bluuebunny/crossref_metadata_2025_split huggingface dataset:
The NightLife study — the clinical and cost-effectiveness of thrice-weekly, extended, in-centre nocturnal haemodialysis versus daytime haemodialysis using a mixed methods approach: study protocol for a randomised controlled trial
Authors: Hull Katherine L. , Bramham Kate , Brookes Cassandra L. , Cluley Victoria , Conefrey Carmel , Cooper Nicola J. , Eborall Helen , Fotheringham James , Graham-Brown Matthew P. M. , Gray Laura J. , Mark Patrick B. , Mitra Sandip , Murphy Gavin J. , Quann Niamh , Rooshenas Leila , Warren Madeleine , Burton James O. 
Published: August 2023

Abstract
Background
In-centre nocturnal haemodialysis (INHD) offers extended-hours haemodialysis, 6 to 8 h thrice-weekly overnight, with the support of dialysis specialist nurses. There is increasing observational data demonstrating potential benefits of INHD on health-related quality of life (HRQoL). There is a lack of randomised controlled trial (RCT) data to confirm these benefits and assess safety.

Methods
The NightLife study is a pragmatic, two-arm, multicentre RCT comparing the impact of 6 months INHD to conventional haemodialysis (thrice-weekly daytime in-centre haemodialysis, 3.5–5 h per session). The primary outcome is the total score from the Kidney Disease Quality of Life tool at 6 months. Secondary outcomes include sleep and cognitive function, measures of safety, adherence to dialysis and impact on clinical parameters. There is an embedded Process Evaluation to assess implementation, health economic modelling and a QuinteT Recruitment Intervention to understand factors that influence recruitment and retention. Adults (≥ 18 years old) who have been established on haemodialysis for > 3 months are eligible to participate.

Discussion
There are 68,000 adults in the UK that need kidney replacement therapy (KRT), with in-centre haemodialysis the treatment modality for over a third of cases. HRQoL is an independent predictor of hospitalisation and mortality in individuals on maintenance dialysis. Haemodialysis is associated with poor HRQoL in comparison to the general population. INHD has the potential to improve HRQoL. Vigorous RCT evidence of effectiveness is lacking. The NightLife study is an essential step in the understanding of dialysis therapies and will guide patient-centred decisions regarding KRT in the future.

Trial registration
Trial registration number: ISRCTN87042063. Registered: 14/07/2020.
Enhancement of β-carotene content in Chlamydomonas reinhardtii by expressing bacterium-driven lycopene β-cyclase
Authors: Huang Danqiong , Liu Chenglong , Su Mingshan , Zeng Zhiyong , Wang Chaogang , Hu Zhangli , Lou Sulin , Li Hui 
Published: August 2023

Abstractβ-Carotene is one of the economically important carotenoids, having functions as the antioxidant to remove harmful free radicals and as the precursor for vitamin A and other high-valued xanthophyll such as zeaxanthin and astaxanthin. Lycopene cyclase plays an important role in the branching of β-carotene and α-carotene. Aiming to develop the microalgae with enhanced β-carotene productivity, the CrtY gene from bacterium Pantoea agglomerans was integrated into Chlamydomonas reinhardtii. The lycopene-producing E. coli harboring CrtY gene produced 1.59 times of β-carotene than that harboring DsLcyb1 from Dunaliella salina (a microalga with abundant β-carotene), confirming the superior activity of CrtY on β-carotene biosynthesis. According to the pigment analysis by HPLC, in microalgal transformants that were confirmed by molecular analysis, the expression of CrtY significantly increased β-carotene content from 12.48 mg/g to 30.65 mg/g (dry weight), which is about 2.45-fold changes. It is noted that three out of five transformants have statistically significant higher amount of lutein, even though the increment was 20% in maximum. Besides, no growth defect was observed in the transformants. This is the first report of functional expression of prokaryotic gene in eukaryotic microalgae, which will widen the gene pool targeting carotenoids biosynthesis using microalgae as the factory and thereby provide more opportunity for high-valued products engineering in microalgae.
Reassessing Stephanofilaria stilesi dermatitis in cattle, with characterization of molecular markers for confirming diagnosis
Authors: Lui Clinson C. , Kulpa Matthew , Verocai Guilherme G. , Armién Aníbal G. , Edwards Erin E. , Wiener Dominique J. , Rech Raquel R. 
Published: August 2023

