bluuebunny/crossref_metadata_2025_split huggingface dataset:
Pre-pregnancy body mass index has greater influence on newborn weight and perinatal outcome than weight control during pregnancy in obese women
Authors: Zhang Jin , Zhang Rui , Chi Jingjing , Li Ya , Bai Wenpei 
Published: January 2023

Abstract
Background
The pre-pregnancy weight and gestational weight gain (GWG) are closely related to perinatal maternal and infant complications, but the relationship between pre-pregnancy weight and GWG and the pattern of interaction have not been reported. This study investigated the influence of weight control during pregnancy on the perinatal maternal and infant outcomes.

Methods
A total of 835 singleton pregnant women who were hospitalized between January 2018 and December 2018 were retrospectively included in this study and divided into two groups: the diet guidance (DG) group (n = 167) and the control group (n = 668). The pre-pregnancy body mass index (BMI), GWG, and perinatal outcomes of the women and infants were determined in two groups.

Results
The dietary modification and reasonable exercise during pregnancy effectively reduced the GWG, and even some women with pre-pregnancy obesity achieved weight loss during pregnancy. The GWG in the DG group was significantly lower than in the control group, especially in the second and third trimesters. GWG was positively related to birth weight. The birth weight in the DG group was significantly lower than in the control group when their mothers had similar GWG. In women with pre-pregnancy obesity, GWG seemed to be negatively related to birth weight. However, after adjusting the mean BMI, the pre-pregnancy BMI and GWG were positively related to the birth weight. Compared with the control group, the incidences of dystocia, postpartum hemorrhage, macrosomia, small for gestational age infants and neonatal complications significantly reduced in the DG group, and the preterm birth rate was comparable between two groups. Some women with pre-pregnancy obesity lose weight during pregnancy, but there was no premature birth or small for gestational age infant. The incidences of macrosomia, postpartum hemorrhage, dystocia, cesarean section and gestational diabetes increased significantly with the increase of pre-pregnancy BMI.

Conclusion
For women with increased pre-pregnancy BMI, strict weight control is required to reduce obesity-related complications of the mother and infant. The weight control in the second and third trimesters is especially important and most likely to prevent GWG. Compared with GWG, pre-pregnancy BMI has greater influence on the birth weight and maternal and infant complications, and may even compromise the benefits of weight control during pregnancy. Thus, weight control is recommended before pregnancy.
Cherry fruit anthocyanins cyanidin-3-O-glucoside and cyanidin-3-O-rutinoside protect against blue light-induced cytotoxicity in HaCaT cells
Authors: Lee Hyang-Yeol , Kim Jun-Sub 
Published: January 2023

AbstractBlue light derived from multiple sources, including sunlight, generates reactive oxygen species (ROS) and negatively affects the skin in a manner similar to that of ultraviolet light. Cyanidin-3-O-glucoside (C3OG) and cyanidin-3-O-rutinoside (C3OR) are anthocyanin antioxidants that have protective effects on various tissues and cell types. However, the effects of anthocyanins on blue light-mediated changes remain unconfirmed. In this study, we determined the protective effects of C3OG and C3OR isolated and purified from waste cherry fruits (Prunus serrulata L. var. tomentella Nakai) against the blue light-induced ROS formation and inflammatory responses in HaCaT cells. It is showed that the treatment of C3OG and C3OR significantly reduced the blue light-induced cytotoxicity and ROS production in a dose dependent manner. Furthermore, we found that focal adhesion kinase (FAK) is a major upstream of blue light-induced expression of inflammatory cytokines (TNF-α, IL-6 and IL-8), and these effects were attenuated by C3OG or C3OR treatment. In the initial reaction, blue lights increased the phosphorylation of inhibitory-κB Kinase α (IKKα), c-jun N-terminal kinase (JNK), and p38. The phosphorylation of these intracellular proteins was reduced via FAK inhibitor, NAC (ROS scavenger), and anthocyanin treatments. After 24 h of blue light irradiation, C3OG or C3OR treatment markedly inhibited caspase-3-mediated apoptosis and cleaved-FAK-mediated anoikis, which is cell detachment-induced apoptosis. Therefore, our results indicate that C3OG and C3OR effectively protected human keratinocytes from harmful blue light-induced cytotoxicity and inflammation.
Dynamics of the gut microbiome, IgA response, and plasma metabolome in the development of pediatric celiac disease
Authors: Girdhar Khyati , Dogru Yusuf Dogus , Huang Qian , Yang Yi , Tolstikov Vladimir , Raisingani Amol , Chrudinova Martina , Oh Jaewon , Kelley Kristina , Ludvigsson Jonas F. , Kiebish Michael A. , Palm Noah W. , Ludvigsson Johnny , Altindis Emrah 
Published: January 2023

