bluuebunny/crossref_metadata_2025_split huggingface dataset:
Hypoxic Macrophage-derived Exosomes Mediate Colorectal Cancer Progression through Targeting the Hippo Signaling Pathway
Authors: Jiang Jiang , Wang Wenfang , Zhu Lan , Shi Bowen , Chen Yong , Xia Yihan , Feng Weiming , Yao Weiwu , Lu Aiguo , Zhang Huan 
Published: January 2023

Abstract
Background
The crosstalk between tumor cells and macrophages under hypoxia contributes to colorectal cancer (CRC) progression. Playing an important role in tumor growth by promoting macrophages to polarize to M2-like macrophages, hypoxic tumor cell-derived exosomes have been reported to be important mediators in this crosstalk. However, whether hypoxic macrophage-derived exosomes (HMDEs) modulate CRC progression remains unknown.
Methods
Exosomes from cell culture medium were separated, quantitated, and verified by transmission electronic microscopy and nanoparticle tracking analysis (NTA). Western blotting, real-time quantitative PCR, luciferase reporter assay, Co-immunoprecipitation, chromatin immunoprecipitation, Cell Counting Kit-8 assay, flow cytometry, and immunofluorescence staining were employed to explore the mechanisms by which HMDEs in regulate CRC development under hypoxia. BALB/c-nu/nu mice were utilized to verify CRC proliferation in vivo.
Results
Hypoxia promoted exosome release from macrophages, which could contribute to CRC progression by enhancing cell-cycle transition and inhibiting cell apoptosis. In the context of hypoxia, Hif-1α was highly overexpressed and it could directly bind to the − 521‒ -516 bp and − 401‒ -391 bp regions of the Hsp90 promoter, regulating Hsp90 expression and leading to high levels of Hsp90 protein in HMDEs. Binding to Lats1, Hsp90 inactivated Lats1 and inhibited Yap phosphorylation so as to inactivate the Hippo signaling pathway which was responsible for HMDEs-mediated CRC growth in vivo and in vitro.
Conclusions
We have demonstrated that hypoxic macrophage-derived exosomal Hsp90 induced CRC progression by inactivation of the Hippo signaling pathway, which promoted CRC cell proliferation. Therefore, our study provides a novel understanding of the crosstalk between macrophages and CRC cells under hypoxia.
Manifestation and Sociodemographic Microdata of Brazil's Unified Health System Ombudsman
Authors: Saldanha Raphael de Freitas , Kreischer Vinicius , Gritz Raquel , Miloski Matheus , Bonifácio Carmen Lúcia Corrêa , Cardoso Carlos Leonardo Souza , Alves Ariane Camilo Pinheiro , de Sá Domício Aurélio , Junior Garibaldi Dantas Gurgel , Moreira Rafael da Silveira , Fernandez Michelle Vieira , Lima Jefferson da Costa , Pedroso Marcel 
Published: January 2023

Abstract
Objectives: This article presents the process of extraction and treatment of two datasets from the General Ombudsman of the Brazilian Unified Health System (OUVSUS). The resulting datasets allow the analysis of manifestation characteristics and sociodemographic profile of the citizens that performed these manifestations. Data description: The first dataset depicts the characteristics of the manifestations registered by the General Ombudsman. Each row represents an individual manifestation and contains information such as the registration date, classification, input channel, and subject, among others. The second dataset is constituted of sociodemographic information for each citizen that performed a manifestation, and characteristics such as sexual orientation, race, age, and geographic location of the citizen are presented, among others.
Collagen I is expressed by hepatocytes through activation of AIM2/ASC/caspase-1 signaling pathway
Authors: Sheng Qiongyu , Zhai Panpan , Chen Luanluan , Zhou Liyang , Ou Xueting , Situ Bing , Huang Jun , Pan Xingfei 
Published: January 2023

