bluuebunny/crossref_metadata_2025_split huggingface dataset:
Mid-term and Long-term Outcomes After Total Cervical Disk Arthroplasty Compared With Anterior Cervical Discectomy and Fusion
Authors: Kim Leo J.Y. , Mazur Marcus D. , Dailey Andrew T. 
Published: September 2023

Study Design:
A meta-analysis of randomized controlled trials (RCTs).

Objective:
The aim of this study was to compare mid-term to long-term outcomes of cervical disk arthroplasty (CDA) with those of anterior cervical discectomy and fusion (ACDF) for the treatment of symptomatic cervical degenerative disk disease.

Summary of Background Data:
After ACDF to treat symptomatic cervical degenerative disk disease, the loss of motion at the index level due to fusion may accelerate adjacent-level disk degeneration. CDA was developed to preserve motion and reduce the risk of adjacent segment degeneration. Early-term to mid-term clinical outcomes from RCTs suggest noninferiority of CDA compared with ACDF, but it remains unclear whether CDA yields better mid-term to long-term outcomes than ACDF.

Materials and Methods:
Two independent reviewers searched PubMed, Embase, and the Cochrane Library for RCTs with at least 60 months of follow-up. The risk ratio or standardized mean difference (and 95% CIs) were calculated for dichotomous or continuous variables, respectively.

Results:
Eighteen reports of 14 RCTs published in 2014–2023 were included. The pooled analysis demonstrated that the CDA group had a significantly greater improvement in neurological success and Neck Disability Index than the ACDF group. The ACDF group exhibited a significantly better improvement in the Short Form-36 Health Survey Physical Component Summary than the CDA group. Radiographic adjacent segment degeneration was significantly lower in the CDA group at 60- and 84-month follow-ups; at 120-month follow-up, there was no significant difference between the 2 groups. Although the overall rate of secondary surgical procedures was significantly lower in the CDA group, we did not observe any significant difference at 60-month follow-up between the CDA and ACDF group and appreciated statistically significant lower rates of radiographic adjacent segment degeneration, and symptomatic adjacent-level disease requiring surgery at 84-month and 108- to 120-month follow-up. The rate of adverse events and the neck and arm pain scores in the CDA group were not significantly different from those of the ACDF group.

Conclusions:
In this meta-analysis of 14 RCTs with 5- to 10-year follow-up data, CDA resulted in significantly better neurological success and Neck Disability Index scores and lower rates of radiographic adjacent segment degeneration, secondary surgical procedures, and symptomatic adjacent-level disease requiring surgery than ACDF. ACDF resulted in improved Short Form-36 Health Survey Physical Component Summary scores. However, the CDA and ACDF groups did not exhibit significant differences in overall changes in neck and arm pain scores or rates of adverse events.
One-Year Data on the Longevity and Safety of Hyaluronic Acid Filler for Static Horizontal Neck Rhytids
Authors: Siperstein Robyn , Nestor Elizabeth , Meran Suzanne , Grunebaum Lisa , Cotofana Sebastian 
Published: September 2023

BACKGROUNDThe long-term efficacy and safety of hyaluronic acid fillers injected into the neck have yet to be analyzed in a prospective trial in the United States.OBJECTIVEAnalyze the long-term efficacy and safety of a hyaluronic acid filler for static horizontal neck rhytids.MATERIALS AND METHODSTwenty-six subjects were enrolled in the study. Six subjects were randomized to receive saline and 20 subjects were randomized to receive HARR.All subjects were randomized to use a cannula on 1 side and needle on the other with optional retreatment on day 30 and optional crossover treatment with different assignment on day 60. The subjects were graded using the Transverse Neck Line Scale, Canfield photography, and the Global Aesthetic Improvement Scale on day 360.RESULTSA significant improvement from baseline on day 360 was achieved on the Transverse Neck Line Scale according to the blind evaluator, subjects, and investigator without any delayed-onset or prolonged side effects. There was no significant difference in the average improvement from the short-term analysis on day 60 to the long-term analysis on day 360.CONCLUSIONHARRachieved significant long-term improvement in static horizontal neck rhytids without any long-term side effects.
An Update and Review of Clinical Outcomes Using Immunohistochemical Stains in Mohs Micrographic Surgery for Melanoma
Authors: Elgash May , Young Jade , White Kevin , Leitenberger Justin , Bar Anna 
Published: September 2023

