bluuebunny/crossref_metadata_2025_split huggingface dataset:
Effect of municipal biowaste derived biostimulant on nitrogen fate in the plant-soil system during lettuce cultivation
Authors: Fragalà Ferdinando , Puglisi Ivana , Padoan Elio , Montoneri Enzo , Stevanato Piergiorgio , Gomez Josè Maria , Herrero Natalia , La Bella Emanuele , Salvagno Erika , Baglieri Andrea 
Published: May 2023

AbstractA main concern of agriculture is to improve plant nutrient efficiency to enhance crop yield and quality, and at the same time to decrease the environmental impact caused by the lixiviation of excess N fertilizer application. The aim of this study was to evaluate the potential use of biopolymers (BPs), obtained by alkaline hydrolysis of the solid anaerobic digestate of municipal biowastes, in order to face up these main concerns of agriculture. The experimental trials involved the application of BPs (at 50 and 150 kg/ha) alone or mixed with different amounts (100%, 60% and 0%) of mineral fertilizer (MF). Three different controls were routinely included in the experimental trials (MF 100%, 60% and 0%). The effect of BPs on lettuce was evaluated by monitoring growth parameters (fresh and dry weights of shoot and root, nitrogen use efficiency), and the N-flux in plant-soil system, taking into account the nitrate leached due to over irrigation events. The activities of enzymes involved in the nitrogen uptake (nitrate reductase, glutamate synthase and glutamine synthase), and the nitrogen form accumulated in the plant tissues (total N, protein and NO3−) were evaluated. The results show that the application to the soil of 150 kg/ha BPs allows to increase lettuce growth and nitrogen use efficiency, trough stimulation of N-metabolism and accumulation of proteins, and hence to reduce the use of MF by 40%, thus decreasing the nitrate leaching. These findings suggest that the use of BPs as biostimulant greatly contributes to reduce the consumption of mineral fertilizers, and to mitigate the environmental impact caused by nutrients leaching, according to European common agricultural policy, that encourages R&D of new bioproducts for sustainable eco-friendly agriculture.
Direct detection and identification of periprosthetic joint infection pathogens by metagenomic next-generation sequencing
Authors: Hao Linjie , Wen Pengfei , Song Wei , Zhang Binfei , Wu Yanjie , Zhang Yumin , Ma Tao , Qiu Yusheng 
Published: May 2023

AbstractThis study assessed the application of metagenomic next-generation sequencing in pathogen detection of periprosthetic joint infections. A total of 95 cases who previously had undergone hip and knee replacement undergoing revision from January 2018 to January 2021 were included in this study. Specimens of synovial fluid and deep-tissue were collected for culture and metagenomic next-generation sequencing, and patients were retrospectively categorized as infected or aseptic using the Musculoskeletal Infection Society criteria after revision surgery. The sensitivity, specificity, positive and negative predictive values were compared. A total of 36 cases had positive culture results and 59 cases had positive metagenomic next-generation sequencing results. Culture was positive in 34 infected cases (58.6%) and 2 aseptic cases (5.4%). Metagenomic next-generation sequencing was positive in 55 infected cases (94.8%) and 4 aseptic cases (10.8%). Five cases diagnosed with infection had other potential pathogens detected by metagenomic next-generation sequencing. Among the 24 culture-negative periprosthetic joint infections, metagenomic next-generation sequencing was able to identify potential pathogens in 21 cases (87.5%). From sampling to reporting, the average time needed for culture was 5.2 (95% CI 3.1–7.3) days, while that for metagenomic next-generation sequencing was 1.3 (95% CI 0.9–1.7) days. Metagenomic next-generation sequencing is more advantageous in pathogen detection of periprosthetic joint infection after total joint replacement, especially in patients with multiple infections or negative culture results.
The contribution of industrial emissions to ozone pollution: identified using ozone formation path tracing approach
Authors: Zhan Junlei , Ma Wei , Song Boying , Wang Zongcheng , Bao Xiaolei , Xie Hong-Bin , Chu Biwu , He Hong , Jiang Tao , Liu Yongchun 
Published: May 2023

