bluuebunny/crossref_metadata_2025_split huggingface dataset:
A fungal pathogen manipulates phytocytokine signaling for plant infection
Authors: Nuernberger Thorsten , Hua Chenlei , Zhang Lisha , Stintzi Annick , Schaller Andreas , Guo Hui-Shan 
Published: July 2023

Abstract
Phytocytokines are hormone-like plant peptides that modulate immune homeostasis and development. Phytosulfokine (PSK) mediates plant growth and attenuates activation of plant pattern-triggered immunity (PTI). We show that the small cysteine-containing effector VdSCP8 from Verticillium dahliae (Vd) is a virulence-promoting protein that suppresses PTI in Arabidopsis thaliana and Nicotiana benthamiana. Apoplastic SCP8 suppresses immune activation through leucine-rich repeat ectodomain pattern recognition receptors. SCP8 virulence and immunosuppressive activities require PHYTOSULFOKINE RECEPTOR 1 (PSKR1), which binds PSK and forms a complex with co-receptor BAK1 for PTI suppression. We find that PSK, like SCP8, suppresses PTI, SCP8 stimulates PSKR1-BAK1 complex formation, and that Vd requires PSK signaling for host infection. SCP8 interacts with an apoplastic subtilase, and co-expression of SCP8 and subtilase inhibitors reduces PTI suppression. Our findings suggest that a multi-host plant pathogen manipulates PTI by enhancing immunosuppressive PSK signaling, likely through plant subtilase activity.
Availability of psychological resources for parents receiving a communication of positivity at newborn screening for metabolic diseases in Italy
Authors: Bani Marco , Caviglia Stefania , Bensi Giulia , Carcereri Mirsada Sarah , Greco Benedetta , Lastrucci Elisa , Massa Pamela , Vissani Sofia , Cazzorla Chiara 
Published: July 2023

Abstract
Receiving communication of positivity at the expanded newborn screening (ENBS) for metabolic diseases is a stressful event in the case of confirmation of positive or false-positive cases. The availability of psychological support to families is crucial across the different communication steps and is recommended by different guidelines and position papers. However, little is known about the availability of psychological resources in the ENBS process.
This national survey aimed to provide an overview of the availability of psychological resources for parents who received communication of positivity at the ENBS in the 23 Italian centers and how the support is provided to parents.
An online survey was sent to the Heads of the ENBS centers asking about the availability of a clinical psychologist, their involvement in the ENBS process and an estimation of parents receiving psychological support.
Nearly 60% of the centers report having a clinical psychologist in the ENBS team; however, in more than 50% of cases the psychologist does not participate in the consultation with parents (nor for the first consultation post-positivity or at confirmation of diagnosis). Furthermore, nearly 60% of the centers reported that parents sometimes rejected the proposal for psychological sessions.
Conclusions
There is a need for harmonization among the Italian ENBS centers with regard to the availability of psychological resources and how these resources are provided to families. Parents’ needs remained only partially fulfilled.
Cyclic di-GMP rescues H-NS–mediated silencing of bacterial type VI secretion systems
Authors: Zhang Lei , Li Shuyu , Liu Qinmeng , Duan Chongyi , Li Jialin , Sun Hengxi , Xu Lei , Yang Qiao , Wang Yao , Shen Xihui 
Published: July 2023

Abstract

The type VI secretion system (T6SS) is a widespread versatile machine that is encoded by many gram-negative bacteria and plays crucial roles in interbacterial competition and bacteria-host interactions. Histone-like nucleoid structuring protein (H-NS) is a global regulator that represses the expression of T6SS genes in various pathogens and environmental isolates. Bacteria appear to have evolved regulatory mechanisms to relieve the transcriptional repression mediated by H-NS when the T6SS activity is required, but the underlying molecular mechanism awaits elucidation. Here we show that elevated intracellular cyclic di-GMP (c-di-GMP) levels alleviate the H-NS–mediated repression of the T6SS inSalmonella entericaserovar Typhimurium.Mechanistically, c-di-GMP directly binds to the H-NS protein to abrogate its binding to the T6SS promoters, thus derepressing expression of the T6SS genes. Furthermore, we provide evidence that bile salts-induced increase in intracellular c-di-GMP levels leads to activation of theS. TyphimuriumT6SS within the host gut, facilitating its killing of commensal bacteria and successful colonization. The observations that c-di-GMP derepresses gene expression via targeting the H-NS or H-NS-like proteins inVibrio parahaemolyticus,Pseudomonas aeruginosaandPseudomonas putidasuggest a general mechanism through which the H-NS family of proteins act as environment-sensing regulators in Gram-negative bacteria.
Kindergarten Teachers’ Mindfulness in Teaching and Job Satisfaction: A Moderated Mediation Model
Authors: Yin Pan , Huang Chao , Yin Xiaochun , Yang Fengwan , Qiu Shi , Song Daixue 
Published: July 2023

