bluuebunny/crossref_metadata_2025_split huggingface dataset:
dSTORM‐Based Single‐Cell Protein Quantitative Analysis Can Effectively Evaluate the Degradation Ability of PROTACs
Authors: Yang Fuwei , Tan Yuxin , Wu Cheng , Xin Lilan , Huang Zhenli , Zhou Haibing , Zhou Fuling 
Published: December 2022

AbstractAs an emerging therapeutic strategy, proteolysis‐targeting chimeras (PROTACs) have been proven to be superior to traditional drugs in many aspects. However, due to their unique mechanism of action, existing methods for evaluating the degradation still have many limitations, which seriously restricts the development of PROTACs. In this methodological study, using direct stochastic optical reconstruction microscopy (dSTORM)‐based single‐cell protein quantitative analysis, we systematically investigated the dynamic degradation characteristics of FLT3 protein during PROTACs treatment. We found that the distribution of FLT3 varies between FLT3‐ITD mutation and FLT3‐WT cells. PROTACs had an obvious time‐course effect on protein degradation and present two distinct phases; this provided a basis for deciding when to evaluate protein degradation. High concentrations of PROTACs were more effective than long‐time administration because a higher Dmax was achieved. Two‐color dSTORM‐based colocalization analysis efficiently detected the proportion of ternary complexes, making it very useful in screening PROTACs. Taken together, our findings show that the dSTORM method is an ideal tool for evaluating PROTACs and will accelerate the development of new PROTACs.
Scalable Photovoltaic‐Electrochemical Cells for Hydrogen Production from Water ‐ Recent Advances
Authors: Lee Minoh , Haas Stefan , Smirnov Vladimir , Merdzhanova Tsvetelina , Rau Uwe 
Published: December 2022

AbstractHydrogen is regarded as a very important pillar for the future energy supply because it is readily available from water and can be used for environmentally friendly electricity generation. Hydrogen can be produced in various ways. Water splitting powered by renewable resources (e. g., solar, wind, etc.) can be an ideal way of hydrogen generation in the future since this approach can achieve true net‐zero carbon dioxide emissions. This review article is aimed at giving an overview of the state‐of‐the‐art hydrogen generation driven by photovoltaics (PVs) on a relatively large‐scale (with PV area >50 cm2). The basic knowledge/principle of (PV‐driven) water splitting is introduced in the beginning part. Then, different types of PV‐driven water splitting devices and the recent advances in scalable PV‐electrochemical water splitting devices are intensively reviewed in the middle part. Finally, cost predictions and challenges that need to be addressed are presented at the end of this article.
Development of a Tyrosinase Amperometric Biosensor Based on Carbon Nanomaterials for the Detection of Phenolic Pollutants in Diverse Environments
Authors: Liu Yanran , Chen Yafei , Fan Yining , Gao Guanyue , Zhi Jinfang 
Published: December 2022

AbstractPhenolic compounds are widespread water contaminants with high toxicity and chronic carcinogenicity, whose concentration is restricted by a variety of water quality regulations. In the present study, we developed a tyrosinase biosensor based on a nanocomposite of carbon nanotubes, nanodiamonds, and soluble starch to enhance sensing performance during practical application. Doping nanodiamonds in soluble starch considerably improved the electrode‘s biocompatibility and electroactive sites, while carbon nanotubes improved the loading capacity and electron transfer efficiency of tyrosinase. The as‐prepared biosensor exhibited a broad detection range from 0.01 μM to 50 μM for phenol, which covered the concentration thresholds of the water standard requirement between drinking water to industrial effluents, and the limit of detection (LOD) is down to 2.9 nM. Furthermore, a portable instrument with an integrated probe and self‐calibrated algorithm software was developed to automatically detect and readout the phenol concentration. The as‐prepared instrument demonstrated good agreement to the ISO 4‐Aminoantipyrine spectrometric method in the phenol detection of wastewaters that was collected from a coal factory, chemical and biological manufacturing plants, and biological fermentation factory. The application of this novel biosensor and instrument are paving the way to a wider application of amperometric biosensors for in‐field phenol monitoring.
Chiral recognition of tryptophan enantiomer based on the electrode modified by polyaniline adsorption bovine serum albumin complex
Authors: Yao Wenyan , Li Sha , Xie Licheng , Jiang Yan 
Published: December 2022

