bluuebunny/crossref_metadata_2025_split huggingface dataset:
Development of a bacterial cellulose-gelatin composite as a suitable scaffold for cardiac tissue engineering
Authors: Salehghamari Mohaddeseh , Mashreghi Mansour , Matin Maryam M. , Neshati Zeinab 
Published: September 2023

Abstract
Purpose
Cardiac tissue engineering is suggested as a promising approach to overcome problems associated with impaired myocardium. We introduced a novel scaffold which supports cardiomyocyte attachment and growth and might be a suitable candidate for cardiac tissue engineering.
Methods
Bacterial cellulose (BC) membranes were produced by Komagataeibacter xylinus and coated or mixed with gelatin to make gelatin-coated BC (BCG) or gelatin-mixed BC (mBCG) scaffolds, respectively. BC based-scaffolds were characterized via SEM, FTIR, XRD, and AFM. Neonatal rat-ventricular cardiomyocytes (nr-vCMCs) were cultured on the scaffolds to check the capability of the composites for cardiomyocyte attachment, growth and expansion.
Results
The average nanofibrils diameter in all scaffolds was suitable (~ 30–40 nm) for nr-vCMCs culture. Pore diameter, surface roughness, stiffness in mBCG were in accordance with cardiomyocytes requirements, so that mBCG could better support attachment, growth and expansion of nr-vCMCs.
Conclusion
According to our results, mBCG scaffold was the most suitable composite for cardiomyocyte culture, regarding its physicochemical and cell characteristics. It is suggested that improvement in mBCG stability and cell attachment features may provide a convenient scaffold for cardiac tissue engineering.
Developing low-cost house floors to control tungiasis in Kenya – a feasibility study
Authors: Elson Lynne , Nyawa Shadrack Mwadai , Matharu Abneel , Fillinger Ulrike 
Published: September 2023

Abstract
Context
: Tungiasis is a neglected tropical skin disease endemic in resource-poor communities. It is caused by the penetration of the female sand flea, Tunga penetrans, into the skin causing immense pain, itching, difficulty walking, sleeping and concentrating on school or work. Infection is associated with living in a house with unsealed earthen house floors.
Methods
This feasibility study used a community-based co-creation approach to develop and test simple, locally appropriate, and affordable flooring solutions to create a sealed, washable floor for the prevention of tungiasis. Locally used techniques were explored and compared in small slab trials. The best floor was pilot trialled in a few households with tungiasis cases to assess its durability and costs, feasibility of installation in existing local houses using local masons and explore community perceptions. Disease outcomes were measured to estimate potential impact.
Results
It was feasible to build the capacity of a community-based organization to conduct research, develop a low-cost floor and conduct a pilot trial. The optimal floor was stabilized local subsoil with cement at a 1:9 ratio, installed as a 5 cm depth slab. A sealed floor was associated with a lower mean infection intensity among infected children than in control households (aIRR 0.53, 95%CI 0.29–0.97) when adjusted for covariates. The cost of the new floor was US$3/m2 compared to $10 for a concrete floor. Beneficiaries reported the floor made their lives much easier, enabled them to keep clean and children to do their schoolwork and eat while sitting on the floor. Challenges encountered indicate future studies would need intensive mentoring of masons to ensure the floor is properly installed and households supervised to ensure the floor is properly cured.
Conclusion
This study provided promising evidence that retrofitting simple cement-stabilised soil floors with locally available materials is a feasible option for tungiasis control and can be implemented through training of community-based organisations. Disease outcome data is promising and suggests that a definitive trial is warranted. Data generated will inform the design of a fully powered randomized trial combined with behaviour change communications.
Trial Registration
ISRCTN 62801024 (retrospective 07.07.2023)
Anxiety and depression symptoms among older Chinese migrants during COVID-19: a network analysis
Authors: Zhao Yuefan , Tang Qian , Zhang Chi , Zhang Rongxiu , Yao Jun 
Published: September 2023

