bluuebunny/crossref_metadata_2025_split huggingface dataset:
НАУКОВА ПОЛЕМІКА ЩОДО ТАКТИЧНИХ ОПЕРАЦІЙ СТОСОВНО ЗБИРАННЯ ПОЧАТКОВИХ ВІДОМОСТЕЙ ПРО ОБСТАВИНИ ПОДІЇ ТА ВИЯВЛЕННЯ ОЗНАК ШАХРАЙСТВА У СФЕРІ ВИКОРИСТАННЯ БАНКІВСЬКИХ ЕЛЕКТРОННИХ ПЛАТЕЖІВ
Authors: Вискарка Т. В. 
Published: January 2025

Наукова стаття присвячена деяким аспектам розслідування шахрайства у сфері використання банківських електронних платежів. Зазначено, що на часі в криміналістиці існує така наукова категорія як «тактичні операції». З іншого боку, в загальній теорії методики розслідування кримінальних правопорушень вказаний інструмент майже не зустрічається. В свою чергу, ми вважаємо його найбільш оптимальним для кримінальних проваджень щодо випадків вчинення шахрайства у сфері використання банківських електронних платежів. Адже шахраї в досліджуваній категорії кримінальних проваджень мають високий інтелектуальний розвиток та комп’ютерну грамотність. Тому будь-яке зволікання в процесі реалізації вказаних дій може призвести до знищення важливої доказової інформації (віртуальної, матеріальної чи ідеальної). В той час, як реалізація відповідних тактичних операцій максимально убезпечить працівників правоохоронних органів від зазначених негативних наслідків. Визначено, що тактична операція – це сукупність слідчих (розшукових) дій, негласних слідчих (розшукових) дій та інших процесуальних заходів, що вирішують конкретне тактичне завдання, яке має безпосереднє значення для ефективного здійснення певного кримінального провадження. Сформульовано завдання, які необхідно вирішити в ході реалізації тактичних операцій стосовно збирання початкових відомостей про обставини події та виявлення ознак шахрайства у сфері використання банківських електронних платежів, зокрема: 1) умови, які передували вчиненню шахрайських дій; 2) конкретна електронно-обчислювальна техніка, за допомогою якої реалізувалися шахрайські дії; 3) кількість фактів протиправної діяльності; 4) час вчинення шахрайства, а також тривалість вказаних дій; 5) кількість потерпілих, які постраждали внаслідок дій шахраїв; 6) способи шахрайських дій (фішинг, кардинг, сніферинг); 7) учасники вчинення шахрайства, їх кількість та роль кожного; 8) особи, які можуть надати криміналістично-значиму інформацію про обставин вчинення шахрайства та осіб, які до цього причетні; 9) збір інших даних стосовно події та джерел криміналістично значимої інформації.
PROCESSOS DE ENSINO E APRENDIZAGEM NOS ANOS INICIAIS E AS TECNOLOGIAS DIGITAIS
Authors: Jucemar dos Santos Priscila , Dias de Oliveira Eduarda , Corrêa Shirlei de Souza , Zluhan Mara Regina , Alves Cleide 
Published: January 2025

O presente estudo busca refletir acerca das práticas metodológicas atuais nos Anos Iniciais do Ensino Fundamental e no seu papel de romper com fronteiras puramente técnicas, para avançar na formação de um aluno reflexivo, crítico, ético e transformador. Com o objetivo de discutir a importância da utilização dos recursos tecnológicos nas práticas pedagógicas dos Anos Iniciais do Ensino Fundamental. Foi utilizada como metodologia a pesquisa documental e bibliográfica. Constata-se como o processo ensino ainda está focado na metodologia tradicional, que desestimula a vontade de aprender, já que não atende mais às necessidades dos alunos neste novo tempo. Além disso, há desafios internos e externos que o discente enfrenta, podendo ser um agravante na aprendizagem.
Palavras chave: Tecnologias digitais. Práticas pedagógicas. Pandemia.
Abstract
The present study seeks to reflect on current methodological practices in the Initial Years of Elementary School and its role in breaking with purely technical boundaries, to advance in the formation of a reflective, critical, ethical and transformative student. With the aim of discussing the importance of using technological resources in pedagogical practices in the Initial Years of Elementary School. Documentary and bibliographical research was used as a methodology. It can be seen how the teaching process is still focused on traditional methodology, which discourages the desire to learn, as it no longer meets the needs of students in this new era. Furthermore, there are internal and external challenges that the student faces, which can be an aggravating factor in learning.
Keywords: Digital technologies. Pedagogical practices. Pandemic.
Impacts of surface wear of attachments on maxillary canine distalization with clear aligners: a three-dimensional finite element study
Authors: Li Qiuying , Xu Bowen , Fang Dongyu , Yang Kai 
Published: January 2025

