bluuebunny/crossref_metadata_2025_split huggingface dataset:
Inhibition of CD73 expression or A2AR blockade reduces MRP1 expression and increases the sensitivity of cervical cancer cells to cisplatin
Authors: Carrera‐Martínez Monserrat , Mora‐García María de L. , García‐Rocha Rosario , Weiss‐Steider Benny , Montesinos‐Montesinos Juan J. , Hernández‐Montes Jorge , Don‐López Christian A. , Monroy‐García Alberto 
Published: February 2023

AbstractRecently, a link between the biological activity of CD73 in solid tumors and multidrug resistance protein (MRP) has been proposed. Cisplatin (CP) is the most widely used anticancer agent to treat advanced and recurrent cervical cancer (CC). However, multidrug resistance protein‐1 (MRP1) is overexpressed in approximately 85% of these tumors and has been strongly associated with cisplatin resistance (CPR). In this study, we examine the involvement of CD73 and the interaction of adenosine (ADO) with its receptors (ARs) in MRP1 expression in CC cells. We found that ADO positively modulates MRP1 expression in CC cells in a dose‐dependent manner. The inhibition of CD73 expression with a CD73‐targeted siRNA and A2AR blockade with the selective antagonist ZM241385 significantly decreased MRP1 expression and the extrusive capacity of CC cells, making them significantly more sensitive to CP treatment than cancer cells treated with MK‐751, a specific MRP1 inhibitor. These results suggest CD73 inhibition or blocking ADO signaling through A2AR could be strategies to reverse CPR in patients with advanced or recurrent CC, which is characterized by very low response rates to CP (10%–20%).
A case of hemorrhagic shock in a patient with neurofibromatosis type 1
Authors: Sakaguchi Yutaro , Okano Hiromu , Furuya Ryosuke , Otsuka Tsuyoshi , Miyazaki Hiroshi 
Published: February 2023

AbstractComplications of neurofibromatosis type 1 include fatal bleeding events due to vascular fragility. In this case of hemorrhagic shock due to a neurofibroma, the bleeding was controlled using an occlusion balloon and endovascular treatment which stabilized the patient. Systemic vascular investigation for bleeding sites is important to prevent fatal outcomes.
Structure Elucidation of Lithium Compounds Using 7Li Residual Quadrupolar Couplings
Authors: Rüttger Franziska , Patten Tim , Kretsch Johannes , Krawczuk Anna , Stalke Dietmar , John Michael 
Published: February 2023

AbstractA new NMR method for the structure elucidation of lithium compounds under solution‐like conditions is presented. It is based on the measurement of 7Li residual quadrupolar couplings (RQCs) in a stretched polystyrene (PS) gel, and comparison to RQCs predicted from crystal or DFT‐derived model structures in combination with alignment tensors derived from one‐bond 1H,13C residual dipolar couplings (RDCs). The method was applied to five lithium model complexes containing monoanionic, bidentate bis(benzoxazole‐2‐yl)methanide, bis(benzothiazole‐2‐yl)methanide and bis(pyridyl)methanide ligands, of which two are first introduced in this work. In agreement with the crystalline state, four complexes are monomeric with Li coordinated fourfold by two additional THF molecules, whereas in one complex bulky tBu groups only provide space for one additional THF molecule.
Reactivity of Silylene with Gallium‐ and Boron Trihalide at Reductive Conditions Resulting in Unforeseen Products
Authors: Nazish Mohd , Harinath Adimulam , Lüert Daniel , Köhler Christian , Herbst‐Irmer Regine , Stalke Dietmar , Roesky Herbert W. 
Published: February 2023

AbstractHerein, we report a one‐pot reaction of gallium and boron halides with potassium graphite in the presence of benzamidinate stabilized silylene LSi‐R, (L=PhC(NtBu)2). The reaction of LSiCl with an equivalent amount of GaI3 in the presence of KC8 leads to the direct substitution of one chloride group by gallium diiodide simultaneously additional coordination of silylene resulted in L(Cl)Si→GaI2‐Si(L)→GaI3 (1). In compound 1, the structure comprises two differently coordinated gallium atoms where one gallium presents between two silylenes and the other gallium is only coordinated by one silylene. In this Lewis acid‐base reaction the oxidation states of the starting materials remain unchanged. The same is valid in the silylene boron adduct formation of L(tBu)Si‐BPhCl2 (2) and L(tBu)Si‐BBr3 (3). This new route provides access to galliumhalosilanes challenging to synthesize by any other method.
Antibodies Directed Against GalNAc‐ and GlcNAc‐O‐Tyrosine Posttranslational Modifications – a New Tool for Glycoproteomic Detection
Authors: Behren Sandra , Schorlemer Manuel , Schmidt Gudula , Aktories Klaus , Westerlind Ulrika 
Published: February 2023

