bluuebunny/crossref_metadata_2025_split huggingface dataset:
Illuminating Dark Chemical Matter using the Cell Painting Assay
Authors: Pahl Axel , Liu Jie , Patil Sohan , Rezaei Adariani Soheila , Schölermann Beate , Warmers Jens , Bonowski Jana , Koska Sandra , Sievers Sonja , Ziegler Slava , Waldmann Herbert 
Published: May 2023

AbstractThe identification of bioactive small molecules is at the heart of chemical biology and medicinal research. The screening for modulators of disease-relevant targets and phenotypes is the first step on the way to new drugs. Therefore, large compound libraries have been synthesized and employed by academia and, particularly, pharmaceutical companies to meet the need for chemical entities that are as diverse as possible. Extensive screening of these compound libraries revealed a portion of small molecules that is inactive in more than 100 different assays and was therefore termed ‘dark chemical matter’ (DCM). Deorphanization of DCM promises to yield very selective compounds as they, by definition, should have less off-target effects. We employed morphological profiling using the Cell painting assay (CPA) to detect bioactive DCM compounds. CPA is not biased to a given target or phenotype and can detect various unrelated mechanisms and modes of action. Within the DCM collection, we identified bioactive compounds and confirmed several modulators of microtubules, DNA synthesis and pyrimidine biosynthesis. Profiling approaches are therefore powerful tools to probe compound collections for bioactivity in an unbiased manner and particularly suitable for deorphanization of DCM.
Protein phosphatase 4 is required for Centrobin function in DNA damage repair
Authors: Réthi-Nagy Zsuzsánna , Ábrahám Edit , Sinka Rita , Juhász Szilvia , Lipinszki Zoltán 
Published: May 2023

AbstractGenome stability in human cells relies on the efficient repair of double-stranded DNA breaks, which is mainly achieved by homologous recombination (HR). Among the regulators of various cellular functions, Protein Phosphatase 4 (PP4) plays a pivotal role in coordinating the cellular response to DNA damage. Meanwhile, Centrobin (Ctb), initially recognized for its association with centrosomal function and microtubule dynamics, has sparked interest due to its potential contribution to DNA repair processes. In this study, we investigate the involvement of PP4 and its interaction with Ctb in HR-mediated DNA repair in human cells. Employing a range of experimental strategies, we investigate the physical interaction between PP4 and Ctb and shed light on the importance of two specific motifs in Ctb, the PP4-binding FRVP and the ATR kinase recognition SQ sequences, in the DNA repair process. Moreover, we examine cells lacking PP4 or Ctb and cells harboring FRVP and SQ mutations in Ctb, which result in similarly abnormal chromosome morphologies. This phenomenon likely results from the impaired resolution of Holliday junctions, which serve as crucial intermediates in HR. Taken together, our results provide new insights into the intricate mechanisms and interrelationships of PP4 and Ctb in the regulation of HR repair.
Particle-based phasor-FLIM-FRET resolves protein-protein interactions inside single viral particles
Authors: Coucke Quinten , Parveen Nagma , Fernández Guillermo Solís , Qian Chen , Hofkens Johan , Debyser Zeger , Hendrix Jelle 
Published: May 2023