AbstractBackgroundCeliac disease (CD) is an autoimmune disorder triggered by gluten consumption. Almost all CD patients possess human leukocyte antigen (HLA) DQ2/DQ8 haplotypes; however, only a small subset of individuals carrying these alleles develop CD, indicating the role of environmental factors in CD pathogenesis. The main objective of this study was to determine the contributory role of gut microbiota and microbial metabolites in CD onset. To this end, we obtained fecal samples from a prospective cohort study (ABIS) at ages 2.5 and 5 years. Samples were collected from children who developed CD after the final sample collection (CD progressors) and healthy children matched by age, HLA genotype, breastfeeding duration, and gluten-exposure time (n=15–16). We first used 16S sequencing and immunoglobulin-A sequencing (IgA-seq) using fecal samples obtained from the same children (i) 16 controls and 15 CD progressors at age 2.5 and (ii) 13 controls and 9 CD progressors at age 5. We completed the cytokine profiling, and plasma metabolomics using plasma samples obtained at age 5 (n=7–9). We also determined the effects of one microbiota-derived metabolite, taurodeoxycholic acid (TDCA), on the small intestines and immune cell composition in vivo.ResultsCD progressors have a distinct gut microbiota composition, an increased IgA response, and unique IgA targets compared to healthy subjects. Notably, 26 plasma metabolites, five cytokines, and one chemokine were significantly altered in CD progressors at age 5. Among 26 metabolites, we identified a 2-fold increase in TDCA. TDCA treatment alone caused villous atrophy, increased CD4+ T cells, Natural Killer cells, and two important immunoregulatory proteins, Qa-1 and NKG2D expression on T cells while decreasing T-regulatory cells in intraepithelial lymphocytes (IELs) in C57BL/6J mice.ConclusionsPediatric CD progressors have a distinct gut microbiota composition, plasma metabolome, and cytokine profile before diagnosis. Furthermore, CD progressors have more IgA-coated bacteria and unique targets of IgA in their gut microbiota. TDCA feeding alone stimulates an inflammatory immune response in the small intestines of C57BJ/6 mice and causes villous atrophy, the hallmark of CD. Thus, a microbiota-derived metabolite, TDCA, enriched in CD progressors’ plasma, has the potential to drive inflammation in the small intestines and enhance CD pathogenesis.
The association of genetic variation in CACNA1C with resting-state functional connectivity in youth bipolar disorder
Authors: Jiang Xinyue , Sultan Alysha A. , Dimick Mikaela K. , Zai Clement C. , Kennedy James L. , MacIntosh Bradley J. , Goldstein Benjamin I. 
Published: January 2023

Abstract
Background
CACNA1C rs1006737 A allele, identified as a genetic risk variant for bipolar disorder (BD), is associated with anomalous functional connectivity in adults with and without BD. Studies have yet to investigate the association of CACNA1C rs1006737 with resting-state functional connectivity (rsFC) in youth BD.

Methods
Participants included 139 youth with BD-I, -II, or -not otherwise specified, ages 13–20 years, including 27 BD A-carriers, 41 BD non-carriers, 32 healthy controls (HC) A-carriers, and 39 HC non-carriers. Anterior cingulate cortex (ACC), amygdala, and orbitofrontal cortex (OFC) were examined as regions-of-interest in seed-to-voxel analyses. General linear models included main effects of diagnosis and rs1006737, and an interaction term, controlling for age, sex, and race.

Results
We observed a main effect of BD diagnosis on rsFC between the right amygdala and the right occipital pole (p = 0.02), and a main effect of rs1006737 genotypes on rsFC between the right OFC and bilateral occipital cortex (p < 0.001). Two significant BD diagnosis-by-CACNA1C rs1006737 interactions were also identified. The A allele was associated with positive rsFC between the right ACC and right amygdala in BD but negative rsFC in HC (p = 0.01), and negative rsFC between the left OFC and left putamen in BD but positive rsFC in HC (p = 0.01).

