bluuebunny/crossref_metadata_2025_split huggingface dataset:
Reactogenicity and Peak Anti-RBD-S1 IgG Concentrations in Individuals with No Prior COVID-19 Infection Vaccinated with Different SARS-CoV-2 Vaccines
Authors: Milevoj Kopcinovic Lara , Unic Adriana , Nikolac Gabaj Nora , Miler Marijana , Vrtaric Alen , Bozovic Marija , Stefanovic Mario 
Published: June 2023

Abstract

Objective
To investigate the association of immune response with vaccination adverse effects at peak anti–receptor-binding domain spike subunit 1 (anti-RBDS1) IgG after full vaccination with Comirnaty, Spikevax, or Vaxzevria.

Methods
Anti-RBDS1 IgG concentrations after vaccination were determined in healthy adults vaccinated with the Comirnaty, Spikevax, and Vaxzevria vaccines. The association of reactogenicity and peak antibody response after vaccination was tested.

Results
Anti-RBDS1 IgG values were significantly higher in the Comirnaty and Spikevax group, compared with the Vaxzevria group (P &lt; .001). Fever and muscle pain were found to be significant independent predictors of peak anti-RBDS1 IgG in the Comirnaty and Spikevax groups (P = .03 and P = .02, respectively). The multivariate model, adjusted for covariates, showed that no association between reactogenicity and peak antibody concentrations was found in the Comirnaty, Spikevax, and Vaxzevria groups.

Conclusions
No association between reactogenicity and peak anti-RBDS1 IgG after vaccination with the Comirnaty, Spikevax, and Vaxzevria vaccine was found.
A Market Assessment of Introducer Technology to Aid With Endotracheal Intubation
Authors: Gutierrez Gianna A , Henry Jevaughn , April Michael D , Long Brit J , Schauer Steven G 
Published: June 2023

ABSTRACT

Introduction
Endotracheal intubation is a potentially lifesaving procedure. Previously, data demonstrated that intubation remains the most performed airway intervention in the Role 1 setting. Additionally, deployed data demonstrate that casualties intubated in the prehospital setting have worse survival than those intubated in the emergency department setting. Technological solutions may improve intubation success in this setting. Certain intubation practices, including the use of endotracheal tube introducer bougies, facilitate intubation success especially in patients with difficult airways. We sought to determine the current state of the market for introducer devices.

Materials and Methods
This market review utilized Google searches to find products for intubation. The search criteria aimed to identify any device ideal for intubation in the emergency setting. Device data retrieved included manufacturer, device, cost, and design descriptions.

Results
We identified 12 introducer-variants on the market. Devices varied with regards to composition (latex, silicone, polyethylene, combination of several materials, etc.), tip shape, special features for ease of intubation (markings for depth and visibility, size, etc.), disposability/reuse capability, measurements, and prices. The cost of each device ranged from approximately $5 to $100.

Conclusions
We identified 12 introducer-variants on the market. Clinical studies are necessary to determine which devices may improve patient outcomes in the Role 1 setting.
Cannabis Use Among U.S. Military Veterans Following Residential Substance Use Disorder Treatment
Authors: Mian Maha N , Chan Osilla Karen , Blonigen Daniel 
Published: June 2023

ABSTRACT

Introduction
Use rates of cannabis, a substance associated with a host of comorbid physical and mental health concerns, continue to rise for military veterans. Despite this prevalence, descriptive patterns of use among veterans and research on treatment factors that predict cannabis outcomes are lacking. This study aimed to conduct a descriptive profile of veterans who endorse cannabis use, compare veterans endorsing cannabis use to those who do not endorse use, and investigate what factors (other substance use, psychiatric symptoms, and treatment outcomes) predicted return to cannabis use following residential treatment.

Materials and Methods
The study was a secondary data analysis of a longitudinal sample of U.S. military veterans (N = 200, 193 males, Mage = 50.14, SD = 9) participating in residential substance use disorder treatment through a Veterans Affairs medical center. Interview, survey, and electronic health data were collected over 12 months. Analyses included descriptive and frequency statistics to identify patterns in cannabis use behaviors and motives, independent t-tests to examine differences between the cannabis-using group and non-using group, and a series of univariate logistic regressions to examine potential predictors for cannabis use after treatment discharge.

