bluuebunny/crossref_metadata_2025_split huggingface dataset:
Prognostic value of neutrophil-to-lymphocyte ratio in metastatic colorectal cancer ⁎
Authors: Yu Qianqian , Qiu Hong , Zhang Mingsheng , Hu Guangyuan , Huang Liu , Yuan Xianglin 
Published: June 2023

Abstract

Objective
This study investigated the prognostic value of the neutrophil-to-lymphocyte ratio (NLR) in predicting clinical outcomes of patients with metastatic colorectal cancer (mCRC) undergoing irinotecanbased first-line chemotherapy.

Methods
This study was based on a Chinese prospective multicenter trial (NCT01282658). Baseline complete blood cell counts were performed. Survival was determined using the Kaplan-Meier method. Multivariate analyses based on the Cox regression model were performed to determine the effects of independent biomarkers.

Results
A total of 139 patients were evaluated. Values below the median NLR were associated with better progression-free survival (PFS) (9.9 vs 7.7 months, P = 0.043) and overall survival (OS) (21.8 vs 15.1 months, P = 0.013). These effects remained significant in multivariate analysis.

Conclusion
NLR is an independent prognostic marker of mCRC treated with first-line irinotecan-based therapy in a Chinese population.
Effect of parathyroid hormone on apoptosis of human medullary thyroid carcinoma cells*
Authors: Hou Xiaofeng , Liu Qinjiang , Tian Youxin 
Published: June 2023

Abstract

Objective
The aim of the study was to investigate the effect of parathyroid hormone (PTH) on the apoptosis of human medullary thyroid carcinoma (MTC) cells.

Methods

In vitro cultured medullary thyroid carcinoma cell lines were treated with parathyroid hormone and parathyroid hormone receptor-monoclonal antibody, and the apoptosis of cells was detected by flow cytometry.

Results
The cell morphology changed significantly after treatment based on the observation using the inverted phase-contrast microscope. Various concentrations of parathyroid hormone and parathyroid hormone receptor-monoclonal antibody effectively induced apoptosis in a time- and concentration-dependent manner. When the concentration of parathyroid hormone was 2.0 µmol/L and that of parathyroid hormone receptor-monoclonal antibody was 1.0 µmol/L, the apoptotic rate was 13.24% and 20.78%, respectively, representing a statistically significant difference from that of the control cells (P < 0.05).

Conclusion
PTH plays a role in inducing apoptosis of human MTC cells.
Papillary serous carcinoma of the uterine cervix: a clinicopathological analysis of 4 cases and a literature review
Authors: Ge Li , Yao Hongwen , Zhang Rong , Li Xiaoguang , Wu Lingying 
Published: June 2023

AbstractObjectiveTo investigate the clinicopathological characteristics and clinical treatment outcomes of patients with papillary serous carcinoma of the uterine cervix (PSCC).MethodsIn this study, 4 patients with histologically confirmed papillary serous carcinoma of the uterine cervix were retrospectively investigated. Pap smears, human papillomavirus (HPV) screening, tumor marker status, biopsy analysis, and relevant imaging examinations were conducted for the confirmation of primary diagnosis and recurrence. Patients underwent surgery, chemotherapy, or radiotherapy, and survival were the main endpoint.ResultsThe 4 patients were diagnosed with IB1, IB1, IIA, or IIIB disease. Two patients (2/4) presented with recurrence within 18 months after primary therapy. Compared with chemotherapy alone (progression-free survival (PFS): 11 months), radiotherapy combined with adjuvant chemotherapy showed favorable PFS rates (PFS: 20, 36, 13 months in 3 cases), although valid statistical analysis was not feasible because of the small sample size. The 5-year survival rate was 0%, and the 3-year survival rate was 75%. Our data, in agreement with the literature evidence, showed that the number of moderate-risk and high-risk factors in patients diagnosed with PSCC at an early stage was higher than that in patients diagnosed with common adenocarcinoma/squamous carcinoma of the uterine cervix.ConclusionPSCC has a poor clinical prognosis, and compared with chemotherapy alone, radiotherapy combined with adjuvant chemotherapy may lead to improved PFS.
Correlation between sodium-iodide symporter expression and circulating tumor cell positivity in differentiated thyroid carcinoma*
Authors: Wang Yunsheng , Liu Qinjiang , Tian Youxin 
Published: June 2023

