bluuebunny/crossref_metadata_2025_split huggingface dataset:
Food as medicine, community as medicine: Mental health effects of a social care intervention
Authors: Thompson‐Lastad Ariana , Chiu Dorothy T. , Ruvalcaba Denise , Chen Wei‐Ting , Tester June , Xiao Lan , Emmert‐Aronson Benjamin O. , Chen Steven , Rosas Lisa G. 
Published: January 2025

AbstractObjectiveTo assess mental health related outcomes of Recipe4Health, a multisectoral social care partnership implementing produce prescriptions with or without group medical visits (GMVs).Study Setting and DesignRecipe4Health was implemented at five community health centers from 2020 to 2023. Primary care teams referred patients with food insecurity and/or nutrition‐sensitive chronic conditions (e.g., diabetes, depression) to 16 weeks of Food Farmacy (produce prescriptions) with the option of GMV participation. We used a convergent mixed‐methods design including survey and interview data.Data Sources and Analytic SampleWe conducted (1) participant surveys pre‐ and post‐intervention and (2) semi‐structured interviews with Recipe4Health participants and partner organization staff. Linear mixed effects models examined changes in mental health and related outcomes. Interviews were analyzed using codebook thematic analysis.Principal FindingsProgram participants were middle‐aged, primarily women, and from diverse racial/ethnic backgrounds (majority Latine and Black). At baseline, moderate or severe depression and/or anxiety symptoms were reported by 77/188 (41%) of Food Farmacy‐only participants, and 113/284 (40%) of Food Farmacy +GMV participants. Among Food Farmacy‐only participants, post‐intervention depression and anxiety symptoms significantly improved only among those who did not have baseline depression/anxiety (PHQ9: −1.7 [95% CI: −2.8, −0.6]; GAD7: −1.8 [95% CI: −2.9, −0.8]). Among Food Farmacy +GMV participants, mental health symptoms improved regardless of baseline mental health; among those with baseline depression/anxiety: PHQ9: −2.4 (95% CI: −3.6, −1.2); GAD7: −0.9 (95% CI: −2.0, 0.1); among those without: PHQ9: −2.2 (95% CI: −3.2, −1.2); GAD7: −2.2 (95% CI: −3.1, −1.2). Improvements in social needs (food insecurity, loneliness) and health‐related behaviors (fruit/vegetable intake, physical activity) varied by intervention arm and baseline depression/anxiety symptom level. In interviews, staff and patients endorsed produce prescriptions for improving nutrition and food insecurity, and GMVs for increasing social support.ConclusionSocial care interventions providing vegetables and fruit, with or without group medical visits, may concurrently address mental health symptoms and social needs.
An aquaria set‐up for long‐term, multiple‐stressor research in marine organisms
Authors: Gutiérrez‐Zárate Cristina , Veiga Alfredo , Gori Andrea , Gerpe Fabián , Domínguez Leo , Rodríguez Alberto , Arias Noelia , Álvarez Marta , Acerbi Rubén , Vázquez Lucía , Movilla Juancho , Varela Marta M. , Alba‐Salgueiro Rodrigo , Valencia‐Vila Joaquín , Orejas Covadonga 
Published: January 2025

Abstract

Multiple stressor research in aquaria is a useful approach to better understand the ecophysiology of marine species under different environmental conditions, including global change scenarios. Long‐term experiments are helpful to detect the response of sustained exposure to selected environmental conditions. Here, we present an experimental set‐up suitable to run long‐term experiments, composed of a life support system, a cost‐effective aquaria set‐up and an open‐source controller based on the use of a Raspberry Pi. In this set‐up, temperature, pH and dissolved oxygen (DO) are individually manipulated and simultaneously controlled in eight different treatments.

