bluuebunny/crossref_metadata_2025_split huggingface dataset:
Circ_0003340 regulates the expression of ENAH to affect the development of esophageal cancer through miR‐874‐3p
Authors: Wang Jingyi , Zhao Ning , Peng Shengzu , Zhang Tao 
Published: February 2023

AbstractBackgroundEsophageal cancer is a malignant tumor with a poor prognosis and high incidence. Circular RNAs (circRNAs) have been shown to be involved in the pathogenesis of cancers, including esophageal cancer. Here, we explored the precise role of circ_0003340 in esophageal cancer development.MethodsThe expression levels of circ_0003340, miR‐874‐3p and enabled homolog (ENAH) were detected by quantitative real‐time polymerase chain reaction and western blot. Subcellular localization and RNase R assays were used to characterize circ_0003340. Cell Counting Kit 8, flow cytometry, transwell assays were used to analyze cell proliferation, apoptosis, migration and invasion. The effect of circ_0003340 on tumor growth was assessed by tumor experiments in vivo. Dual‐luciferase reporter assay was used to analyze the relationship between miR‐874‐3p and circ_0003340 or ENAH.ResultsCirc_0003340 was mainly located in the cytoplasm and was upregulated in esophageal cancer tissues and cells. Circ_0003340 knockdown inhibited cell proliferation, migration, invasion, glucose consumption, and lactate production and induced cell apoptosis in esophageal cancer cells. Moreover, circ_0003340 knockdown suppressed tumor growth in vivo. MiR‐874‐3p was reduced in esophageal cancer tissues and cells, and it was a molecular mediator of circ_0003340 function in esophageal cancer cells. ENAH was identified as a direct and functional target of miR‐874‐3p in esophageal cancer cells. The promotion effect of circ_0003340 on ENAH was ameliorated by miR‐874‐3p.ConclusionThe data demonstrated that circ_0003340 promoted the progression of esophageal cancer through miR‐874‐3p/ENAH axis, which might provide novel therapeutic targets for esophageal cancer intervention.
The impact of COVID‐19 on trends in alcohol use disorder treatment in Veterans Health Administration
Authors: Perumalswami Ponni V. , Kilpatrick Sidonie , Frost Madeline C. , Adams Megan A. , Kim Hyungjin Myra , Zhang Lan , Lin Lewei 
Published: February 2023

AbstractBackground and AimsThe COVID‐19 pandemic disrupted health‐care provision in the United States and prompted increases in telehealth‐delivery of care. This study measured alcohol use disorder (AUD) treatment trends across visit modalities before and during COVID‐19.Design, Setting, Participants and MeasurementsWe conducted a national, retrospective cohort study with interrupted time‐series models to estimate the impact of COVID‐19 on AUD treatment in the Veterans Health Administration (VHA) in the United States during pre‐COVID‐19 (March 2019 to February 2020) and COVID‐19 (March 2020 to February 2021) periods. We analyzed monthly trends in telephone, video and in‐person visits for AUD treatment and compared patient and treatment characteristics of patients receiving AUD treatment between the pre‐COVID‐19 and COVID‐19 periods. AUD was defined using International Classification of Diseases, 10th revision (ICD‐10) codes for alcohol abuse (F10.1) and alcohol dependence (F10.2), which have previously been used to study AUD in VHA.FindingsThe predicted percentage of VHA patients with an AUD diagnosis receiving any AUD treatment at the beginning of the pre‐COVID period was 13.8% (n = 49 494). The predicted percentage decreased by 4.3% (P = 0.001) immediately at the start of the COVID‐19 period due to a decline in AUD psychotherapy. Despite an increase of 0.3% per month (P = 0.026) following the start of COVID‐19, the predicted percentage of VHA patients with an AUD diagnosis receiving any AUD treatment at the end of the study period remained below the pre‐COVID‐19 period. In February 2021, AUD psychotherapy visits were primarily delivered by video (50%, 58 748), followed by in‐person (36.6%, 43 251) and telephone (13.8%, 16 299), while AUD pharmacotherapy visits were delivered by telephone (38.9%, 3623) followed by in‐person (34.3%, 3193) and video (26.8%, 2498) modalities. Characteristics of VHA patients receiving AUD treatment were largely similar between pre‐COVID‐19 and COVID‐19 periods.ConclusionsDespite increased telehealth use, the percentage of United States Veterans Health Administration patients with an alcohol use disorder (AUD) diagnosis receiving AUD treatment declined during COVID‐19 (March 2020 to February 2021) mainly due to a decrease in psychotherapy.
Genomic regions underlying positive selection in local, Alpine cattle breeds
Authors: Signer‐Hasler Heidi , Casanova Lucas , Barenco Alex , Maitre Blaise , Bagnato Alessandro , Vevey Mario , Berger Beate , Simčič Mojca , Boichon Denis , Capitan Aurélien , Medugorac Ivica , Bennewitz Jörn , Mészáros Gábor , Sölkner Johann , Drögemüller Cord , Flury Christine 
Published: February 2023

