bluuebunny/crossref_metadata_2025_split huggingface dataset:
Tryptophan metabolites alleviates Intestinal Candida albicans Infection by reduction of IL-22 releasing from colonic lamina propria group 3 innate lymphoid cells and gut microbiome modification
Authors: Peng Ziyao , Zhang Jiali , Zhang Meng , Yin Liping , Zhou Ziyang , Lv Cuiting , Wang Zetian , Tang Jianguo 
Published: April 2023

Abstract
Background: Invasive candidiasis may be caused by Candida albicans (C. albicans) colonisation of the intestinal tract. Therefore, protection against invasive C. albicansinfections requires a functional intestinal epithelial barrier. Mice infected with Candida albicans in the gut and healthy mice were sampled for faecal analysis. After C. albicans infection, we found significant changes in tryptophan metabolites-kynurenic acid (KynA) and indole acrylate (IA)compared with healthy mice.
Results: We identify the mechanism by which KynA and IA ameliorate dextran sulphate sodium (DSS)-induced intestinal C. albicansinfection, based on a systematic analysis of the gut microbiota, metabolomics and transcriptome sequencing in mice. KynA and IA suppress inflammation, upregulate the expression of intestinal tight junctions and reduce IL-22 secretion of colonic lamina propria ILC3 in response of invasive Candida infection. Furthermore, we demonstrated that the gut barrier protection and ILC3 regulation mediated by KynA and IA were dependent on gut microbiota modification by transplanting faecal microbiota into ABX-treated C57BL/6J mice.
Conclusion: Our study provides microbiome-to-metabolite alterations in C. albicans gut infection and identifies gut microbiome regulation and IL-22 derived from colonic lamina propria ILC3 as novel targets for C. albicans gut infection therapies.
Data Protection in Internet of Medical Things Using Blockchain and Secret Sharing Method
Authors: Shree Shreyshi , Zhou Chen , Barati Masoud 
Published: April 2023

Abstract
Internet of Medical Things (IoMT) combines Internet of Things (IoT)with medical devices to facilitate healthcare, providing affordable solutions, and faster treatments to patients. An increase in IoMT use hasled to several security/privacy challenges, since many IoMT devices havenot been designed with security and privacy features in mind, whichmakes them vulnerable to attacks. Furthermore, as security risks andthreats affect all the layers developed for IoMT-based architectures, anIoMT network must follow stricter privacy and security specificationscompared to other IoT devices. In order to address this, we present anew IoT-based architecture to improve the data privacy, security, andintegrity leveraging distributed InterPlanetary File System (IPFS) storage and blockchain. The data captured from medical devices is split intomultiple encrypted pieces using Secret Sharing Algorithm (SSA) andthese pieces are then stored in distributed IPFS storage hosted on edgeand cloud servers, and their copies are verifiable by a blockchain network. The applied SSA method ensures that even if a piece of data iscompromised, the original data is neither leaked nor lost. Our proposedarchitecture is implemented and tested on an IoMT-based monitoring system to investigate its feasibility, scalability, and performance.
Bibliometric and visual analysis of the global treatment of affective disorders in the 21st Century: Developments, hotspots, and trends
Authors: Wei Zhizi , Zhong Chaofang 
Published: April 2023

Abstract
The treatment of affective disorders is becoming the mainstream of research, and its study has attracted extensive attention from scholars all over the world. However, there is no comprehensive review of this research. From the perspective of bibliometrics, based on 2692 articles in the Web of Science Core Collection (WOSCC) database, this paper systematically and comprehensively describes the progress and hot spots in the treatment of affective disorders. Since the 21st century, research on treating affective disorders has shown a growing trend. The results show that from 2000 to 2021, the United States was the most active country in publishing relevant articles, Harvard University was the most productive institution, and Ketter T was the most representative author in this field. However, there was little cooperation between different countries/institutions. The research in the field of affective disorder treatment can be summarized into three main research areas: the leading causes of affective disorder, the harm of affective disorder, and the treatment methods. Finally, this paper proposes some challenges and prospects for the rapid and balanced development of the field of affective disorder therapy. As far as we know, this study is the first one specialized in visualizing the treatment of affective disorders, which is conducive to identifying current research hotspots and predicting potential future research directions.
G protein signaling 1 regulator facilitate ovarian cancer development by activating NF-κB signal pathway
Authors: Shengnan Liu , Jiayan Xi , Kaisi Zhu , Li Li , Wei Huang , Shengyuan Cai , Mingjuan Xu 
Published: April 2023

