bluuebunny/crossref_metadata_2025_split huggingface dataset:
Regenerative agriculture v. conservation agriculture: potential effects on soil quality, crop productivity and whole-farm economics in Mediterranean-climate regions
Authors: Musto G. A. , Swanepoel P. A. , Strauss J. A. 
Published: April 2023

AbstractSmall-grain farming systems in Mediterranean climatic regions are characterized by poor quality soils, high climate variability, and resulting heavy agrochemical reliance. The adoption of Conservation Agriculture, based on minimum tillage, permanent soil cover, and crop rotations, has improved soil quality, enhanced crop productivity, and help mitigate financial risk. However, emerging issues that threaten sustainability, such as herbicide resistance, inputs costs rising disproportionately to product prices, and increasing climate variability and unpredictability, drive the need for innovation in small-grain Mediterranean-based CA systems. The aim of the review was to evaluate a set of agroecological practices which constitute a Regenerative Agriculture (RA) concept, for their potential to address these challenges from a soil quality, crop productivity and whole-farm economics perspective. Organic soil amendments derived from agro-wastes, offer promising perspectives for supplying appropriate quantities of nutrients to reduce or replace mineral fertilisers and offset their economic and agroecological costs. Although the viability of microbial bioeffectors in small-grain agroecosystems was largely under question, non-microbial bioeffectors and certain combination options represent more efficient and cost-effective uses of this technology. Their widely reported abiotic stress priming functions and crop productivity enhancement under poor growing conditions may improve yield stability and financial resilience in small-grain Mediterranean CA systems. Finally, multi-species cover crops subjected to adaptive multi-paddock (AMP) grazing as a phase within the crop rotation may reduce weed pressure, enhance soil multi-functionality, and resilience against environmental stresses. To validate the potential of these technologies in Mediterranean small-grain systems, more long-term and context-specific research is called for.
Options Trading and Stock Price Informativeness
Authors: Cao Jie , Goyal Amit , Ke Sai , Zhan Xintong 
Published: April 2023

AbstractWe document the causal effects of single-name options trading on the absolute level of information content of prices (stock price informativeness) by exploiting the Penny Pilot Program as an exogenous shock to options trading volume. We find that options trading increases underlying stock price informativeness and information acquisition by both option and stock investors, consistent with the framework of Goldstein and Yang (2015). The findings are driven by firms for which options are more important sources of information and firms with more efficiently priced options. Options market introduction in a sample of 25 other economies also leads to higher price informativeness.
Cochlear implantation in advanced otosclerosis: utility of pre-operative radiological assessment in predicting intra-operative difficulty and final electrode position
Authors: Wong D , Copson B , Gerard J-M , Hill F , Leigh J , Dowell R 
Published: April 2023

AbstractObjectiveThis study aimed to determine if pre-operative radiological scoring can reliably predict intra-operative difficulty and final cochlear electrode position in patients with advanced otosclerosis.MethodA retrospective cohort study of advanced otosclerosis patients who underwent cochlear implantation (n = 48, 52 ears) was compared with a larger cohort of post-lingually deaf adult patients (n = 1414) with bilateral hearing loss and normal cochlear anatomy. Pre-operative imaging for advanced otosclerosis patients and final electrode position were scored and correlated with intra-operative difficulty and speech outcomes.ResultsAdvanced otosclerosis patients benefit significantly from cochlear implantation. Mean duration of deafness was longer in the advanced otosclerosis group (19.5 vs 14.3 years; p < 0.05).ConclusionAnatomical changes in advanced otosclerosis can result in increased difficulty of surgery. Evidence of pre-operative cochlear luminal changes was associated with intra-operative difficult insertion and final non-scala tympani position. Nearly all electrodes implanted in the advanced otosclerosis cohort were peri-modiolar. No reports of facial nerve stimulation were observed.
Autonomous social robots are real in the mind's eye of many
Authors: Caruana Nathan , Cross Emily S. 
Published: April 2023

Abstract
Clark and Fischer's dismissal of extant human–robot interaction research approaches limits opportunities to understand major variables shaping people's engagement with social robots. Instead, this endeavour categorically requires multidisciplinary approaches. We refute the assumption that people cannot (correctly or incorrectly) represent robots as autonomous social agents. This contradicts available empirical evidence, and will become increasingly tenuous as robot automation improves.
Increase of Simultaneous Soybean Failures Due To Climate Change
Authors: Goulart Henrique M. D. , van der Wiel Karin , Folberth Christian , Boere Esther , van den Hurk Bart 
Published: April 2023

