bluuebunny/crossref_metadata_2025_split huggingface dataset:
Wdr5 is essential for fetal erythropoiesis and hematopoiesis
Authors: Liu Lulu , Fang Yanjia , Ding Xiaodan , Zhou Weihua , Terranova Remi , Zhang Yan , Wang He 
Published: April 2023

AbstractWDR5 is a highly conserved protein that performs multiple scaffolding functions in the context of chromatin. However, efforts to understand the function of WDR5 in normal tissues physiologically are quite limited so far. In our study, we explored the function of Wdr5 in erythropoiesis and hematopoiesis by using a hematopoietic-specific Wdr5 knockout mouse model. We found that loss of Wdr5 mediated by Vav-iCre leads to embryonic lethality with defective erythropoiesis. In addition, Wdr5-deficiency completely impairs the hematopoietic stem and progenitor cells function and might alter the immunophenotype of these stem cells and progenitors by decreasing c-Kit expression. Collectively, we identified the pivotal role of Wdr5 in fetal hematopoiesis and erythropoiesis as the de novo findings.
Characterisation of the koala (Phascolarctos cinereus) pouch microbiota in a captive population reveals a dysbiotic compositional profile associated with neonatal mortality
Authors: Maidment Toby I. , Bryan Emily R. , Pyne Michael , Barnes Michele , Eccleston Sarah , Cunningham Samantha , Whitlock Emma , Redman Kelsie , Nicolson Vere , Beagley Kenneth W. , Pelzer Elise 
Published: April 2023

Abstract

Background
Captive koala breeding programmes are essential for long-term species management. However, breeding efficacy is frequently impacted by high neonatal mortality rates in otherwise healthy females. Loss of pouch young typically occurs during early lactation without prior complications during parturition and is often attributed to bacterial infection. While these infections are thought to originate from the maternal pouch, little is known about the microbial composition of koala pouches. As such, we characterised the koala pouch microbiome across the reproductive cycle and identified bacteria associated with mortality in a cohort of 39 captive animals housed at two facilities.

Results
Using 16S rRNA gene amplicon sequencing, we observed significant changes in pouch bacterial composition and diversity between reproductive time points, with the lowest diversity observed following parturition (Shannon entropy — 2.46). Of the 39 koalas initially sampled, 17 were successfully bred, after which seven animals lost pouch young (overall mortality rate — 41.18%). Compared to successful breeder pouches, which were largely dominated by Muribaculaceae (phylum — Bacteroidetes), unsuccessful breeder pouches exhibited persistent Enterobacteriaceae (phylum — Proteobacteria) dominance from early lactation until mortality occurred. We identified two species, Pluralibacter gergoviae and Klebsiella pneumoniae, which were associated with poor reproductive outcomes. In vitro antibiotic susceptibility testing identified resistance in both isolates to several antibiotics commonly used in koalas, with the former being multidrug resistant.

Conclusions
This study represents the first cultivation-independent characterisation of the koala pouch microbiota, and the first such investigation in marsupials associated with reproductive outcomes. Overall, our findings provide evidence that overgrowth of pathogenic organisms in the pouch during early development is associated with neonatal mortality in captive koalas. Our identification of previously unreported, multidrug resistant P. gergoviae strains linked to mortality also underscores the need for improved screening and monitoring procedures aimed at minimising neonatal mortality in future.
New insights into the immune functions of podocytes: the role of complement
Authors: Bruno Valentina , Mühlig Anne Katrin , Oh Jun , Licht Christoph 
Published: April 2023

