bluuebunny/crossref_metadata_2025_split huggingface dataset:
RP-UFLC based Bioanalytical Method Development, Optimization, and
Validation for the Estimation of Isradipine in Rabbit Serum
Authors: Swain Suryakanta , Ghose Debashish , Patra Chinam Niranjan , Jena Bikash Ranjan 
Published: June 2023

Introduction:
The objective of this study is to provide a rapid, sensitive, consistent, and costeffective
method for quantifying isradipine using ultra-fast liquid chromatography.

Methods:
Quality by Design principles will form the basis of this approach, grounded on response surface
analysis. Shimadzu liquid chromatographic system equipped with a photodiode array detector and LC
solution software was used to conduct the RP-UFLC method development and validation. An ODS C18
(250 x 4.6 mm; 5 μm) UFLC column was used to complete the analysis. The RSM methodology utilized
a central composite design to perform the optimization studies.

Results:
The mobile phase ratio and flow rate were considered crucial method parameters, as well as the
peak area, retention time, and USP plate were considered critical analytical attributes. The optimal conditions
for chromatographic separation were followed using 80% acetonitrile and water (20% v/v) as mobile
phase, a 1 mL/min flow rate, an injection volume of 20 μL, 40°C of column oven temperature, and maximum
absorption at λmax 254 nm using graphical optimization technique. When examining concentrations
between 5 and 150 ng/mL, linearity was observed with an R2 of 0.999. The method created was validated
by employing stability testing per the recommendations provided by ICH Q2 (R1). The analysis of blood
serum was modified so that it could be used to examine the pharmacokinetic parameters.

Conclusion:
As a result, high accuracy, precision, sensitivity, linearity, and robustness were established
for predicting the amount of isradipine present in its freeze-dried nano-formulations.
Recent Advances in the Phylogenetic Analysis to Study Rumen
Microbiome
Authors: Wassan Jyotsna Talreja , Wang Haiying , Zheng Huiru Jane 
Published: June 2023

Background:
Recent rumen microbiome studies are progressive due to the advent of nextgeneration
sequencing technologies, computational models, and gene referencing databases. Rumen
metagenomics enables the linking of the genetic structure and composition of the rumen microbial
community to the functional role it plays in the ecosystem. Systematic investigations of the rumen microbiome,
including its composition in cattle, have revealed the importance of microbiota in rumen
functions. Various research studies have identified different types of microbiome species that reside
within the rumen and their relationships, leading to a greater understanding of their functional contribution.

Objective:
The objective of this scoping review was to highlight the role of the phylogenetic and functional
composition of the microbiome in cattle functions. It is driven by a natural assumption that closely
related microbial genes/operational taxonomical units (OTUs)/amplicon sequence variants (ASVs) by
phylogeny are highly correlated and tend to have similar functional traits.

Methods:
PRISMA approach has been used to conduct the current scoping review providing state-ofthe-
art studies for a comprehensive understanding of microbial genes’ phylogeny in the rumen microbiome
and their functional capacity.

Results:
44 studies have been included in the review, which has facilitated phylogenetic advancement in
studying important cattle functions and identifying key microbiota. Microbial genes and their interrelations
have the potential to accurately predict the phenotypes linked to ruminants, such as feed efficiency,
milk production, and high/low methane emissions. In this review, a variety of cattle have been
considered, ranging from cows, buffaloes, lambs, Angus Bulls, etc. Also, results from the reviewed literature
indicate that metabolic pathways in microbiome genomic groupings result in better carbon
channeling, thereby affecting methane production by ruminants.

Conclusion:
The mechanistic understanding of the phylogeny of the rumen microbiome could lead to a
better understanding of ruminant functions. The composition of the rumen microbiome is crucial for the
understanding of dynamics within the rumen environment. The integration of biological domain
knowledge with functional gene activity, metabolic pathways, and rumen metabolites could lead to a
better understanding of the rumen system.
Live Births in Women over 40 Years of Age Correlate with Obesity Rates
Authors: Lin Jie , Xie Qian , Xu Chaoran , Wan Qin 
Published: June 2023

Aims:
This cross-sectional study aimed to analyze the relationship between live birth and the prevalence of obesity in Chinese women over 40 years of age.

