bluuebunny/crossref_metadata_2025_split huggingface dataset:
Investigating the Intentions and Reasons of Senior High School Students in Registering for Nursing Education in China
Authors: Zhang Zhong , Yang Chaoqun , Wang Ying , Deng Guoying , Chang Jian 
Published: June 2023

Abstract
Background
Shortage of qualified nurses and their low level of educational qualifications hinders the development of global health services. Researches have proved the role of nursing education in addressing these problems. However, no-related studies have focused on senior high school students before in China. This study aimed to explore senior high school students’ intention to learn nursing and identify the factors influencing their decision-making process.
Methods
An anonymous questionnaire was distributed to 8050 senior high school students. The questionnaire that included questions regarding their demographic characteristics, understanding of the nursing specialty, cognition of the nurse occupation and experiences during the pandemic. Descriptive calculation, the chi-square test and logistic regression were used for the analysis.
Results
Only 0.73% of the participants had a clear intention to study nursing. Academic performance and family support were significant predictor of students’ intentions to pursue nursing education. Students’ interest in nursing specialty were associated with their choice. There was a positive correlation between cognition of nursing occupation and students’ choice of nursing. Students’ experience of Covid-19 also have positive impact on their nursing career choice.
Conclusion
The shortage of nurses is a particularly daunting challenge in China. This study provided a new perspective for predictors of the nursing shortage and the potential interventions.
Pharmacogenomic Overlap Between Antidepressant Treatment Response in Major Depression &amp; Antidepressant Associated Treatment Emergent Mania in Bipolar Disorder
Authors: Nunez Nicolas , Coombes Brandon , Beaupre Lindsay Melhuish , Ozerdem Aysegul , Resendez Manuel Gardea , Romo-Nava Francisco , Bond David , Veldic Marin , Singh Balwinder , Moore Katherine , Betcher Hannah , Kung Simon , Prieto Miguel , Fuentes Manuel , Ercis Mete , Miola Alessandro , Ruiz Jorge Sanchez , Jenkins Gregory , Batzler Anthony , Leung Jonathan , Cuellar-Barboza Alfredo , Tye Susannah , McElroy Susan L. , Biernacka Joanna , Frye Mark 
Published: June 2023

Abstract
There is increasing interest in individualizing treatment selection for more than 25 regulatory approved treatments for major depressive disorder (MDD). Despite an inconclusive efficacy evidence base, antidepressants (ADs) are prescribed for the depressive phase of bipolar disorder with oftentimes an inadequate treatment response and or clinical concern for mood destabilization. This study explored the relationship between antidepressant response in MDD and antidepressant-associated treatment emergent mania (TEM) in bipolar disorder (BD). We conducted a genome-wide association study (GWAS) and polygenic score analysis of TEM and tested its association with a subset of BD-I patients treated with SSRIs or SNRIs. Our results did not identify any genome-wide significant variants although, we found that a higher polygenic score (PGS) for antidepressant response in MDD was associated with higher odds of TEM in BD. Future studies with larger transdiagnostic cohorts treated with antidepressants are encouraged to identify a neurobiological mechanism associated with a spectrum of depression improvement (response- emergent mania).
Soil microbial improvement using enriched vinasse as a new abundant waste
Authors: Kariminia Tahereh , Rowshanzamir Mohammad A. , Abtahi S. Mahdi , Soleimanian-Zad Sabihe , Bak Hamid Mortazavi , Baghbanan Alireza 
Published: June 2023

Abstract
This study proposes the use of vinasse, an inexpensive and readily available waste biopolymer, as a fundamental component of a waste culture medium that can enhance the effectiveness and cost-efficiency of the Microbial-Induced Calcite Precipitation (MICP) method for sustainable soil improvement. Vinasse enriched with urea, sodium caseinate, or whey protein concentrate is employed to optimize bacterial growth and urease activity using Taguchi Design of Experiments (TDOE) and statistical analysis. The results show that using the new culture medium yields a 91 times reduction in the cost of culture medium compared to conventional Nutrient Broth (NB) culture medium while improving soil shear strength parameters. Furthermore, the recycling of vinasse as a waste product can promote the progress of a circular economy and reduce environmental pollution. This study provides a promising approach to achieving cost-effective and sustainable soil improvement using waste biopolymers.
CRISPR-based gene drives generate super-Mendelian inheritance in the disease vector Culex quinquefasciatus.
Authors: Harvey-Samuel Tim , Feng Xuechun , Okamoto Emily , Purusothaman Deepak-Kumar , Leftwich Philip , Alphey Luke , Gantz Valentino 
Published: June 2023

