bluuebunny/crossref_metadata_2025_split huggingface dataset:
Innovative biosynthesis, artificial intelligence-based optimization, and characterization of chitosan nanoparticles by Streptomyces microflavus and their inhibitory potential against Pectobacterium carotovorum
Authors: El-Naggar Noura El-Ahmady , Bashir Shimaa I. , Rabei Nashwa H. , Saber WesamEldin I. A. 
Published: December 2022

AbstractMicrobial-based strategy in nanotechnology offers economic, eco-friendly, and biosafety advantages over traditional chemical and physical protocols. The current study describes a novel biosynthesis protocol for chitosan nanoparticles (CNPs), employing a pioneer Streptomyces sp. strain NEAE-83, which exhibited a significant potential for CNPs biosynthesis. It was identified as Streptomyces microflavus strain NEAE-83 based on morphological, and physiological properties as well as the 16S rRNA sequence (GenBank accession number: MG384964). CNPs were characterized by SEM, TEM, EDXS, zeta potential, FTIR, XRD, TGA, and DSC. CNPs biosynthesis was maximized using a mathematical model, face-centered central composite design (CCFCD). The highest yield of CNPs (9.41 mg/mL) was obtained in run no. 27, using an initial pH of 5.5, 1% chitosan, 40 °C, and a 12 h incubation period. Innovatively, the artificial neural network (ANN), was used for validating and predicting CNPs biosynthesis based on the trials data of CCFCD. Despite the high precision degree of both models, ANN was supreme in the prediction of CNPs biosynthesis compared to CCFCD. ANN had a higher prediction efficacy and, lower error values (RMSE, MDA, and SSE). CNPs biosynthesized by Streptomyces microflavus strain NEAE-83 showed in-vitro antibacterial activity against Pectobacterium carotovorum, which causes the potato soft rot. These results suggested its potential application for controlling the destructive potato soft rot diseases. This is the first report on the biosynthesis of CNPs using a newly isolated; Streptomyces microflavus strain NEAE-83 as an eco-friendly approach and optimization of the biosynthesis process by artificial intelligence.
Machine learning approaches demonstrate that protein structures carry information about their genetic coding
Authors: Ackerman-Schraier Linor , Rosenberg Aviv A. , Marx Ailie , Bronstein Alex M. 
Published: December 2022

AbstractSynonymous codons translate into the same amino acid. Although the identity of synonymous codons is often considered inconsequential to the final protein structure, there is mounting evidence for an association between the two. Our study examined this association using regression and classification models, finding that codon sequences predict protein backbone dihedral angles with a lower error than amino acid sequences, and that models trained with true dihedral angles have better classification of synonymous codons given structural information than models trained with random dihedral angles. Using this classification approach, we investigated local codon–codon dependencies and tested whether synonymous codon identity can be predicted more accurately from codon context than amino acid context alone, and most specifically which codon context position carries the most predictive power.
Study on hydraulic spray atomizing system as a new resource-efficient dyeing-finishing method for wool fabric
Authors: Mulder Roos , Morshed Mohammad Neaz , Seipel Sina , Norén Ulrika , Niit Ellinor , Nierstrasz Vincent 
Published: December 2022

AbstractThis study introduces hydraulic spray (HS) atomizing system as new resource-efficient continuous dyeing-finishing method for wool fabric. Here, wool fabric was dyed and finished by using commercial dyes and finishes through either one-step or two-steps HS method. Results obtained from color strength (K/S), color difference (ΔECMC) and color fastness analysis presented the apprehension of HS method in dyeing of wool fabric with different GSM and dyes. Finishing performance of wool fabric was measured through water contact angle analysis. Analysis shows that, the finishing performance of HS method were substantial to reach water contact angle as high as 145° while maintaining high fastness to wash and abrasion. Between one-step and two-steps HS method, one-step method showed better performance with high resource efficiency compared to two-steps method. Results from statistical analysis shows no statistical significance of fabric weight, type of dyes, and finishes to the performance of new HS method which is crucial for true-scale industrial implementation and scaling up of this process. The findings of this report are of great importance as it presents a greener alternative to the conventional resource-intensive dyeing-finishing methods of wool fabric.
Effects of coiling embolism on blood hemodynamic of the MCA aneurysm: a numerical study
Authors: Valipour Peiman 
Published: December 2022

