bluuebunny/crossref_metadata_2025_split huggingface dataset:
Mitochondrial calcium uniporter deficiency in dentate granule cells remodels neuronal metabolism and impairs reversal learning
Authors: Rose Hadyn M. , Ferrán Beatriz , Ranjit Rojina , Masingale Anthony M. , Owen Daniel B. , Hussong Stacy , Kinter Michael T. , Galvan Veronica , Logan Sreemathi , Díaz‐García Carlos Manlio 
Published: July 2023

AbstractThe mitochondrial calcium uniporter (MCU) is the main route of calcium (Ca2+) entry into neuronal mitochondria. This channel has been linked to mitochondrial Ca2+ overload and cell death under neurotoxic conditions, but its physiologic roles for normal brain function remain poorly understood. Despite high expression of MCU in excitatory hippocampal neurons, it is unknown whether this channel is required for learning and memory. Here, we genetically down‐regulated the Mcu gene in dentate granule cells (DGCs) of the hippocampus and found that this manipulation increases the overall respiratory activity of mitochondrial complexes I and II, augmenting the generation of reactive oxygen species in the context of impaired electron transport chain. The metabolic remodeling of MCU‐deficient neurons also involved changes in the expression of enzymes that participate in glycolysis and the regulation of the tricarboxylic acid cycle, as well as the cellular antioxidant defenses. We found that MCU deficiency in DGCs does not change circadian rhythms, spontaneous exploratory behavior, or cognitive function in middle‐aged mice (11–13 months old), when assessed with a food‐motivated working memory test with three choices. DGC‐targeted down‐regulation of MCU significantly impairs reversal learning assessed with an 8‐arm radial arm water maze but does not affect their ability to learn the task for the first time. Our results indicate that neuronal MCU plays an important physiologic role in memory formation and may be a potential therapeutic target to develop interventions aimed at improving cognitive function in aging, neurodegenerative diseases, and brain injury.image
Primary care in Northern Ontario for transgender people in the context of the COVID‐19 pandemic: A qualitative secondary analysis
Authors: Ziegler Erin , Carroll Benjamin 
Published: July 2023

AbstractPurposeTo examine changes to primary care practice for transgender clients resulting from government mandated public health measures in response to COVID‐19 in Northern Ontario.DesignSecondary analysis of qualitative data using interview transcripts from a dataset that included 15 interviews conducted between October 2020 and April 2021.MethodsThe dataset came from a convergent mixed method study exploring the delivery of primary care services to transgender individuals in Northern Ontario. Qualitative interviews with primary care practitioners including nurse practitioners, nurses, physicians, social workers, psychotherapists, and pharmacists providing care for transgender people in Northern Ontario were included in the secondary analysis.ResultsFifteen primary care practitioner providing care to transgender individuals in Northern Ontario participated in the parent study. Practitioners described their understanding of the effect of the early stages of the COVID‐19 pandemic changes on their practice and the care experience for their transgender patients. Two themes were identified and described by participants: (1) a change in the delivery of care; and (2) barriers and facilitators to care.ConclusionsPractitioners' primary care experiences in the early waves of COVID suggest the integral use of telehealth in Northern Ontario transgender care. Nurses working in advance practice and nurse practitioners are essential in providing continuity of care for their transgender clients.Clinical relevance/SignificanceIdentification of initial practice changes for the primary care of trans people will illuminate avenues for further research. The urban, rural, and remote practice settings in Northern Ontario provide an opportunity for increasing access for gender diverse people in these areas and for developing increased understanding of uptake of telemedicine practice. Nurses are integral to primary care for transgender patients in Northern Ontario.
Safety and efficacy of human platelet extract in skin recovery after fractional CO2 laser resurfacing of the face: A randomized, controlled, evaluator‐blinded pilot study
Authors: Dayan Steven , Gandhi Nimit , Wilson John , Kola Eljona , Jankov Laura Eaton , Copeland Karen , Paradise Chris , Behfar Atta 
Published: July 2023

