bluuebunny/crossref_metadata_2025_split huggingface dataset:
The Efficacy of Ocular Lubricants With 0.38% SH Gelling Ingredient and 0.18% SH Non-gelling Ingredient in Treating Dry Eye in Adult Gazan Participants
Authors: Aljarousha Mohammed , Badarudin Noor Ezailina , Azemin Mohd Zulfaezal Che , Aljeesh Yousef , Amer Abuimara , Rahim Muhammad Afzam Shah Abdul 
Published: September 2023

Abstract

Purpose: This study aimed to assess the efficacy of two formulations of lubricant eye drops, containing a gelling agent or not, compared to normal saline.
Study Design: This was a prospective, randomized, double-blinded, three-group, parallel, interventional single-site clinical study.
Methods: Forty-five Gazan participants with moderate to severe dry eye disease (DED) were randomized into three groups of 15 participants each. Each group received either normal saline eye drops or lubricant eye drops. For each group, one drop was applied three times a day for six weeks. All participants applied the normal saline solution for the first week. The outcomes assessed were the Arab-ocular surface disease index (OSDI) scores and clinical tests including tear break-up time test (TBUT), corneal fluorescein staining (CFS), and lissamine green conjunctival staining (LGS) at weeks 1, 3, and 6.
Results: Both formulations exhibited a significant improvement in Arab-OSDI scores from visit 2 at follow-up time points (p < 0.001). TBUT, CFS, and LGS showed an improvement in both the 0.15% SH and 0.38% SH groups (p < 0.05). SH 0.38% had a greater improvement in the proportion of evaporative dry eye from visit 2 to visit 5 (p = 0.001).
Conclusion: Lubricant eye drops are beneficial for alleviating the symptoms of dry eye. There was no noticeable difference in the effectiveness of these formulations in relieving symptoms and changing any of the objective signs that were assessed. Improved EDE outcomes occurred with SH 0.38% eye drops, observed between visit 2 and visit 5.
Identification of circRNA-miRNA-mRNA regulatory network associated to the autism spectrum disorder in children through integrated bioinformatics analysis
Authors: Pour Najmeh Nezamabadi , Ahmadi Kambiz , Ebrahimi Seyed Omar , Reiisi Somayeh , Jahanara Abbas 
Published: September 2023

Abstract
Background: Autism spectrum disorder (ASD) is a complex neurological disability with multifactorial etiology. ASD is described by behavior, speech, language, and communication defects. CircRNA is a type of ceRNA that plays an important role in modulating microRNAs (miRNA) in several disorders. However, the potential role of the circRNA/miRNA/mRNA regulatory network in the pathogenesis of ASD is not fully understood. Therefore, this study aimed to create a circRNA/miRNA/mRNA network associated with ASD to cast light on the pathogenesis of ASD.
Methods: CircRNA expression profile data were recruited from Gene Expression Omnibus datasets, and the differentially expressed circRNAs (DEcircRNAs) were identified. Then, miRNAs modulated by these circRNAs were predicted and overlapped with differentially expressed miRNAs. Next, the potentially involved genes were identified by overlapping predicted targets, and differentially expressed genes. The enrichment analysis was performed, and a PPI network was projected. Subsequently, ten key genes were selected from the network. Furthermore, a circRNA/miRNA/mRNA regulatory network was constructed, and probable molecules and drugs with potential anti-ASD effects were predicted.
Results: 11 DEcircRNAs and 8 miRNAs regulated by 4 circRNAs were identified as being significantly involved. Subsequently, gene enrichment analysis of 71 overlapped mRNA regulated by these miRNAs showed that they are mostly associated with hippocampal synaptogenesis, neurogenesis, and axon guidance. Additionally, two high-score compounds, GSK3β inhibitor (SB216763) and dexamethasone, and three drugs (haloperidol, nystatin, paroxetine) were confirmed as potential therapeutic options for ASD.
Conclusion: The results of this study may help gain deeper insight into the pathogenesis of the circRNA/miRNA/mRNA regulatory network in ASD, providing potential therapeutic management options.
A protease from Moringa oleifera Lam exhibits plasmin like activity of blood clot solubilization under ex-vivo and in-vitro hydrolysis of fibrin
Authors: Sawetaji Sawetaji , Aggarwal Kamal Krishan 
Published: September 2023

