bluuebunny/crossref_metadata_2025_split huggingface dataset:
Marker Nomenclature and Control of Type I and Type II Errors in Cattle Parentage Verification Using Microsatellite Markers
Authors: Modorov M. V. , Tkachenko I. V. , Kleshcheva A. A. , Sevost’yanov M. Yu. 
Published: January 2025

Abstract—
Wrong pedigree records reduce the quality of cattle breeding. For these reasons, DNA-based parentage verification has become an integral part of breeding work. Traditionally, in the Russian Federation, parentage verification was performed using immunogenetic markers. However, the improvement of technologies and strengthening of regulatory requirements have led to the fact that in recent years, microsatellite markers have replaced immunogenetics. Currently, there is no Russian protocol that clearly regulates the procedure of cattle parentage verification using microsatellite loci, which complicates the work of testing laboratories. In particular, the requirements for the number and nomenclature of genetic markers to be used in testing exist only for cattle, embryos, and sperm products transported between the member states of the Eurasian Economic Union. There are no requirements for the control of type I (false positive results) and type II (false negative results) errors, to be considered in certification. In this paper the approaches to solving these issues suggested by the International Society for Animal Genetics (ISAG), the International Committee for Animal Recording (ICAR), the Collegium of the Eurasian Economic Commission, as well as Russian regulatory documents governing forensic medical examination procedure related to the parentage assignment, will be considered. Based on the results of this review, a nomenclature of microsatellite markers and the examination protocol, which includes control of type I and type II errors, will be provided. Particular attention will be paid to the description of sources of type II errors and the need to control them in issuing a certificate.
Impact of resistance training on cardiometabolic health-related indices in patients with type 2 diabetes and overweight/obesity: a systematic review and meta-analysis of randomised controlled trials
Authors: Al-Mhanna Sameer Badri , Franklin Barry A , Jakicic John M , Stamatakis Emmanuel , Pescatello Linda S , Riebe Deborah , Thompson Walter R , Skinner James , Colberg Sheri R , Alkhamees Nouf H , Bin Sheeha Bodor , Gülü Mehmet , Alghannam Abdullah F , Batrakoulis Alexios 
Published: January 2025

ObjectiveTo evaluate the effects of resistance training on cardiometabolic health-related outcomes in patients with type 2 diabetes mellitus (T2DM) and overweight/obesity.DesignSystematic review and meta-analysis of randomised controlled trials (RCTs).Data sourcesPubMed, Web of Science, Scopus, Science Direct, Cochrane Library and Google Scholar databases were searched from inception up to May 2024. The search strategy included the following keywords: diabetes, resistance exercise and strength training.Eligibility criteria for selecting studiesRCTs published in English comparing resistance training alone with non-exercising standard treatment. Participants were adults diagnosed with T2DM and concurrent overweight/obesity (body mass index (BMI) ≥25 kg/m2).ResultsA total of 18 RCTs qualified involving 1180 patients (48.6/51.4 female/male ratio; 63.3±7.0 years; 29.3±4.3 kg/m2). Waist circumference (standardised mean differences (SMD) −0.85 cm, 95% CI −1.66 to −0.04), waist-to-hip ratio (SMD −0.72, 95% CI −1.30 to −0.15), high-density lipoprotein cholesterol (SMD +0.40 mg/dL, 95% CI 0.07 to −0.72), triglycerides (SMD −0.54 mg/dL, 95% CI −1.06 to −0.02), fasting blood glucose (SMD −0.65 mmol/L, 95% CI −1.19 to −0.12), fasting insulin (SMD −0.74 uIU/mL, 95% CI −1.12 to −0.36) and glycated haemoglobin (SMD −0.32%, 95% CI −0.63 to −0.01) improved compared with standard treatment. The risk of bias was low to unclear, and the quality of evidence was very low to moderate.ConclusionsResistance training as a standalone exercise intervention in the management and treatment of T2DM with concurrent overweight/obesity is associated with many cardiometabolic benefits when compared with standard treatment without exercise.PROSPERO registration numberCRD42022355612.
Developing a Shariah-compliant medical services framework in Malaysia: an expert system approach using fuzzy Delphi method and interpretive structural modelling
Authors: Che Jamaludin Farrah Ilyani , Abdullah Muhammad Ridhuan Tony Lim , Endut Mohd Nuri Al Amin , Saifuddeen Shaikh Mohd , Hamimi Kamarul Afendey , Harun Shariff 
Published: January 2025

