bluuebunny/crossref_metadata_2025_split huggingface dataset:
T2T-YAO: a Telomere-to-telomere Assembled Diploid Reference Genome for Han Chinese
Authors: He Yukun , Chu Yanan , Guo Shuming , Hu Jiang , Li Ran , Zheng Yali , Ma Xinqian , Du Zhenglin , Zhao Lili , Yu Wenyi , Xue Jianbo , Bian Wenjie , Yang Feifei , Chen Xi , Zhang Pingan , Wu Rihan , Ma Yifan , Shao Changjun , Chen Jing , Wang Jian , Li Jiwei , Wu Jing , Hu Xiaoyi , Long Qiuyue , Jiang Mingzheng , Ye Hongli , Song Shixu , Li Guangyao , We Yue , Xu Yu , Ma Yanliang , Chen Yanwen , Wang Keqiang , Bao Jing , Xi Wen , Wang Fang , Ni Wentao , Zhang Moqin , Yu Yan , Li Shengnan , Kang Yu , Gao Zhancheng 
Published: July 2023

AbstractSince its initial release in 2001, the human reference genome has been continuously improved in both continuity and accuracy, and the recently-released telomere-to-telomere version—T2T-CHM13—reaches its top quality after 20 years of effort. However, T2T-CHM13 does not represent an authentic diploid human genome, but rather one derived from a simplified, nearly homozygous genome of a hydatidiform mole cell line. To address this limitation and provide an alternative pertinent to the Chinese population, the largest ethnic group in the world, we have assembled a complete diploid human genome of a male Han Chinese, T2T-YAO, which includes telomere-to-telomere assemblies for all the 22+X+M and 22+Y chromosomes in his two haploids inherited separately from his parents. Both haplotypes contain no artificial sequences or model nucleotides and possess a high quality comparable to CHM13, with fewer than one error per ∼14 Mb. Derived from the individual who lives in the aboriginal region of Han Chinese, T2T-YAO shows clear ancestry and potential genetic continuity from the ancient ancestors of the Han population. Each haplotype of T2T-YAO possesses ∼340 Mb exclusive sequences and ∼3100 unique genes as compared to CHM13, and their genome sequences show greater genetic distance to CHM13 than to each other in terms of nucleotide polymorphism and structural variations. The construction of T2T-YAO would serve as a high-quality diploid reference that enables precise delineation of genomic variations in a haplotype-sensitive manner, which could advance our understandings in human evolution, hereditability of diseases and phenotypes, especially within the context of the unique variations of the Chinese population.
Exogenous Methylglyoxal Improves Maize Yield by Alleviating Plant Diabetes and leaf senescence under Drought
Authors: Lin Yi-Hsuan , Zhou Ya-Ning , Jin Yu-ka , Xiao Zu-Dong , Zhang Ying-Jun , Huang Cheng , Hong Bo , Chen Zhen-Yuan , Shen Si , Zhou Shun-Li 
Published: July 2023

AbstractDrought-induced leaf senescence is related to high sugar levels in leaves, photosynthesis inhibition, and ultimate yield loss. This physiological phenomenon in leaves bears resemblance to the symptom of diabetes in human disease. However, the underlying mechanisms of plant diabetes on carbon imbalance in maize leaf and corresponding detoxification strategy have not been well understood. In this study, we demonstrated that foliar application of exogenous methylglyoxal (MG) delayed leaf senescence and promoted photoassimilation, retrieved 14% yield loss induced by drought stress during grain filling stage. Transcriptome and metabolite analysis revealed that drought increased sugar accumulation in leaf with inhibition of sugar transporters facilitating phloem loading. This further lead to disequilibrium of glycolysis and over-accumulation of endogenous MG. Contrarily, exogenous MG significantly upregulated glycolytic flux and glyoxalase system catabolizing endogenous MG and advanced glycation end products toxicity, ultimately alleviating plant diabetes. Besides, the genes facilitating anabolism and catabolism of trehalose- 6-phosphate were promoted and suppressed by drought, respectively, whereas exogenous MG reversed the effect. Moreover, exogenous MG activated phenylpropanoid biosynthetic pathway, likely promoting cell structural integrity. Collectively, these results suggest that exogenous MG alleviates the toxic effect from drought-induced sugar accumulation and activates the defense-related pathway, thereby maintaining leaf function and yield production.HighlightExogenous methylglyoxal stimulates glycolytic flux and glyoxalase system, providing a potential insight to alleviate plant diabetes under drought condition.
A prospective case series assessing the Imperial College London intraoperative radiotherapy protocol for advanced rectal cancer: an IDEAL phase 2a study
Authors: Murphy Jamie , Donato‐Brown Daniella , Efeotor Ouvefe , Gilfillan Nicholas , Moutadjer Anne , Sahid Saidah , Basak Dolan , Perry Kitrick 
Published: July 2023

