bluuebunny/crossref_metadata_2025_split huggingface dataset:
Up-regulation of CREB-1 regulates tendon adhesion in the injury tendon healing through the CREB-1/TGF-β3 signaling pathway
Authors: Wu Li-Ming , Wang Yun-Jiao , Li Shuai-Feng , Wang Jing-Kun , Liu Jun , Fan Chao-Chao , Xiong Yan 
Published: April 2023

Abstract
Aim
To explore the mechanism of the healing of tendon tissue and anti-adhesion, and to discuss the role of the transforming growth factor-β3 (TGF-β3)/cAMP response element binding protein-1 (CREB-1) signaling pathway in the healing process of tendons.

Method
All mice were divided into four groups of 1, 2, 4, and 8 weeks respectively. Each time group was divided into four treatment groups: the amplification group, the inhibition group, the negative group, and the control group. When the tendon injury model was established, the CREB-1 virus was injected into the tendon injury parts. A series of methods such as gait behaviourism, anatomy, histological examination, immunohistochemical examination and collagen staining were employed to assess the tendon healing and the protein expression of TGF-β3, CREB-1, Smad3/7 and type I/III collagen (COL-I/III). CREB-1 virus was sent to tendon stem cells to assess the protein expression of TGF-β1, TGF-β3, CREB-1, COL-I/III by methods such as immunohistochemistry and Western blot.

Results
The amplification group showed better gait behaviourism than the inhibition group in the healing process. The amplification group also had less adhesion than the negative group. Hematoxylin–eosin (HE) staining of tendon tissue sections showed that the number of fibroblasts in the amplification group was less than the inhibition group, and the immunohistochemical results indicated that the expression of TGF-β3, CREB-1, and Smad7 at each time point was higher than the inhibition group. The expression of COL-I/III and Smad3 in the amplification group was lower than the inhibition group at all time points. The collagen staining indicated that the ratio of type I/III collagen in the amplification group was higher than the negative group at 2,4,8 week. The CREB-1 amplification virus could promote the protein expression of TGF-β3, CREB-1 and inhibit the protein expression of TGF-β1 and COL-I/III in the tendon stem cells.

Conclusion
In the process of tendon injury healing, CREB-1 could promote the secretion of TGF-β3, so as to promote the tendon healing and have the effect of anti-adhesion in tendons. It might provide new intervention targets for anti-adhesion treatment of tendon injuries.
The relationship between nurses’ psychological resilience and job satisfaction during the COVID-19 pandemic: a descriptive-analytical cross-sectional study in Iran
Authors: Shahrbabaki Parvin Mangolian , Abolghaseminejad Parniya , lari Leyla Ahmadi , Zeidabadinejad Somayeh , Dehghan Mahlagha 
Published: April 2023

Abstract
Background
Job satisfaction and factors affecting them are among the most important social issues. Resilience has a moderating role in the relationship between stress and diseases, so it can affect a person's job satisfaction because it enables a person to deal with adverse conditions. This study aimed to investigate the relationship between nurses’ psychological resilience and job satisfaction during the COVID-19 outbreak.

Methods
This descriptive-analytical cross-sectional study (2022) used convenience sampling to select 300 nurses. The Connor and Davidson Resilience Scale and Minnesota Satisfaction Questionnaire were used to collect data. The data were then analyzed with SPSS 22 and statistical methods (Independent t-test, Analysis of Variance, Pearson correlation coefficient, and Multiple linear regression).

Results
The study results showed a positive and poor relationship between resilience, some of its dimensions (trust in individual instincts, tolerance of negative affect (p = 0.006), positive acceptance of change and secure relationships (p = 0.01), spiritual influences (p = 0.04)) and job satisfaction (p < 0.001). In other words, nurses’ high level of resilience increased their job satisfaction and vice versa.

