bluuebunny/crossref_metadata_2025_split huggingface dataset:
An Indolin‐3‐imine Photobase and pH Sensitive Fluorophore
Authors: Doloczki Susanne , Kern Christoph , Holmberg Karl O. , Zhao Miao , Swartling Fredrik J. , Streuff Jan , Dyrager Christine 
Published: September 2023

AbstractThis work presents the pH sensing ability of a fluorescent indolin‐3‐imine derivative. Protonation of the weakly basic imine (pKa=8.3 of its conjugate acid) results in a significant red‐shift of the absorption band. The fluorophore acts as a photobase, with a basicity increase of approximately 6 units upon photoexcitation. This behavior promotes excited state proton transfer from weak acids such as protic solvents. The characteristics of the fluorophore enable sensing of water fractions in organic solvents and differentiation between methanol, ethanol, and longer chain alcohols. Initial cell studies indicated the future potential of indolin‐3‐imines as fluorophores for bioimaging applications.
CuBO2: A Potential Alternative for NiO as a Hole Acceptor Layer
Authors: Zhu Kaijian , Mul Guido , Huijser Annemarie 
Published: September 2023

AbstractP‐type metal oxides, and in particular NiO, are typically used as hole accepting layers in dye‐sensitized photocathodes. Delafossites (CuMO2) with M=B, Al, Cr or Ga have recently been proposed as attractive substitutes for NiO, with theoretically a higher hole mobility than NiO, therefore allowing a higher efficiency when the photocathode is applied in solar to fuel devices. We have experimentally validated the photoelectrochemical performance of photocathodes consisting of nanoporous CuBO2 (CBO) on Fluorine‐doped Tin Oxide substrates, photosensitized with a light absorbing P1 dye. Femtosecond transient absorption and time‐resolved photoluminescence studies show that light‐induced hole injection occurs from the P1 dye into the CBO in a few ps, comparable to the time constant observed for NiO‐based photocathodes. Importantly, the CBO‐based photocathode shows significantly slower charge recombination than the NiO‐based analogue. These results illustrate the promise of CBO as a p‐type semiconductor in solar energy conversion devices.
DLX gene expression in the developing chick pharyngeal arches and relationship to endothelin signaling and avian jaw patterning
Authors: Sohail Afshan , Bendall Andrew J. 
Published: September 2023

AbstractBackgroundA hinged jaw that articulates with the skull base is a striking feature of the vertebrate head and has been greatly modified between, and within, vertebrate classes. Genes belonging to the DLX homeobox family are conserved mediators of local signaling pathways that distinguish the dorsal and ventral aspects of the first pharyngeal arch. Specifically, a subset of DLX genes are expressed in the cranial neural crest‐derived mandibular ectomesenchyme in response to ventral endothelin signaling, an important step that confers the first arch with maxillary and mandibular identities. Downstream targets of DLX genes then execute the morphogenetic processes that lead to functional jaws. Identifying lineage‐specific variations in DLX gene expression and the regulatory networks downstream of DLX action is necessary to understand how different kinds of jaws evolved.ResultsHere, we describe and compare the expression of all six DLX genes in the chick pharyngeal arches, focusing on the period of active patterning in the first arch. Disruption of endothelin signaling results in the down‐regulation of ventral‐specific DLX genes and confirms their functional role in avian jaw patterning.ConclusionsThis expression resource will be important for comparative embryology and for identifying synexpression groups of DLX‐regulated genes in the chick.
New species based on the biological species concept within the complex of Lariophagus distinguendus (Hymenoptera, Chalcidoidea, Pteromalidae), a parasitoid of household pests
Authors: Pollmann Marie , Kuhn Denise , König Christian , Homolka Irmela , Paschke Sina , Reinisch Ronja , Schmidt Anna , Schwabe Noa , Weber Justus , Gottlieb Yuval , Steidle Johannes Luitpold Maria 
Published: September 2023

