bluuebunny/crossref_metadata_2025_split huggingface dataset:
Traction force with extracellular matrix mediated by cytoskeleton had an effect on metastasis through SLC8A1 induced Wnt-β-catenin pathway in endometrial cancer
Authors: Li Xingchen , Xu Xin , Zhou Jingyi , Wang Jianliu 
Published: March 2023

Abstract
Endometrial cancer (EC) is a common malignant tumor of female reproductive system. It seriously affects women's health. The mechanical characteristics of EC have not been paid noticed. However, the mechanical characteristics of different types of EC are diverse. These characteristics will greatly influence the development and prognosis of cancer cells. In this study, we explored traction force in primary EC cells and EC cell lines for the first time. It was found that the traction force of primary EC cells or cell lines with metastatic capability was lower. Various clinicopathological features of EC patients are significantly associated with traction force. In addition, we also confirmed that SLC8A1 is the main molecule which had an effect on traction force. The stiffness of extracellular matrix (ECM) had an influence on its downstream cytoskeleton expression through SLC8A1, thus changing the traction force and metastatic capacity of EC cells. The mechanism behind the SLC8A1 may be related to Wnt-β- catenin pathway and ion-channel activity. SLC8A1 can significantly regulate the downstream cytoskeleton F-actin and promote EC cell metastasis. Therefore, in this study, we found that SLC8A1 is an important molecule that affects the mechanical characteristics of endometrial cancer. The molecular mechanism is through changing the cytoskeleton by regulating Wnt- β- catenin pathway.
Exposure of the static magnetic fields on the microbial growth rate and sludge properties in the complete-mix activated sludge process (a Lab-scale study)
Authors: Asgari Ghorban , Seid-Mohammadi Abdolmotaleb , Shokoohi Reza , Samarghandi Mohammad Reza , Diger Glen T , Malekolkalami Behrooz , Khoshniyat Ramin 
Published: March 2023

Abstract
In this study, the effect of static magnetic fields (SMFs) on improving the performance of the activated sludge to enhance the microbial growth rate and improve sludge settling characteristics in the real operation conditions has been investigated. The effect of SMFs (15 mT), hydraulic retention time, the sludge age, the aeration time on mixed liquor suspended solids (MLSS), mixed liquor volatile suspended solids (MLVSS), α-factor, and pH in the complete-mix activated sludge (CMAS) process during 30 days of the operation were evaluated. There were not any differences between the mean concentration of MLSS in the case and control samples, however, the mean concentration of MLVSS in the case (1463.4 ± 419.2 mg/L) was more than the control samples (1244.1 ± 295.5 mg/L). Changes of the concentration of MLVSS over time, follow the first and second-order reaction with and without exposure of SMFs, respectively. However, the slope of the line in the case samples was 6.255 higher than the control samples. The mean of α-factor in the case samples increased by -0.001 higher than the control samples. Changes in pH in both groups of the reactors were not observed. 15 mT intensity of SMFs can promoted oxygen transfer to the biomass and, increase the concentration of MLVSS in the aeration reactor of CMAS. SMFs have a potential to be consider as an alternative method to stimulate the microbial growth rate in the aeration reactor and produce bioflocs with higher density in CMAS.
Distribution and prevalence of ixodid tick species (Acari: Ixodidae) infesting cattle from North-eastern Uganda [Karamoja]; 2019-2022
Authors: Etiang Patrick , Musoba Abubakar , Nalumenya David , Ndekezi Christian , Bbira Johnson , Ochwo Sylvester , Tweyongyere Robert , Muhanguzi Dennis 
Published: March 2023

