bluuebunny/crossref_metadata_2025_split huggingface dataset:
Infectious clone of a contemporary Tembusu virus and replicons expressing reporter genes or heterologous antigens from poultry viruses
Authors: Comes Jerome D. G. , Poniman Meliawati , van Oosten Linda , Doets Kristel , de Cloe Sjoerd , Geertsema Corinne , Pijlman Gorben P. 
Published: September 2023

AbstractThe novel mosquito‐borne Tembusu virus (TMUV, family Flaviviridae) was discovered as the cause of a severe outbreak of egg‐drop syndrome affecting ducks in Southeast Asia in 2010. TMUV infection can also lead to high mortality in various additional avian species such as geese, pigeons, and chickens. This study describes the construction of an infectious cDNA clone of a contemporary duck‐isolate (TMUV WU2016). The virus recovered after transfection of BHK‐21 cells shows enhanced virus replication compared to the mosquito‐derived MM1775 strain. Next, the WU2016 cDNA clone was modified to create a SP6 promoter‐driven, self‐amplifying mRNA (replicon) capable of expressing a range of different reporter genes (Renilla luciferase, mScarlet, mCherry, and GFP) and viral (glyco)proteins of avian influenza virus (AIV; family Orthomyxoviridae), infectious bursal disease virus (IDBV; family Bunyaviridae) and infectious bronchitis virus (IBV; family Coronaviridae). The current study demonstrates the flexibility of the TMUV replicon system, to produce different heterologous proteins over an extended period of time and its potential use as a platform technology for novel poultry vaccines.
Mature teratoma of conus medullaris: A case report and review of literature
Authors: Eslami Masoud , Ilaghi Mehran , Shahabinejad Erfan , Khajepour Forouzande , Karamouzian Saeed , Reihani‐Kermani Hamed 
Published: September 2023

Key Clinical MessageIn conus medullaris, mature teratomas are rare. We report a case of a 40‐year‐old man who presented with urinary incontinence, low back pain, and muscle weakness. Magnetic resonance imaging revealed a mass in conus medullaris (T11–L1), further confirmed as a mature teratoma by pathological examination. We identified 63 cases of conus medullaris teratoma over the past two decades by systematically analyzing the case reports. Findings demonstrated that most cases were diagnosed in the fourth decade of life, with the majority of cases (57.6%) being male. Lower back pain, radiating pain in the extremities, hypoesthesia, and urinary dysfunction are the most common clinical presentations among patients with teratoma of conus medullaris. Mature teratoma is the dominant pathologic subtype of teratomas in this region, comprising more than 95% of cases. Our case highlights the importance of considering spinal teratoma as a differential diagnosis in patients presenting with urinary incontinence and lumbar pain.
Angiomyofibroblastoma as a rare cause of vulvar mass: A case report and literature review
Authors: Einafshar Negar , Shari’at Moghani Mahta , Radboy Mahsa , Zandbaf Tooraj 
Published: September 2023

Key Clinical MessageAngiomyofibroblastoma is a benign soft tissue tumor and a form of genital stromal mesenchymal tumor that primarily affects the vulva. It could possibly affect the reproductive‐aged women's lower genital tract (vagina).AbstractAngiomyofibroblastoma is a rare benign soft tissue tumor primarily affecting the vulva in reproductive‐aged women. We report a 67‐year‐old female complaining of a painless mass in her right vulva spreading to the right inguinal region over the past 2 years. The first clinical impression was a canal of Nuck hernia, diagnostic laparoscopy was planned to rule hernia out. The vulvar mass was excised, and a histopathology examination revealed Angiomyofibroblastoma.
Why DFT‐Based Tight Binding Gives a Better Representation of the Potential at Metal‐Solution Interfaces than DFT Does
Authors: Quaino Paola , Nuñez José Luis , Aradi Bálint , van der Heide Tammo , Santos Elizabeth , Schmickler Wolfgang 
Published: September 2023

