bluuebunny/crossref_metadata_2025_split huggingface dataset:
Beyond Alar Base Resection – Contouring of the Alar Rim and Base
Authors: Rohrich Rod J. , Novak Matthew , Chiodo Michael , Lisiecki Jeffrey , Savetsky Ira , Cason Roger 
Published: May 2023

Summary:
Alar base surgery is approached with hesitancy and is often avoided by many surgeons because of inexperience and a lack of understanding. However, with a thorough knowledge of the anatomy and dynamic nature of the lower third of the nose, alar base resection can achieve favorable and reproducible results. Beyond correcting alar flare, an appropriately diagnosed and performed alar base procedure serves to contour both the alar rim and the alar base. The following article presents a case series of 436 consecutive rhinoplasties from a single surgeon, 214 of which underwent alar base surgery. The outcomes demonstrate that the procedure is safe and yields desirable results without a single revision being required. As the third in a series of three articles on alar base surgery by the senior author, this article unifies the management of the alar base. An intuitive approach to the classification and management of alar flare and the implications of alar base surgery on contouring of the alar base and alar rim is presented.
Mind the Gap
Authors: Dushnicky Molly J. , Fine Claire , Cellucci Tania , Garner Stephanie , Heale Liane , Matsos Mark , Beattie Karen A. , Batthish Michelle 
Published: May 2023

Background
The transition from pediatric to adult care is a critical time when adolescent patients and their families face many challenges. This period can be associated with an increase in disease-related morbidity and mortality. The aim of our study is to identify gaps in transition-related care to help guide areas for improvement.

Methods
Patients (14–19 years) with juvenile idiopathic arthritis or systemic lupus erythematosus and one of their parents were recruited from the McMaster Rheumatology Transition Clinic. Both were asked to complete the Mind the Gap questionnaire, a validated tool to assess experience and satisfaction with transition care in a clinic setting. The questionnaire, addressing 3 important domains of care: management of the environment, provider characteristics, and process issues, was completed twice—once based on their current clinical experience and again based on their ideal clinical encounter. Positive scores suggest current care is less than ideal; negative scores suggest current care exceeds the ideal experience

Results
Most patients (n = 65, 68% female) had a diagnosis of juvenile idiopathic arthritis (87%). Patients identified mean gap scores between 0.2 and 0.3 for each domain of Mind the Gap, with female patients having higher gap scores compared with male patients. Parents (n = 51) identified gap scores between 0.0 and 0.3. Patients identified process issues as having the largest gap, whereas parents identified management of the environment as having the largest gap.

Conclusions
We identified several gaps in transition clinic care relative to what patients and parents identify as ideal. These can be used to improve the rheumatology transition care that is currently being provided.
Titratable partial aortic occlusion: Extending Zone I endovascular occlusion times
Authors: Gomez David , Naveed Asad , Rezende Joao , Dennis Bradley M. , Kundi Rishi , Benjamin Elizabeth , Lawless Ryan , Nguyen Jonathan , Duchesne Juan , Spalding Chance , Doris Stephanie , Van Skike Candice , Moore Ernest E. , Beckett Andrew 
Published: May 2023

BACKGROUND
Extending the time to definitive hemorrhage control in noncompressible torso hemorrhage (NCTH) is of particular importance in the battlefield where transfer times are prolonged and NCTH remains the leading cause of death. While resuscitative endovascular balloon occlusion of the aorta is widely practiced as an initial adjunct for the management of NCTH, concerns for ischemic complications after 30 minutes of compete aortic occlusion deters many from zone 1 deployment. We hypothesize that extended zone 1 occlusion times will be enabled by novel purpose-built devices that allow for titratable partial aortic occlusion.

METHODS
This is a cross-sectional analysis describing pREBOA-PRO zone 1 deployment characteristics at seven level 1 trauma centers in the United States and Canada (March 30, 2021, and June 30, 2022). To compare patterns of zone 1 aortic occlusion, the AORTA registry was used. Data were limited to adult patients who underwent successful occlusion in zone 1 (2013–2022).

