bluuebunny/crossref_metadata_2025_split huggingface dataset:
P24 Emollient use and effects on cutaneous allergen reactions: a preliminary study
Authors: Katibi Oludolapo Sherifat , Andrew Paul , Poyner Anna  , Pinnock Abigail , Brown Kirsty , Byers Rob  , Cork Michael , Danby Simon 
Published: July 2023

Abstract
Recently, there has been some evidence of an increased incidence of food allergy in infants regularly applying some types of emollients. Considering that emollient vehicles can modify the delivery of ‘actives’ into the skin, it is important to examine their effects on epicutaneous sensitization to environmental allergens. The aim was to determine the effects of regular emollient use on cutaneous reactions to house dust mite allergen. An observer-blind within-participant multilateral controlled interventional study in 40 adults with confirmed house dust mite allergy was carried out. Participants underwent 4 weeks of treatment with olive oil, coconut oil and a basic emollient cream (no added moisturizers) applied to three different areas of both forearms with a fourth area left as a no-treatment control (NTC). At the end of treatment, skin barrier integrity was assessed by performing transepidermal water loss (TEWL) measurements in conjunction with tape-stripping. At adjacent locations, atopy patch tests (APTs) using house dust mite allergen were performed. This interim analysis includes data from the first 16 participants. Skin barrier integrity was reduced by all three treatments relative to NTC as indicated by elevated TEWL [mean (SD) olive oil 35.10 (15.17), coconut oil 36.56 (17.17) and emollient cream 39.18 (20.91) vs. NTC 28.76 (13.06) g m–2 h–1; P &lt; 0.05]. Pretreatment of the skin had a significant effect on APT reactions (P = 0.0401, graded from 0 to 4). The strongest reactions were seen following pretreatment with emollient cream [2.31 (1.12)], olive oil [2.22 (0.91)] and coconut oil [1.94 (0.91)] vs. NTC [1.50 (0.80)]. These preliminary results suggest that some commonly used emollients decrease skin barrier integrity and increase the severity of reactions to house dust mite allergens. This implies that regular use of emollients that damage the skin may directly affect allergy development arising from epicutaneous sensitization. Some sophisticated emollients (with moisturizers) have been shown to improve skin barrier function. Clinical trials are therefore needed to determine if they impart protection against epicutaneous sensitization.
P27 Uncovering the roles of lysosomal protease cathepsin H in atopic dermatitis
Authors: Tachie-Menson Theresa , Azizah Atqiya , Hughes Aaron  , O'Shaughnessy Ryan 
Published: July 2023

Abstract
Atopic dermatitis (AD) is a persisting skin condition characterized by recurring eczematous lesions and pruritus. It is ranked as a nonfatal disease with the fifteenth-highest global burden. The causes of AD are diverse: a defective epidermal barrier, epidermal inflammation, an altered epidermal microbiome and genetics. However, much of the treatment for AD focuses on reducing inflammation. Further understanding on how the epidermal barrier maintains its integrity may be useful in developing treatments that specifically help to restore the defective epidermal barrier. We have identified cathepsin H, a lysosomal protease with aminopeptidase activity, as a protease with key roles in maintaining the integrity of the epidermal barrier. Ctsh+/– mice exhibit reduced corneocyte integrity, a reduction in the expression of the cornified envelope protein loricrin, reduced filaggrin processing and altered skin immunity, among other defects. These highlight the significant roles of cathepsin H within the epidermal barrier and terminal differentiation process of epidermal keratinocytes. To investigate the roles of cathepsin H in the epidermal barrier we have used patient biopsies, patient tape-strip samples and rat epidermal keratinocytes (REKs), a keratinocyte cell line that spontaneously undergoes differentiation and recapitulates the complete differentiation process of human epidermal keratinocyte. Using REKs, we have generated Ctsh knockdown cell lines, from which we have derived three-dimensional organotypic cultures, as well as cathepsin H and cathepsin B stable overexpression cell lines. Moreover, we have performed mass spectrometry analysis of tape-strip samples from patients with AD exhibiting a high or low expression of cathepsin H. Our research aims to uncover the protein targets of cathepsin H in the epidermis and its ensuing functions in the epidermal barrier.
P33 Investigating the utility and exploring biological targets of micro-wound healing compounds in 2D and 3D skin models
Authors: Gorkic Metka , Messenger David , Wainwright Linda  , Connelly John , Bishop Cleo L   
Published: July 2023

