bluuebunny/crossref_metadata_2025_split huggingface dataset:
Understanding aggression in autism across childhood: Comparisons with a non‐autistic sample
Authors: Quetsch Lauren B. , Brown Cynthia , Onovbiona Harlee , Bradley Rebecca , Aloia Lindsey , Kanne Stephen 
Published: April 2023

AbstractAs many as half of all autistic youth face challenges with aggression. And while research in this area is growing, the prevalence and characterization of aggressive behaviors across autistic development remains poorly understood. This lack of knowledge on the autistic experience is further clouded as aggression is rarely compared against non‐autistic youth samples. To address this gap in the literature, the present study compared autistic children (N = 450) to non‐autistic children (N = 432) on multiple caregiver‐report measures of aggressive behavior and associated constructs (i.e., anger, disruptive behavior) across key developmental periods (<6, 6–12, 13–17 years) via a cross‐sectional design. Outcomes indicated higher levels of verbal aggression and behavioral intensity for autistic youth across development. Further, autistic children under age 6 had more significant levels of physical aggression than non‐autistic peers; however, these levels became equal to non‐autistic peers as the youths aged. Implications for differences in the presence of aggressive behavior as well as possible treatment options for aggression are discussed.
High baseline tumor burden‐associated macrophages promote an immunosuppressive microenvironment and reduce the efficacy of immune checkpoint inhibitors through the IGFBP2‐STAT3‐PD‐L1 pathway
Authors: Wen Zhaowei , Sun Huiying , Zhang Zhihua , Zheng Yannan , Zheng Siting , Bin Jianping , Liao Yulin , Shi Min , Zhou Rui , Liao Wangjun 
Published: April 2023

AbstractBackgroundSeveral clinical studies have uncovered a negative correlation between baseline tumor burden and the efficacy of immune checkpoint inhibitor (ICI) treatment. This study aimed to uncover the specific mechanisms underlying the difference in sensitivity to ICI treatment between tumors with high (HTB) and low (LTB) tumor burden.MethodsFor in vivo studies, several mouse models of subcutaneous tumors were established, and transcriptome sequencing, immunohistochemistry, and flow cytometry assays were used to detect the immune status in these subcutaneous tumors. For in vitro experiments, co‐culture models, cytokine antibody arrays, western blotting, flow cytometry, and enzyme‐linked immunosorbent assays were used to explore the underlying molecular mechanismsResultsWe found that MC38 or B16 subcutaneous tumors from the HTB group did not show any response to anti‐programmed cell death protein‐1 (PD‐1) therapy. Through flow cytometry assays, we found that the infiltration with CD8+ T cells was significantly decreased whereas M2‐like macrophages were enriched in subcutaneous tumors of HTB groups compared with those of LTB group. These changes were not affected by the initial number of injected tumor cells or tumor age, nor could they be reversed by surgical tumor reduction. Intraperitoneal colony‐stimulating factor 1 receptor (CSF‐1R) inhibitor PLX3397 injection at different time points of tumor growth only had an effect when administered in the early tumor stage to maintain the “heat” of the tumor microenvironment during the process of tumor growth, thereby achieving a response to ICI treatment when the tumor grew to a large size. Mechanistically, we found that insulin‐like growth factor binding protein 2 (IGFBP2) expression levels were significantly elevated in HTB tumor tissues. IGFBP2 promoted the programmed death‐ligand 1 (PD‐L1) expression in M2‐like macrophages by activating signal transducer and activator of transcription 3 (STAT3), and PD‐L1+ M2‐like macrophages exerted an immunosuppressive effect by inhibiting the proliferation and activation of CD8+ T cells in a PD‐L1‐dependent fashion.ConclusionsThis study suggested that the low efficacy of ICI treatment in HTB tumors is mainly attributed to the intratumoral accumulation of PD‐L1+ M2‐like macrophages via the IGFBP2‐STAT3‐PD‐L1 signaling pathway and their substantial inhibitory effects on T cell proliferation and activation.
A modeling study to estimate prostate cancer‐specific mortality on active surveillance for men with favorable intermediate‐risk prostate cancer: Results from the SEARCH cohort
Authors: Kuhlmann Paige K. , Oyekunle Taofik , Klaassen Zachary , Amling Christopher L. , Aronson William J. , Cooperberg Matthew R. , Kane Christopher J. , Terris Martha K. , Freedland Stephen J. 
Published: April 2023

