bluuebunny/crossref_metadata_2025_split huggingface dataset:
Improved diagnosis of reflux hypersensitivity
Authors: Voulgaris Theodoros , Hoshino Shintaro , Sifrim Daniel , Yazaki Etsuro 
Published: September 2023

AbstractBackgroundReflux hypersensitivity (RH) is characterized by normal esophageal exposure to acid and positive correlation of symptoms to reflux episodes. Positivity of Symptomatic Index (SI) and/or Symptom Association Probability (SAP) is used diagnostically, though experts support that concordance of both is needed. We evaluated differences among patients with RH and concordance of SI/SAP or not.MethodsPatients with typical reflux symptoms without previous GERD diagnosis, submitted simultaneously to Ph‐Impedance off PPI and high resolution manometry were included. Self‐response to PPI was evaluated. Patients showing SI and/or SAP positivity were considered having RH and further classified to definite RH if both SI/SAP were positive or indefinite if only one positive.Key ResultsTotally 2659 patients (M/F: 35.6%/64.7%, mean age: 45 ± 14) were included. Final diagnosis was; FH: 21.8%, RH: 29.3% (definite: 14.3%/indefinite: 15%), GERD: 36% and inconclusive GERD: 12.9%. Patients with definite RH showed increased total reflux time, total number of reflux episodes and length of hiatus hernia, and also numerically but not statistically significant increased rates of PPI responsiveness versus indefinite RH and decreased mean nocturnal baseline impedance. Moreover, they showed significantly increased rate of PPI response versus patients with functional heartburn (FH).Conclusion & InferencesSome PPI responsiveness is frequent among patients with RH as also with FH, and cannot discriminate those entities clinically when diagnosing RH using SI and/or SAP positive criterion. Patients with RH and SI/SAP concordance differ from patients without. Implementation of a strict RH definition (both SI and SAP positive) can better distinguish RH from FH and should be used in the future.
Is it true that all human beings have dignity?
Authors: Ferdynus Marcin Paweł 
Published: September 2023

AbstractThe discussion around dignity in nursing philosophy has been underway for many years. The literature still lacks philosophical arguments that would justify the thesis that all people have dignity. Scholars who defend dignity as an intrinsic value most often refer to Kant. However, Kant does not seem to be the most suitable candidate to defend the thesis that all human beings possess dignity. In this paper, I attempt to show that Aristotle's and Aquinas's views can help justify this thesis. To this end, I distinguish between actual dignity, potential dignity, and existential dignity. I state that all human beings have existential dignity or potential dignity.
Randomised controlled trial of Jiandu granule in preventing chemoradiotherapy‐induced oral mucositis
Authors: Li Huakang , Ke Jianlong , Wang Xiaoyan , Xu Bo , Li Qiang , Wu Ziliang , Wang Yue , Lin Bing 
Published: September 2023

AbstractObjectiveThis clinical trial aimed to evaluate the efficacy and safety of Jiandu granule, a Chinese herbal medicine formula, for preventing oral mucositis in patients with nasopharyngeal carcinoma undergoing concurrent chemoradiotherapy.Materials and MethodsA single‐centre, open‐label, randomised controlled trial was conducted, enrolling 138 patients with locally advanced nasopharyngeal carcinoma. Patients were randomly allocated to either the experimental group (n = 69) or the control group (n = 69). Both groups received concurrent chemoradiotherapy and standard care for oral mucositis, with the experimental group additionally receiving Jiandu granule intervention. The primary outcome was the incidence of severe oral mucositis (grade III to IV).ResultsIn the full‐analysis set, severe oral mucositis occurred in 14 of 69 (20.3%) patients in the experimental group and 31 of 69 (44.9%) patients in the control group (absolute risk reduction: 24.6%; relative risk reduction: 54.8%; p = 0.002; number needed to treat: 4). Jiandu granule was associated with mild/moderate gastrointestinal reactions, with an overall incidence of 5.9%.ConclusionJiandu granule reduced the incidence of severe oral mucositis in nasopharyngeal carcinoma patients during concurrent chemoradiotherapy and had an acceptable safety profile.
Continued challenges in pediatric anesthesia during COVID‐19 in 2022: An international survey from the pediatric anesthesia COVID‐19 collaborative
Authors: Zhong John , Bradford Victoria , Fernandez Allison M. , Infosino Andrew , Soneru Codruta N. , Staffa Steven J. , Raman Vidya T. , Cravero Joseph , Zurakowski David , Meier Petra M. ,   The Pediatric Anesthesia COVID‐19 Collaborative
Published: September 2023

