bluuebunny/crossref_metadata_2025_split huggingface dataset:
Investigation of various plant protein ingredients for processed cheese analogues: physical properties and microstructure compared with milk proteins
Authors: Lu Di , Roy Debashree , Acevedo-Fani Alejandra , Singh Harjinder , Ye Aiqian 
Published: January 2025

Abstract
This study evaluated various structural and physical properties of several plant proteins in the context of processed cheese analogues (PCAs). A total of 9 plant protein sources were selected to formulate PCA samples. The samples were processed at 90 °C for 10 min using either a rapid visco analyzer or water bath for different tests. Rheological analysis revealed that PCA samples formulated with plant proteins all exhibited solid-like behaviour. PCAs containing legume proteins had a higher storage modulus (G’) than that of rennet casein (RC) cheese samples, while canola protein samples showed the lowest G’ values. Zein-based PCA had the highest hardness and chewiness but softened when subjected to heat during the stretchability test. In contrast, PCAs containing chickpea, mung bean, or pea proteins exhibited similar hardness to RC-based cheeses but had poorer springiness, cohesiveness, and resilience. Plant protein-based PCAs also lacked melting and stretchability properties due to the absence of a continuous protein network. When ranking all proteins in PCAs based on viscosity, rheological, and textural properties, lentil protein scored the highest, followed by hemp and quinoa proteins, performing most similarly to casein protein. The presented comparison of different plant proteins in PCAs provides valuable insights for cheese analogue development.
Increasing the stability of ascorbic acid through encapsulation in food-grade vesicles: an approach for nutritional improvement
Authors: Tissera Carolina E , Barnetche María Eugenia , Silva Oscar Fernando , Fernández Mariana A 
Published: January 2025

Abstract
l-ascorbic acid (AA), vitamin C, is a potent antioxidant, important in combating bacterial infections, facilitating detoxification reactions, and contributing to the formation of collagen in fibrous tissue, skin, and capillaries. AA is obtained by humans from food. However, its sensitivity to external stimuli, such as light, heat, and oxygen, limits the processing of foods that contain it. The objective of this work was to improve the stability of AA through encapsulation in food-grade nanovesicles. AA was encapsulated in amounts that met the recommended daily intake of vitamin C. The impact of food processing-related parameters such as temperature, ionic strength, pasteurisation, freezing, and freeze-drying on niosomal systems was investigated. It was found that the shelf life of AA could be extended to at least 40 days after freeze-drying and rehydration, maintaining adequate amounts of the vitamin. Under this treatment, the integrity and morphological characteristics of AA-loaded niosomes were preserved. Furthermore, the system retained at least 87% of the antioxidant capacity, making it an interesting alternative for possible use in food fortification.
Deacidification characteristics of Issatchenkia terricola WJL-G4 with a special focus on bioactive compounds and antioxidant activities during currant juice and wine processing
Authors: Guo Huihui , Lu Yiwei , Yang Guang , Gao Liping , Wang Jinling 
Published: January 2025

Abstract
Issatchenkia terricola WJL-G4 (WJL-G4), known for its strong deacidification ability, was selected for processing currant juice (CJ) and wine to address the issue of excessive acidity. This research aimed to examine how acid-reducing fermentation (ARF) impact the quality of juices and wines in terms of bioactive compound content and antioxidant capacities. In CJ (black, red, white), citric acid degradation reached 80.55%, 79.60%, and 77.51% after 80, 84, and 88 hr of ARF, respectively. The results indicated a decrease in bioactive compounds in juices post-ARF, whilst the impact on wines was less pronounced. However, concentrations of isovitexin, epicatechin, sinapic acid, coumaric acid, syringic acid, and rutin were increased after ARF. Significantly, the antioxidant capacities of red currant wine with WJL G4 were markedly higher compared to red currant wine without WJL-G4. This study revealed that I. terricola WJL-G4 ARF reduced organic acid content whilst preserving bioactive compounds and enhancing wine colour.
Digitalisation, bioinformatics, and delivery systems in sustainable nonthermal extraction of proteins
Authors: Režek Jambrak Anet , Nutrizio Marinela , Dukić Josipa , Djekić Ilija , Vinceković Marko , Jurić Slaven , Pataro Gianpiero , Tiwari Brijesh , Goksen Gulden , Semenčić Mojca Čakić , Slavica Anita , Sabljak Iva , Diminić Janko , Pecina Ena , Sabol Andrija , Tixier Anne-Sylvie Fabiano , Donsi Francesco 
Published: January 2025

