bluuebunny/crossref_metadata_2025_split huggingface dataset:
Protein complexes in cells by AI‐assisted structural proteomics
Authors: O'Reilly Francis J , Graziadei Andrea , Forbrig Christian , Bremenkamp Rica , Charles Kristine , Lenz Swantje , Elfmann Christoph , Fischer Lutz , Stülke Jörg , Rappsilber Juri 
Published: February 2023

AbstractAccurately modeling the structures of proteins and their complexes using artificial intelligence is revolutionizing molecular biology. Experimental data enable a candidate‐based approach to systematically model novel protein assemblies. Here, we use a combination of in‐cell crosslinking mass spectrometry and co‐fractionation mass spectrometry (CoFrac‐MS) to identify protein–protein interactions in the model Gram‐positive bacterium Bacillus subtilis. We show that crosslinking interactions prior to cell lysis reveals protein interactions that are often lost upon cell lysis. We predict the structures of these protein interactions and others in the SubtiWiki database with AlphaFold‐Multimer and, after controlling for the false‐positive rate of the predictions, we propose novel structural models of 153 dimeric and 14 trimeric protein assemblies. Crosslinking MS data independently validates the AlphaFold predictions and scoring. We report and validate novel interactors of central cellular machineries that include the ribosome, RNA polymerase, and pyruvate dehydrogenase, assigning function to several uncharacterized proteins. Our approach uncovers protein–protein interactions inside intact cells, provides structural insight into their interaction interfaces, and is applicable to genetically intractable organisms, including pathogenic bacteria.
False-positive 123I-metaiodobenzylguanidine scintigraphy in a patient with a gastrointestinal stromal tumor presenting as a left adrenal incidentaloma
Authors: Sugito Erika , Tanabe Akiyo , Maruyama Koji , Nohara Kyoko , Enomoto Naoki , Bouchi Ryotaro , Ohsugi Mitsuru , Ueki Kohjiro , Yamada Kazuhiko , Kajio Hiroshi 
Published: February 2023

Summary
A 47-year-old man was diagnosed with a left adrenal incidentaloma at 40 years of age. The tumor had irregular margins and grew from 18 mm to 30 mm in maximum diameter over 7 years. On computed tomography scan, the mass appeared to localize within the tip of the lateral limb of the left adrenal gland, and between the left adrenal gland and the posterior wall of the stomach. The plasma corticotropin and cortisol concentrations and the 24-h urine fractionated metanephrine levels were normal. 123I-metaiodobenzylguanidine scintigraphy showed tumor avidity consistent with a hormonally inactive pheochromocytoma. A laparoscopic left adrenalectomy was performed; however, no tumor was present in the resected specimen. Abdominal computed tomography postoperatively showed that the tumor remained intact and appeared to connect to the posterior wall of the stomach. A laparotomy was performed and the tumor was removed. The tumor was localized to the intraperitoneal space and isolated from the posterior wall of the stomach. The pathological diagnosis was a gastrointestinal stromal tumor. Clinicians need to be aware of the limitations of diagnostic imaging studies in diagnosing non-functioning adrenal incidentalomas, which require a pathological analysis for the final diagnosis. Moreover, clinicians need to provide patients with sufficient informed consent when deciding on treatment strategies.

Learning points

Anatomic structures and tumors that develop in neighboring tissues to the adrenal glands may be confused with primary adrenal tumors.

123I- metaiodobenzylguanidine (MIBG) scintigraphy is specific for diagnosing pheochromocytomas and paragangliomas; however, it has been reported that 123I-MIBG may accumulate in neuroendocrine tumors as well as other tumors.

