bluuebunny/crossref_metadata_2025_split huggingface dataset:
Cognitive Agent based Data Processing in Ubiquitous Network using Reinforcement Learning Approach
Authors: Bhajantri Lokesh B. , Ayyanavar Vasudha V. 
Published: January 2025

Abstract

In the present era of heterogeneous networks, the data will be gathered from various diverse sources with varying data formats. Sometimes the data is used to make immediate decision making, such as adjusting environmental controls based on sensors or ubiquitous devices inputs or triggering alerts of devices in response to certain events. Hence, data processing is an essential task to contextualize information by analyzing various data points together. By processing data efficiently, UNT can optimize resource usage. The proposed work aims to tackle the problems of processing both real-time and non-real-time using a Cognitive Agents based machine learning approach for predictive analysis for the processing of data and evaluated against existing methods, comparing factors such as data processing time, query response time, energy consumption, throughput and computation overhead.
Ecological Determinants of Chital (Axis axis) Reproductive Seasonality in Kanha Tiger Reserve
Authors: Goswami Shravana , Kumar Ujjwal , Qureshi Qamar , Jhala Yadvendradev 
Published: January 2025

Abstract

Reproductive seasonality in mammals is often influenced by environmental factors like resource availability, predation. While tropical and equatorial regions exhibit continual breeding due to stable resources, tropical India experiences pronounced seasonality in forage availability due to monsoonal rainfall. This study investigates the breeding timing of chital (Axis axis) in the tropical deciduous forests of Central India at Kanha Tiger Reserve, where forage availability varies seasonally. Field observations focused on antler conditions in males and lactation signs in females to estimate breeding timing. Rutting peak, as determined by males with hard antlers, coincided with nutritional peak induced by monsoon, which probably was the cue for ovulation in females. Lactation, the most nutritionally demanding period, peaked with the post-fire sprouting of herbaceous vegetation in April and fawn weaning synchronized with ample food availability. This synchrony between reproductive cycles and forage availability emphasizes the adaptive strategies of chital to maximize offspring survival. Furthermore, the peak in tiger births (54%) coincided with the fawning peak of chital. making them vulnerable prey during a nutritionally demanding period for tigresses, who are restricted in their movements near vulnerable cubs. Understanding the links between trophic levels and their seasonality offers important insights for wildlife management and conservation strategies.
Association between Nonsugar Sweetener Intake and Kidney Stone Among US Adults: Cross-sectional Results from NHANES 2007- 2018
Authors: Leng Wenyuan , Li Xiaoyu , Zhu Zhenpeng , Gao Duan , Hu Weimin , Xu Chunru , Wang Gang , Lin Jian 
Published: January 2025

Abstract

Backgroud:
Kidney stones, a prevalent urological condition, impose a significant social burden. While some unhealthy dietary habits are known risk factors, the relationship between nonsugar sweetener (NSS) intake and kidney stone incidence remains unexplored.
Methods
Data from the National Health and Nutrition Examination Survey (NHANES) 2007–2018 were analyzed. NSS intake was calculated from the first 24-hour dietary recall. Logistic regression, stratified analysis, and interaction analysis were used to explore associations between NSS intake and kidney stone incidence. Probability prediction plots visualized these relationships.
Results
In total, 6,891 U.S. residents aged 20 and above with complete data were included. The overall incidence of kidney stones was 11.62%, with rates of 12.17% (Q1), 11.04% (Q2), 11.35% (Q3), and 10.68% (Q4). After adjusting for covariates, high NSS intake showed a protective effect against kidney stone incidence (Q3 group, OR = 0.1101, 95% CI: 0.0129–0.9388, P = 0.0438). However, family income and extreme BMI levels modified this protective effect. For individuals with diabetes and coronary heart disease, high NSS intake increased the risk of kidney stones.
Conclusions
High NSS intake may protect against kidney stones in U.S. adults, but this effect varies with income and BMI levels. However, for those with diabetes or coronary heart disease, high NSS intake increases the risk. Validation through large-scale cohort studies is necessary.
Association of alkaline phosphatase and calcium levels in serum with hyperuricemia and hyperglycemia: a cross-sectional study among adults living in cold region of China
Authors: Jiang Lan , Dong Jing , Zou Lina , Ren Huanyu , Li Zheng , Niu Huiru , Liao Hao , Zhang Xiaojing , An Shanshan , Ren Fei , Ge Xiuhong , Cheng Lang , Yang Feiyan , Pan Hongzhi , Rong Shengzhong , Gao Peng , Ma Hongkun 
Published: January 2025

