bluuebunny/crossref_metadata_2025_split huggingface dataset:
EIF4A3-induced circ_0022382 promotes breast cancer cell progression through the let-7a-5p/PI3K/AKT/mTOR signaling pathway and SLC7A11 axis
Authors: Liu Wei , Zhang Jun , Zhang Jiawen , Ye Yu , Zhu Jianqin , Yu Qiwen , Li Tao , Sun Xiaochun , Chen Huabiao 
Published: January 2025

IntroductionBreast cancer is one of the most common cancers in women and poses a serious threat to women's health. Circular RNAs (circRNAs) have been found to be specifically expressed in cancers and regulate the growth and death of tumor cells. The role of circRNAs in breast cancer remain unknown. In this study, we explored the impacts of circRNAs on the progression of breast cancer cells.MethodsUsing bioinformatics analysis, we screened out one up-regulated circRNA in breast cancer, and its function and regulatory mechanisms were confirmed by quantitative real-time PCR, cell counting kit-8 experiment, migration assay, dual luciferase reporter assay, Kyoto Encyclopedia of Genes and Genomes enrichment analysis, cell immunofluorescence, clone formation assay, scratch wound healing experiment, RNA immunoprecipitation and subcutaneous tumor-bearing experiments.ResultsCirc_0022382 was highly expressed in breast cancer cell lines MDA-MB-231, MCF-7 as well as breast cancer tissues, and promoted the proliferative and migratory capacity of breast cancer cells. In terms of regulatory mechanisms, circ_0022382 activated PI3K/AKT/mTOR signaling pathway and SLC7A11 by sponging let-7a-5p, while knockdown of circ_0022382 contributed to the occurrence of disulfidptosis. In addition, EIF4A3 promoted the expression of circ_0022382 in MDA-MB-231 and MCF-7. Consistently, knockdown of circ_0022382 inhibited the growth of breast cancer cells in vivo.DiscussionCirc_0022382 and its related molecules may be effective targets for diagnosis or targeted therapy of breast cancer.
Efficacy and safety of the combination of anlotinib and envafolimab in the treatment of unresectable or metastatic liposarcoma: findings from a single-center retrospective study
Authors: Liu Hongliang , Hao Qisheng , Wang Xi , Cheng Mengxing , Qiu Fabo , Zhou Bin 
Published: January 2025

ObjectiveTo evaluate the efficacy and safety of anlotinib combined with envafolimab in the treatment of unresectable or metastatic liposarcoma.MethodsThis single-center, retrospective study enrolled 15 patients with unresectable or metastatic liposarcoma, who were treated at the Retroperitoneal Tumor Surgery Research Center of Qingdao University Affiliated Hospital between April 2022 and November 2023. The treatment regimen consisted of anlotinib combined with envafolimab. Treatment efficacy was evaluated using the Response Evaluation Criteria in Solid Tumors version 1.1. Treatment-related adverse events (TRAEs) were assessed using Common Terminology Criteria for Adverse Events version 5.0.ResultsA total of 15 patients with unresectable or metastatic liposarcoma were included; among them, seven were male (46.7%) and eight were female (53.3%), with a median age of 55 years. The pathological subtype distribution was as follows: three (20.0%) patients with well-differentiated liposarcoma, 11 (73.3%) patients with dedifferentiated liposarcoma, and one (6.7%) patient with myxoid liposarcoma. At 12 weeks post-diagnosis, none of the patients achieved a complete response. The objective response rate was 6.7%, with one patient (6.7%) achieving a partial response. Disease stability was observed in 10 (66.6%) patients, which corresponded to a disease control rate of 73.3%. Disease progression occurred in four (26.7%) patients. The median follow-up time was 16.9 months and the median progression-free survival time was 14.2 months. Seven patients experienced TRAEs, of whom three (42.2%) had grade 3–4 TRAEs. The most common TRAEs were liver function abnormalities, hypertension, and fatigue.ConclusionAnlotinib combined with envafolimab demonstrates promising efficacy and manageable safety in treating unresectable or metastatic liposarcoma.
Cardiovascular complications in chronic active Epstein–Barr virus disease: a case report and literature review
Authors: Feng Zhiyuan , Duan Hongyu , Wang Lin , Yu Huan , Zhou Kaiyu , Hua Yimin , Wang Chuan , Liu Xiaoliang 
Published: January 2025

