bluuebunny/crossref_metadata_2025_split huggingface dataset:
Multiwavelength study of NGC 1365: The obscured active nucleus and off-nuclear compact X-ray sources
Authors: Swain Subhashree , Dewangan Gulab Chand , Shalima P , Tripathi Prakash , Latha K V P 
Published: February 2023

ABSTRACTWe present a multiwavelength study of the active nucleus and the off-nuclear X-ray sources in the nearby spiral galaxy, NGC 1365 using three simultaneous UV/X-ray observations by AstroSat over a two months period and archival IR observations performed with Spitzer and Herschel. Utilizing the data from the Soft X-ray Telescope (SXT) onboard AstroSat, we find spectral variability mainly caused by the variation in the X-ray column density, (NH ∼ 1022–1023 cm−2). With the accurate spatial resolution of the UVIT onboard AstroSat, we separate the intrinsic AGN flux from the host galaxy emission and then correct for the Galactic and the internal reddening. We detect no significant variation in the NUV emission over the observation period. The AGN in FUV band is undetectable due to heavy intrinsic extinction. Further, the multiwavelength IR/UV/X-ray AGN SED reveals that the AGN is in a low-luminosity phase with accretion rate ∼0.01 LEdd. The steady UV emission and strong X-ray absorption variability suggest that the obscuring clouds are likely compact and affect the compact X-ray source only and do not possibly cover the extended UV emitting region. In addition, the UVIT is able to resolve two bright spots at a radius of 7 arcsec (∼6.3 Kpc) from the central nucleus in the South-West (SW) direction. In the UVIT image of the entire galaxy, we identify UV counterparts to four Chandra identified bright X-ray sources. One well-known ultra-luminous X-ray source (ULX) NGC 1365 X2 is identified with its UV counterpart at 86 arcsec from the nucleus in the North-East (NE) direction from the active nucleus.
Nelsonia goldmani(Rodentia: Cricetidae)
Authors: León-Tapia M Ángel 
Published: February 2023

AbstractNelsonia goldmani Merriam, 1903 is a cricetid rodent commonly called Goldman’s diminutive woodrat. It is a woodrat of medium size with grayish dorsal pelage; the characteristic long tail is covered with long hairs and ends with a black tip. Nelsonia goldmani is one of two species in the genus Nelsonia. It is endemic to the central-west highlands of Mexico, recorded in a few localities of the Trans-Mexican Volcanic Belt at elevations from 2,000 to 3,100 m. It inhabits areas near rocky ravines, cliffs, and hillsides with coniferous, pine–oak, and cloud forests. Nelsonia goldmani is listed as “Endangered” (EN) by the International Union for Conservation of Nature and Natural Resources due to its fragmented distribution and the decrease in the quality and extent of its habitat.
MicroRNA-145 and microRNA-486 are potential serum biomarkers for vascular calcification
Authors: Fernández-Villabrille Sara , Martín-Carro Beatriz , Martín-Vírgala Julia , Alonso-Montes Cristina , Palomo-Antequera Carmen , García-Castro Raúl , López-Ongil Susana , Dusso Adriana S , Fernández-Martín José Luis , Naves-Díaz Manuel , Cannata-Andía Jorge B , Carrillo-López Natalia , Panizo Sara 
Published: February 2023

ABSTRACT

Introduction
MicroRNAs (miRs) regulate vascular calcification (VC), and their quantification may contribute to suspicion of the presence of VC.

Methods
The study was performed in four phases. Phase 1: miRs sequencing of rat calcified and non-calcified aortas. Phase 2: miRs with the highest rate of change, plus miR-145 [the most abundant miR in vascular smooth muscle cells (VSMCs)], were validated in aortas and serum from rats with and without VC. Phase 3: the selected miRs were analyzed in epigastric arteries from kidney donors and recipients, and serum samples from general population. Phase 4: VSMCs were exposed to different phosphorus concentrations, and miR-145 and miR-486 were overexpressed to investigate their role in VC.

