bluuebunny/crossref_metadata_2025_split huggingface dataset:
Real‐world data on the efficacy and safety of immune‐checkpoint inhibitors in elderly patients with non‐small cell lung cancer
Authors: Morinaga Daisuke , Asahina Hajime , Ito Shotaro , Honjo Osamu , Tanaka Hisashi , Honda Ryoichi , Yokouchi Hiroshi , Nakamura Keiichi , Takamura Kei , Hommura Fumihiro , Kawai Yasutaka , Ito Kenichiro , Sukoh Noriaki , Yokoo Keiki , Morita Ryo , Harada Toshiyuki , Takashina Taichi , Goda Tomohiro , Dosaka‐Akita Hirotoshi , Isobe Hiroshi ,   the Hokkaido Lung Cancer Clinical Study Group Trial
Published: March 2023

AbstractPurposeImmune‐checkpoint inhibitors (ICIs) are effective against advanced non‐small cell lung cancer (NSCLC). However, whether the efficacy and safety of ICI treatment in elderly patients are similar to those in younger patients is unclear. This study was designed to address this question.MethodsWe enrolled patients who received ICI monotherapy in Japan between December 2015 and December 2017; those ≥75 years of age comprised the elderly group. We compared the efficacy and safety of ICI monotherapy in elderly patients with those in younger patients and explored prognostic factors in elderly patients.ResultsWe enrolled 676 patients; 137 (20.3%) were assigned to the elderly group. The median age of the elderly and younger groups was 78 (range, 75–85) and 66 (range, 34–74) years. The median progression‐free survival (4.8 months vs. 3.3 months, p = 0.1589) and median overall survival (12.3 months vs. 13.0 months, p = 0.5587) were similar between the elderly and younger groups. Multivariate analysis revealed that a significantly better OS in the elderly group was associated with better responses to first‐ or second‐line ICI treatment (p = 0.011) and more immune‐related adverse events (irAEs) (p = 0.02). IrAEs that led to ICI discontinuation occurred in 34 of 137 patients (24.8%) in the elderly group, and their survival was significantly higher than that in those who did not have irAEs.ConclusionICI is also effective in elderly NSCLC patients, and treatment discontinuation due to irAEs may be a good prognostic marker.
Refining the Dynamic Network of T2SS Endopilus Tip Heterocomplex Combining cw‐EPR and Nitroxide–GdIII Distance Measurements
Authors: Gerbaud Guillaume , Barbat Brice , Tribout Mathilde , Etienne Emilien , Belle Valérie , Douzi Badreddine , Voulhoux Romé , Bonucci Alessio 
Published: March 2023

AbstractThe type 2 secretion system (T2SS) is a bacterial nanomachine composed of an inner membrane assembly platform, an outer membrane pore and a dynamic endopilus. T2SS endopili are organized into a homo‐multimeric body formed by the major pilin capped by a heterocomplex of four minor pilins. The first model of the T2SS endopilus was recently released, even if structural dynamics insights are still required to decipher the role of each protein in the full tetrameric complex. Here, we applied continuous‐wave and pulse EPR spectroscopy using nitroxide‐gadolinium orthogonal labelling strategies to investigate the hetero‐oligomeric assembly of the minor pilins. Overall, our data are in line with the endopilus model even if they evidenced conformational flexibility and alternative orientations at local scale of specific regions of minor pilins. The integration of different labelling strategies and EPR experiments demonstrates the pertinence of this approach to investigate protein–protein interactions in such multiprotein heterocomplexes.
Herd behaviors in index futures trading: Driving factors and impact on market volatility
Authors: Wu Ming‐Hung , Hu Wan‐Ting , Weng Pei‐Shih 
Published: March 2023

