bluuebunny/crossref_metadata_2025_split huggingface dataset:
Dynamic monitoring of viral gene expression reveals rapid antiviral effects of CD8 T cells recognizing the HCMV-pp65 antigen
Authors: Khan Fawad , Müller Thomas R. , Kasmapour Bahram , Ynga-Durand Mario Alberto , Eiz-Vesper Britta , von Einem Jens , Busch Dirk H. , Cicin-Sain Luka 
Published: July 2023

ABSTRACTHuman Cytomegalovirus (HCMV) is a betaherpesvirus that causes severe disease in immunocompromised transplant recipients. Immunotherapy with CD8 T cells specific for HCMV antigens presented on HLA class-I molecules is explored as strategy for long-term relief to such patients, but the antiviral effectiveness of T cell preparations cannot be efficiently predicted by available methods. Therefore, we developed an Assay for Rapid Measurement of Antiviral T-cell Activity (ARMATA) by real-time automated fluorescent microscopy and used it to study the ability of CD8 T cells to neutralize HCMV and control its spread. As a proof of principle, we used TCR-transgenic T cells specific for the immunodominant HLA-A02-restricted tegumental phosphoprotein pp65. pp65 expression follows an early/late kinetic, but it is not clear at which stage of the virus cycle it acts as an antigen. We measured control of HCMV infection by T cells as early as 6 hours post infection (hpi). The timing of the antigen recognition indicated that it occurred before the late phase of the virus cycle, but also that virion-associated pp65 was not recognized during virus entry into cells. Monitoring of pp65 gene expression dynamics by reporter fluorescent genes revealed that pp65 was detectable as early as 6 hpi, and that a second and much larger bout of expression occurs in the late phase of the virus cycle by 48 hpi. Since transgenic (Tg)-pp65 specific CD8 T cells were activated even when DNA replication was blocked, our data argue that pp65 acts as an early virus gene for immunological purposes. Therefore, ARMATA does not only allow same-day identification of antiviral T-cell activity, but also provides a method to define the timing of antigen recognition in the context of HCMV infection.
Left Behind: Modelling the life expectancy disparities amongst people with disabilities in Low and Middle-Income Countries
Authors: Rotenberg Sara , Smythe Tracey , Kuper Hannah 
Published: July 2023

AbstractObjectiveTo use life tables to model the differences in life expectancy for people with and without disabilities in low- and middle-income countries (LMICs).MethodsWe used data from a recent conducted a meta-analysis of analysis which gathered data from 70 studies to determine Hazard Ratios (HRs) for all-cause mortality by disability status for children 0-15, adults 15-59, and adults 60+, using the World Health Organisation’s definition of disability. To assess the disparities in life expectancy among people with disabilities across 136 low and middle-income countries (LMICs), we constructed life tables using these HRs.gap compared to the population average. These calculations were based on the 2020 United Nations population projections. The life expectancy gap was meta-analysed across countries to calculate the mean difference.FindingsPeople with disabilities in LMIC had a mean life expectancy of 49.3 years (95%C.I. 47.4 – 51.2), compared to 68.5 years (95% C.I. 67.4 – 69.5) for the general population, giving a median gap of 19.2 years (95% C.I. 18.3 – 20.1). The disparity in life expectancy varied across countries, ranging from 9.6 years (95% C.I. 4.7 – 17.4) in Bosnia and Herzegovina to 30.6 years (95% C.I. 16.9 – 40.6) in Nigeria.ConclusionLife expectancy inequities represent an urgent threat to upholding the rights of persons with disabilities and achieving global goals. It is crucial to address the disparities in social determinants of health, and prioritise the inclusion of people with disabilities within health equity efforts to close this gap.
“I told myself, be bold and go and test”: Motivators and barriers to HIV testing among gays, bisexuals, and all other men who sex with men in Ghana – West Africa
Authors: Abu-Ba’are Gamji Rabiu , Aidoo-Frimpong Gloria , Stockton Melissa , Zigah Edem Yaw , Amuah Samuel , Amu-Adu Prince , Amoh-Otoo Richard Panix , Nyblade Laura , Torpey Kwasi , Nelson LaRon E. 
Published: July 2023