AbstractPodocytes are differentiated epithelial cells which play an essential role to ensure a normal function of the glomerular filtration barrier (GFB). In addition to their adhesive properties in maintaining the integrity of the filtration barrier, they have other functions, such as synthesis of components of the glomerular basement membrane (GBM), production of vascular endothelial growth factor (VEGF), release of inflammatory proteins, and expression of complement components. They also participate in the glomerular crosstalk through multiple signalling pathways, including endothelin-1, VEGF, transforming growth factor β (TGFβ), bone morphogenetic protein 7 (BMP-7), latent transforming growth factor β-binding protein 1 (LTBP1), and extracellular vesicles.Growing literature suggests that podocytes share many properties of innate and adaptive immunity, supporting a multifunctional role ensuring a healthy glomerulus. As consequence, the “immune podocyte” dysfunction is thought to be involved in the pathogenesis of several glomerular diseases, referred to as “podocytopathies.” Multiple factors like mechanical, oxidative, and/or immunologic stressors can induce cell injury. The complement system, as part of both innate and adaptive immunity, can also define podocyte damage by several mechanisms, such as reactive oxygen species (ROS) generation, cytokine production, and endoplasmic reticulum stress, ultimately affecting the integrity of the cytoskeleton, with subsequent podocyte detachment from the GBM and onset of proteinuria.Interestingly, podocytes are found to be both source and target of complement-mediated injury. Podocytes express complement proteins which contribute to local complement activation. At the same time, they rely on several protective mechanisms to escape this damage. Podocytes express complement factor H (CFH), one of the main regulators of the complement cascade, as well as membrane-bound complement regulators like CD46 or membrane cofactor protein (MCP), CD55 or decay-accelerating factor (DAF), and CD59 or defensin. Further mechanisms, like autophagy or actin-based endocytosis, are also involved to ensure podocyte homeostasis and protection against injury.This review will provide an overview of the immune functions of podocytes and their response to immune-mediated injury, focusing on the pathogenic link between complement and podocyte damage.
Tumor lineage-specific immune response in brain metastatic disease: opportunities for targeted immunotherapy regimen?
Authors: Najjary Shiva , Kros Johan M. , de Koning Willem , Vadgama Disha , Lila Karishma , Wolf Janina , Mustafa Dana A. M. 
Published: April 2023

AbstractMetastases in the brain are the most severe and devastating complication of cancer. The incidence of brain metastasis is increasing. Therefore, the need of finding specific druggable targets for brain metastasis is demanding. The aim of this study was to compare the brain (immune) response to brain metastases of the most common tumor lineages, viz., lung adenocarcinoma and breast cancer. Targeted gene expression profiles of 11 brain metastasis of lung adenocarcinoma (BM-LUAD) were compared to 11 brain metastasis of breast cancer (BCBM) using NanoString nCounter PanCancer IO 360™ Panel. The most promising results were validated spatially using the novel GeoMx™ Digital Spatial Profiler (DSP) Technology. Additionally, Immune cell profiles and expression of drug targets were validated by multiplex immunohistochemistry. We found a more active immune response in BM-LUAD as compared to BCBM. In the BM-LUAD, 138 genes were upregulated as compared to BCBM (adj. p ≤ 0.05). Conversely, in BCBM 28 genes were upregulated (adj. p ≤ 0.05). Additionally, genes related to CD45 + cells, T cells, and cytotoxic T cells showed to be expressed higher in BM-LUAD compared to BCBM (adj. p = 0.01, adj. p = 0.023, adj. p = 0.023, respectively). The spatial quantification of the immune cells using the GeoMx DSP technique revealed the significantly higher quantification of CD14 and CD163 in tumor regions of BM-LUAD as compared to BCBM. Importantly, the immune checkpoint VISTA and IDO1 were identified as highly expressed in the BM-LUAD. Multiplex immunohistochemistry confirmed the finding and showed that VISTA is expressed mainly in BM-LUAD tumor cells, CD3 + cells, and to fewer levels in some microglial cells in BM-LUAD. This is the first report on differences in the brain immune response between metastatic tumors of different lineages. We found a far more extensive infiltration of immune cells in BM-LUAD as compared to BCBM. In addition, we found higher expression of VISTA and IDO1 in BM-LUAD. Taken together, targeted immune therapy should be considered to treat patients with BM-LUAD.
Characterization of landslides: a vertical electrical sounding approach
Authors: Christopher W. A. P. P. , De Silva Nalin , Attanayake A. M. A. N. B. , Jayasingha Pathmakumara 
Published: April 2023

