bluuebunny/crossref_metadata_2025_split huggingface dataset:
A new iron supplement: The chelate of pig skin collagen peptide and Fe2+ can treat iron-deficiency anemia by modulating intestinal flora
Authors: Jiang Shan , Dong Weichao , Zhang Zhen , Xu Jing , Li Haoran , Zhang Jiayu , Dai Long , Wang Shaoping 
Published: December 2022

IntroductionIron deficiency anemia (IDA) is one of the most common nutritional diseases encountered all over the world. Nowadays, oral iron supplementation is still the mainstay of IDA treatment.MethodsIn this study, a new iron nutritional supplement named pig skin collagen peptides ferrous chelates (PSCP-Fe) was prepared, and its structure was characterized by the scanning electron microscopy, sykam amino acid analyzer and Fourier transform infrared spectroscopy (FTIR). The anti-IDA activity of PSCP-Fe was evaluated in low-Fe2+ diet-induced IDA in rats. 16S amplicon sequencing technology was then used to reveal the mechanism of PSCP-Fe against IDA.ResultsThe results of amino acid analysis and FTIR showed that aspartic acid (Asp), arginine (Arg), histidine (His), glutamic acid (Glu), cystine (Cys), and lysine (Lys) residued in PSCP chelated readily with Fe2+ through their functional groups. PSCP-Fe treated reversed the hematology-related indexes, such as red blood cells (RBC), hemoglobin (HGB), hematocrit (HCT), mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH), mean corpuscular hemoglobin concentrate (MCHC), serum ferritin (SF), serum hepcidin (HEPC) and serum transferrin receptor (TFR). And its regulatory action was better than that of FeSO4. Moreover, PSCP-Fe alleviated the hepatocyte apoptosis and necrosis, Fe2+ loss, and injury in IDA rats. In addition, PSCP-Fe could significantly retrace the disturbed profile of gut microbiota in IDA rats (p &lt; 0.05) and significantly up-regulated the relative abundances of nine bacterial genus, including Lactobacillus, Alloprevotella, unclassified_of_Oscillospiraceae, and NK4A214_group (p &lt; 0.05). It could also downgrade the relative abundances of Subdoligranulum and Coriobacteriaceae_UCG-002 (p &lt; 0.05). The results of Spearman’s correlation analysis and distance-based redundancy analysis (db-RDA) revealed that Subdoligranulum and Christensenellaceae_R-7_group may be potential microbial markers for effective PSCP-Fe action in the treatment of IDA.DiscussionOverall, our results elucidate the interactions between gut bacteria and related cytokines and reveal the mechanisms underlying the anti-IDA effect of PSCP-Fe. They will thus provide a theoretical foundation for PSCP-Fe as a new iron nutritional supplement.
Role of cuproptosis-related gene in lung adenocarcinoma
Authors: Liu Yuan , Lin Wei , Yang Ying , Shao JingJing , Zhao Hongyu , Wang Gaoren , Shen Aiguo 
Published: December 2022

BackgroundsLung adenocarcinoma (LUAD) is the most common subtype of lung cancer, which is the leading cause of cancer death. Dysregulation of cell proliferation and death plays a crucial role in the development of LUAD. As of recently, the role of a new form of cell death, cuproptosis, and it has attracted more and more attention. As of yet, it is not clear whether cuproptosis is involved in the progression of LUAD.MethodsAn integrated set of bioinformatics tools was utilized to analyze the expression and prognostic significance of cuproptosis-related genes. Meanwhile, a robust risk signature was developed using machine learning based on prognostic cuproptosis-related genes and explored the value of prognostic cuproptosis-related signature for clinical applications, functional enrichment and immune landscape. Lastly, the dysregulation of the cuproptosis-related genes in LUAD was validated by in vitro experiment.ResultsIn this study, first, cuproptosis-related genes were found to be differentially expressed in LUAD patients of public databases, and nine of them had prognostic value. Next, a cuproptosis-related model with five features (DLTA, MTF1, GLS, PDHB and PDHA1) was constructed to separate the patients into high- and low-risk groups based on median risk score. Internal validation set and external validation set were used for model validation and evaluation. What’s more, Enrichment analysis of differential genes and the WGCNA identified that cuproptosis-related signatures affected tumor prognosis by influencing tumor immunity. Small molecule compounds were predicted based on differential expressed genes to improve poor prognosis in the high-risk group and a nomogram was constructed to further advance clinical applications. In closing, our data showed that FDX1 affected the prognosis of lung cancer by altering the expression of cuproptosis-related signature.ConclusionA new cuproptosis-related signature for survival prediction was constructed and validated by machine learning algorithm and in vitro experiments to reflect tumor immune infiltration in LUAD patients. The purpose of this article was to provide a potential diagnostic and therapeutic strategy for LUAD.
Early mechanisms of neutrophil activation and transmigration in acute lung injury
Authors: Cagle L. A. , Linderholm A. L. , Franzi L. M. , Last J. A. , Simon S. I. , Kenyon N. J. , Harper R. W. 
Published: December 2022

