bluuebunny/crossref_metadata_2025_split huggingface dataset:
Novel tricyclic pyrrolo-quinolines as pharmacological correctors of the mutant CFTR chloride channel
Authors: Renda Mario , Barreca Marilia , Borrelli Anna , Spanò Virginia , Montalbano Alessandra , Raimondi Maria Valeria , Bivacqua Roberta , Musante Ilaria , Scudieri Paolo , Guidone Daniela , Buccirossi Martina , Genovese Michele , Venturini Arianna , Bandiera Tiziano , Barraja Paola , Galietta Luis J. V. 
Published: May 2023

AbstractF508del, the most frequent mutation in cystic fibrosis (CF), impairs the stability and folding of the CFTR chloride channel, thus resulting in intracellular retention and CFTR degradation. The F508del defect can be targeted with pharmacological correctors, such as VX-809 and VX-445, that stabilize CFTR and improve its trafficking to plasma membrane. Using a functional test to evaluate a panel of chemical compounds, we have identified tricyclic pyrrolo-quinolines as novel F508del correctors with high efficacy on primary airway epithelial cells from CF patients. The most effective compound, PP028, showed synergy when combined with VX-809 and VX-661 but not with VX-445. By testing the ability of correctors to stabilize CFTR fragments of different length, we found that VX-809 is effective on the amino-terminal portion of the protein that includes the first membrane-spanning domain (amino acids 1–387). Instead, PP028 and VX-445 only show a stabilizing effect when the second membrane-spanning domain is included (amino acids 1–1181). Our results indicate that tricyclic pyrrolo-quinolines are a novel class of CFTR correctors that, similarly to VX-445, interact with CFTR at a site different from that of VX-809. Tricyclic pirrolo-quinolines may represent novel CFTR correctors suitable for combinatorial pharmacological treatments to treat the basic defect in CF.
Antimicrobial properties of promising Zn–Fe based layered double hydroxides for the disinfection of real dairy wastewater effluents
Authors: Abdel Aziz Sahar Abdel Aleem , GadelHak Yasser , Mohamed Manar Bahaa El Din , Mahmoud Rehab 
Published: May 2023

AbstractBacterial resistance to conventional antibiotics is a serious challenge that requires novel antibacterial agents. Moreover, wastewater from dairy farms might contain countless number of pathogens, organic contaminants and heavy metals that consider a threat to the terrestrial and aquatic environment. Therefore, the development of cost-effective, highly operation-convenient, recyclable multifunctional antimicrobial agents became an urgent necessity. Layered double hydroxides (LDH) have shown promising results as antibacterial agents. However, more work is required to further investigate and improve the antimicrobial performance of LDH structures against pathogens. In this study three Zn–Fe based LDH were investigated for real dairy wastewater disinfection. The three LDH samples were cobalt substituted Zn–Fe LDH (CoZnFe), magnesium substituted Zn–Fe LDH (MgZnFe) and MgZnFe-Triazol LDH (MgZnFe-Tz) nanocomposite. Seventy-five wastewater samples were collected from a dairy farm sewage system. The sensitivity of isolated pathogens was tested against two commonly used disinfectants (Terminator and TH4) and was assessed against the three LDH samples at different concentrations. The overall prevalence of S.agalactiae, S.dysgalactiaeandStaph. aureuswas significantly at 80.0% (P-value = 0.008, X2 = 9.700). There was variable degree of resistance to the tested disinfectants, whereas the antimicrobial activity of CoZnFe LDH was increased significantly at a concentration of 0.005 mg/L followed by MgZnFe LDH while MgZnFe-Tz LDH showed minor antibacterial potency. It was concluded that CoZnFe LDH showed a better biocidal activity in killing the isolated resistant pathogens, making it a good choice tool in combating the zoonotic microbes in wastewater sources.
BIM-based digital platform and risk management system for mountain tunnel construction
Authors: Liu Naifei , Guo Desai , Song Zhanping , Zhong Shiming , Hu Ruoqi 
Published: May 2023

