bluuebunny/crossref_metadata_2025_split huggingface dataset:
Differences in Survival across Monogenic Forms of Parkinson's Disease
Authors: Lanore Aymeric , Casse Fanny , Tesson Christelle , Courtin Thomas , Menon Poornima Jayadev , Sambin Sara , Mangone Graziella , Mariani Louise‐Laure , Lesage Suzanne , Brice Alexis , Elbaz Alexis , Corvol Jean‐Christophe ,   for the French Clinicians Network for Parkinson's Disease Genetics (the PDG Group)
Published: March 2023

ObjectiveSurvival of patients with monogenic Parkinson's disease may depend on the causative genes associated with the disease. In this study, we compare survival of patients with Parkinson's disease according to the presence of SNCA, PRKN, LRRK2, or GBA mutations.MethodsData from the French Parkinson Disease Genetics national multicenter cohort study were used. Patients with sporadic and familial Parkinson's disease were recruited between 1990 and 2021. Patients were genotyped for the presence of mutations in the SNCA, PRKN, LRRK2, or GBA genes. Vital status was collected from the National death register for participants born in France. Hazard ratios (HRs) and 95% confidence intervals (CIs) were computed using multivariable Cox proportional hazards regression.ResultsOf the 2,037 patients with Parkinson's disease, 889 had died after a follow‐up of up to 30 years. Patients with PRKN (n = 100, HR = 0.41; p = 0.001) and LRRK2 mutations (n = 51, HR = 0.49; p = 0.023) had longer survival than those without any mutation, whereas patients with SNCA (n = 20, HR = 9.88; p < 0.001) or GBA mutations (n = 173, HR = 1.33; p = 0.048) had shorter survival.InterpretationSurvival differs across genetic forms of Parkinson's disease, with higher mortality for patients with SNCA or GBA mutations, and lower mortality for those with PRKN or LRRK2 mutations. Differences in severity and disease progression among monogenic forms of Parkinson's disease likely explain these findings, which has important consequences for genetic counselling and choice of end points for future clinical trials for targeted therapies. ANN NEUROL 2023;94:123–132
An Efficient Opal‐Suppressor Tryptophanyl Pair Creates New Routes for Simultaneously Incorporating up to Three Distinct Noncanonical Amino Acids into Proteins in Mammalian Cells**
Authors: Osgood Arianna O. , Zheng Yunan , Roy Soumya Jyoti Singha , Biris Nikolaos , Hussain Myer , Loynd Conor , Jewel Delilah , Italia James S. , Chatterjee Abhishek 
Published: March 2023

AbstractSite‐specific incorporation of multiple distinct noncanonical amino acids (ncAAs) into proteins in mammalian cells is a promising technology, where each ncAA must be assigned to a different orthogonal aminoacyl‐tRNA synthetase (aaRS)/tRNA pair that reads a distinct nonsense codon. Available pairs suppress TGA or TAA codons at a considerably lower efficiency than TAG, limiting the scope of this technology. Here we show that the E. coli tryptophanyl (EcTrp) pair is an excellent TGA‐suppressor in mammalian cells, which can be combined with the three other established pairs to develop three new routes for dual‐ncAA incorporation. Using these platforms, we site‐specifically incorporated two different bioconjugation handles into an antibody with excellent efficiency, and subsequently labeled it with two distinct cytotoxic payloads. Additionally, we combined the EcTrp pair with other pairs to site‐specifically incorporate three distinct ncAAs into a reporter protein in mammalian cells.
Precise Regulation of Emission Maxima and Construction of Highly Efficient Electroluminescent Materials with High Color Purity
Authors: Wang Qingyang , Xu Yincai , Huang Tingting , Qu Yupei , Xue Jianan , Liang Baoyan , Wang Yue 
Published: March 2023