Conclusions
Enhancing the resilience of frontline nurses during the COVID-19 pandemic improved their job satisfaction and affected care provided by them. Nurse managers can control nurses’ resilience and offer interventions that would strengthen it, especially at crises.
Parental help-seeking behaviour for, and care of, a sick or injured child during the COVID-19 pandemic: a European online survey
Authors: Tan Chantal D. , Bressan Silvia , Carter Rachel , Hylén Mia , Kristensson Inger , Lakhanpaul Monica , Mintegi Santiago , Moll Henriette A. , Neill Sarah 
Published: April 2023

Abstract
Background
Globally, the COVID-19 pandemic had a huge impact on patients and healthcare systems. A decline in paediatric visits to healthcare settings was observed, which might have been due to lower incidence of injury and infectious illness, changes in healthcare services and parental concern. The aim of our study was to examine parental experiences of help-seeking for, and care of, a sick or injured child during COVID-19 lockdown periods in five European countries with different healthcare systems in place.

Methods
An online survey for parents with a child with any kind or illness of injury during COVID-19 lockdowns was circulated through social media in five European countries: Italy, Spain, Sweden, the Netherlands, and the United Kingdom. Parents living in one of these countries with self-identification of a sick or injured child during COVID-19 lockdown periods were eligible to fill in the survey. Descriptive statistics were used for the level of restrictions per country, children’s characteristics, family characteristics and reported help-seeking behaviour of parents prior to the lockdown and their real experience during the lockdown. The free text data was subjected to thematic analysis.

Results
The survey was fully completed by 598 parents, ranging from 50 to 198 parents per country, during varying lockdown periods from March 2020 until May 2022. Parents who completed the survey were not deterred from seeking medical help for their sick or injured child during the COVID-19 pandemic. This finding was comparable in five European countries with different healthcare systems in place. Thematic analysis identified three main areas: parental experiences of access to healthcare, changes in parents’ help-seeking behaviours for a sick or injured child during lockdowns, and the impact of caring for a sick or injured child during the lockdowns. Parents reported limited access to non-urgent care services and were anxious about either their child or themselves catching COVID-19.

Conclusion
This insight into parental perspectives of help-seeking behaviour and care for a sick or injured child during COVID-19 lockdowns could inform future strategies to improve access to healthcare, and to provide parents with adequate information concerning when and where to seek help and support during pandemics.
Associations between the use of insecticide-treated nets in early childhood and educational outcomes, marriage and child-bearing in early adulthood: evidence from a 22-year prospective cohort study in Tanzania
Authors: Mrema Sigilbert , Okumu Fredros , Schellenberg Joanna , Fink Günther 
Published: April 2023

Abstract
Background
The effectiveness of insecticide-treated nets (ITNs) in preventing malaria in young children is well established. However, the long-term effects of early childhood ITN use on educational outcomes, fertility, and marriage in early adulthood are not well understood.

Methods
This study uses 22 years of longitudinal data from rural Tanzania to investigate the associations between early life ITN use and educational attainment, fertility and marriage in early adulthood. Unadjusted and adjusted logistic regression models were used to estimate the associations between early life ITN use and early adult outcomes (education, childbearing, and marriage), controlling for potential confounders, such as parental education, household asset quintiles, and year of birth. Analyses were conducted separately for men and women.

Results
A total of 6706 participants born between 1998 and 2000 were enrolled in the study between 1998 and 2003. By 2019 a total of 604 had died and a further 723 could not be found, leaving 5379 participants who were interviewed, among whom complete data were available for 5216. Among women, sleeping under a treated net at least half of the time during early childhood [“high ITN use”] was associated with a 13% increase in the odds of completing primary school (adjusted odds ratio (aOR) 1.13 [0.85, 1.50]) and with a 40% increase in the odds of completing secondary school (aOR 1.40 [1.11, 1.76]) compared with women sleeping less frequently under ITNs in early life (< age 5 years). Among men, high ITN use was associated with a 50% increase in the odds of completing primary school (aOR 1.50 [1.18, 1.92]) and a 56% increase in the odds of completing secondary school (aOR 1.56 [1.16, 2.08]) compared to men with low ITN use in early life. Weaker associations were found between ITN use in early life and both adolescent childbearing (aOR 0.91 [0.75, 1.10]) and early marriage (aOR 0.86 [0.69, 1.05]).

