bluuebunny/crossref_metadata_2025_split huggingface dataset:
From intent to impact—The decline of broader impacts throughout an NSF project life cycle
Authors: Woodson Thomas , Boutilier Sophia 
Published: January 2023

Abstract
It is important for funding agencies to evaluate if scientists accomplish their research goals. By comparing a representative sample of National Science Foundation abstracts and project outcome reports (PORs) from 2014 to 2017, this article investigates whether scientists attain the broader impacts they propose. We find that the number of broader impacts proposed in the abstracts is significantly higher than the number of broader impacts reported in the PORs. The trend is common across directorates and type of impact, except when impacts serve advantaged groups. Only the number of broader impacts for advantaged groups increases from the abstract to the POR. Despite the difference between proposed impact and reported impact, our study does not conclude that scientists are delinquent or disingenuous when they propose their research. Rather, we question the capacity of current frameworks to capture the quality of impacts and to weigh the relative importance of impacts that serve marginalized groups versus those that sustain the status quo.
A multidisciplinary lifestyle program for rheumatoid arthritis: the ‘Plants for Joints’ randomized controlled trial
Authors: Walrabenstein Wendy , Wagenaar Carlijn A , van der Leeden Marike , Turkstra Franktien , Twisk Jos W R , Boers Maarten , van Middendorp Henriët , Weijs Peter J M , van Schaardenburg Dirkjan 
Published: January 2023

Abstract

Objective
To determine the effect of a multidisciplinary lifestyle program in patients with RA with low–moderate disease activity.

Methods
In the ‘Plants for Joints’ (PFJ) parallel-arm, assessor-blind randomized controlled trial, patients with RA and 28-joint DAS (DAS28) ≥2.6 and ≤5.1 were randomized to the PFJ or control group. The PFJ group followed a 16-week lifestyle program based on a whole-food plant-based diet, physical activity and stress management. The control group received usual care. Medication was kept stable 3 months before and during the trial whenever possible. We hypothesized that PFJ would lower disease activity (DAS28). Secondary outcomes included anthropometric, metabolic and patient-reported measures. An intention-to-treat analysis with a linear mixed model adjusted for baseline values was used to analyse between-group differences.

Results
Of the 83 people randomized, 77 completed the study. Participants were 92% female with mean (s.d.) age of 55 (12) years, BMI of 26 (4) kg/m2 and mean DAS28 of 3.8 (0.7). After 16 weeks the PFJ group had a mean 0.9-point greater improvement of DAS28 vs the control group (95% CI 0.4, 1.3; P &lt; 0.0001). The PFJ intervention led to greater decreases in body weight (difference –3.9 kg), fat mass (–2.8 kg), waist circumference (–3 cm), HbA1c (–1.3 mmol/mol) and low-density lipoprotein (–0.32 mmol/l), whereas patient-reported outcome measures, blood pressure, glucose and other lipids did not change.

Conclusion
The 16-week PFJ multidisciplinary lifestyle program substantially decreased disease activity and improved metabolic status in people with RA with low–moderate disease activity.

Trial Registration
International Clinical Trials Registry Platform; https://www.who.int/clinical-trials-registry-platform; NL7800.
Fetal–neonatal and maternal outcomes in women with Sjögren syndrome: a population-based registry linkage study
Authors: Chan Tien-Ming , Wu Chiao-En , Yu Han-Hua , Hsiao Chao-yang , Su Tse-Hsuan , Chen Chun-Bing , Chiou Meng-Jiung , Yu Kuang-Hui , Kuo Chang-Fu 
Published: January 2023

Abstract

Objective
To provide better preconceptional and prenatal counselling to patients with sjögren syndrome (SS).

Methods
In total, 2 100 143 pregnancies between 2004 and 2014 were identified in the Taiwan National Health Insurance database and birth registry. The maternal history of SS was ascertained, and data were compared between pregnant women with and without SS. We assessed the odds ratios and 95% CIs of fetal–neonatal and maternal outcomes.

Results
There were 449 pregnancies in women with SS and 2 099 694 pregnancies in women without SS. The risks of still birth [odds ratio (OR) = 2.14, 95% CI = 1.01, 4.55], low birth weight (&lt;2500 g, OR = 2.53, 95% CI = 1.92, 3.33), small for gestational age (OR = 2.03, 95% CI = 1.57, 2.03) and fetal distress (OR = 1.72, 95% CI = 1.2, 2.45) as well as maternal risks of pulmonary oedema (OR = 11.64, 95% CI = 1.62, 83.48), shock (OR = 6.07, 95% CI = 1.51, 24.3) and respiratory distress (OR = 5.61, 95% CI = 1.39, 22.6) were higher in the SS group than in the non-SS group.

