bluuebunny/crossref_metadata_2025_split huggingface dataset:
Experimental study on evaluation and optimization of heavy metals adsorption on a novel amidoximated silane functionalized Luffa cylindrica
Authors: Sasan Narkesabad Zahra , Rafiee Reza , Jalilnejad Elham 
Published: March 2023

AbstractThis study aimed to synthesize an amidoximatedLuffa cylindrica(AO-LC) bioadsorbent, and evaluate its efficiency in the adsorption of heavy metals from the aqueous solutions. For this purpose, NaOH solution was used to alkaline treatment ofLuffa cylindrica(LC) fibers. The silane modification of LC was performed using 3-(trimethoxysilyl)propyl methacrylate (MPS). Polyacrylonitrile (PAN)/LC biocomposite (PAN-LC) was synthesized by PAN grafting onto the MPS-modified LC (MPS-LC). Finally, the AO-LC was obtained by the amidoximation of PAN-LC. The chemical structures, morphology, and thermal properties of biocomposites were characterized by the infrared spectroscopy, X-ray diffraction, thermogravimetric analysis, and field emission scanning electron microscopy. The results showed a successful grafting of MPS and PAN on the surface of LC. The order of heavy metals adsorption on AO-LC was: Pb2+ > Ag+ > Cu2+ > Cd2+ > Co2+ > Ni2+. The effects of operational parameters on the Pb2+adsorption were studied using Taguchi experimental design method. Statistical analysis of the results showed that the initial Pb2+concentration and the bioadsorbent dosage significantly affect the adsorption efficiency. The adsorption capacity and removal percentage of Pb2+ions were obtained as 18.88 mg/g and 99.07%, respectively. The Langmuir isotherm and Pseudo-second order kinetics models were found to be better compatible with experimental data as a consequence of the isotherm and kinetics analysis.
Denser forests across the USA experience more damage from insects and pathogens
Authors: Asaro Christopher , Koch Frank H. , Potter Kevin M. 
Published: March 2023

AbstractForests across much of the United States are becoming denser. Trees growing in denser stands experience more competition for essential resources, which can make them more vulnerable to disturbances. Forest density can be expressed in terms of basal area, a metric that has been used to assess vulnerability of some forests to damage by certain insects or pathogens. A raster map of total tree basal area (TBA) for the conterminous United States was compared with annual (2000–2019) survey maps of forest damage due to insects and pathogens. Across each of four regions, median TBA was significantly higher within forest areas defoliated or killed by insects or pathogens than in areas without recorded damage. Therefore, TBA may serve as a regional-scale indicator of forest health and a first filter for identifying areas that merit finer-scale analysis of forest conditions.
Sympathetic neurons secrete retrogradely transported TrkA on extracellular vesicles
Authors: Mason Ashley J. , Keeler Austin B. , Kabir Farah , Winckler Bettina , Deppmann Christopher 
Published: March 2023

AbstractProper wiring of the peripheral nervous system relies on neurotrophic signaling via nerve growth factor (NGF). NGF secreted by target organs (i.e. eye) binds to the TrkA receptor expressed on the distal axons of postganglionic neurons. Upon binding, TrkA is internalized into a signaling endosome and retrogradely trafficked back to the soma and into the dendrites to promote cell survival and postsynaptic maturation, respectively. Much progress has been made in recent years to define the fate of the retrogradely trafficked TrkA signaling endosome, yet it has not been fully characterized. Here we investigate extracellular vesicles (EVs) as a novel route of neurotrophic signaling. Using the mouse superior cervical ganglion (SCG) as a model, we isolate EVs derived from sympathetic cultures and characterize them using immunoblot assays, nanoparticle tracking analysis, and cryo-electron microscopy. Furthermore, using a compartmentalized culture system, we find that TrkA derived from endosomes originating in the distal axon can be detected on EVs secreted from the somatodendritic domain. In addition, inhibition of classic TrkA downstream pathways, specifically in somatodendritic compartments, greatly decreases TrkA packaging into EVs. Our results suggest a novel trafficking route for TrkA: it can travel long distances to the cell body, be packaged into EVs, and be secreted. Secretion of TrkA via EVs appears to be regulated by its own downstream effector cascades, raising intriguing future questions about novel functionalities associated with TrkA+ EVs.
Impact of the Tambora volcanic eruption of 1815 on islands and relevance to future sunlight-blocking catastrophes
Authors: Wilson Nick , Valler Veronika , Cassidy Michael , Boyd Matt , Mani Lara , Brönnimann Stefan 
Published: March 2023

