bluuebunny/crossref_metadata_2025_split huggingface dataset:
GNOMES II: Analysis of the Galactic diffuse molecular ISM in all four ground state hydroxyl transitions usingAmoeba
Authors: Hafner Anita , Dawson J. R. , Nguyen Hiep , Heiles Carl , Wardle M. , Lee M.-Y. , Murray Claire E. , Thompson K. L. , Stanimirović Snežana 
Published: February 2023

AbstractWe present observations of the four$^2 \Pi _{3/2}\,J=3/2$ground-rotational state transitions of the hydroxyl molecule (OH) along 107 lines of sight both in and out of the Galactic plane: 92 sets of observations from the Arecibo telescope and 15 sets of observations from the Australia Telescope Compact Array (ATCA). Our Arecibo observations included off-source pointings, allowing us to measure excitation temperature ($T_{\rm ex}$) and optical depth, while our ATCA observations give optical depth only. We perform Gaussian decomposition using the Automated Molecular Excitation Bayesian line-fitting Algorithm ‘Amoeba’ (Petzler, Dawson, & Wardle 2021, ApJ, 923, 261) fitting all four transitions simultaneously with shared centroid velocity and width. We identify 109 features across 38 sightlines (including 58 detections along 27 sightlines with excitation temperature measurements). While the main lines at 1665 and 1667 MHz tend to have similar excitation temperatures (median$|\Delta T_{\rm ex}({\rm main})|=0.6\,$K, 84% show$|\Delta T_{\rm ex}({\rm main})|<2\,$K), large differences in the 1612 and 1720 MHz satellite line excitation temperatures show that the gas is generally not in LTE. For a selection of sightlines, we compare our OH features to associated (on-sky and in velocity) Hi cold gas components (CNM) identified by Nguyen et al. (2019, ApJ, 880, 141) and find no strong correlations. We speculate that this may indicate an effective decoupling of the molecular gas from the CNM once it accumulates.
Honey bee larval culture in vitro: gut emptying determines the transition from larva to prepupa and recombinant AccApidaecin improves antibacterial activity
Authors: Chen Wen-Feng , Wang Hong-Fang , Wang Ying , Liu Zhen-Guo , Xu Bao-Hua 
Published: February 2023

AbstractIn vitro rearing of honey bee larvae is ideal for bioassay studies; no honey bee stable cell lines are available. Inconsistency of internal development staging of reared larvae and a susceptibility to contamination are common problems encountered. Standardized protocols on rearing larvae in vitro to make the larvae growth and development more similar to that of natural colonies are necessary to ensure the accuracy of experimental results and promote honey bee research as a model organism. Here, we concluded that when larval fasting weight was >160 mg, the time point of gut emptying can be defined as the critical point separating the larval and prepupal stages. In this way, we can conduct precise studies on the prepupal stage, such as organ remodeling during metamorphosis. Simultaneously, we further verified that recombinant AccApidaecin in genetic engineered bacteria added to the larval diet upregulated antibacterial peptide gene expression, and did not stimulate the stress response in larvae, nor did it affect the pupation rate or eclosion rate. This demonstrated that feeding recombinant AccApidaecin can enhance the individual antibacterial ability at the molecular level.
Definition of early age at onset in bipolar disorder according to distinctive neurodevelopmental pathways: insights from the FACE-BD study
Authors: Corponi Filippo , Lefrere Antoine , Leboyer Marion , Bellivier Frank , Godin Ophelia , Loftus Josephine , Courtet Philippe , Dubertret Caroline , Haffen Emmanuel , Llorca Pierre Michel , Roux Paul , Polosan Mircea , Schwan Raymund , Samalin Ludovic , Olié Emilie , Etain Bruno ,   FACE-BD (FondaMental Academic Centers of Expertise for Bipolar Disorder) Groups, Seriès Peggy , Belzeaux Raoul 
Published: February 2023

