bluuebunny/crossref_metadata_2025_split huggingface dataset:
Following Cu Microstructure Evolution in CuZnO/Al2O3(−Cs) Catalysts During Activation in H2 using in situ XRD and XRD‐CT
Authors: Farmer Daniela M. , Jacques Simon D. M. , Waller David , Boullosa Eiras Sara , Roy Kanak , Held Georg , Sankar Gopinathan , Beale Andrew M. 
Published: December 2022

AbstractInvited for this month's cover is the group of Andrew M. Beale at the University College London and at the Research Complex at Harwell (UK). The cover picture demonstrates how X‐rays can be used to obtain spatially resolved chemical imaging insight from within an industrial catalytic reactor. Understanding how the microstructure of the active Cu0 component in the commercially applicable Cu/ZnO/Al2O3(−Cs2O) low‐temperature water‐gas shift catalyst evolves under various H2 partial pressures in the presence/absence of a Cs promoter during thermal activation has been the subject of the present investigation. Read the full text of their Research Article at 10.1002/cmtd.202200015.
Outcomes of adjuvant radiation therapy after chemotherapy in resected extrahepatic cholangiocarcinoma: A National Cancer Database analysis
Authors: Shridhar Ravi , Blinn Paige , Huston Jamie , Meredith Kenneth L. 
Published: December 2022

AbstractBackgroundAdjuvant capecitabine is considered a standard of care for resected cholangiocarcinoma per the BILCAP trial. The role of adjuvant radiation therapy in that trial was not addressed. This study was designed to examine the outcomes of adjuvant radiation in patients who received chemotherapy for resected cholangiocarcinoma.MethodsUsing the National Cancer Database, the authors identified high‐risk patients with resected extrahepatic cholangiocarcinoma with either positive nodes (N+) or margins (R1) who received adjuvant chemotherapy between 2006 and 2014. Overall survival (OS) was determined with the Kaplan–Meier method. Propensity score matching (PSM) and multivariate analysis (MVA) were performed to identify prognostic factors for survival.ResultsThe authors identified 1478 patients after PSM who were included in the analysis. There was no difference in OS between patients receiving single‐agent chemotherapy and patients receiving multiagent chemotherapy (p = .69). There was a significant OS benefit associated with radiation therapy. The median OS and the 5‐year OS rate for radiated patients versus nonradiated patients were 34 months and 33% versus 27 months and 24% (p < .001) for the whole group, 30 months and 29% versus 24 months and 19% (p = .007) for the N+ subgroup, and 25 months and 23% versus 20 months and 12% (p = .03) for the R1 subgroup. MVA demonstrated that age, N stage, T stage, R1, and grade were associated with increased mortality, whereas adjuvant radiation was associated with decreased mortality (p < .001).ConclusionsThis is the first study showing that adjuvant radiation therapy after chemotherapy resulted in a significant OS benefit for patients with resected high‐risk extrahepatic cholangiocarcinoma. Trials are needed to address the role of radiation therapy in this population.
Global sensitivity analysis based on Gaussian‐process metamodelling for complex biomechanical problems
Authors: Wirthl Barbara , Brandstaeter Sebastian , Nitzler Jonas , Schrefler Bernhard A. , Wall Wolfgang A. 
Published: December 2022

AbstractBiomechanical models often need to describe very complex systems, organs or diseases, and hence also include a large number of parameters. One of the attractive features of physics‐based models is that in those models (most) parameters have a clear physical meaning. Nevertheless, the determination of these parameters is often very elaborate and costly and shows a large scatter within the population. Hence, it is essential to identify the most important parameters (worth the effort) for a particular problem at hand. In order to distinguish parameters which have a significant influence on a specific model output from non‐influential parameters, we use sensitivity analysis, in particular the Sobol method as a global variance‐based method. However, the Sobol method requires a large number of model evaluations, which is prohibitive for computationally expensive models. We therefore employ Gaussian processes as a metamodel for the underlying full model. Metamodelling introduces further uncertainty, which we also quantify. We demonstrate the approach by applying it to two different problems: nanoparticle‐mediated drug delivery in a complex, multiphase tumour‐growth model, and arterial growth and remodelling. Even relatively small numbers of evaluations of the full model suffice to identify the influential parameters in both cases and to separate them from non‐influential parameters. The approach also allows the quantification of higher‐order interaction effects. We thus show that a variance‐based global sensitivity analysis is feasible for complex, computationally expensive biomechanical models. Different aspects of sensitivity analysis are covered including a transparent declaration of the uncertainties involved in the estimation process. Such a global sensitivity analysis not only helps to massively reduce costs for experimental determination of parameters but is also highly beneficial for inverse analysis of such complex models.
Postharvest commercial quality improvement of broccoli (Brassica oleracea L.) after foliar biofortification with selenium
Authors: Trod Betiana S. , Buttarelli Melina S. , Stoffel María M. , Céccoli Gabriel , Olivella Laura , Barengo Pamela B. , Llugany Mercè , Guevara María G. , Muñoz Fernando F. , Daurelio Lucas D. 
Published: December 2022