Conclusion
This study found that early life use of ITNs was strongly associated with increased school completion in both men and women. More marginal associations were found between early-life ITN use and both marriage and child-bearing in early adulthood. ITN use during early childhood may have long-term positive effects on educational attainment in Tanzania. However, further research is needed to understand the mechanisms behind these associations and to explore the broader impacts of ITN use on other aspects of early adult life.
Biofilm and wound healing: from bench to bedside
Authors: Goswami Aakansha Giri , Basu Somprakas , Banerjee Tuhina , Shukla Vijay Kumar 
Published: April 2023

AbstractThe bubbling community of microorganisms, consisting of diverse colonies encased in a self-produced protective matrix and playing an essential role in the persistence of infection and antimicrobial resistance, is often referred to as a biofilm. Although apparently indolent, the biofilm involves not only inanimate surfaces but also living tissue, making it truly ubiquitous. The mechanism of biofilm formation, its growth, and the development of resistance are ever-intriguing subjects and are yet to be completely deciphered. Although an abundance of studies in recent years has focused on the various ways to create potential anti-biofilm and antimicrobial therapeutics, a dearth of a clear standard of clinical practice remains, and therefore, there is essentially a need for translating laboratory research to novel bedside anti-biofilm strategies that can provide a better clinical outcome. Of significance, biofilm is responsible for faulty wound healing and wound chronicity. The experimental studies report the prevalence of biofilm in chronic wounds anywhere between 20 and 100%, which makes it a topic of significant concern in wound healing. The ongoing scientific endeavor to comprehensively understand the mechanism of biofilm interaction with wounds and generate standardized anti-biofilm measures which are reproducible in the clinical setting is the challenge of the hour. In this context of “more needs to be done”, we aim to explore various effective and clinically meaningful methods currently available for biofilm management and how these tools can be translated into safe clinical practice.
Genome-wide analysis toward the epigenetic aetiology of myelodysplastic syndrome disease progression and pharmacoepigenomic basis of hypomethylating agents drug treatment response
Authors: Siamoglou Stavroula , Boers Ruben , Koromina Maria , Boers Joachim , Tsironi Anna , Chatzilygeroudi Theodora , Lazaris Vasileios , Verigou Evgenia , Kourakli Alexandra , van IJcken Wilfred F. J. , Gribnau Joost , Symeonidis Argiris , Patrinos George P. 
Published: April 2023

AbstractMyelodysplastic syndromes (MDS) consist of a group of hematological malignancies characterized by ineffective hematopoiesis, cytogenetic abnormalities, and often a high risk of transformation to acute myeloid leukemia (AML). So far, there have been only a very limited number of studies assessing the epigenetics component contributing to the pathophysiology of these disorders, but not a single study assessing this at a genome-wide level. Here, we implemented a generic high throughput epigenomics approach, using methylated DNA sequencing (MeD-seq) of LpnPI digested fragments to identify potential epigenomic targets associated with MDS subtypes. Our results highlighted that PCDHG and ZNF gene families harbor potential epigenomic targets, which have been shown to be differentially methylated in a variety of comparisons between different MDS subtypes. Specifically, CpG islands, transcription start sites and post-transcriptional start sites within ZNF124, ZNF497 and PCDHG family are differentially methylated with fold change above 3,5. Overall, these findings highlight important aspects of the epigenomic component of MDS syndromes pathogenesis and the pharmacoepigenomic basis to the hypomethylating agents drug treatment response, while this generic high throughput whole epigenome sequencing approach could be readily implemented to other genetic diseases with a strong epigenetic component.
The role of emotion-related individual differences in enjoyment and masking smile judgment
Authors: Gallant Adèle , Pelot Annalie , Mazerolle Marie-Pier , Sonier René-Pierre , Roy-Charland Annie 
Published: April 2023

Abstract
Background
While some research indicates that individuals can accurately judge smile authenticity of enjoyment and masking smile expressions, other research suggest modest judgment rates of masking smiles. The current study explored the role of emotion-related individual differences in the judgment of authenticity and recognition of negative emotions in enjoyment and masking smile expressions as a potential explanation for the differences observed.

