bluuebunny/crossref_metadata_2025_split huggingface dataset:
Functional traits of soil nematodes define their response to nitrogen fertilization
Authors: Hou Weichen , Kuzyakov Yakov , Qi Yanwen , Liu Xiang , Zhang Hui , Zhou Shurong 
Published: February 2023

Abstract

Nitrogen (N) fertilization and warming are two crucial global change factors affecting the soil nematode communities. The effects of N fertilization and warming, however, on nematode communities in soils are inconsistent across ecosystems and maybe be even opposite.

One key reason is that the commonly used taxonomic diversity is less sensitive to environmental changes than the seldom‐used trait‐based indicators. To verify this, we performed an 8‐year field experiment with four N fertilization levels with and without soil warming and collected an extensive dataset consisting of (i) six traits related to the nematode performance, that is, body size, maximum body length, maximum body width, stylet length, oesophagus length and intestinal length; (ii) the taxonomic alpha (richness and abundance) and beta diversity (Bray–Curtis dissimilarities) of the whole nematode community and each nematode functional group, (iii) soil food web resources (the total taxonomic richness and abundance of plant, bacterial and fungal communities) and (iv) soil properties (pH, total, ammonium and nitrate N, microbial N and C, total and available P and soil water content).

We found that N fertilization altered plant diversity and soil nitrate levels, which in turn decreased taxonomic alpha diversity of two nematode functional groups (phytophagous nematodes and predators), but taxonomic diversity for the whole nematode community remained stable. The decreased taxonomic alpha diversity of phytophagous nematodes resulted in increased maximum body width, but decreased stylet length and oesophageal length. Mild warming (~ 0.7°C) had no effects on soil properties and soil food web resources, and the taxonomic diversity or nematode traits remained unchanged.

Our results reveal that nematode functional traits show strong responses to N fertilization as individual nematode groups adapted quickly to changed soil properties and food web resources. Taxonomic diversity indices, however, were more stable under these changes showing that the functional composition of nematode communities may respond in the short term despite little effects on species diversity. Thus, the trait‐based indicators not only reveal how nematodes respond to N fertilization, but also how nematodes mediate the effects of N fertilization on ecosystem functioning (i.e. soil nutrient cycling).

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High species turnover and unique plant–pollinator interactions make a hyperdiverse mountain
Authors: Minachilis Konstantinos , Kantsa Aphrodite , Devalez Jelle , Vujic Ante , Pauly Alain , Petanidou Theodora 
Published: February 2023

Abstract

We studied α‐ and β‐diversity of pollinators, flowering plants and plant–pollinator interactions along the altitudinal gradient of Mt. Olympus, a legendary mountain and biodiversity hotspot in Central Greece.

We explored 10 study sites located on the north‐eastern slope of the mountain, from 327 to 2596 m a.s.l. Insect surveys were conducted once a month using hand netting (years 2013, 2014 and 2016), and they were combined with recordings of flowering plant diversity (species richness and flower cover). We then calculated α‐ and β‐diversity of pollinators, plants in flower and plant–pollinator interactions, and explored their demographic response along the altitudinal gradient.

Alpha diversity of pollinators, plants and plant–pollinator interactions were altitude dependent; α‐diversity of all pollinators, bees, non‐bumblebee bees, bee flies and butterflies showed linear declines with altitude, whereas those of hoverflies and bumblebees showed unimodal patterns. Beta diversity and its turnover component of all pollinators, hoverflies, bees, bumblebees, non‐bumblebee bees, butterflies and plants showed linear increases, whereas those of bee flies and of plant–pollinator interactions varied independently from the pairwise altitudinal difference.

The high dissimilarity and uniqueness of pollination networks, which is probably a result of the high biodiversity and endemism of Mt. Olympus, is driven by species turnover and the formation of new interactions between new species. Contrasting to the monotonic decline of the remaining groups, the unimodal patterns of hoverfly and bumblebee α‐diversity are probably the effect of a higher tolerance of these groups to high‐altitude environmental conditions. Our findings highlight that the high turnover of species and of pollination interactions along the altitudinal gradient are the mainstay of hyperdiverse mountains, a fact that conveys important historical, ecological and conservational implications.
Make me a test and I will save the world: towards an anthropology of the possible in global health
Authors: Street Alice 
Published: February 2023

