bluuebunny/crossref_metadata_2025_split huggingface dataset:
Identification and validation of BCL6 and VEGFA as biomarkers and ageing patterns correlating with immune infiltrates in OA progression
Authors: Chen Ziyi , Wang Wenjuan , Hua Yinghui 
Published: February 2023

AbstractOsteoarthritis (OA), the most common type of arthritis, is a complex biological response caused by cartilage wear and synovial inflammation that links biomechanics and inflammation. The progression of OA correlates with a rise in the number of senescent cells in multiple joint tissues. However, the mechanisms by which senescent cells and their involvement with immune infiltration promote OA progression are not fully understood. The gene expression profiles and clinical information of OA and healthy control synovial tissue samples were retrieved from the Gene Expression Omnibus database, and then differential analysis of senescence regulators between OA and normal samples was performed. The random forest (RF) was used to screen candidate senescence regulators to predict the occurrence of OA. The reverse transcription quantitative real-time PCR experiments at tissue’s level was performed to confirm these biomarkers. Moreover, two distinct senescence patterns were identified and systematic correlation between these senescence patterns and immune cell infiltration was analyzed. The senescence score and senescence gene clusters were constructed to quantify senescence patterns together with immune infiltration of individual OA patient. 73 senescence differentially expressed genes were identified between OA patients and normal controls. The RF method was utilized to build an OA risk model based on two senescence related genes: BCL6 and VEGFA. Next, two distinct aging patterns were determined in OA synovial samples. Most patients from senescence cluster A were further classified into gene cluster B and high senescence score group correlated with a non-inflamed phenotype, whereas senescence cluster B were classified into gene cluster A and low senescence score group correlated with an inflamed phenotype. Our study revealed that senescence played an important role in in OA synovial inflammation. Evaluating the senescence patterns of individuals with OA will contribute to enhancing our cognition of immune infiltration characterization, providing novel diagnostic and prognostic biomarkers, and guiding more effective immunotherapy strategies.
Identification of novel targets and pathways to distinguish suicide dependent or independent on depression diagnosis
Authors: Peng Siqi , Zhou Yalan , Xiong Lan , Wang Qingzhong 
Published: February 2023

AbstractIn recent years, postmortem brain studies have revealed that some molecular, cellular, and circuit changes associated with suicide, have an independent or additive effect on depression. The aim of the present study is to identify potential phenotypic, tissue, and sex-specific novel targets and pathways to distinguish depression or suicide from major depressive disorder (MDD) comorbid with suicide. The mRNA expression profiling datasets from two previous independent postmortem brain studies of suicide and depression (GSE102556 and GSE101521) were retrieved from the GEO database. Machine learning analysis was used to differentiate three regrouped gene expression profiles, i.e., MDD with suicide, MDD without suicide, and suicide without depression. Weighted correlation network analysis (WGCNA) was further conducted to identify the key modules and hub genes significantly associated with each of these three sub-phenotypes. TissueEnrich approaches were used to find the essential brain tissues and the difference of tissue enriched genes between depression with or without suicide. Dysregulated gene expression cross two variables, including phenotypes and tissues, were determined by global analysis with Vegan. RRHO analysis was applied to examine the difference in global expression pattern between male and female groups. Using the optimized machine learning model, several ncRNAs and mRNAs with higher AUC and MeanDecreaseGini, including GCNT1P1 and AC092745.1, etc., were identified as potential molecular targets to distinguish suicide with, or without MDD and depression without suicide. WGCNA analysis identified some key modules significantly associated with these three phenotypes, and the gene biological functions of the key modules mainly relate to ncRNA and miRNA processing, as well as oxidoreductase and dehydrogenase activity. Hub genes such as RP11-349A22.5, C20orf196, MAPK8IP3 and RP11-697N18.2 were found in these key modules. TissueEnrich analysis showed that nucleus accumbens and subiculum were significantly changed among the 6 brain regions studied. Global analysis with Vegan and RRHO identified PRS26, ARNT and SYN3 as the most significantly differentially expressed genes across phenotype and tissues, and there was little overlap between the male and female groups. In this study, we have identified novel gene targets, as well as annotated functions of co-expression patterns and hub genes that are significantly distinctive between depression with suicide, depression without suicide, and suicide without depression. Moreover, global analysis across three phenotypes and tissues confirmed the evidence of sex difference in mood disorders.
In vivo determination of domestic quail (Coturnix japonica) muscle development by ultrasonography as a function of energy levels
Authors: Santos Thailine Santana , Alves Isis Nayara Santana , dos Santos Araujo Jucélia , de Assis Ananda Santos , Ribeiro Júnior Valdir , Brito Claudson Oliveira , Silva Camilla Mendonça , Santos Anselmo Domingos Ferreira , Barbosa Leandro Teixeira , Zancanela Vittor Tuzzi , de Oliveira Júnior Gregório Murilo 
Published: February 2023

