bluuebunny/crossref_metadata_2025_split huggingface dataset:
Genetic analysis and biological characterization of H10N3 influenza A viruses isolated in China from 2014 to 2021
Authors: Zhang Yuancheng , Shi Jianzhong , Cui Pengfei , Zhang Yaping , Chen Yuan , Hou Yujie , Liu Liling , Jiang Yongping , Guan Yuntao , Chen Hualan , Kong Huihui , Deng Guohua 
Published: January 2023

AbstractThe H10 subtypes of avian influenza viruses pose a continual threat to the poultry industry and human health. The sporadic spillover of H10 subtypes viruses from poultry to humans is represented by the H10N8 human cases in 2013 and the recent H10N3 human infection in 2021. However, the genesis and characteristics of the recent reassortment H10N3 viruses have not been systemically investigated. In this study, we characterized 20 H10N3 viruses isolated in live poultry markets during routine nationwide surveillance in China from 2014 to 2021. The viruses in the recent reassortant genotype acquired their hemagglutinin (HA) and neuraminidase (NA) genes from the duck H10 viruses and H7N3 viruses, respectively, whereas the internal genes were derived from chicken H9N2 viruses as early as 2019. Receptor‐binding analysis indicated that two of the tested H10N3 viruses had a higher affinity for human‐type receptors than for avian‐type receptors, highlighting the potential risk of avian‐to‐human transmission. Animal studies showed that only viruses belonging to the recent reassortant genotype were pathogenic in mice; two tested viruses transmitted via direct contact and one virus transmitted by respiratory droplets in guinea pigs, though with limited efficiency. These findings emphasize the need for enhanced surveillance of H10N3 viruses.
The pattern of cytokines expression and dynamic changes of renal function at 6 months in patients with Omicron COVID‐19
Authors: Teng Lanbo , Song Xinyuan , Zhang Miaomiao , Han Yingying , Chang Ge , Chang Wenxiu , Shen Zhongyang 
Published: January 2023

AbstractTo analyze the dynamic changes of renal function longitudinally and investigate the cytokine profiles at 6 months in patients with Omicron COVID‐19. Forty‐seven patients with a proven diagnosis of Omicron COVID‐19 from January to February 2022 attended a 6‐month follow‐up after discharge at Tianjin First Central Hospital. The demographic parameters, clinical features, and laboratory indexes were collected during hospitalization and 6 months after discharge. The serum cytokine levels at 6 months were also assessed. Patients were grouped according to with or without kidney involvement at admission. The levels of serum creatinine and estimated glomerular filtration rate (eGFR) were all normal both in the hospital and at follow‐up. Whereas, compared with renal function in the hospital, serum creatinine levels at 6 months increased remarkably; meanwhile, eGFR decreased significantly in all patients. The serum levels of interleukin (IL)‐2, IL‐4, IL‐5, IL‐6, IL‐10, and TNF‐α and IFN‐γ significantly decreased and TGF‐β remarkably increased in the kidney involvement group. The serum levels of IL‐2 and IL‐5 were positively correlated with age; contrarily, TGF‐β showed a negative correlation with aging. The younger was an independent risk factor of the higher TGF‐β levels. Omicron patients showed a decline in renal function at follow‐up, reflecting the trend of CKD. Serum cytokine profiles were characterized with the majority of cytokines decreased and TGF‐β increased in the kidney involvement group; the latter may be used as a sign of CKD. The tendency of CKD is one of the manifestations of long COVID and deserves attention.
Upregulation of PD‐L1 by SARS‐CoV‐2 promotes immune evasion
Authors: Huang Hsiang‐Chi , Wang Shih‐Han , Fang Guo‐Chen , Chou Wen‐Cheng , Liao Chun‐Che , Sun Cheng‐Pu , Jan Jia‐Tsrong , Ma Hsiu‐Hua , Ko Hui‐Ying , Ko Yi‐An , Chiang Ming‐Tsai , Liang Jian‐Jong , Kuo Chun‐Tse , Lee Te‐An , Morales‐Scheihing Diego , Shen Chen‐Yang , Chen Shih‐Yu , McCullough Louise D. , Cui Lu , Wernig Gerlinde , Tao Mi‐Hua , Lin Yi‐Ling , Chang Yao‐Ming , Wang Shu‐Ping , Lai Yun‐Ju , Li Chia‐Wei 
Published: January 2023

