bluuebunny/crossref_metadata_2025_split huggingface dataset:
Scheffersomyces stipitis ability to valorize different residual biomasses for vitamin B9 production
Authors: Mastella Luca , Senatore Vittorio , Beltrani Tiziana , Branduardi Paola 
Published: December 2022

AbstractSugar beet pulp (SBP), sugar beet molasses (SBM) and unfermented grape marcs (UGM) represent important waste in the agro‐food sector. If suitably pre‐treated, hexose and pentose sugars can be released in high quantities and can subsequently be used by appropriate cell factories as growth media and for the production of (complex) biomolecules, accomplishing the growing demand for products obtained from sustainable resources. One example is vitamin B9 or folate, a B‐complex vitamin currently produced by chemical synthesis, almost exclusively in the oxidized form of folic acid (FA). It is therefore desirable to develop novel competitive strategies for replacing its current fossil‐based production with a sustainable bio‐based process. In this study, we assessed the production of natural folate by the yeast Scheffersomyces stipitis, investigating SBM, SBP and UGM as potential growth media. Pre‐treatment of SBM and SBP had previously been optimized in our laboratory; thus, here we focused only on UGM pre‐treatment and hydrolysis strategies for the release of fermentable sugars. Then, we optimized the growth of S. stipitis on the three media formulated from those biomasses, working on inoculum pre‐adaptation, oxygen availability and supplementation of necessary nutrients to support the microorganism. Folate production, measured with a microbiological assay, reached 188.2 ± 24.86 μg/L on SBM, 130.6 ± 1.34 μg/L on SBP and 101.9 ± 6.62 μg/L on UGM. Here, we demonstrate the flexibility of S. stipitis in utilizing different residual biomasses as growth media. Moreover, we assessed the production of folate from waste, and to the best of our knowledge, we obtained the highest production of folate from residual biomasses ever reported, providing the first indications for the future development of this microbial production process.
Reproductive factors and metabolic syndrome among Chinese women aged 40 years and older
Authors: Bai Ling , Yang Xi , Sun Ziyi , Luo Zuojie , Li Li , Liang Xinghuan , Zhou Jia , Meng Liheng , Peng Yang , Qin Yingfen 
Published: December 2022

AbstractBackgroundThe aim of this study is to explore the relationship between reproductive variables and the prevalence of metabolic syndrome (MetS) and its components among Chinese women aged 40 years and older.MethodsA cross‐sectional study was conducted among 4453 women aged 40 years and older in Guangxi, China. The associations between women's reproductive factors and MetS were analyzed using a logistic regression model.ResultsThe prevalence of MetS was 23.9% in this population. Women with MetS were mostly older, more likely to be postmenopausal, and had higher parity. Compared to women with one prior live birth, those with three or more live births had the highest odds of having MetS (odds ratio [OR] = 1.56; 95% CI, 1.23–1.99). Similarly, compared to premenopausal women, postmenopausal participants had higher odds of having MetS (OR = 1.86; 95% CI, 1.49–2.31). No associations were observed between MetS and abortion or with age at menarche.ConclusionsOur study suggests that multiparity and menopausal status may be associated with the development of MetS. The inconsistency seen in epidemiological research to date calls for further investigation.
Developing a prediction model for all‐cause mortality risk among patients with type 2 diabetes mellitus in Shanghai, China
Authors: Qi Jiying , He Ping , Yao Huayan , Xue Yanbin , Sun Wen , Lu Ping , Qi Xiaohui , Zhang Zizheng , Jing Renjie , Cui Bin , Ning Guang 
Published: December 2022

