bluuebunny/crossref_metadata_2025_split huggingface dataset:
Simulation of China’s Urban Tourism Activity Based on Improved Density Clustering Algorithm
Authors: Huang Xinyan 
Published: February 2023

Abstract
Tourism is the pillar industry of many cities, and it is also an important key point to promote urban development and maintain urban vitality. At present, the analysis of urban tourism activity in China can better assist the research of regional economic development and promote the orderly development of regional economy. Many scholars have carried out the analysis in this respect. As a new and growing field, artificial intelligence also plays an important role in urban tourism. With the continuous development of science and technology, and the human intelligence field of human research is also developing. New artificial intelligence products continue to emerge. The workload of most artificial intelligence may exceed the manual workload. In order to continuously update artificial intelligence, individuals effectively combine data mining and artificial intelligence, and combine many knowledge disseminated by the network with artificial intelligence technology to create an advanced knowledge network model. This paper uses the OPTICS-based clustering algorithm to analyze the clustering of photos on the Flickr website and obtain information about tourism activities in Chinese cities. With the help of visualization software to visualize the experimental data and verify the experimental results introduced in this article, city tourism activities can be recommended to the destination. At present, many scholars have studied the application of improved density clustering algorithm in the field of biology and image analysis, but there are still some gaps in the development of tourism. This paper can make some contributions to the related fields.
Effective adsorption of Pb (II) from wastewater using MnO2 loaded MgFe-LD(H)O composites: Adsorption behavior and mechanism
Authors: Huang Yongxiang , Luo Xiangping , Liu Chongmin , You Shaohong , Rad Saeed , Qin Litang 
Published: February 2023

Abstract
Pb (II) adsorption by MnO2/ MgFe-layered double hydroxide (MnO2/ MgFe-LDH) and MnO2/ MgFe- Layered metal oxides (MnO2/ MgFe-LDO) materials were experimentally studied in lab-scale batch for remediation property and mechanism analysis. Based on our results, the optimum adsorption capacity for Pb (II) was achieved at the calcination temperature of 400 ℃ for MnO2/ MgFe-LDH. Langmuir and Freundlich adsorption isotherm models, quasi-primary and quasi-secondary kinetics, Elovich model, and thermodynamic studies were used for exploring Pb (II) adsorption mechanism by the two composites. In contrast to MnO2/ MgFe-LDH, MnO2/ MgFe-LDO400℃ have a stronger adsorption capacity, the results show that the Freundlich adsorption isotherm model (R2 > 0.948), the quasi-secondary kinetic model (R2 > 0.998), and the Elovich model (R2 > 0.950) provide great fits to the experimental data, indicating the adsorption of a predominantly chemisorption. The maximum adsorption capacity of MnO2/ MgFe-LDO400°C for Pb (Ⅱ) was 531.86 mg·g− 1 at the dosage of 1.0 g·L− 1, pH of 5.0, and temperature of 25°C. The thermodynamic model suggests that MnO2/ MgFe-LDO400℃ is spontaneously heat-absorbing during the adsorption process. Through characterization analysis, the main mechanisms involved in the adsorption process were include precipitation action, complexation with functional groups, electrostatic attraction, cation exchange and isomorphic replacement, and memory effect. Besides, MnO2/ MgFe-LDO400°C has excellent regeneration ability in five adsorption/desorption experiments. The above results highlight the powerful adsorption capacity of MnO2/ MgFe-LDO400°C and may inspire the development of new types of nanostructured adsorbents for wastewater remediation.
FGF10 Protects against Myocardial Ischemia/Reperfusion Injury through AMPK/mTOR/TFEB Pathway
Authors: Zhu Yu , Hu Zhicheng , Chen Gen , Hu Zhenyu , Zhu Junjie , Ma Hongfang , He Shengqu , Xu Xiejun , Huang Zhiyuan , Dong Yonggan , Cai Yan , Guan Xueqiang , Wang Xu , Wang Yang 
Published: February 2023

