bluuebunny/crossref_metadata_2025_split huggingface dataset:
Effect of pregnancy-specific β1-glycoprotein on myeloid-derived suppressor cell differentiation
Authors: Timganova V. P. , Shardina K. Yu. , Bochkova M. S. , Uzhviyuk S. V. , Usanina D. I. , Zamorina S. A. 
Published: June 2023

Myeloid-derived suppressor cells (MDSCs) are a heterogeneous cell population that primarily suppress T lymphocytes in healthy pregnancies and pathologies. MDSCs are one of the key regulators of immune responses. Finding ways to control them is important for the treatment of cancer, autoimmune diseases, miscarriage, and post-transplant complications. The mechanisms of immune suppression by MDSC are: expression of CD73, ADAM17, PD -L1, production of Arg 1, iNOS, IDO, IL -10 and TGF-b1.Pregnancy-specific b1-glycoprotein (PSG) has modulatory effects on dendritic cells and macrophages that mediate the shift of T cell phenotypes toward Th2 and Treg. We have previously shown that native PSG suppresses Th17 differentiation and cytokine production, stimulates the production of IDO by monocytes and the differentiation of Tregs.Considering the immunomodulatory properties of PSG and the key role of MDSCs in pathologies, the aim of our work was to investigate the effect of native and recombinant PSG on the differentiation of MDSCs in vitro.MDSCs were differentiated from CD11b+ peripheral blood cells. Cells were cultured for 7 days and received stepwise GM-CSF, IL-1b, and LPS. Native (n) (1; 10 and 100 mg/mL) and recombinant (r) (1 and 10 mg/mL) PSG were introduced into the cultures three days before the end of incubation. Flow cytometry was used to determine the percentage of MDSC among the cells in culture and the percentage of M-, PMN-, and e-MDSC among the total number of MDSCs.It was found that rPSG (1 mg/mL) increased the percentage of MDSCs in culture. Both nPSG (1 and 10 mg/mL) and rPSG (10 mg/mL) increased the proportion of M-MDSC, whereas rPSG (10 mg/mL) decreased the number of PMN-MDSC.Thus, the cytokine background in CD11b+ cell cultures favored the differentiation of predominantly M-MDSC, similar to the tumor microenvironment, whereas native and recombinant PSG enhanced this effect. Thus, nPSG and rPSG are able to modulate the differentiation of MDSCs by increasing their number, mainly due to the monocytic subpopulation. This fact opens perspectives for new research on targeted manipulation of MDSCs.
Regulation of kisspeptin-54 activity of indolamine-2,3-dioxygenase and apoptosis of peripheral blood lymphocytes
Authors: Gorbunova O. L. , Shirshev S. V. 
Published: June 2023

Pregnancy is a phenomenon of natural semi-allogeneic transplantation, since the fetus is half alien due to the expression of paternal antigens. It was found that the hypothalamic hormone kisspeptin during pregnancy is produced by the syncytiotrophoblast of the placenta and participates in the formation of a new specific hormonal background. Several forms of the hormone circulate in the blood of pregnant women: kisspeptin-10, kisspeptin-14 and kisspeptin-54 (according to the number of amino acid residues in the hormone molecule), but the main active form is kisspeptin-54. The main mechanism for the formation of immune tolerance during pregnancy is the induction of the expression of the enzyme indolamine-2,3- dioxygenase (IDO) by antigen-presenting cells of peripheral blood, resulting in the catalysis of tryptophan (Trp) to kynurenins (KYN) blocking the activation and causing apoptosis of cytotoxic CD8+T lymphocytes in the zone of contact of maternal immune cells with placental-fetal complex antigens. In addition, during pregnancy, an important role is assigned to the process of apoptosis, since activated cells can be potentially dangerous for the developing fetus. Immunocompetent blood cells express a specific membrane receptor of kisspeptin (KISS-1R). Since kisspeptin-54 enters the systemic circulation only during pregnancy, the hormone has an effect on immune cells only during this period.The aim of this work was to evaluate the effect of kisspeptin-54 in concentrations comparable to its levelduring physiological pregnancy on IDO activity and apoptosis of peripheral blood lymphocytes.Peripheral blood mononuclear cells (PBMC) obtained from 10 healthy non-pregnant women of reproductive age (from 23 to 32 years) were used as the object of the study. Lymphocyte apoptosis was assessed in PBMC suspension by staining with annexin-V and propidium iodide. The determination of the number of cells in the early and late stages of apoptosis was carried out in the isolated gate of lymphocytes. IDO activity in PBMC was determined spectrophotometrically by changes in the concentration of KYN, the first stable metabolite of the Trp decay pathway.It was found that kisspeptin-54 at a concentration of 4.6 pM corresponding to the second trimester of pregnancy significantly enhances the activity of IDO, increases the number of cells in the early and late stages of apoptosis. Thus, kisspeptin-54 is an important mechanism for controlling these processes during pregnancy, aimed at protecting the semi-allogeneic fetus from adverse immune reactions of the mother and the favorable development of pregnancy.
Intervenções de urbanismo sensível a água para ampliação da infiltração em área de recarga de aquíferos: Estudo na Cidade do Paranoá - DF
Authors: Santana Jane Cristina do Carmo , Bezerra Maria do Carmo Lima , Oliveira Aline Nóbrega 
Published: June 2023

