bluuebunny/crossref_metadata_2025_split huggingface dataset:
A systematic review and meta‐analysis of the prevalence and correlation of mild cognitive impairment in sarcopenia
Authors: Yang Ying , Xiao Mengmeng , Leng Lin , Jiang Shixie , Feng Lei , Pan Gaofeng , Li Zheng , Wang Yan , Wang Jiang , Wen Yanting , Wu Dan , Yang Yongxue , Huang Pan 
Published: December 2022

AbstractSarcopenia is a progressive skeletal muscle disorder involving the loss of muscle mass and function, associated with an increased risk of disability and frailty. Though its prevalence in dementia has been studied, its occurrence in mild cognitive impairment (MCI) has not been well established. As MCI is often a prelude to dementia, our study aims to investigate the prevalence of MCI among individuals with sarcopenia and to also ascertain whether sarcopenia is independently associated with MCI. The Cochrane Library, PubMed, Ovid, Embase and Web of Science were systematically searched for articles on MCI and/or sarcopenia published from inception to 1 February 2022. We reviewed the available literature on the number of individuals with MCI and/or sarcopenia and calculated odds ratios (ORs) of sarcopenia in MCI and MCI in sarcopenia, respectively. Statistical analyses were performed using the meta package in Stata, Version 12.0. A total of 13 studies and 27 428 patients were included in our analysis. The pooled prevalence of MCI in participants with sarcopenia was 20.5% (95% confidence interval [CI]: 0.140–0.269) in a total sample of 2923 cases with a high level of heterogeneity (P < 0.001; I2 = 95.4%). The overall prevalence of sarcopenia with MCI was 9.1% (95% CI: 0.047–0.134, P < 0.001; I2 = 93.0%). For overall ORs, there were 23 364 subjects with a mean age of 73 years; the overall adjusted OR between MCI and sarcopenia was 1.46 (95% CI: 1.31–1.62). Slight heterogeneity in both adjusted ORs (P = 0.46; I2 = 0%) was noted across the studies. The prevalence of MCI is relatively high in patients with sarcopenia, and sarcopenia may be a risk factor for MCI.
Application of dual‐source computed tomography in the diagnosis of thyroid cancer and evaluation of biological behaviors
Authors: Wu Wenhui , Fang Xuewen , Li Jianming , Zhang An , Zou Yujian , Zheng Xiaolin 
Published: December 2022

AbstractObjectiveThyroid cancer (TC) is an extremely prevailing malignant endocrine tumor. Therefore, effective diagnostic tools are necessary. This study explored the application value of dual‐source computed tomography (DSCT) in TC diagnosis and biological behavior assessment.MethodsThis study retrospectively selected 68 TC patients and another 74 benign patients with thyroid adenoma, nodular goiter, or adenomatous hyperplasia. All patients were confirmed by pathological examination and underwent DSCT examination. The iodine concentration (IC) obtained from plain computed tomography (CT) scanning and normalized iodine concentration (NIC) in the arterial phase and venous phase were recorded. The positive expression rates of estrogen receptor alpha (ERα), estrogen receptors beta (ERβ), and Ki67 in pathological tissues were determined by immunohistochemistry, and their correlation with IC in plain CT was assessed by Pearson correlation analysis, respectively. The diagnostic values of IC in plain CT and venous phase NIC in TC patients were evaluated using the receiver operating characteristic curve.ResultsMalignant patients had lower IC in plain DSCT scanning, venous phase NIC, and ERβ, and higher ERα and Ki67 than benign patients. IC level in plain DSCT scanning was inversely‐correlated with ERα and Ki‐67 positive expression rates, but positively‐related to ERβ to different degrees. For the diagnosis of TC patients, the AUC of IC level in plain DSCT was 0.771, with a cut‐off value of 1.250 (97.06% sensitivity and 41.89% specificity), and the AUC of venous phase NIC was 0.738, with a cut‐off value of 0.825 (100% sensitivity and 43.24% specificity).ConclusionThe IC level obtained from DSCT scanning could assist in the differential diagnosis of malignant and benign thyroid nodules and evaluation of biological behaviors.
Bile proteome analysis by high‐precision mass spectrometry to examine novel biomarkers of primary sclerosing cholangitis
Authors: Kan Motoyasu , Chiba Tetsuhiro , Konno Ryo , Kouchi Yusuke , Mishima Takashi , Kawashima Yusuke , Kishimoto Takashi , Ohtsuka Masayuki , Ohara Osamu , Kato Naoya 
Published: December 2022