Conclusion
This study found that the rs1006737 A allele, identified as a genetic risk variant for BD in adults, was differentially associated with rsFC in youth with BD in regions relevant to emotion, executive function, and reward. Future task-based approaches are warranted to better understand brain connectivity in relation to CACNA1C in BD.
Implementing ICT in classroom practice: what else matters besides the ICT infrastructure?
Authors: Lomos Catalina , Luyten J. W. , Tieck Sabine 
Published: January 2023

Abstract
Background
The large-scale International Computer and Information Literacy Study (2018) has an interesting finding concerning Luxembourg teachers. Luxembourg has one of the highest reported level of technology-related resources for teaching and learning, but a relatively lower reported use of ICT in classroom practice.

Methods
ICT innovation requires a high initial level of financial investment in technology, and Luxembourg has achieved this since 2015. Once the necessary financial investment in ICT technology has been made, the key question is what else matters to increase the use of ICT in teaching. To identify the relevant factors, we used the “Four in Balance” model, aimed explicitly at monitoring the implementation of ICT in schools.

Results
Using data for 420 teachers in Luxembourg, we identify that within such a technology-driven approach to digitalization, teachers’ vision of ICT use in teaching, level of expertise, and the use of digital learning materials in class are significant support factors. Leadership and collaboration, in the form of an explicit vision of setting ICT as a priority for teaching in the school, also prove to be important.

Conclusions
Through these findings, we show that the initial investment in school infrastructure for ICT needs to be associated in its implementation with teachers’ ICT-related beliefs, attitudes, and ICT expertise.
Significance of the high-pressure properties and structural evolution of gas hydrates for inferring the interior of icy bodies
Authors: Hirai Hisako , Kadobayashi Hirokazu 
Published: January 2023

AbstractHydrogen, methane, and water ice are among the most abundant materials in the universe. Based on experimental, theoretical, and spacecraft data, gas hydrates consisting of gas and water ice have been predicted to exist throughout the universe. This review discusses the high-pressure properties of two common gas hydrates (methane and hydrogen hydrates) at low and high temperatures based primarily on experimental results. Gas hydrates consist of a water molecule host and a gaseous guest. They have a clathrate structure at low pressure and a filled-ice structure at high pressure. The host encloses the guest, and a specific interaction occurs between the guest and host, resulting in unique physical properties. When subjected to pressure, gas hydrates undergo various phase changes. Based on pressure and guest size, a general rule for phase changes occurring in gas hydrates exists. Analysis of the phase-transition mechanism shows that some cages are maintained after the transition to the next clathrate structure, while others are recombined into different cages of the next structure. This is a novel mechanism that can be called “cage recombination mechanism.” Low-temperature and high-pressure experiments have revealed that as the pressure increases, the guest molecules undergo a stepwise progression of orientational ordering, i.e., restriction of free rotation, which induces structural changes that stabilize the structure at high pressure. Theoretical studies have predicted that hydrogen-bond symmetrization in the host occurs at even higher pressures, further stabilizing the structure. Thus, hydrates respond to environmental changes such as pressure to achieve self-organization by the orientational ordering of the guest and hydrogen-bond symmetrization of the host. Additionally, results of high-temperature and high-pressure experiments conducted at conditions comparable to those in Neptune’s ice mantle show that methane hydrate decomposes into solid methane and ice VII, both of which melt at further elevated temperatures. Then, the methane molecules undergo further molecular dissociation to form diamonds. These findings are valuable for modeling the interiors of icy planets and understanding how magnetic fields and heat are generated.
Phylogenomics of five Pseudanabaena cyanophages and evolutionary traces of horizontal gene transfer
Authors: Zhu Jie , Yang Feng , Du Kang , Wei Zi-Lu , Wu Qing-Fa , Chen Yuxing , Li Wei-Fang , Li Qiong , Zhou Cong-Zhao 
Published: January 2023

