bluuebunny/crossref_metadata_2025_split huggingface dataset:
Sperm DNA integrity does play a crucial role for embryo development after ICSI, notably when good-quality oocytes from young donors are used
Authors: Ribas-Maynou Jordi , Novo Sergi , Torres Marc , Salas-Huetos Albert , Rovira Sergi , Antich Marta , Yeste Marc 
Published: December 2022

AbstractBased on the inconsistent literature published thus far involving infertile patients, whether intracytoplasmic sperm injection (ICSI) allows overcoming total fertilization failure due to sperm DNA fragmentation is still unclear. Related to this, female factors, which may have a significant impact on assisted reproduction outcomes, can mask male infertility. In this scenario, evaluating ICSI outcomes following cycles using healthy donor gametes could shed light on this realm, as it would avoid the influence of (un)known confounding factors present in infertile individuals. The present work, therefore, aimed to address whether single- and double-stranded sperm DNA fragmentation leads to impaired ICSI outcomes in double gamete donation cycles. The study also compared these double-gamete donation cycles to cycles in which only sperm were donated and oocytes were obtained from infertile patients. Two cohorts were included: (a) the Donor-Donor (DD) cohort, which included 27 semen donor samples used in 49 ICSI cycles with young healthy oocyte donors; and (b) the Donor-Infertile (DI) cohort, which involved 34 semen donor samples used in 57 ICSI cycles with oocytes from patients. Single- and double-stranded sperm DNA breaks were determined with alkaline and neutral Comet assays, respectively; ICSI was conducted following standard protocols and embryos were monitored through time-lapse microscopy. In the DD cohort, the percentage of sperm with high overall DNA damage correlated with fertilization rates (Rs = − 0.666;P < 0.001) and with the percentage of blastocysts per injected oocyte (Rs = − 0.414;P = 0.040). In addition, sperm DNA damage delayed the first embryo division (Rs = 0.421;P = 0.036), and development from the 8-cell to the morula stage (Rs = 0.424;P = 0.034). In contrast, double-stranded DNA breaks had no effect in this cohort. As far as the DI cohort is concerned, while overall sperm DNA damage was not found to be correlated to fertilization or blastocyst rates, pronuclei formation following ICSI was delayed when the incidence of double-stranded DNA breaks was high (Rs = 0.485;P = 0.005). In conclusion, this study, which is the first involving double donation cycles (i.e., a donor-donor cohort), supports that sperm DNA damage has a detrimental impact on fertilization rates after ICSI, and delays embryo development. Moreover, the use of oocytes from infertile individuals is suggested to hide the male-factor effect.
Temporary establishment of bacteria from indoor plant leaves and soil on human skin
Authors: Mhuireach Gwynne Á. , Fahimipour Ashkaan K. , Vandegrift Roo , Muscarella Mario E. , Hickey Roxana , Bateman Ashley C. , Van Den Wymelenberg Kevin G. , Bohannan Brendan J. M. 
Published: December 2022