AbstractThe aim was to evaluate the growth and meat production and some meat quality characteristics of domestic quail (Coturnix japonica) as a function of metabolizable energy (ME) levels in the diet and to adjust predicting equations in ratio to area pectoralis major muscle of the carcass through the ultrasound. Two hundred and seventy mixed sex quail from 7 to 49 days old were distributed in three treatments (7 to 21 days old, diets with 2700; 2900 and 3100 kcal ME/kg; from 21 to 49 days, diets with 2900, 3050 and 3200 kcal of ME/kg), with five replications per treatment. Ultrasonography was performed at 21; 35 and 49 days of age in the pectoralis major muscle to determine prediction equations through multiple linear regression. Feed intake (FI) and feed efficiency (FE) were linearly influenced (P < 0.05) by energy levels in both phases evaluated. Regarding sensory analysis, there was no interference of ME levels on sensory attributes. The equation for predicting breast area was Y = 0.00271*ME + 0.25411*Age-9.58002, R2 = 74.25%. It is concluded that increasing the energy level of the feed from 2700 to 3100 kcal ME/kg reduces FI and improves the FE of quail. The increase in carcass fat from 35 days of age does not harm the physical and sensory characteristics of the meat. The ultrasonography in vivo of the M. pectoralis major, considering the age and energy level of the diet, made it possible to predict the pectoral muscle are a with higher reliability.
Evaluating red tide effects on the West Florida Shelf using a spatiotemporal ecosystem modeling framework
Authors: Vilas Daniel , Buszowski Joe , Sagarese Skyler , Steenbeek Jeroen , Siders Zach , Chagaris David 
Published: February 2023

AbstractThe West Florida Shelf (WFS), located in the eastern Gulf of Mexico, fosters high species richness and supports highly valuable fisheries. However, red tide events occur regularly that can impact fisheries resources as well as ecosystem state, functioning, and derived services. Therefore, it is important to evaluate and quantify the spatiotemporal impacts of red tides to improve population assessments, mitigate potential negative effects through management, and better understand disturbances to support an ecosystem-based management framework. To model red tide effects on the marine community, we used Ecospace, the spatiotemporal module of the ecosystem modeling framework Ecopath with Ecosim. The inclusion of both lethal and sublethal response functions to red tide and a comprehensive calibration procedure allowed to systematically evaluate red tide effects and increased the robustness of the model and management applicability. Our results suggest severe red tide impacts have occurred on the WFS at the ecosystem, community, and population levels in terms of biomass, catch, and productivity. Sublethal and indirect food-web effects of red tide triggered compensatory responses such as avoidance behavior and release from predation and/or competition.. This study represents a step forward to operationalize spatiotemporal ecosystem models for management purposes that may increase the ability of fisheries managers to respond more effectively and be more proactive to episodic mortality events, such as those caused by red tides.
Adélie penguins north and east of the ‘Adélie gap’ continue to thrive in the face of dramatic declines elsewhere in the Antarctic Peninsula region
Authors: Wethington Michael , Flynn Clare , Borowicz Alex , Lynch Heather J. 
Published: February 2023