BACKGROUNDMohs micrographic surgery (MMS) provides optimal margin control through complete peripheral and deep margin assessment. The treatment of melanoma using MMS has historically been limited by difficulty in interpreting melanocytes using frozen sections. Immunohistochemical (IHC) staining, a technique whereby chromogen-tagged antibodies are used to detect antigens of interest, has revolutionized the surgical treatment of melanoma.OBJECTIVESThis article provides an update and literature review of current IHC stains used in MMS for melanoma, their sensitivities and specificities, and clinical outcomes.MATERIALS AND METHODSA PubMed search was performed using keywords including “immunohistochemistry,” “staining,” and “Mohs surgery.” Articles related to the use of IHC staining for the treatment of melanoma with MMS were included.RESULTSSix IHC stains met the criteria for the review including melanoma antigen recognized by T cells (MART-1), SRY-related HMG-box (SOX10), microphthalmia-associated transcription factor, HMB-45, MEL-5, S-100, and preferentially expressed antigen in melanoma.CONCLUSIONThe adaptation of IHC methods to frozen sections has enabled MMS to become a preferred treatment option for melanoma in special-site areas. Future studies are needed to standardize IHC techniques and to define best practices when using frozen section in the treatment of melanoma.
Implementation of an Interprofessional Mobility Program in a Neurosurgical Intensive Care Unit
Authors: Broadway Katie , Nuila Cristina M. 
Published: September 2023

ABSTRACT

BACKGROUND: Emerging research suggests the need for implementation of mobility protocols and consistent evaluation of the impact on patient outcomes. Standardized mobility guidelines may be a solution for promoting nurse-driven mobility efforts and influencing a shift in the culture of mobility among nursing teams. In a 36-bed neurosurgical intensive care unit, 2 key areas of opportunity were identified related to patient mobility: strengthening of frontline nursing engagement in mobility and accuracy of documented early and safe mobilization. METHODS: Using a plan-do-study-act performance improvement framework, an interprofessional team developed a comprehensive early patient mobility program. Defined criteria and mobility rounds assisted in identifying the patient's functional ability and level of assistance. Nursing staff received education in case study format to enhance understanding and improve practical application of the phases of mobility. Chart reviews served as continuous assessment of documented mobilizations within the appropriate phases of mobility, and surveys evaluated staff perceptions of program success. RESULTS: On a rating scale of 1 to 5, clinicians reported a rating of 4.32 in overall observation that patients are safely mobilized earlier and more frequently and 4.48 on overall improvement in the neurosurgical intensive care unit culture of mobility. CONCLUSION: An interprofessional mobility program focusing on safe and early mobilization may improve overall culture, confidence, and empowerment of the frontline clinicians.
Preoperative carbohydrate antigen 19.9 level predicts lymph node metastasis in resectable adenocarcinoma of the head of the pancreas: a further plea for biological resectability criteria
Authors: Coppola Alessandro , La Vaccara Vincenzo , Farolfi Tommaso , Asbun Horacio J. , Boggi Ugo , Conlon Kevin , Edwin Bjørn , Ferrone Cristina , Jonas Eduard , Kokudo Norihiro , Perez Elena Martin , Satoi Sohei , Sparrelid Ernesto , Stauffer John , Zerbi Alessandro , Takemura Nobuyuki , Lai Quirino , Almerey Tariq , Bernon Marc , Cammarata Roberto , Djoumi Yasmine , Gallagher Tom , Ghorbani Poya , Ginesini Michael , Hashimoto Daisuke , Kauffmann Emanuele F , Kleive Dyre , Lluís Núria , González Rocio Maqueda , Napoli Niccolò , Nappo Gennaro , Nebbia Martina , Ricchitelli Simone , Sahakyan Mushegh A. , Yamamoto Tomohisa , Coppola Roberto , Caputo Damiano 
Published: September 2023

Introduction:
Lymph-nodal involvement (N+) represents an adverse prognostic factor after pancreatoduodenectomy (PD) for pancreatic adenocarcinoma (PDAC). Preoperative diagnostic and staging modalities lack sensitivity for identifying N+. This study aimed to investigate preoperative carbohydrate antigen 19.9 (CA 19.9) in predicting the N+ stage in resectable-PDAC (R-PDAC).

Methods:
Patients included in a multi-institutional retrospective database of PDs performed for R-PDAC from January 2000 to June 2021 were analysed. A preoperative laboratory value of CA 19.9 greater than 37 U/l was used in univariate and multivariate logistic regression analysis to determine a possible association with N+. Additionally, different cut-offs of CA 19.9 related to the preoperative clinical T (cT) stage was assessed to evaluate the risk of N+.

