bluuebunny/crossref_metadata_2025_split huggingface dataset:
A Multi-Omics Analysis of the Prognostic Biomarker GDF15 Associated with Immune Infiltration and Regulated Mitochondrial-Dependent Apoptosis in Colorectal Cancer
Authors: Wu Xiaoyu , Cao Qinhong , Zhu Yimiao , Yang Gang , Yang Hao , Wu Wenya , Sun Dongdong 
Published: September 2023

Abstract

Background:Growth differentiation factor 15 (GDF15), also known as NSAID activated gene-1 (NAG-1), is associated with a large number of biological processes and diseases, including cancer. However, the correlation between the use of GDF15 and the development and immune infiltration of colorectal cancer (CRC) has rarely been studied.Methods:The expression level of GDF15 in pan-cancer was examined using the TIMER 2.0 and TCGA databases. Using the TCGA database and GEPIA, the potential utility of GDF15 as a diagnostic and prognostic biomarker in pan-cancer was assessed. Analysis of the relationship between GDF15 and immune infiltration in CRC was performed using the ESTIMATE method. To examine the level of GDF15 expression in CRC tissues and CRC cell lines, Western blot analysis and qRT‒PCR were carried out. The ability of CRC cells to proliferate, migrate, and invade was examined using colony formation, Transwell assays, and EdU tests. To examine how GDF15 affects the cell cycle and apoptosis, flow cytometry and JC-1 assays were employed.Results:Both CRC and most malignancies had high overexpression of GDF15. In most malignancies, higher expression levels of GDF15 were linked to worse overall survival (OS) and disease-free survival (DSS), according to Kaplan‒Meier survival analyses. The expression of GDF15 was also found to be negatively connected with tumor immune infiltration, as well as with CD4+ T cells, CD8+ T cells, and neutrophil infiltration, in the majority of malignancies. According to thein vitroresearch, DGF15 levels were higher in CRC tissues and cell lines. Additionally, GDF15 knockdown markedly reduced CRC cell motility, proliferation, and G0/G1 arrest. Most importantly, we also observed that downregulation of GDF15 can promote mitochondrial-related apoptosis in CRC cells.Conclusions:In multiple human cancers, particularly in CRC, GDF15 overexpression predicts progression and a poor prognosis. GDF15 may also be an entirely novel diagnostic marker and offer a possible immune treatment target for tumor immunotherapy.
Complete submental single-port suspended endoscopic thyroid cancer surgery
Authors: Xing Zhiwei , Yu Gaoyuan , Wu Delin , Pan Tingting , Wang Zikang , Tao Gongshuai , Rao Liangmi , Ma Xiaopeng , Geng Rui 
Published: September 2023

Abstract
Background
Our team has developed a novel surgical method called 'Completely Submental Single-Port Suspended Thyroid Cancer Surgery' based on various endoscopic thyroid surgery techniques. Additionally, we have created a new set of endoscopic instruments for submental single-port suspended endoscopic thyroidectomy. In this essay, we present our initial experience with a new type of endoscopic thyroid device and evaluate its effectiveness and safety.
Methods
This study reports on the use of a novel endoscopic thyroidectomy instrument for performing submental single-port suspended endoscopic thyroidectomy. We summarize our experience with the procedure and present the collected clinical data.
Results
Of the 71 eligible patients underwent thyroid gland lobectomy (55 women [77.5%]; median [IQR] age, 35 [29–41] years), with 64 having a unilateral procedure and 7 having bilateral. Of the total, 7 patients experienced postoperative hoarseness of the voice, which resolved within 3 months. One patient experienced transient hypoparathyroidism, while 3 patients reported subjective sensations of numbness or tingling in the neck.
Conclusion
Submental single-port suspended endoscopic thyroidectomy is a safe and feasible surgical treatment option, especially when combined with new endoscopic thyroid instrumentation.
The ZcVgR gene is involved in the regulation of reproduction and lifespan in female Zeugodacus cucurbitae (Coquillett) mediated by short-term high temperature
Authors: Lian Yuyang , Yang Shuyan , Peng Sihua , Wang Aqiang , Jia Jingjing , Wang Bao , Chen Junyu , Feng Xuejie , Wu Qianxing , Zheng Lijiu , Ji Xuncong , Yang Xiaofeng , Liao Jianjun , Wei Wei , Zhuang Ling , Mai Mingxing , Zhou Shihao 
Published: September 2023