AbstractPurposeLimited data exist to help surgeons decide between active surveillance (AS) versus treatment for men with favorable intermediate risk (FIR) prostate cancer. To estimate the theoretical excess risk of prostate cancer‐specific mortality (PCSM) with AS versus radical prostatectomy (RP), we determined the risk of PCSM in FIR men undergoing RP and modeled the PCSM risk for AS using a range of increased PSCM scenarios ranging from 1.25x to 2x higher relative to RP.Materials and MethodsWe retrospectively reviewed data from men undergoing RP from 1988 to 2017 at 8 Veterans Affairs hospitals within the SEARCH cohort. Men with FIR PC were identified using the NCCN risk criteria. Risk of PCSM at 5, 10, and 15 years after RP was estimated. Using these estimates, PCSM was then modeled for AS using a range of increased risk of PCSM relative to RP ranging from 1.25x to 2x higher.ResultsFor the 920 FIR men identified, 5‐, 10‐, and 15‐year survival estimates for PCSM after RP were 99.9%, 99.0%, and 97.8%, respectively. If the risk of PCSM on AS were 1.25–2x greater than RP, there would be 0.54%–2.17% excess risk of PCSM at 15 years.ConclusionsThe risk of death for FIR after RP is very low. Assuming even modestly increased PCSM with AS versus RP, the excess risk of death for AS in FIR is low even up to 15 years. These data support the consideration of AS as a relatively safe alternative to RP in FIR men, though prospective randomized trials are needed to validate these findings.
Therapeutic effects of anti‐GM2 CAR‐T cells expressing IL‐7 and CCL19 for GM2‐positive solid cancer in xenograft model
Authors: Sasaki Takahiro , Sakoda Yukimi , Adachi Keishi , Tokunaga Yoshihiro , Tamada Koji 
Published: April 2023

AbstractBackgroundWhile chimeric antigen receptor (CAR)‐T cell therapy has demonstrated excellent efficacy in hematopoietic malignancies, its clinical application in solid cancers has yet to be achieved. One of the reasons for such hurdle is a lack of suitable CAR targets in solid cancers.MethodsGM2 is one of the gangliosides, a group of glycosphingolipids with sialic acid in the glycan, and overexpressed in various types of solid cancers. In this study, by using interleukin (IL)‐7 and chemokine (C‐C motif) ligand 19 (CCL19)‐producing human CAR‐T system which we previously developed, a possibility of GM2 as a solid tumor target for CAR‐T cell therapy was explored in a mouse model with human small‐cell lung cancer.ResultsTreatment with anti‐GM2 IL‐7/CCL19‐producing CAR‐T cells induced complete tumor regression along with an abundant T cell infiltration into the solid tumor tissue and long‐term memory responses, without any detectable adverse events. In addition, as measures to control cytokine‐release syndrome and neurotoxicity which could occur in association with clinical use of CAR‐T cells, we incorporated Herpes simplex virus‐thymidine kinase (HSV‐TK), a suicide system to trigger apoptosis by administration of ganciclovir (GCV). HSV‐TK‐expressing anti‐GM2 IL‐7/CCL19‐producing human CAR‐T cells were efficiently eliminated by GCV administration in vivo.ConclusionsOur study revealed the promising therapeutic efficacy of anti‐GM2 IL‐7/CCL19‐producing human CAR‐T cells with an enhanced safety for clinical application in the treatment of patients with GM2‐positive solid cancers.
Accelerated epigenetic aging and myopenia in young adult cancer survivors
Authors: Gehle Stephanie C. , Kleissler Daniel , Heiling Hillary , Deal Allison , Xu Zongli , Ayer Miller Vanessa L. , Taylor Jack A. , Smitherman Andrew B. 
Published: April 2023

