bluuebunny/crossref_metadata_2025_split huggingface dataset:
A simple and cost‐effective method for studying anoxia tolerance in plants
Authors: Sherwood Orla L. , Carroll Rebecca , Burke Stephen , McCabe Paul F. , Kacprzyk Joanna 
Published: January 2023

AbstractPremiseWe developed a novel, cost‐effective protocol that facilitates testing anoxia tolerance in plants without access to specialized equipment.Methods and ResultsArabidopsis thaliana and barley (Hordeum vulgare) seedlings were treated in airtight 2‐L Kilner jars. An anoxic atmosphere was generated using Oxoid AnaeroGen 2.5‐L sachets placed on in‐house, custom‐built wire stands. The performed experiments confirmed a higher sensitivity to low oxygen stress previously observed in anac017 A. thaliana mutants and the positive effect of exogenous sucrose on anoxia tolerance reported by previous studies in A. thaliana. Barley seedlings displayed typical responses to anoxia treatment, including shoot growth cessation and the induction of marker genes for anaerobic metabolism and ethylene biosynthesis in root tissue.ConclusionsThe results validate the novel method as an inexpensive, simple alternative for testing anoxia tolerance in plants, where access to an anaerobic workstation is not possible. The novel protocol requires minimum investment and is easily adaptable.
Influence of the pKa value on the antioxidant activity of licorice flavonoids under solvent‐mediated effects
Authors: Hu Yi , Wang Zhuxian , Shen Chunyan , Jiang CuiPing , Zhu Zhaoming , Liang Peiyi , Li Hui , Zeng Quanfu , Xue Yaqi , Wu Yufan , Wang Yuan , Liu Li , Zhu Hongxia , Yi Yankui , Liu Qiang 
Published: January 2023

AbstractLicorice flavonoids (LCFs) have been widely used in food care and medical treatment due to their significant antioxidant activities. However, the molecular mechanism of their antioxidant activity remains unclear. Therefore, network pharmacology, ADMET, density functional theory (DFT), molecular docking, and molecular dynamics (MD) simulation were employed to explore the molecular mechanism of the antioxidant effects of LCF. The network pharmacology and ADMET studies showed that the active molecules of kumatakenin (pKa = 6.18), licoflavonol (pKa = 6.86), and topazolin (pKa = 6.21) in LCF are key antioxidant components and have good biosafety. Molecular docking and MD simulation studies demonstrated that active molecules interacted with amino acid residues in target proteins to form stable protein–ligand complexes and exert their antioxidant effects. DFT studies showed that the antioxidant activity of LCF could be significantly modulated under the solvent‐mediated effect. In addition, based on the derivation of the Henderson–Hasselbalch and van't Hoff formulas, the functional relationships between the reaction‐free energy (ΔG) of LCF and the pH and pKa values were established. The results showed that active molecules with larger pKa values will be more conducive to the improvement of their antioxidant activity under solvent‐mediated effects. In conclusion, this study found that increasing the pKa value of LCF would be an effective strategy to improve their antioxidant activity under the effect of solvent mediation. The pKa value of an LCF will be a direct standard to evaluate its solvent‐mediated antioxidant activity. This study will provide theoretical guidance for the development of natural antioxidants.
A review on the recent advancements in nanomaterials for nonenzymatic lactate sensing
Authors: Jannath Khatun A. , Karim Md Mobarok , Saputra Heru Agung , Seo Kyeong‐Deok , Kim Kwang Bok , Shim Yoon‐Bo 
Published: January 2023

AbstractLactate is one of the major biomarkers to assess the physical fitness of human in clinical or sports medicine. The proper and well‐timed lactate determination can avoid some exigent conditions including hemorrhage, respiratory distress, sepsis, and hypoxia. Therefore, rapid, facile, selective, and reliable detection of lactate have gained appreciation lately. Among the various lactate detection methods, electrochemical method is the most rapid, convenient, and sensitive technique. Specifically, nanomaterial‐based nonenzymatic electrochemical lactate sensors are much desirable to solve the demerits and stability issues of enzymatic lactate sensors. Numerous materials including metal and metal oxide nanoparticles, metal–organic frameworks, molecularly imprinted polymers, and carbons were used as electrocatalysts to achieve highly sensitive lactate sensors. This present review mainly focuses on the recent developments of these materials for enzyme‐free lactate oxidation and the future prospective of electrode modification catalysts for lactate sensors.
Nanoplasmonic assay platforms for reproducible SERS detection of Alzheimer's disease biomarker
Authors: Dang Hajun , Joung Younju , Jeong Chaehyeon , Jeon Chang Su , Pyun Sung Hyun , Park Sung‐Gyu , Choo Jaebum 
Published: January 2023

