bluuebunny/crossref_metadata_2025_split huggingface dataset:
Development of Empirical Models to Predict Gap Acceptance Parameter Based on the Geometrical and Operational Parameters of Different Roundabouts
Authors: Al Hasanat Haitham A. , Juhász János 
Published: January 2023

This paper develops a mathematical formula that accounts the influence of roundabout’s design and performance parameters to predict the gap acceptance parameter “Critical gap”. Thirteen roundabouts in Hungary having different geometric and operational parameters were selected. The geometrical and operational data of each roundabout’s leg was collected. Raff's method was used to estimate the critical gap for each roundabout leg. Firstly, the collinearity analysis was carried out to identify independent parameters to avoid any possible negative impact on the developed predictive models. Nine out of ten parameters passed the collinearity test. These nine parameters are the main parameters used in the model development. Three models were developed. The first model (M1) is based on Multivariate Adaptive Regression Spline (MARS) algorithm. The second model (M2) is based on Pearson correlation. The last model (M3) was based on Spearman correlation. Linear regression models were constructed using the retained parameters of the M2 and M3 models. Subsequently a comparison of the three developed models is done based on R2 and RMSE values. Based on the results obtained from the comparison, the MARS model (M1) is the best predictive model of the critical gap. According to the results of the MARS model, the most important parameters for predicting critical gap value are circulating traffic flow and the distance between neighboring legs.
Analysis of ecosystem resilience in Jiuzhaigou Valley Scenic Area under the effect of geohazards
Authors: Sui Haixia , Liu Chang , Ye Chengming , Xu Xiaolong , Sui Tianbo 
Published: January 2023

The effects of geohazards on the ecological environment and ecological spatial pattern have received wide attention from scholars. However, the positive role played by ecological restoration projects on the environment and in the reduction of geohazards has usually been neglected. Jiuzhaigou Valley Scenic Area is a world natural heritage area, has a high incidence of geohazards, and is a demonstration area for ecological restoration projects. Based on remote sensing technology, this paper adopted an InVEST model (Integrated Valuation of Ecosystem Services and Tradeoffs) and ecological landscape index to extract information about spatio-temporal changes in Jiuzhaigou from 2013 to 2020. This study utilized a quantitative analysis of the ecological recoverability of Jiuzhaigou in cases of artificial restoration and spontaneous restoration under different types of geohazards. Results showed that forests play a vital role in maintaining and controlling habitat quality; artificial restoration can significantly ameliorate the impact of geohazards on the scenic area. As of 2020, the forested scenic area recovered 3.868 km2, and the habitat quality index rebounded to 98.88% of the historical high. The ecological restoration project significantly shortened the scenic area recover time of its ecosystem service capability.
Present-day activity and seismic potential of the north Qinling fault, southern ordos block, central China, as revealed from GPS data and seismicity
Authors: Yang Chenyi , Ji Lingyun , Yang Yihai , Su Lina , Wang Ying , Shi Huiren 
Published: January 2023

The North Qinling Fault, located at the boundary of the North China Block and the South China Block, represents an important tectonic structure between the Weihe Basin and the Qinling Mountains, and controls the subsidence and expansion of the Weihe Basin. This fault has been highly active and has caused strong earthquakes since the Holocene and in a pre-seismic stage currently, as indicated by the many paleoearthquake traces found along it. To determine the present-day activity and seismic potential of the North Qinling Fault, by inverting GPS data, we produced fault locking depth, slip rate, and regional strain fields maps; moreover, based on seismicity, we produced a seismic b-value map. Combining this information with modern seismicity, we were able to comprehensively analyze the seismic potential of different fault segments. Our inversion of GPS data showed that the slip rate of the western segment of the fault (Qingjiangkou–Xitangyu) and the correspondent locking depth are 1.33 mm/a and 13.54 km, respectively, while the slip rate of the middle segment (Xitangyu–Fengyukou) and the correspondent locking depth are 0.45 mm/a and 8.58 km, respectively; finally, the slip rate of the eastern segment (Xitangyu–Daiyu) and the correspondent locking depth are 0.36 mm/a and 21.46 km, respectively. The locking depths of the western and middle segments of the fault are shallower than 90% of the seismic cutoff depth, while the locking depth of the eastern segment of the fault is similar to 90% of the seismic cutoff depth, indicating that “deep creep” occurs in the western and middle segments, while the eastern segment is locked. Modern small earthquakes have involved the western and middle segments of the fault, while the eastern segment has acted as a seismic gap with weak seismicity, characterized by a higher shear strain value and a lower b-value. These characteristics reflect the relationship between the locking depth and seismicity distribution. The results of our comprehensive analysis, combined with field geological surveys, show that the eastern segment of the North Qinling Fault has a strong seismic potential and is presently locked.
Overview of the application of ecological concrete in sponge city construction
Authors: Wang Lei , Liu Zhikui , Yan Yan , Li Shengdong , Xie Yongxiong 
Published: January 2023

