bluuebunny/crossref_metadata_2025_split huggingface dataset:
Simple and low‐cost antibiotic susceptibility testing for Mycobacterium tuberculosis using screen‐printed electrodes
Authors: Ghorbanpoor Hamed , Akcakoca Iremnur , Norouz Dizaji Araz , Butterworth Adrian , Corrigan Damion , Kocagoz Tanil , Ebrahimi Aliakbar , Avci Huseyin , Dogan Guzel Fatma 
Published: February 2023

AbstractOne quarter of the global population is thought to be latently infected by Mycobacterium tuberculosis (TB) with it estimated that 1 in 10 of those people will go on to develop active disease. Due to the fact that M. tuberculosis (TB) is a disease most often associated with low‐ and middle‐income countries, it is critical that low‐cost and easy‐to‐use technological solutions are developed, which can have a direct impact on diagnosis and prescribing practice for TB. One area where intervention could be particularly useful is antibiotic susceptibility testing (AST). This work presents a low‐cost, simple‐to‐use AST sensor that can detect drug susceptibility on the basis of changing RNA abundance for the typically slow‐growing M. tuberculosis (TB) pathogen in 96 h using screen‐printed electrodes and standard molecular biology laboratory reactionware. In order to find out the sensitivity of applied sensor platform, a different concentration (108–103 CFU/mL) of M. tuberculosis was performed, and limit of detection and limit of quantitation were calculated as 103.82 and 1011.59 CFU/mL, respectively. The results display that it was possible to detect TB sequences and distinguish antibiotic‐treated cells from untreated cells with a label‐free molecular detection. These findings pave the way for the development of a comprehensive, low‐cost, and simple‐to‐use AST system for prescribing in TB and multidrug‐resistant tuberculosis.
Embryo‐fetal safety evaluation of ondansetron in rats
Authors: Reis Ana Carolina Casali , Jorge Bárbara Campos , da Silva Moreira Suyane , Stein Júlia , Perdão Carolina Barizan , de Matos Manoel Beatriz , Arena Arielle Cristina 
Published: February 2023

AbstractBackgroundOndansetron is a 5HT3 receptor antagonist, used to mitigate the effects of nausea and vomiting after chemotherapy or surgery. Since nausea and vomiting are common experiences during the first trimester of pregnancy, this antiemetic has been the main drug used during this period.MethodsTo evaluate the effects of ondansetron on the embryo‐fetal development, which are still very contradictory, pregnant rats were exposed to therapeutic doses of ondansetron (1.7 or 2.5 mg/kg) daily, from gestational day (GD) 6 to 15.ResultsNo clinical signs of toxicity were observed in dams during the treatment. Although the hemato‐biochemical parameters were similar among the groups, histological changes, as well as a reduction in the weight of kidney were found in the treated dams. After fetal examination, no visceral and skeletal abnormalities were observed in treated fetuses.ConclusionIn conclusion, therapeutic doses of ondansetron have low teratogenic potential in rats. These data provide important information about the drug safety during pregnancy.
Thermoluminescence in pumice stone collected from the Mediterranean coast
Authors: Toktamış Dilek 
Published: February 2023

AbstractPumice is a low‐density, light‐coloured volcanic rock (igneous rock) formed when the magma from a volcanic eruption is suddenly cooled. It has a porous structure and can be different colours and have different densities. Pumice stone, unlike other rock, does not sink in water because it has a low density. In the present study, the thermoluminescent (TL) dosimetric characteristics, such as the dose–response, TL signal fading as a function of storage time, heating rate, and reusability of pumice collected on the Mediterranean coast were investigated. From this study, it was concluded that the pumice stone showed TL properties with a TL glow curve including an obviously wide peak at ∼200°C. A wide linear dose–response region up to 144 Gy, low fading of the TL signal when it was kept in a dark room for a long time, and poor reusability properties were observed for dosimetry use.
Effects of predictive nursing intervention on cognitive impairment and neurological function in ischemic stroke patients
Authors: Xue Lianyu , Deng Jiangshan , Zhu Lingyan , Shen Feifei , Wei Jiewei , Wang Lihui , Chen Qinqin , Wang Lan 
Published: February 2023