Abstract
Objectives Based on the theory of meaning construction, this research aims to examine the relationship between mindfulness-based instruction and job satisfaction among educators working in kindergarten settings. Specifically, the study will evaluate whether teachers’ self-efficacy mediates this relationship and also explore the potential moderating impact of role maladjustment on this mediation process.
Method A total of 531 kindergarten teachers (Mage = 26.89, 89.64% female) took part in the study by responding to self-administered questionnaires measuring their mindfulness in teaching, teachers’ self-efficacy, role maladjustment, and job satisfaction. The findings of the study indicate that there is a notable and favorable correlation between the practice of mindfulness in teaching and job satisfaction among kindergarten teachers. Furthermore, the analysis suggests that teachers' self-efficacy partially mediates this relationship. These results remained significant even after controlling for demographic variables. Further examination using moderated mediation analysis indicated that role maladjustment did not have a moderating effect on either the direct or indirect links between mindfulness in teaching and job satisfaction.
Conclusions The results highlight the importance of teacher self-efficacy as a mediating factor, emphasizing the role of mindfulness in assisting preschool educators in effectively managing challenges and adversities. This, in turn, enhances emotional regulation and coping skills, while cultivating a heightened awareness of personal strengths and abilities, ultimately resulting in higher job satisfaction.
Histological evaluation of edentulous alveolar ridge horizontal bone augmentations using a xenogeneic bone substitute and autologous platelet concentrates: A prospective case series
Authors: Nagy Pal , Nemeth Florina , Ghanaati Shahram , Heselich Anja , Windisch Peter 
Published: July 2023

Abstract
Purpose:
This case series aimed to assess the efficacy of a novel horizontal ridge augmentation modality using histomorphometry. Combinations of “sticky bone” and tenting screws without autologous bone were used as augmentative materials.
Methods:
Five individuals presenting healed, atrophic, partially edentulous sites that required horizontal bone augmentation before implant placement were enrolled. Patients underwent the same augmentation type and five months of postoperative reentry procedures. The first surgery served as implant site development, while the biopsy and corresponding implant placement were performed during reentry. The bone was qualitatively analyzed using histology and histomorphometry and quantitatively evaluated using cone-beam computed tomography (CBCT).
Results:
Four individuals healed uneventfully. Early wound dehiscence occurred in one case. Histology showed favorable bone substitute incorporation into the newly formed bone and intimate contact between de novo bone and graft material in most cases. Histomorphometry reveled an average of 48% newly formed bone, 19% graft material, and 33% soft tissue components. The CBCT-based mean alveolar ridge horizontal increase was 3.9 mm at five months postoperatively.
Conclusions:
The described augmentation method appears suitable for implant site development resulting in favorable bone quality according to histology. The presence of 48% de novo bone and 33% connective tissue formation on average is highly preferable. However, clinicians must accommodate 1–2 mm resorption in augmentative material width at the buccal aspect, which might indicate a secondary lateral augmentation during implant placement.
Trial registration: ClinicalTrials.gov, ID: NCT05612737. Registered 30 October 2022- Retrospectively registered, https://register.clinicaltrials.gov/prs/app/template/EditRecord.vm?epmode=Edit&listmode=Edit&uid=U0005NHB&ts=6&sid=S000CMHA&cx=-bgh60i
Characteristics of circulating immune cells in HBV-related acute- on-chronic liver failure following artificial liver treatment
Authors: Ju Tao , Jiang Daixi , Zhong Chengli , Zhang Huafen , Huang Yandi , Zhu Chunxia , Yang Shigui , Yan Dong 
Published: July 2023

