bluuebunny/crossref_metadata_2025_split huggingface dataset:
Effect of packaging materials on lycopene vitamin C and water activity of dried tomato (Lycopersicon esculentum Mill.) powder during storage
Authors: Dufera Lelise Tilahun , Hofacker Werner , Esper Albert , Hensel Oliver 
Published: July 2023

AbstractIn this work, a storage study was conducted to find suitable packaging material for tomato powder storage. Experiments were laid out in a single factor completely randomized design (CRD) to study the effect of packaging materials on lycopene, vitamin C moisture content, and water activity of tomato powder; The factor (packaging materials) has three levels (low‐density polyethylene bag, polypropylene bottle, wrapped with aluminum foils, and packed in low‐density polyethylene bag) and is replicated three times. During the study, a twin layer solar tunnel dried tomato slices of var. Galilea was used. The dried tomato slices were then ground and packed (40 g each) in the packaging materials and stored at room temperature. Samples were drawn from the packages at 2‐month interval for quality analysis and SAS (version 9.2) software was used for statistical analysis. From the result, higher retention of lycopene (80.13%) and vitamin C (49.32%) and a nonsignificant increase in moisture content and water activity were observed for tomato powder packed in polypropylene bottles after 6 months of storage. For low‐density polyethylene packed samples and samples wrapped with aluminum foil and packed in a low‐density polyethylene bag, 57.06% and 60.45% lycopene retention and 42.9% and 49.23% Vitamin C retention were observed, respectively, after 6 months of storage. Considering the results found, it can be concluded that lycopene and vitamin C content of twin layer solar tunnel dried tomato powder can be preserved at ambient temperature storage by packing in a polypropylene bottle with a safe range of moisture content and water activity levels for 6 months.
Brain volumes in alcohol use disorder: Do females and males differ? A whole‐brain magnetic resonance imaging mega‐analysis
Authors: Maggioni Eleonora , Rossetti Maria G. , Allen Nicholas B. , Batalla Albert , Bellani Marcella , Chye Yann , Cousijn Janna , Goudriaan Anna E. , Hester Robert , Hutchison Kent , Li Chiang‐Shan R. , Martin‐Santos Rocio , Momenan Reza , Sinha Rajita , Schmaal Lianne , Solowij Nadia , Suo Chao , van Holst Ruth J. , Veltman Dick J. , Yücel Murat , Thompson Paul M. , Conrod Patricia , Mackey Scott , Garavan Hugh , Brambilla Paolo , Lorenzetti Valentina 
Published: July 2023

AbstractEmerging evidence suggests distinct neurobiological correlates of alcohol use disorder (AUD) between sexes, which however remain largely unexplored. This work from ENIGMA Addiction Working Group aimed to characterize the sex differences in gray matter (GM) and white matter (WM) correlates of AUD using a whole‐brain, voxel‐based, multi‐tissue mega‐analytic approach, thereby extending our recent surface‐based region of interest findings on a nearly matching sample using a complementary methodological approach. T1‐weighted magnetic resonance imaging (MRI) data from 653 people with AUD and 326 controls was analyzed using voxel‐based morphometry. The effects of group, sex, group‐by‐sex, and substance use severity in AUD on brain volumes were assessed using General Linear Models. Individuals with AUD relative to controls had lower GM volume in striatal, thalamic, cerebellar, and widespread cortical clusters. Group‐by‐sex effects were found in cerebellar GM and WM volumes, which were more affected by AUD in females than males. Smaller group‐by‐sex effects were also found in frontotemporal WM tracts, which were more affected in AUD females, and in temporo‐occipital and midcingulate GM volumes, which were more affected in AUD males. AUD females but not males showed a negative association between monthly drinks and precentral GM volume. Our results suggest that AUD is associated with both shared and distinct widespread effects on GM and WM volumes in females and males. This evidence advances our previous region of interest knowledge, supporting the usefulness of adopting an exploratory perspective and the need to include sex as a relevant moderator variable in AUD.
First‐line immunotherapy combinations for recurrent or metastatic nasopharyngeal carcinoma: An updated network meta‐analysis and cost‐effectiveness analysis
Authors: Han Jiaqi , Zeng Ni , Tian Kun , Liu Zijian , She Longjiang , Wang Zhu , He Jinlan , Chen Nianyong 
Published: July 2023

