bluuebunny/crossref_metadata_2025_split huggingface dataset:
Peak Water Levels Rise Less Than Mean Sea Level in Tidal Channels Subject to Depth Convergence by Deepening
Authors: Leuven Jasper R. F. W. , Niesten Iris , Huismans Ymkje , Cox Jana R. , Hulsen Lamber , van der Kaaij Theo , Hoitink A. J. F. (Ton) 
Published: March 2023

AbstractEffects of sea‐level rise (SLR) on future peak water levels in tidal deltas and estuaries are largely unknown, despite these areas being densely populated and at high risk of flooding. While the rates of SLR accelerate, many channels simultaneously experience channel deepening for navigation. With globally decreasing sediment supplies, most channels are at risk of becoming deeper when the rate of SLR accelerates and sedimentation cannot keep pace with SLR. These factors potentially favor amplification of the tides and thereby increase flood risk, but the extent to which they will do so is unknown. Here, we introduce and use a validated model for an artificially deepened multi‐branch delta to get a mechanistic understanding of non‐linear SLR‐effects on peak water levels. Results show that, when the current deepened bed level will be maintained, peak water levels do not rise on par with mean sea‐level. Thus flood risk increases less than what can be expected from the predictions of the mean sea‐level increase. The reason is that SLR causes a proportional reduction in convergence of channel area. This mechanism reduces tidal amplification. Nevertheless, SLR effects extend far beyond the range of present‐day seasonal variations, with future low water levels being equal to present‐day high water levels, while the tidal range slightly reduces. This will have consequences not only for flood risk, but also for freshwater availability, navigation and ecology.
A Study of Simultaneous Assimilation of Coastal Ground‐Based and Airborne Radar Observations on the Prediction of Harvey (2017) With the Hourly 3DEnVar System for HWRF
Authors: Lu Xu , Wang Xuguang 
Published: March 2023

AbstractThis study investigates the relative impact of assimilating the ground‐based WSR‐88D radar (GBR) and the airborne Tail Doppler Radar (TDR) observations on the analysis and prediction of Hurricane Harvey (2017) during its landfalling stage. Results show that the assimilation of GBR (experiment “DAG”) outperforms the assimilation of TDR (experiment “DAT”) in multiple aspects. For example, “DAG” produces better predictions in Vmax, radial velocity, brightness temperature, and precipitation than “DAT.” The advantages of “DAG” over “DAT” are likely from the better analyzed thermodynamical structures in addition to the better data availability. The average Minimum Sea Level Pressure (MSLP) prediction error is the only aspect of “DAG” that is inferior to “DAT.” Diagnostics show that such an inferior performance of MSLP for “DAG” is associated with the systematic bias from the Hurricane Weather Research and Forecasting model. The combined assimilation of both observations (experiment “DAB”) shows complementary effects and performs the best overall among all experiments.
MAVEN Accelerometer Observations of Thermospheric Densities During Aerobraking and Deep Dip 2: Wave Features and Connections to Upward Propagating Thermal Tides
Authors: Jenkins G. , Bougher S. W. , Lugo R. , Tolson R. H. , Zurek R. W. , Baird D. , Steele L. , Kass D. , Withers P. 
Published: March 2023

AbstractIn early 2019, the Mars Atmosphere and Volatile Evolution (MAVEN) mission underwent an ∼2‐month aerobraking campaign during which time the spacecraft periapsis altitude was lowered from its nominal altitude range of 140–160 km to as low as ∼123 km. Excluding spacecraft walk‐in/out maneuvers, accelerometer measurements were made along 272 orbits with coverage spanning Ls 340–3°, latitudes ∼5°–54°S, longitudes 0–360°, and local solar time ∼22–17 hr. In this study, we perform a diagnostic analysis of the full aerobraking data set by fitting 4‐harmonic waves to mass densities. We then study the variations of these waves as a function of latitude with an emphasis on those observed previously in Mars' thermosphere by MAVEN and other missions. Additionally, we utilize data collected during the same time period from the Mars Reconnaissance Orbiter's Mars Climate Sounder to study the vertical propagation of waves originating from the middle atmosphere. Key results indicate that normalized wave amplitudes decrease with latitude, and this is consistent with the latitudinal structure of a diurnal Kelvin mode. We also observe that waves imprinted from the middle atmosphere show normalized amplitude growth with increasing altitude. A complete summary of data sets, analysis methodology, and scientific results is given. The purpose of this study is to add to the body of knowledge surrounding Martian atmospheric wave features and to provide further constraints for future numerical modeling and subsequent tidal mode identification.
Retrieving Accurate Precipitable Water Vapor Based on GNSS Multi‐Antenna PPP With an Ocean‐Based Dynamic Experiment
Authors: Wei Juan , Shu Yuanming , Liu Yang , Fang Rongxin , Qiao Lulu , Ding Dong , Li Guangxue , Liu Jingbin 
Published: March 2023

