bluuebunny/crossref_metadata_2025_split huggingface dataset:
Metal‐Rich Metallaboranes: Synthesis, Structure, and Bonding of Heteronuclear Trimetallic Clusters containing (μ3‐BH) Ligand
Authors: Nanda Pradhan Alaka , Jaiswal Shippy , Ghosh Sundargopal 
Published: July 2023

AbstractBuilding upon our earlier results on the chemistry of nido‐1,2‐[(Cp*RuH)2B3H7] (Cp*=ɳ5‐C5Me5) (nido‐1) with different transition metal carbonyls, we continued to investigate the reactivity with group 7 metal carbonyls under photolytic condition. Photolysis of nido‐1 with [Mn2(CO)10] led to the isolation of a trimetallic [(Cp*Ru)2{Mn(CO)3}(μ‐H)(μ‐CO)3(μ3‐BH)] (2) cluster with a triply bridging borylene moiety. Cluster 2 is a rare example of a tetrahedral cluster having hydrido(hydroborylene) moiety. In an attempt to synthesize the Re analogue of 2, a similar reaction was carried out with [Re2(CO)10] that yielded the trimetallic [(Cp*Ru)2{Re(CO)3}(μ‐H)(μ‐CO)3(μ3‐BH)] (3) cluster having a triply bridging borylene unit. Along with 3, a trimetallic square pyramid cluster [(Cp*Ru)2{Re(CO)3}(μ‐H)2(μ‐CO)(μ3,ɳ2‐B2H5)] (4), and heterotrimetallic hydride clusters [{Cp*Ru(CO)2}‐{Re(CO)4}2(μ‐H)] (5) and [{Cp*Ru(CO)}{Re(CO)4}2(μ‐H)3] (6) were isolated. Cluster 4 is a unique example of a M2M′B2 cluster having diboron capped Ru2Re‐triangle. The hydride clusters 5 and 6 have triangular RuRe2 frameworks with one and three μ‐Hs respectively. All the clusters have been characterized by using mass spectrometry, 1H, 11B{1H}, 13C{1H} NMR and IR spectroscopies analyses and the structures of clusters 2–6 have been unambiguously established by XRD analyses. Furthermore, to understand the electronic, structural, and bonding features of the synthesized metal‐rich clusters, DFT calculations have been performed.
Simulation model of a non‐contact triboelectric nanogenerator based on electrostatic induction
Authors: You Jing , Shao Jiajia , He Yahua , Guo Xin , See K. W. , Wang Zhong Lin , Wang Xiaolin 
Published: July 2023

AbstractBased on the coupling effects of contact electrification and electrostatic induction, a triboelectric nanogenerator (TENG) can convert mechanical energy into electric power, which is at the cutting edge of alternative energy technology. Although a considerable number of TENGs with different configurations have been designed, some of them however, which only depend on the electrostatic induction effect have not received enough attention. Here, a non‐contact TENG model consists of copper rings and charged dielectric sphere is presented, which is aimed at exploring the working process of TENGs caused by electrostatic induction. Two classical models, including vertical and horizontal double copper rings models are also proposed. Relevant advanced and accurate models of TENGs have been established through the finite element method. We anticipate that the constructed model and theoretical analysis are helpful for the design of non‐contact model TENGs with complicated geometric construction, and expand their applications in various fields.image
“One stone two birds” design for hollow spherical Na4Fe3(PO4)2P2O7/C cathode enabled high‐performance sodium‐ion batteries from iron rust
Authors: Chen Yiqing , Dong Chongrui , Chen Long , Fu Chenglong , Zeng Yubin , Wang Qin , Cao Yuliang , Chen Zhongxue 
Published: July 2023

