bluuebunny/crossref_metadata_2025_split huggingface dataset:
Precise Sizing and Collision Detection of Functional Nanoparticles by Deep Learning Empowered Plasmonic Microscopy
Authors: Wang Jingan , Sun Yi , Yang Yuting , Zhang Cheng , Zheng Weiqiang , Wang Chen , Zhang Wei , Zhou Lianqun , Yu Hui , Li Jinghong 
Published: January 2025

AbstractSingle nanoparticle analysis is crucial for various applications in biology, materials, and energy. However, precisely profiling and monitoring weakly scattering nanoparticles remains challenging. Here, it is demonstrated that deep learning‐empowered plasmonic microscopy (Deep‐SM) enables precise sizing and collision detection of functional chemical and biological nanoparticles. Image sequences are recorded by the state‐of‐the‐art plasmonic microscopy during single nanoparticle collision onto the sensor surface. Deep‐SM can enhance signal detection and suppresses noise by leveraging spatio‐temporal correlations of the unique signal and noise characteristics in plasmonic microscopy image sequences. Deep‐SM can provide significant scattering signal enhancement and noise reduction in dynamic imaging of biological nanoparticles as small as 10 nm, as well as the collision detection of metallic nanoparticle electrochemistry and quantum coupling with plasmonic microscopy. The high sensitivity and simplicity make this approach promising for routine use in nanoparticle analysis across diverse scientific fields.
Aging‐induced Alternation in the Gut Microbiota Impairs Host Antibacterial Defense
Authors: Gu Peng , Wei Rongjuan , Liu Ruofan , Yang Qin , He Yuxuan , Guan Jianbin , He Wenhao , Li Jiaxin , Zhao Yunfei , Xie Li , He Jie , Guo Qingling , Hu Jiajia , Bao Jingna , Wang Wandang , Guo Jiayin , Zeng Zhenhua , Chen Zhongqing , Jiang Yong , Liu Zhanguo , Chen Peng 
Published: January 2025

AbstractOlder individuals experience increased susceptibility and mortality to bacterial infections, but the underlying etiology remains unclear. Herein, it is shown that aging‐associated reduction of commensal Parabacteroides goldsteinii (P. goldsteinii) in both aged mice and humans critically contributes to worse outcomes of bacterial infection. The colonization of live P. goldsteinii conferred protection against aging‐associated bacterial infections. Metabolomic profiling reveals a protective compound, apigenin, generated by P. goldsteinii, antagonizes bacterial clearance defects in aged mice. AMP‐binding protein (ampB) is identified as a key gene involved in apigenin synthesis in P. goldsteinii using homologous recombination in bacteria. Mechanistically, apigenin binds directly to the potential sites on Fgr (M341 and D404), preventing its inhibitory role on Vav1 phosphorylation, and therefore promoting the activation of Cdc42/Rac1, Arp2/3 expression and subsequent actin reorganization, which contributes to the enhanced phagocytosis of macrophages to bacteria. Collectively, the findings suggest that dysbiosis of the gut microbiota may impair host defense mechanisms and increase susceptibility to bacterial infections in older adults and highlight the microbiota‐apigenin‐Fgr axis as a possible route to ameliorate aging‐associated antibacterial defects.
Economic Value and Determinants of Postharvest Losses of Tomato at the Retail Level: A Case Study in Kandy District, Sri Lanka
Authors: Thrikawala Sunil , Kandangama Nawarathna B. , Schwab Ben , Weerahewa Jeevika 
Published: January 2025

ABSTRACTThis study quantifies, assesses economic value, and evaluates determinants of losses of tomatoes at the retailer level. Data collected from randomly selected 122 retailers in the Kandy district of Sri Lanka was used for the analysis. On a typical day, a retailer purchases 24 kg of tomatoes at LKR 302 per kg and handles 30 kg. Daily losses account for 4 kg which accounts for 13% of the total volume handled and an economic loss of LKR 75 per kg. Female and educated retailers, as well as those adopting good practices such as discarding damaged tomatoes and using better packaging, experience lower losses and adoption of which is influenced by socioeconomic characteristics and business circumstances. Additionally, lower economic losses were observed among less experienced retailers handling smaller volumes in smaller stores with strong upstream connections. Incentivizing good handling practices among retailers with high losses is recommended.
Phase Level Assessment of Ergonomic Intervention Effectiveness in Reducing Knee Musculoskeletal Disorder Risks During Residential Roof Shingle Installation
Authors: Zerin Nazia , Breloff Scott P. , Dai Fei , Carey Robert E. , Warren Christopher M. , Moore Kevin D. , Hawke Ashley L. , Sinsel Erik W. , Wu John Z. 
Published: January 2025

