bluuebunny/crossref_metadata_2025_split huggingface dataset:
The Effect of Using Bamboo Dancing Cooperative Learning Strategy Towards Student English Speaking Skill in Senior High School 3 Jambi City
Authors: Gita Yozi Sonor , Ismiyati Yanti 
Published: May 2023

The purpose of this study was to find out the effect or not there was any effect of using Bamboo Dancing Cooperative Learning towards Student English Speaking Skill. The problem in this study referred to the learning strategy English speaking skills. The study was conducted at the MIPA 6 and MIPA 7 in senior high school. The population of the study was 71 student in the second semester of MIPA 6 and MIPA 7. The sample of this study was all of the 36 is MIPA 6, 22 female and 14 male students. The types of the data were quantitative. The data were obtained by using tell the recount text from the picture to test the speaking of students, and analyzed by using manual effect analysis with speaking table score, oral proficiency scoring categories. Furthermore based on statistical compulation t-test was found that the coefficient of = 4,05 where the coefficient of = 1,994. It means that there significant effect of using bamboo dancing method toward students’ english speakig skill. It also indicated that there significant effect of using Bamboo Dancing Cooperative Strategy Towards Students English Speaking Skill in Senior High School 3 Jambi City in 2020-2021 Academic Year.Keywords: Speaking, Bamboo Dancing, Cooperative Learning Strategy
Comparison of Ketamine and Fentanyl with Propofol for Procedural Sedation and Analgesia for Dilatation and Curettage
Authors: Ershad Reza , Hossain Md Mozaffor , Maruf Abdullah Al , Nazrina Sayeda 
Published: May 2023

Introduction: Dilatation and curettage (D&C) is a common procedure that generally causes considerable pain and usually done under procedural sedation and analgesia. Propofol is an ideal intravenous anaesthtic agent for short interventional procedure like D&C but lack of analgesia remains it’s main shortcoming therefore it is always combined with an analgesic. Ketamine and fentanyl are the popular analgesic in this context.
Objectives: This prospective clinical study was designed to evaluate to compare propofol ketamine combination versus propofol fentanyl combination in respect of hemodynamics and recovery time for procedural sedation and analgesia in patients undergoing D&C.
Methods: This prospective randomized study was performed on 100 patients who underwent elective D&C procedure. Patients were randomly allocated into two groups of fifty each: group PK received propofol 2mg/kg + ketamine 1mg/kg for induction and propofol 4mg/kg/hr + ketamine 1 mg/kg/hr for maintenance anesthesia, group PF received propofol 2 mg/kg + fentanyl 2 ìg/kg for induction and propofol 4 mg/kg/hr + fentanyl 1ìg/kg/hr for maintenance of anesthesia. The pulse rate, systolic and diastolic arterial blood pressures and peripheral oxygen saturation were recorded. Recovery times, side effects of sedation were also recorded.
Results: Demographic data were found similar in two groups. There were no significant differences in heart rate, systolic and diastolic arterial blood pressure in all time intervals among groups except there was statistically significant fall in systolic blood pressure after induction in PF group (P=0.005). Recovery time was statistically significant increase in Group PK compared to Group PF (p=0.004). There were no significant differences among groups in regard to side effects.
Conclusion: Propofol ketamine and propofol fentanyl had similar hemodynamic stability without any important side effects for procedural sedation and analgesia in patients underwent D&C but propofol ketamine had longer recovery time.
JBSA 2017; 30(1): 14-20
PELATIHAN METODE IDENTIFIKASI MOTORIK SISWA MENGGUNAKAN TES TGMD-2 DI DESA JAYALAKSANA
Authors: Sulaeman Trio Wibowo , Ekowati Ekowati , Haqiyah Aridhotul 
Published: May 2023

The TGMD-2 test is one of the motion development tests developed by Ulirch in 1985. Ulrich D. A. from the School of kinesiology at the University of Michigan, has compiled a test to evaluate 250 basic motor skills (Test Of Gross Motor Development). TGMD-2 tests 12 basic movement skills which are divided into two subtests, namely locomotor and object control. This test is designed to assess the level of basic motor skills in children aged 3-10 years. This test is suitable for elementary school age students 7-10 years. In community service this time using the training method in the form of demonstrations related to the rules, movements and procedures for carrying out the TGMD-2 Test. The main target for this program is sports teachers who work at the Jayalaksana Village Elementary School, Kec. CabangBungin, Bekasi Regency.The purpose of implementing this program is to improve motor understanding of sports teachers through training in methods of identifying motor skills of elementary school students through the TGMD-2 test. The results obtained showed that the teacher representatives from each elementary school in Jayalaksana Village were able to provide good understanding and direction of the TGMD-2 test movements to 6 (six) students who were appointed as demonstrators in the demonstration of the TGMD-2 test movement. So it can be concluded that this Community service activity has an impact, namely being able to increase knowledge and information related to the material and implementation of the TGMD-2 Test for teachers who teach sports, as well as provide knowledge about the importance of motor development for elementary school students in Jayalaksana Village because they have It is the duty and responsibility of a teacher to know the progress of his students.