Abstract
Sustainable development integrates social, economic, technological, scientific, and environmental challenges through the framework of 17 Sustainable Development Goals. Industry 4.0, digitalisation, plant-based processing, environmental challenges, and alternative protein sources are vastly researched and present the foundation of innovative processing in the food processing industry. The exploitation of agroindustrial discards and plant-based by-products as an alternative source of valuable nutrients, including proteins, highlights the potential of upcycling (new economic value) while addressing sustainability challenges. Within the idea of nonthermal extraction of proteins and its process digitalisation, it is crucial to innovate, connect elements of Industry 4.0 and valorise the term sustainability. Innovative approaches in extractions such as ultrasound, plasma, pulsed electric fields, high-intensity pulses, and others, have an energy-saving effect with limited use of toxic chemicals and/or solvents. Usage of software for solvent selection, incorporating digitalisation, and development of protein and peptide databases can help in the prediction, optimisation, and efficiency of nonthermal extractions of proteins from agroindustrial discards. It is also important to select optimal delivery systems like electrospinning, electrospraying, or encapsulation as useful and efficient processes. The environmental, economic, and societal impact of innovative processing needs to be monitored and valorised using specific tools. Life cycle assessment methodology assesses the environmental impact throughout the life cycle of a commercial product, service, or process. The current review shows that extracting protein from agroindustrial discards and by-products of plant and animal origins is a complex matter. The need for a comprehensive approach that considers various viewpoints on extraction is suggested.
The green method of enriching linseed oil with antioxidants as well as flavour and aroma components from herbs and spices
Authors: Hrebień-Filisińska Agnieszka M , Aydin Merve , Proch Michał 
Published: January 2025

Abstract
The influence of ultrasonic maceration on the extraction of antioxidant and flavour ingredients from cloves and sage directly into linseed oil was investigated. The ingredients were determined by high-performance liquid chromatography. The antioxidant activity of 2,2-diphenyl-1-picrylhydrazyl (DPPH), total polyphenols, flavonoids, oxidation level, and organoleptic quality was examined. The clove macerate had the best antioxidant, flavour, and aroma properties (eugenol 406.9 mg/100 g, DPPH 86%, polyphenols 447.9 mg/100 g, and flavonoids 7.39 mg/100 g). Next in the ranking was sage macerate (carnosic acid 29.58 mg/100 g, carnosol 4.57 mg/100 g, DPPH 21%, polyphenols 109.6 mg/100 g, and flavonoids 5.54 mg/100 g). Ultrasonic extraction from appropriate plant species makes it possible to obtain, in a simple and ecological way, oil macerates rich in phytochemicals, which can be a functional addition to other food or cosmetic products.
High pressure and heat-induced potato protein nanocomplexes as carriers for curcumin: influence of pH and curcumin addition pathway
Authors: Khati Paula , Marchesseau Sylvie , Cunault Charles , Picart-Palmade Laetitia , Chevalier-Lucia Dominique 
Published: January 2025

Abstract
This study aimed to assess the potential of high hydrostatic pressure (HHP) and heat treatment (HT) in facilitating the formation of nanoassemblies of potato proteins (PPs) and curcumin to protect this sensitive bioactive compound. Curcumin was introduced into patatin-rich protein dispersions (4% wt/wt) according to two pathways: before (pathway 1) or after (pathway 2) HHP treatment (400 or 600 MPa for 8 hr at pH 6 and pH 7) or HT (55 °C for 5 min at pH 6) to optimize curcumin–protein complexation efficiency (CE). Treatments increased PP surface hydrophobicity which facilitated curcumin binding and protein aggregation, as confirmed by protein particle size increase. The highest CE was obtained with pathway 1 at 600 MPa pH 7, while HT limited curcumin complexation. Whatever the pathway, the antioxidant activity of the processed dispersions treated at 600 MPa for 8 hr or 55 °C for 5 min was increased compared to untreated ones and preserved after 10 days of storage at 4 °C. These nanocomplexes could potentially be applied in functional foods.
Bioavailable fraction from the edible leaf of Albizia lebbeck (L.) Benth. inhibits neurotoxicity in human microglial HMC3 cells and promotes lifespan in Caenorhabditis elegans
Authors: Phoraksa Onuma , Vongthip Wudtipong , Juntranggoor Pichakorn , Maiuthed Arnatchai , Tuntipopipat Siriporn , Charoenkiatkul Somsri , Tencomnao Tewin , Muangnoi Chawanphat , Sukprasansap Monruedee 
Published: January 2025

