bluuebunny/crossref_metadata_2025_split huggingface dataset:
De novo assembly and annotation of Hellula undalis (Fabr.) (Lepidoptera: Pyralidae) transcriptome
Authors: Prajapati Malyaj R , Kumar Pankaj , Shanker Ravi , Singh Reetesh Pratap , Singh Rajendra , Bharti Mahesh Kumar , Singh Jitender , Kapoor Neelesh , Gangwar L. K. , Gaurav S. S. , Dixit Rekha , Prakash Satya 
Published: July 2023

Abstract
Background
The cabbage webworm, Hellula undalis (Fabricius) (Lepidoptera: Pyralidae), is a significant pest of brassicas and other cruciferous plants in warm regions worldwide. Transcriptome analysis is valuable for investigation of molecular mechanisms underlying the insect development and reproduction. De novo assembly is particularly useful for acquiring complete transcriptome information of insect species when there is no reference genome available. In case of Hellula undalis, only 17 nucleotide records are currently available throughout NCBI nucleotide database. Genes associated with metabolic processes, general development, reproduction, defense and functional genomics were not previously predicted in the Hellula undalis at the genomic level.
Methods & Results
To address this issue, we constructed Hellula undalis transcriptome using Illumina NovaSeq6000 technology. Approximately 48 million 125 bp paired-end reads were obtained from sequencing. A total of 30,451 contigs were generated by de novo assembly of sample and were compared with the sequences in the NCBI non-redundant protein database (Nr). In total, 71% of contigs were matched to known proteins in public databases including Nr, Gene Ontology (GO), and Cluster Orthologous Gene Database (COG), and then, contigs were mapped to 123 via functional annotation against the Kyoto Encyclopedia of Genes and Genomes pathway database (KEGG). In addition, we compared the ortholog gene family of the Hullula undalis, transcriptome to Spodoptera frugiperda, spodotera litura and spodoptera littoralis and found that 2,749 orthologous gene families are specific to Hullula undalis.
Conclusions
This study is the first transcriptome data for Hullula undalis. Additionally, it serves as a valuable resource for identifying target genes and developing effective and environmentally friendly strategies for pest control.
Beyond Spectral Measures: Multi-Scale Entropy Assessment of MEG Signals in Schizophrenia
Authors: Wolfson Erez J. , Fekete Tomer , Loewenstein Yonatan , Shriki Oren 
Published: July 2023

Abstract
Schizophrenia is a severe disruption in cognition and emotion, affecting fundamental human functions. In this study, we applied Multi-Scale Entropy (MSE) analysis to resting-state MEG data from 54 schizophrenia patients and 98 healthy controls. This method quantifies the temporal complexity of the signal across different time scales using the concept of sample entropy. Results show significantly higher sample entropy in schizophrenia patients, primarily in central, parietal, and occipital lobes, peaking at time scales equivalent to frequencies between 15 and 24 Hz. To disentangle the contributions of the amplitude and phase components, we applied the same analysis to a phase-shuffled surrogate signal. The analysis revealed that most differences originate from the amplitude component in the δ, α, and β power bands. While the phase component had a smaller magnitude, closer examination reveals clear spatial patterns and significant differences across specific brain regions. We assessed the potential of MSE as a schizophrenia biomarker by comparing its classification performance to conventional spectral analysis and a cognitive task (the n-back paradigm). The discriminative power of MSE and spectral features was similar, with a slight advantage for MSE features. The results of the n-back test were slightly below those obtained from MSE and spectral features.
Qualitative and quantitative reservoir characterization using seismic inversion based on particle swarm optimization and genetic algorithm: A comparative case study
Authors: Kant Ravi , Maurya S.P. , Singh K.H. 
Published: July 2023

