bluuebunny/crossref_metadata_2025_split huggingface dataset:
Genomic and functional impact of Trp53 inactivation in JAK2V617F myeloproliferative neoplasms
Authors: GIRAUDIER Stéphane , Gou Panhong , Liu Duanya , Ganesan Saravanan , Lauret Evelyne , Maslah Nabih , Zhang Wenchao , Parietti Veronique , Meignin Véronique , Kiladjian Jean-Jacques , Cassinat Bruno 
Published: July 2023

Abstract

Classical myeloproliferative neoplasms (MPNs) are characterized by the proliferation of myeloid cells and the risk of transformation into myelofibrosis or acute myeloid leukemia (AML) and TP53 mutations in MPN patients are linked to AML. However, JAK2V617F has been reported to impact the TP53 response to DNA damage, suggesting potential overlapping role of TP53 inactivation in MPN. We established a mouse model showing that JAK2V617F/Vav-Cre/Trp53−/− mice displayed a similar phenotype to JAK2V617F/Vav-Cre mice, but their proliferation was outcompeted in competitive grafts. RNA-Seq revealed that half of the genes affected by JAK2V617F were p53-dependent, including the interferon pathway. To validate this finding, mice were repopulated with a mixture of wild-type and JAK2V617F (or JAK2V617F/Vav-Cre/Trp53−/−) cells and treated with pegylated interferonα. JAK2V617F-reconstituted mice entered complete hematological remission, while JAK2V617F/Vav-Cre /Trp53−/−-reconstituted mice did not, confirming that p53 loss induced interferon-α resistance. KEGG and Gene Ontology analyses of common deregulated genes showed that these genes were mainly implicated in cytokine response, proliferation, and leukemia evolution, illustrating that in this mouse model, the development of MPN is mainly Trp53-independent. Taken together, our results show that many genetic modifications induced by JAK2V617F are p53-dependent, the MPN phenotype may not be. Trp53 loss alone is insufficient to induce rapid leukemic transformation in steady-state hematopoiesis in JAK2V617F MPN, and Trp53 loss may contribute to interferon resistance in MPN.
Discovery of a novel low-cost medium-entropy stainless steel with exceptional mechanical behavior over a wide temperature range
Authors: Shen Tong , Wen Kangkang , Cai Xuecheng , Sun B. , Feng Rui , Du Congcong , Zhu Huihui , An Ke , Wu Yuan , Ding Shuaijun , Meng Fanxi , Xin S. , Bai Zhenhua , Liaw Peter 
Published: July 2023

Abstract
Over 100 years, stainless steels have been extensively used as engineering materials in many areas1-4. However, the strength-ductility trade-off1,3 and insufficient elevated-temperature strength largely hinder their processing and applications. Here, we report a novel Co-free Fe47Cr16Ni26Ti6Al5 medium-entropy stainless steel (MESS) strengthened by high-density coherent L12 nanoprecipitates (NPs). We use a thermodynamic approach to pursuing a large volume fraction of stable L12 NPs in the coarse-grained face-centered-cubic (FCC)-structured matrix of the MESS, which is then readily fabricated through conventional casting and thermomechanical-treatment techniques. The MESS exhibits a high ultimate tensile strength of 1.35 gigapascals (GPa) and a great total elongation of 36% at room temperature (RT), evading the strength-ductility trade-off dilemma in conventional stainless steels. The high strength is mainly due to the chemical- ordering strengthening of high-density coherent L12 NPs. The ductile L12 NPs cooperative with the dynamic refinement of the deformation substructures endow the MESS with an excellent work-hardening ability and a large uniform ductility. Furthermore, the MESS maintains a high yield strength of ~ 0.8 GPa at 700 oC, which is better than many Fe-based superalloys and stainless steels, even comparable to some Ni-based superalloys. The steady-state creep rates at 750 oC are at least two orders of magnitude lower than those of conventional Ni-based superalloys and heat-resistant steels. The excellent creep resistance is achieved via the strong interactions between sliding dislocations and stable L12 NPs at elevated temperatures, which effectively impedes the dislocation movement. The present study has huge potential for designing cost-effective engineering MESSs with excellent mechanical performance for practical applications.
Influence of the leaf-cutting ant Atta sexdens Linnaeus (1758) on the taxonomic and functional diversity of woody plants in Atlantic Forest fragments
Authors: Garcia Jéssica Magon , Pereira Lya Carolina , Vasconcelos Heraldo Luís , Torezan José Marcelo Domingues , Rodrigues Larissa Cerqueira Dias 
Published: July 2023

