bluuebunny/crossref_metadata_2025_split huggingface dataset:
The Implications of Recent Developments in Online Artificial Intelligence Apps for Interdisciplinary Studies in the Humanities
Authors: Patke Rajeev S 
Published: January 2025

The recent developments in Artificial Intelligence (AI) have produced varied types of global impact over the last year. In that context, one might ask the following questions: (a) why has AI become (bad) news so suddenly? (b) what is the prehistory of present concerns? By way of answering these questions, this essay will, first, discuss the various negative implications of AI and the spread of various applications that use AI for a variety of tasks and functions. It will then narrow its focus to address the more specific negative implications of AI as they are likely to affect Asia as a region with its own specific socio-economic and political matrix for its educational institutions. The focus narrows further to address the likely impact of AI on the Humanities. The third and final part of the essay stands back from the focus on education in Asia to look at two mitigating features of the case: (a) the various checks and balances that are currently being proposed globally regarding how the negative aspects of AI might be controlled, and (b) a consideration of the various ways in which developments in AI could benefit mankind, and more specifically, Humanities education in Asia. Moreover, the essay will also consider the ways in which AI applications are likely to play a central role in how we study, teach, learn, and work in the future. To take stock of what is just round the corner might prepare us better, as individuals, groups and institutions to work with and around rather than against the growth of AI.
Spectrum, Volume 18, June 2023: 1-9
Implementation of Nggahi Rawi Pahu Philosophy Through in Chemistry Learning Based on Social Cognitive Theory for Student Character Strengthening
Authors: Annafi Nurfidianty , Admyana Putu Budi , Suastra I Wayan , Sutajaya I Made , Tika I Nyoman , Wesnawa I Gede Astra 
Published: January 2025

Social cognitive theory developed by Albert Bandura is the most influential approach to learning. This theory emphasizes that a person's behavior can be learned through observation of social models in the surrounding environment, be it teachers, parents, or peers. This theory is known as observational learning or modeling. This research aims to explore the process of observational learning in an effort to strengthen the value of the Nggahi Rawi Pahu philosophy. The research method used is descriptive qualitative. The sample in this study were fourth semester students of the Chemistry Education Study Program. Data collection techniques were carried out by means of observation, administration of questionnaires and documentation. In this study, observational learning was carried out through 4 stages of cognition in the learning process, namely attention, retention, motor reproduction and motivation. 7 out of 10 students showed a positive response to the relevance, involvement and benefits of observational learning in an effort to strengthen the philosophy of Nggahi Rawi Pahu. This shows that through observational learning the value of the Nggahi Rawi Pahu philosophy can be improved.
Oscillatory fluid flow enhanced mineralization of human dental pulp cells
Authors: Yortchan Witsanu , Puwanun Sasima 
Published: January 2025

The purpose of this study is to evaluate the optimum frequency of oscillatory fluid flow (OFF) for increasing osteogenesis in human dental pulp cells (DPCs) in an incubating rocking shaker. DPCs from 3 donors were cultured in an osteogenic induction medium (OIM) and mechanical stimulation was applied using an incubating rocking shaker at frequencies of 0 (control), 10, 20, 30, and 40 round per minute (RPM) for 1 h/day, 5 days/week. Cell proliferation was measured using total protein quantification, and osteogenic activity was measured by alkaline phosphatase (ALP) activity, calcium deposition, and collagen production on days 7, 14, and 21 of culture. Results of DPCs morphology in the 30 RPM group were more clustered and formed interconnections between cells. Results of DPC proliferation and collagen production showed no significant differences between the experiment groups. The ALP activity on day 7 and 14, and calcium deposition on day 21 of the 30 RPM group were significantly higher than the control groups. Thus 30 RPM is likely an effective frequency for increasing calcium deposition. This study uses strategies in Tissue Engineering followed the research topic about an application of human cells to stimulate oral and maxillofacial hard tissue regeneration. In the future, the mineralization of DPCs could be enhanced by using an incubating rocking shaker at 30 RPM in the lab to create a cell sheet. The mineralized cell sheet could then be implanted into the patient for bone repair of orofacial defects.
Predictive value of NT-pro BNP on outcomes of children with ventricular septal defect surgery
Authors: Chen Weidan , Tang Yajie , Lu Ye , Ma Li , Chen Xinxin , Liu Techang 
Published: January 2025

