bluuebunny/crossref_metadata_2025_split huggingface dataset:
Exaggerated myocardial torsion may contribute to dynamic left ventricular outflow tract obstruction in hypertrophic cardiomyopathy
Authors: Lo Ada K C , Mew Thomas , Mew Christina , Guppy-Coles Kristyan , Dahiya Arun , Ng Arnold , Prasad Sandhir , Atherton John J 
Published: August 2023

Abstract

Aims
Dynamic left ventricular (LV) outflow tract obstruction (LVOTO) is associated with symptoms and increased risk of developing heart failure in hypertrophic cardiomyopathy (HCM). The association of LVOTO and LV twist mechanics has not been well studied in HCM. The aim of the study was to compare the pattern of LV twist in patients with HCM associated with asymmetrical septal hypertrophy with and without LVOTO.

Methods and results
Echocardiography (including speckle tracking) was performed in 212 patients with HCM, divided according to the absence (n = 130) or presence (n = 82) of LVOTO (defined as peak pressure gradient ≥30 mmHg either at rest and/or with Valsalva manoeuvre). Patients with LVOTO were older, had smaller LV dimensions, a higher LV ejection fraction (LVEF), a longer anterior mitral valve leaflet length, and a higher early transmitral pulsed wave to septal tissue Doppler velocity ratio (E/E′). A univariate analysis showed that peak twist was significantly higher in patients with LVOTO compared with patients without LVOTO (19.7 ± 7.3 vs. 15.7 ± 6.0, P = 0.00015). Peak twist was similarly enhanced in patients with LVOTO, manifesting only during Valsalva (19.2 ± 5.6, P = 0.007) and patients with resting LVOTO (19.9 ± 8.0, P = 0.00004) compared with patients without LVOTO (15.7 ± 6.0). A stepwise forward logistic regression analysis showed that LVEF, LV end-systolic dimension indexed to body surface area, anterior mitral valve leaflet length, E/E′, and peak twist were all independently associated with LVOTO.

Conclusion
This study demonstrates that increased peak LV twist is independently associated with LVOTO in patients with HCM. Peak twist was similarly exaggerated in patients with only latent LVOTO, suggesting that it may play a contributory role to LVOTO in HCM.
Subgroup analyses in clinical research: too tempting?
Authors: Groenwold Rolf H H , Dekkers Olaf M 
Published: August 2023

Abstract
In many biomedical studies, subgroup analyses are performed to identify subgroups of patients in whom a treatment is most effective, or a risk factor has the largest effect. While both are referred to as subgroup analysis, it is important to distinguish between the estimation of effects within subgroups and the comparison of effects across subgroups. Both are discussed, and we outline the implications regarding sample size and statistical methods for estimation of effects. Also, the risk of false-positive findings—which potentially increases with subgroup analysis—is discussed, as well as the distinction between effect modification and interaction.
Acute Exercise Effect on Neurocognitive Function Among Cognitively Normal Late-Middle-Aged Adults With/Without Genetic Risk of AD: The Moderating Role of Exercise Volume and APOE Genotype
Authors: Chang Yu-Kai , Etnier Jennifer L , Li Ruei-Hong , Ren Fei-Fei , Ai Jing-Yi , Chu Chien-Heng 
Published: August 2023

Abstract

Background
Acute exercise is a behavior that benefits cognitive function; however, its effect on populations with different risks for Alzheimer’s disease (AD) and the role of exercise variance and Apolipoprotein E (APOE) genotype on this effect remains unknown. This study explores the acute exercise effect on behavioral and neurocognitive function, and its potential moderation by exercise intensity and duration and APOE genetic risk.

Methods
Fifty-one cognitively normal adults (~36% APOE ε4 carriers) performed the Stroop task under a rest condition and 3 exercise conditions while electroencephalographic activity was assessed.

Results
Acute exercise improved cognitive performance assessed through both behavioral and neuroelectrical indices. These benefits were observed regardless of adjustments of intensity and duration at a predetermined exercise volume as well as being evident irrespective of APOE ɛ4 carrier status.

Conclusions
Acute exercise could be proposed as a lifestyle intervention to benefit neurocognitive function in populations with and without genetic risk of AD. Future exploration should further the precise exercise prescription and also the mechanisms underlying the beneficial effects of acute exercise for neurocognitive function.

