bluuebunny/crossref_metadata_2025_split huggingface dataset:
Peculiar Transient Behaviors of Organic Electrochemical Transistors Governed by Ion Injection Directionality
Authors: Yoon Myung-Han , Kim Ji Hwan , Halaksa Roman , Jo Il-Young , Ahn Hyungju , Finn Peter , Lee Inho , Park Sungjun , Nielsen Christian 
Published: May 2023

Abstract
Despite the growing interest in dynamic behaviors at the frequency domain, there exist very few studies on molecular orientation-dependent transient responses of organic mixed ionic–electronic conductors. In this research, we investigated the effect of ion injection directionality on transient electrochemical transistor behaviors by developing a model mixed conductor system. Two polymers with similar electrical, ionic, and electrochemical characteristics but distinct molecular orientations were successfully synthesized by varying the co-monomer unit (2,2’-bithiophene or phenylene) in conjunction with a novel 1,4-dithienylphenylene-based monomer. The comprehensive electrochemical analysis suggests that the molecular orientation affects the length of the ion-drift pathway, which is directly correlated with ion mobility, resulting in peculiar OECT transient responses. These results provide the general insight into molecular orientation-dependent ion movement characteristics as well as high-performance device design principles with fine-tuned transient responses.
Psychological mechanisms of willingness to participate in high-value markets: an eye-tracking experiment
Authors: Liu Chao , Lei Hongzhen , Lan Juanli , Li Fan , Liu Ye 
Published: May 2023

Abstract
The high-value markets (HVMs) plays an important role in facilitating stable incomes and sustainable livelihoods for farmers. However, the existing analysis mostly focus on the external and environmental characteristics, ignoring the internal resource endowment of the information processing ability of farmers. Based on the cognitive resource, eye movement experiments were implemented to investigate the psychological mechanisms underlying the willingness to participate in HVMs. Empirical results suggested that cognitive load increases individual’s preference for earlier consumption and further weakens willingness to participate in the HVMs. Combined with eye movement recorded by eye-tracking, the possible path that cognitive load indirectly affects time preference by distracting farmers' attention and weakening their self-control ability were excluded. This study provides new ideas for deepening the understanding of HVMs development and enhancing the level of vertical cooperation in production and marketing links.
Y4O2(CN2)3Cl2, its relation to the metal-rich Y2Cl3, and the photoluminescence of Y4O2(CN2)3Cl2:Ce
Authors: Brand Catharina-Darine , Ströbele Markus , Enseling David , Jüstel Thomas , Meyer Hans-Jürgen 
Published: May 2023

Abstract
Y4O2(CN2)3Cl2 and some isotypic rare earth (RE) carbodiimides RE4O2(CN2)3Cl2 (RE = Sm, Gd, Tb) were prepared by solid-state metathesis reaction between RECl3, REOCl and Li2CN2. The crystal structure of Y4O2(CN2)3Cl2 was solved and refined from single-crystal XRD data with the monocline space group C2/m. Homologous compounds with RE = Sm, Gd were assigned isotopically by means of XRD powder diffraction. The structure of Y4O2(CN2)3Cl2 is characterized by one-dimensional cluster chains which are showing a striking resemblance to the trans-edge bridging RE6-octahedra in the structures of Y2Cl3, β-Y2Cl3N, and RE2N(CN2)Cl, despite different numbers of cluster electrons. Crystals of RE4O2(CN2)3Cl2 appear as transparent rods. The presence of (NCN)2− ions is confirmed by infrared spectroscopy. Y4O2(CN2)3Cl2, doped with Ce3+, is a photoluminescent material showing an emission band in the orange region (λem = 550 nm) of the visible spectrum.
gmXtal: Cooking crystals with GROMACS
Authors: Buslaev Pavel , Groenhof Gerrit 
Published: May 2023