AbstractWintertime meteorological conditions are usually unfavorable for ozone (O3) formation due to weak solar irradiation and low temperature. Here, we observed a prominent wintertime O3 pollution event in Shijiazhuang (SJZ) during the Chinese New Year (CNY) in 2021. Meteorological results found that the sudden change in the air pressure field, leading to the wind changing from northwest before CNY to southwest during CNY, promotes the accumulation of air pollutants from southwest neighbor areas of SJZ and greatly inhibits the diffusion and dilution of local pollutants. The photochemical regime of O3 formation is limited by volatile organic compounds (VOCs), suggesting that VOCs play an important role in O3 formation. With the developed O3 formation path tracing (OFPT) approach for O3 source apportionment, it has been found that highly reactive species, such as ethene, propene, toluene, and xylene, are key contributors to O3 production, resulting in the mean O3 production rate (PO3) during CNY being 3.7 times higher than that before and after CNY. Industrial combustion has been identified as the largest source of the PO3 (2.6 ± 2.2 ppbv h−1), with the biggest increment (4.8 times) during CNY compared to the periods before and after CNY. Strict control measures in the industry should be implemented for O3 pollution control in SJZ. Our results also demonstrate that the OFPT approach, which accounts for the dynamic variations of atmospheric composition and meteorological conditions, is effective for O3 source apportionment and can also well capture the O3 production capacity of different sources compared with the maximum incremental reactivity (MIR) method.
In situ-formable, dynamic crosslinked poly(ethylene glycol) carrier for localized adeno-associated virus infection and reduced off-target effects
Authors: Kato Motoi , Ishikawa Shohei , Shen Qi , Du Zening , Katashima Takuya , Naito Mitsuru , Numahata Takao , Okazaki Mutsumi , Sakai Takamasa , Kurita Masakazu 
Published: May 2023

AbstractThe adeno-associated virus (AAV) is a potent vector for in vivo gene transduction and local therapeutic applications of AAVs, such as for skin ulcers, are expected. Localization of gene expression is important for the safety and efficiency of genetic therapies. We hypothesized that gene expression could be localized by designing biomaterials using poly(ethylene glycol) (PEG) as a carrier. Here we show one of the designed PEG carriers effectively localized gene expression on the ulcer surface and reduced off-target effects in the deep skin layer and the liver, as a representative organ to assess distant off-target effects, using a mouse skin ulcer model. The dissolution dynamics resulted in localization of the AAV gene transduction. The designed PEG carrier may be useful for in vivo gene therapies using AAVs, especially for localized expression.
Singlet oxygen-induced signalling depends on the metabolic status of the Chlamydomonas reinhardtii cell
Authors: Youssef Waeil Al , Feil Regina , Saint-Sorny Maureen , Johnson Xenie , Lunn John E. , Grimm Bernhard , Brzezowski Pawel 
Published: May 2023

AbstractUsing a mutant screen, we identified trehalose 6-phosphate phosphatase 1 (TSPP1) as a functional enzyme dephosphorylating trehalose 6-phosphate (Tre6P) to trehalose inChlamydomonas reinhardtii. Thetspp1knock-out results in reprogramming of the cell metabolism via altered transcriptome. As a secondary effect,tspp1also shows impairment in1O2-induced chloroplast retrograde signalling. From transcriptomic analysis and metabolite profiling, we conclude that accumulation or deficiency of certain metabolites directly affect1O2-signalling.1O2-inducibleGLUTATHIONE PEROXIDASE 5(GPX5) gene expression is suppressed by increased content of fumarate and 2-oxoglutarate, intermediates in the tricarboxylic acid cycle (TCA cycle) in mitochondria and dicarboxylate metabolism in the cytosol, but also myo-inositol, involved in inositol phosphate metabolism and phosphatidylinositol signalling system. Application of another TCA cycle intermediate, aconitate, recovers1O2-signalling andGPX5expression in otherwise aconitate-deficienttspp1. Genes encoding known essential components of chloroplast-to-nucleus1O2-signalling, PSBP2, MBS, and SAK1, show decreased transcript levels intspp1, which also can be rescued by exogenous application of aconitate. We demonstrate that chloroplast retrograde signalling involving1O2depends on mitochondrial and cytosolic processes and that the metabolic status of the cell determines the response to1O2.
Copy number alteration features in pan-cancer homologous recombination deficiency prediction and biology
Authors: Yao Huizi , Li Huimin , Wang Jinyu , Wu Tao , Ning Wei , Diao Kaixuan , Wu Chenxu , Wang Guangshuai , Tao Ziyu , Zhao Xiangyu , Chen Jing , Sun Xiaoqin , Liu Xue-Song 
Published: May 2023

