bluuebunny/crossref_metadata_2025_split huggingface dataset:
A tool for nuclear imaging of the SARS-CoV-2 entry receptor: molecular model and preclinical development of ACE2-selective radiopeptides
Authors: Beyer Darja , Vaccarin Christian , Deupi Xavier , Mapanao Ana Katrina , Cohrs Susan , Sozzi-Guo Fan , Grundler Pascal V. , van der Meulen Nicholas P. , Wang Jinling , Tanriver Matthias , Bode Jeffrey W. , Schibli Roger , Müller Cristina 
Published: April 2023

Abstract
Purpose
The angiotensin converting enzyme-2 (ACE2)—entry receptor of SARS-CoV-2—and its homologue, the angiotensin-converting enzyme (ACE), play a pivotal role in maintaining cardiovascular homeostasis. Potential changes in ACE2 expression levels and dynamics after SARS-CoV-2 infection have been barely investigated. The aim of this study was to develop an ACE2-targeting imaging agent as a noninvasive imaging tool to determine ACE2 regulation.

Methods
DOTA-DX600, NODAGA-DX600 and HBED-CC-DX600 were obtained through custom synthesis and labeled with gallium-67 (T1/2 = 3.26 d) as a surrogate radioisotope for gallium-68 (T1/2 = 68 min). ACE2- and ACE-transfected HEK cells were used for the in vitro evaluation of these radiopeptides. The in vivo tissue distribution profiles of the radiopeptides were assessed in HEK-ACE2 and HEK-ACE xenografted mice and imaging studies were performed using SPECT/CT.

Results
The highest molar activity was obtained for [67Ga]Ga-HBED-CC-DX600 (60 MBq/nmol), whereas the labeling efficiency of the other peptides was considerably lower (20 MBq/nmol). The radiopeptides were stable over 24 h in saline (> 99% intact peptide). All radiopeptides showed uptake in HEK-ACE2 cells (36–43%) with moderate ACE2-binding affinity (KD value: 83–113 nM), but no uptake in HEK-ACE cells (< 0.1%) was observed. Accumulation of the radiopeptides was observed in HEK-ACE2 xenografts (11–16% IA/g) at 3 h after injection, but only background signals were seen in HEK-ACE xenografts (< 0.5% IA/g). Renal retention was still high 3 h after injection of [67Ga]Ga-DOTA-DX600 and [67Ga]Ga-NODAGA-DX600 (~ 24% IA/g), but much lower for [67Ga]Ga-HBED-CC-DX600 (7.2 ± 2.2% IA/g). SPECT/CT imaging studies confirmed the most favorable target-to-nontarget ratio for [67Ga]Ga-HBED-CC-DX600.

Conclusions
This study demonstrated ACE2 selectivity for all radiopeptides. [67Ga]Ga-HBED-CC-DX600 was revealed as the most promising candidate due to its favorable tissue distribution profile. Importantly, the HBED-CC chelator enabled 67Ga-labeling at high molar activity, which would be essential to obtain images with high signal-to-background contrast to detect (patho)physiological ACE2 expression levels in patients.
Not recommended fixed-dose antibiotic combinations in low- and middle-income countries – the example of Tanzania
Authors: Vliegenthart-Jongbloed Klaske , Jacobs Jan 
Published: April 2023

Abstract
Background
Fixed-dose combinations (FDC) are medicine formulations that combine two or more ingredients in fixed ratios in a single dose form. Although advantageous in tuberculosis and malaria (efficacy, adherence, protection against resistance), only a few antibiotic FDC (FDC-AB) have been developed along full microbiological, pharmacological and clinical validation and safety studies. The World Health Organization (WHO) database of Access, Watch and Reserve (AWaRe) antibiotics contains, since 2021, a list of “Not Recommended” FDC-AB (n = 103) which are rejected for use in clinical practice.

