bluuebunny/crossref_metadata_2025_split huggingface dataset:
The proneural transcription factor Atoh1 promotes odontogenic differentiation in human dental pulp stem cells (DPSCs)
Authors: Sabatini Camila , Lin Huey-Jiun , Ovik Galib , Hall Richard , Lee Techung 
Published: January 2025

Abstract

Background
Bioengineering of human teeth for replacement is an appealing regenerative approach in the era of gene therapy. Developmentally regulated transcription factors hold promise in the quest because these transcriptional regulators constitute the gene regulatory networks driving cell fate determination. Atonal homolog 1 (Atoh1) is a transcription factor of the basic helix-loop-helix (bHLH) family essential for neurogenesis in the cerebellum, auditory hair cell differentiation, and intestinal stem cell specification. The functional versatility of Atoh1 prompted us to test the possibility that Atoh1 may intersect the dental pulp stem cell (DPSC) gene regulatory network governing odontogenic differentiation.

Methods
We isolated DPSCs from human dental pulps and treated the cells with a replication-deficient adenoviral vector to achieve robust ectopic expression of Atoh1, following which the growth and odontogenic differentiation profiles of DPSCs were characterized.

Results
DPSCs harboring the Atoh1 expression vector exhibited an approximately 3,000-fold increase in the expression of Atoh1 compared to the negative control, leading to increased DPSC proliferation in the growth medium (P < 0.05). In the odontogenic medium, Atoh1 caused an early induction of BMP2 (P < 0.001) followed by a late induction of BMP7 (P < 0.01) and increased Wnt signaling (P < 0.01). The increased BMP/Wnt signaling led to up to 8-fold increased expression of the master osteogenic transcription factor Osterix (P < 0.005) while exhibiting no significant effect on Runx2 or Dlx5, which are abundantly expressed in DPSCs. Atoh1 stimulated expression of type I collagen (P < 0.005) and small integrin-binding ligand, N-linked glycoproteins (SIBLINGs) such as bone sialoprotein (P < 0.001), dentin matrix protein 1 (P < 0.05), dentin sialophosphoprotein (P < 0.005), and osteopontin (P < 0.001), resulting in increased dentin matrix mineralization (P < 0.05). The odontogenic phenotype is associated with metabolic remodeling marked by enhanced glycolytic flux and attenuated mitochondrial metabolic enzyme activities.

Conclusions
Atoh1, despite being a proneural transcription factor in development, possesses a novel odontogenic function upon ectopic expression in DPSCs. This in vitro study demonstrates a novel odontogenic mechanism mediated by ectopic expression of the transcription factor Atoh1 in human DPSCs. The finding may offer an innovative strategy for gene-based regeneration of the pulp-dentin complex.
NKX6.3 modulation of mitotic dynamics and genomic stability in gastric carcinogenesis
Authors: Yoon Jung Hwan , Kim Jeong-Kyu , Eun Jung Woo , Ashktorab Hassan , Smoot Duane T. , Nam Suk Woo , Park Won Sang 
Published: January 2025

Abstract

Background
Gastric cancer remains a significant global health challenge, characterized by poor prognosis and high mortality rates. Mitotic integrity and genomic stability are crucial in maintaining cellular homeostasis and preventing tumorigenesis. The transcription factor NKX6.3 has emerged as a potential regulator of these processes in gastric epithelial cells, prompting an investigation into its role in gastric cancer development.

Methods
We employed a combination of in vitro and in vivo techniques to elucidate the impact of NKX6.3 depletion on mitotic dynamics and genomic stability in gastric epithelial cells. Quantitative real-time PCR and Western blot analyses were conducted to assess the expression of mitosis-related genes and proteins. Flow cytometry was utilized to evaluate cell cycle distribution, while immunofluorescence microscopy enabled the visualization of mitotic abnormalities. Statistical analyses, including Student’s t-test and ANOVA, were performed to determine the significance of our findings.