Abstract
Zeugodacus cucurbitae (Coquillett) is an important pest of fruit and vegetable crops. Previous studies have shown that differential expression of the vitellogenin receptor (ZcVgR) gene mediated by short-term high temperature causes a decrease in egg number and lifespan in female Z. cucurbitae. To comprehensively understand the molecular mechanism of the ZcVgRgene in the regulation of reproduction and lifespan, Illumina high-throughput sequencing and Tandem Mass Tags (TMT) quantitative proteomics methods were used to determine the transcriptional and proteomic information of female Z. cucurbitae after silencing the target gene (ZcVgR) by siRNA in this paper. The results showed that after silencing the target gene, the expression of the ZcVgRgene was significantly downregulated at the transcriptional level, there was no significant difference at the protein level, and the correlation between the transcriptome and proteome was low; silencing the ZcVgRgene resulted in the downregulation of vitellogenin-1 (ZcVg1), juvenile hormone epoxide hydrolase (JHEH), troponin C (TnC), heat shock protein 70 (HSP70) and other related genes at the transcriptional level, which reduced egg number and heat tolerance in adult females, leading to reduced reproductive capacity and short lifespan; silencing the ZcVgR gene activated immune-related pathways and inhibited energy metabolism-related pathways at the transcriptional level; activated Glycometabolism and Phagosome pathways at the protein level, and inhibited Phototransduction-fly and Autophagy-animal pathways. The results of this study may provide a theoretical basis for integrated control of Z. cucurbitae during the hot season.
A Case Report of Gelatinous Transformation of Bone Marrow Following Lymphoma and a Novel, Potential Treatment
Authors: Hahn Logan , Torlakovic Emina , Bosch Mark 
Published: September 2023

Abstract
Background
Gelatinous transformation of bone marrow (GTBM) is a rare hematologic condition in which hematopoietic cells in the bone marrow are replaced by extracellular gelatinous substances, often resulting in cytopenias. The true incidence of this condition is presently unknown, as the current body of literature primarily consists of case reports. However, an analysis of a large bone marrow registry suggests that this is a highly rare entity even amongst a population requiring bone marrow biopsy.
Case Presentation:
We hereby present a case of a 24-year-old man with a history of diffuse-large B-cell lymphoma (DLBCL) and an associated 45-kilogram weight loss who was found to have GTBM. The extent of his cytopenias resulted in a prolonged hospitalization with numerous complications, eventually leading to experimental treatment with allogeneic stem cell transplantation (ASCT).
Conclusions
To our knowledge, this is the first reported case of GTBM in which ASCT was employed as a potential treatment modality. While our patient did have clinical improvement following ASCT, the permanence of these results is presently unclear. Furthermore, it is uncertain if the ASCT was truly causative of the stabilization of the patient. Given this, we are currently unable to advocate for ASCT as a treatment for GTBM. We suggest that clinicians consider GTBM as a diagnostic possibility in patients with refractory cytopenias.
The association between hepatic viral infections and cancers: A cross-sectional study in the Taiwan adult population
Authors: Maliko Moreen , Su Fu-Hsiung , Kamiza Abram Bunya , Su Ming-Jang , Yeh Chih-Ching 
Published: September 2023

Abstract
Background
Hepatitis B (HBV) and hepatitis C (HCV) viruses are diseases of global public health concern and are associated with liver cancer. Recent studies have revealed associations between hepatic viral infections and extrahepatic cancers. This study aimed to explore the associations between hepatitis B and C viruses and cancer at baseline in the Taiwan Biobank database while controlling for a wide range of confounding variables.
Methods
In a cross-sectional study of adults aged > 20 years, we compared the distribution of demographic factors, lifestyle, and comorbidities between viral and nonviral hepatic groups using the chi-square test. Univariate and multivariate logistic regressions were performed to observe the associations between hepatitis B and C viral infections and cancers by estimating the odds ratio (OR) and 95% confidence interval (CI). Multivariate regression analysis was adjusted for sociodemographic factors, lifestyle, and comorbidities.
Results
From the database, 2,955 participants were identified as having HCV infection, 15,305 as having HBV infection, and 140,108 as the nonviral group. HBV infection was associated with an increased likelihood of liver cancer (adjusted OR (aOR) = 7.09, 95% CI = 3.48–14.44, P < 0.001) and ovarian cancer (aOR = 3.86, 95% CI = 1.68–8.88, P = 0.002). HCV infection was observed to increase the likelihood of liver cancer (aOR = 4.69, 95% CI = 1.34–16.36, P = 0.015), ovarian cancer (aOR = 5.19, 95% CI = 1.17–22.97, P = 0.030), and kidney cancer (aOR = 10.27, 95% CI = 2.06–52.25, P = 0.005).
Conclusion
Our findings suggest that hepatic viral infections are associated with intra- and extrahepatic cancers. However, being cross-sectional, causal inferences cannot be made. A recall-by-genotype study is recommended to further investigate the causality of these associations.
Prevalence and factors associated with work-related musculoskeletal disorder among Central Sterile Supply Technicians in Thailand
Authors: Chanchai Withaya , Nacglud Ratree , Bunnum Keatkongkrai , Promsorn Nureeya , Duangmusik Laddawan , Suwanrat Warunee , Thonnan Kritsana 
Published: September 2023

