bluuebunny/crossref_metadata_2025_split huggingface dataset:
Seasonal variation in the lipid content of Fraser River Chinook Salmon (Oncorhynchus tshawytscha) and its implications for Southern Resident Killer Whale (Orcinus orca) prey quality
Authors: Lerner Jacob E. , Hunt Brian P. V. 
Published: February 2023

AbstractIn Southern British Columbia (BC), Canada, declines in southern resident killer whale (SRKW—Orcinus orca) populations have been linked to declines in numbers and average size of their preferred prey, Chinook salmon (Oncorhynchus tshawytscha). However, the life history diversity of Chinook suggests that there is a need to assess stock-specific differences in energy density to evaluate prey quality as a factor in SRKW declines. In this study, we calibrated a Distell fat meter to estimate Chinook whole-body lipid content, a proxy for energy density. The fat meter was deployed at the Fraser River, BC, Chinook test fishery during 2020, collecting lipid, weight, and length measurements from 1566 genetically stock identified individuals encompassing all major Fraser River Chinook population units (management units, MUs) at river entry. We found that MU-specific lipid content increased with distance and elevation to spawning grounds and was highest in the Spring-52 (12.8%) and Summer-52 (12.7%) MUs, intermediate in the Summer-41 MU (10.8%), and lowest in the Fall-41 MU (7.3%). Lipid content also decreased by up to 6 percentage points within MUs from the beginning to end of their migration period. Our data revealed SRKWs’ most endangered prey sources, the Spring-52 and Summer-52 MUs, are also its most energy rich. It also indicated SRKWs have access to progressively lower energy density Chinook through the year, requiring up to ~ 30% more fish to meet energy demands in the fall than in the spring.
High and low worriers do not differ in unstimulated resting-state brain connectivity
Authors: Weber-Goericke Fanny , Muehlhan Markus 
Published: February 2023

AbstractChronic, excessive and uncontrollable worry presents an anxiety rising and distressing mental activity relevant in a range of psychological disorders. Task based studies investigating its underlying neural mechanisms reveal fairly heterogenous results. The current study aimed to investigate pathological worry related effects on the functional neural network architecture in the resting unstimulated brain. Using resting-state functional magnetic resonance imaging (rsfMRI) we compared functional connectivity (FC) patterns between 21 high worriers and 21 low worriers. We, on the one hand, conducted a seed-to-voxel analysis based on recent meta-analytic findings and, on the other hand, implemented a data-driven multi voxel pattern analysis (MVPA) approach to yield brain clusters showing connectivity differences between the two groups. Additionally, the seed regions and MVPA were used to investigate whether whole brain connectivity is associated with momentary state worry across groups. The data did not reveal differences in resting-state FC related to pathological worry, neither by the seed-to-voxel or MVPA approach testing for differences linked to trait worry nor by using the MVPA to test for state worry related aberrations. We discuss whether the null findings in our analyses are related to spontaneous fluctuations in momentary worry and the associated presence of multiple fluctuating brain states that could cause mutually cancelling effects. For future studies investigating the neural correlates of excessive worry, we propose a direct worry induction for better control of the situation.
Efficient quantization of painting images by relevant colors
Authors: Tirandaz Zeinab , Foster David H. , Romero Javier , Nieves Juan Luis 
Published: February 2023

AbstractRealistic images often contain complex variations in color, which can make economical descriptions difficult. Yet human observers can readily reduce the number of colors in paintings to a small proportion they judge as relevant. These relevant colors provide a way to simplify images by effectively quantizing them. The aim here was to estimate the information captured by this process and to compare it with algorithmic estimates of the maximum information possible by colorimetric and general optimization methods. The images tested were of 20 conventionally representational paintings. Information was quantified by Shannon’s mutual information. It was found that the estimated mutual information in observers’ choices reached about 90% of the algorithmic maxima. For comparison, JPEG compression delivered somewhat less. Observers seem to be efficient at effectively quantizing colored images, an ability that may have applications in the real world.
Targeting mTOR to overcome resistance to hormone and CDK4/6 inhibitors in ER-positive breast cancer models
Authors: Rodriguez María Jimena , Perrone María Cecilia , Riggio Marina , Palafox Marta , Salinas Valeria , Elia Andrés , Salgueiro Natali Daiana , Werbach Andrea Eugenia , Marks María Paula , Kauffman Marcelo A. , Vellón Luciano , Serra Violeta , Novaro Virginia 
Published: February 2023

