bluuebunny/crossref_metadata_2025_split huggingface dataset:
Beauveria bassiana interacts with gut and hemocytes to manipulate Aedes aegypti immunity
Authors: de Oliveira Barbosa Bitencourt Ricardo , Corrêa Thaís Almeida , Santos-Mallet Jacenir , Santos Huarrisson Azevedo , Lowenberger Carl , Moreira Haika Victória Sales , Gôlo Patrícia Silva , Bittencourt Vânia Rita Elias Pinheiro , da Costa Angelo Isabele 
Published: January 2023

Abstract
Background
Mosquito-borne diseases affect millions of people. Chemical insecticides are currently employed against mosquitoes. However, many cases of insecticide resistance have been reported. Entomopathogenic fungi (EPF) have demonstrated potential as a bioinsecticide. Here, we assessed the invasion of the EPF Beauveria bassiana into Aedes aegypti larvae and changes in the activity of phenoloxidase (PO) as a proxy for the general activation of the insect innate immune system. In addition, other cellular and humoral responses were evaluated.

Methods
Larvae were exposed to blastospores or conidia of B. bassiana CG 206. After 24 and 48 h, scanning electron microscopy (SEM) was conducted on the larvae. The hemolymph was collected to determine changes in total hemocyte concentration (THC), the dynamics of hemocytes, and to observe hemocyte-fungus interactions. In addition, the larvae were macerated to assess the activity of PO using L-DOPA conversion, and the expression of antimicrobial peptides (AMPs) was measured using quantitative Real-Time PCR.

Results
Propagules invaded mosquitoes through the midgut, and blastopores were detected inside the hemocoel. Both propagules decreased the THC regardless of the time. By 24 h after exposure to conidia the percentage of granulocytes and oenocytoids increased while the prohemocytes decreased. By 48 h, the oenocytoid percentage increased significantly (P < 0.05) in larvae exposed to blastospores; however, the other hemocyte types did not change significantly. Regardless of the time, SEM revealed hemocytes adhering to, and nodulating, blastospores. For the larvae exposed to conidia, these interactions were observed only at 48 h. Irrespective of the propagule, the PO activity increased only at 48 h. At 24 h, cathepsin B was upregulated by infection with conidia, whereas both propagules resulted in a downregulation of cecropin and defensin A. At 48 h, blastospores and conidia increased the expression of defensin A suggesting this may be an essential AMP against EPF.

Conclusion
By 24 h, B. bassiana CG 206 occluded the midgut, reduced THC, did not stimulate PO activity, and downregulated AMP expression in larvae, all of which allowed the fungus to impair the larvae to facilitate infection. Our data reports a complex interplay between Ae. aegypti larvae and B. bassiana CG 206 demonstrating how this fungus can infect, affect, and kill Ae. aegypti larvae.

Graphical Abstract
Estimates and trends of the global burden of NASH-related liver cancer attributable to high fasting plasma glucose in 1990–2019: analysis of data from the 2019 Global Burden of Disease Study
Authors: Li Ziyi , Yang Na , He Liyun , Wang Jialu , Ping Fan , Li Wei , Xu Lingling , Zhang Huabing , Li Yuxiu 
Published: January 2023

AbstractBackgroundExperimental and epidemiological studies have indicated an association between diabetes exposure and an increased risk of liver cancer due to nonalcoholic steatohepatitis (NASH). However, to date, no systematic study has specifically investigated the burden of NASH-related liver cancer due to exposure to high fasting plasma glucose (HFPG) levels worldwide.MethodsThe number and rate of deaths and disability-adjusted life years (DALYs) from HFPG-induced NASH-related liver cancer were estimated based on the results of the 2019 Global Burden of Disease Study. The estimated annual percentage changes (EAPCs) for age-standardized death or DALYs rates were calculated using a generalized linear model with a Gaussian distribution to quantify the temporal trends in the global burden of NASH-related liver cancer attributable to HFPG. The strength and direction of the association between the sociodemographic index (SDI) and death or DALY rate were measured using Spearman’s rank-order correlation.ResultsGlobally, approximately 7.59% of all DALY and 8.76% of all mortalities of NASH-related liver cancer in 2019 were due to HFPG. The age-standardized death and DALY rates of NASH-related liver cancer attributable to HFPG increased from 1990 to 2019. The corresponding EAPCs were 0.69 (95% UI 0.48–0.89), and 0.30 (95% UI 0.05–0.56), respectively. This increasing pattern was most obvious in the high- and low-SDI regions. The age-standardized mortality and DALYs rate of NASH-related liver cancer attributable to HFPG varies considerably worldwide, with the middle SDI region having the highest death and DALY rates in 2019 (DALY 0.96 [95% UI 0.23–2.18]; death 0.05 [95% UI 0.01–0.11]).ConclusionThe burden of NASH-related liver cancer attributable to HFPG has increased over the past three decades, particularly in regions with high and low SDI.
Geographical discrepancy in oral food challenge utilization based on Canadian billing data
Authors: El Baba Ala , Jeimy Samira , Soller Lianne , Kim Harold , Begin Philippe , Chan Edmond S. 
Published: January 2023

