bluuebunny/crossref_metadata_2025_split huggingface dataset:
Phylogenomics reveals widespread hybridization and polyploidization in Henckelia (Gesneriaceae)
Authors: Yang Li-Hua , Shi Xi-Zuo , Wen Fang , Kang Ming 
Published: May 2023

Abstract

Background and Aims
Hybridization has long been recognized as an important process for plant evolution and is often accompanied by polyploidization, another prominent force in generating biodiversity. Despite its pivotal importance in evolution, the actual prevalence and distribution of hybridization across the tree of life remain unclear.

Methods
We used whole-genome shotgun (WGS) sequencing and cytological data to investigate the evolutionary history of Henckelia, a large genus in the family Gesneriaceae with a high frequency of suspected hybridization and polyploidization events. We generated WGS sequencing data at about 10× coverage for 26 Chinese Henckelia species plus one Sri Lankan species. To untangle the hybridization history, we separately extracted whole plastomes and thousands of single-copy nuclear genes from the sequencing data, and reconstructed phylogenies based on both nuclear and plastid data. We also explored sources of both genealogical and cytonuclear conflicts and identified signals of hybridization and introgression within our phylogenomic dataset using several statistical methods. Additionally, to test the polyploidization history, we evaluated chromosome counts for 45 populations of the 27 Henckelia species studied.

Key Results
We obtained well-supported phylogenetic relationships using both concatenation- and coalescent-based methods. However, the nuclear phylogenies were highly inconsistent with the plastid phylogeny, and we observed intensive discordance among nuclear gene trees. Further analyses suggested that both incomplete lineage sorting and gene flow contributed to the observed cytonuclear and genealogical discordance. Our analyses of introgression and phylogenetic networks revealed a complex history of hybridization within the genus Henckelia. In addition, based on chromosome counts for 27 Henckelia species, we found independent polyploidization events occurred within Henckelia after different hybridization events.

Conclusions
Our findings demonstrated that hybridization and polyploidization are common in Henckelia. Furthermore, our results revealed that H. oblongifolia is not a member of the redefined Henckelia and they suggested several other taxonomic treatments in this genus.
Successful tapering of dupilumab in patients with atopic dermatitis with low disease activity: a large pragmatic daily practice study from the BioDay registry
Authors: Spekhorst Lotte S , Boesjes Celeste M , Loman Laura , Zuithoff Nicolaas P A , Bakker Daphne S , Kamphuis Esmé , Kamsteeg Marijke , Haeck Inge M , Oosting Albert J , van Lumig Paula P M , van Lynden-van Nes Anneke M T , Tupker Ron A , Flinterman Annebeth , Garritsen Floor M , Touwslager Wouter R H , de Bruin-Weller Marjolein S , Schuttelaar Marie-Louise A , de Graaf Marlies 
Published: May 2023

Abstract

Background
Limited data are available regarding patient-centred dosing of dupilumab for atopic dermatitis (AD) in daily practice.

Objectives
To evaluate our patient-centred dupilumab dosing regimen in daily practice, to assess prognostic factors for successful tapering and to estimate medication-related cost savings.

Methods
This prospective multicentre study included adult patients with AD, participating in the BioDay registry, treated with dupilumab for ≥ 1.3 years. Interval prolongation was considered in the case of dupilumab standard dose for ≥ 1 year and persistent controlled AD [Eczema Area and Severity Index (EASI) ≤ 7; ≥ 6 months]. Primary endpoints were the mean EASI and Numeric Rating Scale (NRS)-pruritus after the start of tapering. Prognostic factors for successful tapering were analysed with logistic regression and a cost-savings analysis was performed.

Results
A total of 595 patients were included, of whom 401 patients [mean EASI 2.5 (SD 2.3); NRS-pruritus of 2.4 (SD 1.9) at the start of tapering] prolonged their dupilumab interval. In 83.3% of these patients tapering was successful; most patients used dupilumab every 3 or 4 weeks (Q3W/Q4W). A significant small increase was observed for EASI (highest mean 3.5) and NRS-pruritus (highest mean 3.2) (P &lt; 0.001); however, scores remained low. Predicting successful tapering showed nonsignificant odds ratios for all incorporated variables. The estimated cost savings was €3 977 033.98 for 401 patients between January 2019 and June 2022.