AbstractWhile soybeans are among the most consumed crops in the world, most of its production lies in the US, Brazil, and Argentina. The concentration of soybean growing regions in the Americas renders the supply chain vulnerable to regional disruptions. In 2012, anomalous hot and dry conditions occurring simultaneously in these regions led to low soybean yields, which drove global soybean prices to all‐time records. In this study, we explore climate change impacts on simultaneous extreme crop failures as the one from 2012. We develop a hybrid model, coupling a process‐based crop model with a machine learning model, to improve the simulation of soybean production. We assess the frequency and magnitude of events with similar or higher impacts than 2012 under different future scenarios, evaluating anomalies both with respect to present day and future conditions to disentangle the impacts of (changing) climate variability from the long‐term mean trends. We find long‐term trends in mean climate increase the frequency of 2012 analogs by 11–16 times and the magnitude by 4–15% compared to changes in climate variability only depending on the global climate scenario. Conversely, anomalies like the 2012 event due to changes in climate variability show an increase in frequency in each country individually, but not simultaneously across the Americas. We deduce that adaptation of the crop production practice to the long‐term mean trends of climate change may considerably reduce the future risk of simultaneous soybean losses across the Americas.
GOLD Observations of Longitudinal Variations in the Nighttime Equatorial Ionization Anomaly (EIA) Crests' Latitudes
Authors: Eastes R. W. , Karan D. K. , Martinis C. , Daniell R. E. , Gan Q. , Burns A. G. , McClintock W. E. 
Published: April 2023

AbstractEach day the Global‐scale Observations of the Limb and Disk imager observes the equatorial ionization anomaly (EIA) near sunset from ∼10°E to ∼80°W geographic longitude. Most images cover ∼45° of longitude (∼3 hr), and most longitudes are observed multiple times. Monthly averages of EIA crests' latitude (EIA lats) versus longitude during March, September, and December 2020 have been analyzed. The EIA lats reflect the combined influence of winds, solar radiation, and fields (electric and magnetic) in the equatorial region. Winter solstice differs significantly from the equinoxes, which are similar, but there are notable similarities between all three. The similarities in the EIA lats during the seasons examined indicates that the magnetic equator to subsolar point separation influences them in all three seasons and that it has a more distinct, possibly more significant, influence than winds on the average latitudes.
The Role of Equatorially Forced Waves in Triggering Benguela Niño/Niña as Investigated by an Energy Flux Diagnosis
Authors: Song Qingyang , Aiki Hidenori , Tang Youmin 
Published: April 2023

AbstractThe Benguela Niño/Niña events manifest the anomalous signals of sea surface temperature (SST) in the coastal region of the tropical Atlantic Ocean. These events are triggered by the interannual modulation of either equatorial waves or local atmospheric forcing. In the present study, the mechanism that equatorial waves induce the coastal SST anomaly is investigated in terms of the transfer episodes of wave energy. The analysis suggests that most of the coastal events can be reproduced by subseasonal wind forcing with an interannual modulation that excites oceanic waves of the first three baroclinic modes. The transfer routes of wave energy illustrate the role of wave dynamics that explains how the interannual variability of SST in the equatorial Atlantic is connected with that in coastal regions. The linearly superposed sign‐indefinite potential energy flux owing to waves manifests its capability of sufficiently displacing the thermocline so as to trigger the coastal events. The results suggest that the equatorial wave signal is able to provide credible predictions for remotely forced Benguela Niño/Niña events if a proper transection in central equatorial Atlantic with an appropriate threshold is selected for observing.
The Climatology of Elevated Stratopause Events in the UA‐ICON Model and the Contribution of Gravity Waves
Authors: Karami Khalil , Borchert Sebastian , Eichinger Roland , Jacobi Christoph , Kuchar Ales , Mehrdad Sina , Pisoft Petr , Sacha Petr 
Published: April 2023