AbstractIntroductionThis international survey explored the ongoing impact of COVID‐19 on pediatric anesthesiology. It assessed COVID‐19's impact on the practice of pediatric anesthesiology, staffing, job satisfaction, and retention at the beginning of 2022 and addressed what should be done to ameliorate COVID‐19's impact and what initiatives hospitals had implemented.MethodsThis survey focused on five major domains: equipment/medication, vaccination/testing, staffing, burnout, and economic repercussions. Pilot testing for questionnaire clarity was conducted by members of the Pediatric Anesthesia COVID‐19 Collaborative. The survey was administered by e‐mail to a representative of the 72 collaborative centers. Respondents were instructed to answer based on their institution's practice from February through April of 2022. Descriptive statistics with 95% confidence intervals are reported.ResultsSeventy of seventy‐two institutions participated in this survey (97% response rate). Fifty‐nine (84%) were from the United States, and 11 (16%) included other countries. The majority experienced equipment (68%) and medication (60%) shortages. Many institutions reported staffing shortages in nursing (37%), perioperative staff (27%), and attending anesthesiologists (11%). Sixty‐two institutions (89%) indicated burnout was a frequent topic of conversation among pediatric anesthesiologists. Forty‐three institutions (61%) reported anesthesiologists leaving current practice and 37 (53%) early retirement. Twenty‐eight institutions (40%) canceled elective cases. The major suggestions for improving job retention included improving financial compensation (76%), decreasing clinical time (67%), and increasing flexibility in scheduled clinical time (66%). Only a minority of institutions had implemented the following initiatives: improving financial compensation (19%), increased access to mental health/counseling services (30%), and assistance with child or elder care (7%). At the time of the survey, 34% of institutions had not made any changes.ConclusionOur study found that COVID‐19 has continued to impact pediatric anesthesiology. There are major discrepancies between what anesthesiologists believe are important for job satisfaction and faculty retention compared to implemented initiatives. Data from this survey provide insight for institutions and departments for addressing these challenges.
Alleviation of ultraviolet‐B radiation‐induced photoaging using Saussurea medusa Maxim polysaccharide
Authors: Guo Yan , Kong Yue , Sun Juan , Jiao Yang , Hong Yan , Wang Yan 
Published: September 2023

AbstractSaussurea medusa polysaccharide, the polysaccharide extract of Saussurea medusa Maxim, a traditional Chinese herbal medicine, is used to combat intense ultraviolet radiation, cold, and hypoxia in patients, as well as during drought. This polysaccharide has rich medicinal and ecological values. We aimed to determine whether saussurea medusa polysaccharides can reduce ultraviolet B (UVB)‐induced skin photoaging. Seventy‐five male Kunming mice were divided into five groups: control, UVB‐only, UVB plus vitamin E (VE group), UVB plus saussurea medusa (2 g/kg), and UVB plus saussurea medusa (6 g/kg). The control group was irradiated with normal light, while the other four groups were subcutaneously administered 10 mL/kg/day D‐galactose and irradiated with narrow‐spectrum UVB for 40 min daily. From day 11, the VE group was administered 0.25 g/kg/day vitamin E, while the saussurea medusa intervention groups were administered 2 and 6 g/kg/day saussurea medusa polysaccharide. After 30 days of continuous administration, treatment with saussurea medusa polysaccharides was found to reduce UVB‐induced skin photoaging in mice by elevating the levels of superoxide dismutase, glutathione peroxidase, and hydroxyproline (HYP), while reducing the level of MDA, and inhibiting the EGFR/MEK/ERK/c‐Fos pathway. Overall, our findings suggest that treatment with saussurea medusa polysaccharides positively influences skin photoaging.
Restricted O2 consumption in pea roots induced by hexanoic acid is linked to depletion of Krebs cycle substrates
Authors: Casolo Valentino , Zancani Marco , Pellegrini Elisa , Filippi Antonio , Gargiulo Sara , Konnerup Dennis , Morandini Piero , Pedersen Ole 
Published: September 2023

