bluuebunny/crossref_metadata_2025_split huggingface dataset:
Nicotinamide enhances osteoblast differentiation through the activation of mitochondrial antioxidant defense system
Authors: Ryoo Hyun-Mo , Yoon Heein , Park Seung Gwa , Kim Hyun-Jung , Shin Hye-Lim , Kim Woo-Jin , Kim Ki-Tae , Cho Young-Dan , Moon Jae-I , Park Min-Sang 
Published: February 2023

Abstract
Though the normal physiological level of oxidative stress is beneficial for maintaining bone homeostasis, however, the imbalance between reactive oxygen species (ROS) production and antioxidant defense can cause various bone diseases. The purpose of this study was to see whether nicotinamide (NAM), an NAD+ precursor, could support the maintenance of bone homeostasis via regulating osteoblasts. Here, we demonstrate that NAM enhanced osteoblast differentiation and mitochondrial metabolism. NAM increased the expression of antioxidant enzymes, which was due to increased FOXO3a transcriptional activity via SIRT3 activation. NAM has not only a preventive effect to a weak and chronic oxidative stress but also a therapeutic effect to a strong and acute exposure to H2O2 in osteoblasts differentiation. Collectively, NAM increased mitochondrial biogenesis and antioxidant enzyme expression through the activation of SIRT3-FOXO3a axis that consequently enhanced osteoblast differentiation. These results suggest NAM could be a potential preventive or therapeutic agent for the bone diseases caused by ROS.
Unexpected Impairment of INa current Underpins Reentrant Arrhythmias in a Knock-in Swine Model of Timothy Syndrome
Authors: Porta-Sanchez Andreu , Mazzanti Andrea , Tarifa-Lora Carmen , Kukavica Deni , Trancuccio Alessandro , Mohsin Muhammad , Zanfrini Elisa , Perota Andrea , Duchi Roberto , Hernandez-Lopez Kevin , Fernandez-Moya Eugenio , Bongianino Rossana , Tavazzani Elisa , Scacchi Simone , Pavarino Luca , Franzone Piero Colli , Lentini Giovanni , Filgueiras-Rama David , Galli Cesare , Santiago Demetrio , Priori Silvia 
Published: February 2023

Abstract
Timothy Syndrome 1 (TS1) is a multiorgan form of Long QT Syndrome associated with life-threatening cardiac arrhythmias, the organ-level dynamics of which remain unclear. Here, we developed and characterized a novel porcine model of TS1 carrying the causative p.Gly406Arg mutation in CACNA1C, known to impair CaV1.2 channel inactivation. Our model fully recapitulated the human disease with prolonged QT interval and arrhythmic mortality. Electroanatomical mapping revealed the presence of a functional substrate vulnerable to reentry, stemming from an unforeseen constitutional slowing of cardiac activation. This signature substrate of TS1 was reliably identified using reentry vulnerability index, which we further demonstrate can be used as a benchmark for predicting treatment efficacy, as shown by pharmacological shortening of action potential using HERG channel agonist ICA-105574. Notably, in vitro experiments showed that TS1 cardiomyocytes display Ca2+ overload and decreased peak INa current, providing a rationale for the arrhythmogenic slowing of impulse propagation in vivo.
The telephone nursing dialogue process: an integrative review
Authors: Gustafsson Silje Rysst , Wahlberg Anna Carin 
Published: February 2023

Abstract
Background: Telephone nursing involves triage, advice, and care management provided by a nurse over the telephone. The telephone nursing dialogue process has been used clinically in telephone nursing in Sweden for several years to structure the communication and ensure a safe assessment and advice. Studies are needed to determine whether there is sufficient scientific evidence to support the method.
Aim: To describe the scientific basis of the phases of the telephone nursing dialogue process.
Design: This was an integrative review.
Methods: The literature searches were performed in March 2021, in the PubMed, CINAHL, Cochrane Database of Systematic Reviews and SwePUB databases. Fifty-five articles were included. Data was sorted deductively according to the five phases of the telephone nursing dialogue process and categorized inductively to form subcategories describing the content of each phase.
Result: All five phases in the telephone nursing dialogue process were supported by a range of articles (n=26–43): Opening (n= 26), Listening (n= 39), Analysing (n= 43), Motivating (n= 42), and Ending (n= 31). During the opening of the call, the nurse presents herself, welcomes the caller and establishes a caring relationship. In the listening phase, the nurse invites the caller to tell their story, listens actively and confirms understanding. During the analyzing phase, the nurse gathers, assesses, and verifies information. In the motivating phase, the nurse reaches a final assessment, informs the caller, gives advice and creates a mutual agreement and understanding while supporting the caller. Ultimately, the nurse ends the call after checking for mutual agreement and understanding, giving safety-net advice, deciding on whether to keep monitoring the caller and rounding off the call.
Conclusion: The phases of the telephone nursing dialogue process as described in the scientific literature are well aligned with the theoretical descriptions of the telephone nursing dialogue process.
Exploring variation in implementation of multifactorial falls risk assessment and tailored interventions: A realist review
Authors: Alvarado Natasha , McVey Lynn , Wright Judy , Healey Frances , Dowding Dawn , Cheong V-Lin , Gardner Peter , Hardiker Nick , Lynch Alison , Zaman Hadar , Smith Heather , Randell Rebecca 
Published: February 2023

