bluuebunny/crossref_metadata_2025_split huggingface dataset:
Exploring the Probiotic Proficiency of Dairyderived Lactic Acid Bacteria and Their Antimicrobial Efficacy Against Multi-drug Resistant Diarrheal and Uro-Pathogens
Authors: Abonee Fatema Jannat , Mishu Israt Dilruba , Akter Sarmin , Rahman Sabita Rezwana , Malek Md Abdul 
Published: June 2023

Multidrug resistance (MDR) poses a global health threat, necessitating the exploration of alternative solutions. Probiotics, especially lactic acid bacteria (LAB), offer promising options against the impending crisis due to their recognized safety and potential health benefits. Probiotic potential characterization and selection of candidate LAB strains are highly crucial in probiotic product formulation. This study aimed to identify LAB from dairy product yogurt and evaluate their potential probiotic properties, i.e. aggregation capacity; tolerance to gastric and intestinal conditions; as well as antimicrobial potency. Ten LAB isolates were characterized based on colony characteristics, cellular morphology, and biochemical tests. The LAB isolates, both single and in mixed consortia, displayed a time-dependent increase in auto-aggregation, ranging from 21% to 71% after 5 hours of incubation. Isolate SW2 exhibited the highest auto-aggregative capability (65%). Co-aggregation studies revealed varying degrees of co-aggregation between probiotic LAB and pathogens, with some isolates showing stronger interactions (YD3, SW1, and SW2). Mixed consortia from sample TT demonstrated the highest co-aggregative ability with all tested pathogens. These findings highlight the potential of these isolates to form protective clusters, aiding in their survival and colonization within the gastrointestinal (GI) tract, besides the competitive exclusion of pathogens. The isolates demonstrated good tolerance to simulated gastric and intestinal conditions, as indicated by their non-significant reduction (only 1-2 log) in the bacterial count after 180 minutes of treatment. These findings indicate that LAB isolates can withstand harsh GI conditions, highlighting their suitability as probiotics. Antimicrobial profiles of the LAB isolates were evaluated using radial streak method and turbidimetric microtiter plate assay against eight MDR diarrheal and Uro-pathogens (n=4 for each). LAB isolates SKY1, SW1, SW3 and TT1 exhibited the highest antimicrobial activities; while pathogens DP2, UP41 and UP42 showed the most sensitivity. Exhibited antimicrobial activity of the LAB isolates points to their potential as formidable weapons against MDR infections. Overall, the results indicate that dairy-derived LAB isolates used in this study exhibit potential probiotic traits. Further research is warranted for their mechanisms, safety, efficacy, and use in probiotic supplement development.
Bioresearch Commu. 9(2): 1340-1350, 2023 (July)
Limb Salvage Surgery in Large and Fungating Giant Cell Tumors of Distal Radius: A Case Series
Authors: Budi Muhamad Naseh Sajadi , Abidin Arief 
Published: June 2023

Introduction: A Giant Cell Tumor (GCT) is a benign tumor, but often aggressive with recurrence or metastasis tendency. Often, distal radius GCT is presented in relatively small or medium sizes. Large and fungating distal radius GCT is characterized by its higher ability to infiltrate, destruct, and compromise its surrounding tissue. Therefore, it increases the risk of complications including amputation. The treatment requires more complex surgery and reconstruction than usual GCT. In this case series, we prefer to perform the limb salvage surgery with wide en bloc resection, followed by reconstruction of the bone and soft tissue, instead of amputation. Case Presentation: We report 4 patients with massive GCT at the distal radius. All complained about pain and a lump in the wrist. The tumors are large in dimension with the size of 12 x 8 x 6 cm, 10 x 10 x 8 cm, 11 x 9 x 9 cm, and 17 x 14 x 8 cm respectively, fungating and compromised surrounding soft tissue. Limb salvage procedures are performed with wide en-bloc resection, bone cement spacer insertion, K-wire fixation, dissection, and tendon reconstructions followed by soft tissue defect closure using distant flap and primary suture. Follow-up post-surgery was variable within 2 to 36 months. All patients had a reduced scale of VAS, increased functional outcome measured with quick DASH score, no major complications, and no sign of recurrence or metastasis during the follow-up period. Conclusion: Large and fungating GCT of distal radius managed by limb salvage surgery and complex reconstruction deliver a good result. Thus, it can give other options other than amputation.
Perencanaan Plumbing Instalasi Air Bersih Pada Gedung Kantor Perwakilan Bank Indonesia Di Kota Kendari Sulawesi Tenggara
Authors: Rahmawati Ikra , Rachman Ranno Marlany , Sarita Umran , Arsyad La Ode Muhamad Nurrakhmad , Talanipa Romy 
Published: June 2023

