bluuebunny/crossref_metadata_2025_split huggingface dataset:
OsWR2 recruits HDA704 to regulate the deacetylation of H4K8ac in the promoter of OsABI5 in response to drought stress
Authors: Guo Yalu , Tan Yiqing , Qu Minghao , Hong Kai , Zeng Longjun , Wang Lei , Zhuang Chuxiong , Qian Qian , Hu Jiang , Xiong Guosheng 
Published: March 2023

ABSTRACTDrought stress is a major environmental factor that limits the growth, development, and yield of rice (Oryza sativa L.). Histone deacetylases (HDACs) are involved in the regulation of drought stress responses. HDA704 is an RPD3/HDA1 class HDAC that mediates the deacetylation of H4K8 (lysine 8 of histone H4) for drought tolerance in rice. In this study, we show that plants overexpressing HDA704 (HDA704‐OE) are resistant to drought stress and sensitive to abscisic acid (ABA), whereas HDA704 knockout mutant (hda704) plants displayed decreased drought tolerance and ABA sensitivity. Transcriptome analysis revealed that HDA704 regulates the expression of ABA‐related genes in response to drought stress. Moreover, HDA704 was recruited by a drought‐resistant transcription factor, WAX SYNTHESIS REGULATORY 2 (OsWR2), and co‐regulated the expression of the ABA biosynthesis genes NINE‐CIS‐EPOXYCAROTENOID DIOXYGENASE 3 (NCED3), NCED4, and NCED5 under drought stress. HDA704 also repressed the expression of ABA‐INSENSITIVE 5 (OsABI5) and DWARF AND SMALL SEED 1 (OsDSS1) by regulating H4K8ac levels in the promoter regions in response to polyethylene glycol 6000 treatment. In agreement, the loss of OsABI5 function increased resistance to dehydration stress in rice. Our results demonstrate that HDA704 is a positive regulator of the drought stress response and offers avenues for improving drought resistance in rice.
Factors related to parenting styles of Chinese mothers of children with and without intellectual disability
Authors: Su H. , Cuskelly M. , Gilmore L. 
Published: March 2023

AbstractBackgroundParenting styles play a crucial role in children's development. However, approaches to parenting children with intellectual disability (ID) beyond Western cultures have been underexamined. This study compared the self‐reported parenting styles of Chinese mothers of children with and without ID and examined some factors that might be related.MethodsChinese mothers of children with ID (n = 173) and mothers of typically developing children (n = 119) completed measures of their parenting style, parenting stress, parenting sense of competence and family support.ResultsBoth groups endorsed similar levels of authoritative parenting, but mothers of children with ID were more likely to report adopting strategies aligned with authoritarian parenting. For mothers in the ID group, family support moderated the effects of parenting stress and parenting sense of competence on authoritative parenting. Parenting stress and parenting sense of competence, respectively, predicted authoritarian parenting for mothers of children with and without ID.ConclusionsThis study highlights the importance of reducing parenting stress, strengthening parenting sense of competence and providing family support in order to promote optimal parenting styles for Chinese mothers of children with ID.
Effects of bone type and occlusal loading pattern on bone remodeling in implant‐supported single crown: A finite element study
Authors: Rungsiyakull Pimduen , Rungsiyakull Chaiy , Monstaporn Montip , Sae‐lee Daraporn , Elsaka Shaymaa 
Published: March 2023

AbstractPurposeTo assess the influence of bone types and loading patterns on the remodeling process over 12 months according to the variations in stress, strain, strain energy density (SED), and density allocation in the bone of implant‐supported single crown.Materials and methodsA three‐dimensional finite element of a single crown implant was modeled in five different bone types (D1–D4, and grafted bone). A 200 N load was applied on an implant crown with three occlusal loading patterns (nonfunctional contact, functional contact at center, and at 2‐mm offset loading). During the first 12 months after implant placement, the SED was employed as a mechanical stimulus to simulate cortical and cancellous bone remodeling.ResultsFunctional contact at 2‐mm offset loading led to a higher bone remodeling rate and stress compared to functional contact at center and nonfunctional contact. Under 2‐mm offset loading, the greatest remodeling rate after 12 months was achieved with D3 and D4, D2, grafted, and D1 cortical bone with an average peri‐implant density of 1.95, 1.77, 1.56, and 1.50 g/cm3, respectively. Meanwhile, the highest von Mises stresses were found in D4 (22.2 MPa) and D3 (21.9 MPa) bones.ConclusionsA greater stress concentration and remodeling rate were found when an off‐axial load was applied on an implant placed in low bone density. Although the fastest remodeling processes resulting in increased bone density and strength were found in D3 and D4 bone types with greater off‐axial loading that may provide greater bone engagement, it could increase stress concentrations that are susceptible to inducing implant failure.
Pre‐conditioning of gingival epithelial cells with sub‐apoptotic concentrations of curcumin prevents pro‐inflammatory cytokine release
Authors: Grant Melissa M. , Scott Ann E. , Matthews John B. , Griffiths Helen R. , Chapple Iain L. C. 
Published: March 2023

