bluuebunny/crossref_metadata_2025_split huggingface dataset:
Outcomes and care priorities for older people living with frailty and advanced chronic kidney disease: a multi-professional scoping review
Authors: Hurst Helen , Young Hannah M L , Nixon Andrew C , Ormandy Paula , Brettle Alison ,   The scoping review author collaborative for outcomes and care priorities for older people with ACKD, Mooney Andrew , Winterbottom Anna , Bekker Hilary , Brown Edwina A , Murtagh Fliss E M , Da Silva-Gane Maria , Coyle David , Finnigan Rob 
Published: December 2022

Abstract
The growing older population with advanced chronic kidney disease (ACKD stages 4–5) poses a challenge for healthcare worldwide. The high prevalence of frailty and associated adverse health outcomes highlights concerns for management and interventions specific to this population. The aim was to objectively review the evidence relating to older people (≥65 years) living with frailty and ACKD. More specifically how frailty is identified, what interventions have been studied and what outcomes have been reported including outcomes important to patients, families and carers. A scoping review was undertaken following the PRISMA-Scr guidelines. Nine databases were searched and a review team of five people followed a process using defined inclusion and exclusion criteria. Data were then analysed to answer the specific questions of the review. The World Health Organization’s International Classification of Functioning Disability and Health was used to map outcomes across the domains. A total of 90 studies were included. The most reported frailty measure was the frailty phenotype. The most reported outcomes were mortality, hospitalisation and healthcare utilisation. Health-related quality of life was the most common patient-reported outcome measure. There were few intervention studies and limited evidence of patient and carer perspectives. This scoping review highlights important areas for further research in older people living with frailty and ACKD. This includes a ‘gold standard’ measure for identifying frailty, interventions and improvements in outcome measures that matter to patients (including studies that focus on carers and carer burden) and priority setting for future research.
Dynamic seasonal changes in photosynthesis systems in leaves ofAsarum tamaense, an evergreen understorey herbaceous species
Authors: Wada Naoki , Kondo Issei , Tanaka Ryouichi , Kishimoto Junko , Miyagi Atsuko , Kawai-Yamada Maki , Mizokami Yusuke , Noguchi Ko 
Published: December 2022

AbstractBackground and AimsEvergreen herbaceous species in the deciduous forest understorey maintain their photosystems in long-lived leaves under dynamic seasonal changes in light and temperature. However, in evergreen understorey herbs, it is unknown how photosynthetic electron transport acclimates to seasonal changes in forest understorey environments, and what photoprotection systems function in excess energy dissipation under high-light and low-temperature environments in winter.MethodsHere, we used Asarum tamaense, an evergreen herbaceous species in the deciduous forest understorey with a single-flush and long-lived leaves, and measured photosynthetic CO2 assimilation and electron transport in leaves throughout the year. The contents of photosynthetic proteins, pigments and primary metabolites were determined from regularly collected leaves.Key resultsBoth the rates of CO2 assimilation and electron transport under saturated light were kept low in summer, but increased in autumn and winter in A. tamaense leaves. Although the contents of photosynthetic proteins including Rubisco did not increase in autumn and winter, the proton motive force and ΔpH across the thylakoid membrane were high in summer and decreased from summer to winter to a great extent. These decreases alleviated the suppression by lumen acidification and increased the electron transport rate in winter. The content and composition of carotenoids changed seasonally, which may affect changes in non-photochemical quenching from summer to winter. Winter leaves accumulated proline and malate, which may support cold acclimation.ConclusionsIn A. tamaense leaves, the increase in photosynthetic electron transport rates in winter was not due to an increase in photosynthetic enzyme contents, but due to the activation of photosynthetic enzymes and/or release of limitation of photosynthetic electron flow. These seasonal changes in the regulation of electron transport and also the changes in several photoprotection systems should support the acclimation of photosynthetic C gain under dynamic environmental changes throughout the year.
Lipid droplet synthesis is associated with angiogenesis in mouse ovarian follicles
Authors: Ibayashi Megumi , Aizawa Ryutaro , Mitsui Junichiro , Tsukamoto Satoshi 
Published: December 2022

