bluuebunny/crossref_metadata_2025_split huggingface dataset:
Nutritional evaluation, phytochemical makeup, antibacterial and antioxidant properties of wild plants utilized as food by the Gaddis-a tribal tribe in the Western Himalayas
Authors: Thakur Arti , Singh Somvir , Dulta Kanika , Singh Nitesh , Ali Baber , Hafeez Aqsa , Vodnar Dan C. , Marc Romina Alina 
Published: December 2022

Nowadays, it is generally accepted that medicinal plants play a crucial role in traditional healthcare operations, providing hints to new research fields and in biodiversity protection. However, there is a lack of information on the medicinal uses of plants in many of the interior Himalayan regions. In light of this, the current investigation was initiated in the tribally dominant western Himalayan hinterland. The current study examined five underutilized wild edible plants, namely, Allium rubellum, Berberis chitria, Capsella bursa-pastoris, Stellaria aquatica, and Rheum emodi, for their nutritional qualities, phytochemical analysis, antioxidant activity, and antibacterial activity, which are consumed as food by the Gaddi community of the Bharmour region of the Chamba District, Himachal Pradesh. In this study, the nutritional makeup of these plants was examined in terms of their carbohydrate, protein, sodium, potassium, crude fiber, and fat content. As compared to other investigated species, A. rubellum has the highest nutritional content: carbohydrate (6.93 mg/g), protein (10.18 mg/g), sodium (3.21 mg/g), potassium (16.32 mg/g), and fiber (6.46%). In addition, phenols, amino acids, tannins, terpenoids, carotenoids, and phytate were found to be the least significant phytochemicals in R. emodi, i.e., 4.81 mg/g, 0.594 mg/g, 2.204 mg/g, 1.482 mg/g, 156 µg/g, and 0.680%, respectively. The methanolic extract of these wild edible plants showed significant free radical scavenging activity by using ABTS and DPPH assays. Moreover, the antibacterial activity of the methanolic extract of studied plants based on the present study suggested that R. emodi exhibits a maximum zone of inhibition of 20.8 mm against Escherichia coli, whereas S. aquatica showed a maximum inhibition zone of 19.2 mm against S. aureus. The findings of this study validated that these wild edible plants are an alternate source of medicine and are an abundant source of various phytochemicals like protein, carbohydrates, vitamins, and minerals. These compositions offer dietary benefits, food security, health benefits, and therapeutic advantages. Hence, in the current study, it was analyzed that there is an urgent need for documentation, conservation, characterization, and evaluation of these underutilized plants for their therapeutic purpose and nutritional supplements.
Effect of ApoE ε4 gene polymorphism on the correlation between serum uric acid and left ventricular hypertrophy remodeling in patients with coronary heart disease
Authors: Liu Jia , Zheng Mei-Li , Chen Mulei , Li Kuibao , Zhu Xiaoming , Gao Yuanfeng 
Published: December 2022

BackgroundHyperuricemia and dyslipidemia are associated with left ventricular hypertrophy (LVH), while the effect of ApoE gene polymorphism on the correlation between serum uric acid (UA) level and severity of LVH in patients with coronary heart disease (CHD) has not been clarified.MethodsThis was a retrospective observational study of patients with CHD. Patients were divided into groups of ε4 carriers and non-ε4 carriers based on sanger sequencing. The association of ApoE ε4 gene polymorphism, serum UA level, and LVH, determined by cardiac color Doppler ultrasound, was evaluated by multivariate analysis.ResultsA total of 989 CHD patients who underwent ApoE genotyping were enrolled and analyzed. Among them, the frequency of the ApoE ε4 genotype was 17.9% (15.7% for E3/4, 1.1% for E4/4, and 1.1% for E2/4). There were 159 patients with LVH, 262 with end-diastolic LV internal diameter (LVEDD) enlargement, 160 with left ventricular ejection fraction (LVEF) reduction, and 154 with heart failure. Multivariate analysis showed that for every increase of 10 μmol/L in serum UA level, the risk of LVH decreased in ε4 carriers (odds ratio (OR) = 0.94, 95% confidence interval (CI): 0.890–0.992, P = 0.025) and increased in non-ε4 carriers (OR = 1.03, 95% CI: 1.005–1.049, P = 0.016). The risk of LVEDD enlargement tended to decrease in ε4 carriers (OR = 0.98, 95% CI: 0.943-1.023, P = 0.391) and increased in non-ε4 carriers (OR = 1.03, 95% CI: 1.009–1.048, P = 0.003). The risk of LVEF reduction was reduced in ε4 carriers (OR = 0.996, 95% CI: 0.949–1.046, P = 0.872) and increased in non-ε4 carriers (OR = 1.02, 95% CI: 0.994–1.037, P = 0.17). The risk of LVEDD enlargement decreased in ε4 carriers (OR = 0.98, 95% CI: 0.931–1.036, P = 0.508) and increased in non-ε4 carriers (OR = 1.02, 95% CI: 0.998–1.042, P = 0.07).ConclusionHigh serum UA levels decreased the risk of LVH in ApoE ε4 carriers with CHD, while increased the risk of LVH in non-ε4 carriers.
Schiff bases complexed with iron and their relation with the life cycle and infection by Schistosoma mansoni
Authors: da Silva Juliana Virginio , Moreira Carla Cristina , Montija Elisandra de Almeida , Feitosa Karina Alves , Correia Ricardo de Oliveira , Domingues Nelson Luis de Campos , Soares Edson Garcia , Allegretti Silmara Marques , Afonso Ana , Anibal Fernanda de Freitas 
Published: December 2022