Conclusion
Women with SS have significant risks of adverse fetal–neonatal and maternal outcomes and must undergo prenatal counselling to understand the risks involved before conception.
Sulphated penta-galloyl glucopyranoside (SPGG) is glycosaminoglycan mimetic allosteric inhibitor of cathepsin G
Authors: Al-Horani Rami A , Afosah Daniel K , Kar Srabani , Aliter Kholoud F , Mottamal Madhusoodanan 
Published: January 2023

AbstractObjectiveCathepsin G (CatG) is a cationic serine protease with wide substrate specificity. CatG is reported to play a role in several inflammatory pathologies. Thus, we aimed at identifying a potent and allosteric inhibitor of CatG to be used as a platform in further drug development opportunities.MethodsChromogenic substrate hydrolysis assays were used to evaluate the inhibition potency and selectivity of SPGG towards CatG. Salt-dependent studies, Michaelis–Menten kinetics and SDS-PAGE were exploited to decipher the mechanism of CatG inhibition by SPGG. Molecular modelling was also used to identify a plausible binding site.Key findingsSPGG displayed an inhibition potency of 57 nM against CatG, which was substantially selective over other proteases. SPGG protected fibronectin and laminin against CatG-mediated degradation. SPGG reduced VMAX of CatG hydrolysis of a chromogenic substrate without affecting KM, suggesting an allosteric mechanism. Resolution of energy contributions indicated that non-ionic interactions contribute ~91% of binding energy, suggesting a substantial possibility of specific recognition. Molecular modelling indicated that SPGG plausibly binds to an anion-binding sequence of 109SRRVRRNRN117.ConclusionWe present the discovery of SPGG as the first small molecule, potent, allosteric glycosaminoglycan mimetic inhibitor of CatG. SPGG is expected to open a major route to clinically relevant allosteric CatG anti-inflammatory agents.
Polarized Support for Intimate Partner Violence Gun-Related Interventions
Authors: Groggel Anne 
Published: January 2023

Abstract
Social movements pushed to reconceptualize intimate partner violence (IPV) as a social problem deserving of intervention rather than a private family matter. However, little work has examined which interventions the public is likely to support. How and where do personal politics affect perceptions of and responses to a social problem? To address these questions, 739 participants read a victim’s narrative from a court case and indicated their concern for the victim and support for issuing a protection order, prohibiting the abuser from owning a gun, or the victim owning a gun to protect herself. Concern for the victim and support for issuing a protection order was widespread, regardless of political leaning, with minor variations driven by role-taking and attitudes towards IPV. Similarly, support for the victim receiving a protection order was high, with political ideology and political affiliation having no direct effects. While concern increased support for each intervention, it held less explanatory power for gun-related interventions. Instead, political ideology and affiliation shaped support for disarming the abuser or arming the victim. Support for these interventions seemed to filter through a political lens. Thus, one’s personal politics drive divergent intervention attitudes, even when concern for a social problem is shared.
Comparing the Therapeutic Mechanism and Immune Response of Human and Mouse Mesenchymal Stem Cells in Immunocompetent Mice With Acute Liver Failure
Authors: Wang Chang-Hung , Chen Che-Yi , Wang Kai-Hung , Kao An-Pei , Chen Yi-Jou , Lin Pei-Hsuan , Chen Michael , Wu Tung-Yun , Cheng Jing-Jy , Lee Kuan-Der , Chuang Kuo-Hsiang 
Published: January 2023

Abstract
Current mesenchymal stem cell (MSC) research is based on xenotransplantation of human MSCs (hMSCs) in immunodeficient mice and cannot comprehensively predict MSC repair mechanisms and immunomodulatory effects in damaged tissue. This study compared the therapeutic efficacy, mechanisms, and immune response of hMSCs and mouse MSCs (mMSCs) in immunocompetent mice with CCl4-induced acute liver failure. mMSCs maintained F4/80+ hepatic macrophage recruitment into the damaged liver region, increased IL-6-dependent hepatocyte proliferation, and reduced inflammatory TNF-α cytokine secretion. Moreover, mMSCs reduced α-SMA+ myofibroblast activation by lowering TGF-β1 accumulation in damaged liver tissue. In contrast, hMSCs lowered TNF-α and TGF-β1 by reducing the recruitment of F4/80+ hepatic macrophages, which lost the ability to remove debris and induce IL-6 liver regeneration. Finally, hMSCs, but not mMSCs, caused a significant antibody response in immunocompetent mice; therefore, hMSCs are unsuitable for long-term MSC studies. This comparative study provides reference information for further MSC studies of immunocompetent mice.
Effects of Aspergillus oryzae prebiotic on dietary energy and nutrient digestibility of growing pigs
Authors: Zhu Jinlong , Shurson Gerald C , Whitacre Lynsey , Ipharraguerre Ignacio R , Urriola Pedro E 
Published: January 2023