AbstractThe pteromalid parasitoid Lariophagus distinguendus (Foerster) belongs to the Hymenoptera, a megadiverse insect order with high cryptic diversity. It attacks stored product pest beetles in human storage facilities. Recently, it has been shown to consist of two separate species. To further study its cryptic diversity, strains were collected to compare their relatedness using barcoding and nuclear genes. Nuclear genes identified two clusters which agree with the known two species, whereas the barcode fragment determined an additional third Clade. Total reproductive isolation (RI) according to the biological species concept (BSC) was investigated in crossing experiments within and between clusters using representative strains. Sexual isolation exists between all studied pairs, increasing from slight to strong with genetic distance. Postzygotic barriers mostly affected hybrid males, pointing to Haldane's rule. Hybrid females were only affected by unidirectional Spiroplasma‐induced cytoplasmic incompatibility and behavioural sterility, each in one specific strain combination. RI was virtually absent between strains separated by up to 2.8% COI difference, but strong or complete in three pairs from one Clade each, separated by at least 7.2%. Apparently, each of these clusters represents one separate species according to the BSC, highlighting cryptic diversity in direct vicinity to humans. In addition, these results challenge the recent ‘turbo‐taxonomy’ practice of using 2% COI differences to delimitate species, especially within parasitic Hymenoptera. The gradual increase in number and strength of reproductive barriers between strains with increasing genetic distance also sheds light on the emergence of barriers during the speciation process in L. distinguendus.
An optimized hybrid methodology for short‐term traffic forecasting in telecommunication networks
Authors: Alizadeh Mousa , Beheshti Mohammad T. H. , Ramezani Amin , Bolouki Sadegh 
Published: September 2023

AbstractWith the rapid development of telecommunication networks, the predictability of network traffic is of significant interest in network analysis and optimization, bandwidth allocation, and load balancing adjustment. Consequently, in recent years, significant research attention has been paid to forecasting telecommunication network traffic. Telecommunication traffic forecasting problems can be considered a time‐series problem, wherein periodic historical data is fed as the input to a model. Time‐series forecasting approaches are broadly categorized as statistical machine learning (ML) methods and their combinations. Statistical approaches forecast linear characteristics of time‐series data, unable to capture nonlinear and complex patterns. ML‐based approaches can model nonlinear characteristics of data. In recent years, hybrid approaches combining statistical and ML‐based approaches have been widely used to model linear and nonlinear data characteristics. However, the performance of these approaches highly depends on feature selection techniques and hyper‐parameter tuning of ML methods. A novel hybrid method is proposed for short‐term traffic forecasting based on feature selection and hyperparameter optimization to address this problem. It combines statistical and ML methods to model linear and nonlinear components of data. First, a novel feature selection technique, modified mutual information based on a linear combination of targets, is proposed to find the candidate input variables. Next, a combination of vector auto regressive moving average (VARMA), long short‐term memory (LSTM), and multilayer perceptron (MLP), called VARMA‐LSTM‐MLP forecaster, is suggested to forecast short‐term traffic. A hybrid metaheuristic algorithm, composed of firefly and BAT, is employed to find the optimal set of hyper‐parameter values. The proposed method is assessed by a real‐world dataset containing Tehran city's daily telecommunication data in IRAN. The evaluation results demonstrate that the proposed method outperforms the existing methods in terms of mean squared error and mean absolute error.
Preparation, characterization and properties of linalool and acyclic cucurbit[n]urils inclusion complexes
Authors: Zhou Chao , Zhang Shuqing , Li Yamin , Wang Lutao , Liu Qingmeng , Si Tian , Yang Bo 
Published: September 2023

AbstractLinalool is an important substance in the synthesis of fragrances owing to its unique aroma. However, the application of linalool is limited due to its low stability and rapid volatilization. Supramolecular inclusion technology plays an important role in improving the stability and volatility of fragrance and endows fragrance with excellent heat‐controlled release properties. In this study, inclusion complexes were prepared with two acyclic cucurbit[n]urils (M1 and M2) to improve the thermal stability of linalool, their binding behaviours were investigated by NMR, XRD, FT‐IR and Molecular Docking, confirming the formation of the host‐guest inclusion complexes. The stoichiometry of inclusion complexes was 1:1 by Job's plot and the binding constant was determined by fluorescence titration. The TG and heat‐controlled release experiment indicated that the inclusion complexes have higher thermal stability. This study provides a new idea and method for the development of linalool series spices.
Managing Expectations from Intensively Monitored Watershed Studies
Authors: Bisson Peter , Hillman Tracy , Beechie Tim , Pess George 
Published: September 2023

