bluuebunny/crossref_metadata_2025_split huggingface dataset:
Dynamic gravitational excitation of structural resonances in the hertz regime using two rotating bars
Authors: Brack Tobias , Zybach Bernhard , Fankhauser Jonas , Balabdaoui Fadoua , Blunier Stefan , Kaufmann Stephan , Palmegiano Francesco , Scheiwiller Donat , Tomasina Jean-Claude , Dual Jürg 
Published: January 2023

Abstract
With the planning of new ambitious gravitational wave (GW) observatories, fully controlled laboratory experiments on dynamic gravitation become more and more important. Such experiments can provide new insights in potential dynamic effects such as gravitational shielding or energy flow and might contribute to bringing light into the mystery still surrounding gravity. Here we present a laboratory-based transmitter-detector experiment using two rotating bars as transmitter and a 42 Hz, high-Q bending beam resonator as detector. Using a highly precise phase control to synchronize the rotating bars, a dynamic gravitational field emerges that excites the bending motion with amplitudes up to 100 nm/s or 370 pm, which is a factor of 500 above the thermal noise. The two-transmitter design enables the investigation of different setup configurations. The detector movement is measured optically, using three commercial interferometers. Acoustical, mechanical, and electrical isolation, a temperature-stable environment, and lock-in detection are central elements of the setup. The moving load response of the detector is numerically calculated based on Newton’s law of gravitation via discrete volume integration, showing excellent agreement between measurement and theory both in amplitude and phase. The near field gravitational energy transfer is 1025 times higher than what is expected from GW analysis.
An mTOR feedback loop mediates the ‘flare’ (‘rebound’) response
to MET tyrosine kinase inhibition
Authors: Altintas Dogus M , Cerqua Marina , de Laurentiis Angela , Trusolino Livio , Boccaccio Carla , Comoglio Paolo M 
Published: January 2023

Abstract
Targeted therapy significantly impairs tumour growth but suffers from limitations, among which the ‘flare’ (‘rebound’) effect. Among cancers driven by tyrosine kinase receptors, those relying on alterations of the MET oncogene benefit from treatment by specific inhibitors. Previously, we reported that discontinuation of MET tyrosine kinase receptor inhibition causes ‘rebound’ activation of the oncogene, with a post-treatment transient hyperphosphorylation phase that culminates into a dramatic increase in cancer cell proliferation. The molecular mechanisms behind the ‘MET burst’ after treatment cessation are unknown but critically important for patients. Here we identify a positive feedback loop mediated by the AKT/mTOR pathway leading to (a) enhanced MET translation by activating p70S6K and 4EBP1 and (b) MET hyper-phosphorylation by inactivation of the tyrosine-phosphatase PTP1B. The latter effect is due to m-TOR-driven PTP1B phosphorylation of the inhibitory residues Ser50 and Ser378. These data provide in vitro evidence for the use of mTOR inhibitors to prevent the ’flare effect’ in MET targeted therapy, with potential applicative ramifications for patient clinical management.
Beta diversity differences between native and alien conifer understorey vegetation in dry grassland: a multiscale methodology approach
Authors: Bakacsy László , Szepesi Ágnes 
Published: January 2023

Abstract
Alien woody species are one of the most succesful invaders in the world, despite of these species are frequently used for afforestations in several regions such as semi-arid lands. Shrubs and trees can form important patches, creating microhabitats in the open area of arid lands. These vegetations have unique species composition and coexistence of species modes. However, effects of solitary conifer species on the understory vegetation are lacking. This study evaluated the effects of the solitary native and invasive conifer species, Juniperus communis and Pinus nigra on the beta diversity of the surrounding vegetations. To determine the impacts of the conifer invasion on species richness and structure in this habitat, a field study was conducted in Pannonic dry sand grassland in the growing season of 2018. The presence of plant and lichen species were recorded in the vegetation around each six selected solitary conifers. 26 m long circular transects, which consist of 520 units of 5 cm × 5 cm contiguous microquadrats were used for beta diversity estimation. Two information theory models were calculated the baseline transect data, in order to describing the fine-scale spatial structure of each understorey community. The results showed significant differences (p ≤ 0.05) between the two types of understory vegetation functions and these parameters. Our result show, the native conifer J. communis creates a more complex, highly organized microhabitats compared to the invasive, P. nigra. Thus, can conclude that native conifer species has positive effect on the beta diversity of understorey vegetation. Based on our study, the removal of the invasive woody species is required in order to sustain diversity of these habitat and reducing the risk of the introduction of other alien species.
Differential expression patterns of long noncoding RNAs in a pleiomorphic diatom and relation to hyposalinity
Authors: Debit Ahmed , Charton Florent , Pierre-Elies Priscillia , Bowler Chris , de Carvalho Helena Cruz 
Published: January 2023

