bluuebunny/crossref_metadata_2025_split huggingface dataset:
Expanded profiling of Remdesivir as a broad-spectrum antiviral and low potential for interaction with other medications in vitro
Authors: Radoshitzky Sheli R. , Iversen Patrick , Lu Xianghan , Zou Jing , Kaptein Suzanne J. F. , Stuthman Kelly S. , Van Tongeren Sean A. , Steffens Jesse , Gong Ruoyu , Truong Hoa , Sapre Annapurna A. , Yang Huiling , Xie Xiaodong , Chia Jia Jun , Song Zhijuan J. , Leventhal Stacey M. , Chan Josolyn , Shornikov Alex , Zhang Xin , Cowfer David , Yu Helen , Warren Travis , Cihlar Tomas , Porter Danielle P. , Neyts Johan , Shi Pei-Yong , Wells Jay , Bilello John P. , Feng Joy Y. 
Published: February 2023

AbstractRemdesivir (GS-5734; VEKLURY) is a single diastereomer monophosphoramidate prodrug of an adenosine analog (GS-441524). Remdesivir is taken up by target cells and metabolized in multiple steps to form the active nucleoside triphosphate (GS-443902), which acts as a potent inhibitor of viral RNA-dependent RNA polymerases. Remdesivir and GS-441524 have antiviral activity against multiple RNA viruses. Here, we expand the evaluation of remdesivir’s antiviral activity to members of the families Flaviviridae, Picornaviridae, Filoviridae, Orthomyxoviridae, and Hepadnaviridae. Using cell-based assays, we show that remdesivir can inhibit infection of flaviviruses (such as dengue 1–4, West Nile, yellow fever, Zika viruses), picornaviruses (such as enterovirus and rhinovirus), and filoviruses (such as various Ebola, Marburg, and Sudan virus isolates, including novel geographic isolates), but is ineffective or is significantly less effective against orthomyxoviruses (influenza A and B viruses), or hepadnaviruses B, D, and E. In addition, remdesivir shows no antagonistic effect when combined with favipiravir, another broadly acting antiviral nucleoside analog, and has minimal interaction with a panel of concomitant medications. Our data further support remdesivir as a broad-spectrum antiviral agent that has the potential to address multiple unmet medical needs, including those related to antiviral pandemic preparedness.
Wound swab quality grading is dependent on Gram smear screening approach
Authors: Clark Shawn T. , Forbes Jessica D. , Matukas Larissa M. 
Published: February 2023

AbstractSuperficial skin swab collections are inherently low-quality and may be of little clinical value due to their poor sensitivity and specificity. Clinical microbiology laboratories can use Gram smears to screen and differentiate higher and lower quality specimens to direct the extent of potential pathogen work up, including antimicrobial susceptibility testing (AST). We compared the impact of two different smear grading approaches to our current reporting practices for superficial wound swab cultures. Two variations of the Q score methodology (low power under 10X (QS10) and high power under 100X (QS100) were compared to our existing oil immersion method (OM100) (100X). We further evaluated the QS100 method by scoring superficial swab smears previously screened by OM100 from cultures submitted between November 2018 and December 2019. No significant difference in the number of low-quality specimens (N = 50) was identified by QS10 or QS100 grading (N = 9; 18%; N = 8; 16% respectively). Among 968 additional QS100 screened smears, 67 (6.9%) low quality swabs were identified and 7.4% fewer organisms (76/1020 organisms) would require reporting with AST. Implementing the Q score for superficial wound swab cultures would provide minimal improvements in their clinical relevance, laboratory quality and efficiency in our laboratory due to the low number of poor-quality swabs received.
Induction of primordial germ cell-like cells from common marmoset embryonic stem cells by inhibition of WNT and retinoic acid signaling
Authors: Shono Mayumi , Kishimoto Keiko , Hikabe Orie , Hayashi Masafumi , Semi Katsunori , Takashima Yasuhiro , Sasaki Erika , Kato Kiyoko , Hayashi Katsuhiko 
Published: February 2023