AbstractBackgroundYoung adult cancer survivors experience early aging‐related morbidities and mortality. Biological aging biomarkers may identify at‐risk survivors and increase our understanding of mechanisms underlying this accelerated aging.MethodsUsing an observational study design, we cross‐sectionally measured DNA methylation‐based epigenetic age in young adult cancer survivors at a tertiary, academic state cancer hospital. Participants were a convenience sample of consecutively enrolled survivors of childhood, adolescent, and young adult cancers treated with either an anthracycline or alkylating agent, and who were at least 3 months post‐treatment. Similarly aged healthy comparators were consecutively enrolled. Cancer treatment and treatment intensity were compared to DNA methylation‐based epigenetic age and pace of aging.ResultsSixty survivors (58 completing assessments, mean age 20.5 years, range 18–29) and 27 comparators (mean age 20 years, range 17–29) underwent DNA methylation measurement. Survivors were predominantly female (62%) and white (60%) and averaged nearly 6 years post‐treatment (range 0.2–25 years). Both epigenetic age (AgeAccelGrim: 1.5 vs. −2.4, p < 0.0001; AgeAccelPheno 2.3 vs. −3.8, p = 0.0013) and pace of aging (DunedinPACE 0.99 vs. 0.83, p < 0.0001) were greater in survivors versus comparators. In case–case adjusted analysis, compared to survivors with normal muscle mass, myopenic survivors had higher AgeAccelGrim (2.2 years, 95% CI 0.02–4.33, p = 0.02), AgeAccelPheno (6.2 years, 2.36–10.09, p < 0.001), and DunedinPACE (0.11, 0.05–0.17, p < 0.001).ConclusionsEpigenetic age is older and pace of aging is faster in young adult cancer survivors compared to noncancer peers, which is evident in the early post‐therapy period. Survivors with physiological impairment demonstrate greater epigenetic age advancement. Measures of epigenetic age may identify young adult survivors at higher risk for poor functional and health outcomes.
Anti‐CLL1‐based CAR T‐cells with 4‐1‐BB or CD28/CD27 stimulatory domains in treating childhood refractory/relapsed acute myeloid leukemia
Authors: Pei Kunlin , Xu Haoyu , Wang Pengfei , Gan Wening , Hu Zhengbin , Su Xiaoling , Zhang Hui , He Yingyi 
Published: April 2023

AbstractBackgroundThough the efficacy of anti C‐type lectin‐like molecule‐1 (CLL1) CAR T‐cells in refractory/relapsed acute myeloid leukemia (R/R‐AML) have been occasionally reported, the influence of co‐stimulatory domain CAR T‐cells is not investigated so far.MethodSeven R/R‐AML children treated with anti‐CLL1 CAR T‐cells were enrolled onto this preliminary comparison study. Among these seven patients, four received CD28/CD27‐based CAR T‐cells therapy, and three received 4‐1BB‐based CAR T‐cells therapy.ResultThe overall response rates were 75% and 66.7% in CD28/CD27 and 4‐1BB group respectively. All patients experienced grade 1 to 2 cytokine release syndrome, with only one patient experiencing grade 2 immune effector cell‐associated neurotoxicity syndrome. The maximum CAR T‐cells durations were 156 and 274 days for CD28/CD27 group and 4‐1BB group respectively. The 1‐yr overall survival rate was 57.1%.ConclusionsA preliminary similar efficacy/safety index was observed in anti‐CLL1‐based CAR T‐cells with 4‐1BB or CD28/CD27 co‐stimulatory elements in treating pediatric R/R‐AML.
Expression and interpretation of emotions in multilingual psychotherapy: A literature review
Authors: Verkerk Leila , Fuller Janet M. , Huiskes Mike , Schüppert Anja 
Published: April 2023

AbstractThis review aimed at presenting the findings of 19 original studies that were published in the period from 2000 to 2022 in order to outline the current state of the art in the field of emotion communication (i.e., expressing and interpreting emotions) in multilingual psychotherapy. This analysis involves in particular the studies that investigate multilingual phenomena (e.g., code‐switching and language‐mixing) that occur during psychotherapeutic sessions when client and therapist can speak more than one language, as well as the articles that research emotion communication in this multilingual setting. With this in mind, the articles selected for this review are divided into two main categories: linguistic phenomena in multilingual psychotherapy and emotion communication in multilingual psychotherapy. Results of the studies in the current analysis reveal that code‐switching remains one of the main linguistic phenomena that arises in the area of multilingual psychotherapy. Switching languages in therapy tends to be considered as beneficial for both client and therapist. Besides, findings suggest a connection between speaking L1/LX and one's identity (e.g., feeling different depending on the language). Additionally, studies that investigate emotion communication in multilingual psychotherapy show that an individual's first language (L1) is predominantly viewed as more emotionally charged than their language(s) learned later in life (LX). However, some studies also demonstrate that the relationship between L1 and LX is more fluid and context‐dependent than it has traditionally been regarded. Propositions are given for future research in the field of emotion communication in multilingual psychotherapy.
Synthesis, Anti‐Fungal Potency and In silico Studies of Novel Steroidal 1,4‐Dihydropyridines
Authors: Khan Asna , Iqbal Arfeen , Ahmedi Saiema , Manzoor Nikhat , Siddiqui Tabassum 
Published: April 2023