Abstract
Background
Liver fibrosis is the result of diffuse excessive deposition of extracellular matrix (ECM) in liver. Collagen is the main component of extracellular matrix. Absent in melanoma 2 (AIM2) is involved in the formation of inflammsome and plays an important role in inflammatory response. However, it is unclear whether AIM2 is involved in the pathogenesis of liver fibrosis. In the present study, we explored the role of AIM2 in the expression of collagen I.
Methods
In this study, AIM2 was used to co-culture with HepG2 cells. Cell counting kit-8 (CCK-8) was used to measure cell viability. Real time-quantitative PCR (RT-qPCR) and Western blotting were used to detect collagen I expression at mRNA or protein level, respectively. Then HepG2 cells were treated with caspase activation recruitment domain (ASC), pcDNA(+)-AIM2, small interfering RNA (siRNA) and Z-YVAD-fluoromethylketone (Z-YVAD-FMK) to explore their roles in collagen I expression, respectively.
Results
The viability of HepG2 cells could be not affected with the increased concentrations of AIM2 and Z-YVAD-FMK. The filamentous prisms and vacuoles of HepG2 cells became more obvious when the concentrations of AIM2 increased to 80ng/ml. The expression level of collagen I increased with the increased concentrations of AIM2. The expression level of collagen I could be also induced by pcDNA(+)-AIM2 vector. The expression level of collagen I could be inhibited by ASC siRNA and Z-YVAD-FMK, respectively.
Conclusion
Collagen I expression could be induced by AIM2 through ASC/caspase-1 signaling pathway. AIM2 might be involved in the pathogenesis of liver fibrosis through inducing collagen I expression.
The Distributed Antenna System and Wireless Positioning Mechanism Based on Optimized Radio-Over-Fiber technology
Authors: Ji Wei , Yun Hao , Shi XiangYuan , Yin Peng 
Published: January 2023

Abstract
In this paper, a radio-over-fiber distributed antenna system (ROF-DAS) and wireless positioning mechanism are proposed. The modified Medium Access Control (MAC) frame, optical switch array, ROF network based on star topology and Reflective Semiconductor Optical Amplifier (RSOA) are employed to design the system. A Sigma Delta Modulator (SDM) is employed in the system to optimize the performance of signal transmission. Furthermore, based on the structure, wireless positioning could be achieved by Time Division Multiplexing-Time Difference OF Arrival (TDM-TDOA) technology combined with Chan assisted Taylor algorithm. The improvement of signal transmission quality using SDM is performed by Q Factor, and a comparison of positioning accuracy is performed in terms of the antennas amount using the positioning algorithm.
Isolated unilateral brachial plexus injury following carbon monoxide intoxication: a case report
Authors: liu Shu , Sun Hou-chao , Yang Xu , Cai Zhi-You 
Published: January 2023

Abstract
Background
CO is an odorless, colorless gas that often still undetectable until exposure results in coma or death. Carbon monoxide (CO) intoxication is a common fatal poisoning worldwide. The toxic effects of CO lead to tissue hypoxia and produce systemic and neurological complications. Besides, some severe neurological manifestations and delayed sequelae may occur after CO intoxication. However, peripheral neuropathy following CO poisoning is relatively rare that usually occurs in young people. Beforehand, only one case reported unilateralplexopathy following CO poisoning, which combined with rhabdomyolysis and cognitive dysfunction.
Case presentation
A 31-year-old young man slept in his private car with closed doors and windows. His wife discovered him in about 7 hours when he was in an unconscious state. He was diagnosed with acute carbon monoxide poisoning. After about 12 hours in a coma, he returned to an alert state without conscious disorder. However, he woke up with left upper arm weakness associated with limitation of movement of the left upper limb. The electrophysiological conclusion was left incomplete total brachial plexopathy.
Conclusion
Ischemia of spinal cordcaused by CO intoxication may be a key mechanism in this case. Hyperbaric oxygen therapy as soon as possible is necessary to prevent peripheral neuropathy after acute CO intoxication. Besides, persisting in rehabilitation training might be important to treat peripheral neuropathy after acute CO intoxication.
M2 macrophages are the origin of tumor metastasis
Authors: Li Hui , Miao Yingqi , Suo Liping , Wang Xi , Mao Yiqing , Zhang Xuehui , Zhou Na , Tian Junrui , Yu Xiuyan , Wang Tongxia , Gao Yan , Guo Hongyan , Zhang Zheng , Ma Diansheng , Wu Hongxia , Cui Yanwei , Zhang Xiliang , Chi Xiaochun , Li Yuchi , Irwin David , Niu Gang , Tan Huanran 
Published: January 2023

