bluuebunny/crossref_metadata_2025_split huggingface dataset:
The expression characteristics of transmembrane protein genes in pancreatic ductal adenocarcinoma through comprehensive analysis of bulk and single-cell RNA sequence
Authors: Ye Chen , Ren Siqian , Sadula Abuduhaibaier , Guo Xin , Yuan Meng , Meng Meng , Li Gang , Zhang Xiaowei , Yuan Chunhui 
Published: May 2023

BackgroundTransmembrane (TMEM) protein genes are a class of proteins that spans membranes and function to many physiological processes. However, there is very little known about TMEM gene expression, especially in cancer tissue. Using single-cell and bulk RNA sequence may facilitate the understanding of this poorly characterized protein genes in PDAC.MethodsWe selected the TMEM family genes through the Human Protein Atlas and characterized their expression by single-cell and bulk transcriptomic datasets. Identification of the key TMEM genes was performed through three machine learning algorithms: LASSO, SVM-RFE and RF-SRC. Then, we established TMEM gene riskscore and estimate its implication in predicting survival and response to systematic therapy. Additionally, we explored the difference and impact of TMEM gene expression in PDAC through immunohistochemistry and cell line research.Results5 key TMEM genes (ANO1, TMEM59, TMEM204, TMEM205, TMEM92) were selected based on the single-cell analysis and machine learning survival outcomes. Patients stratified into the high and low-risk groups based on TMEM riskscore, were observed with distinct overall survival in internal and external datasets. Moreover, through bulk RNA-sequence and immunohistochemical staining we verified the protein expression of TMEM genes in PDAC and revealed TMEM92 as an essential regulator of pancreatic cancer cell proliferation, migration, and invasion.ConclusionOur study on TMEM gene expression and behavior in PDAC has revealed unique characteristics, offering potential for precise therapeutic approaches. Insights into molecular mechanisms expand understanding of PDAC complexity and TMEM gene roles. Such knowledge may inform targeted therapy development, benefiting patients.
Association between ultrasound BI-RADS signs and molecular typing of invasive breast cancer
Authors: Pan Qiao-Hong , Zhang Zheng-Pin , Yan Liu-Yi , Jia Ning-Rui , Ren Xin-Yu , Wu Bei-Ke , Hao Yu-Bing , Li Zhi-Fang 
Published: May 2023

ObjectiveTo explore the correlation between ultrasound images and molecular typing of invasive breast cancer, so as to analyze the predictive value of preoperative ultrasound for invasive breast cancer.Methods302 invasive breast cancer patients were enrolled in Heping Hospital affiliated to Changzhi Medical College in Shanxi, China during 2020 to 2022. All patients accepted ultrasonic and pathological examination, and all pathological tissues received molecular typing with immunohistochemical (IHC) staining. The relevance between different molecular typings and ultrasonic image, pathology were evaluated.ResultsUnivariate analysis: among the four molecular typings, there were significant differences in tumor size, shape, margin, lymph node and histological grade (P&lt;0.05). 1. Size: Luminal A tumor was smaller (69.4%), Basal -like type tumors are mostly larger (60.9%); 2. Shape: Basal-like type is more likely to show regular shape (45.7%); 3. Margin: Luminal A and Luminal B mostly are not circumscribed (79.6%, 74.8%), Basal -like type shows circumscribed(52.2%); 4. Lymph nodes: Luminal A type tends to be normal (87.8%), Luminal B type,Her-2+ type and Basal-like type tend to be abnormal (35.6%,36.4% and 39.1%). There was no significant difference in mass orientation, echo pattern, rear echo and calcification (P&gt;0.05). Multivariate analysis: Basal-like breast cancer mostly showed regular shape, circumscribed margin and abnormal lymph nodes (P&lt;0.05).ConclusionThere are differences in the ultrasound manifestations of different molecular typings of breast cancer, and ultrasound features can be used as a potential imaging index to provide important information for the precise diagnosis and treatment of breast cancer.
Corylin accelerated wound healing through SIRT1 and PI3K/AKT signaling: a candidate remedy for chronic non-healing wounds
Authors: Xiu Yanghui , Su Yu , Gao Lihua , Yuan Hui , Xu Sennan , Liu Ying , Qiu Yan , Liu Zhen , Li Yuhang 
Published: May 2023