AbstractBackgroundIschemic stroke is a clinical emergency caused by insufficient intracranial blood supply, which eventually leads to brain tissue necrosis and neurological impairment. Predictive nursing intervention has achieved impressive success in the nursing of multiple surgeries. However, the role of predictive nursing intervention in the care of patients with ischemic stroke remains unclear.MethodsThis study was a randomized controlled trial. Based on the inclusion and exclusion criteria, 126 patients were randomly assigned into two groups, namely the control group and the predictive nursing intervention group. Both groups were treated with thrombolytic therapy with alteplase. The patients in the control group were given routine nursing intervention and the predictive nursing intervention group received additional predictive care. Neurologic functions and cognitive impairment were evaluated by National Institutes of Health Stroke Scale (NIHSS), Fugl‐Meyer assessment (FMA), Montreal cognitive assessment (MoCA), and mini‐mental state examination (MMSE) scales, respectively. Door‐to‐Needle Times, venous thromboembolism (VTE)‐related parameters, and complications were recorded.ResultsPredictive nursing intervention significantly shortened the Door‐to‐Needle Times and enhanced the peak/average femoral venous blood flow and femoral venous diameter. In addition, predictive nursing intervention improved the NIHSS, FMA, MMSE, and MoCA scores and remarkably reduced the recurrence of ischemic stroke, deep vein thrombosis and gingival bleeding.ConclusionPredictive nursing intervention is beneficial to improve the effects of thrombolytic therapy in patients with ischemic stroke, which improves the neurological, cognitive and motor functions of patients, and reduces the occurrence of complications, suggesting an important clinical application value.
Sustainable mobility choices: Exploring the impact of consumers' values, attitudes, perceived behavioural control and subjective norms on the likelihood to choose sustainable mobility options
Authors: Nogueira Mafalda , Dias Francisca , Santos Vera 
Published: February 2023

AbstractThis study explores consumers' behaviour regarding sustainable mobility options. A mixed‐methods approach was adopted to examine the impact of bio‐altruistic values, egoistic values, environmental attitudes, attitudes regarding sustainable mobility, perceived behavioural control and subjective norms on the likelihood of choosing sustainable mobility options. To collect the data, an online survey was applied to a sample of 645 Portuguese consumers, which included one single non‐mandatory open‐ended question to understand which factors could contribute to increase respondents' likelihood to choose more sustainable transport modes. As most studies in the mobility field are quantitative, combining both quantitative and qualitative data provided a greater in‐depth analysis with richer findings. Firstly, findings revealed that attitudes towards sustainable mobility, subjective norms, perceived behaviour control, and egoistic values influence the likelihood of choosing sustainable mobility options. However, environmental attitudes, altruistic and biospheric values do not impact this choice. Secondly, consumers highlight that the existence of a broad public transport network, safe bicycle lanes, and accessible prices would contribute to their likelihood of adopting more sustainable mobility behaviours. The content analysis of 402 answers allowed to discover new dimensions that influence sustainable mobility behaviour, such as safety, respect towards bicycle users, mindset adjustment, willpower, and especially physical efforts associated with long distances, bad weather, orography, and the difficulty to transport materials or other people. Based on both quantitative and qualitative findings, the paper provides key recommendations for politicians, urban planners, and transport organisations, that according to respondents, would contribute to increase their use of sustainable mobility.
Effect of cricket protein powders on dough functionality and bread quality
Authors: Perez‐Fajardo Mayra , Bean Scott R. , Dogan Hulya 
Published: February 2023

AbstractBackground and ObjectivesThe enrichment of foods with insect proteins has gained interest; however, more research is needed to understand how different insect protein functionalities impact food systems. The objective of this study was to understand the effect of cricket protein powder on mixing, pasting, and dough development characteristics of bread dough and on the end‐product characteristics of bread.FindingsTwo different cricket protein powders, GrioPro (G) and Entomo Farms €, were tested. MixoLab constant and optimized water absorption protocols were used to study the effect of cricket protein powder replacement on dough development. Dough extensibility was tested using the Kieffer Rig protocol. Breads were baked with 5%, 10%, or 20% replacement levels of cricket protein powder. Loaf volume was measured, and bread slices underwent texture profile analysis (TPA) at 0, 1, 3, and 7 days. In general, the incorporation of powders G and E led to two opposite effects. Use of powder G led to an increased stability and significantly higher C1 torque (20% level) while powder E led to softer doughs with a decreased stability at the 20% replacement level and no significant difference in water absorption.ConclusionsExtensibility and loaf volume significantly decreased as the replacement level increased for all treatments. TPA results showed a significant increase in hardness at higher replacement levels with G being harder than E. It is feasible to enrich bread with cricket protein powders at low incorporation levels.Significance and NoveltyResults show how differing functional properties of cricket protein powders impact their interactions within the gluten network and in turn affect the quality of bread produced.
Effect of infrared heating on the nutritional properties of yellow pea and green lentil flours
Authors: Laing Emma , Stone Andrea K. , Shi Dai , Pickard Mark , House James D. , Wang Ning , Nickerson Michael T. 
Published: February 2023

