bluuebunny/crossref_metadata_2025_split huggingface dataset:
Calidad en Doppler materno fetal: Propuesta de una Escala Objetiva Modificada de calidad y auditoría
Authors: Castillo-Urquiaga Walter 
Published: May 2023

La debilidad técnica y en la comprensión hemodinámica genera evaluaciones Doppler de baja calidad con persistencia de desenlaces adversos, altos costos, vigilancia o tratamiento innecesarios y limitado avance del conocimiento fisiopatológico.
La investigación y práctica clínica relacionadas al Doppler materno fetal muestran impacto poco significativo, discrepancias e incluso incongruencias; si bien un porcentaje de esto se genera por la no uniformidad en la terminología diagnóstica, las tablas de referencia y el manejo, otro porcentaje se explica por la baja calidad en la evaluación Doppler.
Las recomendaciones ya establecidas para la calidad Doppler pueden representarse en una herramienta que permita mejorarla y evaluarla objetivamente. Esto podría no solo disminuir los desenlaces adversos sino que tornaría a las investigaciones más uniformes y con evidencias más sólidas.
La calidad en las medidas de los índices Doppler dependen de las técnicas de evaluación y medición, obtención de un espectro óptimo y de su registro en condiciones basales y libre de factores de confusión o intervinientes; la falla en uno o más de ésto genera mediciones falsamente anormales o falsamente normales llevando a manejos innecesarios o pérdida en la oportunidad de evitar desenlaces adversos.
Proponemos una escala modificada con parámetros objetivos de calidad-auditoría para su incorporación a la práctica clínica rutinaria. Esta evalúa 10 criterios: 1) Sitio anatómico, 2) Magnificación, 3) Claridad de imagen, 4) Ángulo de insonación, 5) Velocidad de barrido, 6) PRF y Línea de Base, 7) Volúmen de muestra, 8) Factor de confusión, 9) Filtro adecuado y 10) Confiabilidad.
Hippocampal versus cortical deletion of cholinergic receptor muscarinic 1 in mice differentially affects post-translational modifications and supramolecular assembly of respiratory chain-associated proteins, mitochondrial ultrastructure, and respiration: implications in Alzheimer’s disease
Authors: Sabbir Mohammad Golam , Swanson Mamiko , Speth Robert C. , Albensi Benedict C. 
Published: May 2023

Introduction: In a previous retrospective study using postmortem human brain tissues, we demonstrated that loss of Cholinergic Receptor Muscarinic 1 (CHRM1) in the temporal cortex of a subset of Alzheimer’s patients was associated with poor survival, whereas similar loss in the hippocampus showed no such association. Mitochondrial dysfunction underlies Alzheimer’s pathogenesis. Therefore, to investigate the mechanistic basis of our findings, we evaluated cortical mitochondrial phenotypes in Chrm1 knockout (Chrm1−/−) mice. Cortical Chrm1 loss resulted in reduced respiration, reduced supramolecular assembly of respiratory protein complexes, and caused mitochondrial ultrastructural abnormalities. These mouse-based findings mechanistically linked cortical CHRM1 loss with poor survival of Alzheimer’s patients. However, evaluation of the effect of Chrm1 loss on mouse hippocampal mitochondrial characteristics is necessary to fully understand our retrospective human tissue-based observations. This is the objective of this study.Methods: Enriched hippocampal and cortical mitochondrial fractions (EHMFs/ECMFs, respectively) derived from wild-type and Chrm1−/− mice were used to measure respiration by quantifying real-time oxygen consumption, supramolecular assembly of oxidative phosphorylation (OXPHOS)-associated proteins by blue native polyacrylamide gel electrophoresis, post-translational modifications (PTMs) by isoelectric focusing (IEF), and mitochondrial ultrastructure by electron microscopy.Results: In contrast to our previous observations in Chrm1−/− ECMFs, EHMFs of Chrm1−/− mice significantly increased respiration with a concomitant increase in the supramolecular assembly of OXPHOS-associated proteins, specifically Atp5a and Uqcrc2, with no mitochondrial ultrastructural alterations. IEF of ECMFs and EHMFs from Chrm1−/− mice showed a decrease and an increase, respectively in a negatively charged (pH∼3) fraction of Atp5a relative to the wild-type mice, with a corresponding decrease or increase in the supramolecular assembly of Atp5a and respiration indicating a tissue-specific signaling effect.Discussion: Our findings indicate that loss of Chrm1 in the cortex causes structural, and physiological alterations to mitochondria that compromise neuronal function, whereas Chrm1 loss in the hippocampus may benefit neuronal function by enhancing mitochondrial function. This brain region-specific differential effect of Chrm1 deletion on mitochondrial function supports our human brain region-based findings and Chrm1−/− mouse behavioral phenotypes. Furthermore, our study indicates that Chrm1-mediated brain region-specific differential PTMs of Atp5a may alter complex-V supramolecular assembly which in turn regulates mitochondrial structure-function.
Tight hydrophobic core and flexible helices yield MscL with a high tension gating threshold and a membrane area mechanical strain buffer
Authors: Sharma Arjun , Anishkin Andriy , Sukharev Sergei , Vanegas Juan M. 
Published: May 2023