As a prominent component of the construction field of sponge cities, ecological concrete is an essential tool to reach the goals of green, low-carbon living and sustainable development. A quantitative summary of the preliminary research on ecological concrete infrastructure in sponge city architecture is needed. Therefore, CiteSpace and VOSviewer were applied to perform a comparative analysis of the number of papers, countries, institutions, core authors, literature co-citations, research hotspots, and future trends in ecological concrete in the sponge city construction industry. The results show that the number of papers on ecological concrete is increasing, the research collaboration between domestic and foreign authors is relatively single, and there is insufficient interdisciplinary integration between institutions and the phenomenon of “relatively independent research.” The number of papers published in the field of ecological concrete construction has been on the rise, reaching more than 100 in each of the last 10 years, with China and the United States contributing more to the scientific output of the field. To meet the needs of global environmental protection and resource conservation, the theme of “promoting comprehensive resource conservation and recycling” will continue in the future, making concrete a feature of green, low-carbon, sustainable development and other areas of environmental protection in the construction field.
Controls on anomalously high porosity/permeability of Middle Jurassic deeply buried tight sandstones in the Taibei Sag, Turpan-Hami Basin, northwestern China: Implications for reservoir quality prediction
Authors: Wu Hao , Ban Shuyue , Du Zhiwei , Hao Aisheng , Li Jian , Wei Guoliang , Zhang Hua , Zhang Yunzhao , Wu Heng , Li Mi 
Published: January 2023

The lower member of the Middle Jurassic Xishanyao Formation (J2x1+2) sandstones are significant exploratory targets for hydrocarbon resources in the Taibei Sag of the Turpan-Hami Basin, northwestern China. Formation of anomalously high porosity/permeability in deeply buried J2x1+2 sandstones and reservoir quality prediction were investigated using a variety of petrographic analyses. These results show that the J2x1+2 sandstones are mostly feldspathic litharenites and litharenites, which are characterized by moderate-to good sorting, silty to medium granularity, and point- to long grain contacts. The J2x1+2 sandstone has low porosity (avg 6.0%) and permeability (avg 1.12 mD), but shows anomalously high porosity/permeability at depth interval of 3850–4050 m. There is a noticeable variation in pore types and sizes from intergranular-intragranular dissolution pores with a size mode of 2.0 μm in anomalously high porosity (AHP) sandstones, to major intragranular dissolution pores with a size mode of 0.5 μm in normally high porosity (NHP) sandstones, to most micropores with a size mode of 0.25 μm occurred in the normally low porosity (NLP) sandstones. The compaction is the main cause of porosity destruction, resulting in an average porosity loss of 89.6%. However, the authigenic minerals have relatively little influence on reservoir quality. The combination of nappe tectonics and well-sorted particles alleviated the compaction and thus preserved more intergranular pores in the AHP sandstones. Dissolution further increases the porosity and eventually forms the AHP sandstones. The strong carbonate-cemented facies (SCC), tightly compacted facies (TC), moderately compacted with moderate dissolution facies (MCMD), and weakly compacted with strong dissolution facies (WCSD) are determined in the J2x1+2 sandstones. The sandstones with SCC and TC are recognized as the NLP reservoirs. The MCMD and WCSD correspond to the NHP and AHP reservoirs, respectively. The reservoir quality predicted using the constructed diagenetic facies charts is in good agreement with the photomicrograph observations and physical property tests. Additionally, the AHP reservoirs are still developed in the deeply buried sandstones with depths larger than 4000 m.
Identification of ULK1 as a novel mitophagy-related gene in diabetic nephropathy
Authors: Yang Yuan-Yuan , Gao Zhong-Xiuzi , Mao Zi-Hui , Liu Dong-Wei , Liu Zhang-Suo , Wu Peng 
Published: January 2023

