bluuebunny/crossref_metadata_2025_split huggingface dataset:
Dynamics of psychophysiological functions of young representatives of precision-target sports at different stages of the training process
Authors: Kozina Zh.L. , Seredenko K. , Parhomenko O. 
Published: March 2023

The purpose of the work is to provide a description of the structure of the psychophysiological functions of young hockey players and a comparative characterization of the development of neurodynamic indicators in hockey players and representatives of cyclical sports.
Material and methods. Psychophysiological testing was carried out using the computer program "Psychodiagnostics", as well as the program developed under the leadership of V.S. Ashanina The research was conducted twice: at the end of the pre-competitive period and at the end of the competitive period.
The results. changes in the structure of the relationship of psychophysiological indicators among themselves according to the data of correlation and factor analysis were found. Both in the first and in the second testing, the investigated indicators comprised 4 factors. But the indicators included in each factor and the percentage values ​​of each factor were slightly different for the first and second testing. In the second testing, i.e. at the end of the competitive period, indicators of simple reactions to sound came to the fore in the structure of the relationship of psychophysiological indicators, while in the pre-competitive period simple reactions to light came to the fore.
Conclusions. The role of complex reactions in the structure of the relationship of psychological indicators increases during the transition from the pre-competition to the end of the competition period. This indicates an increase in the role of mental processes with an increase in the skill of the players.
Binge drinking and violence in the transition to adulthood
Authors: Savage Joanne , Rossler Michael 
Published: March 2023

AbstractWe analyzed data from the National Longitudinal Study of Adolescent Health (Add Health) to test the hypothesis that binge drinking, rather than frequency of any drinking, would predict violent behavior in the transition from adolescence to adulthood (TAA). In conservative models, accounting for a host of factors relevant to the TAA, we find that binge drinking, but not frequency of drinking, is associated with violent behavior. The models included a control for nonviolent offending, conforming to studies of the “differential etiology of violence” thesis. In addition, we tested whether this association fell away among participants over the age of 21 and found that underage status did not mediate the association between binge drinking and violent behavior.
A Dual‐Drug Nanohybrid System Incorporating Nimodipine and Brain‐Derived Neurotrophic Factor Promotes Retinal Ganglion Cells Survival
Authors: Shen Yuening , Zhang Zhutian , Wang Guanhong , Huang Chang , Quan Yadan , Zhang Shenghai , Wu Jihong , Sun Jianguo , Sun Xinghuai 
Published: March 2023

AbstractGlaucoma, a leading cause of irreversible visual impairments and even blindness, is characterized by elevated intraocular pressure and progressive loss of structure and function of neuronal systems. To effectively protect neuronal systems from damage caused by elevated intraocular pressure, a novel dual‐drug nanohybrid has been developed by incorporating brain‐derived neurotrophic factor (BDNF)‐loaded mesoporous silica nanoparticles (BDNF@MSN) into the thermogel matrix with nimodipine (NMD) (BDNF@MSN‐NMD@Thermogel). The carrier materials (i.e., silica and thermogel) in this nanohybrid do not show any cytotoxicity to human lens epithelial cells and rat retinal precursor cells. This nanohybrid regulates the in vitro release of BDNF and NMD in a sustainable way for weeks. In optic nerve crush rodent models, a single intravitreal injection of BDNF@MSN‐NMD@Thermogel inhibits retinal neuroinflammation, significantly promotes retinal ganglion cells survival and restores visual function for nearly three months. This nanohybrid is thus a promising alternative for effective neuroprotection treatment for neuroinflammation‐related neurodegenerative diseases.
Click chemistry in the design of AIEgens for biosensing and bioimaging
Authors: Giel Marie‐Claire , Hong Yuning 
Published: March 2023

AbstractThe development of rapid, selective, and sensitive fluorescent sensors is essential for visualizing and quantifying biological molecules and processes in vitro, ex vitro, and in vivo, which is important for not only fundamental biological studies but the accurate diagnosis of diseases. The emerging field of activity‐based sensing (ABS), a sensing method that utilizes molecular reactivity for analyte detection possesses many advantages including high specificity, sensitivity and accuracy. The aggregation caused quenching phenomenon which occurs in most conventional fluorophores results in reduced labeling efficiency of the target analytes and low photobleaching resistance, therefore limiting the applications of the ABS strategy. In contrast, aggregation induced emission (AIE) active luminogens (AIEgens) provide exceptional molecular frameworks for ABS. Of the many reaction classes utilized in the AIEgen ABS approach, click chemistry has become increasing popular. In this review, the sensing concepts of the ABS approach with AIEgens and the principles of click chemistry are discussed, followed by a systematic summary of the application of specific click chemistry reactions in AIEgen ABS protocols for the detection of an array of target analytes. Furthermore, the utility of click chemistry in the construction of AIEgens for bioimaging will also be showcased throughout the review.
A gated strategy stabilizes room‐temperature phosphorescence
Authors: Gu Kaizhi , Meng Zhengong , Liu Xing Wang , Wu Yue , Qi Xin , Ren Yiran , Yu Zhen‐Qiang , Tang Ben Zhong 
Published: March 2023

