bluuebunny/crossref_metadata_2025_split huggingface dataset:
The structural behaviour of sustainable concrete that incorporate use of agro-industrial waste and recyclable construction materials
Authors: AHMED MUNEER , Khan Suliman , Aziz Sajid 
Published: January 2023

Abstract
This study emphasizes a sustainable approach on alternative green construction techniques that make use of agro-industrial waste and recyclable construction materials to overcome the greenhouse gas emissions and to protect the consumptions of natural resources. In this research experimental study is carried out to evaluate the structural characteristics of self-compacting concrete (SCC) by utilizing ground coal bottom ash (GCBA) as a cement substitute, coir fibre (CF) as a filler with self-consolidating precast concrete beam (PS-B). To determine the fresh state and hardened state properties, a concrete mix design with compressive strength of 45 MPa based on Eurocode-2 guidelines was carried out with different percentages of GCBA and CF. The outcomes revealed that the workability of SCC mix increases up to 10% addition and decreases between 10% and 20% with the incorporation of GCBA. However, it increased significantly by addition of 10% GCBA and 0.5% CF. In addition, the modulus of elasticity, compressive strength, and split tensile strength of SCC were improved substantially with addition of 10% GCBA and 0.5% CF. Lastly, the flexural capacity against four-point bending test in terms of load-deflection behaviour of PS-B was considerably increased by 62.96% by incorporating 0.5% CF - 10% GCBA- compared to the other shell beams, and observed the least deflection.
Genome-wide evolutionary analysis and the expression patterns of RLK gene family in wheat and other plants
Authors: Yan Jun , Su Peisen , Meng Xianyong , Liu Pingzeng 
Published: January 2023

Abstract
Background: Receptor-like kinases (RLKs) gene family contains huge number of members in plants. They are membrane proteins with an extracellular receptor domain, and participate into abiotic and biotic stress responses.
Results: In this study, we identified RLKs in 15 representative plants genomes including wheat, and classified them into 64 subfamilies by using four kinds of phylogenetic trees and HMM models. Conserved exon-intron structures with conserved exon phase in the kinase domain were found in many RLK subfamilies from Physcomitrella patens to Triticum aestivum. Domain distributions of RLKs were also diagrammed. Collinearity events and tandem gene clusters suggested that polyploidizations and tandem duplication events contributed to the member expansions of T. aestivum RLKs. Global expression pattern analysis of T. aestivum, Aegilops tauschii and Brachypodium distachyon RLKs under abiotic and biotic stresses were performed by using public transcriptome data. We also selected 9 RLKs to validate the prediction of transcriptome by using qRT-PCR under drought treatment and with infection of Fusarium graminearum.
Conclusion: In this study, we performed the identification, classification, evolution, and expression patterns of RLKs in wheat and relative plants. Thus, our results will help researchers study evolutionary history and molecular mechanisms of wheat RLKs.
Evidence for a Martian D"-like layer
Authors: Khan Amir , Huang Dongyang , Durán Cecilia , Sossi Paolo , Giardini Domenico , Murakami Motohiko 
Published: January 2023

Abstract
Seismic recordings made as part of the InSight mission (Banerdt et al., 2020) have shown that Mars’s liquid core is ~27% lighter than pure liquid iron (Irving et al., 2022), implying that it contains considerable quantities of light elements. However, geophysically-constrained core compositions require abundances of the volatile elements H, C, and S that are too high with respect to their cosmochemical availability in the potential building blocks that accreted to form Mars (Khan et al., 2022). Here we show that P-waves diffracted along and traversing the core-mantle boundary of Mars in combination with first principles computations of the thermoelastic properties of liquid Fe-rich alloys (Huang et al., 2022) require the presence of a D’’-like molten silicate layer (Samuel et al., 2021) overlying the liquid core of Mars. To match the elastic properties of the core, this layer must be 145+/-25 km thick, causing a reduction in core radius to 1690+/-50 km and an increase in density to 6.65+/-0.15 g/ccm relative to previous models (Staehler et al., 2021, Duran et al., 2022, Khan et al., 2022, Drilleau et al., 2022, Irving et al., 2022). These new bulk core properties are able to reconcile geophysical- and cosmochemical requirements for the light-element content of the Martian core, which is shown to consist of 86--89 wt% Fe-Ni and 11--14 wt% light elements (S, C, O, and H). The chemical characteristics of such a layer may be revealed by products of Martian magmatism.
Efficacy and safety of tranexamic acid in adolescent scoliosis surgery: a systematic review and meta-analysis
Authors: Chen Keyu , Wang Liang , Gao Qingyang , Masood Umar , Zeng Zhimou , Yang Huiliang , Song Yueming 
Published: January 2023