Abstract
Background: Ticks and tick-borne diseases (TTBDs) are a significant threat to livestock production in sub-Saharan Africa. Transhumance pastoralism practiced in Karamoja region and other factors like cattle trade, communal grazing and the presence of wildlife predispose cattle to TTBDs. Tick species abundance and distribution data can be used as a tool for early disease diagnosis and inform tick control strategies. However, these data for north-eastern Uganda are currently limited; previous surveys were relatively localized and did not consider the effect of seasonality on tick abundance.
Methods: We randomly collected tick specimens from 1,534 cattle spread across Karamoja region in both the rainy and the dry seasons. The ticks were identified using morpho-taxonomic keys and the tick species identities confirmed by the 16S rRNA gene sequencing and phylogenetic analysis.
Results: A collection of 18,637 ticks was examined and 15 tick species from three genera (10 Rhipicephalus; 3 Amblyomma; 2 Hyaloma species) were identified. Rhipicephalus appendiculatus was the most dominant (37.9%) tick specie, followed by Amblyomma variegatum (32.3%); A. lepidum (17.3%); R. evertsi evertsi (7.8%); and R. decoloratus (1.4%). Eight of these tick species were ubiquitous in the study districts while six were found in isolated areas. The dry season was associated with a higher proportion of tick-infested cattle (91%) compared to the rainy season (89.8%); a difference that we did not find statistically significant (χ2 = 0.5077, n = 1385, p = 0.476). The overall cattle infestation rate was mainly dominated by 5 tick species namely: A. variegatum (55%), R. appendiculatus (53%), A. lepidum (41%), R. Evertsi (22%), and R. Decoloratus (8%). Napak and Amudat districts registered the highest (95.4% and 94.9%) and lowest (80.9% and 80.7%) cattle tick infestation rates during the rainy and dry seasons respectively. Rhipicephalus microplus was confirmed in Amudat, Kaabong and Napak districts.
Conclusion: This study demonstrates high cattle tick infestation rates by a battery of tick species in Karamoja region. We identified both R. microplus and R. decoloratus which indicates that R. microplus has recently been introduced in this region. This calls for effective tick control responses to prevent further spread of this invasive cattle tick specie.
miRNA-210 increased apoptosis of testicular tissues in cryptorchidism mice model through INHBB-Smad2/3 signaling pathway
Authors: Li Hu , Wang Gang , Wu Fei , He Ping , Chao Min , Zhang Yin 
Published: March 2023

Abstract

Background Cryptorchidism, as one of the common diseases of genitourinary abnormalities in newborn boys, has become an important factor leading to male infertility in the future. However, the specific pathogenesis remains poorly understood. The present experimental study aimed to clarify the mechanism of spermatogenic dysfunction caused by miR-210 in cryptorchidism. Methods In this study, 16 male ICR mice were classified into cryptorchidism group (n = 8), normal control group (n = 8), and the mice were killed in different age to create cryptorchidism self-control group. Then, the gene expression of miR-210 in testis tissues of three groups of mice, was detected by qPCR. HE staining and Tunel fluorescence staining were used to observe pathological changes and apoptosis of testis in cryptorchidism mice. The protein expression of INHBB was observed by immunohistochemistry. Finally, Western blot was used to detect the related proteins in the INHBB-Smad2/3-Casp3 pathway. Results The results indicated the expression of miR-210 was the most significant difference on the 14th day after cryptorchidism operation. We found that 14 days after the operation, apoptosis in the testis of cryptorchidism mice increased significantly. Finally, we found that the protein expressions of INHBB,Smad2/3, P-Smad2/3, and Caspase3 in the testicular tissues of cryptorchidism mice were significantly increased by detecting cryptorchidism mice with increased expression of miR-210. Conclusion Our results revealed the function of miR-210 and established the regulatory relationship between miR-210 and INHBB, which plays an important role in testicular tissue apoptosis.
Comparative Theoretical Study of Quinazolinone Derivatives in Different Solvents and Gas Phase. A Density Functional Theory (DFT) Calculations
Authors: Thapa Alina , Tamang Suveksha , Chettri Kaushilaya , Biswas Joydeep 
Published: March 2023

Abstract
In this work, five different Quinazolinone derivatives [C14H20(CO)2N2, C14H20(CO)2N2(2-Cl), C14H20(CO)2N2(4-Cl), C14H20(CO)2N2(4-OCH3) and C14H20(CO)2N2(4-NO2)] in gas and four different solvents (chloroform, acetone, methanol and DMSO) are theoretically studied and compared using DFT, B3LYP with basis set 6-31G(d,p). SCRF (self-consistent reaction field) for solvation and TD-DFT for UV/Vis spectra were also performed. With the increasing dielectric constant of a solvent, the effects like change in polarization, dipole moment, and charge delocalization are enhanced, resulting in the molecules' stability and reactivity determination due to the influence on the energy orbitals and other different parameters. Optimization, geometrical parameter determination, FMO, MEPs, ESP with TDA analysis, electronic energy, dipole moment, polarizability, different reactivity parameter calculation concerning HOMO-LUMO observations and UV/Vis spectroscopic characterization were obtained and analyzed.
Impact of selenium nanoparticle-enriched Lactobacilli feeding against Escherichia coli O157:H7 infection of BALB/c mice
Authors: Esfandiari Zahra , Vakili Bahareh , Ahangarzadeh Shahrzah , Esfahani Salar Nasr , Shoaei Parisa 
Published: March 2023