AbstractObjectiveWe investigated the correlation between the expression of the sodium-iodide symporter (NIS) and the detection of circulating tumor cells (CTCs) in differentiated thyroid carcinoma (DTC).MethodsNIS expression in differentiated thyroid and the positive rate of CTCs in the peripheral blood were determined by immunohistochemistry S-P and flow cytometry from the records of 172 cases of differentiated thyroid carcinoma.ResultsSeventy-six cases (44.2%) expressed NIS in the differentiated thyroid and 63 cases (36.6%) were positive for CTCs in the peripheral blood. There was a significant difference between N0 and N1 in the expression of NIS (χ2= 6.015,P= 0.014) and the positive rate of CTCs (χ2= 14.035,P= 0.001). N0 and N1 also differed significantly in the expression of NIS (r= -0.383, -0.610,P= 0.002, < 0.001). The differences in the NIS expression, but not in the positive rate of CTCs, were significant among the different pathological subtypes (χ2= 7.897,P= 0.005; χ2= 1.455,P= 0.228, respectively). There was a significant negative correlation between the highly differentiated type and intermediate differentiation type both in the expression of NIS and positive rate of CTCs (r= -0.591, -0.443,P< 0.001,P= 0.002).ConclusionThere was a significant negative correlation between the expression of tissue NIS and positive rate of CTCs in the peripheral blood in DTC. The malignancy level and lymph node metastasis in differentiated thyroid carcinoma were negatively correlated with NIS expression and positively correlated with the positive rate of CTC.
Role of serum lactate dehydrogenase levels in evaluating efficacy of interventional therapy for hepatocellular carcinoma
Authors: Wang Zhihong , Ji Hong , Qiu Qiong , Wang Jianlan 
Published: June 2023

AbstractObjectiveThe aim of the study was to investigate the role of serum lactate dehydrogenase (LDH) levels in evaluating the efficacy of transcatheter arterial chemoembolization (TACE) for primary liver cancer.MethodsA total of 52 patients with liver cancer admitted in our hospital (Huangzhou District People's Hospital, Huanggang, China) from June 2015 to December 2017 were selected and divided into control group (LDH of ≤ 450 U/L,n= 26) and observation group (LDH of > 450 U/L,n= 26), based on the pretreatment level of LDH. Based on the changes in serum LDH levels before and after treatment, patients were classified into two groups: LDH increased group (22 cases) and LDH decreased group (30 cases). The relationship between LDH levels and efficacy of TACE treatment was analyzed in the four groups retrospectively.ResultsNo significant difference was seen in the clinical characteristics (gender, median age, performance status Eastern Cooperative Oncology Group, and staging system) between the control and observation groups. The efficacy rate in the control group was 57.7%, whereas that in the observation group was 42.3% (P> 0.05). The 1-year survival rate in the observation group was 53.8% and that in the control group was 84.6% (P< 0.05).ConclusionSerum LDH levels may be of clinical value in evaluating the efficacy of TACE in patients with hepatocellular carcinoma.
Correlation between miR-564, TGF-β1, and radiation-induced lung injury*
Authors: Ge Yunzhang , Xie Tao , Yang Bin , Li Qianxia , Ren Qingrong , Zhou Xiaoyi , Hu Desheng , Tu Zhongshu 
Published: June 2023

AbstractObjectiveOur study aimed to analyze the expression of miR-564 and TGF- β1 in cancer tissues and the serum of patients with radiation-induced lung injury, and to investigate the relationship between them and radiation-induced lung injury.MethodsIn situ hybridization and real-time fluorescence quantitative method were used to detect the expression of miR-564. Additionally, immunohistochemistry and enzyme-linked immunosorbent assay (ELISA) were performed to detect the expression of TGF- β1.ResultsThe overall incidence of acute radiation pneumonia was 55.9% (100/179). The incidence of ≥ grade 2 radioactive pneumonia was 24.0% (43/179) and that of grade 1 was 31.8% (57/179). The expression of miR-564 in grade ≥ 2 was slightly higher than that in patients without or with grade 1, but there was no statistical difference (P= 0.86). The serum level and ratio of miR-564 in patients with grade ≥ 2 were significantly higher than those without or with grade 1 (P= 0.005,P= 0.025, respectively). The expression of TGF- β1 in grade ≥ 2 was significantly higher than that of patients without or with grade 1 (P= 0.017). The serum levels of TGF- β1 in grade ≥ 2 were significantly higher than those in patients without or with grade 1 (P= 0.038). Although the ratio of TGF- β1 in radiation pneumonia of grade ≥ 2 was significantly higher than that of without or with grade 1, there was no significant difference (P= 0.24). Moreover, patients with higher expression of miR-564 and lower expression of TGF-β1 had better prognosis.ConclusionMiR-564 and TGF-β1 are predictors of radiation-induced lung injury. Monitoring its changing trend can improve the accuracy of predicting radiation-induced lung injury. The levels and ratio of serum miR-564 and TGF- β1 in patients with radiation-induced lung injury are related to the severity of radiation-induced lung injury.
Condylar invasion after postoperative chemotherapy for primary intraosseous squamous cell carcinoma of the mandible: a case report
Authors: Cheng Long , Li Qinyun , Du Guobo , Ma Daiyuan , Tan Bangxian 
Published: June 2023

