bluuebunny/crossref_metadata_2025_split huggingface dataset:
Field and laboratory study of trace elements deficiency in sheep in Al-Najaf province
Authors: Hatem Abdulameer Abed , Abdul wahid Nabeel Ahmed 
Published: August 2023

This study was designed to evaluate the trace elements in the blood of sheep suffering from clinical signs of mineral deficiency . Thousand (1000) ewe were examined clinically and 32 were suffering from sever mineral deficiency, while 10 animals clinically healthy selected as control group . Blood samples were taken, 32 samples from the infected group and other 10 samples from the control group . Blood samples were analyzed by atomic absorption spectrophotometer. The results showed high deficiency in trace elements as well as presence of significant differences between the affected and the control groups at p<0.05 .The values of Cu, Fe, Co, Zn and Pb in serum of control group were 0.859 ± 0.091 ppm , 2.038 ±0.016 ppm , 0.519 ± 0.076 ppm , 1.028 ± 0.015 ppm , 0.003 ± 0.0005 ppm respectively and in affected group Cu,Fe,Co,Zn And Pb were 0.209 ± 0.006 ppm, 0.294 ± 0.025 ppm, 0.012 ± 0.001 ppm , 0.190 ± 0.007 ppm, 0.053 ± 0.006 ppm respectively. There was no significant variation in toxic metal Pb between affected and control group.at (p<0.05).
Effects of two dose of pomegranate peels powder on some physiological characteristic and libido in the Iraqi Awassi rams
Authors: Hamad Zeaid imad , Mahdi Atheer salih , Mohammed Ali Hussein 
Published: August 2023

Current research studied the effect of two different dose of pomegranate peels powder 20 mg and 40 mg / week / head / on Iraqi Awassi rams, with age 2.5 years and for the period from 29 -October - 2014 to 10-December – 2014.Nine of Awassi rams divided randomly into three groups, as follows: control group which given tap water, second group given 20 mg/ week / head /, and the third group given 40 mg/ week / head .Blood sample collected for measuring the concentration of hemoglobin, and calculate the total number of red blood cells RBCs, and white blood cells WBCs.The weights of the three experimental groups measured at the start of the experiment and at the end of the experiment by scales used for large animals.The results show a significant increase (p<0.05) found in weight at the sixth week of the experiment. While there is no significant differences in the characteristics of blood, the study measured; (hemoglobin, RBCs number, WBCs number), while the libido tested at the end of the experiment. The second and third groups exceeded significantly in (responding time to the first leap, and the responding time to the first Coitus, the number of leaps and the number coitus within 20 minutes.
Comparative study of serum copper concentration in natural copper deficient and healthy Awassi sheep in Al-Najaf city\Iraq
Authors: Mohammed Ahmed Jassim , Kshash Qassim Haleem 
Published: August 2023

This study was conducted on Awassi sheep in Al-Najaf city during October /2014 till January /2015 to detect the main clinical features and copper concentration associated with natural copper deficiency of sheep .
About one hundred clinically copper deficient sheep (50 male,50 female) character by important clinical copper deficient features as affected sheep group and control sheep group which apparent healthy was included one hundred sheep (50 male,50 female) both group sheep was selected randomly and blood samples were collected from each one to estimate serum copper concentration .
Clinical examination of deficient sheep was showed wool abnormalities 60% as higher percentage ,ill-thriftness and retarding of growth 80%, Ataxia(incoordination gait-posterior paralysis) 54% so the predominant signs is paleness of mucous membrane 100% .
So, heart and respiratory rates were increase in deficient sheep (92.11±0.35,36.8±0.8) than in control group (75.8±0.54,29.6±0.19) respectively ,but temperature rate not changes in both animals groups.
Serum copper concentration was (5.58±0.13)µmol/L in deficient sheep when compared with control sheep (15.61±0.56)µmol/L and the serum copper was higher significantly in male than in female in both groups as(6.482±0.21,5.131±0.12) µmol/L in deficient sheep ,(18.21,±0.78,13.014±0.62) ) µmol/L in control group respectively, and copper concentration in adult examined sheep (1-4) years as (5.66±0.68) lower than yearling lamb(9.8±1.03 in deficient group.
This study was conducted to estimate serum copper concentration in clinically copper deficient sheep and the effect of gender and sex on their concentration in Awassi sheep.
Direct detection of Entamoeba bovis in calves infected by diarrhea by using Polymerase chain reaction technique
Authors: Al-shabbani Amal Hassan 
Published: August 2023

