bluuebunny/crossref_metadata_2025_split huggingface dataset:
Polymorphic Single‐Nucleotide Variants in miRNA Genes and the Susceptibility to Colorectal Cancer: Combined Evaluation by Pairwise and Network Meta‐Analysis, Thakkinstian's Algorithm and FPRP Criterium
Authors: Liu Qing , Archilla Ivan , Lopez‐Prades Sandra , Torres Ferran , Camps Jordi , Cuatrecasas Miriam 
Published: January 2025

ABSTRACTBackgroundConsiderable epidemiological studies have examined the correlation between polymorphic single‐nucleotide variants (SNPs) in miRNA genes and colorectal carcinoma (CRC) risk, yielding inconsistent results. Herein, we sought to systematically investigate the association between miRNA‐SNPs and CRC susceptibility by combined evaluation using pairwise and network meta‐analysis, the FPRP analysis (false positive report probability), and the Thakkinstian's algorithm.MethodsThe MEDLINE, EMBASE, WOS, and Cochrane Library databases were searched through May 2024 to find relevant association literatures. Pooled odds ratios (ORs) and 95% confidence intervals (CIs) were computed by the pairwise meta‐analysis. Network meta‐analysis and the Thakkinstian's method were applied for determining the potentially optimal genetic models; additionally, the FPRP was used to identify noteworthy associations.ResultsTotally, 39 case–control trials involving 18,028 CRC cases, and 21,816 normal participants were included in the study. Eleven SNPs within nine genes were examined for their predisposition to CRC. miR‐27a (rs895819) was found to significantly increase CRC risk among overall population (OR 1.58, 95% CI: 1.32–1.89) and Asians (OR 1.62, 95% CI: 1.31–2.01), with the recessive models identified as the optimal models. Furthermore, miR‐196a2 (rs11614913), miR‐143/145 (rs41291957), and miR‐34b/c (rs4938723) were significantly related to reduced CRC risk among Asian descendants under the optimal dominant (OR 0.75, 95% CI: 0.65–0.86), recessive (OR 0.72, 95% CI: 0.60–0.85), and recessive models (OR 0.69, 95% CI: 0.56–0.85), respectively. The results were also proposed by the network meta‐analysis or the Thakkinstian's method and confirmed by the FPRP criterion.ConclusionThe miR‐27a (rs895819) is correlated with elevated CRC risk among overall population and Asians, and the recessive model is found to be optimal for predicting CRC risk. Additionally, the miR‐196a2 (rs11614913), miR‐143/145 (rs41291957), and miR‐34b/c (rs4938723), with the dominant, recessive, and recessive models identified as the optimal, might confer protective effects against CRC among Asians.
Impact of Soil Factors and Climate on Phytochemical Characteristics of Stachys lavandulifolia Vahl.
Authors: Zamani Zahra , Tamartash Reza , Heydari Qodratollah , Jelodar Zeinab Jafarian 
Published: January 2025

ABSTRACTThis study investigates the influence of environmental factors on the secondary metabolites of Stachys lavandulifolia Vahl., focusing on how soil properties, temperature, and precipitation affect the yield and chemical composition of its essential oils. The research was conducted in two domains within three rangelands in Mazandaran province, Iran. Data were analyzed using SPSS software. The findings indicated significant variations in essential oil yield across the southern domains of all three study areas. Variance analysis revealed that the interaction between area and domain significantly influenced most of the essential oil compositions, with exceptions for α‐copaene, α‐thujene, spathulenol, germacrene‐D, hexadecanoic acid, and cis‐farnesol. The mean comparison demonstrated substantial differences in the compositions of α‐pinene, α‐thujene, myrcene, phytol, and bicyclogermacrene between the northern and southern domains. Furthermore, correlation analysis identified significant relationships between essential oil compositions and various soil factors, such as moisture content, soil stability index, bulk density, phosphorus (P), potassium (K), calcium (Ca), calcium oxide (CaO), magnesium (Mg), sodium (Na), nitrogen (N), sodium adsorption ratio, silt, clay, and organic matter. However, due to the favorable habitat conditions, no significant correlations were observed between temperature, rainfall, and the essential oil compositions in these areas.
Charge Modification of Lysine Mitigates Amyloid‐β Aggregation
Authors: Kwon HaeMin , Kim JiMin , Park InWook , Ye Suhyun , Park MinSeol , Yang Seung‐Hoon , Kim YoungSoo , Yun Kim Hye 
Published: January 2025

