bluuebunny/crossref_metadata_2025_split huggingface dataset:
ZnO-incorporated chitin hydrogels for infected wound therapy
Authors: Zhang Hongli , Xu Mengqing , Wang Liang , Luo Haihua , Wu Shuangquan , Gao Tian , Wu Qiong , Xu Huan 
Published: September 2023

Abstract
Chronic wounds caused by pathogenic bacterial infections have been a worldwide medical threat and challenge, ZnO is a promising antibacterial agent to promote infected wound healing. However, ZnO particles need to be with a wound dressing material to improve low-dose antibacterial efficacy while reducing cytotoxicity. The natural polysaccharide, chitin-based hydrogels can be applied as a preferential supporting matrix for the deposition of ZnO. In this study, we developed chitin/ZnO composite hydrogels (CZG hydrogels), which were applied for the biofilm-infected full-thickness wound treatment. The three-dimensional hydrophilic network structure of chitin hydrogels provided a large number of absorption sites for Zn2+ ions, and CZG hydrogels were prepared by in situ synthesis of ZnO. CZG hydrogels had potent broad-spectrum and long-lasting antibacterial activity, good bacteriostatic ability against high concentration of bacterial fluids. The in vivo studies showed that CZG hydrogels have a significant effect of accelerating biofilm-infected wound healing. Collectively, this work confirmed that chitin hydrogels could be applied as a preferential natural supporting matrix for the deposition of inorganic metal and metal oxide nanoparticles, and provided alternative antibacterial dressing for treating microbial infections and promoting wound healing.
A comprehensive study of noise pollution level in eco- friendly CNC wood machining
Authors: Rabiei Farshad , Souri Shiva 
Published: September 2023

Abstract
Although CNC (Computer Numerical Control) machinery have many technological and economic advantages in wood machining, they have serious health problems especially in case of noise pollution level (NPL). In this research, the effective parameters on NPL in CNC wood milling are investigated and optimized after modeling. For this purpose, the design of the experiment was done based on the RSM (Response Surface Method) and subsequently 27 experimental tests were performed. The results of the analysis of variance shows that the parameters of cutting speed, feed speed and depth of cut have a great impact on the noise level (respectively). In general, increasing depth of cut, feed rate and cutting speed leads to increasing NPL. Cutting speed has more effect than others. The step over does not have a significant effect on the NPL. By changing machining parameters amount of noise level changes from 97.4 to 103.8 dB (= decibel). Also in 100% of experimental tests noise level is higher than NIOSH standard value (85 dB) (NIOSH = The National Institute for Occupational Safety and Health). Therefore, it is necessary for all CNC wood workers to be entered in a hearing loss prevention program (HLPP). In the next step, based on the RSM, a quadratic regression equation was derived and optimized by genetic algorithm (GA) after validation. By choosing the optimum parameters depth of cut = 3.2mm, feed rate = 58mm/s, cutting speed = 12730 rpm and step over = 6.4 mm the level of noise pollution is reduced to 96.2 dB. This prediction tested and validated by experimental test (95.8 dB).
Cuk Converter based Wireless Power Transfer System for Low Power Applications: A Grey Wolf Optimization Approach
Authors: Amin Ifte Khairul , Jaman Azam , Islam Md. Nahid 
Published: September 2023

Abstract
Wireless Power Transfer (WPT) has emerged as a compelling technology offering a wide range of applications. Even though it is predominantly used in high-power applications such as Electric Vehicle (EV) charging, nowadays it is opening up a realm of possibilities for low-power devices. The ability to transmit power wirelessly revolutionizes seamless integration and enhanced functionality to power electronic devices such as smartphones, wearables, Internet-of-Things (IoT) sensors, medical implants and smart-home devices. This paper presents a novel strategy for cuk converter based WPT system designed in MATLAB/Simulink environment specifically for low power applications. The control system for the proposed technique has been tuned using Grey-wolf optimization (GWO) owing to its efficient and powerful search capabilities. The simulation results with integration of the cuk converter exhibit robustness against variations in load conditions, improve power conversion efficiency, and overall system performance, making it a promising solution for low voltage wireless power transfer applications.
A Comprehensive Pan-Cancer Analysis of 33 Human Cancers Reveals the Immunotherapeutic Value of Opa interacting protein 5
Authors: Dong Wenjing , Luo Jinquan , Yuan Runqiang , Li Yueming , Feng Shengxing , Zhou Dongsheng , Wang Jiaxin , Huang Jian , Gong Mancheng 
Published: September 2023

