bluuebunny/crossref_metadata_2025_split huggingface dataset:
Targeting XPO6 inhibits prostate cancer progression and enhances the suppressive efficacy of docetaxel
Authors: Wang Huming , Teng Xiangyu , Lin Yuan , Jiang Chao , Chen Xin , Zhang Ying 
Published: May 2023

Abstract
Background
Although XPO6, one of the Exportin family members, functions in malignant progression of certain types of cancer, its role in prostate cancer (PCa) has not been elucidated. Herein, we investigated the oncogenic effect and clarified the downstream mechanism of XPO6 in PCa cells.

Methods
We detected the expression level of XPO6 in PCa tissues by immunohistochemistry (IHC) and analyzed the correlation between clinicopathological characteristics and XPO6 level based on TCGA database. The effects of XPO6 in the proliferation and migration or resistance to docetaxel (DTX) in PCa cells were assessed using CCK8, colony formation, wound-healing and Transwell assays. Mice experiments were performed to investigate the role of XPO6 in tumor progression and DTX effect in vivo. Further, functional analysis of DEGs revealed the correlation of XPO6 with Hippo pathway and XPO6 could promote the expression and nuclear translocation of YAP1 protein. Furthermore, blocking Hippo pathway with YAP1 inhibitor leads to the loss of XPO6-mediated regulation of biological functions.

Results
XPO6 was highly expressed and positively correlated with the clinicopathological characteristics of PCa. Functional experiments indicated that XPO6 could promote tumor development and DTX resistance in PCa. Mechanistically, we further confirmed that XPO6 could regulate Hippo pathway via mediating YAP1 protein expression and nuclear translocation thereby promoting PCa progression and chemotherapeutic resistance.

Conclusion
In conclusion, our research reveals that XPO6 potentially function as an oncogene and promotes DTX resistance of PCa, suggesting that XPO6 could be both a potential prognostic marker as well as a therapeutic target to effectively overcome DTX resistance.
Water disinfection using durable ceramic filter coated with silver nanoparticles synthesized using actinomycetes
Authors: Wafy Karam Rabee , El-Aswar Eslam Ibrahim , Mohamed Walaa Salah El-din , El-Sabbagh Sabha Mahmoud 
Published: May 2023

AbstractContamination with pathogens degrades water quality and is a major cause of many waterborne diseases. The aim of this research is to reduce the global disease burden by presenting an efficient, durable, and low-cost ceramic filter impregnated with actinomycetes-mediated silver nanoparticles (AgNPs) for water disinfection in rural areas. This marks the first report on the simultaneous biosynthesis of AgNPs utilizing cell-free supernatants obtained from terrestrial actinomycetes. An easy and efficient method was used to impregnate AgNPs onto a ceramic filter using 3-aminopropyltriethoxysilane (APTES). The APTES linker is anchored to the ceramic surface through Si–O–Si bridges, while the terminal amino groups coordinate with AgNPs. Notably, the observed inhibition zone around the filter with AgNPs was ~ 18 mm, suggesting that the silver ions were responsible for the antibacterial activity. After 30 min of sonication, only insignificant traces of AgNPs were released from the filter, making it stable for long-term antibacterial activity when treating water. According to the laboratory simulation experiments, the untreated filter can reject about 99% of spiked bacteria, while the antibacterial efficiency of the filter coated with AgNPs was 100% due to the synergistic effect between filtration and disinfection with AgNPs. In addition, the average concentration of dissolved silver in the outlet water of the ultrafiltration system during three months was 33.7 μg/L, far below the permissible limit (100 μg/L) for drinking water. Overall, this work offers a suitable and affordable water treatment strategy for low-income, isolated, and rural societies in developing countries.
Comparison of classic time series and artificial intelligence models, various Holt-Winters hybrid models in predicting the monthly flow discharge in Marun dam reservoir
Authors: Ahmadpour Abbas , Haghighat Jou Parviz , Mirhashemi Seyed Hassan 
Published: May 2023