AbstractBackgroundPrimary sclerosing cholangitis (PSC) is a chronic inflammatory disease of unknown etiology that affects the intra‐ and extrahepatic bile ducts. The present study examined the utility of a bile proteome analysis using a high‐sensitivity mass spectrometer to comprehensively screen for novel PSC biomarkers.MethodsBile endoscopically collected from patients with PSC, common bile duct stones, and biliary tract cancer were subjected to high‐precision liquid chromatography/mass spectrometry. Some of the proteins specifically up‐regulated in the bile of the PSC group were re‐examined by an enzyme‐linked immunosorbent assay.ResultsA total of 8094 proteins were successfully identified and 332 were specifically up‐regulated in the PSC group. The bioinformatics analysis showed that proteins involved in the proliferation and activation of diverse inflammatory cells were up‐regulated in the PSC group. A receiver operating characteristic curve analysis showed good area under the curve values for interleukin‐8 and annexin A1 (ANXA1) (0.836 and 0.914, respectively). Immunostaining for ANXA1 revealed its strong expression in inflammatory cells infiltrating the peripheral biliary tract in PSC livers.ConclusionA bile proteome analysis is a useful tool for elucidating the pathogenesis of PSC and developing new diagnostic approaches. Therefore, ANXA1 has potential as a bile biomarker for PSC.
Synthesis and radiolabeling of a polar [125I]I‐1,2,4,5‐tetrazine
Authors: Bidesi Natasha , Shalgunov Vladimir , Battisti Umberto Maria , Hvass Lars , Jørgensen Jesper Tranekjær , Poulie Christian B. M. , Jensen Andreas I. , Kjaer Andreas , Herth Matthias M. 
Published: December 2022

Pretargeting imaging has gained a lot of prominence, due to its excellent bioorthogonality and improved imaging contrast compared to conventional imaging. A new iodo tetrazine (Tz) derivative has been synthesized and further developed into the corresponding iodine‐125 (125I) analog (12), via the trimethylstannane precursor. Radiolabeling with either N‐chlorosuccinimide or chloramine‐T, in either MeCN or MeOH proceeded with a radiochemical conversion (RCC) of >80%. Subsequent deprotection only proved successful, among the tested conditions, when the radiolabeled Tz was stirred in 6‐M HCl(aq.) at 60°C for 2.5 h. To the best of our knowledge, this is the first H‐tetrazine labeled with iodine. In vivo investigations on the pretargeting ability of 12 are currently under way.
Emergence of a unique SARS‐CoV‐2 Delta sub‐cluster harboring a constellation of co‐appearing non‐Spike mutations
Authors: Banerjee Anindita , Mazumder Anup , Roy Jayita , Das Jagyashila , Majumdar Agniva , Chatterjee Ananya , Biswas Nidhan K. , Chawla Sarkar Mamta , Das Saumitra , Dutta Shanta , Maitra Arindam 
Published: December 2022

