bluuebunny/crossref_metadata_2025_split huggingface dataset:
Primary Pancreatic Lymphoma Masquerading as Carcinoma on 18F-FDG PET/CT
Authors: Chen Yun , Zheng Shan , Wu Zenan , Lin Yanmin , Miao Weibing 
Published: July 2023

Abstract
A 76-year-old man with yellowish discoloration of sclera and skin for 2 months was referred to 18F-FDG PET/CT for metabolic characterization of the mass in the pancreas. The images showed intense FDG uptake in the head of the pancreas, as well as a lymph nodal mass in the hepatic hilar region, which was consistent with pancreatic malignancy. Histopathologic findings showed characteristic findings of diffuse large B-cell lymphoma with no evidence of adenocarcinoma.
Temperature acclimation of photosynthesis and carbohydrate metabolism are related to the geographical origin ofArabidopsis thaliana
Authors: Hernandez Jakob Sebastian , Dziubek Dejan , Schröder Laura , Seydel Charlotte , Kitashova Anastasia , Brodsky Vladimir , Nägele Thomas 
Published: July 2023

AbstractAcclimation is a multigenic trait by which plants adjust photosynthesis and metabolism to cope with a changing environment. Here, natural variation of photosynthetic and metabolic acclimation was analyzed in response to low and elevated temperature. For this, 18 natural accessions ofArabidopsis thaliana,originating from Africa and Europe, were grown at 22°C before being exposed to 4°C and 34°C for cold and heat acclimation, respectively. Amounts of carbohydrates were quantified together with their subcellular distribution across plastids, cytosol and vacuole. Linear electron transport rates (ETRs) were determined together with maximum quantum efficiency of photosystem II (Fv/Fm) for all growth conditions and under temperature fluctuation. Under elevated temperature, residuals of ETR under increasing photosynthetic photon flux densities were found to significantly correlate with the longitudinal gradient of the geographic origin of accessions indicating a naturally occurring east-west gradient of photosynthetic acclimation capacities. Further, in heat acclimated plants, vacuolar fructose amount was found to positively correlate with longitude while plastidial and cytosolic amounts were found to be negatively correlated. Plastidial sucrose concentrations were found to positively correlate with maximal ETRs under fluctuating temperature indicating a stabilizing role within the chloroplast. In summary, our findings revealed specific subcellular carbohydrate distributions which contribute differentially to photosynthetic efficiencies of naturalArabidopsis thalianaaccessions across a longitudinal gradient. This sheds light on the relevance of subcellular metabolic regulation for photosynthetic performance in a fluctuating environment and supports the physiological interpretation of naturally occurring genetic variation of temperature tolerance and acclimation.
Distinct forms of structural plasticity of adult-born interneuron spines induced by different odor learning paradigms
Authors: Ferreira Aymeric , Constantinescu Vlad-Stefan , Malvaut Sarah , Saghatelyan Armen , Hardy Simon V. 
Published: July 2023

AbstractDuring development and in adulthood the morpho-functional properties of neural networks constantly adapt in response to environmental stimuli and learned experiences. One of the processes that allows neuronal networks to be constantly reshaped is synaptic plasticity, which is induced in response to sensory experience and learning. Synaptic plasticity allows for the formation/elimination of synaptic connections as well as the strengthening of pre-existing ones. The olfactory system is particularly prone to constant morpho-functional reshaping of neural networks and synaptic rewiring throughout the lifespan of an animal, mainly because of the presence of continuous neurogenesis in the olfactory bulb (OB). This constant synaptic rewiring brought by adult-born neurons is modulated by the level of odor-induced activity and olfactory learning. It remains, however, unclear whether the complexity of distinct odor-induced learning paradigms and sensory stimulation induces different forms of structural plasticity. In the present study, we developed an analytical pipeline to perform 3D reconstructions of spines from confocal images followed by clustering of reconstructed spines based on different morphometric features and in relationship with different sensory stimuli and learning paradigms. We show that while sensory deprivation decreased the overall density of adult-born neurons in the OB without any noticeable changes in the morphometric properties of these spines, simple and complex odor learning paradigms triggered distinct forms of structural plasticity. A simple odor learning task affected the morphometric properties of the spines without any changes in spine density, whereas a complex odor learning task induced changes in spine density, without substantial changes in the morphology of the spines. Our work reveals the vast panoply of distinct forms of synaptic plasticity of adult-born neurons in the OB tailored to the complexity of odor-learning paradigms and sensory inputs.
Contribution of the delta-class glutathione S-transferase to agrochemical adaptation inApis mellifera
Authors: Moural Timothy W. , Koirala B K Sonu , Bhattarai Gaurab , He Ziming , Guo Haoyang , Phan Ngoc T. , Rajotte Edwin G. , Biddinger David J. , Hoover Kelli , Zhu Fang 
Published: July 2023