Results
Our results demonstrate that NKX6.3 depletion leads to significant mitotic defects, characterized by increased chromosome misalignment and lagging chromosomes during anaphase. These abnormalities corresponded with elevated levels of genomic instability markers, indicating compromised genomic integrity. Furthermore, the loss of NKX6.3 resulted in altered expression of key regulatory proteins involved in mitosis and DNA repair pathways, suggesting a mechanistic link between NKX6.3 and the maintenance of genomic stability in gastric epithelial cells. Depletion of NKX6.3 resulted in accelerated cell cycle progression and the formation of abnormal mitotic figures, leading to genomic instability characterized by increased DNA content and structural abnormalities. In both in vitro and xenograft models, the depletion of NKX6.3 significantly upregulated AurkA and TPX2, which correlated with gains in DNA copy number. An inverse relationship was observed between NKX6.3 expression and the levels of AurkA and TPX2 in human gastric cancer tissues.

Conclusions
This study highlights the essential role of NKX6.3 in regulating mitotic integrity and genomic stability in gastric carcinogenesis. The findings suggest that targeting NKX6.3 may offer a novel therapeutic strategy for improving treatment outcomes in gastric cancer by restoring mitotic fidelity and genomic stability.

Trial registration
This study was not registered.
New-onset Type 1 Diabetes Mellitus Presenting as Bilateral Cataracts in a 15-year-old Girl
Authors: Gunaratne Naiomi , Tanenbaum Rebecca , Deplewski Dianne , Harris Marni , Rodriguez Sarah Hilkert 
Published: January 2025

Abstract
Cataracts secondary to type 1 or type 2 diabetes are not uncommon in adults; however, they are a rare finding in pediatric patients with type 1 diabetes. A 15-year-old girl presented with progressively worsened bilateral vision for 6 months. Her vision rapidly deteriorated over the previous month, prompting further evaluation that found bilateral cataracts with haziness in all layers and swollen lenses. Labs were done due to the findings and were significant for elevated serum glucose and hemoglobin A1c and mild diabetic ketoacidosis. Further testing confirmed type 1 diabetes. She had bilateral cataract surgery and has had a successful return of 20/20 vision in both eyes. The prevalence of early diabetic cataracts in the pediatric population is rare. Pathophysiology includes a defect in the polyol pathway, combined with oxidative stress, leading to increased fluid retention. Treatment involves cataract surgery and improved glycemic control. Current International Society for Pediatric and Adolescent Diabetes guidelines recommend initial evaluation for cataracts and subsequent surveillance concomitant with diabetic retinopathy monitoring biennially with those with good glycemic control. Given the rapid formation and severity of onset of bilateral cataracts for this patient, we propose continual screening for visual symptoms at each visit and emphasize the importance of ophthalmologic referrals.
Effects of Acute Exercise and Carbohydrate Intake on Plasma GDF-15 Levels and Its Association With Appetite Regulation
Authors: James Ellen , Frampton James , Murphy Kevin G , Chambers Edward S 
Published: January 2025

Abstract

Background
Growth differentiation factor 15 (GDF-15) is a potential therapeutic target for obesity due to its role in appetite suppression. Although acute exercise stimulates GDF-15 secretion, its relationship with appetite regulation remains unclear. It is also unknown whether preexercise carbohydrate intake would affect GDF-15 responses. This study aimed to examine the effects of acute exercise and carbohydrate intake on GDF-15 secretion and its potential links to appetite regulation.

Methods
In a secondary analysis of a randomized crossover trial, 12 healthy males completed four 120-minute trial conditions: (1) control (water) with rest, (2) control with exercise (0-30 minutes at ∼75% of maximal oxygen uptake), (3) carbohydrate (75 g maltodextrin) with rest, and (4) carbohydrate with exercise. Plasma GDF-15 levels were measured at 0, 30, 60, and 120 minutes, alongside subjective appetite ratings using visual analog scales. Energy intake was measured at the end of each trial condition with an ad libitum meal.

