bluuebunny/crossref_metadata_2025_split huggingface dataset:
Do circulating neutrophil extracellular traps predict recurrence in early breast cancer?
Authors: Martinez-Cannon Bertha Alejandra , Garcia-Ronquillo Karen , Rivera-Franco Monica M. , Leon-Rodriguez Eucario 
Published: January 2023

BackgroundNeutrophil extracellular traps (NETs), three-dimensional structures formed by neutrophil enzymes such as neutrophil elastase (NE) and nuclear components (DNA), have been associated with progression and metastasis in breast cancer (BC). Thus, the aim of this study was to evaluate the association of circulating NETs with clinicopathological characteristics and outcomes in early BC.MethodsA prospective cohort included women with newly diagnosed early BC. NETs were defined as the presence of NE-DNA complexes in plasma, measured by optical density. Levels of NETs were dichotomized according to the median, as low and high levels of circulating NETs. Fisher’s exact test was used to evaluate associations between NETs and clinicopathological characteristics and outcomes. Survival was assessed using the Kaplan Meier method and log-rank test.ResultsForty patients were included, 23 (57.5%) patients with low and 17 (42.5%) with high levels of circulating NETs. No associations were found between clinicopathological characteristics and circulating NETs levels. Recurrence (p = 0.99) and site of recurrence (p = 0.99) were not statistically associated with plasma NETs levels. Overall, recurrence-free survival was not statistically different between circulating levels of NETs.ConclusionsWith a short follow-up and low number of events, our results suggest that circulating levels of NETs at diagnosis of early BC are not associated with more aggressive clinicopathological characteristics, recurrence, or site of recurrence.
Presentation and management outcomes of Retinoblastoma among Syrian refugees in Jordan
Authors: Yousef Yacoub A. , Abu Salim Qusai F. , Mohammad Mona , Jaradat Imad , Mehyar Mustafa , AlJabari Reem , Al-Habahbeh Omar , Saboubeh Khalid , Halalsheh Hadeel , Khzouz Jakub , Shawagfeh Munir , Sultan Iyad , AlMasri Mahmoud , Al-Nawaiseh Ibrahim , Al-Hussaini Maysa , Mansour Asem 
Published: January 2023

PurposeThe humanitarian crisis in Syria has had a profound impact on the entire region. In this study, we report the patterns of presentation and management outcomes of Syrian patients with Retinoblastoma (Rb) treated at a single tertiary cancer center in Jordan.Methods and MaterialsThis is a retrospective comparative study of Syrian refugees and Jordanian citizens who had Rb between 2011 and 2020. Collected data included patient demographics, presentation, tumor stage, treatment modalities, eye salvage rate, metastasis, and mortality.ResultsThirty Syrian refugees (16 (53%) had bilateral disease) and 124 Jordanian citizens (51(41%) had bilateral disease) were diagnosed with Rb during this period. The median age at diagnosis for refugees was 10 and 32 months for patients with bilateral and unilateral Rb consecutively, compared to 6 and 28 months for citizens. The median lag time between signs of disease and initiation of treatment was 3 months for refugees, compared to 1 month for citizens.Refugees were more likely to present with a more advanced stage (p=0.046). Out of 46 affected eyes in refugees; 32 (70%) eyes were group D or E, while out of 175 affected eyes among citizens; 98 (56%) eyes were group D or E. Therefore, refugees with Rb were more likely to mandate primary enucleation (48%) compared to citizens (25%) (p=0.003). However, out of 24 eyes among refugees who received conservative therapy, 15 (62%) eyes were successfully salvaged, while out of 131 affected eyes among citizens who received conservative therapy, 105 (80%) eyes were successfully salvaged (p=0.06). Two (7%) of the refugees and four (3.2%) of the citizens with Rb died from metastasis.ConclusionSyrian refugees with Rb presented with more advanced disease due to delay in diagnosis and referral that increased the treatment burden by decreasing the chance for eye globe salvage. However, patients who received the timely intervention had a similar outcome to citizens with Rb; probably a reflection of the management of all patients at a single specialized center. We advocate for the timely referral of refugees with this rare life-threatening tumor to a specialized cancer center for the best possible outcome.
Identification of lymph node metastasis-related genes and patterns of immune infiltration in colon adenocarcinoma
Authors: Zhang Haoxiang , Zhao Guibin , Zhu Guangwei , Ye Jianxin 
Published: January 2023

