bluuebunny/crossref_metadata_2025_split huggingface dataset:
Winter Arctic Outflow Winds Cause Upper Ocean Cooling and Reoxygenation in a Temperate Canadian Fjord
Authors: Jackson J. M. , Holmes K. , Klymak J. M. , Bianucci L. , Evans W. , Floyd W. C. , Hannah C. G. , Hare A. , Wan D. 
Published: August 2023

AbstractArctic outflow winds bring cold air from the continent to the coastline through mountain passes. Using observational data and a 2‐D model, we show that a February 2019 outflow event caused the upper 100 m in Bute Inlet, British Columbia (within the traditional territory of the Homalco Nation) to cool up to 1.9°C and gain up to 4.1 mLL−1 of oxygen. The cold, oxygenated water persisted for almost 1 year within the 1,023–1,023.5 kgm−3 isopycnal range (∼50–150 m). Atmospheric (from 1929 to 2022) and oceanographic (from 1951 to 2022) data showed a statistically significant relationship between continental air temperature at Tatlayoko Lake and temperature and oxygen in Bute Inlet. This local mechanism that counters some effects of climate change could create a biological refugium as surrounding waters warm and lose oxygen at a faster rate. The number of outflow events decreased from 1951 to 2018, and increased since.
Space Plasma Diagnostics and Spacecraft Charging. The Impact of Plasma Inhomogeneities on Mutual Impedance Experiments
Authors: Bucciantini L. , Henri P. , Wattieaux G. , Lavorenti F. , Dazzi P. , Vallières X. 
Published: August 2023

AbstractPlasma diagnostic instruments are carried into space by satellites to measure in situ the properties of space plasmas. However, due to spacecraft charging, satellites perturb the surrounding plasma, that reacts by enveloping the platform and its instruments with a short scale, strongly inhomogeneous plasma region called plasma sheath. Such plasma sheath perturbs particles and electric field measurements performed onboard the satellite. Mutual Impedance (MI) experiments are a type of in situ diagnostic technique used in several space missions for the identification of the plasma density and the electron temperature. The technique is based on the electric coupling between emitting and receiving electric sensors embedded in the plasma to diagnose. Such sensors are surrounded by the plasma sheath, which is expected to affect the plasma response to MI emissions. In this context, we quantify for the first time the impact of the plasma sheath on the diagnostic performance of MI experiments. For this purpose, we use a full kinetic Vlasov‐Poisson model to simulate numerically MI experiments in an inhomogeneous medium. For the first time, we explain the locality of MI measurements. We find that MI plasma density diagnostic are not affected by the plasma sheath (dn/n < 10%). The experiment retrieves the density of the plasma unperturbed by the satellite's presence. The electron temperature diagnostic, instead, presents significant perturbations if the plasma sheath is ignored. To mitigate such electron temperature errors, the plasma sheath needs to be included in the analysis of MI measurements.
Insights Into NOx and HONO Chemistry in the Tropical Marine Boundary Layer at Cape Verde During the MarParCloud Campaign
Authors: Jiang Ying , Hoffmann Erik H. , Tilgner Andreas , Aiyuk Marvel B. E. , Andersen Simone T. , Wen Liang , van Pinxteren Manuela , Shen Hengqing , Xue Likun , Wang Wenxing , Herrmann Hartmut 
Published: August 2023

AbstractChemical processing of reactive nitrogen species, especially of NOx (= NO + NO2) and nitrous acid (HONO), determines the photochemical ozone production and oxidation capacity in the troposphere. However, sources of HONO and NOx in the remote marine atmosphere are still poorly understood. In this work, the multiphase chemistry mechanism CAPRAM in the model framework SPACCIM was used to study HONO formation at Cape Verde (CVAO) in October 2017, adopted with the input of current parameterizations for various HONO sources. Three simulations were performed that adequately reproduced ambient HONO levels and its diurnal pattern. The model performance for NOx and O3 improves significantly when considering dust‐surface‐photocatalytic conversions of reactive nitrogen compounds with high correlation coefficients up to 0.93, 0.56, and 0.89 for NO, NO2, and O3, respectively. Photocatalytic conversion of the adsorbed HNO3 on dust is modeled to be the predominant contributor for daytime HONO at CVAO, that is, accounting for about 62% of the chemical formation rate at noontime. In contrast, the ocean‐surface‐mediated conversion of NO2 to HONO and other discussed pathways are less important. The average OH levels at midday (9:00–16:00) modeled for cluster trajectory 1, 2, and 3 are 5.2, 5.1, and 5.2 × 106 molecules cm−3, respectively. Main OH formation is driven by O3 photolysis with a contribution of 74.6% to the total source rate, while HONO photolysis is negligible (∼1.8%). In summary, this study highlights the key role of dust aerosols for HONO formation and NOx cycling at CVAO and possibly in other dust‐affected regions, urgently calling for further investigations using field and model studies.
Glasdegib plus intensive or non-intensive chemotherapy for untreated acute myeloid leukemia: results from the randomized, phase 3 BRIGHT AML 1019 trial
Authors: Sekeres Mikkael A. , Montesinos Pau , Novak Jan , Wang Jianxiang , Jeyakumar Deepa , Tomlinson Benjamin , Mayer Jiri , Jou Erin , Robak Tadeusz , Taussig David C. , Dombret Hervé , Merchant Akil , Shaik Naveed , O’Brien Thomas , Roh Whijae , Liu Xueli , Ma Wendy , DiRienzo Christine G. , Chan Geoffrey , Cortes Jorge E. 
Published: August 2023