AbstractRoom‐temperature phosphorescence (RTP) of purely organic materials is easily quenched with unexpected purposes because the excited triplet state is extremely susceptible to external stimuli. How to stabilize the RTP property of purely organic luminogens is still challenging and considered as the bottleneck in the further advancement of the bottom‐up approach. Here, we describe a gated strategy that can effectively harness RTP by employing complexation/dissociation with proton. Due to the order‐disorder transition orientation of intermolecular packing, the RTP of organic molecules 2,4,6‐tris(4′‐bromo‐[1,1′‐biphenyl]‐4‐yl)‐1,3,5‐triazine (Br‐TRZ) will easily vanish upon mechanical force. Impressively, by enhancing its intramolecular charge transfer effect, the protonated Br‐TRZ stubbornly possesses an obvious RTP under external grinding, whatever in the ordered or disordered intermolecular arrangement state. Consequently, the “Lock” gate of RTP was achieved in the protonated Br‐TRZ molecule. Combined with theoretical calculation analysis, the enhanced charge transfer effect can narrow the singlet−triplet energy gap significantly, and stabilize the RTP property of triazine derivative sequentially. Furthermore, the locked RTP can be tuned via proton and counterions repeatedly and show excellent reversibility. This gated RTP concept provides an effective strategy for stabilizing the RTP emission of purely organic systems.
Neurologic Manifestations of Long COVID Differ Based on Acute COVID‐19 Severity
Authors: Perez Giraldo Gina S. , Ali Sareen T. , Kang Anthony K. , Patel Tulsi R. , Budhiraja Shreya , Gaelen Jordan I. , Lank Grace K. , Clark Jeffrey R. , Mukherjee Shreya , Singer Tracey , Venkatesh Aditi , Orban Zachary S. , Lim Patrick H. , Jimenez Millenia , Miller Janet , Taylor Carolyn , Szymanski April L , Scarpelli Jessica , Graham Edith L. , Balabanov Roumen D. , Barcelo Bianca E. , Cahan Joshua G. , Ruckman Kaitlyn , Shepard Alan G. , Slutzky Marc W. , LaFaver Kathrin , Kumthekar Priya U. , Shetty Neil K. , Carroll Katherine S. , Ho Sam U. , Lukas Rimas V. , Batra Ayush , Liotta Eric M. , Koralnik Igor J. 
Published: March 2023

ObjectiveTo characterize neurologic manifestations in post‐hospitalization Neuro‐PASC (PNP) and non‐hospitalized Neuro‐PASC (NNP) patients.MethodsProspective study of the first 100 consecutive PNP and 500 NNP patients evaluated at a Neuro‐COVID‐19 clinic between 5/2020 and 8/2021.ResultsPNP were older than NNP patients (mean 53.9 vs 44.9 y; p < 0.0001) with a higher prevalence of pre‐existing comorbidities. An average 6.8 months from onset, the main neurologic symptoms were “brain fog” (81.2%), headache (70.3%), and dizziness (49.5%) with only anosmia, dysgeusia and myalgias being more frequent in the NNP compared to the PNP group (59 vs 39%, 57.6 vs 39% and 50.4 vs 33%, all p < 0.003). Moreover, 85.8% of patients experienced fatigue. PNP more frequently had an abnormal neurologic exam than NNP patients (62.2 vs 37%, p < 0.0001). Both groups had impaired quality of life in cognitive, fatigue, sleep, anxiety, and depression domains. PNP patients performed worse on processing speed, attention, and working memory tasks than NNP patients (T‐score 41.5 vs 55, 42.5 vs 47 and 45.5 vs 49, all p < 0.001) and a US normative population. NNP patients had lower results in attention task only. Subjective impression of cognitive ability correlated with cognitive test results in NNP but not in PNP patients.InterpretationPNP and NNP patients both experience persistent neurologic symptoms affecting their quality of life. However, they harbor significant differences in demographics, comorbidities, neurologic symptoms and findings, as well as pattern of cognitive dysfunction. Such differences suggest distinct etiologies of Neuro‐PASC in these populations warranting targeted interventions. ANN NEUROL 2023;94:146–159
Astrocyte‐Derived Exosomes Contribute to Pathologies of Neuromyelitis Optica Spectrum Disorder in Rodent Model
Authors: Xie Yi , Chen Bo , Wang Qiong , Chen Xuejiao , Lai Wenwen , Xu Yaping , Deng Saiyue , Yu Zhiyuan , Xie Minjie , Bu Bitao , Mou Dapeng , Yi Chenju , Ding Fengfei , Wang Wei 
Published: March 2023