AbstractAs an attractive technique for measuring water vapor, the Global Navigation Satellite System (GNSS) faces additional challenges in dynamic applications such as in the open sea. We present a new method of retrieving precipitable water vapor (PWV) based on GNSS multi‐antenna precise point positioning (PPP), which uses GNSS data from multiple antennas and incorporates the constraints of known baseline vector and common tropospheric delay. The 4‐day shipborne dynamic experiment along the China coast demonstrates that the baseline vector constraint shortens the convergence time of positioning and atmospheric parameters, and also slightly improves their accuracies. The common tropospheric delay constraint helps to provide compromised, more robust, and sometimes more accurate PWV estimates. An evaluation with radiosonde‐derived PWVs shows that the combination of the two constraints achieves the best accuracy reaching 4.2 mm. This method helps to expand GNSS meteorology to the vast ocean and benefits satellite altimetry and weather forecasting.
From the teapot effect to tap-triggered self-wetting: a 3D self-driving sieve for whole blood filtration
Authors: Li Yuang , Li Xue , Zhang Lina , Luan Xiaofeng , Jiang Jiahong , Zhang Lingqian , Li Mingxiao , Wang Jinghui , Duan Jiangang , Zhao Haiping , Zhao Yang , Huang Chengjun 
Published: March 2023

AbstractAchieving passive microparticle filtration with micropore membranes is challenging due to the capillary pinning effect of the membranes. Inspired by the teapot effect that occurs when liquid (tea) is poured from a teapot spout, we proposed a tap-triggered self-wetting strategy and utilized the method with a 3D sieve to filter rare cells. First, a 3D-printed polymer tap-trigger microstructure was implemented. As a result, the 3 µm micropore membrane gating threshold (the pressure needed to open the micropores) was lowered from above 3000 to 80 Pa by the tap-trigger microstructure that facilated the liquid leakage and spreading to self-wet more membrane area in a positive feedback loop. Then, we implemented a 3D cone-shaped cell sieve with tap-trigger microstructures. Driven by gravity, the sieve performed at a high throughput above 20 mL/min (DPBS), while the micropore size and porosity were 3 µm and 14.1%, respectively. We further filtered leukocytes from whole blood samples with the proposed new 3D sieve, and the method was compared with the traditional method of leukocyte isolation by chemically removing red blood cells. The device exhibited comparable leukocyte purity but a higher platelet removal rate and lower leukocyte simulation level, facilitating downstream single-cell analysis. The key results indicated that the tap-triggered self-wetting strategy could significantly improve the performance of passive microparticle filtration.
Lysosomal Ca2+ as a mediator of palmitate-induced lipotoxicity
Authors: Oh Soo-Jin , Hwang Yeseong , Hur Kyu Yeon , Lee Myung-Shik 
Published: March 2023

