bluuebunny/crossref_metadata_2025_split huggingface dataset:
Engineered Bacteria Labeled with Iridium(III) Photosensitizers for Enhanced Photodynamic Immunotherapy of Solid Tumors
Authors: Lin Wenkai , Liu Yu , Wang Jinhui , Zhao Zhennan , Lu Kai , Meng He , Luoliu Ruiqi , He Xiaojun , Shen Jianliang , Mao Zong‐Wan , Xia Wei 
Published: September 2023

AbstractDespite metal‐based photosensitizers showing great potential in photodynamic therapy for tumor treatment, the application of the photosensitizers is intrinsically limited by their poor cancer‐targeting properties. Herein, we reported a metal‐based photosensitizer‐bacteria hybrid, Ir‐HEcN, via covalent labeling of an iridium(III) photosensitizer to the surface of genetically engineered bacteria. Due to its intrinsic self‐propelled motility and hypoxia tropism, Ir‐HEcN selectively targets and penetrates deeply into tumor tissues. Importantly, Ir‐HEcN is capable of inducing pyroptosis and immunogenic cell death of tumor cells under irradiation, thereby remarkably evoking anti‐tumor innate and adaptive immune responses in vivo and leading to the regression of solid tumors via combinational photodynamic therapy and immunotherapy. To the best of our knowledge, Ir‐HEcN is the first metal complex decorated bacteria for enhanced photodynamic immunotherapy.
Three‐phase bio‐nanocomposite natural‐rubber‐based microfibers reinforced with cellulose nanowhiskers and 45S5 bioglass obtained by solution blow spinning
Authors: Silva Michael J. , Dias Yasmin J. , Zaszczyńska Angelika , Kołbuk Dorota , Kowalczyk Tomasz , Sajkiewicz Paweł Ł. , Yarin Alexander L. 
Published: September 2023

AbstractAiming at biomedical applications, the present work developed a new bio‐nanocomposite fibrous mat based on natural rubber (NR) reinforced with 45S5 bioglass particles (BG) and cellulose nanowhiskers (CNW), which reveals excellent mechanical properties, good biocompatibility and bioactivity properties. Analyses of the specimens were conducted by means of morphological observations (SEM) and thermal analysis (TG/DTG), as well as mechanical tests used to verify the effect of the incorporation of BG particles and CNW on the ultimate properties of these flexible NR‐CWN/BG fibrous membranes. An SEM analysis revealed that all filaments possessed a ribbon‐like morphology, with increasing diameters as the BG concentration increased. This likely results from an increased viscosity of the solution used for fiber blowing. In comparison with neat NR fibrous mats, the ultimate mechanical properties of bio‐nanocomposites were significantly improved due to the presence of CNW and BG particles dispersed in the NR matrix. According to the TG/DTG analysis, the specimens' thermal stability was unaffected by the high BG content, and the thermal profiles were similar, with isoprene chains decomposition of the NR occurring between 350 and 450°C. In‐vitro analysis on fibroblasts confirmed that the bio‐nanocomposite fibrous mats are noncytotoxic. It was found that fibrous mats enhanced cellular growth and hold great promise for tissue engineering applications.
Waggle dance‐related gene expression profiles in the honey bee (Apis mellifera) brain
Authors: Yu Kejun , Zhao Yuanhong , Nie Hongyi , Wu Zun , Geng Long , Huang Jingnan , Zhang Zhaonan , Lü Yang , Su Songkun , Li Zhiguo 
Published: September 2023