Abstract
Intensively monitored watershed (IMW) studies, the intent of which is to quantify habitat restoration effects on salmonid populations, have been underway in the Pacific Northwest, USA, for more than two decades. Lack of population-level response to habitat improvements by target species in some IMWs may be related to incomplete knowledge of factors regulating fish abundance, excessively prolonged restoration application periods, underappreciation of natural environmental and population variability, failure to carry out restoration at a sufficiently large scale within a watershed, lack of sufficient time to document a posttreatment response, or an actual failure of the restoration activities in those locations to achieve population recovery objectives. However, knowledge gained from IMWs has yielded important insight into (1) the long-term responses of salmon and steelhead Oncorhynchus mykiss to different types of restoration and (2) the importance of placing freshwater habitat improvements in the context of changes in anadromous salmonid survival and growth during other life history stages. Scientists, funding organizations, and policymakers should appreciate the potential value of IMWs as long-term barometers of the status of salmon populations and their habitats in watersheds where restoration activities are occurring. This requires a commitment to prolonged monitoring and an acknowledgment that environmental recovery after habitat restoration may take decades.
Synthesis of chromeno[4,3‐b]pyrrolidines from azomethine ylides and vinyl para‐quinone methides via (3 + 2) cycloaddition/oxa‐1,6‐addition
Authors: Wu Pei‐Shan , Liou Yan‐Cheng , Marri Gangababu , Chen Yi‐Ru , Wu Jia‐Rong , Lin Wenwei 
Published: September 2023

AbstractAn efficient synthesis of chromeno[4,3‐b]pyrrolidines has been successfully developed. This strategy involves a base‐catalyzed reaction of p‐VQMs and o‐hydroxy azomethine ylides via (3 + 2) cycloaddition/oxa‐1,6‐addition reaction sequence. The reaction condition is mild, and a series of chromeno[4,3‐b]pyrrolidines were obtained in 49‐95% yields with excellent diastereoselectivity (dr > 20:1).
Whole genome CRISPR screening strategy to identify genes contributing to SARS‐CoV‐2 spike and VSV‐G mediated entry
Authors: Hossain Md. Saddam , Kerkvliet Jason G. , Hoppe Adam D. 
Published: September 2023

AbstractUnderstanding the cellular host factors that promote and inhibit viral entry is important for identifying viral countermeasures. CRISPR whole‐genome screens can be used to rapidly discover host factors that contribute to or impair viral entry. However, when using live viruses and cellular lethality for selection, these screens can identify an overwhelming number of genes without specificity for the stage of the viral infection cycle. New screening methods are needed to identify host machinery contributing to specific steps of viral infection. Here, we developed a CRISPR whole‐genome screen and counter‐screen strategy based on a pseudoviral platform that allowed identification of genes specific to severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2) spike and vesicular stomatitis virus glycoprotein (VSV‐G) mediated entry. Screening of SARS‐CoV‐2 spike and VSV‐G on the same lentiviral pseudovirus allowed the identification of entry‐specific genes relative to genes associated with retro‐transcription, integration, and reporter expression from the lentiviral pseudovirus. Second, a Cre‐Gag fusion protein packaged into the pseudovirus was used to bypass retro‐transcription and integration by directly activating a floxed fluorescent protein reporter upon entry reduced the number of gene hits and increase specificity for viral entry. Our approach correctly identified SARS‐CoV‐2 and VSV‐G receptors ACE2 and low‐density lipoprotein receptors, respectively, and distinguished genes associated with retroviral reporter expression from envelope‐mediated entry. Moreover, the CRE‐Gag fusion/flox reporter increased the screen specificity for viral entry‐associated genes. Validation of a few hits demonstrates that this approach distinguishes envelope‐specific host factors from genes affecting reporter expression. Overall, this approach provides a new strategy for identifying host genes influencing viral entry without the confounding complexity of live‐viral screens which produce long gene lists associated with all aspects of viral pathogenesis and replication.
Role of human papillomavirus and associated viruses in bladder cancer: An updated review
Authors: Yao Xiangyang , Xu Zhenzhen , Duan Chen , Zhang Yangjun , Wu Xiaoliang , Wu Huahui , Liu Kai , Mao Xiongmin , Li Bo , Gao Yang , Xu Hua , Wang Xinghuan 
Published: September 2023