AbstractThe European honey bee,Apis mellifera, serves as the principle managed pollinator species globally. In recent decades, honey bee populations have been facing serious health threats from combined biotic and abiotic stressors, including diseases, limited nutrition, and agrochemical exposure. Understanding the molecular mechanisms underlying xenobiotic adaptation ofA. melliferais critical, considering its extensive exposure to phytochemicals and agrochemicals present in flowers, propolis, hives, and the environment. In this study, we conducted a comprehensive structural and functional characterization of AmGSTD1, a delta class glutathione S-transferase (GST) enzyme, to unravel its roles in agrochemical detoxification and antioxidative stress responses. Significantly, we determined the 3D structure of a honey bee GST using protein crystallography for the first time, providing new insights into its molecular structure. Our investigations revealed that AmGSTD1 efficiently metabolizes model substrates, including 1-chloro-2,4-dinitrobenzene (CDNB), p-nitrophenyl acetate (PNA), phenylethyl isothiocyanate (PEITC), propyl isothiocyanate (PITC), and the oxidation byproduct 4-hydroxynonenal (4-HNE). Moreover, we discovered that AmGSTD1 exhibits binding affinity with the fluorophore 8-Anilinonaphthalene-1-sulfonic acid (ANS), which can be inhibited with various herbicides, fungicides, insecticides, and their metabolites. These findings highlight the potential contribution of AmGSTD1 in safeguarding honey bee health against various agrochemicals and their metabolites, while also mitigating oxidative stress resulting from exposure to these substances.
Glial-derived mitochondrial signals impact neuronal proteostasis and aging
Authors: Bar-Ziv Raz , Dutta Naibedya , Hruby Adam , Sukarto Edward , Averbukh Maxim , Alcala Athena , Henderson Hope R. , Durieux Jenni , Tronnes Sarah U. , Ahmad Qazi , Bolas Theodore , Perez Joel , Dishart Julian G. , Vega Matthew , Garcia Gilberto , Higuchi-Sanabria Ryo , Dillin Andrew 
Published: July 2023

AbstractThe nervous system plays a critical role in maintaining whole-organism homeostasis; neurons experiencing mitochondrial stress can coordinate the induction of protective cellular pathways, such as the mitochondrial unfolded protein response (UPRMT), between tissues. However, these studies largely ignored non-neuronal cells of the nervous system. Here, we found that UPRMTactivation in four, astrocyte-like glial cells in the nematode,C. elegans, can promote protein homeostasis by alleviating protein aggregation in neurons. Surprisingly, we find that glial cells utilize small clear vesicles (SCVs) to signal to neurons, which then relay the signal to the periphery using dense-core vesicles (DCVs). This work underlines the importance of glia in establishing and regulating protein homeostasis within the nervous system, which can then impact neuron-mediated effects in organismal homeostasis and longevity.One-Sentence SummaryGlial cells sense mitochondrial stress and signal a beneficial stress signal to promote neuronal health and longevity.
Anti-apoptotic and anti-inflammatory properties of grapefruit IntegroPectin on human microglial HMC3 cell line
Authors: Scordino Miriana , Urone Giulia , Frinchi Monica , Valenza Chiara , Bonura Angela , Cipollina Chiara , Ciriminna Rosaria , Meneguzzo Francesco , Pagliaro Mario , Mudò Giuseppa , Di Liberto Valentina 
Published: July 2023