Introduction:Neutrophil transmigration is multifactorial and primarily driven by selectins and β2-integrins (CD11b/CD18), whose expression are dependent on the underlying stimulus. Ventilator-induced lung injury (VILI) results in a predominantly CD18-independent mechanism of neutrophil recruitment, while direct endotoxin-induced lung injury results from a CD18-dependent mechanism. We previously observed that lack of NADPH oxidases DUOX1 and DUOX2 resulted in reduced neutrophil influx in a VILI model of lung injury but had no influence on neutrophil influx after LPS exposure. Based on these observations, we hypothesized that DUOX1/DUOX2 are an important component of CD18-independent mechanisms of neutrophil recruitment in the lung.Methods:We exposedDuoxa−/−(KO) mice andDuoxa+/+(WT) mice to either an intratracheal exposure of lipopolysaccharide (LPS/endotoxin)-or high tidal volume ventilation and compared expression of neutrophil markers between groups. WT mice (129S6/SvEvTac) were obtained from Taconic Biosciences (One Discovery Drive Suite 304; Rensselaer, NY 1244) and were allowed to acclimatize for one week prior to study enrollment. KO mice were generated as previously described [Grasberger 2012] and bred in-house on a 129S6 background. We provided positive-pressure ventilation at a tidal volume of 10 ml/kg with 2 cmH20 positive end-expiratory pressure (PEEP). Mice were assigned to groups consisting of KO (n = 5) and WT (n = 5) in each group and divided into non-ventilated, positive-pressure ventilation, or LPS IT exposure groups. Positive-pressure ventilation was instituted for 4-h using a FlexiVent (Flexiware 8.1, Scireq, Montreal, QC, Canada). Lipopolysaccharide (Salmonella enterica serotype tryphimurium L6143, Millipore Sigma) was administered via an intratracheal (IT) route at a dose of 0.1 mg/kg. Mice were humanely euthanized at 4-h post-injection consistent with the UC Davis IAUCAC-approved protocol.Results:As previously observed, neutrophilic influx into the airways was significantly impaired in theDuoxa−/−(KO) mice after VILI, but not after LPS exposure. LPS-induced lung injury resulted in upregulation of CD11b+neutrophils and shedding of CD62L and CD162 regardless of DUOX expression, whereas VILI resulted in upregulation of CD49+neutrophils in theDuoxa+/+(WT) mice but not theDuoxa−/−(KO) mice.Conclusion:Our data suggest DUOX is required for CD18-independent mechanisms of neutrophil recruitment in the lung induced by acute lung injury, but not for canonical CD18depedent mechanisms after LPS exposure.
Extracellular vesicle characteristics and microRNA content in cerebral palsy and typically developed individuals at rest and in response to aerobic exercise
Authors: Vechetti Ivan J. , Norrbom Jessica , Alkner Björn , Hjalmarsson Emma , Palmcrantz Alexandra , Pontén Eva , Pingel Jessica , von Walden Ferdinand , Fernandez-Gonzalo Rodrigo 
Published: December 2022