Abstract
Long non-coding (lnc)RNAs have been shown to have central roles in stress responses, cell identity and developmental processes in multicellular organisms as well as in unicellular fungi. Previous works have shown the occurrence of lncRNAs in diatoms, namely in Phaeodactylum tricornutum, many of which being expressed under specific stress conditions. Interestingly, P. tricornutum is the only known diatom that has a demonstrated morphological plasticity, occurring in three distinct morphotypes: fusiform, triradiate and oval. Although the morphotypes are interchangeable, the fusiform is the dominant one while both the triradiate and the oval forms are less common, the latter often being associated with stress conditions such as low salinity and solid culture media, amongst others. Nonetheless, the molecular basis underpinning morphotype identity in P. tricornutum remains elusive. Using twelve previously published transcriptomic datasets originating from the three morphotypes of P. tricornutum, we sought to investigate the expression patterns of lncRNAs (lincRNAs and NATs) in these distinct morphotypes, using pairwise comparisons, in order to explore the putative involvement of these noncoding molecules in morphotype identity. We found that differentially expressed lncRNAs cluster according to morphotype, indicating that lncRNAs are not randomly expressed, but rather seem to provide a specific (noncoding) transcriptomic signature of the morphotype. We also present evidence to suggest that the major differences in DE genes (both noncoding and coding) between the stress related oval morphotype and the most common fusiform morphotype could be due, to a large extent, to the hyposaline culture conditions rather than to the morphotype itself. However, several lncRNAs associated to each one of the three morphotypes were identified, which could have a potential role in morphotype (or cell) identity in P. tricornutum, similar to what has been found in both animals and plant development.
Concurrent degradation of petroleum sludge and simultaneous rhamnolipid biosurfactant production: An aesthetic bioremediation approach
Authors: Patowary Rupshikha , Rajbongshi Bhagyalakshmi , Devi Arundhuti , Goswami Manisha 
Published: January 2023

Abstract
Petroleum sludge is uncontrollably released in oil field and refineries which can lead to deleterious effect on the environment, as it consists of components of emerging concern such as PAHs, BTEX components, heavy metals, asphaltenes etc. In the present study, a novel bacterium Enterobacter cloacae AR-IASST (01), that have the potential to produce biosurfactant (surface tension reduced to 26.4 mN/m) was utilized to degrade petroleum sludge. A degradation of 86.9% was achieved after 5 days of culture incubation and biosurfactant production was also observed during the degradation process of sludge. In the GC-MS analysis, the peaks numbers reduce from 184 to 13 in the treated sample and complete degradation of PAHs present in the sludge was observed. The biosurfactant was characterized to be rhamnolipid in nature. The biosurfactant exhibited attractive emulsification towards several oils and an E24 of 100% was achieved against crude oil. The biosurfactant was found to be stable over a wide range of temperature, and salt concentration, although it showed sensitivity in high acidic condition. Furthermore, it was found that the bacterial treatment also led to the removal of heavy metals (Ni), zinc (Zn), lead (Pb), iron (Fe), chromium (Cr), copper (Cu), present in the sludge sample. Thus, the present study reveals that the novel bacterium is very potential and can be widely used for restoration of petroleum sludge contaminated sites in future.
Knowledge of Obstetric Danger Signs and Associated Factors among Pregnant Women Attending Antenatal Care in Hargeisa Town Health Institutions, Somaliland: A Cross sectional Study
Authors: Ahmed Abdeta Muktar , Nuh Abdulkadir Mohamed , Hassan Hamse Khalif 
Published: January 2023

