bluuebunny/crossref_metadata_2025_split huggingface dataset:
Prevalence of Plasmodium sp. infection on endemic primates in the Buton Utara Wildlife Sanctuary, Southeast Sulawesi, Indonesia
Authors: Lempang Meyby Eka Putri , Permana Dendi Hadi , Asih Puji Budi Setia , Wangsamuda Suradi , Dewayanti Farahana Kresno , Rozi Ismail Ekoprayitno , Setiadi Wuryantari , Syahrani Lepa , Muslimin Lucia , Malaka Ratmawati , Syafruddin DIn 
Published: August 2023

Abstract
Background
The recent emergence of zoonotic malaria in many parts of Indonesia alerts the need to establish mitigation efforts to prevent and contain the cases. The archipelago of Indonesia is home to many species of non-human primates (NHPs), and massive deforestation has substantially reduced the NHPs' habitat and intensified the interaction between NHPs and humans. The present study aims to determine the malaria prevalence among the NHPs in the Buton Utara Wildlife Sanctuary (BUWS) and the risk of zoonotic malaria among the human inhabitant adjacent to the site.
Methods
Epidemiologic surveys were conducted to determine the prevalence of malaria on endemic primates inhabiting BUWS, Indonesia, between 2020 and 2021 through the capture and release of NHPs. Traps were installed in several localities surrounding the sanctuary that border the human settlement. The captured primate was anesthetized, and blood samples were aseptically drawn using phlebotomy to make a blood smear and dried blood spot (DBS) on filter paper.
Results
Analysis of the DNA extracted from the DBS revealed that ten of the 26 captured primates were infected with Plasmodium inui, 2 with P. cynomolgi, and 1 with P. simiovale. Molecular analysis on the primate species caught indicated that all were macaques and originated from one species, Macaca brunnescens as evidenced by the barcoding DNA markers using mitochondrial DNA (mtDNA) Cytochrome Oxidase Subunit I (COI) and internal transcribed spacer 2 (ITS2) of the nuclear ribosomal DNA.
Conclusions
Overall, the findings indicate a high prevalence of malaria among NHPs in the wildlife sanctuary forest and deserve further surveillance to prevent spill-over to the human inhabitant surrounding the sanctuary.
Assessment and analysis of tree damage caused by forest road construction in a scotch pine stand: A case study from Alabarda/Bolu-Türkiye
Authors: Türk Yılmaz , Bodur Mücahit 
Published: August 2023

Abstract
The aim of this study is to determine the forest road construction area and environmental impact area, and to determine the tree injuries caused by forest road construction, the filling area covering the stand, and the distances of the trees directly affected by the tree injuries from the road axis. In this direction, 700 meters of the forest road numbered 194 within the boundaries of Alabarda Forest Management Sub-Directorate (Bolu/Turkey) was selected as the study area. The forest road environmental impact area was found by determining the rolling boundary of the backfill material with the differential global positioning system (DGPS) device. In addition, the position of the trees in this area in relation to the road and their damage conditions were determined and the wound sizes were measured. As a result of the study, the road construction impact area was 46479 m², the average spread distance of the backfill material was 50.34 m, the average distance of the damaged trees from the road was 28.25 m, and the average distance of the trees that were not damaged was 35.52 m. In addition, a total of 1477 trees were identified in the construction impact area and 427 of these trees were damaged. According to the results of the statistical analyses, a statistically significant positive correlation was determined between bark wound area and diameter. In addition, a statistically significant negative correlation was determined between the distance of the trees from the road axis and the wound area and the height of the backfill.
Enhancing Embedded Systems Development with TS-
Authors: Yu Xingzi , Tang Wei , Xiong Tianlei , Chen Wengang , He Jie , Yang Bin , Qi Zhengwei 
Published: August 2023