RESULTS
One hundred twenty-two patients pREBOA-PRO patients were included. Most catheters were deployed in zone 1 (n = 89 [73%]) with a median zone 1 total occlusion time of 40 minutes (interquartile range, 25–74). A sequence of complete followed by partial occlusion was used in 42% (n = 37) of zone 1 occlusion patients; a median of 76% (interquartile range, 60–87%) of total occlusion time was partial occlusion in this group. As was seen in the prospectively collected data, longer median total occlusion times were observed in the titratable occlusion group in AORTA compared with the complete occlusion group.

CONCLUSION
Longer zone 1 aortic occlusion times seen with titratable aortic occlusion catheters appear to be driven by the feasibility of controlled partial occlusion. The ability to extend safe aortic occlusion times may have significant impact to combat casualty care where exsanguination from NCTH is the leading source of potentially preventable deaths.

LEVEL OF EVIDENCE
Therapeutic/Care Management; Level IV.
Extracellular Vesicle–associated GARP/TGFβ:LAP Mediates “Infectious” Allo-tolerance
Authors: Burlingham William J. , Jankowska-Gan Ewa , Fechner John H. , Little Christopher J. , Wang Jianxin , Hong Seungpyo , Molla Miraf , Sullivan Jeremy A. , Foley David P. 
Published: May 2023

Background.
Here we test the hypothesis that, like CD81-associated “latent” IL35, the transforming growth factor (TGF)β:latency-associated peptide (LAP)/glycoprotein A repetitions predominant (GARP) complex was also tethered to small extracellular vesicles (sEVs), aka exosomes, produced by lymphocytes from allo-tolerized mice. Once these sEVs are taken up by conventional T cells, we also test whether TGFβ could be activated suppressing the local immune response.

Methods.
C57BL/6 mice were tolerized by i.p. injection of CBA/J splenocytes followed by anti-CD40L/CD154 antibody treatment on days 0, 2, and 4. On day 35, spleen and lymph nodes were extracted and isolated lymphocytes were restimulated with sonicates of CBA splenocytes overnight. sEVs were extracted from culture supernatants by ultracentrifugation (100 000g) and assayed for (a) the presence of TGFβ:LAP associated with tetraspanins CD81,CD63, and CD9 by enzyme-linked immunosorbent assay; (b) GARP, critical to membrane association of TGFβ:LAP and to activation from its latent form, as well as various TGFβ receptors; and (c) TGFβ-dependent function in 1° and 2° immunosuppression of tetanus toxoid-immunized B6 splenocytes using trans-vivo delayed–type hypersensitivity assay.

Results.
After tolerization, CBA-restimulated lymphocytes secreted GARP/TGFβ:LAP-coated extracellular vesicles. Like IL35 subunits, but unlike IL10, which was absent from ultracentrifuge pellets, GARP/TGFβ:LAP was mainly associated with CD81+ exosomes. sEV-bound GARP/TGFβ:LAP became active in both 1° and 2° immunosuppression, the latter requiring sEV uptake by “bystander” T cells and reexpression on the cell surface.

Conclusions.
Like other immune-suppressive components of the Treg exosome, which are produced in a latent form, exosomal GARP/TGFβ:LAP produced by allo-specific regulatory T cells undergoes either immediate activation (1° suppression) or internalization by naive T cells, followed by surface reexpression and subsequent activation (2°), to become suppressive. Our results imply a membrane-associated form of TGFβ:LAP that, like exosomal IL35, can target “bystander” lymphocytes. This new finding implicates exosomal TGFβ:LAP along with Treg-derived GARP as part of the infectious tolerance network.
Adjuvant Chemotherapy and Effect on Long-Term Survival in Ampullary Adenocarcinoma: A Multicenter Cohort Study
Authors: Shin Dong Woo , Lee Jae Min , Lee Jong-chan , Lee Hee Seung , Yoon Seung Bae , Jang Dong Kee , Park Joo Kyung , Jung Min Kyu , Lee Yoon Suk , Hwang Jin-Hyeok 
Published: May 2023

BACKGROUND:
The role of adjuvant chemotherapy (AC) in patients with ampullary adenocarcinoma (AA) remains controversial. This study aimed to determine if AC could improve the prognosis of patients with resected AA.