ObjectivesThis study established three-dimensional finite element models to explore the impacts of surface wear of attachments on maxillary canine distalization with clear aligners, thereby guiding the clinical application of attachments and enhancing the efficiency of clear aligner therapy.Materials and methodsFinite element models of maxillary canine distalization, including the maxilla, dentition, periodontal ligament, attachments (in both initial and worn states), and clear aligners, were established. Two groups of attachments (vertical rectangular attachment and optimized root control attachment) and five working conditions representing different degrees of attachment wear (M0, M2, M4, M6, and M8) were designed for canine distalization. Tooth displacement and equivalent stress in the roots and periodontal ligaments were analyzed.ResultsThe canines in both groups exhibited a tipping movement pattern under all working conditions. By M8, the distal displacement of the canine crown, the equivalent stress values in the roots, and the equivalent stress values in the periodontal ligaments in the rectangular attachment group decreased by 12.04%, 30.80%, and 16.48%, respectively, compared to M0. In the optimized root control attachment group, these values decreased by 24.98%, 34.69%, and 19.15%, respectively. However, under all working conditions, the canines in the rectangular attachment group presented greater displacement and stress. The greatest reduction in canine crown distal displacement and stress values was observed between M6 and M8 in the rectangular attachment group, but the efficiency of canine distalization was still 64.30% at M8, with minimal change. In the optimized root control attachment group, the greatest reduction was observed in M4–M6, and the efficiency of canine distalization decreased to less than 60% in response to M6.ConclusionThe canines tended to tip when maxillary canine distalization was performed with clear aligners. Attachment wear led to a reduction in the efficiency of canine distalization. Compared with optimized root control attachments, the impact was less significant for rectangular attachments. Once optimized root control attachments have been in place for more than 4 months and maxillary canine distalization is still required, orthodontists should closely monitor the wear of these attachments. If necessary, timely restoration or rebonding of the attachments is recommended.
Interpreting IGF-1 in children treated with recombinant growth hormone: challenges during early puberty
Authors: Ly Helena-Jamin , Ankarberg-Lindgren Carina , Fors Hans , Nilsson Staffan , Dahlgren Jovanna 
Published: January 2025

ObjectiveIt can be challenging to determine the correct dosage of recombinant growth hormone (GH) in children with GH deficiency, leading to highly variable treatment responses. Insulin-like growth factor-1 (IGF-1) is a tool for monitoring GH treatment and dosing. However, IGF-1 levels depend on sex, age, and pubertal stage, amongst other factors, making its interpretation somewhat difficult. This study aimed to evaluate descriptively a group of 93 children treated per protocol with GH to assess the influence of pubertal signs and sex steroid levels on the interpretation of IGF-1.Methods93 (67 boys and 26 girls) prepubertal children who participated in a previous GH treatment trial were included. Age, pubertal stage, weight, height, GH dose, and IGF-1 plasma concentrations were collected at least yearly from 2 years before pubertal start and 3 years after pubertal start. Levels of estradiol in girls and testosterone in boys were analyzed from previously collected frozen samples.ResultsNine of 58 (15.5%) estradiol samples in girls with Tanner breast stage 1 had pubertal levels of estradiol ≥25 pmol/L. For boys with testes size &lt;4 mL, 24 out of the 153 (15.7%) testosterone samples were above the pubertal cut-off, ≥0.47 nmol/L. All the IGF-1 samples were divided into two groups based on an IGF-1 standard deviation score (SDS) of ≥2 or &lt;2 SDS. The IGF-1 ≥2 SDS samples had a higher median (range) GH dose, 0.042 (0.02-0.10) mg/kg/day, compared with the IGF-1 &lt;2 SDS samples, 0.038 (0.01-0.10) mg/kg/day, p&lt;0.001. In the IGF-1 ≥2 SDS samples vs the IGF &lt;2 SDS samples, estradiol levels were lower among girls, 13 (3-214) vs 102 (1-1070) pmol/L p&lt;0.001, and testosterone levels were lower among boys, 0.35 (0.11-27.2) vs 6.9 (0.04-31.2) nmol/L p&lt;0.001.ConclusionInterpretation of IGF-1 near puberty is challenging due to the influence of sex steroids. Variations in sex steroid levels and pubertal status can lead to misleading interpretations and an overestimation of IGF-1 SDS. Establishing an IGF-1 reference range that includes sex steroid levels can improve its clinical use to monitor GH treatment.
Assessment of hypoxia status in a rat chronic liver disease model using IVIM and T1 mapping
Authors: Dong Wenlu , Xiao Longyang , Luo Ziwei , Yu Haiyang , Wang Lili , Gao Yuanxiang , Li Zhiming 
Published: January 2025