Keyword:Motor Skills, TGMD-2 Test, Sports Teachers
Bioactive profiling and evaluation of anti-proliferative and anti-cancerous properties of Shivagutika, an Indian polyherbal formulation synchronizing in vitro and in silico approaches
Authors: V. H. Pushpa , Kuruburu Mahadevaswamy G. , M. K. Jayanthi , N. Akshaya Simha , Taha Babakr Abdullatif , Sreenivasan Rajesh , Ramu Ramith , Madhunapantula SubbaRao V. 
Published: May 2023

Shivagutika is a polyherbal formulation mentioned in Ayurveda, the oldest system of medicine. The aim of this study was to investigate the anti-breast cancer potential of DCM extract of Shivagutika using MCF-7, MDA-MB-231, and MDA-MB-468. Primarily, various extracts of Shivagutika were prepared and subjected to primary in vitro analysis—total protein, phenolic acid content, and flavonoid content. DCM extract among all the extracts showed the promising results hence, it was subjected to LC-MS/MS analysis to identify the phytochemicals. The same extract was subjected to anti-proliferation assay and anti-cancer assay. It inhibited all the 3 cell lines and increased the activity of Caspase 3, pro-apoptotic protein. Further, to find the potent molecule(s) in silico analysis (molecular docking and molecular dynamics simulation studies) was performed. Sciadopitysin was identified as a potent molecule among all phytochemicals as it interacted with Caspase 3 with a binding energy of −7.2 kcal/mol. MD simulation studies also revealed that Sciadopitysin was stable inside the binding pocket of Caspase 3 by interacting with the amino acids in the catalytic site thereby activating the Caspase 3 levels. By all the above results, Shivagutika could be used as a potent anti-breast cancer agent (specifically DCM extract of Shivagutika) which could decrease the cases of breast cancer in future.
Sub-micron microparticles with tunable fluorescence emission obtained via co-self-assembly of amidoximed polymeric ligands and lanthanide ions
Authors: Qiu Huandi , Ran Qimeng , Bai Yun , He Wei , Zheng Li , Pan Cong , Jia Kun , Hu Yiguo 
Published: May 2023

Lanthanide coordinating polymeric microparticles have witnessed increasing research interests during the past decades due to their versatile morphology and tunable fluorescent properties. Herein, we have synthesized an amidoximed block copolymer containing aromatic backbone and pendent amidoxime as well as carboxyl groups, which has been employed as the ligand to sensitize the intrinsic fluorescence emission of lanthanide ions of Tb3+ and Eu3+. Furthermore, the lanthanide coordinating polymeric microparticles showing tunable green and red emission fluorescence have been prepared via the emulsion confinement co-self-assembly of amidoximed polymeric ligands with Tb3+ and Eu3+. It is found that both the fluorescence emission and sizes of obtained fluorescent microparticles can be easily modulated in a wide range by tuning concentration of polymers and lanthanide ions, as well as emulsion evaporation temperature. Thanks to their tunable sizes (250–900 nm), fluorescence emission as well as presence of surface active functional groups, the present fluorescent microparticles would find potential applications in in-vitro detection, optical encoding and devices.
Amphotericin B colloidal dispersion: an effective drug for the treatment of mucormycosis in China
Authors: Liu Juntao , Ma Xiaoxu 
Published: May 2023