Abstract
Objectives
This study aimed to examine the prevalence and factors associated with work-related musculoskeletal disorders among central sterile supply technicians in Thailand.
Methods
A cross-sectional study was administered to CSSTs regarding WRMSDs. Technicians were questioned about the presence and severity of disorders throughout the whole body during the sterilization procedure. The survey was sent to the Central Sterilize Supply Department of 33 centers in Nakhon Si Thammarat province, Thailand.
Results
The prevalence of musculoskeletal symptoms in various body regions was assessed among technicians at the time of questioning (86.0%), during the week (64.2%), and over one year (59.2%). Workers who had been central sterile supply technicians for over 30 years were factored into the assessment of musculoskeletal disorders (OR 8.710, 95% CI 1.444–6.287). CSSTs were also significantly associated with more than ten years of employment (OR 7.859, 95% CI 1.299–5.008). Day shifts (OR 19.196, 95% CI 0.123–0.459), heavy load handling (OR 70.089, 95% CI 10.756-153.554), lifting of instruments (OR 2.007, 95% CI 1.401–5.516), transporting equipment or carts (OR 2.986, 95% CI 1.890–7.273), pushing equipment or carts (OR 9.194, 95% CI 1.401–5.516), carrying instruments (OR 5.584, 95% CI 1.126–4.268), and uncomfortable postures (OR 7.652, 95% CI 1.383–7.570) were factors influencing the causality of musculoskeletal disorders.
Conclusions
These findings suggest that it would be beneficial to promote intervention programs for CSSTs based on the participatory ergonomic (PE) approach and coping methods for musculoskeletal illnesses.
Dehydroepiandrosterone Inhibits ADAMTS Expression via an ERK-dependent Mechanism in Chondrocytes
Authors: Huang Kai , Cai Haili , Cheng Lin , Huang Kai , Zheng Chunwei , Xu Changfu , Sun Shun-tao 
Published: September 2023

Abstract
Background
Osteoarthritis (OA) is a joint disease in which cartilage degradation is the hallmark pathological change. In this study, we investigated the anti-osteoarthritic effects of DHEA in rabbit chondrocytes.
Methods and Results
Polymerase chain reaction was performed to evaluate the expression of a disintegrin and metalloproteinase with thrombospondin motifs (ADAMTS)-4, ADAMTS-5, aggrecan and collagen type 2. In addition, ERK1/2 signaling pathway components were analyzed by Western blotting. In IL-1β-induced chondrocytes, the phosphorylation of ERK1/2 was enhanced, and the downstream catabolic genes, including ADAMTS-4 and ADAMTS-5, were upregulated, while the anabolic genes aggrecan and collagen type 2 were downregulated. DHEA administration restored the IL-1β-induced imbalance in anabolic and catabolic gene expression. In addition, the phosphorylation of ERK1/2 was suppressed by DHEA. Then, PD98059 was used to block the ERK1/2 signaling pathway. The protective effect of DHEA was significantly increased when ERK1/2 signaling was inactivated.
Conclusions
DHEA may exert its protective effect by suppressing ADAMTS in an ERK1/2-dependent manner in rabbit chondrocytes.
Amino acids exchange in patients with CKD as obtained via exome sequencing differ radically from those of natural variants 1000 genome and genomAD databases
Authors: Biochemistry Edem Associate Professor of , Fazaludeen Feroz , Saglam Fatih 
Published: September 2023