AbstractPrimary intraosseous squamous cell carcinoma (PIOSCC) is a rare malignant tumor that occurs predominantly in the jaw. Pathological evidence shows that PIOSCC originates from the residual odontogenic epithelium and a preexisting odontogenic cyst or tumor. Here, we report the case of a 63-year-old man with central squamous cell carcinoma of the mandible. On the basis of imaging and histopathology reports, the patient was diagnosed with PIOSCC of the jaw. Subsequently, he was treated with postoperative adjuvant chemotherapy. During a postoperative follow-up visit in the ninth month, mandibular computed tomography (CT) scanning and bone imaging revealed local recurrence and condylar invasion. Therefore, radiation along with chemotherapy was administered. This case study adds to the literature on PIOSCC and widens the understanding of its diagnosis, treatment, and prognosis.
Analysis of cancer incidence and mortality data in Heilongjiang province cancer registries, China, 2015
Authors: Sun Huixin , Song Bingbing , Zhang Maoxiang 
Published: June 2023

AbstractObjectiveIn recent years, the rising incidence of cancer has increased patients' living and economic burdens. This study analyzed the incidence and death due to malignant tumors in tumor registries in Heilongjiang province (China) in 2015 to provide a scientific basis for the prevention and treatment of malignant tumors in this province.MethodsData on tumor incidence and patient deaths were collected from seven tumor registries in Heilongjiang province (China) in 2015. According to the stratification of urban and rural areas and patient sex, the crude, standard, and accumulative rates (0-74 years of age) were calculated. The 2000 China Population Census data and Segi's standard population were used to calculate the age-standardized rates.ResultsIn 2015, the incidence rate of malignant tumors in Heilongjiang cancer registries was 259.90/100 000. The age-standardized incidence rates in the Chinese and world standard populations were 158.89/100 000 and 155.06/100 000, respectively, with a cumulative incidence rate (0-74 years) of 17.68%. The incidence of malignant tumors in urban areas was 273.55/100 000, while that in rural areas was 220.32/100 000. The incidence of malignant tumors in men was 270.89/100 000, higher than that in women (249.04/100 000). Lung cancer had the highest incidence, followed by breast cancer, liver cancer, colorectal cancer, and thyroid cancer. The mortality rate of malignant tumors in Heilongjiang cancer registries was 164.69/100 000. The age-standardized mortality rates in Chinese and in world standard populations were 95.29/100 000 and 94.35/100 000, respectively, with a cumulative mortality rate (0-74 years) of 10.44%. The mortality rate of malignant tumors in urban areas was 169.51/100 000, while that in rural areas was 150.72/100 000. The mortality rate of malignant tumors in men was 201.64/100 000, higher than that in women (128.21/100 000). Lung cancer had the highest mortality, followed by liver cancer, stomach cancer, colorectal cancer, and breast cancer.ConclusionLung, liver, breast, and colorectal cancers were the most common cancers in Heilongjiang province, China, and should be considered the key cancer types for prevention and treatment. Moreover, the incidence of thyroid cancer is increasing, and thus early preventative measures should be implemented.
Radiation-induced brain injury after a conventional dose of intensity-modulated radiotherapy for nasopharyngeal carcinoma: a case report and literature review*
Authors: Zhang Qian , Tang Jie , Du Jiayu , Ma Xiaojie 
Published: June 2023

