bluuebunny/crossref_metadata_2025_split huggingface dataset:
Clustering climate and management practices to define environmental challenges affecting gastrointestinal parasitism in Katahdin sheep
Authors: Arisman Brian C , Burke Joan M , Morgan James L M , Lewis Ronald M 
Published: January 2023

Abstract
Gastrointestinal nematodes (GIN) negatively affect the performance and well-being of sheep. Due to anthelmintic resistance, GIN are difficult to control leading producers to choose breeds that can exhibit resistance to parasitism. An example is Katahdin sheep. Katahdins are raised in various climates and management systems in the United States. These environmental factors can be combined to form eco-management groupings or clusters. We hypothesized that GIN challenge varies predictably based on the characteristics of these environmental clusters. Forty Katahdin producers from across the United States were surveyed for management information, with body weights (BW), fecal egg counts (FEC), and FAMACHA scores (FAM) available from 17 of the 40 flocks. The performance data included 3,426 lambs evaluated around 90 d of age. Management and climate data were combined into clusters using multiple correspondence and principal component (PC) analysis. Performance data were aligned with their corresponding cluster. Depending on the trait, eco-management cluster, birth-rearing type, sex, and, as a covariate, dam age, were fitted as systematic effects with ANOVA. Clusters also were formed based on climate or management data alone. When compared with fitting the eco-management clusters, they defined less variation in each of the traits based on Akaike and Bayesian information criterion, and adjusted r2 values. To further examine variation defined by eco-management clusters, residuals from an ANOVA model excluding eco-management cluster were retained, and their correlation with PC loadings calculated. All PC loadings were included as potential independent variables and tested for significance using backward stepwise regression. The PC loadings with a correlation |≥0.49| explained significant variation in each trait and were included in the final models chosen; adjusted r2 values for BW, FEC, and FAM were 0.90, 0.81, and 0.97, respectively. When analyzing GIN challenge, eco-management clusters corresponding with hotter temperatures and greater rainfall, and with pasture-born lambs, suffered greater parasitism. Conversely, the eco-management clusters with lambs turned out to pasture at older ages benefited from reduced parasitism. Through the formation of eco-management clusters, an environmental variable can be defined to study interactions of genotypes to their environment, providing a potentially useful tool for identifying parasite-resistant sheep.
Signatures of selection in indigenous Chinese cattle genomes reveal adaptive genes and genetic variations to cold climate
Authors: Huang Ning , Zhao Lihong , Wang Jinpeng , Jiang Qiang , Ju Zhihua , Wang Xiuge , Yang Chunhong , Gao Yaping , Wei Xiaochao , Zhang Yaran , Xiao Yao , Liu Wenhao , Lu Shaoxiong , Huang Jinming 
Published: January 2023

AbstractCold climate shapes the genome of animals and drives them to carry sufficient genetic variations to adapt to changes in temperature. However, limited information is available about the genome-wide pattern of adaptations to cold environments in cattle. In the present study, we used 777K SNP genotyping (15 Chinese cattle breeds, 198 individuals) and whole genome resequencing data (54 cattle breeds of the world, 432 individuals) to disentangle divergent selection signatures, especially between the cold-adapted (annual average temperature of habitat, 6.24 °C to 10.3 °C) and heat-adapted (20.2 °C to 24.73 °C) Chinese native cattle breeds. Genomic analyses revealed a set of candidate genes (e.g., UQCR11, DNAJC18, EGR1, and STING1) were functionally associated with thermogenesis and energy metabolism. We also characterized the adaptive loci of cattle exposed to cold temperatures. Our study finds new candidate genes and provides new insights into adaptations to cold climates in cattle.
Effects of a milk oligosaccharide biosimilar on fecal characteristics, microbiota, and bile acid, calprotectin, and immunoglobulin concentrations of healthy adult dogs treated with metronidazole
Authors: Belchik Sara E , Oba Patricia M , Wyss Romain , Asare Paul T , Vidal Sara , Miao Yong , Adesokan Yemi , Suchodolski Jan S , Swanson Kelly S 
Published: January 2023