Abstract

Leaf-cutting ants can act as a biotic filter, selecting plant traits according to foraging preferences, affecting plant community species composition. We evaluated the relationship between the floristic and functional diversity of woody plants in fragments of Semideciduous Seasonal Atlantic Forest (SAF) with the presence or absence of Atta sexdens (Linnaeus, 1758) nests. We expected that, in places where A. sexdens nests are present, floristic diversity is lower and the plant community is dominated by species characterized by more anti-herbivore defenses and lower nutritional content. We sampled nine SAF fragments in the northern Paraná state, Brazil. The diversity of woody regenerants (> 1m in height, <2cm diameter) was estimated and ten functional traits were evaluated: gap dependence, deciduousness, leaf nitrogen and carbon content, leaf C/N ratio, leaf trichomes, leaf thickness, specific leaf area, presence of latex and condensed tannins. Contrary to expectations, there were no differences in floristic diversity between sites with or without A. sexdens, and functional diversity was also similar, reflecting the floristic similarity. We suggest that the herbivory pressure by A. sexdens on the woody regenerants of SAF fragments is not strong enough to cause significant mortality in individual species and, consequently, affect plant diversity and composition. This may in part be related to the fact that most A. sexdens nests are established in the edges of the fragments and that ants from these nests also forage in the agricultural matrix. Longer-term studies are still needed to fully elucidate the effects of A. sexdens on Atlantic Forest fragments.
Mutual entity authentication of a quantum key distribution network system using authentication qubits
Authors: Park Hojoong , Park Byung Kwon , Woo Min Ki , Kang Min-Sung , Choi Ji-Woong , Kang Ju-Sung , Yeom Yongjin , Han Sang-Wook 
Published: July 2023

Abstract
Entity authentication is crucial for ensuring secure quantum communication because the identity of the participants in a network must be confirmed before transmitting any confidential information. We propose a practical entity authentication protocol, which uses authentication qubits, for quantum key distribution (QKD) network systems. In this protocol, authentication qubits encoded with pre-shared information are generated and exchanged to verify the legitimacy of each entity. Using the authentication qubit, participants can identify each other with the same level of security as in QKD through the quantum channel. The proposed protocol can be easily integrated into existing QKD systems without additional hardware. In this study, we demonstrate the efficacy of the proposed scheme using a 1 x N QKD network system, and verify its stable operation over a deployed fiber network. In addition, we present a security analysis of the proposed entity authentication protocol and architecture.
Performance of Soil-Bearing Spread Footings Supporting Highway Structures
Authors: Tarawneh Bashar , Nusairat Jamal , Sargand Shad , Merklin Christopher , White Kevin 
Published: July 2023

Abstract

Soil-bearing spread footings (SBSF) are becoming an appealing alternative to support highway structures. SBSF have many advantages compared to deep foundations, such as cost-saving, expedited construction, simple design, environmentally friendly, and less maintenance. This paper aims to evaluate highway structures' performance supported by SBSF at four sites in Ohio. This evaluation's outcomes are recommendations for future use of spread footings and potential limitations on their use. The project team reviewed the documented performance data, evaluated existing footings' performance, and compared them to the structural performance criteria. The team also examined the calculated settlement, provided an estimate of the tolerable structure settlement, compared the measured settlement to the predicted settlement, and compared performance to soil conditions. The collected data and the field review of all four sites included in this research showed that SBSF performed as designed, and there were no signs of settlements and/or rideability issues. SBSF were constructed following the manuals and procedures by the Ohio Department of Transportation (ODOT) and performed adequately. The paper provides recommendations for the method of settlement monitoring and the Ohio department of transportation plan notes.
No microorganism was detected in amniotic fluid of healthy pregnancies from the second trimester to the delivery
Authors: Liu Yu , Ma Jingmei , Li Xiang , Zhao Haijuan , Ai Qubo , Zhang Lanying , Tong Yulong , Meng Lingzhen , Yang Huixia 
Published: July 2023