Abstract
Background
Oral food challenges (OFC) confer the highest sensitivity and specificity in diagnosis; however, uptake has been variable across clinical settings. Numerous barriers were identified in literature from inadequate training to resource access. OFC utilization patterns using billing data have not been previously studied.

Objective
The objective of this study is to explore the geographic differences in utilization of OFCs across Ontario and Québec using anonymized billing data from 2013 to 2017.

Methods
Anonymized OFC billing data were obtained between 2013 and 2017 from Ontario Health Insurance Plan (OHIP) and Régie de l'Assurance Maladie du Québec (RAMQ). The number of OFCs was extracted by location, billings, and physician demographics for clinic and hospital-based challenges.

Results
Over the period studied, the number of OFCs increased by 92% and 85% in Ontario clinics and Québec hospitals, respectively. For Ontario hospitals, the number of OFCs increased by 194%. While Québec performed exclusively hospital-based OFCs, after controlling for the population, the number of OFCs per 100,000 residents annually were similar to Ontario at 50 and 49 OFCs, respectively. The number of OFCs varied across the regions studied with an annual rate reaching up to 156 OFCs per 100,000 residents in urban regions and as low as 0.1 in regions furthest from city centers.

Conclusion
OFC utilization has steadily increased over the last decade. There has been marked geographical discrepancies in OFC utilization which could be driven by the location of allergists and heterogeneity in their practices. More research is needed to identify barriers and propose solutions to them.
Treatment with pollen allergen immunotherapy improves health-related quality of life in children and adolescents: a three-year follow-up-study
Authors: Agenäs Helena , Brorsson Anna Lena , Kull Inger , Lindholm-Olinder Anna 
Published: January 2023

Abstract
Background
The immunological effect of allergen-specific immunotherapy is well documented, but few studies have examined the long-term effects of pollen subcutaneous immunotherapy (SCIT) on health-related quality of life (HRQoL) in children and adolescents. Therefore, the aims of this study were to evaluate the effect of pollen SCIT on HRQoL and to assess the association between HRQoL and symptoms among children and adolescents with allergic rhinoconjunctivitis in a 3-year follow-up.

Methods
A prospective cohort study was conducted at a paediatric clinic in Sweden, including 158 children (5–16 years) on SCIT (birch and/or grass). Health-related quality of life, measured with DISABKIDS, symptom scores and allergen-specific IgE and IgG4 antibodies (blood test), were assessed at start, and after 1, 2 and 3 years of treatment. ANOVA and t-test were used to analyse differences over time, between groups and linear mixed model for the association between HRQoL and influencing factors.

Results
After 1 year of pollen SCIT, HRQoL improved from 79.5 to 85.1 (p < 0.001), and the improvements were maintained (mean 1 years, 84.8, 3 years 87.2). Symptom scores decreased after 1 year, mean 19.9 to 11.5 (p < 0.001) and were maintained for year two (11.9) and year three (10.3). The proportion of children with severe or very severe symptoms decreased from 35.6% to 4.5% after 1 year of SCIT. Health-related quality of life was associated with symptoms at all measured timepoints (p = 0.001–0.031); higher symptom scores were associated with lower perceived HRQoL. Allergen-specific IgE antibodies decreased, birch from 151.0 to 76.8 kU/L (p < 0.001), and IgG4 antibodies increased, birch from 2.2 to 17.6 g/L (p < 0.001), grass from 0.5 to 14.3 g/L (p < 0.001), during the study period.