AbstractLimited evidence from Ghana and SSA shows that despite consistently a disproportionately high burden of HIV, GBMSM infrequently often delay testing until the point of illness. We therefore used qualitative interviews to collect insights of experiences, motivators, and barriers to HIV testing among GBMSM. Two community-based organizations used snowball and convenience sampling to recruit 10 MSM for IDIs and 8 to 12 for FGDs. We transcribed, coded, identified and analyzed the relationship and commonalities between the participants’ responses. Under experiences with testing, 1) fear of HIV infection created a stressful HIV testing experience; and 2) friendly and supportive healthcare environment facilitated a positive experience in healthcare facilities. Motivators or facilitators of testing include: 1) the perception or belief that HIV testing is a HIV prevention strategy; 2) encouragement from friends and peers; 3) understanding risk associated with certain sexual behaviors such as transactional sex 4) education or information on HIV; 5) access to free testing and incentives; 6) early symptoms and provider recommendation. Barriers to HIV testing include: 1) negative community perceptions of HIV deter; 2) individual-level low risk perception or indifference about HIV infection; 3) location and cost; 4) inadequate testing availability; 5) Stigma at Healthcare facilities. The findings point to the need to address important issues around stigma, education, peer support and healthcare resources through interventions and research to improve HIV testing among GBMSM in the country.
A fast-slow trait continuum at the level of entire communities
Authors: Neyret Margot , Le Provost Gaëtane , Boesing Andrea Larissa , Schneider Florian D. , Baulechner Dennis , Bergmann Joana , Vries Franciska de , Fiore-Donno Anna Maria , Geisen Stefan , Goldmann Kezia , Merges Anna , Saifutdinov Ruslan A. , Simons Nadja K. , Tobias Joseph A. , Zaitsev Andrey S. , Gossner Martin M. , Jung Kirsten , Kandeler Ellen , Krauss Jochen , Penone Caterina , Schloter Michael , Schulz Stefanie , Staab Michael , Wolters Volkmar , Apostolakis Antonios , Birkhofer Klaus , Boch Steffen , Boeddinghaus Runa S. , Bolliger Ralph , Bonkowski Michael , Buscot Francois , Dumack Kenneth , Fischer Markus , Gan Huei Ying , Heinze Johannes , Hölzel Norbert , John Katharina , Klaus Valentin H. , Kleinebecker Till , Marhan Sven , Müller Jörg , Renner Swen C. , Rillig Matthias , Schenk Noëlle V. , Schöning Ingo , Schrumpf Marion , Seibold Sebastian , Socher Stephanie , Solly Emily F. , Teuscher Miriam , van Kleunen Mark , Wubet Tesfaye , Manning Pete 
Published: July 2023

AbstractAcross the tree of life, organismal functional strategies form a continuum from slow-to fast-growing organisms, in response to common drivers such as resource availability and disturbance. However, the synchronization of these strategies at the entire community level is untested. We combine trait data for >2800 above-and belowground taxa from 14 trophic guilds spanning a disturbance and resource availability gradient in German grasslands. Most guilds consistently respond to these drivers through both direct and trophically-mediated effects, resulting in a ‘slow-fast’ axis at the level of the entire community. Fast trait communities were also associated with faster rates of whole ecosystem functioning. These findings demonstrate that ‘slow’ and ‘fast’ strategies can be manifested at the level of whole ecosystems, opening new avenues of ecosystem-level functional classification.
Quantifying Amide-Aromatic Interactions at Molecular and Atomic Levels: Experimentally-determined Enthalpic and Entropic Contributions to Interactions of Amide sp2O, N, C and sp3C Unified Atoms with Naphthalene sp2C Atoms in Water
Authors: Zytkiewicz Emily , Shkel Irina A. , Cheng Xian , Rupanya Anuchit , McClure Kate , Karim Rezwana , Yang Sumin , Yang Felix , Record M. Thomas 
Published: July 2023

