bluuebunny/crossref_metadata_2025_split huggingface dataset:
A195 DURABILITY OF SEROLOGICAL RESPONSES AFTER SECOND, THIRD AND FOURTH DOSE OF SARS-COV-2 VACCINATION IN INFLAMMATORY BOWEL DISEASE: A PROSPECTIVE COHORT STUDY
Authors: Sharifi N , Ma C , Seow C , Quan J , Hracs L , Caplan L , Markovinović A , Herauf M , Windsor J , Coward S , Buie M , Gorospe J , Panaccione R , Kaplan G 
Published: March 2023

Abstract

Background
Adequate serological responses following two-dose regimens and additional doses of SARS-CoV-2 vaccination have been demonstrated for the vast majority of those with IBD. However, antibody levels following 2nd, 3rd, and 4th dose SARS-CoV-2 vaccination may decrease over time in the IBD population.

Purpose
We assessed the durability of serological responses to 2nd, 3rd, and 4th dose SARS-CoV-2 vaccination over time in a cohort of IBD patients.

Method
Adults with IBD who received at least one dose of a SARS-CoV-2 vaccine (n=559) were evaluated for serological response to the spike protein of SARS-CoV-2 using the Abbott IgG II Quant assay with a seroconversion threshold of ≥ 50 AU/mL. The geometric mean titer (GMT) with 95% confidence intervals (CI) were calculated and stratified by weeks (1–8, 8–16, 16–24, 24+ weeks) after each vaccine dose. We compared stratified GMTs with Mann–Whitney U tests using a significance level of 0.05.

Result(s)
Our cohort (n=559) comprised the following patient characteristics: 82.8% were 18–65 years-old (n = 463), 53.1% were female (n =297), and 71.6% had Crohn’s disease (n =400). IBD medications were classified in the following mutually exclusive groups: No immunosuppressives 10.5% (n = 59), anti-TNF monotherapy 35.8% (n = 200), immunomodulatory monotherapy 2.1% (n =12 ), vedolizumab 11.8% (n =66 ), ustekinumab 20.4% (n =114 ), tofacitinib 1.2% (n =7 ), combination therapy 15.9% (n = 89), and prednisone 2.1% (n =12). For vaccine type, 85.6% and 82.3% had Pfizer for 3rd and 4th dose, respectively, while the remainder had Moderna. Seroconversion rates 1–8 weeks after 3rd and 4th dose were both 99.9%.
Figure 1 compares GMTs with 95% CI by weeks after each vaccine dose. GMTs are highest 1–8 weeks after 2nd dose (4053 AU/mL; 95% CI: 3468, 4737 AU/mL; n=337), 3rd dose (12116 AU/mL; 10413, 14098 AU/mL; n=256), and 4th dose (14337 AU/mL; 10429, 19710 AU/mL; n=67). Subsequently, antibody levels decay from 1–8 weeks to 8–16 weeks (p&lt;0.001) for 2nd dose (mean difference: –2224 AU/mL), 3rd dose (mean difference: –7526 AU/mL), and 4th dose (mean difference: –9715 AU/mL). Compared to 16–24 weeks after 2nd dose, antibody levels 24+ weeks after were similar (GMTs: 795 AU/mL vs. 1043 AU/mL, p=0.52). For third dose, antibody levels 8–16 weeks and 16–24 weeks after vaccination were similar (4590 AU/mL vs. 4073 AU/mL, p=0.73) along with 16–24 weeks compared to 24+ weeks after vaccination (4073 AU/mL vs. 5876 AU/mL, p=0.18).

Image

Conclusion(s)
Within 1–8 weeks after each dose of vaccine, serological responses spikes with each subsequent dose yielding a higher GMT. While antibody levels decay 8–16 weeks after each dose, similar GMT levels beyond 16 weeks may indicate durability of antibody levels over a longer duration of time.

Disclosure of Interest
None Declared
A217 A REVIEW OF MOBILE HEALTH APPLICATIONS FOR INDIVIDUALS LIVING WITH INFLAMMATORY BOWEL DISEASE USING MOBILE APPLICATION RATING SCALE (MARS)
Authors: Jannati N , Salehinejad S , Kuenzig E , Peña-Sánchez J N 
Published: March 2023

Abstract

Background
Inflammatory bowel disease (IBD) is a chronic disorder that can be managed but not cured. The relapsing and remitting symptoms of IBD impact patients' quality of life and is associated with considerable healthcare costs. Different mobile health apps have been developed around the world for IBD and other chronic medical conditions. These apps can potentially decrease healthcare utilization and the burden of the disease.

Purpose
We aimed to review IBD mobile health apps available in English and evaluate their quality and content.

