bluuebunny/crossref_metadata_2025_split huggingface dataset:
Donkey semen cryopreservation: Alternatives with permeable, non‐permeable cryoprotectants and seminal plasma
Authors: Montoya Páez Juan David , Úsuga Suarez Alexandra , Restrepo Betancur Giovanni 
Published: December 2022

AbstractCryopreservation of semen is an important technique to preserve genetic material. Yet, pregnancy rates in jennies after artificial insemination with frozen–thawed donkey semen are poor. This condition has been attributed to the impact of permeable cryoprotectants, that could cause high post‐breeding endometritis. Removal of seminal plasma (SP) prior to semen freezing process is another contributing factor. SP is involved in a multitude of sperm functions and events preceding fertilization and has a mediating effect of sperm capacitation and postcoital uterine inflammatory response. The aim of this study was to evaluate different alternatives in donkey semen cryopreservation with permeable, non‐permeable cryoprotectants, BSA and SP. Thirty ejaculates from 10 donkeys were cryopreserved with different combinations of dimethylformamide (DMF, 5%), sucrose (SUC, 200 mM) and homologous SP (10%): DMF (T1), DMF/SP (T2), SUC/BSA (T3), SUC/BSA/SP (T4), DMF/SUC/BSA (T5), DMF/SUC/BSA/SP (T6), DMF/BSA (T7) and DMF/BSA/SP (T8). After thawing, sperm motility and kinetics were assessed by computerized semen analysis. Sperm vitality (SV) was evaluated by fluorescence microscopy, functional membrane integrity (FMI) by the HOST test, abnormal morphology by eosin‐nigrosin staining and sperm membrane stability by flow cytometry. For statistical analysis, sperm quality indexes (SQi) were obtained, general linear models were carried out and mean comparisons were made by the Tukey test. T1, T2, T5, T6, and T7 had higher and equivalent results for motility, most kinetic parameters and function membrane integrity. Cryopreservation of donkey semen without permeable cryoprotectant (T3 and T4) showed a reduction in motility, kinetics, SV, FMI and SQi. T5 showed a reduction in progressive motility, sperm velocities, IMF and SQi compared to other DMF treatments. T6 and T8 achieved higher SQi values compared to T1, but they were not different compared to T2 and T7. T1 had a smaller sperm population with low‐M540 compared to T3. It is concluded that the use of permeable cryoprotectant is essential to achieve higher post‐thaw quality of donkey semen. In addition, the combined use of BSA, SUC and/or PS may provide additional sperm protection compared to the individual use of DMF.
Registered nurses' efforts to ensure safety for home‐dwelling older patients
Authors: Lindberg Catharina , Fock Jenni , Nilsen Per , Schildmeijer Kristina 
Published: December 2022

AbstractBackgroundThe international development of health care, an ageing population and rapid technical development mean that more care is being performed in patient homes. This care environment is often unpredictable and involves both formal and informal caregivers, making it potentially unsafe. There is sparse knowledge about how patient safety is protected in home health care in Sweden and how registered nurses work to prevent risks and promote safe care.AimThe aim of the study was to explore registered nurses' efforts to reduce perceived risks for home‐dwelling older patients and ensure safe home health care.MethodWe used a qualitative design with individual interviews with 13 registered nurses working in municipalities in southeast Sweden. The narratives were analysed with inductive content analysis.FindingsThe findings showed that the registered nurses tried to secure a safe care environment and took an active role in care, striving to stay one step ahead of the patient. These three types of efforts are likely interdependent, suggesting they are all needed to reduce perceived risks for home‐dwelling older patients and ensure patient safety in home health care.ConclusionsIt is a challenge for registered nurses to maintain patient safety when performing care in patient homes. Continuity of care is required and must be based not only on self‐reliance among registered nurses but also on trusting relationships with patients, next of kin, colleagues and other personnel, as well as on the development of organisational conditions adapted to patient needs.
Transcriptome sequencing reveals two subtypes of cortisol‐secreting adrenocortical tumours in dogs and identifies CYP26B1 as a potential new therapeutic target
Authors: Sanders Karin , Kooistra Hans S. , van den Heuvel Marieke , Mokry Michal , Grinwis Guy C. M. , van den Dungen Noortje A. M. , van Steenbeek Frank G. , Galac Sara 
Published: December 2022