BackgroundsColon adenocarcinoma(COAD) is one of the most common tumors of the digestive tract. Lymph node metastasis (LNM) is a well-established prognostic factor for COAD. The mechanism of COAD lymph node metastasis in immunology remains unknown. The identification of LNM-related biomarkers of COAD could help in its treatment. Thus, the current study was aimed to identify key genes and construct a prognostic signature.MethodsGene expression and clinical data were obtained from The Cancer Genome Atlas (TCGA) database. Differentially expressed genes were calculated by using R software. GO functional and KEGG pathway enrichment analysis were processed. The CIBERSORT algorithm was used to assess immune cell infiltration. STRING database was used to screen key genes and constructed a protein-protein interaction network (PPI network). The LASSO-Cox regression analysis was performed based on the components of the PPI network. The correlation analysis between LNM-related signature and immune infiltrating cells was then investigated. TISIDB was used to explore the correlation between the abundance of immunomodulators and the expression of the inquired gene.ResultsIn total, 394 differentially expressed genes were identified. After constructing and analyzing the PPI network, 180 genes were entered into the LASSO-Cox regression model, constructing a gene signature. Five genes(PMCH, LRP2, NAT1, NKAIN4, and CD1B) were identified as LNM-related genes of clinical value. Correlation analysis revealed that LRP2 and T follicular helper cells (R=0.34, P=0.0019) and NKAIN4 and T follicular helper cells (R=0.23, P=0.041) had significant correlations. Immunologic analysis revealed that LRP2 and NKAIN4 are potential coregulators of immune checkpoints in COAD.ConclusionIn general, this study revealed the key genes related to lymph node metastasis and prognostic signature. Several potential mechanisms and therapeutic and prognostic targets of lymph node metastasis were also demonstrated in COAD.
Prediction of necrotizing enterocolitis in very low birth weight infants by superior mesenteric artery ultrasound of postnatal day 1: A nested prospective study
Authors: Yue Guang , Wang Jun , Yang Sheng , Deng Ying , Wen Yang , Jia Wen , Cao Huiling , Ju Rong , Shi Yuan 
Published: January 2023

BackgroundNecrotizing enterocolitis (NEC) is a devastating intestinal complication that occurs mainly in very-low-birth-weight infants (VLBWI). The study's aim was to investigate the possibility of early prediction of NEC on postnatal day 1 based on superior mesenteric artery (SMA) doppler ultrasonograpy.MethodsA prospective, observational, nested case control study (ChiCTR1900026197) was conducted to enroll VLBWIs (birth weight &lt;1,500 grams) between October 2019 and September 2021. Doppler ultrasound measurement was done during the first 12 h of life and before first feeding. Infants developing NEC (stage II or III) subsequently were included in NEC group and infants spare of NEC were included in control group.Results370 VLBWIs were enrolled (30 NEC cases). Among the ultrasound parameters, S/D was significantly higher in the NEC group (OR: 2.081, 95% CI: 1.411–3.069, P = 0.000). The area under the receiver operating curve (AUROC) following the Logistic regression was 0.704 (95% CI: 0.566–0.842, P = 0.001). The sensitivity of S/D for predicting NEC was 52.2% and the specificity was 92.7%. The critical value of S/D was 6.944 and Youden index was 0.449. Preplanned subgroup analysis confirmed that NEC infants of different stages were characterized by different SMA bloodstream. Small for gestational age (SGA) might be a confounding factor affecting intestinal bloodflow. And infants with delayed initiation or slow advancement of feeding exhibited characteristic intestinal perfusion.ConclusionsIn VLBWI, early SMA ultrasound shows the potential to predict NEC. It is reasonable to speculate that SMA bloodstream is related to intestinal structural and functional integrity.
Genome-wide identification of the B3 gene family in soybean and the response to melatonin under cold stress
Authors: Ren Chunyuan , Wang Huamei , Zhou Zhiheng , Jia Jingrui , Zhang Qi , Liang Changzhi , Li Wanting , Zhang Yuxian , Yu Gaobo 
Published: January 2023

