bluuebunny/crossref_metadata_2025_split huggingface dataset:
Dasania phycosphaerae sp. nov., isolated from phytoplankton sample from the south coast of the Republic of Korea
Authors: Jiang Yue , Guan Yong , Kang Sungmo , Lee Mi-Kyung , Kim Ki-Hyun , Li Zhun 
Published: April 2023

A novel bacterial strain, designated as GY-18T, was isolated from particulate material sampled from the Korean south coast. The bacterium was Gram-stain-negative, aerobic and rod-shaped. Oxidase and catalase were positive. Optimal growth was observed at 30 °C, pH 7.0, in the presence of 1 % (w/v) NaCl. Phylogenetic analyses based on 16S rRNA gene and whole-genome sequences suggested that strain GY-18T was a member of the genus

Dasania

, but represented a separate lineage. The 16S rRNA gene sequence of strain GY-18T showed high similarities to

Dasania marina

KCTC 12566T (98.4 %),

Halioglobus japonicus

KCTC 23429T (92.2 %),

Marimicrobium arenosum

KCTC 42300T (92.4 %) and

Seongchinamella unica

KCTC 62383T (92.2 %), respectively. The genome length of strain GY-18T was 3 808 450 bp with a DNA G+C content of 47.8 mol %. The major cellular fatty acids of strain GY-18T were summed feature 3 (comprising C16 : 1
ω7c and/or C16 : 1
ω6c), C16 : 0 and C10 : 0 3-OH (>10 %). Diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and phospholipids were shown to be the major polar lipids. The predominant isoprenoid quinone was Q-8. Based on phylogenetic, phenotypic, chemotaxonomic and genomic features, we propose that strain GY-18T represents a novel species in the genus

Dasania

of the family

Spongiibacteraceae

, with the proposed name Dasania phycosphaerae sp. nov. The type strain is GY-18T (=KCTC 92290T=GDMCC 1.3586T).
Phenotyping Refractory Cardiogenic Shock Patients Receiving Venous-arterial Extracorporeal Membrane Oxygenation with Machine Learning Algorithms
Authors: Wang Shuo , Wang Liangshan , Du Zhongtao , Yang Feng , Hao Xing , Wang Xiaomeng , Shao Chengcheng , Li Chenglong , Wang Hong , Hou Xiaotong 
Published: April 2023

AbstractBackgroundRefractory cardiogenic shock (CS) patients receiving venous-arterial extracorporeal membrane oxygenation (VA-ECMO) have a wide range of mortality, machine algorithm methods may explain the potential heterogeneity of these patients.MethodsBetween January 2018 and May 2021, 210 patients with CS who were receiving VA-ECMO support were enrolled and analyzed retrospectively. The k-means consensus agnostic algorithm was used. Patients were divided into three clusters based on covariates, such as platelet count (PLT), aspartic acid transaminase (AST), Interleukin-6 (IL-6), prothrombin time (PT), and serum lactate level 24 hours after ECMO initiation. The clinical and laboratory profiles were analyzed.ResultsAmong 210 CS with CS receiving ECMO, 148 (70.5%) were men, with a median age of 62 years (interquartile range (IQR): 53-67). Overall, 104 (49.5%) patients survived to discharge with 142 (67.6%) survived on ECMO. The patients were phenotyped into three clusters: (1) “platelet preserved (I)” Phenotype (36 [17.1%] patients), characterized by a preserved platelet count; (2) “hyperinflammatory (II)” phenotype (72 [34.3%] patients), characterized by a significant inflammatory response with higher Interleukin-6 (IL-6), and Interleukin-10 (IL-10) levels; and (3) “hepatic-renal (III)” phenotype (102 [48.6%] patients), characterized by unfavorable conditions in creatinine, aspartic acid transaminase, alanine aminotransferase, direct bilirubin, and prothrombin time. The in-hospital mortality rates were 25.0%, 52.8%, and 55.9% for phenotypes I, II, and III, respectively (P = 0.005).ConclusionThe consensus k-means algorithm analysis identified three phenotypes in refractory patients with CS receiving VA-ECMO: “platelet preserved,” “hyperinflammatory,” and “hepatic-renal.” The phenotypes are associated with the clinical profile and mortality, allowing treatment strategies for subsets of patients with CS receiving ECMO to be developed.
Aerosol Jet Printing Enabled Dual-Function Electrochemical and Colorimetric Biosensor for SARS-CoV-2 Detection
Authors: Liu Li , Xu Zhiheng , Molina Vargas Adrian Moises , Dollery Stephen J. , Schrlau Michael G. , Cormier Denis , O’Connell Mitchell R. , Tobin Gregory J. , Du Ke 
Published: April 2023