Abstract
Molecular dynamics (MD) simulations are routinely performed of biomolecules in solution, because this is their native environment. However, the structures used in such simulations are often obtained with x-ray crystallography, which provides the atomic coordinates of the biomolecule in a crystal environment. With the advent of free electron lasers, x-ray crystallography can also access the dynamics in crystals. Such experiments provide the possibility to directly verify the validity of an MD simulation. A direct comparison requires that the simulation of the biomolecule is also performed in the crystal environment. However, in contrast to simulations of biomolecules in solution, setting up a crystal is challenging. In particular, because not all solvent molecules are resolved in x-ray crystallography, adding an appropriate number of solvent molecules can be difficult and typically is a trial-and-error based procedure requiring manual interventions. Such interventions preclude high throughput applications. To overcome this bottleneck, we introduce gmXtal, a tool for setting up crystal simulations for MD simulations with GROMACS. With the information from the protein data bank rcsb.org gmXtal automatically (i) builds the unit cell; (ii) sets the protonation of titratable residues; (iii) builds missing residues that were not resolved experimentally; and (iv) adds an appropriate number of solvent molecules to the system. gmXtal is available as a standalone tool https://gitlab.com/pbuslaev/gmxtal.
Machinability of Cu-Al-Mn Shape Memory Alloys
Authors: Hong Huanpeng , Gencturk Bora , Araki Yoshikazu , Saiidi Saiid , Kise Sumio 
Published: May 2023

Abstract

Cu-Al-Mn (CAM) shape memory alloys (SMA) are cost effective, have a high low-cycle fatigue life and superelastic limit, and a wide temperature application range compared to other types of SMAs. These characteristics of CAM SMAs have resulted in an increased research interest in their use in civil engineering applications, particularly as reinforcement in concrete structures, and dampers in steel structures. However, these applications could require machining of the CAM SMA bars for connecting with other structural elements. This study presents the methods and results of the first systematic research on the machinability of CAM SMAs. The key machinability characteristics of CAM SMAs, such as chip formation, cutting temperature, tool wear, workpiece surface roughness and diameter deviation were studied and compared with conventional NiTi SMAs, and commonly used steel: mild steel (MS) and 304 stainless steel (SS). Effects of a wide range of cutting parameters, such as cutting speed ranging from 15 to 120 m/min, feed rate ranging from 0.1 to 0.2 mm/rev, and depth of cut ranging from 0.5 to 1.5 mm, were investigated. The results from this study demonstrated that the tool wear from machining CAM SMAs was close to that of SS and slightly higher than that from machining MS but much lower than of that from machining NiTi SMAs. In all the cases considered here, the tool wear from machining CAM SMAs was found to be 0.6 to 1.8 times that from machining SS, 0.8 to 2.4 times that from machining MS, and 1/7 to 1/21 times that from machining NiTi SMAs. After a continuous machining test with a total cutting length of 4.5 m, the nose wear of machining CAM SMAs was found to be 1.6 times that of machining MS, and the average flank wear of machining CAM SMAs was found to be three times that of machining MS; the diameter deviation (relative diameter difference with the first sample) of CAM SMAs was only 10 mm larger than that of MS.
Endophytic Acrocalymma vagum drives the microbiome variations for rice health
Authors: Zeng Yu-Lan , Lu Xuan-Jun , Wang Meng-Rong , Zhu Jia-Nan , Li Qian-Xi , Zhu Qin-Ping , Wang Ling , Bai Zhen-Feng , Liu Xiao-Hong , Su Zhen-Zhu , Lin Fu-Cheng 
Published: May 2023

Abstract
Background: Endophytic fungi are essential members of the plant microbial community, which play a vital role in promoting plant growth and development, and inducing disease resistance. However, little is known about the ecological mechanisms of endophytic fungi impacting the root microbiota community to improve crop health.
Results: An endophytic fungus Acrocalymma vagum (Code A21) isolated from wild rice enhanced rice yield by 5.73% and induced resistance against rice blast with 83.24% control efficiency in the field. The root microbiota community variations were discovered through 16S and ITS metabarcoding methods. A. vagum simplified the complexity of the rice microbiome community, reducing the proportion of the dominant pathogens Burkholderia and Ralstonia, and driving rice to recruit beneficial bacteria (Aquabacterium, Flavobacterium, and Lactobacillus) and fungi (Rhizophagus, Sarocladium, and Nigrospora). The recruited microbes were enriched in nutrient synthesis and stress resistance, especially biosynthesis of lactate, vitamin B12, and thiazole. We further isolated and identified 279 bacteria and 82 fungi from the A. vagum-fertilized rice roots to confirm the probiotic's functions. Among them, 5 Lactobacillus strains and 2 Nigrospora strains possessed significantly inhibitive activity on rice blast pathogen Magnaporthe oryzae. Additionally, 3 fungal strains in Sarocladium and Nigrospora genera promoted rice growth. Mutualistic mechanisms of A. vagum-rice were uncovered by the LC-MS/MS-based label-free quantitative assay. 13 secretory proteins of A. vagum cooperated to evade the host immune response and stimulate the symbiont resistance, such as alkaline phosphatases (ALP) as specific marker enzymes to symbiosis with the host and glycoside hydrolases (GHs) as effectors to colonize the host. Correspondingly, 206 rice proteins were induced to up-regulate, especially families of peroxidase (OsPRX), copper transporter (OsCOPT), and glutathione S-transferase (OsGSTU) related to growth and stress resistance.
Conclusion: Our study verified that endophytic fungi isolated from wild rice had great practical value on cultivated rice. A. vagum reshaped the microbiota community of cultivated rice by enhancing host traits, as the first report of simplification and optimization effect of endophytic fungi on plant recruiting microbes, which offers novel perspectives on using endophytic fungi to increase agricultural productivity.
Fecal microbiota transplantation for Parkinson's patients with constipation: A pilot clinical trial
Authors: Qiu Zhibing , Zhang Huilu , Shen Cong , Liu Jie , Wang Jian , Zhang Jun 
Published: May 2023