AbstractWith the recent developments in high‐sensitivity optical detection technologies, many studies have been conducted to accurately detect biomarkers with low concentrations of 1.0 pM or less as well as apply them to in vitro diagnostics. The tubulin‐associated unit (tau‐381) protein, a biomarker of Alzheimer's disease, is one such representative example, and its cut‐off value reported in clinical practice is 5.5 fM. Therefore, a robust sensing technology that can stably detect such low concentrations of biomarkers is needed. In this study, tau‐381 was detected with high sensitivity and reproducibility by a plasmonic Au nanopopcorn substrate fabricated via thermal evaporation. Here, aptamer DNAs labeled with Raman reporters on the terminal were used as the receptors. The plasmonic nanopopcorn substrate used in this study is composed of uniform gold nanoparticles (AuNPs) of average size 64 nm. The reproducibility was significantly improved through more uniform nanogaps than those formed by aggregation of AuNPs in solution. An assay was conducted by first reacting tau‐381 with the corresponding aptamers, and the remaining aptamer DNAs were then reacted with capture DNAs immobilized on the surface of the Au substrate. The assay results for tau‐381 showed a limit of detection value of 2.2 fM, which is below the cut‐off value (5.5 fM).
Should Sedillot's triangle be used for central venous cannulation? A cadaveric study
Authors: Dunne Henry , Abou‐Abdallah Michel , Robinson Michael , Kenyon Olivia , Brassett Cecilia 
Published: January 2023

AbstractCentral venous access remains an integral part of perioperative and intensive care, and several methods have been described to locate the internal jugular vein (IJV) prior to cannulation. The apex of Sedillot's triangle between the manubrial and clavicular heads of the sternocleidomastoid (SCM) muscle is a commonly used anatomical landmark for a central percutaneous approach to the IJV, but the literature highlights failures and complications when adopting this method. This cadaveric study was designed to investigate the usefulness of Sedillot's triangle to locate the IJV. Sixty‐one cadavers were used for investigation at the University of Cambridge Human Anatomy Centre. Sedillot's triangle was dissected and a pin was inserted in a sagittal plane at the apex of the triangle. The location of the pin in relation to the IJV was recorded. The distance between the sternal and clavicular heads of SCM was also measured. In total, the pin inserted at the apex of Sedillot's triangle pierced the IJV in 72/117 (61.5%) of dissections, with 71.4% on the right and 52.5% on the left. There was important variation in SCM anatomy, and there was no gap between its two heads in 12% of the neck dissections. We demonstrate an overall poor success rate of the central percutaneous approach using Sedillot's triangle, although our findings are limited being a simulated cadaveric study. We support education and use of ultrasound in addition to landmark techniques to aid the safe insertion of central venous catheters.
Methodology of teaching the fundamentals of electrical engineering and electronics for the pharmaceutical production technology specialty
Authors: Zhussipbekova Sholpan , Alimbekova Gulshakhan , Rystygulova Venera , Shadinova Kunsulu , Aliya Adilbekova 
Published: January 2023

AbstractThe aim of this research is to study the main pedagogical experiments conducted for the purpose of practical study of the level of mastery by students of pharmaceutical specialties of electrical engineering and electronics fundamentals. The basis of the methodological approach in this research work is a combination of existing system analysis methods of teaching disciplines related to the fundamentals of electrical engineering and electronics for nontechnical specialties of modern educational institutions, with experimental studies of pedagogical features of the perception of the taught discipline by students of pharmaceutical specialties. The results of this research indicate a weak interest of students of the pharmaceutical specialty of the Asfendiyarov Kazakh National Medical University in mastering the discipline ‘Fundamentals of Electrical Engineering and Electronics’, which indicates the need to improve existing teaching methods and develop new ones that can provide more tangible results. The results and conclusions of this research work are of significant practical importance from the standpoint of the prospects for improving the teaching of this group of disciplines for students of nontechnical specialties of educational institutions and are also of great importance for teachers of the fundamentals of electrical engineering and electronics, faced with the urgent need to teach this kind of material to students during lectures and practical classes.
Integration of Excel VBA with professional software for the structural analysis and design of civil structures
Authors: Tamayo Jorge L. P. , Mucillo Vitor C. , Bigarella Bruno G. 
Published: January 2023