AbstractIn this study, the data at Idenak hydrometric station were used to predict the inflow to Maroun Dam reservoir. For this purpose, different models such as artificial intelligence, Holt-Winters and hybrid models were used. Partial mutual information algorithm was used to determine the input parameters affecting modeling the monthly inflow by artificial intelligence models. According to the Hempel and Akaike information criterion, we introduced the monthly inflow with a 3-month lag, and the temperature with a 1-month lag, with respect to the lowest values of Akaike and the highest values of Hempel as input parameters of artificial intelligence models. The results showed the weak performance of the Holt-Winters model compared to other models and confirmed the superiority of the Holt-adaptive network-based fuzzy inference system (ANFIS) hybrid model with the root-mean-square error of 54 and the coefficient of determination (R2) of 0.83 in the testing process compared to other mentioned models. In addition, the above hybrid models performed better than other models in the test process.
Condensation distribution and evolution characteristics of water vapor in annulus of flexible riser
Authors: Mao Gangtao , Zhao Bingliang , Wang Kai 
Published: May 2023

AbstractWater vapor penetrating an annulus causes condensation and corrosion and endangers the safe operation of the flexible riser. However, in current studies, the condensation of water vapor in the annulus is modeled based on the film condensation hypothesis, which assumes that the metal material is uniformly corroded. This assumption differs from the true corrosion incidents, which are usually initiated by localized corrosion. To further study the condensation law of water vapor in a flexible riser annulus, the evolution of water vapor condensation on the wall surface of a sapphire reactor was evaluated using an annular condensation experimental device. A water vapor condensation growth model was established to analyze the condensation law and its influencing factors. The results show that the maximum radius of condensate droplets on the annular wall surface is approximately 0.48 cm, the average radius of droplets is approximately 0.35 cm, and the volume of condensate that can be accommodated in the 18 × 18 mm simulation area is approximately 0.5 cm3. After the droplet reaches the departure radius and falls off a surface, the time of recondensation is faster than that of the first condensation. The condensation surface coverage increases rapidly in the early stage and then gradually slows down. After stabilization, the maximum coverage can reach 80%. Parameter analysis suggests that the number of fixed nucleation points on the condensation wall affects the time to reach the departure radius and the increasing rate of surface coverage. However, the number of fixed nucleation points has minimal influence on the departure radius and coverage outcomes. The findings of this study establish a theoretical foundation for predicting dropwise condensation behaviors in annulus of pipelines and developing corrosion protection strategies that modify the wall contact angle or structure.
Neuroendocrine response to diclofenac in healthy subjects: a pilot study
Authors: Varaldo E. , Sibilla M. , Bioletto F. , Cuboni D. , Prencipe N. , Bona C. , Ferrari M. , Viglino F. , Aversa L. S. , Grottoli S. , Ghigo E. , Gasco V. , Berton A. M. 
Published: May 2023

Abstract
Purpose
The precise effects of non-steroidal anti-inflammatory drugs on the neuroendocrine hydro-electrolytic regulation are not precisely understood. The aim of this pilot study was to evaluate, in healthy subjects, the neuroendocrine response of the antidiuretic system to intravenous diclofenac infusion.

Methods
For this single-blinded, cross-over study, we recruited 12 healthy subjects (50% women). Test sessions were divided into three observation times (pre-test; test; 48 h post-test), which were repeated equally on two different occasions, with the administration of diclofenac (75 mg in saline solution 0.9% 100 cc) on 1 day, or placebo (saline solution 0.9% 100 cc) on another day. The night before the test the subjects were asked to collect a salivary cortisol and cortisone sample, which was repeated on the night of the procedure session. Serial urine and blood samples were collected on the test day (for osmolality, electrolytes, ACTH, cortisol, copeptin, MR-proADM, MR-proANP; the last three represent more stable and analytically reliable molecules than their respective active peptides). Moreover, the subjects were evaluated with the bioimpedance vector analysis (BIVA) before and after the test. Forty-eight hours after the end of the procedure urine sodium, urine potassium, urine osmolality, serum sodium and copeptin were revaluated together with BIVA.

