bluuebunny/crossref_metadata_2025_split huggingface dataset:
Evaluation of efficacy and safety for compound kushen injection combined with intraperitoneal chemotherapy for patients with malignant ascites: A systematic review and meta-analysis
Authors: Yu Hui-Bo , Hu Jia-Qi , Han Bao-Jin , Cao Hui-Juan , Chen Shun-Tai , Chen Xin , Xiong Hong-Tai , Gao Jin , Du Yan-Yuan , Zheng Hong-Gang 
Published: March 2023

Objectives: Compound Kushen injection (CKI) combined with intraperitoneal chemotherapy (IPC) is widely used in the treatment of malignant ascites (MA). However, evidence about its efficacy and safety remains limited. This review aimed to evaluate the efficacy and safety of CKI combined with IPC for the treatment of MA.Methods: Protocol of this review was registered in PROSPERO (CRD42022304259). Randomized controlled trials (RCTs) on the efficacy and safety of IPC with CKI for the treatment of patients with MA were searched through 12 electronic databases and 2 clinical trials registration platforms from inception until 20 January 2023. The Cochrane risk-of-bias tool was used to assess the quality of the included trials through the risk of bias assessment. We included RCTs that compared IPC single used or CKI combined with IPC for patients with MA schedule to start IPC. The primary outcome was identified as an objective response rate (ORR), while the secondary outcomes were identified as the quality of life (QoL), survival time, immune functions, and adverse drug reactions (ADRs). The Revman5.4 and Stata17 software were used to calculate the risk ratio (RR) at 95% confidence intervals (CI) for binary outcomes and the mean difference (MD) at 95% CI for continuous outcomes. The certainty of the evidence was assessed according to the GRADE criteria.Results: A total of 17 RCTs were assessed, which included 1200 patients. The risk of bias assessment of the Cochrane risk-of-bias tool revealed that one study was rated high risk and the remaining as unclear or low risk. Meta-analysis revealed that CKI combined with IPC had an advantage in increasing ORR (RR = 1.31, 95% CI 1.20 to 1.43, p &lt; 0.00001) and QoL (RR = 1.50, 95% CI 1.23 to 1.83, p &lt; 0.0001) when compared with IPC alone. Moreover, the combined treatment group showed a lower incidence of myelosuppression (RR = 0.51, 95%CI 0.40–0.64, p &lt; 0.00001), liver dysfunction (RR = 0.33, 95%CI 0.16 to 0.70, p = 0.004), renal dysfunction (RR = 0.39, 95%CI 0.17 to 0.89, p = 0.02), and fever (RR = 0.51, 95%CI 0.35 to 0.75, p = 0.0007) compared to those of the control group. The quality of evidence assessment through GRADE criteria showed that ORR, myelosuppression, and fever were rated moderate, renal dysfunction and liver dysfunction were rated low, and QoL and abdominal pain were rated very low.Conclusion: The efficacy and safety of CKI combined with IPC were superior to that with IPC alone for the treatment of MA, which indicates the potentiality of the treatment. However, more high-quality RCTs are required to validate this conclusion.Systematic Review Registration: [https://www.crd.york.ac.uk/prospero/display_record.php?ID=CRD42022304259], identifier [PROSPERO 2022 CRD42022304259].
Research hotspots and frotiers of stem cells in stroke: A bibliometric analysis from 2004 to 2022
Authors: Zhang Qi , Zeng Yuting , Zheng Shuqi , Chen Ling , Liu Haining , Chen Hui , Zhang Xiaofeng , Zou Jihua , Zheng Xiaoyan , Wan Yantong , Huang Guozhi , Zeng Qing 
Published: March 2023

