bluuebunny/crossref_metadata_2025_split huggingface dataset:
A call to action to improve the completeness of older adult sexual and gender minority data in electronic health records
Authors: May Jennifer T , Myers John , Noonan Devon , McConnell Eleanor , Cary Michael P 
Published: July 2023

Abstract
Sexual and gender minority (SGM) older adults experience greater health disparities compared to non-SGM older adults. The SGM older adult population is growing rapidly. To address this disparity and gain a better understanding of their unique challenges in healthcare relies on accurate data collection. We conducted a secondary data analysis of 2018–2022 electronic health record data for older adults aged ≥50 years, in 1 large academic health system to determine the source, magnitude, and correlates of missing sexual orientation and gender identity (SOGI) data among hospitalized older adults. Among 153 827 older adults discharged from the hospital, SOGI data missingness was 67.6% for sexual orientation and 63.0% for gender identity. SOGI data are underreported, leading to bias findings when studying health disparities. Without complete SOGI data, healthcare systems will not fully understand the unique needs of SGM individuals and develop tailored interventions and programs to reduce health disparities among these populations.
Effect of ellagic acid on body weight, nutrient digestibility, fecal microbiota, and urolithin A metabolism in Thoroughbred horses
Authors: Li Jiahao , Huang Xinxin , He Linjiao , Li Chao , Jing Hongxin , Lin Jianwei , Ma Chaoyu , Li Xiaobin 
Published: July 2023

Abstract
This study aimed to investigate the effects of ellagic acid (EA) supplementation on body weight (BW), nutrient digestibility, fecal microbiota, blood biochemical indices, and urolithin A metabolism in 1-yr-old Thoroughbred horses. A group of 18 1-yr-old Thoroughbred horses, with an average weight of 339.00 ± 30.11 kg, were randomly allocated into three groups of six horses each (three males and three females). The control group (n = 6) received only the basal diet, whereas test groups I (n = 6) and II (n = 6) were fed the basal diet supplemented with 15 mg/kg BW/d and 30 mg/kg BW/d of EA, respectively, for 40-d. The results showed that test group I and II horses had a significant increase in total weight gain by 49.47% and 62.74%, respectively, compared to the control group. The digestibility of various components in the diets of the test group horses was improved, including dry matter, organic matter, gross energy, neutral detergent fiber, acid detergent fiber, and calcium. Additionally, the digestibility of crude protein and phosphorus (P) in test group II horses increased significantly by 10.96% and 33.56% (P &lt; 0.05), respectively. Moreover, EA supplementation significantly increased the fecal abundance of Firmicutes, Bacteroidetes (P &lt; 0.05), Fibrobacterota, p-251-o5, Desemzia incerta (P &lt; 0.05), and Fibrobacter sp. (P &lt; 0.05), while reducing the abundance of Proteobacteria, Pseudomonadaceae, Pseudomonas, and Cupriavidus pauculus (P &lt; 0.05 or P &lt; 0.01). Fecal samples from test group II showed 89.47%, 100%, and 86.15% increases in the concentrations of acetic acid, valeric acid, and total volatile fatty acids, respectively. In addition, the plasma levels of total protein, and globulin increased significantly in test groups I (7.88% and 11.35%, respectively) and II (13.44% and 16.07%, respectively) compared to those in the control group (P &lt; 0.05). The concentration of urolithin A in fecal and urine samples was positively correlated with increasing doses of EA. These findings suggest that supplemental feeding of EA improved nutrient digestibility, blood biochemical indices, and fecal microbiota in 1-yr-old Thoroughbred horses, promoting growth and development.
Immigration and seasonal bottlenecks: high inbreeding despite high genetic diversity in an oscillating population of Culicoides sonorensis (Diptera: Ceratopogonidae)
Authors: Shults Phillip , Zhang Xinmi , Moran Megan , Cohnstaedt Lee W , Gerry Alec C , Vargo Edward L , Eyer Pierre-Andre 
Published: July 2023

