bluuebunny/crossref_metadata_2025_split huggingface dataset:
Melphalan induced germ cell toxicity and dose‐dependent effects of β‐aminoisobutyric acid in experimental rat model: Role of oxidative stress, inflammation and apoptosis
Authors: Panghal Archna , Kumar Vinod , Jena Gopabandhu 
Published: April 2023

AbstractThe success of chemotherapy regimens has led to an increase in cancer survival rate over the last decades. Melphalan has been widely used for the treatment of several types of cancers despite its gonadotoxic effects. Due to its ability to cause mutations in the spermatogonial stem cells and spermatids, melphalan can exert a negative impact on male reproductive health in young cancer survivors. β‐aminoisobutyric acid (BAIBA), a myokine released by skeletal muscles, has been reported to have beneficial effects in diabetic nephropathy, cardiomyopathy and hepatic toxicity. However, the exact role of BAIBA in chemotherapy‐induced germ cell toxicity is still unexplored. The present study aims to determine the dose‐dependent (25, 50, and 100 mg/kg) effects of BAIBA on melphalan‐induced (1.5 mg/kg) germ cell toxicity in sprague−dawley (SD) rats. The evaluation parameters included quantification of oxidative stress biomarkers, sperm count, sperm motility and head morphology, sperm and testicular DNA damage, sperm mitochondrial membrane potential, ultrastructural changes in sperms, histological and protein expression studies in testes. Melphalan treatment significantly altered all the above‐mentioned parameters and the high dose (100 mg/kg) of BAIBA restored melphalan‐induced toxicity in a significant manner by exerting antioxidant, anti‐inflammatory and antiapoptotic effects. However, the medium dose (50 mg/kg) of BAIBA decreased the toxicity of melphalan and the low dose (25 mg/kg) of BAIBA failed to counteract the melphalan‐induced male germ cell toxicity as well as the peripheral blood micronucleus induction. The antioxidant, anti‐inflammatory and antiapoptotic role of BAIBA in melphalan‐induced gonadal damage is a novel finding in an experimental rat model.
The highly efficient protein crystallography beamline TLS 13B1 at the National Synchrotron Radiation Research Center
Authors: Chen Yi‐Hui , Tseng Chien‐Chang , Chou Chung‐Kuang , Liu Yi‐Chun , Chiang Cheng‐Hung , Huang Chen‐Ying , Chao Chun‐Hsiung , Huang Chun‐Hsiang 
Published: April 2023

AbstractSynchrotron protein crystallography (PX) is one of the most powerful and dominant tools for determining the three‐dimensional structures of biological macromolecules. At the National Synchrotron Radiation Research Center, TLS 13B1 is the first dedicated energy‐tunable and highly automated PX beamline for efficiently solving de novo protein structures in Taiwan and has been open for users since September 2005. With remote access, user support services, and annual PX training courses over 17 years, the number of user groups at TLS 13B1 has grown from 11 to 139. In total, 1,429 projects were supported, and 2090 experiments were hosted during 67,846 hr of beamtime. This paper describes the photon source, beamline components, experimental station equipment, beamline software, beamline application process, and results from our users.
A minimal model of potential energy surface for H2OHCl
Authors: Caglioti Concetta , Palazzetti Federico 
Published: April 2023

AbstractIn this paper, we present a model of potential energy surface for the H2OHCl system, consisting in the exact transformation of quantum chemical input data related to a minimal number of significant configurations. Both molecules are assumed as rigid. The interaction potential is given by an expansion in real spherical harmonics depending on the distance between the two centers of mass of the molecules and on four angles that define their mutual orientation. The main target of this work is the construction of a model of potential energy surface that requires a limited number of single energy points, which is suitable for applications to classical and quantum molecular dynamics simulations, permitting interpolation and further implementation of different sets of input data.
Cancer‐cell‐biomimetic nanoparticles for enhanced prostate cancer therapy
Authors: Hong Yazhao , Wang Chengming , Wang Huan , Gao Yizhou , Wu Jie , Xia Jing 
Published: April 2023