Abstract
An injury to the epidermis can result in a micro-wound, characterized by its localization above the dermal–epidermal junction and an absence of vessel damage. Re-epithelialization is driven by keratinocytes, which organize into overlapping migrating and proliferating zones to ensure faithful micro-wound closure. Although still relatively unexplored, keratinocyte-mediated cytokine secretion is thought to be a feature of the micro-wound re-epithelialization response. A failure to re-epithelize, also characteristic of chronic wounds, can lead to reoccurring infections and hyperpigmentation. Although wound healing has been extensively researched, there are gaps in our understanding of the cellular mechanisms involved in the onset and completion of re-epithelization, leading to a scarcity of available enhanced micro-wound healing compounds (MHCs). To investigate the utility of a panel of putative MHCs, a new high-throughput scratch assay method was devised. A guide for multichannel pipette scratches was produced by three-dimensional printing an autoclavable 96-well plate lid with custom-made lengthwise slits. Using an immortalized keratinocyte cell line (N/TERT), the rate of scratch healing was characterized. Critical re-epithelization time points have been determined, and markers of migration are being explored within the identified periods. Moreover, known keratinocyte migration-inducing compounds are being tested to serve as a positive control for enhanced MHC screening. Any putative MHC hits will be further explored using proteomic approaches to elucidate the signalling pathways involved in the onset and completion of re-epithelization. This analysis will assist in the identification of novel enhanced MHC targets and further our understanding of keratinocyte re-epithelization.
Troubled and Troublesome Teenagers: Towards Critical and Relationship-based Practice
Authors: McDonald Lisa , Rogowski Steve 
Published: July 2023

AbstractIn neoliberal and associated managerial times, social work in the UK is dominated by the speedy completion of bureaucracy to ration services and assess/manage risk. Concerning children and families, unless there are child protection issues little help or support is provided with parents/carers often told to change their behaviour/lifestyle or face the possibility of losing their children to adoption. To counteract this, we argue for critical and relationship-based practice, using work with troubled and troublesome teenagers as an example. Such practice involves a broad responsibility to seek equality and social justice as well as address individual problems and difficulties. Importantly, the article includes the perspective of someone who has the lived and care experience of being a troubled/troublesome teenager.
Dissociated amplitude and phase effects of alpha oscillation in a nested structure of rhythm- and sequence-based temporal expectation
Authors: Su Zhongbin , Zhou Xiaolin , Wang Lihui 
Published: July 2023

Abstract
The human brain can utilize various information to form temporal expectations and optimize perceptual performance. Here we show dissociated amplitude and phase effects of prestimulus alpha oscillation in a nested structure of rhythm- and sequence-based expectation. A visual stream of rhythmic stimuli was presented in a fixed sequence such that their temporal positions could be predicted by either the low-frequency rhythm, the sequence, or the combination. The behavioral modeling indicated that rhythmic and sequence information additively led to increased accumulation speed of sensory evidence and alleviated threshold for the perceptual discrimination of the expected stimulus. The electroencephalographical results showed that the alpha amplitude was modulated mainly by rhythmic information, with the amplitude fluctuating with the phase of the low-frequency rhythm (i.e. phase-amplitude coupling). The alpha phase, however, was affected by both rhythmic and sequence information. Importantly, rhythm-based expectation improved the perceptual performance by decreasing the alpha amplitude, whereas sequence-based expectation did not further decrease the amplitude on top of rhythm-based expectation. Moreover, rhythm-based and sequence-based expectations collaboratively improved the perceptual performance by biasing the alpha oscillation toward the optimal phase. Our findings suggested flexible coordination of multiscale brain oscillations in dealing with a complex environment.
Menstrually-related migraine shapes the structural similarity network integration of brain
Authors: Li Xinyu , Hao Huifen , Li Yingying , Au Lisa Wing-Chi , Du Ganqin , Gao Xiuju , Yan Junqiang , Tong Raymond Kai-Yu , Lou Wutao 
Published: July 2023

Abstract
Menstrually-related migraine (MM) is a primary migraine in women of reproductive age. The underlying neural mechanism of MM was still unclear. In this study, we aimed to reveal the case–control differences in network integration and segregation for the morphometric similarity network of MM. Thirty-six patients with MM and 29 healthy females were recruited and underwent MRI scanning. The morphometric features were extracted in each region to construct the single-subject interareal cortical connection using morphometric similarity. The network topology characteristics, in terms of integration and segregation, were analyzed. Our results revealed that, in the absence of morphology differences, disrupted cortical network integration was found in MM patients compared to controls. The patients with MM showed a decreased global efficiency and increased characteristic path length compared to healthy controls. Regional efficiency analysis revealed the decreased efficiency in the left precentral gyrus and bilateral superior temporal gyrus contributed to the decreased network integration. The increased nodal degree centrality in the right pars triangularis was positively associated with the attack frequency in MM. Our results suggested MM would reorganize the morphology in the pain-related brain regions and reduce the parallel information processing capacity of the brain.
GC Analysis of Metformin, Ranitidine and Famotidine from Pharmaceuticals and Human Serum
Authors: Zounr Rizwan A , Khuhawar Muhammad Y , Khuhawar Taj M J , Lanjwani Muhammad F , Khuhawar Muzamil Y 
Published: July 2023

