bluuebunny/crossref_metadata_2025_split huggingface dataset:
Evaluation of a patient‐specific 3D‐printed guide for ventral slot surgery in dogs: An ex vivo study
Authors: Walker Meagan A. , Ogilvie Adam T. , McSorley Grant , Montelpare William , Hoddinott Katie L. 
Published: January 2025

AbstractObjectiveTo evaluate the accuracy of ventral slot creation in canine cadavers with a three‐dimensional (3D)‐printed drill guide compared to the freehand technique.Study designEx vivo study.Sample populationEight canine cadavers (23.4–39.8 kg).MethodsComputed tomography (CT) data was used to create patient‐specific 3D‐printed surgical guides for ventral slot creation. Intervertebral sites were randomized to undergo either a guided (n = 12) or freehand (n = 12) ventral slot by a novice surgery resident. Postoperative CT images were used to compare ventral slot dimensions, shape, and position.ResultsFree‐hand ventral slots were significantly shorter than the intended dimensions (p < .01). Dimensions of the guide‐assisted ventral slots were not statistically different from the planned dimensions (p = .88, p = .72). Use of the guides resulted in improved accuracy for ventral slot positioning relative to midline and slot shape (difference in coefficient of variations, 32%, and 40%, respectively).ConclusionVentral slot dimensions were more accurate when created with the patient‐specific 3D‐printed guide compared to the freehand technique.Clinical significanceUse of a 3D‐printed patient specific surgical guide improves accuracy of ventral slot creation in canine cadavers and improves surgical precision when used by a single novice surgical resident. The results of this study support evaluation of the guides in small breed cadavers and live patients.
Hydro, Wave and Tidal Energy Applications
Authors: Rao K.R 
Published: January 2025

The fourth installment in ASME's Renewable Energy Series, Hydro, Wave and Tidal Energy Applications offers a comprehensive update to global energy resources previously discussed in the Energy and Power Generation Handbook, 2011. Building upon the foundation of its predecessors on Solar, Biomass and Waste, and Wind Energy, this volume delivers essential insights into hydro, wave, and tidal energy advancements worldwide.
Drawing from extensive secondary sources and global perspectives, this volume provides engineers, policymakers, and renewable energy researchers with a vital resource for understanding the evolving role of hydro, wave, and tidal energy in today's energy landscape. A must-read for professionals dedicated to advancing sustainable energy solutions across the globe.
Global Wave and Tidal Energy
Authors: Rao K. R. 
Published: January 2025

Marine Energy is not necessarily same as Ocean Power. Marine energy can be created in the ocean as also in rivers, lakes, streams, estuaries, and more. The difference between ocean energy and tidal energy is that tidal energy uses gravitational pull of earth, moon and sun to produce energy whereas wave energy uses kinetic forces of waves to generate electricity. The tides at shorelines of oceans will rise and fall twice a day.
Marine energy or marine power is also referred to as ocean energy, ocean power, or marine and hydrokinetic energy. energy refer to the energy carried by ocean waves, tides salinity, and ocean temperature differences. The movement of water in the world’s oceans creates a vast store of kinetic energy, or energy in motion. Some of this energy can be harnessed to generate electricity to power homes, transport and industries. The term marine energy encompasses both wave power i.e., power from surface waves, and tidal power obtained from the kinetic energy of large bodies of moving water. The oceans have a tremendous amount of energy and are close to many if not most concentrated populations. Ocean energy has the potential of providing a substantial amount of new renewable energy around the world. substantial amount of renewable energy around the world.
Evaluating small extracellular vesicle-based vaccination across heterologous
Salmonella
strains isolated from wastewater
Authors: Emerson Lisa E. , Bhimani Saloni , Rainey Andrew L. , Maurelli Anthony T. , Ferraro Mariola J. 
Published: January 2025