AbstractObjectivesRecently updated results of randomized clinical trials (RCTs) have confirmed that toripalimab, camrelizumab, and tislelizumab plus chemotherapy (TOGP, CAGP, and TIGP) significantly prolonged survival compared to placebo plus chemotherapy (PLGP) in the first‐line treatment for recurrent or metastatic nasopharyngeal carcinoma (R/M‐NPC). However, the high cost of immunotherapies imposes a huge financial burden on patients and health care systems.Materials and methodsRCTs estimating immunotherapies for R/M‐NPC were searched. A Bayesian network meta‐analysis (NMA) was carried out; the main outcomes were hazard ratios (HRs) of overall survival (OS) and progression‐free survival (PFS). The cost and efficacy of four first‐line therapies were evaluated using the Markov model. The main outcome in the cost‐effectiveness analysis (CEA) was incremental cost‐utility ratios (ICURs). The model robustness was assessed by one‐way, three‐way, and probabilistic sensitivity analyses.ResultsThree RCTs (JUPITER‐02, CAPTAIN‐1st, and RATIONALE‐309) involving 815 patients were included in the NMA. Compared with PLGP, chemo‐immunotherapies have significantly longer PFS and OS. Compared to the PLGP group, TOGP, CAGP, and TIGP groups resulted in additional costs of $48 339, $22 900, and $23 162, with additional 1.89, 0.73, and 0.960 QALYs, respectively, leading to the ICURs of $25 576/QALY, $31 370/QALY, and $31 729/QALY. Pairwise comparisons showed TOGP was the most cost‐effective option among chemo‐immunotherapy groups.ConclusionFrom the Chinese payers' perspective, first‐line immunotherapy combination therapies provided significant survival and cost‐effectiveness superiority over chemotherapy alone for patients with R/M‐NPC at the WTP of $38 029/QALY. Among the three chemo‐immunotherapy groups, TOGP was the most cost‐effective option.
The efficacy of biodiversity and ecosystem assessment approaches for informing a regenerative approach to built development
Authors: Cianchi Ben , Everard Mark , Gething Bill , Cooke Rob , Ginepro Martino 
Published: July 2023

Abstract
The built environment, even at its “greenest,” inevitably entails changing ecosystem structure and function. Multiple sustainable development tools and approaches are available to reduce environmental harm from built development. However, the reality that society exists within fully integrated socioecological systems, wholly interdependent on supporting ecosystems, is not yet adequately represented in regulation or supporting tools. Regenerative development seeks to address this interdependence in part by improving the health of supporting socioecological systems through the development process. We demonstrate the relevance of a series of approaches—Local Nature-Related Planning Policy (LNRPP), Biodiversity Net Gain (BNG), the Environmental Benefits from Nature Tool (EBN Tool), Nature Assessment Tool for Urban and Rural Environments (NATURE Tool), and Rapid Assessment of Wetland Ecosystem Services+ (RAWES+)—for their ability to meet their stated aims and objectives and how these relate to wider regenerative themes. A comparative analysis of the five approaches is done by applying them to a practical case study site, resulting in policy- and practice-relevant learning and recommendations. The research reveals current gaps in methodology, which can lead to adverse outcomes for sustainability. This is particularly clear for the spatial and temporal scales across which each approach operates. In addition, this research discusses the inherent limitations of taking a reductionist approach to examining complex systems. Integr Environ Assess Manag 2024;20:248–262. © 2023 The Authors. Integrated Environmental Assessment and Management published by Wiley Periodicals LLC on behalf of Society of Environmental Toxicology & Chemistry (SETAC).
Synoviolin alleviates GSDMD‐mediated periodontitis by suppressing its stability
Authors: Pang Ying , Liu Lili , Wu Shuainan , Wang Jianqi , Liu Lu 
Published: July 2023

AbstractIntroductionInflammasome and pyroptosis play important roles in periodontitis. Gasdermin D (GSDMD), a key factor in pyroptosis, is cleaved by caspase‐1 and regulated by ubiquitination. Synoviolin is a ubiquitin E3 ligase that interacts with GSDMD. In this study, the effects of Synoviolin on inflammasome activation and periodontitis were explored.MethodsThe expression of IL‐1β, GSDMD, and Synoviolin in peripheral blood mononuclear cells from patients with periodontitis was determined. The interaction between GSDMD and Synoviolin was studied. The cytokine level in gingival tissues and the distance from the cementoenamel junction to the alveolar bone crest were measured in mice with Synoviolin deficiency in myeloid cells.ResultsWe reported that elevated mRNA and protein levels of IL‐1β and GSDMD, decreased levels of Synoviolin mRNA and protein, and decreased ubiquitination of GSDMD were associated with periodontitis. Synoviolin interacted with GSDMD. Synoviolin‐deficient bone marrow‐derived macrophages had increased IL‐1β and IL‐18 secretion after ATP stimulation. Mice with Synoviolin deficiency in myeloid cells had more severe periodontitis and upregulated IL‐1β and IL‐18.ConclusionsTherefore, we conclude that Synoviolin suppresses inflammasome activation and periodontitis by regulating GSDMD stability.
PABPC1 promotes cell proliferation and metastasis in pancreatic adenocarcinoma by regulating COL12A1 expression
Authors: Yao Weijie , Yao Yanrong , He Wen , Zhao Chengsi , Liu Di , Wang Genwang , Wang Zuozheng 
Published: July 2023