Abstract
The lack of flexibility and safety in C language development has been criti- cized for a long time, causing detriments to the development cycle and software quality in the embedded systems domain. TypeScript, as an optionally-typed dynamic language, offers the flexibility and safety that developers desire. With the advancement of Ahead-of-Time (AOT) compilation technologies for TypeScript and JavaScript, it has become feasible to write embedded applications using TypeScript. Despite the availability of writing AOT compiled programs with TypeScript, implementing a compiler toolchain for this purpose requires substantial effort.
To simplify the design of languages and compilers, this paper presents a new compiler toolchain design methodology called TS−, which advocates the generation of target intermediate language code (such as C) from TypeScript rather than the construction of higher-level compiler tools and type systems on top of the intermediate language. TS− not only simplifies the design of the system but also provides developers with a quasi-native TypeScript development experience. This paper also presents Ts2Wasm, a prototype that implements TS− and allows compiling a subset of language TypeScript to WebAssembly (WASM). The tests from the repository TypeScript show that Ts2Wasm provides 3.8x as many features compared to the intermediate language (AssemblyScript). Regarding performance, Ts2Wasm offers a significant speed-up of 1.4x to 19x. Meanwhile, it imposes over 65% less memory overhead compared to Node.js in most cases.
Tracking the first electron transfer step at the donor side of oxygen-evolving photosystem II by time-resolved infrared spectroscopy
Authors: Dekmak Mohamad Yahia , Mäusle Sarah M. , Brandhorst Janosch , Simon Philipp S. , Dau Holger 
Published: August 2023

Abstract

In oxygen-evolving photosystem II (PSII), the multi-phasic electron transfer from a redox-active tyrosine residue (TyrZ) to a chlorophyll cation radical (P680+) precedes the water-oxidation chemistry of the S-state cycle of the Mn4Ca cluster. Here we investigate these early events, observable within about 10 nanoseconds to 10 microseconds after laser-flash excitation, by time-resolved single-frequency infrared (IR) spectroscopy in the spectral range of 1310–1890 cm− 1 for oxygen-evolving PSII membrane particles from spinach. Comparing the IR difference spectra at 80 ns, 500 ns, and 10 µs allowed for the identification of quinone, P680 and TyrZ contributions. A broad electronic absorption band assignable P680+ was used to trace specifically the P680+ reduction kinetics. The experimental time resolution was taken into account in least-square fits of P680+ transients with a sum of four exponentials, revealing two nanosecond phases (30–46 ns and 690–1110 ns) and two microsecond phases (4.5–8.3 µs and 42 µs), which mostly exhibit a clear S-state dependence, in agreement with results obtained by other methods. Our investigation paves the road for further insight in the early events associated with TyrZ oxidation and their role in the preparing the PSII donor side for the subsequent water oxidation chemistry.
Computation of the masses of the elementary particles
Authors: Mashford John 
Published: August 2023

Abstract
An approach to gauge theory in the context of locally conformally flat space-time is described. It is discussed how there are a number of natural principal bundles associated with any given locally conformally flat space-time X. The simplest of these principal bundles is the bundle PG(X) with structure group G = U(2,2). An 11 dimensional bundle Q with structure group a certain 7 dimensional group K is constructed by a method involving a reduction of structure group for the bundle PG(X). It is shown how the gauge groups U(1), SU(2) and SU(3) can be derived from the geometry of locally conformally flat space-time. Fock spaces of multiparticle states for the fields of the standard model are constructed in the context of bundles with these groups as structure groups. Scattering and other particle interaction processes are defined in terms of linear maps between multiparticle state spaces. A technique for computing analytically and/or computationally the masses of the elementary particles is described. This method involves the computation of a certain quantity called the integral mass spectrum for a given family of particles and then the masses of the particles in the family are determined to be the locations of the peaks of the integral mass spectrum. The method is applied successfully to the cases of the charged leptons and the Z0 boson.
Desenvolvimento de Soluções SIG para a Administração Pública Brasileira – Uma Revisão Sistemática
Authors: Teixeira de Oliveira Filho José Antonio , Frata Furlan Peres Fabiana 
Published: August 2023

