bluuebunny/crossref_metadata_2025_split huggingface dataset:
The probability of developing posterior capsular opacification with Nd:YAG laser treatment after four types of IOL implantation
Authors: Ren Hong-Xing , Zhu Li , Wang Bin , Chen Bi-Feng , Sun Dai-Hong , Ye Ying-Jia 
Published: April 2023

Abstract
Background To compare the probability of posterior capsular opacification treatment with Nd:YAG laser after cataract extraction surgery with the AT Lisa tri 839 MP lens, ZMB00 lens, AA lens and ZCB00 lens, and to investigate the factors influencing posterior capsular opacification treatment with Nd:YAG laser.Methods We retrospectively analyzed patients who underwent cataract surgery with the AT Lisa tri 839 MP lens, ZMB00 lens, AA lens, and ZCB00 lens at our hospital from July 2018 to June 2021, analyzed the probability of posterior capsular opacification with Nd:YAG laser in these patients, as well as the factors affecting posterior capsular opacification with Nd:YAG laser treatment.Results A total of 395 eyes were treated with Nd:YAG laser in 317 patients, with an overall incidence of 6.1%. 14 eyes (42.4%) in the AT Lisa tri 839 MP group were treated with Nd:YAG laser, 3 eyes (1.9%) in the ZMB00 group were treated with Nd:YAG laser, 6 eyes (5.2%) in the AA group were treated with Nd:YAG laser, and 1 eye (1.1%) in the ZCB00 group was treated with Nd:YAG laser.Conclusion Hydrophilic IOLs with hydrophobic surface were more likely to have posterior capsular opacification treated with the Nd:YAG laser than hydrophobic IOLs .
The role of microRNA-155 in Viral Diseases
Authors: Biswas Susmita , Sarkar Neelakshi 
Published: April 2023

Abstract
On account of their potential to regulate gene expression, microRNAs are currently under the focus of modern antiviral research.miR-155 is a multifunctional microRNA that has a notable contribution to the regulation of the host immune system. Predictably, it also has a profound role in the pathogenesis of numerous viruses. More and more research has disclosed the involvement of miR-155 in various aspects of the host viral interface. Studies have also revealed that miR-155 or its inhibitor could be used as a potential tool in viral therapeutics and diagnosis. The current review attempts to highlight viral diseases which have been shown to be considerably modulated by miR-155. This is particularly important as exogenous molecular control of miR-155 expression could open new avenues to confine viral proliferation.
Imbalanced motivated behaviors according to motor sign asymmetry in drug-naïve Parkinson’s Disease
Authors: Béreau Matthieu , Castrioto Anna , Servant Mathieu , Lhommée Eugénie , Desmarets Maxime , Bichon Amélie , Pelissier Pierre , Schmitt Emmanuelle , Klinger Hélène , Longato Nadine , Phillipps Clélie , Wirth Thomas , Fraix Valérie , Benatru Isabelle , Durif Franck , Azulay Jean-Philippe , Moro Elena , Broussolle Emmanuel , Thobois Stéphane , Tranchant Christine , Krack Paul , Anheim Mathieu 
Published: April 2023

Abstract
Few studies have considered the influence of motor sign asymmetry on motivated behaviors in de novo drug-naïve Parkinson’s disease (PD). We tested whether motor sign asymmetry could be associated with different motivated behaviors patterns in de novo drug naïve PD. We performed a cross-sectional study in 149 de novo drug-naïve Parkinson’s disease patients and used the Ardouin Scale of Behavior in Parkinson’s disease (ASBPD) to assess a set of motivated behaviors. We assessed motor asymmetry based on i) the side of motor onset and ii) the MDS-UPDRS motor score, then we compared right hemibody Parkinson’s disease to left hemibody Parkinson’s disease. Patients with de novo right hemibody onset PD had significantly lower scores on the ASBPD for nocturnal hyperactivity (p = 0.031), eating behavior (p = 0.008), and approach behavior composite scores (p = 0.004) than patients with de novo left hemibody onset PD. Patients with de novo left hemibody onset PD had significantly higher scores on the ASBPD for anxiety (p = 0.025) than patients with de novo right hemibody onset PD. Our findings suggest that motor sign asymmetry may be associated with an imbalance between motivated behaviors in de novo drug-naïve Parkinson’s disease.
von Willebrand factor-binding protein (vWbp)-activated Factor XIII and transglutaminase 2 (TG2) promote cross-linking between FnBPA from Staphylococcus aureus and fibrinogen
Authors: Motta Chiara , Pellegrini Angelica , Camaione Stefano , Geoghegan Joan A , Speziale Pietro , Barbieri Giulia , PIETROCOLA GIAMPIERO 
Published: April 2023