AbstractThis study analyzes market index futures data on the Taiwan Futures Exchange to explore herd trading behaviors and their impact on the market. The study finds that herd behaviors are prevalent in all trading directions and that investor sentiment is a more direct explanation than information chasing. Herding in intraday trading increases market volatility on the same day but decreases it on the following day. Additional tests show that overnight trading has a weaker herding tendency and a less noticeable market correction on the following day, which can be attributed in part to the lower participation of retail traders in overnight sessions. The analysis using overnight trading reinforces the relevance of retail trading in explaining herd trading. Overall, the study offers new evidence and insights into herd behaviors in the derivatives market dominated by retail investors.
Fly strike in sheep: updates on the cost of control
Authors: Lihou Katie , Lovatt Fiona , Wall Richard 
Published: March 2023

Background: Blowfly strike is an important seasonal problem which costs the UK sheep industry millions of pounds per year. To maximise margins, it is important that practitioners advise clients on the most cost‐effective treatment strategy for their flock.Aim of the article: This article models the costs associated with different strategies for the management of blowfly strike on sheep farms. Farm vets should use this model to determine the most appropriate strategy for their clients.
RETRACTED: Enhanced chemotherapeutic efficacy of docetaxel in human lung cancer cell line via GLUT1 inhibitor
Authors: Bahremani Mona , Rashtchizadeh Nadereh , Sabzichi Mehdi , Vatankhah Amir Mansour , Danaiyan Sepideh , Poursistany Haniyeh , Mohammadian Jamal , Ghorbanihaghjo Amir 
Published: March 2023

AbstractThe dose‐dependent adverse effects of anticancer agents need new methods with lesser toxicity. The objective of the current research was to evaluate the efficacy of GLUT1 inhibitor, as an inhibitor of glucose consumption in cancer cells, in augmenting the efficiency of docetaxel with respect to cytotoxicity and apoptosis. Cell cytotoxicity was assessed by using methylthiazolyldiphenyl‐tetrazolium bromide (MTT) assay. Annexin V/PI double staining was employed to evaluate apoptosis percentage. Quantitative real‐time polymerase chain reaction (RT‐PCR) analysis was accomplished to detect the expression of genes involved in the apoptosis pathway. The IC50 values for docetaxel and BAY‐876 were 3.7 ± 0.81 and 34.1 ± 3.4 nM, respectively. The severity of synergistic mutual effects of these agents on each other was calculated by synergy finder application. It showed that the percentage of apoptotic cells following co‐administration of docetaxel and BAY‐876 increased to 48.1 ± 2.8%. In comparison without GLUT1 co‐administration, the combined therapy decreased significantly the transcriptome levels of the Bcl‐2 and Ki‐67 and a remarkable increase in the level of the Bax as proapoptotic protein(p < 0.05). Co‐treatment of BAY‐876 and docetaxel depicted a synergistic effect which was calculated using the synergy finder highest single agent (HSA) method (HSA synergy score: 28.055). These findings recommend that the combination of GLUT‐1 inhibitor and docetaxel can be considered as a promising therapeutic approach for the treatment of patients with lung cancer.
Ophiopogonin D attenuates the progression of murine systemic lupus erythematosus by reducing B cell numbers
Authors: Nie Yingkun , Li Chunyan , Sun Ni 
Published: March 2023