ObjectiveMucormycosis has emerged as an increasingly important cause of morbidity and mortality in immunocompromised patients, but the effective drugs for the treatment are limited. Hence, the study aimed to summarize the characteristics of mucormycosis in patients with hematological malignancies, and investigate the efficacy and safety of Amphotericin B Colloidal Dispersion (ABCD) in treating mucormycosis.MethodsIn this study, patients with mucormycosis complicated by hematological malignancies who received ABCD at the First Affiliated Hospital of Zhengzhou University from April 2021 to May 2022 were retrospectively enrolled. The clinical data of the enrolled patients were collected, and then, the drug response at 2 weeks, 4 weeks, and the end of treatment; the survival rate at 4, 8, and 12 weeks; and the laboratory-related indicators and adverse events (AEs) associated with ABCD were evaluated.ResultsIn total, 9 patients with mucormycosis complicated by hematological malignancies were enrolled. The main symptoms were fever, cough, and chest pain. In addition, reversed halo signs (RHS) were found on chest CTs. The responses to ABCD at 2 weeks, 4 weeks, and the end of treatment were 100% (9/9), 77.8% (7/9), and 77.8% (7/9), respectively. The survival rates of the patients at 4, 8, and 12 weeks were 77.8% (7/9), 66.7% (6/9), and 66.7% (6/9), respectively. Among laboratory-related indicators, white blood cell (WBC) counts were significantly increased from baseline after 1 and 2 weeks of ABCD treatment (P&lt;0.05), whereas neutrophil counts were only increased significantly from baseline at 2 weeks post-treatment (P&lt;0.05). The most common AEs were infusion-related AEs manifesting as fever, chills, and pruritus. Moreover, none of the patients suffered from renal injury once again.ConclusionABCD is a promising treatment strategy for patients with mucormycosis complicated by hematologic malignancies, showing remarkable efficacy and safety.
Circulating free T3 associates longitudinally with cardio-metabolic risk factors in euthyroid children with higher TSH
Authors: Carreras-Badosa Gemma , Puerto-Carranza Elsa , Mas-Parés Berta , Gómez-Vilarrubla Ariadna , Cebrià-Fondevila Helena , Díaz-Roldán Ferran , Riera-Pérez Elena , de Zegher Francis , Ibañez Lourdes , Bassols Judit , López-Bermejo Abel 
Published: May 2023

IntroductionThyroid hormones play major roles in the regulation of body composition and metabolism, and therefore, the relationship between thyroid hormones and cardio-metabolic risk has been extensively studied in adults. In this study, we aimed to test whether free triiodothyronine (fT3) associates longitudinally with cardio-metabolic risk factors in euthyroid children.MethodsA prospective study cohort of 599 apparently healthy school-age children were assessed at baseline (mean age 8.1 ± 2.1 years), of whom 270 children were also assessed at follow-up (4 years later). Circulating thyroid-stimulating hormone (TSH), free thyroxine (fT4), and fT3 were measured, and cardio-metabolic risk was assessed by means of body mass index (BMI), waist circumference, visceral fat (by ultrasound), blood pressure, circulating lipids, and homeostasis model assessment of insulin resistance (HOMA-IR) index, both at baseline and at follow-up.ResultsAll studied children had normal thyroid function tests. Independent associations between baseline fT3 and both baseline and follow-up BMI, systolic blood pressure, mean arterial blood pressure, triglycerides, and HOMA-IR were found using multivariate regression analysis (adjusting for sex and baseline age and BMI). Analyses of effect sizes showed that for each 1 unit-increase in baseline fT3 (pg/ml), follow-up BMI–standard deviation score (SDS) increased by 0.31 units (z-score) and systolic blood pressure by 6.6 units (mmHg). The observed longitudinal associations were more robust in children belonging to the upper TSH tertile who showed higher TSH levels and were characterized by weighing more and having the highest fT3 levels. In these children, for each 1 unit-increase in baseline fT3 (pg/ml), follow-up BMI-SDS increased by 0.67 units (z-score) and systolic blood pressure by 10.2 units (mmHg).ConclusionsCirculating fT3 associates longitudinally with cardio-metabolic risk factors in euthyroid children with higher TSH. The observed associations of thyroid hormones in these children could conceivably respond to a homeostatic attempt to reduce their cardio-metabolic risk.
A case-control study about markers of stress in normal-/overweight women with polycystic ovary syndrome and in controls
Authors: Marschalek Marie-Louise , Marculescu Rodrig , Schneeberger Christian , Marschalek Julian , Dewailly Didier , Ott Johannes 
Published: May 2023

