bluuebunny/crossref_metadata_2025_split huggingface dataset:
Investigation of the effect of MQL coolant temperature in AISI 316L steel turning using ANN-based optimization
Authors: benkhelifa oussama , Cherfia Abdelhakim , Nouioua Mourad , Hammoudi Abderzzak 
Published: January 2023

Abstract
In recent years, reducing the lubricant quantity used for the machining processes have gained much attention in order to limit the excessive use of conventional lubrication, for different considerations such as economic, ecological, and physical aspects. The minimum quantity lubrication (MQL) process is considered as economically, environmentally friendly and to be effective in overcoming this problem. Accordingly, this paper aims to analyze and evaluate the hard turning efficiency of AISI 316L Stainless steel with respect to surface roughness (Ra), and cutting temperature (Tc), according to combinations of cutting speed (Vc), feed rate (f), and cutting depth (ap) using coated carbide insert when turning of AISI 316L under dry, and MQL machining. It could be possible to investigate the efficiency of MQL technique for an environment-friendly ecological machining. The ANOVA analysis has been performed to determine the effect of cutting conditions on studied outputs. The results revealed that the cutting speed had the most effective influence on Ra followed by feed rate and lubrication mode, with contribution ratios of 58.39%, 19.92% and 11.91%, respectively. While the lubrication mode had the most influence on TC, with a contribution ratio of 88.98%.
Importance of Broken Geometric Symmetry of Single-Atom Pt Sites for Efficient Electrocatalysis
Authors: Cho Junsic , Lim Taejung , Kim Haesol , Meng Ling , Kim Jinjong , Lee Jong Hoon , Jung Gwan Yeong , Viñes Francesc , Illas Francesc , Exner Kai S. , Joo Sang Hoon , Choi Chang Hyuck 
Published: January 2023

Abstract
Platinum single-atom catalysts (SACs) hold promise as a new frontier in heterogeneous electrocatalysis. However, the exact chemical nature of active Pt sites is highly elusive, arousing many hypotheses to compensate for the significant discrepancies between experiments and theories. Here, we identify the stabilization of low-coordinated PtII species on carbon-based Pt SACs, which have rarely been found as reaction intermediates of homogeneous PtII catalysts, but have often been proposed as catalytic sites for Pt SACs from theory. Advanced online spectroscopic studies reveal multiple identities of PtII moieties on the SACs beyond ideally four-coordinated PtII–N4. Notably, decreasing Pt content to 0.15 wt.% enables the differentiation of low-coordinated PtII species from the four-coordinated ones, demonstrating their critical role in the chlorine evolution reaction (CER). This study suggests general guidelines for achieving high electrocatalytic performance of carbon-based SACs based on all other d8 metal ions, e.g., NiII and PdII as well as PtII.
Shatavari Supplementation in Postmenopausal Women Alters the Skeletal Muscle Proteome and Upregulates Proteins and Pathways Involved in Training Adaptation
Authors: O'Leary Mary , Jackman Sarah R , Bowtell Joanna L 
Published: January 2023

