bluuebunny/crossref_metadata_2025_split huggingface dataset:
Reduced digestion of circulating genomic DNA in systemic sclerosis patients with the DNASE1L3 R206C variant
Authors: Skaug Brian , Guo Xinjian , Li Yuanteng Jeff , Charles Julio , Pham Kay T , Couturier Jacob , Lewis Dorothy E , Bracaglia Claudia , Caiello Ivan , Mayes Maureen D , Assassi Shervin 
Published: January 2023

Abstract

Objectives
Polymorphism in a coding region of deoxyribonuclease I-like III (DNASE1L3), causing amino acid substitution of Arg-206 to Cys (R206C), is a robustly replicated heritable risk factor for SSc and other autoimmune diseases. DNASE1L3 is secreted into the circulation, where it can digest genomic DNA (gDNA) in apoptosis-derived membrane vesicles (AdMVs). We sought to determine the impact of DNASE1L3 R206C on digestion of circulating gDNA in SSc patients and healthy controls (HCs).

Methods
The ability of DNASE1L3 to digest AdMV-associated gDNA was tested in vitro. The effect of R206C substitution on extracellular secretion of DNASE1L3 was determined using a transfected cell line and primary monocyte-derived dendritic cells from SSc patients. Plasma samples from SSc patients and HCs with DNASE1L3 R206C or R206 wild type were compared for their ability to digest AdMV-associated gDNA. The digestion status of endogenous gDNA in plasma samples from 123 SSc patients and 74 HCs was determined by measuring the proportion of relatively long to short gDNA fragments.

Results
The unique ability of DNASE1L3 to digest AdMV-associated gDNA was confirmed. Extracellular secretion of DNASE1L3 R206C was impaired. Plasma from individuals with DNASE1L3 R206C had reduced ability to digest AdMV-associated gDNA. The ratio of long: short gDNA fragments was increased in plasma from SSc patients with DNASE1L3 R206C, and this ratio correlated inversely with DNase activity.

Conclusion
Our results confirm that circulating gDNA is a physiological DNASE1L3 substrate and show that its digestion is reduced in SSc patients with the DNASE1L3 R206C variant.
Factors Associated with Chinese Migrant Children’s Social Engagement during the COVID-19 Pandemic: A Case Study in Guangzhou City, China
Authors: Huang Dan , Lin Wenyi , Luo Yingting 
Published: January 2023

AbstractThis study aims to examine whether social work services can improve migrant children’s social engagement during the COVID-19 pandemic in urban China. Valid questionnaires of 416 migrant children in Guangzhou were obtained as the samples. Two regression models were employed to analyze the factors associated with social engagement. The results show that during the pandemic, migrant children with higher self-efficacy, higher community social capital, and better social inclusion tended to have higher social engagement. Among the three dimensions of social capital, only community social capital has a significant positive effect on migrant children’s social engagement. The degree of social inclusion of migrant children affects their social engagement. Social work services are beneficial to promoting the social engagement of migrant children. The research has implications for the practice of child social workers. First, it is important to cultivate migrant children’s self-efficacy to promote social engagement. Second, social workers should build the social capital of the communities where migrant children live to promote the social engagement of this group. Third, social workers should strengthen support for migrant children’s learning during the COVID-19 pandemic.
Using precision tools to manage and evaluate the effects of mineral and protein/energy supplements fed to grazing beef heifers
Authors: McCarthy Kacie L , Underdahl Sarah R , Undi Michael , Dahlen Carl R 
Published: January 2023