Abstract
The effectiveness of selenium nanoparticle (SeNP)-enriched Lactiplantibacillus plantarum and Lactobacillus acidophilus was studied against Shiga toxin-producing Escherichia coli O157:H7 infection on the intestinal fragments and kidney tissue of BALB/c mice.
Gut microbiota-targeted bacteria and E. coli O157:H7 counts were obtained by qPCR and PCR.
Histology of ileum, colon, and kidney tissues and Stxs secretions were analyzed until one-week post-infection.
Mice fed with SeNP Lpb. plantarum in the pre-infection feeding groups have lower E. coli O157:H7 counts, and lower intestinal damage than the infected group.
The lowest mean fecal probiotics counts were in the L. acidophilus group (7.61 log 10). In pretreatment groups of SeNP L.acidophilus and L.acidophilus, the mean counts of bacteria decreased to 104 CFU/g by day 7. The lowest stx copy number was demonstrated in SeNP Lpb. plantarum feeding groups’ day 7 (P < 0.05).
Feeding groups with SeNP Lpb.plantarum had significantly higher members of Lactobacilli in their fecal microbiota than the control group on day 7.
It was clarified that Se-enriched Lpb. Plantarum and L. acidophilus can be useful as a method of preventing STEC infections. The viability of STEC infections exposure to selenium-enriched Lactobacillus spp. was decreased more than for non Se enriched Lactobacillus spp.
‘Retro-uncinate Superior Mesenteric Vein’ – first report of a rare congenital anomaly and possible embryological explanation
Authors: Patel Prerak , Yelamanchi Raghav , Patkar Shraddha , Dhanwate Anant D , Bhandare Manish S , Shrikhande Shailesh V , Chaudhari Vikram A 
Published: March 2023

Abstract
We evaluated a 61-year-old female for a periampullary cancer. The preoperative contrast enhanced CT scan demonstrated an abnormal course of superior mesenteric vein (SMV). The vein was absent from its normal location in the groove behind neck of the pancreas and anterior to the superior mesenteric artery. It was found to be coursing anomalously, posterior to the uncinate process of pancreas and on the right of superior mesenteric artery. This is probably the first report of this anomaly. We had to coin the term ‘retro- uncinate SMV’ to describe this anatomical variation. Rest of the porta structures and pancreas were found to be anatomically normal in this patient. This is a rare anomaly, probably does not have a pathological consequence and remained unreported. Congenital variations in porta structures are relatively common. Careful and thorough preoperative assessment of radiology is essential to have a roadmap to avoid injuries to such anomalous structures during surgery. Preoperative identification of this anomaly resulted in a safe pancreaticoduodenectomy in this patient. Development of portal venous system and pancreas are closely related due to their anatomical proximity. Normally, the left part of ventral pancreatic anlage regresses and right part rotates posterior to the developing SMV. Malrotation of this right ventral pancreatic anlage anterior to the developing SMV or Regression of right ventral pancreatic anlage and persistence and development of left ventral pancreatic analge anterior to the developing SMV is the only logical explanation for development of this anomaly.
Brain tumor magnetic resonance images enhanced by hybrid method based on deep learning paradigm
Authors: Gül Mehmet , Kaya Yılmaz 
Published: March 2023

Abstract
The development of software engineering has given very successful results in the field of medical diagnosis in recent years. Deep learning and machine learning applications give remarkable results in the detection, monitoring, diagnosis, and treatment of possible tumoral regions with the analysis of the obtained medical images and data mining. Studies to diagnose brain tumors are essential because of the wide variety of brain tumors, the importance of the patient's survival time, and the brain tumor's aggressive nature. Brain tumors are defined as a disease with destructive and lethal features. Detection of a brain tumor is an essential process because of the difficulty in distinguishing between abnormal and normal tissues. With the right diagnosis, the patient can get excellent treatment, extending their lifespan. Despite all the research, there are still significant limitations in detecting tumor areas because of abnormal lesion distribution. It may be challenging to locate an area with very few tumor cells because areas with such small areas frequently appear healthy. Studies are becoming more common in which automated classification of early-stage brain tumors is performed using deep learning or machine learning approaches. This study proposes a hybrid deep learning model for the detection and early diagnosis of brain tumors via magnetic resonance imaging. The dataset images were subjected to Local Binary Pattern (LBP) and Long Short-Term Memory (LSTM) algorithms. The highest accuracy rate obtained in the hybrid model created is 98.66%.
Effect of epoxidized Cassia fistula seed oil (ECFSO) on poly(vinyl chloride) films in the presence of stabilizers
Authors: Rajput Chetan V. , Mukherjee Rupa B. , Sastry Nandhibatla V. , Chikhaliya Navin P. 
Published: March 2023