IntroductionIn an increasingly diverse healthcare landscape, addressing the specific needs of Muslim patients has become paramount. The absence of comprehensive frameworks for Shariah-compliant healthcare services often poses challenges for healthcare professionals striving to provide care that aligns with Islamic values.ObjectiveThe objective of this study was to develop a comprehensive framework for Shariah-compliant healthcare services, ensuring alignment with Islamic practices in healthcare.Methods, setting, participantsThis consensus study employed a key input approach using the fuzzy Delphi method (FDM) and interpretive structural modelling. Conducted in Malaysia, the study involved 10 experts from various regions across the country. These experts were selected based on clear criteria that included professionals with experience in Islamic and/or healthcare, while those lacking relevant expertise were excluded.ResultsThe primary outcome was the identification of pertinent elements for the framework, with final elements measured based on expert consensus achieved through FDM. The panel of experts reached consensus on 10 essential elements that form the backbone of the framework for Shariah-compliant healthcare services. These elements include governance, medical ethics, patient care, human resources and professional development, facilities for Islamic worship (ibadah), spiritual care support, end-of-life care, Islamic environment, medicine and drugs, and affordability and accessibility.ConclusionUltimately, the development of this comprehensive framework is a crucial step in addressing the specific needs and concerns of Muslim patients worldwide. By incorporating the input and consensus of experts from various relevant fields, the resulting framework provides healthcare professionals with a solid foundation to deliver healthcare services that align with Islamic values, ultimately promoting the well-being of Muslim patients in Malaysia and tourists globally.
Equity in Basic Medical Education accreditation standards: a scoping review protocol
Authors: Shaheen Neelofar , Mahboob Usman , Sethi Ahsan , Irfan Muhammad 
Published: January 2025

IntroductionAccreditation in Health Professions Education is a pivotal process to ensure the quality of medical institutions and health professionals. ‘Equity’is an umbrella term reflecting the principles of justice and support to disadvantaged entities according to their needs. While commonly applied to various domains in medical education, there is potential to determine how ‘equity’is considered or discussed while practically implementing accreditation standards for Basic Medical Education (BME) globally.Methods and analysisThe scoping review protocol has been drafted in compliance with the Preferred Reporting Items for Systematic Reviews and Meta-Analyses-Protocol (PRISMA-P) checklist. We will conduct a planned scoping review according to the Joanna Briggs Institute (JBI) guidelines for scoping review and adhere to the PRISMA-Scoping Review extension (PRISMA-ScR) checklist. A systematic literature search, from inception to May 2024, will be conducted across search engines and databases including Google Scholar, PubMed, Web of Science, Wiley Online Library and ProQuest, in collaboration with a qualified librarian. Peer-reviewed research articles, commentaries, opinion papers and reviews that explicitly or implicitly discuss equity in reference to accreditation standards will be eligible for inclusion. A qualitative inductive thematic analysis will be employed to systematically identify categories and themes from the extracted data and draw conclusions.The review team anticipates the development of a conceptual map that will evolve during the synthesis of the literature, as a description of the term ‘equity’in the implementation of BME accreditation standards. We also anticipate finding any methodological or theoretical framework that is used in the literature to discuss the concept of ‘equity’in the implementation of BME accreditation standards.Ethics and disseminationThis scoping review aims to systematically map the literature on the burgeoning domain of accreditation in BME. The scoping review entails the collection and synthesis of literature and does not require ethical approval as per local regulations, as it does not involve primary data collection or interaction with human participants. The research findings will be disseminated through peer-reviewed publications, social media platforms, and academic presentations at workshops and conferences.
‘Do they care?’: a qualitative examination of patient perspectives on primary care clinician communication related to opioids in the USA
Authors: Olson Anthony W , Bucaloiu Andrei , Allen Clayton I , Tusing Lorraine D , Henzler-Buckingham Hillary A , Gregor Christina M , Freitag Laura A , Hooker Stephanie A , Rossom Rebecca C , Solberg Leif I , Wright Eric A , Haller Irina V , Romagnoli Katrina M 
Published: January 2025

