bluuebunny/crossref_metadata_2025_split huggingface dataset:
Biological and Sensing Applications of a Few 1,3,4-Oxadiazole Based Donor-Acceptor Systems
Authors: M Remya T , M Asha T , Deepti Ayswaria , Prakash Prabha , P.S Baby Chakrapani , P.A Unnikrishnan 
Published: January 2023

Abstract
1,3,4-Oxadiazole pharmacophore is still considered a viable biologically active scaffold for the synthesis of more effectual and broad-spectrum antimicrobial agents. Therefore, the present study is based on five 1,3,4-oxadiazole target structures, viz, CAROT, CAROP, CARON (D-A-D-A systems) and NOPON and BOPOB (D-A-D-A-D systems) bearing various bioactive heterocyclic moieties relevant to potential biological activities. Three of the compounds, CARON, NOPON and BOPOB were assessed in-vitro for their efficacy as antimicrobial agents against gram positive (Staphylococcus aureus and Bacillus cereus) and gram negative (Escherichia coli and Klebsiella pneumonia) bacteria; and two fungi, Aspergillus niger and Candida albicans; also, as an anti-tuberculosis agent against Mycobacterium tuberculosis. Most of the tested compounds displayed promising antimicrobial activity, especially CARON which was then analyzed for the minimum inhibitory concentration (MIC) studies. Similarly, NOPON portrayed the highest anti-TB activity among the studied compounds. Consequently, to justify the detected anti-TB activity of these compounds and to recognize the binding mode and important interactions between the compounds and the ligand binding site of the potential target, these compounds were docked into the active binding site of cytochrome P450 CYP121 enzyme of Mycobacterium tuberculosis, 3G5H. The docking results were in good agreement with the result of in-vitro studies. In addition, all the five compounds were tested for their cell viability and have been investigated for cell labeling applications. To conclude, one of the target compounds, CAROT was used for the selective recognition of cyanide ion by ‘turn-off’ fluorescent sensing technique. The entire sensing activity was examined by spectrofluorometric method and MALDI spectral studies. The limit of detection obtained was 0.14 μM.
TM4SF1-AS1 inhibits apoptosis by promoting stress granule formation in cancer cells
Authors: Kitajima Hiroshi , Maruyama Reo , Niinuma Takeshi , Yamamoto Eiichiro , Takasawa Akira , Takasawa Kumi , Ishiguro Kazuya , Tsuyada Akihiro , Suzuki Ryo , Sudo Gota , Kubo Toshiyuki , Mitsuhashi Kei , Idogawa Masashi , Tange Shoichiro , Toyota Mutsumi , Yoshido Ayano , Kumegawa Kohei , Kai Masahiro , Yanagihara Kazuyoshi , Tokino Takashi , Osanai Makoto , Nakase Hiroshi , Suzuki Hiromu 
Published: January 2023

Abstract
Long noncoding RNAs (lncRNAs) play pivotal roles in tumor development; however, little is known about their involvement in the early stages of tumorigenesis. To identify dysregulated lncRNAs in precancerous lesions, we analyzed genome-wide trimethylation of histone H3 lysine 4 (H3K4me3) to screen for transcriptionally active lncRNA genes in the non-tumorous gastric mucosa of patients with gastric cancer (GC) and healthy individuals. We found that H3K4me3 at TM4SF1-AS1 was specifically upregulated in GC patients and that the expression of TM4SF1-AS1 was significantly elevated in primary and cultured GC cells. TM4SF1-AS1 contributes to GC cell proliferation in vitro and in vivo, and its oncogenic function is mediated, at least in part, through interactions with purine-rich element-binding protein α (Pur-α) and Y-box binding protein 1 (YB-1). TM4SF1-AS1 also activates interferon signaling in GC cells, which is dependent on Pur-α and RIG-I. Chromatin isolation by RNA purification (ChIRP)-mass spectrometry demonstrated that TM4SF1AS1 was associated with several stress granule (SG)-related proteins, including G3BP2, RACK1, and DDX3. Notably, TM4SF1-AS1 promoted SG formation and inhibited apoptosis in GC cells by sequestering RACK1, an activator of the stress-responsive MAPK pathway, within SGs. TM4SF1AS1-induced SG formation and apoptosis inhibition are dependent on Pur-α and YB-1. These findings suggested that TM4SF1-AS1 contributes to tumorigenesis by enhancing SG-mediated stress adaptation.
Probabilistic mapping of gait changes after STN-DBS for Parkinson’s disease
Authors: Reich Martin , Nickl Robert , Grossmann Anne , Roothans Jonas , Navrátil Pavel , Isaias Ioannis , Lange Lukas , Fricke Patrick , Capetian Philipp , Matthies Cordula , Volkmann Jens , Eldebakey Hazem 
Published: January 2023