In this study, the properties of circulating extracellular vesicles (EVs) were examined in cerebral palsy (CP) and typically developed (TD) individuals at rest and after aerobic exercise, focusing on the size, concentration, and microRNA cargo of EVs. Nine adult individuals with CP performed a single exercise bout consisting of 45 min of Frame Running, and TD participants completed either 45 min of cycling (n = 10; TD EX) or were enrolled as controls with no exercise (n = 10; TD CON). Blood was drawn before and 30 min after exercise and analyzed for EV concentration, size, and microRNA content. The size of EVs was similar in CP vs. TD, and exercise had no effect. Individuals with CP had an overall lower concentration (∼25%, p &lt; 0.05) of EVs. At baseline, let-7a, let-7b and let-7e were downregulated in individuals with CP compared to TD (p &lt; 0.05), while miR-100 expression was higher, and miR-877 and miR-4433 lower in CP compared to TD after exercise (p &lt; 0.05). Interestingly, miR-486 was upregulated ∼2-fold in the EVs of CP vs. TD both at baseline and after exercise. We then performed an in silico analysis of miR-486 targets and identified the satellite cell stemness factor Pax7 as a target of miR-486. C2C12 myoblasts were cultured with a miR-486 mimetic and RNA-sequencing was performed. Gene enrichment analysis revealed that several genes involved in sarcomerogenesis and extracellular matrix (ECM) were downregulated. Our data suggest that circulating miR-486 transported by EVs is elevated in individuals with CP and that miR-486 alters the transcriptome of myoblasts affecting both ECM- and sarcomerogenesis-related genes, providing a link to the skeletal muscle alterations observed in individuals with CP.
Diminished schwann cell repair responses play a role in delayed diabetes-associated wound healing
Authors: Zhou Shaolong , Wan Lingling , Liu Xu , Hu Delin , Lu Feng , Chen Xihang , Liang Fangguo 
Published: December 2022

Diabetes mellitus is the most common metabolic disease associated with impaired wound healing. Recently, Schwann cells (SCs), the glia of the peripheral nervous system, have been suggested to accelerate normal skin wound healing. However, the roles of SCs in diabetic wound healing are not fully understood. In this study, Full-thickness wounds were made in the dorsal skin of C57/B6 mice and db/db (diabetic) mice. Tissue samples were collected at different time points, and immunohistochemical and immunofluorescence analyses were performed to detect markers of de-differentiated SCs, including myelin basic protein, Sox 10, p75, c-Jun, and Ki67. In addition, in vitro experiments were performed using rat SC (RSC96) and murine fibroblast (L929) cell lines to examine the effects of high glucose conditions (50 mM) on the de-differentiation of SCs and the paracrine effects of SCs on myofibroblast formation. Here, we found that, compared with that in normal mice, wound healing was delayed and SCs failed to rapidly activate a repair program after skin wound injury in diabetic mice. Furthermore, we found that SCs from diabetic mice displayed functional impairments in cell de-differentiation, cell-cycle re-entry, and cell migration. In vitro, hyperglycemia impaired RSC 96 cell de-differentiation, cell-cycle re-entry, and cell migration, as well as their paracrine effects on myofibroblast formation, including the secretion of TGF-β and Timp1. These results suggest that delayed wound healing in diabetes is due in part to a diminished SC repair response and attenuated paracrine effects on myofibroblast formation.
Regulatory mechanism of a heat-activated retrotransposon by DDR complex in Arabidopsis thaliana
Authors: Niu Xiaoying , Chen Lu , Kato Atsushi , Ito Hidetaka 
Published: December 2022

The RNA-directed DNA methylation (RdDM) pathway plays an essential role in the transposon silencing mechanism; the DDR complex, consisting of DRD1, DMS3, and RDM1, is an essential component of the RdDM pathway. ONSEN, identified in Arabidopsis, is a retrotransposon activated by heat stress at 37°C; however, studies on the regulation of ONSEN are limited. In this study, we analyzed the regulation of ONSEN activity by the DDR complex in Arabidopsis. We elucidated that loss of any component of the DDR complex increased ONSEN transcript levels. Transgenerational transposition of ONSEN was observed in the DDR-complex mutants treated with heat stress for 48 h. Furthermore, the DDR complex components DRD1, DMS3, and RDM1 played independent roles in suppressing ONSEN transcription and transposition. Moreover, we found that the duration of heat stress affects ONSEN activity. Therefore, the results of this study provide new insights into the retrotransposon regulatory mechanisms of the DDR complex in the RdDM pathway.
Requests and apologies in two languages among bilingual speakers: A comparison of heritage English speakers and English- and Hebrew-dominant bilinguals
Authors: Bar On Sagit , Meir Natalia 
Published: December 2022