AbstractAccumulation of diverse mutations across the structural and nonstructural genes is leading to rapid evolution of SARS‐CoV‐2, altering its pathogenicity. We performed whole genome sequencing of 239 SARS‐CoV‐2 RNA samples collected from both adult and pediatric patients across eastern India (West Bengal), during the second pandemic wave in India (April–May 2021). In addition to several common spike mutations within the Delta variant, a unique constellation of eight co‐appearing non‐Spike mutations was identified, which revealed a high degree of positive mutual correlation. Our results also demonstrated the dynamics of SARS‐CoV‐2 variants among unvaccinated pediatric patients. 41.4% of our studied Delta strains harbored this signature set of eight co‐appearing non‐Spike mutations and phylogenetically out‐clustered other Delta sub‐lineages like 21J, 21A, or 21I. This is the first report from eastern India that portrayed a landscape of co‐appearing mutations in the non‐Spike proteins, which might have led to the evolution of a distinct Delta subcluster. Accumulation of such mutations in SARS‐CoV‐2 may lead to the emergence of “vaccine‐evading variants.” Hence, monitoring of such non‐Spike mutations will be significant in the formulation of any future vaccines against those SARS‐CoV‐2 variants that might evade the current vaccine‐induced immunity, among both the pediatric and adult populations.
Impact of high‐risk Human Papillomavirus genotyping in cervical disease in the Northern region of Portugal: Real‐world data from regional cervical cancer screening program
Authors: Rosário Andreia , Sousa Ana , Marinho‐Dias Joana , Medeiros Rui , Lobo Cláudia , Leça Luís , Coimbra Nuno , Tavares Fernando , Baldaque Inês , Martins Gabriela , Monteiro Paula , Henrique Rui , Sousa Hugo 
Published: December 2022

AbstractCervical cancer prevention is based on primary prevention with vaccines against Human Papillomavirus (HPV) and secondary prevention by screening with High‐Risk‐HPV (Hr‐HPV) detection. Since 2017, cervical cancer screening in women aged 25−60 years has been performed in Portugal using Hr‐HPV detection, followed by cytology in Hr‐HPV‐positive cases. Herein we report the prevalence of Hr‐HPV genotypes and cytological abnormalities among 462 401 women (mean age: 43.73 ± 10.79; median age: 45; range: 24−66 years) that participated in the Regional Cervical Cancer Screening Program of the Northern Region of Portugal, performed between August 2016 and December 2021. Overall, we describe a prevalence rate of 12.50% for Hr‐HPV varying from 20.76% at age 25% to 8.32% at age 64. The five most common Hr‐HPV genotypes identified were HPV‐68 (16.09%), HPV‐31 (15.30%), HPV‐51 (12.96%), HPV‐16 (11.06%), and HPV‐39 (11.01%). The prevalence of Hr‐HPV included in the nonavalent vaccine (HPV‐9valent) was 55.00% ranging from 47.78% to 59.18% across different age groups. Considering positive Hr‐HPV cases, 65.68% had a Negative for Intraepithelial Lesion or Malignancy (NILM) cytology, 20.83% atypical squamous cells of undetermined significance (ASC‐US), 8.85% Low‐Grade Squamous Intraepithelial Lesion (LSIL), 1.65% High‐Grade Squamous Intraepithelial Lesion (HSIL), 2.85% ASC‐H, 0.09% Atypical Glandular Cells, 0.02% Adenocarcinomas, and 0.02% Squamous Cell Carcinoma (SCC). Our analysis revealed that HPV‐9val genotypes were responsible for 52.13% NILM, 59.21% ASC‐US, 55.06% LSIL, 90.14% HSIL, 83.50% ASC‐H, and 100.00% SCC. Furthermore, multiple Hr‐HPV infections (risk ratio [RR] = 1.46; 95% confidence interval [CI] 1.34−1.58), HPV‐16/18 (RR = 5.16; 95% CI 4.75−5.93), or HPV‐9val genotypes (RR = 5.23; 95% CI 4.68−5.85) were associated with a significant risk of developing >  HSIL (p < 0.001). To date, this is the largest study on Hr‐HPV genotyping in cervical cancer screening that includes data from a complete cycle of the screening program. Our findings suggest a high prevalence of HPV‐9valent genotypes and a significant association with an increased risk of developing > HSIL. This constitutes important data for health authorities, which may help define the future of vaccination and cervical cancer screening strategies.
Gold nanoparticle conjugate‐based lateral flow immunoassay (LFIA) for rapid detection of RBD antigen of SARS‐CoV‐2 in clinical samples using a smartphone‐based application
Authors: Prakashan Drishya , Shrikrishna Narlawar S. , Byakodi Manisha , Nagamani K. , Gandhi Sonu 
Published: December 2022

