bluuebunny/crossref_metadata_2025_split huggingface dataset:
Clinical Impact of Antipolyethylene Glycol (PEG) Antibody in Hematological Patients Administered PEGylated‐Granulocyte Colony‐Stimulating Factor
Authors: Okada Naoto , Taro Shimizu , Ando Hidenori , Nakamura Shingen , Goda Mitsuhiro , Abe Masahiro , Kitahara Takashi , Ishida Tatsuhiro , Ishizawa Keisuke 
Published: January 2023

AbstractPolyethylene glycol (PEG) is a polymer covalently attached to proteins to improve their half‐life and efficacy. We previously reported that the PEGylated granulocyte colony‐stimulating factor (PEG‐G‐CSF) is immunogenic, which could adversely impact drug efficacy and safety in animal models. Here, we analyzed the relationship between anti‐PEG antibody titers and the clinical impact of PEG‐G‐CSF in 19 hematological patients. A gradual decrease of anti‐PEG antibody titers from baseline was observed after PEG‐G‐CSF administration. Of the 19 participants, 10 were assessed for noninfectious fever after the first administration of PEG‐G‐CSF and three experienced this reaction. The receiver operating characteristic curve revealed that the cut‐off values of pretreated anti‐PEG IgM and IgG titers for noninfectious fever were set at 5.0 and 96.6 U/mL, respectively. All patients who experienced noninfectious fever had anti‐PEG antibody titers above this cut‐off value (P = .033). An enzyme‐linked immunosorbent assay revealed that some anti‐PEG antibodies in patients with anti‐PEG antibody titers above the cut‐off value reacted with the PEGylated liposome. These results indicate the reactivity of the anti‐PEG antibodies to PEGylated therapeutics observed in hematologic patients and the possibility of the relationship between high titers of anti‐PEG antibodies and the development of adverse events after PEG‐G‐CSF administration.
A mixed methods study of autistic adults' mental health therapy experiences
Authors: Mazurek Micah O. , Pappagianopoulos Jessica , Brunt Sophie , Sadikova Eleonora , Nevill Rose , Menezes Michelle , Harkins Christina 
Published: January 2023

AbstractAutistic adults are at high risk for co‐occurring mental health problems and need access to effective and appropriate mental health treatment. However, the relative effectiveness or acceptability of specific mental health strategies among autistic adults has not been previously examined. The current study sought to gain a deeper understanding of autistic adults' experiences and preferences regarding mental health strategies using a mixed methods approach. Autistic adults (n = 303, ages 21–77) completed online surveys and open‐ended questions about their mental health and therapy experiences. Most (88.8%) had participated in therapy, with cognitive approaches being the most common. Regarding overall therapy experiences, qualitative analyses revealed four primary themes and nine subthemes. Therapist acceptance and understanding were seen as critical for therapy success, and many participants felt that therapy was helpful for personal growth. However, many participants found that talking in session was challenging and noted that aspects of the session format affected their ability to engage in therapy. Regarding specific strategies, four cross‐cutting themes and eight strategy‐specific subthemes were identified. A variety of strategies were seen as helpful for reducing anxiety and improving mood. However, autistic adults reported trouble generalizing strategies to daily life and found some techniques to be difficult to implement due, in part, to their unique autism‐related needs. As the first study of its kind, the results underscore the importance of establishing a safe and accepting therapeutic relationship, providing accommodations to support communication needs, and considering individual differences and preferences when selecting mental health strategies for autistic clients.
Autorepression‐Based Conditional Gene Expression System in Yeast for Variation‐Suppressed Control of Protein Dosage
Authors: Azizoğlu Aslı , Loureiro Cristina , Venetz Jonathan , Brent Roger 
Published: January 2023