AbstractPatients with severe COVID‐19 often suffer from lymphopenia, which is linked to T‐cell sequestration, cytokine storm, and mortality. However, it remains largely unknown how severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2) induces lymphopenia. Here, we studied the transcriptomic profile and epigenomic alterations involved in cytokine production by SARS‐CoV‐2‐infected cells. We adopted a reverse time‐order gene coexpression network approach to analyze time‐series RNA‐sequencing data, revealing epigenetic modifications at the late stage of viral egress. Furthermore, we identified SARS‐CoV‐2‐activated nuclear factor‐κB (NF‐κB) and interferon regulatory factor 1 (IRF1) pathways contributing to viral infection and COVID‐19 severity through epigenetic analysis of H3K4me3 chromatin immunoprecipitation sequencing. Cross‐referencing our transcriptomic and epigenomic data sets revealed that coupling NF‐κB and IRF1 pathways mediate programmed death ligand‐1 (PD‐L1) immunosuppressive programs. Interestingly, we observed higher PD‐L1 expression in Omicron‐infected cells than SARS‐CoV‐2 infected cells. Blocking PD‐L1 at an early stage of virally‐infected AAV‐hACE2 mice significantly recovered lymphocyte counts and lowered inflammatory cytokine levels. Our findings indicate that targeting the SARS‐CoV‐2‐mediated NF‐κB and IRF1‐PD‐L1 axis may represent an alternative strategy to reduce COVID‐19 severity.
Rapid detection of monkeypox virus by multiple cross displacement amplification combined with nanoparticle‐based biosensor platform
Authors: Zhou Juan , Xiao Fei , Fu Jin , Jia Nan , Huang Xiaolan , Sun Chunrong , Liu Chunyan , Huan Hui , Wang Yi 
Published: January 2023

AbstractThe current outbreak of monkeypox virus (MPXV) has become a public health emergency of international concern that highlights the need for rapid, sensitive MPXV diagnostic assays. Here, we combined isothermal multiple cross displacement amplification (MCDA) with nanoparticle‐based lateral flow biosensor (LFB) to devise a diagnostic test for the diagnosis of MPXV infection (called MPXV‐MCDA‐LFB) and differentiation of West and Central African MPXV isolates. The MPXV‐MCDA‐LFB protocol conducts isothermal MCDA reaction for DNA templates followed by LFB detection of preamplification target sequences. Two MCDA primer sets were designed targeting the D41L and ATI genes of Central and West African MPXV isolates, respectively, and the optimal condition of two MCDA reactions was 64°C for 30 min. The two MCDA reactions were decoded by LFB, which was devised for detecting three targets, including two amplicons yielded from two MCDA reactions and a chromatography control. Thus, the MPXV‐MCDA‐LFB assay could be completed within 50 min including rapid template preparation (15 min), MCDA reaction (30 min) and reporting of result (<5 min). The MPXV‐MCDA‐LFB method is very sensitive and can detect the target genes (D14L and ATI) with as low as five copies of plasmid template per reaction and 12.5 copies of pseudotyped virus in human blood samples. The MPXV‐MCDA‐LFB assay does not cross‐react with non‐MPXV templates, validating its specificity. Therefore, the MPXV‐MCDA‐LFB assay developed here is a useful tool for rapid and reliable diagnosis of MPXV infection.
Single‐cell transcriptomics dissects epithelial heterogeneity in HPV+ cervical adenocarcinoma
Authors: Wang Cong , Li Lei , Wang Fuhao , Li Xia , Sun Jujie , Li Xiaohui , Lei Tianyu , Huang Qingyu , Zhang Guangyu , Wang Hongqing , Li Dapeng , Jia Jue , Li Chunyan , Geng Feng , Yue Jinbo , Liu Chao 
Published: January 2023

AbstractThe intra‐ and intertumoral heterogeneity of epithelial cells in human papillomavirus (HPV+) cervical adenocarcinoma (CEAD) remains largely unknown. To investigate this issue, we performed single‐cell RNA sequencing on 19 229 epithelial cells sorted from three tumor samples of three patients with HPV+ CEAD. Six epithelial subclusters (Epi1–Epi6) were identified that showed distinct gene expression. Among these, Epi1 and Epi4 had apparent tumor hallmarks and metabolic activities. Epi1 was highly enriched in hallmarks of hypoxia, IL2/STAT5 signaling, retinol metabolism, glycolysis, and arachidonic acid metabolism, while Epi4 was highly enriched in hallmarks of G2M checkpoint, E2F targets, DNA repair, PI3K/AKT/MTOR signaling, glycolysis, fatty acid degradation, TCA cycle, and glutathione metabolism. We also investigated intertumoral epithelial heterogeneity and found that Patient 1 was highly enriched for KRAS signaling and angiogenesis, while Patient 2 was highly enriched for epithelial–mesenchymal transition and TGF‐β signaling, and Patient 3 was highly enriched for hypoxia, DNA repair, G2M checkpoint, and E2F targets. Using single‐cell RNA sequencing, we revealed the intra‐ and intertumoral heterogeneity of epithelial cells in HPV+ CEAD, providing insights into the importance of personalized treatment for patients with HPV+ CEAD.
Risk stratification for cervical precancer and cancer by DH3‐HPV partial genotyping and cytology in women attending cervical screening: A retrospective cohort study
Authors: Fu Yunfeng , Li Ying , Li Xiao , Wang Xinyu , Lü Weiguo 
Published: January 2023