AbstractBACKGROUNDThe targeted delivery of traditional chemotherapeutic drugs by nanocarriers has become an effective way to reduce drug toxicity and improve antitumor effects. However, nanocarriers are easily recognized and cleared by the immune system after entering the body, thereby reducing their therapeutic effect.RESULTSIn this work, based on the biomimetic design concept, a tumor cell membrane‐coated nanoplatform (TCM/SCM/DTX) was fabricated for evading the clearance of the immune system and efficiently targeting tumor tissues to achieve efficient drug accumulation in tumor tissues. In vitro cell experiments, TCM/SCM/DTX could effectively enter prostate tumor cells and showed a good in vitro anti‐tumor effect. After administration, TCM/SCM/DTX could efficiently accumulate into tumor tissue while achieving long‐term blood circulation. The in vivo anti‐tumor results proved its effective tumor growth inhibitory effect, and the immunohistochemical results also proved that TCM/SCM/DTX significantly reduced the tissue and organ toxicity of DTX.CONCLUSIONSThis study shows that the prostate tumor cell membrane‐coated biomimetic nanocarriers can effectively deliver traditional chemotherapeutic drugs to prostate cancer tissue, which provides a new strategy for precise and efficient chemotherapy of prostate cancer, especially advanced prostate cancer. © 2023 Society of Chemical Industry (SCI).
Synthesis of a novel MMK/β‐CD‐A material and its tetracycline removal efficiency
Authors: Guo Chengjie , Liu Jiaoqin , Jin Riya , Qiao Yina , He Zengdi , Wang Anlong , Gao Jingshuai , Zhang Jingjing , Jia MengYe , Mao Jipeng 
Published: April 2023

AbstractBackgroundAs one of the modal antibiotics in life, the abuse of tetracycline (TC) caused great risks to the ecological environment and health. Cost‐effective removal method of TC in the medical wastewater was a developmental research direction with great potential.ResultsIn this work, a novel material, modified kaolin/β‐cyclodextrin (β‐CD) coated A (A is a special oxidant made from our laboratory, which is mainly composed of potassium ferrate) was synthesized and characterized. Meanwhile, the removal efficiencies and influencing factors of TC in novel material activation reaction systems were systematically studied. The specific surface area of modified kaolin (MMK) can reach up to 431.82 m2/g after acid and calcination modification. The results of SEM, TEM, XRD, and FT‐IR showed the significant increase in specific surface area of MMK was the complete destruction of kaolin structure and the leaching of aluminum. β‐CD was selected to coat A to attenuate the rapid release of A in water. The optimal coat molar ratio of β‐CD and A was 10: 1 (β‐CD:A). MMK/β‐CD‐A was used to treat the simulated TC wastewater, the removal efficiency was 95.6 % within 90 min when the concentration of TC was 0.22 mmol/L. The removal mechanism of TC with MMK/β‐CD‐A was the adsorption effect from MMK and the oxidation effect from β‐CD‐A.ConclusionThis work could provide useful information for developing a simple and effective technology to treat TC in medical wastewater. © 2023 Society of Chemical Industry (SCI).
Defining the influence of size‐exclusion chromatography fraction window and ultrafiltration column choice on extracellular vesicle recovery in a skeletal muscle model
Authors: Fernández‐Rhodes María , Adlou Bahman , Williams Soraya , Lees Rebecca , Peacock Ben , Aubert Dimitri , Jalal Aveen R. , Lewis Mark P. , Davies Owen G. 
Published: April 2023

AbstractExtracellular vesicles (EVs) have the potential to provide new insights into skeletal muscle (SM) physiology and pathophysiology. However, current isolation protocols often do not eliminate co‐isolated components such as lipoproteins and RNA binding proteins that could confound outcomes and hinder downstream clinical translation. In this study, we validated an EV isolation protocol that combined size‐exclusion chromatography (SEC) with ultrafiltration (UF) to increase sample throughput, scalability and purity, while providing the very first analysis of the effects of UF column choice and fraction window on EV recovery. C2C12 myotube conditioned medium was pre‐concentrated using either Amicon® Ultra 15 or Vivaspin®20 100 KDa UF columns and processed by SEC (IZON, qEV 70 nm). The resulting thirty fractions obtained were individually analysed to identify an optimal fraction window for EV recovery. The EV marker TSG101 could be detected from fractions 5 to 14, while CD9 and Annexin A2 only up to fraction 6. ApoA1+ lipoprotein co‐isolates were detected from fraction 6 onwards for both protocols. Strikingly, Amicon and Vivaspin UF concentration protocols led to qualitative and quantitative variations in EV marker profiles and purity. Eliminating lipoprotein co‐isolation by reducing the SEC fraction window resulted in a net loss of particles, but increased measures of sample purity and had only a negligible impact on the presence of EV marker proteins. In conclusion, our study developed an effective UF+SEC protocol for the isolation of EVs based on sample purity (fractions 1–5) and total EV abundance (fractions 2–10). We provide evidence to demonstrate that the choice of UF column can affect the composition of the resulting EV preparation and needs to be considered when being applied in EV isolation studies in SM. The resulting protocols will be valuable in isolating highly pure EV preparations for applications in a range of therapeutic and diagnostic studies.
Ecomorphological variation of the penguin wing
Authors: Haidr Nadia Soledad 
Published: April 2023