Abstract
In recent dog and cat experiments, a novel milk oligosaccharide biosimilar (GNU100) positively modulated fecal microbiota and metabolite profiles, suggesting benefits to gastrointestinal health. The objective of this study was to investigate the effects of GNU100 on the fecal characteristics, microbiota, and bile acid (BA) concentrations of healthy adult dogs treated with antibiotics. Twelve healthy adult female dogs (mean age: 3.74 ± 2.4 yr) were used in an 8-wk crossover design study (dogs underwent both treatments). All dogs were fed a control diet during a 2-wk baseline, then randomly allotted to 1 of 2 treatments (diet only or diet + 1% GNU100) for another 6 wk. From weeks 2 to 4, dogs were orally administered metronidazole (20 mg/kg BW) twice daily. Fecal scores were recorded daily and fresh fecal samples were collected at weeks 2, 4, 5, 6, and 8 for measurement of pH, dry matter, microbiota populations, and BA, immunoglobulin A, and calprotectin concentrations. On weeks 0, 4, and 8, blood samples were collected for serum chemistry and hematology analysis. All data were analyzed as repeated measures using the Mixed Models procedure of SAS version 9.4, with significance considered P &lt; 0.05. Metronidazole increased (P &lt; 0.0001) fecal scores (looser stools) and modified (P &lt; 0.05) fecal microbiota and BA profiles. Using qPCR, metronidazole reduced fecal Blautia, Fusobacterium, Turicibacter, Clostridium hiranonis, and Faecalibacterium abundances, and increased fecal Streptococcus and Escherichia coli abundances. DNA sequencing analysis demonstrated that metronidazole reduced microbial alpha diversity and influenced the relative abundance of 20 bacterial genera and families. Metronidazole also increased primary BA and reduced secondary BA concentrations. Most antibiotic-induced changes returned to baseline by week 8. Fecal scores were more stable (P = 0.01) in GNU100-fed dogs than controls after antibiotic administration. GNU100 also influenced fecal microbiota and BA profiles, reducing (P &lt; 0.05) the influence of metronidazole on microbial alpha diversity and returning some fecal microbiota and secondary BA to baseline levels at a quicker (P &lt; 0.05) rate than controls. In conclusion, our results suggest that GNU100 supplementation provides benefits to dogs treated with antibiotics, providing more stable fecal scores, maintaining microbial diversity, and allowing for quicker recovery of microbiota and secondary BA profiles which play an essential role in gut health.
Technical note: simultaneous determination of amino thiols in pig tissue by ultra-high performance liquid chromatography with fluorescence detection
Authors: Rudar Marko , Gachman Alexandra , Boersma Melissa 
Published: January 2023

AbstractSulfur amino acid nutrition and metabolism are linked to animal disease. While validated methods for the determination of amino thiol levels in plasma or serum are available, there is a dearth of validated methods for their measurement in tissue. A robust and reproducible ultra-high performance liquid chromatography method has been validated for the simultaneous determination of concentrations of cysteine (Cys), cysteinylglycine (CysGly), homocysteine (Hcys), γ-glutamylcysteine (γ-GluCys), and glutathione (GSH) in pig tissue. Tissue was homogenized and deproteinized with trichloroacetic acid. Amino thiols in the acid-soluble fraction of the tissue homogenate were reduced with tris-(2-carboxyethyl)-phosphine hydrochloride and derivatized with 4-(aminosulfonyl)-7-fluoro-2,1,3-benzoxadiazole (ABD-F). Amino thiols were resolved under reversed-phase gradient conditions on a Waters Acquity BEH C18 column (1.7 µm, 2.1 mm × 100 mm) within 4.5 min and detected with fluorescence. The peak area ratio of analyte to 2-mercaptopropionylglycine internal standard, added to external calibration standards and samples, was used to develop linear calibration curves. Linear calibrations were performed over the range of 15–1,500 nmol/g for Cys, CysGly, Hcys, and γ-GluCys and 150–15,000 nmol/g for GSH. Linearity, lower limit of detection, lower limit of quantitation, accuracy, precision, sample stability, and carryover were evaluated. We demonstrate excellent linearity for all analytes within their respective concentration range (r2 &gt; 0.99) and excellent recovery of amino thiols from spiked samples (mean ± SD across tissues; Cys, 100.0 ± 2.2%; CysGly, 95.4 ± 5.1%; Hcys, 96.6 ± 2.0%; γ-GluCys, 102.2 ± 2.7%; and GSH, 100.6 ± 3.3%). The intra-day and inter-day precisions did not exceed 5% and 10%, respectively. Repeated freezing and thawing of tissue homogenate did not affect measured amino thiol concentrations, ABD-labeled amino thiols were stable for 1 wk after derivatization, and there was no sample carryover across consecutive injections. We confirm the identity of each ABD-labeled amino thiol with Orbitrap mass spectrometry. Finally, we apply the method to the determination of amino thiol concentrations in liver and jejunum tissues in newly weaned pigs and show that despite elevated Cys and maintained GSH concentrations in liver, both γ-GluCys and GSH decline in jejunum of weaned pigs.
Formation of hierarchical assemblies by collagen peptides derived from fish skin and bladder and their subsequent application as antiperoxide agents in lipid-rich food
Authors: Sumeet Charitha , Bajaj Mayur , Kumar Indresh , Yelleti Geethika , Asokan Vishwadeep , Tagadghar Pawan , Banerjee Pradipta 
Published: January 2023