AbstractResistance to therapy remains a major obstacle in cancer management. Although treatment with hormone and CDK4/6 inhibitors is successful in luminal breast cancer, resistance to these treatments is frequent, highlighting the need for novel therapeutic strategies to delay disease progression and improve patient survival. Here, we assessed the mechanisms of acquired resistance using T47D and MCF-7 tamoxifen- and palbociclib-resistant cell-line variants in culture and as xenografts, and patient-derived cells (PDCs) obtained from sensitive or resistant patient-derived xenografts (PDXs). In these models, we analyzed the effect of specific kinase inhibitors on survival, signaling and cellular aggressiveness. Our results revealed that mTOR inhibition is more effective than PI3K inhibition in overcoming resistance, irrespective of PIK3CA mutation status, by decreasing cell proliferation and tumor growth, as well as reducing cell migration and stemness. Moreover, a combination of mTOR and CDK4/6 inhibitors may prevent pathway reactivation downstream of PI3K, interfering with the survival of resistant cells and consequent tumor escape. In conclusion, we highlight the benefits of incorporating mTOR inhibitors into the current therapy in ER + breast cancer. This alternative therapeutic strategy not only enhances the antitumor response but may also delay the emergence of resistance and tumor recurrence.
Low hemoglobin and PSA kinetics are prognostic factors of overall survival in metastatic castration-resistant prostate cancer patients
Authors: Hakozaki Yuji , Yamada Yuta , Takeshima Yuta , Taguchi Satoru , Kawai Taketo , Nakamura Masaki , Iwaki Takuya , Teshima Taro , Kinoshita Yoshitaka , Akiyama Yoshiyuki , Sato Yusuke , Yamada Daisuke , Suzuki Motofumi , Kume Haruki 
Published: February 2023

AbstractThe objective of this study was to identify the prognostic factors and to propose a new risk model in metastatic castration-resistant prostate cancer (mCRPC) patients. The clinical data were retrospectively obtained for 102 mCRPC patients who received cancer treatment between 2005 and 2018 at the University of Tokyo Hospital. We investigated clinical and pathological parameters, including prostate-specific antigen (PSA) kinetic profiles under androgen deprivation treatment, and identified predictors of overall survival (OS). The median age and PSA were 73 (Interquartile range [IQR], 68–79) years and 5.00 (IQR, 2.77–13.6) ng/ml. The median follow-up was 34 (IQR, 17–56) months. In univariate analysis, ‘lymph node metastasis’, ‘Hemoglobin (Hb)’, ‘Time to nadir PSA (TNPSA)’, ‘PSA doubling time (PSADT)’, ‘Time to CRPC’, and ‘presence of pain’ were prognostic factors. Multivariate analysis identified ‘Hb < 11 g/dL’, ‘TNPSA < 7 months’ and ‘PSADT < 5 months’ as independent prognostic factors of OS. The high-risk group (patients with two or three factors) demonstrated shorter OS (23 vs. 50 months) with an increased risk of death (HR = 2.997; 95% CI 1.632–5.506; P = 0.0004). The proposed risk stratification model may contribute to the prediction of survival and provide supportive information in treatment decision-making.
Intestinal epithelial barrier integrity investigated by label-free techniques in ulcerative colitis patients
Authors: Quansah Elsie , Gardey Elena , Ramoji Anuradha , Meyer-Zedler Tobias , Goehrig Bianca , Heutelbeck Astrid , Hoeppener Stephanie , Schmitt Michael , Waldner Maximillian , Stallmach Andreas , Popp Jürgen 
Published: February 2023

AbstractThe intestinal epithelial barrier, among other compartments such as the mucosal immune system, contributes to the maintenance of intestinal homeostasis. Therefore, any disturbance within the epithelial layer could lead to intestinal permeability and promote mucosal inflammation. Considering that disintegration of the intestinal epithelial barrier is a key element in the etiology of ulcerative colitis, further assessment of barrier integrity could contribute to a better understanding of the role of epithelial barrier defects in ulcerative colitis (UC), one major form of chronic inflammatory bowel disease. Herein, we employ fast, non-destructive, and label-free non-linear methods, namely coherent anti-Stokes Raman scattering (CARS), second harmonic generation (SHG), two-photon excited fluorescence (TPEF), and two-photon fluorescence lifetime imaging (2P-FLIM), to assess the morpho-chemical contributions leading to the dysfunction of the epithelial barrier. For the first time, the formation of epithelial barrier gaps was directly visualized, without sophisticated data analysis procedures, by the 3D analysis of the colonic mucosa from severely inflamed UC patients. The results were compared with histopathological and immunofluorescence images and validated using transmission electron microscopy (TEM) to indicate structural alterations of the apical junction complex as the underlying cause for the formation of the epithelial barrier gaps. Our findings suggest the potential advantage of non-linear multimodal imaging is to give precise, detailed, and direct visualization of the epithelial barrier in the gastrointestinal tract, which can be combined with a fiber probe for future endomicroscopy measurements during real-time in vivo imaging.
Molecular biological features of cyst wall of adamantinomatous craniopharyngioma
Authors: Zhao Chuan , Wang Ye , Liu Hongxing , Qi Xueling , Zhou Zhongqing , Wang Xianlong , Lin Zhixiong 
Published: February 2023