AbstractTo evaluate the effect of DH3‐human papillomavirus (HPV) partial genotyping for risk stratification in cervical cancer screening, we conducted a post hoc analysis within a retrospective cohort of 7263 Chinese women aged 21–71 years who participated in population‐based screening. The residual cytological samples at baseline were retested with DH3‐HPV and Hybrid Capture 2 (HC2) assay after 3‐year follow‐up. Risk values with 95% confidence intervals (CIs) of cervical intraepithelial neoplasia (CIN) grade 3/2 or worse (CIN3+/CIN2+) were estimated based on HPV and cytology results. The report complies with the STROBE statement. Across every cytological result, risk estimates obtained from DH3‐HPV and HC2 were similar or almost identical. By DH3‐HPV partial genotyping, risks of CIN3+/CIN2+ were invariably higher for HPV16/18 than other high‐risk HPV (hrHPV). Among women with normal cytology, immediate CIN3+ risks were 8.16% (95% CI = 4.19%–15.28%) for HPV16/18 positive and 0.48% (95% CI = 0.13%–1.73%) for other hrHPV positive. Among women with any abnormal cytology, immediate CIN3+ risks were 33.33% (95% CI = 22.24%–46.64%) for HPV16/18, and 13.33% (95% CI = 8.37%–20.56%) for other hrHPV. Among 5840 women completed 3‐year follow‐up, the cumulative CIN3+ risk was 25.56% (95% CI = 18.91%–33.59%) for HPV16/18 and 8.22% (95% CI = 6.02%–11.13%) for other hrHPV. Women with an HPV‐negative result with DH3‐HPV or HC2 test had very low cumulative 3‐year CIN3+ risk (0.06%, 95% CI = 0.02%–0.17%), which was about one‐tenth of women with normal cytology at baseline (0.62%, 95% CI = 0.45%–0.86%). Similar patterns were observed for the endpoint of CIN2+. These findings suggest that partial genotyping of DH3‐HPV performs well in risk stratification, which can better balance the benefits and harms of cervical cancer screening.
Composite of two‐dimensional vanadium carbide MXene and cellulose nanocrystals for detection of analytes
Authors: Limaye Mukta V. , Pramanik Monidipa , Moza Anirudh , Singh Shashi B. 
Published: January 2023

AbstractA new family of two‐dimensional (2D) materials called MXenes has gained attention recently owing to their fascinating physical and chemical properties. Herein, 2D vanadium carbide (V2C) MXene was mixed with cellulose nanocrystals (CNCs) to produce an environmentally friendly V2C/CNC composite. The V2C/CNC composite exhibited surface‐enhanced Raman scattering (SERS) activity for the methylene blue (MB) dye with an enhancement factor of ~9 × 105. Here, we have proposed that along with electromagnetic enhancement, the charge transfer process plays a vital role in the SERS enhancement. We believe that this work would open a new way to fabricate sustainable and renewable MXene and CNC composite solution‐based SERS platforms for analyte detection.
Assessment of the anharmonic phonon decay, Fröhlich interactions, and phonon lifetime in Zn0.98Mn0.02S thin films through temperature‐dependent Raman spectroscopy
Authors: Ganesha Krishna V S , Mahesha M G 
Published: January 2023

AbstractThis report accounts for the occurrence of the temperature‐induced variation in the phonon properties and the spatial variation in the Raman spectra for the spray‐deposited Zn0.98Mn0.02S thin films. The study of the temperature dependence of the Raman line position, line width, and the phonon lifetime using the theoretical models that consider the thermal expansion and three‐ and four‐phonon anharmonic effects is the novelty of this work. The lifetime of the LO phonon in Zn0.98Mn0.02S thin film was as low as 0.234 ps, indicating material suitability for optical limiting applications. The intensity ratio I2LO/I1LO ranges within 0.5 at most of the (x, y) positions in the Raman mapping data, indicating the nanocrystalline nature of Zn0.98Mn0.02S.
Constitutive and conditional gene knockout mice for the study of intervertebral disc degeneration: Current status, decision considerations, and future possibilities
Authors: Lu Ze‐Yu , Chen Peng‐Bo , Xu Qing‐Yin , Li Bo , Jiang Sheng‐Dan , Jiang Lei‐Sheng , Zheng Xin‐Feng 
Published: January 2023

