bluuebunny/crossref_metadata_2025_split huggingface dataset:
Prediction of Dexter’s Soil Quality Index Using Soil Physical, Chemical, and Geophysical Properties
Authors: Mesri Samira , Ghorbani-Dashtaki Shoja , Shirani Hossein , Kamkar-Rouhani Abolghasem , Motaghian Hamidreza 
Published: May 2023

Abstract
Dexter’s soil physical quality index (the S index), which is defined as the slope of the soil-water characteristic curve at the inflection point, can properly describe the soil physical quality. This study was conducted to derive a model to predict the S index using soil physical, chemical, and geophysical properties. For this, 72 soil samples were collected, and some of the physical, geophysical, and chemical properties of the soil samples were measured. The soil water content was measured using sand-box and pressure plate apparatus. RETC software was also used to determine the parameters of the van Genuchten equation. These parameters were employed to find the slope of the moisture curve at the inflection point as an indicator of soil physical quality. The decision tree analysis was applied to derive the proper model for predicting the S index using different combinations of chemical, physical and geophysical soil properties in 4 scenarios and the most important predictor(s) were determined in each scenario. The effective factors on the S index in the first and third scenarios included the moisture and the mean weight diameter of aggregates; while in the second and fourth scenarios in which some of chemical properties were replaced by geophysical and structural properties, the dielectric constant and mean weight diameter of aggregate were the most influential factors on the S index. The coefficient of determination of 0.86 was obtained as a result of correlation between the measured and predicted data in the first and third scenarios, while in the second and fourth scenarios, it was obtained as 0.87. Considering the values of %RMSE, the modeling in all four scenarios can be regarded as successful. However, the % RMSE value in the second and fourth scenarios was less than that in the first and third scenarios.
Effective methods for immobilization of non-adherent Pv11 cells while maintaining their desiccation tolerance
Authors: Fuse Hiroto , Kikawada Takahiro , Cornette Richard 
Published: May 2023

Abstract
Pv11 is the only animal cell line that can be preserved in the dry state at room temperature. Pv11 was derived from embryos of the sleeping chironomid Polypedilum vanderplanki, which displays an extreme form of desiccation tolerance known as anhydrobiosis. Pre-treatment with a high concentration of trehalose for 48 h allows Pv11 cells to enter anhydrobiosis. In the dry state, Pv11 cells preserve transgenic luciferase while retaining its activity; thus, these cells could be utilized as a vessel for dry-preserving valuable biological materials without loss of activity. However, Pv11 cells grow in suspension, which limits their applicability; for instance, they cannot be integrated into microfluidic devices or used in devices such as sensor chips. Therefore, in this paper, we sought to develop an effective immobilization system for Pv11 cells that, crucially, allows them to maintain their anhydrobiotic potential even when immobilized. First, we examined the effectiveness of various immobilization systems commonly used in standard cell cultures and found that Pv11 cells exhibited a very high adhesion rates with both biocompatible anchor for membrane (BAM) and Cell-Tak coatings. We also found that Pv11 cells immobilized well to uncoated glass if handled in serum-free medium. Next, we investigated whether immobilized Pv11 cells could retain their anhydrobiotic ability. While trehalose treatment of Pv11 cells prior to immobilization allowed them to retain a high level of both desiccation tolerance and proliferative potential after rehydration, trehalose treatment of Pv11 cells after immobilization resulted in a significant decrease in desiccation tolerance. Thus, it is important to induce anhydrobiosis before immobilization. In summary, we report the successful development of a protocol for the dry preservation of immobilized Pv11 cells.
On the design and implementation of a mixed-platform collaborative virtual RAS planner
Authors: Palma Belén , Portalés Cristina , Coma Immaculada , Gimeno Jesús , Casas Sergio 
Published: May 2023

Abstract
Robotic Assisted Surgery (RAS) represents an important step forward in the field of minimally invasive surgery. However, the learning curve of RAS is steep, and a systematic planning of surgical robot setups should be performed to leverage the features of RAS. For this reason, in this paper we show the design and implementation of a mixed-platform collaborative application, creating an interactive virtual shared environment that simulates RAS during the surgery planning phase. The proposed application allows one or more experts to plan together the different phases of an RAS-based surgical procedure, while integrating different levels of immersion to enhance computer-assisted training. We have tested our application with a total of four domain experts. Our results show that experts found the system excellent in terms of usability and useful to prepare and discuss surgical planning with RAS.
Integrated Analysis and Validation Reveal CYTH4 as a Potential Prognostic Biomarker in Acute Myeloid Leukemia
Authors: Wang Hong , Xiao Yishu , Zhou Wei , Li Yonghui 
Published: May 2023