Abstract
Background The RGS1 protein and its regulators are key players in the development of different malignancies. The RGS1 gene was considered as a promising biomarker for ovarian cancer prognosis, and it was hypothesized that it would encourage proliferation via the NF-κB signaling pathway.Methods The data was gathered using the Cancer Genome Atlas (TCGA) database, the Human Protein Atlas (HPA) dataset, and the gene expression omnibus (GEO) database. The role of RGS1 in the development of OC was examined utilizing the CCK-8, EDU, Transwell, real-time PCR, and Western blotting assays. In nude mice, the impact of RGS1 on OC formation was confirmed.Results RGS1 was revealed to be a major determinant of OC prognosis. Functionally, although knockdown of RGS1 had the opposite effects, ectopic expression of RGS1 increased growth capacity in vitro and triggered tumor development in vivo. Additionally, RGS1 suppression caused OC cells to stop in the G1/S phase, which is in line with changes in cell-cycle components. In terms of mechanism, RGS1 knockdown inhibited the NF-κB signaling pathway.Conclusions RGS1 can be a target for cancer treatment since it performed an oncogenic function in the development of OC, in part via controlling the NF-κB signaling pathway.
Efficacy and safety of Thoracoscopic-guided Paravertebral Block on the Early Postoperative Analgesia following Video-assisted Thoracoscopic Lobectomy: A Randomized Blinded Controlled Study
Authors: Li Zhixiong , Lin Qingshui , Lin Liangqing , Wu Qinghua , Ke Pinhui , Chen Huan , Lin Chunlan , Yu Yaohua 
Published: April 2023

Abstract
Background: Paravertebral block (PVB) has been increasingly popular for postoperative analgesia. However, a well-designed randomized study is needed to estimate the efficacy and safety of PVB using thoracoscope-assisted technique for early postoperative analgesia following video-assisted thoracoscopic lobectomy (VATS LOBECTOMY).
Methods: 106 patients scheduled for elective VATS LOBECTOMY were randomly assigned into two groups in a 1:1 ratio. Control group: receiving patient-controlled intravenous analgesia (PCIA) after surgery; PVB group: receiving thoracoscopic-guided multi-points PVB before the end of the surgery combined with PCIA following surgery. The primary endpoint was opioid consumption within 48 h after surgery.
Results: Compared with control group, sufentanil consumption was significantly lower in PVB group with longer duration of first time PCIA usage, less times of analgesic pump pressing and less rescue analgetic consumption. VAS scores at rest and during exercising were significantly lower in PVB group at all time points within the first 48h after surgery. PVB group was also associated with significantly higher total QoR-40 scores and lower incidence of analgesia related adverse events.
Conclusions: Thoracoscopic-guided PVB were effective and safe for early postoperative analgesia after VATS LOBECTOMY. Additionally, thoracoscope-assisted modality is technically easy, it should be recommended before the end of VATS LOBECTOMY.
Factors Associated with Health-Related Quality of Life Among Breast Cancer Survivors in the Saudi Arabia: Cross Sectional Study
Authors: Omar Mohammed TA , Dhwayan Nouf Al , Al-Karni Mohammed Ali Turki , Ajarim Dahish , Idreess May Jameel Nouri , Gwada Rehab FM 
Published: April 2023

Abstract

Purpose Assessment of health-related quality of life (HRQOL) is a major concern among breast cancer survivors. There is little knowledge about HRQO and its determinants among Saudi women with breast cancer (BC). This study aimed to describe HRQOL in Saudi women with breast cancer and explore the association of HRQO with sociodemographic and clinical variables. Methods A cross-sectional study involving 147 BC participants who fulfilled a 36-item short form health survey (SF-36) administered by the Medical Outcomes Study. An analysis of multivariable linear logistic regression was conducted to determine what factors are associated with HRQOL. Results For the physical component scale, general health scored the highest (55.41 ± 17.99) while role physical function scored the lowest (48.61 ± 22.51). The mean scores in the mental component were higher for social function (56.91 ± 20.84), while the lowest score was observed for energy and vitality (49.62 ± 20.84). Significant differences were observed in many SF-36 domains across sociodemographic and clinical variables. Older age, having had a mastectomy as well as comorbid conditions, and receiving chemotherapy and hormonal therapy were associated with decreased HRQOL. However, being physically active, employed, and having more education were associated with higher HRQOL. Conclusion HRQOL is generally poor among breast cancer survivors in Saudi Arabia, depending on a variety of factors. These findings highlight the need for routine QOL assessment. Furthermore, recognizing these predictors may play a key role in maximizing HRQOL for breast cancer participants.
Risk factors of esophagojejunal anastomotic leakage after total gastrectomy for gastric and Siewert type II/III esophagogastric cancer: a retrospective analysis from a tertiary hospital.
Authors: Liu Junjie , Fang Jiaming , Chen Yingliang , Chen Yonghe , He Jiasheng , Deng Zijian , Peng Junsheng , Lian Lei , Chen Shi 
Published: April 2023