AbstractThe coronavirus disease 2019 (COVID‐19) pandemic has emphasized the need for development of a rapid diagnostic device for the effective treatment and its mitigation. Lateral flow immunoassay (LFIA) belongs to a class of diagnostic devices, which has the benefit of providing quick results, easy to handle, low cost, and on‐site applicable. So far, several LFIA has been developed for the detection of infectious severe acute respiratory syndrome‐coronavirus‐2 (SARS‐CoV‐2), however, only a few of them are antigen (Ag)‐based. Here, we describe an antibody (Ab)‐labeled gold nanoparticles (AuNPs)‐based LFIA (AuNPs‐LFIA) for the detection of Receptor‐Binding Domain (RBD) of SARS‐CoV‐2. For this, RBD Ab of SARS‐CoV‐2 was conjugated with the AuNPs, which served as a detecting probe. The fabricated LFIA strip was optimized for different parameters such as membrane pore size, blocking conditions, Ab coating concentration, and conjugate incubation. The optimized LFIA strips were validated in spiked buffer samples and the optimal limit of detection was found to be 1 ng/ml, which was confirmed by a smartphone‐based application. Moreover, the developed AuNPs‐LFIA strips effectively detected RBD Ag in 100 clinical samples with 94.3% sensitivity and 90.9% specificity in clinical samples when compared with the gold standard (RT‐PCR). The fabricated LFIAs are reported to have storage stability of up to 21 days at 4°C and room temperature (RT). Hence, the developed LFIA can be used as a portable, cost‐effective diagnostic device for rapid detection of SARS‐CoV‐2.
Influence of Lactobacillus reuteri, Bifidobacterium animalis subsp. lactis, and prebiotic inulin on dysbiotic dental biofilm composition ex vivo
Authors: Colamarino Aaron N. , Johnson Thomas M. , Boudreaux Daniel M. , Dutner Joseph M. , Stancoven Brian W. , Lincicum Adam R. , Akers Joshua A. 
Published: December 2022

AbstractBackgroundProbiotic bacterial supplementation has shown promising results in the treatment of periodontitis and the maintenance of periodontal health. The purpose of this investigation was to evaluate the influence of Lactobacillus reuteri or Bifidobacterium animalis subsp. lactis supplementation with and without prebiotic inulin on biofilm composition using an ex vivo biofilm model.MethodsSubgingival plaque specimens from three periodontitis‐affected human donors were used to grow biofilms on hydroxyapatite disks in media supplemented with varying combinations of prebiotic inulin, Lactobacillus reuteri, and Bifidobacterium animalis subsp. lactis. Relative abundances of bacterial genera present in mature biofilms were evaluated using 16S rRNA next‐generation sequencing. Diversity metrics of microbial communities were evaluated using a next‐generation microbiome bioinformatics platform.ResultsInulin supplementation produced statistically significant dose‐dependent increases in relative abundances of Lactobacillus and Bifidobacterium species (p < 0.001) with concomitant decreases in relative abundances of Streptococcus, Veillonella, Fusobacterium, Parvimonas, and Prevotella species (p < 0.001). Inoculation with L. reuteri or B. animalis subsp. lactis increased the relative abundance of only the supplemented probiotic genera (p < 0.05). Supplemental inulin led to a statistically significant decrease in biofilm alpha diversity (p < 0.001).ConclusionsThe described ex vivo model appears suitable for investigating the effects of probiotic bacteria, prebiotic oligosaccharides, and combinations thereof on biofilm composition and complexity. Within the limitations imposed by this model, results from the present study underscore the potential for prebiotic inulin to modify biofilm composition favorably. Additional research further elucidating biologic rationale and controlled clinical research defining therapeutic benefits is warranted.
Graphical tools for visualizing the results of network meta‐analysis of multicomponent interventions
Authors: Seitidis Georgios , Tsokani Sofia , Christogiannis Christos , Kontouli Katerina‐Maria , Fyraridis Alexandros , Nikolakopoulos Stavros , Veroniki Areti Angeliki , Mavridis Dimitris 
Published: December 2022