Clinicians should recognize the limitations of imaging studies and the uncertainty of an imaging-based preoperative diagnosis.
X-ray diffractometric study of HDPE/GaAs and HDPE/GaAs<Te> composites
Authors: Gadzhieva N.N. , Ahmadova G.B. , Melikova S.Z. , Asadov F.G. 
Published: February 2023

High-density polyethylene sheets (HDPE), HDPE/GaAs and HDPE/ GaAs<Te> composites with GaAs and GaAs<Te> semiconductor fillers were studied by X-ray diffractometry at room temperature. The degree of crystallization of these samples was calculated and it was determined that the inclusion of fillers in the polymer matrix (x=1-10% composite) leads to an increase in the degree of crystallization by 1.3-1.4 times. The obtained results are explained by the change of the upper molecular structure of the polymer.
The Nexus between individual interest and school engagement in bolstering Physical Culture for a habitual healthy régime: a case of a state university
Authors: Bautista Clarenz , De Dios Don Al , Lobo Joseph 
Published: February 2023

Background and Study Aim. A worldwide consensus exists that students' levels of interest strongly predict and trigger their engagement in various activities, particularly those that promote physical culture. Academic works in the field of Physical Education have demonstrated that students can be extremely engaged when they are exposed to situations that pique their interest. However, the relationship and influence of students’ individual interests on their engagement in school have not yet been documented further. Hence, conducting an investigation should be performed.
Materials and Methods. The current research endeavors to identify any discernible gender differences in students' individual interests and school engagement levels. In addition, the initiative intends to use an analysis of the relationship between individual interest and school engagement as a foundation for promoting a healthy and active campus community. Undergraduate students in their 1st- and 2nd-year who are enrolled in Physical Education 1 and Physical Education 3 courses during the 1st Semester of the Academic year 2022-2023 are the participants in the study. Participants were selected via Purposive and Convenience Sampling Techniques. The data for this study was collected through a Google forms-based online survey. Additionally, the published English version of the Students’ Individual Interest in Physical Education questionnaire and the Utrecht Work Engagement Scale for Students (UWES-9S) were adopted to gather data from the respondents. In order to examine the difference between individual interest and school engagement according to gender, Independent Samples T-Test was performed. Spearman Rho’s and Multiple Regression Analysis examined the relationship between the two variables.
Results. After obtaining data from 1072 university students [(Nmale = 456(42.5%), Nfemale = 616(57.5%)], it was found that no significant variance was observed concerning individual interest and school engagement based on gender. Additionally, a positive and significant association was observed between individual interest and school engagement. Lastly, it was also found that individual interest, alongside its three factors, predicts school engagement.
Conclusions. Based on the findings, it can be concluded that students’ individual interests have a direct and beneficial influence on their level of school engagement. This investigation was conducted to fully communicate physical culture to students in order to improve their overall well-being. Based on the findings, recommendations for the future research direction and physical education teachers have been produced as a result of this investigation.
The effects of massed and distributed drills, muscle strength, and intelligence quotients towards tennis groundstroke skills of sport students
Authors: Nugroho Djoko , Hidayatullah Mohammad F. , Doewes Muchsin , Purnama Sapta K. 
Published: February 2023

Background and Study Aim. Basic forehand and backhand technical skills are the main requirements that must be mastered in playing tennis. Physical condition and intellectual intelligence were found to be the factors that affect the quality of tennis. On the other hand, limited learning time, and the number of teaching staff and facilities are classic challenges in the implementation of learning. This study describes the different effects of massed and distributed exercise, arm strength, and intellectual on the forehand and backhand skills of sports students.
Material and Methods. A quasi-experimental method with two group pretest and posttest design approached the 64 volunteers of male sports students (age 19.3±1.7, BMI 20.17±1.47), who had attended the tennis course. The sample is divided into 2 groups of Massed Practices (MP) and Distributed Practices (DP) according to the score of the upper-arm strength and intelligence test. The anthropometrics were evaluated through digital microtome stature, the arm strength was evaluated with a push-up test and the kinesthetic perception was confirmed with the intelligence quotient (IQ) test. The prerequisite test employed Kolmogorov-Smirnov, while Bivariate analysis utilized the Independent Sample T-test and Paired Sample T-test of the SPSS 20.0 version.
Results. The study showed that MP and DP had different positive contribution values to the tennis drive (p=0.003, p<0.05), while distributed gave a better contribution to the tennis drive with a significant value (p=0.001, p<0.05). The high arm muscle strength provides high accuracy in groundstroke (p=0.003, p<0.05), also for the high score on the intelligence test significantly affect the accuracy of tennis strokes (p=0.000, p<0.05).
Conclusions. The results showed that there are differences in exercise methods, arm muscle strength, and intelligence quotient against tennis drive punches.
Validity and reliability of an assessment instrument of track start in swimming
Authors: Madureira Fabrício , Gomes Thábata V.B. , Oliveira Thiago A.C. , Freudenheim Andrea M. , Corrêa Umberto C. 
Published: February 2023