AbstractMost population genetic studies concern spatial genetic differentiation, but far fewer aim at analyzing the temporal genetic changes that occur within populations. Vector species, including mosquitoes and biting midges, are often characterized by oscillating adult population densities, which may affect their dispersal, selection, and genetic diversity over time. Here, we used a population of Culicoides sonorensis from a single site in California to investigate short-term (intra-annual) and long-term (inter-annual) temporal variation in genetic diversity over a 3 yr period. This biting midge species is the primary vector of several viruses affecting both wildlife and livestock, thus a better understanding of the population dynamics of this species can help inform epidemiological studies. We found no significant genetic differentiation between months or years, and no correlation between adult populations and the inbreeding coefficient (FIS). However, we show that repeated periods of low adult abundance during cooler winter months resulted in recurring bottleneck events. Interestingly, we also found a high number of private and rare alleles, which suggests both a large, stable population, as well as a constant influx of migrants from nearby populations. Overall, we showed that the high number of migrants maintains a high level of genetic diversity by introducing new alleles, while this increased diversity is counterbalanced by recurrent bottleneck events potentially purging unfit alleles each year. These results highlight the temporal influences on population structure and genetic diversity in C. sonorensis and provide insight into factors effecting genetic variation that may occur in other vector species with fluctuating populations.
Seasonal distribution of Calliphoridae and Mesenbrinellidae (Diptera) associated with the decomposition of a clothed animal model in a forest reserve in the Central Amazon
Authors: de Souza Alex Sandro Barros , Agra Daniela , Martins Renato Tavares , Gomes Patrícia Pereira , Ferreira-Keppler Ruth Leila 
Published: July 2023

AbstractWe evaluated the effects of seasonality on the richness and abundance of dipterans of the families Calliphoridae and Mesembrinellidae associated with the decomposition of a clothed Large White swine Sus scrofa domesticus(Artiodactyla: Suidae) carcass. Experiments were carried out in less rainy, rainy, and intermediate periods between 2010 and 2011 at Reserva Florestal Ducke, Manaus, Amazonas. Two pig carcasses, each weighing approximately 40 kg, were used in each period. A total of 63,872 individuals of 18 species of Calliphoridae and Mesembrinellidae were collected. The abundance and richness of these dipteran families were influenced by the interaction between period and decomposition stage. The compositions of the Calliphoridae and Mesembrinellidae assemblages differed among periods, with the fauna of the less rainy period being less similar to those of the intermediate and rainy periods than they were to each other. Three species were selected as indicators for the less rainy period, namely Paralucilia pseudolyrcea (Mello, 1969) (Diptera, Calliphoridae), Paralucilia nigrofacialis (Mello, 1969) (Diptera, Calliphoridae), and Eumesembrinella randa (Walker, 1849) (Diptera,Mesembrinellidae) while Chloroprocta idioidea (Robineau-Desvoidy, 1830) (Dipetra, Calliphoridae) was selected as an indicator species for the rainy period; no taxon was selected as an indicator of the intermediate period. Among decomposition stages, only fermentation and black putrefaction had indicator taxa, with Hemilucilia souzalopesi Mello, 1972 (Diptera, Calliphoridae and Chysomya putoria(Wiedemann, 1830) (Diptera, Calliphoridae), respectively. Clothes did not prevent the laying of eggs and became a kind of protection for immature stages. The clothed model presented a delay in decomposition compared to other studies developed in the Amazon region.
From Dadaab Camp to Kismayo City: A Call for Local Evidence to Inform Durable Solutions
Authors: Mohn Charlotte , Tonnarelli Francesco , Weaver Jonathan , Njuguna Winston , Barkhadle Abdirahman 
Published: July 2023

Abstract
These field reflections contribute to the discussion on durable solutions to displacement by providing empirical evidence of how intended spillover effects of carefully designed interventions and hybrid settlements can facilitate local integration and return and reintegration. A comparison between Dadaab and Kismayo reveals humanitarian and development aid’s influence and spillover effects on economic, social, cultural, and political life beyond the borders of refugee camps and returnee settlements. We argue for leveraging such spillover—in particular in the form of hybrid settlements—for achieving durable solutions to displacement cost-effectively and inclusively. Our observations show that several local stakeholders have recognized the potential of spillover, too and are actively embracing it to drive local socio-economic development. Lastly, we highlight the need for further research into the factors that enable or prevent spillover effects and hybrid settlements from reaching their full potential: the achievement of durable solutions for displacement-affected communities.
‘My Family Needed Me’: Exploring Caring Dimensions and Care Circulation among Older Venezuelans on the Move in Peru
Authors: Blouin Cécile , Borios Stéphanie 
Published: July 2023