Abstract
The objective of this study was to determine the effects of Aspergillus oryzae prebiotic (AOP) on nutrient digestibility in growing pigs fed high-fiber diets. Eighteen growing barrows (initial body weight = 50.6 ± 4.9 kg) were surgically equipped with a T-cannula at the distal ileum. Corn and soybean meal-based diets were formulated with fiber from cereal grain byproducts corn (distillers dried grains with solubles, DDGS), rice (rice bran, RB), or wheat (wheat middlings, WM) to meet or exceed all nutrient requirements for 50 to 75 kg growing pigs. Three additional diets were formulated to contain 0.05% AOP supplemented at the expense of corn in the DDGS diet (DDGS + AOP), RB diet (RB + AOP), and WM diet (WM + AOP). All diets contained 0.5% of titanium dioxide as an indigestible marker. Pigs were allotted randomly to a triplicated 6 × 2 Youden square design with six diets and two successive periods. Ileal digesta and fecal samples were collected for 2 d after a 21-d adaptation period, and dry matter (DM), gross energy (GE), crude protein (CP), ether extract (EE), neutral detergent fiber (NDF), and ash were analyzed to calculate apparent ileal digestibility (AID) and apparent total tract digestibility (ATTD). Standardized ileal digestibility (SID) of amino acids (AA) was calculated by correcting AID with basal endogenous AA losses from the same set of pigs. Pigs fed the DDGS+AOP diet had greater (P &lt; 0.05) AID of EE compared with those fed the DDGS diet. However, supplementation of AOP did not (P &gt; 0.05) affect AID of GE, DM, CP, NDF, ash or SID of AA of any high-fiber diet. Supplementation of 0.05% AOP increased (P &lt; 0.05) ATTD of DM, GE, CP, NDF, and ash in DDGS, RB, and WM diets. Diet digestible energy was 35 kcal/kg greater (P &lt; 0.05) in pigs fed AOP supplemented diets compared with those fed diets without AOP. In conclusion, supplementation of AOP increased ATTD of nutrients and energy value in high-fiber diets containing DDGS, RB, or WM.
Individual theta-band cortical entrainment to speech in quiet predicts word-in-noise comprehension
Authors: Becker Robert , Hervais-Adelman Alexis 
Published: January 2023

AbstractSpeech elicits brain activity time-locked to its amplitude envelope. The resulting speech-brain synchrony (SBS) is thought to be crucial to speech parsing and comprehension. It has been shown that higher speech-brain coherence is associated with increased speech intelligibility. However, studies depending on the experimental manipulation of speech stimuli do not allow conclusion about the causality of the observed tracking. Here, we investigate whether individual differences in the intrinsic propensity to track the speech envelope when listening to speech-in-quiet is predictive of individual differences in speech-recognition-in-noise, in an independent task. We evaluated the cerebral tracking of speech in source-localized magnetoencephalography, at timescales corresponding to the phrases, words, syllables and phonemes. We found that individual differences in syllabic tracking in right superior temporal gyrus and in left middle temporal gyrus (MTG) were positively associated with recognition accuracy in an independent words-in-noise task. Furthermore, directed connectivity analysis showed that this relationship is partially mediated by top-down connectivity from premotor cortex—associated with speech processing and active sensing in the auditory domain—to left MTG. Thus, the extent of SBS—even during clear speech—reflects an active mechanism of the speech processing system that may confer resilience to noise.
Cryopreserved human precision-cut lung slices provide an immune competent pulmonary test system for “on-demand” use and long-term cultures
Authors: Patel Vivek S , Amin Khalid , Wahab Adam , Marimoutou Méry , Ukishima Lindsey , Alvarez Jose , Battle Kelley , Stucki Andreas O , Clippinger Amy J , Behrsing Holger P 
Published: January 2023