Body
The share of non-recommended FDC-AB in global antimicrobial use (2000–2015) was < 3% but substantially higher in middle income countries. The share increases over time, but recent data particular concerning sub-Saharan Africa are rare. Along three non-recommended FDC-AB listed in the Tanzanian National Essential Medicine List (ampicillin-cloxacillin, flucloxacillin-amoxicillin and ceftriaxone-sulbactam) we discuss the concerns and reasons behind use of these products. Non-recommended FDC-AB have poor rationale (ratios of both ingredients), lack evidence of efficacy (pharmacological, microbiological and clinical), have difficulties in dosing (underdosing of the single ingredients, absence of pediatric dosing) and risks of safety (additive toxicity). They are expected to fuel antimicrobial resistance (unnecessary broad spectrum coverage) and are incompatible with antimicrobial stewardship. The specific context of low- and middle-income countries contributes to their increased use: at the side of prescriber and supplier are the lack of diagnostics, poor training in antibiotic prescribing, patients’ preferences, role-model of senior prescribers and pharmaceutical promotion. International market mechanisms include economic motivation for development, branding and promotion, poor access to the single antibiotic forms and weak national regulatory capacity.

Conclusion and implications
There is an urgent need for monitoring consumption of non-recommended FDC-AB in low- and middle-income countries, particular in Sub-Saharan Africa. A multinational and multisectoral antimicrobial stewardship strategy is needed in order to abolish the use of non-recommended FDC-AB.
Sleep hygiene and sleep quality in Iranian adolescents during the COVID-19 pandemic
Authors: Chehri Azita , Shetabi Maryam , Khazaie Habibolah , Zakiei Ali 
Published: April 2023

Abstract
Objectives
Sleep is one of the issues that attracted the attention of researchers during the COVID-19 pandemic. Researchers focused their studies on the prevalence of sleep disorders, sleep quality, and sleep duration. Sleep hygiene is a set of guidelines that play an important role in the sleep quality, the present study sought to evaluate the amount of sleep hygiene and sleep quality in Iranian adolescents and their relationship during the COVID-19 pandemic.

Methods
The present study was conducted using a cross-sectional design. The research population consisted of all adolescents living in Kermanshah (western Iran) in 2021. The participants were a sample of 610 adolescents. They completed the Pittsburgh Sleep Quality Inventory and Adolescent Sleep Hygiene Scale.

Results
The mean sleep quality score for the participants was 7.14 ± 2.47, indicating the high frequency of sleep problems affecting the participants. There were significant correlations between all components of sleep hygiene and sleep quality. There was also a significant correlation (r = − 0.46 between sleep hygiene and sleep quality (p < 0.001). No significant difference was observed in sleep hygiene and sleep quality between the male and female adolescents. The results showed that sleep hygiene subscales can predict sleep quality (R = 0.53, F = 39.20, p < .01).

Conclusions
The data in this study confirmed the poor observance of sleep hygiene in adolescents during the COVID-19 pandemic and reported frequent sleep problems among the participants. The results also indicated a moderate relationship between sleep hygiene and sleep quality in adolescents. Thus, sleep hygiene components can be related to sleep quality.
Corrosion mechanisms for lead-barium glass from the Warring States period
Authors: Zhang Kexin , Wang Jun , Yu Wendi , Zhao Jing , Yue Xuezheng , Luo Hongjie 
Published: April 2023

Abstract
Lead-barium glass is subject to a variety of corrosion reactions after burial. The microscopic morphology and compositional structure of ancient lead-barium glass excavated from the Yaozhuang Cemetery located in Liuhe District of Nanjing were analysed with optical microscopy, X-ray fluorescence spectrometry, scanning electron microscopy equipped with energy dispersive X-ray spectroscopy, X-ray diffraction, fluorescent inverted microscopy and attenuated total reflectance-Fourier transform infrared spectroscopy. The corrosion products included PbCO3, Pb(OH)Cl, CaHPO4(H2O)2 and BaS2. Based on the corrosion morphology and the corrosion products, it was concluded that the corrosion processes included microbial corrosion, surface chemical corrosion, and crack corrosion. This paper describes the corrosion processes of lead-barium glass relics and establishes the corresponding corrosion model. In addition, the results of this study deepen the understanding of the corrosion mechanisms for lead-barium glass and provide new insight into the corrosion mechanisms of excavated glass relics, which is also of great significance for the protection of lead-barium glass.
A 12-month randomised pilot trial of the Alzheimer’s and music therapy study: a feasibility assessment of music therapy and physical activity in patients with mild-to-moderate Alzheimer’s disease
Authors: Matziorinis A. M. , Flo B. K. , Skouras S. , Dahle K. , Henriksen A. , Hausmann F. , Sudmann T. T. , Gold C. , Koelsch S. 
Published: April 2023

Abstract
Background
The Alzheimer’s and Music Therapy (ALMUTH) study is the first randomised controlled trial (RCT) design with 12 months of active non-pharmacological therapy (NPT) implementing music therapy (MT) and physical activity (PA) for participants with Alzheimer’s disease (AD). The aim of the present article is to retrospectively examine the inclusion of mild-to-moderate Alzheimer’s Disease patients into the main ALMUTH study protocol and to determine if continued inclusion of AD patients is warranted.