Methods:
From April to November 2011, the Endocrinology Branch of the Chinese Medical Associa-tion conducted the REACTION project, a national, multicenter, cross-sectional study of Chinese adults aged 40 years and older. Demographic and medical data were collected through validated question-naires and equipment. Anthropometric indicators, blood pressure, and biochemical data were measured by professional medical personnel. Data were analyzed using descriptive statistics and logistic analy-sis. Multivariate regression models were used to analyze obesity-related risk factors.

Results:
The prevalence of obesity among women increased gradually from 3.8% to 6.0% with an increasing number of live births. Women with two live births had the highest prevalence of over-weight at 34.3%. Overall, the obesity and overweight rates were slightly higher in premenopausal women than in postmenopausal women. Univariate regression analysis showed that the risk of obe-sity in women increased with an increasing number of live births. In addition, multivariate regres-sion analysis showed that the risk of obesity increased with an increasing number of live births in women with systolic blood pressure (SBP) < 121 mmHg or current smoking (P < 0.05).

Conclusion:
The risk of obesity increases with the number of live births in Chinese women over 40 years of age with SBP < 121 mmHg or current smoking. Our findings may facilitate the develop-ment of interventions to prevent obesity in this population.
An Overview of the Toxic Effects and Mechanisms of Zearalenone,
Aflatoxins, and Deoxynivalenol on Intestinal Integrity Damage in Humans
and Animals: A Comprehensive Review
Authors: Kumi Okyere Samuel , Hu Yanchun 
Published: June 2023

Abstract:
Mycotoxins are known to cause contamination in food crops and animal feeds. Their existence in food sources has caused several health disorders. The intestine is the first line of defense against ingested mycotoxins. Numerous studies have reported that mycotoxins impair effective digestion and absorption of nutrients in the intestinal structure, as well as affect the health and integrity of several animal species. However, the direct effect of many specific mycotoxins on the intestinal integrity factors such as the tight junction protein and gut microbiota has not been fully described. The impairment of the barrier function results in increased translocation of luminal antigens and pathogens and excessive activation of the immune system. Therefore, this review aims to provide a summary of the current evidence regarding the direct effects and mechanisms of Zearalenone, Aflatoxins, and Deoxynivalenol on intestinal integrity, focusing on epithelial cells, tight junction proteins and gut microbiota as main contributors to the development of therapeutic strategies to reduce or eliminate the harmful effects caused by mycotoxins in the intestine and targeted organs (such as liver and kidney) in the humans and animals.
Embedding components of entrepreneurship and creativity skills into construction technology education curriculum in Nigeria
Authors: Isa Umar Muhammad , Lawal Umar , Salihu Yahaya Ibn , Shuaibu Halliru , Cledumas Abdullahi Musa 
Published: June 2023

Construction Technology Education (CTE) students in tertiary institutions of Nigeria have been criticised of their inability to demonstrate entrepreneurship and creativity skills after graduation. As such wood and building construction industries in Nigeria could not employ CTE graduates. Therefore, this study aims to determine entrepreneurship and creativity skills components appropriate for embedding into the curriculum of CTE program in Nigerian tertiary institutions. A qualitative research method involving intrinsic case study design was employed for the study. The population for the study comprised 16 CTE experts purposively selected from the tertiary institutions in the north western states of Nigeria. The study utilised a semi structured interview protocol as the instrument for qualitative data collection. 3 CTE experts from Nigerian tertiary institutions validated the protocol. Data from the interview responses were manually transcribed coded and interpreted. Findings from the responses of the participants revealed marketing skills, ICT skills, management skills as part of the entrepreneurship skills components appropriate to embedded into the curriculum of CTE program. The finding further revealed that critical thinking skills, innovative skills, and self-evaluation skills formed part of the creativity skills components appropriate for embedding into the curriculum of CTE program. The study concludes by recommending to the agencies responsible with running tertiary institutions in Nigeria to do the needful in restructuring the CTE curriculum at the undergraduate level to incorporate entrepreneurship and creativity skills components for employment sustainability of CTE students after graduation. Embedding Components of Entrepreneurship and Creativity Skills into Construction Technology Education Curriculum in Nigeria
Global, Regional, and National Prevalence of Gout From 1990 to 2019: Age-Period-Cohort Analysis With Future Burden Prediction
Authors: He Qiyu , Mok Tsz-Ngai , Sin Tat-Hang , Yin Jiaying , Li Sicun , Yin Yiyue , Ming Wai-Kit , Feng Bin 
Published: June 2023