AbstractThere have been an increasing number of patients with degenerative disc diseases due to the aging population. In light of this, studies on the pathogenesis of intervertebral disc degeneration have become a hot topic, and gene knockout mice have become a valuable tool in this field of research. With the development of science and technology, constitutive gene knockout mice can be constructed using homologous recombination, zinc finger nuclease, transcription activator‐like effector nuclease technology and clustered regularly interspaced short palindromic repeats/Cas9 (CRISPR/Cas9) system, and conditional gene knockout mice can be constructed using the Cre/LoxP system. The gene‐edited mice using these techniques have been widely used in the studies on disc degeneration. This paper reviews the development process and principles of these technologies, functions of the edited genes in disc degeneration, advantages, and disadvantages of different methods and possible targets of the specific Cre recombinase in intervertebral discs. Recommendations for the choice of suitable gene‐edited model mice are presented. At the same time, possible technological improvements in the future are also discussed.
Building heritage brand equity through social media sales promotion: The role of Power Distance
Authors: Crespo‐Almendros Esmeralda , Prados‐Peña Ma Belén , Porcu Lucia , Alcántara Pilar Juan Miguel 
Published: January 2023

AbstractThis paper aims to look into the moderating effect of individual's cultural characteristics—Power Distance—on the relationship between the use of discounts and gifts and the formation of the brand equity of a heritage tourist site is assessed. The results of a quasi‐experimental 2×2 factorial design among Internet users from two countries and using two different promotional stimuli showed: a positive effect of sales promotions on social media on the formation of brand equity of a heritage tourist site; a lesser effect of online price discounts than free gifts; the moderating role of Power Distance on the formation of brand equity.
Manipulation of Scattering Spectra with Topology of Light and Matter
Authors: Barati Sedeh Hooman , Pires Danilo G. , Chandra Nitish , Gao Jiannan , Tsvetkov Dmitrii , Terekhov Pavel , Kravchenko Ivan , Litchinitser Natalia 
Published: January 2023

AbstractStructured lights, including beams carrying spin and orbital angular momenta, radially and azimuthally polarized vector beams, as well as spatiotemporal optical vortices, have attracted significant interest due to their unique amplitude, phase front, polarization, and temporal structures, enabling a variety of applications in optical and quantum communications, micromanipulation, and super‐resolution imaging. In parallel, structured optical materials, metamaterials, and metasurfaces consisting of engineered unit cells—meta‐atoms, opened new avenues for manipulating the flow of light and optical sensing. While several studies explored structured light effects on the individual meta‐atoms, their shapes are largely limited to simple spherical geometries. However, the synergy of the structured light and complex‐shaped meta‐atoms has not been fully explored. In this paper, the role of the helical wavefront of Laguerre–Gaussian beams in the excitation and suppression of higher‐order resonant modes inside all‐dielectric meta‐atoms of various shapes, aspect ratios, and orientations, is demonstrated and the excitation of various multipolar moments that are not accessible via unstructured light illumination is predicted. The presented study elucidates the role of the complex phase distribution of the incident light in shape‐dependent resonant scattering, which is of utmost importance in a wide spectrum of applications ranging from remote sensing to spectroscopy.
Meta‐Structured Silicon Nanophotonic Polarization Beam Splitter with an Optical Bandwidth of 415 nm
Authors: Xu Hongnan , Qin Yue , Hu Gaolei , Tsang Hon Ki 
Published: January 2023

AbstractThe polarization beam splitter (PBS) is a pivotal element in the polarization management of free‐space optical instruments and systems. Photonic integrated circuits for sensing, imaging, communications, and quantum‐information processing also have needs for monolithically integrated PBSs with an ultra‐broad optical bandwidth. In this paper, a novel silicon nanophotonic PBS inspired by the crystalline Glan–Thompson prism but implemented with silicon subwavelength‐grating (SWG) metamaterials is presented. Due to the tailored artificial anisotropy of SWGs, the meta‐prism functions like a thin‐film reflector or a waveguide crossing for different polarizations. Thus, the incident light can be steered with strong polarization selectivity and negligible wavelength dependence. Unlike conventional PBS designs, the routing of polarized light is enabled by the wavelength‐independent total internal reflection in anisotropy‐engineered effective media, thereby breaking the bandwidth limit. The device footprint is as small as ≈15 × 7 µm2. Low insertion losses of 0.6–1.7 dB and high extinction ratios of 20–30 dB are experimentally achieved spanning a record broad bandwidth of over 415 nm, ranging from 1.26 to 1.675 µm wavelength. These results represent, to the best of their knowledge, the most broadband integrated PBS ever demonstrated to date.
Cr3+‐Facilitated Ultra‐Sensitive Luminescence Ratiometric Thermometry at Cryogenic Temperature
Authors: Li Rui , Wei Guohui , Wang Zhijun , Wang Ye , Li Jiehong , He Shaoxuan , Li Leipeng , Suo Hao , Ding Wenge , Li Panlai 
Published: January 2023