AbstractBackgroundAll‐cause mortality risk prediction models for patients with type 2 diabetes mellitus (T2DM) in mainland China have not been established. This study aimed to fill this gap.MethodsBased on the Shanghai Link Healthcare Database, patients diagnosed with T2DM and aged 40‐99 years were identified between January 1, 2013 and December 31, 2016 and followed until December 31, 2021. All the patients were randomly allocated into training and validation sets at a 2:1 ratio. Cox proportional hazards models were used to develop the all‐cause mortality risk prediction model. The model performance was evaluated by discrimination (Harrell C‐index) and calibration (calibration plots).ResultsA total of 399 784 patients with T2DM were eventually enrolled, with 68 318 deaths over a median follow‐up of 6.93 years. The final prediction model included age, sex, heart failure, cerebrovascular disease, moderate or severe kidney disease, moderate or severe liver disease, cancer, insulin use, glycosylated hemoglobin, and high‐density lipoprotein cholesterol. The model showed good discrimination and calibration in the validation sets: the mean C‐index value was 0.8113 (range 0.8110–0.8115) and the predicted risks closely matched the observed risks in the calibration plots.ConclusionsThis study constructed the first 5‐year all‐cause mortality risk prediction model for patients with T2DM in south China, with good predictive performance.
Deciphering the role of platelets in severe allergy by an integrative omics approach
Authors: Pablo‐Torres Carmela , Izquierdo Elena , Tan Tiak Ju , Obeso David , Layhadi Janice A. , Sánchez‐Solares Javier , Mera‐Berriatua Leticia , Bueno‐Cabrera José Luis , del Mar Reaño‐Martos María , Iglesias‐Cadarso Alfredo , Barbas Coral , Gomez‐Casado Cristina , Villaseñor Alma , Barber Domingo , Shamji Mohamed H. , Escribese María M. 
Published: December 2022

AbstractBackgroundMechanisms causing the onset and perpetuation of inflammation in severe allergic patients remain unknown. Our previous studies suggested that severe allergic inflammation is linked to platelet dysfunction.MethodsPlatelet‐rich plasma (PRP) and platelet‐poor plasma (PPP) samples were obtained by platelet‐apheresis from severe (n = 7) and mild (n = 10) allergic patients and nonallergic subjects (n = 9) to perform platelet lipidomics by liquid chromatography coupled to mass spectrometry (LC–MS) and RNA‐seq analysis. Significant metabolites and transcripts were used to identify compromised biological pathways in the severe phenotype. Platelet and inflammation‐related proteins were quantified by Luminex.ResultsPlatelets from severe allergic patients were characterized by high levels of ceramides, phosphoinositols, phosphocholines, and sphingomyelins. In contrast, they showed a decrease in eicosanoid precursor levels. Biological pathway analysis performed with the significant lipids revealed the alteration of phospholipases, calcium‐dependent events, and linolenic metabolism. RNAseq confirmed mRNA overexpression of genes related to platelet activation and arachidonic acid metabolism in the severe phenotypes. Pathway analysis indicated the alteration of NOD, MAPK, TLR, TNF, and IL‐17 pathways in the severe phenotype. P‐Selectin and IL‐17AF proteins were increased in the severe phenotype.ConclusionsThis study demonstrates that platelet lipid, mRNA, and protein content is different according to allergy severity. These findings suggest that platelet load is a potential source of biomarkers and a new chance for therapeutic targets in severe inflammatory pathologies.
Predictors of outcome after catheter ablation for atrial fibrillation: Group analysis categorized by age and type of atrial fibrillation
Authors: Uemura Tetsuya , Kondo Hidekazu , Sato Hiroki , Takahashi Masaki , Shinohara Tetsuji , Mitarai Kazuki , Fukui Akira , Hirota Kei , Fukuda Tomoko , Kodama Nozomi , Miyoshi Miho , Ogawa Naoko , Wada Masato , Yamasaki Hirochika , Iwanaga Kenzo , Uno Akihiro , Tawara Katsunori , Yonezu Keisuke , Akioka Hidefumi , Teshima Yasushi , Yufu Kunio , Nakagawa Mikiko , Takahashi Naohiko 
Published: December 2022

