bluuebunny/crossref_metadata_2025_split huggingface dataset:
Identification of the pathogenic relationship between Long COVID and Alzheimer's disease by bioinformatics methods
Authors: Ren Hui , Chen Liang-Liang , Liang Fengjun , Ding Tianhong , Zhang Running , Wei Peifeng , Miao Feng 
Published: February 2023

Abstract
Background
The pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has caused an unprecedented global health crisis. Although many Corona Virus Disease 2019 (COVID-19) patients have recovered, the long-term consequences of SARS-CoV-2 infection are unclear. Several independent epidemiological surveys and clinical studies have found that SARS-CoV-2 infection and Long COVID are closely related to Alzheimer's disease (AD). This could lead to long-term medical challenges and social burdens following this health crisis. However, the mechanism between Long COVID and AD is unknown.
Methods
Genes associated with Long COVID were collected from the database. Two sets of AD-related clinical sample datasets were collected in the Gene Expression Omnibus (GEO) database by limiting screening conditions. After identifying the differentially expressed genes (DEGs) of AD, the significant overlapping genes of AD and Long COVID were obtained by taking the intersection. Then, four kinds of analyses were performed, Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes(KEGG) enrichment analysis, protein-protein interaction (PPI) analysis, identification of hub genes, hub gene verification and transcription factors (TFs) prediction.
Results
A total of 197 common genes were selected for subsequent analysis. GO and KEGG enrichment analysis showed that these genes were mainly enriched in multiple neurodegenerative disease related pathways. In addition, 20 important hub genes were identified using cytoHubba. At the same time, these hub genes were verified in another data set, where 19 hub gene expressions were significantly different in the two diseases and 6 hub genes were significantly different in AD patients of different genders. Finally, we collected 9 TFs that may regulate the expression of these hub genes in the Transcriptional Regulatory Relationships Unraveled by Sentence-based Text mining (TRUSST) database and verified them in the current data set.
Conclusion
This work reveals the common pathways and hub genes of AD and Long COVID, providing new ideas for the pathogenic relationship between these two diseases and further mechanism research.
Preliminary Investigation Into Prospective Applications of Nanosilver, Produced Using Teff (Eragostris Tef) Flour Extract
Authors: KONWARH ROCKTOTPAL , Workie Abie , Moges Abyalew , Seifu Daniel , Elias Dereje , Kebede Nahil , Tsigemariam Yosef , Singh Anand Pratap 
Published: February 2023

Abstract
Silver-based nanomaterials have etched an indelible mark in multiple domains. The green synthesis of silver nanoparticles has received significant attention in comparison to the conventional use of toxic chemicals and reagents in the preparatory stages. In this milieu, the work reported here highlights the use of an aqueous extract of teff (Eragostris tef) flour to prepare silver nanoparticles (TSNPs). The compositional abundance of various phytochemicals, sunlight-induced bio-reduction of silver ions, and subsequent stabilization of the nanostructures by teff’s biomolecules were adroitly exploited. UV-visible spectroscopic analysis was employed to track the evolution of the TSNPs over time and their month-long storage stability. Exhibiting λmax at around 426 nm and energy gap (as revealed by Tauc’s plot) of 2.26 eV, the silver nanomaterial was employed for methylene blue dye degradation (50% degradation in less than 50 min) and DPPH scavenging (IC50 = 243.42 μL containing 410 μg of TSNPs), attesting their catalytic and anti-oxidant potency. On the other hand, anticoagulant action and a concentration-dependent variation were noted for radicle length post germination of Cicer arientinum seeds, treated with the TSNPs. The TSNPs could have profound implications in multiple domains.
Mometasone furoate inhibits the progression of head and neck squamous cell carcinoma via regulating PTPN11
Authors: Qiu Lin , Gao Qian , Tao Anqi , Jiang Jiuhui , Li Cuiying 
Published: February 2023

