bluuebunny/crossref_metadata_2025_split huggingface dataset:
ccl19 and ccl21 affect cell movements and differentiation in early Xenopus development
Authors: Goto Toshiyasu , Michiue Tatsuo , Shibuya Hiroshi 
Published: March 2023

AbstractWe characterized Xenopus laevis C‐C motif chemokine ligand 19.L (ccl19.L) and C‐C motif chemokine ligand 21.L (ccl21.L) during early Xenopus embryogenesis. The temporal and spatial expression patterns of ccl19.L and ccl21.L tended to show an inverse correlation, except that the expression level was higher in the dorsal side at the gastrula stage. For example, even at the dorsal sector of the gastrulae, ccl19.L was expressed in the axial region and ccl21.L was expressed in the paraxial region. Dorsal overexpression of ccl19.L and ccl21.L and knockdown of Ccl19.L and Ccl21.L inhibited gastrulation, but their functions were different in cell behaviors during morphogenesis. Observation of Keller sandwich explants revealed that overexpression of both ccl19.L and ccl21.L and knockdown of Ccl21.L inhibited the convergent extension movements, while knockdown of Ccl19.L did not. ccl19.L‐overexpressing explants attracted cells at a distance and ccl21.L‐overexpressing explants attracted neighboring cells. Ventral overexpression of ccl19.L and ccl21.L induced secondary axis‐like structures and chrd.1 expression at the ventral side. Upregulation of chrd.1 was induced by ligand mRNAs through ccr7.S. Knockdown of Ccl19.L and Ccl21.L inhibited gastrulation and downregulated chrd.1 expression at the dorsal side. The collective findings indicate that ccl19.L and ccl21.L might play important roles in morphogenesis and dorsal–ventral patterning during early embryogenesis in Xenopus.
Temporal textures: Time, meaning, and the good life
Authors: Weber‐Guskar Eva 
Published: March 2023

AbstractIn the debate on meaning in life as part of a good human life, the role of time still needs to be worked out in greater detail. This paper argues that making the role of time in a specific sense explicit allows for the development of an account that leaves behind some of the objections with which current accounts are confronted. To show this, I will reconstruct two accounts of meaning in life and critically discuss them—the account of meaning by valuable activities and the account of meaning by intelligibility. The first one struggles with how to distinguish the value of meaning from the values of happiness and morality and neglects the common understanding of meaning in other contexts; the second one at least partly falls back into the older discussion about the meaning of life with undesired theoretical consequences. A new account, called the account of meaning by temporal textures, focuses on narrative intelligibility, showing that meaning in life builds on temporal relations between events. This time focused narrative account avoids some misleading associations and enables one to answer some pertinent questions concerning the relevance of meaning for the good life as a whole.
From basic sciences and engineering to epileptology: A translational approach
Authors: Bou Assi Elie , Schindler Kaspar , de Bézenac Christophe , Denison Timothy , Desai Sharanya , Keller Simon S. , Lemoine Émile , Rahimi Abbas , Shoaran Mahsa , Rummel Christian 
Published: March 2023

AbstractCollaborative efforts between basic scientists, engineers, and clinicians are enabling translational epileptology. In this article, we summarize the recent advances presented at the International Conference for Technology and Analysis of Seizures (ICTALS 2022): (1) novel developments of structural magnetic resonance imaging; (2) latest electroencephalography signal‐processing applications; (3) big data for the development of clinical tools; (4) the emerging field of hyperdimensional computing; (5) the new generation of artificial intelligence (AI)–enabled neuroprostheses; and (6) the use of collaborative platforms to facilitate epilepsy research translation. We highlight the promise of AI reported in recent investigations and the need for multicenter data‐sharing initiatives.
Geographical approaches to religion in the past
Authors: Slatter Ruth 
Published: March 2023

