bluuebunny/crossref_metadata_2025_split huggingface dataset:
Mapping high probability area for the Bacillus anthracis occurrence in wildlife protected area, South Omo, Ethiopia
Authors: Joka Fekede Regassa 
Published: June 2023

Abstract

Anthrax is a zoonotic disease caused by a spore-forming gram-positive bacterium, Bacillus anthracis (BA), in which soil is the primary reservoir. The geographic distribution of the disease appears to be restricted by a combination of climatic and environmental conditions. Among the top five zoonotic diseases, Anthrax is the second priority zoonosis in Ethiopia. Increased anthropogenic factors inside wildlife protected areas may worsen the spillover of the disease from domestic animals to wildlife. Consequently, the prediction of the environmental suitability of BA spores to locate a high-risk area is urgent. Here we identified a potentially suitable habitat for BA spores survival and a high-risk area for appropriate control measures. Our result revealed that a relatively largest segment of Omo National Park located on the western side and more than half of the total area of Mago National Park bordering Hamer, Bena Tsamay, and south Ari were categorized under a high-risk area for the anthrax occurrence in the current situation. Therefore, the findings of this study provide the priority area to focus and allocating resources for effective surveillance, prevention, and control of anthrax before it cause devastating effect on wildlife.
Association study of 3-UTR haplotype of HLA-G gene with Lupus
Authors: Chyad Ihsan Alwan , Haghi Mehdi , Feizi Mohammad Ali Hosseinpour , Kondori Mohammad Khalaj , Ardalan Mohammad Reza 
Published: June 2023

Abstract
Background
Human leukocyte antigen-G (HLA-G) is a protein that plays a critical role in immune regulation and tolerance. Systemic lupus erythematosus (SLE) is a complex autoimmune disease that results from a combination of genetic and environmental factors. Studies have shown that HLA-G polymorphisms and mutations may contribute to the development of SLE.
Objective
The aim of this study was to investigate the association between polymorphisms in the 3'-UTR region of the HLA-G gene and SLE.
Methods
DNA was extracted from 100 SLE patients and 100 control samples, and PCR was used to amplify the target sequence. The allele and genotype frequencies were calculated, and haplotypes were evaluated using Haploview v.4.2 software, with linkage disequilibrium calculated.
Results
The results showed that the + 2960 Ins allele was significantly associated with SLE as a risk factor, while the Del allele was protective. The + 3010 C allele and + 3187 A allele were also significantly associated with SLE at both the allele and genotype level. The + 3142 GG homozygote was significantly associated with SLE at the genotype level. Haplotype block analysis found that the UTR-2 haplotypes were significantly associated with SLE as a risk factor, while the UTR-1 haplotype was protective. These findings provide valuable insights into the genetic factors contributing to the risk of developing SLE.
Conclusion
The study highlights the significance of polymorphisms in the 3'-UTR region of the HLA-G gene in SLE susceptibility and suggests that these variants may have potential as diagnostic or therapeutic targets.
A General Room Temperature Suzuki−Miyaura Polymerization Process for Organic Electronics
Authors: Xiong Haigen , Lin Qijie , Lu Yu , Zheng Ding , Li Yawen , Wang Song , Xie Wenbin , Li Congqi , Zhang Xin , Lin Yuze , Wang Zhi-Xiang , Shi Qinqin , Huang Hui , Marks Tobin 
Published: June 2023

Abstract
π-conjugated polymers (CPs) have broad applications in high-performance optoelectronics, energy storage, sensors, and biomedicine. However, developing green and efficient methods to precisely synthesize well-defined CP structures on a large scale remains challenging and critical for their industrialization. Here, a room-temperature, scalable, homogeneous Suzuki − Miyaura-type polymerization methodology is reported with broad generality validated for 24 CPs including donor − donor, donor − acceptor, and acceptor − acceptor connectivity classes, and consistently yielding device-quality polymers with molecular masses greater than 100.0 kDa. Furthermore, the present polymerization protocol significantly reduces homocoupling structural defects, yielding more structurally regular and higher performance electronic materials and opto-electronic devices than in conventional thermally-activated polymerizations. Experimental and theoretical studies reveal that a borate-transmetalation process plays a key role in suppressing protodeboronation, which is critical for large scale structural regularity. Thus, these results provide a general polymerization tool for mass producing device-quality CPs with well-defined structural regularity.
Management of peritoneal carcinomatosis from appendiceal mucinous adenocarcinoma by cytoreductive surgery plus hyperthermic intraperitoneal chemotherapy: Experience on 48 patients from a single center
Authors: Jiao Jie , Li Chengzhen , Yu Guanying , Liu Zhenpeng , Zhang Lei , Shi Xiaoyan , Yan Jingdu , Zhang Houjun , Shi Jingbo , Guo Peiming 
Published: June 2023

