bluuebunny/crossref_metadata_2025_split huggingface dataset:
Restraint stress promotes lung cancer xenograft growth via the IL2-Ras-Erk pathway
Authors: Xu Ke , Li Meng , Zheng Cheng , Ji Miao , Wu Wenjing , Huang Xin , Shen Fangfang , Teng Yiqun , Zhang Jin 
Published: February 2023

Abstract
Objectives To assess the impact of restraint stress on lung cancer xenograft development, and explore the Molecular mechanism.
Methods We established a restraint stress model by placing nude mice in a ventilated 50 mL centrifuge tube and inoculating them with subcutaneous xenografts of the A549 lung cancer cell line on day 21. In order to verify the effects of IL2 on tumor growth, we treated A549 cells with IL2 (1 or 5 ng/mL) in vitro.
Results Compared with the non-stressed mice, the stressed mice exhibited lower body weight gain and larger tumors after 42 days of restraint stress treatment. The stressed mice also exhibited a higher level of proinflammatory cytokine IL2 in serum, tumor tissue, spleen and lymph nodes. The tumors from the stressed mice exhibited higher activity of the ras-extracellular signal-regulated kinase (Ras-Erk) signaling pathway. Compared with the control group (0 ng/mL), the cells treated with IL2 exhibited a higher rate of proliferation and migration, along with increased activity of the Ras-Erk pathway. Knockdown of the IL2 receptor by siRNA alleviated the increase of proliferation, migration and RAS-Erk pathway activity stimulated by IL2.
Conclusion Based on these findings, we conclude that restraint stress increased IL2 levels to promote tumor growth by activating the Ras-Erk pathway.
Possible secondary craniosynostosis caused by flat head syndrome: Report of two cases
Authors: Tomohito NAGAI , Toshiaki HAYASHI , Tomomi KIMIWADA , Junji TAKEYAMA , INUKAI Madoka , Teiji TOMINAGA 
Published: February 2023

Abstract
Standard treatment for patients with craniosynostosis is cranial remodeling surgery. If diagnosed early, minimally invasive surgery, such as suturectomy, is a good alternative, although it is sometimes difficult to diagnose craniosynostosis early because premature suture closure is not always radiologically obvious. In this study, we present two cases of five-month old infants who were conservatively treated for positional brachycephaly without any obvious premature suture closure on CT, who later developed progressive tower-like skull deformity despite conservative treatment for positional skull deformity. Both their fathers showed similar skull deformities. They underwent suturectomy at the site of the lambda, followed by molding helmet therapy based on a diagnosis of craniosynostosis. Histopathological specimens obtained from the resected sutures showed irregularly narrowed suture structure and ossification and fibrous tissue proliferation within it, supporting the diagnosis. The infants’ postoperative course was uneventful. The cephalic index of both the cases improved postoperatively. Conservative therapy-resistant progressive occipital skull deformity can sometimes lead to secondary craniosynostosis, even if CT does not show premature suture closure.
Bone mineral density and bone microarchitecture assessment using trabecular bone score in Acromegaly
Authors: Boro Hiya , Nagendra Lakshmi , Mondal Sunetra , Mannar Velmurugan , Alam Sarah , Kubihal Suraj , Dogra Vinay , Khatiwada Saurav , Malhotra Rakhi 
Published: February 2023

Abstract
Purpose
We aimed to analyze the bone mineral density (BMD) and bone microarchitecture using the trabecular bone score (TBS) in acromegaly and compare it with healthy controls.
Methods
The current study included 50 patients with active acromegaly and 50 age and sex-matched controls. Biochemical parameters were analyzed using standardized techniques. BMD was assessed using dual-energy X-ray absorptiometry (DXA). (Discovery A 84023, Hologic Inc, MA, USA). TBS of the lumbar spine was measured using TBS iNsight software (version 3.0.2.0, medimaps, Merignac, France) installed on the same DXA machine.
Results
Acromegaly patients had a significantly greater lumbar spine [(1.026 ± 0.180) g/cm2 vs (0.951 ± 0.108) g/cm2, p=0.014], femoral neck [(0.879 ± 0.136) g/cm2 vs (0.780 ± 0.110) g/cm2, p<0.001], and total hip BMD [(1.013 ± 0.128) g/cm2 vs (0.917 ± 0.118) g/cm2, p<0.001] than healthy controls while no significant difference was observed in the distal radius BMD [(0.700 ± 0.102) g/cm2 vs (0.698 ± 0.087) g/cm2, p=0.890] and TBS [(1.348 ± 0.086) vs (1.345 ± 0.091), p=0.890].
No significant difference was observed in the TBS between eugonadal and hypogonadal acromegaly cases, be they male or female. On multiple linear regression analysis, ‘age’ and ‘lumbar spine BMD’ were found to predict TBS in acromegaly.
Conclusion
Our study showed no difference in TBS between acromegaly and controls, irrespective of gender and gonadal status. Further studies (longitudinal) with the involvement of a large sample size are required to analyze the utility of TBS in the prediction of skeletal health and fracture risk in acromegaly.
Association between urinary methylparaben level and bone mineral density in children and adolescents aged 8-19 years
Authors: He Huan , Li Min , Liu Bailing , Zhang Zhan 
Published: February 2023