AbstractBackground and ObjectivePlaque‐induced gingival inflammation (gingivitis) is ubiquitous in humans. The epithelial barrier reacts to the presence of oral bacteria and induces inflammatory cascades. The objective of this study was to investigate the mechanism by which the small molecule micronutrient curcumin could decrease inflammatory response in vitro to oral bacterium heat‐killed Fusobacterium nucleatum as curcumin could be a useful compound for combatting gingivitis already consumed by humans.MethodsH400 oral epithelial cell line was pre‐conditioned with curcumin and the production of cytokines was measured by enzyme‐linked immunosorbent assay (ELISA) and translocation of transcription factors was used to monitor inflammatory responses. Haem oxygenase (HO‐1) expression and molecules that HO‐1 releases were evaluated for their potential to reduce the quantity of cytokine production. Immunofluorescence microscopy and Western blotting were used to evaluate changes in transcription factor and enzyme location.ResultsPre‐conditioning of H400 cells with a sub‐apoptotic concentration of curcumin (20 μM) attenuated secretion of Granulocyte‐Macrophage – Colony‐Stimulating Factor (GM‐CSF) and reduced NFkB nuclear translocation. This pre‐conditioning caused an increase in nuclear Nrf2; an initial drop (at 8 h) followed by an adaptive increase (at 24 h) in glutathione; and an increase in haem oxygenase (HO‐1) expression. Inhibition of HO‐1 by SnPPIX prevented the curcumin‐induced attenuation of GM‐CSF production. HO‐1 catalyses the breakdown of haem to carbon monoxide, free iron and biliverdin: the HO‐1/CO anti‐inflammatory pathway. Elevations in carbon monoxide, achieved using carbon monoxide releasing molecule‐2 (CORM2) treatment alone abrogated F. nucleatum‐induced cytokine production. Biliverdin is converted to bilirubin by biliverdin reductase (BVR). This pleiotropic protein was found to increase in cell membrane expression upon curcumin treatment.ConclusionCurcumin decreased inflammatory cytokine production induced by Fusobacterium nucleatum in H400 oral epithelial cells. The mechanism of action appears to be driven by the increase of haem oxygenase and the production of carbon monoxide.
Reliability of parental reporting of child snoring in children referred for obstructive sleep apnea
Authors: Bokov Plamen , Dudoignon Benjamin , Spruyt Karen , Delclaux Christophe 
Published: March 2023

SummaryDespite the high number of studies based on subjective reports of snoring, self‐reported snoring has hardly been validated at all. As there is no “gold‐standard” for objective snoring measurements, studies must evaluate whether the presence of snoring based on parental judgement is linked to objective measurements of nasal and/or pharyngeal obstruction in children referred for obstructive sleep apnea. A total of 146 children (median age 11 years) underwent polysomnography (with snoring recording using nasal cannula signal), acoustic rhinometry and pharyngometry, while their parents filled out the Spruyt–Gozal questionnaire assessing both frequency and loudness of subjective snoring. Three categories were further differentiated (null, low and high) for both frequency and loudness. The apnea–hypopnea index was significantly different in the three groups for both frequency (p = 0.04) and loudness (p = 0.01) of subjective snoring. Children in the low or high groups (frequency or loudness), compared with those in the null group, experienced a decline in both pharyngeal (sitting and supine positions) and nasopharyngeal (supine position) volumes (frequency, pharynx sitting: p = 0.03; supine: 0.005 and nasopharynx: p = 0.002; loudness, p = 0.03; p = 0.007 and p = 0.03; three group comparisons). Objective snoring frequency during the night obtained with cannula was weakly related to loudness of subjective snoring but not to subjective snoring frequency during the week, and was biased by nasal obstruction. In conclusion, our study showed that parental assessment of snoring is related to a reduction in both pharyngeal and nasopharyngeal volumes in snorers, arguing for the adequacy of their evaluation of both snoring frequency and loudness.
Begomoviral βC1 orchestrates organellar genomic instability to augment viral infection
Authors: Nair Ashwin , Harshith Chitthavalli Y. , Narjala Anushree , Shivaprasad Padubidri V. 
Published: March 2023