Conclusions
This study showed successful tapering of dupilumab in 83.3% of patients with AD who attempted tapering, while maintaining controlled disease and with the majority using Q3W/Q4W. Interval prolongation can be beneficial both for the patient and from a socio-economic perspective.
Breast carcinoma detection in ex vivo fresh human breast surgical specimens using a fast slide-free confocal microscopy scanner: HIBISCUSS project
Authors: Conversano Angelica , Abbaci Muriel , van Diest Paul , Roulot Aurélie , Falco Giuseppe , Ferchiou Malek , Coiro Saverio , Richir Milan , Genolet Pierre-Michel , Clement Carine , Casiraghi Odile , Lahkdar Aicha Ben , Labaied Nizard , Ragazzi Moira , Mathieu Marie-Christine 
Published: May 2023

Abstract

Background
New generation ultra-fast fluorescence confocal microscopy allows the ex vivo intraoperative analysis of fresh tissue. The High resolution Imaging for Breast carcInoma detection in ex vivo Specimens after breast Conserving sUrgery by hiStolog Scanner (HIBISCUSS) project aimed to develop an online learning program to recognize the main breast tissue features on ultra-fast fluorescence confocal microscopy images and to evaluate the performance of surgeons and pathologists in diagnosing cancerous and non-cancerous breast tissue in ultra-fast fluorescence confocal microscopy images.

Methods
Patients who underwent conservative surgery or mastectomy for breast carcinoma (invasive or in situ lesions) were included. The fresh specimens were stained with a fluorescent dye and imaged using a large field-of-view (20 cm2) ultra-fast fluorescence confocal microscope.

Results
One hundred and eighty-one patients were included. The images from 55 patients were annotated to generate learning sheets and images from 126 patients were blindly interpreted by seven surgeons and two pathologists. The time for tissue processing and ultra-fast fluorescence confocal microscopy imaging was between 8 and 10 min. The training program was composed of 110 images divided into nine learning sessions. The final database for blind performance assessment comprised 300 images. The mean duration for one training session and one performance round was 17 and 27 min respectively. The performance of pathologists was almost perfect with 99.6 per cent (standard deviation (s.d.) 5.4 per cent) accuracy. Surgeons’ accuracy significantly increased (P = 0.001) from 83 per cent (s.d. 8.4 per cent) in round 1 to 98 per cent (s.d. 4.1 per cent) in round 7 as well as the sensitivity (P = 0.004). Specificity increased without significance from 84 per cent (s.d. 16.7 per cent) in round 1 to 87 per cent (s.d. 16.4 per cent) in round 7 (P = 0.060).

Conclusion
Pathologists and surgeons showed a short learning curve in differentiating breast cancer from non-cancerous tissue in ultra-fast fluorescence confocal microscopy images. Performance assessment for both specialties supports ultra-fast fluorescence confocal microscopy evaluation for intraoperative management.

Registration number
NCT04976556 (http://www.clinicaltrials.gov).
Within-field spatial patterns of Euschistus servus and Nezara viridula (Hemiptera: Pentatomidae) in field corn
Authors: Bryant Tim B , Greene Jeremy K , Reay-Jones Francis P F 
Published: May 2023

Abstract
A complex of stink bugs, primarily the brown stink bug, Euschistus servus (Say) (Hemiptera: Pentatomidae) , and the southern green stink bug, Nezara viridula (L.) (Hemiptera: Pentatomidae) , are the most damaging insect pests of field corn, Zea mays L., in the southeastern United States. Characterizing the spatial patterns of these highly mobile, polyphagous pests is critical for developing efficient and effective sampling plans. In 2021 and 2022, stink bugs and their injury were assessed biweekly from emergence through R2 in 20 corn fields. The spatial analysis by distance indices (SADIE) showed that aggregation patterns were identified primarily in adult populations of both E. servus and N. viridula, and in nymphal populations of both species to a lesser extent. Aggregation patterns were also identified in early vegetative injury, but not in ear injury assessed at R2. The spatial association of stink bugs and their injury varied with corn phenological stage. A lack of spatial association between stink bug populations early in the season and vegetative injury suggests a need for intensive sampling, particularly in fields with increased residue from cover crops. Results of this study illustrate the variability in spatial patterns of stink bugs in corn, which can help to improve sampling plans for decision-making in IPM programs.
Sodium-glucose cotransporter-2 inhibitors compared with glucagon-like-peptide-1 receptor agonists and out-of-hospital cardiac arrest in type 2 diabetes: a nationwide nested case-control study
Authors: Júlíusdóttir Yrsa Kolka , Halili Andrim , Coronel Ruben , Folke Fredrik , Torp-Pedersen Christian , Gislason Gunnar Hilmar , Eroglu Talip E 
Published: May 2023

Abstract

Aims
Sodium-glucose cotransporter-2 inhibitors (SGLT-2is) are antidiabetic drugs that have beneficial direct effects on the myocardium by impacting cardiac ion channels and exchangers that control cardiac electrophysiology. We investigated the relationship between SGLT-2is in comparison to glucagon-like peptide-1 receptor agonists (GLP-1as) and out-of-hospital cardiac arrest (OHCA) in individuals with type 2 diabetes.