AbstractFluorescence lifetime imaging microscopy (FLIM) is a popular modality to create additional contrast in fluorescence images. By carefully analyzing pixel-based nanosecond lifetime patterns, FLIM allows studying complex molecular populations. At the single molecule or single particle level, however, image series often suffer from low signal intensities per pixel, rendering it difficult to quantitatively disentangle different lifetime species, such as during FRET analysis in the presence of a significant donor-only fraction. To address this problem, we combined particle localization with phasor-based FLIM analysis. Using simulations, we first showed that an average of ∼300 photons, spread over the different pixels encompassing single fluorescing particles and without background, is enough to determine a correct phasor signature (standard deviation <5% for a 4 ns lifetime). For immobilized single- or double-labeled dsDNA molecules, we next validated that particle-based phasor-FLIM-FRET readily allows estimating fluorescence lifetimes and FRET from single molecules. Thirdly, we applied particle-based phasor-FLIM-FRET to investigate protein-protein interactions in sub diffraction HIV-1 viral particles. To do this, we first quantitatively compared the fluorescence brightness, lifetime and photostability of different popular fluorescent protein-based FRET probes when genetically fused to the HIV-1 integrase enzyme (IN) in viral particles, and conclude that eGFP, mTurquoise2 and mScarlet perform best. Finally, for viral particles co-expressing FRET-donor/acceptor labeled IN, we determined the absolute FRET efficiency of IN oligomers. Available in a convenient open-source graphical user interface, we believe that particle-based phasor-FLIM-FRET is a promising tool to provide detailed insights in samples suffering from low overall signal intensities.Why it mattersPhasor-FLIM is an extraordinarily popular tool for fluorescence lifetime imaging analysis. However, it remains susceptible for low signal intensities, operational challenges and therefore required informed users and a clear analysis understanding. In this work we developed a convenient all-graphical workflow for quantitative phasor-FLIM in heterogenous and low-signal samples and applied it to quantifying absolute FRET efficiencies from protein-protein interactions inside single viral particles. Moreover, containing a well-illustrated theoretical introduction to time-domain phasor-FLIM, our paper helps novice users to correctly implement phasor-FLIM in standard microscopy practice.
Human-specific lncRNAs contributed critically to human evolution by distinctly regulating gene expression
Authors: Lin Jie , Wen Yujian , Tang Ji , Zhang Xuecong , Zhang Huanlin , Zhu Hao 
Published: June 2023

ABSTRACTWhat genes and regulatory sequences make humans and apes that share substantial genes but show distinct phenotypes has puzzled researchers for decades. Genomic studies have examined species-specific genes and regulatory sequences (e.g., transcription factor binding sites, TFBS); birth, loss, and changes in these genes and sequences can greatly drive speciation and evolution. However, those involved in epigenetic regulation - species-specific lncRNA genes and regulatory sequences - remain poorly explored. We identified human-specific (HS) lncRNAs from GENCODE-annotated human lncRNAs, predicted their DNA binding domains (DBDs) and binding sites (DBSs) genome-wide, analyzed DBSs in modern humans (CEU, CHB, and YRI), archaic humans (Altai Neanderthals, Denisovans, and Vindija Neanderthals), and their counterparts in chimpanzees, and analyzed how DBSs influence gene expression in modern and archaic humans. Our results suggest that HS lncRNAs and their DBSs have substantially rewired gene expression human-specifically, the rewiring has evolved continuously from archaic to modern humans, and rewired gene expression has promoted brain development, made humans adapt to new environments and lifestyles, and caused differences in modern humans.
Local food heritage in a mountain tourism destination: evidence from the Alagna Walser Green Paradise project
Authors: Beltramo Riccardo , Bonadonna Alessandro , Duglio Stefano , Peira Giovanni , Vesce Enrica 
Published: June 2023

PurposeThis paper aims at assessing the role of authenticity of local food in contributing to tourism as an ecosystem service in a small mountain tourism destination, i.e. Alagna Valsesia (North-West of Italy).Design/methodology/approachA mix method approach has been adopted. The research has been carried out by using both CAWI (Computer Assisted Web Interview) and PAPI (Paper And Pen Interview) methodologies for surveys on the accommodation facilities managers and tourists. In addition, an analysis of electronic word-of-mouth platforms (TripAdvisor and Google) was implemented to understand more in-depth the local foodservice.FindingsFindings show that Alagna Valsesia embodies its authenticity with the tradition of the local culture, characterised by natural heritage and Walser uses. Some tools are identified to improve the attractiveness of the tourist destination.Research limitations/implicationsThe main limitation of this study can be found in the study' application to a rather limited and geographically restricted area.Practical implicationsThe results provided by this study can help local policy-makers in driving local policies and public funds in implementing ecotourism based practices.Originality/valueAlthough the role of local food has been much studied in relation to the tourism sector, to the knowledge of the authors, this contribution represents a first attempt to analyse local food's role in terms of local food's crossover contribution to supporting cultural tourism.
A comparative study on the perceptions of Dutch and British Muslim leadership regarding social-political challenges
Authors: Gökçekuyu Ertuğrul 
Published: May 2023