AbstractDuring the construction of mountain tunnels, there are often various intricate and mutable potential hazards, the management and control of which are crucial to ensuring the safety of such construction. With the rapid advancement of engineering information technologies, including Building Information Model (BIM), the internet, big data, and cloud computing, dynamic management of mountain tunnel construction will inevitably become a prevailing trend. This paper proposes a new digital approach to realize the informatization and visualization of risk management in mountain tunnel construction, by combining monitoring measurement with advanced geological prediction based on BIM technology. The proposed approach suggests a BIM-based digital platform architecture for mountain tunnel construction, which is comprised of five layers—basic, model, data, application, and user. The integration of these five layers can realize risk management information during the construction of mountain tunnels. In addition, a set of dynamic risk management systems, including risk monitoring, identification, and assessment, can be established based on the digital platform. The digital platform and dynamic risk management system proposed in this paper have certain advantages in the construction of mountain tunnels, providing a new and significant way for the management of safety risks in such construction projects.
RNU12 inhibits gastric cancer progression via sponging miR-575 and targeting BLID
Authors: Wang Shaoli , Zou Changyan , Lin Xinyi , Hu Dan , Su Ying , He Huocong , Zheng Xiongwei , Zhang Lurong , Huang Tao , Liao Jin-rong , Lin Xiandong 
Published: May 2023

AbstractGastric cancer (GC) is one of the major causes of cancer deaths with 5-year survival ratio of 20%. RNU12 is one of long noncoding RNAs (lncRNAs) regulating the tumor progression. However, how RNU12 affecting GC is not clear. qRT-PCR was utilized for determining the RNU12 expression in cell lines, 113 cases of paired gastric cancer (GC) and their adjacent normal gastric tissues. The biofunction alterations of RNU12 were assessed by its overexpression or knockdown in GC cells. MTT and cloning assay were assayed for the cell proliferation, the flow cytometry for the detection of cell cycle and the wound healing assay (WHA) and transwell invasion assay (TIA) for examining the migration and invasion of cells. The expressions of a set of genes related proliferation and migration were investigated with the Western Blotting (WB). RNA immunoprecipitation (RIP), biotinylated RNA pull-down and dual luciferase reporter tests were used to detect the interactions of RNU12 with miR-575/BLID. The in vivo proliferation and migration ability of RNU12 infected cells were determined in zebrafish system. This study revealed that RNU12 inhibited proliferation, invasion and metastasis by sponging of miR-575 and regulating the downstream BLID and modulated EMT of GC cells. The RNU12/miR-575/BLID axis is likely to be the prognosis biomarkers and drug targets of GC.
A new correlation belief function in Dempster-Shafer evidence theory and its application in classification
Authors: Tang Yongchuan , Zhang Xu , Zhou Ying , Huang Yubo , Zhou Deyun 
Published: May 2023

AbstractUncertain information processing is a key problem in classification. Dempster-Shafer evidence theory (D-S evidence theory) is widely used in uncertain information modelling and fusion. For uncertain information fusion, the Dempster’s combination rule in D-S evidence theory has limitation in some cases that it may cause counterintuitive fusion results. In this paper, a new correlation belief function is proposed to address this problem. The proposed method transfers the belief from a certain proposition to other related propositions to avoid the loss of information while doing information fusion, which can effectively solve the problem of conflict management in D-S evidence theory. The experimental results of classification on the UCI dataset show that the proposed method not only assigns a higher belief to the correct propositions than other methods, but also expresses the conflict among the data apparently. The robustness and superiority of the proposed method in classification are verified through experiments on different datasets with varying proportion of training set.
Boundary optimization of inclined coal seam open-pit mine based on the ISSA–LSSVR coal price prediction method
Authors: Cao Bo , Wang Shuai , Bai Runcai , Zhao Bo , Li Qingyi , Lv Mingjia , Liu Guangwei 
Published: May 2023

AbstractAs an important link in the complex system engineering project of open pit mining, the quality of the boundary determines the performance of the project to a large extent. However, changes in economic indicators may raise doubts about the optimality of mining boundaries. In this article, a coal price time series forecasting model that considers some amount of redundancy is proposed, which combines an improved sparrow search algorithm (ISSA) and a least squares support vector regression machine regression (LSSVR) algorithm. The optimal values of the penalty factor and kernel function parameter of the LSSVR model are selected by ISSA, which improves the prediction accuracy and generalization performance of the forecasting model. A multistep decision optimization method under fluctuating coal price conditions is proposed, and the model prediction results are applied to the boundary optimization design process. Using the widely applied block model as the basis, a set of optimal production nested pits is obtained, allowing the realm design results to fit the coal price fluctuation trend and further enhance enterprise efficiency. The applicability and effectiveness of this method were verified by taking an ideal two-dimensional model and an inclined coal seam open-pit coal mine in Xinjiang as an example.
Picoplankton diversity in an oligotrophic and high salinity environment in the central Adriatic Sea
Authors: Šantić Danijela , Stojan Iva , Matić Frano , Trumbić Željka , Vrdoljak Tomaš Ana , Fredotović Željana , Piwosz Kasia , Lepen Pleić Ivana , Šestanović Stefanija , Šolić Mladen 
Published: May 2023