Abstract
Tumor metastasis is a key factor affecting the life of patients with malignant tumors. For the past hundred years, scientists have been focusing on how to kill cancer cells and inhibit their metastasis in vivo, but few breakthroughs have been made. Here we propose a novel mode for cancer metastasis. Here we show that the phagocytosis of apoptotic tumor cells by macrophages leads to their polarization into the M2 phenotype, and that the expression of stem cell related as well as drug resistance genes are induced. Therefore, it appears that M2 macrophages have "defected" and have been transformed into the initial "metastatic cancer cells", and thus are the source of the distal tissue tumor metastasis. This conclusion is supported by the presence of fused cells with both macrophage and tumor cell characteristics in the peripheral blood and ascites of patients with ovarian cancer. By suppressing the expression of CD206 in M2 macrophages by siRNA, we show that the growth and metastasis of tumors is suppressed at the in vitro cell line and in the in vivo experimental mice models. In summary, we show that M2 macrophages in the blood circulation undergo a "change of loyalty" to become "cancer cells" that undergo distal tissue metastasis, which can be suppressed by the knockdown of CD206 expression.
On scalarization and well-posedness in set optimization with a partial set order relation
Authors: Gupta Sakshi , Gupta Rekha , Srivast Manjari 
Published: January 2023

Abstract
In this paper, a new scalarization function is introduced with respect to a partial set order relation established by Karaman et. al. (Positivity 22(3):783-802,2018 ). A few properties of this function are studied. Scalarization results and some characterizations for set of minimal and weak minimal solutions of a set optimization problem (SOP) in terms of the optimal solution set of the scalar optimization problem (P) are obtained using the newly defined scalarization function. Further, two types of well-posedness for (SOP) are introduced. Equivalence between the well-posedness of (SOP) with (P) is established and a few necessary conditions are obtained for the two well-posedness defined above.
Mathematics Subject Classification (2010) 49J53 · 49K40 · 90C48
Impact of congenital heart disease on outcomes among pediatric patients hospitalized for COVID-19 infection
Authors: Ghimire Laxmi V. , Chou Fu-Sheng , Aljohani Othman A. , Moon-Grady Anita J. 
Published: January 2023

Abstract
Background
COVID-19 infection is generally regarded as an acute self-limiting illness in children, but it can cause significant morbidity and mortality in both healthy and high-risk children. There are limited data on the outcomes of children with congenital heart disease (CHD) and COVID-19. This study aimed to examine the risks of mortality, in-hospital cardiovascular and non-cardiovascular complications in this patient population.
Methods
We analyzed data from hospitalized pediatric patients from 2020 using the nationally representative National Inpatient Sample (NIS). Children hospitalized for COVID-19 were included, and weighted data were used to compare in-hospital mortality and morbidities between children with and without CHD.
Results
Out of 33,220 children admitted with a diagnosis of COVID-19 infection(ICD-10 code:U07.1) during calendar year 2020, 875 (2.6%) had CHD. Compared to children without CHD, children with CHD had similar in-hospital mortality (1.2% vs 0.8%, p = 0.63), with adjusted OR (aOR) of 2.0 (95% CI: 0.5–8.3). Tachyarrhythmias and heart block were more likely in CHD children with an aOR of 4.9 (95% CI: 1.9–12.4) and aOR of 4.4 (95% CI: 2.0-9.7), respectively. Similarly, respiratory failure [aOR = 1.8 (1.2–2.9)], respiratory failure requiring non-invasive mechanical ventilation [aOR = 3.1 (1.5–6.2)] and invasive mechanical ventilation [aOR = 2.2 (1.2-4.0)], and acute kidney injury [aOR = 3.0 (1.8–4.9)] were all significantly higher among patients with CHD. Median length of hospital stay in children with CHD was longer than those without CHD [5 days (IQR: 2-9.3) vs. 3 days (IQR: 2–5), p = < 0.001].
Conclusions
Children with CHD hospitalized with COVID-19 infection were at increased risk of serious cardiovascular and non-cardiovascular adverse clinical outcomes. They were not at increased risk for death when compared to children without CHD but had increased length of hospital stay and utilization of healthcare resources.
In silico characterization of Tomato Leaf Curl New Delhi Virus in Manipur: Evidence for its global expansion and future threat
Authors: Chakraborty Swati , Singh Lourembam Sanajaoba , Barman Mritunjoy , Dutta Subham , Tarafdar Jayanta 
Published: January 2023