ObjectivesThis study was aimed to assess the diagnostic performance of intravoxel incoherent motion (IVIM) magnetic resonance imaging (MRI) and T1 mapping in detecting hypoxia status of chronic liver disease using a carbon tetrachloride (CCl4)-induced rat model.Materials and methodsThe hypoxia group of chronic liver disease consisted of eight rats induced by injection of CCl4 and the control group consisted of nine rats injected with pure olive oil. All 17 rats underwent MRI examination at week 13 after injection, using T1 mapping and IVIM. Liver specimens were subjected to immunohistochemical staining for the exogenous hypoxia marker pimonidazole and the endogenous hypoxia marker HIF-1α and scored semi-quantitatively. Differences in MRI multiparameters, pimonidazole H-scores, and HIF-1α were analyzed between the control and hypoxia groups. Correlations between MRI multiparameters and H-score, and MRI multiparameters and HIF-1α, were analyzed, and the diagnostic performance of multiparameter MRI was evaluated by receiver operating characteristic (ROC) curve analysis.ResultsThere were significant differences between the control group and the hypoxia group in D* values (p = 0.01) and f values (p = 0.025) of IVIM parameters, T1 mapping (p = 0.003), HIF-1α (p &lt; 0.001) and pimonidazole scores (p = 0.004). D* (r = 0.508, p = 0.037) and T1 mapping (r = 0.489, p = 0.046) values positively correlated with pimonidazole scores. D* (r = 0.556, p = 0.020) and T1 mapping (r = 0.505, p = 0.039) showed a positive correlation with HIF-1α. The optimal cut-off value of T1 mapping was 941.527, and the sensitivity, specificity, and AUC were 87.5, 77.8, and 0.889 (95% confidence interval [CI]: 0.734–1), respectively.ConclusionIVIM and T1 Mapping are promising methods for non-invasive detection of hypoxia status in chronic liver diseases.
The evolution of cell-free fetal DNA testing: expanded non-invasive prenatal testing and its effect on target populations
Authors: Yang Shaozhe , He Yanqi , Lv Jingshang , Li Rongxiang , Fu Xiuhong 
Published: January 2025

PurposeTo evaluate the clinical performance of expanded non-invasive prenatal testing (NIPT-plus) in screening for fetal chromosome aneuploidy and copy number variations (CNVs) among pregnant women with different risk factors to investigate how the target population of cell-free fetal DNA may change in NIPT-plus.MethodsThe clinical data, test results, confirmatory invasive testing outcomes, and follow-up results of 6,220 pregnant women who underwent NIPT-plus were re-viewed. The performance indicators of the positive predictive value (PPV), positive rate (PR), specificity, and sensitivity in screening for common trisomies, sex chromosomal abnormalities (SCAs), rare autosomal aneuploidies (RAAs), and CNVs were calculated. The PR or PPV of NIPT-plus for screening chromosome aneuploidy and CNVs in women of varying ages, risk factors, and clinical indications were determined.ResultsThe PRs of common trisomies, SCAs, RAAs, and CNVs in NIPT-plus were 0.71, 0.45, 0.32, and 0.59%, respectively, with 100% sensitivity and specificities ranging from 99.69 to 99.87%. The PPVs were 80.95, 30.77, 13.33, and 44.12%, respectively. The high-risk group had higher PRs and PPVs for chromosome aneuploidy, with no significant difference in screening for CNVs. NIPT-plus showed greater PR for aneuploidy in the older age group than in the younger age group, with no significant differences in CNVs screening.ConclusionNIPT-plus was able to effectively screen for chromosome aneuploidy and CNVs. The performance of CNVs screening was not significantly different among different risk factors and age groups. The target population for NIPT-plus should include all pregnant women, not just those at high risk.
Prioritizing breast cancer surgeries: insights from the KRONOS SCORE
Authors: Navarro Lucía , Perez-Bartivas Luis , Ponferrada David , Cejas Javier , Camús Juan Adrián , Rangel Natalia , Porras Maria , Morales Estevez Cristina , Palacios Eito Amalia , Rioja Pilar , Alvarez Marina , Aranda Enrique , De La Haba-Rodríguez Juan 
Published: January 2025