Abstract

Background:To detect the risk factors associated with esophagojejunal anastomotic leakage (EJAL) after total gastrectomy for gastric and Siewert type II/III esophagogastric cancer and investigate the effect of laparoscopic anastomosis on EJAL.Methods:The data for 609 patients underwent Roux-en-Y esophagojejunostomy after total gastrectomy between March 2015 and March 2021 were reviewed. Univariate and multivariate analyses were performed to evaluate the risk factors. We adopted propensity score matching to compare the rate of the anastomotic leakage of the laparoscopic and open anastomosis group.Results:EJAL was observed in 48 (7.9%) of 609 patients. Univariate analysis revealed that gender, age, the number of comorbidities, postoperative serum albumin, tumor location, duration of operation were risk factors associated with EJAL. Patients who had the following factors including male, age ≥50 years, the number of comorbidities ≥2, postoperative serum albumin ＜35 g/L, tumor location was esophagogastric junction, duration of operation ≥260 min were more likely to develop EJAL than those who had not. Multivariate analysis revealed that the number of comorbidities (OR 3.214, 95% CI 1.092 – 9.463,p= 0.034) and duration of operation (OR 2.68, 95% CI 1.251 – 5.740,p= 0.011) were independent risk factors associated with EJAL. 161 patients received laparoscopic anastomosis and 448 patients received open anastomosis. The rates of anastomotic leakage in the laparoscopic and open anastomosis groups were comparable after propensity score matching (Laparoscopic 8.1% vs Open 10.6%,p= 0.565).Conclusions:More morbidities and prolonged operative duration were independently associated with EJAL after total gastrectomy for gastric and Siewert type II/III esophagogastric cancer. Laparoscopic anastomosis did not increase the risk of anastomotic leakage, compared with open anastomosis.
Surgical outcomes of isolated hydrocoeles and concurrent V-Y-Scrotoplasty for a compound penoscrotal fusion of under-5 years at Ho Teaching Hospital
Authors: Ali Mahamudu Ayamba , Maalman Raymond Saa-Eru , Oyortey Mawuenyo Attawa , Anyorigiya John Noah , Naabo Nuhu Noel , Kyei Mathew Yamoah 
Published: April 2023

Abstract
Purpose: Failure of reconstructive surgery and wound breakdown occur when scrotoplasty is combined with other multiple surgeries. Unfortunately, hydroceles are common whether isolated or associated with other conditions in penoscrotal malformations that may require surgical intervention during the reconstruction procedure.
Method: We retrospectively reviewed the surgical outcome of under-five year old children with isolated hydrocoeles or in combination with other inguinoscrotal pathologies operated at the Ho Teaching Hospital over a 12-month period.The diagnoses, procedure performed, complications and days of hospital stay were analysed and descriptively presented.
Results: Majority 17(85.0%) of the 20 patients reviewed were diagnosed with communicating and vaginal hydrocoele with surgeries been division of the patent processus or excision of tunica vaginalis respectively. Left-sided lesions were dominance for communicating hydrocoele. Hydrocoeles associated with undescended testes and the Penoscrotal fusion in 15.0% had orchidopexy and penoscrotal reconstruction. The frequent complication was wound infection.
Conclusion: Wound infection was not a side effect against combine PSF reconstruction and hydrocoele operation however, it appeared to be more severe.
Assessment of stream order and drainage pattern of flood prone areas of Ebonyi State Southeastern Nigeria
Authors: Ogbonna Jeremiah Nwankwagu , Okoro Okoro Enyinnaya , Ibe Henry. 
Published: April 2023

