bluuebunny/crossref_metadata_2025_split huggingface dataset:
PHACES‐like syndrome with TMEM260 compound heterozygous variants
Authors: Kuroda Yukiko , Saito Yoko , Enomoto Yumi , Naruto Takuya , Mitsui Jun , Kurosawa Kenji 
Published: May 2023

AbstractPHACES syndrome is a multiple congenital disorder with unknown etiology that is characterized by Posterior fossa anomalies, Hemangioma, Arterial lesions, Cardiac abnormalities/coarctation of the aorta, Eye anomalies, and Sternal cleft. Compound heterozygous or homozygous TMEM260 variants cause structural heart defects and renal anomalies syndrome (SHDRA). We describe a 10‐year‐old male patient with a PHACES‐like syndrome and TMEM260 compound heterozygous variants who demonstrated overlapping phenotypes between the two syndromes. He presented with truncus arteriosus, supraumbilical raphe, ophthalmological abnormality, vertebral abnormality, borderline intellectual disability, and hearing loss. He had normal serum creatinine. In proband exome sequencing, compound heterozygous TMEM260 variants (NM_017799.4 c.1617delG p.(Trp539Cysfs*9)/c.1858C > T p.(Gln620*)) were identified. Twelve patients have been reported with TMEM260‐related SHDRA: 10 had truncus arteriosus and 6 had renal failure. One previously reported patient had facial port wine nevus and another patient had supraumbilical raphe, which are the cardinal signs for PHACES syndrome. TMEM260‐related SHDRA could share overlapping clinical features with PHACES syndrome. This report expands the phenotypic spectrum of a TMEM260‐related disorder.
Connecting Ruthenium Photocatalysis to 1,2‐Dioxetane‐Mediated Chemiluminescence: a Versatile Combination for Optical Detection and Read‐Out
Authors: Köckenberger Johannes , Thurston Ryan , Sauer Caroline , Oppl Janina , Heinrich Markus R. 
Published: May 2023

AbstractOptical storage and photon quantification systems based on sensitive photoreactions have numerous applications. Herein, we report a highly efficient photocatalytic reaction, in which ruthenium photoredox catalysis is combined with a 1,2‐dioxetane from which chemiluminescence can be triggered. In this system, blue light irradiation as optical input enables a defined inverse correlation with base‐triggered, blue light emission as optical output. Comparison of readout by 1H NMR and chemiluminescence, relative to previous optical input, underlines the reliability and usefulness of the ruthenium‐dioxetane system for optical storage, sensing and ruthenium detection.
Regulating Zinc Deposition via Zincophilic 2D‐Cu2Te as the Current Collector to Suppress Dendrite Formation toward High Performance Aqueous Zinc‐Ion Batteries
Authors: Tsai Ming‐Hsuan , Lin Tuan‐Yue , Su Tian‐Shun , Chen Guan‐Min , Liu Yu‐chen , Chen Yu‐Ze 
Published: May 2023

AbstractIn this study, we synthesized standing 2D δ‐Cu2Te flakes (δ‐CTFs) via a facile post‐tellurization process, which served as the current collector to accommodate zinc (Zn) for AZIBs. These flakes exhibited low nucleation overpotential and low interfacial impedance, facilitating the plating/stripping of Zn ions. Interestingly, the hydrophilicity and standing structure of δ‐CTFs guided the electrodeposited Zn to laterally grow on the surface of δ‐CTFs, effectively suppressing Zn dendrite formation. The Zn@δ‐CTFs anode exhibited a long‐term cycling duration of 510 hours in a symmetric cell, which is far superior to previous reports. Even under high current density of 10 mA cm−2, the anode was able to perform stably with a cycle life of 110 hours. The machine learning model was exploited to predict the effective charge value, discovering that Zn migrated in Cu2Te were subject to the larger driving force of migration under applied field. Finally, the Zn@δ‐CTFs//MnO2 full battery exhibited excellent rate‐dependent capacity and maintained a capacity of 100 mAh g−1 after 1000 cycles at a current density of 1 A g−1, without Zn dendrite formation. This research provides a new strategy for regulating Zn deposition to address dendrite issues toward long lifespan AZIBs.
Carbon‐based organic radical spin filters
Authors: Cho Daeheum 
Published: May 2023