IntroductionMelatonin is a multipotent molecule that exists widely in animals and plants and plays an active regulatory role in abiotic stresses. The B3 superfamily is a ubiquitous transcription factor with a B3 functional domain in plants, which can respond temporally to abiotic stresses by activating defense compounds and plant hormones. Despite the fact that the B3 genes have been studied in a variety of plants, their role in soybean is still unknown.MethodsThe regulation of melatonin on cold resistance of soybean and the response of B3 genes to cold stress were investigated by measuring biochemical indexes of soybean. Meanwhile, the genome-wide identification of B3 gene family was conducted in soybean, and B3 genes were analyzed based on phylogeny, motifs, gene structure, collinearity, and cis-regulatory elements analysis.ResultsWe found that cold stress-induced oxidative stress in soybean by producing excessive reactive oxygen species. However, exogenous melatonin treatment could increase the content of endogenous melatonin and other hormones, including IAA and ABA, and enhance the antioxidative system, such as POD activity, CAT activity, and GSH/GSSG, to scavenge ROS. Furthermore, the present study first revealed that melatonin could alleviate the response of soybean to cold stress by inducing the expression of B3 genes. In addition, we first identified 145 B3 genes in soybean that were unevenly distributed on 20 chromosomes. The B3 gene family was divided into 4 subgroups based on the phylogeny tree constructed with protein sequence and a variety of plant hormones and stress response cis-elements were discovered in the promoter region of the B3 genes, indicating that the B3 genes were involved in several aspects of the soybean stress response. Transcriptome analysis and results of qRT-PCR revealed that most GmB3 genes could be induced by cold, the expression of which was also regulated by melatonin. We also found that B3 genes responded to cold stress in plants by interacting with other transcription factors.DiscussionWe found that melatonin regulates the response of soybean to cold stress by regulating the expression of the transcription factor B3 gene, and we identified 145 B3 genes in soybean. These findings further elucidate the potential role of the B3 gene family in soybean to resist low-temperature stress and provide valuable information for soybean functional genomics study.
The impact of time spent working from home on affective commitment in the workplace: The mediating role of social relationships and collective aims
Authors: Simon Adél Csenge , Aranyi Gabor , Faragó Klára , Pachner Orsolya Csilla , Kiss Orhidea Edith 
Published: January 2023

IntroductionWorking from home has become increasingly prevalent due to the COVID-19 pandemic, creating new challenges for organizations and employees. According to the latent deprivation model proposed by Jahoda, work provides latent benefits alongside its material rewards, and losing such benefits leads to a decline in well-being. Organizational affective commitment, or affective commitment within the organization, is a prominent concept in organizational psychology that is linked to lower workforce fluctuation and increased work performance. The present research examined the impact of time spent working from home on affective commitment by examining Jahoda’s “latent functions,” including social contact and collective purpose, representing an innovative application of the latent deprivation model in the context of home office.MethodsUsing an online questionnaire, we collected data from 456 participants (239 female and 217 male) who had been employed for at least 2 years and who had spent a proportion of their time working from home in March and April 2021. The data were analyzed using a path model, in which the potential adverse effect of time spent in home office on affective commitment to the workplace was mitigated by latent functions.ResultsSpecifically, we found that more time spent in home office was associated with a decrease in social contact, the impact of which on affective commitment was mediated through the perception of collective purpose.DiscussionOur findings emphasize the role of the latent benefits of work experienced by employees even when working from home, and the role of those benefits in supporting employees’ commitment to the workplace. We argue that a deeper understanding of such factors is vital, as working from home is expected to remain widespread even after the pandemic.
Psychological assessment of parents of people diagnosed with borderline personality disorder and comparison with parents of people without psychological disorders
Authors: Guillén Verónica , Bolo Sara , Fonseca-Baeza Sara , Pérez Sandra , García-Alandete Joaquín , Botella Cristina , Marco José Heliodoro 
Published: January 2023