Abstract
Background
Although tranexamic acid (TXA) has been widely used in orthopedic surgery, the literature has not established the efficacy of TXA in adolescent scoliosis surgery. The purpose of this systematic review and meta-analysis is to gather data from randomized controlled trials (RCTs) and non-RCTs to evaluate the efficacy and safety of tranexamic acid (TXA) versus placebo treatment during or after adolescent scoliosis surgery.
Methods
English and Chinese electronic databases including PubMed, Web of Science, Embase, Cochrane, CNKI, and Wan Fang database were searched to identify the relevant literature up until August 2022. The primary outcomes are interoperative blood loss and total blood loss. The secondary outcomes are the need for transfusion, postoperative Hb level, and the change in Hb level. Stata 17 was used for data analysis and the risk of bias was assessed. PRISMA check list is followed to form this article.
Results
Eight studies (670 patients) reported data on the patients that needed a transfusion after surgery. The overall RR showed that there is a significant difference between the TXA and control group regarding the need for transfusion. (RR = 0.547, 95%CI = 0.308 to 0.972, p = 0.04). Twelve studies (795 participants) were included in the meta-analysis for blood loss during surgery. The results of the meta-analysis suggest that TXA can reduce the blood loss of the patients (SMD= -1.23, 95%CI=-1.79 to -0.68, p = 0.000). Six studies (2027 patients) were included in the meta-analysis for total blood loss. The pooled result shows that the total blood loss of the TXA group was significantly lower than that of the control group (SMD=-0.83, 95%CI= -1.22 to -0.43, p = 0.000). Five studies (419 patients) were included in the meta-analysis for the postoperative Hb level and shows a significant outcome (SMD = 0.45, 95%CI = 0.25 to 0.64, p = 0.000). Three studies (268 patients) were included in the meta-analysis for the postoperative Hb level. There is a significant decrease in the TXA group (SMD= -0.27, 95%CI=-0.52 to -0.02, p = 0.034).
Conclusions
The meta-analysis of the data reveals that TXA usage has a significantly better hemostatic effect than the placebo for adolescent scoliosis surgery. However, the surgical operation situations in different studies vary. Therefore, further research is required on gender, operation time, blood transfusion indicators, and other surgical operation variables.
A novel vari-potential bistable nonlinear energy sink for improved vibration suppression: Numerical and experimental study
Authors: Liao Xin , Chen Lin , Zhou Shihua , Zhang Mingliang 
Published: January 2023

Abstract
A novel vari-potential energy bistable nonlinear energy sink (VBNES) is proposed in this paper. By introducing a pair of tuned oscillators (TOs) to dynamically adjust the potential barrier height of the BNES, the excitation threshold of the strong modulated response (SMR) is reduced and its vibration suppression ability is enhanced, especially under ultra-low and wide-amplitude excitation. Firstly, the dimensionless theoretical models of the VBNES and the fixed-potential BNES (FBNES) are constructed by the Lagrange equation. The actual response trajectories on the potential energy surface and restoring force surface are numerically tracked to verify the benefit of variable potential energy effect on vibration suppression. The dynamical characteristics of the typical target energy transfer (TET) mechanisms of the VBNES and their contributions to energy dissipation are analyzed. Furthermore, the transient responses and energy dissipation rates of the VBNES and FBNES with optimal stiffness under impact excitation are compared. The results indicate that the VBNES has higher impact vibration absorption efficiency and stronger robustness. The influences of system parameters on energy dissipation rate are analyzed. Finally, the experimental and numerical studies under harmonic excitation are carried out. The experimental results verify the correctness of the theoretical model. The complex dynamics under numerical frequency and amplitude sweeps demonstrate that the VBNES has a lower excitation threshold of the SMR and a broadband SMR. Parametric studies show that the optimal stiffness depends on the excitation amplitude.
WeChat as a Platform for Blending Problem/Case-Based Learning and Paper Review Method Among Undergraduate Pediatric Orthopaedics Internship: Feasibility and Effectiveness Study
Authors: Chen Junfei , Gao Bingjun , Wang Kunyao , Lei Yinghan , Zhang Shengling , Jin Shaobin , Yang Weiwei , Zhuang Yan 
Published: January 2023

Abstract
Background:

Pediatric orthopedics is a significant and difficult discipline that is for undergraduate students to master. During the COVID-19 pandemic, we relied on the WeChat platform and integrated the advantages of PBL, CBL and paper review teaching methods to establish a blended new online teaching model and demonstrate its feasibility and effectiveness.