Introduction: Chronic non-healing wound is a considerable clinical challenge and research into the discovery of novel pro-healing agents is underway as existing therapeutic approaches cannot sufficiently meet current needs.Method: We studied the effects of corylin in cell line fibroblasts and macrophages by Western blots, PCR, Flow cytometry assay, Immunofluorescence.Results: We showed that corylin, a main flavonoid extracted from Psoralea corylifolia L, reduced inflammatory responses, promoted collagen deposition, and accelerated the healing of full-thickness skin wounds in mice. Exploration of the underlying mechanisms showed that corylin activated the PI3K/AKT signaling, leading to fibroblasts’ migration, proliferation, and scratch healing. Corylin also activated sirtuin 1 (SIRT1) signaling, enhanced the deacetylation and cytoplasmic translocation of NF-κB p65, and therefore reduced lipopolysaccharide (LPS)-induced inflammatory responses in macrophages. Furthermore, inhibition of PI3K/AKT and sirtuin 1 pathway with LY294002 and EX527 prevent the therapeutic potency of corylin against chronic wounds.Conclusion: In summary, our results suggested that corylin may be a candidate for the development of novel pro-healing agents.
Latin American perceptions of fear and exaggeration transmitted by the media with regard to COVID-19: frequency and association with severe mental pathologies
Authors: Mejia Christian R. , Aveiro-Róbalo Telmo Raul , Garlisi Torales Luciana Daniela , Castro Hidalgo Verónica Alejandra Alejandra , Valeriano Jhino , Ibarra-Montenegro David Alfonso , Conde-Escobar Aram , Sánchez-Soto Fernanda , Canaviri-Murillo Yuliana , Oliva-Ponce María , Serna-Alarcón Victor , Vilela-Estrada Martín A. , Arias-Chávez Dennis 
Published: May 2023

IntroductionThe COVID-19 pandemic contributed to the spread of abundant misinformation by the media, which caused fear and concern.ObjectiveTo determine the association between the pathologies of the mental sphere and the perceptions of fear and exaggeration transmitted by the media with respect to COVID-19 in Latin America.MethodologyThe present study has an analytical cross-sectional design that is based on a validated survey to measure fear and exaggeration transmitted by the media and other sources (Cronbach's α: 0.90). We surveyed more than 6,000 people, originally from 12 Latin American countries, who associated this perceived exaggeration with stress, depression, and anxiety (measured through DASS-21, Cronbach's α: 0.96).ResultsSocial networks (40%) or television (34%) were perceived as the sources that exaggerate the magnitude of the events. In addition, television (35%) and social networks (28%) were perceived as the sources that generate much fear. On the contrary, physicians and health personnel are the sources that exaggerated less (10%) or provoked less fear (14%). Through a multivariate model, we found a higher level of global perception that was associated with whether the participant was older (p = 0.002), had severe or more serious anxiety (p = 0.033), or had stress (p = 0,037). However, in comparison with Peru (the most affected country), there was a lower level of perception in Chile (p &lt; 0.001), Paraguay (p = 0.001), Mexico (p &lt; 0.001), Ecuador (p = 0.001), and Costa Rica (p = 0.042). All of them were adjusted for gender and for those having severe or major depression.ConclusionThere exists an association between some mental pathologies and the perception that the media does not provide moderate information.
Urban cultural heritage is mentally restorative: an experimental study based on multiple psychophysiological measures
Authors: Wang ShuSheng , Xu Yuqian , Yang Xinyi , Zhang Yuan , Yan Pei , Jiang Yuan , Wang Kai 
Published: May 2023