Abstract
Background
Acute myeloid leukemia (AML) is a clonal hematological malignancy with high mortality rates, and the clinical and genomic heterogeneity of AML has complicated therapy development. Identifying novel markers is urgently in need for AML. Cytohesins are a subfamily of guanine nucleotide exchange factors (GEFs) activating ADP-ribosylation factor (ARF) family GTPases. While previous studies have reported the important roles of cytohesins in various cancers, their function in AML remains unclear. Therefore, we performed this study to explore the prognostic impact of cytohesin-4 (CYTH4) and investigate the underlying molecular functions.
Methods
We obtained RNA sequencing data and AML clinical data from The Cancer Genome Atlas (TCGA) and Gene Expression Omnibus (GEO) datasets to investigate gene expression and survival. Using R software, we identified differentially expressed genes between the high-CYTH4 group and the low-CYTH4 group. We conducted functional enrichment analysis by performing GO, KEGG, and GSEA analyses. CIBERSORTx tool was used to explore the proportions of different immune cell types. We also evaluated the molecular function of CYTH4 by examining cell growth, cell cycle, apoptosis, and colony-forming ability using CYTH4-knockdown AML cell lines.
Results
CYTH4 was significantly overexpressed in AML when compared with other cancers and normal tissues. High CYTH4 expression was associated with old age (p = 0.014), complex karyotype (p = 0.048), and higher risk status (p = 0.001). Patients with high CYTH4 expression had poor overall survival (OS) (high vs. low, HR = 1.58, 95%CI 1.04–2.45, p = 0.032) and event-free survival (EFS) (high vs. low, HR = 1.84, 95%CI 1.13–2.94, p = 0.013), and these patients could benefit from transplantation (transplantation vs. chemotherapy, HR = 0.35, 95%CI 0.20–0.60, p = 0.0001). Multivariate analysis showed high CYTH4 expression was independently associated with inferior OS (HR = 1.01, 95%CI 1.00-1.03, p = 0.017) and EFS (HR = 1.02, 95%CI 1.00-1.03, p = 0.034). Functional analysis showed that CYTH4 was involved in immunoregulation. In vitro validation showed knockdown of CYTH4 adversely affected cell growth and induced cell apoptosis.
Conclusions
CYTH4 is highly expressed in AML and can potentially function as a prognostic biomarker.
A Secure Optical Body Area Network Based on Free Space Optics and Time-Delayed 2D-Spectral/Spatial Optical CDMA
Authors: Kanwal Firdos , Armghan Ammar , Imtiaz Waqas A. , Haq Anwar Ul , mirza jawad 
Published: May 2023

Abstract
Free space optics (FSO) based optical body area networks (OBANs) are getting massive attention as an opportunity to address the limitations of radio frequency (RF) based counterparts. This boom in research interest is primarily due to multitude of benefits including, high capacity, immunity to electromagnetic interference (EMI), rapid installation, cost efficiency, license-free use of spectrum etc. The secure transmission of private health data of patients in OBANs over an insecure FSO channel is a challenging task. Therefore, this paper proposes a low cost, flexible, and secure OBAN based on FSO technology and time-delayed two dimensional (2D) spectral/spatial optical code-division multiple access (OCDMA) system. The proposed architecture consists of eight sensors attached to the bodies of patients operating at the rate of 50 kbps. Data from each sensor is encoded using a 2D spectral/spatial double weight-zero cross correlation (DW-ZCC) code. The 2D encoded optical signals are then time delayed to eliminate the multiple parallel FSO channels between the transmitter and medical center. The combined optical signal is transmitted via 1 km of FSO channel towards remote medical center. Received signal is decoded and data of each sensor is recovered after photo-detection at medical center for further analysis. The FSO channel between transmitter and medical center is modeled using Log-normal channel model. The overall performance of the sensors is analyzed using Bit-error rate (BER) plots for distinctive climate conditions. Secure and error free transmission of sensors data is successfully achieved for different weather conditions between the patients and remote medical center while achieving low receiver sensitivities.
Use and efficacy of dry-mouth interventions in Sjögren’s Disease patients and possible association with perceived oral dryness and patients’ discomfort
Authors: Assy Zainab , Santen J. S. , Brand H. S. , Bikker F. J. 
Published: May 2023