ABSTRACT

Salmonella
infections pose significant public health challenges worldwide. The diversity of
Salmonella
strains, particularly those isolated from environmental and clinical sources, necessitates innovative approaches to prevention and treatment. Previous research has shown that small extracellular vesicles (sEVs) produced by macrophages during
Salmonella
Typhimurium infection can induce robust immune responses when used as a vaccine, offering complete protection in systemic infection models. In this study, we isolated 120
Salmonella
strains from qPCR
invA
-positive wastewater samples collected in Gainesville, FL. These strains underwent enrichment, selection, and biochemical confirmation, followed by serotyping and whole genome sequencing. Two isolates,
Salmonella enterica
subsp. diarizonae (Diarizonae) and
S. enterica
serovar Enteritidis, were selected for further analysis based on community prevalence and clinical severity. We also assessed the ability of sEVs produced by
S
. Typhimurium-infected macrophages to induce immune responses against these heterologous and circulating strains in mice. Immunization with sEVs induced robust antigen-specific SIgA and IgG responses against
S
. Typhimurium, Enteritidis, and Diarizonae, with high titers observed in sera and fecal samples. Proteomic analysis revealed differential expression of proteins in these strains, including antigenic proteins present in sEVs such as OmpA, FliC, or OmpD. Moreover, this study highlights the role of wastewater-based epidemiology (WBE) as a tool for environmental surveillance, offering a complementary perspective on
Salmonella
dynamics within a population. Integrating WBE with traditional surveillance methods, along with the promising results of sEV-based vaccination, provides a pragmatic strategy for developing effective preventative measures against
Salmonella
infections, addressing the diversity of non-typhoidal
Salmonella
strains.
Single-molecule localisation microscopy (SMLM) is feasible in human and animal formalin fixed paraffin embedded (FFPE) tissues in medical renal disease
Authors: Brockmoeller Scarlet F , Slaney Hayley , Curd Alistair , Bono Aurora , Felce James H , Arora Deep , Lewington Andrew , Miklosi Andras G , Quirke Philip 
Published: January 2025

AimsEstablishment of a protocol for routine single-molecule localisation microscopy (SMLM) imaging on formalin fixed paraffin embedded (FFPE) tissue using medical renal disease including minimal change disease (MCD) and focal segmental glomerulosclerosis (FSGS).MethodsProtocol for normal and diseased renal FFPE tissue was developed to investigate the clinical diagnostic potential of SMLM. Antibody concentrations were determined for confocal microscopy and transferred to SMLM. Different fixatives and lengths of fixation were studied. To reduce autofluorescence, additional quenching and UV bleaching steps were compared. Optimal SMLM acquisition settings were established. SMLM data were imaged, digitally captured, stored, visually inspected and analysed quantitatively.ResultsProtocol was established on normal renal FFPE tissue and then applied to clinical diseased tissue with single and multiple markers. Antibodies against key diagnostic proteins including podocin, nephrin, collagen, laminin, synaptopodin, CD31, IgG, IgM and IgA antibodies were established for MCD, FSGS and immune-mediated renal disease. We found important characteristic differences in the renal diseases listed above.ConclusionsWe established a routine super-resolution microscopy protocol for clinical FFPE material on medical renal biopsies, which could visualise fluorescently labelled proteins in all glomeruli present with a precision of approximately 10–20 nm, with a turnaround under 48 hours. We visualised and quantitated specific protein distributions in different conditions. SMLM opens subcellular microscopy in FFPE to histopathologists on routine FFPE tissue, which can in the future be an adjunct and, in some aspects, a rapid superior alternative to electron microscopy.
Intelligent Identification of Security Threats in Long-Distance Communication Networks Based on Embedded AI Perception
Authors: Liu Hong-Xia , Song Lina 
Published: January 2025

In the context of network security, when threats exhibit spatial similarities — such as attacks originating from the same or nearby geographic locations or using similar methods within a specific region — it can complicate the identification of security threats in long-distance communication networks. To address this challenge and improve both the efficiency and accuracy of threat identification, we propose an intelligent method for detecting long-distance communication network security threats based on embedded AI perception. This method takes spatial similarities into account by comparing sensing data between nodes within the network to assess their real-time status. Using a group decision-making mechanism, we correlate the data collection points of vulnerable network nodes to gather security log data. The network logs are then preprocessed using the Grubbs statistical algorithm to extract key security threat features. By incorporating machine learning algorithms from the field of AI, and leveraging the edge computing capabilities of the NVIDIA Jetson series as the embedded AI platform, we construct an intelligent perception model to detect security threats in real-time. Experimental results demonstrate that the proposed method can effectively detect the actual state of different nodes within the network, with a maximum state deviation of only 0.2. Additionally, the method achieved an [Formula: see text]1 score of 0.986, a recall value of 0.979, and a precision value of 0.981, indicating strong robustness and comprehensive threat identification performance.
Typhoid Fever as a Cause of Liver Failure in the United States: A Case Report
Authors: Baqir Syed Mujtaba , Sharma Neha , Lutaf Aruge , Ghitan Monica , Lin Yu Shia 
Published: January 2025

