bluuebunny/crossref_metadata_2025_split huggingface dataset:
Non-native PGPB Consortium Altered the Rhizobacterial Community and Slightly Stimulated the Growth of Winter Oilseed Rape (Brassica napus L.) Under Field Conditions
Authors: Dobrzyński J. , Kulkova I. , Jakubowska Z. , Wróbel B. 
Published: January 2025

AbstractPlant growth-promoting bacteria (PGPB) are among the most promising alternatives to mineral fertilizers. However, little is known about the effects of applied bacteria on the native microbiota, including the rhizobacterial community, which plays a crucial role in bacteria-plant interactions. Therefore, this study is aimed at assessing the effects of PGPB not only on plants but also, importantly, on the native rhizobacterial community of winter oilseed rape. The bacterial consortium, consisting of Pseudomonas sp. KR227 and Azotobacter PBC1 (P2A), slightly promoted plant growth, increasing the root weight by 21.95% and seed yield by 18.94%. This likely results from its ability to produce indole-3-acetic acid (IAA), solubilize phosphorus, and fix nitrogen, as indicated by a 35.76% increase in N-NH4 and a 35.05% increase in available phosphorus (AP). The introduced PGPB altered the rhizobacterial community of rapeseed, increasing the relative abundance of the phylum Proteobacteria and the genus Pseudomonas while decreasing the relative abundance of phylum Verrucomicrobiota (3 weeks after inoculation). Moreover, Proteobacteria were positively correlated with AP, while Verrucomicrobiota were correlated with N-NH4. At the genus level, Flavobacterium and Pseudomonas were positively correlated with AP, whereas Candidatus Udaeobacter showed a positive correlation with N-NH4 and a negative correlation with pH. Importantly, the P2A consortium did not significantly affect the diversity of native rapeseed rhizobacteria. These findings suggest that the tested P2A consortium has potential as a biostimulant in rapeseed cultivation.
Establishing reference curves for vital tissue perfusion using quantitative near-infrared fluorescence imaging with indocyanine green
Authors: Tange Floris P. , Peul Roderick C. , van den Hoven Pim , Koning Stefan , Kruiswijk Mo W. , Faber Robin A. , Verduijn Pieter S. , van Rijswijk Carla S. P. , Galema Hidde A. , Hilling Denise E. , van Dijk Sam P. J. , van Ginhoven Tessa M. , Keereweer Stijn , Mureau Marc A. M. , Feitsma Eline A. , Noltes Milou E. , Kruijff Schelto , Driessen Caroline , Achiam Michael P. , Schepers Abbey , van Schaik Jan , Mieog J. Sven D. , Vahrmeijer Alexander L. , Hamming Jaap F. , van der Vorst Joost R. 
Published: January 2025

Abstract
Purpose
Assessment of tissue perfusion using near-infrared fluorescence (NIR) with indocyanine green (ICG) is gaining popularity, however reliable and objective interpretation remains a challenge. Therefore, this study aimed to establish reference curves for vital tissue perfusion across target tissues using this imaging modality.

Methods
Data from five prospective study cohorts conducted in three Dutch academic medical centres between December 2018 and June 2023 was included. Quantitative analysis using time-intensity curves was performed in ten target tissues, including the colon, ileum, gastric conduit, deep inferior epigastric artery perforator (DIEP) flap, skin of the foot, trachea, sternocleidomastoid muscle (SCM), carotid artery, parathyroid gland, and skin of the neck.

Results
A total of 178 patients were included in this study, representing 303 target tissues. Three different patterns of reference curves were identified based on a subjective assessment. Seven out of ten tissues showed a reference curve with rapid inflow (median time-to-max (tmax): 13.0–17.8 s, median maximum-normalized-slope (slope norm): 10.6–12.6%/sec), short outflow (median area-under-the-curve of tmax + 60 s (AUC60): 65.0–85.1%) followed by a gradual/absent outflow. Secondly, the DIEP flap and SCM tissue showed a reference curve with longer inflow (median tmax: 24.0, 22.0 s, median slope norm: 9.3, 9.7%/sec respectively) and reduced outflow (median AUC60: 89.1, 89.0% respectively). Thirdly, the skin of the foot showed slow inflow (median tmax 141.1 s, median norm slope 2.1%/sec) without outflow.