Abstract

Thrombosis is the formation of abnormal blood clots in the blood vessels that obstruct blood flow and lead to cause thrombosis. Current treatments for thrombosis are associated with serious side effects. Therefore there is a need for alternative natural therapy. To isolate and characterize fibrinolytic protease from M.oleifera and evaluation of its fibrinolytic efficiency. Fresh leaves of Moringa oleifera Lam were taken, fibrinolytic protease was isolated and characterized for its potential to solubilize fibrin under in-vitro conditions and its blood clot solubilization efficiency under ex-vivo experiments. The isolated protease showed a single protein band on native-PAGE. It showed optimum fibrinolytic activity at pH 8.0, 37 oC at 50µg concentration. Its fibrinolytic activity was also confirmed by fibrin zymography. Km and Vmax of isolated protease was determined by the Lineweaver Burk plot. The isolated protease could solubilize 96.41% of blood clot by 96 hrs under ex-vivo conditions. In-vitro fibrin hydrolysis and ex-vivo blood clot solubilization activities shown by isolated protease from leaves of Moringa oleifera Lam suggest its fibrinolytic potential to dissolve blood clots. Being a natural molecule and from a dietary plant it can be explored as an alternative natural therapy against thrombosis.
Large-scale genome-centric metagenomics reveals the prevalence of lysogenic virus-host interactions in activated sludge
Authors: Zhang Xu-Xiang , Li Jie , Wang Depeng , He Xiwei , Liu Peng , Ren Hongqiang 
Published: September 2023

Abstract

Bacteriophages are the most common organisms in the activated sludge (AS) of wastewater treatment plants (WWTPs), playing an important role in maintaining or regulating the microbial community. However, lysogenic virus-host interactions in artificial AS systems remain poorly understood. To comprehensively characterize integrated prophages in AS, we reconstructed metagenome-assembled genomes (MAGs) from 44 full-scale WWTPs across five countries. Results showed that lysogenic virus-host interactions were prevalent in AS systems, with > 55% lysogenic MAGs (lysogens), and Proteobacteria (13.8%) and Bacteroidota (12.7%) were the most common lysogenic bacterial phyla. Gene-sharing network analysis revealed that most prophages (~ 99%) could not be categorized into the recognized viral genera, indicating that AS is an untapped source of novel and diverse viruses. The community structure and diversity of lysogens and parasitic prophages in AS systems showed evidently spatial variations, but AS systems from different locations possessed common functional lysogenic microorganisms. In addition, the parasitic prophages detected within lysogens were active and inducible. The active prophages were found to be able to lyse the microorganisms involved in the removal of organic matter, nitrogen, and phosphorous. To our best knowledge, this is the first study to reveal the predominance of lysogenic virus-host interactions in AS systems by large-scale genome-centric metagenomics, which enhanced our understanding of the pivotal role of the prophages in the AS microbiome.
SIRT1 inhibits Smad3 acetylation by reducing Lnc CRNDE transcription and inhibits BV2 cell overactivation to promote SCI neurological repair
Authors: Chen Hongbo , Li Qiaofen , Yu Huimin , Pan Zhipeng , Zhang Bingxue , Yan Na , Zhang Yuying , Li Guiliang , Lv Shaokun , Lv Meifen 
Published: September 2023

Abstract
Methods
We established a BV2 cell activation model by in vitro lipopolysaccharide (LPS) treatment and a rat SCI model according to physical injury. We used HE staining, Luxol solid blue staining and Nissl staining to observe the spinal cord structure, RT‒qPCR to detect SIRT1 mRNA and Lnc CRNDE expression, western blotting to detect SIRT1 protein, BV2 cell activation protein marker (Iba-1) and autophagy-related protein (LC3; Beclin-1; P62) expression, immunoprecipitation reaction to detect the relationship between Smad3 and SIRT1 binding, RNA binding protein immunoprecipitation (RIP) to detect the relationship between Smad3 and CRNDE, dual luciferase reporter gene to verify the transcriptional regulation of CRNDE by Smad3, and immunofluorescence staining to detect the coexpression of BV2 cell activation marker (Iba-1) and autophagy marker (P62).
Results
SIRT1 was expressed at low levels in SCI and LPS-treated BV2 cells from SCI rats. Overexpression of SIRT1 promoted BV2 cell autophagy, inhibited BV2 cell overactivation, alleviated the pathological conditions of spinal cord congestion, edema and structural damage after SCI, improved BBB scores, increased neuronal numbers and promoted myelin regeneration. SIRT1 could inhibit Lnc CRNDE transcription by reducing Smad3. SIRT1 inhibits Lnc CRNDE transcription by reducing Smad3 acetylation and inhibiting its nuclear localization. Overexpression of CRNDE reversed the protective effect on SCI exhibited by SIRT1, and knockdown of CRNDE inhibited BV2 cell overactivation and promoted SCI repair.
Conclusion
SIRT1 promotes SCI repair by reducing Smad3 acetylation and inhibiting its nuclear localization to suppress Lnc CRNDE transcription and inhibit BV2 cell overactivation.
Ubiquitin-conjugating enzyme 2C (UBE2C) plays as a cancer diagnostic and prognostic marker: A Bioinformatics pan-cancer analysis
Authors: Jalali Pooya , Samei Amir , Rezaie Malihe , Shahmoradi Arvin , Pashizeh Fatemeh , Salehi Zahra 
Published: September 2023