AbstractA chiral sensing platform was constructed via adsorptive functionalization of ammonium persulfate doped polyaniline (APS‐DPANI) with bovine serum albumin (BSA). The novelty of this work is the construction of such chiral interface with adsorption principle. The material has been characterized by scanning electron microscope, Fourier transform infrared and X‐ray photoelectron spectroscopy, and thermogravimetric and water contact angle analyses. It displayed considerable stability in multi‐run cyclic voltammetric scanning. Moreover, the superior conductivity of APS‐DPANI and the decent binding ability of BSA endowed this sensing platform with an excellent recognition effect for tryptophan (Trp) enantiomers in the differential pulse voltammetry (DPV) test. The recognition was highly reproducible, and the detection limits for L‐ and D‐isomer were 0.071 and 0.0478 mM, respectively.
Mission alignment with employees and financiers: Probing into the workings of B Corp certification
Authors: Paelman Valerie , Van Cauwenberge Philippe , Vander Bauwhede Heidi 
Published: December 2022

AbstractSustainable enterprises are becoming more popular and play an important role in tackling environmental and social challenges. Their hybrid nature, that is, the combination of financial and societal goals, makes them prone to external legitimacy issues and competing internal logics, which results in mission drift. We investigate whether and how B Corp certification helps remedy mission drift by conducting semi‐structured interviews with 16 key employees in 13 Dutch and Belgian certified B Corps from various industries. Our findings show that B Corp certification helps ingrain the mission into human resource management, in both the recruitment and employee engagement processes, which contributes to internal alignment and lowers the risk of mission drift. In addition, certification helps articulate the company's dual nature to finance providers and find financing sources that relate more with the mission.
Usefulness of serum androgen isotope ratio mass spectrometry (IRMS) to detect testosterone supplementation in women
Authors: Andersson Alexander , Piper Thomas , Ekström Lena , Hirschberg Angelica Lindén , Thevis Mario 
Published: December 2022

AbstractThe detection of testosterone intake is facilitated by monitoring the urinary steroid profile in the athlete biological passport. This technique can be used with confidence to identify target samples for isotope ratio mass spectrometry. Regrettably, most research has been performed on male subjects resulting in a method that does not account for females' steroid concentration and/or variation. This study evaluates the usefulness of the carbon isotope ratio (CIR) in serum of female subjects. Two steroid sulphates are targeted in serum, androsterone and epiandrosterone. Both exhibit statistically significant depletion of their CIR after 10 weeks of daily (10 mg) transdermal testosterone administration. Of the 21 female subjects, samples from six individuals were identified as adverse analytical findings; additionally, four were found atypical considering the serum CIR. The urinary athlete biological passport was not sufficiently sensitive to identify target serum samples for isotope ratio mass spectroscopy. Of the six with a suspicious passport, only two could be confirmed using the serum CIR of androsterone and epiandrosterone. This study shows that CIR analysis in serum cannot be considered the sole confirmatory solution to detect testosterone doping in women due to low sensitivity. However, this analysis has the potential to be used as a complementary method in certain situations to confirm exogenous testosterone in women.
Social‐ecological cascade effects of land use on vertebrate pest dynamics in arid agricultural communities
Authors: Lewin Amir , Erinjery Joseph J. , Nissim Doron , Iwamura Takuya 
Published: December 2022