Abstract
Background: With the development of an aging society, the mental health of the elderly has become a subject of public health research. In China, many elderly people leave their hometowns due to family conditions, and there are few research topics on the mental health of these elderly people. Anxiety and depression are common psychological problems in elderly individuals. Therefore, in view of the mental health problems of older migrants, this study investigated the network structure of anxiety and depression symptoms in older migrants in our country and determined the central symptoms and bridge symptoms, which provide key symptoms to ensure the mental health of older migrants in our country and further prevent anxiety and depression problems in older migrants.
Methods: To understand the symptoms of depression and anxiety in older Chinese migrants, 469 older migrants were investigated. Depressive symptoms were measured using the Patient Health Questionnaire (PHQ-9), and anxiety symptoms were measured using the Anxiety Scale in the Hospital Anxiety and Depression Questionnaire (HADS-A). Build networks with network analysis. In network analysis, a Gaussian graph model is used to construct an undirected network with a partial correlation coefficient, in which the nodes connected by edges are connected. Nodes represent symptoms, the thickness of the edges connecting nodes represents tightness, and the closer the node is to the middle, the more critical the node is.
Results: The strongest inverse edge connections in the network were for "Relax" in anxiety and "Motor" in depression, and the strongest edges were concentrated in symptoms on the anxiety scale, including "Restless" and "Intense fear", "Tension" and "Fear," and "Butterflies in the stomach" and "restless." It was also revealed that the bridge symptoms in the network were "Relax" and "Restless" in anxiety and "Guilt" in depressive symptoms. Central symptoms in the network include "Restless", "Relax" and "Fear" in anxiety and "Guilt" in depression.
Conclusions:The anxiety symptoms of "restlessness" and "relax" have a great impact on the mental health network of migrant elders. Future intervention and prevention targets could focus on anxiety symptoms in older migrants.
Diabetic Patients’ Satisfaction of Teleconsultations and Associated Factors during COVID-19 Pandemic at Tikur Anbessa Specialized Hospital, Addis Ababa, Ethiopia
Authors: Shimeles Leilina , Asrat Yidnekachew , Tarekegn Getahun , Yesuf Subah Abderehim , Kebede Meskerem Aleka , Betemariam Tesfamariam Aklilu 
Published: September 2023

Abstract
Background: Coronavirus disease 2019 (COVID-19) crisis increased the utilization of teleconsultation globally to reduce the risk of virus transmission. Patients’ satisfaction is an important determinant to ensure quality of health service. However, such patient-related factors are under-investigated and poorly documented in developing countries such as Ethiopia. Therefore, the objective of the study was to assess diabetic patients’ satisfaction with teleconsultations and associated factors during COVID-19 pandemic at Tikur Anbessa Specialized Hospital, Addis Ababa, Ethiopia, 2021.
Methods and Materials: A facility-based, cross-sectional study design was conducted at Endocrinology Unit of Tikur Anbessa Specialized Hospital, Addis Ababa, Ethiopia from 15th of November to 31st of December 2021. Randomly selected 295 diabetic patients who received phone-based medical consultations during the pandemic were interviewed. Data was collected using a pretested, structured interviewer-administered questionnaire. Data was entered, cleaned and analyzed using SPSS version 26. Descriptive statistics was used to describe the data and logistic regression was used to determine associations between dependent and independent variables. A p value <0.05 was considered significant. Tables, figures and texts were used to present the results.
Results: A total of 280 patients responded to the virtual interview successfully, with a response rate of 94.9%. Overall, 148 (52.9%) were satisfied with teleconsultation service. Being urban residents [AOR=3.68 (95%CI: 1.67, 8.09)], being unemployed [AOR=4.57(95%CI:2.31, 9.05)], having disease duration of >10 years [AOR=3.07(95%CI:1.31,7.21)] and absence of diabetic nephropathy [AOR=2.92 (95%CI:1.22,6.98)] were the factors that affected patient satisfaction positively.
Conclusions: With certain contributing factors, more than half of the diabetic patients were satisfied with the teleconsultations during the COVID-19 pandemic. However, a better understanding of context-specific patients’ reasons for dissatisfaction need to be further explored in the planning and implementation of teleconsultations in Ethiopia.
The relationship between neutrophil-lymphocyte, platelet-lymphocyte ratio, monocyte- lymphocyte ratio and cognitive function in schizophrenia
Authors: Zhou Lina , Ma Xiancang , Wang Wei 
Published: September 2023