SUMMARYChloroplast is the site for transforming light energy to chemical energy. It also acts as a production unit for a variety of defense‐related molecules. These defense moieties are necessary to mount a successful counter defense against pathogens, including viruses. Previous studies indicated disruption of chloroplast homeostasis as a basic strategy of Begomovirus for its successful infection leading to the production of vein‐clearing, mosaic, and chlorotic symptoms in infected plants. Although begomoviral pathogenicity determinant protein Beta C1 (βC1) was implicated for pathogenicity, the underlying mechanism was unclear. Here we show that, begomoviral βC1 directly interferes with the host plastid homeostasis. βC1 induced DPD1, an organelle‐specific nuclease, implicated in nutrient salvage and senescence, as well as modulated the function of a major plastid genome maintainer protein RecA1, to subvert plastid genome. We show that βC1 was able to physically interact with bacterial RecA and its plant homolog RecA1, resulting in its altered activity. We observed that knocking‐down DPD1 during virus infection significantly reduced virus‐induced necrosis. These results indicate the presence of a strategy in which a viral protein alters host defense by targeting modulators of chloroplast DNA. We predict that the mechanism identified here might have similarities in other plant–pathogen interactions.
CD4
+
T cells produce GM-CSF and drive immune-mediated glomerular disease by licensing monocyte-derived cells to produce MMP12
Authors: Paust Hans-Joachim , Song Ning , De Feo Donatella , Asada Nariaki , Tuzlak Selma , Zhao Yu , Riedel Jan-Hendrik , Hellmig Malte , Sivayoganathan Amirrtavarshni , Peters Anett , Kaffke Anna , Borchers Alina , Wenzel Ulrich O. , Steinmetz Oliver M. , Tiegs Gisa , Meister Elisabeth , Mack Matthias , Kurts Christian , von Vietinghoff Sibylle , Lindenmeyer Maja T. , Hoxha Elion , Stahl Rolf A. K. , Huber Tobias B. , Bonn Stefan , Meyer-Schwesinger Catherine , Wiech Thorsten , Turner Jan-Eric , Becher Burkhard , Krebs Christian F. , Panzer Ulf 
Published: March 2023

GM-CSF in glomerulonephritis

Despite glomerulonephritis being an immune-mediated disease, the contributions of individual immune cell types are not clear. To address this gap in knowledge, Paust
et al
. characterized pathological immune cells in samples from patients with glomerulonephritis and in samples from mice with the disease. The authors found that CD4+ T cells producing granulocyte-macrophage colony-stimulating factor (GM-CSF) licensed monocytes to promote disease by producing matrix metalloproteinase 12 and disrupting the glomerular basement membrane. Targeting GM-CSF to inhibit this axis reduced disease severity in mice, implicating this cytokine as a potential therapeutic target for patients with glomerulonephritis. —CM
Plasma Progerin in Patients With Hutchinson-Gilford Progeria Syndrome: Immunoassay Development and Clinical Evaluation
Authors: Gordon Leslie B. , Norris Wendy , Hamren Sarah , Goodson Robert , LeClair Jessica , Massaro Joseph , Lyass Asya , D’Agostino Ralph B. , Tuminelli Kelsey , Kieran Mark W. , Kleinman Monica E. 
Published: March 2023

Background:
Hutchinson-Gilford progeria syndrome (HGPS) is an ultrarare, fatal, premature aging disease caused by a toxic protein called progerin. Circulating progerin has not been previously detected, precluding research using readily available biological samples. This study aimed to develop a plasma progerin assay to evaluate progerin’s quantity, response to progerin-targeted therapy, and relationship to patient survival.