AbstractSystemic lupus erythematosus (SLE) is a chronic autoimmune disease characterized by immune abnormalities leading to multi‐organ damage. The activation of autoreactive B cell differentiation will lead to the production of pathogenic autoantibodies, contributing to the development of SLE. However, the effects of Ophiopogonin D (OP‐D) on B cell activation and autoantibody production as well as renal injury in the pathogenesis of SLE remain unclear. MRL/lpr mice, one of the most commonly used animal models of SLE, were intragastrically administered with 5 mg/kg/d OP‐D at 17 weeks of age for 3 weeks. The survival rates of mice in each group were monitored for 6 weeks until 23 weeks of age. Proteinuria and serum creatinine levels were measured. Serum levels of immunoglobulin (Ig)G, IgM, and anti‐dsDNA autoantibodies were detected by enzyme‐linked immunosorbent assay. Numbers of CD19+ B cells in the blood, spleen and bone marrow and numbers of splenic germinal center (GC) B cells were calculated by using flow cytometry. OP‐D treatment prolonged survival in MRL/lpr mice. OP‐D treatment reduced proteinuria and serum creatinine levels as well as mitigated renal pathological alternation in MRL/lpr mice. Furthermore, serum levels of IgG, IgM, and anti‐dsDNA autoantibodies were reduced by OP‐D treatment. OP‐D lessened not only CD19+ B cells in the spleen and bone marrow but also plasma cells that secreted anti‐dsDNA autoantibodies, IgG and IgM in the spleen and bone marrow. OP‐D ameliorated the progression of SLE by inhibiting the secretion of autoantibodies though reducing B cell numbers.
Integrated transcriptome, proteome and single‐cell sequencing uncover the prognostic and immunological features of colony‐stimulating factor 3 receptor in pan‐cancer
Authors: Song Liying , Jiang Feng , Tian Yu , Cao Xiaolan , Zhu Minxia , Zhang Jie , Wang Xiaoping , Deng Langmei 
Published: March 2023

AbstractBackgroundColony‐stimulating factor 3 receptor (CSF3R) has been demonstrated to be associated with various hematological tumors, especially chronic neutrophilic leukemia; however, the detailed roles of CSF3R in other cancers remain to be explored.MethodsIn the present study, we systematically analyzed the expression profiles of CSF3R in pan‐cancer by comprehensive bioinformatics databases, such as Tumor Immune Estimation Resource, version 2 (TIMER2.0), Gene Expression Profiling Interactive Analysis, version 2 (GEPIA2.0), etc. GEPIA2.0 was also used to analyze the relationship between CSF3R expression and patients' survival prognosis.ResultsWe found that the high expression of CSF3R was associated with a poor prognosis in the brain tumor patients, such as brain lower grade glioma and glioblastoma multiforme. In addition, we further investigated the genetic mutation and DNA methylation level of CSF3R in multiple cancers. Immune infiltration analysis showed that CSF3R expression was positively correlated with a variety of tumor‐infiltrating immune cells in most cancers. Single cell sequencing indicated that CSF3R levels were correlated with several cancer‐associated pathways, such as DNA damage, cell invasion, and stemness.ConclusionsTaken together, the role of CSF3R in multiple cancers might reveal its potential as a novel prognostic biomarker and therapeutic target for cancer patients.
Treatment outcomes of extraskeletal Ewing sarcoma
Authors: Houdek Matthew T. , Heidenreich Mark J. , Ahmed Safia K. , Allen‐Rhoades Wendy , Siontis Brittany L. , Robinson Steven I. , Petersen Ivy A. , Rose Peter S. 
Published: March 2023

AbstractPurposeExtraskeletal Ewing sarcoma (EES), is a rare soft tissue sarcoma. Treatment for EES commonly involves chemotherapy and surgical resection (ST) or less commonly combined chemotherapy, surgery, and radiotherapy (ST + RT). The purpose of the current study was to evaluate our institutional experience treating EES.MethodsWe reviewed 36 (18 males:18 females) patients (mean age 30 years) with a nonretroperitoneal/visceral EES treated with either ST (n = 24, 67%) or ST + RT (n = 12, 33%). All patients were treated with chemotherapy, most commonly vincristine, doxorubicin, cyclophosphamide/ifosfamide and etoposide (VDC/IE, n = 23, 66%) Radiotherapy was mostly delivered preoperatively (n = 9). The mean follow‐up was 8 years.ResultsThe 10‐year disease specific survival for patients was 78%, with no difference in the survival between patients in the ST versus the ST + RT groups (83% vs. 71%, p = 0.86). There was no difference in the 10‐year local recurrence (91% vs. 100%, p = 0.29) or metastatic free survival (87% vs. 75%, p = 0.45) between the ST and ST + RT groups.ConclusionThe results of the current study highlight the ability to achieve excellent local control with chemotherapy and surgery for EES. We recommend for multidisciplinary management of patients with EES, including chemotherapy and surgery, with use of radiotherapy if there is concern for a potentially close margin of resection.
Neoadjuvant chemotherapy associated with isotoxic high‐dose stereotactic body radiotherapy does not increase postoperative complications after pancreaticoduodenectomy for nonmetastatic pancreatic cancer
Authors: Navez Julie , Bouchart Christelle , Mans Laura , Devos Soline , Loi Patrizia , Closset Jean , Van Laethem Jean‐Luc 
Published: March 2023

