bluuebunny/crossref_metadata_2025_split huggingface dataset:
Amanita thiersii and Amanita foetens are closely related but genetically and geographically distinct species, leaving the origins of A. thiersii and its range expansion enigmatic
Authors: Dunkirk Nora , Wang Yen-Wen , Drott Milton T. , Elmore Holly , Robledo Gerardo , Tulloss Rodham E. , Pringle Anne 
Published: July 2023

Background: The decomposer Amanita thiersii was originally described from a Texas lawn. Over time the species appears to have spread its range, but whether A. thiersii is an introduced and invading fungus or a native expanding its range remains an open question. A striking morphological similarity between A. thiersii and the Argentinian A. foetens led us to question whether the two species are the same. We hypothesized A. thiersii was simply an A. foetens introduced from Argentina. Methods: We first compared the original species descriptions of both taxa. Next, we used databases associated with iNaturalist and Mushroom Observer to plot the global ranges of A. thiersii and A. foetens, revealing new reports of A. thiersii in Mexico and an expanded range in the United States of America. Next, we sequenced three genomes: an A. thiersii specimen from the U.S.A., an isotype of A. foetens, and an Argentinian specimen tentatively identified as A. thiersii. We reconstructed phylogenies using our own and publicly available data of other Amanita species. Because the genetic diversity of A. thiersii in the U.S.A appears to be very low, we also searched for mating type loci. Results: Macroscopic descriptions suggest the two taxa are distinguishable by mushroom stature and the decoration of the stipe. The geographic ranges of the two taxa seem distinct and not overlapping, although the inconsistent names used by database users causes confusion. Phylogenies suggest the genomes of mushrooms collected in U.S.A. are different from Argentinian genomes. We discovered an individual which appears to have a mating type locus present in one nucleus of the dikaryon and absent from the second nucleus. Conclusions: While A. thiersii and A. foetens appear strikingly similar, each is morphologically, geographically and genetically distinct, leaving the question of whether A. thiersii is native or introduced to the U.S.A. unanswered.
Unilateral adrenalectomy for primary bilateral macronodular adrenal hyperplasia
Authors: Chevais A. , Elfimova A. R. , Derkatch D. A. , Romanova A. N. , Ebzeeva A.  K. 
Published: July 2023

BACKGROUND: To date, surgical treatment is an effective treatment of hypercortisolism in primary bilateral macronodular hyperplasia (PBMAH). Due to the bilateral lesion, the most common treatment in Russia is the bilateral adrenalectomy, which requires the appointment of hormone replacement therapy. The results of various studies on the effectiveness of unilateral adrenalectomy (UA) in PBMAH are rather discordant.AIM: The objective of the study was to assess the outcomes of UA in patients with PBMAH, accompanied by mild autonomous cortisol excess (MACE) or overt Cushing’s syndrome (CS).MATERIALS AND METHODS: 44 patients with PBMAH and a manifest form of CS (n=21) MACE with comorbid conditions (n=23) underwent UA in the surgical department of the Endocrinology Research Centre. The dynamics of laboratory parameters (cortisol after overnight dexamethasone suppression test (cortisol ONDST) urinary free cortisol (СКМ), ACTH), as well as the course of comorbid diseases (diabetes mellitus, arterial hypertension (AH), metabolic disorders) were assessed before and after 6 months of surgical intervention. The cut-off level of blood cortisol (8:00–9:00) during the first day after OA was determined as a predictor of the development of adrenal insufficiency (AI) in the early postoperative period.RESULTS: 6 months after OA, laboratory parameters improved in 40/44 (91%, 95% CI: 78%-97%) cases: cortisol ONDST decreased from 466 [173; 652] to 86 [61; 149] nmol/l, p=<0.01, СКМ dropped from 840 [468; 1892] to 267 [204; 432] nmol/day, p=<0.01), while ACTH increased gradually from 1 [1; 2.1] to 8.3 [2.6; 15.0] pg/ml, p=<0.01), which indicates the effectiveness of UA. Statistically significant changes in BMI were also obtained (before UA — 30.8 [27.5; 34.4], after — 28.5 [23.9; 32.2] kg/m2 (p <0.01)). When analyzing the long-term outcomes of UA on carbohydrate metabolism, a decrease in the level of HbA1c from 7.0 [6.3; 7.7] to 6.0 [5.6; 6.7] %, (p<0.01) was observed. Further, there was an improvement in the course of AH. After UA, antihypertensive therapy was completely discontinued in 27.7% of cases, and a reduction in the number of antihypertensive drugs was carried out in 44.4%. The level of cortisol in the early postoperative period less than 325 nmol/l was the strongest factor predicting the potential adrenal failure (AUC=0.96).CONCLUSION: UA can be recommended as the first line treatment of PBMAH with CS due to the high probability of longterm remission of hypercortisolism and improvement in the course of comorbid diseases.
Key principles of drug therapy in patients with chronic myeloid leukemia
Authors: Zhuravlev A. V. , Knysh O. I. 
Published: July 2023

