bluuebunny/crossref_metadata_2025_split huggingface dataset:
Functional analysis of a putative HER2-associated expressed enhancer, Her2- Enhancer1, in breast cancer cells
Authors: Rojhannezhad Mahdieh , Soltani Bahram M. , Vasei Mohammad , Ghorbanmehr Nassim , Mowla Seyed Javad 
Published: January 2023

Abstract
HER-2/neu (HER2) is a member of epidermal growth factor receptors (EGFR) family, encoding a protein with tyrosine kinase activity. Following the gene amplification or increased HER2 transcription, carcinogenesis has been observed in some cancers. Genetic and epigenetic changes occurring in enhancer sequences can deeply affect the expression and transcriptional regulation of downstream genes, which can cause some physiological and pathological changes, including tumor progression. A therapeutic approach that directly targets the genomic sequence alterations is of high importance, with low side effects on healthy cells. Here, we employed the CRISPR/Cas9 method to genetically knockout an expressed putative enhancer (GH17J039694; we coined it as Her2-Enhancer1) located within the HER2 gene, 17q12: 39694339–39697219 (UCSC-hg38). We then investigated the potential regulatory effect of Her2-Enhancer1 on HER2 and HER2-interacting genes. In order to evaluate the cis and trans effects of Her2-Enhancer1, genetic manipulation of this region was performed in HER2-positive and -negative breast cancer cells. Our bioinformatics and real-time PCR data revealed that this putative enhancer region is indeed expressed, and act as an expressed enhancer. Further functional analysis on edited and unedited cells revealed a significant alteration in the expression of HER2 variants, as well as some other target genes of HER2. Moreover, apoptosis rate was considerably elevated within the edited cells. As we expected, Western blot analysis confirmed a reduction in protein levels of HER2, GRB7, the gene interacting with HER2, and P-AKT in PI3K/AKT pathway. Altogether, our findings revealed an enhancer regulatory role for Her2-Enhancer1 on HER2 and HER2-interacting genes; and that this region has a potential for targeted therapy of HER2-positive cancers.
Automatic stridor detection using small training set via patch-wise few-shot learning for diagnosis of multiple system atrophy
Authors: Ahn Jong Hyeon , Lee Ju Hwan , Lim Chae Yeon , Joo Eun Yeon , Youn Jinyoung , Chung Myung Jin , Cho Jin Whan , Kim Kyungsu 
Published: January 2023

Abstract
Stridor is a rare but important non-motor symptom that can support the diagnosis and prediction of worse prognosis in multiple system atrophy. Recording sounds generated during sleep by video-polysomnography is recommended for detecting stridor, but the analysis is labor intensive and time consuming. A method for automatic stridor detection should be developed using technologies such as artificial intelligence (AI) or machine learning. However, the rarity of stridor hinders the collection of sufficient data from diverse patients. Therefore, an AI method with high diagnostic performance should be devised to address this limitation. We propose an AI method for detecting patients with stridor by combining audio splitting and reintegration with few-shot learning for diagnosis. We used video-polysomnography data from patients with stridor (19 patients with multiple system atrophy) and without stridor (28 patients with parkinsonism and 18 patients with sleep disorders). To the best of our knowledge, this is the first study to propose a method for stridor detection and attempt the validation of few-shot learning to process medical audio signals. Even with a small training set, a substantial improvement was achieved for stridor detection, confirming the clinical utility of our method compared with similar developments. The proposed method achieved a detection accuracy above 95% using data from only eight patients with stridor for training. Performance improvements of 4%--13% were achieved compared with a state-of-the-art AI baseline. Moreover, our method determined whether a patient had stridor and performed real-time localization of the corresponding audio patches, thus providing physicians with support for interpreting and efficiently employing the results of this method.
Boron Nitride-enabled Printing of Highly Sensitive and Flexible Iontronic Pressure Sensing System toward Spatial Mapping
Authors: Gao Libo , Yang Zekun , Duan Qikai , Zang Junbin , Zhao Yunlong , Zheng Weihao , Xiao Ran , Zhang Zhidong , Hu Liangwei , Wu Guirong , Nan Xueli , Xue Chenyang 
Published: January 2023

