bluuebunny/crossref_metadata_2025_split huggingface dataset:
Primary and specialized metabolisms of Triticum aestivum L. affected by Solieria chordalis
Authors: Ducatti Rafael , Filho João Américo Wordell , Tironi Siumar Pedro , Mazaro Sergio Miguel 
Published: April 2023

Abstract

Plant elicitation provides multi-benefits to plant development and defense, besides helping to make agriculture more sustainable. However, the use of wrong experimental designs and technics directly affects the results obtained. This favors the generation of biased and unreliable data. Therefore, an investigation was conducted to assess the eliciting potential of a red seaweed biostimulant (RSB) alone or in combination with fungicide in total controlled conditions on wheat (Triticum aestivum L.) plants. Catalase, superoxide dismutase, ascorbate peroxidase, phenylalanine ammonia-lyase (PAL), salicylic acid (SA) content, powdery mildew incidence, photosynthetic rates, and the water use efficiency were assessed in different periods after the application of the elicitor. The use of the RSB prevented the incidence of powdery mildew in wheat plants. The fungicide alone or in combination with the RSB resulted in negative photosynthetic rates and downregulated the activity of some enzymes. A greater PAL activity did not warrant a greater accumulation of SA in plants. Overall, the use of fungicide negatively affected wheat’s primary and specialized metabolisms when compared to the application of the RSB alone.
Quantum speed limit for complex dynamics
Authors: Zhang Mao , Yu Huai-Ming , Liu Jing 
Published: April 2023

Abstract
Quantum speed limit focuses on the minimum time scale for a fixed mission and hence is important in quantum information where fast dynamics is usually beneficial. Most existing tools for the depiction of quantum speed limit are the lower-bound-type tools, which are in fact difficult to reveal the true minimum time, especially for many-body systems or complex dynamics. Therefore, the evaluation of this true minimum time in these scenarios is still an unsolved problem. Hereby we propose a three-step (classification-regression-calibration) methodology based on machine learning to evaluate the true minimum time in complex dynamics. Moreover, the analytical expression of the true minimum time is also provided for the time-dependent Hamiltonians with time-independent eigenstates.
Incentive or constraint? Comprehensive impacts of green credit policy on the industrial enterprises' energy intensity
Authors: Li Jinkai , Zhang Can , ZHANG JIN , Mi Zhifu , Liu Zhuang , Gong Liutang , Lu Gang 
Published: April 2023

Abstract
Discussing the function of green credit policy (GCP), which has dual attributes of being both a “environmental regulation” and a “financial instrument”; is crucial for the process of industrial green transformation. However, there is limited theoretical and empirical evidence on the impact of GCP on green transformation of industry. This research fills this gap by exploring the comprehensive impacts and mechanisms of GCP on the energy intensity (EI) of industrial enterprises in China. Theoretically, the environmental and financial impacts of GCP are introduced into a unified analytical framework on the basis of the heterogeneous enterprise model. Empirically, diverse empirical methods, such as difference-in-differences (DID), difference-in-differences-in-differences (DDD), and mediating effect models, are adopted to examine whether GCP can promote green innovation or accelerate financial constraint. The overall result shows that: (1) GCP has significantly decreased the EI of industrial enterprises. The impact of incentives was far greater than that of constraints. (2) In the incentive effect, energy substitution and innovation offsets exerted a primary influence on reducing the EI of industrial enterprises. (3) The constraint effect was primarily caused by rising financing and pollution abatement costs. This study offers a playbook for policymakers in China and other emerging economies to design effective policies for promoting industrial green transformation and sustainable production.
The effect of nurturing care interventions on early child development and care: findings from a quasi-experimental study in a humanitarian setting
Authors: Sargsyan Viktoria , Tenorio Ana , Uwera Mediatrice , Gasirikare Andre , Habyarimana Jean Aime , Salcido Jennifer S , Felner Christy , Rasheed Muneera A 
Published: April 2023