Objective:

This study aims to explore a new blended pedagogical method merged PBL, CBL and Paper Review, which is relied on the WeChat platform and based on real clinical cases and frontier literatures for undergraduate participated in a internship pediatric orthopaedics and to demonstrate its feasibility and effectiveness.

Methods:

We enrolled 22 students participating in the Department of Pediatric Orthopaedics. They adopted the WeChat blending pedagogy mode. Their scores of departmental rotation examination were compared with 23 intern doctors who adopted the traditional teaching method. Moreover, an anonymous questionnaire was administered to evaluate students’ perceptions and experiences.

Results:

The total average score of students adopted WeChat blending pedagogy mode and traditional teaching methods was 47.27 and 44.52. There was no statistically significant between WeChat blending pedagogy mode and traditional teaching methodin the aspect of possessing professional accomplishment, gaining knowledge and promoting interpersonal skills (P= 0.07, P = 0.12 and P = 0.65, respectively). In terms of independent clinical thinking, self-improving capability and improving clinical skills, the score of WeChat blending pedagogy mode was 8.00, 8.00 and 6.00, whereas the traditional teaching methods was 6.70, 6.87 and 7.48.
The response rate to the questionnaire was 100% (22/22). and theoverall satisfaction reached 100%. 64%, 86%, 68%, 64% and 59% of students chose very large or large in the aspect of professional accomplishment, knowledge absorption, independent clinical thinking skills, English reading and literature exploring capacity, as well as interpersonal skills. 15 participants considered that WeChat blending pedagogy mode was less helpful to them in promoting the improvement of clinical skills. 9 students thought the WeChat blending pedagogy mode was time-consuming.

Conclusions:

Our study verified the feasibility and effectiveness of WeChat blending pedagogy mode for undergraduate pediatric orthopaedics internship.

Trial registration：

Retrospectively registered.
Machine learning and single-cell sequencing reveal the potential regulatory factors of mitochondrial autophagy in the progression of gastric cancer
Authors: Wei Chen , Ma Yichao , Wang Fei , Chen Yuji , Liao Yiqun , Zhao Bin , Zhao Qi , Wang Daorong , Tang Dong 
Published: January 2023

Abstract
Background

As an important regulatory mechanism to remove damaged mitochondria and maintain the balance between internal and external cells, mitochondrial autophagy plays a key role in the progression and treatment of cancer[1]. The purpose of this study is to comprehensively analyze the role of mitochondrial autophagy-related genes in the progression of gastric cancer (GC) by RNA sequencing (RNA-seq) and single-cell RNA sequencing (scRNA-seq).

Methods

GSE26942, GSE54129,GSE66229,GSE183904 and other data sets were obtained by GEO databases. Using support vector machine recursive feature elimination (SVM-RVF) algorithm and random forest algorithm, the mitochondrial autophagy related genes related to gastric cancer were obtained, respectively. After that, the model was constructed and the inflammatory factors, immune score and immune cell infiltration were analyzed. Furthermore, according to the scRNA-seq data of 28836 cells from 13 GC samples, 18 cell clusters and 7 cell types were identified by scRNA-seq analysis. The expression level and signal pathway of related genes were verified by cell communication analysis. Finally, the regulatory network of cells was analyzed by SCENIC.

Results

MAP1LC3B, PGAW5, PINK1, TOMM40 and UBC are identified as key genes through machine learning algorithms. CXCL12-CXCR4, LGALS9-CD44, LGALS9-CD45 and MIF (CD74 + CD44) pathways may play an important role in endothelial cells with high score scores of T cells and monocytes in tumor environment. CEBPB, ETS1, GATA2, MATB, SPl1 and XBP1 were identified as candidate TF with specific regulatory expression in the GC cell cluster.