AbstractThis is the primary report of the randomized, placebo-controlled phase 3 BRIGHT AML 1019 clinical trial of glasdegib in combination with intensive chemotherapy (cytarabine and daunorubicin) or non-intensive chemotherapy (azacitidine) in patients with untreated acute myeloid leukemia. Overall survival (primary endpoint) was similar between the glasdegib and placebo arms in the intensive (n = 404; hazard ratio [HR] 1.05; 95% confidence interval [CI]: 0.782–1.408; two-sided p = 0.749) and non-intensive (n = 325; HR 0.99; 95% CI: 0.768–1.289; two-sided p = 0.969) studies. The proportion of patients who experienced treatment-emergent adverse events was similar for glasdegib versus placebo (intensive: 99.0% vs. 98.5%; non-intensive: 99.4% vs. 98.8%). The most common treatment-emergent adverse events were nausea, febrile neutropenia, and anemia in the intensive study and anemia, constipation, and nausea in the non-intensive study. The addition of glasdegib to either cytarabine and daunorubicin or azacitidine did not significantly improve overall survival and the primary efficacy endpoint for the BRIGHT AML 1019 phase 3 trial was not met. Clinical trial registration: ClinicalTrials.gov: NCT03416179.
Double-antibody-based nano-biosensing system for the onsite monitoring of SARS-CoV-2 variants
Authors: Hussein Heba A. , Kandeil Ahmed , Gomaa Mokhtar , Hassan Rabeay Y. A. 
Published: August 2023

AbstractThe fast and reliable diagnosis of COVID-19 is the foremost priority for promoting public health interventions. Therefore, double-antibody-based immunobiosensor chips were designed, constructed, and exploited for clinical diagnosis. Gold nanoparticles/tungsten oxide/carbon nanotubes (AuNPs/WO3/CNTs) were used as the active working sensor surface to support the chemical immobilization of a mixture of SARS-CoV-2 antibodies (anti-RBD-S and anti-RBD-S-anti-Llama monoclonal antibodies). The morphology and chemical functionalization of the fabricated disposable immunochips was characterized using scanning electron microscopy (SEM), Fourier transform infrared (FTIR) spectroscopy, cyclic voltammetry (CV), and electrochemical impedance spectroscopy (EIS). After full assay optimization, the immunobiosensor showed a high sensitivity to detect SARS-CoV-2-S protein with limits of detection and quantification of 1.8 and 5.6 pg/mL, respectively. On the other hand, for the SARS-CoV-2 whole virus particle analysis, the detection and quantification limits were determined to be 5.7 and 17 pg/mL, respectively. The biosensor showed a highly selective response toward SARS-CoV-2, even in the presence of influenza, nontargeting human coronaviruses, and Middle East respiratory syndrome coronavirus (MERS-CoV). The immunochips exhibited distinct responses toward the variants of concern: B.1>C.36.3>Omicron> Delta> Alpha coronavirus variants. For biosensor validation, twenty-nine clinical specimens were analyzed, and the impedimetric responses were positively detected for two Delta samples, eighteen Omicron samples, and six B.1-type samples in addition to three negative samples. Eventually, the immunobiosensor was fabricated in the form of ready-to-use chips capable of sensitive detection of virus variants, especially variants of concern (VOC) and interest, in a specimen within 15 min. The chips provided instantaneous detection with the direct application of clinical samples and are considered a point-of-care device that could be used in public places and hot spots.
Methylotrophic methanogens and bacteria synergistically demethylate dimethylarsenate in paddy soil and alleviate rice straighthead disease
Authors: Chen Chuan , Li Lingyan , Wang Yanfen , Dong Xiuzhu , Zhao Fang-Jie 
Published: August 2023

