bluuebunny/crossref_metadata_2025_split huggingface dataset:
Quadriceps autograft is a viable graft choice for arthroscopic ACL reconstruction in patients over 50 years of age
Authors: Meena Amit , Farinelli Luca , Hoser Christian , Abermann Elisabeth , Raj Akshya , Hepperger Caroline , Herbort Mirco , Fink Christian 
Published: March 2023

Abstract
Purpose
The purpose of this study was to evaluate the patient-reported outcomes, graft failure, quadriceps rupture and sports preference after arthroscopic ACL reconstruction in patients older than 50 years who underwent arthroscopic ACL reconstruction with a quadriceps tendon (QT) autograft.

Methods
Between 2010 and 2020, prospectively collected data were obtained from an institutional database. Patients older than 50 years with primary arthroscopic ACL reconstruction and a minimum of 2 years of follow-up were included. Patients undergoing a revision ACL reconstruction or undergoing a primary ACL reconstruction using a graft other than a QT autograft, and patients with a contralateral knee injury or osteoarthritis (Ahlbäck stage 2 or higher) were excluded. A minimally invasive technique was used for QT autograft harvesting. Patients were evaluated for pre-injury and 2-year follow-up Lysholm knee score, Tegner activity level, Visual Analog Scale (VAS) for pain, graft failure, quadriceps tendon rupture, and return to sport.

Results
A total of 57 patients were included in the study. The mean age of the cohort was 54.9 ± 5.2 (range 50–75). Of the 57 reconstructions, 16 (28%) were isolated ACL reconstructions, while 41 (72%) were complex reconstructions (concomitant meniscus, cartilage and/or collateral ligament injuries). At the 2-year follow-up Lysholm knee score, Tegner activity level and VAS for pain improved to pre-injury level and no significant difference was noted between pre-injury and 2-year follow-up functional scores (n.s.). No case of graft failure or quadriceps tendon rupture was reported. No significant difference was noted in the pre-injury and postoperative sports preference (n.s.) and all patients return to their desired sports activity.

Conclusion
Arthroscopic ACL reconstruction by using QT autograft in highly active older patients provides satisfactory patient-reported functional outcomes and allows recovery of the pre-injury level of activity. QT autograft is a good graft option in patients older than 50 years.

Level of evidence
Level IV.
Affinity-based electrochemical sensors for biomolecular detection in whole blood
Authors: Wilkirson Elizabeth C. , Singampalli Kavya L. , Li Jiran , Dixit Desh Deepak , Jiang Xue , Gonzalez Diego H. , Lillehoj Peter B. 
Published: March 2023

AbstractThe detection and/or quantification of biomarkers in blood is important for the early detection, diagnosis, and treatment of a variety of diseases and medical conditions. Among the different types of sensors for detecting molecular biomarkers, such as proteins, nucleic acids, and small-molecule drugs, affinity-based electrochemical sensors offer the advantages of high analytical sensitivity and specificity, fast detection times, simple operation, and portability. However, biomolecular detection in whole blood is challenging due to its highly complex matrix, necessitating sample purification (i.e., centrifugation), which involves the use of bulky, expensive equipment and tedious sample-handling procedures. To address these challenges, various strategies have been employed, such as purifying the blood sample directly on the sensor, employing micro-/nanoparticles to enhance the detection signal, and coating the electrode surface with blocking agents to reduce nonspecific binding, to improve the analytical performance of affinity-based electrochemical sensors without requiring sample pre-processing steps or laboratory equipment. In this article, we present an overview of affinity-based electrochemical sensor technologies that employ these strategies for biomolecular detection in whole blood.
Graphical abstract
The effect of elastic and viscous force fields on bimanual coordination
Authors: Kaur Jaskanwaljeet , Proksch Shannon , Balasubramaniam Ramesh 
Published: March 2023