Abstract
Our objectives were to develop a Mobile Cow Command Center (MCCC) capable of precision monitoring of grazing heifers to 1) examine the relationship between supplement intake on concentrations of liver mineral and blood metabolites and 2) examine activity, reproductive, and health behavior. Yearling crossbred Angus heifers (N = 60; initial BW = 400.4 ± 6.2 kg) were fitted with radio frequency identification ear tags that allowed access to electronic feeders (SmartFeed system; C-Lock Inc., Rapid City, SD), and with activity monitoring tags (CowManager B.V., the Netherlands) that monitored reproductive, feeding, and health-associated behaviors. Heifers were assigned randomly to one of three treatments for a 57-day monitoring period: 1) no supplement (CON; N = 20), 2) free choice mineral (MIN; Purina Wind and Rain Storm [Land O’Lakes, Inc.], N = 20), or 3) free choice energy and mineral supplement (NRG; Purina Accuration Range Supplement 33 with added MIN [Land O’Lakes, Inc.], N = 20). Consecutive day body weights, blood, and liver biopsies were collected at pasture turnout and final day of monitoring. By design, mineral intake was greatest in MIN heifers (49 ± 37 g/d) and energy supplement intake was greatest in NRG heifers (1,257 ± 37 g/d). Final BW and ADG were similar among treatments (P &gt; 0.42). Concentrations of glucose on day 57 were greater (P = 0.01) in NRG compared with CON and MIN heifers. Liver concentrations of Se and Fe on day 57 were greater (P &lt; 0.05) in NRG heifers than CON, with MIN being intermediate. Activity tags reported NRG heifers spent less time eating (P &lt; 0.0001) and more time (P &lt; 0.0001) being “highly active” than MIN with CON heifers being intermediate. Data retrieved from activity tags identified 16 of 28 pregnant heifers exhibiting some type of estrus-associated behavior even after confirmation of established pregnancy. The activity monitoring system triggered a total of 146 health alerts from 34 of the 60 heifers monitored, but only 3 heifers of the heifers initiating an electronic health alert needed clinical treatment. However, animal care staff identified nine additional heifers that required treatment for which no electronic health alert was generated. The electronic feeders successfully controlled intake of individual heifers managed in groups pastures; however, the activity monitoring system misrepresented estrus and health events.
Using heat plumes to simulate post-fire effects on cambial viability and hydraulic performance in Sequoia sempervirens stems
Authors: Salladay Ryan A , Pittermann Jarmila 
Published: January 2023

Abstract
Injury to the xylem and vascular cambium is proposed to explain mortality following low severity fires. These tissues have been assessed independently, but the relative significance of the xylem and cambium is still uncertain. The goal of this study is to evaluate the xylem dysfunction hypothesis and cambium necrosis hypothesis simultaneously. The hot dry conditions of a low severity fire were simulated in a drying oven, exposing Sequoia sempervirens (Lamb. ex D. Don) shoots to 70 and 100 °C for 6–60 min. Cambial viability was measured with Neutral Red stain and water transport capacity was assessed by calculating the loss of hydraulic conductivity. Vulnerability curves were also constructed to determine susceptibility to drought-induced embolism following heat exposure. The vascular cambium died completely at 100 °C after only 6 min of heat exposure, while cells remained viable at 70 °C temperatures for up to 15 min. Sixty minutes of exposure to 70 °C reduced stem hydraulic conductivity by 40%, while 45 min at 100 °C caused complete loss of conductivity. The heat treatments dropped hydraulic conductivity irrecoverably but did not significantly impact post-fire vulnerability to embolism. Overall, the damaging effects of high temperature occurred more rapidly in the vascular cambium than xylem following heat exposure. Importantly, the xylem remained functional until the most extreme treatments, long after the vascular cambium had died. Our results suggest that the viability of the vascular cambium may be more critical to post-fire survival than xylem function in S. sempervirens. Given the complexity of fire, we recommend ground-truthing the cambial and xylem post-fire response on a diverse range of species.
Ring- and diffuse-porous tree species from a cold temperate forest diverge in stem hydraulic traits, leaf photosynthetic traits, growth rate and altitudinal distribution
Authors: Yin Xiao-Han , Hao Guang-You , Sterck Frank 
Published: January 2023

Abstract
In cold and humid temperate forests, low temperature, late frost and frequent freeze–thaw cycles are the main factors limiting tree growth and survival. Ring- and diffuse-porous tree species differing in xylem anatomy coexist in these forests, but their divergent adaptations to these factors have been poorly explored. To fill this knowledge gap, we compared four ring-porous and four diffuse-porous tree species from the same temperate forest in Northeast China by quantifying their leaf and stem functional traits, their stem growth rates using tree ring analysis and their resistance to cold represented by upper altitude species distribution borders from survey data. We found that the ring-porous trees were characterized by traits related to more rapid water transport, carbon gain and stem growth rates than those of the diffuse-porous species. Compared with the diffuse-porous species, the ring-porous species had a significantly higher shoot hydraulic conductance (Ks-shoot, 0.52 vs 1.03 kg m−1 s−1 MPa−1), leaf photosynthetic rate (An, 11.28 vs 15.83 μmol m−2 s−1), relative basal area increment (BAIr, 2.28 vs 0.72 cm year−1) and stem biomass increment (M, 0.34 vs 0.09 kg year−1 m−1). However, the observed upper elevational distribution limit of the diffuse-porous species was higher than that of the ring-porous species and was associated with higher values of conservative traits, such as longer leaf life span (R2 = 0.52). Correspondingly, BAIr and M showed significant positive correlations with acquisitive traits such as Ks-shoot (R2 = 0.77) and leaf photosynthetic rate (R2 = 0.73) across the eight species, with the ring-porous species occurring at the fast-acquisitive side of the spectrum and the diffuse-porous species located on the opposite side. The observed contrasts in functional traits between the two species groups improved our understanding of their differences in terms of growth strategies and adaptive capabilities in the cold, humid temperate forests.
Red pigments in autumn leaves of Norway maple do not offer significant photoprotection but coincide with stress symptoms
Authors: Mattila Heta , Tyystjärvi Esa 
Published: January 2023