BackgroundTo date, several evidence-based interventions have been created to help relatives of people with Borderline Personality Disorder (BPD), but few studies have analyzed the clinical situation of the family members. The aim of this study was twofold: (1) to explore the clinical symptomatology in a sample of parents of people diagnosed with BPD and compare them with a sample of a sample of people without a relative with a personality disorder, (2) to explore whether the parents of people diagnosed with BPD have psychopathology related to personality disorders (PD) or meet the diagnostic criteria for PD.MethodParticipants were 42 (39.6%) fathers and 64 (60.4%) were mothers and mothers (n = XX, −%) of people diagnosed with BPD, who were selected from a specialized PD unit for treatment. The sample of people without a relative with a PD was obtained from social network announcements. To test for differences between the two groups, Student’s t tests were performed for quantitative variables, and Chi-square tests were performed for categorical variables. Cohen’s d was calculated as a measure of the effect size.ResultsParents of people with BPD showed greater depressive and anxious symptomatology, higher levels of expressed emotion, and worse quality of life than the sample of people without a relative with a personality disorder. In addition, a high percentage of the parents of people diagnosed with BPD (50%) met the diagnostic criteria for different PD.ConclusionParents of people diagnosed with BPD may need psychological help in various aspects. Therapists are therefore advised to bear in mind the importance of carrying out a psychological assessment of family members and, if necessary, to offer psychological intervention. It is crucial to invite the family to be part of the treatment, since they can be part of the solution.Clinical Trial registration: ClinicalTrials.gov ID, NCT04160871 (registered November 15, 2019).
Posner-Schlossman syndrome relapse following inactivated COVID-19 vaccination in China
Authors: Sheng Qilian , Sun Yanan , Zhai Ruyi , Fan Xintong , Ying Yue , Kong Xiangmei 
Published: January 2023

IntroductionThis retrospective study aims to present the characteristics of Posner-Schlossman syndrome (PSS) relapse following inactivated COVID-19 vaccination.MethodsFrom 2020 to 2022, 12 out of 106 PSS patients undergoing relapses after any dose of inactivated COVID-19 vaccines were enrolled. Medical histories, information on the vaccination and systemic adverse events were collected. Patients were treated with corticosteroids, intraocular pressure (IOP)-lowering drugs and systemic immunosuppressive agents (if needed). Daily regimen and release course were noted.ResultsThe recurrence rate after vaccination was 11.32% (12/106, 95% CI: 5.29%–17.35%) among 106 PSS patients we surveyed. All the 12 patients were inoculated with inactivated COVID-19 vaccines developed by Sinopharm, China. The mean time of relapse was 5.27 ± 3.72 days (range: 1–13 days, median: 4 days). Higher IOP and more keratic precipitates (KPs) were seen in the relapse following vaccination (33.55 ± 12.99 mmHg, 91.67% had KPs compared to 25.38 ± 3.80 mmHg, 33.33% had KPs in previous relapse, P = 0.009). The mean release course was 30.71 ± 34.74 days for the relapse following vaccination and 7.33 ± 6.51 days for previous relapses. The attack frequency before and after vaccination was 3.56 ± 2.07 and 9.11 ± 7.34 times per year (P = 0.044). Higher daily doses of corticosteroids, IOP-lowering drugs and ganciclovir were needed to maintain stable course, though the difference did not reach statistical significance.DiscussionMore frequent relapses and harder control of IOP were found in PSS relapse following COVID-19 vaccination. Ophthalmologists need to be aware of the group vulnerability and take precautions, though the pathogenesis is still under investigation.
Causal effects and functional mechanisms of the Internet on residents' physical fitness-An empirical analysis based on China family panel survey
Authors: Zhang Long , Lu Chuntian , Yi Cuixia , Liu Zhipeng , Zeng Yuhua 
Published: January 2023

