores in the MTP were correlated with higher BMD in the MTP (p < 0.001) and lower BMD in the LTP (p < 0.001). High BML scores in the MTP were positively associated with high BMD in the MTP, which also induced the medial to lateral imbalance of BMD in the proximal tibia.