IntroductionUrban cultural heritage sites bear the cultural functions of a city, hold spiritual and cultural value, can recall emotional memories, and serve the cultural leisure activities of the residents. Urban cultural heritage sites can help citizens perceive a sense of belonging and a feeling of relaxation, but whether and to what extent cultural heritage sites affect mental health remains unknown.MethodsBased on attention restoration theory, multiple research methods are adopted in this study to examine the impact of cultural heritage on human restorative mechanisms. Five representative cultural heritage sites from the cultural heritage-rich city of Xi'an are selected as the research object. In addition, a questionnaire survey and physiological experiments are conducted. Perceived restorative scale, skin conductance response, heart rate variability, and eye movement data while viewing photographs of the cases are collected from the participants.ResultsResults show that cultural heritage sites have psychophysiological restorative effects, which are especially significant in the fascination dimension. Moreover, historical buildings can promote the restorative effects of cultural heritage sites.DiscussionThis finding may lead to new conservation and innovation planning strategies considering the mental health effects of cultural heritage.
Motor cortical functional connectivity changes due to short-term immobilization of upper limb: an fNIRS case report
Authors: Karumattu Manattu Arun , Borrell Jordan A. , Copeland Christopher , Fraser Kaitlin , Zuniga Jorge M. 
Published: May 2023

IntroductionA short-term immobilization of one hand affects musculoskeletal functions, and the associated brain network adapts to the alterations happening to the body due to injuries. It was hypothesized that the injury-associated temporary disuse of the upper limb would alter the functional interactions of the motor cortical processes and will produce long-term changes throughout the immobilization and post-immobilization period.MethodsThe case participant (male, 12 years old, right arm immobilized for clavicle fracture) was scanned using optical imaging technology of fNIRS over immobilization and post-immobilization. Pre-task data was collected for 3 min for RSFC analysis, processed, and analyzed using the Brain AnalyzIR toolbox. Connectivity was measured using Pearson correlation coefficients (R) from NIRS Toolbox's connectivity module.ResultsThe non-affected hand task presented an increased ipsilateral response during the immobilization period, which then decreased over the follow-up visits. The right-hand task showed a bilateral activation pattern following immobilization, but the contralateral activation pattern was restored during the 1-year follow-up visit. Significant differences in the average connection strength over the study period were observed. The average Connection strength decreased from the third week of immobilization and continued to be lower than the baseline value. Global network efficiency decreased in weeks two and three, while the network settled into a higher efficient state during the follow-up periods after post-immobilization.DiscussionShort-term immobilization of the upper limb is shown to have cortical changes in terms of activations of brain regions as well as connectivity. The short-term dis-use of the upper limb has shifted the unilateral activation pattern to the bilateral coactivation of the motor cortex from both hemispheres. Resting-state data reveals a disruption in the motor cortical network during the immobilization phase, and the network is reorganized into an efficient network over 1 year after the injury. Understanding such cortical reorganization could be informative for studying the recovery from neurological disorders affecting motor control in the future.
Bilateral anterior congenital radial head dislocation in adults: a case report and literature review
Authors: Gao Jiahao , Tang Jinshuo , Li Menglong , Li Haitao , Peng Yachen , Wang Chenyu , Liu Tong , Zuo Jianlin 
Published: May 2023