Method
We searched the Apple Store and Play Store in July 2022 using IBD-related keywords (e.g., “IBD,” “Crohn,” “Crohns,” “colitis,” “ulcerative colitis,” and “inflammatory bowel disease.”) to identify apps for iOS and Android devices, respectively. We included apps available in English, designed specifically for IBD, and free to download and use. Two researchers reviewed, rated, and evaluated the retrieved apps independently. The Mobile App Rating Scale (MARS) was used to rate the included apps. The MARS rates apps on a scale of 1 to 5 and includes an overall score as well as scores for engagement, functionality, information, aesthetics, and subject quality. A score ≥3 is deemed acceptable.

Result(s)
Search queries yielded 88 relevant apps, of which 38 met the inclusion criteria and were included in this review. The included apps for IBD were most tracking and monitoring symptoms of the disease (n=22). Two of the apps were affiliated with universities, and three of the apps were specifically developed for children. In addition, 11 apps were designed for IBD, regardless of disease subtype (Crohn’s disease [CD] or ulcerative colitis [UC]), and 27 apps were designed specifically for either UC or CD. The mean MARS score for IBD apps was 3.3 (SD=0.6), with more than half deemed acceptable. Apps scored highest on the functionality (mean=3.7, SD=0.6) and information (mean=3.6, SD=0.5) dimension of MARS and lowest on the engagement dimension (mean=3, SD=0.8).

Image

Conclusion(s)
Most IBD-related apps provide acceptable information and functionality but should improve their engagement to be welcomed by users. This review provides a roadmap for improving available apps and future app development for individuals with IBD. App developers in the field of IBD must ensure they create high-quality, engaging, esthetic and evidence-based apps.

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None

Disclosure of Interest
None Declared
A229 THE BACTERIAL VIRULENCE FACTOR NLEA UNDERGOES HOST-MEDIATED O-LINKED GLYCOSYLATION
Authors: Burns L , Giannakopoulou N , Zhu L , Xu Y Z , Khan R H , Bekal S , Schurr E , Schmeing T M , Gruenheid S 
Published: March 2023

Abstract
NOT PUBLISHED AT AUTHOR’S REQUEST

Disclosure of Interest
None Declared
A259 PEDIATRIC ACUTE LIVER FAILURE: A RETROSPECTIVE CROSS-SECTIONAL STUDY FROM A TERTIARY CARE CENTER IN JORDAN
Authors: Altamimi E , Al Sharie A , Al Zu’bi Y , Mahadin R , Mahadin R , Al-Faqih S , Abu-Ekteish F 
Published: March 2023

Abstract

Background
Pediatric acute liver failure (PALF) is a rare, rapidly progressing and life threating condition associated with high mortality rate despite optimal medical management. The clinicopathological characteristics of PALF could harbor new emerging avenues in patients’ stratification and management. Although, PALF is being studied extensively, no data from a Mediterranean cohort were elucidated in the medical literature

Purpose
To analyse the clinical presentation, aetiology, and outcome of children presenting with acute liver failure, admitted to a tertiary hospital (King Abdullah University Hospital, Irbid, Jordan).

Method
The electronic medical records at our center were retroactively accessed to identify PALF patients. Records of all children with acute liver failure over a 5-year period were reviewed retrospectively. Data on age, sex, clinical presentation, underlying cause, medical management, complication development and outcome were collected.

Result(s)
A total of 86 patients were identified, the mean age is 6.64 ± 5.82 years with male predominance (n = 46, 56.98%). The majority of PALF cases were not labeled with a definitive clinical diagnosis (n = 41, 47.67%). PALF etiologies include infections (n = 15, 17.44%), trauma (n = 10, 11.63%), malignancy-related (n = 9, 10.47%), genetic/metabolic (n = 7, 8.14%), and congenital anomalies (n = 4, 4.65%). The overall mortality rate is 43.02% and the average length of stay is 20.67 days. The majority of patients were managed in intensive care units (n = 67, 77.91%) and 20.93% (n = 18) had a positive blood culture during their hospitalization period.

Conclusion(s)
A more robust diagnostic algorithms should be implemented in PALF cases. The establishment of a country-based liver transplantation program is highly endorsed besides the implementation of many diagnostic tools to undercover the indeterminate causes of PALF.