Abstract

Accurate reservoir characterization is necessary to effectively monitor, manage, and increase production. A seismic inversion methodology using a genetic algorithm (GA) and particle swarm optimization (PSO) technique has been devised in thisstudy to characterize the reservoir both qualitatively and quantitatively. It has always been difficult and expensive to disclose deeper reservoirs in exploratory operations when using traditional approaches for reservoir characterization hence inversion based on advanced technique (based on GA and PSO) is proposed in this study. The main goal is to use GA and PSO to significantly lower the fitness (error) function between real seismic data and modeled synthetic data, which will allow us to estimate subsurface properties and, ultimately, accurately characterize the reservoir. Using two synthetic data and one real data from the Blackfoot field in Canada, the study examined subsurface acoustic impedance and porosity in the inter-well zone. Porosity and acoustic impedance are layer features, but seismic data is an interface property, hence these characteristics provide more useful and applicable reservoir information. The inverted results aid in the understanding of seismic data by providing incredibly high-resolution images of the subsurface. Both the GA and the PSO algorithms deliver outstanding results for both simulated and real data. The inverted section accurately anticipated a high porosity zone (> 20%) that supported thehigh seismic amplitude anomaly by having a low acoustic impedance (6000-8500m/s∗ g/cc). This unusual zone is categorized as a reservoir (sand channel) and is located in the 1040–1065 ms time range. In this inversion process, after 400 iterations, thefitness error falls from 1 to 0.88 using GA optimization, compared to 1 to 0.25 using PSO. The convergence time for GA is 670680 seconds, but the convergence time for PSO optimization is 356400 seconds, showing that the former requires 88% more time than the latter. The study recommends PSO application over GA in any E & P project because PSO inversion delivers faster convergence times, excellent error minimization, and high-resolution subsurface data compared to inversion utilizing GA optimization.
Meckel-Gruber syndrome together with Dandy–Walker malformation; an atypical case report of a 2nd recurrence in a consanguine marriage
Authors: Alsamal Mohamad S. , Zitoun Osama A. , Abdulghani Ebtsam Abdulaziz , Sula Idris 
Published: July 2023

Abstract
Meckel-Gruber syndrome is a lethal disorder characterized by occipital encephalocele, polycystic kidneys, and polydactyly. In most cases, it is identified and terminated antenatally. In this report, the authors present a case of Meckel-Gruber syndrome together with Dandy-Walker malformation. A pregnant woman referred at the 28th week of gestation with an abnormal ultrasound scan showing posterior encephalocele and bilaterally enlarged kidneys. Further imaging also indicated communication between the 4th ventricle and posterior cerebellar cerebrospinal fluid space, after which the fetus was diagnosed with Meckel Gruber syndrome and Dandy–Walker malformation. Pregnancy termination was refused by the parents and the offspring was prematurely born to be the 2nd recurrence of Meckel Gruber syndrome in this consanguine family. Remarkably, at the 3 different pregnancies, ultrasound was inconclusive before the 7th month of gestation. Though up to date Meckel-Gruber syndrome is ultimately lethal, the lifespan of affected newborns varied greatly. We suggest developing a severity classification to estimate life expectancy in unterminated cases.
All Silicon MIR super absorber using fractal metasurfaces
Authors: Ali Alaa M. , Ghanim AbdelRahman M. , Othman Muhammad , Swillam Mohamed A. 
Published: July 2023

Abstract
The study of blackbody radiation led to the development of quantum mechanics more than a century ago. A blackbody is an ideal absorber, as it absorbs all the electromagnetic radiation that illuminates it. No radiation is transmitted through it, and none is reflected. Now, "bodies" with high absorption qualities are crucial in numerous scientific and technological fields. Perfect absorbers can be used as photodetectors, thermal images, microbolometers, and thermal photovoltaic solar energy conversion. The spectrum of Mid-infrared (MIR) wavelengths offers numerous advantages for a wide range of applications. Among these applications is chemical and biological detection. In this study, we propose a fractal broadband silicon (Si) absorber. The proposed structure is composed of three layers: metal, dielectric, and metal (MDM), with the metal being n-type D-Si and the dielectric being Silicon Carbide (SiC). The structural composition displays a broad absorption profile across a broad spectrum of infrared wavelengths, ranging from 3 to 9 µm. The architectural design was derived from the Sierpinski carpet fractal, and different building locks were simulated to attain optimal absorption. Silicon that has been doped exhibits superior performance compared to metals in energy harvesting applications that utilize plasmonics at the mid-infrared range. Typically, semiconductors exhibit rough surfaces than noble metals, resulting in lower scattering losses. Moreover, silicon presents various advantages, including compatibility with complementary metal-oxide-semiconductor (CMOS) and simple manufacturing through conventional silicon fabrication methods. In addition, the utilization of doped silicon material in the mid-IR region facilitates the creation of microscale integrated plasmonic devices. This combination enables the production of numerous traditional plasmonic devices. The 2D finite element method (FEM) approach via COMSOL software is used to obtain the numerical results. The suggested fractal absorber exhibits high absorption enhancement in the Mid-IR range.
Costs associated with adverse drug reactions in an older population admitted to hospital: A prospective cohort study
Authors: Bennett Kathleen , Cahir Caitriona , Sorensen Jan 
Published: July 2023

