bluuebunny/crossref_metadata_2025_split huggingface dataset:
LRP5-/6 gene polymorphisms and its association with risk of abnormal bone mass in postmenopausal women
Authors: Li Jun , Liu Zebing , Ren Yanxia , Shao Han , Li Siyuan 
Published: May 2023

Abstract
Objectives
To assess LRP5-/6 gene polymorphisms and its association with risk of abnormal bone mass (ABM) in postmenopausal women.

Methods
The study recruited 166 patients with ABM (case group) and 106 patients with normal bone mass (control group) based on bone mineral density (BMD) results. Multi-factor dimensionality reduction (MDR) was used to analyze the interaction between the Low-density lipoprotein receptor-related protein 5 (LRP5) gene (rs41494349, rs2306862) and the Low-density lipoprotein receptor-related protein 6 (LRP6) gene (rs10743980, rs2302685) and the subjects’ clinical characteristics of age and menopausal years.

Results
(1) Logistic regression analysis showed that the subjects with the CT or TT genotype at rs2306862 had a higher risk of ABM than those with the CC genotype (OR = 2.353, 95%CI = 1.039–6.186; OR = 2.434, 95%CI = 1.071, 5.531; P < 0.05). The subjects with the TC genotype at rs2302685 had a higher risk of ABM than those with the TT genotype (OR = 2.951, 95%CI = 1.030–8.457, P < 0.05). (2) When taking the three Single-nucleotide polymorphisms (SNPs) together, the accuracy was the highest with the cross-validation consistency of 10/10 (OR = 1.504, 95%CI:1.092–2.073, P < 0.05), indicating that the LRP5 rs41494349 and LRP6 rs10743980, rs2302685 were interactively associated with the risk of ABM. (3) Linkage disequilibrium (LD) results revealed that the LRP5 (rs41494349,rs2306862) were in strong LD (D′ > 0.9, r2 > 0.3). AC and AT haplotypes were significantly more frequently distributed in the ABM group than in the control group, indicating that subjects carrying the AC and AT haplotypes were associated with an increased risk of ABM (P < 0.01). (4) MDR showed that rs41494349 & rs2302685 & rs10743980 & age were the best model for ABM prediction. The risk of ABM in “high-risk combination” was 1.00 times that of “low-risk combination”(OR = 1.005, 95%CI: 1.002–1.008, P < 0.05). (5) MDR showed that there was no significant association between any of the SNPs and menopausal years and ABM susceptibility.

Conclusion
These findings indicate that LRP5-rs2306862 and LRP6-rs2302685 polymorphisms and gene–gene and gene–age interactions may increase the risk of ABM in postmenopausal women. There was no significant association between any of the SNPs and menopausal years and ABM susceptibility.
Profile and outcome of patients with Warfarin Toxicity admitted in a tertiary care hospital in Bhutan: a cross-sectional study investigators and institutions
Authors: Penjore Yeshey , Dorji Thinley , Dorji Sangay , Tamang Saran Tenzin 
Published: May 2023

Abstract
Objective
To study the profile, clinical presentation and outcome of hospital stay among patients admitted with warfarin toxicity at the Jigme Dorji Wangchuck National Referral Hospital, Bhutan. This was a cross-sectional study with a review of hospital records of patients admitted between 01 and 2018 and 30 June 2020.

Results
There were 22 admissions due to warfarin toxicity. The mean age of patients was 55.9 (± SD 20.2) years, the median duration of warfarin therapy was 30 months (IQR 4.8, 69 months). The indications for warfarin were atrial fibrillation (9, 40.9%), mechanical heart valves (6, 27.3%), deep vein thrombosis (6, 27.3%) and pulmonary thromboembolism (1, 4.5%). The mean of dosage of warfarin was 4.3 (± 2.6) mg and the cumulative dosage in the week prior to admission was 30.9 (± 18.6) mg. The mean of INR at presentation was 7.7 (± 4.3) with the maximum noted at 20. The patients presented with gastrointestinal bleeding, muscle haematomas, epistaxis and oral cavity bleeding. There was no mortality related to warfarin toxicity. The reasons for warfarin toxicity included patient dosing error and drug interactions. Warfarin therapy requires appropriate patient education, adequate facilities for follow-up and avoidance of warfarin wherever possible in clinical settings.
Molecular epidemiology of nontuberculous mycobacteria isolated from tuberculosis-suspected patients
Authors: Tarashi Samira , Sakhaee Fatemeh , Masoumi Morteza , Ghazanfari Jajin Morteza , Siadat Seyed Davar , Fateh Abolfazl 
Published: May 2023