Results
miR-145, miR-122-5p, miR-486 and miR-598-3p decreased in the rat calcified aortas, but only miR-145 and miR-486 were detected in serum. In human epigastric arteries, miR-145 and miR-486 were lower in kidney transplant recipients compared with donors. Both miRs inversely correlated with arterial calcium content and with VC (Kauppila index). In the general population, the severe VC was associated with the lowest serum levels of both miRs. The receiver operating characteristic curve showed that serum miR-145 was a good biomarker of VC. In VSMCs exposed to high phosphorus, calcium content, osteogenic markers (Runx2 and Osterix) increased, and the contractile marker (α-actin), miR-145 and miR-486 decreased. Overexpression of miR-145, and to a lesser extent miR-486, prevented the increase in calcium content induced by high phosphorus, the osteogenic differentiation and the loss of the contractile phenotype.

Conclusion
miR-145 and miR-486 regulate the osteogenic differentiation of VSMCs, and their quantification in serum could serve as a marker of VC.
Nutritional markers accompanying acquired chylothorax in infants: a systematic review
Authors: Marzotto Kevin N , Choudhary Tuhin , Wright Laura A , Howell Meghan P , Kimball Thomas R , Pigula Frank A , Piggott Kurt D 
Published: February 2023

Abstract

Context
Chylothorax is a well-established acquired complication of thoracic surgery in infants. Current data suggest acquired chylothorax may affect infant growth and nutrition because of a loss of essential nutrients via chylous effusion.

Objective
The 3 objectives for this study were: (1) identify nutritional markers affected by the development of acquired chylothorax in infants; (2) highlight the variability in methods used to assess nutritional status and growth in this patient population; and (3) highlight nutritional deficits that can serve as treatment targets during postoperative feeding protocols.

Data Sources
A systematic literature search was conducted between May 31, 2021, and June 21, 2022, using the PubMed, Embase, CINAHL, and Web of Science databases. Search terms included, but were not limited to, “chylothorax,” “infants,” and “nutrition.”

Data Extraction
Inclusion criteria required studies that measured quantitative markers of nutrition in ≥10 participants aged &lt;1 year with acquired chylothorax. A total of 575 studies were screened and all but 4 were eliminated. Nutritional markers were categorized into 4 different groups: total serum protein level, triglyceride levels, growth velocity, and weight for length.

Data Analysis
The variation in methods, time points, interventional groups, and nutritional markers did not facilitate a meta-analysis. Risk of bias was assessed using the Cochrane Risk of Bias in Nonrandomized Studies assessment tool.

Conclusion
This review highlights the need for reliable quantitative markers of nutrition that will enable providers to assess the nutritional needs of infants with chylothorax. Future studies must focus on measuring markers of nutrition at regular intervals in larger study populations.
Non-governmental organizations’ motivation to diversify: self-interest or operation-related? Evidence from Uganda
Authors: Dang Canh Thien , Owens Trudy 
Published: February 2023

Abstract
Understanding the mechanisms that guide non-governmental organizations’ (NGOs) managerial decisions is a key to effective development policies. One fundamentally strategic decision is the number of activities an NGO offers. We provide a conceptual framework based on the agency theory to study the motivations underlying strategic decisions of development NGOs in Uganda. We test whether diversifying into many activities is driven by operational reasons or by personal gains of NGO managers. Following a historic flood in 2007, NGOs that rely more on contractual income offer fewer activities than their counterparts in less affected areas. The results support theoretical explanations that operational motives such as risk-reduction or cost complementarity dominate personal and for-profit-like motives. Our article contributes to the debates around the ethical and governmental foundation of the non-profit sector, highlighting the different roles of personal and operational aspects in the decision-making process.
Introduction to Faraday tomography and its future prospects
Authors: Takahashi Keitaro 
Published: February 2023