AbstractIn the last decade, it was discovered that protein mucin‐type O‐glycosylation and O‐GlcNAcylation modify Tyr residues besides the well explored Thr and Ser amino acids. Several glycoproteomic studies have identified α‐GalNAc‐O‐Tyr modifications, and studies propose that β‐GlcNAc‐O‐Tyr also exists as a new group of posttranslational modifications (PTMs). Specific bacterial toxins have further been identified to modify host GTPases with α‐GlcNAc‐O‐Tyr to promote bacterial virulence. Despite being identified on numerous proteins, the biological roles, biosynthesis and expression of GalNAc‐ and GlcNAc‐O‐Tyr modifications are poorly understood. A major obstacle is the lack of tools to specifically detect and identify proteins containing these modifications. With this in mind, we prepared vaccine constructs and raised antibodies to enable selective detection of proteins carrying these new PTMs. The obtained polyclonal antibody sera were evaluated using ELISA and glycopeptide microarrays and were found to be highly selective for GlcNAc‐ and GalNAc‐O‐Tyr glycopeptides over the corresponding Ser‐ and Thr‐modifications. For microarray analysis, synthetic GlcNAc‐ and GalNAc‐O‐Tyr Fmoc‐amino acids were prepared and applied in Fmoc‐SPPS to obtain an extensive O‐glycopeptide library. After affinity purification, the antibodies were applied in western blot analysis and showed specific detection of α‐GlcNAc‐O‐Tyr modified RhoA GTPase.
Light‐Triggered Reversible Swelling of Polymeric Spheres via Surfactant‐Free RAFT Emulsion Polymerization‐Induced Self‐Assembly**
Authors: Deng Zichao , Zhang Chengyun , Sun Yalan , Wang Rui , Chen Tianhao , Liu Zhefeng , Chen Aihua 
Published: February 2023

AbstractResponsive photonic crystals (RPCs) assembled by monodisperse colloidal particles have attracted enormous interest recently due to their tremendous applications in smart devices. Their structural colors can be determined by particle sizes. However, the lack of a reliable way to tune the sizes in situ limits their development. Herein, we present an efficient route to solve this problem through the fabrication of spherical polymeric particles with light‐triggered reversible swelling behavior via surfactant‐free reversible addition‐fragmentation chain transfer (RAFT) emulsion polymerization‐induced self‐assembly (PISA). Amphiphilic macro‐RAFT agents containing azobenzene groups were synthesized and subsequently employed to mediate the polymerization of methyl methacrylate. Uniform submicron spheres were obtained by modulating solid contents and other parameters. Benefiting from the photoisomerization of azobenzene moieties, the particle sizes expanded and contracted upon alternative ultraviolet/visible‐light irradiation accordingly. This strategy will be a supplement to the emulsion PISA and especially give aid to the progress of the RPC materials.
Recent Advances in Growth, Properties and Applications of 2D Inorganic Molecular Crystals†
Authors: Li Menghan , Guo Yang , Li Lin , Geng Dechao , Hu Wenping 
Published: February 2023

Comprehensive SummarySince the concept of 2D inorganic molecular crystals (2DIMCs) was introduced, intensive attentions have been gradually devoted to this field. Herein, the very recent advances in growth, properties, and applications of 2D inorganic molecular crystals are comprehensively reviewed. Firstly, the newly emerged 2DIMCs are classified into three categories. Then the two typical production methods are present, thus leading to discrepancy in size, distribution, morphology and structures of 2DIMCs. Moreover, the unique physicochemical properties of 2DIMCs are demonstrated on the basis of the as‐obtained 2DIMCs, the various applications are exhibited, demonstrating significant potential in related fields. Finally, the perspective and prospects are outlooked to offer insights in further development of 2DIMCs.
Discovery of Antitrypanosomal Indolylacetamides by a Deconstruction–Optimization Strategy Applied to Paullones
Authors: Lindhof Jens C. , Ihnatenko Irina , Müller Marco J. , Orban Oliver C. F. , Ortíz Cecilia , Benítez Diego , Dibello Estefanía , Seidl Leonardo L. , Comini Marcelo A. , Kunick Conrad 
Published: February 2023