AbstractWe used genome‐wide SNP data from 18 local cattle breeds from six countries of the Alpine region to characterize population structure and identify genomic regions underlying positive selection. The geographically close breeds Evolèner, Eringer, Valdostana Pezzata Nera, and Valdostana Castana were found to differ from all other Alpine breeds. In addition, three breeds, Simmental, and Original Braunvieh from Switzerland and Pinzgauer from Austria built three separate clusters. Of the 18 breeds studied, the intra‐alpine Swiss breed Evolèner had the highest average inbreeding based on runs of homozygosity (FROH) and the highest average genomic relationship within the breed. In contrast, Slovenian Cika cattle had the lowest average genomic inbreeding and the lowest average genomic relationship within the breed. We found selection signatures on chromosome 6 near known genes such as KIT and LCORL explaining variation in coat color and body size in cattle. The most prominent selection signatures were similar regardless of marker density and the breeds in the data set. In addition, using available high‐density SNP data from 14 of the breeds we identified 47 genome regions as ROH islands. The proportion of homozygous animals was higher in all studied animals of local breeds than in Holstein and Brown Swiss cattle, the two most important commercial breeds in the Alpine region. We report ROH islands near genes related to thermoregulation, coat color, production, and stature. The results of this study serve as a basis for the search for causal variants underlying adaptation to the alpine environment and other specific characteristics selected during the evolution of local Alpine cattle breeds.
Examining the abundance and habitat use of golden‐rumped sengi (Rhynchocyon chrysopygus) in Arabuko‐Sokoke Forest in Kenya
Authors: Ondoro Rael Nelly Nyanchama , Okeyo Benards , Jackson Colin 
Published: February 2023

AbstractThe International Union for Conservation of Nature (IUCN) classifies the golden‐rumped sengi (Rhynchocyon chrysopygus) as an ‘endangered’ species’. They are endemic to fragmented forests in eastern Kenya. Existing evidence indicates a decline in their population in Arabuko‐Sokoke Forest; however, these studies were conducted over a decade ago. This study assessed their abundance in three vegetation types. This study was conducted in the months of September, October and November in the year 2019. We used line transects in the forest types to locate nests and recorded the distances to the nests together with habitat variable measurements. We used (10 by 10 m) quadrats to obtain data on deadwood. We used line transect distance sampling to estimate sengi density and abundance. We employed linear regression models to examine the association between the number of nests per transect and deadwood volume, while means and proportions were used to examine the determinants of sengi nesting sites. The golden‐rumped sengi population was estimated to be 19,423. Besides, deadwood volume was not associated with nest site occurrence. Although findings from this study indicate an increase in the golden‐rumped sengi population from 12,750 individuals in 2009, continued monitoring is essential to inform conservation measures.
Unmanned aerial vehicle outputs and associated field measurements of the herbaceous and tree layers of the Senegalese savannah
Authors: Taugourdeau Simon , Cofélas Fassinou , Bossoukpe Marina , Diatta Ousmane , Ndiaye Ousmane , Diehdiou Antoine , N'Goran Ange , Audebert Alain , Faye Emile 
Published: February 2023