Abstract
Purpose
This study examines healthcare costs associated with adverse drug reactions (ADR) in an older population admitted acutely to an Irish tertiary hospital.
Methods
This is a prospective cohort study involving older persons admitted to hospital with and without an ADR. Data was collected at baseline during hospitalisation and after hospital discharge. Participants provided information on healthcare resource use three months before and after discharge. For each healthcare resource, unit costs were derived and applied. Average, accumulated costs of hospitalisation and related health and social care were calculated. The average costs associated with ADR and non-ADR hospitalisation and post-discharge care were compared using difference-in-difference analysis and presented with 95% confidence intervals (CI). Costs by preventability and severity of ADR are also presented.
Results
A total of n=230 participants were included (n=93 associated with ADR and n=137 without ADR). The average cost associated with an ADR was estimated at €2047 (95% CI: -€889 to €4983). The mean incremental cost of definite preventable ADRs was estimated at €1648 (95% CI: -€4310 to €7605), possible preventable ADRs €2259 (95 CI: -€1194 to €5712) and unavoidable ADRs €1757 (95% CI: -€3377 to €6890). The mean incremental cost associated with moderate severe ADRs was estimated at €1922 (95% CI: -€1088 to €4932) and €3580 (95% CI: -€4898 to €12058) for severe ADRs.
Conclusion
ADRs leading to hospital admission are associated with modest incremental healthcare costs during and three months after admission. Severe and possibly preventable ADRs were associated with higher costs.
Intestinal Microbiome and Maternal Mental Health: Preventing Parenting Stress and Enhancing Resilience in Mothers
Authors: Matsunaga Michiko , Takeuchi Mariko , Watanabe Satoshi , Takeda Aya , Kikusui Takefumi , Mogi Kazutaka , Nagasawa Miho , Hagihara Keisuke , Myowa Masako 
Published: July 2023

Abstract
The number of mothers suffering from mental illness is increasing steadily, particularly under conditions of the coronavirus disease pandemic. The identification of factors that contribute to resilience in mothers is urgently needed to decrease the risks of poor physical and psychological functioning. We focused on the risk of parenting stress and psychological resilience in nonclinical mothers and conducted two studies to examine the relationships between intestinal microbiota, physical condition, and psychological state. Our results showed that alpha diversity and beta diversity of the microbiome are related to high parenting stress risk. Psychological resilience and physical conditions were associated with relative abundances of the genera Blautia, Clostridium, and Eggerthella. This study helps further understand the gut–brain axis mechanisms and supports proposals for enhancing resilience in mothers.
National prevalence and risk factors for tungiasis in Kenya
Authors: Elson Lynne , Kamau Christopher , Koech Sammy , Muthama Christopher , Gachomba George , Sinoti Erastus , Chondo Elwyn , Mburu Eliud , Wakio Miriam , Lore Jimmy , Maia Marta , Adetifa Ifedayo , Orindi Benedict , Bejon Phillip , Fillinger Ulrike 
Published: July 2023

Abstract
Background
Tungiasis is a highly neglected tropical skin disease caused by the sand flea, Tunga penetrans, the female of which burrows into the skin, causing pain and discomfort. The disease occurs throughout South America and Sub-Saharan Africa but there are few systematic data on national disease burdens.
Methods
We conducted the first nationally representative survey of tungiasis, including nine counties covering the major ecological zones of Kenya. A stratified multistage random sampling was used to select 22 primary schools from each of the nine counties and to select up to 114 pupils aged 8 to 14 years in each school. Pupils were examined thoroughly for tungiasis. Two surveys were conducted, the first between May and July 2021 and the second between October 2021 and April 2023.
Results
The overall prevalence of tungiasis in the first survey was 1.3% (95% CI 1.1–1.5%), and 0.89% in the second survey. The prevalence ranged from 0.08% (95% CI 0.01–0.6%) in Taita Taveta county to 3.2% (95% CI 2.4–4.4%) in Kajiado county. Tungiasis infection was associated with county of residence, male sex (adjusted Odds ratio (aOR) 2.01 95% CI 1.52–2.67), and lower age (aOR 0.81 95% CI 0.75–0.88). For the first time we demonstrate an association with attending public schools (aOR 5.62 95% CI 1.20–26.22) and lower socioeconomic status (aOR 0.10 95% CI 0.03–0.33).
Conclusion
Tungiasis is widely but heterogeneously distributed across Kenya. School-based surveys offer an efficient strategy for mapping tungiasis distribution.
Early to Bed and Early to Rise Makes a Man Healthy, Wealthy and Wise: The Relationship of Sleep to Health, Income and Education
Authors: Berry-Cabán Cristóbal S. , Beltran Thomas , McKiernan Sharon P. , Han Rachel H. , Choi Y. Sammy 
Published: July 2023