The mechanosensitive (MS) channel of large conductance, MscL, is the high-tension threshold osmolyte release valve that limits turgor pressure in bacterial cells in the event of drastic hypoosmotic shock. Despite MscL from Mycobacterium tuberculosis (TbMscL) being the first structurally characterized MS channel, its protective mechanism of activation at nearly-lytic tensions has not been fully understood. Here, we describe atomistic simulations of expansion and opening of wild-type (WT) TbMscL in comparison with five of its gain-of-function (GOF) mutants. We show that under far-field membrane tension applied to the edge of the periodic simulation cell, WT TbMscL expands into a funnel-like structure with trans-membrane helices bent by nearly 70°, but does not break its ‘hydrophobic seal’ within extended 20 μs simulations. GOF mutants carrying hydrophilic substitutions in the hydrophobic gate of increasing severity (A20N, V21A, V21N, V21T and V21D) also quickly transition into funnel-shaped conformations but subsequently fully open within 1–8 μs. This shows that solvation of the de-wetted (vapor-locked) constriction is the rate-limiting step in the gating of TbMscL preceded by area-buffering silent expansion. Pre-solvated gates in these GOF mutants reduce this transition barrier according to hydrophilicity and the most severe V21D eliminates it. We predict that the asymmetric shape-change of the periplasmic side of the channel during the silent expansion provides strain-buffering to the outer leaflet thus re-distributing the tension to the inner leaflet, where the gate resides.
Human T-cell leukemia virus type 1 is associated with dysthyroidism in the French Amazon
Authors: Dugardin Julia , Demar Magalie , Hafsi Nezha , Amroun Hakim , Aurelus Jean-Markens , Drak Alsibai Kinan , Ntoutoum André , Santa Florin , Nacher Mathieu , Sabbah Nadia 
Published: May 2023

BackgroundHuman T-cell leukemia virus type 1 (HTLV-1) is a retrovirus known to cause two major diseases: adult T-cell leukemia/lymphoma and a progressive neuromyelopathy—tropical spastic paraparesis. Many viruses may be involved in the pathogenesis of thyroiditis; however, few studies have focused on the role of HTLV-1. We aimed to investigate the association between HTLV-1 and biological thyroid dysfunction.MethodsWe included 357 patients with a positive HTLV-1 serology and thyroid-stimulating hormone assay data between 2012 and 2021 in a hospital in French Guiana; we compared the prevalence of hypothyroidism and hyperthyroidism in this group with that in an HTLV-1-negative control group (722 persons) matched for sex and age.ResultsThe prevalence of hypothyroidism and hyperthyroidism in patients with HTLV-1 infection was significantly higher than that in the control group (11% versus 3.2% and 11.3% versus 2.3%, respectively; p &lt; 0.001).ConclusionOur study shows, for the first time, the association between HTLV-1 and dysthyroidism in a large sample, suggesting that thyroid function exploration should be systematically implemented in this population as this may have an impact on therapeutic management.
Gender-based vulnerabilities for women during natural disasters in Bangladesh
Authors: Fatema Syadani Riyad , East Leah , Islam Shahidul , Usher Kim 
Published: May 2023