STUDY DESIGN:
This study enrolled patients diagnosed with AA at 9 tertiary teaching hospitals. Patients who did and did not receive AC were matched 1:1 using propensity score. The overall survival (OS) and recurrence-free survival (RFS) were compared between the 2 groups.

RESULTS:
Of the 1,057 patients with AA, 883 underwent curative-intent pancreaticoduodenectomy, and 255 received AC. Because patients with advanced-stage AA received AC more frequently, the no AC group unexpectedly had a longer OS (not reached vs 78.6 months; p < 0.001) and RFS (not reached vs 18.7 months; p < 0.001) than did the AC group in the unmatched cohort. In the propensity score–matched cohort (n = 296), no difference between the 2 groups in terms of OS (95.9 vs 89.8 months, p = 0.303) and RFS (not reached vs 25.5 months; p = 0.069) was found. By subgroup analysis, patients with advanced stage (pT4 or pN1-2) showed longer OS in the AC group than in the no AC group (not reached vs 15.7 months, p = 0.007: 89.8 vs 24.2 months, p = 0.006, respectively). There was no difference in RFS according to AC in the propensity score–matched cohort.

CONCLUSIONS:
Given its favorable long-term outcomes, AC can be recommended for patients with resected AA, especially those in the advanced stage (pT4 or pN1-2).
Topological effects of a global monopole on a spherical quantum dot
Authors: Bakke Knut 
Published: May 2023

We consider a spherical quantum dot described by an attractive short-range potential and search for topological effects of a global monopole on it. The attractive short-range potential is built from the power-exponential potential model in Ciurla
et al.
(2022
Physica E
15
, 261–268 (
doi:10.1016/S1386-9477(02)00572-6
)), where we focus on a particular case proposed by this quantum dot model. In search of bound states, we deal with

s

-waves. We thus obtain the energy eigenvalues and discuss the effects of the topology of the global monopole on them. Further, we extend our discussion to the quantum revivals.
Elastic wave dispersion in layered media with suture joints: influence of structural hierarchy and viscoelasticity
Authors: Ongaro Federica , Bosia Federico , Pugno Nicola M. 
Published: May 2023

Suture joints contribute to the exceptional combination of stiffness, strength, toughness and efficient load bearing and transmission of many biological structures like the cranium or ammonite fossil shells. However, their role in the attenuation of vibrations and effect on dynamic loads is less clear. Moreover, the self-similar hierarchical geometry often associated with suture joints renders its treatment with standard numerical approaches computationally prohibitive. To address this problem, this paper investigates the dynamic response of periodic layered media with suture joints using an analytical approach based on material homogenization. A general trapezoidal suture geometry is considered together with the fundamental ingredients of hierarchy and viscoelasticity. The Spectral Element Method and Bloch theorem are used to derive the dispersion relation and band diagram of the system, including propagating and evanescent dispersion modes. A strong influence of the suture morphology and material properties emerges, and the analysis reveals an important advantage of adding hierarchy, i.e. the possibility of simultaneously obtaining wider bandgaps and their shift to higher frequencies. A synergy between hierarchy and structure is also observed, providing superior levels of wave attenuation. These findings suggest a possible design concept for bioinspired devices with efficient and tailorable wave attenuation properties.
Massilia agrisoli sp. nov., isolated from rhizospheric soil of banana
Authors: Huq Md. Amdadul , Ma Juncai , Srinivasan Sathiyaraj , Parvez Md. Anowar Khasru , Rahman M. Mizanur , Naserkheil Masoumeh , Abuhena Md. , Maitra Pulak , Islam Fahadul , NAM Kihong , Park Jong-Hyun , Akter Shahina 
Published: May 2023

