bluuebunny/crossref_metadata_2025_split huggingface dataset:
Migraine Frequency and Duration Among Medical Students Before Matriculation Versus After Matriculation: A Cross-Sectional Survey Design
Authors: Reddy Amsha 
Published: March 2023

Abstract
Background: The aim of this study was to evaluate the frequency and duration of migraine among medical students following matriculation into medical school compared to frequency and duration prior to matriculation. This study also evaluated the relationship of post-matriculation frequency and duration of migraine with potential triggers including perceived stress, average hours of sleep per night, and daily water consumption.
Methods: This study employed a cross-sectional survey design and included 78 participants enrolled at the Alabama College of Osteopathic Medicine (ACOM) in Dothan, Alabama for the 2022-2023 academic year. Data was collected from participants’ self-reporting answers to each survey item and analyzed using two factor ANOVA, paired two sample t-test, and Chi-square test of independence.
Results: Participants demonstrated increased duration of migraine (p value = .03), increased water consumption (p value = .008), and increased perceived stress post-matriculation (p value < .001). Increased migraine duration post-matriculation was not found to be associated with the increase in perceived stress. Migraine frequency did not increase post-matriculation. However, participants who reported less hours of sleep were more likely to have increased frequency of migraine post-matriculation (p = .007). Female participants were more likely to have increased frequency (p = .002) and duration (p = .003) of migraine post-matriculation. Participants with family history of migraine were also more likely to have increased frequency (p = .046) and duration (p = .001) of migraine post-matriculation.
Conclusions: This study revealed greater duration of migraine among medical students following matriculation into medical school compared to duration of migraine pre-matriculation.
Contralateral Translaminar Endoscopic Approach for Highly Down-migrated Lumbar Disc Herniation using Percutaneous Biportal Endoscopic Surgery
Authors: Cheng Wei , Gao Wenshuo , Zhu Chengyue , Shao Rongxue , Wang Dong , Pan Hao , Zhang Wei 
Published: March 2023

Abstract
Objective: Unilateral biportal endoscopy （UBE）is a minimally invasive spine surgery with reduced traumatization of the posterior lumbar ligament and muscular structures. This study reports contralateral translaminar approach with UBE for highly down-migrated lumbar disc herniation (LDH).
Methods: Data of 32 patients with highly down-migrated LDH treated using UBE at Hangzhou TCM hospital from January 2020 to July 2022 were retrospectively analyzed. The operation time and perioperative complications were recorded, and the visual analog scale (VAS) of pain was recorded to evaluate the degree of lower back and extremity pain. The Oswestry disability index (ODI) was used to evaluate lumbar spine function. The modified MacNab score was used to evaluate clinical efficacy.
Results: All patients successfully underwent the operation, with a time range from 47 to 65 minutes and an average operation time of 56.09 ±5.11 minutes. Overall, 17 and 15 were males and females, respectively, with ages ranging from 34 to 72 years and an average age of 56 ±7.89 years. The postoperative follow-up period was 12–18 months, with an average of 14.9 ±2.3 months. The postoperative lower back VAS pain score and ODI were statistically significant compared with preoperatively (P < 0.05). At the final follow-up, according to the modified Macnab criteria, 90.6% of cases were classified as good or excellent.
Conclusion: UBE treatment of highly down-migrated LDH through the contralateral translaminar approach is safe and efficient. Therefore, this approach can be an efficient alternative for patients with highly downward-migrating LDH.
Stratum corneum lipidomics analysis of the transdermal permeability achieved by phytosterol-mediated glycerosomes combined with peppermint oil
Authors: Ruan Hang , Shen Lina , Hou Xiaolin , Li Jiaqi , Guo Teng , Zhu Chunyun , Feng Nianping , Zhang Yongtai 
Published: March 2023