Background: Stroke is one of the leading causes of mortality and permanent disability worldwide. However, the current stroke treatment has a limited effect. Therefore, a new treatment is urgently needed. Stem cell therapy is a cutting-edge treatment for stroke patients. This study aimed to gain better understanding of global stem cell trends in stroke via a bibliometric analysis.Methods: We used the Web of Science Core Collection to search pertinent articles about stem cells in stroke published between 2004 and 2022. Analysis was conducted using CiteSpace, VOSviewer, and the R package “bibliometrix” to identify publication outputs, countries/regions, institutions, authors/co-cited authors, journals/co-cited journals, co-cited references, and keywords.Results: A total of 6,703 publications were included in the bibliometric analysis. The total number of citations significantly and rapidly increased between 2004 and 2022, with the most pronounced growth pattern observed in the period of 2008–2009. In terms of authoritarian countries, the USA had the most publications among the countries. As for institutions and authors, the most prolific institution was the University of South Florida, followed by Oakland University and then Shanghai Jiao Tong University, and Chopp, M. and Borlongan, Cesario V, had the most output among the authors. Regarding the journals, Cell Transplantation had the highest publication, followed by Brain Research. As for references, “Mesenchymal stem cells as trophic mediators” was the most frequently cited (2,082), and the article entitled Neuronal replacement from endogenous precursors in the adult brain after stroke had the strongest burstiness (strength = 81.35). Emerging hot words in the past decade included “adhesion molecule,” “mesenchymal stromal cell,” “extracellular vesicle,” “pluripotent stem cells,” “signaling pathway,” “plasticity,” and “exosomes.”Conclusion: Between 2004 and 2022, the terms “neurogenesis,” “angiogenesis,” “mesenchymal stem cells,” “extracellular vesicle,” “exosomes,” “inflammation,” and “oxidative stress” have emerged as the hot research areas for research on stem cells in stroke. Although stem cells exert a number of positive effects, the main mechanisms for mitigating the damage caused by stroke are still unknown. Clinical challenges may include complicating factors that can affect the efficacy of stem cell therapy, which are worth a deep exploration.
Intraoperative optical coherence tomography angiography for intraocular surgery
Authors: Cheng Dan , Zhang Ziyi , Qiao Yilin , Ye Yufeng , Zhu Xueying , Ruan Kaiming , Shen Meixiao , Liu Kaiyuan , Tao Jiwei , Chen Yiqi , Shen Lijun , Li Peng 
Published: March 2023

Purpose: To evaluate the feasibility of an intraoperative optical coherence tomography angiography (iOCTA) system integrated with a surgical microscope, and further investigate the real-time retinal vascular change in intraocular pressure fluctuation during surgery in rabbits.Methods: The feasibility of this system was confirmed through fundus angiography imaging in 12 rabbit eyes during simulated surgeries in 4 groups, 30 mmHg infusion pressure (IP), 60 mmHg IP, 100 mmHg IP, and control groups. The eyes were removed after seven days. Retinal ganglion cell (RGC) damage was analyzed histologically.Results: The OCTA technique can acquire images of retinal capillaries in rabbit eyes and quantitatively analyzing the vessel density. In the 30 mmHg IP group, there was no significant difference in the retinal vasculature during the operation. The OCTA signal and the RGCs decreased significantly in the 60 and 100 mmHg groups.Conclusions: The system can real-timely monitor the human retinal vasculature in high-resolution images and help surgeons to make decision during intraocular surgery.
Deep-learning based quantification model for hip bone marrow edema and synovitis in patients with spondyloarthritis based on magnetic resonance images
Authors: Zheng Yan , Bai Chao , Zhang Kui , Han Qing , Guan Qingbiao , Liu Ying , Zheng Zhaohui , Xia Yong , Zhu Ping 
Published: March 2023

Objectives: Hip inflammation is one of the most common complications in patients with spondyloarthritis (SpA). Herein, we employed use of a deep learning-based magnetic resonance imaging (MRI) evaluation model to identify irregular and multiple inflammatory lesions of the hip.Methods: All of the SpA patients were enrolled at the Xijing Hospital. The erythrocyte sediment rate (ESR), C-reactive protein (CRP), hip function Harris score, and disease activity were evaluated by clinicians. Manual MRI annotations including bone marrow edema (BME) and effusion/synovitis, and a hip MRI scoring system (HIMRISS) assessment was performed by experienced musculoskeletal radiologists. The segmentation accuracies of four deep learning models, including U-Net, UNet++, Attention-Unet, and HRNet, were compared using five-fold cross-validation. The clinical agreement of U-Net was evaluated with clinical symptoms and HIMRISS results.Results: A total of 1945 MRI slices of STIR/T2WI sequences were obtained from 195 SpA patients with hip involvement. After the five-fold cross-validation, U-Net achieved an average segmentation accuracy of 88.48% for the femoral head and 69.36% for inflammatory lesions, which are higher than those obtained by the other three models. The UNet-score, which was calculated based on the same MRI slices as HIMRISS, was significantly correlated with the HIMRISS scores and disease activity indexes (p values &lt;0.05).Conclusion: This deep-learning based automatic MRI evaluation model could achieve similar quantification performance as an expert radiologist, and it has the potential to improve the accuracy and efficiency of clinical diagnosis for SpA patients with hip involvement.
Nitrogen fertilization rates mediate rhizosphere soil carbon emissions of continuous peanut monoculture by altering cellulose-specific microbess
Authors: Wu Zhengfeng , Tang Zhaohui , Yu Tianyi , Zhang Jiancheng , Zheng Yongmei , Yang Jishun , Wu Yue , Sun Qiqi 
Published: March 2023