AbstractWhat is deemed possible in the wake of failure? The global biotech industry's failure to develop affordable diagnostic devices for use in low‐ and middle‐income countries (LMICs) has inspired a generation of humanitarian entrepreneurs to launch their own diagnostic start‐up companies. This essay traces the rise and fall of one such start‐up in Boston in the United States, Daktari, which developed a portable HIV testing device in the 2010s. I show how the alignment of humanitarian and economic valuations in a single diagnostic device depended on the ability of the start‐up's founders and employees to synchronize the distinct tempos of financial capital, humanitarian design, and global health standards. Yet their failure to achieve this synchronization does not simply offer another story of biotech hype and speculation, the generation of promissory value at the expense of actual things. Instead, the essay examines the hopes and expectations that the firm's employees invested in the device's material qualities, manufacture, and distribution. What, I ask, is the meaning of failure, and its material remains, when it is measured against humanitarian rather than solely commercial expectations? The essay concludes with some reflections on the aftermath of failure in humanitarian entrepreneurship, examining the questions that Daktari's demise poses for understandings of what is possible and desirable in global health.
Can computed tomography replace or supplement autopsy?
Authors: Lathrop Sarah L. , Wiest Philip W. , Andrews Sam W. , Elifritz Jamie , Price Janet P. , Mlady Gary W. , Zumwalt Ross E. , Gerrard Chandra Y. , Poland Valerie L. , Nolte Kurt B. 
Published: February 2023

AbstractPostmortem computed tomography (PMCT) has been integrated into the practice of many forensic pathologists. To evaluate the utility of PMCT in supplementing and/or supplanting medicolegal autopsy, we conducted a prospective double‐blind comparison of abnormal findings reported by the autopsy pathologist with those reported by a radiologist reviewing the PMCT. We reviewed 890 cases: 167 with blunt force injury (BFI), 63 with pediatric trauma (under 5 years), 203 firearm injuries, and 457 drug poisoning deaths. Autopsy and radiology reports were coded using the Abbreviated Injury Scale and abnormal findings and cause of death (COD) were compared for congruence in consensus conferences with novel pathologists and radiologists. Overall sensitivity for recognizing abnormal findings was 71% for PMCT and 74.6% for autopsy. Sensitivities for PMCT/autopsy were 74%/73.1% for BFI, 61.5%/71.4% for pediatric trauma, 84.9%/83.7% for firearm injuries, and 56.5%/66.4% for drug poisoning deaths. COD assigned by reviewing PMCT/autopsy was correct in 88%/95.8% of BFI cases, 99%/99.5% of firearm fatalities, 82.5%/98.5% of pediatric trauma deaths, and 84%/100% of drug poisoning deaths of individuals younger than 50. Both autopsy and PMCT were imperfect in recognizing injuries. However, both methods identified the most important findings and are sufficient to establish COD in cases of BFI, pediatric trauma, firearm injuries and drug poisoning in individuals younger than 50. Ideally, all forensic pathologists would have access to a CT scanner and a consulting radiologist. This would allow a flexible approach that meets the diagnostic needs of each case and best serves decedents' families and other stakeholders.
Role of DSC1 in Drosophila melanogaster synaptic activities in response to haedoxan A
Authors: Qie Xingtao , Ren Yaxin , Chen Xueting , Du Yuzhe , Dong Ke , Hu Zhaonong 
Published: February 2023