Abstract
Myocardial ischemia/reperfusion injury (MIRI) is a common clinical complication that occurs upon reperfusion therapy for acute myocardial infarction. Recent studies indicated that impaired autophagic flux contributes to MIRI-induced apoptosis of cardiomyocytes (CMs). Fibroblast growth factor 10 (FGF10), a multifunctional FGF family member, was reported to protect against renal and hepatic ischemia/reperfusion injury. However, it has not been investigated whether FGF10 has a similar beneficial effect against MIRI, and if so, whether autophagy is involved in this effect. Herein, we found that FGF10 was upregulated in mice with MIRI and neonatal rat cardiomyocytes (NRCMs) with hypoxia/reoxygenation injury. FGF10 treatment decreased infarct size and improved cardiac function upon MIRI. FGF10 attenuated MIRI-induced apoptosis of CMs and impairment of autophagic flux mainly through the AMPK/mTOR/TFEB pathway. Therefore, FGF10 may have potential applications for the prevention and treatment of MIRI.
Diagnostic yield and novel candidate genes by exome sequencing in 166 children with intrahepatic cholestasis
Authors: Zheng Yucan , Guo Hongmei , Chen Leilei , Chen Weixia , Yan Kunlong , Zhang Zhihua , Li Mei , Jin Yu , Hu Guorui , Wang Chunli , Zhou Chunlei , zheng bixia , Zhou Wei , Jia Zhanjun , Liu Zhifeng 
Published: February 2023

Abstract
Background & Aims:
Cholestatic liver disease is a leading referral to pediatric liver transplant centers. Inherited disorders are the second most frequent cause of cholestasis in the first months of life.
Methods:
We retrospectively characterized the genotype and phenotype of 166 participants with intrahepatic cholestasis，and re-analyzed phenotype and WES data from patients with previously undetermined genetic etiology for newly published genes and novel candidates. Functional validations of selected variants were conducted in cultured cells.
Results:
Overall, we identified disease causing variants in 31% (52/166) of our study participants. Of the 52 individuals, 18 (35%) were metabolic liver diseases, 9 (17%) were syndromic cholestasis, 9 (17%) were progressive familial intrahepatic cholestasis, 3 (6%) were bile acid synthesis defects, 3(6%) were infantile liver failure and 10 (19%) were phenocopy of intrahepatic cholestasis. By reverse phenotyping, we identified a de novo variant c.1883G>A in FAM111B of a case with high glutamyl transpeptidase (GGT) cholestasis. By reanalyzing WES data, two patients were newly solved, which had compound heterozygous variants in recently published genes KIF12 and USP53, respectively. Our additional search for novel candidates in unsolved trio families revealed four potential novel candidate genes (NCOA6, CCDC88B, USP24 and ATP11C), among which the patients with variants in NCOA6 and ATP11C recapitulate the cholestasis phenotype in mice model.
Conclusions:
In a single center pediatric cohort, we identified monogenic variants in 22 known human intrahepatic cholestasis or phenocopy genes, explaining up to 31% of the intrahepatic cholestasis patients. Rigorous analysis of WES data of well-phenotyped patients with intrahepatic cholestasis leads to a broader understanding of gene-specific phenotypic spectra as well as monogenic candidate gene identification.
Bortezomib, Lenalidomide and Dexamethasone (VRd) vs Carfilzomib, Lenalidomide and Dexamethasone (KRd) as Induction Therapy in Newly Diagnosed Multiple Myeloma
Authors: Tan Carlyn Rose , Derkach Andriy , Nemirovsky David , Ciardiello Amanda , Diamond Benjamin , Hultcrantz Malin , Hassoun Hani , Mailankody Sham , Shah Urvi , Maclachlan Kylee , Patel Dhwani , Lahoud Oscar , Landau Heather , Chung David , Shah Gunjan , Scordo Michael , Giralt Sergio , Lesokhin Alexander , Usmani Saad , Landgren Ola , Korde Neha 
Published: February 2023

Abstract
Lenalidomide and dexamethasone with bortezomib (VRd) or carfilzomib (KRd) are commonly used induction regimens in the U.S. This single-center, retrospective study evaluated outcomes and safety of VRd and KRd. Primary endpoint was progression-free survival (PFS). Of 389 patients with newly diagnosed multiple myeloma, 198 received VRd and 191 received KRd. Median PFS was not reached (NR) in both groups; 5-year PFS was 56% (95%CI, 48%-64%) for VRd and 67% (60%-75%) for KRd (P = 0.027). Estimated 5-year EFS was 34% (95%CI, 27%-42%) for VRd and 52% (45%-60%) for KRd (P < 0.001) with corresponding 5-year OS of 80% (95%CI, 75%-87%) and 90% (85%-95%), respectively (P = 0.053). For standard-risk patients, 5-year PFS was 68% (95%CI, 60%-78%) for VRd and 75% (65%-85%) for KRd (P = 0.20) with 5-year OS of 87% (95%CI, 81%-94%) and 93% (87%-99%), respectively (P = 0.13). For high-risk patients, median PFS was 41 months (95%CI, 32.8–61.1) for VRd and 70.9 months (58.2-NR) for KRd (P = 0.016). Respective 5-year PFS and OS were 35% (95%CI, 24%-51%) and 69% (58%-82%) for VRd and 58% (47%-71%) and 88% (80%-97%, P = 0.044) for KRd. Overall, KRd resulted in improved PFS and EFS with a trend toward improved OS compared to VRd with associations primarily driven by improvements in outcome for high-risk patients.
Understaning the tip-growth and root-growth vapor-liquid-solid mechanism in SnO2 nanowires by Comsol simulation
Authors: Nguyen Hoang Hai , Nguyen Minh Hieu 
Published: February 2023