ObjectiveCongenital dislocation of the radial head (CRHD) is a rare condition, with bilateral anterior cases being even less common worldwide. Only a few cases had residual pain after adulthood, even when left untreated. Herein, we describe an adult case of bilateral anterior CRHD with significant pain and snapping during motion. The aim of this study was to report the physical and radiological findings, treatment methods, and short-term outcomes of our case and to review adult CRHD cases in the literature.PatientA 21-year-old male patient presented to our hospital with chief complaints of snapping and exacerbated pain during motion in his left elbow.Diagnoses and interventionsDetailed medical history and physical examination results were recorded. Radiographic examinations were performed on the bilateral elbow, and the diagnosis of bilateral anterior congenital radial head dislocation was confirmed. To relieve the pain and snapping in the left elbow, we performed open reduction and fixation of the radial head with annular ligament reconstruction and ulnar osteotomy. Postoperatively, the elbow rested at 90° flexion with a cast for 16 weeks, and the K-wire was removed on the 10th week; afterward, active functional exercises were performed.OutcomesThe patient was followed-up for 1 year. The pain in his left elbow was relieved with a reduction in the visual analog scale score from 7 to 3. The range of motion of the left elbow was changed from 0° to 135° (preoperative) to −5° to 120° (postoperative) (extension–flexion) without any snapping. However, restrictions in external rotation have not yet been fully resolved. Further physical rehabilitation is required.ConclusionWhen managing patients with congenital radial head dislocation, the contralateral elbow should be evaluated to identify potential bilateral cases. Surgical options should be discussed with adult patients only for the strong need for functional improvement, although the outcomes may not be fully satisfactory.
Significance of predicted future liver remnant volume on liver failure risk after major hepatectomy: a case matched comparative study
Authors: Piccus R. , Joshi K. , Hodson J. , Bartlett D. , Chatzizacharias N. , Dasari B. , Isaac J. , Marudanayagam R. , Mirza D. F. , Roberts J. K. , Sutcliffe R. P. 
Published: May 2023

IntroductionFuture liver remnant volume (FLRV), a risk factor for liver failure (PHLF) after major hepatectomy (MH), is not routinely measured. This study aimed to evaluate the association between FLRV and PHLF.Patients and methodsAll patients undergoing MH (4 + segments) between 2011 and 2018 were identified from a prospectively maintained single-centre database. Perioperative data were collected for patients with PHLF, who were matched (1:2) with non-PHLF controls. FLRV and FLRV% (i.e., % of total liver volume) were calculated retrospectively from preoperative CT scans using Synapse-3D software, and compared between the PHLF and matched control groups.ResultsOf 711 patients undergoing MH, PHLF occurred in 27 (3.8%), of whom 24 had preoperative CT scans available. These patients were matched to 48 non-PHLF controls, 98% of whom were classified as being at high risk of PHLF on preoperative risk scoring. FLRV% was significantly lower in the PHLF group, compared to matched controls (median: 28.7 vs. 35.2%, p = 0.010), with FLRV% &lt; 30% in 58% and 29% of patients, respectively. Assessment of the ability of FLRV% to differentiate between PHLF and matched controls returned an area under the ROC curve of 0.69, and an optimal cut-off value of FLRV% &lt; 31.5%, which yielded 79% sensitivity and 67% specificity.ConclusionsFLRV% is significantly predictive of PHLF after MH, with over half of patients with PHLF having FLRV% &lt; 30%. In light of this, we propose that all patients should undergo risk stratification prior to MH, with the high risk patients additionally being assessed with CT volumetry.
UJI EFEKTIFITAS PEMBERIAN KOMPOSISI MEDIA TANAM DAN APLIKASI POC NASA TERHADAP PERTUMBUHAN DAN HASIL TANAMAN SELEDRI (APIUM GRAVEOLENS L.)
Authors: Jihat Almu 
Published: May 2023