Methods:
Biological samples were collected by The Progeria Research Foundation Cell and Tissue Bank from a non-HGPS cohort cross-sectionally and a HGPS cohort longitudinally. HGPS donations occurred at baseline and intermittently while treated with farnesylation inhibitors lonafarnib±pravastatin and zoledronate, within 3 sequential open-label clinical trials at Boston Children’s Hospital totaling >10 years of treatment. An ultrasensitive single-molecule counting progerin immunoassay was developed with prespecified performance parameters. Intra- and interpatient group statistics were descriptive. The relationship between progerin and survival was assessed by using joint modeling with time-dependent slopes parameterization.

Results:

The assay’s dynamic detection range was 59 to 30 000 pg/mL (
R
2
=0.9987). There was no lamin A cross-reactivity. Mean plasma progerin in non-HGPS participants (n=69; 39 male, 30 female; age, 0.2–71.3 years) was 351±251 pg/mL, and in drug-naive participants with HGPS (n=74; 37 female, 37 male; age, 2.1–17.5 years) was 33 261±12 346 pg/mL, reflecting a 95-fold increase in affected children (
P
<0.0001). Progerin levels did not differ by sex (
P
=0.99). Lonafarnib treatment resulted in an average per-visit progerin decrease from baseline of between 35% to 62% (all
P
<0.005); effects were not augmented by adding pravastatin and zoledronate. Progerin levels fell within 4 months of therapy and remained lower for up to 10 years. The magnitude of progerin decrease positively associated with patient survival (
P
<0.0001; ie, 15 000 pg/mL decrease yields a 63.9% decreased risk of death). For any given decrease in progerin, life expectancy incrementally increased with longer treatment duration.

Conclusions:
A sensitive, quantitative immunoassay for progerin was developed and used to demonstrate high progerin levels in HGPS plasma that decreased with lonafarnib therapy. The extent of improved survival was associated with both the magnitude of progerin decrease and duration at lower levels. Thus, plasma progerin is a biomarker for HGPS whose reduction enables short- and long-term assessment of progerin-targeted treatment efficacy.

Registration:

URL:
https://www.clinicaltrials.gov
. Unique identifiers: NCT00879034 and NCT00916747.
A Bodily Haunting
Authors: Simpson Hannah 
Published: March 2023

Abstract
The woman’s wordless scream in Not I and Happy Days acts as a spectral-yet-embodied rendering of unspoken and apparently unspeakable sexual trauma. If trauma symptomology is itself a form of bodily haunting—the past intruding into the present—the wordless scream performs this phenomenon on Beckett’s stage, as a disruptive return of the repressed through the body itself. This essay explores how the performed scream returns embodied trauma to embodied expression in Not I and Happy Days, emphasising the voice as a simultaneously spectral yet profoundly corporeal force. It then examines the potential therapeutic effect of the scream in performance, drawing on a range of actor testimonies.
Love and Gender Relations from the Perspective of Familism: Based on Fieldwork in Contemporary Rural North Zhejiang
Authors: Chen (陈云龙) Yunlong 
Published: March 2023

Abstract
In response to the academic debate about the “individualization” of the patterns of love and marriage in contemporary China, this article takes the spousal selection and marriage and love practices of peasants in northern Zhejiang since China’s reform and opening up as the basis of field study, and explores the roles and functions of love and gender relations and their relationship to ethical factors in the overall process of “spousal selection→marriage→family→giving birth.” We also find that although the “love” factor has become more and more important in the marriage practice of recent generations (especially among rural women) and has even become an indispensable key to starting marital family life, it has not fully established its own independent space and value. It has in fact been boosted, guided, and controlled by, and incorporated into, the traditional marriage and family pattern. This traditional pattern, with its own unique resilience, has integrated love into the family life cycle, gradually pulling it into the orbit of traditional marriage and family, and successfully carrying on the “sacred undertaking” of the ancestors. This overall characteristic is more pronounced in developed eastern coastal villages (among young women) than in less developed central and western rural areas (among young women). In terms of theory and methodology, the “individualization” of love and marriage among contemporary Chinese peasants needs to be put back into the framework of “familism” for in-depth reflection, rather than simply being understood from the standpoint of “individualization” and from the binary opposition between “individualism” and “familism.”
The safety and efficacy of sequential intramuscular/oral ziprasidone treatment of acute episode in patients with schizophrenia: a multicenter, open-labeled study
Authors: Wu Yaxue , Li Yanli , Liang Weiye , Bai Luyuan , Yu Jianjin , Li Keqing , Zhang Yunshu , Guo Yanmei , Liu Zenglong , Wang Jian , Zhang Congpei , Wang Xijin , Xu Jia , Liu Liping , Li Juan , Yang Fude 
Published: March 2023