AbstractBackground and aimDespite the intense research, most therapeutic strategies failed in preventing or treating neurodegenerative diseases, characterized by a combination of chronic neurodegeneration, oxidative stress and neuroinflammation. The broad protective activity of IntegroPectin derived from industrial waste grapefruit peel via hydrodynamic cavitation has been recently characterized. In this study, we investigated the beneficial effects of grapefruit IntegroPectin treatment in microglia cells exposed to oxidative stress conditions.Experimental procedureHuman microglial HMC3 cells were challenged with tert-butyl hydroperoxide (TBH), a powerful hydroperoxide, in the presence of grapefruit IntegroPectin. The apoptotic process, the oxidative stress and the neuroinflammatory responses with the relative intracellular cascades were evaluated.Key resultsGrapefruit IntegroPectin fully counteracted the apoptotic process induced by cell exposure to TBH. The protective effects of grapefruit IntegroPectin were accompanied with a decrease in the amount of ROS, and were strictly dependent on the activation of the PI3K/Akt cascade. Finally, IntegroPectin treatment inhibited basal microglia activation and the neuroinflammatory response by down-regulating the PI3K-NF-kB-iNOS cascade.Conclusions and implicationsThese findings reveal that the innovative IntegroPectin exerts a potent protective activity on microglia cells and strongly support further investigations aimed at exploring its therapeutic role inin vivomodels of neurodegenerative disorders.
Osteoporosis and its associated factors among patients attending Manakamana hospital, Chitwan
Authors: Dhakal Shankar , Sharma Kalpana , Adhikari Kishor , Joshi Alisha , Poudyal Sunita 
Published: July 2023

ABSTRACTBackgroundOsteoporosis is most common skeletal disorders that weaken bones and increase their susceptibility to fractures. It is becoming an urgent and serious global epidemic.Early diagnosis and treatment are essential to reduce morbidity and mortality associated with it. This study aimed to find out the prevalence of osteoporosis among patients attending at Manakamana Hospital, Bharatpur, Chitwan.MethodsA descriptive, cross-sectional study was adopted and 623 patients attending at orthopaedic outpatients department (OPD) of Manakamana Hospital were selected using consecutive sampling technique. Data were collected from 15thOctober 2021 to 15thApril, 2022, by using interview schedule, chart review and BMD measurement through calcaneal ultrasonography. Ethical approval was obtained from NHRC-IRC prior to study procedures. Obtained data were analysed using descriptive statistics and association between the variables were measured using chi-square test.ResultsThe mean age of the patients was 43.5 (±14.26) years. Nearly half (44%, n=274) were middle aged adults, 59.7% were female and 56.0% were involved in agriculture and household chores. Nearly half of the patients (45.7%) were overweight/ obese, 7.9% were smokers and 13.5% had habit of alcohol use. Osteopenia or low bone density was detected in 58.9% patients and 19.4% had osteoporosis. The prevalence of osteoporosis was significantly associated with age group (p=<0.001) and educational status (p=0.013) of the patients.ConclusionsOsteoporosis and osteopenia are prevalent in patients attending in the hospital. Hence, awareness, early screening, treatment is necessary for the hospital attended patients to minimize the risk of fracture and the consequences associated with it.
AmpliDiff: An Optimized Amplicon Sequencing Approach to Estimating Lineage Abundances in Viral Metagenomes
Authors: van Bemmelen Jasper , Smyth Davida S. , Baaijens Jasmijn A. 
Published: July 2023

AbstractMetagenomic profiling algorithms commonly rely on genomic differences between lineages, strains, or species to infer the relative abundances of sequences present in a sample. This observation plays an important role in the analysis of diverse microbial communities, where targeted sequencing of 16S and 18Sribosomal RNA(rRNA), both well-known hypervariable genomic regions, have led to insights into microbial diversity and the discovery of novel organisms. However, the variable nature of discriminatory regions can also act as a double-edged sword, as the sought-after variability can make it difficult to design primers for their amplification throughPolymerase Chain Reaction(PCR). Moreover, the most variable regions are not necessarily the most informative regions for the purpose of differentiation; one should focus on regions that maximize the number of lineages that can be distinguished. Here we present AmpliDiff, a computational tool that simultaneously finds such highly discriminatory genomic regions, as well as primers allowing for the amplification of these regions. We show that regions and primers found by AmpliDiff can be used to accurately estimate relative abundances of SARS-CoV-2 lineages, for example in wastewater sequencing data. We obtain mean absolute prediction errors that are comparable with using whole genome information to estimate relative abundances. Furthermore, our results show that AmpliDiff is robust against incomplete input data and that primers designed by AmpliDiff continue to bind to genomes originating from months after the primers were selected. With AmpliDiff we provide an effective and efficient alternative to whole genome sequencing for estimating lineage abundances in viral metagenomes.
Pre-University engagement and education for sustainable development in Aruba: The Academic Foundation Year
Authors: Chapman Mollie , Eppinga Maarten B. , de Scisciolo Tobia , Mijts Eric N. 
Published: July 2023