Abstract
The reasons behind autumn colors, a striking manifestation of anthocyanin synthesis in plants, are poorly understood. Usually, not all leaves of an anthocyanic plant turn red or only a part of the leaf blade turns red. In the present study, we compared green, red and yellow sections of senescing Norway maple leaves, asking if red pigments offer photoprotection, and if so, whether the protection benefits the senescing tree. Green and senescing maple leaves were illuminated with strong white, green or red light in the absence or presence of lincomycin which blocks photosystem II (PSII) repair. Irrespective of the presence of anthocyanins, senescing leaves showed weaker capacity to repair PSII than green leaves. Furthermore, the rate of photoinhibition of PSII did not significantly differ between red and yellow sections of senescing maple leaves. We also followed pigment contents and photosynthetic reactions in individual leaves, from the end of summer until abscission of the leaf. In maple, red pigments accumulated only during late senescence, but light reactions stayed active until most of the chlorophyll had been degraded. PSII activity was found to be lower and non-photochemical quenching higher in red leaf sections, compared with yellow sections of senescing leaves. Red leaf sections were also thicker. We suggest that the primary function of anthocyanin synthesis is not to protect senescing leaves from excess light but to dispose of carbohydrates. This would relieve photosynthetic control, allowing the light reactions to produce energy for nutrient translocation at the last phase of autumn senescence when carbon skeletons are no longer needed.
Ability of clock drawing errors on Mini-Cog test to predict development of delirium after major urological cancer surgery
Authors: Yajima Shugo , Nakanishi Yasukazu , Matsumoto Shunya , Okubo Naoya , Tanabe Kenji , Kataoka Madoka , Masuda Hitoshi 
Published: January 2023

Abstract

Background
This study aimed to evaluate the impact of the clock drawing test (CDT) on postoperative delirium and compare the abilities of the mini-cognitive (Mini-Cog) test and the CDT for predicting postoperative delirium after major urological cancer surgery.

Materials and methods
In this single-center retrospective observational study, we collected the medical records of patients who underwent major urologic cancer surgery and preoperative cognitive screening based on the Mini-Cog test consisting of the CDT and the 3-word recall task at our department in 2020–2021 (n = 387). Univariate and multivariate logistic regression analyses were used to identify the clinical risk factors for postoperative delirium. We also compared the ability of the CDT alone and the Mini-Cog test consisting of the CDT and 3-word recall task to predict postoperative delirium.

Results
A total of 117 patients (30%) had abnormal CDT results. Postoperative delirium occurred in 29 patients (7%). On multivariate analysis, American Society of Anesthesiologists physical status ≥3 (odds ratio [OR], 5.0; p = 0.01), abnormal CDT (OR, 4.8; p < 0.001), preoperative benzodiazepine use (OR, 4.9; p < 0.001), and operative time ≥237 minutes (OR, 3.0; p = 0.01) were independent risk factors for postoperative delirium. The area under the curve for predicting postoperative delirium was 0.709 for CDT alone and 0.743 for the Mini-Cog test. No significant intergroup difference was observed (p = 0.43).

Conclusions
The CDT served as a formal but simple tool with adequate predictive power to identify the risk of postoperative delirium among patients undergoing major urological cancer surgery. Effective screening using the CDT might help provide optimal urological care for older patients.
Adipose Tissue Transfer in Dynamic Definition Liposculpture Part II. The Lower Limb: Gastrocnemius, Vastus Medialis, Vastus Lateralis, and Rectus Femoris Muscles
Authors: Hoyos Alfredo E. , Stefanelli Matt , Perez Mauricio E. , Padilla Mauricio , Dominguez-Millan Rodrigo 
Published: January 2023

Background:
One big challenge of body contouring surgery is the liposculpture of the lower limbs, probably because of the imperative symmetry and the risk of contour irregularities. We are reporting our experience in fat grafting of the thighs and calves for men and women undergoing dynamic definition liposculpture.