Abstract
Introduction: - Maternal mortality is inadmissibly high. About two hundred ninety five thousand mothers lost their life due to pregnancy, delivery and their consequences in 2017 worldwide, in which Sub-Saharan Africa only comprised for about two-thirds of the losses. Inadequate knowledge regarding obstetrics danger signs among mothers and families in poor resource countries is among chief contributing factors for death of mothers. Little is known about the current knowledge of Obstetrics danger signs and influencing factors in Hargeisa, Somaliland.
Objective: - To assess the level of knowledge of obstetrics danger signs and associated factors among pregnant women attending ANC in Hargeisa town health institutions, Somaliland.
Methods and Materials: - Facility based cross sectional study design was used to collect data, among 222 pregnant women attending ANC in selected MCHs in Hargeisa town, Somaliland, July 2022. Eight MCHs were selected by simple random sampling. The sample size was proportionally allocated the selected MCHs based on the number previous month ANC attendees. Systematic random sampling was employed to approach study subjects. A pretested structured interviewer administered questionnaire used to collect the data. Data were checked, cleaned, entered and analyzed using SPSS V. 25. Bivariate and multivariate analysis was performed.
Results: - A total of 222 pregnant women were participated in the study, with 100% response rate. Only 25.3% were knowledgeable about obstetrics danger signs. Educational status of the women and spouse, access to media, health educations given, and place of birth were significantly associated with knowledge of obstetrics danger signs among pregnant women attending ANC in health institutions in Hargeisa. Vaginal bleeding was most frequently mentioned obstetrics danger sign.
Conclusion: - Large proportion of mothers were not knowledgeable about obstetrics danger signs. These mothers are more likely to delay in deciding to seek care. Increasing knowledge or awareness of key obstetrics danger signs need to be given focus as it makes women and their families ready for prompt and appropriate decisions and measures if obstetrics dangers occur.
Plasma Torch Height Tracking Based on Rolling Grey Prediction
Authors: Kye Yong Sik , Choe Yun-Sik , Song Sung Chol , Paek MYong Guk , Ju Gun Sik 
Published: January 2023

Abstract
Among the parameters influencing plasma arc cutting, arc current, cutting speed, and plasma torch height act as the main factors. Especially to maintaining the plasma torch height during plasma cutting process is important. Plasma torch height control system consists of a plasma arc sensing, controller, prediction and stepping motor control system of plasma torch height. To improve the accuracy of plasma torch height control, a height control algorithm based on rolling grey prediction model was proposed and the effectiveness of the proposed algorithm and method was verified through computer simulation and experiments.
Comparing sweet potato chloroplast genome and mitochondrial genome reveals organelle gene transfer
Authors: li Guoliang , zhang hong , lin zhaomiao , li huawei , xu guochun , xu yongqing , ji rongchang , luo wenbin , qiu yongxiang , qiu sixin , tang hao 
Published: January 2023

Abstract
The increasing number of plant mitochondrial genomes (mtDNA) sequenced reveals the extent of transfer from both chloroplast (cpDNA) and nuclear genomes (nDNA). This study created a library and assembled the chloroplast and mitochondrial genomes of the leafy sweet potato Fucaishu18 better to understand the extent of mitochondrial and chloroplast gene transfer. The full-length chloroplast genome of Fucaishu18 (OM808940) is 161387 bp, with 132 genes annotated, including 87 protein-coding genes, 8 rRNA genes, and 37 tRNA genes. The mitochondrial genome (OM808941) was 269578 bp in length and contained 70 functional genes, including 41 protein-coding genes, 6 rRNA genes, and 24 tRNA genes. 68 SSR loci were found in the leafy sweet potato organelle genome, including 54 in the chloroplast genome and 14 in the mitochondria genome. In the sweet potato mitochondrial genome, most genes have RNA editing sites, and the conversion ratio from hydrophilic amino acids to hydrophobic amino acids is the highest, reaching 47.12%. Horizontal transfer occurs in the sweet potato organelle genome; 40 mitochondrial genome segments share high homology with 14 chloroplast genome segments, 33 of which may be derived from chloroplast genome horizontal transfer. The phylogenetic analysis of organelle genes revealed that the leafy sweet potato was closely related to the tetraploid wild species Ipomoea tabascana and the wild diploid species Ipomoea trifida.
Electrochemical Sensing Based on Au particle@SiO2@CQDs Nanocomposites
Authors: Li Huiqin , Huang Fan , Wu Lihua , Lei Hui , Ren Lijun , Zhang Hongge , Deng Cui 
Published: January 2023