Abstract
Background and aim:
Liver failure, which is predominantly caused by hepatitis B (HBV) can be improved by an artificial liver support system (ALSS). This study investigated the phenotypic heterogeneity of immunocytes in patients with HBV-related acute-on-chronic liver failure (HBV-ACLF) before and after ALSS therapy.
Methods
A total of 22 patients with HBV-ACLF who received ALSS therapy were included in the study. Demographic and laboratory data were collected and analyzed during hospitalization. Immunological features of peripheral blood in the patients before and after ALSS were detected by mass cytometry analyses.
Results
Patients with Grade I according to the ACLF Research Consortium score were considered to have recovered. In total, 12 patients recovered and 10 patients did not. According to the immunological features data after ALSS, the proportion of circulating monocytes was significantly higher in unrecovered patients, but there were fewer γδT cells compared with those in recovered patients. Characterization of 37 cell clusters revealed that the frequency of effector CD8+ T (P = 0.003), CD4+ TCM (P = 0.033), CD4+ TEM (P = 0.039), and inhibitory natural killer (NK) cells (P = 0.029) decreased in HBV-ACLF patients after ALSS therapy. Sub group analyses after treatment showed that the recovered patients had higher proportions of CD4+ TCM (P = 0.010), CD4+ TEM (P = 0.021), and γδT cells (P = 0.003) and a lower proportion of monocytes (P = 0.012) compared with the unrecovered patients.
Conclusions
Changes in effector CD8+ T cells, effector and memory CD4+ T cells, and inhibitory NK cells are associated with ALSS treatment of HBV-ACLF. Moreover, monocytes and γδT cells exhibited the main differences when patients obtained different prognoses. The phenotypic heterogeneity of lymphocytes and monocytes may contribute to the prognosis of ALSS and future immunotherapy strategies.
Magnetic Incremental Permeability of Elastically Deformed Iron and Nickel
Authors: Stashkov Alexey , Schapova Elizaveta , Nichipuruk Aleksander , Stolbovsky Alexey 
Published: July 2023

Abstract
The field dependences of the magnetic incremental permeability U~(H) have been measured on nickel and iron subjected to both elastic compression and tension. The inflections and additional maxima on the U~(H) curves are observed for nickel under tension and for iron under compression. The appearance of the features on the U~(H) curves is traceable to the induction of magnetic texture of the "easy-plane" type caused by elastic deformation of nickel and iron samples. These features appear only if the signs of magnetostriction and applied load are opposite. Only in this case, there is the possibility of estimation of applied mechanical stresses and residual stresses (after annealing). This can be useful for technical diagnostics of objects made of ferromagnetic materials with different signs of magnetostriction. The proposed technique is makes it possible to estimate the “easy-axis” magnetostriction constant.
The evaluation of resistance of Luffa cylindrica genotypes to the fungus Verticillium dahliae
Authors: Shirzaei Fatemeh , Keykhasaber Mojtaba , Pirnia Mahdi , Rafiei Vahideh , Karimi-Jashni Mansoor 
Published: July 2023

Abstract

Verticillium wilt, caused by V. dahliae is one of the important soil-borne disease on various host plants. Luffa cylindrica, a valuable tropical plant from the Cucurbitaceae, is one of the plants that can be affected by V. dahliae. This research investigated the resistance of several indigenous and non-indigenous genotypes of the Luffa plant to Verticillium wilt disease. To achieve this, the rate of disease severity index (DI) and the area under the disease progression curve (AUDPC) were evaluated in each of the genotypes after infection. The statistical analysis of the data was performed using Excel software. To confirm the proper colonization of plants, a plating assay was conducted on PDA culture. Additionally, to confirm the colonization and quantification of fungus in plant tissue at molecular level, the real-time PCR and specific ITS primers were utilized. The results indicated that all studied Luffa genotypes were categorized into two groups, super sensitive and sensitive, based on the severity of disease development. However, the Angled genotype exhibited less sensitivity to V. dahliae compared to the other studied genotypes, with a disease index of 18%. The results of plating assay and real-time PCR, confirmed the pathogenic effect of the V. dahliae on the luffa plant. However, due to the lack of sufficient studies on the genetics of resistance to Verticillium in Luffa, more extensive and detailed studies at the molecular level are needed to investigate the defense structure of this plant, especially the Angled genotype.
Causality between stock indices and cryptocurrencies during the Russia-Ukraine war
Authors: Mgadmi Nidhal 
Published: July 2023