IntroductionFactors that negatively impact women's disaster experiences include poor disaster preparedness, lack of access to a safe place, displacement worries, distance from shelters, risk of abuse in shelters, poverty, and low cultural position of women in rural Bangladesh. This study aimed to give voice to women who experienced gender-based vulnerabilities during natural disasters.MethodsThe research was conducted with women in the two disaster-prone areas utilizing a qualitative approach. Women, aged 18 years or above, who were permanent residents of the selected disaster-prone areas and have experienced one or more disasters, were invited to participate in the study. Twenty-four women volunteered to participate. Data were collected through semi-structured in-depth interview recordings containing questions on sociodemographic characteristics and open-ended questions to assess gender-based vulnerabilities during disasters; data were analyzed using thematic analysis techniques.ResultsOne main theme and six sub-themes emerged from the analysis. Women were often in the middle of making life choices between the risk of being abused and death in disasters. Shelters lacked privacy, heightened the risk of abuse, and women faced social stigma if displaced from their homes during a disaster. The women also experienced health risks because of inadequate hygiene and poor living arrangements such as a lack of adequate shelters, toilet facilities, and secure gender-segregated living arrangements if relocation was essential during a disaster.DiscussionFindings from this study provide guidance to policymakers and disaster aid organizations on disaster preparedness and post-disaster facilities to address the socio-cultural needs of rural women in disaster-prone areas of Bangladesh.
3D-bioprinting of patient-derived cardiac tissue models for studying congenital heart disease
Authors: Wolfe Jayne T. , He Wei , Kim Min-Su , Liang Huan-Ling , Shradhanjali Akankshya , Jurkiewicz Hilda , Freudinger Bonnie P. , Greene Andrew S. , LaDisa John F. , Tayebi Lobat , Mitchell Michael E. , Tomita-Mitchell Aoy , Tefft Brandon J. 
Published: May 2023

IntroductionCongenital heart disease is the leading cause of death related to birth defects and affects 1 out of every 100 live births. Induced pluripotent stem cell technology has allowed for patient-derived cardiomyocytes to be studied in vitro. An approach to bioengineer these cells into a physiologically accurate cardiac tissue model is needed in order to study the disease and evaluate potential treatment strategies.MethodsTo accomplish this, we have developed a protocol to 3D-bioprint cardiac tissue constructs comprised of patient-derived cardiomyocytes within a hydrogel bioink based on laminin-521.ResultsCardiomyocytes remained viable and demonstrated appropriate phenotype and function including spontaneous contraction. Contraction remained consistent during 30 days of culture based on displacement measurements. Furthermore, tissue constructs demonstrated progressive maturation based on sarcomere structure and gene expression analysis. Gene expression analysis also revealed enhanced maturation in 3D constructs compared to 2D cell culture.DiscussionThis combination of patient-derived cardiomyocytes and 3D-bioprinting represents a promising platform for studying congenital heart disease and evaluating individualized treatment strategies.
Low extracellular vesicle concentrations predict survival in patients with heart failure
Authors: Schöler David , Loosen Sven H. , Wirtz Theresa H. , Brozat Jonathan F. , dos Santos Ferreira Grani Lauredana A. , Luedde Tom , Heinrichs Lisa , Frank Derk , Koch Alexander , Roderburg Christoph , Spehlmann Martina E. 
Published: May 2023

BackgroundHeart disease is of worldwide importance due to high morbidity and mortality. Extracellular vesicle (EV) concentration and size represent novel diagnostic and prognostic biomarkers, e.g. in patients with liver cancer, but data on their prognostic relevance in heart disease are lacking. Here, we investigated the role of EV concentration, size and zeta potential in patients with heart disease.MethodsVesicle size distribution, concentration and zeta potential were measured by nanoparticle tracking analysis (NTA) in 28 intensive care unit (ICU) and 20 standard care (SC) patients and 20 healthy controls.ResultsPatients with any disease had a lower zeta potential compared to the healthy controls. Vesicle size (X50) was significantly higher in ICU patients (245 nm) with heart disease as compared to those patients with heart disease receiving standard care (195 nm), or healthy controls (215 nm) (p = 0.001). Notably, EV concentration was lower in ICU patients with heart disease (4.68 × 1010 particles/ml) compared to SC patients with heart disease (7,62 × 1010 particles/ml) and healthy controls (1.50 × 1011 particles/ml) (p = 0.002). Extracellular vesicle concentration is prognostic for overall survival in patients with heart disease. Overall survival is significantly reduced when the vesicle concentration is below 5.55 × 1010 particles/ml. Median overall survival was only 140 days in patients with vesicle concentrations below 5.55 × 1010 particles/ml compared to 211 days in patients with vesicle concentrations above 5.55 × 1010 particles/ml (p = 0.032).SummaryConcentration of EVs is a novel prognostic marker in ICU and SC patients with heart disease.
The implication of chromosomal abnormalities in the surgical outcomes of Chinese pediatric patients with congenital heart disease
Authors: Yu Xiafeng , Tao Yu , Liu Xu , Yu Feng , Jiang Chuan , Xiao Yingying , Zhang Haibo , He Yongrui , Ye Lincai , Wang Ying , Zhou Chunxia , Wang Jian , Jiang Zhengwen , Hong Haifa 
Published: May 2023