AbstractThis study attempts to identify the significant role played by the secondary and tertiary structure of collagen-derived peptides that are involved in lipid peroxide quenching in food products. Fish collagen hydrolysate (CH) was extracted with an efficiency of 70%. The constituent peptides of CH (8.2–9.7 kDa) existed in a polyproline-II (PP-II) conformation and at a minimum concentration of 1 mg ml−1 and pH range 7 to 8, assembled into a stable, hierarchical, quasi-fibrillar (QF) network. The peroxide quenching activity of this QF-CH increased with increasing ionic stability of the assembly and decreased upon proteolytic dismantling. Upon being used as an additive, the QF-CH reduced peroxide formation by 84.5% to 98.9% in both plant and fish-based oil and increased the shelf life of soya oil by a factor of 5 after 6 months of storage. The addition of QF-CH to cultured cells quenched peroxide ions generated in situ and decreased stressor activity by a factor of 12.16 abundant peptides were identified from the CH. The reason behind the high efficacy displayed by CH was attributed to its unique charge distribution, prevalence of proton-donating amino acid residues and proximal charge delocalization by the QF network, making fish derived CH a suitable substitute for antiperoxide agents in lipid-rich food.
Does A History of Malignancy Lead to Worse Outcomes in a Single-center Burn Unit?
Authors: Sljivic Sanja , Nam Jason , Matthews Robert , Agala Chris B , Hollowell Jamie , Nizamani Rabia , King Booker , Williams Felicia N 
Published: January 2023

AbstractA history of malignancy is associated with worse outcomes in cardiac disease and trauma. Our objective was to determine if a past medical history or comorbid condition of cancer portends an increased morbidity or mortality in burns or skin-sloughing disorders at our institution. Patients were identified using our Institutional Burn Center registry and linked to the clinical and administrative data. All patients admitted between January 1, 2014 and June 30, 2021 were eligible for inclusion. Demographics, length of stay, comorbid conditions and mortality were evaluated. Statistical analysis was performed with Kruskal–Wallis, chi-square, and Fisher’s exact tests. Seven thousand three hundred seventy-two patients were admitted during this time period. Three hundred eighty-six patients had a history of cancer (5%). Patients with a history of cancer were older (56 vs 44 years, P &lt; .0001). They had a significantly longer length of stay (16 vs 10 days, P &lt; .0001). They also had larger burns and higher hospital costs ($147,021 versus $83,788, P &lt; .0001), were more likely to be male and more likely to have a skin-sloughing disorder. A history of cancer was not associated with increased odds of burn mortality. Thus, a history of cancer is associated with increased lengths of stay and costs in patients admitted for burn injury or skin-sloughing disorders, but not associated with increased mortality. Further study is warranted to investigate and mitigate what aspects of their care could be adjusted to improve outcomes.
Mixed and Purely Hyperechoic Breast Masses: A Radiologic-Pathologic Review
Authors: Chesebro Allyson L , Joseph Sumita , Kuba M Gabriela , Lester Susan , Giess Catherine S 
Published: January 2023