Abstract
The secreted von Willebrand factor-binding protein (vWbp) from Staphylococcus aureusinteracts with the coagulation factors prothrombin and fibrinogen (Fbg), leading to the non-proteolytic transglutaminase activation of Factor XIII (FXIII). In this study we found that vWbp-activated FXIII catalyses the incorporation of amino-donor dansylcadaverine into region A of fibronectin-binding protein A (FnBPA). Incubation of Fbg with recombinant region A of S. aureus Fbg-binding proteins FnBPA, FnBPB, ClfA or ClfB in presence of vWbp-activated FXIII resulted in the formation of high molecular heteropolymers with FnBPA only, suggesting a specificity of the cross-linking reaction between fibrin(ogen) and the staphylococcal surface. As previously observed, cross-linking sites were mapped to the a-chain and the N1 subdomain of fibrin(ogen) and region A of FnBPA, respectively. Comparable results were obtained when tissue tranglutaminase-2 (TG2) was tested for cross-linking of FnBPA and Fbg. Of note, FnBPA-mediated covalent cross-linking promoted by vWbp-activated FXIII was also observed when bacteria were allowed to attach to fibrin(ogen). Together these findings suggest a novel pathogenetic mechanism by which the transglutaminase action of FXIII and/or TG2 contributes to entrapment and persistence of S. aureus in blood and host tissues.
“Grade-1 Skin-only Acute-gvhd”: One Week After Rash Appearance as the Time Point to Decide on Treatment
Authors: Milone Giuseppe , Cupri Alessandra , Leotta Salvatore , Giuffrida Giulia , Milone Giulio , Sapuppo Gabriele , Giunta Giuliana , Esposito Benedetta , Leotta Dario , Fiore Sebastiano , Pennisi Chiara , Longo Ermelinda 
Published: April 2023

Abstract
After allogeneic hematopoietic transplantation, a limited skin a-GVHD (Grade 1 a-GVHD) is usually managed by topical treatment and the criteria to start systemic immunosuppressive therapy are not established. We performed a prospective observational study on 102 consecutive adult patients who underwent allogeneic HSC transplantation. Eighty-two presented a skin rash (80.3%) and thirty-one of them (37.8%) progressed to Grade 2- some within 48 hours (Group 1) while others within days 3–7 (Group 2). Fifty-one patients (62.2%) did not have progression to Grade 2 GVHD within 7 days (Group 3). These patients were followed up. The Group 3 patients were subdivided into Group 3A (these had a stable or fading rash from the day of appearance to day 8) and Group 3B (these had a worsening rash from appearance to day 8). In group 3A the clinical results were significantly superior compared to results obtained in group 3B patients. In Group 3A versus Group 3B, Overall Survival at 2 years was 75.1% vs 39.8% (p = 0.03), Treatment Related mortality was 10.4% vs 39.2% (p = 0.05), need for secondary anti-GVHD treatment was 46.6% vs 76.4% (p = 0.002). The dynamic of skin rash during the first week from appearance marks patients with different prognosis.
Gender Differences in Non-Alcoholic Fatty Liver Disease in Obese Children and Adolescents: A Large Cross-sectional Study
Authors: Jin Binghan , Wu Zhaoyuan , Wang Shan , Yu Zhu , Ullah Rahim , Liang Xinyi , Wu Wei , Huang Ke , Ni Yan , Wang Jianbing , Dong Guanping , Fu Junfen 
Published: April 2023