Abstract
Tomato leaf curl New Delhi virus (ToLCNDV) is a whitefly-transmitted bipartite ssDNA virus causing worldwide havoc to the tomato production. The present study addresses first report for the existence of ToLCNDV at Manipur region of North East India. Sequence comparison of DNA A (MG649330) and DNA B (OM752176) genomes showed close homogeneity of about 98% and 100% with Bangladesh isolates, but revealed only 84–96% (DNA A) and 81–100% (DNA B) similarity with other isolates of ToLCNDV. Phylogenetic relationship of global ToLCV species showed interchangeable results, substantiating that the isolate belonged to the old world bipartite group. Significant recombination events were ascertained around the intergenic region and the 5’end of the AC1 gene implicating a constant driving force for evolution and emergence of the strain. Population dynamics of global ToLCNDV isolates and other dominant ToLCV species within India were determined based upon several parameters i.e. genetic diversity, historic demographic events including neutrality test, Fst distance, Mismatch distribution plot, Haplotype network yielding significant results. Population genetics analysis overall showed low nucleotide diversity indicating recent population expansion. The dominant species of ToLCV in India also reinforced similar results where all the population showed diversifying selection constraint for the coat protein genes. Population dynamics of these viruses portrays Indian subcontinent as the possible hotspot for rapid demographic expansion from a small virus population size, indicating probability for rapid spread and emergence of distinct strains which may assist in future to signal the emergence of new threats to tomato production.
Extracellular Matrix Based 3D Scaffold for Directing the Fate of Adult Stem Cells into Osteogenic Lineage
Authors: Ramzan Faiza , Khalid Shumaila , Salim Asmat , Frazier Trivia , Begum Sumreen , Mohiuddin Omair Anwar , Khan Irfan 
Published: January 2023

Abstract
Background
Bone defect causes softening of bone, reduction of bone density and mass, and degenerating bone microstructure which directly leads to bone fractures. Tissue-derived engineered cell-seeded hydrogel scaffolds are considered a promising strategy for the treatment of such defects. Among the most important objectives is to develop a unique biological tissue derived scaffolding material that is easily manipulated, efficient for cell attachment, and biocompatible, as well as supports cell growth, proliferation, and differentiation along osteogenic lineage.
Methods
This research describes a technique for the preparation of decellularized hydrogels. The detergent-based procedure using Triton-X-100 reported here effectively eliminates cellular proteins and antigens, as well as nucleic acid, while causing minimal damage to the extracellular matrix (ECM). The final product contains natural ECM components that guide stem cell differentiation by simulating the natural tissue microenvironment. Umbilical cord-derived mesenchymal stem cells (MSCs) were seeded in the hydrogel. The cell-hydrogel constructs were cultured in basal or osteogenic media for 28 days. Thereafter, mRNA and protein expression of osteogenic markers were analyzed.
Results
The results indicated that the scaffold had a fibrous structure with a relatively homogenous exterior. The cells seeded hydrogel after 28 days of culture in basal media as well as in osteogenic inductive media displayed higher mRNA and protein expression of osteogenic markers. Moreover, calcium deposits were also observed which further confirmed osteogenic differentiation.
Conclusion
The present findings demonstrate that the hydrogel is biocompatible with MSCs and possesses osteo-conductive and osteo-inductive capability in vitro. It is potentially useful for bone tissue engineering.
Do urban heat islands intensify droughts in tropical east Africa?
Authors: Garuma Gemechu Fanta 
Published: January 2023

Abstract
The horn of Africa is susceptible to droughts because of the persistent heat waves and insufficient precipitation. The growth of urban population and built-up urban environments exacerbate the overheating problems due to the urban heat island effects. Understanding the impacts of anthropogenic activities in such dry environments is important to control or mitigate extreme heat leading to droughts. This is required to preserve soil moisture, pothole waters, lakes and rivers that are required for pasture and drinking water. Nonetheless, the intensity and duration of the urban heat island effects have not been investigated in this region resulting in the underestimation of the intensity and severity of the extreme heat events. This study therefore performs the quantitative analyses of the intensity, duration and causality of the tropical surface urban heat islands (TSUHIs) for the first time using earth observation information at a regional to local scale. It also identifies the factors that control TSUHIs, considering background climate, population, vegetation and the impervious urban fractions. Results showed that the TSUHI in the capital cities of tropical east Africa varies from 1oC in Dodoma to 4oC in Kampala and reaches up to 8oC in Khartoum. The mean temperature contribution to regional climate from 2000-2020 is 0.64oC during the day and 0.34oC during the night, a mean total of around 0.5oC, a 0.25oC increase per decade. This is a quarter of the increase in global surface temperature, which is ~1.09oC from 2011 to 2020 compared to the 1850-1900 level. Most of these capital cities in this region exhibited high TSUHIs from late summer to winter and are dependent on mainly population, vegetation, evapotranspiration and soil moisture in different proportions.This urban induced additional temperature has been intensifying droughts in tropical east Africa. Therefore, urban planners are advised to consider the impacts of TSUHIs to reduce the severity of droughts in the tropical east Africa region.
Filling a Gap between Integrated Methods in the Digital Corpus Study of Literary Retranslations: The Case of To the Lighthouse into Italian
Authors: Cipriani Anna Maria 
Published: January 2023

