bluuebunny/crossref_metadata_2025_split huggingface dataset:
Substantially Delayed Maturation of Growth Plate Chondrocytes in “Humanized” PTH1R Mice with the H223R Mutation of Jansen's Disease
Authors: Reyes Monica , Firat Damla , Hanna Patrick , Khan Mohd , Bruce Michael , Shvedova Maria , Kobayashi Tatsuya , Schipani Ernestina , Gardella Thomas J. , Jüppner Harald 
Published: August 2023

AbstractActivating parathyroid hormone (PTH)/PTH‐related Peptide (PTHrP) receptor (PTH1R) mutations causes Jansen's metaphyseal chondrodysplasia (JMC), a rare disease characterized by growth plate abnormalities, short stature, and PTH‐independent hypercalcemia. Previously generated transgenic JMC mouse models, in which the human PTH1R allele with the H223R mutation (H223R‐PTH1R) is expressed in osteoblasts via type Ia1 collagen or DMP1 promoters cause excess bone mass, while expression of the mutant allele via the type IIa1 collagen promoter results in only minor growth plate changes. Thus, neither transgenic JMC model adequately recapitulates the human disease. We therefore generated “humanized” JMC mice in which the H223R‐PTH1R allele was expressed via the endogenous mouse Pth1r promoter and, thus, in all relevant target tissues. Founders with the H223R allele typically died within 2 months without reproducing; several mosaic male founders, however, lived longer and produced F1 H223R‐PTH1R offspring, which were small and exhibited marked growth plate abnormalities. Serum calcium and phosphate levels of the mutant mice were not different from wild‐type littermates, but serum PTH and P1NP were reduced significantly, while CTX‐1 and CTX‐2 were slightly increased. Histological and RNAscope analyses of the mutant tibial growth plates revealed markedly expanded zones of type II collagen‐positive, proliferating/prehypertrophic chondrocytes, abundant apoptotic cells in the growth plate center and a progressive reduction of type X collagen‐positive hypertrophic chondrocytes and primary spongiosa. The “humanized” H223R‐PTH1R mice are likely to provide a more suitable model for defining the JMC phenotype and for assessing potential treatment options for this debilitating disease of skeletal development and mineral ion homeostasis. © 2023 The Authors. JBMR Plus published by Wiley Periodicals LLC on behalf of American Society for Bone and Mineral Research.
A 219‐year reconstruction of April–June mean minimum temperature from the tree‐ring earlywood density on the Changbai Mountains, China
Authors: Abudureheman Ruxianguli· , Zhang Tongwen , Wang Yonghui , Yu Shulong , Zhang Ruibo , Yuan Yujiang , Guo Dong 
Published: August 2023

AbstractUsing mean earlywood density data from Pinus koraiensis cores, we reconstructed the April–June mean minimum temperature from 1797 to 2015 for the Changbai Mountains. The reconstruction explained 52.6% of the actual mean minimum temperature variance during 1901–2015. After smoothing with an 11‐year moving average, we identified three major cold periods (1797–1875, 1881–1899, and 1903–1946) and three major warm periods (1876–1880, 1900–1902, and 1947–2015) in the reconstructed series. Spatial correlation showed that the reconstruction contained climatic signals for a large area including most of northeast China, especially the Bohai Rim region and the eastern side of the Mongolian Plateau. A comparison between the newly reconstructed temperature series for the surrounding areas reveals similar variations, particularly in the warming trend during the second half of the 20th century. The driving factors of the mean minimum temperature were influenced mainly by the interaction of solar activity and large‐scale atmospheric‐oceanic variability. The reconstructed series provides a basis for understanding past climate change in the Changbai Mountains and for predicting future climate change trends in Northeast China.
Omics shed light on the mechanisms of petroleum‐contaminated soil remediation: When biochar meets nitrogen
Authors: Kong Dexu , Liu Zhe , Wu Juan 
Published: August 2023