Results:
A total of 2034 PDs from thirteen centres were included in the study. CA 19.9 greater than 37 U/l was significantly associated with higher N+ at univariate and multivariate analysis (P<0.001). CA 19.9 levels greater than 37 U/l were associated with N+ in 75.9%, 81.3%, and 85.7% of patients, respectively, in cT1, cT2, and cT3 tumours and with higher cut-off values for all cT stages.

Conclusion:
Lymph-nodal involvement is strongly related to preoperative CA 19.9 levels. Specially in patients staged as cT3 the CA 19.9 could represent a valid and easy tool to suspect nodal involvement. Due to these findings, R-PDAC patients with elevated CA 19.9 values should be considered in a more biologically advanced stage.
Pediatric Balance Scale: Translation and Cross-Cultural Adaptation Into Greek
Authors: Bania Theofani A. , Gkoutsidou Paraskevi , Billis Evdokia , Lampropoulou Sofia 
Published: September 2023

Purpose:
Cross-cultural adaptation of the Pediatric Balance Scale (PBS) into Greek.

Methods:
The PBS was forward-back translated and evaluated for content equivalence. The Greek PBS (PBSGR) was administered to children with movement impairments by 2 pediatric physical therapists. The scale was readministered to the same children after 3 weeks (test-retest reliability) and to children with typical development for discriminant validity. The 1-minute walk test was administered to test the scale's concurrent validity.

Results:
Psychometric testing was completed on 26 children with movement impairments. The scale had excellent interrater and test-retest reliability and internal consistency. Moderate correlation was observed between PBSGR and 1-minute walk. Children with movement impairment had significantly lower PBSGR scores than children with typical development.

Conclusions:
Acceptable reliability, concurrent validity, and discriminant validity were observed for the PBSGR.
Fidelity of Delivery in a Multisite Randomized Clinical Trial of Intervention Efficacy for Infants With Unilateral Cerebral Palsy
Authors: Scott Kimberley , Sakzewski Leanne , Ziviani Jenny , Heathcock Jill C. , Boyd Roslyn N. 
Published: September 2023

Purpose:
To investigate the reliability of a measure of fidelity of therapist delivery, quantify fidelity of delivery, and determine factors impacting fidelity in the Rehabilitation EArly for Congenital Hemiplegia (REACH) clinical trial.

Methods:
Ninety-five infants (aged 3-9 months) with unilateral cerebral palsy participated in the REACH clinical trial. The Therapist Fidelity Checklist (TFC) evaluated key intervention components. Video-recorded intervention sessions were scored using the TFC.

Results:
Inter- and intrarater reliability was percentage agreement 77% to 100%. Fidelity of delivery was high for 88.9% of sessions and moderate for 11.1% of sessions. Sessions with moderate scores included infants receiving infant-friendly bimanual therapy and occurred at the intervention midpoint or later. No significant relationships were found for TFC scores and infant age, manual ability, or parent engagement.

Conclusions:
Fidelity of delivery was high for the REACH trial in most intervention sessions. Standardized therapist training with intervention manuals and monthly peer-to-peer support likely contributed to these results.
Daily Quantity and Kinematic Characteristics of Leg Movement in a Child With SMA (2 Copies SMN2)
Authors: McIntyre Melissa , Dunn Lauren , David Jed , Devine Ceri , Smith Beth A. 
Published: September 2023

Purpose:
This case report describes daily leg movement quantity and kinematic characteristics of a child with spinal muscular atrophy (SMA) with 2 copies of SMN2, who was symptomatic at the time of treatment with disease-modifying therapies.

Key Points:
Compared with infants with typical development, this child had differing values for leg movement quantity, duration, average acceleration, and peak acceleration measured across full days in the natural environment by wearable sensors. In addition, movement quantity and clinician-rated outcomes increased with age.

Conclusions:
Wearable sensors recorded movement quantity and kinematic characteristics in a treated infant with SMA (2 copies SMN2). These movement parameters were consistently different compared anecdotally with published data from infants with typical development, demonstrating their potential to add unique and complementary information to the assessment of motor function in SMA.