AbstractThe climatologies of the stratopause height and temperature in the UA‐ICON model are examined by comparing them to 17‐years (2005–2021) of Microwave Limb Sounder (MLS) observations. In addition, the elevated stratopause (ES) event occurrence, their main characteristics, and driving mechanisms in the UA‐ICON model are examined using three 30‐year time‐slice experiments. While UA‐ICON reasonably simulates the large‐scale stratopause properties similar to MLS observations, at polar latitudes in the Southern Hemisphere the stratopause is ∼8 K warmer and ∼3 km higher than observed. A time lag of about two months also exists in the occurrence of the tropical semiannual oscillation of the stratopause compared to the observations. ES events occur in ∼20% of the boreal winters, after major sudden stratospheric warmings (SSWs). Compared to the SSWs not followed by ES events (SSW‐only), the ES events are associated with the persistent tropospheric forcing and prolonged anomalies of the stratospheric jet. Our modeling results suggest that the contributions of both gravity waves (GW)s and resolved waves are important in explaining the enhanced residual circulation following ES events compared to the SSW‐only events but their contributions vary through the lifetime of ES events. We emphasize the role of the resolved wave drag in the ES formation as in the sensitivity test when the non‐orographic GW drag is absent, the anomalously enhanced resolved wave forcing in the mesosphere gives rise to the formation of the elevated stratopause at about 85 km.
110 μm thin endo-microscope for deep-brain in vivo observations of neuronal connectivity, activity and blood flow dynamics
Authors: Stibůrek Miroslav , Ondráčková Petra , Tučková Tereza , Turtaev Sergey , Šiler Martin , Pikálek Tomáš , Jákl Petr , Gomes André , Krejčí Jana , Kolbábková Petra , Uhlířová Hana , Čižmár Tomáš 
Published: April 2023

AbstractLight-based in-vivo brain imaging relies on light transport over large distances of highly scattering tissues. Scattering gradually reduces imaging contrast and resolution, making it difficult to reach structures at greater depths even with the use of multiphoton techniques. To reach deeper, minimally invasive endo-microscopy techniques have been established. These most commonly exploit graded-index rod lenses and enable a variety of modalities in head-fixed and freely moving animals. A recently proposed alternative is the use of holographic control of light transport through multimode optical fibres promising much less traumatic application and superior imaging performance. We present a 110 μm thin laser-scanning endo-microscope based on this prospect, enabling in-vivo volumetric imaging throughout the whole depth of the mouse brain. The instrument is equipped with multi-wavelength detection and three-dimensional random access options, and it performs at lateral resolution below 1 μm. We showcase various modes of its application through the observations of fluorescently labelled neurones, their processes and blood vessels. Finally, we demonstrate how to exploit the instrument to monitor calcium signalling of neurones and to measure blood flow velocity in individual vessels at high speeds.
Oncogenic CALR mutant C-terminus mediates dual binding to the thrombopoietin receptor triggering complex dimerization and activation
Authors: Papadopoulos Nicolas , Nédélec Audrey , Derenne Allison , Şulea Teodor Asvadur , Pecquet Christian , Chachoua Ilyas , Vertenoeil Gaëlle , Tilmant Thomas , Petrescu Andrei-Jose , Mazzucchelli Gabriel , Iorga Bogdan I. , Vertommen Didier , Constantinescu Stefan N. 
Published: April 2023

AbstractCalreticulin (CALR) frameshift mutations represent the second cause of myeloproliferative neoplasms (MPN). In healthy cells, CALR transiently and non-specifically interacts with immature N-glycosylated proteins through its N-terminal domain. Conversely, CALR frameshift mutants turn into rogue cytokines by stably and specifically interacting with the Thrombopoietin Receptor (TpoR), inducing its constitutive activation. Here, we identify the basis of the acquired specificity of CALR mutants for TpoR and define the mechanisms by which complex formation triggers TpoR dimerization and activation. Our work reveals that CALR mutant C-terminus unmasks CALR N-terminal domain, rendering it more accessible to bind immature N-glycans on TpoR. We further find that the basic mutant C-terminus is partially α-helical and define how its α-helical segment concomitantly binds acidic patches of TpoR extracellular domain and induces dimerization of both CALR mutant and TpoR. Finally, we propose a model of the tetrameric TpoR-CALR mutant complex and identify potentially targetable sites.
An α-chloroaldehyde-based formal synthesis of eribulin
Authors: Kaghad Anissa , Panagopoulos Dimitrios , Caballero-García Guillermo , Zhai Huimin , Britton Robert 
Published: April 2023