Abstract

Background
The objective of the study to determine if a nurturing care parenting programme delivered in a humanitarian setting in Rwanda to determine would benefit early development, learning and care outcomes for young children under 5 years and their caregivers compared to standard care.
Methodology
The parenting programme was implemented in Rwanda’s Mugombwa, Kansi and Kigeme refugee camp and host communities. Via a quasi-experimental research design, the study assessed the effects of intervention delivered as high dose (HD: 12 group sessions and 4 home visits) or low dose (LD: 6 group sessions and 2 home visits) on child and caregiver outcomes compared to the control group from similar settings receiving standard care. Child development outcomes were assessed using the Ages and Stages Questionnaires (ASQ). Parenting practices with respect to early learning and stimulation were assessed using standard questionnaires.
Findings
A total of 733 children and families were assessed: HD = 314, LD = 240, control = 179. No significant difference on child development scores between intervention and control groups was found. Caregivers exposed to HD and LD packages had significantly higher scores on early learning and stimulation practices compared to the control group respectively with 211(67.2%), 148 (61.7%) vs. 66 (36.9%), p < .001 caregivers engaged in 4 or more activities in the past 3 days. Similarly on responsive feeding items, a higher percentage of HD and LD group caregivers were engaged in positive behaviours compared to control group: 164 (52.2%), 108 (45%) vs 62 (34.6%), p = 0.001. No differences were found in the study arms in regard to caregiver mental health.
Conclusion
Parenting programmes in humanitarian settings can positively impact on nurturing care practices, even with a low dose, which are essential to strengthening children’s resilience living in at-risk conditions such as refugee settings. Further studies in such context are essential to strengthen the proof of concept from the study findings.
Predictive Value of Preoperative Computed Tomography for Lateral Pelvic Lymph Node Metastasis in Rectal Cancer
Authors: Emoto Shin , Homma Shigenori , Yoshida Tadashi , Ichikawa Nobuki , Matsui Hiroki , Tani Michio , Fujima Noriyuki , Taketomi Akinobu 
Published: April 2023

Abstract
Background: Pretreatment diagnosis of lateral pelvic lymph node (LPLN) metastasis is critical in deciding the therapeutic strategy for locally advanced rectal cancer. We aimed to investigate computed tomography (CT) cut-off values and diagnostic and prognostic implications of lateral pelvic lymph node metastasis (LPLNM) in rectal cancer.
Methods: Patients with rectal cancer (n=126) who underwent primary tumor resection between April 2010 and July 2020 were included. CT findings and diagnostic accuracies were analyzed.
Results: In this study, the cut-off values of 7 mm for long-axis diameter and 5 mm for short-axis diameter were selected. At cut-offs of 7 mm for long-axis diameter the values of sensitivity, specificity, positive predictive value, negative predictive value, and accuracy were 88.9%, 93.8%, 34.8%, 99.6%, and 93.7%, respectively. At cut-offs of 5 mm for short-axis diameter, the values of sensitivity, specificity, positive predictive value, negative predictive value, and accuracy were 88.9%, 90.9%, 26.7%, 99.5%, and 90.9%, respectively. CT findings of ≥7 mm for long-axis diameter (odds ratio, 68.0) and ≥5 mm for short-axis diameter (odds ratio, 38.4) were strong predictors of LPLNM. In subgroup analysis of patients with enlarged LPLN (≥7 mm), higher recurrence-free survival and lower local recurrence rates were found in patients who had LPLN dissection (5-year recurrence-free survival: 100% vs. 58.3%, p=0.17 with LPLN dissection; and 5-year local recurrence: 0% vs. 31.4%, p=0.27, without LPLN dissection).
Conclusion: Pretreatment CT at cut-off values of 7 mm for long-axis diameter and 5 mm for short-axis diameter of LPLN provides sufficient diagnostic accuracy for LPLNM.
Overcoming multidrug resistance by reversan and exterminating glioblastoma and glioblastoma stem cells by delivering drug loaded nanostructure hybrid lipid capsules (nHLCs)
Authors: Hasan Uzma , Chauhan Meenakshi , Basu Suparna Mercy , R Jayakumar , Giri Jyotsnendu 
Published: April 2023

AbstractGlioblastoma multiforme (GBM) is regarded as highly aggressive brain cancer with poor prognosis. There is increase in expression of P-glycoprotein (P-gp), responsible for multidrug resistance (MDR), making it a potential target for improving drug responses. Additionally, presence of glioblastoma stem cells (GSCs) increases resistance to chemo- and radiotherapy and play a major role in cancer relapse. In this study, we targeted P-gp using a small molecule inhibitor, reversan (RV), to inhibit MDR that prolonged the retention of drugs in the cytosolic milieu. For elimination of GBM and GSCs, we have used two well established anti-cancer drugs, regorafenib (RF) and curcumin (CMN). To improve the pharmacokinetics and decrease systemic delivery of drugs, we developed nanostructure hybrid lipid capsules (nHLCs), where hydrophobic drugs can be loaded in the core and theie physicochemical properties were determined by dynamic light scattering (DLS) and cryo-scanning electron microscopy (SEM). Inhibition of MDR by RV has also shown enhanced retention of nHLC in GBM cells. Co-delivery of drug loaded nHLCs, pre-treated with RV, exhibited superior cytotoxicity in both GBM and GSCs than their individual doses and effectively reduced size and stemness of tumor spheres and accelerated the rate of apoptosis suggesting a promising treatment for glioblastoma.
Nascent Chains Derived from a Foldable Protein Sequence Interact with Specific Ribosomal Surface Sites near the Exit Tunnel
Authors: Masse Meranda M. , Guzman-Luna Valeria , Varela Angela E. , Hutchinson Rachel B. , Srivast Aniruddha , Wei Wanting , Fuchs Andrew M. , Cavagnero Silvia 
Published: April 2023

