bluuebunny/crossref_metadata_2025_split huggingface dataset:
Lentiviral Micro-dystrophin Gene Treatment into Late-stage mdx Mice
for Duchenne Muscular Dystrophy Disease
Authors: Eren Selen Abanuz , Tastan Cihan , Karadeniz Kevser Buse , Turan Raife Dilek , Cakirsoy Didem , Kancagi Derya Dilek , Yilmaz Sevdican Ustun , Oztatlici Mustafa , Oztatlici Hulya , Ozer Samed , Tumentemur Gamze , Baykal Ahmet Tarık , Ovali Ercument 
Published: April 2023

Aim:
Duchenne Muscular Dystrophy (DMD) results in a deficiency of dystrophin expression
in patient muscle fibers, leading to progressive muscle degeneration. Treatment of DMD has undertaken
current transformation with the advancement of novel gene therapy and molecular biology
techniques, which are secure, well-tolerated, and effective therapeutic approaches.

Introduction:
DMD gene therapies have mainly focused on young DMD patients as in vivo animal
model trials have been performed in 0–1-month DMD mice. However, it has not yet been answered
how micro-dystrophin encoding lentiviral treatment affects Dystrophin expression and DMD symptoms
in 10-month mdx mice.

Methods:
We planned to integrate the micro-Dystrophin gene sequence into the muscle cells by viral
transfer, using micro-Dystrophin-encoding lentivirus to reduce the dystrophic pathology in late-stage
dmd mice. The histopathological and physiological-functional regeneration activities of the lentiviralmicro-
Dystrophin gene therapy methods were compared, along with changes in temporal Dystrophin
expression and their functionality, toxicity, and gene expression level.

Results:
Here, we showed that the micro-dystrophin transgene transfers intramuscularly and intraperitoneally
in late-stage dmd-mdx-4cv mice restored dystrophin expression in the skeletal and cardiac
muscle (p <0.001). Furthermore, motor performance analysis, including hanging and tracking tests,
improved statistically significantly after the treatment (p <0.05).

Conclusion:
Consequently, this study suggests that patients in the late stages of muscular dystrophy
can benefit from lentiviral micro-dystrophin gene therapies to present an improvement in dystrophic
muscle pathology.
Molecular Modeling of some 1,3,4-Oxadiazole Derivatives as EGFR Inhibitors for the Treatment of Cancer
Authors: Patil Shital M. , Bhandari Shashikant V. , Patil Varsha A. , Randive Vrushali , Mahadik Indrani 
Published: April 2023

Background:
Cancer is a group of illnesses characterised by the impartial increase and unfolding
of somatic cells. A variety of natural compounds, such as curcumin, zingiberine and their composites,
and synthetic organic derivatives of imidazole, benzothiazole, oxadiazole, quinazoline etc., have been
developed as anticancer agents. But many of these show various side effects, drug resilience, and toxicity
to the normal cells of the host body. Therefore, there is still demand to develop new synthetic derivatives
to improve their pharmacological profile.

Objective:
The Aim of this study is to understand the binding mode and to check the drug likeliness of
numerous 1,3,4-oxadiazole derivatives as EGFR inhibitors for the treatment of cancer. The objective of
the study is to screen newly designed derivatives of 1,3,4-oxadiazole using molecular docking and
ADMET studies as EGFR inhibitors.

Methods:
In silico docking studies were performed using AutoDockVina software, and compounds were
further studied for ADME and toxicity using SwissADME and pkCSM software, respectively.

Results:
Considering the docking results, pharmacokinetic behaviour and toxicity profile, eight derivatives
(derivatives 2,3,4,5,10,13,16, and 17) showed potential as EGFR inhibitors.

Conclusion:
Compounds 2 and 3 showed the highest binding affinity in the pocket of EGFR and also
displayed a better pharmacokinetic profile. Therefore, these derivatives can be used in the management of
cancer and can be taken further for wet-lab studies.
Isolation and Identification of Saccharomyces boulardii as a Probiotic Yeast and Investigation of its In vitro and In vivo Beneficial Applications
Authors: Helmy Eman A. , Abdel-Fadeel Reda H. , El-Husseiny  Helal Eman , Yosri Mohammed 
Published: April 2023

Background:
Yeasts are becoming increasingly popular as possible new resources to
improve the nutritional value of food qualities like flavor, color, and vitamin content, as well as
tools for preventing food spoiling due to their anti-microbial capabilities.