Abstract
Purpose To evaluate the efficacy and safety of cytoreductive surgery (CRS) combined with hyperthermic intraperitoneal chemotherapy (HIPEC) in the treatment of patients with peritoneal carcinomatosis (PC) from appendiceal mucinous adenocarcinoma.Methods The clinical data of 48 cases of PC with mucinous appendiceal adenocarcinoma treated with CRS + HIPEC were retrospectively analyzed. The study indices included survival, perioperative safety, and adverse reactions.Results The peritoneal cancer index (PCI) score ranged from 1 to 39 in the 48 patients with peritoneal cancer of appendix adenocarcinoma, with a median score of 21. The degree of tumor reduction (CC) was 0 to 3, with a median score of 2. The follow-up period ranged from 16 to 44 months. Of the 48 patients, 18 patients (37.5%) died and 30 patients (62.5%) survived. The median survival time was 37 months (95% CI: 26.0-47.9 months). The 1-, 2-, and 3-year survival rates were 78.0%, 60.9%, and 51.4%. The 1-, 2-, and 3-year survival rates in patients with PCI ≥ 20 were 63.6%, 43.8%, and 32.8%, respectively, while the patients with PCI < 20 had rates of 95%, 81.8%, and 72.7% (P = 0.0139). The 1-, 2-, and 3-year survival rates were 64.4%, 40.9%, and 40.9% in patients with CC > 1, and 95%, 88.2%, and 69.4% in patients with CC ≤ 1 (P = 0.004). The incidence of perioperative adverse events was 8.3%, including 3 cases of incision infection and 1 case of intestinal anastomotic fistula.Conclusion CRS + HIPEC is feasible and effective in the treatment of patients with peritoneal carcinoma from appendiceal mucinous adenocarcinoma.
Comparative outcome of short proximal femoral nail versus proximal femoral locking compression plate in the management of unstable trochanteric fractures
Authors: DAS ABHIJIT , G HARIKRISHNAN N , NAMBIAR DEVAN P , CHAKRABORTY INDRAJIT , JHA SOURABH KUMAR , SHIRDINAYAK TIRUPATI SHIVANAND , MONDAL ANINDYA SANKAR 
Published: June 2023

Abstract
Introduction: Trochanteric fractures are mostly due to RTA and falls. Unstable trochanteric fractures include those with a reverse oblique fracture line, intertrochanteric comminution, big posteromedial fragment, subtrochanteric extension, a broken greater trochanter, and lateral cortex breach. As per the AO Classification of intertrochanteric fractures, AO31-2.2, AO31-2.3, AO31-3.1, AO31-3.2, and AO31-3.3 fall under the category of unstable trochanteric fractures. Internal fixation is mandatory to provide early mobilization of patients with partial weight-bearing and prevent further complications.Materials and Methods 43 patients more than 18 years old with unstable trochanteric fractures, less than 3 weeks old trauma were included in the prospective study for 1 year period (1st June 2020 to 31st May 2021). 22 cases were treated with short proximal femoral nail (PFN) and 21 cases were treated with proximal femoral locking compression plate (PFLCP).Results (P < 0.05), mean duration of surgery, blood loss was less in the PFN group (67minutes; 80mL) than PFLCP group (99minutes; 152mL). Union and partial weight-bearing was seen earlier in PFN group (14.1weeks; 10.6weeks) than in PFLCP group (18.7 weeks; 15.8weeks) (P < 0.05). Good-excellent outcome was seen in 100% cases in PFN group and 85.71% cases in PFLCP group. There were 3 cases of delayed union in PFLCP group and 1 in PFN group. 1 PFLCP case had malunion in varus deformity.Conclusion Short PFN is the optimum implant in treatment of unstable trochanteric fractures as it is an intramedullary, load sharing device with short lever arm and hastens biological healing with early ambulation and minimal complications.
Towards a Valid Measure of the Economic Value of Museum Experiences: An example from Finland
Authors: Falk John H. , Claudio Nicole , Myllykoski Mikko , Seppälä Sauli , Sivonen Pauli , Tamminen Jonna 
Published: June 2023