Abstract
Objectives Sjögren’s Disease (SjD) patients use various interventions to relief their oral dryness. However, the use and efficacy of these interventions have only partially been evaluated. The present study aims to investigate whether there is an association between the perceived oral dryness and discomfort of SJDpatients and their use of specific interventions.Materials and methods A cross-sectional study was performed among SjD patients, who completed several questionnaires to assess the severity of their oral dryness and an inventory of dry-mouth interventions. The perceived efficacy of each intervention was reported on a 5-point Likert-scale.Results The questionnaires were returned by 92 SjD patients. For relief of oral dryness, they mostly used “eating fruit’’, “drinking tea’’, “moistening the lips’’, “drinking water’’ and “drinking small volumes’’ (> 50%). Three interventions had a frequency of use ranging from 2–6 times/day, whereas, “drinking water’’ and “drinking small volumes’’ showed higher frequencies (> 14). The highest overall efficacy (≥ 3.5) was reported for “chewing gum’’ and “using a mouth gel’’. Furthermore, various dry-mouth interventions showed significant associations with oral dryness scores and/or patients’ discomfort. For example, “drinking small volumes” and “using XyliMelts” were associated with the Bother Index score.Conclusion Great variation was found in the use of dry-mouth interventions by the participants and the severity of the oral dryness and/or patients’ discomfort seemed to affect their choice of intervention. Notably, the mostly used interventions did not show the highest reported efficacy.Clinical relevance: These findings might help SjD patients and clinicians in their choice for effective dry-mouth interventions.
Trace determination of Cr (VI) and Cr in tomato samples: risk assessment in a mining area in Iran
Authors: Dizaji Rana , Abbasi Khadijah , Abdi Khosrou M , kamali Koorosh 
Published: May 2023

Abstract
To assess food safety, the determination of the highly toxic Chromium (VI) concentration in vegetables is important. Accurate and efficient determination of Cr(VI) in agricultural products, due to complex matrices, still remains a challenge. In this study, we aimed to determine the Cr(VI) concentration in tomatoes grown in farmlands, which is potentially contaminated by Pb and Zn mines and factories. We used an alkaline extraction at 80°C, for 5min containing 50 mM EDTA and dispersive liquid liquid miroextraction (DLLME) by spectrophotometric assay. Method validation, including spiking recoveries, showed satisfying results for Cr(VI) determination. Tomatoes were collected from two case studies: A) farmland, where the average concentrations of total Cr in soils was 73,775.51 µg kg− 1 and B) farmland with an average Chromium 24083.68 µg kg− 1. The average concentrations of Cr(VI) and total Cr in tomatoes from the contaminated case study A were 341.037 ± 55.2037 µg kg− 1 and 2539.731 ± 268.3714, respectively, while the lowest average Cr(VI) value was found to be 98.93917 ± 51.13807 and total Cr was 1243.09 ± 562.4392 µg kg− 1, attributed to case study B. In site(A), The total Cr in soil correlated with the Cr in tomatoes (r = 0.548), while a relatively low correlation was found for Cr (VI) in tomatoes (r = 0.309). The evaluation of the potential human health risks associated with tomatoes indicated that inhabitant children are at considerable non-carcinogenic and carcinogenic risks.
Determination of electrical facies using MRGC and ANN clustering methods
Authors: Eftekhari Seyedeh Hajar , Memariani Mahmoud , Maleki Zahra , AleAli Seyed Mohsen , Kianoush Pooria 
Published: May 2023

