bluuebunny/crossref_metadata_2025_split huggingface dataset:
The combination of GS-441524 (remdesivir) and ribavirin results in a potent antiviral effect against human parainfluenza virus 3 infection in human airway epithelial cell cultures and in a mouse infection model
Authors: Lin Yuxia , Weynand Birgit , Zhang Xin , Laporte Manon , Jochmans Dirk , Neyts Johan 
Published: January 2025

AbstractHuman parainfluenza virus type 3 (HPIV-3) can cause severe respiratory diseases, particularly in young children, the elderly and immunocompromised. There are no approved antiviral drugs against this virus. We report that the combination of ribavirin with either remdesivir or its parent nucleoside GS-441524 results in a pronounced antiviral effect against HPIV-3 in LLC-MK2 cells and in human airway epithelial cells grown at the air-liquid interface. In AG129 mice intranasally inoculated with HPIV-3, the combined treatment of ribavirin and GS-441524 decreases infectious viral lung titers by >2.5 log10to undetectable levels in 4 out of 11 mice and by 1.6 log10in the remaining 7 mice as compared with vehicle. The lungs of all mice that received the combined treatment appear histologically normal or virtually normal, whereas 8 of 11 vehicle-treated mice present with bronchopneumonia. By contrast, ribavirin alone does not result in a reduction of infectious viral lung titers; GS-441524 alone reduces infectious viral lung titers by 1.2 log10. Moreover, several mice in the single-treatment groups exhibit severe lung pathology. These findings may warrant exploring this combination in patients with severe HPIV-3 infections and possibly also against infections with other viruses that arein vitrosusceptible to these two drugs.
Maturation of striatal dopamine supports the development of habitual behavior through adolescence
Authors: Petrie Daniel J. , Parr Ashley C. , Sydnor Valerie , Ojha Amar , Foran Will , Tervo-Clemmens Brenden , Calabro Finnegan , Luna Beatriz 
Published: January 2025

AbstractDevelopmental trajectories during the transition from adolescence to adulthood contribute to the establishment of stable, adult forms of operation. Understanding the neural mechanisms underlying this transition is crucial for identifying variability in normal development and the onset of psychiatric disorders, which typically emerge during this time. Habitual behaviors can serve as a model for understanding brain mechanisms underlying the stabilization of adult behavior, while also conferring risk for psychopathologies. Dopaminergic (DA) processes in the basal ganglia are thought to facilitate the formation of habits; however, developmental trajectories of habits and the brain systems supporting them have not been characterizedin vivoin developing humans. The current study examined trajectories of habitual behavior from adolescence to adulthood and sought to understand how the maturing striatal DA system may act as a potential mechanism in the process of habit formation. We used data from two longitudinal studies (combinedn= 217, 10 – 32 years of age, 1-3 visits each, 320 total sessions) to characterize normative developmental trajectories of basal ganglia tissue iron concentration (a proxy for DA-related neurophysiology) and goal-direct and habitual control behaviors in a two-stage decision-making task. Tissue iron concentrations across the basal ganglia and habitual responding during the two-stage sequential decision-making task both increased with age (allp< 0.001). Importantly, habitual responding was associated with tissue iron concentrations in the putamen (F= 4.34,p= 0.014), such that increases in habitual responding were supported by increases in putamen tissue iron concentration during childhood through late adolescence. Exploratory analyses of further subdivisions of anatomical regions found that this association was specific to the posterior putamen. These results provide novel evidence in humans that habitual behavior continues to mature into adulthood and may be supported by increased specialization of reward systems.
Psilocybin induces sex- and context-specific recruitment of the stress axis
Authors: Cook Sarah Gibson , Lee Stephanie , Ference Emma , Shi Yuecheng , Rojas-Carvajal Mijail , Hen Rene , Bains Jaideep S. , Füzesi Tamás , Turi Gergely F. 
Published: January 2025

ABSTRACTPsychedelics have reemerged as potential treatments for mental health disorders, yet their impact on stress-related brain regions remains poorly understood. Here, we provide the first real-time,in vivoevidence of psilocybin-induced neuronal activation, specifically in hypothalamic corticotropin-releasing hormone neurons. Notably, psilocybin elicited more pronounced responses in female mice and produced context-related alterations in threat assessment. Our findings provide valuable insight into the impact of psychedelics on a key stress center in the brain.
Structure and catalytic mechanism of methylisocitrate lyase, a potential drug target against Coxiella burnetii
Authors: Stuart William S. , Jenkins Christopher H. , Ireland Philip M. , Isupov Michail N. , Norville Isobel H. , Harmer Nicholas J. 
Published: January 2025