AbstractPenguins (Aves, Sphenisciformes) are pursuit divers that feed mainly on krill, fish, and squid. Although they are opportunistic feeders, some species are more generalists than others and many show dietary preferences toward krill and other crustaceans or fish and squid. Their diving depth seems to follow a body size pattern and relates to the type of item that they prey on. Penguins dive with their wing; hence their wing musculature is responsible for the animal maneuverability and strength while diving. In the present study, ecological traits such as diving depths and prey composition are used to explore if morphology relates to foraging habits. A geometric morphometric approach is used to quantitatively address these morphological differences in the wing apparatus of all extant penguins and a fossil species taking into consideration allometric and phylogenetic factors. Results show that morphological differences among penguins with different diets are significant and strong; groups are well separated with the greatest differences found between piscivorous and crustacivorous penguins. Dive depth has a moderate covariation with morphology and a strong correspondence with wing area. Last,Madrynornis mirandus, an exceptionally well‐preserved fossil from the Miocene of Patagonia, is found to be close to the piscivorous and generalist piscivorous species. It is proposed that swimming styles correlate with specific traits of the anatomy of wing and pectoral girdle skeleton and muscles.
Regulatory B cells induced by interleukin‐35 inhibit inflammation and alveolar bone resorption in ligature‐induced periodontitis
Authors: Li Shiyi , Su Li , Luan Qingxian , Liu Guojing , Zeng Wenmin , Yu Xiaoqian 
Published: April 2023

AbstractBackgroundRegulatory B cells (Bregs) have been reported to suppress immune responses and alveolar bone loss in murine periodontitis models. These cells could be induced by interleukin (IL)‐35 which is increased upon periodontal inflammation. Thus, this study aimed to explore the role of Bregs induced by IL‐35 in periodontitis.MethodsExperimental periodontitis was induced in mice by ligature. Two weeks after ligation, the test group was systemically treated with IL‐35 for 1 week. Four weeks after ligation, all mice were euthanized, and alveolar bone loss was evaluated by microcomputed tomography. Cytokines associated with periodontitis were analyzed using reverse transcription‐quantitative polymerase chain reaction and enzyme‐linked immunosorbent assay. Bregs in spleens, cervical lymph nodes, and periodontal tissues were detected by flow cytometry and immunofluorescence staining.ResultsIn the mouse model of periodontitis, IL‐35 induced the expansion of CD1dhiCD5+ B10 cells with increased interleukin‐10 (IL‐10) and IL‐35 production. IL‐35 administration also attenuated alveolar bone loss and reduced the levels of proinflammatory cytokines in situ.ConclusionsFollowing ligature‐induced periodontitis in mice, IL‐35 inhibited periodontal inflammation and alveolar bone resorption at least partially through the induction of B10 cells and IL‐35+ Bregs.
Hyperspectral imaging for the determination of relevant cooking quality traits of boiled cassava
Authors: Meghar Karima , Tran Thierry , Delgado Luis Fernando , Ospina Maria Alejandra , Moreno Jhon Larry , Luna Jorge , Londoño Luis , Dufour Dominique , Davrieux Fabrice 
Published: April 2023

AbstractBACKGROUNDThe purpose of this study was to investigate the potential of hyperspectral imaging for the characterization of cooking quality parameters, dry matter content (DMC), water absorption (WAB), and texture in cassava genotypes contrasting for their cooking quality.RESULTSHyperspectral images were acquired on cooked and fresh intact longitudinal and transversal slices from 31 cassava genotypes harvested in March 2022 in Colombia. Different chemometric methods were tested for the quantification of DMC, WAB, and texture parameters. Data analysis was conducted through partial least squares regression, K nearest neighbors regression, support vector machine regression and CovSel multiple linear regression (CovSel_MLR). Efficient performances were obtained for DMC using CovSel_MLR with, coefficient of multiple determination , root‐mean‐square error of prediction RMSEP = 0.96 g/100 g, and ratio of the standard deviation values RPD = 3.60. High heterogeneity was observed between contrasting genotypes. The predicted distribution of DMC within the root can be homogeneous or heterogeneous depending on the genotype. Weak predictions were obtained for WAB and texture parameters.CONCLUSIONSThis study showed that hyperspectral imaging could be used as a high‐throughput phenotyping tool for the visualization of DMC in contrasting cooking quality genotypes. Further improvement of protocols and larger datasets are required for WAB and texture quality traits. © 2023 The Authors. Journal of The Science of Food and Agriculture published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.
High‐performance, coplanar amorphous InGaZnO thin‐film transistors by spray pyrolysis on polyimide substrate for low‐cost manufacturing of foldable active‐matrix organic light emitting diode display
Authors: Bae Jinbaek , Ali Arqum , Park Chanju , Jang Jin 
Published: April 2023