Abstract
A 61-year-old female nasopharyngeal carcinoma patient was admitted to the hospital with sudden cognitive dysfunction one month after Volumetric Intensity Modulated Arc Therapy (VMAT) conventional dose radiotherapy, and the initial diagnosis was radiation-induced brain injury (RBI). After comprehensive treatment with steroid hormones, the patient's condition rapidly improved. Typically, in nasopharyngeal carcinoma patients treated with VMAT, the incidence of RBI is extremely low when the temporal lobe dose is less than 65 Gy or 1% of the volume is less than 65 Gy. When this limit is exceeded, RBI may occur in varying degrees. However, in this case, even though the temporal lobe dose was under the prescribed limit, the patient still experienced RBI. The rare observations in this case can be used as a reference, and clinicians should seriously consider the possibility of RBI in similar cases.
Efficacy and adverse effects of olanzapine in the treatment of moderate to severe refractory neuropathic pain*
Authors: Dai Yuhong , Zou Man , Huang Tingting , Qiu Hong 
Published: June 2023

AbstractObjectiveThe aim of the study was to investigate the efficacy and adverse effects of olanzapine in the treatment of moderate to severe refractory neuropathic pain.MethodsForty patients with digestive system cancer were enrolled, who had moderate to severe refractory neuropathic pain; the patients were treated with olanzapine for 2 weeks at a daily dosage of 5 mg to 10 mg per night according to patients' response and tolerability, combined with conventional analgesic therapy. Pain intensity was evaluated by using a Numeral Rating Scale (NRS) at baseline, 3 days, and 2 weeks after therapy. The Pittsburg Sleep Quality Index (PSQI) was evaluated at baseline and 2 weeks after therapy. Data on adverse events were recorded. The dosage of conventional analgesics was adjusted over time based on the severity of pain.ResultsThe mean pain score decreased by 2.575 ± 1.318 (P< 0.000) at 3 days and by 3.400 ± 1.614 (P< 0.000) at 2 weeks; 30% of the patients experienced significant pain relief at 3 days and 50% at 2 weeks. The PSQI decreased by 4.725 ± 2.828 (P< 0.000) at 2 weeks. The adverse events induced by olanzapine included sleepiness, weight gain, dizziness, fatigue, dry mouth, and constipation; all the side effects were mild.ConclusionWhen combined with conventional analgesic therapy, olanzapine was effective in relieving pain and sleep disturbance, and was well-tolerated among patients with refractory neuropathic pain.
Effects of MIF on proliferation, migration, and STAT1 pathway of colon cancer cells
Authors: Liu Feng , Zhong Jianxin , Sun Jianbin , Wu Hailong 
Published: June 2023

Abstract

Objective
This study aimed to investigate how macrophage migration inhibitory factor (MIF) regulates the interaction of signal transducer and activator of transcription 1 (STAT1) with CD74, and affects colon cancer proliferation and invasion.

Methods
After transfecting MIF small interfering RNA into the SW480 cell line, the expression of STAT1 and CD74 mRNA was detected by qRT-PCR and western blotting. Transwell and MTT assays were performed to detect the colon cancer cell invasion and proliferation ability. Co-immunoprecipitation was used to detect the interaction between CD74 and STAT1 proteins in the treated and control groups.

Results
The cellular biological assays (MTT and Transwell) showed that the proliferation and invasion ability of colon cancer cells decreased after MIF knockdown; the results showed significant statistical difference (P < 0.05). The results of the co-immunoprecipitation assay suggested that MIF knockdown in colon cancer cells could inhibit the binding of CD74 and STAT1 proteins; statistical difference was observed between the two groups (P < 0.05).

Conclusion
MIF can increase the proliferation and invasion of colon cancer cells by promoting the combination of CD74 and STAT1.
The optional extent of lymph node dissection for pancreatic head cancer
Authors: Niu Guangcai , Ma Xiangdong 
Published: June 2023

Abstract

Objective
The extent of lymph node dissection for pancreatic head cancer (PHC) is uncertain and controversial; therefore, this study evaluated whether PHC patients can benefit from different extents of lymph node dissection.

Methods
A total of 106 PHC patients underwent standard regional lymphadenectomy (SRLN; n = 56, 52.8%) and extended regional lymphadenectomy (ERLN; n = 50, 47.2%) between September 2015 and September 2019. None of the study participants had distant metastases. The median survival time and complications were compared between the two groups.