AbstractIn modelling electrochemical interfaces it is important to treat electrode and electrolyte at the same level of theory. Density functional theory, which is usually the method of choice, suffers from a distinct disadvantage: The inner potential is calculated as the average of the total electrostatic potential. This includes the highly localized potential generated from the nuclei. The resulting inner potential is far too high, of the order of 3.5 V, and not relevant for electrochemistry. In the density functional based tight binding (DFTB) method the electrostatic potential is much smoother, as it stems from atomic charge fluctuations with respect to neutral reference atoms. The resulting values for the electrochemical inner potential are much lower and compare well with those obtained by other, elaborate methods. Thus DFTB recommends itself as a method for treating the electrochemical interface including the inner potential.
Fluorescence Enhancement of Adamantane‐Modified Dyes in Aqueous Solution via Supramolecular Interaction with Methyl‐β‐cyclodextrin and Their Application in Cell Imaging
Authors: Liu Hong‐Jiao , Zhang Gang , Xu Yu‐Jie , Sun Ru , Ge Jian‐Feng 
Published: September 2023

AbstractThe fluorescence of functional dyes was generally quenched in aqueous solution, which hindered their application in water‐bearing detections. In this work, a novel strategy based on host‐guest interaction was provided for the purpose of fluorescence enhancement in aqueous solution and cell imaging. Three adamantane‐modified fluorescent dyes (Coum‐Ad, NP‐Ad, NR‐Ad) with coumarin, 1,8‐naphthalimide and Nile Red as fluorophores were initially designed and prepared. The ((adamantan‐1‐yl)methyl)amino group, as the auxochrome of those dyes, complexed with methylated β‐cyclodextrin (M‐β‐CD) via supramolecular interaction, and then fluorescent supramolecular nanoparticles (FSNPs) were formed by self‐assembly in water. The inclusion equilibrium constant (K) could be as high as 3.94×104 M−1. With the addition of M‐β‐CD, fluorescence quantum yields of these dyes were separately improved to 69.8 %, 32.9 % and 41.3 %. Inspired by the above satisfactory results, six adamantane‐modified probes organelle‐NPAds with organelle‐targeting capability were further obtained. As the formation of hydrogen bonds between organelle‐NPAd2 and M‐β‐CD verified by theoretical calculation, K of organelle‐NPAd2 (5.13×104 M−1~4.53×105 M−1) with M‐β‐CD was higher than that of organelle‐NPAd1 (1.15×104 M−1~3.66×104 M−1) and their fluorescence quantum yields increased to 32.8 %~83.6 % in aqueous solution. In addition, fluorescence enhancement was realized in cell imaging with the addition of M‐β‐CD.
Construction and Hierarchical Self‐Assembly of a Supramolecular Metal–Carbene Complex with Multifunctional Units
Authors: Zhang Zi‐En , An Yuan‐Yuan , Wang Fang , Li Hui‐Ling , Jiang Wei‐Ling , Han Ying‐Feng 
Published: September 2023

AbstractHierarchical combinations involving metal–ligand interactions and host−guest interactions can consolidate building blocks with unique functions into material properties. This study reports the construction and hierarchical self‐assembly of multifunctional trinuclear AuI tricarbene complex containing three crown ether units and three ferrocene units. Host−guest interactions between the multifunctional trinuclear AuI tricarbene complex and organic ammonium salts were investigated, revealing that crown ether‐based host−guest interactions can effectively regulate the electrochemical properties of the complex. Utilizing bisammonium salt as the cross‐linker and multifunctional trinuclear AuI tricarbene complex as the core, a stimuli‐responsive and self‐healing supramolecular gel with different functional units was obtained.
Development and validation of a novel TNM staging N‐classification of oral cavity squamous cell carcinoma
Authors: de Almeida John R. , Su Jie Susie , Kolarski Mirko Manojlovic , Truong Tra , Weinreb Ilan , Perez‐Ordonez Bayardo , Smith Stephen M. , Hosni Ali , Patel Snehal , Valero Cristina , Xu Bin , Ghossein Ronald , Katabi Nora , Clark Jonathan , Low Tsu‐Hui (Hubert) , Gupta Ruta , Graboyes Evan M. , Davies Joel , Richardson Mary , Pasham Vishu , Jester Rachel , Goldstein David P. , Huang Shao Hui , Xu Wei , O’Sullivan Brian 
Published: September 2023