AbstractBladder cancer (BC) is a complex disease affecting the urinary system and is regulated by several carcinogenic factors. Viral infection is one such factor that has attracted extensive attention in BC. Human papillomavirus (HPV) is the most common sexually transmitted infection, and although multiple researchers have explored the role of HPV in BC, a consensus has not yet been reached. In addition, HPV‐associated viruses (e.g., human immunodeficiency virus, herpes simplex virus, BK virus, and JC virus) appear to be responsible for the occurrence and progression of BC. This study systematically reviews the relationship between HPV‐associated viruses and BC to elucidate the role of these viruses in the onset and progression of BC. In addition, the study aims to provide a greater insight into the biology of HPV‐associated viruses, and assess potential strategies for treating virus‐induced BC. The study additionally focuses on the rapid development of oncolytic viruses that provide a potentially novel option for the treatment of BC.
Risk factors associated with COVID‐19 pneumonia in Chinese patients with pre‐existing interstitial lung disease during the SARS‐CoV‐2 pandemic
Authors: Shao Chi , Shi Yujie , Chen Ruxuan , Liu Xiangning , Huang Hui , Zhao Yang , Xu Kai , Chen Keqi , Wang Mengzhao , Xu Zuojun 
Published: September 2023

AbstractIn China, the emergence of a nationally widespread epidemic infection of severe acute respiratory syndrome coronavirus 2 (SARS‐COV‐2) has appeared within a month since December 7, 2022. To evaluate the risk factors for suffering from coronavirus disease 2019 (COVID‐19) pneumonia due to infection with SARS‐CoV‐2 in different kinds of interstitial lung disease (ILD) patients with diverse immunizations, we conducted this retrospective study on 525 patients with ILDs who underwent regular follow‐up in our ILD clinic. Among them, 128 ILD patients (24.4%) suffered from COVID‐19 pneumonia after SARS‐CoV‐2 infection. Patients were older with a male predominance in the pneumonia group than in the nonpneumonia group (65.0 ± 10.0 years vs. 56.4 ± 11.7 years, p < 0.001, 55.5% vs. 39.5%, p = 0.002, respectively). Connective tissue disease‐associated ILD (CTD‐ILD) (25%), idiopathic pulmonary fibrosis (23.4%), and interstitial pneumonia with autoimmune features (21.1%) were the main pre‐existing ILDs in the pneumonia group. In Cox multivariable analysis, only male sex and corticosteroid use were risk factors for COVID‐19 pneumonia after infection. Two or three doses of vaccination were a protective factor for pre‐existing ILD patients suffering from COVID‐19 pneumonia. More than two doses of vaccination were strongly recommended for pre‐existing ILD patients, particularly for males who were administered corticosteroids.
A Mesostructure Multivariant‐Assembly Reinforced Ultratough Biomimicking Superglue
Authors: Xu Jin , Cong Qian , Zhao Tiancong 
Published: September 2023

AbstractThe imitation of mussels and oysters to create high‐performance adhesives is a cutting‐edge field. The introduction of inorganic fillers is shown to significantly alter the adhesive's properties, yet the potential of mesoporous materials as fillers in adhesives is overlooked. In this study, the first report on the utilization of mesoporous materials in a biomimetic adhesive system is presented. Incorporating mesoporous silica nanoparticles (MSN) profoundly enhances the adhesion of pyrogallol (PG)–polyethylene imine (PEI) adhesive. As the MSN concentration increases, the adhesion strength to glass substrates undergoes an impressive fivefold improvement, reaching an outstanding 2.5 mPa. The adhesive forms an exceptionally strong bond, to the extent that the glass substrate fractures before joint failure. The comprehensive tests involving various polyphenols, polymers, and fillers reveal an intriguing phenomenon—the molecular structure of polyphenols significantly influences adhesive strength. Steric hindrance emerges as a crucial factor, regulating the balance between π‐cation and charge interactions, which significantly impacts the multicomponent assembly of polyphenol‐PEI‐MSN and, consequently, adhesive strength. This groundbreaking research opens new avenues for the development of novel biomimetic materials.
Time‐efficient, high‐resolution3Twhole‐brain relaxometry using3D‐QALASwithwave‐CAIPIreadouts
Authors: Cho Jaejin , Gagoski Borjan , Kim Tae Hyung , Wang Fuyixue , Manhard Mary Kate , Dean Douglas , Kecskemeti Steven , Caprihan Arvind , Lo Wei‐Ching , Splitthoff Daniel Nico , Liu Wei , Polak Daniel , Cauley Stephen , Setsompop Kawin , Grant Patricia Ellen , Bilgic Berkin 
Published: September 2023