Background and Study Aim. The development and validation of assessment instruments are essential procedures for any professional intervention, including in physical education and sports contexts. This study aimed to provide a component-based assessment instrument for track start in swimming. It refers to the act of starting the competitive swim by diving into the water from a raised block (platform) at the edge of the pool.
Material and Methods. The content validity involved the participation of sixty-three experienced swimming coaches. They completed a questionnaire about their degree of agreement (Likert scale) with the initial position, impulsion, flight, and entry into the water as the track start's components. Reliability and construct validity involved the participation of seven experienced swimming coaches. The first was verified through test-retest (intrarater) and reproducibility (interrater) procedures. The latter was tested by considering the instrument's ability to assess different track start performances.
Results. The concordant answers (strongly agree and agree) ranged from 63% to 95%, with most components above 80%. The Intraclass Correlation Coefficients (ICC) results show from good to excellent interrater and intrarater reliabilities. Specifically, the interrater correlations in the test and intrarater correlations in track start 1 were revealed excellent. In addition, the interrater correlations in the retest and intrarater correlations in track start 2 were revealed good.
Conclusions. The obtained validities and reliabilities made possible a component-based assessment instrument that allows: (i) a clear and standardized structure of track start in swimming involving the initial position, impulsion, flight, and entry into the water; (ii) accessing to the practitioners’ states in relation to desired start performance; and (iii) a reference for coaches and teachers to provide instruction and feedback for learning and performance improvement.
Cognitive functions and special working capacity in elite boxers
Authors: Korobeynikov Georgiy , Korobeinikova Lesia , Raab Markus , Baić Mario , Borysova Olga , Korobeinikova Ivanna , Shengpeng Gou , Khmelnitska Irene 
Published: February 2023

Background and Study Aim. In the basis of the boxer's power qualities is the ability to perceive and process external information. Therefore, in order to ensure the proper training of qualified boxers, there is a need to monitor the functional state of the boxer’s organism. The relationship between cognitive functions and special working capacity of elite boxers were studied.
Material and Methods. The level of special working capacity was studied by the test of punching a boxer for 8 s at maximum speed. Cognitive functions (verbal and nonverbal intelligence, and word memory) were studied using the "Multipsychometer-05" hardware-software. 26 elite boxers, aged 18-24, were tested.
Results. The research results showed that groups of boxers with different levels of special working capacity differ in terms of accuracy and efficiency when performing a cognitive task with verbal stimuli. The resulted that the high level of special working capacity of elite boxers is provided by the manifestation of verbal intelligence. Besides, the special working capacity is related with activation of mental functions of attention, speed of visual perception, operational and logical thinking. At the same time, significantly higher values of decision latency in this group of boxers indicate a slowdown in the processing of nonverbal information in boxers with a high level of special working capacity to work. It can be noted that the special working capacity of boxers has a direct relationship with quality and inverse relationship with the speed of information processing. The speed of memorizing verbal information is better in boxers with a high level of special working capacity. This fact indicates the importance of the verbal factor for the assimilation of special information in the training of boxers. The best values of accuracy and efficiency indicate the relationship of special working capacity in skilled boxers with the quality and volume of memorized verbal information.
Conclusions. The special working capacity of elite boxers has a direct relationship with quality and inverse relationship with the speed of information processing. The elite boxers with a high level of special working capacity have a better speed of verbal information memorizing.
Optical modeling of a radioisotope atmospheric chamber
Authors: Hubbard Lance , Ritzmann Andrew , Wahl Jon , Shilling John E. , Soderquist Chuck , Abrecht David , Smith Nathaniel 
Published: February 2023