A Gram-stain-negative, aerobic, short rod-shaped and motile novel bacterial strain, designated MAHUQ-52T, was isolated from the rhizospheric soil of a banana plant. Colonies grew at 10–35 °C (optimum, 28 °C), pH 6.0–9.5 (optimum, pH 7.0–7.5), and in the presence of 0–1.0 % NaCl (optimum 0 %). The strain was positive for catalase and oxidase tests, as well as hydrolysis of gelatin, casein, starch and Tween 20. Based on the results of phylogenetic analysis using 16S rRNA gene and genome sequences, strain MAHUQ-52T clustered together within the genus

Massilia

. Strain MAHUQ-52T was closely related to

Massilia soli

R798T (98.6 %) and

Massilia polaris

RP-1-19T (98.3 %). The novel strain MAHUQ-52T has a draft genome size of 4 677 454 bp (25 contigs), annotated with 4193 protein-coding genes, 64 tRNA and 19 rRNA genes. The genomic DNA G+C content was 63.0 %. The average nucleotide identity (ANI) and digital DNA–DNA hybridization (dDDH) values between strain MAHUQ-52T and closely related type strains were ≤88.4 and 35.8 %, respectively. The only respiratory quinone was ubiquinone-8. The major fatty acids were identified as C16 : 0 and summed feature 3 (C15 : 0 iso 2-OH and/or C16 : 1
ω7c). Strain MAHUQ-52T contained phosphatidylethanolamine, diphosphatidylglycerol and phosphatidylglycerol as the major polar lipids. On the basis of dDDH and ANI values, as well as genotypic, chemotaxonomic and physiological data, strain MAHUQ-52T represents a novel species within the genus

Massilia

, for which the name Massilia agrisoli sp. nov. is proposed, with MAHUQ-52T (=KACC 21999T=CGMCC 1.18577T) as the type strain.
Stakelama marina sp. nov., isolated from seawater of the Tangyin hydrothermal field in the Okinawa Trough
Authors: Wang Shuang , Cao Xiaochen , Liu Lijun , Liu Ronghua , Bian Zeran , Zhang Xiao-Hua , Yu Min 
Published: May 2023

A Gram-stain-negative, strictly aerobic, rod-shaped and non-flagellated marine bacterium, designated strain LXI357T, was isolated from deep-sea water sampled at the Tangyin hydrothermal field in the Okinawa Trough. The growth temperature range was 20–45 °C (optimum, 28 °C). Strain LXI357T was also able to grow at pH 5.0–7.5 (optimum, pH 6.0–7.0) and in the presence of 0.5–11 % (optimum, 7%, w/v) NaCl. Strain LXI357T was oxidase-negative and catalase-positive. The predominant fatty acids were C18 : 1
ω7c and C16 : 0. The major polar lipids of strain LXI357T contained phosphatidylethanolamine, phosphatidylglycerol, phosphatidylcholine, phospholipid, sphingoglycolipid, diphosphatidylglycero and an unidentified aminolipid. Based on the results of 16S rRNA gene sequence analysis, strain LXI357T belonged to the genus

Stakelama

and was most closely related to

Stakelama flava

CBK3Z-3T (96.28%, 16S rRNA gene sequence similarity), followed by

Stakelama algicida

Yeonmyeong 1-13T (95.67%),

Stakelama pacifica

JLT832T (95.46%) and

Sphingosinicella vermicomposti

YC7378T (95.43%). Genome relatedness between strain LXI357T and

Stakelama flava

CBK3Z-3T was computed using average nucleotide identity, digital DNA–DNA hybridization and average amino acid identity, with values of 76.02, 20.9 and 71.1 %, respectively. The genomic DNA G+C content of strain LXI357T is 64.1 mol%. In addition, strain LXI357T has multiple genes related to sulphur metabolism, including genes encoding for the Sox system. The morphological, physiological, chemotaxonomic and phylogenetic analyses clearly distinguished strain LXI357T from its closest phylogenetic neighbours. According to the results of polyphasic analyses, strain LXI357T is considered to represent a novel species of the genus