AbstractOne of common endovascular technique for treatment of MCA aneurysm is using coiling gel for limiting of blood stream. In this work, computational fluid dynamic is used for the simulation of the blood hemodynamic inside MCA in existence of coiling gel. This work has tried to visualize the impacts of blood characteristics i.e. hematocrit as a protein related factor on efficiency of coiling fiber inside the aneurysm. Tufts of polyester fibers may be attached to the coil to support thrombosis and platelet aggregation. Blood rheology analysis is done by solving RANS equations and it is assumed that blood stream is non-Newtonian with fluid–solid interaction. OSI and WSS are compared on sac surface area for different stages of blood cycle. Achieved results confirm that the coiling gel substantially decreases the blood circulation inside the aneurysm sac. It is also found that the influence of blood hematocrit decreases when the MCA aneurysm is filled by the coiling gel.
Risk factors associated with Retinopathy of Prematurity development and progression
Authors: de las Rivas Ramírez Nieves , Luque Aranda Guillermo , Rius Díaz Francisca , Pérez Frías Francisco Javier , Sánchez Tamayo Tomás 
Published: December 2022

AbstractSeveral studies propose that Retinopathy of Prematurity (ROP) is a multifactorial disorder implicating many prenatal and postnatal factors. The objective of our study was to determine the incidence and the risk factors that influenced ROP development and progression. We retrospectively compiled data of preterms with birth weight (BW) ≤ 1.500 g and/or gestational age (GA) < 32 weeks, or BW between 1.501 and 2.000 g and/or GA ≥ 32 weeks with oxygen supply > 72 h or unstable clinical course screened for ROP in Regional University Hospital of Málaga from 2015 to 2018. 202 infants (44.7%) developed ROP and 66 exhibited progression (32.7% of ROP infants). In the univariate analysis, many risk factors were associated with ROP. In the subsequent multivariate analysis, GA, oxygen therapy and weight at 28 days of life, mechanical ventilation duration, non-invasive ventilation, surfactant administration and late-onset sepsis were independently associated with the development. However, oxygen therapy duration, late-onset sepsis and weight at 28 days were associated with the progression. The ROP development and progression risk factors were different. Our results are important to facilitate screening, early diagnosis and ROP treatment while reducing unneeded examinations.
Emotional eating and disordered eating behaviors in children and adolescents with type 1 diabetes
Authors: Ripoli Carlo , Ricciardi Maria Rossella , Zuncheddu Ester , Angelo Maria Rosaria , Pinna Anna Paola , Ripoli Daniela 
Published: December 2022

AbstractDisordered eating behaviors (DEB) are more common in adolescents with type 1 diabetes (T1D) than in peers without diabetes. Emotional eating is a risk factor for binge eating in children and adolescents in the general population and is associated with increased intake of high energy-dense foods rich in sugars and fats. The primary objective is to evaluate whether emotional eating is associated with the metabolic control (glycated hemoglobin, plasma lipids and uric acid) in children and adolescents with type 1 diabetes and whether subjects with DEB (DEPS-R ≥ 20) have higher emotional eating than those without DEB. The secondary objective is to evaluate whether emotional eating is associated with the different symptoms of DEB. Emotional eating is positively correlated with HbA1c, total and LDL cholesterol values in children and adolescents with T1D. Subjects with DEB have a higher emotional eating score than subjects without DEB. Disinhibition is the most common disordered eating behavior in children and adolescents with T1D and is associated with a higher emotional eating score. Early identification and treatment of emotional eating could be tools for preventing DEB in people with type 1 diabetes. A total of 212 adolescents with T1D completed two self-administered questionnaires: the Diabetes Eating Problem Survey-Revised (DEPS-R) and the Emotional Eating Scale for Children and Adolescents (EES-C). Demographic (age, sex, duration of the disease), anthropometric (weight, height, BMI, BMI-SDS), therapeutic (type of insulin therapy, daily insulin dose) and metabolic (HbA1c, total cholesterol, HDL, LDL, triglycerides, uric acid) data were taken from the patients' medical records. The presence of other autoimmune diseases was also recorded.
Acoustic speech features in social comparison: how stress impacts the way you sound
Authors: Kappen Mitchel , van der Donckt Jonas , Vanhollebeke Gert , Allaert Jens , Degraeve Vic , Madhu Nilesh , Van Hoecke Sofie , Vanderhasselt Marie-Anne 
Published: December 2022