Abstract
The authors have requested that this preprint be removed from Research Square.
Angle constrained Paths
Authors: Asaeedi Saeed , Shamaee Mahsa Soheil 
Published: March 2023

Abstract
The traveling salesman problem (TSP) is a well-known and most widely studied problem in combinatorial optimization. The Dubins TSP is a variant of TSP for curvature-constrained vehicles. In this paper, another variant of TSP, called angle-constrained TSP, is considered for vehicles that can either move straight or turn, i.e., no voluntary curve is possible. The vehicles are only allowed to make on-the-spot turns and straight motions. We impose turn angle constraint on vehicles. Hence, some additional arbitrary points, called stop points, are added to reach an unreachable target. Here, computing the minimum number of stop points walking on the optimal TSP path is presented as the solution of angle-constrained TSP. In addition, we consider two other problems: computing an angle-constrained path on the vertices with the minimum number of stop points and computing an angle-constrained path with the minimum perimeter. In both problems, there is no need to stay on the optimal TSP path. Here, some algorithms are developed to solve these three problems and are examined on some datasets. These algorithms can be used for the path planning of robots with turn angle constraints.
Projected 2023 cost reduction from TNF-alpha inhibitor biosimilars in dermatology: a national Medicare analysis
Authors: Roster Katie , Gronbeck Christian , Feng Hao 
Published: March 2023

Abstract
The introduction of biosimilars for adalimumab and etanercept may yield a reduction in healthcare costs associated with biologics. With the influx of biosimilars into dermatology, it is important to recognize how utilization among dermatologists may impact costs. In this study, we referenced recent pricing and utilization trends for existing biosimilars and prescribing patterns for branded adalimumab (Humira) and etanercept (Enbrel) to estimate how utilization of biosimilars in dermatology may reduce healthcare costs. The findings suggest that these biosimilars could reduce Medicare Part D spending on biologics by $57–131 million in 2023, depending on utilization and pricing.
The Beurling theorem in space-time algebras
Authors: Tyr Othman , Daher Radouan 
Published: March 2023

Abstract
In this work, the space-time Fourier transform (SFT) introduced by E. Hitzer, satisfies some uncertainty principles of the algebra for space-time Cl(3,1)-valued signals over the space-time vector space R(3,1). An analog of the Beurling theorem for the (SFT) is obtained. As a straightforward consequence of Beurling's theorem, other versions of the uncertainty principle, such as the Hardy, Gelfand-Shilov, Cowling-Price and Morgan theorems are also deduced.
Exploring the Influencing Factors on Breast Self-Examination among Ethiopian Women, 2022: A Qualitative Study
Authors: Hussein Dursa , Gashaw Ketema , Geleta Tinsae Abeya , Girma Derara , Geleta Leta Adugna , Oyato Befekadu Tesfaye 
Published: March 2023

Abstract
Background: Breast cancer (BC) is the leading cause of cancer death worldwide and the second most common cancer overall. Breast self-examination (BSE) is one of the cheapest methods used for the early detection of breast cancer in asymptomatic women. However, in Ethiopia, most of thebreast self-examination studies were not conducted among women in the general population. Therefore, this study aimed to assess breast self-examination practice and associated factors among women of reproductive age in the North Shewa Zone, Oromia.
Method: A community-based cross-sectional qualitative study design was conducted from May 1, 2022 to June 30, 2022. Five focus group discussions (FGDs) were conducted with 46 women from four selected districts in North Shewa Zone, Oromia. A Purposive sampling technique was used to select participants for FGD
Results: Four themes emerged as the results of the in- focus group discussions, namely knowledge of breast cancer, knowledge regarding breast self-examination, Perceived benefits of treatment, barriers to performing regular BSE and enablers of BSE practice.
Conclusion: The study identified and described the factors influencing BSE practice which are inadequate knowledge regarding BSE practice and BC and also, misconceptions of BC and BSE practice. So, BSE shall be encouraged by improving knowledge of BSE practice and perception of BSE practice and BC through providing special health education for women at all levels. To overcome this problem, effort is needed from health system management like, North Shewa zonal Health Office, and the regional health bureau, Health care providers, cancer associations at the regional health bureau.
Asexual reproduction of Aurelia aurita depends on the presence of a balanced microbiome at polyp stage
Authors: Jensen Nadin , Weiland-Bräuer Nancy , Joel Shindhuja , Chibani Cynthia Maria , Schmitz Ruth Anne 
Published: March 2023