AbstractBackground and ObjectivesThe role of radiotherapy in the therapeutic sequence of nonmetastatic pancreatic cancer (PC) is controversial, including isotoxic high‐dose stereotactic body radiotherapy (iHD‐SBRT). This study aimed to compare postoperative outcome of patients with nonmetastatic PC undergoing neoadjuvant treatment (NAT) including iHD‐SBRT versus upfront pancreaticoduodenectomy (PD).MethodsAll patients undergoing PD for PC from 2017 to 2021 were retrospectively analyzed, identifying patients receiving NAT with iHD‐SBRT. Toxicity of treatments and postoperative outcome were assessed and analyzed in a propensity score‐matched population.ResultsEighty‐nine patients underwent upfront surgery (surgery group) and 22 after NAT and iHD‐SBRT (SBRT group). No major side effects SBRT‐related were identified preoperatively. Postoperative morbidity was similar between groups. There was no postoperative death in SBRT group, and six in surgery group (p = 0.597). No difference was observed in the rates of complications related to pancreatic surgery. The postoperative hospital stay was shorter in SBRT versus surgery groups (p = 0.016). After propensity score matching, no significant difference in the postoperative morbidity was observed between groups.ConclusionsIncorporation of iHD‐SBRT in the NAT sequence before PD for PC did not increase postoperative morbidity compared with upfront surgery. These results confirm the feasibility and safety of iHD‐SBRT for the upcoming STEREOPAC trial.
Surgical management of dermatofibrosarcoma protuberans
Authors: Rust Dylan J. , Kwinta Bradley D. , Geskin Larisa J. , Samie Faramarz H. , Remotti Fabrizio , Yoon Sam S. 
Published: March 2023

AbstractDermatofibrosarcoma protuberans (DFSP) is an uncommon, locally aggressive cutaneous malignancy. Complete resection is the primary treatment but there is debate over the optimal method. Wide local excision was traditionally the standard of care; however, National Comprehensive Cancer Network guidelines now recommend Mohs micrographic surgery as the preferred approach. Medical therapy with imatinib can be used in advanced or unresectable disease. This review will discuss the current management of DFSP, focusing on optimal surgical approach.
Macrophages facilitate tumor cell PD‐L1 expression via an IL‐1β‐centered loop to attenuate immune checkpoint blockade
Authors: Xu Cheng , Xia Yu , Zhang Bai‐Wei , Drokow Emmanuel Kwateng , Li Hua‐Yi , Xu Sen , Wang Zhen , Wang Si‐Yuan , Jin Ping , Fang Tian , Xiong Xiao‐Ming , Huang Pu , Jin Ning , Tan Jia‐Hong , Zhong Qing , Chen Yu‐Xin , Zhang Qi , Fang Yong , Ye Fei , Gao Qing‐Lei 
Published: March 2023