AbstractAimIntraoperative radiotherapy (IORT) decreases local recurrence rates for advanced rectal cancer. Nevertheless, utilization of IORT is limited due to the associated logistical and financial challenges. The aim of this study is to describe the development and delivery of a novel IORT protocol for advanced rectal cancer that overcomes these difficulties in the context of the early phase of an IDEAL 2a study. The primary outcome measure was the ability to deliver IORT using this novel protocol with IORT‐related toxicity as the secondary outcome measure.MethodConsecutive patients with advanced rectal cancer expected to have involved (R1) resection margins were enrolled. After resection, 12 Gy low‐energy photon IORT was delivered using the Axxent device with a custom‐designed elliptical spherical balloon applicator.ResultsSix patients with a median age of 47 years (range 33–88 years; five women) were enrolled between 2018 and 2019. The indication was advanced cancer in four patients and atypical invasive pelvic side wall lymph nodes in two. IORT was successfully delivered in all cases. No toxicity was encountered. Three advanced cancer patients had R1 resection and one had complete resection (R0); resection margin status could not be established for the two lymph node cases. Five patients were alive at 3.4 (2.9–4.1) year follow‐up. None of the R1 cases recurred.ConclusionThis is the first study to describe a novel IORT protocol using low‐energy photon IORT for advanced rectal cancer. IORT could be delivered in all cases and no IORT‐related toxicity was encountered. Available oncological outcome data are encouraging, but further studies will be necessary to determine the oncological effectiveness of this protocol.
An Approach for Fast-Charging Lithium-Ion Batteries State of Health Prediction Based on Model-Data Fusion
Authors: Feng Hailin , Liu Yatian 
Published: July 2023

Abstract
Fast charging has become the norm for various electronic products. The research on the state of health prediction of fast-charging lithium-ion batteries deserves more attention. In this paper, a model-data fusion state of health prediction method which can reflect the degradation mechanism of fast-charging battery is proposed. First, based on the Arrhenius model, the log-power function (LP) model and log-linear (LL) model related to the fast-charging rate are established. Second, combined with Gaussian process regression prediction, a particle filter is used to update the parameters of models in real-time. Compared with the single Gaussian process regression, the average root-mean-square error of LP and LL is reduced by 71.56% and 69.11%, respectively. Finally, the sensitivity and superiority of the two models are analyzed by using Sobol method, Akaike and Bayesian information criterion. The results show that the two models are more suitable for fast-charging lithium batteries than the traditional Arrhenius model, and LP model is better than LL model.
Non-targeted multimodal metabolomics data from ovine rumen fluid fractions
Authors: Palevich Nikola , Maclean Paul H. , Cao Mingshu 
Published: July 2023

ABSTRACT
From an animal health perspective, our understanding of the metabolites in rumen fluid across different host species is poorly understood. Here, we present a metabolomic data set generated using hydrophilic interaction liquid chromatography and semi-polar (C18) chromatography methods coupled to high-resolution mass spectrometry of fractionated ovine rumen samples.
Estimating the economic burden of long-Covid: the additive cost of healthcare utilisation among COVID-19 recoverees in Israel
Authors: Wolff Sagy Yael , Feldhamer Ilan , Brammli-Greenberg Shuli , Lavie Gil 
Published: July 2023