AbstractWe present a comprehensive investigation into the catalytic mechanism of methylisocitrate lyase, a potential drug target candidate against the zoonotic pathogenCoxiella burnetii, the causative agent of Q fever and a federal select agent. Current treatment regimens are prolonged, often with incomplete clearance of the pathogen. We utilised a structure-based bioinformatics pipeline to identify methylisocitrate lyase as a candidate therapeutic target againstC. burnetiifrom a list of essential genes. Wild-typeC. burnetiimethylisocitrate lyase has akcatof 32,000 s-1(compared to 105 s-1forSalmonella enterica) and isocitrate inhibits with aKIof 6 mM. We have determined the previously uncharacterised substrate-bound structure of this enzyme family, alongside product and inhibitor-bound structures. These structures of wild-type enzyme reveal that in the active state the catalytic C118 is positioned 2.98 Å from O5 of methylisocitrate and Arg152 moves towards the substrate relative to the inhibitor bound structure. Analysis of structure-based mutants reveals that Arg152 and Glu110 are both essential for catalysis. We suggest that Arg152 acts as the catalytic base that initiates the methylisocitrate lyase reaction. These results deepen our understanding of the catalytic mechanism of methylisocitrate lyase and could aid the development of new therapeutics againstC. burnetii.
Mapping Early Brain Maturation: Anatomical Substrates of Cortical Connectivity Shifts in Neonates
Authors: Li Deying , Wang Yufan , Cheng Luqi , Eickhoff Simon B , Chu Congying , Fan Lingzhong 
Published: January 2025

AbstractUnderstanding the early development of brain connectivity is essential for unraveling the mechanisms of brain maturation. In this study, we utilized diffusion MRI data from 242 term neonates to chart the developmental trajectory of cortical connectivity in infants. We quantified interareal variations in connectivity and identified three distinct global connectopies (GCs) along the rostrocaudal, dorsoventral, and mediolateral axes by using gradient mapping techniques. A pivotal age-dependent shift between the dorsoventral and mediolateral GCs occurred at 40 postmenstrual weeks. This shift was diminished by virtual thalamic fiber lesions, underscoring the critical role of thalamic projections. A longitudinal analysis disclosed a delayed structural connectivity pattern development in preterm infants who failed to exhibit the anticipated shift. This research helps to clarify the topographic principles that shape infant brain organization and emphasizes the influence of thalamic connectivity on early brain development. Our findings elucidate the anatomical underpinnings of early brain development, providing critical insights into the formation of cortical hierarchies and highlighting the influence of thalamic connectivity on developmental trajectories. These findings are essential for understanding typical brain growth and the pathogenesis of neurodevelopmental disorders.
Attentional cueing effects are reversed during locomotion
Authors: Djebbara Zakaria , Chau Dylan , Koselevs Aleksandrs , Chen Yiru , Fich Lars Brorson , Gramann Klaus 
Published: January 2025

AbstractEveryday human cognition and behaviour evolved in dynamic and ever-changing environments, but static paradigms still dominate experimental research despite concerns about generalisability of the results. In the case of attention, traditional stationary studies show that pre-orienting attention with spatial cues leads to faster, more accurate responses. However, how movement and environmental features shape such attentional processes in everyday behaviour remains unknown. Here we show that active movement through curved corridors reverses the typical spatial attention effect, with faster response times and higher accuracy for stimuli incongruent to implicit spatial cues provided by the movement direction, contradicting previous findings from static settings. We found that early (N1) and late (P3) attention-related electrophysiological responses were modulated by environmental features and motor demands. The posterior N1-component, reflecting visuo-spatial attention, showed decreasing amplitudes as turning angles and motor-control demands increased for congruent stimuli appearing on the side of the turning direction. Similarly, the P3-complex varied with motor and visual processing demands for congruent stimuli, showing decreased amplitudes as motor-control demands increased. We propose that congruent stimuli, displayed against a dynamically changing visual context, increase pulvino-cortical processing load and slowing early visual processing that affect behavioural responses. Incongruent stimuli, however, are displayed against a predictable context allowing faster target processing. These findings challenge attentional mechanisms’ assumed consistency across static and dynamic settings, revealing instead their dependence on behavioural and environmental context. We advocate for naturalistic paradigms, arguing that moving beyond static experiments could reshape core views on cognition and behaviour.
Dorsal hippocampus mediates light-tone sensory preconditioning task in mice
Authors: Pinho Julia S. , Ramon-Duaso Carla , Manzanares-Sierra Irene , Busquets-García Arnau 
Published: January 2025