Conclusion
This study demonstrates reference curves for vital tissue perfusion of multiple target tissues identified with ICG NIR fluorescence imaging, providing a critical step towards the clinical implementation of this technique.
Fracture load of feldspar ceramic crowns: effects of surface treatments and aging
Authors: Coldea Andrea , Stawarczyk Bogna , Meinen John , Lankes Valerie , Swain Michael V. , Roos Malgorzata 
Published: January 2025

Abstract

Objectives
To compare the impact of intaglio surface treatments – airborne particle abrasion and hydrofluoric acid (HF) etching – of feldspar ceramic (FEL) crowns on the fracture load (FL) and to investigate the effects of abutment materials and artificial aging. The aim was to assess whether etching could be replaced by an alternative surface roughening method.

Materials and methods
FEL crowns had their intaglio surfaces either abraded (25 µm Al2O3, 0.1 MPa), etched (HF, 60 s), or untreated and then bonded to CoCrMo- and polymer-abutments. FL was measured for non-aged and aged (1.2 million mastication cycles) specimens. Data were analyzed using, Weibull modulus, two-/one-way ANOVA with Tukey HSD-post-hoc-test, t-tests, and TOST equivalence (p < 0.05).

Results
For crowns bonded to CoCrMo abutments, aging affected the FL and Weibull modulus, but pretreatment methods did not. For initial specimens, airborne abraded and etched crowns were equivalent within a 400N bound, however, for aged specimens, equivalence was inconclusive. For crowns bonded to polymer-abutments, pretreatment and aging influenced the FL. Etching decreased the initial FL by over 420N compared to airborne abraded and untreated specimens. After aging, untreated crowns’ FL decreased by 528N, while airborne abraded and etched specimens showed no aging effect.

Conclusions
Airborne particle abrasion of FEL crowns’ intaglio surfaces did not negatively impact FL and was comparable to etched crowns. Conclusions regarding pretreatment methods and aging differed between CoCrMo- and polymer-abutments.
Clinical relevance.
Airborne particle abrasion may be an alternative procedure for the intaglio surface treatment of FEL crowns prior bonding.
Exact dimension reduction for rough differential equations
Authors: Redmann Martin , Riedel Sebastian 
Published: January 2025

AbstractIn this paper, practically computable low-order approximations of potentially high-dimensional differential equations driven by geometric rough paths are proposed and investigated. In particular, equations are studied that cover the linear setting, but we allow for a certain type of dissipative nonlinearity in the drift as well. In a first step, a linear subspace is found that contains the solution space of the underlying rough differential equation (RDE). This subspace is associated to covariances of linear Ito-stochastic differential equations which is shown exploiting a Gronwall lemma for matrix differential equations. Orthogonal projections onto the identified subspace lead to a first exact reduced order system. Secondly, a linear map of the RDE solution (quantity of interest) is analyzed in terms of redundant information meaning that state variables are found that do not contribute to the quantity of interest. Once more, a link to Ito-stochastic differential equations is used. Removing such unnecessary information from the RDE provides a further dimension reduction without causing an error. The resulting reduced order rough equation can be solved numerically much faster than the original system. Therefore, our approach provides enormous savings in computing time and is hence beneficial from the practical point of view. Finally, we discretize a linear parabolic rough partial differential equation and a rough wave equation in space. The resulting large-order RDEs are subsequently tackled with the exact reduction techniques studied in this paper. We illustrate the enormous complexity reduction potential in the corresponding numerical experiments.
Linkage Between Vertical Coupling and Storage Flux: Insights from Urban Tall-Tower Eddy Covariance Measurement
Authors: Lan Changxing , Holst Christopher Claus , Grünwald Thomas , Mauder Matthias 
Published: January 2025