Abstract
In this study, carbon quantum dots (CQDs) were first synthesized using a hydrothermal method, and then Au@SiO2 core-shell nanomaterials were synthesized using layer-by-layer assembly. CQDs were adsorbed on the surface of Au@SiO2 nanoparticles through self-assembly to form Au@SiO2@CQDs nanocomposite materials. Transmission electron microscopyand X-ray diffraction were used to characterize the size, shape, element composition, and structure of nanocomposites; ultraviolet-visible absorption spectroscopy and fluorescence spectroscopy were used to analyze the optical properties of nanocomposites. The results show that Au@SiO2@CQDs nanomaterials have a core-shell structure with good morphology and exhibit excellent luminescence characteristics. The electrochemical performance of nanocomposites was characterized using electrochemical means, and a hydrogen peroxide sensor was constructed for the sensitive detection of hydrogen peroxide, thus realizing the rapid and sensitive detection of hydrogen peroxide at levels as low as 0.2 mM.
Haemoglobin diagnostic cut-offs for anaemia in Indian women of reproductive age
Authors: Ghosh Santu , Palika Ravindranadh , Dasi Teena , Varshney Rajeev , Prasannanavar Devraj , Gupta Sourav , Chitikineni Annapurna , Banjara Santosh , Pullakhandam Raghu , Thomas Tinku , Sachdev Harshpal , Kurpad Anura , Kulkarni Bharati 
Published: January 2023

Abstract
Background
The persistent high prevalence of anaemia among Indian women of reproductive age (WRA) despite aggressive long-term iron supplementation, could be related to over-diagnosis from an inappropriately high haemoglobin (Hb) diagnostic cut-off. To develop an appropriate cut-off for Indian WRA, we hypothesized that during iron-folic acid (IFA) supplementation to a mixed (anaemic/non-anaemic) WRA population, the positive slope of the Hb-plasma ferritin (PF) response in anaemic women would inflect into a plateau (zero-response) as a non-anaemic status is reached. The 2·5th percentile of the Hb distribution at this inflection point will be the diagnostic Hb cut-off for iron-responsive anaemia.
Method
A hierarchical mixed effects model, with a polynomial mean and variance model to account for intraclass correlation due to repeated measures, was used to estimate the response curve of Hb to PF, or body iron stores (BIS), in anaemic and non-anaemic WRA (without inflammation), who were receiving a 90-day iron-folic acid (IFA) supplementation.
Results
The Hb response curve at low PF values showed a steep increase, which inflected into a plateau at a PF of 10.1 µg/L. The Hb distribution at the inflection was a normal probability distribution, with mean of 12·3 g/dL. The 2·5th percentile value of this distribution, orthe putative diagnostic Hb cut-off for anaemia, was 10·8 g/dL.
Conclusion
The derived Hb cut-off is lower than the current adult values of 12 g/dL and could partly explain the persistently high prevalence of iron deficiency and anaemia.
Activation of AMPK Ameliorates Acute Severe Pancreatitis by Suppressing Pancreatic Acinar Cell Necroptosis in Obese Mice Models
Authors: Yang Zhiyong , Wang Kunlei , Zhao Anbang , Tayier Dilinigeer , Tan Kai , Song Wenjing , Cheng Qian , Li Xinyin , Chen Zhinan , Wei Qifeng , Yuan Yufeng 
Published: January 2023

Abstract
Obese people with acute pancreatitis(AP) have an increased risk of developing severe acute pancreatitis (SAP), which prolongs the length of hospital stay and increases mortality. Thus, elucidation of the mechanisms through which severe acute pancreatitis occurs in obese individuals will provide clues for possible treatment targets. Differences in early events in obese or lean patients with acute pancreatitis have not been conclusively reported. We selected C57BL/6 mice as lean mice models, ob/ob mice or diet induced obese (DIO) mice as obese mice models and then induced experimental AP in mice via injections of caerulein. There were suppressed p-AMPK expressions in the pancreas of obese mice, compared with same age lean C57BL/6 mice, which were further reduced in AP mice models. Obese AP mice were treated using AICAR, a direct AMPK agonist, which prevented pancreatic damage and cell death, suppressed pancreatic enzyme levels in serum, reduced the areas of fat saponification in the peritoneal cavity, prevented injury in other organs and decreased mice mortality rate. Further assays showed that AICAR activates p-AMPK to stabilize pro-caspase-8. Pro-caspase-8 enhances RIPK3 degradation, inhibits pancreatic acinar cell necroptosis and downregulates the release of pancreatic enzymes. Thus, activation of AMPK by AICAR alleviates pancreatic acinar cell necroptosis and converts SAP to mild acute pancreatitis (MAP) in obese mice.
The Study on the effect of Hydrodynamic flow on the photocatalytic performance of Photocatalytic reactors
Authors: Sundar Kaviya Piriyah , Sellapa Kanmani , Venkatesan Mahalakshmi Nainangkuppam 
Published: January 2023