Results
No significant changes in circulating hormone levels were observed; anyway, 48 h after diclofenac, BIVA showed a significant water retention (p < 0.00001), especially in extracellular fluid (ECF) (16.47 ± 1.65 vs 15.67 ± 1.84, p < 0.001). Salivary cortisol and cortisone tended to increase only the night after placebo administration (p = 0.054 cortisol; p = 0.021 cortisone).

Conclusion
Diclofenac resulted in an increased ECF at 48 h, but this phenomenon seems to be associated with a greater renal sensibility to the action of vasopressin rather than with an increase in its secretion. Moreover, a partial inhibitory effect on cortisol secretion can be hypothesized.
Quantifying the Soil Water Storage Capacity of Flysh Catchments Surrounded by Mixed Forests in Outer Carpathians
Authors: Halecki Wiktor , Łyszczarz Stanisław , Lasota Jarosław , Błońska Ewa , Chattopadhyay Somsubhra 
Published: May 2023

AbstractThe objective of this paper is to evaluate the retention capacity of catchments situated in the Beskid Wyspowy region of southern Poland. To accomplish this, we employed the Soil and Water Assessment Tool (SWAT+), a newly developed hydrological model. The large heterogeneity of the catchment area and the limitations of measurement techniques necessitated the use of hydrological models. The study found that forested and pasture areas had higher soil water retention potential than agricultural areas. Furthermore, surface runoff of both catchments correlated negatively with soil water retention potential and evapotranspiration, however, positively with mean annual precipitation. Soil moisture was directly related to mean annual precipitation and electrical conductivity. The research also advocates incorporating the Landscape Hydric Potential (LHP) index into existing hydrologic models, in line with the physical-based SWAT+. Future watershed models based on water balance should be developed to increase resilience to climate change manifestations in the flysh Carpathian Mountains.
Phylogenetic diversity of Clavibacter michiganensis subsp. michiganensis isolates causing bacterial canker of tomato in Greece
Authors: Malliarakis Dimitris , Pagoulatou Marianthi G. , Mpalantinaki Evaggelia , Trantas Emmanouil , Ververidis Filippos , Goumas Dimitrios E. 
Published: May 2023

AbstractTomato bacterial canker, caused by Clavibacter michiganensis subsp. michiganensis (Cmm) (Li et al. 2018), is a common destructive disease worldwide. The bacterium colonizes plant’s vascular system causing characteristic symptoms such as wilting, cankers, brown and black discoloration of vessels, white spots on the fruit and leaves. In the present work, isolates were collected from infected tomato plants of 11 Greek Prefectures during the years 2003-2018, and the genetic variability was investigated. After initial identification, 93 selected Cmm strains were subjected to phylogenetic analyses based on the sequencing of multiple loci (Multi-Locus Sequencing Analysis, MLSA) utilizing four housekeeping genes (atpD, ppk, kdpA and sdhA). MLSA highlighted 4.1% variability in nucleotide sequences and classified the 93 Cmm strains into 38 haplotypes. Nevertheless, the phylogenetic grouping of the strains was not in accordance with the geographical distribution, a fact that probably advocates the introduction of the disease from infected propagating material. However, strains isolated from the same area in a different year were placed in the same group which means that the pathogen can remain active between growing seasons. The study provides important epidemiological information on the disease outbreaks and the spread of Cmm in Greece.
A new load bearing capacity assessment method of the road pavement based on surface strain and the spectrum of the acceleration
Authors: Liu Xiaolan , Zhang Xianmin 
Published: May 2023

