bluuebunny/crossref_metadata_2025_split huggingface dataset:
Integrative analysis of a Novel six methylated pseudogene Prognostic signature in patients with glioma
Authors: Chen Zhigang , Zhou Jialin , Wang Bingran , Li Jiahui , Xie Han , Zhao JiaJia , Liu Jun , Tian Dasheng , Bian Erbao 
Published: February 2023

Abstract
Background: Gliomas are the most common malignant tumor of the central nervous system(CNS). Dysregulated pseudogene expression was significantly associated with the prognosis of glioma patients. However, the role of abnormal methylation of pseudogenes in glioma prognosis has not yet been studied. This study aimed to develop a novel six-methylated pseudogenes signature to predict the prognosis of glioma patients.
Methods: Based on lasso regression analysis, a risk signature for six methylated pseudogenes was constructed. Next, a prognostic nomogram including grade, age, gender, and radiation was constructed. Besides, the immune cell infiltration analyses of patients based on the six-methylated pseudogenes were performed. Meanwhile, consensus cluster analysis of six methylated pseudogenes identified two glioma patient subgroups. Furthermore, GO, KEGG and GSEA were used to analyze related genes. Finally, the ability of glioma to proliferate, migrate and invade was used to verify subsequent functions.
Results: In this study, six gene models consisting of methylated pseudogenes were identified and validated, and showed strong prognostic power in the training dataset, validation dataset, and entire dataset. The calibration diagram showed good predictive performance. In addition, the proportion of B cells and CD4+T cells was significantly higher in the high-risk group, while the proportion of mono cells was lower. By silencing the expression of SBF1P1 and SUMO1P1, the ability of glioma to proliferate, migrate, and invade can be inhibited.
Conclusions: The six-methylated pseudogene signature may be a novel predictor for prognostic assessment of glioma patients, which could accurately predict patient prognosis.
Removal of Norfloxacin antibiotic from wastewater by adsorption onto SnS 2 followed by photocatalysis
Authors: Kar Sayani , Pal Tanusri , Ghosh Surajit 
Published: February 2023

Abstract
Antibiotic remainders found in the river water of many countries have been a significant threat to the environment and human health. Herein, the removal of Norfloxacin antibiotic from the aqueous medium has been reported. We successfully synthesized SnS2 nanoplates and studied the removal of Norfloxacin aqueous medium by adsorption followed by photocatalysis process using SnS2. The presence of pores on SnS2 surface and excellent photo-induced charge generation ability under solar light illumination makes it a potential material for the degradation of Norfloxacin from an aqueous medium. 80% of Norfloxacin was degraded in 130 minutes, out of which 36.7% was removed in 20 min by the adsorption process and the rest was degraded in 110 min photo catalytically. The scavenger experiments were also performed to determine the reactive species responsible for the photodegradation. The results identified that the holes play a dominating role in the photodegradation of Norfloxacin by SnS2 in the aqueous medium. Our study has established SnS2 as a promising material for the removal of water pollutants, which may be a pathway to protect the environment from antibiotic pollution in river water.
Fluorescent Aptasensor Based on Fe3O4@PPy
Authors: Jiao Zhe , Jiao Shaohe , Wang Yueting , Chen Weibin , Xie Yangzi , Zhang Xiaolin , Yi Long 
Published: February 2023