Abstract
Digital technologies can be used to complement traditional descriptive translation studies (DTS) with various types of textual explorations. However, there is still a historical discrepancy between the digital corpus techniques applied to DTS drawing on corpus linguistics mainly focusing on linguistic features of translation and the DTS focusing on non-linguistic categories. Drawing on Zanettin’s (2011, 2013) proposed interpretative annotations, the methodology developed here extends the digital corpus approach to investigate whether and how the non-linguistic modernist features of Virginia Woolf’s To the Lighthouse are rendered in multiple (re)translations into Italian. This extension of the corpus approach fits with Bassnett’s and Lefevere’s (1990) ‘cultural turn’ from linguistic translation theories to Even-Zohar’s (1978/2012, 1979) and Toury’s (1980, 1981, 1995) polysystems theory for contextual (historical, social, and cultural) explanations, which are the defining basis of the DTS approach. In the case study considered here, the exhaustive and complete analysis demonstrates that the multiple retranslations are ‘modernist’ only marginally.
Multimodal Fusion Models for Pulmonary Embolism Mortality Prediction
Authors: Cahan Noa , Marom Edith M , Soffer Shelly , Barash Yiftach , Konen Eli , Klang Eyal , Greenspan Hayit 
Published: January 2023

Abstract
Pulmonary embolism (PE) is a common, life threatening cardiovascular emergency. Risk stratification is one of the core principles of acute PE management and determines the choice of diagnostic and therapeutic strategies. In routine clinical practice, clinicians rely on the patient’s electronic health record (EHR) to provide a context for their medical imaging interpretation. Most deep learning models for radiology applications only consider pixel-value information without the clinical context. Only a few integrate both clinical and imaging data. In this work, we develop and compare multimodal fusion models that can utilize weakly-labeled multimodal data by combining both volumetric pixel data and clinical patient data for automatic risk stratification of PE. Our best performing model is an intermediate fusion model that incorporates both bilinear attention and TabNet, and can be trained in an end-to-end manner. The results show that multimodality boosts performance by up to 14% with an area under the curve (AUC) of 0.96 for assessing PE severity, with a sensitivity of 90% and specificity of 94%, thus pointing to the value of using multimodal data to automatically assess PE severity.
Predictors of recurrent ischemic stroke: A retrospective cohort study
Authors: Shaafi Sheida , Sadeghpour Yalda , Masroor Fatemeh , Nejadghaderi Seyed Aria 
Published: January 2023

Abstract
Background: Cerebrovascular events are one of the most common causes of disability and the third leading cause of death in developed countries. Herein, we aimed to investigate the potential underlying causes of recurrent ischemic stroke.
Methods: This study was a retrospective cohort study which conducted in Razi Hospital, Tabriz, Iran between March 2018 and September 2020. Patients with the diagnosis of recurrent ischemic stroke during the study period were eligible to be included. Baseline characteristics and data on the recurrence were collected using chart reviews. Binary logistic regression was used to examine the factors related with recurrence.
Results: One hundred participants with a mean age of 68.88 years were included in the study among them 59% were male. Hypertension, diabetes, heart disease, smoking and hyperlipidemia identified in 79%, 42%, 24%, 15%, and 15%, respectively. The average recurrence time of stroke was about 2.52 years. There was only a significant relationship between history of heart diseases and ischemic stroke recurrence (risk ratio: 0.10: 95% confidence interval: 0.03-0.33).
Conclusions: We found no significant association between ischemic stroke recurrence and underlying diseases except for heart diseases. Further large-scale observational studies and meta-analysis are recommended to evaluate the association.