AbstractConditional control of gene expression allows an experimenter to investigate many aspects of a gene's function. In the model organism Saccharomyces cerevisiae, a number of methods to control gene expression are widely practiced, including induction by metabolites, small molecules, and even light. However, all current methods suffer from at least one of a set of drawbacks, including need for specialized growth conditions, leaky expression, or requirement of specialized equipment. Here we describe protocols using two transformations to construct strains that carry a new controller in which all these drawbacks are overcome. In these strains, the expression of a controlled gene of interest is repressed by the bacterial repressor TetR and induced by anhydrotetracycline. TetR also regulates its own expression, creating an autorepression loop. This autorepression allows tight control of gene expression and protein dosage with low cell‐to‐cell variation in expression. A second repressor, TetR‐Tup1, prevents any leaky expression. We also present a protocol showing a particular workhorse application of such strains to generate synchronized cell populations. We turn off expression of the cell cycle regulator CDC20 completely, arresting the cell population, and then we turn it back on so that the synchronized cells resume cell cycle progression. This control system can be applied to any endogenous or exogenous gene for precise expression. © 2023 Wiley Periodicals LLC.Basic Protocol 1: Generating a parent WTC846 strainBasic Protocol 2: Generating a WTC846 strain with controlled expression of the targeted geneAlternate Protocol: CRISPR‐mediated promoter replacementBasic Protocol 3: Cell cycle synchronization/arrest and release using the WTC846‐K3::CDC20 strain
CPHEN‐15: Comprehensive phenotyping of human peripheral blood helper‐ILCs by flow cytometry
Authors: Del Zotto Genny , Vacca Paola , Moretta Lorenzo , Quatrini Linda 
Published: January 2023

AbstractInnate lymphoid cells (ILCs) comprise cytotoxic NK cells and helper‐ILCs, which are further divided in ILC1, ILC2, and ILC3. Helper‐ILCs mirror the effector functions of helper T‐cell subsets and contribute to host immune defense, tissue homeostasis and repair through cytokine secretion. Although they are mainly tissue‐resident, helper ILCs are also found in the peripheral blood (PB). In the human setting, it may be needed to analyze circulating helper ILCs to compare pathological to physiological conditions. In this review, we provide simple guidelines and a list of markers useful to study human PB helper ILCs phenotype and function by flow cytometry.
A study on the channel bonding in IoT networks: Requirements, applications, and challenges
Authors: Kandar Debdatta , Chyne Phidahunlang , Nath Sur Samarendra , Nandi Sukumar 
Published: January 2023

SummaryThe most well‐known sort of remote Internet connection is wireless local area networks (WLANs) due to its unsophisticated operation and deployment. Subsequently, the quantity of gadgets getting to the Internet through WLANs, for example, PCs, cell phones, or wearables, is expanding radically at the equivalent time that applications' throughput necessities do. To provide wireless networks with supplementary spectral resources, the researchers are considering the aggregation of frequency spectrums in licensed, unlicensed, and shared access (SA) bands. Channel aggregation/channel bonding (CA/CB) techniques accumulate quite a few channels together as one channel for the purpose of achieving better bandwidth utilization. In this study, we focus on reliable CA/CB techniques in different wireless networks. CA/CB procedures are utilized for empowering higher information rates by transmitting in more extensive channels, accordingly expanding range proficiency with the assured secure channel for communication. We also discuss the spectral scarcity issues in today's wireless IoT network. This paper presents an extensive survey on CA/CB procedures and methods, issues and challenges, and open research areas related to IoT devices. We analyze the performance of channel CA/CB strategies in the different wireless networks too.
Cultivated alien plants with high invasion potential are more likely to be traded online in China
Authors: Dong Ran , Dong Bi‐Cheng , Fu Qiu‐Yue , Yang Qiang , Dai Zhi‐Cong , Luo Fang‐Li , Gao Jun‐Qin , Yu Fei‐Hai , van Kleunen Mark 
Published: January 2023

AbstractBiological invasions have become a worldwide problem, and measures to efficiently prevent and control invasions are still in development. Like many other parts of the world, China is undergoing a dramatic increase in plant invasions. Most of the currently 933 established (i.e., naturalized) plant species, of which 214 are categorized as invasive, have been introduced into China for cultivation. It is likely that many of those species are still being traded, particularly online, by plant nurseries. However, studies assessing whether naturalized and invasive species are currently being traded more or less than nonnaturalized aliens are rare. We extracted online‐trade information for 13,718 cultivated alien plant taxa on 1688.com, the largest website for domestic B2B in China. We analyzed how the presence in online‐nursery catalogs, the number of online nurseries that offerred the species for sale, and the product type (i.e., seeds, live plants and vegetative organs) differed among nonnaturalized, naturalized noninvasive, and invasive species. Compared to nonnaturalized taxa, naturalized noninvasive and invasive taxa were 3.7–5.2 times more likely to be available for purchase. Naturalized noninvasive and invasive taxa were more frequently offered as seeds by online nurseries, whereas nonnaturalized taxa were more frequently offered as live plants. Based on these findings, we propose that, to reduce the further spread of invasive and potentially invasive plants, implementation of plant‐trade regulations and a monitoring system of the online horticultural supply chain will be essential.
Managing aquatic habitat structure for resilient trophic interactions
Authors: Ward Charlotte A. , Tunney Tyler D. , McCann Kevin S. 
Published: January 2023