Afim de promover intervenções em áreas urbanas consolidadas visando ampliar a infiltração, o presente trabalho aborda os serviços ecossistêmicos hídricos e as técnicas de urbanismo sensível à água. O estudo se dá na Região Administrativa (RA) do Paranoá no Distrito Federal, assentada em área de recarga de aquífero. A análise se baseia na pesquisa de Oliveira (2022), que identificou a necessidade de alteração dos padrões urbanos nas RA visando provisão hídrica. Esses foram ajustados com mapeamentos referentes a apropriação de usos dada pela população. Como resultado foram identificadas 36 praças que contam com 39.489,75m² de superfícies impermeável; 3.548,84m² de compactadas/solo exposto e 27.928,44m² de cobertura vegetal. Para intervenção se selecionou 3 praças, onde se propôs: ampliação de áreas gramadas, plantas com folhagem, elementos de acumulação e medidas de conservação da água no solo. Como resultado se obteve aumento de 1.820,32m² para melhoria dos processos de infiltração e, ainda, melhoria no microclima e o conforto térmico local. Das 3 intervenções nas praças do Paranoá, se verificou que a proposta para a praça central se destina somente a ela, devido a sua particularidade, enquanto as outra duas podem ser replicadas nas demais 33 praças, com adaptações, respeitando o dimensionamento específico de cada uma. As intervenções visaram manter a multifuncionalidade, garantindo as diferentes funções esperadas pela comunidade como lazer e interação social associadas a função ecológica hídrica.
PALAVRAS-CHAVE: Áreas Verdes Urbanas. Urbanismo Sensível a Água. Recarga de Aquíferos.
Evaluation of nerve‑sparing radical hysterectomy introduction efficiency in the regional cancer center
Authors: Kolotilov Yu. A. , Kruglov E. A. , Kazantsev A. N. , Unguryan V. M. 
Published: June 2023

The purpose of the study. To evaluate the immediate and long–term results of the introduction of nerve‑s paring radical hysterectomy into the practice of the center, namely, the frequency of complications, duration of surgery, blood loss, genitourinary disorders and compare the data obtained with the data of the world literature on this issue.Patients and methods. In the period from August 2019 to October 2022, 55 patients diagnosed with cervical carcinoma who underwent nerve‑ sparing radical hysterectomy type III/C1 were treated in the conditions of the regional clinical oncological dispensary. The age category ranged from 31 to 69 years, the average age was 47.3 years. The following postoperative indicators were analyzed: duration of surgery, blood loss, amount of residual urine, duration of hospital stay after surgery, complications, mortality, number of lymph nodes examined. The analysis of long‑term oncological results in patients with a follow‑up period of more than 12 months (42–12 months) was carried out, the frequency of relapses, the frequency of progression, and mortality from progression were estimated.Results. The obtained postoperative averages, such as: the duration of the operation, amounted to 286 minutes; blood loss – 216.1 ml, the volume of residual urine on the 5th day 52.4 ml, the duration of hospital stay after surgery 11.1 days, complications of the II degree according to Clavien–Dindo – 3.6 %, complications of the III degree according to Clavien‑ Dindo – 3.6 %, there was no postoperative mortality, the number of lymph nodes examined was 18.3. During the follow‑up period, the recurrence rate was 0 %, relapse‑free survival was 91 %, progression and mortality from progression were 7 % and 3.5 (4 and 2 patients), respectively. The effectiveness of the implementation of the methodology was evaluated by comparing it with the data given in the world literature on a similar issue. At the same time, it was found that the duration of the operation, blood loss, the frequency of complications and genitourinary disorders, the frequency of relapse and progression did not differ significantly from the data of the international literature.Conclusion. The introduction of the technology of nerve‑ sparing hysterectomy of the uterus type III/C1, in cervical cancer surgery is relatively safe and approachable in a regional oncology hospital.
Posthypercapnic syndrome
Authors: Nguyen H. C. , Baranova I. A. , Chuchalin A. G. 
Published: June 2023