AbstractBroccoli heads (Brassica oleracea L. var. italica Plenck) are one of the most valuable vegetable crops parts due to their high nutritional content. Nevertheless, they are quickly decomposed during postharvest, with the consequent loss of commercial quality. Several postharvest treatments have been analyzed to delay this process, but the influence of preharvest treatments has been little studied. Previously, we have examined the effect of foliar application of sodium selenate and selenite (50 and 100 g ha−1) on selenium accumulation and commercial quality of broccoli at harvest time. Here, we analyze the effect of these preharvest treatments on postharvest shelf‐life of broccoli grown under field and pot conditions, in “Belstar” and “Legend” cultivars. Examination of the external appearance of the heads, quantified by Hue and L parameters, showed a marked delay in yellowing in treated samples compared to controls. In addition, selenium treatments mostly maintained the firmness and diameter of the heads. Similar results were obtained for both growing conditions and cultivars. Noticeably, the nutritional composition of broccoli heads was maintained or even improved after several foliar treatments with selenium compared with the control ones. Finally, the same analysis was performed in broccoli heads stored at low temperature, also showing different parameters improvement in the selenium‐treated groups compared to control. So, we can conclude that foliar selenium treatment, besides being an overcoming strategy to obtain selenium‐enriched broccoli, also contributes to improving the quality of heads during postharvest.
Oxidation of N‐Alkyl(iso)quinolinium Salts Over TEMPO@Metal‐Organic Framework Heterogeneous Photocatalyst†
Authors: Fu Shan‐Shan , Yuan Qiang‐Qiang , Ma Lihua , Zhang Zhi‐Ming , Lu Tong‐Bu , Guo Song 
Published: December 2022

AbstractQuinolones and isoquinolones are of particular importance to pharmaceutical industry due to their diverse biological activities. However, their synthetic protocols were limited by high toxicity, high energy consumption, poor functional group tolerance and noble metal catalyst. This study concerns the development of a series of TEMPO@PCN‐222 (TEMPO: 2,2,6,6‐tetramethylpiperidinyl‐1‐oxy; PCN: porous coordination network) composite photocatalysts by coordinating different amount of 4‐carboxy‐TEMPO with the secondary building units of PCN‐222. Upon visible‐light irradiation, photogenerated holes in the highest occupied molecular orbital of PCN‐222 can smoothly transfer to TEMPO, which can significantly boost the photosynthesis of bioactive (iso)quinolones from readily available N‐alkyl(iso)quinolinium salts. TEMPO@PCN‐222 exhibits an outstanding catalytic stability and substrate tolerance with a 1‐methyl‐2‐quinolinone yield of 86.7 %, over four times that with PCN‐222 (21.4 %). This work provides a new route to construct composite photocatalysts from abundant starting materials for efficient photosynthesis of high value‐added chemicals.
Tailup expression in Drosophila larval and adult cardiac valve cells
Authors: Meyer Christian , Bataillé Laetitia , Drechsler Maik , Paululat Achim 
Published: December 2022

SummaryIn Drosophila larvae, the direction of blood flow within the heart tube, as well as the diastolic filling of the posterior heart chamber, is regulated by a single cardiac valve. This valve is sufficient to close the heart tube at the junction of the ventricle and the aorta and is formed by only two cells; both are integral parts of the heart tube. The valve cells regulate hemolymph flow by oscillating between a spherical and a flattened cell shape during heartbeats. At the spherical stage, the opposing valve cells close the heart lumen. The dynamic cell shape changes of valve cells are supported by a dense, criss‐cross orientation of myofibrils and the presence of the valvosomal compartment, a large intracellular cavity. Both structures are essential for the valve cells' function. In a screen for factors specifically expressed in cardiac valve cells, we identified the transcription factor Tailup. Knockdown of tailup causes abnormal orientation and differentiation of cardiac muscle fibers in the larval aorta and inhibits the formation of the ventral longitudinal muscle layer located underneath the heart tube in the adult fly and affects myofibrillar orientation of valve cells. Furthermore, we have identified regulatory sequences of tup that control the expression of tailup in the larval and adult valve cells.
Plant phenological dataset collated by the Finnish Society of Sciences and Letters
Authors: Holopainen Jari , Helama Samuli , Väre Henry 
Published: December 2022