Abstract
Background
The diverse and complex attributes of cancer have made it a daunting challenge to overcome globally and remains to endanger human life. Detection of critical cancer-related gene alterations in solid tumor samples better defines patient diagnosis and prognosis, and indicates what targeted therapies must be administered to improve cancer patients’ outcome.
Materials and Methods
To identify genes which have aberrant expression across different cancer types, differential expressed genes were detected within the TCGA datasets. Subsequently, the DEGs common to all pan cancers were determined. Furthermore, various methods were employed to gain genetic alterations, co-expression genes network and protein-protein interaction (PPI) network, pathway enrichment analysis of common genes. Finally, the gene regulatory network was constructed.
Results
Intersectional analysis identified UBE2C as a common DEG between all 28 types of studied cancers. Upregulated UBE2C expression was significantly correlated with OS and DFS of 10 and 9 types of cancer patients. Also, UBE2C can be a diagnostic factor in CESC, CHOL, GBM, and UCS with AUC = 100% and diagnose 19 cancer types with AUC ≥ 90%. A ceRNA network constructed including UBE2C, 41 TFs, 10 shared miRNAs, and 21 circRNAs and 128 lncRNAs.
Conclusion
In summary, UBE2C can be a theranostic gene which may serve as a reliable biomarker in diagnosing cancers, improving treatment responses and increasing the overall survival of cancer patients and can be a promising gene to be target by cancer drugs in the future.
Complications of Delayed Diagnosis and Challenges: Hereditary Spherocytosis with Hepatocellular Jaundice, a Case Report from Ethiopia
Authors: Mekonnen Sintayehu , Adefris Dereje , Bati Abdi , Azmeraw Daniel , Teshome Eliud , Farris Hawi 
Published: September 2023

Abstract

Hereditary spherocytosis is a rare genetic disorder of the RBC membrane that is characterized by anemia, jaundice, and splenomegaly; however, in the absence of family history and with unusual clinical presentation, the diagnosis might not be made until later in life. Here, we present a challenging case of genetically proven hereditary spherocytosis that involves the SPTB gene in a 23-year-old female patient who had repeated medical visits for episodic jaundice, where the delay in diagnosis led to several complications.
The impact of accounting information quality and financing constraints on innovation input of Chinese private enterprises
Authors: Tang Liqing 
Published: September 2023

Abstract
With the advancement of economic globalization and the gradual intensification of competition in international market, enterprises need to constantly enhance their comprehensive strength to consolidate market position and improve the internal innovation ability, which is also a new mission given to enterprises under the new era. At present, the innovation activities of Chinese enterprises are faced with many difficulties, such as long investment cycle time, too much investment capital, high risk, short-term income is not obvious, and so on. However, if enterprises use agents to promote enterprise innovation, the financing constraints caused by the current market agency costs and internal and external information asymmetry greatly hinder the process of independent innovation of private enterprises. Therefore, it is of great significance to study the influencing factors of private enterprises' input in indigenous innovation to improve their independent innovation ability and promote their further development.
Blockchain and human resource management: Emerging themes and research agenda
Authors: Zirar Araz 
Published: September 2023