Methods
The randomised pilot trial was conducted as a parallel three-arm RCT, reflecting the experimental design of the ALMUTH study. The trial was conducted in Bergen, Norway, and randomisation (1:1:1) was performed by an external researcher. The study was open label and the experimental design features two active NPTs: MT and PA, and a passive control (no intervention, CON) in Norwegian speaking patients with AD who still live at home and could provide informed consent. Sessions were offered once per week (up to 90 min) up to 40 sessions over 12 months. Baseline and follow-up tests included a full neuropsychological test battery and three magnetic resonance imaging (MRI) measurements (structural, functional, and diffusion weighted imaging). Feasibility outcomes were assessed and were determined as feasible if they met the target criteria.

Results
Eighteen participants with a diagnosis of mild-to-moderate AD were screened, randomised, and tested once at baseline and once after 12-months. Participants were divided into three groups: MT (n = 6), PA (n = 6), and CON (n = 6). Results of the study revealed that the ALMUTH protocol in patients with AD was not feasible. The adherence to the study protocol was poor (50% attended sessions), with attrition and retention rates at 50%. The recruitment was costly and there were difficulties acquiring participants who met the inclusion criteria. Issues with study fidelity and problems raised by staff were taken into consideration for the updated study protocol. No adverse events were reported by the patients or their caregivers.

Conclusions
The pilot trial was not deemed feasible in patients with mild-to-moderate AD. To mitigate this, the ALMUTH study has expanded the recruitment criteria to include participants with milder forms of memory impairment (pre-AD) in addition to expanding the neuropsychological test battery. The ALMUTH study is currently ongoing through 2023.

Trial registration
Norsk Forskningsråd (NFR) funded. Regional Committees for Medical and Health Research Ethics (REC-WEST: reference number 2018/206). ClinicalTrials.gov: NCT03444181 (registered retrospectively 23 February 2018, https://clinicaltrials.gov/ct2/show/NCT03444181).
Intelligent option portfolio model with perspective of shadow price and risk-free profit
Authors: Xu Fengmin , Ma Jieao 
Published: April 2023

AbstractSince Markowitz proposed modern portfolio theory, portfolio optimization has been being a classic topic in financial engineering. Although it is generally accepted that options help to improve the market, there is still an improvement for the portrayal of their unique properties in portfolio problems. In this paper, an intelligent option portfolio model is developed that allows selling options contracts to earn option fees and considers the high leverage of options in the market. Deep learning methods are used to predict the forward price of the underlying asset, making the model smarter. It can find an optimal option portfolio that maximizes the final wealth among the call and put options with multiple strike prices. We use the duality theory to analyze the marginal contribution of initial assets, risk tolerance limit, and portfolio leverage limit for the final wealth. The leverage limit of the option portfolio has a significant impact on the return. To satisfy the investors with different risk preferences, we also give the conditions for the option portfolio to gain a risk-free return and replace the Conditional Value-at-Risk. Numerical experiments demonstrate that the intelligent option portfolio model obtains a satisfactory out-of-sample return, which is significantly positively correlated with the volatility of the underlying asset and negatively correlated with the forecast error of the forward price. The risk- free option model is effective in achieving the goal of no drawdown and gaining satisfactory returns. Investors can adjust the balance point between returns and risks according to their risk preference.
Audience immersion: validating attentional and physiological measures against self-report
Authors: Hammond Hugo , Armstrong Michael , Thomas Graham A. , Gilchrist Iain D. 
Published: April 2023

AbstractWhen an audience member becomes immersed, their attention shifts towards the media and story, and they allocate cognitive resources to represent events and characters. Here, we investigate whether it is possible to measure immersion using continuous behavioural and physiological measures. Using television and film clips, we validated dual-task reaction times, heart rate, and skin conductance against self-reported narrative engagement. We find that reaction times to a secondary task were strongly positively correlated with self-reported immersion: slower reaction times were indicative of greater immersion, particularly emotional engagement. Synchrony in heart rate across participants was associated with self-reported attentional and emotional engagement with the story, although we found no such relationship with skin conductance. These results establish both dual-task reaction times and heart rate as candidate measures for the real-time, continuous, assessment of audience immersion.
Review of sub-synchronous interaction in wind integrated power systems: classification, challenges, and mitigation techniques
Authors: Verma Neevatika , Kumar Narendra , Gupta Saket , Malik Hasmat , García Márquez Fausto Pedro 
Published: April 2023