Abstract
The storage flux, corresponding to disequilibrium between observed flux and net surface emissions, poses a significant source of uncertainty in tower-based eddy covariance (EC) measurements over urban and forest ecosystems. In this study, we investigated the coupling between the urban inertial sub-layer (ISL) and roughness sub-layer (RSL) and its influence on nighttime storage flux, leveraging tower-EC together with collocated wind profile measurements. Our findings demonstrate that substantial storage flux occurs when the gradient of turbulent kinetic energy (TKE) enlarges, indicating decoupling between ISL and RSL. With increasing wind speed, turbulent eddies generated by bulk wind shear directly interact with the surface, conducive to the recoupling between ISL and RSL and resulting in decreased storage flux. Conversely, when the gradient of TKE between ISL and RSL is small, the storage flux remains low and relatively insensitive to wind speed. The derived diagnostic relation further confirms the predominant influence of stability and turbulent intensity gradient on regulating the storage flux. These results provide valuable insights as a complement to prior storage flux studies in the context of canopy flow.
The impact of surgical technique on the number of sentinel lymph nodes removed and its effect on complication rates
Authors: Stutz Kathleen , Mason Holly , Niakan Shiva , Coulter Aixa Perez , Casaubon Jesse , Friedrich Ann-Kristin U. 
Published: January 2025

Abstract
Purpose
Sentinel lymph node biopsy (SLNB) is a staging procedure used to guide treatment for patients with breast cancer. Multiple variations in the SLNB technique have been described. We questioned how technique impacts the number of sentinel lymph nodes (SLNs) removed and associated complications.

Methods
Patients with breast cancer who were treated with lumpectomy and SLNB between 2018 and 2023 were analyzed. Patients were excluded if they had prior ipsilateral breast or axillary surgery or chest wall radiation, underwent neoadjuvant chemotherapy or endocrine therapy, or subsequently required ALND. Demographics, surgical technique, and operative and pathological data were collected. Complication rates were compared between more (4+) or fewer (1–3) SLNs removed.

Results
A total of 643 patients were included, with an average of 2.44 LNs removed (range 1–11). The overall complication rate was 19.8%, with a 4.4% lymphedema rate. The lymphedema rate was higher among patients who had more nodes removed. An average of 2.5 LNs were removed with dual mapping vs. 2.0 with technetium alone (p = 0.15). Breast massage had no effect on the number of SLNs removed (p = 0.12) but did impact blue dye uptake (p = 0.001).

Conclusions
Surgical technique did not significantly impact the number of nodes removed. Removing more nodes was associated with a greater risk of lymphedema.
Individual movement behaviour and habitat use of a small-sized cypriniform (Telestes muticellus) in a mountain stream
Authors: Schiavon Alfredo , Comoglio Claudio , Candiotto Alessandro , Spairani Michele , Hölker Franz , Watz Johan , Nyqvist Daniel 
Published: January 2025

Abstract
Knowledge about the biology and ecology of species is fundamental for their management and conservation. Despite this, many fish species and life stages are understudied, and there is a great need for research efforts to understand their ecology. Italian riffle dace (Telestes muticellus; order Cypriniformes) is a small-sized (< 15 cm) stream fish native to the Italian peninsula. There is a scarcity of research on its ecology and behaviour. In this study, we explored the movement ecology of Telestes muticellus in a Mediterranean mountain stream in Northern Italy using passive integrated transponder (PIT) telemetry. Over 15 months, 267 fish were located 2–27 times. Most fish remained stationary, while a few roamed over several hundreds of meters. Fish covered a larger linear range during spring and autumn than during other seasons. T. muticellus showed a strong preference for pools, and this preference was strongest during summer. We observed no differences in habitat use and movements between day and night, and fish size had no major effect. Within pools, many T. muticellus displayed remarkably small home ranges (median 8 m2). Fish increased their linear ranges over a period of flood events, indicating that high-flow events may be important for the downstream and upstream dispersal of T. muticellus in small mountain streams.
Genomic determinants of biological age estimated by deep learning applied to retinal images
Authors: Huang Yu , Syed Mohammad Ghouse , Chen Ruiye , Li Cong , Shang Xianwen , Wang Wei , Zhang Xueli , Zhang Xiayin , Tang Shulin , Liu Jing , Liu Shunming , Srinivasan Sundar , Hu Yijun , Mookiah Muthu Rama Krishnan , Wang Huan , Trucco Emanuele , Yu Honghua , Palmer Colin , Zhu Zhuoting , Doney Alexander S. F. , He Mingguang 
Published: January 2025