Results
The median survival time in the SRLN and ERLN groups was 27.01 months and 21.17 months, respectively (P = 0.30). The postoperative major morbidity and mortality rates were 37.50% and 1.79% in the SRLN group, and 46.00% and 2.00% in the ERLN group, respectively. Moreover, the tumor differentiation, tumor diameter, lymph node involvement, perineural invasion, vascular invasion, and margin status all correlated with survival (P < 0.05).

Conclusion
For PHC patients, ERLN cannot provide a significant survival benefit over SRLN. Moreover, ERLN increased morbidity and mortality, although without statistical significance. This indicates that ERLN should not be considered in PHC patients.
Survival outcomes of patients with cervical esophageal cancer who received definitive radiotherapy: a retrospective study conducted in a single institution*
Authors: Wang Jing , Liu Fei , Wu Yingying , Zhou Lei , Hu Guangyuan , Yang Lin 
Published: June 2023

AbstractObjectiveCervical esophageal cancer (CEC) is a relatively rare condition, with limited treatment options. The current study aimed to assess the survival outcomes of patients with CEC who received definitive radiotherapy.MethodsIn total, 63 consecutive patients with CEC who received definitive radiotherapy between 2010 and 2018 were included in this study. The survival outcomes were analyzed based on statistics.ResultsThe median progression-free survival (PFS) and overall survival (OS) of the patients were 12 and 19 months, respectively. There were no significant differences in terms of survival outcomes between the groups who received radiation doses ≥ 60 and < 60 Gy. Interestingly, in the proximal CEC subgroup, the PFS (P= 0.039), OS (P= 0.031), and loco-regional failure-free survival (LRFFS) (P= 0.005) improved significantly in patients who received a radiation dose ≥ 60 Gy compared with those who received a radiation dose < 60 Gy. However, in the distal CEC subgroup, the PFS, OS, and LRFFS did not significantly improve between patients who received radiation doses ≥ 60 and < 60 Gy. Definitive radiotherapy was well tolerated, and no significant differences were observed in terms of treatment-related toxicities between the groups who received radiation doses ≥ 60 and < 60 Gy.ConclusionThe survival outcomes of patients with CEC should be improved. In proximal CEC, a radiation dose ≥ 60 Gy is significantly correlated with better PFS, OS, and LRFFS. However, further research must be performed to validate this finding.
Expression and role of PTV1 lncRNA in glioma cells progression*
Authors: Li Yue , Bai Yuling , Qi Yan , Cai Chang , Liao Ying , Liu Xiuzhu , He Pengchen 
Published: June 2023

Abstract

Objective
The aim of this study was to investigate the expression of PTV1 lncRNA in gliomas and the mechanism of its interaction with miR-203a.

Methods
U87 and U251 cells were cultured stably and transfected with sh-PTV1 or ov-PTV1, respectively. The proliferative activity of U87 and U251 cells was detected and the transplanted tumor model nude mice were divided into U87 and U251 groups. U87-sh and u251-ov cells were injected into the armpit, then miR-203a mic and miR-203a inhibitors were administered to detect the changes in the expression of tumor-related proteins.

Results
The relative expression of PTV1 in gliomas was significantly higher than that in normal brain tissues, while in GBM it was significantly higher than that in low-grade gliomas. Knockdown of PTV1 significantly inhibited the proliferation of U87 cells, resulting in fewer cell clones; overexpression of rPTV1 significantly promoted the proliferation of U251 cells, resulting in more cell colonies. The dual Luciferase Reporter assay showed that SP2 was a potential target of miR-203a. When U87 cells were treated with a miR-203a mimic, the expression of SP2 decreased; and when U251 cells were treated with a miR-203a inhibitor, the expression of SP2 increased significantly. SP2 was overexpressed in u87-sh cells and the proliferation, migration, and invasion of u87-sh cells were significantly enhanced. U251-ov cells showed the opposite trend. Compared with the control group mice, the tumor volume in u87-sh group mice was significantly smaller and the positive rate of SP2 in tumor tissue was significantly lower. After administration of the miR-203a inhibitor, the tumor volume increased gradually and the positive rate of SP2 increased significantly, while u251-ov mice showed the opposite trend.

Conclusion
lncRNA PTV1 can be used as a molecule to interfere with miR-203a expression in order to downregulate SP2 and to promote the proliferation and invasion of glioma cells. lncRNA PTV1 may be a new biomarker and therapeutic target for glioma.