AbstractBreast US is a mainstay of modern-day breast imaging, especially in the diagnostic and interventional realm. The BI-RADS atlas described six echo patterns relative to the subcutaneous mammary fat: anechoic, hypoechoic, complex cystic and solid, isoechoic, heterogeneous, and hyperechoic. Hyperechoic breast masses demonstrate increased echogenicity relative to subcutaneous mammary fat or equal to fibroglandular tissue. Pathologically, the hyperechoic pattern at breast US results from the intermingling of different components: adipose tissue, fibrous tissue or stroma, secretions, blood or vascularity, and calcifications. Most hyperechoic masses are benign, especially homogeneously hyperechoic masses. However, hyperechogenicity does not exclude malignancy. Two echo patterns have been identified in hyperechoic malignant lesions, including those with a hypoechoic center and hyperechoic rim known as the rim pattern and a mass with hyperechoic areas distributed through the mass known as a dispersed pattern. This article aims to illustrate the echogenic patterns of breast lesions and various benign and malignant hyperechoic breast lesions with radiologic-pathologic correlation and to increase awareness of heterogeneously hyperechoic breast lesions as a manifestation of malignancy.
Increasing seawater temperatures in the Levantine Basin, eastern Mediterranean, may elicit increased activity in slipper lobsters, Scyllarides latus (Latreille, 1803) (Decapoda: Achelata: Scyllaridae)
Authors: Goldstein Jason S , Spanier Ehud 
Published: January 2023

Abstract
Temperature is a predominant motivator for movement and activity over a wide range of mobile marine ectotherms. Water temperature modulates the movements of many lobster species, which can vary widely over spatial and temporal scales. Providing insight into the thermal preferences (and refuges) that some lobsters seek remains a key tenet to our understanding of the behavioral ecology of these animals. The Mediterranean slipper lobster, Scyllarides latus (Latreille, 1803), shows seasonal movements throughout most of its range and is subject to a changing thermal environment. Through a series of laboratory-based trials, we examined the movement activity of S. latus and tested the hypothesis that S. latus engage in increased movements when subjected to temperatures outside their preferred range. To examine this, we exposed a set of lobsters (N = 10, CLavg= 83.1 ± 6.1 cm) to the same thermal profiles as in the field and assayed their locomotion using activity wheels. Our laboratory results showed that individuals of S. latus are more active at higher temperatures during dawn hours and revealed that warming water temperatures elicited markedly longer movements over a similar timeframe. Combined, these findings suggest that increasing water temperatures in the eastern Mediterranean (Levant) may affect lobster movements and could alter seasonal patterns of distribution as well.
Application of reinforcement learning based on curriculum learning for the pipe auto-routing of ships
Authors: Kim Youngsu , Lee Kyungho , Nam Byeongwook , Han Youngsoo 
Published: January 2023

Abstract
The pipe routing of ships has been manually performed by experts, and the design quality depends on the competence of the experts. Therefore, studies on pipe-routing automation and optimization are required. In addition, the pipe-routing task in a ship that requires frequent pipe-routing modifications requires a long time to be optimized. In this study, we developed a methodology that enables a rapid response in situations where frequent pipe-routing modifications are required by applying curriculum learning that can be stably learned by gradually solving easy-to-complex problems. In addition, this study aimed to minimize the length of the pipe and number of bends as an objective function. Finally, the proposed methodology was verified by comparing it with existing studies that used the A*, jump point search, and reinforcement-learning algorithms to determine the search speed, number of bends, and length of the path.
Study on the relationship between machining errors and transmission accuracy of planetary roller screw mechanism using analytical calculations and machine-learning model
Authors: Wu Hanlin , Wei Peitang , Hu Rui , Liu Huaiju , Du Xuesong , Zhou Pengliang , Zhu Caichao 
Published: January 2023