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None

Disclosure of Interest
None Declared
A269 THE MODULATION OF INTESTINAL INFLAMMATION BY PARABACTEROIDES GOLDSTEINII IN MURINE MODELS OF COLITIS
Authors: Gerkins C , Oliero M , Hajjar R , Vennin Rendos H , Fragoso G , Calvé A , Diop K , Routy B , Santos M 
Published: March 2023

Abstract

Background
Inflammatory bowel diseases (IBDs), comprised mainly of Crohn’s disease and ulcerative colitis, are characterized by chronic and relapsing intestinal inflammation. Though the etiology of IBDs is not entirely understood, it is thought that an imbalance of the gut microbiota is one contributing factor, making it a potential target for the treatment of IBDs.

Purpose
This study aims to examine how the potential probiotic Parabacteroides goldsteinii modulates intestinal inflammation in the context of experimental colitis.

Method
Wild type C57BL/6 mice were divided into three groups. One group was given phosphate-buffered saline (PBS) via oral gavage without any further treatment. The second group was treated with 1% dextran sodium sulfate (DSS) for twelve days induce colitis and given PBS every three days. Finally, the third group was also treated with 1% DSS for twelve days and given P. goldsteinii via oral gavage every three days. Weight loss, stool consistency, and intestinal bleeding were monitored and were used to calculate the Disease Activity Index (DAI) daily. Postmortem, colon length was measured, and inflammation score was evaluated on hematoxylin and eosin-stained samples of the colon and spleen. Finally, interleukin (IL)-6 levels were measured in the colon tissue and the serum by ELISA.

Result(s)
Colonization was confirmed at day three after gavage by real-time PCR. Preliminary results show that DSS-treated mice lost significantly more weight by day twelve than mice colonized with P. goldsteinii. Additionally, on days eight and twelve, mice colonized with P. goldsteinii had significantly lower DAI scores than mice that received only PBS. Finally, mice colonized with P. goldsteinii had significantly longer colons than those that received only PBS.

Conclusion(s)
The preliminary data indicates that mice colonized with P. goldsteinii had less severe symptoms of colitis than mice only given PBS. This suggests that P. goldsteinii may attenuate inflammation in mice treated with DSS.

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CIHR, Other

Please indicate your source of funding;
ICM

Disclosure of Interest
None Declared
A272 EVIDENCE OF SEX DIFFERENCES IMPACTING PAIN SIGNALING BY LUMINAL MEDIATORS IN IRRITABLE BOWEL SYNDROME
Authors: Ables S , Bennett A , Vanner S , Lomax A , Reed D 
Published: March 2023

Abstract

Background
Irritable bowel syndrome (IBS) is more than twice as common in women and female patients report more severe abdominal pain. This suggests that sex-specific mechanisms may contribute to the pathophysiology of IBS. Many IBS patients have altered gut microbiota and luminal meditators, implicating the gut microbiota in abdominal pain. These luminal mediators can alter excitability of visceral nociceptors, thus potentially contributing to abdominal pain in IBS. Furthermore, in a subset of IBS patients a low FODMAP diet (LFD) reduces the effect of luminal mediators on pain sensing neurons. The LFD may improve abdominal pain at greater rates in women, and numerous putative mechanisms contributing to abdominal pain in IBS are susceptible to sex-specific mediators. However, it is unknown whether luminal mediators have similar effects on visceral pain signaling in both males and females. We hypothesize that luminal mediators will cause greater differences in neuronal excitability and pain signaling in female mice due to sex-specific factors.

Purpose
To determine whether FS from IBS patients (IBS FS) affects nociceptors from female mice more than nociceptors from male mice.

Method
Neurons from dorsal root ganglia from male and female mice were incubated overnight in media containing fecal supernatant (FS) from IBS patients (N=2 females) before and after the LFD, or healthy controls (HC, N=1 female and 1 male). Ratiometric Ca2+ imaging with FURA-2-AM was employed to quantify TRPV1 channel sensitization following application of capsaicin (100nM for 1 minute) as a measure of neuronal excitability. Data was analyzed using chi-squared test as well as two-way and mixed-effects model ANOVA as appropriate, followed by Sidak’s multiple comparisons test.

Result(s)
IBS FS caused a 177% larger Ca2+ influx in response to capsaicin compared to HC FS in female mice (p=0.0148, N=6-7 mice, neurons=43-49). In male mice, IBS FS increased Ca2+ influx by only 13% compared to HC FS (p=0.79, N=5 mice, neurons=28-35). In female mice, 117% more neurons responded to capsaicin after incubation with IBS FS versus HC FS (p=0.0004), while in male mice, only 17% more neurons responded following incubation with IBS FS (p=0.46). Finally, FS from the same IBS patients following a LFD reduced neuronal Ca2+ influx by 39% compared to IBS FS in female mice (p=0.0434, N=4-6 mice, neurons=18-49). In male mice, LFD FS reduced Ca2+ influx by 11% versus IBS FS (p=0.98, N=5 mice, neurons=28-35).