Clinical Trials Registration Number
NCT05591313
Decision Making for Patients With Severe Dementia Versus Normal Cognition Near the End of Life
Authors: Nicholas Lauren Hersch , Halpern Scott D , Weir David R , Baum Micah Y , Nolan Marie , Gallo Joseph , Langa Kenneth M 
Published: August 2023

Abstract

Background and Objectives
The clinical progression of severe dementia frequently leads to situations where surrogate decision makers must quickly make choices about potentially burdensome treatments that offer limited clinical benefit. We examined whether the number of decision makers and their access to advance directives were related to treatment choice for patients with severe dementia in comparison to those with normal cognition.

Research Design and Methods
We retrospectively linked survey responses about end-of-life treatment decisions to Medicare claims for Health and Retirement Study respondents dying between 2002 and 2015 whose next-of-kin reported a need for surrogate decision making. We estimated multivariable logistic regression models to study measures of aggressive care in the last 6 months of life; in-hospital death, burdensome transfers, and burdensome treatments.

Results
Compared to patients who were cognitively normal near the end of life (n = 1 198), patients with severe dementia (n = 722) were less likely to experience burdensome treatments (18% [95% confidence interval {CI} 14–21] vs 32% [95% CI 29–35]), burdensome transfers (20% [95% CI 17–24] vs 30% [95% CI 27–33]), and in-hospital death (24% [95% CI 20–28] vs 30% [95% CI 26–33]) when surrogates were involved. Rates of burdensome treatments, transfers, or in-hospital death for decedents with severe dementia did not vary with single versus multiple decision makers or when decision makers were informed by advance directives. However, among decedents with normal cognition, a single decision maker informed by an advance directive was associated with the lowest rates of burdensome treatments and in-hospital death.

Discussion and Implications
Surrogate decision makers made similar choices around end-of-life care for patients with severe dementia regardless of the number of decision makers and availability of advance directives. However, both advance directives and single decision makers were associated with less aggressive care for cognitively normal decedents.
Social Sustainability in Aging Populations: A Systematic Literature Review
Authors: Komp-Leukkunen Kathrin , Sarasma Juho 
Published: August 2023

Abstract

Background and Objectives
Social sustainability becomes increasingly important in aging populations. Yet, scientific discussions on this topic are still emerging. This study helps to develop these discussions by exploring (1) how social sustainability is understood in studies on aging populations, (2) how this understanding differs across the topics discussed, and (3) how population aging is connected to social sustainability.

Research Design and Methods
This study conducts a systematic literature review using 33 texts obtained from ProQuest, JStor, and scholar.google.com (02–03/2022). The inclusion criteria were (1) scientific character and (2) explicit focus on the topics of interest. A thematic analysis was conducted.

Results
The texts use 3 different understandings of social sustainability: one focusing on what makes societies desirable, one focusing on the quality of life of individuals, and one balancing the interests of current and future generations. The first understanding is most prevalent. Which understanding texts choose depends on their topic, perspective, and goals. The texts describe challenges and opportunities for social sustainability in aging populations, with some recommending a general shift in perspective.

Discussion and Implications
Findings provide a clearer and more homogenous understanding of social sustainability for discussions on population aging. Thereby, they facilitate a dialogue between researchers working in this area. Moreover, they help gerontologists increase their contribution to cumulative knowledge building. A limitation is that only texts in English are analyzed. Findings help policymakers and practitioners better understand how to integrate research-based knowledge on social sustainability in their work.
Genomic basis of selective breeding from the closest wild relative of large-fruited tomato
Authors: Yang Junwei , Liu Yun , Liang Bin , Yang Qinqin , Li Xuecheng , Chen Jiacai , Li Hongwei , Lyu Yaqing , Lin Tao 
Published: August 2023

