bluuebunny/crossref_metadata_2025_split huggingface dataset:
A slc38a8 mouse model of FHONDA syndrome faithfully recapitulates the visual deficits of albinism without pigmentation defects
Authors: Guardia Ana , Fernández Almudena , Seruggia Davide , Chotard Virginie , Sánchez-Castillo Carla , Kutsyr Oksana , Sánchez-Sáez Xavier , Zurita Esther , Cantero Marta , Rebsam Alexandra , Cuenca Nicolás , Montoliu Lluís 
Published: August 2023

SummaryPurposeWe aimed to generate and phenotype a mouse model of FHONDA (Foveal Hypoplasia, Optic Nerve Decussation Defects, and Anterior Segment Dysgenesis), a rare disease associated with mutations inSLC38A8that causes severe visual alterations similar to albinism without affecting pigmentation.MethodsThe FHONDA mouse model was generated with CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats)/Cas9 technology using an RNA guide targeting theScl38a8murine locus. The resulting mice were backcrossed to C57BL/6J. Melanin content was measured using spectrophotometry. Retinal cell architecture was analyzed through light and electron microscopy. Retinal projections to the brain were evaluated with anterograde labelling in embryos and adults. Visual function was assessed by electroretinography (ERG) and the optomotor test (OT).ResultsFrom numerousSlc38a8mouse mutant alleles generated, we selected one that encodes a truncated protein (p.196Pro*, equivalent to p.199Pro* in the human protein) closely resembling a mutant allele described in patients (p.200Gln*).Slc38a8mutant mice exhibit wild-type eye and coat pigmentation with comparable melanin contents. Subcellular abnormalities were observed in retinal pigment epithelium cells ofSlc38a8mutant mice. Anterograde labelling experiments of retinal projections in embryos and adults showed a reduction of ipsilateral fibers. Functional visual analyses revealed a decreased ERG response in scotopic conditions and a reduction of visual acuity in mutant mice measured by OT.ConclusionsSlc38a8mutant mice recapitulate the phenotype of FHONDA patients concerning their normal pigmentation and their abnormal visual system, as observed in all types of albinism. These mice will be helpful in better understanding the pathophysiology of this genetic condition.
Formation of templated inclusions in a forebrain α-synuclein mouse model is independent of LRRK2
Authors: Dues Dylan J. , Ma Yue , Nguyen An Phu Tran , Offerman Alina V. , Beddows Ian , Moore Darren J. 
Published: August 2023

AbstractLeucine-rich repeat kinase 2 (LRRK2) and α-synuclein share enigmatic roles in the pathobiology of Parkinson’s disease (PD).LRRK2mutations are a common genetic cause of PD which, in addition to neurodegeneration, often present with abnormal deposits of α-synuclein in the form of Lewy-related pathology. As Lewy-related pathology is a prominent neuropathologic finding in sporadic PD, the relationship between LRRK2 and α-synuclein has garnered considerable interest. However, whether and how LRRK2 might influence the accumulation of Lewy-related pathology remains poorly understood. Through stereotactic injection of mouse α-synuclein pre-formed fibrils (PFF), we modeled the spread of Lewy-related pathology within forebrain regions where LRRK2 is most highly expressed. The impact ofLRRK2genotype on the formation of α-synuclein inclusions was evaluated at 1-month post-injection. Neither deletion ofLRRK2nor G2019S LRRK2 knockin appreciably altered the burden of α- synuclein pathology at this early timepoint. These observations fail to provide support for a robust pathophysiologic interaction between LRRK2 and α-synuclein in the forebrainin vivo. There was, however, a modest reduction in microglial activation induced by PFF delivery in the hippocampus ofLRRK2knockout mice, suggesting that LRRK2 may contribute to α-synuclein-induced neuroinflammation. Collectively, our data indicate that the pathological accumulation of α-synuclein in the mouse forebrain is largely independent of LRRK2.HighlightsAdult mice accumulate α-synuclein pathology in the hippocampus and cortex following stereotactic injection with α-synuclein PFFs, with negligible influence ofLRRK2genotype.Hippocampal and cortical α-synuclein pathology elicits the concomitant accrual of phosphorylated tau, reactive astrogliosis, and microglial activation.Absence of endogenousLRRK2attenuates microglial activation in the dorsal hippocampus induced by PFFs, but not in the entorhinal cortex.Accumulation of α-synuclein inclusions and related neuropathologic changes were strongly associated across the hippocampal dorsal-ventral axis, regardless ofLRRK2genotype.
AP2A1 is upregulated upon replicative senescence of human fibroblasts to strengthen focal adhesions via integrin β1 translocation along stress fibers
Authors: Chantachotikul Pirawan , Liu Shiyou , Furukawa Kana , Deguchi Shinji 
Published: August 2023