AbstractNon‐contact ratiometric luminescence thermometry continues to gain popularity over decades due to its fascinating features of high accuracy, fast response, and simplicity. In this regard, rational design of optical thermometers with ultrahigh relative sensitivity and low temperature resolution has become a cutting‐edge topic. Herein, a luminescence intensity ratio thermometric method depending on the emission line at 698 nm (peak 1) and the emission bands at 713 and 721 nm (peaks 2 and 3) of Li1.97Zn1.0292Ge3O8:0.08%Cr3+ is proposed. As temperature elevates from 50 to 300 K, the 698 nm emission line undergoes an 84‐fold increase while the 713 and 721 nm emission bands show the opposite decline trend. This fascinating feature enables high relative sensitivity of 13.09% K−1 and low temperature resolution of 0.032 K, which are superior to most of the reported optical thermometers. This work is expected to provide more insights for designing excellent and reliable optical thermometers by employing Cr3+ activated luminescent materials.
Manipulating Crystallization Dynamics for Efficient and Spectrally Stable Blue Perovskite Light‐Emitting Diodes
Authors: Cao Longxue , Shen Yang , Lei Bing , Zhou Wei , Li Shuhong , Liu Yunlong , Lu Yu , Zhang Kai , Ren Hao , Li Yan‐Qing , Tang Jian‐Xin , Wang Wenjun 
Published: January 2023

AbstractReduced‐dimensional perovskite light‐emitting diodes (PeLEDs) have shown great potential in solution‐processed high‐definition displays. However, the inferior electroluminescent (EL) performance of blue PeLEDs has become a huge challenge for their commercialization. The inefficient domain control [number of PbX6− layers (n)] and deleterious phase segregation make the blue PeLEDs suffer from low EL efficiency and poor spectral stability. Here, a rational strategy for perovskite crystallization control by adjusting the precursor concentration is proposed for improving phase distribution and suppressing ion migration in reduced‐dimensional mixed‐halide blue perovskite films. Based on this method, efficient sky‐blue PeLEDs exhibit a maximum external quantum efficiency (EQE) of 8.5% with stable EL spectra at 482 nm. Additionally, spectrally stable pure‐blue PeLEDs at 474 and 468 nm are further obtained with maximum EQEs of 4.0% and 2.4%, respectively. These findings may provide an alternative scheme for manipulating perovskite crystallization dynamics toward efficient and stable PeLEDs.
Zwitterionic Polysulfobetaine Coating and Antiplatelet Liposomes Reduce Fouling in Artificial Lung Circuits
Authors: Amoako Kagya , Kaufman Rikki , Haddad Waad A. M. , Pusey Romario , Saniesetty Venkata H. K. , Sun Hao , Skoog David , Cook Keith 
Published: January 2023

AbstractThe artificial lung has provided life‐saving support for pulmonary disease patients and recently afforded patients with severe cases of COVID‐19 better prognostic outcomes. While it addresses a critical medical need, reducing the risk of clotting inside the device remains challenging. Herein, a two‐step surface coating process of the lung circuit using Zwitterionic polysulfobetaine methacrylate is evaluated for its nonspecific protein antifouling activity. It is hypothesized that similarly applied coatings on materials integrated (IT) or nonintegrated (NIT) into the circuit will yield similar antifouling activity. The effects of human plasma preconditioned with nitric oxide‐loaded liposome on platelet (plt) fouling are also evaluated. Fibrinogen antifouling activities in coated fibers are similar in the IT and NIT groups. It however decreases in coated polycarbonate (PC) in the IT group. Also, plt antifouling activity in coated fibers is similar in the IT and NIT groups and is lower in coated PC and Tygon in the IT group compared to the NIT group. Coating process optimization in the IT lung circuit may help address difference in the coating appearance of outer and inner fiber bundle fibers, and the NO‐liposome significantly reduces (86%) plt fouling on fibers indicating its potential use for blood anticoagulation.