AbstractLipid droplets (LDs) are endoplasmic reticulum (ER)-derived organelles comprising a core of neutral lipids surrounded by a phospholipid monolayer. Lipid droplets play important roles in lipid metabolism and energy homeostasis. Mammalian ovaries have been hypothesized to use neutral lipids stored in LDs to produce the hormones and nutrients necessary for rapid follicular development; however, our understanding of LD synthesis remains incomplete. In this study, we generated transgenic reporter mice that express mCherry fused to HPos, a minimal peptide that localizes specifically to nascent LDs synthesized at the ER. With this tool for visualizing initial LD synthesis in ovaries, we found that LDs are synthesized continuously in theca cells but rarely in inner granulosa cells (Gc) during early follicular development. Administration of exogenous gonadotropin enhances LD synthesis in the Gc, suggesting that LD synthesis is hormonally regulated. In contrast, we observed copious LD synthesis in the corpus luteum, and excessive LDs accumulation in atretic follicles. Furthermore, we demonstrated that LD synthesis is synchronized with angiogenesis around the follicle and that suppressing angiogenesis caused defective LD biosynthesis in developing follicles. Overall, our study is the first to demonstrate a spatiotemporally regulated interplay between LD synthesis and neovascularization during mammalian follicular development.
Slowing down or returning to normal? Life expectancy improvements in Britain compared to five large European countries before the COVID-19 pandemic
Authors: Minton Jon , Hiam Lucinda , McKee Martin , Dorling Danny 
Published: December 2022

AbstractIntroduction or backgroundLife expectancy is an important summary measure of population health. In the absence of a significant event like war or disease outbreak, trends should, and historically have, increase over time, albeit with some fluctuations.Sources of dataData were extracted from the human mortality database for life expectancy at birth and age 65 years from 1980 to the latest available year for England & Wales, Scotland (Great Britain), France, Spain, Italy, the Netherlands and Germany.Areas of agreementIt is well established that life expectancy improvements in Great Britain have stalled in recent years, and that a similar stalling was seen in other high-income countries during the mid-2010s.Areas of controversyThe significance and causes of the slowdown in improvement in life expectancy in Britain are disputed. First, was Britain’s slowdown in progress in life expectancy in the 2010s a deviation ‘from’ earlier sustained improvements or simply returning to normal (slower) improvement rates following faster gains in the 2000s? Second, did other European countries have slowdowns comparable to that in Britain?Growing pointsLife expectancy, as a summary measure, conceals inequalities. Other measures, such as lifespan disparity, complement it in understanding changing trends. While annual fluctuations in life expectancy are expected, continued stalls should raise concern. The three British nations examined were the only ones among these European countries to experience stalling of life expectancy gains in both sexes.Areas timely for developing researchWhile it is clear that Britain is making less progress in health than similar countries, more research is needed to explain why.
A mutation in DOK7 in congenital myasthenic syndrome forms aggresome in cultured cells, and reduces DOK7 expression and MuSK phosphorylation in patient-derived iPS cells
Authors: Zhang Shaochuan , Ohkawara Bisei , Ito Mikako , Huang Zhizhou , Zhao Fei , Nakata Tomohiko , Takeuchi Tomoya , Sakurai Hidetoshi , Komaki Hirofumi , Kamon Masayoshi , Araki Toshiyuki , Ohno Kinji 
Published: December 2022

Abstract
At the neuromuscular junction, the downstream of tyrosine kinase 7 (DOK7) enhances the phosphorylation of muscle-specific kinase (MuSK) and induces clustering of acetylcholine receptors (AChRs). We identified a patient with congenital myasthenic syndrome (CMS) with two heteroallelic mutations in DOK7, c.653-1G&gt;C in intron 5 and c.190G&gt;A predicting p.G64R in the pleckstrin homology domain. iPS cells established from the patient (CMS-iPSCs) showed that c.653-1G&gt;C caused in-frame skipping of exon 6 (120 bp) and frame-shifting activation of a cryptic splice site deleting seven nucleotides in exon 6. p.G64R reduced the expression of DOK7 to 10% of wild-type DOK7, and markedly compromised AChR clustering in transfected C2C12 myotubes. p.G64R-DOK7 made insoluble aggresomes at the juxtanuclear region in transfected C2C12 myoblasts and COS7 cells, which were co-localized with molecules in the autophagosome system. A protease inhibitor MG132 reduced the soluble fraction of p.G64R-DOK7 and enhanced the aggresome formation of p.G64R-DOK7. To match the differentiation levels between patient-derived and control induced pluripotent stem cells (iPSCs), we corrected c.190G&gt;A (p.G64R) by CRISPR/Cas9 to make isogenic iPSCs while retaining c.653-1G&gt;C (CMS-iPSCsCas9). Myogenically differentiated CMS-iPSCs showed juxtanuclear aggregates of DOK7, reduced expression of endogenous DOK7 and reduced phosphorylation of endogenous MuSK. Another mutation, p.T77M, also made aggresome to a less extent compared with p.G64R in transfected COS7 cells. These results suggest that p.G64R-DOK7 makes aggresomes in cultured cells and is likely to compromise MuSK phosphorylation for AChR clustering.
RAfilter: an algorithm for detecting and filtering false-positive alignments in repetitive genomic regions
Authors: Yang Jinbao , Zhao Xianjia , Jiang Heling , Yang Yingxue , Hou Yuze , Pan Weihua 
Published: December 2022