Abstract
Neurodegeneration is involved in the deterioration and death of cells in the central nervous system. Albizia lebbeck (L.) Benth. has exhibited antioxidant and health benefits. This study focused on the protective effect and underlying mechanism of the bioavailable fraction of A. lebbeck leaf (BAL) against toxicity of glutamate-induced endoplasmic reticulum (ER) stress and cell death in human microglial HMC3 cells, as well as evaluated the longevity and antioxidant effects of BAL in Caenorhabditis elegans. The BAL was obtained from the in vitro digestion of A. lebbeck leaf coupled with Caco-2 cells. Results showed that treating HMC3 cells with BAL attenuated glutamate-induced ER stress and apoptosis by decreasing the protein expressions of calpain1, caspase-12, Bax, cytochrome c, and cleaved caspase-9 while increasing the antiapoptotic Bcl-2. Additionally, LC–MS/MS results showed that BAL contained flavonoids and carotenoids such as quercetin-3β-D-glucoside, robinetin, vitexin, kaempferol, kuromanin, daidzein, tanshinon I, nootkatone, rutin, and luteolin. We further investigated molecular docking to illustrate these bioactive compounds on apoptosis-related mechanisms. The results demonstrated that luteolin, kaempferol, and nootkatone inhibited Bax, cytochrome c, and caspase-9 functions. We found that BAL also extended the lifespan of C. elegans and distinctly increased survival in response to juglone-induced oxidative stress. Interestingly, treating C. elegans with BAL could increase superoxide dismutase 3 expression, relating to the anti-stress response. These findings suggest that BAL possesses beneficial function in neuroprotection and longevity, supporting its potential for preventing age-related neurodegeneration.
The pharmacodynamics of fosfomycin in combination with meropenem against Klebsiella pneumoniae studied in an in vitro model of infection
Authors: MacGowan Alasdair P , Griffin Pippa , Attwood Marie L G , Daum Aimee M , Avison Matthew B , Noel Alan R 
Published: January 2025

Abstract

Background
Intravenous fosfomycin is used in combination with other antimicrobials for the management of severe and/or multidrug resistant Gram-negative infection. We used an in vitro pharmacokinetic model to study the combination of fosfomycin plus meropenem.

Methods
Six Klebsiella pneumoniae fosfomycin MICs 8–1024 mg/L, meropenem MICs 0.06–&gt;1024 mg/L were employed. A dilutional pharmacokinetic model was used to generate fosfomycin exposure ranges up to a fAUC/MIC 500. Exposure-ranging experiments were repeated in the presence of meropenem at exposures associated with 2 g 8-hourly human dosing for strains with meropenem MICs ≥32 mg/L and at half the bacteriostatic fT &gt; MIC for strains with MICs &lt;32 mg/L. The log change in bacterial burden from the initial inoculum after 24 h drug exposure was taken as the primary endpoint and fAUC/MIC ratios for antibacterial effects were calculated. The risk of emergence of resistance was assessed by measurement of the population profiles.

Results
Fosfomycin fAUC/MIC for bacteriostatic effect at 24 h were &gt;500 for 5/6 K. pneumoniae strains. Meropenem fT &gt; MIC for static effect were 16.6%–77.9% for the strains with meropenem MIC ≤ 64 mg/L. Strains with MICs of &gt;1024 mg/L were not tested. Fosfomycin fAUC/MICs in the presence of meropenem were all reduced and for 5/6 strains the fAUC/MIC for static effect was &lt;10 and &lt;30 for a 2 log drop. Addition of meropenem suppressed changes in fosfomycin population profiles. There were no changes in meropenem population profiles exposed to the combination.

Conclusion
Addition of meropenem to fosfomycin had a dramatic impact on the fosfomycin fAUC/MIC exposures required for bacteriostatic and bactericidal effects and suppressed emergence of fosfomycin resistance.
Antibiotic use at the Centre Hospitalier Universitaire de Zone d'Abomey Calavi/Sô-Ava (CHUZ/AS) in Benin: a point prevalence survey
Authors: Gnimavo Morelle Sèssiwèdé , Boya Bawa , Mudenda Steward , Allabi Aurel Constant 
Published: January 2025

Abstract

Background
Antimicrobial stewardship promotes the appropriate use of antibiotics to prevent the emergence and spread of antimicrobial resistance. This study evaluated the use of antibiotics using a point prevalence survey at the Centre Hospitalier Universitaire de Zone d'Abomey Calavi/Sô-Ava (CHUZ/AS) in Benin.