Abstract
Background: Nonsynonymous mutations in the coding regions of human genes are responsible for phenotypic differences between humans and for their susceptibility to genetic disease.
Methods: We performed Exome sequencing on CKD patients’ genomic DNA and put the focus in understanding the role played by the amino-acid mutation spectrum (PAM) in human chronic kidney disease CKD patients. More specifically, using SIFT algorithm, we generated the amino acids substitution on proteins and compared the PAM matrix derived from CKD patients representing the amino-acid mutational spectrum to non-disease PAM matrices representing spectra of mutual amino-acid mutation frequencies derived from 1000 genome and genomAD database.
Results: We found a strong and positive correlation in term of mutabilities of amino acids distribution in human proteome and the average distribution of amino acid mutability remains higher in genomAD dataset as compared CKD and 1000 genome. The results also show a strong correlation of mutability between the three datasets, the coefficient of correlation being: (rCKD vs 1kg = 0.9225, rCKD vs genomAD = 0.9431 and rgenomAD vs 1kg = 0.9486) as well probabilistic distribution of amino acids in human proteome between the three datasets.
Conclusion: The amino acids mutability index in CKD dataset was statistically different as compared with those in 1000 genome and genomAD datasets. There is strong positive correlation in mutabilities of amino acids distribution in human proteome and the average distribution of mutability is higher in genomAD dataset as compared CKD and 1000 genome. In the CKD dataset, Arginine remains a common product of mutability from four amino acids like: Tryptophan, Histidine, Glycine and Lysine and the spike of Arginine in blood samples should be an element to trace in diagnostic profile of CKD. The data paved a way for clinical use for amino acids exchanges in chronic kidney disease using illumine platform.
The empirical structure of psychopathology is represented in large language models
Authors: Kambeitz Joseph , Schiffman Jason , Kambeitz-Ilankovic Lana , Ettinger Ulrich , Vogeley Kai 
Published: September 2023

Abstract
Background: Clinical assessment and scientific research in psychiatry is largely based on questionnaires that are employed to detect and quantify psychopathology. The development of large language models offers a new perspective for the analysis of the language and terminology that these questionnaires are based on.
Methods: In the present study, we employed state-of-the-art large language models to derive numerical representations (so called ‘text embeddings’) of semantic and sentiment content of items from established questionnaires for the assessment of psychopathology. We compared the pairwise associations between empirical data and text embeddings in order to test if the empirical structure of psychopathology can be reconstructed by large language models.
Results: Across four large-scale data sets (n=1555, n=1099, n=11807, n=39755), we found a range of significant correlations between empirical item-pair associations and associations derived from text embeddings (r=0.18 to r=0.57, all p<0.05). Machine learning models based on semantic or sentiment embeddings predicted empirical item-pair associations with moderate to high accuracy (r=0.33 to r=0.81, all p<0.05). Similarly, empirical clustering of items and the grouping to established subdomain scores could be partly reconstructed by text embeddings.
Conclusion: The present results demonstrate that large language models are able to represent substantial components of the empirical structure of psychopathology. Consequently, the integration of large language models into mental health research holds the potential to unlock numerous promising avenues. These may encompass improving the process of generating novel questionnaires, optimising generalizability and redundancy of existing questionnaires or facilitating the development of novel conceptualizations of mental disorders.
Statin as Repurposed Drug in Ovarian Cancer: A Comprehensive Review
Authors: Ahmad Asmara , Javed Sadia , Kiran Shumaila 
Published: September 2023

Abstract:
With a prevalence rate of 6.6 per 100,000 women, ovarian cancer is the third most lethal gynecological
tumor in the world. Several factors like family history, nulliparity, late menopause, genetic mutation, and an
unhealthy lifestyle contribute to increasing the risk of ovarian cancer development. Novel research studies
suggest that ovarian cancer may be caused by changes in the lipid metabolic profile that trigger inflammatory responses.
Moreover, ovarian cancer patients will eventually experience chemoresistance. Statin, a competitive inhibitor
of HMG-CoA reductase that is a lipid-lowering drug with pleiotropic effects, seems to be the best
choice to deal with this therapeutic issue. The aim of this review is to highlight the pharmacotherapeutic potential
of statins, especially the repurposing of statin drugs for antitumor mechanisms. This review will also provide
a brief summary of the meta-analysis, and case-control observational studies carried out to examine the impact
of statins on risk reduction and survival in ovarian cancer patients. Furthermore, this review will discuss
the nanotechnological approach for improving the drug's bioavailability and safe and targeted delivery with controlled
release of active ingredients, making statins more effective in preventing and treating ovarian cancer.
MicroRNA-125a-3p Modulate Amyloid β-Protein through the MAPK
Pathway in Alzheimer’s Disease
Authors: Zhu Xi-Chen , Zhu Meng-Zhuo , Lu Jing , Yao Qing-Yu , Hu Jia-Wei , Long Wen-Jun , Ruan Sha-Sha , Dai Wen-Zhuo , Li Rong 
Published: September 2023

Background:
MicroRNA (miR)-125a-3p is reported to play an important role in some
central nervous system diseases, such as Alzheimer’s disease (AD). However, a study has not been
conducted on the mechanism of miR-125a-3p in the pathological process of AD.