To prove the efficacy of the set‐up, we provide an assessment over a nine‐month experiment on a deep‐sea coral species, combining values from current in situ and IPCC AR5 RCP 8.5 scenarios for the aforementioned parameters. Recorded data from the controllers and independent measurements (e.g. cross‐checking with portable multiparameter devices and laboratory analyses) throughout the experimental time have been analysed and results have been discussed.

Overall, the experimental set‐up performed well, proving the stability of the parameters over time, both individually and in combination. On average, low and high‐temperature treatments varied ~0.4 and 0.3°C, respectively. Low pH treatments were maintained within 0.05 pH units, whereas ambient pH treatments varied ~0.04 pH units. Low DO treatments had a variation of ~0.3 mg L−1, and ambient DO treatments varied ~0.2 mg L−1. No significant differences between scenarios for any parameter were detected (p < 0.05). The resulting programming code to read, control and register the values for these parameters is provided to contribute to its replicability across institutions.

The set‐up performed well over extensive periods while providing a customisable controller as a cost‐effective alternative. The versatility of the system, allowing to work with different species, environments and scenarios makes it valuable for aquaria experiments where interactions of multiple environmental factors need to be tested.
Aging disrupts locus coeruleus‐driven norepinephrine transmission in the prefrontal cortex: Implications for cognitive and motor decline
Authors: Budygin Evgeny , Grinevich Valentina , Wang Zhong‐Min , Messi María Laura , Meeker William Ryan , Zhang Jie , Stewart William Matthew , Milligan Carol , Delbono Osvaldo 
Published: January 2025

AbstractThe locus coeruleus (LC)‐prefrontal cortex (PFC) circuitry is crucial for cognition, planning, posture and mobility. This study examines the role of norepinephrine (NE) in elucidating the neurobiological basis of age‐related cognitive and motor declines. Aged mice exhibited reduced spatial learning, impaired memory, decreased physical endurance, and notable changes in locomotor behavior. The neurochemical foundations of these deficits were investigated through fast‐scan cyclic voltammetry to measure NE release in the PFC and LC, both in vivo and in brain slices. Additionally, oxygen levels were monitored as a proxy for PFC neuronal function, and NE levels were analyzed in the extracellular space via microdialysis and total content in the PFC. Aged mice exhibited a frequency‐dependent increase in NE release in the PFC upon LC stimulation, suggesting alterations in neural responsiveness due to aging. We also recorded slower NE reuptake rates and increased NE content and neuronal activity, indicated by higher oxygen levels and facilitated neuron activation due to membrane depolarization recorded via whole‐cell patch‐clamp. To understand the basis for LC‐driven NE surges in the PFC with aging, we examined the expression levels of two proteins critical for presynaptic NE release and NE reuptake: the α2a‐adrenergic receptor and the NE transporter. Both showed a significant decrease in the PFC with aging. These findings support the concept that aging significantly alters the structural and functional dynamics within the LC‐PFC neural circuit, impacting NE modulation and neuronal activity, which may underlie the observed declines in cognitive and motor functions in aging populations.
Does cyber dating abuse victimization predict next‐day alcohol and cannabis use among college students?
Authors: Brem Meagan J. , Shaw T. J. , Tobar‐Santamaria Allison 
Published: January 2025

