bluuebunny/crossref_metadata_2025_split huggingface dataset:
Screening and identification of genes related to ferroptosis in keratoconus
Authors: Wu Xiaojun , Deng Qing , Han Zhe , Ni Feixue , Sun Daxi , Xu Yuxue 
Published: June 2023

Abstract
Background: Corneal keratoconus (KC) is a dilated corneal disease characterized by a central thinning of the cornea, which protrudes into a conical shape and seriously affects vision. However, due to the complex etiology of keratoconus, it is not yet clear and there is no effective treatment method. Ferroptosis is a novel programmed cell death mechanism related to lipid peroxidation, stress, and amino acid metabolism, playing a crucial role in various diseases. This study aims to explore the relationship between keratoconus and ferroptosis, and provide new insights for the treatment of keratoconus diseases
Methods: The corresponding mRNA microarray expression matrix data of KC patients were obtained from GEO database (GEO204791). Weighted co-expression network analysis (WGCNA) and support vector machine recursive feature elimination (SVM-RFE) were selected to screen hub genes. And the hub genes were overlapped with ferroptosis genes (FRGs) from FerrDb. GO and GSEA were performed to analyze differential pathways, ssGSEA was used to determine immune status, and then, feasible drugs were predicted by gene-drug network. At the same time, we predicted the miRNA and IncRNA of hub genes to identify the underlying mechanism of disease and predicted the treatment of disease.
Results: The epithelial transcriptome from keratoconus tissue mRNA microarray data (GSE204791) was extracted for the main analysis, including eight epithelial cells (EKC) and eight epithelial control cells (EN). The differential genes that were overlapped by WGCAN, SVM-RFE and FRGs were mainly related to oxidative stress, immune regulation, cellular inflammation and metal ion transport. Aldo-keto reductase family 1 member C3 (AKR1C3) was selected out, through further analysis, and negatively correlated with mature CD56 natural killer (NK) cells and macrophages. And then, gene-drug interaction network analysis and miRNA prediction were performed through the website. At the end, A total of four Immune-related drugs (INDOMETHACIN, DAUNORUBICIN, DOXORUBICIN，DOCETAXEL) and a miRNA (has-miR-184) were screened to predict potential drugs and targets for disease treatment.
Conclusion: For the first time , we associated KC with ferroptosis, searched for differential genes to predict the drug targets of gene immunotherapy. Our findings provided a viewpoint and insight for the analysis and treatment of KC.
External Quality Assessment of SDC2 Promoter Hypermethylation Detection in Colorectal Cancer by Novel EQA Materials for Reliable Clinical Testing in China
Authors: Zhang Lijing , Wang Duo , Li Ziqiang , Li Jinming 
Published: June 2023

Abstract
Background
Colorectal cancer (CRC) is a significant public health concern, and the early screening and diagnosis of this disease have a substantial impact on patient outcomes. Syndecan 2(SDC2) promoter hypermethylation has emerged as a crucial marker for the early detection of CRC. However, the clinical detection of SDC2 methylation in China lacks appropriate SDC2 external quality assessment (EQA) materials and nationwide EQA to monitor the accuracy of the assay, which is essential for ensuring reliable and consistent results.
Results
A total of 3 monoclonal cell lines with hypermethylation of SDC2 promoter were constructed, and they were used as a novel EQA material to successfully conduct a nationwide EQA of SDC2 methylation detection. In this EQA report, there are 8 false results (3 commercial diagnostic laboratories had one false positive result each and 2 commercial diagnostic laboratories had one false negative each, while 1 commercial diagnostic lab had three false negative results). Of the 140 participating laboratories, 134 (95.71%) obtained acceptable performance scores and 6 (4.29%) required improvement.
Conclusions
The novel EQA materials helps monitor the accuracy of the assay of the clinical detection of SDC2 promoter hypermethylation in CRC in China, ensuring reliable and consistent results. Improving the reproducibility and accuracy of SDC2 methylation detection contributes to early screening and diagnosis of CRC.
Sleep quality and hypnotics use in patients undergoing maintenance hemodialysis: A cross- sectional study
Authors: Ye Wei , Wang Lizhen , Wang Chengjun , Wang Yu , Qiao Zixuan 
Published: June 2023