IntroductionThe trematode Schistosoma mansoni causes schistosomiasis, and this parasite’s life cycle depends on the mollusk Biomphalaria glabrata. The most effective treatment for infected people is administering a single dose of Praziquantel. However, there are naturally resistant to treatment. This work has developed, considering this parasite’s complex life cycle.MethodsThe synthetics compound were evaluated: i) during the infection of B. glabrata, ii) during the infection of BALB/c mice, and iii) during the treatment of mice infected with S. mansoni.Results and DiscussionFor the first objective, snails infected with miracidia treated with compounds C1 and C3 at concentrations of 25% IC50 and 50% IC50, after 80 days of infection, released fewer cercariae than the infected group without treatment. For the second objective, compounds C1 and C3 did not show significant results in the infected group without treatment. For the third objective, the mice treated with C3 and C1 reduced the global and differential cell count. The results suggest that although the evaluated compounds do not present schistosomicidal properties when placed in cercariae suspension, they can stimulate an immune reaction in snails and decrease mice’s inflammatory response. In general, we can conclude that compound C1 and C3 has an anti-schistosomicidal effect both in the larval phase (miracidia) and in the adult form of the parasite.
Polo-like kinase 1 is related with malignant characteristics and inhibits macrophages infiltration in glioma
Authors: Luo Lin , Zhang Xiao-Yang , Zhen Ying-Wei , Guo Gao-Chao , Peng Da-Zhao , Wei Cheng , Pei Dong-Ling , Yu Bin , Ji Yu-Chen , Liu Xian-Zhi , Han Lei , Zhang Zhen-Yu 
Published: December 2022

BackgroundTumor immune microenvironment (TIM) plays a critical role in tumorigenesis and progression. Recently, therapies based on modulating TIM have made great breakthroughs in cancer treatment. Polo-like kinase 1 (PLK1) is a crucial regulatory factor of the cell cycle process and its dysregulations often cause various pathological processes including tumorigenesis. However, the detailed mechanisms surrounding the regulation of PLK1 on glioma immune microenvironment remain undefined.MethodsPublic databases and online datasets were used to extract data of PLK1 expression, clinical features, genetic alterations, and biological functions. The EdU, flow cytometry, and macrophage infiltration assays as well as xenograft animal experiments were performed to determine the relationship between PLK1 and glioma immune microenvironment in vivo and in vitro.ResultsPLK1 is always highly expressed in multiple cancers especially in glioma. Univariable and Multivariate proportional hazard Cox analysis showed that PLK1 was a prognostic biomarker for glioma. Simultaneously, highly expressed PLK1 is significantly related to prognosis, histological and genetic features in glioma by analyzing public databases. In addition, the enrichment analysis suggested that PLK1 might related to “immune response”, “cell cycle”, “DNA replication”, and “mismatch repair” in glioma. Immune infiltration analysis demonstrated that highly expressed PLK1 inhibited M1 macrophages infiltration to glioblastoma immune microenvironment by Quantiseq and Xcell databases and negatively related to some chemokines and marker genes of M1 macrophages in glioblastoma. Subsequent experiments confirmed that PLK1 knockdown inhibited the proliferation of glioma cells but increased the M1 macrophages infiltration and polarization. Furthermore, in glioma xenograft mouse models, we showed that inhibiting PLK1 blocked tumor proliferation and increased the M1 macrophages infiltration. Finally, PLK1 methylation analysis and lncRNA-miRNA network revealed the potential mechanism of abnormal PLK1 expression in glioma.ConclusionsPLK1 inhibits M1 macrophages infiltration into glioma immune microenvironment and is a potential biomarker for glioma.
Efficacy of interoceptive and embodied rehabilitative training protocol in patients with mild multiple sclerosis: A randomized controlled trial
Authors: Paolucci Teresa , de Sire Alessandro , Agostini Francesco , Bernetti Andrea , Salomè Angela , Altieri Marta , Di Piero Vittorio , Ammendolia Antonio , Mangone Massimiliano , Paoloni Marco 
Published: December 2022

