bluuebunny/crossref_metadata_2025_split huggingface dataset:
Confirmation by necropsy of a high prevalence of porcine cysticercosis in a rural district of Madagascar
Authors: Mananjara Diana Edithe Andria , Rakotoarinoro Mihajamanana , Rakotoarison Valisoa C. , Raliniaina Modestine , Razafindraibe Nivohanitra P. , Ravonirina Claudia , Randriamparany Tantely , Rasamoelina-Andriamanivo Harentsoaniaina , Rakotozandrindrainy Raphaël , Cardinale Eric , Lightowlers Marshall W. , Donadeu Meritxell , Mwape Kabemba E. 
Published: July 2023

AbstractNeurocysticercosis is recognized as an important health issue in the Malagasy population. To date, investigations into prevalence of infection with the causative agent, Taenia solium, in the parasite's natural animal intermediate hosts, have relied on serological methods which have been found to be non-specific. We determined the prevalence of porcine cysticercosis among pigs from a contiguous area of the Betafo and Mandoto administrative districts, Vakinankaratra Region, Madagascar. One hundred and four slaughter-weight pigs were examined by detailed necropsy examination including slicing of the heart, tongue, masseter muscles, diaphragm and carcase musculature. Thirty-seven animals (35.6%) were found infected with T. solium, representing one of the highest rates of infection ever reported, worldwide. These findings highlight the importance of T. solium in Madagascar and support the need for increased efforts to prevent the parasite's transmission to reduce its burden on the health of the Malagasy population.
Gender and Disability in US State Temporary Disability Systems 1942–1949
Authors: REMICK ELIZABETH J. 
Published: July 2023

AbstractDuring the 1940s, four US states established a new form of social insurance, Temporary Disability Insurance (TDI), meant to provide wage replacement to breadwinners unable to work due to nonoccupational illness or injury. The first TDI state, Rhode Island, did not initially exclude coverage of pregnancy-related disabilities, threatening the health of the TDI trust fund. Administrators and lawmakers then sought to reduce or eliminate the pregnancy-related disability benefit on the grounds that pregnancy and related conditions were not “real” disabilities. Subsequently, Rhode Island administrators advised lawmakers in California, New Jersey, and New York to exclude pregnancy-related disabilities from coverage. The breadwinner gender ideology animating New Deal social welfare programs intersected with gendered ideas of disability, creating a form of social insurance that excluded or marginalized pregnancy-related disability and further circumscribed women’s social citizenship.
Incremental Lobby Reform: Elite Interests and Governance Policies
Authors: STRICKLAND JAMES 
Published: July 2023

AbstractCommon examples of governance policies include regulations of lobbying, campaign-finance restrictions, and term limitations. Although the public generally favors these good-government reforms, the laws often restrict the autonomy of political elites. The histories of lobby reform in New York, Georgia, and Michigan illustrate how governance policies might be adopted despite elite opposition. In the states, initial reform efforts came about due to agenda-setting events or policy entrepreneurs. Although legislators adopted lobby reforms, they preferred transparency to other lobby reforms given its limited effect on mutualistic relationships. Initial lobby laws required only disclosure and did not restrict legislator–lobbyist interactions much. Only with the advent of additional events and entrepreneurs were the initial laws strengthened to limit interactions. The histories of reform imply that narratives of policy innovation or diffusion may be complicated somewhat by elite interests and that governance policies, once adopted, may have a unique immunity from repeal.
Christian Democracy, Labor, and the Postwar Politics of Old-Age Pension Reform
Authors: OUDE NIJHUIS DENNIE 
Published: July 2023

AbstractChristian-democratic parties not only constituted the most successful political force in much of Western Europe during most of the twentieth century; their attitudes toward solidaristic welfare reform have arguably also been more diverse than have those of most other major political groupings during this period. Whereas existing studies have mostly attributed this variation to electoral or strategic considerations, this article emphasizes the importance of interest group involvement. It analyzes and compares postwar old-age pension reform in three important Christian-democratic-ruled societies, Belgium, Germany, and the Netherlands, and shows how the very different attitudes of the main Christian-democratic parties toward solidaristic welfare reform in these countries related to the strength and unity of the Christian-democratic labor union movements there.
Simple mandates, monetary rules, and trend-inflation
Authors: Deák Szabolcs , Levine Paul , Pham Son T. 
Published: July 2023