IntroductionLinguistic research over the last two decades has uncovered a significant number of properties that identify heritage language (HL) speakers as a particular phenomenon within bilingualism. However, despite the rapid development of HL research, the sphere of HL speech act pragmatics is still in its infancy.MethodologyThe current study sought to cover part of this gap by investigating both languages of HL adult speakers for whom English is their HL and Hebrew is their dominant societal language (SL;n= 20, hereafter HS) in comparison with two other groups: Hebrew-dominant speakers who were born to Hebrew-speaking families and raised in Israel, and thus English is the language of their schooling (n= 20, hereafter HEB-D), and English-dominant speakers who were born to English-speaking families and immigrated to Israel from an English-speaking country after the age of 18, and thus Hebrew is their L2 (n= 20, hereafter ENG-D). The discourse-pragmatic tasks in English and in Hebrew consisted of the same 36 scenarios eliciting requests and apologies in each language. Each request was followed by an apology that is related.ResultsThe results indicated that Hebrew-dominant speakers and English-dominant speakers, i.e., HEB-D and ENG-D, had different strategies for the realization of requests and apologies which they systematically transferred from their dominant language into their weaker one, confirming the cross-cultural and cross-linguistic differences between request and apology realizations in English and in Hebrew. However, the picture was more complex for the HL speakers in their HL-English and SL-Hebrew as in some cases their strategies paired up with the ENG-D in English, and with the HEB-D in Hebrew, while in other cases they developed a unique and hybrid linguistic style reflecting the strategies of both languages.DiscussionThe investigation of both languages of HL speakers enabled us to compare features of the two linguistic systems and make conclusions about their nature.
A systematic review of the research on telework and organizational economic performance indicators
Authors: Mutiganda Jean Claude , Wiitavaara Birgitta , Heiden Marina , Svensson Sven , Fagerström Arne , Bergström Gunnar , Aboagye Emmanuel 
Published: December 2022

IntroductionA systematic review is conducted in the study to investigate the relationship between telework and organizational economic performance indicators such as self-reported employee performance, organizational performance, actual employee turnover rates, or intentions.MethodsThe databases Scopus, Business Source Premier, and Web of Science were used to conduct a literature search. Original articles published from 2000 and up to May 2021 were selected. Studies were screened for inclusion independently by review pairs and data were extracted. The Mixed Methods Appraisal Tool (MMAT) was used to evaluate the quality of the studies included.ResultsForty-three studies were included with some addressing multiple outcomes. Self-reported performance was higher for teleworking employees compared to those working in the ordinary workplace. The extent of the change in performance was dependent on individual characteristics and the extent of the teleworking practice in the organization. Telework was also associated with increased organizational performance, particularly in homogenous samples with unique work tasks. When telework is voluntary, it appears that both actual employee turnover rates and intentions to leave the organization are lower.DiscussionFurther research with high-quality prospective designs is necessary to properly understand the contribution of telework to organizational economic performance indicators.
Examining the underlying latent structure of the sports emotion questionnaire: Insights from the bifactor multidimensional item response theory
Authors: Hagan John Elvis , Quansah Frank , Ankomah Francis , Agormedah Edmond Kwesi , Srem-Sai Medina , Schack Thomas 
Published: December 2022

BackgroundDespite the widespread use of the sports emotion questionnaire (SEQ) in several studies, it is surprising that only a few have explicitly tested the validity and utility of the instrument in non-western populations. Besides, the issue of dimensionality and the latent structure of the instrument remain inconclusive given that several authors have revealed different factor structures across diverse populations. The central concern is whether the items on the various dimensions, proposed for the original SEQ, offer adequate information to their respective expected subscale or otherwise. This study assessed the underlying latent structure of the SEQ using confirmatory and bifactor multidimensional item response (MIRT) models.MethodsThrough a well-designed validation study 300 athletes from three West African countries, participating in the 2018 West African University Games were surveyed to respond to the SEQ. The data were analyzed using first, a 5-factor confirmatory factor analysis (CFA) via the MIRT model and second, a bifactor MIRT analysis.ResultsThe results revealed that items on the SEQ were fairly good in measuring the construct under the respective domains of the instrument. However, the outcome of the bifactor model showed that the majority of the items on the SEQ explained common variance in relation to the general factor other than the specific domains (5-dimensions).ConclusionFindings of the bifactor model question whether the sub-dimensions of the SEQ are needed since most of the items on the SEQ explained larger variances in the general factor than any of the five domains. It is concluded that instruments like SEQ should be scored for a general factor and not as sub-dimensions. Further investigations are encouraged by scholars within the area to probe the dimensionality of the SEQ.
Measuring depression severity based on facial expression and body movement using deep convolutional neural network
Authors: Liu Dongdong , Liu Bowen , Lin Tao , Liu Guangya , Yang Guoyu , Qi Dezhen , Qiu Ye , Lu Yuer , Yuan Qinmei , Shuai Stella C. , Li Xiang , Liu Ou , Tang Xiangdong , Shuai Jianwei , Cao Yuping , Lin Hai 
Published: December 2022

