bluuebunny/crossref_metadata_2025_split huggingface dataset:
Associations of periconceptional oral contraceptive use with pregnancy complications and adverse birth outcomes
Authors: Schreuder Anton , Mokadem Ibtissam , Smeets Nori J L , Spaanderman Marc E A , Roeleveld Nel , Lupattelli Angela , van Gelder Marleen M H J 
Published: April 2023

Abstract

Background
Periconceptional use of oral contraceptives (OCs) has been reported to increase risks of pregnancy complications and adverse birth outcomes, but risks are suggested to differ depending on the timing of discontinuation, amount of oestrogen and progestin content.

Methods
Prospective cohort study among 6470 pregnancies included in the PRegnancy and Infant DEvelopment (PRIDE) Study in 2012–19. Exposure was defined as any reported use of OCs within 12 months pre-pregnancy or after conception. Outcomes of interest were gestational diabetes, gestational hypertension, pre-eclampsia, pre-term birth, low birthweight and small for gestational age (SGA). Multivariable Poisson regression using stabilized inverse probability weighting estimated relative risks (RRs) with 95% CIs.

Results
Any periconceptional OC use was associated with increased risks of pre-eclampsia (RR 1.38, 95% CI 0.99–1.93), pre-term birth (RR 1.38, 95% CI 1.09–1.75) and low birthweight (RR 1.45, 95% CI 1.10–1.92), but not with gestational hypertension (RR 1.09, 95% CI 0.91–1.31), gestational diabetes (RR 1.02, 95% CI 0.77–1.36) and SGA (RR 0.96, 95% CI 0.75–1.21). Associations with pre-eclampsia were strongest for discontinuation 0–3 months pre-pregnancy, for OCs containing ≥30 µg oestrogen and for first- or second-generation OCs. Pre-term birth and low birthweight were more likely to occur when OCs were discontinued 0–3 months pre-pregnancy, when using OCs containing &lt;30 µg oestrogen and when using third-generation OCs. Associations with SGA were observed for OCs containing &lt;30 µg oestrogen and for third- or fourth-generation OCs.

Conclusions
Periconceptional OC use, particularly those containing oestrogen, was associated with increased risks of pre-eclampsia, pre-term birth, low birthweight and SGA.
On Multiplicative Functions with Small Partial Sums
Authors: Sachpazis Stelios 
Published: April 2023

Abstract
In analytic number theory, several results make use of information regarding the prime values of a multiplicative function in order to extract information about its averages. Examples of such results include Wirsing’s theorem and the Landau–Selberg–Delange method. In this paper, we are interested in the opposite direction. In particular, we prove that when $f$ is a suitable divisor-bounded multiplicative function with small partial sums, then $f(p)\approx -p^{i\gamma _1}-\ldots -p^{i\gamma _m}$ on average, where the $\gamma _j$s are the imaginary parts of the zeros of the Dirichet series of $f$ on the line $\textrm {Re}(s)=1$. This extends a result of Koukoulopoulos and Soundararajan and it builds upon ideas coming from previous work of Koukoulopoulos for the case where $|f|\leqslant 1$.
European Regional International Society and the Political Economy of the Global Sugar Regime
Authors: Andrieu Kieran , Lubbock Rowan 
Published: April 2023

AbstractThis paper seeks to contribute to the English School's (ES) understanding of the European Regional International Society (ERIS) through the work of Karl Polanyi. While ES theory has long been interested in regional international societies, its general approach remains limited to a methodologically internationalist frame that fails to capture the dynamism and historical change of regional formations. We therefore aim to better ground the ES account of the ERIS within a more robust political economy framework that incorporates domestic dynamics with international processes. The article first examines the making of the nineteenth-century liberal order and its eventual breakdown during the turn of the century—the “great transformation”, which ultimately informed the rationale for the European Community (EC). We then focus our analysis on the EC's Common Agricultural Policy. With specific examination of sugar production, we explore the tensions and contradictions bound up with the formation of a protected domestic and regional sugar market, the pressures it exerted on the wider international society, and the ways in which European officials skillfully exploited the post-Cold War liberalization of international society as a means of (partially) “disembedding” European sugar. Lastly, we hope that this article begins a new conversation on how the tenets of political economy (Polanyian or otherwise) might, at long last, make an impact on ES theory.
Genetic and biochemical characterization of BIM-1, a novel acquired subgroup B1 MBL found in a Pseudomonas sp. strain from the Brazilian Amazon region
Authors: Souza Cintya O , Cayô Rodrigo , Lima Karla Valéria B , Brasiliense Danielle M , Streling Ana Paula , Siqueira André V , Alberto-Lei Felipe , Leal Josiane T , Nodari Carolina S , Pérez-Chaparro Paula Juliana , Lima Luana N G C , Lima Marcelo O , Costa Brenda Natasha S , De Queiroz Thais Karolina L , Silva Paola J S N , Mamizuka Elza M , Marcondes Marcelo F , Mcculloch John Anthony , Gales Ana Cristina 
Published: April 2023

Abstract

Objectives
To characterize a novel acquired MBL, BIM-1, in a Pseudomonas #2 (subgroup P. guariconensis) strain isolated from the Aurá river located in the Brazilian Amazon hydrographic basin.