Conclusion
After 1 year of pollen SCIT, HRQoL improved, and symptoms decreased; these changes were maintained during the study period. The proportion of severe and very severe symptoms significantly decreased.
Cost optimization in cloud environment based on task deadline
Authors: Ahmad Saima Gulzar , Iqbal Tassawar , Munir Ehsan Ullah , Ramzan Naeem 
Published: January 2023

AbstractThe popularity of cloud and fog services has raised the number of users exponentially. Main advantage of Cloud/fog infrastructure and services are crucial specially for commercial users from diverse areas. The variety of service requests with different deadlines makes the task of a service broker challenging. The fog and cloud users always lookfor a suitable compromise between cost and quality of service in terms of response time therefore, the cost optimization is vital for the cloud/fog service providers to capture the market. In this paper an algorithm, Cost Optimization in the cloud/fog environment based on Task Deadline (COTD) is proposed that optimizes cost without compromising the response time. In this algorithm the task deadline is considered as a constraint and an appropriate data center for task processing is selected. The proposed algorithm is suitable for runtime decision making due to its low complexity. The proposed algorithm is evluated using a well-known simulation tool Cloud Analyst. Our comprehensive testbed simulations show that COTD outperforms the existing schemes, Service Proximity Based Routing and Performance-Optimized Routing. The proposed algorithm successfully minimizes the cost by 35% on average while maintaining the response time.
An enhanced ordinal optimization with lower scheduling overhead based novel approach for task scheduling in cloud computing environment
Authors: Yadav Monika , Mishra Atul 
Published: January 2023

AbstractEfficient utilization of available computing resources in Cloud computing is one of the most challenging problems for cloud providers. This requires the design of an efficient and optimal task-scheduling strategy that can play a vital role in the functioning and overall performance of the cloud computing system. Optimal Schedules are specifically needed for scheduling virtual machines in fluctuating & unpredictable dynamic cloud scenario. Although there exist numerous approaches for enhancing task scheduling in the cloud environment, it is still an open issue. The paper focuses on an improved & enhanced ordinal optimization technique to reduce the large search space for optimal scheduling in the minimum time to achieve the goal of minimum makespan. To meet the current requirement of optimal schedule for minimum makespan, ordinal optimization that uses horse race conditions for selection rules is applied in an enhanced reiterative manner to achieve low overhead by smartly allocating the load to the most promising schedule. This proposed ordinal optimization technique and linear regression generate optimal schedules that help achieve minimum makespan. Furthermore, the proposed mathematical equation, derived using linear regression, predicts any future dynamic workload for a minimum makespan period target.
The function and clinical implication of YTHDF1 in the human system development and cancer
Authors: Ren Wenjun , Yuan Yixiao , Li Yongwu , Mutti Luciano , Peng Jun , Jiang Xiulin 
Published: January 2023

AbstractYTHDF1 is a well-characterized m6A reader protein that is essential for protein translation, stem cell self-renewal, and embryonic development. YTHDF1 regulates target gene expression by diverse molecular mechanisms, such as promoting protein translation or modulating the stability of mRNA. The cellular levels of YTHDF1 are precisely regulated by a complicated transcriptional, post-transcriptional, and post-translational network. Very solid evidence supports the pivotal role of YTHDF1 in embryonic development and human cancer progression. In this review, we discuss how YTHDF1 influences both the physiological and pathological biology of the central nervous, reproductive and immune systems. Therefore we focus on some relevant aspects of the regulatory role played by YTHDF1 as gene expression, complex cell networking: stem cell self-renewal, embryonic development, and human cancers progression. We propose that YTHDF1 is a promising future cancer biomarker for detection, progression, and prognosis. Targeting YTHDF1 holds therapeutic potential, as the overexpression of YTHDF1 is associated with tumor resistance to chemotherapy and immunotherapy.
The effect of feature extraction and data sampling on credit card fraud detection
Authors: Salekshahrezaee Zahra , Leevy Joffrey L. , Khoshgoftaar Taghi M. 
Published: January 2023