AbstractThe parasitic kinetoplastid diseases Leishmaniasis, Chagas disease and Human African Trypanosomiasis constitute serious threats for populations throughout the (sub‐)tropics. Most available drugs to treat these diseases possess inadequate properties and candidates to fill the drug pipeline are urgently needed. Paullone‐N5‐acetamides inhibit trypanothione synthetase (TryS), an essential kinetoplastid enzyme, and exhibit antiparasitic activity in the low micromolar range, but lack the desired selectivity against mammalian cells (selectivity index (SI):<10). With the aim to identify the paullones’ moieties responsible for TryS inhibition and bioactivity, we applied molecular simplification and ring disconnection approaches. The new indolylacetamides lost activity against the expected molecular target (TryS) compared to the reference paullone MOL2008 (Leishmania infantum TryS IC50 : 150 nM; Trypanosoma brucei bloodstream form EC50: 4.3 μM and SI: 2.4). However, several of them retained potency (T. b. brucei EC50: 2.4–12.0 μM) and improved selectivity (SI: 5 to >25).
Identification of quantitative trait loci for fiber quality, yield, and plant height traits in Upland cotton
Authors: Shrestha Navin , Zhang Kuang , Gowda S. Anjan , Abdelraheem Abdelraheem , Jones Don C , Kuraparthy Vasu 
Published: February 2023

AbstractIn cotton, most agronomic traits are controlled by polygenes. In this study, 110 F6 recombinant inbred lines (RILs), derived from Upland cotton cross NC05AZ06 x NC11‐2100, were used to develop a linkage map and to identify quantitative trait loci (QTL) for six fiber quality traits, three yield traits, and plant height. These RILs were genotyped using the CottonSNP63K array and phenotyped for fiber quality, yield traits, and plant height in 2‐year field trials. Analysis of variance revealed significant (p < 0.05) differences among RILs for all traits studied, and the heritability estimates were moderate (30%–60%) to high (> 60%). Both positive and negative correlations were observed for fiber quality and yield traits. A total of 3,774 polymorphic SNP markers were used to develop a genetic map with an average marker density of 1.54 SNP/cM. Thirty QTL for fiber quality traits, yield traits, and plant height were detected on 15 different chromosomes, explaining 6.80%–20.02% of the phenotypic variance (PVE). Of these, 14 were major QTL (PVE > 10%), and three major QTL were detected in both years. Candidate gene analysis in the major QTL detected in both years and plant height QTL with PVE of 20.02% revealed five putative genes for fiber quality traits and one putative gene for plant height. The linkage map and identified QTL along with the candidate genes in the study could serve as additional breeding resources for Upland cotton genetic improvement.
Artificial Photoenzymatic Reduction of Carbon Dioxide to Methanol by Using Electron Mediator and Co‐factorAssembled ZnIn2S4 Nanoflowers
Authors: Zhao Hongqing , Qi Yanou , Zhan Peng , Zhu Qian , Liu Xiangshi , Guan Xinyao , Zhang Chenxi , Su Changsheng , Qin Peiyong , Cai Di 
Published: February 2023

AbstractIncreased absorption of visible light, low electron‐hole recombination, and fast electron transfer are the major objectives for highly effective photocatalysts in biocatalytic artificial photosynthetic systems. In this study, a polydopamine (PDA) layer containing electron mediator, [M], and NAD+ cofactor was assembled on the outer surface of ZnIn2S4 nanoflower, and the as‐prepared nanoparticle, ZnIn2S4/PDA@poly/[M]/NAD+, was used for photoenzymatic methanol production from CO2. Because of effective capturing of visible light, reduced distance of electron transfer, and elimination of electron‐holes recombination, a high NADH regeneration of 80.7±1.43 % could be obtained using the novel ZnIn2S4/PDA@poly/[M]/NAD+. In the artificial photosynthesis system, a maximum methanol production of 116.7±11.8 μm was obtained. The enzymes and nanoparticles in the hybrid bio‐photocatalysis system could be easily recovered using the ultrafiltration membrane at the bottom of the photoreactor. This is due to the successful immobilization of the small blocks including the electron mediator and cofactor on the surface of the photocatalyst. The ZnIn2S4/PDA@poly/[M]/NAD+ photocatalyst exhibited good stability and recyclability for methanol production. The novel concept presented in this study shows great promise for other sustainable chemical productions through artificial photoenzymatic catalysis.
Optimizing Electronic and Geometrical Structure of Vanadium Doped Cobalt Phosphides for Enhanced Electrocatalytic Hydrogen Evolution
Authors: Wang Zumin , Meng Cheng , Wang Ji , Song Zhifan , Yu Ranbo 
Published: February 2023