AbstractVolatile radioisotopes represent a substantial health risk when released into the environment. To better understand the environmental fate of radioisotopes, the authors constructed a cylindrical steel-walled chamber to simulate the atmospheric processing of volatile radioactive gases. Optical modeling was performed for the properties of simulated sunlight in the chamber to better characterize atmospheric reaction studies. Optical simulations were performed using two wall materials (steel and thin-film silica) and validated against experimental measurements. This optical analysis methodology can be used to improve the fidelity of atmospheric models by accounting for optical inhomogeneities enabling a firmer grasp of radioisotopes’ environmental fate.
Graphical abstract
Endobronchial surfactant administration in full-term newborn with respiratory distress syndrome
Authors: Ivanov Dmitriy O. , Kiriakov Kirill S. , Pshenisnov Konstantin V. , Tesaeva Imani A. , Nasipova Milana U. , Sizaeva Elena A. , Gabisov Azamat T. 
Published: February 2023

Neonatal respiratory distress syndrome is the leading cause of neonatal acute respiratory failure. Despite the successes achieved and the existing international recommendations, in some cases there is a severe course of this disease, which requires a personalized approach to the patient and intensive care measures.
The article presents a case of successful treatment of acute respiratory distress syndrome in a full-term newborn complicated by pulmonary barotrauma using monobronchial administration of exogenous surfactant under X-ray control. In order to assess the course of the disease and the effectiveness of treatment, a retrospective analysis of medical documentation was carried out. From the first minutes of life, the child had respiratory disorders, which was the basis for non-invasive mechanical lung ventilation nCPAP (nasal continuous positive airway pressure). Progression of hypercapnia and hypoxemia was revealed over time, and therefore tracheal intubation was performed and convective mechanical ventilation was started with FiO2 = 1,0. Monobronchial administration of exogenous surfactant was a key element of the therapy that allowed to achieve stabilization of the condition and regression of gas exchange disorders with complete recovery of the patient.
Monobronchial administration of surfactant in acute respiratory distress syndrome with heterogeneous lung involvement is an effective treatment option and can be used in clinical practice for refractory hypoxemia.
Biological Invasion Threat to Wetlands IN Urban Areas of White River, Mbombela in Mpumalanga Province, South Africa: A Case Study
Authors: Mafuwane Hluphi Constance , Muchie Mammo 
Published: February 2023

Purpose: Wetlands are assets in the country, and they help make cities lockable and attractive. The study aims to assess the spread of alien invasive plants that affect the wetlands and construct a policy framework that could be used to preserve and conserve groundwater resources (Wetlands) in urban areas of Mpumalanga Province, specifically White River. This research will answer why alien invasives are a threat to wetlands.
Methodology: The study was conducted in White River on Longtom Street. A single observer conducted fixed-width line transect surveys to investigate species richness and abundance for all the untreated species detected next to the wetland. Three transects per habitat type were visited twice through twice per week.
Main Findings: During the survey, seven different alien invasive species were observed. The leading species was Solanum mauritianum, followed by Chromolaena odorata species; the third most dominant species was Lantana camara and Tecoma stans. Most species observed were illegally dumped by the residents that stay closer to the wetland, and no awareness, information, and training about the importance of wetlands were provided to the residents.
Implications: The framework can do more to help local communities manage urban wetlands, identifying opportunities for mapping and functional assessment to improve restoration and protection efforts. It can facilitate research and peer-to-peer exchange on innovative funding and financing methods for nature-based projects.
Novelty: Findings and recommendations resulting from this study will be summarized into a strategic guide by the end of 2023. It is recommended that all alien invasive plants in the wetland be eradicated. Wetlands must be protected to improve human health and well-being.
Technical and environmental features of the application of renewable energy for decentralized power supply zones
Authors: Dolgikh N. N. , Osipov D. S. , Osipova N. D. 
Published: February 2023