IntroductionCrops influence both soil microbial communities and soil organic carbon (SOC) cycling through rhizosphere processes, yet their responses to nitrogen (N) fertilization have not been well investigated under continuous monoculture.MethodsIn this study, rhizosphere soil microbial communities from a 5-year continuous mono-cropped peanut land were examined using Illumina HighSeq sequencing, with an N fertilization gradient that included 0 (N0), 60 (N60), 120 (N120) and 180 (N180) kg hm−2. Soil respiration rate (Rs) and its temperature sensitivity (Q10) were determined, with soil carbon-acquiring enzyme activities assayed.Results and discussionThe obtained results showed that with N fertilization, soil mineral N (Nmin) was highly increased and the soil C/N ratio was decreased; yields were unchanged, but root biomass was stimulated only at N120. The activities of β-1,4-glucosidase and polyphenol oxidase were reduced across application rates, but that of β-1,4-cellobiohydrolase was increased only at N120. Bacterial alpha diversity was unchanged, but fungal richness and diversity were increased at N60 and N120. For bacterial groups, the relative abundance of Acidobacteria was reduced, while those of Alphaproteobacteria and Gammaproteobacteria were increased at N60 and N120. For fungal members, the pathogenic Sordariomycetes was inhibited, but the saprotrophic Agaricomycetes was promoted, regardless of N fertilization rates. RDA identified different factors driving the variations in bacterial (root biomass) and fungal (Nmin) community composition. N fertilization increased Rs slightly at N60 and significantly at N120, mainly through the promotion of cellulose-related microbes, and decreased Rs slightly at N180, likely due to carbon limitation. N fertilization reduced microbial biomass carbon (MBC) at N60, N120 and N180, decreased SOC at N120 and N180, and suppressed dissolved organic carbon (DOC) at N180. In addition, the unchanged Q10 may be a joint result of several mechanisms that counteracted each other. These results are of critical importance for assessing the sustainability of continuously monocultured ecosystems, especially when confronting global climate change.
Effects of seasonal temperature regimes on embryo growth and endogenous hormones of Taxus chinensis var. mairei seeds
Authors: Zhou Man , Xu Yan , Wang Fang , Yang Xuejun , Lu Shunbao , Zhang Yanjie 
Published: March 2023

Seed dormancy is a mechanism that prevents seeds from germinating at times of the year when conditions are unfavorable, that is, when the chance of seed survival is low. Determining the seasonal dynamics of seed dormancy is important for exploring how plant regeneration is adapted to the environment. We studied the seed dormancy status of Taxus chinensis var. mairei, an endangered species in China, under simulated seasonal temperature regimes. The embryo length, embryo-to-seed (E : S) ratio, and percentage of seeds with a split seed coat increased when seeds were stratified at spring and autumn temperature regimes. The abscisic acid (ABA) content decreased during stratification at simulated seasonal temperatures, but no obvious pattern in the content of gibberellic acid (GA) and indole acetic acid (IAA) was observed. The GA–ABA and IAA–ABA ratios increased during stratification. These results suggest that T. chinensis var. mairei seeds have morphophysiological dormancy, and that the seasonal dynamics of seed dormancy break are controlled by endogenous hormones and their balances, which was confirmed by the results of a field experiment. Our study provides useful information for understanding the natural population regeneration and propagation of this threatened species.
Short-term transcriptomic analysis at organ scale reveals candidate genes involved in low N responses in NUE-contrasting tomato genotypes
Authors: Sunseri Francesco , Aci Meriem Miyassa , Mauceri Antonio , Caldiero Ciro , Puccio Guglielmo , Mercati Francesco , Abenavoli Maria Rosa 
Published: March 2023