Background: Typhoid fever is a multisystemic illness caused by Salmonella typhi and Salmonella paratyphi, transmitted fecal orally through contaminated water and food. It is a rare diagnosis in the US, with most cases reported in returning travelers. Hepatitis and cholestasis are rare sequelae of salmonella infection. However, acute liver failure (ALF) is exceptionally uncommon. We report a case of typhoid fever in a returning traveler to the US progressing to ALF.Case Presentation: A 48‐year‐old man presented with high‐grade fever, abdominal pain, vomiting, acholic stools, dark urine, and yellowish discoloration of skin and sclera for one week. He was immune to hepatitis A and B, with no recent change in medications. He had no history of alcohol consumption. On presentation, the patient was tachycardic but well perfused with diffuse abdominal tenderness. Laboratory results showed leukocytosis, elevated creatinine, mixed hepatocellular and cholestatic pattern of raised liver enzymes, elevated ammonia levels, and negative hemolytic parameters. Viral, autoimmune, and metabolic causes of hepatitis were negative. Ultrasound of the abdomen revealed a normal biliary system and a computerized tomography (CT) scan of the abdomen showed multiple liver cysts, mesenteric and porta‐hepatis lymphadenopathy, and mild thickening of the terminal ileum. Intravenous (IV) ceftriaxone and metronidazole were initiated. Blood cultures grew S. typhi. The patient clinically deteriorated and developed altered mental status, respiratory distress, and an up‐trending Model for End‐Stage Liver Disease (MELD) score and was upgraded to the intensive care unit. IV meropenem was initiated, resulting in clinical recovery and negative repeat blood cultures. The patient completed 2 weeks of meropenem and was discharged.Conclusion: Typhoid fever can cause life‐threatening liver failure which is rare. Clinicians should be aware of this due to the rapid progression and life‐threatening clinical course, as well as the rise of multidrug‐resistant and extensively drug‐resistant typhoid causing delays in starting the right antibiotic.
Anterior Uveitis and Uveal Depigmentation in a Dog With Vitiligo
Authors: Ledbetter Eric C. , Lamkin Irini D. , Peters-Kennedy Jeanine 
Published: January 2025

Objective: The objective of this study is to describe the clinical and histologic features of a dog that developed anterior uveitis and uveal depigmentation in association with vitiligo.Animal Studied: A 3‐year‐old, female‐spayed, Bernese Mountain Dog with a history of bilateral idiopathic anterior uveitis developed iris depigmentation, leukotrichia, and skin depigmentation.Procedures: The initial diagnostic evaluation for uveitis was unremarkable, including general bloodwork, urinalysis, infectious disease testing, thoracic radiographs, and abdominal ultrasound. After the development of dermatologic disease, uveodermatologic syndrome was clinically suspected and cutaneous biopsy specimens were collected for histopathology.Results: Cutaneous histopathology was consistent with vitiligo. Progressive and diffuse skin and hair depigmentation occurred over several years, but the dog’s anterior uveitis remained well controlled on relatively minimal topical anti‐inflammatory medications. No posterior segment ocular lesions developed, and the dog remained visual.Conclusions and Clinical Relevance: This report indicates that anterior uveitis and uveal depigmentation can develop in dogs associated with vitiligo. The presence of bilateral uveitis and uveal depigmentation, concurrent with skin and hair depigmentation, is often considered suggestive of uveodermatologic syndrome in a dog. This report illustrates the importance of cutaneous histopathology to confirm a clinical suspicion even in the most suggestive of clinical presentations.
ZNFX1 functions as a master regulator of epigenetically induced pathogen mimicry and inflammasome signaling in cancer
Authors: Stojanovic Lora , Abbotts Rachel , Tripathi Kaushelendra , Coon Collin M. , Rajendran Saranya , Abbasi Farid Elnaz , Hostetter Galen , Guarnieri Joseph W. , Wallace Douglas C. , Liu Sheng , Wan Jun , Calendo Gennaro , Marker Rebecca , Gohari Zahra , Inayatullah Mohammed M.A. , Tiwari Vijay K. , Kader Tanjina , Santagata Sandro , Drapkin Ronny , Kommoss Stefan , Pfisterer Jacobus , Konecny Gottfried E. , Coopergard Ryan , Issa Jean-Pierre J. , Winterhoff Boris J.N. , Topper Michael J. , Sandusky George E. , Miller Kathy D. , Baylin Stephen B. , Nephew Kenneth P. , Rassool Feyruz V. 
Published: January 2025

