bluuebunny/crossref_metadata_2025_split huggingface dataset:
Autoimmune Congenital Complete Heart Block: How Late Can It Occur?
Authors: Makadia Luv , Izmirly Peter , Buyon Jill P. , Phoon Colin K. L. 
Published: May 2023

Abstract
Objective Maternal anti-Ro (SSA) and/or anti-La (SSB) antibodies are a risk factor for congenital complete heart block (CHB). Because detailed analysis of the incidence of CHB after 24 weeks of gestational age (GA) is lacking, we aimed to ascertain the risk of “later-onset” CHB among offspring of SSA/SSB-positive mothers in the published literature.
Study Design Using search terms “neonatal lupus heart block” and “autoimmune congenital heart block” on PubMed and Ovid, we gathered prospective studies of SSA/SSB-positive mothers with fetal echo surveillance starting from before CHB diagnosis and retrospective cases of fetal CHB diagnosis after 24 weeks of GA (if there was prior normal heart rate) or after birth.
Results Ten prospective studies included 1,248 SSA/SSB-positive pregnancies with 24 cases of CHB diagnosed during pregnancy (1.9%). Among these, three (12.5%) were after 24 weeks—at weeks 25, 26, and 28. Our retrospective studies revealed 50 patients with CHB diagnosis in late fetal life and neonatal period and 34 in the nonneonatal childhood period. An additional four cases were diagnosed after age 18 years.
Conclusion Later-onset autoimmune CHB in offspring of SSA/SSB-positive mothers does occur. Our analysis suggests that prenatal surveillance should continue beyond 24 weeks of GA but is limited by inconsistent published surveillance data.
Risk mitigation services in cyber insurance: optimal contract design and price structure
Authors: Zeller Gabriela , Scherer Matthias 
Published: May 2023

AbstractAs the cyber insurance market is expanding and cyber insurance policies continue to mature, the potential of including pre-incident and post-incident services into cyber policies is being recognised by insurers and insurance buyers. This work addresses the question of how such services should be priced from the insurer’s viewpoint, i.e. under which conditions it is rational for a profit-maximising, risk-neutral or risk-averse insurer to share the costs of providing risk mitigation services. The interaction between insurance buyer and seller is modelled as a Stackelberg game, where both parties use distortion risk measures to model their individual risk aversion. After linking the notions of pre-incident and post-incident services to the concepts of self-protection and self-insurance, we show that when pricing a single contract, the insurer would always shift the full cost of self-protection services to the insured; however, this does not generally hold for the pricing of self-insurance services or when taking a portfolio viewpoint. We illustrate the latter statement using toy examples of risks with dependence mechanisms representative in the cyber context.
Review symposium: Beyond presidentialism and parliamentarism
Authors: Tsebelis George , Thies Michael , Cheibub José Antonio , Dixon Rosalind , Bogéa Daniel , Ganghof Steffen 
Published: May 2023

AbstractSteffen Ganghof’s Beyond Presidentialism and Parliamentarism: Democratic Design and the Separation of Powers (Oxford University Press, 2021) posits that “in a democracy, a constitutional separation of powers between the executive and the assembly may be desirable, but the constitutional concentration of executive power in a single human being is not” (Ganghof, 2021). To consider, examine and theorise about this, Ganghof urges engagement with semi-parliamentarism. As explained by Ganghof, legislative power is shared between two democratically legitimate sections of parliament in a semi-parliamentary system, but only one of those sections selects the government and can remove it in a no-confidence vote. Consequently, power is dispersed and not concentrated in the hands of any one person, which, Ganghof argues, can lead to an enhanced form of parliamentary democracy. In this book review symposium, George Tsebelis, Michael Thies, José Antonio Cheibub, Rosalind Dixon and Daniel Bogéa review Steffen Ganghof’s book and engage with the author about aspects of research design, case selection and theoretical argument. This symposium arose from an engaging and constructive discussion of the book at a seminar hosted by Texas A&M University in 2022. We thank Prof José Cheibub (Texas A&M) for organising that seminar and Dr Anna Fruhstorfer (University of Potsdam) for initiating this book review symposium.
How can we foster caring meals? Findings from an ethnographic study in day care centers for older adults in Sápmi, Norway
Authors: Holster Terhi , Munkejord Mai Camilla 
Published: May 2023