AbstractCushing's syndrome (CS) is a serious endocrine disorder that is relatively common in dogs, but rare in humans. In ~15%–20% of cases, CS is caused by a cortisol‐secreting adrenocortical tumour (csACT). To identify differentially expressed genes that can improve prognostic predictions after surgery and represent novel treatment targets, we performed RNA sequencing on csACTs (n = 48) and normal adrenal cortices (NACs; n = 10) of dogs. A gene was declared differentially expressed when the adjusted p‐value was <.05 and the log2 fold change was >2 or < −2. Between NACs and csACTs, 98 genes were differentially expressed. Based on the principal component analysis (PCA) the csACTs were separated in two groups, of which Group 1 had significantly better survival after adrenalectomy (p = .002) than Group 2. Between csACT Group G1 and Group 2, 77 genes were differentially expressed. One of these, cytochrome P450 26B1 (CYP26B1), was significantly associated with survival in both our canine csACTs and in a publicly available data set of 33 human cortisol‐secreting adrenocortical carcinomas. In the validation cohort, CYP26B1 was also expressed significantly higher (p = .012) in canine csACTs compared with NACs. In future studies it would be interesting to determine whether CYP26B1 inhibitors could inhibit csACT growth in both dogs and humans.
Efficacy and safety of abatacept to treat active birdshot uveitis: a prospective open label interventional proof-of-concept trial
Authors: Schauwvlieghe PP , Van Calster Joachim , Herbort Carl Peter , Kestelyn Philippe A , de Vlam Kurt 
Published: December 2022

Background/aimsBirdshot uveitis (BU) is a chronic autoimmune posterior uveitis, mostly affecting middle-aged Caucasians. There is a strong association with HLA-A29 and T-cell activation. Safety and efficacy of abatacept, an inhibitor of T-cell costimulation, is tested in active BU.MethodsFifteen patients with active BU were treated with monotherapy of weekly subcutaneous injections of abatacept 125 mg/mL. Time-to-treatment failure was evaluated as a primary outcome. The secondary objective was to evaluate the utility of different outcome measures to monitor disease activity. Safety was evaluated by adverse event reporting and serial blood analyses.ResultsAt the year-1 endpoint, there was significant improvement in vitreous haze grade (p=0.0014), central choroidal thickness (CCT) (p=0.0011), Fluorescein Angiography (FA) Score (p=0.0014), Indocyanine Green Angiography (ICGA) Score (p<0.001) and total dual FA-ICGA Score (p<0.001). Best corrected visual acuity (BCVA) (p=0.8354) and central retinal thickness (CRT) (p=0.3549) did not change significantly. There were no serious adverse events reported. In total, 4 out of 15 patients left the trial during year 1 of whom 2 experienced treatment failure.ConclusionsAbatacept is very efficacious to treat both retinal vasculitis and choroiditis in patients with BU and is well tolerated. BCVA and CRT are inadequate to monitor disease activity. On the other hand, CCT is a promising non-invasive tool to detect treatment response in early active BU and dual FA-ICGA Score is very helpful to evaluate retinal vasculitis and choroiditis quantitatively.Trial registration numberNCT03871361.
Availability and prioritisation of COVID-19 vaccines among patients with advanced chronic kidney disease and kidney failure during the height of the pandemic: a global survey by the International Society of Nephrology
Authors: Wijewickrama Eranga S , Abdul Hafidz Muhammad Iqbal , Robinson Bruce M , Johnson David W , Liew Adrian , Dreyer Gavin , Caskey Fergus J , Bello Aminu K , Zaidi Deenaz , Damster Sandrine , Salaro Silvia , Luyckx Valerie Ann , Bajpai Divya 
Published: December 2022

