bluuebunny/crossref_metadata_2025_split huggingface dataset:
Vegetable and fruit consumption during the COVID-19 lockdown: eating habits in Ecuador
Authors: Andrade-Cuvi Maria Jose , Montalvo Cesar , Moreno-Guerrero Carlota 
Published: September 2023

AbstractThis study identifies changes in fruit and vegetable (FV) consumption habits during the COVID-19 pandemic in Quito-Ecuador and observe relationships regarding household income and the relevance of FV nutritional value. The study used an online survey among urban residents (n = 1093) in Quito-Ecuador, regarding FV consumption patterns during the lockdown along with household socioeconomic status. A multiple response logistic model was used to analyse changes in FV eating habits across four income categories. Income had a statistically significant effect over the healthy food consumption of FV for the low- and high-income category. Families did not change eating habits nor decide to maintain a diet based on differences of income. Further, approximately two thirds of the families increased FV consumption during the lockdown. Eating habits changed during the lockdown of urban households in Ecuador. Income explained changes in FV consumption for low- and high-income households but did not for the middle-income group. Households that purchased more FV had a higher perception of their own healthy eating habits. The consumption of FV with high content of vitamin C increased, however, families did not purchase products with the highest vitamin-C content.
Adaption of the meat attachment scale (MEAS) to Germany: interplay with food neophobia, preference for organic foods, social trust and trust in food technology innovations
Authors: Kühn David , Profeta Adriano , Krikser Thomas , Heinz Volker 
Published: September 2023

AbstractMeat-based diets are still the norm, and vegans and vegetarians represent only a small minority of the population. A transition, respectively, behavioural change towards a diet with less meat can only occur by adopting a positive attitude towards dietary changes based on reasons and motivations. The main aim of this study is to apply the meat attachment scale (MEAS) in Germany in order to analyse if this construct is a barrier towards a diet with less meat in this country. For this purpose, the impact of meat attachment on the trust in different protein alternatives (plant-based, insects, cultured meat) and related food processing technologies is analysed. The findings reveal that a high level of meat attachment goes along with lower trust in plant-based proteins. Similar holds for cultured meat and insect proteins. Thus it appears that, at least for the moment, cultured meat or proteins from insects are not a logical substitute for the heavily meat attached consumer. Furthermore, in the analysis, we considered if meat attachment as measured by the MEAS is correlated with other scales/preferences such as food neophobia, social trust, and attitude towards respective preference for organic products. Literature shows that all mentioned constructs impact the acceptance, preference or trust in more sustainable food product innovation, respectively, more sustainable food processing techniques. The outcome of the correlation analysis demonstrated that in particular food neophobia and meat attachment are not correlated with each other. That is, the MEAS represents a predictor for trust in food (processing) technologies as e.g. plant-based proteins or cultured meat that is independent of the neophobia construct.
Circulating cell-free DNA (cfDNA) in patients with medullary thyroid carcinoma is characterized by specific fragmentation and methylation changes with diagnostic value
Authors: Citarella Anna , Besharat Zein Mersini , Trocchianesi Sofia , Autilio Tanja Milena , Verrienti Antonella , Catanzaro Giuseppina , Splendiani Elena , Spinello Zaira , Cantara Silvia , Zavattari Patrizia , Loi Eleonora , Romei Cristina , Ciampi Raffaele , Pezzullo Luciano , Castagna Maria Grazia , Angeloni Antonio , Elisei Rosella , Durante Cosimo , Po Agnese , Ferretti Elisabetta 
Published: September 2023

AbstractMedullary Thyroid Carcinoma (MTC) is a rare neuroendocrine tumour whose diagnosis includes evaluating calcitonin serum levels, which can present fluctuations unrelated to MTC. Here, we investigated circulating DNA fragmentation and methylation changes as potential biomarkers using ddPCR on cell-free DNA (cfDNA) isolated from the plasma of MTC patients. For cfDNA fragmentation analysis, we investigated the fragment size distribution of a gene family and calculated short fragment fraction (SFF). Methylation analyses evaluated the methylation levels of CG_16698623, a CG dinucleotide in the MGMT gene that we found hypermethylated in MTC tissues by analyzing public databases. The SFF ratio and methylation of CG_16698623 were significantly increased in plasma from MTC patients at diagnosis, and patients with clinical remission or stable disease at follow-up showed no significant SFF difference compared with healthy subjects. Our data support the diagnostic value of cfDNA traits that could enable better management of MTC patients.
New insights into the use of Neolithic pottery in Guangxi of South China: organic residue analysis of experimental and archaeological pottery
Authors: Liu Xiaodi , Ren Meng , Fu Yongxu , Hu Yaowu , Wang Shuzhi , Yang Yimin 
Published: September 2023

