bluuebunny/crossref_metadata_2025_split huggingface dataset:
Identification and validation of a prognostic signature based on six immune-related genes for colorectal cancer
Authors: Zheng Lifeng , Xu Ziyu , Zhang Wulou , Zhou Shu , lin Hao , Zhang Yepeng , Liu Zonghang , Gu Xi 
Published: September 2023

Abstract

Background
Colorectal cancer (CRC) is a prevalent malignancy with high death and morbidity rates. Even though the significant efficacy of immunotherapy is well-established, it is only beneficial for a limited number of individuals with CRC.
Methods
Differentially expressed immune-related genes (DE-IRGs) were retrieved from The Cancer Genome Atlas (TCGA), Gene Expression Omnibus (GEO), and Immport databases. The prognostic signature comprising DE-IRGs was developed by means of Univariate, Lasso, and Multivariate Cox-regression analyses. Following this, a nomogram integrating the independent prognostic factors was also developed. CIBERSORT was applied to ascertain the immune cell infiltration (ICI). Furthermore, wound healing, colony formation, migration, and invasion assays were executed to study the involvement of ACTG1 in CRC.
Results
A signature including six DE-IRGs was developed. It could estimate the rate of overall survival (OS) accurately for the TCGA and GSE38832 cohorts. The risk score (RS) of the signature was an independent factor for OS. Moreover, a nomogram encompassing age, RS, and pathological T could accurately predict the long-term OS probability of individuals with CRC. The high-risk group had an elevated proportion of ICI relative to the low-risk group, including native B cells. Additionally, ACTG1 expression was upregulated, which supported the proliferation, migration, and invasion abilities of CRC cells.
Conclusions
An immune-related prognostic signature was developed for predicting OS and to ascertain the immune status in individuals with CRC. It could provide new insights into accurate immunotherapy for individuals with CRC. Moreover, ACTG1 can possibly serve as a new immune biomarker.
The risk of urinary incontinence among women with obese in China： a meta-analysis
Authors: Shang Xin , Zhao Mingzhu , Fu Yu , Guo Panpan , Jin Xiaoqin , Wang Chenxiao , Wang Ping , Wang Ying , Yan Shuxun 
Published: September 2023

Abstract
Objective
The objective of this meta-analysis is to assess the potential association between obesity and overweight, and the susceptibility to Urinary Incontinence (UI) among Chinese women.
Methods
We searched PubMed, Cochrane Library, Embase, China National Knowledge Infrastructure (CNKI) and WANFANG Database to obtain observational study published between the establishment of the database and 10 July 2023. We used the Newcastle Ottawa Scale (NOS) and the Quality Assessment Program of the American Institute for Healthcare Quality and Research (AHRQ) to evaluate the quality of the study. When P > 0.1 and I2 ≤ 50%, a fixed effect model is used. Otherwise, a random effects model is applied. Funnel plots and Egger's test were used to explore publication bias. All statistical analyses were conducted in Stata 14.0.
Results
This meta-analysis comprises 13 observational studies involving a total of 76,606 individuals. The pooled analysis reveals no statistically significant association between overweight and the risk of UI (odds ratio [OR] = 1.23; 95% confidence interval [CI]: 0.97–1.56; I2 = 94.6%, P = 0.000). Among Chinese women, obesity significantly increases the likelihood of developing urinary incontinence (OR = 2.00; 95% CI: 1.55–2.58; I2 = 88.8%, p = 0.000). Subgroup analysis demonstrates no significant association between obesity and mixed urinary incontinence (MUI) among obese women in China (OR = 1.31; 95% CI: 0.98–1.75; I2 = 0.0%, P = 0.806). Nevertheless, obesity is significantly associated with stress urinary incontinence (SUI) (OR = 1.72; 95% CI: 1.47–2.01; I2 = 34.3%, P = 0.201). Subgroup analysis of regional types shows that obesity in southern and northern China, eastern, central and western regions is associated with a high risk of UI.
Conclusions
Obesity is found to be positively associated with an elevated likelihood of UI in Chinese women. This study can provide basis for the prevention and treatment of UI, and provide better prevention and management for alleviating the symptoms of UI in Chinese adult women.
Synthesis, Characterization, and Evaluation of dipotassium dodecyl diphenyl ether disulfonate as anionic surfactant inside in synthesis the Emulsion polymer Lattices
Authors: Khattab Asaad Faisal , Ismael Yonis Ahmed , Habab Ahmed Taher El 
Published: September 2023

