bluuebunny/crossref_metadata_2025_split huggingface dataset:
Knockdown of Porf-2 restores visual function after optic nerve crush injury
Authors: Chen Di , Sun Yi-Yu , Zhou Lai-Yang , Han Xu , Yang Shuo , Hong Fei-Yang , Yuan Yuan , Wu Xiao-Hua , Huang Guo-Hui , Cheng Yuan-Chi , Huang Ju , Feng Dong-Fu 
Published: August 2023

AbstractRetinal ganglion cells (RGCs), the sole output neurons in the eyes, are vulnerable to diverse insults in many pathological conditions, which can lead to permanent vision dysfunction. However, the molecular and cellular mechanisms that contribute to protecting RGCs and their axons from injuries are not completely known. Here, we identify that Porf-2, a member of the Rho GTPase activating protein gene group, is upregulated in RGCs after optic nerve crush. Knockdown of Porf-2 protects RGCs from apoptosis and promotes long-distance optic nerve regeneration after crush injury in both young and aged mice in vivo. In vitro, we find that inhibition of Porf-2 induces axon growth and growth cone formation in retinal explants. Inhibition of Porf-2 provides long-term and post-injury protection to RGCs and eventually promotes the recovery of visual function after crush injury in mice. These findings reveal a neuroprotective impact of the inhibition of Porf-2 on RGC survival and axon regeneration after optic nerve injury, providing a potential therapeutic strategy for vision restoration in patients with traumatic optic neuropathy.
SOSSB1 and SOSSB2 mutually regulate protein stability through competitive binding of SOSSA
Authors: Zhang Qi , Hao Rongjiao , Chen Hongxia , Zhou Gangqiao 
Published: August 2023

AbstractHuman single-stranded DNA-binding protein homologs hSSB1 (SOSSB1) and hSSB2 (SOSSB2) make a vital impact on maintaining genome stability as the B subunits of the sensor of single-stranded DNA complex (SOSS). However, whether and how SOSSB1 and SOSSB2 modulate mutual expression is unclear. This study, demonstrated that the depletion of SOSSB1 in cells enhances the stability of the SOSSB2 protein, and conversely, SOSSB2 depletion enhances the stability of the SOSSB1 protein. The levels of SOSSB1 and SOSSB2 proteins are mutually regulated through their competitive binding with SOSSA which associates with the highly conservative OB-fold domain in SOSSB1 and SOSSB2. The destabilized SOSSB1 and SOSSB2 proteins can be degraded via the proteasome pathway. Additionally, the simultaneous loss of SOSSB1 and SOSSB2 aggravates homologous recombination (HR)-mediated DNA repair defects, enhances cellular radiosensitivity and promotes cell apoptosis. In conclusion, in this study, we showed that SOSSB1 and SOSSB2 positively regulate HR repair and the interaction between SOSSA and SOSSB1 or SOSSB2 prevents the degradation of SOSSB1 and SOSSB2 proteins via the proteasome pathway.
Chemoenzymatic synthesis of genetically-encoded multivalent liquid N-glycan arrays
Authors: Lin Chih-Lan , Sojitra Mirat , Carpenter Eric J. , Hayhoe Ellen S. , Sarkar Susmita , Volker Elizabeth A. , Wang Chao , Bui Duong T. , Yang Loretta , Klassen John S. , Wu Peng , Macauley Matthew S. , Lowary Todd L. , Derda Ratmir 
Published: August 2023

AbstractCellular glycosylation is characterized by chemical complexity and heterogeneity, which is challenging to reproduce synthetically. Here we show chemoenzymatic synthesis on phage to produce a genetically-encoded liquid glycan array (LiGA) of complex type N-glycans. Implementing the approach involved by ligating an azide-containing sialylglycosyl-asparagine to phage functionalized with 50–1000 copies of dibenzocyclooctyne. The resulting intermediate can be trimmed by glycosidases and extended by glycosyltransferases yielding a phage library with different N-glycans. Post-reaction analysis by MALDI-TOF MS allows rigorous characterization of N-glycan structure and mean density, which are both encoded in the phage DNA. Use of this LiGA with fifteen glycan-binding proteins, including CD22 or DC-SIGN on cells, reveals optimal structure/density combinations for recognition. Injection of the LiGA into mice identifies glycoconjugates with structures and avidity necessary for enrichment in specific organs. This work provides a quantitative evaluation of the interaction of complex N-glycans with GBPs in vitro and in vivo.
Engaging an HIV vaccine target through the acquisition of low B cell affinity
Authors: Ronsard Larance , Yousif Ashraf S. , Nait Mohamed Faez Amokrane , Feldman Jared , Okonkwo Vintus , McCarthy Caitlin , Schnabel Julia , Caradonna Timothy , Barnes Ralston M. , Rohrer Daniel , Lonberg Nils , Schmidt Aaron , Lingwood Daniel 
Published: August 2023

