bluuebunny/crossref_metadata_2025_split huggingface dataset:
Aldehyde Dehydrogenase, a Marker of Normal and Malignant Stem Cells, Typifies Mesenchymal Progenitors in Perivascular Niches
Authors: Gomez-Salazar Mario A , Wang Yiyun , Thottappillil Neelima , Hardy Reef W , Alexandre Manon , Höller Fabian , Martin Niall , Gonzalez-Galofre Zaniah N , Stefancova Dorota , Medici Daniele , James Aaron W , Péault Bruno 
Published: June 2023

Abstract
Innate mesenchymal stem cells exhibiting multilineage differentiation and tissue (re)generative—or pathogenic—properties reside in perivascular niches. Subsets of these progenitors are committed to either osteo-, adipo-, or fibrogenesis, suggesting the existence of a developmental organization in blood vessel walls. We evaluated herein the activity of aldehyde dehydrogenase, a family of enzymes catalyzing the oxidation of aldehydes into carboxylic acids and a reported biomarker of normal and malignant stem cells, within human adipose tissue perivascular areas. A progression of ALDHLow to ALDHHigh CD34+ cells was identified in the tunica adventitia. Mesenchymal stem cell potential was confined to ALDHHigh cells, as assessed by proliferation and multilineage differentiation in vitro of cells sorted by flow cytometry with a fluorescent ALDH substrate. RNA sequencing confirmed and validated that ALDHHigh cells have a progenitor cell phenotype and provided evidence that the main isoform in this fraction is ALDH1A1, which was confirmed by immunohistochemistry. This demonstrates that ALDH activity, which marks hematopoietic progenitors and stem cells in diverse malignant tumors, also typifies native, blood vessel resident mesenchymal stem cells.
Comprehensive analysis of circRNAs for N7‐methylguanosine methylation modification in human oral squamous cell carcinoma
Authors: Sun Dongyuan , Song Ning , Li Minmin , Chen Xi , Zhang Xinyue , Yu Yang , Ying Jicheng , Xu Mengqi , Zheng Wentian , Han Chengbing , Ji Honghai , Jiang Yingying 
Published: June 2023

AbstractN7‐methylguanosine (m7G) modification is closely related to the occurrence of tumors. However, the m7G modification of circRNAs in oral squamous cell carcinoma (OSCC) remains to be investigated. Methylated RNA immunoprecipitation sequencing (MeRIP‐seq) was used to measure the methylation levels of m7G and identify m7G sites in circRNAs in human OSCC and normal tissues. The host genes of differentially methylated and differentially expressed circRNAs were analyzed by Gene Ontology (GO) enrichment and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses, and circRNA–miRNA–mRNA networks were predicted using the miRanda and miRDB databases. The analysis identified 2348 m7G peaks in 624 circRNAs in OSCC tissues. In addition, the source of m7G‐methylated circRNAs in OSCC was mainly the sense overlap region compared with normal tissues. The most conserved m7G motif in OSCC tissues was CCUGU, whereas the most conserved motif in normal tissues was RCCUG (R = G/A). Importantly, GO enrichment and KEGG pathway analysis showed that the host genes of differentially methylated and differentially expressed circRNAs were involved in many cellular biological functions. Furthermore, the significantly differentially expressed circRNAs were analyzed to predict the circRNA–miRNA–mRNA networks. This study revealed the whole profile of circRNAs of differential m7G methylation in OSCC and suggests that m7G‐modified circRNAs may impact the development of OSCC.
Implications of left atrial volume index in patients with three-vessel coronary disease: A 6.6-year follow-up cohort study
Authors: Liu Ru , Song Lei , Zhang Ce , Jiang Lin , Tian Jian , Xu Lianjun , Feng Xinxing , Wan Linyuan , Zhao Xueyan , Xu Ou , Li Chongjian , Gao Runlin , Hui Rutai , Zhao Wei , Yuan Jinqing 
Published: June 2023

Abstract

Background:
Risk assessment and treatment stratification for three-vessel coronary disease (TVD) remain challenging. This study aimed to investigate the prognostic value of left atrial volume index (LAVI) with the Synergy Between Percutaneous Coronary Intervention with Taxus and Cardiac Surgery (SYNTAX) score II, and its association with the long-term prognosis after three strategies (percutaneous coronary intervention [PCI], coronary artery bypass grafting [CABG], and medical therapy [MT]) in patients with TVD.