Hypercapnic respiratory failure is a common cause of hospitalization and death in patients with chronic obstructive pulmonary disease (COPD).The aim of this work was to analyze the pathophysiological mechanisms and clinical picture of hypercapnic respiratory failure and to discuss the tactics of managing a patient with COPD and posthypercapnic alkalosis that developed during treatment using a clinical case as an example.Conclusion. To date, the search for new treatments for posthypercapnic alkalosis is still relevant, but there are few published studies on this topic. Acute problems in mixed metabolic disorders can be solved with an integrated therapeutic approach that includes the use of medical gases.
Lymphangioleiomyomatosis: diagnosis of a rare cystic lung disease in a clinical case
Authors: Makarova M. A. , Chernyak A V 
Published: June 2023

Sporadic lymphangioleiomyomatosis (LAM) is an orphan disease that primarily affects women of childbearing age. Increasing awareness of this disease among physicians can contribute to its timely diagnosis and treatment.The aim of this work was to present a case of a young woman with a rare cystic lung disease. At the first visit to the clinic, the patient showed pronounced clinical, radiological and functional changes in the bronchopulmonary system, and a rare pathology was suspected. Establishing the definitive diagnosis in the shortest possible time was essential because pathogenetic therapy with an mTOR inhibitor had to be initiated as soon as the diagnosis of LAM had been clarified. Until 2010, a definitive diagnosis of LAM was made only by surgical lung biopsy. However, there is currently a shift in practice toward less invasive diagnostic methods that eliminate the need for surgical intervention in most patients.Conclusion. An algorithm for making a definitive diagnosis (sporadic LAM) without morphologic examination of the lung is demonstrated.
Tracheal stenosis and diaphragm relaxation of post-COVID origin
Authors: Bazarov D. V. , Grigorchuk A. Yu. , Kazaryan G. A. , Abdumuradov K. O. , Kabakov D. G. 
Published: June 2023

Pandemic of the new coronavirus infection has presented the medical community with challenges that call for immediate action. An increase in the number of severe cases of COVID-19 requiring mechanical ventilation inevitably leads not only to an increase in the complication rates, but also to combined complications. A clinical case of a combined tracheal stenosis and diaphragm after severe COVID-19 is presented here.The aim of this publication was to improve treatment outcomes of patients with multiorgan complications of post-COVID origin.Conclusion. Combined complications of a severe coronavirus infection worsen the rehabilitation prognosis, and require surgical treatment.
A Statistical Evolutionary Analysis and Prediction of Carbon-dioxide Emission in Gujarat, Maharashtra and Madhya Pradesh of India
Authors: Dubey Om Prakash , Lal Anamol Kumar , Mondal Kartick , Singh Umashankar 
Published: June 2023

Purpose of the study: The change of climate due to global warming has become a burning problem throughout the world. India is a country where global warming causes a concern to the scientists. Unrestricted emission of different green house gases mainly carbon dioxide is responsible for this alarming situation. This paper develops a state-wise nonlinear emission model of carbon dioxide, an important green house gas.
Methodology: Non linear least-square and regression analysis method used to explain the emission of the gas.
Main Findings: The short term and long-term forecast of carbon dioxide emission trend in the states are presented.
Applications of this study: Model is used in the states of Gujarat, Maharashtra and Madhya Pradesh of India.
Novelty/Originality of this study: Future prediction of emission of CO2 may be evaluated from our proposed model. This paper may be helpful for the future researchers for finding emission of CO2 and other GHGs for the remaining states in India.
АКТУАЛЬНІ ПРОБЛЕМИ ЦИФРОВОЇ ЕТИКИ БІЗНЕСУ
Authors: Компанієць В. В. , Крацер В. В. 
Published: June 2023