Abstract
A method has been designed based on gas chromatography with flame ionization detection (FID) for the separation and analyses of ranitidine, famotidine and metformin after pre-column derivatization with trifluoroacetylacetone and ethyl chloroformate. DB-1 (30 m × 0.32 mm id) column with film thickness 0.25 μm was used for the separation at an initial temperature of column was 100°C for 2 min, and ramping at 20°C/min up to 250°C, with a hold time of 3 min. The rate of nitrogen flow was 2.5 mL/min and FID was used for detection. Complete separation was obtained between all the three drugs including excess of derivatization reagents. Linear calibration curves and detection limits were obtained in the ranges 0.1–30 μg/mL and 0.011–0.015 μg/mL. The procedure was repeatable in terms of peak heights/peak areas and retention time (n = 5) for derivatization, quantitation and separation with relative standard deviations (RSDs) within 2.0–3.0%. The approach was examined for the analyses of drug products and serum after the intake of the drugs by healthy volunteers, and recoveries were obtained within 95–98% with RSDs 2.4–3.1%.
Bioimaging of glucose analogs labeled at the C-1 or C-2 position with a fluorescent dansylamino group
Authors: Yanagida Mio , Nakano Hirofumi , Ueno Hironori 
Published: July 2023

Abstract
Glucose is the most important energy source in all organisms; however, our understanding of the pathways and mechanisms underlying glucose transportation and localization in living cells is incomplete. Here, we prepared two glucose analogs labeled with a dansylamino group at the C-1 (1-Dansyl) or C-2 (2-Dansyl) position; the dansyl group is a highly fluorescent moiety that is characterized by a large Stokes shift between its excitation and emission wavelengths. We then examined the cytotoxicity of the two glucose analogs in mammalian fibroblast cells and in the ciliated protozoan Tetrahymena thermophila. In both cell types, 2-Dansyl had no negative effects on cell growth. The specificity of cellular uptake of glucose analogs was confirmed using an inhibitor of glucose transporter in NIH3T3 cells. In NIH3T3 cells and T. thermophila, fluorescence microscopy revealed that the glucose analogs localized throughout the cytoplasm, but especially at the periphery of the nucleus. In T. thermophila, we also found that swimming speed was comparable in media containing non-labeled glucose or one of the glucose analogs, which provided more evidence not only that the analogs were not cytotoxic in these cells but also that the analogs had no negative effect on the ciliary motion. Together, the present results suggest that the glucose analogs have low toxicity and will be useful for bioimaging of glucose-related systems.
Unequal racial distribution of immunotherapy for late-stage non–small cell lung cancer
Authors: Chang Annie , Flores Raja M , Taioli Emanuela 
Published: July 2023

Abstract
Immunotherapy has increased survival for non–small cell lung cancer (NSCLC), especially for those diagnosed with late-stage disease. However, it is not known if its use is equally distributed across races. We assessed immunotherapy use in 21 098 pathologically confirmed stage IV NSCLC patients according to race in the Surveillance Epidemiology, and End Results–Medicare linked dataset. Multivariable models were conducted to evaluate the independent association of receipt of immunotherapy with race and overall survival according to race. Black patients had statistically significantly lower odds of receiving immunotherapy (adjusted odds ratio = 0.60, 95% confidence interval = 0.44 to 0.80); receipt of immunotherapy was lower in Asian and Hispanic patients but not statistically significant. When immunotherapy was received, survival was similar across races. Immunotherapy for NSCLC is not used equally among races, underscoring the racial disparities that exist in access to the newest cancer treatment. Efforts should be directed toward expanding access to novel, efficacious treatments for advanced stage lung cancer.
(165) PAROXETINE COMBINED WITH ATLAS987 DOES NOT INHIBIT SEXUAL BEHAVIOR IN SEROTONIN TRANSPORTER KNOCKOUT RATS
Authors: Prof. Jocelien D Olivier , Jolijn Hogenbirk , Stephen Stephen De Prêtre , Prof. Berend Olivier 
Published: July 2023

