bluuebunny/crossref_metadata_2025_split huggingface dataset:
Multipotent ubiquitin/ubiquitin-like deconjugation activity of therhizobialeffector NopD
Authors: Li Ying , Perez-Gil Jordi , Lois Maria , Varejão Nathalia , Reverter David 
Published: September 2023

AbstractPost-translational modification of proteins by ubiquitin-like modifiers (UbL), such as SUMO, ubiquitin or Nedd8, contribute to regulate most pathways in the cell. Protein modification can be reversed by dedicated UbL deconjugating proteases families. During bacterial infection, a repertoire of effector proteins, including deconjugating proteases, are released to perturb the host cell defense to favor bacterial survival. NopD, an effector protein from rhizobia involved in nodule symbiosis in legumes, possesses deSUMOylation, and unexpectedly, deubiquitination and deNeddylation activities. Here we show two crystal structures ofBradyrhizobiumNopD in complex with eitherArabidopsisSUMO2 and ubiquitin at 1.50 or 1.94 Å, respectively. Despite their low sequence identity, SUMO and ubiquitin interact with a similar NopD interface by means of a unique loop insertion in the NopD sequence. Biochemical and infiltrations in tobacco leaves reveal specific residues that discriminate between deubiquitination and deSUMOylation. These unusual multiple deconjugating activities against SUMO, ubiquitin and Nedd8, represent a paradigmatic example of an optimized protease to perturb distinct UbL post-translational modifications during host cell infection.Significance StatementDuring bacterial infection, includingrhizobiasymbiosis in legume plants to fix atmospheric nitrogen, a set of effector proteins, such as ubiquitin/ubiquitin-like deconjugating proteases, are released to perturb the host cell defense to favor bacterial survival. We have discovered that therhizobialeffector protein, NopD, encompasses triple deconjugation activities against SUMO, ubiquitin and Nedd8. Structural analysis of NopD in complex with SUMO and ubiquitin reveals the presence of a loop insertion in the protease-substrate interface to allow this multiple substrate binding capability. Such unusual deconjugating activities in NopD for ubiquitin, SUMO and Nedd8 modifiers, represent a paradigmatic example of an optimized protease domain to perturb distinct UbL post-translational modifications during host cell infection.
Leishmania PNUTS discriminates between PP1 catalytic subunits through a RVxF-ΦΦ-F motif and polymorphisms in the PP1 C-tail and catalytic domain
Authors: Zhang Yang , Sabatini Robert 
Published: September 2023

AbstractPP1 phosphatases lack substrate specificity and associate with specific regulatory subunits to achieve selectivity. Among the eight PP1 isotypes in Leishmania, PP1-8e associates with the regulatory protein PNUTS along with the structural factors JBP3 and Wdr82 in the PJW/PP1 complex that modulates RNA polymerase II (Pol II) phosphorylation and transcription termination. Little is known regarding interactions involved in PJW/PP1 complex formation, including how PP1-8e is the selective isotype associated with PNUTS. Here, we show that PNUTS uses an established RVxF-ΦΦ-F motif to bind the PP1 catalytic domain with similar interfacial interactions as mammalian PP1- PNUTS and non-canonical motifs. These atypical interactions involve residues within the PP1-8e catalytic domain and N- and C-terminus for isoform specific regulator binding. This work advances our understanding of PP1 isoform selectivity and reveals key roles of PP1 residues in regulator binding. We also explore the role of PNUTS as a scaffold protein for the complex by identifying the C-terminal region involved in binding JBP3 and Wdr82, and impact of PNUTS on the stability of complex components and function in Pol II transcriptionin vivo. Taken together, these studies provide a potential mechanism where multiple motifs within PNUTS are used combinatorially to tune binding affinity to PP1, and the C-termini for independent binding of JBP3 and Wdr82, in the Leishmania PJW/PP1 complex. Overall, our data provide insights in the formation of the PJW/PP1 complex involved in regulating Pol II transcription in divergent protozoans where little is understood.
Analysis of frontier digital technologies in continuing airworthiness management frameworks and applications
Authors: Raoofi Tahmineh , Yasar Sahin 
Published: September 2023

Purpose
This study aims to elaborate on the existing link between maintenance practices and the digital world while also highlighting any unaddressed potential for digital transformation in aircraft maintenance. Additionally, explore how digital technologies contribute to optimizing efficiency within the continuing airworthiness management (CAM) processes.