Purpose
Universities of Small Island States (SIS) have the potential to fulfill a crucial role in implementing the Sustainable Development Goals (SDGs) but also face barriers to local capacity building. The University of Aruba partly addresses these challenges through the development of The Academic Foundation Year (AFY), a one-year pre-university program aiming to optimally equip students for higher education. This study aims to assess to what extent the program can foster the local embeddedness of the students in ecology, culture and history and an understanding of opportunities and challenges for sustainable development in SIS.

Design/methodology/approach
The authors provide examples of how the program incorporates education for sustainable development and how it emphasizes experiential learning. In addition, quantitative survey data and qualitative analysis of focus group meetings are used to reflect on the program’s achievements and its potential for further development.

Findings
The survey results suggest that AFY courses not only increase knowledge but also change students’ perceptions regarding sustainability. Indeed, key impacts emerging from the student focus group related to both academic preparation and engagement with sustainability. Reflections by teachers emphasized the importance of experiential learning, an expansive view of the SDGs and preparing students as citizens.

Originality/value
This study highlights that the program could provide a starting point for the development of similar initiatives in other SIS, the common basis being the fostering of sustainability literacy and social adoption of the SDGs.
Speed of price adjustment toward market efficiency of Indian agricultural commodity market: a market microstructure analysis of market quality
Authors: Pullaykkodi Sreekha , Acharya Rajesh H. 
Published: July 2023

PurposeThis study examines the semi-strong market efficiency of the Indian agricultural commodity market in light of market reforms and policies. This study investigates whether the market reforms have boosted the speed of price adjustment and influenced the market quality.Design/methodology/approachThe study used the daily data of nine agricultural commodities. To precisely capture the effects of market microstructure changes, this study split the whole data into pre- and post-ban and pre- and post-reform eras. To ascertain the velocity of price adjustment, the authors used the ARMA (1,1) model, and the ADD VRatio was employed to identify the price movement on a specific day.FindingsThis study found that full incorporation of information happens sometimes. The authors noticed no gradual progress in the quickness of price adjustment. Since both methods suggested the same result for the period, the authors confirm that market microstructure changes do not enhance market quality.Research limitations/implicationsThis research has implications for academicians, policymakers and market players.Originality/valueThe paper has twofold novelty. First, this is a contemporary topic, and very few studies have been done in the Indian agriculture context. Second, the study has implications for policymakers and government because it highlights the effects of structural changes on market quality and market efficiency.
Employee ownership in Spain: worker cooperatives and sociedades laborales
Authors: Marcuello Carmen 
Published: July 2023

PurposeThe aim of this paper is to describe the main characteristics of het two most recognised models in Spain: worker cooperatives and worker-owned companies (Sociedades Laborales).Design/methodology/approachTo this end, a review will be carried out of the main factors from the regulatory and financial framework and the support structures of these organisations model.FindingsThe main findings are the constant decrease in the number of SSLL over the past ten years and a certain decrease in the number of worker cooperatives. Some of the reasons put forward for this decline are the lack of effective favourable tax treatment; the establishment of more favourable measures for capitalist companies; the change of the single payment of unemployment benefits; the lack of knowledge and training professionals and politicians.Originality/valueThe main contribution of this paper is to provide a recent analysis of the regulatory changes and developments in the field of worker cooperatives and worker-owned companies in Spain.
Social sustainability for whom? Developing an analytical approach through a tripartite collaboration
Authors: Sunnemark Fredrik , Gahnström Emil , Rudström Hedvig , Karlsson Erika , Assmo Per 
Published: July 2023

Purpose
Social sustainability is a concept frequently referred to in public debates concerning how to construct the governance of future societies. The interpretations of its meaning, however, are ambiguous, and practices often dubious. Confronting top-down technocratic governance structures, this paper aims to argue for for tripartite collaborations between residents, higher education institutions (HEIs) and local government, as an approach toward social sustainability that involves residents’ interests in local governance.