AbstractA mandate framework is proposed for delegating monetary policy to an instrument-independent, but goal-dependent central bank that emphasizes simplicity in both the objectives entering the welfare criterion and those in the instrument rule. It consists of: (i) a simple quadratic loss function penalizing deviations from target variables; (ii) a welfare-optimized, Taylor-type log-linear nominal interest-rate rule with targets that match those in the loss function; (iii) a zero-lower-bound (ZLB) constraint on the nominal interest rate imposing a low unconditional probability of ZLB episodes; and (iv) a long-run inflation target. In an estimated New Keynesian model with these features, we find that for a quarterly probability of 5%, an optimal annual inflation target is close to 2%, weights for real variables in the loss function are small compared with inflation except for the real wage growth mandate and the optimized rules mimic a price-level rule.
Hurricane Impacts on Land in the Central and Eastern Caribbean Since 1494 CE From Written Records
Authors: Chenoweth Michael , Howard Ian 
Published: July 2023

AbstractWritten accounts of hurricanes and their impacts in the Caribbean region date back to 1494 CE We report a new compilation of hurricanes in the longest settled regions of the central and eastern Caribbean region (CECR) that is the most complete yet produced and present basic statistics. We assess likely undercounts of hurricanes due to incomplete documentary records in the earliest part of the record by using the most complete reporting from Puerto Rico relative to the remainder of the CECR. We compare our results with other documentary and proxy data from the region and demonstrate using wavelet analysis significant activity related to the El Niño‐Southern Oscillation (ENSO) at inter‐annual time scales and lower frequency activity from decadal to multi‐decadal scales. Our results offer evidence that, along with ENSO, fluctuations in sulfate aerosols are a possible contributor to the hurricane history. The frequency of hurricane landfalls and impacts in the CECR was lower in the 20th century than any time since 1494 CE.
Beyond Simple Trend Tests: Detecting Significant Changes in Design‐Flood Quantiles
Authors: Awasthi C. , Archfield S. A. , Reich B. J. , Sankarasubramanian A. 
Published: July 2023

AbstractChanges in annual maximum flood (AMF), which are usually detected using simple trend tests (e.g., Mann‐Kendall test (MKT)), are expected to change design‐flood estimates. We propose an alternate framework to detect significant changes in design‐flood between two periods and evaluate it for synthetically generated AMF from the Log‐Pearson Type‐3 (LP3) distribution due to changes in moments associated with flood distribution. Synthetic experiments show MKT does not consider changes in all three moments of the LP3 distribution and incorrectly detects changes in design‐flood. We applied the framework on 31 river basins spread across the United States. Statistically significant changes in design‐flood quantiles were observed even without a significant trend in AMF and basins with statistically significant trend did not necessarily exhibit statistically significant changes in design‐flood. We recommend application of the framework for evaluating changes in design‐flood estimates considering changes in all the moments as opposed to simple trend tests.
Thank You to Our 2022 Peer Reviewers
Authors: Manighetti Isabelle , Abercrombie Rachel , Bernabé Yves , Bostock Michael , Dekkers Mark J. , Ide Satoshi , Schmitt Douglas R. , Han Shin‐Chan , Tregoning Paul 
Published: July 2023

AbstractEditors of JGR‐Solid Earth express their appreciation to those who served as peer reviewers for the journal in 2022.
Experiences and recommendations from people with spinal cord injury following participation in a disability education session at an allopathic medical school: a qualitative study
Authors: Pekmezaris Renée , Patel Vidhi , Herman Paige , Stein Adam B. , Bloom Ona 
Published: July 2023

Abstract
Background
Students in half of US medical schools do not receive formal instruction in providing medical care for people with disabilities. To address this gap in training, our medical school developed several strategies, including a session for second year medical students to address communication skills, knowledge, and attitudes relevant to delivering healthcare for people with disabilities. Here, our objective was to explore perceptions of people with spinal cord injury (SCI) who participated in the session on its content and structure.

Methods
Qualitative research using a focus group of people with SCI who participated in an educational session for medical students in an LCME accredited allopathic US medical school. A purposive sample of adults with SCI (N = 8) participated in a focus group. Data were analyzed using a six-phase thematic analysis.