AbstractLong records of phenological observations constitute data for ecological, climate, and global change studies. Here we provide a unique dataset of plant phenological observations made in boreal Europe between 1750 and 1965 from locations situated across historical and modern Finland, mostly between 70° and 60° N and 30° and 20° E. This dataset was generated initially by the efforts of several generations of volunteers representing naturalists whose field observations and notes had initially made the continuous collection of the data possible. In addition, the data were collated by the Finnish Economic Society and the Finnish Society of Sciences and Letters and published irregularly in the form of several monographs and periodicals by contemporary academic enthusiasts. Each phenological observation contains 11 features including: site name, site latitude, site longitude, scientific species name, phenological stage, and (if any) its substage, year, date (month and day) and the day since the summer solstice, the original literature source, and outlier estimate. Species names given originally either in Latin, Finnish, German, and/or Swedish were transformed into scientific species names. Moreover, outdated taxonomic names were updated as appropriate. Phenological stages that had been given originally either in German, Finnish, and/or Swedish were transformed into English and standardized by excluding synonyms. Site names were adopted at the county level, with corresponding latitude and longitude generated herein. The digitized dataset represents 265,478 observations of 985 taxa (assigned to variety/subspecies/species/hybrid/genus) for their 16 different phenological stages made in 371 locations across the region. We provide this dataset to support comparative studies and modeling projects, seeking to improve the understanding of terrestrial ecosystem dynamics and its responses to a changing environment from a local to a global scale. Use of this dataset for academic or educational purposes is encouraged as long as the data source is correctly cited with attribution given to this presentation of the data. Users are free to use and analyze the data; additionally, we would like to hear from other researchers who use this dataset in teaching or for their own research.
Comparing the test–retest reliability of resting‐state functional magnetic resonance imaging metrics across single band and multiband acquisitions in the context of healthy aging
Authors: Cahart Marie‐Stephanie , O'Daly Owen , Giampietro Vincent , Timmers Maarten , Streffer Johannes , Einstein Steven , Zelaya Fernando , Dell'Acqua Flavio , Williams Steven C. R. 
Published: December 2022

AbstractThe identification of meaningful functional magnetic resonance imaging (fMRI) biomarkers requires measures that reliably capture brain performance across different subjects and over multiple scanning sessions. Recent developments in fMRI acquisition, such as the introduction of multiband (MB) protocols and in‐plane acceleration, allow for increased scanning speed and improved temporal resolution. However, they may also lead to reduced temporal signal to noise ratio and increased signal leakage between simultaneously excited slices. These methods have been adopted in several scanning modalities including diffusion weighted imaging and fMRI. To our knowledge, no study has formally compared the reliability of the same resting‐state fMRI (rs‐fMRI) metrics (amplitude of low‐frequency fluctuations; seed‐to‐voxel and region of interest [ROI]‐to‐ROI connectivity) across conventional single‐band fMRI and different MB acquisitions, with and without in‐plane acceleration, across three sessions. In this study, 24 healthy older adults were scanned over three visits, on weeks 0, 1, and 4, and, on each occasion, underwent a conventional single band rs‐fMRI scan and three different rs‐fMRI scans with MB factors 4 and 6, with and without in‐plane acceleration. Across all three rs‐fMRI metrics, the reliability scores were highest with MB factor 4 with no in‐plane acceleration for cortical areas and with conventional single band for subcortical areas. Recommendations for future research studies are discussed.
Midterm postoperative outcomes of different types of surgical reconstruction of sinus venosus atrial septal defects with anomalous pulmonary venous connection: The Results of Prospective Cohort Study
Authors: Bhende Vishal V. , Sharma Tanishq S. , Mehta Deepakkumar V. , Trivedi Bhadra Y. , Kumar Amit , Patel Viral B. , Panesar Gurpreet , Soni Kunal , Dhami Kartik B. , Patel Nirja , Pathan Sohilkhan R. , Majmudar Hardil P. 
Published: December 2022