Conclusion

The results of this study will provide implications for the study of the mechanism, diagnosis and treatment of mitochondrial autophagy in GC.
MODILM: Towards Better Complex Diseases Classification Using a Novel Multi-omics Data Integration Learning Model
Authors: Zhong Yating , Peng Yuzhong , Lin Yanmei , Chen Dingjia , Zheng Wen , Chen Yuanyuan , Zhang Hao 
Published: January 2023

Abstract
Background
Complex disease classification is an important part of the complex disease diagnosis and personalized treatment process. It has been shown that the integration of multi-omics data can analyze and classify complex diseases more accurately, because multi-omics data are highly correlated with the onset and progression of various diseases and can provide comprehensive and complementary information about a disease. However, multi-omics data of complex diseases are usually characterized by high imbalance, scale variation, high data heterogeneity and high noise interference, which pose great challenges to multi-omics integration methods.
Results
We propose a novel multi-omics data integration learning model called MODILM, to obtain more important and complementary information for complex disease classification from multiple omics data. Specifically, MODILM first initially constructs a similarity network for each omics data using cosine similarity measure, then learns the sample-specific features and intra-association features of single-omics data from the similarity networks using Graph Attention Networks, then maps them uniformly to a new feature space to further strengthen and extract high-level omics-specific features of the omics data using Multilayer Perceptron networks. MODILM then uses a View Correlation Discovery Network to fuse the high-level omics-specific features extracted from each omics data and further learn cross-omics features in the label space, providing unique class-level distinctiveness to classify complex diseases. We conducted extensive experiments on six benchmark datasets having the miRNA expression data, mRNA and DNA methylation data to demonstrate the superiority of our MODILM. The experimental results show that MODILM outperforms state-of-the-art methods, effectively improving the accuracy of complex disease classification.
Conclusions
Our MODILM provides a more competitive way to extract and integrate important and complementary information from multiple omics data, providing a very promising tool for supporting decision making for clinical diagnosis.
Heuristic Evaluation of adverse drug reaction reporting System
Authors: Meraji Marziyhe , Bameri Haniyeh , Ebnehoseini Zahra , Ramezanghorbani Nahid 
Published: January 2023

Abstract
Background
Adverse drug reactions as an ongoing public health issue, affects various aspects of society. This study aims to conduct a comprehensive study of usability and to evaluate usability of National Adverse Drug Reactions Reporting System.
Methods
This study was descriptive and cross-sectional. The National Adverse Drug Reactions Reporting System was evaluated based on the Pierotti heuristic evaluation checklist by seven experts. Every experts completed a heuristic evaluation checklist. The experts also measured the overall severity of each identified heuristic violation on a scale of 0 to 4, where 0 illustrates no problems and 4 illustrates a usability difficulties. Experts' views were extracted and the severity of each problem were analyzed and determined.
Results
A total of 265 problems were identified in the usability of this system. The severity of the problems that were identified according to the defined criteria were: severe problems 10 (3.9%), minor problems 135 (52.7%), Improvable but not necessary 120 (46.87%), and according to the evaluators major and technical problems were zero. The mean range of difficulty was between 1.03 and 8.56. The principle of “Flexibility and minimalist design " had the highest level of severity of the problem.
Conclusion
It is expected that the national adverse drug reaction reporting System will be reviewed again based on the needs of users, standards and changes included in the design. Also can help system designers to overcome its system usability problems.
Bioinformatic analysis of the effect of SNPs in the pig TERT gene on the structural and functional characteristics of the enzyme to develop new genetic markers of productivity traits
Authors: Peka Mykyta , Balatsky Viktor , Saienko Artem , Tsereniuk Oleksandr 
Published: January 2023