AbstractBackgroundFractional carbon dioxide (CO2) laser resurfacing is used successfully for facial rejuvenation. Post procedure skincare is a variable that influences downtime caused by pain/tenderness, erythema, crusting, and bruising.AimsThe primary objective of this pilot study was to demonstrate the benefits of human platelet extract (HPE) (plated)™ CALM Serum, a new topical cosmetic product, following fractionated CO2 ablative laser resurfacing treatment to the entire face versus standard of care.MethodsIn a single‐center, randomized, evaluator‐blinded pilot study, a total of 18 subjects were randomized into two groups, CO2 facial resurfacing followed by post‐procedural standard of care (Stratacel silicone gel) or CO2 facial resurfacing with the addition of HPE renewosomes in the CALM Serum.ResultsCALM Serum demonstrated statistically significant less crusting at Day 10 compared to the control group (p = 0.0193) with less downtime in the first 14 days (p = 0.03). Subjects treated with CALM Serum had statistically significant brighter appearing skin at 14 days (p = 0.007) and more youthful looking skin on Days 14 and 30 (p = 0.003 and 0.04, respectively).ConclusionsThis study demonstrates that Renewosome™ technology provides statistically significant post‐laser clinical recovery over silicone gel for reducing crusting, and downtime. Subjects reported less diary days of symptoms of pain/tenderness, redness, crusting/flaking, bruising, and itching in the first 14 days compared to the control group. CALM also demonstrated statistically significant improvements in brighter and more youthful appearing skin. CALM is safe and well tolerated.
Chlamydomonas: Fast tracking from genomics
Authors: Findinier Justin , Grossman Arthur R. 
Published: July 2023

AbstractElucidating biological processes has relied on the establishment of model organisms, many of which offer advantageous features such as rapid axenic growth, extensive knowledge of their physiological features and gene content, and the ease with which they can be genetically manipulated. The unicellular green alga Chlamydomonas reinhardtii has been an exemplary model that has enabled many scientific breakthroughs over the decades, especially in the fields of photosynthesis, cilia function and biogenesis, and the acclimation of photosynthetic organisms to their environment. Here, we discuss recent molecular/technological advances that have been applied to C. reinhardtii and how they have further fostered its development as a “flagship” algal system. We also explore the future promise of this alga in leveraging advances in the fields of genomics, proteomics, imaging, and synthetic biology for addressing critical future biological issues.
Sleep‐related rhythmic movement disorder in adults – A systematic review with a case report
Authors: Michalek‐Zrabkowska Monika , Wieckiewicz Mieszko , Wichniak Adam , Jenca Andrej , Jencova Janka , Frosztega Weronika , Wieczorek Tomasz , Chojdak‐Lukasiewicz Justyna , Sluzewska‐Niedzwiedz Monika , Wojakowska Anna , Poreba Rafal , Mazur Grzegorz , Martynowicz Helena 
Published: July 2023

SummarySleep‐related rhythmic movement disorder is characterised by stereotyped and repetitive rhythmic movements involving large muscle groups during sleep with frequencies between 0.5 and 2 Hz. Most of the published studies on sleep‐related rhythmic movement disorder have focussed on children. Therefore, we performed a systematic review on this topic focussing on the adult population. The review is followed by a case report. The review was conducted in accordance with the Preferred Reporting Items for Systematic Reviews and Meta‐Analyses 2020 guidelines. A total of seven manuscripts (n = 32 individuals) were included in the review. The clinical manifestation of body or head rolling predominated in the majority of included cases (53.13% and 43.75%, respectively). In n = 11 (34.37%) cases, a combination of rhythmic movements was observed. The literature review also revealed a wide spectrum of co‐morbidities: insomnia, restless leg syndrome, obstructive sleep apnea, ischaemic stroke, epilepsy, hypertension, alcohol and drug dependency, mild depression, and diabetes mellitus. The case report presented a 33‐year‐old female who was referred to the sleep laboratory due to a suspicion of sleep bruxism and obstructive sleep apnea. Although the patient was initially suspected of having obstructive sleep apnea and sleep bruxism, after conducting video‐polysomnography she met the criteria for sleep‐related rhythmic movement disorder as she presented body rolling, which were surprisingly most evident during the rapid eye movement sleep stage. In summary, the prevalence of sleep‐related rhythmic movement disorder among adults has not been determined yet. The present review and case report is a good starting point for discussion regarding rhythmic movement disorder in adults and further research on this topic.
Greater sleep variability is associated with higher systemic inflammation in type 2 diabetes
Authors: Reutrakul Sirimon , McAnany J. Jason , Park Jason C. , Chau Felix Y. , Danielson Kirstie K. , Prasad Bharati , Pannain Silvana , Hanlon Erin C. 
Published: July 2023