Results
Participants favorably viewed the educational session, felt their participation was valuable, and had suggestions for its improvement. Four major themes were identified: (1) session format, content; (2) addressing student discomfort and avoidance behaviors; (3) increasing student knowledge and preparation; and (4): important lessons from discussions of past and role-played doctor-patient interactions.

Conclusions
First-person input from people with SCI is critical to improve medical education and healthcare provision to the SCI community. To our knowledge, this is the first study to report feedback from stakeholders providing specific recommendations for teaching disabilities awareness to undergraduate medical students. We expect these recommendations to be relevant to the SCI and medical education communities in improving healthcare for people with SCI and other disabilities.
The TLR3 L412F polymorphism prevents TLR3-mediated tumor cell death induction in pediatric sarcomas
Authors: Bisaccia Joseph , Meyer Swann , Bertrand-Chapel Adrien , Hecquet Quentin , Barbet Virginie , Kaniewski Bastien , Léon Sophie , Gadot Nicolas , Rochet Isabelle , Fajnorova Iveta , Leblond Pierre , Cordier-Bussat Martine , Corradini Nadège , Vasiljevic Alexandre , Billaud Marc , Picard Cécile , Broutier Laura , Gallerne Cindy , Dutour Aurélie , Blay Jean-Yves , Castets Marie 
Published: July 2023

AbstractToll-like receptor 3 (TLR3) is a pattern recognition receptor mainly known for its role in innate immune response to infection. Indeed, binding of double-stranded RNA (dsRNA) to TLR3 triggers a pro-inflammatory cascade leading to cytokine release and immune cell activation. Its anti-tumoral potential has emerged progressively, associated with a direct impact on tumor cell death induction and with an indirect action on immune system reactivation. Accordingly, TLR3 agonists are currently being tested in clinical trials for several adult cancers. Meanwhile, TLR3 variants have been linked to auto-immune disorders, and as risk factors of viral infection and cancers. However, aside from neuroblastoma, TLR3 role in childhood cancers has not been evaluated. Here, by integrating public transcriptomic data of pediatric tumors, we unveil that high TLR3 expression is largely associated with a better prognosis in childhood sarcomas. Using osteosarcomas and rhabdomyosarcomas as models, we show that TLR3 efficiently drives tumor cell death in vitro and induces tumor regression in vivo. Interestingly, this anti-tumoral effect was lost in cells expressing the homozygous TLR3 L412F polymorphism, which is enriched in a rhabdomyosarcomas cohort. Thus, our results demonstrate the therapeutic potential associated with the targeting of TLR3 in pediatric sarcomas, but also the need to stratify patients eligible for this clinical approach with respect to the TLR3 variants expressed.
Kinome-wide CRISPR-Cas9 knockout screens revealed PLK1 as a therapeutic target for osteosarcoma
Authors: Wang Renxian , Wang Dingding , Bai Xueshan , Guo Jianxun , Xia Songxia , Cheng Yuning , Gu Yani , Wang Qian , Nie Jingjun , Chen Dafu , Liu Weifeng , Liang Junbo 
Published: July 2023

AbstractOsteosarcoma is the most common malignant bone tumor, tending to be aggressive and recurrent. The therapeutic development for treating osteosarcoma has been largely hampered by the lack of effective and specific targets. Using kinome-wide CRISPR-Cas9 knockout screens, we systematically revealed a cohort of kinases essential for the survival and growth of human osteosarcoma cells, in which Polo-like kinase 1 (PLK1) appeared as a specific prominent hit. PLK1 knockout substantially inhibited proliferation of osteosarcoma cells in vitro and the tumor growth of osteosarcoma xenograft in vivo. Volasertib, a potent experimental PLK1 inhibitor, can effectively inhibit the growth of the osteosarcoma cell lines in vitro. It can also disrupt the development of tumors in the patient-derived xenograft (PDX) models in vivo. Furthermore, we confirmed that the mode of action (MoA) of volasertib is primarily mediated by the cell-cycle arrest and apoptosis triggered by DNA damage. As PLK1 inhibitors are entering phase III clinical trials, our findings provide important insights into the efficacy and MoA of the relevant therapeutic approach for combating osteosarcoma.
Structural insights into RNA bridging between HIV-1 Vif and antiviral factor APOBEC3G
Authors: Kouno Takahide , Shibata Satoshi , Shigematsu Megumi , Hyun Jaekyung , Kim Tae Gyun , Matsuo Hiroshi , Wolf Matthias 
Published: July 2023