AbstractAging proceeds with accumulation of senescent cells in multiple organs. Senescent cells become large in size compared to young cells, which promotes further senescence and age-related diseases. Currently, the molecular mechanism behind the maintenance of such huge cell architecture undergoing senescence remains poorly understood. Here we focus on reorganization of actin stress fibers induced upon replicative senescence of human fibroblasts, typically used as a senescent cell model. We identified, together with our previous proteomic study, that AP2A1 (alpha 1 adaptin subunit of the adaptor protein 2) is upregulated in senescent cells along the length of stress fibers, which are enlarged following the increase in the whole cell size. We then revealed that knockdown of AP2A1 in senescent cells suppresses key senescence-associated phenotypes, which include decreased cell area and lowered expression of major senescence markers. Meanwhile, AP2A1 overexpression in young cells induced the opposite effects that rather advance senescence, suggesting that AP2A1 may be used as a senescence marker. We found that AP2A1 is colocalized with integrin β1, and both of them move linearly along stress fibers. We further observed that focal adhesions are enlarged in senescent cells to reinforce cell adhesions to the substrate. These results suggest that senescent cells maintain their large size by strengthening the anchorage to the substrate by supplying integrin β1 via translocation along stress fibers. This mechanism may work efficiently in senescent cells, compared with a case relying on random diffusion of integrin β1, given the enlarged cell size and resulting increase in travel time and distance for endocytosed vesicle transportation.
From brick and mortar to click and order: consumers' online food delivery service perceptions post-pandemic
Authors: Sharma Shavneet , Devi Kritika , Naidu Samantha , Greig Tuma , Singh Gurmeet , Slack Neale 
Published: August 2023

PurposeThis study explores consumers' intentions to utilize online food delivery services (OFDS) in a shared economy beyond the COVID-19 pandemic, employing the protection motivation theory (PMT) as the underlying framework.Design/methodology/approachUtilizing a random sampling technique, a quantitative approach was employed to gather responses from 347 Australian consumers. The proposed model was tested through covariance-based structural equation modelling.FindingsThe findings of this study demonstrate significant positive relationships between restaurant credibility, food quality, e-service quality, price, online food delivery applications, consumer e-satisfaction and e-loyalty. It reveals that consumers satisfied with OFDS may continue exhibiting e-loyalty intentions in a shared economy beyond COVID-19. The relationship between consumer e-satisfaction and e-loyalty intention is moderated by consumer-perceived COVID-19 risk.Practical implicationsThis study offers practical implications for online food delivery providers, restaurants, regulators, application developers and policymakers. These implications aim to enhance the e-service quality, price value, usefulness and security of OFDS, along with strategies to improve the online food delivery application.Originality/valueThis study contributes to the existing body of knowledge by examining a unique selection of antecedents, including the OFDS app, to determine consumer e-satisfaction and e-loyalty in the context of a shared economy beyond COVID-19. The utilization of the OFDS app as a second-order construct adds a meaningful contribution to the OFDS literature. Furthermore, this study investigates and contributes to the limited understanding of the moderation effect of consumer-perceived COVID-19 risk on consumer e-satisfaction and their intended continued use of OFDS.
The effect of income and expense shifting on the corporate income tax evasion
Authors: Kołodziej Sabina , Maruszewska Ewa Wanda , Niesiobędzka Małgorzata 
Published: August 2023