AbstractWhile population declines among Adélie penguins and population increases among gentoo penguins on the Western Antarctic Peninsula are well established, the logistical challenges of operating in the sea ice-heavy northern tip of the Antarctic Peninsula have prohibited reliable monitoring of seabirds in this region. Here we describe the findings of an expedition to the northern and eastern sides of the Antarctic Peninsula—a region at the nexus of two proposed Marine Protected Areas—to investigate the distribution and abundance of penguins in this region. We discovered several previously undocumented penguin colonies, completed direct surveys of three colonies initially discovered in satellite imagery, and re-surveyed several colonies last surveyed more than a decade ago. Whereas our expectation had been that the Peninsula itself would divide the areas undergoing ecological transition and the apparently more stable Weddell Sea region, our findings suggest that the actual transition zone lies in the so-called "Adélie gap," a 400-km stretch of coastline in which Adélies are notably absent. Our findings suggest that the region north and east of this gap represents a distinct ecoregion whose dynamics stand in sharp contrast to surrounding areas and is likely to be impacted by future conservation measures.
Effects of thinning on soil nutrient availability and fungal community composition in a plantation medium-aged pure forest of Picea koraiensis
Authors: Caihong Zhao , Nier Su , Hao Wang , Honglin Xing , Hailong Shen , Ling Yang 
Published: February 2023

AbstractThinning is an important silvicultural practice for improving the productivity and wood production in plantation forest. Different intensities of thinning management can affect tree growth and alter soil nutrient effectiveness, thus affecting soil fungal community structure and diversity. Our objective is to determine the soil factors and their regulatory mechanisms that influence stand growth by thinning, and to provide data to support the establishment of large diameter timber cultivation technology for Picea koraiensis. In this study, we conducted medium- and high-intensity thinning in 43a P. koraiensis plantation middle-aged forests and investigated the growth indexes, soil physicochemical properties, and fungal community diversity in rhizosphere and non-rhizosphere soils of the stands after thinning at different densities (904 plants/ha for control, 644 plants/ha for 30% thinning intensity, and 477 plants/ha for 50% thinning intensity). The results showed that all growth indicators (annual growth of tree height, diameter at breast height, height under live branches and crown width) of the plantation after high-intensity thinning (477 plants/ha) were higher than those of the control (no thinning, significant) and medium-intensity thinning (644 plants/ha). Mycorrhizal infection rate was higher at the beginning of the growing season than at the end of the growing season, and increased slightly with decreasing stand density. Compared to the control, all medium- and high-intensity thinning treatments significantly improved soil nutrient content (P < 0.05), including total carbon, total nitrogen, total phosphorus, total potassium, Available phosphorus and Available potassium. Fungal diversity was higher but lower in abundance than the control in both rhizosphere and non-rhizosphere soils after thinning. The number of OTUs and fungal richness and diversity indices of non-rhizosphere soil fungi were higher than those of rhizosphere soil fungi. In conclusion, this study provides new evidence that reasonable intercalation can increase the radial and vertical growth of P. koraiensis plantation forests and promote the diversity of subsurface soil fungal communities. It is shown that thinning intensity regulates biogeochemical cycles in P. koraiensis plantation ecosystems by affecting soil nutrients and fungal community structure. Therefore, 50% thinning intensity can be used to increase timber production in plantation forests during large diameter timber cultivation of P. koraiensis and improve predictions associated with achieving long-term forest management strategies.
Associations among perceived health competence, effortful control, self-control, and personality traits in Japanese university students
Authors: Yoshioka Takuma , Ohara Kumiko , Momoi Katsumasa , Mase Tomoki , Nakamura Harunobu 
Published: February 2023

AbstractPerceived health competence is thought to contribute to lifelong healthy behavior. However, the factors that affect perceived health competence have not been investigated. We investigated the associations among perceived health competence, effortful control, self-control, and personality traits in university students and proposed a model of how these factors affect perceived health competence. The participants were 320 Japanese university students who completed a questionnaire regarding their height, weight, perceived health competence, effortful control, self-control, and personality traits. The three-step multiple regression analysis showed that effortful control was positively associated with the perceived health competence, and self-control was positively with, and impulsivity was inversely associated with effortful control respectively, indicating that effortful control was an intermediate factor. Structural equation modeling showed a good fit for both genders, with a common path for both genders to perceived health competence via effortful control and a different involvement of personality traits for men and women. These results suggest that effortful control is directly associated with perceived health competence; in addition, both self-control and impulsiveness are indirectly associated with perceived health competence via effortful control.
Predicting the prevalence of complex genetic diseases from individual genotype profiles using capsule networks
Authors: Luo Xiao , Kang Xiongbin , Schönhuth Alexander 
Published: February 2023