AbstractEribulin (Halaven) is the most structurally complex non-peptidic drug made by total synthesis and has challenged preconceptions of synthetic feasibility in drug discovery and development. However, despite decades of research, the synthesis and manufacture of eribulin remains a daunting task. Here, we report syntheses of the most complex fragment of eribulin (C14–C35) used in two distinct industrial routes to this important anticancer drug. Our convergent strategy relies on a doubly diastereoselective Corey–Chaykovsky reaction to affect the union of two tetrahydrofuran-containing subunits. Notably, this process relies exclusively on enantiomerically enriched α-chloroaldehydes as building blocks for constructing the three densely functionalized oxygen heterocycles found in the C14–C35 fragment and all associated stereocenters. Overall, eribulin can now be produced in a total of 52 steps, which is a significant reduction from that reported in both academic and industrial syntheses.
Astrocytic chloride is brain state dependent and modulates inhibitory neurotransmission in mice
Authors: Untiet Verena , Beinlich Felix R. M. , Kusk Peter , Kang Ning , Ladrón-de-Guevara Antonio , Song Wei , Kjaerby Celia , Andersen Mie , Hauglund Natalie , Bojarowska Zuzanna , Sigurdsson Björn , Deng Saiyue , Hirase Hajime , Petersen Nicolas C. , Verkhratsky Alexei , Nedergaard Maiken 
Published: April 2023

AbstractInformation transfer within neuronal circuits depends on the balance and recurrent activity of excitatory and inhibitory neurotransmission. Chloride (Cl−) is the major central nervous system (CNS) anion mediating inhibitory neurotransmission. Astrocytes are key homoeostatic glial cells populating the CNS, although the role of these cells in regulating excitatory-inhibitory balance remains unexplored. Here we show that astrocytes act as a dynamic Cl− reservoir regulating Cl− homoeostasis in the CNS. We found that intracellular chloride concentration ([Cl−]i) in astrocytes is high and stable during sleep. In awake mice astrocytic [Cl−]i is lower and exhibits large fluctuation in response to both sensory input and motor activity. Optogenetic manipulation of astrocytic [Cl−]i directly modulates neuronal activity during locomotion or whisker stimulation. Astrocytes thus serve as a dynamic source of extracellular Cl− available for GABAergic transmission in awake mice, which represents a mechanism for modulation of the inhibitory tone during sustained neuronal activity.
The F-pilus biomechanical adaptability accelerates conjugative dissemination of antimicrobial resistance and biofilm formation
Authors: Patkowski Jonasz B. , Dahlberg Tobias , Amin Himani , Gahlot Dharmender K. , Vijayrajratnam Sukhithasri , Vogel Joseph P. , Francis Matthew S. , Baker Joseph L. , Andersson Magnus , Costa Tiago R. D. 
Published: April 2023

AbstractConjugation is used by bacteria to propagate antimicrobial resistance (AMR) in the environment. Central to this process are widespread conjugative F-pili that establish the connection between donor and recipient cells, thereby facilitating the spread of IncF plasmids among enteropathogenic bacteria. Here, we show that the F-pilus is highly flexible but robust at the same time, properties that increase its resistance to thermochemical and mechanical stresses. By a combination of biophysical and molecular dynamics methods, we establish that the presence of phosphatidylglycerol molecules in the F-pilus contributes to the structural stability of the polymer. Moreover, this structural stability is important for successful delivery of DNA during conjugation and facilitates rapid formation of biofilms in harsh environmental conditions. Thus, our work highlights the importance of F-pilus structural adaptations for the efficient spread of AMR genes in a bacterial population and for the formation of biofilms that protect against the action of antibiotics.
Lateral septum adenosine A2A receptors control stress-induced depressive-like behaviors via signaling to the hypothalamus and habenula
Authors: Wang Muran , Li Peijun , Li Zewen , da Silva Beatriz S. , Zheng Wu , Xiang Zhenghua , He Yan , Xu Tao , Cordeiro Cristina , Deng Lu , Dai Yuwei , Ye Mengqian , Lin Zhiqing , Zhou Jianhong , Zhou Xuzhao , Ye Fenfen , Cunha Rodrigo A. , Chen Jiangfan , Guo Wei 
Published: April 2023