IntroductionIn many healthcare systems, the time to surgery (TTS) is used as a quality measure in breast cancer (BC) care. Although guidelines suggest that a waiting period of up to four weeks is acceptable, this is often exceeded, potentially impacting treatment outcomes. The COVID-19 pandemic highlighted the need to reassess surgical urgency. This study aims to explore the relationship between TTS and clinical outcomes in BC patients, focusing on how TTS influences final tumor stage and lymph node involvement.Materials and methodsA retrospective cohort study was conducted, including 924 women diagnosed with cT1-T3 BC without axillary lymph node involvement between 2014 and 2023. Preoperative staging was done using mammography and ultrasound, while postoperative staging relied on definitive anatomopathological reports. Statistical analyses included chi-square tests, Wilcoxon tests, and binary logistic regression. A prognostic score, the KRONOS SCORE, was developed based on significant variables from the final model, and internal validation was performed.ResultsOut of 924 patients, 781 had ductal carcinomas, 127 had lobular carcinomas, and 16 had other histologies. Breast-conserving surgery was performed on 664 patients, while 260 underwent mastectomy. TTS was less than 8 weeks for 513 patients and more than 8 weeks for 411 patients. No significant differences in tumor size changes were observed based on TTS. However, lymph node involvement increased from 23.8% in patients operated on within 8 weeks to 26.5% in those operated on after 8 weeks, with significant differences noted in poorly differentiated tumors (G3). The KRONOS SCORE, based on age, tumor grade, and size, was validated, showing a higher risk of lymph node involvement with higher scores.DiscussionAlthough TTS did not significantly affect most clinical and pathological parameters, a trend towards increased lymph node involvement with prolonged TTS was observed, particularly in patients with poorly differentiated tumors (G3). The KRONOS SCORE offers a tool for prioritizing surgical patients based on risk factors. However, further multicenter and prospective studies are needed to validate these findings and the KRONOS SCORE model’s effectiveness in different clinical settings.
Tanshinlactone triggers methuosis in breast cancer cells via NRF2 activation
Authors: Lin Wanjun , Huang Zifeng , Zhang Xuening , Zheng Dayuan , Yang Yanchao , Shi Meina , Yang Dongfang , Chu Tong , Ma Wenzhe 
Published: January 2025

BackgroundTanshinlactone is a compound derived from the herb Salvia miltiorrhiza. Breast cancer is the most prevalent malignancy among women globally. While significant strides have been made in breast cancer management, these interventions are often impeded by substantial adverse effects that undermine patients’ quality of life and confront limitations due to the eventual development of multi-drug resistance. Catastrophic macropinocytosis, also called methuosis, as a nonapoptotic cell death associated with cytoplasmic vacuolization, has gained increasing attention, largely because of its potential importance in cancer therapy.MethodsThe effect of tanshinlactone on the growth of human cancer cells was evaluated using sulforhodamine B and colony formation assay. Fluorescent dyes are used to label macropinosomes and lysosomes. Phase contrast, confocal and transmission electron microscopy were employed to observe cell morphological changes. RT-PCR, western blot, lentiviral-mediated gene overexpression, and pharmacological inhibitor assays were comprehensively designed to regulate the identified signaling pathways and confirm the mechanism of tanshinlactone. Human breast cancer cell lines-derived xenograft tumor explants assay was used to evaluate the compound’s efficacy and to assess the induction of methuosis via NRF2 activation by tanshinlactone.ResultsTanshinlactone selectively inhibits the growth of ER+ and HER2+/EGFR + breast cancer cells while showing limited cytotoxicity against other cancer types and normal cells. The selective anti-breast cancer activity is associated with the induction of methuosis, characterized by cytoplasmic vacuolization due to dysfunctional macropinocytosis. This process is mediated by the activation of the transcription factor NRF2, leading to the formation of macropinosomes that fail to fuse with lysosomes or recycle to the plasma membrane, resulting in cell death. The in vitro induction of methuosis via NRF2 activation was replicated in a murine xenograft explants model. Additionally, tanshinlactone demonstrated effectiveness against lapatinib-resistant breast cancer cells, suggesting its potential as a therapeutic agent for overcoming drug resistance in cancer treatment.ConclusionTanshinlactone as a novel therapeutic agent, is capable of selectively inhibiting ER+ and HER2+/EGFR + breast tumors through a unique mechanism of inducing catastrophic macropinocytosis. This regimen holds promise for targeted therapy with minimized side effects and offers a new therapeutic avenue for breast patients with drug-resistant diseases.
Dynamic physiology and transcriptomics revealed the alleviation effect of melatonin on Reaumuria trigyna under continuous alkaline salt stress
Authors: Li Xuebo , Wang Lei , Wang Heyi , Hao Rui , Gao Lunkai , Cui Hongbo , Wu Hai , Wu Xiaodong , Qiao Tong , Bai Weijie , Zhang Liming 
Published: January 2025

