bluuebunny/crossref_metadata_2025_split huggingface dataset:
Unraveling the Therapeutic Potential of Menstrual blood-Derived Stromal Cells in Myocardial Infarction Management: A Computational Study
Authors: De Pedro M Á , Pulido M , Álvarez V , Marchena A M , Sánchez-Margallo F M , López E 
Published: January 2025

Abstract

Introduction
The treatment of myocardial infarction (MI) remains a challenge in cardiovascular medicine. While the combined use of Captopril, Bisoprolol, and Spironolactone is well-established in MI management, there is still a need to develop new therapeutic approaches. This study evaluates the therapeutic potential of the secretome derived from menstrual blood stromal cells (MenSCs) through in silico analysis, comparing its effects with those of the aforementioned drugs.

Methods
MenSCs (n=5) were cultured in DMEM + 1% ITS for 48h. The resulting conditioned medium was ultrafiltered (3 kDa) to obtain the secretome (S-MenSCs). Its content was analysed using transcriptomics and proteomics technologies. Bioinformatic analysis included the use of the STRING App to generate the interaction network (IN) of the drugs Captopril, Bisoprolol, and Spironolactone and their target genes, FunRich to identify MI-associated proteins interacting with these target genes, and miRTargetLink2.0 to predict interactions between miRNAs and drug targets. Finally, the S-MenSCs were compared with previously identified miRNAs and proteins, and an IN was generated using Cytoscape.

Results
Our findings reveal that S-MenSCs contain numerous miRNAs and proteins that interact with the target genes of the aforementioned drugs, replicating their effect in silico, especially that of Captopril and Spironolactone.

Conclusion
S-MenSCs exhibit an in silico therapeutic effect comparable to that of Captopril and Spironolactone, emerging as a potential therapy that simplifies MI management.
Evaluation of advanced therapies in severe post-myocardial infarction ventricular dysfunction: a swine model study
Authors: Vázquez F , Baez-Díaz C , Álvarez V , Torrescusa A , Blanco V , Pulido M , López E , Sánchez Margallo F M , Crisostomo V 
Published: January 2025

Abstract

Introduction
Ejection fraction (EF) is an important predictor of cardiovascular morbidity and mortality after myocardial infarction (MI). Advanced therapies (specifically cardiosphere-derived cells-CDCs- or their secretome –S-CDCs) could be useful in this scenario. Our objective was to evaluate whether intracoronary (IC) administration of CDCs or S-CDCs could improve cardiac function in a porcine model of severe post-MI ventricular dysfunction.

Methods
A reperfused MI model was induced in Large White pigs. Animals with an EF&lt;30% on day 7 post-MI received, in a blinded manner, 30x106 CDCs (n=7), 9.16 mg S-CDCs (n=6) or 5 ml of saline (n= 6) via IC route. Two magnetic resonance imaging studies were performed, on day 7 (to verify the inclusion criteria) and at 10 weeks, calculating: EF, indexed end systolic (ESVi) and diastolic (EDVi) volumes and infarct size.

Results
No significant differences in cardiac function were observed between the groups at 10 weeks. The S-CDCs group showed the best results in terms of cardiac function, with EF at 24±2% vs 29±3% vs 21±3%, EDVi at 104±8 ml/m² vs 95±8 ml/m² vs 107±9 ml/m², ESVi at 79±7 ml/m² vs 68±8 ml/m² vs 86±10 ml/m², and infarct size at 10±1% vs 11±2% vs 10±2% in the Control, S-CDCs, and CDCs groups, respectively.

Conclusion
In this experimental model of severe post-MI ventricular dysfunction, the administration of the assayed advanced therapies does not seem to be clinically advantageous, although the use of secretome could slightly improve overall ventricular function.
Identification of clinical needs for the improvement of ai-assisted colonoscopy cad systems
Authors: Blas Pagador J , Sánchez Peralta Luisa F , del Nozal Jorge Bernal , Fernández Hugo López , Rodríguez Alba Nogueira , Piñón Pedro Davila , Cubiella Joaquín 
Published: January 2025

Abstract

Introduction
Advances in artificial intelligence (AI)-assisted colonoscopy have enabled the detection and classification of polyps using computer-aided diagnosis (CAD) systems. However, there is still room for improvement, particularly in classification (CADx), and other clinical needs remain unaddressed.