Abstract
The present research was performed on Iran's most crucial reservoir formation (Asmari Formation) in the Mansouri oil field in southwest Iran. This Formation is generally composed of carbonate lithologic, but it has sandstone layers in some zones. It is also the youngest reservoir rock in Iran. In the first step, the lithology of the Asmari Formation was determined by cross-lithology diagrams, and a combination of lime, dolomite, anhydrite, and sandstone with shaley layers was estimated. Also, in the sequence of this geological/reservoir zoning formation, five zones were identified with different geological/reservoir conditions, and the average of each of these petrophysical parameters was calculated in each zone, with the best reservoir quality in zones 3 and 5 with sandstone/shale lithology. Also, this study determines facies clustering methods MRGC and ANN and reading of gamma, neutron, density, acoustic and resistivity diagrams, facies having common geological/reservoir conditions in different subdivisions. For this purpose, the combined log readings of DT, RHOB, GR, Sw, NPHI, and PHIE were used. After applying the software (Geolog) method, an optimal model with 8 clusters (facies) was obtained. The reservoir features and plots in the software for better separation of rock species studied. Finally, five facies with different lithological compositions and reservoir conditions were identified in this study. The information obtained from determining electrical facies by clustering method determines the reservoir zone from non-reservoir and qualitatively (good, medium, and poor) of Mansouri oilfield were achieved.
Impacts of landscape pattern evolution on typical Ecosystem Services in Ganjiang River Basin, China
Authors: Wu Shiwen , Song Xu , Huang Yun , Wu Hanqing , Liu Zhong , Zhu Jinqi , Wan Wei , Zheng Bofu 
Published: May 2023

Abstract
Understanding the response mechanism of ecosystem services (ES) to landscape patterns is of great significance for regional landscape planning and sustainable development. In this study, the landscape index and InVEST model were used to quantitatively analyze the spatio-temporal evolution of landscape patterns and ES in the Ganjiang River Basin of China from 1990 to 2020. Furthermore, the bivariate Moran's I method and spatial error model (SEM) were used to test the spatial correlation between landscape index and ES. The results showed that (1) cropland decreased and construction land increased, and the overall landscape tended to be fragmented, the patch shape tended to be complicated, and landscape diversity increased from 1990 to 2020. Water conservation (WC) and soil conservation (SC) capacity increased by 10.56 mm and 16.24 t hm–2 a–1, respectively, whereas carbon storage (CS) decreased by 1.22 t hm–2 a–1. (2) The responses of different typical ES to landscape patterns were different in landscape index and response degree. Typical ES negatively responded to Shannon’s diversity index and patch density. WC was sensitive to the Splitting Index, whereas SC and CS were more responsive to the average patch area. (3) The overall purpose of territorial spatial planning within a basin should be to reduce the fragmentation and heterogeneity of the landscape. According to four local aggregation patterns of landscape index and ES, corresponding measures can be taken according to local conditions in different regions. The results can provide a quantitative basis for landscape management and ecological construction in the Ganjiang River basin and scientific guidance for the Yangtze River conservation strategy.
Complement might be the Link between Diabetic Kidney Disease and Immunology: Identification of the Shared Gene Signatures and Molecular Mechanism in Diabetic Kidney Disease and Immune-Related Kidney Diseases
Authors: Dong Zhaocheng , Cao Zijing , Geng Yunling , Zhang Yifei , Wu Yun , Wang Ying , Zhang Chao , Liu Baoli , Liu Yu Ning , Liu Wei Jing 
Published: May 2023

AbstractBackground Diabetic kidney disease (DKD) is a common microvascular complication of diabetes mellitus (DM), which is the main cause of end-stage renal disease and renal replacement therapy. In recent years, more and more studies have described the immune-related mechanisms of kidney injury such as inflammation. But anti-inflammatory therapies are difficult to gain effect. Methods To explore immune-related mechanisms of DKD, we compared it with immune-related kidney diseases using bioinformatics analysis. We searched for DKD and membranous nephropathy (MN), IgA nephropathy (IgAN), lupus nephritis (LN) and anti-neutrophil cytoplasmic autoantibody (ANCA)-associated vasculitis (AAV) in Gene Expression Omnibus (GEO) database. We downloaded RNA sequences and patient information for these five kidney diseases. We used Metascape software to analyze the enrichment of differentially expressed genes (DEGs) and shared genes in these diseases. By drawing the protein-protein interaction (PPI) network map, we screened out the hub genes, and used receiver operating characteristics (ROC) curve to perform diagnostic tests. Results We found that DKD shares 66 pathways with MN, 9 pathways with IgAN, 85 pathways with LN, and 54 pathways with AAV. Among them, the number of up-regulated pathways shared in the glomeruli was the largest. These pathways were mainly immune related pathways such as inflammation. We further analyzed the shared genes between DKD and LN or AAV, and found that the hub genes were C1QA, C1QB, C1R and C1S. These genes are associated with complement activation. Moreover, compared with healthy controls, their abnormal expression levels have diagnostic significance for DKD. At last, we used a network meta-analysis to confirm that current anti-inflammatory therapy is difficult to effectively treat DKD. Conclusions This study suggests that although there may be an immunological cause of renal injury in DKD, complement activation plays the key role in the occurrence and development of DKD. This would provide potential targets for novel therapies of DKD.
Proximity effect between Cu2(OH)3Cl and copper metal
Authors: Pimentel Dyvison Pedreira 
Published: May 2023