Abstract
DNA methyltransferase and poly (ADP-ribose) polymerase inhibitors (DNMTis, PARPis) induce a stimulator of interferon genes (STING)-dependent pathogen mimicry response (PMR) in ovarian and other cancers. Here, we showed that combining DNMTis and PARPis upregulates expression of the nucleic-acid sensor NFX1-type zinc finger-containing 1 protein (ZNFX1). ZNFX1 mediated induction of PMR in mitochondria, serving as a gateway for STING-dependent interferon/inflammasome signaling. Loss of ZNFX1 in ovarian cancer cells promoted proliferation and spheroid formation in vitro and tumor growth in vivo. In patient ovarian cancer databases, expression of ZNFX1 was elevated in advanced stage disease, and ZNFX1 expression alone significantly correlated with an increase in overall survival in a phase 3 trial for therapy-resistant ovarian cancer patients receiving bevacizumab in combination with chemotherapy. RNA-sequencing revealed an association between inflammasome signaling through ZNFX1 and abnormal vasculogenesis. Together, this study identified that ZNFX1 as a tumor suppressor that controls PMR signaling through mitochondria and may serve as a biomarker to facilitate personalized therapy in ovarian cancer patients.
Taking a Swing at TIMP1-Armed Immunosuppressive Astrocytes Unleashes T cell Immunity against Brain Metastases
Authors: Lorger Mihaela , James Fiona 
Published: January 2025

Summary:
Priego and colleagues identify a secreted glycoprotein TIMP1, expressed downstream of the transcription factor STAT3, in a subpopulation of STAT3+ reactive astrocytes as a mediator of immunosuppression in late-stage brain metastases. The STAT3 inhibitor silibinin enhances the preclinical efficacy of the combined PD-1/CTLA4 immune checkpoint blockade, providing a rationale to translate the combination therapy into clinical use for this underserved patient group with poor prognosis.
See related article by Priego et al., p. 179
SWOT Data Assimilation with Correlated Error Reduction: Fitting Model and Error Together
Authors: Gille Sarah T. , Gao Yu , Cornuelle Bruce D. , Mazloff Matthew R. 
Published: January 2025

Abstract
The Surface Water Ocean Topography (SWOT) satellite mission provides high-resolution two-dimensional sea surface height (SSH) data with swath coverage. However, spatially correlated errors affect these SSH measurements, particularly in the cross-track direction. The scales of errors can be similar to the scales of ocean features. Conventionally, instrumental errors and ocean signals have been solved for independently in two stages. Here, we have developed a one-stage procedure that solves for the correlated error at the same time that data are assimilated into a dynamical ocean model. This uses the ocean dynamics to distinguish ocean signals from observation errors. We test its performance relative to the two-stage method using simplified dynamics and a data set consisting of westward propagating Rossby waves, along with correlated instrumental errors of varying magnitudes. In a series of tests, we found that the one-stage approach consistently outperforms the two-stage approach when estimating SSH signal and correlated errors. The one-stage approach can recover over 95% of the SSH signal, while skill for the two-stage approach drops significantly as error increases. Our findings suggest that solving for the correlated errors within the assimilation framework can provide an effective analysis approach, reducing the risks of confounding signal and instrument noise.
Development and Validation of a Postprocedure Stroke Screening Tool in Children With Cardiac Disease
Authors: Harrar Dana B. , Salussolia Catherine L. , Keith Louisa G. , Ye Shangyuan , Meadows Julie S. , Fialkow Alexandra , Zhu Guangyu , VanderPluym Christina , Zhang Bo , Rivkin Michael J. 
Published: January 2025