Abstract
Background Gut dysbacteriosis is closely related to Parkinson's disease. Fecal microbiota transplantation (FMT) has been proposed as an effective way to reconstruct gut microbiota. We launched a clinical study to examine the safety and efficacy of FMT for Parkinson’s disease.Methods Five Parkinson's patients with constipation aged 55 to 71 years were enrolled in this prospective open-label study. Patients received FMT on 3 consecutive days through nasojejunal tubes and were followed up for 8 weeks after treatment. The UPDRS-III OFF score was used to evaluate the improvement in neurological symptoms, and the Wexner score was used to evaluate the improvement in constipation. Shotgun metagenomic sequencing of fecal samples to compare the microbial communities before and after FMT. Fecal and serum samples from patients before and after FMT at the indicated times were subjected to metabolomics for metabolites.Results FMT improved constipation and neurological symptoms in Parkinson's patients. Bifidobacterium bifidum, Anaerotruncus colihomini, Acinetobacter sp., Proteobacteria bacterium sp., and uncultured Flavonifractor sp. strains were significantly increased after FMT, while Acinetobacter sp. and other strains decreased. FMT impacts amino acid metabolism and unsaturated fatty acid synthesis in Parkinson's patients. Macrogenomic and metabolomic integrated analysis showed that the changes in metabolites in fecal bacteria-transplanted offspring were highly correlated with specific gut strains.Conclusion This pilot study demonstrated that FMT was safe and may have the potential to improve constipation and neurological symptoms in patients with Parkinson’s disease.
Genome-wide identification and analysis of the WRKY gene family and low-temperature stress response in Prunus sibirica
Authors: Liu Quangang , Wang Shipeng , Wen Jiaxing , Chen Jianhua , Sun Yongqiang , Dong Shengjun 
Published: May 2023

Abstract
Background: WRKY transcription factors are a prominent gene family in plants, playing a crucial role in various biological processes including development, metabolism, defense, differentiation, and stress response. Although the WRKY gene family has been extensively studied and analysed in numerous plant species, research on Prunus sibirica's WRKYgenes (PsWRKY) remains lacking.
Results: This study analysed the basic physicochemical properties, phylogeny, gene structure, cis-acting elements, and Gene ontology (GO) annotation of PsWRKY gene family members using bioinformatics methods based on the whole-genome data of P. sibirica. In total, 55 WRKYswere identified in P. sibirica and were heterogeneously distributed on eight chromosomes. Based on the phylogenetic analysis, these WRKYs were classified into three major groups: Group I, Group II (II-a, II-b, II-c, II-d, II-e), and Group III. Members of different subfamilies have different cis-acting elements, conserved motifs, and intron-exon structures, indicating functional heterogeneity of the WRKY family. Prediction of subcellular localisation indicated that PsWRKYs were mainly located in the nucleus. Twenty pairs of duplicated genes were identified, and segmental duplication events may play an important role in PsWRKY gene family expansion. Analysis of the Ka/Ks ratio showed that the PsWRKY family's homologous genes were primarily purified by selection. Additionally, GO annotation analysis showed that the WRKY gene family was mainly involved in responses to stimuli, immune system processes, and reproductive processes. Furthermore, quantitative real-time PCR (qRT-PCR) analysis showed that 23 PsWRKYswere highly expressed in one or more tissues (pistils and roots) and PsWRKYs showed specific expression patterns under different low-temperature stress conditions.
Conclusions: Our results provide a scientific basis for the further exploration and functional validation of WRKYs in P. sibirica.
Molecular detection of Sodalis glossinidius, Spiroplasma and Wolbachia endosymbionts in wild population of tsetse flies collected in Cameroon, Chad and Nigeria
Authors: Mfopit Youssouf Mouliom , Weber Judith Sophie , Chechet Gloria Dada , Ibrahim Mahamat Alhadj Moussa , Signaboubo Djoukzoumka , Achukwi Daniel Mbunkah , Mamman Mohammed , Balogun Emmanuel Oluwadare , Shuaibu Mohammed Nasir , Kabir Junaidu , Kelm Soerge 
Published: May 2023

