bluuebunny/crossref_metadata_2025_split huggingface dataset:
Process Parameters Effect and Porosity Reduction on AlSi10Mg Parts Manufactured by Selective Laser Melting
Authors: Rios July Andrea Andrea Taborda , Robledo Patricia del Carmen Zambrano , Taborda Juan Daniel Trejos , Espinoza Johan Andres Gómez , Isaza Catalina Jaramillo , Hernández Arturo Juárez 
Published: May 2023

Abstract
A study was carried out about the processing parameters influence of an AlSi10Mg part manufactured by selective laser melting process in order to determine its optimal values and this way to improve the mechanical performance. For this purpose, a Design-of-Experiment was realized by the Taguchi method selecting a set of appro
priate processing parameters (9 combinations), resulting in 45 samples whose relative densities were measured by Archimedes’ principle. The results showed that a relative density of up to 99.65 % was achieved from raw data. While the statistical model by the Taguchi method indicated a comprehensive relationship between process parameters based on relative density, being laser power the major significance among the parameters. The Taguchi model suggested new values of process parameters, achieving AlSi10Mg parts with 99.98 % of relative density. To contrast the mechanical properties of both optimized and non-optimized parts, they were characterized by tension testing.
Identification of a brand intratumor microbiome signature for predicting prognosis of hepatocellular carcinoma
Authors: Song Yisu , Xiang Ze , Lu Zhengyang , Su Renyi , Shu Wenzhi , Sui Meihua , Wei Xuyong , Xu Xiao 
Published: May 2023

Abstract
Purpose Given that prognosis of hepatocellular carcinoma (HCC) differs dramatically, it is imperative to uncover effective and available prognostic biomarker(s). The intratumor microbiome plays a significant role in the response to tumor microenvironment, we aimed to identify an intratumor microbiome signature for predicting the prognosis of HCC patients accurately and investigate its possible mechanisms subsequently.Methods The TCGA HCC microbiome data (TCGA-LIHC-microbiome) was downloaded from cBioPortal. To create an intratumor microbiome related prognostic signature, univariate and multivariate Cox regression analyses were used to quantify the association of microbial abundance and patients’ overall survival (OS), as well as their diseases specific survival (DSS). The performance of the scoring model was evaluated by the area under the ROC curve (AUC). Based on the microbiome related signature, clinical factors, and multi-omics molecular subtypes on the basis of “icluster” algorithm, nomograms were established to predict OS and DSS. Patients were further clustered into three subtypes based on their microbiome related characteristics by consensus clustering. Moreover, deconvolution algorithm, weighted correlation network analysis (WGCNA) and gene set variation analysis (GSVA) were used to investigate the potential mechanisms.Results In TCGA LIHC microbiome data, the abundances of 166 genera among the total 1406 genera were considerably associated with HCC patients’ OS. From that filtered dataset we identified a 27-microbe prognostic signature and developed a microbiome related score (MRS) model. Compared with those in relatively low risk group, patients in higher risk group own a much worse OS(P < 0.0001). Besides, the time-dependent ROC curves with MRS showed excellent predictive efficacy both in OS and DSS. Moreover, MRS is an independent prognostic factor for OS and DSS over clinical factors and multi-omics based molecular subtypes. The integration of MRS into nomograms significantly improved the efficacy of prognosis prediction (1 year AUC:0.849, 3 year AUC: 0.825, 5-year AUC: 0.822). The analysis of microbiome-based subtypes on their immune characteristics and specific gene modules inferred that intratumor microbiome may affect the HCC patients’ prognosis via modulating the cancer stemness and immune response.Conclusion MRS, a 27 intratumor microbiome related prognostic model, was successfully established to predict HCC patients overall survive independently. And the possible underlying mechanisms were also investigated to provide a potential intervention strategy.
Waste Tyre Pieces as Photothermal Absorbers for Efficient Evaporation in Solar Desalination Systems
Authors: Suraparaju Subbarama Kousik , Natarajan Sendhil Kumar 
Published: May 2023