Abstract
In this work, nanomaterial polypyrrole-coated magnetite nanoparticles (Fe3O4@PPy) was synthesized and combined with label free nucleic acid probe for aptasensing. Two kinds of nucleic acid probes were applied, including blue emissive 1,1,2,2-tetrakis [4-(2-bromo-ethoxy) phenyl] ethene (TTAPE) and red emissive malachite green. The two probes can be interacted with the aptamer to form aptasensor by label free strategy. Fe3O4@PPy not only adsorbed aptamer by electrostatic interaction as well as the π–π bonding and hydrogen bonding (between PPy coating and the phosphate backbone of DNA), but also quenched the fluorescence of fluorescent probes. Upon addition of target compounds, the aptasensor was away from Fe3O4@PPy and magnetic separated thus lower background noise was obtained. The turned-on photoluminescence (PL) emission intensity was recorded and showed linearity to the concentrations of targets. The aptasensor was applied to detect metal ions, small molecular acetamiprid, and thrombin protein by changing different aptamers. The robustness of method was proved by application in real samples including vegetables (for detection of acetamiprid, LODs 0.02 and 0.04 ng/L), serum samples (for detection of thrombin, LODs 5.5 and 4.3 nM) and water samples (for detection of Pb, LOD 0.17 nM). The recoveries ranged from 81 to 105%, with RSDs ranged from 4.3 to 9.2%. Given its high selectivity and sensitivity, this technique can be used as a general detection platform for clinical and environmental applications.
A comparison study of ancient and modern wheat: Characterization and sensory analysis of an ancient Anatolian wheat Triticum dicoccum (Kavilca) grown in Kars, Türkiye
Authors: Aydar Elif Feyza , Demircan Evren , Mertdinç Zehra , Kahraman Tülay , Yaman Hümeyra , Salantur Ayten , Çetiner Buket , Özçelik Beraat 
Published: February 2023

Abstract
The ancient wheat varieties have been ignored due to efforts to increase the yield of modern wheat. In recent years, interest in ancient wheat has increased for various reasons. This study aimed to characterize the ancient Anatolian wheat Triticum turgidum ssp. dicoccum (Kavilca) from Kars, Türkiye and to compare it with modern bread wheat Triticum aestivum in terms of quality parameters, mineral and fatty acid profile, total flavonoid content (TFC), and antioxidant activity (TAA). Quality parameters were conducted with standardized methods. Mineral and fatty acid profiles were determined with inductively coupled plasma mass spectrometry and gas chromatography, respectively. Spectrophotometric methods were used to determine TFC and TAA. Triangular test and Quantitative Descriptive Profile method were applied for sensory analyses. Novel findings of this study were low gluten quality (p < 0.05), low Al content (2.85–5.16 mg/kg dw, p < 0.05), and high TFC (3.65 mg RE/g dw, p < 0.05) of Kavilca along with similar overall liking sensory score (p > 0.05) compared to modern bread. There was a strong sensory difference with a 99.9% probability between modern and 15% Kavilca added bread. Characterization and especially, sensory analysis results might be a reference for the food industry to integrate Kavilca into several bakery products.
Effect of P-glycoprotein Inhibition on Temozolomide Chemosensitivity on the Neuroblastoma Cell Line Sh-sy5y
Authors: KARADEMİR Mustafa 
Published: February 2023

Abstract
Neuroblastoma is one of the most common and deadly childhood solid tumors. P-glycoprotein (P-gp) pump plays a role in developing resistance to many chemotherapeutic agents. The high expression of P-gp is associated with poor prognosis in drug resistance and neuroblastoma treatment. We aimed to evaluate the anticancer effect of Temozolomide (TMZ) on the SH-SY5Y human neuroblastoma cell line in the presence of P-gp inhibitor Verapamil (VER). In the present study, the antiproliferative effect of TMZ on SH-SY5Y cells alone and in combination with VER was evaluated using a colorimetric XTT viability test. SH-SY5Y cells were seeded to 96 well plates at 10,000 cells/well. TMZ (100 µM-10 mM) and VER (0.25-10 µg/ml) were applied to the cells alone first, then XTT measurements were performed after 24 and 48 hours. In the study's second phase, VER was applied to the cells at the fixed concentration of 2.5 µg/ml to block P-gp pumps, then increasing concentrations of TMZ were applied to the cells in the presence of VER. The current study showed that in addition to cytotoxic effects, VER + TMZ administration was found to accelerate apoptosis in SH-SY5Y cells compared to TMZ utilization alone. The wound healing assay demonstrated that VER + TMZ combination also inhibited cell migration. In light of this evidence, combining TMZ with VER in the neuroblastoma cell line may inhibit migration and cell proliferation via the apoptosis pathway and produce a substantial anticancer effect.
Effect of copper azole preservative on the laminate’s surface wettability and interlaminar shear performance of preservative treated Glulam
Authors: Yang Xiaojun , Liu Jiamin , Wang Chaojie , Kong Xiaohui 
Published: February 2023