AbstractBackgroundStephanofilaria stilesiis a vector-borne filarioid nematode of cattle in North America that is transmitted via the hematophagous horn fly (Haematobia irritans) intermediate host. Despite being relatively common, little attention has been given to a thorough description ofS. stilesilesions and the potential integration of pathological and molecular diagnostic findings to confirm infection.MethodsTo characterize the cutaneous lesions caused byS. stilesiin cattle (Bos taurus taurusandBos taurus indicus), skin of the ventral abdominal midline was collected from 22 animals during postmortem examination. Skin samples were processed for histology, transmission electron microscopy (TEM), DNA extraction, PCR, and Sanger sequencing targeting molecular markers cytochrome oxidase c subunit 1 (cox1), 12S, 18S rDNA, and 28S rDNA.ResultsMacroscopically, lesions ranged from 5 × 4 cm to 36 × 10 cm, consisting of one large single lesion, or two to four ovoid areas at the ventral abdominal midline, surrounding the umbilicus. Each lesion presented as ulcerative dermatitis with dry, serocellular crusts, or alopecic and lichenified areas. Histologically, eosinophilic, neutrophilic, and ulcerative dermatitis with furunculosis, folliculitis, and epidermal hyperplasia was observed. Cross sections of adult nematodes were identified in ~ 60% of the cases (n = 13) within intact follicles, sebaceous ducts, crusts, and areas of furunculosis.Stephanofilariafirst-stage larvae (L1) were observed in five cases within “vitelline membranes” in the superficial dermis and crusts. Ultrastructurally, the L1 cross sections were compounded of smooth multilayered cuticle and somatic cells. The “vitelline membrane” is a tri-layered membrane where L1 are suspended in a matrix.Stephanofilaria stilesiDNA was found in 5 out of the 13 cases in which adults or L1 were histologically observed (38%) and in 1 out of the 9 cases without adults or L1 present (11%). Phylogenetic analyses suggest a closer relationship of the genusStephanofilariawith Thelazioidea, instead of the family Filariidae (Filarioidea), in which it has been historically allocated.ConclusionsOur study improved the characterization of lesions and described ultrastructural findings ofS. stilesiand highlights that molecular tools should be utilized in combination with histology for improved diagnostic resolution.Graphical Abstract
A meta-analysis of the criterion-related validity of Session-RPE scales in adolescent athletes
Authors: Liu Haochong , Yang Wenpu , Liu Haoyang , Bao Dapeng , Cui Yixiong , Ho Indy Man Kit , Li Qian 
Published: August 2023

Abstract
Background
The objective of this study was to establish the criterion-related validity of the session-rating of perceived exertion (s-RPE) method in adolescent athletes.

Methods
According to the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA 2020) guidelines, a meta-analysis (PROSPERO ID: CRD42022373126) was performed using Stata 15.1 software. Eight databases using the following terms: (‘s-RPE’ OR ‘Rating Perceived Exertion session’ OR ‘RPE session’ OR ‘RPE’ OR ‘Rate of Perceived Exertion’ OR ‘Rated of Perceived Exertion’) AND (‘Adolescen*’ OR ‘Youth*’ OR ‘Teen*’) AND (‘validity’ OR ‘correlation’ OR ‘concurrent validity’) were searched up to 2022. Articles meeting the inclusion criteria were screened and adopted the “Methodological Index for Non-Randomized Studies (MINORS)” to evaluate the risk of bias.

Results
An initial 1798 studies using the s-RPE method were identified and finally, a total of 16 studies were included for further analysis. The relationship between assessment instruments CR-10 or CR-100 modified methods of s-RPE and the heart rate measures of these selected studies were calculated using correlation coefficient (r values) and Fisher’s z-score. A strong to very strong correlation between s-RPE and HR was observed (overall: r = 0.74; CR-10: r = 0.69; CR-100: r = 0.80). CR-100 scale (Fisher’s z = 1.09) was shown to have a higher criterion validity than that of the CR-10 scale (Fisher’s z = 0.85).