Abstract
Flexible iontronic pressure sensors (FIPS) with high sensitivity and wide sensing range compared to conventional capacitive sensors have recently been widely investigated. However, mainly due to the difficulty of fabricating nanostructures commonly used on electrodes or ionic layers by screen printing techniques, strategies to fabricate such devices using screen printing techniques to drive their mass production have been rarely reported. Here, for the first time, we employed the 2-dimensional (2D) material and hexagonal boron nitride (h-BN) as an additive and ionic liquid reservoir into the ionic film, which not only can make it printable but also significantly improve the sensor’s sensitivity and sensing range through screen printing. The engineered sensor exhibited high sensitivity (Smin> 261.4 kPa− 1) and broad sensing range (0.05–450 kPa) and is capable of stable operation at high pressure (400 kPa) for more than 5000 cycles. In addition, the integrated sensor array system allows accurate monitoring of wrist pressure and shows great potential for healthcare systems. We believe the concept of using h-BN as an additive into the ionic material for screen-printed FIPS would greatly inspire other research using 2D materials for similar such systems and other types of sensors.
Wilms tumor 1 associated protein promotes metastasis and chemo-resistance to oxaliplatin by nuclear factor kappa B pathway in gastric cancer
Authors: Liu Yu , Li Weidong , Yang Jian , Ma Weijun , Fan Chuanlei , Da Mingxu 
Published: January 2023

Abstract
Background
There is now substantial evidence that epigenetic modifications largely contribute to gastric carcinogenesis and metastasis, causing a large number of tumor-related deaths. N6-methyladenosine(m6A) is a modification that adds a methyl group at the N6 position, and Wilms tumor 1 associated protein (WTAP) is an important methylase of m6A. WTAP has been reported to play a role in a variety of tumors.
Results
In the present study, we found that WTAP was highly expressed in gastric cancer (GC) tissues and cells and promoted migration/invasion of GC cells. WTAP promoted the expression of B-cell lymphoma-2(bcl-2), inhibited the expression of Bax, increased the protein levels of p-IKKα, p65, p-p65 and p-IκBα, promoted the expression of nuclear factor kappa B (NF-κB) pathway, and mediated the resistance of GC cells to the chemotherapeutic drug oxaliplatin (OPX). Interestingly, the NF-κB pathway-specific inhibitor caffeic acid phenethyl ester (CAPE) eliminated OPX resistance generated by WTAP overexpression cells and essentially reduced it to the level of resistance in their control cells.
Conclusions
In summary, the WTAP/NF-κB/bcl-2 pathway may be critical in promoting GC cell proliferation and OPX resistance development, providing a potential therapeutic target for GC treatment.
An in-Silico Study of the Distribution of Mn Oxidation Proteins in Sequenced Bacterial Genomes
Authors: Kurdi M. Zakaria , Olichney Jacob , Geszvain Kati 
Published: January 2023

Abstract
While many species of bacteria have been identified that can convert soluble, reduced manganese (Mn+2) into insoluble, oxidized Mn+4 oxides, the mechanisms these bacteria employ and their distribution throughout the bacterial domain are less well understood. Laboratory-based studies identified three Mn oxidase enzymes in Pseudomonas putida GB-1 (PpMnxG, McoA and MopA), one in Bacillus sp. PL-12 (BaMnxG), and one in Leptothrix discophora sp SS-1 (MofA).
By querying public databases of bacterial genome sequences for homologs to these Mn oxidase proteins, it was possible to determine the distribution of these proteins within bacteria. Most homologs were found in just three phyla: proteobacteria, actinobacteria and firmicutes. Each of the homologs had a statistically significant probability of being present as the solo Mn oxidase in a genome. When genomes did have more than one oxidase, they were present in the same combinations as in P. putida GB-1. These results do not support the initial hypothesis that multiple enzymes are required to complete the two-electron oxidation of Mn+2 to Mn+4. Alternatively, the various Mn oxidase enzymes may be optimized to function under different environmental conditions; organisms like P. putida GB-1 may need to oxidize Mn at different temperatures, nutritional states or oxygen conditions.
Time to Bridge the Gap between Accounting and Technology through Improving the Accounting Curriculum: A Perceptional View
Authors: Divyashree M S , N Abhishek , Kulal Abhinandan , Kanchan Swarn G 
Published: January 2023

Abstract
Today there are many advancements in accounting practices across the globe. But the accounting curriculum in the higher education institutions of India are still out dated. This in turn indirectly impacting on the transversal competencies of accounting graduates. Therefore, in the present study the opinions from academicians and professionals are gathered on the cuurent status of accounting education in India and the collected opinions are analyzed through independent samples t-test and concluded that higher education institutions in India needs to redesign accounting curriculum in par with professional educational curriculum like professionals education curriculum followed by professional institutes such as ICAI, ICSI, ICWA and IIMs at large.
The effect of health ambassadors’ training based on social cognitive theory on brucellosis preventive behaviors in rural areas: Randomized controlled trial
Authors: Khosraviasl Tahereh , Moshki Mahdi , Dogonchi Mitra 
Published: January 2023