Abstract
The conventional vapor-liquid-solid (VLS) mechanism has been widely accepted for understanding the tip-growth process of many metal nanowires. However, that model should be more comprehensive to explain the growth process of metal oxide. In this article, we produce tin oxide nanowires with gold nanoparticles and gold film as catalysts, which follow a tip-growth and root-growth process. Experimental results, such as transmission electron microscope images, X-ray photoelectron spectroscopy, and X-ray diffraction patterns, demonstrate that the tip-growth process occurs on Au nanoparticles, and the root-growth process appears on Au thick film. Both methods can be observed when nanowires develop in the presence of both Au nanoparticles and Au film. In this case, the size and length of root-growth nanowires are much larger than that of tip-growth nanowires. For example, the length of the earlier is about 2 micrometers, whereas that of the latter ranges from 20 to 100 micrometers. The irregular shape of particles on the tip of nanowires is very different from other studies, which are usually spherical droplet-like. This suggests a VLS mechanism where there is the existence of partly melted particles playing the role of catalyst. The explanation based on the Au-Si and Au-Sn phase diagram reveals an essential role of the wettability of tin oxide to the Au-Sn alloy liquid. The non-wetting property of tin oxide is necessary for the tip-growth VLS and the root-growth VLS mechanism. Comsol simulation allows calculating the concentration and the flux of Sn atoms arriving on the Au-Sn surface. It suggests that long nanowires in the samples come from both tip-growth and root-growth processes.
NGS-based targeted sequencing identified eight novel variants in patients with Duchenne muscular dystrophy from southwestern China
Authors: Tang Feng , Xiao Yuanyuan , Zhou Cong , Zhang Haixia , Jing Wang , Zeng Yang 
Published: February 2023

Abstract
Background: At present, Multiplex ligation-dependent probe amplification (MLPA) and exome sequencing (ES) are common gene detection methods in patients with Duchenne muscular dystrophy (DMD), but they can not cover the whole genome sequence of DMD gene. In this study, the whole-genome capture of DMD gene and next-generation sequencing (NGS) technology were used to detect the patients with DMD in Southwest China, so as to clarify the application value of this technology and further study the gene mutation spectrum.
Methods: From 2017 to 2020, 51 unrelated DMD patients in southwestern China were clinically diagnosed in West China Second University Hospital of Sichuan University (Chengdu, China). The whole-genome of the DMD gene was captured from the peripheral blood of all patients, and next-generation sequencing was performed. Large copy number variants (CNVs) in the exon region of the DMD gene was verified through MLPA, and small variations (such as single nucleotide variation and < 50 bp fragment insertion/deletion) were validated using Sanger sequencing.
Results: Among the 51 patients, 49 (96.1%) had pathogenic or likely pathogenic variants in the DMD gene. Among the 49 positive samples, 17 patients (34.7%) had CNVs in the exon region and 32 patients (65.3%) had small variations. A total of eight novel variants were identified: c.10916_10917del, c.1790T>A, c.1842del, c.2461G>T, c.3856G>T, c.5015del, c.5791_5792insCA, and exons 38-50 duplication.
Conclusions: Pathogenic or likely pathogenic variants of DMD gene were detected in 49 patients (96.1%), of which 8 variants (16.3%) had not been previously reported. This study not only confirmed the value of NGS-based targeted sequencing for DMD gene but also expanded the gene mutation spectrum of DMD, which may provide effective genetic counseling and prenatal diagnosis for the families.
Perils of irrigated agriculture in urban environment: case study from the Mumbai Metropolitan Region (MMR), India
Authors: Vazhacharickal Prem Jose , Chandrasekhar Trupti , Chandrasekharam D. 
Published: February 2023