BackgroundCardiovascular involvement is a rare but severe complication of Epstein–Barr virus (EBV) infections. Patients with chronic active EBV (CAEBV) are at increased risk of developing cardiovascular complications and have a poor prognosis. Here, we report the rare case of a pediatric patient with CAEBV and EBV- hemophagocytic lymphohistiocytosis (HLH) complicated with a giant coronary artery aneurysm (CAA) and thrombosis, a giant Valsalva sinus aneurysm, and ascending aorta dilation seven years after the disease onset.Case presentationA previously healthy 3-year-old girl was initially misdiagnosed as presenting incomplete Kawasaki disease complicated by coronary artery lesions (CALs) for which she received intravenous immunoglobulin and aspirin therapy. Subsequently, she was transferred to our hospital, where we diagnosed her as having a primary EBV infection. After acyclovir therapy, her clinical symptoms resolved with negative EBV-DNA, and she was discharged home with aspirin treatment for the remaining CALs. However, she did not have regular follow-ups after that. Seven years later, the 10-year-old girl developed a prolonged fever and fatigue, and she was diagnosed as presenting CAEBV and EBV-associated hemophagocytic lymphohistiocytosis (EBV-HLH) due to the presence of a high EBV-DNA load, prolonged fever, splenomegaly, bicytopenia, hypertriglyceridemia, hypofibrinogenemia, hemophagocytosis, low NK-cell activity, and increased levels of ferritin and soluble CD25. The echocardiography images showed giant left and right coronary artery aneurysms, a giant Valsalva sinus aneurysm, and ascending aorta dilation. Her parents agreed to a therapy with intravenous immunoglobulin, methylprednisolone, antiplatelet, and anticoagulant, but not to the standard therapy of EBV-HLH. However, the cardiovascular complications, including CAAs and thrombosis, Valsalva sinus aneurysm, and aorta lesions, did not resolve. Three weeks later, the patient was finally discharged home asymptomatic. Unfortunately, one month after discharge, the fever recurred the girl. The guardian had refused treatment and took the patient home due to economic difficulties. During our subsequent follow-up visit, the girl subsequently passed away.ConclusionsWe reported the case of a pediatric patient with EBV infection who developed rare and fatal cardiovascular complications (CAAs and thrombosis, Valsalva sinus aneurysm, and aortic lesions) seven years after the onset of the infection. Clinicians should be aware of these complications during the long-term follow-up of patients with EBV infection, especially in patients with CAEBV and/or EBV-HLH.
Assessment of right ventricular endocardial fibroelastosis in fetuses with critical pulmonary stenosis and pulmonary atresia with intact ventricular septum
Authors: Wang Yue , Luo Gang , Sun Yi , Chen Taotao , Pan Silin 
Published: January 2025

BackgroundThis study aimed to assess right ventricular (RV) endocardial fibroelastosis (EFE) in fetuses with critical pulmonary stenosis (CPS) and pulmonary atresia with intact ventricular septum (PA-IVS) and to investigate the implications of RV EFE for circulatory outcomes.MethodsFetal echocardiographic data from July 2018 to January 2021 were collected. Three reviewers independently graded EFE based on the presence and extent of endocardial echogenicity. Since this is a novel study on grading RV EFE, intra- and interobserver comparisons were performed. The associations among EFE severity, anatomic variables, and late-gestational circulatory outcomes were analysed.ResultsEighty-one patients with RV EFE were identified. By consensus, EFE severity was assessed as Grade 1 (n = 66, 81.5%) or Grade 2 (n = 15, 18.5%). At the first consultation, RV sphericity values were greater in Grade 2 EFE fetuses than in Grade 1 EFE fetuses, implying more severe noncompliance and worse diastolic function. From the first consultation to late gestation, significant differences were observed in the changes in the tricuspid/mitral valve (TV/MV) annulus diameter (P = 0.042) and TV z-score (P = 0.001) between the Grade 1 and Grade 2 RV EFE groups. Among the ten patients who underwent fetal cardiac intervention (FCI), the restoration of the TV z-score was more significant in Grade 2 RV EFE fetuses than in Grade 1 EFE fetuses. Among Grade 2 EFE cases, fetuses who underwent FCI exhibited greater changes in the right/left ventricular (RV/LV) long-axis dimension, TV/MV, and RV sphericity compared to non-FCI fetuses, indicating that FCI benefited Grade 2 EFE fetuses by restoring the development of ventricular structure.ConclusionsThis study graded RV EFE in fetuses with CPS/PA-IVS, shedding light on its implications for circulatory outcomes. FCI offered benefits in Grade 2 RV EFE patients, suggesting its potential to preserve cardiac development in affected fetuses with CPS/PA-IVS.
Amphotericin B tissue penetration and pharmacokinetics in healthy and Candida albicans-infected rats: insights from microdialysis and population modeling
Authors: Santos Valdeene Vieira , Pereira Laiz Campos , de Araújo Jackeline Marley Santos , Borges Matheus Antônio da Hora , Brandão Carolina Magalhães , Santos Luisa Oliveira , Villarreal Cristiane Flora , Azeredo Francine Johansson 
Published: January 2025