IntroductionPostacute sequelae resulting from SARS-CoV-2 infections (LONG-COVID) have been reported. The resulting added economic burden from the perspective of healthcare organisations is not clear. Therefore, this study aims to evaluate the additive healthcare costs among COVID-19 recoverees, in a large community-dwelling general population, as incurred by an insurer-provider organisation over time.MethodsIn this historical cohort study, cost data from Clalit Health Services (CHS) were analysed. The primary endpoint was the direct cost incurred by CHS per month per person. Costs were measured for COVID-19 recoverees and matched controls, from January 2019 to January 2022. Difference in differences (DiDs) were calculated as the difference in mean monthly costs in cases and controls in the post-COVID-19 individual period, deducing their cost difference in a prepandemic 12 months baseline period.ResultsAmong N=642 868 community-dwelling COVID-19 recoverees, 268 948 (40.8%) were 0–19 years old and 63 051 (9.6%) were 60 years or older. A total of 16 017 (2.5%) of recoverees had been hospitalised during the acute phase of the COVID-19 disease. Costs in cases and controls converged after 16 months from recovery. The mean monthly cost incurred by CHS per COVID-19 recoverees over up to 15 months (mean: 8.25) of post-COVID-19 follow-up was higher by 8.2% (US$8.2) compared with matched controls. The excess cost attributable to post-COVID-19 effects (DID) was 7.6% of the cost in controls (US$7.7 per patient per month). Both net and relative DIDs were substantially higher in patients who required hospitalisation during the acute phase of COVID-19 and in older adults. Excess in hospitalisations, primary care physicians and medical specialists’ visits-related costs were observed.ConclusionsLong-term effects of SARS-CoV-2 infections translate into excess healthcare costs, months after recovery, hence requiring adjustments of funds allocation. These excess costs gradually diminish after recoveree, returning to baseline differences 16 months after recoveree.
Symptoms of eating disorders and low energy availability in recreational active female runners
Authors: Karlsson Elin , Alricsson Marie , Melin Anna 
Published: July 2023

ObjectivesThis retrospective, cross-sectional study aimed to investigate symptoms of eating disorders (EDs) and low energy availability (LEA) among recreational female runners.MethodsFemales (18–39 years) (n=89) participating in running group sessions organised by running clubs and companies were recruited via social media and completed an anonymous online survey compromising the Eating Disorder Examination Questionnaire (EDE-Q) and Low Energy Availability in Females Questionnaire (LEAF-Q). An EDE-Q global score ≥2.3 and a LEAF-Q total score ≥8 (in combination with an injury score≥2 and/or menstruation dysfunction score≥4) were used to categorise subjects as having symptoms of EDs and LEA, respectively.ResultsAmong the subjects fulfilling the age criteria (n=85), 18% (n=15) had symptoms of EDs and 19% (n=16) had symptoms of LEA. Of those with symptoms of EDs, 13% (n=2) had concomitant symptoms of LEA. The higher the EDE-Q dietary restraint score, the higher the gastrointestinal problem score (r=0.23, p=0.04), otherwise no other associations were found between EDE-Q global or subscale scores and LEAF-Q scores.ConclusionOur results indicate that symptoms of EDs and LEA are frequent among adult females at all athletic levels, including the recreational level. Hence, to prevent the negative health consequences of EDs and LEA, preventative initiatives are also needed in recreational running communities.
The interaction between random and systematic visual stimulation and
infraslow quasi-periodic spatiotemporal patterns of whole-brain
activity
Authors: Xu Nan , Smith Derek M. , Jeno George , Seeburger Dolly T. , Schumacher Eric H. , Keilholz Shella D. 
Published: July 2023

Abstract
One prominent feature of the infraslow blood oxygen level-dependent (BOLD) signal during rest or task is quasi-periodic spatiotemporal pattern (QPP) of signal changes that involves an alternation of activity in key functional networks and propagation of activity across brain areas, and that is known to tie to the infraslow neural activity involved in attention and arousal fluctuations. This ongoing whole-brain pattern of activity might potentially modify the response to incoming stimuli or be modified itself by the induced neural activity. To investigate this, we presented checkerboard sequences flashing at 6 Hz to subjects. This is a salient visual stimulus that is known to produce a strong response in visual processing regions. Two different visual stimulation sequences were employed, a systematic stimulation sequence in which the visual stimulus appeared every 20.3 s and a random stimulation sequence in which the visual stimulus occurred randomly every 14~62.3 s. Three central observations emerged. First, the two different stimulation conditions affect the QPP waveform in different aspects; i.e., systematic stimulation has greater effects on its phase and random stimulation has greater effects on its magnitude. Second, the QPP was more frequent in the systematic condition with significantly shorter intervals between consecutive QPPs compared to the random condition. Third, the BOLD signal response to the visual stimulus across both conditions was swamped by the QPP at the stimulus onset. These results provide novel insights into the relationship between intrinsic patterns and stimulated brain activity.
Shifting the Paradigm: Cultivating Socially Responsible Atmospheric Scientists through Leadership and Action
Authors: Burt Melissa A. , Fischer Emily V. , Rasmussen Kristen L. , Crosley Beem Katie 
Published: July 2023