AbstractThe honey bee dance communication system is one of the most intriguing animal communication signals. It allows foragers to share information related to food sources with nestmates using the waggle dance, which involves the transmission of location information through orientation and duration. Honey bee transcriptomes are dynamic with different stages, expressing an ensemble of differential genes that give rise to substantial behavior diversity. Here, we have provided a global view of mRNA expression profiles in three different stages of waggle dancers including dancing start (DS), dancing end (DE), and dancing cessation (DC). This study yielded 212 differentially expressed genes (DEGs). Among them, we identified 92 DEGs between DS and DC, 118 DEGs between DE and DC, and 2 DEGs between DS and DE. Further, gene ontology (GO) and KEGG analysis suggested that hormone‐mediated signaling pathways and participation in pathways controlling the circadian rhythm, nicotinate, and nicotinamide metabolism were attributed to upregulated genes in DS. In addition, the D1 dopamine receptor in dopaminergic pathways, the peptide signaling involving apidaecin, and neprilysin‐2 may play important roles in intricate behavioral processes.
Evaluating regulatory scenarios to limit U.S. nationwide exposure to cytotoxic haloacetic acids
Authors: Peterson Eric S. , Raseman William J. , Stanford Benjamin D. , Bruce Gretchen M. , Klintworth Heather , Reckhow David 
Published: September 2023

AbstractThe U.S. Environmental Protection Agency (EPA) is considering a regulatory revision of the Disinfectant and Disinfection Byproduct Rule (DBPR) with a goal of limiting nationwide exposure to DBPs of emerging health concern. The occurrence of four brominated haloacetic acids (HAAs), which are generally more toxic in in vitro assays than the five currently regulated HAAs and are candidates for future regulation, were surveyed in 4924 public water systems under EPA's fourth unregulated contaminant monitoring rule (UCMR4). Using UCMR4 data, this study evaluated the nationwide occurrence of nine HAA species and the potential for two regulatory scenarios (the mass sum of all nine HAA species, HAA9, or just the six brominated HAA species, HAA6Br) to control nationwide exposure to the most toxic HAAs. Neither HAA9 nor HAA6Br approaches were effective for identifying water systems that exhibit high HAA exposure, assessed as additive cytotoxicity, because they are more specific to the HAA species that form at high concentrations rather than the species that are most toxic. However, the effectiveness of HAA6Br is highly sensitive to the relative toxicity of one HAA compound, monobromoacetic acid, which has the highest in vitro toxicity among HAAs but also the lowest occurrence and about which little is known regarding in vivo health risks. In contrast to HAA9, systems with high HAA‐associated additive toxicity tend to share similar treatment and disinfectant characteristics as systems with high HAA6Br concentrations. Systems with high source water bromide and total organic carbon were far more likely to use chloramines as a disinfectant residual compared to other systems, but were no more likely to adopt organic precursor removal technologies (biofiltration, granular activated carbon, and ion exchange) than other systems, on average.
The potential role of mechanotransduction in the management of pediatric calvarial bone flap repair
Authors: Anderson Hanna , Hersh David S. , Khan Yusuf 
Published: September 2023

AbstractPediatric patients suffering traumatic brain injuries may require a decompressive craniectomy to accommodate brain swelling by removing a portion of the skull. Once the brain swelling subsides, the preserved calvarial bone flap is ideally replaced as an autograft during a cranioplasty to restore protection of the brain, as it can reintegrate and grow with the patient during immature skeletal development. However, pediatric patients exhibit a high prevalence of calvarial bone flap resorption post‐cranioplasty, causing functional and cosmetic morbidity. This review examines possible solutions for mitigating pediatric calvarial bone flap resorption by delineating methods of stimulating mechanosensitive cell populations with mechanical forces. Mechanotransduction plays a critical role in three main cell types involved with calvarial bone repair, including mesenchymal stem cells, osteoblasts, and dural cells, through mechanisms that could be exploited to promote osteogenesis. In particular, physiologically relevant mechanical forces, including substrate deformation, external forces, and ultrasound, can be used as tools to stimulate bone repair in both in vitro and in vivo systems. Ultimately, combating pediatric calvarial flap resorption may require a combinatorial approach using both cell therapy and bioengineering strategies.
Novel insight into histological and molecular astrocytoma, IDH‐mutant, Grade 4 by the updated WHO classification of central nervous system tumors
Authors: Chen Wenlin , Guo Siying , Wang Yaning , Shi Yixin , Guo Xiaopeng , Liu Delin , Li Yilin , Wang Yuekun , Xing Hao , Xia Yu , Li Junlin , Wu Jiaming , Liang Tingyu , Wang Hai , Liu Qianshu , Jin Shanmu , Qu Tian , Li Huanzhang , Yang Tianrui , Zhang Kun , Wang Yu , Ma Wenbin 
Published: September 2023