AbstractSingle‐molecule magnetic junctions serve as an important component for the manipulation of spin‐polarized currents by chemical modifications and control of magnetic interactions in spintronic devices. We employed the nonequilibrium green function and spin‐polarized density functional theory simulations to investigate the performance of molecular junctions bearing an organic radical as spin filters. A spin‐polarized current could be generated using the junctions and, moreover, the spin‐filtering efficiency could be tuned by varying the number of magnetic units. Spin orbital and density of states analyses revealed the relationship effect of the shapes and energy levels of the dominant electron‐conducting channels in the spin‐filtering efficiency. Thus, the use of a tunable organic magnetic molecular junction system is an appealing approach for regulating spin‐polarized currents in spintronic devices.
Personalized circulating tumor DNA detection to monitor immunotherapy efficacy and predict outcome in locally advanced or metastatic non‐small cell lung cancer
Authors: Cheng Lei , Gao Guanghui , Zhao Chao , Wang Haowei , Yao Chao , Yu Hanchuanzhi , Yao Jichen , Li Feng , Guo Lijie , Jian Qijie , Chen Xiaoxia , Li Xuefei , Zhou Caicun 
Published: May 2023

AbstractObjectiveImmune checkpoint inhibitors (ICIs) or combined with chemotherapy exhibit substantial efficacy for the treatment of advanced non‐small cell lung cancer (NSCLC). However, reliable biomarkers that can monitor response to first‐line ICIs ± chemotherapy remain unclear.MethodsA total of 16 tumor tissues and 46 matched peripheral blood samples at baseline and during treatment were retrospectively collected from 19 locally advanced or metastatic NSCLC patients. The circulating tumor DNA (ctDNA) burden by tumor‐informed assay was detected to monitor and predict the therapeutic response and survival of NSCLC patients treated with first‐line ICIs or plus chemotherapy.ResultsWe found that ctDNA was only positively detected in one patient by tumor‐agnostic assay with a mean variant allele fraction (VAF) of 6.40%, whereas it was positively detected in three patients by tumor‐informed assay with a mean VAF of 8.83%, 0.154%, and 0.176%, respectively. Tumor‐informed assays could sensitively detect ctDNA in 93.75% (15/16) of patients. Trends in the level of ctDNA from baseline to first evaluation was consistent with the radiographic changes. There was a greater decrease in ctDNA after treatment compared with baseline in patients with partial response compared to patients with stable disease/progressive disease. Patients with over a 50% reduction in ctDNA had a significant progression‐free survival and overall survival benefit.ConclusionThe tumor‐informed assay was favorable for ctDNA detection, and early dynamic changes in plasma ctDNA may be a valuable biomarker for monitoring the efficacy and predicting the outcome in advanced NSCLC patients treated with first‐line ICIs ± chemotherapy.
Phytochemical and Biological Profile of Essential Oils of Elionurus muticus (Spreng.) Growing in Northeastern Argentina
Authors: Celaya Liliana S. , Le Vraux María A. , Heit Cecilia I. , Viturro Carmen I. , Martina Pablo F. 
Published: May 2023

AbstractThe purpose of this study was to investigate essential oils (EOs) from leaves of Elionurus muticus growing in Northeastern Argentina regarding their physicochemical profiles as well as their biological potential. Roots of a selected E. muticus population were investigated too. For this purpose, EOs of fresh materials were obtained by steam distillation and the chemical composition was characterized by gas chromatography GC/MS‐FID. Antibacterial, antioxidant and eco‐toxicity activities of the essential oils (EOs) were tested by in vitro assays. The EOs showed three E. muticus chemotypes: citral (neral+geranial), acorenone+bisabolone, acorenone+geranial. EO of roots of citral population contains mainly acorenone derivatives. EOs have high antibacterial effect against Staphylococcus aureus, being found minor antibacterial effect against Gram‐negative bacteria. The half‐maximal inhibitory concentration of EOs against DPPH⋅ were 7.1–30.0 mg/mL and the eco‐toxicity was high with LD50 <39 μg/mL. Based on the findings, given the high variability in their chemical composition and biological activity of E. muticus EO and the promising yields, it could be potentially chosen for industrial applications.
Synthesis and Conformational Analysis of Fullerene Torsion Balances for Assessing the Contribution of Dispersion and Anionic Character in Non‐Covalent Arene–Fullerene Interactions
Authors: Yamada Michio , Sahara Koki , Koizumi Masaaki , Maeda Yutaka , Suzuki Mitsuaki 
Published: May 2023

