bluuebunny/crossref_metadata_2025_split huggingface dataset:
Anserine is expressed in human cardiac and skeletal muscles
Authors: de Souza Gonçalves Lívia , Pereira Wagner Ribeiro , da Silva Rafael Pires , Yamaguchi Guilherme Carvalho , Carvalho Victor Henrique , Vargas Bianca Scigliano , Jensen Leonardo , de Medeiros Marisa Helena Gennari , Roschel Hamilton , Artioli Guilherme Giannini 
Published: September 2023

AbstractWe evaluated whether anserine, a methylated analog of the dipeptide carnosine, is present in the cardiac and skeletal muscles of humans and whether the CARNMT1 gene, which encodes the anserine synthesizing enzyme carnosine‐N‐methyltransferase, is expressed in human skeletal muscle. We found that anserine is present at low concentrations (low micromolar range) in both cardiac and skeletal muscles, and that anserine content in skeletal muscle is ~15 times higher than in cardiac muscle (cardiac muscle: 10.1 ± 13.4 μmol·kg−1 of dry muscle, n = 12; skeletal muscle: 158.1 ± 68.5 μmol·kg−1 of dry muscle, n = 11, p < 0.0001). Anserine content in the heart was highly variable between individuals, ranging from 1.4 to 45.4 μmol·kg−1 of dry muscle, but anserine content was not associated with sex, age, or body mass. We also showed that CARNMT1 gene is poorly expressed in skeletal muscle (n = 10). This is the first study to demonstrate that anserine is present in the ventricle of the human heart. The presence of anserine in human heart and the confirmation of its expression in human skeletal muscle open new avenues of investigation on the specific and differential physiological functions of histidine dipeptides in striated muscles.
LncRNA LINC00592 mediates the promoter methylation of WIF1 to promote the development of bladder cancer
Authors: Wu Tieqiu , Li Nannan , Wu Xinghui , Du Yongchao , Tang Zhiwang 
Published: September 2023

Abstract
Epigenetic alteration is a key feature that contributes to the progression of bladder cancer (BC) and long non-coding RNAs serve crucial role in the epigenetic modulation. This study was designed to explore the epigenetic regulation of LINC00592 in BC. LINC00592 expression in BC was examined. Then, LINC00592 was silenced in BC cell followed by cell behavior analyses using CCK-8, transwell, western blot, or flow cytometry. Potential downstream target of LINC00592 was explored using RNA pull-down assay and methylation of WIF1 was determined using methylated-specific PCR. In addition, WIF1 or/and LINC00592 were silenced in BC cells followed by cell behavior analyses to explore the regulation between them. Upregulation of LINC00592 was significantly detected in BC tissues and cells. In BC cells silencing LINC00592 suppressed the proliferation, migration, and epithelial-mesenchymal transitions (EMT), but enhanced apoptosis. Moreover, LINC00592 recruited DNMT1, DNMT3A, and DNMT3B to enhance WIF1 promoter methylation. In addition, WIF1 overexpression suppressed the proliferation, migration, as well as EMT, but enhanced apoptosis. Silencing WIF1 significantly attenuated the role of silencing LINC00592 in suppressing the proliferative, migratory, and EMT ability of BC cells, and increasing the apoptosis. LINC00592 promoted the growth and metastasis of BC via enhancing the promoter methylation of WIF1 and decreasing WIF1 transcription.
Baseline differences in metabolic profiles of patients with lung squamous cell carcinoma responding or not responding to treatment with nanoparticle albumin-bound paclitaxel (nab-paclitaxel)
Authors: Cao Peng , Zhang Qilin , Wu Sanlan , Sullivan Mitchell A. , Huang Yifei , Gong Weijing , Lv Yongning , Zhai Xuejia , Zhang Yu 
Published: September 2023

Background:
Nanoparticle albumin-bound paclitaxel (nab-paclitaxel) is a preparation widely used in chemotherapy for cancers. However, only some patients benefit from this treatment. Therefore, identifying which patients will respond to nab-paclitaxel therapy is crucial.