Methods
To identify these needs, several meetings were held between expert groups with both clinical and technical backgrounds to recognise clinically relevant needs that are technically feasible.

Results
Some of the identified needs include: (i) identification of additional pathologies, such as diverticula or cancer invasion depth estimation; (ii) identification of the current location within the colon (including cecum identification); (iii) assessment of colon cleanliness and preparation quality (e.g., using the Boston Bowel Preparation Scale), both locally and globally; (iv) development of an alert system to guide explorations and avoid unexplored areas; and (v) automatic generation of colonoscopy reports, including a count of polyps found during the examination.

Conclusion
Several clinically feasible needs have been identified for the improvement of current AI-assisted CAD systems, which could further enhance the quality of examinations and optimise clinical workflows.
A novel cerebral ischemia-reperfusion model using intraluminal filament in rabbit
Authors: Luca Cava V , Sánchez Margallo F M , García Rico R , Martín Rosado H , Moreno Lobato B , Bote Chacón J , Picado Román N , Sun F 
Published: January 2025

Abstract

Introduction
Stroke models are based on permanent or transient occlusion of cerebral arteries. Despite transient occlusion model tends to produce more variable results, it can investigate reperfusion. Although ischemia-reperfusion model is well established in rat using a silicone-filament by exposition of carotid artery, the endovascular approach in large animal is still a challenge. The purpose was to develop a novel cerebral ischemia-reperfusion model in rabbit using an intraluminal wire by means of an endovascular approach and subsequent assessed by MRI.

Methods
Four New Zealand rabbits (4-5 kg) underwent cerebral ischaemia-reperfusion. A 4Fr introducer sheath was inserted into the femoral artery. Under fluoroscopy, a 4Fr Cobra catheter was placed into the common carotid artery. A 1.9Fr microcatheter was inserted coaxially into the internal carotid artery, and a 0.014” microwire was advanced into the middle cerebral artery (MCA). Alternatively, the posterior cerebral artery (PCA) was occluded through the vertebral artery when the above approach failed. After 1 hour, reperfusion was performed by withdrawing the microwire. MRI examinations were conducted from 1 to 3 hours later.

Results
Technical success was achieved in three out of four animals, with the MCA reached in two rabbits and the PCA in one. In the rabbit with technical failure, it was impossible to advance the microwire into either the MCA or PCA due to the tortuosity of the vessels and vasospasm.

Conclusion
Ischemia-reperfusion model in rabbit though an intraluminal wire may provide a new model using an endovascular approach for the earlier research of transient stroke.
Endovascular procedures for stroke in large animals: advantages and limitations
Authors: Luca Cava V , Sánchez Margallo Francisco M , García Rico R , Martín Rosado H , Sun F 
Published: January 2025

Abstract

Introduction
The translation of stroke research to clinical settings has been limited in recent decades. Large animal models offer advantages in translational stroke research, especially for the development of novel endovascular treatments. The species-specific cerebrovascular architecture can significantly influence the suitability of a stroke model. The purpose of this study was to assess the feasibility of the endovascular approach to the cerebral vasculature in large animal models.

Methods
Four different species were selected, including dogs, rabbits, pigs, and sheep. Angiographies of the common carotid artery were performed to visualize the internal carotid artery (ICA) and, subsequently, to access the cerebral vasculature. Additionally, angiographies of the vertebral arteries were conducted as an alternative approach. Catheterization of the cerebral vasculature was attempted in each species.

Results
The endovascular approach to the cerebral vasculature was successfully achieved in rabbits and dogs through catheterization of the ICA. In contrast, in pigs and sheep, this endovascular access was hindered by the rete mirabile, a vascular network that connects the ICA to the cerebral vasculature. Alternatively, the vertebral artery approach was attempted but failed to reach the basilar artery due to the tortuosity of the vessels.