Os Sistemas de Informações Geográficas têm evoluído nos últimos trinta anos, seguindo os avanços de demais tecnologias e se tornando ferramenta fundamental para diversas tarefas, tanto na iniciativa privada quanto no setor público. As características de coletar, armazenar, recuperar e disseminar dados espaciais são de grande utilidade para o desenvolvimento de ações no setor público onde, não raramente, dificuldades como escassez de investimento e de mão de obra se apresentam como desafios a serem superados. Este artigo apresenta uma revisão sistemática da literatura identificando a produção científica entre 2018 e 2022, sobre Sistemas de Informações Geográficas desenvolvidos para o serviço público brasileiro, seus principais atores e campos de aplicação. Para realizar as buscas, foram consideradas as bases de dados indexadas IEEE Digital Library, ISI Web of Science, Science Direct e Scopus. As buscas retornaram 263 artigos, dos quais 79 eram duplicados e 171 não atendiam aos critérios de inclusão estabelecidos. Desse modo restaram 13 artigos para análise. Os resultados possibilitaram avaliar a popularização desses sistemas no âmbito governamental e revelaram os principais campos de aplicação, os softwares mais utilizados e as outras características a respeito das soluções desenvolvidas no período.
Reconsidering the Role of Fatigue Irregular Testing in Scientific Conclusions About the Fatigue Limit
Authors: Gadolina I. V. 
Published: August 2023

Aim. We suggest a method of using the fatigue test in case of irregular loading for studying the key subject matters related to material fatigue, specifically those to do with fatigue limit. It is important to know the fatigue limit characteristic in order to speed up tests; in order to ensure stable and reliable servicing of machines.Methods. Comparative examinations in cases of block and random loadings enable researchers to make corresponding conclusions without excessively extending the duration of tests and without recurring to supersonic frequency tests that are to a certain degree controversial. An example of applying the proposed method is given. The second stage of fatigue, specifically the crack propagation stage, is also considered. The key idea is similar. Testing under irregular loading allows making conclusions regarding the maximum among small amplitudes that do not cause damage.Conclusions. The proposed method allows using special testing methods to obtain information that is valuable to engineers and researchers. The tests are also closer to the field conditions of machinery operation.
An intelligent system for constructing metro train driver working schedules
Authors: Markevich A. V. , Sidorenko V. G. 
Published: August 2023

Aim. The paper aims to tests and analyse the results of application of an intelligent system for constructing metro train driver working schedules that is intended for automatically improving the efficiency of utilisation of the working time of metro train drivers. In this case, depending on the task at hand and the chosen criterion, efficiency is understood as a reduction of the number of drivers involved in the implementation of the specified standard train schedule, improved uniformity of alternation of driver work and rest periods, as well as reduction of the duration of down time within work shifts.Methods. The study presented in the paper uses the graph theory, recursive and heuristic algorithms.Results. The authors have developed recursive algorithms for preparing work schedules for main metro train drivers and substitute drivers that operate during main drivers’ lunch breaks. The developed algorithms are used in the intelligent system for metro train driver work schedule planning. The algorithm for generating main driver work schedule includes driver allocation to a number of work lines preliminarily defined based on the rolling stock operation schedule for the purpose of ensuring traffic according to the planned metro train schedule. The algorithm for generating substitute driver work schedule involves substitute driver work time planning based on the possibility of arranging lunch breaks for main drivers.Conclusion. The paper presents the results of a trial of the developed intelligent system for train driver work planning for the Moscow Metro using the example of the Zamoskvoretskoye Depot of the Zamoskvoretskaya Line, the Vykhino Depot of the Tagansko-Krasnopresnenskaya Line, as well as the results of the system’s adaptation to the Moscow Central Circle. It also presents a comparative analysis of driver work schedules, i.e., the actual one and one obtained using the developed intelligent system. The application of the developed system may enable as much as a 28% improvement of the efficiency of metro train drivers’ work hour use.
Dependability factors of microprocessor protection software
Authors: Uspensky M. I. 
Published: August 2023