ABSTRACTAn aerosol jet printing enabled dual-function biosensor for the sensitive detection of pathogens using SARS-CoV-2 RNA as an example has been developed. A CRISPR-Cas13: guide-RNA complex is activated in the presence of a target RNA, leading to the collateral trans-cleavage of ssRNA probes that contain a horseradish peroxidase (HRP) tag. This, in turn, catalyzes the oxidation of 3,3′,5,5′-tetramethylbenzidine (TMB) by HRP, resulting in a color change and electrochemical signal change. The colorimetric and electrochemical sensing protocol does not require complicated target amplification and probe immobilization and exhibits a detection sensitivity in the femtomolar range. Additionally, our biosensor demonstrates a wide dynamic range of 5 orders of magnitude. This low-cost aerosol inkjet printing technique allows for an amplification-free and integrated dual-function biosensor platform, which operates at physiological temperature and is designed for simple, rapid, and accurate point-of-care (POC) diagnostics in either low-resource settings or hospitals.
WITHDRAWN: Epigenetic clocks and risk assessment in adult spinal deformity: A novel association between pace of aging with frailty, disability, and postoperative complications
Authors: Safaee Michael M. , Dwaraka Varun B. , Lee Justin M. , Fury Marissa , Mendez Tavis L , Smith Ryan , Lin Jue , Smith Dana L. , Burke John F. , Scheer Justin K. , Went Hannah , Ames Christopher P. 
Published: April 2023

Withdrawal statementThe authors have withdrawn their manuscript owing to altered the findings and conclusions related to complication data. The changes in results were due to further scrutiny of the datasets, and led to the removal of 3 patients due to incomplete data. This refinement led to updated results which changed the conclusion of the complication data. Therefore, the authors do not wish this work to be cited as reference for the project. If you have any questions, please contact the corresponding author.
Spiritual and religious aspects influence mental health and viral load: A quantitative study among young people living with HIV in Zimbabwe
Authors: Wüthrich-Grossenbacher Ursula , Mutsinze Abigail , Wolf Ursula , Maponga Charles Chiedza , Midzi Nicholas , Mutsaka-Makuvaza Masceline Jenipher , Merten Sonja 
Published: April 2023

ABSTRACTIntroductionThe role of religion and spirituality as social determinants of health have been widely discussed in and outside the World Health Organization. Studies among people living with Human Immunodeficiency Virus (HIV) describe positive and negative influences of religion and spirituality on health outcome. With a HIV prevalence of 14.8% for females and 8.6% for males, and 22’000 Aids related deaths in 2020, HIV infection remains a life-threatening condition in Zimbabwe, especially for young people. The aim of this research was to measure the influence of religion and spirituality on the health outcome of young people living with HIV in Zimbabwe.MethodsA quantitative questionnaire with three different validated measures of religion and spirituality (Belief in Action Scale, Brief Religious Coping Index, Religious and Spiritual Struggles Scale), demographic, cultural, behavioral, and health questions was administered to 804 young Zvandiri program clients in rural, urban, and peri-urban Zimbabwe between July and October 2021. Regression analysis established significant relations between the result of the three different measures and mental health and viral load results.ResultsReligious coping significantly reduced the probability of common mental disorder, while high religious activity increased the risk. The Religious and Spiritual Struggles scale proved to be a reliable indicator of higher viral loads, risk for treatment failure, and the probability of common mental disorder.ConclusionsThe Religious and Spiritual Struggles scale should be used and validated in other sub-Saharan contexts. It could serve as a new diagnostic tool for the early detection and prevention of treatment failure as well as of common mental disorder.
Trends of health and dietary disparities by economic status among elderly individuals in Japan from 2004 to 2014: A repeated cross-sectional survey
Authors: Machida Daisuke 
Published: April 2023