Abstract
Background: In previous research, the potential significance of the interaction between Opa interacting protein 5 (OIP5) and tumor immunotherapy has been documented. Nevertheless, a comprehensive understanding of the therapeutic value of this interaction remains lacking. Therefore, the objective of our study was to investigate the correlation between OIP5 and cancer immunotherapy in a total of 33 human malignancies
Methods: The data on the clinical characteristics and gene expression of 33 types of human cancers were extracted from The Cancer Genome Atlas database. The immunotherapy groups, which consist of GSE67501, GSE78220, and IMvigor210, were obtained from the Gene Expression Omnibus database. We also evaluated the prognostic significance of OIP5 by analyzing the clinical parameters related to tumor stage. Through single-sample gene set enrichment analysis, we examined the activity of OIP5 to illustrate the disparity between the protein expression level and transcriptome. In order to enhance our comprehension of the role played by OIP5 in the immunotherapy of human cancers, we investigated its association with the tumor microenvironment and its correlation with immune processes/elements such as the infiltration of immune cells, immune stimulants, immune inhibitors, and the major histocompatibility complex. We also conducted research to explore the relevant pathways that are linked to the signaling of OIP5 in cancer. Additionally, we explored the correlation between OIP5 and two biomarkers of immunotherapy, namely tumor mutational burden and microsatellite instability. Lastly, we examined the relationship between OIP5 and the response to immunotherapy using the three independent immunotherapy groups mentioned earlier.
Results: OIP5 was significant associated with tumor stage (9/21) in the studied human cancers and revealed potential prognostic value for predicting patient survival. Consistency has been observed between OIP5 activity in some cancers (21/33). Generally, OIP5 displayed a strong correlation with immune cell infiltration, immune modulators, and immunotherapeutic markers. Moreover, overexpression of OIP5 was markedly related to immune-relevant pathways. What’s more, significantly positive correlation was observed between OIP5 and the immunotherapeutic response in cohorts of GSE78220.
Conclusions: This study provided evidence regarding the function of OIP5 and its role in clinical treatment by investigating the immunotherapeutic value of OIP5 in 33 human cancers. However, the current results are preliminary and require further verification for the reason of adopting a bioinformatics approach.
In-house real-time PCR to detection Toxoplasma gondii in pregnant women candidates for amniocentesis and comparison with nested PCR and serologic methods in several clinical samples
Authors: Abedian Rohallah , Fakhar Mahdi , Kordi Shirafkan , Roshan Hadi Shokrollahnia , Rahmani Zahra , Esboei Bahman Rahimi , Montazeri Mahbobeh , Hezarjaribi Hajar Ziaei 
Published: September 2023