Abstract
Objective.
Gait disturbances causing impaired mobility are common in Parkinson’s disease after bilateral deep brain stimulation of the subthalamic nucleus. We describe subthalamic subregions where neurostimulation had a positive effect on gait or provoked gait disturbances.
Methods.
Sixty-eight patients were classified according to postoperative gait changes: (1) gait improvement, (2) no change, (3) de novo gait disturbances. We performed a segregation analysis for (1) and (3) by simulating volumes of tissue activated and comparing aggregated spatial data for the two groups and calculated probability maps to forecast gait performance and the parkinsonism control.
Results.
Twenty patients experienced complete remission of presurgical gait problems after stimulation. Nine patients showed de novo gait disturbances one year post-implantation. Active contacts were more ventrally located for de novo gait disturbances versus gait improvement. Strong correlations were found between clinical alterations in gait and the individual stimulation volume within the probabilistic outcome gait map (R2 = 0.78; p = 0.01), whereby clinical improvement in parkinsonism correlated with individual stimulation volume within the corresponding probabilistic outcome map (R2 = 0.39; p = 0.01). The probabilistic maps predict patients who experience long-term gait benefits based on their volume of tissue activated overlap, which was gait specific and showed no correlation with the global parkinsonism control heatmap.
Interpretation.
Probabilistic mapping showed high correlation for therapy outcomes, especially gait improvement. The concept of sweet- or badspots could not explain individual differences. The thin delineations between close substructures in the subthalamic nucleus correlated with individual gait changes after neurostimulation. Probabilistic mapping may direct future re-programming approaches for greater mobility in parkinsonian patients.
Local and terminal cell differentiation mediated by the jasmonic acid-mediated autophagy evokes petal abscission in Arabidopsis thaliana
Authors: Yamamoto Haruka , Furuta Yuki , Hirakawa Takehi , Uemura Akira , Pelayo Margaret , Iimura Hideaki , Katagiri Naoya , Takeda-Kamiya Noriko , Kumaishi Kie , Ishiguro Sumie , Ichihashi Yasunori , Suzuki Takamasa , Goh Tatsuaki , Toyooka Kiminori , Ito Toshiro , Yamaguchi Nobutoshi 
Published: January 2023

Abstract
In angiosperms, transition from floral organ maintenance to abscission determines plant reproductive success and seed dispersion. For petal abscission, local cell fate decision at petal cell base is more important than general senescence or cell death of petals at organ levels. However, how this decision is regulated in a spatiotemporal manner remains unclear. Here, we describe a jasmonic acid (JA)–regulated chromatin state change that directs the local cell fate determination via autophagy at the base of petals. During petal maintenance phase, transcriptional co-repressors of JA signaling accumulate at the base of petals to block MYC activity, leading to lower reactive oxygen species (ROS) levels. JA acts as an airborne signaling molecule transmitted from stamens to petals and accumulates primarily in petal bases to trigger epigenetic reprograming, allowing MYC transcription factors to interact with MEDIATOR25 and to increase chromatin accessibility for key downstream targets, such as Arabidopsis NAC DOMAIN-CONTAINING PROTEIN102 (ANAC102). ANAC102 specifically accumulates at the base of petals just prior to petal abscission and triggers local ROS accumulation and cell death via AUTOPHAGY-RELATED GENES induction. Notably, this ANAC102-triggered autophagy at the petal base causes maturation, vacuolar delivery, and breakdown of autophagosome for terminal cell differentiation. Because dynamic changes in the number of vesicles and cytoplasmic components in the vacuole are often seen in many plants, the local cell fate determination by autophagy may be conserved for petal abscission across angiosperms and may have been recruited under the JA/NAC module to pattern plant organ detachment systems.
Room Temperature Fabrication of Cobalt Mullite for the Snappy Adsorption of Cationic and Anionic Dyes
Authors: Lakshmi G.Vidhya , Chellapandian Kannan 
Published: January 2023