AbstractPlant roots are exposed to hypoxia in waterlogged soils, and they are further challenged by specific phytotoxins produced by microorganisms in such conditions. One such toxin is hexanoic acid (HxA), which, at toxic levels, causes a strong decline in root O2 consumption. However, the mechanism underlying this process is still unknown. We treated pea (Pisum sativum L.) roots with 20 mM HxA at pH 5.0 and 6.0 for a short time (1 h) and measured leakage of key electrolytes such as metal cations, malate, citrate and nonstructural carbohydrates (NSC). After treatment, mitochondria were isolated to assess their functionality evaluated as electrical potential and O2 consumption rate. HxA treatment resulted in root tissue extrusion of K+, malate, citrate and NSC, but only the leakage of the organic acids and NSC increased at pH 5.0, concomitantly with the inhibition of O2 consumption. The activity of mitochondria isolated from treated roots was almost unaffected, showing just a slight decrease in oxygen consumption after treatment at pH 5.0. Similar results were obtained by treating the pea roots with another organic acid with a short carbon chain, that is, butyric acid. Based on these results, we propose a model in which HxA, in its undissociated form prevalent at acidic pH, stimulates the efflux of citrate, malate and NSC, which would, in turn, cause starvation of mitochondrial respiratory substrates of the Krebs cycle and a consequent decline in O2 consumption. Cation extrusion would be a compensatory mechanism in order to restore plasma membrane potential.
Oscillations in three‐reaction quadratic mass‐action systems
Authors: Banaji Murad , Boros Balázs , Hofbauer Josef 
Published: September 2023

AbstractIt is known that rank‐two bimolecular mass‐action systems do not admit limit cycles. With a view to understanding which small mass‐action systems admit oscillation, in this paper we study rank‐two networks with bimolecular source complexes but allow target complexes with higher molecularities. As our goal is to find oscillatory networks of minimal size, we focus on networks with three reactions, the minimum number that is required for oscillation. However, some of our intermediate results are valid in greater generality.One key finding is that an isolated periodic orbit cannot occur in a three‐reaction, trimolecular, mass‐action system with bimolecular sources. In fact, we characterize all networks in this class that admit a periodic orbit; in every case, all nearby orbits are periodic too. Apart from the well‐known Lotka and Ivanova reactions, we identify another network in this class that admits a center. This new network exhibits a vertical Andronov–Hopf bifurcation.Furthermore, we characterize all two‐species, three‐reaction, bimolecular‐sourced networks that admit an Andronov–Hopf bifurcation with mass‐action kinetics. These include two families of networks that admit a supercritical Andronov–Hopf bifurcation and hence a stable limit cycle. These networks necessarily have a target complex with a molecularity of at least four, and it turns out that there are exactly four such networks that are tetramolecular.
Brain activation associated with low‐ and high‐intensity concentric versus eccentric isokinetic contractions of the biceps brachii: An fNIRS study
Authors: Teo Wei‐Peng , Tan Clara Xinru , Goodwill Alicia M. , Mohammad Saqif , Ang Yi‐Xuan , Latella Christopher 
Published: September 2023

AbstractStudies have shown that neural responses following concentric (CON) and eccentric (ECC) muscle contractions are different, which suggests differences in motor control associated with CON and ECC contractions. This study aims to determine brain activation of the left primary motor cortex (M1) and left and right dorsolateral prefrontal cortices (DLPFCs) during ECC and CON of the right bicep brachii (BB) muscle at low‐ and high‐contraction intensities. Eighteen young adults (13M/5F, 21–35 years) were recruited to participate in one familiarization and two testing sessions in a randomized crossover design. During each testing session, participants performed either ECC or CON contractions of the BB (3 sets × 8 reps) at low‐ (25% of maximum ECC/CON, 45°/s) and high‐intensity (75% of maximum ECC/CON, 45°/s) on an isokinetic dynamometer. Eleven‐channel functional near‐infrared spectroscopy was used to measure changes in oxyhemoglobin (O2Hb) from the left M1, and left and right DLPFC during ECC and CON contractions. Maximum torque for ECC was higher than CON (43.3 ± 14.1 vs. 46.2 ± 15.7 N m, p = 0.025); however, no differences in O2Hb were observed between contraction types at low or high intensities in measured brain regions. High‐intensity ECC and CON contractions resulted in greater increases in O2Hb of M1 and bilateral DLPFC compared to low‐intensity ECC and CON contractions (p = 0.014). Our findings suggest no differences in O2Hb responses between contraction types at high and low intensities. High‐contraction intensities resulted in greater brain activation of the M1 and bilateral DLPFC, which may have implications for neurorehabilitation to increase central adaptations from exercise.
Are software automation and teleworker substitutes? Preliminary evidence from Japan
Authors: Baldwin Richard , Okubo Toshihiro 
Published: September 2023