AbstractFaraday tomography is a new method of the study of cosmic magnetic fields enabled by broad-band low-frequency radio observations. Using Faraday tomography it is possible to obtain the Faraday dispersion function, which contains information on the line-of-sight distributions of magnetic fields, thermal electron density, and cosmic ray electron density by measuring the polarization spectrum from a source of synchrotron radiation over a wide band. Furthermore, by combining it with two-dimensional imaging, Faraday tomography allows us to explore the three-dimensional structure of polarization sources. Faraday tomography has been active over the last 20 years, since the broad-band observation has become technically feasible, and polarization sources such as interstellar space, supernova remnants, and galaxies have been investigated. However, the Faraday dispersion function is mathematically the Fourier transform of the polarization spectrum. And since the observable band is finite, it is impossible to obtain a complete Faraday dispersion function by performing a Fourier transform. For this purpose, various methods have been developed to accurately estimate the Faraday dispersion function from the observed polarization spectrum. In addition, the Faraday dispersion function does not directly reflect the distribution of magnetic field, thermal electron density, and cosmic ray electron density in the physical space, and its physical interpretation is not straightforward. Despite these two difficult problems, Faraday tomography is attracting much attention because it has great potential as a new method for studying cosmic magnetic fields and magnetized plasmas. In particular, the next-generation radio telescope SKA (Square Kilometre Array) is capable of polarization observation with unprecedented sensitivity and broad bands, and the application of Faraday tomography is expected to make dramatic progress in the field of cosmic magnetic fields. In this review, we explain the basics of Faraday tomography with simple and instructive examples. Representative algorithms to realize Faraday tomography are introduced, and some applications are shown.
Sucrose-induced auxin conjugate hydrolase restores symbiosis in aMedicagocytokinin perception mutant
Authors: Molla Firoz , Kundu Anindya , DasGupta Maitrayee 
Published: February 2023

AbstractRhizobia–legume interactions recruit cytokinin for the induction of nodule primordia in the cortex. Cytokinin signaling regulates auxin transport and biosynthesis, causing local auxin accumulation, which triggers cortical cell division. Since sugar signaling can trigger auxin responses, we explored whether sugar treatments could rescue symbiosis in the Medicago truncatula cytokinin response 1 (cre1) mutant. Herein, we demonstrate that sucrose and its nonmetabolizable isomer turanose can trigger auxin response and recover functional symbiosis in cre1, indicating sucrose signaling to be necessary for the restoration of symbiosis. In both M. truncatula A17 (wild type) and cre1, sucrose signaling significantly upregulated IAA-Ala Resistant 3 (IAR33), encoding an auxin conjugate hydrolase, in rhizobia-infected as well as in uninfected roots. Knockdown of IAR33 (IAR33-KD) significantly reduced nodulation in A17, highlighting the importance of deconjugation-mediated auxin accumulation during nodule inception. In cre1, IAR33-KD restricted the sucrose-mediated restoration of functional symbiosis, suggesting that deconjugation-mediated auxin accumulation plays a key role in the absence of CRE1-mediated auxin biosynthesis and transport control. Overexpression of IAR33 also restored functional symbiosis in cre1, further suggesting that IAR33 mediates auxin accumulation in response to sucrose signaling. Since all the observed sucrose-mediated responses were common to A17 and cre1, deconjugation-mediated auxin response appeared to be independent of CRE1, which normally governs local auxin accumulation in the presence of rhizobia. We propose that sucrose-dependent restoration of symbiosis in cre1 occurs by the activation of IAR33-mediated auxin deconjugation.
Manganese concentration affects chloroplast structure and the photosynthetic apparatus in Marchantia polymorpha
Authors: Messant Marine , Hani Umama , Hennebelle Thaïs , Guérard Florence , Gakière Bertrand , Gall Andrew , Thomine Sébastien , Krieger-Liszkay Anja 
Published: February 2023