ObjectivesThis substudy’s objectives were to (1) examine the transferability of a four archetype framework (simplified pattern of prototypical features) for patients at high risk for opioid use disorder (OUD) developed from a previous study with a similar population; (2) explore how patient preferences for terminology can inform clinician communication strategies for patients with OUD across archetypes and (3) explore how patient perceptions of opioid risks can inform clinician communication strategies across patient archetypes.DesignThis qualitative study collected data via semistructured phone interviews with patients about views on opioid-related discussions with primary care clinicians. Qualitative data were coded using the Rigorous and Accelerated Data Reduction technique and analysed via iterative inductive/deductive thematic analysis.Setting40 primary care clinics affiliated with two health systems (site1=Pennsylvania; site2=Minnesota, Wisconsin and North Dakota).Participants40 adults meeting one of the following: OUD diagnosis; taking medication for OUD (MOUD) and ≥3 opioid prescriptions in the previous year.ResultsThe aforementioned four archetype framework transferred well to the study sample and hinted at archetype differences in participant OUD-terminology preferences and opioid risk perceptions. Two additional archetypes of ‘in treatment/recovery for OUD and not taking MOUD’ and ‘in treatment/recovery for OUD and taking MOUD’ were identified. Participants best fitting archetypes 1–4 preferred clinicians to refrain from using addiction terminology to describe their relationship with opioids, finding the term ‘dependence’ as more appropriate and a signal that clinicians cared for patients. Participants who best first archetypes 5–6 felt ‘addiction’ was an appropriate, direct term that accurately described their condition, often using it themselves. Patients in all archetypes recognised risks of harm from using opioids, especially participants fitting archetypes 2, 5 and 6 who conveyed the greatest concern.ConclusionThe modified six archetype framework may help clinicians tailor their communication and care for patients diagnosed with or at high risk for OUD.Trial registration numberNCT04198428.
Impact of adverse childhood experiences on sensory thresholds in adults living with multimorbidity and chronic pain (the ACE-MAP study): protocol for an observational feasibility study
Authors: Senaratne Dhaneesha N S , Smith Blair H , Hales Timothy G , Marryat Louise , Colvin Lesley A 
Published: January 2025

IntroductionExposure to adverse childhood experiences (ACEs) is associated with a range of poor long-term health outcomes, including multimorbidity and chronic pain. Epidemiological evidence underpins much of this relationship; however, psychophysical testing methods, such as quantitative sensory testing (QST), may provide valuable insights into potential mechanisms. Previous studies have shown inconsistent links between ACEs and QST, but the QST profiles of people with multimorbidity have not been reported. We hypothesise that exposure to ACEs is associated with lowered QST thresholds (ie, experience of pain with milder stimuli) and that this association is stronger in adults with multimorbidity and/or chronic pain. The ACE-MAP study is a cross-sectional feasibility study with the primary aim of assessing the feasibility and acceptability of the proposed study procedures. The secondary aim is to generate preliminary data to understand the impact of ACEs on QST thresholds.Methods and analysisWe plan to recruit 40 participants, with 10 in each of the following groups: (1) chronic pain with multimorbidity; (2) chronic pain without multimorbidity; (3) multimorbidity without chronic pain; and (4) controls. Participants will complete a series of questionnaires (including on ACEs, chronic pain and long-term conditions) and will then take part in QST assessments. The primary study outcomes will include measures of feasibility and acceptability of the proposed study design. The secondary study outcomes will include exploratory analysis on the relationship between ACEs and QST thresholds.Ethics and disseminationThe study was approved by the Scotland B Research Ethics Committee (reference: 24/SS/0031). Results from the study will be presented at scientific conferences, published in a peer-reviewed journal and shared with patients and members of the public through other media streams.Trial registration numberISRCTN10049430.
Relationship between staff experience and patient outcomes in hospital settings: an overview of reviews
Authors: Bragge Peter , Delafosse Veronica , Kellner Paul , Cong-Lem Ngo , Tsering Diki , Giummarra Melita J , Lannin Natasha A , Andrew Nadine , Reeder Sandra 
Published: January 2025