This study carried out to direct molecular investigation of Entamoeba bovis from feces samples of calves which suffering from diarrhea that collected from different fields in Al-Diwanyia city by using polymerase chain reaction technique (PCR). This technique was dependent on used specific primers that amplification of small subunit ribosomal RNA gene in Entamoeba bovis. This primers were designed in this study by using NCBI-Genk data base (FN666248.1) and primer 3 plus for primers design. The PCR results were appeared that cattle infected with Entamoeba bovis in percentage of about (36% ) 18 positive samples out of 50 diarrheic samples. We concluded that Entamoeba bovis is important causes of enteric infection in calf whereas, the polymerase chain reaction technique is very specific and rapid assay.
Immunofluorescence Identification of the Endocrine Cells in the Pancreatic Islets of the Camel (Camelus dromedarius)
Authors: Zghair Fatimah Swadi 
Published: August 2023

The aim of the study and characterize the spread of two types of endocrine cells of the pancreatic alpha and beta with hormones in one hump camel (Camelus dromedaries ) by using immunofluorescence technique and compositions histological study of the pancreas. Conducted the study on the pancreas to twelve of the camels from AL-Dwaynia province slaughter house , six animal for histological study and six other for immunofluercent study . To identify two types of endocrine cells in the pancreas with endocrine hormones. By use specific antisera multiple glucagon peptide-1 and Glucose-dependent insulinotropic peptide. The results showed that the distribution of these types of immune cells in the pancreas difference. Immunoreactive cells (beta) cells, the most numerous and take a central position and surround the pancreatic islets . The most distinct delineation of the islets from the surrounding acini. Glucose-dependent insulinotropic peptide -immunoreactive cells (β-cells) were most numerous followed by Glucagon like peptide-1 (α-) -immunoreactive cells in decreasing order . Alpha cells were mostly observed as clumps in the periphery area. The result concluded from the current study, the difference in the distribution of these cells depends on the type of species when compared to the results of this study with other results.
The synergestic effect of probiotic and Levamisole on antibody titre of Newcastle disease in presence of E.coli as stress
Authors: Khammas Emad J. , AL-Zuhariy Mushtaaq T. B. 
Published: August 2023

This study was conducted to investigate the effects of probiotic (poultry star®), and immunmodulator (levamisole) in feed additive and in drinking water as synergistic or each one alone on immune response post routine vaccination and challenge with Newcastle disease virus and E.coli, also on means lesion score in (air sac, heart and liver). one hundred Ross broiler chicks were used from 1 day to 35 day old, divided randomly to five groups (20 birds/group), diet and drinking water offered ad libitium, experimental groups treated as follows: Group one: received poultry star® as feed additive in dose (2×1011 CFU/kg) from one day till the end of the experiment. Group two: received levamisole (10µg/kg B.w.) in drinking water from one day till the end of the experiment. Group three: received levamisole (10µg/kg B.W.) in drinking water plus poultry star in feed additive in dose (2×1011 CFU) from one day till the end of the experiment. Group four: control positive positive (vaccinated not treated). Group five: Control negative (not vaccinated not treated). All groups except the control (negative) vaccinated with ND vaccine (La Sota strain) orally in drinking water at (5 and 15) days and vaccinated with IBD vaccine (intermediate strain) at 12 days. The challenge was carried out through intranasal inoculation with virulent ND virus (100LD50, 1×106) at day 20, exposed to intratracheal inoculation with E.coli (3×107 CFU/ml) at day 22. The obtained results referred to the synergistic effect of probiotic and levamisole in group three that enhanced the immunity against Newcastle virus, also less means level of air sac lesion, pericarditis and perihepatitis score as compared with single effect in the groups (first and second) and also compared with control negative and positive groups. NDV and E.coli Challenge caused high morbidity and no mortality in all vaccinated group compared with control negative. Therefore, all the additive materials (probiotic, levamisole) protected the chicks from bacterial infection E.coli. But did not protect the chicks from infection with virulent NDV in spite of that reducing the severity of infection by reduced the viral replication.
Effects of hydroalcoholic extract of celery (apium graveolens) seed on blood & biochemical parameters of adult male rats
Authors: Al-Kurdy Masar Jabbar Jary 
Published: August 2023