Abstract
Manganese (Mn) is an essential metal for plant growth. The most important Mn-containing enzyme is the Mn4CaO5 cluster that catalyzes water oxidation in photosystem II (PSII). Mn deficiency primarily affects photosynthesis, whereas Mn excess is generally toxic. Here, we studied Mn excess and deficiency in the liverwort Marchantia polymorpha, an emerging model ideally suited for analysis of metal stress since it accumulates rapidly toxic substances due to the absence of well-developed vascular and radicular systems and a reduced cuticle. We established growth conditions for Mn excess and deficiency and analyzed the metal content in thalli and isolated chloroplasts. In vivo super-resolution fluorescence microscopy and transmission electron microscopy revealed changes in the organization of the thylakoid membrane under Mn excess and deficiency. Both Mn excess and Mn deficiency increased the stacking of the thylakoid membrane. We investigated photosynthetic performance by measuring chlorophyll fluorescence at room temperature and 77 K, measuring P700 absorbance, and studying the susceptibility of thalli to photoinhibition. Nonoptimal Mn concentrations changed the ratio of PSI to PSII. Upon Mn deficiency, higher non-photochemical quenching was observed, electron donation to PSI was favored, and PSII was less susceptible to photoinhibition. Mn deficiency seemed to favor cyclic electron flow around PSI, thereby protecting PSII in high light. The results presented here suggest an important role of Mn in the organization of the thylakoid membrane and photosynthetic electron transport.
Tetratricopeptide-containing SMALL KERNEL 11 is essential for the assembly of cytochrome c oxidase in maize mitochondria
Authors: Ren Zhenjing , Fan Kaijian , Zhen Sihan , Zhang Jie , Liu Yan , Fu Junjie , Qi Chunlai , Wei Qianhan , Du Yao , Tatar Wurinile , Zhang Xiaofeng , Wang Guoying , Rasmusson Allan G , Wang Jianhua , Liu Yunjun 
Published: February 2023

Abstract
Assembly of the functional complexes of the mitochondrial respiratory chain requires sophisticated and efficient regulatory mechanisms. In plants, the subunit composition and assembly factors involved in the biogenesis of cytochrome c oxidase (complex IV) are substantially less defined than in mammals and yeast. In this study, we cloned maize (Zea mays) Small kernel 11 (Smk11) via map-based cloning. Smk11 encodes a mitochondria-localized tetratricopeptide repeat protein. Disruption of Smk11 severely affected the assembly and activity of mitochondrial complex IV, leading to delayed plant growth and seed development. Protein interactions studies revealed that SMK11 might interact with four putative complex IV assembly factors, Inner membrane peptidase 1A (ZmIMP1A), MYB domain protein 3R3 (ZmMYB3R-3), cytochrome c oxidase 23 (ZmCOX23), and mitochondrial ferredoxin 1 (ZmMFDX1), among which ZmMFDX1 might interact with subunits ZmCOX6a and ZmCOX-X1; ZmMYB3R-3 might also interact with ZmCOX6a. The mutation of SMK11 perturbed the normal assembly of these subunits, leading to the inactivation of complex IV. The results of this study revealed that SMK11 serves as an accessory assembly factor required for the normal assembly of subunits into complex IV, which will accelerate the elucidation of the assembly of complex IV in plant mitochondria.
Estimating the Between-Issue Variation in Party Elite Cue Effects
Authors: Tappin Ben M 
Published: February 2023

AbstractParty elite cues are among the most well-established influences on citizens’ political opinions. Yet, there is substantial variation in effect sizes across studies, constraining the generalizability and theoretical development of party elite cues research. Understanding the causes of variation in party elite cue effects is thus a priority for advancing the field. In this paper, I estimate the variation in party elite cue effects that is caused simply by heterogeneity in the policy issues being examined, through a reanalysis of data from existing research combined with an original survey experiment comprising 34 contemporary American policy issues. My estimate of the between-issue variation in effects is substantively large, plausibly equal to somewhere between one-third and two-thirds the size of the between-study variation observed in the existing literature. This result has important implications for our understanding of party elite influence on public opinion and for the methodological practices of party elite cues research.
Updating amidst Disagreement: New Experimental Evidence on Partisan Cues
Authors: Fowler Anthony , Howell William G 
Published: February 2023

Abstract
In this era of hyper-polarization and partisan animosity, do people incorporate the viewpoints of their political opponents? Perhaps not. An important body of research, in fact, finds that the provision of information about opponents’ policy views leads survey respondents to reflexively adopt the opposite position. In this paper, we demonstrate that such findings arise from incomplete experimental designs and a particular measurement strategy. In a series of experiments that vary information about both parties’ positions simultaneously and that solicit continuous, rather than discrete, policy positions, we find that partisans update their beliefs in accordance with the positions of Republican and Democratic leaders alike. Partisans are not perennially determined to disagree. Rather, they are often willing to incorporate opposing viewpoints about a wide range of policy issues.
WHO should accelerate, not stall, rectal artesunate deployment for pre-referral treatment of severe malaria
Authors: Peto Thomas J , Watson James A , White Nicholas J , Dondorp Arjen M 
Published: February 2023