Abstract
The geosciences have the lowest racial and ethnic diversity of all STEM fields at all levels of higher education, and atmospheric science is emblematic of this discrepancy. Despite a growing awareness of the problem, Black, Indigenous, people of color, persons with disabilities, women, and LGBTQIA+ persons continue to be largely absent in academic programs and in the geoscience workforce. There is a desire and need for new approaches, new entry points, and higher levels of engagement to foster a diverse community of researchers, scholars, and practitioners in atmospheric science. One challenge among many is that diversity, equity, and inclusion efforts are often siloed from many aspects of the scientific process, technical training, and scientific community. We have worked toward bridging this gap through the development of a new atmospheric science course designed to break down traditional barriers for entry into diversity, equity, and inclusion engagement by graduate students, so they emerge better prepared to address issues of participation, representation, and inclusion. This article provides an overview of our new course, focused on social responsibility in atmospheric science. This course was piloted during Fall 2021 with the primary objective to educate and empower graduate students to be “diversity champions” in our field. We describe 1) the rationale for a course of this nature within a graduate program, 2) course design and content, 3) service-learning projects, 4) impact of the course on students, and 5) scalability to other atmospheric science graduate programs.
Pioneer Arabidopsis thaliana spans the succession gradient revealing a diverse root-associated microbiome
Authors: Hesen Vera , Boele Yvet , Bakx-Schotman Tanja , van Beersum Femke , Raaijmakers Ciska , Scheres Ben , Willemsen Viola , van der Putten Wim H. 
Published: July 2023

Abstract
Background
Soil microbiomes are increasingly acknowledged to affect plant functioning. Research in molecular model species Arabidopsis thaliana has given detailed insights of such plant-microbiome interactions. However, the circumstances under which natural A. thaliana plants have been studied so far might represent only a subset of A. thaliana’s full ecological context and potential biotic diversity of its root-associated microbiome.

Results
We collected A. thaliana root-associated soils from a secondary succession gradient covering 40 years of land abandonment. All field sites were situated on the same parent soil material and in the same climatic region. By sequencing the bacterial and fungal communities and soil abiotic analysis we discovered differences in both the biotic and abiotic composition of the root-associated soil of A. thaliana and these differences are in accordance with the successional class of the field sites. As the studied sites all have been under (former) agricultural use, and a climatic cline is absent, we were able to reveal a more complete variety of ecological contexts A. thaliana can appear and sustain in.

Conclusions
Our findings lead to the conclusion that although A. thaliana is considered a pioneer plant species and previously almost exclusively studied in early succession and disturbed sites, plants can successfully establish in soils which have experienced years of ecological development. Thereby, A. thaliana can be exposed to a much wider variation in soil ecological context than is currently presumed. This knowledge opens up new opportunities to enhance our understanding of causal plant-microbiome interactions as A. thaliana cannot only grow in contrasting soil biotic and abiotic conditions along a latitudinal gradient, but also when those conditions vary along a secondary succession gradient. Future research could give insights in important plant factors to grow in more ecologically complex later-secondary succession soils, which is an impending direction of our current agricultural systems.
Box–Behnken design-assisted optimization of RP-HPLC method for the estimation of evogliptin tartrate by analytical quality by design
Authors: Patel Khushbu , Shah Ujashkumar A. , Patel C. N. 
Published: July 2023

Abstract
Background
A quality by design approach can potentially lead to a more robust/rugged method development due to emphasis on the risk assessment and management. By carefully understanding the step-by-step procedure for analytical QbD-based optimization parameters, such as analytical target profile and critical quality attributes (CQAs), was assessed. The present study describes the simple, rapid, sensitive and cost-effective RP-HPLC method development and validation for the estimation of evogliptin tartrate in pharmaceutical dosage form.