AbstractBackgroundThe expression of cytoplasmic poly (A) binding protein‐1 (PABPC1) has been reported in multiple cancer types. This protein is known to modulate cancer progression. However, the effects of PABPC1 expression in pancreatic adenocarcinoma (PAAD) have not been investigated. Here, we investigate the regulatory targets and molecular mechanisms of PABPC1 in PAAD.MethodsPABPC1 and collagen type XII α1 chain (COL12A1) expression in PAAD and their role in tumor prognosis and tumor stage were investigated using The Cancer Genome Atlas database analysis. After silencing PABPC1, messenger RNA sequencing and Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses were performed. The expression of differentially expressed genes (DEGs), cell viability, apoptosis, and cell migration and invasion were explored using reverse transcription‐quantitative polymerase chain reaction, Cell Counting Kit‐8 assay, flow cytometry assay, and transwell assay, respectively. The relationship between PABPC1 and COL12A1 expression was assessed by Pearson's correlation analysis. The regulatory function of COL12A1 in PABPC1‐affected BXPC3 cell behavior was studied after COL12A1 was overexpressed.ResultsPABPC1 and COL12A1 expression was upregulated in patients with PAAD and was linked to poor prognosis. Four hundred and seventy‐four DEGs were observed in BXPC3 cells after PABPC1 silencing. GO and KEGG analyses revealed that the top 10 DEGs were enriched in cell adhesion pathways. Additionally, PABPC1 silencing inhibited cell viability, migration, and invasion and accelerated apoptosis in BXPC3 cells. PABPC1 silencing increased AZGP1 and ARHGAP30 expression and decreased CAV1 and COL12A1 expression in BXPC3 cells. PABPC1 positively mediated COL12A1 expression, whereas PABPC1 knockdown induced the inhibition of BXPC3 cell proliferation, migration, and invasion.ConclusionThe results of this study indicate that PABPC1 may function as a tumor promoter in PAAD, accelerating BXPC3 cell proliferation and metastasis by regulating COL12A1 expression.
Two cases of Kawasaki disease: Clinical characteristics and onset of fever and gastrointestinal symptoms
Authors: Liu Hui‐Qiong , Shi Xiao‐Ya , Jia Pei‐Sheng , Huo Yu‐Feng , Mu Man‐Man , Xie Lei , Wang Huai‐Li 
Published: July 2023

AbstractBackgroundKawasaki disease (KD) is a prevalent form of systemic vasculitis that can damage various organs and systems in children. Typical KD is not difficult to diagnose in clinical practice. In recent years, it has been shown that an increasing number of children do not satisfy the diagnostic criteria for typical KD. This condition is known as incomplete KD (IKD). It is challenging to promptly diagnose and treat such children in clinical practice.Case DescriptionA 10‐year‐old girl was admitted to the hospital due to fever and abdominal pain. She presented with shock symptoms. An enhanced abdominal computed tomography scan revealed intestinal pneumatosis, effusion, and gallbladder enlargement, indicating intestinal obstruction. Due to the poor outcome following an emergency laparoscopic cholecystectomy, IKD was suspected. A 3‐month‐old male pediatric patient was admitted to the hospital due to a fever. Patchy, congestive rashes formed on the patient's body as KD progressed. IKD was suspected based on the clinical signs of fever, rash, and hyperemia of the lips. The two patients were then treated with human immunoglobulin and aspirin treatment. The prognosis for the two children was favorable following prompt treatment.ConclusionDue to the fact that IKD is frequently misdiagnosed, it is vital to (1) improve the patient prognosis for the early identification of children with KD with prolonged fever and anti‐infection failure as the initial manifestation and (2) perform timely diagnosis and comprehensive treatment.
An iASPP‐derived short peptide restores p53‐mediated cell death in cancers with wild‐type p53
Authors: Qiu Shi , Qi Wei , Wu Wen , Qiu Qian , Ma Jiali , Li Yingjun , Fan Wenhui , Li Junli , Xu Yang , Chen Hai , Liu Jie 
Published: July 2023