AbstractHomologous recombination deficiency (HRD) renders cancer cells vulnerable to unrepaired double-strand breaks and is an important therapeutic target as exemplified by the clinical efficacy of poly ADP-ribose polymerase (PARP) inhibitors as well as the platinum chemotherapy drugs applied to HRD patients. However, it remains a challenge to predict HRD status precisely and economically. Copy number alteration (CNA), as a pervasive trait of human cancers, can be extracted from a variety of data sources, including whole genome sequencing (WGS), SNP array, and panel sequencing, and thus can be easily applied clinically. Here we systematically evaluate the predictive performance of various CNA features and signatures in HRD prediction and build a gradient boosting machine model (HRDCNA) for pan-cancer HRD prediction based on these CNA features. CNA features BP10MB[1] (The number of breakpoints per 10MB of DNA is 1) and SS[ > 7 & <=8] (The log10-based size of segments is greater than 7 and less than or equal to 8) are identified as the most important features in HRD prediction. HRDCNAsuggests the biallelic inactivation ofBRCA1,BRCA2,PALB2,RAD51C,RAD51D, andBARD1as the major genetic basis for human HRD, and may also be applied to effectively validate the pathogenicity of BRCA1/2 variants of uncertain significance (VUS). Together, this study provides a robust tool for cost-effective HRD prediction and also demonstrates the applicability of CNA features and signatures in cancer precision medicine.
The earliest Ethiopian wolf: implications for the species evolution and its future survival
Authors: Martínez-Navarro Bienvenido , Gossa Tegenu , Carotenuto Francesco , Bartolini-Lucenti Saverio , Palmqvist Paul , Asrat Asfawossen , Figueirido Borja , Rook Lorenzo , Niespolo Elizabeth M. , Renne Paul R. , Herzlinger Gadi , Hovers Erella 
Published: May 2023

AbstractIn 2017, a hemimandible (MW5-B208), corresponding to the Ethiopian wolf (Canis simensis), was found in a stratigraphically-controlled and radio-isotopically-dated sequence of the Melka Wakena paleoanthropological site-complex, on the Southeastern Ethiopian Highlands, ~ 2300 m above sea level. The specimen is the first and unique Pleistocene fossil of this species. Our data provide an unambiguous minimum age of 1.6–1.4 Ma for the species’ presence in Africa and constitutes the first empirical evidence that supports molecular interpretations. Currently,C. simensisis one of the most endangered carnivore species of Africa. Bioclimate niche modeling applied to the time frame indicated by the fossil suggests that the lineage of the Ethiopian wolf faced severe survival challenges in the past, with consecutive drastic geographic range contractions during warmer periods. These models help to describe future scenarios for the survival of the species. Projections ranging from most pessimistic to most optimistic future climatic scenarios indicate significant reduction of the already-deteriorating territories suitable for the Ethiopian Wolf, increasing the threat to the specie’s future survival. Additionally, the recovery of the Melka Wakena fossil underscores the importance of work outside the East African Rift System in research of early human origins and associated biodiversity on the African continent.
Comparative photometric analysis of the Reiner Gamma swirl and Chang’e 5 landing site
Authors: Hess M. , Wöhler C. , Qiao L. , Bhatt M. 
Published: May 2023

Context.Lunar swirls are bright albedo features only found on the Moon that are still not entirely understood. It is commonly accepted that reduced space weathering plays a role in explaining the origins of lunar swirls because the local magnetic fields that are typically associated with these albedo anomalies are effective in reducing the solar wind influx. However, additional processes are required to fully explain the spectral, photometric, and polarimetric properties of the swirls.Aims.In this study, we compare the photometric properties of the Chang’e-5 landing site to those of the Reiner Gamma swirl. Because the physical effects of a landing rocket jet on the lunar regolith are relatively well known, these observations can provide important insights into the physical properties of lunar swirls.Methods.We determined the single scattering albedo, opposition effect strength, and surface roughness of the Reiner Gamma swirl and the Chang’e-5 landing site with their respective statistical uncertainties based on the Hapke model and Bayesian inference sampling.Results.The Chang’e-5 landing site and the Reiner Gamma swirl exhibit similar photometric properties, in particular: an increased albedo and a reduced opposition effect strength. Additionally, the landing site is about 20% less rough compared to the surrounding area.Conclusions.These findings suggest that the swirl surface is less porous compared to the surrounding surface, similarly to a landing site where the top layer of the regolith has been blown away effectively so that the compactness was increased. We conclude that external mechanisms that are able to compress the uppermost regolith layer are involved in lunar swirl formation, such as interactions with the gaseous hull of a passing comet.
Ceramic-on-Ceramic Total Hip Arthroplasty for Avascular Necrosis: 13-Year Average Follow-Up of Patients Under 50 Years Old
Authors: Webb Matthew L. , Perez Brian A. , Koressel Joseph E. , Kerbel Yehuda E. , Scanlon Christopher M. , Evangelista Perry J. , Kamath Atul F. , Nelson Charles L. 
Published: May 2023