BackgroundPolycystic ovary syndrome (PCOS) is linked to an elevated risk of psychological disorders, decreased quality of life and emotional distress. Serum cortisol as a potential stress marker has been found to be increased in women with PCOS. The aim of this study was to evaluate both saliva stress markers and subjective psychological distress in women with PCOS.MethodsIn a prospective case-control study, 31 PCOS women and 31 healthy controls were included. Salivary cortisol, and metanephrines were collected in the morning and in the evening. Emotional distress and quality of life were assessed by means of the Perceived Stress Scale (PSS-10) and the Short Form-36 (SF-36). Multivariable generalized linear models were applied to test the influence of various parameters on numerical outcome parameters.ResultsAfter correction for age and body mass index (BMI), there were no statistically significant differences of salivary biomarkers between PCOS women and healthy controls (p&gt;0.05). PCOS patients revealed significantly higher increased PSS total scores and lower quality of life in all SF-36 modules apart from pain (p&lt; 0.05). The PSS total score was positively correlated to prolactin in PCOS women (r= 0.450; p= 0.011). In overweight/obese PCOS patients, a higher BMI, a higher Ferriman Gallwey score and higher age significantly predicted the PSS total score (p&lt; 0.05).ConclusionStress measured by salivary biomarkers did not differ between PCOS women and healthy controls, whereas stress scores evaluated by questionnaires were significantly greater in women with PCOS. A higher BMI, hirsutism and a higher age seem to be the main modulators of subjective stress in PCOS. Prolactin might serve as a biomarker for chronic stress in PCOS women.
Caribbean scleractinian corals exhibit highly variable tolerances to acute hypoxia
Authors: Pontes Emma , Langdon Chris , Al-Horani Fuad A. 
Published: May 2023

IntroductionClimate change, and the increase in sea surface temperature, is exacerbating ocean deoxygenation because of the inherent property of seawater to sequester less dissolved gas, such as oxygen, at warmer temperatures. While most coral reef studies focus on the effects of thermal stress and ocean acidification, few studies acknowledge the threat of hypoxia. Hypoxia is traditionally defined as 6.3 kPa (2 mg L-1 O2), however, a universal hypoxia threshold is not useful given the vast range of responses among marine organisms. The range of metabolic responses and tolerances to hypoxia are unknown for Caribbean coral species and their algal symbionts.ObjectiveHere, we quantified the spectrum of acute hypoxia tolerances and the range of metabolic responses of six ecologically and structurally important Caribbean coral species (Acropora cervicornis, Siderastrea radians, Siderastrea siderea, Porites astreoides, Porites porites, and Orbicella faveolata) and their algal symbionts (Symbiodinium, Breviolum, and Durusdinium spp.).MethodsA total of 24 coral fragments (4 individuals per species) were exposed to 10 distinct oxygen concentrations ranging from normoxia (20.38 kPa) to severe hypoxia (3.3 kPa). We used intermittent flow respirometry to measure coral host respiration in the dark and algal symbiont photosynthesis in the light at each oxygen level. We determined a line of best fit for the metabolic rate vs. PO2 data and calculated the critical oxygen partial pressure (PO2 crit), a method that has not been tested on symbiotic species.ResultsCoral species and their algal symbionts measured here displayed a wide range of hypoxia tolerances. For the coral hosts, PO2 crit values differed roughly two-fold ranging from 5.74 kPa to 16.93 kPa, and for the algal symbionts, PO2 crit values differed roughly three-fold ranging from 3.9 kPa to 11.3 kPa.DiscussionThese results should be regarded as a first step to characterizing the metabolic response and acute tolerance of multiple coral hosts and algal symbionts to a wide range of oxygen concentrations. Given that some PO2 crit values were above the generally accepted hypoxia threshold, these results have implications for the community composition of reefs under a rapidly changing climate and can guide purposeful reef restoration.
Targeted transcriptomic analysis of pancreatic adenocarcinoma in EUS-FNA samples by NanoString technology
Authors: Pedrosa L. , Araujo I. K. , Cuatrecasas M. , Soy G. , López S. , Maurel J. , Sánchez-Montes C. , Montironi C. , Saurí T. , Sendino O. , Pérez F. M. , Ausania F. , Fernández-Esparrach G. , Espósito F. M. , Vaquero E. C. , Ginès A. 
Published: May 2023