Abstract
Although the introduction of glycerosomes have enriched strategies for efficient transdermal drug delivery, the inclusion of cholesterol as a membrane stabilizer has limited their clinical application. The current study describes the development and optimization of a new type of glycerosome (S-glycerosome) that is formed in glycerol solution with β-sitosterol as the stabilizer. Moreover, the transdermal permeation properties of lappaconitine (LA)-loaded S-glycerosomes and peppermint oil (PO)-mediated S-glycerosomes (PO-S-glycerosomes) are evaluated, and the lipid alterations in the stratum corneum are analyzed via lipidomics. Results show that the LA-loaded S-glycerosomes have a mean size of 145.3 ± 7.81 nm and encapsulation efficiency of 73.14 ± 0.35%. Moreover, the addition of PO positively impacts transdermal flux, peaking at 0.4% (w/v) PO. Tracing of the fluorescent probe P4 further revealed that PO-S-glycerosomes penetrate deeper into the skin than S-glycerosomes and conventional liposomes. Additionally, treatment with PO-S-glycerosomes alters the isoform type, number, and composition of sphingolipids, glycerophospholipids, glycerolipids, and fatty acids in the stratum corneum, with the most notable effect observed for ceramides, the main component of sphingolipids. Furthermore, the administration of PO-S-glycerosomes improves the treatment efficacy of xylene-induced inflammation in mice. Collectively, these findings demonstrate the feasibility of β-sitosterol as a stabilizer in glycerosomes. Additionally, the inclusion of PO improves the transdermal permeation of S-glycerosomes, potentially by altering the stratum corneum lipids.
miR-33a-3p regulates METTL3-mediated AREG stability and alters EMT to inhibit pancreatic cancer invasion and metastasis
Authors: Su Xiaowen , Lai Tiantian , Tao Yue , Zhang Yong , Zhao Changyong , Zhou Junjing , Chen Enhong , Zhu Maoqun , Zhang Shuo , Wang Bei , Mao Yong , Hu Hao 
Published: March 2023

Abstract
Background: Recent studies have shown that amphoteric regulatory protein (AREG), a member of the epidermal growth factor (EGF) family, is expressed in many cancers and is an independent prognostic indicator for patients with pancreatic cancer, but whether AREG is regulated at the epigenetic level to promote the development of pancreatic cancer (PC) has not been elucidated.
Methods: The expression level and role of AREG in PC cell tissues were investigated by GEPIA database, quantitative PCR (qPCR), Western blotting, CCK8 and Transwell assays. Methylated RNA immunoprecipitation (MeRIP) sequencing and RNA sequencing indicated that METTL3 regulates the m6A modification of AREG and affects its expression. RIP, MeRIP were used to explore its regulatory role and potential mechanisms. The association between AREG expression and clinical classification parameters was analyzed by Fisher’s exact test. In addition, we predicted that miR-33a-3p regulates METTL3 expression by miRDB and RNAinter. The intrinsic mechanisms and regulatory roles were explored using dual luciferase reporter assays and rescue experiments.
Results: Our results support the notion that AREG is overexpressed in pancreatic cancer tissues and cell lines. Functionally, the deletion of AREG impedes pancreatic cancer (PC) cell proliferation, migration, and invasion. In addition, we identified and validated that methyltransferase-like 3 (METTL3) induced the m6A modification on AREG and facilitated the stability of AREG mRNA after sequencing. Additionally, we obtained experimental evidence that miR-33a-3p targets and inhibits METTL3 from taking action. Remediation experiments showed that miR-33a-3p inhibits PC progression through METTL3.
Conclusions: this research reveals that miR-33a-3p inhibits m6A-induced stabilization of AREG by targeting METTL3, which plays a key role in the aggressive progression of PC. AREG could be a potential target for PC treatment.
Development and characterization of microsatellite markers for population genetics of the cocoa pod borer Conopomorpha cramerella (Snellen) (Lepidoptera: Gracillaridae)
Authors:   Marynold Purificacion1, Shah Roslina Binti Mohd , De Meeûs Thierry , Bakar Saripah Binti , Savantil Anisah Bintil , Yusof Meriam Mohd , Amalin Divina , Nguyen Hien , Sulistyowati Endang , Budiman Aris , Ekayanti Arni , Niogret Jerome , Ravel Sophie , Vreysen Marc J.B. , Abdalla Adly 
Published: March 2023