AbstractDecontamination of oil‐affected soil is an urgent worldwide issue. In this study, a microcosm experiment was conducted to explore the effect of combined utilization of urea and biochar on oil‐contamination remediation in an eastern China oil‐field soil and explored their impact on soil physicochemical properties, organic pollutants degrading‐related enzyme and functional genes, microbiome, and metabolome. Then, a pot experiment was conducted to verify the impact of the remediation experiment. Results showed that a combined application of biochar and nitrogen reduced 78.6% of total petroleum hydrocarbons in soil. Combined treatment enhanced soil dehydrogenase and catalase activities, alkB, and CYP gene abundances relative to Control. Moreover, nitrogen input reduced soil microbial α‐diversity, while improving the relative abundances of Alcanivorax and Pseudogym. In addition, the pathways of glyoxylate and dicarboxylate metabolism, naphthalene and anthracene degradation, butanoate metabolism, pyruvate metabolism, and glycolysis or gluconeogenesis were up‐regulated in combined treatment relative to Control. Besides, both biochar and nitrogen addition can increase the number of edges and clustering coefficient of the microbial network, and improve the network robustness. The pot experiment showed that combined treatments enhanced ryegrass shoot length, root length, and biomass by 57.8%, 38.5%, and 42.8%, respectively. Overall, this study proved the feasibility of combined biochar and urea co‐application in remedying oil‐contaminated soil and shed light on the inner mechanism.
Drought monitoring performance of global precipitation products in three wet seasons in Ethiopia: Part I—Quasi‐objective examination
Authors: Degefu Mekonnen Adnew , Bewket Woldeamlak 
Published: August 2023

AbstractThe need for representative and accurate climate data such as precipitation useful for drought monitoring has been rapidly increasing among policymakers and practitioners to tackle climate‐change‐induced drought events. Hence, the objective of this article is to evaluate the drought monitoring performance of global precipitation products for the three wet seasons and rainfall regions in Ethiopia. Drought indices were calculated using the Standardized Precipitation Index (SPI) at 3‐month timescale for Belg (March–May) and Autumn (September–November) seasons and at 4‐month timescale for the Kiremt (June–September) seasons. Data products were evaluated for their accuracy in representing drought magnitude, geographical coverage and frequency using quasi‐objective (visual inspection), and frequency and correlation analysis methods. The performance of gridded precipitation products was compared against the SPI value computed for 126 reference stations and the Ethiopian satellite‐gauge merged precipitation data. Precipitation products showed different levels of performance in representing the magnitude, frequency and geographical coverage of drought events for the three wet seasons and rainfall regions. None of the data products outperformed in representing the occurrence of drought for all three wet seasons and the corresponding three rainfall regions. However, the Ethiopian merged precipitation, Famine Early Warning Systems Network (FEWS NET) Land Data Assimilation System (FLDAS) and Climate Hazard Group InfraRed Precipitation with Stations (CHIRPS) precipitation products are relatively better than others. The study results generally indicate that no single data outperform the other precipitation products in representing the complex spatiotemporal characteristics of drought events in a mountainous region like Ethiopia.
Borel resummation of secular divergences in stochastic inflation
Authors: Honda Masazumi , Jinno Ryusuke , Pinol Lucas , Tokeshi Koki 
Published: August 2023

Abstract
We make use of Borel resummation to extract the exact time dependence from the divergent series found in the context of stochastic inflation. Correlation functions of self-interacting scalar fields in de Sitter spacetime are known to develop secular IR divergences via loops, and the first terms of the divergent series have been consistently computed both with standard techniques for curved spacetime quantum field theory and within the framework of stochastic inflation. We show that Borel resummation can be used to interpret the divergent series and to correctly infer the time evolution of the correlation functions. In practice, we adopt a method called Borel-Padé resummation where we approximate the Borel transformation by a Padé approximant. We also discuss the singularity structures of Borel transformations and mention possible applications to cosmology.
Anomalous triple gauge couplings in electroweak dilepton tails at the LHC and interference resurrection
Authors: Hwang Haeyun , Min Ui , Park Junghyeon , Son Minho , Yoo Jae Hyeok 
Published: August 2023

Abstract
We study the electroweak dilepton production with two forward jets at the LHC, aiming to measure the anomalous triple gauge couplings in the Effective Field Theory (EFT) approach. This process exhibits a distinctive feature, namely, the interference between Standard Model (SM) and beyond the SM is resurrected in the inclusive cross section of the full amplitude, including two forward jets. As a concrete illustration, we perform the detailed analytic and numerical study of the interference using a simpler toy process, and discuss the subtlety of the effective W approximation. We propose a new kinematic variable, VBFhardness, that controls the amount of energy flowing into the dilepton subprocess. We show that an appropriate cut on VBFhardness makes the interference resurrection manifest. Finally, we use the invariant mass of the dilepton system as well as the transverse momentum, as done in the literature, to derive the sensitivity to anomalous triple gauge couplings at the LHC and the high luminosity LHC. Our result is compared with the existing limits from the experiments.
Bridging the static patches: de Sitter holography and entanglement
Authors: Franken Victor , Partouche Hervé , Rondeau François , Toumbas Nicolaos 
Published: August 2023

