bluuebunny/crossref_metadata_2025_split huggingface dataset:
Peculiarities of differential diagnosis of malignant and benign ovarian tumors in girls (case report of immature teratoma of the ovary)
Authors: Sibirskaya Elena V. , Karachentsova Irina V. , Skapenkov Ivan N. , Naumenko Andrey A. , Petinov Ivan I. , Nikiforova Polina O. 
Published: July 2023

Germ cell tumors are a heterogeneous group of tumors differentiating from primary germ cells of embryonic gonads and their derivatives. This case report demonstrates the diagnostic algorithm and diagnostic tactics of a rarely encountered histological type of ovarian germ cell tumor.
Perspective of Farm Gate Agroforestry Product in the Emergency of the Chepangs Community: A Case Study of Mega earthquake of Central Nepal
Authors: Chetry Mr. Raju , Kant Silwal Dr. Uma , Kanel Mr. Damodar 
Published: July 2023

Agroforestry is one of the major sources of sustenance among the people of Nepal, and only one mode of livelihood at the Chepangs in present days. National Seismological Centre (NSC-Nepal), recorded mega earthquake (7.8 Mw) that hit the country on the 25th of April in 2015, and many of its aftershocks had catastrophic effects. As a result, big structures collapsed, which incurred great losses on fabrication, collection centers, schools, markets, and other potential government, and private service centers. Continue aftershocks, and after two weeks later another earthquake hit (6.3 Mw) jeopardize Chepangs food security in Makwanpur.
This study was designed to find out the food security, and market situation of agroforestry product of the Chepang community in Raksirang (One of the Severely affected VDC in Makwanpur district) due to the earthquake. A total of 40 households (12 %) were selected as a sample size. Questionnaires, Focus Groups Discussion (FGD), Observation, key informant interview tool were applied, and detail information from District Disaster Response Committee (DDRC), and emergency support agencies (UNICEF, WFP, PLAN, TEAR Fund) were consulted for additional narrative information.
The research illustrated that approx. 370 hectares of land, with agroforestry plantation, ruptured by the earthquake, caused severe damages to the household’s structure, and a loss of food-stock up to 2/3 portion. Instead of regular cereal (rice, maize, and vegetables) intake, banana (Musa paradisiaca) consumption was in high scale during the emergency period. The market price of rice increased by 18.9 % due to demand. Income variation found before, and after the disaster. Prior to earthquake, agroforestry-livestock product was accelerating, but after the earthquake, agroforestry remains the only one means of livelihood among Chepangs. Nearly, half of agroforestry production of banana (Musa paradisiaca), citrus (Citrus lemon), pineapple (Ananus comosus) and broom grass (Thysanolaena maxima) declined, which was ready to harvest within 2-3 weeks. Heavy demands of agroforestry product above lay high farm gate prices (Up to doubled) helps surveyed community to continue livelihood from its sale. Nearly, 75 % respondents migrated to safe location due to land rupture, ongoing aftershocks, and occurring landslides. Overall food security situation in this community is acute, and challenging for the days to come. Immediate, and long-term response through relief agency concerning food, shelter, and safe stocking of agroforestry product is required. Agroforestry plantation and technical support to increase production, and to balance the food security and livelihood for longer period are the upcoming challenges in the coming days.
ARG1 is a potential prognostic marker in metastatic and recurrent endometrial cancer
Authors: Tran Dinh Nam , Rozen Valery , Hunter Mark I. , Kim Tae Hoon , Jeong Jae-Wook 
Published: July 2023