AbstractTumor‐associated macrophages (TAMs) play critical roles in reprogramming other immune cells and orchestrating antitumor immunity. However, the interplay between TAMs and tumor cells responsible for enhancing immune evasion remains insufficiently understood. Here, we revealed that interleukin (IL)‐1β was among the most abundant cytokines within the in vitro tumor‐macrophage coculture system, and enhanced IL‐1β expression was associated with impaired cytotoxicity of CD8+ T cells in human ovarian cancer, indicating the possibility that IL‐1β mediated immunosuppression during tumor‐TAMs crosstalk. Mechanistically, we demonstrated that IL‐1β significantly boosted programmed death‐ligand 1 (PD‐L1) expression in tumor cells via the activation of the nuclear factor‐κb signaling cascade. Specifically, IL‐1β released from TAMs was triggered by lactate, the anaerobic metabolite of tumor cells, in an inflammasome activation‐dependent manner. IL‐1β sustained and intensified immunosuppression by promoting C‐C motif chemokine ligand 2 secretion in tumor cells to fuel TAMs recruitment. Importantly, IL‐1β neutralizing antibody significantly curbed tumor growth and displayed synergistic antitumor efficacies with anti‐PD‐L1 antibody in tumor‐bearing mouse models. Together, this study presents an IL‐1β‐centered immunosuppressive loop between TAMs and tumor cells, highlighting IL‐1β as a candidate therapeutic target to reverse immunosuppression and potentiate immune checkpoint blockade.
Preparation of polyaniline/cellulose nanofiber composites with enhanced anticorrosion performance for waterborne epoxy resin coatings
Authors: Zhao Yuxiang , Huang Minggang , Gao Zhiwei , He Heng , Chen Yan , He Feng , Lin Yi , Yan Bin , Chen Sheng 
Published: March 2023

AbstractIn this work, polyaniline‐cellulose nanofiber (PANI‐CNF) nanocomposites were prepared by in‐situ polymerization of aniline on 2,2,6,6‐tetramethyl‐1‐piperidinyloxy (TEMPO) oxidized CNFs. Fourier transform infrared spectroscopy and transmission electron microscope demonstrate the successful polymerization of aniline on CNFs. The PANI‐CNF nanocomposites were added as nanofillers to waterborne epoxy resin (WER) in different proportions. The composite coatings with different contents of PANI‐CNFs were prepared by fabricating the mixture on the Q235 steel sheets. The corrosion resistance of different coatings was evaluated by potentiodynamic polarization and electrochemical impedance spectroscopy. The results confirmed that composite coatings containing 0.5% PANI‐CNF exhibit the optimum anticorrosion behaviors and best corrosion resistance, which shows the lowest corrosion current density (1.788 × 10−7 A/cm−2) and highest corrosion potential (−0.458 V). In addition, PANI‐CNF can significantly enhance the anticorrosion performance of WER coatings and maintain a high value even after 40 days of immersion in NaCl solutions. The above performances are attributed to the synergistic effect of the barrier effects and the passivation mechanism. Therefore, the bio‐based composite materials developed in this work are promising in enhancing the corrosion protection of mild steel, which is essential for the sustainable development of waterborne coatings.
H∞ Control design for discrete‐time nonlinear delayed systems
Authors: Subramaniyam Ramasamy , Joo Young Hoon 
Published: March 2023

SummaryThis manuscript concerns the stabilization of discrete‐time Takagi‐Sugeno (T‐S) fuzzy systems with additive time‐varying delay components. By constructing a suitable fuzzy Lyapunov‐Krasovskii functional (LKF) with membership functions, the sufficient condition of the stability criteria has been established for the discrete‐time T‐S fuzzy systems with additive time‐varying delays. To do this, the extended version of Wirtinger's inequality is used to bound the forward differences of the LKF. By utilizing the T‐S fuzzy model approach, the dynamics of each fuzzy rule is expressioned by a linear system. Then, a fuzzy controller is designed to guarantee the performance of the closed‐loop system formulated in terms of linear matrix inequalities (LMIs). Finally, the numerical simulations are carried out to demonstrate the effectiveness and feasibility of our theoretical findings.
Size‐Dependent Electrochemistry of Oxygenated Ti3C2Tx MXenes
Authors: Lei Ling , Yin Jiaxi , Wu Kangbing , Yang Nianjun 
Published: March 2023