Methods
This cross-sectional study utilized the WHO point prevalence survey methodology for monitoring antibiotic use among inpatients in hospitals. The survey was conducted from 11 January 2022 to 19 January 2022 among hospitalized patients before 8:00 a.m. on the day of the survey.

Results
Of the 111 inpatient medical files reviewed, the prevalence of antibiotic use was 82.9%. The number of antibiotics received per patient ranged from 1 to 5, with a mean of 2.45 ± 1.11 and a median of 2. The most commonly prescribed class of antibiotics was beta-lactams (46.7%), aminoglycosides (20.6%) and nitroimidazoles (19.7%). According to the WHO AWaRe classification, 30.4% of inpatients received the Access group of antibiotics and 44% received a combination of Access and Watch group antibiotics; treatment was empiric in 94.5% of encounters. Only 22.7% of patients were treated based on microbiological examination/culture and sensitivity testing.

Conclusions
This study found a high prevalence of antibiotic use among inpatients at the CHUZ/AS Tertiary Care Hospital in Benin. The most prescribed antibiotics were ampicillin, metronidazole and ceftriaxone. Consequently, the study found a low use of culture and sensitivity testing to guide treatment, particularly in the paediatric and surgical population, and the preference for broad-spectrum antibiotics suggests that antibiotic use at the CHUZ/AS Tertiary Care is not optimal. Therefore, antimicrobial stewardship programmes, policies and guidelines must be instigated and strengthened to address these gaps and promote rational use of antibiotics.
Interactive effects between yields of Bt and non-Bt corn and planting dates in the southeastern United States
Authors: Reay-Jones Francis P F , Buntin G David , Reisig Dominic D 
Published: January 2025

Abstract
Transgenic corn (Zea mays L.) expressing insecticidal toxins from Bacillus thuringiensis (Bt) helps to control or suppress injury from a range of target insect pests. This study summarizes the yield benefits of Bt corn from field trials in Georgia, North Carolina, and South Carolina evaluating Bt and non-Bt corn hybrids from 2009 to 2023. For Cry1A.105 + Cry2Ab2 and Cry1Ab + Cry1F Bt corn, the percentage increase in yield between Bt and non-Bt corn hybrids did not vary significantly among years or states. Across years, the percentage increase in yield for both Bt traits relative to non-Bt corn hybrids increased with planting date, indicating that the yield benefit of planting Bt corn was greater later in the growing season. On average, Bt hybrids in both families had a significantly higher yield compared to their non-Bt pairs, with yields declining in later planting dates. Yields declined with increasing kernel injury from Helicoverpa zea (Boddie) and leaf injury from Spodoptera frugiperda (J. E. Smith) in both Bt and non-Bt hybrids. While insect pressure increased in later planted corn trials, identifying causal relationships between increases in insect damage and associated yield losses in corn is challenging given the numerous factors that influence corn yield during the season. In addition to illustrating the highly variable yield benefits of planting Bt corn, our study suggests that yield increases in Bt corn may occur more frequently than previously documented in the southeastern United States.
Attributes of Pediatric Music Therapists: A Qualitative Analysis of Parent Descriptions
Authors: Harman Elizabeth , Stegenga Kristin , Robb Sheri L 
Published: January 2025

Abstract
Therapist attributes are known to contribute to positive therapeutic outcomes and are important to effective training and clinical practice. Although well researched in psychology and play therapy, few studies have directly explored music therapist attributes. To explore and understand these descriptions, we conducted a secondary analysis of parent interview data from a multisite trial investigating a music therapist-delivered intervention for young children with cancer and their parents. We used qualitative descriptive analysis to identify music therapists’ attributes as described by parents who participated in the intervention. Our inductive analysis of 28 interviews revealed 135 descriptors of music therapists. We discovered that these descriptions grouped naturally using essential therapist attributes of supportive, attuned, and nonjudgemental, as identified in the extant literature. This analysis highlights music therapists’ attributes perceived as central to their work with parents and young children in cancer settings.
Antimicrobial Activity of Imipenem/Relebactam and Comparator Agents Against Gram-Negative Isolates Collected From Pediatric Patients: SMART 2018–2022 Global Surveillance
Authors: DeRyke C Andrew , Wise Mark G , Bauer Karri A , Siddiqui Fakhar , Young Katherine , Motyl Mary R , Sahm Daniel F 
Published: January 2025

Abstract

Objectives
To evaluate the in vitro susceptibility of recent Gram-negative pathogens collected from pediatric patients to imipenem/relebactam (IMI/REL) and comparator agents.