Abstract

The cocoa pod borer (CPB)Conopomorpha cramerella(Snellen) (Lepidoptera: Gracillaridae) is one of the major constraints for cocoa production in South East Asia. In addition to cultural and chemical control methods, autocidal control tactics such as the Sterile Insect Technique (SIT) could be a efficient addition to the currently applied control tools for CPB. The SIT is species-specific, and its successful implementation will mainly depend on adequate competitiveness of the sterile males released in the target area. In addition the technique requires an area-wide integrated pest management approach, that would target an entire pest population. Population genetic studies have in the last decades been used to provide information on gene flow between neighbouring insect populations. However, genetic markers would be required for such studies and these were not available for CPB. The aim of the present work was to search for suitable microsatellite loci in the genome of CPB that is partially sequenced. A total of 28,730 primer pairs were found and 192 pairs were selected based on the amplicon size (180-300 nt) and the motif repeats (≥11). They were synthesized and tested against the DNA ofC. cramerella. Twelve microsatellites fulfilled all parameters and these were used to analyze moths collected from Indonesia, Malaysia, and the Philippines These loci were also tested against a closely related species, i.e. the lychee fruit borerConopomorpha sinensis(LFB) from Vietnam. Genetic divergence proved substantial and one diagnostic locus could be characterized. This allowed excluding one CPB individual from Vietnam. A quality control verification process was carried out for both species and 7 microsatellites seemed to be suitable for CPB populations and 11 loci for LFB. The availability of these novel microsatellite loci will provide useful tools for the analysis of the population genetics of CPB, to understand its dynamics and ecology and to assess the level of gene flow between neighbouring populations that would be targeted with an area-wide integrated pest management approach with an SIT component.
A novel rule-based intrusion detection framework for secure Wireless Sensor Networks
Authors: Devi Manu , Nandal Priyanka , Sehrawat Harkesh 
Published: March 2023

Abstract
In order to safeguard “Wireless Sensor Networks (WSNs),” this research suggests a new rule-based intrusion detection architecture that includes a number of security techniques. The framework employs a set of rules generated by the Random Forest algorithm, which outperforms other machine learning algorithms in terms of detection accuracy, false positive rate, and overhead. The generated rules are based on features extracted from WSNs data, including packet size, energy consumption, and hop count. The framework’s performance is evaluated using a set of metrics, demonstrating its effectiveness in identifying numerous attack kinds. Combining rule-based systems and machine learning approaches in this proposed framework can improve the accuracy and efficiency of intrusion detection mechanisms for secure WSNs. The proposed framework can be a valuable addition to the existing intrusion detection mechanisms for secure WSNs.
An Efficient Hybrid Fuzzy Image Encryption Models for the Secured Cloud Accessing in Portable Robotics Devices
Authors: Reddy K Subba , Prasad K. Rajendra , Reddy K. Nageswara , Anjaiah P. 
Published: March 2023

Abstract
Secure sharing of image data through different communication channels is a challenging research issue in digital networks. In such cases, cryptography algorithms are widely used to encrypt and decrypt the information reliably at both ends of source and target destinations. Various state-of-the-art image encryption algorithms are surveyed and investigated to assess the best image encryption. In this paper, image encryption is described with various fuzzy operation modes. It ensures secure image transmission over to the digital channels. Most reliable image encryption is the aim of the work, and it is achieved through developing a hybrid fuzzy based framework that composites the techniques of image encryption methods and fuzzy operation modes. Proposed hybrid fuzzy based models are highly recommended to maintain secure information on portable robotics devices, communication channels, and routing devices. These hybrid encrypted models provide the users' privacy to access the image data with reliable security mechanisms.
Network analysis of suicide ideation and depressive-anxiety symptoms among adolescents
Authors: Xu Shuyin , Ju Yumeng , Wei Xiyu , Ou Wenwen , Ma Mohan , Lv Guanyi , Zhao Xiaotian , Qin Yaqi , Li Yunjing , Li Liang , Huang Mei , Yang Siqi , Lu Yimei , Chen Yafei , Liu Junwu , Liu Jin , Liu Bangshan , Zhang Yan 
Published: March 2023