AbstractSodium‐ion battery (SIB) is considered as a revolutionary technology toward large‐scale energy storage applications. Developing cost‐effective cathode material as well as economical synthesis procedure is a key challenge for its commercialization. Herein, we develop a facile and economic strategy to simultaneously remove rust from the surface of carbon steel and achieve porous and hollow spherical Na4Fe3(PO4)2P2O7/C (HS‐NFPP/C). Benefiting from the desirable structure that fastens the electronic/ionic transportation and effectively accommodates the volume expansion/contraction during discharge/charge process, the as‐prepared cathode exhibits outstanding rate capability and ultralong cycle life. An extraordinarily high‐power density of 32.3 kW kg−1 with an ultrahigh capacity retention of 89.7% after 10 000 cycles are achieved. More significantly, the 3 Ah HC||HS‐NFPP/C full battery manifests impressive cycling stability. Therefore, this work provides an economical and sustainable approach for the massive production of high‐performance Na4Fe3(PO4)2P2O7 cathode, which can be potentially commercialized toward SIB applications.image
An in silico analysis of the effect of stressors on Mung bean protein
Authors: Ramalingam Saipriya , Routray Winny , Rahimi Jamshid , Kroetsch Benjamin , Singh Ashutosh 
Published: July 2023

AbstractWith the world turning its attention towards sustainable protein sources, mung bean, in recent times has garnered significant research acclaim. As an emerging functional food that is rich in protein, little is known about its characteristics during processing. Hence, in this study, an molecular dynamic (MD) simulation approach was performed on 2CV6, the crystal structure of 8Sα globulin. GROMACS software was used to vary input thermal and pressure parameters (i.e., 300, 373, and 398 K at 3, 5, and 7 Kbar). Visual MD interface was used to picture the changes occurring in the protein's secondary structure as an effect of applied stress. The radius of gyration values decreased significantly with increasing pressure while high‐pressure high‐temperature treatment improved packing effects. STRIDE analysis showed that peripheral 2° structures such as α‐helices and β‐sheets underwent conformational changes to form turns and coils, indicating increased randomness. Despite subjecting the protein molecule to high temperature, the pressure applied counteracted the unwinding process, resulting in overall compaction.
Knockout of microglial Hv1 proton channel reduces neurotoxic A1 astrocytes and neuronal damage via the ROS/STAT3 pathway after spinal cord injury
Authors: Li Ying , Xie Yi , Liu Rui , Wang Ziyue , Chen Peng , Wang Minghuan , Yu Zhiyuan , Wang Wei , Luo Xiang 
Published: July 2023

AbstractSpinal cord injury (SCI) causes severe functional deficits and neuronal damage, accompanied by intense glial activation. The voltage‐gated proton channel Hv1, selectively expressed on microglia, is associated with SCI progression. However, the effect of Hv1 on the phenotypes and functions of reactive astrocytes after SCI remains unclear. Here, we combined Hv1 knockout (Hv1−/−) mice and T10 spinal cord contusion to investigate the effects of microglial Hv1 on SCI pathophysiology and the phenotypes and functions of reactive astrocytes. After SCI, astrocytes proliferated and activated in the peri‐injury area and exhibited an A1‐dominant phenotype. Hv1 knockout reduced neurotoxic A1 astrocytes and shifted the dominant phenotype of reactive astrocytes from A1 to A2, enhancing synaptogenesis promotion, phagocytosis, and neurotrophy of astrocytes. Moreover, synaptic and axonal remodeling as well as motor recovery after SCI benefited from the improved astrocytic functions of Hv1 knockout. Furthermore, exogenous and endogenous reactive oxygen species (ROS) in astrocytes after SCI were reduced by Hv1 knockout. Our in vitro results showed that inhibition of ROS reduced the neurotoxic A1 phenotype in primary astrocytes via the STAT3 pathway. Similar to the effect of Hv1 knockout, the application of the ROS scavenger N‐acetylcysteine reduced SCI‐induced neurotoxic A1 astrocytes in vivo. Based on the in vivo and vitro results, we elucidated that microglial Hv1 knockout promotes synaptic and axonal remodeling in SCI mice by decreasing neurotoxic A1 astrocytes and increasing neuroprotective A2 astrocytes via the ROS/STAT3 pathway. Therefore, the Hv1 proton channel is a promising target for the treatment of SCI.
Clinical characteristics of COVID‐19 patients infected by the Omicron variants in Macao, China: A cross‐sectional study
Authors: Cheong Hou Hon , Sio Fong I , Chan Chi Chung , Neng Seong In , Sam Ip Pio , Cheang Teng , Tou Weng Ieong , Lei Hong San , Cheong Tan Fong , Lao Edmundo Patricio Lopes , Cheong Tak Hong , Kuok Cheong U , Lo Iek Long 
Published: July 2023