AbstractIt is a growing problem around the world to deal with nontuberculous mycobacteria infection (NTM), but its clinical significance is still largely unknown. This study aims to investigate the epidemiology of NTM infections from various clinical samples and determine their clinical significance. From December 2020 to December 2021, 6125 clinical samples were collected. In addition to phenotypic detection, genotypic detection through multilocus sequence typing (hsp65, rpoB, and 16S rDNA genes) and sequencing was also conducted. Records of patients were consulted for clinical information, such as symptoms and radiological findings. Of the 6,125 patients, 351 (5.7%) were positive for acid-fast bacteria (AFB). Out of 351 AFB, 289 (82.3%) and 62 (17.7%) subjects were identified as M. tuberculosis complex (MTC) and NTM strains, respectively. Isolates of Mycobacterium simiae and M. fortuitum were the most frequent, followed by isolates of M. kansasii and M. marinum. We also isolated M. chelonae, M. canariasense, and M. jacuzzii, which are rarely reported. Symptoms (P = 0.048), radiographic findings (P = 0.013), and gender (P = 0.039) were associated with NTM isolates. M. Fortuitum, M. simiae, and M. kansasii presented with bronchiectasis, infiltration, and cavitary lesions most frequently, while cough was the most common symptom. In conclusion, Mycobacterium simiae and M. fortuitum were presented in seventeen and twelve NTM isolates from the collected samples. There is evidence that NTM infections in endemic settings may contribute to the dissemination of various diseases and the control of tuberculosis. In spite of this, further research is needed to evaluate the clinical significance of NTM isolates.
Are COVID-19 systematic reviews up to date and can we tell? A cross-sectional study
Authors: McDonald Steve , Turner Simon L. , Nguyen Phi-Yen , Page Matthew J. , Turner Tari 
Published: May 2023

Abstract
Background
COVID-19 led to a rapid acceleration in the number of systematic reviews. Readers need to know how up to date evidence is when selecting reviews to inform decisions. This cross-sectional study aimed to evaluate how easily the currency of COVID-19 systematic reviews published early in the pandemic could be determined and how up to date these reviews were at the time of publication.

Methods
We searched for systematic reviews and meta-analyses relevant to COVID-19 added to PubMed in July 2020 and January 2021, including any that were first published as preprints. We extracted data on the date of search, number of included studies, and date first published online. For the search date, we noted the format of the date and where in the review this was reported. A sample of non-COVID-19 systematic reviews from November 2020 served as a comparator.

Results
We identified 246 systematic reviews on COVID-19. In the abstract of these reviews, just over half (57%) reported the search date (day/month/year or month/year) while 43% failed to report any date. When the full text was considered, the search date was missing from 6% of reviews. The median time from last search to publication online was 91 days (IQR 63–130). Time from search to publication was similar for the subset of 15 rapid or living reviews (92 days) but shorter for the 29 reviews published as preprints (37 days). The median number of studies or publications included per review was 23 (IQR 12–40). In the sample of 290 non-COVID SRs, around two-thirds (65%) reported the search date while a third (34%) did not include any date in the abstract. The median time from search to publication online was 253 days (IQR 153–381) and each review included a median of 12 studies (IQR 8–21).