AbstractAlzheimer's disease (AD) is a progressive neurodegenerative condition characterized by the deposition of amyloid‐β (Aβ) peptides, which aggregate into toxic structures such as oligomers, fibrils, and plaques. The presence of these Aβ aggregates in the brain plays a crucial role in the pathophysiology, leading to synaptic dysfunction and cognitive impairment. Understanding how physiological factors affect Aβ aggregation is essential, and therefore, exploring their influence in vitro will likely provide insights into their role in AD pathology. In this study, we investigated the effects of physiological, free amino acids on Aβ aggregation dynamics. We focused on positively charged amino acids, particularly lysine, and employed a chemical modification, methylation, to neutralize its charge. Our analyses revealed that modified lysine significantly reduced Aβ aggregation, indicating that charge distribution of amino acids plays a crucial role in modulating Aβ aggregation behavior. These findings enhance our understanding of the regulatory factors influencing Aβ aggregation and highlight important considerations for future research on Aβ.
Probing DNA G‐Quadruplex and I‐Motif Structures Via a Fluorescent Nucleoside Analogue: 4‐Cyanoindole‐2′‐Deoxyribonucleoside
Authors: Li Danqi , Zhuang Bo , Feng Ran‐ran , Gai Feng 
Published: January 2025

AbstractSince the building blocks of DNA are nonfluorescent, various external fluorescence reporters have been employed to investigate the structure, dynamics, and function of DNA G‐quadruplexes (GQs) and i‐motifs (iMs), which play an important role in gene regulation and expression. However, most of those fluorescence reporters lack the ability to provide site‐specific structural information of interest. Therefore, it is necessary to develop fluorescent nucleoside analogues that can be covalently inserted into oligonucleotides, which not only serve this purpose, but minimize any potential perturbation towards the native structure of the DNA systems in question. Herein, we characterize the spectroscopic utility of a high quantum yield fluorescent nucleoside analogue, 4‐cyanoindole‐2′‐deoxyribonucleoside (4CNI‐NS). We show that (1) incorporation of 4CNI‐NS into various oligonucleotides does not alter their ability to fold into their respective native structures, nor does it affect the overall stability of those structures and (2) the fluorescence property of 4CNI‐NS is sensitive to its local environment, and the fluorescence intensity and decay kinetics of the 4CNI‐NS‐containing oligonucleotides exhibit a clear dependence on their secondary structure formation. Collectively, our results demonstrate that 4CNI‐NS can be used as a sensitive, isomorphic probe in the study of noncanonical DNA structures.
Electrochemical Deconstruction of Waste Polyvinylidene Chloride (PVDC) to Value‐Added Products in Batch and Flow
Authors: Mishra Subhadip , Kar Sourav , Rangappa Raghavendrakumar , Patil Prashant , Kadam Vijay , Chikkali Samir H. , Samanta Ramesh C. 
Published: January 2025

AbstractChlorinated polymers have made enormous contributions to materials science and are commercially produced on a large scale. These chlorinated polymers could be recycled as chlorine sources to efficiently produce valuable chlorinated compounds owing to their facile release of HCl. Although the thermal stability of PVDC is low compared to PVC, this can be advantageous in terms of easy and fast dehydrochlorination. Herein, we report an efficient electrochemical chlorination using poly(vinylidene chloride) (PVDC) as a chlorine source that works in an undivided cell and applies to a good number of examples. This method works on commodity polymers such as waste PVDC‐PVC pharma blister film, PVDC‐PO multilayer food packaging, and compression molded sheets of Ixan PVDC (with heat stabilizer) with similar efficiency. Furthermore, this method also provides the dechlorination of PVDC up to 98 %, leading to unsaturated dechlorinated material. Converting PVDC into more stable unsaturated compounds, the release of harmful chlorine‐containing gases during incineration can be minimized. Additionally, this method is not only restricted to batch processes but an electroflow process for PVDC dechlorination and electrosynthesis has also been demonstrated.
Synthesis and Chiroptical Activity of π‐Expanded Electron‐Rich Heterohelicenes Based on the 1,4‐Dihydropyrrolo[3,2‐b]pyrrole Core
Authors: Kusy Damian , Górski Krzysztof , Bertocchi Francesco , Galli Matteo , Vanthuyne Nicolas , Terenziani Francesca , Gryko Daniel T. 
Published: January 2025