AbstractThe molecular biological differences between cyst walls and those in solid bodies are the foundation of the outcomes. In this study, the CTNNB1 mutations were confirmed by DNAsequencing; CTNNB1 expression levels were detected by PCR; the differences between solid bodies and cyst walls in proliferative capacity and tumor stem cell niches were assessed by immunohistochemistry; the effect of the residual cyst wall on recurrence was assessed by follow-up. Mutations in the CTNNB1 in the cyst wall and the solid body were identical in each case. No differences were found in the transcriptional level of CTNNB1 between the cyst walls and the solid bodies (P = 0.7619). The cyst wall showed a pathological structure similar to the solid body. Proliferative capacity of cyst walls was stronger than that of solid body (P = 0.0021), and β-catenin nuclear positive cells (cell clusters) in cyst walls were more than that in solid tumor (P = 0.0002). The retrospective 45 ACPs showed residual cyst wall was significantly associated with tumor recurrence or regrowth (P = 0.0176). Kaplan–Meier analysis showed there was a significant difference in the prognosis between GTR and STR (P < 0.0001).The cyst wall of ACP contained more tumor stem cell niches which could lead to the recurrence. According to the above-mentioned, a special attention to the management of the cyst wall should be paid.
Extracellular vesicles released from macrophages modulates interleukin-1β in astrocytic and neuronal cells
Authors: Kodidela Sunitha , Sinha Namita , Kumar Asit , Zhou Lina , Godse Sandip , Kumar Santosh 
Published: February 2023

AbstractWe have recently demonstrated that long-term exposure of cigarette smoke condensate (CSC) to HIV-uninfected (U937) and -infected (U1) macrophages induce packaging of pro-inflammatory molecules, particularly IL-1β, in extracellular vesicles (EVs). Therefore, we hypothesize that exposure of EVs derived from CSC-treated macrophages to CNS cells can increase their IL-1β levels contributing to neuroinflammation. To test this hypothesis, we treated the U937 and U1 differentiated macrophages once daily with CSC (10 µg/ml) for 7 days. Then, we isolated EVs from these macrophages and treated these EVs with human astrocytic (SVGA) and neuronal (SH-SY5Y) cells in the absence and presence of CSC. We then examined the protein expression of IL-1β and oxidative stress related proteins, cytochrome P450 2A6 (CYP2A6), superoxide dismutase-1 (SOD1), catalase (CAT). We observed that the U937 cells have lower expression of IL-1β compared to their respective EVs, confirming that most of the produced IL-1β are packaged into EVs. Further, EVs isolated from HIV-infected and uninfected cells, both in the absence and presence of CSC, were treated to SVGA and SH-SY5Y cells. These treatments showed a significant increase in the levels of IL-1β in both SVGA and SH-SY5Y cells. However, under the same conditions, the levels of CYP2A6, SOD1, and catalase were only markedly altered. These findings suggest that the macrophages communicate with astrocytes and neuronal cells via EVs-containing IL-1β in both HIV and non-HIV setting and could contribute to neuroinflammation.
Dose–response of benazepril on biomarkers of the classical and alternative pathways of the renin–angiotensin–aldosterone system in dogs
Authors: Sotillo Samantha , Ward Jessica L. , Guillot Emilie , Domenig Oliver , Yuan Lingnan , Smith Joseph S. , Gabriel Vojtech , Iennarella-Servantez Chelsea A. , Mochel Jonathan P. 
Published: February 2023

