bluuebunny/crossref_metadata_2025_split huggingface dataset:
Combining Biosafety Experts’ Evaluations and Workers’ Perceptions to Disclose Biological Risks in Biomedical Laboratories
Authors: Vourtsis Dionysios , Papageorgiou Efstathia , Kriebardis Anastasios , Karikas George Albert , Van Willigen Gijsbert , Kotrokois Kostas , Dounias Georgios , Karkalousos Petros 
Published: January 2025

Objectives: This paper focuses on examining the biological risks in biomedical laboratories of public hospitals in Athens, Greece, by combining expert evaluations and laboratory workers’ perception. It also aimed to review how personnel managed biological materials, assess the level of safety awareness and training of workers, and propose hazards mitigation actions according to local legislation and international biosafety guidelines. Materials and Methods: A total of 36 biosafety level 2 (BSL-2) laboratories in 20 hospitals were evaluated for biosafety containment specifications and adherence to biosafety procedures. The study was conducted as a cross-sectional survey using a checklist and a detailed health and safety (H&S) questionnaire, focusing on biosafety and biorisk management. An expert biosafety officer inspected and completed a checklist for each laboratory (n=36) across the 20 hospitals. Additionally, 415 lab professionals completed a biosafety-specific health and safety questionnaire. Results: Both the checklists and questionnaires revealed that, although some positive findings were observed, a significant percentage of laboratories lacked effective management of biological agents and materials overall. The main deficiencies identified included restricted access, safety equipment, standard operating procedures (SOPs), biorisk management systems, risk assessments, biosafety manuals, biosafety officers, accident reporting, and biosafety training programs. Conclusion: The significant shortcomings in some areas of engineering and administrative controls, as well as in the implementation of Greek and European biosafety legislation, demonstrate that the laboratories do not fully comply with internationally accepted BSL-2 standards. Therefore, there is an urgent need for more comprehensive and proactive measures, adequate biosafety training for workers, and stricter enforcement of existing laws and directives to ensure the safety of laboratory professionals, the community, and the environment.
PD-1 Blockade Enhances the Anti-Tumor Immune Response Induced by Cryoablation in A Murine Model of Lung Cancer
Authors: FENG Jianyu , LIU Hui , MU Feng , NIU Lizhi , ZENG Jianying 
Published: January 2025

Abstract

Purpose
To investigate the efficacy of cryoblation plus PD-1 inhibitor in the treatment of mouse lung cancer, and its possible mechanism on regulating cellular immune function.
Materials and Methods
Murine lung cancer xenograft model was constructed. Mice were randomly divided into Control, Cryo + IgG, Anti-PD-1, and Cryo + Anti-PD-1 group, and given corresponding treatment. Tumor growth (n = 10) and survival of (n = 10) were observed. Flow cytometry was used to detect the proportion of CD4 + T cells, CD8 + T cells, and regulatory T (Treg) cells.
Results
Tumor volume of the three treatment groups were significantly smaller than that of the Control group (P<0.01), those of Anti-PD-1 or Cryo + Anti-PD-1group were much smaller than that of the Cryo + IgG group (P<0.05). The medium survival time of the three treatment groups were significantly longer than that of the Control group (P<0.01). The proportions of CD4 + T cells in the peripheral blood, spleen and tumor of the three treatment groups were significantly lower than that of the Control group (P<0.05), while the CD8 + T cells were markedly higher than that of the Control group (P<0.05). The proportions of Treg cells in the spleen of the three treatment groups were notably lower than that of the Control group (P<0.05). mRNA level of IFN-γ was increased while IL-10 was decreased in the three treatment groups (P<0.01).
Conclusion
Either cryoblation or PD-1 inhibitor treatment alone enhances the anti-cancer effect of the body immune system by down-regulating the proportion of CD4 + T cells and Treg cells, while up-regulating the proportion of CD8 + T cells. Notably, the combination of cryoblation and PD-1 inhibitor get better effect on lung cancer treatment.
Frailty Prevalence And Evaluation Of The FRAIL Scale Questionnaire In Patients with Inflammatory Bowel Disease
Authors: Bahirwani Janak , Elmer Joshua , Eskarous Hany , Ahuja Suruchi Jai Kumar , Changela Madhav , Dahiya Dushyant Singh , Stoltzfus Jill , Schneider Yecheskel 
Published: January 2025