ABSTRACTBackgroundThis study aimed to assess how knee savers (KSs) and knee pads (KPs) alleviate risks of knee musculoskeletal disorders (MSDs) among roofers during various phases of shingle installation. These phases encompass (1) reaching for shingles, (2) placing shingles, (3) grabbing a nail gun, (4) moving to the first nailing position, (5) nailing shingles, (6) replacing the nail gun, and (7) returning to an upright position.MethodsIn a laboratory setting, nine male participants simulated the shingle installation task on a slope‐adjustable roof platform (0°, 15°, and 30° slopes) under four intervention conditions: no intervention (NO); with KPs only (KP); with KSs only (KS); and with both KPs and KSs (BO). Knee flexion, abduction, adduction, and internal/external rotations were measured to assess intervention impact through statistical analysis.ResultsPhase 5 (nailing shingles), one of the riskiest phases, saw reduced knee rotations, with BO and KP interventions being the most effective. Phase 6 (replacing the nail gun) exhibited notable reductions in all knee rotations, primarily due to BO intervention. Significant improvements in certain knee angles for other phases were noted, particularly with BO intervention.ConclusionsBO and KP can lower knee strain by minimizing extreme knee postures and thereby reducing the risk of MSDs during the installation of shingles, especially at critical periods and on steeper slopes. This study highlights the importance of applying focused ergonomic techniques in the roofing sector to improve workers' musculoskeletal health.
Amino‐Acid‐Induced Circularly Polarized Luminescence of Octahedral Lanthanide Cage
Authors: Zhao Teng , Zhang Yu‐Fei , Wang Guang‐Hao , Wang Xuan‐Xuan , Feng Peng‐Fei , Zang Shuang‐Quan 
Published: January 2025

AbstractChiral metal organic cage compounds with excellent circularly polarized luminescent performance have broad application prospects in many fields. Herein, two lanthanide complexes with luminescent properties in the form of racemic hexagonal octahedral cages were synthesized using a tri (β‐diketone) ligand. Eu6(C21H6F15O6)8(H2O)6 exhibited red light emission with high quantum yields of 61 %. Then, we successfully synthesized lanthanide compounds with circularly polarized luminescent activity by chiral induction of the racemic mixture with arginine. The luminescence asymmetry factor was 0.53, and the circularly polarized luminescent merit figure reached 0.323. High‐performance circularly polarized luminescent of racemic cage like compound systems have achieved using cheap and readily available chiral amino acid molecules through chiral induction.
Discrete Hybrid Vanadium‐oxo Cluster as a Targeted Tool for Selective Protein Oxidative Modifications and Cleavage
Authors: Moussawi Mhamad Aly , de Azambuja Francisco , Parac‐Vogt Tatjana N. 
Published: January 2025

AbstractUnderstanding the impact of oxidative modification on protein structure and functions is essential for developing therapeutic strategies to combat macromolecular damage and cell death. However, selectively inducing oxidative modifications in proteins under physiological conditions remains challenging. Herein we demonstrate that [V6O13{(OCH2)3CCH2OH}2]2− (V6‐OH) hybrid metal‐oxo cluster can be used for selective protein oxidative cleavage and modifications. We present the first example of a protein‐bound hybrid vanadate cluster, where its interactions with protein surfaces and the redox activity of vanadium enable selective oxidative modifications. Single Crystal X‐ray Diffraction (SC‐XRD) of the V6‐OH and hen egg white lysozyme (HEWL) complex revealed that the binding is dictated both by the inorganic core and the organic ligands attached to it. Selective oxidation or cleavage of HEWL occurs under physiological conditions by producing reactive oxygen species (ROS) in presence of ascorbate (Asc) as a reducing agent. The outcome of the oxidative reaction can be tuned by varying the concentration of V6‐OH to result either in selective oxidation of the amino acid side chains or peptide bond cleavage. LC–MS and crystallography revealed that oxidative modifications were mainly concentrated near the cluster binding sites, providing spatial control of ROS production. This study advances our understanding of vanadium's role in biological systems and demonstrates the potential of hybrid metal‐oxo clusters in protein modification.
Trinuclear Cobalt/Nickel‐Based Metal–Organic Frameworks as Fluorescent Sensor Toward Quinolone Antibiotics
Authors: Lin Changjiao , Lu Tianle , Lu Xiaomei , Chen Zilu , Liu Dongcheng , Hu Huan‐Cheng 
Published: January 2025