Abstract
Background: Brucellosis is still a serious public health threat and an endemic disease in Iran. Despite eradication programs, it is a major challenging issue for human health. This study examined the effect of health ambassadors’ training based on social cognitive theory on brucellosis preventive behaviors in rural areas.
Methods: This study was conducted as a randomized controlled trial. The intervention program was performed in two steps. Step 1: Training of health ambassadors by the researcher and Step 2: Training of village residents by health ambassadors.120 residents of rural areas of Mashhad were selected from two villages using two-stage random sampling and were assigned to two intervention and control groups.
Results: The mean age of the participants in the intervention group was 33.9±90.97 years. There was a statistically significant difference between the mean scores of constructs of knowledge, outcome expectations, outcome value, self-efficacy, self-efficacy in overcoming obstacles, self-regulation, emotional adjustment, and behavior measurement after the intervention compared to before intervention(p <0.0001).
Conclusion: The results indicated that training programs based on SCT can be effective in improving brucellosis preventive behaviors. The training interventions that are designed to educate health ambassadors and villagers using social cognitive theory with a focus on individual, cognitive, environmental, and behavioral factors can improve behaviors that prevent brucellosis.
Trial registration:
This trial has been registered by the title of “The effect of health ambassadors’ training based on social cognitive theory on brucellosis preventive behaviors in rural areas: a randomized control trial” in the Iranian Registry of Clinical Trials (IRCT) with code “I IRCT20220204053935N1”, https://www.irct.ir/trial/47529. The registration date is May 18, 2022.
Data-driven adjoint-based calibration of port-Hamiltonian systems in time domain
Authors: Günther Michael , Jacob Birgit , Totzeck Claudia 
Published: January 2023

Abstract
We present a gradient-based calibration algorithm to identify the system matrices of a linear port-Hamiltonian system from given input-output time data. Aiming for a direct structure-preserving approach, we employ techniques from optimal control with ordinary differential equations and define a constrained optimization problem. The input-to-state stability is discussed which is the key step towards the existence of optimal controls. Further, we derive the first-order optimality system taking into account the port-Hamiltonian structure. Indeed, the proposed method preserves the skew-symmetry and positive (semi)-definiteness of the system matrices throughout the optimization iterations. Numerical results with perturbed and unperturbed synthetic data, as well as an example from the PHS benchmark collection demonstrate the feasibility of the approach.
AMS classification: 37J06, 37M99, 49J15, 49K15, 49M29, 49Q12, 65P10, 93A30, 93B30, 93C05
Paradoxical contralateral hemiparesis in spontaneous spinal epidural hematoma: A case report
Authors: Okada Kazuhiro , Fujita Youshi , Kitai Ryuhei 
Published: January 2023

Abstract
Background: Hemiparesis associated with spontaneous spinal epidural hematoma (SSEH) usually occurs ipsilateral to the hematoma. We here report the case of a patient with paradoxical hemiparesis contralateral to a spinal lesion due to SSEH.
Case presentation: A 70-year-old woman was identified in routine clinical practice; she presented with acute-onset neck pain and left hemiparesis. Neurological examination showed left-sided sensory-motor hemiparesis without facial involvement. Cervical MRI showed a dorsolateral epidural hematoma compressing the spinal cord at the C2 to C3 level. Axial imaging demonstrated a crescent hematoma on the right side, which is contralateral to the hemiparesis, and lateral displacement of the spinal cord. Spinal angiography revealed no abnormal vessels. Based on clinical presentation and MRI findings, a diagnosis of SSEH was made. The patient was managed conservatively. The symptoms completely resolved without any neurological deficits, and the hematoma disappeared on the follow-up MRI.
Conclusions: Paradoxical contralateral hemiparesis is one of the possible presenting symptoms in patients with SSEH. This case demonstrates the existence of the paradoxical contralateral hemiparesis associated with spinal compressive lesions. A plausible mechanism of the phenomenon is discussed.
Oncogenic HJURP is driven by P53/ E2F1/FOXM1-axis regulated enhancer and potentiates TNBC proliferation and invasion
Authors: jia yunlu , Chen Yongxia , Chen Ming , Zhou Jianbiao , Chng Wee-Joo , Xie Mixue , Jiang Qi , Xiong Hanchu , Ruan Jian , Wang Linbo , Shen Peng 
Published: January 2023