Abstract
As one of the world’s earliest pottery origins in Eurasia, the role of pottery during Neolithic period in South China remains unclear. Here, we present a new understanding on the utilization pattern of early pottery from the Neolithic sites of Dayan, Dingsishan and Zengpiyan in Guangxi province, China. Compound-specific stable isotope analysis of pottery and bone lipids show that a persistent use of terrestrial C3 and C4 resources throughout the pottery utilization history highlight the culinary practice and terrestrial exploitation during Neolithic period. Contrary to the traditional view that the primary use of these pottery was closely linked with aquatic resource processing, no aquatic biomarkers were found in the potsherds, yet these compounds were detected in the cooking experiments in this study, indicating that aquatic resources in these sites were probably processed or consumed in other ways. Moreover, a remarkable diachronic change from extensive use of C3 and C4 resources to the intensive use of certain kinds of C3 resources in different Neolithic stages, further indicating that an ecological tradition rooted in the subtropical and tropical environments as well as the Neolithic social evolution exerted great influence upon the initial production and the subsequent utilization of early pottery in prehistoric South China.
Investigating the educational experiences of students with disabilities during the COVID-19 school disruption: an international perspective
Authors: Shelton Alexandra , Gezer Tuba 
Published: September 2023

AbstractStudents with disabilities generally experience educational inequities around the world. The coronavirus 2019 (COVID-19) pandemic likely exacerbated these inequities in access, resources, and support as schools shut down to mitigate the spread of the disease. Although some research has explored disparities between students with and without disabilities during the pandemic, limited research has explored this issue from the perspective of students across multiple countries. Therefore, we conducted a secondary analysis of the UNESCO Responses to Educational Disruption Survey student questionnaire administered to eighth graders in five countries to investigate changes in the educational experiences of students with disabilities during COVID-19 school disruptions and differences between these experiences and the experiences of students without disabilities during these disruptions. Specifically, we aimed to understand how students with disabilities’ perceptions of their educational experiences changed during disruptions and varied from those of students without disabilities. Contrary to previous research, our findings revealed that students with disabilities generally reported positive experiences to a greater extent than students without disabilities. We discuss the implications of these findings and areas for future research beyond the COVID-19 pandemic.
Ultrasonographic evaluation of the diaphragm in critically ill patients to predict invasive mechanical ventilation
Authors: Suttapanit Karn , Wongkrasunt Supawit , Savatmongkorngul Sorravit , Supatanakij Praphaphorn 
Published: September 2023

Abstract
Background
Diaphragm dysfunction is common in critically ill patients and associated with poorer outcomes. The function of the diaphragm can be evaluated at the bedside by measuring diaphragmatic excursion using ultrasonography. In this study, we investigated the ability of right-sided diaphragmatic excursion (RDE) to predict the need for invasive mechanical ventilation (IMV).

Methods
Critically ill patients aged 18 years and older who presented to our emergency department between May 20, 2021 and May 19, 2022 and underwent measurement of RDE within 10 min of arrival were enrolled in this prospective study. The ability of RDE to predict the need for IMV was assessed by multivariable logistic regression and analysis of the area under the receiver-operating characteristic curve (AUROC).

Results
A total of 314 patients were enrolled in the study; 113 (35.9%) of these patients required IMV. An increase of RDE value per each 0.1 cm was identified to be an independent predictor of IMV (adjusted odds ratio 0.08, 95% confidence interval [CI] 0.04–0.17, p < 0.001; AUROC 0.850, 95% CI 0.807–0.894). The RDE cutoff value was 1.2 cm (sensitivity 82.3%, 95% CI 74.0–88.8; specificity 78.1%, 95% CI 71.7–83.6). Time on a ventilator was significantly longer when the RDE was ≤ 1.2 cm (13 days [interquartile range 5, 27] versus 5 days [interquartile range 3, 8], p = 0.006).