Bangunan Gedung Kantor Perwakilan Bank Indonesia di Kota Kendari Sulawesi Tenggara yang dibangun pada tanggal 23 November 2018 yang terdiri dari 4 lantai dan mempunyai luas ± 1.920 m2 perlantainya. Demi mendukung kapasitas serta fungsinya, maka persediaan air dengan kualitas dan kuantitas yang baik, pembuangan air kotor dan air hujan yang tidak ada hambatan, serta sistem pencegah kebakaran yang memadai mutlak di perlukan. Penelitian ini bertujuan untuk mengetahui jumlah kebutuhan air bersih yang diperlukan penghuni Gedung Kantor Perwakilan Bank Indonesia di Kota Kendari Sulawesi Tenggara yang dihitung dengan menggunakan tiga metode yaitu berdasarkan jumlah penghuni, jenis dan jumlah alat plumbing, berdasarkan unit beban alat plumbing dengan menggunakan data prime dan sekunder. Hasil penelitian ini menunjukkan bahwa kebutuhan air bersih pada Gedung Kantor Perwakilan Bank Indonesia berdasarkan jumlah penghuni rata- rata perhari sebesar 53,8 m3/hari. Perhitungan kebutuhan air bersih berdasarkan jumlah alat plumbing pada gedung kantor pemakaian air perhari sebesar 303,4 m3/hari. Perhitungan kebutuhan air bersih berdasarkan unit beban alat plumbing pada gedung kantor pemakaian air perhari sebesar 69 m3/hari.
MENINGKATKAN KEAKTIFAN DAN HASIL BELAJAR MATA PELAJARAN IPS MELALUI PENERAPAN MODEL PEMBELAJARAN MASTERY LEARNING
Authors: Uswah Alia 
Published: June 2023

Based on the analysis of daily repeat results or midterm and semester repeats, it is known that the learning outcomes of students in MTs.N 3 Rembang Class VIII for the 2019/2020 school year in IPS subjects are low.Â This is shown by the following facts: Learners who get grades below KKM there are 50%, students who get the same value as KKM there are 35% and students who get a score above KKM there is 15%. It is expected that from the learning carried out is the learning outcome of learners in IPS subjects at least 30% of learners achieve grades above KKM, 50% of learners achieve the same value as KKM, and 20% of learners achieve grades below KKM. This research includes a type of classroom action research (PTK), with its main characteristic being repeated actions and the main method is self-reflection aimed at improving learning. PTK is a problem-solving activity characterized by cyclic and reflection that starts from: a) planning, b) actions, c) observing, d) analyzing data/information to decide the extent of the advantages and disadvantages of such actions (reflecting). PTK is characterized by continuous improvement so that research satisfaction is often the benchmark of the cycle. From the analysis of the causes of the problem, the efforts that are expected to improve the learning outcomes of Class VIII learners in IPS subjects are the application of the right model, namely a learning model that allows students to conduct exercises, discussions, direct guidance of teachers until learners can solve IPS subject problems. The model that is suspected to be right is the Mastery Learning model. The steps of this model are (1) Orientation, (2) Presentation, (3) Structured exercises, (4) Guided exercises, (5) and Self-Training. This class action research is to find out the increase in the activeness and learning outcomes of class VIII MTs Negeri 3 Rembang odd students in 2019/2020 in the IPS through the application of the Mastery Learning model.
Relationships between quantitative retinal microvascular characteristics and cognitive function based on automated artificial intelligence measurements
Authors: Shi Xu Han , Dong Li , Zhang Rui Heng , Zhou Deng Ji , Ling Sai Guang , Shao Lei , Yan Yan Ni , Wang Ya Xing , Wei Wen Bin 
Published: June 2023