Abstract

Background The early colonization and establishment of the microbiome in newborn is a crucial step in the development of immune system and host metabolism. However, the exact timing of initial microbial colonization remains a subject of ongoing debate. While numerous studies have attempted to determine the presence or absence of intrauterine bacteria, the majority of them have drawn conclusions based on sequencing data from maternal or infant samples taken at a single time point. In this study, we aimed to investigate the microbial signals in amniotic fluid (AF) from the second trimester until the time of delivery using multiple microbiological methods.
Methods AF samples were collected during the second trimester (19-21 gestational weeks) and at the time of delivery. Cohort 1 included 51 women who underwent the term and elective cesarean section, with both their second trimester and delivery AF samples (n=55, respectively) analyzed.Cohort 2 contained of 22 women who experienced preterm birth and/or infection, with only their second trimester AF samples (n=24) examined. Additionally, multiple procedural negative controls and technical positive controls were applied into this study to remove potential contamination. Microbial profiles were assessed through cultivation, quantitative real-time polymerase chain reaction, 16S ribosomal RNA gene sequencing, and cytokine analysis.
Results In cohort 1, the bacterial load and community structure in the second trimester AF samples were indistinguishable from negative controls. Although marginally higher bacterial loads and different bacterial communities were observed in the delivery AF samples compared to negative controls, these bacterial DNA signals were not considered biologically functional due to the absence of maternal inflammatory responses. In cohort 2, the bacterial load and community structure of the second trimester AF samples differed significantly from those of negative controls, with Ureaplasma and Lactobacillus identified as the most prevalent genera against negative controls.
Conclusions Our study demonstrates that no microorganisms were detected in the AF of healthy pregnancies from the second trimester to the delivery. The presence of Ureaplasma and Lactobacillusin the second trimester AF may be associated with preterm birth and intra-amniotic infection.
Can preoperative high-intensity strength training combined with balance training improve early outcomes after total knee arthroplasty?
Authors: Sun Jian-ning , Shan Yu-zhou , Wu Li-xia , Li Ning , Xu Fei-hu , Kong Xiang-ru , Zhang Bei 
Published: July 2023

Abstract
Background: To investigate the effect of preoperative high-intensity strength training combined with balance training on the knee function of end-stage knee osteoarthritis (KOA) patients after total knee arthroplasty (TKA).
Methods: A prospective study was conducted on end-stage KOA patients awaiting TKA. The patients were divided into an experimental group and a control group according to whether they received a preoperative training intervention. The differences in knee flexor-extensor strength, knee range of motion (ROM), timed up and go (TUG) test result, stair ascend/descend test result, Knee Society score (KSS) and Berg balance scale (BBS) score were assessed in both groups at baseline (T1), before operation (T2), 3 months after operation (T3), and 1 year after operation (T4).
Results: After high-intensity strength training and balance training, the knee flexor-extensor strength, TUG test result, stair ascend/descend test result, and KSS were all significantly improved at T2 in the experimental group over the control group. At T3, the knee ROM, knee flexor-extensor strength, TUG test result, BBS score, and KSS clinical and functional scores were all significantly superior in the experimental group. The experimental group enjoyed a superiority in KSS clinical and functional scores until T4. Group×time and between-group interactions were found in all assessment indicators in both groups (p<0.01).
Conclusion:Preoperative high-intensity strength training combined with balance training can enhance the knee flexor-extensor strength and balance of patients with end-stage KOA in the short term and help improve early outcomes after KOA.
Trial registration：（ChiCTR2000032857，2020-05-13）
SOX21-AS1 augmented cervical cancer growth by triggering FZD3 to activate the Wnt/β-catenin signaling pathway
Authors: Zhi Yanfang , Du Peipei , Li Ya , Liu Hui , Jiang Tao , Zhao Xin , Li Xiaofu 
Published: July 2023