AbstractTraining a machine learning algorithm on a class-imbalanced dataset can be a difficult task, a process that could prove even more challenging under conditions of high dimensionality. Feature extraction and data sampling are among the most popular preprocessing techniques. Feature extraction is used to derive a richer set of reduced dataset features, while data sampling is used to mitigate class imbalance. In this paper, we investigate these two preprocessing techniques, using a credit card fraud dataset and four ensemble classifiers (Random Forest, CatBoost, LightGBM, and XGBoost). Within the context of feature extraction, thePrincipal Component Analysis(PCA) andConvolutional Autoencoder(CAE) methods are evaluated. With regard to data sampling, theRandom Undersampling(RUS),Synthetic Minority Oversampling Technique(SMOTE), and SMOTE Tomek methods are evaluated. The F1 score andArea Under the Receiver Operating Characteristic Curve(AUC) metrics serve as measures of classification performance. Our results show that the implementation of the RUS method followed by the CAE method leads to the best performance for credit card fraud detection.
Interferon Alfa-2b Adjuvant Therapy of High-Risk Resected Cutaneous Melanoma: The Eastern Cooperative Oncology Group Trial EST 1684
Authors: Kirkwood J M , Strawderman M H , Ernstoff M S , Smith T J , Borden E C , Blum R H 
Published: January 2023

PURPOSE Interferon alfa-2b (IFN alpha-2b) exhibits antitumor activity in metastatic melanoma and on this basis has been evaluated as an adjuvant therapy following surgery for deep primary (T4) or regionally metastatic (N1) melanoma. METHODS A randomized controlled study of IFN alpha-2b (Schering-Plough, Kenilworth, NJ) administered at maximum-tolerated doses of 20 MU/m2/d intravenously (i.v.) for 1 month and 10 MU/m2 three times per week subcutaneously (SC) for 48 weeks versus observation, was conducted by the Eastern Cooperative Oncology Group (ECOG) in 287 patients. RESULTS A significant prolongation of relapse-free survival (P = .0023, one-sided) and prolongation of overall survival (P = .0237, one-sided) was observed with IFN alpha-2b therapy in this trial, which is now mature with a median follow-up time of 6.9 years. The impact of treatment on relapse rate is most pronounced early during the treatment interval. The overall benefit of treatment in this trial was analyzed stratified by tumor burden and the presence or absence of microscopic nonpalpable and palpable regional lymph node metastasis. The benefit of therapy with IFN alpha-2b was greatest among node-positive strata. Toxicity of IFN alpha-2b required dose modification in the majority of patients, but treatment at > or = 80% of the scheduled dose was feasible in the majority of patients through the IV phase of treatment, and for more than 3 months of SC maintenance therapy. Discontinuation of treatment due to toxicity was infrequent after the fourth month of therapy. CONCLUSION IFN alpha-2b prolongs the relapse-free interval and overall survival of high-risk resected melanoma patients. The increment in median disease-free survival (from 1 to 1.7 years) and overall survival (from 2.8 to 3.8 years) that results from this therapy is associated with a 42% improvement in the fraction of patients who are continuously disease-free after treatment with IFN (from 26% to 37%) in comparison to observation. IFN alpha-2b is the first agent to show a significant benefit in relapse-free and overall survival of high-risk melanoma patients in a randomized controlled trial.
Veterans Health Administration National TeleOncology Service
Authors: Zullig Leah L. , Raska Whitney , McWhirter Gina , Sherman Scott E. , Makarov Danil , Becker Daniel , King Heather A. , Pura John , Jeffreys Amy S. , Danus Susanne , Passero Vida , Goldstein Karen M. , Kelley Michael J. 
Published: January 2023