BackgroundLimited study has shown whether NT-proBNP is related to the prognosis of children wth ventricular septal defect (VSD) surgery. The study was conducted to determine the predictive value of NT-proBNP on outcomes of children with VSD surgery.MethodsA total of 798 children with VSD surgery were enrolled, with NT-proBNP measured at preoperatively and 24-h postoperatively. The short- and mid-term clinical outcomes were recorded. Propensity scores (PS) was performed to acquire pre-op and post NT-proBNP 24-h PS-matched cohorts for comparisons between groups.ResultsIn the pre NT-proBNP PS-matched cohort, the higher NT pro-BNP group had longer hospitalization time and lower post-op 1-month EF value compared with low NT pro-BNP group (all P &lt; 0.05), and there wasn't significant difference of mechanical ventilation time, cardiopulmonary bypass (CPB) time, intensive care unit (CCU) stay, and ejection fraction (EF) values of 3 month to 12 months after surgery (all P &gt; 0.05). In the post NT-proBNP PS-matched cohort, there wasn't significant difference of mechanical ventilation time, CPB time, CCU stay, hospitalization time, and EF values of 1 month to 12 months after surgery between two groups (all P &gt; 0.05).ConclusionsVSD children with higher pre NT-proBNP level had longer hospital stays after surgery than those with lower level. Pre NT-proBNP level had no effect on mechanical ventilation time, CPB time, ACC time and CCU stay and cardiac function after 3 months postoperatively. Post-op 24-h NT pro-BNP level wasn't associated with clinical outcomes.
Left atrial appendage occlusion and radiofrequency ablation in a patient with atrial fibrillation and dextrocardia: a case report
Authors: Zhang Zengfu , Fu Xiaohong , Guo Min , Gao Jia , Wang Rui 
Published: January 2025

BackgroundDextrocardia is a rare congenital condition, affecting approximately 1 in 10,000–12,000 individuals. When combined with atrial fibrillation (AF), it becomes even rarer. “One-stop” surgery, including combined radiofrequency ablation (RFA) and left atrial appendage occlusion (LAAO), has become a common clinical treatment for patients with AF who develop cerebral infarction despite regular oral anticoagulants. To date, no cases have been reported of patients with AF and dextrocardia undergoing the “one-stop” procedure, making this surgery particularly challenging.Case presentationAn 85-year-old dextrocardia male with total visceral inversion and persistent AF developed cerebral infarction despite regular oral anticoagulation therapy. He was referred to our hospital for RFA of AF and LAAO. The procedure was successfully performed using a three-dimensional electroanatomical mapping system (Carto3, Biosense Webster, Diamond Bar, CA, USA), intracardiac echocardiography (ICE), and x-ray, with no complications.ConclusionThis is the first reported case of a “one-stop” surgery for dextrocardia with AF. This procedure is safe and feasible with the assistance of advanced technologies such as ICE and the VIZIGO bidirectional adjustable bent sheath.
On the obligations of states to respond to climate change and China’s legal consequences: based on the advisory opinion in case no. 31 of the international tribunal for the law of the sea
Authors: Zeng Wanping , Wang Guihua 
Published: January 2025