AbstractNetwork meta‐analysis (NMA) is an established method for assessing the comparative efficacy and safety of competing interventions. It is often the case that we deal with interventions that consist of multiple, possibly interacting, components. Examples of interventions' components include characteristics of the intervention, mode (face‐to‐face, remotely etc.), location (hospital, home etc.), provider (physician, nurse etc.), time of communication (synchronous, asynchronous etc.) and other context related components. Networks of multicomponent interventions are typically sparse and classical NMA inference is not straightforward and prone to confounding. Ideally, we would like to disentangle the effect of each component to find out what works (or does not work). To this aim, we propose novel ways of visualizing the NMA results, describe their use, and illustrate their application in real‐life examples. We developed an R packageviscompto produce all the suggested figures.
Inhibitory effect of pre‐gelatinized dialdehyde starch on heat‐induced deterioration of sea cucumber
Authors: Cao Honghua , Liu Yonghao , Zhi Ling , Jing Shutong , Li Zhengshengao , Wang Dongfeng , Xu Ying , Sun Xun 
Published: December 2022

AbstractBACKGROUNDThis study sought to investigate the inhibitory effect of pre‐gelatinized dialdehyde starch (P‐DAS) on the deterioration of sea cucumber during high‐temperature sterilization.RESULTSIt was found that pre‐gelatinization reduced crystallinity and average molecular weight of dialdehyde starch (DAS), exposed free aldehyde groups, improved the solubility, and unified the particle sizes. According to the texture profiles of sea cucumber, the crosslinking power of P‐DAS was higher than that of DAS. The results of free amino content, total soluble substance, water retention, water distribution, relaxation time and scanning electron microscopy all showed that the crosslinking effect was dose‐dependent on crosslinking agent.CONCLUSIONThese results have proved that large molecules such as P‐DAS, when properly handled, could also efficiently enter collagen hydrogels and perform crosslinking, providing reference for the development of new protein food stabilizing agents. © 2022 Society of Chemical Industry.
Effects of steam explosion on the nutritional and functional properties of black‐grained wheat bran and its application
Authors: Liu Yuxiu , Huang Shuhua , Meng Tianqi , Wang Yizhao , Zhang Zhengmao 
Published: December 2022

AbstractBackgroundIn recent years, an increasing interest in healthy functional foods has been documented among health‐conscious consumers. Steam explosion (SE)‐treated black‐grained wheat (BGW) bran was explored for the development of chiffon cakes with high nutritional and functional value.ResultsThe content of crude fat and total starch decreased with increasing SE pressure, whereas water‐holding capacity and antioxidant activity increased, suggesting SE at 0.6–1.0 MPa could be an effective technique for enhancing the nutritional and functional properties of wheat bran. The protein, iron, zinc, manganese, selenium, and soluble dietary fiber contents, the water‐holding, oil‐binding, swelling, cholesterol binding, and cation‐exchange capacities, and antioxidant activity of SE BGW bran were better than those of SE white‐grained wheat bran. The addition of SE bran (0.8 MPa) to flour significantly decreased the peak viscosity, final viscosity, and setback and increased the pasting temperature. The effect of SE bran on the pasting properties of low‐gluten and medium‐gluten flour was stronger than that of high‐gluten flour. SE BGW bran altered the physicochemical properties of chiffon cakes. When 6% SE BGW bran (0.8 MPa) was added, chiffon cakes exhibited good specific volume, hardness, chewiness, and other sensory qualities.ConclusionsThese results indicate that SE at 0.6–1.0 MPa is an effective technique for enhancing the nutritional and functional properties of wheat bran. SE BGW bran can be alternatives to food materials for developing health functional cereal‐based products. © 2022 Society of Chemical Industry.
Defining a textbook outcome for the resection of colorectal liver metastases
Authors: Neary Colm , O'Brien Lukas , McCormack Emilie , Kelly Michael , Bolger Jarlath , McEntee Gerard , Conneely John 
Published: December 2022