AbstractHuman precision-cut lung slices (hPCLS), considered a highly relevant ex vivo model of the lung, offer native architecture and cells of the lung tissue including respiratory parenchyma, small airways, and immune competent cells. However, the irregular availability of donor lungs has limited the accessibility of this system. As described here, thousands of hPCLS can be created from 1 lung, cryopreserved, and used “on demand” by applying slicing and cryopreservation methodology improvements. Fresh and cryopreserved (∼7 and ∼34 weeks; F&C) hPCLS from 1 donor lung were cultured for up to 29 days and evaluated for biomass, viability, tissue integrity, and inflammatory markers in response to lipopolysaccharide (LPS; 5 µg/ml) and Triton X-100 (TX100; 0.1%) challenge (24 h) at days 1, 8, 15, 22, and 29 following culture initiation. The F&C hPCLS retained biomass, viability, and tissue integrity throughout the 29 days and demonstrated immune responsiveness with up to ∼30-fold LPS-induced cytokine increases. Histologically, more than 70% of normal cytomorphological features were preserved in all groups through day 29. Similar retention of tissue viability and immune responsiveness post cryopreservation (4–6 weeks) and culture (up to 14 days) was observed in hPCLS from additional 3 donor lungs. Banking cryopreserved hPCLS from various donors (and disease states) provides a critical element in researching human-derived pulmonary tissue. The retention of viability and functional responsiveness (≥4 weeks) allows evaluation of long-term, complex endpoints reflecting key events in Adverse Outcome Pathways and positions hPCLS as a valuable human-relevant model for use in regulatory applications.
Arsenic causes distinct gene expression changes in macrophages polarizedin vitrowith either interferon-γ or interleukin-4
Authors: Makhani Kiran , Chiavatti Chris , Negro Silva Luis Fernando , Lemaire Maryse , Bolt Alicia M , De Jay Nicolas , Giles Braeden , Zengin Ayse Nazli , Kleinman Claudia L , Mann Koren K 
Published: January 2023

AbstractArsenic exposure is correlated with atherosclerosis in epidemiological studies and in animal models. We have previously shown that arsenic exposure enhanced the atherosclerotic plaque size, increased the plaque lipid content, and decreased the plaque smooth muscle cell and collagen contents in the apolipoprotein E knockout (apoE−/−) mice. However, the percentage of plaque-resident macrophages, the primary drivers of atherosclerosis remained unchanged. Therefore, we hypothesized that although arsenic does not change the quantity of macrophages, it alters the macrophage transcriptome towards a proatherogenic state. To test this hypothesis, we used bone marrow-derived macrophages, polarized them to either interferon-γ (IFN-ɣ) stimulated, proinflammatory or interleukin-4 (IL-4) stimulated, alternatively activated macrophages in the presence or absence of 0.67 µM (50 ppb) arsenic and performed RNA sequencing. Arsenic exposure altered the gene expression of the macrophages in a subtype-specific manner. Most differentially expressed genes (88%) were altered specifically in either IFN-ɣ- or IL-4-stimulated macrophages, whereas in the remaining 12% of genes that changed in both cell types, did so in opposite directions. In IL-4-stimulated macrophages, arsenic significantly downregulated the genes involved in cholesterol biosynthesis and the chemokines CCL17/CCL22, whereas in IFN-ɣ-stimulated macrophages, the genes associated with the liver X receptor (LXR) pathway were downregulated by arsenic. Using a bone marrow transplant experiment, we validated that the deletion of LXRα from the hematopoietic compartment rescued arsenic-enhanced atherosclerosis in the apoE−/− mouse model. Together, these data suggest that arsenic modulates subtype-specific transcriptomic changes in macrophages and further emphasize the need to define macrophage heterogeneity in atherosclerotic plaques in order to evaluate the proatherogenic role of arsenic.
Boron deficiency mediates plant–insect (Diaphorima citri) interaction by disturbing leaf volatile organic compounds and cell wall functions
Authors: Dong ZhiHao , Liu XiaoDong , Srivastava Anoop Kumar , Tan QiLing , Low Wei , Yan Xiang , Wu SongWei , Sun XueCheng , Hu ChengXiao 
Published: January 2023

Abstract Nutritional enhancement has been reported to effectively relieve infected symptoms of Huanglongbing, one of the most destructive diseases of citrus. However, few studies focused on the role of plant nutrition in citrus plant–vector (Asian citrus psyllid; Diaphorina citri Kuwayama) interactions, which is regarded as an important part to develop an effective management strategy.MethodIn the present study, a hydroponic culture was carried out to evaluate the effects of boron deficiency on psyllid feeding process to decode the molecular/biochemical basis of host–psyllid interaction.ResultsBoron deficiency was observed to play a major role in accelerating the release of volatile organic compounds, especially methyl salicylate, affecting the shikimic acid pathway through an elevated synthesis of shikimic acid, l-phenylalanine, 3-phenylpyruvic acid and salicylic acid. These changes made citrus leaf more attractive to psyllid adults. Meanwhile, boron deficiency evidently decreased the boron concentration of leaf cell wall fractions, thereby, weakened the structural stability by affecting pectin and cellulose formations. A significant decrease of cell wall mechanical strength was observed in boron-deficiency leaf, which could be the critical reasons to reduce piercing and to increase phloem ingestion during psyllid feeding.ConclusionOur study demonstrated that boron deficiency facilitated the feeding behavior of psyllid adults through elevated release of methyl salicylate, coupled with weakened mechanical properties of cell wall.
Spatiotemporal, physiological and transcriptomic dynamics of wild jujube seedlings under saline conditions
Authors: He Aobing , Ma Zhibo , Li Yunfei , Huang Chen , Yong Jean Wan Hong , Huang Jian 
Published: January 2023