IntroductionThis study evaluated the relationship between total plasma and free kidney concentrations of amphotericin B (AmB) in healthy and C. albicans-infected Wistar rats using microdialysis and has the potential to significantly impact future research in this field and promote the development of antifungal drugs. The findings of this study, which show that plasma levels are a good predictor for AmB kidney concentrations and can be used to optimize its dosing regimen, underscore the importance of this research.MethodsMicrodialysis probe recovery rates were determined by dialysis and retrodialysis in vitro, as well as by retrodialysis in vivo. The intravenous (i.v.) administration of 2.5 × 106 CFU/mL of C. albicans ATCC induced the infection. A 2.5 mg/kg i.v. bolus was used in healthy and C. albicans-infected rats (n = 6/group). Plasma and microdialysate samples were analyzed using HPLC-diode-array detection. AmB tissue penetration was analyzed using the ratio between the total plasma and kidney concentrations and population pharmacokinetics (PopPK) to assess the impact of the infection on the pharmacokinetic parameters. The chosen flow rate was set to 1.5 μL/min, and there was no statistical difference between the relative recovery values when changing AmB concentrations.Results and DiscussionThe in vivo relative recovery was determined to be 10.9% ± 3.7%. The antifungal tissue penetration was 0.77 and 0.71 for the healthy and infected animals, respectively. The structural PK model with two compartments and linear elimination describes the concentration versus time profile of AmB simultaneously in the plasma and tissue. Infection by C. albicans does not interfere with AmB kidney penetration. AmB protein binding is demonstrated to be nonlinear and dependent on the AmB concentration in the plasma of healthy and infected animals.
Stellate ganglion blockade under ultrasound-guidance and the physiological responses in the rat
Authors: Tran Robert M. N. , Malik Shaista , Reist Christopher , Oh Chad K. , Abdul-Musawir Najeebah , Tjen-A-Looi Stephanie C. , Fu Liang-Wu , Baird Theodore J. , Nguyen Anh T. , Gong Yiwei D. , Guo Zhi-Ling 
Published: January 2025

Stellate ganglion blockade (SGB) is a practical approach to managing many clinical disorders. Ultrasound-guided SGB is currently adopted as a more effective and safer method in humans. Developing this technique in rats would facilitate further study of SGB application. The present study examined physiological responses following ultrasound-guided SGB in Sprague-Dawley rats. Under general anesthesia, lidocaine containing Chicago blue dye (1.0%–1.5% in 40–60 µL) was injected into the unilateral stellate ganglion (SG). Ptosis was observed on the ipsilateral right (n = 8) or left (n = 7) side of lidocaine administration. No ptosis was noted in any controls by 0.9% normal saline injection into the right (n = 6) or left (n = 6) SG. Heart rate (HR) was significantly decreased after administration of lidocaine (344 ± 32 to 289 ± 47 bpm; p = 0.015, pre-vs. after-injection), but not after normal saline, into the right SG. HR was unaltered after injecting lidocaine or normal saline into the left SG. Heart rate variability analysis showed that SGB with lidocaine on the right or left side caused a decrease in the ratio of the power of low-frequency over high-frequency. Respiratory rate, body temperature, and general conditions were unchanged in all rats, regardless of left or right SGB. Chicago blue dye was confirmed to be distributed in the SG region. No bleeding or tissue damage was evident in the injected SG area. Our findings suggest that ultrasound-guided unilateral SGB effectively inhibits cervicothoracic sympathetic nerves in rats and enhances heart rate variability, and sympathetic nerves controlling HR are likely predominantly associated with the right SG in the rat.
Short-time cycling performance in young elite cyclists: related to maximal aerobic power and not to maximal accumulated oxygen deficit
Authors: Støa Eva Maria , Rønnestad Bent , Helgerud Jan , Johansen Jan-Michael , Andersen Ingvild Tronstad , Rogneflåten Torkil , Sørensen Anders , Støren Øyvind 
Published: January 2025

