bluuebunny/crossref_metadata_2025_split huggingface dataset:
Backbone‐Installed Split Intein‐Assisted Ligation for the Chemical Synthesis of Mirror‐Image Proteins
Authors: Zhang Baochang , Zheng Yupeng , Chu Guochao , Deng Xiangyu , Wang Tongyue , Shi Weiwei , Zhou Yongkang , Tang Shan , Zheng Ji‐Shen , Liu Lei 
Published: June 2023

AbstractMembrane‐associated D‐proteins are an important class of synthetic molecules needed for D‐peptide drug discovery, but their chemical synthesis using canonical ligation methods such as native chemical ligation is often hampered by the poor solubility of their constituent peptide segments. Here, we describe a Backbone‐Installed Split Intein‐Assisted Ligation (BISIAL) method for the synthesis of these proteins, wherein the native L‐forms of the N‐ and C‐intein fragments of the unique consensus‐fast (Cfa) (i.e. L–CfaN and L–CfaC) are separately installed onto the two D‐peptide segments to be ligated via a removable backbone modification. The ligation proceeds smoothly at micromolar (μM) concentrations under strongly chaotropic conditions (8.0 M urea), and the subsequent removal of the backbone modification groups affords the desired D‐proteins without leaving any “ligation scar” on the products. The effectiveness and practicality of the BISIAL method are exemplified by the synthesis of the D‐enantiomers of the extracellular domains of T cell immunoglobulin and ITIM domain (TIGIT) and tropomyosin receptor kinase C (TrkC). The BISIAL method further expands the chemical protein synthesis ligation toolkit and provides practical access to challenging D‐protein targets.
Synthesis and Reactivity of a Zinc Diazoalkyl Complex: [3+2] Cycloaddition Reaction with Carbon Monoxide
Authors: Jiang Shengjie , Cai Yanping , Rajeshkumar Thayalan , del Rosal Iker , Maron Laurent , Xu Xin 
Published: June 2023

AbstractThis work reports the synthesis, characterization, and reactivity of the first example of a well‐defined zinc α‐diazoalkyl complex. Treatment of zinc(I)‐zinc(I) bonded compoundL2Zn2[L=CH3C(2,6‐iPr2C6H3N)CHC(CH3)(NCH2CH2PPh2)] or zinc(II) hydrideLZnH with trimethylsilyldiazomethane affords zinc diazoalkyl complexLZnC(N2)SiMe3. This complex liberates N2in the presence of a nickel catalyst to form an α‐zincated phosphorus ylide by reacting with the pendant phosphine. It selectively undergoes formal [3+2] cycloaddition with CO2or CO to form the corresponding product with a five‐membered heterocyclic core. Notably, the use of CO in such a [3+2] cycloaddition reaction is unprecedented, reflecting a novel CO reaction mode.
Characterization and comparison of oleogels and emulgels prepared from Schizochytrium algal oil using monolaurin and MAG/DAG as gelators
Authors: Hyatt Joseph R. , Zhang Siyu , Akoh Casimir C. 
Published: June 2023

AbstractOleogels and emulgels were developed with winterized algal oil from Schizochytrium spp. rich in ω‐3 fatty acids (FAs) to overcome physical limitations of using a highly unsaturated lipid source in food applications. Both gel types were developed using monolaurin or a combination of mono‐ and diacylglycerols (MAG/DAG) as the gelator at concentrations of 8%, 10%, or 12% (w/w) in oil or emulsion. A 30‐day oxidation study was conducted using peroxide value, p‐Anisidine value, and change in FA composition to measure the level of oxidation. Oleogel and emulgel samples exhibited a higher oxidative stability than bulk algal oil and oil‐in‐water emulsion as control groups, respectively. The 12% monolaurin oleogel outperformed others in oxidative stability, preventing oxidation of approximately 11.66% and 7.86% of EPA and DHA, respectively, compared to algal oil. Physical characteristics including thermal behavior, solid fat content (SFC), rheology, morphology, and polymorphism were studied. Results indicated that MAG/DAG oleogels and monolaurin emulgels were the most physically stable. The SFC of 12% MAG/DAG oleogel at 30°C was 10.27% whereas 12% monolaurin oleogel was only 4.51%. Both gel types developed with monolaurin and MAG/DAG could be used for different applications as they exhibited desirable qualities such as oxidative stability and improved physical characteristics.
Investigation of the influence of plasticizers on the biodegradability of cellulose acetate
Authors: Teixeira Samiris Côcco , de Oliveira Taíla Veloso , Fortes‐Da‐Silva Paulo , Raymundo‐Pereira Paulo A. , Ribeiro Alane Rafaela Costa , Batista Lais Fernanda , Gomes Nathalia O. , Stringheta Paulo César , de Fátima Ferreira Soares Nilda 
Published: June 2023