Abstract
Culex mosquitoes pose a significant public health threat as vectors for a variety of diseases including West Nile virus and lymphatic filariasis, and transmit pathogens threatening livestock, companion animals, and endangered birds. Rampant insecticide resistance makes controlling these mosquitoes challenging and necessitates the development of new control strategies. Gene drive technologies have made significant progress in other mosquito species, although similar advances have been lagging in Culex. Here we test the first CRISPR-based homing gene drive for Culex quinquefasciatus, demonstrating the possibility of using this technology to control Culex mosquitoes. Our results show that the inheritance of two split-gene-drive transgenes, targeting different loci, are biased in the presence of a Cas9-expressing transgene although with modest efficiencies. Our findings extend the list of disease vectors where engineered homing gene drives have been demonstrated to include Culex alongside Anopheles and Aedes, and pave the way for future development of these technologies to control Culex mosquitoes.
WITHDRAWN: Hecubine suppresses lipopolysaccharide-stimulated neuroinflammation and oxidative stress by targeting TREM2 and regulating Nrf2/TLR4 signaling in vitro and in vivo
Authors: Li Lin , Xu Nan , Wang Xiu-Fen , He Yu-Lin , Tang Ben-Qin , Simon Ming-Yuen 
Published: June 2023

Abstract
Neuroinflammation and oxidative stress play crucial roles in many neurological diseases of the central nervous system. Targeting key proteins in inflammatory signaling may provide a new therapy for neuroinflammation. Hecubine is an active monoterpene indole alkaloid found in Ervatamia officinalis and the majority of its biological activities have not yet been explored. In the present study, we investigated the effects and mechanism of Hecubine on LPS-mediated neuroinflammation in vivo and in vitro for the first time. The results demonstrated that Hecubine reduced LPS-stimulated inflammatory cytokines overexpression, activated TREM2 expression, as well as suppressed the levels of TLR4-, MyD88-, and NF-κB-related proteins in BV2 microglia cells. Hecubine also exhibited an antioxidative effect, as evidenced by the reduction of ROS production and activation of the Nrf2/HO-1 pathway. Further drug target identification revealed that TREM2 is a primary interacting target of Hecubine. Knockdown of TREM2 mRNA expression significantly abolished Hecubine-induced anti-inflammatory and antioxidative effects via the upregulation of TLR4 signaling and the downregulation of Nrf2 pathway proteins. In vivo, after injection of LPS into the brain of zebrafish larvae, Hecubine administration obviously rescued behavioral deficits, inhibited the expression of pro-inflammatory cytokines, and prevented oxidative stress by activating TREM2. Taken together, Hecubine directly targets TREM2 to reduce neuroinflammation and oxidative stress and serves as a potential therapeutic agent for the treatment of neural inflammation-associated CNS diseases.
Laparoscopic Boari flap for repair of long-segment ureteral avulsion or ureteral stricture: our experience with seventeen cases
Authors: Luo Peng , Deng Jiyi , Wu Xin , Luo Lianmin , Li Shaoquan , Long Quanwang , Wang Wei , Hu Zhengbang , Chen Weimin 
Published: June 2023

Abstract
Objective: This study was designed to evaluate the safety and clinical efficacy of using laparoscopic Boari flap for repair of long-segment ureteric avulsion or ureteric stricture of the middle and lower ureters,and to summarize the experiences.We can providing a safe and viable treatment option for patients with middle and lower ureteral defects.
Methods: We retrospectively collected data from seventeen patients who underwent laparoscopic Boari flap for repair of long-segment ureteral defects between October 2018 and March 2023,six of whom underwent Boari flap-psoas hitch,and assess patient demographic characteristics,intraoperative variables,postoperative complications,and follow-up outcomes.
Results: The median length of ureteral avulsion or ureteral stricture was 8 cm(range,3-16 cm).The median operative time was 180 min(range,155-240min).The median estimated blood loss was 150mL(range,20-200 mL). The median postoperative hospital stay was 9 days(range,7-17 days),with no major procedure-related complications in the perioperative period.Postoperative follow-up (rang,1-65months) CTU or ultrasonography showed that one patient developed ureteral stricture and severe hydronephrosis 1 year after surgery,and the hydronephrosis improved 3 months after placing 2 double J-tubes.The remaining sixteen patients showed no significant complications on postoperative review.
Conclusion: Laparoscopic Boari flap for repair of long-segment ureteric avulsion or ureteric stricture of the middle and lower ureters has good safety and feasibility.For ureteral avulsion,we prefer the psoas minor tendon as the recommended anchor for bladder suspension to avoid the femoral or genitofemoral nerve and the length-to-base ratio of the flap was up to 4:1 with good blood supply.
The phytohormones underlying the plant lateral root development in fluctuated soil environments
Authors: Xu Shengbao , Javed Sidra , Chai Xiangzheng , Wang Xiaoming 
Published: June 2023