Methods:
This study was a post hoc analysis of a large, prospective cohort of patients with TVD in China, that aimed to determine the long-term outcomes after PCI, CABG, or optimal MT alone. A total of 8943 patients with TVD were consecutively enrolled between 2004 and 2011 at Fuwai Hospital. A total of 7818 patients with available baseline LAVI data were included in the study. Baseline, procedural, and follow-up data were collected. The primary endpoint was major adverse cardiac and cerebrovascular events (MACCE), which was a composite of all-cause death, myocardial infarction (MI), and stroke. Secondary endpoints included all-cause death, cardiac death, MI, revascularization, and stroke. Long-term outcomes were evaluated among LAVI quartile groups.

Results:
During a median follow-up of 6.6 years, a higher LAVI was strongly associated with increased risk of MACCE (Q3: hazard ratio [HR] 1.20, 95% confidence interval [CI] 1.06–1.37, P = 0.005; Q4: HR 1.85, 95%CI 1.64–2.09, P <0.001), all-cause death (Q3: HR 1.41, 95% CI 1.17–1.69, P <0.001; Q4: HR 2.54, 95%CI 2.16–3.00, P <0.001), and cardiac death (Q3: HR 1.81, 95% CI 1.39–2.37, P <0.001; Q4: HR 3.47, 95%CI 2.71–4.43, P <0.001). Moreover, LAVI significantly improved discrimination and reclassification of the SYNTAX score II. Notably, there was a significant interaction between LAVI quartiles and treatment strategies for MACCE. CABG was associated with lower risk of MACCE than MT alone, regardless of LAVI quartiles. Among patients in the fourth quartile, PCI was associated with significantly increased risk of cardiac death compared with CABG (HR: 5.25, 95% CI: 1.97–14.03, P = 0.001).

Conclusions:
LAVI is a potential index for risk stratification and therapeutic decision-making in patients with three-vessel coronary disease. CABG is associated with improved long-term outcomes compared with MT alone, regardless of LAVI quartiles. When LAVI is severely elevated, PCI is associated with higher risk of cardiac death than CABG.
Prognostic Implications of Lateral Lymph Nodes in Rectal Cancer: A Population-Based Cross-Sectional Study with Standardized Radiological Evaluation after Dedicated Training
Authors: Sluckin Tania C. , van Geffen Eline G.M. , Hazen Sanne-Marije J.A. , Horsthuis Karin , Beets-Tan Regina G.H. , Marijnen Corrie A.M. , Tanis Pieter J. , Kusters Miranda 
Published: June 2023

BACKGROUND:
There is an ongoing discussion regarding the prognostic implications of the presence, short-axis diameter and location of lateral lymph nodes.

OBJECTIVE:
To analyze lateral lymph node characteristics, the role of downsizing on restaging MRI and associated local recurrence rates for patients with cT3-4 rectal cancer after MRI re-review and training.

DESIGN:
Retrospective population-based cross-sectional study.

SETTINGS:
This collaborative project was led by local investigators from surgery and radiology departments in 60 Dutch hospitals.

PATIENTS:
A total of 3057 patients underwent rectal cancer surgery in 2016: 1109 had cT3-4 tumor located ≤8 cm from the anorectal junction of which 890 received neoadjuvant therapy.

MAIN OUTCOME MEASURES:
Local recurrence and ipsilateral local recurrence rates.

RESULTS:
Re-review identified 314 patients (35%) with visible lateral lymph nodes. 30 of these patients had either only long-stretched obturator (n = 13) or external iliac (n = 17) nodes and both did not lead to any lateral local recurrences. The presence of internal iliac/obturator lateral lymph nodes (n = 284) resulted in 4-year local recurrence and lateral local recurrence rates of 16.4% and 8.8%, respectively. Enlarged (≥7 mm) lateral lymph nodes (n = 122) resulted in higher 4-year local recurrence (20.8%, 13.1%, 0%, p < .001) and lateral local recurrence (14.7%, 4.4%, 0%, p < 0.001) rates compared to smaller and no lateral lymph nodes, respectively. Visible lateral lymph nodes (hazard ratio 1.8 [1.1-2.8]) and enlarged lateral lymph nodes (hazard ratio 1.9 [1.1-3.5]) were independently associated with local recurrence in multivariable analysis. Enlarged lateral lymph nodes with malignant features had higher 4-year LLR rates of 17.0%. Downsizing had no impact on lateral local recurrence rates. Enlarged lateral lymph nodes were associated with higher univariate 4-year distant metastasis rates (36.4% 24.4%, p = 0.021), but not in multivariable analysis (hazard ratio 1.3 [0.9-1.]), and did not worsen overall survival.