AbstractWe employ a molecular torsion balance displaying bifurcated conformational isomerism to quantitatively evaluate the non‐covalent interactions between the fullerene surface and substituted arene moieties containing elements with high atomic numbers, as well as the thermodynamic processes involved in the folding equilibrium using nuclear magnetic resonance spectroscopy. The interaction between fullerene and haloaryl groups was stronger in cases where the introduced halogen had a higher atomic number, indicating that dispersion forces play a significant role in the interaction between fullerenes and 4‐haloaryl groups. The dispersion term also significantly contributed to the interaction between fullerene and the 4‐mercaptophenyl group. Moreover, the addition of an appropriate base to the 4‐mercaptophenyl‐appended torsion balance formed the corresponding thiophenolate anion, resulting in a large negative change in the folding free energy in CDCl3. Detailed analysis suggested that the observed attractive anionic arene–fullerene interactions predominantly originated from solvation effects.
Nonorthogonal Cascade Catalysis in Multicompartment Micelles
Authors: Ahmed Eman , Cho Jinwon , Jang Seung Soon , Weck Marcus 
Published: May 2023

AbstractMulticompartment micelles (MCMs) containing acid and base sites in discrete domains are prepared from poly(norbornene)‐based amphiphilic bottlebrush copolymers in aqueous media. The acid and base sites are localized in different compartments of the micelle, enabling the nonorthogonal reaction sequence: deacetalization – Knoevenagel condensation – Michael addition of acetals to 2‐amino chromene derivatives. Computational simulations using dissipative particle dynamics (DPD) elucidated the bottlebrush composition required to effectively site‐isolate the nonorthogonal catalysts. This contribution presents MCMs as a new class of nanostructures for one‐pot multistep nonorthogonal cascade catalysis, laying the groundwork for the isolation of three or more incompatible catalysts to synthesize value‐added compounds in a single reaction vessel, in water.
The case for adjuvant BRAF‐targeted therapy versus adjuvant anti–PD‐1 therapy for patients with resected, high‐risk melanoma
Authors: Mooradian Meghan J. , Sullivan Ryan J. 
Published: May 2023

AbstractThe development of highly effective BRAF‐targeted therapy and immune checkpoint inhibition for patients with advanced metastatic melanoma has transformed the treatment of this disease. More recently, these advances have moved into the resected, high‐risk stage II and III settings. For patients with resected, BRAF‐mutant stage III melanoma, there are no head‐to‐head data to support the use of BRAF‐targeted therapy (specifically the combination of dabrafenib and trametinib) with either single‐agent nivolumab or pembrolizumab. Because the relapse‐free and distant metastasis–free survivals are similar in a cross‐trial comparison, it is not clear what the best option for these patients is. In this piece, the authors argue on behalf of and against both approaches.Plain Language Summary

Two types of therapy exist for patients diagnosed with melanoma who have completed surgery and remain at high risk for tumor recurrence: (1) drugs that target the BRAF mutation (found in ∼50% of patients) and (2) immunotherapy.

There are no data showing that either approach is better than the other, so the choice of which therapy is best for an individual patient can be challenging.

In this article, we make arguments for and against each option.
The Steady‐State ALTADENA RASER Generates Continuous NMR Signals**
Authors: Yang Jing , Wang Peng , Korvink Jan G. , J. Brandner Jürgen , Lehmkuhl Sören 
Published: May 2023

