bluuebunny/crossref_metadata_2025_split huggingface dataset:
Recent Advances on Cell Culture Platforms for In Vitro Drug Screening and Cell Therapies: From Conventional to Microfluidic Strategies
Authors: Cardoso Beatriz D. , Castanheira Elisabete M. S. , Lanceros‐Méndez Senentxu , Cardoso Vanessa F. 
Published: March 2023

AbstractThe clinical translations of drugs and nanomedicines depend on coherent pharmaceutical research based on biologically accurate screening approaches. Since establishing the 2D in vitro cell culture method, the scientific community has improved cell‐based drug screening assays and models. Those advances result in more informative biochemical assays and the development of 3D multicellular models to describe the biological complexity better and enhance the simulation of the in vivo microenvironment. Despite the overall dominance of conventional 2D and 3D cell macroscopic culture methods, they present physicochemical and operational challenges that impair the scale‐up of drug screening by not allowing a high parallelization, multidrug combination, and high‐throughput screening. Their combination and complementarity with microfluidic platforms enable the development of microfluidics‐based cell culture platforms with unequivocal advantages in drug screening and cell therapies. Thus, this review presents an updated and consolidated view of cell culture miniaturization's physical, chemical, and operational considerations in the pharmaceutical research scenario. It clarifies advances in the field using gradient‐based microfluidics, droplet‐based microfluidics, printed‐based microfluidics, digital‐based microfluidics, SlipChip, and paper‐based microfluidics. Finally, it presents a comparative analysis of the performance of cell‐based methods in life research and development to achieve increased precision in the drug screening process.
Enhanced Superconductivity and Upper Critical Field in Ta‐Doped Weyl Semimetal Td‐MoTe2
Authors: Zhang Yong , Fei Fucong , Liu Ruxin , Zhu Tongshuai , Chen Bo , Qiu Tianyu , Zuo Zewen , Guo Jingwen , Tang Wenchao , Zhou Lifan , Xi Xiaoxiang , Wu Xiaoshan , Wu Di , Zhong Zhicheng , Song Fengqi , Zhang Rong , Wang Xuefeng 
Published: March 2023

Abstract2D transition metal dichalcogenides are promising platforms for next‐generation electronics and spintronics. The layered Weyl semimetal (W,Mo)Te2 series features structural phase transition, nonsaturated magnetoresistance, superconductivity, and exotic topological physics. However, the superconducting critical temperature of the bulk (W,Mo)Te2 remains ultralow without applying a high pressure. Here, the significantly enhanced superconductivity is observed with a transition temperature as large as about 7.5 K in bulk Mo1−xTaxTe2 single crystals upon Ta doping (0 ≤ x ≤ 0.22), which is attributed to an enrichment of density of states at the Fermi level. In addition, an enhanced perpendicular upper critical field of 14.5 T exceeding the Pauli limit is also observed in Td‐phase Mo1−xTaxTe2 (x = 0.08), indicating the possible emergence of unconventional mixed singlet–triplet superconductivity owing to the inversion symmetry breaking. This work provides a new pathway for exploring the exotic superconductivity and topological physics in transition metal dichalcogenides.
Bioinspired Processing: Complex Coacervates as Versatile Inks for 3D Bioprinting
Authors: Khoonkari Mohammad , Es Sayed Julien , Oggioni Marta , Amirsadeghi Armin , Dijkstra Peter , Parisi Daniele , Kruyt Frank , van Rijn Patrick , Włodarczyk‐Biegun Małgorzata Katarzyna , Kamperman Marleen 
Published: March 2023

Abstract3D bioprinting is a powerful fabrication technique in biomedical engineering, which is currently limited by the number of available materials that meet all physicochemical and cytocompatibility requirements for biomaterial inks. Inspired by the key role of coacervation in the extrusion and spinning of many natural materials, hyaluronic acid–chitosan complex coacervates are proposed here as tunable biomaterial inks. Complex coacervates are obtained through an associative liquid–liquid phase separation driven by electrostatic attraction between oppositely charged macromolecules. They offer bioactive properties and facile modulation of their mechanical properties through mild physicochemical changes in the environment, making them attractive for 3D bioprinting. Fine‐tuning the salt concentration, pH, and molecular weight of the constituent polymers results in biomaterial inks that are printable in air and water. The biomaterial ink, initially a viscoelastic fluid, transitions into a viscoelastic solid upon printing due to dehydration (for printing in air) or due to a change in pH and ionic composition (for printing in solution). Consequently, scaffolds printed using the complex coacervate inks are stable without the need for post‐printing processing. Fabricated cell culture scaffolds are cytocompatible and show long‐term topological stability. These results pave the way to a new class of easy‐to‐handle tunable biomaterials for biofabrication.
Chemotaxis‐guided Self‐propelled Macrophage Motor for Targeted Treatment of Acute Pneumonia
Authors: Yue Ludan , Gao Cheng , Li Junyan , Chen Hanbin , Lee Simon M. Y. , Luo Ruifeng , Wang Ruibing 
Published: March 2023