Methods:
A cohort of 32 patients with lung squamous cell carcinoma (LUSC) treated with nab-paclitaxel were enrolled in this study. Plasma samples were collected before chemotherapy and used to perform metabolomic and lipidomic analyses. Tumor response to two cycles of chemotherapy was evaluated. Metabolites differentially present among populations were screened and analyzed.

Results:
According to the RECIST criteria, one-third of patients had a significant response to nab-paclitaxel, whereas one-fifth showed no discernible benefit. According to the criteria of variable importance in projection >1 and fold change >2, we identified 61, 81 and 54 differential metabolites between the progressive disease (PD) vs partial response (PR), PD vs stable disease (SD), and SD vs PR groups, respectively. Moreover, we used three variation in logistic regression models and ROC diagnostic curves to identify optimal metabolites for stratifying patients with differing chemotherapeutic responses. The PD vs SD, SD vs PR, and PD vs PR groups were well separated on the basis of cis-9,10-epoxystearic acid/octapentaenoic acid (AUC 0.9330), salicyluric acid/DG (18:1/20:5/0:0) (AUC 1.0000) and D-glyceric acid/9,12-octadecadienoic acid (AUC 1.0000), respectively.

Conclusion:
The baseline metabolic profiles significantly differed between responder and non-responder patients with LUSC treated with nab-paclitaxel. These differential metabolites have the potential to predict the outcomes of patients with LUSC before chemotherapy.
Epidemiological characteristics of hereditary motor and sensory neuropathy in the Sumy region
Authors: Govbakh Iryna , Mishchenko Tamara 
Published: September 2023

The aim of the study was to investigate the characteristics of the spread of hereditary motor and sensory neuropathy type 1A in the Sumy region, in terms of administrative units and specific ethnic groups of the population.
Materials and methods. An epidemiological study of hereditary motor and sensory neuropathy (HMSN) type 1A in the Sumy region was conducted based on patient referrals to various healthcare facilities from 2014 to 2017. The prevalence of HMSN in the Sumy region was studied using an epidemiological approach. The prevalence rate of HMSN was calculated for different administrative units of the Sumy region, as well as for specific ethnic groups of the population.
Results. The study registered 67 patients with hereditary motor and sensory neuropathy type 1A in the Sumy region. The prevalence rate of hereditary motor and sensory neuropathy type 1A in the Sumy region was 5.96 per 100,000 population. Of the HMSN patients, 47.8 % (32 individuals) resided in urban areas, while 52.2 % (35 individuals) lived in rural areas. In terms of gender distribution, 59.7 % (40 individuals) were female, and 40.3 % (27 individuals) were male. Most patients were of Ukrainian ethnicity (77.6 %), followed by Russians (20.9 %). Belarusians accounted for 1.5 % of HMSN patients in the Sumy region. Among other ethnic groups present in the Sumy region, HMSN type 1A was not detected.
Conclusions. Hereditary motor and sensory neuropathy type 1A is unevenly distributed in the Sumy region. The heterogeneous distribution of the disease is primarily attributed to the characteristics of settlement in specific geographic regions, which have led to the formation of territorial, national, and religious isolates with the accumulation of genetically heterogeneous types in these populations, resulting in an increased genetic burden
Increasing the accuracy of handwriting text recognition in medical prescriptions with generative artificial intelligence
Authors: Yakovchuk Oleg , Vasin Maksym 
Published: September 2023

The object of the research is a system for recognizing handwritten text in medical prescriptions. The peculiarities of handwriting, the variety of calligraphy styles, as well as the specificity of medical prescriptions, create many problems and challenges for recognition algorithms, causing errors and reducing recognition accuracy.
The work presents a new system with additional components of post-processing the recognition results to increase the accuracy of the final results. An algorithm for combining words into lines and blocks is proposed, which makes it possible to group words while preserving contextual connections between them. Also, a generative neural network with a large language model is used to analyze the recognition result and correct possible errors. The results of the testing show an improvement in recognition accuracy by 0.13 %. Successful cases of generative artificial intelligence usage are analyzed, as well as examples of the results deterioration, that are related to grammatical errors in the initial input data.
The obtained results show the use of generative artificial intelligence as an additional step for processing the recognition results really can improve the accuracy of text recognition systems. The results of the study can be used for further experiments to improve recognition results in other tasks related to text recognition and in related fields.
Analysis of the possibilities of increasing the energy efficiency of absorption refrigeration appliances through the use of refrigerating accumulators
Authors: Berezovska Liudmyla , Titlov Oleksandr 
Published: September 2023