AbstractHerein, we report the synthesis and chiroptical characteristics of the first (double) helicenes possessing the 1,4‐dihydropyrrolo[3,2‐b]pyrrole (DHPP) moiety as their central core. We have developed a three‐step synthesis with 6π‐electrocyclization accompanied by HBr elimination as its key step. We found that, whereas for smaller peripheral arms double 6π‐electrocyclization occurs smoothly forming a double helicene, in the case of longer polycyclic aromatic hydrocarbons the reaction becomes less efficient and mono‐helicenes are the only products. The electron density distribution analysis of LUMO explains the different regioselectivity of 6π‐electrocyclization. The synthesized heterohelicenes are characterized by greenish‐blue emission, distinct solvatofluorochromism and good fluorescence quantum yields (up to 42 %). Moreover, the chiroptical measurements reveal that unsymmetrical double heterohelicene exhibits excellent circularly polarized luminescence brightness (BCPL) reaching 30 M−1 cm−1. The combined experimental and computational study has revealed that a charge‐transfer state is responsible for the observed emission (hence the solvatochromism), while low‐energy absorption derives from multiple transitions.
A Unique Intramolecular Redox Reaction by Unidirectional Migration of Three Hydrogen Atoms: Theoretical Elucidation of the 3H‐Transfer Sequence
Authors: Arndt Thiemo , Schäfer Mathias , Kuck Dietmar , Breugst Martin 
Published: January 2025

AbstractThe intramolecular migration of three hydrogen atoms from one moiety of a gaseous radical cation to the other prior to fragmentation is an extremely rare type of redox reaction. Within the scope of this investigation, this scenario requires an ionized but electron‐rich arene acceptor bearing a para‐(3‐hydroxyalkyl) residue. The precise mechanism of such unidirectional 3H transfer processes, including the order of the individual H transfer steps, has remained unclear in spite of previous isotope labelling and recent infrared ion spectroscopy (IRIS) studies. Herein, the details of this peculiar process have been investigated for ionized 4‐(N,N‐dimethylaminophenyl)‐2‐butanol, 2, by state‐of‐the‐art density functional theory (DFT) calculations. The energetically most favorable pathway consists of a sequence of successive 1,4‐, 1,6‐ and 1,5‐H steps. During these steps the secondary alcohol functionality is oxidized to a carbonyl group and the radical‐cationic aniline ring is reduced to an ionized 2,3–dihydroaniline unit. Several alternative sequences, such as three successive 1,5‐H shifts, could be excluded. A concomitant unidirectional 2H migration reaction of ion 2 was also investigated and the intermediacy of ion‐neutral complexes (INCs) enabling sequential hydride and proton transfer was confirmed by the calculations.
Operando Luminescence Thermometry of a Solid Catalyst in a Reactor during a High‐Temperature Chemical Process
Authors: Groefsema Daniël W. , Rabouw Freddy T. , Boele Michiel , van Bavel Alexander P. , Weckhuysen Bert M. 
Published: January 2025

AbstractIn most catalytic processes, thermal energy is released or consumed locally in a reactor due to the exothermicity or endothermicity of the chemical reactions. This causes the actual operating temperature of the catalyst material to deviate from the reactor temperature, which can lead to underperformance, reduced catalyst stability, or even thermal runaway. Conventional methods to measure the catalyst temperature, with a thermocouple in the reactor oven or in the catalyst bed, suffer from complications because of separation between the sensor and catalyst material, chemical activity of the thermocouple/coating material, added complexity of the setup, and disruption of the reactant flow and heat flow through the catalyst bed. Here, we show the possibilities and challenges of luminescence thermometry as an analytical technique for remote temperature monitoring of the local catalyst temperature in a strongly exothermic high‐temperature reaction environment. We observe that the luminescence lifetime depends not only temperature, but also on oxygen concentration, which can introduce a significant systematic error of up to 40 °C in the recorded temperature. In the case of ratiometric luminescence thermometry, this error is strongly reduced to ~5 °C. We use the ratiometric technique to confirm its applicability in the exothermic oxidative coupling of methane (OCM) process at high reaction temperatures, showing an exothermic increase in the local catalyst temperature of up to 100 °C, relative to the constant reactor temperature under inert conditions.
Aberrant Expression of JAM2 Inhibits Invasion and Migration in Lung Adenocarcinoma
Authors: Chen Jun , Cui Yuan , Chen Zhimeng , Ding Hao , Li Chang , Ju Sheng , Ding Cheng , Xu Chun , Zhao Jun , Tong Xin 
Published: January 2025