AbstractThis review assesses (anglophone) cross‐disciplinary research that has used geographical methodologies to study religion in the past. It identifies three prominent themes within the existing literature: the spatalisation of religion, the intersections between religion and built environments, and the relationships between religion and physical landscapes. It argues that the application of geographical approaches to the study of religion in the past has made important contributions to feminist and postcolonial attempts to de‐centre religious leaders and social elites. However, it also demonstrates that the existing literature has been fundamentally informed by inherently modern and western definitions of religion. Primarily, it identifies how the existing literature has prioritised the study of institutionalised Abrahamic religions, emphasised the analysis of sacred‐secular dichotomies, and assumed that religious affiliation involves personal belief and spiritual encounter. In response, this paper calls for geographical approaches to religion in the past to engage with a more diverse range of subjects and use network or assemblage approaches to challenge modern and western assumptions about religious practices and experiences.
A simple, rapid, and efficient method for generating multigene‐knockout culture cells by the CRISPR/Cas9 system
Authors: Fujii Toshihiro , Inoue Naomi , Nobeyama Takanobu , Inoue Junko , Ogasawara Shin , Otani Yuhei , Fujii Shinobu , Ito Chihiro , Fukunaga Rikiro 
Published: March 2023

AbstractWe evaluated the efficacy of simultaneous multiple‐gene knockout in human culture cells. By simple co‐transfection of HeLa cells with a mixture of pX330‐based targeting plasmids together with a puromycin resistance plasmid, followed by transient selection of puromycin‐resistant cells, Cas9/single‐guide RNA (sgRNA)‐transduced polyclonal cell populations were selected and grown. Western blot analyses revealed co‐transfection of up to seven targeting plasmids for p38α, p38β, JNK1, JNK2, Mnk1, ERK1, and mLST8 genes, drastically reduced protein expression of these genes in the polyclonal population. Analyses of a randomly isolated group of 25 clones revealed knockout efficiencies for the seven targeted genes ranging between 68% and 100%, and in six clones (24%), all targeted genes were disrupted. Deep sequencing analyses of the individual target sites revealed that, in most cases, Cas9/sgRNA‐induced nonhomologous end joining resulted in deletion or insertion of only a few base pairs at the break points. These results demonstrate that simple co‐transfection‐based simultaneous targeting offers an easy, rapid, and efficient method to generate multiplex gene‐knockout cell lines.
Emergency department utilization among patients who receive outpatient specialty care for headache: A retrospective cohort study analysis
Authors: Phelan Michael P. , Thompson Nicolas R. , Ahmed Zubair , Lapin Brittany , Udeh Belinda , Anderson Eric , Katzan Irene , Walker Laura E. 
Published: March 2023

AbstractObjectivesTo compare clinical characteristics among outpatient headache clinic patients who do and do not self‐report visiting the emergency department for headache.BackgroundHeadache is the fourth most common reason for emergency department visits, compromising 1%–3% of visits. Limited data exist about patients who are seen in an outpatient headache clinic but still opt to frequent the emergency department. Clinical characteristics may differ between patients who self‐report emergency department use and those who do not. Understanding these differences may help identify which patients are at greatest risk for emergency department overutilization.MethodsThis observational cohort study included adults treated at the Cleveland Clinic Headache Center between October 12, 2015 and September 11, 2019, who completed self‐reported questionnaires. Associations between self‐reported emergency department utilization and demographics, clinical characteristics, and patient‐reported outcome measures (PROMs: Headache Impact Test [HIT‐6], headache days per month, current headache/face pain, Patient Health Questionnaire‐9 [PHQ‐9], Patient‐Reported Outcomes Measurement Information System [PROMIS] Global Health [GH]) were evaluated.ResultsOf the 10,073 patients (mean age 44.7 ± 14.9, 78.1% [7872/10,073] female, 80.3% [8087/10,073] White patients) included in the study, 34.5% (3478/10,073) reported visiting the emergency department at least once during the study period. Characteristics significantly associated with self‐reported emergency department utilization included younger age (odds ratio = 0.81 [95% CI = 0.78–0.85] per decade), Black patients (vs. White patients) (1.47 [1.26–1.71]), Medicaid (vs. private insurance) (1.50 [1.29–1.74]), and worse area deprivation index (1.04 [1.02–1.07]). Additionally, worse PROMs were associated with greater odds of emergency department utilization: higher (worse) HIT‐6 (1.35 [1.30–1.41] per 5‐point increase), higher (worse) PHQ‐9 (1.14 [1.09–1.20] per 5‐point increase), and lower (worse) PROMIS‐GH Physical Health T‐scores (0.93 [0.88–0.97]) per 5‐point increase.ConclusionOur study identified several characteristics associated with self‐reported emergency department utilization for headache. Worse PROM scores may be helpful in identifying which patients are at greater risk for utilizing the emergency department.
Understanding the emergence of females as leaders in academia: The intersections of gender stereotypes, status and emotion
Authors: Gaus Nurdiana , Larada Nasrullah , Jamaluddin Syakir , Paramma Muhammad Azwar , Karim Abdul 
Published: March 2023