AbstractBimanual in-phase and anti-phase coordination modes represent two basic movement patterns with distinct characteristics—homologous muscle contraction and non-homologous muscle contraction, respectively. A method to understand the contribution of each limb to the overall coordination pattern involves detuning (Δω) the natural eigenfrequency of each limb. In the present experiment, we experimentally broke the symmetry between the two upper limbs by adding elastic and viscous force fields using a Kinarm robot exoskeleton. We measured the effect of this symmetry breaking on coordination stability as participants performed bimanual in-phase and anti-phase movements using their left and right hand in 1:1 frequency locking mode. Differences between uncoupled frequencies were manipulated via the application of viscous & elastic force fields and using fast and slow oscillation frequencies with a custom task developed using the Kinarm robotic exoskeleton. The effects of manipulating the asymmetry between the limbs were measured through the mean and variability of relative phase (ϕ) from the intended modes of 0 ° or 180 °. In general, participants deviated less from intended phase irrespective of coordination mode in all matched conditions, except for when elastic loads are applied to both arms in the anti-phase coordination. Second, we found that when force fields were mismatched participants exhibited a larger deviation from the intended phase. Overall, there was increased phase deviation during anti-phase coordination. Finally, participants exhibited higher variability in relative phase in mismatched force conditions compared to matched force conditions, with overall higher variability during anti-phase coordination mode. We extend previous research by demonstrating that symmetry breaking caused by force differences between the limbs disrupts stability in each coordination mode.
Simple, fast and inexpensive quantification of glycolate in the urine of patients with primary hyperoxaluria type 1
Authors: Boehm Thomas , Martin-Higueras Cristina , Friesser Eva , Zitta Clara , Wallner Silvia , Walli Adam , Kovacevic Katarina , Hubmann Holger , Klavins Kristaps , Macheroux Peter , Hoppe Bernd , Jilma Bernd 
Published: March 2023

AbstractIn primary hyperoxaluria type 1 excessive endogenous production of oxalate and glycolate leads to increased urinary excretion of these metabolites. Although genetic testing is the most definitive and preferred diagnostic method, quantification of these metabolites is important for the diagnosis and evaluation of potential therapeutic interventions. Current metabolite quantification methods use laborious, technically highly complex and expensive liquid, gas or ion chromatography tandem mass spectrometry, which are available only in selected laboratories worldwide. Incubation of ortho-aminobenzaldehyde (oABA) with glyoxylate generated from glycolate using recombinant mouse glycolate oxidase (GO) and glycine leads to the formation of a stable dihydroquinazoline double aromatic ring chromophore with specific peak absorption at 440 nm. The urinary limit of detection and estimated limit of quantification derived from eight standard curves were 14.3 and 28.7 µmol glycolate per mmol creatinine, respectively. High concentrations of oxalate, lactate and L-glycerate do not interfere in this assay format. The correlation coefficient between the absorption and an ion chromatography tandem mass spectrometry method is 93% with a p value < 0.00001. The Bland–Altmann plot indicates acceptable agreement between the two methods. The glycolate quantification method using conversion of glycolate via recombinant mouse GO and fusion of oABA and glycine with glyoxylate is fast, simple, robust and inexpensive. Furthermore this method might be readily implemented into routine clinical diagnostic laboratories for glycolate measurements in primary hyperoxaluria type 1.
CIRSE Standards of Practice on Oesophageal and Gastroduodenal Stenting
Authors: Diamantopoulos Athanasios , Choudhury Shuvro Roy , Irani Farah Gillian , Tinto Hugo Rio , Sabharwal Tarun 
Published: March 2023

Abstract
Background
Image-guided insertion of stents in the upper gastrointestinal trunk is an effective, minimally invasive treatment option to provide immediate relief of symptoms caused by upper gastrointestinal tract obstruction related to advanced-stage malignant causes or benign causes that lead to lumen narrowing.

Purpose
This document, as with all CIRSE Standards of Practice documents, is not intended to impose a standard of clinical patient care but will recommend a reasonable approach to best practices for performing stenting of the upper gastrointestinal tract, namely the oesophageal and gastroduodenal segments. Our purpose is to provide up-to-date recommendations for placement of upper gastrointestinal tract stents based on the previously published guidelines on this topic in 2005 and 2007.

Methods
The writing group was established by the CIRSE Standards of Practice Committee and consisted of a group of internationally recognised experts in performing upper gastrointestinal stenting. The writing group reviewed the existing literature using PubMed to search for relevant publications in the English language up to September 2021. The final recommendations were formulated through consensus.