Abstract
Background
Previous epidemiological studies have explored the relationships between methylparaben (Mep) and bone mineral density (BMD) in adults. However, the correlation between methylparaben and BMD in children and adolescents is unclear.
Objective
This study examined the correlation between Mep and BMD in children and adolescents aged 8–19 years.
Methods
Data on urinary Mep levels and BMD (containing total BMD, trunk bone BMD, pelvis BMD, lumbar spine BMD and thoracic spine BMD) of 1,830 children and adolescents aged 8–19 years were obtained from the 2011–2016 National Health and Nutrition Examination Survey (NHANES) database. Multivariable linear regressions and smooth curve fittings were adopted to evaluate the correlation between Mep and BMD.
Results
After adjusting potential confounders, we found positive relationship between Mep and BMD. Furthermore, the positive trends were significant in females aged 8–19 years, but not significant in males aged 8–19 years (P for trend > 0.05).
Conclusion
This study has been the first study that found evidence to demonstrate that exposure to Mep may be related to BMD in children and adolescents aged 8–19 years. More population and experimental studies should be performed to verify the above findings and analyze the mechanism.
HDAC3 deficiency in macrophage attenuates acute lung injury by inhibiting cGAS-mediated pyroptosis in a miR-4767-dependent manner
Authors: Li Ning , Liu Bohao , He Ruyuan , Li Guorui , Xiong Rui , Fu Tinglv , Li Donghang , Wang Bo , Geng Qing 
Published: February 2023

Abstract
Activated inflammation and pyroptosis in macrophage are closely
associated with acute lung injury (ALI). Histone deacetylase 3
(HDAC3) serves as an important enzyme that could repress gene
expression by mediating chromatin remodeling. Here, we aim to
explore the role and potential molecular basis of HDAC3 in
lipopolysaccharide (LPS)-induced ALI. We found that HDAC3 was
highly expressed in lung tissues of LPS-treated mice and in
LPS-induced macrophages. Lung tissues from macrophage
HDAC3-deficient mice stimulated with LPS showed alleviative lung
pathological injury and inflammatory response. HDAC3 silence by
small interfering RNA (siRNA) significantly blocked the activation
of cyclic GMP-AMP synthase (cGAS)/stimulator of interferon genes
(STING) pathway while HDAC3 overexpression by adenovirus
transfection significantly promoted the activation of cGAS/STING
pathway and aggravated pyroptosis in LPS-induced macrophage.
However, HDAC3 silence or overexpression at baseline showed no
effects on the level of mitochondrial DNA (mt-DNA) and the
activation of cGAS/STING pathway. But HDAC3 at baseline could
change the mRNA and protein levels of cGAS. Additionally, autophagy
or proteasome inhibition in LPS-induced macrophages transfected
with Hdac3 siRNA did not affect the protein level of cGAS.
Mechanistically, cGAS was a direct target gene of miR-4767 in
macrophage. LPS could recruit HDAC3 and H3K9Ac to the miR-4767 gene
promoter, which repressed the expression of miR-4767 by decreasing
histone acetylation of the miR-4767 gene promoter. To this end,
intratracheal administration of liposomes loaded with
Hdac3siRNA prevented mice from LPS-induced lung injury and
inflammation. Taken together, our findings demonstrated that HDAC3
played a pivotal role in mediating pyroptosis in macrophage and ALI
by activating cGAS/STING pathway through decreasing histone
acetylation of the miR-4767 gene promoter. Targeting HDAC3 in
macrophage may provide a new therapeutic target for the prevention
of LPS-induced ALI.
An Investigation of the Implementation of the Dual Vocational Education and Training: Field Studies in Six Vocational Colleges
Authors: Van BUI Thi Thanh 
Published: February 2023