Abstract
Background
Plants rely heavily on the formation of post-embryonic organs, such as leaves, branches, and lateral roots, to adapt to varying soil conditions. Lateral roots (LR) play a crucial role in the plant root system, serving as the primary factor in the exploration of water and nutrients in the soil. The development of LR is meticulously controlled by phytohormones to response the information obtained from the surrounding soil. This regulation ensures an optimal arrangement of lateral roots, enabling efficient nutrient absorption and adaptation to the challenging environmental conditions.
Scope
This review summarizes recent progress in understanding the mechanisms of lateral root layout in different soil micro-environments and the role of phytohormones in mediating LR development for soil adaptation.
Conclusions
The intricate signaling network governing LR layout involves interactions among various soil factors, which are mediated by phytohormones. Despite its complexity, recent studies have yielded significant insights that can be applied to optimize LR arrangement in soil profiles through genetic and cultivation methods to enhance crop yield and stress tolerance.
Vitamin D status in hospitalized COVID‑19 patients is associated with disease severity and IL-5 production
Authors: Qiu Yali , Bao Wuping , Tian Xue , Zhang Yingying , Pan Yilin , Xie Guogang , Bao Aihua , Yin Dongning , Zhang Min , Zhou Yan 
Published: June 2023

Abstract
Background
There are many studies on relationship between vitamin D and coronavirus disease 2019 (COVID-19), while the results are matters of debate and the mechanisms remain unknown. The present study to assess the impact of serum 25-hydroxyvitamin D [25(OH)D] levels on the severity of disease in hospitalized COVID-19 patients and identify potential mechanisms.
Methods
A total of 399 hospitalized COVID-19 patients were recruited from three centers between December 19, 2022, and February 1, 2023.
Results
Levels of 25(OH)D were significantly lower in the deceased group than other three groups (P < 0.05). The levels of 25(OH)D (odds ratio = 0.986, 95% confidence interval: 0.973–0.998, P = 0.024) and IL-5 (odds ratio = 1.239, 95% confidence interval: 1.104–1.391, P = 0.04) were independent risk factors for the severity of COVID-19 disease upon admission. Serum 25(OH)D levels combined with IL-5 levels and eosinophil (Eos) counts were able to predict the mortality of patients with COVID-19. Circulating 25(OH)D status correlated negatively with the expression of IL-5 (r=-0.262, P < 0.001) and was positively linked with CD8+ T cell counts (r=-0.121, P < 0.05) in patients with COVID-19.
Conclusions
Most COVID-19 patients have vitamin D deficiency and a severe deficiency is associated with fatal outcomes. This study found that the serum 25(OH)D status in COVID-19 patients correlated negatively with the expression of IL-5. The specific mechanism for this relationship is worth further exploration.
Trial registration
This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-ncnd/4.0/).
Systemic sclerosis and risk of bronchiectasis: A nationwide longitudinal cohort study
Authors: Yang Bumhee , Kim Bo-Guen , Han Kyungdo , Jung Jin-Hyung , Kim Ji Hyoun , Park Dong Won , Kim Sang-Heon , Kim Eung-Gook , Sohn Jang Won , Yoon Ho Joo , Choi Hayoung , Lee Hyun 
Published: June 2023