ObjectiveNeuromyelitis optica spectrum disorder (NMOSD) is an inflammatory demyelinating disease that leads to severe disability. A large proportion of NMOSD patients are seropositive for aquaporin‐4 autoantibodies (AQP4‐IgG, named as NMO‐IgG) targeting AQP4, which is selectively expressed on astrocytes in the central nervous system. This study tests the hypothesis that in response to NMO‐IgG, the pathogenic astrocyte‐derived exosomes are released and injure the neighboring cells.MethodsIgG purified from serum of either NMOSD patients or healthy controls was used to generate astrocyte‐derived exosomes (AST‐ExosNMO vs AST‐ExosCON) in cultured rat astrocytes. The exosomes were respectively delivered to cultured rat oligodendrocytes in vitro, tissue culture of rat optic nerve ex vivo, and rat optic nerve in vivo to evaluate the pathogenic roles of AST‐ExosNMO. The microRNA (miRNA) sequencing of AST‐Exos and verification were performed to identify the key pathogenic miRNA. The custom‐designed adeno‐associated virus (AAV) antagonizing the key miRNA was evaluated for its therapeutic effects in vivo. Moreover, the serum levels of the key exosomal miRNA were measured between NMOSD patients and healthy controls.ResultsAST‐ExosNMO led to notable demyelination in both cultured oligodendrocytes and optic nerve tissue. Exosomal miR‐129‐2‐3p was identified as the key miRNA mediating the demyelinating pathogenesis via downstream target gene SMAD3. AAV antagonizing miR‐129‐2‐3p protected against demyelination in an NMOSD rodent model. The serum exosomal miR‐129‐2‐3p level was significantly elevated in NMOSD patients and correlated with disease severity.InterpretationAstrocytes targeted by NMO‐IgG release pathogenic exosomes that could potentially be used as therapeutic targets or disease monitoring biomarkers in NMOSD. ANN NEUROL 2023;94:163–181
Network Architecture of Verticality Processing in the Human Thalamus
Authors: Conrad Julian , Baier Bernhard , Eberle Laurenz , Ruehl Ria Maxine , Boegle Rainer , Zwergal Andreas , Dieterich Marianne 
Published: March 2023

ObjectiveThalamic dysfunction in lesions or neurodegeneration may alter verticality perception and lead to postural imbalance and falls. The aim of the current study was to delineate the structural and functional connectivity network architecture of the vestibular representations in the thalamus by multimodal magnetic resonance imaging.MethodsSeventy‐four patients with acute unilateral isolated thalamic infarcts were studied prospectively with emphasis on the perception of verticality (tilts of the subjective visual vertical [SVV]). We used multivariate lesion–symptom mapping based on support‐vector regression to determine the thalamic nuclei associated with ipsiversive and contraversive tilts of the SVV. The lesion maps were used to evaluate the white matter disconnection and whole brain functional connectivity in healthy subjects.ResultsContraversive SVV tilts were associated with lesions of the ventral posterior lateral/medial, ventral lateral, medial pulvinar, and medial central/parafascicular nuclei. Clusters associated with ipsiversive tilts were located inferiorly (ventral posterior inferior nucleus) and laterally (ventral lateral, ventral posterior lateral, and reticular nucleus) to these areas. Distinct ascending vestibular brainstem pathways terminated in the subnuclei for ipsi‐ or contraversive verticality processing. The functional connectivity analysis showed specific patterns of cortical connections with the somatomotor network for lesions with contraversive tilts, and with the core multisensory vestibular representations (areas Ri, OP2‐3, Ig, 3av, 2v) for lesions with ipsiversive tilts.InterpretationThe functional specialization may allow both a stable representation of verticality for sensorimotor integration and flexible adaption to sudden changes in the environment. A targeted modulation of this circuitry could be a novel therapeutic strategy for higher level balance disorders of thalamocortical origin. ANN NEUROL 2023;94:133–145
Cell damage evaluation by intelligent imaging flow cytometry
Authors: Yao Yifan , He Li , Mei Liye , Weng Yueyun , Huang Jin , Wei Shubin , Li Rubing , Tian Sheng , Liu Pan , Ruan Xiaolan , Wang Du , Zhou Fuling , Lei Cheng 
Published: March 2023