Methods:
First, we assessed the expression of miR-125a-3p in AD cohort. Subsequently, we altered
the expressions of miR-125a-3p to assess its role in cell viability, cell apoptosis, amyloid-β
(Aβ) metabolism, and synaptic activity. Finally, we identified its potential mechanism underlying
AD pathology.

Results:
This study unveiled the potential function of miR-125a-3p through modulating amyloid precursor protein processing. Additionally, miR-125a-3p influenced cell survival and activated synaptic expression through the modulation of Aβ metabolism in the mitogen-activated protein kinase (MAPK) pathway via fibroblast growth factor receptor 2.

Conclusion:
Our study indicates that targeting miR-125a-3p may be an applicable therapy for AD
in the future. However, more in vitro and in vivo studies with more samples are needed to confirm
these results.
MDMB-FUBINACA Influences Brain Angiogenesis and the Expression of
VEGF, ANG-1, and ANG-2
Authors: AL-Eitan Laith , Alkhawaldeh Mishael 
Published: September 2023

Aim:
This study aims to explore the impact of the synthetic cannabinoid methyl 2-(1-(4-
fluorobenzyl)-1H-indazole-3-carboxamido)-3,3-dimethylbutanoate (MDMB-FUBINACA) on the angiogenesis
process in human brain microvascular endothelial cells.

Background:
Synthetic cannabinoids (SCs) are substances that mimic the natural components found in
the cannabis plant. SCs are considered prohibited substances that have a clear impact on the central
nervous system (CNS).

Objectives:
The purpose of this study is to explore how MDMB-FUBINACA influences angiogenesis in
human brain microvascular endothelial cells and to clarify the pathways related to the cannabinoid receptors.

Methods::
Human brain microvascular endothelial cells (hBMECs) were grown in the medium containing
Dulbecco Modified Eagle Medium (DMEM/F12) using an endothelial cell growth kit. Endothelial
cell viability was evaluated using the MTT test. Migration ability was measured using the Wound healing
test. The angiogenic capability was measured using a Tube Formation assay. Real-time polymerase
chain reaction (RT-PCR) was utilized to explore the mRNA concentrations following MDMBFUBINACA
treatment. ELISA and Western blotting were also employed to measure the protein levels.

Results:
MDMB-FUBINACA greatly increases tube formation, endothelial cell proliferation, and migration.
Pro-angiogenic factors such as angiopoietins 1 and 2 (ANG-1 and 2) and vascular endothelial
growth factor (VEGF) were shown to be increased at both the RNA and protein levels.

Conclusion:
MDMB-FUBINACA induces the progression of the angiogenesis process by inducing the
expression of pro-angiogenic factors. These findings aim toward developing novel treatments for angiogenesis-
related disorders.
Managing Osteoporosis in COPD
Authors: Shen Lilan , Lv Juanqin , Li Jie , Zhou Jing , Wang Xiaomin 
Published: September 2023

Abstract:
Chronic obstructive pulmonary disease (COPD) is a serious respiratory disease with
high morbidity, disability and mortality worldwide. Every year, many people die from the disease
or its comorbidities. Osteoporosis is a common complication of COPD, which can lead to increased
fractures in COPD patients, aggravate the disease, and then bring great pain and burden
to patients. The possible factors leading to osteoporosis in COPD patients include systemic inflammation,
corticosteroid use, vitamin D deficiency, physical inactivity, tobacco exposure, lower
bone mineral density, hypogonadism, hypoxia, and anemia. In clinical practice, the rate of diagnosis
and treatment of osteoporosis in patients with COPD is low. Several studies demonstrated
that treating osteoporosis with bisphosphonates could improve bone density, make breathing easier,
and improve the quality of life of COPD patients. However, no studies have examined the effect
of anti-osteoporosis therapy on fracture prevention in COPD patients. More research is needed
to clarify how to implement holistic medical interventions in COPD patients with osteoporosis.
We recommend that every COPD patient be screened for osteoporosis and treated with standard
medications for primary osteoporosis.