bluuebunny/crossref_metadata_2025_split huggingface dataset:
Adult‐onset leukoencephalopathy with axonal spheroids and pigmented glia caused by a novel mutation of CSF1R gene
Authors: Ding Cong , Zhao Li , Zhong Rujia , Zhan Yu , Dong Chunbo 
Published: June 2023

AbstractBackgroundAdult‐onset leukoencephalopathy with axonal spheroids and pigmented glia (ALSP) is a rare autosomal dominant disorder caused by mutations in the colony‐stimulating factor 1 receptor (CSF1R) gene. As of 2022, more than 100 different CSF1R mutations were reported in patients with CSF1R‐related leukoencephalopathy.MethodIn this case report, we described ALSP in a previously healthy 46‐year‐old woman. The patient underwent detailed neurological examinations including cognitive assessment, brain magnetic resonance imaging (MRI) and whole‐exome sequencing.ResultThe patient manifested as memory impairment, poor interpersonal behavior and decreased verbal fluency. Brain MRI showed confluent white matter changes and atrophy of the corpus callosum. Whole‐exome sequencing identified a novel splice site mutation (C.1858 + 5G > A) in intron 13 of the CSF1R gene, which has not been reported worldwide.ConclusionALSP has a wide range of clinical manifestations and genetic heterogeneity. It should be considered when diagnosing rapidly progressive dementia with or without motor impairment. We recommend biopsy or genetic testing in these patients to avoid misdiagnosis and delayed diagnosis. We will continue to monitor this family in the future to improve our understanding of ALSP, so as to correctly diagnose ALSP in clinic and provide clinical data for better research on the pathogenesis and treatment of this disease.
METTL14‐Mediated m6A Modification of TNFAIP3 Involved in Inflammation in Patients With Active Rheumatoid Arthritis
Authors: Tang Jifeng , Yu Ziqing , Xia Jinfang , Jiang Renquan , Chen Shuhui , Ye Detai , Sheng Huiming , Lin Jinpiao 
Published: June 2023

ObjectiveThe aim of the study was to investigate the role of N6‐methyladenosine (m6A) modification in the progression of rheumatoid arthritis (RA).MethodsPeripheral blood mononuclear cells (PBMCs) from patients with RA and healthy controls were collected. The expression of m6A modification‐related proteins and m6A levels were detected using polymerase chain reaction (PCR), western blot, and m6A enzyme‐linked immunosorbent assay (ELISA). The roles of methyltransferase‐like 14 (METTL14) in the regulation of inflammation in RA was explored using methylated RNA immunoprecipitation (MeRIP) sequencing and RNA immunoprecipitation assays. Collagen antibody‐induced arthritis (CAIA) mice were used as an in vivo model to study the role of METTL14 in the inflammation progression of RA.ResultsWe found that m6A writer METTL14 and m6A levels were decreased in PBMCs of patients with active RA and correlated negatively with the disease activity score using 28 joint counts (DAS28). Knockdown of METTL14 downregulated m6A and promoted the secretion of inflammatory cytokines interleukin 6 (IL‐6) and IL‐17 in PBMCs of patients with RA. Consistently, METTL14 knockdown promoted joint inflammation accompanied by upregulation of IL‐6 and IL‐17 in CAIA mice. MeRIP sequencing and functional studies confirmed that tumor necrosis factor α induced protein 3 (TNFAIP3), a key suppressor of the nuclear factor‐κB inflammatory pathway, was involved in m6A‐regulated PBMCs. Mechanistic investigations revealed that m6A affected TNFAIP3 expression by regulation of messenger RNA stability and translocation in TNFAIP3 protein coding sequence.ConclusionsOur study highlights the critical roles of m6A on regulation of inflammation in RA progression. Treatment strategies targeting m6A modification may represent a new option for management of RA.
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Characterizing the Impact of Oligomerization on Redox Flow Cell Performance
Authors: Weiss Trent A. , Fan Gang , Neyhouse Bertrand J. , Moore Evan B. , Furst Ariel , Brushett Fikile R. 
Published: June 2023