Methods
From 2018 to 2022 254 hospitals in 62 countries collected Enterobacterales or Pseudomonas aeruginosa isolates from patients &lt;18 years old as part of the SMART global surveillance program. Minimum inhibitory concentrations (MIC)s were determined using CLSI broth microdilution and interpreted with 2024 CLSI breakpoints. Most isolates non-susceptible to IMI/REL were queried for their acquired β-lactamase content.

Results
Overall, 96.8% of all non-Morganellaceae Enterobacterales (NME) isolates from pediatric patients (n = 12 060) were IMI/REL-susceptible. Most NME were also susceptible to imipenem alone (93.9%), meropenem (96.0%), and ertapenem (94.4%); isolates were less susceptible to piperacillin/tazobactam (82.8%), cefepime (76.3%), and ceftazidime (74.4%). Non-Morganellaceae Enterobacterales collected in Asia were the least susceptible to IMI/REL (91.6%), while those from Australia/New Zealand were the most (99.3%). Imipenem/relebactam was equally potent against NME isolates regardless of infection source, hospital ward, age, and length of hospitalization. In total, 90.8% of all Pseudomonas aeruginosa isolates (n = 3046) were IMI/REL-susceptible; ceftolozane/tazobactam also inhibited &gt;90% of the P. aeruginosa. Regionally, P. aeruginosa isolates from Eastern Europe were least susceptible to IMI/REL. Molecular characterization revealed that, globally, most resistance to IMI/REL among the NME could be attributed to the presence of NDM-type metallo-β-lactamases, while no acquired β-lactamases were detected in approximately half the IMI/REL non-susceptible P. aeruginosa examined.

Conclusion
Based on in vitro data, IMI/REL represents a good therapeutic option for most hospitalized pediatric patients infected with common Gram-negative pathogens.
A domain calculus based on Heyting-valued logic
Authors: Lastovicka Jan 
Published: January 2025

Abstract
We present a domain calculus for databases that store information in the form of $L$-relations, where $L$ is a complete Heyting algebra. First-order formulas serve as a query language for such databases. However, the set of formulas needs to be restricted to those that are safe to use. The main motivation is to study databases in which incomplete information is modeled by $L$-relations.
Moving-mesh non-ideal magnetohydrodynamical simulations of the collapse of cloud cores to protostars
Authors: Mayer Alexander C , Zier Oliver , Naab Thorsten , Pakmor Rüdiger , Caselli Paola , Ivlev Alexei V , Springel Volker , Walch Stefanie 
Published: January 2025

ABSTRACT
Magnetic fields have been shown both observationally and through theoretical work to be an important factor in the formation of protostars and their accretion discs. Accurate modelling of the evolution of the magnetic field in low-ionization molecular cloud cores requires the inclusion of non-ideal magnetohydrodynamics (MHD) processes, specifically Ohmic and ambipolar diffusion and the Hall effect. These have a profound influence on the efficiency of magnetic removal of angular momentum from protostellar discs and simulations that include them can avoid the ‘magnetic-braking catastrophe’ in which discs are not able to form. However, the impact of the Hall effect, in particular, is complex and remains poorly studied. In this work, we perform a large suite of simulations of the collapse of cloud cores to protostars with several non-ideal MHD chemistry models and initial core geometries using the moving-mesh code arepo. We find that the efficiency of angular momentum removal is significantly reduced with respect to ideal MHD, in line with previous results. The Hall effect has a varied influence on the evolution of the disc which depends on the initial orientation of the magnetic field. This extends to the outflows seen in a subset of the models, where this effect can act to enhance or suppress them and open up new outflow channels. We conclude, in agreement with a subset of the previous literature, that the Hall effect is the dominant non-ideal MHD process in some collapse scenarios and thus should be included in simulations of protostellar disc formation.