Conclusion
The dog and rabbit models were suitable for cerebral endovascular approaches. However, the use of dogs is limited due to ethical considerations, while rabbits provide a viable alternative for neurointerventional procedures. In contrast, stroke models in pigs or sheep are only feasible with external surgical approaches, as the endovascular route was not achievable.
Multi-omics perspective: mechanisms of gastrointestinal injury repair
Authors: Zhao Haibin , Zhang Zhigang , Liu Hongyu , Ma Mingxiu , Sun Peng , Zhao Yang , Liu Xun 
Published: January 2025

Abstract
In this review, we examine the significance of multi-omics technologies in understanding the plethora of intricate processes that activate gastrointestinal (GI) injury repair. Multi-omics, which includes genomics, transcriptomics, proteomics, and metabolomics, allows intricate mapping of cellular responses and molecular pathways involved in GI repair. We highlight the potential of multi-omics to discover previously unknown therapeutic targets or elucidate the molecular basis of the pathogenesis of GI. Furthermore, we explore the possibilities of integrating omics data to improve prediction models, and summarize the state-of-the-art technological developments and persisting obstacles that hinder the translation of multi-omics into clinical practice. Finally, innovative multi-omics approaches that can improve patient outcomes and advance therapeutic strategies in GI medicine are discussed.
When the Hegemon Seeks Ontological Security: US Narratives on Rising Threats and the Future of the International Order
Authors: Tekin Beyza Çağatay , Tekin Rıfat Barış 
Published: January 2025

Abstract
This article examines strategic narratives in the United States’ 2022 National Security Strategy (NSS) through the lens of ontological security theory. The analysis suggests that the US is challenged in its ontological security by the current state of the international order and the rapid evolution of the global economy into an unfamiliar entity, largely as a result of the destabilising actions of geopolitical rivals. The NSS proposes substantial changes to routinised relationships and economic restructuring to alleviate ontological insecurity and maintain a sense of stability. This includes the global relocation of production activities, new mechanisms for regulating technological progress, investment, and trade, and increased state activism and interventionism. Strategic narratives legitimise change by emphasising the US’ role as a system builder dedicated to preserving and enhancing the resilience of the international order and ensuring that proposed transformations align with neoliberal economic governance and operate through indirect interventions. The NSS is engaged in reshuffling and shifting the focus of some of the biographical narratives that define the US’ self-identity, emphasising different aspects of its nature and history. Strategic narratives play upon emotions such as feelings of grandeur, entitlement, responsibility, and nostalgia to facilitate change and address ontological insecurity.
OP36 Lusvertikimab, a first-in-class IL7 receptor antagonist, in moderate to severe Ulcerative Colitis: results of a multicenter, randomized, placebo-controlled phase II study
Authors: Bourreille A , Kierkus J , Corallo F , Reinish W 
Published: January 2025

Abstract

Background
OSE127 lusvertikimab (L) is a first-in-class antagonist of IL-7R alpha chain developed for the treatment of ulcerative colitis (UC). The study objectives were to evaluate the efficacy and safety of L in patients (pts) with ulcerative colitis (UC) in a multicentre, randomised, double-blind, placebo-controlled phase 2 study CoTikiS (NCT04882007).

Methods
Adults with moderately to severely active UC (modified Mayo score [MMS] 4-9) and inadequate response to conventional and/or failure to advanced therapies were randomised 1:1:1 in 3 treatment groups (placebo, L450 mg or L850 mg) administered intravenously at week 0 (W0), W2 and W6. L450mg was dropped at a prespecified futility analysis after 58 patients. It was later showed not futile.
The primary endpoint (PE) was the comparison between groups in changes of the MMS from baseline at W10. Key secondary endpoints were clinical remission, endoscopic improvement, endoscopic remission and changes in endoscopic activity by the UC Endoscopic Index of Severity (UCEIS). Adverse events (AEs) and laboratory abnormalities were assessed for safety.

Results
There were no differences in demographic and disease characteristics between treatment groups (placebo n=49, L450 mg n=35 or L850 mg n=51). The PE was achieved: L450, L850 and pooled dose L450/L850 significantly reduced MMS at W10 as compared to placebo.
Key secondary endpoints were also improved by L450, L850 and pooled L450/L850 as compared to placebo. Significantly greater percentages of patients were in clinical remission (22%, 13.0% and 16.2% respectively vs 4.4% for placebo), had an endoscopic remission (34.7%, 19.4%, and 25% vs 12.6 % for placebo) and endoscopic improvement (44.6%, 24.3%, and 31.9% vs 12.6% for placebo) with L450. The UCEIS score was significantly improved with L450.
L450 and L850 were well tolerated. The most frequent adverse events for L450, L850 and placebo were lymphopenia (5.6%, 3.9% and 2% respectively), anaemia (2.8%, 0% and 4.1%), pyrexia (5.6%, 0%, 2%), UC exacerbation (2.8%, 3.9%, 6.1%) and Covid 19 (2.8%, 0%, 4.1%). Lymphopenia was of mild severity, transient and did not result in drug interruption, drug withdrawal or increased infection rate. No clinical or biological specific safety signal was reported.