AbstractExtensive land conversion to agriculture in drylands and associated resource use have wide‐ranging impacts on desert ecosystems globally. Incorporating the impacts of human‐social aspects is thus imperative in examining ecological interactions. The provision of agricultural inputs in these resource‐scarce regions supports invasive and pest species, negatively impacting both agricultural productivity and native desert ecosystems. Understanding the spatial dynamics of invasive and pest species requires analyzing both bottom‐up resource availability factors underlying animal distributions and top‐down biological controls. Here, we evaluate the social‐ecological cascading effects of dryland agriculture on vertebrate pest communities in dryland agricultural communities of Israel. Our study region is characterized by 18 agricultural cooperatives with distinct crop regimes due to contrasting social decision‐making and resource allocation schemes (i.e., communal kibbutzim vs. privatized moshavim). Crop choices further affect land management (e.g., enclosed vs. open farm systems) and resource intensity. This system is ideal for studying trophic mechanisms underlying animal assemblages between agricultural regimes. We examine the role of agricultural land‐use practices on pest spatial distributions based on multiyear vertebrate pest observations with agricultural data sets. We use structural equation modeling (SEM) to quantify the relative importance of added agricultural resources underlying bottom‐up and top‐down trophic processes regulating vertebrate pest assemblages. Results reveal that crop choices determine pest distributions through bottom‐up processes directly, while simultaneously driving pest competitive interactions through indirect top‐down cascades impacting pest communities. For example, due to the indirect negative effect of wolves on mesopredators (foxes and jackals) mediated by livestock, the total positive effect of livestock on the abundance of mesopredators is reduced. Our study illustrates the social‐ecological cascading effects of agricultural regimes on pest community assemblages mediated by contrasting agricultural land‐use practices. Considering the expansion of dryland agroecological systems globally, understanding the intricate cascading pathways of predator‐ and prey‐pest communities has important implications for agricultural management, biological invasions in drylands, and fragile desert environments.
Examining changes in binge‐eating disorder network centrality and structure in patients treated with cognitive‐behavioral therapy versus interpersonal psychotherapy
Authors: Forrest Lauren N. , Franko Debra L. , Thompson‐Brenner Heather , Grilo Carlos M. 
Published: December 2022

AbstractObjectiveA sizeable minority of patients with binge‐eating disorder (BED) do not fully respond to evidence‐based treatments. Evidence to guide refinements of treatments is needed. Conceptualizing BED as arising from a network of symptom‐to‐symptom interactions allows for identification of the most strongly connected symptoms, which could inform intervention targets. This study estimated networks of BED features at pretreatment and posttreatment to assess whether cognitive behavioral therapy (CBT) and interpersonal psychotherapy (IPT) differentially impacted the interrelationships of BED symptoms/features.MethodsParticipants were 392 adults (83% women, 88% white) with BED who received CBT (n = 236) or IPT (n = 156) and assessed at pretreatment and posttreatment. Networks were estimated across timepoints and treatments. Expected influence (EI) was calculated; symptoms with the highest EI have the most strong and frequent associations with other symptoms. We also assessed whether the symptoms with the highest and lowest EI predicted posttreatment remission indicators.ResultsIn the CBT and IPT networks, shape concern, weight concern, and eating concern had the highest EI at pretreatment and posttreatment. EI significantly increased from pretreatment to posttreatment for some symptoms in CBT but did not change for any symptoms in IPT. Shape concern significantly and positively predicted BED remission indicators in CBT and IPT.ConclusionsCBT and IPT similarly impacted interrelations among BED features. Pretreatment EI predicted posttreatment remission indicators, indicating that pretreatment centrality could signal meaningful intervention targets. Clinical implications and avenues for future research are discussed including how personalized network analysis may advance the understanding of the clinical utility of centrality.Public SignificanceCognitive behavioral therapy and interpersonal therapy for binge‐eating disorder, which are two leading evidence‐based treatments for binge‐eating disorder that are quite different in their models and approaches, similarly impacted interrelations among binge‐eating disorder symptoms. In addition, the most strongly interconnected symptom predicted indicators of remission. Studying the interrelations among symptoms may provide new insight on how treatments impact symptom relationships and inform intervention targets.
Population genetic structure of the globally introduced big‐headed ant in Taiwan
Authors: Liu Kuan‐Ling , Tseng Shu‐Ping , Tatsuta Haruki , Tsuji Kazuki , Tay Jia‐Wei , Singham G. Veera , Yang Chin‐Cheng Scotty , Neoh Kok‐Boon 
Published: December 2022