Abstract
Cobalt mullite adsorbent for the robust adsorption performance towards Victoria Blue (VB) and Metanil Yellow (MY) is fabricated by the sol-gel method at room temperature using dipropylamine as a structure-directing agent. The synthesized adsorbent is characterized by XRD, FT-IR and HRTEM. From these analyses, it is found that dipropylamine binds with the alumina and cobalt oxide which makes it into tetrahedral to octahedral form. This interaction causes the formation of cobalt mullite. It is observed that trigonal alumina and orthorhombic cobalt mullite are interlinked to form a hybrid network. The special feature of adopting this adsorbent for the adsorption of VB and MY is that it has a large amount of Brønsted acid site because of the octahedral coordination of Al and Co. The large availability of acid sites in the framework and hybridization of two different network systems favors robust adsorption. The rate of adsorption (K2 = 0.00402 g/mg.min for VB and K2 = 0.004 g/mg.min for MY) and adsorption capacity (Qe=102.041 mg/g for VB and Qe=19.0406 mg/g for MY) are greater for VB than MY. This may be due to the more steric factor involved in MY than VB. Thermodynamic parameter indicated that the adsorption of VB and MY is spontaneous, endothermic and increased randomness in the adsorbent-adsorbate interface. The results from the enthalpy value (ΔHº = 65.43 kJ/mol for VB and ΔHº = 44.729 kJ/mol for MY) revealed that the chemisorption is involved in the adsorption process.
Isolation of Endophytic Bacteria and Its Commercial Application as PGP for Sustainable Agriculture to Improve Crop Yield.
Authors: Ghorpade Suhas Madhukar , Gorwadkar Vrushali Rajendra , Kharmate Aishwarya Vilas 
Published: January 2023

AbstractBackground Utilization of endophytic bacteria in order to increase productivity may be viable alternative to organic fertilizers. The goal is to reduce soil pollution and to preserve environment in the spirit of ecological agriculture. Any organism that at some part of its life cycle, colonize the internal plant tissues without causing any type of harm to the host plant are endophytes. Endophytes are known to promote plant growth and nutrient gain. They may improve the plant’s ability to tolerate various types of abiotic and biotic stresses, and enhance the resistance of plants to insects and pests. This study was carried out to isolate beneficial endophytic bacteria from various sources and to screen and identify plant growth promoting activities of isolated bacteria. Further to study field effects of best found endophytic bacterial isolates and its commercial application. Sources were collected from different regions of Maharashtra such as Geolife Research Farm Pune, Mangrove leaves from Mumbai, Khopoli ghat and examined for their bacterial diversity. Isolated bacteria subjected for various tests such as Indole Acetic Acid production, Ammonia production, Hydrogen Cyanide production, Antibiotic resistance and sensitivity assay, Proteolytic and Amylolytic activities, etc. Results In total 34 bacterial isolates were obtained from five different source. Some isolates were found to exhibit multiple beneficial characteristics to promote plant growth. Among them isolate number E14 has shown maximum production of IAA, same isolate also found positive for ammonia production and HCN production. Whereas isolate number E23 obtained from mangrove leaves is showing maximum nitrogen 12.6mg, positive for Ammonia & HCN production and also showing positive results for Amylolytic and Proteolytic activities. Conclusion According to field results, the interaction between plant and endophytes can promote plant immunity and play a significant role in sustainable agriculture. Endophytes displaying beneficial traits that can be exploited in agriculture biotechnology.
Dysfunctional serotonergic neuron-astrocyte signaling in depressive-like states
Authors: Perea Gertrudis , Gonzalez-Arias Candela , Esparza Julio , Sanchez-Puelles Cristina , Arancibia Lucia , Sanchez-Ruiz Andrea , Ramirez-Franco Jorge , Gobbo Davide , Kirchhoff Frank 
Published: January 2023

Abstract
Astrocytes play crucial roles in brain homeostasis and are regulatory elements of neuronal and synaptic physiology. Astrocytic alterations have been found in Major Depressive Disorder (MDD) patients; however, the consequences of astrocyte Ca2+ signaling in MDD are poorly understood. Here, we found that corticosterone-treated mice (Cort-mice) showed altered astrocytic Ca2+ dynamics in mPFC both in resting conditions and during social interactions, in line with altered mice behavior. Additionally, Cort-mice displayed reduced serotonin (5-HT)-mediated Ca2+ signaling in mPFC astrocytes, and aberrant 5-HT-driven synaptic plasticity in layer 2/3 mPFC neurons. Downregulation of astrocyte Ca2+ signaling in naïve animals mimicked the synaptic deficits found in Cort-mice. Remarkably, boosting astrocyte Ca2+ signaling with Gq-DREADDS restored mood and cognitive deficits in Cort-mice to control levels. This study highlights the important role of astrocyte Ca2+ signaling for homeostatic control of brain circuits and behavior, but also reveals its potential therapeutic value for depressive-like states.
Spaceflight alters insulin and estrogen signaling pathways
Authors: Mathyk Begum Aydogan , Tabetah Marshall , Karim Rashid , Zaksas Victoria , Kim JangKeun , I Anu , Muratani Masafumi , Tasoula Alexia , Singh Ruth , Chen Yen-Kai , Overbey Eliah , Park Jiwoon , Povero Davide , Borg Joseph , Klotz Remi , Yu Min , Young Steven , Mason Christopher , Szewczyk Nathaniel , Clair Riley St , Karouia Fathi , Beheshti Afshin 
Published: January 2023