Abstract
Background
We investigated sleep quality and hypnotics use in patients undergoing maintenance hemodialysis (MHD) and further explored the effect of sleep quality on quality of life and its associated predictors.
Methods
This study included patients enrolled at hemodialysis center of Jinshan Hospital affiliated to Fudan University in 10/2022, who provided us with demographic, clinical, lifestyle information and recent laboratory examinations. Sleep quality was assessed using Pittsburgh Sleep Quality Index (PSQI). Depression/Anxiety were assessed using Beck Depression/Anxiety Inventory (BDI/BAI). Quality of life was assessed using 36-item Short Form Health Survey (SF-36).
Results
This study included 115 MHD patients with an average value of PSQI 10.77 ± 4.615.Among them,35(30.43%) patients have long-term regular hypnotics use. Hypnotics use was significantly correlated with PSQI(r = 0.646,P = 0.000); PSQI was negatively correlated with the scores of all domains of the SF-36 scale, among which, physical functioning, role-physical, bodily pain, general health and vitality had significantly negative correlation(P < 0.05); Higher PSQI was significantly associated with less residual urine volume(β = 1.127,95% CI:0.083–2.172,P = 0.035), higher BDI score(β = 0.092,95% CI: 0.018–0.167,P = 0.016)and higher systolic pressure (β = 1.992,95% CI:0.698–3.286,P = 0.003).
Conclusions
Poor sleep quality and hypnotics use are very prevalent among patients undergoing MHD. Poor sleep quality significantly impaired their quality of life and may be associated with residual urine volume, depression and systolic pressure.
Knowledge, Attitudes and Practices towards Problem Management Plus among Health Care Professionals in Burera District, Rwanda: A cross – sectional study
Authors: Hakizimana Janvier , Renzaho Jean Nepomuscene , Michael Habtu , Sani Nasiru 
Published: June 2023

Abstract
Introduction: Globally mental health disorders have become the leading cause of disability adjusted life where 183.9% millions of people are affected by mental health disorders and substance use. In Rwanda mental health disorder has increased and been classified among the top 10 causes of YLDs. To cope with it, since 2012 Partners In Health (PIH) in collaboration with Rwandan Ministry of Health implemented mentorship initiative namely MESH at Health Centers level in Burera District. The intervention started with pharmacotherapy and completed with Problem Management Plus in 2017 as all patients did not respond to it. However, there is no study done and published on knowledge, attitude and practice of PM+ since its implementation despite the increasing number of mental health disorders.Methods A cross-sectional study design with mixed approach was conducted in July 2022. The sample was 205 participants (HCPs) from Burera District health facilities. The interviews were conducted and structured questionnaires were filled for quantitative and an interview guide for qualitative approach to collect data before entering and analyzing data into SPSS. Descriptive statistics analysis was used to determine percentages and frequencies while multivariate seconded the bivariate regression analysis to determine the factors associated with PM + practices.Results the majority of respondents 118(60.5%) were females and their age is between 19 and 50, 136 (69.7%). Majority of them have bachelor’s degree 98(50.3%). (57.4%) of HCPs have low level of knowledge on PM + intervention, (48.2%) of them have negative attitudes and (82.1%) of HCPs have poor practices towards PM+. The male respondents were three times more likely to have good practice of PM + intervention compared to females (AOR = 3.277; 95% CI: [1.358–7.665], p = 0.008). The odds of practicing PM + intervention among respondents who did not know it than those who knew it were 1.771 times (AOR = 1.771; 95%CI: [0.534–5.872], p = 0.041). The odds of applying PM + were 12.749 times (AOR = 12.749; 95%CI: 0.751-216.377, p = 0.028) among HCPs who did not know how to use the screening tool compared to those who said that they knew how to use it.Conclusion The knowledge, attitudes and practices among HCPs have great impact on mental health project implementation and training of health care providers on PM + will be crucial.
Pericardial effusion and its relationship to age, sex, causes and degrees
Authors: Alsabbagh Tamara , Tenawi Norman , Shamieh Marwan 
Published: June 2023