LIMITATIONS:
This study was limited by the retrospective design and total number of patients with lateral lymph nodes.

CONCLUSIONS:
The risk of lateral local recurrence due to (enlarged) lateral lymph nodes was confirmed, but without prognostic impact of downsizing after neoadjuvant therapy. These results point towards the incorporation of primary lateral lymph node size into treatment planning.
Advances in Multiplexed Microfluidics for Infectious Disease Detection
Authors: Shi Yiwei , Zhao Jiayi , Cao Guangwen 
Published: June 2023

Abstract
Microfluidics enables miniaturization, functionality, high throughput and reproducibility of multi-pathogen detection. Multiplexed microfluidic devices are electrochemical sensor-based, optical sensor-based, immunosensor-based and paper-based multiplexed microfluidics. However, the simultaneous detection of multiple pathogens is limited due to the complexity and diversity of infectious disease sources and mutual interference among analytes. This review provides an overview of recent advances in developing multiplex diagnostic microfluidic devices for detecting infectious diseases and discusses practical issues and perspectives. This review also covers microfluidic nucleic acid amplification strategies to improve detection sensitivity. Finally, we discuss the limitations and challenges in the design of multiplexed microfluidics.
Obstetric, maternal, and neonatal outcomes after Pilates exercise during pregnancy: A systematic review and meta-analysis
Authors: Zaman Amal Yaseen 
Published: June 2023

Background:
One of the major exercises to improve the psychological and physical functions of pregnant women is Pilate’s exercises. The aim of this study is to collect evidence about Pilates exercise effects on many consequences in pregnant women, such as maternal and neonatal outcomes and obstetric outcomes.

Methods:
PubMed, Clinical Key, Cochrane Library, Scopus, Embase, and Web of Science were scoured from their inception. Research comparing Pilates during pregnancy to other methods, or to the control was included. For randomized controlled trials (RCTs), The researcher utilized the Cochrane risk of bias tool; for non-RCTs, risk of bias tool to assess non-randomized studies of interventions, was used for cohort studies, and the national heart, lung, and blood institute tool. Meta-analysis was done using the Review manager 5.4. For continuous data, calculated the mean difference and 95% confidence interval (CI), and the risk ratio (RR) and 95% CI for dichotomous data.

Results:
Finally, 13 studies included with a total number of 719 pregnant women. The analysis showed that the Pilates group was significantly more likely to deliver vaginally than the control group (RR = 1.21, 95% CI [1.05 to 1.41], P value = .009). Women in the Pilates group were significantly lower than the control group to have Cesarean delivery (RR = 0.67, 95% CI [0.48–0.94], P value = .02). Moreover, Pilates-treated women were less likely to gain weight during pregnancy than control (mean difference = −3.48, 95% CI [−6.17 to −0.79], P value = .01).

Conclusion:
Pilates exercise improved the outcomes of pregnant women. It decreases the rate of Cesarean delivery and the time of delivery. Moreover, Pilates has a role in decreasing weight gain in pregnant women. As a result, that may improve the pregnancy experience for women. However, more RCTs needed with larger sample sizes to assess the Pilates effect on neonatal outcomes.
Efficacy and safety of a combination of emotional freedom technique with acupuncture versus acupuncture alone to treat psychiatric symptoms in Parkinson’s disease: A protocol for a randomized, assessor-blind, parallel-group clinical trial
Authors: Kang Dong-Hoon , Kim Ju-Yeon , Park Yang-Chun , Yoo Ho-Ryong , Jung In Chul 
Published: June 2023

Background:
Parkinson’s disease (PD) is one of the most prevalent neurodegenerative diseases in world. As some psychiatric symptoms degrade the quality of life of patients with PD, a novel alternative non-pharmacological treatment is required. Acupuncture appears to be an effective and safe treatment for PD. The emotional freedom technique (EFT) is a type of psychological therapy that alleviates psychiatric symptoms by stimulating acupoints. In this study, we will compare the efficacy and safety of a combination of the EFT and acupuncture and acupuncture alone.

Methods:
This study is a randomized, assessor-blind, parallel-group clinical trial. Eighty participants will be equally divided into experimental and control groups. Each participant will receive a total of 24 interventions over 12 weeks. The experimental group will receive EFT combined with acupuncture and the control group will receive acupuncture alone. The primary outcome is the change in the Beck Depression Inventory score from baseline to 12 weeks, and the secondary outcomes include change in the following variables: Beck Depression Inventory, Parkinson’s disease sleep scale, State-Trait Anxiety Inventory, the Korean version of the Fatigue, Resistance, Ambulation, Illnesses, and Loss of weight questionnaire scale, and unified Parkinson’s disease rating scale III and exercises.