AbstractEmerging sub-synchronous interactions (SSI) in wind-integrated power systems have added intense attention after numerous incidents in the US and China due to the involvement of series compensated transmission lines and power electronics devices. SSI phenomenon occurs when two power system elements exchange energy below the synchronous frequency. SSI phenomenon related to wind power plants is one of the most significant challenges to maintaining stability, while SSI phenomenon in practical wind farms, which has been observed recently, has not yet been described on the source of conventional SSI literature. This paper first explains the traditional development of SSI and its classification as given by the IEEE, and then it proposes a classification of SSI according to the current research status, reviews several mitigation techniques and challenges, and discusses analysis techniques for SSI. The paper also describes the effect of the active damping controllers, control scheme parameters, degree of series compensation, and various techniques used in wind power plants (WPPs). In particular, a supplementary damping controller with converter controllers in Doubly Fed Induction Generator based WPPs is briefly pronounced. This paper provides a realistic viewpoint and a potential outlook for the readers to properly deal with SSI and its mitigation techniques, which can help power engineers for the planning, economical operation, and future expansion of sustainable development.
Isolation and identification of Metarhizium guizhouense Xct1 and its pathogenicity to Spodoptera frugiperda (J.E. Smith) (Lepidoptera: Noctuidae)
Authors: Wei Juan , Wang Xian , Nima Zhuoga , Chen Lin , Song Cancan , Chen Haonan , Zhang Zhe , Qin Jie , Jiang Chunxian , Li Qing 
Published: April 2023

Abstract
Background
The fall armyworm, Spodoptera frugiperda (J.E. Smith) (Lepidoptera: Noctuidae), is one of the most destructive invasive pests causing serious damage to maize crops in China. At present, the use of chemical pesticides is the main method to prevent and control S. frugiperda. However, the excessive use of chemical pesticides not only causes toxicity in humans and animals but also pollutes the environment and causes public harm. In this context, the present study aimed to isolate, identify and evaluate the pathogenicity of the native strain of Metarhizium guizhouense against eggs, larvae and pupae of S. frugiperda under laboratory conditions.

Results
The fungal isolate was identified as M. guizhouense (Xct1) based on its morphological and molecular characteristics by sequencing ITS, β-tubulin and EF1-α gene. Meanwhile, the fungal isolate was highly pathogenic to eggs of the S. frugiperda, at the highest concentration of 1 × 108 spores/ml, the cumulative egg hatching rate was only 8.33% at 10 days post exposure. After 10 days of exposure to the highest concentration of 1 × 108 spores/ml, the LC50 values for the first–fifth larval instars of S. frugiperda were 8.12 × 104, 1.11 × 105, 5.55 × 105, 3.87 × 106 and 1.43 × 107 spores/ml, respectively.

Conclusions
The pathogenicity of M. guizhouense Xct1 to S. frugiperda larvae gradually decreased with increasing the developmental stages. Metarhizium guizhouense Xct1 was more pathogenic to egg and younger larvae of S. frugiperda. This provides a basis for determining the optimal period of control.
Adaptive partitioning-based discrete unified gas kinetic scheme for flows in all flow regimes
Authors: Yang L. M. , Han L. C. , Ding H. , Li Z. H. , Shu C. , Liu Y. Y. 
Published: April 2023