Abstract
Copper azole (CA) preservative applied to glulam improves the durability of glulam. The purpose of this study is to evaluate the effect of CA preservative on the surface bonding quality of laminates under different surface conditions. Surface morphology and wettability of CA preservative treated laminate were investigated. A comparison was also made of interlaminar shear performance obtained from preservative treated glulam under different environmental conditions. The results indicate that CA preservative adheres to wood fibers and occupies part of wood voids, which has a significant adverse effect on water permeability of preservative treated laminate. Planing can effectively improve the surface wettability of preservative treated laminate. CA preservative tiny particles on the planed laminate surface are distributed relatively uniformly, and the proportion is smaller than that of the unplaned laminate. The interlaminar shear strength of preservative treated glulam made of planed laminates is at least 15% higher than that of the glulam made of unplaned laminates. No matter in hot and humidity environment or natural aging tests, preservative treated glulam glued by resorcinol formaldehyde (RF) adhesive performed better than that by polyurethane (PUR) adhesive. It is recommended that in order to obtain a reliable preservative treated glulam quality, the 0.6mm planing of laminate and RF adhesive are required.
Development of Disaster Response Strategies for Multi-Disasters
Authors: Tsai Meng-Han , Chan Yi-Lin 
Published: February 2023

Abstract
The purpose of this research is to develop a disaster response strategy that can be used when more than one disaster happens at the same time. When different types of disasters occur at the same time, disaster response operations will become extremely complex. It is difficult for responders to directly perform response operations based on a single standard operation procedure. This research used flooding events that occurred in Taiwan during the COVID pandemic as an example case to develop the response strategies. The standard operating procedures before and after the pandemic were first reviewed. The authors also joined the response operations to have close observations on how responders execute response operations under restrictions of the pandemic. User interviews were then conducted to collect feedback from four responders. Finally, the multi-disaster response strategies, coined as OPERATE, was developed based on results of procedure review, field observations, and user interviews. OPERATE was developed for disaster response teams to smoothly execute response operations when multiple disasters occur simultaneously. It includes seven perspectives: simplified operation, flexible procedures, adjustable environment, personalized reminder, positive attitude, well-developed decision support tool, and preparatory education. OPERATE is developed to reduce the impact of multi-disasters on response teams, including the impact of the disaster itself and orders issued by other government departments in response to other disasters. Following the developed strategies, disaster response teams are expected to conduct stable and efficient operations in the context of multiple disasters.
Comprehensive analysis of prognostic value and immune infiltration of CDK1 in lung cancer
Authors: Zhu Yunying , Yu Qinhua , Lei Chenshuang , Qiu Liannv 
Published: February 2023

Abstract
Objective:
Lung cancer is one of the common malignant tumors worldwide with poor prognosis. Cyclin dependent kinase 1 (CDK1) plays crucial roles in regulating of cell cycle transition and is mainly involved in the development and progression of various tumors, however, little is known about its function in lung cancer.
Methods:
The Cancer Genome Atlas (TCGA), Gene Expression Profiling Interactive Analysis (GEPIA), Clinical Proteomic Tumor Analysis Consortium (CPTAC) and Human Protein Atls (HPA) were used to evaluate the level of CDK1. Kaplan-Meier (K-M) Plotter, receiver operating characteristic (ROC) curve and Cox regression analyses were used to evaluate the value of CDK1 in lung cancer. Gene functional and pathways were assessed using Gene ontology (GO) analysis and gene set enrichment analysis (GSEA) The immune infiltration analysis was investigated basing on Single-sample gene set enrichment analysis (ssGSEA) .
Results:
The expression of CDK1 was significantly upregulatedin lung cancer patients, especially in those with advanced stage. Elevated CDK1 was associated with advanced clinical stage, shorter overall survival (OS) and disease specific survival (DSS) in lung cancer. Moreover, the functions of CDK1 were primarily related to cell cycle regulation and immune response. Most interestingly, the expression of CDK1 was associated with the infiltration immune cells and immune checkpoints.
Conclusions:
Our results revealed that CDK1 may be a diagnostic and prognostic biomarker in lung cancer. CDK1 plays a role in tumor immune evasion and may provide a potential therapeutic target to overcome immunity resistance in lung cancer.
Examination of alternative eGFR definitions on the performance of deep learning models for detection of chronic kidney disease from fundus photographs
Authors: An Songyang , Vaghefi Ehsan , Yang Song , Xie Li , Squirrell David 
Published: February 2023