Methods
Using data from Danish registries, we conducted a nationwide nested case-control study in a cohort of individuals with type 2 diabetes between 2013 and 2019. Cases were defined as OHCA victims from presumed cardiac causes and each case was randomly matched with five controls without OHCA based on age, sex, and index-date (OHCA date). Conditional logistic regression models were used to estimate the adjusted odds ratios (ORs) with 95% confidence interval (95% CI) of OHCA comparing SGLT-2i use with GLP-1as (reference).

Results
The study population consisted of 3618 OHCA cases and 18 090 matched controls. SGLT-2i was used by 91 cases and 593 controls, and was associated with reduced odds of OHCA compared with use of GLP-1a after controlling for the relevant confounders (adjusted OR 0.76 [95% CI:0.58–0.99]). The adjusted OR of OHCA associated with SGLT-2i use did not vary significantly by sex (P-value interaction: 0.461), pre-existing cardiac disease (P-value interaction: 0.762), heart failure (P-value interaction: 0.891), diabetes duration (P-value interaction: 0.101), and chronic kidney disease (P-value interaction: 0.894).

Conclusion
Use of SGLT-2i is associated with a reduced risk of OHCA compared with use of GLP-1a in type 2 diabetes.
Sodium–glucose cotransporter 2 inhibitor use in early-phase acute coronary syndrome with severe heart failure
Authors: Kanaoka Koshiro , Iwanaga Yoshitaka , Nakai Michikazu , Nishioka Yuichi , Myojin Tomoya , Kubo Shinichiro , Okada Katsuki , Noda Tatsuya , Sakata Yasushi , Miyamoto Yoshihiro , Saito Yoshihiko , Imamura Tomoaki 
Published: May 2023

Abstract

Aims
Sodium–glucose cotransporter 2 inhibitor (SGLT2i) improves clinical outcomes in patients with heart failure (HF), but has limited evidence of SGLT2i use in early-phase acute coronary syndrome (ACS). We determined association of early SGLT2i use compared with either non-SGLT2i or dipeptidyl peptidase 4 inhibitor (DPP4i) use in hospitalized patients with ACS.

Methods and results
This retrospective cohort study that used the Japanese nationwide administrative claims database included patients hospitalized with ACS aged ≥20 years between April 2014 and March 2021. The primary outcome was a composite of all-cause mortality or HF/ACS rehospitalization. Using 1:1 propensity score matching, the association with outcomes of the early SGLT2i use (≤14 days after admission) compared with non-SGLT2i or DPP4i use was determined according to the HF treatment. Among 388 185 patients included 115 612 and 272 573 with and without severe HF, respectively. Compared to non-SGLT2i users, the SGLT2i users had a lower hazard ratio (HR) with the primary outcome [HR: 0.83, 95% confidence interval (CI): 0.76–0.91; P &lt; 0.001] in the severe HF group; however, there was no significant difference in the non-severe HF group (HR: 0.92, 95% CI: 0.82–1.03; P = 0.16). SGLT2i use showed a lower risk of the outcome in patients with severe HF and diabetes compared with DPP4i use (HR: 0.83, 95% CI: 0.69–1.00; P = 0.049).

Conclusion
SGLT2i use in patients with early-phase ACS showed a lower risk of primary outcome in patients with severe HF, but the effect was not apparent in patients without severe HF.
Reliability of the novel cleft lip and palate smile esthetic index (CLP-SEI)
Authors: Luyten Jonathan , Thierens Laurent , De Roo Noëmi M C , Temmerman Liesbeth , De Pauw Guy A M 
Published: May 2023

Summary

Background/objectives
The aim of this research was to determine the reliability of a novel index for the assessment of smile esthetics in patients with cleft lip and/or palate (CL ± P) at the end of their multidisciplinary treatment, for use in clinical and academic settings.

Materials and methods
Five orthodontists, five periodontologists, five general practitioners, five dental students, and five laypeople rated the smiles of 10 patients with CL ± P twice at a 2 weeks interval. The rating scale consisted of four main categories: 1. nasolabial esthetics, 2. gingival esthetics, 3. dental esthetics, and 4. overall esthetics. A total of 15 parameters were rated. Intra- and inter-rater agreements were calculated using SPSS.