Conclusions
Despite the context of the pandemic and the need to easily ascertain the currency of systematic reviews, reporting of the search date information for COVID-19 reviews was inadequate. Adherence to reporting guidelines would improve the transparency and usefulness of systematic reviews to users.
What acceleration data from wildlife collars and animal body mass tell us about seed dispersal
Authors: Buchmann Carsten M. , Dreyling Lukas , Constantin Mihaela , Schurr Frank M. 
Published: May 2023

Abstract
Background
The seeds of many plant species can be dispersed over long distances in animal fur (epizoochory). Quantifying epizoochory in the wild is, however, challenging, since it is difficult to measure the retention times of seeds in fur. These retention times depend on the acceleration that seeds experience and that can detach seeds from fur. Wildlife collars containing accelerometers may thus provide crucial information on epizoochorous seed dispersal. However, this is only the case if acceleration of the animal’s neck (where collars are attached) is informative of acceleration of the animal’s main body (where most seeds are transported).

Methods
We used accelerometers to simultaneously measure acceleration at the neck, breast and the upper hind leg of 40 individuals of eight mammal species spanning a large range of body masses (26–867 kg). We then quantified maximum acceleration as the 95%-quantile of the resultant acceleration (of all measured values in data intervals of 5 s).

Results
Maximum acceleration was comparable between the neck and breast but substantially higher at the hind leg. Maximum acceleration measured by neck collars and body mass jointly explained 81% of the variance in maximum acceleration of the breast and 62% of the variance in maximum acceleration of the leg.

Conclusions
Acceleration measured by neck collars is informative of the acceleration experienced by seeds attached to other body parts (breast and leg). When combined with animal movement data and lab measurements of how fur acceleration affects seed release and retention times, widely used collar accelerometers can thus be used to assess distances of epizoochorous seed dispersal.
Selenide-linked polydopamine-reinforced hybrid hydrogels with on-demand degradation and light-triggered nanozyme release for diabetic wound healing
Authors: Li Wenjing , Bei Ying , Pan Xiangqiang , Zhu Jian , Zhang Zhengbiao , Zhang Tinglin , Liu Jieting , Wu Dan , Li Meng , Wu Yan , Gao Jie 
Published: May 2023

Abstract

Background
Multifunctional hydrogels with controllable degradation and drug release have attracted extensive attention in diabetic wound healing. This study focused on the acceleration of diabetic wound healing with selenide-linked polydopamine-reinforced hybrid hydrogels with on-demand degradation and light-triggered nanozyme release.

Methods

Herein, selenium-containing hybrid hydrogels, defined as DSeP@PB, were fabricated
via
the reinforcement of selenol-end capping polyethylene glycol (PEG) hydrogels by polydopamine nanoparticles (PDANPs) and Prussian blue nanozymes in a one-pot approach in the absence of any other chemical additive or organic solvent based on diselenide and selenide bonding-guided crosslinking, making them accessible for large-scale mass production.

Results
Reinforcement by PDANPs greatly increases the mechanical properties of the hydrogels, realizing excellent injectability and flexible mechanical properties for DSeP@PB. Dynamic diselenide introduction endowed the hydrogels with on-demand degradation under reducing or oxidizing conditions and light-triggered nanozyme release. The bioactivity of Prussian blue nanozymes afforded the hydrogels with efficient antibacterial, ROS-scavenging and immunomodulatory effects, which protected cells from oxidative damage and reduced inflammation. Further animal studies indicated that DSeP@PB under red light irradiation showed the most efficient wound healing activity by stimulating angiogenesis and collagen deposition and inhibiting inflammation.

Conclusion
The combined merits of DSeP@PB (on-demand degradation, light-triggered release, flexible mechanical robustness, antibacterial, ROS-scavenging and immunomodulatory capacities) enable its high potential as a new hydrogel dressing that can be harnessed for safe and efficient therapeutics for diabetic wound healing.
Effervescent cannabidiol solid dispersion-doped dissolving microneedles for boosted melanoma therapy via the “TRPV1-NFATc1-ATF3” pathway and tumor microenvironment engineering
Authors: Shi Jiachen , Ma Qiuling , Su Wenting , Liu Congyan , Zhang Huangqin , Liu Yuping , Li Xiaoqi , Jiang Xi , Ge Chang , Kong Fei , Chen Yan , Qu Ding 
Published: May 2023

Abstract

Background
Conventional dissolving microneedles (DMNs) face significant challenges in anti-melanoma therapy due to the lack of active thrust to achieve efficient transdermal drug delivery and intra-tumoral penetration.