BackgroundUnderstanding the complex regulatory network underlying plant nitrogen (N) responses associated with high Nitrogen Use Efficiency (NUE) is one of the main challenges for sustainable cropping systems. Nitrate (NO3-), acting as both an N source and a signal molecule, provokes very fast transcriptome reprogramming, allowing plants to adapt to its availability. These changes are genotype- and tissue-specific; thus, the comparison between contrasting genotypes is crucial to uncovering high NUE mechanisms.MethodsHere, we compared, for the first time, the spatio-temporal transcriptome changes in both root and shoot of two NUE contrasting tomato genotypes, Regina Ostuni (high-NUE) and UC82 (low-NUE), in response to short-term (within 24 h) low (LN) and high (HN) NO3- resupply. ResultsUsing time-series transcriptome data (0, 8, and 24 h), we identified 395 and 482 N-responsive genes differentially expressed (DEGs) between RO and UC82 in shoot and root, respectively. Protein kinase signaling plant hormone signal transduction, and phenylpropanoid biosynthesis were the main enriched metabolic pathways in shoot and root, respectively, and were upregulated in RO compared to UC82. Interestingly, several N transporters belonging to NRT and NPF families, such as NRT2.3, NRT2.4, NPF1.2, and NPF8.3, were found differentially expressed between RO and UC82 genotypes, which might explain the contrasting NUE performances. Transcription factors (TFs) belonging to several families, such as ERF, LOB, GLK, NFYB, ARF, Zinc-finger, and MYB, were differentially expressed between genotypes in response to LN. A complementary Weighted Gene Co-expression Network Analysis (WGCNA) allowed the identification of LN-responsive co-expression modules in RO shoot and root. The regulatory network analysis revealed candidate genes that might have key functions in short-term LN regulation. In particular, an asparagine synthetase (ASNS), a CBL-interacting serine/threonine-protein kinase 1 (CIPK1), a cytokinin riboside 5’-monophosphate phosphoribohydrolase (LOG8), a glycosyltransferase (UGT73C4), and an ERF2 were identified in the shoot, while an LRR receptor-like serine/threonine-protein kinase (FEI1) and two TFs NF-YB5 and LOB37 were identified in the root. DiscussionOur results revealed potential candidate genes that independently and/or concurrently may regulate short-term low-N response, suggesting a key role played by cytokinin and ROS balancing in early LN regulation mechanisms adopted by the N-use efficient genotype RO.
When fate hands you lemons: A moderated moderation model of bullying victimization and psychological distress among Chinese adolescents during floods and the COVID-19 pandemic
Authors: Zhang Yuchi , Jia Xiaoyu 
Published: March 2023

BackgroundBullying is a major problem worldwide and has numerous detrimental effects on the mental health of victims. The link between bullying and psychological distress in adolescents is well known. However, few studies have analyzed the impact of combined interpersonal, peer, and cultural factors on psychological distress using a social-ecological system framework in Eastern countries. Negotiable fate is a cultural belief common in Eastern societies that suggests that people can negotiate with fate for more control by exercising personal agency within the limits of what fate has determined. This study examined the moderating effects of neuroticism and negotiable fate on the relationship between bullying victimization and psychological distress among Chinese adolescents. Moreover, human society commonly suffers from multiple disasters that lead to severe mental health problems. There are few empirical studies on the effects of bullying among adolescents in multiple disaster contexts. This study included participants who experienced floods and COVID-19 simultaneously in 2021.Materials and methodsWe conducted a cross-sectional cluster sampling study from August 6 to 9, 2021, approximately 2 weeks after the start of the Zhengzhou City flooding and 7 days after the new wave of the COVID-19 outbreak in Zhengzhou City. The study included 1,207 participants (52.4% men,n = 633;Mage = 14.36,SD = 0.94) from a middle school in Zhengzhou City, China.ResultsThe results revealed that bullying was positively linked to psychological distress (β = 0.5.34,p &lt; 0.001, [0.73, 9.95]). Neuroticism and negotiable fate significantly moderated the relationship between the effects of bullying and psychological distress (β = −3.58,p &lt; 0.05, 95% CI [−6.12, −1.04]). Specifically, high neuroticism increased the risk of psychological distress in adolescents bullied before a disaster. High or low neuroticism and high negotiable fate buffered the link between bullying and psychological distress.ConclusionThis study showed that neuroticism and negotiable fate moderated the relationship between bullying victimization and psychological distress in Chinese students with COVID-19 and flood disasters. High negotiable fate and high or low neuroticism could help adolescents bullied in school to be immune from psychological distress in catastrophe. The results highlight the importance of considering the interplay between bullying, neuroticism, and a sense of controllable destiny when examining adolescents’ psychological distress.
Development of well-being after moving to telework: A longitudinal latent class analysis
Authors: Kröner Friedrich , Müller Andreas 
Published: March 2023