Abstract
Schizophrenia is a psychotic disorder with unknown pathogenesis, and accompanied by varying degrees of cognitive deficits. Recent studies have shown that dysregulated immune may play an important role in schizophrenia. The aim of this study was to determine the neutrophil-lymphocyte ratio (NLR), platelet-lymphocyte ratio (PLR), and monocyte-lymphocyte ratio (MLR) in patients with schizophrenia, and explore their correlations with cognitive function. 74 patients with schizophrenia and 57 age- and gender-matched healthy controls were recruited in this study with demographic and clinical information. And blood samples were taken and cognitive function were evaluated by MCCB. All the MCCB tasks in this study were found with worse performance in patients with schizophrenia compared to healthy controls. Compare to healthy controls, patients with schizophrenia showed lower Hb levels, and higher monocytes, PLR, and MLR. Spearman correlation analysis showed Hb levels and PLR were positive and negative correlated with global cognitive function respectively. In summary, this study suggests that cognitive deficits in patients with schizophrenia is associated with dysregulated immunity and poor nutrition, which should be paid more attention in the future.
High prevalence of exon-13 variants in USH2A-related retinal dystrophy in Taiwanese population
Authors: Lin Yu-Wei , Huang Yu-Shu , Lin Chien-Yu , Lin Chao-Wen , Wu Chen-Chi , Yang Chang-Hao , Yang Chung-May , Chen Pei-Lung , Chen Ta-Ching 
Published: September 2023

Abstract

Background
USH2A is a common disease-causing gene leading to Usher syndrome, which is an autosomal recessive disorder characterized by retinitis pigmentosa, and was shown to have geographical and ethnical distribution in previous studies. This study provided a deeper understanding of the detailed clinical features using multimodal imaging, genetic spectrum, and genotype–phenotype correlations of USH2A-associated retinal dystrophy in Taiwan.
Results
In our cohort, the mean age at first visit was 47.66 ± 13.54 years, and the mean age at symptom onset was 31.21 ± 15.24 years. Among the variants identified, 23 (50%) were missense, 10 (22%) were splice sites, 8 (17%) were nonsense, and 5 (11%) were frameshift mutations. The most predominant variant was c.2802T > G, which accounted for 21% of patients, and was located in exon 13. Patients with truncated alleles had significantly earlier symptom onset and poorer disease progression regarding visual acuity, ellipsoid zone line length, and hypofluorescent lesions in the macula than those who had the complete gene. However, the clinical presentation revealed similar progression between patients with and without the c.2802T > G variant. During long-term follow-up, the patients had different ellipsoid zone line progression rates and were almost evenly distributed in the fast, moderate, and slow progression subgroups. Although a younger onset age and a smaller baseline intact macular area was observed in the fast progression subgroup, the results showed no significant difference.
Conclusions
This is the first cohort study to provide detailed genetic and longitudinal clinical analyses of patients with USH2A-related retinitis pigmentosa in Taiwan. The mutated allele frequency in exon 13 was high in Taiwan due to the predominant c.2802T > G variant. Moreover, truncated variants greatly impacted disease progression and determined the length of therapeutic windows. These findings provide insight into the characteristics of candidates for future gene therapies.
Optimal fuzzy control of a two-wheel mobile robot equipped with a control moment gyroscope (CMG) stabilizer: Simulation and experiment
Authors: Roshandel D. , Tavakolpour-Saleh Alireza , Esmaeilpour A. 
Published: September 2023

Abstract
This paper presents the modeling, development, and control of a two-wheel mobile robot equipped with a control moment gyroscope (CMG) stabilizer. The study begins by employing a specific method and selecting alternative generalized coordinates to extract the nonholonomic constraint equations of the robot motion. Subsequently, the dynamic governing equations are obtained using the constrained Lagrangian approach. The balance of the robot is controlled in two states: while stationary and while tracking a certain trajectory. Two distinct control approaches are examined. First, the conventional Feedback Linearization Method is applied, and its performance is illustrated. Next, a Fuzzy control is designed and proposed as an optimal and intelligent controller. The superiority of the fuzzy controller over the conventional method is then demonstrated. The accuracy and reliability of the designed controllers are investigated using MATLAB simulations. The controller's performance is evaluated in three different scenarios: stabilizing the robot when deflected from the vertical position, tracking paths while maintaining balance for two reference trajectories, and developing an experimental bicycle robot equipped with a CMG. In these simulations, the robot balance controller and the robot trajectory tracking controller effectively control the balance and path tracking of the robot. Following the simulations, the bicycle robot equipped with a CMG is developed experimentally. The results obtained from the experimental study are presented and discussed to validate the effectiveness of the proposed controllers. Both simulation and experimental findings confirm the proper performance of the proposed intelligent Fuzzy controller in balancing the robot in stationary states and tracking desired trajectories.
Protocol for a randomised controlled trial of psychoeducation material for psychological distress and serotonin levels in women with breast cancer post mastectomy in Indonesia
Authors: Mustikaningsih Dewi , Prajoko Yan Wisnu , Setyowibowo Hari , Anggorowati Anggorowati 
Published: September 2023