Abstract

In order to become bioactive, proteins must be translated and protected from aggregation during biosynthesis. The ribosome and molecular chaperones play a key role in this process. Ribosome-bound nascent chains (RNCs) of intrinsically disordered proteins and RNCs bearing a signal/arrest sequence are known to interact with ribosomal proteins. However, in the case of RNCs bearing foldable protein sequences, no direct information is available on these interactions. Here, via a combination of chemical crosslinking and time-resolved fluorescence-anisotropy, we find that nascent chains of the foldable globin apoHmp1 − 140 interact with ribosomal protein L23 and have a freely-tumbling non-interacting N-terminal compact region comprising 63–94 residues. Longer RNCs (apoHmp1 − 189) also interact with an additional yet unidentified ribosomal protein, as well as with chaperones. Surprisingly, the apparent strength of RNC/r-protein interactions does not depend on nascent-chain sequence. Overall, foldable nascent chains establish and expand interactions with selected ribosomal proteins and chaperones, as they get longer. These data are significant because they reveal the interplay between independent conformational sampling and nascent-protein interactions with the ribosomal surface.
Effectiveness of biological nurturing on maternal breastfeeding problems: a systematic review and meta-analysis.
Authors: hu longyi , zhou leshan , zhou ziyun 
Published: April 2023

Abstract
Background Breastfeeding is important for maternal and child health, yet the rate of exclusive breastfeeding for the first 6 months after birth is low worldwide. Feeding posture is one of the factors that affect breastfeeding. Several articles on biological nurturing have shown that It can improve breastfeeding, but the evaluation metrics of these articles vary and no uniform standards.Research Aims To investigate the influence of biological nurturing on breastfeeding.Method Cochrane library, Embase, PubMed, web of science, Medline, EBSCO, CBM, CNKI, VIP, WANFANG were systematically searched for studies that met criteria. Two investigators independently assessed the quality of the selected literature and screened data; 14 studies involving 2547 participants were included and meta-analysis was performed using revman 5.3 and state15.1.Results The results showed that the observation group compared to the control group in terms were statistically different, the incidence of exclusive breastfeeding [RR = 1.16,95%CI=[1.10,1.23],P < 0.001], the incidence of breastfeeding success [RR = 1.23,95%CI=[1.13,1.33],P < 0.001], maternal satisfaction [effect size = 1.22,95%CI=[1.16, 1.28],P < 0.001], mental status scale in non-psychiatric settings (MSSNS) score [SMD=-3.05,95%CI=[-3.44,-2.65],P < 0.001], breast pain condition [RR = 0.72.95%CI=[0.61, 0.85],P < 0.001], breastfeeding self-efficacy [SMD = 0.69,95%CI [0.09,1.29]=,P = .02],lactation initiation time[SMD=-1.50, 95%CI=[-2.65, -0.36],P = .01], and postural comfort [SMD = 0.85, 95%CI=[0.68, 1.02],P < 0.001], indicating that the biological nurturing method had a positive effect.Conclusions: Biological nurturing has been shown to better facilitate postpartum women in increasing exclusive breastfeeding incidence, the chance of successful breastfeeding, improving maternal psychological state, maternal satisfaction, maternal breastfeeding self-efficacy and reducing breast pain and breast injury, compared to traditional breastfeeding positions. There is little research on the effects of biologic nurturing on infants, which could be further explored.
Extensive freshened groundwater resources emplaced during the Messinian sea-level drawdown: Insights from southern Sicily (Italy)
Authors: Lipparini Lorenzo , Chiacchieri Damiano , Bencini Roberto , Micallef Aaron 
Published: April 2023