Conclusion
Insertion of stents in the oesophageal and gastroduodenal tracts has an established role in the successful management of malignant or benign obstructions. This Standards of Practice document provides up-to-date recommendations for the safe performance of upper gastrointestinal stent placement.
Analysis of durum wheat photosynthetic organs during grain filling reveals the ear as a water stress-tolerant organ and the peduncle as the largest pool of primary metabolites
Authors: Martínez-Peña Raquel , Vergara-Díaz Omar , Schlereth Armin , Höhne Melanie , Morcuende Rosa , Nieto-Taladriz María Teresa , Araus José Luis , Aparicio Nieves , Vicente Rubén 
Published: March 2023

Abstract
Main conclusion
The pool of carbon- and nitrogen-rich metabolites is quantitatively relevant in non-foliar photosynthetic organs during grain filling, which have a better response to water limitation than flag leaves.

Abstract
The response of durum wheat to contrasting water regimes has been extensively studied at leaf and agronomic level in previous studies, but the water stress effects on source–sink dynamics, particularly non-foliar photosynthetic organs, is more limited. Our study aims to investigate the response of different photosynthetic organs to water stress and to quantify the pool of carbon and nitrogen metabolites available for grain filling. Five durum wheat varieties were grown in field trials in the Spanish region of Castile and León under irrigated and rainfed conditions. Water stress led to a significant decrease in yield, biomass, and carbon and nitrogen assimilation, improved water use efficiency, and modified grain quality traits in the five varieties. The pool of carbon (glucose, glucose-6-phosphate, fructose, sucrose, starch, and malate) and nitrogen (glutamate, amino acids, proteins and chlorophylls) metabolites in leaf blades and sheaths, peduncles, awns, glumes and lemmas were also analysed. The results showed that the metabolism of the blades and peduncles was the most susceptible to water stress, while ear metabolism showed higher stability, particularly at mid-grain filling. Interestingly, the total metabolite content per organ highlighted that a large source of nutrients, which may be directly involved in grain filling, are found outside the blades, with the peduncles being quantitatively the most relevant. We conclude that yield improvements in our Mediterranean agro-ecosystem are highly linked to the success of shoots in producing ears and a higher number of grains, while grain filling is highly dependent on the capacity of non-foliar organs to fix CO2 and N. The ear organs show higher stress resilience than other organs, which deserves our attention in future breeding programmes.
Toxoplasmosis and cytomegalovirus infection and their role in Egyptian autistic children
Authors: Hassan Zeinab R. , Zekry Kareman M. , Heikal Elham Adel , Ibrahim Hanan F. , Khirala Seham K. , Abd El-Hamid Samar M. , Amin Doaa R. , Seliem Nora , El-Aal Gehad N. Abd , Alkherkhisy Mohammad M. , Elhamid Salwa A. Abd , Mahgoub Emad A. , Hefny Mahmoud E. N. , El Nady Ghada H. , Badr Mohamed S. 
Published: March 2023

Abstract
Autism is a neurodevelopmental disorder with a significantly increased incidence rate across the world over the past few years. Toxoplasmosis and cytomegalovirus (CMV) infection are globally prevalent and have been associated with diverse neurological and psychiatric disorders. A few studies have demonstrated the role of toxoplasmosis and CMV as potential etiological factors for autism. Accordingly, this study was performed to estimate the relationship between toxoplasmosis and CMV infection in children with autism as well as to assess their impact on the Childhood Autism Rating Scale (CARS) score. A total of 45 autistic children (6 girls, 39 boys) and 45 (21 girls, 24 boys) healthy control children were enrolled in our study. Their blood samples were collected and tested for the presence of Toxoplasma and CMV (IgG and IgM) antibodies and DNA by ELISA and real-time PCR (RT-PCR), respectively. Toxoplasmosis was detected in 11 (24.4%) autistic children through the ELISA [10 (22.2%) IgG + /IgM − and 1 (2.2%) IgG + /IgM +]; however, RT-PCR assay recorded only 1 positive case (2.2%), while it was detected in 10 (22.2%) control children through ELISA [9 (20%) IgG + /IgM − and 1 (2.2%) IgG + /IgM +] and 1 (2.2%) by RT-PCR. On the other hand, CMV infection was detected in all autistic children with 44 (97.8%) testing positive by ELISA [24 (53.3%) IgG + /IgM − , 18 (40%) IgG + /IgM + and 2 (4.4%) IgG − /IgM +] and 25 (55.6%) testing positive by RT-PCR assay. In addition, ELISA assay recorded 43 (95.6%) [19 (42.2%) IgG + /IgM + and 22 (48.9%) IgG + /IgM − and 2 (4.4%) IgG-/IgM +] and RT-PCR recorded 21 (46.7%) positive samples in control children with CMV. No significant difference was noted between autistic and control children for the overall prevalence of Toxoplasma or/and CMV infection. Similarly, the CARS score indicated a non-significant difference with Toxoplasma or/and CMV infection. Our data does not show an association between autism and toxoplasmosis or/and CMV infection. Nevertheless, considering that autistic children are at a high risk of contracting these infections, further studies with a larger sample size are recommended.
Antithrombotic agent usage before ictus in aneurysmal subarachnoid hemorrhage: relation to hemorrhage severity, clinical course, and outcome
Authors: Kultanen Hanna , Lewén Anders , Ronne-Engström Elisabeth , Enblad Per , Svedung Wettervik Teodor 
Published: March 2023