AbstractBackgroundConverging evidence suggests that a subgroup of bipolar disorder (BD) with an early age at onset (AAO) may develop from aberrant neurodevelopment. However, the definition of early AAO remains unprecise. We thus tested which age cut-off for early AAO best corresponds to distinguishable neurodevelopmental pathways.MethodsWe analyzed data from the FondaMental Advanced Center of Expertise-Bipolar Disorder cohort, a naturalistic sample of 4421 patients. First, a supervised learning framework was applied in binary classification experiments using neurodevelopmental history to predict early AAO, defined either with Gaussian mixture models (GMM) clustering or with each of the different cut-offs in the range 14 to 25 years. Second, an unsupervised learning approach was used to find clusters based on neurodevelopmental factors and to examine the overlap between such data-driven groups and definitions of early AAO used for supervised learning.ResultsA young cut-off, i.e. 14 up to 16 years, induced higher separability [mean nested cross-validation test AUROC = 0.7327 (± 0.0169) for ⩽16 years]. Predictive performance deteriorated increasing the cut-off or setting early AAO with GMM. Similarly, defining early AAO below 17 years was associated with a higher degree of overlap with data-driven clusters (Normalized Mutual Information = 0.41 for ⩽17 years) relatively to other definitions.ConclusionsEarly AAO best captures distinctive neurodevelopmental patterns when defined as ⩽17 years. GMM-based definition of early AAO falls short of mapping to highly distinguishable neurodevelopmental pathways. These results should be used to improve patients' stratification in future studies of BD pathophysiology and biomarkers.
Delayed sleep phase disorder during the COVID-19 pandemic and its health implications
Authors: Jiménez-Correa Ulises , Bonilla Naylea , Álvarez-García Horacio B. , Méndez-Alonzo Gerardo , Barrera-Medina Andrés , Santana-Miranda Rafael , Poblano Adrián , Marín-Agudelo Hernán A. 
Published: February 2023

AbstractCircadian rhythm sleep disorders are alterations that are characterized by a shift in the sleep-wake cycle relative to day and night, such as the delayed sleep phase disorder (DSPD), which is a retard of at least 2 hours in the sleep start. Typically, the patient falls asleep after 2 a.m. and wakes up after 10 a.m. and with symptom of sleep onset insomnia. The prevalence of DSPD in young adults is 0.48%, increasing to 3.3% in adolescents. Interestingly, patients with COVID-19 infection report anxiety due to the intensive care unit lockdown and constant exposure to bright light. In addition, post-COVID patients have an increased risk of developing DSPD. For example, in adolescent post-COVID patients, the prevalence of DSPD increases to 63.3%. Patients with DSPD also have alterations in metabolic health, poor school performance, cognitive impairment, and a higher risk of developing other diseases. The objective of the present review is therefore to describe the characteristics of DSPD during the COVID-19 pandemic and to outline its possible implications for physical health (eg, metabolism) and mental health (eg, anxiety or depression).
Development and genomic characterization of EMS induced mutant population of Zea mays L.
Authors: Kashif Syed Zain , Aslam Muhammad , Ahmed Zaheer , Saleem Fouzia , Alghabari Fahad , Alsamadany Hameed , Alzahrani Hind A. S. , Awan Faisal Saeed 
Published: February 2023

AbstractMaize is among major field crops which provides food, fodder and various byproducts to the industry. Development of better performing varieties is very important to enhance and strengthen the maize production system. In this study ethyl methanesulfonate (EMS) is used to induce genetic variation in maize. Mutant population was derived from two genotypes 100,003 and 100,004. EMS was applied under three different concentrations of 25, 50 and 75 mM. 25 mM was found as an ideal concentration resulting in maximum survival rate. Total 10 SSRs were used in this study, which amplified 28 alleles with average of 2.7 alleles. Analysis of molecular variance showed significant differences present among individuals. Average heterozygosity for mutants derived from 100,003 and 100,004 was 0.58 and 0.53, respectively. UPGMA analysis characterized the mutants into two main and many sub clusters. According to the principal component analysis, PC 1 and 2 contributed to 64.2% variability with eigenvalue greater than 1. Statistics showed maximum coefficients of variance in traits of leaf area, cobb height and plant height. Promising mutants were also identified and recommended for future breeding programme. In conclusion, EMS mutagenesis is an effective technique to develop novel mutants that can be exploited in future breeding programmes.
Huge Variation in H2 Generation During Seawater Alteration of Ultramafic Rocks
Authors: Ely T. D. , Leong J. M. , Canovas P. A. , Shock E. L. 
Published: February 2023