Abstract
Deep groundwater resources around the world represent an important potential unconventional source of water. Here we document an extensive (17.3 km3) fresh/brackish groundwater body preserved in a deep (between 800 and 2100 m) carbonate platform aquifer in southern Sicily (Italy), by using deep well data and a 3D hydrogeological modelling. We attribute the distribution of this fossil groundwater to topographically-driven meteoric recharge driven by the Messinian sea-level drawdown, which we estimate to have reached 2400 m below present sea level in the eastern Mediterranean Basin. The discovery of such an extensive and deep freshened groundwater has significant implications in terms of resource potential for southern Sicily and other Mediterranean coastal regions, that share similar geological setting and water scarcity issues.
Capicua (CIC) mutations in gliomas in association with MAPK activation for exposing a potential therapeutic target
Authors: Darabi Sourat , Xiu Joanne , Samec Timothy , Kesari Santosh , Carrillo Jose , Aulakh Sonikpreet , Walsh Kyle M. , Sengupta Soma , Sumrall Ashley , Spetzler David , Glantz Michael , Demeure Michael J 
Published: April 2023

Abstract
Gliomas are the most prevalent neurological cancer in the United States and care modalities are not able to effectively combat these aggressive malignancies. Identifying new, more effective treatments require a deep understanding of the complex genetic variations and relevant pathway associations behind these cancers. Drawing connections between gene mutations with a responsive genetic target can help drive therapy selections to enhance patient survival. We have performed extensive molecular profiling of the Capicua gene (CIC), a tumor and transcriptional suppressor gene, and its mutation prevalence in reference to MAPK activation within clinical glioma tissue. CIC mutations occur far more frequently in oligodendroglioma (52.1%) than in low-grade astrocytoma or glioblastoma. CIC-associated mutations were observed across all glioma subtypes, and MAPK-associated mutations were most prevalent in CIC wild-type tissue regardless of the glioma subtype. MAPK activation, however, was enhanced in CIC-mutated oligodendroglioma. The totality of our observations reported supports the use of CIC as a relevant genetic marker for MAPK activation. Identification of CIC mutations, or lack thereof, can assist in selecting, implementing, and developing MEK/MAPK-inhibitory trials to improve patient outcomes potentially.
Revision Anterior Cruciate Ligament Reconstruction with Over-the-top Augmentation Technique Using an Achilles Tendon Allograft for Enhanced Rotational Stability
Authors: Lim Sumin , Park Ki-Hoon , Park Do Young , Kim Tae Hun , Koh Jeong-Hyun , Chung Jun Young 
Published: April 2023

Abstract
Purpose
Revision anterior cruciate ligament (ACL) reconstruction is technically challenging due to mispositioned tunnels, bone loss, and tunnel expansion, which may compromise graft fixation or strength and result in failure. To obtain firm graft fixation and strength in one stage, we developed a new over-the-top augmentation technique using an Achilles tendon allograft in revision ACL reconstruction (OA-ACLR). This study compared OA-ACLR with single-bundle ACL reconstruction (SB-ACLR). We hypothesized that OA-ACLR would enhance the postoperative knee joint rotational stability.
Methods
We retrospectively analyzed 47 patients who underwent revisional OA-ACLR and 48 who underwent primary SB-ACLR. Knee instability was evaluated with the anterior drawer, Lachman, and pivot shift tests preoperatively and at the final follow-up. Side-to-side differences were compared with the non-affected side at the final follow-up. Function was evaluated using the IKDC subjective and Lysholm knee scores preoperatively and at the final follow-up.
Results
The groups did not differ in terms of sex, age, and BMI. Both groups significantly improved in the anterior drawer, Lachman, pivot shift tests, and IKDC and Lysholm knee scores after surgery (all p < 0.001). The OA-ACLR group showed significantly higher rotational stability in the pivot shift test than the SB-ACLR group (p = 0.017). The postoperative side-to-side difference, the IKDC and Lysholm scores showed no significant differences between the groups (p = 0.34, 0.301, 0.438).
Conclusions
OA-ACLR can yield a firm, stable graft reconstruction and guarantee one-stage reconstruction regardless of bony tunnel conditions. This technique can contribute to enhanced knee rotational stability and may be considered a useful alternative for revision anterior cruciate ligament reconstruction.
Trade-offs and synergistic relationships of ecosystem services in the Qinling mountains, China
Authors: Zhao Yonghua , Kang Shuaizhi , Yan Jin , Huang Xin , Zhang Yang , Wang Huanyuan , Liu Jinbao 
Published: April 2023