Abstract

Background
Inflammatory bowel disease (IBD) predisposes individuals to frailty, linked with adverse outcomes. While the Fried Frailty Index (FFI) is a well-established phenotypic tool to assess frailty, its administration is cumbersome. The FRAIL scale, simpler but not widely used in IBD patients, presents an alternative. We aimed to assess the prevalence of frailty and compare the FRAIL scale with the FFI.
Methods
Two hundred and nine confirmed IBD patients underwent assessment using both the FFI and the FRAIL scale. Patients were categorized as non-frail, pre-frail, or frail. Pearson coefficients evaluated correlation, and unadjusted analyses assessed frailty risk factors. ROC curve analysis evaluated the FRAIL scale.
Results
Among participants (53.5% female, median age 44), 37% were non-frail, 50% pre-frail, and 13% frail. The FRAIL scale exhibited strong correlation with the FFI for all three categories. Age showed no significant association with frailty. Frail individuals displayed higher inflammatory markers and more severe clinical disease, with frailty more prevalent in UC than CD patients. Frail individuals also exhibited lower hemoglobin, creatinine, and albumin levels.
Conclusion
Frailty and pre-frailty are prevalent in the IBD population, not necessarily linked with older age. The FRAIL scale demonstrated excellent correlation with the FFI, offering a practical tool for identifying frailty in IBD without physical measurements. Future studies should explore multivariable models incorporating frailty risk factors and interventions to mitigate adverse outcomes in the IBD population.
Evaluating the Benefits of Tegafur-Uracil Adjuvant Therapy in Low-Risk Stage IIa Colon Cancer: Insights from a Decade-Long Study
Authors: Lin Chang-Lin , Chiang Feng-Fan , Lin Bo-Zh , Chen Ming-Cheng , Lin Chun-Yu , Huang Shang-Chih 
Published: January 2025

Abstract

Background
Surgical resection is the gold standard for treating non-metastatic colorectal cancer. The five-year overall survival (OS) for Stage IIIa patients is longer than that for Stage IIa patients, highlighting the importance of adjuvant therapy (ACT) for Stage IIa. This study aims to evaluate the treatment outcomes and prognostic factors for Stage IIa colon cancer at our hospital over the past decade.
Materials and Methods
This retrospective study was conducted at a medical center and included patients treated between January 1, 2010, and December 31, 2019. We included only patients who underwent surgery and had pathological Stage IIa or IIIa colon cancer. Collected data included patient characteristics, oncological outcomes, receipt of adjuvant therapy, and adverse effects associated with adjuvant therapy.
Result
We observed a higher five-year OS for Stage IIIa than Stage IIa patients, but the difference was not statistically significant. Patients who were administered tegafur-uracil had longer five-year progression-free survival and five-year overall survival compared to the No ACT group (p < 0.005). The receiver operating characteristic curve identified the optimal cut-off point for tegafur-uracil (UFT) duration as ≤ 1.74 years, with the area under the curve being statistically significant (p = 0.05).
Conclusion
ACT is recommended even for low-risk Stage IIa patients postoperatively. The adverse effects of UFT were generally acceptable, with Grade 3 or higher adverse effects being rare. If UFT is used as an ACT regimen for stage IIa colon cancer, a duration of more than one year is suggested.
The Significance of Anatomic Sites in Diffuse Large B-Cell Lymphoma Recurrence
Authors: wang siqian , xu xi , ma yongyong , qian shanhu , tang liyuan , sun lan , shen zhijian , ye haige , qian honglan , jiang songfu , Zhou Shujuan 
Published: January 2025