AbstractAdvanced multiple resonance induced thermally activated delayed fluorescence (MR‐TADF) emitters have emerged as a privileged motif for applications in organic light‐emitting diodes (OLEDs), because they furnish highly tunable TADF characteristics and high color purity emission. Herein, based on the unique nitrogen‐atom embedding molecular engineering (NEME) strategy, a series of compounds BN‐TP‐Nx (x=1, 2, 3, 4) have been customized. The nitrogen‐atom anchored at different position of triphenylene hexagonal lattice entails varying degrees of perturbation to the electronic structure. The newly‐constructed emitters have demonstrated the precise regulation of emission maxima of MR‐TADF emitters to meet the actual industrial demand, and further enormously enriched the MR‐TADF molecular reservoir. The BN‐TP‐N3‐based OLED exhibits ultrapure green emission, with peak of 524 nm, full‐width at half‐maximum (FWHM) of 33 nm, Commission Internationale de L'Eclairage (CIE) coordinates of (0.23, 0.71), and maximum external quantum efficiency (EQE) of 37.3 %.
GdIII‐19F Distance Measurements for Proteins in Cells by Electron‐Nuclear Double Resonance
Authors: Seal Manas , Zhu Wenkai , Dalaloyan Arina , Feintuch Akiva , Bogdanov Alexey , Frydman Veronica , Su Xun‐Cheng , Gronenborn Angela M. , Goldfarb Daniella 
Published: March 2023

AbstractStudies of protein structure and dynamics are usually carried out in dilute buffer solutions, conditions that differ significantly from the crowded environment in the cell. The double electron‐electron resonance (DEER) technique can track proteins’ conformations in the cell by providing distance distributions between two attached spin labels. This technique, however, cannot access distances below 1.8 nm. Here, we show that GdIII‐19F Mims electron‐nuclear double resonance (ENDOR) measurements can cover part of this short range. Low temperature solution and in‐cell ENDOR measurements, complemented with room temperature solution and in‐cell GdIII‐19F PRE (paramagnetic relaxation enhancement) NMR measurements, were performed on fluorinated GB1 and ubiquitin (Ub), spin‐labeled with rigid GdIII tags. The proteins were delivered into human cells via electroporation. The solution and in‐cell derived GdIII‐19F distances were essentially identical and lie in the 1–1.5 nm range revealing that both, GB1 and Ub, retained their overall structure in the GdIII and 19F regions in the cell.
Singlet oxygen quenching activity of furan fatty acids: Kinetic and thermodynamics study
Authors: Seki Kensuke , Okada Youji 
Published: March 2023

AbstractWe have undertaken a detailed study of the reactivity of furan fatty acids (F‐acids) with singlet oxygen (1O2) produced by thermal decomposition of endoperoxide and photosensitizer reaction of rose bengal in ethanol. Accordingly, we measured the second order rate constant (kQ) for the reaction of F‐acids with 1O2. As a result, the reactivity of F‐acids against 1O2 exhibited a decrease in the order tetra‐alkyl F‐acid (F3) > tri‐alkyl F‐acid (F2) > di‐alkyl F‐acid (NMF). In addition, we also employed the density functional theory (DFT) and quantum chemical calculations to predict the chemical reactivity, stability, and thermodynamic properties of F‐acids with respect to the quenching 1O2. In consequence, it is clear that log kQ values of F‐acids correlate with the energy level of the highest occupied molecular orbital (EHOMO), and HOMO of F‐acids may react with the lowest unoccupied molecular orbitals (LUMO) of 1O2. Moreover, the reaction of F‐acids with 1O2 proceeds spontaneously in ethanol based on the calculation by thermodynamics method. These results suggest that the F‐acids may contribute to the protection of oxidative damage in animals by quenching 1O2.
Synthesis, characterization, and tailoring variations in the linear optical of erbium‐ions doped (PVA/PVP) polymeric composites for optical devices: Improvement of the dielectric characteristics
Authors: Mohammed M. I. , Yahia I. S. , El‐Mongy S. Abd 
Published: March 2023