AbstractLow affinity is common for germline B cell receptors (BCR) seeding development of broadly neutralizing antibodies (bnAbs) that engage hypervariable viruses, including HIV. Antibody affinity selection is also non-homogenizing, insuring the survival of low affinity B cell clones. To explore whether this provides a natural window for expanding human B cell lineages against conserved vaccine targets, we deploy transgenic mice mimicking human antibody diversity and somatic hypermutation (SHM) and immunize with simple monomeric HIV glycoprotein envelope immunogens. We report an immunization regimen that focuses B cell memory upon the conserved CD4 binding site (CD4bs) through both conventional affinity maturation and reproducible expansion of low affinity BCR clones with public patterns in SHM. In the latter instance, SHM facilitates target acquisition by decreasing binding strength. This suggests that permissive B cell selection enables the discovery of antibody epitopes, in this case an HIV bnAb site.
Activating dynamic atomic-configuration for single-site electrocatalyst in electrochemical CO2 reduction
Authors: Hsu Chia-Shuo , Wang Jiali , Chu You-Chiuan , Chen Jui-Hsien , Chien Chia-Ying , Lin Kuo-Hsin , Tsai Li Duan , Chen Hsiao-Chien , Liao Yen-Fa , Hiraoka Nozomu , Cheng Yuan-Chung , Chen Hao Ming 
Published: August 2023

AbstractOne challenge for realizing high-efficiency electrocatalysts for CO2 electroreduction is lacking in comprehensive understanding of potential-driven chemical state and dynamic atomic-configuration evolutions. Herein, by using a complementary combination of in situ/operando methods and employing copper single-atom electrocatalyst as a model system, we provide evidence on how the complex interplay among dynamic atomic-configuration, chemical state change and surface coulombic charging determines the resulting product profiles. We further demonstrate an informative indicator of atomic surface charge (φe) for evaluating the CO2RR performance, and validate potential-driven dynamic low-coordinated Cu centers for performing significantly high selectivity and activity toward CO product over the well-known four N-coordinated counterparts. It indicates that the structural reconstruction only involved the dynamic breaking of Cu–N bond is partially reversible, whereas Cu–Cu bond formation is clearly irreversible. For all single-atom electrocatalysts (Cu, Fe and Co), the φe value for efficient CO production has been revealed closely correlated with the configuration transformation to generate dynamic low-coordinated configuration. A universal explication can be concluded that the dynamic low-coordinated configuration is the active form to efficiently catalyze CO2-to-CO conversion.
Dual donor-acceptor covalent organic frameworks for hydrogen peroxide photosynthesis
Authors: Qin Chencheng , Wu Xiaodong , Tang Lin , Chen Xiaohong , Li Miao , Mou Yi , Su Bo , Wang Sibo , Feng Chengyang , Liu Jiawei , Yuan Xingzhong , Zhao Yanli , Wang Hou 
Published: August 2023

AbstractConstructing photocatalytically active and stable covalent organic frameworks containing both oxidative and reductive reaction centers remain a challenge. In this study, benzotrithiophene-based covalent organic frameworks with spatially separated redox centers are rationally designed for the photocatalytic production of hydrogen peroxide from water and oxygen without sacrificial agents. The triazine-containing framework demonstrates high selectivity for H2O2photogeneration, with a yield rate of 2111 μM h−1(21.11 μmol h−1and 1407 μmol g−1h−1) and a solar-to-chemical conversion efficiency of 0.296%. Codirectional charge transfer and large energetic differences between linkages and linkers are verified in the double donor-acceptor structures of periodic frameworks. The active sites are mainly concentrated on the electron-acceptor fragments near the imine bond, which regulate the electron distribution of adjacent carbon atoms to optimally reduce the Gibbs free energy of O2* and OOH* intermediates during the formation of H2O2.
Environmental gradients reveal stress hubs pre-dating plant terrestrialization
Authors: Dadras Armin , Fürst-Jansen Janine M. R. , Darienko Tatyana , Krone Denis , Scholz Patricia , Sun Siqi , Herrfurth Cornelia , Rieseberg Tim P. , Irisarri Iker , Steinkamp Rasmus , Hansen Maike , Buschmann Henrik , Valerius Oliver , Braus Gerhard H. , Hoecker Ute , Feussner Ivo , Mutwil Marek , Ischebeck Till , de Vries Sophie , Lorenz Maike , de Vries Jan 
Published: August 2023