AbstractBackgroundThe latest fifth edition of the World Health Organization (WHO) classification of the central nervous system (CNS) tumors (WHO CNS 5 classification) released in 2021 defined astrocytoma, IDH‐mutant, Grade 4. However, the understanding of this subtype is still limited. We conducted this study to describe the features of astrocytoma, IDH‐mutant, Grade 4 and explored the similarities and differences between histological and molecular subtypes.MethodsPatients who underwent surgery from January 2011 to January 2022, classified as astrocytoma, IDH‐mutant, Grade 4 were included in this study. Clinical, radiological, histopathological, molecular pathological, and survival data were collected for analysis.ResultsAltogether 33 patients with astrocytoma, IDH‐mutant, Grade 4 were selected, including 20 with histological and 13 with molecular WHO Grade 4 astrocytoma. Tumor enhancement, intratumoral‐necrosis like presentation, larger peritumoral edema, and more explicit tumor margins were frequently observed in histological WHO Grade 4 astrocytoma. Additionally, molecular WHO Grade 4 astrocytoma showed a tendency for relatively longer overall survival, while a statistical significance was not reached (47 vs. 25 months, p = 0.22). TP53, CDK6, and PIK3CA alteration was commonly observed, while PIK3R1 (p = 0.033), Notch1 (p = 0.027), and Mycn (p = 0.027) alterations may affect the overall survival of molecular WHO Grade 4 astrocytomas.ConclusionsOur study scrutinized IDH‐mutant, Grade 4 astrocytoma. Therefore, further classification should be considered as the prognosis varied between histological and molecular WHO Grade 4 astrocytomas. Notably, therapies aiming at PIK3R1, Notch 1, and Mycn may be beneficial.
Exploring the Chemical and Biological Potential of Jamun (Syzygium cumini (L.) Skeels) Leaves: A Comprehensive Review
Authors: Kumari Neeraj , Kumar Manoj , Chaudhary Nisha , Zhang Baohong , Radha  , Chandran Deepak , Joshi Shourabh , Singh Daljeet , Dey Abhijit , Rajalingam Sureshkumar , Natarajan Krishnaprabu , Muthukumar Muthamilselvan , Mohankumar Pran , Sheri Vijay , Dhumal Sangram , Lorenzo Jose M. 
Published: September 2023

AbstractLeaves of jamun collected as agro by‐produce during the cultivation of jamun is traditionally used as ayurvedic medicine to treat diabetes, gall bladder stones and other ailments. Most of the beneficial effects of jamun leaves are associated with phytochemicals found in jamun leaves such as gallic acid, tannins, mallic acid, flavonoids, essential oils, jambolin, ellagic acid, jambosine, antimellin and betulinic acid. Jamun possess curative activities like anticancer, antidiabetic, antifertility, anti‐inflammatory, antidiarrheal, antimicrobial, antinociceptive, antioxidant, antiradiation, chemotherapeutic, and gastroprotective. The main goal of this review article is to provide information on the nutritional content, phytochemical composition and health promoting properties of jamun leaves. The review of literature based on the phytochemical composition and health promoting benefits of the jamun leaves, suggests that leaves can be used as potential constituent in the formulation of pharmacological drugs. From the review literature it is found that clinical, in‐vivo, in‐vitro studies are still required to check the health promoting effects of jamun leaves extracts on humans.
Access to High‐Purity 7mG‐cap RNA in Substantial Quantities by a Convenient All‐Chemical Solid‐Phase Method
Authors: Noël Mathieu , Guez Theo , Thillier Yann , Vasseur Jean‐Jacques , Debart Françoise 
Published: September 2023