IntroductionThe aim of this randomized controlled trial was to evaluate the effect of an embodied rehabilitative protocol, in improving interoceptive awareness respect balance and motor performance in patients with mild multiple sclerosis (pwMS).MethodsIn this study patients with relapsing-remitting multiple sclerosis were enrolled. The rehabilitative treatment group (TG) participated in an embodied physiotherapy program consisting of 8 one-hour sessions in groups of 4 patients at a time, 1 per week and 2 one-hour sessions of neuro-cognitive exercise in single session during the rehabilitation program. All pwMS underwent a clinical assessment to measure the interoception sense for the Multidimensional Assessment of Interoceptive Awareness scale, balance for the Tinetti Mobility test and stabilometry, quality of life for the Short Form Health Survey-12 and body image perception for Trunk Appearance Perception Scale and Body Image Scale. All previous scales and tests were performed at baseline (T0), at the end of treatment (T1) and after 2 months of follow up (T2).ResultsSixty patients were enrolled and randomized into two groups: TG (n = 30), aged 43.0 ± 10.2 years, and a control/waiting list (WLG) group (n = 30), aged 40.7 ± 10.4 years. Statistically significant improvements in interoceptive awareness, body image perception, balance and quality of life were reported in TG versus WLG (p &lt; 0.05).DiscussionThis study suggests that enhancing interoceptive awareness could improve postural balance. Future studies with a larger sample of patients will be needed to better quantify the effects of an embodied rehabilitation.
Transcutaneous spinal stimulation in patients with intrathecal baclofen pump delivery system: A preliminary safety study
Authors: Lopez John , Forrest Gail F. , Engel-Haber Einat , Snider Brittany , Momeni Kam , Ravi Manikandan , Kirshblum Steven 
Published: December 2022

ObjectiveTo determine the effect of transcutaneous spinal stimulation (TSS) on an implanted intrathecal baclofen (ITB) pump in persons with traumatic spinal cord injury (SCI).DesignProspective clinical trial.ParticipantsFive individuals with chronic traumatic SCI, &gt;18 years of age, and an anteriorly implanted Medtronic SynchroMed™ II ITB pump delivery system.InterventionTranscutaneous spinal stimulation trials with cathode at T11/12, with pump interrogation before, during and after stimulation.ResultsThere was no evidence of any effect of the TSS in regards to disruption of the ITB pump delivery mechanism. Communication interference with the interrogator to the pump occurred often during stimulation for log transmission most likely secondary to the electromagnetic interference from the stimulation. One individual had elevated blood pressure at the end of the trial, suspected to be unrelated to the spinal stimulation.ConclusionBased upon this pilot study, further TSS studies including persons with an implanted Medtronic SynchroMed™ II ITB pump can be considered when stimulating at the low thoracic spine, although communication with the programmer during the stimulation may be affected.
Improved detection of coronary artery disease by CZT regional coronary blood flow evaluation
Authors: Lima R. S. L. , Bezerra A. , Andrade M. , Domenico C. , De Lorenzo A. 
Published: December 2022