AbstractUnmanned aerial vehicles (UAVs) are widely used to assess the vegetation of an ecosystem. This report presents three data sets from UAVs used on a savannah‐type ecosystem in Senegal. Two data sets contain an orthomosaic and a surface and terrain digital model (UAV outputs), along with associated field measurements on the tree and herbaceous layers, while the third data set contains an orthomosaic and digital surface model, along with field measurements on the herbaceous layers. One data set was compiled over the entire rainy season on the same plot (plot dataset). One was compiled across a landscape at the 7000 ha Dahra Djoloff research center (CRZ‐dahra Centre de recherche zootechnique de Dahra). The third data set was compiled across Senegal from the North to the Southeast of the country with wide variability in climate and soil conditions. All the data sets are in Open Access in the Zenodo repository. These data sets could be used in many different studies: calibration between field measurements and UAV outputs on a large scale, or used as an intermediate step between field and satellite images.
Trends in the epidemiology of fear of childbirth and association with intended mode of delivery: A nationwide register‐based cohort study in Finland
Authors: Vaajala Matias , Liukkonen Rasmus , Kuitunen Ilari , Ponkilainen Ville , Mattila Ville M. , Kekki Maiju 
Published: February 2023

AbstractIntroductionFear of childbirth (FOC) is a common obstetrical challenge that affects the health of women. The epidemiology of FOC has not been studied in Finland for the last decade. The aim of this study was to analyze the epidemiology and risk factors for FOC and to evaluate the association between FOC and the rate of elective cesarean section (CS) as an intended mode of delivery.Material and methodsData from the National Medical Birth Register were used to evaluate the epidemiology of FOC and to determine the main risk factors for FOC in Finland between 2004 and 2018. Nulliparous and multiparous women were analyzed separately. Logistic regression model was used to determine the main risk factors for FOC. Multivariable logistic regression model was used to assess the intended mode of delivery in those pregnancies with diagnosed maternal FOC. Adjusted odds ratios (aOR) with 95% confidence intervals (CIs) were calculated.ResultsA total of 1 million pregnancies were included. The annual rate of pregnancies with maternal FOC increased from 1.5% (CI: 1.4–1.6) in 2004 to 9.1% (CI: 8.7–9.3) in 2018 for all women. For nulliparous women, the rate increased from 1.1% (CI: 1.0–1.3) in 2004 to 7.1% (CI: 6.7–7.5) in 2018, and from 1.8% (CI: 1.7–2.0) in 2004 to 10.3% (10.0–10.7) in 2018 for multiparous women. The strongest risk factors for maternal FOC were higher maternal age and gestational diabetes. For multiparous women, the strongest risk factors were gestational diabetes and previous CS. The total odds for elective CS were notably higher among women with FOC (aOR 8.63, CI: 8.39–8.88).ConclusionsThe incidence of maternal FOC rose six‐fold during our study period. However, the numbers of elective CS among women with this diagnosis, which had earlier risen in parallel, leveled off in 2014.
Study on the strontium isotope standard in pig teeth excavated from Zaoshugounao site, Shaanxi Province of China
Authors: Liu Keyu , Ling Xue , Li Yue , Dou Haifeng , Qian Yaopeng , Wang Zhen 
Published: February 2023

AbstractIn archaeology, strontium isotope analysis is developing into an efficient scientific technique for tracing the movement of prehistoric humans and animals. Determining the local bioavailable 87Sr/86Sr ratio range is the key to distinguishing whether the human or animal is indigenous to the local area. It has been shown that the 87Sr/86Sr ratio in the enamel of pigs can be an excellent sample to determine the range of local strontium isotope ratio at the site. However, pigs may not be all local at the site, and there is no special study on whether pigs’ different ages and tooth types impact the local strontium isotope characteristics. In this paper, the tooth enamel of 19 pigs from the Zaoshugounao site for strontium isotope ratio (87Sr/86Sr) by multicollector–inductively coupled plasma mass spectrometry. There were no significant differences in 87Sr/86Sr ratios of local pigs at different months and different molar types, which did not affect the determination of the local strontium isotope standard. Based on the local strontium isotope ratio range (0.711056–0.711476), we found that five pigs were non‐local. The Jing and Wei river basins (in Guanzhong basin) and northern Shaanxi are likely sources of non‐pigs.
The veritable horns of a dilemma: Assessing homology of the parietal and squamosal asperities of Phrynosoma (Squamata: Iguania: Phrynosomatidae)
Authors: Powell G. Lawrence , Russell Anthony P. 
Published: February 2023