Abstract
Background: The co-occurrence of depression and anxiety in adolescents is common and associated with suicide ideation. This study aimed to investigate the symptom-level relationship between suicide ideation and comorbidity of depression and anxiety.
Methods: R was used for network analysis of depressive and anxiety symptoms from 716 adolescents (PHQ-9 ≥ 5 and GAD-7 ≥ 5). Participants were grouped into the suicide ideation group and the non-suicide ideation group. Network differences were compared between these two groups.
Results: “Restlessness”, “Sad mood”, and “Trouble relaxing” were the most prominent central symptoms in this depressive-anxiety symptom network. “Restlessness”, “Nervousness”, and “Motor” were the bridge symptoms in this network. “Sad Mood” directly related to “Suicide Ideation” and explained the highest variance. The network structure was significantly different in network properties between adolescents who had suicide ideation and those who did not. “Restlessness” was the most central symptom in adolescents with suicide ideation. “Sad Mood” was the central and bridge symptom in adolescents with suicide ideation.
Conclusion: “Restlessness” and “Sad Mood” might be interesting avenues for reducing depression and anxiety symptoms in adolescents with suicide ideation.
Trial registration: The study received ethical approval from the Second Xiangya Hospital of Central South University on March 31, 2022.The number of Institutional Review Boards approval was 004.
Decomposition and nitrogen dynamics in vegetable crop residues with contrasting biochemical composition
Authors: Peterson Michelle , Curtin Denis , Fraser Patricia , Qiu Weiwen 
Published: March 2023

Abstract

Aim To improve nitrogen (N) use efficiency in vegetable-growing systems, the characteristics of N-release from vegetable crop residues needs to be better understood. This study aimed to quantify the decomposition rate and the contribution of decomposition to mineral N supply from residues of commonly grown vegetable crops. Methods The rate of decomposition and N release from nine vegetable residues was quantified in a laboratory incubation with barley straw included for comparison. Dried and ground residue material was incorporated into soil at a rate of 1% w/w and incubated at 90% of field capacity for 118 days at 15°C. CO2production and soil mineral N concentrations were determined periodically during the incubation. Results Decomposition of the vegetable residues was rapid, with 29–46% of residue-C respired as CO2in the first week of incubation (vs 10% of barley straw C). The decomposition data fitted an exponential decay model with evidence of fast and slow pools of decomposable-C in all residues. The effect of residue addition to soil ranged from N immobilisation to net N mineralisation over the course of the incubation with the proportion of N released or immobilised linearly related to residue total N. Conclusions With residue total N and residue-soil contact time as input variables, a simple model provided good predictions of N release from vegetable crop residues under controlled conditions in the laboratory. It is important however, that our predicted residue N release rates be validated under field conditions before being used to guide N use decisions.
Complexity of (arc)-connectivity problems involving arc-reversals or deorientations
Authors: Bang-Jensen Jørgen , Hörsch Florian , Kriesell Matthias 
Published: March 2023