AbstractBackground and ObjectivesCyber dating abuse (CDA) is prevalent on college campuses, with 43% of college students experiencing CDA each year. Yet, the potential impacts of CDA victimization on college students' health outcomes remain poorly understood. Informed by the self‐medication hypothesis and longitudinal data linking dating abuse to substance use outcomes, the present study tested the hypothesis that CDA victimization positively associates with college students' next‐day alcohol use (number of drinks consumed, odds of any drinking) and odds of cannabis use (yes/no). We also explored whether CDA's association with next‐day substance use varied as a function of gender.MethodsDaily data on alcohol use, cannabis use, and CDA victimization were collected across 60 consecutive days from 236 undergraduates who were in dating relationships (73.73% cisgender women).ResultsMultilevel modeling revealed gender differences. Among men, CDA victimization predicted next‐day cannabis use (aOR = 7.34, p < .001), but negatively related to the number of drinks consumed the following day (B = −2.63, p < .001). Among women, CDA victimization did not relate to next‐day cannabis or alcohol use. Regardless of gender, CDA victimization was unrelated to the odds of any drinking.Discussion and ConclusionsCollege men are more likely to use cannabis the day after experiencing CDA victimization relative to other days. Targeting CDA and related aftermath may benefit college‐based substance use intervention.Scientific SignificanceThis is the first study to identify that cannabis use occurs subsequent to CDA victimization, which can inform college‐based CDA and cannabis use prevention programming.
Recurrence‐free survival after hepatectomy using propofol‐based total intravenous anaesthesia and sevoflurane‐based inhalational anaesthesia: a randomised controlled study
Authors: Kwon Ji‐Hye , Kim Jeayoun , Yeo Hyean , Kim Keoungah , Rhu Jinsoo , Choi Gyu‐Seong , Kim Jongman , Joh Jae‐Won , Kim Kyunga , Kim Min‐Ji , Jeong Ji Seon , Lee Jong‐Hwan , Han Sangbin , Ko Justin S. , Gwak Mi Sook , Kim Gaab Soo 
Published: January 2025

SummaryBackgroundWhile evidence from preclinical and observational cohort studies have suggested potential disparities in tumour behaviour associated with the choice of intra‐operative anaesthetics, clinical evidence of tumour recurrence and metastasis remains inconclusive. We aimed to compare the impact of intra‐operative anaesthesia on oncologic outcomes following hepatectomy for hepatocellular carcinoma.MethodsAdult patients scheduled for elective hepatectomy for hepatocellular carcinoma were assigned randomly (1:1) to either propofol‐based total intravenous anaesthesia or sevoflurane‐based inhalational anaesthesia. For recurrence‐free survival, overall survival, intrahepatic recurrence‐free survival and extrahepatic recurrence‐free survival, the survival curves of the two groups were estimated using the Kaplan–Meier method and compared with the log‐rank test. The primary outcome was one‐year recurrence‐free survival.ResultsAmong the 536 patients enrolled, primary analysis comprised 228 and 226 patients in the total intravenous anaesthesia and sevoflurane‐based inhalational anaesthesia groups, respectively. The probability of recurrence‐free survival at one year was 79.1% (47 events) and 77.7% (50 events) in the total intravenous anaesthesia and sevoflurane‐based inhalational anaesthesia groups, respectively (adjusted hazard ratio 1.04, 95%CI 0.72–1.52). The probability of intrahepatic and extrahepatic recurrence‐free survival, as well as overall survival at one year, was not significantly different between total intravenous anaesthesia and sevoflurane‐based inhalational anaesthesia: 81.3% (42 events) vs. 81.7% (41 events); 91.5% (19 events) vs. 88.8% (25 events); 99.1% (2 events) vs. 100.0% (no event), respectively. Subgroup analyses revealed that in patients undergoing open hepatectomy, total intravenous anaesthesia was associated with a significantly lower hazard of tumour recurrence or death (hazard ratio 0.49, 95%CI 0.25–0.95, p = 0.034), while in patients undergoing laparoscopic surgery, no significant difference was observed (hazard ratio 1.14, 95%CI 0.73–1.80, p = 0.558).DiscussionIntra‐operative anaesthesia technique did not affect postoperative recurrence and overall survival in patients with hepatocellular carcinoma undergoing hepatectomy.
Distinct subtypes of post‐transplant lymphoproliferative disorders: CHIP‐like mutations in early lesions and substantial mutational differences between EBV‐positive and EBV‐negative diffuse large B‐cell lymphomas
Authors: Ivanova Vanesa‐Sindi , Menter Thomas , Cui Ningxuan , Leary Peter , Zinner Carl , Halter Jörg P. , Stenner Frank , Dirnhofer Stefan , Müller Anne , Tzankov Alexandar 
Published: January 2025