Discussion:
Acupuncture is a safe and effective treatment for motor and nonmotor symptoms in PD, and EFT appears to be safe and effective for a variety of psychiatric symptoms. In this study, we will investigate the potential of EFT combined with acupuncture to improve psychiatric symptoms in PD.
Craniotomy hematoma was removed from the bone flap and multiple holes were drilled on the bone flap: A case report
Authors: Zhu Wei , Ma Lei , Li Tuo , Chen Hongguang 
Published: June 2023

Background:
Chronic subdural hematoma (CSDH) is a common disease in neurosurgery department. Burr-hole drainage is the main surgical treatment. And the recurrence rate is as high as 25%.

Case Report:
In this case, a male patient with CSDH in the left frontotemporal parietal region underwent 2 drilling and drainage operations in the local hospital, but the hematoma recurred after operations. Being unable to bearing the repeated and progressive aggravation of headache, he came to our hospital for treatment. After considering the comprehensive situation, we use a new surgical method, removal of hematoma by drilling multiple holes in the lateral skull, to cure the patient.

Conclusions:
We get inspirations from the treatment of moyamoya disease surgery, through the bone holes the scalp forms many “meat column” like structures which have powerful capability in absorption, so the scalp could deep into the hematoma, then the CSDH could be cured. Provide a new surgical method for the treatment of refractory CSDH.
One-stage reconstruction using a fibula osteocutaneous free flap and an anterolateral thigh free flap for an extensive composite defect after en bloc resection of squamous cell carcinoma on the mouth floor, mandible, and anterior neck: A CARE-compliant case report
Authors: Shin SeHo , Kim KiHyun , Woo SangSeok , Kim KyungMin , Lee JunWon , Kim SeongHwan , Choi JaiKoo , Lee DongJin , Suh InSuck 
Published: June 2023

Rationale:
Although regional metastasis to the lymph nodes is common in advanced oral cancer, extensive local invasion into surrounding structures such as the mandible, skin and soft tissue of the neck, and masticator space is relatively rare. Sometimes surgical treatment cannot be performed and only palliative chemotherapy and radiation therapy are offered to preserve the quality of life of patients with advanced oral cancer. Nevertheless, the surgical resection of tumors remains the most effective treatment. This study presents a case of aggressive mouth floor cancer in which extensive composite defects on the mouth floor, oral mucosa, mandible, skin and soft tissue of the neck caused by tumor resection were reconstructed.

Patient concerns:
A 66-year-old man and a 65-year-old man with no significant personal or family history visited our clinic due to a large and multiple masses on the floor of the mouth and both sides of the neck.

Diagnosis:
Histopathological evaluation of the biopsy specimen revealed squamous cell carcinoma.

Interventions:
A fibula osteocutaneous free flap and customized titanium plate were used for the intraoral lining. Mandibular reconstruction was performed using a 3D-printed bone model, and an anterolateral thigh free flap was used to resurface the anterior of the neck.

Outcomes:
Reconstruction using this method was successful, and excellent functional and aesthetic outcomes were achieved without cancer recurrence.

Lessons:
This study show that the reconstruction of extensive composite defects of the oral mucosa, mandible, and neck soft tissue following surgical resection of mouth floor cancer can be performed in a single-stage operation. Through a single-stage reconstruction, both excellent functional aspects without cancer recurrence and satisfactory aesthetic outcomes can be obtained.
The efficacy of vericiguat for heart failure: A meta-analysis of randomized controlled trials
Authors: Ma Guofang , Pan Yuefang , Qu Chaoyi , Li Feng 
Published: June 2023

Background:
The efficacy of vericiguat was elusive for heart failure. This meta-analysis aimed to explore the efficacy of vericiguat for heart failure.

Methods:
PubMed, EMbase, Web of Science, EBSCO, and Cochrane library databases have been searched through October 2022 and we included randomized controlled trials reporting the effect of vericiguat versus placebo in patients with heart failure.

Results:
Four randomized controlled trials were included in the meta-analysis. Compared with placebo group for heart failure, vericiguat treatment was able to substantially improve the composite outcome of cardiovascular death or heart failure hospitalization (odds ratio [OR] = 0.87; 95% confidence interval [CI] = 0.78 to 0.97; P = .02), but unraveled no obvious impact on hospitalization for heart failure (OR = 0.89; 95% CI = 0.79 to 1.00; P = .05), death from cardiovascular causes (OR = 0.93; 95% CI = 0.77 to 1.13; P = .48), death from any cause (OR = 0.96; 95% CI = 0.84 to 1.10; P = .56), adverse events (OR = 0.95; 95% CI = 0.84 to 1.08; P = .42) or serious adverse events (OR = 0.92; 95% CI = 0.82 to 1.02; P = .12).