AbstractIsland nations may have potential long-term survival value for humanity in global catastrophes such as sun-blocking catastrophes from nuclear winter and large magnitude volcanic eruptions. One way to explore this issue further is to understand the impact on islands after the largest historically observed volcanic eruption: that of Mt Tambora in 1815. For each of the 31 large, populated islands selected, we conducted literature searches for relevant historical and palaeoclimate studies. We also analysed results from a reconstruction (EKF400v2), which uses atmospheric-only general circulation model simulations with assimilated observational and proxy data. From the literature review, there was widespread evidence for weather/climate anomalies in 1815–1817 for these islands (29/29 for those with data). But missing data was an issue for other dimensions such as impaired food production (seen in 8 islands out of only 12 with data). Based on the EKF400v2 reconstruction for temperature anomalies (compared to the relatively “non-volcanic” reference period of 1779 to 1808), the islands had lower temperature anomalies in the 1815–1818 period than latitudinally equivalent continental sites (at 100 km and 1000 km inland). This was statistically significant for the great majority of the comparisons for group analyses by hemisphere, oceans, and temperate/tropical zone. When considering just the islands, all but four showed statistically anomalous temperature reductions in the 1816–1817 period (for most p < 0.00001). In the peak impact year of 1816, the lowest anomalies were seen for islands in the Southern Hemisphere (p < 0.0001), the Indian Ocean (p < 0.0001), and in the tropics and subtropics of the Southern Hemisphere (p = 0.0057). In conclusion, the findings of both the literature review and reconstruction simulations suggest climatic impacts of the Tambora eruption for nearly all these 31 large islands, albeit less than for continental sites. Islands with the smallest temperature anomalies were in the Southern Hemisphere, in particular the Indian Ocean and the tropics and subtropics of the Southern Hemisphere.
Feasibility and intra-and interobserver reproducibility of quantitative susceptibility mapping with radiomic features for intracranial dissecting intramural hematomas and atherosclerotic calcifications
Authors: Park Sang Ik , Kim Donghyun , Jung Seung Chai , Nam Yoonho , Alabdulwahhab Abdulrahman , Lee Jungbok , Choi Keum Mi 
Published: March 2023

AbstractQuantitative susceptibility mapping (QSM) for 61 patients with dissecting intramural hematomas (n = 36) or atherosclerotic calcifications (n = 25) in intracranial vertebral arteries were collected to assess intra- and interobserver reproducibility in a 3.0-T MR system between January 2015 and December 2017. Two independent observers each segmented regions of interest for lesions twice. The reproducibility was evaluated using intra-class correlation coefficients (ICC) and within-subject coefficients of variation (wCV) for means and concordance correlation coefficients (CCC) and ICC for radiomic features (CCC and ICC > 0.85) were used. Mean QSM values were 0.277 ± 0.092 ppm for dissecting intramural hematomas and − 0.208 ± 0.078 ppm for atherosclerotic calcifications. ICCs and wCVs were 0.885–0.969 and 6.5–13.7% in atherosclerotic calcifications and 0.712–0.865 and 12.4–18.7% in dissecting intramural hematomas, respectively. A total of 9 and 19 reproducible radiomic features were observed in dissecting intramural hematomas and atherosclerotic calcifications, respectively. QSM measurements in dissecting intramural hematomas and atherosclerotic calcifications were feasible and reproducible between intra- and interobserver comparisons, and some reproducible radiomic features were demonstrated.
Impact of implementing emergency demand response program and tie-line on cyber-physical distribution network resiliency
Authors: Osman Sally R. , Sedhom Bishoy E. , Kaddah Sahar S. 
Published: March 2023