ObjectivePatients with advanced chronic kidney disease (CKD) or kidney failure receiving replacement therapy (KFRT) are highly vulnerable to COVID-19 infection, morbidity and mortality. Vaccination is effective, but access differs around the world. We aimed to ascertain the availability, readiness and prioritisation of COVID-19 vaccines for this group of patients globally.Setting and participantsCollaborators from the International Society of Nephrology (ISN), Dialysis Outcomes and Practice Patterns Study and ISN-Global Kidney Health Atlas developed an online survey that was administered electronically to key nephrology leaders in 174 countries between 2 July and 4 August 2021.ResultsSurvey responses were received from 99 of 174 countries from all 10 ISN regions, among which 88/174 (50%) were complete. At least one vaccine was available in 96/99 (97%) countries. In 71% of the countries surveyed, patients on dialysis were prioritised for vaccination, followed by patients living with a kidney transplant (KT) (62%) and stage 4/5 CKD (51%). Healthcare workers were the most common high priority group for vaccination. At least 50% of patients receiving in-centre haemodialysis, peritoneal dialysis or KT were estimated to have completed vaccination at the time of the survey in 55%, 64% and 51% of countries, respectively. At least 50% of patients in all three patient groups had been vaccinated in >70% of high-income countries and in 100% of respondent countries in Western Europe.The most common barriers to vaccination of patients were vaccine hesitancy (74%), vaccine shortages (61%) and mass vaccine distribution challenges (48%). These were reported more in low-income and lower middle-income countries compared with high-income countries.ConclusionPatients with advanced CKD or KFRT were prioritised in COVID-19 vaccination in most countries. Multiple barriers led to substantial variability in the successful achievement of COVID-19 vaccination across the world, with high-income countries achieving the most access and success.
Collaboration and coordination of health and care services for older people with dementia by multidisciplinary health and care providers: a scoping review protocol
Authors: Österholm Johannes , Nedlund Ann-Charlotte , Larsson Ranada Åsa 
Published: December 2022

IntroductionCollaboration and coordination of health and care services are key to catering for the diverse needs of a growing population of older people with dementia. When multidisciplinary health and care providers work together, they have the possibility to use resources in a fair, accurate and effective way and thereby do the right thing, at the right time, for the right individual. The aim of this scoping review is to map how different care-providing agencies collaborate and coordinate health and care services for older people with dementia.Methods and analysisA scoping review will be carried out following the proposed methodology by Joanna Briggs Institute and reported according to the Preferred Reporting Items for Systematic Reviews and Meta-Analyses-Scoping Review Extension guidelines. Systematic searches will be carried out in scientific databases. Studies published within the last 10 years will be included based on certain eligibility criteria. All included studies will be critically appraised using the Research Pyramid. Data from included studies will be charted and subjected to content analysis.Ethics and disseminationEthics approval is not required for scoping reviews. The dissemination of findings will be conducted through conference presentations and publication in international scientific journals.
Factors influencing the integration of self-management in daily life routines in chronic conditions: a scoping review of qualitative evidence
Authors: Qama Enxhi , Rubinelli Sara , Diviani Nicola 
Published: December 2022