Design/methodology/approach
A literature review was performed to provide a precise review of the authority regulations on CAM processes and existing literature on digital transformation, including artificial intelligence, machine learning, neural network and big data in civil aircraft maintenance and continuing airworthiness processes. This method is used to organize, analyze and structure the body of literature to identify research gaps in the selected scope of the study.

Findings
The high position of digital technologies in preventive and predictive maintenance and the need for legislative development for using them in CAM are emphasized. Moreover, it is shown in which area of CAM scientific research has been performed regarding the application of frontier digital technologies. In addition, the gaps between maintenance practices and the digital world, along with the potential scopes of digital transformation which has not been well addressed, are identified. And finally, how digital technologies can effectively increase efficiency in CAM processes is discussed.

Originality/value
To the best of our knowledge, no study comprehensively determined the body of existing knowledge on the aspects of digitalization related to the field of continuing airworthiness management and aircraft maintenance. The results of this study provide a positive contribution to airlines, policymakers, manufacturers and maintenance organizations achieving additional benefits from the implementation of digital technologies in the CAM processes.
Analysis of chip formation mechanism in turning of AA 7075/SiCp MMCs
Authors: Bhushan Rajesh Kumar 
Published: September 2023

Purpose
The purpose of this paper is to examine the quality of the turned surface. The quality of the surface produced depends on the nature of the chips, which are produced while turning metal matrix composites. This quality is a function of the machining parameters, tool material, tool configuration and elements of the composites.

Design/methodology/approach
In this study, the turning of AA7075/15 wt.% SiC (particle size 20–40 µm) composites is investigated. Thirty experiments were conducted, and the chip-formation mechanism in turning AA7075/SiCp composites at various combinations of cutting speeds, feed and depth of cuts was studied.

Findings
It is observed from the response surface methodology-based experimentation that in turning of coarser reinforcement (particle size 20–40 µm) composites, total gross fracture occurs. This causes small slices of chips and a higher shear plane angle. The nature of chips produced at various combinations of cutting speeds, feed and depth of cuts is different. The chips generated were segmented, spiral in cylindrical form, connected C type, chips with saw tooth, curled chips, washer C type chips, half-curved segmented chips and small-radii segmented chips.

Originality/value
The novelty of this research is that, so far, very little work has been published on the detailed analysis of chips produced during turning of AA7075/15 wt.% SiC (particle size 20–40 µm) composites.
Social contagion of online physician choice: the infection and immunity mechanism
Authors: Li Jia , Ma Shengkang , Yen David C. , Ma Ling 
Published: September 2023

PurposeIn the digital age, the spread of online behavior and real-world information leads to social contagion. This study aims to investigate the contagion phenomenon of online physician choice and then discuss its potential influence on the sub-specialization process in the healthcare service industry. In specific, this study aims to propose the basic mechanism of infection and immunity as follows – exposure to antigen may lead to an immune response, and the success of the immune response may depend on the provision of appropriate immune signaling.Design/methodology/approachData collected from haodf.com including 4 disease types and 247 physicians from 2008 to 2015 were used to test the proposed hypotheses. Panel vector autoregression method was utilized to analyze the panel data.FindingsThe obtained result shows that social contagion of physician choice over disease type is salient on e-consultation platforms, indicating that physicians associated with/on haodf.com are concentrating on an even narrower type of disease. Disclosing more simple signals (physician history orders) results in more disease concentration for that physician in the future. In contrast, disclosing more detailed signals (physician-contributed knowledge or physician reviews) leads to less disease concentration.Originality/valueThis finding implies that physician-contributed knowledge and physician reviews may act as immune signal which will tend to trigger a success immune response. This study not only suggests managers should be careful about the double-edged sword effect of online physician choice contagion but also provides the useful approaches to promote or restrain such a contagion in a flexible way.
Enhancing climate change adaptation governance through transforming institutions in Kwa-Zulu Natal Province, South Africa
Authors: Adom Richard Kwame , Simatele Mulala Danny , Das Dillip Kumar , Mukalazi Kalumba Ahmed , Sonwabo Mazinyo , Mudau Lindelani , Sithole Mikateko , Kubanza Serge , Vogel Coleen , Zhou Leocadia 
Published: September 2023

