bluuebunny/crossref_metadata_2025_split huggingface dataset:
An Updated Surgical Approach of Using Flap and Cutaneous Nerve in the Lateral Arm to Reconstruct Complex Injuries of the Forearm and Hand
Authors: Xu Heng , Guo Linxiumei , Wang Xin , Pan Jiadong 
Published: August 2023

Background
Complex injuries involving the nerves and other soft tissues in the forearm and hand lead to functional and aesthetic defects. In such situations, multiple types of nerve autografts and flap donor sites are available. However, multiple donor sites cause donor morbidity in different locations and may lead to awkward operational positions. Therefore, based on the anatomical characterization, we aimed to modify the utilization of the lateral arm donor site for reconstruction, which restricts donor morbidity in the affected upper extremity.

Methods
We report a case series (N = 6) using a lateral arm flap (LAF) to reconstruct complex soft tissue defects in the forearm, palm, and finger. The posterior antebrachial cutaneous nerve (PACN) is the primary option for nerve bridging, whereas the LAF can carry the lower lateral brachial cutaneous nerve (LBCN) as a sensory flap. Once the PACN was insufficient, the LBCN was harvested simultaneously. All the cases included in this study were performed between January 2012 and August 2021. Demographic information, flap and nerve characteristics, complications, and hand function were analyzed.

Results
The LAF plus PACN or plus LBCN as nerve autograft, both successfully repaired 6 complex injuries: 2 cases in the forearm side, 1 in the hand palm, and 3 in the finger defects. Posterior antebrachial cutaneous nerve was the most used (8–15 cm), and LBCN plus PACN was used to bridge nerve defects when necessary (in total, 20 and 21 cm). The average follow-up time was 19.7 months. The disabilities of the arm, shoulder and hand score ranged between 6 and 12, and the mean 2-point discrimination values ranged between 6 and 12. The Semmes-Weinstein monofilament test result was under 5.46. In addition, 2 patients underwent a secondary debulking surgery. The average length of hospital stay was 10.4 days. Hematoma occurred in 2 cases, and all patients reported numbness in the donor nerve innervated areas.

Conclusions
This surgical refinement can reconstruct complex injuries in the forearm and hand. In addition, this approach restricts donor morbidity in the affected limb, comforts the operational position, and is achieved under brachial plexus anesthesia.
Understanding the Role of Adipocytes and Fibroblasts in Cancer
Authors: Downer Mauricio A. , Griffin Michelle F. , Morgan Annah G. , Parker Jennifer B. , Li Dayan J. , Berry Charlotte E. , Liang Norah E. , Kameni Lionel , Cotterell Asha C. , Akras Deena , Valencia Caleb , Longaker Michael T. , Wan Derrick C. 
Published: August 2023

Abstract
Cancer is currently the second leading cause of death in the United States. There is increasing evidence that the tumor microenvironment (TME) is pivotal for tumorigenesis and metastasis. Recently, adipocytes and cancer-associated fibroblasts (CAFs) in the TME have been shown to play a major role in tumorigenesis of different cancers, specifically melanoma. Animal studies have shown that CAFs and adipocytes within the TME help tumors evade the immune system, for example, by releasing chemokines to blunt the effectiveness of the host defense. Although studies have identified that adipocytes and CAFs play a role in tumorigenesis, adipocyte transition to fibroblast within the TME is fairly unknown. This review intends to elucidate the potential that adipocytes may have to transition to fibroblasts and, as part of the TME, a critical role that CAFs may play in affecting the growth and invasion of tumor cells. Future studies that illuminate the function of adipocytes and CAFs in the TME may pave way for new antitumor therapies.
Vascularized Composite Allotransplantation of the Hand
Authors: Laspro Matteo , Onuh Ogechukwu C. , Carrion Kassandra , Brydges Hilliard T. , Tran David L. , Chaya Bachar F. , Parker Augustus , Thanik Vishal D. , Sharma Sheel , Rodriguez Eduardo D. 
Published: August 2023

Background
Hand transplantation (HT) has emerged as an intervention of last resort for those who endured amputation or irreparable loss of upper extremity function. However, because of the considerable effort required for allograft management and the risks of lifelong immunosuppression, patient eligibility is critical to treatment success. Thus, the objective of this article is to investigate the reported eligibility criteria of HT centers globally.