AbstractBackground and AimsSinus venosus atrial septal defects (SVASDs) constitute a substantial part of atrial septal defects and are usually characterized by anomalous pulmonary venous connection (APVC), causing complications like sinus node dysfunction and arrhythmias. Several surgical approaches are used for treating SVASDs in pediatric patients, including single‐ and two‐patch techniques. The study aimed to prospectively evaluate and compare the safety and efficacy of these two methods with different follow‐up periods.MethodsTen patients aged 1–8 years with SVASDs and partial APVC were enrolled in the study at Bhanubhai and Madhuben Patel Cardiac Centre, Karamsad, India, between December 2018 and October 2021. The single‐patch (sandwich‐patch) technique was used in two patients, whereas the two‐patch (dual‐patch) technique with autologous pericardium was used in seven. Safety was assessed as the absence of complications in the follow‐up periods of 6 months, 1, and 2 years, whereas efficacy was estimated by the preserved sinus rhythm and the development of arrhythmias. Electrocardiographic and echocardiographic methods were used to evaluate both parameters.ResultsNo deaths, reoperations, pulmonary vein, and superior vena cava (SVC) stenosis or phrenic nerve palsy were observed among the 10 patients in the three follow‐up periods. Sinus rhythm was arrested in two of the seven patients who underwent two‐patch repair, whereas no rhythm disturbances occurred in those who underwent single‐patch repair.ConclusionBoth techniques used in SVASD repair with autologous pericardium proved to cause the smaller rate of complications in midterm postsurgical phase. However, there is a potentially great risk of the development of sinus node malfunction after the application of the two‐patch technique. Therefore, methods avoiding sinus node interference are preferred in patients with partial APVC involving SVC.
Animal models of chemotherapy‐induced peripheral neuropathy for hematological malignancies: A review
Authors: Lv Xiaoli , Mao Yingwei , Cao Song , Feng Yonghuai 
Published: December 2022

AbstractChemotherapy is one of the main treatments for hematologic malignancies. However, chemotherapy‐induced peripheral neuropathy (CIPN) is one of the most common long‐term toxic reactions in chemotherapy, and the occurrence of CIPN affects patients’ quality of life and can cause interruption of chemotherapy in severe cases, thus reducing the efficacy of chemotherapy. We currently summarize the existing CIPN animal models, including the characteristics of several common animal models such as bortezomib‐induced peripheral neuropathy, vincristine‐induced peripheral neuropathy, and oxaliplatin‐induced peripheral neuropathy. It was found that CIPN may lead to behavioral, histopathological, and neurophysiological changes inducing peripheral neuropathy. However, the mechanism of CIPN has not been fully elucidated, especially the prevention and treatment protocols need to be improved. Therefore, this review article summarizes the progress of research on CIPN animal models and the possible mechanisms and treatment of CIPN.
Evaluation of the Pharmacokinetics of Dapagliflozin in Patients With Chronic Kidney Disease With or Without Type 2 Diabetes Mellitus
Authors: Penland Robert C. , Melin Johanna , Boulton David W. , Tang Weifeng 
Published: December 2022

AbstractEvidence shows that sodium‐glucose cotransporter 2 inhibitors, such as dapagliflozin, can delay the progressive decline of kidney function in patients with type 2 diabetes mellitus (T2DM) and chronic kidney disease (CKD). We used a population pharmacokinetics (popPK) model to characterize the pharmacokinetics of dapagliflozin in patients with CKD and compare dapagliflozin systemic exposure in different populations, such as CKD with or without T2DM and T2DM without CKD. A 2‐compartmental popPK model was developed from a previous popPK model. The final popPK model was based on 9715 dapagliflozin plasma concentrations from 3055 patients included in clinical studies involving adults with CKD with or without T2DM, adults with T2DM, healthy subjects, and pediatric patients with T2DM. Overall, the apparent clearance for patients treated with dapagliflozin was 21.6 L/h, similar to previous estimates in adults with T2DM and healthy subjects (22.9 L/h). Model‐derived area under the plasma concentration–time curve (AUC) was not meaningfully different between patients with CKD with and without T2DM. Median AUC was 1.6‐fold higher in adult patients with CKD with T2DM compared with adult patients with T2DM without CKD. Compared with patients with normal kidney function (estimated glomerular filtration rate ≥90 mL/min/1.73 m2), median AUC was 2.4‐fold higher in patients with CKD (with/without T2DM) with estimated glomerular filtration rate 15–29 mL/min/1.73 m2 owing to decreased renal clearance of dapagliflozin. A higher AUC was observed in patients with a higher age or lower body weight but was not considered clinically relevant. This popPK model adequately described dapagliflozin pharmacokinetics and found that systemic exposure in patients with CKD was consistent, irrespective of T2DM status.
Cardiac magnetic resonance findings in acute and post‐acute COVID‐19 patients with suspected myocarditis
Authors: Palmisano Anna , Vignale Davide , Bruno Elisa , Peretto Giovanni , De Luca Giacomo , Campochiaro Corrado , Tomelleri Alessandro , Agricola Eustachio , Montorfano Matteo , Esposito Antonio 
Published: December 2022