Recommendations:
Larger longitudinal studies are needed to determine the utility of wearable sensors as an assessment tool and an early predictor of motor outcomes in children with SMA.
Additive Orthodiagonal Rhytidoplasty of the Ergotrid: A Novel Treatment Technique for Severe Dynamic Lip Lines
Authors: Soares Danny J. , Bowhay Alexis 
Published: September 2023

Summary:
Dynamic lip lines pose a challenge in perioral rejuvenation despite the availability of numerous hyaluronic acid dermal filler products and techniques. In this article, the authors introduce additive orthodiagonal rhytidoplasty of the ergotrid, or ADORE, a novel technique for the treatment of severe dynamic lip lines. Based on an objective analysis of treatment outcomes, the authors’ technique demonstrates the ability to confer substantial improvement in dynamic lip line severity, with a median reduction in lip line intensity of 79% (average, 70%; SD, 19%) at 1 month and 72% (average, 61%; SD, 21%) at 6 months, and a high degree of patient satisfaction.
Oryzibacter oryziterrae gen. nov., sp. nov., isolated from rice paddy soil
Authors: Lee Hyo-Jin , Whang Kyung-Sook 
Published: September 2023

A novel Gram-stain-negative, aerobic, motile and pleomorphic rod-shaped bacterial strain, designated COJ-58T, was isolated from rice paddy soil. Strain COJ-58T grew optimally at 20–30 °C, at pH 5.0–8.0 and with 0–1.0 % (w/v) NaCl. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain COJ-58T forms a distinct lineage within the family

Pleomorphomonadaceae

, with highest similarity to

Pleomorphomonas carboxyditropha

SVCO-16T (95.9 %),

Pleomorphomonas koreensis

Y9T (95.8 %),

Pleomorphomonas oryza

e F-7T (95.7 %) and

Pleomorphomonas diazotrophica

R5-392T (95.6 %), respectively. The average nucleotide identity, digital DNA–DNA hybridization, average amino acid identity and percentage of conserved proteins values between the genomes of strain COJ-58T and its closely related taxa are ≤77.2 %, ≤21.6 %, ≤68.3 % and ≤61.3 %, respectively. The genome size of strain COJ-58T is 4.9 Mb and the genomic DNA G + C content is 63.7 mol%. The major fatty acids are C18 : 1
ω7c, C16 : 0 and summed feature 2 (C14 : 0 3-OH and/or iso-C16 : 1 I). The differential phenotypic and genotypic characteristics of strain COJ-58T indicate that it represents a novel genus and species, for which the name Oryzibacter oryziterrae gen. nov., sp. nov. is proposed, with strain COJ-58T (=KACC 22108T=JCM 34744T) as the type strain.
High standing diversity masks extreme genetic erosion in a declining snake
Authors: Pozzi Andrea Vittorio , Owens John Benjamin , Üveges Bálint , Major Tom , Morris Ellie , Graham Stuart , Togridou Anatoli , Papadopulos Alexander S.T. , Wüster Wolfgang , Barlow Axel 
Published: September 2023

ABSTRACTAverage heterozygosity is frequently used as a proxy for genetic health, and to compare genetic diversity between species and populations. However, this measurement could be misleading if the distribution of heterozygosity across the genome is highly skewed. We investigated this pitfall in methodology using whole-genome sequencing of the adder (Vipera berus), a species experiencing dramatic declines in the UK. We find that mean heterozygosity in adders is notably high, exceeding that of other vertebrates typically regarded as genetically diverse. Their genome-wide distribution of heterozygosity, however, approximates a negative exponential distribution, with most genome regions showing extremely low heterozygosity. Modelling approaches show that this pattern is likely to have resulted from a recent, severe bottleneck and fragmentation most likely caused by anthropogenic activity in a previously large, interconnected adder population. Our results highlight that high standing diversity may mask severe genetic erosion when declines are recent and rapid. In such situations, whole-genome sequencing may provide the best option for genetic risk assessment and targeted conservation actions.
Capture of circulating metastatic cancer cell clusters from a lung cancer patient can reveal a unique genomic profile and potential anti-metastatic molecular targets: A proof of concept study
Authors: Kouhmareh Kourosh , Martin Erika , Finlay Darren , Bhadada Anukriti , Hernandez-Vargas Hector , Downey Francisco , Allen Jeffrey K. , Teriete Peter 
Published: September 2023