One of the most important tasks of modern society is the solution of environmental and energy problems in power engineering and, in particular, refrigeration technology. At the same time, in the field of artificial cooling systems, it is necessary to solve the problems of reducing the impact on both the ozone layer and the greenhouse effect. An effective approach here can be absorption refrigeration systems with a natural working fluid (water-ammonia solution), which does not adversely affect the environment. For the effective use of absorption refrigeration systems, it is necessary to solve the problems of increasing their energy efficiency, in particular, through the use of cold accumulators. Thus, the object of research is absorption-type cooling systems with cold accumulators.
The paper analyzes cold accumulators with different physical nature. It is shown that melting substances can be the most effective for solving problems of low-temperature cooling. An analysis of the thermal scheme of an absorption freezer of the «chest» type, which is the most problematic in terms of providing cooling modes at a level of (–18)–(–24) °С, was carried out. Optimization thermal calculations for typical absorption freezers up to 200 dm3 have been performed. It is shown that when the chamber is initially loaded with a product at an ambient temperature, the cooling capacity of the installed absorption refrigeration units is not enough – no more than 50 % of the required one. For an absorption freezer of the «chest» type, the most suitable cold-storage materials are a eutectic aqueous solution of sodium chloride or propylene glycol, since these solutions have the desired melting point of the order of –18 °C and a fairly high melting heat. The result of optimizing the weight and size characteristics of the internal volume of the absorption freezer is the following recommendations:
– the optimal size of wire baskets for placing products is 315´370´240 mm;
– the gaps between the basket and the cabinet wall, as well as between the baskets themselves, should be 10 mm to ensure normal convection conditions;
– it is not advisable to place fans inside the volume of the freezer at this stage, since the freezing time is reduced by a maximum of 30 %, but this results in additional heat generation, energy consumption and increases the shrinkage of the products stored in the chamber.
Analysis of genetic polymorphism in genes encoding cathelicidins from Vietnam indigenous yellow cattle breeds
Authors: Tuoi Do Thi , Ngoc Ta Hong , Anh Tran Thi Thuy , Lan Pham Doan , Phuc Do Thi , Nguyen Thi Hong Van 
Published: September 2023

Cathelicidins include antimicrobial peptides (AMPs) and are involved in the innate immune defense against infections in mammals. Polymorphisms in the DNA sequence of cathelicidin genes could be relevant to inherited variations of host innate immunity, adaptation, and pathogen resistance. This study aims to investigate the sequence polymorphism of cathelicidin genes including CATHL2 and CATHL4 from local indigenous yellow cattle breeds of Vietnam. Genomic DNA samples were extracted from 52 individuals collected from different cattle populations in Vietnam including Ha Giang, Thanh Hoa, Nghe An and Phu Yen. The CATHL2 and CATHL4 genes were amplified by PCR, following sequencing for identification of the single nucleotide polymorphisms (SNPs) and/or insertion-deletion (indel) DNA sequence variations. The copy number variations (CNVs) of the CATHL4 gene were determined by cloning, single strand conformation polymorphism (SSCP) and real-time PCR. The comparative analysis results showed that there are 13 SNPs detected in all sequences of CATHL2 gene including 9 SNPs in intron 2 and intron 3, and 4 missense substitutions in exon 1, 2, and 3. These SNPs do not affect the predicted tertiary structure of Bac5 encoded by this gene. For CATHL4, the results revealed 15 SNPs, 3 indels and 1 repeat variation as motif (TG)n(G)n/(AC)n(C)n. Among these variations, the deletion 46delC in exon 1 of CATHL4 was detected in a sample of the Ha Giang breed causing a truncated polypeptide as predicted. The average copy number of CATHL4 of Vietnam indigenous cattle breeds using GADPH as a reference gene showed in range from 2.53 to 2.89 copies.
Parents Catechesis: for Children, Family or for Adults?
Authors: Stala Józef 
Published: September 2023