Abstract
Objective: Previous studies have reported sex differences in non-alcoholic fatty liver disease (NAFLD) in adults; however, little is known in children and adolescents. This study aims to examine their NAFLD prevalence and investigate the relationship between sex hormones and NAFLD.
Method: 2999 Chinese obese patients aged 2-18 years were included in the study. Liver ultrasound, anthropometry, blood test, and oral glucose tolerance test were performed. We examined the prevalence of NAFLD by sex, age, and Tanner stages. Regression model and principal component factor analysis were used.
Results: The prevalence of NAFLD increased with age in both sexes and the gender difference appeared before puberty. The prevalence in boys tended to be stable at the age of 11 years, whereas the girls reached the peak temporarily. NAFLD prevalence was positively associated with estradiol in boys (p = 0.011), but the opposite trend was observed in girls (P = 0.031). Testosterone levels decreased with the increase of NAFLD prevalence (P < 0.001). Luteinizing hormone and prolactin were inversely associated with NAFLD prevalence in boys and girls, respectively. Results from the principal component analysis showed that sex hormones levels and abdominal obesity were important risk factors for the prevalence of NAFLD in obese children (P<0.001).
Conclusion: The notable difference in NAFLD prevalence between genders in obese children starts in early childhood. The difference arises long before puberty onset and tends to stabilize during late adolescence. Sex hormones are associated with NAFLD prevalence and are influenced by the Tanner stages and fat distribution.
Macroeconomic Effects of Energy Efficiency Measures and Carbon Tax in Thailand: A Computable General Equilibrium Analysis
Authors: Pradhan Bijay B. , Rajbhandari Salony , Chaichaloempreecha Achiraya , Limmeechokchai Bundit 
Published: April 2023

Abstract
Energy efficiency improvement is one of the measures undertaken by government of Thailand to achieve its Nationally Determined Contribution (NDC) by 2030 and contribute towards carbon neutrality 2050. The objective of this study is to compare the economy of Thailand before and after the implementation of energy efficiency improvement. This study also assessed the rebound effects of energy efficiency improvement and further analyzed the role of carbon tax in minimizing the rebound effects. In addition, the effects of GHG emissions with energy efficiency improvement and carbon tax were also assessed. The study was analyzed by developing a static computable general equilibrium (CGE) model of Thailand. The CGE model consists of 27 non-energy sectors and 5 energy sectors. This study finds that the energy efficiency improvement in the production sectors would lower the energy consumption in the overall production sectors but increase the consumption in the household sector and its associated GHG emissions from the energy use. However, energy efficiency improvement in both households and production sectors would cause a decline in the overall energy consumption and GHG emissions. Results show the rebound effects in the aforementioned energy efficiency improvement cases. It is found that the implementation of carbon tax of $5/tCO2e would not be enough to neutralize the rebound effects, however, carbon tax of $10/tCO2e would be sufficient to neutralize the rebound effects with GDP gain.
Mental health status and Stressful life events among postgraduate students in Cyprus: A cross-sectional descriptive correlational study
Authors: Sokratis Sokratous , Giorgos Alexandrou , Rafailia Zavrou , Maria Karanikola 
Published: April 2023

Abstract
Background Despite prior evidence supporting the association between stressful life events and mental health status, there are limited data on the frequency and severity of stressful life events and their effects on university students’ mental health status. Therefore, the present study aimed to: a) examine mental health status and subsequent predictors of clinically significant mental distress symptoms, b) estimate the frequency and number of stressful life events, and c) explore the associations between the above and sociodemographic variables.Methods This study was descriptive, cross-sectional, and correlational with internal comparisons. A convenience sample of 92 Master of Science students participated in the study. Participants with a General Health Questionnaire-28 (GHQ-28) total score ≥ 24 were considered to have clinically significant mental distress symptoms. Descriptive and inferential statistics were evaluated, and Pearson's chi-square test for group differences was used to analyse the data. Analysis of variance and t-tests were used for comparisons between two or more groups, and regression analyses were employed to identify the predictors of GHQ-28 scores and clinical mental distress symptoms.Results The final sample comprised 90 students (response rate: 97.8%), 33 (36.7%) of whom reported clinically significant symptoms of mental distress. Students with divorced parents [29.92 (± 10.62), p < .05] scored high on the GHQ-28. Participants who had low/no satisfaction with the education system posted higher scores than participants who had high/very high satisfaction [24.82 (± 11.68) vs 17.93 (± 9.78), p < .05]. In the subscale measuring depressive symptoms, there was a statistically significant gender difference, with men reporting higher scores than females. [3.0± (3.69) vs 1.60 (± 2.42), p = .034]. In multiple analyses of sociodemographic characteristics and those who scored higher on the Life Events Scale for Students (LESS) scale (≥ 340), the loss of parent/s was associated with the total GHQ-28 score (B=-17.046, p < .001). In multiple analyses, chronic physical disorders or disabilities and numerous stressful life events among students (≥ 8 events) were correlated with higher overall GHQ-28 scores (B = 15.232, p < .005).Conclusion The high frequency of clinical symptoms of mental distress among postgraduate university students and their correlation with stressful events highlights the need for university counselling services to provide psychological support strategies to students.
Gut microbiota modulatory capacity of Brassica oleracea italica x alboglabra (Bimi ® )
Authors: Rosés Carles , Viadel Blanca , Nieto Juan Antonio , Soriano-Romaní Laura , Romo-Hualde Ana , Agudelo Agatha , Milagro Fermín I. , Barceló Anna 
Published: April 2023