AbstractAngiotensin-converting enzyme inhibitors (ACEI) such as benazepril are commonly prescribed in both humans and dogs with heart disease to mitigate the renin–angiotensin–aldosterone system (RAAS); however, the dose-dependent effects of benazepril on comprehensive RAAS components remain unknown. In this study, nine purpose-bred healthy dogs received three different dosages of oral benazepril (0.125 mg/kg, 0.25 mg/kg, or 0.5 mg/kg) in a randomized crossover design following induction of RAAS activation by consuming a low-sodium diet. Blood samples were collected at serial time intervals after benazepril dosing to measure plasma benazeprilat (active metabolite of benazepril) and serum RAAS biomarkers. Blood pressure and echocardiogram were performed at baseline and after each benazepril administration. Time-weighted averages for RAAS biomarkers for 12 h post-dose and hemodynamic variables were compared between dosing groups using Wilcoxon rank-sum testing. Compared to the lowest dosage of benazepril (0.125 mg/kg), the highest dosage (0.5 mg/kg) resulted in lower time-weighted average values of angiotensin (Ang) II (− 38%, P = 0.004), Ang1-5 (− 53%, P = 0.001), ACE-S (surrogate for ACE activity; − 59%, P = 0.0002), and ALT-S (surrogate for alternative RAAS activity; − 22%, P = 0.004), and higher values of AngI (+ 78%, P = 0.014) and PRA-S (surrogate for plasma renin activity; + 58%, P = 0.040). There were no relevant differences between dosing groups for blood pressure or echocardiographic variables. Knowledge of dose-dependent alterations in biomarkers of the classical and alternative RAAS pathways could help inform clinical trials for dosage optimization in both dogs and humans.
Effects of saccade delay, side of deficit, and training on detection of catch-up saccades during head-impulse test in virtual-reality-enhanced mannequin
Authors: Charlery-Adèle Ambre , Guigou Caroline , Ryard Julien , Chartier Mathis , Toupet Michel , Guillet Christophe , Mérienne Férédric , Bozorg Grayeli Alexis 
Published: February 2023

AbstractIn this study, a training simulator for the examination of dizzy patients based on a virtual-reality-enhanced mannequin (VREM) was developed to evaluate the detection of catch-up saccades during head impulse test (HIT) and the effect of training in VREM. For novices (n = 35), 2 trials were conducted before and after a training session. Experts (n = 7) were submitted to an evaluation session. In each trial, a left or a right horizontal canal deficit with an overt catch-up saccade (delay between 110 and 320 ms) was randomly presented. Participants scored the difficulty in performing the maneuver, in recognizing the saccades, and the self-confidence in the diagnosis using a visual analogue scale (VAS). Saccade delay significantly influenced the performance. Training significantly improved the sensitivity in the residents (69.1% before to 97.9% after the training, p < 0.001, Fisher's exact test, n = 560 tests), surpassing experts’ performances (p < 0.001, versus 87% in experts, Fisher's exact test). The specificity also increased to the expert level (78% before to 95% after the training, and 95% in experts, p < 0.001, Fisher’s exact test). The VAS showed a decrease difficulty to execute the HIT, with an increase in the confidence after training. VREM improved the HIT execution performance and the confidence in novice practitioners.
Non-synonymous variation and protein structure of candidate genes associated with selection in farm and wild populations of turbot (Scophthalmus maximus)
Authors: Andersen Øivind , Rubiolo Juan Andrés , Pirolli Davide , Aramburu Oscar , Pampín Marina , Righino Benedetta , Robledo Diego , Bouza Carmen , De Rosa Maria Cristina , Martínez Paulino 
Published: February 2023

AbstractNon-synonymous variation (NSV) of protein coding genes represents raw material for selection to improve adaptation to the diverse environmental scenarios in wild and livestock populations. Many aquatic species face variations in temperature, salinity and biological factors throughout their distribution range that is reflected by the presence of allelic clines or local adaptation. The turbot (Scophthalmus maximus) is a flatfish of great commercial value with a flourishing aquaculture which has promoted the development of genomic resources. In this study, we developed the first atlas of NSVs in the turbot genome by resequencing 10 individuals from Northeast Atlantic Ocean. More than 50,000 NSVs where detected in the ~ 21,500 coding genes of the turbot genome, and we selected 18 NSVs to be genotyped using a single Mass ARRAY multiplex on 13 wild populations and three turbot farms. We detected signals of divergent selection on several genes related to growth, circadian rhythms, osmoregulation and oxygen binding in the different scenarios evaluated. Furthermore, we explored the impact of NSVs identified on the 3D structure and functional relationship of the correspondent proteins. In summary, our study provides a strategy to identify NSVs in species with consistently annotated and assembled genomes to ascertain their role in adaptation.
Simulation and modelling of heavy metals and water quality parameters in the river
Authors: Khalilzadeh Poshtegal Maryam , Mirbagheri Seyed Ahmad 
Published: February 2023