AbstractThe use of speech as a digital biomarker to detect stress levels is increasingly gaining attention. Yet, heterogeneous effects of stress on specific acoustic speech features have been observed, possibly due to previous studies’ use of different stress labels/categories and the lack of solid stress induction paradigms or validation of experienced stress. Here, we deployed a controlled, within-subject psychosocial stress induction experiment in which participants received both neutral (control condition) and negative (negative condition) comparative feedback after solving a challenging cognitive task. This study is the first to use a (non-actor) within-participant design that verifies a successful stress induction using both self-report (i.e., decreased reported valence) and physiological measures (i.e., increased heart rate acceleration using event-related cardiac responses during feedback exposure). Analyses of acoustic speech features showed a significant increase in Fundamental Frequency (F0) and Harmonics-to-Noise Ratio (HNR), and a significant decrease in shimmer during the negative feedback condition. Our results using read-out-loud speech comply with earlier research, yet we are the first to validate these results in a well-controlled but ecologically-valid setting to guarantee the generalization of our findings to real-life settings. Further research should aim to replicate these results in a free speech setting to test the robustness of our findings for real-world settings and should include semantics to also take into account what you say and not only how you say it.
Antimicrobial and anti-biofilm activity of Polygonum chinense L.aqueous extract against Staphylococcus aureus
Authors: Zeng Jianye , Chen Dandan , Lv Chunli , Qin Kening , Zhou Qin , Pu Na , Song Shanshan , Wang Xiaomin 
Published: December 2022

AbstractPolygonum chinense Linn. (Polygonum chinense L.) is one of the main raw materials of Chinese patent medicines such as Guangdong herbal tea. The increasing antibiotic resistance of S. aureus and the biofilm poses a serious health threat to humans, and there is an urgent need to provide new antimicrobial agents. As a traditional Chinese medicine, the antibacterial effect of Polygonum chinense L. has been reported, but the antibacterial mechanism of Polygonum chinense L.aqueous extract and its effect on biofilm have not been studied in great detail, which hinders its application as an effective antibacterial agent. In this study, the mechanism of action of Polygonum chinense L.aqueous extract on Staphylococcus aureus (S. aureus) and its biofilm was mainly evaluated by morphological observation, flow cytometry and laser confocal experiments. Our findings demonstrate that Polygonum chinense L.aqueous extract has a significant bacteriostatic effect on S. aureus. The result of growth curve exhibits that Polygonum chinense L.aqueous extract presents a significant inhibitory effect against S. aureus. Transmission electron microscopy (TEM) and scanning electron microscopy (SEM) reveals that Polygonum chinense L.aqueous extract exerts a potent destruction of the cell wall of S. aureus and a significant inhibitory effect on the formation of S. aureus biofilm. In addition, flow cytometry showed the ability of Polygonum chinense L.aqueous extract to promote apoptosis by disrupting cell membranes of S. aureus. Notably, confocal laser scanning microscopy (CLSM) images illustrated the ability of Polygonum chinense L.aqueous to inhibit the formation of S. aureus biofilms in a dose-dependent manner. These results suggested that Polygonum chinense L.aqueous is a promising alternative antibacterial and anti-biofilm agent for combating infections caused by planktonic and biofilm cells of S. aureus.
Planetary transmission performance tests at very low temperatures
Authors: Sikorski Jakub , Pawlowski Witold 
Published: December 2022