Background Cardiac catheterization in children with heart disease is associated with an increased risk of arterial ischemic stroke. We created and evaluated the diagnostic performance of a bedside screening tool administered postprocedure to identify arterial ischemic stroke. Methods We developed a postprocedure stroke screen comprising history of stroke, responsiveness, command following, speech, facial and limb strength symmetry, new seizure, and caregiver concern. We compared the performance of the screening tool in a retrospective cohort of 21 patients with a postcatheterization arterial ischemic stroke and a prospective cohort of 100 consecutive control patients after cardiac catheterization. Results The postprocedure stroke screen delivered a maximum Youden index ranging from 0.810 to 0.937 at threshold scores of 2 or 2.5 and gave a sensitivity and specificity >0.9. The area under the receiver operating characteristic curve ranged from 0.931 to 0.946. Conclusions We developed a screening tool with high sensitivity and specificity to identify postcatheterization arterial ischemic stroke in children.
A multimodal similarity-aware and knowledge-driven pre-training approach for reliable pneumoconiosis diagnosis
Authors: Ren Xueting , Ji Guohua , Chu Surong , Yoshida Shinichi , Zhao Juanjuan , Jia Baoping , Qiang Yan 
Published: January 2025

Background Pneumoconiosis staging is challenging due to the low clarity of X-ray images and the small, diffuse nature of the lesions. Additionally, the scarcity of annotated data makes it difficult to develop accurate staging models. Although clinical text reports provide valuable contextual information, existing works primarily focus on designing multimodal image-text contrastive learning tasks, neglecting the high similarity of pneumoconiosis imaging representations. This results in inadequate extraction of fine-grained multimodal information and underutilization of domain knowledge, limiting their application in medical tasks. Objective The study aims to address the limitations of current multimodal methods by proposing a new approach that improves the precision of pneumoconiosis diagnosis and staging through enhanced fine-grained learning and better utilization of domain knowledge. Methods The proposed Multimodal Similarity-aware and Knowledge-driven Pre-Training (MSK-PT) approach involves two stages. In the first stage, we deeply analyze the similar features of pneumoconiosis images and use a similarity-aware modality alignment strategy to explore the fine-grained representations and associated disturbances of pneumoconiosis lesions between images and texts, guiding the model to match more appropriate feature representations. In the second stage, we utilize data-associated features and pre-stored domain knowledge features as priors and constraints to guide the downstream model in the visual domain without annotations. To address potential erroneous labels generated by model predictions, we further introduce an uncertainty threshold strategy to mitigate the negative impact of imperfect prediction labels and enhance model interpretability. Results We collected and created the pneumoconiosis chest X-ray (PneumoCXR) dataset to evaluate our proposed MSK-PT method. The experimental results show that our method achieved a classification accuracy of 81.73%, outperforming the state-of-the-art algorithms by 2.53%. Conclusions MSK-PT showed diagnostic performance that matches or exceeds the average radiologist's level, even with limited labeled data, highlighting the method's effectiveness and robustness.
Comparison of Functional Electrical Stimulation Cycle vs. Conservative Rehabilitation on Functional Status and Muscle Features in Stroke Patients
Authors: İbişoğlu Zeynep Şafak , Kilinc Serdar 
Published: January 2025

Background Functional Electrical Stimulation (FES) Cycle is used in the rehabilitation of stroke. Objective The aim of this study is to investigate the effects of CR and FES cycle therapy on the functional status and muscle features in stroke patients Methods The 1st Group(n = 15) received only CR for 6 weeks, and the 2nd Group(n = 15) received FES cycle 3 days a week for 4 weeks in addition to the CR treatment. Brunnstrom recovery staging(BRS), Fugl-Meyer lower extremity evaluation(FME); Berg Balance Scale(BBS), Functional Ambulation Classification(FAC), Barthel Index(BI), Fatigue Severity Scale(FSS), 6 min walk test(6mWT), knee extensor and knee flexor muscle strengths(KEMS, KFMS) in both groups were assessed before and after treatment(6th week). Pennate angle(PA) and rectus femoris muscle thickness(RFT) were measured. Results Significant improvement was detected in BBS, FAS, BI, FME, 6mWT, KEMS, RFT scores after intra-group treatment compared to before treatment in both groups. Only the 2nd Group showed a significant difference in KFMS, FSS, BRS lower extremity, PA values after treatment compared to before. No significant superiority of the groups over each other was detected. Conclusions CR and FES Cycle contribute to the improvement of the functional status of the patients, but their superiority over each other was not observed.