Stakelama

, for which the name Stakelama marina sp. nov. is proposed. The type strain is LXI357T (=MCCC 1K06076T=KCTC 82726T).
H2A.Z histone variants facilitate HDACi-dependent removal of H3.3K27M mutant protein in paediatric high-grade glioma cells
Authors: Leszczynska Katarzyna B. , Pereira de Freitas Amanda , Jayaprakash Chinchu , Dzwigonska Monika , Wojnicki Kamil , Gielniewski Bartlomiej , Szadkowska Paulina , Kaza Beata , Ciolkowski Maciej K. , Trubicka Joanna , Grajkowska Wieslawa , Kaminska Bozena , Mieczkowski Jakub 
Published: May 2023

ABSTRACTDiffuse intrinsic pontine gliomas (DIPG) are deadly paediatric brain tumours, non-resectable due to brainstem localisation and diffusive growth. Patients with DIPG have a dismal prognosis of 9-12 months of survival with no effective therapy. Over 80% of DIPGs harbour a mutation in histone 3 (H3.3 or H3.1) resulting in a lysine to methionine substitution (H3K27M). H3K27M causes global epigenetic alterations (a loss of H3K27 trimethylation and an increase in H3K27 acetylation) resulting in aberrant gene expression. To date, no therapeutic strategy exists to suppress the levels of oncogenic H3K27M.We show that pan-HDAC inhibitors (HDACi) lead to the temporary but significant reduction in the H3.3K27M protein (up to 80%) in multiple glioma cell lines expressing the H3.3K27M histone variant, without changes in theH3F3AmRNA expression. The H3.3K27M occupancy at the chromatin is greatly reduced upon HDACi (SB939) treatment, as shown by ChIPseq analysis. H3.3K27M loss is most striking at SB939-upregulated genes suggesting the role in repression of these genes. In addition, genes previously reported as H3K27M-dependent become downregulated in response to SB939 treatment. We discover that the SB939-mediated loss of H3.3K27M is partially blocked by a lysosomal inhibitor, chloroquine. Moreover, the loss of H3.3K27M is facilitated by co-occurrence of H2A.Z, as evidenced by the knock-down of H2A.Z histone isoforms. ChIPseq analysis confirms the occupancy of H3.3K27M and H2A.Z at the same SB939-inducible genes.Altogether, we provide new insight into disease-specific mechanism of HDAC inhibition and demonstrate pharmacological modulation of the oncogenic H3.3K27M protein levels. These findings open a new possibility to directly target the H3.3K27M oncohistone, which may be exploited in future therapies.
Distinct T cell functional profiles in SARS-CoV-2 seropositive and seronegative children associated with endemic human coronavirus cross-reactivity
Authors: Benede Ntombi S. B. , Tincho Marius B. , Walters Avril , Subbiah Vennesa , Ngomti Amkele , Baguma Richard , Butters Claire , Mennen Mathilda , Skelem Sango , Adriaanse Marguerite , van Graan Strauss , Balla Sashkia R. , Moyo-Gwete Thandeka , Moore Penny L. , Botha Maresa , Workman Lesley , Zar Heather J. , Ntusi Ntobeko A. B. , Zühlke Liesl , Webb Kate , Riou Catherine , Burgers Wendy A. , Keeton Roanne S. 
Published: May 2023