Abstract
Endometrial cancer (EC) is the most common gynecologic malignancy. While the majority of patients present with early-stage and low-grade EC and have an excellent prognosis, a subset has metastatic disease at presentation, or develops distant recurrence after initial treatment of the primary. However, the lack of prognostic biomarkers for metastatic EC is a critical barrier. Arginase 1 (ARG1) regulates the last step of the urea cycle, and an increase in ARG1 has been correlated as a poor prognostic factor in a variety of cancers. In the present study, ARG1 expression was evaluated as a potential prognostic marker for metastatic EC in endometrial hyperplasia and cancer of mice with Pten mutation as well as Pten and Mig-6 double mutations. While Pten mutation in the uterus is not sufficient for distant metastasis, mice with concurrent ablation of Mig-6 and Pten develop distant metastasis. Our immunostaining and RT-qPCR analysis revealed that the expression of ARG1 in early stage of EC as well as endometrial hyperplasia from mice deficient in Mig-6 and Pten mutations significantly increased compared to Pten mutation in the uterus. The results suggest that a high level of ARG1 is associated with poor prognosis in association with EC of mouse.
Psk-1 virulence gene-induced pulmonary and systemic tuberculosis in a young female with normal immune function
Authors: wu fan , Yang Bin , Xiao Yan , Chen Hongyi , Hu Xinlan , Pan Yanyu , Ren Lili , Chen Yusheng , Li Hongru 
Published: July 2023

Abstract
Background
Tuberculosis is a chronic infectious disease and an important public health threat. Despite China’s achievements in controlling tuberculosis, the prevalence of this disease is still very high, with 895,000 new cases annually. This case report aimed to ascertain why a Mycobacterium tuberculosis strain caused such a severe infection in a young adult with normal immune function.
Case presentation:
We present the case of a young female with normal immune function without the history of receiving bacillus Calmette–Guérin vaccine, who suffered from severe pulmonary tuberculosis and secondary systemic disseminated tuberculosis. The M. tuberculosis was isolated from the bronchoalveolar lavage fluid of the patient. Further, we analysed the whole-genome sequence of the strain and designated it BLM-A21. Additional M. tuberculosis genomes were selected from the Virulence Factor Database (http://www.mgc.ac.cn/cgi-bin/VFs/genus.cgi?Genus=Mycobacterium), developed by the bioinformatics research team of the Institute of Pathogenic Biology, Chinese Academy of Medical Science. The evolutionary tree of disseminated tuberculosis was then built using the PhyML maximum likelihood software. Further gene analysis revealed that BLM-A21 has similar virulence genes to the strains CDC 1551 and H37Rv, which have lower dissemination, except for the pks-1 gene, which may be the key virulence gene responsible for the high dissemination ability of this M. tuberculosis strain.
Conclusions
We speculated that the pks-1 virulence gene of this strain induced severe pulmonary tuberculosis and secondary systemic disseminated tuberculosis in this adult female with normal immune function.
Mechanisms of rainfall-induced landslides and interception dynamic response: A Case Study of the Ni changgou Landslide in Shimian, China
Authors: Li Yu , Yang Xuezhi , Hu Xiao , Wan Liyan , Ma Erlong 
Published: July 2023

Abstract
Geological hazards, especially landslides and mudslides, are frequent in Caoke County, Sichuan Province, China. In September 2022, a large-scale mudslide occurred in Ni changgou with continuous rainfall, and it caused great damage to the surrounding roads and downstream village buildings. The mechanical parameters of the soil were obtained through a basic investigation of the landslide characteristics of Nichanggou. Upon that, the finite element-discrete element method was used to reconstruct the three-dimensional numerical model of the landslide on the right bank of Nichanggou, and the initiation mechanism of rainfall on landslide and the formation of debris flow impact dam process were simulated. Furthermore, the pore pressure, stability coefficient as well as displacement of the landslide body were analyzed, it turned out that under long-term continuous rainfall conditions, the slope safety factor decreases with the increase of rainfall time by analyzing the pore pressure, stability coefficient and displacement of the landslide body, which exist under different conditions of heavy and extreme rainstorms. Then the laws affecting landslide initiation and damage characteristics could be found out. The duration of landslide movement is about 200 s, the maximum average velocity of the landslide reaches 4.85 m/s, and the average movement distance is close to 500 m. These measured data matches well with that simulated landslide mound morphology.In addition, as the downstream debris flow washout is part of the initiating material source, the topography and debris flow are simplified accordingly, the whole process of impact, climbing height and back silting of the debris flow impact blocking structure is reproduced. The coupled method shows the whole process of debris flow from formation, to blocking, The results show desired effect on the mudflow prevention and management.
The design and implementation of intelligent ubiquitous learning Multi-Agent Context-Aware System
Authors: Guettala Manel , Bourekkache Samir , Kazar Okba , Harous Saad , Zouai Meftah 
Published: July 2023