Abstract
Background: Mometasone Furoate (MF) is a kind of glucocorticoid with extensive pharmacological action including inhibiting tumor progression, however, the role of MF in head and neck squamous cell carcinoma (HNSCC) was still unclear. This study was aim to evaluate the inhibitory effect of MF against HNSCC and investigate the underlying mechanism.
Methods: Cell viability, colony formation, cell cycle and cell apoptosis were detected to explore the effect of MF on HNSCC cells. Xenograft study model was conducted to investigate the effect of MF on HNSCC in vivo. The core target of MF against HNSCC was identified by network pharmacology analysis, TCGA database analysis and real-time PCR. Molecular docking was performed to detect the binding energy. Protein tyrosine phosphatase non-receptor type 11 (PTPN11) overexpressed cells were constructed and then cell viability and the expression levels of proliferation- and apoptosis-related proteins were detected after treated with MF to explore the role of PTPN11 in the inhibitory of MF against HNSCC.
Results: After cells treated with MF, cell viability and the number of colonies were decreased, cell cycle was arrested and cell apoptosis was increased. Xenograft study results showed that MF could inhibit cell proliferation via promoting cell apoptosis in vivo. PTPN11 was the core target of MF against HNSCC via network pharmacology analysis, TCGA database analysis and real-time PCR. Molecular docking results revealed PTPN11 exhibited the strongest binding ability to MF. Finally, MF could attenuate the effect of increased cell viability and decreased cell apoptosis caused by PTPN11 overexpression, suggesting that MF could inhibit the progression of HNSCC by regulating PTPN11.
Conclusion: MF targeted PTPN11 to promote cycle arrest and cell apoptosis subsequently performed an effective anti-tumor activity.
Impact of H9N2 Infection on the Pathological and Immunological Effects of the IBD Vaccine in Chicks: A Study of Vaccine Interaction and Immune Response
Authors: Khalil N. W. , Elshorbagy M. A. , Elboraay E. M. , Helal A. M. 
Published: February 2023

Abstract
Background:
This study aimed to investigate the impact of H9N2 infection on the pathological and immunological effects of the IBD vaccine, and how their interaction affects the immune response to other vaccines. The objective was to determine if H9N2 infection before IBD vaccination influences the efficacy of the vaccine and if it impacts other vaccines' immune response. One hundred SPF chicks were divided into four groups in four isolators that were either H9N2 challenged, IBD vaccinated, both, or neither. Blood samples were collected weekly to measure antibody titers against IBDV, ND, H5, and H9. Cloacal swabs were taken on the 4th and 8th days post-vaccination to quantify the IBD vaccine shedding. Five chicks per group were euthanized at the 17th, 21st, and 25th days old to calculate immune organ indices and conduct histopathological examinations.
Results:
The study's results revealed that pre-challenge with H9N2 affected many factors. The dual-treated group had a significant decrease in the bursal index compared to the other groups. The bursal microscopical score and renal lesion score of the dual-treated group recorded a significant rise when compared to the other groups. The shedding of the IBD vaccine was negatively affected by pre-infection with H9N2, so the dual-treated group recorded a significant decrease in IBDV shedding in comparison to the IBD-vaccinated group. Additionally, the immune response against the IBD vaccine significantly decreased in the dual-treated group. Finally, there was an increase in the immunosuppressive effect of the IBD vaccine, which reflected a significant decline in the antibody titers against ND and H5 in the dual-treated group.
Conclusion:
The study suggests that early diagnosis of H9N2 before IBD vaccination is crucial. Pre-challenge with H9N2 significantly impacted the pathological and immunological effects of the IBD vaccine. The results showed a decrease in IBD vaccine shedding and immune response against the IBD vaccine in the dual-treated group. The immunosuppressive effect of the IBD vaccine also affected the immune response to other vaccines, as shown by the significant decline in the antibody titers against ND and H5 in the dual-treated group. Therefore, the study highlights the importance of considering H9N2 infection status before administering the IBD vaccine to ensure optimal immune response and protection.
Modularized and Scalable Compilation for Double Quantum Dot Quatum Computing
Authors: He Run-Hong , Xu Xu-Sheng , Byrd Mark , Wang Zhao-Ming 
Published: February 2023

Abstract
Any quantum program on a realistic quantum device must be compiled into an executable form while taking into account the underlying hardware constraints. Stringent restrictions on architecture and control imposed by physical platforms make this very challenging. In this paper, based on the quantum variational algorithm, we propose a novel scheme to train an Ansatz circuit and realize high-fidelity compilation of a set of universal quantum gates for singlet-triplet qubits in semiconductor double quantum dots, a fairly heavily constrained system. Furthermore, we propose a scalable architecture for a modular implementation ofquantum programs in this constrained systems and validate its performance with two representative demonstrations, Grover's algorithm for the database searching (static compilation) and a variant of variational quantum eigensolver for the Max-Cut optimization (dynamic compilation). Our methods are potentially applicable to a wide range of physical devices. This work constitutes an important stepping-stone for exploiting the potential for advanced and complicated quantum algorithms on near-term devices.
Echo2Pheno: A deep learning application to uncover echocardiographic phenotypes in conscious mice
Authors: Bukas Christina , Galter Isabella , Silva-Buttkus Patricia , Fuchs Helmut , Maier Holger , Gailus-Durner Valerie , Müller Christian L. , Angelis Martin Hrabě , Piraud Marie , Spielmann Nadine 
Published: February 2023