AbstractImmune cells exhibit great potential as carriers of nanomedicine, attributed to their high tolerance to internalized nanomaterials and targeted accumulation in inflammatory tissues. However, the premature efflux of internalized nanomedicine during systemic delivery and slow infiltration into inflammatory tissues have limited their translational applications. Herein, a motorized cell platform as a nanomedicine carrier for highly efficient accumulation and infiltration in the inflammatory lungs and effective treatment of acute pneumonia are reported. β‐Cyclodextrin and adamantane respectively modified manganese dioxide nanoparticles are intracellularly self‐assembled into large aggregates mediated via host‐guest interactions, to effectively inhibit the efflux of nanoparticles, catalytically consume/deplete H2O2 to alleviate inflammation, and generate O2 to propel macrophage movement for rapid tissue infiltration. With curcumin loaded into MnO2 nanoparticles, macrophages carry the intracellular nano‐assemblies rapidly into the inflammatory lungs via chemotaxis‐guided, self‐propelled movement, for effective treatment of acute pneumonia via immunoregulation induced by curcumin and the aggregates.
Photoelectrochemical N2‐to‐NH3 Fixation with High Efficiency and Rates via Optimized Si‐Based System at Positive Potential versus Li0/+
Authors: Zhang Xiaoran , Lyu Yanhong , Zhou Huaijuan , Zheng Jianyun , Huang Aibin , Ding Jingjing , Xie Chao , De Marco Roland , Tsud Nataliya , Kalinovych Viacheslav , Jiang San Ping , Dai Liming , Wang Shuangyin 
Published: March 2023

AbstractAs a widely used commodity chemical, ammonia is critical for producing nitrogen‐containing fertilizers and serving as the promising zero‐carbon energy carrier. Photoelectrochemical nitrogen reduction reaction (PEC NRR) can provide a solar‐powered green and sustainable route for synthesis of ammonia (NH3). Herein, an optimum PEC system is reported with an Si‐based hierarchically‐structured PdCu/TiO2/Si photocathode and well‐thought‐out trifluoroethanol as the proton source for lithium‐mediated PEC NRR, achieving a record high NH3 yield of 43.09 µg cm−2 h−1 and an excellent faradaic efficiency of 46.15% under 0.12 MPa O2 and 3.88 MPa N2 at 0.07 V versus lithium(0/+) redox couple (vs Li0/+). PEC measurements coupled with operando characterization reveal that the PdCu/TiO2/Si photocathode under N2 pressures facilitate the reduction of N2 to form lithium nitride (Li3N), which reacts with active protons to produce NH3 while releasing the Li+ to reinitiate the cycle of the PEC NRR. The Li‐mediated PEC NRR process is further enhanced by introducing small amount of O2 or CO2 under pressure by accelerating the decomposition of Li3N. For the first time, this work provides mechanistic understanding of the lithium‐mediated PEC NRR process and opens new avenues for efficient solar‐powered green conversion of N2‐to‐NH3.
Density of States Engineering of n‐Doped Conjugated Polymers for High Charge Transport Performances
Authors: Wang Xin‐Yi , Yu Zi‐Di , Lu Yang , Yao Ze‐Fan , Zhou Yang‐Yang , Pan Chen‐Kai , Liu Yi , Wang Zi‐Yuan , Ding Yi‐Fan , Wang Jie‐Yu , Pei Jian 
Published: March 2023

AbstractCharge transport of conjugated polymers in functional devices closely relates to their density of states (DOS) distributions. However, systemic DOS engineering for conjugated polymers is challenging due to the lack of modulated methods and the unclear relationship between DOS and electrical properties. Here, the DOS distribution of conjugated polymers is engineered to enhance their electrical performances. The DOS distributions of polymer films are tailored using three processing solvents with different Hansen solubility parameters. The highest n‐type electrical conductivity (39 ± 3 S cm−1), the highest power factor (63 ± 11 µW m−1 K−2), and the highest Hall mobility (0.14 ± 0.02 cm2 V−1 s−1) of the polymer (FBDPPV‐OEG) are obtained in three films with three various DOS distributions, respectively. Through theoretical and experimental exploration, it is revealed that the carrier concentration and transport property of conjugated polymers can be efficiently controlled by DOS engineering, paving the way for rationally fabricating organic semiconductors.
Advances of NIR Light Responsive Materials for Diagnosis and Treatment of Brain Diseases
Authors: Xue Dongzhi , Wang Yinghui , Zhang Hongjie 
Published: March 2023

