bluuebunny/crossref_metadata_2025_split huggingface dataset:
Intravenous thrombolysis and endovascular therapy for acute ischemic stroke in COVID-19: a systematic review and meta-analysis
Authors: Stuckart Isabella , Kabsha Ahmed , Siepmann Timo , Barlinn Kristian , Barlinn Jessica 
Published: August 2023

BackgroundThe impact of COVID-19 on clinical outcomes in acute ischemic stroke patients receiving reperfusion therapy remains unclear. We therefore aimed to synthesize the available evidence to investigate the safety and short-term efficacy of reperfusion therapy in this patient population.MethodsWe searched the electronic databases MEDLINE, Embase and Cochrane Library Reviews for randomized controlled trials and observational studies that investigated the use of intravenous thrombolysis, endovascular therapy, or a combination of both in acute ischemic stroke patients with laboratory-confirmed COVID-19, compared to controls. Our primary safety outcomes included any intracerebral hemorrhage (ICH), symptomatic ICH and all-cause in-hospital mortality. Short-term favorable functional outcomes were assessed at discharge and at 3 months. We calculated pooled risk ratios (RR) and 95% confidence intervals (CI) using DerSimonian and Laird random-effects model. Heterogeneity was evaluated using Cochran’s Q test and I2 statistics.ResultsWe included 11 studies with a total of 477 COVID-19 positive and 8,092 COVID-19 negative ischemic stroke patients who underwent reperfusion therapy. COVID-19 positive patients exhibited a significantly higher risk of experiencing any ICH (RR 1.54, 95% CI 1.16–2.05, p &lt; 0.001), while the nominally increased risk of symptomatic ICH in these patients did not reach statistical significance (RR 2.04, 95% CI 0.97–4.31; p = 0.06). COVID-19 positive stroke patients also had a significantly higher in-hospital mortality compared to COVID-19 negative stroke patients (RR 2.78, 95% CI 2.15–3.59, p &lt; 0.001). Moreover, COVID-19 positive stroke patients were less likely to achieve a favorable functional outcome at discharge (RR 0.66, 95% CI 0.51–0.86, p &lt; 0.001) compared to COVID-19 negative patients, but this difference was not observed at 3-month follow-up (RR 0.64, 95% CI 0.14–2.91, p = 0.56).ConclusionCOVID-19 appears to have an adverse impact on acute ischemic stroke patients who undergo reperfusion therapy, leading to an elevated risk of any ICH, higher mortality and lower likelihood of favorable functional outcome.Systematic review registrationPROSPERO, identifier CRD42022309785.
Brain organoid data synthesis and evaluation
Authors: Brémond-Martin Clara , Simon-Chane Camille , Clouchoux Cédric , Histace Aymeric 
Published: August 2023

IntroductionDatasets containing only few images are common in the biomedical field. This poses a global challenge for the development of robust deep-learning analysis tools, which require a large number of images. Generative Adversarial Networks (GANs) are an increasingly used solution to expand small datasets, specifically in the biomedical domain. However, the validation of synthetic images by metrics is still controversial and psychovisual evaluations are time consuming.MethodsWe augment a small brain organoid bright-field database of 40 images using several GAN optimizations. We compare these synthetic images to the original dataset using similitude metrcis and we perform an psychovisual evaluation of the 240 images generated. Eight biological experts labeled the full dataset (280 images) as syntetic or natural using a custom-built software. We calculate the error rate per loss optimization as well as the hesitation time. We then compare these results to those provided by the similarity metrics. We test the psychovalidated images in a training step of a segmentation task.Results and discussionGenerated images are considered as natural as the original dataset, with no increase of the hesitation time by experts. Experts are particularly misled by perceptual and Wasserstein loss optimization. These optimizations render the most qualitative and similar images according to metrics to the original dataset. We do not observe a strong correlation but links between some metrics and psychovisual decision according to the kind of generation. Particular Blur metric combinations could maybe replace the psychovisual evaluation. Segmentation task which use the most psychovalidated images are the most accurate.
Sinapic acid alleviates inflammatory bowel disease (IBD) through localization of tight junction proteins by direct binding to TAK1 and improves intestinal microbiota
Authors: Jang Sehyeon , Kim San , So Bo Ram , Kim Younghoon , Kim Chang-Kil , Lee Jeong Jae , Jung Sung Keun 
Published: August 2023