Abstract
Echocardiography, a rapid and cost-effective imaging technique, assesses cardiac function and structure. Despite its popularity in cardiovascular medicine and clinical research, image-derived phenotypic measurements are manually performed, requiring expert knowledge and training. Notwithstanding great progress in deep learning applications in small animal echocardiography, the focus has so far only been on images of anaesthetized rodents. We present here a new algorithm specifically designed for echocardiograms acquired in conscious mice called Echo2Pheno, an automatic statistical learning workflow for analyzing and interpreting high-throughput non-anesthetized transthoracic murine echocardiographic images in the presence of genetic knockouts. Echo2Pheno comprises a neural network module for echocardiographic image analysis and phenotypic measurements, including a statistical hypothesis-testing framework for assessing phenotypic differences between populations. Using 2159 images on 16 different knockout mouse strains of the German Mouse Clinic, Echo2Pheno accurately confirms known cardiovascular genotype–phenotype relationships (e.g. Dystrophin) and discovers novel genes (e.g. CCR4-NOT transcription complex subunit 6-like, Cnot6l, and synaptotagmin-like protein 4, Sytl4), which cause altered cardiovascular phenotypes, as verified by H&E-stained histological images. Echo2Pheno provides an important step toward automatic end-to-end learning for linking echocardiographic readouts to cardiovascular phenotypes of interest in conscious mice.
Quick insights into whisky – investigating rapid and efficient methods for sensory evaluation and chemical analysis
Authors: Haug Helen , Grasskamp Andreas T. , Singh Satnam , Strube Andrea , Sauerwald Tilman 
Published: February 2023

Abstract
The evaluation of aroma properties of beverages both analytically as well as with human sensory is a challenging task and most often related to time- and cost-intensive analyses. Whisky is a spirit offering a wide variety of aroma impressions caused by a complex mixture of aroma active compounds. In the present study, methods for the efficient evaluation of aroma characteristics are evaluated for 16 whisky samples of different origins (Scotch and American). Rate all that apply (RATA) was applied as a rapid sensory method for the sensory evaluation of whiskies. Sensory evaluation of the samples led to the determination of eight significant aroma attributes: Caramel / cream caramel / toffee, vanilla, (canned) peach, phenolic, smoky, fruity, flowery and (fermented) apple / cider. Chemical analysis was conducted by stir bar sorptive extraction (SBSE) coupled to gas chromatography mass spectrometry in combination with an in-house data processing tool for automated analyte identification. Through chemical analysis of the whisky samples and automated compound identification, over 200 mostly aroma-active volatiles were detected. To test both approaches for their potential for sample classification, a simple classification problem (Scotch vs. American) was applied. Linear discriminant analysis (LDA) indicates both that sensory evaluation by RATA (97.86%) and the applied analytical procedure (96.94%) are suitable for the distinction between the two whisky types. For both approaches, markers were identified for the classification. These investigations build a solid foundation for the implementation of a versatile platform for rapid and efficient aroma evaluation for various foodstuffs and beverages.
Contribution Of Hydrogeochemical And Statistic Tools To The Acquisition Of Water Quality In The North-Eastern Of Algeria: Annaba-El Tarf
Authors: BOUNAB Samia , SEDRATI Nassima , KHEMMOUDJ Kaddour , MAJOUR Habiba 
Published: February 2023

Abstract
This work was conducted on 34 samples spread over 4 areas (Bouteldja, El Tarf, Les Salines et Barrahal) tapping the shallow and deep aquifer of groundwater in the North-eastern Algeria (Annaba-El Tarf), one sampling campaign was conducted in 2017 to describe the chemistry of the water and its interaction with the rock. The interpretation of the geological-geophysical and hydrogeological data allowed us to highlight two principal aquifers. The first consists of the sandy clay formations up to 18 m thick. It is generally free except at a few points where it is captive under clay levels. The second is more important localized in the gravel layer below first aquifer; it covers the entire study area. It is formed by Plio-Quaternary gravels, sands and pebbles and is contained in the collapse basin. The methodology used for the processing of the analysis results is based on multivariate statistical methods and characteristic ratios coupled with hydrochemical methods, as well as the calculation of saturation indices. The results show that the water quality is controlled both by the dissolution of the minerals of the evaporite formations and those of the carbonate formations, alteration of silicates and marine invasion.
Multiple antiplatelet therapy in ischemic stroke already on antiplatelet agents
Authors: Kim Tae Jung , Lee Ji Sung , Yoon Jae Sun , Park Soo-Hyun , Oh Mi Sun , Jung Keun-Hwa , Yu Kyung-Ho , Lee Byung-Chul , Ko Sang-Bae , Yoon Byung-Woo 
Published: February 2023