AbstractRedox flow batteries (RFBs) are hindered by complex failure modes, particularly crossover through the membrane, resulting in capacity fade and reduced cycling efficiencies. Redox‐active oligomers (RAOs) have recently been proposed for mitigating this phenomenon while maintaining sufficient transport properties; however, to date, few studies have quantified how the chemical and electrochemical properties of RAOs influence their performance in redox flow cells. Here, we demonstrate that oligomeric derivatives of 2,2,6,6‐tetramethylpiperidine 1‐oxyl (TEMPO) exhibit lower diffusivities than the monomeric species but retain facile charge transfer characteristics. The size‐dependent variations in mass transport rates directly translate to differences in flow cell polarization and symmetric cycling performance. Post‐mortem analyses reveal that oligomerization does not meaningfully alter decay processes as evinced by similar capacity fade across all species. Broadly, these findings corroborate and extend upon previously developed relationships between molecular size, electrochemical properties, and flow cell performance.
Two Layers of Computational Screening on Silaborane‐based Clusters Filter Ca(SiB11H11CH3)2 as the Promising Electrolyte for Calcium‐Ion Batteries
Authors: Sharma Abhiruchi , Thomas Anson , Gupta Puneet 
Published: June 2023

AbstractCalcium‐ion batteries (CIBs) are promising energy storage systems, but the unavailability of adept electrolytes has hindered their development. In this work, a range of silaborane clusters (
; n=5–15) were investigated using density functional theory (DFT) at ωB97XD3/6‐311+G(d,p) level of theory. The vertical detachment energy (VDE), electrochemical stability window (ESW) and binding energy (BE) of the silaboranes were computed at the same level of DFT. A methodology based on molecular electrostatic potential surface analysis was designed to locate the most suitable binding site for calcium ions on the clusters. DFT results show that the SiB11
cluster turns out to be superior to other candidates. Effect of substitution on silaboranes (
R− R=−CH3, −NCS, −CF3, −F and −Cl) was computed. −NCS and −CF3 substituted SiB11
ions were found to be the best from DFT. Ab initio molecular dynamics (AIMD) studies were performed to explore the interactions between silaborane‐based electrolytes and the Ca anode. AIMD results highlight the decomposition of −NCS and −CF3 substituted SiB11
on Ca anode. DFT and AIMD studies reveal that −CH3 substituted silaborane‐based Ca‐salt (Ca(SiB11H11CH3)2) is the promising electrolyte for CIBs.
Managing fuels and fluids: Network integration of osmoregulatory and metabolic hormonal circuits in the polymodal control of homeostasis in insects
Authors: Koyama Takashi , Rana Danial Wasim , Halberg Kenneth Veland 
Published: June 2023

AbstractOsmoregulation in insects is an essential process whereby changes in hemolymph osmotic pressure induce the release of diuretic or antidiuretic hormones to recruit individual osmoregulatory responses in a manner that optimizes overall homeostasis. However, the mechanisms by which different osmoregulatory circuits interact with other homeostatic networks to implement the correct homeostatic program remain largely unexplored. Surprisingly, recent advances in insect genetics have revealed several important metabolic functions are regulated by classic osmoregulatory pathways, suggesting that internal cues related to osmotic and metabolic perturbations are integrated by the same hormonal networks. Here, we review our current knowledge on the network mechanisms that underpin systemic osmoregulation and discuss the remarkable parallels between the hormonal networks that regulate body fluid balance and those involved in energy homeostasis to provide a framework for understanding the polymodal optimization of homeostasis in insects.
Changes in physical activity level of adolescents and its relationship with mental health during regular COVID‐19 prevention and control
Authors: Dong Ru‐bao , Dou Kai‐yun 
Published: June 2023

AbstractPurposeThis study aimed to analyze the impact of regular prevention and control of COVID‐19 on the physical activity level (PAL) of adolescents and the relationship between PAL and mental health.MethodsUsing the convenience sampling method, two stages of the on‐site cross‐sectional investigation were conducted in 11 middle schools in Guiyang City in China. There were 1132 participants who completed the Physical Activity Questionnaire for old children (PAQ‐C) in October 2020, and 1503 participants who completed the PAQ‐C and Mental Health Inventory of Middle‐school students (MMHI‐60) in October 2021. All participants reported their demographic data. Descriptive, quantitative statistics were used for data analysis. One‐way ANOVA was used to explore associations between PAL and mental health.ResultsThe results of statistical analysis showed a gradual yearly increase in the PAL of teenagers, and the PAL of male junior middle school students has a significant increase (p < .05); while that of adolescents in Grade 10 significantly decreased (p < .001). There is a statistically significant correlation between adolescents’ mental health (except for anxiety) and PAL (p < .05). The overall abnormal rate of mental health was 27.9%; The PAL and the total mean score of mental health had a negative correlation (p < .001). There was a significant difference between mental health scores and corresponding PAL (p < .001). Furthermore, there are statistically significant differences in scores of mental health factors corresponding to varying PAL among junior high school students and male students (p < .05).ConclusionsThe regular epidemic prevention and control measures had significant adverse effects on the PAL of girls and high school adolescents, especially Grade 10 adolescents. Improving adolescents’ PAL can promote their mental health. Interventions based on PAL slightly lower than the level recommended in the physical activity guidelines can bring significant mental health effects.
The mRNA and protein levels of the glycolytic enzymes lactate dehydrogenase A (LDHA) and phosphofructokinase platelet (PFKP) are good predictors of survival time, recurrence, and risk of death in cervical cancer patients
Authors: Bolaños‐Suárez Verónica , Alfaro Ana , Espinosa Ana María , Medina‐Martínez Ingrid , Juárez Eligia , Villegas‐Sepúlveda Nicolás , Gudiño‐Zayas Marco , Gutiérrez‐Castro América , Román‐Bassaure Edgar , Salinas‐Nieves María Eugenia , Bruno‐Muñoz Sergio , Aranda Carlos , Flores‐Herrera Oscar , Berumen Jaime 
Published: June 2023