Objective: to summarize scientific information about the basic principles of modern drug therapy for patients with chronic myeloid leukemia (CML) considering their individual characteristics.Material and methods. The basis of the study included modern scientific articles and clinical guidelines on CML diagnosis and treatment (2021), State Register of Medicines (SRM) of the Russian Federation, instructions for the use of medicines. The following methods were used: structural analysis, analytical method, content analysis, retrospective analysis, systematic approach, situational-logical and graphical methods of analysis.Results. The analysis made it possible to summarize scientific information about the basic principles of drug therapy for patients suffering from CML. It was revealed that the problem of CML therapy today is relevant, since every year there is an increase in the incidence of this nosology. Currently, the most significant is the prescription of tyrosine kinase inhibitors (TKIs), since they have pronounced effects and are well tolerated by patients. Therapy for CML in TKIs prescription consists of several lines. Imatinib is the first line therapy because it has better safety profile. There are combinations with imatinib; for example, it is used together with interferon alfa, which allows, in some cases, to increase the response to treatment. The following drugs are used in the second line: nilotinib, dasatinib, bosutinib, ponatinib. If TKI therapy is ineffective, it is possible to prescribe standard chemotherapy, interferon therapy, or bone marrow transplantation in the absence of contraindications. Studies are underway on the possibility of using and including in clinical guidelines such drugs as arsenic trioxide, decitabine, omacetaxime, inhibitors of farnesyl transferases, granulocyte-macrophage factors, antitumor vaccines. The analysis of SRM identified 27 trade names for TKIs, the share of domestic drugs was 60%. There were no Russian analogues for bosutinib and ponatinib in SRM, which are recommended for use in case of ineffective TKI therapy of previous lines.Conclusion. The study of drug provision for CML patients is an urgent task for pharmaceutical practice and for the healthcare system as a whole. Currently, the acute issues are the individual approach to the treatment of each CML patient considering concomitant diseases, and the search for new, more effective drugs that can increase the life expectancy and quality of life of patients suffering from this disease.
Methodological approach to the formation of the list of high-tech medical care types
Authors: Zheleznyakova I. A. , Volkova O. A. , Fedyaev D. V. , Zuev A. V. , Plakhotnik O. S. , Trifonova G. V. , Zueva Yu. S. , Alexandrov I. A. , Omelyanovskiy V. V. 
Published: July 2023