BackgroundCopy number variations (CNVs) have been shown to be overrepresented in children with congenital heart disease (CHD). Genetic evaluation of CHD is currently underperformed in China. We sought to determine the occurrence of CNVs in CNV regions with disease-causing potential among a large cohort of Chinese pediatric CHD patients and investigate whether these CNVs could be the important critical modifiers of surgical intervention.MethodsCNVs screenings were performed in 1,762 Chinese children who underwent at least one cardiac surgery. CNV status at over 200 CNV locus with disease-causing potential was analyzed with a high-throughput ligation-dependent probe amplification (HLPA) assay.ResultsWe found 378 out of 1,762 samples (21.45%) to have at least one CNV and 2.38% of them were carrying multiple CNVs. The detection rates of ppCNVs (pathogenic and likely pathogenic CNVs) were 9.19% (162/1,762), significantly higher than that of the healthy Han Chinese individuals from The Database of Genomic Variants archive (9.19% vs. 3.63%; P = 0.0012). CHD cases with ppCNVs had a significantly higher proportion of complex surgeries compared to CHD patients with no ppCNVs (62.35% vs. 37.63%, P &lt; 0.001). Duration of cardiopulmonary bypass and aortic cross clamp procedures were significantly longer in CHD cases with ppCNVs (all P &lt; 0.05), while no group differences were identified for complications of surgery and one-month mortality after surgery. The detection rate of ppCNVs in the atrioventricular septal defect (AVSD) subgroup was significantly higher than that in other subgroups (23.10% vs. 9.70%, P = 0.002).ConclusionsCNV burden is an important contributor to Chinese children with CHD. Our study demonstrated the robustness and diagnostic efficiency of HLPA method in the genetic screening of CNVs in CHD patients.
Quantity and location of aortic valve calcification predicts paravalvular leakage after transcatheter aortic valve replacement: a systematic review and meta-analysis
Authors: Shi Jiale , Li Wei , Zhang Tangshan , Han Chengwen , Wang Zhengjun , Pei Xinhao , Li Xuetao , Zhao Zidong , Wang Pengbo , Han Jingying , Chen Shiqiao 
Published: May 2023

IntroductionTranscatheter aortic valve replacement (TAVR) is the first-line treatment for patients with moderate-to-high surgical risk of severe aortic stenosis. Paravalvular leakage (PVL) is a serious complication of TAVR, and aortic valve calcification contributes to the occurrence of PVL. This study aimed to investigate the effect of location and quantity of calcification in the aortic valve complex (AVC) and left ventricular outflow tract (LVOT) on PVL after TAVR.MethodWe performed a systematic review and meta-analysis to evaluate the effect of quantity and location of aortic valve calcification on PVL after TAVR using observational studies from PubMed and EMBASE databases from inception to February 16, 2022.ResultsTwenty-four observational studies with 6,846 patients were included in the analysis. A high quantity of calcium was observed in 29.6% of the patients; they showed a higher risk of significant PVL. There was heterogeneity between studies (I2 = 15%). In the subgroup analysis, PVL after TAVR was associated with the quantity of aortic valve calcification, especially those located in the LVOT, valve leaflets, and the device landing zone. A high quantity of calcium was associated with PVL, regardless of expandable types or MDCT thresholds used. However, for valves with sealing skirt, the amount of calcium has no significant effect on the incidence of PVL.ConclusionOur study elucidated the effect of aortic valve calcification on PVL and showed that the quantity and location of aortic valve calcification can help predict PVL. Furthermore, our results provide a reference for the selection of MDCT thresholds before TAVR. We also showed that balloon-expandable valves may not be effective in patients with high calcification, and valves with sealing skirts instead of those without sealing skirts should be applied more to prevent PVL from happening.Systematic Review Registrationhttps://www.crd.york.ac.uk/PROSPERO/display_record.php?RecordID=354630, identifier: CRD42022354630.
Computational simulation of the ore-forming processes associated with the Sanshandao-Haiyu gold belt, Jiaodong Peninsula, eastern China: implications for the duration of ore formation
Authors: Shan Wenfa , Mao Xiancheng , Liu Zhankun , Deng Hao , Tang Lei , Wang Dutao , Pan Yong , Liu Yao 
Published: May 2023