Abstract

Objectives
Enduro is a drug that combines paroxetine with the 5-HT1A-receptor antagonist Atlas987. In rats this drug successfully acutely inhibits the sexual behavior, making it relevant for on demand treatment of lifelong premature ejaculation in men. Because the serotonin transporter promoter region (5-HTTLPR) polymorphism is associated with the latency to ejaculate in men with premature ejaculation, we were interested to test this drug in an animal model without the serotonin transporter, the serotonin transporter knockout (SERT-/-) rat. We hypothesized that SERT-/- rats would not respond to an SSRI, but that the 5-HT1A-receptor antagonist Atlas987 would inhibit sexual behavior as previously shown with the reference 5-HT1A-receptor antagonist WAY100635.

Methods
Wildtype (SERT+/+) and SERT-/- male Wistar rats were trained in a 30-minute weekly sexual performance test, for ten weeks. Rats with a high number of ejaculations (10 SERT+/+ and 10 SERT-/-) were selected. Atlas987 (s.c.) was dose-dependently administered with either placebo (saline) or Paroxetine (10 mg/kg; i.p.). Acute responses on sexual behavior assessed in both SERT+/+ and SERT-/- rats.

Results
A clear reduction in the number of ejaculations was found in SERT-/- rats compared with SERT+/+ rats. As shown before, the combination of Atlas987 and paroxetine acutely reduced the number of ejaculations in wildtype rats. No effects of paroxetine and Atlas987 were found in SERT-/- rats. In addition, Atlas987 alone did not influence the number of ejaculations in both SERT+/+ and SERT-/- rats. This suggests that the 5-HT1A receptor involved with sexual behavior is desensitized in SERT-/- animals.

Conclusions
Enduro successfully inhibited sexual behavior in SERT+/+ rats, but had no effects on SERT-/- rats. Knocking out the serotonin transporter gene probably decreased the sensitivity to a 5-HT1A antagonist in sexual behavior.

Conflicts of Interest
All authors declare to have no conflicts of interest.
(145) VITAMIN D DEFICIENCY IS ASSOCIATED WITH SEXUAL FUNCTION IMPAIRMENT: FINDINGS FROM A CROSS SECTIONAL STUDY
Authors: Dr. Luca Boeri , Dr. Edoardo Pozzi , Dr. Federico Belladelli , Dr. Giuseppe Fallara , Dr. Simone Cilio , Dr. Francesco Lanzaro , Dr. Alessandro Bertini , Dr. Luigi Candela , Massimiliano Raffo , Fausto Negri , Alessia d'Arma , Ludovica Cella , Margherita Fanton , Dr. Christian Corsini , Dr. Paolo Capogrosso , Prof. Francesco Montorsi , Prof. Andrea Salonia 
Published: July 2023

Abstract

Objectives
Vitamin D (vitD) is an antioxidant molecule involved in endothelial protection. Previous studies have investigated the association between vitD and erectile dysfunction (ED) with conflicting findings. We aimed to investigate the association between vitD levels and erectile function in a cohort of men seeking medical help for ED as their primary compliant.

Methods
Data from 540 men were analyzed. Comorbidities were scored with the Charlson Comorbidity Index (CCI). Patients completed the International Index of Erectile Function (IIEF) and the Beck Inventory for Depression (BDI). Circulating hormones and VitD levels were measured in every patient [deficiency for vitD was defined as &lt;20 ng/mL (group A); vitD insufficiency for vitD levels between 20 and 30 ng/mL (group B); normal level for vitD &gt;30 ng/ml (Group C)]. Descriptive statistics and linear regression models tested the association between clinical variables and sexual functioning.

Results
Overall, 211 (39.1%), 197 (36.5%) and 132 (24.4%) patients were in group A, B and C, respectively. Groups were similar for age, BMI, CCI, sex steroids, smoking and alcohol habits. IIEF-EF domain score was lower in group A compared to group B (p&lt;0.01) and group C (p&lt;0.01). Severe ED was more frequently observed in group A men than the other groups [47.5% vs. 29.2% vs. 23.3% for group A, B and C, respectively (p=0.03)]. Group A patients reported lower scores of IIEF-sexual desire (p=0.03) and IIEF-overall satisfaction (p&lt;0.01) domains than those in group C. BDI score was comparable among groups. At multivariable linear regression analysis younger age (beta -0.1, p=0.01), lower CCI (beta -1.3, p=0.04) and BDI scores (beta -0.2, p&lt;0.001) and vitD deficiency (beta -2.5, p=0.02) were significantly associated with IIEF-EF, after accounting for total testosterone and smoking status.