AbstractGlobal commerce and transportation facilitate the spread of invasive species. The African big‐headed ant, Pheidole megacephala (Fabricius), has achieved worldwide distribution through globalization. Since the late 19th century, Taiwan has served as a major seaport because of its strategic location. The population genetic structure of P. megacephala in Taiwan is likely to be shaped by international trade and migration between neighboring islands. In this study, we investigated the population genetics of P. megacephala colonies sampled from four geographical regions in Taiwan and elucidated the population genetic structures of P. megacephala sampled from Taiwan, Okinawa, and Hawaii. We observed a low genetic diversity of P. megacephala across regions in Taiwan. Moreover, we noted low regional genetic differentiation and did not observe isolation by distance, implying that long‐distance jump dispersal might have played a crucial role in the spread of P. megacephala. We sequenced the partial cytochrome oxidase I gene and observed three mitochondrial haplotypes (TW1–TW3). TW1 and TW3 most likely originated from populations within the species' known invasive range, suggesting that secondary introduction is the predominant mode of introduction for this invasive ant. TW2 represents a novel haplotype that was previously unreported in other regions. P. megacephala populations from Taiwan, Okinawa, and Hawaii exhibited remarkable genetic similarity, which may reflect their relative geographic proximity and the historical connectedness of the Asia‐Pacific region.
Geography, taxonomy, and ecological guild: Factors impacting freshwater macroinvertebrate gut microbiomes
Authors: Ayayee Paul A. , Wesner Jeff S. , Ouellette Scot P. 
Published: December 2022

AbstractDespite their diversity, global distribution, and apparent effects on host biology, the rules of life that govern variation in microbiomes among host species remain unclear, particularly in freshwater organisms. In this study, we sought to assess whether geographic location, taxonomy (order, family, and genus), or functional feeding group (FFG) designations would best explain differences in the gut microbiome composition among macroinvertebrates sampled across 10 National Ecological Observatory Network's (NEON) freshwater stream sites in the United States. Subsequently, we compared the beta diversity of microbiomes among locations, taxonomy (order, family, and genus), and FFGs in a single statistical model to account for variation within the source microbial community and the types of macroinvertebrates sampled across locations. We determined significant differences in community composition among macroinvertebrate orders, families, genera, and FFGs. Differences in microbiome compositions were underscored by different bacterial ASVs that were differentially abundant among variables (four bacterial ASVs across the 10 NEON sites, 43 ASVs among the macroinvertebrate orders, and 18 bacterial ASVs differing among the five FFGs). Analyses of variations in microbiome composition using the Bray–Curtis distance matric revealed FFGs as the dominant source of variation (mean standard deviation of 0.8), followed by stream site (mean standard deviation of 0.5), and finally family and genus (mean standard deviation of 0.3 each). Our findings revealed a principal role for FFG classification in insect gut microbiome beta diversity with additional roles for geographic distribution and taxonomy.
Suppressed Internal Intrinsic Stress Engineering in High‐Performance Ni‐Rich Cathode Via Multilayered In Situ Coating Structure
Authors: Yang Jiachao , Li Yunjiao , Xi Xiaoming , Zheng Junchao , Yu Jian , He Zhenjiang 
Published: December 2022

LiNixCoyAlzO2(NCA) cathode materials are drawing widespread attention, but the huge gap between the ideal and present cyclic stability still hinders their further commercial application, especially for the Ni‐rich LiNixCoyAlzO2 (x > 0.8, x + y + z = 1) cathode material, which is owing to the structural degradation and particles' intrinsic fracture. To tackle the problems, Li0.5La2Al0.5O4 in situ coated and Mn compensating doped multilayer LiNi0.82Co0.14Al0.04O2 was prepared. XRD refinement indicates that La–Mn co‐modifying could realize appropriate Li/Ni disorder degree. Calculated results and in situ XRD patterns reveal that the LLAO coating layer could effectively restrain crack in secondary particles benefited from the suppressed internal strain. AFM further improves as NCA‐LM2 has superior mechanical property. The SEM, TEM, XPS tests indicate that the cycled cathode with LLAO–Mn modification displays a more complete morphology and less side reaction with electrolyte. DEMS was used to further investigate cathode–electrolyte interface which was reflected by gas evolution. NCA‐LM2 releases less CO2 than NCA‐P indexing on a more stable surface. The modified material presents outstanding capacity retention of 96.2% after 100 cycles in the voltage range of 3.0–4.4 V at 1C, 13% higher than that of the pristine and 80.8% at 1 C after 300 cycles. This excellent electrochemical performance could be attributed to the fact that the high chemically stable coating layer of Li0.5La2Al0.5O4 (LLAO) could enhance the interface and the Mn doping layer could suppress the influence of the lattice mismatch and distortion. We believe that it can be a useful strategy for the modification of Ni‐rich cathode material and other advanced functional material.
Potassium reduction with sodium zirconium cyclosilicate in patients with heart failure
Authors: Tardif Jean‐Claude , Rouleau Jean , Chertow Glenn M. , Al‐Shurbaji Ayman , Lisovskaja Vera , Gustavson Stephanie , Zhao Yanli , Bouabdallaoui Nadia , Desai Akshay S. , Chernyavskiy Alexander , Evsina Maria , Merkely Béla , McMurray John J.V. , Pfeffer Marc A. 
Published: December 2022