Abstract
Introduction
Pericardial effusion is one of the most important and dangerous cardiac manifestations that may lead to death. This study aims to study the characteristics of pericardial effusion in patients attending Damascus Hospital and its relationship to age and gender. Determination of demographic factors associated with pericardial effusion in patients.
Methods
A retrospective study was conducted during the years 2013–2023. The inclusion criteria were all patients with pericardial effusion who were admitted to the cardiovascular department of Damascus Hospital. Chi-square analysis was used to compare infarct type and age groups with sociodemographic factors and patients' medical history variables.
Results
Out of 100 cases of pericardial effusion in the internal cardiac department in Damascus Hospital, 41% of patients were younger than 40 years, compared to 59% who were older than 40 years. Of these, 57% were males, compared to 43% were females. High arterial tension was a prominent history of pericardial effusion in 51% of patients, 12% suffered from heart failure, 19% from diabetes, 20% from kidney failure, and 11% from myocardial infarction. 20% of patients had a mild effusion, 40% a moderate effusion, 43% a heavy effusion, and 10% a pericardial tamponade. Death occurred in only 10% of patients with pericardial effusion, compared to 90% of patients who recovered. Most patients with pericardial effusion had a significant relationship with demographic factors (age, gender), medical history and death.
Conclusion
The results of this study indicate the presence of a combination of risk factors in older adults with pericardial effusion. Programs targeting sociodemographic factors, including smoking, obesity, and comorbidities, are necessary to more effectively address health disparities in pericardial effusion and its negative consequences.
In silico prospection of receptors associated with the biological activity of U1-SCTRX-lg1a: an antimicrobial peptide isolated from the venom of Loxosceles gaucho
Authors: de Oliveira André Souza , Seif Elias Jorge Muniz , Junior Pedro Ismael da Silva 
Published: June 2023

Abstract

Background:The emergence of antibiotic-resistant pathogens generates impairment to human health. U1-SCTRX-lg1a is a peptide isolated from a phospholipase D extracted from the spider venom ofLoxosceles gauchowith antimicrobial activity against Gram-negative bacteria (between 1.15 μM to 4.6 μM). The aim of this study was to suggest potential receptors associated with the antimicrobial activity of U1-SCTRX-lg1a usingin silicobioinformatics tools.Methods:The search for potential targets of U1-SCRTX-lg1a was performed using the PharmMapper server. Molecular docking between U1-SCRTX-lg1a and the receptor was performed using PatchDock software. The prediction of ligand sites for each receptor was conducted using the PDBSum server. Chimera 1.6 software was used to perform molecular dynamics simulations only for the best dock score receptor. In addition, U1-SCRTX-lg1a and native ligand interactions were compared using AutoDock Vina software. Finally, predicted interactions were compared with the ligand site previously described in the literature.Results and discussion:The bioprospecting of U1-SCRTX-lg1a resulted in the identification of forty-nine intracellular proteins originating from Gram-negative microorganisms. Among these, NH3-dependent NAD+synthetase showed the highest dock score. This result suggests that the peptide derived from brown spider venom may interact with residues SER48 and THR160. In addition, the C-terminus has greater affinity for the receptor than the N-terminus.Conclusion:Thein silicobioprospecting of receptors suggests that U1-SCRTX-lg1a may interfere with NAD+production inEscherichia coli, a Gram-negative bacterium, altering the homeostasis of the microorganism and impairing growth.
Identification of circRNA-miRNA-mRNA Network Regulated by Hsp90 in
Human Melanoma A375 Cells
Authors: Fu Qiang , Gao Hengyuan , Liu Kaisheng , Su Juan , Zhang Jianglin , Guo Xiaojing , Yang Fang 
Published: June 2023

Background:
Melanoma is the deadliest form of skin cancer. Heat shock protein
90 (Hsp90) is highly expressed in human melanoma. Hsp90 inhibitors can suppress the growth of
human melanoma A375 cells; however, the underlying mechanism remains unclear.

Methods:
A375 cells were treated with SNX-2112, an Hsp90 inhibitor, for 48 h, and wholetranscriptome
sequencing was performed

Results:
A total of 2,528 differentially expressed genes were identified, including 895 upregulated
and 1,633 downregulated genes. Pathway enrichment analyses of differentially expressed mRNAs
identified the extracellular matrix (ECM)-receptor interaction pathway as the most significantly enriched
pathway. The ECM receptor family mainly comprises integrins (ITGs) and collagens
(COLs), wherein ITGs function as the major cell receptors for COLs. 19 upregulated miRNAs
were found to interact with 6 downregulated ITG genes and 8 upregulated miRNAs were found to
interact with 3 downregulated COL genes. 9 differentially expressed circRNAs in SNX-2112-
treated A375 cells were identified as targets of the ITG- and COL-related miRNAs. Based on the
differentially expressed circRNAs, miRNAs, and mRNAs, ITGs- and COL-based circRNAmiRNA-
mRNA regulatory networks were mapped, revealing a novel regulatory mechanism of
Hsp90-regulated melanoma.