Abstract

This article describes an effort to calculate the public and economic value of museum experiences. A significant feature of the research was its use of outcome metrics grounded in decades of visitor research on the perceived value of visiting a museum. The article lays out the underlying theoretical approach and presents data from a year-long study of visitors to eight Finnish museums. Results showed that museum users perceived that their visit experience resulted in enhanced, durable feelings of personal, intellectual, social and physical well-being, with valuations of the economic benefits of these enhanced feelings of well-being equivalent to €864/visit. As was the case of the eight museums in the study, most museums annually serve tens to hundreds of thousands of visitors, thus annually individual museums create on the order of hundreds of millions of Euros of value for their communities and collectively provide value in the billions of Euros. The findings from this research make it possible for museums to make a more valid and credible case for the significant value they create and for the vital role they play in supporting the public’s well-being.
Ultra-High Photosensitivity of Nb2O5/Si Prepared by DC reactive Magnetron Sputtering Technique
Authors: Hathal Yahya , Ibrahim Isam , Khalaf Mohammed 
Published: June 2023

Abstract
The aim of this work is to prepare Nb2O5 thin films utilizing DC reactive magnetron sputtering in a range of sputtering powers (25, 50, and 75 W) on silicon wafers and quartz substrates. The structure, morphological, optical, and electrical features of the manufactured Nb2O5 thin films were studied with the assistance of an XRD analysis, FE-SEM, ultraviolet-visible spectrophotometer, and I-V tests. According to the X-ray diffraction, Nb2O5 is a natural polycrystalline substance with a hexagonal crystal structure. In addition, the images obtained from the FE-SEM revealed that the films are uniformly deposited and that the configuration and size of the nanostructures highly depend on the deposition parameters. On the other hand, from the EDS results, the Nb content increased with increasing DC sputtering power. Furthermore, these thin films were exceptionally responsive to the ultraviolet light with a wavelength of 350 nm, because the optical energy gap in the ultraviolet region was in the middle of 3.73 and 4.47 eV, with a maximum photosensitivity of 514.89% for films prepared at a power of 50 W. Finally, the thin films made of Nb2O5 are well-suited for use in visible-blind ultraviolet (UV) sensors and circuits made of optoelectronics, specifically the ones that work in the area of UV-A region.
Effect of transcranial direct current stimulation on FRN and P2 during the performance of an auditory monetary incentive delay task
Authors: Grigoreva Anastasia , Gorin Aleksei , Klyuchnikov Valery , Dutov Ivan , Moiseeva Victoria , Klucharev Vasily , Shestakova Anna 
Published: June 2023

Abstract
Transcranial electrical stimulation (tES) is a powerful tool for elucidating the causal relationship between specific brain regions and behaviour. While tES generates consistent results in sensorimotor research, cognitive studies present a more diverse, often ambiguous response to stimulation. To further understand these complexities, our study employed cathodal transcranial direct current stimulation (tDCS) to modulate medial–frontal cortex activity during an auditory monetary incentive delay (MID) task. Participants responded to acoustic cues signaling expected monetary losses. We analysed feedback-related negativity (FRN), reflecting a reward prediction error processing when participants encountered losses and compared it to no-loss conditions across two subsequent MID task sessions. We also assessed the exogenous P2 response to stimulus onset, independent of monetary contingencies, anticipating an inhibitory effect of cathodal tDCS on both FRN and P2. Contrarily, we observed a facilitatory effect of stimulation on FRN and a suppressed learning effect of P2. The sham group demonstrated a difference in P2 amplitude between the first and second session, which was absent in the cathodal tDCS group. Our findings regarding the multidirectionality of tDCS effects on cognitive function challenge the utility of tES as a readily employable method for testing brain causality in highly complex neurocognitive events, such as decision-making.
Bacterial and fungal load quantitative and qualitative assessment of indoor air in Portuguese long-term and acute healthcare settings
Authors: Santos-Marques Catarina , Teixeira Camila , Pinheiro Rafael , Brück Wolfram Manuel , Pereira Sónia Gonçalves 
Published: June 2023