Abstract
Background: Optimal antiplatelet strategy for patients with ischemic stroke who were already on single antiplatelet therapy (SAPT) remains to be elucidated. This study aimed to evaluate the effect of different antiplatelet regimens on vascular and safety outcomes at 1 year after non-cardioembolic stroke in patients previously on SAPT.
Methods: We identified 9,284 patients with acute non-cardioembolic ischemic stroke that occurred on SAPT using linked data. Patients were categorized into three groups according to antiplatelet strategy at discharge: 1) SAPT, 2) dual antiplatelet therapy (DAPT), and 3) triple antiplatelet therapy (TAPT). One-year outcomes included recurrent ischemic stroke, composite outcomes (recurrent ischemic stroke, myocardial infarction, intracerebral hemorrhage, and death), and major bleeding.
Results: Of 9,284 patients, 5,565 (59.9%) maintained SAPT, 3,638 (39.2%) were treated with DAPT, and 81 (0.9%) were treated with TAPT. Multiple antiplatelet therapy did not reduce the risks of 1-year recurrent stroke (DAPT, hazard ratio [HR] 1.08 [95%confidence interval, CI, 0.92–1.27], P= 0.339; TAPT, HR, 0.71 [95%CI, 0.27–1.91], P = 0.500) and 1-year composite outcome (DAPT, HR, 1.09 [95%CI, 0.68–1.97], P = 0.592; TAPT HR, 1.46 [95%CI, 0.68–1.97], P = 0.592). However, the TAPT groups showed an increased risk of major bleeding complications (DAPT, HR, 1.23 [95%CI, 0.89–1.71], P = 0.208; TAPT, HR, 4.65 [95%CI, 2.01–10.74], P < 0.001).
Conclusions: Additional use of antiplatelet agents in patients with non-cardioembolic ischemic stroke who were already on SAPT did not reduce the 1-year incidence of vascular outcomes, although it increased the risk of bleeding complications.
Immunotherapy with cure potential of multi-drug resistant hematologic malignancies using IL-2 preactivated intentionally mismatched donor lymphocytes
Authors: Slavin Shimon 
Published: February 2023

Abstract
Purpose: Unfortunately, cure of multi-drug resistant (MDR) hematologic malignancies remains an unmet need. Donor lymphocyte infusion (DLI) following allogeneic stem cell transplantation (SCT) can sometimes eliminate multi-drug resistant leukemia but at a risk of acute and chronic graft-vs-host disease (GVHD) and procedure-related toxicity. Supported by pre-clinical experiments in animal models, we hypothesized that immunotherapy induced by non-engrafting intentionally mismatched IL-2 activated killers (IMAK) including both T & NK cells could induce safer, faster and much more effective immunotherapy while avoiding the need for SCT and the risks of GVHD.
Methods: IMAK treatment was applied in 33 patients with MDR hematologic malignancies conditioned with cyclophosphamide 1,000mg/m^2 based protocol. Haploidentical or unrelated donor lymphocytes were preactivated with IL-2 6,000 IU/ml for 4 days. IMAK was combined with Rituximab in 12/23 patients with CD20+ B cells.
Results: A total of 23/33 patients with MDR (4 failing SCT) achieved complete remission (CR).
First patient currently 30 years with no further treatment and 5 observed for >5 years (2 AML; 2 multiple myeloma, 1 ALL & 1 NHL) can be considered cured. No patient developed >grade 3 toxicity or GVHD. No residual male cells were detectable among 6 females treated with male cells beyond day +6, confirming that GVHD was prevented by consistent early rejection of donor lymphocytes.
Conclusions: We hypothesize that safe and superior immunotherapy of MDR with cure potential may be accomplished by IMAK, most probably in patients with low tumor burden, but that remains to be confirmed by future clinical trials.
The Experimental Design Approach to Removal of Endocrine Disrupting Compounds from Domestic Wastewater by Electrooxidation Process
Authors: SÖZÜDOĞRU Onur , KOÇOĞLU Elif Seda , YILMAZ Alper Erdem , BAKIRDERE Sezgin , KOMESLİ Okan Tarık 
Published: February 2023