SummarySleep irregularity and variability have been shown to be detrimental to cardiometabolic health. The present pilot study explored if higher day‐to‐day sleep irregularity and variability were associated with systemic inflammation, as assessed by high‐sensitivity C‐reactive protein, in type 2 diabetes. Thirty‐five patients with type 2 diabetes (mean age 54.3 years, 54.3% female) who were not shift‐workers participated. The presence of diabetic retinopathy was determined. The standard deviation of sleep duration and sleep midpoint across all recorded nights were used to quantify sleep variability and regularity, respectively, assessed by 14‐day actigraphy. The presence and severity of sleep apnea were assessed using an overnight home monitor. Low‐density lipoprotein, haemoglobin A1C and high‐sensitivity C‐reactive protein were collected. Multiple regression analysis using natural‐log‐transformed values was performed to establish an independent association between sleep variability and high‐sensitivity C‐reactive protein. Twenty‐two (62.9%) patients had diabetic retinopathy. The median (interquartile range) of high‐sensitivity C‐reactive protein was 2.4 (1.4, 4.6) mg L−1. Higher sleep variability was significantly associated with higher high‐sensitivity C‐reactive protein (r = 0.342, p = 0.044), as was haemoglobin A1C (r = 0.431, p = 0.010) and low‐density lipoprotein (r = 0.379, p = 0.025), but not sleep regularity, sleep apnea severity or diabetic retinopathy. Multiple regression analysis showed that higher sleep variability (B = 0.907, p = 0.038) and higher HbA1c (B = 1.519, p = 0.035), but not low‐density lipoprotein, contributed to higher high‐sensitivity C‐reactive protein. In conclusion, higher sleep variability in patients with type 2 diabetes who were not shift‐workers was independently associated with higher systemic inflammation, conferring increased cardiovascular risk. Whether sleep interventions to reduce sleep variability can reduce systemic inflammation and improve cardiometabolic health should be investigated.
Relative expression of key genes involved in nucleic acids methylation in Anopheles gambiae sensu stricto
Authors: Djihinto Oswald Y. , Meacci Dario , Medjigodo Adandé A. , Bernardini Federica , Djogbénou Luc S. 
Published: July 2023

AbstractIn vertebrates, enzymes responsible for DNA methylation, one of the epigenetic mechanisms, are encoded by genes falling into the cytosine methyltransferases genes family (Dnmt1, Dnmt3a,b and Dnmt3L). However, in Diptera, only the methyltransferase Dnmt2 was found, suggesting that DNA methylation might act differently for species in this order. Moreover, genes involved in epigenetic dynamics, such as Ten‐eleven Translocation dioxygenases (TET) and Methyl‐CpG‐binding domain (MBDs), present in vertebrates, might play a role in insects. This work aimed at investigating nucleic acids methylation in the malaria vector Anopheles gambiae (Diptera: Culicidae) by analysing the expression of Dnmt2, TET2 and MBDs genes using quantitative real‐time polymerase chain reaction (qRT‐PCR) at pre‐immature stages and in reproductive tissues of adult mosquitoes. In addition, the effect of two DNA methylation inhibitors on larval survival was evaluated. The qPCR results showed an overall low expression of Dnmt2 at all developmental stages and in adult reproductive tissues. In contrast, MBD and TET2 showed an overall higher expression. In adult mosquito reproductive tissues, the expression level of the three genes in males' testes was significantly higher than that in females' ovaries. The chemical treatments did not affect larval survival. The findings suggest that mechanisms other than DNA methylation underlie epigenetic regulation in An. gambiae.
Experiencing gloomy Dis: Tombs, tunnels and the phenomenology of the Roman Underworld in the Phlegraean Fields
Authors: Penn Tim 
Published: July 2023

SummaryRecent work in landscape archaeology has emphasized the importance of considering the experience of moving through landscapes and examining the place of burials within wider landscape contexts. This work recognizes that burial placement was often intended to create and curate experiences and meaning. While burials near roads and waterways have been discussed at length, burials near tunnels, which are an important feature of the road network of Roman central Italy, have not yet featured in discussions of the experience of Roman landscapes or the visibility of graves. This article explores these twin themes in the Phlegraean Fields west of Naples, where burials appear next to the entrances of two monumental tunnels. This placement appears to make an experiential play on the perceptions of the descent into the Underworld as described in Classical literature.
Designing dementia care activation program for Filipino American caregivers
Authors: Ramos Mary Dioise , Heath Jarrett , Lee Danielle , Wright LaNita S. 
Published: July 2023