Introduction: Although sinapic acid is found in various edible plants and has been shown to have anti-inflammatory properties including colitis, its underlying mechanism and effects on the composition of the gut microbiota are largely unknown. We aimed to identify an early response kinase that regulates the localization of tight junction proteins, act at the onset of the inflammatory response, and is regulated by sinapic acid. Additionally, we analyzed the effects of sinapic acid on the homeostasis of the intestinal microbiome.Methods: We examined the aberrant alterations of early response genes such as nuclear factor-kappa B (NF-κB) and activating transcription factor (ATF)-2 within 2 h of sinapic acid treatment in fully differentiated Caco-2 cells with or without lipopolysaccharide and tumor necrosis factor (TNF)-α stimulation. To confirm the effect of sinapic acid on stimulus-induced delocalization of tight junction proteins, including zonula occludens (ZO)-1, occludin, and claudin-2, all tight junction proteins were investigated by analyzing a fraction of membrane and cytosol proteins extracted from Caco-2 cells and mice intestines. Colitis was induced in C57BL/6 mice using 2% dextran sulfate sodium and sinapic acid (2 or 10 mg/kg/day) was administrated for 15 days. Furthermore, the nutraceutical and pharmaceutical activities of sinapic acid for treating inflammatory bowel disease (IBD) evaluated.Results: We confirmed that sinapic acid significantly suppressed the stimulus-induced delocalization of tight junction proteins from the intestinal cell membrane and abnormal intestinal permeability as well as the expression of inflammatory cytokines such as interleukin (IL)-1β and TNF-α in vitro and in vivo. Sinapic acid was found to bind directly to transforming growth factor beta-activated kinase 1 (TAK1) and inhibit the stimulus-induced activation of NF-κB as well as MAPK/ATF-2 pathways, which in turn regulated the expression of mitogen-activated protein kinase (MLCK). Dietary sinapic acid also alleviated the imbalanced of gut microbiota and symptoms of IBD, evidenced by improvements in the length and morphology of the intestine in mice with colitis.Discussion: These findings indicate that sinapic acid may be an effective nutraceutical and pharmaceutical agent for IBD treatment as it targets TAK1 and inhibits subsequent NF-κB and ATF-2 signaling.
Development and initial validation of the Career Self-Management scale for Chinese coaches
Authors: Zhang Chonghui , Liu Jing-Dong 
Published: August 2023

ObjectiveThe purpose of the study was to develop and initially validate a context-specific scale assessing Career Self-Management for Chinese coaches (Career Self-Management Scale-CC; CSMS-CC).MethodsFirstly, qualitative data obtained from in-depth interview with coaches were contently analyzed to generate potential CSMS-CC items. The content validity of the items was evaluated by a panel of experts. Secondly, the factor structure and item performance of the CSMS-CC were examined using exploratory factor analysis (EFA) and internal consistency reliability of its subscales were evaluated in sample 1 (n= 229, 24.01% females). Thirdly, factor structure of the CSMS-CC was further examined using confirmatory factor analysis (CFA) in sample 2 (n= 295, 32.54% females). Internal consistency reliability was evaluated using Cronbach’ alpha coefficient and composite reliability. Nomological validity was examined using Pearson correlation and structural equation modeling (SEM) by investigating the correlations between CSMS-CC subscales with career success. Finally, measurement invariance and latent mean difference of the CSMS-CC was examined across gender, professional title and coaching class using multiple-group CFA (MGCFA).ResultsBased on the results of the content analysis and content validity evaluation, 18 CSMS-CC items were retained for further analysis. Results of EFA in sample 1 revealed that eight items were problematic and removed. The second round of EFA revealed that three components were retained and labelled as Networking Behavior (4 items), Training Exploration (3 items), and Guanxi Development (3 items). Results of CFA in sample 2 suggested that the 10-item three-correlated-factors model of CSMS-CC demonstrated acceptable model fit to the data, χ2= 135.01, df = 32,p&lt; 0.01, CFI = 0.91, TLI = 0.90, SRMR = 0.05, RMSEA = 0.092 (90% CI = 0.076–0.108). Composite reliability (ranging from 0.84 to 0.88) and Cronbach’s alpha coefficients (ranging from 0.78 to 0.81) of three subscales were found satisfactory. Nomological validity was supported by the results that total score and subscale scores of the CSMS-CC were significantly associated with internal marketability and external marketability. It was found that the CSMS-CC measurement model was strict invariant across gender, professional title and coaching class. Significant differences on all three subscales across professional title and on Guanxi development across coaching class were revealed.ConclusionResults of this study provided initial support for the psychometric properties of the 10-item CSMS-CC, which suggested that the CSMS-CC could be used for measuring the career self-management of Chinese coaches.
Piloting, testing and scaling parental training: a multi-partnership approach in Côte d’Ivoire
Authors: Anago Romuald , Forzy Tiphaine , Guei Sosthene , Pelras Charlotte , Ramde Samuel , Tevenart Camille , Vera Rueda Julieta , Macours Karen 
Published: August 2023