Abstract

Objective:This study aimed to investigate the anatomic patterns and outcomes of first relapse in patients with diffuse large B-cell lymphoma (DLBCL).
Materials and Methods: Between January 2015 and October 2024, a total of 1,047 newly diagnosed patients were evaluated, of which 139 patients who initially achieved complete remission (CR) but subsequently experienced relapse during follow-up were included in the analysis. Histological and imaging data were reviewed to determine the involvement of nodal regions, extranodal locations, and Waldeyer’s Ring at the time of relapse.
Results: Our findings indicated that 112 patients (80.4%) presented with clinical manifestations that led to the diagnosis of recurrence. Two-thirds of patients relapsed within 18 months, and 85% within two years, with a median time to relapse of 9 months. Notably, 43.2% of patients experienced a change in the anatomic site of recurrence, which correlated with poorer clinical outcomes. The overall CR rate after relapse was 36.7% for those relapsing at previously involved sites, compared to just 10.0% for those with new site relapses. Higher CR rates were also observed in patients with relapses at previously involved sites from Waldeyer’s Ring (50% CR; 8 of 16) and extranodal lymphomas (40% CR; 8 of 20), compared to those relapsing at new sites (P=0.019 and P=0.006, respectively). Overall survival(OS) was significantly lower in patients with relapses at new sites (23.0 ± 4.2 months) compared to those at previously involved sites (41.8 ± 6.1 months). Kaplan-Meier analysis revealed significant differences in OS and progression-free survival(PFS) based on the site of relapse (P=0.043 and P=0.004, respectively). Patients experiencing relapses at new sites, particularly involving Waldeyer’s Ring and extranodal origins, had significantly poorer outcomes, with two-year OS rates of 16.7% and 8.3%, respectively (P=0.032 and P=0.006).
Conclusions: Relapsed DLBCL tends to primarily affect previously involved sites, especially in patients with nodal origins. Recurrences at new sites are associated with poor outcomes, particularly when the initial anatomic sites were Waldeyer’s Ring and extranodal.
Extracellular NAD + Modulates CD203a Expression on Th17 Cells and Predicts Long-Term Recurrence-Free Survival in Hepatocellular Carcinoma
Authors: Babigian Julia , Brunnbauer Philipp , Kamali Can , Knitter Sebastian , Keshi Eriselda , Felsenstein Matthäus , Haber Philipp , Lozzi Isis , Schöning Wenzel , Pratschke Johann , Krenzien Felix 
Published: January 2025

Abstract

Background and Aims: Mortality rates for hepatocellular carcinoma (HCC) remain high, while multimodal treatment approaches offer new perspectives. Here, we investigated the influence of extracellular nicotinamide adenine dinucleotide (eNAD +) on ecto-nucleotide pyrophosphatase/phosphodiesterase 1 (CD203a, ENPP1 or PC-1) on Th17 cells in relation to the likelihood of HCC recurrence following liver resection.
Method: The study compared heparinized blood plasma samples from 95 patients who underwent liver resection, including 20 patients with HCC and 10 control patients without liver disease. Plasma eNAD + concentrations were determined using a heat-based dichotomous pH extraction method, followed by enzymatic cycling and a colorimetric assay for quantification. Fibrosis was graded histologically using the Desmet score (F0-F4). Surface expression analysis was performed using flow cytometry.
Results: With increasing grades of liver fibrosis grade a significant reduction in plasma eNAD + concentrations was measured (p < 0.05). Further, a significant correlation was found between eNAD + levels and CD203a expression on CD4 + , CCR4 + as well as CCR6 + T cells (p < 0.05). Patients who exhibited high proportions of CD203a expressing Th17 cells (CD4 + , CCR6 + CCR4 +) post surgery were found to be at a 6-fold increased risk (HR 6.38, 95% Cl 1,51-27.00) of HCC recurrence and had a median recurrence-free survival of 233 days (p < 0.05), compared to patients with low CD203a expressing Th17 cells (CD4 + CCR6 + CCR4 +). Similarly, patients who had a high proportion of CD203a expressing Th17 cells (CD4 + CCR6 +) following surgery had a 5-fold increased risk (HR 5.56, 95% Cl 1.58-19.59) of HCC recurrence and a median recurrence-free survival of 334 days (p < 0.05) compared to those with low CD203a expressing Th17 cells (CCR6 +).
Conclusion: The data indicate that eNAD + levels are decreased in patients with liver fibrosis or cirrhosis, and are correlated with the expression of ectoenzyme CD203a on Th17 cells. Consequently, patients with high expression of CD203a on Th17 cells had a significantly increased likelihood of recurrence, indicating their potential as valuable prognostic markers and a possible therapeutic target.
Inequities and Disparities: An Investigation of Antenatal Visits in Mozambique
Authors: Gopan Adwaith , Kumar B.J Binu , Johnson Neena S , VS Pooja Sasi 
Published: January 2025

Background
Mozambique is a southeastern sub-Saharan African country with the highest maternal, infant, and neonatal mortality rates. Low antenatal visits were often associated with high levels of these indicators. This study examined the changes in antenatal care and the factors affecting antenatal visits for policy making.