Abstract
Telomere to telomere (T2T) assembly relies on the correctness of sequence alignments. However, the existing aligners tend to generate a high proportion of false-positive alignments in repetitive genomic regions which impedes the generation of T2T-level reference genomes for more important species. In this paper, we present an automatic algorithm called RAfilter for removing the false-positives in the outputs of existing aligners. RAfilter takes advantage of rare k-mers representing the copy-specific features to differentiate false-positive alignments from the correct ones. Considering the huge numbers of rare k-mers in large eukaryotic genomes, a series of high-performance computing techniques such as multi-threading and bit operation are used to improve the time and space efficiencies. The experimental results on tandem repeats and interspersed repeats show that RAfilter was able to filter 60%–90% false-positive HiFi alignments with almost no correct ones removed, while the sensitivities and precisions on ONT datasets were about 80% and 50% respectively.
The root meristem growth factorBrRGF6positively regulates Chinese cabbage to infection of clubroot disease caused byPlasmodiophora Brassicae
Authors: Ge Wenjie , Zhang Jing , Feng Hui , Wang Yilian , Ji Ruiqin 
Published: December 2022

AbstractChinese cabbage has a high annual demand in China. However, clubroot disease caused by the infection of Plasmodiophora brassicae seriously affects its yield. Transcriptome analysis identified a root meristem growth factor 6 (BrRGF6) as significantly up-regulated in Chinese cabbage roots infected with Plasmodiophora brassicae. Quantitative reverse-transcription polymerase chain reaction and in situ hybridization analysis showed higher BrRGF6 expression in susceptible materials than in resistant materials. After Plasmodiophora brassicae infection, BrRGF6 expression was significantly up-regulated, especially in susceptible materials. Gene function analysis showed that the roots of Arabidopsis mutant rgf6 grew faster than the wild-type, and delayed the infection progress of Plasmodiophora brassicae. A Protein, nuclear transcription factor Y subunit C (BrNF-YC), was screened from yeast two-hybrid library of Chinese cabbage induced by Plasmodiophora brassicae, and verified to interact with BrRGF6 by yeast two-hybrid co-transfer. Yeast one-hybrid and β-Glucuronidase activity analysis showed that BrNF-YC could directly bind to and strongly activate the promoter of BrRGF6. Transgenic verification showed that BrRGF6 or BrNF-YC silenced Chinese cabbage significantly decreased the expression of BrRGF6, accelerated root development, and reduced incidence of clubroot disease. However, after overexpression of BrRGF6 or BrNF-YC, the phenotype showed a reverse trend. Therefore, BrRGF6 silencing accelerated root growth and enhanced resistance to clubroot disease, which was regulated by BrNF-YC.
Correction of beam overlap-induced athwart distortion in multibeam sonars
Authors: Boyra Guillermo , Martínez Udane , Uranga Jon , Moreno Gala , Peña Héctor 
Published: December 2022

Abstract
A method is proposed to estimate and correct athwart-beam distortion of multibeam sonars to advance sonar-based abundance estimation. We illustrate its application using data from a Simrad SN90 multibeam sonar aboard a tropical tuna purse-seiner, targeting mixed fish aggregations around drifting Fish Aggregating Devices (dFADs) in the Atlantic Ocean. To calculate the distortion in the horizontal swath, athwart-beam measurements were compared with the more accurate simultaneous along-beam measurements. For the vertical swath correction, we used as reference a vertically oriented single, split beam echosounder simultaneously monitoring the same aggregations. Along-to-athwart ratios of ∼0.6 and ∼0.3 were estimated for the horizontal and vertical swaths, respectively, at ranges involved during purse-seine operations in this fleet (from ∼225 to ∼325 m). Additionally, equations were developed to analytically describe the athwart distortion due to overlap between contiguous beams. Once corrected for distortion, typical school morphology measures were provided for tuna aggregations around dFADs.
VEGFR-TKI treatment for radiation-induced brain injury after gamma knife radiosurgery for brain metastases from renal cell carcinomas
Authors: Noda Ryuichi , Akabane Atsuya , Kawashima Mariko , Uchino Keita , Tsunoda Sho , Segawa Masafumi , Inoue Tomohiro 
Published: December 2022