AbstractDigital technology is reshaping workplaces by enabling spatial separation of offices, known as telework, or remote intelligence (RI) and by facilitating automation of service sector tasks via artificial intelligence (AI). This paper is the first attempt to empirically investigate whether AI and RI are complements or substitutes in the service sector. It uses a worker‐level panel of surveys collected from around 10,000 workers from pre‐COVID‐19 pandemic to late 2022. We find preliminary evidence that suggests that AI and RI are complements rather than substitutes. The evidence comes first from the positive correlation of investments in AI‐promoting and RI‐promoting software at the firm and worker level, and second from the positive correlation of workers' expectations regarding telework and software automation. The evidence is far from definitive but suggests that the complement‐substitution question is a fruitful line for future research.
Exercise breaks prevent attenuation in cerebrovascular function following an acute bout of uninterrupted sitting in healthy children
Authors: Tallon Christine M. , Nowak‐Flück Daniela , Reiger Mathew G. , Green Daniel J. , Tremblay Mark S. , Ainslie Phil N. , McManus Ali M. 
Published: September 2023

AbstractThe purpose of this study was to examine the effect of an acute bout of prolonged sitting with and without exercise breaks on cerebrovascular function in 7‐ to 13‐year‐old children. Forty‐two children and adolescents were recruited to a crossover trial, with 15 girls (mean age 10.1 ± 2.5 years) and 16 boys (mean age 10.5 ± 1.3 years) completing the two trial conditions: SIT, uninterrupted sitting for 3 h and CYCLE, 3 h of sitting interrupted hourly with a 10‐min moderate intensity exercise break. Cerebrovascular function was measured Pre and Post SIT and CYCLE from blood flow (), diameter, and shear rate of the internal carotid artery (ICA) at rest and in response to CO2. Blood velocity in the middle (MCA) and posterior (PCA) cerebral arteries was assessed at rest, during a neurovascular coupling task (NVC) and in response to CO2. We demonstrate that SIT but not CYCLE reduced ICA cerebrovascular reactivity to CO2 (%Δ ICA /Δ end‐tidal CO2: SIT: Pre 5.0 ± 2.4%/mmHg to Post 3.3 ± 2.8%/mmHg vs. CYCLE: Pre 4.4 ± 2.3%/mmHg to Post 5.3 ± 3.4%/mmHg, P = 0.05) and slowed the MCA blood velocity onset response time to hypercapnia (SIT: Pre 57.2 ± 32.6 s to Post 76.6 ± 55.2 s, vs. CYCLE: Pre 64.1 ± 40.4 s to Post 52.3 ± 28.8 s, P = 0.05). There were no changes in NVC. Importantly, breaking up prolonged sitting with hourly exercise breaks prevented the reductions in cerebrovascular reactivity to CO2 and the slowed intracranial blood velocity onset response time to hypercapnia apparent with uninterrupted sitting in children.
Temporal optimization of exercise to lower fasting glucose levels
Authors: Kanaley Jill A. , Porter J. W. , Winn N. C. , Lastra G. , Chockalingam A. , Pettit‐Mee R. J. , Petroski G. F. , Cobelli C. , Schiavon M. , Parks E. J. 
Published: September 2023