Background
Gout is a common and debilitating condition that is associated with significant morbidity and mortality. Despite advances in medical treatment, the global burden of gout continues to increase, particularly in high–sociodemographic index (SDI) regions.

Objective
To address the aforementioned issue, we used age-period-cohort (APC) modeling to analyze global trends in gout incidence and prevalence from 1990 to 2019.

Methods
Data were extracted from the Global Burden of Disease Study 2019 to assess all-age prevalence and age-standardized prevalence rates, as well as years lived with disability rates, for 204 countries and territories. APC effects were also examined in relation to gout prevalence. Future burden prediction was carried out using the Nordpred APC prediction of future incidence cases and the Bayesian APC model.

Results
The global gout incidence has increased by 63.44% over the past 2 decades, with a corresponding increase of 51.12% in global years lived with disability. The sex ratio remained consistent at 3:1 (male to female), but the global gout incidence increased in both sexes over time. Notably, the prevalence and incidence of gout were the highest in high-SDI regions (95% uncertainty interval 14.19-20.62), with a growth rate of 94.3%. Gout prevalence increases steadily with age, and the prevalence increases rapidly in high-SDI quantiles for the period effect. Finally, the cohort effect showed that gout prevalence increases steadily, with the risk of morbidity increasing in younger birth cohorts. The prediction model suggests that the gout incidence rate will continue to increase globally.

Conclusions
Our study provides important insights into the global burden of gout and highlights the need for effective management and prophylaxis of this condition. The APC model used in our analysis provides a novel approach to understanding the complex trends in gout prevalence and incidence, and our findings can inform the development of targeted interventions to address this growing health issue.
Implementation of Home-Based Telerehabilitation of Patients With Stroke in the United States: Protocol for a Realist Review
Authors: Hasan Mohamed Mosaad , Rafferty Miriam R , Tawfik Sara , Tawfik Ahmed , Beestrum Molly , Smith Justin D , Hirschhorn Lisa R , Roth Elliot J , Woods Donna M 
Published: June 2023

Background
Stroke is a common cause of mortality and morbidity. Insufficient and untimely rehabilitation has been associated with inadequate recovery. Telerehabilitation provides an opportunity for timely and accessible services for individuals with stroke, especially in remote areas. Telerehabilitation is defined as a health care team's use of a communication mode (eg, videoconferencing) to remotely provide rehabilitation services. Telerehabilitation is as effective as facility-based rehabilitation; however, it is infrequently used due to implementation barriers.

Objective
The aim of the study is to explore the interaction between the implementation strategies, context, and outcomes of telerehabilitation of patients with stroke.

Methods
This review will follow four steps: (1) defining the review scope, (2) literature search and quality appraisal, (3) data extraction and evidence synthesis, and (4) narrative development. PubMed via MEDLINE, the PEDro database, and CINAHL will be queried till June 2023 and supplemented with citation tracking and a gray literature search. The relevance and rigor of papers will be appraised using the TAPUPAS (Transparency, Accuracy, Purposivity, Utility, Propriety, Accessibility, and Specificity) and Weight of Evidence frameworks. The reviewers will extract and synthesize data iteratively and develop explanatory links between contexts, mechanisms, and outcomes. The results will be reported according to the Realist Synthesis publication standards set by Wong and colleagues in 2013.

Results
The literature search and screening will be completed in July 2023. Data extraction and analysis will be completed in August 2023, and findings will be synthesized and reported in October 2023.

Conclusions
This will be the first realist synthesis, uncovering the causal mechanisms to explain how, why, and to what extent implementation strategies impact telerehabilitation adoption and implementation.