IntroductionClimate change presents a critical global challenge, requiring states to develop and implement robust legal and policy frameworks. The 2024 Advisory Opinion issued by the International Tribunal for the Law of the Sea (ITLOS) in Case No. 31 highlights the legal obligations of states under the United Nations Convention on the Law of the Sea (UNCLOS) to address climate change, emphasizing the role of the marine environment in this context.MethodsThis study conducts a comprehensive analysis of the Advisory Opinion and examines its implications for China’s existing climate change legal framework. The analysis identifies key challenges, including expanded legal responsibilities, the harmonization of international law with domestic legislation, and the increased risk of international climate litigation for domestic enterprises.ResultsThe Advisory Opinion establishes a higher standard for China’s legislative response to climate change, necessitating the integration of its international obligations into its domestic legal framework. Key findings reveal gaps in China’s current legal system and emphasize the need for proactive measures to align with international standards.DiscussionTo address these challenges, the paper proposes several recommendations: developing a comprehensive legal framework for climate change, reinforcing due diligence obligations, establishing a robust climate monitoring and assessment system, and enhancing the capacity of domestic enterprises to handle international climate litigation. These measures aim to position China as a proactive contributor to global climate governance.ConclusionThe study provides valuable insights for advancing China’s climate change law and aligning it with the international legal framework. The proposed measures serve as essential guidance for China to assume greater responsibility and influence within the global climate legal system.
Endophytic strategies decoded by genome and transcriptome analysis of Fusarium nematophilum strain NQ8GII4
Authors: Yan Siyuan , Zhang Qingchen , Jia Shuxin , Guo Miaomiao , Zhang Qiangqiang , Gu Peiwen 
Published: January 2025

IntroductionFusarium nematophilum strain NQ8GII4 is an endophytic fungus with significant potential for improving growth and disease resistance of alfalfa. However, the molecular mechanisms underlying the symbiotic relationship between NQ8GII4 and alfalfa roots remain poorly understood.MethodsIn this study, we conducted (1) a comparative genomic analysis of selected saprophytic, pathogenic, and endophytic fungi, including molecular phylogeny analysis, whole-genome alignment, and divergence date estimation positioning, and (2) transcriptomic profiling of alfalfa roots infected with NQ8GII4.ResultsOur findings reveal that NQ8GII4 is genetically closely related to F. solani, suggesting it diverged from Fusarium phytopathogens. During the early stages of symbiosis establishment, genes encoding glycosyltransferases (GTs), fungal cell wall-degrading enzymes (FCWDEs), and steroid-14α-demethylase (CYP51) were significantly downregulated, potentially suppressing hyphal growth of the fungus. Once symbiosis was established, NQ8GII4 secreted effectors that activated plant immunity, which in turn could slow growth of the fungus. Moreover, genes involved in secondary metabolite biosynthesis, such as type I polyketide synthases (T1PKS) and non-ribosomal peptide synthetases (NRPSs), were significantly downregulated. Homologs of autophagy-related genes, including ATG1, ATG2, ATG11, and others, were also downregulated, suggesting that reduced phytotoxin production and autophagy inhibition is a consequence of NQ8GII4’s symbiosis.DiscussionThis study investigated the comprehensive molecular and genetic mechanisms governing the interaction between NQ8GII4 and alfalfa roots. Beyond the NQ8GII4-alfalfa system, these findings also provide a valuable molecular framework for understanding the mechanism of interactions between endophytic fungi and their host plants.
Innovative antifungal strategies: enhanced biofilm inhibition of Candida albicans by a modified tea tree oil formulation
Authors: Tuan Dang Anh , Uyen Pham Vu Nhat , Khuon Nguyen Van , Binh Ly An , Masak Jan 
Published: January 2025

IntroductionCandida albicans is a significant human pathogen with the ability to form biofilms, a critical factor in its resistance to antifungal treatments. This study aims to evaluate the antifungal activity and biofilm inhibition potential of Tea Tree Oil (TTO) derived from Melaleuca alternifolia cultivated in Vietnam.MethodsThe antifungal activity of TTO was assessed by determining the Minimum Inhibitory Concentration (MIC), Minimum Fungicidal Concentration (MFC), Minimum Biofilm Inhibitory Concentration (MBIC), and Minimum Biofilm Eradication Concentration (MBEC) using broth dilution methods. The experiments were conducted on C. albicans in both planktonic and biofilm states across concentrations ranging from 0.1 μL/mL to 10 μL/mL.ResultsTTO demonstrated significant antifungal efficacy, with a MIC of 0.1 μL/mL (∼91.217 μg/mL) and an MFC of 10 μL/mL (∼9121.7 μg/mL). It effectively inhibited biofilm formation with a recorded MBIC of 2 μL/mL (∼1824.34 μg/mL). However, MBEC values were not determinable as the concentrations tested did not achieve the eradication of more than 50% of mature biofilm within the experimental conditions.DiscussionThese findings highlight TTO as a promising natural antifungal agent with strong biofilm-inhibitory properties. However, its limited efficacy in eradicating mature biofilms underscores the need for further studies, potentially involving higher concentrations or synergistic combinations with conventional antifungal agents.
Development and validation of an explainable machine learning prediction model of hemorrhagic transformation after intravenous thrombolysis in stroke
Authors: Lin Yanan , Li Yan , Luo Yayin , Han Jie 
Published: January 2025