PurposeTo explore the relationships between performance variables and physiological variables in a short-time (2–3 min) cycling time trial (TT) on a cycle ergometer.MethodsFifteen young elite cyclists (age: 17.3 ± 0.7 years, maximal oxygen uptake (VO2max): 76.6 ± 5.2 mL⋅kg−1⋅min−1) participated in this study. Maximal aerobic power (MAP), maximal anaerobic power (MANP), time to exhaustion at 130% of maximal aerobic power (TTE), maximal accumulated oxygen deficit (MAOD) in the TT, anaerobic power reserve (APR) and lactate threshold (LT) was tested. MAP was calculated as VO2max/oxygen cost of cycling (CC), MANP was determined as mean power output (W) during a 10 s maximal cycling sprint test, and MAOD was calculated as (VO2 demand - VO2 measured) ∙ time. APR was calculated as the relative difference between MAP and MANP.ResultsThere was a strong correlation between MAP and TT time (r = −0.91, p &lt; 0.01) with a standard error of estimate (SEE) of 4.4%, and a moderate association between MANP and TT time (r = −0.47, p = 0.04). Neither MAOD, TTE, LT nor APR correlated with TT.ConclusionMAP was highly correlated with TT with a SEE of 4.4%. Since neither TTE nor MAOD correlated with TT, this indicates that these two variables do not play a significant role in differentiating short-time endurance cycling performance. We suggest training for improving MAP and, or MANP to improve short-time endurance cycling performance.
Fruit quality evaluation of different mulberry varieties
Authors: Tian Jie , Wen Haichao , Liu Bingxiang , Tian Xinyuan , Wu Yibo , Yang Jingyan , Zhang Bingying , Li Hongjiao 
Published: January 2025

IntroductionThe quality of fruits has long been a key focus for breeders, and the development of scientifically sound and reasonable methods for evaluating fruit quality is of great significance in selecting superior cultivars. The mulberry tree, as a plant resource that serves both medicinal and dietary purposes, contains rich nutritional components and various bioactive compounds. These include properties such as immune enhancement, lipid-lowering effects, and anti-tumor activities.MethodsTherefore, to select mulberry varieties with superior quality and adapt to the diversification trends in mulberry development, this study uses 21 mulberry varieties to analyze and compare differences in fruit appearance quality, nutritional quality, functional components, and antioxidant capacity. Principal Component Analysis (PCA) was employed to identify core evaluation indices, and the Entropy Weight Method was used to assign weights based on these core quality indices. Subsequently, Grey Relational Analysis (GRA) was used for a comprehensive evaluation of the fruit quality of the 21 mulberry varieties.ResultsThe results indicate that, in terms of appearance quality, varieties such as ‘Ri Ben Guo Sang’, ‘Hong Guo 1’, ‘Lv Shen Zi’, ‘He Lan Sang’, and ‘Ju Shen’ stand out overall. In terms of nutritional quality, ‘Tang 10’ has relatively higher levels of free amino acids and soluble proteins, but its solid-acid ratio is the lowest, which affects the taste of the fruit. Overall, varieties such as ‘Jiang Mi Guo Sang’, ‘Bai Shen 2’, ‘Ji Gui Hua’, ‘Xiao Bai E’, ‘Da Bai E’, and ‘Da Yi Bai’ stand out in terms of comprehensive quality. Regarding functional components, the four varieties—’Lv Shen Zi’, ‘Hei Zhen Zhu’, ‘He Lan Sang’, and ‘Da 10’—are prominent across all indicators. In terms of antioxidant capacity, ‘Jiang Mi Guo Sang’, ‘Hong Guo 1’, ‘Xiao Bai E’, ‘Da Bai E’, and ‘Da Yi Bai’ rank relatively high, which largely overlaps with the varieties selected for their nutritional quality. Regarding fruit enzyme activity, ‘Ri Ben Guo Sang’, ‘Hong Guo 1’, ‘Lv Shen Zi 1’, ‘Lv Shen Zi 2’, ‘He Lan Sang’, and ‘Da 10’ show high enzyme activities. Finally, based on Principal Component Analysis (PCA), the fruit’s appearance quality, nutritional quality, functional components, and antioxidant capacity were categorized into seven principal components, covering 12 indicators, with a cumulative variance contribution rate of 88.424%. The Entropy Weight Method was used to assign weights to these 12 indicators, and the final correlation degree was calculated using Grey Relational Analysis (GRA), with a range from 0.406 to 0.817.ConclusionThis study suggests that varieties such as ‘Da 10’, ‘Feng Guo Sang’, ‘He Lan Sang’, ‘Lv Shen Zi’, and ‘Ri Ben Guo Sang’ exhibit superior overall fruit quality and rich nutritional value, providing a theoretical basis for the selection, development, and utilization of future mulberry fruit varieties.
Partner phubbing and relational aggression in romantic relationships among young adults in China: the roles of social support and gender
Authors: Ying Liang , Ren Lvzhou , Wang Xin , He Jiankang , Yang Xue , Zhang Guohua 
Published: January 2025