Abstract
Purpose
Shatavari is an understudied but widely available herbal supplement. It contains steroidal saponins and phytoestrogenic compounds. We previously showed that 6 weeks of shatavari supplementation improved handgrip strength and increased markers of myosin contractile function. Mechanistic insights into shatavari’s actions are limited. Therefore, we performed global proteomics on vastus lateralis (VL) samples that remained from our original study.
Methods
In a randomised double-blind trial, women (68.5 ± 6 years) ingested either placebo or shatavari (equivalent to 26,500 mg/d fresh weight) for 6 weeks. Tandem mass tag global proteomic analysis of VL samples was conducted (participants - N = 7 shatavari, N = 5 placebo). Data were normalised to total peptides and scaled using a reference sample across experiments. Data were filtered using a 5% FDR. Log2 transformed fold change (week 6 vs baseline) was calculated and Welch’s t-test performed. Over-representation (ORA) and pathway enrichment analyses (PADOG) were conducted in Reactome (v79).
Results
76 VL proteins were differentially expressed between placebo and shatavari. ORA demonstrated that proteins in pathways related to metabolism of proteins, amino acids and RNA were downregulated by shatavari. Proteins related to the pentose phosphate pathway were upregulated. PADOG showed that proteins in pathways related to integrin/MAPK signalling, cell growth, metabolism, apoptosis, elastic fibre formation, the neuronal system and chemical synapse transmission were significantly upregulated.
Conclusion
Our analyses indicate that shatavari may support muscle adaptation responses to exercise. These data provide useful signposts for future investigation of shatavari’s utility in conserving and enhancing musculoskeletal function. Trial registration NCT05025917 30/08/21, retrospectively registered.
Protectin conjugates in tissue regeneration 1 alleviates sepsis-induced acute lung injury by inhibiting ferroptosis
Authors: Lv Ya , Chen Deming , Tian Xinyi , Xiao Ji , Xu Congcong , Du Linan , Li Jiacong , Zhou Siyu , Chen Yuxiang , Zhuang Rong , Gong Yuqiang , Ying Binyu , Smith Fang Gao , Jin Shengwei , Gao Ye 
Published: January 2023

Abstract
Background
Acute lung injury (ALI) is a common and serious complication of sepsis with high mortality. Ferroptosis, categorized as programmed cell death, contributed to the development of lung injury. Protectin conjugates in tissue regeneration 1 (PCTR1) is an endogenous lipid mediator, exerting protective effects in multi-organ injury. However, the role of PCTR1 in the ferroptosis of sepsis-related ALI remains unknown.
Methods
Pulmonary epithelial cell line and the mouse model of ALI with lipopolysaccharides (LPS) stimulation were established in vitro and in vivo studies. Ferroptosis biomarkers including Fe2+, GSH, MDA and 4-HNE were detected by relevant assay kits. GPX4 and PTGS2 protein were determined by western blotting. Lipid peroxides were examined by fluorescence microscope and flow cytometry. Cell viability was detected by CCK-8 assay kit. Ultrastructure of mitochondria was observed with transmission electron microscopy. Morphology and inflammatory cytokine level predicted the severity of lung injury. Afterwards, related inhibitors were used to explore the potential mechanism by which PCTR1 regulated ferroptosis.
Results
PCTR1 treatment protected mice from LPS-induced lung injury, which was consisted with the effect of ferroptosis inhibitor ferrostatin-1. PCTR1 treatment decreased Fe2+, PTGS2 and lipid ROS contents, increased GSH and GPX4 levels and ameliorated mitochondrial ultrastructural injury. Administration of LPS or ferroptosis agonist RSL3 resulted in reduced cell viability, which was rescued by PCTR1. Mechanically, inhibition of PCTR1 receptor ALX, protein kinase A (PKA) and transcription factor cAMP-response element binding protein (CREB) partly decreased PCTR1 up-regulated GPX4 expression and CREB inhibitor blocked the effects of PCTR1 on ferroptosis inhibition and lung protection.
Conclusion
This study suggests that PCTR1 suppresses LPS-induced ferroptosis via ALX/PKA/CREB signalling pathway, which may offer a promising therapeutic prospect in the sepsis-related ALI.
Lociq: A Loci-Seeking Approach for Enhanced Plasmid Subtyping and Structural Characterization
Authors: Harrison Lucas , Zhao Shaohua , Li Cong , McDermott Patrick , Tyson Gregory , Strain Errol 
Published: January 2023