AbstractBackgroundFor oral cavity squamous cell carcinoma (OSCC), extent of extranodal extension (ENE) (minor, ≤2 mm; major, >2 mm) is differentially prognostic, whereas limitations exist with the 8th edition of American Joint Committee on Cancer/International Union Against Cancer TNM N‐classification (TNM‐8‐N).MethodsResected OSCC patients at four centers were included and extent of ENE was recorded. Thresholds for optimal overall survival (OS) discrimination of lymph node (LN) features were established. After dividing into training and validation sets, two new N‐classifications were created using 1) recursive partitioning analysis (RPA), and 2) adjusted hazard ratios (aHRs) and were ranked against TNM‐8‐N and two published proposals.ResultsA total of 1460 patients were included (pN0: 696; pN+: 764). Of the pN+ cases, 135 (18%) had bilateral/contralateral LNs; 126 (17%) and 244 (32%) had minor and major ENE, and two (0.3%) had LN(s) >6 cm without ENE (N3a). LN number (1 and >1 vs. 0: aHRs, 1.92 [95% confidence interval (CI), 1.44–2.55] and 3.21 [95% CI, 2.44–4.22]), size (>3 vs. ≤3 cm: aHR, 1.88 [95% CI, 1.44–2.45]), and ENE extent (major vs. minor: aHR, 1.40 [95% CI, 1.05–1.87]) were associated with OS, whereas presence of contralateral LNs was not (aHR, 1.05 [95% CI, 0.81–1.36]). The aHR proposal provided optimal performance with these changes to TNM‐8‐N: 1) stratification of ENE extent, 2) elimination of N2c and 6‐cm threshold, and 3) stratification of N2b by 3 cm threshold.ConclusionA new N‐classification improved staging performance compared to TNM‐8‐N, by stratifying by ENE extent, eliminating the old N2c category and the 6 cm threshold, and by stratifying multiple nodes by size.
Meta‐analysis of sex and racial subgroup participation rates and differential treatment effects for trials in solid tumor malignancies leading to US Food and Drug Administration registration between 2010 and 2021
Authors: Wilson Brooke E. , Nadler Michelle B. , Desnoyers Alexandra , Booth Christopher M. , Amir Eitan 
Published: September 2023

AbstractBackgroundThe lack of sociodemographic diversity in clinical trials limits the generalizability of results. The authors examined participation rates and effect modification by sex and race in oncology trials.MethodsThe authors extracted outcome data stratified by sex and race for registration trials supporting US Food and Drug Administration (FDA) approval (2010–2021). Effect modification by race and sex was examined using quantitative and qualitative methods. A random‐effects meta‐analysis and pairwise comparison of progression‐free survival (PFS) and overall survival (OS) outcomes was conducted by sex and race.ResultsNinety‐five trials with 123 end points and 54,365 patients provided information on sex. Trial patients were more often male (n = 35,482; 65% vs. 56% male patients in US Surveillance, Epidemiology, and End Results [SEER] data), although the proportion of male patients was similar after adjusting by tumor type (60% in FDA data vs. 58% in SEER data). There was no difference in pooled outcomes among male versus female patients (PFS: hazard ratio, 0.99; 95% confidence interval, 0.92–1.07; p = .89; OS: hazard ratio, 0.99; 95% confidence interval, 0.93–1.07; p = .90). In total, 111 trials including 74,217 patients provided information on race, and 68% of patients identified as White, compared with 72.3% in US SEER incidence data. Black patients were under‐represented compared with US SEER incidence data, although ethnicity was poorly reported throughout the data set. In the authors' network meta‐analysis by race, there were no statistically significant differences in PFS or OS outcomes.ConclusionsNo significant differences in PFS or OS outcomes were identified when the analyses were stratified by sex or race. Certain racial minorities remain under‐represented, and clearer reporting of race and ethnicity is needed. Representation of female patients in FDA trials is similar to that in SEER data after adjusting for tumor type.
Tumor‐infiltrating lymphocyte transfusion in a patient with treatment refractory triple negative breast cancer
Authors: Jacover Arielle , Zarbiv Yonaton , Tal Keidar Haran , Klein Shira , Breuer Shani , Durst Ronen , Avni Batia , Grisariu Sigal , Stepensky Polina , Lotem Michal , Maimon Ofra , Yablonski‐Peretz Tamar 
Published: September 2023