AbstractDrawing on the expectation state theory, this paper seeks to analyse the social cognitive process of the impacts of gender stereotypes along with their culturally derived schemas of status belief, status characteristics and emotion at the early stage of women's endeavour to emerge as leaders in academia. Employing a convenience sampling and interviews held with five women academic participants from three public and private universities in the western region of Indonesia, this research reveals that two social cognitive practices affect women's endeavour to emerge as leaders; (1) the incongruities of cultural and cognitive expected status belief and status characteristics about females with the expected performance of leadership. The pervasive effect of these can be mitigated when women adopt a strategy of neglection, coupled with a strategy of networking both via their own networks and their husband's networks; (2) the incongruities of cultural and cognitive expected ways of emotional expression on women with the expected performance of leadership. This impacts the status conferral that shapes the worthiness of females to emerge as leaders, leading female leaders in our study to build a protective shield of emotion display to keep them perceived as worthy individuals for leadership roles.
Mesonephric‐like adenocarcinoma of the female genital tract: novel observations and detailed molecular characterisation of mixed tumours and mesonephric‐like carcinosarcomas
Authors: Mirkovic Jelena , Olkhov‐Mitsel Ekaterina , Amemiya Yutaka , Al‐Hussaini Maysa , Nofech‐Mozes Sharon , Djordjevic Bojana , Kupets Rachel , Seth Arun , McCluggage W Glenn 
Published: March 2023

AimsTo report novel observations in five mesonephric‐like adenocarcinomas (MLAs) of the female genital tract.Methods and resultsWe report two endometrial MLAs in association with endometrioid carcinoma and atypical hyperplasia and three (one endometrial, two ovarian) cases with a sarcomatoid component (mesonephric‐like carcinosarcoma). Pathogenic KRAS mutations, which are characteristic of MLA, were identified in all cases although interestingly, in one of the mixed carcinomas, this was confined to the endometrioid component. The concurrent MLA, endometrioid carcinoma and atypical hyperplasia components in one case harboured identical EGFR, PTEN and CCNE1 mutations, suggesting that the atypical hyperplasia gave rise to a Müllerian carcinoma with both endometrioid and mesonephric‐like components. The carcinosarcomas all contained a component of MLA and a sarcomatous component with chondroid elements. In the ovarian carcinosarcomas, the coexisting epithelial and sarcomatous components shared some mutations including KRAS and CREBBP, suggesting that they are clonally related. Furthermore, in one case CREBBP and KRAS mutations detected in the MLA and sarcomatous components were also detected in an associated undifferentiated carcinoma component, suggesting that it was clonally related to the MLA and sarcomatous components.ConclusionsOur observations provide additional evidence that MLAs have a Müllerian origin and characterise mesonephric‐like carcinosarcomas in which chondroid elements appear to be characteristic. In reporting these findings, we provide recommendations for distinction between a mesonephric‐like carcinosarcoma and a MLA with a spindle cell component.
A method for resolving organisation‐enterprise system misfits: An action research study in a pluralistic organisation
Authors: Morquin David , Ologeanu‐Taddei Roxana , Paré Guy , Wagner Gerit 
Published: March 2023