AbstractBy combining qualitative 16S metabarcoding and quantitative CARD-FISH methods with neural gas analysis, different patterns of the picoplankton community were revealed at finer taxonomic levels in response to changing environmental conditions in the Adriatic Sea. We present the results of a one-year study carried out in an oligotrophic environment where increased salinity was recently observed. We have shown that the initial state of community structure changes according to environmental conditions and is expressed as qualitative and quantitative changes. A general pattern of increasing diversity under harsh environmental conditions, particularly under the influence of increasing salinity at the expense of community abundance was observed. Considering the trend of changing seawater characteristics due to climate change, this study helps in understanding a possible structural change in the microbial community of the Adriatic Sea that could affect higher levels of the marine food web.
Assessment of the incremental prognostic value from the modified frailty index-5 in complete traumatic cervical spinal cord injury
Authors: Shakil Husain , Jaja Blessing N. R. , Zhang Peng F. , Jaffe Rachael H. , Malhotra Armaan K. , Harrington Erin M. , Wijeysundera Duminda N. , Wilson Jefferson R. , Witiw Christopher D. 
Published: May 2023

AbstractFrailty, as measured by the modified frailty index-5 (mFI-5), and older age are associated with increased mortality in the setting of spinal cord injury (SCI). However, there is limited evidence demonstrating an incremental prognostic value derived from patient mFI-5. We conducted a retrospective cohort study to evaluate in-hospital mortality among adult complete cervical SCI patients at participating centers of the Trauma Quality Improvement Program from 2010 to 2018. Logistic regression was used to model in-hospital mortality, and the area under the receiver operating characteristic curve (AUROC) of regression models with age, mFI-5, or age with mFI-5 was used to compare the prognostic value of each model. 4733 patients were eligible. We found that both age (80 y versus 60 y: OR 3.59 95% CI [2.82 4.56], P < 0.001) and mFI-5 (score ≥ 2 versus < 2: OR 1.53 95% CI [1.19 1.97], P < 0.001) had statistically significant associations with in-hospital mortality. There was no significant difference in the AUROC of a model including age and mFI-5 when compared to a model including age without mFI-5 (95% CI Δ AUROC [− 8.72 × 10–4 0.82], P = 0.199). Both models were superior to a model including mFI-5 without age (95% CI Δ AUROC [0.06 0.09], P < 0.001). Our findings suggest that mFI-5 provides minimal incremental prognostic value over age with respect to in-hospital mortality for patients complete cervical SCI.
Data-driven automated control algorithm for floating-zone crystal growth derived by reinforcement learning
Authors: Tosa Yusuke , Omae Ryo , Matsumoto Ryohei , Sumitani Shogo , Harada Shunta 
Published: May 2023

AbstractThe complete automation of materials manufacturing with high productivity is a key problem in some materials processing. In floating zone (FZ) crystal growth, which is a manufacturing process for semiconductor wafers such as silicon, an operator adaptively controls the input parameters in accordance with the state of the crystal growth process. Since the operation dynamics of FZ crystal growth are complicated, automation is often difficult, and usually the process is manually controlled. Here we demonstrate automated control of FZ crystal growth by reinforcement learning using the dynamics predicted by Gaussian mixture modeling (GMM) from small numbers of trajectories. Our proposed method of constructing the control model is completely data-driven. Using an emulator program for FZ crystal growth, we show that the control model constructed by our proposed model can more accurately follow the ideal growth trajectory than demonstration trajectories created by human operation. Furthermore, we reveal that policy optimization near the demonstration trajectories realizes accurate control following the ideal trajectory.
Tuning the infrared resonance of thermal emission from metasurfaces working in near-infrared
Authors: Rasoga Oana , Dragoman Daniela , Dinescu Adrian , Dirdal Christopher Andrew , Zgura Irina , Nastase Florin , Baracu Angela Mihaela , Iftimie Sorina , Galca Aurelian Catalin 
Published: May 2023

