bluuebunny/crossref_metadata_2025_split huggingface dataset:
ALDOC- and ENO2- driven glucose metabolism sustains 3D tumor spheroids growth regardless of nutrient environmental conditions: a multi-omics analysis
Authors: De Vitis Claudia , Battaglia Anna Martina , Pallocca Matteo , Santamaria Gianluca , Mimmi Maria Chiara , Sacco Alessandro , De Nicola Francesca , Gaspari Marco , Salvati Valentina , Ascenzi Francesca , Bruschini Sara , Esposito Antonella , Ricci Giulia , Sperandio Eleonora , Massacci Alice , Prestagiacomo Licia Elvira , Vecchione Andrea , Ricci Alberto , Sciacchitano Salvatore , Salerno Gerardo , French Deborah , Aversa Ilenia , Cereda Cristina , Fanciulli Maurizio , Chiaradonna Ferdinando , Solito Egle , Cuda Giovanni , Costanzo Francesco , Ciliberto Gennaro , Mancini Rita , Biamonte Flavia 
Published: March 2023

Abstract
Background
Metastases are the major cause of cancer-related morbidity and mortality. By the time cancer cells detach from their primary site to eventually spread to distant sites, they need to acquire the ability to survive in non-adherent conditions and to proliferate within a new microenvironment in spite of stressing conditions that may severely constrain the metastatic process. In this study, we gained insight into the molecular mechanisms allowing cancer cells to survive and proliferate in an anchorage-independent manner, regardless of both tumor-intrinsic variables and nutrient culture conditions.

Methods
3D spheroids derived from lung adenocarcinoma (LUAD) and breast cancer cells were cultured in either nutrient-rich or -restricted culture conditions. A multi-omics approach, including transcriptomics, proteomics, and metabolomics, was used to explore the molecular changes underlying the transition from 2 to 3D cultures. Small interfering RNA-mediated loss of function assays were used to validate the role of the identified differentially expressed genes and proteins in H460 and HCC827 LUAD as well as in MCF7 and T47D breast cancer cell lines.

Results
We found that the transition from 2 to 3D cultures of H460 and MCF7 cells is associated with significant changes in the expression of genes and proteins involved in metabolic reprogramming. In particular, we observed that 3D tumor spheroid growth implies the overexpression of ALDOC and ENO2 glycolytic enzymes concomitant with the enhanced consumption of glucose and fructose and the enhanced production of lactate. Transfection with siRNA against both ALDOC and ENO2 determined a significant reduction in lactate production, viability and size of 3D tumor spheroids produced by H460, HCC827, MCF7, and T47D cell lines.

Conclusions
Our results show that anchorage-independent survival and growth of cancer cells are supported by changes in genes and proteins that drive glucose metabolism towards an enhanced lactate production. Notably, this finding is valid for all lung and breast cancer cell lines we have analyzed in different nutrient environmental conditions. broader Validation of this mechanism in other cancer cells of different origin will be necessary to broaden the role of ALDOC and ENO2 to other tumor types. Future in vivo studies will be necessary to assess the role of ALDOC and ENO2 in cancer metastasis.
Earth-friendly-assessed silver-nanoparticles spectrophotometric method for rapid and sensitive analysis of Molnupiravir, an FDA-approved candidate for COVID-19: application on pharmaceutical formulation and dissolution test
Authors: Mohamed Ahmed R. , Abolmagd Ebrahim , Nour Israa M. , Badrawy Mohamed , Hasan Mohamed A. 
Published: March 2023