AbstractMembers of the genus Phrynosoma all exhibit asperities upon the parietal and squamosal. We examined μ‐CT scans of the dermatocrania of ontogenetic series of all but two of the species of Phrynosoma, and defined parietal and squamosal “horns”, distinguishing them from tubercles. We counted parietal and squamosal horns and used phylogenetic analysis to reconstruct the plesiomorphic horn numbers for these bones. Based upon this, we propose and test hypotheses of evolutionary homology for these asperities and conclude the following: (1) a plesiomorphic horn array is retained among the species of Phrynosoma; the horns are paramorphs, each identifiable as an evolutionary homologue; (2) an autapomorphic additional pair of parietal horns in P. solare has been recruited from a pair of tubercles associated with the plesiomorphic parietal horns; (3) an additional anterior squamosal horn in P. solare and the Brevicauda species is derived from a tubercle recruited homoplasiously in these taxa; (4) the anteriormost squamosal horn of the plesiomorphic series in the species of the coronatum species group is highly reduced and is subsumed within the posteriormost jugal asperity and (5) an apparent additional squamosal horn exhibited by P. ditmarsi and P. modestum is instead an enlarged submarginal tubercle, a homoplasy for these species.
Designing complex interventions: A description of the development of an intervention to reduce inequalities in planned dental visiting
Authors: Harris Rebecca Vera , Lowers Victoria , Van Der Zande Marieke , Stanley Margaret , Cooke Richard 
Published: February 2023

AbstractThere are multifaceted reasons for a social gradient in planned dental visiting involving various psycho‐social variables that interact with each other and the environment. Interventions in this area are therefore inevitably complex interventions. While guidance recommends undertaking theory and modelling work before experimental work is done, there is a shortage of descriptions of how this is done, especially in the field of oral health.ObjectivesTo describe theory, qualitative and public engagement work, and identification of behaviour change techniques (BCTs) to define features of an opportunistic dental visiting intervention for adult users of urgent dental care services.MethodsA systematic review and synthesis of theory, qualitative and quantitative work, along with expert input, generated a list of psycho‐social determinants linked to planned dental visiting intentions. Modelling involved ethnographic work in urgent dental care settings and work with members of the community from the targeted demographic. This enabled verification, in the context of their idiosyncratic expression for the target population in question, of behavioural determinants (BDs) identified in the theory phase. It also facilitated generating intervention material which was infused with the identity of the end user. BDs identified were then mapped to BCTs using an accepted BCT taxonomy and an intervention prototype developed. The prototype then underwent iterative testing with target users before it was ready for a feasibility trial.ResultsTheory and modelling identified five key intervention focuses: affordable resources (time/ cost), the importance of oral health, trust in dentists, embarrassment of having poor oral health and dental anxiety. Short videos were developed to incorporate role modelling which were well received. Prototype testing resulted in shifting from ‘if‐then’ plans to action planning.ConclusionsComplex intervention development involves an iterative rather than sequential process of combining theory, empirical work and user involvement, of which the article provides an example.
‘I wish that COVID would disappear, and we'd all be together’: Maintaining Children's friendships during the Covid‐19 pandemic
Authors: Carter Caron , Barley Ruth , Omar Arwa 
Published: February 2023

AbstractFriendship is a central focus in children's lives and is important for healthy development. During the Covid‐19 pandemic, children experienced restrictions on their interactions with friends. This research heard the voices of 10 children (7–11 years) in England regarding their friendships, drawing on data collected through creative participatory methods including drawings, photography and collages, and accompanying unstructured interviews. Findings provide new insights into how children endeavoured to maintain their friendships through virtual interactions, street/doorstep visits, and artwork, and how friendship disruption affected their well‐being. This paper argues for educators to heed the implications for the period of ‘Covid recovery’.
Homologues and homology and their related terms in phylogenetic systematics
Authors: Göpel Torben , Richter Stefan 
Published: February 2023