Methods
WGS using an Illumina® MiSeq System was used to characterize the genome of Pseudomonas sp. IEC33019 strain. Southern blotting/hybridization assays were performed to confirm the location of the MBL-encoding gene, blaBIM-1 (Belém Imipenemase). Antimicrobial susceptibility testing, cloning, and biochemical and phenotypic characterization were performed to determine BIM-1 kinetics.

Results
The IEC33019 strain showed high resistance rates to β-lactams, ciprofloxacin and aminoglycosides, being susceptible only to polymyxins and susceptible, increased exposure to aztreonam. WGS analysis revealed a novel acquired MBL-encoding gene, blaBIM-1, found as a gene cassette inserted into a class 1 integron (In1326) that also carried qnrVC1 and aadA11e. In1326 was located in a complex transposon, Tn7122, carried by a 52.7 kb conjugative plasmid (pIEC33019) with a toxin/antitoxin system (vapB/vapC). BIM-1 belongs to the molecular subgroup B1 and shares 70.2% and 64.9% similarity with SIM-1 and IMP-1, respectively. Kinetics analysis of BIM-1 showed hydrolytic activity against all β-lactams tested.

Conclusions
BIM-1 is a novel acquired MBL encoded by a gene carried by mobile genetic elements, which can be transferred to other Gram-negative bacilli (GNB). Because the IEC33019 strain was recovered from a river impacted by a populous metropolitan region with poor basic sanitation and served by limited potable freshwater, it would be important to establish the role of the BIM-1-producing GNB as nosocomial pathogens and/or as colonizers of the riverside population in this geographical region.
Drug–drug interaction: decreased posaconazole trough concentrations during concomitant flucloxacillin treatment
Authors: Wortman Julia M , Leegwater Emiel , Van Lammeren-Venema Daniëlle , Van Nieuwkoop Cees , Sobels Annemieke , Wilms Erik B 
Published: April 2023

Abstract

Background and objectives
Posaconazole is used as prophylaxis of invasive fungal disease in immune-compromised haematological patients with prolonged neutropenia after intensive chemotherapy. During routine therapeutic drug monitoring of posaconazole, we repeatedly observed low posaconazole serum concentrations in patients that were concomitantly treated with flucloxacillin. A possible interaction between flucloxacillin and posaconazole was explored in this case series.

Patients and methods
Posaconazole trough serum concentrations during and before/after flucloxacillin treatment were collected from 10 patients.

Results
With a median concentration of 0.5 mg/L (IQR 0.3–0.6), the posaconazole trough serum concentration decreased by 47% during flucloxacillin treatment compared with the concentration before/after flucloxacillin treatment (0.9 mg/L, IQR 0.6–1.3). As a result, the posaconazole target trough concentration of ≥0.7 mg/L was only achieved in five out of nine patients during flucloxacillin treatment.

Conclusions
Careful monitoring of posaconazole serum trough concentrations is recommended when concomitant use of flucloxacillin cannot be avoided.
3,5-bis(styryl)pyrazole inhibits mitosis and induces cell death independent of BubR1 and p53 levels by depolymerizing microtubules
Authors: Batra Pooja J , Kumari Anuradha , Liao Vivian W Y , Hibbs David E , Groundwater Paul W , Panda Dulal 
Published: April 2023