AbstractThis article presents the results of a study on resistance to motion in a multi-stage planetary transmission, built with lightweight structural materials such as aluminum alloy 2017, with bearing nodes featuring steel ball bearings made from X65Cr14 alloy and lubricated with molybdenum disulfide powder. Details of the planetary gear construction were presented, followed by operational performance tests. During the performance tests, the temperature of the running transmission was gradually lowered with liquid nitrogen to as low as − 190 °C. The analysis covered, among others, the power consumption of the mechanism as a function of temperature. The results were compared with the parameters of the mechanisms already working in space. The measurements were carried out to confirm the applicability of the gearing in drive systems of manipulators intended to operate in open space or under extraterrestrial conditions such as on Mars.
Exploratory factor analysis determines latent factors in Guillain–Barré syndrome
Authors: Omura Seiichi , Shimizu Kazuaki , Kuwahara Motoi , Morikawa-Urase Miyuki , Kusunoki Susumu , Tsunoda Ikuo 
Published: December 2022

AbstractExploratory factor analysis (EFA) has been developed as a powerful statistical procedure in psychological research. EFA’s purpose is to identify the nature and number of latent constructs (= factors) underlying a set of observed variables. Since the research goal of EFA is to determine what causes the observed responses, EFA is ideal for hypothesis-based studies, such as identifying the number and nature of latent factors (e.g., cause, risk factors, etc.). However, the application of EFA in the biomedical field has been limited. Guillain–Barré syndrome (GBS) is peripheral neuropathy, in which the presence of antibodies to glycolipids has been associated with clinical signs. Although the precise mechanism for the generation of anti-glycolipid antibodies is unclear, we hypothesized that latent factors, such as distinct autoantigens and microbes, could induce different sets of anti-glycolipid antibodies in subsets of GBS patients. Using 55 glycolipid antibody titers from 100 GBS and 30 control sera obtained by glycoarray, we conducted EFA and extracted four factors related to neuroantigens and one potentially suppressive factor, each of which was composed of the distinct set of anti-glycolipid antibodies. The four groups of anti-glycolipid antibodies categorized by unsupervised EFA were consistent with experimental and clinical findings reported previously. Therefore, we proved that unsupervised EFA could be applied to biomedical data to extract latent factors. Applying EFA for other biomedical big data may elucidate latent factors of other diseases with unknown causes or suppressing/exacerbating factors, including COVID-19.
Current dependence of the negative magnetoresistance in superconducting NbN nanowires
Authors: Sofer Zoharchen , Shaulov Avner , Yeshurun Yosef 
Published: December 2022

AbstractMagnetoresistance measurements in amorphous NbN nanowires show that transport current affects their negative magnetoresistance (nMR) in a manner qualitatively similar to temperature. In particular, the current suppresses the nMR and, beyond a certain level it eliminates the effect altogether. As the temperature dependence of the nMR effect is more pronounced at low currents, similarly the current dependence of the effect is more pronounced at low temperatures. These results are discussed in terms of the phenomenological model which attributes the nMR to the interplay between the resistance originating from the rate of phase slips via the Josephson relation and the Ohmic contribution from quasiparticles charge imbalance that accompany fluctuations of the order parameter in the nanowire.
The effects of temperature stress and population origin on the thermal sensitivity of Lymantria dispar L. (Lepidoptera: Erebidae) larvae
Authors: Ilijin Larisa , Grčić Anja , Mrdaković Marija , Vlahović Milena , Todorović Dajana , Filipović Aleksandra , Matić Dragana , Perić Mataruga Vesna 
Published: December 2022