AbstractMajor depressive disorder ranks as a major burden of disease worldwide, yet the current antidepressant medications are limited by frequent non-responsiveness and significant side effects. The lateral septum (LS) is thought to control of depression, however, the cellular and circuit substrates are largely unknown. Here, we identified a subpopulation of LS GABAergic adenosine A2A receptors (A2AR)-positive neurons mediating depressive symptoms via direct projects to the lateral habenula (LHb) and the dorsomedial hypothalamus (DMH). Activation of A2AR in the LS augmented the spiking frequency of A2AR-positive neurons leading to a decreased activation of surrounding neurons and the bi-directional manipulation of LS-A2AR activity demonstrated that LS-A2ARs are necessary and sufficient to trigger depressive phenotypes. Thus, the optogenetic modulation (stimulation or inhibition) of LS-A2AR-positive neuronal activity or LS-A2AR-positive neurons projection terminals to the LHb or DMH, phenocopied depressive behaviors. Moreover, A2AR are upregulated in the LS in two male mouse models of repeated stress-induced depression. This identification that aberrantly increased A2AR signaling in the LS is a critical upstream regulator of repeated stress-induced depressive-like behaviors provides a neurophysiological and circuit-based justification of the antidepressant potential of A2AR antagonists, prompting their clinical translation.
Switchable biomimetic nanochannels for on-demand SO2 detection by light-controlled photochromism
Authors: Zhang Dan , Sun Yongjie , Wang Zhichao , Liu Fang , Zhang Xuanjun 
Published: April 2023

AbstractIn contrast to the conventional passive reaction to analytes, here, we create a proof-of-concept nanochannel system capable of on-demand recognition of the target to achieve an unbiased response. Inspired by light-activatable biological channelrhodopsin-2, photochromic spiropyran/anodic aluminium oxide nanochannel sensors are constructed to realize a light-controlled inert/active-switchable response to SO2 by ionic transport behaviour. We find that light can finely regulate the reactivity of the nanochannels for the on-demand detection of SO2. Pristine spiropyran/anodic aluminium oxide nanochannels are not reactive to SO2. After ultraviolet irradiation of the nanochannels, spiropyran isomerizes to merocyanine with a carbon‒carbon double bond nucleophilic site, which can react with SO2 to generate a new hydrophilic adduct. Benefiting from increasing asymmetric wettability, the proposed device exhibits a robust photoactivated detection performance in SO2 detection in the range from 10 nM to 1 mM achieved by monitoring the rectified current.
A severe neurodegenerative disease with Lewy bodies and a mutation in the glucocerebrosidase gene
Authors: Sipilä Jussi O. T. , Kytövuori Laura , Rauramaa Tuomas , Rauhamaa Hugo , Kaasinen Valtteri , Majamaa Kari 
Published: April 2023

AbstractSeveral heterozygous variants of the glucocerebrosidase gene (GBA1) have been reported to increase the risk of Parkinson’s disease (PD) and dementia with Lewy bodies (DLB). GBA1-associated PD has been reported to be more severe than idiopathic PD, and more deleterious variants are associated with more severe clinical phenotypes. We report a family with a heterozygous p.Pro454Leu variant in GBA1. The variant was associated with a severe and rapidly progressive neurodegenerative disease with Lewy bodies that were clinically and pathologically diverse. Pathogenicity prediction algorithms and evolutionary analyses suggested that p.Pro454Leu is deleterious.
Experimental analysis on cyanide removal of gold tailings under medium-temperature roasting
Authors: Hai Long , Fang Xianglong , Zhao Xin , Xu Bo , Cheng Tongjun 
Published: April 2023

AbstractThe cyanide content of gold tailings exceeds the standard seriously due to the cyanide extraction process. In order to improve the resource utilization efficiency of gold tailings, a medium-temperature roasting experiment was carried out on the stock tailings of Paishanlou gold mine after washing and pressing filtration treatment. The thermal decomposition rule of cyanide in gold tailings was analyzed, and the effects of different roasting temperatures and roasting durations on cyanide removal efficiency were compared. The results show that when the roasting temperature reaches 150 °C, the weak cyanide compound and free cyanide in the tailings begin to decompose. When the calcination temperature reached 300 °C, the complex cyanide compound began to decompose. When the roasting temperature reaches the initial temperature of cyanide decomposition, the cyanide removal efficiency can be improved by prolonging the roasting time. After roasting at 250–300 °C for 30–40 min, the total cyanide content in the toxic leachate decreased from 3.27 to 0.01 mg/L, which met the water quality standard of III class in China. The research results provide a low-cost and efficient way for cyanide treatment, which is of great significance for promoting the resource utilization of gold tailings and other cyanide-containing wastes.