AbstractTo improve the efficiency of the discrete unified gas kinetic scheme (DUGKS) in capturing cross-scale flow physics, an adaptive partitioning-based discrete unified gas kinetic scheme (ADUGKS) is developed in this work. The ADUGKS is designed from the discrete characteristic solution to the Boltzmann-BGK equation, which contains the initial distribution function and the local equilibrium state. The initial distribution function contributes to the calculation of free streaming fluxes and the local equilibrium state contributes to the calculation of equilibrium fluxes. When the contribution of the initial distribution function is negative, the local flow field can be regarded as the continuous flow and the Navier–Stokes (N-S) equations can be used to obtain the solution directly. Otherwise, the discrete distribution functions should be updated by the Boltzmann equation to capture the rarefaction effect. Given this, in the ADUGKS, the computational domain is divided into the DUGKS cell and the N-S cell based on the contribution of the initial distribution function to the calculation of free streaming fluxes. In the N-S cell, the local flow field is evolved by solving the N-S equations, while in the DUGKS cell, both the discrete velocity Boltzmann equation and the corresponding macroscopic governing equations are solved by a modified DUGKS. Since more and more cells turn into the N-S cell with the decrease of the Knudsen number, a significant acceleration can be achieved for the ADUGKS in the continuum flow regime as compared with the DUGKS.
Role of intramuscular progesterone supplementation on the day of embryo transfer in artificial frozen cycles
Authors: Bakry Ahmed , Eldesouky Abdelfatah , Abu-hamila Fouad , Mowafy Aly Hossam , Abdel-Rasheed Mazen , Fahmy Radwa M. 
Published: April 2023

Abstract
Background
Sufficient endometrial preparation with or without progesterone supplementation is crucial in artificial cycles with frozen embryo transfer (FET). We aimed to study the effect of intramuscular progesterone supplementation on the day of embryo transfer (ET) in artificial frozen cycles.

Methods
A clinical cohort study involved women undergoing FET with artificially prepared endometrium. Serum progesterone levels were assessed on the day of ET. Accordingly, we recruited 177 women with progesterone levels more than 9.2 ng/ml in group 1, and 177 other women with progesterone levels less than 9.2 ng/ml in group 2. Women in group 1 received only 400 mg vaginal progesterone twice-daily after ET, while women in group 2 received additional intramuscular progesterone supplementation. The chemical, clinical, and ongoing pregnancy rates, as well as the pregnancy loss rate, were assessed in both groups.

Results
Expectantly, both groups showed a significant difference regarding the serum progesterone level on the day of ET (13.43 ± 4.65 vs 4.62 ± 2.77, P = 574). However, with additional intramuscular progesterone supplementation in group 2, both groups showed no significant difference regarding the chemical pregnancy rate (68.93% in group 1 vs 63.84% in group 2, P = 0.311), the clinical pregnancy rate (61.02% in group 1 vs 58.76% in group 2, P = 0.664), ongoing pregnancy rate (56.50% in group 1 vs 53.11% in group 2, P = 0.522), and pregnancy loss rate (7.41% in group 1 vs 9.62% in group 2, P = 0.564).

Conclusions
Intramuscular progesterone supplementation in women with decreased serum progesterone levels could improve pregnancy outcomes in artificial frozen cycles.

Trial registration
It was first registered at ClinicalTrials.gov on 8/4/2021 with registration number NCT04837768.
Quantifying the psychological and behavioural consequences of a diagnostic label for non-cancer conditions: systematic review
Authors: Sims Rebecca , Michaleff Zoe A. , Glasziou Paul , Jones Mark , Thomas Rae 
Published: April 2023

Background
Screening for asymptomatic health conditions is perceived as mostly beneficial, with possible harms receiving little attention.

Aims
To quantify proximal and longer-term consequences for individuals receiving a diagnostic label following screening for an asymptomatic, non-cancer health condition.

Method
Five electronic databases were searched (inception to November 2022) for studies that recruited asymptomatic screened individuals who received or did not receive a diagnostic label. Eligible studies reported psychological, psychosocial and/or behavioural outcomes before and after screening results. Independent reviewers screened titles and abstracts, extracted data from included studies, and assessed risk of bias (Risk of Bias in Non-Randomised Studies of Interventions). Results were meta-analysed or descriptively reported.

Results
Sixteen studies were included. Twelve studies addressed psychological outcomes, four studies examined behavioural outcomes and none reported psychosocial outcomes. Risk of bias was judged as low (n = 8), moderate (n = 5) or serious (n = 3). Immediately after receiving results, anxiety was significantly higher for individuals receiving versus not receiving a diagnostic label (mean difference −7.28, 95% CI −12.85 to −1.71). On average, anxiety increased from the non-clinical to clinical range, but returned to the non-clinical range in the longer term. No significant immediate or longer-term differences were found for depression or general mental health. Absenteeism did not significantly differ from the year before to the year after screening.

Conclusions
The impacts of screening asymptomatic, non-cancer health conditions are not universally positive. Limited research exists regarding longer-term impacts. Well-designed, high-quality studies further investigating these impacts are required to assist development of protocols that minimise psychological distress following diagnosis.