AbstractBackgroundThe outcome of catheter ablation could probably differ among patients with atrial fibrillation (AF), depending on age and AF type. We aimed to investigate the difference in predictors of outcome after catheter ablation for AF among the patient categories divided by age and AF type.Methods and ResultsA total of 396 patients with AF (mean age 65.69 ± 11.05 years, 111 women [28.0%]) who underwent catheter ablation from January 2018 to December 2019 were retrospectively analyzed. We divided the patients into four categories: patients with paroxysmal AF (PAF) or persistent AF (PeAF) who were 75 years or younger (≤75 years) or older than 75 years (>75 years). Kaplan–Meier survival analysis demonstrated that patients with PAF aged ≤75 years had the lowest AF recurrence among the four groups (log‐rank test, p = .0103). In the patients with PAF aged ≤75 years (N = 186, 46.7%), significant factors associated with recurrence were female sex (p = .008) and diabetes (p = .042). In the patients with PeAF aged ≤75 years (N = 142, 35.9%), the only significant factor associated with no recurrence was medication with a renin‐angiotensin system inhibitor (p = .044). In the patients with PAF aged >75 years (N = 53, 14.4%), diabetes was significantly associated with AF recurrence (p = .021). No significant parameters were found in the patients with PeAF aged >75 years (N = 15, 4.1%).ConclusionsOur findings indicate that the risk factors for AF recurrence after catheter ablation differed by age and AF type.
Insulating jackets thermally protect kidneys in an ex vivo model of second warm ischemia
Authors: Khan Turaab , Kwarcinski Jeremy , Boughton Philip , Yoon Peter , Hameed Ahmer , Singla Animesh , Pang Tony , Pleass Henry 
Published: December 2022

AbstractBackgroundKidney transplantation is the current optimal treatment for suitable patients with end‐stage renal disease. The second warm ischemic time (SWIT) is known to negatively impact delayed graft function, and long‐term graft survival, and methods are required to ameliorate the impacts of SWIT on transplantation outcomes.Materials and MethodsThis study primarily focused on determining the effect of a novel thermally insulating jacket on the thermal profile of the human kidney and quantifying the reduction in thermal energy experienced using this device (KPJ™). An ex vivo simulated transplantation model was developed to determine the thermal profiles of non‐utilized human kidneys with and without KPJ™ (n = 5). Control kidney temperature profiles were validated against the temperature profiles of n = 10 kidneys during clinical kidney transplantation.ResultsUsing the ex‐vivo water bath model, the thermally insulated human kidney reached the 15°C metabolic threshold temperature at 44.5 ± 1.9 min (vs control: 17.3 ± 1.8 min (p = 0.00172)) and remained within the 18°C threshold until 53.3 ± 1.3 min (vs control: 20.9 ± 2.0 min (p = 0.002)). The specific heat capacity of KPJ™ protected kidney was four‐fold compared to the control kidney. The clinical temperature audit, closely correlated with the water bath model, hence validating this ex‐vivo human kidney transplant model.ConclusionIntraoperative thermal protection is a simple and viable method of reducing the thermal injury that occurs during the SWIT and increasing the specific heat capacity of the system. Such technology could easily be translated into clinical kidney transplant practice.
Cooling strategies during neonatal transport for hypoxic‐ischaemic encephalopathy
Authors: Sibrecht Greta , Borys Franciszek , Campone Chiara , Bellini Carlo , Davis Peter , Bruschettini Matteo 
Published: December 2022

AbstractAimWe reviewed the literature on cooling methods during transport of newborn infants with hypoxic‐ischaemic encephalopathy (HIE) born in a non‐tertiary centre and transferred to a neonatal intensive care unit for therapeutic hypothermia.MethodsThe electronic databases CENTRAL, MEDLINE, Embase, CINAHL, and Scopus were searched from inception up to 8 March 2022 for studies comparing cooling versus no cooling, active versus passive cooling, and servo‐controlled versus non‐servo‐controlled cooling. Odds ratio and confidence of interval were calculated for dichotomous outcomes and mean difference and confidence interval for continuous outcomes.ResultsThe final analysis included 14 studies, 1 randomised and 13 non‐randomised, involving 1098 newborn infants. Compared with the other cooling methods, servo‐controlled active cooling was more likely to maintain body temperature within the target range of 33°C–34°C on arrival at a neonatal intensive care unit: odds ratio 13.58, 95% confidence interval 4.32–42.66, risk difference 0.33, 95% confidence interval 0.19–0.46; 224 participants; three studies; I2 0%. The certainty of evidence was low. Only five studies reported mortality rates.ConclusionServo‐controlled active cooling may be the preferred method during transport of newborn infants with HIE. A future area of focus should be long‐term neurodevelopmental outcomes after servo‐controlled active cooling.
Gratitude intersects with affect as a boundary condition for daily satisfaction: An affective dynamics perspective
Authors: Mosteo Leticia , Junça‐Silva Ana , Lopes Rita Rueff 
Published: December 2022