Abstract
Here, we show that 3,5-bis[(1E)-2-(2,6-dichlorophenyl)ethenyl]-1H-pyrazole 2l depolymerizes microtubules and reduces the number of growing tips of microtubules. The fluorescence recovery after photobleaching experiment in live MCF-7 cells showed that pyrazole 2l suppresses spindle microtubule dynamics. Further, the compound inhibits chromosome movements, activates the spindle assembly checkpoint and blocks mitosis in MCF-7 cells. Pyrazole 2l treatment induced cell death in a variety of pathways. Pyrazole 2l induces cell death independent of BubR1 and p53 levels of MCF-7 cells upon microtubule depolymerization. Further, pyrazole 2l increases the interaction between NF-κB and microtubules and enhances the nuclear localization of NF-κB at its half-maximal proliferation inhibitory concentration while a high concentration of the compound reduced the nuclear localization of NF-κB. Interestingly, the compound exerted significantly stronger antiproliferative effects in cancerous cells than in non-cancerous cells. The results indicated that pyrazole 2l inhibits mitosis by targeting microtubules, induces several types of cell death stimuli and suggests its potential as a lead in developing anticancer agent.
Clinical practice of UroVysion® urine test in patients with bladder carcinoma in situ treated with intravesical Bacillus Calmette–Guerin
Authors: Miyake Makito , Hori Shunta , Fujii Tomomi , Nishimura Nobutaka , Oda Yuki , Miyamoto Tatsuki , Tomizawa Mitsuru , Shimizu Takuto , Ohnishi Kenta , Morizawa Yosuke , Gotoh Daisuke , Nakai Yasushi , Torimoto Kazumasa , Tanaka Nobumichi , Fujimoto Kiyohide 
Published: April 2023

Abstract
In January 2019, the use of the UroVysion® urine test for surveillance of non-muscle invasive bladder cancer with carcinoma in situ (CIS) was approved in Japan. Clinical evidence of its use remains limited. Herein, we report the real-world clinical practice of the UroVysion test. Of 29 patients underwent at least one UroVysion test at our hospital from 2019 to 2022, only two (6.9%) tested positive without any visible tumor on the cystoscopy after the initial transurethral resection: a 77-year-old man with T1 high-grade tumor and concomitant CIS and a 76-year-old woman with CIS. The remaining 27 patients (93.1%) tested negative post-transurethral resection. This study was the first to report the Japanese real-world practice of the UroVysion test, demonstrating relatively low positive rate as compared to the previous reports from other countries. Further clinical evidence from other Japanese institutes needs to be accumulated to update the true value of this test.
Settlement Around the World: Settlement Rates in the Largest Economies
Authors: Chang Yun-chien , Klerman Daniel 
Published: April 2023

Abstract
This article presents the first rigorous, multi-country analysis of settlement rates. Data on settlement were collected for twenty-three of the twenty-five largest economies. Settlement rates vary greatly, from below 15% in France, Belgium, and Russia, to over two-thirds in Australia. Settlement rates also vary by subject matter, with settlement rates generally highest in tort, especially in the U.S. There are also dramatic differences in settlement rates by legal origin, with common law countries generally having the highest settlement rates. Because settlement rates in civil law countries are so low, selection effects may not be a significant problem for empirical work involving them. Higher settlement rates are correlated with early disclosure and shorter court delays, but more research is needed to explain the large differences in settlement rates.
In vitro simulation of the acute lymphoblastic leukemia niche: a critical view on the optimal approximation for drug testing
Authors: Pottosin Igor , Olivas-Aguirre Miguel , Dobrovinskaya Oxana 
Published: April 2023

Abstract
Acute lymphoblastic leukemia with the worst prognosis is related to minimal residual disease. Minimal residual disease not only depends on the individual peculiarities of leukemic clones but also reflects the protective role of the acute lymphoblastic leukemia microenvironment. In this review, we discuss in detail cell-to-cell interactions in the 2 leukemic niches, more explored bone marrow and less studied extramedullary adipose tissue. A special emphasis is given to multiple ways of interactions of acute lymphoblastic leukemia cells with the bone marrow or extramedullary adipose tissue microenvironment, indicating observed differences in B- and T-cell–derived acute lymphoblastic leukemia behavior. This analysis argued for the usage of coculture systems for drug testing. Starting with a review of available sources and characteristics of acute lymphoblastic leukemia cells, mesenchymal stromal cells, endothelial cells, and adipocytes, we have then made an update of the available 2-dimensional and 3-dimensional systems, which bring together cellular elements, components of the extracellular matrix, or its imitation. We discussed the most complex available 3-dimensional systems like “leukemia-on-a-chip,” which include either a prefabricated microfluidics platform or, alternatively, the microarchitecture, designed by using the 3-dimensional bioprinting technologies. From our analysis, it follows that for preclinical antileukemic drug testing, in most cases, intermediately complex in vitro cell systems are optimal, such as a “2.5-dimensional” coculture of acute lymphoblastic leukemia cells with niche cells (mesenchymal stromal cells, endothelial cells) plus matrix components or scaffold-free mesenchymal stromal cell organoids, populated by acute lymphoblastic leukemia cells. Due to emerging evidence for the correlation of obesity and poor prognosis, a coculture of adipocytes with acute lymphoblastic leukemia cells as a drug testing system is gaining shape.
G protein-coupled receptor–mediated membrane targeting of PLCγ2 is essential for neutrophil chemotaxis
Authors: Xu Xuehua , Wen Xi , Bhimani Smit , Moosa Amer , Parsons Dustin , Ha HyunGee , Jin Tian 
Published: April 2023