Abstract
Background
In healthcare-associated infections, the major transmission route is assumed to be the direct or indirect contact between patients, staff, and the environment. However, little is known about the air role as a pool for pathogens and multidrug resistance (MDR) in healthcare settings. The aim of this study was to quantitatively and qualitatively compare microbial air quality in a Portuguese long-term healthcare unit (LTHU) and a central hospital (CH).
Methods
During 2021 and 2022, air samples were collected fortnightly in both settings and their microbial load (bacteria and fungi) was determined (colony forming units – CFU/m3). Sampled sites were classified according to area, room capacity and occupation, activity and related infection potential risk. Bacterial isolates were randomly selected for identification by Matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometry, antimicrobial susceptibility testing and polymerase chain reaction (PCR) screening of extended-spectrum beta-lactamases and metallo-beta-lactamases genes.
Results
A total of 192 air samples were collected, 86 from LTHU and 106 from CH. Statistically significant differences in mean bacterial load in LTHU (367 CFU/m3) and CH (230 CFU/m3) was observed, but not in mean fungal load (LTHU: 87 CFU/m3; CH: 117 CFU/m3). CH bacteria and fungi loads were statistically significantly higher in sites with outpatients or no patients compared to those with inpatients as well as in support services compared to curative care areas. No statistically significant differences were found regarding these variables in LTHU.
Prebiotic effects of commercial apple juice in high-fat diet fed rat
Authors: Kon Risako , Ikarashi Nobutomo , Ohkuma Mayumi , Toyonaga Misato , Tomimoto Rei , Sakai Hiroyasu , Hosoe Tomoo , Kamei Junzo 
Published: June 2023

Abstract
Apples are one of the most frequently consumed fruits and are effective in preventing lifestyle-related and other diseases. However, some people may avoid consuming apples because of the time required to peel them. Processed apple products such as juice are easy to consume, but few studies have been conducted to evaluate their health benefits. In this study, we analyzed the health benefits of consuming commercially available apple juice, focusing on changes in the gut microbiota, which plays an important role in maintaining human health. Rats were fed apple juice ad libitum, and the relative abundances of various gut microbes in fecal samples were analyzed. In addition, rats fed apple juice were treated with a high-fat diet, and body weight, plasma triglyceride, glucose, and cholesterol levels were measured. The relative abundance of Clostridium cluster XIVa did not change with the treatment of apple juice, but the relative abundance of Clostridium cluster IV was significantly decreased. In contrast, the relative abundances of Lactobacillus and Bifidobacterium, which provide benefits to the human body, were significantly increased by 3-fold and 10-fold, respectively, with apple juice consumption. When apple juice-treated rats were fed a high-fat diet, the increase in body weight, liver fat, and blood lipid parameters were all suppressed compared to high-fat alone group. This study suggests that the consumption of commercial apple juice changes the gut microbiota, exerts a prebiotic effect, and is effective in improving lifestyle-related diseases.
An Exploration of Online Technoliteracy Capability Teaching and Learning in Early Years Classrooms
Authors: Falloon Garry 
Published: June 2023

Abstract
The increasing use of digital devices by young children, has led to calls for earlier teaching for information literacy. However, some research indicates reluctance to do this, due to perceived limitations of young children and notions about what is and is not ‘appropriate’ for them learn. This study examines this proposition, through analysis of 6 and 7 year olds’ application of ‘Technoliteracy’ capabilities during a unit of learning about Matariki (the Maori new year). It used an updated and expanded revision of Durrant and Green’s L(IT)eracy capability model, to understand how the students applied ‘Technoliteracy’ capabilities to online research and production of an information artefact for an identified audience. Although results were mixed, data was found of students’ productive engagement of ‘Technoliteracy’ capabilities aligned with Durrant and Green’s dimensions, suggesting that with developmentally-appropriate curriculum and pedagogy they were capable of integrating these for meaning making, judging meaning quality, and meaning sharing and communication. Given increasingly ubiquitous access to devices from a young age, results indicate that serious consideration should be given to teaching basic ‘Technoliteracy’ capabilities in early years classrooms.
Seasonal variations of water physico-chemistry, heavy metal concentrations and ecological abundance and biodiversity of fish from two Lagoon streams
Authors: Jolaosho Toheeb Lekan , Olayeri Abraham , Oladipupo Ibrahim Olawale , Saba Abbas Olayiwola , Mustapha Adejuwon , Alonge Faith 
Published: June 2023

