bluuebunny/crossref_metadata_2025_split huggingface dataset:
Seismic robustness computational methodology of community building portfolio coupled with water supply network based on probability‐cloud model
Authors: Zhang Juan , Zhang Mingyuan 
Published: September 2023

AbstractA novel seismic robustness computational methodology is proposed for community building portfolio (CBP) coupled with lifeline networks based on probability‐cloud model. This method consists of three interrelated submodels, respectively, the building fragility analysis model of the main subsystem CPB, the water supply network (WSN) hydraulic simulation analysis model of the secondary subsystem WSN, and the probability‐cloud model. The proposed method can be used to predict the seismic robustness of hierarchical systems. The computational methodology uses a joint probabilistic model combined with building fragility analysis and WSN hydraulic simulation analysis to evaluate the robustness of CPB coupled with WSN. Due to the uncertainty in robustness analysis, a probabilistic‐cloud model procedure considering the uncertainty in seismic robustness is proposed. The procedure enables a qualitative and quantitative integrated analysis of the robustness of CBP coupled with WSN. Finally, a community with 1406 buildings in China is simulated to illustrate the method. The research outcomes can provide a useful reference for the seismic robustness analysis of CBP coupled with WSN.
Freedom and Necessity of the Creative Act: The Cosmological Aspect of Kandinsky's Principle of Inner Necessity
Authors: Moulin Isabelle 
Published: September 2023

AbstractThis article argues that freedom in the divine creative act is better understood as a freedom of consent rather than freedom of will. However, even if some conundrums are thus avoided, one has to face the apparent antinomy between both God's creative act from His very nature and God's absolute freedom. Freedom of consent, as defined in David Burrell's works, finds a resonance with the French spiritualist philosopher, Henri Bergson, who resolves the apparent antinomy by showing that the necessity of the action springs from the nature of the self in a way that does not contradict the free perpetual auto‐creation of the consciousness. Notwithstanding its defects, the artisan model found in Genesis allows one to draw a parallelism between the divine creative act and artistic creation. Kandinsky faced precisely such an antinomy, for his principle of inner necessity combines both the absolute free creative power of the artist and the necessity emerging from the different levels of the cosmic order. To exemplify Kandinsky's solution, this article offers an original reading of Painting with White Border, based on Kandinsky's analysis in Reminiscences.
The genetic and physiological basis of Arabidopsis thaliana tolerance to Pseudomonas viridiflava
Authors: Duque‐Jaramillo Alejandra , Ulmer Nina , Alseekh Saleh , Bezrukov Ilja , Fernie Alisdair R. , Skirycz Aleksandra , Karasov Talia L. , Weigel Detlef 
Published: September 2023

Summary

The opportunistic pathogen Pseudomonas viridiflava colonizes > 50 agricultural crop species and is the most common Pseudomonas in the phyllosphere of European Arabidopsis thaliana populations. Belonging to the P. syringae complex, it is genetically and phenotypically distinct from well‐characterized P. syringae sensu stricto. Despite its prevalence, we lack knowledge of how A. thaliana responds to its native isolates at the molecular level. Here, we characterize the host response in an A. thaliana – P. viridiflava pathosystem.

We measured host and pathogen growth in axenic infections and used immune mutants, transcriptomics, and metabolomics to determine defense pathways influencing susceptibility to P. viridiflava infection.

Infection with P. viridiflava increased jasmonic acid (JA) levels and the expression of ethylene defense pathway marker genes. The immune response in a susceptible host accession was delayed compared with a tolerant one. Mechanical injury rescued susceptibility, consistent with an involvement of JA.

The JA/ethylene pathway is important for suppression of P. viridiflava, yet suppression capacity varies between accessions. Our results shed light on how A. thaliana can suppress the ever‐present P. viridiflava, but further studies are needed to understand how P. viridiflava evades this suppression to spread broadly across A. thaliana populations.
Depletion of TIAR impairs embryogenesis via inhibiting zygote genome transcribe
Authors: Liu Ke , Dai Weilong , Ju Jiaqian , Li Weijian , Chen Qianqian , Li Juan , Liu Honglin 
Published: September 2023