AbstractRecently, due to the complex nature of cyber-physical distribution networks (DNs) and the severity of power outages caused by natural disasters, microgrid (MG) formation, distributed renewable energy resources (DRERs), and demand response programs (DRP) have been employed to enhance the resiliency of these networks. This paper proposes a novel multi-objective MGs formation method-based darts game theory optimization algorithm. The microgrid formation is obtained by controlling the sectionalizing and tie-line switches. The network graph theory is used to represent the constructed microgrid, and the non-linear equations of power flow and loss calculations are adopted in the microgrid formation model. To measure the system's resiliency under extreme disaster events, metrics are utilized to prove the system's flexibility and resiliency. The modified IEEE 33-bus test system is designed to validate the proposed approach's effectiveness. Three case studies are performed with and without considering the emergency demand response program (EDRP) and tie-lines.
The importance of high quality real-life social interactions during the COVID-19 pandemic
Authors: Monninger Maximilian , Aggensteiner Pascal-M. , Pollok Tania M. , Kaiser Anna , Reinhard Iris , Hermann Andrea , Reichert Markus , Ebner-Priemer Ulrich W. , Meyer-Lindenberg Andreas , Brandeis Daniel , Banaschewski Tobias , Holz Nathalie E. 
Published: March 2023

AbstractThe coronavirus pandemic has brought about dramatic restrictions to real-life social interactions and a shift towards more online social encounters. Positive social interactions have been highlighted as an important protective factor, with previous studies suggesting an involvement of the amygdala in the relationship between social embeddedness and well-being. The present study investigated the effect of the quality of real-life and online social interactions on mood, and explored whether this association is affected by an individual’s amygdala activity. Sixty-two participants of a longitudinal study took part in a one-week ecological momentary assessment (EMA) during the first lockdown, reporting their momentary well-being and their engagement in real-life and online social interactions eight times per day (N ~ 3000 observations). Amygdala activity was assessed before the pandemic during an emotion-processing task. Mixed models were calculated to estimate the association between social interactions and well-being, including two-way interactions to test for the moderating effect of amygdala activity. We found a positive relationship between real-life interactions and momentary well-being. In contrast, online interactions had no effect on well-being. Moreover, positive real-life social interactions augmented this social affective benefit, especially in individuals with higher amygdala being more sensitive to the interaction quality. Our findings demonstrate a mood-lifting effect of positive real-life social interactions during the pandemic, which was dependent on amygdala activity before the pandemic. As no corresponding effect was found between online social interactions and well-being, it can be concluded that increased online social interactions may not compensate for the absence of real-life social interactions.
Efficacy of resistance training in hypoxia on muscle hypertrophy and strength development: a systematic review with meta-analysis
Authors: Benavente Cristina , Schoenfeld Brad J. , Padial Paulino , Feriche Belén 
Published: March 2023

AbstractA systematic review and meta-analysis was conducted to determine the effects of resistance training under hypoxic conditions (RTH) on muscle hypertrophy and strength development. Searches of PubMed-Medline, Web of Science, Sport Discus and the Cochrane Library were conducted comparing the effect of RTH versus normoxia (RTN) on muscle hypertrophy (cross sectional area (CSA), lean mass and muscle thickness) and strength development [1-repetition maximum (1RM)]. An overall meta-analysis and subanalyses of training load (low, moderate or high), inter-set rest interval (short, moderate or long) and severity of hypoxia (moderate or high) were conducted to explore the effects on RTH outcomes. Seventeen studies met inclusion criteria. The overall analyses showed similar improvements in CSA (SMD [CIs] = 0.17 [− 0.07; 0.42]) and 1RM (SMD = 0.13 [0.0; 0.27]) between RTH and RTN. Subanalyses indicated a small effect on CSA for shorter inter-set rest intervals, moderate hypoxia and moderate loads favoring RTH. Moreover, a medium effect for longer inter-set rest intervals and a trivial to small effect for severe hypoxia and moderate loads favoring RTH was found on 1RM. Evidence suggests that RTH employed with moderate loads (60–80% 1RM) enhances both hypertrophy and strength. Hypertrophy appears to benefit from shorter (≤ 60 s) inter-set rest intervals during RTH while greater gains in strength are achieved with longer rest intervals (≥ 120 s). The use of moderate hypoxia (14.3–16% FiO2) seems to be somewhat beneficial to hypertrophy but not strength. Further research is required with greater standardization of protocols to draw stronger conclusions on the topic.
Nanobubble size distribution measurement by interactive force apparatus under an electric field
Authors: Han Zhenyao , Chen Hao , He Chunlin , Dodbiba Gjergj , Otsuki Akira , Wei Yuezhou , Fujita Toyohisa 
Published: March 2023