SummaryPost‐transplant lymphoproliferative disorders (PTLD) and lymphomas in immunocompromised individuals represent significant clinical challenges, with a limited understanding of their pathogenesis. We investigated a PTLD cohort (n = 50) consisting of ‘early lesions’ (infectious mononucleosis‐like PTLD, plasmacytic and follicular hyperplasias), polymorphic PTLD and post‐transplant diffuse large B‐cell lymphomas (PT‐DLBCL). The study also included 15 DLBCL with autoimmune/immunocompromised backgrounds (IS‐DLBCL) and 14 DLBCL, not otherwise specified (DLBCL, NOS), as control. To investigate microarchitectural and genetic changes, immunohistochemistry, multiplex immunofluorescence (mIF), fluorescence in situ hybridisation and high‐throughput sequencing were performed. Scarcity of viral infections other than Epstein–Barr virus (EBV) was observed. mIF revealed lower Treg infiltration in PT‐DLBCL and high CD8+/PD1+ T cells in IS‐DLBCL. MYC rearrangements were most common in PT‐DLBCL, followed by IS‐DLBCL and DLBCL, NOS, all EBV‐negative. TP53 mutations were frequent in EBV‐negative PT‐DLBCL and DLBCL, NOS but absent in ‘early lesions’. NOTCH1 mutations were predominant in PT‐DLBCL (N1 DLBCL‐subgroup). Gene expression profiling showed a significant overlap between ‘early lesions’ and polymorphic PTLD. The presence of clonal haematopoiesis of indeterminate potential (CHIP)‐like mutations and the absence of immune‐escape gene mutations in ‘early lesions’ suggest these disorders may represent clonal expansions driven by exogenic immunosuppression and/or EBV infection ‘substituting’ for mutations of the latter group of genes.
Diagnosis and management of monoclonal gammopathy of renal significance: A British Society for Haematology good practice paper
Authors: Pinney Jennifer , Roufosse Candice , Kousios Andreas , Chaidos Aristeidis , Gillmore Julian D. , Rainone Francesco , Choudhuri Satarupa , Ramasamy Karthik , Blakey Sarah , Ashcroft John , Chan Y. L. Tracey , Cockwell Paul , Pratt Guy ,   the BSH Committee
Published: January 2025

SummaryThis guideline provides consensus opinion on the investigations required for people presenting with suspected monoclonal gammopathy of renal significance to both nephrology and haematology physicians. The guideline discusses the principles of treating a patient with MGRS and provides recommendations for both supportive management and haematological therapy. It details the recommended on‐going monitoring required for both specialty areas.
Characteristics of refractory disease and persistent symptoms in inflammatory arthritis: Qualitative framework analysis of interviews with patients and health care professionals
Authors: Chaplin Hema , Simpson Carol , Wilkins Kate , Meehan Jessica , Ng Nora , Galloway James , Scott Ian C. , Sen Debajit , Tattersall Rachel , Moss‐Morris Rona , Lempp Heidi , Norton Sam 
Published: January 2025