Results
The inter-rater agreement varied between good and excellent whereby the orthodontists, periodontists, general practitioners, dental students, and laypeople obtained agreement scores of 0.86, 0.92, 0.84, 0.90, and 0.89, respectively. The intra-rater agreement was good with agreement scores of 0.78, 0.84, 0.84, 0.80, and 0.79, respectively.

Limitations
Smile esthetics were rated on static pictures, not in real life or by video recordings in a young adult population.

Conclusions/implications
The cleft lip and palate smile esthetic index is a reliable tool for the assessment of smile esthetics in patients with CL ± P.
Sweetened beverages and incident heart failure
Authors: Zhang Ziteng , Zhang Kun , Sun Ying , Yu Bowei , Tan Xiao , Lu Yingli , Wang Yu , Xia Fangzhen , Wang Ningjian 
Published: May 2023

Abstract

Aims
Recent studies have demonstrated the associations of the consumption of different beverages with cardiometabolic diseases, whereas no studies have investigated such associations in heart failure (HF). Thus, this study aimed to explore the associations of the consumption of sugar-sweetened beverages (SSBs), artificially sweetened beverages (ASBs), and pure fruit/vegetable juices (PJs) with the risk of incident HF.

Methods and results
This prospective cohort study included 209 829 participants in the UK Biobank who completed at least one 24-h diet questionnaire and who were free of baseline HF. Cox proportional hazard models were used to estimate the hazard ratios (HRs) and 95% confidence intervals (CIs). During a median follow-up of 9.9 years, 4328 incident HF cases were recorded. Compared to corresponding non-consumers, individuals who consumed &gt;2 L/week SSBs or ASBs had an increased risk of HF (HR: 1.22, 95% CI: 1.08–1.38 and HR: 1.30, 95% CI: 1.16–1.47, respectively) in the multivariate adjusted model. An inverse association was observed between the consumption of &gt;0–1 L/week PJs and the risk of HF (HR, 0.90; 95% CI, 0.83–0.98). Additionally, a significant interaction was observed between PJ consumption and sleep duration on HF risk (P for interaction = 0.030).

Conclusions
Increased consumption of SSBs or ASBs may be an independent risk factor for HF, whereas moderate intake of PJs may have a protective effect on HF.
Catheter ablation of atrial fibrillation and atrial tachycardia in patients with pulmonary hypertension: a randomized study
Authors: Havranek Stepan , Fingrova Zdenka , Skala Tomas , Reichenbach Adrian , Dusik Milan , Jansa Pavel , Ambroz David , Dytrych Vladimir , Klimes Dalibor , Hutyra Martin , Kautzner Josef , Linhart Ales , Wichterle Dan 
Published: May 2023

Abstract

Aims
Atrial fibrillation (AF), typical atrial flutter (AFL), and other atrial tachycardias (ATs) are common in patients with pulmonary hypertension. Frequently, several supraventricular arrhythmias are successively observed in individual patients. We investigated the hypothesis of whether more extensive radiofrequency catheter ablation of the bi-atrial arrhythmogenic substrate instead of clinical arrhythmia ablation alone results in superior clinical outcomes in patients with pulmonary arterial hypertension (PH) and supraventricular arrhythmias.

Methods and results
Patients with combined post- and pre-capillary or isolated pre-capillary PH and supraventricular arrhythmia indicated to catheter ablation were enrolled in three centres and randomized 1:1 into two parallel treatment arms. Patients underwent either clinical arrhythmia ablation only (Limited ablation group) or clinical arrhythmia plus substrate-based ablation (Extended ablation group). The primary endpoint was arrhythmia recurrence &gt;30 s without antiarrhythmic drugs after the 3-month blanking period. A total of 77 patients (mean age 67 ± 10 years; 41 males) were enrolled. The presumable clinical arrhythmia was AF in 38 and AT in 36 patients, including typical AFL in 23 patients. During the median follow-up period of 13 (interquartile range: 12; 19) months, the primary endpoint occurred in 15 patients (42%) vs. 17 patients (45%) in the Extended vs. Limited ablation group (hazard ratio: 0.97, 95% confidence interval: 0.49–2.0). There was no excess of procedural complications and clinical follow-up events including an all-cause death in the Extended ablation group.

Conclusion
Extensive ablation, compared with a limited approach, was not beneficial in terms of arrhythmia recurrence in patients with AF/AT and PH.