Conclusions
In this study, RDE had a good ability to predict the need for IMV in critically ill patients. The optimal RDE cutoff value was 1.2 cm. Its benefit in patient management requires further investigation.
Effect of fatty acids on intracellular pneumocandin B0 storage in the fermentation of Glarea lozoyensis
Authors: Zhang Weiting , Yi Ping , Zhou Ying , Yuan Kai , Ji Xiaojun , Song Ping 
Published: September 2023

AbstractThe natural product pneumocandin B0 is the precursor of the antifungal drug caspofungin. To explore the relationship between pneumocandin B0 and oil. We found that the addition of 1 g/L of oil to the fermentation medium is more conducive to the production of pneumocandin B0. The metabolic reaction mechanism was explored using different fatty acids and the results showed that stearic acid and acetic acid increased the total production of pneumocandin B0 by 22.98% and 9.08%, respectively, as well as increasing the content of intracellular lipid droplets. We also analyzed gene expression and pathway differences between the two different fatty acids using transcriptome analyses. The addition of both acetic acid and stearic acid promoted an active pentose phosphate pathway, providing cells with higher intracellular reducing power. We found that the addition of fatty acids can lead to lipid accumulation, and lipid droplets can sequester lipophilic secondary metabolites such as pneumocandin B0 to reduce cell damage. These results provide novel insights into the relationship between pneumocandin B0 biosynthesis and fatty acids in G. lozoyensis. In addition, this study provides important genetic information for improving the yield of pneumocandin B0 through a strategy of metabolic engineering in the future.
Graphical Abstract
Evaluation of the clinical safety and performance of a narrow diameter (2.9 mm) bone-level implant: a 1-year prospective single-arm multicenter study
Authors: Walter Christian , Sagheb Keyvan , Blatt Sebastian , Klein Marcus Oliver , Herrmann Jan , Kleinheinz Johannes , Al-Nawas Bilal 
Published: September 2023

Abstract
Purpose
Narrow-diameter implants facilitate single‐tooth restoration when interdental or inter-implant spaces and bone volume are inadequate for using standard diameter implants. This study reports the short-term data on the clinical safety and performance of a bone-level-tapered two-piece implant with a 2.9 mm diameter in the clinical practice setting. This study was retrospectively registered on March 1st, 2016 (NCT02699866).

Methods
Implants were placed in partially healed extraction sockets of the central and lateral incisors in the mandible and lateral incisors in the maxilla for single-tooth replacement. The primary outcome was to assess implant survival at 12 months after placement. Secondary outcomes included implant success, pink esthetic score, marginal bone-level changes, and safety.

Results
Twenty four males and 17 females with a mean age of 44.5 (± 18.3 standard deviation) received the implant. Three out of 41 implants were lost yielding a survival rate of 92.7% (95%-CI: 79.0%; 97.6%) at 1 year. One patient reported an ongoing foreign body sensation, pain, and/or dysesthesia at month 12. The average pink esthetic score at 6 months was 11.2 (95%-CI: 10.5; 11.9). The bone level was stable with a mean bone-level change of—0.3 mm (± 0.42 mm standard deviation) at 1 year after implantation. No serious adverse events or adverse device events were reported.

Conclusions
The use of a 2.9 mm diameter bone-level-tapered implant is a safe and reliable treatment option for narrow tooth gaps at the indicated locations. Overall performance and good survival rates support their use in cases, where wider implants are unsuitable.

Graphical abstract
P1NP and β-CTX-1 Responses to a Prolonged, Continuous Running Bout in Young Healthy Adult Males: A Systematic Review with Individual Participant Data Meta-analysis
Authors: Civil Rita , Dolan Eimear , Swinton Paul A. , Santos Lívia , Varley Ian , Atherton Philip J. , Elliott-Sale Kirsty J. , Sale Craig 
Published: September 2023

Abstract
Background
Circulating biomarkers of bone formation and resorption are widely used in exercise metabolism research, but their responses to exercise are not clear. This study aimed to quantify group responses and inter-individual variability of P1NP and β-CTX-1 after prolonged, continuous running (60–120 min at 65–75% V̇O2max) in young healthy adult males using individual participant data (IPD) meta-analysis.