AbstractThis study aims to advance our understanding of meals in Day Care Centers (DCCs) for older adults. By using a phenomenological approach, we aim to shed light on physical, sociocultural, and existential aspects of participants’ bodily experiences of meal situations. Based on a thematic analysis of qualitative data from seven weeks of ethnographic fieldwork in two DCCs for older adults in Sápmi, Norway (2021–2022), we explore how we can foster nourishing, meaningful, and diversity-sensitive meals, adjusted to the bodies cared for. In our conclusion, we argue that food should be considered an important part of care in DCCs—not only as a means to feed older adults but as a way to nourish the whole person cared for.
Development of NOTCH, an all-electron, beyond-NDDO semiempirical method: Application to diatomic molecules
Authors: Wang Zikuan , Neese Frank 
Published: May 2023

In this work, we develop a new semiempirical method, dubbed NOTCH (Natural Orbital Tied Constructed Hamiltonian). Compared to existing semiempirical methods, NOTCH is less empirical in its functional form as well as parameterization. Specifically, in NOTCH, (1) the core electrons are treated explicitly; (2) the nuclear–nuclear repulsion term is calculated analytically, without any empirical parameterization; (3) the contraction coefficients of the atomic orbital (AO) basis depend on the coordinates of the neighboring atoms, which allows the size of AOs to depend on the molecular environment, despite the fact that a minimal basis set is used; (4) the one-center integrals of free atoms are derived from scalar relativistic multireference equation-of-motion coupled cluster calculations instead of empirical fitting, drastically reducing the number of necessary empirical parameters; (5) the (AA|AB) and (AB|AB)-type two-center integrals are explicitly included, going beyond the neglect of differential diatomic overlap approximation; and (6) the integrals depend on the atomic charges, effectively mimicking the “breathing” of AOs when the atomic charge varies. For this preliminary report, the model has been parameterized for the elements H–Ne, giving only 8 empirical global parameters. Preliminary results on the ionization potentials, electron affinities, and excitation energies of atoms and diatomic molecules, as well as the equilibrium geometries, vibrational frequencies dipole moments, and bond dissociation energies of diatomic molecules, show that the accuracy of NOTCH rivals or exceeds those of popular semiempirical methods (including PM3, PM7, OM2, OM3, GFN-xTB, and GFN2-xTB) as well as the cost-effective ab initio method Hartree–Fock-3c.
Neotype kuramite optoelectronic memristor for bio-synaptic plasticity simulations
Authors: Dong Xiaofei , Wei Wenbin , Sun Hao , Li Siyuan , Chen Jianbiao , Chen Jiangtao , Zhang Xuqiang , Zhao Yun , Li Yan 
Published: May 2023

Memristive devices with both electrically and optically induced synaptic dynamic behaviors will be crucial to the accomplishment of brain-inspired neuromorphic computing systems, in which the resistive materials and device architectures are two of the most important cornerstones, but still under challenge. Herein, kuramite Cu3SnS4 is newly introduced into poly-methacrylate as the switching medium to construct memristive devices, and the expected high-performance bio-mimicry of diverse optoelectronic synaptic plasticity is demonstrated. In addition to the excellent basic performances, such as stable bipolar resistive switching with On/Off ratio of ∼486, Set/Reset voltage of ∼−0.88/+0.96 V, and good retention feature of up to 104 s, the new designs of memristors possess not only the multi-level controllable resistive-switching memory property but also the capability of mimicking optoelectronic synaptic plasticity, including electrically and visible/near-infrared light-induced excitatory postsynaptic currents, short-/long-term memory, spike-timing-dependent plasticity, long-term plasticity/depression, short-term plasticity, paired-pulse facilitation, and “learning-forgetting-learning” behavior as well. Predictably, as a new class of switching medium material, such proposed kuramite-based artificial optoelectronic synaptic device has great potential to be applied to construct neuromorphic architectures in simulating human brain functions.
Comparative metabolomics analysis shows key metabolites as potential biomarkers for selection of beef fat colour
Authors: Tian Rugang , Kharrati-Koopaee Hamed , Asadollahpour Nanaie Hojjat , Wang Xiao , Zhao Meng , Li Hui , Li Yuan , Zhang Hao , Esmailizadeh Ali , Bottema Cynthia D. K. 
Published: May 2023