Penelitian dilaksanakan di Kelurahan Pekkabata, Kecamatan Polewali, kabupaten polewali mandar, provinsi sulawesi barat yang berlangsung dari bulan Agustus sampai Desember 2021. Bertujuan untuk mengetahui efektifitas pemberian komposisi media tanam dan aplikasi POC Nasa terhadap pertumbuhan dan produksi tanaman seledri (Apium Graveolens L). Penelitian ini menggunakan Rancangan Acak Kelompok dengan pola Faktorial, yang terdiri dari 2 faktor yaitu Faktor pertama pemberian POC Nasa yang terdiri dari 3 taraf yaitu : tanpa pemeberian POC Nasa (P0), 3 ml/ liter air (P1) dan 5 ml/ liter air (P2). Faktor kedua Pemberian komposisi media tanam yang terdiri dari 3 taraf yaitu : ( M1) tanah + arang sekam + pupuk kandang kambing (1:2:1), (M2) tanah + arang sekam + pupuk kandang kambing (2:1:1) dan (M3) tanah + arang sekam + pupuk kandang kambing (1:1:2). Hasil statistik menunjukkan bahwa interaksi pemberian komposisi media tanam dan aplikasi POC Nasa (LxP) memberikan pengaruh pada parameter tinggi tanaman. Pemberian komposisi media tanam tanah + arang sekam + pupuk kandang kambing (1:1:2) memberikan pengaruh terbaik pada parameter jumlah daun, jumlah anakan, berat tanaman dan panjang akar. Kata Kunci : pertumbuhan seledri, pemberian POC Nasa, komposisi media tanam.
Optimization of integration of jaw bone tissue by cellular structures made of titanium impregnated with ascorbic acid
Authors: Vasilyuk V.P. , Chetvertnykh V.A. 
Published: May 2023

The article presents a study on improving the efficiency of osseointegration processes in the plastic of jaw defects by using ascorbic acid in an experiment.
Structured implants with cells of 250 microns, obtained by growing on the Realizer SLM 50 installation, were implanted in guinea pigs in the lower jaw area (observation period of 1 month). For this purpose, 2 groups were formed: the main one — 5 animals and the comparison group — 3 animals. Ascorbic acid was a stimulant for the growth of connective and bone tissue. A method of introducing it into the implant cells has been developed. Studies were carried out on Altami MET-5D, a universal metallographic microscope used to work in reflected light at a magnification of 10 times.
As a result of the study, the IV degree of fixation prevailed in the main group (33.3±0.2%), the IV degree of fixation was also revealed in the comparison group (25±0.2%). Using an inductor (Vit. C) coarse-fibrous bone tissue was formed in implants with cells measuring 850 microns (term of 1 month), and in the absence of a modulator, connective tissue and cartilaginous tissue, penetrated by a network of small vessels, prevailed at the same time.
It is concluded that implants with a face-centered cubic crystal lattice structure have sufficiently high integration properties. At the same time, the use of vitamin C as a stimulant significantly increases the growth and integration of both connective and various types of bone tissue.
Adolescence et radicalisation : une nouvelle conduite à risque
Authors: Chantepy-Touil Céline 
Published: May 2023

Qui sont ces jeunes qui s’engagent dans un processus de radicalisation islamiste ? Comment comprendre leur fascination pour une croyance religieuse aussi radicale et violente dans ses préceptes ? Quel sens, trouver à ces parcours d’adolescents qui « bifurquent » de leur histoire familiale pour s’engager dans un processus jusqu’ à renier leur propre filiation ? Telles sont les premières questions que Céline Chantepy-Touil, sociologue, a pu se poser au cours des 6 années où elle a dirigé un dispositif dédié à la prise en charge des personnes signalées radicalisées dans le Rhône. Au contact de ces profils jeunes, elle livre ici sa compréhension de ce phénomène, que l’on pourrait associer par certains égards à une nouvelle conduite à risques.
Generation of nonlinear polarization to emit terahertz waves in above gap states in GaSb/AlGaSb multiple quantum wells
Authors: Kojima Osamu , Osaki Shinnosuke , Kita Takashi , Akahane Kouichi 
Published: May 2023

Abstract
In this study, we report the generation of frequency-tunable even-order nonlinear polarization in the above gap states in strained multiple quantum wells. Using the strain field, the even-order polarization to emit terahertz waves is generated in a zincblende crystal. Our results can make the material and laser choice ranges wide because the exciton resonant excitation is unnecessary.
The Karakterisasi dan Skrinning Fitokimia Simplisia Sabut Kelapa Muda
Authors: Salim Reny , Taslim Tuty , Dewi Irene Puspa 
Published: May 2023