AbstractA RASER (Radio Amplification by Stimulated Emission of Radiation) facilitates the study of nonlinear phenomena, as well as the determination of NMR parameters with high precision. To achieve maximum sensitivity in the desired operating mode, it is crucial to control the RASER over long periods of time. So far, this was only possible at ultra‐low magnetic fields. Here, we introduce a way to control the operating regime of a RASER at a magnetic field of 1.45 T. We employ a continuous‐flow RASER, pumped by PHIP (ParaHydrogen Induced Polarization). The hydrogenation of vinyl acetate (VA) with parahydrogen provides the required negative polarization on the methyl group of the product ethyl acetate (EA). The protons within the methyl group, separated by a 7 Hz J‐coupling, are RASER active. This system demonstrates five RASER phenomena: inequivalent and equivalent amplitudes in the “normal NMR mode”, period doublings, frequency combs, and chaos. The experiments match with simulations based on a theoretical model of two nonlinear‐coupled RASER modes. We predict the RASER regime at set conditions and visualize the prediction in a bifurcation diagram.
Contract farming as an alternative to large‐scale land acquisition and promoting inclusive and responsible agricultural investment: Evidences from Ethiopia
Authors: Adam Achamyeleh Gashu , Agegnehu Alelegn Wenedem 
Published: May 2023

AbstractThe acquisition of large tracts of land for agricultural investment from developing countries, including Ethiopia, by commercial firms has been aggravated after global food crisis in 2007–2008. Given the current attention to large‐scale land acquisition or land grabbing phenomenon, contract farming has been identified as a participatory and inclusive farming model which does not require agricultural firms to purchase or lease land in order to operate agricultural investments. Contract farming enables local smallholder farmers to produce agricultural products on their own land and ensure the benefits of land‐based agricultural investments remain with them. Unfortunately, the understanding and practice of contract farming as an alternative to large‐scale land acquisition to protect the land rights of the local people is underexplored in Ethiopia. This study, therefore, is aimed to examine the practice and conceptual understanding of contract farming in the context of agricultural investment in Ethiopia. To achieve this objective, mixed research approach was employed. Intensive document reviews, questionnaire, key informant interview and FGD were used as data collection tools. The results show that the practice of contract farming in Ethiopia is very insignificant and commercial farming is almost entirely relied on land acquisition. Moreover, gaps in policy and legal framework to guide contract farming has made difficult to effectively implement contract framing at national level. Finally, this study advised to introduce contract framing as a viable alternative to land acquisition for agricultural investment that fosters local smallholder farmers' participation in agricultural production and contributes to sustainable development.
OMIP‐91: A 27‐color flow cytometry panel to evaluate the phenotype and function of human conventional and unconventional T‐cells
Authors: Anderson Jeremy , Jalali Sedi , Licciardi Paul V. , Pellicci Daniel G. 
Published: May 2023

AbstractThis 27‐color panel was developed to simultaneously measure different T‐cell populations (CD4, CD8, γδ T‐cells, and MAIT cells) and their subsets (Memory, Th1, Th2, Th17, Tfh, and Treg) along with functional markers associated with their activation status, cytokine production and cytotoxicity. This panel will be useful for both in vivo and in vitro studies evaluating T‐cells in the context of human health and disease. This panel is valuable in settings where samples are limited as a large amount of data will be generated using small volumes of blood.
Transcriptomic analysis reveals the role of SIX1 in mouse cranial neural crest patterning and bone development
Authors: Baxi Aparna , Jourdeuil Karyn , Cox Timothy C. , Clouthier David E. , Tavares Andre L. P. 
Published: May 2023

AbstractBackgroundGenetic variants of the transcription factor SIX1 and its co‐factor EYA1 underlie 50% of Branchio‐oto‐renal syndrome (BOR) cases. BOR is characterized by craniofacial defects, including malformed middle ear ossicles leading to conductive hearing loss. In this work, we expand our knowledge of the Six1 gene regulatory network by using a Six1‐null mouse line to assess gene expression profiles of E10.5 mandibular arches, which give rise to the neural crest (NC)‐derived middle ear ossicles and lower jaw, via bulk RNA sequencing.ResultsOur transcriptomic analysis led to the identification of 808 differentially expressed genes that are related to translation, NC cell differentiation, osteogenesis, and chondrogenesis including components of the WNT signaling pathway. As WNT signaling is a known contributor to bone development, we demonstrated that SIX1 is required for expression of the WNT antagonist Frzb in the mandibular arch, and determined that SIX1 expression results in repression of WNT signaling.ConclusionOur results clarify the mechanisms by which SIX1 regulates the development of NC‐derived craniofacial elements that are altered in SIX1‐associated disorders. In addition, this work identifies novel genes that could be causative to this birth defect and establishes a link between SIX1 and WNT signaling during patterning of NC cells.
Peatlands promote fire refugia in boreal forests of northern Alberta, Canada
Authors: Kuntzemann Christine E. , Whitman Ellen , Stralberg Diana , Parisien Marc‐André , Thompson Dan K. , Nielsen Scott E. 
Published: May 2023