AbstractWorking principle of azoles as antifungals is the inhibition of fungal CYP51/lanosterol‐14α‐demethylase via selective coordination with heme iron. This interaction can also cause side effects by binding to host lanosterol‐14α‐demethylase. Hence, it is necessary to design, synthesize and test new antifungal agents that have different structures than those of azoles and other antifungal drugs of choice in clinical practice. Consequently, a series of steroidal 1,4‐dihydropyridine analogs 16–21 were synthesized and screened for their in vitro anti‐fungal activity against three Candida species as steroids‐based medications have low toxicity, less vulnerability to multi‐drug resistance, and high bioavailability by being capable of penetrating the cell wall and binding to specific receptors. Initially, Claisen–Schmidt condensation takes place between steroidal ketone (dehydroepiandrosterone) and an aromatic aldehyde forming steroidal benzylidene 8–13 followed by Hantzsch 1,4‐dihydropyridine synthesis resulting in steroidal 1,4‐dihydropyridine derivatives 16–21. The results exhibited that compound 17 has significant anti‐fungal potential with an MIC value of 750 μg/ml for C. albicans and C. glabrata and 800 μg/ml for C. tropicalis. In silico molecular docking and ADMET studies were also performed for compounds 16–21.
Advances in Halloysite Nanotubes (HNTs)‐Based Mixed‐Matrix Membranes for CO2 Capture
Authors: Rafiq Sikander , Saeed Muhammad , Jamil Asif , Rashid Muhammad Imran , Irfan Muhammad , Iqbal Tanveer , Inayat Abrar , Jamil Farrukh , Iqbal Jibran , Khurram Muhammad Shahzad , Mehadi Muhammad Shozab 
Published: April 2023

AbstractMembrane technology promises a highly economical and efficient solution for CO2 separation. Many polymeric membranes have been reported in the past for the separation of gases specially to remove CO2 from natural gas and low‐pressure flue‐gas streams. The performance of membranes can be tailored by dispersing nanofillers in a polymeric matrix to produce mixed‐matrix membranes (MMMs). This not only adds mechanical strength to membranes but also reduces compaction of the polymeric layer at high pressure and maintains high performance. Halloysite nanotubes (HNTs) gained attention in gas separation technology and due to their tubular structure have been used in a variety of applications in biomedical, coating, composite, and electronic industries. However, very little but conclusive literature and reviews are available to indicate that functionalized and non‐functionalized HNTs can improve the performance of MMMs for efficient CO2 capture. The current status and gaps for potential applications of HNTs‐based membranes for gas separation are identified and reviewed.
Cationic Solution and Solid‐State Emitters – Robust Imaging Agents for Cells, Bacteria, and Protists
Authors: Dubbert Justin , Höing Alexander , Graupner Nadine , Rajter Ľubomír , Dunthorn Micah , Knauer Shirley K. , Galstyan Anzhela , Rizzo Fabio , Voskuhl Jens 
Published: April 2023

AbstractA library of eight different cationic emitters with emission properties in solution and in solid‐state (solution and solid‐state emitters – SSSE) is presented. These compounds, bearing either ammonium or pyridinium groups, have been investigated regarding their photophysical properties as well as their potential application in biological imaging. Besides high quantum yields as well as a high degree of stability during the imaging process, it was additionally revealed that a broad range of biological targets can be addressed, such as different bacterial strains, human cells as well as protists. The reported SSSE approach employing the mentioned robust emitters for biological imaging, will contribute to a rapid and facile way to design and apply affordable emitters with outstanding properties. Additionally, these emitters will overcome the drawbacks of classical luminophores and agents featuring well‐known aggregation‐induced emission (AIE) or aggregation‐caused quenching (ACQ) properties.
Enhanced Performance of CsPbIBr2 Perovskite Solar Cell by Modified Zinc Oxide Nanorods Array with [6,6]‐Phenyl C61 Butyric Acid
Authors: Yang Jien , Zhang Meng , Zhang Qiong , Qin Chaochao , Qin Ruiping , Jain Sagar M. , Liu Hairui 
Published: April 2023