AbstractThe simple solution casting method was utilized for preparing composite films (PVA‐30 wt% PVP) incorporated with various content of erbium ions (Er3+) (0.11, 0.37, 1.85, 3.7, 9.25, and 18.5) wt%. The composite films' properties were investigated using XRD, UV–Vis, and dielectric spectrometers. The XRD patterns showed a noticeable decline in structural elements, including crystallinity level. The transmittances of the investigated samples decrease as Er3+ increases. Studies on UV–Vis absorption have revealed that the UV–Vis absorption spectra are shifting to the higher wavelengths, causing a decrease in the optical energy bandgap values from 4.66 to 3.48 eV for an indirect transition and from 5 to 4.84 eV for a direct transition, which suggests that the filler interacts with the blend matrix. As the number of localized states in the forbidden energy bandgap rises, it has been shown that with increasing Er3+‐ the estimated Urbach's energy increases from 1.95 to 32 meV. The addition of erbium ions raises the refractive index of the blend film. The optical limits of the samples are investigated using a He‐Ne laser beam with a wavelength of 632.8 nm and a solid‐state green diode laser beam with a wavelength of 532 nm. The prospect of greater charge carrier concentration and mobility was confirmed by increased AC conductivity with increasing frequency. Dielectric permittivity and dielectric loss tangent were utilized to analyze dielectric behavior. The analysis of the electric modulus (M′ and M′′) of as‐prepared samples has been completed. These findings are anticipated to notably impact a broad spectrum of applications, including organic semiconductors, optoelectronic devices, polymer solar cells, and polymer waveguides.
Development and validation of a machine learning‐based tool to predict autism among children
Authors: Betts Kim Steven , Chai Kevin , Kisely Steve , Alati Rosa 
Published: March 2023

AbstractAutism is a lifelong condition for which intervention must occur as early as possible to improve social functioning. Thus, there is great interest in improving our ability to diagnose autism as early as possible. We take a novel approach to this challenge by combining machine learning with maternal and infant health administrative data to construct a prediction model capable of predicting autism disorder (defined as ICD10 84.0) in the general population. The sample included all mother–offspring pairs from the Australian state of New South Wales (NSW) between January 2003 and December 2005 (n = 262,650 offspring), linked across three health administrative data sets including the NSW perinatal data collection (PDC); the NSW admitted patient data collection (APDC) and the NSW mental health ambulatory data collection (MHADC). Our most successful model was able to predict autism disorder with an area under the receiver operating curve of 0.73, with the strongest risk factors for diagnoses found to include offspring gender, maternal age at birth, delivery analgesia, maternal prenatal tobacco disorders, and low 5‐min APGAR score. Our findings indicate that the combination of machine learning and routinely collected admin data, with further refinement and increased accuracy than achieved by us, may play a role in the early detection of autism disorders.
Effects of ketamine‐induced H3K9 hypoacetylation during pregnancy on cardiogenesis of mouse offspring
Authors: Yu Yujuan , Quan Junjun , Zou Mou , Zhao Wei , Su Yujuan , Xu Ying 
Published: March 2023

AbstractBackgroundPrenatal exposure to adverse factors can cause congenital heart defects. Ketamine, a widely used anesthetic drug, produces several adverse reactions such as tachycardia, hypertension, and laryngospasm, especially in pediatric patients. This study aimed to detect the effects of ketamine exposure during pregnancy on the cardiogenesis of mouse offspring and the potential mechanisms.MethodsIn this study, ketamine at an addictive dose (5 mg/kg) was administered to mice during early gestation to explore the epigenetic mechanism of its causing cardiac dysplasia. The cardiac morphology of the mouse offspring was observed through hematoxylin–eosin staining and transmission electron microscopy. The heart function of one‐month‐old neonates was detected by echocardiography. The expression of cardiomyogenesis‐related genes was detected by western blot and RT‐qPCR. The acetylation level of histone H3K9 at the Mlc2 promoter and its deacetylase level and activity were detected by CHIP‐qPCR, RT‐qPCR, and ELISA, respectively.ResultsOur data revealed that ketamine exposure during pregnancy could cause cardiac enlargement, myocardial sarcomere disorganization, and decreased cardiac contractile function in mouse offspring. Moreover, ketamine reduced the expression of Myh6, Myh7, Mlc2, Mef2c, and cTnI. The histone H3K9 acetylation level at the Mlc2 promoter was down‐regulated by increasing the histone deacetylase activity and HDAC3 level upon ketamine administration.ConclusionsOur work indicates that H3K9 acetylation is a vital player in cardiac dysplasia in offspring caused by prenatal ketamine exposure and HDAC3 is a key regulatory factor.
Modern slavery and the Global Reporting Initiative – A bridge too far?
Authors: Burritt Roger Leonard , Christ Katherine Leanne 
Published: March 2023