International Registered Report Identifier (IRRID)
PRR1-10.2196/47009
Trainee Love and Breakup Letters to NephSIM
Authors: Fattah Layla , Thomas David C. , Sparks Matthew A. , Farouk Samira S. 
Published: June 2023

BackgroundIt is not known how learners feel about free open access medical education (FOAMed) as they progress through their training from medical school to fellowship. Love and breakup letter methodology (LBM) is a technique that has been used extensively in user experience technology-based research but has not previously been used in evaluating medical education tools. LBM asks participants to creatively write a “love” or “breakup” letter to a product under study to capture their thoughts and emotions when engaging with it. We conducted qualitative analysis of data from focus groups to explore how attitudes toward a learning platform change at various training stages and to broaden our understanding of how we meet learners' needs through a nephrology FOAMed tool, NephSIM.MethodsThree virtual, recorded focus groups were conducted with second-year medical students, internal medicine residents, and nephrology fellows (N=18). At the start of the focus group, participants composed and read their love and breakup letters. Semistructured discussions were then led by facilitator-driven questions and peer comments. After transcription, inductive data analysis was conducted using Braun and Clarke's six-step thematic analysis.ResultsFour main themes were seen across all groups: attitudes toward teaching tool, perception of nephrology, learning needs and approach, and application to practice. Preclinical students positively viewed the opportunity to simulate the clinical setting and unanimously wrote love letters. Reactions from residents and fellows were mixed. Residents were interested in brevity and speed of learning, preferring algorithms and succinct approaches to meet their practice-based learning needs. Fellows' learning needs were driven by a desire to prepare for the nephrology board examination and review cases uncommonly seen in practice.ConclusionsLBM provided a valuable methodology through which to identify trainee reactions to a FOAMed tool and highlighted the challenges of meeting learning needs of a continuum of trainees with a single learning platform.
Embedding Digital Fluency in Our Courses: Moving from Theory to Practice
Authors: Wells Michael 
Published: June 2023

An increasing amount of literature from academic, governmental and non-governmental organizations (Kluzer, Reyna, Hanham, & Meier, 2018; Kluzer & Pujol Priego, 2018, etc.) points to the need for post-secondary institutions to do more to prepare students to be fluent in the consumption, understanding and use of digital media and tools. Digital fluency is increasingly seen as an essential skillset for graduates’ employability and for their citizenship, but while there is strong consensus about the need for students to be more digitally fluent, there is less consensus about what digitally fluency is, or about how to teach it.
This paper will offer a working definition of digital fluency and describe an approach to fostering student digital fluency development that can be applied to a wide range of courses. Drawing on frameworks by various governmental and non-governmental organizations, as well as current scholarship in the field of teaching digital literacy (Ng, 2012.; Caufiled, 2017, Ungerer, 2016, Hinrichsen & Coombs, 2013, etc.), the paper will provide an explanation of the rationale behind the framework tool that the author has developed and the paper will conclude with an explanation of how to use the tool to embed specific and authentic digital fluency skill development at the course level.
Influence of Texture Effects on the Laser-Irradiated Tool Performance
Authors: Brover G. I. , Shcherbakova E. E. 
Published: June 2023

Introduction. Laser surface treatment of mechanical engineering products makes it possible to increase their durability. However, the laser hardening process is not good at the consistency of results, since choosing the irradiation modes and schemes of specific products, texture effects in the zones of laser exposure are not taken into account. This leads to premature wearing and even destructing the working surfaces of irradiated products. Therefore, the work objective is to study the mechanism of influence of the structure of the laser-hardened layer on the operational properties of the tool.Materials and Methods. The materials for this study were tool steels: R6M5 and R18 (according to the EN 10027 standard tool steels: 1.3355, 1.3343). Pulsed laser irradiation was carried out at the technological device Kvant-16 with a radiation power density of 70–250 MW/m2. Scanning probe and optical microscopy, X-ray diffraction and durometric methods for analyzing the steels structure were used. The values of steel strength in bending and impact strength were determined before and after laser treatment.Results. It has been experimentally proven that it is necessary to strengthen the sections of the products working surfaces that are subject to maximum wear and are under the action of compressive stresses during operation. It is shown that textural effects in the laser treatment zones lead to a decrease in the friction coefficients and contribute to an increase in the wear and adhesion resistance of the steels surface layers.Discussion and Conclusion. The results of the research carried out make it possible to rationally select the surface laser processing modes and schemes of products for various functional purposes and ensure their operability with a guarantee. The possibilities increasing the structural strength and properties of the tool due to laser alloying the surface layers of powder-coated steels and stabilizing tempering after laser irradiation are determined.
Justification of the Need to Use the Radiation and Chemical Protection Service as Part of Special Fire and Rescue Units in the Subjects of the Russian Federation
Authors: Mashtakov V. A. , Bobrinev E. V. , Udavtsova E. Yu. , Kondashov A. A. , Treshchin E. S. 
Published: June 2023