ObjectiveTo develop and validate an explainable machine learning (ML) model predicting the risk of hemorrhagic transformation (HT) after intravenous thrombolysis.MethodsWe retrospectively enrolled patients who received intravenous tissue plasminogen activator (IV-tPA) thrombolysis within 4.5 h after symptom onset to form the original modeling cohort. HT was defined as any hemorrhage on head CT scan completed within 48 h after IV-tPA administration. We utilized the Random Forest (RF), Multilayer Perceptron (MLP), Adaptive Boosting (AdaBoost), and Gaussian Naive Bayes (GauNB) algorithms to develop ML-HT models. The models' predictive performance was evaluated using confusion matrix (including accuracy, precision, recall, and F1 score), and discriminative analysis (area under the receiver-operating-characteristic curve, ROC-AUC) in the original cohort, followed by validation in an independent external cohort. The models' explainability was assessed using SHapley Additive exPlanations (SHAP) global feature plot, SHAP Summary Plot, and Partial Dependence Plot.ResultsA total of 1,007 patients were included in the original modeling cohort, with an HT incidence of 8.94%. The RF-based ML-HT model showed metrics of 0.874 (accuracy), 0.972 (precision), 0.890 (recall), 0.929 (F1 score); with ROC-AUC of 0.7847 in the original cohort and 0.7119 in the external validation cohort. The MLP model showed 0.878, 0.967, 0.989, 0.978, 0.7710, and 0.6768, respectively. The AdaBoost model showed 0.907, 0.967, 0.989, 0.978, 0.7798, and 0.6606, respectively. The GauNB model showed 0.848, 0.983, 0.598, 0.716, 0.6953, and 0.6289, respectively. The explainable analysis of the RF-based ML model indicated that the National Institute of Health Stroke Scale (NIHSS) score, age, platelet count, and atrial fibrillation were the primary determinants for HT following IV-tPA thrombolysis.ConclusionThe RF-based explainable ML model demonstrated promising predictive ability for estimating the risk of HT after IV-tPA thrombolysis and may have the potential to assist the clinical decision-making in emergency settings.
Tracking changes in autonomic function by coupled analysis of wavelet-based dispersion of heart rate variability and gastrointestinal symptom severity in individuals with hypermobile Ehlers–Danlos syndrome
Authors: Mathena Sarah A. , Allen Robert M. , Laukaitis Christina , Andrews Jennifer G. 
Published: January 2025

IntroductionPeople with hypermobile Ehlers–Danlos syndrome (hEDS) experience multisystemic dysfunction with varying severity and unpredictability of flare occurrence. Cohort studies suggest that individuals with hEDS have a higher risk for autonomic dysfunction. The gold standard for assessing autonomic function, clinically, is the heart rate variability (HRV) assessment from 24-h Holter monitor electrocardiogram data, but this is expensive and can only be performed in short durations. Since their advent, biometric devices have been a non-invasive method for tracking HRV to assess autonomic function. This study aimed to understand the intra- and inter-individual variability in autonomic function and to associate this variability with gastrointestinal symptoms in individuals with hEDS using wearable devices.MethodsWe studied 122 days of biometric device data from 26 individuals, including 35 days highlighted as high gastrointestinal (GI) dysfunction and 48 days as low GI dysfunction. Utilizing wavelet analysis to assess the frequency domains of heart rate signals, we compared participants’ HRV data for high, low, very low (VLF), and ultralow (ULF) frequency domains associated with physiological differences.ResultsWe found a significant difference between the VLF and ULF signals on high-GI symptom days compared with low-symptoms days for 92 and 76% of the signals sampled, respectively.DiscussionOur pilot data show a change in HRV for individuals with hEDS experiencing a flare day for a single-body system. Future research will focus on evaluating the relationship between longitudinal multisystemic symptom severity fluctuations and HRV.
Unveiling hotspots of emerging research in the miRNA-related mechanism underlying cancer through comprehensive bibliometric analysis with implications for precision medicine and non-invasive diagnostics
Authors: Zhang Zhirui , Song Wenhuan , Chen Wenyu , Cui Wenze , Chen Wenyi , Zhang Qinheng , Ji Wenwen , Wang Yinglin , Wang Jiayi , Yu Wenhao , Yu Mingkun , Hao Tao , Jiang Hong 
Published: January 2025