AbstractIn order to evaluate the biodegradability of the cellulose acetate (CA) films plasticized with different concentrations of glycerol (GLY) or triethyl citrate (TEC), analysis of scanning electron microscopy (SEM), Fourier transfer infrared spectroscopy (FTIR), and thermogravimetric analyses (TGA) were conducted through 180 days. Throughout the analysis, indicators of plasticizer exudation were observed, including changes in visual appearance and mass loss, ranging from 39.141% and 20.663% (first day) up to 0.524% and 0.488% (last day) for TEC and GLY plasticizer based films, respectively. Changes were also noted in the infrared spectrum, and TGA plots with polymer degradation peaks from 332.09 to 400.96 cm−1 for both plasticizers based films (TEC and GLY, respectively), regardless of the day of analysis. All assays revealed plasticizer losses, but there was no evidence of films' biodegradation during the experimental time. In perspective, this systematic approach can be extended to study other polymers and other biodegradation conditions.
Sodium‐Dictated Free‐Standing Lignin‐Carbon Electrode towards Ultrahigh Capacitance
Authors: Neupane Manish , Yan Qiangu , He Rui , Masmoudi Rihab , Liu Yifeng , Benedict Zoe , Wang Jinwu , Tripp Carl , Dong Pei , Cai Zhiyong , Yang Yingchao 
Published: June 2023

AbstractLignin is a waste product in the paper industry and lignocellulosic biorefineries, in addition to being the second most abundant renewable biopolymer on Earth. Valorization of useless lignin into high value‐added advanced materials not only helps address the environmentally detrimental biowaste but also satisfies the societal need for energy. While lignin has been converted into porous carbon, made into slurry, and pasted onto metal forms as an electrode for lithium‐ion batteries and supercapacitors, there remains issues with how to scale up the process while achieving great area and mass capacitances in the fabricated lignin‐carbon electrodes. In this work, a thick freestanding electrode coupled by lignin carbon and sodium without any binder and additives was fabricated demonstrating a specific area capacitance of 19.7 F cm−2 at a current density of 1 mA cm−2, which is the highest among to date reported freestanding lignin carbon electrodes with similar thickness. This excellent electrochemical performance originates from high electro‐positivity and oxygen content promoted by the sodium. This work brings a new strategy towards lignin utilization and energy storage through coupling lignin carbon and alkali metals.
Dual gene‐activated dermal scaffolds regulate angiogenesis and wound healing by mediating the coexpression of VEGF and angiopoietin‐1
Authors: Weng Tingting , Yang Min , Zhang Wei , Jin Ronghua , Xia Sizhan , Zhang Manjia , Wu Pan , He Xiaojie , Han Chunmao , Zhao Xiong , Wang Xingang 
Published: June 2023

AbstractThe vascularization of dermal substitutes is a key challenge in efforts to heal deep skin defects. In this study, dual gene‐activated dermal scaffolds (DGADSs‐1) were fabricated by loading nanocomposite particles of polyethylenimine (PEI)/multiple plasmid DNAs (pDNAs) encoding vascular endothelial growth factor and angiopoietin‐1 at a ratio of 1:1. In a similar manner, DGADSs‐2 were loaded with a chimeric plasmid encoding both VEGF and Ang‐1. In vitro studies showed that both types of DGADSs released PEI/pDNA nanoparticles in a sustained manner; they demonstrated effective transfection ability, leading to upregulated expression of VEGF and Ang‐1. Furthermore, both types of DGADSs promoted fibroblast proliferation and blood vessel formation, although DGADSs‐1 showed a more obvious promotion effect. A rat full‐thickness skin defect model showed that split‐thickness skin transplanted using a one‐step method could achieve full survival at the 12th day after surgery in both DGADSs‐1 and DGADSs‐2 groups, and the vascularization time of dermal substitutes was significantly shortened. Compared with the other three groups of scaffolds, the DGADSs‐1 group had significantly greater cell infiltration, collagen deposition, neovascularization, and vascular maturation, all of which promoted wound healing. Thus, compared with single‐gene‐activated dermal scaffolds, DGADSs show greater potential for enhancing angiogenesis. DGADSs with different loading modes also exhibited differences in terms of angiogenesis; the effect of loading two genes (DGADSs‐1) was better than the effect of loading a chimeric gene (DGADSs‐2). In summary, DGADSs, which continuously upregulate VEGF and Ang‐1 expression, offer a new functional tissue‐engineered dermal substitute with the ability to activate vascularization.
In Vitro and Molecular Docking Evaluation of Larvicidal Effects of Essential Oils of Five Aromatic Plants on the Fall Armyworm Spodoptera frugiperda JE. Smith (Lepidoptera: Noctuidae) from Ivory Coast
Authors: Kobenan Koffi Christophe , Kouadio Ibrahime Sinan , Kouakou Malanno , Silvie Pierre , Kouadio Kra Norbert Bini , N'goran Kouadio Emmanuel , Kouakou Brou Julien , Amangoua Nogbou Ferdinand , Tehia Kouakou Etienne , Uba Abdullahi Ibrahim , Zengin Gokhan 
Published: June 2023