AbstractObjectiveThis research study aims to identify the fundamental components of community adaptation for a culturally tailored care partner activation program for Filipino American family caregivers of patients with Alzheimer's Disease and related dementia (ADRD).DesignThe study utilized focus group interviews with community nurse leaders, stakeholders, and family caregivers of patients with ADRD.ResultsThe research revealed that education and knowledge about the disease, community‐based services and facilities, support groups and resources, spiritual and cultural values, and access to transportation are all critical components for community adaptation.ConclusionThe findings suggest that a culturally tailored care partner activation program that incorporates these components can enhance the quality of life for Filipino American caregivers and their loved ones with ADRD. The nursing implications of the study underscore the importance of nurses being culturally competent and sensitive to the unique challenges faced by Filipino American caregivers. Nurses can provide valuable support to caregivers by educating them, connecting them with community resources, and advocating for culturally responsive care practices.
Multiperiod channel coordination in franchise networks: The necessity of internal inventory trading and franchiser involvement
Authors: Li Rong , Yang Liangbin , Yue Xiaohang 
Published: July 2023

Facing consumers' growing demand for fast order fulfillment, it is important yet challenging for franchise companies to best incentivize frequent inventory sharing among their independent franchise retailers or dealers to achieve channel coordination. Past literature has only addressed such channel coordination for one‐time inventory sharing using contractual agreements, which unfortunately do not work for frequent inventory sharing. In this study, we consider multiple inventory‐sharing opportunities and propose a novel coordinating mechanism: internal inventory trading, enabled by disruptive IT platforms, such as the OneView platform's Inventory Management module (OVIM). On the OVIM, designed and operated by a brand (franchiser), every retailer (franchisee) can frequently access to all brand inventories virtually as they trade (buy or sell) inventory with one another and the brand. Using dynamic multiperiod games, we investigate how the brand should craft the trade rules and how the retailers should respond and trade periodically in equilibrium. We prove that channel coordination can be achieved in equilibrium, and the coordination requires both an internal inventory‐trading platform and the brand's proactive involvement in trading as the rule and market maker. Specifically, the coordinating trade rules require the brand to (1) only profit from the royalties (not from trading), (2) set the coordinating trade prices (CTPs) to respond to the real‐time channel inventory, and (3) let the buyer and the seller split shipping costs in any proportion, but not offering any subsidy. We provide a detailed characterization of the CTPs for each period, serving multiple purposes beyond simply removing double marginalization. When the channel inventory is high and imbalanced, the CTPs must include a trading reward to prevent retailers from strategically holding more or less inventory than the channel‐optimal amount to “manipulate” future trade prices. The good news is that these CTPs are intuitive (constant or market‐clearing) and can be automated into the trading platform ex ante. These actionable insights can guide franchise companies toward best utilizing their channel inventory and improving their profit and customer satisfaction.
The effect of secukinumab treatment for psoriasis on serum cytokines and correlation with disease severity
Authors: Wu Lan‐Tu‐Ya , Qiao Zhu‐Hui , Tian Jia‐Bin , Lin Jia‐Lin , Hou Su‐Chun , Liu Xiao‐Ming 
Published: July 2023