AbstractDespite the expectation on transition‐metal phosphides as precious‐metal‐free electrocatalysts, the reported performance of these materials still necessitates further improvement. Ingenious regulations of both geometric and electronic structure have been proposed as an effective approach to boost their electrocatalytic properties. In this regard, the self‐supported V doped CoP nanowires on nickel foam are prepared to accommodate both optimized electronic structure and desired nanostructure, which enable large surface area, abundant active sites exposure, low charge transfer resistance, as well as favorable H* adsorption. As for the alkaline hydrogen evolution, it only requires a lower potential of 79 mV and 125 mV to drive 10 mA ⋅ cm−2 and 100 mA ⋅ cm−2 current with a Tafel slope of 47.41 mV ⋅ dec−1, which prevails over commercial Pt/C catalysts. The catalyst also exhibits excellent durability to retain activity unchanged for more than 16 h. Such a simple and convenient strategy by electronic tuning and structure design provides a new avenue toward the exploration of efficient electrocatalysts.
Investigations on Novel 1,3‐Diazetidine Based Four‐Membered N‐Heterocyclic Carbenes
Authors: Karl Leonard , Meisner Jan , Ganter Christian 
Published: February 2023

AbstractAttempts towards the synthesis of two novel four‐membered 1,3‐diazetidine based N‐heterocyclic carbenes (NHCs) containing an organic backbone with a carbonyl functionality were undertaken. These carbenes cannot be isolated but the respective carbene dimers are obtained in quantitative yield which undergo a degradation and rearrangement sequence upon thermal exposure. Some of the species involved in these thermal reactions could be isolated and characterized, others were observed by mass spectrometric experiments. Ab initio and density functional theory (DFT) calculations provide a mechanistic rationale for the experimental observations. Since dimerization is strongly favored, classic carbene trapping reactions remain a goal to achieve.
Stereoselective Domino Semipinacol‐Schmidt Reaction: Diastereoselective Synthesis of 7 a‐epi‐(+)‐Lepadiformine C and Formal Synthesis of 7 a‐epi‐(−)‐Lepadiformine A
Authors: Puppala Manohar , Gore Sangram , Eyong Kenneth O. , Baskaran Sundarababu 
Published: February 2023

AbstractA concise approach has been developed for the diastereoselective synthesis of the azatricyclic core of (−)‐7 a‐epi‐lepadiformine A (8 a) and (−)‐7 a‐epi‐lepadiformine C (8 b) using stereoselective domino semipinacol‐Schmidt reaction as a key step. In presence of TiCl4, the oxaspiropentane‐azide derivatives underwent stereoselective domino cyclization to furnish the corresponding angularly fused azatricyclic cores in very good yields. Moreover, azatricyclic core of (−)‐7 a‐epi‐lepadiformine A has also been realized, in a stepwise manner, through the intramolecular Schmidt reaction of azido‐spirocyclobutanone intermediate. The synthetic utility of domino semipinacol‐Schmidt reaction is further shown in the diastereoselective synthesis of (+)‐7 a‐epi‐lepadiformine C (7).
Photoacidity of Indolinospirobenzopyrans in Water
Authors: Berton Cesare , Pezzato Cristian 
Published: February 2023

AbstractThe reversible isomerism of indolinospirobenzopyrans is perhaps among the most studied phenomena in the field of molecular switches. Although they began to gain attention as early as 70 years ago following the seminal work of Hirshberg and Fischer, who were the first to recognize their photochromic behaviours, their implementation as photoacids emerged prominently only in the last decade. In this Review, we contextualize the prerequisites underlying the photo‐triggered proton release that occurs in these molecular switches, highlighting the most recent advances in their characterization and application as “metastable‐state photoacids” in water.
Pyrazolo[1,5‐a]pyrimidine‐Dioxaborinine Hybrid Dyes: Synthesis and Substituent Effect in the Photophysical Properties
Authors: Tigreros Alexis , Aranzazu Sandra‐L. , Ríos María‐C. , Portilla Jaime 
Published: February 2023