Conclusion
Preliminary findings showed that s-RPE using either CR-10 or CR-100 scales can be used "stand-alone" for monitoring internal training load for children and adolescent athletes. Future studies should focus on whether CR-100 could better perform than CR-10 for junior and children athletes in different age groups and sports as well as the causes leading to potential scoring biases.
Left ventricle endomyocardial fibrosis: a case report
Authors: Soares Raquel Reis , Avelar Maria Clara Martins , Zanetti Sofia Lucena , Garreto Joao Victor Tavares Mendonça , Guimaraes Vinicius Dinelli , Ferber Elisa Soares , de Oliveira Drumond Mayumi , Ferber Matheus , Ferber Leonardo 
Published: August 2023

Abstract
Background
Endomyocardial fibrosis is a grim disease. It is the most common restrictive cardiomyopathy worldwide, but the exact etiology and pathogenesis both remain unknown. Endomyocardial fibrosis is recurrently associated with chronic eosinophilia and probable dietary, environmental, and infectious factors, which contribute not only to the onset of the disease (an inflammatory process) but also to its progression and maintenance (endomyocardial damage and scar formation). The trademark of the disease is the fibrotic obliteration of the affected ventricle. The combination of such processes produces focal or diffuse endocardial thickening and fibrosis, which leads to restrictive physiology. Endomyocardial fibrosis affects the apices of the right and the left ventricle in around 50% of cases and most often extends to the posterior leaflet of the mitral valve. Sometimes it involves the papillary muscle and chordae tendineae, causing atrioventricular valve dysfunction. The fibrosis does not affect extracardiac organs. This cardiomyopathy is most recurrent in tropical areas of the world.

Case presentation
A 67-year-old Black male with past medical history of schistosomiasis infection in childhood presented with progressive dyspnea, lower extremity edema, and weakness for 2 years. He was diagnosed with endomyocardial fibrosis. The echocardiogram showed an increased thickness in the septum (17 mm) and free left ventricular wall (15 mm), obliteration of the left ventricular apex and inflow tract, and mitral valve regurgitation. Cardiac magnetic resonance imaging revealed apical left ventricle wall thickening with left ventricular apical obliteration associated with enlargement of the respective atrium. Delayed enhancement imaging showed endomyocardium enhancement involving left ventricular apex, mitral valve regurgitation due to annulus dilation, and a thrombus at left ventricular apex. He underwent open heart surgery with mitral valve replacement, endocardial decortication, endomyocardiectomy, and two-vessel coronary artery bypass grafting as preoperative coronary angiogram showed mild right coronary artery and proximal left anterior descending artery severe lesions. Postoperative course was uncomplicated, and he was discharged successfully from the hospital. Six months after surgery, he was New York Heart Association functional class I.

Conclusion
The purpose of this case report is to illustrate the aspects of endomyocardial fibrosis by reporting a case of this entity. In conclusion, progress in imaging techniques and treatment in a reference institution for cardiac diseases contribute to earlier diagnosis and survival in patients with endomyocardial fibrosis.
Fusion of myofibre branches is a physiological feature of healthy human skeletal muscle regeneration
Authors: Højfeldt Grith , Sorenson Trent , Gonzales Alana , Kjaer Michael , Andersen Jesper L. , Mackey Abigail L. 
Published: August 2023

Abstract
Background
The occurrence of hyperplasia, through myofibre splitting, remains a widely debated phenomenon. Structural alterations and fibre typing of skeletal muscle fibres, as seen during regeneration and in certain muscle diseases, can be challenging to interpret. Neuromuscular electrical stimulation can induce myofibre necrosis followed by changes in spatial and temporal cellular processes. Thirty days following electrical stimulation, remnants of regeneration can be seen in the myofibre and its basement membrane as the presence of small myofibres and encroachment of sarcolemma and basement membrane (suggestive of myofibre branching/splitting). The purpose of this study was to investigate myofibre branching and fibre type in a systematic manner in human skeletal muscle undergoing adult regenerative myogenesis.

Methods
Electrical stimulation was used to induce myofibre necrosis to the vastus lateralis muscle of one leg in 5 young healthy males. Muscle tissue samples were collected from the stimulated leg 30 days later and from the control leg for comparison. Biopsies were sectioned and stained for dystrophin and laminin to label the sarcolemma and basement membrane, respectively, as well as ATPase, and antibodies against types I and II myosin, and embryonic and neonatal myosin. Myofibre branches were followed through 22 serial Sects. (264 μm). Single fibres and tissue blocks were examined by confocal and electron microscopy, respectively.