AbstractThe high groundwater activity typically found in landslide bodies favors electrical resistivity methods to characterize the subsurface of landslides. The more water present, the more the current injection into the ground and thus greater the depth of penetration. Furthermore, this generates a higher contrast between the stable and the unstable mass of the landslide. Being a cost-effective and a flexible approach to characterize landslides, 1-D electrical resistivity surveys have the ability to acquire point data similar to borehole logs, thus allows to detect and highlight anomalies of groundwater flow and groundwater build up. These parameters could change from point to point due to the inherit disturbed nature of landslides. A preliminary vertical electrical sounding survey using Schlumberger electrode configuration was carried out along the axis of a creep-type landslide in Badulusirigama, Badulla, Sri Lanka. Four survey points were established for the preliminary VES survey. Each survey line spanned 140 m in length. The data revealed the presence of two types of layers with high (200–370 Ω m) and low (20–60 Ω m) apparent resistivities. Another resistive layer with an apparent resistivity around 100–140 Ω m was found to be sandwiched in the clay layer which was significant to the landslide body and interestingly was not observed in the borehole logs. Furthermore, the southwest inclination of this sandwiched layer explained the southwest region of the landslide being highly active in terms of groundwater discharge through horizontal drains. The bedrock was not detected at three of the survey points highlighting the thick clay layer present in the landslide body. This preliminary survey revealed the general anatomy of the landslide along its axis in terms of the number of layers, type of layers, their thicknesses, the presence of water, and their resistivities.
Isokinetic knee muscle strength comparison after enhanced recovery after surgery (ERAS) versus conventional setup in total knee arthroplasty (TKA): a single blinded prospective randomized study
Authors: Goetz Julia , Maderbacher Günther , Gerg Anna , Leiss Franziska , Dullien Silvia , Zeman Florian , Meyer Matthias , Reinhard Jan , Grifka Joachim , Greimel Felix 
Published: April 2023

AbstractPurposeTotal knee arthroplasty (TKA) combined with the concept of enhanced recovery is of continued worldwide interest, as it is reported to improve early functional outcome and treatment quality without increasing complications. The aim of the study was to investigate isokinetic knee muscle strength after cemented TKA in combination with an enhanced recovery after surgery (ERAS) compared to a conventional setup.MethodsIn the single blinded prospective randomized study, 52 patients underwent navigated primary cemented TKA within an ERAS (n = 30) or a conventional setup (n = 22). Preoperatively, five days and four weeks after surgery isokinetic knee muscle strength with BIODEX‐type measuring device (peak torque in Nm, work in Joules and power in Watt) and subjective patient‐related outcome measures (PROMs) were investigated.ResultsThe ERAS group showed significantly better outcomes in knee flexion at 180°/s (peak torque (Nm) p = 0.047, work (J) p = 0.040 and power (W) p = 0.016) 5 days postoperatively. The isokinetic measuring at knee extension 60°/s and 180°/s demonstrated no significant difference. The PROMs showed that patients were satisfied with the postoperative results in both groups. After 4 weeks, there was no longer a significant difference in isokinetic measuring at knee extension and flexion between the ERAS and conventional group.ConclusionsTKA with the concept of ERAS improves excellent isokinetic outcome and patient satisfaction. The isokinetic muscle strength measurement can help patients and surgeons to modify expectations and improve patient satisfaction.
Optimizing internal biosecurity on pig farms by assessing movements of farm staff
Authors: Bernaerdt Elise , Díaz Inmaculada , Piñeiro Carlos , Collell Miquel , Dewulf Jeroen , Maes Dominiek 
Published: April 2023