AbstractObjectiveTo investigate the effects of secukinumab treatment for psoriasis on different functional cytokines and inflammatory mediators in patients’ serumMethodsEnzyme‐linked immunosorbent assay was used to detect interleukin (IL)‐1β and IL‐1RA associated with intrinsic immunity; IL‐6, IL‐18, and growth regulated oncogene alpha (GROα) associated with neutrophils; IL‐12, tumour necrosis factor (TNF)‐α, and interferon (IFN)‐γ associated with Th1; IL‐23, IL‐17A, and IL‐22 associated with Th17; Thymus activation regulated chemokine (TARC), IL‐13, and defensin beta 2 (DEFB2) associated with Th2; Vascular endothelial growth factor (VEGF)‐A and IL‐10 associated with angiogenesis; and IFN‐γ associated with sepsis in the peripheral blood of 12 patients with common psoriasis treated with secukinumab and 15 healthy controls. IL‐23, IL‐17A, IL‐22 associated with Th17; TARC, IL‐13, DEFB2 associated with Th2; VEGF‐A, IL‐10 associated with angiogenesis and procalcitonin (PCT) associated with sepsis. The differences in expression of the above cytokines before and after treatment and the correlation with psoriasis disease severity［Psoriasis Area Severity Index(PASI) score］, age, and disease duration were analyzed.ResultsThe mean PASI score of the enrolled patients with moderate to severe psoriasis was 21.6 ± 11.0 before treatment and decreased to below 1 after treatment. Serum IL‐6; IL‐18, GROα, IFN‐γ, TNF‐α, VEGF‐A, and IL‐17A were significantly higher than normal. And IL‐17A and IFN‐γ were positively correlated with disease duration and age, and IL‐18 was positively correlated with PASI score. The expression levels of IL‐6, GROα, VEGF‐A, IFN‐γ, TNF‐α, IL‐17A and IL‐23 were significantly lower after secukinumab treatment compared with those before treatment, but the expression levels of IFN‐γ, VEGF‐A, TARC, IL‐13, and DEFB2 were still significantly higher than those of normal subjects after treatmentConclusionssecukinumab clears skin lesions by antagonizing IL‐17A and simultaneously decreasing the expression levels of IL‐6, GRO α, VEGF‐A, IFN‐γ, TNF‐α, IL‐17A, and IL‐23.
Biochar combined with magnesium fertilizer improves cabbage (Brassica oleraceae L.) yield by modulating physicochemical characteristics and the bacterial community in acidic soil
Authors: Li Hongzhao , Yan Tongbo , Fu Tianhong , Hao Yongzhou , Li Jing , Tan Yi , Li Zhen , Peng Yutao , Chen Xiao , Chang Jingjing , Song Zhao , Yu Min , Li Xuewen , Li Yalin , Song Shiwei , Zhang Baige 
Published: July 2023

AbstractBiochar and magnesium (Mg) fertilizers have gained increasing attention in agriculture for their potential benefits. However, their combined effects on vegetable growth in southern acidic soils have not been fully elucidated. To address this issue, a study was conducted with six treatments all of which included a baseline amount of compound fertilizer (CK), an increased amount of compound fertilizer (F), Mg fertilizer (M), biochar (BC), biochar combined with Mg (BCM) and an increased amount of compound F with Mg (FM), to investigate their synergistic effects on soil physicochemical properties, enzyme activity, microorganisms and cabbage yield. The results of the study showed that compared with CK, the M, BC and BCM treatments all increased soil organic matter (SOM), exchangeable magnesium (Ex‐Mg), acid phosphatase (ACP) and yield, while the F treatment led to a 5% decrease in SOM. The BCM treatment significantly increased SOM, Ex‐Mg, ACP by 14%, 40%, and 25%, respectively. The most abundant phyla in BCM processing were Proteobacteria (21%), Chloroflexi (19%) and Actinomycetes (17%); the most abundant genera were Bacillus (8%). Further, the effect of BCM on crop growth promotion was better than that of FM, and the economic analysis showed that compared with FM, the benefit of BCM increased by 14%. Through structural equation modelling analysis, the synergistic increase in yield was because of the increase in soil TP, Ex‐Mg, NO3−–N and β‐GC caused by BCM, improving soil nutrients and microbial communities, thereby promoting cabbage growth. Therefore, the combined application of biochar and Mg in acidic soil could significantly improve soil fertility and crop productivity, while also reducing the need for costly chemical fertilizers, thereby offering a promising and cost‐effective approach for sustainable agriculture.
The DEAD‐box helicase RCF1 plays roles in miRNA biogenesis and RNA splicing in Arabidopsis
Authors: Xu Chi , Zhang Zhanhui , He Juan , Bai Yongsheng , Cui Jie , Liu Lin , Tang Jihua , Tang Guiliang , Chen Xuemei , Mo Beixin 
Published: July 2023

SUMMARYRCF1 is a highly conserved DEAD‐box RNA helicase found in yeast, plants, and mammals. Studies about the functions of RCF1 in plants are limited. Here, we uncovered the functions of RCF1 in Arabidopsis thaliana as a player in pri‐miRNA processing and splicing, as well as in pre‐mRNA splicing. A mutant with miRNA biogenesis defects was isolated, and the defect was traced to a recessive point mutation in RCF1 (rcf1‐4). We show that RCF1 promotes D‐body formation and facilitates the interaction between pri‐miRNAs and HYL1. Finally, we show that intron‐containing pri‐miRNAs and pre‐mRNAs exhibit a global splicing defect in rcf1‐4. Together, this work uncovers roles for RCF1 in miRNA biogenesis and RNA splicing in Arabidopsis.