Background and objectivesEarly Childhood Development is high on the policy agenda in Côte d’Ivoire, where the government has identified it as part of its overall approach to improve human capital outcomes. This paper describes a multi-partner approach to piloting, monitoring, adaption, testing and scaling of parental training for ECD. It discusses the learnings from the pilots, and present early evaluation results from two RCTs, focusing on parental participation in trainings and acceptability of messages, with the objective to inform national scaling strategies. As such, this paper illustrates how “MEL systems contributed to ensuring that positive early childhood development (ECD) outcomes were improved as interventions were seeking to achieve scale,” one of the research questions outlined in the call description for the special issue. The paper further provides a real-world example of “How MEL systems can support contributions and buy-in from a variety of stakeholders as ECD interventions (seek to) achieve impacts at scale (e.g., through the public system)?MethodsFive training approaches to improve caregivers’ knowledge and practices around nutrition, preventive health, stimulation, and disciplining were piloted at small scale between 2018 and 2020. An intensive process evaluation was embedded to identify strengths and weaknesses, adapt through an iterative phase, and ultimately make recommendations for their scale up against 11 defined criteria. In early 2021, the two most promising approaches were scaled through two clustered randomized control trials to more than 150 villages each. A cost-effectiveness study was designed in consultation with government stakeholders, centered around targeting different caregivers and decision makers in the household and the extended family and on enhancing community interactions around ECD.ResultsThe evaluation of the five pilots identified one model recommended to be scaled, and one other model to scale after further adaptations. Monitoring and evaluation data from the two models at scale show high levels of participation and acceptability of core messages. Experimental variations involving community champions and fathers increase participation.ConclusionThe iterative and multi-partner process led to two models of parenting training that have wide acceptability. Future work will analyze impacts on cognitive and socio-emotional outcomes, together with cost analysis.
A coalition-driven examination of organization capacity to address food insecurity in Greater Houston: a qualitative research study
Authors: John Jemima C. , Gonzalez Jennifer , Chan Sara-Grace , McPherson Heidi , Aiyer Jennifer N. , Galvan Esperanza , Browning Nicole , Sharma Shreela V. 
Published: August 2023

BackgroundEconomic and social hardships have worsened food insecurity, particularly among low income and racial-ethnic minority groups. Given the core goal of the 150+ member Houston Health Equity Collective (HEC) to reduce food insecurity by 5% in 2025, we explored member organizations' capacity and challenges faced in screening and responding to food insecurity through care coordination efforts.MethodsA twice-administered Qualtrics XM survey (Provo, Utah) with 76 organizations, followed by five focus groups with 22 of these organizations, explored reach and response efforts to food insecurity. Qualitative assessments lasted between 0.5 to 1.5 h, were audio-recorded, cleaned, coded, and thematically analyzed using NVivo, version 11 (Burlington, Massachusetts). The qualitative study was guided by a general inductive approach. In total, over 6 h of audiovisual recording were extracted, and over 100 pages of text exported to NVivo for data analysis. The research team read and coded transcripts independently using the codebook, and met routinely to discuss and resolve codes -resulting in numerous revisions to the codebook. Coding structure was discussed at multiple meetings and differences were addressed through consensus. Predominant qualitative themes impacting food insecurity screening were “stigma and cultural-related barriers”, “clinic capacity and attitudes”, “need to focus on upstream influences of food insecurity and SDOH needs”, “impact of COVID-19”, and “need for HEC system responses”. Main recommendations to enhance screening and reach included improving staff culture, enhancing cultural sensitivity across organizational practices, and using shared technology to coordinate care. Respondents stated that the HEC can drive these recommendations through networking opportunities, use of shared resource directory, and placing focus on upstream factors.ConclusionsRecommendations to target food insecurity must focus on organizational staff responsiveness and sensitivity to patients' needs. Of equal importance is the need for increased attention to the upstream influencers and integration of systems-level interventions to holistically target the barriers impacting food insecurity.
“I fear those things”: non-uptake of contraceptives, and barriers to use among adolescent girls and young women at high risk of HIV infection in Kampala, Uganda
Authors: Kawuma Rachel , Lunkuse Jane Frances , Ssembajjwe Wilber , Kayesu Ivy , Price Matt A. , Brickley Debbie B. , Abaasa Andrew , Mayanja Yunia 
Published: August 2023