Abstract
Microorganisms play a key role in arsenic (As) biogeochemistry, transforming As species between inorganic and organic forms and different oxidation states. Microbial As methylation is enhanced in anoxic paddy soil, producing primarily dimethylarsenic (DMAs), which can cause rice straighthead disease and large yield losses. DMAs can also be demethylated in paddy soil, but the microorganisms driving this process remain unclear. In this study, we showed that the enrichment culture of methylotrophic methanogens from paddy soil demethylated pentavalent DMAs(V) efficiently. DMAs(V) was reduced to DMAs(III) before demethylation. 16S rRNA gene diversity and metagenomic analysis showed that Methanomassiliicoccus dominated in the enrichment culture, with Methanosarcina and Methanoculleus also being present. We isolated Methanomassiliicoccus luminyensis CZDD1 and Methanosarcina mazei CZ1 from the enrichment culture; the former could partially demethylate trivalent DMAs(III) but not DMAs(V) and the latter could demethylate neither. Addition of strain CZDD1 to the enrichment culture greatly accelerated DMAs(V) demethylation. Demethylation of DMAs(V) in the enrichment culture was suppressed by ampicillin, suggesting the involvement of bacteria. We isolated three anaerobic bacterial strains including Clostridium from the enrichment culture, which could produce hydrogen and reduce DMAs(V) to DMAs(III). Furthermore, augmentation of the Methanomassiliicoccus-Clostridium coculture to a paddy soil decreased DMAs accumulation by rice and alleviated straighthead disease. The results reveal a synergistic relationship whereby anaerobic bacteria reduce DMAs(V) to DMAs(III) for demethylation by Methanomassiliicoccus and also produce hydrogen to promote the growth of Methanomassiliicoccus; enhancing their populations in paddy soil can help alleviate rice straighthead disease.
Brain mediators of negative affect-induced physical symptom reporting in patients with functional somatic syndromes
Authors: Bogaerts Katleen , Van Den Houte Maaike , Jongen Daniëlle , Ly Huynh Giao , Coppens Eline , Schruers Koen , Van Diest Ilse , Jan Tack , Van Wambeke Peter , Petre Bogdan , Kragel Philip A. , Lindquist Martin A. , Wager Tor D. , Van Oudenhove Lukas , Van den Bergh Omer 
Published: August 2023

AbstractFunctional somatic syndromes (FSS) include fibromyalgia, irritable bowel syndrome (IBS), and others. In FSS patients, merely viewing negative affective pictures can elicit increased physical symptoms. Our aim was to investigate the neural mechanisms underlying such negative affect-induced physical symptoms in FSS patients. Thirty patients with fibromyalgia and/or IBS and 30 healthy controls (all women) watched neutral, positive and negative affective picture blocks during functional MRI scanning and rated negative affect and physical symptoms after every block. We compared brain-wide activation during negative versus neutral picture viewing in FSS patients versus controls using robust general linear model analysis. Further, we compared neurologic pain signature (NPS), stimulus intensity-independent pain signature (SIIPS) and picture-induced negative emotion signature (PINES) responses to the negative versus neutral affect contrast and investigated whether they mediated between-group differences in affective picture-induced physical symptom reporting. More physical symptoms were reported after viewing negative compared to neutral pictures, and this effect was larger in patients than controls (p = 0.025). Accordingly, patients showed stronger activation in somatosensory regions during negative versus neutral picture viewing. NPS, but not SIIPS nor PINES, responses were higher in patients than controls during negative versus neutral pictures (p = 0.026). These differential NPS responses partially mediated between-group differences in physical symptoms. In conclusion, picture-induced negative affect elicits physical symptoms in FSS patients as a result of activation of somatosensory and nociceptive brain patterns, supporting the idea that affect-driven alterations in processing of somatic signals is a critical mechanism underlying FSS.
Knockdown of SETD2 promotes erastin-induced ferroptosis in ccRCC
Authors: Xue Wei , Jian Wengang , Meng Yuyang , Wang Tengda , Cai Licheng , Yu Yongchun , Yu Yipeng , Xia Zhinan , Zhang Cheng 
Published: August 2023