Abstract
The humanitarian, political, and socio-economic crisis in Venezuela has generated an unprecedented migration to other South American countries. In the last six years, Peru has become the second receptor of Venezuelan people after Colombia and the first regarding asylum seekers. In this article, we follow recent contributions regarding the concept of care circulation to ask: how the case of older Venezuelans on the move illustrates the multidimensionality of care circulation? How these care arrangements can be understood from an age and gender perspective? Through three life stories, we discuss how older Venezuelans on the move practice care for the benefit of the whole family based on family arrangements and negotiations and how, as a result, care circulates in multiple directions and can generate conflicts and power imbalance for the older ones.
(110) EXTRACELLULAR VESICLES ISOLATED FROM PEYRONIE’S DISEASE-DERIVED CELLS CAN ALTER TGF-BETA 1 SIGNALLING CASCADES
Authors: Dr. Marcus M Ilg , Prof. David J Ralph , Prof. Selim Cellek 
Published: July 2023

Abstract

Objectives
The importance of extracellular vesicles (EVs) as key mediators of cell-cell communication has been highlighted in recent years. However, their role in the pathophysiology of Peyronie’s disease (PD) and other fibrotic disorders is still unclear. Fibroblasts and myofibroblasts are key effectors in PD and this study seeks to further elucidate a previously described role of EVs in myofibroblast transformation in PD.

Methods
Primary fibroblasts were isolated from tunica albuginea of PD patients. Cells were cultured in DMEM with 10% Knockout Serum Replacement and EVs were isolated from the conditioned media in presence and absence of transforming growth factor beta 1 (TGF-β1). Isolation of the EVs was performed using ultrafiltration with subsequent size exclusion chromatography. Fibroblast and myofibroblast transcriptome was analysed using Nanostring nCounter. Classical EV markers were confirmed using Western blot. EV quantity, size, and shape was analysed using Tunable resistive pulse sensing (TRPS). Expression of α-smooth muscle actin (α-SMA), pSmad2/3 and pErk1/2 in presence and absence of TGF-β1 was assessed using In-Cell ELISA (ICE).

Results
Myofibroblasts produced more EVs and smaller EVs than fibroblasts and classical EV marker expression was confirmed. Nanostring nCounter revealed that fatty acid metabolism, cholesterol metabolism, and de novo lipogenesis were differentially regulated in cells treated with TGF-β1, in line with increased EV production. Myofibroblast EVs prevented TGF-β1-induced myofibroblast transformation which could be prevented with an inhibitor of vesicle uptake (10 μM dynasore). Myofibroblast-derived EVs but not fibroblast-derived EVs were able to affect downstream effectors of TGF-β1, by preventing the phosphorylation of pErk1/2. No effect on pSmad2/3 phosphorylation was observed.

Conclusions
This is the first study to demonstrate that PD-cell-derived EVs can affect signalling cascades downstream of TGF-β1. Only non-canonical TGF-β1 signalling is influenced and the effect appears dependent on cellular uptake of the vesicles. Further study is needed to decipher the cargo responsible for this effect.

Conflicts of Interest
This study is partly funded by ESSM Research Grant (ESSM RG-19 04).
(137) PENILE LENGTH BEFORE AND AFTER THE IMPLANTATION OF AN INFLATABLE PENILE PROSTHESIS
Authors: Dr. Cedric Jorissen , Dr. Andries Van Huele , Prof. Koenraad van Renterghem 
Published: July 2023

Abstract

Objectives
There is a high satisfaction rate after implantation of an Inflatable Penile Prosthesis (IPP) for patients with erectile dysfunction (ED). The postoperative length of the penis is a factor that can negatively affect this. Therefore, it is important to create realistic expectations. We aim to evaluate the difference in length before and after surgery.

Methods
We analysed data derived from the ongoing PHOENIX trial. Ethics committee registration number B243201940514. We included 100 patients who were operated from 11-2021 to 09-2022, in a single centre by a single surgeon. Only virgin implants were included. Penile length was measured in a standardized way in the flaccid and stretched state preoperatively. After surgery, the length was measured again in deflated and inflated state.

Results
Included patients had a mean age of 61 years. A Coloplast Titan prosthesis was used in 51 patients, an AMS CX prosthesis in 46 patients and an AMS LGX in 3 patients. The mean length of the cylinders was 19.57cm. The average length of the penis after implantation was 7.98cm deflated and 8.36cm inflated. The mean difference in length after implantation between deflated and inflated was 0.3cm. Sub analyses of this parameter to compare the Coloplast and AMS group showed no significant difference (0.26cm vs. 0.48cm respectively). After implantation, the length of the inflated state was on average in all patients, 3.3cm longer than the flaccid state preoperatively, compared to only 0.07cm longer than the stretched state preoperatively. For this also no significant difference between the Coloplast and AMS group.