ABSTRACTThe leakage of quinolone antibiotics may bring serious hazards to plants, animals, and human; thus, the detection of quinolone antibiotics is of great urgent yet remains challenging issue. Herein, two isostructural metal–organic frameworks {[(CH3)2NH2]2[M3(μ3‐O)(BPDC)3(TPP)]·xMeCN·yDMF}n (M = Co, x = 8, y = 1, NS‐13; M = Ni, x = 7, y = 1, NS‐14; H2BPDC = 4,4′‐biphenyldicarboxylic acid; TPP = 2,4,6‐tris(4‐pyridyl)pyridine), have been successfully prepared through solvothermal method. NS‐13 and NS‐14 were three‐dimensional networks built form [M3(μ3‐O)]4+ clusters. Impressively, both NS‐13 and NS‐14 displayed good fluorescence response to norfloxacin, ofloxacin, and enrofloxacin with low limit of detection (LOD) and excellent recyclability, to our best knowledge, which are superior to most of reported metal–organic frameworks. Furthermore, density functional theory (DFT) calculations and fluorescence lifetime experiments uncovered the possible fluorescence probe mechanism of NS‐13 and NS‐14 toward the above‐mentioned quinolone antibiotics. This work extends the application of cluster‐based metal–organic frameworks in the detection of antibiotics.
Pseudoagostic Interaction Based Zirconium Sensing Using 1,2,3‐Triazole and Its Anticarcinogenic Potential Evaluation Through In Vitro and In Silico Approach
Authors: Singh Gurjaspreet , Heena  , Khurana Sumesh , Mithun  , Gill Baljinder Singh , Baliyan Deepanjali , Vikas  , Dege Necmi , Tarcan Erdoğan 
Published: January 2025

ABSTRACTWith excellent yield, high selectivity, and sensitivity new 1,4‐disubstituted 1,2,3‐triazoles (8a) and (8b) were synthesized via Cu(I) catalyzed click reaction stitching the hydrazone tethered azide moiety with two different alkyne probes. They were subsequently characterized by 1H NMR, 13C NMR, mass spectrometry, and FT‐IR spectroscopy. In addition, X‐ray crystallography offered the full structural elucidation of compounds (4), (5), and (8a). The optical properties of the designed chemosensor (8a) towards Zr(IV) have been assessed through UV–visible and fluorescence spectroscopy. The LOD values obtained from absorption and emission spectroscopy are 50 and 0.90 nM, respectively. The pseudoagostic interaction between (8a) and Zr(IV) was established using 1H NMR, mass spectrometry, and FT‐IR spectroscopy. (8a) and (8b) exhibited good antioxidant properties with 0.7135 and 0.856 μM IC50 values and also revealed anticarcinogenic action in both in vitro and docking examinations, it may be anticipated that both probes have the potential to act as antitumor drugs.
Hydroxyethylcellulose and In Situ Formed Crosslinked Poly(Acrylamide‐Co‐2‐Acrylamido‐2‐Methylpropanesulfonic Acid) Based Calcium Ion Embedded Conductive Composite Hydrogels for Flexible Electronic Devices
Authors: Meng Lingling , Liu En , Li Weihao , Ding Shijie , Liu Da 
Published: January 2025