AbstractGiven the importance of mRNA with 5’‐cap, easy access to RNA substrates with different 7mG caps, of high quality and in large quantities is essential to elucidate the roles of RNA and the regulation of underlying processes. In addition to existing synthetic routes to 5’‐cap RNA based on enzymatic, chemical or chemo‐enzymatic methods, we present here an all‐chemical method for synthetic RNA capping. The novelty of this study lies in the fact that the capping reaction is performed on solid‐support after automated RNA assembly using commercial 2’‐O‐propionyloxymethyl ribonucleoside phosphoramidites, which enable final RNA deprotection under mild conditions while preserving both 7mG‐cap and RNA integrity. The capping reaction is efficiently carried out between a 5’‐phosphoroimidazolide RNA anchored on the support and 7mGDP in DMF in the presence of zinc chloride. Substantial amounts of 7mG‐cap RNA (from 1 to 28 nucleotides in length and of any sequence with or without internal methylations) containing various cap structures (7mGpppA, 7mGpppAm, 7mGpppm6A, 7mGpppm6Am, 7mGpppG, 7mGpppGm) were obtained with high purity after IEX‐HPLC purification. This capping method using solid‐phase chemistry is convenient to perform and provides access to valuable RNA substrates as useful research tools to unravel specific issues regarding cap‐related processes.
Impact of diabetes on long‐term outcomes of bifurcation percutaneous coronary intervention. An analysis from the BIFURCAT registry
Authors: Bruno Francesco , Kang Jeehoon , Elia Edoardo , Han Jung‐Kyu , De Filippo Ovidio , Yang Han‐Mo , Gallone Guglielmo , Park Kyung‐Woo , De Luca Leonardo , Kang Hyun‐Jae , Quadri Giorgio , Gwon Hyeon‐Cheol , Chun Woo Jung , Giannino Giuseppe , Hur Seung‐Ho , Han Seung Hwan , Truffa Alessandra , Bin Song Young , Cortese Bernardo , Choi Ki Hong , Chieffo Alaide , Hong Soon‐Jun , Di Pietro Gianluca , Doh Joon‐Hyung , Wanha Wojciech , Nam Chang‐Wook , Kim Hyo‐Soo , Mattesini Alessio , de De Ferrari Gaetano Maria , Koo Bon‐Kwon , D'Ascenzo Fabrizio 
Published: September 2023

AbstractBackgroundIt is still unclear the impact of diabetes mellitus (DM) in complex coronary lesions treated with percutaneous coronary intervention (PCI) which themselves are at increased incidence of adverse events.MethodsBIFURCAT registry encompassed patients treated with PCI for coronary bifurcation lesion from the COBIS III and the RAIN registry. The primary endpoint was the occurrence of major cardiovascular adverse event (MACE), a composite and mutual exclusive of all‐cause death or myocardial infarction (MI) or target‐lesion revascularization (TLR). A total of 5537 patients were included in the analysis and 1834 (33%) suffered from DM.ResultsAfter a median follow‐up of 21 months, diabetic patients had a higher incidence of MACE (17% vs. 9%, p < 0.001), all‐cause mortality (9% vs. 4%, p < 0.001), TLR (5% vs. 3%, p = 0.001), MI (4% vs. 2%, p < 0.001), and stent thrombosis (ST) (2% vs. 1%, p = 0.007). After multivariate analysis, diabetes remained significantly associated with MACE (hazard ratio [HR]: 1.37; confidence interval [CI]: 1.13–1.65; p = 0.001), all‐cause death (HR: 1.65; 95% CI: 1.24–2.19, p = 0.001), TLR (HR: 1.45; CI: 1.03–2.04; p = 0.031) and ST (HR: 1.73, CI: 1.04–2.88; p = 0.036), but not with MI (HR: 1.34; CI: 0.93–1.92; p = 0.11). Among diabetics, chronic kidney disease (HR: 2.99; CI: 2.21–4.04), baseline left ventricular ejection fraction (HR: 0.98; CI: 0.97–0.99), femoral access (HR: 1.62; CI: 1.23–2.15), left main coronary artery (HR: 1.44; CI: 1.06–1.94), main branch diameter (HR: 0.79; CI: 0.66–0.94) and final kissing balloon (HR: 0.70; CI: 0.52–0.93) were independent predictors of MACE at follow‐up.ConclusionsPatients with DM treated with PCI for coronary bifurcations have a worse prognosis due to higher incidence of MACE, all‐cause mortality, TLR and ST compared to the non‐diabetics.
Coils embolization use for coronary procedures: Basics, indications, and techniques
Authors: Loh Shu Xian , Brilakis Emmanuelle , Gasparini Gabriele , Agostoni Pierfrancesco , Garbo Roberto , Mashayekhi Kambis , Alaswad Khaldoon , Goktiken Omer , Avran Alexandre , Knaapen Paul , Nap Alex , Elguindi Ahmed , Tammam Khalid , Yamane Masahisa , Stone Gregg W. , Egred Mohaned 
Published: September 2023