Introduction: The purpose of this study is to assess the relationship between retinal vascular characteristics and cognitive function using artificial intelligence techniques to obtain fully automated quantitative measurements of retinal vascular morphological parameters.Methods: A deep learning-based semantic segmentation network ResNet101-UNet was used to construct a vascular segmentation model for fully automated quantitative measurement of retinal vascular parameters on fundus photographs. Retinal photographs centered on the optic disc of 3107 participants (aged 50–93 years) from the Beijing Eye Study 2011, a population-based cross-sectional study, were analyzed. The main parameters included the retinal vascular branching angle, vascular fractal dimension, vascular diameter, vascular tortuosity, and vascular density. Cognitive function was assessed using the Mini-Mental State Examination (MMSE).Results: The results showed that the mean MMSE score was 26.34 ± 3.64 (median: 27; range: 2–30). Among the participants, 414 (13.3%) were classified as having cognitive impairment (MMSE score &lt; 24), 296 (9.5%) were classified as mild cognitive impairment (MMSE: 19–23), 98 (3.2%) were classified as moderate cognitive impairment (MMSE: 10–18), and 20 (0.6%) were classified as severe cognitive impairment (MMSE &lt; 10). Compared with the normal cognitive function group, the retinal venular average diameter was significantly larger (p = 0.013), and the retinal vascular fractal dimension and vascular density were significantly smaller (both p &lt; 0.001) in the mild cognitive impairment group. The retinal arteriole-to-venular ratio (p = 0.003) and vascular fractal dimension (p = 0.033) were significantly decreased in the severe cognitive impairment group compared to the mild cognitive impairment group. In the multivariate analysis, better cognition (i.e., higher MMSE score) was significantly associated with higher retinal vascular fractal dimension (b = 0.134, p = 0.043) and higher retinal vascular density (b = 0.152, p = 0.023) after adjustment for age, best corrected visual acuity (BCVA) (logMAR) and education level.Discussion: In conclusion, our findings derived from an artificial intelligence-based fully automated retinal vascular parameter measurement method showed that several retinal vascular morphological parameters were correlated with cognitive impairment. The decrease in retinal vascular fractal dimension and decreased vascular density may serve as candidate biomarkers for early identification of cognitive impairment. The observed reduction in the retinal arteriole-to-venular ratio occurs in the late stages of cognitive impairment.
The role of retinal vessel geometry as an indicator of systemic arterial stiffness assessed by cardio-ankle vascular index
Authors: Ma Dae Joong , Lee Heesun , Choi Ji Min , Park Hyo Eun , Choi Su-Yeon , Choi Hyuk Jin 
Published: June 2023

ObjectiveTo determine whether retinal vessel geometry is associated with systemic arterial stiffness, as determined by the cardio-ankle vascular index (CAVI).MethodsThis single-center retrospective cross-sectional study included 407 eyes of 407 subjects who underwent routine health exams, including CAVI and fundus photography. Retinal vessel geometry was measured using a computer-assisted program (Singapore “I” Vessel Assessment). Subjects were classified into two groups based on CAVI values: high CAVI (≥9) or low CAVI (&lt;9). The main outcome measures included the association of retinal vessel geometry and CAVI value evaluated using multivariable logistic regression models.ResultsThree hundred forty-three subjects (343, 84.3%) were in the low CAVI group, and 64 (15.7%) subjects were in the high CAVI group. Multivariable logistic linear regression analyses adjusted for age, sex, body mass index, smoking status, mean arterial pressure, and the presence of hypertension, diabetes mellitus, and dyslipidemia showed a significant association between high CAVI values and the following retinal vessel geometry parameters: central retinal arteriolar equivalent caliber (CRAE; adjusted odds ratio [AOR], 0.95; 95% confidence interval [CI], 0.89–1.00; P = 0.043), fractal dimension of arteriolar network (FDa; AOR, 4.21 × 10−4; 95% CI, 2.32 × 10−7−0.77; P = 0.042), and arteriolar branching angle (BAa; AOR, 0.96; 95% CI, 0.93–0.99; P = 0.007).ConclusionsIncreased systemic arterial stiffness had a significant association with retinal vessel geometry related to arterial narrowing (CRAE), less branching complexity of the arterial tree (FDa), and acute arteriolar bifurcation (BAa).
Clinical outcomes and predictors of success with Impella weaning in cardiogenic shock: a single-center experience
Authors: Matassini M. V. , Marini M. , Angelozzi A. , Angelini L. , Shkoza M. , Compagnucci P. , Falanga U. , Battistoni I. , Pongetti G. , Francioni M. , Piva T. , Mucaj A. , Nicolini E. , Maolo A. , Di Eusanio M. , Munch C. , Dello Russo A. , Perna G. 
Published: June 2023