AbstractBackground and AimsThe evolving mutants of SARS‐CoV‐2 have made the COVID‐19 pandemic sustained for over 3 years. In 2022, BA.4 and BA.5 were the Omicron variants dominating the spread globally. Although COVID‐19 was no longer a Public Health Emergency of International Concern (PHEIC) as announced by WHO, the SARS‐CoV‐2 variants remain a challenge to global healthcare under the circumstances of withdrawal and loosening of personal protective behavior in the post‐quarantine era. This study aims to acknowledge the clinical characteristics caused by Omicron BA.4/BA.5 in COVID‐19 naive people and analyze possible factors affecting disease severities.MethodsIn this retrospective study, we report and analyze the clinical features of 1820 COVID‐19 patients infected with the BA.4/BA.5 Omicron variants of SARS‐CoV‐2 during a local outbreak that occurred in Macao SAR, China, from June to July 2022.ResultsA total of 83.5% of patients were symptomatic eventually. The most common symptoms were fever, cough, and sore throat. Hypertension, dyslipidemia, and diabetes mellitus were the leading comorbidities. There were significantly more elderly patients (p < 0.001), more patients with comorbidity (p < 0.001) and more patients without vaccination or not completing the series (p < 0.001) in the “Severe to Critical” group. All deceased patients were elderly with at least three comorbidities and were partial to totally dependent in their daily lives.ConclusionOur data are consistent with a milder disease caused by BA.4/5 Omicron variants in the general population, while patients with old age and comorbidities have developed severe to critical diseases. Complete vaccination series and booster doses are effective strategies to reinforce protection against severe diseases and avoid mortality.
Detection and classification of hepatocytes and hepatoma cells using atomic force microscopy and machine learning algorithms
Authors: Zeng Yi , Liu Xianping , Wang Zuobin , Gao Wei , Li Li , Zhang Shengli 
Published: July 2023

AbstractHepatocellular carcinoma is a high‐risk malignant tumor. Hepatoma cells are transformed from normal cells and have unique surface nanofeatures in addition to the characteristics of the original cells. In this paper, atomic force microscopy was used to extract the three‐dimensional morphology and mechanical information of HL‐7702 human hepatocytes and SMMC‐7721 and HepG2 hepatoma cells in culture, such as the elastic modulus and viscoelasticity. The characteristics of different cells were compared and analyzed. Finally, the cell morphology and mechanics information were used for training machine learning algorithms. With the trained model, the detection of cells was realized. The classification accuracy was as high as 94.54%, and the area under the receiver operating characteristic (ROC) curve (AUC) was 0.99. Thus, hepatocytes and hepatoma cells were accurately identified and assessed. We also compared the classification effects of other machine learning algorithms, such as support vector machine and logistic regression. Our method extracts cellular nanofeatures directly from the surface of cells of unknown type for cell classification. Compared with microscope image‐based analysis and other methods, this approach can avoid the misjudgment that may occur when different doctors have different levels of experience. Thus, the proposed method provides an objective basis for the early diagnosis of hepatocellular carcinoma.Research Highlights
The 3D appearance and mechanical characteristics of hepatocellular carcinoma cells are very similar to those of hepatocytes.
Application of atomic force microscopy with machine learning algorithm.
Collect the data set of nano‐characteristic parameters of the cell.
The machine learning algorithms is trained by data set, and its classification effect is better than that of a single nano‐parameter.
Spatiotemporal walking gait kinematics of semi‐arboreal red pandas (Ailurus fulgens)
Authors: Plocek Maura R. , Dunham Noah T. 
Published: July 2023