AbstractIn the field of phylogenetic systematics, the terms homology and homologue and their relationship to cladistic terms such as character, character state, synapomorphy and symplesiomorphy, as well as their relationships to each other, have been and are still discussed frequently. A recent re‐emergence of concepts of homology/homologue free of any reference to explanatory hypotheses prompted us to explore these concepts and their relationships to each other as well as to the concept of morpheme, as introduced recently. All concepts are examined with regard to their ontological status and their bearing in the epistemological process in morphology and phylogenetic systematics. To us, morphemes, homologues andin partemcharacter states refer to things (concrete objects in the ontological sense). However, although morphemes are exclusively descriptive, the latter two represent objects of causal explanations. Homologue always refers to the things themselves, yet a character state also can be a property or the absence of a thing. In this context, a character as a transformation series of character states does not represent a thing but a natural kind. Character states of one character are connected by homology relationships, i.e. common descent. Synapomorphy and symplesiomorphy represent different states of a single transformation series. A nonexplanatory, purely descriptive, concept of homologues is contradictory to its original as well as the post‐Darwinian, evolutionary, concept which refers to causal relationships between parts of organisms and their correspondences in the archetype or ancestor, respectively. Character states, homologues and synapomorphies/symplesiomorphies can only be approximated in the form of hypotheses. We argue that the high value of the concept of homology and its related concepts for evolutionary biology should be maintained by acknowledging their explanatory nature and that dilution with nonexplanatory (even idealistic) definitions should be avoided.
Influence of soft tissue thickness on marginal bone level around dental implants: A systematic review with meta‐analysis and trial‐sequential analysis
Authors: Bressan Eriberto , Guazzo Riccardo , Tomasi Cristiano , Peña Teresa Gutiérrez , Galindo‐Moreno Pablo , Caponio Vito Carlo Alberto , Sbricoli Luca , Canullo Luigi 
Published: February 2023

AbstractObjectivesThe aim of the present review and meta‐analysis was to evaluate the influence of soft tissue thickness on initial bone remodeling after implant installation.Materials and MethodsA literature search was conducted by two independent reviewers on electronic databases up to May 2022. Randomized controlled trials (RCTs) and controlled clinical trials (CCTs) performed on human subjects were included. The risk of bias was evaluated using Cochrane Collaboration's tool. Meta‐analysis and Trial Sequential Analysis (TSA) were performed on the selected articles. The primary outcome was marginal bone loss.ResultsAfter screening, 6 studies were included in the final analysis, with a total of 354 implants, and a follow‐up from 10 to 14 months. 194 implants were placed in a ≥ 2 mm soft tissue thickness, while 160 had <2 mm soft tissue thickness before implant placement. The included studies had a high level of heterogeneity (I2 > 50%). The meta‐analysis indicated a statistically significant difference between the two groups (0.54; p = .027) and the TSA analysis confirmed the results, despite the limited number of dental implants. Additional analysis showed that age and follow‐up parameters were not statistically significant factors influencing the bone loss (p = .22 and p = .16, respectively).ConclusionsBased on the available RCTS and CCTs, initial soft tissue thickness seems to influence marginal bone loss after a short follow‐up period. Based on TSA analysis, further studies are needed to assess the influence of the soft tissue thickness on marginal bone loss.PROSPERO registration number: CRD42021235324.
Modelling potential biotope composition on a regional scale revealed that climate variables are stronger drivers than soil variables
Authors: Rubanschi Sven , Meyer Sebastian T. , Hof Christian , Weisser Wolfgang W. 
Published: February 2023

AbstractAimEnvironmental conditions define the suitability of an area for biotopes, and any area can be suitable for several biotopes. However, most previous studies modelled the distribution of single biotopes ignoring the potential co‐occurrence of biotopes in one area, which limits the usefulness of such models for conservation and restoration planning. In this study, we described the potential biotope composition of an area in response to environmental conditions.LocationBavaria, Federal State of Germany.MethodsBased on the Bavarian biotope mapping data, we modelled the distribution of 29 terrestrial biotopes based on six climate variables and six chemical and four physical soil properties using the species distribution modelling algorithm Maxent.ResultsFor most biotopes, we found that climate variables were more important than soil variables for the biotope distribution and that the area of the predicted biotope distribution was larger than the observed distribution. The potential biotope composition illustrated that while 8% of the area in Bavaria was not sufficiently suitable for any analysed biotope, 92% of the modelled area in Bavaria was suitable for at least one biotope, 84% for two and 77% for at least three biotopes. The difference in suitability between the most suitable biotopes in composition was minor. Further, over one‐quarter of the modelled area was suitable for 6–8 different biotopes.Main ConclusionsOur study showed that considering a composition of potentially suitable biotopes in a raster cell, instead of only the most suitable biotope, provides valuable information to identify conservation priorities and restoration opportunities.