Abstract
Background: Toxoplasmosis is a common parasitic infection caused by Toxoplasma gondii (T. gondii), an obligate and intracellular parasite, that affects both humans and animals globally. It can cause severe damage to neonates during pregnancy and immunocompromised patients. Therefore, finding a new method with high sensitivity for the diagnosis of toxoplasmosis that involves low-risk sampling is crucial for patient management. This study aims to diagnose toxoplasmosis in pregnant women from plasma, amniotic fluid, buffy coat, and urine specimens using serological, nested PCR, and real-time PCR tests.
Methods: We collected amniotic fluid, blood, and urine samples from 100 pregnant women referred to a gynecologist in Mazandaran province, northern Iran, who were candidates for amniocentesis. We performed anti-T. gondii IgM, IgG, and IgG avidity tests using enzyme-linked immunosorbent assay (chemiluminescence, ELISA), as well as nested PCR, real-time PCR, and multilocus PCR genotyping using RE and GRA6 target genes, respectively.
Results: Based on the serologic test, 52% of subjects were seropositive (51 samples were positive for IgG, and 1 was positive for both IgG and IgM antibodies), and all cases showed high avidity. Among 100 amniotic fluid samples, 2 and 3 cases tested positive using nested and real-time PCR, respectively, while all samples from buffy coat and urine were negative. All positive DNA samples were genotyped as type I.
Conclusions Our study results suggest that diagnosing congenital toxoplasmosis is achievable using a combination of serological and molecular tests. We also found that the real-time PCR method is more sensitive than the nested PCR.
The effect of multimedia-based peer-to-peer education on anxiety among Covid-19 patients: A parallel randomized clinical trial
Authors: Shariati Esmail , Bakhshiarab Amirheidar , Shariati Zahra , Hosseinzadeh Ali , Bagheri Hossein 
Published: September 2023

Abstract
Background
The COVID-19 pandemic has been associated with increased patient anxiety, necessitating the use of educational communication programs considering the capacity of medical centers to reduce the psychological burden of Covid-19 patients. This research aimed to determine the effect of multimedia-based peer-to-peer education on anxiety among Covid-19 patients.
Methods
In this clinical trial, 100 COVID-19 patients hospitalized from May to August 2021 were included. Participants were assigned into two groups; i.e., control (routine ward interventions) and experimental (multimedia-based peer education) via quadruple blocking. The intervention content included a 20-minute short educational video, which involved interviews with two COVID-19 patients who were discharged from the hospital. One of these patients was admitted to the general ward and the other to the COVID-19 ICU ward. The demographic questionnaire was completed before the intervention, and the Zung Self-Rating Anxiety Scale (SAS) was also completed pre- intervention and post-intervention by participants in both groups. Data analysis was carried out using descriptive statistics and analytical statistics tests in SPSS ver. 24.
Results
There was no significant difference between the two groups in terms of demographic variables (P > 0.05). There was also no significant difference between the two groups regarding mean and standard deviation of anxiety scores pre-intervention (P = 0.327). However, the mean post-intervention anxiety score in the experimental group was significantly lower than in the control group (P < 0.001).
Conclusion
Considering limitations associated with the implementation of educational-communication programs in COVID-19 wards, it is suggested to use multimedia-based peer education as a highly practical solution for this group of patients.
Trial registration:
registered with Iranian Registry of Clinical Trials (IRCT) dated on 11/01/2021, registration number IRCT20200223046586N3.
Involvement of IL17A and IL17RA variants in interleukin-17A levels and disease activity in ulcerative colitis
Authors: Zanotti Mariana Paula Sanchez , Alcântara Camila Cataldi , Inoue Cláudia Junko , Gonçalves Beatriz Piantoni , Espinosa Beatriz Rabello , Cassela Pedro Luiz Cândido Souza , Trigo Guilherme Lerner , Ahrens Tainah Mendes , Batisti Marcell Alysson , Reiche Edna Maria Vissoci , Simão Andrea Name Colado 
Published: September 2023