AbstractBackgroundPlants are found in a large percentage of indoor environments, yet the potential for bacteria associated with indoor plant leaves and soil to colonize human skin remains unclear. We report results of experiments in a controlled climate chamber to characterize bacterial communities inhabiting the substrates and leaves of five indoor plant species, and quantify microbial transfer dynamics and residence times on human skin following simulated touch contact events. Controlled bacterial propagule transfer events with soil and leaf donors were applied to the arms of human occupants and repeatedly measured over a 24-h period using 16S rRNA gene amplicon sequencing.ResultsSubstrate samples had greater biomass and alpha diversity compared to leaves and baseline skin bacterial communities, as well as dissimilar taxonomic compositions. Despite these differences in donor community diversity and biomass, we observed repeatable patterns in the dynamics of transfer events. Recipient human skin bacterial communities increased in alpha diversity and became more similar to donor communities, an effect which, for soil contact only, persisted for at least 24 h. Washing with soap and water effectively returned communities to their pre-perturbed state, although some abundant soil taxa resisted removal through washing.ConclusionsThis study represents an initial characterization of bacterial relationships between humans and indoor plants, which represent a potentially valuable element of biodiversity in the built environment. Although environmental microbiota are unlikely to permanently colonize skin following a single contact event, repeated or continuous exposures to indoor biodiversity may be increasingly relevant for the functioning and diversity of the human microbiome as urbanization continues.
Late-onset rheumatoid arthritis registry study, LORIS study: study protocol and design
Authors: Kojima Masayo , Kawahito Yutaka , Sugihara Takahiko , Kojima Toshihisa , Harada Ryozo , Hirata Shintaro , Hashimoto Motomu , Hidaka Toshihiko , Ishikawa Hajime , Ito Hiromu , Kishimoto Mitsumasa , Kaneko Yuko , Matsui Kazuo , Matsui Toshihiro , Matsushita Isao , Morinobu Akio , Nishida Keiichiro , Tanaka Eiichi , Abe Asami , Ishitoku Michinori , Asai Shuji , Kida Takashi , Onishi Akira , Takanashi Satoshi , Harigai Masayoshi 
Published: December 2022

Abstract
Background
Although drug treatment strategies for rheumatoid arthritis (RA) are relatively well established, there is a paucity of evidence on the treatment in older patients. The purpose of this study is to build a registry for late-onset RA (LORA), which is expected to increase rapidly worldwide. In addition, we aim to propose optimal treatment strategies according to the patient background including frailty, thereby contributing to improving the quality of treatment and daily living in patients with RA.

Methods/design
The LORIS (Late-onset Rheumatoid Arthritis Registry) Study is a prospective nation-wide multicenter observational study of patients with LORA. The inclusion criteria were patients aged ≥ 65 years at onset, meeting 2010 ACR/EULAR classification criteria for RA, and starting either any disease-modifying antirheumatic drugs (DMARDs) in a DMARD-naïve patient or the first biologic/targeted synthetic DMARDs during the study period. Enrollment was started on 11 January, 2022 and will be closed on 31 December, 2023. Patients will undergo a comprehensive baseline assessment including clinical data, medication, cognitive and physical function, psychosocial factors, and frailty. Data will be collected at baseline, Month 3, 6, 12, 18, 24, 36, and summarized descriptively. The factors associated with adverse events and achieving remission will be determined.

Discussion
A multi-disciplinary panel including patients, rheumatologists, and geriatric specialists will discuss the results and build a consensus regarding the treatment goals of LORA. We expect to provide a broad range of information for evidence-based shared decision making in the treatment of LORA.
Study registration: Registered at the UMIN registry (UMIN000046086) on 1 January 2022.
Efficacy of low-dose intravenous dexamethasone for prolongation of analgesia in supraclavicular block
Authors: Solanki Nilesh M. , Maheshwari Khevana P. 
Published: December 2022

Abstract
Background
Supraclavicular block is a common technique for analgesia for upper limb surgeries. Because of short duration of analgesia with bupivacaine alone various additive have been used to prolong the action of bupivacaine. We conducted a prospective, randomized, double blind controlled trial. All patients were assessed for onset and duration of sensory and motor block, and postoperative pain relief.
The present study was aimed to evaluate the efficacy of intravenous dexamethasone with supraclavicular block for prolongation of analgesia in patients posted for upper limb surgeries.