Abstract
Triple-negative breast cancer (TNBC) is the most aggressive subtype of breast cancer with poor outcomes and lacks effective targeted therapies. We utilized the epigenomic landscape, TCGA database and clinical samples to show the activation of HJURP in TNBC, which is associated with poor prognosis, metastasis, and advanced stage. RNA-seq analysis of HJURP silencing induced malignant phenotypes-related transcriptional signatures of TNBC. Specifically, knock-down of HJURP suppressed cell proliferation, migration, invasion, EMT progress, and induced apoptosis of TNBC. Analysis of publicly available data sets revealed that HJURP is elevated in mutP53 vs. wtP53 breast cancer cells. Inactivation of wild type P53, by loss or mutation of wtP53, increased HJURP expression, whereas accumulation of wild-type P53 reduced HJURP promoter activity and HJURP transcription. We found the activation of HJURP in TNBC was driven by the mutant P53-regulated enhancer instead of genetic alteration. P53 positively regulated the expression of transcription factor FOXM1 and E2F1, and the FOXM1/E2F1/H3K27ac complex preferentially occupied the HJURP-enhancer and regulated HJURP transcription by binding to the active elements. CRISPR interference of enhancer structure or specific disruption of enhancer complex inhibited HJURP transcription and phenocopied HJURP silencing, leading to impaired E2F1, FOXM1 and H3K27ac binding affinity. Consistent with this result, knock-down of FOXM1 or E2F1 reduced HJURP expression in TNBC cells containing mutant alleles of P53 gene. Lastly, we uncovered marked decreases in survival of breast cancer patients expressing high HJURP levels carrying wtP53. Our findings identify enhancer-driven HJURP as a molecular bypass that suppresses the anti-proliferative and pro-apoptotic effects exerted by wtP53. Targeting HJURP allows for effective suppression of tumor invasion and attenuating metastasis in P53-mutant TNBC.
Segmentation of Lung Lobes and Lesions in Chest CT for the Classification of COVID-19 Severity
Authors: Khomduean Prachaya , Phuaudomcharoen Pongpat , Boonchu Totsaporn , Taetragool Unchalisa , Chamchoy Kamonwan , Wimolsiri Nat , Jarrusrojwuttikul Tanadul , Chuajak Ammarut , Techavipoo Udomchai , Tweeatsani Numfon 
Published: January 2023

Abstract
To precisely determine the severity of COVID-19-related pneumonia, computed tomography (CT) is an imaging modality beneficial for patient monitoring and therapy planning. Thus, we aimed to develop a deep learning-based image segmentation model to automatically assess lung lesions related to COVID-19 infection and calculate the total severity score (TSS). The entire dataset consists of 100 COVID-19 patients acquired from Chulabhorn Hospital, divided into 25 cases without lung lesions and 75 cases with lung lesions categorized severity by radiologists regarding TSS. The model combines a 3D-UNet with pre-trained DenseNet and ResNet models for lung lobe segmentation and calculation of the percentage of lung involvement related to COVID-19 infection as well as TSS measured by the Dice similarity coefficient (DSC). Our final model, consisting of 3D-UNet integrated with DenseNet169, achieved segmentation of lung lobes and lesions with Dice similarity coefficients of 0.929 and 0.842, respectively. The calculated TSSs are similar to those evaluated by radiologists, with an R2 of 0.833. The correlation between the ground-truth TSS and model prediction was greater than that of the radiologist, which was 0.993 and 0.836, respectively.
Intelligent Assembly Method of The Profiled Thermal Battery Pack Based on Improved DE Algorithm
Authors: He Lin , Zhou Yingyu , Zhong Jiangchuan , Liu Dan 
Published: January 2023

Abstract
An intelligent assembly method was designed to realize the intelligent assembly of the profiled thermal battery pack and improve its assembly accuracy. Firstly, for the number and size of different monomer batteries vary, this paper takes the monomer battery thermal battery assembly as the object, with a common shape circle assembly screw arrangement and an established process model. Then, the assembly also has an improved differential evolution algorithm for assembly arrangement and process, on the number, location, tightening of the screw assembly, torque, and the order of solution. According to this scheme, the assembly and the flatness test were carried out. Results showed that the bottom plate of the assembly frame was “concave in the middle and warped around,” and the flatness error was large. The scheme was optimized by numerical simulation analysis. After optimization, the average offset of the floor plane was 0.04 mm, the offset accounted for 0.028% of the overall height, the maximum offset was 0.094 mm, and the offset was reduced by 0.312%.
Differences in carbon sink capacity and carbon storage of several typical modified tidal wetlands in the Yangtze River Estuary
Authors: Jialu Zhang , Haoyu Dong , Lupei Wang , Liwei Qian , Daming Lv , Weiguo Tao , Xiaofeng Qian , Xiaohua Fu , Lei Wang 
Published: January 2023