AbstractThis study examines the trends in health and dietary disparities by economic status among elderly individuals in Japan from 2004 to 2014 with subjective measures. The study design utilized a repeated cross-sectional approach, using data from the Survey of Attitudes among the Elderly toward Daily Life in 2004 and 2014. Logistic regression analysis was performed with subjective economic status, survey year, and their interactions as independent variables, and self-rated health, dietary satisfaction, and intake of balanced meals as dependent variables. The results revealed that disparities in self-rated health, dietary satisfaction, and intake of a balanced meal were present due to economic status. Furthermore, the disparities in self-rated health, dietary satisfaction, and balanced meal intake by economic status remained unchanged from 2004 to 2014 (p for interaction ≥ 0.05). The findings were consistent in sensitivity analyses conducted on those aged 75 and older, as well as on long-term care insurance recipients.
No evidence for trans-generational immune priming inDrosophila melanogaster
Authors: Radhika R. , Lazzaro Brian P. 
Published: April 2023

ABSTRACTMost organisms are under constant and repeated exposure to pathogens, leading to perpetual natural selection for more effective ways to fight-off infections. This could include the evolution of memory-based immunity to increase protection from repeatedly-encountered pathogens both within and across generations. There is mixed evidence for intra- and trans-generational priming in non-vertebrates, which lack the antibody-mediated acquired immunity characteristic of vertebrates. In this work, we tested for trans-generational immune priming in adult offspring of the fruit fly,Drosophila melanogaster, after maternal challenge with 10 different bacterial pathogens. We focused on natural opportunistic pathogens ofDrosophilaspanning a range of virulence from 10% to 100% host mortality. We infected mothers via septic injury and tested for enhanced resistance to infection in their adult offspring, measured as the ability to suppress bacterial proliferation and survive infection. We categorized the mothers into four classes for each bacterium tested: those that survived infection, those that succumbed to infection, sterile-injury controls, and uninjured controls. We found no evidence for trans-generational priming by any class of mother in response to any of the bacteria.
Induced pluripotent stem cell model revealed impaired neurovascular interaction in genetic small vessel disease CADASIL
Authors: Zhang Wenjun , Zhao Xiangjun , Qi Xuewei , Kimber Susan J , Hooper Nigel , Wang Tao 
Published: April 2023

AbstractCerebral Autosomal Dominant Arteriopathy with Subcortical Infarcts and Leukoencephalopathy (CADASIL) is the most common genetic small vessel disease caused by variants in theNOTCH3gene. Patients with CADASIL experience recurrent strokes, developing into cognitive defect and vascular dementia. CADASIL is a late-onset vascular condition, but migraine and brain MRI lesions appear in CADASIL patients as early as their teens and twenties, suggesting an abnormal neurovascular interaction at the neurovascular unit (NVU) where microvessels meet the brain parenchyma. To understand the molecular mechanisms of CADASIL, we established induced pluripotent stem cell (iPSC) models from CADASIL patients and differentiated the iPSCs into the major NVU cell types including brain microvascular endothelial-like cells (BMECs), vascular mural cells (MCs), astrocytes and cortical projection neurons. We then built anin vitroNVU model by co-culturing different neurovascular cell types in Transwells and evaluated the blood brain barrier (BBB) function by measuring transendothelial electrical resistance (TEER). Results showed that, while the wild-type MCs, astrocytes and neurons could all independently and significantly enhance TEER values of the iPSC-BMECs, such capability of MCs from iPSCs of CADASIL patients was significantly impeded. Additionally, the barrier function of the BMECs from CADASIL iPSCs was significantly impaired, accompanied with disorganised tight junctions in iPSC-BMECs, which could not be effectively rescued by the wild-type MCs, astrocytes and neurons. Our findings provide new insight into early disease pathologies on the neurovascular interaction and BBB function at the molecular and cellular levels for CADASIL, which helps inform future therapeutic development.
The impact of earnings volatility, environmental uncertainty and COVID-19 pandemic on accounting comparability in an emerging economy
Authors: Arianpoor Arash , Esmailzadeh Asali Farideh 
Published: April 2023

