bluuebunny/crossref_metadata_2025_split huggingface dataset:
Forced-air prewarming prevents hypothermia during living donor liver transplantation: a randomized controlled trial
Authors: Oh Eun Jung , Han Sangbin , Lee Sooyeon , Choi Eun Ah , Ko Justin S. , Gwak Mi Sook , Kim Gaab Soo 
Published: April 2023

AbstractDespite various intraoperative thermal strategies, core heat loss is considerable during liver transplantation and hypothermia is common. We tested whether forced-air prewarming prevents hypothermia during liver transplantation. Adult patients undergoing living donor liver transplantation were randomly assigned to non-prewarming group (n = 20) or prewarming group (n = 20). Patients in prewarming group underwent 30-min forced-air warming before anesthetic induction. During surgery, core temperature was measured in the pulmonary artery. The primary outcome was intraoperative hypothermia (< 36.0 °C). The secondary outcomes included plasma lactate concentration. Intraoperative hypothermia risk was significantly lower in prewarming group than in non-prewarming group (60.0% vs. 95.0%, P = 0.020). The difference in hypothermia incidence between groups was greater in the post-induction phase (20.0% vs. 85.0%, P < 0.001) than in the anhepatic or post-reperfusion phase, suggesting that prewarming mainly acts on preventing post-induction core-to-peripheral heat redistribution. Hypothermia duration was significantly shorter in prewarming group (60 [0–221] min vs. 383 [108–426] min, P = 0.001). Lactate concentration decreased during 3 h after graft reperfusion in prewarming group, whereas it continuously increased in non-prewarming group (− 0.19 [− 0.48 to 0.13] mmol/L vs. 1.17 [3.31–0.77] mmol/L, P = 0.034). In conclusion, forced-air prewarming decreases the incidence and duration of intraoperative hypothermia with potential clinical benefit while mainly acting by preventing the core-to-peripheral heat redistribution.Clinical trial registration: Registered at the Clinical Research Information Service (https://cris.nih.go.kr, [KCT0003230]) on 01/10/2018.
Prognostic factors and Doxorubicin involved in malignant progression of meningioma
Authors: Huo Xulei , Song Lairong , Wang Ke , Wang Hongyi , Li Da , Li Huan , Wang Wei , Wang Yali , Chen Lei , Zhao Zongmao , Wang Liang , Wu Zhen 
Published: April 2023

AbstractMeningioma was the most primary intracranial tumor, but the molecular characteristics and the treatment of malignant meningioma were still unclear. Nine malignant progression-related genes based prognostic signatures were identified by transcriptome analysis between benign meningioma and malignant meningioma. The external dataset GEO136661 and quantitative Real-time Polymerase Chain Reaction were used to verify the prognostic factors. has-miR-3605-5p, hsa-miR-664b-5p, PNRC2, BTBD8, EXTL2, SLFN13, DGKD, NSD2, and BVES were closed with malignant progression. Moreover, Doxorubicin was identified by Connectivity Map website with the differential malignant progression-related genes. CCK-8 assay, Edu assay, wound healing assay, and trans-well experiment were used to reveal that Doxorubicin could inhibit proliferation, migration and invasion of IOMM-Lee Cells.
Assessment of the tocolytic nifedipine in preclinical primary models of preterm birth
Authors: Arman Bridget M. , Binder Natalie K. , de Alwis Natasha , Beard Sally , Debruin Danielle A. , Hayes Alan , Tong Stephen , Kaitu’u-Lino Tu’uhevaha J. , Hannan Natalie J. 
Published: April 2023

AbstractSpontaneous preterm birth is the leading cause of perinatal morbidity and mortality. Tocolytics are drugs used in cases of imminent preterm birth to inhibit uterine contractions. Nifedipine is a calcium channel blocking agent used to delay threatened spontaneous preterm birth, however, has limited efficacy and lacks preclinical data regarding mechanisms of action. It is unknown if nifedipine affects the pro-inflammatory environment associated with preterm labour pathophysiology and we hypothesise nifedipine only targets myometrial contraction rather than also mitigating inflammation. We assessed anti-inflammatory and anti-contractile effects of nifedipine on human myometrium using in vitro and ex vivo techniques, and a mouse model of preterm birth. We show that nifedipine treatment inhibited contractions in myometrial in vitro contraction assays (P = 0.004 vs. vehicle control) and potently blocked spontaneous and oxytocin-induced contractions in ex vivo myometrial tissue in muscle myography studies (P = 0.01 vs. baseline). Nifedipine treatment did not reduce gene expression or protein secretion of pro-inflammatory cytokines in either cultured myometrial cells or ex vivo tissues. Although nifedipine could delay preterm birth in some mice, this was not consistent in all dams and was overall not statistically significant. Our data suggests nifedipine does not modulate preterm birth via inflammatory pathways in the myometrium, and this may account for its limited clinical efficacy.
Estimation of the depth of origin of fluids using noble gases in the surface sediments of submarine mud volcanoes off Tanegashima Island
Authors: Mitsutome Yuki , Toki Tomohiro , Kagoshima Takanori , Sano Yuji , Tomonaga Yama , Ijiri Akira 
Published: April 2023