Abstract
The change in the spin state of a normal metal in contact with a highly frustrated antiferromagnetic insulator is shown experimentally. Dicopper chloride trihydroxide [Cu2(OH)3Cl] was used as the antiferromagnetic insulator and Copper (Cu) as the normal metal. By the application of a voltage, a conductive path of copper immersed in an antiferromagnetic insulator was created. Cu2(OH)3Cl is a highly insulating antiferromagnet with magnetic transitions at TN1 ~ 18 K and TN2 ~ 6.4 K. Thus, the electric current must flow in the copper path. One can clearly observe a change in resistance as a function of copper temperature that is related to a change in the ordering of the copper spins that was induced by the antiferromagnetic transitions of Cu2(OH)3Cl due to the proximity effect of this layer.
Dapagliflozin Improves Podocytes Injury in Diabetic Nephropathy via Regulating Cholesterol Balance Through KLF5 Targeting the ABCA1 Signalling Pathway
Authors: Wang Qinglian , Zhang Xinyu , Sun Jingshu , Wang Simeng , Chen Dandan , Shu Jianqiang , Chong Nannan , Xu Ying 
Published: May 2023

Abstract
Diabetic nephropathy (DN), one of the more prevalent microvascular complications in patients diagnosed with diabetes mellitus, is attributed as the main cause of end-stage renal disease (ESRD). Lipotoxicity in podocytes caused by hyperglycemia has already been recognised as a significant pathology change, resulting in the deterioration of the glomerular filtration barrier. Research has demonstrated how dapagliflozin, a kind of SGLT2i, exhibits a multifaceted and powerful protective effect in DN, entirely independent of the hypoglycemic effect, with the specific mechanism verified. In this present study, we find that dapagliflozin has the potential to alleviate apoptosis and restore cytoskeleton triggered by high glucose (HG). We also discover that dapagliflozin could mitigate podocyte cholesterol accumulation by restoring the expression of ABCA, which is the key pathway for cholesterol outflows. This research also mechanistically demonstrates that the protective effect of dapagliflozin can be mediated by KLF-5, which is the upstream transcription factor of ABCA1. Results of in vivo experiments also show that dapagliflozin has the potential to alleviate apoptosis and cholesterol accumulation induced by diabetes mellitus condition. The expression of ABCA1 and KLF-5 is also restored by dapagliflozin in vivo. Taken together, our data suggest that dapagliflozin offers significant potential in alleviating podocyte injury and cholesterol accumulation triggered by high glucose. In terms of the mechanism, we herein reveal that dapagliflozin could accelerate cholesterol efflux by restoring the expression of ABCA1, which is directly regulated by KLF-5.
Isospectral drum simulations using FreeFem++
Authors: Costa Masterson M. , de Carvalho Pitágoras P. 
Published: May 2023

Abstract

This research presents a program implemented in the software FreeFem++ to calculate approximate numerical solutions by the finite element method in twoisospectral regions. The original discovery of these domains was made by Gordon, Webb and Wolpert in 1992. The main objective of this work is to examinethe relative differences in corresponding eigenvalues. This paper is a pioneer in demonstrating numerical simulations performed on the well-known problem “Can you hear the shape of a drum?” produced by Mark Kac.
Mathematics Subject Classification (2000) 35J86 · 58J53 · 65N30 · 65K05