Abstract

Advances in technology in education have had a profound impact on Human Computer Interaction. The ubiquity of computer technology means that ubiquitous learning (U-learning) has really arrived on the scene. In this article, we explore how emerging technologies can make learning more affordable and accessible. Our aim is to present a new approach to ubiquitous learning, which uses a multi-agent system in order to facilitate the learning process. To develop the multi-agent system, we used JADE-LEAP (Lightweight and Extensible Agent Platform). The application developed adapts several parameters according to the dynamic situation of the learner's contextual information. We used contextual technologies such as GPS sensors and 4G networks to obtain the dynamic situation. This application provides a set of functionalities that can be used by learners via devices anywhere and anytime, and supports formal learning. Finally, to verify the usefulness of this application, we conducted a case study with different scenarios. Computer science students from various Algerian universities took part in the study. .
Transfer of Chromium from Environment to Fish in East Kolkata Wetlands – Evaluation by Structural Equation Modeling
Authors: Ghosh Debkanta , Saha Samir Kumar , Kaviraj Anilava , Saha Subrata 
Published: July 2023

Abstract

Chromium (Cr) is a significant pollutant in the effluents from leather industries as well as in domestic city sewage. Cr was determined in water, sediment and different tissues (gill, muscle, intestine, liver, and kidney) of Nile tilapia, Oreochromis niloticus harvested from wastewater-fed aquaculture (WFA) ponds situated at Bamonghata, Bantala, Chowbaga and Chingrighata of East Kolkata Wetlands (EKW), a Ramsar site in West Bengal, India. The results showed that Cr concentration in surface water ranged between 0.05 to 0.15 mg/L, while Cr was detected at high concentration (100 – 300 mg/kg) in the sediment soil of the first three WFAs and in moderate concentration (50 – 110 mg/kg) in Chingrighata WFA. Average Cr concentrations in the tissues were ranked in the following sequence: kidney>liver>intestine>gill>muscle. However, the extent of accumulation of Cr in different tissues varied between the WFAs. We used Structural Equation Modelling (SEM) to determine the route of Cr transfer. The fitness of the model was evaluated by the performance measures. Cr accumulation pathways varied between the sites depending upon the level of Cr in water or sediment. Except for Bamonghata WFA, sediment was found as the principal source of accumulation of Cr in different tissues of O. niloticus. Cr refluxed from sediment into overlying water and accumulated in fish either through the food chain or through direct accumulation from water. In Bamonghata WFA, the role of sediment in the transfer of Cr could not be established due to the high depth of water or biological non-availability of Cr in the sediment. It is concluded from this study that fish reared in the WFAs of EKW are still not hazardous in respect of Cr, but require proper management to avoid the influx of Cr-containing effluents into the WFAs.
In vitro antibiofilm activity of tormentic acid against Pseudomonas aeruginosa
Authors: Dakwa Ruvarashe , Mozirandi Winnie , Mukanganyama Stanley 
Published: July 2023