Abstract
Background
Falls are the most common safety incident reported by acute hospitals. In England national guidance recommends delivery of a multifactorial falls risk assessment (MFRA) and interventions tailored to address individual falls risk factors. However, there is variation in how these practices are implemented. This study aimed to explore the variation by examining what supports or constrains delivery of MFRAs and tailored interventions.
Methods
A realist review of literature was conducted with searches completed in three stages: (1) to construct hypotheses in the form of Context, Mechanism, Outcome configurations (CMOc) about how MFRAs and interventions are delivered, (2) to scope the breadth and depth of evidence available in Embase to test the CMOc’s, and (3) following prioritisation of CMOc’s, to refine search strategies for use in multiple databases. Citations were managed in EndNote; titles, abstracts, and full texts were screened, with 10% independently screened by two reviewers.
Results
Two CMOcs were prioritised for testing labelled: Facilitation via MFRA tools, and Patient Participation in interventions. Analysis indicated that MFRA tools can prompt action, but the number and type of falls risk factors included in tools differ across organisations leading to variation in practice. Furthermore, the extent to which tools work as prompts is influenced by complex ward conditions such as changes in patient condition, bed swaps, and availability of falls prevention interventions. Patient participation in falls prevention interventions is more likely where patient directed messaging takes individual circumstances into account, e.g., not wanting to disturb nurses by using the call bell. However, interactions that elicit individual circumstances can be resource intensive and patients with cognitive impairment may not be able to participate despite appropriately directed messaging.
Conclusions
Organisations should consider how tools can be developed in ways that better support consistent and comprehensive identification of patients’ individual falls risk factors and the complex ward conditions that can disrupt how tools work as facilitators. Ward staff should be supported to deliver patient directed messaging that is informed by their individual circumstances to encourage participation in falls prevention interventions, where appropriate.
Trial registration:
PROSPERO: CRD42020184458
A Comparison of the Effects of Ticagrelor and Clopidogrel in Patients with Acute ST-Segment Elevation Myocardial Infarction: A systematic review and Meta-analysis
Authors: Geravandi Mehdi , Nourabi Mohammad , Navabifar Sepehr , Dolatshahi Sina , Zand Sara , Hooshanginezhad Zahra 
Published: February 2023

Abstract
Background
Rupture of unstable coronary atherosclerotic plaque leads to acute ST segment elevation myocardial infarction which is the most critical type of acute coronary syndrome. Dual anti-platelet therapy is one the main treatments and the combination of Aspirin and Clopidogrel is recognized as the standard oral regimen in most cases. Ticagrelor is a new generation of P2Y12 receptor inhibitors, which is a direct P2Y12 receptor antagonist. The goal of this study is to compare the effect of Ticagrelor and Clopidogrel in treatment of STEMI.
Methods
In this study, Pub Med, Scopus, Google Scholar Web of Science, Embase and Cochrane library clinical trials.gov databases were investigated. Inhomogeneity between studies was assessed using the I2 index and the Q statistic. The random effects model was used to combine studies. The Funnel plot and Egger's test were used to assess the publication bias. A probability value of less than 5 percent was considered a significant level.
Results
Eleven studies were included in this meta-analysis. Five thousand two hundred seventy-four patients in the Ticagrelor group and 5,295 patients in the Clopidogrel group were examined. The mean (standard deviation) age of the patients was 58.84 years (2.70) and 59.92 years (3.19) in the Ticagrelor group and the Clopidogrel group, respectively. Based on the results of the meta-analysis, compared to Clopidogrel, Ticagrelor had a protective effect on the outcomes of recurrent myocardial infarction, stroke, Major Adverse Cardiovascular Events (MACE), post-myocardial infarction bleeding according to Bleeding Academic Research Consortium (BARC) criteria, mortality, and reperfusion state regarding thrombolysis in myocardial infarction (TIMI) Flow Grading system. However, this effect was not statistically significant, and the publication bias was not statistically significant either.
Conclusions
According to the present study, although Ticagrelor increased the chance of bleeding according to the BARC score, compared to Clopidogrel, there was more improvement in TIMI score and fewer problems related to stroke, mortality, MI, and MACE in patients who took Ticagrelor.
Diagnosis of Felty Syndrome prior to onset of Rheumatoid Arthritis
Authors: Samander Rylee , Moore Timothy 
Published: February 2023