ObjectiveSelf-management of chronic diseases is regarded as dynamic experience which is always evolving and that requires constant adjustment. As unexpected and new shifts in diseases occur, patients tend to abandon acquired behaviours calling into question their sustainability over time. Developing a daily self-management routine as a response to lifestyle changes is considered to facilitate self-management performance. However, fitting self-management recommendations in one’s daily life activities is a constant challenge. In this review, we describe the performance of self-management routines within daily settings in people living with chronic conditions with the aim of identifying factors that challenge its integration in daily life.DesignScoping review.Data sourcesWe searched PubMed, Web of Science, CINAHL and PsycINFO on February 2022.Eligibility criteriaWe included qualitative studies on self-management experience, in English, with adult participants, original and peer-reviewed, and depicting the performance of self-management activities in one’s own environment.Data extraction and synthesisTwo reviewers independently screened titles and abstracts. After agreement, one reviewer screened the full text of relevant articles and extracted the data. The data were synthesised and analysed thematically. PRISMA Extension for Scoping Reviews checklist was used for reporting the steps.ResultsTwenty-two studies were included. The thematic analysis brought up two overreaching themes. The first one is the Environment support with three subthemes: family and cultural norms; health professionals and guiding communication; and society and disease perceptions. The second theme is comprehension gap with two subthemes: reading the body and applying information.ConclusionsThe integration of self-management requirements in a daily routine is affected by the patients’ inability to apply disease knowledge in different context and by the challenge of understanding body symptoms and predicting body reactions in advance.
Contribution of sexual health services to hepatitis B detection and control (Netherlands, 2008–2016)
Authors: Raven Stijn , Hautvast Jeannine , Yiek Wing-Kee , Veldhuijzen Irene , van Steenbergen Jim , van Aar Fleur , Hoebe Christian J P A 
Published: December 2022

ObjectivesCase finding is one of the priority actions to reduce the disease burden of chronic hepatitis B (CHB). We estimated the contribution of CHB case finding at sexual health centres (SHCs) to the total national number of newly diagnosed CHB cases in the Netherlands and determined the characteristics of CHB cases detected at SHCs.MethodsThis observational study used surveillance data from all outpatient SHCs in the Netherlands (SOAP database) and the number of CHB from national notification data (Osiris) from 2008 to 2016. The proportion of CHB notifications (hepatitis B surface antigen positive serology) detected at SHCs was calculated. SHC consultations without hepatitis B virus (HBV) testing (n=669 308), with acute hepatitis B diagnosis (n=73), with HBV vaccination only (n=182) or an inconclusive hepatitis B diagnosis (n=24) were excluded. Univariable and multivariable logistic regression analyses were performed, stratified by gender and sexual preference, to analyse patient characteristics associated with CHB.ResultsDuring the study period, 12 149 CHB cases were notified. 405 646 SHC consultations were included in the analysis and 1452 CHB cases (0.4%) were detected at SHCs. The proportion of CHB cases detected at SHCs in relation to the national notified number ranged between 12.4% (200 of 1613) in 2008 and 10.8% (106 of 980) in 2016. 87% of CHB cases were among first-generation migrants (FGMs) originating from high endemic countries for sexually transmitted infections or men who have sex with men (MSM). In multivariable analysis, an older age category, migration background and being a commercial sex worker (CSW) were associated with CHB in all stratified analyses.ConclusionsThe contribution of SHCs is relevant to case finding of CHB in the Netherlands. SHCs should therefore be considered as an important health setting to screen for HBV in high-risk groups, especially among MSM, CSW and FGM, to achieve a reduction in the HBV-related disease burden.
New benchmark models for heavy neutral lepton searches
Authors: Drewes M. , Klarić J. , López-Pavón J. 
Published: December 2022

AbstractThe sensitivity of direct searches for heavy neutral leptons (HNLs) in accelerator-based experiments depends strongly on the particles properties. Commonly used benchmark scenarios are important to ensure comparability and consistency between experimental searches, re-interpretations, and sensitivity studies for different facilities. In models where the HNLs are primarily produced and decay through the weak interaction, benchmarks are in particular defined by fixing the relative strengths of their mixing with SM neutrinos of different flavours, and the interpretation of experimental data is known to strongly depend on those ratios. The commonly used benchmarks in which a single HNL flavour exclusively interacts with one Standard Model generation do not reflect what is found in realistic neutrino mass models. We identify two additional benchmarks for accelerator-based direct HNL searches, which we primarily select based on the requirement to provide a better approximation for the phenomenology of realistic neutrino mass models in view of present and future neutrino oscillation data.
ДЕЯКІ АСПЕКТИ ВИКОРИСТАННЯ НЕНАТЯЖНОГО СПОСОБУ ХІРУРГІЧНОГО ЛІКУВАННЯ ПАХОВИХ ГРИЖ
Authors: Бенедикт В. В. , Продан А. М. , Яцук К. Ю. 
Published: December 2022