AbstractBrain diseases pose a great threat to human health worldwide, thus it is of great importance to explore new materials for their diagnosis and therapy. Compared with the ultraviolet (UV) and visible light, near infrared (NIR) light has better biosafety, lower tissue auto‐fluorescence, and stronger penetration into skull. Hence, NIR light responsive materials have attracted great attention recently which can not only realize the high spatial resolution and signal‐to‐noise ratio imaging for diagnosis, but also achieve effective NIR light triggered treatment noninvasively. Besides, the brain lesions can be visualized clearly by NIR imaging, which can provide more accurate information for guiding treatments. Therefore, this work systematically summarizes the recent advances of NIR light responsive materials for diagnosis and treatment of brain diseases. Firstly, this work briefly reviews the pathological features of blood brain barrier (BBB) and the rational design of materials to target brain disease. Then, the emerging approaches for diagnosis and treatment of various brain diseases with NIR light responsive materials are introduced. Subsequently, the imaging‐guided therapies are elaborated, such as the fluorescence imaging guided surgery and sonodynamic therapy. Finally, some challenges and prospects on the design of NIR light responsive materials for precise diagnosis and therapy of brain diseases are put forward.
GPR176 Promotes Cancer Progression by Interacting with G Protein GNAS to Restrain Cell Mitophagy in Colorectal Cancer
Authors: Tang Junwei , Peng Wen , Ji Jiangzhou , Peng Chaofan , Wang Tuo , Yang Peng , Gu Ji'ou , Feng Yifei , Jin Kangpeng , Wang Xiaowei , Sun Yueming 
Published: March 2023

AbstractGPR176 belongs to the G protein‐coupled receptor superfamily, which responds to external stimuli and regulates cancer progression, but its role in colorectal cancer (CRC) remains unclear. In the present study, expression analyses of GPR176 are performed in patients with colorectal cancer. Genetic mouse models of CRC coupled with Gpr176‐deficiency are investigated, and in vivo and in vitro treatments are conducted. A positive correlation between GPR176 upregulation and the proliferation and poor overall survival of CRC is demonstrated. GPR176 is confirmed to activate the cAMP/PKA signaling pathway and modulate mitophagy, promoting CRC oncogenesis and development. Mechanistically, the G protein GNAS is recruited intracellularly to transduce and amplify extracellular signals from GPR176. A homolog model tool confirmed that GPR176 recruits GNAS intracellularly via its transmembrane helix 3‐intracellular loop 2 domain. The GPR176/GNAS complex inhibits mitophagy via the cAMP/PKA/BNIP3L axis, thereby promoting the tumorigenesis and progression of CRC.
Nanostructured 3D‐Printed Hybrid Scaffold Accelerates Bone Regeneration by Photointegrating Nanohydroxyapatite
Authors: Tong Lei , Pu Xiaocong , Liu Quanying , Li Xing , Chen Manyu , Wang Peilei , Zou Yaping , Lu Gonggong , Liang Jie , Fan Yujiang , Zhang Xingdong , Sun Yong 
Published: March 2023

AbstractNanostructured biomaterials that replicate natural bone architecture are expected to facilitate bone regeneration. Here, nanohydroxyapatite (nHAp) with vinyl surface modification is acquired by silicon‐based coupling agent and photointegrated with methacrylic anhydride‐modified gelatin to manufacture a chemically integrated 3D‐printed hybrid bone scaffold (75.6 wt% solid content). This nanostructured procedure significantly increases its storage modulus by 19.43‐fold (79.2 kPa) to construct a more stable mechanical structure. Furthermore, biofunctional hydrogel with biomimetic extracellular matrix is anchored onto the filament of 3D‐printed hybrid scaffold (HGel‐g‐nHAp) by polyphenol‐mediated multiple chemical reactions, which contributes to initiate early osteogenesis and angiogenesis by recruiting endogenous stem cells in situ. Significant ectopic mineral deposition is also observed in subcutaneously implanted nude mice with storage modulus enhancement of 25.3‐fold after 30 days. Meanwhile, HGel‐g‐nHAp realizes substantial bone reconstruction in the rabbit cranial defect model, achieving 61.3% breaking load strength and 73.1% bone volume fractions in comparison to natural cranium 15 weeks after implantation. This optical integration strategy of vinyl modified nHAp provides a prospective structural design for regenerative 3D‐printed bone scaffold.
Geographical variation of the structure and population parameters of the freshwater prawnMacrobrachium macrobrachion(Herklots, 1851) in West African coastal hydrosystems: Insights from Benin
Authors: Koussovi Guillaume , Niass Farokh , Ahouansou Montcho Simon , Bonou Clément Agossou , Montchowui Elie 
Published: March 2023