Abstract
Felty Syndrome is the triad of rheumatoid arthritis, neutropenia, and splenomegaly. The cause of Felty Syndrome is unknown, but neutropenia is thought to be due to a disruption in the balance between granulocyte production and removal. There is splenomegaly due to sequestration of faulty blood cells, fostering an extra-hematopoietic site. Felty Syndrome usually presents many years after diagnosis of rheumatoid arthritis and affects 1–2% of rheumatoid arthritis cases. There have been only 2 documented cases of Felty Syndrome occurring prior to the onset of rheumatoid arthritis making it a rare presentation but should be on the differential diagnosis. The medical management of Felty Syndrome includes treating the underlying cause of Rheumatoid Arthritis with systemic steroids and disease modifying anti-rheumatic drugs (DMARDs) such as methotrexate. For Felty syndrome that is refractory to medical therapy as in this case, splenectomy is usually successful. In this case presentation, this patient presented with splenomegaly and neutropenia but had not had a diagnosis of rheumatoid arthritis. The patient later tested positive for anti-CCP antibody, confirming a diagnosis of rheumatoid arthritis. Days after initial admission, the patient had onset of wrist swelling, likely from the rheumatoid arthritis. This case was complex and multiple therapies were trialed, but eventually the patient underwent a splenectomy. In conclusion, Felty Syndrome, although rare in patients with years of rheumatoid arthritis, should be considered in patients who present with splenomegaly and neutropenia and do not have a known diagnosis of rheumatoid arthritis. If caught and treated early, splenectomy may be avoided and the joint damage from rheumatoid arthritis halted.
Introduction to widely used regression models in medical research using R, STATA, and SPSS : A tutorial
Authors: Loeloe Mohammad Sadegh , Madadizadeh Farzan 
Published: February 2023

Abstract
Background: Regression models are often used to discover the relationship between variables. According to the type and scale of response variables, there are different type of regression models. Determining the type of regression is difficult for medical researchers.
Methods: In this tutorial, How to use different regressions models (ie, linear Regression, Ridge Regression, Polynomial Regression, Lasso Regression, Bayesian Linear Regression, Principal Components Regression, Partial Least Squares Regression, Elastic Net Regression, Support Vector Regression, Logistic Regression, Quantile Regression, Ordinal Regression, Poisson regression, Negative binomial regression, Quasi Poisson Regression, Fractional Regression, Cox Regression, Tobit Regression ), their computational difficulties and their assumptions are shown. The application of each regression model is also specified with a medical example, then the model is implemented in different software (ie, SPSS, STATA, R) and its output is described.
Results: The models in this study are introduced according to the dependent variable’s type and scale; some models were used to increase the efficiency and improve the estimation of the relationship between specific types of variables.
Conclusion: Today, the use of regression models is growing and new statistical methods such as data mining basically use regression as a tool for forecasting. In this tutorial, we tried to help researchers to gain a better understanding of the computational implementation of different regression models by introducing widely used regression models, providing software instructions and interpretation.
Oral Pathology and Overweight among Pupils in Government Primary Schools in Cameroon: A Cross-sectional study
Authors: BOUKENG LIONEL BERTHOLD KEUBOU , MINKANDI CLAUDE AXEL , DAPI LEONIE NZEFA 
Published: February 2023