AbstractIntroductionTextbook outcomes (TBO) are composite measures of care which may be superior in assessing quality compared to traditional methods. We aim to define TBO which are specific to surgical resection of colorectal liver metastases, and investigate their impact on survival.MethodsSingle center analysis of all liver resections performed at our center from 2009 to 2020. A Cox model was used to identify perioperative outcomes which impacted on overall survival. These were retained with important postoperative outcomes to form a “TBO.” The impact of a TBO on overall survival was investigated using Kaplan−Meier curve analysis.ResultsTBO was achieved in 72.2% (197/273) of resections. Major morbidity (Clavien−Dindo ≥3) at 19.4% was the major limiting factor in not achieving a TBO. TBO was associated with improved 3‐year (77% vs. 55%), 5‐year (60.7% vs. 42.5%), and median (93 vs. 44 months) overall survival (log‐rank test, p = 0.006). Multivariable analysis revealed age >65 years, American Society of Anaesthesiologists Grade III–IV, and resection of >2 segments as factors predictive of not achieving a TBO.ConclusionTBO is a useful composite measure in surgery for colorectal liver metastases. It can highlight areas which may be targeted for quality improvement and be useful as a tool to examine variation between centers. Achieving a TBO is associated with a significant improvement in survival.
Preclinical in vivo animal models of intervertebral disc degeneration. Part 1: A systematic review
Authors: Poletto Daniel L. , Crowley James D. , Tanglay Onur , Walsh William R. , Pelletier Matthew H. 
Published: December 2022

AbstractIntervertebral disc degeneration (IVDD), a widely recognized cause of lower back pain, is the leading cause of disability worldwide. A myriad of preclinical in vivo animal models of IVDD have been described in the literature. There is a need for critical evaluation of these models to better inform researchers and clinicians to optimize study design and ultimately, enhance experimental outcomes. The purpose of this study was to conduct an extensive systematic literature review to report the variability of animal species, IVDD induction method, and experimental timepoints and endpoints used in in vivo IVDD preclinical research. A systematic literature review of peer‐reviewed manuscripts featured on PubMed and EMBASE databases was conducted in accordance with PRISMA guidelines. Studies were included if they reported an in vivo animal model of IVDD and included details of the species used, how disc degeneration was induced, and the experimental endpoints used for analysis. Two‐hundred and fifty‐nine (259) studies were reviewed. The most common species, IVDD induction method and experimental endpoint used was rodents(140/259, 54.05%), surgery (168/259, 64.86%) and histology (217/259, 83.78%), respectively. Experimental timepoint varied greatly between studies, ranging from 1 week (dog and rodent models), to >104 weeks in dog, horse, monkey, rabbit, and sheep models. The two most common timepoints used across all species were 4 weeks (49 manuscripts) and 12 weeks (44 manuscripts). A comprehensive discussion of the species, methods of IVDD induction and experimental endpoints is presented. There was great variability across all categories: animal species, method of IVDD induction, timepoints and experimental endpoints. While no animal model can replicate the human scenario, the most appropriate model should be selected in line with the study objectives to optimize experimental design, outcomes and improve comparisons between studies.