Abstract
In the context of de Sitter static-patch holography, two prescriptions have been put forward for holographic entanglement entropy computations, the monolayer and bilayer proposals. In this paper, we reformulate both prescriptions in a covariant way and extend them to include quantum corrections. We argue that the bilayer proposal is self-consistent, while the monolayer proposal exhibits contradictory behavior. In fact, the bilayer proposal leads to a stronger holographic description, in which the full spacetime is encoded on two screens at the cosmological horizons. At the classical level, we find large degeneracies of minimal extremal homologous surfaces, localized at the horizons, which can be lifted by quantum corrections. The entanglement wedges of subregions of the screens exhibit non-trivial behaviors, hinting at the existence of interesting phase transitions and non-locality in the holographic theory. In particular, while each screen encodes its corresponding static patch, we show that the entanglement wedge of the screen with the larger quantum area extends and covers the causal diamond between the screens, with a phase transition occurring when the quantum areas of the screens become equal. We argue that the capacity of the screens to encode the region between them is lost, when these are pushed further in the static patches of the observers and placed on stretched horizons.
Atorvastatin lowers 68Ga-DOTATATE uptake in coronary arteries, bone marrow and spleen in individuals with type 2 diabetes
Authors: Oostveen Reindert F. , Kaiser Yannick , Ståhle Mia R. , Nurmohamed Nick S. , Tzolos Evangelos , Dweck Marc R. , Kroon Jeffrey , Murphy Andrew J. , Dey Damini , Slomka Piotr J. , Verberne Hein J. , Stroes Erik S. G. , Hanssen Nordin M. J. 
Published: August 2023

Abstract
Aims/hypothesis
Inflammation is a core component of residual cardiovascular risk in type 2 diabetes. With new anti-inflammatory therapeutics entering the field, accurate markers to evaluate their effectiveness in reducing cardiovascular disease are paramount. Gallium-68-labelled DOTATATE (68Ga-DOTATATE) has recently been proposed as a more specific marker of arterial wall inflammation than 18F-fluorodeoxyglucose (18F-FDG). This study set out to investigate whether 68Ga-DOTATATE uptake is amenable to therapeutic intervention in individuals with type 2 diabetes.

Methods
Individuals aged >50 years with type 2 diabetes underwent 68Ga-DOTATATE positron emission tomography (PET)/computed tomography (CT) at baseline and after 3 months treatment with atorvastatin 40 mg once daily. Primary outcome was the difference in coronary 68Ga-DOTATATE uptake, expressed as target-to-background ratio (TBR). The secondary outcome was difference in bone marrow and splenic uptake, expressed as the standardised uptake value (SUV).

Results
Twenty-two individuals with type 2 diabetes (mean age 63.2±6.4 years, 82% male, LDL-cholesterol 3.42±0.81 mmol/l, HbA1c 55±12 mmol/mol [7.2%±3.2%]) completed both 68Ga-DOTATATE PET/CT scans. The maximum TBR was −31% (95% CI −50, −12) lower in the coronary arteries, and bone marrow and splenic 68Ga-DOTATATE uptake was also significantly lower post statin treatment, with a mean percentage reduction of −15% (95% CI −27, −4) and −17% (95% CI −32, −2), respectively.

Conclusions/interpretation
68Ga-DOTATATE uptake across the cardio–haematopoietic axis was lower after statin therapy in individuals with type 2 diabetes. Therefore, 68Ga-DOTATATE is promising as a metric for vascular and haematopoietic inflammation in intervention studies using anti-inflammatory therapeutics in individuals with type 2 diabetes.