AbstractObjectivesThis study aims to explore patients' and clinicians' understanding and experiences of refractory disease (RD) and persistent physical and emotional symptoms (PPES) in patients with inflammatory arthritis (IA), namely rheumatoid arthritis or polyarticular juvenile idiopathic arthritis from their perspectives through interviews and/or focus groups.DesignA qualitative study was conducted, following a pragmatic epistemology approach with framework analysis employed.MethodsSemi‐structured interviews or focus groups with IA patients (n = 25) and multi‐disciplinary rheumatology HCPs (n = 32) were conducted at one time point to obtain participants respective understanding and experiences of managing RD/PPES, and its impact on the patient‐professional relationship.ResultsThree key themes were identified from both patients and professionals' experiences of RD/PPES: (1) relevant treatment experiences, (2) symptoms (with or without inflammation) and (3) impact: physical, psychological and social. These themes included 28 specific categories that would be considered as components characterizing RD/PPES, most common to both patients and HCPs with six being patient‐specific and only one HCP‐specific. The specific biopsychosocial symptoms and impacts of RD/PPES pertain to pain, fatigue, stiffness, joint involvement and physical, psychological and social functioning and quality of life, covering disease‐related distress, mobility and independence. Wider influential factors such as comorbidities, non‐adherence, health/medication beliefs and behaviours and social support were also identified.ConclusionCommon persistent symptoms that have both mental and physical impact characterize RD/PPES in IA and therefore a more integrated holistic approach to treatment is needed from multi‐disciplinary HCPs, including health psychologists.
Rnf40 Exacerbates Hypertension‐Induced Cerebrovascular Endothelial Barrier Dysfunction by Ubiquitination and Degradation of Parkin
Authors: Kou Chengkun , Zhao Xu , Fan Xin , Sun Runmin , Wang Wenting , Qi Miaomiao , Zhu Lulu , Lin Xin , Yu Jing 
Published: January 2025

ABSTRACTAimsWe aimed to investigate the role of Rnf40 in hypertension‐induced cerebrovascular endothelial barrier dysfunction and cognitive impairment.MethodsWe employed microarray data analysis and integrated bioinformatics databases to identify a novel E3 ligase, Rnf40, that targets Parkin. To understand the role of RNF40 in hypertension‐induced cerebrovascular endothelial cell damage, we used pAAV‐hFLT1‐MCS‐EGFP‐3×Flag‐mir30shRnf40 to establish an Rnf40‐deficient model in spontaneously hypertensive rats (SHRs). We also evaluated the cerebrovascular endothelial barrier function, cerebral blood flow, and cognitive performance.ResultsWe observed reduced mitophagy in cerebrovascular endothelial cells of SHRs compared with that in Wistar‐Kyoto rats. Rnf40 facilitated K48‐linked polyubiquitination and degradation of Parkin, thereby inhibiting mitophagy. In the Rnf40‐deficient SHR model, knocking down Rnf40 restored mitophagy in cerebrovascular endothelial cells. Additionally, levels of tight junction proteins and cerebrovascular endothelial barrier function improved following Rnf40 downregulation. Rnf40 depletion also improved global cognitive performance and restored cerebral blood flow in SHRs.ConclusionOur findings suggest that increased Rnf40 levels exacerbate hypertension‐induced cerebrovascular endothelial barrier dysfunction by ubiquitinating Parkin.
Amyloidosis and Heart Transplantation in a New Era
Authors: Lyle Melissa A. , Farina Juan Maria M. , Wiedmeier‐Nutor Erin , Lindpere Vanda , Klanderman Molly , Nativi‐Nicolau Jose N. , Leoni Moreno Juan Carlos , Goswami Rohan M. , Yip Daniel S. , Patel Parag C. , Sher Taimur , Rosenbaum Andrew N. , AbouEzzeddine Omar F. , Boilson Barry A. , Kushwaha Sudhir S. , Clavell Alfredo L. , Steidley David E. , Hardaway Brian W. , Scott Robert L. , LeMond Lisa M. , Fonseca Rafael , Gertz Morie A. , Dispenzieri Angela , Grogan Martha , Rosenthal Julie L. 
Published: January 2025