Abstract
Background
Tooth decay and periodontal diseases are the main oral pathologies in the world. In Cameroon, a prevalence of 43% and 75% for tooth decay and periodontal disease respectively was reported in 2015. Studies shown that the prevalence of overweight was between 12.5% and 18.8% among pupils in Cameroon. These diseases share common factors associated with their burden and the consequence is the resurgence of their complications. In Cameroon, primary school enrolment rate for children aged 6 to 11 years is 93.5% and the demographic burden of pupils represent an important target. The aim of this study was to assess the relationship between oral pathologies and overweight in pupils of primary schools of Cameroon.
Methods
A cross-sectional study was carried out from June to August 2020 in four government primary schools selected through cluster sampling in Yaounde, capital city of Cameroon. Up to 650 pupils aged between 6 and 11 years whose parents gave their approval were enrolled in this study. Data collected included anthropometric, oral pathologies, quality of oral hygiene and feeding habits. The anthropometric and socio-demographic data of their mothers was also collected. Data was analyzed with the SPSS 26.0 statistical software and binary logistic regression was used to determine the risks of oral pathologies in overweight pupils. P-value of 0.05 was considered statistically significant.
Results
Out of 650 pupils sampled, 352 were girls (54.2%) and 298 boys (45.8%). The prevalence of overweight was 27% (95% CI: 23.5–30.5). The main oral pathologies were tooth decay (60.3%), gingivitis (33.1%) and periodontitis (20.2%). Binary logistic regression revealed that overweight pupils were significantly 1.5 times more likely to develop tooth decay than non-overweight pupils (95% CI: 1.1–2.4).
Conclusion
Overweight, tooth decay, gingivitis and periodontitis are prevalent among pupils in Cameroon. Overweight pupils have a higher risk of developing tooth decay compared to non-obese pupils. An integrated package of oral and nutritional health promotion activities is needed in primary schools in Cameroon.
MiR-183-5p Promotes Migration and Invasion of Prostate Cancer by Targeting TET1
Authors: Feng Yuehua , Wang Kai , Qin Minchao , Zhuang Qianfeng , Chen Zhen 
Published: February 2023

Abstract

Background:Prostate cancer (PCa) is one of the common malignant tumors and it is difficult to diagnose at its early stage. MiR-183-5p has been reported involved in the proliferation of human PCa, this study aimed to investigate how miR-183-5p affects the migration and invasion of prostate cancer.Methods:In this study, we analyzed the expression of miR-183-5p in PCa patients and its correlation with clinicopathological parameters based on TCGA data portal. CCK-8, migration assay and invasion and wound-healing assay were performed to detect proliferation, migration and invasion in PCa cells.Results:We found the expression of miR-183-5p was significantly increased in PCa tissues, and high expression of miR-183 was positively associated with poor prognosis of PCa patients. Over-expression of miR-183-5p promoted the migration, invasion capacities of PCa cells, whereas knockdown of miR-183-5p showed reversed function. Furthermore, luciferase reporter assay showed TET1 was identified as a direct target of miR-183-5p, which was negatively correlation with miR-183-5p expression level. Importantly, rescue experiments demonstrated TET1 over-expression could reverse miR-183-5p mimic induced-acceleration of PCa malignant progression.Conclusion:Our results indicated that miR-183-5p could act as a tumor promoter in PCa and it accelerated the malignant progression of PCa by directly targeting and down-regulating TET1.
Bioinformatic analysis and in vitro experiments reveal that N-linked glycosylation related genes STT3A, DDOST and TMEM165 participate in the promotion of hepatocellular carcinoma and Its role in immunotherapy
Authors: Lin Shu-sheng , Zhu Ke , Yang Cai-ni , Zhou Zi-yu , Qin Chao , Liu Chao-qun , Zhang Hong-hua , Zhang Rui , Zhu Xiang ping 
Published: February 2023