AbstractWater‐rock reactions liberate bioavailable energy, a necessary condition for chemotrophic habitability and origins of life. The major minerals of ultramafic (UM) rocks: olivine (ol), orthopyroxene (opx), and clinopyroxene (cpx), are of particular astrobiological interest as they are widespread in the solar system and known to produce significant quantities of H2 during aqueous alteration (serpentinization). H2 yields energy to life when oxidized by chemical species common to planetary fluids and is the most deeply rooted metabolite in Earth's phylogenetic tree. However, while field observations and calculations have corroborated the H2‐generating potential of UM rocks, specific formation pathways remain elusive, as the alteration assemblages in natural samples contain substantial heterogeneity. Here we show that variable UM compositions, temperature (T), and the mass ratio of Earth's seawater to rock (w/r) conspire to segregate alteration systems into those that produce supra‐mmol H2, and those that produce sub‐µmol H2. The oxidation of UM Fe(II) to Fe(III) provides the electrons necessary to reduce H2O to H2. However, the Fe(III)‐bearing phases that facilitate this process form together with more abundant phases competing for the same elements, including Ca, Si, and Fe(II). As a result, H2 abundance is determined by non‐redox‐active elements. Maximum H2 at high T requires low Si and is generally supported by Fe(III)‐serpentine and magnetite formation. Andradite formation, which stores Fe(III) but requires elevated Ca abundance, throttles up H2 production as T decreases in aging systems. These conditions are achieved in ol‐ and cpx‐rich rocks while opx‐rich rocks sequester less Fe(III), producing less H2.
Potential Impact of Winter–Spring North Atlantic Tripole SSTAs on the Following Autumn–Winter El Niño–Southern Oscillation: Bridging Role of the Tibetan Plateau
Authors: Yu Wei , Liu Yimin , Zhang Tuantuan , Yang Song , Wu Guoxiong , Chen Dake , Wang Ziqian , Yang Xiu‐Qun , Xu Lianlian , He Bian 
Published: February 2023

AbstractSea surface temperature anomalies (SSTAs) over the North Atlantic could stimulate the El Niño–Southern Oscillation (ENSO) events through regulating the tropical and mid‐latitude atmospheric circulations. Whether the Tibetan Plateau (TP) play an important bridging role in the mid‐latitude pathway has been rarely considered. Observational analysis and model simulations show that the spring TP surface wind speed dipole mode forced by the winter–spring North Atlantic tripole SSTAs can induce surface zonal wind anomalies over the equatorial western Pacific from April to June through the Indo‐Pacific gearing process, favoring the occurrence of subsequent autumn–winter ENSO events through the Bjerknes feedback. Moreover, a flattened TP will obviously weaken the atmospheric and oceanic responses associated with ENSO development to the North Atlantic tripole SSTAs forcing. Quantitatively, the TP's bridging effect accounts for about 38% proportion in the above process. Our finding provides a new insight in understanding the mid‐latitude pathway.
Spectral Variability of Rocks and Soils on the Jezero Crater Floor: A Summary of Multispectral Observations From Perseverance's Mastcam‐Z Instrument
Authors: Rice M. S. , Johnson J. R. , Million C. C. , St. Clair M. , Horgan B. N. , Vaughan A. , Núñez J. I. , Garczynski B. , Curtis S. , Kinch K. B. , Merusi M. , Hayes A. , Bell J. F. , Duflot L. , Lapo K. , Evans A. A. , Eng A. , Cloutis E. , Brown A. , Annex A. M. 
Published: February 2023

AbstractNASA's Mars‐2020 Perseverance rover spent its first year in Jezero crater studying the mafic lava flows of the Máaz formation and the ultramafic cumulates of the Séítah formation, both of which have undergone minor alteration and are variably covered by coatings, dust, and/or soil deposits. Documenting the rock and soil characteristics across the crater floor is critical for establishing the geologic context of Perseverance's cached samples—which will eventually be returned to Earth—and for interpreting the deposition and modification of the Máaz and Séítah formations. Mastcam‐Z, a pair of multispectral, stereoscopic zoom‐lens cameras, provides broadband red/green/blue and narrowband visible to near‐infrared images (VNIR, 440–1,020 nm). From multispectral observations from sols 0 to 380, we compiled a database of ∼2,400 representative Mastcam‐Z spectra. We analyzed principal components, spectral parameters, and laboratory spectra of pure minerals and natural rock surfaces to interpret the spectral diversity of rocks and soils. We define eight spectral classes of rocks: Dusty, Hematite‐like, Coated, Low‐Ca Pyroxene‐like, Olivine‐like, Weathered Olivine‐like, Fe‐rich Pyroxene‐like, and Dark Oxide‐like. The variability of soil spectra in the Jezero crater floor is controlled primarily by the amount of dust and indicates a largely consistent soil mineralogy across the traverse, with the exception of the area disturbed by the landing event. In comparison to rock spectra from the Curiosity rover's Mastcam instrument in Gale crater, rocks on the Jezero crater floor are generally less spectrally diverse, but the Olivine‐like rocks within the Séítah formation represent a new spectral rock class in Mars surface exploration.
Long non-coding RNA DLX6-AS1 is the key mediator of glomerular podocyte injury and albuminuria in diabetic nephropathy by targeting the miR-346/GSK-3β signaling pathway
Authors: Guo Jia , Zheng Wen , Liu Yong , Zhou Mengwen , Shi Yan , Lei Min , Zhang Chaojie , Liu Zhangsuo 
Published: February 2023