Purpose
Globally, climate change governance continues to be a significant challenge to policymakers, environmentalists and politicians despite international summits, conferences and programmes designed to find sustainable solutions to the climate change crises. Climate change continues to be viewed primarily as a challenge for the future, whereas many leaders and administrators globally regard it as an environmental issue rather than a challenge that encompasses all aspects of life. In South Africa, these misleading perceptions of climate change continue to prevail both at national and local levels. The government and private organisations do not attach the required levels of urgency needed to address the climate change crisis. While numerous policies and institutions have been established to address these challenges, they lack financial backing, coordination and synergy that cut across the broad objectives of environmental, social and economic agendas. Additionally, weak, eroding trust and manipulating of institutions continue to hinder effective policy implementation and focus-driven governance. This paper aims to explore the structural and governance weaknesses of climate change administration in the KwaZulu-Natal province and South Africa in general.

Design/methodology/approach
This paper used extensive literature reviews and a triangulated approach to investigate the weaknesses of the current governance structure in the context of institutional and capacity constraints.

Findings
The findings uncovered that most institutions and organisations mandated to address climate change challenges operate in silos, lack required investment and capacity and have weak accountability mechanisms with a shallow understanding of climate change governance.

Originality/value
This paper recommends better coordination between national, provincial and local governments as well as the private sector towards climate change activities and capacity to ensure that climate change actions are effectively implemented.
Modeling and influence on effective thermal conductivity of woven fabrics based on structure parameters
Authors: Yang Yunchu , Wang Hengyu , Yan Hangyu , Ni Yunfeng , Li Jinyu 
Published: September 2023

PurposeThe heat transfer properties play significant roles in the thermal comfort of the clothing products. The purpose of this paper is to find the relationship between heat transfer properties and fabrics' structure, yarn properties and predict the effective thermal conductivity of single layer woven fabrics by a parametric mathematical model.Design/methodology/approachFirst, the weave unit was divided into four types of element regions, including yarn overlap regions, yarn crossing regions, yarn floating regions and pore regions. Second, the number and area proportion of each region were calculated respectively. Some formulas were created to calculate the effective thermal conductivity of each element region based on serial model, parallel model or series–parallel mixing model. Finally, according to the number and area proportion of each region in weave unit, the formulas were established to calculate the fabric overall effective thermal conductivity in thickness direction based on the parallel models.FindingsThe influences of yarn spacing, yarn width, fabric thickness, the compressing coefficients of air layers and weave type on the effective thermal conductivity were further discussed respectively. In this model, the relationships between the effective thermal conductivity and each parameter are some polynomial fitting curves with different orders. Weave type affects the change of effective thermal conductivity mainly through the numbers of different elements and their area ratios.Originality/valueIn this model, the formulas were created respectively to calculate the effective thermal conductivity of each element region and whole weave unit. The serial–parallel mixing characteristics of yarn and surrounding air are considered, as well as the compression coefficients of air layers. The results of this study can be further applied to the optimal design of mixture fabrics with different warp and filling yarn densities or different yarn thermal properties.
Effects of supply chain learning on green innovation and moderating role of green transformational leadership
Authors: Pham Tho , Pham Hai Thanh 
Published: September 2023