AbstractClear cell renal cell carcinoma (ccRCC) is the most common subtype of kidney cancer and is associated with poor prognosis. The histone H3 lysine 36 methyltransferase SET-domain-containing 2 (SETD2) has been reported to be expressed at low levels and frequently mutated in ccRCC. Ferroptosis, a form of death distinct from apoptosis and necrosis, has been reported in recent years in renal cancer. However, the relationship between SETD2 and ferroptosis in renal cancer is not clear. Here, we demonstrated that SETD2 was expressed at low levels in ccRCC and was associated with poor prognosis. Moreover, we found that knockdown of SETD2 increased lipid peroxidation and Fe2+ levels in tumor cells, thereby increasing the sensitivity of erastin, a ferroptosis inducer. Mechanistically, histone H3 lysine 36 trimethylation (H3K36me3) which was catalyzed by SETD2, interacted with the promoter of ferrochelatase (FECH) to regulate its transcription and ferroptosis-related signaling pathways. In conclusion, the presesnt study revealed that knockdown of the epigenetic molecule, SETD2, significantly increases the sensitivity of ferroptosis inducers which promotes tumor cell death, thereby indicating that SETD2 may be a potential therapeutic target for ccRCC.
Erythropoietin re-wires cognition-associated transcriptional networks
Authors: Singh Manvendra , Zhao Ying , Gastaldi Vinicius Daguano , Wojcik Sonja M. , Curto Yasmina , Kawaguchi Riki , Merino Ricardo M. , Garcia-Agudo Laura Fernandez , Taschenberger Holger , Brose Nils , Geschwind Daniel , Nave Klaus-Armin , Ehrenreich Hannelore 
Published: August 2023

AbstractRecombinant human erythropoietin (rhEPO) has potent procognitive effects, likely hematopoiesis-independent, but underlying mechanisms and physiological role of brain-expressed EPO remained obscure. Here, we provide transcriptional hippocampal profiling of male mice treated with rhEPO. Based on ~108,000 single nuclei, we unmask multiple pyramidal lineages with their comprehensive molecular signatures. By temporal profiling and gene regulatory analysis, we build developmental trajectory of CA1 pyramidal neurons derived from multiple predecessor lineages and elucidate gene regulatory networks underlying their fate determination. With EPO as ‘tool’, we discover populations of newly differentiating pyramidal neurons, overpopulating to ~200% upon rhEPO with upregulation of genes crucial for neurodifferentiation, dendrite growth, synaptogenesis, memory formation, and cognition. Using a Cre-based approach to visually distinguish pre-existing from newly formed pyramidal neurons for patch-clamp recordings, we learn that rhEPO treatment differentially affects excitatory and inhibitory inputs. Our findings provide mechanistic insight into how EPO modulates neuronal functions and networks.
Linking emotional valence and anxiety in a mouse insula-amygdala circuit
Authors: Nicolas C. , Ju A. , Wu Y. , Eldirdiri H. , Delcasso S. , Couderc Y. , Fornari C. , Mitra A. , Supiot L. , Vérité A. , Masson M. , Rodriguez-Rozada S. , Jacky D. , Wiegert J. S. , Beyeler A. 
Published: August 2023

AbstractResponses of the insular cortex (IC) and amygdala to stimuli of positive and negative valence are altered in patients with anxiety disorders. However, neural coding of both anxiety and valence by IC neurons remains unknown. Using fiber photometry recordings in mice, we uncover a selective increase of activity in IC projection neurons of the anterior (aIC), but not posterior (pIC) section, when animals are exploring anxiogenic spaces, and this activity is proportional to the level of anxiety of mice. Neurons in aIC also respond to stimuli of positive and negative valence, and the strength of response to strong negative stimuli is proportional to mice levels of anxiety. Using ex vivo electrophysiology, we characterized the IC connection to the basolateral amygdala (BLA), and employed projection-specific optogenetics to reveal anxiogenic properties of aIC-BLA neurons. Finally, we identified that aIC-BLA neurons are activated in anxiogenic spaces, as well as in response to aversive stimuli, and that both activities are positively correlated. Altogether, we identified a common neurobiological substrate linking negative valence with anxiety-related information and behaviors, which provides a starting point to understand how alterations of these neural populations contribute to psychiatric disorders.
Environmental and genetic predictors of human cardiovascular ageing
Authors: Shah Mit , de A. Inácio Marco H. , Lu Chang , Schiratti Pierre-Raphaël , Zheng Sean L. , Clement Adam , de Marvao Antonio , Bai Wenjia , King Andrew P. , Ware James S. , Wilkins Martin R. , Mielke Johanna , Elci Eren , Kryukov Ivan , McGurk Kathryn A. , Bender Christian , Freitag Daniel F. , O’Regan Declan P. 
Published: August 2023