Abstract
Antimicrobial resistance (AMR) monitoring for public health is relying more on whole genome sequencing to characterize and compare resistant strains. This requires new approaches to describe and track AMR that take full advantage of the detailed data provided by genomic technologies. Tracking the plasmid-mediated spread of AMR genes is a primary concern for AMR monitoring. Sequence rearrangements can integrate new AMR genes into the plasmid backbone or promote hybridization of multiple plasmids. To better monitor plasmid evolution and dissemination, we developed a subtyping method to classify plasmids by variations in the sequence and arrangement of core plasmid genetic elements. This method generates multiple typing schema that both cluster related plasmids and characterize individual plasmids. This method provides an alpha-numeric nomenclature for plasmid subtyping to denominate plasmid diversity across multiple plasmid families. The Lociq method generates typing schema to track and characterize the origin, evolution and epidemiology of multidrug resistant plasmids.
QTL Mapping of Adult-Plant Resistance to Leaf and Stripe Rust in Wheat Cross L224-3/Zhengzhou5389
Authors: Li Zaifeng , Zhou Yue , Gao Pu , Miao Xiao-yan , Jiao Yue , Zhang Pei-pei , Cao Liu-qing 
Published: January 2023

Abstract

Wheat leaf rust and stripe rust are important diseases worldwide. Breading resistant cultivars is an effective means to control wheat leaf and stripe rust. Wheat line L224-3 currently has high resistance to wheat leaf and stripe rust in the field. In this study, 166 recombinant inbred lines (RILs) derived from the L224-3 × Zhengzhou 5389 cross were used to map quantitative trait locus (QTL) for leaf and stripe rust resistance. The population and two parents were phenotyped for leaf rust severity at Baoding in Hebei province and Zhoukou in Henan province in the 2015/2016 and 2016/2017 cropping seasons, and stripe rust severity at Baoding in Hebei Province and Mianyang in Sichuan Province in the 2015/2016 and 2016/2017 growth seasons. The population and parents were also genotyped with the 660K SNP array and simple sequence repeat (SSR) markers to screen for potential polymorphic markers associated with rust resistance. Four QTLs on chromosomes 1A, 2A, 4B and 7B, respectively, were detected using inclusive composite interval mapping (IciMapping). QLr.hbau-1A/QYr.hbau-1A, derived from susceptible parent Zhengzhou 5389, was pleiotropic for both leaf rust and stripe rust resistance and maybe a novel QTL. The second QTL on 2A in L224-3 for leaf and stripe rust resistance was the known gene Lr37/Yr17. QLr.hbau-4B/QYr.hbau-4B might be a new locus for leaf and stripe rust resistance. The last QTL for stripe rust resistance, tentatively designated as QYr.hbau-7B, was detected on 7B, and more tests are needed to confirm whether it is a new QTL. The QTL identified by their flanking markers in the present study could be used for candidate gene mining and marker-assisted selection (MAS) in wheat breeding programs for rust resistance.
A Novel Mutation in Rnf216 Gene in a Turkish Case With Gordon Holmes Syndrome
Authors: ÇELİK Nazlı DURMAZ , Erzurumluoğlu Ebru , Özben Serkan , Toprak Uğur , Yorulmaz Göknur , Artan Sevilhan , Özkan Serhat 
Published: January 2023

Abstract
Background: Gordon Holmes syndrome (GHS) is a rare autosomal recessive disorder characterized by hypogonadotropic hypogonadism, cognitive decline, and cerebellar ataxia. Mutations in the Ring Finger Protein 216 (RNF216) gene have been known to be associated with GHS therewithal RNF216 mutations have been detected in cases with Huntington-like disease, 4H syndrome (hypodontia, hypomyelination, ataxia and hypogonadotropic hypogonadism), and congenital hypogonadotropic hypogonadism.
Case Presentation: Here we report a novel homozygous frameshift mutation in RNF216 gene c.1860_1861dupCT (p.Cys621SerfsTer56) in a patient with hypogonadotropic hypogonadism, ataxia, and cognitive decline diagnosed with GHS also co-occurrence of parkinsonism and dystonia which was not reported before.
Conclusions: We report an extremely rare case of GHS. The core features of GHS are well defined, but genotype-phenotype correlations are still limited. To understand the pathophysiology of different phenotypes, the type and localization of novel mutations need to be defined, and the effect of these different variants on clinical features needs to be determined. Further studies should explain the factors of phenotypic variability present in GHS patients with RNF216 mutations.
Transperineal versus transrectal prostate biopsy in cancer detection rate for patients with gray-zone prostate-specific antigen: a multicenter, real-world study
Authors: Liu Junxiao , Niu Shaoxi , Wang Zeyuan , Sai Xiaoyong , Zhang Xuepei , Zhang Xu , Ma Xin 
Published: January 2023

