bluuebunny/crossref_metadata_2025_split huggingface dataset:
A new genus and new species of eriophyid mites from Papua New Guinea: a potential biological control agent of Falcataria moluccana (Fabaceae)
Authors: Vidović Biljana , Cvrković Tatjana , Orapa Warea 
Published: January 2023

Abstract

A new genus and species of the subfamily Nothopodinae, Colopodacini from Papua New Guinea are described and illustrated.Solenidiversum falcatariaegen. nov. sp. nov. infestsFalcataria moluccana(Miq.) Barneby & Grimes (Fabaceae) and causes erineum on the leaf surfaces. A key to the genera of the Colopodacini tribe is given.
Vibrational Spectrum and Randomness of Water at the Interface of a Protein-DNA Complex
Authors: Mondal Sandip , Ghanta Krishna Prasad , Mondal Souvik , Bandyopadhyay Sanjoy 
Published: January 2023

Abstract
Molecular dynamics simulations have been carried out to explore the effect of complex formation between the N-terminal domain of the \la-repressor protein (in dimeric form) and the corresponding DNA operator on low-frequency intermolecular vibrational modes of water confined at the interface. The calculations demonstrated enhanced back-scattering of interfacial water due to increased caging effects, the effect being greater for water molecules that are associated with direct binding process. Highest degree of caging effect has been identified with the water molecules that are engaged in forming hydrogen-bonded bridges either near directly bound residues or in establishing contacts between the unbound DNA and protein residues. This leads to blue shifts of the O...O...O bending mode of water and the effect is maximum for the bridged water. The analyses further demonstrated that the local randomness of the interfacial water molecules strongly depends on the conformational rigidity of the DNA and the protein components.
A numerical study of entropy generation in dissipative and radiative Casson hybrid ferrofluid past a thin needle
Authors: Ogunseye H. A. , Agbaje T. M. , Salawu S. O. , Fatunmbi E. O. 
Published: January 2023

Abstract
The interest of raising thermal conductivity and optimizing industrial based materials for an enhanced efficiency and productivity has propels various studies on nanomaterials and non-Newtonian fluids. The uses of nanotechnology in technological advancement and industrial promotion could not be overstressed. Thus, the aim of this study is to numerically investigate the entropy formation, heat propagation and conduction of hybridized Casson ferrofluid with dissipation and radiation in a thin needle. The inherent heat transfer capability of magneto-hybridized Fe2O3 and Al2O3 solid nanoparticles in H2O based solvent motivated the study. The streaming nanofluid is controlled by magnetic field, heat generation and continuous stretching velocity. A partial derivative mathematical model is developed to describe the flow dimensions, and via similarity quantities, an invariant transformation of the model is obtained. A numerical and localized spectral linearization scheme is adopted for the thermodynamic and parameters sensitivities analyzes of the thermal fluid. As observed, the heat transfer comparison rate establishes that the thermal propagation of Fe2O3-Al2O3/water is 8.28% higher than Al2O3/water at 0.01% of volume fraction. The entropy formation is raised with rising nanoparticle size, and radiation and dissipation terms boosted temperature distribution.
Testing Monetary Policy Trilemma for Middle Eastern Economies under a Bayesian Panel VAR
Authors: Shaheen Rozina , Almaktoom Abdulaziz T. 
Published: January 2023

Abstract
Middle Eastern economies follow the dollar-pegged exchange rate policy with an open capital account, and this poses a question regarding the autonomy of the monetary policy stance adopted by the regional central banks. In this context, the current research aims to evaluate the ‘monetary policy trilemma’ whilst using the Bayesian panel vector autoregression approach. In considering four variables, namely domestic interbank interest rate, domestic liquidity, oil price and federal funds rate, the present research finds that a positive shock in US federal funds rate increases the domestic interest rates in the Middle Eastern economies. Additionally, this research finds a negative relationship between the oil price shocks and the domestic interest rates, but, conversely, a positive shock in US federal funds rate induces a reduction in the oil price. The current project is unique, as it examines the monetary policy trilemma whilst considering oil price as a control variable in the system under a time-varying Bayesian panel vector autoregression specification. It provides insights that policymakers can use to determine the autonomy of the domestic monetary policy stance, so as to achieve the broader macroeconomic objectives of that policy.
Identification and Analysis of Toxins in Novel Bacillus thuringiensis Strain Bt S3076-1 against Spodoptera frugiperda and Helicoverpa armigera
Authors: Yang Tianbao , Wu Zhongqi , Li Liuping , Jiang Mingguo , Fang Xuanjun , Huang Wenshan , Zhou Yan 
Published: January 2023