AbstractThe freshwater prawnMacrobrachium macrobrachion(Herklots, 1851) is widely exploited in the Oueme Delta and the low Mono in Benin. The structure and population parameters ofM. macrobrachionwere evaluated to provide accurate scientific information for the better exploitation and rational management of freshwater prawn species in these areas. In Oueme Delta (1928 specimens) and low Mono (1045 specimens), 2973 specimens were sampled by artisanal and experimental fishing and analysed. The prawns were caught between January and December 2016. Total length and total weight were taken from each specimen. Global and monthly size–frequency distributions showed that recruitment in both populations takes place from August to October with a peak in September. The population parameters obtained by analysing frequency data with FiSAT II software (FAO‐ICLARM) showed anL∞ = 15.50 cm,K = 0.35 year−1,t0 = −0.56 year in Oueme Delta andL∞ = 15.30 cm,K = 0.45 year−1,t0 = −0.43 year in low Mono. The growth performance index (Φ′) was 1.93 and 2.02 for the Oueme Delta and low Mono populations, respectively. The exploitation rates (EmaxandE0.5) suggest that more than half of theM. macrobrachionpopulation was exploited in the two areas. Natural and fishing mortality rates showed that both populations ofM. macrobrachionwere vulnerable to natural and fishing death. The estimated first capture size (Lc) revealed that the majority of the two populations ofM. macrobrachionwere captured before they reached the size at first sexual maturity (L50), reflecting fishing pressure on small individuals and growth overfishing in both areas. These results showed that the used fishing gears and the fishing frequencies in both areas did not allow the majority of the prawn to grow and reproduce at least once before being caught.
African American mothers' perceptions of infant body size are prospectively associated with infant growth and BMI
Authors: Rothman Joshua , Shirley Meghan K. , Parks Elizabeth Prout , Wu Gary D. , Kelly Andrea , Ford Eileen , Zemel Babette S. 
Published: March 2023

AbstractObjectivesThe effect of mothers' perceptions of infant body size on infant growth and later BMI is poorly understood. We aimed to assess whether maternal perceptions were associated with infant BMI and weight gain and to identify factors that may influence maternal perceptions.MethodsWe analyzed data from a prospective, longitudinal study of pregnant African American women living with healthy weight (BMI < 25 kg/m2) or obesity (BMI ≥ 30 kg/m2). We collected sociodemographic, feeding mode, perceived stress, depression, and food insecurity information. The African American Infant Body Habitus Scale assessed maternal perceptions of infant body size at age 6 months. A “maternal satisfaction with infant body size” score was derived. Infant BMI z‐scores (BMIZ) were calculated at 6 and 24 months.ResultsMaternal perception and satisfaction scores did not differ between obese (n = 148) and healthy weight (n = 132) groups. Perception of infant size at 6 months was positively associated with infant BMIZ at 6 and 24 months. A positive association of maternal satisfaction scores with change in infant BMIZ from 6 to 24 months indicated that BMIZ changed less for infants whose mothers preferred them to be smaller at 6 months. Perception and satisfaction scores were not associated with feeding variables, maternal stress, depression, socioeconomic status, or food security status.ConclusionMothers' perceptions of and satisfaction with infant size correlated with current and later infant BMI. However, mother's perceptions were not associated with maternal weight status or other factors explored for their potential to impact maternal perceptions. Further work is needed to elucidate factors linking maternal perception/satisfaction and infant growth.
PAK1 c.1409 T > a (p. Leu470Gln) de novo variant affects the protein kinase domain, leading to epilepsy, macrocephaly, spastic quadriplegia, and hydrocephalus: Case report and review of the literature
Authors: Corriveau Melina L. , Amaya Sabrina I. , Koebel Mary Clare , Lerma Vanesa C. , Michener Sydney L. , Turner Alicia , Schultz Rebecca J. , Seto Elaine S. , Diaz‐Medina Gloria E. , Craigen William J. , Swann John W. , Xue Mingshan , Chao Hsiao‐Tuan 
Published: March 2023