AbstractWe simulated numerically and demonstrated experimentally that the thermal emittance of a metasurface consisting of an array of rectangular metallic meta-atoms patterned on a layered periodic dielectric structure grown on top of a metallic layer can be tuned by changing several parameters. The resonance frequency, designed to be in the near-infrared spectral region, can be tuned by modifying the number of dielectric periods, and the polarization and incidence angle of the incoming radiation. In addition, the absorbance/emittance value at the resonant wavelength can be tuned by modifying the orientation of meta-atoms with respect to the illumination direction.
Acute exercise as a modifier of neocortical plasticity and aperiodic activity in the visual cortex
Authors: Cadwallader Claire J. , Steiniger Jennifer , Cooper Patrick S. , Zhou Shou-Han , Hendrikse Joshua , Sumner Rachael L. , Kirk Ian J. , Chong Trevor T.-J. , Coxon James P. 
Published: May 2023

AbstractLong-term potentiation (LTP) is a form of neuroplasticity commonly implicated in mechanistic models of learning and memory. Acute exercise can boost LTP in the motor cortex, and is associated with a shift in excitation/inhibition (E:I) balance, but whether this extends to other regions such as the visual cortex is unknown. We investigated the effect of a preceding bout of exercise on LTP induction and the E:I balance in the visual cortex using electroencephalography (EEG). Young adults (N = 20, mean age = 24.20) engaged in 20 min of high-intensity interval training (HIIT) exercise and rest across two counterbalanced sessions. LTP was induced using a high frequency presentation of a visual stimulus; a “visual tetanus”. Established EEG markers of visual LTP, the N1b and P2 component of the visual evoked potential, and an EEG-derived measure of the E:I balance, the aperiodic exponent, were measured before and after the visual tetanus. As expected, there was a potentiation of the N1b following the visual tetanus, with specificity to the tetanised stimulus, and a non-specific potentiation of the P2. These effects were not sensitive to a preceding bout of exercise. However, the E:I balance showed a late shift towards inhibition following the visual tetanus. A preceding bout of exercise resulted in specificity of this E:I balance shift to the tetanised stimulus, that was not seen following rest. This novel finding suggests a possible exercise-induced tuning of the visual cortex to stimulus details following LTP induction.
Cortical suspensory button fixation has superior biomechanical properties to knotless anchor suture in anterior cruciate ligament repair: a biomechanical study
Authors: Itthipanichpong Thun , Thamrongskulsiri Napatpong , Tangpornprasert Pairat , Virulsri Chanyaphan , Limskul Danaithep , Kuptniratsaikul Somsak , Tanpowpong Thanathep 
Published: May 2023

AbstractThe purpose of our biomechanical study was to assess load-to-failure, stiffness, gap formation following cyclic loading, and the failure mechanism for anterior cruciate ligament (ACL) repair comparing the cortical suspensory button and knotless anchor suture. Eight Thiel’s embalmed paired cadaveric knees from four cadavers were dissected. The specimens were assigned to undergo ACL repair either with cortical suspensory button or with knotless anchor suture. The Instron machine replicates cyclic loading and then determines the gap formation. Traction was applied until failure. The load-to-failure, stiffness, and modes of failure in both groups were recorded. The load-to-failure, stiffness, and gap formation were compared between the two groups using the student's t-test. The mean load-to-failure in the cortical suspensory button group was significantly higher than the knotless anchor suture group (212.96 ± 54.57 vs 44.57 ± 20.80, p value < 0.01). No statistically significant difference was found regarding gap formation following cyclic loading and stiffness between the cortical suspensory button group and the knotless anchor suture group. This biomechanical study showed a higher load-to-failure for the ACL repair with cortical suspensory button compared to ACL repair with knotless anchor suture, while no statistically significant difference was found regarding the gap formation following cyclic loading and the stiffness. The load-to-failure in both cortical suspensory button and knotless anchor suture are below regular daily activity load. Thus, an internal brace or external support is recommended during rehabilitation.
A prognostic model based on the Augmin family genes for LGG patients
Authors: Wang Tao , Yao Senbang , Li Siyu , Fei Xichang , Zhang Mingjun 
Published: May 2023