Conclusions
VitD deficiency is associated with overall sexual function impairment. The association between vitD and EF is even independent from clinical characteristics.

Conflicts of Interest
No conflicts of interest to declare.
(213) TESTOSTERONE SUPPLEMENTATION AND BONE PARAMETERS: A SYSTEMATIC REVIEW AND META-ANALYSIS STUDY
Authors: Dr. Walter Vena , Prof. Giovanni Corona , Dr. Alessandro Pizzocaro , Dr. Vito Angelo Giagulli , Dr. Davide Francomano , Dr. Giulia Rastrelli , Prof. Gherardo Mazziotti , Dr. Alessandro Brunetti , Prof. Antonio Aversa , Dr. Andrea Isidori , Prof. Rosario Pivonello , Prof. Linda Vignozzi , Edoardo Mannucci , Prof. Mario Maggi , Prof. Alberto Ferlin 
Published: July 2023

Abstract

Objectives
The role of testosterone (T) replacement therapy (TRT) in subjects with late onset hypogonadism is still the object of an intense debate.

Methods
All observational studies and placebo-controlled or -uncontrolled randomized trials (RCTs) comparing the effect of TRT on different bone parameters were considered.

Results
Out of 349 articles, 36 were considered, including 3103 individuals with a mean trial duration of 66.6 weeks. TRT improves areal bone mineral density (aBMD) at the spine and femoral neck levels in observational studies, whereas placebo-controlled RTCs showed a positive efect of TRT only at lumber spine and when trials included only hypogonadal patients at baseline (total testosterone&lt;12 nM). The effects on aBMD were more evident in subjects with lower T levels at baseline and increased as a function of trial duration and a higher prevalence of diabetic subjects. Either T or estradiol increase at endpoint contributed to aBMD improvement. TRT was associated with a signifcant reduction of bone resorption markers in observational but not in controlled studies.

Conclusions
TRT is able to inhibit bone resorption and increase bone mass, particularly at the lumbar spine level and when the duration is long enough to allow the anabolic efect of T and estrogens on bone metabolism to take place.

Conflicts of Interest
The authors declare no confict of interest.
(196) A MORPHOMETRIC ANALYSIS OF THE PENIS BEFORE AND AFTER PROSTAGLANDIN INJECTION: IS “SHOWER” AND “GROWER” EVEN A THING
Authors: Dr. Manuel Alonso-Isa , Dr. Borja García-Gómez , Dr. Guillermo Celada-Luis , Dr. Alessandro Fiorillo , Dr. Daniel Tueti-Silva , Dr. Esther García-Rojo , Eduard García-Cruz , Javier Romero-Otero 
Published: July 2023

Abstract

Objectives
The colloquial terms “grower” and “shower” refer to those men whose penis change significantly without or with erection, and those whose penis don't. We aim to analyze the morphometric changes in the penis after prostaglandin injection, detect if there are different patterns and which factors might be related to those patterns.

Methods
The retrospectively review a total of 225 patients from February 2021 to October 2022 who underwent penile ultrasonography with prostaglandin injection (10 mcg). Demographic, vascular and other relevant variables were recorded for analysis. A history of Peyronie disease or penile surgery (excluding postectomy and frenuloplasty) were exclusion criteria. We measured penile length (in cm) and tunica albuginea thickness (in mm) in flaccid and erect state. Only grade 3 of Erection Hardness Score (EHS) and EHS 4 were included.

Results
Mean age 48 (range 17-98). Mean penis size (flaccid) 10.07cm (range 4-17). Mean SFLP (stretch flaccid penile length) 15.32 cm (range 10.4-19.7). Mean EPL (erect penile length) 14.37 cm (range 9-19.5)
Median penile growth (PG) 42.6 % (range 3.57-264.1). PG has a normal distribution, based on 1 SD:
PG&lt;30.94% can be considered “shower”.
PG &gt;55.73% can be considered “grower”.
Intermediate results would be the “grey area”.
“Growers” have a thinner albuginea (0,9 mm; p &lt; 0,05) and a greater albuginea reduction (28%; p &lt; 0,05) in erection compared to “showers” (1,2 mm and 16,2%) respectively.

Conclusions
We have established growth ranges from which we can consider a penis grower, shower or grey zone. The size of the albuginea and its reduction during erection is related to the growth of the erect penis.

Conflicts of Interest
Authors have no potential conflict of interest to report.