PurposeThis paper aims to present a study on the effect of income and expense shifting on the corporate income tax evasion – an example of intentional noncompliance practiced by tax agents. The authors expected that the tool used would differentiate the extent of understatement of tax liability.Design/methodology/approachTwo experiments were conducted in which young (N = 62) and experienced (N = 68) tax agents read a scenario placing them in a position of an employee responsible for tax planning and calculations of tax liabilities. The respondents’ task was to decide about the extent of the tax liability understatement using income or expense shifting.FindingsResearch demonstrated significantly higher extent of corporate income tax understatement when using income shifting compared to expense shifting in case of experienced tax agents (Study 2) and on tendency level among young tax agents (Study 1).Research limitations/implicationsResults of the studies might be of interest to managers paying attention to tax procedures within the company, governmental agencies investigating corporate tax evasion, as well as educators responsible for tax agents’ initial training and lifelong learning.Originality/valueThis study concentrates on tax agents who are employed in companies and corporate income tax evasion, which has not been analyzed in the literature so far.
Optimization of V-shaped regulating ball valve profile based on proxy optimization algorithm
Authors: Li Shuxun , Yu Mengyao , Wu Hanlin , Hu Yinggang , Ma Tingqian , Liu Bincai 
Published: August 2023

PurposeThe purpose of this study is to address the issue that the traditional V-shaped ball valve profile shape is limiting the flow control characteristics in a series structure and to optimize the design profile by proposing an open-hole profile.Design/methodology/approachThis paper proposes a Gaussian process regression surrogate model based on the genetic algorithm optimization of swarm intelligence, combined with the Expected Improvement point addition criterion, to optimize and correct the design profile. The flow regulation performance of the optimized V-shaped regulating ball valve is verified through a combination of numerical simulation and experiment.FindingsThe results demonstrate that the optimized V-shaped regulating ball valve has higher flow regulation accuracy and a more stable flow regulation process. After optimization, the flow characteristic curve of the spool is closer to the ideal equal percentage characteristic. The simulation results of the flow field are consistent with the experimental results.Originality/valueThe proposed method significantly reduces the optimization time, has higher efficiency and solves the problem that traditional optimization methods struggle with, which is ensuring optimal flow regulation performance. Compared to the traditional trial-and-error optimization method, the proposed method is more effective. The feasibility of the method is supported by experimental results.
Numerical analysis for tunnelling-induced stress and plastic evolution causing instability of multilayered surrounding rock by varying three-dimensional in situ stresses
Authors: Wang Yongliang , Feng Ruiguang , Li Dongping , Peng Ruidong 
Published: August 2023

PurposeThe induction of geological disasters is predominantly influenced by the dynamic evolution of the stress and plastic zones of the multilayer rock formations surrounding deep-rock roadways, and the behaviours and mechanisms of high in situ stress are key scientific issues related to deep-resource exploitation. The stress environment of deep resources is more complex owing to the influence of several geological factors, such as tectonic movements and landforms. Therefore, in practical engineering, the in situ stress field is in a complex anisotropic three-dimensional state, which may change the deformation and failure law of the surrounding rock. The purpose of this study is to investigate the tunnelling-induced stress and plastic evolution causing instability of multilayered surrounding rock by varying three-dimensional in situ stresses.Design/methodology/approachBased on data from the Yangquan Coal Mine, China, a finite difference model was established, and the elastic-plastic constitutive model and element deletion technology designed in the study were analysed. Gradual tunnelling along the roof and floor of the coal seam was used in the model, which predicted the impact tendency, and compared the results with the impact tendency report to verify the validity of the model. The evolutions of the stress field and plastic zone of the coal roadway in different stress fields were studied by modifying the maximum horizontal in situ stress, minimum horizontal in situ stress and lateral pressure coefficient.FindingsThe results shown that the in situ stress influenced the stress distribution and plastic zone of the surrounding rock. With an increase in the minimum horizontal in situ stress, the vertical in situ stress release area of the roof surrounding rock slowly decreased; the area of vertical in situ stress concentration area of the deep surrounding rock on roadway sides decreased, increased and decreased by turn; the area of roof now-shear failure area first increased and then decreased. With an increase in the lateral pressure coefficient, the area of the horizontal in situ stress release area of the surrounding rock increased; the area of vertical in situ stress release area of the roof and floor surrounding rock first decreased and then increased; the area of deep stress concentration area of roadway sides decreased; and the plastic area of the surrounding rock and the area of now-shear failure first decreased and then increased.Originality/valueThe results obtained in this study are based on actual cases and reveal the evolution law of the disturbing stress and plastic zone of multilayer surrounding rock caused by three-dimensional in situ stress during the excavation of deep rock roadways, which can provide a practical reference for the extraction of deep resources.
The motivational influence of collective efficacy and charged behavior and the moderating effect of risk-taking propensity on new product development team innovativeness
Authors: Rodriguez Carlos M. 
Published: August 2023