AbstractBackgroundTriple negative breast cancer (TNBC) is an aggressive form of breast cancer that is treated with chemotherapy. Recently, programmed death 1 (PD1) inhibition, as well as antibody‐drug conjugates, have been added to the available treatment regimen, yet metastatic disease is fatal. Adoptive cell therapy (ACT) using tumor infiltrating lymphocytes (TILs) has been well described in melanoma, but less data is available on other solid malignancies.CaseHerein, we present a case of a 31‐year‐old patient diagnosed with Breast Cancer gene 1 (BRCA1) positive, TNBC. The patient's disease rapidly progressed while under standard treatment protocols. As a result, additional genetic testing of the tumor was carried out and revealed loss of BRCA1 heterozygosity, a double Tumor Protein 53 (TP53) mutation, and MYC amplification. Due to resistance to conventional therapy, an experimental approach was attempted using tumor‐infiltrating lymphocytes in November 2021 at Hadassah University Medical Center. While receiving this treatment, the patient exhibited a reported subjective clinical improvement including a month spent out of the hospital. However, the final result, presumably due to Interleukin 2 (IL‐2) toxicity, was the patient's passing.ConclusionThis case is unique and peculiar regarding the treatment modality chosen, due to the extremely refractory disease the patient suffered from. After standard therapies rapidly failed, adoptive cell therapy was attempted with the infusion of TILs. This treatment has been shown effective in melanoma, however, there is an extreme paucity of data on other solid tumors, including TNBC. Although the patient ultimately demised presumably due to treatment side effects, brief clinical benefit was apparent. Further studies are warranted.
Quantum secret sharing protocol through noisy channel with application in visual cryptography
Authors: Rathi Deepa , Kumar Sanjeev , Musanna Farhan , Singh Deepak 
Published: September 2023

SummaryThis study presents a quantum secret sharing (QSS) protocol designed using Grover's search algorithm in a noisy environment. The proposed protocol utilizes Grover's three‐particle quantum state. The proposed scheme is divided into secret information sharing and eavesdropping checking. The dealer prepares an encoded state by encoding the classical information as a marked state and shares the states' qubits between three participants. Using the amplitude‐damping noise and the phase‐damping noise as conventional noisy channels, it can be demonstrated that secret information can be conveyed between participants with some information lost. The security analysis shows the scheme is stringent against malicious participants or eavesdroppers. The simulation analysis is done on the cloud platform IBM‐QE thereby showing the practical feasibility of the scheme. Finally, an application of the proposed scheme is demonstrated in visual cryptography using the GNEQR representation of images.
In Situ DRIFTS Analysis during Hydrogenation of 1‐Pentyne and Olefin Purification with Ag Nanoparticles
Authors: Cordoba Misael , Garcia Lina , Badano Juan , Betti Carolina , Coloma‐Pascual Fernando , Quiroga Mónica , Lederhos Cecilia 
Published: September 2023

AbstractThe catalytic performance of nanoparticles (NPs) of Ag anchored on different supports was evaluated during the selective hydrogenation of 1‐pentyne and the purification of a mixture of 1‐pentene/1‐pentyne (70/30 vol %). The catalysts were identified: Ag/Al (Ag supported on ɣ‐Al2O3), Ag/Al−Mg (Ag supported on ɣ‐Al2O3 modified with Mg), Ag/Ca (Ag supported on CaCO3) and Ag/RX3 (Ag supported on activated carbon‐type: RX3). In addition, in situ DRIFTS analysis of 1‐pentyne adsorption on each support, catalyst, and 1‐pentyne hydrogenation were investigated. The results showed that the synthesized catalysts were active and very selective (≥85 %) for obtaining the desired product (1‐pentene). Different adsorbed species (−C≡C− and −C=C−) were observed on the supports and catalysts surface using in situ DRIFT analysis, which can be correlated to the activity and high selectivity reached. The role of the supports and electronic properties over Ag improve the H2 dissociative chemisorption during the hydrogenation reactions; promoting the selectivity and the high catalytic performance. Ag/Al and Ag/Al−Mg were the most active catalysts. This was due to the synergism between the active Ag/Ag+ species and the supports (electronic effects). The results show that Ag/Al and Ag/Al−Mg catalysts have favorable properties and are promising for the alkyne hydrogenation and olefin purification reactions.
Return on investment of the International Potato Center–led seed system interventions in Malawi
Authors: Feukeng Florette Tsuanyo , Otieno David Jakinda , Rajendran Srinivasulu , Demo Paul , Parker Monica 
Published: September 2023