Methods
The protocol was designed following PRISMA-IPD guidelines and was pre-registered on the Open Science Framework prior to implementation (https://osf.io/y69nd). Changes in P1NP and β-CTX-1 relative to baseline were measured during, immediately after, and in the hours and days following exercise. Typical hourly and daily variations were estimated from P1NP and β-CTX-1 changes relative to baseline in non-exercise (control) conditions. Group responses and inter-individual variability were quantified with estimates of the mean and standard deviation of the difference, and the proportion of participants exhibiting an increased response. Models were conducted within a Bayesian framework with random intercepts to account for systematic variation across studies.

Results
P1NP levels increased during and immediately after running, when the proportion of response was close to 100% (75% CrI: 99 to 100%). P1NP levels returned to baseline levels within 1 h and over the next 4 days, showing comparable mean and standard deviation of the difference with typical hourly (0.1 ± 7.6 ng·mL−1) and daily (− 0.4 ± 5.7 ng·mL−1) variation values. β-CTX-1 levels decreased during and up to 4 h after running with distributions comparable to typical hourly variation (− 0.13 ± 0.11 ng·mL−1). There was no evidence of changes in β-CTX-1 levels during the 4 days after the running bout, when distributions were also similar between the running data and typical daily variation (− 0.03 ± 0.10 ng·mL−1).

Conclusion
Transient increases in P1NP were likely biological artefacts (e.g., connective tissue leakage) and not reflective of bone formation. Comparable small decreases in β-CTX-1 identified in both control and running data, suggested that these changes were due to the markers’ circadian rhythm and not the running intervention. Hence, prolonged continuous treadmill running did not elicit bone responses, as determined by P1NP and β-CTX-1, in this population.
Cluster Sets to Prescribe Interval Resistance Training: A Potential Method to Optimise Resistance Training Safety, Feasibility and Efficacy in Cardiac Patients
Authors: Way Kimberley L. , Thomas Hannah J. , Parker Lewan , Maiorana Andrew , Keske Michelle A. , Scott David , Reed Jennifer L. , Tieng Jessica , Hackett Daniel , Hawkins Tess , Latella Christopher , Cordina Rachael , Tran Derek L. 
Published: September 2023

AbstractThe integration of resistance training for cardiac patients leads to important health outcomes that are not optimally obtained with aerobic exercise; these include an increase in muscle mass, maintenance of bone mineral density, and improvements in muscular fitness parameters. Despite the proliferation of evidence supporting resistance exercise in recent decades, the implementation of resistance training is underutilised, and prescription is often sub-optimal in cardiac patients. This is frequently associated with safety concerns and inadequate methods of practical exercise prescription. This review discusses the potential application of cluster sets to prescribe interval resistance training in cardiac populations. The addition of planned, regular passive intra-set rest periods (cluster sets) in resistance training (i.e., interval resistance training) may be a practical solution for reducing the magnitude of haemodynamic responses observed with traditional resistance training. This interval resistance training approach may be a more suitable option for cardiac patients. Additionally, many cardiac patients present with impaired exercise tolerance; this model of interval resistance training may be a more suitable option to reduce fatigue, increase patient tolerance and enhance performance to these workloads. Practical strategies to implement interval resistance training for cardiac patients are also discussed. Preliminary evidence suggests that interval resistance training may lead to safer acute haemodynamic responses in cardiac patients. Future research is needed to determine the efficacy and feasibility of interval resistance training for health outcomes in this population.
Reproductive characteristics of the hermaphroditic four-finger threadfin, Eleutheronema tetradactylum (Shaw, 1804), in tropical coastal waters
Authors: Soe Kay Khine , Iqbal Teuku Haris , Lim Apiradee , Wang Wen‑Xiong , Tsim Karl W. K. , Takeuchi Yutaka , Petchsupa Nirattisai , Hajisamae Sukree 
Published: September 2023