Abstract
Many human diseases, including obesity and its comorbidities, have been related with changes in the gut microbiota profile, specifically with changes in bacterial diversity and richness. The main goal of the present work is to investigate the modulatory effect of Brassica oleracea italica x alboglabra (Bimi®) on gut microbiota composition through an in-vitro digester and an in-vivo trial. The digester simulates the entire gastrointestinal digestive process by using five interconnected compartments (stomach, small intestine and the three colonic sections). The human trial included 15 Spanish adults who included 90 g of Bimi® every two days into their habitual diet. This clinical trial has been registered at ClinicalTrials.gov with the reference NCT04799223 and date of registration of March 16th, 2021. In vitro results showed many short chain fatty acids (SCFA)-producing bacteria favoured by synergetic effects of the fibres, resistant starch and polyphenols present in Bimi®. The human intervention confirmed some of these results and evidenced an increase in beneficial bacteria, suggesting that Bimi® is capable of modulating gut microbiota towards a healthier status, with great potential in the management of human disorders related to microbiota dysbiosis.
Association between prior-night sleep and next-day fatigue in older adults: a daily diary study
Authors: Nakagawa Takeshi , Yasumoto Saori , Kabayama Mai , Matsuda Ken’ichi , Gondo Yasuyuki , Kamide Kei , Ikebe Kazunori 
Published: April 2023

Abstract
Background: Fatigue is known as an element of frailty. Short sleep duration increases fatigue in older adults. However, the relationship between long sleep and fatigue remains unclear. Using a daily diary method, this study examined the within- and between-person associations between sleep and fatigue in older adults.
Methods:The study recruited 56 Japanese community dwellers (age: 82–86 years; female: 37.5%). Participants responded to a daily diary questionnaire at the end of each day. Over seven days, time in bed and satisfaction were measured after waking up, whereas fatigue was assessed before going to bed. We included person-level covariates (demographic factors, and physical and mental health) and day-level covariates (time in study, and positive and negative emotions). Multilevel models were estimated to examine within- and between-person associations.
Results: At the within-person level, on days following short and long time in bed and days following low levels of sleep satisfaction, individuals felt higher levels of fatigue compared with usual days. At the between-person level, no statistically significant differences in fatigue were observed between individuals with long and short time in bed.
Conclusions:The findings suggest that prior-day sleep is associated with next-day fatigue in older adults. Long and short sleep duration and low sleep quality can lead to fatigue. Enhanced management of sleep behavior may reduce fatigue.
Belowground plant traits and hydrology control microbiome composition and methane flux in temperate fen mesocosms
Authors: Piecha Marc , Kreyling Jürgen , Couvenberg John , Pester Michael , Günther Anke , Jurasinski Gerald , Blume-Werry Gesche , Urich Tim , Wang Haitao 
Published: April 2023

Abstract
The rewetting of formerly drained peatlands is a strategy to fight against global warming through the reduction of CO2 emissions, although this can lead to elevated CH4 emissions. The interplay between plants, hydrology and microbiomes as ultimate determinants of CH4 dynamics is still poorly understood, despite recent progress in field studies. Using a mesocosm approach, we simulated the re-cultivation of a degraded temperate fen with three different water levels and two different plant over the course of a growing season. Peat samples for microbiome analysis, above- and below-ground plant biomass and gas fluxes were measured in April, June, August and October. Microbiome composition in top and subsoils was determined using 16S rRNA gene amplicon sequencing. We found that peat depth and sampling time were the major factors shaping the microbiome composition dynamics. While plant species had a less strong impact, the difference to bare ground microbiomes was significant, especially in the lower layer. The water status also affected the microbiome, albeit to a much lesser extent. Methanogens were most abundant in the deeper peat and also more abundant in bare ground and Carex rostrata pots, as compared to Juncus inflexus or mixed pots. This was inversely linked to the larger root network size of J. inflexus. The methane emissions correlated positively with the abundance of methanogens and correlated negatively with the root network size. In conclusion, this interdisciplinary study sheds light on how the complex interplay between plants, hydrology and the fen microbiome affect CH4 emissions. It showed that the presence of plants as well as the plant functional type determine the abundance of methanogens and microbiome composition and thereby the resulting CH4 fluxes accordingly.
Clonal dynamics after allogeneic haematopoietic cell transplantation using genome-wide somatic mutations
Authors: Campbell Peter , Chapman Michael Spencer , Wilk Matthias , Boettcher Steffen , Mitchell Emily , Dawson Kevin , Williams Nicholas , Muller Jan , Kovtonyuk Larisa , Jung Hyunchul , Caiado Francisco , Roberts Kirsty , O'Neill Laura , Kent David , Green Anthony , Nangalia Jyoti , Manz Markus 
Published: April 2023