AbstractDiseases that have a complex genetic architecture tend to suffer from considerable amounts of genetic variants that, although playing a role in the disease, have not yet been revealed as such. Two major causes for this phenomenon are genetic variants that do not stack up effects, but interact in complex ways; in addition, as recently suggested, the omnigenic model postulates that variants interact in a holistic manner to establish disease phenotypes. Here we present DiseaseCapsule, as a capsule-network-based approach that explicitly addresses to capture the hierarchical structure of the underlying genome data, and has the potential to fully capture the non-linear relationships between variants and disease. DiseaseCapsule is the first such approach to operate in a whole-genome manner when predicting disease occurrence from individual genotype profiles. In experiments, we evaluated DiseaseCapsule on amyotrophic lateral sclerosis (ALS) and Parkinson’s disease, with a particular emphasis on ALS, which is known to have a complex genetic architecture and is affected by 40% missing heritability. On ALS, DiseaseCapsule achieves 86.9% accuracy on hold-out test data in predicting disease occurrence, thereby outperforming all other approaches by large margins. Also, DiseaseCapsule required sufficiently less training data for reaching optimal performance. Last but not least, the systematic exploitation of the network architecture yielded 922 genes of particular interest, and 644 ‘non-additive’ genes that are crucial factors in DiseaseCapsule, but remain masked within linear schemes.
Unusual shrinkage and reshaping of Earth’s magnetosphere under a strong northward interplanetary magnetic field
Authors: Wang Xiang-Yu , Zhang Qing-He , Wang Chi , Zhang Yong-Liang , Tang Bin-Bin , Xing Zan-Yang , Oksavik Kjellmar , Lyons Larry R. , Lockwood Michael , Zong Qiu-Gang , Li Guo-Jun , Liu Jing , Ma Yu-Zhang , Wang Yong 
Published: February 2023

AbstractThe Earth’s magnetosphere is the region of space where plasma behavior is dominated by the geomagnetic field. It has a long tail typically extending hundreds of Earth radii (RE) with plentiful open magnetic fluxes threading the magnetopause associated with magnetic reconnection and momentum transfer from the solar wind. The open-flux is greatly reduced when the interplanetary magnetic field points northward, but the extent of the magnetotail remains unknown. Here we report direct observations of an almost complete disappearance of the open-flux polar cap characterized by merging poleward edges of a conjugate horse-collar aurora (HCA) in both hemispheres’ polar ionosphere. The conjugate HCA is generated by particle precipitation due to Kelvin-Helmholtz instability in the dawn and dusk cold dense plasma sheets (CDPS). These CDPS are consist of solar wind plasma captured by a continuous dual-lobe magnetic reconnections, which is further squeezed into the central magnetotail, resulting in a short “calabash-shaped” magnetotail.
Examining the Relation Between Exercise and Word Learning in Preschool-Age Children
Authors: Mellor Lauren , Morini Giovanna 
Published: February 2023

Purpose:
There is evidence suggesting that aerobic exercise immediately after vocabulary training can improve word recall in school-age children. This work examined whether the previously identified word-learning benefits associated with exercise can be extended to preschoolers. Additionally, we evaluated whether the effects of physical activity on vocabulary learning may be influenced by existing language skills that the child possesses.

Method:

Children ages 3–6 years completed the study (
N
= 42). Data were collected via a virtual testing session in which participants completed a word-learning task that included two experimental conditions (
exercise
and
resting
). In the resting measure, children were taught names of novel objects and then sat down and colored for 3 min before being tested on their ability to identify the trained words. The exercise condition was identical, except that participants engaged in 3 min of guided aerobic exercise before testing. Additionally, at the end of the visit, participants completed the Quick Interactive Language Screener (QUILS), which measured general language skills.

Results:
Accuracy of word recognition was significantly higher after the exercise condition compared to the resting condition. Furthermore, this pattern of results was not related to children's existing language skills, as measured by the QUILS.