AbstractA feasible combined approach between structural analysis program (SAP) 2000 and Excel software is proposed in this article to develop customized numerical tools for the analysis of civil engineering structures. For such scope, the Open Application Programming Interface from SAP 2000 software is linked to the Visual Basic for Application (VBA) environment embedded within Excel. Three computer applications resembling industrial situations related to size optimization of trusses, soil−structure interaction in buildings, and automatic design of reinforced concrete slabs are presented. As the tools are parameterized, students can observe the effects of the various parameters on the output response of the structures because geometry, loading, and material properties can be easily varied. As the main concern is the educational aspect, the novelty of this research is to present the proposed combined approach in a stepwise manner with explained codes. The source codes of the developed tools are made freely available so that the applicants can modify them according to their priorities. The tools present a friendly user interface that made them attractive to use in daily design tasks at the office, but certainly, they are the result of the integration of student knowledge as part of a bachelor's dissertation in an undergraduate civil engineering educational program. The tools have been preliminarily introduced into a reinforced concrete course at the Federal University of Rio Grande do Sul (Brazil), searching for a better understanding of theoretical concepts, while integrating technology into the classroom and reducing time constraints as new configurations for the studied structures can be easily explored during class.
Identification of EMP1 as a critical gene for cisplatin resistance in ovarian cancer by using integrated bioinformatics analysis
Authors: Zeng Qingsong , Yi Cunjian , Lu Jinzhi , Wang Xiaowen , Chen Keming , Hong Li 
Published: January 2023

AbstractBackgroundCisplatin resistance is among the main reasons for the poor prognosis of ovarian cancer (OC) patients. Until now, effective biomarkers for predicting cisplatin resistance in OC and specific drugs for reversing this resistance are lacking. This study identified the critical gene associated with cisplatin resistance in OC and provided a potential target for overcoming this resistance.MethodsDifferentially expressed genes between cisplatin‐resistant and ‐sensitive OCs were identified by screening public datasets. Survival analysis was conducted to screen prognosis‐related DEGs. CIBERSORT, ESTIMATE, and immune checkpoint genes were used to assess the association between EMP1 expression and tumor microenvironment features. CTRP and GDSC databases were employed to analyze the correlation between EMP1 expression and cisplatin resistance. Furthermore, immunohistochemistry, qPCR, Western blotting, siRNA interference, and the CCK8 assay were performed to verify the role of EMP1 in cisplatin resistance in vitro. Finally, xenograft mouse models were generated to further confirm the role of EMP1 in cisplatin resistance in vivo.ResultsEMP1 was identified as a critical gene associated with cisplatin resistance in OC. According to bioinformatics analyses, increased EMP1 expression was linked to higher stromal/ESTIMATE scores as well as greater ICG expression levels. The in vitro experiments showed that EMP1 was highly expressed in cisplatin‐resistant OC tissues and cells, and silencing this EMP1 expression enhanced OC cell sensitivity to cisplatin. Finally, in vivo experiments confirmed that EMP1 promotes tumor growth and cisplatin resistance.ConclusionsEMP1 can act as a predictive biomarker for cisplatin resistance in OC and as a potential therapeutic target.
High expression of LOC541471, GDAP1, SOD1, and STK25 is associated with poor overall survival of patients with acute myeloid leukemia
Authors: Yu Xibao , Chen Cunte , Hu Yanyun , Li Kehan , Zhang Yikai , Chen Zheng , Nie Dingrui , Gao Rili , Huang Youxue , Zhong Mengjun , Wang Caixia , Wang Shunqing , Zeng Yixin , Li Yangqiu , Zeng Chengwu 
Published: January 2023