AbstractBased on the coupling res model of vehicular and road, the influences of surface layer modulus, surface layer thickness and equivalent modulus of elasticity on the spectrum of the acceleration, surface deflection under wheels, and surface strain at the distance of 0.4 m to 0.8 m away from wheels are analyzed. Results show that the fundamental frequency of the road pavement increases with the growth of surface layer modulus and equivalent modulus of elasticity, and decreases with the increase of surface layer thickness. Both surface deflections and surface strains decrease with the increase of equivalent modulus of elasticity as well as surface layer modulus and thickness. Moreover, when the equivalent modulus of elasticity is more than 2500 MPa and surface layer modulus is more than 4000 MPa, surface deflections and surface strains have a little variation with the enhance of the surface layer modulus and equivalent modulus of elasticity. The solution method of the spectrum of the acceleration and equivalent modulus of elasticity, surface deflection under wheels, surface strain at the distance of 0.4 m to 0.8 m away from wheels, and surface deflection based on surface strain are established. The load bearing capacity assessment method of the road pavement based on surface strain and the spectrum of the acceleration is provided. Finally, the feasibility and accuracy of this method is verified by comparing measured values in field testing. The method in this paper provides a theoretical support for the design, construction and maintenance of road engineering.
Defining Clinical Trial Estimands: A Practical Guide for Study Teams with Examples Based on a Psychiatric Disorder
Authors: Polverejan Elena , O’Kelly Michael , Hefting Nanco , Norton Jonathan D. , Lim Pilar , Walton Marc K. 
Published: May 2023

AbstractWhile the ICH E9(R1) Addendum on “Estimands and Sensitivity Analysis in Clinical Trials” was released in late 2019, the widespread implementation of defining and reporting estimands across clinical trials is still in progress and the engagement of non-statistical functions in this process is also in progress. Case studies are sought after, especially those with documented clinical and regulatory feedback. This paper describes an interdisciplinary process for implementing the estimand framework, devised by the Estimands and Missing Data Working Group (a group with clinical, statistical, and regulatory representation) of the International Society for CNS Clinical Trials and Methodology. This process is illustrated by specific examples using various types of hypothetical trials evaluating a treatment for major depressive disorder. Each of the estimand examples follows the same template and features all steps of the proposed process, including identifying the trial stakeholder(s), the decisions they need to make about the investigated treatment in their specific role and the questions that would support their decision making. Each of the five strategies for handling intercurrent events are addressed in at least one example; the featured endpoints are also diverse, including continuous, binary and time to event. Several examples are presented that include specifications for a potential trial design, key trial implementation elements needed to address the estimand, and main and sensitivity estimator specifications. Ultimately this paper highlights the need to incorporate multi-disciplinary collaborations into implementing the ICH E9(R1) framework.
Do the benefits of Opzelura (Ruxolitinib) outweigh the potential risks? An insight into the FDA-approved treatment for non-segmental vitiligo (NSV)
Authors: Iqbal Amna , Salman Madiha 
Published: May 2023

Abstract
Recently, Ruxolitinib (Opzelura) has been approved by the Food and Drug Administration (FDA) as the first treatment for non-segmental vitiligo, which results in chalky white macules with clear edges due to an autoimmune disorder. The mode of action of this cream is JAK inhibition which progressively enhances the growth of new, healthy skin cells, and eventually, repigmentation occurs in the affected area. Indeed, this medication represents significance in the field of dermatology as almost 3/4th % of facial vitiligo was resolved but the high cost will be a hindrance in facilitating the patients and should be subsidized. Moreover, prescribers and pharmacists should be aware of the black box warnings issued by FDA as severe caution is indicated. More clinical trials should be conducted to know the safety of children under 12 years of age.

Highlights
Proton Plasma Asymmetries Between Venus' Quasi‐Perpendicular and Quasi‐Parallel Magnetosheaths
Authors: Rojas Mata Sebastián , Stenberg Wieser Gabriella , Futaana Yoshifumi , Zhang Tielong 
Published: May 2023