BackgroundAccumulating evidence indicates that mitophagy is crucial for the development of diabetic nephropathy (DN). However, little is known about the key genes involved. The present study is to identify the potential mitophagy-related genes (MRGs) in DN.MethodsFive datasets were obtained from the Gene Expression Omnibus (GEO) database and were split into the training and validation set. Then the differentially expressed MRGs were screened and further analyzed for GO and KEGG enrichment. Next, three algorithms (SVM-RFE, LASSO and RF) were used to identify hub genes. The ROC curves were plotted based on the hub genes. We then used the CIBERSORT algorithm to assess the infiltration of 22 types of immune cells and explore the correlation between hub genes and immune cells. Finally, the Nephroseq V5 tool was used to analyze the correlation between hub genes and GFR in DN patients.ResultsCompared with the tubulointerstitium, the expression of MRGs was more noticeably varied in the glomeruli. Twelve DE-MRGs were identified in glomerular samples, of which 11 genes were down-regulated and only MFN1 was up-regulated. GO and KEGG analysis indicated that several enrichment terms were associated with changes in autophagy. Three genes (MFN1, ULK1 and PARK2) were finally determined as potential hub genes by three algorithms. In the training set, the AUROC of MFN1, ULK1 and PARK2 were 0.839, 0.906 and 0.842. However, the results of the validation set demonstrated that MFN1 and PARK2 had no significant difference in distinguishing DN samples from healthy controls, while the AUROC of ULK1 was 0.894. Immune infiltration analysis using CIBERSORT showed that ULK1 was positively related to neutrophils, whereas negatively related to M1 and M2 macrophages. Finally, ULK1 was positively correlated with GFR in Nephroseq database.ConclusionsULK1 is a potential biomarker for DN and may influence the development of diabetic nephropathy by regulating mitophagy.
Observation study of using a small dose of rituximab treatment for thyroid-associated ophthalmopathy in seven Chinese patients: One pilot study
Authors: Wang Yueyue , Hu Hao , Chen Lu , Zhang Haitao , Yang Tao , Xu Xiaoquan , Chen Huanhuan 
Published: January 2023

ObjectiveTo report the efficacy, long-term safety, and tolerability of using a small dose (125 mg/m2 weekly for 4 weeks) of rituximab to treat Chinese patients with thyroid-associated ophthalmopathy (TAO).MethodsSeven patients with active moderate-to-severe TAO were prospectively recruited in this study. A small dose of rituximab (125mg/m2 body surface area) was given weekly with a duration of four weeks. Thyroid function, thyrotropin receptor antibody (TRAb), B cell and T cell subsets, ophthalmological examination, magnetic resonance imaging derived parameters, and adverse reactions were recorded at each visit.ResultsSeven patients were followed for an average of 224 weeks. B-cell depletion was observed in all patients following rituximab infusion. The clinical activity score (CAS) decreased from 4.86 ± 0.69 to 3.00 ± 0.82 at 5 weeks after treatment (P = 0.033) and remained significantly lower than baseline values at the end of follow-up (P = 0.001). Compared to baseline values, significant decreases in exophthalmos of the right eye, the thickness of extraocular muscles with maximum signal intensity, and the highest signal intensity ratio (SIR) of extraocular muscle to ipsilateral temporal muscle values were observed at the last follow-up (all P &lt; 0.05). Disease progressions or recurrences were not observed during follow-up. Only mild fatigue was observed after the first infusion as a side effect (n = 1).ConclusionSmall dose of rituximab may be a promising option with adequate safety, tolerability, and long-term efficacy for patients with active moderate-to-severe TAO.
Augmentation of IFN-γ by bone marrow derived immune cells in the presence of severe suppression of IFN-γ in gingivae induced by zoledronic acid and denosumab in Hu-BLT mice model of ONJ
Authors: Kaur Kawaljit , Sun Yujie , Kanayama Keiichi , Morinaga Kenzo , Hokugo Akishige , Nishimura Ichiro , Jewett Anahid 
Published: January 2023