AbstractExercise stimulates glucose uptake and increases insulin sensitivity acutely. Temporally optimizing exercise timing may minimize the nocturnal rise in glucose levels. This study examined the effect of exercise timing on evening and overnight glucose concentrations in individuals who were non‐obese with normal fasting glucose levels (Non‐Ob; n = 18) and individuals with obesity (OB) with impaired fasting glucose levels (OB+IFG) and without (n = 16 and n = 18, respectively). Subjects were studied on three occasions (no exercise (NOEX)), morning exercise (AMEX; 0700 h) and evening exercise (PMEX; 2000 h). The evening meal was provided (1800 h) and blood samples were taken from 1740 to 0700 h and morning endogenous glucose production (EGP) was measured. Glucose and insulin concentrations increased with the dinner meal with peak concentrations being higher in OB+IFG than in OB and Non‐Ob (P = 0.04). In OB+IFG, evening glucose concentrations rose above baseline levels at about 2300 h, with the glucose concentrations staying somewhat lower with AMEX and PMEX until ∼0500 h than with NOEX. In OB+IFG, insulin concentrations decreased following the dinner meal and waned throughout the night, despite the rising glucose concentrations. In the OB and Non‐Ob individuals following the dinner meal, no increase in glucose concentrations occurred in the evening period and insulin levels mirrored this. No difference was observed in the morning fasting glucose levels between study days or between groups. Regardless of time of day, exercise delays the evening rise in glucose concentrations in adults with OB+IFG but does not lower morning fasting glucose levels or improve the synchrony between glucose and insulin concentrations.
imageKey points
Insulin resistance and type 2 diabetes have been linked to disturbances of the core clock, and glucose tolerance demonstrates a diurnal rhythm in healthy humans with better glucose tolerance in the morning than in the afternoon and evening.
Skeletal muscle is a primary site for insulin resistance in people with impaired glucose tolerance.
In individuals with obesity and impaired fasting glucose levels (OB+IFG), following a dinner meal, glucose concentrations started to rise and continues throughout the night, resulting in elevated glucose levels, while concomitantly, insulin levels are waning.
Exercise, regardless of the time of day, suppressed the rise in glucose levels in OB+IFG for many hours during the night but did not lower morning fasting glucose levels. Morning exercise was not quite as effective as evening exercise.
Acetylation of K188 and K192 inhibits the DNA-binding ability of NarL to regulate
Salmonella
virulence
Authors: Zhang Liu-Qing , Shen Yi-Lin , Ye Bang-Ce , Zhou Ying 
Published: September 2023

ABSTRACT

Salmonella
infection significantly increases nitrate levels in the intestine, immune cells, and immune organs of the host, and it can exploit nitrate as an electron acceptor to enhance its growth. In the presence of nitrate or nitrite, NarL, a regulatory protein of the Nar two-component system, is activated and regulates a number of genes involved in nitrate metabolism. However, research on NarL at the post-translational level is limited. In this study, we demonstrate that the DNA-binding sites K188 and 192 of NarL can be acetylated by bacterial metabolite acetyl phosphate and that the degree of acetylation has a considerable influence on the regulatory function of NarL. Specifically, acetylation of NarL negatively regulates the transcription of
narG
,
narK,
and
napF
, which affects the utilization of nitrate in
Salmonella
. Besides, both cell and mouse models show that acetylated K188 and K192 result in attenuated replication in RAW 264.7 cells, as well as impaired virulence in mouse model. Together, this research identifies a novel NarL acetylation mechanism that regulates
Salmonella
virulence, providing a new insight and target for salmonellosis treatment.

IMPORTANCE

Salmonella
is an important intracellular pathogen that can cause limited gastroenteritis and self-limiting gastroenteritis in immunocompetent humans. Nitrate, the highest oxidation state form of nitrogen, is critical in the formation of systemic infection in
Salmonella
. It functions as a signaling molecule that influences
Salmonella
chemotaxis, in addition to acting as a reduced external electron acceptor for
Salmonella
anaerobic respiration. NarL is an essential regulatory protein involved in nitrate metabolism in
Salmonella
, and comprehending its regulatory mechanism is necessary. Previous research has linked NarL phosphorylation to the formation of its dimer, which is required for NarL to perform its regulatory functions. Our research demonstrated that acetylation also affects the regulatory function of NarL. We found that acetylation affects
Salmonella
pathogenicity by weakening the ability of NarL to bind to the target sequence, further refining the mechanism of the anaerobic nitrate respiration pathway.
A coating of lipoproteins provides a stabilizing environment on the inner membrane of
Bacillus subtilis
spores
Authors: Moir Anne , Christie Graham 
Published: September 2023

ABSTRACT

A new study by M. J. Flores, K. Duricy, S. Choudhary, M. Laue, and D. L. Popham (J Bacteriol 205:e00142-23, 2023,
https://doi.org/10.1128/jb.00142-23
) demonstrates a role for the YlaJ/YhcN family of lipoproteins in the immobilization of the spore’s inner membrane. In the absence of these lipoproteins, membrane fluidity increases and membrane-associated proteins like the GerA receptor complexes are more exposed to inimical conditions. The role of these proteins in stabilizing the
Bacillus
spore inner membrane is now being explored.