Results
Fifty patients belonging to American Society of Anesthesiologist physical status I–II were divided into two groups (25 in each). A mixture of bupivacaine 0.5% (15 ml) + lignocaine-adrenaline 1.5% (15 ml) were given in both the groups by ultrasound guided supraclavicular block and 2 ml of IV normal saline in group C and 2 ml (8 mg) of IV dexamethasone in group D were given. The mean onset time of sensory and motor block was 8.68 ± 1.81 min and 11.12 ± 1.58 min in group D and 11 ± 2.34 min and 13.2 ± 1.65 min in group C respectively. The duration of sensory and motor block in group D (843.6 ± 62.56 and 576.8 ± 70.40) min were prolonged than to group C (281.28 ± 22.42 and 204.2 ± 12.96) min where P < 0.0001.The duration of analgesia in group D was (895.6 ± 43.98 min) as compared to group C (324.4 ± 27.36 min). At the end of 4 h, the difference of the mean VAS score was statistically significant in both the groups (P < 0.05). There was no statistically significant difference in heart rate and systolic blood pressure in both the groups.

Conclusions
Intravenous dexamethasone significantly prolong the duration of analgesia in supraclavicular block without the side effects.
TMPRSS6 rs855791 polymorphism is associated with iron deficiency in a cohort of Sri Lankan pregnant women
Authors: Abeywickrama Halinne Lokuge Thilakshi Chamanika , Rabindrakumar Miruna Sudharshani Kalaimani , Pathira Kankanamge Lakindu Samaranayake , Thoradeniya Tharanga , Galhena Gayani Harendra 
Published: December 2022

Abstract
Background
Hepcidin is the key regulator of systemic iron homeostasis and is downregulated by matriptase 2 (MT2), a protease encoded by TMPRSS6 gene. In the presence of low iron levels, MT2 cleaves membrane-bound hemojuvelin (HJV), causing a negative regulation of hepcidin at the gene level, and restores iron balance. rs855791T > C, a missense variant in the catalytic domain of MT2, causes valine to alanine change at 736 position. The current study aimed to investigate the association of TMPRSS6 rs855791 on iron status among a cohort of pregnant women in Sri Lanka and to predict the possible molecular mechanisms.

Methods
The study was conducted among 73 pregnant women at ≤ 12 weeks of gestation. Iron deficiency was defined as serum ferritin < 30 μg/L after adjusting for inflammation. rs855791 was genotyped with a PCR–RFLP, and its association with iron deficiency was analyzed using binary logistic regression. Docking of HJV with MT2 protein encoded by the two rs855791 alleles was undertaken in silico to predict the molecular mechanism of the observed associations.

Results
The majority of the study population (70%) were iron deficient. Among the subjects, T allele was prevalent in the iron deficient group with a frequency of 61.8%, with a nearly twofold enhanced risk for iron deficiency (OR = 2.566, 95%CI; P = 0.011). For TT genotype, the risk of iron deficiency was nearly sixfold (OR = 5.867; 95%CI; P = 0.023). According to the in silico analysis, MT2 736A and HJV complex is more stable with an interface energy of − 7.934 kJ/mol compared to the MT2 736 V and HJV complex which generates an interface energy of − 4.689 kJ/mol.

Conclusion
The current study suggests that the iron regulatory effect of rs855791 of TMPRSS6 is brought about by the differences in thermodynamic stability of the two protein complexes made by MT2 and HJV proteins. The prevalence of iron deficiency observed among Sri Lankan pregnant women may be an interplay between the prevalence of rs855791 T allele and the low dietary iron intake.
Frequency of gastrointestinal manifestations among infants with cow’s milk protein allergy, Egypt
Authors: El-Asheer Osama M. , El-Gazzar Amira F. , Zakaria Caroline M. , Hassanein Farouk A. , Mohamed Khalil A. 
Published: December 2022

Abstract
Background
Cow’s milk is known to be the most frequent food allergen in infants in the first years of life. Eliminating cow milk protein from diets and reintroducing it with a challenge test are the main methods for sure diagnosis. Cow’s Milk-related Symptom Score (CoMiSS) has been developed for primary health care providers to improve their knowledge about CMPA.

Objective
This study was conducted to prescribe clinical presentation of cow’s milk allergy among infants.