ABSTRACT
The recent World Health Organization moratorium on rectal artesunate (RAS) for pre-referral treatment of severe childhood malaria is costing young lives. The decision was based on disappointing findings from a large observational study that provided RAS to community health workers with little training and supervision. This non-randomized, operational research has provided useful information to guide the implementation of RAS but is subject to bias and confounding and cannot be used to assess treatment effects. Parenteral artesunate reduces severe malaria mortality and a large body of evidence also shows RAS has lifesaving efficacy. There is now more than a decade of delay in conducting the necessary engagement and training required for successful deployment of RAS. Further delays will result in more preventable deaths.
Genome-Wide Association Study Discovers Novel Germplasm Resources and Genetic Loci with Resistance to Gibberella Ear Rot Caused by Fusarium graminearum
Authors: Yuan Guangsheng , He Dandan , Shi Jiaxin , Li Youliang , Yang Yan , Du Juan , Zou Chaoying , Ma Langlang , Gao Shibin , Pan Guangtang , Shen Yaou 
Published: February 2023

Gibberella ear rot (GER) in maize caused by Fusarium graminearum is one of the most devastating maize diseases reducing grain yield and quality worldwide. The genetic bases of maize GER resistance remain largely unknown. Using artificial inoculation across multiple environments, the GER severity of an association panel consisting of 316 diverse inbred lines was observed with wide phenotypic variation. In the association panel, a genome-wide association study using a general linear model identified 69 single-nucleotide polymorphisms (SNPs) significantly associated with GER resistance at the threshold of 2.04 × 10−5, and the average phenotypic variation explained (PVE) of these SNPs was 5.09%. We also conducted a genome-wide association study analysis using a mixed linear model at a threshold of 1.0 × 10−4, and 16 significantly associated SNPs with an average PVE of 4.73% were detected. A combined general linear model and mixed linear model method obtained 10 co-localized significantly associated SNPs linked to GER resistance, including the most significant SNP (PZE-105079915) with the greatest PVE value, 9.07%, at bin 5.05 following 10 candidate genes. These findings are significant for the exploration of the complicated genetic variations in maize GER resistance. The regions and genes identified herein provide a list of candidate targets for further investigation, in addition to the elite germplasm resources that can be used for breeding GER resistance in maize.
The Expression of Cytoplasmic Effectors by Phytophthora infestans in Potato Leaves and Tubers Is Organ-Biased
Authors: Lacaze Aline , Sormany François , Judelson Howard S. , Joly David L. 
Published: February 2023

Potato late blight is a devastating disease caused by Phytophthora infestans that affects organs, such as leaves and tubers. Previous studies of organ specificity in this interaction have mainly addressed the plant side (i.e., screening for resistance to late blight in potato leaves versus tubers). However, the extent of the organ specificity of P. infestans virulence mechanisms remains understudied. Here, we investigated the extent of organ-specific expression of effector genes using RNA-seq by contrasting the infection of leaves and tubers of potato (cultivar Russet Burbank) by the P. infestans strain 1306. We focused on RxLR and Crinkler (CRN) effectors to obtain insight on genes putatively involved in P. infestans virulence exhibiting differential expression during leaf versus tuber infection. Our results indicated that (i) the proportion of differentially expressed genes (DEGs) increased over time; (ii) a high proportion of effector genes was differentially expressed at one or more time points (34% of RxLRs and 58% of CRNs); and (iii) some RxLR and CRN families appeared to host more DEGs than others. Quantitative reverse transcription PCR (RT-qPCR) was also used to assess the expression of seven RxLR effectors in a time-course experiment, allowing us to validate their organ-biased profiles. Organ-specific effector genes may perform distinct virulence roles at the organ level, which should be considered when performing effectoromics or any infection study. These results bring new insights into the organ specificity of the potato- P. infestans interaction that could lead to improved potato-breeding programs using effectoromics-based approaches. [Formula: see text] Copyright © 2023 The Author(s). This is an open access article distributed under the CC BY-NC-ND 4.0 International license .