AbstractProgressive albuminuria is the primary clinical symptom of diabetic nephropathy (DN), leading to a gradual decline in kidney function. DLX6-AS1 was the first reported long non-coding RNA (lncRNA) to participate in organogenesis and play crucial roles in the brain or neural cell development. Herein, we investigated the DLX6-AS1 (Dlx6-os1 in mice) role in DN pathogenesis. We found that DLX6-AS1 expression in DN patients correlated with the extent of albuminuria. Dlx6-os1 overexpression induced cellular damage and inflammatory responses in cultured podocytes through miR-346-mediated regulation of the GSK-3β pathway. In various established diabetic and newly developed knockout mouse models, Dlx6-os1 knockdown/knockout significantly reduced podocyte injury and albuminuria. The Dlx6-os1 effects were remarkably modulated by miR-346 mimics or mutants and significantly diminished in podocyte-specific GSK-3β-knockout mice. Thus, DLX6-AS1 (Dlx6-os1) promotes DN development by accelerating podocyte injury and inflammation through the upregulation of the GSK-3β pathway, providing a novel molecular target for DN therapy.
The activation mechanism and antibody binding mode for orphan GPR20
Authors: Lin Xi , Jiang Shan , Wu Yiran , Wei Xiaohu , Han Gye-Won , Wu Lijie , Liu Junlin , Chen Bo , Zhang Zhibin , Zhao Suwen , Cherezov Vadim , Xu Fei 
Published: February 2023

AbstractGPR20 is a class-A orphan G protein-coupled receptor (GPCR) and a potential therapeutic target for gastrointestinal stromal tumors (GIST) owing to its differentially high expression. An antibody-drug conjugate (ADC) containing a GPR20-binding antibody (Ab046) was recently developed in clinical trials for GIST treatment. GPR20 constitutively activates Gi proteins in the absence of any known ligand, but it remains obscure how this high basal activity is achieved. Here we report three cryo-EM structures of human GPR20 complexes including Gi-coupled GPR20 in the absence or presence of the Fab fragment of Ab046 and Gi-free GPR20. Remarkably, the structures demonstrate a uniquely folded N-terminal helix capping onto the transmembrane domain and our mutagenesis study suggests a key role of this cap region in stimulating the basal activity of GPR20. We also uncover the molecular interactions between GPR20 and Ab046, which may enable the design of tool antibodies with enhanced affinity or new functionality for GPR20. Furthermore, we report the orthosteric pocket occupied by an unassigned density which might be essential for exploring opportunities for deorphanization.
Molecular exploration of fossil eggshell uncovers hidden lineage of giant extinct bird
Authors: Grealy Alicia , Miller Gifford H. , Phillips Matthew J. , Clarke Simon J. , Fogel Marilyn , Patalwala Diana , Rigby Paul , Hubbard Alysia , Demarchi Beatrice , Collins Matthew , Mackie Meaghan , Sakalauskaite Jorune , Stiller Josefin , Clarke Julia A. , Legendre Lucas J. , Douglass Kristina , Hansford James , Haile James , Bunce Michael 
Published: February 2023

AbstractThe systematics of Madagascar’s extinct elephant birds remains controversial due to large gaps in the fossil record and poor biomolecular preservation of skeletal specimens. Here, a molecular analysis of 1000-year-old fossil eggshells provides the first description of elephant bird phylogeography and offers insight into the ecology and evolution of these flightless giants. Mitochondrial genomes from across Madagascar reveal genetic variation that is correlated with eggshell morphology, stable isotope composition, and geographic distribution. The elephant bird crown is dated to ca. 30 Mya, when Madagascar is estimated to have become less arid as it moved northward. High levels of between-clade genetic variation support reclassifying Mullerornis into a separate family. Low levels of within-clade genetic variation suggest there were only two elephant bird genera existing in southern Madagascar during the Holocene. However, we find an eggshell collection from Madagascar’s far north that represents a unique lineage of Aepyornis. Furthermore, divergence within Aepyornis coincides with the aridification of Madagascar during the early Pleistocene ca. 1.5 Ma, and is consistent with the fragmentation of populations in the highlands driving diversification and the evolution of extreme gigantism over shorts timescales. We advocate for a revision of their taxonomy that integrates palaeogenomic and palaeoecological perspectives.
Structural mechanism for inhibition of PP2A-B56α and oncogenicity by CIP2A
Authors: Pavic Karolina , Gupta Nikhil , Omella Judit Domènech , Derua Rita , Aakula Anna , Huhtaniemi Riikka , Määttä Juha A. , Höfflin Nico , Okkeri Juha , Wang Zhizhi , Kauko Otto , Varjus Roosa , Honkanen Henrik , Abankwa Daniel , Köhn Maja , Hytönen Vesa P. , Xu Wenqing , Nilsson Jakob , Page Rebecca , Janssens Veerle , Leitner Alexander , Westermarck Jukka 
Published: February 2023