AbstractReconstitution of the germ cell lineage using pluripotent stem cells provides a unique platform to deepen our understanding of the mechanisms underlying germ cell development and to produce functional gametes for reproduction. This study aimed to establish a culture system that induces a robust number of primordial germ cell-like cells (PGCLCs) from common marmoset (Callithrix jacchus) embryonic stem cells. The robust induction was achieved by not only activation of the conserved PGC-inducing signals, WNT and BMP4, but also temporal inhibitions of WNT and retinoic acid signals, which prevent mesodermal and neural differentiation, respectively, during PGCLC differentiation. Many of the gene expression and differentiation properties of common marmoset PGCLCs were similar to those of human PGCLCs, making this culture system a reliable and useful primate model. Finally, we identified PDPN and KIT as surface marker proteins by which PGCLCs can be isolated from embryonic stem cells without genetic manipulation. This study will expand the opportunities for research on germ cell development and production of functional gametes to the common marmoset.
Integrative taxonomy and species distribution models of the genus Diamesus Hope, 1840 (Coleoptera: Staphylinidae: Silphinae)
Authors: Růžička Jan , Jakubec Pavel , Mahlerová Karolina , Šípková Hana , Nishikawa Masaaki 
Published: February 2023

AbstractIntegrative taxonomy ofDiamesusHope, 1840 (Coleoptera: Silphinae) is presented. Adults ofD. bimaculatusPortevin, 1914 (endemic to Taiwan) andD. osculans(Vigors, 1825) (widely distributed from northern India to Australia) are redescribed, keyed and figured, including characters of the male and female genitalia of both species. Variation in elytral maculation inD. osculansis discussed and illustrated. The absence of diagnostic differences ofD.osculansvar.reductusPic, 1917 fromD. osculansis discussed, and the former name is confirmed as a junior subjective synonym ofD. osculans. Types of all three names available were studied; a lectotype and paralectotypes are designated for the nameD. osculansvar.bimaculatusPortevin, 1914. Molecular phylogenetic analysis confirms the genusDiamesusis sister group to the genusNecrodesLeach, 1815, andD. osculansandD. bimaculatusare two, well supported clades. Detailed data on the distribution ofD. bimaculatusandD. osculansare presented and mapped. Species distribution models for both species were created and interpreted.Diamesus osculansis reported for the first time from India: Uttarakhand, China: Anhui, Hainan, Hunan, Jiangxi, Shaanxi and Zhejiang Provinces, and Australia: Victoria; it is also recently confirmed from Taiwan, being sympatric in distribution there withD. bimaculatus. Available data on the ecology and seasonality of both species ofDiamesusare also discussed.
Facile preparation of flame-retardant cellulose composite with biodegradable and water resistant properties for electronic device applications
Authors: Chandrasekaran Saravanan , Cruz-Izquierdo Alvaro , Castaing Remi , Kandola Baljinder , Scott Janet L. 
Published: February 2023

AbstractThe aim of the present study is to produce flexible, flame-retardant, water-resistant and biodegradable composite materials. The ultimate goal of this research is to develop simple processes for the production of bio-based materials capable of replacing non-degradable substrates in printed circuit board. Cellulose was chosen as a renewable resource, and dissolved in 1-ethyl-3-methylimidazolium acetate ionic liquid to prepare a cellulosic continuous film. Since flame retardancy is an important criterion for electronic device applications and cellulose is naturally flammable, we incorporated ammonium polyphosphate (APP) as a flame-retardant filler to increase the flame retardancy of the produced materials. The developed material achieved a UL-94 HB rating in the flammability test, while the cellulose sample without APP failed the test. Two hydrophobic agents, ethyl 2-cyanoacrylate and trichloro(octadecyl)silane were applied by a simple dip-coating technique to impart hydrophobicity to the cellulose-APP composites. Dynamic mechanical analysis indicated that the mechanical properties of the cellulosic materials were not significantly affected by the addition of APP or the hydrophobic agents. Moreover, the biodegradability of the cellulosic materials containing APP increased owing to the presence of the cellulase enzyme. The hydrophobic coating slightly decreased the biodegradability of cellulose-APP, but it was still higher than that of pure cellulose film.
Hippocampal volumetry to determine the resection side in patients with intractable non-lesional bilateral temporal lobe epilepsy
Authors: Seyama Go , Iida Koji , Kagawa Kota , Katagiri Masaya , Okamura Akitake , Morioka Hiromi , Horie Nobutaka 
Published: February 2023