PurposeThis study examines the motivational processes of charged behavior and collective efficacy driving interdependence and agency in new product development (NPD) teams and the moderating impact of team risk-taking propensity as affective, cognitive and behavioral social processes support team innovation.Design/methodology/approachData were collected from 92 NPD teams engaged in B2C and B2B product and service development. Mediating and moderating effects are examined using partial least squares structural equation modeling, referencing social cognitive and collective agency theories as the research framework.FindingsThe analysis validates collective self-efficacy and charged behavior as interdependent motivational–affective processes that align cognitive resources and govern team effort toward innovativeness. Teams' risk-taking propensity regulates behavior, and collective efficacy facilitates self-regulated motivational engagement. Charged behavior cultivates the emotional contagion, team identification, cohesion and adaptation required for team functioning. Team potency fosters cohesiveness, while team learning improves adaptability along the innovation journey. The resulting theory asserts that motivational drivers enhance the interplay between cognitive and behavioral processes.Practical implicationsManagers should consider NPD teams as social systems with a capacity for collective agency nurtured through interdependence, which requires collective efficacy and shared competencies to generate motivational purpose and innovativeness. Managers must remain mindful of teams' risk tolerance as regulating the impact of motivational factors on innovativeness.Originality/valueThis study contributes to research on the motivational–affective drivers of NPD charged behavior and collective efficacy as complementary to cognitive and behavioral processes sustaining team innovativeness.
Role of passion in entrepreneurial responses to crises on social media platforms
Authors: Boussema Samira 
Published: August 2023

PurposeThis study examines the role of passion in gender-specific entrepreneurial responses in times of crises and how passion manifests itself in a digital environment. Entrepreneurial passion feeds energy, tenacity, self-confidence and momentum, creating added value for a given economy.Design/methodology/approachA qualitative approach was used to conduct semi-structured interviews. To reach the research objectives, the author examined a sample of 22 female entrepreneurs and 19 male entrepreneurs in a comparative design.FindingsThe results obtained through a qualitative study prove that entrepreneurial passion is a driver of self-confidence for entrepreneurs. Moreover, the nature of entrepreneurial passion differs across gender in times of crises. Indeed, female entrepreneurs try to take their lives into their own hands by creating their own destinies. They have seized opportunities arising from purely technological progress to create their own businesses on social networks and solve the unemployment problem. Meanwhile, male entrepreneurs pursue opportunities based on market supply and demand to increase their market share and face a crisis.Practical implicationsThese findings offer novel insights into research on social media entrepreneurs. This study could help new entrepreneurs highlight their abilities, particularly those that are most salient and central.Originality/valueThis study adds a new dimension to the literature on the role and nature of entrepreneurial passion in times of crises.
Drivers of strengthening university–industry collaboration: implications for favorable outcomes
Authors: Kamal Md Aktar , Guha Souman , Begum Noor Nahar , Taher Md Abu 
Published: August 2023