Abstract
Long non-coding RNA (LncRNA) SOX21-AS1 has been reported that it plays an important role in biological processes of several cancers. However, how it functions in cervical cancer (CC) still remain unclear. This investigation seeks to explore the impact of SOX21-AS1 on CC cell proliferation, invasion and migration and its association to the FZD3 and the Wnt/β-catenin signaling pathway. SOX21-AS1 expression levels were detected using real-time quantitative PCR in 20 cases of cervical cancer together with its adjacent tissues and several cervical cancer cell lines. Transgenic technology and functional experiments were conducted to confirm the carcinogenic properties of SOX21-AS1, and western blot was utilized to analyze the regulatory network composed of SOX21-AS1, FZD3 and the Wnt/β-catenin signaling pathway in CC. Through bioinformatics analysis, we found that the expression of SOX21-AS1 in CC was the highest among 16 kinds of tumor tissues. Moreover, clinical specimens confirmed that both CC tissues and cell lines possessed elevated SOX21-AS1 expressions (P < 0.01). CC cells which stably expressed upregulated SOX21-AS1 were noted to possesses higher rates of metastasis, invasion and proliferation, lower apoptotic rates and higher expression of FZD3,β-catenin and c-myc(P < 0.01). Conversely, the use of small interfering RNA to inhibit the expression of SOX21-AS1 yielded the opposite results (P < 0.01). SOX21-AS1 functions as an oncogenic lncRNA which enhances CC cell metastasis, invasion and proliferation through FZD3 upregulation via Wnt/β-catenin signaling pathway activation. This lncRNA may represent an important biomarker for CC patients.
The optimization model of UAV shooting flight routes
Authors: ZHENG TING , li shangze , xiao zhensheng , zhang luyan 
Published: July 2023

Abstract
For the first drone operator, it is difficult to master the techniques of flight altitude, flight speed and shooting angle. The main task of this paper is to analyze how to adjust the flying height, flight speed and shooting angle of the drone and use the drone to take satisfactory photos. Mainly complete the following four questions. In the first part, we analyze the geometric relationship between the flying height and the camera's acquisition range area. Then, we set a pixel accuracy score index as the criterion. The sum of the weighted capture area and the pixel accuracy score is taken as the objective function, the flight height does not exceed the height limit area, and the unit pixel point is greater than 800 as the constraint condition. An optimization model for the accuracy of drone shooting was established. We use computer simulation to solve the accuracy method step by step. We obtain that the height range of the drone is [25 m, 100 m], and the optimal aerial height is 33 m when θ = 60º. In the second part, based on the first part, we independently analyzed the numerical relationship between the shooting angle and the camera acquisition range area. The sum of the captured area and the pixel accuracy score after updating the weight is the objective function, the shooting angle is the decision variable, and the UAV shooting accuracy score optimization model is used. The golden section algorithm is used to solve this problem. When the flying speed of the drone is 40 m/s and the flying height is 33 m, the optimal range of the shooting angle of the drone is [56.2248º, 77.4972º]. In the third part, we established a small UAV system model and a ground target model based on the knowledge of robotics. We generated motion trajectories of discrete and continuous states of the object. The recursive target tracking algorithm is used to continuously adjust the UAV shooting angle to ensure that the target is within its shooting area. Finally, the corresponding values of the two shooting angles with respect to time are obtained. The range of angle changes is [42.18º, 79.87º] and [32.81º, 79.89º]. In the fourth part, we generated 1 skyscraper, 2 signal towers, 3 telephone poles, 3 residential buildings, for a total of 9 obstacles. We present a new RRT* algorithm for path planning based on the obstacles encountered by UAVs during flight, that is, using the cost function to select the node with the minimum cost in the field of expanding nodes as the parent node, and the two new nodes are recalculated. We use MATLAB to select a reasonable obstacle avoidance strategy, and the global optimal route is obtained by smoothing processing. Finally, we verify the rationality of the model. Through computer simulation, we determine the three-dimensional field of flight speed constant, flying height and shooting angle with respect to the score, which proves model stability and robustness.
Age-dependent Robust Strategic Asset Allocation with Inflation-Deflation Hedging Demand
Authors: Kusuda Koji , Kikuchi Kentaro 
Published: July 2023