AbstractIn addition to amide hydrogen bonds and the hydrophobic effect, interactions involving π-bonded sp2atoms of amides, aromatics and other groups occur in protein self-assembly processes including folding, oligomerization and condensate formation. These interactions also occur in aqueous solutions of amide and aromatic compounds, where they can be quantified. Previous analysis of thermodynamic coefficients quantifying net-favorable interactions of amide compounds with other amides and aromatics revealed that interactions of amide sp2O with amide sp2N unified atoms (presumably C=O···H-N hydrogen bonds) and amide/aromatic sp2C (lone pair-π, n-π*) are particularly favorable. Sp3C-sp3C (hydrophobic), sp3C-sp2C (hydrophobic, CH-π), sp2C-sp2C (hydrophobic, π-π) and sp3C-sp2N interactions are favorable, sp2C-sp2N interactions are neutral, while sp2O-sp2O and sp2N-sp2N self-interactions and sp2O-sp3C interactions are unfavorable. Here, from determinations of favorable effects of fourteen amides on naphthalene solubility at 10, 25 and 45 °C, we dissect amide-aromatic interaction free energies into enthalpic and entropic contributions and find these vary systematically with amide composition. Analysis of these results yields enthalpic and entropic contributions to intrinsic strengths of interactions of amide sp2O, sp2N, sp2C and sp3C unified atoms with aromatic sp2C atoms. For each interaction, enthalpic and entropic contributions have the same sign and are much larger in magnitude than the interaction free energy itself. The amide sp2O-aromatic sp2C interaction is enthalpy-driven and entropically unfavorable, consistent with direct chemical interaction (e.g. lone pair-π) while amide sp3C- and sp2C-aromatic sp2C interactions are entropy-driven and enthalpically unfavorable, consistent with hydrophobic effects. These findings are relevant for interactions involving π-bonded sp2atoms in protein processes.Table of Contents Graphic
Treatment of advanced atherosclerotic mice with the senolytic agent ABT-263 is associated with reduced indices of plaque stability and increased mortality
Authors: Karnewar Santosh , Karnewar Vaishnavi , Shankman Laura S. , Owens Gary K. 
Published: July 2023

AbstractThe use of senolytic agents to remove senescent cells from atherosclerotic lesions is controversial. A common limitation of previous studies is the failure to rigorously define the effects of senolytic agent ABT-263 (Navitoclax) on smooth muscle cells (SMC) despite studies claiming that they are the major source of senescent cells. Moreover, there are no studies of the effect of ABT-263 on endothelial cells (EC), which along with SMC comprise 90% of α-SMA+myofibroblast-like cells in the protective fibrous cap. Here we tested the hypothesis that treatment of advanced atherosclerotic mice with the ABT-263 will reduce lesion size and increase plaque stability. SMC (Myh11-CreERT2-eYFP) and EC (Cdh5-CreERT2-eYFP) lineage tracingApoe-/-mice were fed a WD for 18 weeks, followed by ABT-263 100mg/kg/bw for six weeks or 50mg/kg/bw for nine weeks. ABT-263 treatment did not change lesion size or lumen area of the brachiocephalic artery (BCA). However, ABT-263 treatment reduced SMC by 90% and increased EC-contributions to lesions via EC-to-mesenchymal transition (EndoMT) by 60%. ABT-263 treatment also reduced α-SMA+fibrous cap thickness by 60% and increased mortality by >50%. Contrary to expectations, treatment of WD-fedApoe-/-mice with the senolytic agent ABT-263 resulted in multiple detrimental changes including reduced indices of stability, and increased mortality.Graphical abstractUploaded separately.HighlightsTreatment of Apoe-/- mice with advanced atherosclerosis with the senolytic agent ABT-263 increased mortality by >50%.ABT-263 showed a 90% reduction in SMC but a 60% increase in endothelial cell (EC) contributions to lesions via EC to mesenchymal transition (EndoMT) but prevented adaptive increases in investment of EC-derived cells into the fibrous cap via beneficial EndoMT to myofibroblast transitions that we have shown normally occur when SMC investment into fibrous cap of lesions is impaired.Knock out (KO) of Klf4 in SMC, which results in smaller but more stable atherosclerotic lesions, was associated with reduced expression of pro-senescence markers, but preserved expression of the anti-senescence marker, telomerase reverse transcriptase although it is unclear if the latter is causal or an effect.
Oncostatin M is a Master Regulator of an Inflammatory Network inDnmt3a-Mutant Hematopoietic Stem Cells
Authors: Schwartz Logan S. , Young Kira A. , Stearns Timothy M. , Boyer Nathan , Mujica Kristina D. , Trowbridge Jennifer J. 
Published: July 2023