Abstract

Background: The knowledge about the effect of different prostate biopsy approaches on the prostate cancer detection rate (CDR) for patients with gray-zone PSA is limited. We aimed to compare CDR in different biopsy approaches for patients with rising prostate-specific antigen (PSA) levels in the gray zone.
Methods: In this retrospective study, we identified 228 patients receiving transrectal prostate biopsy (TRB) compared with 216 receiving transperineal prostate biopsy (TPB) between 2016 and 2022. We also identified 110 patients receiving additional targeted biopsies following the systematic TPB. Clinical parameters were recorded, including age, PSA derivates, prostate volume (PV), and needle cores. Data was fitted into a propensity score matching (PSM), adjusting for potential confounders.
Results: TPB outperformed TRB in CDR (49.6% vs. 28.3%, P = 0.001). The clinically significant prostate cancer (CSPCa) detection rate showed no significant difference between TPB and TRB (78.6% vs. 68.8%, P = 0.306). In stratified analysis, TPB outperformed TRB in CDR when the age of patients was 65-75 years (59.0 % vs. 22.0%, P < 0.001), when PV was 25-50ml (63.2% vs. 28.3%, P < 0.001), and when needle cores were no more than 12 (58.5% vs. 31.4%, P = 0.005). The CDR (P = 0.712) and the detection rate of CSPCa (P = 0.993) showed no significant difference among systematic, targeted, and combined biopsies.
Conclusion: TPB outperformed TRB in CDR for patients with gray-zone PSA. However, targeted biopsy added no additional benefits following systematic TPB.
Exogenously applied silicon and zinc mitigates salt stress by improving leaf pigments and antioxidant activities in canola cultivars
Authors: Ahmad Zahoor , Khaliq Ayesha , Waraich Ejaz Ahmad , Artyszak Arkadiusz , Zaman Qamar uz , Abbasi Asim , Iqbal Muhammad Aamir , Alharby Hesham F. , Almaghamsi Afaf A. , Qamar Muhammad Javid 
Published: January 2023

Abstract
Globally, climate change and global warming induced salt stress is intensifying rapidly which has resulted in food supply constraints. The exogenous application of zinc (Zn) and silicon (Si) might serve as potent strategy to boost productivity of oil seed crops including canola, however research gaps exist regarding their dose optimization. A trial was conducted with an aim to determine the phenotypic divergence among canola cultivars under varying doses of Zn and Si applied solely and in conjunction with each other. The trail was comprised of two promising cultivars of canola (Sandal and Rachna) and seven foliar treatments including control, Si = 35 ppm, Si = 70 ppm, Zn = 35 ppm, Zn = 70 ppm, Si + Zn = 35 ppm and Si + Zn = 70 ppm applied under two level of salinity stress (SS). Different morphological and physiological traits of canola cultivars were taken as response variables. Completely Randomized Design (CRD) with factorial arrangement was used with three replications. The results revealed that SS adversely affected the leaf pigment and shoot length along with their fresh and dry weights, while antioxidant activities were increased especially under exogenous application of Si + Zn = 70 ppm. Additionally, root length (11.17 cm) and their fresh (2.63 g) and dry weights (0.78 g) were enhanced with the increasing SS level. Moreover, canola cv. Sandal outperformed in terms for shoot-root length and their fresh and dry weight as well as the leaf pigments contents. The co-application of Si + Zn = 70 ppm exhibited the highest shoot-root length (17.64 cm − 16.47 cm) and their fresh (2.60 g − 1.89 g) as well as dry weight (0.73 g − 0.29 g).The same treatment combination resulted in the maximum leaf pigments such as chlorophyll a (6.63 g/mg FW), chlorophyll b (4.37 g/mg FW) and total carotenoids content (2.38 g/mg FW).
Integrated study of HR-LC/MS and network pharmacology to identify breast cancer-related molecular targets of Fritillaria cirrhosa D. Don active constituents in combination with molecular dynamic simulation and experimental evaluation
Authors: Bhat Basharat Ahmad , Mir Wajahat Rashid , Alkhanani Mustfa , Almilaibary Abdullah , Mir Manzoor Ahmad 
Published: January 2023