AbstractNanobubbles have been applied in many fields, such as environmental cleaning, material production, agriculture, and medicine. However, the measured nanobubble sizes differed among the measurement methods, such as dynamic light scattering, particle trajectory, and resonance mass methods. Additionally, the measurement methods were limited with respect to the bubble concentration, refractive index of liquid, and liquid color. Here, a novel interactive force measurement method for bulk nanobubble size measurement was developed by measuring the force between two electrodes filled with bulk nanobubble-containing liquid under an electric field when the electrode distance was changed in the nm scale with piezoelectric equipment. The nanobubble size was measured with a bubble gas diameter and also an effective water thin film layer covered with a gas bubble that was estimated to be approximately 10 nm based on the difference between the median diameter of the particle trajectory method and this method. This method could also be applied to the solid particle size distribution measurement in a solution.
Genome-wide identification, characterization and expression analysis of AGO, DCL, and RDR families in Chenopodium quinoa
Authors: Yun Shiyu , Zhang Xin 
Published: March 2023

AbstractRNA interference is a highly conserved mechanism wherein several types of non-coding small RNAs regulate gene expression at the transcriptional or post-transcriptional level, modulating plant growth, development, antiviral defence, and stress responses. Argonaute (AGO), DCL (Dicer-like), and RNA-dependent RNA polymerase (RDR) are key proteins in this process. Here, these three protein families were identified inChenopodium quinoa. Further, their phylogenetic relationships with Arabidopsis, their domains, three-dimensional structure modelling, subcellular localization, and functional annotation and expression were analysed. Whole-genome sequence analysis predicted 21CqAGO, eightCqDCL, and 11CqRDRgenes in quinoa. All three protein families clustered into phylogenetic clades corresponding to those of Arabidopsis, including three AGO clades, four DCL clades, and four RDR clades, suggesting evolutionary conservation. Domain and protein structure analyses of the three gene families showed almost complete homogeneity among members of the same group. Gene ontology annotation revealed that the predicted gene families might be directly involved in RNAi and other important pathways. Largely, these gene families showed significant tissue-specific expression patterns, RNA-sequencing (RNA-seq) data revealed that 20CqAGO, sevenCqDCL, and tenCqRDRgenes tended to have preferential expression in inflorescences. Most of them being downregulated in response to drought, cold, salt and low phosphate stress. To our knowledge, this is the first study to elucidate these key protein families involved in the RNAi pathway in quinoa, which are significant for understanding the mechanisms underlying stress responses in this plant.
Biosynthesis of silver nanoparticles using Pseudomonas canadensis, and its antivirulence effects against Pseudomonas tolaasii, mushroom brown blotch agent
Authors: Ghasemi Samira , Harighi Behrouz , Ashengroph Morahem 
Published: March 2023

AbstractThis study reports the biosynthesis of silver nanoparticles (AgNPs) using a Pseudomonas canadensis Ma1 strain isolated from wild-growing mushrooms. Freshly prepared cells of P. canadensis Ma1 incubated at 26–28 °C with a silver nitrate solution changed to a yellowish brown color, indicating the formation of AgNPs, which was confirmed by UV–Vis spectroscopy, scanning electron microscopy (SEM), and X-ray diffraction. SEM analysis showed spherical nanoparticles with a distributed size mainly between 21 and 52 nm, and the XRD pattern revealed the crystalline nature of AgNPs. Also, it provides an evaluation of the antimicrobial activity of the biosynthesized AgNPs against Pseudomonas tolaasii Pt18, the causal agent of mushroom brown blotch disease. AgNPs were found to be bioactive at 7.8 μg/ml showing a minimum inhibitory concentration (MIC) effect against P. tolaasii Pt18 strain. AgNPs at the MIC level significantly reduced virulence traits of P. tolaasii Pt18 such as detoxification of tolaasin, various motility behavior, chemotaxis, and biofilm formation which is important for pathogenicity. Scanning electron microscopy (SEM) revealed that bacterial cells treated with AgNPs showed a significant structural abnormality. Results showed that AgNPs reduced brown blotch symptoms in vivo. This research demonstrates the first helpful use of biosynthesized AgNPs as a bactericidal agent against P. tolaasii.
Factors related to hypermetabolism in individuals with type 2 diabetes mellitus and non-alcoholic fatty liver disease
Authors: Mansour Asieh , Motamed Soudabe , Hekmatdoost Azita , Karimi Sara , Mohajeri-Tehrani Mohammad Reza , Abdollahi Mohammad , Jelodar Reihane , Sajjadi-Jazi Sayed Mahmoud 
Published: March 2023