Abstract
Background
Ziprasidone mesylate injection is an atypical antipsychotic drug which is recently approved in China. In combination with its oral formulation, sequential therapy with ziprasidone brings new interventions to patients with agitation in the acute phase of schizophrenia. The purpose of this 7-day multicenter study conducted in China was to evaluate the efficacy and safety of ziprasidone sequential treatment through intramuscular/oral routes in agitated patients with schizophrenia.

Methods
A total of 95 patients were enrolled from three centers in this study. The study duration was 7 days. In the first 3 days, subjects were administered an intramuscular injection of ziprasidone 10–40 mg daily and started sequentially with oral ziprasidone 40–80 mg at dinner (or lunch) from the day of the last intramuscular injection. In the following 4 days, according to the severity of the symptoms and the drug response, 120–160 mg of ziprasidone was orally administered daily. In total, six visits were scheduled to assess the Positive and Negative Syndrome Scale (PANSS), the Behavioral Activity Rating Scale (BARS), the Clinical Global Impression of Severity (CGI-S), and Improvement (CGI-I) scores throughout the procedure. Lastly, adverse events were recorded during treatment.

Results
Out of the 95 patients that were enrolled, 83 cases were effectively completed. Visits 3, 4, 6, PANSS, and PANSS-excited component (PANSS-EC) subscale points, and Visit 2–Visit 6 viewpoints, BARS scale points, and baseline scores denote a progressive downward trend (P < 0.001). In this study, 62 adverse events were reported. The most common adverse events were extrapyramidal symptoms (EPS) (23 cases) and excessive sedation(10 cases), and 13 cases of prolonged QTc interval were reported.

Conclusions
Ziprasidone IM demonstrated significant and rapid reduction in agitation, and sequential oral formulation keep stability and continuation of the treatment can further ensure efficacy. Ziprasidone sequential therapy may provide a new approach to acute agitation in schizophrenic patients.

Trial registration
The Chinese Clinical Trials Registry; URL: https://www.chictr.org.cn: ChiCTR-OIC-16007970.
Characteristics of submucosal leiomyomas that could cause severe hemorrhage with relugolix: an observational study
Authors: Wada Yoshimitsu , Takei Yuji , Minezumi Takumi , Hirashima Hiroto , Fujiwara Hiroyuki 
Published: March 2023

Abstract
Background
Relugolix, an oral gonadotrophin-releasing hormone receptor antagonist, was launched in Japan in 2019. Although there have been several studies on relugolix for leiomyomas, few have focused on submucosal leiomyomas. Submucosal leiomyomas cause bleeding more frequently than leiomyomas in other locations. There is only one case report described a patient treated for a submucosal leiomyoma with relugolix who developed severe hemorrhage. However, it remains unclear which characteristics of submucosal leiomyomas can lead to severe hemorrhage. Thus, the aim of this study was to investigate the characteristics of submucosal leiomyomas that would cause severe hemorrhage when treated with relugolix.

Methods
We retrospectively reviewed records of patients who underwent treatment for submucosal leiomyoma with relugolix (40 mg once daily for up to 6 months) in our institute between December 2019 and September 2021. We evaluated the clinical course and characteristics of submucosal leiomyoma in patients who developed severe hemorrhage.

Results
A total of 17 patients were treated for submucosal leiomyoma with relugolix. Two patients developed severe hemorrhage and required emergent surgery and blood transfusions. Only those two of the 17 patients had a submucosal leiomyoma of the International Federation of Gynecology and Obstetrics (FIGO) type 0, which has a stalk. In the remaining 15 patients who had FIGO type 1 or 2 leiomyoma, hemorrhage did not occur.

Conclusions
Our study suggests that the use of relugolix for FIGO type 0 leiomyomas may be associated with a risk of hemorrhage. However, relugolix may be a safe and effective treatment option for patients with FIGO type 1 or 2 leiomyomas.