Abstract
In recent years, Vietnamese Vocational Education and Training (VET)system has been employed with numerous reforms, especially the adoption of the German Dual VET. In the current context of globalization, Vietnam now requires high-quality manpower more than ever. By far, most VET-related studies have provided overall scenarios on Vietnam’s VET but there has been no real investigation at vocational institutions. This research aimed to answer two questions: (1) To what extent does the number of affiliate enterprises affect the effectiveness of Vietnamese Dual VET?, (2) How effective is on-the-job (OJT) training curriculum of the Dual VET system toward enhancing the qualification of Vietnamese workers? Targeting the key industrial zones in all three regions of Vietnam where industries are concentrated and attract many workers, this study conducted an investigation at six vocational colleges in five provinces that have been implementing the dual model. Under the mixed method approach, the data were triangulated in the convergence framework of analysis. By conducting online questionnaires with570 vocational students and 17 semi-structured interviews with vocational educators and enterprises' managers, this research explored the real status of the job-oriented training in sampled vocational colleges.The most frequent form of questions used for the questionnaire of this study was the Likert scale, which resulted in ordinal data. As for the large number of scaled questions, the researcher decided to analyze the internal consistency (reliability) of the scales by calculating Cronbach’s alpha coeficient. The α coefficients of the three scales used in the research, including “Facilities & Equipment of the college”, “Business Association Activities”, and “OJT evaluation” are 0.853, 0.940, and 0.925, respectively, which are all excellent levels of internal consistency. The following findings were discovered: (i) most of the sampled vocational students did not have positive feedback toward OJT; (ii) a higher number of partner enterprises indicates the better quality of OJT; and (iii) a high-quality OJT is a key factor in facilitating the sustainable development of the Vietnamese Dual VET system. From those the author raised some policy recommendations to move forward with the improvement of the dual VET quality and enhancement of the workforce.
3D Imaging Reconstruction Can Recognize Functional Tissue and Predict Liver Failure.
Authors: Vicente Emilio , Quijano Yolanda , Duran Hipolito , Diaz Eduardo , Fabra Isabel , Malave Luis , Ruiz Pablo , Pizzuti Giada , Naldini Chiara , De Nobili Giovanni , Caruso Riccardo , Ferri Valentina 
Published: February 2023

Abstract
Background: The aim of this study is to evaluate the future liver remnant after major hepatectomy with 3D modelling treated, respectively, with associated liver partition and portal vein ligation for staged hepatectomy (ALPPS) and portal vein embolization (PVE).
Methods:
Patients submitted to ALPPS at our centre were compared with a group of patents submitted to the PVE followed by major hepatectomy. 3D reconstruction was realised before and after surgical treatment, and anatomic future liver remnant (FRL) and functional future liver remnant (FRFx) were calculated and compared with clinical outcomes.
Results:
From May 2011 to October 2019, 11 ALPPS procedures were realised at Sanchinarro University Hospital, Madrid. They were compared to a control group composed of 13 patients who underwent PVE followed by hepatectomy. The increase of anatomical FRL (iFRL) was superior in the ALPPS group (120%) with respect to the PVE group (73%) (p=0,041), while the increase of functional FRFX (iFRFx) was 35% in the ALLPS group and 46% in the PVE group (p>0,05), showing no difference in the two groups. PHLF grade B and C occurred in 6 patients. The ROC curve showed a better AUC for FRFxVpost (74%) with respect to FRLVpost (54%) in prediction PHLF>B.
Conclusion: The 3D reconstruction model can allow optimal surgical planning, and through the use of specific algorithms, can contribute to differential functioning liver parenchyma of the FLR.
Elbow hemiarthroplasty with a 3D-printed megaprosthesis for distal humeral bone defects after tumor excision: A cases report
Authors: Zhu Yingkang , Gong Shuo , Dai Jin , Zhou Lei 
Published: February 2023