AbstractGliomas are the most prevalent primary tumors in the central nervous system. Despite some breakthroughs in the treatment of glioma in recent years, survival rates remain low. Although genes of the Augmin family play a key role in microtubule nucleation, the role they play in gliomas is unclear. Transcriptome data were extracted from UCSC XENA and GTEx for low-grade glioma (LGG) and normal tissues, respectively. The protein interaction network associated with Augmin family genes was established using STRING and GeneMANIA databases. Enrichment analysis of gene-related functions and pathways was used to explore potential biological pathways and TIMER to assess immune cell infiltration. Regression analysis and Kaplan–Meier analysis were used to look at the clinical characteristics of the Augmin family genes and the association with the prognosis of patients with glioma. The results showed that the mRNA expression of Augmin family genes was significantly elevated in LGG tissues, except for HAUS7. Immunoregulation, cell cycle, apoptosis and other signaling pathways may be involved in the development and progression of LGG. Except for HAUS4 and HAUS7, the expression of all genes was positively correlated with immune cell infiltration. High expression of HAUS1, HAUS3, HAUS5, HAUS7, HAUS8 and low expression of HAUS4, HAUS6 in LGG was associated with poor prognosis. The risk models constructed based on the pivotal genes HAUS2, HAUS4 and HAUS8 were validated by nomogram and confirmed to be clinically useful for predicting the prognosis of LGG.
Microbiome analysis of thai traditional fermented soybeans reveals short-chain fatty acid-associated bacterial taxa
Authors: Wongsurawat Thidathip , Sutheeworapong Sawannee , Jenjaroenpun Piroon , Charoensiddhi Suvimol , Khoiri Ahmad Nuruddin , Topanurak Supachai , Sutthikornchai Chantira , Jintaridth Pornrutsami 
Published: May 2023

AbstractThua Nao is a Thai traditional fermented soybean food and low-cost protein supplement. This study aimed to evaluate the bacterial community in Thua Nao from northern Thailand and assess potentially active short-chain fatty acids (SCFAs)-related bacteria. Sixty-five Thua Nao consisting of 30 wet and 35 dried samples were collected from six provinces: Chiang Rai, Chiang Mai, Mae Hong Son, Lampang, Lamphun, and Phayao. Bacterial diversity was significantly higher in the wet samples than in the dried samples. The dominant phyla were Firmicutes (92.7%), Proteobacteria (6.7%), Actinobacteriota (0.42%), and Bacteroidota (0.26%). The genus Bacillus (67%) was the most represented in all samples. Lactobacillus, Enterococcus, and Globicatella were enriched in the wet samples. Assessment of the SCFA-microbiota relationships revealed that high butyrate and propionate concentrations were associated with an increased Clostridiales abundance, and high acetate concentrations were associated with an increased Weissella abundance. Wet products contained more SCFAs, including acetate (P = 2.8e−08), propionate (P = 0.0044), butyrate (P = 0.0021), and isovalerate (P = 0.017), than the dried products. These results provide insight into SCFA-microbiota associations in Thua Nao, which may enable the development of starter cultures for SCFA-enriched Thua Nao production.
Valence state control of Cr4+-activated Li2SrGeO4for NIR-II light source to distinguish deuterium and non-deuterium reagents
Authors: Guo Xiaoxuan , Liu Bomei , Kuang Rongyi , Gan Weijiang , Huang Lin , Wang Jing 
Published: May 2023

Li2SrGeO4:Cr4+ phosphors were prepared as NIR-II light source for distinguishing H2O/D2O.
A novel quasi‐hot standby fault‐tolerant control strategy for cascaded H‐bridge rectifier
Authors: Liao Xin , Wang Shunliang , Jiao Ning , Zhang Rui , Ma Junpeng , Liu Tianqi 
Published: May 2023

AbstractCascaded H‐bridge rectifier (CHBR) is widely used in high‐power fields such as STATCOM and power electric traction transformer (PETT) due to its high efficiency and easy expandability. With the high‐reliability operation requirements of power equipment, the fault‐tolerance capability has gradually become a research hotspot nowadays. In order to improve the reliability of the power supply, cascaded H‐bridge rectifier should restore as soon as possible after a fault. However, the traditional fault‐tolerant strategies have a great effect on the grid side and a long recovery time. This paper proposed a quasi‐hot standby fault‐tolerant control strategy. Compared with the traditional strategies, the quasi‐hot standby fault‐tolerant control strategy has a faster recovery speed after the malfunction, and its transient recovery process has less impact on the power grid. Simulation and experiment results show the effectiveness and good recovery performance of the proposed fault‐tolerant control strategy.