Abstract
Valarozition and reusing of waste materials before recycling would give sustainable outcomes and reduce the environmental effects. Among various waste materials, automobile tyres are one of the major contributors to environmental pollution while burning as they contain rubber. Hence, worn automobile tyres are procured and made into small pieces before burning to investigate the photothermal absorption in solar thermal applications. In the available solar thermal applications, solar still is the cheap and uncomplicated desalination approach that can be accessible by everyone. However, clean water production is very low in conventional solar still. Therefore, the key intention of this investigation is to improve pure drinking water generation by floating black tyre pieces in the absorber basin of solar still, which has better heat absorption characteristics. The outcomes reported that the clean water production in solar still with tyre pieces (SS-Tyre) is enhanced by 18% more than that of conventional solar still (CSS). The clean water production from SS-Tyre is about 2.7 l/sq.mt, Whereas the distillate output of CSS, is about 2.3 l/sq.mt on two consecutive testing days. In addition to productivity, the cost assessment reported that the cost per litre (CPL) and Payback Time (PT) of SS-Tyre are 19.2% and 20.1% lesser than CSS. Also, the environmental impact assessment of CSS and SS-Tyre reported that the SS-Tyre has 19% more net carbon credit than CSS.
Global screening and genetic engineering of Tistrella enable sustainable production of didemnin drugs
Authors: Tang Xiaoyu , Zhang Haili , Hui Zhen , Cai Mingwei , Zou Xiaolin , Huang Shipeng , Shi Wenguang , Liang Mengdi , Lin Yang , Shen Jie , Sui Minghao , Li Xuyang , Lai Qiliang , Dou Jie , Ge Yun , Zheng Min , Shao Zongze , Luo Xiaozhou 
Published: May 2023

Abstract
The Tistrella genus can produce the cyclic depsipeptide didemnin B, but the biosynthetic origin of its analogue, plitidepsin, remains unknown. Plitidepsin is an approved anticancer drug and an anti-COVID-19 agent in phase III clinical trials. Here, we employed a novel approach that combined microbial and chemical synthesis to produce plitidepsin. We first investigated the global distribution of Tistrella, which inspired us to retrieve and screen a library of Tistrella. Using a genetic approach and heterologous expression, we, for the first time, experimentally confirmed the biosynthetic gene cluster (BGC) for didemnins. After duplication of the BGC, we obtained a yield of didemnin B reached to 70 mg/L in the engineered strain. We then developed two chemical strategies to convert didemnin B to plitidepsin, one of which resulted in over 90% overall yield in a one-step synthetic route. Additionally, we synthesized two new didemnin analogues and assessed their anticancer and antiviral activities. Our study provides a practical and sustainable solution for producing plitidepsin and its derivatives, which may expedite didemnin drug development.
Ebelin lactone as the most promising neuroprotective compound from Bacopa monnieri extract targeting microtubule affinity regulation kinase-4 involved in Alzheimer’s disease: A Computational Study
Authors: Ahmad Faizan , Abiha Umme , Ahmad S Rehan , Patel Nandan 
Published: May 2023

Abstract
Background Alzheimer's disease (AD) is a progressive neurodegenerative disorder in which amyloid-beta (Aβ) and tau tangles are vital in causing neurodegeneration. Only five FDA-approved drugs are available in the market which manages the symptoms. In this study, 52 novel phytochemicals were selected from the medicinal plant Bacopa monnieri, a medicinal plant with neuroprotective compounds.Objective The main aim of this study is to find the most promising compound inhibiting microtubule affinity regulation kinase 4 (MARK 4), which is involved in AD.Methods First, an ADMET analysis was conducted, and the selected compounds were molecularly docked against the MARK4-associated protein (5ES1). Based on the top five binding affinities, a molecular dynamics simulation was performed.Results Cucurbitacin E, oroxindin, ebelin lactone, cucurbitacin B, and bacosine showed binding affinity of more than − 10.0 kcal/mol, and molecular dynamics (MD) simulations of these molecules in complex with MARK4 was performed. Subsequent trajectory analysis for structural changes and end-state MMGBSA binding energy were performed for Cucurbitacin E, oroxindin, ebelin lactone, cucurbitacin B and bacosine. The MD simulation and MMGBSA calculations showed stable interactions between the screened molecules and MARK4.Conclusion This computational study predicted ebelin lactone to be the most promising compound from Bacopa monnieri that can be further developed as a drug to treat AD after pre-clinical and clinical studies.
LAMC2 promotes EGFR cell membrane localization and acts as a novel biomarker for tyrosine kinase inhibitors (TKIs) sensitivity in lung cancer
Authors: Huang Chen , Tong Dongdong , Wang Xiaofei , Liu Liying , Wen Ting , Chen QiaoYi 
Published: May 2023