Abstract
Ulcerative colitis (UC) is characterized by chronic inflammation of the large intestine. Disease activity and mucosal injury are affected by the Th17 cells and their interleukin (IL)-17A. A link between genetic variations in IL17A genes and autoimmunity susceptibility has been reported; however, the results in UC are limited and contradictory. The aim of this study was to evaluate the involvement of IL17A and IL17A receptor (IL17RA) variants on susceptibility, IL-17A plasma levels, and endoscopic activity in UC. The case-control study included 104 UC patients and 213 healthy controls. Patients were divided according to the endoscopic activity: remission/mild (Mayo ≤ 1) and moderate/severe (Mayo > 1). The IL17A (rs3819024, rs3819025) and IL17RA (rs2241043, rs2241049, rs6518661) variants were genotyped by allelic discrimination using the real time polymerase chain reaction. IL-17A plasma levels were determined using microspheres immunofluorimetric assay. Neither IL17A nor IL17RA variants were associated with UC susceptibility. However, IL17A GA genotype (rs3819024) was associated to elevated levels of IL-17 only in UC patients; moreover, patients with the G allele (dominant model) of IL17RA (rs2241049) showed 2.944 more chance of developing moderate/severe disease. In conclusion, IL17A GA genotype (rs3819024) was associated with elevated IL-17A plasma levels in UC patients but not in controls. In addition, AG + GG genotypes of IL17RA (rs2241049) variant were associated to moderate/severe UC. The results suggest a possible hidden interaction between the IL17A rs3819024 variant and other genetic, epigenetic or environmental variables in the IL-17A expression that is present in patients with UC and absent in controls.
Metadichol induced differentiation of pancreatic ductal cells (PANC-1) into insulin-producing cells
Authors: Raghavan Palayakotai R 
Published: September 2023

Abstract

Endocrine gene expression in PANC-1, a type of pancreatic cancer cell, has been studied in the context of their potential to be reprogrammed toward a normal, differentiated state. Alkaline phosphatase activity has also been shown in pluripotent stem cells to differentiate between feeder and parental cells in reprogramming experiments. Metadichol®-based cell programming holds promise as a versatile and potentially safer approach for manipulating cellular behavior without the use of viral vectors, gating, or CRISPR. This study investigated the multifold increase in the gene expression of CA9, GCG, INS MAFA, NEUROD1, NGN3, NKX2-2, PAX6: PDX1, SLC2A2, FOXO1, and SIRT1 using qRT‒PCR. These genes regulate endocrine cell development in the pancreas and are involved in insulin and glucagon secretion. Gene network analysis is presented to show how Metadichol-induced expression leads to a closed loop feedback network and biological process that would help in mitigating diabetes and other related disorders.
Evaluating the impact of leakage in intermittent water supply networks considering justice index: A case study
Authors: Dadras Mohaddeseh , Masoumi Fariborz , Masoumzadeh Sina , Najjar-Ghabel Saeid , Nikoo Mohammad Reza , Yazdani Shakiba 
Published: September 2023

Abstract

Intermittent water supply (IWS) is one of the effective methods to manage the consumption of urban water networks under water scarcity conditions. However, it is essential to minimize unfair water distribution in this method by defining a proper strategy. This study utilized the EPANET pressure-dependent hydraulic analysis and the gray wolf optimization algorithm to achieve maximum volumetric reliability under different scenarios in a district of the Hamedan urban water distribution network in Iran. The volumetric reliability of the network was evaluated in the IWS condition regardless of justice constraints, with the justice constraint, and by considering the leakage in the IWS network with the justice constraint. The first scenario demonstrated that the reliability decreased by an average of 4.6% for every meter of water level reduction in the tank. The second scenario revealed that the objective function was negligibly affected by the variation of the justice constraint; however, fluctuation of the water level in the tank significantly affected the volumetric reliability. In the third scenario, the objective function value was significantly impacted by leakage, ranging from 0 to 0.3 (representing the absence and presence of leakage in 30% of the nodes, respectively), resulting in an average decrease of about 17%.
Weather system classification and water vapor transport characteristics of extreme value rainstorm in northwestern Hunan
Authors: Zhao Enrong , Yao Qian , Pan Xiaolong , Yao Rong , Chen Hongzhuan , Su Tao 
Published: September 2023