Results
Lifetime cannabis use was common among veterans (77.5%), and 29.5% reported use during the study. On average, veterans had made one quit attempt before treatment entry. Veterans who endorsed cannabis use consumed more alcohol in the past 30 days at baseline and reported less impulse control and less confidence in maintaining abstinence at discharge. Length of stay in the residential program and no diagnosis of Diagnostic and Statistical Manual of Mental Disorders (DSM)-IV cannabis use disorder criteria predicted post-treatment cannabis use such that veterans who remained in the program longer were more likely to abstain from cannabis use following treatment, and those who did not meet DSM-IV cannabis use disorder criteria were more likely to use following treatment.

Conclusions
Identification of relevant risk factors and treatment processes, such as impulse control, confidence in treatment, and length of stay in treatment, provides practical recommendations for future intervention efforts. This study calls for further examination of cannabis use outcomes among veterans, particularly those participating in substance use treatment.
Rapid radio brightening of GRB 210702A
Authors: Anderson G E , Russell T D , Fausey H M , van der Horst A J , Hancock P J , Bahramian A , Bell M E , Miller-Jones J C A , Rowell G , Sammons M W , Wijers R A M J , Galvin T J , Goodwin A J , Konno R , Rowlinson A , Ryder S D , Schüssler F , Wagner S J , Zhu S J 
Published: June 2023

ABSTRACT
We observed the rapid radio brightening of GRB 210702A with the Australia Telescope Compact Array (ATCA) just 11 h post-burst, tracking early-time radio variability over a 5 h period on ∼15 min time-scales at 9.0, 16.7, and 21.2 GHz. A broken power law fit to the 9.0 GHz light curve showed that the 5 h flare peaked at a flux density of 0.4 ± 0.1 mJy at ∼13 h post-burst. The observed temporal and spectral evolution is not expected in the standard internal–external shock model, where forward and reverse shock radio emission evolves on much longer time-scales. The early-time (&lt;1 d) optical and X-ray light curves from the Neil Gehrels Swift Observatory demonstrated typical afterglow forward shock behaviour, allowing us to use blast wave physics to determine a likely homogeneous circumburst medium and an emitting electron population power-law index of p = 2.9 ± 0.1. We suggest that the early-time radio flare is likely due to weak interstellar scintillation (ISS), which boosted the radio afterglow emission above the ATCA sensitivity limit on minute time-scales. Using relations for ISS in the weak regime, we were able to place an upper limit on the size of the blast wave of ≲6 × 1016 cm in the plane of the sky, which is consistent with the theoretical forward shock size prediction of 8 × 1016 cm for GRB 210702A at ∼13 h post-burst. This represents the earliest ISS size constraint on a gamma-ray burst (GRB) blast wave to date, demonstrating the importance of rapid (&lt;1 d) radio follow-up of GRBs using several-hour integrations to capture the early afterglow evolution and to track the scintillation over a broad frequency range.
Properties of gamma-ray bursts associated with supernovae and kilonovae
Authors: Li Q M , Zhang Z B , Han X L , Zhang K J , Xia X L , Hao C T 
Published: June 2023

ABSTRACT
We systematically compare the temporal and spectral properties of 53 supernova (SN)-associated and 15 kilonova (KN)-associated gamma-ray bursts (GRBs). We find that the spectral parameters of both types of GRBs are identically and lognormally distributed, consistent with those normal GRBs. The bolometric luminosities of SN/GRBs and KN/GRBs have a triple form, with the corresponding break luminosities of SN/GRBs are roughly two orders of magnitude larger than those of KN/GRBs. We build the power-law relations between the spectral lag and the luminosity of prompt γ-rays with indices of −1.43 ± 0.33 for SN/GRBs and −2.17 ± 0.57 for KN/GRBs in the laboratory frame, which are respectively coincident with the rest-frame values. We verify that both SN/GRBs and KN/GRBs comply with their own Amati relations that match those of long and short GRBs, respectively. Analysing X-ray afterglows with good plateau segments, we build the power-law relations between the X-ray luminosity and the plateau time with an index of −1.12 ± 0.17 for KN/GRBs and −1.08 ± 0.22 for SN/GRBs, which can be well explained by the relativistic shock driven by an energy injection. The plots of luminosity-lag, Amati relation, and luminosity-time show heavy overlap between the two types of GRBs, implying that they might share the same radiation mechanism despite originating from different progenitors or central engines.
ERGO-ML: towards a robust machine learning model for inferring the fraction of accreted stars in galaxies from integral-field spectroscopic maps
Authors: Angeloudi Eirini , Falcón-Barroso Jesús , Huertas-Company Marc , Sarmiento Regina , Pillepich Annalisa , Walo-Martín Daniel , Eisert Lukas 
Published: June 2023