AbstractFritillaria cirrhosaD. Don is a well-known medicinal plant in Kashmir Himalya. Traditionally, it has been used to treat several diseases, most notably in the treatment of various cancers particularly lung cancer. However, there is a significant gap between scientific research and its application in conventional medicine. The aim of the current work is to provide first-hand evidences bothin-vitroandin silicoshowing thatF. cirrhosaextracts exerts anti-cancer effects against breast cancer. Bulbs ofF. cirrhosawas extracted with various solvents of increasing polarity. Compounds were identified by High resolution-liquid chromatography-mass spectrometry (HR-LC/MS) technique. Phytocompounds were studied for protein targets involved in pathogenesis of breast cancer using Binding 1DB (similarity index > 0.7). Later, the protein-protein interactions (PPI) network was studied using STRING programme and compound-protein interactions using Cytoscape. In addition, molecular docking was used to investigate intermolecular interactions between the compounds and the proteins software using Autodock tool. Molecular dynamics simulations studies were also used to explore the stability of the representative CDK2 + Peiminine complex. In addition, standardin-vitrobiochemical assays were used to evaluate thein-vitroantiproliferative activity of active extracts ofF. cirrhosaagainst several breast cancer cell lines. Bioactive components and potential targets in the treatment of breast cancer were validated through network pharmacology approach. HR-LC/MS detected the presence of several secondary metabolites. Afterward, molecular docking was used to verify the effective activity of the active ingredients against the prospective targets. Additionally, Peiminine showed the highest binding energy score against CDK2 (-12.99 kcal/mol). CDK2 + Peiminine was further explored for molecular dynamics simulations. During the MD simulation study at 100 nanoseconds (ns), a stable complex formation of CDK2 + Peiminine was observed. According to molecular docking results predicted, several key targets of breast cancer bind stably with the corresponding phytocompounds ofF. cirrhosa. Lastly,F. cirrhosaextracts exhibited momentous anticancer activity throughin vitrostudies. Overall, the most important constituents were Imperialine-3-β-glucoside and Peiminine from theF. cirrhosabulbs has effective anti-cancer efficacy by deactivating Akt1 on the PI3K-Akt signaling pathway. Therefore, these findings emphasized the momentous anti-breast cancer activity ofF. cirrhosaextracts. This may open a new window and provide a theoretical foundation for further development and utilization ofF. cirrhosamedicinal plant in the treatment of breast cancer.
Experimental study on tilting deformation and a new method for landslide prediction with retaining-wall locked segment
Authors: Liu Handong , Liu Jingjing , Chen Jiaxing , Qiu Lei 
Published: January 2023