AbstractAs the Global Reporting Initiative currently provides the most widely used set of voluntary sustainability reporting standards, the question arises as to the extent to which the Initiative's multi‐stakeholder governance is helping towards ending modern slavery in line with the United Nations Sustainable Development Goal 8. Stakeholder theory and examination of the Initiative's sustainability standards are used, to examine the issue. Evidence from the Initiative's set of universal, topic and sector standards reveals that, while forced and child labour are identified as granular material topics for sustainability reporting, the broader concept of modern slavery has not been recognised as a required material theme for disclosure. One recent exception is voluntary publication of three new sector standards, where the modern slavery term appears to be introduced as a symbolic rather than a substantive notion. Main contributions from the research relate to, first, providing a critique of the multi‐stakeholder foundations for governance as used by the Initiative; second, examination of the results of the Initiative's standard setting process in terms of its standards and their incorporation of modern slavery, from a multi‐stakeholder governance perspective with the hope of improving governance in the future.
Risk, resilience, and recovery in forensic mental health: An integrated conceptual model
Authors: Penney Stephanie R. , Faziluddin Suraya , Simpson Alexander I. F. , Socha Patti , Wilkie Treena 
Published: March 2023

AbstractIn this paper we describe a novel, integrated conceptual model that brings together core elements across structured tools assessing risk for future violence, protective factors, and progress in treatment and recovery in forensic mental health settings. We argue that the value of such a model lies in its ability to improve clinical efficiencies and streamline assessment protocols, facilitate meaningful participation of patients in assessment and treatment planning activities and increase the accessibility of clinical assessments to principal users of this information. The four domains appearing in the model (treatment engagement, stability of illness and behavior, insight, and professional and personal support) are described, and common clinical manifestations of each domain within a forensic context are illustrated. We conclude with a discussion of the types of research that would be needed to validate a concept model such as the one presented here as well as implications for clinical practice and implementation.
Sex‐specific bone and muscular morphological features in ischiofemoral impingement: A three‐dimensional study
Authors: Wang Yining , Liang Wenjie , Chen Yanjun , Li ShaoLin , Ji Hongli , Feng Zhengkuan , Ma Dong , Zhong Shizhen , Ouyang Jun , Qian Lei 
Published: March 2023

AbstractThe study aimed to investigate how hip bone and muscular morphology features differ between ischiofemoral impingement (IFI) patients and healthy subjects among males and females. Three‐dimensional models were reconstructed based on magnetic resonance imaging images from IFI patients and healthy subjects of different sexes. Bone morphological parameters and the cross‐sectional area of the hip abductors were measured. The diameter and angle of the pelvis were compared between patients and healthy subjects. Bone parameters of the hip and cross‐sectional area of the hip abductors were compared between affected and healthy hips. The comparison results of some parameters were significant for females but not males. For females, the comparison results of pelvis parameters showed that the anteroposterior diameter of the pelvic inlet (p = 0.001) and intertuberous distance (p < 0.001) were both larger in IFI patients than in healthy subjects. Additionally, the comparison results of hip parameters showed that the neck shaft angle (p < 0.001) and the cross‐sectional area of the gluteus medius (p < 0.001) and gluteus minimus (p = 0.005) were smaller, while the cross‐sectional area of the tensor fasciae latae (p < 0.001) was significantly larger in affected hips. Morphological changes in IFI patients demonstrated sexual dimorphism, including bone and muscular morphology. Differences in the anteroposterior diameter of the pelvic inlet, intertuberous distance, neck shaft angle, gluteus medius, and gluteus minimus may explain why females are more susceptible to IFI.
Development of the McMaster Embalming Scale (MES) to assess embalming solutions for surgical skills training
Authors: Wang Austine , de SA Darren , Darie Sorin , Zhang Betty , Rockarts Jasmine , Palombella Andrew , Nguyen Laura , Downer Naomi , Wainman Bruce , Monteiro Sandra 
Published: March 2023