Methods:
We did cadaveric dissections of the vastus lateralis, vastus medialis, rectus femoris, and gastrocnemius muscles and identified each of their primary pedicles. We also performed fat grafting of these muscles in the contralateral virgin cadaveric specimen. We searched our records for patients who underwent fat grafting of the lower extremity in addition to dynamic definition liposculpture, from January 2016 to May 2022 at a single center in Bogotá, Colombia.

Results:
Seventy-three consecutive patients met the inclusion criteria (26 men and 47 women). We grafted 102 gastrocnemius muscles, 86 vastus medialis muscles, 98 vastus lateralis muscles, and 22 rectus femoris muscles. Mean age was 34 and 41 years for men and women, respectively. Range of the fat graft volume was 50–200 mL. No complications were recorded related to fat grafting. Almost all patients were satisfied with the procedure (89%). Follow-up period ranged from 2 to 36 months.

Conclusions:
Fat grafting of the lower limb muscles should be considered an alternative operative technique to enhance volume and athletic appearance of this body segment. Based on cadaveric dissections and clinical evidence, our technique is considered reliable and reproducible with remarkable outcomes and a very low complication rate.
Systematic Review of Online Interventions to Reduce Perinatal Mood and Anxiety Disorders in Underserved Populations
Authors: Canfield Shannon M. , Canada Kelli E. 
Published: January 2023

Background:
Online health interventions increase access to care, are acceptable to end users and effective for treating mental and physical health disorders. However, less is known about interventions to prevent and treat perinatal mood and anxiety disorders (PMADs). This review synthesizes existing research on PMAD prevention and treatment by exploring the treatment modalities and efficacy of online interventions and examining the inclusion of underserved populations in PMAD research.

Methods:
Using PRISMA guidelines, authors conducted a systematic review of peer-reviewed literature published between 2008 and 2018 on online interventions aimed to prevent or treat PMADs. The authors also assessed quality. Eligible articles included perinatal women participating in preventive studies or those aimed to reduce symptoms of PMADs and utilized a Web-based, Internet, or smartphone technology requiring an online component. This study excluded telephone-based interventions that required one-on-one conversations or individualized, text-based responses without a Web-based aspect.

Results:
The initial search yielded 511 articles, and the final analysis included 23 articles reporting on 22 interventions. Most studies used an experimental design. However, no study achieved an excellent or good quality rating. Psychoeducation and cognitive-behavioral therapies (CBTs) were most common. Several interventions using CBT strategies significantly decreased depression or anxiety. Four studies recruited and enrolled mainly people identifying as low-income or of a racial or ethnic minority group. Attrition was generally high across studies.

Discussion:
More research using rigorous study designs to test PMAD interventions across all perinatal times is needed. Future research needs to engage diverse populations purposefully.
Positive hepatitis B core antibody is associated with advanced fibrosis and mortality in nonalcoholic fatty liver disease
Authors: Zhang Pengyan , Liu Zhenqiu , Fan Hong , Shi Tingting , Han Xinyu , Suo Chen , Chen Xingdong , Zhang Tiejun 
Published: January 2023

Objective
Concomitant hepatitis B virus infection and nonalcoholic fatty liver disease (NAFLD) are relatively common, while little is known about the impact of anti-hepatitis B core antibody (anti-HBc) on NAFLD individuals. We aimed to investigate the association of positive anti-HBc with advanced fibrosis and mortality in NAFLD.

Methods
We analyzed data from 3268 NAFLD participants who underwent abdominal ultrasonography during the Third National Health and Nutrition Examination Survey (NHANES III). The fibrosis 4 index (FIB-4) score >2.67, NAFLD fibrosis score >0.676, or aspartate aminotransferase to platelet ratio index >1.5 were defined as advanced fibrosis. All-cause and cause-specific mortality were obtained from the NHANES III-linked follow-up file through 31 December 2015.