PurposeThis paper aims to analyze and compare attitudes and perceptions of Muslim leadership toward society and politics based on their personal experiences in England and the Netherlands.Design/methodology/approachThis study provides valuable insights into how Muslim leadership experiences socio-political realities from own perspectives. The comparative analysis of Muslim leadership in England and the Netherlands highlights the unique qualities of each community, while also demonstrating shared communal awareness regarding political participation and concerns regarding the rise of Islamophobia and populism.FindingsThe study reveals significant differences between Dutch and British Muslim leadership regarding attitudes toward politics. While the Dutch political system enables minorities to have political parties, British leaders prefer peaceful resistance, internal cooperation and voting power for influence. Muslim communities in both countries face similar challenges, such as access to politics, rising Islamophobia and populism. However, there are variations in leadership maturity and attitudes toward communal unification and political participation.Research limitations/implicationsEspecially Dutch Muslims were much more open toward cooperation once approached. The biggest challenge was to get into the Muslim communities in England. Even though British Muslims showed a much greater aptitude toward their civic duties, British Muslims were much more careful and wished that interviews were written down instead of being recorded. But once the author was part of the community, community leaders were more accepting and tolerant towards the author's presence. From that point on, to be recommended (snowballing) became easier.Practical implicationsThis research shows clearly how different ethnic Muslim communities differ across two different countries. Even though both countries have their own advantages and disadvantages, understanding how civic responsibilities and the progressive development of minority attitudes can be best approached is important to policymakers.Social implicationsOne major social implication is the way minorities perceive social and political equality and how these minority expectations can be met. Research shows that there is much fear and anxiety to growing sentiments of populism and right-wing appraisals. For a better integration and inclusion, a sound assessment of social and religious expectations is crucial.Originality/valueAs far as the author knows, there are no qualitative research studies that compare Muslim minority conditions in the Netherlands and England. This research makes this even more interesting for how to create policy that contributes to a European understanding of Muslim existence in Europe and a common European identity. This is crucial for a more stable and stronger European future.
Two-stage grey cloud clustering model under the panel data and its application
Authors: Luo Dang , Zhai Nana 
Published: June 2023

PurposeThe purpose of this paper is to establish a two-stage grey cloud clustering model under the panel data for the multi-attribute clustering problem with three-parameter interval grey number to evaluation of agricultural drought resistance grade of 18 cities in Henan Province.Design/methodology/approachThe clustering process is divided into two stages. In the first stage: Combining variance and time degree, the time weight optimization model is established. Applying the prospect theory, the index weight optimization model is established. Then, with the help of grey possibility function, the first stage of grey cloud clustering evaluation is carried out. In the second stage: the weight vector group of kernel clustering is constructed, and the grey class of the object is determined. A two-stage grey cloud clustering model under the panel data for the multi-attribute clustering problem is proposed.FindingsThis paper indicates that 18 cities in Henan Province are divided into four categories. The drought capacity in Henan province is high in the east and low in the west, high in the south and low in the north and the central region is relatively stable. The drought is greatly affected by natural factors. And the rationality and validity of this model is illustrated by comparing with other methods.Practical implicationsThis paper provides a practical method for drought resistance assessment, and provides theoretical support for farmers to grasp the drought information timely and improve the drought resistance ability.Originality/valueThe model in this paper solves the situation of ambiguity and randomness to some extent with the help of grey cloud possibility function. Moreover, the time weight of time degree and variance are used to reduce the volatility and the degree of subjective empowerment. Considering the risk attitude of the decision makers, the prospect theory is applied to make the index weight more objective. The rationality and validity of the model are illustrated by taking 18 cities in Henan Province as examples.
Service quality of online food delivery mobile application: an examination of the spillover effects of mobile app satisfaction
Authors: Lin Pearl M.C. , Au Wai Ching Wilson , Baum Thomas 
Published: June 2023

Purpose
Drawing on the mSERVQUAL model and spillover theory, this study aims to examine the mechanism through which food-ordering mobile app service quality influences users’ mobile app satisfaction, food satisfaction and repurchase intentions.