AbstractBackgroundInhibitor of apoptosis‐stimulating protein of p53 (iASPP) is an evolutionarily conserved p53 inhibitor. Mechanistically, iASPP can accelerate tumorigenesis by inhibiting the transactivation function of p53. Targeting the interaction between iASPP and p53 may be a potential therapy for restoring the activity of p53 in tumors.MethodsWe constructed an iASPP‐derived peptide, called A8, that was derived from the C‐terminus of iASPP. Here, we transfected A8 into two wild‐type (WT) p53 cell lines, U2OS and A549, and then determined the number of apoptotic cells. The mechanism by which A8 affected apoptosis was further examined by immunoprecipitation (IP), Dual‐Luciferase reporter assays, and chromatin IP assays. Real‐time polymerase chain reaction and western blots were also used to examine the expression levels of apoptosis‐related factors.ResultsOur data demonstrate that A8 can increase apoptosis rates in WT p53 cell lines. Functional analysis suggested that A8 restored the transcriptional function and DNA binding activities of p53 toward the Bax and PUMA gene promoters. Moreover, A8 reduced cell proliferation and inhibited tumor growth in xenograft nude mice.ConclusionsThese data provide a new approach for restoring the tumor suppressor function of p53 in cancer cells that express WT p53 and therefore may serve as a novel cancer treatment strategy.
An efficient skin cancer detection and classification using Improved Adaboost Aphid–Ant Mutualism model
Authors: Renith G. , Senthilselvi A. 
Published: July 2023

AbstractSkin cancer is the most common deadly disease caused due to abnormal and uncontrolled growth of cells in the human body. According to a report, annually nearly one million people are affected by skin cancer worldwide. To protect human lives from such life‐threatening diseases, early identification of skin cancer is the only precautionary measure. In recent times, there already exist numerous automated techniques to detect and classify skin lesion malignancies using dermoscopic images. However, analyzing the dermoscopic images becomes an arduous task due to the presence of troublesome features such as light reflections, illumination variations, and uneven shape and dimension. To address the challenges faced during skin cancer recognition process, in this paper, we proposed an efficient intelligent automated system to detect and discriminate the dermoscopic images into malignant or benign. The proposed skin cancer detection model utilizes the HAM10000 dataset for evaluation. The dermoscopic images acquired from the HAM10000 dataset are initially preprocessed to enhance the quality of image and thus making it fit to train the classifier. Afterward, the most significant image patterns are extracted by the AlexNet architecture without any loss of detailed information. Later on, the extracted features are inputted to the proposed Improved Adaboost‐based Aphid–Ant Mutualism (IAB‐AAM) classification model to discriminate the images into malignant and benign categories. The proposed IAB‐AAM approach witnessed extensive enhancement in classification accuracy. The enhanced performance is attributed by integrating the AAM optimization concept with the IAB model. By comparing the performance of the proposed IAB‐AAM with other modern methods in terms of different evaluation indicators namely accuracy, precision, specificity, sensitivity, and f‐measure, the efficiency of the proposed IAB‐AAM technique is analyzed. From the experimental results, it is known that the proposed IAB‐AAM technique attains a greater accuracy rate of 95.7% in detecting skin cancer classes than other compared approaches.
Loss of miR‐6844 alters stemness/self‐renewal and cancer hallmark(s) markers through CD44‐JAK2‐STAT3 signaling axis in breast cancer stem‐like cells
Authors: Prajapati Kumari Sunita , Kumar Shashank 
Published: July 2023