AbstractT cell intracellular antigen 1 related protein (TIAR), an RNA‐binding protein (RBP), regulates pre‐messenger RNA (pre‐mRNA) alternative splicing, has been suggested to affect the maturation of primordial germ cells and early mouse embryo development. However, the underlying mechanism remains elusive. In this study, we revealed that TIAR was primarily located in the nucleus at the 2‐cell stage embryo, accompanied by highly active transcription. Using immunofluorescence staining and western blotting, we first described the localization and expression level of TIAR during the whole period of oocyte matured and embryogenesis. Knocked down of TIAR could significantly inhibit transcribed and blocked the early mouse embryo development. Combined with RNAP II inhibitor and pre‐RNA splicing inhibitor treatment, we further supposed that TIAR might affect transcription at 2‐cell via regulating pre‐mRNA splicing, and then regulate early mouse embryo development. Collectively, our results provided a novel and potential understanding of TIAR in embryogenesis, suggesting TIAR is required for transcription and embryonic development.
Association of MTNR1A and GDF9 gene allelles with the reproductive performance, response to oestrus induction treatments and prolificacy, in improved and non‐improved local indigenous sheep breeds
Authors: Antonopoulou Danai , Giantsis Ioannis A. , Symeon George K. , Avdi Melpomeni 
Published: September 2023

AbstractSheep farming plays a crucial role for Mediterranean countries, wherefrom a plethora of dairy products is produced. Yet, numerous indigenous sheep breeds in temperate latitudes such as the Mediterranean present a serious drawback that milk production is impaired by the seasonality of reproduction. Towards the efforts for reduction of this phenomenon, the purpose of this study was to evaluate and compare different oestrus synchronization treatments, as well as to associate two genes alleles', namely the MTNR1A and the GDF9, with the response to those treatments. Three indigenous breeds were investigated (Florina, Chios and Karagouniko sheep) and inside each breed three different oestrus synchronization treatments were applied (A: intravaginal sponges, B: GNRH use and C: male effect). In group A, Florina ewes expressed oestrus at 90% in July and fecundity was 85%. Karagouniko and Chios ewes exhibited an oestrus expression of 100% with fecundity rates at 95% and 99%, respectively. In group B, Florina ewes expressed oestrus at 60% with fecundity at 57%, Karagouniko ewes expressed oestrus at 65% with fecundity at 54%, whereas Chios breed animals expressed oestrus at 87% with fecundity rate at 85%. In group C, 68% of the Florina breed expressed oestrus 20–25 days post ram induction, whereas this proportion was 84% and 94%, for Karagouniko and Chios breed, respectively. For the molecular analysis, partial segments of the two genes were sequenced and analysed, whereas alleles were scored based on the detected SNPs. All frequencies of the four detected SNPs in MTNR1A gene were statistically and significantly different in ewes that expressed oestrus in comparison with ewes that did not express oestrus in Florina and Karagouniko breeds concerning all treatments. Two SNP's were detected in GDF9 gene, G1 and FecG, from which, only the FecG mutation exhibited statistically significant difference in twins and triplets than in singles in Florina and Karagouniko breeds.
Big five personality and COVID‐19 beliefs, behaviors, and vaccine intentions: The mediating role of political ideology
Authors: Panish Adam R. , Ludeke Steven G. , Vitriol Joseph A. 
Published: September 2023

AbstractBecause personal health decisions can impact the health of the broader community, researchers have increasingly sought to understand the psychological bases for different responses to public health communications and prescriptions. We contribute to this literature in two ways. First, we analyze the relationship between Big Five personality traits and three critical beliefs and behaviors in the context of the COVID‐19 pandemic. These are vaccine hesitancy, engagement in risky (vs. self‐protective) pandemic social behaviors, and conspiratorial beliefs surrounding the origins, consequences, and public health response to COVID‐19. Second, we draw on theory from political psychology to model the joint effects of personality and ideology. Our analysis of two American samples (MTurk = 510; Representative = 441) indicated that political liberalism mediated the relationship between Openness and COVID‐related attitudes and behaviors.
Treatment of a nasal angiofibroma in a cat using stereotactic body radiation therapy
Authors: Upton Laurel , Lee Nathan , Rechy Jaime , Lucas Pamela W. 
Published: September 2023