Design/methodology/approach
Online surveys were completed by 1,000 customers who used a food-ordering mobile app to order fast food on the day they completed the online survey. Structural equation modelling was then used to examine the proposed mechanism.

Findings
Results showed that the effects of food-ordering mobile app service quality on customer satisfaction (i.e. mobile app satisfaction and food satisfaction) and repurchase intention varied widely across service quality dimensions. Mobile app service quality had significant spillover effects on food satisfaction and repurchase intention.

Practical implications
Online food-delivery platforms should find the results insightful to better design their food-ordering mobile app. The findings can also assist restaurateurs and mobile payment companies with supporting the whole online food delivery process.

Originality/value
Rather than examining online food delivery service quality based on the service delivery process in the during-consumption stage or the service outcomes in the post-consumption stage, this study focused on the service quality in the pre-consumption stage to highlight the important role of online food delivery mobile apps. From a longitudinal perspective, this study drew on the associate network theory to explain the spillover effect of mobile app satisfaction in the pre-consumption stage on food satisfaction in the during-consumption stage and repurchase intention in the post-consumption stage.
Drivers of willingness to pay for reforestation of urban ecosystems in Ghana
Authors: Korle Kofi 
Published: June 2023

PurposePopulation growth and urbanization pose several threats to terrestrial ecosystems, especially in forest ecological zones worldwide. This study examines the drivers of average willingness to pay (WTP) to restore urban forests in a developing country.Design/methodology/approachIt utilizes survey data of households and employs a robust Heckman two-step estimator with bootstrapping to address the research objective.FindingsThe study underscores the role of income, gender, education and perception of the health benefits of forests as the underlying determinants of restoration bids by respondents. These drivers have a positive and statistically significant effect on forest restoration. Education and gender appear to be the most effective by magnitude, followed by the perception of health benefits, then income. Attention is therefore drawn to relevant economic, sociocultural and psychological factors towards the goal of forestry to improve well-being in urban centres.Originality/valueThis paper seeks to add methodological insights to the literature on reforestation and land use changes in the Accra metropolitan area and the local population’s WTP for reforestation in this area. In principle, this is a case study informing about the values people hold for forests in Ghana and Africa, where a knowledge gap exists with respect to their socio-economic valuation.Peer reviewThe peer review history for this article is available at: https://publons.com/publon/10.1108/IJSE-09-2022-0618
Can media richness and interaction act as stimulants to medical professionals’ learning persistence in MOOCs via fostering learning engagement?
Authors: Cheng Yung-Ming 
Published: May 2023

Purpose
The purpose of this study is to propose a research model based on the stimulus-organism-response (S-O-R) model to examine whether media richness (MR), human-system interaction (HSI) and human-human interaction (HHI) as technological feature antecedents to medical professionals’ learning engagement (LE) can affect their learning persistence (LP) in massive open online courses (MOOCs).

Design/methodology/approach
Sample data for this study were collected from medical professionals at six university-/medical university-affiliated hospitals in Taiwan. A total of 600 questionnaires were distributed, and 309 (51.5%) usable questionnaires were analyzed using structural equation modeling in this study.

Findings
This study certified that medical professionals’ perceived MR, HSI and HHI in MOOCs positively affected their emotional LE, cognitive LE and social LE elicited by MOOCs, which together explained their LP in MOOCs. The results support all proposed hypotheses and the research model accounts for 84.1% of the variance in medical professionals’ LP in MOOCs.