Targeted research study the effect hydroalcoholic extract of celery (apium graveolens) seed on blood & biochemical parameters of adult male rats. Twenty-four adult male rats, were randomly divided into three equal groups. First group (control) daily drenched with 1 ml of distilled water along the experimental period (30 day), second & third groups (G1& G2) daily drenched with drinking water contain( 50 &100 mg/kg b.w) of hydroalcoholic extract of celery seed, respectively. At the end of experiment, blood samples were obtained from each rate for estimation of hemoglobin concentration ,packed cell volume ,WBC count and RBC count. TG, TC, LDL-c and HDL-c concentrations in blood serum were calculated. The result showed that significant increase (P<0.05) in RBC counts, PCV &Hb concentration in the two treated groups as compared with control group, while the result of WBC count showed non- significant differences (P<0.05) in two treated groups compared with control. The result of (TG, TC & LDL-c) concentrations showed a significant decrease (P<0.05) in two treated groups compared with control group. At the same time, the result of HDL-c concentration showed a significant decrease (P<0.05) in G2 compared with G1 and control groups.
Real-Time PCR for direct detection of Clostridium perfringens from horse with enterocolitis infection
Authors: Alwan Jabbar A. , Al-zaidy Ali A. , Abbas Kadhim H. 
Published: August 2023

Clostridium perfringens is one of the most important causative agents of enterocolitis infection in horses. This bacterium capable to produce potential enterotoxin that cause diarrhea in horses. This study was aimed to molecular identification of Clostridium perfringens from horses infected by diarrhea by using Real-Time PCR technique. The Clostridium perfringens type C was identified by using specific primers for enterotoxin (CPE) gene that designed in this study using NCBI-Genbank data base (Genbank code: GQ225719.1) and primer3 plus for primer design. The results show that prevalence of infection by Clostridium perfringens type C was (40%) of 12 positive samples out of 30 fecal samples. This study demonstrated that the direct Real-Time PCR technique is a highly specific and rapid tool for the detection Clostridium perfringens.
Effect of different flax diet concentrations on body weight gain and some of blood parameters of Awassi sheep
Authors: AL-Khafaji Muneer Wahab  Saeed 
Published: August 2023

The study was conducted on 40 Awassi sheep through the year 2014 from the flock reared in Faculty of agriculture / Kufa University. Five treatments included levels of flax (0.0, 2, 4, 6 and 8) %. Body weight in three ages (live weight at 6month, at 9months and at 12 months) was evaluated using variance analyses. Results showed a significant effect (p≤ 0.05) of flax in live body weight the highest BW6, BW9 and BW12 were in the males group that fed with 8% flax it's about 35.22, 46.06 and 58.22 kg respectively compared with the same level of diet flax in female group it's about 30.81, 37.26 and 47.37 kg respectively. Results showed no significant effect of flax and sex in blood parameters of Awassi sheep but there were theoretical differences. The highest total protein value was in male group that fed on 8% of flax its about 74.50 gm/L while the highest value of total lipids was found in the same group it's about 1.56 g/L. The highest value of urea was in male blood that fed on 6% of flax and the highest value of blood cholesterol was found in male group that fed on 8% of flax, it's about 11.28 and 2.51 respectively.
Amelioration effect of methanolic extract of Cyperus rotundus on type 2 diabetes mellitus, thyroid dysfunction and gall stone induce by dexamethasone in male rabbits
Authors: AL-Saeed Muna H. 
Published: August 2023