AbstractA 14‐year‐old cat presented with right‐sided epistaxis, right facial swelling, hyporexia, and sneezing. A right nasal mass was diagnosed based on dental radiography and computed tomography (CT), and nasal angiofibroma was diagnosed based on histopathology. Treatment consisted of stereotactic body radiation therapy in three consecutive daily doses. Self‐limiting grade 3 oral mucositis developed which resolved within 6 weeks. Recheck CT 169 days after treatment confirmed a partial response by RECIST(1) based on digital CT measurements . Disease progression was confirmed on CT 642 days after treatment, per RECIST criteria, with the longest tumor diameter measuring 3.4 cm.
Computed tomographic imaging of a maxillary sinus mucocele in an 8‐year‐old French Bulldog
Authors: Baeza Mercedes , Young Benjamin D. , LaQuaglia Kathryn A. , Rollins Amanda , Aboellail Tawfik A. 
Published: September 2023

AbstractAn 8‐year‐old male neutered French Bulldog was referred for continued nasal dyspnea following a staphylectomy revision performed one month prior to presentation. The patient had a prior history of skin allergies and underwent brachycephalic airway surgery performed at one year of age. Computed tomography (CT) revealed an osseous‐encased, cystic mass arising from the right maxillary sinus. Surgical biopsies were performed and a mucocele with sinusitis and glandular hyperplasia was diagnosed. Based on our systematic review of the literature, maxillary sinus mucocele has not been reported in the dog and should be among the differentials for sinus cystic masses.
Ultrasonographic diagnosis of a lymphomatous lymph node presumptively infiltrating the ureter causing hydronephrosis in a 3‐year‐old dog
Authors: Dempsey Kayla , Moon Rachel , Holland Merrilee , Cole Robert , Fiske Kaitlin , Schettler Michael 
Published: September 2023

AbstractA 3‐year‐old male neutered mixed breed dog was presented for chronic vomiting and diarrhea. Abdominal ultrasound revealed a large amorphous, heterogeneous mass within the left mid to caudal abdomen most consistent with medial iliac lymph node. It appeared to invade the left ureter and extend distally causing ureteral obstruction and hydronephrosis. Concurrent additional ultrasound findings were consistent with metastatic or multicentric neoplasia. Fine needle aspirates of the lymph node and spleen both confirmed large cell lymphoma. These findings present evidence of lymphoma invading directly from an organ into the ureter which has not previously been reported in dogs.
Enhanced mitochondrial buffering prevents Ca2+ overload in naked mole‐rat brain
Authors: Cheng Hang , Perkins Guy A. , Ju Saeyeon , Kim Keunyoung , Ellisman Mark H. , Pamenter Matthew E. 
Published: September 2023

AbstractDeleterious Ca2+ accumulation is central to hypoxic cell death in the brain of most mammals. Conversely, hypoxia‐mediated increases in cytosolic Ca2+ are retarded in hypoxia‐tolerant naked mole‐rat brain. We hypothesized that naked mole‐rat brain mitochondria have an enhanced capacity to buffer exogenous Ca2+ and examined Ca2+ handling in naked mole‐rat cortical tissue. We report that naked mole‐rat brain mitochondria buffer >2‐fold more exogenous Ca2+ than mouse brain mitochondria, and that the half‐maximal inhibitory concentration (IC50) at which Ca2+ inhibits aerobic oxidative phosphorylation is >2‐fold higher in naked mole‐rat brain. The primary driving force of Ca2+ uptake is the mitochondrial membrane potential (Δψm), and the IC50 at which Ca2+ decreases Δψm is ∼4‐fold higher in naked mole‐rat than mouse brain. The ability of naked mole‐rat brain mitochondria to safely retain large volumes of Ca2+ may be due to ultrastructural differences that support the uptake and physical storage of Ca2+ in mitochondria. Specifically, and relative to mouse brain, naked mole‐rat brain mitochondria are larger and have higher crista density and increased physical interactions between adjacent mitochondrial membranes, all of which are associated with improved energetic homeostasis and Ca2+ management. We propose that excessive Ca2+ influx into naked mole‐rat brain is buffered by physical storage in large mitochondria, which would reduce deleterious Ca2+ overload and may thus contribute to the hypoxia and ischaemia‐tolerance of naked mole‐rat brain.
imageKey points
Unregulated Ca2+ influx is a hallmark of hypoxic brain death; however, hypoxia‐mediated Ca2+ influx into naked mole‐rat brain is markedly reduced relative to mice.
This is important because naked mole‐rat brain is robustly tolerant against in vitro hypoxia, and because Ca2+ is a key driver of hypoxic cell death in brain.
We show that in hypoxic naked mole‐rat brain, oxidative capacity and mitochondrial membrane integrity are better preserved following exogenous Ca2+ stress.
This is due to mitochondrial buffering of exogenous Ca2+ and is driven by a mitochondrial membrane potential‐dependant mechanism.
The unique ultrastructure of naked mole‐rat brain mitochondria, as a large physical storage space, may support increased Ca2+ buffering and thus hypoxia‐tolerance.
Glutamine synthetase and GlnR regulate nitrogen metabolism in
Paenibacillus polymyxa
WLY78
Authors: Zhao Xiyun , Song Yi , Wang Tianshu , Hua Chongchong , Hu Rui , Shang Yimin , Shi Haowen , Chen Sanfeng 
Published: September 2023