The Author of article provides an important information about parent catechesis from Catholic perspective. He reminds the basis of parent catechesis, beginning with the historical importance and explanations contained in the Church Documents: “Parents are the primary educators in the faith. Together with them, especially in certain cultures, all members of the family play an active part in the education of the younger members’ (General Directory for Catechesis255). If parents are the most important persons of the education of children in the faith, they need to be well prepared for fulfilling their mission that includes especially treating catechesis as lifelong process, the learning of Christian doctrine andmethods of family catechesis, permanent formation of mothers and fathers into the fullness of Christian life. The conclusion presents main challenges and advocates an intensification of catechetical activity in our times.
Comparison of the potential of extracts from the flower, fruit pulp, and seed of Cassia fistula L. on MCF-7 breast cancer cell growth and cell migration
Authors: BOONTHA Supavadee , BURANRAT Benjaporn , SAEPANG Kamchai , PITAKSUTEEPONG Tasana 
Published: September 2023

This study aimed to determine the anticancer effects of extracts prepared from various parts of Cassia fistula L. (CF), i.e., flower extract (FE), fruit pulp extract (FPE) and seed extract (SE), on MCF-7 breast cancer cells. The anticancer effects of the extracts were assessed for cell toxicity, cell proliferation, cell migration, cell apoptosis, and production of reactive oxygen species (ROS). Effective cancer treatments have focused on inhibiting epidermal growth factor receptor (EGFR) signalling. Thus, the expression of EGFR protein after extract-treated cells was also determined. Following a 72 incubation, high potential cytotoxicity on MCF-7 cells was observed after SE treatment, followed by FE and FPE treatment. FE, FPE, and SE significantly inhibited cell growth at concentrations of 500, 1,000, and 250 µg/mL, respectively. Also, FE, FPE, and SE markedly suppressed migration of MCF-7 cells at concentrations of 500, 500, and 100 µg/mL, respectively. These results can be concluded that SE had the highest potential anticancer effect on MCF-7 cells when compared with FE and FPE. Thus, SE might be a potential source of preventative and therapeutic agents against breast cancer. Since most anticancer drugs cause ROS production in cancer cells and it is known that ROS induce cell death; therefore, cell apoptosis and ROS formation induced by SE were further studied. The results showed SE induced MCF-7 cell apoptosis in a concentration-dependent way. SE caused a significant increase in ROS formation when compared with the control group. Western blot analysis showed low levels of EGFR protein expression after SE-treated cells at 1,000 mg/mL. Therefore, besides ROS formation, it may be concluded that the downregulation of EGFR protein expression is potentially one of the fundamental mechanisms driving the anticancer effects of SE.
Introduction pages, NBHA-CN 51(3), 2023
Authors: SESTRAS Radu E. 
Published: September 2023

The papers published in Notulae Botanicae Horti Agrobotanici Cluj-Napoca, Issue 3, Volume 51, 2023 represent new exciting research in different topics of life science, respectively in plant science, horticulture, agronomy, crop science, and forestry. Among the interesting articles we invite you to find news about: Comparison of the potential of extracts from the flower, fruit pulp, and seed of Cassia fistula L. on MCF-7 breast cancer cell growth and cell migration; Evaluation of anticancer activity of Plumbago indica root extract on cervical HeLa cancer cells; Metabolome and transcriptome analyses reveal the colouring mechanism of red honeysuckle (Lonicera japonica Thunb.); Ethylene modulates root growth and mineral nutrients levels in trifoliate orange through the auxin-signaling pathway; Green nanotechnology for plant bacterial diseases management in cereal crops: a review on metal-based nanoparticles; Physiological responses of coffee (Coffea arabica L.) plants to biochar application under water deficit conditions; Gene mining and functional analysis related to maize (Zea mays L.) seed size; Phytochemical properties, antioxidant potential and fatty acids profiling of three dragon fruit species grown under sub-tropical climate; Chemical composition and anti-inflammatory effect of Phellodendron amurense Rupr. stem bark extract; Ecotype effects on photosynthesis performance using A/PFFD among Pinus nigra Arn.; Assessment of herbaceous vegetation classification using orthophotos produced from the image acquired with unmanned aerial systems.
Psychiatric and Behavioural Disorders in Children with Epilepsy (ILAE Task Force Report): Behavioural effects of epilepsy surgery
Authors: Besag Frank , Caplan Rochelle , Aldenkamp Albert , Dunn David W. , Gobbi Giuseppe , Sillanpää Matti 
Published: September 2023