Abstract

Objective
Alkaline phosphatase (ALP) and calcium (Ca) levels are common indexes in serum biochemical tests, but their relationship with HUA and hyperglycemia is still unclear. The objective of this study was to investigate the relationship of ALP and Ca levels with HUA and hyperglycemia in cold region of China.
Methods
Baseline data were collected via questionnaires, and blood samples were analyzed biochemically. Spearman correlation and multiple linear regression examined associations between ALP, Ca, serum uric acid (SUA), and fasting blood glucose (FPG). A binary logistic regression model with ALP and Ca predicted HUA and hyperglycemia risk.
Results
A total of 340 subjects were included in this study. Based on the diagnostic criteria for HUA and hyperglycemia, patients were classified into a HUA group (n = 100) and a non-HUA group (n = 240), as well as a hyperglycemia group (n = 59) and a non-hyperglycemia group (n = 281).The serum levels of ALP and Ca in the HUA group were significantly higher than those in the control group; similarly, the ALP levels in the hyperglycemia group were also significantly higher than those in the control group. Binary logistic regression showed a higher risk of HUA and hyperglycemia in Q4 compared to Q1 after stratifying by ALP and Ca ( ORs > 1, 95%CIs > 1, P < 0.05). The combined ALP and Ca had superior diagnostic performance for HUA, while ALP alone outperformed other indicators for hyperglycemia diagnosis (P < 0.001).
Conclusions
In populations residing in cold regions of China, an increase in ALP and Ca levels may elevate the risk of HUA and hyperglycemia onset.
Association between co-residence with parents and General Anxiety Disorder in Korean adolescents
Authors: Kim Kyung-cheol , Yoon Seong-mo , Song Seung-won , Park Su-Min , Park Eun-Cheol 
Published: January 2025

Abstract

Background
Generalized anxiety disorder (GAD) is a significant mental health concern, especially among Korean adolescents, who exhibit a heightened vulnerability to anxiety due to psychological, physiological, and social factors. Elements such as family dynamics, social pressures, and academic demands constitute substantial risks to adolescent mental health. Additionally, the shift toward nuclear family structures and economic challenges, which have reduced parental time with adolescents, have been identified as contributing factors to this issue. This study examined whether the association between parental co-residence and adolescent anxiety is warranted.
Methods
The study data were sourced from the 2020–2023 Youth Health Behavior Survey by the Korea Disease Control and Prevention Agency (sample of 208,891 Korean adolescents aged 13 to 18 years). Adolescent anxiety levels were measured using the Generalized Anxiety Disorder-7 (GAD-7) screening tool. To investigate the association between parental co-residence and GAD, multiple logistic regression analyses were conducted.
Results
Adolescents who were not residing with their parents had more anxiety compared to those residing with parents (Male: OR: 1.19, 95% CI: 1.10–1.28; Female: OR: 1.08, 95% CI: 1.00–1.17). Notably, adolescents co-residing with relatives had the most anxiety (Male: OR: 1.67, 95% CI: 1.35–2.08; Female: OR: 1.31, 95% CI: 1.05–1.64).
Conclusion
The study found that adolescents who were not residing with their parents faced an increased risk of anxiety. These adolescents therefore may require anxiety reduction-oriented care.
Computational and Machine Learning Approaches for Optimizing Anti-CD3ε Nanobody: Humanization and Characterization for Enhanced Therapeutic Efficacy
Authors: Bonab Ali Rahmati , Ghahi Hannaneh Jalilzadeh , Hassani Mahmoud , Jajarmi Vahid , Ranjbari Javad 
Published: January 2025

Abstract

This study presents a comprehensive machine-learning-driven approach for the in silico humanization and characterization of anti-CD3ε nanobodies. Nanobodies, single-domain antibodies derived from camelids, hold immense therapeutic potential due to their small size, high solubility, and exceptional stability. However, their camelid origin necessitates humanization to minimize immunogenicity in therapeutic applications. Using state-of-the-art computational tools such as NanoNet, RoseTTAFold, and PyDock, we modeled and analyzed both wild type and humanized anti-CD3ε nanobody variants. Key metrics, including structural stability, binding efficiency, thermal stability, and aggregation propensity, were evaluated. Humanization achieved enhanced humanness scores, increased thermal stability, and retained strong binding interactions with CD3ε while preserving the nanobody’s structural integrity. Molecular dynamics simulations confirmed minimal deviations in structural flexibility and binding-site compatibility post-humanization. These findings support the efficacy of computational methods in optimizing nanobody therapeutics for clinical applications, paving the way for advanced immunotherapy strategies targeting immune-related disorders. The results demonstrate that the humanized anti-CD3ε nanobody exhibits enhanced thermal stability, reduced aggregation propensity, improved humanness scores, and comparable binding efficiency to the wild type nanobody, making it a promising therapeutic candidate.
Wild boars as potential reservoirs of Ascaris lumbricoides: Molecular evidence of a novel transmission route
Authors: Matsumura Takahiro , Mochizuki Kota , Matsuo Kayoko , Komiya Tomoyoshi 
Published: January 2025