Abstract
This study assessed the stream order and drainage pattern of flood prone areas to determine the relationship between stream order and the magnitude of the flood in Ebonyi state. The river courses, different tributaries and the drainage network within Ebonyi State were identified using the Shuttle Rader Topographic Mission (SRTM) which generated the river network with the aid of Topographic analysis of Spatial Analyst on Hydrology. This SRTM activated the examination of the effects of stream order and drainage pattern on flood areas of Ebonyi State using the Digital Elevation Model (DEM) which proved that the drainage gets heavier down the slope as more volume of water were added to the traversing channel, hence the upsurge resulting in floods. The data was acquired from the field through Geometric Positioning. The LANDSAT data were equally downloaded from United State Geological Survey (USGS) Earth explorer for 2019 and that of the Thematic Mapper (TM) for 2009. The STRM collected its data from its sporadic available drainage encompassing tributaries and drainage network within Ebonyi State. The study revealed the need for an adequate adjustments of the stream order to accommodate more volume of water within the basin that will empty into the major consequent river down the slope avert flood. Therefore there is a need for urgent adjustments and constructions of a drainage system to accommodate more water volumes.
Carbon dioxide fluxes over grassland ecosystems in the Middle Tianshan region of China with eddy covariance method
Authors: Zhang Kun , Wang Yu , Mamtimin Ali , Liu Yongqiang , Gao Jiacheng , Aihaiti Ailiyaer , Wen Cong , Song Meiqi , Yang Fan , Zhou Chenglong , Huo Wen 
Published: April 2023

Abstract
Grassland ecosystems are an important component of global terrestrial ecosystems and play a critical role in the carbon cycle of ecosystems. It is important to study the Carbon dioxide (CO2) process in the Middle Tianshan grassland ecosystem, which can be regarded as a typical representative of the mountain grasslands in Xinjiang. Eddy Covariance (EC) was used to continuously observe Xinjiang’s Middle Tianshan grassland ecosystem during the 2018 growing season. The results showed significant daily and monthly variations in net ecosystem exchange (NEE), gross primary productivity (GPP), and ecosystem respiration (Reco). On a daily scale, there was a net uptake of CO2 during the day and a net release during the night. The grassland was a carbon sink from 6:00 to 18:00 and a carbon source for the rest of the day. At the monthly scale, June and July were carbon sinks, while the other months were carbon sources. The accumulated NEE, GPP, and Reco throughout the growing season were − 329.49 g C m− 2, 779.04 g C m− 2, and 449.55 g C m− 2, respectively. On the half-hourly and daily scales, soil temperature (Ts) contributed the most to CO2 fluxes and was the main factor influencing variations in CO2 fluxes, while air temperature (Ta) was also highly correlated with CO2 fluxes. CO2 uptake in grassland ecosystems was strongest and peaked at soil temperatures of 25°C. As the air temperature reaches above 15℃, NEE gradually decreases while CO2 uptake increases.
Native bioagents released phytohormones induce biochemical changes in rice against stem rot and false smut diseases
Authors: Sowmya V , Gopalan N. S. Raja , Maruthi P , Baskar M , Umadevi G , D Ladha lakshmi , P Koteshwar , G Jesudasu , Rathod Santhosha , M Srinivas Prasad , P Rajanikant , G Rekha , SK Hajira , RM Sundaram , Mohapatra Sridev , C Kannan 
Published: April 2023

Abstract

Rhizosphere essentially harbours native bioagents that are beneficial to plants growth and development, stress tolerance and competitive advantage over its rivals. The bioagents often exhibits multiple mechanisms including secretion of secondary metabolites, phytohormones and defense enzymes to offer such advantages to the host plants. Stem rot and false smut caused by Sclerotium hydrophilumand Ustilaginoidea virensrespectively, are two major constraints in India and other countries where rice is cultivated. There are reports on the critical role of microbial phytohormones viz., ABA and SA, IAA, GA, zeatin on growth and stress of plants. They play a role in the regulation of plant immune responses by eliciting induced systemic resistance (ISR) in host plants against invading pathogens. Accordingly, we studied the activity of native bioagents viz., Trichoderma asperellum strain TAIK 1, Bacillus cabrialesii strain BIK3, Pseudomonas putida strain PIK1 and Pseudomonas otitidis strain POPS1 on enhancement of plant growth, increase in defence enzymes and reduction in severity of stem rot and false smut diseases of rice. We followed the Liquid Chromatography Electrospray Ionization Tandem Mass Spectrometric (LC-ESI-MS/MS) method for the analysis of the phytohormones viz., IAA, GA, Zeatin, SA and ABA released by the bioagents. Bioagents treated plants showed 100% germination, enhanced plant growth parameters and reduction in percent disease index like 20.98% (stem rot), 23.14% (false smut) over the control plants (70.01% for stem rot and 75.24% for false smut). Our study indicated a direct relationship between the number and intensity of phytohormones released by the bioagents and the plant growth and disease suppression in rice.
Genetically predicted levels of circulating cytokines and risk of Alzheimer's and Parkinson's disease: evidence from complementary genetic methods
Authors: Zhang Kai , Wang Yani , Li Jiqing , Liu Xiaowen , Fu Ping , Xue Fuzhong 
Published: April 2023