Abstract
The epidermal growth factor receptor (EGFR) is one of the first and most prominent driver genes known to promote malignant lung cancer. Investigating regulatory mechanisms beyond ligand-receptor binding, phosphorylation, and receptor kinase activation as means of EGFR signaling activation is important for improving EGFR-targeted therapy. Here, we report that Laminin-5γ-2 (LAMC2) retained high oncogenic capacity in lung cancer, silencing LAMC2 inhibited EGFR-induced cell proliferation and tumor growth in vivo. Deletion mutation experiments showed that both the EGF-Lam and LamB regions of LAMC2 are necessary for EGFR receptor binding, and that LAMC2 and EGFR were found to co-localize at the endoplasmic reticulum (ER) membrane. In addition, LAMC2 overexpression enhanced EGFR membrane deposition and promoted EGFR transport from the ER. Moreover, LAMC2 was necessary for preventing EGFR protein degradation via ubiquitination. Lastly, our study showed that high LAMC2 expression is positively associated with response to Gefitinib (EGFR tyrosine kinase inhibitor) treatment. Overall, our study revealed a new regulatory mechanism of LAMC2 in promoting EGFR protein expression and stability by facilitating ER transport and preventing protein degradation via ubiquitination. Moreover, LAMC2 may serve as a stratifying biomarker for patients suitable for EGFR-TKI treatment.
Dementia and cognitive impairment in French-speaking Sub-Saharan Africa: a comprehensive review on moving out of the shadows of neglect
Authors: Nkouonlack Cyrille D. , Njamnshi Wepnyu Y. , Angwafor Samuel A. , Fodjo Joseph N. Siewe , Mengnjo Michel K. , Ngarka Leonard , Mbede Maggy , Nfor Leonard N. , Abomate Carlson , Nganchfu Kevin , Ahidjo Nene , Atchou Jonas G. Basseguin , Mendo Edwige L. , Esene Ignatius , Mbong Eta N. , Bila Eric G. , Nchufor Roland N. , Vechio Lorella , Mouofor Edmond N. , Belinga Luc N. , Fonsah Julius Y. , Nsoh Edith N. , Dema Fidele , Chokote Eric S. , Tabah Earnest N. , Kenmogne Caroline , Wadop Yannick N. , Kiatoko Nono Ponte , Sakadi Fouksona , Epenge Emmanuel , Djibo Fatimata H. , Dardar Ibrahim , Kapto Olivier , Tatah Godwin Y. , Ongolo-Zogo Pierre , Nsagha Dickson S. , Houesso Mendinatou A. , Diallo Seybou H. , Diarra Evelyne , Ayele Biniyam A. , Mpandzou GA. , Sounga Eliot , Ossou-Nguiet Paul M. , Maiga Yousoufa , Napon Christian , Mbelesso Pascal , Guerchet Maelenn , Toure Kamadore , Koume-Assouan Ange-Eric , Tehindrazanarivelo Alain D. , Kouna Philomene , Tchoua Roman , Houinatou Disman , Millogo Athanase , Kissani Najib , Luabeya Mesua K. , Balugou Agnon A.K. , Nditei David , Beugre Kouassi , Richetin Kevin , Vulliemoz Serge , Perrig Stephen , Chabwine Joelle N. , Sztajzel Roman , Etya'ale Daniel , Annoni Jean Marie , Etet Paul F. Seke , Ihunwo Amadi O. , Leke Rose G.F. , Ondoa Marie Therese Obama , Cavazos Jose E. , Maestre Gladys , Seshadri Sudha , Fongang Bernard , Njamnshi Alfred K. 
Published: May 2023

AbstractDementia is a global public health problem with increasing prevalence and incidence worldwide. The African continent is expected to bear the biggest brunt of the burden of dementia by 2050 because of the rapid demographic changes, including rapid population growth, an increase in life expectancy, and ageing. However, French-speaking Sub-Saharan African (FS-SSA) countries are underrepresented in research on dementia in Africa. While the reasons are diverse and complex, linguistic and cultural barriers to research, disproportionately affect these countries and may be significant factors. Any efforts, therefore, to redress the burden of dementia in Africa must consider the specific demographic, cultural, and linguistic characteristics of FS-SSA countries. This scoping review explores the current state of knowledge in dementia and cognitive impairment in Sub-Saharan Africa, highlighting research gaps and specific patterns unique to FS-SSA Africa. We identify pathways for research to bridge the knowledge gaps on dementia in FS-SSA as part of the global endeavor to tackle dementia worldwide.
3D Printing rGO/PCL/Mel Bionic Conduit for Peripheral Nerve Regeneration
Authors: Zhang Qiangqiang , Zhang Jingxiang , Liu Zhongyang , Wang Jing , Zhang Yang , Dong Jiaqi , Gao Jianpeng , Zhang Licheng , Tang Peifu , Wang Jizeng 
Published: May 2023

