bluuebunny/crossref_metadata_2025_split huggingface dataset:
Clinical and Imaging Characteristics of Intraductal Papillary Neoplasms of the Breast: a prospective trial
Authors: Wang Yingjiao , Li Yuechong , Qu Yang , Zhou Yidong , Sun Qiang , Shen Songjie 
Published: January 2023

Abstract
Background
Intraductal papillary neoplasms(IPNs) often have a similar clinical and imaging presentation, making them difficult to diagnose. We designed this study to refine and compare intraductal papillary neoplasms' clinical and imaging characteristics.
Methods
We recruited patients who were diagnosed with IPN and collected their clinical, imaging, and pathological data. We compared the clinical and imaging characteristics of benign, atypical hyperplasia, and malignant lesions. We also compared the diagnostic efficacy of ultrasound and mammography.
Results
The mean age of malignant patients was 57 years old, which was significantly higher than that in the other groups (48 years in the benign group and 47 years in the atypical hyperplasia group). The proportion of patients with malignant lesions clinically presenting as palpable masses (31.3%) was significantly higher than that of benign (8.6%) (P < 0.05). The proportion of malignant lesions presenting in the periphery was 40.6% compared to 22.4% for benign (P < 0.05). In ultrasonography, characteristics that showed statistically significant differences between benign and malignant lesions were the shape of the mass and calcification (P < 0.05). In mammography, differences were found in mass shape, calcification, and density of masses and glands (P < 0.05).
Conclusions
Clinical features such as age, symptoms, lesion location, and imaging characteristics such as shape, calcification, mass, and density may help to differentiate the classifications of IPNs.
Trial registration:
This study was registered at ClinicalTrials.gov on 12/06/2020 (identifier: NCT04429269).
Improved salt-tolerance of transgenic soybean by stable over-expression of AhBADH gene from Atriplex hortensis
Authors: Yu Zhijing , Niu Lu , Cai Qinan , Wei Jia , Shang Lixia , Yang Xiangdong , Ma Rui 
Published: January 2023

Abstract
An effective strategy for increasing the productivity of major crops under salt stress conditions is the development of transgenics that harbor genes responsible for salinity tolerance. Betaine aldehyde dehydrogenase (BADH) is a key enzyme involved in the biosynthesis of the osmoprotectant, glycine betaine (GB), and osmotic balance in plants, and several plants transformed with BADH have shown significant improvements in salt and drought tolerance. However, very few field-tested transgenic cultivars have been reported, as most of the transgenic studies are limited to laboratory or green house experiments. In this study, we demonstrated through field experiments that BADHfrom Atriplex hortensis (AhBADH) confers salt tolerance when transformed into soybean (Glycine max L.). AhBADH was successfully introduced into soybean by Agrobacterium mediated transformation. A total of 256 transgenic plants were obtained, out of which 47 lines showed significant enhancement of salt tolerance compared to non-transgenic control plants. Molecular analyses of the transgenic line TL7 with the highest salt tolerance exhibited stable inheritance and expression of AhBADH in progenies with a single copy insertion. TL7 exhibited stable enhanced salt tolerance and improved agronomic traits when subjected to 300mM NaCl treatment. Currently, the transgenic line TL7 with stable enhanced salt tolerance, which has been cleared for environmental release, is under biosafety assessment. TL7 stably expressing AhBADH could then be applied in commercial breeding experiments in order to genetically improve salt tolerance in soybean.
Association of obesity with incident atrial fibrillation in Korea and the United Kingdom
Authors: Choi Sung Hwa , Yang Pil-Sung , Kim Daehoon , Sung Jung-Hoon , Jang Eunsun , Yu Hee Tae , Kim Tae-Hoon , Pak Hui-Nam , Lee Moon-Hyoung , Lip Gregory Y.H. , Joung Boyoung 
Published: January 2023