Abstract

Background: Biofilm formation is the cause for the prevalence of nosocomial infections. Pseudomonas aeruginosa is one of the leading cause of nosocomial infections and affects more than 2 million patients. Infections caused by P. aeruginosa can be very serious and life-threatening. P. aeruginosa can adhere to biotic and abiotic surfaces through the organisation of bacterial biofilms known to confer drug resistance. Consequently, the logical and strategic intervention against bacterial biofilms is the use of multi-targeted combinational therapies. The present study reports the antibacterial and antibiofilm effect of tormentic acid and Callistemon citrinus extracts on P. aeruginosa.
Methods: Tormentic acid, hydroethanolic extract, and DCM: methanol extract from C. citrinus were studied for their inhibitory effects on the growth of P. aeruginosa using the microbroth dilution method. Their effect on biofilms was studied using a crystal violet.
Results: These compounds did not effectively inhibit growth because the minimum inhibitory concentration (MIC) was greater than 100 µg/ml. The standard drug, ciprofloxacin inhibited growth with an MIC of 0.25 µg/ml. Ciprofloxacin had a minimum biofilm inhibitory concentration (MBIC) greater than 1 µg/ml. Tormentic acid and the two extracts were able to significantly inhibit the adhesion of P. aeruginosa cells, which led to an indirect reduction of biofilms formed at 100 µg/ml. The result of the biofilm detachment assay showed that tormentic acid and DCM: methanol extract had a significant effect on biofilm disruption while the hydroethanolic extract had no effect on biofilm detachment. Capsular polysaccharide analysis showed that the test samples tormentic acid, hydroethanolic, and DCM: methanol extracts significantly reduced the amount of capsular polysaccharide content at 100 µg/ml. Ciprofloxacin at 0.25 µg/ml (MIC) did not reduce the amount of extracellular polysaccharide formed.
Conclusions: Tormentic acid and extracts significantly reduced amount of extracellular polysaccharide content, which are an important requirement in cell adhesion process resulting in reduced amount of biofilms formed. Tormentic acid can be considered as a candidate compound for the development of antibiofilm drugs.
Integrated Effect of Bacillus pumilus and Mineral Fertilizers on Growth Parameters on Potato (Solanum tuberosum L. cv. Citlali)
Authors: Paz-Osorio Denisse Monserrat de la , Paz Laura Sanchez , Portilla-Lopez Nadia de la , Vaca-Paulín Rocío , Fuente Jorge Lugo-de la , Wong-Villarreal Arnoldo , Águila-Juárez Pedro del , Mora-Herrera Martha Elena , Ocampo Gustavo Yañez 
Published: July 2023

Abstract

Background and aims Plant Growth Promoting Rhizobacteria can make available phosphorus (P) for crops of economic and food importance such as Solanum tuberosum L. (potato), allowing the application of fertilizers to be reduced. The aim was evaluate the integral effect of Bacillus pumilus (phosphate solubilizer) alone and with 30 kg P/ha, as well as a conventional fertilization of 60 kg P/ha on growth, production and sprouting of potato under greenhouse conditions. Methods Five treatments were assayed: NPK 100% (T1), NK 100% (T2), B. pumilus, BP (T3), BP + NPK 50% (T4), BP + NK 50% (T5). To measure the efect of mixture bacteria and fertilizer on crop development, we recorded plant height, fresh and dry weights, number of internodes, SPAD values, fresh weight of tubers, total number of tubers, number of tubers with diameters greater than or equal to 15 mm, length and width of sprouts. Results Potato growth parameters indicated a similarity in: plant height (21.65 cm, 20.95 cm), number of internodes (12.42, 13.11), fresh (9.97 g, 5.53 g) and dry weights (1.87 g, 1.08 g) of the treatments T1 and T4, respectively. In mini-tuber production and sprouting, tuber fresh weight (g), number of total tubers, tuber diameter ≥ 15 mm, and sprout width were statistically significant at T4 respect to treatment with B. pumilus alone. Conclusion Compared to conventional mineral fertilization B. pumilus with 50% mineral fertilizer, have equal effect on plant growth values of potato, production and sprouting of mini tubers.
Flaxseed Derived Secoisolariciresinol diglucoside Enriched Fermented Milk Exhibits Antiosteopenic Effects in Ovariectomized Rats
Authors: Thomas Elizabeth , Panjagari Narender Raju , Ganguly Sangita , Deepika Sameni , Kapila Suman , Singh Ashish Kumar 
Published: July 2023