Abstract
Soil salinity is a major constraint limiting jujube production in China. Wild jujube (Ziziphus jujuba var. spinosa (Bunge) Hu ex H. F. Chow) is widely used as the rootstock of jujube (Z. jujuba) to overcome the saline conditions. To understand the adaptive mechanism in wild jujube under saline conditions, we combined spatiotemporal and physiological assessments with transcriptomic analysis on wild jujube seedlings undergoing various salt treatments. These salt treatments showed dose and duration effects on biomass, photosynthesis, (K+) and (Na+) accumulation. Salt treatments induced higher levels of salicylic acid in roots and leaves, whereas foliar abscisic acid was also elevated after 8 days. The number of differential expression genes increased with higher doses and also longer exposure of NaCl treatments, with concomitant changes in the enriched Gene Ontology terms that were indicative of altered physiological activities. Gene co-expression network analysis identified the core gene sets associated with salt-induced changes in leaves, stems and roots, respectively. The nitrogen transporters, potassium transporters and a few transcription factors belonging to WRKY/MYB/bHLH families were clustered as the hub genes responding to salt treatments, which were related to elevated nitrogen and K+/Na+. Ectopic overexpression of two WRKY transcription factor genes (ZjWRKY6 and ZjWRKY65) conferred stronger salt-tolerance in Arabidopsis thaliana transformants by enhancing the activities of antioxidant enzymes, decreasing malondialdehyde accumulation and maintaining K+/Na+ homeostasis. This study provided evidence about the spatiotemporal, physiological and transcriptomic dynamics of wild jujube during salt stress and identified potential genes for further research to improve salt tolerance in jujube.
Leaf sodium homeostasis controlled by salt gland is associated with salt tolerance in mangrove plant Avicennia marina
Authors: Guo Zejun , Wei Ming-Yue , Zhong You-Hui , Wu Xuan , Chi Bing-Jie , Li Jing , Li Huan , Zhang Lu-Dan , Wang Xiu-Xiu , Zhu Xue-Yi , Zheng Hai-Lei 
Published: January 2023

Abstract
Avicennia marina, a mangrove plant growing in coastal wetland habitats, is frequently affected by tidal salinity. To understand its salinity tolerance, the seedlings of A. marina were treated with 0, 200, 400 and 600 mM NaCl. We found the whole-plant dry weight and photosynthetic parameters increased at 200 mM NaCl but decreased over 400 mM NaCl. The maximum quantum yield of primary photochemistry (Fv/Fm) significantly decreased at 600 mM NaCl. Transmission electron microscopy observations showed high salinity caused the reduction in starch grain size, swelling of the thylakoids and separation of the granal stacks, and even destruction of the envelope. In addition, the dense protoplasm and abundant mitochondria in the secretory and stalk cells, and abundant plasmodesmata between salt gland cells were observed in the salt glands of the adaxial epidermis. At all salinities, Na+ content was higher in leaves than in stems and roots; however, Na+ content increased in the roots while it remained at a constant level in the leaves over 400 mM NaCl treatment, due to salt secretion from the salt glands. As a result, salt crystals on the leaf adaxial surface increased with salinity. On the other hand, salt treatment increased Na+ and K+ efflux and decreased H+ efflux from the salt glands by the non-invasive micro-test technology, although Na+ efflux reached the maximum at 400 mM NaCl. Further real-time quantitative PCR analysis indicated that the expression of Na+/H+ antiporter (SOS1 and NHX1), H+-ATPase (AHA1 and VHA-c1) and K+ channel (AKT1, HAK5 and GORK) were up-regulated, and only the only Na+ inward transporter (HKT1) was down-regulated in the salt glands enriched adaxial epidermis of the leaves under 400 mM NaCl treatment. In conclusion, salinity below 200 mM NaCl was beneficial to the growth of A. marina, and below 400 mM, the salt glands could excrete Na+ effectively, thus improving its salt tolerance.