IntroductionThe potential mechanisms governing drug induced osteonecrosis of the jaw (ONJ) is not well understood, and is one of the objectives of this study. Thus, we tested the release of IFN-γ within different immune compartments including bone marrow and gingivae upon treatment with zoledronic acid (ZOL) and denosumab which are known to induce ONJ in susceptible individuals.MethodsWe used humanized-BLT mouse model for the in-vivo studies reported in this paper. To determine the effects of zoledronic acid and denosumab on IFN-γ secretion and NK cell-mediated cytotoxicity; peripheral blood, bone marrow, spleen and gingiva were obtained after the injection of ZOL and denosumab in mice.ResultsPercentages of B cells are much higher in wild-type mice whereas the proportions of immune subsets in humans and reconstituted hu-BLT peripheral-blood are similar. Therefore, hu-BLT mice are preferable model to study human disease, in particular, immune-pathologies induced by ZOL and denosumab. Both agents resulted in a severe suppression of IFN-γ in the gingiva, whereas they heightened the release of IFN-γ and NK cell-mediated cytotoxicity by the BM-derived immune cells. ZOL increased the IFN-γ secretion by the spleen and peripheral blood immune cells, whereas denosumab decreased the release IFN-γ by these cells significantly.DiscussionZOL and denosumab may likely suppress IFN-γ secretion in gingiva through different mechanisms. In addition, to the suppression of IFN-γ secretion, denosumab mediated effect could in part be due to the decrease in the bone resorptive function of osteoclasts due to the induction of antibody dependent cellular cytotoxicity and lysis of osteoclasts, whereas ZOL is able to mediate cell death of osteoclasts directly. Suppression of IFN-gamma in gingiva is largely responsible for the inhibition of immune cell function, leading to dysregulated osteoblastic and osteoclastic activities. Restoration of IFN-gamma in the local microenvironment may result in establishment of homeostatic balance in the gingiva and prevention of osteonecrosis of jaw.
Estimated discharge of microplastics via urban stormwater during individual rain events
Authors: Ross Matthew S. , Loutan Alyssa , Groeneveld Tianna , Molenaar Danielle , Kroetch Kimberly , Bujaczek Taylor , Kolter Sheldon , Moon Sarah , Huynh Alan , Khayam Rosita , Franczak Brian C. , Camm Eric , Arnold Victoria I , Ruecker Norma J 
Published: January 2023

Urban stormwater runoff is an important pathway for the introduction of microplastics and other anthropogenic pollutants into aquatic environments. Highly variable concentrations of microplastics have been reported globally in runoff, but knowledge of key factors within urban environments contributing to this variability remains limited. Furthermore, few studies to date have quantitatively assessed the release of microplastics to receiving waters via runoff. The objectives of this study were to assess the influence of different catchment characteristics on the type and amount of microplastics in runoff and to provide an estimate of the quantity of microplastics discharged during rain events. Stormwater samples were collected during both dry periods (baseflow) and rain events from 15 locations throughout the city of Calgary, Canada’s fourth largest city. These catchments ranged in size and contained different types of predominant land use. Microplastics were found in all samples, with total concentrations ranging from 0.7 to 200.4 pcs/L (mean = 31.9 pcs/L). Fibers were the most prevalent morphology identified (47.7 ± 33.0%), and the greatest percentage of microplastics were found in the 125–250 µm size range (26.6 ± 22.9%) followed by the 37–125 µm size range (24.0 ± 22.3%). Particles were predominantly black (33.5 ± 33.8%), transparent (22.6 ± 31.3%), or blue (16.0 ± 21.6%). Total concentrations, dominant morphologies, and size distributions of microplastics differed between rain events and baseflow, with smaller particles and higher concentrations being found during rain events. Concentrations did not differ significantly amongst catchments with different land use types, but concentrations were positively correlated with maximum runoff flow rate, catchment size, and the percentage of impervious surface area within a catchment. Combining microplastic concentrations with hydrograph data collected during rain events, we estimated that individual outfalls discharged between 1.9 million to 9.6 billion microplastics to receiving waters per rain event. These results provide further evidence that urban stormwater runoff is a significant pathway for the introduction of microplastics into aquatic environments and suggests that mitigation strategies for microplastic pollution should focus on larger urbanized catchments.
Whole-genome DNA methylome analysis of different developmental stages of the entomopathogenic fungus Beauveria bassiana NCHU-157 by nanopore sequencing
Authors: Li Yi-Hsuan , Chang Ju-Chun , Yen Ming-Ren , Huang Yu-Feng , Chen Tzu-Han , Chen Li-Hung , Nai Yu-Shin 
Published: January 2023