PURPOSE: As the largest integrated health care system in the United States, the Veterans Health Administration (VA) is a leader in telehealth-delivered care. All 10 million Veterans cared for within the VA are eligible for telehealth. The VA cares for approximately 46,000 Veteran patients with newly diagnosed cancer and an estimated 400,000 prevalent cases annually. With nearly 38% of VA health care system users residing in rural areas and only 44% of rural counties having an oncologist, many Veterans lack local access to specialized cancer services. METHODS: We describe the VA's National TeleOncology (NTO) Service. NTO was established to provide Veterans with the opportunity for specialized treatment regardless of geographical location. Designed as a hub-and-spoke model, VA oncologists from across the country can provide care to patients at spoke sites. Spoke sites are smaller and rural VA medical centers that are less able to independently provide the full range of services available at larger facilities. In addition to smaller rural spoke sites, NTO also provides subspecialized oncology care to Veterans located in larger VA medical facilities that do not have subspecialties available or that have limited capacity. RESULTS: As of fiscal year 2021, 23 clinics are served by or engaged in planning for delivery of NTO and there are 24 physicians providing care through the NTO virtual hub. Most NTO physicians continue to provide patient care in separate traditional in-person clinics. Approximately 4,300 unique Veterans have used NTO services. Approximately half (52%) of Veterans using NTO lived in rural areas. Most of these Veterans had more than one remote visit through NTO. CONCLUSION: NTO is a state-of-the-art model that has the potential to revolutionize the way cancer care is delivered, which should improve the experience of Veterans receiving cancer care.
Foreign-Body Reaction Resulting in Thyroiditis—A Complication of Fine-Needle Aspiration Biopsy of the Thyroid
Authors: Adeoti Oluwatomi , Cerda Sandra , Pearce Elizabeth 
Published: January 2023

Abstract
Fine-needle aspiration biopsy (FNAB) is a cost-effective and safe office procedure performed to evaluate thyroid nodules. We report a case of a 33-year-old woman who presented with pain and swelling of the thyroid after undergoing an FNAB for a right thyroid nodule at an outside hospital. The patient received a course of antibiotics without complete resolution of her symptoms and ultimately, underwent a right thyroid lobectomy. Pathology of the excised thyroid tissue showed chronic lymphohistiocytic inflammation with giant cell reaction and hemorrhage consistent with a foreign-body reaction. The patient's symptoms completely resolved after the surgery. Though uncertain what foreign body was introduced into the thyroid tissue during the FNAB, we surmise that an accidental introduction of ultrasound gel may have caused this rare and unexpected complication of a procedure with usually few and manageable complications.
Home ranges, directionality and the influence of moon phases on the movement ecology of Indian flying fox males in southern India
Authors: Murugavel Baheerathan , Kandula Sripathi , Somanathan Hema , Kelber Almut 
Published: January 2023

ABSTRACT
Flying foxes of the genus Pteropus are amongst the largest fruit bats and potential long-range pollinators and seed dispersers in the paleotropics. Pteropus giganteus (currently P. medius) is the only flying fox that is distributed throughout the Indian mainland, including in urban and rural areas. Using GPS telemetry, we mapped the home ranges and examined flight patterns in P. giganteus males across moon phases in a semi-urban landscape in southern India. Home range differed between the tracked males (n=4), likely due to differences in their experience in the landscape. We found that nightly time spent outside the roost, distance commuted and the number of sites visited by tracked individuals did not differ significantly between moon phases. In 61% of total tracked nights across bats, the first foraging site was within 45˚ of the emergence direction. At the colony-level, scan-based observations showed emergence flights were mostly in the northeast (27%), west (22%) and southwest (19%) directions that could potentially be related to the distribution of foraging resources. The movement ecology of fruit bats in relation to the pollination and seed dispersal services they provide requires to be investigated in future studies.
This article has an associated First Person interview with the first author of the paper.
Exploring generic principles of compartmentalization in a developmental in vitro model
Authors: Gires Pierre-Yves , Thampi Mithun , Krauss Sebastian W. , Weiss Matthias 
Published: January 2023

ABSTRACT
Self-organization of cells into higher-order structures is key for multicellular organisms, for example via repetitive replication of template-like founder cells or syncytial energids. Yet, very similar spatial arrangements of cell-like compartments (‘protocells’) are also seen in a minimal model system of Xenopus egg extracts in the absence of template structures and chromatin, with dynamic microtubule assemblies driving the self-organization process. Quantifying geometrical features over time, we show here that protocell patterns are highly organized with a spatial arrangement and coarsening dynamics similar to that of two-dimensional foams but without the long-range ordering expected for hexagonal patterns. These features remain invariant when enforcing smaller protocells by adding taxol, i.e. patterns are dominated by a single, microtubule-derived length scale. Comparing our data to generic models, we conclude that protocell patterns emerge by simultaneous formation of randomly assembling protocells that grow at a uniform rate towards a frustrated arrangement before fusion of adjacent protocells eventually drives coarsening. The similarity of protocell patterns to arrays of energids and cells in developing organisms, but also to epithelial monolayers, suggests generic mechanical cues to drive self-organized space compartmentalization.