PurposeThe purpose of the study was to examine the factors that are important for strengthening university–industry collaboration (UIC). This study also investigates the outcome of UIC in the light of creativity, skill, knowledge, and research work.Design/methodology/approachA survey method has been used to collect data for the study. This study applied a purposive judgmental sampling technique where particular types of respondents like university faculty members and the top officials of the organizations were selected who are knowledgeable and can provide the desired information. The current study used the structural equation modeling method to analyze the data. In the first stage, this research assessed the demographic factors of the respondent. Then this study conducts confirmatory factors analysis and convergent and discriminant validity and reliability test. Finally, the hypotheses are tested by using nonparametric.FindingsThis study finds that knowledge transfer mechanism, governmental factors, organizational design factors, technology transfer and the collaborative network has a significant impact on strengthening UIC, which ultimately facilitates creativity, knowledge creation, skills development and supply of graduate according to the requirement of the industry, good research work.Originality/valueThe current study identified some important determinant that has a substantial influence on strengthening UIC. According to the study organizational design, government, technology, collaborative network and mechanism for knowledge transfer play very crucial roles in strengthening collaboration that ultimately increases the creativity, skills, knowledge and research capability of graduates.
Does CEO celebrity affect IPO underpricing? Evidence from the strategic emerging industries in China
Authors: Yang Shuai , Zhao Yu , Wu Chao 
Published: August 2023

PurposeThe interaction between evaluators is underestimated in legitimacy literature. This study aims to examine the impact of CEO celebrity on initial public offerings (IPOs) underpricing in Strategic Emerging Industries (SEIs). Based on legitimacy and limited attention effect, this study introduces a new antecedent to the asset pricing literature under a particular sample.Design/methodology/approachThis paper illustrates how CEO celebrity promotes IPO underpricing by enhancing the legitimacy and then explores how the CEO characteristics can moderate this relationship. Using 1,128 IPO companies in China SEIs from 2010 to 2019, cross-section data is used to build a multiple linear regression model to test the hypotheses.FindingsThe result indicates that CEO celebrity is positively related to IPO underpricing. Founder CEO and CEO duality amplify the relationship. Further analysis shows that the relationship between CEO celebrity and IPO underpricing is more pronounced in firms with high Baidu search and low market sentiment.Originality/valueThis study provides insights into how CEO celebrity as notable internal information shapes the formation of investors' preliminary impressions of firms. The evidence consists of legitimacy and limited attention perspective by showing how investors favor, follow and hype the stocks with celebrity CEOs. The results extend the knowledge about how CEO characteristics influence information frictions in asset pricing during IPO.
Satellite imagery and machine learning for channel member selection
Authors: Brei Vinicius Andrade , Rech Nicole , Bozkaya Burçin , Balcisoy Selim , Pentland Alex Paul , Silveira Netto Carla Freitas 
Published: August 2023

PurposeThis study aims to propose a new method to predict retail store performance using publicly available satellite imagery data and machine learning (ML) algorithms. The goal is to provide manufacturers and other practitioners with a more accurate and objective way to assess potential channel members and mitigate information asymmetry in channel selection and negotiation.Design/methodology/approachThe authors developed an open-source approach using publicly available Google satellite imagery and ML algorithms. A computer vision algorithm was used to count cars in store parking lots, and the data were processed with a CNN. Linear regression and various ML algorithms were used to estimate the relationship between parked cars and sales.FindingsThe relationship between parked cars and sales was nonlinear and dependent on the type of channel member. The best model, a Stacked Ensemble, showed that parking lot occupancy could accurately predict channel member performance.Research limitations/implicationsThe proposed approach offers manufacturers a low-cost and scalable solution to improve their channel member selection and performance assessment process. Using satellite imagery data can help balance the marketing channel planning process by reducing information asymmetry and providing a more objective way to assess potential partners.Originality/valueThis research is unique in proposing a method based on publicly available satellite imagery data to assess and predict channel member performance instead of forward-looking sales at the firm and industry levels like previous studies.