Aim. An analysis of the distinctive features of the functional dependability of software that are common to critical real-time applications in respect to microprocessor protections. Dependability indicators are among the primary operational characteristics of relay protection. As such protections migrate to microprocessor technology, it became necessary to characterise their operation and dependability with software dependability along the hardware dependability. The importance of the tasks it solves in the process of operation puts it into the category of programs used in critical real-time applications. That, in turn, toughens the requirements for their dependability evaluation.Methods. The comparative analysis allowed evaluating the unity of the examined operational dependability factors of the software as part the examined applications, while identifying certain distinctive features that are common to relay protections that use microprocessors.Results. The paper provides an example of such estimation that shows that, given all the complexity of testing microprocessor protection programs, the contribution of software errors still is 2,5% from the overall contribution.Conclusion. It is shown that microprocessor protection programs fall into the category of critical real-time application programs, which allows using the experience and characteristics of such software as part of relay protection. Nevertheless, the paper cites certain distinctive features that are common only to microprocessor-based relay protection. Further tasks are specified.
Comprehensive Analysis of Ligand-receptor Interactions in Colon
Adenocarcinoma to Identify of Tumor Microenvironment Oxidative
Stress and Prognosis Model
Authors: Hu Jun , Zhu Wenbo , Wang Wenpeng , Yue Xin , Zhao Peng , Kong Dalu 
Published: August 2023

Background:
Single-cell technology enables a deep study on the mechanism of cancers.
This work delineated the function of ligand-receptor interaction in colon adenocarcinoma (COAD),
and developed a LR pairs-based prognostic model.

Methods:
For identifying important LR pairs, Single-cell RNA sequencing data of COAD was included.
Unsupervised consensus clustering constructed molecular subtypes. LASSO established a
prognostic model. Infiltration of 22 immune cells was evaluated by Cibersort. Enrichment score of
oxidative stress related pathways was determined by SsGSEA in each patient.

Results:
Forty-seven LR pairs were closely associated with the prognosis of COAD. Three molecular
subtypes were differentiated according to 47 LR pairs, which displayed differential clinical features
and molecular features. There were significant differences in immune T cell lytic activity
among different subtypes. In clust1 with poor prognosis, significantly enriched oncogenic pathways
were found, especially epithelial-mesenchymal transition (EMT). Additionally, it has been
found that clust3 had significantly higher immune infiltration. A prognostic model containing eight
LR pairs (PDGFB-PDGFRA, FLT4-VEGFC, CSF1R-CSF1, DLL1-NOTCH4, PDGFB-LRP1, DLL1-
NOTCH3, FLT4-PDGFC, and NRP2-PGF) was established, which could effectively divide
samples into low-risk and high-risk groups. Significantly higher oxidative stress was found among
high-risk patients.

Conclusion:
This study integrated expression data and single-cell data for demonstrating the effectiveness
of LR pairs in establishing the prognostic model and constructing molecular subtypes.
Prognostic LR pairs may contribute to tumorigenesis and progression in COAD. The prognostic
model was the potential for predicting prognosis and guiding immunotherapy for COAD patients.
MiR-301b-3p can be used as a Potential Marker for the Diagnosis of Lung
Adenocarcinoma
Authors: Qi Weibo , Niu Niu , Zhao Junjie , Liu Haitao , Yang Fan 
Published: August 2023

Background:
The involvement of aberrantly expressed miR-301b-3p has been discovered
in diverse human tumors. Our study was primarily centered around the role of miR-301b-3p in
diagnosing lung adenocarcinoma (LUAD).

Method:
We used the TCGA database to download the TCGA-LUAD dataset and selected miR-
301b-3p as the object of our study by differential expression analysis of miRNAs combined with
previous studies. The LUAD diagnostic model was constructed utilizing machine learning based
on miR-301b-3p expression. The predictive performance of the diagnostic model was found to be
excellent by ROC curves combined with the clinical information of the dataset samples. GSEA,
GO, and KEGG enrichment analyses demonstrated that miR-301b-3p may mediate the cell cycle
by regulating the expression of hormones. Subsequently, combined with tumor immunity and mutation
analysis, it was found that patients in the low-expression group had better immune infiltration,
indicating that their response rate to immunotherapy may be relatively high. Finally, a mouse
xenograft model was constructed to verify how miR-301b-3p affected LUAD progression in mice.

Result:
The results illustrated that overexpressed miR-301b-3p could cause faster tumor growth in
mice. On the contrary, the growth of LUAD could be impeded by the downregulated miR-301b-3p
expression. It was suggested that miR-301b-3p had a crucial part in LUAD progression.