Abstract2D MXenes are widely proved to be potential electrode materials, although the size effect on their electrochemistry is not fully understood. In this work, Ti3C2Tx nanoflakes are prepared through acidic etching of Ti3AlC2 powders, followed by the intercalation treatment with tetrapropylammonium hydroxide. Such a method produces large‐scale delaminated and oxygenated nanoflakes. With aid of centrifugation, the nanoflakes with varied lateral sizes and thicknesses are collected, where electrochemical response of charged redox probes and polar phenol molecules is varied. Density functional theory and energy dispersive spectroscopy confirm such electrochemical response is dependent on the size and thickness of used nanoflakes, more exactly the oxygen content on their surface. Taking the nanoflakes obtained using a centrifugal speed of 5000 rpm (MX‐TPA0.2) as an example, they feature good dispersibility, a high oxygen content, a small size, and a thin thickness. On these nanoflakes electrochemical response of polar p‐substituted phenols is pronounced, stemming from a strong electron‐withdrawing interaction of their oxygenated termination with the Ar‐OH. A sensitive electrochemical sensor is further constructed for the detection of p‐nitrophenol. This work thus provides an approach to synthesize MXenes with different sizes and thicknesses as well as further to reveal size‐dependent electrochemistry of MXenes.
Inhibition of sepsis‐induced acute kidney injury via thecircITCH‐miR‐579‐3p‐ZEB2axis
Authors: Li Qing , Wang Tianyi , Wang Ximin , Ge Xin‐Yu , Yang Tao , Bai Guang , Wang Wei 
Published: March 2023

AbstractCircular RNAs (circRNAs) are linked to the regulation of sepsis‐induced acute kidney injury (AKI). However, the function of circITCH in the development of sepsis‐induced AKI is still unclear. The levels of circITCH, miR‐579‐3p and ZEB2 were examined by real‐time PCR and immunoblotting. Then, the roles of circITCH in cell viability, apoptosis, and inflammation in lipopolysaccharide (LPS)‐treated HK‐2 cells were evaluated. The further mechanism was investigated using rescue assays. CircITCH was downregulated in septic AKI patients and LPS‐triggered HK‐2 cells. CircITCH overexpression restored cell viability in LPS‐treated HK‐2 cells and restrained apoptosis and inflammatory cytokine production. CircITCH negatively regulated miR‐579‐3p, thereby upregulating ZEB2 expression. Taken together, circITCH alleviates LPS‐induced HK‐2 cell injury by regulating miR‐579‐3p/ZEB2 signal axis, which provides a theoretical basis for AKI therapy.
Making Reference Genomes Useful: Annotation
Authors: García-Sancho Miguel , Lowe James 
Published: March 2023

AbstractThrough examining how the reference genomes of yeast, human and pig were annotated, in this chapter we further identify how the involvement or non-involvement of particular communities in the creation of a reference genome can affect the nature of the product. We therefore continue to distinguish the qualities of separate reference genomes, which are otherwise rendered as commensurate and equivalent objects by data repositories such as RefSeq. In doing so, we present alternative historical trajectories to the narrative centred around the Human Genome Project. In particular, this chapter conveys how the relationship between pig genomicists and a key annotation group at the Sanger Institute shaped the direction of annotation at that institution. This led the Sanger Institute group to formulate a new way of conducting ‘community annotation’, an approach that had previously been performed in a limited and attenuated fashion, for example at the jamboree meetings of Celera Genomics.
Improving and Going Beyond Reference Genomes
Authors: García-Sancho Miguel , Lowe James 
Published: March 2023

AbstractIn this chapter, we show that many of the features of ‘post-genomics’ were present in pre-reference genome research, and the reference genomics of yeast and pig. Due to the problems we identify with the notion of ‘post-genomics’, we instead propose the term ‘post-reference genomics’, which encompasses all the forms of genomic-related research opened up by the existence of a reference sequence. To identify what is distinct about post-reference genomics, we detail the relationship between two modes of research: functional and systematic. We observe how the evolving relationship between these two modes of research differs across species, and attribute this to distinct relationships between scientific communities and the pre-reference genomics or reference genomics work they were involved in. We close by considering the role of reference genomes and other genomic resources in seeding ‘webs of reference’ that enable researchers and other practitioners to explore the possible variation exhibited by a given species.