Objective:
This study aims to test the role of isolated Saccharomyces boulardii as a promising probiotic
as well as Jerusalem artichoke as a prebiotic in a feeding animal model to test their possible
functions in regulating lipid profile and bacterial count in the faeces of tested animals.

Methods:
For the isolation of yeast cultures on YM agar medium, five milk samples were randomly
obtained from the Faculty of Agriculture at Al-Azhar University in Cairo, Egypt. S. boulardii was
morphologically, physiologically, and molecularly identified and deposited in the gene bank; additionally,
identification was confirmed using the BIOLOG system. For 30 days, researchers compared
the roles of S. boulardii as a probiotic and Jerusalem artichoke as a prebiotic in controlling
serum lipid profile and faeces microbial count in the tested animal groups.

Results:
The acid and bile tolerance tests illustrate the promising ability of S. boulardii. Additionally,
S. boulardii could survive on simulated stomach and intestinal fluids. An in vivo feeding test
showed that rats fed S. boulardii grew and gained weight faster. Furthermore, eating S. boulardii
causes a marked increase in HDL levels and a marked drop in LDL, cholesterol, and triglycerides.
Rats that consumed Jerusalem artichokes and a probiotic strain had significantly lower numbers of
coliforms and Staphylococcus sp. in their stools.

Conclusion:
These results revealed the possible beneficial role of S. boulardii in overcoming hyperlipidemia,
which should be applied on a large scale after verification of outcomes.
The Perception and Usage of Fake Braces: Twitter Content Analysis
Authors: Samman Meyassara , Bahanan Lina 
Published: April 2023

Introduction:
Financial concerns deter many people from seeking orthodontic care. Fake braces replicate the real orthodontic braces but are not functional and are priced at a lower cost. These products are available through illegal and unregistered merchants, who use social media for advertising their products. This study aimed to analyze Arabic Twitter posts related to fake braces.

Methods:
Tweets were collected from Twitter API v2 using Python. The search was limited to the Arabic language, using keywords such as “fake braces” and “fashion braces.” Tweets were collected in the period from January 1, 2020, until July 31, 2021. Both sentiment and thematic analyses were performed.

Results:
788 tweets met the inclusion criteria, 660 of which were advertisements. The rest of the tweets (n = 128) were categorized into four main themes: rejecting the idea of fake braces (49.2%), having the intention to buy fake braces (32.0%), using/used fake braces (14.1%), and accepting the idea of fake braces (4.7%). Sentiment analysis showed that more than half of the tweets (55.5%) were negative and mainly involved sarcastic language (65.8%).

Conclusion:
Although many of the tweets were against the use of fake braces, a sizable number of advertisement tweets promoted fake braces. Efforts should be made to educate the public about the risks of using fake braces and to emphasize the importance of seeking a licensed orthodontist for treatment. In addition, health authorities must establish laws and policies and enhance surveillance to control social media content advertising these harmful products.
The Etiopathogenesis and Genetic Factors in Idiopathic Inflammatory Myopathies: A Review Article
Authors: Lugo-Zamudio Gustavo-Esteban , Barbosa-Cobos Rosa-Elda , Maya-Piña Lucía-Verónica , Delgado-Ochoa Dolores , López-Mayorga María-Mercedes , Arenas-Silva Ivonne , Arellano-Álvarez Diana-Sarai 
Published: April 2023

Introduction:
Idiopathic inflammatory myopathies (IIM) are a group of heterogeneous systemic autoimmune diseases characterized by muscle inflammation from unknown causes resulting in chronic weakness. Recent studies have shown the role of the cellular immune response affecting muscle fibers in polymyositis (PM), inclusion body myositis, and to a lesser extent, dermatomyositis (DM), wherein humoral immunity is more involved. The value of genetic factors of the class II major histocompatibility complex (MHC II) has also been highlighted. In studies of murine models, the presence of HLA-DR3 favors a higher risk of developing inflammatory muscle disease, including PM and juvenile DM. In recent years, few studies have provided timely information regarding this, thus the researchers initially proposed a review of existing literature to broaden the context regarding what was described and to visualize proposals that may enhance the understanding of this group of inflammatory pathologies.

Methods:
The design, implementation, analysis, and reporting of this study were followed according to the search with MeSH terms (Autoimmune myopathy, Inflammatory myopathies, Idiopathic inflammatory myopathies AND Major histocompatibility complex and genetics). We analyzed 12 articles for this review article.