AbstractMolnupiravir is the first oral direct-acting antiviral prodrug recently approved for the COVID-19 pandemic. Here and for the first time, we present a novel, sensitive, robust, and simple silver-nanoparticles spectrophotometric technique for molnupiravir analysis in its capsules and dissolution media. This spectrophotometric technique involved silver-nanoparticles synthesis through a redox reaction between the reducing agent (molnupiravir) and the oxidizing agent (silver nitrate) in presence of polyvinylpyrrolidone as a stabilizing agent. The produced silver-nanoparticles have an intense surface plasmon resonance peak at 416 nm where the measured absorbance values were utilized for the quantitative analysis of molnupiravir. The produced silver-nanoparticles were recognized by using the transmission electron microscope. Under optimal conditions, a good linear rapport was accomplished between molnupiravir concentrations and the corresponding absorbance values in a range of (100–2000) ng/mL with a detection limit of 30 ng/mL. Greenness assessment was implemented using eco-scale scoring and GAPI disclosing the excellent greenness of the suggested technique. The suggested silver-nanoparticles technique was authenticated according to recommendations of the ICH and statistically assessed with the reported liquid chromatographic method without significant differences regarding accuracy or precision. Accordingly, the suggested technique is deemed a green and cheap alternative for assaying molnupiravir due to its reliance primarily on water. Furthermore, the suggested technique’s high sensitivity can be employed for investigating molnupiravir bioequivalence in future studies.
Graphical Abstract
Screening for diabetic peripheral neuropathy in resource-limited settings
Authors: Nkonge Ken Munene , Nkonge Dennis Karani , Nkonge Teresa Njeri 
Published: March 2023

AbstractBackgroundDiabetic neuropathy is the most common microvascular complication of diabetes mellitus and a major risk factor for diabetes-related lower-extremity complications. Diffuse neuropathy is the most frequently encountered pattern of neurological dysfunction and presents clinically as distal symmetrical sensorimotor polyneuropathy. Due to the increasing public health significance of diabetes mellitus and its complications, screening for diabetic peripheral neuropathy is essential. Consequently, a review of the principles that guide screening practices, especially in resource-limited clinical settings, is urgently needed.Main bodyNumerous evidence-based assessments are used to detect diabetic peripheral neuropathy. In accordance with current guideline recommendations from the American Diabetes Association, International Diabetes Federation, International Working Group on the Diabetic Foot, and National Institute for Health and Care Excellence, a screening algorithm for diabetic peripheral neuropathy based on multiphasic clinical assessment, stratification according to risk of developing diabetic foot syndrome, individualized treatment, and scheduled follow-up is suggested for use in resource-limited settings.ConclusionsScreening for diabetic peripheral neuropathy in resource-limited settings requires a practical and comprehensive approach in order to promptly identify affected individuals. The principles of screening for diabetic peripheral neuropathy are: multiphasic approach, risk stratification, individualized treatment, and scheduled follow-up. Regular screening for diabetes-related foot disease using simple clinical assessments may improve patient outcomes.
Two cases of transplant-acquired food allergy who developed resensitization after a negative oral food challenge
Authors: Nakaoka Akiko , Nomura Takayasu , Ozeki Kazuyoshi , Suzuki Tomotaka , Kusumoto Shigeru , Iida Shinsuke , Saitoh Shinji 
Published: March 2023

Abstract
Background
Cases of food allergy after hematopoietic stem cell and solid organ transplantation in previously nonallergic transplant recipients were reported as transplant-acquired food allergy (TAFA), but information about its long-term outcome is still limited. A phenomenon where patients reacquire food allergy by resuming daily consumption after a negative oral food challenge has not yet been reported.

Case presentation
We report two cases of TAFA after liver transplantation and cord blood transplantation. In each case, the threshold of daily consumption to cause allergic symptoms decreased when a negative oral food challenge was obtained.

Conclusions
Our cases show an importance of gastrointestinal tract as a route of food sensitization because thresholds that caused allergic reactions decreased during their resuming process. We need to be careful with possible resensitization once a negative substantial dose was confirmed.
Imaging the TGFβ type I receptor in pulmonary arterial hypertension
Authors: Rotteveel Lonneke , Poot Alex J. , Kooijman Esther J. M. , Schuit Robert C. , Schalij Ingrid , Sun Xiaoqing , Kurakula Kondababu , Happé Chris , Beaino Wissam , ten Dijke Peter , Lammertsma Adriaan A. , Bogaard Harm Jan , Windhorst Albert D. 
Published: March 2023