Abstract
The long and intricate domestication history of the tomato (Solanum lycopersicum) includes selection sweeps that have not been fully explored, and these sweeps show significant evolutionary trajectories of domestication traits. Using three distinct selection strategies, we represented comprehensive selected sweeps from 53 Solanum pimpinellifolium (PIM) and 166 S. lycopersicum (BIG) accessions, which are defined as pseudo-domestication in this study. We identified 390 potential selection sweeps, some of which had a significant impact on fruit-related traits and were crucial to the pseudo-domestication process. During tomato pseudo-domestication, we discovered a minor–effect allele of the SlLEA gene related to fruit weight (FW), as well as the major haplotypes of fw2.2/cell number regulator (CNR), fw3.2/SlKLUH, and fw11.3/cell size regulator (CSR) in cultivars. Furthermore, 18 loci were found to be significantly associated with FW and six fruit-related agronomic traits in genome-wide association studies. By examining population differentiation, we identified the causative variation underlying the divergence of fruit flavonoids across the large-fruited tomatoes and validated BRI1-EMS-SUPPRESSOR 1.2 (SlBES1.2), a gene that may affect flavonoid content by modulating the MYB12 expression profile. Our results provide new research routes for the genetic basis of fruit traits and excellent genomic resources for tomato genomics-assisted breeding.
Effects of dietary protein content and crystalline amino acid supplementation patterns in low protein diets on intestinal bacteria and their metabolites in weaned pigs raised under Different sanitary conditions
Authors: Lee Jinyoung , Htoo John K , Kluenemann Martina , González-Vega J Caroline , Nyachoti Charles Martin 
Published: August 2023

Abstract
The objective of this experiment was to investigate the effects of dietary crude protein (CP) content and crystalline amino acids (CAA) supplementation patterns in low CP (LCP) diets on intestinal bacteria and their metabolites in weaned pigs raised under clean (CSC) or unclean sanitary conditions (USC). One hundred forty-four piglets (6.35 ± 0.63 kg) were assigned to one of six treatments in a 3 × 2 factorial arrangement based on CP content and sanitary conditions in a randomized complete block design to give eight replicates with three pigs per pen over a 21-d period. Diets consisted of a high CP (HCP; 21%) and two LCP (18%) diets supplemented with 9 CAA (Lys, Met, Thr, Trp, Val, Ile, Leu, His, and Phe) or only six CAA (Lys, Met, Thr, Trp, Val, and Ile) to meet the requirements. The CSC room was washed weekly, whereas the USC room had sow manure spread in the pens from the beginning of the study and was not washed throughout the experiment. Jejunum and colon digesta were sampled on day 21. Both jejunum and colon digesta were analyzed for ammonia nitrogen, short-chain fatty acids, and biogenic amines but only colon digesta was analyzed for microbiome composition (16s rRNA sequencing on MiSeq). Data were analyzed using R software for 16S rRNA and the MIXED procedure of SAS for microbial metabolites. Sanitation, CP content, and CAA supplementation patterns did not affect the diversity of colonic bacterial composition in weaned pigs. Pigs raised under USC had greater (P &lt; 0.05) jejunal ammonia nitrogen concentration than those raised under CSC. Pigs fed LCP diets had reduced (P &lt; 0.05) jejunal ammonia nitrogen concentration compared to those fed the HCP diet. Interactions between sanitation and dietary CP content were observed (P &lt; 0.05) for: (1) jejunal acetate and (2) colonic spermidine and spermine, whereby (1) acetate concentrations decreased from NCP to LCP in pigs raised under the CSC but those concentrations increased under the USC, and (2) spermidine and spermine concentrations increased in LCP diets compared to HCP diet under USC, unlike CSC which did not show any difference between HCP and LCP. In conclusion, reducing dietary CP lowered ammonia nitrogen content regardless of sanitation and increased microbial metabolites in weaned pigs raised under USC. However, LCP diets with different CAA supplementation patterns did not affect bacterial diversity in weaned pigs, regardless of the hygienic conditions where the animals were housed.
The effects of timing of high immune response phenotyping in relation to weaning on immune responses of crossbred beef calves
Authors: Beard Shannon C , Schmied Julie D , Hodgins Douglas C , Mallard Bonnie A 
Published: August 2023