Abstract

Background
The genus Ascaris includes A. lumbricoides, which primarily infects humans as its definitive host, and A. suum, which primarily infects pigs. Owing to their morphological similarities, the ability to distinguish between these two species in reported cases of ascariasis has been reliant on molecular techniques, particularly differences at the 133rd and 246th nucleotide positions of the internal transcribed spacer 1 (ITS-1) region. Although Ascaris nematodes have also been detected in wild boars, they are often classified as A. suum without molecular confirmation because of the close genetic relationship between wild boars and pigs. To address this gap, we conducted a molecular analysis targeting the ITS-1 region to identify the species of Ascaris worms collected from six wild boars captured in Ishikawa Prefecture, Japan.
Results
Sequence alignment analysis revealed a deletion at nucleotide position 129, a G at position 133, and a T at position 246. This configuration showed 100% identity with the A. lumbricoides reference sequence previously identified in humans. On the basis of these findings, the samples were classified as A. lumbricoides.
Conclusions
This study provides the first molecular evidence that A. lumbricoides, which was previously thought to be nearly eradicated in Japan, persists in wild boar populations. While infections with A. lumbricoides in Japan are currently believed to be limited to imported cases involving travelers and foreign residents, sporadic cases of unknown origin have been reported among Japanese individuals. Traditionally, potential infection sources have included organic vegetables, imported produce with contaminated soil, and imported kimchi. However, the detection of A. lumbricoides in wild boars highlights a possible zoonotic transmission route that has not been previously considered. This finding underscores the need for further research into wild boars as potential reservoirs of A. lumbricoides and the implementation of preventive strategies to mitigate the associated risks.
Trial registration
Not applicable.
Correlation Analysis of the Pindicator of PNI,SII of Reoperative Immunological and Postoperative Delirium in Patients Undergoing Thoracoscopic Surgery
Authors: Wang Yang , Wang Zhao , Wang Huazhen , Wang Shiyan , Bi Hehui , Liu Jiuyue , Jin Xianglan 
Published: January 2025

Abstract

Objective: To explore the relationship between preoperative nutritional index (PNI) and systemic immune inflammatory index (SII) levels and postoperative delirium (POD) in patients undergoing thoracoscopic surgery, and explore the risk factorsfor POD during thoracoscopic surgery.
Methods: This study selected 106 patients who underwent thoracoscopic surgery under general anesthesia at a tertiary hospital. One day before surgery, the Mini Mental State Evaluation Scale (MMSE) was used to evaluate the preoperative cognitive function of surgical patients. The results of the patient's last preoperative laboratory examination were recorded, and preoperative PNI and SII levels were calculated. On the first to third day aftersurgery, four rapid delirium diagnosis protocols (4AT) were used daily to evaluate the occurrence of POD in patients. Based on whether POD occurred, patients were divided into delirium group (POD group) and non delirium group (NPOD group), with 23 casesin the POD group and 65 casesin the NPOD group. Observing the incidence of POD after thoracoscopic surgery, comparing the clinical characteristics and preoperative laboratory test results of two groups of patients, using multivariate logistic regression method to analyze the independent risk factors for POD in patients, drawing receiver operating characteristic (ROC) curves, calculating the product under the ROC curve (AUC), and analyzing the predictive value of preoperative PNI and SIIlevelsfor POD in thoracoscopic surgery patients.
Results: 1. The preoperative PNI levels of patients in the POD group were significantly lower than those in the NPOD group (P < 0.05), while the preoperative SII levels of patients in the POD group were significantly higherthan those in the NPOD group (P < 0.05).
2. preoperative PNI and SII levels have certain predictive value for POD in patients undergoing thoracoscopic surgery.
Conclusions: Increasing age, decreasing preoperative PNI level and increasing preoperative SII level were independent risk factors and effective predictors of POD in patients undergoing thoracoscopic surgery.
The virulence factor LLO of Listeria monocytogenes can slow down biofilm formation and indirectly suppress phage lytic effect
Authors: Liu Banhong , Bai Mei , Tu Wuxiang , Shen Yanbin , Liu Jingxin , Yang Zhenquan , Bao Hongduo , Wang Ran , Dong Qingli , Liu Yangtai , Zhang Hui , Hu Liangbing 
Published: January 2025