ObjectivesThis review aimed to investigate the relationship between staff experience and patient health and experience outcomes in hospital inpatient settings.DesignSystematic review of reviews.MethodsSearches were performed in Medline (OVID), CINAHL and Google Scholar using key terms from relevant review articles. The search was conducted on 28 August 2023. Inclusion criteria were systematic or narrative reviews in English from 2020 onwards, focusing on inpatients in hospital and related tertiary care facilities, examining the relationship between staff experience and patient outcomes. A review of reviews approach was used, with broad definitions for staff experience (eg, hospital culture, stress and burnout) and patient outcomes (eg, adverse events and patient experience). Independent screening and quality appraisal were conducted by two researchers. An evidence map of links between staff experience and patient outcomes was created. The methodological quality of systematic reviews was assessed using the AMSTAR 2 tool and narrative reviews with the SANRA tool.ResultsFrom 2365 citations, 21 reviews (18 systematic, 3 narrative) were included. Review quality ranged from moderate to high. Mapping revealed 66 associations between staff experience and patient outcomes. Common associations included burnout, stress and fatigue with adverse events (six reviews); communication with patient satisfaction (four reviews) and teamwork with patient satisfaction (four reviews).ConclusionsStaff burnout, teamwork and communication practices directly impact adverse events and patient satisfaction. These findings guide hospital managers and clinicians in improving health service policies and practices. Further research is needed to strengthen the evidence base.
Relationship between vaginal and gut microbiome and pregnancy outcomes in eastern Ethiopia: a protocol for a longitudinal maternal-infant cohort study (the EthiOMICS study)
Authors: Weldegebreal Fitsum , Ayana Desalegn Admassu , Wilfong Tara , Dheresa Merga , Yadeta Tesfaye Assebe , Negesa Akewok Sime , Demmu Yohannes Mulugeta , Tesfa Tewodros , Alemu Tegbaru Nibrat , Eticha Tadesse Gure , Geremew Abraham , Roba Kedir Teji , Abdissa Alemseged , Assefa Nega , Negash Abel Abera , Cools Piet , Tura Abera Kenay 
Published: January 2025

IntroductionAlthough evidence exists on the impact of microbiota on pregnancy outcomes in many high-resource settings, there is a lack of research in many low-resource settings like Ethiopia. This study aims to fill this gap by studying the gut and vaginal microbiota changes throughout pregnancy and assess how these changes relate to pregnancy outcomes among a cohort of pregnant women in eastern Ethiopia.Methods and analysisVaginal and stool samples will be collected using DNA/RNA Shield Collection kits three times starting at 12–22 weeks, 28–36 weeks and at birth (within 7 days). Postnatally, newborns’ skin swabs (at birth) and rectal swabs will be obtained until 2 years of age. Moreover, breast milk samples at birth and 6 months and environmental samples (water, indoor air and soil) will be collected at enrolment, birth, 6, 12 and 24 months post partum. DNA will be extracted using Roche kits. Metagenomic sequencing will be performed to identify metataxonomic profiling and assess variations in microbial profiles, and α and β diversity of the microbiota. Information on socioeconomic, behavioural, household and biological factors will be collected at enrolment. The collected data will be coded, entered into EpiData 3.1 and analysed using Stata 17.Ethics and disseminationThe Institutional Health Research Ethics Review Committee (Ref No. IHRERC/033/2022) of Haramaya University, Ethiopia has approved this study ethically. Written informed consent regarding the study and sample storage for biobanking will be obtained from all participants. Results will be published in international peer-reviewed journals, and summaries will be provided to the study funders. Clinical study data will be submitted to Data Compass (https://datacompass.lshtm.ac.uk/), and molecular profiles of the microbiome and whole-genome sequences will be submitted to the European Nucleotide Archive (https://www. ebi.ac.uk/ena). Requests for data should be directed to daberaf@gmail.com. The decision to share data will be made by the study steering committee under the College of Health and Medical Sciences, Haramaya University, Ethiopia.
Sensory alterations and immunological changes during the chronification of postsurgical pain: a study protocol for a prospective observational cohort study
Authors: van Driel Marjelle E C , van Veenendaal Nadine , Vernooij Lisette Margaretha , Eijkelkamp Niels , Koenderman Leo , Timmerman L , Custers Roel J H , Delawi Diyar , Huygen Frank J P M , Rijsdijk M 
Published: January 2025