Abstract
Background Tsetse flies are cyclical vectors of African trypanosomiasis. They have established symbiotic associations with different bacteria, which influence certain aspects of their physiology. The vector competence of tsetse flies for different trypanosome species is highly variable and is suggested to be affected by various factors, amongst which are bacterial endosymbionts. Symbiotic interactions may provide an avenue for the disease control. The current study provided the prevalence of 3 tsetse symbionts in Glossina species from Cameroon, Chad and Nigeria.Results Tsetse flies were collected from five different locations and dissected. DNA was extracted and polymerase chain reaction PCR was used to detect the presence of Sodalis glossinidius, Spiroplasma sp and Wolbachia using specific primers. A total of 848 tsetse samples were analysed: Glossina morsitans submorsitans (47.52%), Glossina palpalis palpalis (37.26%), Glossina fuscipes fuscipes (9.08%) and Glossina tachinoides (6.13%). Only 95 (11.20%) were infected with at least one of the 3 symbionts. Among the infected, 6 (6.31%) were carrying mixed infection (Wolbachia and Spiroplasma). The overall symbiont prevalence was 0.88%, 3.66% and 11.00% respectively, for Sodalis, Spiroplasma and Wolbachia. Prevalence varied between countries and tsetse species. No Spiroplasma was detected in samples from Cameroon and no Sodalis was found in samples from Nigeria.Conclusion The present study revealed for the first time, the presence of infection by Spiroplasma in tsetse in Chad and Nigeria. These findings provide useful information to the repertoire of bacterial flora of tsetse flies and incite to more investigations to understand their implication in the vector competence of tsetse flies.
Clinical Lipid Metabolism Correlates with Urinary Calculus: TyG Index Has a Closer Correlation
Authors: Pan Yongdong , Xu Yan , Zhang Liying , Huang Yunteng , Qian Subo , Xu Guofeng 
Published: May 2023

Abstract
Aims:We aimed to assess the association between lipid metabolism and kidney stones in Chinese adults.
Methods:Data were obtained from the clinical data of our center in the past 3 years. The analysis included complete data on blood lipids and kidney stones in participants aged ≥18 years and non-stone controls. Weighted multivariate regression analysis and subgroup analysis were used to estimate the relationship between various lipid indexes and nephrolithiasis and burden and recurrence.
Results: A total of 414 participants were included in the study, and their gender, age and other basic indicators showed no significant differences. In the adjusted logistic regression analysis results, the prevalence of kidney stones increased with the increase of triglyceride (TG), triglyceride-glucose (TyG) index and Apolipoprotein A-I (AIP) [TG:
odds ratio (OR)= 2.14; 95% CI: 1.62-2.81; p < 0.001; TyG: OR=4.30; 95% CI: 2.75-6.72; p < 0.001; AIP: OR=10.12; 95% CI: 4.58-22.35; p < 0.001]. There was still a significant correlation with TyG index in the subgroup analysis of burden (OR=1.52; 95% CI: 1.04-2.22; p < 0.05) and recurrence (OR=2.77; 95% CI: 1.08-7.13; p < 0.05).
Conclusions:Lipid metabolism is closely related to urinary system stones, and a higher TyG index is associated with an increased risk of kidney stone incidence, burden, and recurrence. We hypothesize that timely treatment and management of abnormal lipid metabolism may help improve or alleviate the occurrence, burden, and recurrence of urinary system stones. However, further large-scale prospective studies and basic experiments are still needed to clarify the exact causal relationship of this association.
Assessment and Prognostic Significance of a Serum Cytokine Panel in Diffuse Large B-cell Lymphoma
Authors: xie shufang , Zhu Lifen , Wang Lei , Wang Shibing , Tong Xiangmin , Ni Wanmao 
Published: May 2023