AbstractFaced with the serious consequences resulting from the abusive and repeated use of synthetic chemicals, today rethinking crop protection is more than necessary. It is in this context that the essential oils of the Lamiaceae Ocimum gratissimum and Ocimum canum, the Poaceae Cymbopogon citratus and nardus and a Rutaceae Citrus sp. of known chemical compositions were experimented. The evaluation of the larvicidal potential of the essential oils was done by the method of topical application of the test solutions, on the L1−L2 stage larvae from the first generation of S. frugiperda obtained after rearing in an air‐conditioned room. Lethal concentrations (LC10, LC50 and LC90) were determined after 48 h. After assessing the larvicidal potential of essential oils, molecular docking was carried out to study protein‐ligand interactions and their propensity to bind to insect enzyme sites (AChE). The essential oil of O. gratissimum was the most effective with the lowest lethal concentrations (LC10=0.91 %, LC50=1.91 % and LC90=3.92 %). The least toxic oil to larvae was Citrus sp. (LC10=5.44 %, LC50=20.50 % and LC90=77.41 %). Molecular docking revealed that p‐cymene and thymol from O. gratissimum essential oil are structurally similar and bind to the AChE active site via predominantly hydrophobic interactions and a H‐bond with Tyr374 in the case of thymol. The essential oil of O. gratissimum constitutes a potential candidate for the development of biological insecticides for the fight against insect pests and for the protection of the environment.
Experimental and Computational Studies on RuII‐Catalyzed C7‐Allylation of Indolines with Allyl Bromide
Authors: Gupta Shiv Shankar , Gupta Shivangi , Manisha  , Gupta Puneet , Sharma Upendra 
Published: June 2023

AbstractThe selective C7‐allylation of indolines with allyl bromide under ruthenium catalysis has been revealed here. Under established reaction conditions, C7‐allylation of various indolines, including drug compounds, was accomplished with good selectivity and yields. Based on combined experimental and density functional theory (DFT) studies, the olefin insertion route was energetically favorable among four possible pathways. Experimental and DFT studies further revealed that the C−H activation is a reversible rate‐limiting step.
Examining longitudinal markers of bladder cancer recurrence through a semiautonomous machine learning system for quantifying specimen atypia from urine cytology
Authors: Levy Joshua J. , Chan Natt , Marotti Jonathan D. , Rodrigues Nathalie J. , Ismail A. Aziz O. , Kerr Darcy A. , Gutmann Edward J. , Glass Ryan E. , Dodge Caroline P. , Suriawinata Arief A. , Christensen Brock C. , Liu Xiaoying , Vaickus Louis J. 
Published: June 2023