Abstract

Purpose To provide evidence for Benjamin Franklin's proverb, “Early to Bed and Early to Rise Makes a Man Healthy, Wealthy and Wise,” we used survey data to evaluate the relationship between sleep duration and health, income, and education.Methods Sleep, demographic, and wellness data from 49,365 participants aged 18 years and older was sourced from the 2017 Behavioral Risk Factor Surveillance System (BRFSS). The BRFSS is a cross-sectional telephone survey conducted by state health departments with technical and methodological assistance provided by the Centers for Disease Control and Prevention. Self-reported sleep duration was assessed as well as self-perceptions of health and wellness. Participants’ income and educational level were used as representatives for ‘wealth’ and ‘wise,’ respectively.Results Individuals with average daily sleep durations between 7 and 9 hours reported significantly better physical and mental wellness as compared to those who reported fewer than 7 hours or more than 9 hours of sleep per day (p < 0.001). Similarly, these individuals reported the highest self-ratings of overall health and life satisfaction (p < 0.001), had the greatest percentage with 4-year college degrees and reported the highest levels of income of the three groups.Conclusion When using sleep duration as a proxy for ‘early to bed and early to rise,’ this study supports Benjamin Franklin's aphorism that adequate sleep “makes a man healthy, wealthy and wise.” Whether adequate sleep resulted in, or was a result of, better health, higher income and higher education, a relationship between them is clear.
Overexpression of TMEM79 combined with SMG5 is related to prognosis, tumor immune infiltration and drug sensitivity in hepatocellular carcinoma
Authors: Wang Yu , Jin Qin , Zhang Shu , Wang Yan 
Published: July 2023

Abstract
Background
Hepatocellular carcinoma (HCC) is a primary liver malignancy that is now relatively common worldwide. TMEM79 (Transmembrane Protein 79) is a Protein Coding gene. It has been reported to play diagnostic and prognostic markers in a variety of cancers and was found to be closely associated with immune infiltration in kinds of tumors in a follow-up study. One study found that Multiple nonsense-mediated mRNA processes require the involvement of SMG5[1]. SMG5 is associated with immune cell infiltration in HCC[2]. However, the relationship between TMEM79 expression in HCC and prognosis, its role and mechanism of action, and its relationship with SMG5 have not been studied. This article focuses on not only the prognostic role of TMEM79 and its biological significance including immuno-infiltration, tumor mutations and drug sensitivity, but also the interaction with SMG5 in HCC.
Methods
Differential expression analysis and the multiCox proportional hazards regression analyses of TMEM79 and SMG5 were performed by multiple databases. And then IHC was used to validate the differential expressions, correlation of TMEM79 and SMG5, and prognosis of TMEM79 and SMG5 in HCC in our research. Subsequently, we used R software to analyze the clinical phenotype of both: analysis of clinicopathological features, enrichment analysis, analysis of immune infiltration, analysis of immune checkpoints, analysis of drug sensitivity, and immunotherapy. Then TMEM79-related molecules were classified into two types by using R software. And then prognostic analysis, enrichment analysis, and immuno-infiltration analysis were performed for the two types.
Results
Both the database studies and the results of our research group showed that TMEM79 and SMG5 were differentially expressed in HCC and normal tissues. The multiCox proportional hazards regression analyses of TMEM79 and SMG5 showed that they were independent prognostic factors in HCC. Validation of immunohistochemistry showed that differential expression of TMEM79 and SMG5, which influenced the prognosis of patients with HCC, could be an independent prognostic factor. Results of the TCGA database study showed that TMEM79 and SMG5 were correlated with immune infiltration, immune checkpoints, drug sensitivity, and immunotherapy. We typed TMEM79-related molecules in HCC according to R software. Two types of TMEM79 correlated with clinical features, survival of patients with HCC, and immune infiltration.
Conclusion
TMEM79 are highly expressed in HCC and play an important role in the prognosis of patients with HCC. TMEM79 and SMG5 are positively correlated and may both associated with immune infiltration, and closely linked to immune checkpoints, drug sensitivity, and immunotherapy in HCC.
Phase-dependent activity-stability relationships of molybdenum sulfide hydrogen evolution electrocatalysts
Authors: Escalera-López Daniel , Iffelsberger Christian , Zlatar Matej , Maselj Nik , Pham Chuyen Van , Jovanovič Primož , Hodnik Nejc , Thiele Simon , Pumera Martin , Cherevko Serhiy 
Published: July 2023