IntroductionPhubbing may have significant interpersonal consequences. This study examines the association between partner phubbing and relational aggression, the mediation effect of social support, and the moderation effect of gender among young Chinese adults.MethodA total of 772 young adults currently in a romantic relationship participated in an online survey that assessed their partner phubbing, relational aggression, and social support (i.e., ideal support, actual support, and discrepancy between ideal and actual support).ResultsThe results show that partner phubbing was positively and significantly correlated with relational aggression for males and females, respectively. Actual support and support discrepancy partially mediated the relationship between partner phubbing and relational aggression among all participants. Regarding gender difference, actual support and support discrepancy partially mediated the relationship between partner phubbing and relational aggression in females but were not significant mediators for males.DiscussionThese findings suggest that partner phubbing had a significant effect on relational aggression in romantic relationships for both male and female participants. Social support may play a significant role between partner phubbing and relational aggression in females only. Tailored interventions for partner phubbing to prevent negative interpersonal consequences are warranted.
The influence of expectations on shame, rumination and cognitive flexibility: an experimental investigation on affect-regulatory characteristics of deceptive placebos
Authors: Schäfer Leonora Nina , Rief Winfried 
Published: January 2025

BackgroundSeveral studies identified affect-regulatory qualities of deceptive placebos within negative and positive affect. However, which specific characteristics of an affect-regulatory framing impacts the placebo effect has not yet been subject to empirical investigations. In particular, it is unclear whether placebo- induced expectations of direct emotion inhibition or emotion regulation after emotion induction elicit stronger effects in affect regulation.PurposeThe aim of the study was to identify whether specifically framed expectations on the occurrence (antecedent-focused) vs. regulation capability (response-modulating) of affect, induced with an active placebo nasal-spray, have effects on affect-regulatory processes. Because personality traits have been suspected to influence placebo responses and affect regulation, an additional goal of the study was to examine modulating influences of shame proneness, level of depression, experiential avoidance, and emotional control.MethodsHealthy volunteers (n = 121) were randomized to either a deceptive placebo condition (antecedent-focused vs. response-modulating instruction) or a no-treatment control group before shame was experimentally induced via autobiographical recall. Groups were compared on outcomes of state shame, rumination, and cognitive flexibility.ResultsBoth antecedent-focused and response-modulating placebo framings influenced changes in state shame (b = 3.08, 95% CI = [0.80–5.92], p = 0.044), rumination (b = 4.80, 95% CI = [1.50–8.09], p ≤ 0.001) and cognitive flexibility outcomes (b = −3.63, 95% CI = [−6.75 – −0.51], p = 0.011) after shame-induction interventions. Only the antecedent-focused placebo response was modulated by personality traits. Experiential avoidance modulated shame experience (F(2,115) = 3.470, p = 0.031) whereas emotional control influenced the reports of state rumination (F(2,115) = 4.588, p = 0.012). No modulatory influences of levels of depression and shame proneness could be observed (ps &gt; 0.05).ConclusionThe results suggest that shame, rumination and cognitive flexibility can be positively influenced by placebo treatment in healthy subjects. Personality traits of emotional control and experiential avoidance influenced the placebo response of the antecedent-focused treatment rationale on outcomes individually.Clinical trial registrationClinicalTrials.gov, identifier NCT05372744.
Can role model women in politics help married women reduce suicides? Evidence from a quasi-experimental study in Brazil
Authors: Arvate Gabriela Gerote , Arvate Paulo , Massuda Adriano , Massuda Raffael , Atun Rifat 
Published: January 2025

BackgroundThe medical literature has demonstrated that macro-variables and social factors can influence suicide rates. Additionally, social science literature has shown that women in prominent political positions (such as mayors) can influence the behavior of other women. The purpose of our work is to demonstrate that women in such positions reduce suicide rates within a group affected by gender inequality: married women.MethodologyWe use regression discontinuity methodology and quasi-experimental electoral designs (elections with a margin of victory very close to zero) to ensure causal inference between the election of women and suicide rates among married women.Principal findingsMunicipalities that elected women as mayors have 1.33 fewer suicides among married women per 100,000 inhabitants compared to those that elected men as mayors.ConclusionThese results offer new insights into how empowered women can challenge social norms and improve public health outcomes.