AbstractTransforming growth factor β (TGFβ) activity is perturbed in remodelled pulmonary vasculature of patients with pulmonary arterial hypertension (PAH), cancer, vascular diseases and developmental disorders. Inhibition of TGFβ, which signals via activin receptor-like kinase 5 (ALK5), prevents progression and development of experimental PAH. The purpose of this study was to assess two ALK5 targeting positron emission tomography (PET) tracers ([11C]LR111 and [18F]EW-7197) for imaging ALK5 in monocrotaline (MCT)- and Sugen/hypoxia (SuHx)-induced PAH. Both tracers were subjected to extensive in vitro and in vivo studies. [11C]LR111 showed the highest metabolic stability, as 46 ± 2% of intact tracer was still present in rat blood plasma after 60 min. In autoradiography experiments, [11C]LR111 showed high ALK5 binding in vitro compared with controls, 3.2 and 1.5 times higher in SuHx and MCT, respectively. In addition, its binding could be blocked by SB431542, an adenosine triphosphate competitive ALK5 kinase inhibitor. However, [18F]EW-7197 showed the best in vivo results. 15 min after injection, uptake was 2.5 and 1.4 times higher in the SuHx and MCT lungs, compared with controls. Therefore, [18F]EW-7197 is a promising PET tracer for ALK5 imaging in PAH.
Genotypic and phenotypic characterization of glucose-6-phosphate dehydrogenase (G6PD) deficiency in Guangzhou, China
Authors: Li Ziyan , Huang Zhenyi , Liu Yanxia , Cao Yunshan , Li Yating , Fang Yanping , Huang Meiying , Liu Zixi , Lin Lijuan , Jiang Lingxiao 
Published: March 2023

Abstract
Background
G6PD deficiency is a common inherited disorder worldwide and has a higher incidence rate in southern China. Many variants of G6PD result from point mutations in the G6PD gene, leading to decreased enzyme activity. This study aimed to analyse the genotypic and phenotypic characteristics of G6PD deficiency in Guangzhou, China.

Methods
In this study, a total of 20,208 unrelated participants were screened from 2020 to 2022. G6PD deficiency was further analysed by quantitative enzymatic assay and G6PD mutation analysis. The unidentified genotype of the participants was further ascertained by direct DNA sequencing.

Results
A total of 12 G6PD mutations were identified. Canton (c.1376G>T) and Kaiping (c.1388G>A) were the most common variants, and different mutations led to varying levels of G6PD enzyme activity. Comparing the enzyme activities of the 6 missense mutations between the sexes, we found significant differences (P < 0.05) in the enzyme activities of both male hemizygotes and female heterozygotes. Two previously unreported mutations (c.1438A>T and c.946G>A) were identified.

Conclusions
This study provided detailed genotypes of G6PD deficiency in Guangzhou, which could be valuable for diagnosing and researching G6PD deficiency in this area.
More than an outcome: a person-centered, ecological framework for eating disorder recovery
Authors: Kenny Therese E. , Lewis Stephen P. 
Published: March 2023

AbstractBackgroundEating disorder recovery is a complex phenomenon. While historical understandings focused on weight and behaviours, the importance of psychological factors is now widely recognized. It is also generally accepted that recovery is a non-linear process and is impacted by external factors. Recent research suggests a significant impact of systems of oppression, though these have not yet been named in models of recovery.BodyIn this paper, we propose a research-informed, person-centered, and ecological framework of recovery. We suggest that there are two foundational tenets of recovery which apply broadly across experiences: recovery is non-linear and ongoing and there is no one way to do recovery. In the context of these tenets, our framework considers individual changes in recovery as determined by and dependent on external/personal factors and broader systems of privilege. Recovery cannot be determined by looking solely at an individual’s level of functioning; one must also consider the broader context of their life in which changes are being made. To conclude, we describe the applicability of the proposed framework and offer practical considerations for incorporating this framework in research, clinical, and advocacy settings.
Trad Rights: Making Eurasian Whiteness at the “End of History”
Authors: Feldman Leah 
Published: March 2023

AbstractThis essay addresses how the collapse of the Soviet Union and the conditions of social, political, and economic precarity that followed gave rise to intertwining strands of global New Right thought. Taking up the Russian Right's revanchist-revolutionary vision of neo-Eurasianism and its attendant imaginary of a white Eurasian statehood comparatively in relation to US and Hungarian New Right thought, the essay exposes how the neotraditionalist (Trad right) generated a global political and intellectual project in counterpoint to the failed leftist internationalist projects of the twentieth century. The essay closes with a postscript reflection on how the invasion of Ukraine constitutes a major step toward the Trad right's reclaiming of a “multipolar” New Right world order.
The White Minority: Natives and Nativism in Contemporary France
Authors: Harrison Olivia C. 
Published: March 2023