Methods
Data from the Mozambique DHS (2022-23) were used for the analyses. The variables were selected from the individual-interpersonal-community framework, and zero-inflated Poisson regression was used to test the factors associated with antenatal visits.

Results
Higher education among women (IRR:1.057; OR: 0.34) and partner’s education (IRR:1.155), having a job [either agriculture (OR:0.341) or non-agriculture (IRR: 1.077; OR: 0.341)], higher wealth (IRR>1; OR<1), and use of the Internet (IRR:1.046) were positively associated with antenatal visits. Problems in obtaining permission (OR:2.35), not wanting to go alone (OR: 1.97), longer distances (IRR: 0.966), and being in a rural area (OR:2.38) were negatively associated with antenatal visits.

Conclusion
Antenatal visits to Mozambique were severely limited by individual, interpersonal, and community inequities. These can be overcome by tailored policymaking addressing these barriers instead of a single and uniform maternal care policy.
Pengaruh Self Efficacy Terhadap Intention to Participate Dengan Social Influence Sebagai Variabel Moderasi
Authors: Maghfira Nisa , Handrito Radityo Putro 
Published: January 2025

The purpose of this quantitative explanatory study is to identify the effects of self-efficacy on the intention to participate in cosplay events in Malang City with social influence as the moderating variable. Using non-probability sampling method and purposive sampling technique, 156 people with the minimum age of seventeen years who live in Malang City and know about cosplay events in Malang City were selected as the respondents, regardless their participation in the events. This research uses validity test, reliability test, and classical assumption test that were performed in the Statistical Package for the Social Sciences (SPSS). The results of the multiple linear regression and moderated regression analysis indicate that self-efficacy (X) and social influence (Z) partially have positive and significant effects on the intention to participate in the cosplay events in Malang City. Further, social influence (Z) is not significant in enhancing the positive effect of self-efficacy on the intention to participate in cosplay events in Malang City.

Abstrak
Penelitian ini bertujuan untuk mengetahui pengaruh Self Efficacy terhadap Intention to Participate dengan Social Influence sebagai variabel moderasi pada Event Cosplay di Kota Malang. Jenis penelitian ini adalah explanatory research dengan metode kuantitatif. Penelitian ini menggunakan sampel sebanyak 156 responden, Pengambilan sampel menggunakan teknik non probability sampling dengan metode purposive sampling. Kriteria responden yang dibutuhkan dalam penelitian ini adalah berusia minimal 17 tahun, berdomisili di Kota Malang, mengetahui tentang apa itu Event Cosplay di Kota Malang beserta kegiatannya, dan yang sudah pernah maupun belum pernah mengikuti Event Cosplay. Penelitian ini menggunakan Statistical Package for the Social Sciences (SPSS) untuk melakukan uji validitas, uji reliabilitas dan uji asumsi klasik. Teknik analisis data menggunakan uji regresi linier sederhana dan moderated regression analysis. Berdasarkan hasil pengujian hipotesis diperoleh data variabel Self Efficacy (X) dan Social Influence (Z) berpengaruh positif dan signifikan secara parsial terhadap Intention to Participate pada Event Cosplay di Kota Malang. Namun, Social Influence (Z) tidak signifikan dalam memperkuat pengaruh positif Self Efficacy terhadap Intention to Participate pada Event Cosplay di Kota Malang.
Pengaruh Ewom Terhadap Purchase Intention
Authors: Bangun Muhammad Fathurrahman , Ismail Taufiq 
Published: January 2025

This study aims to determine how Electric Word of Mouth influences Purchase intention for Kahf products on the TikTok application among Generation Z in Malang City. This type of research is explanatory research, which explains or proves the relationship between independent and dependent variables. This study uses a sample of 242 respondents, with questionnaires distributed online through Google Forms. The sampling method used is non-probability sampling with a purposive sampling technique. The sample consists of respondents who are Generation Z in Malang City. Data analysis employs PLS-SEM analysis using SmartPLS 4.0. The results show that the Information adoption variable positively and significantly influences the Purchase intention variable, the Information usefulness variable positively and significantly influences the Information adoption variable, the Information quality variable positively and significantly influences the Information usefulness variable, the Information credibility variable positively and significantly influences the Information usefulness variable, and the Information quantity variable also positively and significantly influences the Information usefulness variable.