ABSTRACTAge-associated clonal hematopoiesis (CH) occurs due to somatic mutations accrued in hematopoietic stem cells (HSCs) that confer a selective advantage in the context of aging. The mechanisms by which CH-mutant HSCs gain this advantage with aging are not comprehensively understood. Using unbiased transcriptomic approaches, we identify Oncostatin M (OSM) signaling as a candidate contributor to aging-drivenDnmt3a-mutant CH. We find thatDnmt3a-mutant HSCs from young mice do not functionally respond to acute OSM stimulation with respect to proliferation, apoptosis, hematopoietic engraftment, or myeloid differentiation. However, youngDnmt3a-mutant HSCs transcriptionally upregulate an inflammatory cytokine network in response to acute OSM stimulation including genes encoding IL-6, IL-1β and TNFα. In addition, OSM-stimulatedDnmt3a-mutant HSCs upregulate the anti-inflammatory genesSocs3, Atf3andNr4a1, creating a negative feedback loop limiting sustained activation of the inflammatory network. In the context of an aged bone marrow (BM) microenvironment with chronically elevated levels of OSM,Dnmt3a-mutant HSCs upregulate pro-inflammatory genes but do not upregulateSocs3, Atf3andNr4a1. Together, our work suggests that chronic inflammation with aging exhausts the regulatory mechanisms in young CH-mutant HSCs that resolve inflammatory states, and that OSM is a master regulator of an inflammatory network that contributes to age-associated CH.
Factors affecting users' impulse purchases in online group buying: online consumer reviews, countdowns and self-control
Authors: Sun Jingjing , Li Tingting , Sun Shouqiang 
Published: July 2023

PurposeThis paper aims to investigate how online consumer reviews (OCRs), countdowns and self-control affect consumers' online impulse buying behavior in online group buying (OGB) and uncover the relationship between these factors.Design/methodology/approachBased on the stimulus-organism-response (SOR) framework, this research examines the effects of OCRs, countdowns and self-control on users' impulse purchases. First, the influence of emotions on impulse purchases in group purchasing is investigated. In addition, this study innovatively applies stress-coping theory to group buying research, with countdowns exerting temporal pressure on consumers and OCRs viewed as social pressure, to investigate in depth how countdowns and OCRs affect users' impulse purchase behavior. Finally, this study also surveys the moderating role of users' self-control in the impulse purchase process.FindingsThe results show that the perceived value of OCRs and positive emotions (PE) were positively correlated with impulsiveness (IMP) and the urge to buy impulsively (UBI), while negative emotions (NE) were negatively correlated with IMP. Countdowns (CD) had a positive effect on UBI. Self-control can indirectly affect users' impulse buying by negatively moderating the relationship between PE and UBI, PE and IMP and CD and UBI.Originality/valueThe research results can help group buying platforms and related participants understand the factors influencing users' impulse purchases in OGB and facilitate them to better design strategies to increase product sales.
Supply chain resilience in developing countries: a bibliometric analysis and future research directions
Authors: Kumar Anil , Singh Rohit Kr , Singh Devnaad 
Published: July 2023

PurposeUsing bibliometric, this work aims to evaluate the current status of the body of research on the subject of supply chain resilience (SCR) in developing nations and to provide recommendations for potential lines of inquiry for additional research.Design/methodology/approachThree hundred and thirty-six articles published between 2011 and 2021 were retrieved in Scopus for this bibliometric analysis. This analysis focuses on SCR research conducted in developing countries, highlighting its key authors, countries, institutions, journals, articles and themes.FindingsThis bibliometric review seeks to enrich the discourse on SCR in developing nations through a comprehensive and detailed review of 336 articles covering 138 Journals, 73 countries, 877 authors, 743 organizations and 1,145 author keywords. The United Kingdom, India, and the United States provided a substantial share of the publications. Publication-wise, Hong Kong Polytechnic University and The University of Hong Kong play key roles. The author found that supply chain risk management, sustainability, agile management, artificial intelligence and blockchain are trending topics. Additionally, the author identified eight themes by page rank analysis.Practical implicationsThis study's importance lies primarily in its examination of the current information about SCR in developing countries and significant cluster areas (themes). In the paths, it recommends for further study, which academics may take, and industry professionals should apply in their businesses to create a more resilient and sustainable supply chain.Originality/valueBased on published studies, this study gives exploratory data on SCR in developing countries context. This is first of its kind bibliometric study that focuses on developing countries.
Pet tourism: motivations and assessment in the destinations
Authors: Hidalgo-Fernández Amalia , Moral-Cuadra Salvador , Menor-Campos Antonio , Lopez-Guzman Tomas 
Published: July 2023