Results
Time-averaged area under the curve analysis showed that neither exercise [34 pg/mL (95% confidence interval [CI], −2-69 pg/mL); P = .062) nor carbohydrate intake [10 pg/mL (95% CI, −39-58 pg/mL); P = .673] independently or interactively (P = .283) affected GDF-15 levels. Exercise induced a delayed independent increase in GDF-15 at 120 minutes [55 pg/mL (95% CI, 18-94 pg/mL); P = .008]. No significant associations were found between GDF-15 levels and subjective appetite ratings or energy intake.

Conclusion
A 30-minute bout of high-intensity exercise induces a delayed increase in GDF-15 levels, which is not affected by carbohydrate intake. Physiological GDF-15 responses to acute exercise display no association with markers of appetite regulation.
Validity in Intercultural Interpretation of Romanticism: A Case Study of Qu Yuan’s Lisao
Authors: Li Qingben , Duo Duo 
Published: January 2025

Abstract
Whether Western theories can be employed to interpret Chinese literary texts and whether such interpretation is valid are two different propositions. Starting from an analysis of the relationship between Qu Yuan’s Lisao and Romanticism, in this paper, the thesis that “Qu Yuan is a Romantic” is thought to be a pseudo-proposition owing to its over-interpretation on Qu Yuan and his works, resulting in its invalidity. In order to achieve effective elucidation, it is necessary to change the statement from “Qu Yuan is a Romanticist” to “Qu Yuan and Romanticism” on the ground that we can neither deny the interpretability of Chinese texts via Western theory nor assert that “Qu Yuan is a Romanticist.”
ПРОГНОЗ СДВИЖЕНИЙ ЗЕМНОЙ ПОВЕРХНОСТИ НА ОСНОВЕ ИССЛЕДОВАНИЯ ДЕФОРМАЦИОННЫХ СВОЙСТВ ВОДОНАСЫЩЕННЫХ ГОРНЫХ ПОРОД
Authors: БОРИСЕНКО Э.В. , ГОЛУБЕВ Ф.М. , ПОПОВИЧ С.А. 
Published: January 2025

Приведены результаты экспериментальных исследований по водонасыщению образцов горных пород основных литологических типов, свойственных для Донбасса. Обобщены изменения деформационных свойств, происходящие в горных породах, при их увлажнении. Приведена методика разработки модели, позволяющей учитывать влияние затопления различных горных пород на параметры сдвижения. На основании лабораторных данных построена конечно-элементная модель активизации геомеханических процессов, вызванных подработкой и затоплением массива горных пород. Установлено влияние литологического состава массива на параметры сдвижения при затоплении горных выработок закрывающихся угледобывающих предприятий. Полученные результаты могут лечь в основу совершенствования действующих нормативных документов, регламентирующих прогноз сдвижений земной поверхности при добыче угля и ликвидации угольных шахт за счет учета литологической структуры углепородного массива.
The article describes experimental studies on water saturation of samples of major rock types typical of Donbass. The alterations of deformation properties of moist rocks are generalized. The procedure of modeling with regard to the effect of flooding on displacement parameters in different rocks is presented. Using laboratory data, a finite-element model of activation of geomechanical processes initiated by rock mass undermining and flooding is constructed. It is found how rock mass lithology influences displacement parameters in flooding of underground roadways in coal mines being closed. The research results can help improve effective regulatory documents on ground surface displacement prediction in coal mining and mine closure through lithological analysis of coal-rock mass.
ВЛИЯНИЕ СКОРОСТИ НАГРУЖЕНИЯ НА РАСПРЕДЕЛЕНИЕ НАКОПЛЕННОЙ ЭНЕРГИИ В УГОЛЬНО-ПОРОДНЫХ ОБРАЗЦАХ И ХАРАКТЕР ИХ РАЗРУШЕНИЯ
Authors: ЧЕН Г. , ЧЖАН Ц. , СЮЙ Ю. , ЛИ Т. , ВАН Э. , ЧЖАН Г. 
Published: January 2025