AbstractFor internal biosecurity, it is important to separate different age groups in a pig farm and to stick to specific working lines when visiting the barns. Currently, there is no research on the movements of farm staff on pig farms. The objectives of this observational study were to assess movements of farm staff on pig farms, to assess risky movements and to investigate whether movements differ according to time (week of the batch farrowing system (BFS) and weekday vs. weekend) and unit (farrowing, gestation/insemination, nursery, and fattening unit). Five commercial sow farms participated and on each farm, an internal movement monitoring system was installed. Detection points were installed throughout the farm and workers had to wear a personal beacon. Movement data were collected from 1 December 2019 until 30 November 2020. The following sequence of movements was considered as safe: (1) dressing room, (2) farrowing, (3) gestation/insemination, (4) nursery, (5) fattening, (6) quarantine, and (7) cadaver storage. Movements in the opposite direction were considered as risk, unless a dressing room was visited in between. The total number of movements differed according to week of the BFS, and was highest in insemination and farrowing week. The percentage of risky movements was influenced by week of the BFS for two farms, and was highest around weaning. The percentage of risky movements varied between farms and ranged from 9 to 38%. There were more movements on a weekday compared to a weekend day. There were more movements towards the farrowing and gestation/insemination unit in insemination and farrowing week compared to other weeks of the BFS, but week of the BFS had no impact on movements towards nursery and fattening unit. This study showed that there were a lot of (risky) movements on pig farms and that these movements varied according to week of the BFS, day of the week, and unit. This study creates awareness, which could be a first step in optimizing working lines. Future research should focus on why certain risky movements occur and how these can be avoided to achieve better biosecurity and higher health status on farms.
Responsive manganese-based nanoplatform amplifying cGAS-STING activation for immunotherapy
Authors: He Qingbin , Zheng Runxiao , Ma Junchi , Zhao Luyang , Shi Yafang , Qiu Jianfeng 
Published: April 2023

Abstract

Background
The activation of the cyclic guanosine monophosphate-adenosine monophosphate synthase-stimulator of interferon genes (cGAS-STING) signaling pathway has attracted great attention for its ability to up-regulate innate immune response and thus enhance cancer immunotherapy. However, many STING agonists limit the further advancement of immunotherapy due to weak tumor responsiveness or low activation efficiency. The responsive and effective activation of cGAS-STING signaling in tumors is a highly challenging process.

Methods

In this study, a manganese-based nanoplatform (MPCZ NPs) was constructed that could responsively and efficiently generate more manganese ions (Mn
2+
) and reactive oxygen species (ROS) to activate cGAS-STING signaling pathway. Briefly, manganese dioxide (MnO
2
) was loaded with zinc protoporphyrin IX (ZPP) molecule and coated by polydopamine (PDA) embedded with NH
4
HCO
3
to obtain MPCZ NPs. Additionally, MPCZ NPs were evaluated in vitro and in vivo for their antitumor effects by methyl thiazolyl tetrazolium (MTT) assay and TUNEL assays, respectively.

Results

In this system, tumor responsiveness was achieved by exogenous (laser irradiation) and endogenous (high levels GSH) stimulation, which triggered the collapse or degradation of PDA and MnO
2
. Moreover, the release of Mn
2+
augmented the cGAS-STING signaling pathway and enhanced the conversion of hydrogen peroxide (H
2
O
2
) to hydroxyl radical (·OH) under NIR laser irradiation. Furthermore, the release of ZPP and the elimination of GSH by MPCZ NPs inhibited HO-1 activity and prevented ROS consumption, respectively.

Conclusions

This adopted open source and reduce expenditure strategy to effectively generate more ROS and Mn
2+
to responsively activate cGAS-STING signaling pathway, providing a new strategy for improving immunotherapy.
Salty taste test for a low-salt diet to control blood pressure
Authors: Yang Seon-Hee , Ha Jea-Chul , Kim Min-Ji 
Published: April 2023

Abstract
Background
Reducing salt intake helps prevent complications of cerebrovascular disease. To help patients accept a low-sodium diet, the salty taste test is used to evaluate how much salt an individual actually consumes. The aim of this study was to help patients with hypertension reduce their salt intake by helping them recognize the difference between their subjective perception of saltiness and the objective test results.

Methods
We enrolled workers who visited a local occupational health institution in the period from April to August 2019. Demographic and physical characteristics were recorded. Blood pressure measurement and use of medication were also recorded. A questionnaire was used to investigate whether people liked or disliked salty food, i.e., preference for saltiness, and whether they usually ate salty, normal, or fresh food, i.e., the subjective perception of saltiness. Subsequently, the taste determination kit provided by the Ministry of Food and Drug Safety was used to objectively test saltiness at various salty taste concentrations. The Ministry of Food and Drug Safety program (No. 10-093760) was used as the salty taste judgment tool.