AbstractBackground: The prevalence of postmastectomy patients who experience symptoms of anxiety and depression is 40%. The impact of psychological stress due to mastectomy includes loss of self-confidence, specifically, body image, by 63%, lifestyle changes by 51.8%, feeling useless as much as 58% and feeling less cared for by 75%. Patients who experience postmastectomy stress may experience psychological side effects, including a 24% increased chance of cancer recurrence, a 30% increased risk of all-cause mortality, and a 29% greater risk of developing a particular type of breast cancer. The current study will examine whether our recently created nurse-guided psychoeducational material for Indonesian breast cancer survivors who have had mastectomy reduces psychological discomfort.Methods: In Indonesia's Kariadi Hospital Semarang Centre Java, 84 patients will participate in a randomised controlled experiment. Data will be gathered at the start of the intervention (preassessment), 3 to 5 days later (postassessment -1), and 1 to 2 weeks later (follow-up assessment). The primary outcome is the level of psychological distress and serotonin levels. Secondary outcomes are breast cancer post mastectomy score pain level, body image and fatigue.Discussion: If effective, psychoeducation nurse-guided materials will be tested and put into practice in a variety of contexts for city local care that offers health education/psychoeducation to women with breast cancer following mastectomy (such as in the hospital in West Java, Indonesia).Trial registration: TCTR20230501003. Date: Mei 1st,2023.
A case report of neonatal incontinentia pigmenti complicated by severe cerebrovascular lesions in one of the male monozygotic twins
Authors: Lin Xiao feng , Zhang Wei , Zhou Ping 
Published: September 2023

Abstract
Background
This article firstly reports a case of neonatal incontinentia pigmenti only onset in one of the male monozygotic twins with characteristic skin lesions after birth followed by severe cerebrovascular lesions.
Case presentation
A male, the first one of monozygotic twins, was born with multiple yellow pustules all over the body, repeated new herpes at different sites during the course of the disease, aggravated by fusion, warty crusts, and hyperpigmentation, and biopsy pathology suggested eosinophilic spongiform edema of the skin; peripheral blood eosinophils were significantly elevated; brain magnetic resonance imaging revealed diffuse multiple cystic and lamellar abnormal signal areas in the left frontal and parietal lobes. On day 30, the infant showed neurological symptoms such as poor response and apnea, and emergency cranial computed tomography revealed abnormal changes in the left cerebral hemisphere and bilateral cerebellum. After admission, he was given potassium permanganate bath and topical mupirocin for one month, and the skin abnormalities improved. He was treated with mechanical ventilation and vasoactive drugs for 2 days after the cerebrovascular accident, and died the same day after the parents chose hospice care. No deletion variants or point mutations were detected in subsequent genetic tests, and chromosomal copy number variation tests revealed different degrees of chimeric duplications and deletions in different regions of chromosomes Y and 3. The parents were healthy and his twin brother had normal growth and development with no abnormalities at multiple follow-up visits.
Conclusion
Neonate with incontinentia pigmenti in one of the male monozygotic twins is extremely rare and the genetic diagnosis is challenging. Awareness of the combined cerebrovascular lesions needs to be enhanced and potential prevention and treatment methods need to be explored to improve the prognosis.
The Sequelae of Hematopoietic Stem Cell Transplantation in Adolescent and Young Adults: Protocol for A Systematic Review
Authors: Baclig Nikita V , Osuna-Garcia Antonia , Chotai Vivek , Ganz Patricia A. , Brauer Eden R. 
Published: September 2023