Abstract
The current dogma is that chemoattractants G protein-coupled receptors activate β phospholipase C while receptor tyrosine kinases activate γ phospholipase C. Here, we show that chemoattractant/G protein-coupled receptor-mediated membrane recruitment of γ2 phospholipase C constitutes G protein-coupled receptor-mediated phospholipase C signaling and is essential for neutrophil polarization and migration during chemotaxis. In response to a chemoattractant stimulation, cells lacking γ2 phospholipase C (plcg2kd) displayed altered dynamics of diacylglycerol production and calcium response, increased Ras/PI3K/Akt activation, elevated GSK3 phosphorylation and cofilin activation, impaired dynamics of actin polymerization, and, consequently, defects in cell polarization and migration during chemotaxis. The study reveals a molecular mechanism of membrane targeting of γ2 phospholipase C and the signaling pathways by which γ2 phospholipase C plays an essential role in neutrophil chemotaxis.
Inverse agonists of retinoic acid receptor/retinoid X receptor signaling as lineage-specific antitumor agents against human adenoid cystic carcinoma
Authors: Viragova Sara , Aparicio Luis , Palmerini Pierangela , Zhao Junfei , Valencia Salazar Luis E , Schurer Alexandra , Dhuri Anika , Sahoo Debashis , Moskaluk Christopher A , Rabadan Raul , Dalerba Piero 
Published: April 2023

AbstractBackgroundAdenoid cystic carcinoma (ACC) is a lethal malignancy of exocrine glands, characterized by the coexistence within tumor tissues of 2 distinct populations of cancer cells, phenotypically similar to the myoepithelial and ductal lineages of normal salivary epithelia. The developmental relationship linking these 2 cell types, and their differential vulnerability to antitumor treatments, remains unknown.MethodsUsing single-cell RNA sequencing, we identified cell-surface markers (CD49f, KIT) that enabled the differential purification of myoepithelial-like (CD49fhigh/KITneg) and ductal-like (CD49flow/KIT+) cells from patient-derived xenografts (PDXs) of human ACCs. Using prospective xenotransplantation experiments, we compared the tumor-initiating capacity of the 2 cell types and tested whether one could differentiate into the other. Finally, we searched for signaling pathways with differential activation between the 2 cell types and tested their role as lineage-specific therapeutic targets.ResultsMyoepithelial-like cells displayed higher tumorigenicity than ductal-like cells and acted as their progenitors. Myoepithelial-like and ductal-like cells displayed differential expression of genes encoding for suppressors and activators of retinoic acid signaling, respectively. Agonists of retinoic acid receptor (RAR) or retinoid X receptor (RXR) signaling (all-trans retinoic acid, bexarotene) promoted myoepithelial-to-ductal differentiation, whereas suppression of RAR/RXR signaling with a dominant-negative RAR construct abrogated it. Inverse agonists of RAR/RXR signaling (BMS493, AGN193109) displayed selective toxicity against ductal-like cells and in vivo antitumor activity against PDX models of human ACC.ConclusionsIn human ACCs, myoepithelial-like cells act as progenitors of ductal-like cells, and myoepithelial-to-ductal differentiation is promoted by RAR/RXR signaling. Suppression of RAR/RXR signaling is lethal to ductal-like cells and represents a new therapeutic approach against human ACCs.
Women’s disordered eating and sexual function: the role of interoception
Authors: Poovey Kendall , de Jong David , Rancourt Diana 
Published: April 2023

Abstract

Background
Among women, disordered eating and sexual difficulties commonly co-occur.

Aim
We investigated whether interoceptive dysfunction (ie, altered processing of body cues) may be a common cause (ie, third variable) contributing to these concomitant outcomes above and beyond body mass index (BMI) and body image concerns.

Methods
Women (n = 1201; mean age = 25.92 years; mean BMI = 25.46 kg/m2; 61.6% heterosexual, 27.5% bisexual) were recruited from sexual education courses at a midsize southeastern university and Reddit communities related to women’s health, sex, and disordered eating. Participants completed online measures of self-reported interoception (Multidimensional Assessment of Interoceptive Awareness Version 2), disordered eating attitudes and behaviors (Eating Disorder Examination Questionnaire), sexual function (Female Sexual Function Index), and demographic information, including height and weight. Regression, commonality, and dominance analyses were completed in R.

Outcomes
Sexual function was assessed via the Female Sexual Function Index.

