bluuebunny/crossref_metadata_2025_split huggingface dataset:
Establishment and characterization of a novel hilar cholangiocarcinoma cell line, CBC3T-1
Authors: Bai Mingzhen , Jiang Ningzu , Fu Wenkang , Huang Chongfei , Tian Liang , Mi Ningning , Gao Long , Ma Haidong , Lu Yawen , Cao Jie , Zhang Chao , Yue Ping , Zhang Yong , Lin Yanyan , Meng Wenbo , Li Xun 
Published: September 2023

Abstract
Cholangiocarcinoma (CCA) is a group of malignant heterogeneous cancer arising from the biliary tree. The tumor is characterized by insidious onset, high degree of malignancy, poor prognosis and high recurrence rate. Immortalized cancer cell lines are the best and easiest models for in vitro cancer research. Here, we established a naturally immortalized highly tumorigenic hilar cholangiocarcinoma (hCCA) cell line, CBC3T-1. The CBC3T-1 cell line was cultured for over 60 passages. Thorough analysis showed that CBC3T-1 cells share characteristics similar to original tumor cells from patients with cholangiocarcinoma and display a stable phenotype, including features of epithelial origin, stem cell-like properties as well as a high invasive and migratory capability and tumorigenicity in mice. Furthermore, this cell line showed the best sensitivity to paclitaxel, followed by gemcitabine. RNA sequencing and whole‑exome sequencing showed that cancer-associated pathways and somatic mutations played a dominant role in the development of CCA. We established and characterized a new hCCA cell line, CBC3T-1, which contributes to a better understanding of bile duct cancer and can be used to study tumorigenesis and progression and the role of anticancer drugs.
NMR 1H, 13C, 15N backbone resonance assignments of wild-type human K-Ras and its oncogenic mutants G12D and G12C bound to GTP
Authors: Yuan Chunhua , Hansen Alexandar L. , Bruschweiler-Li Lei , Brüschweiler Rafael 
Published: September 2023

Abstract
Human K-Ras protein, which is a member of the GTPase Ras family, hydrolyzes GTP to GDP and concomitantly converts from its active to its inactive state. It is an important oncoprotein, because several mutations, particularly those at residue position 12, occur with a high frequency in a wide range of human cancers. The K-Ras protein is therefore an important target for developing therapeutic anti-cancer agents. In this work we report the almost complete sequence-specific resonance assignments of wild-type, G12C and G12D mutants in the GTP-complexed active forms, including the functionally important Switch I and Switch II regions. These assignments serve as the basis for a comprehensive functional dynamics study of wild-type K-Ras and its G12 mutants.
Reconfiguration of four-degree limited Laman graphs
Authors: Trinh Hoang Minh 
Published: September 2023

Abstract

Several simple algorithms are proposed for expanding and reducing a Laman graph, of which each vertex has at most four neighbors. These algorithms can be iteratively applied to construct, reconfigure, or destroy a given four-degree limited Laman graph. The validity of the proposed algorithms is asserted by Laman’s criterion.MSC Classification:68R10
Correlates of preconception and pregnancy hair cortisol concentrations
Authors: Juvinao-Quintero Diana L. , Künzel Richard G. , Larabure-Torrealva Gloria , Duncan Laramie , Kirschbaum Clemens , Sanchez Sixto E. , Gelaye Bizu 
Published: September 2023

Abstract

Assessing factors that influence chronic stress biomarkers like hair cortisol concentrations (HCCs) in pregnancy is critical to prevent adverse pregnancy outcomes. Thus, we aimed to identify correlates of HCC preconception and during pregnancy. 2,581 pregnant women participated in the study. HCC was available at four time periods: pre-pregnancy (0–3 months preconception, n = 1,023), and in the first (1–12 weeks, n = 1,734), second (13–24 weeks, n = 1,534), and third (25–36 weeks, n = 835) trimesters. HCC was assessed using liquid chromatography tandem mass spectrometry (LC-MS/MS). Sociodemographic, pregnancy- and hair-related characteristics, and measures of psychosocial stress, were interrogated as potential correlates of HCC. Spearman correlations, paired t-tests, and ANOVA were used to assess differences in log-transformed values of HCC (logHCC) across maternal characteristics. Multivariable linear regressions were used to identify the correlates of HCCs after adjusting for confounders. Mean logHCC values increased across the four prenatal periods (P < 0.001). In multivariable analyses, pre-pregnancy BMI was consistently associated with all HCCs, while gestational age, economic hardship, hair dyeing, and depression, showed time-specific associations with HCC. In conclusion, this study showed evidence of factors influencing HCC levels before and during pregnancy. The most consistent association was seen with pre-pregnancy BMI. Depression was also associated with HCC concentrations.
Enhancing statistical chart accessibility for people with low vision: insights from a user test
Authors: Alcaraz-Martínez Rubén , Ribera Mireia , Adeva-Fillol Adrià , Pascual-Almenara Afra 
Published: September 2023