AbstractDrosophila sodium channel 1 (DSC1) encodes a voltage‐gated divalent cation channel that mediates neuronal excitability in insects. Previous research revealed that DSC1 knockout Drosophila melanogaster conferred different susceptibility to insecticides, which indicated the vital regulation role of DSC1 under insecticide stress. Haedoxan A (HA) is a lignan compound isolated from Phryma leptostachya, and we found that HA has excellent insecticidal activity and is worthy of further study as a botanical insecticide. Herein, we performed bioassay and electrophysiological experiments to test the biological and neural changes in the larval Drosophila with/without DSC1 knockout in response to HA. Bioassay results showed that knockout of DSC1 reduced the sensitivity to HA in both w1118 (a common wild‐type strain in the laboratory) and parats1 (a pyrethroid‐resistant strain) larvae. Except for parats1/DSC1−/−, electrophysiology results implicated that HA delayed the decay rate and increased the frequency of miniature excitatory junctional potentials of Drosophila from w1118, parats1, and DSC1−/− strains. Moreover, the neuromuscular synapse excitatory activities of parats1/DSC1−/− larvae were more sensitive to HA than DSC1−/− larvae, which further confirmed the functional contribution of DSC1 to neuronal excitability. Collectively, these results indicated that the DSC1 channel not only regulated the insecticidal activity of HA, but also maintained the stability of neural circuits through functional interaction with voltage‐gated sodium channels. Therefore, our study provides useful information for elucidating the regulatory mechanism of DSC1 in the neural system of insects involving the action of HA derived from P. leptostachya.
Domestication effects on nitrogen allocation, internal recycling and nitrogen use efficiency in the perennial new crop Silphium integrifolium (Asteraceae)
Authors: Gonzalez‐Paleo Luciana , Ravetta Damián A. , Vilela Alejandra E. , Van Tassel David 
Published: February 2023

AbstractImprovements in seed yield during domestication and breeding are frequently achieved moving plants from the conservative syndrome of the plant economic spectrum towards the more acquisitive side, changing how plants acquire, allocate, use and store C and nutrients in relation to their wild relatives. The aims were to evaluate if domestication changed the N allocation, the internal plant N recycling (N resorption efficiency and proficiency, and N storage) and N‐use‐efficiency, in the perennial new crop Silphium integrifolium. We compared in a field experiment repeated in two locations (Kansas and Patagonia), a Wild (W) and two improved accessions, with high‐ (HYI) and low‐seed yield improved (LYI) accessions. HYI accessions produced more than twice the biomass and acquired twofold more N than LYI and Wild accessions. Changes in proportional N allocation were similar in both improved accessions (HYI and LYI) and locations: higher allocation to leaves and lower to the crown at pre‐anthesis (growth‐storage trade‐off) and higher allocation to seeds and lower to the crown at maturity (reproduction‐storage trade‐off). HYI and LYI allocated 50% more N to seeds in average, reducing a 31% the N allocated to the crown in relation to the Wild. Nitrogen use efficiency (NUE) and mean residence time (MRT) of N were reduced in both improved accessions. In HYI, the lower MRT was related to the lower storage of N in the crown and N removal through seed production, and in LYI was the result of the N lost through leaf senescence. HYI produced litter with lower N concentration (more proficient) that the wild accession. These could decrease the net N mineralization rate and soil N availability at long term. HYI plants should require higher external N inputs to the soil to sustain their N uptake requirements. LYI and Wild accessions, had lower resorption efficiency, but N recycled was allocated to the crown for future remobilization and uses (better internal cycling). The leaf litter type of these accessions with higher N content should sustain faster N‐cycles rates and N release to the soil. If the focus is on the ecosystem services, HYI accessions could help achieve a reduction in nitrate leaching by maximizing N uptake, N resorption and N removal through seed (i.e. nitrate catch crop), and also produce crop residues that slow down biogeochemical cycling and soil nutrient retention.
The acute effects of cannabis with and without cannabidiol in adults and adolescents: A randomised, double‐blind, placebo‐controlled, crossover experiment
Authors: Lawn Will , Trinci Katie , Mokrysz Claire , Borissova Anna , Ofori Shelan , Petrilli Kat , Bloomfield Michael , Haniff Zarah R. , Hall Daniel , Fernandez‐Vinson Natalia , Wang Simiao , Englund Amir , Chesney Edward , Wall Matthew B. , Freeman Tom P. , Curran H. Valerie 
Published: February 2023