Abstract

Molybdenum disulfide (MoS2) is widely regarded as a competitive hydrogen evolution reaction (HER) catalyst to replace platinum in proton exchange membrane water electrolysers (PEMWEs). Despite the extensive knowledge of its HER activity, stability insights under HER operation are scarce. This is paramount to ensure long-term operation of Pt-free PEMWEs, and gain full understanding on the electrocatalytically-induced processes responsible for HER active site generation. The latter are highly dependent on the MoS2 allotropic phase, and still under debate. We rigorously assess these by simultaneously monitoring Mo and S dissolution products using a dedicated scanning flow cell coupled with downstream analytics (ICP-MS), besides an electrochemical mass spectrometry setup for volatile species analysis. We observe that MoS2 stability is phase-dependent: lamellar-like MoS2 is highly unstable under open circuit conditions, whereas cluster-like amorphous MoS3 − x instability is induced by S loss and undercoordinated Mo site generation. Guidelines to operate non-noble PEMWEs are therefore provided based on the stability number metrics, and an HER mechanism which accounts for Mo and S dissolution pathways is proposed.
HPV E6/E7 mRNA detection in women with abnormal histology in Qazvin province, Iran
Authors: Benvari Sepideh , Aslanimehr Masoumeh , Samiee-Rad Fatemeh , Naserpour-Farivar Taghi 
Published: July 2023

Abstract
Objective: Human papillomavirus (HPV) infection, especially with type 16 or 18, is associated with cervical cancer. Two HPV proteins, E6 and E7, are essential factors in the carcinogenesis of HPV. The purpose of this study was to evaluate the presence of HPV 16/18 E6/E7 mRNA in women with abnormal histology in Qazvin province, Iran.
Results: HPV 16/18 DNA and E6/E7 mRNA was observed in 60.6% and 42.4% of samples, respectively. The frequency of positive HPV 16/18 and E6/E7 results increased with the severity of the injury, but the latter correlates better with progressive lesions. Despite the non-different sensitivity, PPV, and NPV of these two tests, E6/E7 mRNA showed higher specificity for detecting high-grade lesions (52.30% vs 75.38, P = 0.006).
Severe familial coronary artery spasm in two siblings : about 2 cases
Authors: Kerrouani Oualid , Faraj Raid , Bouchaala Abderrahmane , Dib Hassan , Lahmouch Nouhaila , Bargach Iness , Kihoul Ouassima , Zarzur Jamila , Cherti Mohamed 
Published: July 2023

Abstract
Background: Vasospastic angina (VSA) is the spasm of coronary arteries causing transient myocardial ischemia. VSA is commonly managed with antispasmodic medications including calcium-channel blockers (CCB) and nitrates. When vasospasm is refractory to conventional medications, unconventional treatment modalities may be used for symptomatic relief. Case presentation: Coronary artery spasm (CAS) was observed in two sisters. Neither of them had significant atheromatous stenosis in the coronary arteries. The 22-year- younger sister presented with rest angina in the early morning. The 32-year-old elder sister complained of rest and effort angina. Their coronary angiogram showed spontaneous spasm in the proximal segment of the left anterior descending coronary artery (LAD). The youngest one had resistant and recurrent coronary vasospasm involving different segments of the coronary tree causing myocardial infarction with total occlusion of the proximal segment in the left anterior descending coronary artery. Our patients presented with a lesser-known phenomenon called refractory VSA, where intermittent vasospasm continues despite being on a combination of two medications. Conclusions: The familial appearance of coronary artery spasm had been previously reported. Although it is not well understood, the underlying mechanism appears to involve a combination of endothelial damage and vasoactive mediators. Genetic factors such as human leucocyte antigen (HLA) contributes to susceptibility to coronary spasm in some patients with VSA. Treatment for VSA is well-documented, however, little data is available for refractory VSA.