AbstractProton plasma asymmetries between the hemispheres of Venus' dayside magnetosheath lying downstream of the quasi‐perpendicular (q⊥) and quasi‐parallel (q‖) sides of the bow shock are characterized using measurements taken by a mass‐energy spectrometer. This characterization enables comparison to analogous Earth studies, thereby providing insight as to which plasma phenomena, such as turbulent particle heating, contribute in creating the observed plasma asymmetries in planetary magnetosheaths. A database of dayside bow‐shock crossings along with magnetosheath proton densities, bulk speeds, temperatures, and magnetic‐field strengths is manually constructed by selecting measurements taken during stable solar‐wind conditions. Ratios of these magnetosheath proton parameters are calculated as functions of distance from the central meridian and the upstream Alfvén Mach number to quantify the q⊥/‖ asymmetries. The density and bulk‐speed exhibit q‖‐favored asymmetries, mirroring those observed at Earth, whereas the magnetic‐field strength reveals no significant asymmetry despite expectations based on simulations. The temperatures perpendicular (T⊥) and parallel (T‖) to the background magnetic field have q⊥‐favored asymmetries while the temperature anisotropy T⊥/T‖ exhibits a q‖‐favored asymmetry. This trend is opposite to that seen at Earth, suggesting that the different spatial scales of the two planets' magnetosheaths may affect the impact of turbulent processes on global plasma properties.
East‐West Variabilities of N2 Fixation Activity in the Subtropical North Pacific Ocean in Summer: Potential Field Evidence of the Phosphorus and Iron Co‐Limitation in the Western Area
Authors: Horii S. , Takahashi K. , Shiozaki T. , Takeda S. , Sato M. , Yamaguchi T. , Takino S. , Hashihama F. , Kondo Y. , Takemura T. , Furuya K. 
Published: May 2023

AbstractIn the subtropical North Pacific, the east‐west gradient of iron deposition as atmospheric Asian dust strongly affects the zonal distribution of biological N2 fixation activity in numerical models, but the in‐situ relationship at a basin‐scale is not well examined. We examined the trans‐Pacific zonal variation in N2 fixation activity on 23°N in summer along with environmental parameters that potentially influence diazotrophy. Dissolved inorganic iron (DFe) was consistently low (<0.4 nM) throughout the transect. The atmospheric dust iron (dust‐Fe) flux increased westward, whereas phosphate and labile phosphoric monoesters in the surface decreased westward. N2 fixation varied between 34.6 and 298 μmol‐N m−2 d−1 and was high (>200 μmol‐N m−2 d−1) in the central area (150–180°W). N2 fixation rates significantly increased with dust‐Fe input only in the western area (137–180°E), whereas the contribution of DFe diffused from below the euphotic zone was often larger in the eastern area (120–170°W). N2 fixation was considerably low in the phosphate‐depleted western area despite the excess amount of iron relative to phosphate, and it increased with the labile phosphoric monoesters stock. These indicated that N2 fixation was primarily limited by phosphorus in the western area, though this activity also increased with iron supply from dust, likely due to phosphorus‐iron co‐limitation. In contrast, in the phosphorus‐repleted eastern area, iron supplied from dust and below the euphotic zone appeared to limit N2 fixation. Overall, N2 fixation in the subtropical North Pacific was likely limited by zonally different factors relating to iron and phosphorus availability.
Recent Advances and New Frontiers in Riverine and Coastal Flood Modeling
Authors: Jafarzadegan Keighobad , Moradkhani Hamid , Pappenberger Florian , Moftakhari Hamed , Bates Paul , Abbaszadeh Peyman , Marsooli Reza , Ferreira Celso , Cloke Hannah L. , Ogden Fred , Duan Qingyun 
Published: May 2023

AbstractOver the past decades, the scientific community has made significant efforts to simulate flooding conditions using a variety of complex physically based models. Despite all advances, these models still fall short in accuracy and reliability and are often considered computationally intensive to be fully operational. This could be attributed to insufficient comprehension of the causative mechanisms of flood processes, assumptions in model development and inadequate consideration of uncertainties. We suggest adopting an approach that accounts for the influence of human activities, soil saturation, snow processes, topography, river morphology, and land‐use type to enhance our understanding of flood generating mechanisms. We also recommend a transition to the development of innovative earth system modeling frameworks where the interaction among all components of the earth system are simultaneously modeled. Additionally, more nonselective and rigorous studies should be conducted to provide a detailed comparison of physical models and simplified methods for flood inundation mapping. Linking process‐based models with data‐driven/statistical methods offers a variety of opportunities that are yet to be explored and conveyed to researchers and emergency managers. The main contribution of this paper is to notify scientists and practitioners of the latest developments in flood characterization and modeling, identify challenges in understanding flood processes, associated uncertainties and risks in coupled hydrologic and hydrodynamic modeling for forecasting and inundation mapping, and the potential use of state‐of‐the‐art data assimilation and machine learning to tackle the complexities involved in transitioning such developments to operation.