AbstractBackground and ObjectivesTo enhance the utilization of pulse ingredients, greater knowledge of the effect of infrared (IR) processing on protein and starch nutrition is needed. The current study investigated the use of tempering (20% vs. 30% moisture) with IR heating (120°C vs. 140°C) to improve the nutritional value of two commercially important pulses: green lentils and yellow peas.FindingsProximate composition remained mostly unchanged after IR heating for both pulse types. The protein's secondary structure transitioned to a state with a higher amount of random coils as IR processing conditions intensified (increase in moisture and temperature). In vitro protein digestibility (IVPD) increased from 73% to 78%−82% for green lentil and 78% to 81%−85% for yellow pea, depending on IR processing treatment. Tryptophan was the limiting amino acid in all samples. The IVPD corrected amino acid scores were not significantly altered by IR processing. The content of rapidly (RDS) and slowly (SDS) digestible starches increased, whereas that of resistance starch declined with IR processing.ConclusionsThe combined effect of tempering moisture and IR heat as a premilling treatment changed the protein secondary structure but did not improve the overall protein quality of the pulses. Starch digestibility was improved with IR processing.Significance and NoveltyEmploying tempering and IR heating techniques on pulses may be useful in food and feed applications where improved starch digestibility is desired.
Unravelling the Effect of Alkali Metal Deposition on Co3O4 for Catalytic Decomposition of N2O
Authors: Sun Jinru , Song Aolei , Tian Yu , Zhan Hua , Deng Jianlin , Wang Hong , Ke Ming 
Published: February 2023

AbstractIn this paper, the effect of alkali metal deposition on the activity of x‐Co3O4 (x=Na, K, Rb, Cs) catalysts for the N2O catalytic decomposition was systematically explored by DFT calculation and various experimental characterization methods. The results demonstrated that the deposition of alkali metal Na improved the activity of the Na‐Co3O4 catalyst for N2O catalytic decomposition at low temperatures. By contrast, the deposition of other alkali metals (K, Rb and Cs) suppressed the catalytic decomposition of N2O perceptibly. Analysis of the results of XRD, FT‐IR, SEM, TEM, XPS, O2‐TPD and H2‐TPR characterization of x‐Co3O4 catalysts showed that the deposition of alkali metals did not affect the morphology and structure of the catalysts. However, it affected the chemical environment around the Co ion in the x‐Co3O4 catalyst, where the deposition of alkali metal Na not only facilitated the reduction of Co3+ to Co2+, but also promoted the surface decomposition step of N2O (i. e., the oxidation of Co2+ to Co3+ by giving electrons) at low temperatures, indicating that alkali metal Na improved the electron donating capability of the catalyst active center and enhanced the performance of the Na‐Co3O4 catalyst for the DCD of N2O at low temperatures. The deposition of other alkali metals did not show the same results as that of alkali metal Na, indicating that the promotion of different alkali metals was different and would directly affect the catalytic effect. At low temperatures, the catalytic activity of catalysts deposited with the alkali metal Na was greater than that of catalysts deposited with other alkali metals.
Precise Control of Dissipative Self‐assembly by Light and Electricity
Authors: Chen Chunfeng , Guan Zhibin 
Published: February 2023