Abstract
Although tidal wetlands in the Yangtze River Estuary are important carbon sinks, some are modified by different engineering measures to protect beaches, prevent corrosion, and promote tidal flat siltation. Nevertheless, some basic characteristics of these modified wetlands are still retained for their ecological service functions. Elucidation of the differences in carbon sink capacity and carbon storage of different types of modified tidal wetlands and the underlying reasons can provide theoretical guidance and a scientific basis for the protection and rational utilization of tidal wetlands, so as to maintain and enhance their carbon sink capacity. In this study, five typical modified tidal wetlands in the Yangtze River Estuary, namely, enclosure coastal wetland with Spartina alterniflora (ECS), enclosure coastal wetland with Phragmites communis (ECP), siltation coastal wetland with S. alterniflora (SCS), rock rip-rap riverside wetland with P. communis (RRP), and reclamation riverside wetland with P. communis (ReRP), were selected as study zones, and their carbon sink capacity and carbon storage were evaluated by analyzing soil organic carbon density, plant carbon fixation, and soil CO2 emissions. The possible reasons for the differences among these modified wetlands were considered based on elevation, average waterlogging time, tidal salt content, and vegetation types. The results showed that all the modified wetlands were net carbon sinks, with SCS exhibiting the highest carbon sink capacity (8.34–8.52 kg CO2 m− 2 year− 1), reaching the level of natural wetland, owing to its higher plant biomass and lower soil respiration intensity, followed by ReRP (2.68–3.15 kg CO2 m− 2 year− 1), ECP (2.56–3.37 kg CO2 m− 2 year− 1), RRP (2.94–3.04 kg CO2 m− 2 year− 1), and ECS (1.24–1.86 kg CO2 m− 2 year− 1). ReRP (26.89–27.82 kg CO2 m− 2) with long carbon accumulation history and SCS (24.57–28.6 kg CO2 m− 2) with high annual carbon sink capacity presented higher carbon storage (< 40 cm), followed by RRP (22.52–24.97 kg CO2 m− 2), ECP (16.14–18.18 kg CO2 m− 2), and ECS (5.74–6.70 kg CO2 m− 2). The difference in waterlogging time caused by diverse elevation, salinity, and vegetation types significantly affected soil respiration and plant carbon fixation, thus impacting CO2 emission and carbon sink capacity of different modified tidal wetlands. `
Robot-assisted total hip arthroplasty is not associated with serious invasiveness
Authors: Ma Mingyang , Zhang Shuai , Cao Zheng , Yi Jiafeng , Kong Xiangpeng , Chai Wei 
Published: January 2023

Abstract
Background: Robot-assisted total hip arthroplasty (R-THA) is increasingly being performed throughout the world. The invasiveness of this operation is unknown.
Methods: Data from patients who received primary R-THA or manual THA (M-THA) for osteonecrosis of the femoral head between January 2020 and January 2022 were retrospectively reviewed. Preoperatively and on postoperative days 1 and 3, we compared the platelet (PLT), white blood cell (WBC), neutrophil rate, lymphocyte rate, erythrocyte sedimentation rate (ESR), C-reactive protein (CRP), interleukin-6 (IL-6), delta hemoglobin drop (delta Hb),creatine kinase (CK), lactate dehydrogenase (LDH), and alkaline phosphatase (ALP) levels between the groups. Age, gender, the pre- and post-operative Harris Hip Score (HHS), operating time, transfusion rate, the post-operative Forgotten Joint Score (FJS), and 12-week postoperative complications rate were all recorded.
Results: The PLT count was significantly lower, and the lymphocyte rate was significantly higher on the postoperative day 1, in the R-THA than M-THA group (both p < 0.05). There was no significant difference in the other serum markers between the groups on postoperative day 1 and 3 (p > 0.05). The operating time was significantly longer in the R-THA than M-THA group (p < 0.01). The FJS was significantly higher in the R-THA than M-THA group (p = 0.01). There was no significant difference in the postoperative HHS or complication rate between the groups (p > 0.05).
Conclusion: The R-THA is not associated with a serious invasiveness compared to M-THA, despite with longer operating time. Patients who underwent R-THA had a better early function compared to those who underwent M-THA.