Abstract
This article examines the causal relationship between stock indices and cryptocurrencies during the current war between Russia and Ukraine. The econometric investigation runs from February 24, 2022 to April 12, 2023, focusing on seven stock market indices (S&P500, DAX, CAC40, Nikkei, TSX, MOEX and PFTS) and seven cryptocurrencies (Bitcoin, Ethereum, Litcoin, Dash, Ripple, DigiByte and XEM). In this article, we try to understand how investors react to fluctuations in financial assets to seek safe havens in crypto currencies. We used dynamic causality in the Granger (1969) sense to detect a possible causal relationship in the short term, and seven models to estimate the long-term relationship between cryptocurrencies and financial assets. The causal relationship between financial market indexes and cryptocurrency coins in the short run indicate that three famous cryptocurrencies (BITCOIN, ETHEREUM, RIPPLE) and the two digital asset with minor popularity (XEM, Digibyte) are impacted by the German, Russian and Ukrainian stock markets. In the long-run we found a positive and significate effect of the American, Canadian, French and Ukrainian stock market indexes on Bitcoin. Thus, the stability of the traditional financial markets during the current war period can be explained on the one hand by investors’ fears of an unstable business climate, and on the other hand, by speculators’ sentiment towards new electronic products which are perceived as hedging instruments and a safe haven in the face of the conflict between Ukraine and Russia.
JEL Classifcation: C5 · C22 · G1
Importance of the posterior plate in three-column tibial plateau fractures: Finite element analysis and clinical validation
Authors: Liu Chen-dong , Hu Sun-jun , Chang Shi-Min , Du Shou-chao , Xiong Wen-feng 
Published: July 2023

Abstract
Background
The medial and lateral dual-plates fixation is considered as the standard method for most bicondylar tibial plateau fractures. Nevertheless, with the concept of the three-column developed, the importance of the anatomical reduction of the posterior column has been stressed. By recent clinical studies, it was advocated the fixation should direct at each sub-column, but it has not been proven by the experimental validation so far.
Methods
Finite element analysis and clinical case series study were contained in this paper. In finite element (FE) study, three experimental models were established: longitudinal triple-plates group (LTPG), oblique triple-plates group (OTPG) and dual-plates group (DPG). Three loading scenarios of a 60kg adult in the physiological walking gait were performed. The distribution of the deformation and the equivalent von Mises stress (VMS) in each structure was calculated for comparison. The comparative measurements including the maximum posterior column collapse (MPCC), the maximum total displacement of the model (MTD), the maximum VMS of cortical posterior column (MPC-VMS) and the maximum VMS located on each group of plates and screws (MPS-VMS).The clinical study evaluated the indicators between the groups with or without the posterior plate, including operation time, blood loss volume, full-weight bearing period, Hospital for Special Surgery Knee Scoring system (HSS), Rasmussen score and common postoperative complications.
Results
In the FE analysis, under all loading conditions, the MPCC was found significant less in LTPG and OTPG compared with DPG (reduced at least 38%), and the difference between the LTPG and OTPG was not significant. The largest MPC-VMS was found in DPG (reduced by 14% in LTPG and 2% in OTPG). The largest MPS-VMS was found in LTPG, followed by OTPG, and the smallest group was DPG, 40% increment was found in LTPG and 19% in OTPG at most. The MTD was found significant less in LTPG and OTPG (reduced by 35% and 42% at most in LTPG and OTPG). In the clinical study, 35 cases were included (14 cases in triple-plates group and 21 cases in dual-plates group). Among them, the operation time was 115.6 minutes and 100.5 minutes in the triple-plates group and the dual-plates (P < 0.05), the blood loss volume was 287.0 ml and 206.6 ml (P < 0.05), the full-weight bearing period was 14.5 weeks and 16.2 weeks (P < 0.05). At the final follow-up, the HSS score was 85.0 in the triple-plates group and 77.5 in the dual-plates (P < 0.05), the Rasmussen score was 24.1 and 21.6 (P < 0.05), there was one case superficial incision infection in triple-plates group.
Conclusion
The application of the supplementary posterior plate was beneficial to optimize the biomechanical stability and functional outcome in the three-column tibial plateau fractures.
Macrokinetics of the Deterioration of Cement-based Grouting Material for Predicting Its Service Life Under Acid Rain Attack
Authors: Hu Wenjing , Fang Shiqiang , Xie Lina , Chen Xueqiang , Zhang Bingjian 
Published: July 2023