Abstract
The inevitable secondary victimization of patients during the grafting of autogenous nerve necessitates the urgent development of bioactive conduits for the precise repair of peripheral nerve (PN) defects. However, the limited selection of appropriate components and inferior structural designs of many porous scaffolds have hindered satisfactory PN regeneration. In this study, we created a 3D hollow conduit of reduced graphene oxide (rGO) with a hierarchically ordered microstructure through a coaxial printing methodology that enabled a physicochemically cooperative construction process at multiscale. We deposited a mixture of polycaprolactone (PCL) and melatonin (Mel) as the biologically enhancing constitution conformably over the 3D rGO templated conduit. Attributing to its elaborately designed hierarchical structure and arched alignment of 2D micro sheets, the 3D rGO/PCL/Mel hybrid bio-conduit has demonstrated remarkable structural robustness in maintaining ordered pathways and high porosity (98.5 ± 0.24%), which facilitated nerve growth in a complex survival environment in vivo. Furthermore, the excellent combination of properties such as electrical conductivity, biocompatibility, and mechanical properties (with an elastic modulus ranging from 7.06 ± 0.81 MPa to 26.58 ± 4.99 MPa), has led to highly efficient regeneration of well-ordered PN tissue. Systematic evaluations of nerve regeneration and muscle function recovery in an SD rat model with a long nerve defect (> 15 mm) have validated the virtually identical performance of the 3D rGO/PCL/Mel conduit compared to the autogenous nerve graft group. This study confirms a promising approach to clinical PN repair of long defects through the combined regulation of rational structure design on multiscale and indispensable chemical modification of rGO-based functional nerve regeneration conduits.
Complications from the formation of preventive stomas in the surgical treatment of rectal cancer
Authors: Aliev I. I. , Smirnov A. A. , Pavlov R. V. , Komyak K. N. , Ivlev D. A. , Domanskiy N. A. , Sharygin G. A. , Nazmiev A. I. 
Published: May 2023

Background. Anastomotic leakage is one of the most severe complications of colorectal cancer surgery. The formation of a preventive stoma can avoid the consequences of this complication. Given the increase in the rate of sphincter-preserving surgeries, the number of surgeries with the formation of preventive stoma has also increased significantly. There are several techniques and each of them has its advantages and disadvantages.The aim of the study was to compare the effectiveness of surgical treatment and techniques of preventive stoma formation.Material and Methods. The retrospective study included 353 patients with stage II–IV rectal cancer who underwent surgery with the formation of preventive stoma (110 with ileostomy formation, 243 with transversostomy) from 2016 to 2020. Both intraoperative parameters (operation time, blood loss, intraoperative complications) and postoperative parameters (postoperative bed-day, complications) were evaluated. Immediate complications within 30 days after surgery using the Clavien–Dindo complication scale and infectious complications mainly related to the stoma formation (prolapse, stoma retraction, presence of peristomal dermatitis, reoperation) were assessed.Conclusion. The choice in the formation of a preventive ileo- or transversostomy during rectal resection has no effect on the frequency of anastomosis failure; however, there is a tendency that patients with colostomy spend fewer bed days in a hospital. Stoma-related complications in the postoperative period are mild and can be treated conservatively in the vast majority of cases. In compliance with the standardized surgical procedures and the protocol of perioperative management of patients, the number of severe complications is minimal. It is necessary to evaluate the second step of management of patients with preventive stomas – their elimination.
Expression of LAG-3 on B-lymphocytes as a marker for prediction of response to therapy in patients with chronic lymphocytic leukemia
Authors: Selyutina O. N. , Lysenko I. B. , Guskova N. K. , Novikova I. A. , Zlatnik E. Yu. , Pushkareva T. F. , Nikolaeva N. V. , Kamaeva I. A. , Samaneva N. Yu. , Kapuza E. A. 
Published: May 2023