AbstractMicroRNAs regulate breast stemness and self‐renewal properties in breast cancer cells at the molecular level. Recently we reported the clinical relevance and in vitro expression profile of novel miR‐6844 in breast cancer and ‐derived stem‐like cells (mammosphere). In the present study, we first time explore the functional role of loss of miR‐6844 in breast cancer cells derived mammosphere. Down expression of miR‐6844 significantly decreased cell proliferation in MCF‐7 and T47D cells derived mammosphere in a time‐dependent manner. MiR‐6844 down expression reduced the sphere formation in terms of size and number in test cells. Loss of miR‐6844 significantly altered stemness and self‐renewal markers (Bmi‐1, Nanog, c‐Myc, Sox2, and CD44) in mammosphere compared to negative control spheres. Moreover, loss of miR‐6844 inhibits the JAK2‐STAT3 signaling pathway by decreasing p‐JAK2 and p‐STAT3 levels in breast cancer cells derived mammosphere. Loss of miR‐6844 expression significantly decreased CCND1 and CDK4 mRNA/protein levels and arrested breast cancer stem‐like cells in G2/M phase. Reduced expression of miR‐6844 increased Bax/Bcl‐2 ratio, late apoptotic cell population, and Caspase 9 and 3/7 activity in the mammosphere. Low expression of miR‐6844 decreased migratory and invasive cells by altering the expression of Snail, E‐cad, and Vimentin at mRNA/protein levels. In conclusion, loss of miR‐6844 decreases stemness/self‐renewal and other cancer hallmark in breast cancer stem‐like cells through CD44‐JAK2‐STAT3 axis. Thus, downregulation of miR‐6844 by therapeutic agents might be a novel strategy to target breast cancer stemness and self‐renewal.
Perceived social support attenuates increased hostile reactions to traumatic threat
Authors: Napolitano S. C. , Balling C. E. , Peckinpaugh I. , Samuel D. B. , Lane S. P. 
Published: July 2023

AbstractObjectiveExtant literature has seldom examined the naturalistic role of reaction to threat on downstream emotional distress while also considering buffers, such as perceived social support, to acute negative mental health outcomes. The present study examined how trauma symptoms, in reaction to a global stressor, predicted increased psychological distress via elevated emotional hostility and whether perceived social support modified such effects. We predicted a priori that increased exposure to trauma would be associated with increased hostility and global psychological distress, but that this path would be attenuated by greater levels of perceived social support, as individuals who report greater support exhibit greater emotional coping.MethodsWe recruited 408 adults from a large university in the Midwestern United States to participate in a survey assessing past‐week trauma, hostility, distress, and perceived social support following the initial COVID‐19 lockdown. The survey was conducted in March 2020, directly after strict shelter‐in‐place orders were locally mandated. To test our hypotheses, we employed a moderated mediation analysis approach.ResultsResults demonstrate that higher trauma predicted increased hostility, which in turn predicted increased distress, and trauma predicted distress via hostility (an indirect effect). As hypothesized, higher perceived social support attenuated the association between trauma and hostility.ConclusionResults support a hostile emotional pathway that may increase distress in the context of increased traumatic impact; however, social support likely buffers these effects, particularly in the face of new or novel threats and stressors. Findings suggest broad application for understanding the relation between the introduction of stressors, psychological distress, and social support.
The aminopeptidase LAP3 suppression accelerates myogenic differentiation via the AKT‐TFE3 pathway in C2C12 myoblasts
Authors: Osana Shion , Kitajima Yasuo , Naoki Suzuki , Murayama Kazutaka , Takada Hiroaki , Tabuchi Ayaka , Kano Yutaka , Nagatomi Ryoichi 
Published: July 2023

AbstractSkeletal muscle maintenance depends largely on muscle stem cells (satellite cells) that supply myoblasts required for muscle regeneration and growth. The ubiquitin–proteasome system is the major intracellular protein degradation pathway. We previously reported that proteasome dysfunction in skeletal muscle significantly impairs muscle growth and development. Furthermore, the inhibition of aminopeptidase, a proteolytic enzyme that removes amino acids from the termini of peptides derived from proteasomal proteolysis, impairs the proliferation and differentiation ability of C2C12 myoblasts. However, no evidence has been reported on the role of aminopeptidases with different substrate specificities on myogenesis. In this study, therefore, we investigated whether the knockdown of aminopeptidases in differentiating C2C12 myoblasts affects myogenesis. The knockdown of the X‐prolyl aminopeptidase 1, aspartyl aminopeptidase, leucyl‐cystinyl aminopeptidase, methionyl aminopeptidase 1, methionyl aminopeptidase 2, puromycine‐sensitive aminopeptidase, and arginyl aminopeptidase like 1 gene in C2C12 myoblasts resulted in defective myogenic differentiation. Surprisingly, the knockdown of leucine aminopeptidase 3 (LAP3) in C2C12 myoblasts promoted myogenic differentiation. We also found that suppression of LAP3 expression in C2C12 myoblasts resulted in the inhibition of proteasomal proteolysis, decreased intracellular branched‐chain amino acid levels, and enhanced mTORC2‐mediated AKT phosphorylation (S473). Furthermore, phosphorylated AKT induced the translocation of TFE3 from the nucleus to the cytoplasm, promoting myogenic differentiation through increased expression of myogenin. Overall, our study highlights the association of aminopeptidases with myogenic differentiation.