Context Fat colour is one of the most important economic traits in the marketing of beef. There are many factors that affect fat colour, such as breed, age, diet and gender. Fat colour is observed in different ranges of colours, including white, yellow and brown. The main issue with improving fat colour is that consumer preferences of fat colour vary across the globe. Therefore, investigating the metabolic mechanisms of fat colour may provide new biomarkers for phenotyping, so as to develop effective selection strategies to achieve the locally desired fat colour. Aims This study aimed to perform a comparative metabolic analysis between white and yellow fat from crossbred cattle so as to identify potential biomarkers for the selection of fat colour and to better understand the metabolism of white and yellow fat depots. Methods Carcass samples of subcutaneous fat were collected from crossbred cattle (Simmental × Mongolian cattle) and scored for fat colour. Liquid chromatography–mass spectrophotometry analysis of extracted metabolites from the subcutaneous fat of six animals with white fat and six animals with yellow fat was performed. Key results The comparison between metabolites of white and yellow fat colour samples indicated that there were five categories of 235 significant metabolites, which included hydrocarbons, lipids and lipid-like molecules, organic acids and their derivatives, organic oxygen compounds and organoheterocyclic compounds. The principal-component analysis illustrated that yellow and white fat samples can be classified in groups; however, the metabolites of white fat samples showed greater variation than those in the yellow fat. In the white fat, there were 163 metabolites that had a higher relative abundance than in yellow fat and 72 that had a lower relative abundance than in yellow fat. 3-Hydroxyoctanoic acid, anethofuran, 9,10-DiHODE, furanoeremophilane, pregeijerene, N-glycolylneuraminic acid, and glycocholic acid were identified as the metabolites that differed the most in abundance between the white and yellow fat samples. Conclusions This study has provided insights into the metabolic differences between white and yellow fat depots and identified key metabolites associated with beef fat colour. Implications This study has provided potential biomarkers that may be useful for selection of beef fat colour in live animals.
Chemiexcitation and melanin in photoreceptor disc turnover and prevention of macular degeneration
Authors: Lyu Yanan , Tschulakow Alexander V. , Wang Kun , Brash Douglas E. , Schraermeyer Ulrich 
Published: May 2023

Age-related macular degeneration, Stargardt disease, and their

Abca4
-/-

mouse model are characterized by accelerated accumulation of the pigment lipofuscin, derived from photoreceptor disc turnover in the retinal pigment epithelium (RPE); lipofuscin accumulation and retinal degeneration both occur earlier in albino mice. Intravitreal injection of superoxide (O
2
•–
) generators reverses lipofuscin accumulation and rescues retinal pathology, but neither the target nor mechanism is known. Here we show that RPE contains thin multi-lamellar membranes (TLMs) resembling photoreceptor discs, which associate with melanolipofuscin granules in pigmented mice but in albinos are 10-fold more abundant and reside in vacuoles. Genetically over-expressing tyrosinase in albinos generates melanosomes and decreases TLM-related lipofuscin. Intravitreal injection of generators of O
2
•–
or nitric oxide (
•
NO) decreases TLM-related lipofuscin in melanolipofuscin granules of pigmented mice by ~50% in 2 d, but not in albinos. Prompted by evidence that O
2
•–
plus
•
NO creates a dioxetane on melanin that excites its electrons to a high-energy state (termed “chemiexcitation”), we show that exciting electrons directly using a synthetic dioxetane reverses TLM-related lipofuscin even in albinos; quenching the excited-electron energy blocks this reversal. Melanin chemiexcitation assists in safe photoreceptor disc turnover.
ATG5 selectively engages virus-tethered BST2/tetherin in an LC3C-associated pathway
Authors: Judith Delphine , Versapuech Margaux , Bejjani Fabienne , Palaric Marjory , Verlhac Pauline , Kuster Aurelia , Lepont Leslie , Gallois-Montbrun Sarah , Janvier Katy , Berlioz-Torrent Clarisse 
Published: May 2023