Выполнен анализ распределения энергии в угольно-породных образцах при их нагружении. Установлено, что разница накопленной энергии между углем и породой в основном зависит от их модулей упругости. На основе рассмотрения структурных характеристик и механических свойств угля и породы построена и проанализирована механическая модель угольно-породного тела и получена формула распределения энергии по компонентам. По результатам нагрузочных испытаний разработана энергетическая модель, позволяющая рассмотреть процесс деформирования угольно-породного тела. Предложен оценочный коэффициент склонности угольно-породного тела к ударному разрушению, учитывающий разницу энергии упругой деформации между компонентами и время высвобождения энергии. Этот коэффициент можно рассматривать в качестве критерия для оценки склонности угольно-породной среды к горному удару.
The difference in energy accumulation in coal and rock under the same stress level is analyzed, and it is found that the difference of energy accumulation of coal rock under the same stress level mainly depends on the elastic modulus of coal rock material. Based on the structural characteristics and mechanical properties of coal-rock, the mechanical model of combined coal-rock body was constructed and analyzed, and the calculation formula of partition energy storage of combined coal-rock body was deduced. The axial compression test of combined coal-rock body under different loading rates was designed and carried out. Finally, the differential energy instability model of the combined coal-rock body was constructed. An evaluation index of the impact tendency of the combined coal-rock body considering the elastic energy difference and the release time is proposed, which provides a multi-scale criterion for accurately evaluating the impact propensity of the combined body.
ГЕОМЕХАНИЧЕСКОЕ ОБОСНОВАНИЕ СПОСОБА УПРАВЛЕНИЯ СОСТОЯНИЕМ МАССИВА ПОРОД ЦЕЛИКАМИ И ОБРУШЕНИЕМ КРОВЛИ ПРИ ВЫЕМКЕ НАКЛОННЫХ РУДНЫХ ЗАЛЕЖЕЙ МАЛОЙ И СРЕДНЕЙ МОЩНОСТИ
Authors: НЕВЕРОВ А.А. , КОНУРИН А.И. , НЕВЕРОВ С.А. , ВАСИЧЕВ С.Ю. , ЩУКИН С.А. 
Published: January 2025

Приведены конструктивные особенности и дана оценка камерно-целикового порядка отработки рудных тел наклонного падения, совмещающего два разных способа управления горным давлением: за счет естественной устойчивости массива пород и обрушения налегающей толщи. Показано, что в тектонически напряженных массивах обеспечивается безопасность работ на стадии погашения камерных запасов и временного рудного целика под защитой нависающей породной консоли. Выявлено, что наклон залежи формирует преимущественно сдвиговой характер разрушения пород в целике и днище блока. Установлены рациональные соотношения минимальной ширины временного рудного целика и пролета камеры.
The authors design and estimate a room-and-pillar method for mining inclined orebodies with combination of two approaches to ground control: through natural stability of rock mass and by means of overlying rock caving. It is shown that in rock mass under tectonic stresses, it is safe to extract ore reserves from rooms and from temporal ore pillar under protection of rock overhang. Due to inclination of an ore body, rocks in the pillar and in the block bottom fail mostly by way of shearing. The efficient ratios of a minimal width of a temporal ore pillar to a span of a stope are determined.
ПРОГНОЗИРОВАНИЕ ПАРАМЕТРОВ БУРИМОСТИ ОСАДОЧНЫХ ГОРНЫХ ПОРОД
Authors: КАЯНИ Д.А. , ЗАХУР М.Х. , ИМАД М.З. , ШАХИД А.Ш.А. 
Published: January 2025