ABSTRACT

Paenibacillus polymyxa
WLY78, a N
2
-fixing bacterium, has great potential use as a biofertilizer in agriculture. Recently, we have revealed that GlnR positively and negatively regulates the transcription of the
nif
(
ni
trogen
f
ixation) operon (
nifBHDKENXhesAnifV
) in
P. polymyxa
WLY78 by binding to two loci of the
nif
promoter according to nitrogen availability. However, the regulatory mechanisms of nitrogen metabolism mediated by GlnR in the
Paenibacillus
genus remain unclear. In this study, we have revealed that glutamine synthetase (GS) and GlnR in
P. polymyxa
WLY78 play a key role in the regulation of nitrogen metabolism.
P. polymyxa
GS (encoded by
glnA
within
glnRA
) and GS1 (encoded by
glnA1
) belong to distinct groups: GSI-α and GSI-β. Both GS and GS1 have the enzyme activity to convert NH
4
+
and glutamate into glutamine, but only GS is involved in the repression by GlnR. GlnR represses transcription of
glnRA
under excess nitrogen, while it activates the expression of
glnA1
under nitrogen limitation. GlnR simultaneously activates and represses the expression of
amtBglnK
and
gcvH
in response to nitrogen availability. Also, GlnR regulates the expression of
nasA, nasD1D2, nasT, glnQHMP,
and
glnS
.

IMPORTANCE

In this study, we have revealed that
Paenibacillus polymyxa
GlnR uses multiple mechanisms to regulate nitrogen metabolism. GlnR activates or represses or simultaneously activates and inhibits the transcription of nitrogen metabolism genes in response to nitrogen availability. The multiple regulation mechanisms employed by
P. polymyxa
GlnR are very different from
Bacillus subtilis
GlnR which represses nitrogen metabolism under excess nitrogen. Both GS encoded by
glnA
within the
glnRA
operon and GS1 encoded by
glnA1
in
P. polymyxa
WLY78 are involved in ammonium assimilation, but only GS is required for regulating GlnR activity. The work not only provides significant insight into understanding the interplay of GlnR and GS in nitrogen metabolism but also provides guidance for improving nitrogen fixation efficiency by modulating nitrogen metabolism.
Transcriptional regulation of soluble methane monooxygenase via enhancer-binding protein derived from
Methylosinus sporium
5
Authors: Hwang Yunha , Na Jeong-Geol , Lee Seung Jae 
Published: September 2023

ABSTRACT

Methane is a major greenhouse gas, and methanotrophs regulate the methane level in the carbon cycle. Soluble methane monooxygenase (sMMO) is expressed in various methanotroph genera, including
Alphaproteobacteria
and
Gammaproteobacteria
, and catalyzes the hydroxylation of methane to methanol. It has been proposed that MmoR regulates the expression of sMMO as an enhancer-binding protein under copper-limited conditions; however, details on this transcriptional regulation remain limited. Herein, we elucidate the transcriptional pathway of sMMO depending on copper ion concentration, which affects the interaction of MmoR and sigma factor. MmoR and sigma-54 (σ
54
) from
Methylosinus sporium
5 were successfully overexpressed in
Escherichia coli
and purified to investigate sMMO transcription in methanotrophs. The results indicated that σ
54
binds to a promoter positioned −24 (GG) and −12 (TGC) upstream between
mmoG
and
mmoX1
. The binding affinity and selectivity are lower (
K