IntroductionCZT cameras have enabled the noninvasive quantification of myocardial flow reserve (MFR), an important physiologic measure. This study aimed to compare myocardial perfusion SPECT (MPS) with or without MFR evaluation for the detection of obstructive coronary artery disease (CAD).Methods48 patients with CAD (&gt;50% obstruction) detected at invasive coronary angiography or CT angiography underwent dipyridamole MPS and MFR evaluation within 30 days. A 1-day protocol (rest-stress) was used to quantify MFR. The acquisition of dynamic rest and stress images was initiated simultaneously to 99mTc sestamibi injection (370 and 1,110 MBq, respectively), both lasting for 11 min, followed by 5-min imaging. Pharmacologic stress with dipyridamole (0.56 mg/kg for 4 min) was performed with the patient positioned in the CZT camera. The images were processed and time-activity curves were generated, calculating global and regional MFR in a semiautomatic software. A global or regional MFR &lt;2 was considered abnormal. MPS perfusion images were classified as normal or abnormal. The images were interpreted by experienced physicians blinded to the results of MFR and coronary angiography/CT.ResultsMean age of the population was 61 ± 9 years, 54.2% female. Twenty patients (41.7%) had single-vessel CAD, 22 (45.8%) 2-vessel CAD and 6 (12.5%), triple-vessel CAD. Among the 82 vessels with obstruction, 48 had perfusion abnormalities in MPS and 60 had reduced MFR, while among the normal vessels, had 54 normal MPS and 52 had preserved MFR. The sensitivity of MFR (69%) was higher than that of MPS (55.2%), without significant changes in specificity (86 vs. 83.7%).ConclusionsMFR in the CZT camera is more sensitive for the detection of CAD than perfusion abnormalities in MPS, especially in patients with multivessel CAD.
Drug repositioning based on heterogeneous networks and variational graph autoencoders
Authors: Lei Song , Lei Xiujuan , Liu Lian 
Published: December 2022

Predicting new therapeutic effects (drug repositioning) of existing drugs plays an important role in drug development. However, traditional wet experimental prediction methods are usually time-consuming and costly. The emergence of more and more artificial intelligence-based drug repositioning methods in the past 2 years has facilitated drug development. In this study we propose a drug repositioning method, VGAEDR, based on a heterogeneous network of multiple drug attributes and a variational graph autoencoder. First, a drug-disease heterogeneous network is established based on three drug attributes, disease semantic information, and known drug-disease associations. Second, low-dimensional feature representations for heterogeneous networks are learned through a variational graph autoencoder module and a multi-layer convolutional module. Finally, the feature representation is fed to a fully connected layer and a Softmax layer to predict new drug-disease associations. Comparative experiments with other baseline methods on three datasets demonstrate the excellent performance of VGAEDR. In the case study, we predicted the top 10 possible anti-COVID-19 drugs on the existing drug and disease data, and six of them were verified by other literatures.
The estimation of broiler respiration rate based on the semantic segmentation and video amplification
Authors: Wang Jintao , Liu Longshen , Lu Mingzhou , Okinda Cedric , Lovarelli Daniela , Guarino Marcella , Shen Mingxia 
Published: December 2022

Respiratory rate is an indicator of a broilers’ stress and health status, thus, it is essential to detect respiratory rate contactless and stress-freely. This study proposed an estimation method of broiler respiratory rate by deep learning and machine vision. Experiments were performed at New Hope (Shandong Province, P. R. China) and Wen’s group (Guangdong Province, P. R. China), and a total of 300 min of video data were collected. By separating video frames, a data set of 3,000 images was made, and two semantic segmentation models were trained. The single-channel Euler video magnification algorithm was used to amplify the belly fluctuation of the broiler, which saved 55% operation time compared with the traditional Eulerian video magnification algorithm. The contour features significantly related to respiration were used to obtain the signals that could estimate broilers’ respiratory rate. Detrending and band-pass filtering eliminated the influence of broiler posture conversion and motion on the signal. The mean absolute error, root mean square error, average accuracy of the proposed respiratory rate estimation technique for broilers were 3.72%, 16.92%, and 92.19%, respectively.
Wrist-Worn devices to encourage affected upper limb movement in unilateral cerebral palsy: Participatory design workshops
Authors: Brown Rebekah , Pearse Janice Elizabeth , Nappey Tom , Jackson Dan , Edmonds Grace , Guan Yu , Basu Anna Purna 
Published: December 2022