Abstract

Allogeneic haematopoietic cell transplantation (HCT) replaces the stem cells responsible for blood production with those harvested from a donor, and is received by 40,000 patients worldwide each year. To quantify dynamics of long-term stem cell engraftment, we sequenced whole genomes of 2,824 single-cell-derived haematopoietic colonies from blood samples of 10 donor-recipient pairs taken 9-31 years after HLA-matched sibling HCT. With younger donors, 10,000–50,000 stem cells had engrafted and were still contributing to haematopoiesis at time of sampling, but estimates were 10-fold lower with older donors. Engrafted stem cells made multilineage contributions to myeloid, B-lymphoid and T-lymphoid populations, although individual clones often showed biases towards one or other mature cell type. Recipients had lower clonal diversity than matched donors, equivalent to ~10-15 years of additional ageing, arising from up to 25-fold greater expansion of stem cell clones. An HCT-related population bottleneck alone could not explain these differences: instead, phylogenetic trees evinced two distinct modes of HCT-specific selection. In ‘pruning selection’, cell divisions underpinning recipient-enriched clonal expansions had occurred in the donor, preceding transplant – their selective advantage derived from preferential mobilisation, harvest, survival ex vivo or initial homing. In ‘growth selection’, cell divisions underpinning clonal expansion occurred through proliferative advantage in the recipient’s marrow after homing – clones with multiple driver mutations especially demonstrated this pattern. Uprooting stem cells from their native environment and transplanting them to foreign soil exaggerates selective pressures, distorting and accelerating the loss of clonal diversity compared to the unperturbed haematopoiesis of donors.
Intercropping of Soybean within Maize for increasing the crop productivity and cropping intensity in Coastal Region of Bangladesh
Authors: Hasan Gazi Nazmul , Khan Md Mahmudul Hasan , Anik Md Rashidul Hasan , Mahmud Md Hasan 
Published: April 2023

Abstract
A field trial was conducted at Bhola sadar and Daulatkhan during the rabi season of 2020-21 and 2021-22 at the farmer’s field under AEZ-18 to verify the agro-economic performance of intercropping of Soybean with Maize and to ensure the maximum utilization of the land for higher yield, Land Equivalent Ratio (LER) and economic return. The experiment was laid out in Randomized Complete Block Design on farmer’s field condition in Bhola sadar. Four treatments combination of different seed rate of mustard and cowpea viz., T1= Sole maize, T2= Sole Soybean, T3= Two row maize (60cm x 25cm) with 2 row Soybean (30cm x 6cm), T4= Pair row maize (120cm x 25cm) with 4 row Soybean (30cm x 6cm). Variety was BARI Hybrid Maize-16 and BARI Soybean-6. In case of maize no. of cobs plant-1, 1000-grain wt. (g) of maize was significantly influenced by intercrop combination in two consecutive years. Plant population was higher (7.13cm & 6.87 cm) in T1 treatments followed by T3 treatments (7.13cm & 6.87cm) and lower plant population was from T4 treatments (6.38cm & 6.12cm) due to pair row maize combination in two consecutive years. The highest no. of grains cob-1 (545 & 558) was recorded from T1 followed by T4 (536 & 539) and the lowest (518 & 496) was obtained from T3 treatment in 2021 & 2022 respectively. The highest maize grain yield (7.83 & 7.92 t ha-1) was obtained from sole maize in both the years. Grain yield (1.72 & 1.82 t ha-1) of sole soybean was significantly higher and lowest soybean grain yield (1.30 & 1.25 t ha-1) was obtained from T3 treatment in 2021 & 2022 respectively. The highest Maize Equivalent Yield 9.41 t ha-1 and 9.63 t ha-1 was recorded from T3 intercropped combination in two consecutive years (Table 3a & 3b). In T4 treatment, the LER was higher 1.80 & 1.71 and LER was lower 1.67 & 1.60 from T3 treatment in both years. Growing maize and soybean in intercropping under rainfed condition resulted in higher LER (Table 4) when grown as T3 and T4 indicting beneficial association between the two.