AbstractSemi‐arboreal mammals must routinely cope with the differing biomechanical challenges of terrestrial versus arboreal locomotion; however, it is not clear to what extent semi‐arboreal mammals adjust footfall patterns when moving on different substrates. We opportunistically filmed quadrupedal locomotion (n = 132 walking strides) of semi‐arboreal red pandas (Ailurus fulgens;n = 3) housed at Cleveland Metroparks Zoo and examined the effects of substrate type on spatiotemporal gait kinematic variables using linear mixed models. We further investigated the effects of substrate diameter and orientation on arboreal gait kinematics. Red pandas exclusively used lateral sequence (LS) gaits and most frequently utilized LS lateral couplet gaits across terrestrial and arboreal substrates. Red pandas moved significantly slower (p < 0.001), and controlling for speed, had significantly greater relative stride length (p < 0.001), mean stride duration (p = 0.002), mean duty factor (p < 0.001), and mean number of supporting limbs (p < 0.001) during arboreal locomotion. Arboreal strides on inclined substrates were characterized by significantly faster relative speeds and increased limb phase values compared with those horizontal and declined substrates. These kinematics adjustments help to reduce substrate oscillations thereby promoting stability on potentially precarious arboreal substrates. Red panda limb phase values are similar to those of (primarily terrestrial) Carnivora examined to date. Despite the similarity in footfall patterns during arboreal and terrestrial locomotion, flexibility in other kinematic variables is important for semi‐arboreal red pandas that must navigate disparate biomechanical challenges inherent to arboreal versus terrestrial locomotion.
Identification of module genes and functional pathway analysis in septic shock subtypes by integrated bioinformatics analysis
Authors: Mao Yujing , Lei Ruyi , Pei Hui , Zhang Yepeng , Jiang Yumin , Gu Yulei , Zhu Changjv , Zhu Zhiqiang 
Published: July 2023

AbstractBackgroundThe present study aimed to identify the module genes and key gene functions and biological pathways of septic shock (SS) through integrated bioinformatics analysis.MethodsIn the study, we performed batch correction and principal component analysis on 282 SS samples and 79 normal control samples in three datasets, GSE26440, GSE95233 and GSE57065, to obtain a combined corrected gene expression matrix containing 21,654 transcripts. Patients with SS were then divided into three molecular subtypes according to sample subtyping analysis.ResultsBy analyzing the demographic characteristics of the different subtypes, we found no statistically significant differences in gender ratio and age composition among the three groups. Then, three subtypes of differentially expressed genes (DEGs) and specific upregulated DEGs (SDEGs) were identified by differential gene expression analysis. We found 7361 DEGs in the type I group, 5594 DEGs in the type II group, and 7159 DEGs in the type III group. There were 1698 SDEGs in the type I group, 2443 in the type II group, and 1831 in the type III group. In addition, we analyzed the correlation between the expression data of 5972 SDEGs in the three subtypes and the gender and age of 227 patients, constructed a weighted gene co‐expression network, and identified 11 gene modules, among which the module with the highest correlation with gender ratio was MEgrey. The modules with the highest correlation with age composition were MEgrey60 and MElightyellow. Then, by analyzing the differences in module genes among different subgroups of SS, we obtained the differential expression of 11 module genes in four groups: type I, type II, type III and the control group. Finally, we analyzed the Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment of all module DEGs, and the GO function and KEGG pathway enrichment of different module genes were different.ConclusionsOur findings aim to identify the specific genes and intrinsic molecular functional pathways of SS subtypes, as well as further explore the genetic and molecular pathophysiological mechanisms of SS.
Clinical presentation of 13 children with alkaptonuria
Authors: Kujawa Mariusz J. , Świętoń Dominik , Wierzba Jolanta , Grzywińska Małgorzata , Budziło Oskar , Limanówka Monika , Pierzynowska Karolina , Gaffke Lidia , Grabowski Łukasz , Cyske Zuzanna , Rintz Estera , Rąbalski Łukasz , Kosiński Maciej , Węgrzyn Grzegorz , Mański Arkadiusz , Anikiej‐Wiczenbach Paulina , Ranganath Lakshminarayan , Piskunowicz Maciej 
Published: July 2023