PurposeThe purpose of this paper is to perform an analysis of how pets in general and dogs in particular condition the planning and realisation of people’s trips. Similarly, the motivations that lead families to travel with their pets in Spain are analysed.Design/methodology/approachThe methodology used in this research consisted of carrying out fieldwork based on surveys of 1,696 dog owners in Spain. The data analysis was carried out using a Partial Least Squares methodology (PLS-SEM) to test the relationship of the families with their pets, the motivations they have for travelling with their pets and the assessment of the destination by both the family and the pet itself.FindingsThe main results of this research focus on analysing how and when a family plans a trip with their pet, except for their second home, there are several motivations for both families themselves and their pets, which condition this trip and that must be considered by tourist destinations. Similarly, it is also concluded that the motivations are closely related to the attachment that the family has to the pet.Originality/valueThe originality of this research focuses on including the relationship of the family with their dog and the adequacy of the destination to the needs of their pet as motivation to make a certain trip or choose a certain destination.
Trade-offs between accuracy and efficiency in BIM-LCA integration
Authors: Zheng Bowen , Hussain Mudasir , Yang Yang , Chan Albert P.C. , Chi Hung-Lin 
Published: July 2023

PurposeIn the last decades, various building information modeling–life cycle assessment (BIM-LCA) integration approaches have been developed to assess the environmental impact of the built asset. However, there is a lack of consensus on the optimal BIM-LCA integration approach that provides the most accurate and efficient assessment outcomes. To compare and determine their accuracy and efficiency, this study aimed to investigate four typical BIM-LCA integration solutions, namely, conventional, parametric modeling, plug-in and industry foundation classes (IFC)-based integration.Design/methodology/approachThe four integration approaches were developed and applied using the same building project. A quantitative technique for evaluating the accuracy and efficiency of BIM-LCA integration solutions was used. Four indicators for assessing the performance of BIM-LCA integration were (1) validity of LCA results, (2) accuracy of bill-of-quantity (BOQ) extraction, (3) time for developing life cycle inventories (i.e. developing time) and (4) time for calculating LCA results (i.e. calculation time).FindingsThe results show that the plug-in-based approach outperforms others in developing and calculation time, while the conventional one could derive the most accuracy in BOQ extraction and result validity. The parametric modeling approach outperforms the IFC-based method regarding BOQ extraction, developing time and calculation time. Despite this, the IFC-based approach produces LCA outcomes with approximately 1% error, proving its validity.Originality/valueThis paper forms one of the first studies that employ a quantitative and objective method to determine the performance of four typical BIM-LCA integration solutions and reveal the trade-offs between the accuracy and efficiency of the integration approaches. The findings provide practical references for LCA practitioners to select appropriate BIM-LCA integration approaches for evaluating the environmental impact of the built asset during the design phase.
Exploring the paradox of gender preferred leadership in Kenya: a GLOBE study on gender egalitarianism and women in leadership
Authors: Anaya Ella Ruth 
Published: July 2023

Purpose
The purpose of this study is to explore gender gaps, values and practices in a Sub-Saharan African (SSA) country, specifically to identify gender inequality in Kenyan leadership and propose suggestions for advancing gender equity.

Design/methodology/approach
This Kenya study replicated the research design of the Global Leadership and Organizational Behaviour Effectiveness Project for gathering country data. It sampled middle and upper management in the commercial sector (finance and agriculture) and added the civic sectors of health care and education, using quantitative research (267 managers in over 100 organizations) and qualitative research design (30 interviewees from 23 organizations).

Findings
Gender had no apparent effect on leadership attributes nor on preferred modes of leadership. Statistical and thematic analysis revealed conflicting values and behaviours regarding gender equality and leadership. A high cultural dimension score on practices reflects a traditional leadership approach for male dominance, referred to as the Bwana Kubwa model. However, a high value score for gender equality reflects a more contemporary perspective, identified as the Inspirational Idealist. The gender gap presents a cultural paradox and a leadership constraint.

Research limitations/implications
The Inspirational Idealist model advances African leadership theory as it identifies a preferred value-based paradigm of effective performance-based leadership. This is contrary to the prevalent patriarchal practices of the Bwana Kubwa (Big Boss) model, which reflects biased gendered preference.

Practical implications
Male and female leaders need to challenge the status quo and align leadership practices with the articulated cultural values expressed as an Inspirational Idealist model. It has considerable relevance to advancing African leadership theory and informing culturally relevant policies and contextualized practices focused on gender equity in leadership.

Originality/value
This research on practices and values pertaining to gender equality of Kenyan managers is the first in-depth managerial leadership study of any SSA country. It is unique in that it broadened the scope of study to include professional sectors, gender, age and ethnicity variables.