Abstract
Background: The complex tumor microenvironment of hepatocellular carcinoma (HCC) has led to a low response to immune checkpoints inhibitors (ICIs) and a poor prognosis. PD-L1, as one of the indications for ICIs, is rich in glycosylation modifications, which result in untimely ICIs. Our study constructed a prognostic model based on N-linked glycosylation related genes for predicting the prognosis and the response to ICIs.
Methods: The patients in The Cancer Genome Atlas (TCGA) and Gene Expression Omnibus (GEO) cohorts were enrolled. The Cox regression was performed to develop a prognostic model and patients were divided into a low-and high-risk subgroup. 21 recurrent HCC patients who received postoperative adjuvant ICIs were recruited and evaluated the response to ICIs. siRNA was used for in vitro validation.
Results: 59 N-linked glycosylation related differentially expressed genes were screened from of HCC and normal tissues in the TCGA cohort. The prognostic model was developed with DDOST, STT3A and TMEM165. The risk score could be an independent prognostic factor. Patients in high-risk subgroup showed worse prognosis than patients in low-risk. ssGSEA showed that patients in the low-risk subgroup were more tend to be immune-activated state. Immunohistochemistry studies showed that DDOST, STT3A and TMEM165 are highly expressed in tumor tissues and patients with high risk score correlated with poor progression free survival and worse immunotherapeutic response. Knockdown of the three genes significantly reduce the proliferation of HCC cell lines.
Conclusion: In this study, we establish that the risk model based on N-linked glycosylation related genes could efficiently predict the prognosis and tumor microenvironment immune state of HCC patients, and the risk score could serve as an indicator of ICIs.
Perception of Gratitude During a Mindfulness-Based Intervention in Patients with Interstitial Lung Disease
Authors: Miranda Siliana Maria Duarte , Pires Gabriela Patrus Ananias de Assis , MANCUZO ELIANE , Leal Andrew Nogueira Albuquerque , Paiva Sara Pinho Cunha 
Published: February 2023

Abstract

Background: Interstitial lung diseases (ILDs), regardless of their etiology, have an impact on patient quality of life at some point. Dyspnea, which is the most common symptom, cannot be treated with the main drugs available. Alternatives such as pulmonary rehabilitation, oxygen supplementation, yoga, and mindfulness practices can help in the care of patients with ILD.
Objective: To explore the perceptions of gratitude by patients with various ILDs during a mindfulness-based intervention.
Methodology: This was a qualitative study involving 50 patients with various ILDs. Data were collected through transcription of expressions of gratitude during weekly sessions. For the qualitative evaluation of the data, a six-phase thematic analysis was used.
Results: The results obtained allowed the construction of five thematic categories of gratitude: recognition of the existence of a God, religious beliefs being evident in the statements of several patients, who relied on it as a strategy for coping with the disease; the possibility of autonomy, perceived in the importance placed upon being able to perform simple, routine tasks; the presence of family and friends; the assurance of treatment by the health care team and the treatment itself; and the changes achieved through the mindfulness training.
Conclusion: Despite the severity and limitations imposed by the disease, the participants were able to perceive the importance and meaning of the presence of family and friends, spirituality, the medical team, and mindfulness in their lives.
Genetic diversity and phylogenetic analysis of Robinia pseudoacacia L. populations using ISSR Markers, ITS1 and trnL-F Intergenic spacer sequences
Authors: Uras Mehmet Emin , Ozyigit Ibrahim Ilker , Filiz Ertugrul , Sen Ugur 
Published: February 2023

Abstract
The authors have requested that this preprint be removed from Research Square.
Long-term monitoring of immune response to recombinant lumpy skin disease virus in dairy cattle from small-household farms in western Thailand
Authors: Suwankitwat Nutthakarn , Bhakha Kultyarat , Molee Lamul , Songkasupa Tapanut , Puangjinda Kanokwan , Chamchoy Tapakorn , Arjkumpa Orapun , Nuansrichay Bundit , Srisom Smit , Pongphitcha Phitcha , Lekcharoensuk Porntippa , Arunvipas Pipat 
Published: February 2023

Abstract
Lumpy skin disease (LSD) was firstly reported in Thailand in 2021. It was known that antibody against LSD virus (LSDV) could be detected until 7 months post infection. There was a limited report on immune response of LSDV infection in Thailand where recombinant vaccine strain circulated. The aim of research was to study the duration of LSD immune response of subclinical and clinical animals after natural infection in dairy cattle as well as cell-mediated immunity (CMI). Total 66 dairy cattle from ten farms affected by LSD in central and western regions of Thailand were investigated. Blood and skin nodules were tested by molecular methods. Antibody was detected by serum neutralization test (SNT) and ELISA. CMI was evaluated by gene expressions of cytokines. LSDV genome was detected in blood and skin tissues until 75 and 77 days after symptom. Antibody was detected until at least 15 months after symptom. Subclinical animals had antibody level substantially less than in clinical animals during the studied period. IFNG and TNFa levels were increased, while IL-10 level was decreased in the infected animals compared to the controls. This study elucidated immune response in dairy cattle herd affected by the first recombinant LSDV outbreak in Thailand.