AbstractThe helium isotope ratio (3He/4He), concentration ratio of neon-20 to helium-4 (20Ne/4He), argon (Ar), krypton (Kr), and xenon (Xe) concentrations were measured in the porewater of surface sediments of several submarine mud volcanoes. From the 3He/4He values (0.18–0.93RA), the estimated He origin is almost 90% crustal He, with little contribution from mantle-derived He. The determined Ar, Kr, and Xe concentrations lie within the solubility equilibrium range expected for temperatures from 83 °C up to 230 °C and are consistent with the temperature range of the dehydration origin of clay minerals. Considering the geothermal gradient in the investigated region (25 °C/km), these gases are considered to have reached dissolution equilibrium at a depth of about 3.3 km to 9.2 km below the seafloor. As the depth of the plate boundary is 18 km below the seafloor, the noble gas signatures are likely to originate from the crust, not from the plate boundary. This is consistent with the results presented by the He isotope ratios.
Graph-BERT and language model-based framework for protein–protein interaction identification
Authors: Jha Kanchan , Karmakar Sourav , Saha Sriparna 
Published: April 2023

AbstractIdentification of protein–protein interactions (PPI) is among the critical problems in the domain of bioinformatics. Previous studies have utilized different AI-based models for PPI classification with advances in artificial intelligence (AI) techniques. The input to these models is the features extracted from different sources of protein information, mainly sequence-derived features. In this work, we present an AI-based PPI identification model utilizing a PPI network and protein sequences. The PPI network is represented as a graph where each node is a protein pair, and an edge is defined between two nodes if there exists a common protein between these nodes. Each node in a graph has a feature vector. In this work, we have used the language model to extract feature vectors directly from protein sequences. The feature vectors for protein in pairs are concatenated and used as a node feature vector of a PPI network graph. Finally, we have used the Graph-BERT model to encode the PPI network graph with sequence-based features and learn the hidden representation of the feature vector for each node. The next step involves feeding the learned representations of nodes to the fully connected layer, the output of which is fed into the softmax layer to classify the protein interactions. To assess the efficacy of the proposed PPI model, we have performed experiments on several PPI datasets. The experimental results demonstrate that the proposed approach surpasses the existing PPI works and designed baselines in classifying PPI.
A temporal single cell transcriptome atlas of zebrafish anterior segment development
Authors: Vöcking Oliver , Famulski J. K. 
Published: April 2023

AbstractAnterior segment dysgenesis (ASD), resulting in vision impairment, stems from maldevelopment of anterior segment (AS) tissues. Incidence of ASD has been linked to malfunction of periocular mesenchyme cells (POM). POM cells specify into anterior segment mesenchyme (ASM) cells which colonize and produce AS tissues. In this study we uncover ASM developmental trajectories associated with formation of the AS. Using a transgenic line of zebrafish that fluorescently labels the ASM throughout development, Tg[foxc1b:GFP], we isolated GFP+ ASM cells at several developmental timepoints (48–144 hpf) and performed single cell RNA sequencing. Clustering analysis indicates subdifferentiation of ASM as early as 48 hpf and subsequent diversification into corneal epithelium/endothelium/stroma, or annular ligament (AL) lineages. Tracking individual clusters reveals common developmental pathways, up to 72 hpf, for the AL and corneal endothelium/stroma and distinct pathways for corneal epithelium starting at 48 hpf. Spatiotemporal validation of over 80 genes found associated with AS development demonstrates a high degree of conservation with mammalian trabecular meshwork and corneal tissues. In addition, we characterize thirteen novel genes associated with annular ligament and seven with corneal development. Overall, the data provide a molecular verification of the long-standing hypothesis that POM derived ASM give rise to AS tissues and highlight the high degree of conservation between zebrafish and mammals.
Investigation of the body-centred tetragonal structure of Fe–Co–V–N Bulk foils using the rolling and ammonia-gas-nitriding method
Authors: Hasegawa Takashi 
Published: April 2023