РЕЗЮМЕ. У статті наведено результати використання нового ефективного ненатяжного способу хірургічного лікування пахових гриж із застосуванням синтетичного імплантата для зменшення вірогідності виникнення рецидиву грижі і прискорення трудової та соціальної реабілітації хворих після операції.
Мета – створення нового ефективного ненатяжного способу хірургічного лікування пахових гриж із застосуванням синтетичного імплантата для зменшення вірогідності виникнення рецидиву грижі і прискорення трудової та соціальної реабілітації хворих після операції.
Матеріал і методи. Проведено комплексне обстеження 34 хворих на пахову грижу з використанням загальноприйнятих методів, а також сонографічне дослідження передньої черевної стінки до операції і в різні строки після хірургічного лікування.
Результати. При визначенні інтенсивності больового синдрому за ВАШ отримано результати: до 3-х годин післяопераційного періоду показник склав (22,9±2,1) мм; через 3 – 6 годин – (28,9±2,5) мм (р<0,05), що не вимагало введення наркотичних і навіть ненаркотичних знеболювальних. Це свідчить про достовірно менший ступінь інтенсивності больового синдрому в пацієнтів після використання герніопластики за Desarda. За даними УЗД, набряку тканин, сером, інфільтратів зони шва не виявлено. Післяопераційна рана загоювалася первинним натягом у всіх пацієнтів, хворих на другу – четверту добу після операції виписували з хірургічного стаціонару на амбулаторне лікування. Через 18–36 місяців було обстежено 24 пацієнти. У жодного з них не було діагностовано рецидиву грижі.
Висновки. Використання для пластики пахового каналу як власних тканин, так і синтетичного імпланта, посилює механічну опірність новосформованої задньої стінки пахового каналу і дає змогу досягти підвищення ефективності хірургічного лікування пахових гриж та знизити ризик їх рецидиву.
ВПЛИВ L-ОРНІТИНУ L-АСПАРТАТУ НА ВМІСТ TNF-A ТА TGF-ß У ПЕЧІНЦІ ПРИ ЕКСПЕРИМЕНТАЛЬНОМУ ЦИРОЗІ НА ФОНІ БЛОКАДИ СИНТЕЗУ ОКСИДУ АЗОТУ ТА ПОЛІАМІНІВ
Authors: Дацко В. А. , Олещук О. М. 
Published: December 2022