BackgroundAdolescent girls and young women involved in risky behaviors are vulnerable to multiple health problems, yet sexual and reproductive health services remain underutilized. We evaluated factors associated with non-uptake of contraceptives and barriers to use among adolescent girls and young women (14–24 years old) at high risk of HIV infection in an environment where contraceptives were provided at no cost.MethodsWe conducted a mixed methods study, utilizing data from a baseline cross sectional survey and qualitative in-depth interviews. Survey participants tested negative for pregnancy and reported willingness to use contraception. Non-uptake of contraceptives was defined as not taking contraception at any study visit (baseline and throughout the study). Logistic regression model was used to assess factors associated with non-uptake of contraceptives. We purposively selected participants for interviews to discuss their knowledge and experiences with contraceptives and make suggestions to improve uptake. Qualitative data were analyzed thematically.ResultsAll 285 participants were included in the analysis. Out of the 285 participants 127 were not using contraceptives and of the 127, 44 (34.6%) did not take up any method throughout the study while 43 of the 83 remaining participants (who took up a method) chose male condoms only. Non-uptake of contraceptives was less likely among older women (20–24 years) (aOR = 0.32, 95% CI 0.16–0.89) compared to younger women (less than 20 years). Qualitative data showed that concerns about future fertility, fear of associated side effects and influence from close relations contributed to non-uptake of contraception.ConclusionNon-uptake of contraceptives was common despite the promotion and provision of contraceptives in the context of a research study mainly because adolescents lack autonomy while making contraceptive decisions. Identifying and addressing their concerns and continued counselling on contraceptive use alongside condom promotion may improve uptake and utilization of contraceptives.
Genome-Wide Identification and Analysis of Collar Region-Preferential Genes in Rice
Authors: Jiang Xu , Hong Woo-Jong , Lee Su-Kyoung , Jung Ki-Hong 
Published: August 2023

The collar region plays a crucial role in leaf angle formation and plant architecture, which is important for improving crop yield given the challenges of diminishing arable land and changing environmental conditions. To determine collar region-preferential genes (CRPGs) affecting plant architecture and crop yield, we conducted genome-wide transcriptomic analysis. By integrating our RNA sequencing data with public rice anatomical expression data, we identified 657 CRPGs. Verification involved testing six randomly selected CRPGs, all of which exhibited collar-preferential expression. The functional significance of CRPGs was assessed via Gene Ontology enrichment analysis, utilizing MapMan and KEGG, and literature analysis provided additional information for characterized CRPGs. Our findings revealed links between manipulating leaf angle and phytohormone-related pathways and stress responses. Moreover, based on the CRPGs, five transcription factors downstream of the liguleless 1 (LG1) gene were identified. Overall, the identified CRPGs provide potential targets for further research and breeding applications aimed at improving crop productivity by manipulating leaf architecture.
Age and Growth of Hedinichthys yarkandensis (Day, 1877) in the Hotan River
Authors: Wang Xin-Yue , Chen Sheng-Ao , Song Yong , Wang Cheng-Xin , Liu Fei 
Published: August 2023