Abstract

Background
Hematopoietic stem cell transplantation (HSCT) is a life-saving treatment for Adolescent and Young Adults (age 15–39) with hematologic malignancy. Given the significant developmental milestones usually achieved during this unique life stage, this population is especially vulnerable to the interruption caused by a cancer diagnosis and its treatment. HSCT is a particularly invasive form of cancer therapy with many negative physical, social, and psychological sequelae. The long-term impact of HSCT in Adolescent and Young Adults with hematologic malignancies warrants a systematic investigation of its effects to best shape clinical care and health policy.
Methods
This protocol for a systematic review will focus on the long-term physical, psychological, social, spiritual, and health behavior effects experienced by Adolescents and Young Adults who undergo HSCT for hematologic malignancy. We have constructed a specific search strategy that queries these 5 domains, which will be applied to five databases – Embase, Pubmed, Cochrane Trials and Reviews, PsychInfo, and CINAHL – to identify key literature. Two independent reviewers will perform a title/abstract screen followed by full-text screen using standard screening templates to ensure inclusion of outcomes in the post-acute HSCT period. Risk of bias will be assessed using the University of Adelaide Joanna Briggs Institute Collaboration Critical Appraisal Tools. Data from included studies will be abstracted on study characteristics, study setting, sample characteristics, and outcomes. Given broad scope of the research question, data synthesis will focus on qualitative methods in accordance with Institute of Medicine standards.
Discussion
While Adolescent and Young Adults undergoing Hematopoietic Stem Cell Transplantation for hematologic malignancy are understood to have a unique survivorship experience, the sequelae of this treatment approach in this population have not been previously aggregated. This systematic review intends to expand insight into the Adolescent and Young Adult experiences with HSCT in order to inform age-appropriate survivorship care and deliver this life-saving intervention with the best possible outcomes.
Systematic Review Registration
PROSPERO registration number: CRD42022361663.
Soluble Urokinase Plasminogen Activator Receptor in Vaginally Collected Amniotic Fluid Predicting Fetal Inflammatory Response Syndrome: a prospective cohort study
Authors: Gulbiniene Violeta , Dumalakiene Irena , Balciuniene Greta , Pilypiene Ingrida , Narkeviciute Ieva , Novickij Vitalij , Vysniauskis Gintautas , Ramasauskaite Diana 
Published: September 2023

Abstract
Background. Improving noninvasive antenatal diagnosis of fetal inflammatory response syndrome (FIRS) can assist in the evaluation of prenatal risk and reduce perinatal outcomes. This study aimed to determine whether soluble urokinase-type plasminogen activator receptor (suPAR) in vaginally collected amniotic fluid is significant in identifying FIRS after preterm premature rupture of membranes before 34 weeks of gestation.Study design. This was a prospective cohort study of 114 pregnant women and their newborns after preterm premature rupture of membranes at 22–34+ 6 weeks of gestation. SuPAR was evaluated using an enzyme-linked immunosorbent assay in vaginally collected amniotic fluid. Patients were classified according to the presence or absence of FIRS. FIRS was defined by umbilical cord blood interleukin-6 level > 11 pg/mL or histological funisitis. The data were analyzed using the R package (R–4.0.5).Results. SuPAR was detected in all amniotic fluid samples with a median of 26.23 ng/mL (interquartile range (IQR), 15.19–51.14). The median level of suPAR was higher in the FIRS group than in the non-FIRS group, 32.36 ng/mL (IQR, 17.27–84.16) vs. 20.46 ng/mL (IQR, 11.49–36.63) (P = 0.011), respectively. The presence of histological chorioamnionitis significantly increased the suPAR concentration in the FIRS group (P < 0.001). The areas under the curve for FIRS and for FIRS with histological chorioamnionitis were 0.65 and 0.74, respectively, with an optimum cutoff value of 27.60 ng/mL. Controlling for gestational age, the cutoff of suPAR more than 27.60 ng/mL predicted 3-fold higher odds for FIRS and 6-fold higher odds for FIRS with histologic chorioamnionitis.Conclusion. Soluble urokinase-type plasminogen activator receptor in noninvasively obtained amniotic fluid was an independent predictor for FIRS and concomitant histological chorioamnionitis in patients after preterm premature rupture of membranes before 34 weeks of gestation.