РЕЗЮМЕ. Мета – провести імуногістохімічне дослідження вмісту трансформуючого фактора росту β (TGF-β) та фактора некрозу пухлин α (TNF-α) в печінці та дослідити структуру органа при експериментальному CCL4--індукованому цирозі й ефективність застосування L-орнітину L-аспартату за умови блокади синтезу оксиду азоту та поліамінів.
Матеріал і методи. Експериментальний цироз печінки моделювали шляхом перорального введення білим щурам лінії Вістар 50 % олійного розчину CCL4 впродовж 12 тижнів. З метою корекції використовували L-орнітину L-аспартат (LOLA) в дозі 200 мг/кг, а також LOLA у комбінації із неселективним блокатором синтезу азоту L-NAME у дозі 10 мг/кг та блокатором синтезу поліамінів DFMO у дозі 25 мг/кг. Проводили гістологічне та імуногістохімічне дослідження печінки тварин із визначенням фактора росту (TGF-β) та фактора некрозу пухлин (TNF-α).
Результати. Виявили формування CCL4 індукованого цирозу із формуванням вираженого фіброзу за ходом портальних трактів, дистрофічними та дистрофічно-некротичними змінами в гепатоцитах, значною лімфо-гістіоцитарною інфільтрацією фіброзних полів, появою TNF-α та TGF-β факторів на мембранах клітин переважно в просвітах синусоїдів. Спостерігається достовірне збільшення даних показників при впливі неселективного блокатора NO-синтаз L-NAME та інгібітора метаболізму поліамінів DFMO.
Висновки. Застосування LOLA при експериментальному цирозі печінки різко знижує активацію TNF-α та TGF-β на мембранах клітин в просвітах синусоїдів та частково знижує кількість лімфогістіоцитарної інфільтрації переважно в паренхімі печінки, покращує структуру печінки. Блокада синтезу оксиду азоту, а особливо поліамінів, на фоні застосування LOLA нівелює позитивний вплив останнього, що вказує на важливу роль синтезу NO та поліамінів у механізмі дії LOLA.
ЯКІСТЬ ЖИТТЯ ТА ФУНКЦІОНАЛЬНИЙ СТАН ПАЦІЄНТІВ ЗІ СТАБІЛЬНОЮ ІШЕМІЧНОЮ ХВОРОБОЮ СЕРЦЯ В ПОЄДНАННІ З ХРОНІЧНИМ ОБСТРУКТИВНИМ ЗАХВОРЮВАННЯМ ЛЕГЕНЬ
Authors: Князєва О. В. 
Published: December 2022

Резюме. Мета дослідження – вивчити компоненти якості життя (ЯЖ) та їх взаємозв’язок з функціональним станом пацієнтів зі стабільною ішемічною хворобою серця (ІХС) в поєднанні з хронічним обструктивним захворюванням легень (ХОЗЛ).
Матеріал і методи. Основну групу (група 1) склали 108 чоловіків зі стабільною ІХС та ХОЗЛ. В якості груп порівняння обстежено 30 чоловіків зі стабільною ІХС (група 2) та 30 чоловіків з ХОЗЛ (група 3). Контрольну групу склали 30 чоловіків без ІХС та хвороб органів дихання. Групи зіставні за віком. Визначення ЯЖ проводили за неспецифічним опитувальником «The 36-Item Short Form Health Survey» (SF-36), а у пацієнтів груп 1 та 3 також за специфічним респіраторним опитувальником Госпіталю Святого Георгія (SGRQ). Толерантність до фізичного навантаження оцінювали за кардіопульмональним тестом, що включав дистанцію 6-хвилинної ходьби (6ХТзХ), пульсоксиметрію з розрахунком рівня десатурації, реєстрацію електрокардіограми та артеріального тиску до і після навантаження.
Результати. За опитувальником SF-36, в групі 1 виявлено достовірне зниження ЯЖ, пов’язане із фізичним, рольовим та соціальним функціонуванням, загальною оцінкою стану здоров’я, життєвою активністю та психічним здоров’ям, порівняно з групами 2 та 3. Виявлені кореляційні зв’язки між показниками SF-36 та клініко-функціональними характеристиками ХОЗЛ, що свідчить про обтяження ЯЖ пацієнтів з ІХС за рахунок коморбідного ХОЗЛ. Пацієнти групи 1 мали достовірно меншу дистанцію 6ХТзХ, достовірно більший рівень десатурації, порівняно з групами 2 та 3. Встановлено кореляційні зв’язки показників кардіопульмонального тесту зі складовими фізичного та психічного компонентів ЯЖ.
Висновки. Пацієнти зі стабільною ІХС в поєднанні з ХОЗЛ мали достовірно гірші показники доменів фізичного та психічного компонентів ЯЖ, ніж пацієнти з ізольованими ІХС та ХОЗЛ. Зниження толерантності до фізичного навантаження у цих пацієнтів пов’язано з гіршими показниками фізичного та окремих доменів психічного компонента ЯЖ.