Conclusion:
Overall, the diagnostic performance of the LUAD diagnostic model constructed based
on miR-301b-3p is great, and the model can be used as a potential diagnostic marker for LUAD to
provide new ideas for clinical diagnosis.
Recent Advances in Biomedical Nanotechnology Related to Natural
Products
Authors: Xia Qing , Liang Tingting , Zhou Yue , Liu Jun , Tang Yue , Liu Feila 
Published: August 2023

Abstract:
Natural product processing via nanotechnology has opened the door to innovative and
significant applications in medical fields. On one hand, plants-derived bioactive ingredients such
as phenols, pentacyclic triterpenes and flavonoids exhibit significant pharmacological activities,
on another hand, most of them are hydrophobic in nature, posing challenges to their use. To overcome
this issue, nanoencapsulation technology is employed to encapsulate these lipophilic compounds
and enhance their bioavailability. In this regard, various nano-sized vehicles, including
degradable functional polymer organic compounds, mesoporous silicon or carbon materials, offer
superior stability and retention for bioactive ingredients against decomposition and loss during
delivery as well as sustained release. On the other hand, some naturally occurring polymers, lipids
and even microorganisms, which constitute a significant portion of Earth's biomass, show promising
potential for biomedical applications as well. Through nano-processing, these natural products
can be developed into nano-delivery systems with desirable characteristics for encapsulation a
wide range of bioactive components and therapeutic agents, facilitating in vivo drug transport.
Beyond the presentation of the most recent nanoencapsulation and nano-processing advancements
with formulations mainly based on natural products, this review emphasizes the importance of
their physicochemical properties at the nanoscale and their potential in disease therapy.
Thrombin - A Molecular Dynamics Perspective
Authors: Wu Dizhou , Prem Athul , Xiao Jiajie , Salsbury Freddie R. 
Published: August 2023

Abstract:
Thrombin is a crucial enzyme involved in blood coagulation, essential for maintaining circulatory
system integrity and preventing excessive bleeding. However, thrombin is also implicated in
pathological conditions such as thrombosis and cancer. Despite the application of various experimental
techniques, including X-ray crystallography, NMR spectroscopy, and HDXMS, none of these
methods can precisely detect thrombin's dynamics and conformational ensembles at high spatial and
temporal resolution. Fortunately, molecular dynamics (MD) simulation, a computational technique
that allows the investigation of molecular functions and dynamics in atomic detail, can be used to explore
thrombin behavior. This review summarizes recent MD simulation studies on thrombin and its
interactions with other biomolecules. Specifically, the 17 studies discussed here provide insights into
thrombin's switch between 'slow' and 'fast' forms, active and inactive forms, the role of Na+ binding,
the effects of light chain mutation, and thrombin's interactions with other biomolecules. The findings
of these studies have significant implications for developing new therapies for thrombosis and cancer.
By understanding thrombin's complex behavior, researchers can design more effective drugs and
treatments that target thrombin.
Development and Validation of In-vitro Release Study of Molnupiravir
Capsules by RP-HPLC
Authors: Singhal Shipra , Sharma Aman , Malik Anirudh , Dahiya Meenakshi , Singh Jadaun Gaurav Pratap , Patil Vaishali M. , Kaushik Shikha 
Published: August 2023

Introduction:
The coronavirus disease-2019 (COVID-19) outbreak all over the world has
led researchers to strive to develop treatment and preventive measures to control its progression.

Methods:
Molnupiravir, a prodrug of the synthetic nucleoside derivative N-4-hydroxycytidine
was found to be a promising candidate against Severe Acute Respiratory Syndrome Coronavirus
2 (SARS-CoV-2).

Results:
It could significantly reduce the risk of hospitalization and mortality among patients with
positive SARS-CoV-2 reports. In this study, an RP-HPLC method with UV detection was developed
to determine its dissolution and release in the capsule dosage form. The developed method
was validated as per International Council for Harmonization (ICH) guidelines.

Conclusion:
The method was evaluated and validated for its applicability using various parameters.
It was found to be a simple, rapid, selective, sensitive, accurate, precise, robust and rugged
method.