AbstractUntil now, only a few studies have focused on the early onset of symptoms of alkaptonuria (AKU) in the pediatric population. This prospective, longitudinal study is a comprehensive approach to the assessment of children with recognized AKU during childhood. The study includes data from 32 visits of 13 patients (five males, eight females; age 4–17 years) with AKU. A clinical evaluation was performed with particular attention to eye, ear, and skin pigmentation, musculoskeletal complaints, magnetic resonance imaging (MRI), and ultrasound (US) imaging abnormalities. The cognitive functioning and adaptive abilities were examined. Molecular genetic analyses were performed. The most common symptoms observed were dark urine (13/13), followed by joint pain (6/13), and dark ear wax (6/13). In 4 of 13 patients the values obtained in the KOOS‐child questionnaire were below the reference values. MRI and US did not show degenerative changes in knee cartilages. One child had nephrolithiasis. Almost half of the children with AKU (5/13) presented deficits in cognitive functioning and/or adaptive abilities. The most frequent HGD variants observed in the patients were c.481G>A (p.Gly161Arg) mutation and the c.240A>T (p.His80Gln) polymorphism. The newly described allele of the HGD gene (c.948G>T, p.Val316Phe) which is potentially pathogenic was identified.
The impact of liver transplantation on health‐related quality of life in (acute) intoxication‐type inborn errors of metabolism
Authors: Greco Benedetta , Caviglia Stefania , Martinelli Diego , Capitello Teresa Grimaldi , Liccardo Daniela , De Nictolis Francesca , Pietrobattista Andrea , Huemer Martina , Piga Simone , Olivieri Giorgia , Spagnoletti Gionata , Spada Marco , Dionisi‐Vici Carlo 
Published: July 2023

AbstractOrganic acidurias (OAs), urea‐cycle disorders (UCDs), and maple syrup urine disease (MSUD) belong to the category of intoxication‐type inborn errors of metabolism (IT‐IEM). Liver transplantation (LTx) is increasingly utilized in IT‐IEM. However, its impact has been mainly focused on clinical outcome measures and rarely on health‐related quality of life (HRQoL). Aim of the study was to investigate the impact of LTx on HrQoL in IT‐IEMs. This single center prospective study involved 32 patients (15 OA, 11 UCD, 6 MSUD; median age at LTx 3.0 years, range 0.8–26.0). HRQoL was assessed pre/post transplantation by PedsQL‐General Module 4.0 and by MetabQoL 1.0, a specifically designed tool for IT‐IEM. PedsQL highlighted significant post‐LTx improvements in total and physical functioning in both patients' and parents' scores. According to age at transplantation (≤3 vs. >3 years), younger patients showed higher post‐LTx scores on Physical (p = 0.03), Social (p < 0.001), and Total (p =0.007) functioning. MetabQoL confirmed significant post‐LTx changes in Total and Physical functioning in both patients and parents scores (p ≤ 0.009). Differently from PedsQL, MetabQoL Mental (patients p = 0.013, parents p = 0.03) and Social scores (patients p = 0.02, parents p = 0.012) were significantly higher post‐LTx. Significant improvements (p = 0.001–0.04) were also detected both in self‐ and proxy‐reports for almost all MetabQoL subscales. This study shows the importance of assessing the impact of transplantation on HrQoL, a meaningful outcome reflecting patients' wellbeing. LTx is associated with significant improvements of HrQol in both self‐ and parent‐reports. The comparison between PedsQL‐GM and MetabQoL highlighted that MetabQoL demonstrated higher sensitivity in the assessment of disease‐specific domains than the generic PedsQL tool.
循環動態不安定な小児外傷性肝損傷に対し防災ヘリ搬送のため約1時間のIABO inflationを行った1救命例(Survival of a hemodynamically unstable pediatric patient with liver trauma who underwent 1h aortic balloon occlusion for air transport)
Authors: 雄一 三宅 , 裕子 置塩 , 尚明 柴田 , 秀治 川嶋 , 亨 那須 , 健太郎 上田 , 正哉 加藤 
Published: July 2023