Conclusion:
In the etiopathogenesis of IIM, both humoral and cellular immunity are observed, considering the presence of a trigger that causes the immune response. As for the immunogenetics, this review highlights what has been reported in Chinese and Mexican populations, where HLADRB1*09:01 is related to the presence of DM, and is observed as the first variant identified in various populations. This increases interest in this allele in the particular case to study DM and strengthens research that proposes the study of IIM independently for each nosological entity.
Control of Bacterial Wilt (Ralstonia solanacearum) and Reduction of Ginger Yield Loss through Integrated Management Methods in Southwestern Ethiopia
Authors: Benti Eyob Aysanew 
Published: April 2023

Background:
Bacterial wilt incited by Ralstonia solanacearum is the most important disease affecting ginger production in southwestern Ethiopia. The unavailability of disease-free planting materials, resistant cultivars, and effective chemical compounds are the key constraints in managing the disease.

Objective:
The study was initiated to determine the effect of integrated management methods on bacterial wilt disease and yield loss of ginger through combining hot water, bio-fumigation, soil-solarization and chemical pesticides.

Methods:
A total of seven treatment combinations comprising hot water, bio-fumigation, soil-solarization, Mancozeb, and bleaching powder were tested in a randomized complete block design in three replications. Data on disease incidence, growth, yield, and yield components were recorded from randomly selected plants.

Results:
The use of Mancozeb for seed socking and soil drenching combined with bio-fumigation and soil-solarization reduced the incidence of bacterial wilt by 63.3% and enhanced the rhizome yield by 66.8%. Rhizome and soil treatment using bleaching powder along with soil bio-fumigation also reduced the disease incidence by 38.9% and increased ginger yield by 61.5%. It also provided the highest (6678.7%) marginal rate of return of any treatment combination tested in the experiment. Disease incidence was highly significantly and inversely (r= -0.98**) correlated with rhizome yield. The regression slope estimated that 83.4% of ginger yield loss was associated with bacterial wilt disease.

Conclusion:
A combined application of Mancozeb, bio-fumigation and soil-solarization can be used to control ginger bacterial wilt. Alternatively, bleaching powder for rhizome and soil treatment in conjunction with bio-fumigation can be employed as an integrated management system against the disease.
Vogt-Koyanagi-Harada-like Syndrome and Electronegative Electroretinogram after Nivolumab Therapy for Metastatic Cutaneous Melanoma: A Case Report
Authors: Pole Cameron , Su Erin , Kesavamoorthy Niranjana , Gokoffski Kimberly , Sanfilippo Christian , Ameri Hossein 
Published: April 2023

Background:
Vogt-Koyanagi-Harada-like (VKH) syndrome or electronegative electroretinograms (ERG) have both been described before or after immunotherapy for metastatic cutaneous melanoma, but they have not been described occurring together.

Objective:
The objective of this study is to describe a case of VKH-like syndrome occurring simultaneously with an electronegative ERG after nivolumab therapy for metastatic cutaneous melanoma.

Methods:
Case report of a patient with ocular findings after nivolumab therapy for metastatic melanoma was reported. Multimodal imaging, including color fundus photography, optical coherence tomography (OCT), and both full-field and multifocal ERGs were used to describe the findings. Literature review was conducted with PubMed.

Results:
We reported a case of a patient with nivolumab-treated melanoma presenting with presumed VKH-like syndrome with panuveitis, choroidal depigmentation, and cutaneous vitiligo, as well as melanoma-associated retinopathy (MAR)-like electronegative ERG findings. Nivolumab was stopped and corticosteroid therapy was initiated. Although the patient’s visual acuity remained severely limited, her inflammation resolved, and the areas of choroidal depigmentation slowly decreased over years of subsequent follow-up.

Conclusion:
Vision loss accompanied by simultaneous VKH-like findings with choroidal vitiligo and a MAR-like electronegative ERG may develop after nivolumab therapy. The uveitic and vitiligo may improve with immunosuppressive therapy, but the vision loss and ERG findings may persist.
Transcutaneous Radiofrequency-mediated Meibomian Gland Expression is an Effective Treatment for Dry Eye: A Prospective Cohort Trial
Authors: Paul Sean , Cohen Alex , Parsa Kami , Jaccoma Edward , Burrell Kim , Carruthers Jean 
Published: April 2023

Purpose:
Meibomian gland dysfunction disrupts tear film homeostasis and results in dry eye syndrome. The objective of this study is to determine whether transcutaneous radiofrequency (RF)-assisted meibomian gland expression, using the Envision platform and Forma-I handpiece is an effective treatment for dry eye syndrome.