AbstractImproving the properties of permanent magnets is essential for the advancement of electric motors in reducing energy consumption and carbon emissions. This study investigated the effect of V and N addition to FeCo foils on the stability of tetragonally distorted FeCo-based bulk magnets. The incorporation of these two elements stabilised the body-centred tetragonal structure in thin-film and bulk systems. Fe–Co–V ingots were rolled and nitrided by ammonia gas at 650 °C for 5 h. A body-centred tetragonal lattice with an axial ratio of c/a ≈ 1.1 was observed by transmission electron microscopy, and the collected data suggested the induction of a large uniaxial magnetic anisotropy. This study is expected to improve our understanding of rare-earth-free magnets.
Self-isolated MIMO antenna using mixed coupling by close coupling technique
Authors: Singh Harsh Verdhan , Prasad D. Venkata Siva , Tripathi Shrivishal 
Published: April 2023

AbstractA self-isolated multiple-input-multiple-output (MIMO) antenna in a compact shared ground structure is proposed for 5G systems. The proposed MIMO antenna consists of customized M-pattern, closely coupled members. It benefits to attain good isolation of the targeted bandwidth transversely without additional de-coupling structures. It is discovered that the arm of M-pattern antenna members can cancel out the coupling on the system and achieve sound isolation among antenna members. A relevant matching circuit model is discussed to show how the suggested theory works in principle. The mixed couplings among antenna members neutralize by modifying the antenna shape with the help of electric and magnetic coupling and surface currents. The proposed self-isolated 2-member MIMO antenna demonstrates sound isolation superior to 15 dB transversely in the frequency bands dedicated to 5G NR: n48/n78 and long-term evolution (LTE) band 42/43/48/52 (3.2–3.98 GHz). Moreover, the proposed 2-member design structure has a scalability advantage. It is extended into an 8-members structure, where a pair of antennas located at each side of the frame offers orthogonality. The proposed 8-members M-pattern MIMO is validated using fabricated and simulated measurements. The investigational results show that the 8-members MIMO (M-shaped) system is effective in higher order MIMO antenna design and offers more than 20 dB isolation transversely in the frequency band 5G NR n48 and LTE band 42/43/52(3.29–3.66 GHz).
Effects of left anodal transcranial direct current stimulation on hypothalamic–pituitary–adrenal axis activity in depression: a randomized controlled pilot trial
Authors: Pedraz-Petrozzi Bruno , Sardinha Helena , Gilles Maria , Deuschle Michael 
Published: April 2023

AbstractThe main objective of this study was to evaluate the effect of left anodal transcranial direct current stimulation (tDCS) on hypothalamic–pituitary–adrenal axis (HPAA) activity in individuals with depression. We conducted a 3-week, randomized, triple-blind pilot trial with 47 participants (dropout rate: 14.89%) randomly assigned to either the tDCS or control group (sham stimulation). Salivary cortisol was used as an HPAA activity marker since cortisol is the effector hormone of the HPAA. The primary outcome was the effect of tDCS on the diurnal cortisol pattern (DCP and area under the curve with respect to ground -AUCg-). Secondary outcomes included tDCS effects on cortisol awakening response (CAR) and cortisol decline (CD), as well as the variation of cortisol concentrations between the initiation of tDCS and 2 weeks later. Intention-to-treat and per-protocol analyses were conducted. Our primary outcome showed an absent effect of tDCS on DCP and AUCg. Additionally, tDCS had an absent effect on CAR, CD, and cortisol concentration variation before-after stimulation. Our pilot study suggests that anodal tDCS showed an absent effect on HPAA activity in individuals with depression. More studies are needed to confirm these findings.
Monte Carlo simulations of synchrotron X-ray dose affecting root growth during in vivo tomographic imaging
Authors: Moraes Isabela C. , Hesterberg Dean , Bacchim Neto Fernando A. , Archilha Nathaly L. , Pérez Carlos A. , Araújo Maria Victória A. , Ferreira Talita R. 
Published: April 2023