AbstractWhile the mechanism of lipotoxicity by palmitic acid (PA), an effector of metabolic stress in vitro and in vivo, has been extensively investigated, molecular details of lipotoxicity are still not fully characterized. Since recent studies reported that PA can exert lysosomal stress in addition to well-known ER and mitochondrial stress, we studied the role of lysosomal events in lipotoxicity by PA, focusing on lysosomal Ca2+. We found that PA induced accumulation of mitochondrial ROS and that mitochondrial ROS induced release of lysosomal Ca2+ due to lysosomal Ca2+ exit channel activation. Lysosomal Ca2+ release led to increased cytosolic Ca2+ which induced mitochondrial permeability transition (mPT). Chelation of cytoplasmic Ca2+ or blockade of mPT with olesoxime or decylubiquinone (DUB) suppressed lipotoxicity. Lysosomal Ca2+ release led to reduced lysosomal Ca2+ content which was replenished by ER Ca2+, the largest intracellular Ca2+ reservoir (ER → lysosome Ca2+ refilling), which in turn activated store-operated Ca2+ entry (SOCE). Inhibition of ER → lysosome Ca2+ refilling by blockade of ER Ca2+ exit channel using dantrolene or inhibition of SOCE using BTP2 inhibited lipotoxicity in vitro. Dantrolene or DUB also inhibited lipotoxic death of hepatocytes in vivo induced by administration of ethyl palmitate together with LPS. These results suggest a novel pathway of lipotoxicity characterized by mPT due to lysosomal Ca2+ release which was supplemented by ER → lysosome Ca2+ refilling and subsequent SOCE, and also suggest the potential role of modulation of ER → lysosome Ca2+ refilling by dantrolene or other blockers of ER Ca2+ exit channels in disease conditions characterized by lipotoxicity such as metabolic syndrome, diabetes, cardiomyopathy or nonalcoholic steatohepatitis.
Supply chains create global benefits from improved vaccine accessibility
Authors: Wang Daoping , Bjørnstad Ottar N. , Lei Tianyang , Sun Yida , Huo Jingwen , Hao Qi , Zeng Zhao , Zhu Shupeng , Hallegatte Stéphane , Li Ruiyun , Guan Dabo , Stenseth Nils C. 
Published: March 2023

AbstractEnsuring a more equitable distribution of vaccines worldwide is an effective strategy to control global pandemics and support economic recovery. We analyze the socioeconomic effects - defined as health gains, lockdown-easing effect, and supply-chain rebuilding benefit - of a set of idealized COVID-19 vaccine distribution scenarios. We find that an equitable vaccine distribution across the world would increase global economic benefits by 11.7% ($950 billion per year), compared to a scenario focusing on vaccinating the entire population within vaccine-producing countries first and then distributing vaccines to non-vaccine-producing countries. With limited doses among low-income countries, prioritizing the elderly who are at high risk of dying, together with the key front-line workforce who are at high risk of exposure is projected to be economically beneficial (e.g., 0.9%~3.4% annual GDP in India). Our results reveal how equitable distributions would cascade more protection of vaccines to people and ways to improve vaccine equity and accessibility globally through international collaboration.
Cellular mechanisms of heterogeneity in NF2-mutant schwannoma
Authors: Chiasson-MacKenzie Christine , Vitte Jeremie , Liu Ching-Hui , Wright Emily A. , Flynn Elizabeth A. , Stott Shannon L. , Giovannini Marco , McClatchey Andrea I. 
Published: March 2023

AbstractSchwannomas are common sporadic tumors and hallmarks of familial neurofibromatosis type 2 (NF2) that develop predominantly on cranial and spinal nerves. Virtually all schwannomas result from inactivation of theNF2tumor suppressor gene with few, if any, cooperating mutations. Despite their genetic uniformity schwannomas exhibit remarkable clinical and therapeutic heterogeneity, which has impeded successful treatment. How heterogeneity develops inNF2-mutant schwannomas is unknown. We have found that loss of the membrane:cytoskeleton-associated NF2 tumor suppressor, merlin, yields unstable intrinsic polarity and enablesNf2−/−Schwann cells to adopt distinct programs of ErbB ligand production and polarized signaling, suggesting a self-generated model of schwannoma heterogeneity. We validated the heterogeneous distribution of biomarkers of these programs in human schwannoma and exploited the synchronous development of lesions in a mouse model to establish a quantitative pipeline for studying how schwannoma heterogeneity evolves. Our studies highlight the importance of intrinsic mechanisms of heterogeneity across human cancers.
Meta-analysis of the space flight and microgravity response of the Arabidopsis plant transcriptome
Authors: Barker Richard , Kruse Colin P. S. , Johnson Christina , Saravia-Butler Amanda , Fogle Homer , Chang Hyun-Seok , Trane Ralph Møller , Kinscherf Noah , Villacampa Alicia , Manzano Aránzazu , Herranz Raúl , Davin Laurence B. , Lewis Norman G. , Perera Imara , Wolverton Chris , Gupta Parul , Jaiswal Pankaj , Reinsch Sigrid S. , Wyatt Sarah , Gilroy Simon 
Published: March 2023