Abstract
The scalability of photocatalytic reactor is the major challenge due to the inability of the light penetration when the laboratory scale reactors are ascended. It is well known that characteristic length of photocatalytic reactor is one of the influential parameters determining the efficiency of light penetration and also affects the hydrodynamic fluid flow behavior inside the reactor. This study visualizes hydrodynamic flow behavior of three differently configured photocatalytic reactors through numerical simulation of the fluid mass transport inside the reactors. The three photocatalytic reactors are 1. Concentric cylindrical glass tube micro-reactor (CGTR), 2. UV-LED strip photocatalytic reactor (STR) and 3. Classical annular reactor (CAR) from our previous work. The simulations of flow behavior confirmed that CGTR exhibited plug flow regime, STR exhibited arbitrary flow (in-between continuously stirred tank reactor and plug flow reactor) and CAR behaved like continuously stirred tank reactor. Also interpretations of numerical modelling were validated through plotting experimental residence time distribution curve. Further, the comparison of performance parameters revealed that the highest modified Space Time Yield (STYmodified) 225 s− 1and highest modified Photocatalytic Space Time Yield (PSTYmodified) 9.375 s− 1/kW/m3 was obtained for plug flow reactor when compared to continuously stirred tank reactor (CAR) and arbitrary flow reactor (STR). The study confirms that decrease in characteristic length of photocatalytic reactor onsets plug flow regime, which has highest photocatalytic performance efficiency.
Heimdall, an alternative protein issued from a ncRNA related to kappa light chain variable region of immunoglobulins from astrocytes
: a new player in neural proteome
Authors: Capuz Alice , Osien Sylvain , Tristan Cardon , karnoub Melodie , Aboulouard Soulaimane , Romero Antonella Raffo , Duhamel Marie , Cizkova Dasa , Trerotola Marco , Devos David , Kobaissy Firas , Abeele Fabien Vanden , Bonnefond Amelie , Fournier Isabelle , Rodet Franck , Salzet Michel 
Published: January 2023

Abstract
The dogma “One gene, one protein” is clearly obsolete since cells use alternative splicing and generate multiple transcripts which are translated into protein isoforms, but also use alternative translation initiation sites and termination sites on a given transcripts. Alternative open reading frames for individual transcripts give proteins (the alternative Proteins: AltProts) originate from the 5'- and 3'- UTR mRNA regions, frameshifts of mRNA ORFs or from non-coding RNAs. To gain insight into the role of these newly identified alternative proteins in the regulation of cellular functions, it is crucial to assess their dynamic modulation within a framework of altered physiological modifications such as experimental spinal cord injury (SCI). Here, we carried out a longitudinal proteomic study on rat SCI from 12h to 10 days. Based on AltProt predictions, it was possible to identify a plethora of newly predicted protein hits. Among these proteins, some presented a special interest due to high homology with variable chain regions of immunoglobulins. We focus our interest on the one related to Kappa variable light chains which is similarly highly produced by B-cells in the Bence jones disease, but here expressed in astrocytes. This protein, name Heimdall is an Intrinsically disordered protein which is secreted under inflammatory conditions. Immunoprecipitation experiments showed that the Heimdall interactome contained proteins related to astrocyte fate keepers such as “NOTCH1, EPHA3, IPO13”. However, when Heimdall protein was neutralized utilizing a specific antibody or its gene knocked out by CRISPR-Cas9, sprouting elongations were observed in the corresponding astrocytes. Interestingly, depolarization assays and intracellular calcium measurements in Heimdall KO, established a depolarization effect on astrocyte membranes KO cells impacting the astrocyte phenotype sustained by the decrease of NOTCH2. Taken together, Heimdall is a novel neural key player involved in astrocytes gatekeeper phenotype.