bluuebunny/crossref_metadata_2025_split huggingface dataset:
Axisymmetric Peeling of Thin Elastic Films: A Perturbation Solution
Authors: Chen Erteng , Dai Zhaohe 
Published: June 2023

Abstract
We study the mechanical behavior of a thin elastic film that is affixed to a rigid substrate and subjected to a transverse force using a shaft with a finite radius. This scenario, also referred to as axisymmetric peeling, is encountered frequently in conventional blister tests as well as in our daily lives when removing an adhesive film from a substrate. Our primary objective is to gain a quantitative understanding of how the shaft’s radius influences the relationships between force and displacement, as well as between force and delamination areas. These relationships can serve as a dependable method to determine both the film’s elastic modulus and the adhesion strength between the film and its substrate. In this work, we provide a simple perturbation solution to this geometrically nonlinear problem while avoiding any use of ad hoc assumptions that were previously required. As a result, our results are in excellent agreement with numerical simulations and offer improved accuracy compared to analytical solutions available in the literature.
The enigmatic nucleus of the marine dinoflagellate
Prorocentrum cordatum
Authors: Kalvelage Jana , Wöhlbrand Lars , Schoon Robin-Alexander , Zink Fiona-Marine , Correll Christina , Senkler Jennifer , Eubel Holger , Hoppenrath Mona , Rhiel Erhard , Braun Hans-Peter , Winklhofer Michael , Klingl Andreas , Rabus Ralf 
Published: June 2023

ABSTRACT

The marine, bloom-forming dinoflagellate
Prorocentrum cordatum
CCMP 1329 (formerly
P. minimum
) has a genome atypical of eukaryotes, with a large size of ~4.15 Gbp, organized in plentiful, highly condensed chromosomes and packed in a dinoflagellate-specific nucleus (dinokaryon). Here, we apply microscopic and proteogenomic approaches to obtain new insights into this enigmatic nucleus of axenic
P. cordatum
. High-resolution focused ion beam/scanning electron microscopy analysis of the flattened nucleus revealed highest density of nuclear pores in the vicinity of the nucleolus, a total of 62 tightly packed chromosomes (~0.4–6.7 µm
3
), and interaction of several chromosomes with the nucleolus and other nuclear structures. A specific procedure for enriching intact nuclei was developed to enable proteomic analyses of soluble and membrane protein-enriched fractions. These were analyzed with geLC and shotgun approaches employing ion-trap and timsTOF (trapped-ion-mobility-spectrometry time-of-flight) mass spectrometers, respectively. This allowed identification of 4,052 proteins (39% of unknown function), out of which 418 were predicted to serve specific nuclear functions; additional 531 proteins of unknown function could be allocated to the nucleus. Compaction of DNA despite very low histone abundance could be accomplished by highly abundant major basic nuclear proteins (HCc2-like). Several nuclear processes including DNA replication/repair and RNA processing/splicing can be fairly well explained on the proteogenomic level. By contrast, transcription and composition of the nuclear pore complex remain largely elusive. One may speculate that the large group of potential nuclear proteins with currently unknown functions may serve yet to be explored functions in nuclear processes differing from those of typical eukaryotic cells.

IMPORTANCE

Dinoflagellates form a highly diverse group of unicellular microalgae. They provide keystone species for the marine ecosystem and stand out among others by their very large, unusually organized genomes embedded in the nuclei markedly different from other eukaryotic cells. Functional insights into nuclear and other cell biological structures and processes of dinoflagellates have long been hampered by the paucity of available genomic sequences. The here studied cosmopolitan
P. cordatum
belongs to the harmful algal bloom-forming, marine dinoflagellates and has a recently
de novo
assembled genome. We present a detailed 3D reconstruction of the
P. cordatum
nucleus together with comprehensive proteogenomic insights into the protein equipment mastering the broad spectrum of nuclear processes. This study significantly advances our understanding of mechanisms and evolution of the conspicuous dinoflagellate cell biology.
Sodium butyrate facilitates CRHR2 expression to alleviate HPA axis hyperactivity in autism-like rats induced by prenatal lipopolysaccharides through histone deacetylase inhibition
Authors: Wang Xinyuan , Sun Zhujun , Yang Ting , Lin Fang , Ye Shasha , Yan Junyan , Li Tingyu , Chen Jie 
Published: June 2023