Abstract
Despite the successful application of toxins from Bacillus thuringiensis as biological control agents against pests, new toxins having higher toxicity and broad-spectrum activity to insects are identified more and more. To find new toxins, in this study, based on the whole genome sequence of novel Bacillus thuringiensis strain Bt S3076-1, ten predicted toxic genes were identified, including six cry genes, two tpp genes, one cyt gene and one vip gene, among which six were novel toxins. Subsequently, SDS-PAGE analysis showed that the major proteins at spore maturation stage were about 120 kDa, 70 kDa, 67 kDa, 60 kDa and 40 kDa, while approximately 70 kDa and 40 kDa active proteins after trypsin digestion with the LC50 of 149.64 µg/g and 441.47 µg/g against Spodoptera frugiperda and Helicoverpa armigera larvae, respectively. Furthermore, pathological observation results showed the peritrophic membrane of Spodoptera frugiperda and Helicoverpa armigera larvae was degraded. These findings will provide experimental reference for further research on insecticidal activity, toxicity spectrum as well as the synergism of these toxins in Bt S3076-1.
Assessment of humoral and cellular immunity after bivalent BNT162b2 vaccination and potential association with reactogenicity
Authors: Salvagno Gian Luca , Pighi Laura , Henry Brandon M. , Valentini Myriam , Tonin Beatrice , Bragantini Damiano , Gianfilippi Gianluca , De Nitto Simone , Plebani Mario , Lippi Giuseppe 
Published: January 2023

Abstract
Background: This study investigated the feasibility and clinical value of using a novel, automated and high-throughput SARS-CoV-2 Interferon Gamma Release Assay (IGRA), combined with total anti-SARS-CoV-2 antibodies assessment, for evaluating the immune response after bivalent BNT162b2 vaccination.
Methods: A cohort of healthcare workers, who already underwent primary vaccination and boosting with monovalent BNT162b2 vaccine, received a booster dose of the new BNT162b2 bivalent formulation. Blood samples were taken immediately before vaccination (T0) and 1 month afterwards (T1). Humoral and cellular immunity were assayed with Roche Elecsys Anti-SARS-CoV-2 and Roche Elecsys IGRA SARS-CoV-2, respectively.
Results: The study population consisted of 51 subjects (median age: 43 years; 51% females). Total anti-SARS-CoV-2 antibodies and IGRA SARS-CoV-2 values increased at T1 from 9050 to 25000 BAU/mL (p<0.001), and from 0.44 to 0.78 IU/mL (p=0.385), accounting for median increase of 2.0 and 1.6 folds, respectively. Increased T1 values of total anti-SARS-CoV-2 antibodies and IGRA SARS-CoV-2 were recorded in 100% and 68.6% subjects, respectively. In those with baseline values below the median, post-vaccine levels displayed larger increases of 3.3 and 5.1 folds for anti-SARS-CoV-2 total antibodies and IGRA SARS-CoV-2, respectively. The variation of total anti-SARS-CoV-2 antibodies was inversely associated with their T0 values (r= -0.97; p<0.001), whilst that of IGRA SARS-CoV-2 was inversely associated with its T0 value (r= -0.58; p<0.001). No other associations were found with demographical or clinical variables, including side effects.
Conclusion: The bivalent BNT162b2 vaccine booster enhance humoral and cellular immunity against SARS-CoV-2, especially in recipients with a lower baseline biological protection.
Justification of Chemotherapy Duration in Patients with Multiple and Pre-Extensive Drug Resistant Tuberculosis in the Russian Federation
Authors: Gayda A. I. , Abramchenko A. V. , Romanova M. I. , Toichkina T. V. , Burykhin V. S. , Borisov S. E. , Filippov A. V. , Kuznetsova T. A. , Perkhin D. V. , Sveshnikova O. M. , Lekhlyayder M. V. , Panteleev A. M. , Toinova S. V. , Maslennikova T. I. , Galakhova D. O. , Populyashina L. N. , Saenko G. I. , Anisimova E. S. , Svicharskaya A. K. , Sosova N. A. , Lovacheva O. V. , Maryandyshev A. O. , Samoylova A. G. 
Published: January 2023