AbstractAlthough off‐the‐shelf enterprise systems (ES) have been widely adopted in organisations, the extant literature repeatedly documents ES failures caused by misfits between organisational processes and the ES. Although some misfits can be identified early in the ES lifecycle, others emerge in the onward and upward phase (i.e., after the implementation) and, hence, must be resolved reactively. Prior research on misfits and resolution strategies has primarily focused on the implementation phase, often assuming that close‐to‐perfect information on the misfit's nature and characteristics is available. However, no study has examined how to effectively complete a shared diagnosis and resolution of misfits when diverging individual user perceptions are taken as the starting point. Such situations may be particularly pronounced in pluralistic organisations, where a variety of interdependent processes and potentially competing perceptions of processes are prevalent. The main objective of this study is to address this gap. To this end, we propose a pragmatic method for the diagnosis and resolution of misfits between organisational processes and enterprise systems, which builds on an actionable conceptualization of misfits. This method builds on theoretical concepts of affordances, affordance actualization, user participation, and change agentry. To demonstrate the feasibility and effectiveness of the proposed method, we conducted an action research study in a university hospital. Our analysis focused on a specific misfit involving the hospital's ES‐supported clinical processes. The findings suggest that the method effectively diagnoses and resolves misfits and optimises the resources required for their resolution through efficient management of user participation. We conclude with a discussion of the theoretical and practical contributions of our work.
Barriers to nurses performing physical assessments in rehabilitation care units: An observational study
Authors: Da Veiga Gonçalves Lorena , Latanioti Maria , Latif Sabah , Verloo Henk , Pereira Filipa 
Published: March 2023

AbstractAimsTo describe the perceived barriers to nurses performing physical assessments of patients in rehabilitation wards. Secondarily, to investigate how sociodemographic and professional characteristics influence the use and frequency of physical assessments by nurses and their perceptions of barriers to their practice.DesignA multicentre, cross‐sectional, observational study.MethodsData were collected from September to November 2020 among nurses working with inpatients in eight rehabilitation care institutions in French‐speaking Switzerland. Instruments included the Barriers to Nurses' use of Physical Assessment Scale.ResultsAlmost half of the 112 nurses who responded reported performing physical assessments regularly. The predominant perceived barriers to performing physical assessments were ‘specialty area’, ‘lack of nursing role models’ and ‘lack of time and interruptions’. Greater clinical nursing experience in rehabilitation wards and more senior nurse specialist positions were associated with significantly lower use of physical assessment procedures by nurses.ConclusionThe present study revealed heterogeneity in the use of physical assessment by nurses practicing in rehabilitation units and highlighted their perceived barriers to this.Impact statementMost nurses working in rehabilitation care units did not routinely perform physical assessments as part of their daily clinical practice. These results should raise stakeholders' awareness of this fact. Effective interventions to increase the use of physical assessments in nursing practice are to be recommended, including continuing education or hiring enough highly qualified nurses as role models in wards. This will promote quality of care and patient safety in rehabilitation care units.Public and patient engagement and involvementThere was no patient or public involvement in the present study.
Fleas and lesions in armadillo osteoderms
Authors: Boyde Alan , Mills David , Abba Agustin Manuel , Ezquiaga María Cecilia 
Published: March 2023