PurposeThe present study investigated the impact of earnings volatility and environmental uncertainty on accounting comparability in an emerging economy and the moderating role of COVID-19 pandemic for the companies listed on Tehran Stock Exchange (TSE).Design/methodology/approachThe data about 181 companies during 2014–2021 were examined. In this study, accounting comparability was predicted for the firms' accounting systems and the coefficient estimates were calculated. The present study used the coefficient of variation of sales to capture sales volatility as the primary environmental uncertainty measure.FindingsThe results showed that both the earnings volatility and environmental uncertainty have a significant negative effect on accounting comparability, and that COVID-19 significantly increases the negative impact of earnings volatility and environmental uncertainty on accounting comparability. The hypothesis testing based on robust, GLS, GMM, GLM, OLS regressions and t+1 test confirmed these results.Originality/valueThe present study aimed to develop knowledge-providing benefits for companies about the accounting comparability and managing more efficient decisions. The present findings help investors to understand and evaluate the performance of firms more accurately especially in earnings volatility and environmental uncertainty conditions and in the wake of a pandemic crisis such as COVID-19.
Leveraging (in)formal early-mover and diligent-follower advantage mechanisms to attain international niche market leadership: insights from Basque “hidden champions”
Authors: Kamp Bart , Ruiz de Apodaca Iñigo 
Published: April 2023

Purpose
This paper aims to study whether international niche market leaders (INMLs) gained their leading position as early mover or diligent follower, and assess whether they leveraged hard or soft forms of technological, supply pre-emption and customer lock-in advantage mechanisms.

Design/methodology/approach
Empirical material stems from qualitative and quantitative data on a sample of 20 niche companies from the Basque Country (Spain) that operate in business to business markets.

Findings
The sample predominantly followed an early entrant strategy and applied soft measures to reach niche market leadership.

Research limitations/implications
Findings imply that early entering fosters conquering leadership in niche markets, that pioneer advantage is easier to sustain in niches than in mainstream markets, and that soft measures are more effective in niche markets than in larger markets. A limitation to our findings is that they follow from explorative research on a sample of firms from a reduced geographic setting.

Practical implications
Hidden champions and INMLs can be important sources of technological progress and economic value for the localities that host them. Therefore, despite their traditional low profile and the fact that they are not always the largest firms around, policymakers may want to pay more attention to this type of companies.

Originality/value
Tot he best of the authors’ knowledge, this is the first paper to research entry timing and its outcome for market leadership with regard to niche players or hidden champions-type of firms. It introduces an original taxonomy to operationalize and distinguish between hard and soft measures to leverage advantage mechanisms related to market entry timing.
Stock market prediction, COVID-19 pandemic and neural networks: an SCG algorithm application
Authors: Goel Himanshu , Som Bhupender Kumar 
Published: April 2023

PurposeThis study aims to predict the Indian stock market (Nifty 50) by employing macroeconomic variables as input variables identified from the literature for two sub periods, i.e. the pre-coronavirus disease 2019 (COVID-19) (June 2011–February 2020) and during the COVID-19 (March 2020–June 2021).Design/methodology/approachSecondary data on macroeconomic variables and Nifty 50 index spanning a period of last ten years starting from 2011 to 2021 have been from various government and regulatory websites. Also, an artificial neural network (ANN) model was trained with the scaled conjugate gradient algorithm for predicting the National Stock exchange's (NSE) flagship index Nifty 50.FindingsThe findings of the study reveal that Scaled Conjugate Gradient (SCG) algorithm achieved 96.99% accuracy in predicting the Indian stock market in the pre-COVID-19 scenario. On the contrary, the proposed ANN model achieved 99.85% accuracy in during the COVID-19 period. The findings of this study have implications for investors, portfolio managers, domestic and foreign institution investors, etc.Originality/valueThe novelty of this study lies in the fact that are hardly any studies that forecasts the Indian stock market using artificial neural networks in the pre and during COVID-19 periods.
How digital transformation helps enterprises achieve high-quality development? Empirical evidence from Chinese listed companies
Authors: Wu Ye , Li Haohui , Luo Ruiyu , Yu Yubing 
Published: April 2023