SUMMARYSARS-CoV-2 infection in children typically results in asymptomatic or mild disease. There is a paucity of studies on antiviral immunity in African children. We investigated SARS-CoV-2-specific T cell responses in 71 unvaccinated asymptomatic South African children who were seropositive or seronegative for SARS-CoV-2. SARS-CoV-2-specific CD4+ T cell responses were detectable in 83% of seropositive and 60% of seronegative children. Although the magnitude of the CD4+ T cell response did not differ significantly between the two groups, their functional profiles were distinct, with SARS-CoV-2 seropositive children exhibiting a higher proportion of polyfunctional T cells compared to their seronegative counterparts. The frequency of SARS-CoV-2-specific CD4+ T cells in seronegative children was associated with the endemic human coronavirus (HCoV) HKU1 IgG response. Overall, the presence of SARS-CoV-2-responding T cells in seronegative children may result from cross-reactivity to endemic coronaviruses and could contribute to the relative protection from disease observed in SARS-CoV-2-infected children.
Neuroactive metabolites and bile acids are altered in extremely premature infants with brain injury
Authors: Pristner Manuel , Wasinger Daniel , Seki David , Klebermaß-Schrehof Katrin , Berger Angelika , Berry David , Wisgrill Lukas , Warth Benedikt 
Published: May 2023

AbstractThe gut microbiome has been associated with pathological neurophysiological evolvement in extremely premature infants suffering from brain injury. The exact underlying mechanism and its associated metabolic signatures in infants are not fully understood. To decipher metabolite profiles linked to neonatal brain injury, we investigated the longitudinal fecal and plasma metabolome of 51 extremely premature infants using LC-HRMS-based untargeted metabolomics. This was expanded by an investigation of bile acids and amidated bile acid conjugates in feces and plasma by LC-MS/MS-based targeted metabolomics. The resulting data was integrated with 16S rRNA gene amplicon gut microbiome profiles as well as patient cytokine, growth factor and T-cell profiles. We identified an early onset of differentiation in neuroactive metabolites and bile acids between infants with and without brain injury. We detected several bacterially-derived bile acid amino acid conjugates and secondary bile acids in the plasma already three days after delivery, indicating the early establishment of a metabolically active gut microbiome. These results give new insights into the early life metabolome of extremely premature infants.
Symptom onset and cellular pathology in facioscapulohumeral muscular dystrophy is accelerated by cigarette smoking
Authors: Banerji Christopher R. S. , Heher Philipp , Hogan John , Katz Natalie , Haidar Husain Bin , Keegan Michael D. , Cernik Colin , Tawil Rabi , Patel Ketan , Zammit Peter S. , Statland Jeffery M. 
Published: May 2023

AbstractFacioscapulohumeral muscular dystrophy (FSHD) is an incurable skeletal myopathy. In absence of therapy, lifestyle factors impacting disease progression are important for clinical management. Monozygotic twins with FSHD often exhibit dramatically different disease progression, indicating existence of environmental disease modifiers. Here we analyse the USA National Registry for Myotonic Dystrophy & Facioscapulohumeral Dystrophy, comprising 511 FSHD1 patients followed up annually for an average of 8 years. This multimodal, longitudinal dataset comprises 189 baseline and 37 annually assessed features. We developed a workflow for prospective cohort analysis and identify cigarette smoking as associated with a two-fold increase in risk of facial and lower limb involvement in FSHD1 patients. Our definition of lower limb involvement includes inability to run and climb steps unaided, important functional outcomes for FSHD patients. We then employed an assay to test the effects of cigarette smoke extract on human myoblasts in vitro. Cigarette smoke extract drove disproportionate defects in proliferation and myogenic differentiation of FSHD1 patient-derived myoblasts, compared to matched controls. Mitochondrial function was also inordinately affected in FSHD1 myoblasts exposed to cigarette smoke extract, with increased mitochondrial membrane potential and mitochondrial radical oxygen species (mitoROS) generation. Our findings support recommending smoking cessation in clinical management of FSHD.