ABSTRACT

Short-chain fatty acids (SCFAs, especially butyric acid) have been demonstrated to play a promising role in the development of autism spectrum disorders (ASD). Recently, the hypothalamic–pituitary–adrenal (HPA) axis is also suggested to increase the risk of ASD. However, the mechanism underlying SCFAs and HPA axis in ASD development remains unknown. Here, we show that children with ASD exhibited lower SCFA concentrations and higher cortisol levels, which were recaptured in prenatal lipopolysaccharide (LPS)-exposed rat model of ASD. These offspring also showed decreased SCFA-producing bacteria and histone acetylation activity as well as impaired corticotropin-releasing hormone receptor 2 (CRHR2) expression. Sodium butyrate (NaB), which can act as histone deacetylases inhibitors, significantly increased histone acetylation at the CRHR2 promoter
in vitro
and normalized the corticosterone as well as CRHR2 expression level
in vivo
. Behavioral assays indicated ameliorative effects of NaB on anxiety and social deficit in LPS-exposed offspring. Our results imply that NaB treatment can improve ASD-like symptoms via epigenetic regulation of the HPA axis in offspring; thus, it may provide new insight into the SCFA treatment of neurodevelopmental disorders like ASD.

IMPORTANCE

Growing evidence suggests that microbiota can affect brain function and behavior through the “microbiome–gut–brain’’ axis, but its mechanism remains poorly understood. Here, we show that both children with autism and LPS-exposed rat model of autism exhibited lower SCFA concentrations and overactivation of HPA axis. SCFA-producing bacteria,
Lactobacillus
, might be the key differential microbiota between the control and LPS-exposed offspring. Interestingly, NaB treatment contributed to the regulation of HPA axis (such as corticosterone as well as CRHR2) and improvement of anxiety and social deficit behaviors in LPS-exposed offspring. The potential underlying mechanism of the ameliorative effect of NaB may be mediated via increasing histone acetylation to the CRHR2 promoter. These results enhance our understanding of the relationship between the SCFAs and the HPA axis in the development of ASD. And gut microbiota-derived SCFAs may serve as a potential therapeutic agent to neurodevelopmental disorders like ASD.
Comparing microscope light-associated glare and comfort between heads-up 3D digital and conventional microscopes in cataract surgery: a randomised, multicentre, single-blind, controlled trial
Authors: Gualino Vincent , Pierne Kevin , Manassero Anthony , Bruneau Sébastien , Couturier Aude , Tadayoni Ramin 
Published: June 2023

ObjectiveTo compare subjective levels of comfort and visual experiences related to microscope light in patients undergoing their first cataract surgery with topical anaesthesia using a digital microscope (the NGENUITY three-dimensional (3D) visualisation system) or a conventional microscope.Methods and analysisA prospective, randomised, single-blinded, parallel-group, multicentre, interventional study. Patients (n=128) were randomly assigned to one of two treatment groups: the experimental group (n=63) had surgery using the digital microscope and the control group (n=65) had surgery with a conventional microscope. The primary outcome was patients’ subjective experience of glare from the microscope light during surgery on a numerical scale from 0 to 10. Key secondary outcomes were patients’ subjective levels of comfort and visual experiences related to the microscope light.ResultsThe experimental group reported significantly lower levels of glare; median levels were 1.0 (0.0–4.0) for the experimental group vs 3.0 (0.0–6.0) for the control group (p=0.027). They also reported higher levels of comfort; median ratings were 8.0 (6.5–10.0) in the experimental group and 7.0 (5.0–9.0) in controls (p=0.026). There were no group differences in ratings of subjective pain or visual disturbances. Median microscope light intensity was lower in the experimental group than controls; 3425.0 (2296.0–4300.0) Lux vs 24 279.0 (16 000.0–26 500.0) Lux (p<0.0001), respectively.ConclusionCompared with conventional microscopes, the NGENUITY 3D visualisation system allows surgeons to operate with lower levels of light exposure, resulting in significantly less glare and improved comfort in patients undergoing cataract surgery.Trial registration numberNCT05085314.
Awareness, attitudes and practices relating to Article 5.3 of the WHO Framework Convention on Tobacco Control among members of tobacco control committees in a southern Indian state
Authors: Kumar Praveen , Kamath Veena Ganesh , Kamath Asha , Kulkarni Muralidhar M , Britton John 
Published: June 2023

Background and objectives
The tobacco industry (TI) has undermined tobacco control policy for decades. The WHO Framework Convention on Tobacco Control Article 5.3 implementation guidelines provide guidance for preventing TI interference. Government officials responsible for policy implementation must understand these guidelines to manage TI tactics. This study assessed awareness, attitudes and practices of Article 5.3 guidelines among members of District Level Coordination Committees (DLCC) in Karnataka mandated with overseeing tobacco control activity.