Abstract
Backgroud: The impact of systemic sclerosis on the development of bronchiectasis is unclear. This study aimed to compare the risk of bronchiectasis between individuals with systemic sclerosis and those without using a nationwide longitudinal dataset.
Methods: Using the Korean National Health Insurance Service dataset between 2010 and 2017, we identified 4,845 individuals aged ≥ 20 years with systemic sclerosis and 24,225 without systemic sclerosis who were matched 1:5 by age and sex. They were followed up until the date of a bronchiectasis diagnosis, death, or December 31, 2019, whichever came first.
Results: During a median follow-up period of 6.0 (interquartile range, 3.2–8.7) years, 5.3% of the systemic sclerosis cohort and 1.9% of the matched cohort developed bronchiectasis, with incidence rates of 9.99 and 3.23 per 1,000 person-years, respectively. Even after adjusting for potential confounders, the risk of incident bronchiectasis was significantly higher in the systemic sclerosis cohort than in the matched cohort (adjusted hazard ratio 2.63, 95% confidence interval 2.22–3.12). The risk of incident bronchiectasis was notably higher in younger individuals (P for interaction = 0.048) and those with coexisting connective tissue disease (Pfor interaction = 0.005) compared to their counterparts.
Conclusions: The risk of incident bronchiectasis is higher in individuals with systemic sclerosis than those without. Bronchiectasis should be considered one of the pulmonary manifestations related to systemic sclerosis.
Adaptive Community Aware Graph Network for Item Recommendation
Authors: Hao Pengyi , Qian Zhaojie , Wang Shuang , Bai* Cong 
Published: June 2023

Abstract
Due to the heterogeneity of a large amount of real-world data, meta-paths are widely used in recommendation. Such recommendation methods can represent composite relationships between entities, but cannot explore reliable relations between nodes and influence among meta-paths.
For solving this problem, adaptive community aware graph network (ACAGN) is proposed in this paper for item recommendation. By dynamically adapting the shape of communities and simultaneously calculating the similarity between them, the similarity of communities is fully learnt among nodes in meta-path based subgraphs. The semantic features of users and items are used to jointly learn weights between nodes, further to get reliable node relations, resulting in robust embedding. Finally, the embeddings of users and items are fed to extend matrix factorization for getting the top recommendations. A series of comprehensive experiments are conducted on two different public datasets. The results show that ACAGN is a better way to do item recommendation compared with the state-of-the-art methods.
Bioinformatics analysis of the shared pathogenesis of psoriasis and Crohn's disease
Authors: Yu Yao , Liu Yaoguang , Ni Zhizhan , Ma Shaoze , Zhou Jinzhe 
Published: June 2023

Abstract
Background
There exists a bidirectional causal relationship between psoriasis and Crohn's disease, where psoriasis can increase the risk of Crohn's disease, and Crohn's disease can also increase the risk of psoriasis. However, the underlying mechanism of their co-occurrence remains unclear. This study aims to explore the pathogenesis of psoriasis combined with Crohn's disease through bioinformatics analysis.
Methods
Psoriasis skin tissue data (GSE117239) and Crohn's disease intestinal tissue data (GSE95095) were downloaded from the GEO database. The imma R package and Weighted Gene Co-expression Network Analysis (WGCNA) were used to identify common differentially expressed genes. Further analyses included Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis, construction of a protein-protein interaction (PPI) network, screening and validation of hub genes, prediction and validation of hub transcription factors (TFs), and additional analysis of the diagnostic value of hub genes, as well as prediction of therapeutic drugs.
Results
99 common differentially expressed genes were identified for psoriasis and Crohn's disease. The results of GO and KEGG enrichment analysis were focused on inflammation and energy metabolism processes. 6 hub genes were screened, namely MMP9, CXCR2, CXCL1, CXCL10, HMGCS2, and PPARGC1A. Some of these hub genes showed high diagnostic value. Three hub TFs were predicted and validated, which were STAT1, STAT3, and IRF1. Based on these hub genes and hub TFs, a total of 66 drugs were predicted, with some drugs overlapping with the existing therapeutic drugs for psoriasis or Crohn's disease.
Conclusions
This study revealed the potential common pathogenesis of psoriasis and Crohn's disease through bioinformatics analysis. These hub genes, hub TFs, and predicted drugs may provide new perspectives for further mechanistic research.
Explore the alterations of downstream molecular pathways caused by ARID1A mutation/knockout in human endometrial cancer cells
Authors: Xing Baoling , Zhang Xiaoying , Gu Xia , Xiang Lintao , Wang Cuiping , Jin Yueling 
Published: June 2023