AbstractA one-dimensional qualitative model was created for this study’s modelling and phase transfer of the heavy metal dissolved in the river. The advection–diffusion equation considers the environmental variables temperature, dissolved oxygen, pH, and electrical conductivity as influencing parameters on the change in the concentration of the dissolved phase of heavy metals lead, cadmium, and zinc in springtime and winter. Hec-Ras hydrodynamic model and Qual2kw qualitative model have been used to determine the hydrodynamic and environmental parameters in the created model. The approach of minimizing simulation errors and Vba coding was employed to identify the constant coefficients for these relations, and the linear relation incorporating all of the parameters is thought to be the final connection. In order to simulate and calculate the concentration of the heavy metals in the dissolved phase at each site, the kinetic coefficient of the reaction corresponding to that point should be employed because the kinetic coefficient of the reaction varies in different portions of the river. Additionally, if the above mentioned environmental parameters are used in the spring and winter term of advection–diffusion equations, the accuracy of the developed model significantly improves, and the effect of other qualitative parameters is negligible, indicating that the model is effective at simulating the dissolved phase of heavy metals in the river.
Effect of membrane depolarization against Aspergillus niger GM31 resistant by ultra nanoclusters characterized by Ag2+ and Ag3+ oxidation state
Authors: Molina Hernandez Junior Bernardo , Scotti Luca , Valbonetti Luca , Gioia Luisa , Paparella Antonello , Paludi Domenico , Aceto Antonio , Ciriolo Maria Rosa , Chaves Lopez Clemencia 
Published: February 2023

AbstractTo date, the impossibility of treating resistant forms of bacteria and fungi (AMR) with traditional drugs is a cause for global alarm. We have made the green synthesis of Argirium silver ultra nanoclusters (Argirium-SUNCs) very effective against resistant bacteria (< 1 ppm) and mature biofilm (0.6 ppm). In vitro and preclinical tests indicate that SUNCs are approximately 10 times less toxic in human cells than bacteria. Unique chemical-physical characteristics such as particle size < 2 nm, a core composed of Ag0, and a shell of Ag +, Ag2+ , Ag3+ never observed before in stable form in ultra pure water, explain their remarkable redox properties Otto Cars (Lancet Glob. Health 9:6, 2021). Here we show that Argirium-SUNCs have strong antimicrobial properties also against resistant Aspergillus niger GM31 mycelia and spore inactivation (0.6 ppm). The membrane depolarization is a primary target leading to cell death as already observed in bacteria. Being effective against both bacteria and fungi Argirium-SUNCs represent a completely different tool for the treatment of infectious diseases.
SPLIT-PIN software enabling confocal and super-resolution imaging with a virtually closed pinhole
Authors: Di Franco Elisabetta , Costantino Angelita , Cerutti Elena , D’Amico Morgana , Privitera Anna P. , Bianchini Paolo , Vicidomini Giuseppe , Gulisano Massimo , Diaspro Alberto , Lanzanò Luca 
Published: February 2023

AbstractIn point-scanning microscopy, optical sectioning is achieved using a small aperture placed in front of the detector, i.e. the detection pinhole, which rejects the out-of-focus background. The maximum level of optical sectioning is theoretically obtained for the minimum size of the pinhole aperture, but this is normally prevented by the dramatic reduction of the detected signal when the pinhole is closed, leading to a compromise between axial resolution and signal-to-noise ratio. We have recently demonstrated that, instead of closing the pinhole, one can reach a similar level of optical sectioning by tuning the pinhole size in a confocal microscope and by analyzing the resulting image series. The method, consisting in the application of the separation of photons by lifetime tuning (SPLIT) algorithm to series of images acquired with tunable pinhole size, is called SPLIT-pinhole (SPLIT-PIN). Here, we share and describe a SPLIT-PIN software for the processing of series of images acquired at tunable pinhole size, which generates images with reduced out-of-focus background. The software can be used on series of at least two images acquired on available commercial microscopes equipped with a tunable pinhole, including confocal and stimulated emission depletion (STED) microscopes. We demonstrate applicability on different types of imaging modalities: (1) confocal imaging of DNA in a non-adherent cell line; (2) removal of out-of-focus background in super-resolved STED microscopy; (3) imaging of live intestinal organoids stained with a membrane dye.