ABSTRACT
Quantifying the contribution of mergers to the stellar mass of galaxies is key for constraining the mechanisms of galaxy assembly across cosmic time. However, the mapping between observable galaxy properties and merger histories is not trivial: cosmological galaxy simulations are the only tools we have for calibration. We study the robustness of a simulation-based inference of the ex situ stellar mass fraction of nearby galaxies to different observables – integrated and spatially resolved – and to different galaxy formation models – IllustrisTNG and EAGLE – with machine learning. We find that at fixed simulation, the fraction of accreted stars can be inferred with very high accuracy, with an error ∼5 per cent (10 per cent) from 2D integral-field spectroscopic maps (integrated quantities) throughout the considered stellar mass range. A bias (&gt; 5 per cent) and an increase in scatter by a factor of 2 are introduced when testing with a different simulation, revealing a lack of generalization to distinct galaxy-formation models. Interestingly, upon using only stellar mass and kinematics maps in the central galactic regions for training, we find that this bias is removed and the ex situ stellar mass fraction can be recovered in both simulations with &lt;15 per cent scatter, independently of the training set’s origin. This opens up the door to a potential robust inference of the accretion histories of galaxies from existing integral field unit surveys, such as MaNGA, covering a similar field of view and containing spatially resolved spectra for tens of thousands of nearby galaxies.
Fixed Allele Differences Associated With the Centromere Reveal Chromosome Morphology and Rearrangements in a Reptile (Varanus acanthurus BOULENGER)
Authors: Dobry Jason , Zhu Zexian , Zhou Qi , Wapstra Erik , Deakin Janine E , Ezaz Tariq 
Published: June 2023

Abstract
Chromosome rearrangements are often implicated with genomic divergence and are proposed to be associated with species evolution. Rearrangements alter the genomic structure and interfere with homologous recombination by isolating a portion of the genome. Integration of multiplatform next-generation DNA sequencing technologies has enabled putative identification of chromosome rearrangements in many taxa; however, integrating these data sets with cytogenetics is still uncommon beyond model genetic organisms. Therefore, to achieve the ultimate goal for the genomic classification of eukaryotic organisms, physical chromosome mapping remains critical. The ridge-tailed goannas (Varanus acanthurus BOULENGER) are a group of dwarf monitor lizards comprised of several species found throughout northern Australia. These lizards exhibit extreme divergence at both the genic and chromosomal levels. The chromosome polymorphisms are widespread extending across much of their distribution, raising the question if these polymorphisms are homologous within the V. acanthurus complex. We used a combined genomic and cytogenetic approach to test for homology across divergent populations with morphologically similar chromosome rearrangements. We showed that more than one chromosome pair was involved with the widespread rearrangements. This finding provides evidence to support de novo chromosome rearrangements have occurred within populations. These chromosome rearrangements are characterized by fixed allele differences originating in the vicinity of the centromeric region. We then compared this region with several other assembled genomes of reptiles, chicken, and the platypus. We demonstrated that the synteny of genes in Reptilia remains conserved despite centromere repositioning across these taxa.
A Novel Group of Dynamin-Related Proteins Shared by Eukaryotes and Giant Viruses Is Able to Remodel Mitochondria From Within the Matrix
Authors: Sheikh Shaghayegh , Pánek Tomáš , Gahura Ondřej , Týč Jiří , Záhonová Kristína , Lukeš Julius , Eliáš Marek , Hashimi Hassan 
Published: June 2023