PurposeThe purpose of this study is to explore how digital transformation helps enterprises achieve high-quality development, including the mediating mechanism of information transparency, innovation capacity and financial stability, the moderating role of financing constraints and government subsidies, and the heterogeneous effects of property rights, size and growth.Design/methodology/approachThis study conducts two-way fixed-effect model using 780 samples of China's Shanghai-Shenzhen A-share listed companies from 2012 to 2019.FindingsThe results show that digital transformation can effectively improve the total factor productivity (TFP) of enterprises through the triple channels of information transparency, innovation capability and financial stability. Meanwhile, financing constraints significantly inhibited the contribution of digital transformation to TFP, while government subsidies significantly increased the contribution of digital transformation to TFP. In addition, state-owned enterprises (SOEs), large enterprises and high-growth enterprises are more able to achieve high-quality development by increasing their digital transformation.Practical implicationsIn the process of implementing digital transformation, companies should actively improve information transparency, financial stability and innovation capabilities, and choose differentiated paths based on intrinsic characteristics such as property rights, scale and growth. At the same time, the government should actively improve not only the digital institutional environment but also the financial policy and credit system.Originality/valueThis study enriches the theoretical research framework of digital transformation and high-quality development by identifying the channel mechanisms and boundary conditions through which digital transformation affects high-quality development and expands the consequences of digital transformation and the antecedents of high-quality development.
Do different types of vocational education and training programmes influence earnings? Recent evidence from India
Authors: Vincent Andrea , Rajasekhar Durgam 
Published: April 2023

PurposeIndian government initiated several skill development policies and different types of vocational education and training (VET). Yet the participation in skill education is low because of poor labour market outcomes. This paper aims to calculate returns to skill education to understand the type of training that will have better labour market outcomes.Design/methodology/approachIn this paper nationally representative data from the periodic labour force survey (PLFS), collected by the national sample survey office for 2017–2018, are used to estimate the returns to formal and non-formal VET obtained (after different levels of general education) with the help of Heckman's two-stage method.FindingsNearly 8% of the working-age population has received some form of VET (mostly non-formal), generating poor returns. For the overall population, formal on-job training (OJT) and full-time VET influence wage positively and significantly. Full-time VET obtained after secondary and below levels of education generates positive returns, whereas part-time VET is profitable only to those without formal education. At the graduate level, technical education obtained along with VET is associated with better wages.Originality/valueIn India where a considerable proportion of the workforce is employed in the informal sector, different types of skill training like full-time, part-time and OJT influence labour market outcomes. This finding has policy implication for countries with large informal sector and calls for further research in such countries.
Actually existing vitality rights: resisting neoliberal affects at a video game design camp
Authors: Robinson Bradley , Wright William Terrell 
Published: April 2023

PurposeThe purpose of this study is to demonstrate the power of affective pedagogies and playful literacies to resist neoliberal framings of video game play and design in educational contexts.Design/methodology/approachFocusing on the Giga-Games Camp, a video game design camp for adolescents, the authors mobilize different methodological impulses across a number of different registers, using interview data to trace institutional arcs, focal frames from a GoPro camera to see vitality in action and descriptions of platform events to follow these lines through the shift to online instruction brought on by the COVID-19 pandemic.FindingsThe authors narrate three transversal movements of the Giga-Games Camp to reveal how play-centered pedagogies can challenge the neoliberal tendency to assimilate young people’s video gaming practices as a vehicle for future-proof science, technology, engineering and mathematics learning.Originality/valueThe authors offer the concept ofactually existing vitality rightsto describe how attending seriously to vitality in learning spaces will often manifest organically in very real strategies to reimagine and restructure preexisting, neoliberally sedimented uses of space, institutional configurations and constellations of sociopolitical power.