AbstractBackgroundAcute myeloid leukemia (AML) is an aggressive heterogeneous hematological malignancy with remarkably heterogeneous outcomes. This study aimed to identify potential biomarkers for AML risk stratification via analysis of gene expression profiles.MethodsRNA sequencing data from 167 adult AML patients in the Cancer Genome Atlas (TCGA) database were obtained for overall survival (OS) analysis, and 52 bone marrow (BM) samples from our clinical center were used for validation. Additionally, siRNA was used to investigate the role of prognostic genes in the apoptosis and proliferation of AML cells.ResultsCo‐expression of 103 long non‐coding RNAs (lncRNAs) and mRNAs in the red module that were positively correlated with European Leukemia Network (ELN) risk stratification and age was identified by weighted gene co‐expression network analysis (WGCNA). After screening by uni‐ and multivariate Cox regression, Kaplan–Meier survival, and protein–protein interaction analysis, four genes including the lncRNA LOC541471, GDAP1, SOD1, and STK25 were incorporated into calculating a risk score from coefficients of the multivariate Cox regression model. Notably, GDAP1 expression was the greatest contributor to OS among the four genes. Interestingly, the risk score, ELN risk stratification, and age were independent prognostic factors for AML patients, and a nomogram model constructed with these factors could illustrate and personalize the 1‐, 3‐, and 5‐year OS rates of AML patients. The calibration and time‐dependent receiver operating characteristic curves (ROCs) suggested that the nomogram had a good predictive performance. Furthermore, new risk stratification was developed for AML patients based on the nomogram model. Importantly, knockdown of LOC541471, GDPA1, SOD1, or STK25 promoted apoptosis and inhibited the proliferation of THP‐1 cells compared to controls.ConclusionsHigh expression of LOC541471, GDAP1, SOD1, and STK25 may be biomarkers for risk stratification of AML patients, which may provide novel insight into evaluating prognosis, monitoring progression, and designing combinational targeted therapies.
Comparison of the prognosis of symptomatic cerebral infarction and pulmonary embolism in patients with advanced non‐small cell lung cancer
Authors: Nakamura Ryota , Yamada Tadaaki , Tanaka Satomi , Sasada Aosa , Shiotsu Shinsuke , Tani Nozomi , Takeda Takayuki , Chihara Yusuke , Hirai Soichi , Takemura Yoshizumi , Yoshimura Akihiro , Morimoto Kenji , Iwasaku Masahiro , Tokuda Shinsaku , Kim Young Hak , Takayama Koichi 
Published: January 2023

AbstractBackgroundLung cancer patients face a high risk of thromboembolism (TE), which is considered to be a poor prognostic factor. However, the impact of symptomatic cerebral infarction (CI) and pulmonary embolism (PE) on the prognosis of advanced non‐small cell lung cancer (NSCLC) patients is not fully understood.MethodsWe retrospectively identified 46 patients with advanced NSCLC who developed symptomatic CI or PE at five hospitals in Japan between January 2010 and December 2019. Prognosis and biomarker levels after incident CI and PE were investigated.ResultsOf the 46 patients, 36 developed symptomatic CI, and 10 developed symptomatic PE. The median follow‐up duration after incident CI and PE was 18.2 months. Although the proportion of Common Terminology Criteria for Adverse Events grade 4 tended to be higher in patients with PE than in those with CI (30% vs. 11%, p = 0.16), the overall survival (OS) after incident TE tended to be worse in patients with CI than in those with PE (median 2.3 months vs. 9.1 months, log‐rank test p = 0.17). Multivariate analysis showed that OS after CI was worse in patients with high D‐dimer (DD) levels than in those with low DD levels at the time of incident CI (median 1.3 months vs. 8.3 months, log‐rank p < 0.001).ConclusionsThis retrospective study demonstrated that the prognosis of patients tended to be poorer after CI than after PE. The DD levels at the time of incident CI might be a promising predictor of clinical outcomes in advanced NSCLC patients who develop CI.
Assessment of survival outcomes among lung cancer patients at the National and Referral Hospital in Kenya
Authors: Said Nur Swaleh , Degu Amsalu 
Published: January 2023