Clinical Trials Registration
ClinicalTrials.gov; NCT04053361.
Explaining the gender gap in COVID-19 vaccination attitudes
Authors: Toshkov Dimiter 
Published: May 2023

Abstract

Background
Women have been significantly more likely than men to express hesitancy toward COVID-19 vaccination and, to a lesser extent, to refuse vaccination altogether. This gender gap is puzzling because women have been more likely to perceive higher risks from COVID-19, to approve more restrictive measures to fight the pandemic and to be more compliant with such measures.

Methods
This article studies the gender gap in COVID-19 vaccination attitudes using two nationally representative surveys of public opinion fielded in February 2021 and May 2021 in 27 European countries. The data are analyzed using generalized additive models and multivariate logistic regression.

Results
The data analyses show that hypotheses about (i) pregnancy, fertility and breastfeeding concerns, (ii) higher trust in Internet and social networks as sources of medical information, (iii) lower trust in health authorities and (iv) lower perceived risks of getting infected with COVID-19 cannot account for the gender gap in vaccine hesitancy. One explanation that receives support from the data is that women are more likely to believe that COVID-19 vaccines are unsafe and ineffective and this leads them to perceive the net benefits of vaccination as lower than the associated risks.

Conclusions
The gender gap in COVID-19 vaccine hesitancy results to a large extent from women perceiving higher risks than benefits of the vaccines. While accounting for this and other factors decreases the gap in vaccine hesitancy, it does not eliminate it completely, which suggests further research is needed.
Using the AKAR3-EV biosensor to assess Sch9p- and PKA-signalling in budding yeast
Authors: Botman Dennis , Kanagasabapathi Sineka , Savakis Philipp , Teusink Bas 
Published: May 2023

AbstractBudding yeast uses the TORC1-Sch9p and cAMP-PKA signalling pathways to regulate adaptations to changing nutrient environments. Dynamic and single-cell measurements of the activity of these cascades will improve our understanding of the cellular adaptation of yeast. Here, we employed the AKAR3-EV biosensor developed for mammalian cells to measure the cellular phosphorylation status determined by Sch9p and PKA activity in budding yeast. Using various mutant strains and inhibitors, we show that AKAR3-EV measures the Sch9p- and PKA-dependent phosphorylation status in intact yeast cells. At the single-cell level, we found that the phosphorylation responses are homogenous for glucose, sucrose, and fructose, but heterogeneous for mannose. Cells that start to grow after a transition to mannose correspond to higher normalized Förster resonance energy transfer (FRET) levels, in line with the involvement of Sch9p and PKA pathways to stimulate growth-related processes. The Sch9p and PKA pathways have a relatively high affinity for glucose (K0.5 of 0.24 mM) under glucose-derepressed conditions. Lastly, steady-state FRET levels of AKAR3-EV seem to be independent of growth rates, suggesting that Sch9p- and PKA-dependent phosphorylation activities are transient responses to nutrient transitions. We believe that the AKAR3-EV sensor is an excellent addition to the biosensor arsenal for illuminating cellular adaptation in single yeast cells.
Craft, Labour and Cabinets of Curiosities: Rethinking the Body of the Artisan
Authors: Rublack Ulinka 
Published: May 2023

Abstract
This article asks how we should best historically situate processes of making and knowing in early modern luxury crafts. It focuses on the Augsburg merchant Philipp Hainhofer (1578–1647) and his celebrated cabinets of curiosities. The article methodologically argues for the need to cross-fertilize scholarship on ‘the body of the artisan’ (Pamela H. Smith) with the history of labour. The notion of the European ‘artisan’ involved in ‘making and knowing’ has unwittingly tended to conjure up the image of an unquestionably male and often autonomous practitioner soberly immersed in experiment. By contrast, the article argues that lived ‘bodies’ involved in making and knowing were diverse and subjected to far more disciplining and strain than has hitherto been highlighted. A culture of secrecy fostered isolation. Increased alcohol consumption interrelated with the cultivation of wit as intellectual and affective practice. The article considers how differences in social status and religious beliefs created tensions among these makers and pays attention to the gendered nature of these types of employment and the hidden global types of knowledge and labour upon which they depended. By focusing primarily on Hainhofer’s published and unpublished correspondence, the article argues against the generalized conception that artefacts emerged from a ‘flow’ between makers and materials and demonstrates how early modern craft labour can be situated in social, economic and cultural contexts. Material communities in the luxury crafts were characterized by systemic strain, conflicts and the need to find shortcuts, but also by pleasure, wit, resistance, tenacity and opportunities for conceptual thinking.