Abstract
The potential of Blockchain to solve human resource management (HRM) pain points is increasingly being pitched. This review explores the value of Blockchain in HRM. A systematic search of 622 documents from Scopus, Web of Science, Business Source Complete, ABI/INFORM Collection, and Emerald Insight yielded 25 articles on Blockchain's potential for HRM. The analysis suggests that (1) rather than comparing HR professionals' awareness of blockchain technology against non-HR professionals, it is necessary to inquire about HR professionals' awareness level of the technology; (2) while Blockchain has potential for HRM, the advantages of Blockchain in HRM are often perceived rather than actual, and a convincing rationale for adoption is lacking; (3) the cited contributions of Blockchain to HRM assume a range of assumptions about Blockchain in the cryptocurrency world and reflect a high-level understanding of what Blockchain is or may be; (4) HR professionals and their organisations must examine the likely consequences of blockchain adoption, such as the need for highly sought-after skills, skill distribution between Blockchain and workers, and workers with obsolete skills, and (5) research focus should be on how to deal with the challenges of blockchain adoption rather than the perks that an organisation and HR professionals may gain. Research questions are offered after each theme to encourage further research.
Microstructure and properties of iron-based surfacing layer based on JmatPro software simulation calculation
Authors: Chen Zhongbing , Yu Yunhe , Hou Jixin , Zhu Ping , Zhang Jianlin 
Published: September 2023

The material performance simulation software JMatPro was used to simulate and calculate equilibrium phase structure and properties of a mixture formed by mixing different proportions of Norem02 alloy and F304 stainless steel. The results show that in the range of 500-1300 °C, equilibrium phases of the mixture include Austenite and Ferrite, and the carbides include M2(C,N), M6C, M7C3 and M23C6. As the content of F304 in the mixture increases, the phase transition temperature A1 of the mixture decreases, and A3 first decreases and then increases; in the equilibrium phase, the content of Austenite and Ferrite increases, and the content of carbide decreases until the carbides M7C3 and M6C gradually disappear. Density, coefficient of linear expansion, thermal conductivity, Young’s modulus, Poisson’s ratio and specific heat capacity of different mixture materials vary with temperature in a complex manner. Strength and hardness of the mixture formed when the content of F304 is 20 % is the highest. The finer the grain size of the mixture, the higher the strength and hardness of the material. Comparing standard physical property data of F304 stainless steel at room temperature with the simulation calculation results, it is found that the error is small, which verifies the accuracy of the simulation calculation results.
Development of inertial piezoelectric linear actuator with asymmetric design
Authors: Čeponis Andrius , Jūrėnas Vytautas , Mažeika Dalius , Deltuvienė Dovilė 
Published: September 2023

This article represents a numerical and experimental investigation of a piezoelectric linear actuator with asymmetric design. The actuator is based on a square-shaped rod with asymmetrical cut and T-shaped clamping. The cylindrical rail, with slider, is on the opposite side of the T-shaped clamping. The low-volume and mass of piezoelectric actuator allows to mount it directly to printed circuit board (PCB). The actuator operation is based on the inertial stick – slip operation principle inducted by the first longitudinal vibration mode excited by two sawtooth signals with phase difference π. Furthermore, the longitudinal vibration mode induces bending deformations of the rod, increasing the displacements of the cylindrical rail. The results of numerical and experimental investigations have validated the actuator operating principle, as well as shown that the displacement amplitudes of the guidance rail have reached 153.6 μm at the 200 Vp-p excitation signal amplitude, while the linear motion speed reached 45.6 mm/s at the same excitation signal.
The removal of ammonia-nitrogen from aquaculture water based on cavitation effect of ultrasonic vibration
Authors: Fuyu Guo , Jianguo She , Ning Chen 
Published: September 2023

This paper mainly studies the mechanism and efficiency of ammonia-nitrogen removal from circulating sewage in closed fish tank system. According to some factors affecting the removal efficiency (ultrasonic power, ultrasonic time and water oxygen concentration). Simulated circulating sewage mixed with oxygen through a venturi tube and then entered a closed experiment box for ultrasonic experiments. Finally, the removal efficiency was determined by Knott's reagent photometry. In the experiment, the PH value of the simulated sewage was 7.5, the indoor water temperature was 20 °C, and the free ammonia was selected as 0.02 mg/L (total ammonia-nitrogen was 1.6 mg/L) according to the standard. The experiment found that when the ultrasonic power is 400 W, the action time is 2 h, and the oxygen concentration of water is 23.8 mg/L, the efficiency of removing ammonia-nitrogen in water is about 65 %. When applied on fishing vessels, increasing the power can increase the removal efficiency.
Enunciação e subjetividade
Authors: Santos James Washington Alves dos 
Published: September 2023