Bone marrow stromal antigen 2 (BST2)/tetherin is a restriction factor that reduces HIV-1 dissemination by tethering virus at the cell surface. BST2 also acts as a sensor of HIV-1 budding, establishing a cellular antiviral state. The HIV-1 Vpu protein antagonizes BST2 antiviral functions via multiple mechanisms, including the subversion of an LC3C-associated pathway, a key cell intrinsic antimicrobial mechanism. Here, we describe the first step of this viral-induced LC3C-associated process. This process is initiated at the plasma membrane through the recognition and internalization of virus-tethered BST2 by ATG5, an autophagy protein. ATG5 and BST2 assemble as a complex, independently of the viral protein Vpu and ahead of the recruitment of the ATG protein LC3C. The conjugation of ATG5 with ATG12 is dispensable for this interaction. ATG5 recognizes cysteine-linked homodimerized BST2 and specifically engages phosphorylated BST2 tethering viruses at the plasma membrane, in an LC3C-associated pathway. We also found that this LC3C-associated pathway is used by Vpu to attenuate the inflammatory responses mediated by virion retention. Overall, we highlight that by targeting BST2 tethering viruses, ATG5 acts as a signaling scaffold to trigger an LC3C-associated pathway induced by HIV-1 infection.
MWF of the corpus callosum is a robust measure of remyelination: Results from the ReBUILD trial
Authors: Caverzasi Eduardo , Papinutto Nico , Cordano Christian , Kirkish Gina , Gundel Tristan J. , Zhu Alyssa , Akula Amit Vijay , Boscardin W. John , Neeb Heiko , Henry Roland G. , Chan Jonah R. , Green Ari J. 
Published: May 2023

Myelin repair is an unrealized therapeutic goal in the treatment of multiple sclerosis (MS). Uncertainty remains about the optimal techniques for assessing therapeutic efficacy and imaging biomarkers are required to measure and corroborate myelin restoration. We analyzed myelin water fraction imaging from ReBUILD, a double-blind, randomized placebo-controlled (delayed treatment) remyelination trial, that showed a significant reduction in VEP latency in patients with MS. We focused on brain regions rich in myelin. Fifty MS subjects in two arms underwent 3T MRI at baseline and months 3 and 5. Half of the cohort was randomly assigned to receive treatment from baseline through 3 mo, whereas the other half received treatment from 3 mo to 5 mo post-baseline. We computed myelin water fraction changes occurring in normal-appearing white matter of corpus callosum, optic radiations, and corticospinal tracts. An increase in myelin water fraction was documented in the normal-appearing white matter of the corpus callosum, in correspondence with the administration of the remyelinating treatment clemastine. This study provides direct, biologically validated imaging-based evidence of medically induced myelin repair. Moreover, our work strongly suggests that significant myelin repair occurs outside of lesions. We therefore propose myelin water fraction within the normal-appearing white matter of the corpus callosum as a biomarker for clinical trials looking at remyelination.
A new capacity of gut microbiota: Fermentation of engineered inorganic carbon nanomaterials into endogenous organic metabolites
Authors: Cui Xuejing , Wang Xiaoyu , Chang Xueling , Bao Lin , Wu Junguang , Tan Zhiqiang , Chen Jinmei , Li Jiayang , Gao Xingfa , Ke Pu Chun , Chen Chunying 
Published: May 2023

Carbon-based nanomaterials (CNMs) have recently been found in humans raising a great concern over their adverse roles in the hosts. However, our knowledge of the in vivo behavior and fate of CNMs, especially their biological processes elicited by the gut microbiota, remains poor. Here, we uncovered the integration of CNMs (single-walled carbon nanotubes and graphene oxide) into the endogenous carbon flow through degradation and fermentation, mediated by the gut microbiota of mice using isotope tracing and gene sequencing. As a newly available carbon source for the gut microbiota, microbial fermentation leads to the incorporation of inorganic carbon from the CNMs into organic butyrate through the pyruvate pathway. Furthermore, the butyrate-producing bacteria are identified to show a preference for the CNMs as their favorable source, and excessive butyrate derived from microbial CNMs fermentation further impacts on the function (proliferation and differentiation) of intestinal stem cells in mouse and intestinal organoid models. Collectively, our results unlock the unknown fermentation processes of CNMs in the gut of hosts and underscore an urgent need for assessing the transformation of CNMs and their health risk via the gut-centric physiological and anatomical pathways.
Imaging of pH distribution inside individual microdroplet by stimulated Raman microscopy
Authors: Gong Kedong , Ao Jianpeng , Li Kejian , Liu Le , Liu Yangyang , Xu Guanjun , Wang Tao , Cheng Hanyun , Wang Zimeng , Zhang Xiuhui , Wei Haoran , George Christian , Mellouki Abdelwahid , Herrmann Hartmut , Wang Lin , Chen Jianmin , Ji Minbiao , Zhang Liwu , Francisco Joseph S. 
Published: May 2023