Abstract
With the development of deep learning (DL) techniques, there has been a successful application of this approach to determine biological age from latent information contained in retinal images. Retinal age gap (RAG) defined as the difference between chronological age and predicted retinal age has been established previously to predict the age-related disease. In this study, we performed discovery genome-wide association analysis (GWAS) on the RAG using the 31,271 UK Biobank participants and replicated our findings in 8034 GoDARTS participants. The genetic correlation between RAGs predicted from the two cohorts was 0.67 (P = 0.021). After meta-analysis, we found 13 RAG loci which might be related to retinal vessel density and other aging processes. The SNP-wide heritability (h
2) of RAG was 0.15. Meanwhile, by performing Mendelian randomization analysis, we found that glycated hemoglobin, inflammation hemocytes, and anemia might be associated with accelerated retinal aging. Our study explored the biological implications and molecular-level mechanism of RAG, which might enable causal inference of the aging process as well as provide potential pharmaceutical intervention targets for further treatment.

Graphical Abstract
Prognostic impact of glucagon-like peptide-1 receptor (GLP1R) expression on cancer survival and its implications for GLP-1R agonist therapy: an integrative analysis across multiple tumor types
Authors: Ungvari Zoltan , Bartha Áron , Ungvari Anna , Fekete Monika , Bianchini Giampaolo , Győrffy Balázs 
Published: January 2025

AbstractGlucagon-like peptide-1 receptor (GLP-1R) agonists, such as exenatide (Byetta, Bydureon), liraglutide (Victoza, Saxenda), albiglutide (Tanzeum), dulaglutide (Trulicity), lixisenatide (Lyxumia, Adlyxin), semaglutide (Ozempic, Rybelsus, Wegovy), and tirzepatide (Mounjaro, Zepbound), are widely used for the treatment of type 2 diabetes mellitus (T2DM) and obesity. While these agents are well known for their metabolic benefits, there is growing interest in their potential effects on cancer biology. However, the role of GLP-1R agonists in cancer remains complex and not fully understood, particularly across different tumor types. This study aimed to evaluate the prognostic significance of GLP1R expression on overall survival across various cancer types. Using a comprehensive analysis of gene expression data and survival outcomes a large cohorts of different tumor types, we employed Cox proportional hazards survival analyses, coupled with false discovery rate determinations, to explore correlations between GLP1R expression and survival. The integrated database included thousands of cancer specimens with available overall survival time and event data from numerous independent cohorts, providing a robust platform for survival analysis. Our findings reveal that increased GLP1R expression is associated with improved overall survival in cancers such as bladder cancer, breast cancer, esophageal adenocarcinoma, renal clear cell carcinoma, and thyroid carcinoma. Conversely, higher GLP1R expression is linked to poorer survival outcomes in cervical squamous cell carcinoma, lung squamous cell carcinoma, stomach adenocarcinoma, and uterine corpus endometrial carcinoma. Additionally, GLP1R expression showed no significant impact on overall survival in cancers such as esophageal squamous cell carcinoma, colon cancer, head-neck squamous cell carcinoma, renal papillary cell carcinoma, hepatocellular carcinoma, lung adenocarcinoma, ovarian cancer, and pancreatic cancer. In conclusion, GLP1R expression levels serve as an important biomarker with potential prognostic significance across multiple cancers, demonstrating both protective and adverse associations depending on the tumor type. These findings highlight the complex role of GLP-1R agonists in cancer risk and survival, suggesting that the therapeutic use of these agents should be carefully tailored to the individual patient’s cancer risk profile.
Artificial Intelligence in Metal–Organic Frameworks from 2013 to 2024: A Bibliometric Analysis
Authors: Cao Jian , Zhou Ling , Gan Fan , You Zhipeng 
Published: January 2025