Methods
A systematic review of the HT literature was conducted according to Preferred Reporting Items for Systematic Review and Meta-Analyses guidelines, using PubMed, Cochrane, Ovid/Medline, and Scopus. Program Web sites and clinicaltrials.gov entries were included where available.

Results
A total of 354 articles were reviewed, 101 of which met inclusion criteria. Furthermore, 10 patient-facing Web sites and 11 clinical trials were included. The most reported criteria related to the capacity to manage the allograft posttransplantation, including access to follow-up, insurance coverage, psychological stability, and history of medical compliance. Other factors related to the impact of immunosuppression, such as active pregnancy and patient immune status, were less emphasized.

Conclusions
Because of the novelty of the field, eligibility criteria continue to evolve. While there is consensus on certain eligibility factors, other criteria diverge between programs, and very few factors were considered absolute contraindications. As the popularity of the field continues to grow, we encourage the development of consensus evidence-based eligibility criteria.
Bioinformatics applications under condition control: high diagnostic value of DDX47 in real medical settings
Authors: Zhu Yukun , Cai Wei , Zheng Ying , Zhang Wei , Wang Bo , Kang Yan 
Published: August 2023

Abstract
Sepsis is an organ dysfunction caused by a dysregulated host response to infection and remains an ongoing threat to human health worldwide. Septic shock is the most severe subset of sepsis as characterized by abnormalities in cells, circulation and metabolism. As a time-dependent condition, early recognition allowing appropriate therapeutic measures to be started in a timely manner become the most effective way to improve prognosis. However, due to the lack of a gold standard, most diagnoses merely rely on medical history, empirical diagnosis and blood culture results. Gene expression profiles have specific diagnostic value, as they reflect a subjective host response to pathogens. We propose a method, Condition Control based on Real-life Medical Scenarios, to control for factors in realistic medical scenarios. Restricted variables are used as much as possible to identify unique differential genes and progressively test their diagnostic value by relaxing restrictions. In total, three datasets were included in the study; the first two datasets were from the Gene Expression Omnibus (GEO) database, and the third involved patients who were diagnosed with sepsis or septic shock within 7 days of admission to the ICU at West China Hospital of Sichuan University from 2020 to 2021. DDX47 showed preferable diagnostic value in various scenarios, especially in patients with common infections or sepsis and septic shock. Here we also show that hub genes may regulate immune function and immune cell counts through the interaction of different apoptotic pathways and immune checkpoints based on the high correlation. DDX47 is closely associated with B cells according to single-cell sequencing results.
Multi-scale spatial mapping of cell populations across anatomical sites in healthy human skin and basal cell carcinoma
Authors: Ganier Clarisse , Mazin Pavel , Herrera-Oropeza Gabriel , Du-Harpur Xinyi , Blakeley Matthew , Gabriel Jeyrroy , Predeus Alexander , Cakir Batuhan , Prete Martin , Harun Nasrat , Darrigrand Jean-Francois , Haiser Alexander , Wyles Saranya , Shaw Tanya , Teichmann Sarah A. , Haniffa Muzlifah , Watt Fiona M. , Lynch Magnus D. 
Published: August 2023