AbstractObjectiveAntiangiogenic vascular endothelial growth factor receptor tyrosine kinase inhibitors play an essential role in systemic therapy for renal cell carcinoma. Given the anti-edematous effect of bevacizumab, an antiangiogenic antibody targeting vascular endothelial growth factor, vascular endothelial growth factor receptor tyrosine kinase inhibitors should exert therapeutic effects on radiation-induced brain injury after stereotactic radiosurgery. This preliminary study aimed to investigate the therapeutic effect of vascular endothelial growth factor receptor tyrosine kinase inhibitor against radiation-induced brain injury.MethodsMagnetic resonance images for six patients treated with vascular endothelial growth factor receptor tyrosine kinase inhibitors who were diagnosed with radiation-induced brain injury following gamma knife radiosurgery were retrospectively reviewed.ResultsThe median brain edema volume and tumour mass volume in the pre-tyrosine kinase inhibitor period were 57.6 mL (range: 39.4–188.2) and 3.2 mL (range: 1.0–4.6), respectively. Axitinib, pazopanib (followed by cabozantinib) and sunitinib were administered in four, one and one cases, respectively. The median brain edema volume and tumour mass volume in the post-tyrosine kinase inhibitor period were 4.8 mL (range: 1.5–27.8) and 1.6 mL (range: 0.4–3.6), respectively. The median rates of reduction in brain edema volume and tumour mass volume were 90.8% (range: 51.9–97.6%) and 57.2% (range: 20.0–68.6%), respectively. The post-tyrosine kinase inhibitor values for brain edema volume (P = 0.027) and tumour mass volume (P = 0.008) were significantly lower than the pre-tyrosine kinase inhibitor values. Changes in volume were correlated with tyrosine kinase inhibitor use.ConclusionThis study is the first to demonstrate the therapeutic effects of vascular endothelial growth factor receptor tyrosine kinase inhibitors on radiation-induced brain injury in patients with brain metastases from renal cell carcinoma treated via gamma knife radiosurgery.
Modelling stellar activity with Gaussian process regression networks
Authors: Camacho J D , Faria J P , Viana P T P 
Published: December 2022

ABSTRACT
Stellar photospheric activity is known to limit the detection and characterization of extrasolar planets. In particular, the study of Earth-like planets around Sun-like stars requires data analysis methods that can accurately model the stellar activity phenomena affecting radial velocity (RV) measurements. Gaussian Process Regression Networks (GPRNs) offer a principled approach to the analysis of simultaneous time series, combining the structural properties of Bayesian neural networks with the non-parametric flexibility of Gaussian Processes. Using HARPS-N solar spectroscopic observations encompassing three years, we demonstrate that this framework is capable of jointly modelling RV data and traditional stellar activity indicators. Although we consider only the simplest GPRN configuration, we are able to describe the behaviour of solar RV data at least as accurately as previously published methods. We confirm the correlation between the RV and stellar activity time series reaches a maximum at separations of a few days, and find evidence of non-stationary behaviour in the time series, associated with an approaching solar activity minimum.
Radio jet–ISM interaction and positive radio-mechanical feedback in Abell 1795
Authors: Tamhane Prathamesh D , McNamara Brian R , Russell Helen R , Combes Francoise , Qiu Yu , Edge Alastair C , Maiolino Roberto , Fabian Andrew C , Nulsen Paul E J , Johnstone R , Carniani Stefano 
Published: December 2022