Abstract

Purpose
A remote user test was performed with two versions (one accessible and another one non-accessible) of three types of web-based charts (horizontal bar chart, vertical stacked bar chart, and line chart). The objectives of the test were a) to validate a set of heuristic indicators for the evaluation of the accessibility of statistical charts presented in a previous work [7]; b) to identify new barriers and preferences for users with low vision in the access and use of this content not previously contemplated.
Methods
12 users were tested, with a variety of conditions associated with low vision: low visual acuity (6 users), reduced central vision (2 users), reduced peripheral vision (2 users), blurry vision (1 user), sensitivity to light (3 users), Nystagmus (2 users) and color vision deficiency (CVD) (4 users).
Results
From a quantitative standpoint, accessible versions of charts were more efficient, effective, and satisfactory. From a qualitative point of view, results verify the relevance of heuristics H2, Legend; H3, Axes; H6, Data source (as data table); H10, Safe colors; H11, Contrast; H12, Legibility; H13, Image quality; H14, Resize; H16, Focus visible; H17, Independent navigation; related to the proposed tasks. As new observations, tooltips were highly valued by all users, but their implementation must be improved to avoid covering up significant parts of the charts when displayed. The data table has also been frequently used by all users, especially in the non-accessible versions, allowing them to carry out tasks more efficiently. The position and size of the legend can be a significant barrier if it is too small or appears in an unusual position. Finally, despite the limitations related to color perception, some users prefer color graphics to black and white, so, to target all profiles, it is necessary to redundantly encode categories with colors and patterns as well.
Clay Soil Porosity Estimation Using Seismic P and S-Wave Velocities
Authors: Chatrayi Hosein , Hajizadeh Farnusch , Taghavi Behnam 
Published: September 2023

Abstract

To study how the soils, respond to an earthquake, seismic waves are frequently utilized. The purpose of this work is to build the porosity graphs based on the geotechnical parameters of the soils and forecast the porosity of shallow clay soils using seismic wave velocities that analyze the dynamic features of the soil. Compressional (P) and shear (S) wave velocities, seismic velocity ratio, Poisson ratio, bulk modulus, and shear modulus are the factors used to calculate porosity. In this work, porosity values are calculated using grain and dry densities of core samples taken from different boreholes within the study region, and bulk modulus, shear modulus, and Poisson ratio are calculated using P- and S-wave velocities obtained by utilizing the seismic-refraction method, as well as porosity values. The research region is in Iran; Isfahan Metro Line 2 and mostly consists of clay, silt, sand, and gravel deposits. Based on the values of the Poisson ratio, seismic P wave velocity, seismic velocity ratio (Vp/Vs), and the stiffness of the clay soils, the data of the clay soils in the region were individually sorted. These characteristics were used to create novel multi-parameter relationships between clay soil porosity, seismic velocities, shear modulus, and the Poisson ratio. Using the error norm approach, the errors in the parameters utilized for each relationship were identified. The error norm technique's findings show that the shear wave velocity and shear module have the lowest error when calculating porosity. Therefore, it is advised to estimate porosity of shallow clay soils using the given correlations. These relationships can be used to assess the porosity of clay soil and to determine if the soil's pores are saturated with liquid.
Radiomics of pericoronary adipose tissue on computed tomography angiography predicts coronary heart disease in patients with type 2 diabetes mellitus
Authors: Miao Shumei , Yu Feihong , Rongrong Sheng , Zhang Xiaoliang , Li Yong , Qi Yaolei , Lu Shan , Ji Pei , Fan Jiyue , Zhang Xin , Xu Tingyu , Zhongmin Wang , Yang Guanyu , Liu Yun 
Published: September 2023