AbstractBackgroundUrine cytology is generally considered the primary approach for screening for recurrence of bladder cancer. However, it is currently unclear how best to use cytological examinations for assessment and early detection of recurrence, beyond identifying a positive finding that requires more invasive methods to confirm recurrence and decide on therapeutic options. Because screening programs are frequent, and can be burdensome, finding quantitative means to reduce this burden for patients, cytopathologists, and urologists is an important endeavor and can improve both the efficiency and reliability of findings. Additionally, identifying ways to risk‐stratify patients is crucial for improving quality of life while reducing the risk of future recurrence or progression of the cancer.MethodsIn this study, a computational machine learning tool, AutoParis‐X, was leveraged to extract imaging features from urine cytology examinations longitudinally to study the predictive potential of urine cytology for assessing recurrence risk. This study examined how the significance of imaging predictors changes over time before and after surgery to determine which predictors and time periods are most relevant for assessing recurrence risk.ResultsResults indicate that imaging predictors extracted using AutoParis‐X can predict recurrence as well or better than traditional cytological/histological assessments alone and that the predictiveness of these features is variable across time, with key differences in overall specimen atypia identified immediately before tumor recurrence.ConclusionsFurther research will clarify how computational methods can be effectively used in high‐volume screening programs to improve recurrence detection and complement traditional modes of assessment.
Practical applications of three‐dimensional printing for process improvement in the cytopathology laboratory
Authors: Monaco Edward A. , Reed Toby , Lynn Terrance J. , Rimini Sarah A. , Patel Aalpen A. , Monaco Sara E. , Patterson Brian S. 
Published: June 2023

AbstractWith the increased availability of three‐dimensional (3D) printers, innovative teaching and training materials have been created in medical fields. For pathology, the use of 3D printing has been largely limited to anatomic representations of disease processes or the development of supplies during the coronavirus disease 2019 pandemic. Herein, an institution's 3D printing laboratory and staff with expertise in additive manufacturing illustrate how this can address design issues in cytopathology specimen collection and processing. The authors' institutional 3D printing laboratory, along with students and trainees, used computer‐aided design and 3D printers to iterate on design, create prototypes, and generate final usable materials using additive manufacturing. The program Microsoft Forms was used to solicit qualitative and quantitative feedback. The 3D‐printed models were created to assist with cytopreparation, rapid on‐site evaluation, and storage of materials in the preanalytical phase of processing. These parts provided better organization of materials for cytology specimen collection and staining, in addition to optimizing storage of specimens with multiple sized containers to optimize patient safety. The apparatus also allowed liquids to be stabilized in transport and removed faster at the time of rapid on‐site evaluation. Rectangular boxes were also created to optimally organize all components of a specimen in cytopreparation to simplify and expedite the processes of accessioning and processing, which can minimize errors. These practical applications of 3D printing in the cytopathology laboratory demonstrate the utility of the design and printing process on improving aspects of the workflow in cytopathology laboratories to maximize efficiency, organization, and patient safety.
Medial–lateral organization of primary auditory cortex and the question of sound localization
Authors: Hallett Mark 
Published: June 2023

AbstractPandya made many important contributions to the understanding of the anatomy of the cortical auditory pathways beginning with his publication in 1969. This review focuses on the observation in that article on the transcallosal connections of the primary auditory cortex. The medial part of the cortex has such connections, but the lateral part does not. Pandya and colleagues speculated that this might have something to do with spatial localization of sound. Review of the subsequent literature shows that the primary auditory cortex anatomy is complex, but the original observation is likely correct. However, the physiological speculation was not.
Long term trends at a comprehensive cancer center during the COVID‐19 pandemic
Authors: Chow Emily C. , Sandercott Nicholas D. , Yoo Olivia E. , Coyle Carolyn , Johnson Jared , Chung Michael T. , Yoo George H. 
Published: June 2023

AbstractBackgroundAs the ongoing public health crisis from Coronavirus Disease 2019 (COVID‐19) pandemic puts strains on current models of cancer care, many health care centers had to adapt to minimize the risk of exposure and infection. The effects of the COVID‐19 pandemic in a comprehensive cancer center were determined.AimsTo measure the impact of the COVID‐19 pandemic on care delivery at a comprehensive cancer center.MethodsThe number of on‐site and telehealth visits (TH) were obtained from scheduling software. Multiple factors including total visits, telehealth visits, screenings for cancer diagnosis, and cancer treatments were tracked from 2 years before the pandemic onset through 2022. The length of stay (LOS) and Case Mix Index (CMI) were calculated using hospital database.ResultsIn the third quarter of FY 2020, telehealth visits (TH) represented a fifth of total patient encounters. Cancer treatments, such as chemotherapy, radiation therapy, and surgery, decreased during the pandemic with number of surgeries being most affected (23% decrease in 2020 compared to the previous fiscal year). The average length of stay (LOS) was also longer with less discharges per given time during the pandemic. The increased LOS was related to increased severity of patient illnesses since CMI was higher. Screening mammograms decreased to a nadir of 58% in 2021 as compared to those screened in pre‐pandemic fiscal years.ConclusionThe COVID‐19 pandemic impacted many aspects of care, such as treatment and screenings. Many of these factors had to be postponed due to the fear of acquiring COVID‐19 and access to care. The findings presented implicate that the delays and changes in cancer care during the pandemic resulted in less screening and treatment of more advanced disease.
Spatial heterogeneity of soil dissolved organic matter characteristics in the riparian zone of the Three Gorges Reservoir
Authors: Zhao Yun , Kumar Amit , Wang Kehong , Lin Junjie , Yu Zhiguo , Cheng Shuxun , Zhang Shuai , Yu Zhuolin , Liu Dan 
Published: June 2023