The Sanshandao-Haiyu gold belt, Jiaodong Peninsula, Eastern China accounts for more than 1,400 t gold resources, which is always recognized as a coupled result of structural deformation, pore-fluid flow, and hydrothermal alteration-mineralization processes. However, the ore-forming processes associated with this gold belt has not been fully understood by the coupled perspective. In this paper, we use computational modeling to study the spatial distribution of dilation (positive volumetric strain) and the mineralization rate, which can predict the mineralization patterns from a chemical perspective, so as to solve the fully coupled problem during ore-forming processes. The model was loaded on the FLAC3D platform to test four scenarios: 1) Syn-compression in the NW-SE direction; 2) syn-compression in the WNW-ESE direction; 3) syn-compression in the NNW-SSE direction, and 4) syn-extension in the NW-SE direction. The simulation results show that only the syn-compressional scenario can produce wide dilation zones with a distribution pattern similar to the known orebodies. The higher dilation zones with the volumetric strain (&gt;0.1%) and the highest pore-fluid flux are closely associated with the fault geometry, particularly for the gentle segments near the steep-flat transition areas. Besides, the dilation zones also occur with very high absolute values of pore-fluid pressure gradient and temperature gradient, indicating significantly perturbed physical-chemical conditions. The receiver operating characteristic (ROC) curves of the mineralization rate show that the area under the curve (AUC = 0.862) of the NW-SE syn-compression model is the highest among these four scenarios, indicating a major paleo compression stress direction of NW-SE during ore-forming period. Furthermore, based on the mineralization rate, we estimated that the mineralization duration of the Sanshandao-Haiyu gold belt would be less than 100,000 years. These simulation results can not only enhance our understanding of the ore-forming processes in the Sanshandao-Haiyu gold belt and Jiaodong Peninsula but also demonstrate that these can be used directly or indirectly for deep mineral exploration.
Association of size for gestational age and dehydroepiandrosterone sulfate with cardiometabolic risk in central precocious puberty girls
Authors: Zhang Guijiao , Yu Huan , Yu Shengxu , Luo Xiaoping , Liang Yan , Hou Ling , Wu Wei 
Published: May 2023

ObjectiveThe aim of this study was to assess whether size for gestational age and dehydroepiandrosterone sulfate (DHEAS) are associated with cardiometabolic risk in central precocious puberty (CPP) girls.MethodsThe retrospective study included 443 patients with newly diagnosed CPP. Subjects were categorized by birth weight for gestational age (appropriate [AGA], small [SGA], and large [LGA] for gestational age) and serum DHEAS concentration (high [≥75th percentile] and normal [&lt;75th percentile] DHEAS). Cardiometabolic parameters were examined. Composite cardiometabolic risk (CMR) score was calculated based on BMI, blood pressure, glucose, insulin, triglyceride, and HDL cholesterol. Non-obesity CMR score was computed, omitting the value from BMI. Logistic regression models, general linear models, and partial correlation analyses were used to evaluate associations. Propensity score matching was performed for sensitivity analyses.ResultsOverall, 309 patients (69.8%) were born AGA, 80 (18.1%) were born SGA, and 54 (12.2%) were born LGA. Compared with AGA counterparts, CPP girls born SGA were more prone to have elevated HbA1c (adjusted OR = 4.54; 95% CI, 1.43–14.42) and low HDL cholesterol (adjusted OR = 2.33; 95% CI, 1.18–4.61). In contrast, being born LGA was not associated with increased risk for any glucose or lipid derangements. Despite the fact that elevated CMR score was more common among individuals born LGA than AGA (adjusted OR = 1.84; 95% CI, 1.07–4.35), no significant difference was found on non-obesity CMR score (adjusted OR = 0.75; 95% CI, 0.30–1.88). When controlling for age, birth weight SDS, and current BMI-SDS, individuals with high DHEAS exhibited higher HDL cholesterol and apolipoprotein A-1 concentrations and lower triglyceride level and non-obesity CMR score. Furthermore, DHEAS correlated positively with HDL cholesterol and apolipoprotein A-1 and negatively with triglyceride, prominently in girls born SGA, after adjustments for the three abovementioned confounders. Sensitivity analyses corroborated the findings.ConclusionAmong CPP girls, those born SGA were more likely to possess cardiometabolic risk factors compared to their AGA peers. The difference we observed in cardiometabolic risk between individuals born LGA and AGA was driven by BMI. High DHEAS was associated with favorable lipid profile in CPP girls, even in subjects born SGA.