AbstractThe long-term survival of ceramic-on-ceramic bearings in young, active patients with osteonecrosis undergoing total hip arthroplasty (THA) is unknown. A previously published study demonstrated a high activity level in these young patients at 5-year follow-up, and this is a second follow-up report on this previously reported series. The purpose of this study is to determine whether high activity level is associated with ceramic-on-ceramic THA failure at long-term follow-up. This is a retrospective review of a single-surgeon consecutive series of index cases performed between 2003 and 2010. Inclusion criteria were ceramic-on-ceramic THA articulations in patients younger than 50 with a diagnosis of osteonecrosis. Mean follow-up was 12.5 years (range 9–17). Data were collected by survey via mail, telephone, e-mail, and social media. Preoperative and postoperative Western Ontario and McMaster University (WOMAC) Arthritis Index and University of California at Los Angeles (UCLA) activity scores were collected. Student t-tests were used as appropriate. There were 97 patients in this series. Mean age at THA was 36 (range 14–50). Since the prior report, four more patients have been confirmed deceased, and four more have been lost to follow-up. We confirmed that six patients were deceased and 42 were otherwise lost to follow-up. The response rate was 54%. The vast majority of patients were highly active at latest follow-up (73% with UCLA scores between 7 and 10). UCLA scores (1–10 scale) improved from a preoperative mean of 3.4 to postoperative 7.1 (p < 0.001). WOMAC scores (1–100 scale) increased from preoperative mean of 38.7 to postoperative 86.1 (p < 0.001). At latest follow-up, four patients had undergone revision surgery. There were no ceramic component failures. One patient underwent early revision for femoral component loosening, one was revised for chronic pain at another institution. Since the prior report there have been two additional revisions; one patient was revised for instability at 5-year postop, and one patient was revised for periprosthetic fracture at 10 years. Despite additional loss to follow-up and lack of clinical and radiographic measures, this survey study suggests that at long-term follow-up ceramic bearings accommodate high activity level with excellent component survivorship (93%) in young patients undergoing THA.
Application of Chitosan Biomaterials in Dentistry—A Narrative Review
Authors: Krishna Nidhish , Lolayekar Nikita 
Published: May 2023

AbstractChitosan is a versatile natural biomaterial that has been researched for a range of bio-dental applications. It possesses various desirable qualities such as biocompatibility, hydrophilicity, biodegradability, and a broad antibacterial range (covering Gram-negative and Gram-positive bacteria as well as fungi). Moreover, the molecular structure contains reactive functional groups, which provide numerous reaction sites and possibilities for the formation of electrochemical interactions at the cellular and molecular levels. Chitosan's unique features have attracted material scientists from all over the world to explore its applications in dentistry. The objective of this review is to highlight the creation of new chitosan biomaterials and as to how it is a vital component for the improvement and modification of existing dental materials being used.
Potential Combination of Acemannan Sponge and Calcium Phosphate Cement-Calcium Sulfate Hemihydrate (CPC-CSH) as Direct Pulp Capping Alternative Materials for Reparative Dentin Formation
Authors: Irmawati Anis , Pratiwi Annisa Indah , Azizi Muh Rizki Aqsanu , Apriliyanti Nurul Annisa , Annafi Ayu , Azzaim Yassir Ahmad , Alqhtani Ala'a Saif 
Published: May 2023