AbstractSynchrotron X-ray computed tomography (XCT) has been increasingly applied to study the in vivo dynamics of root growth and rhizosphere processes. However, minimizing radiation-induced damage to root growth warrants further investigation. Our objective was to develop a robust approach for modeling and evaluating ways to reduce synchrotron X-ray dose effects on root growth during in vivo imaging. Wheat roots growing in soil were exposed to X-rays during XCT experiments resolved in space (3D) plus time (4D). The dose rate and cumulative absorbed dose in roots were modelled using the Monte Carlo code FLUKA for different experimental conditions of polychromatic and quasi-monochromatic X-ray beam configurations. The most impactful factors affecting damage to roots were incident X-ray energy spectrum, stored current in the accelerator machine, position of the root in the soil, and possibly the number of exposures during the 4D XCT experiments. Our results imply that radiation dose during in vivo imaging of plant roots can be diminished by using monochromatic radiation at the highest energy suitable for a given sample thickness and field of view, and by controlling the rotation axis of off-centered roots to increase attenuation of radiation by the soil matrix.
Ketorolac modulates Rac-1/HIF-1α/DDX3/β-catenin signalling via a tumor suppressor prostate apoptosis response-4 (Par-4) in renal cell carcinoma
Authors: Sonawane Vinay , Ghosalkar Jeevan , Achrekar Swati , Joshi Kalpana 
Published: April 2023

AbstractRenal cell carcinoma (RCC) is the most difficult-to-treat form of kidney cancer with a median 5-year survival of 10% under metastatic setting. In RCC, although cytoreductive nephrectomy is common, approximately 20–30% of patients will develop recurrent cancer after surgery, which highlights the need for an effective therapy. Rho-GTPases viz, Rac-1 and Cdc42 are the central regulators of cancer cell migration and invasion and thus metastasis in multiple cancer types. Hence, we elucidated the role of Ketorolac, a modulator Rho-GTPases against RCC through potentiation of tumor suppressor Par-4. The effect of Ketorolac alone and in combination on proliferation, apoptosis, cell-cycle progression, migration, tumor inhibition and their related markers were studied. Moreover, Ketorolac’s impact on metastasis by influencing Rac-1/HIF-1α/DDX3/β-catenin signalling was studied with respect to its ability to modulate the expression of tumor suppressor Par-4, and this mechanism was confirmed by siRNA knockdown studies. Ketorolac induced cytotoxicity in a panel of renal cells including patient derived tumor cells with IC50 2.8 to 9.02 mM and 0.28 to 3.8 mM in monolayer and anchorage independent clonogenic assays respectively. Ketorolac caused significant down regulation of proliferation (Ki-67, Cyclin D1, pRB and DDX3), migration/invasion (Rac-1, Cdc42, and Tiam1), and angiogenesis (HIF-1α and VEGF) markers as studied by gene and protein expression. Moreover, it caused a significant upregulation of tumor suppressor Par-4 known to be downregulated in RCC. This mechanism was further confirmed by using siRNA knockdown studies where we could demonstrate a negative relation between the expression of Par-4 and Rac-1/Cdc42. Importantly, Ketorolac alone and in combination with Sunitinib showed tumor growth inhibition (TGI) of 73% and 86% respectively in xenograft model. This anti-tumor activity was further corroborated by down regulation of Rac-1/Cdc42/HIF-1α/DDX3/β-catenin signalling. This is the first report which implicates the role of Ketorolac against RCC by acting as a small molecule secretagogue causing upregulation of Par-4 in autocrine and paracrine manner. Consequently, these findings suggest that Par-4 can serve as a valuable therapeutic target and a prognostic marker for the treatment of RCC.
Rational design of phase separating peptides based on phase separating protein sequence of p53
Authors: Kamagata Kiyoto , Hando Atsumi , Ariefai Maulana , Iwaki Nanako , Kanbayashi Saori , Koike Ryotaro , Ikeda Keisuke 
Published: April 2023