Abstract
The diverse GTPases of the dynamin superfamily play various roles in the cell, as exemplified by the dynamin-related proteins (DRPs) Mgm1 and Opa1, which remodel the mitochondrial inner membrane in fungi and metazoans, respectively. Via an exhaustive search of genomic and metagenomic databases, we found previously unknown DRP types occurring in diverse eukaryotes and giant viruses (phylum Nucleocytoviricota). One novel DRP clade, termed MidX, combined hitherto uncharacterized proteins from giant viruses and six distantly related eukaryote taxa (Stramenopiles, Telonemia, Picozoa, Amoebozoa, Apusomonadida, and Choanoflagellata). MidX stood out because it was not only predicted to be mitochondria-targeted but also to assume a tertiary structure not observed in other DRPs before. To understand how MidX affects mitochondria, we exogenously expressed MidX from Hyperionvirus in the kinetoplastid Trypanosoma brucei, which lacks Mgm1 or Opa1 orthologs. MidX massively affected mitochondrial morphology from inside the matrix, where it closely associates with the inner membrane. This unprecedented mode of action contrasts to those of Mgm1 and Opa1, which mediate inner membrane remodeling in the intermembrane space. We speculate that MidX was acquired in Nucleocytoviricota evolution by horizontal gene transfer from eukaryotes and is used by giant viruses to remodel host mitochondria during infection. MidX's unique structure may be an adaptation for reshaping mitochondria from the inside. Finally, Mgm1 forms a sister group to MidX and not Opa1 in our phylogenetic analysis, throwing into question the long-presumed homology of these DRPs with similar roles in sister lineages.
Evaluation of Ministry of Health, Labour and Welfare diagnostic criteria for antineutrophil cytoplasmic antibody–associated vasculitis compared to ACR/EULAR 2022 classification criteria
Authors: Sada Ken-ei , Nagasaka Kenji , Kaname Shinya , Higuchi Tomoaki , Furuta Shunsuke , Nanki Toshihiro , Tsuboi Naotake , Amano Koichi , Dobashi Hiroaki , Hiromura Keiju , Bando Masashi , Wada Takashi , Arimura Yoshihiro , Makino Hirofumi , Harigai Masayoshi 
Published: June 2023

ABSTRACT

Objective
This study aimed to evaluate the Ministry of Health, Labour and Welfare (MHLW) diagnostic criteria for antineutrophil cytoplasmic antibody–associated vasculitis compared to the new American College of Rheumatology/European Alliance of Associations for Rheumatology 2022 criteria.

Methods
Two nationwide cohort studies were used, and participants were categorised as having eosinophilic granulomatosis with polyangiitis, granulomatosis with polyangiitis (GPA), or microscopic polyangiitis (MPA) according to the American College of Rheumatology/European Alliance of Associations for Rheumatology 2022 and MHLW criteria.

Results
Of the entire patient population, only 10 (2.1%) were unclassifiable according to the MHLW probable criteria, while a significant number of patients (71.3%) met at least two criteria. The MHLW probable criteria for MPA had some challenges in differentiating between MPA and eosinophilic granulomatosis with polyangiitis, and the same was true for MHLW probable criteria for GPA in differentiating MPA from GPA. Nevertheless, improved classification results were obtained when the MHLW probable criteria were applied in the order of eosinophilic granulomatosis with polyangiitis, MPA, and GPA.

Conclusions
The application of MHLW criteria could categorise a substantial number of patients with antineutrophil cytoplasmic antibody–associated vasculitis into one of the three antineutrophil cytoplasmic antibody–associated vasculitis diseases. The classification was in accordance with the American College of Rheumatology/European Alliance of Associations for Rheumatology 2022 criteria when considering the order of application.
Long-term patency of aorto-biiliac endoprosthesis for critical lower limb ischaemia in Takayasu arteritis after complicated angioplasty with a drug-coated balloon: Effect of dual antiplatelet therapy combined with tocilizumab
Authors: Leal Thatiany Paslar , Pinto Melissa Pereira Lopes Vieira , Hasselmann Gabriela , Lammoglia Bruna Cremonezi , Trevise Luana de Aguiar , Salles Rosa Neto Nilton 
Published: June 2023