Abstract
Background Alzheimer's disease (AD) and Parkinson's disease (PD) are the top two common neurodegenerative diseases. In recent years, the role of cytokines-related neuroinflammatory response in the pathogenesis of AD/PD has received increasing attention. This study aims to investigate the relationship between genetically determined cytokines and the risk of AD/PD.Methods Four systematic univariable Mendelian randomizations (MR) approaches were adopted to assess the causal effect of cytokines on AD/PD and their robustness, including inverse-variance weighted (IVW), weighted-median estimator (WME), robust adjusted profile score (RAPS), and wald ratio (WRO) methods. Furthermore, multivariate MR (MVMR) with Bayesian model averaging (MR-BMA) was performed to prioritize the cytokine combinations that may be on the AD/PD causal pathway. Besides, we used the standard IVW MVMR to re-estimate the causal effect and confidence interval of each cytokine in the top 10 models screened by MR-BMA. Bidirectional MR analysis was additionally performed to detect the causal directionality. Finally, results generated by univariate MR and MR-BMA methods were combined.Results Univariable MR (IVW) analysis indicated genetically determined higher levels of macrophage inflammatory protein-1 beta (MIP1b) were positively associated with both AD and PD [odds ratios (ORs) and 95% confidence intervals (95%CIs) were 1.05 (1.01–1.09) and 1.06 (1.02–1.10)]. Besides, Interleukin (IL) IL16 was positively associated with PD [OR (95%CI): 1.09 (1.01–1.18)]. However, IL2 receptor alpha (IL2ra) was negatively associated with AD [OR (95%CI): 0.92 (0.86–0.98)]. Particularly, MR-BMA analysis corroborated that MIP1b was the top priority risk factor for both AD and PD [posterior probability (marginal inclusion probability): 0.212 (0.190) and 0.509 (0.782)]. In addition, standard IVW MVMR detected that the genetical positive effect of MIP1b on both AD and PD was still significant. The relationship between MIP1b and PD was further replicated by FinnGen summary-level dataset. Finally, the results of bidirectional MR showed neither AD nor PD could modify the level of MIP1b.Conclusions Genetic predisposition to elevated circulating levels of MIP1b was associated with a higher risk of both AD and PD. In addition, MIP1b was screened as the most prioritized cytokine-related risk factor. Whether targeting MIP1b or its pathways could lower the AD/PD incidence requires future functional studies.
Provenance and pretreatment effect on seed germination of twenty-six provenances of Xanthoceras sorbifolium
Authors: Yang Yingying , Liu Xiaojuan , Zhao Ziquan , xu Huihui , Fu Guanghui , Bi Quanxin , Wang Libing 
Published: April 2023

Abstract

Due to the long dormancy period and hard seed coat, the natural germination percentage of the yellowhorn (Xanthoceras sorbifolium) seeds is very low, which seriously hinders the large-scale cultivation of the yellowhorn. In this study, the seeds of 26 provenances with different climatic and geographical conditions were collected and the seed germination was tested, which was conducted to determine the effects of seed pretreatment methods on the germination of yellowhorn at China from twenty-six provenances. Germination percentages (GP), mean germination time (MGT) and germination index (GI) were calculated and the data was subjected to ANOVA. The study showed that the GP, GI and MGT of twenty-six provenances of yellowhorn seeds in different pretreatment methods were highly significantly (P<0.01). Within the same provenance, the GP, GI and MGT using five pretreatment methods also had significant differences, except those of Jingbian, Fangshan, Kulun and Tubuxin provenances (P<0.05). In addition, the study indicates that provenances are as important as pretreatments in germination of the species. Correlation between germination ability and geo-climatic data of seed collection sites showed if the annual average temperature of the provenance with short frost-free period is low, the germination percentage of seeds will increase which pretreated with sand storage + PEG. This study provides a reference for the selection of suitable pretreatment methods for 26 provenances of yellowhorn.