Abstract

Organismal adaptations to spaceflight have been characterized at the molecular level in model organisms, including Drosophila and C. elegans. Here we extend molecular work to energy metabolism and sex hormone signaling in mice and humans. We found spaceflight-induced changes in insulin and estrogen signaling in rodents and humans. Murine changes were most prominent in the liver, where we observed inhibition of insulin and estrogen receptor signaling with concomitant hepatic insulin resistance and steatosis. Based on the metabolic demand, metabolic pathways mediated by insulin and estrogen vary among muscles, specifically between the soleus and extensor digitorum longus. In humans, spaceflight induced changes in insulin and estrogen related genes and pathways. Pathway analysis demonstrated spaceflight-induced changes in insulin resistance, estrogen signaling, stress response, and viral infection. These data strongly suggest the need for further research on the metabolic and reproductive endocrinologic effects of space travel, if we are to become a successful interplanetary species
Neurogenetic Mechanisms of Risk for ADHD: Examining Associations of Functionally-Annotated Polygenic Scores and Brain Volumes in a Population Cohort
Authors: He Quanfa , Keding Taylor , Zhang Qi , Miao Jiacheng , Herringa Ryan J. , Lu Qiongshi , Travers Brittany G. , Li James J. 
Published: January 2023

Abstract

Background:ADHD polygenic scores (PGS) are reliably predictive of ADHD outcomes across studies. However, traditional PGS arestatisticalindices of genetic liability – predictive of ADHD but uninformed bybiologicalinformation. The objective of our study was to determine whether a novel, biologically-informed, functionally annotated ADHD PGS can reveal specific brain mechanisms of risk for ADHD.Methods:Data were from the Philadelphia Neurodevelopmental Cohort (n=555). Multiple mediation models were tested to examine the indirect effects of ADHD PGS (including one using a functionally annotated approach, i.e.,AnnoPred) on ADHD inattention (IA) and hyperactivity-impulsivity (HI) symptoms, via gray matter volumes in the cingulate gyrus, angular gyrus, caudate, dorsolateral prefrontal cortex (DLPFC), and inferior temporal lobe. Age-stratified analyses (children, adolescents, and young adults) were also conducted to account for developmental differences.Results:A direct effect was detected betweenAnnoPredPGS and IA symptoms in adolescents only. No indirect effects via brain volumes were detected for either IA or HI symptoms. However, ADHD PGS were consistently associated with the DLPFC.Conclusions:A biologically-informed PGS yielded a more powerful (and developmentally-specific) signal in detecting a direct effect of ADHD PGS on adolescent IA symptoms. However, no indirect effects between ADHD PGS and ADHD symptomology via the brain emerged. ADHD will become increasing predictive as discovery sample sizes climb. Studies that integrate both genetic and neuroimaging information are critical to advancing our understanding of the biological mechanisms underlying ADHD.
Evaluation of the removal of nutrients coupling of the cultivation of Spirulina platensis with piggery wastewater
Authors: liang changli , Zhang Nali , Pang Yu , Li Siyuan , Shang Jiafan , Zhang Yipeng , Kuang Zhenzhong , Liu Junhe , Fei Hua 
Published: January 2023