AbstractIntroductionPatients with cervical cancer (CC) may experience local recurrence very often after treatment; when only clinical parameters are used, most cases are diagnosed in late stages, which decreases the chance of recovery. Molecular markers can improve the prediction of clinical outcome. Glycolysis is altered in 70% of CCs, so molecular markers of this pathway associated with the aggressiveness of CC can be identified.MethodsThe expression of 14 glycolytic genes was analyzed in 97 CC and 29 healthy cervical tissue (HCT) with microarray; only LDHA and PFKP were validated at the mRNA and protein levels in 36 of those CC samples and in 109 new CC samples, and 31 HCT samples by qRT–PCR, Western blotting, or immunohistochemistry. A replica analysis was performed on 295 CC from The Cancer Genome Atlas (TCGA) database.ResultsThe protein expression of LDHA and PFKP was associated with poor overall survival [OS: LDHA HR = 4.0 (95% CI = 1.4–11.1);p = 8.0 × 10−3; PFKP HR = 3.3 (95% CI = 1.1–10.5);p = 4.0 × 10−2] and disease‐free survival [DFS: LDHA HR = 4.5 (95% CI = 1.9–10.8);p = 1.0 × 10−3;PFKP HR = 3.2 (95% CI = 1.2–8.2);p = 1.8 × 10−2] independent of FIGO clinical stage, and the results for mRNA expression were similar. The risk of death was greater in patients with overexpression of both biomarkers than in patients with advanced FIGO stage [HR = 8.1 (95% CI = 2.6–26.1;p = 4.3 × 10−4) versus HR = 7 (95% CI 1.6–31.1,p = 1.0 × 10−2)] and increased exponentially as the expression of LDHA and PFKP increased.ConclusionsLDHA and PFKP overexpression at the mRNA and protein levels was associated with poor OS and DFS and increased risk of death in CC patients regardless of FIGO stage. The measurement of these two markers could be very useful for evaluating clinical evolution and the risk of death from CC and could facilitate better treatment decision making.
Efficacy and cost‐effectiveness analysis of pretreatment percutaneous endoscopic gastrostomy in unresectable locally advanced esophageal cancer patients treated with concurrent chemoradiotherapy (GASTO 1059)
Authors: Ai XinLei , Zhang PengXin , Xie XinMin , Qiu Bo , Zhu YuJia , Zhao Lei , Xi Mian , Wu YingJia , Guo SuPing , Guo JinYu , Liu FangJie , Wang DaQuan , Chen NaiBin , He QianWen , Hu YongHong , Liu MengZhong , Ding ZhaoXia , Liu Hui 
Published: June 2023