Results
Regular branching of small myofibre segments was observed (median length 144 μm), most of which were observed to fuse further along the parent fibre. Central nuclei were frequently observed at the point of branching/fusion. The branch commonly presented with a more immature profile (nestin + , neonatal myosin + , disorganised myofilaments) than the parent myofibre, together suggesting fusion of the branch, rather than splitting. Of the 210 regenerating muscle fibres evaluated, 99.5% were type II fibres, indicating preferential damage to type II fibres with our protocol. Furthermore, these fibres demonstrated 7 different stages of “fibre-type” profiles.

Conclusions
By studying the regenerating tissue 30 days later with a range of microscopy techniques, we find that so-called myofibre branching or splitting is more likely to be fusion of myotubes and is therefore explained by incomplete regeneration after a necrosis-inducing event.
In vitro antibiofilm and bacteriostatic activity of diacerein against Enterococcus faecalis
Authors: Fu Chunyan , Xu Yuxi , Zheng Hao , Ling Xinyi , Zheng Chengzhi , Tian Leihao , Gu Xiaobin , Cai Jiabei , Yang Jing , Li Yuanyuan , Wang Peiyu , Liu Yuan , Lou Yongliang , Zheng Meiqin 
Published: August 2023

AbstractEnterococcus faecalis is one of the main pathogens that causes hospital-acquired infections because it is intrinsically resistant to some antibiotics and often is capable of biofilm formation, which plays a critical role in resisting the external environment. Therefore, attacking biofilms is a potential therapeutic strategy for infections caused by E. faecalis. Current research indicates that diacerein used in the treatment of osteoarthritis showed antimicrobial activity on strains of gram-positive cocci in vitro. In this study, we tested the MICs of diacerein using the broth microdilution method, and successive susceptibility testing verified that E. faecalis is unlikely to develop resistance to diacerein. In addition, we obtained a strain of E. faecalis HE01 with strong biofilm-forming ability from an eye hospital environment and demonstrated that diacerein affected the biofilm development of HE01 in a dose-dependent manner. Then, we explored the mechanism by which diacerein inhibits biofilm formation through qRT-PCR, extracellular protein assays, hydrophobicity assays and transcriptomic analysis. The results showed that biofilm formation was inhibited at the initial adhesion stage by inhibition of the expression of the esp gene, synthesis of bacterial surface proteins and reduction in cell hydrophobicity. In addition, transcriptome analysis showed that diacerein not only inhibited bacterial growth by affecting the oxidative phosphorylation process and substance transport but also inhibited biofilm formation by affecting secondary metabolism, biosynthesis, the ribosome pathway and luxS expression. Thus, our findings provide compelling evidence for the substantial therapeutic potential of diacerein against E. faecalis biofilms.
Vancomycin-resistant enterococci (VRE) in hospital settings across European borders: a scoping review comparing the epidemiology in the Netherlands and Germany
Authors: Cimen Cansu , Berends Matthijs S. , Bathoorn Erik , Lokate Mariëtte , Voss Andreas , Friedrich Alex W. , Glasner Corinna , Hamprecht Axel 
Published: August 2023

AbstractThe rising prevalence of vancomycin-resistant enterococci (VRE) is a matter of concern in hospital settings across Europe without a distinct geographical pattern. In this scoping review, we compared the epidemiology of vancomycin-resistant Enterococcus spp. in hospitals in the Netherlands and Germany, between 1991 and 2022. We searched PubMed and summarized the national antibiotic resistance surveillance data of the two countries. We included 46 studies and summarized national surveillance data from the NethMap in the Netherlands, the National Antimicrobial Resistance Surveillance database in Germany, and the EARS-Net data. In total, 12 studies were conducted in hospitals in the Netherlands, 32 were conducted in German hospitals, and an additional two studies were conducted in a cross-border setting. The most significant difference between the two countries was that studies in Germany showed an increasing trend in the prevalence of VRE in hospitals, and no such trend was observed in studies in the Netherlands. Furthermore, in both Dutch and German hospitals, it has been revealed that the molecular epidemiology of VREfm has shifted from a predominance of vanA towards vanB over the years. According to national surveillance reports, vancomycin resistance in Enterococcus faecium clinical isolates fluctuates below 1% in Dutch hospitals, whereas it follows an increasing trend in German hospitals (above 20%), as supported by individual studies. This review demonstrates that VRE is more frequently encountered in German than in Dutch hospitals and discusses the underlying factors for the difference in VRE occurrence in these two neighboring countries by comparing differences in healthcare systems, infection prevention control (IPC) guidelines, and antibiotic use in the Netherlands and Germany.
Hepatic steatosis is associated with dysregulated cholesterol metabolism and altered protein acetylation dynamics in chickens
Authors: Guo Xiaoli , Zhou Qianqian , Jin Jiaming , Lan Fangren , Wen Chaoliang , Li Junying , Yang Ning , Sun Congjiao 
Published: August 2023