The entomopathogenic fungus (EPF), Beauveria bassiana, is an important and commonly used EPF for microbial control. However, the role of DNA methylation has not been thoroughly studied. Therefore, the whole genomic DNA methylome of one promising EPF isolate, B. bassiana NCHU-157 (Bb-NCHU-157), was investigated by Oxford Nanopore Technologies (ONT). First, the whole genome of Bb-NCHU-157 was sequenced by next-generation sequencing (NGS) and ONT. The genome of Bb-NCHU-157 contains 16 contigs with 34.19 Mb and 50% GC content, which are composed of 10,848 putative protein-coding genes. Two putative DNA methyltransferases (DNMTs) were found, including Dim-2 and C-5 cytosine-specific DNA methylases. Both DNMTs showed higher expression levels in the mycelium stage than in the conidia stage, indicating that development of DNA methylation in Bb-NCHU-157 might occur in the mycelium stage. The global methylation level of the mycelium stage (5 mC = 4.56%, CG = 3.33%, CHG = 0.74%, CHH = 0.49%) was higher than that of the conidial stage (5 mC = 2.99%, CG = 1.99%, CHG = 0.63%, CHH = 0.37%) in both the gene and transposable element (TE) regions. Furthermore, the TE regions showed higher methylation frequencies than the gene regions, especially for CHH site methylation, suggesting regulation of genomic stabilization during mycelium development. In the gene regions, high methylation frequencies were found around the transcription start site (TSS) and transcription end site (TES). Moreover, CG and CHG methylation mainly occur in the promoter and intergenic regions, while CHH methylation occurs in the TE region. Among the methylated regions, 371, 661, and 756 differentially DNA methylated regions (DMRs) were hypermethylated in the mycelium in CG, CHG, and CHH, while only 13 and 7 DMRs were hypomethylated in the mycelium in CHG, and CHH, respectively. Genes located in the DMR shared the GO terms, DNA binding (GO: 0003677), and sequence-specific DNA binding (GO: 0043565) for hypermethylation in the mycelium, suggesting that methylation might regulate gene expression from the initial process. Evaluation of the DNA methylome in Bb-NCHU-157 by ONT provided new insight into this field. These data will be further validated, and epigenetic regulation during the development of B. bassiana will be explored.
Analysis of neutralizing antibodies to COVID-19 inactivated or subunit recombinant vaccines in hospitalized patients with liver dysfunction
Authors: Li Hu , Li Shiyin , Xu Pan , Wang Xiaohao , Deng Huan , Lei Yu , Zhong Shan 
Published: January 2023

BackgroundThe neutralizing antibodies (NAbs) response after COVID-19 vaccination after liver dysfunction is unclear. In this study, we evaluated the NAbs response after COVID-19 vaccination in hospitalized patients suffering from liver dysfunction.MethodsIn this cross-sectional study with longitudinal follow-up, we enrolled eligible patients with liver dysfunction and healthy volunteers with full-course COVID-19 vaccination. Blood samples were collected for the NAbs testing at the time of admission and after treatment. Multiple regression analysis to assess independent risk factors affecting NAbs response.ResultsA total of 137 patients and 134 healthy controls (HC) were enrolled. Both seropositivity (65.7% vs 80.6%, p&lt;0.01) and titer (3.95 vs 4.94 log2 AU/ml, p&lt;0.001) of NAbs in patients were significantly lower than that in HC. The decrease of antibody titer in patients was significantly faster than that in HC. After adjusting for potential confounding factors, males (odds ratio [OR]: 0.17; 95% confidence interval [CI]: 0.06, 0.46; p&lt;0.001) and severe liver damage (OR: 0.30; 95% CI: 0.12, 0.71; p&lt;0.01) were significantly associated with reduction of the probability of NAbs seropositivity in the multiple regression analysis. Males (β =-1.18; 95% CI: -1.73,-0.64) and chronic liver diseases (β =-1.45; 95% CI: -2.13, -0.76) were significantly associated with lower NAbs titers. In 26 patients with liver failure, both antibody seropositivity (53.8% vs 84.6%, p&lt;0.05) and titer (3.55 vs 4.32 log2 AU/ml, p&lt;0.001) did not decrease but increased after artificial liver plasmapheresis.ConclusionsNAbs response to COVID-19 inactivated or subunit recombinant vaccines was waning in patients with liver dysfunction. Moreover, patients with male sex, severe liver injury and chronic liver diseases have an increased risk of poor antibody responses.