Abstract
Cement-based grouting material is widely used to reinforce the unsafe stone inscription although it is considered inappropriate in protection of ancient sites. As this approach is irreversible, a longer service life is desirable for the grouting material and methods to predict the service life are needed. Here we propose a universal formula based on the dissolved amount of calcium ion to predict the deterioration rate of cement-based grouting material caused by acid deposition in the laboratory. The experimental results showed that the dissolved amount of calcium ion had a linear relationship with lnT and the pH value, with better accuracy when the pH value was greater than 3.0. This work could provide a facile and quantitative method to help us predict the service life of cement-based grouting material under acid rain attack outdoors.
Identification of immune-related lncRNAs in peripheral blood of sepsis by transcriptome sequencing and analysis core lncRNA PRKCQ-AS1 roles in prognosis and progression of sepsis
Authors: Ding Xian , Liang Wenqi , Xia Hongjuan , Liu Yuee , Liu Shuxiong , Xia Xinyu , Zhu Xiaoli , Pei Yongyan , Zhang Dewen 
Published: July 2023

Abstract
Sepsis is a high mortality and great harm systemic inflammatory response syndrome caused by infection. lncRNAs are potential prognostic marker and therapeutic target. Therefore, we expect to screen and analyze lncRNAs with potential prognostic markers in sepsis. We obtained 2310 differentially expressed (DE) lncRNAs and 7310 DEmRNAs by transcriptome sequencing. Then the immune-related lncRNA-mRNA regulatory network, which contains 14 core lncRNAs, was constructed by functional enrichment and Pearson correlation analysis. The results of immune infiltration, gene set enrichment analysis (GSEA) and gene set variation analysis (GSVA) further confirmed the correlation between 14 key lncRNAs and immune cells. Subsequently, the key immune-related lncRNA PRKCQ-AS1 was identified by weighted gene co-expression network analysis (WGCNA). RT-qPCR showed that PRKCQ-AS1 was up-regulated in clinical samples and sepsis model cells (LPS-induced HUVECs). Kaplan-Meier (KM), receiver operator characteristic (ROC), Cox regression analysis and nomogram confirmed that PRKCQ-AS1 was an independent prognostic factor in sepsis patients. Immune correlation analysis showed that PRKCQ-AS1 was involved in the immune response and inflammatory process of sepsis. Cell function assay confirmed that PRKCQ-AS1 could inhibit the LPS-induced sepsis model cells viability and promote cell apoptosis, inflammatory damage and oxidative stress. In conclusion, we constructed immune-related lncRNA-mRNA regulatory networks in the progression of sepsis and analyzed the role of PRKCQ-AS1 in the prognosis and progression of sepsis. It is confirmed that PRKCQ-AS1 is an important prognostic factor affecting the progression of sepsis and is involved in immune response.
Functional Network Characteristics in Adolescent Psychotic Mood Disorder: Associations with Symptom Severity and Treatment Effects
Authors: Zhou Jingshuai , Duan Jia , Liu Xiaoxue , Wang Yang , Zheng Junjie , Tang Lili , Zhao Pengfei , Zhang Xizhe , Zhu Rongxin , Wang Fei 
Published: July 2023

Abstract
Introduction:
Adolescent psychotic mood disorder (MDP) is a specific phenotype that characterized by more severe symptoms and prognosis compared to nonpsychotic mood disorder (MDNP). But the underlying mechanisms remain unknown, and graph theory analysis can help to understand possible mechanisms of psychotic symptoms from the perspective of functional networks.
Methods
A total of 177 adolescent patients with mood disorders were recruited, including 61 MDP and 116 MDNP. Functional networks were constructed, and topological properties were compared between the two groups at baseline and after treatment, and the association between properties changes and symptom improvement was explored.
Results
Compared to the MDNP group, the MDP group exhibited higher small-world properties (FDR q = 0.003) and normalized clustering coefficients (FDR q = 0.008) but demonstrated decreased nodal properties in the superior temporal gyrus (STG), Heschl’s gyrus, and medial cingulate gyrus (all FDR q < 0.05). These properties were found to be correlated with the severity of psychotic symptoms. Topological properties also changed with improvement of psychotic symptoms after treatment, and changes in degree centrality of STG in the MDP was significantly positive correlated with improvement of psychotic symptoms (r = 0.377, P = 0.031).
Conclusions
This study indicated that functional networks are more severely impaired in patients with psychotic symptoms. Topological properties, particularly those associated with the STG, hold promise as emerging metrics for assessing symptoms and treatment efficacy in patients with psychotic symptoms.