Aerosol microdroplets as microreactors for many important atmospheric reactions are ubiquitous in the atmosphere. pH largely regulates the chemical processes within them; however, how pH and chemical species spatially distribute within an atmospheric microdroplet is still under intense debate. The challenge is to measure pH distribution within a tiny volume without affecting the chemical species distribution. We demonstrate a method based on stimulated Raman scattering microscopy to visualize the three-dimensional pH distribution inside single microdroplets of varying sizes. We find that the surface of all microdroplets is more acidic, and a monotonic trend of pH decreasing is observed in the 2.9-μm aerosol microdroplet from center to edge, which is well supported by molecular dynamics simulation. However, bigger cloud microdroplet differs from small aerosol for pH distribution. This size-dependent pH distribution in microdroplets can be related to the surface-to-volume ratio. This work presents noncontact measurement and chemical imaging of pH distribution in microdroplets, filling the gap in our understanding of spatial pH in atmospheric aerosol.
Upregulation of the ERRγ–VDAC1 axis underlies the molecular pathogenesis of pancreatitis
Authors: Chanda Dipanjan , Thoudam Themis , Sinam Ibotombi Singh , Lim Chae Won , Kim Myeongjin , Wang Jiale , Lee Kyeong-Min , Ma Jing , Saxena Romil , Choi Jinhyuk , Oh Chang Joo , Lee Hoyul , Jeon Yong Hyun , Cho Sung Jin , Jung Hoe-Yune , Park Keun-Gyu , Choi Hueng-Sik , Suh Jae Myoung , Auwerx Johan , Ji Baoan , Liangpunsakul Suthat , Jeon Jae-Han , Lee In-Kyu 
Published: May 2023

Emerging evidence suggest that transcription factors play multiple roles in the development of pancreatitis, a necroinflammatory condition lacking specific therapy. Estrogen-related receptor γ (ERRγ), a pleiotropic transcription factor, has been reported to play a vital role in pancreatic acinar cell (PAC) homeostasis. However, the role of ERRγ in PAC dysfunction remains hitherto unknown. Here, we demonstrated in both mice models and human cohorts that pancreatitis is associated with an increase in ERRγ gene expression via activation of STAT3. Acinar-specific ERRγ haploinsufficiency or pharmacological inhibition of ERRγ significantly impaired the progression of pancreatitis both in vitro and in vivo. Using systematic transcriptomic analysis, we identified that voltage-dependent anion channel 1 (VDAC1) acts as a molecular mediator of ERRγ. Mechanistically, we showed that induction of ERRγ in cultured acinar cells and mouse pancreata enhanced VDAC1 expression by directly binding to specific site of the
Vdac1
gene promoter and resulted in VDAC1 oligomerization. Notably, VDAC1, whose expression and oligomerization were dependent on ERRγ, modulates mitochondrial Ca
2+
and ROS levels. Inhibition of the ERRγ–VDAC1 axis could alleviate mitochondrial Ca
2+
accumulation, ROS formation and inhibit progression of pancreatitis. Using two different mouse models of pancreatitis, we showed that pharmacological blockade of ERRγ–VDAC1 pathway has therapeutic benefits in mitigating progression of pancreatitis. Likewise, using PRSS1
R122H
-Tg mice to mimic human hereditary pancreatitis, we demonstrated that ERRγ inhibitor also alleviated pancreatitis. Our findings highlight the importance of ERRγ in pancreatitis progression and suggests its therapeutic intervention for prevention and treatment of pancreatitis.
Anterior cingulate cortex orexin signaling mediates early-life stress-induced social impairment in females
Authors: Luo Fei , Deng Jun-yang , Sun Xuan , Zhen Jian , Luo Xiao-dan 
Published: May 2023

Early-life stress has long-term impacts on the structure and function of the anterior cingulate cortex (ACC), and raises the risk of adult neuropsychiatric disorders including social dysfunction. The underlying neural mechanisms, however, are still uncertain. Here, we show that, in female mice, maternal separation (MS) during the first three postnatal weeks results in social impairment accompanied with hypoactivity in pyramidal neurons (PNs) of the ACC. Activation of ACC PNs ameliorates MS-induced social impairment. Neuropeptide Hcrt, which encodes hypocretin (orexin), is the top down-regulated gene in the ACC of MS females. Activating ACC orexin terminals enhances the activity of ACC PNs and rescues the diminished sociability observed in MS females via an orexin receptor 2 (OxR2)-dependent mechanism. Our results suggest orexin signaling in the ACC is critical in mediating early-life stress-induced social impairment in females.