AbstractThe protein phosphatase 2A (PP2A) heterotrimer PP2A-B56α is a human tumour suppressor. However, the molecular mechanisms inhibiting PP2A-B56α in cancer are poorly understood. Here, we report molecular level details and structural mechanisms of PP2A-B56α inhibition by an oncoprotein CIP2A. Upon direct binding to PP2A-B56α trimer, CIP2A displaces the PP2A-A subunit and thereby hijacks both the B56α, and the catalytic PP2Ac subunit to form a CIP2A-B56α-PP2Ac pseudotrimer. Further, CIP2A competes with B56α substrate binding by blocking the LxxIxE-motif substrate binding pocket on B56α. Relevant to oncogenic activity of CIP2A across human cancers, the N-terminal head domain-mediated interaction with B56α stabilizes CIP2A protein. Functionally, CRISPR/Cas9-mediated single amino acid mutagenesis of the head domain blunted MYC expression and MEK phosphorylation, and abrogated triple-negative breast cancer in vivo tumour growth. Collectively, we discover a unique multi-step hijack and mute protein complex regulation mechanism resulting in tumour suppressor PP2A-B56α inhibition. Further, the results unfold a structural determinant for the oncogenic activity of CIP2A, potentially facilitating therapeutic modulation of CIP2A in cancer and other diseases.
Designing covalent organic frameworks with Co-O4 atomic sites for efficient CO2 photoreduction
Authors: Zhang Qian , Gao Shuaiqi , Guo Yingying , Wang Huiyong , Wei Jishi , Su Xiaofang , Zhang Hucheng , Liu Zhimin , Wang Jianji 
Published: February 2023

AbstractCobalt coordinated covalent organic frameworks have attracted increasing interest in the field of CO2 photoreduction to CO, owing to their high electron affinity and predesigned structures. However, achieving high conversion efficiency is challenging since most Co related coordination environments facilitate fast recombination of photogenerated electron-hole pairs. Here, we design two kinds of Co-COF catalysts with oxygen coordinated Co atoms and find that after tuning of coordination environment, the reported Co framework catalyst with Co-O4 sites exhibits a high CO production rate of 18000 µmol g−1 h−1 with selectivity as high as 95.7% under visible light irradiation. From in/ex-situ spectral characterizations and theoretical calculations, it is revealed that the predesigned Co-O4 sites significantly facilitate the carrier migration in framework matrixes and inhibit the recombination of photogenerated electron-hole pairs in the photocatalytic process. This work opens a way for the design of high-performance catalysts for CO2 photoreduction.
Rapid diagnosis of membranous nephropathy based on serum and urine Raman spectroscopy combined with deep learning methods
Authors: Zhang Xueqin , Song Xue , Li Wenjing , Chen Cheng , Wusiman Miriban , Zhang Li , Zhang Jiahui , Lu Jinyu , Lu Chen , Lv Xiaoyi 
Published: February 2023

AbstractMembranous nephropathy is the main cause of nephrotic syndrome, which has an insidious onset and may progress to end-stage renal disease with a high mortality rate, such as renal failure and uremia. At present, the diagnosis of membranous nephropathy mainly relies on the clinical manifestations of patients and pathological examination of kidney biopsy, which are expensive, time-consuming, and have certain chance and other disadvantages. Therefore, there is an urgent need to find a rapid, accurate and non-invasive diagnostic technique for the diagnosis of membranous nephropathy. In this study, Raman spectra of serum and urine were combined with deep learning methods to diagnose membranous nephropathy. After baseline correction and smoothing of the data, Gaussian white noise of different decibels was added to the training set for data amplification, and the amplified data were imported into ResNet, AlexNet and GoogleNet models to obtain the evaluation results of the models for membranous nephropathy. The experimental results showed that the three deep learning models achieved an accuracy of 1 for the classification of serum data of patients with membranous nephropathy and control group, and the discrimination of urine data was above 0.85, among which AlexNet was the best classification model for both samples. The above experimental results illustrate the great potential of serum- and urine-based Raman spectroscopy combined with deep learning methods for rapid and accurate identification of patients with membranous nephropathy.