AbstractBackgroundWe launched a single‐arm phase II study to determine the efficacy and cost‐effectiveness of percutaneous endoscopic gastrostomy (PEG) before concurrent chemoradiotherapy (CCRT) in patients with esophageal squamous cell carcinoma (ESCC).MethodsEligible patients received pretreatment PEG and enteral nutrition during CCRT. The primary outcome was the change of weight during CCRT. The secondary outcome included nutrition status, loco‐regional objective response rate (ORR), loco‐regional progression‐free survival (LRFS), overall survival (OS), and toxicities. A 3‐state Markov model was applied for cost‐effectiveness analysis. Eligible patients were matched and compared with those who had nasogastric tube feeding (NTF) or oral nutritional supplements (ONS).ResultsSixty‐three eligible patients received pretreatment PEG‐based CCRT. The mean change of weight during CCRT was −1.4% (standard deviation, 4.4%), and after CCRT, 28.6% of patients gained weight and 98.4% had normal albumin levels. The loco‐regional ORR and 1‐year LRFS were 98.4% and 88.3%. The incidence of grade ≥3 esophagitis was 14.3%. After matching, another 63 patients were included in the NTF group and 63 in the ONS group. More patients gained weight after CCRT in the PEG group (p = 0.001). The PEG group showed higher loco‐regional ORR (p = 0.036) and longer 1‐year LRFS (p = 0.030). In cost analysis, the PEG group showed an incremental cost‐effectiveness ratio of $3457.65 per quality‐adjusted life‐years (QALY) compared with the ONS group with a probability of cost‐effectiveness of 77.7% at the $10,000 per QALY willingness‐to‐pay threshold.ConclusionPretreatment PEG is associated with better nutritional status and treatment outcome in ESCC patients treated with CCRT compared with ONS and NTF. Pretreatment of PEG can be cost‐effective because of its significant clinical benefits.
Systematic review of patients’ and healthcare professionals’ views on patient‐initiated follow‐up in treated cancer patients
Authors: Dretzke Janine , Lorenc Ava , Adriano Ada , Herd Clare , Mehanna Hisham , Nankivell Paul , Moore David J. ,   the PETNECK2 Research Team
Published: June 2023

AbstractBackgroundCurrent follow‐up models in cancer are seen to be unsustainable and inflexible, and there is growing interest in alternative models, such as patient‐initiated follow‐up (PIFU). It is therefore important to understand whether PIFU is acceptable to patients and healthcare professionals (HCPs).MethodsStandard systematic review methodology aimed at limiting bias was used for study identification (to January 2022), selection and data extraction. Thematic synthesis was undertaken for qualitative data, and survey findings were tabulated and described.ResultsNine qualitative studies and 22 surveys were included, mainly in breast and endometrial cancer. Women treated for breast or endometrial cancer and HCPs were mostly supportive of PIFU. Facilitators for PIFU included convenience, control over own health and avoidance of anxiety‐inducing clinic appointments. Barriers included loss of reassurance from scheduled visits and lack of confidence in self‐management. HCPs were supportive of PIFU but concerned about resistance to change, unsuitability of PIFU for some patients and costs.ConclusionPIFU is viewed mostly positively by women treated for breast or endometrial cancer, and by HCPs, but further evidence is needed from a wider range of cancers, men, and more representative samples.A protocol was registered with PROSPERO (CRD42020181412).
Cold atmospheric nitrogen plasma induces metal‐initiated cell death by cell membrane rupture and mitochondrial perturbation
Authors: Iuchi Katsuya , Fukasawa Mami , Murakami Tomoyuki , Hisatomi Hisashi 
Published: June 2023

AbstractCold atmospheric plasma (CAP) is a novel biomedical tool used for cancer therapy. A device using nitrogen gas (N2CAP) produced CAP that induced cell death through the production of reactive nitrogen species and an increase in intracellular calcium. In this study, we investigated the effect of N2CAP‐irradiation on cell membrane and mitochondrial function in human embryonic kidney cell line 293T. We investigated whether iron is involved in N2CAP‐induced cell death, as deferoxamine methanesulfonate (an iron chelator) inhibits this process. We found that N2CAP induced cell membrane disturbance and loss of mitochondrial membrane potential in an irradiation time‐dependent manner. BAPTA‐AM, a cell‐permeable calcium chelator, inhibited N2CAP‐induced loss of mitochondrial membrane potential. These results suggest that disruption of intracellular metal homeostasis was involved in N2CAP‐induced cell membrane rupture and mitochondrial dysfunction. Moreover, N2CAP irradiation generated a time‐dependent production of peroxynitrite. However, lipid‐derived radicals are unrelated to N2CAP‐induced cell death. Generally, N2CAP‐induced cell death is driven by the complex interaction between metal movement and reactive oxygen and nitrogen species produced by N2CAP.
Tuning the Electronic Structures of TMNs‐Modified Ni‐Based Catalysts to Enhance the Performance for MOR in Alkaline Media
Authors: Bai Yanxin , Xiong Hui , Jiang Zhao , Zhang Riguang , Fang. Tao 
Published: June 2023