ABSTRACT
Takayasu arteritis is a chronic granulomatous vasculitis of unknown aetiology affecting the aorta and its major branches. Critical limb ischaemia may occur and eventually require surgical intervention. Surgical outcomes are influenced by disease activity, age, and comorbidities. We report a 43-year-old woman with Takayasu arteritis and stenosis of the left common iliac artery and occlusion of the left external iliac artery with limiting vascular claudication, who underwent angioplasty of the iliac artery with drug-eluting stent while being treated with infliximab. The artery ruptured a week later but was contained by the ilio-psoas muscle. She required subsequent stent placement to correct the lesion. Treatment comprised aspirin and clopidogrel, and the biological was switched to monthly intravenous tocilizumab. During an 8-year follow-up, serial imaging examinations showed a patent aorto-biiliac endoprosthesis, without evidence of thrombosis or restenosis. Clinically, the patient denies vascular claudication and pulses remain palpable in the left lower limb. This case highlights the risks inherent to these procedures in patients with large artery vasculitis and reinforces that the effectiveness of endovascular intervention can be increased by detailed preoperative evaluation, associated with a drug strategy including immunomodulatory and antiplatelet therapy as directed by the multispecialty team. Periodic imaging examinations are required because of the reported high rate of restenosis.
An amber-encoding helper phage for more efficient phage display of noncanonical amino acids
Authors: Hampton Joshua Trae , Cho Chia-Chuan Dean , Coleman Demonta D , Geng Zhi Zachary , Chen Peng-Hsun Chase , Dubey Gopal K , Sylvain Lauralee D , Xu Shiqing , Liu Wenshe Ray 
Published: June 2023

Abstract
Using an amber suppression-based noncanonical amino acid (ncAA) mutagenesis approach, the chemical space in phage display can be significantly expanded for drug discovery. In this work, we demonstrate the development of a novel helper phage, CMa13ile40, for continuous enrichment of amber obligate phage clones and efficient production of ncAA-containing phages. CMa13ile40 was constructed by insertion of a Candidatus Methanomethylophilus alvus pyrrolysyl-tRNA synthetase/PylT gene cassette into a helper phage genome. The novel helper phage allowed for a continuous amber codon enrichment strategy for two different libraries and demonstrated a 100-fold increase in packaging selectivity. CMa13ile40 was then used to create two peptide libraries containing separate ncAAs, Nϵ-tert-butoxycarbonyl-lysine and Nϵ-allyloxycarbonyl-lysine, respectively. These libraries were used to identify peptide ligands that bind to the extracellular domain of ZNRF3. Each selection showed differential enrichment of unique sequences dependent upon the ncAA used. Peptides from both selections were confirmed to have low micromolar affinity for ZNRF3 that was dependent upon the presence of the ncAA used for selection. Our results demonstrate that ncAAs in phages provide unique interactions for identification of unique peptides. As an effective tool for phage display, we believe that CMa13ile40 can be broadly applied to a wide variety of applications.
Extension of mRNA poly(A) tails and 3′UTRs during neuronal differentiation exhibits variable association with post-transcriptional dynamics
Authors: Kiltschewskij Dylan J , Harrison Paul F , Fitzsimmons Chantel , Beilharz Traude H , Cairns Murray J 
Published: June 2023

Abstract
Differentiation of neural progenitor cells into mature neuronal phenotypes relies on extensive temporospatial coordination of mRNA expression to support the development of functional brain circuitry. Cleavage and polyadenylation of mRNA has tremendous regulatory capacity through the alteration of mRNA stability and modulation of microRNA (miRNA) function, however the extent of utilization in neuronal development is currently unclear. Here, we employed poly(A) tail sequencing, mRNA sequencing, ribosome profiling and small RNA sequencing to explore the functional relationship between mRNA abundance, translation, poly(A) tail length, alternative polyadenylation (APA) and miRNA expression in an in vitro model of neuronal differentiation. Differential analysis revealed a strong bias towards poly(A) tail and 3′UTR lengthening during differentiation, both of which were positively correlated with changes in mRNA abundance, but not translation. Globally, changes in miRNA expression were predominantly associated with mRNA abundance and translation, however several miRNA–mRNA pairings with potential to regulate poly(A) tail length were identified. Furthermore, 3′UTR lengthening was observed to significantly increase the inclusion of non-conserved miRNA binding sites, potentially enhancing the regulatory capacity of these molecules in mature neuronal cells. Together, our findings suggest poly(A) tail length and APA function as part of a rich post-transcriptional regulatory matrix during neuronal differentiation.