AbstractOur understanding of how human skin cells differ according to anatomical site and tumour formation is limited. To address this we have created a multi-scale spatial atlas of healthy skin and basal cell carcinoma (BCC), incorporatingin vivooptical coherence tomography, single cell RNA sequencing, spatial global transcriptional profiling andin situsequencing. Computational spatial deconvolution and projection revealed the localisation of distinct cell populations to specific tissue contexts. Although cell populations were conserved between healthy anatomical sites and in BCC, mesenchymal cell populations including fibroblasts and pericytes retained signatures of developmental origin. Spatial profiling andin silicolineage tracing support a hair follicle origin for BCC and demonstrate that cancer-associated fibroblasts are an expansion of aPOSTN+ subpopulation associated with hair follicles in healthy skin.RGS5+pericytes are also expanded in BCC suggesting a role in vascular remodelling. We propose that the identity of mesenchymal cell populations is regulated by signals emanating from adjacent structures and that these signals are repurposed to promote the expansion of skin cancer stroma. The resource we have created is publicly available in an interactive format for the research community.Significance statementSingle cells RNA sequencing has revolutionised cell biology, enabling high resolution analysis of cell types and states within human tissues. Here, we report a comprehensive spatial atlas of adult human skin across different anatomical sites and basal cell carcinoma (BCC) - the most common form of skin cancer - encompassingin vivooptical coherence tomography, single cell RNA sequencing, global spatial transcriptomic profiling and in situ sequencing. In combination these modalities have allowed us to assemble a comprehensive nuclear-resolution atlas of cellular identity in health and disease.
Defined human PSC culture conditions robustly maintain human PSC pluripotency through Ca2+signaling
Authors: Eidhof Ilse , Kele Malin , Shasavani Mansoureh , Ulfenborg Benjamin , Winn Dania , Uhlén Per , Falk Anna 
Published: August 2023

SummaryHuman pluripotent stem cells (hPSCs) have significant potential for disease modeling and cell therapies. However, their clinical applicability is limited due to the need for undefined conditions for PSC cultivation, which increase the risk of immunogenicity, result in batch-to-batch variability and finite scalability. These limitations may be circumvented by xeno-free, defined culture conditions. However, biological processes that preserve robust, homogenous PSCs in defined conditions remain to be characterized. Here, we compared gene expression data from over 100 hPSC cell lines cultivated in undefined and defined culture conditions. Defined culture conditions significantly reduced inter-PSC line variability, highlighting the importance of standardization to minimize PSC biases. This variability is concurrent with decreased germ layer differentiation and increased expression of Ca2+-binding proteins. The significance of tightly controlled Ca2+signaling in hPSC pluripotency in defined culture conditions was also confirmed. A deeper understanding of these processes may aid in standardizing defined hPSC culture conditions.Graphical abstractIn BriefEidhof et al. compared gene expression array data obtained from more than 100 hPSC cell lines to study the biological differences between PSCs cultivated under defined and undefined culture conditions.HighlightsDefined culture conditions reduce inter-hPSC line variability.Defined conditions decrease the expression of germ layer differentiation markers.Ca2+signaling associated genes capture specific hPSC states in defined conditions.High Ca2+activity drives pluripotency, low Ca2+activity drives differentiation.
Spatially distinct inputs modulate the amount of active Mitotic-phase GAP to locally restrict RhoA signaling for successful cell division
Authors: Wolff Friederike , Srinivasan Swathi , Nöcker Marie Christin , Mangal Sriyash , Mikeladze-Dvali Tamara , Zanin Esther 
Published: August 2023

AbstractAt the end of mitosis a contractile ring consisting of filamentous actin (F-actin) assembles at the cell equator and ring constriction equally partitions the cellular content. Inhibitory and stimulatory signaling cascades spatially limit RhoA activity to a narrow central zone to promote F-actin polymerization only at the cell equator. While the stimulatory signal is well-characterized, the mechanisms by which inhibitory signals restrict active RhoA to a narrow equatorial zone and prevent improper RhoA activity at the cell poles are not known. Here, we identify two regulatory inputs that control the activation and cortical targeting of the Mitotic-phase GTPase activating protein (MP-GAP) for RhoA which limits RhoA activity both at the cell poles and at the cell equator. We show that at cell poles, MP-GAP is a direct phosphorylation target of spindle-pole associated Aurora A kinase. We identify three Aurora A target residues in a region of MP-GAP that binds directly to its catalytic GAP domain suggesting the Aurora A phosphorylation releases MP-GAP autoinhibition. We show that phosphorylation of these sites is required to keep RhoA activity low at the cell poles. At the cell equator, F-actin facilitates MP-GAP enrichment, which opposes RhoA GEF activity and thereby accelerates RhoA flux through the GTPase cycle for the formation of a narrow equatorial zone of active RhoA. Thus, the amount of active MP-GAP is modulated by two distinct regulatory inputs that function in spatially restricted locations: Aurora A phosphorylation relieves MP-GAP autoinhibition to limit RhoA activity at the cell poles, while F-actin polymerization promotes MP-GAP targeting to prevent distribution of active RhoA in a broad zone at the cell equator. By determining the mechanism of spatially confining RhoA activity at the equator and the cell poles during cytokinesis, our work has broad implications to how Rho activity zones are formed and maintained during cytokinesis and how defects in their formation impact animal development and disease.
Effect of Ti on stress corrosion cracking behavior and mechanism of Monel K500 alloy in flowing seawater
Authors: Xi Yuchen , Wang Qinying , Luo Xiaofang , Zhang Xingshou , Liu Tingyao , Zheng Huaibei , Dong Lijin , Wang Jie , Zhang Jin 
Published: August 2023