AbstractEssential thrombocythemia (ET) is an uncommon situation in which the body produces too many platelets. This can cause blood clots anywhere in the body and results in various symptoms and even strokes or heart attacks. Removing excessive platelets using acoustofluidic methods receives extensive attention due to their high efficiency and high yield. While the damage to the remaining cells, such as erythrocytes and leukocytes is yet evaluated. Existing cell damage evaluation methods usually require cell staining, which are time‐consuming and labor‐intensive. In this paper, we investigate cell damage by optical time‐stretch (OTS) imaging flow cytometry with high throughput and in a label‐free manner. Specifically, we first image the erythrocytes and leukocytes sorted by acoustofluidic sorting chip with different acoustic wave powers and flowing speed using OTS imaging flow cytometry at a flowing speed up to 1 m/s. Then, we employ machine learning algorithms to extract biophysical phenotypic features from the cellular images, as well as to cluster and identify images. The results show that both the errors of the biophysical phenotypic features and the proportion of abnormal cells are within 10% in the undamaged cell groups, while the errors are much greater than 10% in the damaged cell groups, indicating that acoustofluidic sorting causes little damage to the cells within the appropriate acoustic power, agreeing well with clinical assays. Our method provides a novel approach for high‐throughput and label‐free cell damage evaluation in scientific research and clinical settings.
Policy implementation barriers in climate change adaptation: The case of Pakistan
Authors: Masud Shafaq , Khan Ahmad 
Published: March 2023

AbstractThis article focuses on the policy implementation barriers that result in poor adaptation and enhanced risk of exposure to extreme vulnerabilities, based on experiences in Pakistan. A number of policy implementation barriers are identified including: (i) Whether policy development is seen as a closed or open consultation process, (ii) Whether policy is seen as a generic document or an instrument for specific actions, and (iii) Whether policy administration is seen as a central or devolved process. As a case study, Pakistan's national climate change policy was considered. Pakistan is the eighth most climate change impacted country in the world. The results conclude that a prevalent top‐down frame of management, centred‐around federalism, has created vacuums for misinterpretation, poor understanding amongst policy implementers and false expectations of policy goals. A critical aspect of this disconnect is the lack of inclusivity of critical stakeholder groups in the policy design and development stages thus creating various barriers resulting in mistrust between different governing entities and vice versa. The study also highlights the nature of this problem and recommends a closer examination of the prerequisites of engagement in climate decision‐making for better understanding and implementation of adaptation practices in de‐centralised governance.
Influence of energy storage system in nanogrid under dynamic conditions with frequency‐based current controller for battery storage
Authors: Soni Yuvraj Praveen , Fernandez Eugene 
Published: March 2023

AbstractMaintaining a stable voltage level at the DC bus is crucial to safeguard equipment, ensure a consistent power supply, and enhance system efficiency. A combination of battery storage and photovoltaic (PV) systems is often employed to maintain an equilibrium between energy supply and demand and keep the DC bus voltage within optimal levels. Nonetheless, frequent and rapid charging and discharging of battery storage in dynamic conditions can negatively impact its lifespan. To address this issue, this study utilizes the application of a frequency‐based current controller in battery storage that facilitates smooth charging and discharging operations in transient conditions. The study evaluates the performance of a grid‐tied PV‐based nanogrid (GT‐PVN) system with three distinct configurations: (a) PV system without storage, (b) PV system with battery storage, and (c) PV system with hybrid energy storage system (HESS) under varying dynamic load and irradiance conditions. The results show that the transient performance on DC bus voltage with battery integration is efficiently improved by 0.3%‐0.5% under dynamic irradiance conditions and 0.5%‐1.17% under dynamic load conditions. Further, with HESS installation, the DC bus voltage transients are improved by 0.85%‐1.25% under dynamic irradiance conditions and 0.5%‐1.5% under dynamic load conditions. Moreover, energy storage technology implementation has the potential to decrease energy procurement from the grid by up to 80%. These findings highlight the potential of energy storage technology in mitigating the intermittency and dynamic operational challenges faced by PV systems.
Lidar visualization techniques for the construction of geoarchaeological deposit models: An overview and evaluation in alluvial environments
Authors: Crabb Nicholas , Carey Chris , Howard Andy J. , Brolly Matthew 
Published: March 2023