AbstractThis study investigated the reproductive traits of the hermaphroditic four-finger threadfin,Eleutheronema tetradactylum, along the coasts of Thailand during January to December 2021. Fish samples were collected from Pattani Bay, Thailand to assess the sex ratio, gonadosomatic index (GSI), maturity stage and fecundity. Additional fish samples were also collected from other areas to evaluate the length and weight at first sex change (Ls50and Ws50) and length at first maturity (Lm50). The overall sex ratio for male and female was 1:0.69 with male being predominant throughout the year. Threadfin fish spawn the whole year round with peaks during moderate rainy and heavy rainy seasons. Histological examination confirmed its protandrous hermaphrodite posing multiple spawning habits. The average fecundity was 1.85 × 105 ± 1.05 × 105eggs and positively related with standard length, body weight, gonad weight, and egg diameter (p < 0.05). The Ls50and Ws50were 27.58 cm and 419.39 g, and 29.71 cm and 457.28 g, for fish from Pattani Bay and Samut Prakan province, respectively. The Lm50of male from Pattani Bay and Samut Prakan province were 25.78 cm and 25.56 cm, respectively, which were larger than those from Satun and Nakhon Sri Thammarat provinces. The Lm50of females from Pattani Bay was smaller than that from Samut Prakan province. This study provided fundamental information on the reproductive characteristics ofE. tetradactylum, which can be implemented to support management of natural fish stock and aquaculture development.
Radiologic simulation of leg length change after double level osteotomy in preoperative surgical planning
Authors: Nejima Shuntaro , Kumagai Ken , Yamada Shunsuke , Sotozawa Masaichi , Inaba Yutaka 
Published: September 2023

Abstract
Background
To evaluate the expected postoperative total leg length change using preoperative radiographs during surgical planning of four different methods of double level osteotomy (DLO).

Methods
This study included 34 patients (44 knees) who underwent DLO for varus knee osteoarthritis. Surgical planning was performed so that the postoperative weight bearing line ratio was 62.5%. In DLO, lateral closed or medial open wedge distal femoral osteotomy (LCWDFO, MOWDFO) was performed so that the postoperative mechanical lateral distal femoral angle was 85°, and residual deformity was corrected with medial open or lateral closed wedge high tibial osteotomy (MOWHTO, LCWHTO). Pre- and surgical planning X-rays in the one-leg standing position were compared to assess the change in leg length, and the factors affecting it, in the various surgical groups. The proportion of cases in which Δ total leg length was greater than 6 mm (symptomatic change) was investigated.

Results
The mean postoperative total leg length increased significantly with LCWDFO + MOWHTO, MOWDFO + MOWHTO, and MOWDFO + LCWHTO, while it decreased with LCWDFO + LCWHTO. The proportion of cases with a postoperative total leg length change > 6 mm was 72.7%, 2.3%, 100%, and 6.8% in LCWDFO + MOWHTO, LCWDFO + LCWHTO, MOWDFO + MOWHTO, and MOWDFO + LCWHTO, respectively. In addition, the preoperative hip-knee-ankle angle correlated negatively with the postoperative total leg length change in LCWDFO + MOWHTO, MOWDFO + MOWHTO, and MOWDFO + LCWHTO, but not in LCWDFO + LCWHTO.

Conclusions
MOWDFO + MOWHTO had the largest postoperative leg length change and MOWDFO + LCWHTO had the smallest. Symptomatic leg length change (> 6 mm) should be considered in MOWDFO + MOWHTO and LCWDFO + MOWHTO.
Classifying blinking and winking EOG signals using statistical analysis and LSTM algorithm
Authors: Hassanein Ahmed M. D. E. , Mohamed Ahmed G. M. A. , Abdullah Mohamed A. H. M. 
Published: September 2023

AbstractDetection of eye movement types whether the movement of the eye itself or blinking has attracted a lot of recent research. In this paper, one method to detect the type of wink or blink produced by the eye is scrutinized and another method is proposed. We discuss what statistical analysis can teach us about detection of eye movement and propose a method based on long short-term memory (LSTM) networks to detect those types. The statistical analysis is composed of two main steps, namely calculation of the first derivative followed by a digitization step. According to the values of the digitized curve and the duration of the signal, the type of the signal is detected. The success rate reached 86.6% in detection of the movement of the eye when those volunteers are not trained on using our system. However, when they are trained, the detection success rate reached 93.3%. The statistical analysis succeeds in achieving detection of all types of eye movement except one type which is the non-intentional blinking. Although rate of success achieved is high, but as the number of people using this system increases, the error in detection increases that is because it is fixed and not adaptive to changes. However; we learnt from statistical analysis that the first derivative is a very important feature to classify the type of an EOG signal. Next, we propose using the LSTM network to classify EOG signals. The effect of using the first derivative as a feature for identifying the type of EOG signals is discussed. The LSTM algorithm succeeds in detecting the type of EOG signals with a percentage equal to 92% for all types of eye movement.