Methods

In this study, the effervescent cannabidiol solid dispersion-doped dissolving microneedles (Ef/CBD-SD@DMNs) composed of the combined effervescent components (CaCO
3
& NaHCO
3
) and CBD-based solid dispersion (CBD-SD) were facilely fabricated by the “one-step micro-molding” method for boosted transdermal and tumoral delivery of cannabidiol (CBD).

Results

Upon pressing into the skin, Ef/CBD-SD@DMNs rapidly produce CO
2
bubbles through proton elimination, significantly enhancing the skin permeation and tumoral penetration of CBD. Once reaching the tumors, Ef/CBD-SD@DMNs can activate transient receptor potential vanilloid 1 (TRPV1) to increase Ca
2+
influx and inhibit the downstream NFATc1-ATF3 signal to induce cell apoptosis. Additionally, Ef/CBD-SD@DMNs raise intra-tumoral pH environment to trigger the engineering of the tumor microenvironment (TME), including the M1 polarization of tumor-associated macrophages (TAMs) and increase of T cells infiltration. The introduction of Ca
2+
can not only amplify the effervescent effect but also provide sufficient Ca
2+
with CBD to potentiate the anti-melanoma efficacy. Such a “one stone, two birds” strategy combines the advantages of effervescent effects on transdermal delivery and TME regulation, creating favorable therapeutic conditions for CBD to obtain stronger inhibition of melanoma growth in vitro and in vivo.

Conclusions
This study holds promising potential in the transdermal delivery of CBD for melanoma therapy and offers a facile tool for transdermal therapies of skin tumors.
The safety of lookalikes: a new THC beverage enhancer and a non-THC counterpart
Authors: Brown Geoffrey W. , DeGelorm Anthony , Bellnier Terrance J. 
Published: May 2023

AbstractA new tetrahydrocannabinol (THC) beverage enhancer is available to medical and recreational cannabis consumers across the US. Beverage enhancers that do not contain THC, but instead contain flavored concentrates and/or other additives such as caffeine, are used by squirting the contents of a bottle into water, or other beverage of choice, ad libitum and can be used in a titratable manner according to the user’s preference or taste. The THC beverage enhancer described herein has an important safety feature: a mechanism that allows users to measure out a 5-mg dose of THC before they add it to their beverage. This mechanism, however, can be easily bypassed if a user attempts to use the product exactly the same way that its non-THC counterparts are used, by turning the bottle upside down and squirting the contents of the bottle into a beverage ad libitum. The THC beverage enhancer described herein would benefit from additional safety features such as a mechanism that prevents the contents of the bottle from leaving the device when turned upside down and a THC warning label.
Peritoneal deposits: PET/CT the keen observer
Authors: Mikawy Nora Nabil Abdou Ahmed , Elnaggar Heba T-allah Mohammed Yousry 
Published: May 2023

Abstract
Background
The peritoneum is the largest serous membrane in the body, it is a preferred site for metastasis of primary malignancies like mesothelioma. Accurate monitoring of tumor response to chemotherapy and detection of tumor recurrence is critical in management planning. CT is the gold standard for the detection of peritoneal deposits. PET scans are performed on patients with a variety of malignancies.