AbstractLidar has become an essential tool for the mapping and interpretation of natural and archaeological features within the landscape. It is also increasingly integrated and visualized within geoarchaeological deposit models, providing valuable topographic and stratigraphic control from the contemporary ground surface downwards. However, there is a wide range of methods available for the visualization of lidar elevation models and a review of existing research suggests that it remains unclear which are most appropriate for geoarchaeological applications. This paper addresses this issue by providing an overview and quantitative evaluation of these techniques with examples from archaeologically resource‐rich alluvial environments. Owing to the relatively low‐relief nature of the terrain within these temperate lowland flood plain environments, the results show that there is a small number of visualization methods that demonstrably improve the detection of geomorphological landforms that can be related to the variable distribution of archaeological resources. More specifically, a combination of Relative Elevation Models combined with Simple Local Relief Models offered an optimal approach that subsequently allows integration with deposit models. Whilst the presented examples are from a flood plain setting, deposit models are pertinent to a range of landscape contexts and the methodology applied here has wider applicability.
Discrimination between normal and necrotic small intestinal tissue using hyperspectral imaging and unsupervised classification
Authors: Zhang Lechao , Huang Danfei , Chen Xiaojing , Zhu Libin , Xie Zhonghao , Chen Xiaoqing , Cui Guihua , Zhou Yao , Huang Guangzao , Shi Wen 
Published: March 2023

AbstractObjective and automatic clinical discrimination of normal and necrotic sites of small intestinal tissue remains challenging. In this study, hyperspectral imaging (HSI) and unsupervised classification techniques were used to distinguish normal and necrotic sites of small intestinal tissues. Small intestinal tissue hyperspectral images of eight Japanese large‐eared white rabbits were acquired using a visible near‐infrared hyperspectral camera, and K‐means and density peaks (DP) clustering algorithms were used to differentiate between normal and necrotic tissue. The three cases in this study showed that the average clustering purity of the DP clustering algorithm reached 92.07% when the two band combinations of 500–622 and 700–858 nm were selected. The results of this study suggest that HSI and DP clustering can assist physicians in distinguishing between normal and necrotic sites in the small intestine in vivo.
Binding mechanism of anacardic acid, carnosol and garcinol with PCAF: A comprehensive study using molecular docking and molecular dynamics simulations and binding free energy analysis
Authors: Jaganathan Ramakrishnan , Kumaradhas Poomani 
Published: March 2023

AbstractThe p300/CBP associated factor bromodomain (PCAF Brd) is emerged as one of the promising target proteins for different types of cancers. PCAF is one among the histone acetyltransferase enzymes which involved in the regulation of transcriptase process by modifying the chromatin structure. Anacardic acid, carnosol, garcinol are the experimentally reported inhibitors of PCAF Brd; however, their detailed binding mechanism these inhibitors are not yet known. The intermolecular interaction, binding energy, and the stability of these inhibitors with the active site of PCAF Brd are playing the key role in the binding of these inhibitors with PCAF. The in silico study incorporates the molecular docking and dynamics simulations; these molecular level simulations allow to understand the binding mechanism. In the present study, the induced fit molecular docking and molecular dynamics of anacardic acid, carnosol and garcinol molecules against the PCAF Brd have been performed. The docking score values of these molecules are −5.112 (anacardic acid), −5.141 (carnosol), −5.199 (garcinol) and −3.641 (L45) kcal/mol, respectively. Further, the molecular dynamics simulation was carried out for these docked complexes to understand their conformational their stability and binding energy from the roots means square deviation (RMSD) and root means square of fluctuation (RMSF), and molecular mechanics with the generalized born and surface area solvation (MM/GBSA) binding free energy calculations. The intermolecular interactions and binding free energy values confirm that garcinol forms key interactions and has high binding affinity towards PCAF Brd on compare with the other two inhibitors. Therefore, garcinol may be considered as a potential inhibitor of PCAF Brd.