AbstractStructural habitat (the three‐dimensional arrangement of physical matter, abiotic and biotic, at a location) is a foundational element for the resilience and maintenance of biodiversity, yet anthropogenic development is driving the global simplification of aquatic environments. Resource managers regularly seek to conserve aquatic food webs by increasing structural habitat complexity with expected benefits to fisheries; however, the global effectiveness of such actions is unclear. Our synthesis and theoretical analyses found that the response of a consumer–resource interaction (predatory sportfish and forage fish prey) to the addition of prey refuge habitat differed among systems with low and high rates of biomass transfer from resource to consumer (i.e., biomass potential); stabilization was not the rule. Greater prey refuge habitat availability tended to stabilize systems characterized by high biomass potential while simultaneously increasing consumer densities. In contrast, increasing prey refuge habitat availability in systems characterized by low biomass potential tended to mute energy transfer and moved consumer densities toward local extinction. Importantly, biomass potential and prey refuge can have antagonistic effects on stability and relative consumer densities, and it is therefore important to consider the local conditions of a system when using habitat manipulation as a management measure. Further development of our context‐dependent perspective to whole food webs, and across different environments, may help to guide structural habitat management to better restore and protect aquatic ecosystems.
Not out of the Mediterranean: Atlantic populations of the gorgonian Paramuricea clavata are a separate sister species under further lineage diversification
Authors: Coelho Márcio A. G. , Pearson Gareth A. , Boavida Joana R. H. , Paulo Diogo , Aurelle Didier , Arnaud‐Haond Sophie , Gómez‐Gras Daniel , Bensoussan Nathaniel , López‐Sendino Paula , Cerrano Carlo , Kipson Silvija , Bakran‐Petricioli Tatjana , Ferretti Eliana , Linares Cristina , Garrabou Joaquim , Serrão Ester A. , Ledoux Jean‐Baptiste 
Published: January 2023

AbstractThe accurate delimitation of species boundaries in nonbilaterian marine taxa is notoriously difficult, with consequences for many studies in ecology and evolution. Anthozoans are a diverse group of key structural organisms worldwide, but the lack of reliable morphological characters and informative genetic markers hampers our ability to understand species diversification. We investigated population differentiation and species limits in Atlantic (Iberian Peninsula) and Mediterranean lineages of the octocoral genus Paramuricea previously identified as P. clavata. We used a diverse set of molecular markers (microsatellites, RNA‐seq derived single‐copy orthologues [SCO] and mt‐mutS [mitochondrial barcode]) at 49 locations. Clear segregation of Atlantic and Mediterranean lineages was found with all markers. Species‐tree estimations based on SCO strongly supported these two clades as distinct, recently diverged sister species with incomplete lineage sorting, P. cf. grayi and P. clavata, respectively. Furthermore, a second putative (or ongoing) speciation event was detected in the Atlantic between two P. cf. grayi color morphotypes (yellow and purple) using SCO and supported by microsatellites. While segregating P. cf. grayi lineages showed considerable geographic structure, dominating circalittoral communities in southern (yellow) and western (purple) Portugal, their occurrence in sympatry at some localities suggests a degree of reproductive isolation. Overall, our results show that previous molecular and morphological studies have underestimated species diversity in Paramuricea occurring in the Iberian Peninsula, which has important implications for conservation planning. Finally, our findings validate the usefulness of phylotranscriptomics for resolving evolutionary relationships in octocorals.
Oxidorhenium(V) and Rhenium(III) Complexes with Arylselenolato and ‐tellurolato Ligands
Authors: Noschang Cabral Bruno , Fonseca Rodrigues Jéssica , Roca Jungfer Maximilian , Krebs Anke , Hagenbach Adelheid , Schulz Lang Ernesto , Abram Ulrich 
Published: January 2023