Abstract
The current research aims to validate the effect of flaxseed lignan (secoisolariciresinol diglucoside (SDG)) enriched dahi (analogues to yoghurt) in preventing menopausal osteoporosis in-vivo using an ovariectomized (OVX) rat model. Forty female Albino Wistar rats (3 months old, > 180 g) were divided into sham, OVX control, OVX + control dahi, OVX + SDG enriched dahi groups (n = 8). The results of the animal study indicated the serum calcium and estrogen levels and the bone ash calcium were decreased by 32.27%, 30.95% and 48.46% respectively after ovariectomy, compared to the sham group. The administration of the control dahi increased the serum calcium and estrogen levels as well as the bone ash calcium by 6.26%, 38.62% and 44.57%, while, the daily administration of the SDG enriched dahi (20 g, ~ 260 mg SDG) for eight weeks increased them by 37.37%, 85.52% and 82.34%, respectively, compared to OVX group. The micro-CT analysis of proximal tibial metaphysis region revealed that the ovariectomy decreased the bone mineral density (BMD) and trabecular thickness (Tb.Th) by 11.06% and 90.69%, while the trabecular separation (Tb.Sp.) was increased by 90.69% compared to the sham control group rats. The administration of the control dahi increased the BMD and Tb.Th by 8.85% and 10.04%, and decreased Tb.Sp by 22.13%, whereas the supplementation of SDG enriched dahi increased them by 13.84% and 26.10%, respectively and decreased the Tb.Sp by 88.82% compared to OVX rats. Similar results were obtained in the micro-CT analysis of distal femur epiphysis region. Hence, based on the above results, it could be concluded that the developed product could be useful in alleviating postmenopausal osteoporosis.
The emergence of clonally diverse carbapenem-resistant Enterobacter cloacae complex in West Bengal, India: A dockyard of β-lactamases periling nosocomial infections
Authors: Das Surojit , Mallick Abhi , Barik Mili , Sarkar Soma , Saha Puranjay 
Published: July 2023

Abstract

Carbapenem-resistant Enterobacter cloacae complex (CRECC) constitutes a global public health threat challenging clinical treatment and infection control, especially in low- and middle-income countries such as India. We analyzed the antimicrobial susceptibility, major β-lactamase genes, plasmid profiles, and genetic relatedness to understand the molecular epidemiology of CRECC clinical isolates (n=44) in West Bengal, India, during 2021-2022. The majority (>55%) of the isolates were resistant to fluoroquinolones, aminoglycosides, and co-trimoxazole, even >20% for tigecycline and >35% were extensively drug-resistant. Co-β-lactamase production was categorized into twenty-seven types, importantly NDM (84%), OXA-48 (40%), TEM (61%), CTX-M (46%), OXA-1 (55%), and MIR (27%). The NDM-1 and OXA-181 were major variants with the observations of two novel NDM-24 and -29 variants. Wide-range of plasmids (2 to >212kb) were harbored by the β-lactamase-producing isolates: small (91%), medium (27%), large (9%), and mega (71%). IncX3, ColE1, and HI2 were noted in about 30% of isolates, while IncF and R were carried by <20% of isolates. The clonally diverse CRECC isolates were noted to cause cross-infections, especially at superficial site, bloodstream, and urinary-tract. This is the first molecular surveillance on CRECC in India. The study isolates serve as the dockyard of NDM, TEM, and CTX-M harboring a wide range of plasmids. The outcomes of the study may strengthen local and national policies for infection prevention and control practices, clarifying the genetic diversity among CRECC. Extensive genomic study may further intersect the relationships between these different plasmids, especially with their sizes, types, and antibiotic resistance markers.
Cyr61 promotes inflammation in EtOH-induced RAW264.7 cell by regulating Wnt/β-catenin signaling pathway
Authors: Shi Wanrong , Chen Bangjie , Yang Wenzhi , Yang Fan , Wang Xinyi , Li Jiajin , Yang Yipin , Sun Yuansong , Li He 
Published: July 2023

Abstract
Growth factors induce Cysteine-rich 61 (Cyr61), a member of the Cyr61/CTGF/Nov (CCN) family of proteins, which interacts with a number of integrins and with the proteoglycans heparan sulfate. Cyr61 participates in angiogenesis, cell differentiation, death, and the production of the extracellular matrix. Recent data have shown that Cyr61 is a key chemokine in controlling inflammation and fibrosis in liver injury, but until recently, uncertainty surrounds Cyr61's part in alcoholic liver disease (ALD). This work investigated Cyr61 expression in RAW264.7 cells induced by EtOH and its impact on pertinent cell function. The findings showed that Cyr61 was increased in EtOH-induced RAW264.7 cells and that it may have an adverse effect on cell survival, stimulate the release of inflammatory cytokines, boost cell growth, and block apoptosis. The signaling pathway for Wnt and β-catenin may be activated as part of the mechanism. The involvement of Cyr61 in EtOH-induced RAW264.7 cells was disclosed by our investigation, offering a fresh target for the prevention and treatment of ALD.