AbstractIn 2009, a nativist association named AGRIF sued antiracist activist Houria Bouteldja for “anti-white racism.” Though unsuccessful in court, the legal proceedings against Bouteldja are illustrative of a decades-long phenomenon that has accelerated in the age of new media: the recuperation of antiracist discourses by nativist activists who claim that white people constitute a minority in France. This article tracks the emergence of French nativist discourses from the nineteenth century to the present, with particular attention to identification with the figure of the colonized in the colonial archive and in the nativist discourses that have accompanied decolonization in France. Anchored in the history of France's prized settler colony, Algeria, the colonial genealogy of French nativism offers lessons for the study of nativism in other (post)colonial contexts.
Response evaluation of two commercial thermoluminescence dosimeters (TLDs) against different parameters
Authors: Alanazi Sitah Fahad , Alarifi Haya , Alshehri Abdullah , Almurayshid Mansour 
Published: March 2023

Objectives: It is essential to study the dosimetric performance and reliability of personal dosimeters. This study examines and compares the responses of two commercial thermoluminescence dosimeters (TLDs), the TLD-100 and the MTS-N. Methods: We compared the two TLDs to various parameters such as energy dependence, linearity, homogeneity, reproducibility, light sensitivity (zero point), angular dependence, and temperature effects using the IEC 61066 standard. Results: The results acquired showed that both TLD materials show linear behavior as indicated by the quality of the ﬁt. In addition, the angular dependence results for both detectors show that all dose responses are within the range of acceptable values. However, the TLD-100 outperformed the MTS-N in terms of light sensitivity reproducibility for all detectors together, while the MTS-N outperforms the TLD-100 for each detector independently and that showed TLD-100 has more stability than MTS-N. The MTS-N shows better batch homogeneity (10.84%) than TLD-100 (13.65%). The effect of temperature in signal loss was clearer at higher temperature 65°C and it was however below ±30%. Conclusions: The overall results for dosimetric properties determined in terms of dose equivalents for all combinations of detectors are satisfactory. The MTS-N cards have better results in the energy dependence, angular dependency, batch homogeneity and less signal fading, whereas the TLD-100 cards are less sensitive to light and more reproducible. Advances in knowledge: Although previous studies showed several types of comparisons between TLDs, they have used limited parameters and different data analysis. This study has dealt with more comprehensive characterization methods and examinations combining TLD-100 and MTS-N cards.
Seroprevalence of severe fever with thrombocytopenia syndrome using specimens from the Korea National Health & Nutrition Examination Survey
Authors: Kim Choon-Mee , Han Mi Ah , Yun Na Ra , Bang Mi-Seon , Lee You Mi , Lee Beomgi , Kim Dong-Min 
Published: March 2023

Background
Severe fever with thrombocytopenia syndrome (SFTS) is an acute febrile disease caused by bites from ticks infected with the SFTS virus. In Korea, SFTS patients are observed nationwide, including Jeju Island, but there are currently no data regarding the national prevalence of SFTS, including that of residents of 16 cities and provinces. This study aimed to investigate the seroprevalence of SFTS in Korea.

Methodology/Principal findings
A total of 1500 participants were selected through random sampling from the 2014–2015 Korea National Health and Nutrition Examination Survey (KNHANES). An indirect immunofluorescence antibody assay (IFA) was performed to assess immunoglobulin (Ig) G and IgM antibody titers against SFTS virus.

Results
Of the 1500 participants, 55 (3.7%) tested positive for IgG and 1 (0.1%) tested positive for IgM, with antibody titer of ≥ 1:32. Approximately 3.9% and 2.5% of participants in urban and rural areas, respectively, had a positive titer of ≥ 1:32. There was a significant correlation between SFTS incidence per 100,000 population and seroprevalence using an IgG titer ≥ 1:64 as the cut-off value.