ABSTRACT
We present XSHOOTER observations with previous ALMA, MUSE, and HST observations to study the nature of radio jet triggered star formation and the interaction of radio jets with the interstellar medium in the brightest cluster galaxy (BCG) in the Abell 1795 cluster. Using HST UV data, we determined an ongoing star formation rate of 9.3 M⊙ yr−1. The star formation follows the global Kennicutt–Schmidt law; however, it has a low efficiency compared to circumnuclear starbursts in nearby galaxies with an average depletion time of ∼1 Gyr. The star formation and molecular gas are offset by ∼1 kpc indicating that stars have decoupled from the gas. We detected an arc of high linewidth in ionized gas where electron densities are elevated by a factor of ∼4 suggesting a shock front driven by radio jets or peculiar motion of the BCG. An analysis of nebular emission line flux ratios suggests that the gas is predominantly ionized by star formation with a small contribution from shocks. We also calculated the velocity structure function (VSF) of the ionized and molecular gases using velocity maps to characterize turbulent motion in the gas. The ionized gas VSF suggests that the radio jets are driving supersonic turbulence in the gas. Thus radio jets cannot only heat the atmosphere on large scales and may quench star formation on longer time-scales while triggering star formation in positive feedback on short time-scales of a few million years.
Investigation of the connection between X-ray binaries and compact star clusters in NGC 628
Authors: Avdan S , Akyuz A , Acar S , Akkaya Oralhan I , Allak S , Aksaker N 
Published: December 2022

ABSTRACTWe investigated for a possible connection between the types of X-ray binaries (XRBs) and the properties of compact star clusters in the nearby galaxy NGC 628. Using Chandra archival data covering the years 2001–2018, 75 X-ray sources were detected within the field of view of observations. A total of 69 XRBs, one of which is an ultraluminous X-ray source (ULX), were found to be in the luminosity range of 3 × 1036 ≤ LX ≤ 2 × 1039 erg s−1. We identified the optical counterpart(s) of 15 of the 42 XRBs that coincide with the HST field of view via improved astrometry. We classified 15 of them as HMXBs based on the presence of the optical counterparts. The remaining sources with no optical counterparts were classified as LMXBs. We also search compact star clusters in this galaxy using the multiband optical images drawn from HST archives. 864 compact star clusters were identified and their ages and masses were determined by applying the best-fit SSP (Simple Stellar Population) model to their colour–colour diagram. We found that in NGC 628, HMXBs are associated with younger star clusters and LMXBs with older ones. Our findings support a connection between different types of XRBs and cluster ages, already known to exist for other galaxies.
Small molecule-mediated disruption of ribosome biogenesis synergizes with FGFR inhibitors to suppress glioma cell growth
Authors: Zisi Asimina , Kanellis Dimitris C , Moussaud Simon , Karlsson Ida , Carén Helena , Bräutigam Lars , Bartek Jiri , Lindström Mikael S 
Published: December 2022

Abstract

Background
High-grade gliomas are malignant brain tumors characterized by aggressiveness and resistance to chemotherapy. Prognosis remains dismal, highlighting the need to identify novel molecular dependencies and targets. Ribosome biogenesis (RiBi), taking place in the nucleolus, represents a promising target as several cancer types rely on high RiBi rates to sustain proliferation. Publicly available transcriptomics data of glioma patients revealed a positive correlation between RiBi rates and histological grades. We, therefore, hypothesized that glioma cells could be susceptible to RiBi inhibition.

Methods
Transcriptomics data from glioma patients were analyzed for RiBi-related processes. BMH-21, a small molecule inhibitor of RNA pol I transcription, was tested in adult and pediatric high-grade glioma cell lines and a zebrafish transplant model. Cellular phenotypes were evaluated by transcriptomics, cell cycle analysis, and viability assays. A chemical synergy screen was performed to identify drugs potentiating BMH-21-mediated effects.

Results
BMH-21 reduced glioma cell viability, induced apoptosis, and impaired the growth of transplanted glioma cells in zebrafish. Combining BMH-21 with TMZ potentiated cytotoxic effects. Moreover, BMH-21 synergized with Fibroblast Growth Factor Receptor (FGFR) inhibitor (FGFRi) Erdafitinib, a top hit in the chemical synergy screen. RiBi inhibition using BMH-21, POLR1A siRNA, or Actinomycin D revealed engagement of the FGFR–FGF2 pathway. BMH-21 downregulated FGFR1 and SOX2 levels, whereas FGF2 was induced and released from the nucleolus.

Conclusions
This study conceptualizes the implementation of RiBi inhibition as a viable future therapeutic strategy for glioma and reveals an FGFR connection to the cellular response upon RiBi inhibition with potential translational value.