Abstract
Background: Obesity has been linked to atrial fibrillation (AF) burden and severity, and epidemiological studies suggest that AF is more prevalent in whites than Asian. We aimed to investigate whether obesity mediates associations with AF in Europe and Asia using patient-level data comparisons of two cohort studies.
Methods: Using Korean National Health Insurance Service’s Health Screening (NHIS-HealS) and U.K. Biobank data, we included 401,206 Korean and 477,926 British aged 40–70 years without previous AF who received check-ups. The incidence and risk of AF were evaluated regarding different body mass index (BMI) values.
Results: The obese proportion (BMI³30.0 kg/m2, 2.8% vs. 24.3%, P<0.001) was higher in the U.K. than the Korean. In the Korean and U.K. cohort, the age- and sex-adjusted incidence rates of AF were 4.97 and 6.54 per 1,000 person-years among obese individuals. Compared to Koreans, the risk of AF was higher in the British population, with adjusted hazard ratios of 1.41 (Korea, 95% CI, 1.26–1.58) and 1.68 (UK, 95% CI, 1.54–1.82) in obese participants (P for interaction<0.05).
Conclusions: Obesity was associated with AF in both populations. British subjects had a greater incidence of AF related to the high proportion of obese individuals, but the risk of AF was also increased when participants into obese categories.
Scale down and optimized automated production of [68Ga]68Ga-DOTA-ECL1i PET tracer targeting CCR2 expression
Authors: Migliari Silvia , Scarlattei Maura , Baldari Giorgio , Ruffini Livia 
Published: January 2023

Abstract
Background: recently it has been identified a short peptide that showed allosteric antagonism against C-C motif chemokine receptor 2 (CCR2) expressed on inflammatory monocyte and macrophages. A 7-D-amino acid peptidic CCR2 inhibitor called extracellular loop 1 inverso (ECL1i), d(LGTFLKC) has been identified and labeled to obtain a new probe for positron emission tomography in pulmonary fibrosis, heart injury, abdominal aortic aneurysm inflammation, atherosclerosis, head and neck cancer.
Our goal was to develop, optimize and validate an automated synthesis method and quality control system for [68Ga]68Ga-DOTA-ECL1i to make it available for a broader community.
The synthesis of [68Ga]68Ga-DOTA-ECL1i was done using the Scintomics GRP® module with the already estabilished synthesis template for [68Ga]68Ga-DOTATOC/[68Ga]68Ga-PSMA. The radiopharmaceutical production was optimized using different amount of DOTA-ECL1i (from 50 μg to 10 μg), evaluating synthesis efficiency and relevant quality control parameters in accordance with the European Pharmacopeia.
Results: best results were yielded with 20 μg DOTA-ECL1i and then the process validation was carried out by producing three different batches on three different days obtaining an optimal radiochemical yield (66,69%) as well as radiochemical purity (100%) and molar activity (45.41 GBq/µmol).
Conclusions: [68Ga]68Ga-DOTA-ECL1i was successfully synthesized and it is, thus, available for multi-dose application in clinical settings.
Structure and mechanism of the K+/H+ exchanger KefC
Authors: Gulati Ashutosh , Kokane Surabhi , Boerema Annemarie , Alleva Claudia , Meier Pascal , Matsuoka Rei , Drew David 
Published: January 2023

Abstract

Intracellular potassium (K+) homeostasis is fundamental to cell viability. In addition to channels, cellular K+ levels are maintained by various ion-transporters. One major family are the proton-driven K+ efflux transporters. In gram-negative bacteria, K+/H+ exchange activity is important for osmoregulation and, in plants, their activity is critical for efficient photosynthesis and growth. Despite their wide-spread importance, the structure and molecular basis for K+-selectivity is poorly understood. Here, we report the cryo-EM structure of the model glutathione (GSH)-gated K+ efflux transporter KefC from E. coli at 3.2 Å resolution. KefC forms a homodimer and is overall similar to the Na+/H+ antiporter NapA captured in an inward-facing conformation. By structural assignment of a coordinated K+ ion, MD simulations and SSM-based electrophysiology, we demonstrate how the ion-binding site in KefC is adapted for binding a dehydrated K+ ion, which contrasts with NapA, which coordinates a partially hydrated Na+ ion. The substrate-K+ ion is completely enclosed within the transport domains, consistent with an elevator alternating-access mechanism. KefC harbours C-terminal RCK regulatory domains, similar to the TrkA domain of K+-channels, forming homodimers that are stabilised by AMP and GSH. In the current KefC structure, a pair of domain-swapped helices in the RCK domains inhibit transport by directly interacting with the ion-transport domains. Taken together, we propose that KefC is activated by detachment of the RCK domains and ion-selectivity exploits the biophysical properties likewise adapted by cation ion-channels.
Bacteria from the Sonoran Desert with nematicidal potential against phytopathogenic nematodes
Authors: Chavarria-Quicaño Estefany , Contreras-Jácquez Victor , Carrillo-Fasio Armando , Torre-González Francisco De la , Asaff-Torres Ali 
Published: January 2023