AbstractDaily choices are often influenced by environmental cues that are not directly linked to reinforcers. This process is known as higher-order conditioning and can be measured using sensory preconditioning tasks in rodents. This behavioral paradigm requires the repeated and simultaneous presentation of two low-salience stimuli, such as a light and a tone, followed by a devaluation phase where one stimulus is paired with an unconditioned stimulus, such as a mild footshock. The outcome is a conditioned response (i.e. freezing response) to both the conditioned stimulus (direct learning) and the non-conditioned stimulus (mediated learning). In our study, we set up a successful light-tone sensory preconditioning task in male and female mice. Sex differences were seen on the number of conditioning sessions required to acquire mediated learning and in the behavioral responses observed in certain control experimental groups. We usedin vivocalcium imaging to characterize the activity of hippocampal neurons in the dorsal and ventral subregions of the hippocampus when associations between low-salience stimuli and reinforcers occur. Finally, we combined our sensory preconditioning task with chemogenetic approaches to assess the role of these two hippocampal subregions in mediated learning. Our results indicate that dorsal, but not ventral, CaMKII-positive cells mediate the encoding of low-salience stimuli during the preconditioning phase. Overall, we implemented a novel light-tone sensory preconditioning protocol in mice that allowed us to detect sex differences and to further elucidate the role of particular hippocampal subregions and cell types in regulating these complex cognitive processes.
Human sleep spindles track experimentally excited brain circuits
Authors: Thom Jude L. , Staresina Bernhard P. 
Published: January 2025

AbstractSpindles are hallmark oscillations during non-rapid-eye-movement (NREM) sleep. Together with slow oscillations (SOs), they are thought to play a mechanistic role in the consolidation of learned information. The quantity and spatial distribution of spindles has been linked to brain activity during learning before sleep and to memory performance after sleep. If spindles are drawn to cortical areas excited through pre-sleep learning tasks, this begs the question whether the spatial distribution of spindles is flexible, and whether their regional expression can also be manipulated with experimental brain stimulation. We used excitatory transcranial direct current stimulation (tDCS) to stimulate the left and right motor cortex in a repeated-measures experimental design. After stimulation, we recorded high-density electroencephalography (EEG) during sleep to test how local stimulation modulated the regional expression of sleep spindles. Indeed, we show that excitatory tDCS of local cortical sites before sleep biases the expression of spindles to the excited locations during subsequent sleep. No effects of localised tDCS excitation were seen for SOs. These results demonstrate that the spatial topography of sleep spindles is neither hard-wired nor random, with spindles being flexibly directed to exogenously excited cortical circuits.Graphical AbstractStatement of SignificanceSpindles are signatures of NREM sleep linked to memory consolidation, intelligence, and neurological disorders. Recent work shows the spatial distribution of spindles mirrors cortical activation patterns during learning. If spindles are preferentially expressed at cortical sites engaged during prior waking, the question arises whether spindles can be directed by inducing excitation exogenously with non-invasive brain stimulation. Here, we applied anodal-tDCS to either left or right motor areas before a nap. Indeed, we found that spindle (and not SO) rates are greater at cortical sites excited by tDCS. This result suggests the spatial distribution of spindles is flexible and is sensitive to experimental excitatory stimulation. This has implications for targeted rehabilitation of specific cortical areas and for developing sleep-based brain-computer interfaces.
Loss of Elp3 impairs the maturation tempo of brain ependymal cells
Authors: Tielens S. , Boutin C. , Huysseune S. , Thelen N. , Charlet-Briart M. , Reyskens C. , Oskera L. , Boutsen A. , Mateo-Sanchez S. , Delacroix L. , Labedan P. , Thiry M. , Malgrange B. , Chariot A. , Tissir F. , Creppe C. , Seuntjens E. , Laguesse S. , Nguyen L 
Published: January 2025

AbstractConditional deletion ofElp3in the mouse forebrain leads to microcephaly at birth. In this study, we demonstrate that these mice also develop postnatal hydrocephalus, associated with an enlargement of the brain ventricles. In wild-type mice, ependymal motile cilia are properly aligned to facilitate the circulation of cerebrospinal fluid (CSF) within the ventricles. Our findings reveal thatElp3loss induces endoplasmic reticulum (ER) stress and upregulation of ATF4 expression in ependymal cell progenitors, which compromises Notch signaling and accelerates their maturation. This is accompanied by a disruption in the establishment of rotational and translational polarities of the motile cilia of maturing ependymal cells, resulting in disorganized cilia bundles. Collectively, these molecular abnormalities lead to the premature and abnormal development of ependymal cells, culminating in cilia beating dysfunction, impaired CSF clearance, and the development of hydrocephalus.
Match or mismatch: how emoji use shapes consumers’ perception of online reviews
Authors: Cai Zhenchuan , Chen Kaili , Zhao Haichuan 
Published: January 2025