ABSTRACTIonic conductive hydrogels (ICHs) have received much attention as ideal candidates for flexible electronic devices. However, conventional ICHs have not been widely used due to their inability to simultaneously possess high toughness and high ionic conductivity. Here, an interpenetrating double network hydrogel designated as HPPC was prepared by photocopolymerizing AM, AMPS, and MBA in the presence of HEC and CaCl2 using irgacure as a photoinitiator. Hydrogen bonding between the HEC and P(AM‐AMPS) formed the interpenetrating network structure. Calcium ions functioned as ionic cross‐linking agents and conductive ions, effectively dissipating energy. A tensile strength of 0.101 MPa, 648% tensile strain, and satisfactory ionic conductivity were exhibited. In addition, the flexible strain sensor based on HEC/P(AM‐AMPS)/CaCl2 hydrogel has excellent sensitivity and can accurately record the movement status of different body parts of the human body. Notably, the specific capacitance of HEC/P(AM‐AMPS)/CaCl2‐CS can reach 30.5 F g−1 at a current density of 1 A g−1. Overall, this ion‐conductive hydrogel has potential for flexible electronics.
Risk Factors Influencing Cognitive Function in Elderly Patients With Late‐Life Depression: A Scoping Review
Authors: Jiang Ping , Gao Yunfeng , Wang Lin , Shao Xiaojun , Zhang Lei , Zhu Gang , Duan Li 
Published: January 2025

ABSTRACTBackgroundIn recent years, cognitive impairment has emerged as a pivotal symptom in elderly patients with depression, exerting a substantial impact on the course and prognosis of diseases. Moreover, it persists even following remission from depression during the rehabilitation period. However, there remains an incomplete understanding of the relevant influencing factors for cognitive impairment in elderly depressed patients, which seriously impedes the development of risk prediction models and the subsequent research on precision intervention programs.ObjectiveThe purpose of this study is to examine the current state of negative influencing factors and assessment tools for cognitive impairment in patients with late‐life depression (LLD), thereby providing a theoretical framework for the construction of subsequent targeted intervention programs.MethodsThe search strategy employed in this study followed an evidence‐based approach, utilizing a systematic scoping review to thoroughly explore six English and four Chinese databases up until November 2023. Two researchers independently conducted article screening and employed thematic analysis to categorize the results into themes.ResultsFollowing two rounds of rigorous screening conducted by the evidence‐based research team, data were meticulously extracted and succinctly summarized from five distinct themes encompassing socio‐demographic, physiological, psychological, genetic, and other related factors. In addition, a comprehensive compilation of 19 diverse assessment tools was undertaken. Ultimately, a total of 22 articles met the eligibility criteria for inclusion in this study. These comprised five longitudinal studies, nine pathological controlled studies, five cross‐sectional studies, two cohort studies, and one randomized controlled study.ConclusionCognitive dysfunction is an important symptom of LLD, which seriously affects the survival of patients. At present, the research on its influencing factors mainly includes socio‐demographic, physiological, psychological, genetic, and other related factors. There have been existing cognitive function assessment tools specifically for those 18‐ to 65‐year‐old patients of major depressive disorder, but there is still a lack of reliability and validity tests in LLD.
Corporate Environmentalism and Business Sustainability in Ghana: Prospects and Challenges
Authors: Ahenkan Albert , Boon Emmanuel , Akalibey Scholastica , Nordjo Eric 
Published: January 2025

ABSTRACTThe concept of corporate environmentalism (CE) has gained a lot of global attention in recent years as a potential vehicle to achieve sustainable development. Despite the growing awareness of its importance, there is limited empirical research on the current state of sustainability practices and their implementation among businesses in Ghana. Using a multiple case study design grounded in the qualitative approach, the study dwells on desk reviews of existing literature, corporate sustainability reports, and 10 key informant interviews including policymakers, regulators, academics, and practitioners from diverse industries; this study seeks to examine the (i) drivers and motivators, (ii) prospects, (iii) challenges, and (iv) approaches to scale up CE in Ghana. The results of the study revealed that branding, alignment with the sustainable development agenda, commitment to climate action, stakeholder and regulatory pressure, business resilience, and competitiveness are key drivers motivating companies to adopt environmental sustainability practices. The study identified prospects of CE for companies in Ghana including market demand and consumer awareness. Cost savings and operational efficiency offer significant potential, and access to finance presents opportunities for green initiatives. Reputation and brand enhancement are achievable through sustainability efforts but require consistent communication and stakeholder engagement. However, despite growing momentum, companies face challenges including financial constraints, limited access to environmentally friendly technologies, lack of technical expertise, and weak enforcement of environmental regulations. To overcome these obstacles, the article proposes a number of strategies to advance CE in Ghana.
Methods for Defining Climate Change Mitigation Strategies at Organisational Level Review and Outlook
Authors: Desmoitier Nicolas , Roman Jean , Hilloulin Benoit , Rozière Emmanuel , Laurent Alexis 
Published: January 2025