Methods
Specifically, Experiment 1 investigated the role of emotion contagion (Doherty in J Nonverbal Behav 21:131–154, 1997), emotion intelligence (Schutte et al. in Personality Individ Differ 25:167–177, 1998), and emotion regulation (Gratz and Roemer in J Psychopathol Behav Assess 26:41–54, 2004) in smile authenticity judgment and recognition of negative emotions in masking smiles. Experiment 2 investigated the role of state and trait anxiety (Spielberger et al. in Manual for the state-trait anxiety inventory, Consulting Psychologists Press, Palo Alto, 1983) in smile authenticity judgment and recognition of negative emotions in the same masking smiles. In both experiments, repeated measures ANOVAs were conducted for judgment of authenticity, probability of producing the expected response, for the detection of another emotion, and for emotion recognition. A series of correlations were also calculated between the proportion of expected responses of smile judgement and the scores on the different subscales.

Results
Results of the smile judgment and recognition tasks were replicated in both studies, and echoed results from prior studies of masking smile judgment: participants rated enjoyment smiles as happier than the masking smiles and, of the masking smiles, participants responded “really happy” more often for the angry-eyes masking smiles and more often categorized fear masking smiles as “not really happy”.

Conclusions
Overall, while the emotion-related individual differences used in our study seem to have an impact on recognition of basic emotions in the literature, our study suggest that these traits, except for emotional awareness, do not predict performances on the judgment of complex expressions such as masking smiles. These results provide further information regarding the factors that do and do not contribute to greater judgment of smile authenticity and recognition of negative emotions in masking smiles.
Resource loss, coping strategies and post-traumatic stress disorder symptoms in survivors of the 2020 Croatia earthquake
Authors: Löw Ajana , Lotar Rihtarić Martina , Vrselja Ivana 
Published: April 2023

Abstract
Background
Conservation of resources theory (COR) establishes a link between resource loss and the stress response. The aim of this study was to assess the contribution of resource loss in the form of home damage and the choice of active or passive coping strategies to PTSD symptoms in survivors of the 2020 Petrinja (Croatia) earthquake.

Methods
A total of 374 adults (29.9% men) aged 18–64 years living in the counties surrounding the epicenter of the Petrinja (Croatia) earthquake participated in an online cross-sectional survey. The questionnaire included the PTSD Checklist for DSM-5 (PCL-5), the Coping Inventory, and the binary item assessing whether or not the participants' home was damaged.

Results
Hierarchical regression analysis showed that home damage was a significant predictor of PTSD symptoms. Participants whose homes were damaged by the earthquake were significantly more likely to use passive coping strategies, namely avoidance and emotional venting, and one active coping strategy, action, than those whose homes were spared. Finally, more frequent use of passive coping was associated with a higher risk of PTSD symptoms.

Conclusions
The study corroborates the COR theory link between resource loss and the stress response, as well as the general consensus that passive coping is a less adaptive strategy than active coping. In addition to passive coping, individuals who lacked resources may have been inclined to take some active steps because they either needed to repair or relocate their homes and because most buildings were only moderately to minimally damaged in the Petrinja earthquake.
Mechanisms of trastuzumab induced cardiotoxicity – is exercise a potential treatment?
Authors: Eaton Holden , Timm Kerstin Nina 
Published: April 2023

AbstractThe use of the adjuvant therapeutic antibody trastuzumab in breast cancer is associated with a range of cardiotoxic side effects despite successfully reducing the severity of outcomes cancer patients,. The most common cardiac effect, a reduction in left ventricular ejection fraction (LVEF), is a known precursor to heart failure and often requires interruption of chemotherapy to avoid endangering patients further. An understanding of trastuzumab’s cardiac-specific interactions is therefore critical in devising new methods to not only avoid permanent cardiac damage, but also prolong treatment time, and therefore effectiveness, for breast cancer patients. Increasingly, the use of exercise as a treatment has been indicated across the field of cardio-oncology due to encouraging evidence that it can protect against LVEF reductions and heart failure. This review explores the mechanisms of trastuzumab-mediated cardiotoxicity, as well as the physiological effects of exercise on the heart, in order to assess the suitability of exercise intervention for breast cancer patients on trastuzumab antibody-therapy. We furthermore draw comparison to existing evidence for exercise intervention as a cardioprotective treatment in doxorubicin-induced cardiotoxicity. Although preclinical evidence seems to support exercise-based approaches also in trastuzumab-cardiotoxicity, current clinical evidence is too limited to confidently recommend it as a treatment, largely owing to issues of adherence. Future studies should therefore examine how the variety and duration of exercise can be adjusted to improve treatment effectiveness at a more personalised level.
Pathogenicity of Beauveria bassiana (Balsamo-Crivelli) and Metarhizium anisopliae (Metschnikoff) isolates against life stages of Zeugodacus cucurbitae (Coquillett) (Diptera: Tephritidae)
Authors: Hintènou Micheline V. , Omoloye Adebayo A. , Douro Kpindou Ouorou K. , Karlsson Miriam F. , Djouaka Rousseau , Bokonon-Ganta Aimé H. , Tamȯ Manuele 
Published: April 2023