Abstract

Background
Diabetes is a common chronic metabolic disease. The progression of the disease promotes vascular inflammation and the formation of atherosclerosis, leading to cardiovascular disease. The coronary artery perivascular adipose tissue attenuation index based on CCTA is a new noninvasive imaging biomarker that reflects the spatial changes in perivascular adipose tissue attenuation in CCTA images and the inflammation around the coronary arteries. In this study, a radiomics approach is proposed to extract a large number of image features from CCTA in a high-throughput manner and combined with clinical diagnostic data to explore the predictive ability of vascular perivascular adipose imaging data based on CCTA for coronary heart disease in diabetic patients.
Methods
R language was used for statistical analysis to screen the variables with significant differences. A presegmentation model was used for CCTA vessel segmentation, and the pericoronary adipose region was screened out. PyRadiomics was used to calculate the radiomics features of pericoronary adipose tissue, and SVM, DT and RF were used to model and analyze the clinical data and radiomics data. Model performance was evaluated using indicators such as PPV, FPR, AAC, and ROC.
Results
The results indicate that there are significant differences in age, blood pressure, and some biochemical indicators between diabetes patients with and without coronary heart disease. Among 1037 calculated radiomic parameters, 18.3% showed significant differences in imaging omics features. Three modeling methods were used to analyze different combinations of clinical information, internal vascular radiomics information and pericoronary vascular fat radiomics information. The results showed that the dataset of full data had the highest ACC values under different machine learning models. The support vector machine method showed the best specificity, sensitivity, and accuracy for this dataset.
Conclusions
In this study, the clinical data and pericoronary radiomics data of CCTA were fused to predict the occurrence of coronary heart disease in diabetic patients. This provides information for the early detection of coronary heart disease in patients with diabetes and allows for timely intervention and treatment.
A Switchable Demultiplexer utilizing Magnetic-Based Phononic Crystal Configuration
Authors: Ranjbar Sajjad , Nazari Fakhroddin , Hajizadeh Rasoul 
Published: September 2023

Abstract
This study presents a novel approach to achieve a controllable demultiplexer by applying a magnetic field in a solid-solid phononic crystal structure. The proposed phononic crystal structure consists of a square lattice made of tungsten cylinders embedded in a PMMA substrate. The switchable demultiplexer is composed of two identical but symmetrical parts, with an input waveguide separating them. Each unit comprises an output channel and a common input channel, with output channels side-coupled by a ring resonator to the input bus waveguide. The operating frequency range of the demultiplexer is in the MHz regime. The switchability of this elastic demultiplexer is achieved by dynamically controlling Young's modulus. By applying a magnetic field to the structure, the Young's modulus of the Terfenol-D material used in the output waveguide is altered, allowing for controllability. The proposed demultiplexer demonstrates an average crosstalk value of -12.65 dB, indicating suitable performance, along with a high average quality factor (Q) of 1870. The solid-solid elastic structure is simulated using the finite element method.
Efficacy and Safety of Radiotherapy Plus Immunochemotherapy in Patients with Oligometastatic Esophageal Cancer
Authors: duan Yanan , cheng shuping , qin wenru , zou bing , fan bingjie , wang linlin 
Published: September 2023