Abstract
Purpose
As one of the most common gynecologic malignancies, endometrial cancer (EC) is driven by multiple genetic alterations that may be targeted for treatments. AT-rich interaction domain 1A (ARID1A) gene mutations were reported as early events in endometrial carcinogenesis.
Methods
To explore the alterations of downstream molecular pathways caused by ARID1A mutations and the associated therapeutic implications, we edited ARID1A gene in human endometrial cancer cell line Ishikawa using the Clustered Regularly Interspaced Short Palindromic Repeats and CRISPR-Associated Proteins (CRISPR/Cas9) technology. We successfully constructed a stable Ishikawa cell line with a confirmed 10bp deletion on the ARID1A gene, which resulted in a code-shift mutation and gene knockout.
Results
Compared with unedited wild-type cells, ARID1A knockout (KO) led to reduced apoptosis, accelerated transformation from G0/G1 to S phase and enhanced cell proliferation. In addition, ARID1A KO resulted in high levels of microsatellite instability (MSI-H). Moreover, transcriptomic analyses showed that ARID1A KO can lead to activated phosphoinositide 3-kinase (PI3K)/protein kinase B (Akt) signaling. Furthermore, experimental analyses demonstrated that ARID1A KO cells had reduced expression of genetic instability-associated markers mutL homologue 1 (MLH1) and progesterone receptor B (PR), and increased p-Akt expression.
Conclusion
These findings support further exploration of ARID1A as a therapeutic target for EC, and provide insights in developing more effective treatments in EC, such as the combinatory use of immune checkpoint inhibitors.
Hsa_circ_001726 axis contributes to metastasis of hepatocellular carcinoma
Authors: Ai Jiaoyu , Zhang Wanlin , Deng Wensheng , Yan Likun , Zhang Lidong , Huang Zongjing , Wu Ziyi , Ai Junhua , Jiang Hai 
Published: June 2023

Abstract
Hepatocellular carcinoma (HCC) is one of the most common malignant tumors worldwide. CircRNAs participate in the development of HCC. This work aims to search a key circRNA that plays an important role in HCC. The differentially expressed genes circRNAs in HCC tumor tissues was identified by mining GSE121714 dataset. We found that 11 circRNAs were up-regulated in tumor tissues of HCC patients with or without metastasis. Among them, hsa_circ_001726 was highly expressed in tumor tissues of HCC patients with or without metastasis, and associated with tumor size, TNM stages and poor prognosis of HCC patients. Hsa_circ_001726 expression was positively correlated with N-cadherin expression, and negatively correlated with E-cadherin expression, indicating that it was closely associated with epithelial mesenchymal transition (EMT) of HCC cells. Moreover, hsa_circ_001726 expression was increased in HCC cells. Hsa_circ_001726 elevated PRMT9 expression by sponging miR-671-5p, and then activated Notch signaling pathway. Additionally, hsa_circ_001726 deficiency repressed malignant phenotypes of HCC cells, including migration, invasion and EMT. In vivo, Xenograft mouse models and orthotopic transplantation tumor mouse models were constructed to verify the role of hsa_circ_001726 in growth and metastasis of HCC. Hsa_circ_001726 deficiency reduced tumor growth and metastasis of HCC. In conclusion, this work demonstrated that hsa_circ_001726 activated Notch signaling pathway via miR-671-5p/PRMT9 axis, thereby accelerating malignant progression and metastasis of HCC. Therefore, hsa_circ_001726 may be a biomarker for diagnosis and prognosis of HCC.
A case study on oil spills, coastal pollution, and the development of antimicrobial resistance in bacteria isolated from soil collected along the Arabian Sea and Bay of Bengal coastlines
Authors: Rekadwad Bhagwan Narayan , Shouche Yogesh S. , Jangid Kamlesh 
Published: June 2023

Abstract
Pelagic transport causes oil pollution by traversing the open ocean in southern Asia and Indian territory via international tanker routes. The natural flow of ocean water from east to west, which carries pollutants and biohazards into the marine waters belonging to the Indian territory, is what causes the deposition and saturation of oil spills, tar (i.e., hydrocarbons), and wastes in ocean waters. Water currents and tides carry tons of crude oil that has leaked from oil tanker accidents in the open ocean to coastlines. Here we report that marine traffic of sailing flags across oceans causes pollution that results in the formation of algal blooms and has an impact on the production of chlorophyll and the mortality of fauna. The consequences of pollution have mostly affected coastal areas, especially mangroves, and may destroy potential fishing zones. Furthermore, it was recorded that long-term exposure to pollutants may cause the development of antibiotic and hydrocarbon resistance in bacteria. Bacterial taxa like Bacillus, Domibaciluus, Acinetobacter, Brevundimonas, Streptococcus, Mycolibacterium, and Spingomonas, among others, were found, and their identities were confirmed by sequencing the 16S rRNA gene. Antimicrobial resistance in these bacteria may pose a threat to public health.