IntroductionCardiogenic shock (CS) is a severe syndrome with poor prognosis. Short-term mechanical circulatory support with Impella devices has emerged as an increasingly therapeutic option, unloading the failing left ventricle (LV) and improving hemodynamic status of affected patients. Impella devices should be used for the shortest time necessary to allow LV recovery because of time-dependent device-related adverse events. The weaning from Impella, however, is mostly performed in the absence of established guidelines, mainly based on the experience of the individual centres.MethodsThe aim of this single center study was to retrospectively evaluate whether a multiparametrical assessment before and during Impella weaning could predict successful weaning. The primary study outcome was death occurring during Impella weaning and secondary endpoints included assessment of in-hospital outcomes.ResultsOf a total of 45 patients (median age, 60 [51–66] years, 73% male) treated with an Impella device, 37 patients underwent impella weaning/removal and 9 patients (20%) died after the weaning. Non-survivors patients after impella weaning more commonly had a previous history of known heart failure (p = 0.054) and an implanted ICD-CRT (p = 0.01), and were more frequently treated with continuous renal replacement therapy (p = 0.02). In univariable logistic regression analysis, lactates variation (%) during the first 12–24 h of weaning, lactate value after 24 h of weaning, left ventricular ejection fraction (LVEF) at the beginning of weaning, and inotropic score after 24 h from weaning beginning were associated with death. Stepwise multivariable logistic regression identified LVEF at the beginning of weaning and lactates variation (%) in the first 12–24 h from weaning beginning as the most accurate predictors of death after weaning. The ROC analysis indicated 80% accuracy (95% confidence interval = 64%–96%) using the two variables in combination to predict death after weaning from Impella.ConclusionsThis single-center experience on Impella weaning in CS showed that two easily accessible parameters as LVEF at the beginning of weaning and lactates variation (%) in the first 12–24 h from weaning begin were the most accurate predictors of death after weaning.
Hydrogeochemical characteristic and recognization of saline groundwater formation and evolution in silty coast of the Yellow Sea and Bohai Sea, Eastern China
Authors: Gao Maosheng , Sun Qiming , Dang Xianzhang , Hou Guohua , Guo Fei , Liu Zhenlin , Chang Xinyue , Zhao Guangming 
Published: June 2023

Saline groundwater is a valuable unconventional water resource in silty coastal zone, which contain complex and fragile sedimentary and hydrological environments that are widely distributed and vulnerable to human activities. Coastal groundwater salinization along the eastern coast of China since the late Pleistocene is thought to be a response to global environmental change. Previous studies have shown a spatial and temporal link between the development of saline groundwater and deposition of marine strata. However, groundwater salinization is becoming an increasing problem in coastal zone, and the hydrogeochemical characteristics and cause of the groundwater salinization or desalinization remain poorly understood. In this study, we compared the hydrogeochemical characteristics and relationships between saline groundwater and the sedimentary environment in two representative, muddy coastal zone of the Yellow Sea coast (YSC) and Bohai Sea coast (BSC). Our monitoring results show that the groundwater varies from fresh water, to brackish water, saltwater, and brine moving offshore of the BSC, with total dissolved solid (TDS) concentrations of 203–184,196 mg/L. In contrast, brine is absent from the YSC, where the TDS concentrations range from 280 to 41,690 mg/L. The relationships between δ2H and δ18O values indicate that freshwater–seawater–brine and freshwater–seawater mixing are the main processes leading to enrichment in TDS in the BSC and YSC, respectively. Based on the relationship between the major cations, Cl−, and Cl/Br ratios, the results show that the groundwater salinity is primarily due to saltwater intrusion and secondly to water–rock interactions (i.e., evaporitic salt dissolution and cation exchange). The regression curves on plots of Na–Cl and (Ca+Mg)–(CO3+SO4) have negative slopes (−0.33 and −0.34, respectively) for the groundwater samples from the BSC and YSC, indicating the Na in groundwater exchange for Ca due to seawater or residual seawater intrusion. The saline groundwater (or brine) samples with reverse ion exchange indicate that seawater or residual seawater has invaded the aquifer, and in which Na+ is replacing Ca2+ and Mg2+. These results enhance our understanding of the formation and evolution of saline groundwater, and provide insights into groundwaters in other silty coastal zones.
Association between diabetes status and breast cancer in US adults: findings from the US National Health and Nutrition Examination Survey
Authors: Sun Xingyu , Zhang Qiangsong , Kadier Kaisaierjiang , Hu Pengcheng , Liu Xiaozhu , Liu Jialing , Yan Yulu , Sun Chenyu , Yau Vicky , Lowe Scott , Meng Muzi , Liu Ziru , Zhou Meirong 
Published: June 2023