AbstractBilateral Temporal lobe epilepsy (BTLE) cases may result in poor surgical outcomes due to the difficulty in determining/localizing the epileptogenic zone. In this study, we investigated whether hippocampal volume (HV) would be useful for the determination of the best resection side in BTLE. Eighteen cases of BTLE determined by a scalp video electroencephalogram (SVEEG) underwent resection via intracranial electroencephalography (IVEEG). Patients with lesions or semiologically determined focus lateralization were excluded. In addition to SVEEG, an epilepsy protocol magnetic resonance imaging (MRI) including hippocampus fluid-attenuated inversion recovery (FLAIR) and HV, 18F-fluorodeoxyglucose positron emission tomography (FDG-PET), single-photon emission computed tomography with 123I-iomazenil (IMZ-SPECT), and magnetoencephalography (MEG) were performed for the preoperative evaluation of the lateralization. The resection side was determined based on the IVEEG results, and the seizure outcome at two years postoperatively was classified as either a well-controlled seizure outcome (Engel class I), or residual (classes II–V). We used a Fisher's exact test to compare the concordance between the determination of the epileptic focus by each modality and the resected side where patients achieved a well-controlled seizure outcome. Seizures were well controlled in 9/18 patients after surgery. Eight out of 11 patients (72.7%), in whom the HV results (strongly atrophic side) and the resection side were matched, had well-controlled seizure outcomes (P = 0.0498). The concordance of other presurgical evaluations with the resection side was not significantly related to a well-controlled seizure outcome. HV may be a useful method to determine the optimal resection side of the epileptic focus/foci in cases of suspected BTLE.
Study of the anticorrosive behavior of samarium as a corrosion inhibitor in multilayer systems for aluminum alloy
Authors: Cabello Mendez José Antonio , Arguelles Rojas Ailed , Pérez Bueno José de Jesús , Meas Vong Yunny 
Published: February 2023

AbstractThis study shows a multilayer system based on samarium compounds as a corrosion inhibitor and a continuous SiO2 layer by atmospheric pressure plasma jet (APPJ) as a protective barrier for aluminim alloy AA3003. One of the main advantages of this new coating is that it does not require vacuum chambers, which makes it easy to incorporate into production lines for automotive and aeronautical components, etc. The deposit of samarium corrosion inhibitor was carried out by two methods for comparison, the immersion method and a novel method to deposit corrosion inhibitor by APPJ. The multilayer system generated was homogeneous, continuous, adherent, and dense. The electrochemical behavior shows that the samarium compound was completely oxidized on coatings by the immersion method and favors corrosion. The APPJ deposition method shows a protective behavior against corrosion by both samarium compounds and silica depositions. XPS analyses show that the amount of Sm(OH)3 increases by the APPJ method compared with the immersion method since the spectrum of O1s is mainly controlled by OH. It was determined that the best processing times for the electrochemical study of the multilayer system were 40 min for the immersion method and 30 s for the APPJ method for the layer of corrosion inhibitor. In the case of the SiO2 barrier layer by APPJ, the best time was 60 s of exposure to the plasma jet and this coating could reduce the corrosion of AA3003 by 31.42%.
A facile novel synthesis of AgCuO2 delafossite nanoparticles and evaluation of their antimicrobial activity
Authors: Ateia Ebtesam E. , Arman M. M. , Mohamed Amira T. 
Published: February 2023

AbstractBi-functional nano-oxides are of growing interest to address environmental issues. In the present study, the structural and magnetic data are presented together with the antimicrobial activities (AMA). For the first time, silver delafossite oxide (AgCuO2) is successfully fabricated using a simple, low-cost technique to target antibiotic photodegradation and inactivation of model waterborne pathogens. It is prepared with an equimolar initial Ag+:Cu+ concentration ratio. The structure, morphology, and magnetic properties are studied by different characterization techniques. The size and shape of AgCuO2 NPs, in addition to their structural polytypes of 2H (hexagonal) or 3R (rhombohedral), are dependent on the preparation conditions. The existence of Cu, Ag, and O in the synthesized delafossite AgCuO2 NPs with no evidence of any impurity is ratified by the XPS spectrum. AFM measurements are taken to characterize the surface morphologies of AgCuO2. The distributed spiks are evaluated by roughness kurtosis (Rku). The roughness kurtosis has a value of 2.65 (< 3), indicating that the prepared sample is classified as bumpy. The prepared sample has 13.0, 10.0, 14.0, and 14.0 mm Inhibition Zone Diameter (IZD) antimicrobial activity against gram-positive Bacillus subtilis (B. subtilis), Bacillus cereus (B. cereus), Enterococcus faecalis (E. faecalis), and Staphylococcus aureus (S. aureus), respectively. The IZD for gram-negative Escherichia coli (E. coli), Neisseria Gonorrhoeae (N. Gonorrhoeae), Pseudomonas aeruginosa (P. aeruginosa), and Salmonella typhimrium (S. typhimrium) were found to be 12.0, 13.0, 14.0, and 13.0 mm, respectively. Therefore, the AgCuO2 NPs reveal excellent antimicrobial efficiency, and they can be effortlessly separated using a tiny magnet or a simple magnetic separator. The adequate cytotoxicity and magnetic characteristics of the antimicrobial sample suggest a promising future for it in biomedical applications.
Identifying important nodes in complex networks based on extended degree and E-shell hierarchy decomposition
Authors: Liu Jun , Zheng Jiming 
Published: February 2023