Trial registration
ClinicalTrials.gov NCT05730634

Graphical Abstract
IFNɣ but not IFNα increases recognition of insulin defective ribosomal product-derived antigen to amplify islet autoimmunity
Authors: Thomaidou Sofia , Munoz Garcia Amadeo , de Lange Sabine , Gan Jin , van der Slik Arno R. , Hoeben Rob C. , Roep Bart O. , Carlotti Françoise , Zaldumbide Arnaud 
Published: August 2023

Abstract
Aims/hypothesis
The inflammatory milieu characteristic of insulitis affects translation fidelity and generates defective ribosomal products (DRiPs) that participate in autoimmune beta cell destruction in type 1 diabetes. Here, we studied the role of early innate cytokines (IFNα) and late immune adaptive events (IFNɣ) in insulin DRiP-derived peptide presentation to diabetogenic CD8+ T cells.

Methods
Single-cell transcriptomics of human pancreatic islets was used to study the composition of the (immuno)proteasome. Specific inhibition of the immunoproteasome catalytic subunits was achieved using siRNA, and antigenic peptide presentation at the cell surface of the human beta cell line EndoC-βH1 was monitored using peptide-specific CD8 T cells.

Results
We found that IFNγ induces the expression of the PSMB10 transcript encoding the β2i catalytic subunit of the immunoproteasome in endocrine beta cells, revealing a critical role in insulin DRiP-derived peptide presentation to T cells. Moreover, we showed that PSMB10 is upregulated in a beta cell subset that is preferentially destroyed in the pancreases of individuals with type 1 diabetes.

Conclusions/interpretation
Our data highlight the role of the degradation machinery in beta cell immunogenicity and emphasise the need for evaluation of targeted immunoproteasome inhibitors to limit beta cell destruction in type 1 diabetes.

Data availability
The single-cell RNA-seq dataset is available from the Gene Expression Omnibus (GEO) using the accession number GSE218316 (https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE218316).

Graphical Abstract
Evaluation of sexual function and vaginal prolapse after radical cystectomy in women: a study to explore an under-evaluated problem
Authors: Wenk Maren Juliane , Westhoff N. , Liedl B. , Michel M. S. , Grüne B. , Kriegmair M. C. 
Published: August 2023

Abstract
Introduction and hypothesis
The objective was to evaluate sexual function, vaginal prolapse, and quality of life (QoL) in women after radical cystectomy (RC) using validated questionnaires and pelvic organ prolapse quantification (POP-Q) measurement.

Methods
Female bladder cancer patients who underwent RC at our tertiary care center were included (January 2008 to March 2022). Patients received three validated questionnaires (International Consultation on Incontinence Questionnaire Vaginal Symptoms [ICIQ-VS] Part A, Pelvic Organ Prolapse/Urinary Incontinence Sexual Questionnaire IUGA revised [PISQ], European Organization for Research and Treatment of Cancer Quality of Life Questionnaire [EORTC] C30/BLM30). Patients who consented were examined with vaginal POP-Q measurement.

Results
Out of 322 patients, 193 were still alive, 54 patients were lost to follow-up, and 43 were excluded, resulting in 96 patients who received the questionnaire. Finally, 35 patients were included, of whom 17 patients consented to vaginal examination. Complaints due to vaginal symptoms were low (ICIQ-VS 6.17 + 5.37). Sexual activity was reported by 12 patients (34.3%); 23 patients (65.71%) were not sexually active. No apical prolapse was found in POP-Q measurement; 6 patients (35.3%) had anterior, and 14 patients (82.4%) posterior prolapse; the highest prolapse stage was 2. No significant differences were found regarding POP stages, sexual function, and QoL (all p > 0.05) when comparing continent and incontinent urinary diversions. Comparing the vaginal approach (no sparing vs sparing), significant differences were found in only two PISQ subscales (significantly higher scores after vagina sparing, p = 0.01 and p = 0.02).

Conclusions
The type of urinary diversion, POP-Q stages, and tumor stages did not show significant differences regarding sexual function, QoL, and prolapse complaints in women after RC, whereas a vagina- sparing approach showed significant differences only in two subscales without clinical relevance.
Reproductive experience alters the effects of diazepam and fluoxetine on anxiety-like behaviour, fear extinction, and corticosterone levels in female rats
Authors: Pestana Jodie E. , Graham Bronwyn M. 
Published: August 2023

Abstract
Overview
Reproductive experience (pregnancy and motherhood) leads to long-term changes in the neurobiological and hormonal features of anxiety in rats and humans. The aim of this study was to examine whether reproductive experience alters the effects of two pharmacological treatments for anxiety, a benzodiazepine (diazepam) and a selective serotonin reuptake inhibitor (fluoxetine), on animal models of anxiety.