AbstractWe report a method for low‐cost deposition of bubble‐free, high‐quality amorphous InGaZnO (a‐IGZO) on polyimide (PI) substrate for foldable active‐matrix organic light emitting diode (AMOLED) display by spray pyrolysis. The coplanar thin‐film transistor (TFT) with spray‐pyrolyzed (SP) a‐IGZO on PI substrate exhibits threshold voltage (VTH) of −0.8 V, saturation mobility of 40.95 cm2 V−1 s−1, and subthreshold swing of 0.18 V per decade with on/off drain current ratio of ~108. The high mobility TFT could be achieved using high substrate temperature (350°C) and low contact resistance by NF3 plasma on the SP a‐IGZO. The TFT shows a ΔVTH of −0.4 V when it is bent on a cylinder of 1‐mm radius. The TFT shows the ΔVTH of +0.05 V for positive bias temperature stress at +20 V at 60°C for 1 h. The ring oscillator made of a‐IGZO TFTs exhibits an oscillation frequency of 2.38 MHz at VDD of 10 V with a propagation delay of 9.13 ns per stage. Therefore, the a‐IGZO TFT by spray pyrolysis could be used for low‐cost, high‐performance, and flexible display TFT backplane.
Analysis of oasis land ecological security and influencing factors in arid areas
Authors: Wang Lu , Chang Jianxia , He Bing , Guo Aijun , Wang Yimin 
Published: April 2023

AbstractArid oasis areas are typical ecologically fragile areas, and Land ecological security (LES) reflects the relationship between oasis land use and socio‐economics. At present, the evaluation of oasis LES is still in the exploration stage, and there is a lack of dynamic evaluation of LES from a spatial and temporal perspective, and the interaction between influencing factors is not considered, which often affects the reliability of LES evaluation results and the optimization of land scenarios. This study analyzes the dynamic changes in land use in the Tarim River Basin (TRB) oasis from 1990 to 2020 and constructs a pressure‐state‐response (PSR) model to assess the spatial and temporal patterns of the LES. Then, the spatial correlation and spatial divergence of the LES were explored using the Moran index. Finally, the geographical detector analysis was used to reveal the drivers of the spatiotemporal divergence of LES patterns in the TRB oasis and the interaction effects of the influencing factors. The results reveal that there is significant spatial and temporal variability in the LES level of TRB oases, with the eastern region continuing to deteriorate while the western region continues to improve. Between 1990 and 2020, the largest increase in TRB oases was 24,089.52 km2 of cultivated land and the largest decrease was 47,117.03 km2 of grassland. Also, there are interactions between the variables. The greatest associations between the three variables‐the forest coverage raste, the proportion of the tertiary industry, and rural per capita net income‐all reached over 0.88. This study evaluates the LES and analyzes the affecting factors, which contribute to the development of refined land optimization countermeasures and offers a foundation for the realization of sustainable land use in oases and the establishment of oasis ecological civilization.
The efficacy of two commercially available devices for airway foreign body relief: A cadaver study
Authors: Ramaswamy Apoorva , Done Aaron , Solis Roberto , Srikanth Mayuri , Olinde Lindsay , Belafsky Peter 
Published: April 2023