Abstrak
Penelitian ini bertujuan untuk mengetahui bagaimana pengaruh Electric Word of Mouth terhadap Purchase intention produk Kahf di aplikasi TikTok pada Generasi Z di Kota Malang. Jenis penelitian ini adalah explanatory research yang menjelaskan atau membuktikan hubungan pengaruh antar variabel independen dan dependen. Penelitian ini menggunakan sampel sebanyak 242 responden yang mana penyebaran kuesioner dilakukan melalui Google formulir secara online. Pengambilan sampel menggunakan metode non-probability sampling dengan teknik purposive sampling. Sampel terdiri dari responden yang merupakan Generasi Z yang ada di Kota Malang. Analisis data menggunakan Analisis PLS-SEM dengan menggunakan SmartPLS 4.0. Hasil penelitian menunjukan bahwa variabel Information adoption secara positif dan signifikan memengaruhi variabel Purchase intention, variabel Information usefulness secara positif dan signifikan memengaruhi variabel Information adoption, variabel Information quality secara positif dan signifikan memengaruhi variabel Information usefulness, variabel Information credibility secara positif dan signifikan memengaruhi variabel Information usefulness dan variabel Information quantity juga secara positif dan signifikan memengaruhi variabel Information usefulness.
Item analysis of pre-service EFL teachers’ formative test in teaching practicum for program evaluation
Authors: Hartati Neti 
Published: January 2025

Teaching practicum is crucial for pre-service EFL teachers' professional development. Many studies have investigated student-teachers' performance in teaching practicum in various aspects. However, research on their performance in constructing test items in teaching practicum has not been found. Test construction is an essential pedagogical competence that student-teachers must master. Investigating their performance in test construction may give valuable feedback to student-teachers and for teacher education. To fill the research gap, this study conducted an item analysis of a formative test constructed by two student-teachers in a teaching practicum. It revealed that the DP of the items have good quality but the FV analysis showed there is no difficult item suggesting that they cannot make difficult questions. The test reliability is high (0.900625) but there are 6 invalid items. Three items contain grammatical errors creating students' confusion influencing the FV, DP, and the validity. It revealed that the test makers' grammar mastery may determine item quality. It suggests student-teachers improve their grammar mastery, and teacher education improves the quality of English grammar teaching and refine their curriculum of evaluation and language testing subjects and include the evaluation of student-teachers' performance in language assessment in teaching practicum program as a focus.
Transformative Gamified Binocular Therapy for Unilateral Amblyopia in Young Children: Pilot Prospective Efficacy and Safety Study
Authors: Zhu Wenqing , Gu Shuneng , Li Jian , Lin Jin , Hu Chanling , Liu Rui 
Published: January 2025

Abstract

Background
Amblyopia is a common cause of visual impairment in children. Compliance with traditional treatments for amblyopia is challenging due to negative psychosocial impacts. Recent shifts in amblyopia treatment have moved from suppressing the dominant eye to enhancing binocular visual function. Binocular digital therapy has become a promising approach.

Objective
The aim of this study was to evaluate the effects of binocular gamified digital therapy on visual acuity and stereoacuity (SA) in children with unilateral amblyopia.

Methods
This pilot prospective study enrolled 11 children aged 4-6 years with unilateral amblyopia. Following at least 8 weeks of refractive correction, participants underwent binocular gamified digital therapy for 60 minutes per day, 5 days a week. The therapy used a roguelike shooting game delivered under binocular conditions through two independent channels with a real-time artificial intelligence visual engine. Assessments of distance visual acuity (DVA), near visual acuity (NVA), and SA were conducted at baseline and again at 4, 8, and 12 weeks.

Results
At 12 weeks, the following significant improvements were noted: amblyopic eye DVA improved by 1.0 line (P=.01; d=0.77), binocular DVA improved by 0.7 lines (P=.006; d=1.00), and SA improved by 0.3 logarithm (log) arcseconds (P=.01; d=0.97). At 8 weeks, improvements included amblyopic eye DVA by 0.9 lines (P=.046; d=1.00) and SA by 0.28 log arcseconds (P=.02; d=0.90). No significant adverse events were observed, although one participant developed progressive esotropia.

Conclusions
Binocular gamified digital therapy is effective and safe for improving visual outcomes in children aged 4-6 years with unilateral amblyopia.