AbstractIntroductionLung cancer has a low overall survival rate linked to late diagnosis and metastasis. Unfortunately, comprehensive data within the African continent are limited due to the lack of a registry, low public awareness of lung cancer, financial constraints, and inadequate screening and treatment facilities. In addition, there was a lack of conclusive data in our setting. Therefore, this study aimed to assess survival outcomes among lung cancer patients at Kenyatta National Hospital.MethodsA hospital‐based retrospective cohort study was performed to examine the survival outcomes of 151 lung cancer patients. All eligible lung cancer patients diagnosed and treated in the facility between January 1, 2018, and December 31, 2020, were included. The patients were retrospectively followed from the date of primary cancer diagnosis until death or the last follow‐up period. The Statistical Package for the Social Sciences (SPSS) version 20.0 statistical software was used to enter and analyze the data. Kaplan–Meier survival and Cox regression analysis were employed to determine median survival and predictors of mortality, respectively.ResultsThe mean and median follow‐time was 18.2 and 17.5 months, respectively. Most (98%) of the patients had non‐small cell lung cancer. The 2‐year survival rate was 66.7%, with 59.6% of patients having developed distant metastasis during the follow‐up, while 25.1% were deceased. The median cancer‐specific survival time among the study population was 18.0 ± 3.40 months. Cox regression analyses showed that patients with distant metastasis had five times more risk of dying (AHR: 4.74, 95% CI: 2.1–10.8, p < 0.001) than patients without distant metastasis.ConclusionsThe overall two‐year survival rate of lung cancer patients at the Kenyatta National Hospital was 66.7%, with most patients developed distant metastasis during the follow‐up period. Distant metastasis was the only significant predictor of mortality among lung cancer patients in our setting.
Challenges in management of Bartholin gland leiomyoma: A case report
Authors: Vinturache Angela Elena , Ismail Lamiese , Damato Stephen , Soleymani Majd Hooman 
Published: January 2023

AbstractLeiomyomas are uncommon vulvar neoplasms often misdiagnosed as other Bartholin gland pathology. This case report describes a case of accelerating growth of a vulvar mass, initially diagnosed as Bartholin cyst. Surgical excision led to a histopathologic diagnosis of vulvar leiomyoma. The postoperative recovery was complicated by secondary hematoma and dehiscence of the surgical site. There was no recurrence at 2 years follow‐up.
Rare presentation of retroperitoneal leiomyosarcoma mimicking a myoma in a 46‐year‐old woman: A case report
Authors: Akhavan Setareh , Sheikhhasani Shahrzad , Peydayesh Mohades , Alizadeh Shima , Zamani Fatemeh , Zamani Narges 
Published: January 2023

AbstractRetroperitoneal sarcoma is relatively uncommon. We share our experience in encountering retroperitoneal sarcoma with vascular and urethral adhesion in a 46‐year‐old woman. Given the rarity of these tumors, evaluation and management should ideally be performed in a center equipped with multidisciplinary expertise in treating sarcomas.
Self‐Supporting Iron‐Modified Nickel Phosphide Electrode Realizing Superior Bifunctional Performance for Water Splitting
Authors: Chen Pengzuo , Li Kaixun , Ye Yutong , Wu Doufeng , Tong Yun 
Published: January 2023

AbstractUtilizing non‐noble metal‐based materials to design highly active and low‐cost bifunctional electrocatalysts play a vital role in oxygen evolution reaction (OER) and hydrogen evolution reaction (HER) for the overall water splitting. Herein, a self‐supporting nickel phosphide electrode with the decoration of FeOOH species is successfully prepared by a simple electrodeposition and maceration drying process. This kind of electrode exhibits superior bifunctional activity for both HER and OER. Benefiting from the more exposed catalytic active sites, open hierarchical structure, and better reaction kinetics. At the current density of 100 mA cm−2, the optimal Ni2P−FeOOH/NF delivers the small overpotentials of 200 mV and 235 mV for HER and OER in an alkaline solution, respectively. After further assembling into the electrolyzer, the Ni2P−FeOOH/NF‐based device shows a lower cell voltage of 1.5 V and 1.66 V to reach the current density of 10 mA cm−2 and 100 mA cm−2, along with superior stability. This work outlines a promising strategy for the facile preparation of self‐supporting electrodes for practical application in hydrogen generation by overall water splitting.