AbstractThe p‐21‐activated kinase 1 (PAK1) protein, encoded by the PAK1 gene, is an evolutionarily conserved serine/threonine‐protein kinase that regulates key cellular developmental processes. To date, seven de novo PAK1 variants have been reported to cause the Intellectual Developmental Disorder with Macrocephaly, Seizures, and Speech Delay (IDDMSSD). In addition to the namesake features, other common characteristics include structural brain anomalies, delayed development, hypotonia, and dysmorphic features. Here, we report a de novo PAK1 NM_002576.5: c.1409 T > A variant (p.Leu470Gln) identified by trio genome sequencing (GS) in a 13‐year‐old boy with postnatal macrocephaly, obstructive hydrocephalus, medically refractory epilepsy, spastic quadriplegia, white matter hyperintensities, profound developmental disabilities, and a horseshoe kidney. This is the first recurrently affected residue identified in the protein kinase domain. Combined assessment of the eight pathogenic PAK1 missense variants reveal that the variants cluster in either the protein kinase or autoregulatory domains. Although interpretation of the phenotypic spectrum is limited by the sample size, neuroanatomical alterations were found more often in individuals with PAK1 variants in the autoregulatory domain. In contrast, non‐neurological comorbidities were found more often in individuals with PAK1 variants in the protein kinase domain. Together, these findings expand the clinical spectrum of PAK1‐associated IDDMSSD and reveal potential correlations with the affected protein domains.
Proteomics and metabolomics analysis of nasal lavage fluid in chronic rhinosinusitis with nasal polyps
Authors: Yang Yujuan , Guo Jing , Yao Yao , Wang Jianwei , Yin Jiali , Guo Ying , Wang Cai , Zhang Yu , Song Xicheng 
Published: March 2023

Key Points
An integrated proteomics and metabolomics were used to investigate the pathogenesis of CRSwNP.
Amino acid metabolism and mitochondrial dysfunction play key roles in the pathogenesis of CRSwNP.
Impact of low‐value medications on quality of life, hospitalization and costs – A longitudinal analysis of patients living with dementia
Authors: Platen Moritz , Flessa Steffen , Teipel Stefan , Rädke Anika , Scharf Annelie , Mohr Wiebke , Buchholz Maresa , Hoffmann Wolfgang , Michalowsky Bernhard 
Published: March 2023

AbstractIntroductionThis study aimed to analyze the impact of low‐value medications (Lvm), that is, medications unlikely to benefit patients but to cause harm, on patient‐centered outcomes over 24 months.MethodsThis longitudinal analysis was based on baseline, 12 and 24 months follow‐up data of 352 patients with dementia. The impact of Lvm on health‐related quality of life (HRQoL), hospitalizations, and health care costs were assessed using multiple panel‐specific regression models.ResultsOver 24 months, 182 patients (52%) received Lvm at least once and 56 (16%) continuously. Lvm significantly increased the risk of hospitalization by 49% (odds ratio, confidence interval [CI] 95% 1.06–2.09; p = 0.022), increased health care costs by €6810 (CI 95% −707€–14,27€; p = 0.076), and reduced patients' HRQoL (b = −1.55; CI 95% −2.76 to −0.35; p = 0.011).DiscussionMore than every second patient received Lvm, negatively impacting patient‐reported HRQoL, hospitalizations, and costs. Innovative approaches are needed to encourage prescribers to avoid and replace Lvm in dementia care.Highlights
Over 24 months, more than every second patient received low‐value medications (Lvm).
Lvm negatively impact physical, psychological, and financial outcomes.
Appropriate measures are needed to change prescription behaviors.
The association between COVID‐19 and cognitive performance: A Mendelian randomization analysis
Authors: Ran Shu , Yao Jia , Liu Baolin 
Published: March 2023

AbstractIntroductionPeople with COVID‐19 had poorer general cognitive functioning compared to people without COVID‐19. The causal link between COVID‐19 and cognitive impairment is still unknown.MethodsMendelian randomization (MR) is a statistical approach based on genome‐wide association studies (GWAS) to construct instrumental variables (IVs) and can effectively bring down the confounding bias of environmental or other disease factors, because alleles are randomly assigned to offspring.ResultsThere was consistent evidence that cognitive performance was causally associated with COVID‐19; this suggests that people with better cognitive performance are less likely to be infected with COVID‐19. The reverse MR analysis treating COVID‐19 as the exposure and cognitive performance as the outcome demonstrated an insignificant association, indicating the unidirectionality of the relationship.DiscussionOur study provided credible evidence that cognitive performance has an impact on COVID‐19. Future research should focus on long‐term impact of cognitive performance on COVID‐19.