Conclusion:
Targeting the ITG-COL network is a promising approach to the treatment of melanoma.
Zero-valent Iron Nanoparticles: Biogenic Synthesis and their Medical
Applications; Existing Challenges and Future Prospects
Authors: Hayat Minahil , Bukhari Sayyad Ali Raza , Ashraf Muhammad Irfan , Hayat Sumreen 
Published: June 2023

Objective:
In the last decade, nanobiotechnology is emerging as a keen prudence area
owing to its widespread applications in the medical field. In this context, zero-valent iron nanoparticles
(nZVI) have garnered tremendous attention attributed to their cheap, non-toxic, excellent
paramagnetic nature, extremely reactive surface, and dual oxidation state that makes them excellent
antioxidants and free-radical scavengers. Facile biogenic synthesis, in which a biological
source is used as a template for the synthesis of NPs, is presumably dominant among other physical
and chemical synthetic procedures. The purpose of this review is to elucidate plant-mediated
synthesis of nZVI, although they have been successfully fabricated by microbes and other biological
entities (such as starch, chitosan, alginate, cashew nut shell, etc.) as well.

Methods:
The methodology of the study involved keyword searches of electronic databases, including
ScienceDirect, NCBI, and Google Scholar (2008-2023). Search terms of the review included
‘biogenic synthesis of nZVI’, ‘plant-mediated synthesis of nZVI’, ‘medical applications of
nZVI’, and ‘Recent advancements and future prospects of nZVI’.

Results:
Various articles were identified and reviewed for biogenic fabrication of stable nZVI with
the vast majority of studies reporting positive findings. The resultant nanomaterial found great
interest for biomedical purposes such as their use as biocompatible anticancer, antimicrobial, antioxidant,
and albumin binding agents that have not been adequately accessed in previous studies.

Conclusion:
This review shows that there are potential cost savings applications to be made when
using biogenic nZVI for medical purposes. However, the encountering challenges concluded later,
along with the prospects for sustainable future development.
Manufacturing and Applications of Cellulosic Films in Packaging:
An Alternative for Plastic Films
Authors: Akhtar Saamir , Gupta Arun Kumar , Kumar Himanshu 
Published: June 2023

Background:
Different synthetic materials are constantly being utilized as raw materials
for packaging, which might have several drawbacks, like plastic, some of which have poor
lifespans. Plastic can get damaged, scratched, or fractured over time, easily take in tastes and fragrances,
and significantly affect the environment and health. Excessive plastic waste may lead to
environmental pollution and related consequences. Plastics that contaminate food have also been
linked to health problems like high blood pressure, heart palpitations, headaches, nausea, diarrhea,
and flushing.

Objective:
The present review entails the hazards associated with the usage of plastic as a packaging
material and highlights the necessity to reduce its use and promote alternatives and sustainable
production. Thus, the present review portrays the need for biopolymers, types of plant polymers,
methods of cellulose extraction and derivatization, the manufacturing process of cellulosic films
and thereby use as film and coatings for food preservation. The current review also summarizes
different quality control tests for effective utilization as an alternative to synthetic polymers.

Conclusion:
Different polymers derived from agricultural products are excellent candidates for
creating packaging materials. Similar to polysaccharides, these polymers, including nanocomposites,
films, composites, etc., are helpful in a wide range of applications. Cellulose, one of the most
widespread polysaccharides in the world, offers a variety of appealing special qualities. It appears
reasonable and must be considered that cellulose can be used effectively as a raw material for packaging.
In this review, we explore how cellulose is used in the packaging industry to make composites,
coating materials, and edible and non-edible films.
The Effect of DNA Concentration on the HRM Performance in Detecting
Jak2 p.V617F Variant in Patients with Myeloproliferative Neoplasms
Authors: Soltani Atefeh , Shariati Aref , Didehdar Mojtaba , Anoushirvani Ali Arash , Tabaeian Seidamir Pasha , Moradabadi Alireza 
Published: June 2023

Introduction:
Janus kinase 2 (JAK2) is an intracellular signaling protein. JAK2 p.
V617F is a common variant in normal karyotype myeloproliferative neoplasms (MPN). Highresolution
melting (HRM) analysis is one of the essential methods for detecting the JAK2 p.V617F
variant. In this study, we have investigated the effect of DNA concentration on detecting the JAK2
p.V617F variant using the HRM method.