Abstract
Background
Hepatic steatosis is a prevalent manifestation of fatty liver, that has detrimental effect on the health and productivity of laying hens, resulting in economic losses to the poultry industry. Here, we aimed to systematically investigate the genetic regulatory mechanisms of hepatic steatosis in laying hens.

Methods
Ninety individuals with the most prominent characteristics were selected from 686 laying hens according to the accumulation of lipid droplets in the liver, and were graded into three groups, including the control, mild hepatic steatosis and severe hepatic steatosis groups. A combination of transcriptome, proteome, acetylome and lipidome analyses, along with bioinformatics analysis were used to screen the key biological processes, modifications and lipids associated with hepatic steatosis.

Results
The rationality of the hepatic steatosis grouping was verified through liver biochemical assays and RNA-seq. Hepatic steatosis was characterized by increased lipid deposition and multiple metabolic abnormalities. Integration of proteome and acetylome revealed that differentially expressed proteins (DEPs) interacted with differentially acetylated proteins (DAPs) and were involved in maintaining the metabolic balance in the liver. Acetylation alterations mainly occurred in the progression from mild to severe hepatic steatosis, i.e., the enzymes in the fatty acid oxidation and bile acid synthesis pathways were significantly less acetylated in severe hepatic steatosis group than that in mild group (P < 0.05). Lipidomics detected a variety of sphingolipids (SPs) and glycerophospholipids (GPs) were negatively correlated with hepatic steatosis (r ≤ −0.5, P < 0.05). Furthermore, the severity of hepatic steatosis was associated with a decrease in cholesterol and bile acid synthesis and an increase in exogenous cholesterol transport.

Conclusions
In addition to acquiring a global and thorough picture of hepatic steatosis in laying hens, we were able to reveal the role of acetylation in hepatic steatosis and depict the changes in hepatic cholesterol metabolism. The findings provides a wealth of information to facilitate a deeper understanding of the pathophysiology of fatty liver and contributes to the development of therapeutic strategies.
First description of courtship and copulation in Pristidactylus casuhatiensis (Squamata, Leiosauridae)
Authors: Lujan Ogeda D. B. , Areco A. , Leynaud G. C. , Zalba S. M. 
Published: August 2023

Abstract
Background
Reproductive behaviour under natural conditions is hard to record, especially in rare and cryptic species, such as the Casuhatien anole (Pristidactylus casuhatiensis). This medium size lizard is endemic to the Sierras Australes Bonaerenses, a rocky outcrop in the Southern Pampas of Argentina.

Methods
During spring and summer from 2018 to 2022, we surveyed five hills at above 600 m altitude, where specimens were known to occur. Every time we detected a courtship or copulation event, we took photographs of the specimens for their individual identification, and recorded the body temperature of the participating individuals as well as air temperature, wind speed and relative humidity. We recorded the duration in minutes of each phase of the event (immobilization, juxtaposition of the cloacae followed by copulation, and finally, restraint and separation) and described the main habitat features of the site where it happened.

Results
We detected 12 reproductive events; all of them occurred in October and November, on Funke, Puntudo and Tres Picos hills, mostly between 1300 and 1700 h. The mean duration of each copulation phase was 6 min (95% CI =  ± 1.4, n = 10), 4.3 min (95% CI =  ± 1.8, n = 10) and 3.8 min (95% CI =  ± 3.42, n = 10), respectively.

Conclusion
The reproductive activity of the Casuhatien anole is concentrated in the spring, particularly in the early afternoon, following the patterns described for other species of lizards. During these events, the greatest exposure of individuals could make them more vulnerable. This, together with other features related to their reproductive behaviour revealed in this paper may contribute to the planning of conservation actions.