Introduction. Recently, much attention has been paid to the issues of long-term development of specialized fire and rescue units of the Federal Fire Service of the State Fire Service. In this regard, there is a need to develop criteria to justify the use of a particular service as part of specialized fire and rescue units. Therefore, the objective of this study is to develop a mathematical model to justify the need to use radiation and chemical protection services as part of specialized fire and rescue units in the subjects of the Russian Federation.Materials and Methods. Justification of the need to use radiation and chemical protection services as part of specialized fire and rescue units has been carried out using the theory of fuzzy sets. The mathematical model takes into account the climatic and geographical features of the subjects, indicators of social, technical and economic development, and the risks of emergencies and fires. It also takes into account the availability of forces and means of a Unified State system for the prevention and liquidation of emergency situations in each subject of the Russian Federation. In total, 15 indicators were selected that characterize the need to use radiation and chemical protection services as part of specialized fire and rescue units. A desirability function is defined for each indicator, which shows which values of the indicator are the most acceptable from the point of view of the need to use radiation and chemical protection services as part of specialized fire and rescue units.Results. Using the developed model, the subjects of the Russian Federation are identified in which the need for radiation and chemical protection service as part of specialized fire and rescue units is the highest. It is proposed to create a radiation and chemical protection service of the 1st category in the Moscow, Sverdlovsk and Rostov regions, in the Krasnoyarsk and Primorsky Territories and in St. Petersburg. In 21 subjects it is proposed to use the radiation and chemical protection service of the 2nd category. In other subjects, it is proposed to assign the 3rd category to the radiation and chemical protection service.Discussion and Conclusion. The mathematical model developed using the theory of fuzzy sets will allow a more differentiated approach to the creation of a radiation and chemical protection service as part of specialized fire and rescue units and increase the efficiency of the functioning of this service and specialized fire and rescue units as a whole. The presented model can be applied to justify the need to use other services and groups as part of specialized fire and rescue units.
Pemanfaatan Limbah Rumah Tangga sebagai Sumber Nutrisi dalam Kegiatan Pertanian Urban
Authors: Kamaluddin Nadia Nuraniya , Halimah Ummu ‘Azizah , Setyawan Nanda Aditya , Suryatmana Pujawati , Setiawati Mieke Rochimi 
Published: June 2023

Dalam mengatasi keterbatasan lahan hijau di perkotaan, masyarakat mulai melakukan penghijuan dipadatnya perkotaan atau biasa disebut dengan pertanian urban. Kegiatan pertanian urban dapat membantu meningkatkan udara segar dari sekian banyaknya polusi yang mengotori udara perkotaaan, mewujudkan pola hidup yang sehat, dan berkontribusi dalam menjaga ketahanan pangan. Hidroponik merupakan salah satu budidaya tanaman dengan memanfaatkan air sebagai media tanam.. Selain bahan anorganik yang dapat dimanfaatkan dalam kegiatan pertanian urban, sampah organik pun dapat diolah menjadi nutrisi bagi tanaman untuk menjadi mikroorganisme lokal. Mikroorganisme ini sangat bermanfaat bagi nutrisi tanaman. Pemanfaatan sampah organik dan anorganik ini menjadi solusi terbaik bagi masyarakat untuk mengolah limbah rumah tangga sekaligus bernilai tambah sebagai sumber nutrisi dalam pertanian urban.