bluuebunny/crossref_metadata_2025_split huggingface dataset:
OʻQITUVCHINING KASBIY KRYEATIVLIGI VA UNI RIVOJLANTIRISHNING ILMIY- NAZARIY ASOSLARI
Authors:   Samarqand davlat universiteti, T Jumaniyozova M 
Published: March 2023

Maqolada kreativlik tushunchasi, o‘qituvchining kasbiy kreativligini rivojlantirish

bo‘yicha olib borilgan tadqiqotlar, psixologik-pedagogik manbalar tahlili, kasbiy kreativlikning mezonlari,

ijodiy rivojlanayotgan ta’lim makonini yaratish haqida so‘z yuritiladi.
Yosh avlod ma’naviy-axloqiy fazilatlarini shakllantirishda oila, mahalla va ta’lim muassasasi uzluksizligini ta’minlashning ijtimoiy pedagogik masalalari
Authors:   Samarqand davlat universiteti, Q  O‘tanov O ‘ 
Published: March 2023

Maqolada oila – mahalla – ta’lim uzviyligi asosida yoshlarni oilaga, ijtimoiy

hayotga tayyorlashda turli salbiy oqibatlarning oldini olishda o‘zaro hamkorlik faoliyatlari, ular o‘rtasidagi

o‘zaro manfaatli hamkorlik aloqalari bayon etilgan. Bundan tashqari oila, mahalla, ta’lim muassasasi

hamkorligida amal qilinishi lozim bo‘lgan tamoyillar va hal etilishi lozim bo‘lgan dolzarb muammolar o‘z

aksini topgan.
Colorimetric Detection of Glucose Based on Discrete Chiral Au Nanorods
Authors: Pan Xiaobing , Li Haoyu , Chen Xuan , Wang Shenli , Mourdikoudis Stefanos , Luo Taotao , Wong Kwok‐yin , Zheng Guangchao 
Published: February 2025

AbstractThe fabrication of discrete chiral Au nanorods (c‐Au NRs) used as new emerging chiral plasmonic nanozyme for colorimetric detection of glucose is presented. It is found that the colorimetric detection of glucose based on the c‐Au NRs is dependent on chiral geometries and polarized light. In comparison to Lc‐Au NRs, Dc‐Au NRs possess a lower linear concentration range of glucose detection and limit of detection (LOD) due to their higher affinity capacity. More importantly, polarized light (LP: linearly polarized light; LCP: left‐handed circularly polarized light; RCP: right‐handed circularly polarized light) can enantioselectively and markedly improve the LOD of glucose due to circularly polarized hot electrons and electromagnetic field. For instance, the LOD is lower under the RCP irradiation for Dc‐Au NRs in comparison to that under LCP irradiation. Similarly, the LOD is higher under the RCP irradiation for Lc‐Au NRs than that under LCP irradiation. Our work not only provides a novel strategy for the detection of glucose levels, but also reveals the chiral interaction between chiral matters and chemical substrates, which will be helpful in the field of chiral recognition, chiral catalysis, and chiral drugs.
Compact Device for Monitoring Electret Filter Performance via Electric Field Strength
Authors: Zikulnig Johanna , Lenzhofer Martin , Mayer Christoph , Grabher Günter , Berchtold Tobias , Kosel Jürgen 
Published: February 2025

AbstractThe performance of electret filters, widely used in various air filtration systems, can degrade over time due to environmental factors such as humidity and elevated temperatures. This study introduces a compact electric field sensor designed for quasi‐continuous monitoring of electret filter performance. The sensor measures the electric field strength of the filter, which correlates with its efficiency. Testing under various conditions demonstrates high specificity (0.93), sensitivity (0.8), and precision (0.89) in measuring electric field strength as an indicator of filter efficiency. Statistically significant p‐values (<0.01) confirm the sensor's accuracy. The developed device can be integrated into various filtration systems, including smart masks, air conditioning, hospital ventilation units, and aircraft air purification systems, to ensure sustained performance and improved air quality in critical environments.
A Silk Fibroin Nanoparticle Hydrogel Loaded With NK1R Antagonist Has Synergistic Anti‐Inflammatory and Reparative Effects on Dry Eye Disease
Authors: Gong Bo , Liu Yi , Li Huan , Ju Xueming , Li Dongfeng , Zou Yuhao , Guo Xiaoxin , Dong Kai , Xiao Jialing , Wu Weijia , Chai Renjie , Zhang Ruifan , Yu Man 
Published: February 2025