Latar Belakang: Sabut kelapa muda merupakan salah satu simplisia yang telah diuji mempunyai manfaat sebagai antibakteri dan antiseptik. Sabut kelapa muda sebagai salah satu simplisia herbal bagi pengobatan belum pernah dikarakterisasi dan diidentifikasi kandungan metabolit sekunder yang dimiliki. Penelitian ini bertujuan untuk mengetahui nilai parameter standar mutu simplisia serbuk sabut kelapa muda dan kandungan metabolit sekunder yang dimilikinya.
Metode: Karakterisasi serbuk simplisia sabut kelapa muda berupa uji organoleptis, kadar air, kadar sari larut air, dan kadar sari larut etanol. Metode penentuan kadar air menggunakan metode destilasi azeotropic kontiniu. Metode uji organoleptis dan kadar sari larut air dan larut etanol sesuai dengan Farmakope Herbal Indonesia. Skrinning fitokimia serbuk simplisia sabut kelapa muda menggunakan uji Mayer, uji Dragendroff, uji Bouchardat, Shinoda test, Liebermann Buchard test, saponin test, polifenol test.
Hasil: Serbuk simpilisia mempunyai warna dark salmon pink, berbau khas lemah, kadar air, kadar sari larut air dan sari larut etanol secara berurutan sebesar (6,94; 16,85; 18,35)%. Jenis senyawa metabolit sekunder yang terdapat pada serbuk simplisia sabut kelapa muda adalah alkaloid, flavonoid, tanin, saponin, dan triterpenoid.
Kesimpulan:Nilai parameter standar mutu serbuk simplisia sabut kelapa muda adalah warna dark salmon pink, berbau khas lemah, kadar air, kadar sari larut air dan sari larut etanol secara berurutan sebesar (6,94; 16,85; 18,35)% serta jenis senyawa metabolit sekunder yang terdapat pada serbuk simplisia sabut kelapa muda adalah alkaloid, flavonoid, tanin, saponin, dan triterpenoid.
Kata kunci : karakterisasi simplisia, skrinning fitokimia simplisia, sabut kelapa muda
PENGEMBANGAN MODEL PARTISIPATIF MASYARAKAT DALAM MENCIPTAKAN LINGKUNGAN YANG SEHAT, BERKUALITAS DAN PRODUKTIF (SEMADU) BAGI MASYARAKAT DI MASA PANDEMI COVID-19 DI DESA SUKADAMAI CIKUPA
Authors: Yuliati  , Abdurrasyid  , Rian Adi Pamungkas  
Published: May 2023

Pendahuluan: Keadaan sehat adalah kehendak semua pihak, tidak hanya di dominasi oleh perorangan, akan tetapi juga harus dimiliki oleh kelompok dan bahkan oleh masyarakat. Desayang bersih menjadi sebuah cerminan bagi setiap individu dalam menjaga kesehatan jasmani dalam kehidupan sehari-hari. Sebagaimana diketahui bahwa kehidupan manusia sendiri tidak bisa dipisahkan baik lingkungan alam maupun lingkungan sosial. Maka seharusnya segala aspek yang ada dalam masyarakat dapat menjaga kebersihan lingkungan.
Metode: Metode yang digunakan yaitu survey, sosialisasi kepada masyarakat, dan memberikan edukasi pada waega desa
Hasil: Masyarakat yang peduli akan desanya untuk menjadi seterkait Sehat, Produktif dan Berkualitas bagi masyarakat di masa pandemi COVID-19 Desa Sukadamai, melalui Senam kaki diabetes pada Lansia, didapatkan data 40 % masyarakat LANSIA mengikuti kegiatan edukasi dan demonstrasi dalam melakukan Senam kaki diabetes dengan baik dan antusias.
Kesimpulan: Masyarakat memberikan respon yang baik dan berkomitmen ikut serta dalam membentuk desa Sehat dan Mandiri