Method
A semistructured questionnaire survey of awareness, attitudes and adherence to Article 5.3 guidelines among 102 DLCC members carried out between January and July 2019.

Result
Responses were received from 82 members, comprising 51 (62%) from health and 31 (38%) from non-health departments. Our study demonstrates a lack of understanding of Article 5.3 and its guidelines, even among those actively involved in tobacco control at the district level. Nearly 80% of respondents were aware that corporate social responsibility (CSR) by tobacco companies is an indirect form of promoting tobacco. However, 44% of members felt that CSR funding from the TI should be used to combat tobacco-related harm. A higher proportion (12%) of health respondents agreed that subsidies should be provided to tobacco agriculture compared with non-health (3%).

Conclusion
Awareness of international guidance designed to prevent the TI influence on health policy among policymakers in this Indian state is low. Respondents from non-health departments were less aware of TI CSR. Those in health departments were more receptive towards taking a TI role in the future .
Salvaging power-law inflation through warming
Authors: Alhallak M. , Chamoun N. , Eldaher M. S. 
Published: June 2023

AbstractPower-law inflation with scale factor $$a \propto t^m$$

a
∝

t
m

is investigated in the context of warm inflation. The treatment is performed in the weak and strong dissipation limits. In addition, we discuss the three common cases for the thermal dissipation coefficient $$\varGamma (T)$$

Γ
(
T
)

. We compare the theoretical results of the power-law model within warm inflation with the observational constraints from Planck 2018 and BICEP/Keck 2018, as presented by the tensor-to-scalar ratio r and spectral index $$n_s$$

n
s

. The model results agree largely with the observations for most of the $$\varGamma (T)$$

Γ
(
T
)

cases. Furthermore, in order to address the problem of exiting the inflationary epoch, we suggest a perturbed modification to the power-law definition so that it becomes affine, and find that this small change indicates a way for having an exit scenario with a suitable e-foldings number. Finally, we examine this perturbation ansatz within the context of cold inflation with exponential potential, and we find that it can accommodate the observational data with sufficient e-foldings. Our study suggests that the power-law inflation and the exponential potential, in both warm and cold inflation contexts, can in principle be made consistent with the observations and with a possible graceful exit.
Phase-space analysis of a novel cosmological model in f(Q) theory
Authors: Shabani Hamid , De Avik , Loo Tee-How 
Published: June 2023

AbstractThe vanishing affine connections have been used solely while adopting the modified f(Q) gravity theory to the cosmology. Consequently, researchers could not get beyond what is already known in f(T) theory earlier. To alleviate this problem, in the present manuscript we investigate a recently proposed construction of f(Q) theory using non-vanishing affine connection in the spatially flat FLRW spacetime. We then investigate the cosmological solutions of f(Q) theory for a perfect fluid through the phase space analysis. We introduce few variables and dimensionless parameters to construct the corresponding equations suitable for the dynamical system approach. The conservation of the energy-momentum tensor leads to a constraint equation that relates the dynamical variables. Briefly, both unstable and stable de Sitter solutions appear which correspond to early and late times accelerated expansions. Also, unstable points corresponding to the matter dominated and radiation dominated eras have been found which do exist for every f(Q) function. As a result, the present discussion shows that f(Q) gravity endowed by non-vanishing affine connections is capable of explaining a true sequence of cosmic eras.
Axion stars in MGD background
Authors: Casadio R. , da Rocha R. 
Published: June 2023

AbstractThe minimal geometric deformation (MGD) paradigm is here employed to survey axion stars on fluid branes. The finite value of the brane tension provides beyond-general relativity corrections to the density, compactness, radius, and asymptotic limit of the gravitational mass function of axion stars, in a MGD background. The brane tension also enhances the effective range and magnitude of the axion field coupled to gravity. MGD axion stars are compatible to mini-massive compact halo objects for almost all the observational range of brane tension, however, a narrow range allows MGD axion star densities large enough to produce stimulated decays of the axion to photons, with no analogy in the general-relativistic (GR) limit. Besides, the gravitational mass and the density of MGD axion stars are shown to be up to four orders of magnitude larger than the GR axion stars, being also less sensitive to tidal disruption events under collision with neutron stars, for lower values of the fluid brane tension.
Automated verification of countermeasure against detector-control attack in quantum key distribution
Authors: Acheva Polina , Zaitsev Konstantin , Zavodilenko Vladimir , Losev Anton , Huang Anqi , Makarov Vadim 
Published: June 2023