AbstractThe identification of important nodes is a hot topic in complex networks. Many methods have been proposed in different fields for solving this problem. Most previous work emphasized the role of a single feature and, as a result, rarely made full use of multiple items. This paper proposes a new method that utilizes multiple characteristics of nodes for the evaluation of their importance. First, an extended degree is defined to improve the classical degree. And E-shell hierarchy decomposition is put forward for determining nodes’ position through the network’s hierarchical structure. Then, based on the combination of these two components, a hybrid characteristic centrality and its extended version are proposed for evaluating the importance of nodes. Extensive experiments are conducted in six real networks, and the susceptible–infected–recovered model and monotonicity criterion are introduced to test the performance of the new approach. The comparison results demonstrate that the proposed new approach exposes more competitive advantages in both accuracy and resolution compared to the other five approaches.
Influence of surface carbon on the performance of cesiated p-GaN photocathodes with high quantum efficiency
Authors: Schaber Jana , Xiang Rong , Teichert Jochen , Arnold André , Murcek Petr , Zwartek Paul , Ryzhov Anton , Ma Shuai , Gatzmaga Stefan , Michel Peter 
Published: February 2023

AbstractThis study shows residual surface carbon’s influence on photocathodes’ quantum efficiency based on p-GaN grown on sapphire by metal organic chemical vapor deposition. An X-ray photoelectron spectrometer (XPS) built in an ultrahigh vacuum system allowed the in-situ monitoring of the photocathode surface beginning immediately after their cleaning and throughout the activation and degradation processes. An atomically clean surface is necessary to achieve a negative electron affinity, which is the main prerequisite for high quantum efficiency. The p-GaN samples were cleaned with ethanol and underwent a sub-sequential thermal vacuum cleaning. Although carbon and oxygen contaminations are expected to be undesired impurities from the metal organic chemical vapor deposition, which remained on the surface, p-GaN could still form a negative electron affinity surface when exclusively activated with cesium. After the activation with cesium, a shift to a higher binding energy of the photoemission peaks was observed, and a new species, a so-called cesium carbide, was formed, growing over time. The XPS data elucidated the critical role of these cesium carbide species in photocathode degradation. The X-ray damage to the p-GaN:Cs photocathodes, especially the influence on the cesium, was additionally discussed.
Predictive models in emergency medicine and their missing data strategies: a systematic review
Authors: Arnaud Emilien , Elbattah Mahmoud , Ammirati Christine , Dequen Gilles , Ghazali Daniel Aiham 
Published: February 2023

AbstractIn the field of emergency medicine (EM), the use of decision support tools based on artificial intelligence has increased markedly in recent years. In some cases, data are omitted deliberately and thus constitute “data not purposely collected” (DNPC). This accepted information bias can be managed in various ways: dropping patients with missing data, imputing with the mean, or using automatic techniques (e.g., machine learning) to handle or impute the data. Here, we systematically reviewed the methods used to handle missing data in EM research. A systematic review was performed after searching PubMed with the query “(emergency medicine OR emergency service) AND (artificial intelligence OR machine learning)”. Seventy-two studies were included in the review. The trained models variously predicted diagnosis in 25 (35%) publications, mortality in 21 (29%) publications, and probability of admission in 21 (29%) publications. Eight publications (11%) predicted two outcomes. Only 15 (21%) publications described their missing data. DNPC constitute the “missing data” in EM machine learning studies. Although DNPC have been described more rigorously since 2020, the descriptions in the literature are not exhaustive, systematic or homogeneous. Imputation appears to be the best strategy but requires more time and computational resources. To increase the quality and the comparability of studies, we recommend inclusion of the TRIPOD checklist in each new publication, summarizing the machine learning process in an explicit methodological diagram, and always publishing the area under the receiver operating characteristics curve—even when it is not the primary outcome.
The unequal burden of human-wildlife conflict
Authors: Braczkowski Alexander R. , O’Bryan Christopher J. , Lessmann Christian , Rondinini Carlo , Crysell Anna P. , Gilbert Sophie , Stringer Martin , Gibson Luke , Biggs Duan 
Published: February 2023