ObjectivesThe aim of this study was to investigate the association between diabetes status and the risk of breast cancer among adult Americans, exploring the impact of BMI, age, and race on this relationship.MethodsA cross-sectional analysis of 8,249 individuals from the National Health and Nutrition Examination Survey (NHANES) was conducted. Diabetes was categorized as type 2 diabetes and prediabetes, with both conditions diagnosed according to the ADA 2014 guidelines. The association between diabetes status and breast cancer risk was explored using multiple logistic regression analysis.ResultsPatients with diabetes had higher odds of breast cancer (OR: 1.51; 95% CI 1.00 to 2.28), Using the two-piecewise linear regression model, it was observed that there is a threshold effect in the risk of breast cancer occurrence at the age of 52 years. Specifically, the risk of breast cancer is relatively low before the age of 52 but increases significantly after this age.ConclusionsThis study identified a significant association between diabetes status and breast cancer risk among adult Americans. We also found a threshold effect in breast cancer occurrence at the age of 52. Age was significantly associated with breast cancer risk in both Non-Hispanic White and Non-Hispanic Black individuals. These findings underscore the importance of diabetes management, maintaining a healthy BMI, and age-related risk considerations in reducing breast cancer risk.
Impaired mitochondrial dynamics and removal of the damaged mitochondria in diabetic retinopathy
Authors: Alka Kumari , Kumar Jay , Kowluru Renu A. 
Published: June 2023

IntroductionMitochondrial dynamic plays a major role in their quality control, and the damaged mitochondrial components are removed by autophagy. In diabetic retinopathy, mitochondrial fusion enzyme, mitofusin 2 (Mfn2), is downregulated and mitochondrial dynamic is disturbed resulting in depolarized and dysfunctional mitochondria. Our aim was to investigate the mechanism of inhibition of Mfn2, and its role in the removal of the damaged mitochondria, in diabetic retinopathy.MethodsUsing human retinal endothelial cells, effect of high glucose (20mM) on the GTPase activity of Mfn2 and its acetylation were determined. Role of Mfn2 in the removal of the damaged mitochondria was confirmed by regulating its acetylation, or by Mfn2 overexpression, on autophagosomes- autolysosomes formation and the mitophagy flux.ResultsHigh glucose inhibited GTPase activity and increased acetylation of Mfn2. Inhibition of acetylation, or Mfn2 overexpression, attenuated decrease in GTPase activity and mitochondrial fragmentation, and increased the removal of the damaged mitochondria. Similar phenomenon was observed in diabetic mice; overexpression of sirtuin 1 (a deacetylase) ameliorated diabetes-induced inhibition of retinal Mfn2 and facilitated the removal of the damaged mitochondria.ConclusionsAcetylation of Mfn2 has dual roles in mitochondrial homeostasis in diabetic retinopathy, it inhibits GTPase activity of Mfn2 and increases mitochondrial fragmentation, and also impairs removal of the damaged mitochondria. Thus, protecting Mfn2 activity should maintain mitochondrial homeostasis and inhibit the development/progression of diabetic retinopathy.