AbstractMetastasis remains the leading cause of cancer deaths worldwide and lung cancer, known for its highly metastatic progression, remains among the most lethal of malignancies. The heterogeneous genomic profile of lung cancer metastases is often unknown. Since different metastatic events can selectively spread to multiple organs, strongly suggests more studies are needed to understand and target these different pathways. Unfortunately, access to the primary driver of metastases, the metastatic cancer cell clusters (MCCCs), remains difficult and limited. These metastatic clusters have been shown to be 100-fold more tumorigenic than individual cancer cells. Capturing and characterizing MCCCs is a key limiting factor in efforts to help treat and ultimately prevent cancer metastasis. Elucidating differentially regulated biological pathways in MCCCs will help uncover new therapeutic drug targets to help combat cancer metastases. We demonstrate a novel, proof of principle technology, to capture MCCCs directly from patients’ whole blood. Our platform can be readily tuned for different solid tumor types by combining a biomimicry-based margination effect coupled with immunoaffinity to isolate MCCCs. Adopting a selective capture approach based on overexpressed CD44 in MCCCs provides a methodology that preferentially isolates them from whole blood. Furthermore, we demonstrate a high capture efficiency of more than 90% when spiking MCCC-like model cell clusters into whole blood. Characterization of the captured MCCCs from lung cancer patients by immunofluorescence staining and genomic analyses, suggests highly differential morphologies and genomic profiles., This study lays the foundation to identify potential drug targets thus unlocking a new area of anti-metastatic therapeutics.
Voxelated bioprinting of modular double-network bio-ink droplets
Authors: Zhu Jinchang , He Yi , Wang Yong , Cai Li-Heng 
Published: September 2023

AbstractAnalogous of pixels to two-dimensional pictures, voxels –– in the form of either small cubes or spheres –– are the basic building blocks of three-dimensional (3D) objects. However, precise manipulation of viscoelastic bio-ink voxels in 3D space represents a grand challenge in both soft matter science and biomanufacturing. Here, we present a voxelated bioprinting technology that enables the digital assembly of interpenetrating alginate and polyacrylamide (PAM) double-network (DN) hydrogel droplets. The hydrogel is crosslinked via additive-free bioorthogonal chemistry involving a pair of stoichiometrically matched polymers. We develop theoretical frameworks to describe the crosslinking kinetics and stiffness of the hydrogels, and construct a diagram-of-state to delineate their mechanical properties. Multi-channel print nozzles are developed to allow on-demand mixing of highly viscoelastic bio-inks without significantly impairing cell viability. Further, we showcase the distinctive capability of voxelated bioprinting by creating highly complex 3D structures such as a hollow sphere composed of interconnected yet distinguishable hydrogel particles. Finally, we validate the cytocompatibility andin vivostability of the printed DN scaffolds through cell encapsulation and animal transplantation.
AgRP neuron activity promotes associations between sensory and nutritive signals to guide flavor preference
Authors: Nyema Nathaniel T. , McKnight Aaron D. , Vargas-Elvira Alexandra G. , Schneps Heather M. , Gold Elizabeth G. , Myers Kevin P. , Alhadeff Amber L. 
Published: September 2023

AbstractObjectiveThe learned associations between sensory cues (e.g., taste, smell) and nutritive value (e.g., calories, post-ingestive signaling) of foods powerfully influences our eating behavior [1], but the neural circuits that mediate these associations are not well understood. Here, we examined the role of agouti-related protein (AgRP)-expressing neurons – neurons which are critical drivers of feeding behavior [2; 3] – in mediating flavor-nutrient learning (FNL).MethodsBecause mice prefer flavors associated with AgRP neuron activity suppression [4], we examined how optogenetic stimulation of AgRP neurons during intake influences FNL, and used fiber photometry to determine how endogenous AgRP neuron activity tracks associations between flavors and nutrients.ResultsWe unexpectedly found that tonic activity in AgRP neurons during FNL potentiated, rather than prevented, the development of flavor preferences. There were notable sex differences in the mechanisms for this potentiation. Specifically, in male mice, AgRP neuron activity increased flavor consumption during FNL training, thereby strengthening the association between flavors and nutrients. In female mice, AgRP neuron activity enhanced flavor-nutrient preferences independently of consumption during training, suggesting that AgRP neuron activity enhances the reward value of the nutrient-paired flavor. Finally,in vivoneural activity analyses demonstrated that acute AgRP neuron dynamics track the association between flavors and nutrients in both sexes.ConclusionsOverall, these data (1) demonstrate that AgRP neuron activity enhances associations between flavors and nutrients in a sex-dependent manner and (2) reveal that AgRP neurons track and update these associations on fast timescales. Taken together, our findings provide new insight into the role of AgRP neurons in assimilating sensory and nutritive signals for food reinforcement.