Abstract
The presence of anionic surfactant in mixture with a non-ionic surfactant usually results in an increase in the cloud point of the non-ionic component. Indicating that the ionic component is forming mixed micelles with the non-ionic surfactant. In this study the anionic surfactant is dipotassium dodecyl diphenyl ether disulfonate designated as (DPDDPEDS), was synthesized. The structure was characterized by using spectroscopic measurements. In addition, its surface properties and foaming properties were investigated. The results indicated that DPDDPEDS exhibits excellent surface activity. It was found that the DPDDPEDS as anionic surfactant have low krafft point approximation 10 ºC.
Bioinformatics analysis of GPCRs: A window into breast tumorigenesis and metastasis
Authors: Khan Safir Ullah , Hernández-González Karla Daniela , Murillo-González Fatima Elizabeth , Zaheer Saima , Méndez-Tenorio Alfonso , Luna-Arias Juan Pedro 
Published: September 2023

Abstract
G-protein-coupled receptors (GPCRs) are known signal transducers and are increasingly implicated in tumorigenesis, but their precise roles in breast cancer progression remain underexplored. We scrutinized the mRNA expression profiles of fifty GPCRs in breast tumor tissue compared to normal tissue. Prominently, PTGFR, VIPR1, ADGRL2/3, LPAR1, and S1PR1 were found to be downregulated in breast cancer tissues, while ADGRL2 and CELSR1 exhibited upregulation. LPAR1, TSHR, and S1PR2/4 expression significantly influenced HPV and lncRNA entry in breast cancer compared to normal counterparts. We also explored correlations between GPCRs and various clinicopathological attributes, encompassing menopausal age, receptor status, nodal status, P53 status, triple-negative breast cancer (TNBC), Scarf–Bloom–Richardson grade (SBR), and nottingham prognostic index (NPI). Survival analysis unveiled thirty-three GPCRs that significantly influenced prognosis, with a high net alteration frequency of 84.41% across all 50 GPCRs. Our investigation unraveled complex co-expression associations between GPCRs and genes involved in pivotal signaling pathways, growth factor receptors, PKCs, GEFs, and markers indicative of metastasis and proliferation. Moreover, our study identified potential interactions between rutin, cucurbitacins, ellagic acid, and tilliroside with the targeted GPCRs. P2RY8, LPAR3, S1PR5, LPAR2, CELSR3, and GRM8 may function as oncogenes whereas, ADGRL2, LPAR1/4/6, and GRM6/7, PTGFR, TSHR, SMO, CCKBR, S1PR2/4 and CHRM1/3 may be tumor-suppressors for breast cancer.
Control of antibody orientation on graphene using porphyrin linker molecules for high-performance graphene-based immuno-biosensors
Authors: Yin Tianyi , Ramadan Sami , Xu Xi , Huang Zhiping , Rosa Bruno Gil , Gaboriau David , Merali Nabeel , Keshavarz Meysam , Mustafa Ahmad Bin Muhammad , Song Jiapeng , Alodan Sarah , Chen Michael , Hammer Matthew , Petrov Peter , Frampton Adam , Skinner Stephen , Alford Neil , Klein Norbert 
Published: September 2023

Abstract
The appropriate orientation of antibodies on the graphene surface is critical for the high performance of graphene biosensors. In this paper, we show that a predominantly vertical orientation of antibodies to the graphene surface can be achieved by functionalizing the surface with tetrakis (4-carboxyphenyl) porphyrin (TCPP). We used this method to detect exosome biomarkers using a graphene field effect transistor (GFET) platform, and achieved a two orders of magnitude improvement in the detection limit of a GFET biosensor compared with that achieved by conventionally used 1-pyrene butanoic acid succinimidyl ester (PBASE). Through experimental analysis and computational simulation, we revealed that the antibody can highly exhibit vertical orientation on TCPP-functionalized graphene, while mixed orientations on PBASE-functionalized graphene. We further validated the high efficiency of TCPP method by detecting pancreatic cancer exosome biomarkers in clinical samples. This biofunctionalization method could enable the ultrasensitive detection of disease biomarkers on a graphene surface.
Multimodal system for recording individual-level behaviors in songbird groups
Authors: Rüttimann Linus , Rychen Jörg , Tomka Tomas , Hörster Heiko , Rocha Mariana D. , Hahnloser Richard H.R. 
Published: September 2023