AbstractAimsSeveral patients with heart failure and reduced ejection fraction (HFrEF) do not receive renin–angiotensin–aldosterone system (RAAS) inhibitors at the recommended dose or at all, frequently due to actual or feared hyperkalaemia. Sodium zirconium cyclosilicate (SZC) is an orally administered non‐absorbed intestinal potassium binder proven to lower serum potassium concentrations.Methods and resultsPRIORITIZE‐HF was an international, multicentre, parallel‐group, randomized, double‐blind, placebo‐controlled study to evaluate the benefits and risks of using SZC to intensify RAAS inhibitor therapy. Patients with symptomatic HFrEF were eligible and randomly assigned to receive SZC 5 g or placebo once daily for 12 weeks. Doses of study medication and RAAS inhibitors were titrated during the treatment period. The primary endpoint was the proportion of patients at 12 weeks in the following categories: (i) any RAAS inhibitor at less than target dose, and no MRA; (ii) any RAAS inhibitor at target dose and no MRA; (ii) MRA at less than target dose; and (iv) MRA at target dose. Due to challenges in participant management related to the COVID‐19 pandemic, the study was prematurely terminated with 182 randomized patients. There was no statistically significant difference in the distribution of patients by RAAS inhibitor treatment categories at 3 months (P = 0.43). The proportion of patients at target MRA dose was numerically higher in the SZC group (56.4%) compared with the placebo group (47.0%). Overall, SZC was well tolerated.ConclusionsPRIORITIZE‐HF was terminated prematurely due to COVID‐19 and did not demonstrate a statistically significant increase in the intensity of RAAS inhibitor therapies with the potassium‐reducing agent SZC compared with placebo.
Total Synthesis of (E/Z) des‐Hydroxy Triticone A and B
Authors: Chakave Sunil Dattatray , Shete Shrikant , Kandukuri Sandeep Reddy , Montgomery Mark , Manjunath Bhanu N. , Sonawane Ravindra Punjaji 
Published: December 2022

AbstractThe first total synthesis of des‐hydroxy triticone A and B was achieved in 12 steps. The highly chemoselective, base mediated iodolactamization of olefins with N‐OMe amide motif is a key highlight of the synthesis.
A Simple, sensitive, and selective electrochemical aptasensor for cortisol based on rGO‐AuNPs
Authors: da Silva Alexsandra D. , Paschoalino Waldemir J. , Kubota Lauro T. 
Published: December 2022

AbstractCortisol is a steroid hormone naturally produced by the adrenal glands. It participates in and controls several processes in the body and is considered an important physiological biomarker. Due to its very low concentrations in body fluids, its detection requires high sensitivity and specificity. Here, we present a simple electrochemical biosensor based on reduced graphene oxide (rGO) modified with gold nanoparticles (AuNPs) for the immobilization of the cortisol‐specific aptamer (Ap) (MCH‐Ap‐AurGO/GCE). Important analytical parameters for identifying the target analyte were optimized, such as conditions and amount of immobilized Ap, the influence of the concentration and nature of the supporting electrolyte, pH of the medium, and incubation time. The optimized conditions for the aptasensor were: concentration of Ap 1.0 × 10−6 mol L−1, support electrolyte Tris/HCl 50 mmol L−1, MgCl2 10 mmol L−1, and NaCl 10 mmol L−1, at pH 5.0 and incubation time of 15 min. A linear response range was obtained from 1 × 10−18 up to 1 × 10−11 mol L−1 of cortisol with a detection limit (LOD) of 1.0 × 10−18 mol L−1. A curve adjusted for operational purposes in the saliva sample was fitted for the concentration range between 0.5 × 10−14 and 1 × 10−11 mol L−1, with a linear regression equation ΔRtc/Rtc1 = 2.70 + 0.17 × log([Cortisol]). The aptasensor demonstrated a great potential for detecting cortisol in a simple, fast, and highly sensitive way, opening its path for application in real samples, which present levels below the concentration in which cortisol is commonly found in body fluids.