Abstract
Background and purpose: Immunotherapy had revolutionized the treatment of metastatic esophageal cancer. Oligometastasis is a limited-metastatic state, and patients at this stage may benefit from metastatic-directed local treatments, which may increase their chances of survival. As an immunomodulator, radiotherapy (RT) might achieve a synergistic effect when combined with immunotherapy. Compared to immunochemotherapy alone, this study assessed the efficacy and safety of radiotherapy plus immunochemotherapy in de-novo oligometastatic esophageal cancer (EC).
Materials and methods: In this trial, we retrospectively collected esophageal cancer patients with de-novo oligometastases and received immunochemotherapy. The primary endpoints were progression-free survival (PFS) and overall survival (OS).Secondary endpoints were objective response rate (ORR), disease control rate (DCR), duration of response (DOR), and treatment-related adverse events (TREAs).
Results: Between Jan 2019 and Jan 2022, 226 patients were included, which consisted of 108 cases in the RT plus immunochemotherapy (iCRT) group, and 118 cases in the immunochemotherapy (iCT) group. With a median follow-up of 22.2 months, the median PFS (mPFS) was 13.0 months for the iCRT group and 7.7 months for the iCT group (P＜0.001, HR=0.520, 95% CI, 0.388-0.696). In terms of OS, the median OS (mOS) was 27.5 months for the iCRT group and 21.7 months for the iCT group (P=0.026, HR=0.670, 95% CI, 0.468-0.958). The iCRT group compared with the iCT group was associated with a higher risk of ≥ grade 3 myelosuppression. No other grade 5 TRAEs or new safety signals were observed. Through univariate and multivariate analysis, we also found that, besides local radiotherapy, oligometastatic esophageal cancer (OMEC) patients with a history of alcohol consumption, more metastatic lesions, and second-line and above treatment had inferior PFS. And a lower KPS score, a greater number of metastatic lesions, and second-line and above treatment were found to have inferior OS.
Conclusion: Compared to immunochemotherapy alone, radiotherapy plus immunochemotherapy showed survival benefits with manageable safety for oligometastatic esophageal cancer patients. It deserves to be validated in a larger trial.
WITHDRAWN: P-Delta Effects Assessment in Precast Structures Via Inelastic Performance Based Spectra
Authors: Nascimbene Roberto , Sisti Andrea 
Published: September 2023

Abstract

The seismic design of precast structures is significantly impacted by the inherent characteristics of precast technology. One primary concern is to deliver structural elements that are as lightweight as possible to streamline on-site assembly and cost reduction. Consequently, the slenderness of beams and columns is considerably greater compared to traditional cast-in-situ concrete constructions, and second-order effects assume a pivotal role. Furthermore, these very factors make dry pinned joints the preferred choice for designers when it comes to connecting beams and columns. Cast-in-situ concrete is typically reserved for connections between columns and foundations, as well as for topping off precast floor elements. Pinned joints result in the transformation of the frame into an ideal isostatic structure, with cantilevered columns securely anchored at the base. This transformation leads to a significant reduction in the energy dissipation capacity of the entire structure. It not only prevents the formation of plastic hinges in the beams but also amplifies the P-Delta effects in the column's response by decreasing the overall stiffness when subjected to lateral loads. A simplified approach for assessing dynamic instability in single and multi-storey precast hinged frames is presented. The objective is to create a tool for the initial design of such structures, enabling the prediction of dynamic collapse and the achievement of limit states during seismic events based on fundamental structural parameters. The impact of these parameters on the overall behaviour is explored through incremental dynamic nonlinear analysis, using real far-field accelerograms, on equivalent single-degree-of-freedom systems. The result is a set of inelastic spectra, giving the structural capacity in term of force reduction factor versus parameters like building height, column aspect ratios and floor masses configuration, with regard to different limit states usually considered in seismic design of these structures.
Risk prediction model for mortality in microscopic polyangiitis: multicentre REVEAL cohort study
Authors: Kotani Takuya , Matsuda Shogo , Okazaki Ayana , Nishioka Daisuke , Watanabe Ryu , Gon Takaho , Manabe Atsushi , Shoji Mikihito , Kadoba Keiichiro , Hiwa Ryosuke , Yamamoto Wataru , Hashimoto Motomu , Takeuchi Tohru 
Published: September 2023