Methods
In Experiment 1, virgin (n = 47) and age-matched mother (n = 50) rats at 1-month post-weaning were injected with diazepam (1.3 mg/kg or 1.7 mg/kg, i.p.) or vehicle, in the proestrus (high estradiol/progesterone/allopregnanolone) or metestrus (low estradiol/progesterone/allopregnanolone) phase of the estrous cycle 30 min prior to the elevated plus maze (EPM). In Experiment 2, virgin (n = 25) and mother rats (n = 20) were administered fluoxetine (10 mg/kg) or vehicle for 2 weeks prior to being tested on a Pavlovian fear conditioning and extinction protocol, and the EPM.

Results
Replicating past research, in virgin rats, the low dose of diazepam produced anxiolytic-like effects in proestrus, but only the high dose was anxiolytic-like in metestrus. In contrast, in mother rats, both doses of diazepam were anxiolytic-like irrespective of estrous phase. Fluoxetine produced anxiogenic-like effects in virgin rats during fear extinction and the EPM, but had no behavioural effects in mothers. In contrast, fluoxetine increased plasma corticosterone levels measured 30-min post-EPM in mothers, but not virgin rats.

Conclusions
Reproductive experience alters the dose responsivity and efficacy of common anti-anxiety medications in female rats. These findings highlight the importance of considering reproductive status in studies on anxiety and its treatment.
Association between alcohol and urolithiasis: a mendelian randomization study
Authors: Yang Shijian , Tan Wenyue , Wei Baian , Gu Chiming , Li Siyi , Wang Shusheng 
Published: August 2023

AbstractThe causal relationship between alcohol and urolithiasis remains uncertain, despite previous observational studies reporting an association between the two. To determine the causality, we conducted a two-sample Mendelian randomization (MR) analysis. In this study, we aimed to investigate the causal relationship between alcohol and kidney stones using a two-sample MR approach. Two sets of genetic instruments were utilized in the analysis, both of which were derived from publicly available genetic summary data. The first set consisted of 73 single-nucleotide polymorphisms (SNPs) robustly linked to alcohol intake frequency (AIF) and the second set was comprised of 69 SNPs associated with alcohol consumption (AC). Our MR analysis was performed using several methods including the inverse-variance weighted (IVW) method, weighted median method, MR-Egger regression, MR Pleiotropy RESidual Sum and Outlier test. Our results from the MR analysis revealed a borderline significant association between AIF and the risk of urolithiasis. This was established through the use of the IVW method (OR (95% CI) = 1.29 (1.02, 1.65), p = 0.036) and the weighted median approach (OR (95% CI) = 1.44 (1.10, 1.89), p = 0.008). The MR-Egger model also yielded similar risk estimates (OR (95% CI) = 1.39 (0.66, 2.93), p = 0.386), although the relationship was not statistically significant. Sixty-eight SNPs were identified as having a substantial and independent link with AC. However, the IVW approach revealed no significant effect of AC on the risk of urolithiasis (OR (95% CI) = 0.74 (0.48, 1.14), p = 0.173). The MR analysis suggested a potential causal association between alcohol intake frequency and the risk of urolithiasis, but not alcohol consumption.
Impact of Topical Epinephrine with Xylocaine on Drainage After Axillary Lymph Node Dissection: A Randomized Controlled Trial
Authors: Dehariya Prerna , Agarwal Namrata , Yadav Sanjay Kumar , Agarwal Pawan , Sharma Dhananjaya 
Published: August 2023

AbstractIntroductionWe evaluated the impact of topical epinephrine with xylocaine on drainage after axillary lymph node dissection.MethodsFifty‐two women were randomized into two groups, epinephrine with xylocaine drainage (ED) and conventional drainage group (CD). The amount of drainage, duration of drainage catheter, incidence of seroma formation and wound infection were noted.ResultsThe mean total drainage volume was less in ED group as compared to CD group (195 ml vs. 353 ml; p = .002). Drainage catheter was removed earlier in ED group as compared to CD group (5.07 + / − 0.99 days vs. 6.65 + / − 1.07 days; p = 0.0001). The incidence of seroma and wound infection was similar in two groups.ConclusionTopical epinephrine with xylocaine after axillary lymph node dissection results in significantly decreased drainage volume and duration of drainage.