Methods
A cross-sectional study was conducted at Gastroenterology and Nutrition Unit, University Children Hospital, from the 1st of January 2020 to the 31st of December 2020. Forty infants, complaining of recurrent or persistent gastrointestinal manifestations, were included in our study (28 males and 12 females). Those infants were subjected to detailed medical and social patient history, comprehensive physical exam, and CoMiSS scoring as an awareness tool to suspect underlying cow milk allergy. Infants with a CoMiSS of more than 12 points and positive elimination rechallenge test were regarded as eligible to be part of our study.

Results
This study showed that the most frequently occurring gastrointestinal manifestations associated with CMPA were regurgitation (92%), diarrhea (80%), colic (75%), vomiting (67.5%), and lastly constipation which represents only (5%).

Conclusion
Regurgitation and diarrhea are the commonest presentations for infants proved to have cow milk allergy with CoMiSS score and elimination rechallenge test, where constipation is the least common presentation for those infants.
Response to Surgery Assessments for Sparing Radioiodine Remnant Ablation in Intermediate-Risk Papillary Thyroid Cancer
Authors: Zhang Xin , Liu Jie-Rui , Mu Zhuan-Zhuan , Cheng Xin-Qi , Lin Yan-Song 
Published: December 2022

Abstract

Context
Using response to surgery when tailoring radioiodine (RAI) therapy for papillary thyroid cancer (PTC) is valued but lacks prospective validation.

Objective
To spare RAI thyroid remnant ablation among patients with intermediate-risk PTCs using 3-tiered assessments with response to surgery highlighted, in addition to the risk of the recurrence stratification and TNM staging.

Methods
Patients with no evidence of disease (NED) identified as excellent response (ER) or indeterminate response (IDR) to surgery were spared from RAI thyroid remnant ablation after informed consent and prospectively enrolled under active surveillance. Those involved in other trials or without sufficient follow-up data were excluded. Dynamic responses were followed and compared longitudinally. The main outcome measures were NED presenting as durable ER or IDR for over 12 months.

Results
Of the enrolled 215 patients, 47.4% (102/215) ER and 52.6% (113/215) IDR were identified regarding RAI decision-making. After a median of 23.6 (interquartile range 13.8-31.6) months, the share of ER increased to 82.8% (178/215) and IDR decreased to 16.3% (35/215), with 85 patients shifting from IDR to ER over time, only 0.5% (1/215) structural incomplete response and 0.5% (1/215) biochemical incomplete response observed. Successful remnant ablation was observed in 27.7% (26/94) of the patients completing 2 diagnostic whole-body scans after a median interval of 13.0 months, indicating a theranostic effect. In the 173 patients followed for over 12 months, the NED rate did not differ between ER and IDR subgroups (100% vs 97.9%, P = .20).

Conclusion
Through the 3-tiered assessments with response to surgery highlighted, postoperative ER and IDR spared from RAI remnant ablation may indicate similar favorable responses in intermediate-risk patients with PTC during 23.6 months of follow-up.
Intestinal microbiota and obesity
Authors: Zuziak Patrycja , Ilnicka Natalia , Abod Leila , Matyja Daria , Sadlik Maria 
Published: December 2022