ABSTRACTBackgroundLung adenocarcinoma (LUAD) is the most common histological subtype of lung cancer. JAM2, a member of the Junctional adhesion molecule (JAM) family, plays diverse roles in cell–cell contacts and tumor development. Although JAM2's expression and functions have been reported in various cancers, its clinical and biological significance in LUAD remains unclear.AimsThe aim of this study was to investigate the expression and function of JAM2 in LUAD, and to assess its potential as a prognostic gene and a molecular target for early diagnosis and targeted therapy.MaterialsImmunohistochemistry (IHC) was performed on 37 pairs of LUAD tissues. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses were conducted among co‐expression genes in different JAM2 subgroups. In vitro experiments were also conducted to study the migratory and invasive capabilities of LUAD cells when JAM2 was overexpressed.ResultsThe study confirmed that JAM2 was downregulated in LUAD, possibly due to methylation. JAM2 emerged as an independent prognostic gene for predicting the outcomes of patients with LUAD. IHC analysis revealed the significance of JAM2 with clinicopathological parameters in LUAD. GO and KEGG analyses provided insights into the biological processes and pathways associated with JAM2. In vitro experiments showed that overexpressing JAM2 significantly suppressed the migratory and invasive capabilities of LUAD cells. Additionally, JAM2 played a crucial role in LUAD inflammatory infiltration, and higher JAM2 expression predicted a better immunotherapy response.ConclusionJAM2 may serve as a promising molecular target for early diagnosis and targeted therapy of LUAD. Its downregulation in LUAD, potential role as a prognostic gene, and influence on cell migration, invasion, and inflammatory infiltration make it a valuable target for further research and development of therapeutic strategies.
High‐Performance Flexible Compact Wideband U‐Slot CPW‐Fed Textile Antenna for WBAN, WLAN, ISM, and WI‐FI 6E Applications
Authors: Bouaza Abdelmaoula , Touhami Rachida , Tedjini Smail , Jabbar Abdul , Ur‐Rehman Masood , Ramzan Naeem 
Published: January 2025

ABSTRACTThis paper presents a novel, wideband, and flexible coplanar waveguide (CPW) antenna design on a low‐cost denim substrate. The antenna exhibits conformal properties and achieves robust dual‐band performance. The antenna achieves a maximum gain of 6.18 dB at 6.5 GHz and a peak efficiency of 98% at 2.45 GHz in normal conditions. The fabricated prototype demonstrates excellent agreement between simulated and measured results. The simulated impedance bandwidth ranged from (2.20–3 GHz) for the first band and (4.41–7.43 GHz) for the second band. The measured results show that it offers −10 dB impedance bandwidths of 22% (2.2–2.75 GHz) for WBAN applications and 68% (3.63–7.43 GHz) for WLAN and WIFI‐6E applications through strategically designed slots in the ground plane (symmetric U‐slot and rectangular slot). Furthermore, the antenna's performance remains stable under various bending conditions and exhibits satisfactory behavior close to the human body, making it suitable for wearable applications. This cost‐effective design presents a promising solution for integration into various consumer electronics. The proposed antenna design is a low‐cost solution for various wearable consumer electronics applications. This antenna is a potential solution for enhanced workforce management, secure access control, and identification.
The use of endoscopic ultrasound in tandem with endoscopic retrograde cholangiopancreatography in the 2019 American Society for Gastrointestinal Endoscopy guideline for patients at high risk of choledocholithiasis can help to avoid diagnostic endoscopic retrograde cholangiopancreatography in individuals without ascending cholangitis
Authors: Sirinawasatien Apichet , Chanchairungcharoen Jiravit , Yaowmaneerat Thanapon , Jiratham‐opas Jirat , Chanpiwat Kanokpoj , Chantarojanasiri Tanyaporn , Attasaranya Siriboon , Laohavichitra Kannikar , Wannaprasert Jerasak , Ratanachu‐ek Thawee 
Published: January 2025

AbstractObjectivesCholedocholithiasis is the leading cause of biliary pancreatitis and biliary sepsis. Endoscopic retrograde cholangiopancreatography (ERCP) is considered a minimally invasive treatment for choledocholithiasis. However, diagnostic ERCP should be avoided. We conducted a prospective trial in high‐risk choledocholithiasis patients based on the American Society for Gastrointestinal Endoscopy (ASGE) 2019 criteria to investigate the diagnostic accuracy of the current guideline.MethodsThis multicenter, prospective cohort study included 240 consecutive patients. The primary outcome was the performance of the criteria in predicting choledocholithiasis. The secondary outcome was a percentage reduction in diagnostic ERCP when endoscopic ultrasound was used in tandem with ERCP in individuals without ascending cholangitis.ResultsThe overall criteria revealed a positive common bile duct (CBD) stone in 87.1% of patients. Regarding the diagnostic performance of each criterion, ascending cholangitis had a specificity of 67.7% and a positive predictive value (PPV) of 90.2%; total bilirubin >4 mg/dL and dilated CBD had a specificity of 74.2% and a PPV of 55.6%; and CBD stone on ultrasound/cross‐sectional imaging had a specificity of 58.1% and a PPV of 89.2%. Of the 138 patients without ascending cholangitis who met the other two high‐risk criteria and were sent for EUS first, 21 cases (15.2%) were able to avoid a diagnostic ERCP.ConclusionsThe current ASGE 2019 criteria yield acceptable choledocholithiasis diagnostic accuracy. Using endoscopic ultrasound to confirm CBD stones before ERCP can help almost half of patients with the specific condition of total bilirubin >4 mg/dL and dilated CBD to avoid diagnostic ERCP.
The influence of the forest corridors to the north of the Andes on the diversification of the bright‐rumped Attila, Attila spadiceus (Passeriformes, Tyrannidae), during the climatic oscillations of the middle Pleistocene
Authors: Mendonça Patrícia , Carneiro Lincoln Silva , Leandro‐Silva Victor , Aleixo Alexandre , Araripe Juliana , Rêgo Péricles S. 
Published: January 2025