Methods:
A multicenter prospective cohort study of patients with dry eye undergoing RF-assisted meibomian gland expression was completed from October 2019 to June 2022. The study was designed with multiple primary endpoints. These were defined as the change in Standard Patient Evaluation of Eye Dryness (SPEED) score, changes in Ocular Surface Disease Index (OSDI), Tear Breakup Time (TBUT), Corneal Fluorescence Score (CFS), and Meibomian Gland Score (MGS) at 1, 3, and 6 months after treatment. Secondary endpoints were measurements of patients' subjective improvement and subjective satisfaction with the treatment.

Results:
A total number of 47 patients were enrolled in the study at three separate institutions by three ophthalmologists (oculoplastics, refractive, and general). However, not all subjects had complete data on all observations at all time points following treatment. When a data point was missing, the entire patient’s outcome for that data set was excluded from the analysis. There was a significant improvement in SPEED score from baseline, 15.7 vs 11.4 at 1 month, 9.1 at 3 months, and 9.6 at 6 months (p<0.05). There was also a statistically significant improvement noted for OSDI at all time points measured, 34.5 at baseline vs 25.2 at 1 month, 21.2 at 3 months, and 23.6 at 6 months (p<0.5). CFS was significantly reduced in each eye at all time points following treatment as well, with 80% of eyes responding. TBUT similarly improved after treatment in each eye with an average of 6.3s at 1 month, 7.1s at 3 months, and 7.1s after treatment at 6 months vs 2.8s at baseline. The MGS also showed marked improvement across all time points, 5.6 at baseline vs 19.9 at 1 month, 24.7 at 3 months, and 22.9 at 6 months. Patients noted subjective improvement, with a lack of pain and discomfort from the treatment.

Conclusion:
This pilot study demonstrates that RF treatment with the Forma-I handpiece along with meibomian gland expression is an effective means to reduce the signs and symptoms of dry eye disease. The data support the conclusion that the treatment is safe and effective, lasting at least 6 months in most patients.
Relationship between Health-promoting practices and the Health-related Quality of Life among Saudi University Students after the COVID-19 Pandemic Lockdown: Cross-sectional Study
Authors: Abuadas Fuad H. 
Published: April 2023

Introduction:
Promoting healthy living activities among university students is acknowledged as an essential component in health maintenance and improvement.

Objectives:
This study aims to determine the extent to which Saudi undergraduate students are involved in health-promoting practices after the COVID-19 pandemic lockdown. It also investigates how the health-related quality of life (QOL) could be predicted from sociodemographics and health-promoting practices.

Methods:
This study adopted a descriptive correlational design and a convenience sampling method to recruit 542 Saudi students from 5 governmental universities in multiple Saudi regions between October and December 2022. University students were asked to complete an online survey consisting of sociodemographics, the health-promoting lifestyle profile II (52 Items, 4 points Likert scale), and the WHOQOLscale (26 Items, 5 points Likert scale).

Results:
In the health-promoting subscales, participants ranked best in spiritual growth (M = 2.79) but worst in physical activity (M = 1.82). Regarding the QOL subscales, participants scored the highest in social relationships (M = 14.32) and the lowest in psychological health (M = 11.36). Multiple linear regression revealed that 19% of the variance in the QOL was explained mainly by health-promoting variables, in which physical activity (β = .20, p < .001) was the strongest predictor. In addition, family income (β = -.14, p < .01), nutrition (β = .12, p < .05), stress management (β = .11, p < .05), and spiritual growth (β = .14, p < .05) were also significant predictors of QOL.

Conclusion:
Physical activity, nutrition, spiritual growth, and stress management have been found helpful in improving the QOL of Saudi students. Therefore, universities should consider planning curricula and establishing activities that encourage involvement in health-promoting activities.
Comparing Eye-tracking System Effectiveness in Field and Driving Simulator Studies
Authors: Calvi Alessandro , D’Amico Fabrizio , Vennarucci Andrea 
Published: April 2023

Background:
Several studies have been conducted by combining the benefits of eye-tracking systems with driving simulators to simultaneously investigate driving behavior and the potential source of distraction. However, little effort has been made in terms of eye-tracking validation in the driving simulator environment.

Objective:
The overall aim of this study is to validate an eye-tracking system within the context of a driving simulation environment by considering a specific urban context application.