AbstractConsidering the progressive prevalence and co-occurrence of type 2 diabetes mellitus (T2DM) and non-alcoholic fatty liver disease (NAFLD), as well as the current evidence suggesting the elevated levels of basal metabolic rate (BMR) among these individuals, the present study aimed to identify factors determining hypermetabolism in such subjects. This cross sectional study was conducted in 30 to 53-year-old individuals with concurrent T2DM and NAFLD (controlled attenuation parameter score ≥ 260 dB/m). Resting energy expenditure (REE) was determined by an indirect calorimetry device. Hypermetabolism was defined as an elevated measured REE > 110% of the predicted REE. The multivariate logistic regression test was used for detecting factors associated with hypermetabolism. Between September, 2017, and March, 2018, a total of 95 eligible participants (64.40% male) with both T2DM and NAFLD were included, while 32.63% of them were classified as hypermetabolic. Overall, the mean recruitment age ± standard deviation and median (interquartile range) body mass index were 44.69 ± 5.47 years and 30.20 (27.80–33.30) kg/m2, respectively. Demographic, anthropometric and biochemical variables did not vary significantly across two groups except for total body water, low-density lipoprotein cholesterol and dipeptidyl peptidase 4 (DPP-4) inhibitors (p < 0.05). According to the results of multivariable logistic regression analyses, hypermetabolism had a positive association with adiponectin (odds ratio [OR] 1.167, 95% confidence interval [CI] 1.015–1.342, p = 0.030), physical activity (OR 1.134, 95% CI 1.002–1.284, p = 0.046), alanine transaminase (OR 1.062, 95% CI 1.006–1.122, p = 0.031) and diastolic blood pressure (OR 1.067, 95% CI 1.010–1.127, p = 0.021). However, fat free mass was inversely related to hypermetabolism (OR 0.935, 95% CI 0.883–0.991, p = 0.023). Adiponectin, alanine transaminase, physical activity, diastolic blood pressure and fat free mass were independently associated with hypermetabolism in subjects with NAFLD and T2DM.
Virtual monoenergetic imaging predicting Ki-67 expression in lung cancer
Authors: Dou Peipei , Zhao Hengliang , Zhong Dan , Hu Yingliang , Liu Bin , Zhang Haiyan , Cao Aihong 
Published: March 2023

AbstractThis study aimed to optimize slope and energy levels for evaluating Ki-67 expression in lung cancer using virtual monoenergetic imaging and compare the predictive efficiency of different energy spectrum slopes (λHU) for Ki-67. Forty-three patients with primary lung cancer confirmed via pathological examination were enrolled in this study. They underwent baseline arterial-phase (AP) and venous-phase (VP) energy spectrum computed tomography (CT) scanning before surgery. The CT values were 40–190 keV, with 40–140 keV indicating pulmonary lesions at AP and VP, and P < 0.05 indicating a statistically significant difference. An immunohistochemical examination was conducted, and receiver operating characteristic curves were used to analyze the prediction performance of λHU for Ki-67 expression. SPSS Statistics 22.0 (IBM Corp., NY, USA) was used for statistical analysis, and χ2, t, and Mann–Whitney U tests were used for quantitative and qualitative analyses of data. Significant differences were observed at the corresponding CT values of 40 keV (as 40-keV is considered the best for single-energy image for evaluating Ki-67 expression) and 50 keV in AP and at 40, 60, and 70 keV in VP between high- and low-Ki-67 expression groups (P < 0.05). In addition, the λHU values of three-segment energy spectrum curve in both AP and VP were quite different between two groups (P < 0.05). However, the VP data had greater predictive values for Ki-67. The areas under the curve were 0.859, 0.856, and 0.859, respectively. The 40-keV single-energy sequence was the best single-energy sequence to evaluate the expression of Ki-67 in lung cancer and to obtain λHU values using the energy spectrum curve in the VP. The CT values had better diagnostic efficiency.