Introduction and purpose:
The growing number of obese people is one of the greatest problems of modern medicine. It is estimated that every 10th person is obese. Recent studies also suggest that the gut microbiome may contribute to the pathogenesis of obesity.
Description of the state of knowledge:
Microbiota is named a super organ that has the ability to evolve in response to changing conditions within the host organism. It is named so because it is made up of over 100,000 billion cells that belong to different groups of organisms. Microbiota consists of bacteria, fungi, protists, viruses and archaea. This taxonomically diverse collective plays a vital role in maintaining the homeostasis of the human body. A well-balanced intestinal flora protects the digestive tract from colonization by pathogenic microorganisms, it is also important in maintaining the integrity of the intestinal walls, is involved in the synthesis of certain vitamins and plays a role in nourishing mucosa cells. Moreover, it is known that the microflora can influence body weight, insulin sensitivity, and metabolism of certain sugars and lipids. Disturbances in the composition of the microbiome (dysbiosis) lead to an increased absorption of short-chain fatty acids, which leads to an increase in endogenous YY peptide synthesis. This protein slows down the intestinal transit and indirectly leads to increased absorption of nutrients. This predisposes to the development of obesity. The use of prebiotics, probiotics and some antibiotics reduces intestinal dysbiosis. For obese patients, it is associated with reduction of insulin resistance and decrease in blood glucose level.
Conclusions:
In the light of the available studies, it can be concluded that intestinal dysbiosis may play a role in the pathogenesis of obesity. Unfortunately, the practical application of this knowledge is not yet well documented. Therefore this topic requires further experiments and analysis.
Penetapan kadar alkohol pada kosmetik menggunakan metode kromatografi gas
Authors: Albab Farah Qisth , Mahfudh Nurkhasanah 
Published: December 2022

Alkohol merupakan bahan yang banyak digunakan di farmasi, salah satunya kosmetik. Â Penggunaan alkohol pada kosmetik biasanya memiliki fungsi sebagai pelarut, astrigent, desinfektan, mengurangi tegangan permukaan, dan meningkatkan daya pembersih. Â Penggunaan alkohol di kosmetik menurut Majelis Ulama Indonesia diperbolehkan asalkan tidak berasal dari industri khamr, namun sekarang banyak kosmetik yang terdapat label free alcohol. Tujuan penelitian ini adalah untuk mengidentifikasi kandungan alkohol pada produk kosmetik yang mengklaim free alcoholÂ dan mengetahui kadar alkohol pada kosmetik. Metode penelitian dilakukan mengunakan teknik obsevasional survey, non probability berupa simple random sampling pada kosmetik di swalayan. Salah satu validasi metode analisa yaitu uji kesesuaian sistem dilakukan sebelum analis sampel untuk memastikan bahwa instrumen dapat menghasilkan data yang dapat diterima yaitu nilai tR sampel untuk mengetahui mengandung alkohol atau tidak. Analisis kuantitatif dilakukan dengan rasio AUC etanol-isopropanol dengan kadar dan dibuat regresi linear. Hasil uji kesesuaian sistem dengan parameter RSD tR 0,2231%; TF 0,9568; N 2872,5318; dan Rs 1,874 memenuhi syarat kecuali RSD AUC 10,0277% tidak memenuhi syarat sehingga sistem akan mengahasilkan data yang dapat diterima.Â Empat sampel dari 6 sampel kosmetik micellar water dan toner positif mengandung alkohol tidak sesuai dengan label pada kemasan yaitu produk kosmetik Wardah, Mustika Ratu, Pixy dan Pondâ€™s. Dua sampel negatif tidak mengandung alkohol adalah Nivea dan Himalaya. Range kadar alkohol yang terdapat pada kosmetik tersebut dari 2,48 x10-4% - 4,96 x10-4 %.
Emotion in Animated Films, Mieke Uhrig (ed.) (2018)
Authors: Yazdandoost Farzaneh 
Published: December 2022

Review of: Emotion in Animated Films, Mieke Uhrig (ed.) (2018)
New York: Routledge, 238 pp.,
ISBN 978-0-36758-477-1, p/bk, $48.95
Analisis Waste Alur Pelayanan Rawat Jalan RS. Mata Undaan Surabaya Sebagai Upaya Peningkatan Capaian Waktu Tunggu
Authors: Damayanti Vinna Amalia Resi , Ernawaty Ernawaty 
Published: December 2022