Conclusions
The stretched penile length preoperatively gives a good estimate of the expected length postoperatively and can be used to give patients a realistic expectation. When the prosthesis is inflated, there is no significant increase in length compared to the deflated state. We found no difference between Coloplast Titan and AMS.

Conflicts of Interest
No conflict of interest.
(267) ERECTILE DYSFUNCTION TREATMENT WITH RADIOFREQUENCY ENERGY-BASED DEVICE RF VERTICA®, 6-MONTH FOLLOW-UP
Authors: Dr. Sergiu Bistritzky , Dr. Boaz Appel , Dr. Arik Shechter , Prof. Ilan Gruenwald 
Published: July 2023

Abstract

Objectives
A recent study of a new radiofrequency-based device (RF Vertica®) in treating Erectile Dysfunction (ED) showed promising results, safety, and satisfaction. However, there are no data on long-term follow-up. We aim to assess 1,3, and 6-months follow-up efficacy based on IIEF-EF and EHS questionnaires.

Methods
Follow-up data assessment of men included in RF energy treatment of ED pilot study. ED was evaluated before the treatment, 1-, 3-, and 6-months following all treatment sessions, using IIEF-EF, Erection Hardness Scale (EHS). Questions addressing side effects and ease of use were also evaluated.

Results
Twenty-seven out of 28 men with ED (mean age 59.5.7±9.8, range: 42-77) completed 1,3, and 6-month follow-ups after concluding all treatment sessions. Mean IIEF-EF and EHS scores significantly improved after ending treatment compared with baseline values, and the treatment was still effective at 3- and 6-months follow-up.
Patients didn't report any difficulties in using the device, and no side effects.

Conclusions
RF Vertica® device is an effective, safe, and easy-to-use method in treating ED. Patients report good treatment response at 1,3-and 6-months follow-ups. There is a need for additional studies with a more significant number of men and longer follow-ups to assess the long-term effects of the treatment.

Conflicts of Interest
Professor Ilan Gruenwald, MD, is a medical consultant at Vertica-Labs company.
(120) PENOSCROTAL DECOMPRESSION SHOULD BE CONSIDERED FOR PROLONGED ISCHEMIC PRIAPISM
Authors: Gin Lee Wai , Dr. Shafi Wardak , Prof. David Ralph 
Published: July 2023

Abstract

Objectives
Outcomes of men with prolonged ischemic priapism (PIP) are poor. Management includes a proximal corporal shunt or immediate penile prosthesis placement with an increased risk of erosion and infection. Penoscrotal decompression (PSD) relieves the acute compartment syndrome of PIP and restores perfusion but experience is limited to 31 cases from North America.

Methods
Retrospective review of men presenting with ischemic priapism confirmed by cavernosal blood gas and biopsy at time of decompression. Duration of ischemia, prior management, clinical outcomes and change in International Index of Erectile Function (IIEF) score were analyzed.

Results
Thirteen patients with a median duration of PIP of 48 hours (IQR 38-56) presented between 2019-2022. Etiology of PIP was idiopathic in 5, iatrogenic or recreational drug-related in 6 and sickle-cell disease (SCD) in 2 men. All failed intracavernosal phenylephrine and 2 failed distal corporo-glanular shunting. All had successful detumescence with 9 (69%) requiring bilateral PSD. Immediate pain relief was achieved in all but one. Another patient with SCD recurred within 24 hours following unilateral PSD that resolved with intracavernosal phenylephrine. Only one man without SCD developed a recurrence of priapism the next day and he proceeded to insertion of a malleable penile prosthesis on day 2. There was no association between unilateral PSD and recurrence. IIEF scores fell by a median of 12 (IQR 2.25-15.5) and 6 men developed refractory erectile dysfunction after median follow-up of 17.6 months (IQR 1.3 – 19.7).

Conclusions
In the first study outside of North America, PSD successfully relieved PIP in all patients with good pain relief for most (n=12, 92%). Six men developed refractory erectile dysfunction while 3 had recurrent priapism due to SCD (n=2) and cocaine abuse (n=1). The lack of fresh corporal bleeding following PSD is a risk factor for failure and an immediate penile prosthesis should be considered. PSD averted the need for penile prosthesis insertion in 54% of men.