Originality/value
This study uses the S-O-R model as a theoretical base to construct medical professionals’ LP in MOOCs as a series of the psychological process, which is affected by MR and interaction (i.e. HSI and HHI). Noteworthily, three psychological constructs, emotional LE, cognitive LE and social LE, are adopted to represent medical professionals’ organisms of MOOCs adoption. To date, hedonic/utilitarian concepts are more commonly adopted as organisms in prior studies using the S-O-R model and psychological constructs have received lesser attention. Hence, this study enriches the S-O-R model into an invaluable context, and this study’s contribution on the application of capturing psychological constructs for completely explaining three types of technological features as external stimuli to medical professionals’ LP in MOOCs is well-documented.
Cui bono: a conversation with ChatGPT
Authors: Hirsch Peter Buell 
Published: May 2023

Purpose
To assess the ability of generative AI to assist in crisis management planning and response.

Design/methodology/approach
The viewpoint is built on a “conversation” with ChatGPT (CGPT) on the subject of crisis management. As such, portions of the text were generated by CGPT, a Large Language Model (LLM) and not by the author.

Findings
While CGPT has mastered the language of crisis management, its ability to assist in real-life situations is probably limited. Paradoxically, it believes it can help provide predictive analytics even though it claims not to be able to assess future events.

Originality/value
The author believes that the paradoxes inherent in CGPT’s claims to be able to assist in crisis management have not previously been examined.
Importance of meaningful engagement: how and why older adults volunteer in uncertain times
Authors: Dumont Renee , Sellon Alicia M. , Newsham Tina M.K. , Hollifield Mary C. , Thomas Alicia , Pate Melannie , Fugate-Whitlock Elizabeth 
Published: May 2023

Purpose
Many older adults engage in volunteer activities, drawing meaning and purpose through such efforts. Social distancing restrictions, put in place during Covid-19 surges to reduce the risk of transmission, disrupted older adult volunteers’ lives and volunteer experiences. Social distancing measures provide a unique opportunity to explore what happened when the choices around pausing or stopping volunteering were not entirely within the control of older adults. This paper aims to explore the experiences of older adult volunteers as they navigated uncertainties and made difficult decisions around balancing their safety and their desire to continue volunteering.

Design/methodology/approach
The authors conducted interviews with 26 community-dwelling older adults, age 50+, who had engaged in volunteer activities for at least 1 h a week prior to the start of the pandemic. The interviews were conducted on the phone or via Zoom. The authors used thematic analysis to help us analyze the data and identify patterns from participants’ experiences.

Findings
Despite the risk presented by Covid-19, most participants volunteered during the pandemic. They continued some or all of their previous activities with safety-related adjustments, with some seeking new or different opportunities. Participants’ discussions highlight the challenges of volunteering during the pandemic and the importance of engagement to their resiliency and subjective well-being.

Originality/value
This paper provides original contributions to understanding how and why older adults volunteered during the Covid-19 pandemic. The social distancing measures provide a novel opportunity to enrich our understanding of the meaningfulness and value of volunteerism to older adults’ lives and subjective well-being.
Rescripting creativity after automation: situating the simulacrum to interpret the queerness of computational creativity
Authors: Arantes Janine Aldous , Vicars Mark 
Published: June 2023

PurposeThe purpose of this paper is to examine how automation in the ever-changing technological landscape is increasing integrated into, and has become a significant presence in, our personal lives.Design/methodology/approachThrough post qualitative inquiry, the authors provide a contemplation of automation and its effect on creativity, as a contemporary expression of dis/locations, the simulacrum, performative work and a toxic digital presence in socio-cultural-technical spaces.FindingsThe authors discuss how we behave, contribute, explore, interact and communicate within and across automated digital platforms, has salience for understanding and questioning the ways that dominant discourses in the contemporary construction and enactment of subjectivity, creativity and agency are being modulated by the machine.Originality/valueThis paper offers a nuanced consideration of creativity, by considering the way creativity is being performed and situated within the effects of automation and its role in dis/locations, performative work and its potential as a the simulacrum in socio-cultural-technical spaces.