AbstractBackgroundThe prognosis in patients with advanced cardiac amyloidosis (CA) remains poor.ObjectivesWe sought to describe survival post heart transplantation (HT) in amyloid compared with non‐amyloid recipients, highlight waitlist times within the new allocation system across three Organ Procurement and Transplantation Network (OPTN) regions, and describe multiorgan transplantation (MOT) in hereditary amyloidosis.MethodsThis is a retrospective review of end‐stage CA patients who underwent HT at Mayo Clinic from January 2007 to December 2020. Wait time was compared in the new versus old OPTN allocation era starting December 18, 2018 by Wilcoxon rank sum test. All‐cause mortality for those with and without CA was compared using Kaplan–Meier estimates with log rank analysis, censoring December 16, 2022.ResultsFifty‐five patients with CA underwent HT between 2007 and 2020, 8 light chain amyloidosis (AL) (14.5%), 28 hereditary transthyretin (ATTRv) (50.9%), 17 wildtype transthyretin (ATTRwt) (30.9%), and 2 hereditary apolipoprotein A1 (AApoA1) amyloidosis patients (3.6%). No significant difference in overall survival post‐transplant was seen in amyloid compared with non‐amyloid (p = 0.816). Median time to HT was shorter in the new system, 45 days (IQR 24, 78) versus 174 days (IQR 76.5, 483.5), p = 0.006. There was a decline in MOT in hereditary amyloidosis over time with the concomitant rise in disease‐targeted therapies.ConclusionsHT survival in CA patients was similar to non‐amyloid patients. The new allocation system benefits this cohort with shorter wait times. There is less MOT in hereditary amyloidosis with increased utilization of disease‐targeted therapy.
Effect of Cdk1 gene disruption on cell cycle progression in newt cells
Authors: Nakao Yuta , Okamoto Kazuko , Tazawa Ichiro , Nishijima Tatsuro , Furuno Nobuaki , Sakuma Tetsushi , Yamamoto Takashi , Takeuchi Takashi , Hayashi Toshinori 
Published: January 2025

AbstractCyclin‐dependent kinases (CDKs) are key regulators of cell cycle progression, in conjunction with cyclins. The cyclin‐CDK system is highly conserved among eukaryotes, and CDK1 is considered essential for progression through the M phase. However, the extent to which cell cycle progression depends on CDK1 varies between cell types. Therefore, a range of cell types must be analyzed to comprehensively elucidate the role of CDK1. Cdk1‐knockout mice exhibit lethality at an early developmental stage, specifically before the differentiation of various cell types. The aim of the present study was to characterize the effects of CDK1 deficiency in amphibian newts. Cdk1 was disrupted by injecting fertilized newt eggs with CRISPR/Cas9, and the resulting effects on embryonic development and cell proliferation were then evaluated. In both wild‐type and Cdk1‐crispant newt embryos, CDK1 protein was either stored in the egg until late embryogenesis or potentially derived from maternal mRNA, which may also be stored during this period. The embryos survived to the hatching stage, during which the cells responsible for forming the basic organs differentiated. To further characterize the long‐term effects of Cdk1 knockout, parabiosis experiments were conducted using wild‐type embryos and Cdk1 crispants. The results suggested that an endocycle occurred in the crispant larvae, as evidenced by increases in the size of several types of cells. It is anticipated that studies using newts will provide further insights into the role of Cdk1 in regulating the cell cycle.
Double Disadvantage? Internal Migration, Gender and Labour Market Outcomes Among Recent College Graduates in China
Authors: Zhao Mengyao 
Published: January 2025

ABSTRACTThis study examines three waves of data from a nationally representative survey, the China College Student Survey (2010, 2013 and 2015), to determine whether highly educated female graduates who choose to move outside their hukou‐registered cities experience a double‐negative effect in terms of initial earnings attainment and work organisation entry due to their gender and migrant status in China's urban labour market. The results from multinomial logistic regression and OLS models show that, on the one hand, female graduate migrants are less likely to enter government organisations that afford institutional protection from gender discrimination. On the other hand, female graduate migrants are paid significantly less than their male counterparts in the graduate labour market. Therefore, in China's urban labour market, female graduates suffer a double‐negative effect on earnings attainment and work organisation entry. This study extends ‘the double‐disadvantage thesis’ to the study of internal migration, contributing to existing knowledge by showing that the interaction between gender and migration produces different labour market outcomes among migrant groups.