В статті наведено визначення сутності цифрової етики бізнесу, основних етичних дилем, проведено системний аналіз проблем та напрямів забезпечення цифрової етики бізнесу на платформі парадигми духовно-моральної та соціокультурної детермінації соціально-економічного розвитку. Розглянуто європейський досвід забезпечення цифрової етики економічних відносин, європейську та вітчизняну законодавчу базу у сфері захисту персональних даних. Висвітлено результати досліджень світових компаній у контексті глобальної кризи довіри до збирачів персональних даних, а також у напрямі забезпечення цифрової відповідальності бізнесу. Сформовано рекомендації щодо посилення захисту прав людини в кіберпросторі та впровадження в освітянський простір та економічну практику етики економічних відносин та бізнесу в умовах цифровізації.
La primera Escuela Normal para Maestras de Párvulos en Guatemala
Authors: Cortez Sic José Enrique , Crispín López Haydee Lucrecia , Hori Herrera Angela Kiku 
Published: June 2023

Objetivo: Analizar el desarrollo histórico de la primera Escuela Normal para Maestras de Párvulos, creada en 1928 en la ciudad Capital de Guatemala, como entidad emblemática en la formación de maestras de preprimaria a nivel nacional.
Originalidad/aporte: este proceso de investigación sigue la línea de la recuperación de la parte histórica y busca visibilizar la experiencia vivida en las diferentes etapas del desarrollo formativo, haciendo hincapié en la evolución curricular y en la importancia de continuar con la formación de calidad para maestras de preprimaria. La historia de las escuelas normales en Guatemala data de 1875 con el inicio de la Escuela Normal Central para Varones. Posteriormente se crearon otras que contribuyeron en la formación de docentes y especialmente la que hoy es objeto de investigación; la Escuela Normal para Maestras de Párvulos “Dr. Alfredo Carrillo Ramírez” institución educativa que fue la primera en su género partir de 1928.
Método: se siguió el método cualitativo con revisión documental, por medio de fuentes primarias, acudiendo a la parte hemerográfica y a una entrevista con un directivo que ha tenido relación directa con el quehacer docente y administrativo de este establecimiento educativo.
Estrategias/recolección de información: se procedió a la revisión de documentos inéditos y bibliografía relacionada con la historia de la Educación en Guatemala, e información base sobre el acontecer de la Escuela Normal a partir de 1928. Así mismo, se procedió a la estructuración de un instrumento para el desarrollo de la entrevista con el personal directivo a partir de preguntas semiestructuradas.
Conclusiones: la primera Escuela Normal para Maestras de Párvulos ha sido un bastión experiencial en el sistema educativo guatemalteco, sus aportes han sido ejemplos valiosos en la formación docente y en la ampliación de cobertura para la atención de niños y niñas del nivel preprimario.
PENERAPAN TEKNOLOGI AUGMENTED REALITY SEBAGAI MEDIA INFORMASI DAN SIMULASI TES PRAKTIK PADA PEMBUATAN SIM C BERBASIS ANDROID
Authors: Mubarok Fadlan Sani , Prasetyo Novian Adi , Wardhana Ariq Cahya 
Published: June 2023