The objective: to justify the optimal duration of chemotherapy with Lzd and Bdq by evaluating the long-term treatment outcomes in patients with multiple/pre-extensive drug resistant (MDR/preXDR) tuberculosis who interrupted treatment at different time points.Subjects and Methods. 800 patients with MDR/preXDR tuberculosis were enrolled in the study, they were from all regions of the Russian Federation and they started a course of chemotherapy with regimens containing Lzd and Bdq in 2017-2018. All patients who interrupted their chemotherapy (124) were divided at 4 groups. Group 1-43 patients who received less than 90 doses, Group 2-37 patients who received from 91 to 180 doses, Group 3-39 patients who received from 181 to 270 doses, and Group 4-5 patients who received from 271 to 360 doses of anti-tuberculosis drugs.Results. In Group 3, the effectiveness of reaching a favorable outcome during observation for 3-4 years made 53.8% in 21/39, which was comparable to the effectiveness of treatment with a 24-month course of chemotherapy (2017 – 58.0 and 2018 – 52.1%). The treatment duration of patients from Group 3 was additionally analyzed, the arithmetic mean of the number of administered doses makes 262 ± 15, which allowed recommending 9-month courses of chemotherapy.
Myostatin Serum Related with Sarcopenia Among Elderly Population
Authors: Kartika Ronald Winardi 
Published: January 2023

BackgroundÂ Sarcopenia is a decrease in muscle mass accompanied by a decrease in muscle strength and performance. Sarcopenia arises from a disturbance of the complex balance between anabolic and catabolic factors. Myostatin strongly influences the inhibition of muscle growth by increasing muscle catabolism. Increased myostatin will atrophy in skeletal muscle. In the aging process, there will be an increase in the hormone myostatin plays an active role in the process of sarcopenia. The cause of the increase in myostatin hormone is a chronic inflammatory process that occurs in the aging process.Methods The writing of this paper is a literature review of the role of myostatin in sarcopenia that occurs due to the aging process.Results The incidence of sarcopenia in the elderly was 45 people (64.3%). The results of the analysis were a significant difference between myostatin levels in sarcopenia (47.59 ng/mL) and non-sarcopenia (39.7 ng/mL) subjects. The limit of myostatin levels that can cause an increase in muscle catabolic processes is 48.91 ng/mL. The prevalence ratio of the incidence of sarcopenia based on myostatin levels in the elderly was 3.84, while based on the combined risk of age and myostatin levels was 9.75.Conclusion From the literature review, it was found that there was a significant difference in myostatin levels between the elderly with and without sarcopenia. The prevalence of high myostatin levels in the elderly is almost 4 times higher than in young adults. This will lead to the progression of sarcopenia.
Pandora's Box: assessing the current trends and challenges of IWRM in the uMngeni catchment
Authors: Awuah Akosua Kyerewaa , Stuart-Hill Sabine , Trotter-Boardman Dayle 
Published: January 2023

AbstractThe uMngeni catchment in KwaZulu-Natal faces numerous challenges that threaten the availability and quality of water resources. To understand the prevalent issues, the purpose of the study was to assess the institutional aspects that may or may not have facilitated Integrated Water Resource Management (IWRM). Twenty-one semi-structured interviews were conducted, and development planning and environmental management tools were analysed. Water User Associations (WUAs) are statutory bodies and have not been established at the local level. Moreover, an operational Catchment Management Agency (CMA) at the regional level is non-existent. Consequently, the implementation of IWRM has been very limited. The establishment of the uMngeni Ecological Infrastructure Partnership (UEIP) has facilitated the integration of role-players in the absence of an operational CMA. Most of the spatial planning and environmental management tools feature water resource planning except for the integrated waste management plans. As a result, poor solid waste management contributes to the poor water quality in the uMngeni catchment. The challenges remaining are the poor implementation of plans due to a lack of human and financial resources. Therefore, the gap created by a non-existent operational CMA means catchment management activities will continue to negatively affect water resources and the degree to which water resource management is integrated.
GSK3β/ITCH/c-FLIP Axis Counteracts TRAIL-induced Apoptosis in
Human Lung Adenocarcinoma Cells
Authors: Zheng Xiaofen , Huang Donglan , Liu Xiaohui , Liu Qiu-Yu , Gao Xuejuan , Liu Langxia 
Published: January 2023

Aims:
Further investigation on the mechanism of action of tumor necrosis factor-related
apoptosis-inducing ligand (TRAIL) in NSCLC would shed light on the understanding of TRAIL
resistance and provide new clues for the counter-strategy.