Background. The implementation of individual methods of high-tech medical care (HTMC) with methods of specialized medical care within the framework of a phased transition to payment methods by diagnostic-related groups (DRGs), on the one hand, significantly expanded the availability of HTMC methods for the population. Still, it created a situation with duplication of individual treatment methods in the list of HTMC types and DRGs. Amendments to the Order of the Ministry of Health of the Russian Federation (MH RF) of August 1, 2017 No. 484n, regulating the revision of HTMC types list in terms of excluding treatment methods and (or) HTMC types in case of their duplication in the context of HTMC groups and/or DRGs, marked the beginning of large-scale work in this area and required methodological ensuring the processes of forming HTMC types list, including in terms of its revision.Objective: development of a methodological approach to the formation of HTMC types list (using the example of the list for 2023). Material and methods. An analysis of legal documents regulating the HTMC availability in the Russian Federation was carried out, including the list of medical services approved by the Order of the MH RF of October 13, 2017 No. 804n, clinical recommendations for certain nosological entities, the International Classification of Diseases (10th revision), methodological recommendations on ways to pay for medical care at the expense of the compulsory health insurance (CHI) and appendices to them (decoders of DRGs for payment of medical care provided in inpatient and daytime hospital conditions), posted on the official website of the Federal CHI Fund. The frequency of the use of certain HTMC methods and DRGs was analyzed on the basis of impersonalized information from the database of registers of bills for specialized medical care, including HTMC for 2021–2022.Results. A methodological approach to the revision of HTMC types list was developed. It included its primary analysis, expert discussion of the obtained results, consideration by the Interdepartmental Council of the MH RF of proposals agreed with experts on each HTMC method submitted for discussion, followed by a decision on the appropriateness of the proposed changes, and recalculation of the standard of financial costs for HTMC and/or basic tariff for DRGs.Conclusion. The proposed methodological approach makes it possible to unify the process of revising the HTMC types list, including the exclusion of duplicate treatment methods and/or HTMC types in the sections of HTMC list types and/or in DRGs, as well as treatment methods missing in clinical recommendations, etc. in order to bring the HTMC types list in accordance with legal documents regulating the provision of medical care in the Russian Federation.
Infection with Pythium flevoense in a harbour porpoise (Phocoena phocoena) as a novel cause of dermatitis in marine mammals
Authors: Kroeze Edwin JB Veldhuis , Elk Cornelis E. van , de Bildt Marco W.G. van , Run Peter R.W.A. van , Foster Geoffrey , Abou-Chakra Nissrine , Hare Rasmus Krøger , Kuiken Thijs 
Published: July 2023

Abstract
The oomycete Pythium flevoense was diagnosed as the etiology of dermatitis in a young adult female harbour porpoise (Phocoena phocoena) that had been trapped in a pound net in a temperate saltwater environment. Disease from Pythium sp. infection—pythiosis—is infrequently diagnosed in humans, horses, dogs, cattle, and few other mammalian species. Pythiosis is typically associated with exposure to tropical or subtropical freshwater conditions, and typically caused by Pythium insidiosum. However, until now, pythiosis has been reported in neither marine mammals nor temperate saltwater conditions, and P. flevoense is not known as an etiology of pythiosis in mammals. This porpoise developed generalised dermatitis despite treatment and euthanasia was necessary. Histopathological evaluation revealed a chronic active erosive dermatitis, with intralesional hyphae morphologically consistent with a Pythium sp. PCR analysis and sequencing of affected skin matched Pythium flevoense with a 100% similarity to the reference strain. Additional diagnostics excluded other pathogens as etiology. Based on this case report, P. flevoense needs to be considered as a mammalian pathogen. Furthermore, harbour porpoises and possibly other marine mammals may be at risk of infection with P. flevoense, and pythiosis should be included in the differential diagnosis of dermatitis in marine mammals.
IEC-YOLOX:A Lightweight Object Detection Algorithm for UAV Target Detection
Authors: Song Qingzeng , Xu Shuo , Xue Yongjiang , Yu Jing 
Published: July 2023

Abstract

Recently, unmanned aerial vehicles (UAVs) have been widely used in industries such as transportation and agriculture for capturing scenes. Aerial images taken by UAVs are one of the most widely used scenario for object detection applications. However, since UAV images are captured from high altitude, the target scale of UAV images is inconsistent compared with ordinary images as the background is more complex and the target size is smaller. To address this problem, we proposed a new network model IEC-YOLOX, which is based on YOLOX. The model adds a detection layer to YOLOX-tiny to enable the detection of small targets and utilizes iAFF to fuse features of different scales. The backbone network CSPDarknet is replaced with Extended-ELAN, which reduces the number of parameters by one-fifth. Additionally, a lightweight attention mechanism called CBAM is added to the new backbone network.This paper uses VisDrone 2019 as the experimental data. The result of our YOLOX-tiny network structure's mAP@50 is 35.3$\%$, and the number of parameters is 4.29M, which is about 4.3$\%$ higher than the baseline model (YOLOX-tiny) in terms of mAP@50, and the number of parameters has been reduced by 0.74M.
Application of machine learning approaches in the computation of energy dissipation over rectangular stepped spillway
Authors: Pujari Saurabh , Kaushik Vijay , Awasthi Noopur , Gupta Shailesh Kumar , Kumar S. Anbu 
Published: July 2023