Purpose
The purpose of this paper is to investigate the effect Ti on stress corrosion cracking (SCC) and flow accelerated stress corrosion cracking (FA-SCC) behavior and mechanisms of Monel K500 alloy.

Design/methodology/approach
Monel K500 alloy with different Ti contents was designed. A metallurgical microscope (XJP-3C) and scanning electron microscopy (EV0 MA15 Zeiss) with an energy dispersive spectroscopy were used to analyze the microstructure of the Monel K500 alloy. In situ electrochemical tests were carried out in static and flowing seawater to study FA-SCC behavior.

Findings
The number of TiCN particles in the alloy increased as the increase of Ti content. The static corrosion and SCC of Monel K500 alloy are reduced as the content of Ti increases. Generally, the SCC of alloys was caused by the synergistic effect of the anodic dissolution at exposed metal matrix and the pit corrosion of metal matrix adjacent to TiCN particles, which was further accelerated by flowing.

Originality/value
The corrosion behavior and mechanism of Monel K500 alloy with different Ti contents in a complex flowing seawater environment are still unclear, which remain systematic study to insure the safe service of the alloy.
Navigating the crowdfunding landscape: a study of knowledge trajectories based on main path analysis
Authors: Rejeb Abderahman , Rejeb Karim , Appolloni Andrea , Treiblmaier Horst 
Published: August 2023

PurposeCrowdfunding (CF) has become an increasingly popular means of financing for entrepreneurs and has attracted significant attention from both researchers and practitioners in recent years. The purpose of this study is to investigate the core content and knowledge diffusion paths in the CF field. Specifically, we aim to identify the main topics and themes that have emerged in this field and to trace the evolution of CF knowledge over time.Design/methodology/approachThis study employs co-word clustering and main path analysis (MPA) to examine the historical development of CF research based on 1,528 journal articles retrieved from the Web of Science Core Collection database.FindingsThe results of the analysis reveal that CF research focuses on seven themes: sustainability, entrepreneurial finance, entrepreneurship, fintech, social entrepreneurship, social capital, and microcredits. The analysis of the four main paths reveals that equity CF has been the dominant topic in the past years. Recently, CF research has tended to focus on topics such as fintech, the COVID-19 pandemic, competition, Brexit, and policy response.Originality/valueTo the authors' best knowledge, this is the first attempt to explore knowledge diffusion dynamics in the CF field. Overall, the study offers a structure for analyzing the paths through which knowledge is diffused, enabling scholars to effectively manage a large volume of research papers and gain a deeper understanding of the historical, current, and future trends in the development of CF.
How does corporate social responsibility contribute to innovation performance? The moderating role of social media strategic capability and big data analytics capability
Authors: Wang Chenxiao , Tang Fangcheng , Zhang Qingpu , Zhang Wei 
Published: August 2023