Abstract
The discharge of livestock wastewater without treatment in depth severely harm to the environment and human health, cultivation microalgae as feedstock for biodiesel and animal feed additive using livestock wastewater coupling of the treatment of wastewater has become a research topic. In this study, the cultivation conditions of Spirulina platensis using piggery wastewater were optimized by response surface method, and the removal of nutrients from the piggery wastewater coupling of Spirulina platensis as the feedstock for animal feed additive was evaluation. The results of single factor experiments confirmed cupric ion seriously inhibited the growth of Spirulina platensis, while nitrogen, phosphorous and zinc all low promotes high inhabitation. Spirulina platensis grew well in the dilution piggery wastewater supplemented with moderate sodium bicarbonate, which indicated it is the limited nutrients. The OD560 of S. platensi reached 1.83 at the optimal conditions proposed by response surface method as follows: piggery wastewater was diluted to 4 times, 7 g/L sodium bicarbonate, pH 10.5, initial OD560 0.63, light intensity 3030 lux, light/dark 16/8 and cultured time 8 days. Spirulina platensis cultured in the diluted piggery wastewater contained 43.75% protein, 9.4% crude lipid, 8.826 mg/g chlorophyll, 4.18% total sugar, 27.7 mg/kg Cu and 246.2 mg/kg Zn. The analysis of the chemical compositions of Spirulina platensis indicated it can as the feedstock for animal feed additive. The removal efficiency of TN, TP, COD, Zn and Cu from the wastewater was 76%, 72%, 93.1%, 93.5% and 82.5%, respectively. These results demonstrated the removal of nutrients from piggery wastewater coupling culturing S. platensi as the feedstock for animal feed additive is feasible.
Prevalence of, Association with, Severity of, and Prognostic Role of Serum Hemoglobin Level in Acutely Decompensated Heart Failure Patients
Authors: Omoomi Sepehr , Heidarpour Maryam , Rabanipour Najmeh , Saadati Mona , Vakilbashi Omid , Shafie Davood 
Published: January 2023

Abstract
Background
The role of serum hemoglobin level (SHL) in the short prognosis of heart failure (HF) remains unclear. We aimed to declare the prevalence of, association with, severity of, and prognostic role of SHL with HF.
Methods
Using the data from the PROVE-HF study, we assessed the association between anemia and polycythemia (Hb < 13g/Lit, > 16.5g/Lit in males and < 12g/Lit, and > 16g/Lit in females, respectively) and short-term mortality using Cox proportional hazard modeling, with adjustment of clinically relevant variables.
Results
Of 3652 HF patients, 48.40% including 1546 (42.33%), 118 (3.23%), 9 (0.24%), and 422 (11.55%) mild, moderate, severely anemic, and polycythemic found, respectively. Compared to non-anemic patients, anemic patients were mainly male, older, and were more likely to have diabetes mellitus (DM), hypertension (HTN), kidney, and thyroid disease. Important predictors of short-term mortality were lower systolic and diastolic blood pressure, lower SHL, and higher blood urea nitrogen (BUN). Anemic patients had higher all-cause mortality [adjusted hazard ratio (aHR) 1.213, 95% confidence interval [CI] 1.054–1.396]. Moderate anemia increased the risk of mortality by approximately 80% in males (aHR 1.793, 95% CI 1.308–2.458) and females (aHR 1.790, 95% CI 1.312–2.442), respectively. Polycythemia had no association with short-term mortality in both genders (P-value > 0.05).
Conclusions
This study revealed that anemia is an adverse prognostic factor for short-term mortality in HF patients, with a greater risk of mortality in moderately anemic patients.
Prevalence and associated health and lifestyle factors of myopic maculopathy in northern china: the Kailuan Eye Study
Authors: Wang Haiwei , Zhou Jinqiong , Wang Ya Xing , Wang Qian , Yan Yanni , Yang Xuan , Yang Jingyan , Zhou Wenjia , Nie Zihan , Shi Xuhan , Ji Haixia , Zhang Yongpeng , Wei Wenbin , Shi Xuehui 
Published: January 2023

Abstract
Background
To evaluate the prevalence and associated health and lifestyle factors of myopic maculopathy (MM) in a northern Chinese industrial city.
Methods
The cross-sectional Kailuan Eye Study included subjects who participated in the longitudinal Kailuan Study in 2016. Ophthalmologic and general examinations were performed on all the participants. MM was graded based on fundus photographs using the International Photographic Classification and Grading System. The prevalence of MM was evaluated. Univariate and multiple logistic regression were adopted to evaluated risk factors of MM.
Results
The study included 8330 participants with gradable fundus photographs for MM and ocular biometry data. The prevalence of MM was 1.11% (93/8330; 95% confidence interval [CI] 0.89–1.33%). Diffuse chorioretinal atrophy, patchy chorioretinal atrophy, macular atrophy, and plus lesions were observed in 72 (0.9%), 15 (0.2%), 6 (0.007%), and 32 eyes (0.4%), respectively. MM was more common in eyes with longer axial length (OR 4.517; 95%CI 3.273 to 6.235) and in participants with hypertension (OR 3.460; 95%CI 1.152 to 10.391), and older age (OR 1.084; 95%CI 1.036 to 1.134).
Conclusions
The MM was present in 1.11% of the northern Chinese individuals 21 years or older and the associate factors include longer axial length, older age, and hypertension.