Abstract

Phage-based biocontrols are among the various tools available to control Listeria monocytogenes (Lm) in food and food processing environments. However, the effectiveness of phages against different Lm strains varies significantly. Prior research has demonstrated that the inactivation of virulence Listeriolysin O (LLO) leads to a loss of hemolytic activity and a reduction in overall virulence. In this study, we generated a hly gene-defective strain, NJ05-Δhly, which demonstrated a significant enhancement in motility despite the absence of hemolytic activity. Furthermore, NJ05-Δhly displayed diminished capacity for biofilm formation, which negatively correlated with glucose concentration. The efficiency of plating (EOP) for the defective strain decreased by approximately 100-fold following infection with Listeria phage vB-LmoM-NJ05, with a notable reduction in lytic effect observed at multiplicities of infection (MOI) of 1 or 10. With 108 PFU/mL of phage treatment, the biofilm of Lm NJ05-Δhly was completely suppressed and removed. Moreover, phage treatment effectively decreased adhesion and invasion, as well as mitochondrial membrane damage, in addition to the accumulation of mitochondrial reactive oxygen species (ROS), even in the absence of LLO. Transcriptome analysis indicated that LLO is mainly associated with pathways involved in quorum sensing, purine metabolism, and peptidoglycan biosynthesis, among others. Notably, genes related to Lm phage sensitivity, pathogenicity, biofilm formation, and motility were significantly down-regulated. These findings suggest that LLO plays a crucial role in regulating Lm pathogenicity, phage susceptibility, and biofilm formation. This study provides evidence of the complex interplay between Listeria LLO and phage interactions during Lm infection.
Diagnosing Acute Appendicitis in Children with Neutrophil-Lymphocyte Ratio: A Cross-Sectional Study Running title: Diagnosing AA in Children with NLR
Authors: Alawi Ahmed , Fatani Bayan , Alsaggaf Ameen , Ghallab Alaa , Awad Mohammed , Shalaby Mohamed 
Published: January 2025

Abstract

Purpose
This study aimed to evaluate the value of neutrophil-to-lymphocyte ratio (NLR) in diagnosing AA in the pediatric population.
Methods
This retrospective cross-sectional study involved 200 pediatric patients aged between 0–14 years of both sexes, admitted as a case of acute appendicitis (AA). Data of patients retrieved from the hospital's patient administration system database. Normal appendex group (n = 100) included patients with abdominal pain without appendicitis. Uncomplicated AA group (n = 165) included patients with abdominal pain diagnosed with simple AA and treated with medical treatment or simple appendectomy. Complicated AA group (n = 35) included patients with recurrent or complicated appendicitis.
Results
White blood cells (WBCs), neutrophils and NLR were significantly higher in the uncomplicated and complicated appendicitis groups than the normal appendix group and in the complicated appendicitis group than the uncomplicated appendicitis group. In multivariate regression, WBCs and NLR were independent predictors for AA (P ≤ 0.001). NLR was an independent predictor for complicated appendicitis (P = 0.012). NLR can significantly predict AA and complicated appendicitis repectively at cut-off > 1.7 and > 10.1 with 74% and 68.57% sensitivity, and 69% and 56.98% specificity.
Conclusions
NLR is a valuable and cost-effective diagnostic marker for AA and complicated appendicitis in the pediatric population.
Identification and clinical implications of the immune-related hub genes in psoriasis
Authors: Sun Yuzhen , Mao Yu , Liu Niu , Li Yanfeng , Zhou Ziguang , Fang Weiyuan 
Published: January 2025

Abstract

Methods: Multiple microarray datasets from psoriasis patients (GSE30999, GSE106992, GSE14905, GSE78097, and GSE117468) were obtained to identify immune-key genes by differential gene analysis and Weighted Gene Co-expression Network Analysis (WGCNA). Subsequently, immune-related hub genes were identified using the Least Absolute Shrinkage and Selection Operator (LASSO) algorithm and Protein-Protein Interaction (PPI) networks, with further validation through Gene Set Enrichment Analysis (GSEA) and Receiver Operating Characteristic (ROC) curves to assess diagnostic potential. Pearson correlation analysis evaluated the relationship between hub genes, skin lesion severity, and treatment outcomes. The study also conducted immune infiltration by using the Cell-type Identification By Estimating Relative Subsets Of RNA Transcripts (CIBERSORT) algorithm and identified potential therapeutic targets by the Drug-Gene Interaction Database (DGIdb).
Culturally appropriate, evidence-based, personalized dietary advice to improve the nutritional status of track and field athletes: a randomized controlled trial
Authors: Jayawardena Ranil , Weerasinghe Kalani , Nanayakkara Indu , Madhujith Terrence , Hills Andrew P , Kalupahana Nishan Sudheera 
Published: January 2025