PurposeThe purpose of this study is to investigate the impact of corporate social responsibility (CSR) on innovation performance and examine the moderating role of social media strategic capability and big data analytics capability. Specifically, the authors explore the effects of both external and internal CSR on innovation performance.Design/methodology/approachThe authors collected data from 221 senior, middle and research and development (R&D) managers of high-tech firms in China, using a questionnaire survey with a six-month interval.FindingsThe empirical results show that both external and internal CSR positively influence innovation performance. Furthermore, social media strategic capability has a positive moderating effect on the relationship between CSR and innovation performance, while big data analytics capability moderates the relationship between external CSR and innovation performance.Research limitations/implicationsThe data comes from high-tech firms in China, which may limit the generalizability and external validity of the findings. Future studies should replicate this study in other industries and types of organizations.Practical implicationsThe study suggests that high-tech firms should engage in both external and internal CSR activities to promote innovation performance. Moreover, leveraging social media strategic capability and big data analytics capability can enhance innovation performance.Originality/valueThis study contributes to the literature on CSR outcomes by empirically exploring the effects of external and internal CSR on innovation performance, thus extending stakeholder theory. Additionally, by revealing the contingency effects of social media strategic capability and big data analytics capability, this study enriching the research on dynamic capabilities theory in the context of digital transformation.
Understanding university enterprise collaboration for disaster resilience in South-East Asia
Authors: Tucker Rebeka Catherine , Liyanage Champika , Robinson Sarita Jane , Montebon Darryl Roy , Gotangco Gonzales Charlotte Kendra , Olpoc Joselito C. , Patacsil Liza B. , Tantanee Sarintip , Buranajarukorn Panu , Apichayaku Orawan Sirisawat , Weerasinghe Rukmal N. , Dissanayake Rsanjith 
Published: August 2023

Purpose
This paper is part of the ERASMUS+-funded Strengthening University Enterprise Collaboration for Resilient Communities in Asia (SECRA) project. This study aims to map collaborative architecture between partner universities and the public/private sectors to provide a contextualised collaboration framework for disaster resilience (DR) in South-East Asia.

Design/methodology/approach
Documentary reviews were conducted in partner countries to establish the current context of university enterprise collaborations (UEC) in South-East Asia. A concept-centric approach permitted the synthesis of concepts from each country review, allowing for comparisons between collaborative practices that impact the success of DR collaborations.

Findings
The review identified that funding, continuity, long-term strategic plans and practical implementation are lacking in partner countries. However, each country demonstrated good practices and identified enablers and barriers that impact DR collaborations.

Research limitations/implications
The synthesis revealed a lack of a practical understanding of real-world barriers. Further research is needed to understand real-world experiences in DR collaborations and to provide insights into barriers, enablers and good practices in DR collaborations. Gaining an “on-the-ground” perspective will provide detailed insights and the feasibility of implementation.

Practical implications
The findings provide the foundations for developing a heuristic UEC framework that can inform policies and practices for DR in partner countries.

Social implications
The findings can inform various stakeholder policies and practices and promote the exchange of ideas between stakeholders to enhance DR in South-East Asia.

Originality/value
The results are relevant within the South-East Asian, as governments have intensified the adoption of measures to encourage UEC for DR.
Evaluation of animal safe places for emergency evacuation in the Hunter Region of New South Wales, Australia
Authors: Ogunmakinde Olabode Emmanuel , Egbelakin Temitope , Henderson Robert 
Published: August 2023

Purpose
This project aims to improve understanding of disaster preparedness and resilience, as well as animal recovery during a disaster event. The purpose of this study is to determine the type and extent of repair/renovation work required to bring proposed animal safe places and their different components up to the minimum standard for an operational animal evacuation site.

Design/methodology/approach
On-site survey using a five-point Likert scale questionnaire was used to collect data through a five stage processes beginning with the creation of a facility register and continuing with the development of assessment guidelines, assessment planning, actual assessment and reporting. In the Hunter Region of New South Wales, Australia, 25 potential animal evacuation sites, including saleyards, showgrounds, animal shelters and racecourses, were identified and accessed.

Findings
The findings of the study reveals that none of the facilities are of high standard. However, 16% would require cosmetic work, 76% would require minor work and the remaining facilities would require significant work. According to the characteristics of the facilities assessed, saleyards have a greater capacity to accommodate animals ranging in size from large to small. More than half of the facilities have average to excellent hay sheds and wash bays, whereas less than half have dedicated animal quarantine space.

Research limitations/implications
The findings of this study should assist government agencies, local councils, emergency management teams and other stakeholders in planning for and considering renovation/upgrade of potential animal safe places.

Originality/value
The assessment guideline developed as part of this research can be included in the local council’s emergency management plan to improve adequate planning for safe animal evacuation.