AbstractIn situ prepared lithium arylselenolates {LiSePh, LiSe(2,6‐Me2Ph), LiSe(2,4,6‐Me3Ph)} or ‐tellurolates {LiTePh, LiTe(2,6‐Me2Ph), LiTe(2,4,6‐Me3Ph)} react with [ReOCl3(PPh3)2] and (NBu4)Br in good yields under formation of rhenium(V) complexes of the composition (NBu4)[ReO(L)4)]. The first oxidorhenium(V) complex with four monodentate phenolato ligands, [ReO(2,6‐Me2Ph)4]−, was prepared and isolated in crystalline form. This allows a comparison of experimental and computational data of a full series of such compounds with basal O, S, Se and Te donor atoms. Similar reactions with [ReCl3(PPh3)2(CH3CN)] or [ReCl3(PMe2Ph)3] give trigonal bipyramidal rhenium(III) complexes of the compositions [Re(PPh3)(L)3(CH3CN)] or [Re(PPh3)2(L)3] depending on the chalcogenolate applied. The reported oxidorhenium(V) and rhenium(III) complexes were fully characterized by spectroscopic methods and X‐ray diffraction. The bonding situation in the rhenium(V) and rhenium(III) chalcogenolates was assessed through density functional theory calculations based on the quantum theory of atoms in molecules (QTAIM), natural bonding orbital (NBO) analysis, charge analysis, the electron localization function (ELF) maps and topological descriptors at the {3,−1} critical points such as metallicities. Expectedly, the ionicity of the rhenium‐chalcogen bonds decreases according to all three applied charge models from O to Te. Generally, the Re−Se and Re−Te bonds are more directional in the Re(III) complexes than in the oxidorhenium(V) compounds.
Index and mode modulated orthogonal frequency division multiplexing with enhanced spectral efficiency
Authors: Sengupta Ipsita , Dasgupta Shounak , Das Barman Abhirup 
Published: January 2023

AbstractIndex modulation aided orthogonal frequency division multiplexing (OFDM‐IM) is a potential multicarrier transmission technique for wireless data transmission systems due to its attractive error performance and energy efficiency. However, it fails to cope with increasing demand of high data rate in upcoming mobile data services, mainly, due to the lower spectral efficiency. In this communication, an efficient transmission technique, called index and mode modulation OFDM, is proposed that delivers additional information through permutations of multiple modes and indexed bits patterns resulting in higher spectral efficiency. For the proposed technique, a low complexity log‐likelihood ratio detector with suitable algorithm is designed and its computational complexity has been analysed. The simulation results indicate improved performance of this newly proposed scheme in terms of spectral efficiency, bit error rate, as well as energy efficiency over other existing index modulated and multiple mode based OFDM schemes, while maintaining considerably low computational complexity in detection. That proclaims the scheme as a potential transmission technique to meet the data transmission challenges in 5G and beyond.
Changes in fat uptake, color, texture, and sensory properties of Aloe vera gel‐coated eggplant rings during deep‐fat frying process
Authors: Varidi Mehdi , Ahmadzadeh‐Hashemi Saba , Nooshkam Majid 
Published: January 2023

AbstractThere is a widespread use of deep‐fat frying in both domestic and industrial sections, and deep‐fat fried foods are extremely popular due to their taste, color, and crispy texture. Human health can be, however, seriously compromised by the excessive consumption of oil, especially saturated fats and trans fatty acids. The use of hydrocolloids in inhibiting oil absorption has garnered considerable attention. This study was therefore aimed to lower the oil absorption in eggplant rings during the deep‐fat frying process with the aid of Aloe vera gel coating. The effects of gel concentration (0%, 50%, and 100%), frying time (2, 5, and 8 min), and frying temperature (160°C and 180°C) on the oil uptake, moisture content, texture, color, and sensory properties of the eggplant rings were evaluated. The gel coating led to a decrease in oil uptake (up to 50%), hardness (up to 0.98‐fold), ΔE (up to 0.89‐fold), and overall acceptance (up to 0.85‐fold), and an increase in moisture content (up to 1.47‐fold) and lightness (up to 1.14‐fold) of the samples. The frying time and temperature also influenced the physiochemical and sensory properties of the eggplant rings. The sample coated with 50% gel and fried at 180°C for 8 min had lower oil content and water loss with the highest acceptance rate in terms of taste, color, odor, texture, and appearance. The Aloe vera gel could be, therefore, a good candidate with high nutritional and economic value to reduce oil uptake in fried food products.
Associated biological and environmental factors of impaired executive function in preschool‐aged children: A population‐based study
Authors: Likhitweerawong Narueporn , Khorana Jiraporn , Boonchooduang Nonglak , Phinyo Phichayut , Patumanond Jayanton , Louthrenoo Orawan 
Published: January 2023