Abstract
The use of microorganisms and/or their metabolites (e.g., Bacillus, Pseudomonas, etc.) has been proposed as an eco-friendly alternative for controlling phytopathogenic nematodes (PPNs). However, one of the challenges is that microorganisms can thrive in crops affected by climate change, salt stress and drought. Consequently, the search for microorganisms adapted to these conditions has attracted attention. Deserts are characterized by high temperatures, water scarcity, and high salinity, among other harsh conditions; hence, they are a perfect niche for isolating microorganisms adapted to extreme environments. The purpose of this work was to isolate and characterize bacteria from rhizospheric soil samples collected in the Sonoran Desert with the potential for PPN control. Approximately 100 thermotolerant bacteria were isolated, and among them the secretome of the strain identified as Bacillus paralicheniformis TB197 showed the highest nematicidal activity (96%) against Meloidogyne incognita in vitro. Additionally, in field assays, the TB197 secretome effectively suppressed infections caused by M. enterolobii. In greenhouse experiments, TB197 endospores reduced the severity of infection by 81% compared with the control (p≤0.05). Finally, the efficacy of TB197 endospores at the open field level was demonstrated against M. incognita in the tomato crop, where the galling index (GI) was reduced by 84%, and against Radopholus similisin the banana crop, where root necrosis was reduced by 81%, both compared with the controls (p≤0.05). Owing to their efficacy in controlling PNN infections, the endospores and secondary metabolites secreted by the B. paralicheniformis TB197 strain can be used to develop effective bionematicides.
Identification of target genes in recurrent implantation failure women associated with maternal-fetal immune
Authors: Fan Meiling , Liu WeiNa , Zhang Ning , Jin Xianglan , Wang Hui 
Published: January 2023

Abstract

Background Recurrent implantation failure (RIF) is one of the main reasons for the unsatisfactory pregnancy rate of in vitro fertilization-embryo transfer, however the underlying mechanism is still obscure. Methods An online tool (Gene Expression Omnibus 2R) was used to identify gene expression differences in RIF women and fertility controls endometrium. Publicly available databases including GeneMANIA, Predicting Associated Transcription factors from Annotated Affinities, JASPAR and Protein Analysis Through Evolutionary Relationships were accessed to perform an integrated bioinformatic analysis. RIF and fertility controls patients (n = 9, respectively) were recruited from January to June 2022 at Affiliated Hospital of Changchun University of Traditional Chinese Medicine. Levels of target genes in the downstream of predicted signal pathway were measured by qPCR. Results Twenty-one different expression genes (DEGs), C11ORF65, DAPK2, KLK3, PFKFB2, SLC17A4, SLC7A1, CDC7, CDH13, DHRS3, DZIP1, GUCY1B3, KIAA0040, KIR2DL1, MAP3K5, NFKBIZ, SESTD1, TBXAS1, TIAM1, TIMP3, TNFAIP2 and TTC39C were found co-differential expression in GSE92324 and GSE103465 series, 93.19% displayed similar co-expression characteristics. In addition, the transcription factors (TFs) associated with key DEGs were recogonized, including RSF, FOR1, C/EBPALPHA, HNF-1ALPHA, AR, DP1, E2F1 and LEF1.The TFs associated diseases were focused on chromosome breakage, female infertility, embryo loss, and thrombosis, which acted a crucial role in RIF. Biological process analysis discovered that enrichment of these TFs correlated with IL-18 signal pathway, steroid hormone mediated signal pathway, DNA transcription, reproductive process and embryonic organ development. IL-18 signal pathway associated pro-immune molecules were upregulated in endometrium of RIF women than control. Conclusion The current research identified target genes and downstream signaling pathways at the maternal-fetal interface by bioinformatics-based analyses. It may provide a clue for prognosis judgement and target therapies in RIF women.
Sea level change and human occupation over 6000 years at Areoso Island (Ria de Arousa, NW Iberia)
Authors: Cajade-Pascual Daniel , Costa-Casais Manuela , Blanco-Chao Ramón , Rodríguez Teresa Taboada 
Published: January 2023