AbstractDry eye disease (DED) is a multifactorial illness affecting tears and the ocular surface. The neurokinin 1 receptor (NK1R) is a target for controlling T helper 17 (Th17) and regulatory T cell (Treg) imbalances. This work creates a silk fibroin (SF) nanoparticle hydrogel that targets NK1R with CP‐99,994 (CP). Combining CP and SF to generate stable nanoparticles while integrating a flexible hydrogel material results in a sustained‐release ophthalmic drop formulation (SF@CP@Gel), which provides a long‐lasting ocular formulation with anti‐inflammatory and reparative properties. SF@CP@Gel could maintain a stable CP concentration for 25 h with detectable biological activity. The cell counting kit‐8 and 2,7‐DHL‐DA results reveal that SF@CP@Gel has no cytotoxic effect on human corneal epithelial cells (HCECs) and decreases the reactive oxygen species level in oxidatively damaged HCECs. Cell scratch assays demonstrate that SF@CP@Gel can greatly increase HCEC migration and proliferation within 24 h. Furthermore, in vivo therapy with topical SF@CP@Gel twice daily markedly reduce clinical symptoms by reducing the amount of pathogenic Th17 cells while efficiently restoring Treg activity. In summary, this work reveals that SF@CP@Gel might attenuate DED by inhibiting NK1R‐mediated SP signaling and thereby modulating the Th17/Treg ratio, a potential anti‐inflammatory and repair treatment method for DED.
The Antiviral Efficacy and Safety of Azvudine in Hospitalized SARS‐CoV‐2 Infected Patients with Liver Diseases Based on a Multicenter, Retrospective Cohort Study
Authors: Sun Junyi , Yang Mengzhao , Su Guanyue , Wang Ling , Hu Xiaobo , Zhou Yongjian , Cui Guangying , Qian Guowu , Yuan Yiqiang , Hu Xinjun , Li Silin , Luo Hong , Zhang Shixi , Li Guangming , Zhang Donghua , Li Guotao , Cheng Ming , Yu Zujiang , Ren Zhigang 
Published: February 2025

AbstractDespite azvudine being prioritized for the treatment of severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2) infection, its effectiveness and safety remain inadequately substantiated in hospitalized SARS‐CoV‐2 infected patients with liver diseases. A retrospective nine‐center cohort study along with an independent validation cohort is conducted to examine the efficacy of azvudine (Clinical Trial Registration Number: NCT06349655). The primary outcome is all‐cause mortality and the secondary outcome is composite disease progression. Efficacy is assessed via Kaplan–Meier analysis and Cox regression, with subgroup and sensitivity analyses for further validation. Among 32 864 hospitalized SARS‐CoV‐2 infected patients, 1022 eligible azvudine recipients, and 1022 controls are included through propensity score match. Kaplan–Meier analysis reveals that azvudine treatment is associated with a lower risk of all‐cause mortality and composite disease progression (both p<0.0001). Cox regression analysis suggests azvudine recipients could have a 39% lower risk of all‐cause mortality than controls (95% confidence interval [CI]: 0.468–0.795, p<0.001), but with no notable significance in composite disease progression (hazard ratio: 0.85, 95% CI: 0.686‐1.061, p = 0.154). Subgroup analysis suggests that azvudine has a greater benefit for both all‐cause mortality and composite disease progression in patients with kidney diseases or without autoimmune diseases. Three sensitivity analyses and validation cohorts confirm the robustness of the findings. Safety analysis observes few adverse events in azvudine recipients. Within 15 days after azvudine administration, no significant difference in liver function indexes and kidney function indexes is observed between the two groups except for a few time points. These findings demonstrate that azvudine shows potential clinical efficacy in improving all‐cause mortality in hospitalized SARS‐CoV‐2 infected patients with liver diseases, with acceptable adverse effects.
UFMylation of NLRP3 Prevents Its Autophagic Degradation and Facilitates Inflammasome Activation
Authors: Jing Jiongjie , Yang Fan , Wang Ke , Cui Mintian , Kong Ni , Wang Shixi , Qiao Xiaoyue , Kong Fanyu , Zhao Dongyang , Ji Jinlu , Tang Lunxian , Gao Jiaxin , Cong Yu‐Sheng , Ding Deqiang , Chen Kun 
Published: February 2025