AbstractThis study is based on the affective events theory to investigate the situational predictors for gratitude‐related differences in daily affect and satisfaction. We tested a moderated mediation model in which daily microevents (daily hassles and uplifts) were related to satisfaction through affect, at the within‐person level. We also tested the cross‐level interaction of gratitude on this indirect relationship. A total of 195 participants participated in a 5‐day diary study (195 * 5 = 975 measurement occasions). Multilevel modeling showed that, at the person‐level of analysis, daily microevents were significantly related to daily affect and, in turn, to daily satisfaction. At the daily level of analysis, trait‐based gratitude moderated the mediation of daily positive affect on the relationship between daily uplifts and daily satisfaction, such that it become stronger for individuals who scored lower on gratitude, but gratitude did not moderate the relationship between daily hassles, negative affect, and satisfaction. These findings make relevant theoretical contributions to understanding the power of gratitude for daily affective dynamics. These results also expand knowledge on within‐person processes that explain daily affect and satisfaction, in addition to more traditional between‐person factors. In sum, the present research demonstrates that “being grateful” may be associated with being happy and that individuals who are less grateful need to experience more daily uplifts and positive affect to feel satisfied.
Monks on the move? An assessment of mobility at the medieval Nubian monastery of Ghazali, Sudan (ca. 680–1,275 CE)
Authors: Stark Robert J. , Ciesielska Joanna A. , Obłuski Artur 
Published: December 2022

AbstractThe location of Ghazali monastery away from the Nile valley within the relatively isolated environs of the Bayuda desert presents a landscape suggestive of mobility toward the monastery by those who chose to reside there as monks. To assess this potentiality, a sample of 37 individuals from the monastic cemetery (Cemetery 2) were analysed for 87Sr/86Sr and δ18O to assess residency during dental enamel formation. The data generated bring into question the nature of mobility to Ghazali monastery, particularly in regard to the potential movement of people from the Nile valley, adjacent desertic landscapes, and further afield.
Reconstruction of diets based on the Δ15N values of individual amino acids at three sites in Sichuan, China
Authors: Lin Kuei‐chen , Lee Cheng‐Yi , Itahashi Yu , Zhou Zhiqing , Chen Jian , Liu Xiangyu , Yoneda Minoru 
Published: December 2022

AbstractThis study analyzes the nitrogen isotope composition of individual amino acids in collagen extracted from human bone samples from the Gaoshan, Yingpanshan, and Xinyicun sites in Sichuan, where the isotope baseline is unavailable or incomparable with the isotopic data derived from human remains. It aims to understand the food compositions of the inhabitants at these sites, spanning the period from the late Neolithic to the middle Bronze Age, during which time agriculture and domestication of animals were introduced to the region and became increasingly important. The δ15N values of two amino acids, phenylalanine (δ15NPhe) and glutamic acid (δ15NGlu), indicate that the peoples on the Chengdu Plain mainly consumed terrestrial foods. The contribution of aquatic resources to their diet was limited. A possible explanation for the low dependence on aquatic foods is that the developed agriculture and domestication of animals offered sufficient foods. Such subsistence economies and dietary patterns were shaped in the early Baodun period (ca. 2,500–2000 BCE) at the latest and did not seem to change when transiting to the Bronze Age. This study also assesses the significance of these subsistence practices in supporting the social development of the Chengdu Plain.
Combined targeting of fatty acid amide hydrolase and melatonin receptors promotes neuroprotection and stimulates inflammation resolution in rats
Authors: Cammarota Mariarosaria , Ferlenghi Francesca , Vacondio Federica , Vincenzi Fabrizio , Varani Katia , Bedini Annalida , Rivara Silvia , Mor Marco , Boscia Francesca 
Published: December 2022