AbstractIn the boreal forests of North America, large wildfires often leave residual patches of unburned vegetation, termed fire refugia, which can affect post‐fire ecosystem processes. Although topographic complexity is a major driver of fire refugia in mountainous terrain, refugia in boreal plains are more likely driven by a combination of other bottom‐up controls on fuel configuration as well as top‐down climate controls. In this study, we investigated the role of hydrological, ecological, and topographic heterogeneity, as well as climate moisture patterns, on the presence of fire refugia in forested upland and peatland ecosystems within Alberta's subhumid boreal forests over a 33‐year (1985–2018) period. Generalized linear models were used to model the probability of refugia in forested stands as a function of bottom‐up (vegetation, topography, site moisture, and ecosystem) and top‐down (normal and annual climate moisture deficit) controls. We then developed predictive maps of refugia probability for a range of normal and interannual climate moisture deficit values. We found that forested fens had a probability of refugia that was 64% higher than upland forests, while forested bogs did not differ from forested uplands in refugia likelihood. Climate and physical setting presented the strongest controls on fire refugia in uplands and peatlands, respectively. Increasing amounts of adjacent bogs, but not fens, produced a sixfold increase in refugia probability in uplands, while increasing amounts of adjacent bogs and fens produced roughly two times the refugia probability in forested peatlands. In these upland forest stands, fire refugia probability was negatively related to the interaction between regional climate moisture deficits and interannual deviations from these norms, thus increasing the probability of fire refugia during more severe drought conditions in areas with less arid climates, while decreasing refugia probabilities in drier climates. However, in peatlands themselves, neither regional climate moisture conditions nor the interannual deviations affected refugia. Fire size had a negative effect on fire refugia in all upland‐based models and a positive effect in all peatland‐based models. Our results suggest that large areas of intact peatlands may be capable of promoting fire refugia and thereby slowing climate‐driven, fire‐mediated vegetation transitions in surrounding forest ecosystems.
Water‐Soluble Fe(II) Complexes for Theranostic Application: Synthesis, Photoacoustic Imaging, and Photothermal Conversion
Authors: Delcroix Maeva , Reddy Marri Anil , Parant Stéphane , Gros Philippe C. , Bouché Mathilde 
Published: May 2023

AbstractSignificant effort focused on developing photoactivatable theranostics for localized image guided therapy of cancer by thermal ablation. In this context iron complexes were recently identified as photoactivatable theranostic agents with adequate biocompatibility and body clearance. Herein, a series of FeII complexes bearing polypyridine or N‐heterocyclic carbenes is reported that rely on rational complex engineering to red‐shift their MLCT based excited‐state deactivation via a straightforward approach. The non‐radiative decay of their MLCT upon irradiation is exploited for theranostic purposes by combining both tracking in photoacoustic imaging (PA) and photothermal therapy (PTT). The influence of structural modifications introduced herein on the solubility and stability of the complexes in biorelevant aqueous media is discussed. The relationship between complexes’ design, production of contrast in photoacoustic and photothermal efficiency are explored to develop tailored PA/PTT theranostic agents.
Functional Dithienopyrazines: Structure‐Property Relationships**
Authors: Kreuzer Franziska , Mena‐Osteritz Elena , Bäuerle Peter 
Published: May 2023

AbstractDithienopyrazines are only scarcely used as building blocks in organic electronic materials. Here, we report efficient preparation and investigation of syn‐ and anti‐dithienopyrazines, which were functionalized with triaraylamine units to provide different series of donor‐acceptor‐donor‐type materials. The characterization of the optoelectronic properties resulted in valuable structure‐property relationships and allowed for the elucidation of the influence of structural effects such as core structure (syn vs anti), type of substituents (directly arylated vs ethynylated aryl), and substitution pattern (α,α’‐ vs β,β’‐ vs fourfold substitution). Finally, first application of a dithienopyrazine derivative as model for hole‐transport materials tailored for organic electronic devices has been realized.