AbstractSpaceflight presents a multifaceted environment for plants, combining the effects on growth of many stressors and factors including altered gravity, the influence of experiment hardware, and increased radiation exposure. To help understand the plant response to this complex suite of factors this study compared transcriptomic analysis of 15Arabidopsis thalianaspaceflight experiments deposited in the National Aeronautics and Space Administration’s GeneLab data repository. These data were reanalyzed for genes showing significant differential expression in spaceflight versus ground controls using a single common computational pipeline for either the microarray or the RNA-seq datasets. Such a standardized approach to analysis should greatly increase the robustness of comparisons made between datasets. This analysis was coupled with extensive cross-referencing to a curated matrix of metadata associated with these experiments. Our study reveals that factors such as analysis type (i.e., microarray versus RNA-seq) or environmental and hardware conditions have important confounding effects on comparisons seeking to define plant reactions to spaceflight. The metadata matrix allows selection of studies with high similarity scores, i.e., that share multiple elements of experimental design, such as plant age or flight hardware. Comparisons between these studies then helps reduce the complexity in drawing conclusions arising from comparisons made between experiments with very different designs.
Does anxiety explain why math-anxious people underperform in math?
Authors: Daker Richard J. , Gattas Sylvia U. , Necka Elizabeth A. , Green Adam E. , Lyons Ian M. 
Published: March 2023

AbstractMath-anxious people consistently underperform in math. The most widely accepted explanation forwhythis underperformance occurs is that math-anxious people experience heightened anxiety when faced with math, and this in-the-moment anxiety interferes with performance. Surprisingly, this explanation has not been tested directly. Here, using both self-report and physiological indices of anxiety, we directly test how much in-the-moment anxiety explains math-anxious underperformance. Results indicate that in-the-moment anxiety indeed explains why math-anxious people underperform—but only partially, suggesting a need to seriously consider alternative mechanisms. Results also showed that while some highly math-anxious individuals—those with high levels of heart rate variability—experienced less in-the-moment anxiety, they nevertheless performed no better at math. For these individuals, math-anxious underperformance must occur for reasons unrelated to in-the-moment anxiety. More broadly, our findings point to substantial individual heterogeneity in the mechanisms underlying math-anxious underperformance. Accounting for this mechanistic heterogeneity may prove vital for optimally boosting math performance in math-anxious individuals.
A Timm-Nissl multiplane microscopic atlas of rat brain zincergic terminal fields and metal-containing glia
Authors: Blixhavn Camilla H. , Haug Finn-Mogens Š. , Kleven Heidi , Puchades Maja A. , Bjaalie Jan G. , Leergaard Trygve B. 
Published: March 2023

AbstractThe ability of Timm’s sulphide silver method to stain zincergic terminal fields has made it a useful neuromorphological marker. Beyond its roles in zinc-signalling and neuromodulation, zinc is involved in the pathophysiology of ischemic stroke, epilepsy, degenerative diseases and neuropsychiatric conditions. In addition to visualising zincergic terminal fields, the method also labels transition metals in neuronal perikarya and glial cells. To provide a benchmark reference for planning and interpretation of experimental investigations of zinc-related phenomena in rat brains, we have established a comprehensive repository of serial microscopic images from a historical collection of coronally, horizontally and sagittally oriented rat brain sections stained with Timm’s method. Adjacent Nissl-stained sections showing cytoarchitecture, and customised atlas overlays from a three-dimensional rat brain reference atlas registered to each section image are included for spatial reference and guiding identification of anatomical boundaries. The Timm-Nissl atlas, available from EBRAINS, enables experimental researchers to navigate normal rat brain material in three planes and investigate the spatial distribution and density of zincergic terminal fields across the entire brain.
A benchmark for machine-learning based non-invasive blood pressure estimation using photoplethysmogram
Authors: González Sergio , Hsieh Wan-Ting , Chen Trista Pei-Chun 
Published: March 2023

AbstractBlood Pressure (BP) is an important cardiovascular health indicator. BP is usually monitored non-invasively with a cuff-based device, which can be bulky and inconvenient. Thus, continuous and portable BP monitoring devices, such as those based on a photoplethysmography (PPG) waveform, are desirable. In particular, Machine Learning (ML) based BP estimation approaches have gained considerable attention as they have the potential to estimate intermittent or continuous BP with only a single PPG measurement. Over the last few years, many ML-based BP estimation approaches have been proposed with no agreement on their modeling methodology. To ease the model comparison, we designed a benchmark with four open datasets with shared preprocessing, the right validation strategy avoiding information shift and leak, and standard evaluation metrics. We also adapted Mean Absolute Scaled Error (MASE) to improve the interpretability of model evaluation, especially across different BP datasets. The proposed benchmark comes with open datasets and codes. We showcase its effectiveness by comparing 11 ML-based approaches of three different categories.
Effects of routine procedures on salivary cortisol in mechanically ventilated neonates
Authors: Li Wanting , Zhang Huiyue , Huang Xianghui , Ye Ruming , Lin Ying 
Published: March 2023