AbstractWith declining funding in agricultural research and development coupled with tremendous pressure from different stakeholders, it is now essential for research institutes to demonstrate the return on investments (RoIs) in projects that they implement. In response to the above need, this study evaluated the RoI of seed sector interventions implemented by the International Potato Center in Malawi with the financial support of 3.425 million euros from Irish Aid, from 2007 to 2017. The project interventions evaluated in this study were seed selection, seed multiplication technologies, and potato (Solanum tuberosum) varieties. The study used the economic surplus model to measure monetary benefits realized by the project, while a cost–benefit analysis was employed to estimate the RoI. Results revealed positive returns on donors’ investments over the project's lifespan. The net project worth was estimated at $66.6 million when net benefits are discounted by 6% and $71.8 million when net benefits are discounted by 12% over 23 years project benefit lifespan (2007–2030). The basic RoI found a $6 to $8 return considering a 6% and 12% discount rate, respectively, for every dollar invested. Despite these positive results, further investments are needed in the seed production system for the early seed generations. Effective regulatory interventions on seed quality assurance and continuous awareness creation efforts are required to encourage farmers to use the best seed production methods.
Reactivity Studies of Cyclic Alkyl(Amino) Carbene (cAAC)‐Supported Phosphinidenide with AuCl
Authors: Nag Ekta , Francis Maria , Roy Sudipta 
Published: September 2023

AbstractA neutral octa‐nuclear AuI‐cluster (2) with molecular formula [(Cy‐cAACPAu)4(AuCl)4] [Cy‐cAAC=:C(N‐2,6‐iPr2C6H3)(C6H10)(CMe2)(CH2)] has been isolated, and structurally characterized by single‐crystal X‐ray diffraction. 2 has been further studied by NMR and ESI‐MS. The μ3‐P bridged AuI‐cluster 2 possesses a C2 axis that passes through the two Au atoms positioned oppositely of the central AuI4P4 core, and two carbene‐P chromophores. The emission spectrum of 2 exhibited a broad band with λem at 720 nm, and an average lifetime of 3.36 ns when excited at 388 nm in solid state at room temperature. Cyclic voltammetry (CV) studies revealed that 2 can undergo reversible one‐electron oxidation at 0.76 V. The electronic transition in 2 has been further studied by computational methods.
Using a Bayesian Network Model to Predict Risk of Pesticides on Aquatic Community Endpoints in a Rice Field—A Southern European Case Study
Authors: Mentzel Sophie , Martínez-Megías Claudia , Grung Merete , Rico Andreu , Tollefsen Knut Erik , Van den Brink Paul J. , Moe S. Jannicke 
Published: September 2023

Abstract
Bayesian network (BN) models are increasingly used as tools to support probabilistic environmental risk assessments (ERAs), because they can better account for uncertainty compared with the simpler approaches commonly used in traditional ERA. We used BNs as metamodels to link various sources of information in a probabilistic framework, to predict the risk of pesticides to aquatic communities under given scenarios. The research focused on rice fields surrounding the Albufera Natural Park (Valencia, Spain), and considered three selected pesticides: acetamiprid (an insecticide), 2-methyl-4-chlorophenoxyacetic acid (MCPA; a herbicide), and azoxystrobin (a fungicide). The developed BN linked the inputs and outputs of two pesticide models: a process-based exposure model (Rice Water Quality [RICEWQ]), and a probabilistic effects model (Predicts the Ecological Risk of Pesticides [PERPEST]) using case-based reasoning with data from microcosm and mesocosm experiments. The model characterized risk at three levels in a hierarchy: biological endpoints (e.g., molluscs, zooplankton, insects, etc.), endpoint groups (plants, invertebrates, vertebrates, and community processes), and community. The pesticide risk to a biological endpoint was characterized as the probability of an effect for a given pesticide concentration interval. The risk to an endpoint group was calculated as the joint probability of effect on any of the endpoints in the group. Likewise, community-level risk was calculated as the joint probability of any of the endpoint groups being affected. This approach enabled comparison of risk to endpoint groups across different pesticide types. For example, in a scenario for the year 2050, the predicted risk of the insecticide to the community (40% probability of effect) was dominated by the risk to invertebrates (36% risk). In contrast, herbicide-related risk to the community (63%) resulted from risk to both plants (35%) and invertebrates (38%); the latter might represent (in the present study) indirect effects of toxicity through the food chain. This novel approach combines the quantification of spatial variability of exposure with probabilistic risk prediction for different components of aquatic ecosystems. Environ Toxicol Chem 2024;43:182–196. © 2023 The Authors. Environmental Toxicology and Chemistry published by Wiley Periodicals LLC on behalf of SETAC.