Conclusions:
This study is one of the first to closely examine the relation between physical activity and word-learning abilities in children as young as 3–6 years of age. Results align with previous findings stating that aerobic exercise can boost vocabulary learning and suggest that this is the case regardless of existing language skills.
Adaptive classification helps hybrid visual brain computer interface systems handle non‐stationary cortical signals
Authors: Kapgate Deepak D. , Prasad. K Krishna 
Published: February 2023

AbstractThe classifier efficiency of the brain‐computer interface systems is significantly impacted by the non‐stationarity of electroencephalogram (EEG) signals. We propose an adaptive variant of the linear discriminant analysis (LDA) classifier as a solution to this problem. This classifier constantly adjusts its parameters to account for the most recent EEG data. In this study, the authors will update the mean values as well as the covariance matrix of each class pair. Visually evoked cortical potential datasets are used to check how well the proposed classifier performs. The authors prove that the proposed adaptive LDA performs much better than both static multiclass LDA and adaptive PMean LDA.
Autoimmunologische Kleinhirnerkrankungens
Authors: Vogel Niklas , Hartmann Christian , Meuth Sven , Melzer Nico 
Published: February 2023

ZUSAMMENFASSUNGDie autoimmunologischen Kleinhirnerkrankungen umfassen paraneoplastische wie die paraneoplastische Kleinhirndegeneration oder das Opsoklonus-Myoklonus-Syndrom mit zerebellärer Ataxie und nicht paraneoplastische Ursachen wie die Gluten-Ataxie oder die postinfektiöse Cerebellitis. Klinisch eint die unterschiedlichen Ätiologien eine führende Gang- und Standataxie. Eine zentrale Rolle in der Diagnostik spielen antineuronale Antikörper, die einen Hinweis auf die ursächliche Grunderkrankung geben können. Es werden fortlaufend neue Antikörper entdeckt, die in einem möglichen Zusammenhang mit der zerebellären Ataxie stehen. Onkoneuronale Antikörper können bei der Bestimmung der zugrunde liegenden Tumorentität und der Prognoseabschätzung eine Hilfestellung sein. Die Basistherapie richtet sich nach der primären Ursache, z. B. Tumortherapie oder glutenfreie Diät. Alternativ stehen Kortikosteroide, IVIG, Plasmaseparation und Immunsuppressiva wie Rituximab und/oder Cyclophosphamid zur Verfügung.
Schlüsselprinzip Bindegewebe
Authors: Corts Magga 
Published: February 2023

SummaryDas aus dem Mesoderm entstandene Bindegewebe bringt als „Mutter“ vieler Gewebetypen Muskeln, Sehnen, Bänder, Blutzellen und die Interzellularsubstanz hervor und dient als Wasserspeicher, Hüter der metabolischen Homöostase und Immunregulans. Hypothalamus, Thalamus und limbisches System sind über diverse Regelkreise ebenso an der Geweberegulation beteiligt wie Sympathikus und Parasympathikus – somit lassen sich lokale Strukturen gut vegetativ ansprechen. Muskeln, Sehnen und Bänder zählen zu den Faszien als Subtyp des Bindegewebes und verfügen somit über eine hochsensible neuronale Versorgung über Mechanorezeptoren – mit daraus entspringenden manuellen Therapieoptionen.
Polymyalgia rheumatica und Riesenzellarteriitis
Authors: Noschinski Dirk-Rüdiger 
Published: February 2023

SummaryDie Zahl von Autoimmunerkrankungen nimmt insgesamt zu – ebenso die Zahl der Patienten, die gleichzeitig an mehreren Autoimmunerkrankungen leiden. Ist die Polymyalgia rheumatica eine abgemilderte Form der Riesenzellarteriitis oder handelt es sich um zwei getrennte Krankheitsbilder? Welche Differenzialdiagnosen kommen in Betracht? Die Naturheilkunde kann mit adjuvanter Schubbegleitung, etwa durch die Verabreichung von „anthroposophischem Kortison“, und Rezidivprophylaxe helfen
Schmerzen in der Hüftregion
Authors: Delarue Cyril André 
Published: February 2023

SummaryInstabilitäten der unteren Extremitäten können auf Dauer oder bei Anstrengung zu einem Piriformis-Syndrom führen. Das geschilderte Fallbeispiel zeigt: Auch sportliche Menschen sind betroffen. Es ist nötig, grundlegende Bewegungsabläufe zu testen und die Defizite zu deuten, um die Beschwerden erfolgreich zu therapieren.