Abstract
The destruction of the locked-segment type landslide is often accompanied by the destruction of the locked segment with cumulative effects. These landslides quickly form high-speed, long-distance landslides and can be severe safety concerns. Physical models are used to study the evolution of retaining-wall locked segment landslides in the paper. HCA726S tilt sensors, micro earth pressure sensor, pore water pressure sensor and strain gauges were used to monitor the tilting deformation, slope stress, and failure characteristics of locked-segment type landslide. According to the landslide tilting deformation curve, strain, and earth pressure of the locked segment, the locked segment plays a crucial role in controlling the stability of a landslide. Here, the failure criterion of the locked segment is put forward. A landslide prediction model is proposed based on the angle-time curve of the tertiary creep stage. Using the improved angle tangent method, the landslide enters the creep stage of initial acceleration, medium acceleration, and high acceleration when the tilting angles are 0.34°, 1.89°, and 4.38°, respectively. The failure time of landslide with the locked segment predicted by the reciprocal velocity method is 5809 s, which is consistent with the actual time of the test (5800s).
Influence of Copper (I) Nicotinate Complex on the Notch1 Signaling Pathways in Triple Negative Breast Cancer Cell Lines
Authors: Abdel-Mohsen Mohamed A. , Badawy Asmaa , Abu-Youssef Morsy A. , Yehia Mona A. , Shamaa Lobna D. Abou , Abdullah Shymaa A. 
Published: January 2023

Abstract
TNBC is a subtype of breast cancer which is characterized by its aggressiveness, poor survival and short overall survival. Notch signaling pathway has received great attention as one of the most important potential targets for developing a novel therapeutic strategy.
Purpose, the present study is an attempt to assess the promising chemotherapeutic activities of copper (I) nicotinate (CNC) through its impact on the expression of downstream genes of Notch1 signaling pathway and the cell fate of TNBC. The co-treatment of TNBC cells with doxorubicin (Doxo) and CNC was also investigated.
Methods, to approach the objective of the present study, triple negative breast cancer HCC1806 and MDA MB 231 cell line were utilized. Cell viability assay, MTT assay, was used to determine IC50 values of CNC and Doxo. After treatment, cell cycle distribution and indirect antibody labeling of microtubule-associated protein light chain 3 (LC3) were determined by flow cytometry. Real time PCR was used to detect the changes in gene expressions that are involved Notch1 signaling pathway. Moreover, autophagosomes were monitored and imaged by TEM.
Results, treatment of TNBC cells with CNC modulated Notch1 signaling pathways in different manners with respect to the type of cells and the applied dose of CNC.
Conclusion, the observed pro-apoptotic of CNC in addition to its pro- or anti- autophagic activities may reflect the possible anti-cancer activities of CNC in both types of TNBC. However, cell type and CNC dose should be considered.
Effects of two different types of luteal support on pregnancy outcomes following antagonist fresh embryo transfer
Authors: De Minji , Chen Lixue , Zeng Lin , Wang Yang , Yang Rui , Li Rong , Chi Hongbin 
Published: January 2023

Abstract
Background: There are few reports on the use of progesterone vaginal gel in combination with dydrogesterone in the antagonist protocol for fresh embryo transfer. This study compared the effects of differing luteal support on the pregnancy outcomes following the antagonist protocol for fresh embryo transfer.
Methods: A retrospective analysis of clinical data from infertile patients who underwent fresh embryo transfer via the antagonist protocol (2785 cycles) from February to July 2019 and from February to July 2021 at the Peking University Third Hospital Reproductive Medicine Centre was performed. The groups were divided according to their luteal support into a progesterone vaginal gel group (single medication group; 1170 cycles) and progesterone vaginal gel plus dydrogesterone group (combination medication; 1615 cycles). After propensity score matching, the clinical pregnancy, ongoing pregnancy, early miscarriage, and ectopic pregnancy rates were compared between the two groups.
Results: A total of 1057 pairs of cycles were successfully propensity score matched. The clinical and ongoing pregnancy rates in the combination medication group were significantly higher than those in the single medication group (P < 0.05), whereas the differences in early miscarriage and ectopic pregnancy rates between the two groups were not significant (both P > 0.05).
Conclusions: Combined luteal support after the antagonist protocol is preferred for patients undergoing fresh cycle embryo transfer.