Abstract

The stepped spillway of a dam is a crucial element that serves multiple purposes in the field of river engineering. Research related to flood control necessitates an investigation into the dissipation of energy over stepped spillways. Previous research has been conducted on stepped spillways in the absence of baffles, utilizing diverse methodologies. This study employs machine learning techniques, specifically support vector machine (SVM) and regression tree (RT), to assess the energy dissipation of rectangular stepped spillways incorporating baffles arranged in different configurations and operating at varying channel slopes. Empirical evidence suggests that energy dissipation is more pronounced in channels with flat slopes and increases proportionally with the quantity of baffles present. Statistical measures are employed to validate the constructed models in the experimental investigation, with the aim of evaluating the efficacy and performance of the proposed model. The findings indicate that the SVM model proposed in this study accurately forecasted the energy dissipation, in contrast to both RT and the conventional method. This study confirms the applicability of machine learning techniques in the relevant field. Notably, it provides a unique contribution by predicting energy dissipation in stepped spillways with baffle configurations.
Multi-agent Iot-based System for Intelligent Vehicle Traffic Management
Using Wireless Sensor Networks
Authors: Kumar Golconda Ravi , Latha S. Bhargavi , Shaker Reddy Pundru Chandra , Sucharitha Yadala 
Published: July 2023

Aims:
Integrated computing technologies such as the Internet of Things (IoT), Multi-
Agent Systems (MAS), and automatic networking to deliver Internet of Vehicles (IoV) applications.

Methodology:
The main objective of this paper is to combine MAS with IoT or IoV a new paradigm
within its Cypher Physical System (CPS) for intelligent car applications. We proposed
the MAS algorithm and applied it to control traffic lights at multiple intersections. When using
MAS together with scattered computing architectures, IoV can achieve higher efficiency. The
proposed combination is based on the independent knowledge, adaptability, assertiveness, and
responsiveness that can be used in wireless sensor paradigms to bring new remedies. Smart
products will explore further advancements and diverse mobility capabilities.

Results:
IoT provides an appropriate atmosphere for connecting with MAS concepts and programs
in addition to providing reliable, adaptable, efficient, and intelligent solutions in the automotive
network. In addition, the combination of MAS with IoT and cognitive conditions
could result in scalable, automated, and smart wireless sensor solutions.

Conclusion:
We conduct experiments on three different datasets, and the results demonstrate
that MAS outperforms several state-of-the-art algorithms in alleviating traffic congestion with
shorter training time.
Sport-specific balance tests account for youth alpine skiers’ ranking
Authors: Rizzato Alex , Verdel Nina , Paoli Antonio , Supej Matej , Marcolin Giuseppe 
Published: July 2023

Objective: Alpine skiing requires complex motor skills and fine adjustments to maintain balance in dynamic and challenging conditions. This study aimed to understand whether the balance ability in unspecific (UST) and sport-specific (SST) tasks could depend on the skiers’ ranking level. The balance performance of the dominant and non-dominant limbs in the SST was also investigated.Methods: Twenty-five skiers (14.96 ± 1.61 yrs; 1.69 ± 0.69 m; 59.9 ± 9.52 kg) were divided into high-ranking (position &lt; 50) and low-ranking (position &gt; 50) groups. Subjects performed three balance conditions: static (ST), dynamic UST, and dynamic SST. Subjects stood on an unstable board over a force platform during UST. During SST, subjects wore ski boots, grasped ski poles, and each foot was clipped to an unstable board over two force plates. From the center-of-pressure (CoP) trajectory the area of the 95th percentile ellipse and the CoP mean velocity were calculated. Angular displacements were recorded by a 12-camera system, to calculate the full balance (FB), fine (FiB), and gross (GB) balance in UST and SST.Results: Balance control was higher (p &lt; 0.01) in high-ranking than low-ranking skiers only in the SST. Kinematic parameters (i.e., FB, FiB, and GB) showed a higher (p &lt; 0.001) balance performance in SST than UST independently from the group. Dominant and non-dominant limbs motion was similar (Pearson correlation, r = 0.97) in SST independently from the skiers’ ranking.Conclusion: High-ranking skiers showed better balance control and performance than low-ranking skiers only when the task was sport-specific. Therefore, we suggest testing balance under sport-specific conditions to discriminate the youth skiers’ abilities.
Characteristics of macroscopic sleep structure in patients with mild cognitive impairment: a systematic review
Authors: Liang Yahui , Liu Weihua , Wang Meizi 
Published: July 2023