AbstractDeep learning (DL) models have shown promise in detecting chronic kidney disease (CKD) from fundus photographs. However, previous studies have utilized a serum creatinine-only estimated glomerular rate (eGFR) equation to determine CKD despite the existence of more accurate methods. In this study, we used the UK Biobank as a test and validation dataset to demonstrate an incremental and statistically significant improvement in model performance for predicting CKD when using a creatinine and cystatin C eGFR equation over a creatinine-only equation. Attempts to directly compare our results with the results from existing DL models is complicated due to significant differences in the composition of the dataset, particularly in the incidence rate of confounding risk factors. We hypothesize that existing eGFR equations' limitations in accurately identifying CKD and the paucity of retinal features uniquely indicative of CKD may contribute to the observed differences in model performance, highlighting the need for more principled research to quantify the effects of dataset distribution on DL models' ability to predict CKD from fundus photographs.
Primary Dural Lymphoma Case Report: A Distinct Subtype of Primary Central Nervous System Lymphoma
Authors: Sushanth Sreenivasan , Risha Solanki , Kancharla Pragnan , Khan Cyrus , Samhouri Yazan 
Published: February 2023

Abstract
Primary CNS lymphoma (PCNSL) is an aggressive form of extranodal non-Hodgkin lymphoma that arises in the brain parenchyma, eyes, meninges, or spinal cord in the absence of systemic disease. Primary dural lymphoma (PDL) arises from the dura mater of the brain. It differs biologically from other CNS lymphomas. It is usually a low-grade B-cell marginal zone lymphoma (MZL), whereas other types of PCNSL are usually high-grade large B-cell lymphoma. This specific pathological subtype has important therapeutic and prognostic implications, making PDL a distinct subtype of PCNSL. Herein, we report a case of PDL in an African American patient who presented with chronic headaches. MRI of the brain showed a dural-based homogeneously enhancing extra-axial mass along the left hemisphere and was contained within the anterior and parietal dural matter. The malignant cells stained diffusely positive for CD19, CD20, and CD22 but negative for CD5, CD10, Cyclin D1, and CD56. Ki67 was 10–20%, consistent with extranodal marginal zone lymphoma. Given the location and pathology, the patient was diagnosed with PDL. She was started on treatment with bendamustine and rituximab. Due to Marginal Zone Lymphoma’s indolent nature, location outside the blood brain barrier, and known efficacy of bendamustine-rituximab, we decided to treat our patient with Bendamustine-Rituxumab. She completed six cycles, and post-therapy MRI brain showed complete remission. Our case adds to the sparse literature about primary dural lymphoma.
Osmotic regulation of free amino acid for adaptation to high salt in razor clam
Authors: Deng Min , Wang Honglei , Du Xinxin , Yuan Li , Li Yifeng , Niu Donghong 
Published: February 2023

Abstract
Free amino acids play an important role in osmotic regulation induced by high salt. In order to study the effect of free amino acids on the osmotic regulation of Sinonvacula constricta cells, the salinity was set at 20 and 32 in control group and experimental group. In the experimental group, proline, taurine and alanine amino acid solid powder with concentration of 5ug/ml was added. Serum and cell samples were taken from each group at different time points for detection by osmotic pressure and detection by flow cytometry. In summary, we found that serum osmolality, total free amino acid and single free amino acid content increased with the increase of salt stress, and remained at a high level. Under high salt conditions, the osmotic pressure in S. constricta could not equilibrate with the seawater environment. In the later stages of stress, the gap between the two gradually widened. Under the condition of amino acid addition, the osmotic pressure is gradually the same as that of foreign seawater. Furthermore, the mean number of fluorescent channels produced by high salt downflow cytometry was significantly lower than that of the other amino acid groups. Among the three amino acids, proline has the largest accumulation in the S. constrictaand the shortest regulation time for osmotic balance. It has been shown that free amino acids can help better osmotic regulation of hypersaline S. constricta. The results of this study provide a reference for exploring the physiological mechanism of the amino acid on salt tolerance in bivalves.
Agent-based management and coordination of aircraft at intersections
Authors: OUASSAM ELHOUCINE , DABACHINE YASSINE , HMINA NABIL , BOUIKHALENE BELAID 
Published: February 2023