Abstract
The purpose of this work is to analyze the development direction and prospects in the field of artificial intelligence (AI) in metal–organic frameworks (MOFs) and to provide reference information for related research and industry personnel. The scientific papers on AI in MOFs published in Web of Science database from 2013 to mid-2024 were collected. Bibliometric methods and knowledge mapping visualization software were used to analyze the papers. Both quantitative statistics and qualitative comparative analysis of global scientific papers were done in terms of annual paper trends, papers by major countries, authors, institutions, journals and research topics, respectively. The results showed that the number of published papers has increased in recent years. The top three productive countries are China, the USA and Germany, respectively. The top three productive institutions are Guangzhou University, Northwestern University and Chinese Academy of Sciences, respectively. Reference co-citation analysis classifies references into four clusters, and keyword co-occurrence analysis divides keywords into six clusters. Bibliometric and network analyses were utilized to examine the distribution of research outcomes, enabling scholars to discern the prevailing trends and focal points within the domain of AI-MOFs.
Endocrine Disruptors in Child Obesity and Related Disorders: Early Critical Windows of Exposure
Authors: Celik Mensure Nur , Yesildemir Ozge 
Published: January 2025

Abstract
Purpose of Review
Endocrine disruptors (EDs) can mimic or interfere with hormones in the body, leading to non-communicable diseases, such as obesity, diabetes, and metabolic syndrome. Susceptibility to EDs increases during prenatal and postnatal life, a critical time window. This review aims to summarize the latest evidence on the relation of early life exposure to some EDs with obesity and the other metabolic disorders.

Recent Findings
There is increasing evidence that early life exposure to EDs may impair adipogenesis by increasing the number and size of adipocytes, thereby increasing susceptibility to obesity in childhood. It is stated that exposure to EDs during the prenatal and postnatal period may raise the risk of type 2 diabetes in adulthood by disrupting glucose, lipid, and insulin homeostasis in the offspring. They can also accelerate the development of type 1 diabetes through various mechanisms, like immunomodulation, gut microbiota, and vitamin D pathways.

Summary
There is a growing understanding that ED exposure during critical stages of life could play an important role in the development of obesity and metabolic disorders. We suggest setting national goals, global standards, and policies to reduce environmental exposure to pregnant and lactating women, and babies, considered sensitive populations.
Boosting electrocatalytic generation of FDCA and H2 from 2,5-furanedimethanol solution by carbonized wood supported Fe-CoP nanoleaves
Authors: Ma Yuan , Miao Jiaojiao , Li Yuanyuan , Zhou Xuehua , Zhang Lianbing , Zhang Jian , Li Guanglei , Qin Yong , Gao Jie 
Published: January 2025

AbstractElectrocatalytic oxidation of more stable 2,5-furanedimethanol (FDM) for 2,5-furanediformic acid (FDCA) generation with concurrent hydrogen production is attractive but still nascent compared to 5-Hydroxymethyl-2-furaldehyde (HMF). The need for effective and stable bifunctional electrocatalysts that are efficient for the FDM cell is thus quite significant. Wood serves as an ideal matrix for boosting the performance of catalysts, since its hierarchical porous structures facilitate mass transport and provide abundant active sites. Unfortunately, it has never been demonstrated for electrochemically organic synthesis. Herein, the effectiveness of Fe-CoP in catalyzing FDM oxidation was demonstrated by density functional theory (DFT) calculations and experiments, and a renewable carbonized porous wood decorated with Fe-doped CoP nanoleaves (Fe-CoP/CW) was constructed for electrocatalytic FDCA and hydrogen generation. The obtained Fe-CoP/CW as an anode in FDM solution afforded a current density of 100 mA cm−2 with a yield of 90% FDCA at a potential no more than 1.50 V vs RHE, which was 90 mV and 350 mV lower than Fe-CoP/carbon cloth (CC) and IrO2. In addition, Fe-CoP/CW showed excellent long-term stability for 108-h FDM oxidation in strong alkaline solution. Remarkably, in stark contrast to Fe-CoP/CC and Pt, the hydrogen evolution performance of Fe-CoP/CW was not impacted by FDM at the cathode, and it required exceptionally low overpotentials of 0.19 V to achieve 100 mA cm−2. As a result, in terms of the overall cell, the hydrogen production rate was 0.756 mmol cm−2 h−1, which was 3.57 times higher than those of commonly used commercial Pt | IrO2 cell, presenting a Faraday efficiency of near 100%. This work will pave the way towards the implementation of highly suited bifunctional electrodes and the possibility of affordable, effective, and environmentally-friendly wood-derived electrocatalysts for electrochemically organic synthesis.
Graphical Abstract