AbstractTransition metal nitrides are considered as the promising materials in the energy conversion processes due to the similar electronic structures to Pt. In this work, a series of Ni/TMN−C (TM=V, Mo, Nb, Ta) catalysts were synthesized and investigated the catalytic performance for methanol oxidation reaction in alkaline media. The electrochemical measurements indicated that the TNNs‐modified Ni/C catalysts exhibited higher activity, kinetics, charge transfer and stability for alkaline MOR compared with Ni/C. It was found that the incorporation of TMNs improved the activity and stability for MOR in various degree. Specially, Ni/VN−C showed the highest catalytic activity, nearly three times higher than that of Ni/C due to the highest coverage of the surface component NiOOH, consistent with the Tafel and EIS results that it exhibited the fastest charge transfer kinetics and reaction rate. XPS characterization indicated that the obvious electron interaction occurred between the TMNs and Ni species and Ni would transfer electrons to the transition metal nitrides, resulting in the electron‐rich states of TMNs. Interestingly, combining the d‐band center and activity of the corresponding catalysts, it could be found that a volcano plot existed between them, suggesting that the introduction of appropriate nitrides would change the electronic structure of Ni and enhance the catalytic performance for MOR in alkaline media.
ECG compression technique using fast fractals in the Internet of medical things
Authors: Ibaida Ayman , Abuadbba Sharif , Al‐Shammary Dhiah , Khalil Ibrahim 
Published: June 2023

AbstractECG signal is widely used in most cardiology e‐health systems. Patients may be monitored continuously for at least 12 h a day. Therefore, the ECG signal size transmitted to a hospital server during continuous monitoring is significant. Furthermore, transmission of the large size ECG signal is a power consuming process. ECG compression is one of the proposed solutions to overcome this problem. In this paper, a new fractal‐based ECG lossy compression technique is proposed. It is clear that fractal can use ECG signal self similarity characteristics efficiently to achieve high compression ratios. The proposed technique is based on developing the fractal model in conjunction with Iterated Function System. Fractal is well known as a time consuming technique, and therefore, new mathematical development is proposed to potentially reduce fractal computations. Experiments have proven the significant performance of fast fractal in comparison with the traditional version. Furthermore, the resultant compression ratios are close to the traditional fractal results and higher than other existing techniques.
Development of the hippocampal CA2 region and the emergence of social recognition
Authors: Diethorn Emma J. , Gould Elizabeth 
Published: June 2023

AbstractSocial memories formed in early life, like those for family and unrelated peers, are known to contribute to healthy social interactions throughout life, although how the developing brain supports social memory remains relatively unexplored. The CA2 subregion of the hippocampus is involved in social memory function, but most literature on this subject is restricted to studies of adult rodents. Here, we review the current literature on the embryonic and postnatal development of hippocampal subregion CA2 in mammals, with a focus on the emergence of its unusual molecular and cellular characteristics, including its notably high expression of plasticity‐suppressing molecules. We also consider the connectivity of the CA2 with other brain areas, including intrahippocampal regions, such as the dentate gyrus, CA3, and CA1 regions, and extrahippocampal regions, such as the hypothalamus, ventral tegmental area, basal forebrain, raphe nuclei, and the entorhinal cortex. We review developmental milestones of CA2 molecular, cellular, and circuit‐level features that may contribute to emerging social recognition abilities for kin and unrelated conspecifics in early life. Lastly, we consider genetic mouse models related to neurodevelopmental disorders in humans in order to survey evidence about whether atypical formation of the CA2 may contribute to social memory dysfunction.
Differences in the content of human milk oligosaccharides between foremilk and hind milk
Authors: Pu Zhiping , Sun Zhaona , Liu Jun , Zhang Jinyue , Cheng Mengdi , Zhang Lina , Zhou Peng 
Published: June 2023

AbstractHuman milk oligosaccharides (HMOs) are the second largest carbohydrate component and the third largest nutrient after lactose, and their content and composition are related to the genetic characteristics of the mother, lactation period, geography, and other factors. This study examined the differences in fat content, protein composition, and oligosaccharides content between foremilk and hind milk in human milk. The results showed that the fat content of human milk was significantly higher in the hind milk than in the foremilk. However, no significant differences in the composition and content of protein in the fore and hind milk. With respect to oligosaccharides, the total amount of HMOs (based on seven oligosaccharides, lacto‐N‐neotetraose (LNnT), lacto‐N‐tetraose (LNT), 2′‐fucosyllactose (2′‐FL), 3′‐fucosyllactose (3′‐FL), 3′‐galactosyllactose (3′‐GL), 3′‐sialyllactose (3′‐SL), and 6′‐sialyllactose (6′‐SL) in the hind milk was always higher than in the foremilk in almost all mothers. Similar trend was found in specific oligosaccharide including LNnT, LNT, 2′‐FL, 3′‐FL, and 3′‐GL. On the other hand, sialylated oligosaccharides, 3′‐SL, and 6′‐SL were quite consistent in foremilk and hind milk.