AbstractIntroductionCardiac injury is commonly reported in COVID‐19 patients, resulting associated to pre‐existing cardiovascular disease, disease severity, and unfavorable outcome. Aim is to report cardiac magnetic resonance (CMR) findings in patients with myocarditis‐like syndrome during the acute phase of SARS‐CoV‐2 infection (AMCovS) and post‐acute phase (cPACS).MethodsBetween September 2020 and January 2022, 39 consecutive patients (24 males, 58%) were referred to our department to perform a CMR for the suspicion of myocarditis related to AMCovS (n = 17) and cPACS (n = 22) at multimodality evaluation (clinical, laboratory, ECG, and echocardiography).CMR was performed for the assessment of volume, function, edema and fibrosis with standard sequences and mapping techniques. CMR diagnosis and the extension and amount of CMR alterations were recorded.ResultsPatients with suspected myocarditis in acute and post‐COVID settings were mainly men (10 (59%) and 12 (54.5%), respectively) with older age in AMCovS (58 [48–64]) compared to cPACS (38 [26–53]). Myocarditis was confirmed by CMR in most of cases: 53% of AMCovS and 50% of cPACS with negligible LGE burden (3 [IQR, 1–5] % and 2 [IQR, 1–4] %, respectively).Myocardial infarction was identified in 4/17 (24%) patients with AMCovS. Cardiomyopathies were identified in 12% (3/17) and 27% (6/22) of patients with AMCovS and cPACS, including DCM, HCM and mitral valve prolapse.ConclusionsIn patients with acute and post‐acute COVID‐19 related suspected myocarditis, CMR improves diagnostic accuracy characterizing ischemic and non‐ischemic injury and unraveling subclinical cardiomyopathies.
Distinct non‐coding RNA cargo of extracellular vesicles from M1 and M2 human primary macrophages
Authors: Pantazi Paschalia , Clements Toby , Venø Morten , Abrahams Vikki M. , Holder Beth 
Published: December 2022

AbstractMacrophages are important antigen presenting cells which can release extracellular vesicles (EVs) carrying functional cargo including non‐coding RNAs. Macrophages can be broadly classified into M1 ‘classical’ and M2 ‘alternatively‐activated’ macrophages. M1 macrophages have been linked with inflammation‐associated pathologies, whereas a switch towards an M2 phenotype indicates resolution of inflammation and tissue regeneration. Here, we provide the first comprehensive analysis of the small RNA cargo of EVs from human M1 and M2 primary macrophages. Using small RNA sequencing, we identified several types of small non‐coding RNAs in M1 and M2 macrophage EVs including miRNAs, isomiRs, tRNA fragments, piRNA, snRNA, snoRNA and Y‐RNA fragments. Distinct differences were observed between M1 and M2 EVs, with higher relative abundance of miRNAs, and lower abundance of tRNA fragments in M1 compared to M2 EVs. MicroRNA‐target enrichment analysis identified several gene targets involved in gene expression and inflammatory signalling pathways. EVs were also enriched in tRNA fragments, primarily originating from the 5′ end or the internal region of the full length tRNAs, many of which were differentially abundant in M1 and M2 EVs. Similarly, several other small non‐coding RNAs, namely snRNAs, snoRNAs and Y‐RNA fragments, were differentially enriched in M1 and M2 EVs; we discuss their putative roles in macrophage EVs. In conclusion, we show that M1 and M2 macrophages release EVs with distinct RNA cargo, which has the potential to contribute to the unique effect of these cell subsets on their microenvironment.