AbstractIncreased environmental temperature is one of the most frequent stresses effecting metabolic rate in herbivorous insect species. Our goal was to compare the influence of increased environmental temperature and induced thermotolerance on the activity of midgut phosphatases and brain tissue hsp70 concentration in 5th instar Lymantria dispar larvae originating from an unpolluted and polluted forest. Induced thermotolerance (larval pre-treatment at high, sub-lethal temperature) increases the species ability to overcome the negative effects of thermal stress, therefore we monitored the effect of this regime in larvae originating from both forests. Thermal regimes in this experiment predominantly influenced the alkaline phosphatases activity and it was affected by temperature, population origin, and their combined effect. Total acid phosphatases activity was changed only by the joint effect of temperature and population origin. Brain hsp70 concentration was under a significant individual and joint effect of temperature and population. In both populations, brain tissue hsp70 concentration and alkaline phosphatases activity should be taken under consideration as a battery with biomarker potential for thermal stress in L. dispar larvae as a bioindicator species.
Development and validation of a nomogram for predicting Mycoplasma pneumoniae pneumonia in adults
Authors: Ren Yuan , Wang Yan , Liang Ruifeng , Hao Binwei , Wang Hongxia , Yuan Jianwei , Wang Lin , Guo Zhizun , Zhang Jianwei 
Published: December 2022

AbstractThe study aimed to explore predictors of Mycoplasma pneumoniae pneumonia (MPP) in adults and develop a nomogram predictive model in order to identify high-risk patients early. We retrospectively analysed the clinical data of a total of 337 adult patients with community-acquired pneumonia (CAP) and divided them into MPP and non-MPP groups according to whether they were infected with MP. Univariate and multivariate logistic regression analyses were used to screen independent predictors of MPP in adults and to developed a nomogram model. Receiver operating characteristic (ROC) curve, calibration curve, concordance index (C-index), and decision curve analysis (DCA) were used for the validation of the evaluation model. Finally, the nomogram was further evaluated by internal verification. Age, body temperature, dry cough, dizziness, CRP and tree-in-bud sign were independent predictors of MPP in adults (P < 0.05). The nomogram showed high accuracy with C-index of 0.836 and well-fitted calibration curves in both the training and validation sets. The area under the receiver operating curve (AUROC) was 0.829 (95% CI 0.774–0.883) for the training set and 0.847 (95% CI 0.768–0.925) for the validation set. This nomogram prediction model can accurately predict the risk of MPP occurrence in adults, which helps clinicians identify high-risk patients at an early stage and make drug selection and clinical decisions.
Preventive residual insecticide applications successfully controlled Aedes aegypti in Yucatan, Mexico
Authors: Vazquez-Prokopec Gonzalo M. , Che-Mendoza Azael , Kirstein Oscar D. , Bibiano-Marin Wilberth , González-Olvera Gabriela , Medina-Barreiro Anuar , Gomez-Dantes Hector , Pavia-Ruz Norma , Manrique-Saide Pablo 
Published: December 2022

AbstractInsecticide-based approaches remain a key pillar for Aedes-borne virus (ABV, dengue, chikungunya, Zika) control, yet they are challenged by the limited effect of traditional outdoor insecticide campaigns responding to reported arboviral cases and by the emergence of insecticide resistance in mosquitoes. A three-arm Phase II unblinded entomological cluster randomized trial was conducted in Merida, Yucatan State, Mexico, to quantify the entomological impact of targeted indoor residual spraying (TIRS, application of residual insecticides in Ae. aegypti indoor resting sites) applied preventively 2 months before the beginning of the arbovirus transmission season. Trial arms involved the use of two insecticides with unrelated modes of action (Actellic 300CS, pirimiphos-methyl, and SumiShield 50WG, clothianidin) and a control arm where TIRS was not applied. Entomological impact was quantified by Prokopack adult collections performed indoors during 10 min per house. Regardless of the insecticide, conducting a preventive TIRS application led to significant reductions in indoor Ae. aegypti densities, which were maintained at the same levels as in the low arbovirus transmission period (Actellic 300CS reduced Ae. aegypti density up to 8 months, whereas SumiShield 50WG up to 6 months). The proportional reduction in Ae. aegypti abundance in treatment houses compared to control houses was 50–70% for Actellic 300CS and 43–63% for SumiShield 50WG. Total operational costs including insecticide ranged from US$4.2 to US$10.5 per house, depending on the insecticide cost. Conducting preventive residual insecticide applications can maintain Ae. aegypti densities at low levels year-round with important implications for preventing ABVs in the Americas and beyond.