Abstract
Background
The fluctuation trends of ecological parameters and the biodiversity of aquatic living resources in freshwater environments are influenced majorly by anthropogenic activities. However, little or no study has been reported on the influence of natural events particularly seasons, on water physico-chemistry, heavy metal concentrations and their impacts on the ecological assemblages of fish in interlinked lagoon systems. The present study was carried out to estimate the influence of seasonal variations on the physico-chemical parameters, heavy metal concentration of water and biodiversity, and richness of fish from two lagoons with a view to ascertaining the significance of seasons on aquatic ecosystems and their resources.
Methods
The sampling lasted 12 months (from December 2021 to November 2022), during which both dry and rainy seasons were experienced. Samples of water and fish were collected twice per month. Water quality analysis was done using BOYD titration and APHA standard methods. The heavy metal contents were determined using the atomic absorption spectrophotometer. Diversity indices were computed using Microsoft Excel 2015.
Results
There were inconsistencies in the results obtained, and as a result, statistical differences (P < 0.05) were observed. Most of the water parameters were at their highest during the dry season. High heavy metal concentrations, particularly Cd, Pb, Zn, and Fe at (P < 0.05), were recorded during the dry season. A total of 920 and 953 individual fish from 22 and 26 families were obtained from Lekki Lagoon during the dry and wet seasons, respectively. In the Lagos Lagoon, a total of 1299 and 1772 individual fish, which belong to 24 and 25 families, were collected during the dry and rainy seasons, respectively. The best diversity indices, such as Shannon Weiner, Simpson's dominant index, Margalef, Menhinick, and the evenness index, were obtained during the rainy season.
Conclusion
Our results suggested that variation in seasons strongly influenced water quality parameters and heavy metal concentrations, as well as fish species biodiversity in the study areas. Hence, seasonal patterns in relation to aquatic ecosystems should be taken into account when making long-term policies for the sustainability of fisheries resources.
Impact of Tender Points, Average Pain, Catastrophizing, Central Sensitization, Anxiety, and Depression on Quality of Life in Individuals with Sickle Cell Disease.
Authors: de Oliveira Lilian Anabel Becerra , Lopes Tiago da Silva , de Sousa Juliane Nascimento , Silva Edvan Santos da , Ribeiro Sânzia Bezerra , Baptista Abrahão Fontes , Sá Katia Nunes 
Published: June 2023

Abstract

Purpose
To analyze the impact of tender points in the body, average pain, Catastrophizing, Central Sensitization (CS), Anxiety, and Depression on the QoL of adults with SCD.
Method
This is a descriptive cross-sectional study. It collected sociodemographic data and applied the Brazilian-Portuguese Central Sensitization Inventory, the Brief Pain Inventory, the Hospital Anxiety/Depression Scale, the Brazilian-Portuguese Pain Catastrophizing Scale, and the Short Form Health Survey 36. Statistical analysis was done to define associations (Chi-square or Fisher's Exact) and correlation (Pearson correlation test). The alpha level was 5%, and the Beta of 80%.
Results
100 individuals participate, with genotypes HbSS/HbSC. 69% were women. Age 34.14 + 10.12 years. Pain intensity 4.20 + 2.67; 71% had chronic pain; 60% had widespread pain; 59% had CS; 33% had Anxiety; and 18% had Depression. The lowest mean score for QoL was for the Physical Aspect domain (35.55 + 40.16). Anxiety, Average Pain, CS, and Catastrophizing correlated with all the domains of QoL. Anxiety and CS showed a significant negative influence over the Mental Health domain (F(2.95) = 43.014 p < .001; adjusted R2 = .464), explaining 46.6% of the outcome. Catastrophizing and CS had a significant negative influence on the General Health Status (F(2.95) = 21.592 p < .001; adjusted R2 = .298), explaining 29.8% of the outcome; and Anxiety, Pain in lower limbs, and Depression had a significant negative influence on Pain domain (F(2.95) = 15.207 p < .001; adjusted R2 = .270), explaining 27% of the outcome.
Conclusion
Anxiety, Depression, Catastrophizing, and CS are significant factors that impact QoL. In a population of SCD individuals, the primary objective is to control pain, which leads to chronic pain directly affecting these predictors and, inevitably, QoL.