AbstractArmadillos are bitten by several species of flea. Females of the genus Tunga penetrate the epidermis and when in place are fertilised by males, after which the abdomen swells enormously to form a ‘neosome’. Within the penetrans group, T. perforans, makes lesions that perforate the osteoderms within the integument to form ~3 mm diameter cavities occupied by a discoid neosome. We examined these lesions in carapace material from animals which had died in the wild to see whether we could recruit evidence as to how they may be generated, either by the insect or by the host. We studied one species without such lesions, the nine‐banded armadillo Dasypus novemcinctus, and two species with, the greater hairy armadillo Chaetophractus villosus and the southern three‐banded armadillo Tolypeutes matacus, both showing the characteristic ‘flea bite’ holes in the external surfaces of the osteoderms. Samples were studied by three‐dimensional backscattered electron mode scanning electron microscopy and X‐ray microtomography. Both methods showed resorption pit complexes in the external surfaces of the osteoderms characteristic of those made by osteoclasts in active bone resorption. Lesions involved both the syndesmoses (sutures) between adjacent bones and the central regions of the osteoderms. Many lesions showed extensive repair by infilling with new bone. We conclude that the T. perforans neosome creates a local host response which causes bone resorption, creating the space in which it can grow.
Beauty from within: Improvement of skin health and appearance with Lycomato a tomato‐derived oral supplement
Authors: Tarshish Elizabeth , Hermoni Karin 
Published: March 2023

AbstractBackroundHealthy and a youthful appearance is a common desire of the aging population. “Beauty from within” involves using nutrition and nutraceuticals to support skin function for reducing and reversing signs of aging such as wrinkles, pigmentary changes, skin laxity, and dullness. Carotenoids possess strong antioxidant and anti‐inflammatory activities and are effective in improving skin barrier and could thereby stimulate “beauty from within” by providing endogenous support to reduce the expressions of aging.AimThis study was designed to determine whether 3‐month supplementation with Lycomato would improve skin condition.MethodA panel of 50 female subjects used Lycomato capsules as nutritional supplements for 3 months. Skin status was observed via questionnaires for the assessment of skin condition and expert visual grading of facial markers such as wrinkles, tonality, roughness, laxity, and pore size. Skin barrier was assessed using transepidermal water loss (TEWL). Measurements were obtained before treatment and after 4 and 12 weeks of use.ResultsResults indicated a statistically significant improvement (p < 0.05) in skin barrier as measured by TEWL after 12 weeks of consuming the supplement. There was also a significant improvement in skin tonality, lines and wrinkles, pore size, and skin firmness as observed by expert evaluation as well as subject self‐assessment.ConclusionBased on the confines and conditions of this study, oral supplementation with Lycomato resulted in significant improvement in skin barrier. Visual appearance of lines and wrinkles, skin tonality, pores, smoothness, and firmness were considerably improved, and these improvements were found to be substantially discernible by the subjects.
Overweight in childhood and young adulthood increases the risk for adult thromboembolic events
Authors: Lilja Lina , Bygdell Maria , Martikainen Jari , Rosengren Annika , Kindblom Jenny M. , Ohlsson Claes 
Published: March 2023

AbstractBackgroundApproximately one third of thromboembolic (TE) events are related to obesity, but to which extent elevated body mass index (BMI) during the distinct periods of childhood and puberty contributes is not known. We aimed to evaluate the impact of high BMI during childhood and puberty for the risk of adult venous and arterial thromboembolic events (VTE, ATE, respectively) in men.MethodsWe included 37,672 men from the BMI Epidemiology Study (BEST) Gothenburg with data on weight and height in childhood, young adult age, and on pubertal BMI change. Information on outcomes (VTE [n = 1683], ATE [n = 144], or any first TE event [VTE or ATE; n = 1780]) was retrieved from Swedish national registers. Hazard ratios (HR) and 95% confidence intervals (CI) were estimated by Cox regressions.ResultsBoth BMI at 8 years of age and the pubertal BMI change were associated with VTE, independently of each other (BMI at 8: HR 1.06 per standard deviation [SD] increase, 95% CI, 1.01;1.11; pubertal BMI change: HR 1.11 per SD increase, 95% CI, 1.06;1.16). Individuals with normal weight during childhood followed by young adult overweight (HR 1.40, 95% CI, 1.15;1.72), and individuals with overweight at both childhood and young adult age (HR 1.48, 95% CI, 1.14;1.92), had a significantly increased risk of VTE in adult life, compared with the normal weight reference group. Individuals with overweight in childhood and in young adult age had increased risk of ATE and TE.ConclusionYoung adult overweight was a strong determinant, and childhood overweight a moderate determinant, of the risk of VTE in adult men.