AbstractHuman cadavers used for surgical training are embalmed using various methods to facilitate tissue storage and longevity while preserving the natural characteristics required to achieve high fidelity functional task alignment. However, there are no standardized means to evaluate the suitability of embalming solutions for this purpose. The McMaster Embalming Scale (MES) was developed to assess the extent to which embalming solutions allow tissues to achieve physical and functional correspondence to clinical contexts. The MES follows a five‐point Likert scale format and evaluates the effect of embalming solutions on tissue utility in seven domains. This study aims to determine the reliability and validity of the MES by presenting it to users after performing surgical skills on tissues embalmed using various solutions. A pilot study of the MES was conducted using porcine material. Surgical residents of all levels and faculty were recruited via the Surgical Foundations program at McMaster University. Porcine tissue was unembalmed (fresh‐ frozen) or embalmed using one of seven solutions identified in the literature. Participants were blinded to the embalming method as they completed four surgical skills on the tissue. After each performance, participants evaluated their experience using the MES. Internal consistency was evaluated using Cronbach's alpha. Domain to total correlations and a g‐study were also conducted. Formalin‐fixed tissue achieved the lowest average scores, while fresh frozen tissue achieved the highest. Tissues preserved using Surgical Reality Fluid (Trinity Fluids, LLC, Harsens Island, MI) achieved the highest scores among embalmed tissues. The Cronbach's alpha scores varied between 0.85 and 0.92, indicating a random sample of new raters would offer similar ratings using the MES. All domains except odor were positively correlated. The g‐study indicated that the MES is able to differentiate between embalming solutions, but an individual rater's preference for certain tissue qualities also contributes to the variance in scores captured. This study evaluated the psychometric characteristics of the MES. Future steps to this investigation include validating the MES on human cadavers.
Lindenane‐Type Sesquiterpene Dimers Mitigate Lipopolysaccharide‐Induced Inflammation by Inhibiting Toll‐Like Receptor Signaling
Authors: Yang Xue‐Rong , Song Jing‐Ru , Jiang Xiao‐Hua , Dong Fei , Yan Xiao‐Jie , Li Jian‐Xing , Zhao Xue‐Ying , Li Dian‐Peng , Lu Feng‐Lai 
Published: March 2023

AbstractToll‐like receptors (TLRs) recognize pathogen‐associated molecular patterns and trigger an inflammatory response via the myeloid differential factor 88 (MyD88)‐dependent and toll‐interleukin‐1 receptor domain‐containing adapter‐inducing interferon‐β (TRIF)‐dependent pathways. Lindenane type sesquiterpene dimers (LSDs) are characteristic metabolites of plants belonging to the genus Sarcandra (Chloranthaceae). The aim of this study was to evaluate the potential anti‐inflammatory effects of the LSDs shizukaol D (1) and sarcandrolide E (2) on lipopolysaccharides (LPS)‐stimulated RAW264.7 macrophages in vitro, and explore the underlying mechanisms. Both LSDs neutralized the LPS‐induced morphological changes and production of nitric oxide (NO), as determined by CCK‐8 assay and Griess assay, respectively. Furthermore, shizukaol D (1) and sarcandrolide E (2) downregulated interferon β (IFNβ), tumor necrosis factor α (TNFα) and interleukin‐1β (IL‐1β) mRNA levels as measured by reverse transcription polymerase chain reaction (RT‐PCR), and inhibited the phosphorylation of nuclear factor kappa B p65 (p65), nuclear factor kappa‐Bα (IκBα), Jun N‐terminal kinase (JNK), extracellular regulated kinase (ERK), mitogen‐activated protein kinase p38 (p38), MyD88, IL‐1RI‐associated protein kinase 1 (IRAK1), and transforming growth factor‐β‐activated kinase 1 (TAK1) proteins in the Western blotting assay. In conclusion, LSDs can alleviate the inflammatory response by inhibiting the TLR/MyD88 signalling pathway.
Biodiesel Production by Heterogeneous Catalysis and Eco‐friendly Routes
Authors: Welter Rosilene Andrea , Santana Harrson Silva , Gaziola de la Torre Lucimara , Barnes Mark C. , Taranto Osvaldir Pereira , Oelgemöller Michael 
Published: March 2023

AbstractBiodiesel is produced on a large scale as an eco‐friendly substitute and additive to fossil fuels. Catalytic homogeneous processes using strong acids, alkalis, and natural oils have been realized in industry. However, these traditional methods have several disadvantages, such as the generation of large volumes of waste, high water and reagent needs, use of hazardous reagents, high operation costs, and utilization of valuable feedstocks and catalysis, respectively. Different solutions have subsequently been investigated, such as cheap alternative feedstocks, co‐solvents and catalysts, sustainable operational conditions, advanced reactor designs and scales, and advantageous pre‐ and post‐reaction treatments. This review explores and analyzes the main aspects of current biodiesel technologies and opportunities. It also describes some advanced improvement strategies.