AbstractAttacks that control single-photon detectors in quantum key distribution using tailored bright illumination are capable of eavesdropping the secret key. Here we report an automated testbench that checks the detector’s vulnerabilities against these attacks. We illustrate its performance by testing a free-running detector that includes a rudimentary countermeasure measuring an average photocurrent. While our testbench automatically finds the detector to be controllable in a continuous-blinding regime, the countermeasure registers photocurrent significantly exceeding that in a quantum regime, thus revealing the attack. We then perform manually a pulsed blinding attack, which controls the detector intermittently. This attack is missed by the countermeasure in a wide range of blinding pulse durations and powers, still allowing to eavesdrop the key. We make recommendations for improvement of both the testbench and countermeasure.
Mutant KRAS Mediates circARFGEF2 Biogenesis to Promote Lymphatic Metastasis of Pancreatic Ductal Adenocarcinoma
Authors: Kong Yao , Luo Yuming , Zheng Shangyou , Yang Jiabin , Zhang Dingwen , Zhao Yue , Zheng Hanhao , An Mingjie , Lin Yan , Ai Le , Diao Xiayao , Lin Qing , Chen Changhao , Chen Rufu 
Published: June 2023

Abstract

Circular RNAs (circRNA) contribute to cancer stemness, proliferation, and metastasis. The biogenesis of circRNAs can be impacted by the genetic landscape of tumors. Herein, we identified a novel circRNA, circARFGEF2 (hsa_circ_0060665), which was upregulated in KRASG12D pancreatic ductal adenocarcinoma (PDAC) and positively associated with KRASG12D PDAC lymph node (LN) metastasis. CircARFGEF2 overexpression significantly facilitated KRASG12D PDAC LN metastasis in vitro and in vivo. Mechanistically, circARFGEF2 biogenesis in KRASG12D PDAC was significantly activated by the alternative splicing factor QKI-5, which recruited U2AF35 to facilitate spliceosome assembly. QKI-5 bound the QKI binding motifs and neighboring reverse complement sequence in intron 3 and 6 of ARFGEF2 pre-mRNA to facilitate circARFGEF2 biogenesis. CircARFGEF2 sponged miR-1205 and promoted the activation of JAK2, which phosphorylated STAT3 to trigger KRASG12D PDAC lymphangiogenesis and LN metastasis. Importantly, circARFGEF2 silencing significantly inhibited LN metastasis in the KrasG12D/+Trp53R172H/+Pdx-1-Cre (KPC) mouse PDAC model. These findings provide insight into the mechanism and metastasis-promoting function of mutant KRAS-mediated circRNA biogenesis.

Significance:
Increased splicing-mediated biogenesis of circARFGEF2 in KRAS-mutant pancreatic ductal adenocarcinoma activates JAK2–STAT3 signaling and triggers lymph node metastasis, suggesting circARFGEF2 could be a therapeutic target to inhibit pancreatic cancer progression.
Safety and Efficacy of Tucatinib, Letrozole, and Palbociclib in Patients with Previously Treated HR+/HER2+ Breast Cancer
Authors: Shagisultanova Elena , Gradishar William , Brown-Glaberman Ursa , Chalasani Pavani , Brenner Andrew J. , Stopeck Alison , Parris Hannah , Gao Dexiang , McSpadden Tessa , Mayordomo Jose , Diamond Jennifer R. , Kabos Peter , Borges Virginia F. 
Published: June 2023

Abstract

Purpose:
To overcome resistance to antihormonal and HER2-targeted agents mediated by cyclin D1-CDK4/6 complex, we proposed an oral combination of the HER2 inhibitor tucatinib, aromatase inhibitor letrozole, and CDK4/6 inhibitor palbociclib (TLP combination) for treatment of HR+/HER2+ metastatic breast cancer (MBC).

Patients and Methods:
Phase Ib/II TLP trial (NCT03054363) enrolled patients with HR+/HER2+ MBC treated with ≥2 HER2-targeted agents. The phase Ib primary endpoint was safety of the regimen evaluated by NCI CTCAE version 4.3. The phase II primary endpoint was efficacy by median progression-free survival (mPFS).