Abstract
Background Health problem that affects hard tissues of the teeth is dental caries, which is experienced by around 2.3 billion people in the world, with prevalence in Indonesia reaching 88.8. Direct pulp capping (DPC) is a vital pulp therapy used to maintain pulp vitality. Calcium hydroxide (Ca(OH)2) is the gold standard pulp capping material, but has poor adhesion to dentin and mechanical properties, bacterial infiltration, formation of tunnel defects in the pulp resorption, and dentin bridge. Therefore, it is necessary to develop alternative therapy, namely, a combination of acemannan sponge and calcium phosphate cement (CPC)-calcium sulfate hemihydrate (CSH) for reparative dentin formation.
Purpose This article describes the potential combination of acemannan sponge and CPC-CSH as DPC alternative materials for reparative dentin formation.
Reviews Acemannan extracted from aloe vera exhibits anti-inflammatory, antimicrobial, and cytocompatibility properties. As DPC material, acemannan induces pulp proliferation and differentiation to osteoblast-like, growth factor synthesis, and promotes reparative dentin formation. However, acemannan sponge is radiolucent, allowing misinterpretation between pulp and acemannan sponge. CPC can be combined with CSH to shorten the setting time. CPC-CSH is radiopaque, has good compressive strength, and biocompatibility. CPC-CSH is a calcium-based material with neutral pH that can induce the dentin bridge formation. CPC-CSH showed good bioactivity because it induces the formation of apatite which plays a significant part in dentin and pulp regeneration.
Conclusion The combination of acemannan sponge and CPC-CSH has the potential as DPC alternative materials for reparative dentin formation.
Changes in Thymic Size and Immunity Are Associated with Bronchopulmonary Dysplasia
Authors: Chen Yan , Song Yue , Peng Hua , Li Jing , Zhao Cong , Liu Ding , Tan Jing , Liu Yalan 
Published: May 2023

Objective Preterm infants with bronchopulmonary dysplasia (BPD) are at increased risk for dysfunctional immune responses in the postnatal period. This study aimed to verify the hypothesis that thymic function is altered in infants with BPD and changes in the expression of thymic function-related genes affect thymic development.
Study Design Included in the study were infants who had a gestational age ≤32 weeks and survived to a postmenstrual age of ≥36 weeks. The clinical features and thymic size were comparatively studied between infants with and without BPD. Thymic function and the expression of thymic function-related genes were determined in BPD infants at birth, week 2, and 4 of life. The thymic size was ultrasonographically assessed in terms of the thymic index (TI) and thymic weight index (TWI). T-cell receptor excision circles (TRECs) and gene expression were quantitatively determined by real-time quantitative reverse transcription polymerase chain reaction.
Results Compared to non-BPD infants, their BPD counterparts had a shorter GA, lower birth weight, lower Apgar scores at birth, and were more likely to be of the male gender. BPD infants had an elevated incidence of respiratory distress syndrome and sepsis. TI was 1.73 ± 0.68 versus 2.87 ± 0.70 cm3 and TWI was 1.38 ± 0.45 versus 1.72 ± 0.28 cm3/kg in the BPD group versus the non-BPD group (p < 0.05). In BPD infants, no significant changes were observed in thymic size, lymphocyte counts, and TREC copy numbers at the first 2 weeks (p > 0.05), but they all exhibited a significant increase at week 4 (p < 0.05). BPD infants presented a trend toward increased expression of transforming growth factor-β1 and decreased expression of forkhead box protein 3 (Foxp3) from birth to week 4 (p < 0.05). Nonetheless, no significant difference was found in IL-2 or IL-7 expression at all time points (p > 0.05).
Conclusion For preterm infants with BPD, reduced thymic size at birth might be associated with impaired thymic function. Thymic function was developmentally regulated in the BPD process.
Key Points
No evidence of superconductivity in a compressed sample prepared from lutetium foil and H2/N2 gas mixture
Authors: Cai Shu , Guo Jing , Shu Haiyun , Yang Liuxiang , Wang Pengyu , Zhou Yazhou , Zhao Jinyu , Han Jinyu , Wu Qi , Yang Wenge , Xiang Tao , Mao Ho-kwang , Sun Liling 
Published: May 2023

A material described as lutetium–hydrogen–nitrogen (Lu-H-N in short) was recently claimed to have “near-ambient superconductivity” [Dasenbrock-Gammon et al., Nature 615, 244–250 (2023)]. If this result could be reproduced by other teams, it would be a major scientific breakthrough. Here, we report our results of transport and structure measurements on a material prepared using the same method as reported by Dasenbrock-Gammon et al. Our x-ray diffraction measurements indicate that the obtained sample contains three substances: the face-centered-cubic (FCC)-1 phase (Fm-3m) with lattice parameter a = 5.03 Å, the FCC-2 phase (Fm-3m) with a lattice parameter a = 4.755 Å, and Lu metal. The two FCC phases are identical to the those reported in the so-called near-ambient superconductor. However, we find from our resistance measurements in the temperature range from 300 K down to 4 K and the pressure range 0.9–3.4 GPa and our magnetic susceptibility measurements in the pressure range 0.8–3.3 GPa and the temperature range down to 100 K that the samples show no evidence of superconductivity. We also use a laser heating technique to heat a sample to 1800 °C and find no superconductivity in the produced dark blue material below 6.5 GPa. In addition, both samples remain dark blue in color in the pressure range investigated.