Abstract

Background: Optimal nutrition is vital for recovery, performance, and well-being of athletes. However, Sri Lankan track and field athletes often fall short of dietary recommendations due to the lack of personalized nutrition guidance. This study evaluated the effectiveness of culturally appropriate, evidence-based, personalized dietary advice on the nutritional status of these athletes.
Methods: This 16-week parallel-group randomized controlled trial included 30 national-level track and field athletes (15 in the intervention group [IG], 15 in the control group [CG]). The IG received personalized dietary prescriptions based on detailed nutritional assessments, while the CG received no advice. Outcomes included dietary intake, assessed via seven-day food diaries, and biochemical parameters measured using standard techniques.
Results: Twenty-seven athletes (IG: 13, CG: 14) completed the study. The IG showed significant increases in energy intake (Pre: 2766.0 ± 494.0 kcal, Post: 3972.9 ± 934.4 kcal, Change: +1206.9 kcal; p = 0.007) compared to the CG (Pre: 2733.7 ± 635.5 kcal, Post: 2676.5 ± 319.2 kcal, Change: -57.2 kcal; p = 0.739; p for IG vs. CG = 0.004). Protein intake also improved significantly in the IG (Pre: 95.56 ± 30.2 g, Post: 116.81 ± 41.8 g, Change: +21.25 g; p = 0.003) versus the CG (Pre: 94.54 ± 32.8 g, Post: 84.24 ± 16.4 g, Change: -10.30 g; p = 0.162; p for IG vs. CG = 0.001). The IG exhibited improved vitamin and mineral intakes, and serum vitamin D levels in the IG increased significantly (Pre: 30.55 ± 10.2 ng/ml, Post: 42.02 ± 9.6 ng/ml; Change: +11.47 ± 0.6 ng/ml; p < 0.001), while the CG showed no change. Serum ferritin levels also increased in the IG (Pre: 44.76 ± 29.3 ng/ml, Post: 51.54 ± 28.4 ng/ml, Change: +6.78 ± 0.9 ng/ml; p < 0.001), along with positive changes in haemoglobin and other haematological parameters.
Conclusions: A culturally appropriate, personalized dietary prescription significantly improved the nutritional status among Sri Lankan track and field athletes.
Mean Arterial Pressure (MAP) influence on free flap oxygen saturation (StO2) measured by Near-Infrared Spectroscopy
Authors: Gilli Massimiliano , Committeri Umberto , Monarchi Gabriele , sacchi federica , cirignaco giulio , caso angela rosa , giovacchini francesco , mitro valeria , tullio antonio 
Published: January 2025

Abstract

Background
Free flap monitoring techniques still rely on gold standard methods such as skin color evaluation, temperature, and puncture tests that are time-consuming, discontinuous, and prone to human error. Near-Infrared Spectroscopy represents a continuous, non-invasive technique that aims to avoid subjective errors by detecting early stages of vascular compromise, providing enough time for successful salvage surgery.
Methods
This retrospective study includes 56 patients undergoing microvascular reconstruction and 24-hour NIRS monitoring. All other patients without detailed MAP charts were excluded. The NIRS system used was the HemoSphere-ForeSight by Edwards Lifesciences; this platform enabled the detection of StO2 values and their integration with real-time intra-arterial catheter-detected MAP values. Data were recorded by an adhesive sensor, chosen according to the height and width of the flap.
Results
Forty-seven (83%) patients showed a positive correlation between MAP and StO2 data (ρs > 0.450, p < 0.0001), validating the theory that MAP values positively correlate with flap perfusion, tissue oxygenation, and survival rate. Nine patients did not show a direct correlation between MAP and StO2 data; their values were influenced by vasopressors or psychophysical conditions during their admission to the Intensive Care Unit (ICU) after surgery.
Conclusion
Comparing MAP and StO2 values, their trend charts play a crucial role in flap monitoring, contributing to the best possible survival rate outcome and, if necessary, leading to micro-anastomosis checks and eventual flap salvage surgery. MAP data analysis should, indeed, be considered in future guidelines for free flap monitoring techniques.