Conclusion
Lusvertikimab, a pure IL-7 receptor antagonist, demonstrated its clinical and endoscopic efficacy in moderate to severe UC compared to placebo at week 10 with no clinically relevant safety signal. These positive findings support the further clinical development for treatment of UC to elaborate its potential place in the therapeutic armentarium of UC.
Invasive control and native restoration: Directing ecosystem transformation through purposeful food web manipulations
Authors: Mrnak Joseph T , Wilkinson Maxwel V , Sikora Logan W , Feucht Levi M , Mrnak Amber M , Vander Zanden M Jake , Sass Greg G 
Published: January 2025

ABSTRACT
Ecosystems are abruptly changing due to invasive species and global climate change. In lakes, invasive Rainbow Smelt Osmerus mordax can cause negative ecosystem effects through competitive and predatory interactions with native species leading to food web shifts away from native species dominance, altered zooplankton communities, and the decline or extirpation of native cool and coldwater fishes. We conducted two whole-lake removals of invasive Rainbow Smelt and simultaneous introductions of native Cisco Coregonus artedi through stocking. About 327 and 1.6 adult Rainbow Smelt/ha were removed and about 45 adult Cisco/ha were stocked over 4 years into the two experimental lakes. In one system, native Yellow Perch Perca flavscens relative abundance and density significantly increased by 556% and 143% post-manipulation, respectively. In the other system, native Walleye Sander vitreus relative abundance increased by 26% and became consistently present in the pelagic zone post-manipulation (allowing for density estimation). Rainbow Smelt relative abundance and density decreased by &gt;85% in both experimental lakes. The two ecosystems shifted to native species dominance while invasive Rainbow Smelt became insignificant components of the food webs. In these two intensive whole-lake manipulations, we applied the Resist–Accept–Direct (RAD) climate adaptation framework to test an applicable ecological adaptation strategy and used panarchy theory as an ecologically grounded pathway to purposefully direct ecosystem transformation. We used this holistic management framework to better understand and manage undesired ecological change—“food web thinking.” In the context of our study, two ecosystems were purposefully directed towards native food web structures, species interactions, and processes, which mitigated Rainbow Smelt driven negative effects.
Recovering the American horseshoe crab through a commitment to collaboration
Authors: Smith David R , Botton Mark L , Shin Paul K S 
Published: January 2025

ABSTRACT
American horseshoe crab Limulus polyphemus populations are recovering because of cooperation among diverse stakeholders and data-driven collective action. Limulus polyphemus is one of four extant species facing common threats, and conservation successes and limitations hold lessons applicable to all the species. We review the advancement in management and monitoring over recent decades, discuss the current population status throughout the species’ range, and describe the potential future status and recovery based on recent assessments. In retrospect, L. polyphemus conservation has followed the frameworks promoted by the International Union for the Conservation of Nature: mobilize networks to increase assessment capacity, engage diverse stakeholders, measure impacts, and amplify successes. Data show significantly increased populations in the Delaware Bay region and improved status in the Northeast. The average abundance of adults in Delaware Bay over the recent decade (2013–2022) is more than twice that in the previous decade (2003–2012). In 2022, the abundances for adult females and males in the Delaware Bay population were estimated to be 16 million and 40 million, respectively. However, reversing persistent poor conditions in some regions and mitigating the widespread threat of habitat loss from coastal development and sea level rise will rely on collaboration among diverse stakeholders to build upon the current conservation successes. Scientists and conservationists working on the horseshoe crab species indigenous to Asia are advancing along a similar track, establishing a monitoring network and mobilizing diverse stakeholders. However, there is a need for capacity building for robust assessment of the species in Asia to measure the impact of conservation, just as that need remains unmet for L. polyphemus in the Gulf of Mexico. The importance of partnerships and collaborations is apparent in their absence. The regions where partnerships and collaborations among researchers, conservationists, and agency scientists do not exist are those where the capacity for monitoring and assessment is notably lacking.
Healthy Aging and the Gut Microbiome in People With and Without HIV
Authors: Peters Brandilyn A , Xue Xiaonan , Hanna David B , Wang Yi , Wang Zheng , Sharma Anjali , Floris-Moore Michelle , Konkle-Parker Deborah , Alcaide Maria L , Sheth Anandi N , Topper Elizabeth F , Weber Kathleen M , Tien Phyllis C , Merenstein Daniel , Vásquez Elizabeth , Chen Yue , Mimiaga Matthew J , Stosor Valentina , Brown Todd T , Erlandson Kristine M , Dillon Stephanie M , Elsayed Noha S , Usyk Mykhaylo , Sollecito Christopher C , Kaplan Robert C , Burk Robert D , Qi Qibin 
Published: January 2025