IntroductionReal-time evaluations of the severity of depressive symptoms are of great significance for the diagnosis and treatment of patients with major depressive disorder (MDD). In clinical practice, the evaluation approaches are mainly based on psychological scales and doctor-patient interviews, which are time-consuming and labor-intensive. Also, the accuracy of results mainly depends on the subjective judgment of the clinician. With the development of artificial intelligence (AI) technology, more and more machine learning methods are used to diagnose depression by appearance characteristics. Most of the previous research focused on the study of single-modal data; however, in recent years, many studies have shown that multi-modal data has better prediction performance than single-modal data. This study aimed to develop a measurement of depression severity from expression and action features and to assess its validity among the patients with MDD.MethodsWe proposed a multi-modal deep convolutional neural network (CNN) to evaluate the severity of depressive symptoms in real-time, which was based on the detection of patients’ facial expression and body movement from videos captured by ordinary cameras. We established behavioral depression degree (BDD) metrics, which combines expression entropy and action entropy to measure the depression severity of MDD patients.ResultsWe found that the information extracted from different modes, when integrated in appropriate proportions, can significantly improve the accuracy of the evaluation, which has not been reported in previous studies. This method presented an over 74% Pearson similarity between BDD and self-rating depression scale (SDS), self-rating anxiety scale (SAS), and Hamilton depression scale (HAMD). In addition, we tracked and evaluated the changes of BDD in patients at different stages of a course of treatment and the results obtained were in agreement with the evaluation from the scales.DiscussionThe BDD can effectively measure the current state of patients’ depression and its changing trend according to the patient’s expression and action features. Our model may provide an automatic auxiliary tool for the diagnosis and treatment of MDD.
Associations of depressive and anxiety symptoms with non-suicidal self-injury and suicidal attempt among Chinese adolescents: The mediation role of sleep quality
Authors: Jiao Ting , Guo Shuangshuang , Zhang Yi , Li Yanqi , Xie Xinyi , Ma Ying , Chen Ruoling , Yu Yizhen , Tang Jie 
Published: December 2022

BackgroundAssociations of depressive and anxiety symptoms with non-suicidal self-injury (NSSI) and suicide attempt (SA) are not well understood. We aimed to examine these associations among Chinese adolescents, and whether any potential association is mediated through sleep quality.MethodsWe conducted a cross-sectional study among 1,771 (994 boys [56.1%] and 777 girls [43.9%], mean [SD] age was 12.9 [0.6] years) adolescents who participated in the baseline survey of the Chinese Adolescent Health Growth Cohort (CAHGC) study. Depressive symptoms, anxiety symptoms, NSSI, SA and sleep quality were measured by validated questionnaire. Logistic regression models were employed to estimate the associations of depression and anxiety with NSSI and SA. Mediation analyses were conducted to explore the mediate effect of sleep quality.ResultsThe 12-month prevalence of NSSI and SA was 17.1 and 8.3%, respectively. Depressive and anxiety symptoms were significantly associated with NSSI (the adjusted odds ratio [aOR] was 1.89 [95% CI 1.34–2.65] for depressive symptoms and 2.84 [95% CI 2.05–3.94] for anxiety symptoms) and SA (the aOR was 3.20 [95% CI 2.03–5.05] for depressive symptoms and 2.98 [95% CI 1.84–4.84] for anxiety symptoms). No significant gender differences were found in the associations. The mediation proportion of sleep quality on the association of depressive and anxiety symptoms with NSSI, as well as depressive and anxiety symptoms with SA were 21.1, 13.9, 13.6, and 14.7, respectively.ConclusionIndependent associations of depressive and anxiety symptoms with NSSI and SA were observed in Chinese adolescents, and there were no significant gender differences in the associations. Moreover, these associations were partially mediated through sleep quality. Targeted interventions for adolescents’ NSSI and SA should focus on those who have depressive and anxiety symptoms, and poor sleep quality.