AbstractGreat effort has been devoted to discovering the basis of A3G-Vif interaction, the key event of HIV’s counteraction mechanism to evade antiviral innate immune response. Here we show reconstitution of the A3G-Vif complex and subsequent A3G ubiquitination in vitro and report the cryo-EM structure of the A3G-Vif complex at 2.8 Å resolution using solubility-enhanced variants of A3G and Vif. We present an atomic model of the A3G-Vif interface, which assembles via known amino acid determinants. This assembly is not achieved by protein-protein interaction alone, but also involves RNA. The cryo-EM structure and in vitro ubiquitination assays identify an adenine/guanine base preference for the interaction and a unique Vif-ribose contact. This establishes the biological significance of an RNA ligand. Further assessment of interactions between A3G, Vif, and RNA ligands show that the A3G-Vif assembly and subsequent ubiquitination can be controlled by amino acid mutations at the interface or by polynucleotide modification, suggesting that a specific chemical moiety would be a promising pharmacophore to inhibit the A3G-Vif interaction.
Atypical splicing variants in PKD1 explain most undiagnosed typical familial ADPKD
Authors: Hort Yvonne , Sullivan Patricia , Wedd Laura , Fowles Lindsay , Stevanovski Igor , Deveson Ira , Simons Cas , Mallett Andrew , Patel Chirag , Furlong Timothy , Cowley Mark J. , Shine John , Mallawaarachchi Amali 
Published: July 2023

AbstractAutosomal dominant polycystic kidney disease (ADPKD) is the most common monogenic cause of kidney failure and is primarily associated with PKD1 or PKD2. Approximately 10% of patients remain undiagnosed after standard genetic testing. We aimed to utilise short and long-read genome sequencing and RNA studies to investigate undiagnosed families. Patients with typical ADPKD phenotype and undiagnosed after genetic diagnostics were recruited. Probands underwent short-read genome sequencing, PKD1 and PKD2 coding and non-coding analyses and then genome-wide analysis. Targeted RNA studies investigated variants suspected to impact splicing. Those undiagnosed then underwent Oxford Nanopore Technologies long-read genome sequencing. From over 172 probands, 9 met inclusion criteria and consented. A genetic diagnosis was made in 8 of 9 (89%) families undiagnosed on prior genetic testing. Six had variants impacting splicing, five in non-coding regions of PKD1. Short-read genome sequencing identified novel branchpoint, AG-exclusion zone and missense variants generating cryptic splice sites and a deletion causing critical intron shortening. Long-read sequencing confirmed the diagnosis in one family. Most undiagnosed families with typical ADPKD have splice-impacting variants in PKD1. We describe a pragmatic method for diagnostic laboratories to assess PKD1 and PKD2 non-coding regions and validate suspected splicing variants through targeted RNA studies.
Quantum reservoir computing implementation on coherently coupled quantum oscillators
Authors: Dudas Julien , Carles Baptiste , Plouet Erwan , Mizrahi Frank Alice , Grollier Julie , Marković Danijela 
Published: July 2023

AbstractQuantum reservoir computing is a promising approach for quantum neural networks, capable of solving hard learning tasks on both classical and quantum input data. However, current approaches with qubits suffer from limited connectivity. We propose an implementation for quantum reservoir that obtains a large number of densely connected neurons by using parametrically coupled quantum oscillators instead of physically coupled qubits. We analyze a specific hardware implementation based on superconducting circuits: with just two coupled quantum oscillators, we create a quantum reservoir comprising up to 81 neurons. We obtain state-of-the-art accuracy of 99% on benchmark tasks that otherwise require at least 24 classical oscillators to be solved. Our results give the coupling and dissipation requirements in the system and show how they affect the performance of the quantum reservoir. Beyond quantum reservoir computing, the use of parametrically coupled bosonic modes holds promise for realizing large quantum neural network architectures, with billions of neurons implemented with only 10 coupled quantum oscillators.
Morphomigrational description as a new approach connecting cell's migration with its morphology
Authors: Kołodziej Tomasz , Mielnicka Aleksandra , Dziob Daniel , Chojnacka Anna Katarzyna , Rawski Mateusz , Mazurkiewicz Jan , Rajfur Zenon 
Published: July 2023