Abstract

Background
Aging-related comorbidities are more common in people with human immunodeficiency virus (HIV) compared to people without HIV. The gut microbiome may play a role in healthy aging; however, this relationship remains unexplored in the context of HIV.

Methods
16S rRNA gene sequencing was conducted on stool from 1409 women (69% with HIV; 2304 samples) and 990 men (54% with HIV; 1008 samples) in the MACS/WIHS Combined Cohort Study. Associations of age with gut microbiome diversity, uniqueness, and genus-level abundance were examined in women and men separately, followed by examining relationships of aging-related genera with frailty (Fried frailty phenotype) and mortality risk (Veterans Aging Cohort Study [VACS] index).

Results
Older age was associated with greater microbiome diversity and uniqueness, greater abundance of Akkermansia and Streptococcus, and lower abundance of Prevotella and Faecalibacterium, among others; findings were generally consistent by sex and HIV status. An aging-related microbiome score, generated via combination of 18 age-related genera, significantly increased with age in both women and men independently of demographic, behavioral, and cardiometabolic factors. In general, age was more strongly related to microbiome features (eg, diversity, microbiome score) in men without compared to with HIV, but age-microbiome associations were similar in women with and without HIV. Some age-related genera associated with healthy/unhealthy aging, such as Faecalibacterium (related to reduced frailty) and Streptococcus (related to higher VACS index).

Conclusions
Age is associated with consistent changes in the gut microbiome in both women and men with or without HIV. Some aging-related microbiota are associated with aging-related declines in health.
Qualitative analysis of pain impact in adult patients with X-linked hypophosphatemia
Authors: Nishime Nicole , Theriault Christopher , Feinn Richard , Macica Carolyn M 
Published: January 2025

Abstract
We previously reported pain as a predominant finding in adult patients with XLH. This study explored health-related quality-of-life using the 36-item Short Form survey instrument (SF-36v1) and pain experiences of adults with X-linked hypophosphatemia (XLH) through qualitative analysis of interviews with 15 patients (11 females, 4 males). Age-adjusted differences using SF-36 were lower than the general population in all domains of health-related quality of life, with significant differences notably related to physical function and pain. Ten themes emerged from semi-structured interviews: 1) Chronic and Variable Pain: Patients reported persistent pain of varying intensity and location; 2) Impact on Mobility and Daily Life: Pain severely restricted daily activities, affecting lifestyle, employment, and independence; 3) Pain Management: Patients used medications, physical therapy, lifestyle changes, alternative therapies, and assistive devices; 4) Healthcare and Accessibility Challenges: Access to appropriate care and treatments was limited by insurance issues and healthcare providers’ lack of XLH knowledge; 5) Emotional and Psychological Impact: Chronic pain and limitations led to social isolation, depression, and emotional burden; 6) Desire for Improved Information and Support: Patients sought better information, treatment, and community support; 7) Challenges with Physical Inactivity: Staying active was essential but difficult, as inactivity worsened pain; 8) Navigating Healthcare Services: Accessing therapy and insurance was often challenging; 9) Long-term Outlook and Concerns: Patients worried about disease progression and future health; and 10) Advocacy and Awareness: Increased public and medical awareness was needed to improve care. Although pain is not a universal experience within this population, it is a significant issue for many individuals with XLH. These data illustrate the profound impact of XLH on multiple life aspects underscoring the need for effective pain management strategies, better healthcare accessibility, supportive tools, and a more knowledgeable medical community to improve quality of life. Insights from this study will guide the development of a digital pain self-management intervention tailored to XLH patients to address these needs.