AbstractAlthough Metal oxide ZnO is widely used as electron transport layers in all‐inorganic PSCs due to high electron mobility, high transmittance, and simple preparation processing, the surface defects of ZnO suppress the quality of perovskite film and inhibit the solar cells’ performance. In this work, [6,6]‐Phenyl C61 butyric acid (PCBA) modified zinc oxide nanorods (ZnO NRs) is employed as electron transport layer in perovskite solar cells. The resulting perovskite film coated on the zinc oxide nanorods has better crystallinity and uniformity, facilitating charge carrier transportation, reducing recombination losses, and ultimately improving the cells’ performance. The perovskite solar cell with the device configuration of ITO/ZnO nanorods/PCBA/CsPbIBr2/Spiro‐OMeTAD/Au delivers a high short circuit current density of 11.83 mA cm−2 and power conversion efficiency of 12.05 %.
Exploration of N‐Arylation of Backbone Amides as a Novel Tool for Conformational Modification in Peptides
Authors: Dangi Abha , Kiran Marelli Udaya 
Published: April 2023

AbstractA set of 15 cyclic‐hexaalanine and 10 cyclic‐pentaalanine peptides containing one or two backbone N‐aryl amide bonds were synthesized by following a combination of solution‐phase and solid‐phase peptide synthesis. NMR‐based conformation studies of these N‐aryl cyclic‐hexaalanine peptides revealed five distinct template conformations with an antiparallel β‐sheet structure; for N‐aryl cyclic‐pentaalanine peptides three template structures were revealed. All the template structures have distinct peptide‐turn features. The conformations in these N‐aryl peptides were compared to those in the commonly studied N‐methyl peptide analogues. We observed that the N‐aryl peptides exhibit a considerable conformational homogeneity, and their conformations differ significantly from those in N‐methyl analogues. We anticipate that the N‐arylation of backbone amides has the potential for application as a novel tool for conformation and physicochemical modification in peptides.
Effect of engineered water and scale inhibitor concentration on the adsorption performance of gypsum inhibitors on multi‐walled carbon nanotubes and crushed sandstone
Authors: Hosseini Ali , Talebi Sina , Riahi Siavash 
Published: April 2023

AbstractEngineered waterflooding is an effective process for enhanced oil recovery, but it can lead to mineral scale deposition due to the incompatibility of brines. Previous studies on scale mitigation have mainly focused on high‐temperature baryte and anhydrite scales, neglecting gypsum precipitation and inhibitors adsorption at low temperatures. Additionally, earlier research has used simple brines that do not reflect the actual injection and formation water in reservoirs. In this study, the impact of temperature, various brine mixtures, and thermodynamic databases on saturation ratio and scale precipitation is explored using PHREEQC (pH‐REdox‐EQuilibrium‐C program). The study reveals that the copresence of calcium and magnesium ions improves the gypsum inhibition efficiency of scale inhibitors (SIs) at low concentrations to a maximum of 79%. However, this effect is reversed or neutral at higher SI concentrations. The study also shows that the presence of monovalent ions reduces the adsorption of SIs by multi‐walled carbon nanotubes (MWCNTs). Removing sodium ions from seawater while leaving calcium and magnesium ions intact increases MWCNTs' adsorption capability to 93%. This is because monovalent cations attach to the adsorbent surface and block the active sites, whereas divalent cations act as a bridge between MWCNT and SIs. The study establishes that the behaviour of SIs regarding adsorption on MWCNT and crushed sandstone depends on various factors, including molecule size, calcium toleration of the SIs, point of zero charge, and solution pH. Understanding these factors can improve the effectiveness of SIs, reduce chemical costs, and prolong the life of oil wells.
Reducing unnecessary handovers and improving ranking abnormality based on multi‐attribute decision making graph theory and matrix approach with Euclidean distance in heterogeneous wireless networks
Authors: Kaur Gaganpreet , Goyal Raman Kumar , Mehta Rajesh 
Published: April 2023

SummaryNew mobile devices offer multiple network interfaces to allow the users to connect to the best available network. The heterogeneous networks can provide better internet connectivity to the users by means of vertical handover. The handover must be triggered at a suitable point of time to avoid mobility issues such as unnecessary handovers and handover ping‐pongs. The network selection during handover is usually done using classical multi‐attribute decision making (MADM) methods. However, ranking abnormality is one of the prominent issues of the classical MADM methods. To address these challenges, a graph theory and matrix approach (GTMA) with Euclidean distance is proposed for vertical handover in wireless networks. GTMA is used for the selection of the appropriate network and Euclidean distance is utilized for the handover triggering. The simulation results reveal that the proposed method has eliminated the ranking abnormality issue. This proposed technique without triggering has also reduced the number of handovers up to 75.61%, 85.71%, and 66.67% as compared to the traditional MADM methods such as AHP, GRA, and TOPSIS respectively. The use of Euclidean distance for handover triggering has further reduced the number of handovers of the proposed technique as well as traditional techniques for all the traffic types.