d

= 184.6 ± 6.2 nM) than those of MmoR. MmoR interacts with the upstream activator sequence (UAS) with a strong binding affinity (
K

d

= 12.5 ± 0.5 nM). Mutational studies demonstrated that MmoR has high selectivity to its binding partner (ACA-xx-TGT). Titration assays have demonstrated that MmoR does not coordinate with copper ions directly; however, its binding affinity to UAS decreases in a low-copper-containing medium. MmoR strongly interacts with adenosine triphosphate (
K

d

= 62.8 ± 0.5 nM) to generate RNA polymerase complex. This study demonstrated that the binding events of both MmoR and σ
54
that regulate transcription in
M. sporium
5 depend on the copper ion concentration.

IMPORTANCE

This study provides biochemical evidence of transcriptional regulation of soluble methane monooxygenase (sMMO) in methanotrophs that control methane levels in ecological systems. Previous studies have proposed transcriptional regulation of MMOs, including sMMO and pMMO, while we provide further evidence to elucidate its mechanism using a purified enhancer-binding protein (MmoR) and transcription factor (σ
54
). The characterization studies of σ
54
and MmoR identified the promoter binding sites and enhancer-binding sequences essential for sMMO expression. Our findings also demonstrate that MmoR functions as a trigger for sMMO expression due to the high specificity and selectivity for enhancer-binding sequences. The UV-visible spectrum of purified MmoR suggested an iron coordination like other GAF domain, and that ATP is essential for the initiation of enhancer elements. Binding assays indicated that these interactions are blocked by the copper ion. These results provide novel insights into gene regulation of methanotrophs.
One for all—human kidney Caki-1 cells are highly susceptible to infection with corona- and other respiratory viruses
Authors: Daniels Alison , Fletcher Sarah , Kerr Holly E. M. , Kratzel Annika , Pinto Rute Maria , Kriplani Nisha , Craig Nicky , Hastie C. James , Davies Paul , Digard Paul , Thiel Volker , Tait-Burkard Christine 
Published: September 2023

ABSTRACT

In vitro
investigations of host-virus interactions are reliant on suitable cell and tissue culture models. Results are only as good as the model they are generated in. However, choosing cell models for
in vitro
work often depends on availability and previous use alone. Despite the vast increase in coronavirus research over the past few years, scientists are still heavily reliant on: non-human, highly heterogeneous or not fully differentiated, or naturally unsusceptible cells requiring overexpression of receptors and other accessory factors. Complex primary or stem cell models are highly representative of human tissues but are expensive and time-consuming to develop and maintain with limited suitability for high-throughput experiments.Using tissue-specific expression patterns, we identified human kidney cells as an ideal target for severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) and broader coronavirus infection. We show the use of the well-characterized human kidney cell line Caki-1 for infection with three human coronaviruses (hCoVs):
Betacoronaviruses
SARS-CoV-2 and Middle Eastern respiratory syndrome coronavirus and
Alphacoronavirus
hCoV 229E. Caki-1 cells show equal or superior susceptibility to all three coronaviruses when compared to other commonly used cell lines for the cultivation of the respective virus. Antibody staining against SARS-CoV-2 N protein shows comparable replication rates. A panel of 26 custom antibodies shows the location of SARS-CoV-2 proteins during replication using immunocytochemistry. In addition, Caki-1 cells were found to be susceptible to two other human respiratory viruses, influenza A virus and respiratory syncytial virus, making them an ideal model for cross-comparison for a broad range of respiratory viruses.

IMPORTANCE
Cell lines remain the backbone of virus research, but results are only as good as their originating model. Despite increased research into human coronaviruses following the COVID-19 pandemic, researchers continue to rely on suboptimal cell line models of: non-human origin, incomplete differentiation, or lacking active interferon responses. We identified the human kidney Caki-1 cell line as a potential target for severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2). This cell line could be shown to be infectable with a wide range of coronaviruses including common cold virus hCoV-229E, epidemic virus MERS-CoV, and SARS-CoV-2 as well as other important respiratory viruses influenza A virus and respiratory syncytial virus. We could show the localization of 26 SARS-CoV-2 proteins in Caki-1 cells during natural replication and the cells are competent of forming a cellular immune response. Together, this makes Caki-1 cells a unique tool for cross-virus comparison in one cell line.