AbstractThe use of coils is fundamental in interventional cardiology and can be lifesaving in selected settings. Coils are classified by their materials into bare metal, fiber coated, and hydrogel coated, or by the deliverability method into, pushable or detachable coils. Coils are delivered through microcatheters and the choice of coil size is important to ensure compatibility with the inner diameter of the delivery catheter, firstly to be able to deliver and secondly to prevent the coil from being stuck and damaged. Clinically, coils are used in either acute or in elective setting. The most important acute indication is typically the sealing coronary perforation. In the elective settings, coils can be used for the treatment of certain congenital cardiac abnormalities, aneurysms, fistulas or in the treatment of arterial side branch steal syndrome after CABG. Coils must always be delivered under fluoroscopy guidance. There are some associated complications with coils that can be acute or chronic, that nictitates regular followed‐up. There is a need for education, training and regular workshops with hands‐on to build the experience to use coils in situations that are infrequently encountered.
Wire‐based antegrade dissection re‐entry technique for coronary chronic total occlusions percutaneous revascularization: Experience from the ERCTO Registry
Authors: Galassi Alfredo R. , Vadalà Giuseppe , Maniscalco Laura , Gasparini Gabriele , Jo Dens , Bozinovic Nenad Z. , Gorgulu Sevket , Gehrig Thomas , Grancini Luca , Ungi Imre , La Scala Eugenio , Ladwiniec Andrew , Stojkovic Sinisa , La Manna Alessio , Tumscitz Carlo , Elhadad Simon , Werner Gerald S. , Sianos Georgios , Garbo Roberto , Carlino Mauro , Mashayekhi Kambis , di Mario Carlo 
Published: September 2023

AbstractBackgroundThe recent development and widespread adoption of antegrade dissection re‐entry (ADR) techniques have been underlined as one of the antegrade strategies in all worldwide CTO consensus documents. However, historical wire‐based ADR experience has suffered from disappointing long‐term outcomes.AimsCompare technical success, procedural success, and long‐term outcome of patients who underwent wire‐based ADR technique versus antegrade wiring (AW).MethodsOne thousand seven hundred and ten patients, from the prospective European Registry of Chronic Total Occlusions (ERCTO), underwent 1806 CTO procedures between January 2018 and December 2021, at 13 high‐volume ADR centers. Among all 1806 lesions attempted by the antegrade approach, 72% were approached with AW techniques and 28% with wire‐based ADR techniques.ResultsTechnical and procedural success rates were lower in wire‐based ADR than in AW (90.3% vs. 96.4%, p < 0.001; 87.7% vs. 95.4%, p < 0.001, respectively); however, wire‐based ADR was used successfully more often in complex lesions as compared to AW (p = 0.017). Wire‐based ADR was used in most cases (85%) after failure of AW or retrograde procedures. At a mean clinical follow‐up of 21 ± 15 months, major adverse cardiac and cerebrovascular events (MACCEs) did not differ between AW and wire‐based ADR (12% vs. 15.1%, p = 0.106); both AW and wire‐based ADR procedures were associated with significant symptom improvements.ConclusionsAs compared to AW, wire‐based ADR is a reliable and effective strategy successfully used in more complex lesions and often after the failure of other techniques. At long‐term follow‐up, patient's MACCEs and symptoms improvement were similar in both antegrade techniques.
A case of hepatic portal venous gas
Authors: Kataoka Shuma , Watanabe Tomoyuki 
Published: September 2023