AbstractHuman-wildlife conflict is one of the most pressing sustainable development challenges globally. This is particularly the case where ecologically and economically important wildlife impact the livelihoods of humans. Large carnivores are one such group and their co-occurrence with low-income rural communities often results in real or perceived livestock losses that place increased costs on already impoverished households. Here we show the disparities associated with the vulnerability to conflict arising from large carnivores on cattle (Bos taurus) globally. Across the distribution of 18 large carnivores, we find that the economic vulnerability to predation losses (as measured by impacts to annual per capita income) is between two and eight times higher for households in transitioning and developing economies when compared to developed ones. This potential burden is exacerbated further in developing economies because cattle keepers in these areas produce on average 31% less cattle meat per animal than in developed economies. In the lowest-income areas, our estimates suggest that the loss of a single cow or bull equates to nearly a year and a half of lost calories consumed by a child. Finally, our results show that 82% of carnivore range falls outside protected areas, and five threatened carnivores have over one third of their range located in the most economically sensitive conflict areas. This unequal burden of human-carnivore conflict sheds light on the importance of grappling with multiple and conflicting sustainable development goals: protecting life on land and eliminating poverty and hunger.
RhoGDIα regulates spermatogenesis through Rac1/cofilin/F-actin signaling
Authors: Zhu Haixia , Wen Zongzhuang , Zhang Aizhen , Liu Dongyue , Wang Hongxiang , Cheng Yin , Yang Xing , Xiao Yu , Li Jianyuan , Sun Daqing , Wu Bin , Gao Jiangang 
Published: February 2023

AbstractSpermatogenesis is an extremely complex process, and any obstruction can cause male infertility. RhoGDIα has been identified as a risk of male sterility. In this study, we generate RhoGDIα knockout mice, and find that the males have severely low fertility. The testes from RhoGDIα−/− mice are smaller than that in WT mice. The numbers of spermatogonia and spermatocytes are decreased in RhoGDIα−/− testis. Spermatogenesis is compromised, and spermatocyte meiosis is arrested at zygotene stage in RhoGDIα−/− mice. Acrosome dysplasia is also observed in sperms of the mutant mice. At the molecular level, RhoGDIα deficiency activate the LIMK/cofilin signaling pathway, inhibiting F-actin depolymerization, impairing testis and inducing low fertility in mouse. In addition, the treatment of RhoGDIα−/− mice with Rac1 inhibitor NSC23766 alleviate testis injury and improve sperm quality by inhibiting the LIMK/cofilin/F-actin pathway during spermatogenesis. Together, these findings reveal a previously unrecognized RhoGDIα/Rac1/F-actin-dependent mechanism involved in spermatogenesis and male fertility.
RAGA prevents tumor immune evasion of LUAD by promoting CD47 lysosome degradation
Authors: Zhang Lian , Yu Jing , Zheng Mingyue , Zhen Hui , Xie Qingqiang , Zhang Chundong , Zhou Zhongjun , Jin Guoxiang 
Published: February 2023

AbstractCD47 is a macrophage-specific immune checkpoint protein acting by inhibiting phagocytosis. However, the underlying mechanism maintaining CD47 protein stability in cancer is not clear. Here we show that CD47 undergoes degradation via endocytosis/lysosome pathway. The lysosome protein RAGA interacts with and promotes CD47 lysosome localization and degradation. Disruption of RAGA blocks CD47 degradation, leading to CD47 accumulation, high plasma membrane/intracellular CD47 expression ratio and reduced phagocytic clearance of cancer cells. RAGA deficiency promotes tumor growth due to the accumulation of CD47, which sensitizes the tumor to CD47 blockade. Clinical analysis shows that RAGA and CD47 proteins are negatively correlated in lung adenocarcinoma patient samples. High RAGA protein level is related to longer patient survival. In addition, RAGAhighCD47low patients show the longest overall survival. Our study thereby not only reveals a mechanism by which RAGA regulates CD47 lysosome degradation, but also suggests RAGA is a potential diagnostic biomarker of lung adenocarcinoma.