Background and objectiveMicroRNAs (miRNAs) are implicated in cancer by exerting roles in tumor growth, metastasis, and even drug resistance. The general trends of miRNA research in diverse cancers are not fully understood. In this work, miRNA-related research in colorectal cancer, prostate cancer, leukemia, and brain tumors was analyzed in search of key research trends with clinical potential.MethodsA bibliometric analysis of articles, spanning from 2014 to 2024, was carried out with the major focus laid on four types of cancers. The Co-citation network analysis, keyword bursts, and the collaborative pattern were done in VOSviewer and CiteSpace, respectively.ResultsColorectal cancer had the highest publication volume, with research primarily focusing on gene expression, extracellular vesicles, and non-coding RNAs. Prostate cancer showed a shift toward clinical applications, while leukemia and brain tumor research, though less extensive, highlighted miRNA’s potential in early diagnosis and treatment. Co-citation analysis identified emerging research collaborations and key contributors.ConclusionmiRNA plays a pivotal role in cancer diagnosis, biomarker development, and therapeutic interventions. With advancements in non-invasive diagnostics and personalized medicine, miRNA offers significant potential for clinical applications. Future research should focus on miRNA’s role in drug resistance and combination therapies to accelerate its clinical translation.
Pharmacokinetics of 7,8-dihydroxyflavone in neonatal mice with hypoxia-ischemia related brain injury
Authors: Lim Sin Yin , Scarlett Cameron O. , Yapici Sefer , Ferrazzano Peter , Cengiz Pelin 
Published: January 2025

Introduction7,8-Dihydroxyflavone (7,8-DHF) is a promising translational therapy in several brain injury models, including the neonatal hypoxia-ischemia (HI) model in mice. However, the neuroprotective effect of 7,8-DHF was only observed in female, but not male, neonatal mice with HI brain injury. It is unknown whether HI-induced physiological changes affect brain distribution of 7,8-DHF differently for male versus female mice. We aimed to evaluate the impact of sex on the pharmacokinetics of 7,8-DHF in plasma and brain neonatal mice following experimentally induced HI brain injury.MethodsLeft-sided HI brain injury was induced in postnatal day 9 (P9) mice, followed by a 5 mg/kg intraperitoneal injection of 7,8-DHF. A liquid chromatography-tandem mass spectrometry method was developed to quantitate the drug concentration in plasma samples, as well as in samples from the left and right brain hemispheres. A nonlinear mixed-effects model was used to analyze the plasma and brain concentration-time data. A semi-quantitative approach was used to evaluate the concentrations of two active O-methylated metabolites of 7,8-DHF (8H7M-flavone and 7H8M-flavone) in both plasma and brain samples.ResultsOur PK analyses show that plasma 7,8-DHF concentrations followed a two-compartment PK model, with more than 95% eliminated by 3 h after the IP injection. Sex was not significantly associated with the PK of 7,8-DHF; however, HI brain injury was associated with a 21% reduction in clearance (p &lt; 0.01). The distribution of 7,8-DHF to the brain was rapid; however, the extent of brain distribution was low with the right and left brain-to-plasma partition coefficients being 8.6% and 9.9%, respectively. Additionally, both O-methylated metabolites of 7,8-DHF were detected in the plasma and brain.ConclusionThe plasma and brain PK of 7,8-DHF in neonatal mice were similar between males and females. The low extent of 7,8-DHF brain distribution and the potential effects of the active metabolites should be considered in future studies evaluating the therapeutic effects of 7,8-DHF.