Abstract
In longitudinal observations of animal groups, the goal is to identify individuals and to reliably detect their interactive behaviors, including their vocalizations. However, to reliably extract individual vocalizations from their mixtures and other environmental sounds remains a serious challenge. Promising approaches are multimodal systems that exploit signal redundancy and make use of animal-borne wireless sensors. In this vein, we designed a modular recording system (BirdPark) that yields synchronized data streams. We recorded groups of songbirds with multiple cameras and microphones and recorded their body vibrations with custom low-power frequency-modulated (FM) radio transmitters. We developed a custom software-defined radio receiver with a multi-antenna demodulation technique that increased the signal-to-noise ratio of the received radio signals by 6.5 dB and reduced the signal loss rate due to fading by a factor of 63 to only 0.01% of the recording time compared to single-antenna demodulation. Nevertheless, neither a single vibration sensor nor a single microphone is sufficient by itself to detect the complete vocal output of an individual. Even in the minimal setting of an animal pair, an average of about 3.7% of vocalizations remain undetected within each sensor modality. Our work emphasizes the need for high-quality recording systems and for multimodal analysis of social behavior.
Uptake, effectiveness and safety of COVID-19 vaccines in the immunocompromised population: A population-based cohort study in England
Authors: Chen Daniel , Copland Emma , Hirst Jennifer , Mi Emma , Dixon Sharon , Coupland Carol , Hippisley-Cox Julia 
Published: September 2023

Abstract
Immunocompromised individuals face increased risks of severe COVID-19 outcomes, underscoring the importance of receiving COVID-19 vaccination. However, there's a lack of comprehensive real-world data on their COVID-19 vaccine uptake, effectiveness, and safety profile. We analysed data in the QResearch UK database from 01/12/2020 to 11/04/2022. We included 12,274,948 people aged ≥ 12 years in our analysis, of whom 583,541 (4.8%) were immunocompromised, defined as on immune-modifying drugs, chemotherapy, organ transplants, or dialysis. Overall, 93.7% of immunocompromised patients received at least one COVID-19 vaccine dose. Uptake reduced with increasing deprivation (Hazard Ratio [HR] 0.78 [95% CI 0.77–0.79] in the most deprived quintile compared to the least for the first dose). Using a nested case-control design, estimated vaccine effectiveness against COVID-19 hospitalisation 2–6 weeks after the second and third doses compared to the unvaccinated was 78% (95%CI 72–83) and 91% (95%CI 88–93) for the immunocompromised, versus 85% (95%CI 83–86) and 86% (95%CI 85–89) respectively for the general population. COVID-19 vaccines were protective against intensive care unit admission and death in both groups. There were no differing risks of adverse events during the first 28 days after each dose between the two populations. These findings underscore the importance of ongoing vaccination prioritisation for immunocompromised individuals to maximise protection against severe COVID-19-related outcomes.
Disturbance feedforward compensation based on Modelica modeling with the Self-immunity study of a quadrotor UAV
Authors: ZHOU HAO , BAO XIAOPENG , LIANG QIAO , ZHANG HONGGANG 
Published: September 2023

Abstract
Due to the nonlinearity, underactuated, strong coupling, multi-data flow direction, and vulnerability to internal and external disturbances, the widely used PID control technology has been very difficult. Some emerging nonlinear control theory algorithms have good simulation performance, but they rely heavily on exact mathematical models, require a large amount of computation, and take a long time to solve. Therefore, based on the advantages of non-causal declarative modeling in Modelica language, this paper focuses on the analysis and implementation of the mechanism of observation, estimation, and compensation of nonlinear ADRC disturbances, PID-NLADRC linear nonlinear combined control strategy is designed to realize the flight control of a four-rotor UAV. The multi-target applications, such as fixed-point hover, cone spiral trajectory tracking and air obstacle avoidance fillet moment trajectory tracking, are verified respectively. The simulation results show that the designed PID-NLADRC control strategy has better performance than the Cascade PID control strategy in suppressing the fixed-point hover disturbance, it is fully verified that the PID-NLADRC control strategy has a smaller Euclidean range deviation and stronger disturbance rejection ability for tracking trajectories with different properties such as plane or space, smooth or transient, it can better meet the requirements of fast, accurate and robust flight control of four-rotor UAV.
A High Performance Random Forest Machine Learning Algorithm for QoS- Based Web Services Composition
Authors: Choudhury Ziaul Haque 
Published: September 2023