AbstractPlant terrestrialization brought forth the land plants (embryophytes). Embryophytes account for most of the biomass on land and evolved from streptophyte algae in a singular event. Recent advances have unravelled the first full genomes of the closest algal relatives of land plants; among the first such species was Mesotaenium endlicherianum. Here we used fine-combed RNA sequencing in tandem with a photophysiological assessment on Mesotaenium exposed to a continuous range of temperature and light cues. Our data establish a grid of 42 different conditions, resulting in 128 transcriptomes and ~1.5 Tbp (~9.9 billion reads) of data to study the combinatory effects of stress response using clustering along gradients. Mesotaenium shares with land plants major hubs in genetic networks underpinning stress response and acclimation. Our data suggest that lipid droplet formation and plastid and cell wall-derived signals have denominated molecular programmes since more than 600 million years of streptophyte evolution—before plants made their first steps on land.
Effects of racket moment of inertia on racket head speed, impact location and shuttlecock speed during the badminton smash
Authors: Towler H. , Mitchell S. R. , King M. A. 
Published: August 2023

AbstractHow the racket properties impact performance of the badminton smash is relatively unknown, and further insight could help players/coaches select the most appropriate racket. Three-dimensional position data of the racket and shuttlecock were collected (500 Hz) for 20 experienced badminton players performing a series of forehand smashes with five swingweight ($${I}_{s}$$

I
s

) perturbed rackets, ranging from 85–106 kg·cm2. $${I}_{s}$$

I
s

was calculated using a balance board and simple pendulum method, and modal analysis was performed using laser vibrometry to capture the fundamental frequency and distal node location for each racket. As $${I}_{s}$$

I
s

increased a reduction in racket head speed was found with on average a 0.7 m·s−1 decrease per 5 kg·cm2 increase in $${I}_{s}$$

I
s

, however this did not lead to slower shuttlecock speeds. The impact location tended to move closer to the tip as the fundamental frequency node moved closer to the tip (as $${I}_{s}$$

I
s

increased), providing some evidence that participants may subconsciously strike the shuttlecock at the node location to provide desirable sensory feedback. The increase in racket head speed but not shuttlecock speed was likely due to the distal increase in longitudinal impact location as $${I}_{s}$$

I
s

increased, as well as an increase in effective mass for a given impact location. Additionally, removal of the deformation component (additional racket head speed due to the racket noticeably bending and recovering) of racket head speed increased the effect size of the relationship with $${I}_{s}$$

I
s

, where rackets with greater $${I}_{s}$$

I
s

had larger deformation velocities. The research provides further insight into the smash performance characteristics of experienced badminton players, particularly based on racket properties. Further research is required to confirm the coincidence between node location and longitudinal impact location.
Lightweight and efficient dual-path fusion network for iris segmentation
Authors: Lei Songze , Shan Aokui , Liu Bo , Zhao Yanxiao , Xiang Wei 
Published: August 2023

AbstractIn order to tackle limitations of current iris segmentation methods based on deep learning, such as an enormous amount of parameters, intensive computation and excessive storage space, a lightweight and efficient iris segmentation network is proposed in this article. Based on the classical semantic segmentation network U-net, the proposed approach designs a dual-path fusion network model to integrate deep semantic information and rich shallow context information at multiple levels. Our model uses the depth-wise separable convolution for feature extraction and introduces a novel attention mechanism, which strengthens the capability of extracting significant features as well as the segmentation capability of the network. Experiments on four public datasets reveal that the proposed approach can raise the MIoU and F1 scores by 15% and 9% on average compared with traditional methods, respectively, and 1.5% and 2.5% on average compared with the classical semantic segmentation method U-net and other relevant methods. Compared with the U-net, the proposed approach reduces about 80%, 90% and 99% in terms of computation, parameters and storage, respectively, and the average run time up to 0.02 s. Our approach not only exhibits a good performance, but also is simpler in terms of computation, parameters and storage compared with existing classical semantic segmentation methods.
Orosomucoid 1 promotes colorectal cancer progression and liver metastasis by affecting PI3K/AKT pathway and inducing macrophage M2 polarization
Authors: Yue Lei , Xu Xiaozhang , Dai Shipeng , Xu Fan , Zhao Wenhu , Gu Jian , Dai Xinzheng , Qian Xiaofeng 
Published: August 2023