Results
Both general interoception and body trust (a facet of interoception concerned with trust of internal sensations) predicted dietary restraint and sexual function (Ps &lt; .001). After accounting for both general interoception (β = 0.27, P &lt; .001) and body trust (β = 0.32, P &lt; .001) in hierarchal regression models predicting sexual function, dietary restraint either evidenced a reduction in its standardized beta or was no longer associated with sexual function. Body image concerns also emerged as a robust predictor of women’s sexual function. Body trust accounted for the most unique variance (4.45%) in women’s sexual function and commonly accounted for another 3.70% of variance with body image concerns.

Clinical Implications
Interoceptive exposure interventions may contribute to better outcomes for women with disordered eating, sexual difficulties, or co-occurring concerns, a notion worthy of future investigation.

Strengths and Limitations
Strengths include a large diverse sample in terms of sexual orientation (&gt;35% sexual minority) and body size (BMIs from underweight to class 3 obesity). The cross-sectional nature of this study precludes any conclusions regarding temporal precedence or causality. Future studies should use different measures of dietary restraint for nonclinical populations (eg, objective measures of caloric intake).

Conclusion
Findings suggest that both interoception and body image concerns contribute to the co-occurrence of disordered eating behaviors and sexual difficulties, and that both may be important risk/maintaining factors to consider in future work. Interoception should be integrated into models of women’s sexual function, as it may be just as important to women’s sexual well-being as body image concerns.
Development and properties evaluation of multi-strain probiotic with cholesterol-lowering potentialin vitro
Authors: Zhao Peng , Li Mingxing , Mo Xiang , Yang Junwei , Liu Lifeng , Huang Yuanxia , Qiu Zhongping 
Published: April 2023

AbstractProbiotics have a cholesterol-lowering effect. Multi-strain probiotics (MSPs) outperformed single-strain probiotics due to the interaction of strains. Hence, we expect to develop a high-efficiency MSP for lowering cholesterol. Three strains, including Lactobacillus caseiS1, Enterococcus faeciumS4, and L. harbinensisS6, were used to develop the MSPs. To evaluate their effect, gastrointestinal tolerance, bile salt hydrolase (BSH) activity, cholesterol-lowering rate, antioxidant ability, gamma-aminobutyric acid (GABA) production, and antibiotic sensitivity were determined. The triple lactic acid bacteria probiotic (TLP) was the most efficient one. After 24 h treatment with artificial gastric fluid, 47.88% of TLP survived. TLP exhibited the highest BSH activity (149.40 U·ml−1) and cholesterol-lowering rate (75.05%) in vitro, with co-precipitation reducing the majority of cholesterol. The reducing power and superoxide radical scavenging rate of the cell-free supernatant and the hydroxyl radical scavenging rate in viable cells were 1.52, 85.03%, and 89.66%, respectively, and the GABA production was 0.67 mg·ml−1, which enhanced the health benefit effects. By fuzzy mathematical analysis, TLP was the optimal probiotic and was competitive with commercial probiotics. The three strains were susceptible to 13 antibiotics. Therefore, TLP has the potential to develop into a cholesterol-lowering probiotic preparation.
The correlation between denaturation of glyceraldehyde-3-phosphate dehydrogenase and inactivation ofSaccharomyces pastorianuscells by pressurized carbon dioxide microbubbles
Authors: Takahashi Konomi , Kobayashi Fumiyuki , Narai-Kanayama Asako , Odake Sachiko 
Published: April 2023

AbstractFor the purpose of clarifying the relationship between pasteurization and inactivation of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) in Saccharomyces pastorianus cells induced by pressurized carbon dioxide microbubbles (CO2MB) treatment, a storage test of S. pastorianus cells after CO2MB treatment was conducted to ascertain their recovery, and the treatment condition in the inactivation of GAPDH in S. pastorianus cells by CO2MB was investigated. Each population of S. pastorianus for 48, 96, and 144 h at 25°C was decreased significantly by CO2MB treatment at 35°C for 3 min (MB35-3 and MB35-5) or at 40°C and 45°C for 1 and 3 min (MB40-1, MB40-3, and MB45-1). In the storage test, recovery of treated cells was not observed after storage for 144 h at 25°C. The denaturation of GAPDH in the S. pastorianus cells caused by the same treatment as the storage test was detected by using sodium dodecyl sulphate polyacrylamide gel electrophoresis. While the activities at MB35-1, MB35-3, and MB40-1 were significantly higher than those at non-treatment, and those at MB35-5, MB40-3, and MB45-1 were lower. Therefore, GAPDH denaturation, but not the activity, was associated with the inactivation of S. pastorianus cells.