AbstractArtificial phase-separating (PS) peptides can be used in various applications such as microreactors and drug delivery; however, the design of artificial PS peptides remains a challenge. This can be attributed to the limitation of PS-relevant residues that drive phase separation by interactions of their pairs in short peptides and the difficulty in the design involving interaction with target PS proteins. In this study, we propose a rational method to design artificial PS peptides that satisfy the requirements of liquid droplet formation and co-phase separation with target PS proteins based on the target PS protein sequence. As a proof of concept, we designed five artificial peptides from the model PS protein p53 using this method and confirmed their PS properties using differential interference contrast and fluorescence microscopy. Single-molecule fluorescent tracking demonstrated rapid diffusion of the designed peptides in their droplets compared to that of p53 in p53 droplets. In addition, size-dependent uptake of p53 oligomers was observed in the designed peptide droplets. Large oligomers were excluded from the droplet voids and localized on the droplet surface. The uptake of high-order p53 oligomers into the droplets was enhanced by the elongated linker of the designed peptides. Furthermore, we found that the designed peptide droplets recruited p53 to suppress gel-like aggregate formation. Finally, we discuss aspects that were crucial in the successful design of the artificial PS peptides.
Context expectation influences the gait pattern biomechanics
Authors: Ciceri Tommaso , Malerba Giorgia , Gatti Alice , Diella Eleonora , Peruzzo Denis , Biffi Emilia , Casartelli Luca 
Published: April 2023

AbstractBeyond classical aspects related to locomotion (biomechanics), it has been hypothesized that walking pattern is influenced by a combination of distinct computations including online sensory/perceptual sampling and the processing of expectations (neuromechanics). Here, we aimed to explore the potential impact of contrasting scenarios (“risky and potentially dangerous” scenario; “safe and comfortable” scenario) on walking pattern in a group of healthy young adults. Firstly, and consistently with previous literature, we confirmed that the scenario influences gait pattern when it is recalled concurrently to participants’ walking activity (motor interference). More intriguingly, our main result showed that participants’ gait pattern is also influenced by the contextual scenario when it is evoked only before the start of walking activity (motor expectation). This condition was designed to test the impact of expectations (risky scenario vs. safe scenario) on gait pattern, and the stimulation that preceded walking activity served as prior. Noteworthy, we combined statistical and machine learning (Support-Vector Machine classifier) approaches to stratify distinct levels of analyses that explored the multi-facets architecture of walking. In a nutshell, our combined statistical and machine learning analyses converge in suggesting that walking before steps is not just a paradox.
Drug-independent control strategy of clostridial infection in broiler chickens using anti-toxin environmentally friendly multienzymes
Authors: Saleh Ahmed A. , Hafez Abdelhaleem , Amber Khairy , Abdelhady AbdelRahman Y. , Salem Heba M. , Fathy M. , Kamal M. A. , Alagawany Mahmoud , Alzawqari Mohammed H. 
Published: April 2023

AbstractThe study investigated the effect of enzymes as a toxin detoxifier (DETOXIZYME) dietary supplementation on performance during growth, blood chemistry, and immunity under clostridia infection in chickens. A total of 480, day-old male chicks were randomly distributed to four groups, with six replicates of 20 birds each. The first control negative treatment (A) fed the basal formula as commercial feed prepared following the strain's needs, the second control positive group (B) fed the basal formula challenged with Clostridium perfringens (C. perfringens) type A, the third group (C) fed the basal formula with 100 g DETOXIZYME/ton of feed and challenged with clostridia, and the fourth group (D) fed the control basal formula with 100 g DETOXIZYME/ton of feed. DETOXIZYME dietary supplementation significantly boosted body weight (BW), body weight gain (BWG), feed intake (FI), and European production efficiency factor (EPEF) and improved the feed conversion rate (FCR) of the broilers. The dietary supplementation of DETOXIZYME significantly increased carcass trait and spleen. However, liver and abdominal fat weight significantly decreased compared with clostridia-challenged groups. The values of alanine aminotransferase (ALT), aspartate aminotransferase (AST), uric acid, creatinine, and Malondialdehyde (MDA) were decreased. While calcium, phosphate, zinc, and glutathione peroxidase (GPx) levels were improved in birds that took basal formulas fortified with DETOXIZYME contrary to the other treatment groups during 35 days of age. Plasma total cholesterol, triglyceride, and low-density lipoprotein (LDL) values were reduced versus the other treatment groups. Dietary supplementation of DETOXIZYME increased total protein, albumin, globulin, and Newcastle Disease (ND) immunity titer levels in the overall period compared to other groups. Dietary DETOXIZYME supplementation decreased clostridia and E. coli bacteria counts and improved gut morphometry. In conclusion, dietary supplementation of DETOXIZYME had a positive impact on performance, blood biochemistry, immunity, and bacterial counts and improved the gut morphology in broilers under clostridia infection.