Conclusions:
Vericiguat treatment may benefit to treat heart failure.
Aggressive systemic mastocytosis of colon and lymph node: A case report
Authors: Xun-Ze Shen , Liu Fang , Lin Chen , Sun Yi-Feng 
Published: June 2023

Rationale:
Mastocytosis is a group of rare neoplastic diseases characterized by monoclonal proliferation of mast cells in the skin or other tissues and organs, including cutaneous mastocytosis and systemic mastocytosis (SM). Mastocytosis can also occur in the gastrointestinal tract, mostly manifested as increased mast cells dispersed in various layers of the intestinal wall; a few may present as polypoid nodules, but rarely as soft tissue mass formation. Pulmonary fungal infections mostly occur in patients with low immune function and have not been reported in the literature as the initial manifestation in patients with mastocytosis. In this case report, we present the enhanced computed tomography (CT), fluorodeoxyglucose (FDG) positron emission tomography/CT, and colonoscopy findings of a pathologically confirmed patient with aggressive SM of the colon and lymph nodes and extensive fungal infection of both lungs.

Patient concerns:
A 55-year-old female patient visited our hospital because of repeated cough for more than half a month. Laboratory tests revealed a significantly high CA125 serum level. Chest CT showed multiple plaques and patchy high-density shadows in both lungs, and a small amount of ascites was observed in the lower-level image. Abdominal CT revealed a soft tissue mass with an ill-defined boundary in the lower ascending colon. Whole-body positron emission tomography/CT images showed multiple nodular and patchy density-increasing lesions with significantly increased FDG uptake in both lungs. The wall of the ascending colon in the lower segment was significantly thickened with soft tissue mass formation, and retroperitoneal lymph node enlargement was accompanied by increased uptake of FDG. Colonoscopy revealed a soft tissue mass at the base of the cecum.

Diagnosis:
Colonoscopic biopsy was performed and the specimen was diagnosed with mastocytosis. At the same time, a puncture biopsy was also performed on the patient’s lung lesions, and pulmonary cryptococcosis was considered a pathological diagnosis.

Interventions:
The patient was in remission after repeated treatment with imatinib and prednisone for 8 months.

Outcomes:
In the ninth month, the patient suddenly died of a cerebral hemorrhage.

Lessons:
Gastrointestinal involvement due to aggressive SM presents with nonspecific symptoms and different endoscopic and radiologic findings. This is the first report of a single patient with colon SM, retroperitoneal lymph node SM, and extensive fungal infection in both lungs.
Secondary hemophagocytic lymphohistiocytosis associated with heat stroke: A case report and review of literature
Authors: Wi Wongook , Yoon Kyoung Won , Kim Hyo Jin 
Published: June 2023

Rationale:
Hemophagocytic lymphohistiocytosis (HLH) is a syndrome with potentially fatal consequences that results from an excessive immune response caused by malfunctioning natural killer cells and cytotoxic T lymphocytes. Secondary HLH, which is the predominant type in adults, is associated with various medical conditions, including infections, malignancies, and autoimmune diseases. Secondary HLH associated with heat stroke has not been reported.

Patient concerns:
A 74-year-old male was admitted to the emergency department after being unconscious in a 42°C hot public bath. The patient was witnessed to be in the water for more than 4 hours. The patient’s condition was complicated by rhabdomyolysis and septic shock, which were managed with mechanical ventilation, vasoactive agents, and continuous renal replacement therapy. The patient also showed evidence of diffuse cerebral dysfunction.

Diagnoses:
While the patient’s condition initially improved, the patient developed a fever, anemia, thrombocytopenia, and an acute rise in total bilirubin, which, we suspected, was caused by HLH. Further investigations revealed elevated serum ferritin and soluble interleukin-2 receptor levels.

Interventions:
The patient received 2 cycles of serial therapeutic plasma exchange to lower the endotoxin burden. To manage HLH, high-dose glucocorticoid therapy was done.

Outcomes:
Despite the best efforts, the patient did not recover and expired from progressive hepatic failure.

Lessons:
We report a novel case of secondary HLH associated with heat stroke. Diagnosing secondary HLH can be difficult since clinical manifestations of the underlying disease and HLH may present simultaneously. Early diagnosis and prompt initiation of treatment is required to improve the prognosis of the disease.