The research investigated the possible involvement of type2 diabetes mellitus, thyroid dysfunction and gall stone induce by dexamethasone, and therapeutic effect of Cyperus rotundus as antidiabetic potential. This study was carried out in the animal house of the College of Veterinary Medicine - Basrah University. The rabbit was used as a model to study the effects of type2 diabetes mellitus thyroid dysfunction and gall stone induce by dexamethasone, Twenty-four healthy adult domestic rabbits were divided randomly into four groups. The first group (C) had been regarded as control each rabbit in the control group was drenched 3ml of normal saline daily by using gastric tube for 15 days. The second group (DEX) was drenched (0.35 mg / Kg B.W. single dosage) of dexamethasone tablets which were powdered, dissolved in 3ml of normal saline daily for 15 days. The third group (DEX + Cyperus rotunus) was drenched (0.35 mg / Kg B.W. single dosage) of dexamethasone and Cyperus rotunus 0.5g / Kg B.W. single dosage, dissolved in 3ml of normal saline daily for 15 days. The forth group (Cyperus rotunus) was drenched ( 0.5g / Kg B.W. single dosage) of Cyperus rotunus, dissolved in 3ml of normal saline daily for 15 days.
Cyperus rotundus was administered to -induced hyperglycaemic male rabbit and the alterations in serum concentrations of thyroid hormones, total cholesterol, triglycerides and fasting glucose were studied. Simultaneously changes in lipid peroxidation, reduced glutathione (GSH) content, superoxide dismutase and catalase activities in serum (which are commonly affected in diabetes mellitus), were also investigated. Administration of dexamethasone (0.35mg/kg/day orally for 15 days) caused hyperglycaemia with a parallel increase in serum glucose, total cholesterol, triglycerides and lipid peroxidation with a decrease in serum levels of both the thyroid hormones (triiodothyronine, T3 and thyroxine, T4).
However, Cyperus rotundus administration (0.5 g/kg/day orally for 15 days) along with an equivalent amount of dexamethasone reverted most of these changes, including a marked an increase in the serum levels of both thyroid hormones. The present findings reveal that the test extract ameliorates corticosteroid-induced type 2 diabetes mellitus through an increase in serum thyroid hormone concentrations.
Study Immune and productive treatise for broiler vaccinatedwith infectious bursal disease vaccine (IBD vaccine) after added boron powder to the feed
Authors: Ali B. H. , Hamood M. F. , Ali E. J. 
Published: August 2023

This study was performed to investigate changes in growth performance, blood Pictures, weights of internal organs and immune response to infectious bursal disease(IBD vaccine) of broilers fed different levels of Boron (100mg, 200mg and 300mg/kg) in feed from 1 week to 4weeks. 150 broiler chicks (one day old) were randomly divided into five equal groups (each group contain 30), as following: first group ,(G1) was received 100 mg/kg of Boron, second group (G2) was received 200 mg/ kg of Boron, third group, (G3) was received 300 mg/ kg of Boron and fourth group (G4) was not received Boron in feed Just take IBD vaccine and consider as control positive, (G5) was not received Boron in feed and consider as control negative , four groups only vaccinated with IBD vaccine at 10 and 18 day sold, Growth performance of the chicks (Body weight, weight gain and feed intake )were weekly determined, at the end of experiment weights of internal organs (,kidney, spleen and bursa of fabricius) were determined and Blood samples were collected to measured antibody titers (Ab)titers against IBD vaccine by ELISA test ,total protein and determined blood pictures(RBCs, PCV,WBCS,H/L ratio), The results of this study showed that body weight, weight gain, feed intake, , total protein, blood pictures and Ab titers were highly significant increase (p<0.01) in G2 as compared to control group followed by G1 respectively in all weeks. Whereas the weights of internal organs (kidney, bursa of fabricius and spleen ) were significant increase in G3, G2 and G1 respectively. In conclusion, the results indicated that 100mg/kg.feed,200mg.kg.feed levels of Boron supplemented diet of broiler chicken caused improvement of blood parameters growth performance, there's no inhibition of the immunity response of chickens against IBD vaccine.
Isolation and Identification Vibrio parahaemolyticus Which Causing Black Skin Lesion From Golden Fish
Authors: Al-Darwesh Ali adnan , Al-Zamily Khalid Y. , F. Bushra H. , Abbas Nadheema Abed 
Published: August 2023

The aim of this research was aimed to isolate and diagnose the Vibrio. Parahaemolyticus that cause black skin lesion from golden fish and study the histopathological changes in the skin and fins. Seventy-two samples of gold fish were taken from Ornamental Fish markets in Al- Najaf city, The bacteria was diagnosed through culture on normal and special media then conducted by biochemical tests as well as the examination was conducted by API20. The samples of fins and skin were examined for histopathological examination, Histopathologically indecated the presence of degeneration and necrosis of the muscles fibers as well odema were seen in interstitial tissue, , congestion and hemorrhage were recorded in the same area with the presence of infiltration of inflammatory cells among the skeletal muscle. This study concluded that V. parahaemolyticus are responsible for the severe histopathological changes in all organs of infected fishes
Study The Analgesic And Anti-Inflammatory Activity Of Zingiber Officinale Rhizome Extract Comparison With Ibuprofen In Male Mice
Authors: Chyad Amer Hakeem , Ibrahim Omar Salim , Hameed Ahmmed 
Published: August 2023