Background: Integration of transcriptomic testing into EUS-FNA samples is a growing need for precision oncology in pancreatic ductal adenocarcinoma (PDAC). The NanoString platform is suitable for transcriptome profiling in low yield RNA samples.Methods: Inclusion of patients that underwent EUS-FNA cytological diagnosis of pancreatic ductal adenocarcinoma using 19G and/or 22G needles and subsequent surgical resection. Formalin-fixed, paraffin-embedded (FFPE) cytological and surgical samples underwent RNA extraction and transcriptomic analysis using a custom 52-gene NanoString panel of stromal PDAC features. Cell type abundance was quantified in FFPE specimens and correlated.Results: 18 PDAC patients were included. Mean EUS-FNA passes was 2 + 0.7. All FFPE passed the RNA quality control for genomic analysis. Hierarchical clustering on the global gene expression data showed that genes were differentially expressed between EUS and surgical samples. A more enriched cancer-associated fibroblasts and epithelial-mesenchymal transition transcriptomic profile was observed across surgical specimens whereas immunological biomarkers were more represented in EUS-FNA samples. Cytological examination confirmed a scanty representation of CAF and more immunological cell abundance in cytological samples in comparison to surgical specimens.Conclusion: Targeted transcriptomic NanoString profiling of PDAC samples obtained by EUS-FNA is a feasible approach for pre-surgical molecular analysis although stromal CAF/EMT mRNA biomarkers are underrepresented.
Spatial-temporal data-augmentation-based functional brain network analysis for brain disorders identification
Authors: Liu Qinghua , Zhang Yangyang , Guo Lingyun , Wang ZhengXia 
Published: May 2023

IntroductionDue to the lack of devices and the difficulty of gathering patients, the small sample size is one of the most challenging problems in functional brain network (FBN) analysis. Previous studies have attempted to solve this problem of sample limitation through data augmentation methods, such as sample transformation and noise addition. However, these methods ignore the unique spatial-temporal information of functional magnetic resonance imaging (fMRI) data, which is essential for FBN analysis.MethodsTo address this issue, we propose a spatial-temporal data-augmentation-based classification (STDAC) scheme that can fuse the spatial-temporal information, increase the samples, while improving the classification performance. Firstly, we propose a spatial augmentation module utilizing the spatial prior knowledge, which was ignored by previous augmentation methods. Secondly, we design a temporal augmentation module by random discontinuous sampling period, which can generate more samples than former approaches. Finally, a tensor fusion method is used to combine the features from the above two modules, which can make efficient use of spatial-temporal information of fMRI simultaneously. Besides, we apply our scheme to different types of classifiers to verify the generalization performance. To evaluate the effectiveness of our proposed scheme, we conduct extensive experiments on the Alzheimer's Disease Neuroimaging Initiative (ADNI) dataset and REST-meta-MDD Project (MDD) dataset.ResultsExperimental results show that the proposed scheme achieves superior classification accuracy (ADNI: 82.942%, MDD: 63.406%) and feature interpretation on the benchmark datasets.DiscussionThe proposed STDAC scheme, utilizing both spatial and temporal information, can generate more diverse samples than former augmentation methods for brain disorder classification and analysis.
Alzheimer’s disease pathogenetic progression is associated with changes in regulated retained introns and editing of circular RNAs
Authors: Arizaca Maquera Karol Andrea , Welden Justin Ralph , Margvelani Giorgi , Miranda Sardón Sandra C. , Hart Samantha , Robil Noémie , Hernandez Alvaro Gonzalo , de la Grange Pierre , Nelson Peter T. , Stamm Stefan 
Published: May 2023

IntroductionThe molecular changes leading to Alzheimer’s disease (AD) progression are poorly understood. A decisive factor in the disease occurs when neurofibrillary tangles (NFT) composed of microtubule associated protein tau (MAPT) form in the entorhinal cortex and then spread throughout the brain.MethodsWe therefore determined mRNA and circular RNA changes during AD progression, comparing Braak NFT stages I-VI. Total RNA was isolated from human brain (entorhinal and frontotemporal cortex). Poly(A)+ RNA was subjected to Nanopore sequencing, and total RNA was analyzed by standard Illumina sequencing. Circular RNAs were sequenced from RNase R treated and rRNA depleted total RNA. The sequences were analyzed using different bioinformatic tools, and expression constructs for circRNAs were analyzed in transfection experiments.ResultsWe detected 11,873 circRNAs of which 276 correlated with Braak NFT stages. Adenosine to inosine RNA editing increased about threefold in circRNAs during AD progression. Importantly, this correlation cannot be detected with mRNAs. CircMAN2A1 expression correlated with AD progression and transfection experiments indicated that RNA editing promoted its translation using start codons out of frame with linear mRNAs, which generates novel proteins.DiscussionThus, we identified novel regulated retained introns that correlate with NFT Braak stages and provide evidence for a role of translated circRNAs in AD development.