Neste artigo, vamos trabalhar as noções de enunciação e subjetividade, a partir do gênero carta, produzida como complemento ao conto de Dalton Trevisan intitulado: “Apelo”. Para este intuito, usamos as respostas ao texto feitas pelos alunos do 1º período do curso de Letras Inglês/Português da UNEAL (3 respostas), que se caracterizam como exercício da disciplina de Leitura e Produção de Texto. Metodologicamente, esta análise se coloca como um caminho a ser trabalhado qualitativamente em sala de aula, a saber: pelo uso de exercícios de complemento textual para leitura de gêneros literários (epopeias, narrativas e poesias líricas), tanto quanto para gêneros discursivos (carta, conto, cordel etc.). Assim, este artigo busca definir teoricamente, dentro do gênero discursivo carta, o que seria a interação na linguagem, produzindo sentido de enunciação, bem como a importância da consciência do ‘eu’ para a produção e a leitura do texto, constituindo a realidade fundamental da linguagem.
The Impact of COVID-19 Pandemic on Public Transport: A Mobility Survey in Naples (South of Italy)
Authors: Henke Ilaria , Pagliara Francesca , Cartenì Armando , Coppola Pierluigi 
Published: September 2023

Introduction:
The COVID-19 outbreak has not only had a fatal impact on people's lives, but it has also threatened the entire world of transportation. The number of trips, the mobility habits and the users’ mode choices from the beginning of the pandemic has changed significantly over the last year.

Objective:
This manuscript analyzed the effects of the COVID-19 pandemic on the users’ mobility choices following the pandemic phases during “new normality” was analysed. The objective of this research is twofold: i) to estimate and evaluate the changes in user's mode choices and environmental impact; ii) to identify mobility policies aimed at fostering the use of local Public Transport (PT).

Methods:
A Stated Preference survey was carried out during the COVID-19 pandemic in the city of Naples (South of Italy): i) to assess users’ mobility habits before the COVID-19 pandemic; ii) to evaluate users’ choice of Public Transport after the COVID-19 pandemic period; iii) to identify the attributes related to users’ reluctance (e.g., fear of contagion, service uncertainty) to continue using public transport and the main characteristics that Public Transport should have to be perceived as a safe transport alternative.

Results:
The results show that 56% of users would be willing to wait up to 20 minutes longer for the bus and train to ensure social distancing, especially on reliable info mobility.

Conclusion:
Moreover, 6% of the users, who previously travelled with local PT, would shift to private transport. This means that in the province of Naples, there would be more than 54 million trips by private car with the subsequent effects on congestion and environmental pollution in one month.
Supporting Clinical Competencies in Men’s Mental Health Using the Men in Mind Practitioner Training Program: User Experience Study
Authors: Seidler Zac E , Benakovic Ruben , Wilson Michael J , Fletcher Justine , Oliffe John L , Owen Jesse , Rice Simon M 
Published: September 2023

Background
Engaging men in psychotherapy is essential in male suicide prevention efforts, yet to date, efforts to upskill mental health practitioners in delivering gender-sensitized therapy for men have been lacking. To address this, we developed Men in Mind, an e-learning training program designed to upskill mental health practitioners in engaging men in therapy.

Objective
This study involves an in-depth analysis of the user experience of the Men in Mind intervention, assessed as part of a randomized controlled trial of the efficacy of the intervention.

Methods
Following completion of the intervention, participants provided qualitative (n=392) and quantitative (n=395) user experience feedback, focused on successes and suggested improvements to the intervention and improvements to their confidence in delivering therapy with specific subpopulations of male clients. We also assessed practitioner learning goals (n=242) and explored the extent to which participants had achieved these goals at follow-up.

Results
Participants valued the inclusion of video demonstrations of skills in action alongside the range of evidence-based content dedicated to improving their insight into the engagement of men in therapy. Suggested improvements most commonly reflected the desire for more or more diverse content, alongside the necessary adaptations to improve the learning and user experience. Participants also commonly reported improved confidence in assisting men with difficulty articulating their emotions in therapy and suicidal men.

Conclusions
The evidence obtained from this study aids in plans to scale Men in Mind and informs the future development of practitioner training interventions in men’s mental health.

International Registered Report Identifier (IRRID)
RR2-10.1186/s40359-022-00875-9