Background:
Cellular FLICE-inhibitory protein (c-FLIP) is a critical inhibitor of TRAIL-induced
apoptosis. Our previous study suggested that glycogen synthase kinase 3β (GSK3β) positively
regulated c-FLIP expression in human lung adenocarcinoma cells. Meanwhile, other studies reported
that c-FLIP was degraded by HECT-type E3 ligase ITCH (Itchy E3 Ubiquitin Protein Ligase) via the
proteasome pathway.

Objective:
We will explore whether ITCH is involved in the expression regulation of c-FLIP
positively controlled by GSK3β during the treatment of TRAIL.

Methods:
Human lung adenocarcinoma cells were used to stably overexpress and knockdown GSK3β.
Quantitative real-time PCR (qRT-PCR) assay was used to test the expressional level of mRNA of
genes. Western blot analysis was employed to detect the expression of proteins at the protein level.
siRNA of ITCH was used to knock down its expression. TRAIL treatment was used to cause
apoptosis.

Results:
In the present study, we have confirmed the degradation of c-FLIP by ITCH protein and the
downregulation of ITCH expression by GSK3β in lung adenocarcinoma cells. Moreover, ITCH
silencing reversed the downregulation of c-FLIP protein caused by GSK3β-knockdown in the cells.
Accordingly, TRAIL-induced apoptosis facilitated by GSK3β knockdown was blocked by the
combined interference of ITCH.

Conclusion:
These results suggested that GSK3β/ITCH axis regulated the stability of c-FLIP and
influenced TRAIL-induced apoptosis. Taken together, our study revealed a GSK3β/ITCH/c-FLIP
axis, which counteracts TRAIL-induced apoptosis in human lung adenocarcinoma cells.
Benefits of GLP-1 Mimetics on Epicardial Adiposity
Authors: Yaribeygi Habib , Maleki Mina , Nasimi Fatemeh , Jamialahmadi Tannaz , Stanford Fatima C. , Sahebkar Amirhossein 
Published: January 2023

Abstract:
The epicardial adipose tissue, which is referred to as fats surrounding the myocardium,
is an active organ able to induce cardiovascular problems in pathophysiologic
conditions through several pathways, such as inflammation, fibrosis, fat infiltration, and
electrophysiologic problems. So, control of its volume and thickness, especially in patients
with diabetes, is highly important. Incretin-based pharmacologic agents are newly
developed antidiabetics that could provide further cardiovascular benefits through control
and modulating epicardial adiposity. They can reduce cardiovascular risks by rapidly
reducing epicardial adipose tissues, improving cardiac efficiency. We are at the first
steps of a long way, but current evidence demonstrates the sum of possible mechanisms.
In this study, we evaluate epicardial adiposity in physiologic and pathologic states and
the impact of incretin-based drugs.
Changes of Colon in Rats with Different Ages in Response to
Lipopolysaccharide
Authors: Li Yanli , Guo Yuhui , Aoqi Liu , Ma Chengquan , Xiong Zhengguo , Yuan Ding , Zhang Changcheng , Zhang Jihong , Dun Yaoyan 
Published: January 2023

Background:
Lipopolysaccharide (LPS) is an endotoxin that causes inflammation,
and the content of LPS increases gradually during the process of aging. Whether the
response of the colon to LPS stimulation will increase with age is yet unknown.

Objective:
The study investigated the effects of LPS stimulation on the colon of adult
and aging rats.

Methods:
43 healthy male SD rats were divided into 4 different groups: adult group and
LPS-stimulated adult group at the age of 4 months, and aging group and LPS-stimulated
aging group at the age of 22 months. Rats were stimulated by intraperitoneal injection of
LPS (1mg/kg) for 24 h. The morphological changes of the colon were observed, and intestinal
inflammatory response, tight junction proteins, apoptosis, and proliferation in intestinal
epithelial cells were detected.