ABSTRACTThe private sector has a pivotal role in contributing to the objective of the Paris Agreement and its 1.5° target. However, there is a lack of a comprehensive framework to define and implement climate change mitigation strategies in organisations. The current study aims to fill this gap by performing a critical review of existing schemes that can make a partial contribution to the definition of such strategies. We found 25 schemes used by private sector or studied in the literature by international NGOs and academic searcher and evaluated them based on 15 criteria developed for this study in order to cover key components of an effective framework for CCMS in organisations. No scheme fulfils all the criteria, but significant differences are observed between them, ranging from 0% to 80% of the criteria met. Based on this analysis, we propose a first harmonized five‐step iterative framework to define organisational climate change mitigation strategies. Eventually, the results of the schemes review and the framework proposal draw up a research agenda to derive robust, credible and operational climate strategies at organizational levels.
Mutation in CDC42 Gene Set as a Response Biomarker for Immune Checkpoint Inhibitor Therapy
Authors: Wang Kun , Zhang Yingying , Su Zhaoming , Wang Bei , Zhou Yuanyang , Tong Xiaochu , Xie Chengying , Luo Xiaomin , Zhang Sulin , Zheng Mingyue 
Published: January 2025

ABSTRACTBackgroundImmune checkpoint inhibitors (ICIs) have achieved great success; however, a subset of patients exhibits no response. Consequently, there is a critical need for reliable predictive biomarkers. Our focus is on CDC42, which stimulates multiple signaling pathways promoting tumor growth. We hypothesize that an impaired function of CDC42 may serve as an indicator of a patient's response to ICI therapy.MethodsWe consider CDC42 and its downstream binding and effector proteins as a gene set, as mutations in these components could lead to defective CDC42 function. To elucidate the biomarker function of mutations within the CDC42 gene set, we curated a comprehensive discovery dataset that included seven ICI treatment cohorts. And we curated two ICI treatment cohorts for validation. We explored the mechanism based on The Cancer Genome Atlas database. We also examined whether combining a CDC42 inhibitor with ICI could enhance ICI's efficacy.ResultsMutations in the CDC42 gene set were associated with improved overall survival and progression‐free survival. Furthermore, our analysis of immune response landscapes among different statuses of the CDC42 gene set supports its role as a biomarker. Animal experiments also revealed that the combination of the CDC42 inhibitor (ML141) with anti‐PD‐1 blockade can additively reduce tumor growth.ConclusionsOur study suggests that the CDC42 gene set mutations could potentially serve as a novel biomarker for the clinical response to ICI treatment. This finding also provides insights into the potential of combining ICI and CDC42 inhibitor use for more efficient patient treatment.
Design and Characterization of a Micro RNA‐200c Detecting Broccoli Fluorescent Light‐up Aptamer
Authors: Kersten Corinna , Zahler Stefan , Schneider Sabine 
Published: January 2025

AbstractIn the last decade the important role of small non‐coding RNAs such as micro RNAs (miRs) in gene regulation in healthy and disease states became more and more evident. The miR‐200‐family of miRs has been shown to play a critical role in many diseases such as cancer and neurodegenerative disorders and could be potentially important for diagnosis and treatment. However, the size of miRs of about ~21–23 nt provide challenges for their investigation. Here we report the conversion and optimization of the Broccoli fluorescent light‐up RNA‐aptamer into a specific sensor for miR‐200c using a strand‐displacement design principle. This aptamer can differentiate miR‐200c from its family members whose sequence differ by more than one nucleotide. By adding this in vitro transcribed aptamer to RNA extracts from human cells, we can detect miR‐200c in vitro in a plate reader assay.
Microsurgical Semilunar Bilaminar Technique Halts Progression of Marginal Tissue Recession by Soft Tissue Phenotypic Modification—A Case Report With 3‐Year Follow‐Up
Authors: Sampoornam Pape Reddy Saravanan , Francis Delfin Lovelina , Rathi Manish , Harish Ruchi , Narayana Ravikiran , Manohar Balaji , Pradhan Shaili 
Published: January 2025

ABSTRACTPhenotype modification therapy using the novel semilunar flap with xenogeneic collagen matrix improves gingival phenotype, root coverage and increased keratinized tissue width. It also halts the progression of recession, enhances tissue resilience and improved plaque control sustainable over 3 years. Collagen matrix avoids second site surgery and hence less morbidity.