AbstractApproximately 25–30% of those affected by colorectal cancer (CRC), the most prevalent gastrointestinal malignancy, develop metastases. The survival rate of patients with liver metastasis of CRC (CRLM) remains low owing to its unpredictability and a lack of biomarkers that can be applied to distinguish groups at higher risk for CRLM among patients with CRC. Therefore, our study aimed to find biomarkers that can predict the risk of CRLM. Screening of the Gene Expression Omnibus database, supported by an analysis of clinically obtained tissue and serum data using qPCR and ELISA, in an attempt to identify relevant biomarkers, enabled us to determine that orosomucoid 1 (ORM1) was differentially expressed in liver metastases and primary tumors of patients with CRC. Functionally, overexpression of ORM1 promoted the epithelial-mesenchymal transition and the proliferative, migratory, and invasive activities of MC38 cells and activated the PI3K/AKT signaling pathway. Moreover, MC38 cells overexpressing ORM1 enhanced the tumor immune microenvironment by promoting macrophage M2 polarization and elevating interleukin-10 (IL-10) expression. In vivo experiments further confirmed in vitro results, indicating that liver metastases elevated by ORM1 were partially attenuated by the depletion of macrophages or IL-10. Considered together, ORM1 promotes CRC progression and liver metastasis by regulating tumor cell growth and inducing macrophage M2 polarization, which mediates tumor immune tolerance, and thus acts as a potential predictive marker and therapeutic target in CRLM.
Physics informed neural network for charged particles surrounded by conductive boundaries
Authors: Hafezianzade Fatemeh , Biagooi Morad , Oskoee SeyedEhsan Nedaaee 
Published: August 2023

AbstractMolecular dynamics of charged particles in porous conductive media have received considerable attention in recent years due to their application in cutting-edge technologies such as batteries and supercapacitors. Due to the presence of long-range electrical interactions, induced charges present at the boundary, and the influence of boundary conditions, the simulation of these systems is more challenging than the simulation of typical molecular dynamic systems. Simulating these kinds of systems typically involves using a numerical solver to solve the Poisson equation, which is a very time-consuming procedure. Recently, Physics-Informed Neural Networks (PINNs) have been introduced as an alternative to numerical solutions of PDEs. In this paper, we present a new PINN-based model for predicting the potential of point-charged particles surrounded by conductive walls. As a result of the proposed PINN model, the mean square error is less than $$7\%$$

7
%

and $$R^{2}$$

R
2

score is more than $$90\%$$

90
%

for the corresponding example simulation. Results have been compared with typical neural networks and random forest as standard machine learning algorithms. The $$R^{2}$$

R
2

score of the random forest model was $$70\%$$

70
%

, and a standard neural network could not be trained well.
Structural equation modelling of work related musculoskeletal disorders among dumper operators
Authors: Kar Mohith Bekal , Aruna Mangalpady , Kunar Bijay Mihir 
Published: August 2023

AbstractThe aim of this study is to investigate the impact of personal factors, habitual factors, and work-related factors on work-related musculoskeletal disorders (WRMSDs) among dumper operators. In total, 248 dumper operators working in an iron ore mine were considered for this study. A questionnaire was developed and administered to collect dumper operators' personal, habitual, and work-related data. The reliability of the questionnaire was cross-checked by Cronbach alpha and the test–retest method. The values of Cronbach alpha for all latent variables were above 0.7, and the correlation coefficient of the questionnaire items at Time 1 and Time 2 was above 0.82. After verifying the validity (i.e., convergent and divergent) of the questionnaire data, the relationship between the factors under consideration was examined by structural equation modeling (SEM). The SEM demonstrated a moderate fit, with $$\frac{{\upchi }^{2}}{df}$$