PurposeThe purpose of this research is to explore how text-emoji mismatch impacts consumers’ perceptions of authenticity and helpfulness in online reviews, examining the mediating effect of processing fluency and the moderating effects of response type and social distance.Design/methodology/approachThree between-subject experiments were conducted on Credamo and Wenjuanxing to test the hypotheses proposed in this research. The primary data analysis methods used were ANOVA and bootstrap analysis.FindingsStudy 1 found that consumers’ perceived helpfulness and authenticity of online reviews decline when the text and emojis do not match, with processing fluency mediating this effect. Study 2 revealed that humorous responses from firms to reviews with text-emoji mismatches enhance consumers’ perceived authenticity and helpfulness. Study 3 demonstrated that when potential consumers perceive a greater social distance from the reviewers, the negative impact of text-emoji mismatch is mitigated.Originality/valueThis study contributes to the fields of consumer reviews and emoji usage by examining how the relationship between emojis and text in online reviews (match vs. mismatch) affects consumers’ perceived authenticity and helpfulness. We also propose strategies for how firms can mitigate the negative impact of text-emoji mismatch.
The causes of electronic word of mouth on food sharing platforms: the perspective of knowledge sharing motivations
Authors: Leong Han-Yee , Chao Ren-Fang , Wu Tzu-Ching 
Published: January 2025

PurposeThis study explores how egoism and altruism as knowledge sharing motivations influence consumers’ electronic word of mouth (eWOM) intentions on food platforms. It also investigates the interaction effect of egoism and altruism on eWOM intentions through attitude toward use (ATU).Design/methodology/approachA survey was conducted with 523 consumers who posted reviews on Taiwan’s two leading food platforms. The data were analyzed using structural equation modeling to examine the relationships between knowledge sharing motivations, ATU, and eWOM intentions.FindingsEgoism and altruism influence eWOM intentions through ATU, with altruism having a more substantial impact. Their interaction further enhances the positive relationship between ATU and eWOM intentions.Originality/valueThis study fills the empirical gap by demonstrating the interaction effect of egoism and altruism on eWOM intentions. It highlights the role of intrinsic motivations in shaping consumer behavior on digital platforms and offers insights for businesses aiming to foster consumer engagement.
Exploring the landscape of libraries on smart devices: a systematic literature review using PRISMA
Authors: Margam Madhusudhan , Pandey Parbati 
Published: January 2025

Purpose
The systematic literature review aims to highlight the transformative influence of mobile technology in reshaping library services for Library and Information Science (LIS) professionals and users.

Design/methodology/approach
The selection of relevant literature utilized the PRISMA-20 guidelines and a systematic literature review, wherein 39 articles were meticulously examined from a pool of 198. The inclusion and exclusion criteria were applied to articles retrieved from the Scopus database and Google Scholar, focusing on data from 2018 to 2023 and excluding all the literature except journal articles.

Findings
The results demonstrate a significant awareness among LIS professionals and users regarding adopting mobile-based library services. Nonetheless, the adoption of mobile technology in libraries faces impediments from internal and external factors. These challenges encompass the need for coherent policies and practical issues such as insufficient financial resources, limitations in infrastructure, unpredictable electricity and Internet connectivity. The study also highlights concerns related to data security, the digital divide, a shortage of IT experts and inadequate staffing. The findings suggest that libraries must navigate this evolving landscape to provide improved and more accessible services to their users.

Practical implications
This study addresses a gap in the existing research by contributing to the literature on using mobile technology for accessing library services and resources. The findings aim to assist LIS professionals in developing plans, policies and strategies for implementing mobile-based library services.

Originality/value
The results of this study contribute to improving and expanding current mobile-based services in libraries, ensuring that information and resources are universally accessible “on the go environment.”
Women’s career and imposter syndrome: moderating effects of female role model and diversity management in the hospitality and tourism sector
Authors: Forson Mavis Adjoa , Hao Fei , Cheung Catherine 
Published: January 2025

Purpose
This study aims to examine the influence of imposter syndrome on women’s careers in the hospitality and tourism industry drawing on the role congruity theory of prejudice.

Design/methodology/approach
Employing cross-sectional design, this research analyzed quantitative data from 574 women at various managerial levels using the Structural Equation Modeling (SEM) technique.

Findings
The results show that imposter syndrome significantly impacts women employees’ engagement and organizational commitment. The study found that higher levels of diversity management and female role models have a stronger moderating effect on the relationship between imposter syndrome, career expectations, work-family balance, employee engagement, career advancement and organizational commitment whilst lower levels of diversity management and female role models have a weaker moderating effect on these relationships.

Research limitations/implications
This study presents a new theoretical perspective on female employees’ challenges to career advancement. It expands the knowledge of imposter phenomenon and role congruity theory of prejudice, illustrating how imposter syndrome can be influenced not only by individual traits but also by institutional, situational or stereotypical factors.

Practical implications
This work provides valuable insights for hotel policymakers and managers to implement policies that alleviate imposter syndrome and contribute to women’s career development and gender equality in workplaces.

Originality/value
This study can serve to raise awareness of women’s issues in the workplace and offer insightful guidance to organizations to promote diversity and women’s leadership. These pertain to how realistic human resources policies can be used to promote the well-being of hospitality and tourism women employees.