Abstract
The role of urban and peri-urban agriculture (UPA) role is to reduce urban poverty and ensure urban food security, income generation, and livelihood strategies that support Millennium Development Goals (MDGs). The UPA production systems often use intensive management techniques and untreated wastewater for food production. The use of untreated sewage may challenge the quality of food products and pose health hazards through the food chain. Quantitative data about the Phyto-availability and food chain transfer of heavy metals in the Mumbai Metropolitan Region (MMR) is scarce. This study was conducted to characterise the elemental and heavy metal transfer among major UPA production systems (farms and railway gardens), in MMR eliciting the soil to root translocation as well as its localisation in produce. It comprises a detailed two-year onsite examination of three farms (F1-3) and three railway gardens (RG1-3) across MMR. Potential risk assessments have been conducted by metal transfer factor (MTF), metal translocation (TF), daily intake of metals (DIM), health risk index (HRI), the average daily dose (ADD) and target hazard quotient (THQ), as well as total metal and element content in comparison with different safety standards. Copper concentration in soils ranged from 29.7 - 545 mg kg-1, with the highest and lowest concentrations observed at RG3 and RG2, respectively. The shoots of white radish accumulated Sr concentrations up to 424 mg kg-1 at RG2. Strontium had a TF up to 32.3 compared with Co, Cu, Zn, Ni and Cr with a maximum of 5.9, 5.3, 3.4, 1.7 and 1.5, respectively. The average daily dose of Zn was between 1.3 × 10-1 and 3.6 × 10-1 mg kg-1 d-1 while Ni had a daily dose of between 4.6 × 10-2 and 7.4 × 10-2 mg kg-1 d-1. The estimated values of ADD were below the world standard levels except for Cr and Ni, and there is a relative absence of health risk imposed by the ingestion of these vegetables produced in UPA systems in MMR. Detailed investigations are needed to identify the sources, mobilisation of heavy metals and trace element contaminations.
Ankrd11, a chromatin regulator and a KBG syndrome risk gene, is a critical regulator of cardiac neural crest cell biology and heart development
Authors: Kibalnyk Yana , Noble Ronan , Alexiou Maria , Poverennaya Irina , Dittmann Nicole , Greenwell Amanda , Goodkey Kara , Ussher John , Adameyko Igor , Graf Daniel , Bourque Stephane , Voronova Anastassia 
Published: February 2023

Abstract

ANKRD11 (Ankyrin Repeat Domain 11) is a chromatin regulator and a risk gene for KBG syndrome, a rare developmental disorder characterized by multiple organ abnormalities, including cardiac defects. However, the role of ANKRD11 in heart development is unknown. The neural crest plays a leading role in embryonic heart development, and its dysfunction is implicated in many congenital heart defects. Here, we demonstrate that conditional knockout of Ankrd11 in the murine embryonic neural crest leads to a severe congenital cardiac defect termed persistent truncus arteriosus (PTA), ventricular dilation, and impaired ventricular contractility. We further show these defects occur due to aberrant cardiac neural crest cell organization and failure to initiate outflow tract septation. Finally, conditional knockout of Ankrd11 in the neural crest leads to impaired Sema3C (Semaphorin 3C) expression, and reduced mTOR (mammalian target of rapamycin) and BMP (Bone Morphogenetic Protein) signaling in the cardiac neural crest cells within the outflow tract. This study identifies Ankrd11 as a novel regulator of neural crest-mediated heart development and function and suggests a mechanism for aberrant heart development in KBG syndrome patients.
Experimental study on sewage collection and related performance of square arc angle aquaculture tank
Authors: Che Zonglong , Zhou Yinxin , Zhao Chenxu , Ren Xiaozhong , Liu Hangfei , Sun Wei , Liu Haibo , Li Meng 
Published: February 2023