ABSTRACTThere are relatively few studies of the behavioural outcome of epilepsy surgery in children that have used standardised behavioural measures before and after the procedure. Those investigations that have used such measures are often on mixed groups with mixed pathology, implying that the numbers, when stratified, are very small. They are also often retrospective. Furthermore, because placebo surgery is generally not an option, the studies have been open and they are usually uncontrolled. The few available data suggest that, although individual children may benefit or deteriorate, there is little overall group effect of temporal or extratemporal surgery on behavioural/psychiatric outcome. Hemispherectomy has traditionally been associated with the expectation of marked behavioural improvement but firm data are lacking. Multiple subpial transection performed for electrical status epilepticus of slow‐wave sleep in the Landau‐Kleffner syndrome can result in marked improvements in cognition and behaviour. Vagus nerve stimulation appears to improve quality of life and mood/behaviour but again the quality of the data has been questioned. There is a need for large, prospective, multicentre, collaborative studies using standardised cognitive and behavioural measures before and after surgery to provide an adequate database on the outcome of various categories of procedures, pathologies and patients.
Análises Físico-Químicas dos Méis Industrializados e Artesanais Comercializados em Araguaína - TO
Authors: Carvalho Nagila Lessa de , Araújo Milena Dos Santos , Silva Sabrina Alexandre Ribeiro da , Damasceno Iangla Araújo de Melo 
Published: September 2023

O mel apresenta muitas características atrativa, e é amplamente utilizado pela população, e por isso pode ser alvo de adulterações, por inúmeros motivos, dentre eles a tentativa de aumentar seu volume, adulterando pelo acréscimo de vários tipos de açúcares de baixo custo, como açúcar de cana, açúcar refinado de beterraba, xarope de milho, teores elevados de frutose e xarope de maltose. Tendo isso em vista, o objetivo deste trabalho foi realizar uma análise das propriedades microscópicas, organoléptica e físico-químicas sendo os parâmetros estudados: reação de Fiehe, identificação de sacarose por escala Brix, acidez livre, teor de cinzas, umidade, determinação de pH, densidade, reação de lugol, para investigar a presença de adulterantes na composição do mel como também alterações decorrentes do mau acondicionamento ou processamento de seis amostras de méis, sendo três delas comercializados de forma industrial, adquiridas em supermercados e as três restantes foram obtidas em feiras livres e tem origem artesanal na cidade de Araguaína-TO. Como resultado, foi observado que as amostras apresentaram ao menos um parâmetro fora do padrão preconizado pelas legislações vigentes, conclui-se que os méis, possivelmente encontravam-se em condições inadequadas de armazenamento tendo em vista que na maioria das amostras apresentaram resultados em desacordo com os limites aceitáveis.
Palavras-chave: Mel. Hidroximetilfurfural. Físico-química. Qualidade
Abstract
Honey has many resources, and is widely used by the population, and therefore can be the target of adulteration, for several reasons, among them the attempt to increase its volume, adulterating by adding various types of low-cost sugars, such as sugar Cane, Refined Beet Sugar, Corn Syrup, High Fructose and Maltose Syrup. With this in mind, the objective of this work was to carry out an analysis of the microscopic, organoleptic and physical-chemical properties, the following requirements being observed: Fiehe reaction, identification of sucrose by Brix scale, free acidity, ash content, humidity, pH efficiency, density, Lugol reaction, to investigate the presence of adulterants in the composition of honey as well as alterations resulting from poor packaging or processing of six samples of honeys, three of which are commercialized industrially, purchased in supermarkets and the remaining three were from open markets and has artisan origin in the city of Araguaína-TO. As a result, it was observed that the samples showed at least one parameter outside the standard recommended by the current legislation, it is concluded that the honeys were possibly in established processing conditions, considering that in most samples they presented results in disagreement with the accepted limits.
Keywords: Honey, Hydroxylmethylfurfural. Physicochemical. Quality.