Abstract
By a well known theorem of Robbins, a graph G has a strongly connected orientation if and only if G is 2-edge-connected and it is easy to find, in linear time, either a cut edge of G or a strong orientation of G. A result of Durand de Gevigny shows that for every k ≥ 3 it is NP-hard to decide if a given graph G has a k-strong orientation. Thomassen showed that one can check in polynomial time whether a given graph has a 2-strong orientation. This implies that for a given digraph D we can determine in polynomial time whether we can reorient (=reverse) some arcs of D = (V,A) to obtain a 2-strong digraph D' = (V,A'). This naturally leads to the question of determining the minimum number of such arcs to reverse before the resulting graph is 2-strong. In this paper we show that finding this number is NP-hard. If a 2-connected graph G has no 2-strong orientation, we may ask how many of its edges we may orient so that the resulting mixed graph is still 2-strong. Similarly, we may ask for a 2-edge-connected graph G how many of its edges we can orient such that the resulting mixed graph remains 2-arc-strong. We prove that when restricted to graphs satisfying suitable connectivity conditions, both of these problems are equivalent to finding the minimum number of edges we must double in a 2-edge-connected graph in order to obtain a 4-edge-connected graph. Using this, we show that all these three problems are NP-hard. Finally, we consider the operation of deorienting an arc uv of a digraph D meaning replacing it by an undirected edge between the same vertices. In terms of connectivity properties, this is equivalent to adding the opposite arc vu to D. We prove that for every ℓ ≥ 3 it is NP-hard to find the minimum number of arcs to deorient in a digraph D in order to obtain an ℓ-strong digraph D'.
Oblique stagnation point flow of magnetized Maxwell fluid over a stretchable Riga plate with Cattaneo-Christov heat flux and convective conditions
Authors: Naveed Mirza , Salamat Nadeem , Akhtar Salman , Nadeem Sohail 
Published: March 2023

Abstract
The main intension of the current work is to scrutinize the oblique stagnation point flow phenomenon of a rate-type non-Newtonian Maxwell fluid with the involvement of the Cattaneo-Christov double diffusion theory. The modified form of Fourier’s and Fick’s laws is utilized to illustrate the Cattaneo-Christov theory. The steady magnetized flow mechanism is observed in two dimensions through a stretchable convective Riga plate. In the heat and mass transfer analysis, the consequences of chemical reactions and thermal radiation are also incorporated. With the contribution of relevant dimensionless quantities, the setup of dimensionless equations is acquired which further takes the form of nonlinear equations. The physical significance of the numerous parameters on different features of the flow phenomenon is graphically exhibited. The physical quantities of interest are computed and numerically evaluated subject to the pertinent parameters. The current analysis exhibit that the Deborah number minimizes the flow field of both tangential and axial velocities. The thermal relaxation time parameter lowers the heat transfer rate, and the thermal Biot number enhances the rate of heat transport.
Evaluation of the Quantity of Extruded Debris through the Apical Foramen Using Optimum Glide Path Motion: An ex vivo study
Authors: Büker Mine , Ali Afzal , Kaya Şükriye Türkoğlu , Arslan Hakan 
Published: March 2023

Abstract
Objectives: The study was evaluated apically extruded debris from teeth that were instrumented with full-sequenced files using a new kinematic technique (optimum glide path motion).
Materials and Methods: The specimens were divided into four groups (n = 18) based on the kinematics of rotation motion (RM), 90° optimum glide path (OGP) motion, 90° clockwise (CW)–90° counterclockwise (CCW) motion, and 90° CW–120° CCW motion. Pre-weighed Eppendorf tubes were used to collect debris. The weight of the dry extruded debris was calculated by deducting the initial tube weight from the end weight. The Kruskal–Wallis test and the least significant difference for pairwise comparisons test were used to statistically assess the data (P < 0.05).
Results: In comparison to the RM, the 90° OGP motion extruded the least quantity of debris, which was statistically significant (P < 0.05).
Conclusions:The findings suggest that a 90° OGP motion delivers the best results regarding extruded debris through the apical foramen.
Clinical Relevance: Within the limitations of the study, Optimum glide path (OGP) motion resulted in less debris extrusion compared to the rotation motion. Clinical application of full-sequenced rotary systems in OGP motion may be reliable and beneficial.