Abstract
To accelerate the construction of the national ecological security pattern and achieve the mutual promotion of regional ecological protection and economic development, this study focuses on the Qinling Mountains as the study area. Remote sensing, meteorological, and soil data were used to assess habitat quality, soil and water conservation, water production, and water purification trade-offs in the study area from 1980 to 2018. The study also explored the synergistic relationships among different ecosystem services and their spatial and temporal changes. The main findings revealed an overall increasing trend in habitat quality from 1980 to 2018, while soil and water conservation displayed an overall decreasing trend. Water production also showed an overall decreasing trend, while the maintenance of nitrogen and phosphorus both showed a decreasing trend. The study found a weak trade-off between habitat quality and soil conservation and water production, and a strong synergistic relationship with water quality purification. The study also revealed strong synergistic relationships between soil and water conservation and water production, weaker synergistic relationships between water production and water purification, and a stronger synergistic relationship between water production and water purification.
Identification of Soybean Genes Correlated with Rhizobial Nitrogen Fixation Activity
Authors: Yano Shunichi , Saeki Yuichi 
Published: April 2023

Abstract

Background and AimsSoybean (Glycine maxL.) has the ability to establish a symbiotic relationship with rhizobia. However, little is known about the host plant genes that correlate with the nitrogen fixation activity of rhizobia.MethodsHerein, soybean seeds were inoculated with two strains of wild-type soybean rhizobia with different nitrogen fixation activities and grown in the field until the R1 stage.ResultsThereafter, the roots and nodules were subjected to RNA-Seq to detect the expression of soybean genes related to nitrogen fixation, followed by the analysis of the community structure of the soybean-nodulating rhizobia.GmNRT2.5, a high-affinity nitrate transporter gene, was correlated with nitrogen fixation activity. Real-time PCR analysis of the roots and nodules inoculated with a nod+/fix-(Δnifgenes) mutant rhizobia showed thatGmNRT2.5on chromosome 8 (Glyma.08G284000:GmNRT2.5Chr8) was especiallycorrelated with nitrogen fixation activity.ConclusionGmNRT2.5Chr8 acts as a switching strategy between nitrogen fixation and acquisition from the soil. We also found thatGmNRT2.5Chr8 is a useful host gene that acts as an indicator of nitrogen fixation activity.
Reliability and Validity of the Chinese Version of the Achievement Emotions Questionnaire for Physical Education in University Students
Authors: Tian Jianing , Liu Peifeng , Zhang Qianqian , Song Shun , An Shicheng , Yu Hongyan 
Published: April 2023

Abstract

BackgroundAchievement emotions have a significant impact on both the learning process and outcomes. However, there is currently no brief and effective questionnaire available to evaluate Chinese university students' achievement emotions in physical education courses. This study aimed to examine the reliability and validity of the Achievement Emotions for Physical Education Questionnaire (AEQ-PE) in a sample of Chinese university students, while also investigating its measurement invariance across gender and grade levels.MethodsA cluster randomization sampling method was used to select 694 first- and second-year university students in Shanghai, China for the survey. Descriptive statistics, item analysis, reliability testing, and measurement invariance testing were conducted on the full sample (n = 694). Subsequently, the full sample was randomly divided into two groups, with Sample 1 (n = 347) undergoing exploratory factor analysis (EFA), and Sample 2 (n = 347) undergoing confirmatory factor analysis (CFA) to test the structural validity, convergent validity, and discriminant validity of the Chinese version of the Achievement Emotions Questionnaire for Physical Education (AEQ-PE-C). Finally, Sample 3 (n = 45), which was retested one month later, was used to evaluate test-retest reliability.ResultsThe Chinese version of the Achievement Emotions Questionnaire for Physical Education consists of 6 dimensions and 24 items, with good item discrimination. The EFA supported a 6-factor structure model, while the CFA demonstrated good model fit indices (χ2/df = 3.086, CFI = 0.928, TLI = 0.916, RMSEA = 0.078) and good convergent and discriminant validity. The questionnaire exhibits high internal consistency reliability (0.794) and excellent test-retest reliability (0.792). Furthermore, the multi-group analysis confirms that the AEQ-PE-C questionnaire has measurement invariance across gender and grade levels.ConclusionThe Chinese version of the Achievement Emotions Questionnaire for Physical Education has good reliability and validity, as well as measurement invariance across gender and grade levels, making it an effective tool for measuring achievement emotions in physical education among Chinese university students.