AbstractPurposeVolumetric, high‐resolution, quantitative mapping of brain‐tissue relaxation properties is hindered by long acquisition times and SNR challenges. This study combines time‐efficient wave–controlled aliasing in parallel imaging (wave‐CAIPI) readouts with the 3D quantification using an interleaved Look‐Locker acquisition sequence with a T2preparation pulse (3D‐QALAS), enabling full‐brain quantitative T1, T2, and proton density (PD) maps at 1.15‐mm3isotropic voxels in 3 min.MethodsWave‐CAIPI readouts were embedded in the standard 3D‐QALAS encoding scheme, enabling full‐brain quantitative parameter maps (T1, T2, and PD) at acceleration factors of R = 3 × 2 with minimum SNR loss due to g‐factor penalties. The quantitative parameter maps were estimated using a dictionary‐based mapping algorithm incorporating inversion efficiency and B1‐field inhomogeneity effects. The parameter maps using the accelerated protocol were quantitatively compared with those obtained from the conventional 3D‐QALAS sequence using GRAPPA acceleration of R = 2 in the ISMRM/NIST phantom, and in 10 healthy volunteers.ResultsWhen tested in both the ISMRM/NIST phantom and 10 healthy volunteers, the quantitative maps using the accelerated protocol showed excellent agreement against those obtained from conventional 3D‐QALAS at RGRAPPA = 2.ConclusionThree‐dimensional QALAS enhanced with wave‐CAIPI readouts enables time‐efficient, full‐brain quantitative T1, T2, and PD mapping at 1.15 mm3in 3 min at R = 3 × 2 acceleration. The quantitative maps obtained from the accelerated wave‐CAIPI 3D‐QALAS protocol showed very similar values to those from the standard 3D‐QALAS (R = 2) protocol, alluding to the robustness and reliability of the proposed method.
Relative quantification of progressive changes in healthy and dysferlin‐deficient mouse skeletal muscle proteomes
Authors: Golding Adriana E. , Li Wenping , Blank Paul S. , Cologna Stephanie M. , Zimmerberg Joshua 
Published: September 2023

AbstractIntroduction/AimsIndividuals with dysferlinopathies, a group of genetic muscle diseases, experience delay in the onset of muscle weakness. The cause of this delay and subsequent muscle wasting are unknown, and there are currently no clinical interventions to limit or prevent muscle weakness. To better understand molecular drivers of dysferlinopathies, age‐dependent changes in the proteomic profile of skeletal muscle (SM) in wild‐type (WT) and dysferlin‐deficient mice were identified.MethodsQuadriceps were isolated from 6‐, 18‐, 42‐, and 77‐wk‐old C57BL/6 (WT, Dysf+/+) and BLAJ (Dysf−/−) mice (n = 3, 2 male/1 female or 1 male/2 female, 24 total). Whole‐muscle proteomes were characterized using liquid chromatography‐mass spectrometry with relative quantification using TMT10plex isobaric labeling. Principle component analysis was utilized to detect age‐dependent proteomic differences over the lifespan of, and between, WT and dysferlin‐deficient SM. The biological relevance of proteins with significant variation was established using Ingenuity Pathway Analysis.ResultsOver 3200 proteins were identified between 6‐, 18‐, 42‐, and 77‐wk‐old mice. In total, 46 proteins varied in aging WT SM (p < .01), while 365 varied in dysferlin‐deficient SM. However, 569 proteins varied between aged‐matched WT and dysferlin‐deficient SM. Proteins with significant variation in expression across all comparisons followed distinct temporal trends.DiscussionProteins involved in sarcolemma repair and regeneration underwent significant changes in SM over the lifespan of WT mice, while those associated with immune infiltration and inflammation were overly represented over the lifespan of dysferlin‐deficient mice. The proteins identified herein are likely to contribute to our overall understanding of SM aging and dysferlinopathy disease progression.