AbstractNature‐inspired synthetic dissipative self‐assemblies have attracted much attention recently. However, it remains a major challenge to achieve precise control over dissipative supramolecular assembly structures and functions of self‐contained systems. Here we combine light and electricity as two clean, and spatiotemporally addressable fuels to provide precise control over the morphology for dissipative self‐assembly of a perylene bisimide glycine (PBIg) building block in a self‐contained solution. In this design, electrochemical oxidation provides the positive fuel to activate PBIg self‐assembly while photoreduction supplies the negative fuel to deactivate the system for disassembly. Through programming the two counteracting fuels, we demonstrated the control of PBIg self‐assembly into a variety of assembly morphologies in a self‐contained system. In addition, by exerting light and electrical dual fuels simultaneously, we could create an active homeostasis exhibiting dynamic instability, leading to morphological change to asymmetric assemblies with curvatures. Such precise control over self‐assembly of self‐contained systems may find future applications in programming complex active materials as well as formulating pharmaceutical reagents with desired morphologies.
Highly selective extraction of aromatics from aliphatics using an N‐methylpyrrolidone‐based protic ionic liquid
Authors: Yu Hui , Geng Chuanqi , Li Xinyu , Wu Xiaojia , Tian Shichao , Zhou Zhiyong , Ren Zhongqi 
Published: February 2023

AbstractThe efficient and sustainable separation of aromatics from aliphatics is an ongoing challenge in chemical engineering. In this work, the protic ionic liquid N‐methylpyrrolidonium methanesulphonate ([NMP][MSA]) was used to separate toluene from n‐heptane or cyclohexane. Nuclear magnetic resonance and Fourier‐transform infrared spectroscopy were used to demonstrate the successfully synthesis of [NMP][MSA]. Density functional theory (DFT) calculations were used to confirm that the interaction of [NMP][MSA] with toluene would be stronger than those with n‐heptane and cyclohexane, with electrostatic forces and dispersion playing the primary roles and induction playing a minor role. Furthermore, independent gradient model (IGM) analysis demonstrated that van der Waals forces make a major contribution to the stability of [NMP][MSA]–toluene. For a 10 mol% toluene/n‐heptane system, the toluene distribution coefficient and selectivity were found to be 0.35 and >70, respectively, while the corresponding values were 0.40 and 27 for a 10 mol% toluene/cyclohexane system. Changing the extraction temperature seemed to have little effect on separation performance. The effects of solvent dosage, initial concentration, and extractant recycling were also investigated. Overall, our results show that [NMP][MSA] has potential for industrial use.
Simultaneous removal of anionic and cationic dyes from wastewater with biosorbents from banana peels
Authors: Stavrinou Anastasia , Aggelopoulos Christos A. , Tsakiroglou Christos D. 
Published: February 2023

AbstractUntreated banana peel (BP) is able to adsorb efficiently cationic dyes like methylene blue (MB) but weakly anionic dyes like orange G (OG). One way to enhance the sorption capacity for both dyes is to convert BP into activated carbon (BPAC) after thermochemical treatment. Among the various BPACs tested, the highest sorption capacity for both dyes was achieved when BP was modified with NaOH and pyrolyzed at 700°C (BPAC‐NaOH‐700). The pore structure of adsorbents was analyzed with scanning‐electron‐microscopy (SEM), nitrogen sorption isotherms, and mercury intrusion porosimetry (MIP), and the meso‐ and micro‐pore size distribution of BPAC‐NaOH‐700 was the narrowest one with the smallest mean value and the highest specific surface area. Equilibrium tests in batch mode were fitted with the extended Langmuir and Freundlich isotherms and used to estimate thermodynamic properties. The MB and OG sorption dynamics onto BPAC‐NaOH‐700 was approximated with the multi‐compartment model, and the external and internal mass‐transfer coefficients were estimated. The maximum sorption capacity of BPAC‐NaOH‐700 was found to be equal to 323 mg/g for MB and 76 mg/g for OG, both higher than the corresponding values for BP, and fully consistent with the high values of its surface area ( = 530 m2/g) and total pore volume ( = 1.81 cm3/g). The synergistic interaction for the sorption of both dyes onto BPAC‐NaOH‐700 was associated with a push‐pull mechanism, while the selective sorption of MB onto BPAC‐NaOH‐700 was attributed to the very slow rates of OG pore and surface diffusion in meso‐ and micro‐porosity, respectively. The adsorption was exothermic for BP, and endothermic for OG.
Comparative Performance Assessment of Adsorbents for CO2 Capture via Physisorption in a Diesel Engine Exhaust
Authors: Ravi Maniarasu 
Published: February 2023