Abstract
This work presents a game-theoretic approach to address the problem of coordination and scheduling of aircraft at intersections, with the goal of avoiding conflicts and potential collisions. The proposed algorithm enables simple agents to work together in a way that leads to cooperative behaviors, resulting in equilibria that improve the overall efficiency of the system. The researchers tested and compared the game-theoretic approach with a centralized approach, specifically First-Come-First-Serve (FCFS), using data from Mohammed 5 Casablanca airport. The initial results suggest that the game-theoretic model is promising, despite its higher complexity. The approach has the potential to improve the overall coordination and scheduling of aircraft, leading to a more efficient and safe system. The proposed game-theoretic approach is designed to improve the coordination and scheduling of aircraft at intersections, ultimately leading to a safer and more efficient system. The approach is shown to be promising in initial testing, offering a potentially superior alternative to centralized approaches like FCFS. This research highlights the potential benefits of game-theoretic models in addressing complex coordination problems in multi-agent systems.
Molecular mechanism of CCDC106 regulating the p53-Mdm2/MdmX signal axis
Authors: Zhou Ting , Cheng Xiyao , Ke Zhiqiang , Ma Qianqian , Xiang Jiani , Gao Meng , Huang Yongqi , Su Zhengding 
Published: February 2023

Abstract
The tumor suppressor p53 (p53) is regulated by murine double minute 2 (Mdm2) and its homologous MdmX in maintaining the p53 basal level. The overexpressed Mdm2/MdmX inhibit the cellular p53 activity, highly relevant to cancer occurrence. The coiled-coil domain-containing protein 106 (CCDC106) has been identified as a p53-interacting partner. However, its molecular mechanism is still elusive. Here we show that CCDC106 functions as a signaling regulator of the p53-Mdm2/MdmX axis mediated by cellular p53. We identified that CCDC106 directly interacts with the p53 transactivation domain by competing with Mdm2 and MdmX. The CCDC106 overexpression downregulates the cellular level of p53 and Mdm2/MdmX, and the decreased p53 reversibly downregulates the cellular level of CCDC106. Our work not only provides a molecular mechanism of CCDC106 regulating the cellular levels of p53 and Mdm2/MdmX, but also suggests that the CCDC106-p53 interaction as a novel target for cancer therapy.
Potential of GIS and remote sensing in mapping land degradation: catchment of the Manyame River, Zimbabwe
Authors: Muhoyi Hardlife , Muhoyi Edgar 
Published: February 2023

Abstract
The Manyame River Catchment area in Zimbabwe, is experiencing severe land degradation mainly due to legal and illegal land husbandry practices. These practices are negatively impacting on the sustainability of the existing ecosystems. The conditions of land can be inferred using its vegetative cover e.g., Normalised Difference Vegetation Index (NDVI). Quantitative data relating to land degradation based on notable physical features such as gullies, for Manyame River Catchment at landscape scales is poor. This study focused on the distribution and magnitude of land degradation in the Manyame River Catchment area. The study mapped out the contours of human induced land degradation using a residual trend analysis (RESTREND) method. In particular, the study used remote sensed data (NDVI and precipitation time series) to analyse the shifts over period 2000 to 2017. The analysis used R statistical software packages (RESTREND and Kendal) and Geographic Information System (GIS) techniques to quantify the degradation trends. The results indicated extracts of those areas which experienced significant human induced land degradation during the study period. RESTREND effectiveness was assessed using Mann Kendal Test. The results of this study can be used by natural resources practitioners in monitoring, assessing and managing environmental changes using GIS tools.