Abstract

The coast is very sensitive to variations in environmental conditions. The interaction of marine and continental processes causes a high dynamism, generating depositional formations with a high value for paleoenvironmental reconstruction. This work focused on the case of two edapho-sedimentary sequences located under the current beach, near of archaeological tumuli context at Areoso Island (Ría de Arousa, NW Iberia). With a geoarchaeological approach, sea level rise, environmental conditions and human occupation over 6000 years are interpreted. The results of granulometric and mineralogical data, elemental composition and stratigraphic features, help to identify three formation environments: granite weathering, continental (soil formation and erosion) and marine-aeolian. Geomorphological evolution from the last 6.0 kyr BP has been controlled by climate and sea level change and anthropogenic causes. The continental facies evidence low sea level up to 4.8 kyr BP. After 3.2 kyr BP, the formation of a beach-dune system was verified at an elevation similar to the present one. These results are coherent with others obtained in lagoons in the Galician coast. These edapho-sedimentary sequences located in an open coastal system will help to improve the Holocene sea level rise curve in the NW Iberia and to understand the context in which the most important archaeological structures on the island, the tumuli, began to be eroded.
Methamphetamine-induced encephalopathy in the absence of hyperammonemia
Authors: Rabbany Jessica M , Fitzgerald Kaylee , Bowman Jade , Dong Fanglong , Neeki Michael M. 
Published: January 2023

Abstract

Background: Methamphetamine is an addictive drug with various effects on the neurotransmitters in the central nervous system. Methamphetamine-induced encephalopathy in the absence of hyperammonemia presents a unique challenge in a clinical setting. Previously published cases of methamphetamine-induced encephalopathy suggested that methamphetamine-induced hepatotoxicity and subsequent hyperammonemia may be the cause of encephalopathy. However, the literature is limited on methamphetamine-induced encephalopathy without hyperammonemia. Case: This case presents a disoriented patient following methamphetamine use disorder with acute toxicity, unable to ambulate independently, and was poorly responsive to verbal stimuli. Discussion: This patient developed encephalopathy with normal liver function tests and ammonia levels suggesting a different pathophysiological pathway for methamphetamine-induced encephalopathy. One potential pathway is through the direct action of methamphetamine on the central nervous system through acute disruption of neurotransmitter signaling and disruption of the blood-brain barrier. Conclusion: Further research should be conducted into the prevalence and pathophysiology of methamphetamine-induced encephalopathy in the absence of hyperammonemia.
Large-scare subsurface flow constructed wetland for marine aquaculture water purification: functional microbial detection
Authors: Chen Chen , Li Pengquan , Zhao Weihua , Chen Jingfei , Yan Maocang , Guo Chong , Fu Min , Xu Zhenxing , Chen Xuechu 
Published: January 2023

Abstract
In order to improve the quality of natural seawater to meet the needs of aquaculture production, a large-scale subsurface flow constructed wetland was constructed and operated stably for 2 years to study its purification effect on natural seawater. Studies had shown that the system could maintain high purification effect on natural seawater during aquaculture, and could effectively remove harmful algae such as Cyanobacteria, especially in warm months. The pyrosequencing of 16S ribosomal DNA revealed that Proteobacteria, Nitrospirae, chloroflexi were the main and key bacterial phyla in the system. Members of these key gates were regarded as the important roles in resisting water purification. In summary, the subsurface flow wetland system was suitable for large-scale seawater aquaculture plants, which could effectively improve seawater quality conditions and reduced the density of harmful algae cells.
CircACLY regulates proliferation, differentiation and apoptosis of Jingyuan chicken myoblasts by sponging gga-miR-6660-3P
Authors: Zhang Juan , Wang Weizhen , Cai Zhengyun , Yu Baojun , Zhao Wei , Fu Xi , Wang Tailu , Gu Yaling , Xin Guosheng 
Published: January 2023