IntroductionDue to the COVID-19 pandemic, teleworking suddenly became a reality for many individuals. Previous research shows that there are contradictory consequences of telework on well-being: while workers have the opportunity for self-directed work, intensified work behavior as well as longer hours being worked might occur at the same time. We expect that the effects of telework vary over time and may be able to explain these contradictions. Moreover, from the perspective of the job demands-resources model besides job resources, personal resources may be relevant. The aim of this study is to investigate how the mental well-being of workers unfolds over time after the onset of the pandemic and the role of telework in this process. Additionally we seek to identify the impact of available job resources and personal resources in this extraordinary situation.MethodsData were collected online from 642 participants in Germany beginning in March 2020, with 8 weekly followup surveys. Mental well-being was measured using the WHO-5 well-being index. For personal resources we looked at occupational self-efficacy; job resources were flexible working hours, job autonomy, and social support. Job demands were telework and work intensification. First we used a group-based trajectory analysis approach to identify different well-being trajectories. Second we applied multinomial regression analysis to identify T1 predictors of well-being trajectory group membership and their interactions.ResultsWe found three groups of mental well-being trajectories: low, medium, and high. Their progress through the investigation period was rather stable: we observed only slight improvements of mental well-being for the high well-being group and a slight deterioration for the other two groups. Only the job demand work intensification and the personal resource occupational self-efficacy had a significant relationship to group assignment. Additionally we found interactions of telework with work intensification and occupational self-efficacy indicating a buffering mechanism of telework on the consequences of high work intensification; and low occupational self-efficacy.DiscussionTelework appears to be a useful resource that buffered high work intensification and compensated for low personal resources during the pandemic. Since data were from self-reports of a convenience sample we can't assume generalization of our results nor absence of common-method bias.
Individual differences in the effects of midazolam on anxiety-like behavior, learning, reward, and choice behavior in male mice
Authors: Jovita-Farias Caio , Follett Meagan E. , Dias-Junior Behaim C. , Serra Yasmim A. , Kisaki Natali D. , Barros-Santos Thaísa , de Jesus Nailton M. S. , Rodrigues Isa R. S. , Macedo Larissa E. L. , Malpezzi-Marinho Elena L. A. , Oliveira-Lima Alexandre J. , Marinho Eduardo Ary Villela , Rowlett James K. , Berro Lais F. 
Published: March 2023

IntroductionThe aim of the present study was to investigate the behavioral effects of the benzodiazepine midazolam in male mice, in models of anxiolysis, learning, and abuse-related effects.MethodsIn a first set of experiments, male Swiss mice were submitted to the training session of a discriminative avoidance (DA) task on the elevated plus maze to evaluate anxiety-like behavior and learning after vehicle or midazolam (1, 2 or 5 mg/kg, i.g.) administration. The same animals were submitted to a conditioned place preference (CPP) protocol with midazolam (1, 2 or 5 mg/kg, i.g.). In a second experiment, outbred (Swiss) and inbred (C57BL/6) male mice were submitted to a two-bottle choice (TBC) oral midazolam drinking procedure. Animals were exposed to one sucrose bottle and one midazolam (0.008, 0.016 or 0.032 mg/ml) plus sucrose bottle.ResultsMidazolam (1 and 2 mg/kg) induced anxiolytic-like effects, and all doses of midazolam prevented animals from learning to avoid the aversive closed arm during the DA training session. Assessment of midazolam reward via the CPP procedure and choice via the TBC procedure showed notable variability. A 2-step cluster analysis for the CPP data showed that midazolam data were well-fitted to 2 separate clusters (preference vs. aversion), albeit with the majority of mice showing preference (75%). Correlational and regression analyses showed no relationship between midazolam reward and anxiolytic-like effects (time spent in the open arms in the DA test) or learning/memory. Two-step cluster analysis of the TBC data also demonstrated that, regardless of strain, mice overall fell into two clusters identified as midazolam-preferring or midazolam-avoiding groups. Both midazolam preference and avoidance were concentration-dependent in a subset of mice.DiscussionOur findings show that midazolam preference is a multifactorial behavior, and is not dependent solely on the emergence of therapeutic (anxiolytic-like) effects, learning impairments, or on genetic factors (inbred vs. outbred animals).