Отмечена важность характеризующих эффективность бурения показателей: индексов скорости бурения DRI, износа коронки BWI и долговечности резца CLI. Дан обзор известных моделей прогнозирования буримости горных пород по индексу скорости бурения и указано на недостаточность изученности долговечности коронок с помощью индекса износа коронки. Экспериментально исследовано влияние различных геомеханических параметров горных пород на буримость и износ коронки. Получена отрицательная линейная корреляцию индекса скорости бурения DRI и положительная линейная корреляция индекса износа коронки BWI со значениями почти всех рассмотренных геомеханических свойств пород за исключением пористости, с которой DRI имеет положительную корреляцию, а BWI- отрицательную. Разработаны статистические модели прогнозирования DRI и BWI (с R 2 ≥ 0.83) методами простой и множественной статистической регрессии.
Fundamental considerations like drillability and tool wear may be pondered for development of deep well drilling for oil and gas, and tunneling projects. Careful considerations of key parameters may enhance the advance rate, operational costs, activity completion timeline and inventory control for spares. NTNU/SINTEF developed drilling rate index (DRI), bit wear index (BWI) and cutter life index (CLI) to quantify drilling and TBM related parameters. Until now, most of the research work has been carried out to develop models in order to recognize the effects of rock properties on drillability. However, very little if no studies have been conducted on the prediction of bit wear using BWI. This work deals with the effects of different geo-mechanical parameters of rocks on the drillability and the bit wear. The work shows that both drillability and bit wear are affected by physical and strength parameters of rocks. Moreover, various statistical models (with R2 ≥ 0.83) have been developed through simple linear regression analysis (SLRA) and multiple linear regression analysis (MLRA) for the prediction of NTNU/SINTEF indices (DRI and BWI).
ОПРЕДЕЛЕНИЕ ИСТОЧНИКОВ МЕТАНОВЫДЕЛЕНИЯ В ОЧИСТНОЙ ЗАБОЙ УГОЛЬНЫХ ШАХТ АНАЛИЗОМ ИЗОТОПНОГО СОСТАВА УГЛЕРОДА МЕТАНА
Authors: ТАЙЛАКОВ О.В. , САЛТЫМАКОВ Е.А. , СОКОЛОВ С.В. , КОСТИНА А.В. , ПРОЦЕНКО Г.В. 
Published: January 2025

Предложен и опробован комплексный подход к оценке зоны влияния углепородного массива на очистной забой по газовому фактору, основанный на изучении изотопии шахтного метана. Установлено количественное соотношение изотопов углерода метана в угольной пробе и рудничной атмосфере в условиях семи действующих шахт Кузбасса. Определены геометрические параметры рассматриваемых зон, а также максимальное значение объема метана, которое может оказать нагрузку на очистной забой в ходе отработки выемочного участка. Показано существенное отличие объема газа и параметров зоны миграции метана при общепринятом и комплексном подходах с применением метода оценки изотопного состава углерода метана в рудничной атмосфере и угле рабочего горизонта.
The authors propose an approved integrated approach to assessing influence of coal-rock mass on coal faces by a gas-based criterion through the isotope analysis of coalbed methane. The quantitative ratios of carbon isotopes of methane are determined in coal samples and in mine air samples from seven operating mines in Kuzbass. The geometrical parameters of such zones are assessed, and the maximal methane volume capable to affect a coal face during its operation is found. It is emphasized that the gas volumes and the methane migration zone parameters differ greatly when found using a traditional method and the integrated approach with the carbon isotopic analysis of methane in mine air and in coal.
ОЦЕНКА ОСОБЕННОСТЕЙ КИНЕТИКИ ФЛОТАЦИИ КАЛЬЦИЕВЫХ МИНЕРАЛОВ ПРИ ВТОРИЧНОМ ОБОГАЩЕНИИ ФЛЮОРИТСОДЕРЖАЩЕГО СЫРЬЯ
Authors: КИЕНКО Л.А. , ВОРОНОВА О.В. 
Published: January 2025