Results
The study included 70 patients, 36 females, and 34 males. The age ranged from 5 to 76 years. 59 patients presented to the PET CT unit with known 1ry tumor while 11 patients presented with metastasis of unknown 1ry. Lymphoma followed by colon were the commonest sites for the primary tumor with 15 and 13 patients for each respectively. Breast came in the 3rd place followed by endometrial carcinoma with 12 and 6 patients respectively. Bronchogenic carcinoma came after with 4 patients. Then melanoma, cervical, ovarian, and NET with 2 patients for each. One case had HCC. After performing the PET CT scan we detect the primary neoplastic process for 8 patients out of the 11 previously unknown cases and 3 cases being the unknown origin. These 8 patients were found to be 3 cases with 1ry breast lesions, 2 cases with ovarian masses, and the last 3 cases were colonic, HCC, and lymphoma. 60 of the patients included had peritoneal deposits while 10 were free. Ten patients were reviewed, finding 8 patients were free on CT and PET images, while 2 patients had avid spots. These spots were reviewed turning to be normal bowel activity. Visibility of the peritoneal deposits on CT images was observed, 33 patients had deposits that were not visualized on CT, and 27 were visible. The avid peritoneal deposits were observed. Fifty-eight patients had avid deposits while two patients had non-avid deposits. Fifty-five patients had high-grade avidity deposits while 3 patients had low-grade avidity deposits.

Conclusions
Evaluation of peritoneal malignancies with PET/CT is rewarding adding substantially to CT scanning alone.
Synthesis of N-Unprotected Diaryl Ketimines and Alkyl Ketimines from Ketones and Ammonia Using Porous Solid Acids with Analysis of Their Adsorption Behavior
Authors: Shibata Shintaro , Masui Yoichi , Narukawa Naoki , Shiroshita Takuya , Miya Haruhiko , Sato Rintaro , Tokutake Shunta , Tanaka Yoshiki , Onaka Makoto 
Published: May 2023

Abstract
The most atom-efficient synthetic method for N-unprotected ketimines (N-H ketimines) from ketones is the dehydration condensation reaction with ammonia (NH3). However, until now, few synthetic methods for N-H ketimines with high versatility have been known. In this study, we examined various solid acids and found that N-H diaryl ketimines with various functional groups on the aryl groups could be synthesized in high yields from diaryl ketones and NH3 under solvent-free conditions using silica-alumina (SiO2-Al2O3) or proton-exchanged Y-type zeolite (H-Y). Solid-state 13C and 15N NMR measurements indicated that the N-H ketimine formed in the pores of acidic zeolite was coordinated to NH4+ species on the pore surface. By quantum chemical calculations we also discussed the reason why the dehydration-condensation reaction between ketone and NH3, which is an endothermic reaction in a vacuum, was biased toward the product side in the actual experiment. In addition, this synthetic method can be applied to synthesize N-H alkyl ketimines with α-acidic hydrogens, which are less stable and more sensitive to hydrolysis and oligomerization than diaryl ketimines.
SERVICE LEARNING IN PREPARING STUDENTS TO ENSURE THE PSYCHOLOGICAL SAFETY OF SCHOOLCHILDREN
Authors: Demchenko Natalya A. , Shmeleva Elena A. , Kislyakov Pavel A. 
Published: May 2023

Relevance. The article substantiates the relevance of the search for practice-oriented technologies in the training of university students. The subject of the research is service learning technology, which combines academic training and practical activities of future teachers in specific situations in the interests of people in need of support. The purpose of the article is to substantiate the potential of service learning technology in preparing students to ensure the psychological safety of schoolchildren with limited health opportunities.
Research methods: literature analysis on the research problem, data systematization, survey, qualitative and quantitative data analysis, including percentage analysis. Study participants: students of T.G. Shevchenko Pridnestrovian State University (Republic of Moldova).
The results of the study. The awareness of students about the service learning method and their readiness to master it is revealed. The essence of the concept of service learning is revealed, the experience of its application in foreign and domestic universities is described, the possibility of its use in the work of preparing students to ensure the psychological safety of schoolchildren with disabilities is substantiated. The model of formation of students’ readiness to ensure the psychological safety of schoolchildren with disabilities is presented.
Conclusion. The practical significance of the research is expressed in the creation of a comfortable and safe educational environment declared for the priority purposes of the state. Service learning technology ensures the effective formation of professional competencies among students to ensure the psychological safety of children with disabilities and involves the use of trainings, games, exercises to relieve anxiety and aggressiveness of children, identifying threats and risks of psychological safety in real conditions of specific situations.