Methods:
A field survey and a driving simulation experiment were conducted for a case study in Rome, Italy. The selected real road sections and events were reproduced on the driving simulator system. An eye-tracking system was used to record eye movements both on a real vehicle and the simulator. The eye movements of 14 participants in the field survey and 18 participants in the driving simulation tests, as well as their driving performances, were collected while approaching an urban intersection in relation to two specific road events: i) the presence of a speed limit sign and ii) the presence of a crossing pedestrian.

Results:
Eye tracker parameters and driving performances were compared between the real driving tests and driving simulator experiments in order to validate the eye-tracking system. It was validated for both events in terms of duration and distance of the eye fixation.

Conclusion:
The results demonstrated that an eye-tracking system is an effective tool for studies and applications in a virtual reality environment.
Seroprevalence of SARS-Cov-2 Virus Infection In Kermanshah, Iran: A Population-based Cross-Sectional Study
Authors: Shadmani Fatemeh Khosravi , Najafi Farid , Mansori Kamyar , Moradi Mahmoud Reza , Rezaei Mansor , Rezvani Nayebali , Darbandi Mitra , Yoosefi Moein , Laghei Zahra , Bozorgomid Arezoo , Salahshoor Pegah , Shakiba Ebrahim 
Published: April 2023

Background:
The aim of this study was to estimate the seroprevalence of SARS-CoV-2 infection in a general population from Kermanshah province, Iran.

Methods:
The present study was a population-based cross-sectional design conducted in Kermanshah province in 2020. Sampling was performed in a multi-stage process, and 1967 participants were considered, and also 174 interviewers were assigned to collect data online. Then, 5 mL of blood sample was taken from every participant. The blood samples were centrifuged with the ELISA method to detect SARS-CoV-2-specific IgG and IgM antibodies in serum samples. Seropositive prevalence was adjusted by means of survey analysis. Case fatality rate (CFR) and infection fatality rate (IFR) were estimated.

Results:
A total of 1967 people from 14 cities of the province participated in the study. The mean age of participants was 35.7±16.9, and 50.4% were female. The lowest and highest seroprevalence was found in the cities of Paveh (2.3% [0.3- 4.2]) and Harsin (61.6% [54.7–68.5]), respectively. The CFR and IFR in men and women were 3.4 vs. 3.3 and 0.1 vs. 0.3. The aged 60 years or older had the highest CFR and IFR with 11.2 and 3.7%, respectively.

Conclusion:
The prevalence of SARS-CoV-2 infection and IFR among the general population of Kermanshah province was 18.3 and 0.3%, respectively. The results of this study can assist the policymaker in assessing risk factors, and transmission dynamics of SARS-CoV-2 in a population and implementing preventive and control interventions.
Self-care Behaviors and Lipid Profiles among an Ethnic Minority Adult Population in Thai Rural Communities
Authors: Mafu Chitipat , Seangpraw Katekaew , Ong-Artborirak Parichat , Boonyathee Sorawit , Tonchoy Prakasit , Kantow Supakan , Auttama Nisarat , Choowanthanapakorn Monchanok , Winaiprasert Prakaipetch 
Published: April 2023

Background:
Health perceptions and increased self-care behaviors can effectively prevent and control many diseases and improve one’s health. This study aimed to investigate the association of self-care behaviors with lipid profiles and creatinine among an ethnic minority adult population in Thai rural communities.

Methods:
A cross-sectional study was performed from January to April 2021 among ethnic minorities from hill tribe communities of Phayao Province, Northern Thailand. A total of 252 adults ages 20 and older were recruited from various ethnic groups, including Indigenous (34.5%), Hmong (25.4%), and Mien (40.1%) peoples. The data were obtained from face-to-face interviews using questionnaires and from blood samples.

Results:
Multiple linear regression revealed that self-care behavior score was associated with ethnicity, age, education, and self-efficacy score (p < 0.05). Total cholesterol level was associated with ethnicity, gender, financial status, drinking alcohol, smoking, and underlying disease (p < 0.05). Low-density lipoprotein cholesterol level was associated with gender, financial status, and drinking alcohol (p < 0.05). Creatinine level was predicted by ethnicity, gender, age, smoking, and self-care behaviors (p < 0.05). Alcohol consumption was found to be a predictor of body mass index and blood pressure (p < 0.05).

Conclusion:
Health promotion programs for specific populations should emphasize public health benefits (e.g., reducing risk factors of non-communicable diseases (NCDs), increasing health awareness and health beliefs for better outcome expectations, and enhancing the ability to perform self-health management routinely) in accordance with the local context of the studied population.