Methods:
Genomic DNA was extracted from human blood and diluted ten times in distilled water
from 1 to 0.03; afterward, HRM was conducted for each dilution (triplicate). Using SPSS v.20.0
software, the mean Tm of each dilution was calculated and compared.

Results:
The HRM results revealed the JAK2 wild type and variant to have Tms of 81/64°C and
80/76°C, respectively. At the endpoint of the pre-amplification, the dilutions had different emissions.
The statistical analysis revealed no statistically significant differences in Tm between samples
with varying DNA concentrations (P value > 0.05).

Conclusion:
There have been no significant differences obtained in the analysis of JAK2 p.V617F
point variant in different DNA dilutions, implying that the HRM analysis has no relation to DNA
concentration.
Phytochemical-based Nanoformulations for Drug-resistant Brain Cancer
Authors: Saini Neha , Bhatt Shailendra , Kumar Manish 
Published: June 2023

Background:
Brain tumor is the deadliest to treat with conventional drug therapy as it has
various side effects on patients leading to organ failure.

Objective:
It is difficult to treat brain cancers or deliver drugs to the targeted organ due to the numerous
challenges faced. The current cytotoxic drugs have serious side effects, such as causing extreme damage
to healthy cells, anemia associated with bone marrow suppression, constipation, small intestine infection,
inflammatory responses, immunodeficiency, and multiorgan toxic effects. Low solubility, poor cell penetration,
hepatic disposition, narrow therapeutic index, and rapid uptake by normal tissues are also a few
challenges. To overcome these issues, it is important to choose plant-based drugs in nano-formulations to
inhibit tumor cell growth without harming the normal cells of an individual. The biggest challenge in
treating tumors is multidrug resistance, which can be overcome by choosing combination therapies of
drugs based on phytochemicals and chemotherapeutic agents, which may lead to minimized adverse effects
on patients with brain tumors.

Results:
As the use of nano-technology for targeted delivery enhances the performance of chemotherapeutic
agents, the drugs with poor characteristics can further be encapsulated in nano-carriers and easily
delivered to the poorly accessible areas of the brain.

Conclusion:
Based on the current progression in nanoformulations, so many new therapeutic approaches
are available to provide better therapeutic results. However, there seems to be a multitude of issues that
need to be addressed in order to ensure efficient results in treating cancer and thus lessening the fatality
rate.
Implementation of Lean management Practices in Azerbaijan (on the Example of a Private Company)
Authors: Alaskarova Yelena , Aleskerova Alina , Dr. Daróczi Miklós 
Published: June 2023

Organizations are facing crucial modifications as global market and conditions change day after day. To keep up with fast changing realities, it’s important to apply innovation decisions to the current stage of organizations. Lean management consolidates tools and techniques to revert from traditional way of operating to the new human oriented one. In the carried-out research its outlined, human resources management plays a big role in spreading and supporting lean initiatives. It was suggested that, Lean and Human resources management are compatible and should work together to obtain sustainable success by implementation of an innovation approach such as Lean. Both lean management and human resource management focus on the same goals with different methods in terms of control of businesses. All positivist approaches and practices are included in these approaches and practices, which are developed with a focus on the interests of the community, in order for the process to be concluded positively. The employee-centred management approach of human resources management is crucial to lead and maintain good environment in the teams. For this reason, in the lean management philosophy, it is aware that the corporate structure has responsibilities in terms of training the employees, eliminating their problems and supporting them in struggling with the responsibilities they undertake. This paper is quantitative, online survey has been conducted with employees of a private company in Azerbaijan and results were analysed in statistical analysis software SPSS and relevant suggestions and recommendation were given for reorganization to enable lean friendly environment on a sample of the given company.
Keywords: Contineous improvement, Human resources management, Industry 4.0, Lean Managament, Lean philisophy.