Results
A total of 242 (7.4%) patients had positive anti-HBc. Patients with positive anti-HBc had a higher percentage of advanced fibrosis than those with negative anti-HBc (12.2% vs. 5.8%). Positive anti-HBc was significantly associated with advanced fibrosis [adjusted odds ratio = 1.69, 95% confidence interval (CI), 1.05–2.72]. During a median follow-up of 22 years, the cumulative all-cause and cancer-related mortalities were higher in participants with positive anti-HBc than in their counterparts (log-rank test P < 0.001). When demographic and metabolic risk factors were considered, NAFLD cases with positive anti-HBc had a significantly higher cancer-related mortality (adjusted hazard ratio = 1.54, 95% CI, 1.05–2.25).

Conclusion
Our findings suggested that NAFLD cases with positive anti-HBc had higher risks for liver fibrosis and long-term mortality, justifying the medical importance of testing anti-HBc in NAFLD patients.
Development and validation of the Adult cirrhosiS Knowledge Questionnaire
Authors: Leung Hak Keith , Mahadeva Sanjiv , Rajaram Ruveena Bahavani , Lai Pauline Siew Mei 
Published: January 2023

Background
Assessing a patient’s knowledge regarding liver cirrhosis is important to improve patient outcomes. This study aimed to develop and validate the Adult cirrhosiS Knowledge Questionnaire (ASK-Q) to assess patients’ knowledge regarding liver cirrhosis from multiple aspects.

Methods
A 24-item ASK-Q with four domains: self-understanding (5 items), aetiology (5 items), complications (5 items) and management (9 items) of liver cirrhosis was developed based on literature review and expert panel input. It was then piloted in five English-speaking patients with liver cirrhosis. These patients commented that the font size was too small. Hence, the font was enlarged and the final version of the ASK-Q was administered to English-speaking patients with liver cirrhosis, aged ≥18 years, with or without decompensation, at a tertiary hospital, from September 2020 to November 2021, at baseline and fortnight later. Patients with encephalopathy were excluded.

Results
120/135 patients agreed to participate (response rate = 88.9%). The overall median score was 59.1 (45.6–68.2). A total of 7/22 (31.8%) items were “easy”, 14/22 (63.6%) items were “moderately easy” and 1/22 (4.5%) items were “difficult”. Exploratory factor analysis extracted nine factors, and two items were omitted. The ASK-Q was able to discriminate the knowledge level of patients with and without tertiary education [59.1 (50.0–72.7) vs. 54.5 (36.4–63.6); P < 0.05]. The overall Kuder-Richardson coefficient was 0.760, indicating adequate internal consistency. At retest, 77/120 patients participated (response rate = 64.2%) and 15/22 items were not statistically significant, indicating adequate reliability.

Conclusions
The ASK-Q was found to be a valid and reliable questionnaire for evaluating the knowledge of liver cirrhosis among English-speaking adult patients.
Post-endoscopy colorectal cancer after colectomy in inflammatory bowel disease patients: a population-based register study
Authors: Stjärngrim Jessica , Widman Linnea , Schmidt Peter T , Ekbom Anders , Forsberg Anna 
Published: January 2023

Objectives
Long-standing inflammatory bowel disease (IBD) colitis is an indication for endoscopic surveillance. Postcolonoscopy colorectal cancer (PCCRC), cancer detected after a negative colonoscopy, is a quality indicator for colonoscopy. In analogy with PCCRC, we aimed to assess postendoscopy CRC (PECRC) in individuals with IBD who had undergone colectomy.

Methods
This register study included Swedish adults with an IBD diagnosis who had undergone colectomy and later were examined by either colonoscopy or sigmoidoscopy during 2001–2012. The final study population had a CRC diagnosis within 36 months of the index examination. Poisson regression was used to assess the relative risks (RR) of PECRC.

Results
A total of 33 individuals, 12 with an ileorectal anastomosis and 21 with a rectal remnant, had a CRC diagnosis within 36 months of the index endoscopy. Eleven cancers were detected as CRCs, and 22 (67%) were PECRCs. Compared with individuals aged >70 years, individuals aged <30 years had an RR of 3.1 (P = 0.054) and individuals aged 30–50 years had a RR of 2.6 (P = 0.030). A longer interval between colectomy and index endoscopy (>10 vs. <10 years) was associated with a lower risk of PCCRC (RR = 0.5; P = 0.007). There was no significant difference between the risk for Crohn’s disease vs. ulcerative colitis, or between ileorectal anastomosis and rectal remnant risks.

Conclusions
Continuous surveillance of IBD patients after colectomy is important. In the postcolectomy context, PECRC may be used as a quality indicator.