Results:
Forty-two women ages 22 to 81 years were enrolled. Patients received a median of two lines of therapy in the metastatic setting, 71.4% had visceral disease, 35.7% had CNS disease. The most common treatment-emergent adverse events (AE) of grade ≥3 were neutropenia (64.3%), leukopenia (23.8%), diarrhea (19.0%), and fatigue (14.3%). Tucatinib increased AUC10–19 hours of palbociclib 1.7-fold, requiring palbociclib dose reduction from 125 to 75 mg daily. In 40 response-evaluable patients, mPFS was 8.4 months, with similar mPFS in non-CNS and CNS cohorts (10.0 months vs. 8.2 months; P = 0.9). Overall response rate was 44.5%, median duration of response was 13.9 months, and clinical benefit rate was 70.4%; 60% of patients were on treatment for ≥6 months, 25% for ≥1 year, and 10% for ≥2 years. In the CNS cohort, 26.6% of patients remained on study for ≥1 year.

Conclusions:
TLP combination was safe and tolerable. AEs were expected and manageable with supportive therapy and dose reductions. TLP showed excellent efficacy for an all-oral chemotherapy-free regimen warranting further testing.
See related commentary by Huppert and Rugo, p. 4993
How Obsessive-Compulsive Symptoms Correlate with other Symptoms and Quality of Life in Schizophrenia? A Cross-Sectional Study
Authors: Awasthi Snigdha , Satapthy Ramananda 
Published: June 2023

Background: Early psychiatrists inferred that obsessive-compulsive symptoms (OCS) represent a defense against psychosis and prevent the progression of schizophrenia. However, recent clinical research has yielded inconsistent results across studies regarding the link between OCS and the symptom domains of schizophrenia and the influence OCS have on a patient’s functioning and quality of life (QOL). This study aimed to determine whether OCS has any association with the symptom severity, functioning, and QOL of schizophrenia patients. Methods: This cross-sectional study done in a tertiary care hospital consecutively recruited 85 outpatients of schizophrenia in clinical remission over one year. Positive and Negative Syndrome Scale (PANSS), Yale-Brown Obsessive Compulsive Scale (Y-BOCS), WHO Quality of Life Brief Version (WHO QOL-BREF), and Global Assessment of Functioning (GAF) scales were applied. Results: Eighteen patients (21.2%) had OCS (Y-BOCS score of ≥8). Patients with OCS had higher PANSS scores (mean±SD of total score 77.16± 16.4, P < 0.001), suggestive of more severe psychosis, poor functioning overall (mean GAF score 37.35 ± 15.62, P < 0.001), and poor QOL in the psychological domain (P < 0.001). In addition, significant associations were present between the Y-BOCS score and the PANSS ( r = 0.65), GAF ( r = –0.61), and WHO QOL BREF scores in the physical ( r = –0.39), and psychological domains ( r = –0.41) (P < 0.001 on all of the above). Conclusions: Patients with OCS have more severe symptoms and a lower QOL. A significant association exists between the intensity of OCS and schizophrenia symptoms, global functioning, and QOL.
Influence of printing-based factors on the mechanical properties of hexagonal lattice-structured 3D printed novel walnut shell/polylactic acid composite
Authors: Mathiazhagan N , Sivakumar Narain Kumar , Palaniyappan Sabarinathan , Rahaman Mostafizur 
Published: June 2023

The incorporation of bio-based natural fillers and lattice structures in thermoplastic polymer has superior advantages such as a reduction in the usage of conventional polymers with improved damping and mechanical properties. The present work focuses on the development of Walnut Shell/PLA (WSP) lattice-structured composite by the Fused Filament Fabrication technique. The flexural strength, compression strength, and thickness error properties of the hexagon lattice-structured WSP composites are optimized by varying Printing-Based Factors (PBFs) which include infill density (60%, 80%, and 100%), nozzle temperature (190°C, 210°C, and 230°C), printing speed (10 mm/sec, 20 mm/sec, and 30 mm/sec), and layer height (0.1 mm, .2 mm, and .3 mm). The flexural and compression test experiments were conducted as per the ASTM D790 and ASTM D695 standards. The process optimization of the measured responses is made by using Taguchi grey relational grade optimization technique. The experimental outcomes such as compressive and flexural strength and minimal dimensional error are converted into the Grey Relational Grade (GRG) for the optimization process. The results show that the Signal-to-Noise ratio (S/N) from the measured GRG clearly shows that, the lower layer height of 0.1 mm, higher nozzle temperature of 230°C, higher infill density of 100%, and average printing speed of 20 mm/sec is an optimal experimental condition for attaining higher strengths and lower errors. From the Analysis of Variance (ANOVA) analysis, the printing temperature, and layer height are the most persuasive PBFs for the mechanical and physical characteristics with respect to the incorporated lattice structure. The optimized experimental conditions develop maximum flexural and compressive strengths of 4.98 MPa and 28.19 MPa, in addition to a lower thickness error of .186 mm obtained for hexagon lattice-structured WSP biopolymeric composites.