AbstractObjectiveForeign body aspiration events are frequent in young children and in the geriatric population. They may result in several complications such as hypoxia, edema, cardiac arrest, and death. Recently, two commercially available devices, the LifeVac and DeChoker, have entered the market with the claim of relieving foreign body aspiration. Both devices are portable, nonpowered, suction devices that are being considered for use in large public spaces such as schools, airports, and malls despite previous studies detailing variable efficacy. In this study, we aim to contribute further data on the safety and efficacy of these devices through a fresh cadaver model.MethodsCommonly aspirated foods of three different sizes (saltines, grapes, and cashews) were placed at the level of the true vocal folds in a fresh cadaver. Three participants performed two trials with each food and device. Device use was performed to manufacturer specifications.ResultsThe DeChoker resulted in gross injury to the tongue and failed to remove the obstruction in all trials. LifeVac was successful in removing the barium‐moistened saltines but failed to remove all other foreign bodies. Both devices applied significant pressure to the tongue.ConclusionWith the exception of the LifeVac removing saltine crackers, all trials were entirely unsuccessful in relieving foreign body aspiration. Additionally, both devices may cause significant pressure and injury to the oral cavity in a clinical setting. We conclude bystanders should continue to follow International Liaison Committee on Resuscitation's guidelines on resuscitation to aid with relieving foreign body aspiration.Level of Evidence4
Dural sinus narrowing in patients with spontaneous anterior skull base cerebrospinal fluid leak
Authors: Asi Karim W. , Cameron Brian H. , Friedman Elliot R. , Radabaugh Jeffrey P. , Citardi Martin J. , Luong Amber U. , Yao William C. 
Published: April 2023

AbstractObjectivesCurrent evidence suggests a link between idiopathic intracranial hypertension (IIH) and spontaneous cerebrospinal fluid (sCSF) leak, as well as between IIH and dural venous sinus (DVS) narrowing. However, there are limited data linking DVS narrowing and sCSF leak. This study aims to determine the prevalence of DVS narrowing in patients with sCSF leak.MethodsA retrospective review of all patients with sCSF leak that presented to a tertiary academic center from 2008 to 2019. Preoperative imaging was independently reviewed by two neuroradiologists to evaluate for DVS narrowing. Available literature was used to estimate the prevalence of DVS narrowing in the general population to allow for comparison. Data were analyzed using Exact binomial test.ResultsAnalysis of 25 patients with appropriate imaging revealed the majority were women (21/25, 84%) with a mean age of 51.89 years (SD 13.96). The majority of these patients were found to have narrowing of the DVS (20/25, 80%). In patient with sCSF leaks, there was a significantly higher proportion of patients with DVS narrowing compared with published literature examining this condition in the general population (80% vs. 40%, CI 0.59–0.93, p < .001).ConclusionThe prevalence of DVS narrowing in patients with sCSF leaks is substantial and likely greater than the general population. Moreover, there appears to be narrowing in most patients with sCSF leak. Preoperative radiological evaluation of the DVS using MR venography may be useful in patients with sCSF leaks as DVS stenosis may be an underdiagnosed etiology. Further study is needed to evaluate this.Level of EvidenceIV.
Preparation and Enhancing Properties of pH‐Sensitive Hydrogel in Light of Gum Ghatti‐cl‐poly(acrylic acid)/ – o‐MWCNT for Sodium Diclofenac Drug Release
Authors: Dave Pragnesh N. , Macwan Pradip M. , Kamaliya Bhagvan 
Published: April 2023

AbstractGum ghatti (GG) is used as a biopolymer, acrylic acid (AA) is used as a synthetic monomer, ammonium persulphate (APS) is used as an initiator, and methylene bis‐acrylamide (MBA) is used as a cross‐linker in the current study to create gum ghatti‐cl‐poly(acrylic acid)‐o‐MWCNT hydrogels. The –o‐MWCNT (0, 10, 20, 30, 40, and 50 mg) is added to the hydrogel as a filler. Fourier transform infrared (FTIR), differential scanning calorimetry (DSC), scanning electron microscopy (SEM), and X‐ray diffraction (XRD) analyses are used to characterize the crosslinked hydrogels. The successful polymerization of the graft is confirmed by these spectroscopic studies. Sodium diclofenac (SD) as a model drug is utilized. Swelling tests are also conducted at pH 6.8 on all prepared hydrogels. In a similar manner, all hydrogel preparations are subjected to an in vitro study at neutral (pH 7.4), acidic (pH 1.2), and basic (pH 9.2) mediums, and a higher drug release is observed at pH 7.4. The Higuchi model, the Korsmeyer–Peppas model, and various zero‐order and first‐order kinetics are utilized in the investigation of the drug release order mechanism from the hydrogels. The drug release data favor the Korsmeyer–Peppas model, which describes the “n” diffusion exponent that controls the drug release mechanism from synthesized hydrogels. The “n” values control the Fickian diffusion (Case‐I diffusional) like coupled diffusion (0.45 ≤ n). According to the results presented here, GGAACNT‐based hydrogels can be used in biomedical fields, especially for controlled drug release.