Results:
A series of morphology changes occurred in the colon of adult rats after LPS stimulation,
the higher inflammatory response (TLR4, NF-κB, and IL-1β), changes in the
protein levels of tight junctions (ZO-1, Claudin1, and Claudin2), and increased apoptosis
(Bax, Bcl2) and proliferation (PCNA) of intestinal epithelial cells. The above changes
were also found in aging rats. LPS stimulation further promotes the above changes to
some extent in the colon of aging rats.

Conclusion:
A series of colon changes in rats was significantly damaged during LPS stimulation
and aging, and these changes were further aggravated to some extent in LPS-stimulated
aging rats.
Identification of Differentially Expressed Proteins in Rats with Early Subacute
Spinal Cord Injury using an iTRAQ-based Quantitative Analysis
Authors: Lou Yongfu , Lv Yigang , Li Zhen , Kang Yi , Hou Mengfan , Fu Zheng , Lu Lu , Liu Lu , Cai Zhiwei , Qi Zhangyang , Jian Huan , Shen Wenyuan , Li Xueying , Zhou Hengxing , Feng Shiqing 
Published: January 2023

Background:
Injuries to the central nervous system (CNS), such as spinal cord injury
(SCI), may devastate families and society. Subacute SCI may majorly impact secondary damage
during the transitional period between the acute and subacute phases. A range of CNS illnesses has
been linked to changes in the level of protein expression. However, the importance of proteins during
the early subacute stage of SCI remains unknown. The role of proteins in the early subacute
phase of SCI has not been established yet.

Methods:
SCI-induced damage in rats was studied using isobaric tagging for relative and absolute
protein quantification (iTRAQ) to identify proteins that differed in expression 3 days after the injury,
as well as proteins that did not alter in expression. Differentially expressed proteins (DEPs)
were analyzed employing Gene Ontology (GO) enrichment and Kyoto Encyclopedia of Genes and
Genomes (KEGG) analysis to discover the biological processes, cell components, and molecular
functions of the proteins. We also performed Gene Set Enrichment Analysis (GSEA) software BP
pathway and KEGG analysis on all proteins to further identify their functions. In addition, the first
15 key nodes of a protein-protein interaction (PPI) system were found.

Results:
During the early subacute stage of SCI, we identified 176 DEPs in total between the control and damage groups, with 114 (64.77%) being up-regulated and 62 (35.23%) being down-regulated. As a result of this study, we discovered the most important cellular components and molecular activities, as well as biological processes and pathways, in the early subacute phase of SCI. The top 15 high-degree core nodes were Alb, Plg, F2, Serpina1, Fgg, Apoa1, Vim, Hpx, Apoe, Agt, Ambp, Pcna, Gc, F12， and Gfap.

Conclusion:
Our study could provide new views on regulating the pathogenesis of proteins in the
early subacute phase after SCI, which provides a theoretical basis for exploring more effective
therapeutic targets for SCI in the future.
Influence of Seashell on the Physical, Mechanical and Thermal Properties of Brick Mortar Mixtures
Authors: Essalem Mohamed Lemine Mohamed , Cherradi Toufik , Amziane Sofiane , Teguedy Mohamed Cheikh 
Published: January 2023

Background:
In this work, the effect of crushed cockle shell (CCS) and mixed seashell (MS) as shell sand in cement hydraulic lime brick mortar was investigated. The seashells aggregates came from the shell middens of Mauritania.

Objective:
This study aims to valorize seashell waste in mortar brick mixes, to design a light insulating brick

Methods:
The seashells were heat treated at 105°C for 6h, then crushed and sieved into shell sand. Then, they were studied by X-ray diffraction (XRD), thermogravimetric analysis (TGA), and specific tests for mortar aggregates. Afterward, several mortar bricks were fabricated by replacing the natural sand with shell sand. The physical, mechanical, and thermal properties of the produced bricks were compared with two types of natural mortar bricks. In total Six types of masonry bricks were produced. The properties analyzed were the compressive strength, flexural strength, water absorption, bulk density and bulk porosity, thermal conductivity, and thermal resistance of the bricks.

Results:
It was observed that the use of seashell waste increased the porosity and water absorption of the bricks and decreased the bulk density and mechanical strength.

Conclusion:
It is concluded that the seashell shape promotes the porosity of the mortar bricks making them more insulating and lighter. The characterization of crushed cockle shells (CCS) and mixed seashell (MS) bricks helps to clarify the field and limits of application of these bricks.