AbstractNLRP3 (NOD, LRR and pyrin domain‐containing protein 3) inflammasome is important for host defense against infections and maintaining homeostasis. Aberrant activation of NLRP3 inflammasome is closely related to various inflammatory diseases. Post‐translational modifications are critical for NLRP3 inflammasome regulation. However, the mechanism of NLRP3 inflammasome activation remains incompletely understood. Here, it is demonstrated that the Ufm1 E3 ligase Ufl1 mediated UFMylation is essential for NLRP3 inflammasome activation. Mechanistically, Ufl1 binds and UFMylates NLRP3 in the priming stage of NLRP3 activation, thereby sustaining the stability of NLRP3 by preventing NLRP3 K63‐linked ubiquitination and the subsequent autophagic degradation. It is further demonstrated that myeloid cell‐specific Ufl1 or Ufm1 deficiency in mice significantly alleviated inflammatory responses and tissue damage following lipopolysaccharide (LPS)‐induced endotoxemia and alum‐induced peritonitis. Thus, the findings offer new insights into potential therapeutic targets for NLRP3 inflammasome‐related diseases by targeting the UFMylation system.
GABP Promotes Mesangial Cell Proliferation and Renal Fibrosis Through GLI1 in Diabetic Nephropathy
Authors: Du Lei , Liu Sijie , Lu Yinfei , Ren Dongxue , Yu Xiujuan , Hu Yue , Yang Tingting , Yang Qun , Ming Jingxian , Zhang Jiawei , Yin Xiaoxing , Lu Qian 
Published: February 2025

AbstractAbnormal proliferation of mesangial cells is a hallmark of diabetic nephropathy (DN). However, the cellular signaling mechanisms that regulate this proliferation remain poorly understood. In this study, it is demonstrated that GA‐binding protein (GABP), a member of the ETS family of transcription factors composed of GABPα and GABPβ, plays a significant role in the development of renal fibrosis by modulating mesangial cell proliferation. Notably, the deficiency of GABP in mesangial cells inhibits hyperglycemia‐induced proliferation and mitigates renal fibrosis in a murine model of type 2 diabetes mellitus (T2DM). RNA sequencing analysis identifies GLI Family Zinc Finger 1 (GLI1) as the principal downstream effector of GABP in diabetic mice, serving as a crucial regulator of the G1/S transition within the cell cycle. Subsequent investigations have demonstrated that GABP interacts with the GLI1 promoter, facilitating mesangial cell proliferation via GLI1‐dependent pathways. This is evidenced by the fact that GLI1 knockdown abrogates the proliferation of mesangial cells with GABP overexpression. Consequently, GABP emerges as a pivotal regulator of renal fibrosis and represents a promising therapeutic target for the treatment of diabetic nephropathy.
Acoustic Rayleigh Wave Turbulence in Soft Viscoelastic Matter
Authors: Kharbedia Mikheil , López‐Menéndez Horacio , García Basilio Javier , Velarde Manuel G. , Monroy Francisco 
Published: February 2025