AbstractObjectiveTo assess the contents of circulating cytokines in patients with diffuse large B-cell lymphoma (DLBCL), and to examine their relationship with clinicopathological manifestations and prognosis.MethodWe recruited 72 DLBCL patients, 11 chronic lymphocytic leukemia/small lymphocytic lymphoma (CLL/SLL) patients, and 56 healthy controls from our hospital between the period of January 2017 and January 2020, and measured 7 serum cytokine contents using Beckman Navios flow cytometry. The cytokine level was compared between DLBCL patients and healthy controls using one way ANOVA. Two-sided Spearman test was employed for relationship evaluation between circulating cytokine levels and clinicopathological characteristics, IPI score, and short-term treatment response within DLBCL patients. The inter-group comparison of cytokine levels employed the Mann Whitney test. The support vector machine (SVM) was utilized for the cytokine evaluation-based prediction of DLBCL patient short-term treatment response. Lastly, survival curves were used to assess correlation between the aforementioned cytokines and overall survival.ResultThe IL-6, IL-10, and IFN-γcontents were markedly enhanced among DLBCL patients, as opposed to healthy controls (P < 0.05). Patients with enhanced circulating LDH expressed elevated IL-10 level (P < 0.05), and patients with augmented CRP expressed upregulated IL-6 and IL-10 levels, and the rise in IL-6 levels was positively associated with serum CRP (P = 0.00,r = 0.66). Additionally, the international prognostic index (IPI) risk stratification of DLBCL patients was strongly associated with the circulating IL-6 and IL-10 contents. Enhanced IL-6, IL-10, and TNF-α levels often produced worse short-term treatment efficacies (P < 0.05). Moreover, the accuracy of short-term treatment response prediction model of DLBCL patients, obtained using SVM, was 81.63%. Using long-term follow-up, we further revealed that the DLBCL patients who expired within one year exhibited enhanced circulating IL-6 and IL-10 levels, compared to patients who survived, however, the IL-17 level was drastically reduced (P < 0.05). Despite the aforementioned evidences, we observed no marked association between the specified cytokines and overall survival (OS).ConclusionThe IL-6, IL-10, IL-17, TNF-α, and IFN-γ contents can potentially serve as biological indicators of DLBCL tumor immune status, and a combined application with the IPI score can be a robust indicator for DLBCL patient prognosis. Our findings provide novel ideas for the clinical treatment of DLBCL patients.
Investigating the antibacterial, anti-motility and anti-biofilm potential of Syzygium cumini mediated silver nanoparticles against emerging multi-drug resistant nosocomial pathogens
Authors: Diksha Diksha , Pai Manju Ohri , Gupta Pratima , Verma Sahil , Banerjee Uttam Chand , Kalita Deepjyoti 
Published: May 2023

Abstract
The current landscape of antibiotic failures promotes researchers to turn their attention to nanotechnology. Therefore, in this study silver nanoparticles (AgNPs) were synthesized by using the leaf extract of Syzygium cumini. The spectrum of biosynthesized silver nanoparticles was noticed at 432 nm displaying a bell-shaped curve under UV-Vis spectroscopy. Transmission electron microscopy (TEM) showed that these nanoparticles have a spherical shape and diameter of 50–60 nm. FT-IR study has revealed the presence of phenolics, aromatic, and alkynes groups in the synthesized nanoparticles. These biosynthesized AgNPs were processed for antibacterial,anti-motility, and anti-biofilm activity. Results showed that AgNPs exhibit good antibacterial activity. A significant reduction in motility patterns has been visualized by the effect of AgNPs against P. aeruginosa and A. baumannii. Substantial and structural reduction in P. aeruginosa and A. baumannii biofilm morphology was also examined by the effect of AgNPs. Hence greener route of AgNPs synthesis may be validated as a feasible substitute in eliminating the infection caused by multi-drug resistant nosocomial pathogens.