AbstractBackgroundAlong with the fast development and urbanization in developing countries, the waterbodies aside the growing cities become heavily polluted and highly eutrophic, thus leading to the seasonal outbreak of cyanobacterial bloom. Systematic isolation and characterization of freshwater cyanophages might provide a biological solution to control the awful blooms. However, genomic sequences and related investigations on the freshwater cyanophages remain very limited to date.ResultsFollowing our recently reported five cyanophages Pam1~Pam5 from Lake Chaohu in China, here we isolated another five cyanophages, termed Pan1~Pan5, which infect the cyanobacteriumPseudanabaenasp. Chao 1811. Whole-genome sequencing showed that they all contain a double-stranded DNA genome of 37.2 to 72.0 kb in length, with less than half of the putative open reading frames annotated with known functions. Remarkably, the siphophage Pan1 encodes an auxiliary metabolic genephoHand constitutes, together with the host, a complete queuosine modification pathway. Proteomic analyses revealed that although Pan1~Pan5 are distinct from each other in evolution, Pan1 and Pan3 are somewhat similar to our previously identified cyanophages Pam3 and Pam1 at the genomic level, respectively. Moreover, phylogenetic analyses suggested that Pan1 resembles theα-proteobacterial phage vB_DshS-R5C, revealing direct evidence for phage-mediated horizontal gene transfer between cyanobacteria andα-proteobacteria.ConclusionIn addition to the previous reports of Pam1~Pam5, the present findings on Pan1~Pan5 largely enrich the library of reference freshwater cyanophages. The abundant genomic information provides a pool to identify novel genes and proteins of unknown function. Moreover, we found for the first time the evolutionary traces in the cyanophage that horizontal gene transfer might occur at the level of not only inter-species, but even inter-phylum. It indicates that the bacteriophage or cyanophage could be developed as a powerful tool for gene manipulation among various species or phyla.
Feasibility of electroacupuncture at Baihui (GV20) and Zusanli (ST36) on survival with a favorable neurological outcome in patients with postcardiac arrest syndrome after in-hospital cardiac arrest: study protocol for a pilot randomized controlled trial
Authors: Zeng Ruifeng , Lai Fang , Huang Manhua , Zhu Decai , Chen Baijian , Tao Lanting , Huang Wei , Lai Chengzhi , Ding Banghan 
Published: January 2023

Abstract
Background
At present, even the first-line medication epinephrine still shows no evidence of a favourable neurological outcome in patients with sudden cardiac arrest (SCA). The high mortality of patients with postcardiac arrest syndrome (PCAS) can be attributed to brain injury, myocardial dysfunction, systemic ischaemia/reperfusion response, and persistent precipitating pathology. Targeted temperature management, the only clinically proven method in the treatment of PCAS, is still associated with a series of problems that have not been completely resolved.
Acupuncture is a crucial therapy in traditional Chinese medicine. On the basis of the results of previous studies, we hypothesize that electroacupuncture (EA) might provide therapeutic benefits in the treatment of PCAS. This study will explore the feasibility of EA on SCA patients.

Methods
This is a prospective pilot, randomized controlled clinical trial. Eligible patients with PCAS after in-hospital cardiac arrest (IHCA) admitted to our department will be randomly allocated to the control group or the EA group. Both groups will receive standard therapy according to American Heart Association guidelines for cardiopulmonary resuscitation. However, the EA group will also receive acupuncture at the Baihui acupoint (GV20) and Zusanli acupoint (ST36) with EA stimulation for 30 min using a dense-dispersed wave at frequencies of 20 and 100 Hz, a current intensity of less than 10 mA, and a pulse width of 0.5 ms. EA treatment will be administered for up to 14 days (until either discharge or death). The primary endpoint is survival with a favourable neurological outcome. The secondary endpoints are neurological scores, cardiac function parameters, and other clinical parameters, including Sequential Organ Failure Assessment (SOFA) scores and Acute Physiology and Chronic Health Evaluation (APACHE) II scores, on days 0 to 28.

Discussion
This study will provide crucial clinical evidence on the efficacy of EA in PCAS when used as an adjunctive treatment with AHA standard therapy.

Trial registration
chictr.org.cn: ChiCTR2000040040. Registered on 19 November 2020. Retrospectively registered. http://www.chictr.org.cn/.
Upregulation of CENPM is associated with poor clinical outcome and suppression of immune profile in clear cell renal cell carcinoma
Authors: Zhang Zhi-Cheng , Liu Yi-Fu , Xi Ping , Nie Ye-Chen , Sun Ting , Gong Bin-Bin 
Published: January 2023

Abstract
Background
The response of advanced clear cell renal cell carcinoma (ccRCC) to immunotherapy is still not durable, suggesting that the immune landscape of ccRCC still needs to be refined, especially as some molecules that have synergistic effects with immune checkpoint genes need to be explored.