Abstract
This study analyzes robust strategic asset allocation under a quadratic security market model with stochastic volatility and inflation rates assuming “homothetic age-dependent robust utility” in which relative ambiguity aversion is a decreasing function of age. We consider the finite-time consumption-investment problem and derive a linear approximate optimal robust portfolio decomposed into myopic, intertemporal hedging, and inflation-deflation hedging demands. Our numerical analysis of equity allocations shows modest hump-shaped age effects, similar to the results of a previous empirical analysis, and that the upswing is due to the increase in myopic demand, while the downswing is due to the decline in intertemporal hedging demand.
JEL Classification C61 · G11
Genetic features of young and aged animals after peripheral nerve injury: implications for diminished regeneration capacity
Authors: huang weixiao , Yi Sheng , zhao lili 
Published: July 2023

Abstract
The spontaneous regeneration capacity of peripheral nerves is fundamentally reduced with advancing age, leading to severe and long-term functional loss. The cellular and molecular basis underlying incomplete and delayed recovery of aging peripheral nerves is still murky. Here, we collected sciatic nerves of aged rats at 1d, 4d, and 7d after nerve injury, systematically analyzed the transcriptional changes of injured sciatic nerves, and examined the differences of injury responses between aged rats and young rats. RNA sequencing revealed that sciatic nerves of aged and young rats exhibit distinctive expression patterns after nerve injury. Acute and vigorous immune responses, including motivated B cell receptor signaling pathway, occurred in injured sciatic nerves of both aged and young rats. Different from young rats, aged rats have CD8+ T cells both in normal and injured state and the elevation of M2 macrophages seemed to be more robust in sciatic nerves, especially at later time points after nerve injury. Young rats, on the other hand, showed strong and early up-regulation of cell-cycle related genes. These identified unique transcriptional signatures of aged and young rats help the understanding of aged-associated injury responses in the wound microenvironments and provide essential basis for the treatment of regeneration deficits in aged population.
Network analysis challenges the functional importance of charismatic and commercial species in the Gulf of California food web
Authors: Olmo-Gilabert Ruben , Cruz-Agüero Gustavo De La , Scotti Marco , Cruz-Escalona Víctor H. , Navia Andrés Felipe 
Published: July 2023

Abstract
Ecosystem-based management for marine life conservation is of growing consensus, although the focus on protecting either charismatic or commercially important species still persist, understating species playing key functional roles. Here we quantified the topological importance of species with high economic or conservation value by simulating extinction scenarios in the Gulf of California trophic network. We found food web robustness against removals of charismatic or commercially important species. Instead, other species suggest strong roles on food web functioning. Invertebrates such as squids and crustaceans are in crucial intermediate positions, making them key bottlenecks for energy transfers. A mixed ecosystem control is at play in the food web, which is dominated by species controlling the indirect effects in top-down forcing as white sharks and hammerheads and bottom-up forcing imposed by the phytoplankton. Direct effect transmission is regulated by the silky shark due to its diverse diet. Only two charismatic removals, blue whale and white shark affected the closeness centrality patterns, whereas only blue shrimp and Monterrey sardine are commercially important species with functional relevance roles in the food web. We hope our network analysis will help decision makers in the development of novel conservation policies. Our results highlight in fact the importance of loliginid squid and blue shrimp populations, having beneficial indirect effects for the protection of charismatic species. This study is particularly timing since islands and protected areas of the Gulf of California were upgraded to the highest level of concern by the UNESCO World Heritage Convention in 2020.
Effect of Electrical Muscular Stimulation on Occupational Activates: a Systematic Review and Meta-Analysis
Authors: Eldaly Sameh 
Published: July 2023

Abstract
Objective: To determine the effectiveness of electrotherapy modalities to improve muscular performance and coordination in occupational activities.
Data sources:: Search strategies were conducted on 5 Randomized control trial and one Pilot study and one Experimental proof.
Results: seven studies (151 participants) were analyzed. Movement therapy plus electrical stimulation for at least two weeks is likely to produce the best outcome in improving muscle recruitment and coordination patients with in patients with occupational activities disorders.
Conclusion: the available evidence suggests that the electrical muscular stimulation in occupational therapy can be a useful adjunct to traditional therapy approaches for improving muscle function, strength, and physical function in a variety of populations.