Abstract
Genetic selection for immune response has the potential to increase the sustainability of the beef industry by breeding cattle that are productive yet with an increased capacity to resist disease. Determining the optimal time to immunophenotype beef cattle is crucial for the accurate prediction of an animal’s immune response. The objective of this study was to determine the effect of time of immunophenotyping in relation to weaning on immune responses of beef calves. Antibody- (AMIR) and cell-mediated (CMIR) immune responses were measured on 97 calves on the day of weaning (WEANING, N = 56) or 2 mo post-weaning (POST-WEANING, N = 41). Within each period of immunophenotyping, on day 0, blood was collected, and calves received a 1.0 mL intramuscular injection of type 1 and 2 test antigens. On day 14, blood was collected, and baseline skinfold thickness (SFT) was measured. Calves received an intradermal injection of 0.1 mg of the type 1 antigen suspended in 0.1 mL phosphate buffered saline (PBS) in the right tail fold, and 0.1 mL of PBS in the left. Changes in SFT at 24 h was used to indicate CMIR. To assess AMIR, the titer of type 2 antigen-specific bovine immunoglobulin G in serum from blood collected on day 14 was determined by measuring optical density (OD) using an enzyme-linked immunosorbent assay (ELISA). Among heifers, AMIR was greater for the POST-WEANING group than for the WEANING group (P &lt; 0.01). Among steers, AMIR was not different between the POST-WEANING group and the WEANING group (P = 1.0). Therefore, the AMIR of heifers may be more negatively affected by immunophenotyping at weaning than the AMIR of steers. For steers, CMIR was greater in the POST-WEANING group than the WEANING group (P &lt; 0.001). For heifers, CMIR was not different between the POST-WEANING group and the WEANING group (P = 0.22). The CMIR of steers may be more negatively affected by immunophenotyping at weaning than the CMIR of heifers. Calf age was not associated with AMIR or CMIR for calves phenotyped at weaning or post-weaning. The effect of sire nested within dam age was significant for CMIR for calves in the POST-WEANING group (P &lt; 0.01), but not for calves in the WEANING group (P = 0.67). The results suggest that measuring immunocompetence at weaning may not be representative of a calf’s genetic ability to mount an effective immune response, and immunophenotyping should be performed outside the weaning period.
Benchmarking global biodiversity of decapod crustaceans (Crustacea: Decapoda)
Authors: De Grave Sammy , Decock Wim , Dekeyzer Stefanie , Davie Peter J F , Fransen Charles H J M , Boyko Christopher B , Poore Gary C B , Macpherson Enrique , Ahyong Shane T , Crandall Keith A , de Mazancourt Valentin , Osawa Masayuki , Chan Tin-Yam , Ng Peter K L , Lemaitre Rafael , van der Meij Sancia E T , Santos Sandro 
Published: August 2023

AbstractA new assessment of the global biodiversity of decapod Crustacea (to 31 December 2022) records 17,229 species in 2,550 genera and 203 families. These figures are derived from a well-curated dataset maintained on the online platform DecaNet, a subsidiary of the World Register of Marine Species (WoRMS). Distinct phases are recognised in the discovery process (as measured by species descriptions) corresponding to major historical and geopolitical time periods, with the current rate of species descriptions being more than three times higher than in the Victorian age of global exploration. Future trends are briefly explored, and it is recognised that a large number of species remain to be discovered and described.
The effect of sunflower pollen age and origin on pathogen infection in the common eastern bumble bee (Apidae: Hymenoptera)
Authors: Fowler Alison E , Kola Elisa , Adler Lynn S 
Published: August 2023

Abstract
Bumble bees are globally important pollinators, contributing hundreds of millions of dollars annually in crop pollination services. Several species are in decline, making it paramount to understand how pathogens and nutrition shape bee health. Previous work has shown that consuming sunflower pollen (Helianthus annuus) dramatically reduces infection by the trypanosomatid gut pathogen, Crithidia bombi, in the common eastern bumble bee (Bombus impatiens). Sunflower pollen may therefore be useful as a dietary supplement for reducing this pathogen in managed bumble bee colonies. Here, we assessed the efficacy of freezer-stored sunflower pollen that was collected in different years and locations for reducing pathogen infection. We tested sunflower pollen that was 1, 3, 4, or 5 yr old and from sunflowers grown in the United States or China against a control of 1-yr-old buckwheat pollen from China, since buckwheat pollen results in high infection. We hypothesized that older pollen would have weaker medicinal effects due to degradation of pollen quality. We found that all sunflower pollen treatments significantly decreased Crithidia infection compared to controls. These results suggest that sunflower pollen can be freezer-stored for up to 5 yr and sourced from a wide range of geographic areas and still maintain its medicinal effects against Crithidia in the common eastern bumble bee. This is helpful information for stakeholders who might administer sunflower pollen as a dietary supplement to manage Crithidia in commercial bumble bee colonies.
Differential responses to double-stranded RNA injection and feeding in Mormon cricket (Orthoptera: Tettigoniidae)
Authors: Rana Seema , Kang Changsun , Allred Julianne , Medina-Duran Jorge Humberto , Canova Alyssa , Sherry Danielle , Woller Derek A , Kim Dongin , Song Hojun 
Published: August 2023