Abstract

In order to study the influence of the diameter to depth ratio of the square arc angle aquaculture tank on the flow field and sewage discharge characteristics in the aquaculture tank, a reduced physical model test system of the aquaculture tank with single-drain sewage discharge structure was established based on the actual size of the recirculating aquaculture tank. Under the conditions of different positions of the inlet pipe (single inlet pipe set at arc wall, double pipe set at opposite arc wall), the number of daily water cycle times (40 times, 60 times), the inflow angle (0°, 45°) and other parameters, the influence of the variation of diameter to depth ratios (2:1~7:1) (that is, the ratio of the length of the tank to the depth of water) on the flow field characteristics and sewage discharge effect was systematically analyzed. The results show that the appropriate diameter to depth ratio is the key to improve the sewage discharge efficiency in the tanks. The smaller the diameter to depth ratio is, the higher the sewage discharge efficiency is. When the diameter to depth ratios of the tanks with square arc angle are 2:1~3:1, the sewage discharge efficiency is good. When the diameter to depth ratio are 3.5:1~4.5:1, the sewage discharge effect is better. When the diameter to depth ratio are 5:1~7:1, the sewage discharge effect is weakened greatly. The self-purification capacity of aquaculture tanks and the discharge efficiency of sewage can be improved by choosing the proper diameter to depth ratio, increasing the daily water cycle times times and adjusting the placement of inlet pipes and inflow angle according to the actual needs. The the flow field characteristics in the aquaculture tank are closely related to the distribution and discharge efficiency of sewage. The high uniformity of the flow velocity is conducive to the accumulation of sewage. And the higher the flow velocity is, the higher the discharge efficiency is. The research results can provide theoretical basis and reference for the optimal design of diameter to depth ratio of square arc angle aquaculture tank.
Left or right, that is the question: Use of egocentric frame of reference and the right-eye advantage for understanding gestural signs in bottlenose dolphins (Tursiops truncates)
Authors: Tomonaga Masaki , Uwano-Ito Yuka , Saito Toyoshi , Sakurai Natsuko 
Published: February 2023

Abstract

How do bottlenose dolphins visually perceive the space around them? In particular, what coordinates do they use as a frame of reference for left-right perception? To address this question, we examined the dolphin's responses to various manipulations of the spatial relationship between the dolphin and the trainer by using gestural signs for actions given by the trainer, which have different meanings in the left and right hands. When the dolphins were tested with their backs to the trainer (Experiment 1) or in an inverted position underwater (Experiments 2 and 3), correct responses from the trainer's perspective were maintained for signs related to movement direction instructions. In contrast, reversed responses were frequently observed for signs that required different sounds for the left and right hands. When the movement direction instructions were presented with symmetrical graphic signs such as "×" and "●", accuracy decreased in the inverted posture (Experiment 3). Furthermore, when the signs for sounds were presented from either the left or right side of the dolphin's body, performance was better when the side of the sign movement coincided with the body side on which it was presented than when it was mismatched (Experiment 4). In the final experiment, when one eye was covered with an eyecup, the results showed that, as in the case of body-side presentation, performance was better when the open eye coincided with the side on which the sign movement was presented. These results indicate that dolphins used the egocentric frame for visuospatial cognition. In addition, they showed better performances when the gestural signs were presented to the right eye, suggesting a left-hemispheric advantage in the dolphin's visuospatial cognition.
Novel fatty acid metabolism biomarkers of immunotherapy response in cervical cancer
Authors: Ran Zhihong , Zhang Lei , Chen Lulu , Dong Ming , Song Qibin 
Published: February 2023

Abstract
Fatty acid metabolism abnormalities played an important role in cervical cancer, and current tumor stage has entered the molecular era, which determined the genomic characteristics and prognosis of cancer more precisely than the traditional TNM stage. However, molecular typing on cervical cancer based on fatty acid metabolism has not yet been unclear. Here we downloaded the gene set of fatty acid metabolism from the MSigDB database and classified cervical cancer into three independent genomic stage types-C1, C2 and C3, by extracting the expression in TCGA. Kaplan-Meier survival analysis showed significant survival differences among the three (p < 0.05), and MCPcounter analysis showed that CD8+ T-cell infiltration was more in C3, which had the best prognosis. The MCPcounter analysis showed more CD8+ T-cell infiltration in the C3 type with the best prognosis and more fibroblast infiltration in the C1 type with the worst prognosis (p < 0.05). WGCNA analysis was performed on the three molecular typologies to identify the best correlated blue modules, identify the co-expressed genes in which the association was greater than 0.3, and take the intersection with the differential genes of the three molecular typologies. A novel prognostic model for fatty acid metabolism genomics was developed. Survival analysis showed better survival differences in this model with higher immune and stromal scores in the low-risk group. 31 genes in the model were negatively correlated with immune checkpoints overall. For immunotherapy efficacy analysis, the immunotherapy efficacy was higher in the low-risk group than in the high-risk group in IPS score, and the immune dysfunction level was higher in the low-risk group than in the high-risk group in the TIDE algorithm, whereas the immune escape ability was higher in the high-risk group than in the low-risk group, and the immunotherapy level was higher in the high-risk group than in the low-risk group overall (p < 0.05). Mechanistically, the high-risk group was mainly enriched in the pathways of intercellular interaction, cell-matrix remodeling, angiogenesis, and epithelial-mesenchymal transition. In conclusion, the prognostic model of cervical cancer constructed based on the molecular typing of fatty acids metabolism could predict the prognosis and immunotherapy of the patients with cervical cancer.