Abstract
Background: The distal humerus is a rare site for primary and metastatic bone tumors. The therapeutic reconstruction modality of bone defects after tumor resection around the elbow remains extremely challenging at present. Therefore, due to the scarcity of cases and lack of standardized surgical strategies, it is often difficult for surgeons to choose the right choice. The application of 3D-printed megaprosthesis with hemiarthroplasty for the treatment of the distal humerus after tumor resection can be a very effective option.
Case presentation: We present a clinical case of a 3D-printed distal humeral megaprosthesis for treatment of bone defects caused by metastatic bone tumors. The patient suffered a pathological fracture due to metastatic tumor invading the distal humerus, which affected daily life because of significantly limited pain activity. Preoperative evaluation was aggressively performed, and the decision was made to distal humeral hemi-arthroplasty (DHH) after wide resection of the tumor segment bone. Processing of the Digital Imaging and Communications in Medicine (DICOM) data from CT scans performed after mirror conversion using CT data of the contralateral humerus, we designed a 3D-printed distal humeral megaprosthesis with hemiarthroplasty. After reconstruction of bone and surrounding soft tissue by the 3D-printed megaprosthesis combined with the LARS ligament and regular follow-up for 12 months, the patient had an MSTS-93 score of 29 and an MEPs of 100, which reached a good level, and the patient was fully competent in normal daily activities.
Conclusions: Our results show that the 3D-printed modular megaprosthesis with hemiarthroplasty is a very effective option for cases large elbow bone defects due to primary bone tumors or metastatic disease. However, careful preoperative preparation is required for the best outcome. Careful preoperative preparation and long-term follow-up are essential for the best outcome.
Gall-Forming Nematode, Anguina Woodi (Nematoda: Anguinidae), Gall Midge Mitodiplosis Graminis (Diptera: Cecidomyiidae) And Chalcid Wasp, (Hymenoptera: Eurytomidae) On Dune Grass From the Western Cape, South Africa
Authors: Chauke Tinyiko , Subbotin S. A. , Maseko Z. , Mamabolo M. M , Swart A. , Malatji Petunia 
Published: February 2023

Abstract
In 2021/2022, galls formed by a nematode, Anguina woodi were found on the stems, leaves and leaf sheaths of dune grass, Ehrharta villosa Schult. var. villosa on Milnerton Beach, Blouberg Beach and Langebaan Nature Reserve, Western Cape Province, South Africa. These galls were spongy in texture, deep purple to blackish in color and non-pedunculate. They were found in clusters, but also as single entities. Larger, harder galls varying from beige to dark brown in colour, apparently caused by insects, were also found on the stems of dune grass at Blouberg Beach and Langebaan Nature Reserve. Some nematode galls were found immediately next to, or on top of the insect galls. Those found on top of insect galls seemed to be harder and drier than those found on stems not infected by insect galls. The co-infection of insects and anguinid nematodes has not been reported from the current study areas and was thus included in the present study. Both molecular and morphological studies were conducted on the nematodes and insects leading to that a host specific, gall forming nematode was identified from all three localities; the gall-forming insects were identified morphologically and molecularly to family Eurytomidae (Hymenoptera) and the gall midge Mitodiplosis graminis Kieffer (Diptera: Cecidomyiidae). No insect galls were found on dune grass from Milnerton Beach. The paper includes speculations on a probable association between nematodes and insects
Porous Bleed Boundary Conditions for Supersonic Flows with &amp; without Shock-Boundary Layer Interaction
Authors: Giehler Julian , Grenson Pierre , Bur Reynald 
Published: February 2023

Abstract
In this paper, several existing porous bleed models are implemented into the in-house RANS-solver elsA, and their performance are evaluated on two different flow cases. Reference simulations are performed to evaluate the accuracy of the applied bleed models and to show the lack of modeling different plate geometries. The models significantly differ in predicting the bleed mass flux along the plate. For the turbulent supersonic boundary layer bleeding, the reference simulations demonstrate a hole diameter influence on the flow, but none of the models considers this effect. The model of Slater fits well with the reference simulation by applying it to its extracted data. However, applying the bleed models as a boundary condition results in more significant deviations from the reference simulation, leading to an overestimation of the wall shear stress and fuller boundary layer profiles downstream of the bleed region. Additionally, in the second flow case, including the shock-boundary layer interaction, the effect of the porous bleed is overestimated. No model is found suitable for a shock-boundary layer interaction control, and essential parameters such as the bleed hole diameter are not considered.
Hydrological effects on Rainfall-Induced Slope instability: A Case Study
Authors: Gidon J. S , Sahoo S 
Published: February 2023