AbstractThe examination of morphology and migration of cells plays substantial role in understanding the cellular behaviour, being described by plethora of quantitative parameters and models. These descriptions, however, treat cell migration and morphology as independent properties of temporal cell state, while not taking into account their strong interdependence in adherent cells. Here we present the new and simple mathematical parameter called signed morphomigrational angle (sMM angle) that links cell geometry with translocation of cell centroid, considering them as one morphomigrational behaviour. The sMM angle combined with pre-existing quantitative parameters enabled us to build a new tool called morphomigrational description, used to assign the numerical values to several cellular behaviours. Thus, the cellular activities that until now were characterized using verbal description or by complex mathematical models, are described here by a set of numbers. Our tool can be further used in automatic analysis of cell populations as well as in studies focused on cellular response to environmental directional signals.
Vocalizations of wild West Indian manatee vary across subspecies and geographic location
Authors: Reyes-Arias Jessica D. , Brady Beth , Ramos Eric A. , Henaut Yann , Castelblanco-Martínez Delma Nataly , Maust-Mohl Maria , Searle Linda , Pérez-Lachaud Gabriela , Guzmán Héctor M. , Poveda Héctor , Merchan Fernando , Contreras Kenji , Sanchez-Galan Javier E. , Collom Kristi A. , Magnasco Marcelo O. 
Published: July 2023

AbstractGeographic variation in the vocal behavior of manatees has been reported but is largely unexplored. Vocalizations of wild West Indian manatees (Trichechus manatus) were recorded with hydrophones in Florida from Florida manatees (Trichechus manatus latirostris), and in Belize and Panama from Antillean manatees (Trichechus manatus manatus) to determine if calls varied between subspecies and geographic regions. Calls were visually classified into five categories: squeaks, high squeaks, squeals, squeak-squeals, and chirps. From these five categories, only three call types (squeaks, high squeaks and squeals) were observed in all three populations. Six parameters from the temporal and frequency domains were measured from the fundamental frequency of 2878 manatee vocalizations. A repeated measures PERMANOVA found significant differences for squeaks and high squeaks between each geographic location and for squeals between Belize and Florida. Almost all measured frequency and temporal parameters of manatee vocalizations differed between and within subspecies. Variables that may have influenced the variation observed may be related to sex, body size, habitat and/or other factors. Our findings provide critical information of manatee calls for wildlife monitoring and highlight the need for further study of the vocal behavior of manatees throughout their range.
Ceramide releases exosomes with a specific miRNA signature for cell differentiation
Authors: Fiorani Federico , Domenis Rossana , Dalla Emiliano , Cataldi Samuela , Conte Carmela , Mandarano Martina , Sidoni Angelo , Cifù Adriana , Beccari Tommaso , Mirarchi Alessandra , Arcuri Cataldo , Curcio Francesco , Albi Elisabetta 
Published: July 2023

AbstractExosomes are well established effectors of cell–cell communication. Their role on maturation of embryonic cells located in hippocampus, seat of memory, is unknown. Here we show that ceramide facilitates release of exosomes from HN9.10e cells extending information for cell differentiation to neighboring cells. We found only 38 miRNAs differentially expressed in exosomes derived from ceramide-treated cells in comparison with control cells (including 10 up-regulated and 28 down-regulated). Some overexpressed miRNAs (mmu-let-7f-1-3p, mmu-let-7a-1-3p, mmu-let-7b-3p, mmu-let-7b-5p, mmu-miR-330-3p) regulate genes encoding for protein involved in biological, homeostatic, biosynthetic and small molecule metabolic processes, embryo development and cell differentiation, all phenomena relevant for HN9.10e cell differentiation. Notably, the overexpressed mmu-let-7b-5p miRNA appears to be important for our study based on its ability to regulate thirty-five gene targets involved in many processes including sphingolipid metabolism, sphingolipid-related stimulation of cellular functions and neuronal development. Furthermore, we showed that by incubating embryonic cells with exosomes released under ceramide treatment, some cells acquired an astrocytic phenotype and others a neuronal phenotype. We anticipate our study to be a start point for innovative therapeutic strategies to regulate the release of exosomes useful to stimulate delayed brain development in the newborn and to improve the cognitive decline in neurodegenerative disorders.