AbstractThis study aims to enhance our understanding of the temporal and spatial processes scales governing the evolutionary diversification of Neotropical birds with Trans‐ and Cis‐Andean populations of the species Attila spadiceus from South and Central America. Through a multilocus analysis of the mitochondrial (CytB and ND2) and nuclear genes (I7BF, I5BF, and G3PDH) of 41 samples representing six subspecies, we describe the existing molecular lineages of A. spadiceus, and estimate their demographic dynamics. We used Ecological Niche Modeling (ENM) with six different algorithms to predict the potential distribution of A. spadiceus in both present‐day and past scenarios, examining the overlap climatic niche between Cis‐ and Trans‐Andean lineages. The analysis confirms a relatively recent divergence of the Trans‐ and Cis‐Andean lineages, at approximately 0.25 million years ago (Ma). The niche modeling supports the existence of a dynamic scenario of the expansion and retraction of forest corridors in northwestern South America during the last glaciation. This suggests that the earlier orogenesis of the Andes was not a primary determinant of this dichotomy. Additionally, the analysis of population dynamics indicated a trend of increasing population size starting at 0.05 Ma for both lineages. Our findings highlight the significance of Pleistocene Forest corridors north of the Andes as the key factor maintaining communication before the separation of the lineages, likely associated with the retraction of this forest. We highlight the absence of any significant differentiation between the disjunct Amazonian and Atlantic Forest populations, at both part of the Cis‐Andean lineage. The phylogeographic profile of A. spadiceus diverges from the patterns observed in other Neotropical birds, which emphasizes the need for further research on the role of the forest corridors of the northern Andes as drivers of diversification, to provide comprehensive insights into the processes that led to the formation of the region's present‐day avian diversity.
The Influence of Migration Timing and Local Conditions on Reproductive Timing in Arctic‐Breeding Birds
Authors: English W. B. , Lagassé B. , Brown S. , Boldenow M. , Burger J. , Casler B. , Dey A. D. , Feigin S. , Freeman S. , Gates H. R. , Iaquinto K. E. , Koch S. , Lamarre J. F. , Lanctot R. B. , Latty C. , Loverti V. , McKinnon L. , Newstead D. , Niles L. , Nol E. , Payer D. , Porter R. , Rausch J. , Saalfeld S. T. , Sanders F. , Senner N. R. , Schulte S. , Sowl K. , Winn B. , Wright L. , Wunder M. B. , Smith P. A. 
Published: January 2025

ABSTRACTFor birds breeding in the Arctic, nest success is affected by the timing of nest initiation, which is partially determined by local conditions such as snow cover. However, conditions during the non‐breeding season can carry over to affect the timing of breeding. We used tracking and breeding data from 248 individuals of 8 species and subspecies of Arctic‐breeding shorebirds to estimate how the timing of nest initiation is related to local conditions like snowmelt phenology versus prior conditions, measured by the timing and speed of migration. Using path analysis, our global model showed that local and prior conditions have similar effect sizes (Standardised Path Coefficients ± SE of 0.44 ± 0.07 and 0.43 ± 0.07 for snowmelt and arrival timing, respectively), suggesting that both influence the timing of breeding and therefore potentially reproductive output. However, the importance of each variable varied across species. Individuals that arrived later to the breeding grounds did not leave the wintering grounds later, but instead took longer to migrate, potentially reflecting differences in flight speed or time spent at stopover sites. We hypothesise that this may be due to reduced habitat quality at some stopover sites or an inability to adjust their departure timing or migration speed to match the advancing spring phenology in the North. Individuals that migrated longer distances also arrived and nested later. Our results highlight the benefits and potential conservation implications of using a full annual cycle approach to assess the factors influencing reproductive timing of birds.