ObjectivesConducting a systematic analysis of objective measurement tools to assess the characteristics of macroscopic sleep architecture in patients with mild cognitive impairment (MCI), amnestic MCI (aMCI), and non-amnestic MCI (naMCI) in order to provide sleep disorder guidance for MCI patients.MethodsPubMed, EMbase, Web of Science, Cochrane Library, CNKI, SinoMed, Wanfang Data, and VIP Data were examined to find literature relating to sleep in patients with MCI, aMCI, and naMCI, with a search time frame of build to April 2023. Following independent literature screening, data extraction, and quality evaluation by two researchers, statistical analysis was performed using RevMan 5.4 software.ResultsTwenty-five papers with 1,165 study subjects were included. Patients with MCI and aMCI were found to have altered total sleep time (TST), reduced sleep efficiency (SE), more wake-time after sleep onset (WASO), longer sleep latency (SL), a higher proportion of N1 stage and a lower proportion of N2 and N3 stage. naMCI was only found to have statistically significant differences in WASO.ConclusionsThe results of this study provide evidence for macroscopic sleep architecture abnormalities among MCI patients with sleep disorders. Maintaining a normal sleep time, improving SE, and reducing sleep fragmentation may have an association with a slowed development of cognitive impairment. Further exploration is required of the effects each component of macroscopic sleep structure after the intervention has on altered sleep disturbance and cognition in MCI, aMCI, and naMCI.Systematic review registrationhttps://www.crd.york.ac.uk/prospero/display_record.php?ID=CRD42023401937, identifier: CRD42023401937.
Melt–Vapor Phase Transition in the Aluminum–Selenium System in Vacuum
Authors: Nitsenko Alina , Volodin Valeriy , Linnik Xeniya , Burabayeva Nurila , Trebukhov Sergey 
Published: July 2023

The boundaries of liquid and vapor coexistence fields at pressures of 101.3 and 0.133 kPa were calculated based on the partial vapor pressure values of the components in the Al-Al2Se3 and Al2Se3-Se partial systems. The vapor pressures of the more volatile aluminum selenide and selenium in the above systems were determined by the isothermal version of the boiling-point method. The partial pressures of the fewer volatile components were determined by numerical integration of the Gibbs–Duhem equation. The partial and integral values of the thermodynamic functions of the formation and evaporation of solutions were calculated based on the values of the partial vapor pressure of the system components. Based on the analysis of the complete phase diagram, it was found that the purification of aluminum by vacuum distillation in a single operation can remove aluminum selenide and selenium at an appropriate rate. The distillation of selenium from melts in vacuum in the whole concentration range of the Al2Se3-Se system will proceed from the mixture of the solution with Al2Se3 cryst., with accumulation of the latter in the distillation residue.
A Lateral Flow Assay for the Detection of Leptospira lipL32 Gene Using CRISPR Technology
Authors: Natarajan Satheesh , Joseph Jayaraj , Vinayagamurthy Balamurugan , Estrela Pedro 
Published: July 2023

The clinical manifestation of leptospirosis is often misdiagnosed as other febrile illnesses such as dengue. Therefore, there is an urgent need for a precise diagnostic tool at the field level to detect the pathogenic Leptospira lipL32 gene at the molecular level for prompt therapeutic decisions. Quantitative polymerase chain reaction (qPCR) is widely used as the primary diagnostic tool, but its applicability is limited by high equipment cost and the lack of availability in every hospital, especially in rural areas where leptospirosis mainly occurs. Here, we report the development of a CRISPR dFnCas9-based quantitative lateral flow immunoassay to detect the lipL32 gene. The developed assay showed superior performance regarding the lowest detectable limit of 1 fg/mL. The test is highly sensitive and selective, showing that leptospirosis diagnosis can be achieved with a low-cost lateral flow device.