PurposeThis study examines the effect of supply chain (SC) learning (i.e. supplier and customer learnings) on green innovation (i.e. green product and process innovations) and investigates the moderating role of green transformational leadership in the SC learning-green innovation linkage in the construction industry.Design/methodology/approachData are gathered from construction firms in Vietnam by a questionnaire survey. Hypotheses of the study framework are tested by hierarchical regression analysis.FindingsBoth supplier and customer learnings have positive effects on green innovation (both green process and product innovations). Furthermore, green transformational leadership moderates the linkage between supplier learning and green innovation but does not moderate the linkage between customer learning and green innovation.Practical implicationsConstruction firms need to constantly develop capabilities of SC learning for promoting their green innovation.Originality/valueThe present study is one of the first attempts in construction that investigates the importance of SC learning to achieving green innovation as well as the role of green transformational leadership for strengthening the effect of green learning on green innovation.
Impact of gender diversity on firm performance: empirical evidence from India
Authors: Laskar Najul , Sahu Jagadish Prasad , Choudhury Khalada Sultana 
Published: September 2023

PurposeThe main purpose of the study is to investigate the impact of gender diversity both at the board and workforce level on firm performance (FP) in the Indian context.Design/methodology/approachThis study is based on annual data of 200 companies listed on Bombay Stock Exchange (BSE) for the period 2012–2019. The authors have used the fixed-effects (FE) regression and system generalized method of moments to estimate the impact of board gender diversity and workforce gender diversity (WGD) on FP. The authors have used Blau's Index (BI) and Shannon's Index (SI) to measure gender diversity. Further, the authors have used return on assets and Tobin's Q (TBQ) to measure FP.FindingsThe authors' panel regression results suggest that board gender diversity and WGD have a positive and statistically significant impact on FP. The authors' findings are robust across different methods of estimation and alternative measures of FP.Originality/valueThis paper examines the impact of gender diversity both at the board and workforce level on FP of 200 companies listed on BSE. The authors' study contributes to the literature that is sparse in the Indian context and provides new insights on the impact of board and WGD on FP. The findings have useful policy implications. To achieve better performance, it is imperative to appreciate gender diversity at the governance and workforce level in a fast-growing economy like India.
Quasiperiodic graph model of rubber elasticity in double-network gels undergoing mechanochemical coupling
Authors: Zhang Aying , Xing Ziyu , Lu Haibao 
Published: September 2023

Purpose
The purpose of this paper is to study the mechanochemical effect and self-growth mechanism of double-network (DN) gel and to provide a quasiperiodic model for rubber elasticity.

Design/methodology/approach
The chemical reaction kinetics is used to identify the mechanochemical transition probability of host brittle network and to explore the mechanical behavior of endosymbiont ductile network. A quasiperiodic model is proposed to characterize the cooperative coupling of host–endosymbiont networks using the Penrose tiling of a 2 × 2 matrix. Moreover, a free-energy model is formulated to explore the constitutive stress–strain relationship for the DN gel based on the rubber elasticity theory and Gent model.

Findings
In this study, a quasiperiodic graph model has been developed to describe the cooperative interaction between brittle and ductile networks, which undergo the mechanochemical coupling and mechanical stretching behaviors, respectively. The quasiperiodic Penrose tiling determines the mechanochemistry and self-growth effect of DNs.

Originality/value
It is expected to formulate a quasiperiodic graph model of host–guest interaction between two networks to explore the working principle of mechanical and self-growing behavior in DN hydrogels, undergoing complex mechanochemical effect. The effectiveness of the proposed model is verified using both finite element analysis and experimental results of DN gels reported in literature.
Effect of Si3N4 nanowires doping on microstructure and properties of Sn58Bi solder for Cu bonding
Authors: Chen Chen , Zhang Liang , Huang Xi , Lu Xiao 
Published: September 2023

Purpose
The purpose of this study is to delve into the mechanism of Si3N4 nanowires (NWs) in Sn-based solder, thereby furnishing a theoretical foundation for the expeditious design and practical implementation of innovative lead-free solder materials in the electronic packaging industry.