Abstract

Real-time monitoring of the slope will give the actual slope assessment and according to the data received the slope behavior can be analyzed. Monitoring the soil slope behavior is essential to understand the slope section's failure mechanism. According to NDMA (National Disaster Management Authority) report, 2019–2020, 15% of the landmass in India is prone to landslides. The measurement of pore water pressure and site displacement for different rainfall intensities can be obtained. Measuring the performance of the slope and hydrological conditions will give more understanding of the underlying problem of slope behavior. Proper instrumentation during real-time monitoring of the slope can provide a proper understanding of the failure mechanism of the soil slope and the development of the failure surface due to seepage of rainwater. Reduction of the catastrophic effect can be achieved by having a proper insight into the slope behavior. The positions of tensiometers placed at different levels of the slope shows the significance of pore water pressure developed not only due to presence of groundwater table but that developed during rainfall period. Finite element (FE) analysis can be performed for validation of the results obtained from the field study. The potential slip surface of the slope determined from the analysis shows the location at which the slope failure can potentially occur. The simulation shows that the FE based study present here can be used in other slope study as well. A reliable and sustainable slope stabilization technique has been developed to tackle slope instability due to rainfall in the study area using the field data and the FE analysis. A threshold level can be identified by the system providing advanced warning criteria for the area.
Yttrium decorated fullerene C30 as potential hydrogen storage material: Perspectives from DFT simulations
Authors: Paul Debolina , Mane Pratap , Sarkar Utpal , Chakraborty Brahmananda 
Published: February 2023

Abstract
Using the density functional theory method, hydrogen storage capacity for Yttrium doped fullerene has been studied. Bonding of Y atom with that of C30 is due to the charge transfer taking place from the d-orbital of the Y atom to the 2p-orbital of the C atom of C30. It has been predicted that a single Y atom can adsorb 7 hydrogen molecules, whose binding energy falls within the range as suggested by the U.S. Department of Energy (DOE). Interaction of hydrogen on the metal is because of the Kubas interaction where charge donation occurs from the metal d-orbital to the hydrogen 1s-orbital and there is also back donation as a result the hydrogen adsorption energy is more than physisorption. However, H atoms in the H2 molecule is not getting dissociated, only a small elongation of H-H bond in the H2 molecule is observed. The gravimetric weight percentage for 5 Y atoms loaded fullerene C30, with each Y atom adsorbing 7 H2 molecules is recorded to be 8.060%, higher than the limit of 6.5% by DOE. These findings suggest Y doped fullerene C30 may be considered as a potential candidate for hydrogen storage devices.
Synthesis of N-monosubstituted ureas in a mixture of choline chloride and Aluminium nitrate as a green and selective process
Authors: jafari mohammadreza , Darvishi Atefeh , Heydari akbar 
Published: February 2023

Abstract
The reaction of benzyl alcohol or benzyl halide with urea in a mixture of choline chloride and aluminum nitrate ionic liquid as a solvent resulted in the formation of N-monosubstituted urea products. This reaction was achieved good to excellent yields for a number of derivatives of benzyl alcohols and benzyl halides under the reaction conditions. Since the resulting products were so polar and insoluble in water, they were easily separated from the reaction medium by adding water to the reaction mixture. Therefor such ionic liquid provided a facile and selective route for the synthesis of pharmaceutically and industrially substantial compounds.
Error analysis for calculation methods of shear capacity of FRP reinforced concrete beams without web reinforcement based on database
Authors: Wang Tao , Fan Xiangqian , Gao Changsheng , Qu Chiyu , Liu Jueding 
Published: February 2023

Abstract
The existing calculation methods of shear capacity of FRP reinforced concrete beams without web reinforcement are inconsistent in considering the factors of shear capacity, and there are different degrees of conservative calculation. Based on the experimental data of shear capacity in published literatures at home and abroad, a database with a sample size of 461 was established in this paper. The effects of six factors, such as the compressive strength of concrete, the width of the beam, the effective depth of the beam, the shear span to depth ratio, the reinforcement ratio of FRP bars and the elastic modulus of FRP bar, on shear capacity were analyzed comprehensively, and the prediction effect of each calculation method was evaluated. The results showed that the influence of each calculation method on the size effect and shear span to depth ratio was not consistent, and there were different degrees of conservative calculation, there was a good correlation between the factors and the shear capacity; among the design provisions recommended by different countries, the CSA/CAN-S806-2012 has a more accurate prediction effect, and the ACI440.1R-2015 has the most conservative prediction effect; among the calculation models provided by scholars, the prediction effect of Ahmed-2021 is more accurate, and that of Alam-2013 is the most conservative. The research results provided the certain referential value for developing the calculation method of shear capacity of FRP reinforced concrete beams without web reinforcement.