Conflicts of Interest
Nil
(130) INGUINAL LYMPHADENECTOMY FOR PENILE CANCER: RESULTS OF A PROSPECTIVE RANDOMIZED TRIAL COMPARING OPEN VS VIDEO LAPAROSCOPIC APPROACH
Authors: Marco Falcone , Dr. Mirko Preto , Dr. Marco Oderda , Dr. Federica Peretti , Dr. Lorenzo Cirigliano , Dr. Ilaria Ferro , Dr. Natalia Plamadeala , Dr. Carlo Ceruti , Prof. Paolo Gontero 
Published: July 2023

Abstract

Objectives
To compare surgical and oncological outcomes between video laparoscopic (VEIL) and open inguinal lymphadenectomy (OIL) in patients affected by locally advanced penile cancer (PC).

Methods
Prospective randomized trial including patients underwent staging or radical inguinal lymphadenectomy for PC from October 2019 to March 2022. Patients were randomized to receive OIL on one groin and VEIL on the contralateral side. Operative time, intra and post-operative complication rate (according to Clavien-Dindo classification), number of removed and positive lymph nodes, disease-free survival (DFS) and overall survival (OS) were collected, compared and analyzed during the follow-up period.

Results
28 inguinal lymphadenectomy procedures satisfied the inclusion and exclusion criteria, 14 (50%) OIL and 14 (50%) VEIL. Median follow-up was 14 months (IQR 6-17). No significant differences in terms of operative times, number of removed lymph nodes, 1-year DFS and 1-year OS were recorded in-between the two surgical approaches. Time to drainage removal was significantly shorter in the VEIL group (15days; IQR 13-17) than in the OIL group (27days; IQR 20-41; p &lt;0.05). No intraoperative complications occurred. Long-term postoperative complications were recorded in 3 (21.4%) and in 8 (57.1%) groins respectively in the VEIL and in the OIL group (p &lt;0.05). In the follow-up period, 2 patients died for PC.

Conclusions
VEIL may reduce postoperative complications when compared to OILL. Further high-powered studies are warranted to confirm these preliminary results.

Conflicts of Interest
None to declare.
(222) THE NEW PENIS SCORING CRITERIA AND PACKAGE CHECKLIST: RESULTS OF A LITERATURE SYSTEMATIC REVIEW OF PENILE AMPUTATION AND REPLANTATION
Authors: Prof Bruno Machado , Aimee Tran , Dr. Nickolas Scherzer , Scott Bailey , Prof. Wayne Hellstrom 
Published: July 2023

Abstract

Objectives
This study has 3 aims: 1) Produce an updated systematic review of penile replantation 2) Appraise the comparative value of the PENIS Score and propose the PACKAGE Checklist for penile amputation case reports and reviews 3) Identify confusing terminology and recommend standardizing vocabulary to describe penile amputation and replantation.

Methods
We assessed 432 full-text case reports in 20 languages identifying 122 microsurgical and 40 surgical cases of penile replantation that qualified for our review. The cases were stratified using the PENIS Score for penile amputations using five criteria: position, extension, neurovascular repair, ischemia time and type, and severed edges and contamination. Four outcomes: postoperative complications, erection, urination, and sensation, were evaluated using Kendall’s tau coefficient for each of the PENIS criteria.

Results
The viability of microsurgical and surgical replantation was equivalent at 92% and 94%, respectively. A statistical correlation was found between microsurgical repair and the return of sensation, though this did not correlate with nerve repair. The return of sensation with nerve repair was 51%, comparable to microsurgical replantation without nerve repair at 42%, both were significantly higher than the 14% found in surgical replantation. A significant correlation was noted between the extension of the amputation and the severity of postoperative complications in microsurgical replantations, (τ = .2649, p = .0126), not observed with surgical replantation. the presence of a skin bridge was associated with a 40% reduction in severe postoperative complications compared to complete amputations, regardless of surgical method.

Conclusions
Microsurgical replantation is superior in the return of sensation, with or without nerve repair. In the absence of microsurgical skills, surgical replantation is an appropriate alternative. The lack of standardization in penile replantation case reports makes the comparison between case-reports difficult. Implementing the PACKAGE Checklist and PENIS Score will help future case reports and systematic reviews, allowing for more robust conclusions.

Conflicts of Interest
Authors do not have conflict of interest.