Conclusion(s)
Nociceptive neurons from female mice are more sensitive to the pro-nociceptive effects of FS from IBS patients, as well as a reduction of these excitatory effects following the LFD. This suggests a potential role of sex hormones in pain signaling in IBS.

Disclosure of Interest
None Declared
A276 CANNABINOID RECEPTOR 1 IS LOCALIZED IN ENTERIC NEURONS AND EPITHELIUM IN FETAL AND EARLY POSTNATAL MOUSE INTESTINE
Authors: Sunil M , Adnan M , Farcas S , Pugazhendhi A , Ratcliffe E 
Published: March 2023

Abstract

Background
The endocannabinoid system (ECS) is necessary for gastrointestinal (GI) homeostasis and serves as a target for exogenous medicinal and recreational cannabinoid compounds. While components of the ECS, including receptors, ligands, and biosynthetic/degradative enzymes, have been increasingly characterized in the adult GI tract of both humans and animal models, similar advances in understanding the role of the ECS in GI developmental programming have not yet been made.

Purpose
We tested the hypothesis that the ECS plays a role in the developmental programming of the GI tract.

Method
The developmental timeline of ECS component expression was investigated in the GI tract from fetal and early postnatal mice. Samples from timed-pregnant outbred mice were collected from embryonic days 14 (E14), E16, E18 and postnatal days 1 (P1), P7 and P28, representing a timeline of early colonization of the GI tract by enteric neurons (E14) to 1 week after weaning (P28). Localization of cannabinoid receptor 1 (CB1) expression was examined in fetal and postnatal small intestine by immunohistochemistry. Fetal intestine was processed for RNA extraction and relative expression of ECS components determined by RT-qPCR.

Result(s)
CB1 expression at E14 was observed in the outer wall of the gut and in the developing mucosa. By E18, expression of CB1 became more localized to the enteric plexuses and to mucosal epithelial cells. In postnatal ileum, at least &gt;50% of myenteric ganglia were found to express CB1 from P1 to P28. CB1 expression continued to be present in the mucosal epithelium of ileum from P1 to P28, with a subset of CB1 expression in enterochromaffin cells, as identified by 5-HT immunolabeling. Significant decreases in expression of mRNA encoding CB1 (p&lt;0.0001) and the ECS biosynthetic enzymes NAPE-PLD (p&lt;0.05) and DAGLa (p&lt;0.05) were found between E14 and E18.

Conclusion(s)
Components of the ECS are present in the fetal and early postnatal GI tract. Further research is needed to better characterize the roles and mechanisms of action of the ECS in GI development. The potential impact of exogenous cannabis exposure in early life, either by in utero exposure or via the use of medicinal cannabinoids in childhood, highlights the importance of identifying the contributions of the ECS to normal development.

Disclosure of Interest
None Declared
A293 IS NON AL C OHOLIC FATTY PANCREATIC DISEASESE IS PATHOLOGICAL DISEASESE FOR DEVELOPMENT OF DIABETES :AN EGYPTIAN PILOT STUDY
Authors: Elbadawy R M , Natboli M S , Masoud G M 
Published: March 2023

Abstract

Background
Non Alcoholic Fatty Pancreatic disease(NAFPD), Fatty pancreas emerged as health problem world wide parralel to the epidemic of obesity .NAFPD lead chronic pancreatitis ,exocrine pancreatic insufficiency as per research proved . NAFPD also can lead to Diabeties Mellites (DM ) and lasly pancreatic adenocarcinoma . As per UEG cancer pancreas will be the the 3rd cancer world wide which is so serious and almost most of the patients come late with irriversible stage . Of sure most the patients with cancer pancreas have DM and other pancreatic disorders .

Purpose
The Aim of the research was to explore that NAFPD is the first stage in the development of DM

Method
Eighty -Eight subjects was inculded in the study both male and female ,DM or non DM and obese or not obese with mean age 25-66 years old .The subjects were divided into 4 groups .Group (1)DM subjects withnormal body mass index&lt;25kg/m2. Group (2)Subjects DM with body mass index &gt;25 kg/m2 obese .Group (3)subjects nonDM ,non obese with normal body mass index.Group (4)subjects non DM withbody mass index &gt;25kg/m2 . Each group 22 subjects both male and females.All routine investigations were done inculding lipid profile , liver functions .Nafpd was diagnosed by abdominal ultrasound ,Toshiba using convex probecompared to right kidney , liver and brightness of diaphragm .The echogencity was graded from (0-3 )..statistical analysis by SPSS version 22 was used.