AbstractEvidence of discrete acoustic Rayleigh wave turbulence (DARWT) is reported on the free surface of complex viscoelastic materials under monochromatic excitation. These surface elastic non‐linear Rayleigh waves exhibit dispersionless dynamics governed by bulk shear rigidity at coexistence with dispersive capillary waves supported by surface tension. Using Laser Doppler Vibrometry (LDV), the discrete Kolmogorov–Zakharov (KZ) spectrum is measured, characterized by a power‐law envelope in an inertial cascade of discrete harmonics. When shear rigidity dominates, KZ‐spectrum characterized by an ω−5/2 acoustic fingerprint is observed as a conservative solid‐like scaling, theoretically predicted from the exact three‐wave dispersionless resonance. Conversely, when surface tension dominates over bulk stresses, dispersive CW behavior under the usual ω−17/6 liquid‐like scaling is recovered. These findings support a unique theoretical framework for understanding conservative DARWT and advance the field of surface wave turbulence in viscoelastic media with markedly non‐Newtonian rheology. Acoustic Rayleigh wave turbulence represents a conservative mechanism for dispersionless energy transport through weakly nonlinear wave interactions across scales, with relevance to various physics fields, from soft matter and biological systems up to geophysical flows.
Chchd10: A Novel Metabolic Sensor Modulating Adipose Tissue Homeostasis
Authors: Wu Xiaoping , Zhang Zixuan , Li Jingjing , Zong Jiuyu , Yuan Lufengzi , Shu Lingling , Cheong Lai Yee , Huang Xiaowen , Jiang Mengxue , Ping Zhihui , Xu Aimin , Hoo Ruby L.C. 
Published: February 2025

AbstractDysregulation of adipose tissue (AT) homeostasis in obesity contributes to metabolic stress and disorders. Here, we identified that Coiled‐coil‐helix‐coiled‐coil‐helix domain containing 10 (Chchd10) is a novel regulator of AT remodeling upon excess energy intake. Chchd10 is significantly reduced in the white adipose tissue (WAT) of mice in response to high‐fat diet (HFD) feeding. AT‐Chchd10 deficiency accelerates adipogenesis predominantly in subcutaneous AT of mice to store excess energy in response to short‐term HFD feeding while upregulates glutathione S‐transferase A4 (GSTA4) to facilitate 4‐HNE clearance mainly in visceral AT to prevent protein carbonylation‐induced cell dysfunction after long‐term HFD feeding. Hence, Chchd10 deficiency attenuates diet‐induced obesity and related metabolic disorders in mice. Mechanistically, Chchd10 deficiency enhances adipogenesis and GSTA4 expression by activating TDP43/Raptor/p62/Keap1/NRF2 axis. Notably, the beneficial effect of Chchd10 deficiency is eliminated in hypertrophic adipocytes, where p62 is strikingly reduced. Collectively, Chchd10 is a metabolic sensor maintaining AT homeostasis, and the loss of p62 in adipose tissue under obese conditions impairs Chchd10‐mediated AT remodeling.
3‐D Sustained‐Release Culture Carrier Alleviates Rat Intervertebral Disc Degeneration by Targeting STING in Transplanted Skeletal Stem Cells
Authors: Luo Liwen , Zhang Shiyu , Gong Junfeng , Zhang Ji , Xie Peng , Yin Jun , Zhang MengJie , Zhang Cong , Chen Hong , Liu Yao , Ni Bing , Li Changqing , Tian Zhiqiang 
Published: February 2025

AbstractThe hypoxic and high‐pressure microenvironment of the intervertebral discs poses a major challenge to the survival and therapeutic efficiency of exogenous stem cells. Therefore, improving the utilization efficiency and therapeutic effect of exogenous stem cells to delay intervertebral disc degeneration (IVDD) is of great importance. Here, hypoxic induction studies are conducted in vivo and in vitro using rat costal cartilage‐derived skeletal stem cells (SSCs) and find that hypoxia activates the cyclic guanosine monophosphate–adenosine monophosphate synthase (cGAS)/stimulator of interferon genes (STING) signaling pathway and increased reactive oxygen species (ROS) accumulation, triggering ferroptosis in SSCs through hypoxia‐inducible factor‐1 alpha‐dependent mitophagy. Progressive hypoxia preconditioning reduce STING expression and ROS accumulation, inducing SSCs differentiation into nucleus pulposus‐like cells via the Wnt signaling pathway. Considering this, a 3‐D sustained‐release culture carrier is generated by mixing SSCs with methacrylated hyaluronic acid and polydopamine nanoparticles coated with the STING inhibitor C‐176 and evaluated its inhibitory effect on IVDD. This carrier is demonstrated to inhibit the cGAS/STING pathway and prevent ROS accumulation by continuously releasing C‐176‐coated polydopamine nanoparticles, thereby reducing ferroptosis, promoting differentiation, and ultimately attenuating IVDD, suggesting its potential as a novel treatment strategy.
An Anti‐CD147 Antibody−Drug Conjugate Mehozumab‐DM1 is Efficacious Against Hepatocellular Carcinoma in Cynomolgus Monkey
Authors: Huang Wan , Zhong Liping , Shi Ying , Ma Qingzhi , Yang Xiangmin , Zhang Hongmei , Zhang Jing , Wang Ling , Wang Kun , Li Jingzhuo , Zou Jie , Yang Xu , Yang Liu , Zeng Qingmei , Jing Lin , Chen Zhi‐Nan , Zhao Yongxiang 
Published: February 2025