Methods
The expression levels of CENPM and its relationship with clinicopathological features were explored using the ccRCC dataset from TCGA and GEO databases. Quantitative polymerase chain reaction (qPCR) analysis was performed to validate the expression of CENPM in renal cancer cell lines. Kaplan-Meier analysis, COX regression analysis and Nomogram construction were used to systematically evaluate the prognostic potential of CENPM in ccRCC. Besides, single gene correlation analysis, protein–protein interaction (PPI) network, genetic ontology (GO), kyoto encyclopedia of genes and genomes (KEGG) and gene set enrichment analysis (GSEA) were used to predict the biological behaviour of CENPM and the possible signalling pathways involved. Finally, a comprehensive analysis of the crosstalk between CENPM and immune features in the tumor microenvironment was performed based on the ssGSEA algorithm, the tumor immune dysfunction and exclusion (TIDE) algorithm, the TIMER2.0 database and the TISIDB database.

Results
CENPM was significantly upregulated in ccRCC tissues and renal cancer cell lines and was closely associated with poor clinicopathological features and prognosis. Pathway enrichment analysis revealed that CENPM may be involved in the regulation of the cell cycle in ccRCC and may have some crosstalk with the immune microenvironment in tumors. The ssGSEA algorithm, CIBERSOPT algorithm suggests that CENPM is associated with suppressor immune cells in ccRCC such as regulatory T cells. The ssGSEA algorithm, CIBERSOPT algorithm suggests that CENPM is associated with suppressor immune cells in ccRCC such as regulatory T cells. Furthermore, the TISIDB database provides evidence that not only CENPM is positively associated with immune checkpoint genes such as CTLA4, PDCD1, LAG3, TIGIT, but also chemokines and receptors (such as CCL5, CXCL13, CXCR3, CXCR5) may be responsible for the malignant phenotype of CENPM in ccRCC. Meanwhile, predictions based on the TIDE algorithm support that patients with high CENPM expression have a worse response to immunotherapy.

Conclusions
The upregulation of CENPM in ccRCC predicts a poor clinical outcome, and this malignant phenotype may be associated with its exacerbation of the immunosuppressive state in the tumor microenvironment.
Detection and characterization of difenoconazole resistance in Stagonosporopsis citrulli from watermelon and muskmelon in Zhejiang Province of China
Authors: Kong Zhangliang , Zhang Yu , Zhuang Chaojie , Mao Chenxin , Zhang Chuanqing 
Published: January 2023

AbstractThe watermelon and muskmelon productions are important agriculture pillar industries of Zhejiang Province in China. Difenoconazole is an imidazole-class sterol, 14-demethylase inhibitor (DMI), which has been in use for several years to control gummy stem blight (GSB) caused by Stagonosporopsis species. However, the detection and characterization of difenoconazole resistance in GSB have not been clarified. In this study, a total of 112 isolates were collected from samples of GSB on watermelon and muskmelon in five locations of Zhejiang Province, China. All of the isolates were identified as Stagonosporopsis citrulli through morphology and multiplex PCR analysis. The determination of their resistance to difenoconazole via the discriminatory dosage method showed that the total resistance frequency was 89.3%. Among the resistant sub-population, 36.6% had high-level resistance to difenoconazole (DifHR), while 46.4% and 6.3% had low- (DifLR) and moderate-level resistance (DifMR), respectively. Additionally, the difenoconazole showed a positive cross-resistance with four DMIs, i.e., tebuconazole, prochloraz, metconazole, and mefentrifluconazole, but not hexaconazole. The phenotypic analysis found that the difenoconazole resistant (DifR) isolates demonstrated attenuated ability in both the mycelial growth and sporulation compared with the difenoconazole sensitive (DifS) isolates, while there was no significant difference in pathogenicity on watermelon leaves between the DifR and DifS isolates. Further exploration of the mechanism related to difenoconazole resistance of S. citrulli isolates revealed that the resistance to difenoconazole involved four types of mutations in CYP51, i.e., G463S for DifLR, I444M, Y446H, and A464G for DifHR. No over-expression of the cyp51 gene was found in the tested DifR isolates. Furthermore, it was found that 5% of the DifR isolates were significantly more sensitive to difenoconazole after being treated with 20 μg/mL chlorpromazine hydrochloride, indicating that the efflux mechanism may be involved in these difenoconazole-resistant isolates. Together, our study results suggested that S. citrulli had a strong resistance to difenoconazole on watermelon and muskmelon, and the mutations in cyp51and changes in fungicide efflux were responsible for the emergence of difenoconazole resistance in S. citrulli.