Abstract
Background
Telomerase reverse transcriptase (TERT) is responsible for synthesizing telomeric repeats that protect chromosomes from destruction and prevent their fusion, which is critically important for maintaining the stability of the genome. In some cases, mutations in the TERT gene can lead to a deviation in gene expression, impaired enzyme activity, and, as a result, an abnormal shortening of the telomere length. Based on the TERT gene polymorphism, genetic markers of productivity traits of livestock species related to their health, productive life expectancy, resistance to stress and environmental factors can be developed for use in marker-associated selection (MAS). In this study, we propose a bioinformatics-based approach to evaluate the effect of missense SNPs in the pig TERT gene on the function and structure of the enzyme with the prospect of developing genetic markers.
Results
A comparative analysis of the coding and amino acid sequences of the pig TERT was performed with corresponding sequences of other species. The high level of identity found between these sequences of human and pig TERT (77.7% and 71.8%, respectively) made it possible to establish the distribution of polymorphisms in the pig TERT gene in terms of structural-functional domains of the enzyme. A three-dimensional model of the pig TERT structure was obtained by homological modeling based on the human TERT structure. The potential impact of each of the 23 missense SNPs in the pig TERT gene on telomerase function and stability was assessed using predictive bioinformatics tools based on data on the amino acid sequence and structure of pig TERT.
Conclusions
According to bioinformatics analysis of 23 missense SNPs of the pig TERT gene, a predictive effect of SNPs rs789641834 (TEN domain), rs706045634 (TEN domain), rs325294961 (TRBD domain) and rs705602819 (RTD domain) on the structural and functional parameters of the enzyme were established. These SNPs can be considered potential genetic markers of productivity traits associated with pig health, stress tolerance and farming duration. The possibility of their application in MAS should be evaluated in association studies.
Analysis of the efficacy of high hernia sac ligation in adolescent indirect hernia
Authors: Fan Guoyong , Yao Gan 
Published: January 2023

Abstract
Objective
To evaluate the efficacy of high hernia sac ligation in adolescent indirect hernia.
Methods
The data of adolescent patients underwent high hernia sac ligation in the The First People's Hospital of Foshan,China from January 2012 to December 2019 were analyzed retrospectively, mainly collecting their age, gender, weight, surgical method, the hernia ring diameter, operation time, postoperative recurrence rate and postoperative complications.
Results
A total of 71 patients were enrolled, including 62 males (87.32%) and 9 females (12.68%),all patients aged 13–18 years old (mean14.90 ± 1.57years), weighing 28-92kg (mean 53.08 ± 10.53kg), 70patients underwent laparoscopic surgery except 2 with irreducible hernias converted to laparotomy,1 patients underwent open hernia sac high ligation.the hernia ring diameter: 0.5-3cm (mean 1.39 ± 0.49cm), and the diameter of the hernia ring ≤ 2cm in 69 cases (97.18%),The operative length: 12-105min (average 25.45 ± 13.19min), There were 38(53.5%) right-sided hernias, 32 (45.1%) leftsided hernias, 1 (1.4%) performed simultaneous bilaterally.There were 68 (95.8%)reducible hernia,2(2.8%)irreducible hernias ,1(1.4%)incarcerated hernia,Hospital stay :1–5 days (mean 2.37 ± 1.04 days), Follow-up 24-119months (mean 73.56 ± 28.56months), no recurrence, one case of incision infection occured, A second operation was performed 6 months after the operation,4(5.6%)patients had complaints of intermittent pain around the ligation incision site mostly during exerciseIn .
Conclusion
High hernia sac ligation is feasible for treat indirect hernia with a diameter of the hernia ring ≤ 2cm.
Magnesium chelatase subunit D is not only required for chlorophyll biosynthesis and photosynthesis, but also affecting starch accumulation in Manihot esculenta Crantz
Authors: Yang Xingai , cai Jie , Xue Jingjing , Luo Xiuqin , Zhu Wenli , Xiao Xinhui , Xue Maofu , An Feifei , Li Kaimian , Chen Songbi 
Published: January 2023

Abstract
Background: Magnesium chelatase plays an important role in photosynthesis, but only a few subunits have been functionally characterized in cassava.
Results: Herein, MeChlD was successfully cloned and characterized. MeChlD encodes a magnesium chelatase subunit D, which has ATPase and vWA conservative domains. MeChlD was highly expressed in the leaves. Subcellular localization suggested that MeChlD:GFP was a chloroplast-localized protein. Furthermore, the yeast two-hybrid system and BiFC analysis indicated that MeChlD interacts with MeChlM and MePrxQ, respectively. VIGS-induce silencing of MeChlD resulted in significantly decreased chlorophyll content and reduction the expression of photosynthesis-related nuclear genes. Furthermore, the storage root numbers, fresh weight and the total starch content in cassava storage roots of VIGS-MeChlD plants was significantly reduced.
Conclusion: Taken together, MeChlD located at the chloroplast is not only required for chlorophyll biosynthesis and photosynthesis, but also affecting the starch accumulation in cassava. This study expands our understanding of the biological functions of ChlD proteins.