AbstractThe atmospheric carbon dioxide (CO2) concentration has been consistently increasing each year throughout the world. Internal combustion (IC) engines are significant contributors to CO2 emissions. This study explores the possibility of employing effective biomass‐based adsorbents to mitigate CO2 from a diesel engine exhaust. As a first step, two distinct agro‐wastes, namely, i) corn cob and ii) sugarcane bagasse, are used to prepare inexpensive and efficient activated carbons. The two main steps in the activated carbon preparation are a) carbonization and b) activation. The derived activated carbons are subjected to discrete analytical techniques to examine their structural and textural characteristics, surface functional groups, and physical, chemical, and adsorptive properties. As a second step, the exhaust treatment chamber unit is filled with the adsorbents one by one and is connected to the exhaust of the constant pressure heat addition engine. A single‐cylinder, four‐stroke, naturally‐aspirated, air‐cooled, direct injection (DI) compression ignition (CI) engine is used in the experimental investigations. The essential findings show that ≈68 and 60% of CO2 emissions are adsorbed in the test engine by utilizing corn cob and sugarcane bagasse adsorbents, respectively. The results show that during the D100 and JME20 operations, the prospective adsorbents can curb more than 40% of overall CO2 emissions.
Genomic and proteomic characterization of Philadelphia‐like B‐lineage acute lymphoblastic leukemia: A report of Indian patients
Authors: Gupta Dikshat Gopal , Varma Neelam , Kumar Ashish , Naseem Shano , Sachdeva Man Updesh Singh , Sreedharanunni Sreejesh , Binota Jogeshwar , Bose Parveen , Khadwal Alka , Malhotra Pankaj , Varma Subhash 
Published: February 2023

AbstractBackgroundThe gold standard for the identification of Philadelphia (Ph)‐like acute lymphoblastic leukemia (ALL) patients is gene expression profiling. Because of its diverse nature, its identification is extremely difficult and expensive. On the genomic and proteomic landscape of Ph‐like ALL patients, there is a paucity of published literature from developing countries.MethodsThe authors used digital barcoded nCounter NanoString gene expression profiling for its detection, followed by molecular and proteomic characterization using fluorescence in situ hybridization and liquid chromatography–tandem mass spectrometry (LC–MS/MS).ResultsThe authors found 32.05% Ph‐like ALL patients and their median age at presentation was considerably higher than Ph‐negative ALL cases (p = .0306). Furthermore, we identified 20% CRLF2 overexpressed cases having 8.33% CRLF2‐IGH translocation with concomitant R683S mutation and 8.33% CRLF2‐P2RY8 translocation. In 80% of CRLF2 downregulated cases, we identified 10% as having JAK2 rearrangement. Minimal residual disease‐positivity was more common in Ph‐like ALL cases (55.55% vs. 25% in Ph‐negative ALL cases). Immunoglobulin J chain (Jchain), small nuclear ribonucleoprotein SmD1 (SNRPD1), immunoglobulin κ constant (IGKC), NADH dehydrogenase (ubiquinone) 1 α subcomplex subunit 2 (NDUFA2), histone H2AX (H2AFX), charged multivesicular body protein 4b (CHMP4B), and carbonyl reductase (NADPH) (CBR1) proteins were identified to be substantially expressed in Ph‐like ALL patients, using LC–MS/MS. Gene enrichment analysis indicated that involvement of spliceosomal mediated messenger RNA splicing pathway and four microRNAs was statistically significant in Ph‐like ALL patients.ConclusionsFor the first time, we have described incidence, molecular, and proteomic characterization of Ph‐like ALL, in developing nations.Plain language summary

In developing countries, detecting Philadelphia (Ph)‐like B‐lineage acute lymphoblastic leukemia is complicated and challenging due to its diverse genetic landscape.

There is no well‐defined and cost‐effective methodology for its detection.

The incidence of this high‐risk subtype is very high in adult cases, and there is an urgent need for its accurate detection.

We have developed an online PHi‐RACE classifier for its rapid detection, followed by delineating the genomic and proteomic landscape of Ph‐like acute lymphoblastic leukemias for the first time in Indian patients.