AbstractEffective treatment strategies are urgently needed for hepatocellular carcinoma (HCC) patients due to frequent therapeutic resistance and recurrence. Antibody‐drug conjugate (ADC) is a specific antibody‐drug conjugated with small molecular compounds, which has potent killing activity against cancer cells. However, few ADC candidates for HCC are undergoing clinical evaluation. CD147 is a tumor‐associated antigen that is highly expressed on the surface of tumor cells. Here CD147 is found significantly upregulated in tumor tissues of HCC. Mehozumab‐DM1, a humanized anti‐CD147 monoclonal antibody conjugated with Mertansine (DM1) is developed. Mehozumab‐DM1 is effectively internalized by cancer cells and demonstrated potent antitumor efficacy in HCC cells. In vivo evaluation of Mehozumab‐DM1 is conducted in a CRISPR‐mediated PTEN and TP53 mutation cynomolgus monkey liver cancer model, which is poorly responsive to sorafenib treatment. Mehozumab‐DM1 demonstrated potent tumor inhibitory efficacy at doses of 0.2 and 1.0 mg kg−1 treatment groups in cynomolgus monkey. No treatment‐related adverse reactions or body weight loss are observed. Interestingly, Mehozumab‐DM1 treatment induced RIPK‐dependent tumor cell necroptosis through inhibiting IκB kinase/NF‐κB pathway. In conclusion, Mehozumab‐DM1 potently inhibits hepatoma through effective internalization to release payload and inducing cell necroptosis to enhance the bystander effect, which is a promising treatment for refractory HCC.
Pepticombisomes: Biomimetic Vesicles Crafted From Recombinant Supercharged Polypeptides with Uniformly Distributed Side‐Chains
Authors: Söder Dominik , Schadt Melina , Petrovskii Vladislav S. , Haraszti Tamás , Rahimi Khosrow , Potemkin Igor I. , Kostina Nina Yu. , Rodriguez‐Emmenegger Cesar , Herrmann Andreas 
Published: February 2025

AbstractCell membranes play a key role in bottom‐up synthetic biology, as they enable interaction control, transport, and other essential functions. These ultra‐thin, flexible, yet stable structures form through the self‐assembly of lipids and proteins. While liposomes are common mimics, their synthetic membranes often fail to replicate natural properties due to poor structural control. To address this, pepticombs are introduced, a new family of supramolecular building blocks. They are synthesized by regularly appending anionic surfactants with lipid‐long alkyl tails to cationic amino acid residues of recombinant elastin‐like supercharged unfolded polypeptides (SUPs). Using microscopy techniques and molecular dynamics simulations, the formation of giant unilamellar vesicles, termed pepticombisomes, is demonstrated and their membrane properties are characterized. The molecular topology of pepticombs allows for precise mimicry of membrane thickness and flexibility, beyond classic polymersomes. Unlike the previously introduced ionically‐linked comb polymers, all pepticombs exhibit a uniform degree of polymerization, composition, sequence, and spontaneous curvature. This uniformity ensures consistent hydrophobic tail distribution, facilitating intermolecular hydrogen bonding within the backbone. This generates elastic heterogeneities and the concomitant formation of non‐icosahedral faceted vesicles, as previously predicted. Additionally, pepticombisomes can incorporate functional lipids, enhancing design flexibility.
CpG‐Based Nanovaccines Enhance Ovarian Cancer Immune Response by Gbp2‐Mediated Remodeling of Tumor‐Associated Macrophages
Authors: Xiong Jiaqiang , Huang Juyuan , Xu Hanxiao , Wu Qiuji , Zhao Jiahui , Chen Yurou , Fan Guanlan , Guan Haotong , Xiao Rourou , He Zhaojin , Wu Siqi , Ouyang Wenliang , Wang Shixuan , Zhang Lu , Xia Peng , Zhang Wei , Wu Meng 
Published: February 2025