IntroductionChronic postsurgical pain (CPSP) represents a widely underdiagnosed and often poorly treated medical problem, affecting 10–50% of all surgical patients, exhibiting neuropathic features in 35–60%. It is hypothesised that surgery-induced tissue damage and the subsequent immune response cause sensory alterations in the early postoperative period, ultimately leading to a chronic neuropathic or nociplastic pain state. The ‘Sensory Changes and Immunological parameters in Postsurgical pain’ study (SCIP-Pain study) was designed to test this hypothesis and identify sensory alterations and changes in the immunological response that are related to the development of CPSP with neuropathic features.Methods and analysisThis protocol describes the SCIP-Pain study—an ongoing prospective observational cohort study involving 150 adult patients undergoing elective lower extremity orthopaedic surgery. Study participants complete questionnaires, undergo quantitative sensory testing (QST) and provide blood samples to assess the immunological response at various time points: before surgery, 2 weeks and 3 months after surgery. To reduce dimensionality, cluster analyses will be conducted on QST and immunological parameters. Cluster allocation, along with other preselected candidate predictors, will subsequently be used in a generalised mixed-effects model to predict CPSP with neuropathic features within 3 months after surgery as the primary outcome.Ethics and disseminationThis study received approval from the Medical Ethics Committee NedMec (protocol NL77085.041.21), as well as from all participating centres. The study results are expected to be published in peer-reviewed journals and disseminated at international conferences.
Clinical and ultrasound assessment of body composition in preterm infants at discharge: an observational study
Authors: Izquierdo Renau Montserrat , García González Elsa , Balcells-Esponera Carla , del Rey Hurtado de Mendoza Beatriz , Inarejos Clemente Emilio J , Iglesias-Platas Isabel 
Published: January 2025

ObjectivesWhile the target of growth of very preterm infants (VPIs) during Neonatal Intensive care unit (NICU) admission is still controversial, the most accepted objective is that they should follow their intrauterine trajectory in terms of growth and body composition (BC). BC is difficult to measure in clinical daily routine but proxies like body ratios and skinfolds have been used. Prenatal and postnatal factors can influence the growth and BC of VPIs in the NICU.Design, setting and patientsWe conducted a case-control study, including preterm infants born before 32 weeks gestational age at term-corrected age (TCA) and healthy late preterm or term infants as controls and also a retrospective cohort analysis of factors influencing VPI’s BC at discharge. Patients had an anthropometric evaluation at discharge including weight, length, head circumference, body circumferences (waist, arm), skinfolds and abdominal ultrasound (US).Results191 VPIs were eligible for discharge visits, but only 83 had a complete evaluation and we collected 26 controls. VPIs at TCA were smaller in weight, length and head circumference but had greater ratios (arm fat-to-circumference and waist-to-length). Abdominal fat assessed by US was smaller in VPIs compared with term infants. Intrauterine growth restriction-VPI remained smaller at TCA but experienced less weight z-score loss. Sex did not seem to influence growth and BC proxies at TCA. Higher nutritional support during the first 2 weeks of life was related to a lower loss of length z-scores, and exclusive human milk feeding correlates with arm fat-to-circumference ratio.ConclusionsGrowth and BC of VPIs can be approached using simpler measures in clinical practice. Arm skinfolds and arm circumferences point to a greater adiposity of VPIs at TCA compared with term infants, while US does not show a greater visceral adiposity. Nutritional factors played a small effect in BC at the time of discharge.