AbstractExecutive function (EF) is essential for academic achievement, successful work, and physical and mental health. Although evidence shows that several factors have been linked to EF, these results are mixed. This study aims to identify both biological and environmental variables associated with impaired EF in preschoolers. This study was a population‐based cross‐sectional study of healthy children aged 2–5 years from public and private schools in Chiang Mai, Thailand. EF was assessed using a parent report of the Behaviour Rating Inventory of Executive Function‐Preschool (BRIEF‐P). Multivariable logistic regression analysis was used to explore the biological and environmental factors relevant to impaired executive functioning. In a total of 1540 included children, 213 children (13.83%) were defined as impaired EF. We found that biological factors—male (odds ratio [OR], 1.41, 95% confidence interval [CI] [1.04–1.91]), overweight/obesity (OR, 1.59, 95% CI [1.10–2.31]), less physical activity (OR, 1.77, 95% CI [1.10–2.84]), and environmental factors—low maternal education (OR, 1.61, 95% CI [1.13–2.31]), permissive parenting style (OR, 2.75, 95% CI [1.24–6.13]), and excessive screen exposure (OR, 1.66, 95% CI [1.15–2.41])—are associated with an increased risk of impaired EF. Adjusting modifiable factors, such as healthy weight status, positive parenting, appropriate screen time, and adequate physical activities, may be necessary for children to promote EF.
Experience on trial of labor and vaginal delivery after two previous cesarean sections: A cohort study from a limited‐resource setting
Authors: Maroyi Raha , Nyakio Olivier , Buhendwa Cikwanine , Mukanga Omari , Kalunga Kiminyi , Kanyinda Kalala , Rukunghu Neema , Mukundane Byamungu , Kakusu Dieudonné , Mwilo Mambo , mbaya Eloge‐IIunga , Madarhi Cirhagane , Walala Boengandi , Kakisingi De joseph , Mukwege Denis 
Published: January 2023

AbstractObjectiveTo determine the success rate of trial of labor after two cesarean sections (TOLA2C) in the low‐resource setting of the Democratic Republic of Congo (DRC) and to describe factors associated with success and related complications.MethodsA prospective cohort study was conducted from 2015 to 2020 in a teaching hospital. Patients who underwent TOLA2C were followed across prenatal visits, onset of spontaneous labor, and delivery. Demographics and clinical characteristics were documented. Pearson and Fisher χ2 tests were used. Predictors of successful vaginal delivery were determined by logistic regression (P ˂ 0.05).ResultsAmong 532 patients, the success rate of TOLA2C was 405 (76.1%). Factors associated with success included birth spacing ≥24 months (adjOR: 2.02 ; 95% CI 1.14–3.56; P = 0.015), previous vaginal delivery (adjOR: 5.02; 95% CI 2.71–9.31; P ˂ 0.001), intercalated vaginal delivery (adjOR: 5.15; 95% CI 2.28–11.65; P ˂ 0.001), cervical dilation >6 cm (adjOR: 2.37; 95% CI 1.92–6.05; P = 0.031) and/or complete dilation on arrival in the delivery room (adjOR: 1.96; 95% CI 1.33–11.45; P = 0.047) and oxytocin stimulation (adjOR: 4.24; 95% CI 1.82–9.91; P ˂ 0.001). No association with hemorrhage, uterine rupture, transfer to neonatology, or maternal–neonatal deaths was observed.ConclusionsTOLA2C is possible in a low‐resource setting with a high success rate and low rates of complications. Patient selection and obstetrical team competency are required.