IntroductionReaumuria trigyna, a pivotal salt-tolerant plant species in Central Asian salt desert ecosystems, has garnered significant attention due to its resilience under harsh environmental conditions. This study investigates the response mechanisms of melatonin on the dynamic physiology and transcriptomics of Reaumuria trigyna, a critical salt-tolerant plant species in Central Asian salt desert ecosystems. Despite significant progress in understanding plant salt tolerance, research on the positive effects of melatonin on Reaumuria trigyna, particularly its impact on seed germination and the underlying physiological and molecular mechanisms, remains limited.MethodsIn this study, we evaluated the physiological responses of Reaumuria trigyna under continuous alkaline salt stress and examined the effect of melatonin on seed germination.ResultsOur results demonstrate that melatonin at concentrations of 300μmol/L significantly enhances plant growth and promotes the accumulation of osmotic regulators. Notably, melatonin treatment increased the germination rate by 35.48% compared to the alkaline salt stress group, which exhibited a 52.15% lower germination rate than the untreated control. The key mechanism identified involves melatonin’s ability to increase antioxidant enzyme activity, reduce reactive oxygen species and hydrogen peroxide levels, and alter gene expression patterns.DiscussionTranscriptomic analysis revealed significant changes in gene expression, particularly in photosynthetic signal transduction, phytohormone signaling, MAPK signaling, and the peroxisome pathway, which are crucial for the plant’s response to alkaline salt stress. Our findings provide new insights into how melatonin affects plant growth, salt tolerance, seed germination, and gene expression in Reaumuria trigyna under continuous alkaline salt stress. These results address a significant gap in current scientific knowledge and offer valuable theoretical support and practical guidance for cultivating salt-resistant crops and the ecological restoration of salt-affected desert environments.
Selection of suitable reference lncRNAs for gene expression analysis in Osmanthus fragrans under abiotic stresses, hormone treatments, and metal ion treatments
Authors: Zhang Yingting , Yan Qingyu , Xia Hui , Zeng Xiangling , Yang Jie , Cai Xuan , Li Zeqing , Chen Hongguo , Zou Jingjing 
Published: January 2025

IntoductionOsmanthus fragrans, a well-regarded traditional flower in China, holds extensive applications in gardening, food, cosmetics, and traditional Chinese medicine. Despite its importance, research on long non-coding RNAs (lncRNAs) in O. fragrans has been constrained by the absence of reliable reference genes (RGs).MethodsWe employed five distinct algorithms, i.e., delta-Ct, NormFinder, geNorm, BestKeeper, and RefFinder, to evaluate the expression stability of 17 candidate RGs across various experimental conditions.Results and discussionThe results indicated the most stable RG combinations under different conditions as follows: cold stress: lnc00249739 and lnc00042194; drought stress: lnc00042194 and lnc00174850; salt stress: lnc00239991 and lnc00042194; abiotic stress: lnc00239991, lnc00042194, lnc00067193, and lnc00265419; ABA treatment: lnc00239991 and 18S; MeJA treatment: lnc00265419 and lnc00249739; ethephon treatment: lnc00229717 and lnc00044331; hormone treatments: lnc00265419 and lnc00239991; Al3+ treatment: lnc00087780 and lnc00265419; Cu2+ treatment: lnc00067193 and 18S; Fe2+ treatment: lnc00229717 and ACT7; metal ion treatment: lnc00239991 and lnc00067193; flowering stage: lnc00229717 and RAN1; different tissues: lnc00239991, lnc00042194, lnc00067193, TUA5, UBQ4, and RAN1; and across all samples: lnc00239991, lnc00042194, lnc00265419 and UBQ4. The reliability of these selected RGs was further validated by analyzing the expression patterns of lnc00003036, lnc00126603, and lnc00250780. This study represents the first comprehensive evaluation of lncRNA RG stability in O. fragrans, significantly enhancing the accuracy and depth of lncRNA expression analyses in this species, contributing to advancements in plant stress resistance breeding and improving environmental adaptability.