AbstractEven though the stress secondary to invasive procedures has been investigated, less attention has been paid to the stimulation or pain caused by routine procedures on neonates. The changes in salivary cortisol concentration in mechanically ventilated NICU patients during routine procedures were monitored to provide reference and guidance for pain management. 80 mechanically ventilated neonates in the hospital from Sep 2021 to Mar 2022 were selected. The salivary cortisol levels of the neonates were monitored during nursing procedures and were categorized by their risk levels to the following groups: high-risk (endotracheal suctioning and arterial blood sampling), moderate-risk (gastric tube insertion, venipuncture), and low-risk (bedside bathing and diaper changes). The changes in heart rate were also recorded and compared. The concentration of cortisol in the saliva of the neonates was 1.5 ± 0.8 nmol/L during the sleeping state, 6.2 ± 1.3 nmol/L during endotracheal suctioning, 6.4 ± 1.4 nmol/L during arterial blood sampling, 6.1 ± 1.2 nmol/L during venipuncture, 4.4 ± 1.1 nmol/L during gastric tube insertion, 3.5 ± 0.8 nmol/L during bedside bathing, and 3.3 ± 0.9 nmol/L during a diaper change. The results revealed a statistically significant effect between routine procedures on salivary cortisol levels. Compared with the neonates in the control sleep state, there was a significant (P < 0.05) change in salivary cortisol concentration of infants undergoing high and moderate-risk nursing procedures. There was a small but significant (P < 0.05) change in salivary cortisol levels in infants who underwent low-risk procedures compared to infants in the control sleep state. Further, the fluctuation of salivary cortisol levels in routine procedures was more frequent compared with routine handling at night. The fluctuations of salivary cortisol concentration in high-risk procedures were larger than that of infants who underwent low-risk procedures, with the difference being statistically significant (P < 0.05). It was also determined that the top four influencing factors on the infants’ heart rate were arterial blood sampling venipuncture, intubation, endotracheal suctioning, and gastric tube insertion (P < 0.05). Monitoring the saliva cortisol concentration index and heart rates can reflect the impact of different routine procedures on newborns and can be used to manage neonatal pain in the future.
Intradermal needle-free injection prevents African Swine Fever transmission, while intramuscular needle injection does not
Authors: Salman Muhammad , Lin Hongyao , Suntisukwattana Roypim , Watcharavongtip Parin , Jermsutjarit Patumporn , Tantituvanont Angkana , Nilubol Dachrit 
Published: March 2023

AbstractShared needles are a possible iatrogenic and hematogenous inanimate vector of African Swine Fever virus (ASFV) in farm conditions. To evaluate that possible transmission, sixty, 4-week-old pigs were procured from an ASF free herd free. Upon arrival, pigs were randomly divided into two sets. Set 1 served as seeder pigs, and were randomly allocated to 4 groups. The other pigs were divided into 8 groups, and served as sentinels. Seeder pigs were oronasally challenged with ASFV at high (108 copy numbers/mL), moderate (106 copy numbers/mL) or low (101 copy numbers/mL) challenge titer, except a subgroup that remained unchallenged (negative control). At 7 days post challenge (peak viremia), all four seeder groups were intradermally and intramuscularly (IM) injected with a vaccine adjuvant (Diluvac Forte, MSD Animal Health, The Netherlands) using a needle-free device (IDAL 3G, MSD Animal Health, The Netherlands) and conventional needles, respectively. The same needle or needle-free device was then used to inject the same volume of adjuvant into set 2 (n = 48) pigs. All pigs were observed for clinical disease daily and assayed for the presence of ASFV DNA by quantitative PCR. All seeder groups developed viremia (except the control pigs). ASFV viremia was detected in all sentinel groups injected via the intramuscular route. Transmission rate from the IM route via conventional needles was positively correlated with virus titer in blood circulation of seeders. Sentinels intramuscularly exposed to needles from high titer challenged seeders displayed more severe and acute clinical disease compared to that of exposed to low titer challenged seeders. No viremia nor clinical signs were observed in the sentinel groups injected via the intradermal route. This study confirmed the hematogenous transmission of ASFV between pigs through needle-sharing.