χ

2

df

value of 1.386, comparative fit index (CFI) of 0.86, goodness-of-fit index (GFI) of 0.72, adjusted goodness-of-fit index (AGFI) of 0.69, Tucker-Lewis Index (TLI) of 0.83, normed fit index (NFI) of 0.71 and root mean square error of approximation (RMSEA) of 0.051. The SEM analysis revealed a positive relationship between WRMSDs and personal factors (with path coefficient = 0.313 and p < 0.05) as well as work-related factors (with path coefficient = 0.296 and p < 0.05). However, the relationship between WRMSDs and habitual factors was not statistically significant (with path coefficient = 0.142 and p > 0.05). Overall, this study provides valuable insights into the factors that influence the prevalence of WRMSDs among dumper operators. The findings highlight the significance of personal and work-related factors by which one can make a positive decision to prevent and reduce the incidence of WRMSDs among dumper operators.
Pictorial adaptation of the quality of recovery 15 scale and psychometric validation into a pediatric surgical population
Authors: Noll Eric , De Angelis Vincent , Bopp Claire , Chauvin Chloe , Talon Isabelle , Bennett-Guerrero Elliott , Lefebvre François , Pottecher Julien 
Published: August 2023

AbstractPatient reported outcomes measures (PROMS) are important endpoints to measure patient health status in the perioperative setting. However, there are no good tools to measure PROMS in the pediatric surgical population. Patients 7 to 17 years old undergoing surgery were included and followed up for 1 day after surgery (POD1). At POD1 the patients were asked to rate their overall postoperative recovery using a 100-mm visual analog scale (VAS). The primary outcome was the pediatric QoR-15 score on postoperative day 1 (POD1). 150 patients completed the study. The mean (SD) pediatric QoR-15F scores were 132.1 (14.1) and 111.0 (27.0), preoperatively and on POD1, respectively. Convergent validity confirmed with Pearson (r) correlation between the postoperative pediatric QoR-15F and the patient-rated global recovery assessment was 0.72 (95% confidence interval [0.63–0.79]; p < 10–16). Concerning reliability, internal consistency of the pediatric QoR-15 assessed by Cronbach’s alpha was 0.90. The test–retest concordance correlation coefficient was 0.92; 95% CI [0.83–0.96]. Split-half alpha was 0.74. The pictorial pediatric version of the QoR-15F showed good validity, reliability, responsiveness, acceptability and feasibility. This PROMS should be considered for clinical care and research in the perioperative pediatric patient setting.Trial Registration: NCT04453410 on clinicaltrials.gov.
Green synthesis of copper oxide nanoparticles and its efficiency in degradation of rifampicin antibiotic
Authors: Nzilu Dennis Mwanza , Madivoli Edwin Shigwenya , Makhanu David Sujee , Wanakai Sammy Indire , Kiprono Gideon Kirui , Kareru Patrick Gachoki 
Published: August 2023

AbstractIn recent ages, green nanotechnology has gained attraction in the synthesis of metallic nanoparticles due to their cost-effectiveness, simple preparation steps, and environmentally-friendly. In the present study, copper oxide nanoparticles (CuO NPs) were prepared usingParthenium hysterophoruswhole plant aqueous extract as a reducing, stabilizing, and capping agent. The CuO NPs were characterized via UV–Vis Spectroscopy, Fourier Transform Infrared Spectroscopy (FTIR), powder X-Ray Diffraction (XRD), Scanning Electron Microscopy (SEM), Transmission Electron Microscopy (TEM), and Dynamic Light Scattering (DLS). The UV–Vis spectra of CuO NPs showed a surface plasmonic resonance band to occur at 340 nm. FTIR analysis revealed the presence of secondary metabolites on the surface of CuO NPs, with a characteristic Cu–O stretching band being identified at 522 cm−1. Scanning electron micrographs and transmission electron micrographs showed that CuO NPs were nearly spherical, with an average particle of 59.99 nm obtained from the SEM micrograph. The monoclinic crystalline structure of CuO NPs was confirmed using XRD, and crystallite size calculated using the Scherrer-Debye equation was found to be 31.58 nm. DLS showed the presence of nanoparticle agglomeration, which revealed uniformity of the CuO NPs. Furthermore, the degradation ability of biosynthesized nanoparticles was investigated against rifampicin antibiotic. The results showed that the optimum degradation efficiency of rifampicin at 98.43% was obtained at 65℃ temperature, 50 mg dosage of CuO NPs, 10 mg/L concentration of rifampicin solution, and rifampicin solution at pH 2 in 8 min. From this study, it can be concluded that CuO NPs synthesized fromParthenium hysterophorusaqueous extract are promising in the remediation of environmental pollution from antibiotics. In this light, the study reports thatParthenium hysterophorus-mediated green synthesis of CuO NPs can effectively address environmental pollution in cost-effective, eco-friendly, and sustainable ways.