Design/methodology/approach
This study argues that a specific time-spatial method of analysis can benefit the co-creation of knowledge as it passes through the spectrum of resident–HEI–local government. It provides a way for resident perceptions to become structured knowledge that originates from the residents, effectively engendering a bottom-up governance structure.

Findings
This study shows how to include residents in policymaking and implementation processes as co-creators of knowledge, and thereby displays the possibility of examining knowledge and competence within municipal projects for social sustainability.

Originality/value
The model developed in this study can be used as a methodological instrument to analyze and expand resident participation in local social sustainability work. It thereby provides a toolbox for inclusive policymaking and strategies.
Work–family conflict and organizational outcomes: moderating effect of intrinsic motivation among women in health care sector of Pakistan
Authors: Jamshed Hira , Noor Sadaf , Ali Hafiz Yasir , Arshad Hafiz Muhammad , Asrar-ul-Haq Muhammad 
Published: July 2023

PurposeThis study analyses the organizational consequences of work–family conflict (WFC) among female nurses in health care sector. Moreover, this study focuses on the moderating effect of intrinsic motivation on the association between WFC dimensions with different organizational outcomes.Design/methodology/approachData are collected from 347 female nurses working in health care sector at Islamabad, Rawalpindi, Lahore, Multan and Bahawalpur regions of Pakistan, using random sampling technique. Regression analysis is used to test the hypotheses of this study.FindingsThe findings demonstrate that WFC conflict lowers job satisfaction, affective commitment and organizational citizenship behaviour. Contrary, WFC reduces job satisfaction, affective commitment and organizational citizenship behaviour and increases turnover intentions among female nurses. Moreover, intrinsic motivation moderates the association between WFC and certain organizational outcomes.Originality/valueThe study offers valuable insights for female nurses at health care sector about WFC and finally leads to theoretical contributions and practical implications for the healthcare sector of Pakistan.
Acetyl coenzyme A carboxylase modulates lipogenesis and sugar homeostasis in Blattella germanica
Authors: Pei Xiaojin , Bai Tiantian , Luo Yuan , Zhang Zhanfeng , Li Sheng , Fan Yongliang , Liu Tong‐Xian 
Published: July 2023

AbstractLipid and sugar homeostasis is critical for insect development and survival. In this study, we characterized an acetyl coenzyme A carboxylase gene in Blattella germanica (BgACC) that is involved in both lipogenesis and sugar homeostasis. We found that BgACC was dominantly expressed in the fat body and integument, and was significantly upregulated after molting. Knockdown of BgACC in 5th‐instar nymphs did not affect their normal molting to the next nymphal stage, but it caused a lethal phenotype during adult emergence. BgACC‐RNA interference (RNAi) significantly downregulated total free fatty acid (FFA) and triacylglycerol (TAG) levels, and also caused a significant decrease of cuticular hydrocarbons (CHCs). Repression of BgACC in adult females affected the development of oocytes and resulted in sterile females, but BgACC‐RNAi did not affect the reproductive ability of males. Interestingly, knockdown of BgACC also changed the expression of insulin‐like peptide genes (BgILPs), which mimicked a physiological state of high sugar uptake. In addition, BgACC was upregulated when B. germanica were fed on a high sucrose diet, and repression of BgACC upregulated the expression of the glycogen synthase gene (BgGlyS). Moreover, BgACC‐RNAi increased the circulating sugar levels and glycogen storage, and a longevity assay suggested that BgACC was important for the survival of B. germanica under conditions of high sucrose uptake. Our results confirm that BgACC is involved in multiple lipid biogenesis and sugar homeostasis processes, which further modulates insect reproduction and sugar tolerance. This study benefits our understanding of the crosstalk between lipid and sugar metabolism.