Result(s)
Mean age was 44.08+_12.4 years old for all subjects. Mean Body Mass Index(BMI) was 29.73_+8.15 kg/m2 .Male was 73% while female 27% of NAFPD diagnosis .NAFPD was present in non obese non DM as follow ,grade 1,2,3(22.7%,27.3% and 22.7% respectively ).While NAFPD was present in DM non obese as follow in grades 1.2,3(22.7%,31.8% and45.4% respectively ) means that there was increas in % of DM development especially grade 2,3 . NAFPD was in obese non DM as follow in grades 2,3(31.8%,68.2% respectively . While in in DM obese NAFPD was present as follow (18.2%,81.2%). .This means that there was increaseincrease in % of DM development.

Conclusion(s)
NAFPD proved to be the first step in development of DM , it is an alert sign for health insitiute to care the issue to prevent DM from the start .
Aknowledgment to Academic Scientific Research & Technology for funding jesor project no 5269 , as this work part from the project

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None

Disclosure of Interest
R. Elbadawy Grant / Research support from: Aknowldgement to Academic Scientific Research & Technology , Egypt (ASRT ) for funding jesor project as this work part of the project, Paid Instructor of: 1000000 egyptian pound, M. Natboli: None Declared, G. Masoud: None Declared
The Disruptive Behavior Disorders Rating Scale: Updated Factor Structure, Measurement Invariance, and National Caregiver Norms
Authors: Fosco Whitney D , Babinski Dara E , Waschbusch Daniel A 
Published: March 2023

Abstract

Objective
To provide the first caregiver-report national norms for the Disruptive Behavior Disorders Rating Scale (DBDRS) and an updated evaluation of its factor structure and measurement invariance across child sex, informant sex, and child age.

Methods
Caregivers of children aged 5–12 years (N = 962) based in the United States completed the four DBDRS subscales. Using both severity scoring and dichotomous scoring procedures, confirmatory factor analyses supported a four-factor model of inattentive and hyperactive/impulsive symptoms, oppositional defiant symptoms, and conduct disorder symptoms.

Results
Measurement invariance was supported, indicating that the DBDRS functions similarly across demographic characteristics. Boys were reported to have more severe symptoms than girls (Cohen’s d = 0.33 [inattention], 0.30 [hyperactivity/impulsivity], 0.18 [oppositional defiant disorder], 0.14 [conduct disorder]), female caregivers rated ADHD symptoms as more severe than male caregivers (ds = 0.15 and 0.19 for inattention and hyperactivity/impulsivity, respectively), and older children were reported to experience more inattention than younger children (d = 0.18). Overall, group differences were modest in magnitude.

Conclusion
This psychometric study supports the continued use of the DBDRS in school-aged youth and will enhance the measure’s clinical and research utility by providing the first caregiver-report norms.
Debiased and thresholded ridge regression for linear models with heteroskedastic and correlated errors
Authors: Zhang Yunyi , Politis Dimitris N 
Published: March 2023

AbstractHigh-dimensional linear models with independent errors have been well-studied. However, statistical inference on a high-dimensional linear model with heteroskedastic, dependent (and possibly nonstationary) errors is still a novel topic. Under such complex assumptions, the paper at hand introduces a debiased and thresholded ridge regression estimator that is consistent, and is able to recover the model sparsity. Moreover, we derive a Gaussian approximation theorem for the estimator, and apply a dependent wild bootstrap algorithm to construct simultaneous confidence interval and hypothesis tests for linear combinations of parameters. Numerical experiments with both real and simulated data show that the proposed estimator has good finite sample performance. Of independent interest is the development of a new class of heteroscedastic, (weakly) dependent, and nonstationary random variables that can be used as a general model for regression errors.
Identifying optimally cost-effective dynamic treatment regimes with a Q-learning approach
Authors: Illenberger Nicholas , Spieker Andrew J , Mitra Nandita 
Published: March 2023

Abstract
Health policy decisions regarding patient treatment strategies require consideration of both treatment effectiveness and cost. We propose a two-step approach for identifying an optimally cost-effective and interpretable dynamic treatment regime. First, we develop a combined Q-learning and policy-search approach to estimate optimal list-based regimes under a constraint on expected treatment costs. Second, we propose an iterative procedure to select an optimally cost-effective regime from a set of candidate regimes corresponding to different cost constraints. Our approach can estimate optimal regimes in the presence of time-varying confounding, censoring, and correlated outcomes. Through simulation studies, we examine the operating characteristics of our approach under flexible modelling approaches. We also apply our methodology to identify optimally cost-effective treatment strategies for assigning adjuvant therapies to endometrial cancer patients.