Results
The factor screening studies were performed using Box–Behnken design by three key components of the RP-HPLC method (mobile phase, pH and flow rate). The chromatographic conditions were optimized with the Design Expert software trial version 13.0. The optimal chromatographic separation was achieved having water C18 column (250 mm × 4.6 mm, 5 μ) and using mobile phase as a methanol and phosphate buffer (pH 4.5) 60:40% v/v with a flow rate 1.0 ml/min and UV detection at 267 nm. The Box–Behnken experimental design describes the interrelationship of mobile phase, pH and flow rate at three different levels, and responses of retention time and tailing factor were observed with response surface plot and statistical data. The developed method was validated as per recommended ICH guidelines which revealed the high degree of linear, precise, accurate, sensitive and robust method over the existing RP-HPLC method for evogliptin tartrate.

Conclusion
The developed QbD-based method helped in generating a design space and operating space with knowledge of all method performance characteristics, and RP-HPLC method takes less time and can be used in the industry for routine quality control of bulk and marketed formulation of evogliptin tartrate.
Eye movement desensitisation and reprocessing: part 3 – applications in physical health conditions
Authors: Udo Itoro , Javinsky Tori-Rose , McDaniel Carol 
Published: July 2023

SUMMARYEye movement desensitisation and reprocessing (EMDR) is a psychological therapy that addresses trauma, stress and emotional distress. It has been successfully used in the management of various psychiatric disorders. This article shows that it may also be safely used to manage the psychological distress arising from a variety of physical health conditions and in so doing, reduce the illness burden from conditions such as various cancers, traumatic childbirth, tokophobia, pre-eclampsia, myocardial infarction, haemodialysis in end-stage renal disease, and acute postoperative pain. It can be a stand-alone treatment for hyperemesis gravidarum and tinnitus. The article examines the rationale and evidence for its use in these conditions and suggests areas where more research is needed. Adding EMDR therapy to the range of available interventions in general hospitals has the potential to improve the health and well-being of patients in these settings.
Study of women's knowledge of the role of androgens and their excess in women - research article
Authors: Ćwik-Błotnicka Dominika , Nitka Karolina 
Published: July 2023

Introduction: excess androgens is one of the most common endocrine disorder in women. The ovaries and/or adrenal glands are responsible for the excess of androgens. The most common cause is polycystic ovary syndrome.
The aim of the study: was to show the level of knowledge of the women surveyed about the role of androgens and to find out the degree of their awareness of androgen excess in women. Last but not least, it was important to find out which symptoms the respondents think are characteristic of androgen excess in women, whether they know the concept of hirsutism and what androgenetic alopecia looks like.
Material and method: wykorzystano ankietę własnego autorstwa. Przebadano 172 kobiety. Dokonano porównania odpowiedzi na pytania pomiędzy podgrupami. Oparto się na aktualnej literaturze medycznej oraz artykułach oryginalnych i badawczych znalezionych w PubMed.
Results: slightly more than half of those surveyed know that testosterone is produced by the ovaries and adrenal cortex. Only 35.5% of the women surveyed can name other hormones belonging to the androgens besides testosterone. 60.7% of women know the characteristic symptoms of hyperandrogenism. 51.7% know the term hirsutism and 32% correctly identify the most common cause.
Conclusions: given the prevalence of endocrine disorders in women, it is important to expand and disseminate information on the symptoms and causes of androgen excess in women, especially among women without medical education.
Molecular studies of coralberry (Ardisia crenata Sims; Primulaceae) from Thailand based on SCoT markers
Authors: SUPARMAN SUPARMAN , PORNPONGRUNGRUENG PIMWADEE , CHANTARANOTHAI PRANOM 
Published: July 2023

Abstract. Suparman S, Pornpongrungrueng P, Chantaranothai P. 2023. Molecular studies of coralberry (Ardisia crenata Sims; Primulaceae) from Thailand based on SCoT markers. Biodiversitas 24: 3183-3189. A coralberry (Ardisia crenataSims) is the most common species of Ardisia Sw. distributed widely in Thailand. The grouping of the species for intraspecific based on morphometric characters was unstable. Some molecular primers were used by researchers to separate species. In this molecular study, six selected SCoT of 36 primers, i.e., primers no. 13, 14, 15, 19, 20, and 21, displayed high polymorphic and resolving power in A. crenata from 14 different natural populations in Thailand. This marker separated all taxa into two groups from different floristic regions. In contrast, this result did not support the classification of the intraspecific level of A. crenata based on continued morphological characteristics. For the conclusion that this study was recorded as the first one using the SCoT marker on the Ardisiaand the primer worked well in A. crenata and was recommended for other species in Ardisia.