AbstractBackground and AimsLong‐term harms of cannabis may be exacerbated in adolescence, but little is known about the acute effects of cannabis in adolescents. We aimed to (i) compare the acute effects of cannabis in adolescent and adult cannabis users and (ii) determine if cannabidiol (CBD) acutely modulates the effects of delta‐9‐tetrahydocannabinol (THC).DesignRandomised, double‐blind, placebo‐controlled, crossover experiment. The experiment was registered on ClinicalTrials.gov (NCT04851392).SettingLaboratory in London, United Kingdom.ParticipantsTwenty‐four adolescents (12 women, 16‐ to 17‐year‐olds) and 24 adults (12 women, 26‐ to 29‐year‐olds) who used cannabis 0.5–3 days/week and were matched on cannabis use frequency (mean = 1.5 days/week).InterventionWe administered three weight‐adjusted vaporised cannabis flower preparations: ‘THC’ (8 mg THC for 75 kg person); ‘THC + CBD’ (8 mg THC and 24 mg CBD for 75 kg person); and ‘PLA’ (matched placebo).MeasurementsPrimary outcomes were (i) subjective ‘feel drug effect’; (ii) verbal episodic memory (delayed prose recall); and (iii) psychotomimetic effect (Psychotomimetic States Inventory).FindingsCompared with ‘PLA’, ‘THC’ and ‘THC + CBD’ significantly (P < 0.001) increased ‘feel drug effect’ (mean difference [MD] = 6.3, 95% CI = 5.3–7.2; MD = 6.8, 95% CI = 6.0–7.7), impaired verbal episodic memory (MD = –2.7, 95% CI = −4.1 to −1.4; MD = −2.9, 95% CI = −4.1 to −1.7) and increased psychotomimetic effects (MD = 7.8, 95% CI = 2.8–12.7; MD = 10.8, 95% CI = 6.2–15.4). There was no evidence that adolescents differed from adults in their responses to cannabis (interaction P ≥ 0.4). Bayesian analyses supported equivalent effects of cannabis in adolescents and adults (Bayes factor [BF01] >3). There was no evidence that CBD significantly modulated the acute effects of THC.ConclusionsAdolescent cannabis users are neither more resilient nor more vulnerable than adult cannabis users to the acute psychotomimetic, verbal memory‐impairing or subjective effects of cannabis. Furthermore, in adolescents and adults, vaporised cannabidiol does not mitigate the acute harms caused by delta‐9‐tetrahydocannabinol.
RNAseq reveals modulation of genes involved in fatty acid biosynthesis in chicken liver according to genetic background, sex, and diet
Authors: Perini Francesco , Wu Zhou , Cartoni Mancinelli Alice , Soglia Dominga , Schiavone Achille , Mattioli Simona , Mugnai Cecilia , Castellini Cesare , Smith Jacqueline , Lasagna Emiliano 
Published: February 2023

AbstractIncreases in chicken production are mainly due to specialised breeds. However, local breeds are of increasing importance, known for ability to adapt to the environment and unique products. Conventional poultry products contain lower levels of n‐3 fatty acids (FAs) compared to those obtained from local breeds, therefore the aim of this study was to evaluate the modulation of expression of genes involved in long‐chain polyunsaturated FA (PUFA) biosynthesis pathways according to genetic background, diet conditions, and sex. Animals from two local breeds and a commercial line were fed different diets: control and experimental diet (10% linseed supplementation). For each breed and diet group, both sexes were reared. The RNA was extracted from 36 liver samples and sequenced by RNAseq method. Bioinformatic analysis was carried out to find differentially expressed genes from comparisons between experimental groups. Results showed low impact of diet on differentially expressed genes related to FA biosynthesis, but linseed diet increased percentage of n‐3 FAs of liver. Sex and genetic background determined the differential expression of genes related to long‐chain PUFA biosynthesis. Specifically, females of local breeds shared 23 up‐regulated genes when compared to their respective commercial line groups. Some of the shared genes had a role in de novo triglyceride biosynthesis (MTTPL and GPAM), and in de novo FA biosynthesis (ACACA and SCD) was detected. In conclusion, local breeds are able to better adapt to a diet rich in PUFA, by triggering certain transcriptomic shifts in the liver that allow birds to process the high PUFA content provided by diet.
Differences in intent to refer buprenorphine among community correctional and treatment staff: A set of cross‐lagged models predicting efficacy beliefs and familiarity with buprenorphine for opioid use disorder
Authors: Milano Nicole , Lister Jamey J. , Victor Grant 
Published: February 2023