Hedinichthys yarkandensis (Day, 1877) has been highlighted in research and evaluated as a class II key protected aquatic wildlife in Xinjiang Uygur Autonomous Region. To enhance the study of fish resources in the Hotan River, further germplasm conservation of fishery resources specific to the Tarim River Basin should be carried out, and the development of the aquatic seed industry should be promoted. A total of 1275 H. yarkandensis individuals were collected from 2020–2021. Fish ecological methods were used to explore the population ecological characteristics and ecological habits of H. yarkandensis. We found that the age of H. yarkandensis ranged from one to seven based on lapillus otoliths, and two-plus individuals accounted for more. The age structure composition reveals stable genetic performance and good population fecundity. The fitting correlations of standard length and body weight reveal that H. yarkandensis in the Hotan River is a uniformly growing fish. The ratio of females to males is 0.87:1. The Fulton condition index of males was slightly higher than that of females due to individual miniaturization. The comparative study found that the growth parameters of the Hotan River population (L∞ = 302.772, W∞ = 310.8450, t0 = −0.4608) were higher than those of the other groups. The feeding demand of H. yarkandensis in the Hotan River was guaranteed because the effect of human activities was small in the watershed area and the watershed ecosystem was more stable, which resulted in stable germplasm resources in the Hotan River population.
The Use of Integral Citation in English Students’ Final Project Reports
Authors: Nugraha Danny , Khorina Mulyati , Agoes Farida , Sulyaningsih Iis 
Published: August 2023

This paper is concerned with integral citation employed by English students of Politeknik Negeri Bandung in their Final Project Reports (FPR). Thus, the aims of this study were to investigate the most integral citation type used by the students in their FPR. In conducting the study, 18 FPR focusing on analysis produced in 2022 were collected as data source. Applying qualitative method and text analysis, the results reveal several things. First, the students favor integral citation than non-integral citation. Second, verb controlling appears more than naming. In verb controlling citation, lexical verb state is commonly used compared with other lexical verbs. Whereas in naming, the phrase according to + the cited authors is the mostly used. this study has implication in improving the students’ academic skills, especially in doing citation.
ULTRASOUND-ASSISTED EXTRACTION SENYAWA ANTIOKSIDAN DARI LIMBAH BIJI PEMBUATAN MINYAK BUAH MERAH (Pandanus conoideus)
Authors: Wehantouw Frenly , Situngkir Risma , Suoth Elly 
Published: August 2023

The aim of this research was to obtain antioxidant active component from Papuan red fruit oil waste using Ultrasound-assisted extraction. The research was done started from the making of Papuan red fruit oil, extraction and bioactive component analysis (total tocopherol and betacaroten) using spectrophotometry. The result shows that waste from Papuan red fruit oil process contains high amount of yield at 20 minutes extraction time with total carotenoid 20, 23 ppm and tocopherol absorbance 1.04. Ultrasound assisted extraction method significantly increase carotenoid content aw well as tocopherol (p<0.05).
Kadar Flavonoid Total Ekstrak Etanol Daun Jarak Pagar (Jatropha curcas L) Dengan Metode Spektrofotometri UV-Visibe
Authors: Nova Ramadhan Krisdiyanto  , Muhammad Saad  
Published: August 2023

Terdapat beberapa aktifitas biologis dalam daun jarak pagar antara lain sebagai
antidiabetes dan antihipertensi. Flavonoid adalah senyawa metabolit sekunder yang
bertanggung jawab terhadap aktifitas tersebut. Penelitian ini bertujuan untuk
menghitung kandungan total flavonoid ekstrak daun jarak pagar, serta
mengidentifikasi kandungan alkaloid, terpenoid, saponin, flavonoid, dan tanin yang
terdapat pada ekstrak daun jarak pagar. Uji skrining fitokimia pada penelitian ini
dilakukan dengan penambahan reagen dan dilakukan pengamatan terhadap perubahan
warna yang terjadi. Penetapan kadar dilakukan dengan metode spektrofotometri UVVis menggunakan reagen AlCl3, dengan baku pembanding yaitu quersetin. Identifikasi
senyawa ekstrak daun jarak pagar pada penelitian ini mendapatkan hasil positif
saponin, terpenoid, flavonoid, tanin, dan alkaloid. Konsentrasi flavonoid total ekstrak
didapatkan sebesar 1,649706 mgQE/g, dengan %KV 0,021909%. Daun jarak pagar
dapat menjadi alternatif sumber flavanoid, sebagai bahan obat alami.