Abstract
Circular RNAs (circRNAs) are key gene regulators that are involved in many fundamental biological processes. Skeletal muscle cannot develop normally without the involvement of circRNA. We found that a circRNA from the ATP Citrate Lyase (ACLY) gene regulates skeletal muscle development through sponging miRNA. The results of ribonuclease R (RNase R) digestion and subcellular localization assays verified that circACLY is a circRNA that is mainly localized to the cytoplasm. Dual-luciferase and co-transfection assay findings showed that gga-miR-6660-3p binds directly to the predicted binding sites of circACLY and Ectonucleotide Triphosphate Diphosphohydrolase 7 (ENTPD7). Furthermore, circACLY increased the expression of ENTPD7 by adsorbing gga-miR-6660-3p. Cell proliferation assays showed that circACLY promoted, whereas gga-miR-6660-3p inhibited myoblast proliferation. Assays of induced differentiation, differentiation marker gene mRNA expression and protein levels showed that gga-miR-6660-3p inhibited, whereas circACLY facilitated myoblast differentiation. Furthermore, circACLY inhibited, whereas gga-miR-6660-3P promoted myoblast apoptosis, and they regulate inosine monophosphate synthesis (IMDNS) de novo by respectively promoting and inhibiting it. Our findings confirmed that circACLY from the ACLY gene regulates IMDNS by sponging- gga-miR-6660-3P to promote myoblast proliferation and differentiation and inhibit myoblast apoptosis.
Antibacterial activity of Clinoptilolite/TiO 2 /ZnO photocatalyst against Escherichia coli and Staphylococcus aureus in water resources
Authors: Abdi Maryam , Anbia Mansoor , safavi Maliheh , Sepehrian Mohammad , Nezafatian Elham 
Published: January 2023

Abstract
Water treatment is crucial due to rising water demand across the board, including drinking, agriculture, industry, etc. Pathogens are a type of contamination that must be studied to improve water purification methods. This study investigates the elimination of Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus) using Clinoptilolite/TiO2/ZnO nanocomposite with varied TiO2/ZnO ratios of 1:1, 1:2, and 2:1 under UVA and UVC light irradiation. To synergize the effect of coupling TiO2 with ZnO, we synthesized nanocomposite Clinoptilolite/TiO2/ZnO to make a powerful system for water and wastewater disinfection. The characterization tests, including X-ray diffraction, field emission scanning electron microscopy, X-ray energy diffraction, Fourier-transform infrared spectroscopy, diffuse reflection spectroscopy, and nitrogen adsorption-desorption, were performed to confirm the structure. The optimum response for bacterial removal was found by using the determination-optimal (D-optimal) design of response surface methodology (RSM). 100% removal was obtained for E. coli and S. aureus under UVA irradiation and contact time of 100 min for both of them.
The effect of twice and four activations per day of periodontal distractor on pain intensity and pulp vitality of maxillary canine
Authors: driny Alaa M. El , Salama Ahmed A. , El-Shal Osama S. , Mohamed Maha M. 
Published: January 2023

Abstract
Objectives
No study has compared pain intensity and pulp vitality of both activation protocols of periodontal distractor (twice and four activations per day) for rapid canine retraction. Since the periodontal distractor may cause some degree of suffering for the patients and may affect pulp vitality, it is important for orthodontists to handle this situation in the best possible way by choosing the most effective and the least painful method of treatment. Therefore the aim of the present study was to evaluate the effect of twice and four activations/day of periodontal distractor on pain intensity and pulp vitality of maxillary canine.
Methods
The study was carried out on 32 canine of 16 female patients (16–22 years of age) requiring extraction of bilateral maxillary first premolars and maximum anchorage. They were divided into two groups. (Group I): Canine retraction was done by periodontal distractor with twice activations per day. (Group II): Canine retraction was done by periodontal distractor with four activations per day. Periodontal distractor was cemented after bilateral maxillary premolar extraction and inter septal bone corticotomy was done. Pain intensity was evaluated at different times by Visual Analogue scale (1st 48 hour after intervention, during each activation of the distractor, and on the Interval between activation).Pulp vitality was measured by electric pulp tester.
Results
Comparison of pain intensity between different intervals was performed by using Friedman`s test (non-parametric data) which revealed significant difference in both groups, as 1st 48 hours was significantly the highest (8.8 ± 0.77), (9.2 ± 0.77) followed by During each activation (5.4 ± 0.94), (6.9 ± 1.25), while Interval between activation (1 ± 1.03), (2.7 ± 1.38) regarding group I and II respectively. Comparison between both groups was performed by using Mann Whitney`s test (non-parametric), which revealed that there was significant difference during each activation and intervals between activations as group II was significantly higher than group I (P = 0.0001).None of the teeth reacted negatively to the electrical vitality test that was performed one month after the completion of the distraction procedure. There was no clinical sign of discoloration or pulpal pain in any tooth.
Conclusion
In this study, Pain intensity was y significantly higher in group II (four activations/ day) than group I (twice activations/ day). The pulp of the retracted canine teeth examined in this study for both groups was vital on both groups.