Abstract
Background
Entomopathogenic fungi are primary pathogens that naturally affect insect pests by suppressing their populations and considered as an ecofriendly agents. The present study aimed to evaluate in vitro activity of different isolates of Beauveria bassiana and Metarhizium anisopliae against the development of larval stages of the Cucurbit fruit fly, Zeugodacus cucurbitae (Coquillett) (Diptera: Tephritidae).

Results
Larval mortality was significantly high with B. bassiana isolate Bb337 (5.82–21.70%) and with the lowest in M. anisopliae isolate MaD (1.49–6.33%). Pupal mortality rate was comparatively higher with more than 50%. The cadavers of all host instars produced conidia (sporulation). Sporulated dead larvae were significantly higher in Bb337 (61.10%) than at the least in MaD (18.60%) at 105 conidia/ml. At 108 conidia/ml, MaD induced the highest pupal cadavers with mycosis (32.42%). Regardless of applied fungal species, host instars mortality significantly increased with increasing concentration of B. bassiana isolates, suggesting a concentration-dependent response of Z. cucurbitae.

Conclusion
The tested isolates demonstrated their pathogenicity through vertical transmission of mycosis from one instar to another, regardless of the concentrations used.
Microparticles: potential new contributors to the pathogenesis of systemic sclerosis?
Authors: de Oliveira Sandra Maximiano , de Azevedo Teixeira Ighor Luiz , França Carolina Nunes , de Oliveira Izar Maria Cristina , Kayser Cristiane 
Published: April 2023

Abstract
Background
Microparticles (MPs) are membrane-derived vesicles released from cells undergoing activation or apoptosis with diverse proinflammatory and prothrombotic activities, that have been implicated in the pathogenesis of systemic sclerosis (SSc). We aimed to evaluate the plasma levels of platelet-derived microparticles (PMPs), endothelial cell-derived microparticles (EMPs), and monocyte-derived microparticles (MMPs) in SSc patients, and the association between MPs and the clinical features of SSc.

Methods
In this cross-sectional study, 70 patients with SSc and 35 age- and sex-matched healthy controls were evaluated. Clinical and nailfold capillaroscopy (NFC) data were obtained from all patients. Plasma levels of PMPs (CD42+/31+), EMPs (CD105+), and MMPs (CD14+) were quantified by flow cytometry.

Results
Patients were mainly females (90%), with a mean age of 48.9 years old. PMP, EMP, and MMP levels were significantly increased in SSc patients compared to controls (79.2% ± 17.3% vs. 71.0% ± 19.8%, p = 0.033; 43.5% ± 8.7% vs. 37.8% ± 10.4%, p = 0.004; and 3.5% ± 1.3% vs. 1.1% ± 0.5%, p < 0.0001, respectively). PMP levels were significantly higher in patients with positive anti-topoisomerase-I antibodies (p = 0.030) and in patients with a disease duration > 3 years (p = 0.038). EMP levels were lower in patients with a higher modified Rodnan skin score (p = 0.015), and in those with an avascular score > 1.5 in NFC (p = 0.042).

Conclusion
The increased levels of PMPs, EMPs and MMPs in scleroderma patients might indicate a possible role for these agents in the pathogenesis of this challenging disease.