AbstractService-based architecture becomes a key software interface for complex applications which can be dynamically and flexibly compounded by integrating existing Web services with standard protocols from various providers. New web services can adversely affect the quality of service and customer satisfaction through their rapid introduction into a competitive business environment. Hence, it remains a constant problem in state of the art research how to collect, aggregate and employ data on individual quality of service (QoS) components to obtain the optimum quality of service of a composite service that responds to customer needs. This study proposes a Random Forest (RF) Algorithm for high-performance machine learning web services composition (WSC) problem. In this experiment, the validation is done using value iteration, iterative policy evaluation, and policy iteration algorithm. In this experiment, we illustrate WSC difficulties how to solve and demanding an entire sequence of 1000,000 Web services can be measured using an Intel Core i7 device with a 32GB RAM, requiring the selection of 10,000 services from the current system and less than 130 seconds. Besides, only seven individual web services pose a real WSC problem that needs processing power of less than 0.03 seconds.
Analysis of soil fertility and heavy metal characteristics in open-pit mining areas in northern Shaanxi
Authors: Wang Na , Liu Zhe , Sun Yingying , Lu Nan , Luo Yuhu 
Published: September 2023

Abstract
The northern Shaanxi region stands as the primary coal production hub in Shaanxi Province. Assessing the risk of heavy metal contamination in this mining area is of paramount importance for ensuring the sustainable development of the regional ecosystem and safeguarding human health. This study specifically focused on the Hongliulin mining area, where a total of 40 soil samples were meticulously collected and analyzed from within a 1000-meter radius extending from the tailings dam. The findings revealed that soil pH within the 0-1000m range generally leaned towards the alkaline side. In terms of soil nutrient content, encompassing factors such as soil organic matter (SOM), total nitrogen (TN), total phosphorus (TP), total potassium (TK), alkali nitrogen (AK), available phosphorus (AP), and quick-acting potassium (AK), the variations fell within the following ranges: 2.23~13.58 g/kg, 0.12 ~ 0.73 g/kg, 0.18 ~1.15 g/kg, 9.54 ~35.82 g/kg, 2.89 ~ 6.76 mg/kg, 3.45~11.25 mg/kg, and 5.86~130.85 mg/kg. Collectively, these values indicate relatively low levels of soil nutrients. Within the 0-500m range of soil samples, the average concentrations of Cd, Hg, Pb, and As were 0.778, 0.198, 24.87, and 17.92 mg/kg, respectively. These concentrations exceeded the established soil background values of Shaanxi Province and emerged as the primary pollutants in the study area. Within this same range, the mean values of eight heavy metals (Pi) were ranked in the following descending order: 1.726 (Hg), 1.400 (As), 1.129 (Cr), 1.109 (Pb), 0.623 (Zn), 0.536 (Cd), 0.309 (Cu), and 0.289 (Ni). With the exception of Hg, As, Cr, and Pb, which exhibited slight pollution, the other heavy metals were found to be within acceptable pollution limits for this sampling range, in line with the results obtained using the geo-accumulation index method. The average potential ecological risk index (RI) for the eight heavy metals in the study area stood at 185.0, indicating a moderate overall pollution level. When assessing individual elements, the proportions of ecological risk attributed to Hg, As, Pb, and Cd were 34.57%, 27.44%, 25.11%, and 23.11%, respectively. This suggests that the primary potential ecological risk elements in the study area are Hg and As, followed by Cd and Pb. Notably, heavy metals Hg and Pb, as well as As and Pb, exhibited significant positive correlations within the sampling area, suggesting a common source. An analysis of the relationship between soil physicochemical properties and heavy metals indicated that soil pH, organic matter, nitrogen, and phosphorus were closely linked to heavy metal concentrations. The soil in the sampling area has undergone significant disturbances due to human activities, resulting in heavy metal pollution and nutrient deficiencies.
A unique sigma/anti-sigma system involved in dormancy and revival of bacterial sporangiospores
Authors: Ohnishi Yasuo , Tezuka Takeaki , Mitsuyama Kyota , Date Risa 
Published: September 2023