AbstractA novel family of pyrazolo[1,5‐a]pyrimidine‐dioxaborinine (PP‐DB) hybrid dyes was synthesized by the direct construction of the dioxaborinine (DB) fragment on the pyrazolo[1,5‐a]pyrimidine (PP) ring, which implies the formation of four new bonds in a one‐pot manner. The dyes’ optical properties were investigated and compared with the starting pyrazolo[1,5‐a]pyrimidines; a study evidencing large fluorescence quantum yields in products (φfup to 69 %) due to an intramolecular charge transfer (ICT) process from the PP core to a ring of DB (PP→DB) that is absent in precursors (φf=0.03–0.30). Time‐dependent density functional theory (TD‐DFT) calculations confirmed the fluorescence process involved in the novel dyes, where their ICT limits the non‐radiative process due to the restricted rotation in the D−A system. The present work provides insight into how phenyl and DB ring incorporation impact the optical properties of this new group of hybrids dyes based on PP‐DB.
Enantioselective Synthesis of [b]‐Annulated Azepane Scaffolds
Authors: Aeissen Enno , von Seggern Aaron R. , Schmidtmann Marc , Christoffers Jens 
Published: February 2023

AbstractCyclopenta‐, benzo‐, and cyclohepta[b]‐annulated azepane scaffolds were prepared in two steps from optically active cyclic α‐allyl‐β‐oxoesters. The first step was ruthenium‐catalyzed olefin cross metathesis with acrylonitrile. The second step was palladium‐catalyzed dihydrogenation which consists of three consecutive processes: The hydrogenation of the C−C double and C−N triple bonds was followed by the reductive amination via the iminium ion formed in situ from the primary amino function and the endocyclic carbonyl group. This last step gave, stereoselectively, the annulated azepanes with relative trans‐configuration. The amino function and the ester group define two points for further diversification of the scaffolds. The trifluoroacetyl derivatives allowed to establish the enantiopurity of the products to be 97–98 % ee by GLC on a chiral phase. The relative trans‐configurations and in one case also the absolute (R,R)‐configuration was established by X‐ray crystallography.
TbEu(BTC) as a dNTP label for LAMP outcome in nucleic acid testing
Authors: Gao Chenfeng , Wong Joseph J. Y. , Hall Elizabeth A. H. 
Published: February 2023

AbstractLanthanide metal organic frameworks (MOFs), Eu(BTC) and TbEu(BTC) were assessed to follow uptake of deoxynucleotide triphosphate (dNTPs) in loop mediated isothermal amplification (LAMP) using BST2.0. dNTPs could be determined below 150 μM. A ratiometic measurement of Tb3+:Eu3+ fluorescence in TbEu(BTC) gave a limit of detection of 2.08 μM for dNTPs. dNTP uptake slowed above 8 mM Mg2+. This correlated with end‐point and real‐time LAMP, using PicoGreen and SYBR green respectively. For the P.Mal‐Lau 18S rRNA primer set, with P. malariae a detection limit of 102.5 copies was obtained. Cross reactivity between P. malariae and P. knowlesi was shown with 18S rRNA primer sets.
Optimization and Comparison of Microwave-Assisted Extraction, Supercritical Fluid Extraction, and Eucalyptus Oil–Assisted Extraction of Polycyclic Aromatic Hydrocarbons from Soil and Sediment
Authors: Kariyawasam Thiloka , Doran Gregory S. , Howitt Julia A. , Prenzler Paul D. 
Published: February 2023

Abstract
Polycyclic aromatic hydrocarbons (PAHs) are persistent organic compounds of major concern that mainly accumulate in soils and sediments, and their extraction from environmental matrices remains a crucial step when determining the extent of contamination in soils and sediments. The objective of the present study was to compare the extraction of PAHs (phenanthrene, pyrene, chrysene, and benzo[a]pyrene) from spiked soil and sediment using supercritical fluid extraction (SFE) with ethanol as the modifier, microwave-assisted extraction (MAE), and eucalyptus oil–assisted extraction (EuAE). Recoveries of PAHs were comparable between the three methods, and &gt;80% of applied pyrene, chrysene and benzo[a]pyrene were recovered. The most efficient method of extracting PAHs from naturally incurred soils with different levels of contamination was SFE. A longer extraction time was required for the EuAE method compared with SFE and MAE under optimized conditions. However, EuAE required lower extraction temperatures (15–20 °C) compared with SFE (80 °C) and MAE (110–120 °C), and consumed less solvent than SFE and MAE. Compared with hexane/acetone used in MAE, the use of ethanol in SFE and eucalyptus oil in EuAE can be considered as more sustainable approaches to efficiently extract PAHs from spiked/naturally contaminated soils and sediments. And, although less efficient for matrices containing higher carbon content, EuAE offered a cheap, low-tech approach to extracting PAHs. Environ Toxicol Chem 2023;42:982–994. © 2023 The Authors. Environmental Toxicology and Chemistry published by Wiley Periodicals LLC on behalf of SETAC.