Abstract

The Cnidarian Aurelia aurita undergoes a complex life cycle alternating between the benthic polyp to the pelagic medusa. In absence of its natural microbiome, the asexual reproduction of this jellyfish is severely comprised, with limited strobilation and ephyrae release, whereas the recolonization of sterile polyps can restore this defect. Yet, the timing and molecular consequences of the microbiome during the strobilation process had not been investigated. Here, we demonstrate that a microbiota must be present at the polyp stage, before strobilation is initiated, to ensure normal asexual reproduction and a successful polyp-to-jellyfish transition. Addition of a microbiota at later stages of sterile animals failed to restore the defected process. Absence of a microbiome was accompanied with reduced transcription of developmental and strobilation genes. The transcription of strobilation genes was exclusively observed for native and recolonized polyps, pointing to the microbiome's impact on the strobilation process at the molecular level. Moreover, direct cell contact between the host and the bacteria was required for the generation of normal offspring. Overall, this study demonstrates that the timing of the host-microbe interaction in this developmental animal model is crucial, with the microbial impact being essential at the polyp stage, to ensure normal polyp-to-jellyfish transition.
WITHDRAWN: Physical and Mathematical Consistency of the Twin Cosmological Model
Authors: ZEJLI Hasnae , ZEJLI Hicham 
Published: March 2023

Abstract

The Janus Cosmological Model is based on a system of two coupled field equations. It explains the nature of dark matter and dark energy with negative mass and without the runaway paradox that arises in general relativity. We first recall how this system was built, from a simple Newtonian toy model to a relativistic bimetric theory, that is now improved in order to fulfill mathematical constraints and set up on a Lagrangian derivation.
Interception of Summer and Winter Insolation by Five Shade Tree Species
Authors: Gardner Timothy J. , Sydnor T. Davis 
Published: March 2023

Abstract
Five species of ornamental trees were examined with an Eppley pyranometer for interception of total solar radiation between 380 nm and 1100 nm. Measurements were made at the northern dripline and expressed as percentage of shade. Trees were selected for varying forms, branch, and canopy densities. Mean percentage of shade for the fully foliaged and leafless canopies, respectively, were Pyrus calleryana ‘Chanticleer’ 75% and 43%, Acer rubrum ‘Red Sunset’ 69% and 25%, Zelkova serrata ‘Village Green’ 61% and 24%, Gymnocladus dioicus 60% and 15%, Gleditsia triacanthos inermis ‘Moraine’ 56% and 21%. No statistically significant (PSO.05) correlation was observed among solar radiation intercepted and photosynthetically active radiation (PAR) intercepted, pathlength, silhouette area, and canopy volume. Growth habit did not affect shading capacities significantly.
Genetic and Environmental Effects on Mean Maturity Date and Uniformity in Broccoli
Authors: Hulbert S. H. , Orton T. J. 
Published: March 2023

Abstract
Fourteen hybrid progenies from a diallel cross of 6 commercial broccoli (Brassica oleracea var. italica) inbreds were grown at 3 locations on 3 planting dates. The hybrids were compared for uniformity and for the average number of days to maturity. The earliness of the hybrids was predicted from the performance of the parents while their uniformity was not. Genotype × environment interactions accounted for 44.4% of the total variation in plot uniformity and indicated that replication over planting dates and locations is valuable when screening for stable and uniformly maturing hybrids.
New Mineral Names: Arsenic and Lead
Authors: Celestian Aaron J. 
Published: March 2023

In this issueThis issue of New Mineral Names provides a summary of new species that contain arsenic and lead. As of November 2022, there are 1219 minerals that contain constituent arsenic or lead, which is roughly 20% of all known mineral species. These two elements are an important component in many of the newly described minerals that typically form from hydrothermal or other diagenetic processes. Here we look at nitroplumbite, thorasphite, tennantite-(Cd), paradimorphite, tombstoneite, aldomarinoite, lomardoite, dobšináite, panskyite, yugensonite, and kufahrite.