Background and PurposeDevising novel strategies to therapeutically favour inflammation resolution and provide neuroprotection is an unmet clinical need. Enhancing endocannabinoid tone by inhibiting the catabolic enzyme fatty acid amide hydrolase (FAAH), or stimulating melatonin receptors has therapeutic potential to treat neuropathological states in which neuroinflammation plays a central role.Experimental ApproachA rodent hippocampal explant model of inflammatory injury was used to assess the effects of UCM1341, a dual‐acting compound with FAAH inhibitory action and agonist activity at melatonin receptors, against neuroinflammatory damage. FAAH activity was measured by a radiometric assay, and N‐acylethanolamine levels were assessed by HPLC–MS/MS methods. FAAH distribution, evolution of inflammation and the contribution of UCM1341 to the expression of proteins controlling macrophage behaviour were investigated by biochemical and confocal analyses.Key ResultsUCM1341 exhibited greater neuroprotection against neuroinflammatory degeneration, compared with the reference compounds URB597 (FAAH inhibitor) and melatonin. During neuroinflammation, UCM1341 augmented the levels of anandamide and N‐oleoylethanolamine, but not N‐palmitoylethanolamine, up‐regulated PPAR‐α levels, attenuated demyelination and prevented the release of TNF‐α. UCM1341 modulated inflammatory responses by contributing to microglia/macrophage polarization, stimulating formation of lipid‐laden macrophages and regulating expression of proteins controlling cholesterol metabolism and efflux. The neuroprotective effects of UCM1341 were prevented by PPARα, TRPV1 and melatonin receptor antagonists.Conclusion and ImplicationsUCM1341, by enhancing endocannabinoid and melatoninergic signalling, benefits neuroprotection and stimulates inflammation resolution pathways. Our findings provide an encouraging prospect of therapeutically targeting endocannabinoid and melatoninergic systems in inflammatory demyelinating states in the CNS.
Adolescents' problematic internet and smartphone use in (cyber)bullying experiences: A network analysis
Authors: Blinka Lukas , Stašek Andrea , Šablatúrová Nika , Ševčíková Anna , Husarova Daniela 
Published: December 2022

BackgroundThis study investigates both offline and online bullying perpetration and victimization in association with problematic internet use (PIU) and problematic smartphone use (PSU), while also considering the related psychosocial difficulties.MethodsA total of 3939 adolescents (49.4% boys, aged 13–15 years) from a representative sample of schools in Slovakia was obtained from the Health Behavior in School‐aged Children (HBSC) project in 2018. Due to the lack of similar complex research and the expected mutual associations among the constructs, we compared exploratory network models that provided separate estimates for boys and girls.ResultsThe constructs formed similarly clustered networks for both genders, with a few notable differences. Unlike PIU, PSU was not associated with bullying experiences in boys or girls; however, PSU and PIU were partially related in girls. Bullying experiences formed a strong cluster in both networks. Two strong bridges were identified, and they are potential candidates for intervention in both boys and girls: first, traditional bullying victimization connected the cluster of bullying experiences to psychosocial difficulties; and, second, frequently missing sleep or meals due to internet use (behavioral salience) that is connected to the bullying cluster with PIU.ConclusionsThe findings offer an indication for the preventive and interventive work of practitioners who deal with adolescents, as well as complex gender comparisons for the mutual relations of problematic internet and smartphone use, bullying experiences, and the psychosocial difficulties of youth. This study provides evidence that problematic digital media can play a role in bullying experiences irrespective of whether bullying happens offline or online.
Icaritin inhibits oxidative stress in murine astrocytes by binding to Orai1 to block store‐operated calcium channel
Authors: Fan Jianwei , Miao Yu , Zhao Yun , Guan Yongxia , Zhang Li , Pan Lihong , Feng Qun , Yao Jingchun , Sun Chenghong 
Published: December 2022

AbstractPrevious study has shown that icaritin (ICT) has meaningful protective effect on cerebral ischemic stroke, and this study aimed to investigate its mechanism from the aspect of protecting astrocytes from oxidative stress. Murine primary astrocytes were pretreated by ICT and exposed to H2O2 to induce oxidative stress. The results indicated that ICT inhibited H2O2‐induced astrocytes apoptosis, decreased Bax and cleaved caspase‐3, and increased Bcl‐2. In addition, ICT inhibited H2O2‐induced oxidative stress, increased mitochondrial membrane potential (ΔΨm), and maintained mitochondrial morphology. ICT decreased the synthesis of malondialdehyde and increased the activity of glutathione peroxidase, catalase, and superoxide dismutase. Moreover, ICT suppressed the transient and resting intracellular Ca2+ overload. Further investigation revealed that ICT could target the combination with Orai1 to block store‐operated calcium channel induced by H2O2. However, ICT did not enhance the protective effect of RO2959, a selective blocker of Orai1. These results indicate that ICT can play a neuroprotective role against oxidative stress injury by binding to Orai1 to block SOCC.