Design/methodology/approach
This study investigates the effect of adding Si3N4 NWs to Sn58Bi solder in various mass fractions (0, 0.1, 0.2, 0.4, 0.6 and 0.8 Wt.%) for modifying the solder and joining the Cu substrate. Meanwhile, the melting characteristics and wettability of solder, as well as the microstructure, interfacial intermetallic compound (IMC) and mechanical properties of joint were evaluated.

Findings
The crystal plane spacing and lattice constant of Sn and Bi phase increase slightly. A minor variation in the Sn58Bi solder melting point was caused, while it does not impact its functionality. An appropriate Si3N4 NWs content (0.2∼0.4 Wt.%) significantly improves its wettability, and modifies the microstructure and interfacial IMC layer. The shear strength increases by up to 10.74% when adding 0.4 Wt.% Si3N4 NWs, and the failure mode observed is brittle fracture mainly. However, excessive Si3N4 will cause aggregation at the junction between the solder matrix and IMC layer, this will be detrimental to the joint.

Originality/value
The Si3N4 NWs were first used for the modification of lead-free solder materials. The relative properties of composite solder and joints were evaluated from different aspects, and the optimal ratio was obtained.
Tourism destination advertising: effect of storytelling and sensory stimuli on arousal and memorability
Authors: Ma Jianyu , Scott Noel , Wu Yu 
Published: September 2023

Purpose
Tourism destination marketers use videos that incorporate storytelling and visual and audio components to evoke emotional arousal and memorability. This study aims to examine the increase in participants’ level of arousal and the degree of memorability after watching two different videos.

Design/methodology/approach
A quasi-experimental study was conducted with 45 participants who watched two destination promotional videos. One video used storytelling whereas the other used scenic images and music. The level of arousal was measured using both tonic and phasic electrodermal activity levels. The memorability of each video was measured after seven days by testing the recall accuracy.

Findings
Scenic imagery and music videos were associated with higher-than-average arousal levels, while storytelling videos generated larger-amplitude arousal peaks and a greater number of arousal-evoking events. After a week, the respondents recalled more events from the storytelling video than from the scenery and musical advertisements. This finding reveals that the treatment, storytelling and sensory stimuli in advertising moderate the impact of arousal peaks and memorability.

Originality/value
These results indicate that nonnarrative videos using only sceneries and music evoked a higher average level of arousal. However, memorability was associated with higher peak levels of arousal only in narrative storytelling. This is the first tourism study to report the effects of large arousal peaks on improved memorability in advertising.
Validation of key sponge symbiont pathways using genome‐centric metatranscriptomics
Authors: O'Brien Paul A. , Tan Shangjin , Frade Pedro R. , Robbins Steven J. , Engelberts J. Pamela , Bell Sara C. , Vanwonterghem Inka , Miller David J. , Webster Nicole S. , Zhang Guojie , Bourne David G. 
Published: September 2023

AbstractThe sponge microbiome underpins host function through provision and recycling of essential nutrients in a nutrient poor environment. Genomic data suggest that carbohydrate degradation, carbon fixation, nitrogen metabolism, sulphur metabolism and supplementation of B‐vitamins are central microbial functions. However, validation beyond the genomic potential of sponge symbiont pathways is rarely explored. To evaluate metagenomic predictions, we sequenced the metagenomes and metatranscriptomes of three common coral reef sponges: Ircinia ramosa, Ircinia microconulosa and Phyllospongia foliascens. Multiple carbohydrate active enzymes were expressed by Poribacteria, Bacteroidota and Cyanobacteria symbionts, suggesting these lineages have a central role in assimilating dissolved organic matter. Expression of entire pathways for carbon fixation and multiple sulphur compound transformations were observed in all sponges. Gene expression for anaerobic nitrogen metabolism (denitrification and nitrate reduction) were more common than aerobic metabolism (nitrification), where only the I. ramosa microbiome expressed the nitrification pathway. Finally, while expression of the biosynthetic pathways for B‐vitamins was common, the expression of additional transporter genes was far more limited. Overall, we highlight consistencies and disparities between metagenomic and metatranscriptomic results when inferring microbial activity, while uncovering new microbial taxa that contribute to the health of their sponge host via nutrient exchange.