Abstract
The Mormon cricket, Anabrus simplex, is a flightless katydid, one of the major devastating rangeland pests in several states of the western United States. During the past few years, their sudden and periodic outbreaks into massive migratory bands caused significant economic losses to the rangeland forage and agricultural crops, particularly grain crops. Current population management methods rely heavily on broad-spectrum chemical insecticides, which could be toxic to nontargets, and even the targeted species might develop resistance in the long run. Therefore, we assessed the potential of RNA interference (RNAi)-based alternative management strategies that could supplement the current methods. In insects, RNAi efficiency varies with the method of double-stranded RNA (dsRNA) delivery. We tested 2 different methods of dsRNA delivery: injection and oral feeding of dsRNA. The results showed that Mormon crickets are sensitive to injection of dsRNA in a dose-dependent manner, but refractory to the oral feeding of dsRNA. Further, we confirmed the high nuclease activity in the insect midgut. In order to protect the dsRNA from the dsRNase activity and facilitate its uptake in the midgut, we encapsulated dsRNA inside poly lactic-co-glycolic acid (PLGA) nanoparticles and studied its release kinetics and RNAi efficiency by oral feeding. The release kinetics clearly suggested that the PLGA nanoparticle permeates from the insect digestive system to the hemolymph; however, it failed to induce an efficient RNAi response of the targeted genes. In conclusion, our findings suggest the different responses to dsRNA delivery methods in Mormon crickets, and further investigations involving dsRNA stability and its uptake mechanism are required to use RNAi as an alternative Mormon cricket population management strategy.
Trends and cancer-specific patterns of physical activity, sleep duration, and daily sitting time among US cancer survivors, 1997-2018
Authors: Cao Chao , Patel Alpa V , Liu Raymond , Cao Yin , Friedenreich Christine M , Yang Lin 
Published: August 2023

Abstract

Background
Physical activity, sufficient sleep, and limiting sedentary time may improve cancer survivorship.

Methods
Utilizing US nationally representative samples from the National Health Interview Survey 1997-2018 and the National Health and Nutrition Examination Survey 2007-2018, this study investigated the trends of meeting physical activity guidelines, insufficient sleep duration, and sitting time in US cancer survivors (n = 58 527) and noncancer adults (n = 640 109).

Results
From 1997 to 2018, the prevalence of meeting physical activity guidelines was consistently lower in cancer survivors than in noncancer adults. Among cancer survivors, the prevalence of meeting physical activity guidelines increased from 34.9% (95% confidence interval [CI] = 33.1% to 36.8%) to 46.5% (95% CI = 45.0% to 48.1%) for aerobic (≥150 minutes per week at moderate intensity or 75 minutes per week at vigorous intensity), from 13.9% (95% CI = 12.8% to 15.1%) to 23.1% (95% CI = 21.8% to 24.4%) for muscle strengthening (≥2 days per week) activities, and from 9.5% (95% CI = 8.4% to 10.7%) to 17.9% (95% CI = 16.7% to 19.1%) for both combined (all Ptrend &lt; .001). From 2004 to 2018, the prevalence of insufficient sleep duration (&lt;7 hours per day) increased from 28.4% (95% CI = 26.3% to 30.5%) to 30.8% (95% CI = 29.3% to 32.2%) (Ptrend = .004). Daily sitting time increased from 6.09 hours per day (95% CI = 5.71 to 6.46 hours per day) in 2007-2008 to 7.36 hours per day (95% CI = 7.05 to 7.68 hours per day) in 2013-2014 and attenuated to 6.20 hours per day (95% CI = 5.74 to 6.65 hours per day) in 2017-2018. The pattern of physical activity, sleep, and sitting time varied by sex, race and ethnicity, body mass index, cancer type, and time since cancer diagnosis.

Conclusions
More than half of US cancer survivors did not meet physical activity guidelines, and a large proportion had insufficient sleep duration and prolonged sitting time.
Hot, hotter, hottest?
Authors: Prats Jordi 
Published: August 2023

Abstract
Extreme value theory (EVT) uses the historical record to predict which parts of the world can expect exceptional heatwaves, so that policy-makers can try to mitigate the impact. But as statistically implausible heatwaves hit countries across the world, asks Jordi Prats, can EVT still be relied on to keep people safe?