Purpose: to study the level of LAG-3 expression on B-lymphocytes and the feasibility of using it as a marker for predicting response to therapy in patients with chronic lymphocytic leukemia (CLL).Material and Methods. The study included 40 patients with newly diagnosed CLL. All patients were divided into two groups: group I: patients with Binet stage A, who did not receive therapy and group II: patients with Binet stage C, who received immunochemotherapy in RB and FCR regimes. According to the treatment regimen and hematological response to therapy, 4 subgroups were distinguished: IIA-RB, IIA-FCR, IIB-RB, and IIB-FCR. The control group consisted of 20 people matched in age and gender without cancer. The immunophenotype, level of B-lymphocytes, LAG-3 expression, and the minimal residual disease in group II after the 6th course of immunochemotherapy were initially determined in all groups by flow cytometry. The data were evaluated using Statistica 13.0.Results. Compared to the control group, the LAG-3 expression on B-lymphocytes was found in all groups of CLL patients before treatment. The expression level was higher in patients with Binet stage C than in patients with Binet stage. The data demonstrated differences in the level of LAG-3 expression in patients with different hematological responses to therapy. The initially higher level of LAG-3 expression on B-lymphocytes was observed in patients with Binet stage C CLL with an unfavorable response to therapy. A good hematological response was found can be achieved at the level of LAG-3 expression within 14.57 ± 0.66 % regardless of the therapy regimen, and unfavorable response to therapy at the level of 41.95 ± 1.62 %.Conclusion. The initial level of LAG-3 expression on B-lymphocytes in patients with CLL can be used as a marker for predicting and monitoring response to treatment, regardless of the immunochemotherapy regimen used.
Knockout of the histone methyltransferase NSD1 leads to a decrease in cell proliferation and an increase in sensitivity to cisplatin in laryngeal squamous cell carcinoma
Authors: Topchu I. A. , Tikhomirova M. V. , Bulatov E. R. , Abramova Z. I. , Boumber Y. A. 
Published: May 2023

Introduction. The histone methylation regulates gene expression and plays a role in genomic stability participating in DNA repair. Dimethylation of histone 3 lysine 36 (H3K36me2) is an important histone modification which is responsible for gene expression activation. H3K36me2 is a product of methyltransferase activity of NSD1, NSD2, NSD3, and ASH1L proteins. NSD1 mutations are known to often occur in head and neck squamous carcinoma. The presence of NSD1 mutations highly correlates with increased survival, especially for patients with laryngeal cancer. The aim of this study was an in vitro investigation of the role of NSD1 in the cell proliferation of laryngeal squamous cell cancer and non-small lung cancer cells, as well as a study of the effect of disruption of the NSD1 gene expression on cisplatin treatment response.Material and Methods. Using TCGA, correlation analysis was performed to compare NSD1 wild type and mutant patient survival. NSD1 knockout cell lines models of laryngeal and non-small cell lung cancer were developed using the CRISPR/ Cas9 system. The effect of NSD1 knockout on H3K36me2 level was evaluated by western blot. Proliferation and IC50 of cisplatin in control and knockout cells were studied as well.Results. It was demonstrated that NSD1 knockout decreased the H3K36me2 level and cell proliferation in laryngeal squamous cell cancer cells and increased the sensitivity of head and neck cancer cells to cisplatin treatment, while there was no effect of NSD1 knockout in a non-small cell lung cancer cell line.Conclusion. Based on the data obtained, it can be concluded that the NSD1 protein is a potential target for inhibitor development following in vitro and in vivo testing in head-neck squamous cell carcinoma models. More studies are needed for better understanding of the regulation of tumor cell growth by NSD1.
Approximation of aeration efficiency at sharp-crested weirs using metaheuristic regression approaches
Authors: Jaiswal Akash , Goel Arun , Sihag Parveen 
Published: May 2023

Abstract

This paper explores the ability of multivariate adaptive regression splines, decision trees, Gaussian processes, and multiple non-linear regression equation approaches to predict the aeration efficiency at various weirs and discusses their results. In total, 126 experimental observations were collected in the laboratory, of which 88 were arbitrarily selected for model training, and the rest were used for model validation. Various graphical presentations and goodness-of-fit parameters were used to assess the performance of the models. Performance evaluation results, Whisker plot, and Taylor's diagram indicated that the GP_rbf-based model was superior to other implemented models in predicting the aeration efficiency of weirs with CC (0.9961 and 0.9973), MAE (0.0079 and 0.0195), RMSE (0.0122 and 0.0251), scattering index (0.0594 and 0.1238), and Nash Sutcliffe model efficiency (0.9923 and 0.9564) values in the training and validating stages, respectively. The predicted values by GP_rbf lie within the ±30% error line in the training and validating stages, with most of it lying at/close to the line of agreement. The random forest model had better predictability than other decision tree models implied. The sensitivity analysis of parameters suggests shape factor and drop height as major influencing factors in predicting the aeration efficiency.