Изучены особенности флотационного обогащения техногенных отходов карбонатно-флюоритовых руд Ярославской горнорудной компании. Выполнена оценка состава и технологических свойств сырья. Установлено, что содержащийся в них флюорит представлен минеральными сростками и шламовым материалом. Проблемы флотационного обогащения лежалых отходов обусловлены особыми свойствами поверхностей частиц, наличием пленочных покрытий и структурных преобразований. Рассмотрены перспективные способы воздействия на преобразование компонентов флотационной пульпы. Выявлено, что ультразвуковая и электрохимическая обработки технологических компонентов способствуют повышению показателей селективности разделения минералов. Проанализированы показатели обогащения в отдельные фиксированные промежутки времени в условиях специфических воздействий. При флотации по схемам, включающим ультразвуковую и электрохимическую подготовку пульпы, существенно увеличивается степень концентрации флюорита в пенных продуктах, возрастает селективность при разделении его с кальцитом. Извлечение флюорита в концентраты, содержащие более 94 % CaF2, увеличивается на 2.97 - 4.58 %. Показана возможность снижения массовой доли двуокиси кремния в концентратах на 0.3 - 0.5 %.
The research focuses on the specificity of flotation of carbonate-fluorite ore waste at Yaroslavskaya Mining Company. The waste composition and process properties are examined. Fluorite in the test waste occurs in mineral concretions and in slime. The problems in flotation of old mining and processing waste are governed by specific surface properties of particles, film coatings and structural transformations. The promising methods to induce conversion of components in flotation pulp are discussed. It is found that ultrasonic and electrochemical treatment of waste components promote selectivity of their separation. The indicators of processes at fixed time intervals and under special effects are analyzed. Flotation with ultrasonic and electrochemical pre-treatment of pulp substantially increases fluorite concentration in froth and enhances selectivity of fluorite separation from calcite. Recovery of fluorite in concentrates with CaF2 content more than 94% rises by 2.97-4.58%. It is demonstrated that it is possible to reduce mass fraction of silicon dioxide in concentrates by 0.3-0.5%.
Efficacy of octadecylamine-functionalized graphene versus graphene nanoplatelets and graphene oxide as asphalt binder modifiers for high-temperature performance
Authors: Almashaqbeh Hashem Khaled , Majdoub Mohammed , Sengottuvelu Dineshkumar , Nouranian Sasan , Doyle Jesse D. , Algharibeh Omar , Alkhateb Hunain , Rushing Grace , Al-Shraideh Nawal , Ucak-Astarlioglu Mine G. , Al-Ostaz Ahmed 
Published: January 2025

Abstract
In this work, we comprehensively report on the synthesis of octadecylamine (ODA)-functionalized graphene (G-ODA) and compare its performance to those of graphene nanoplatelets (GNPs) and graphene oxide (GO) as asphalt binder modifiers. An exploration into the mechanisms through which asphalt binder properties are enhanced by each graphene-based material has been conducted, identifying the one that demonstrates superior compatibility with asphalt. The study systematically evaluates the performance of each modifier, analyzing viscosity, rheology, anti-aging properties, morphology, and chemical transformations within asphalt binders. Experimental results indicate that all three graphene-based modifiers enhance the high-temperature performance and aging resistance of the asphalt binder, with GO emerging as the most compatible material, exhibiting superior performance across all investigated responses. The rheological properties results show that GO can improve G*/sin(δ) for the unaged binder by about 120%, followed by G-ODA and GNP. On the contrary, multiple stress creep and recovery (MSCR) results indicate that both GO and GNP efficiently reduce permanent deformation, with reductions in Jnr of about 39% and 34%, respectively while the G-ODA shows a smaller reduction of − 10%. Additionally, GO excels in improving elastic response, showing a substantial increase in percent recovery at 297%, compared to 48.4% with GNP and 28.8% with G-ODA. Fourier-transform infrared spectroscopy (FTIR) analysis establishes the absence of evidence indicating chemical interaction between any of the graphene-based materials and asphalt molecules. This suggests that the improvement is solely attributed to physical interaction, specifically through π-π interaction. On the other hand, AFM phase images indicate that all graphene-based materials can alter the morphology of asphalt binders. They increase the projected surface area of the Peri/Catana phases, which can influence the rheological properties of the binder.