Key Clinical MessagePortal venous gas (PVG) is rare. When PVG is seen, mesenteric ischemia should be differentiated. US is accurate in the diagnosis of mesenteric ischemia, but the value depends on various condition. Physicians should be familiar with findings of PVG and mesenteric ischemia to aid in appropriate management.
Vogt–Koyanagi–Harada disease with a unilateral presentation in a patient with marijuana overuse: Role of multimodal imaging in suspected patients
Authors: Hosseini Seyedeh Maryam , Gharouni Ahmad , Motamed Shariati Mehrdad 
Published: September 2023

Key Clinical MessageHarada disease could uniquely present with only unilateral symptoms, as was seen in our patient. However, multimodal imaging including ICG angiography could show bilateral involvement. Considering the immunomodulatory effects of Cannabis, the absence of inflammatory findings and the unusual presentation of the disease, in our case, may have been caused by the use of marijuana.AbstractTo report a patient addicted to marijuana with the diagnosis of Vogt–Koyanagi–Harada (VKH) disease with a unilateral presentation. A 24‐year‐old man presented to us with painless decreased vision in his right eye (RE) and photophobia 3 days ago. No history of significant family or past medical history was documented. Spectral‐domain optical coherence tomography (SD‐OCT) of the RE showed multiple areas of subretinal fluid in the macula. Indocyanine green angiography (ICGA) revealed round hypocyanescent dark dots (HDD) of similar size, evenly distributed in both eyes. With the diagnosis of VKH disease, anti‐inflammatory treatment was started. To our knowledge, this is the first reported case of suspected VKH in a patient with marijuana overuse. Regarding the complex effects of tetrahydrocannabinol (THC), the active ingredient of marijuana, on the vascular and immune systems, reaching a definite conclusion is not possible. This report shows the value of multimodal imaging in patients with unusual presentations.
Unusual presentation of transthyretin (ATTR) cardiac amyloidosis: A case report of pruritus as a possible initial symptom and challenging diagnosis
Authors: Baghaee Massood , Mohammadi Mahsa , Moradi Amir , Ghadirzadeh Erfan , Karimi Hanie , Ghorbani Parastoo , Rajabnia Mohsen , Forootan Mojgan 
Published: September 2023

Key Clinical MessageThis case emphasizes the significance of cardiac amyloidosis as a potential diagnosis in individuals manifesting with lesion‐free pruritus and normal liver tests.AbstractAmyloidosis is a complex disorder in which misfolded proteins accumulate in various organs of the body. Cardiac amyloidosis (CA) can lead to heart failure, cardiac arrhythmia, sudden cardiac death, and deposition of proteins in coronary arteries. Diagnosing CA can be difficult, as the cardiac manifestations of amyloidosis can be similar to more prevalent etiologies. In addition, the accumulation of proteins in soft tissues, including the skin, can cause pruritus. In this paper, we present a 70‐year‐old man with generalized pruritus and no skin lesions, later diagnosed as CA after detecting ascites fluid. This case underscores the importance of considering amyloidosis in patients presenting with nonspecific symptoms, particularly those affecting the skin, and highlights the need for increased awareness of this disease among clinicians.