Results
A total of 86 workers were surveyed. Eleven of 18 workers (61.1%) who reported usually eating fresh food actually ate normal or salty food. Thirteen of 37 workers (35.1%) who reported eating normal food actually ate salty food. Thirteen of 31 workers (41.9%) who reported eating salty food actually ate fresh or normal food. Of 46 workers who reported disliking salty food, 14 (30.4%) actually ate salty food, while 20 (43.5%) ate normal food. The subjective perception and preference for saltiness were not significantly correlated with the objective test results (P = ​​0.085 and P = 0.110, respectively). As for the subjective perception and preference for saltiness, Cohen’s weighted kappa for the taste judgment result were 0.23 and 0.22, respectively, indicating a low degree of agreement.

Conclusions
In dietary counseling to prevent cerebrovascular and cardiovascular diseases, rather than relying on the subjective perception of saltiness, a salty taste test should be performed such that people can recognize their salty food eating habits through objective evaluation.
Bibliometric mapping of the landscape and structure of nutrition and depression research: visualization analysis
Authors: Zyoud Sa’ed H. , Shakhshir Muna , Abushanab Amani S. , Koni Amer , Shahwan Moyad , Jairoun Ammar A. , Al-Jabi Samah W. 
Published: April 2023

Abstract
Background
Numerous epidemiological studies have examined the relationship between dietary intake of specific foods or nutrients and the incidence of depression and have noted that nutrition has a significant impact on mental health. Therefore, the purpose of this study is to assess the state of research, the frontiers of research, and development trends in the field of nutrition and depression using bibliometric and visual analysis.

Methods
We collected publications on the topic of nutrition and depression from Scopus between 2002 and 2021. Subsequently, we utilized VOSviewer 1.6.18 and Microsoft Excel 2013 to perform bibliometric analysis and visualization. Bibliometric analysis involves retrieving documents from a singular database, such as SciVerse Scopus or Web of Knowledge, and subjecting them to quantitative and qualitative analysis. Notably, gray literature is not considered in bibliometric analysis.

Results
A total of 2171 publications on nutrition and depression were found between 2002 and 2021, namely 1855 (85.44%) original articles, 190 (8.75%) reviews, 38 (1.75%) letters, and 88 (4.05%) other types of publications. The most productive country was found to be the USA (n = 726; 33.44%), followed by Australia (n = 172; 7.92%), the United Kingdom (n = 158; 7.28%), China (n = 132; 6.08%), and Canada (n = 131; 6.03%). The remaining publications were from other countries (n = 852; 39.25%). According to the citation analysis, the retrieved papers were cited on an average of 26.6 times and had an h-index of 105 with 57,781 citations. The most frequent terms on the map include those related to (a) fatty acid links to depression and brain inflammation, (b) depression and eating disorders, and finally, (c) adherence to the Mediterranean diet and risk of depression.

Conclusions
The current study was the first novel bibliometric analysis of nutrition and depression research that used data extracted from Scopus for visualization network mapping. In recent years, the theme "Mediterranean diet adherence and risk of depression" has been identified more frequently, indicating that studies in this field have garnered considerable attention and reflect the most recent scientific advances. Researchers should continue to investigate nutrition and depression, and we believe this study provides significant information for researchers, nutritionists, and clinicians.
When less is not more: the effect of transparent masks on facial attractiveness judgment
Authors: Lee Yongseong , Jeong Su Keun 
Published: April 2023

AbstractDuring the COVID-19 pandemic, face masks have been widely used in daily life. Previous studies have suggested that faces wearing typical masks that occlude the lower half of the face are perceived as more attractive than face without masks. However, relatively little work has been done on how transparent masks that reveal the lower half of the face affect the judgment of facial attractiveness. To investigate the effect of transparent masks on the perceived attractiveness, in the current study, we asked participants to rate the attractiveness of faces without masks and with a typical opaque mask and a transparent mask. The results showed that faces wearing opaque masks were evaluated as more attractive than those wearing transparent masks or no masks. The benefit of opaque masks was more pronounced in faces that were initially evaluated as unattractive. Interestingly, wearing transparent masks decreased the perceived attractiveness of faces but only for the faces initially rated as attractive, possibly because of the visual distortion of the lower half of the face by transparent masks. In summary, we found that opaque and transparent masks have different effects on perceived attractiveness, depending on the attractiveness of faces. Given benefits of transparent masks in socio-emotional and cognitive processing, it would be important to further understand the effect of transparent masks on face information processing.