Conclusion
This is the first study to investigate national SFTS seroprevalence in all 16 cities and provinces representing Korea. Our study will also provide useful guidelines for the development of preventive measures against SFTS.
Exploratory and confirmatory factor analysis of the new region-generic version of Fremantle Body Awareness—General Questionnaire
Authors: Walton David M. , Nazari Goris , Bobos Pavlos , MacDermid Joy C. 
Published: March 2023

Background
As the field of pain evaluation grows, newer and more targeted tools are being published for patient-centric evaluation of specific aspects of the pain experience. The Fremantle Back Awareness Questionnaire (FreBAQ) is intended to capture alterations in bodily awareness or perception. To date only region-specific (back, neck, shoulder, knee) versions have been published.

Objectives
The purpose of our study was to report on the properties of a new region-generic version of the FreBAQ, the FreBAQ-general. Structural validity, internal consistency, and convergent validity against external criteria were evaluated in a sample of Canadian military veterans with chronic pain, with results compared against those published for the region-specific FreBAQ versions.

Methods
Eligible participants were those that had prior military service, were at least 18 years of age and self-identified as having chronic pain. We used a split-sample approach to Exploratory Factor Analysis (EFA) and Confirmatory Factor Analysis (CFA) on independent random samples. Factor structure, internal consistency, and associations with external criteria were used to compare against prior versions.

Results
328 respondents (74% of consented) completed at least 7 of the 9 FreBAQ-general questions. EFA and CFA on two independent samples offered support for both 6- and 7-item versions. Comparisons against the external criteria (pain severity, interference, catastrophizing) indicated no statistical superiority of one over the other, so in the interest of parsimony the 6-item FreBAQ-general was endorsed.

Conclusions
The Fremantle Body Awareness Questionnaire (FreBAQ-general) showed psychometric properties very much in alignment with those previously reported for the region-specific versions, and sound factorial validity accomplished with fewer items (6 vs. 9). We believe this version can be implemented in practice for those seeking a shorter scale without the need to have multiple region-specific versions on hand, though suggest that those seeking direct comparability with previously published work will still wish to use the original versions.
Feeding practices, dietary adequacy, and dietary diversities among caregivers with under-five children: A descriptive cross-section study in Dodoma region, Tanzania
Authors: Millanzi Walter C. , Herman Patricia Z. , Ambrose Bariki A. 
Published: March 2023

Introduction
Suboptimal feeding practices among caregivers contribute to nutritional-related health problems in children in low and middle-income countries, such as Tanzania. The continuum of the recommended feeding practices has been an utmost challenge among caregivers during the transition from health facilities to homes for improved under-five children’s health.

Objective
This study assessed feeding practices, dietary diversities, and dietary adequacy among caregivers with under-five children in the Dodoma region, Tanzania.

Methods
A community-based descriptive cross-sectional study in a quantitative research approach was adopted to study 289 randomly selected caregivers of under-five children in Dodoma region, Tanzania from March to August 2022. The World Health Organization Infant and Young Children Feeding Guidelines was the main data collection tool. Data were analyzed descriptively using a Statistical Package for Social Sciences computer software program version 25. Statistical limits were set at a 95% confidence interval and a 5% significance level.

Results
Mean age was 26±6.47 of which 68.2% (n = 197) were females. Findings revealed 66.1% of caregivers had unsatisfactory feeding practices. It was observed that 67.8% of the caregivers fed their under-five children inadequately. Only 32.2% of them attained the minimum dietary diversity while 35.3% and 31.5% of caregivers demonstrated feeding practices < and > the recommended minimum Dietary diversity respectively. Moreover, 90.7% of caregivers fed their children group one food while 59.1% of them did not practice feeding the children snacks between meals. Feeding practice, dietary diversity, and dietary adequacy were significantly related to the caregiver’s sex, education level, and socioeconomic status (p<0.05).

Conclusion
Under-five children are at serious risk of nutritional problems as most caregivers in this study demonstrated unsatisfactory feeding practices on dietary diversities for dietary adequacy below the world health organization recommended Minimum Dietary diversity and Minimum Meal Frequency. Community-based nutritional education programs for caregivers need to be disseminated to the community level to address the problem accordingly.