A feature of the geographical location of the Khanty-Mansiysk Autonomous Okrug - Yugra is the presence of a large number of zones of decentralized power supply. For a comparative environmental assessment of renewable energy installations, it is necessary to take into account the emissions of the entire life cycle. Throughout the presented cycle from mining to the production of power plant structures and then the disposal of the facility, a significant part of CO2 emissions is present. The problem of dismantling and recycling of spent structures of wind power plants is becoming essential. Wind turbines cause the death of birds, violate the conditions of comfortable living for people and animals
From a technical point of view, it is necessary to take into account the regime parameters: indicators of the quality of electricity at the point of connection, voltage levels at load nodes, operating modes of energy storage devices. To assess the operating parameters of an isolated power supply system with renewable energy sources, this paper proposes to use the wavelet transform method. The Haar wavelet was used as a basic function in the paper. A mathematical model is presented that makes it possible to obtain a low-frequency (trend) component and a high-frequency component using the wavelet transform. The model allows for the optimal choice of a hybrid energy storage device for a renewable energy source - a battery and a supercapacitor.
Factores intrínsecos a la sobrecarga laboral en el estrés del profesorado
Authors: Suárez Oscar , Suárez-Riveros Lilian , Lizarazo-Osorio Julio 
Published: February 2023

El objetivo de este estudio fue encontrar los factores intrínsecos al estrés laboral del profesorado activo en Colombia, México y Perú, distintos a la sobrecarga laboral (SL). Además, buscó establecer, con un modelo no supervisado, cómo los factores identificados predicen la SL. El enfoque fue principalmente cuantitativo, apoyado en el análisis de componentes principales y en la regresión lineal por introducción para construir el modelo. El instrumento indagó las categorías: emociones laborales, demandas laborales, recursos laborales, burnout y recursos tecnológicos para la actividad laboral. Los resultados muestran un alfa global de 0,856. Se obtuvieron diez factores intrínsecos que explican el 71,331 % de la varianza, de los cuales, seis contribuyen a predecir la SL: trabajo en casa, apoyo social, resiliencia didáctica tecnológica, motivación del estudiante, autocontrol emocional e incertidumbre laboral. A manera de conclusión, se encontró que el profesorado tuvo la capacidad resiliente tecnológica y didáctica, que le permitió adaptarse a la presencialidad remota; y que el trabajo colaborativo entre docentes contribuye a disminuir el estrés académico por SL. No obstante, la incertidumbre laboral incide en la SL.
Entropic Dynamics in a Theoretical Framework for Biosystems
Authors: Summers Richard L 
Published: February 2023

Central to an understanding of the physical nature of biosystems is an apprehension of their ability to control entropy dynamics in their environment. To achieve ongoing stability and survival, living systems must adaptively respond to incoming information signals concerning matter and energy perturbations in their biological continuum (biocontinuum). Entropy dynamics for the living system are then determined by the natural drive for reconciliation of these information divergences in the context of the constraints formed by the geometry of the biocontinuum information space. The configuration of this information geometry is determined by the inherent biological structure, processes and adaptive controls that are necessary for the stable functioning of the organism. The trajectory of this adaptive reconciliation process can be described by an information-theoretic formulation of the living system’s procedure for actionable knowledge acquisition that incorporates the axiomatic inference of the Kullback Principle of Minimum Information Discrimination (a derivative of Jaynes’ principle of maximal entropy). Utilizing relative information for entropic inference provides for the incorporation of a background of the adaptive constraints in biosystems within the operations of Fisher biologic replicator dynamics. This mathematical expression for entropic dynamics within the biocontinuum may then serve as a theoretical framework for the general analysis of biological phenomena.