The aim of the present study was to explore the probable analgesic and anti-inflammatory activity of ethanolic extract of Zingiber officinale by using Hot plate test , acetic acid – induced writhing and Carrageenan induced inflammation in male mice. Thirty Albino male mice (average weight 20g) were used and randomly divided in this study (six animals were used in each groups test for Zingiber officinale in doses of 100, 200 and 300 mg/kg B.W, Ibuprofen 20mg/kg B.W and control ( gave distilled water).The results were expressed as the Mean ±SEM and the statistical significance of differences between groups was analyzed by two Way Analysis of Variance (ANOVA) . The extract showed a significant inhibition of writhing response and increase in hot plate reaction time and also caused significant decrease P<0.05 in paw oedema that induced by Carrageenan comparable with Ibuprofen that standard drugs used. We can conclude from the outcome of the present work that Zingiber officinale extract exert an excellent analgesic and anti-inflammatory effect in the mice and exhibit peripheral and central analgesic effect and anti-inflammatory activity, which may be attributed to the various phytochemicals present in rhizomes of Zingiber officinale .
The Effect of Piracetam against Acetyl Choline (Ach) In Behavioral Changes with Some Biochemical Parameters in Adult Mice
Authors: Al-Shimmary Bassam , Naqi Mohammad , Al Bayati Hala B. Thanoon , Al-Hasan Baraa A. Hussien 
Published: August 2023

The effects of Piracetam against Acetyl Choline (Ach) in adult mice. The Medium Lethal Dose LD50 of Piracetam was 455.5mg/kg B.W. orally and Ach 7.25 mg/kg B.W i.p, then an animal’s are divided to 4 groups First group serves as control group given normal saline, a second 400 mg /kg B.W. o.p. of Piracetam and third group 5 mg/kg B.W. i.p. Ach. Forth group 400 mg /kg B.W. o.p. of Piracetam and 5 mg/kg B.W. i.p. Ach. The behavioral changes, the Run time, swimming, Time on glass, and biochemical parameters in Ach and Piracetam pretreatment significantly (P<0.05) decreased of Run time and swimming, then increased Time on glass when compared with the control. The open field test was significantly (P<0.05) increased the termer, depression when compared with the control. The liver function tests was significantly (P<0.05) increased the in level of GPT and GOT enzymes in the plasma at forth group.
The antimicrobial activity of alcoholic and aqueous extracts of pomegranate fruit peel on Staphylococcus aureus isolated from hair in Najaf
Authors: Abdulbary Meison 
Published: August 2023

There has been considerable interest in the use of plant materials as an alternative method to control pathogenic microorganisms and many compounds of plant products have been shown to be specifically targeted against antibiotic resistant pathogens. The present study was aimed to evaluate the antimicrobial activity of the alcoholic and aqueous extract of pomegranate peel against Staphylococcus aureus isolated from hair. The plant extracts prepared and The antimicrobial effects of the pomegranate extracts were evaluated using method disk inhibition zone , the results shows that the extracts of pomegranate have a good antibacterial growth activity against Staphylococcus aureus isolated from hair with significant differences (P<0.05) with concentrations of the type extract in which there are more susceptible to 100 mg/ ml( the highest concentration ) and the alcoholic extract.
PCR for direct detection food pathogen Listeria monocytogenes and Enteropathogenic Escherichia coli from milk and milk products in Al-Diwanyia city of Iraq
Authors: T.M Esraa 
Published: August 2023

The current study was carried out to directly detect Listeria monocytogenes as well as enteropathogenic Escherichia coli bacterium from daily consumed milk and milk products (buffalo raw milk, local cheese, and local cream) which were collected randomly from different markets of Al-Qadissiya province. In this study the PCR techniques were employed on the basis of amplifying virulence factors (bfpA and hly genes) in L. monocytogenes and enteropathogenic E. coli respectively. Analysis of the results showed the highest prevalence of L. monocytogenes (14.2%) in local cheese which was followed by 6.2% in buffalo raw milk and less prevalence (3.4%) were found in local cream. Analysis of the results of enteropathogenic E. coli revealed highest prevalence (37.5%) in buffalo raw milk while local cheese and local cream showed the same prevalence (20%). This study successfully developed and used PCR technique that was prompt and sensitive/specific in detecting food born pathogenic L. monocytogenes and enteropathogenic E. coli. This technique could be used as an alternative to the other methods, making it possible to identify the risk for these pathogens on public health.