Abstract
Using meteorological analysis, composite analysis and water vapor trajectory analysis, the extreme value rainstorm process in northwest Hunan was analyzed. The results show that three types are summarized: the Southwest Vortex and Warm Shear Line Pattern (SVWSLP), the Subtropical High Edge Pattern (SHEP) and the Cold Trough and Shear Line Pattern (CTSLP). The main influence systems are upper trough, southwest vortex, shear line, low-level jet and subtropical high edge. For SVWSLP, the water vapor transport channels are only from the low-latitude ocean whether it is affected by long-distance typhoons. For SHEP, the main water vapor channel comes from the long-distance ocean and is finally transported to northwestern Hunan around 650 hPa in the form of warm and wet airflow, whether it is affected by long-distance typhoons. The CTSLP appears a significant water vapor confrontation between the north and the south and the baroclinicity of the atmosphere in the rainstorm area. The southern and western boundaries are the input boundary, while the eastern and northern boundaries are the outflow boundary. Therefore, one of the three types of weather systems appears in northwestern Hunan in May–August, with strong water vapor transport from the ocean surface, which is likely to cause extreme rainstorm.
Alkaloids as Additional Weapons in the Fight against Breast Cancer:
A Review
Authors: Chahat  , Jha Keshav Taruneshwar , Bhatia Rohit , Chawla Pooja A. 
Published: September 2023

:
Breast carcinoma is among the most frequent cancerous tumour in females
around the globe. The major modalities now employed in the therapeutic management of
breast cancer include surgeries, chemotherapy, and specialized medicines. Despite their
potential to help individuals' problems, they are also associated with many negative impacts.
As a result, natural products are increasingly regarded to be a preferable alternative.
Alkaloids are essential biochemical substances that can be used to develop new drugs.
Numerous alkaloids that originate from natural plants have been shown in vitro and in vivo
to have anti-proliferation and anti-metastasis actions on different kinds of carcinoma.
According to the data collected in this study, the utilization of alkaloids as anti-tumor
medicines appears to be extremely potent; nevertheless, extensive studies and clinical trials
are required before utilizing individual alkaloids. In this overview, we provide a detailed and
vital exploration of pre-existing alkaloids possessing anti-tumor activities due to bioactive
compounds. This study also includes an overview of synthesized analogues and pharmacological
characteristics that will be beneficial to scientists working on alkaloids for medicinal
purposes. In a recent survey of the literature, alkaloids are an important component of plantderived
antitumor medicines that hold great potential for the future development of cancer
therapy and preventive therapies. We have also discussed structural analysis relationship
(SAR) studies. Moreover, it covers clinical trial medications and FDA-approved medicines
from the last five years that will be useful in further research.
Neuroprotective Effect of Natural Indole and β-carboline Alkaloids against Parkinson’s Disease: An Overview
Authors: Shome Abhimannu , Chahat  , Chawla Viney , Chawla Pooja A. 
Published: September 2023

:
Parkinson’s disease (PD) is a devastating neurodegenerative condition that
mostly damages dopaminergic neurons in the substantia nigra and impairs human motor
function. Males are more likely than females to have PD. There are two main pathways
associated with PD: one involves the misfolding of α-synuclein, which causes neurodegeneration,
and the other is the catalytic oxidation of dopamine via MAO-B, which
produces hydrogen peroxide that can cause mitochondrial damage. Parkin (PRKN), α-
synuclein (SNCA), heat shock protein (HSP), and leucine-rich repeat kinase-2 (LRRK2)
are some of the target areas for genetic alterations that cause neurodegeneration in Parkinson's
disease (PD). Under the impact of 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine
(MPTP), which is also important in Parkinson's disease (PD), inhibition of mitochondrial
complex 1 results in enhanced ROS generation in neuronal cells. Natural products are still
a superior option in the age of synthetic pharmaceuticals because of their lower toxicity
and moderate side effects. A promising treatment for PD has been discovered using betacarboline
(also known as "β-carboline") and indole alkaloids. However, there are not
many studies done on this particular topic. In the herbs containing β-carbolines and indoles,
the secondary metabolites and alkaloids, β-carbolines and indoles, have shown neuroprotective
and cognitive-enhancing properties.

:
In this review, we have presented results from 18 years of research on the effects of indole and
β-carboline alkaloids against oxidative stress and MAO inhibition, two key targets in PD. In
the SAR analysis, the activity has been correlated with their unique structural characteristics.
This study will undoubtedly aid researchers in looking for new PD treatment options.