IntroductionPhysical fitness is an essential part of a healthy lifestyle that concerns the overall health of the nation. Research on the relationship between the Internet and physical fitness has long been caught in the dilemma of “media mobilization” and “media suppression,” and previous studies have rarely examined the causal relationship and functional mechanism.MethodsThis study selected the data of 23,989 samples successfully followed in all three surveys of the China Family Panel Survey (CFPS) from 2014 to 2018 to explore the correlation and causal inference between the Internet and physical fitness by using the Time Fixed Effects Model and cross-lagged models, respectively; meanwhile, the data of 24,687 samples in CFPS 2020 to examine the functional mechanism of the Internet's effect on residents' physical fitness behavior by the KHB method.ResultsWe obtained three valuable conclusions as follows: First, there is a significant correlation between the Internet and physical fitness behavior. Second, the Internet use is the cause for the increase in fitness frequency, and there is a rival relationship between Internet duration and fitness time. Third, under regular prevention and control of the COVID-19 epidemic, social capital and health risk perceptions are the functional mechanisms of the Internet influencing fitness behavior, and the mediating effect of psychological health risk perceptions is higher than that of social capital.DiscussionIt's necessary to create an intelligent, informative, and digital sports public service system by enriching and optimizing sports media and facilitating the Internet to serve residents' physical fitness better. The new concept of “Internet plus Fitness” will be of great significance in the implementation of the “Healthy China Initiative.”
ORIGO: A mission concept to challenge planetesimal formation theories
Authors: Marschall Raphael , Thomas Nicolas , Ulamec Stephan , Hviid Stubbe , Mottola Stefano , Vincent Jean-Baptiste , Ferri Francesca , Herique Alain , Plettemeier Dirk , Kereszturi Ákos , Lavagna Michèle R. , Prinetto Jacopo , Dottori Alice , Falke Albert , da Silva Pais Cabral Francisco 
Published: January 2023

Comets are generally considered among the most pristine objects in our Solar System. There have thus been significant efforts to understand these bodies. During the past decades, we have seen significant progress in our theoretical understanding of planetesimal/cometesimals (the precursors of comets) formation. Recent space missions—such as ESA’s Rosetta mission to comet 67P/Churyumov-Gerasimenko—have provided observations claimed by proponents of different comet formation theories to validate their scenarios. Yet, no single formation paradigm could be definitively proven. Given the importance of understanding how the first bodies in our Solar System formed, we propose a dedicated mission to address this issue. ORIGO will deliver a lander to the surface of a cometary nucleus where it will characterise the first five m of the subsurface. With remote sensing instruments and the deployment of payload into a borehole, we will be able to study the physico-chemical structure of ancient, unmodified material. The mission has been designed to fit into the ESA M-class mission budget.
Stingless bee keeping: Biocultural conservation and agroecological education
Authors: Aldasoro Maya Elda Miriam , Rodríguez Robles Ulises , Martínez Gutiérrez María Luisa , Chan Mutul Guelmy A. , Avilez López Teresita , Morales Helda , Ferguson Bruce G. , Mérida Rivas Jorge A. 
Published: January 2023

IntroductionStingless bee breeding, also called Meliponiculture, has existed for thousands of years in Mesoamerica among a variety of rural and indigenous cultures. Due to its biocultural importance, it represents a perfect device for agroecological education and scaling, as well as pollinator conservation. At the same time, promoting agroecological transitions are necessary for conserving the cultural heritage that meliponicultures represent.MethodsWe organized a series of activities based on this premise: documenting and dialoguing “saberes contemporáneos” (contemporary knowledges), design and implementation of community agroecological workshops, guided visits at the institutional Meliponary at ECOSUR Villahermosa and promotion of agroecological and biocultural school gardens, all in Tabasco, México. We used “diálogo de saberes” (knowledge dialogues) as a methodological approach, promoting respect for the contribution of the diversity of ontoepistemologies involved. We drew on the ideas of several pedagogues, mainly from popular education and critical pedagogy frameworks.Results and discussionWe identified characteristics of stingless bees and meliponicultures that make them excellent mediators for biocultural conservation and agroecological education: the complex, deep and beautiful relation between humans and bees; meliponiculture's ecological and cultural importance; stingless bees as pollinators par excellence, landscape connectors, and charismatic species; the association of bees with values around work and community; meliponicultures' symbolic relevance and emotional significance; meliponicultures as promoters of intergenerational dialogue; bee keeping as an activity of caring for the continuity of life; sensory stimulation through contact with colonies; learning through doing in the practice of beekeeping; meliponicultures as an activity requiring skill but no special equipment; and meliponiculture's productive potential. This confluence of teaching-learning opportunities, cultural and moral values, care for the land and biocultural diversity, and economic potential makes meliponiculture a potent catalyst for agroecological learning and transitions.