Perkembangan Ilmu Pengetahuan Teknologi (IPTEK) yang pesat membuat jumlah kendaraan sepeda motor di setiap tahunnya mengalami peningkatan. Tercatat dalam kurun waktu 5 tahun terakhir dari 2015 Ã¢â‚¬â€œ 2019 terdapat peningkatan jumlah kendaraan sepeda motor sebanyak 24,12 juta. Dengan adanya hal inimembuat pengendara juga mengalami peningkatan. Sehingga dengan peningkatan tersebut harus diimbangi dengan kepemilikan SIM C agar pengendara dapat memahami peraturan lalu lintas dan terampil mengemudikan kendaraan bermotor. Disisi lain Teknologi Informasi dan Telekomunikasi mengalami kemajuan yang pesat. Salah satunya teknologi yang sedang ramai yaitu Augmented Reality. Oleh karena itu penelitian ini membuat aplikasi yang bertujuan untukmemberikan informasi dan simulasi mengenai tes praktik pada pembuatan SIM C dengan memanfaatkan teknologi Augmented Reality akan jauh lebih mudah. Pembuatan aplikasi ini menggunakan Unity dan Vuforia dengan dengan menggunakan metode Marker Based Tracking. Penelitian ini menggunakan metode MDLC (Multimedia Development Life Cycle) sebagai pengembangan aplikasi Multimedia. Hasil akhir akan ujikan dengan menggunakan uji performansi sistem dan kepuasan pengguna menggunakan metode System Usibility Testing (SUS). Pada metode SUS terdapat 10 pertanyaan dengan 5 opsi jawaban pada setiap pertanyaannya, mulai dari sangat tidak setuju hingga sangat setuju. Pada kuisioner SUS sedikitnya melibatkan 10 orang pengguna sistem. Kata Kunci : Augmented Reality, Unity 3D, SIM, MDLC, System Usibility System.
Exploring pre-service visual art teachers’ competitiveness through porter’s five forces model
Authors: Koshkinbayeva Nurgul , Shagataeva Zaure , Utepova Almagul , Taukebayeva Kunsulu , Kurmantayeva Sulugul 
Published: June 2023

The primary objective of this cross-sectional study was to explore the degree to which 117 pre-service visual art teachers’ competitiveness within the education field. A self-reported competitiveness scale (six items distributed between three factors) was designed and validated by the authors based on the focal points of Michael Porter’s Five Forces framework. Additionally, the respondents’ digital competence was assessed as an auxiliary criterion of educator attractiveness in the art education domain. Both competitiveness and digital skills measurements yielded mediocre scores. Regression analysis showed that respondents’ competitiveness perceptions were predicted neither by digital skills level nor by socio-demographic characteristics such as age, gender, rural or urban background, marital status or whether the student was a graduate or undergraduate. The present study highlights the need for understanding of how visual art majors perceive the sector and evaluate competitiveness among visual art students which itself indicates its sizable contribution to educational research. Other researchers can employ the competitiveness questionnaire introduced to gain deeper insights into the topic or related subjects.
INFLUENCE OF THE COMPOSITION OF THE SYNTHETIZED GAS ON THE COMBUSTION PROCESS OF THE MIXTURE IN THE INTERNAL COMBUSTION ENGINE
Authors: Chríbik Andrej , Poloni Marián , Magdolen Ľuboš , Majkut Andrej , Protasov Roman 
Published: June 2023

The article is devoted to the use of low-energy synthesized gas (synthesis gas) as a fuel for internal combustion engines, which in turn transmit mechanical energy to a cogeneration plant (combined production of heat and electricity). Such power plants allow you to achieve high values of total effective efficiency when using a conventional internal combustion engine with spark ignition. The analysed synthetic gases can be obtained by the method of gasification of household waste with access to air. In this work, synthesis gases were obtained in laboratory conditions. The composition of the components of the studied synthesis gases corresponds to several gas mixtures that are created during the artificial gasification of household waste of certain categories. The influence of the composition of the synthesis gas components on the internal parameters of the internal combustion engine was studied. The process of supplying fuel to the engine was controlled by a standard block with several sensors connected to it in the combustion chamber and the exhaust manifold. Engine operation on all synthesis gas mixtures was compared with operation on pure methane mixture. The analysis shows that the drop in efficiency indicators in the form of indicator mean effective pressure (IMEP) ranges from 10% to 40% compared to work on a pure methane mixture. With an increase in the proportion of hydrogen in the synthesis gas, the stiffness of the engine, as well as the rate of heat generation, increases. When the synthesis gas contained a high proportion of carbon monoxide, the stiffness of the engine was the lowest. The main combustion time of synthesis gas is reduced when hydrogen is added to the mixture, and due to an increase in the proportion of methane or carbon monoxide, it increases on the contrary. The presented results make it possible to analyses the processes occurring in the internal combustion engine, as well as the influence of the components of the synthesis gas produced from renewable energy sources. This will make it possible to adjust the gas production process in such a way as to achieve the highest possible energy and economic indicators of its utilization in the cogeneration plant.
Keywords: internal combustion engine, synthesis gases, analysis of pressure