要旨　近年，体幹部外傷による出血性ショックに対してIABOが広く利用されている。出血性ショックの成人外傷患者にIABOを用いて長距離搬送が行われた報告はあるが，小児患者での報告は限られる。本症例は，11歳女児が軽乗用車にはねられ受傷し，当院から約60km離れた前医へ搬送された。搬送時よりショック状態で，造影CTで肝損傷を認め，止血処置目的に当院へヘリ搬送の方針となった。前医でIABOが留置され，バルーン遮断された状態で搬送された。当院搬送後に開腹止血術と経カテーテル動脈塞栓術が施行された。術後は一時的な肝腎機能悪化が見られたが，その後は改善し，近隣病院へ転院後に自宅退院した。重症腹部外傷の小児患者に対して，IABOを用いた転院搬送を行うことで救命へとつなげることができた。また，小児での報告例は限られており，エビデンス構築のためにも今後さらなる議論が必要である。
Isolation and characterization of emerging Mpox virus from India
Authors: Sharma Sushil Kumar , Dash Paban Kumar , Yadav Ram Govind , Shrivastava Ambuj , Menon Rohit , Kumar Jyoti S. , Sharma Shashi , Dhankher Suman , Dhiman Sunil , Kumari Divya , Shukla Manisha , Relhan Vineet , Kumar Suresh , Parida Manmohan 
Published: July 2023

AbstractMpox (previously known as Monkeypox) has recently re‐emerged, primarily through human‐to‐human transmission in non‐endemic countries including India. Virus isolation is still considered as the gold standard for diagnosis of viral infections. Here, the qPCR positive skin lesion sample from a patient was inoculated in Vero E6 cell monolayer. Characteristic cytopathic effect exhibiting typical cell rounding and detachment was observed at passage‐02. The virus isolation was confirmed by qPCR. The replication kinetics of the isolate was determined that revealed maximum viral titre of log 6.3 PFU/mL at 72 h postinfection. Further, whole genome analysis through next generation sequencing revealed that the Mpox virus (MPXV) isolate is characterized by several unique SNPs and INDELs. Phylogenetically, it belonged to A.2 lineage of clade IIb, forming a close group with all other Indian MPXV along with few from USA, UK, Portugal, Thailand and Nigeria. This study reports the first successful isolation and phenotypic and genotypic characterization of MPXV from India.
Femtosecond Laser Fabrication of Refractive/Diffractive Micro‐Optical Components on Hard Brittle Materials
Authors: Tan Jia‐Wei , Wang Gong , Li Yunfei , Yu Yu , Chen Qi‐Dai 
Published: July 2023

AbstractMicro‐optical components exhibit significant potential applications in various fields such as imaging, optical fiber communication, and aerospace owing to their small size, light weight, and flexible design. It is imperative to fabricate micro‐optical components on hard brittle materials to improve stability and broaden the optical transmission range. Therefore, femtosecond laser processing technology is demonstrated to be an efficient processing strategy for preparing refractive/diffractive micro‐optical components on hard brittle materials compared to other established micromachining techniques. This study reviews recent advances in refractive/diffractive micro‐optical components using femtosecond laser processing technologies. The applications of refractive/diffractive micro‐optical components with high accuracy, efficiency, and quality in the fields of imaging, sensing, display, and integration are also summarized. Moreover, the challenges of femtosecond laser processing technologies and the outlook of micro‐optical components are highlighted.