AbstractBackground and ObjectivesDespite high rates of individuals with opioid use disorder, community correctional agencies underutilize medications for opioid use disorder (MOUD). Knowledge about the mechanisms which motivate correctional employees to refer buprenorphine remains underdeveloped, and differences in these patterns by employee status are unknown. This study has two objectives: (1) investigate the presence of a reciprocal relationship between familiarity with buprenorphine and efficacy beliefs among community corrections and community treatment staff and (2) identify whether this relationship differs by staff status in referral intentions.MethodsData were used from the Criminal Justice Drug Abuse Treatment Studies 2 (CJ‐DATS 2) among correctional and treatment employees (N = 873). Four models investigated whether a reciprocal relationship existed between buprenorphine familiarity and efficacy beliefs. Then, the best fitting model was used to test the influence that prior training had on future referral intention through familiarity and efficacy beliefs among the analytic sample (n = 612), by comparing two separate structural equation models (SEMs) among correctional staff and treatment staff, respectively.ResultsThe fully cross‐lagged model provided a significantly better fit to the data than other models ( (1) = 7.189, p < .01). The results of the multigroup SEM show that training had positive, indirect effects on future referral intentions that significantly differed between treatment and community correction staff.Discussion and ConclusionsFindings show that training may influence correctional staff intent to refer individuals to receive buprenorphine through familiarity.Scientific SignificanceTailored training for MOUD treatment for specific staff populations may prove more beneficial than existing approaches.
Effectiveness of local regulations on nonpoint source pollution: Evidence from Wisconsin dairy farms
Authors: Skidmore Marin , Andarge Tihitina , Foltz Jeremy 
Published: February 2023

AbstractAlthough the Clean Water Act's regulation of point source pollution has had a significant effect on water quality, nonpoint sources of pollution, especially animal agriculture, remain a leading unevenly regulated source of water pollution in the United States. This work studies the effectiveness of local manure management regulations on dairy farms in Wisconsin. Wisconsin represents a unique policy experiment in the delegation of nonpoint agricultural pollution policy in a state with economically important small, nonpoint, dairy farms. Using hand collected regulatory data from Wisconsin counties we estimate the effects of changes in local regulations on water quality outcomes. The results demonstrate that a few easily implemented and verifiable regulations such as nutrient management plans have significant short‐term effects on water quality, whereas other less observable and difficult to implement regulations have no discernible effects in the short term. The work points to a number of potential policy levers to improve the Management of nonpoint pollution, as well as the challenges of nonpoint source regulatory policies on slow‐moving hydrologic processes.
Retinal microvascular markers in type 2 diabetes subphenotypes and latent autoimmune diabetes of adults
Authors: Pedersen Frederik N. , Stidsen Jacob V. , Rasmussen Martin N. , Nielsen Henning‐Beck , Henriksen Jan Erik , Olesen Thomas Bastholm , Olsen Michael Hecht , Nielsen Jens S. , Højlund Kurt , Blindbæk Søren Leer , Grauslund Jakob 
Published: February 2023

AbstractPurposeTo estimate if newly diagnosed patients with different subphenotypes of type 2 diabetes (T2DM) or latent autoimmune diabetes of adults (LADA) differ with respect to subclinical retinal microvascular structure or diabetic retinopathy (DR).MethodsThis population‐based, cross‐sectional study of 340 patients (675 eyes) classified patients with recently diagnosed T2DM in different subphenotypes according to beta cell function and insulin sensitivity in to; classical (n = 218), hyperinsulinaemic (n = 86), insulinopenic (n = 20), or LADA (n = 16). Retinal 6‐field images were graded according to the International Clinical DR Severity Scale by a retinal expert. Retinal microvascular structures were analysed in eyes by a semiautomatic software.ResultsMedian age and duration of diabetes were 58.1 (49.9; 65.5) and 0.9 (0.5; 2.4) years, respectively, and 56.8% were male. In a multivariate linear mixed model regression analysis of eyes without DR (n = 570), there was no statistically significant difference in retinal venular or arteriolar width between subtypes and patients with classical T2DM. In addition, eyes from different subphenotypes did not differ according to vessel density, tortuosity or fractal dimension. In a multivariate logistic regression model adjusted for age, sex, HbA1c, diabetes duration, body mass index, mean arterial blood pressure and history of cardiovascular disease, there was a tendency towards persons with hyperinsulinaemic T2DM to be more likely to have DR (OR 1.97, 95% CI 0.95; 4.09) compared to classical T2DM.ConclusionWe found no difference in retinal microvascular structure in patients with newly diagnosed subtypes of T2DM. However, DR may be more prevalent in newly diagnosed patients with hyperinsulinaemic T2DM compared to individuals with classical T2DM.