BackgroundUnilateral (Hemiplegic) cerebral palsy (UCP) causes weakness and stiffness affecting one sided of the body, often impacting activities of daily living. Upper limb therapy at effective intensity is not accessible to most.AimTo determine stakeholder views on design of an approach using wrist-worn devices and a smartphone application to encourage use of the affected upper limb for children with hemiplegia.MethodFour participatory design workshops and one young people's advisory group workshop incorporating views of five young people with hemiplegia, 13 typically developing peers aged 8–18 years, four parents, three occupational therapists, one teacher and two paediatricians. Two special educational needs co-ordinators were consulted separately. Peers were included to explore a study design whereby each child with hemiplegia would have a participating “buddy”. Topics included views on an acceptable wrist-worn device and smartphone application, participant age range, involvement of a buddy, and barriers to using the technology in a school setting. Ethical/welfare considerations included data security, and potential risks around providing smartphones to young children.ResultsChildren wanted a comfortable, conventional-appearing wristband incorporating a watch face and a secure, well-fitting strap. They were prepared to wear a band on each wrist. They wanted support with explaining the study to schoolteachers. Most schools restricted smartphone use during the school day: the study design accommodated this. Children agreed with a game as reward but had different views on an acceptable game; direct access to feedback data was preferred by some. Parents commented on the lack of access to upper limb therapy for children with UCP; therapists concurred. The proposed participant age range was widened based on feedback. Typically developing children were prepared to be buddies to help a friend with CP. Stakeholders were reassured by data security explanations and plans to provide internet safety information to participants.ConclusionThe participatory design process informed plans for the proof-of-concept stage of the study, hopefully leading to an approach that will be fun, easy to integrate into everyday life, and have the capacity to increase use of the affected arm and hand.
A Nationwide Study of Inpatient Case Rate Incidence of Cannabis-Related Diagnoses in Switzerland
Authors: Pfeifer Philippe , Auer Reto , Baggio Stéphanie , Moggi Franz 
Published: December 2022

Objectives: Cannabis is the most commonly used illicit substance and has been associated with mental health issues. In this study, we examined trends in hospitalizations due to cannabis use.Methods: Data were obtained from the Swiss Federal Statistics Office and comprised hospital main diagnoses, gender, age group and region of all psychiatric inpatient cases in Switzerland from 1998 to 2020. We performed trend analyses of annual case rates with cannabinoid-related diagnoses and compared them to trend analyses of alcohol-related and psychotic disorders.Results: Case rates of CRDs significantly increased in the observed time period. From all psychiatric main diagnoses, CRDs were overrepresented in the age groups of 15–24 and 25–44 years.Conclusion: We found a sharp increase in hospitalizations for CRD. Future studies should test whether changes in the upcoming cannabis regulation, which can facilitate the implementation of interventions to address mental health among users, impact future hospitalization rates of CRD.
Function of Motives in Karawo Gorontalo Embroidery Fabric
Authors: Rumambie Wira Pratama , Djalari Yusuf Affendi 
Published: December 2022

The purpose of this research is to see and categorize some of the functions of the Karawo embroidered cloth seeing from the motifs in Goronalo, Karawo itself is a typical traditional cloth originating from Gorontalo whose manufacture is produced by handicrafts. Karawo itself has a unique way of doing it, such as making karawo which requires patience and thoroughness, making karawo takes one week to make one karawo that is ready to use. To support the research, we use descriptive methods and the data collected in the preparation of journals are analyzed descriptively, data collection is also in the form of interview data and study results from literature sources.
The results show that Gorontalo karawo embroidery has its own meaning to the function of the motifs that will be used as a complementary ornament of the cloth so that the karawo embroidery is formed. This is because each of the motifs continues to develop and shift to a more modern direction which makes the karawo motif in a certain function has its own meaning for its users which is then adjusted to what the consumer likes. However, this can add to the function of the more modern karawo motifs as time goes by which then choose to leave Gorontalo customary and cultural motifs. The conclusion of the research is to find that the karawo embroidery handicraft has a function of every motif that has been made, from traditional cultural motifs to modern motifs that tend to be liked or interested by users.
Keyword: Culture; Embriodery; Gorontalo; Karawo; John and Fritz Koraag
Jaroslav Hašek, Las aventuras del buen soldado Švejk, y la consolidación de una identidad nacional
Authors: Torrada Alfredo Martín 
Published: December 2022

Si bien los puntos de contacto entre literatura e identidad son múltiples y constantes, ningún otro libro logró, tanto como Las aventuras del buen soldado Švejk, de Jaroslav Hašek, erigirse como una obra en la cual el pueblo checo se viera reflejado. El presente trabajo ahonda en las razones por las cuales la novela de Hašek ha conseguido constituirse en un símbolo nacional. Su discurso antiimperial, el uso de personajes plebeyos y la construcción de una literatura al ras del suelo, la constante mirada hacia el interior de su pueblo, y la escritura producida directamente desde el caos de las tabernas, son algunos de los puntos que este artículo analiza.