AbstractCardiovascular ageing is a process that begins early in life and leads to a progressive change in structure and decline in function due to accumulated damage across diverse cell types, tissues and organs contributing to multi-morbidity. Damaging biophysical, metabolic and immunological factors exceed endogenous repair mechanisms resulting in a pro-fibrotic state, cellular senescence and end-organ damage, however the genetic architecture of cardiovascular ageing is not known. Here we use machine learning approaches to quantify cardiovascular age from image-derived traits of vascular function, cardiac motion and myocardial fibrosis, as well as conduction traits from electrocardiograms, in 39,559 participants of UK Biobank. Cardiovascular ageing is found to be significantly associated with common or rare variants in genes regulating sarcomere homeostasis, myocardial immunomodulation, and tissue responses to biophysical stress. Ageing is accelerated by cardiometabolic risk factors and we also identify prescribed medications that are potential modifiers of ageing. Through large-scale modelling of ageing across multiple traits our results reveal insights into the mechanisms driving premature cardiovascular ageing and reveal potential molecular targets to attenuate age-related processes.
Contribution of boundary non-stoichiometry to the lower-temperature plasticity in high-pressure sintered boron carbide
Authors: Xu Haiyue , Ji Wei , Jiang Jiawei , Liu Junliang , Wang Hao , Zhang Fan , Yu Ruohan , Tu Bingtian , Zhang Jinyong , Zou Ji , Wang Weimin , Wu Jinsong , Fu Zhengyi 
Published: August 2023

AbstractThe improvement of non-oxide ceramic plasticity while maintaining the high-temperature strength is a great challenge through the classical strategy, which generally includes decreasing grain size to several nanometers or adding ductile binder phase. Here, we report that the plasticity of fully dense boron carbide (B4C) is greatly enhanced due to the boundary non-stoichiometry induced by high-pressure sintering technology. The effect decreases the plastic deformation temperature of B4C by 200 °C compared to that of conventionally-sintered specimens. Promoted grain boundary diffusion is found to enhance grain boundary sliding, which dominate the lower-temperature plasticity. In addition, the as-produced specimen maintains extraordinary strength before the occurrence of plasticity. The study provides an efficient strategy by boundary chemical change to facilitate the plasticity of ceramic materials.
Omicron infection following vaccination enhances a broad spectrum of immune responses dependent on infection history
Authors: Hornsby Hailey , Nicols Alexander R. , Longet Stephanie , Liu Chang , Tomic Adriana , Angyal Adrienn , Kronsteiner Barbara , Tyerman Jessica K. , Tipton Tom , Zhang Peijun , Gallis Marta , Supasa Piyada , Selvaraj Muneeswaran , Abraham Priyanka , Neale Isabel , Ali Mohammad , Barratt Natalie A. , Nell Jeremy M. , Gustafsson Lotta , Strickland Scarlett , Grouneva Irina , Rostron Timothy , Moore Shona C. , Hering Luisa M. , Dobson Susan L. , Bibi Sagida , Mongkolsapaya Juthathip , Lambe Teresa , Wootton Dan , Hall Victoria , Hopkins Susan , Dong Tao , Barnes Eleanor , Screaton Gavin ,   The PITCH Consortium, Richter Alex , Turtle Lance , Rowland-Jones Sarah L. , Carroll Miles , Duncan Christopher J. A. , Klenerman Paul , Dunachie Susanna J. , Payne Rebecca P. , de Silva Thushan I. 
Published: August 2023

AbstractPronounced immune escape by the SARS-CoV-2 Omicron variant has resulted in many individuals possessing hybrid immunity, generated through a combination of vaccination and infection. Concerns have been raised that omicron breakthrough infections in triple-vaccinated individuals result in poor induction of omicron-specific immunity, and that prior SARS-CoV-2 infection is associated with immune dampening. Taking a broad and comprehensive approach, we characterize mucosal and blood immunity to spike and non-spike antigens following BA.1/BA.2 infections in triple mRNA-vaccinated individuals, with and without prior SARS-CoV-2 infection. We find that most individuals increase BA.1/BA.2/BA.5-specific neutralizing antibodies following infection, but confirm that the magnitude of increase and post-omicron titres are higher in the infection-naive. In contrast, significant increases in nasal responses, including neutralizing activity against BA.5 spike, are seen regardless of infection history. Spike-specific T cells increase only in infection-naive vaccinees; however, post-omicron T cell responses are significantly higher in the previously-infected, who display a maximally induced response with a highly cytotoxic CD8+ phenotype following their 3rd mRNA vaccine dose. Responses to non-spike antigens increase significantly regardless of prior infection status. These findings suggest that hybrid immunity induced by omicron breakthrough infections is characterized by significant immune enhancement that can help protect against future omicron variants.