AbstractDissolved organic matter (DOM) is one of the active soil carbon fractions. The characteristics of DOM in the riparian zone are closely related to the occurrence of eutrophication. However, there is no scientific consensus on how flooding intensity on DOM distribution in the riparian zone of the Three Gorges Reservoir (TGR). In this study, 99 soil riparian samples were collected from three water level elevations (145–155 m asl, 155–165 m asl and 165–175 m asl) in both mainstream and tributaries of the Yangtze River within the TGR. DOM characteristics were investigated using ultraviolet–visible absorption spectra and three‐dimensional fluorescence excitation–emission matrix‐parallel factor analysis as well as principal component analysis (PCA). The result showed that dissolved organic carbon (DOC), absorption coefficient at 254 nm (a254), absorption coefficient at 350 nm, specific ultraviolet absorbance at 254 nm (SUVA254), and humification index (HIX) decreased while freshness index (β:α) and biological index (BIX) increased with the decline of the water level elevations. The trend in the DOM characteristics along the riparian zone was mainly attributed to the variation in the bioavailability of DOM and fluorescence components (C1, C3 and C4). Overall, our study highlights the newly formed DOM during the dry season, indicated by β:α and BIX, might be more accumulated at the lower elevation. Furthermore, because of the greater lability during the reservoir impoundment, the newly formed DOM can be easily utilized by algae and may raise water eutrophication under re‐flooding and longer flooding, thus enforced strategic mitigation measures could help to achieve the goal of riparian restoration in TGR.
Sex differences in lifetime prevalence of low back pain: A multinational study of opposite‐sex twin pairs
Authors: Calais‐Ferreira Lucas , Pozzobon Daniel , Pinheiro Marina B. , Blyth Fiona M. , Ordoñana Juan R. , Duncan Glen E. , Hopper John L. , Ferreira Paulo H. , Ferreira Manuela L. 
Published: June 2023

AbstractBackgroundLow back pain (LBP) is more likely to occur in people with a family history of this condition, highlighting the importance of accounting for familial factors when studying the individual risk of LBP. We conducted a study of opposite‐sex twin pairs investigating sex differences in LBP while accounting for (genetic and shared environmental) familial factors.MethodsWe applied a matched co‐twin control design to study 795 adult opposite‐sex pairs from Australia, Spain, and the United States (US). We used mixed‐effects logistic regression to assess the within‐pair association between female sex and lifetime prevalence of LBP in unadjusted and adjusted models for body‐mass‐index, and depression, as well as interactions between female sex and age (<median age vs. ≥median age) in this association.ResultsThe mean age of the sample was 47.4 years (Standard Deviation = 16.5). The adjusted odds ratio (aOR) of the association between sex and LBP in the merged sample was 1.11 (95% Confidence Interval = 0.88–1.40), with 87.4% of the variance in the studied association explained by between‐site heterogeneity (Q test; p = 0.001). Females had 2.37 (95% CI: 1.48–3.78) higher odds of LBP compared to their male co‐twins in the Spanish sample (adjusted), but a sex association was not found in the Australian nor US samples.ConclusionsWe found no evidence of the association between sex and LBP in our merged sample. Between‐population differences (i.e. cultural background or health system characteristics) are likely to be major factors leading to variation in the sex association with LBP when familial factors are accounted for.SignificanceOur study of adult opposite‐sex twin pairs found no evidence of an association between female sex and lifetime prevalence of low back pain after controlling for familial factors in the merged sample from Australia, Spain and USA, contrary to findings from previous studies of unrelated individuals. Our findings indicate potentially relevant between‐country genetic, cultural and environmental differences which may need to be considered for optimal and individualized strategies for the prevention and management of low back pain across the lifespan.