Abstract
In this study, the treatment performance of the process in the removal of Endocrine Disrupting Compounds (EDCs) from domestic wastewater by a laboratory-scale electrooxidation process using Ti/IrO₂/RuO₂ electrodes as an anode was evaluated using response surface method (RSM). The effect of pH, current density, and flow rate on the electrochemical treatment of 17α-ethinylestradiol, β-estradiol, triclosan, and estrone, which are often present in wastewater, has been studied. Using Box-Behnken Design (BBD), the parameters influencing the removal efficiencies were optimized for the Electrooxidation process (EOP), and the models created essential second-order quadratic models for the EOP process. The Response Surface Method yielded results that reasonably agreed with the measured values. The maximum removals of triclosan, 17α-ethinylestradiol, and β-estradiol were attained at 92,90%, 97,76%, and 95,36% respectively, under experimental conditions optimized pH= 3,68, current density= 20 A and flow rate= 8,83 mL/min for EOP. Removal efficiencies have achieved their maximum levels at low pH, high current density, and low flow rate. At the same time, the electrooxidation method could not completely remove the estrone.
Synthesis and Characteristics of Graphene-Graphene Oxide Material Obtained by an Underwater Impulse Direct Current Discharge
Authors: Sirotkin Nikolay , Korolev Viktor 
Published: February 2023

Abstract
This work proposed a novel approach, in which an impulse underwater discharge was used to produce graphene-graphene oxide material in distilled water. The characteristics of this discharge were presented, including electrical parameters, plasma composition, and electron concentration. Graphene-based material produced using this approach can be evenly dispersed in water without the use of a surfactant or stabilizer, and is suitable for storage at room temperature. Ultraviolet–visible spectroscopy was employed to analyze the optical properties of the graphene-based structures. Scanning electron microscopy was adopted to explore the morphology and size of the particles. The FTIR spectroscopy confirms the formation of graphene oxide. The Raman spectroscopy demonstrates the formation of a graphene-containing multilayer material. The results of this study confirmed that graphene-based material production by impulse underwater discharge is a low-cost, fast, and effective manufacturing method.
Incidence and clinical relevance of postoperative diarrhea after minimally invasive gastrectomy for gastric cancer: A single institution retrospective study of 1476 patients
Authors: Fujita Masahiro , Nakauchi Masaya , Suzuki Kazumitsu , Serizawa Akiko , Akimoto Shingo , Tanaka Tsuyoshi , Shibasaki Susumu , Inaba Kazuki , Tochio Takumi , Hirooka Yoshiki , Uyama Ichiro , Suda Koichi 
Published: February 2023

Abstract
Purpose
Postoperative diarrhea (PD) remains one of the significant complications. Only a few studies focused on PD after minimally invasive surgery. We aimed to investigate PD after minimally invasive gastrectomy for gastric cancer.
Methods
A total of 1476 consecutive patients with gastric cancer undergoing laparoscopic or robotic gastrectomy between 2009 and 2019 at our institution were retrospectively reviewed. PD was defined as continuous diarrhea for ≥ 2 days, positive stool culture, or positive clostridial antigen test. The incidence, causes, and related clinical factors were analyzed.
Results
Of the 1476 patients, the median age was 69 years. Laparoscopic and robotic approaches were performed in 1072 (72.6%) and 404 (27.4%), respectively. Postoperative complications with Clavien–Dindo classification grade of ≥ IIIa occurred in 108 (7.4%) patients. PD occurred in 89 (6.0%) patients. Of the 89 patients with PD, Clostridium difficile, enteropathogenic Escherichia coli, and methicillin-resistant Staphylococcus aureus were detected in 24 (27.0%), 16 (33.3%), and 7 (14.6%) patients, respectively. Multivariate analysis revealed that age ≥ 75 years (OR 1.62, 95% CI [1.02–2.60], p = 0.042) and postoperative complications (OR 6.04, 95% CI [3.54–10.32], p < 0.001) were independent risk factors for PD. In patients without complications, TG (OR 1.88) and age of ≥ 75 years(OR 1.71) were determined as independent risk factors.
Conclusion
The incidence of PD following minimally invasive gastrectomy for gastric cancer was 6.0%. Older age and TG were obvious risk factors in such a surgery, with the latter being a significant risk even in the absence of complications.