AbstractCpG oligodeoxynucleotides (CpG), as an immunoadjuvant, can facilitate the transformation of tumor‐associated macrophages (TAMs)into tumoricidal M1 macrophages. However, the accumulation of free CpG in tumor tissues remains a substantial challenge. To address this, a nanovaccine (PLGA‐CpG@ID8‐M) is engineered by encapsulating CpG within PLGA using ID8 ovarian cancer cell membranes (ID8‐M). This nanovaccine demonstrates remarkable efficacy in reprogramming TAMs in ovarian cancer and significantly extends survival in ID8‐bearing mice. Notably, these findings indicate that the nanovaccine can also mitigate chemotherapy‐induced immunosuppression by increasing the proportion of M1‐like TAMs and reducing the expression of CD47 on tumor cells, thereby achieving a synergistic effect in tumor immunotherapy. Mechanistically, through transcriptome sequencing (RNA‐seq), single‐cell RNA sequencing (scRNA‐seq), and mass spectrometry‐based proteomics, it is elucidated that the nanovaccine enhances the expression of Gbp2 and promotes the recruitment of Pin1, which activates the NFκB signaling pathway, leading to the M1 polarization of TAMs. Furthermore, macrophages with elevated Gbp2 expression significantly inhibit tumor growth in both ID8 ovarian cancer and 4T1 breast cancer models. Conversely, targeting Gbp2 diminishes the antitumor efficacy of the nanovaccine in vivo. This study offers an innovative approach to immunotherapy and elucidates a novel mechanism (Gbp2‐Pin1‐NFκB pathway) for remodeling TAMs.
Hybrid EMT Phenotype and Cell Membrane Tension Promote Colorectal Cancer Resistance to Ferroptosis
Authors: Wei Xiaowei , Ge Yutong , Zheng Yaolin , Zhao Sunyan , Zhou Yuhan , Chang Yuhan , Wang Nuofan , Wang Xiumei , Zhang Juan , Zhang Xuanchang , Hu Liqiao , Tan Youhua , Jia Qiong 
Published: February 2025

AbstractIntratumoral heterogeneity, including epithelial–mesenchymal transition (EMT), is one major cause of therapeutic resistance. The induction of ferroptosis, an iron‐dependent death, has the potential in overcoming this resistance to traditional treatment modalities. However, the roles of distinct EMT phenotypes in ferroptosis remain an enigma. This study reports that 3D soft fibrin microenvironment confers colorectal cancer (CRC) cells hybrid EMT phenotype and high level of resistance to ferroptosis. The activation of histone acetylation and WNT/β‐catenin signaling drives this EMT phenotypic transition, which promotes the defense of 3D CRCs against ferroptosis via glutathione peroxidases/ferritin signaling axis. Unexpectedly, E‐cadherin knockout in 3D but not 2D CRCs mediates an integrin β3 marked‐late hybrid EMT state and further enhances the resistance to ferroptosis via integrin‐mediated tension and mitochondrial reprogramming. The inhibition of integrin αvβ3‐mediated tension and WNT/β‐catenin‐mediated hybrid EMT sensitizes 3D CRCs with and without E‐cadherin deficiency to ferroptosis in vivo, respectively. Further, the EMT phenotype of patient‐derived tumoroids is associated with CRC therapeutic resistance. In summary, this study uncovers previously unappreciated roles of hybrid EMT and cell membrane tension in ferroptosis, which not only predict the treatment efficacy but also potentiate the development of new ferroptosis‐based targeted therapeutic strategies.