AbstractCorrelation between machining errors and transmission accuracy of planetary roller screw mechanism (PRSM) plays an important role in tolerance design. In this study, analytical calculations, machine learning, and experimental verification are utilized for exploring the internal correlation between the machining errors and the transmission accuracy of the PRSM. A multi-roller meshing transmission error model is established, which comprehensively considers the eccentric error, nominal diameter error, flank angle error, and cumulative pitch error of the screw, roller, and nut. The importance coefficients of various machining errors on the transmission error are determined using the random forest algorithm. A genetic algorithm-back propagation neural network algorithm-based method is utilized for training the dataset generated via analytical calculations. The results show that the proposed analytical calculation model reflects the alternate meshing characteristics of rollers during the PRSM motion, providing a more accurate prediction of the transmission error than the existing prediction methods. For an actual mean travel deviation, the most significant machining error is the cumulative pitch error of the screw, whereas for the actual bandwidth of useful travel, the most significant machining errors are the eccentric errors of the screw and nut. The proposed prediction formulae for transmission error considering the essential machining errors illustrate reasonable prediction accuracy, with an average error of 10.63% for the actual mean travel deviation and 14.27% for the actual bandwidth of useful travel compared with the experiments, which can effectively support the direct design of PRSM tolerance in engineering practice.
Making Medical History Relevant to Medical Students: The First Fifty Years of the Calgary History of Medicine Program and History of Medicine Days Conferences
Authors: Stahnisch Frank W 
Published: January 2023

Abstract
Medical historians and educators have long lamented that the integration of the study of the history of medicine into the educational curricula of medical schools and clinic-based teaching has been protractedly troubled. Employing the development of the history of medicine program at the University of Calgary as a case study, this article emphasizes the importance of integrating medical history with teaching schedules to further students’ insights into changing health care settings, the social contingency of disease concepts, and socio-economic dependences of medical decision-making. History of medicine programs can furnish plentiful opportunities for research training through summer projects, insight courses, and field practica. This article explores the first fifty years of the History of Medicine and Health Care Program in Calgary and considers the impact of interdisciplinary cooperation as well as the role of interprofessional undergraduate and clinical medical education. Through this exploration, I argue that medical history should be a central part of study curricula, that a historical understanding can provide a robust background for physicians in a fast-changing world in the clinic, and that through their disciplinary expertise, medical historians play a fruitful role in scholarly and teaching exchanges with medical students and clinicians in the modern medical humanities.
Ambitious for change? A critical appraisal of the Canadian indicator framework of the sustainable development goals
Authors: Smith-Carrier Tracy , On Jacqueline 
Published: January 2023

Abstract
This article explores the indicators and targets identified in the Canadian Indicator Framework, a localization of the UN Sustainable Development Goals for the country of Canada. Applying a critical theoretical lens and a human rights approach, the authors explore each of the ‘ambitions’ proposed by the federal government and assess their suitability in meeting the magnitude of transformative change that will be necessary to meet the goals of the 2030 Agenda for Sustainable Development. In considering each of the Canadian ambitions proposed to realize Sustainable Development Goals 1 to 17, and the framework as a whole, the authors conclude that a business-as-usual stance has been applied. Many of the Canadian ambitions have ‘no specific target’ identified, offering no baseline measures or concrete standards from which to benchmark and monitor progress. The ones that do are not tremendously transformative, leading to a framework that does not present a dramatic departure from existing policy and practice arrangements. The character of the Canadian ambitions to the Sustainable Development Goals are revealed, not as concrete change strategies, but as mere aspirations, albeit more for the status quo than for transformational action. To translate the Canadian ambitions into actions, human rights must be infused into the Canadian Indicator Framework, and these must be substantive, de facto, rights—rights that people can actually claim, and hold state actors accountable to.
The Use of Trade Coercion and China’s Model of ‘Passive-Aggressive Legalism’
Authors: Czapnik Ben , Mercurio Bryan 
Published: January 2023

ABSTRACT
There is a growing view among both governments and commentators, especially in the West, that China engages in acts of trade coercion which violate the law and spirit of the multilateral trading system. And while there is a developing literature discussing this issue, the debate currently lacks clarity about how China’s approach differs from other types of coercion which do not inspire the same backlash. This article advances the literature by suggesting that Chinese trade coercion is particularly threatening to the rules-based system (RBS) as it uses methods that had largely been eradicated under the GATT/WTO system. Chinese trade coercion therefore represents a “new” and important phenomenon in international trade as it uses coercion for strategic purposes, usually as retaliation for perceived affronts in matters unrelated to trade. We label this approach “passive-aggressive legalism” because, rather than relying on open dialogue to resolve diplomatic frictions, China implements informal and “plausibly deniable” retaliatory measures to indirectly make its displeasure known and felt. The article concludes by making broader claims about why China’s approach represents a threat to the WTO system and may even undermine the effectiveness of rules-based constraints in other domains of global regulation.