Rumah Sakit Mata Undaan memiliki Indikator wajib yang salah satunya adalah waktu tunggu pelayanan yaitu di rawat jalan. Indikator pada waktu tunggu pelayanan di rawat jalan yaitu (≤ 60 menit) dengan target 100%. Pada tahun 2017 sampai dengan tahun 2019, capaian indikator waktu tunggu pelayanan rawat jalan sebesar 44,8%. Penelitian ini bertujuan untuk menyusun rekomendasi untuk meningkatan capaian waktu tunggu rawat jalan khusus dan rawat jalan umum RS Mata Undaan Surabaya dengan menganalisis waste yang terjadi. Penelitian ini merupakan penelitian deskriptif. Desain penelitian yang digunakan adalah observasional dengan pendekatan cross sectional study dengan sampel petugas di Instalasi rawat jalan dan pasien yang berobat di Instalasi rawat jalan pada periode Februari-Maret 2020. Hasil penelitian menunjukkan bahwa terdapat 8 jenis waste yang terjadi di setiap proses pelayanan yang dilalui oleh pasien mulai dari pendaftaran sampai dengan mengambil obat di farmasi yang didapatkan dari hasil observasi waktu tunggu pelayanan berupa hasil stopwatch dengan satuan menit per detik dengan melihat perbandingan antara nilai standar deviasi dengan mean. Jika nilai standar deviasi > dari nilai mean, maka dinyatakan sebagai pemborosan (waste). 8 jenis pemborosan (waste) tersebut selanjutnya diklasifikasikan menjadi 3 (tiga) kategori waste yang telah peneliti temukan, diantaranya terdapat 4 (empat) tergolong waiting, 3 (tiga) yang tergolong unappropriate processing, dan 1 (satu) yang termasuk transportation. Rekomendasi yang dapat diberikan adalah melakukan penjadwalan ulang bagi dokter, dilakukannya perencanaan E-rekam medis untuk penyimpanan berkas pasien, serta menjaga kebersihan dan pemeliharaan rutin disetiap fasilitas pendukung yang ada di Instalasi rawat jalan. Kata Kunci : Alur Pelayanan, Instalasi Rawat Jalan, Waste, Waktu Tunggu
Female Counter-Curation: A Case Study of Polish Napoleonic War Reenactment
Authors: Tomann Juliane 
Published: December 2022

Abstract
Historical reenactment has developed from a niche phenomenon to a global one over the past couple of decades. As a popular pastime it sparks enthusiasm and curiosity for past lifeworlds among the reenactors themselves as well as among the audiences. With its focus on restaging battles and combat, the reenactment environment is a predominantly male one. This article examines female practices of reenactment in a Polish reenactment group. It aims to not only add to the existing body of literature on female reenactors, but to further pinpoint the extent to which the concept of curation helps expand and deepen the understanding of reenactment practices both in general and with regard to female participation. The focus is on female-to-male cross-dressing, interpreted as an ambiguous practice which allows for both the deconstruction of normative gender performances and their stabilization.
Public History in Russia: The Past, the Present, and (Thoughts About) the Future
Authors: Zavadski Andrei , Dubina Vera , Isaev Egor , Kolesnik Alexandra , Lajus Julia , Suverina Katerina 
Published: December 2022

Abstract
This discussion’s participants – all public historians working on Russia, albeit from different disciplinary backgrounds and with different areas of expertise – speak about the past and the present of (public) history in the country, and touch upon possible futures. Beginning with an acknowledgment of the immense interest in historical knowledge that characterized the 1990s, the conversation goes on to examine the rise of the official historical politics in Putin’s Russia and their impact on historical science, memory work, and public engagement with the past more broadly. These developments contextualize the establishment of the first public history programs at Russian universities in the early 2010s, discussed here both in their specificities and compared to other countries. At the heart of the conversation is the war of aggression that Russia launched against Ukraine in February 2022. The participants of the discussion see it as a caesura, while at the same highlighting continuities in the regime’s historical politics before and after the invasion. Issues of postcolonialism and decolonization are also raised, as well as the question of (public) historians’ responsibility for the ongoing tragedy.