Abstract
Background: To establish refined risk prediction models for mortality in patients with microscopic polyangiitis (MPA) by using comprehensive clinical characteristics.
Methods: Data from the multicentre Japanese Registry of Patients with Vasculitis (REVEAL cohort) were used in our analysis. In total, 194 patients with newly diagnosed MPA were included, and baseline demographic, clinical, laboratory, and treatment details were collected. Univariate and multivariate analyses were conducted to identify the significant risk factors predictive of mortality.
Results: Over a median follow-up of 202.5 (84–352) weeks, 60 (30.9%) of 194 patients died. The causes of death included MPA-related vasculitis (18.3%), infection (50.0%), and other (31.7%). Deceased patients were older (median age 76.2 years) than survivors (72.3 years) (P < 0.0001). The death group had shorter observation periods (median 128.5 [35.3–248] weeks) than the survivor group (229 [112–392] weeks). Compared to survivors, the death group exhibited a higher smoking index, lower serum albumin levels, higher serum C-reactive protein levels, higher Birmingham Vasculitis Activity Score (BVAS), higher Five-Factor Score, and a more severe European Vasculitis Study Group (EUVAS) categorization system. Multivariate analysis revealed that higher BVAS and severe EUVAS independently predicted mortality. Kaplan–Meier survival curves demonstrated lower survival rates for BVAS ≥20 and severe EUVAS, and a risk prediction model (RPM) based on these stratified patients into low, moderate, and high-risk mortality groups.
Conclusions: The developed RPM is promising to predict mortality in patients with MPA, and provides clinicians with a valuable tool for risk assessment and informed clinical decision-making.
Exploring Bangladeshi EFL Learners’ Mindfulness and Engagement in Online Learning Context
Authors: Khatami Mojtaba , Shayerey Afsana Jerin , Behera Santosh Kumar , He Deyuan , Haji-Othman Noor Azam 
Published: September 2023

Abstract

The study investigates the relationship between Bangladeshi English as a Foreign Language(EFL) learners’ mindfulness and their engagement in online learning context due to Covid-19. Langer Mindfulness Scale (LMS) and Online Student Engagement Scale (OSES) were employed to gather data from 215 male and female Bangladeshi university students on a voluntary basis. Descriptive and regression analyses revealed that there was no significant relationship between mindfulness and online student engagement. Thematic analysis of respondents’ interview data collected through open-ended questions revealed that more than 80% of Bangladeshi EFL students preferred in-person classes due to unreliable internet connection, stress, anxiety, and lack of interaction with teachers and peers. The study suggests that policy makers should prioritize promoting internet access and reliability to facilitate effective online classes, which could alleviate stress and anxiety among students and provide a platform for learning-friendly interactions between students and teachers.
"We're all learning together”: Educator perspectives on medical student reflective practice
Authors: Woodall Hannah , Carrigan Brendan , Furness Linda , Heffernan Robert , Pinidiyapathirage Janani , Brumpton Kay 
Published: September 2023

Abstract

Introduction
Reflective learning skills are important in the development of medical students. Through reflection, students learn about self and medicine and the integration of theoretical knowledge into real life work. However, reflection requires time, willingness, and skills on the part of the student. This study explored the factors perceived by educators to influence reflective learning amongst medical students undertaking a rural longitudinal integrated clerkship (LIC) in southeast Queensland.
Methods:
Within the described LIC program, medical students receive teaching and support from both medical and nursing educators. Two qualitative focus groups were conducted with eleven educators from a medical (6 participants) and nursing (5 participants) background. Transcripts were analysed using Braun and Clark’s approach to thematic analysis.
Results:
Three major themes were identified as contributing to student reflective learning. Fostering a culture of reflection within the organisation was seen as important for supporting and setting expectations for reflection. Undertaking the reflective learning journey together recognised the importance of students and educators learning collaboratively. Finally, looking through “the student lens” allowed educators to recognise the wider contextual factors impacting on student reflection. Recognition of these factors allows educators to tailor reflective teaching to the student.
Discussion
Reflective learning is a critical skill for medical students and their educators. This study identified that reflection can be supported through the development of a supportive organisational culture, through encouraging educators and students to learn together, and through recognising the student perspective.
Consensus of multi-agent systems with randomly occurring nonlinearities via uncertain pinning control under switching topologies *
Authors: Sui Xin , Yang Yongqing , Wang Fei 
Published: September 2023

Abstract
This paper is focused on the consensus problem of multi-agent systems via uncertain pinning control under switching topologies. The stochastic disturbances and randomly occurring nonlinearities are proposed to describe more realistic systems. The communication topology is modeled by a directed graph and it is divided into two cases, the consensus problem is discussed in these two cases. In addition, there exist some uncertain pinning connections between the followers and leader due to switching topologies, the distributed control protocol is designed to satisfy the follower asymptotically converge to the leader. By constructing suitable multiple Lyapunov functions and utilizing tools of M-matrix theory, some sufficient consensus criteria are deduced to reach this goal. Finally, two examples are given to verify the correctness of the proposed method.