Abstract
Bacterial spores convert from dormant to active cells upon exposure to environmental stimuli; however, their molecular mechanisms remain unclear. Here, we report a unique sigma/anti-sigma system involved in the dormancy and revival of sporangiospores in the filamentous bacterium Actinoplanes missouriensis. We propose that the sigma factor σSsdA activates transcription of a subset of genes for the maintenance of dormancy, including the oxidative stress response, directly or indirectly in sporangiospores. The cognate anti-sigma factor SipA does not seem to be a switch for spore revival; rather, it seems to modulate σSsdA function to achieve appropriate transcription levels of σSsdA-dependent genes during and/or after sporangium formation. Both SipA and σSsdA possess an anti-sigma factor antagonist domain, thereby enabling interactions between the anti-sigma factor domain of SipA and the anti-sigma factor antagonist domain of σSsdA. We also identified a two-component regulatory system (RsdK-RsdR) indirectly activated by σSsdA as a key factor that represses revival of spores, more specifically, initiation of sporangium dehiscence. Thus, RsdK-RsdR plays a pivotal role in a molecular mechanism that delays the initiation of awakening of sporangiospores. This study reveals a previously unknown aspect of the general biological phenomena of cell dormancy and awakening.
LDCT Screening Results among Health Check-ups Population
Authors: Bao Ting , Liu Bingqing , Li Ruicen , Li Zhenzhen , Ji Guiyi , Wang Youjuan , Yang Hanwei , Li Weimin , Huang Wenxia , Huang Yan , Tang Huairong 
Published: September 2023

Abstract
Objective
To compare the LDCT screening results between eligible and ineligible screening candidates in preventive health check-ups population.
Study Design and Setting
Using a real-world LDCT screening results among people who took yearly health check-up in health management center of West China Hospital between 2006 and 2017. Objects were classified according to the China National Lung Cancer Screening Guideline with Low-dose Computed Tomography (2018 version) eligibility criteria. Descriptive analysis were performed between eligible and ineligible screening candidates.
Results
The proportion of ineligible screening candidates was 64.13% (10259), and among them there were 4005(39.04%) subjects with positive screenings, 80 cases had a surgical lung biopsy. Pathology results from lung biopsy revealed 69 cancers (true-positive) and 11 benign results (false-positive). The difference of the false-positive biopsy rate between ineligible screening candidates (13.75%) and eligible screening candidates(15.00%) was no statistically significant (P>0.05). Further, in ineligible screening candidates, the proportion of current smokers was higher among males compared to females (53.85% vs. 4.88%, P<0.05). Of the 69 lung cancer patients detected in ineligible screening candidates, lung adenocarcinoma accounts for a high proportion of lung cancers both in male (75.00%) and female (85.00%).
Conclusion
The proportion of ineligible screening candidates was high in health check-ups population.
Effect of Thermocycling on Acetal Resin vs PEEK Surface Hardness and Flexure Strength of Implant-Retained Overdenture Bars. In Vitro Study
Authors: Salem Sherihan , AlSourori Ali Abdulghani , Mostafa Marwa Hassan 
Published: September 2023

Abstract
Background: Implant-supported mandibular overdentures with bar attachments are considered an excellent option for a completely edentulous mandible. It provides a long-lasting and reliable solution to restore function and aesthetics to the patient. These enhancements are related to a reduced rate of bone ridge resorption, improved retention, and support for the prosthesis, all of which lead to better mastication, function, and overall health. Many materials can be used for the construction of bar attachments, among which are peek and acetal resin materials. The choice between Peek and acetal bar attachments eventually depends on the application, considering factors such as load-bearing requirements, biocompatibility, adaptability, and ease of use.
Methods: Twelve 3D printed edentulous mandible model each of them received two implants .Models was then divided into two equal groups. Group1: PEEK bars were fabricated by thermo pressed technique while Group 2: Acetal resin bars were fabricated by thermo pressed technique. Surface hardness and flexure strength were then evaluated and statistically analyzed before and after thermocycling.
Results: PEEK Group revealed significant higher surface hardness than Acetal resin before and after thermocycling. Regarding flexure strength PEEK showed insignificant increase than Acetal before thermocycling, however the PEEK group displayed much higher values than the Acetal group following the thermocycling, resulting in a significant difference between the two groups.
Conclusions: Bar made of PEEK showed more promising surface hardness and flexure strength than Acetal resin bar