Abstract
Background
The number of patients with aneurysmal subarachnoid hemorrhage (aSAH) who are on antithrombotic agents before ictus is rising. However, their effect on early brain injury and disease development remains unclear. The primary aim of this study was to determine if antithrombotic agents (antiplatelets and anticoagulants) were associated with a worse initial hemorrhage severity, rebleeding rate, clinical course, and functional recovery after aSAH.

Methods
In this observational study, those 888 patients with aSAH, treated at the neurosurgical department, Uppsala University Hospital, between 2008 and 2018 were included. Demographic, clinical, radiological (Fisher and Hijdra score), and outcome (Extended Glasgow Outcome Scale one year post-ictus) variables were assessed.

Results
Out of 888 aSAH patients, 14% were treated with antithrombotic agents before ictus. Seventy-five percent of these were on single therapy of antiplatelets, 23% on single therapy of anticoagulants, and 3% on a combination of antithrombotic agents. Those with antithrombotic agents pre-ictus were significantly older and exhibited more co-morbidities and a worse coagulation status according to lab tests. Antithrombotic agents, both as one group and as subtypes (antiplatelets and anticoagulants), were not associated with hemorrhage severity (Hijdra score/Fisher) nor rebleeding rate. The clinical course did not differ in terms of delayed ischemic neurological deficits or last-tier treatment with thiopental and decompressive craniectomy. These patients experienced a higher mortality and lower rate of favorable outcome in univariate analyses, but this did not hold true in multiple logistic regression analyses after adjustment for age and co-morbidities.

Conclusions
After adjustment for age and co-morbidities, antithrombotic agents before aSAH ictus were not associated with worse hemorrhage severity, rebleeding rate, clinical course, or long-term functional recovery.
Autism Spectrum Disorder and auditory sensory alterations: a systematic review on the integrity of cognitive and neuronal functions related to auditory processing
Authors: Gonçalves Ana Margarida , Monteiro Patricia 
Published: March 2023

AbstractAutism Spectrum Disorder (ASD) is a neurodevelopmental condition with a wide spectrum of symptoms, mainly characterized by social, communication, and cognitive impairments. Latest diagnostic criteria according to DSM-5 (Diagnostic and Statistical Manual of Mental Disorders, Fifth Edition, 2013) now include sensory issues among the four restricted/repetitive behavior features defined as “hyper- or hypo-reactivity to sensory input or unusual interest in sensory aspects of environment”. Here, we review auditory sensory alterations in patients with ASD. Considering the updated diagnostic criteria for ASD, we examined research evidence (2015–2022) of the integrity of the cognitive function in auditory-related tasks, the integrity of the peripheral auditory system, and the integrity of the central nervous system in patients diagnosed with ASD. Taking into account the different approaches and experimental study designs, we reappraise the knowledge on auditory sensory alterations and reflect on how these might be linked with behavior symptomatology in ASD.
CH-Bench: a user-oriented benchmark for systems for efficient distant reading (design, performance, and insights)
Authors: Willkomm Jens , Raster Markus , Schäler Martin , Böhm Klemens 
Published: March 2023

AbstractData science deals with the discovery of information from large volumes of data. The data studied by scientists in the humanities include large textual corpora. An important objective is to study the ideas and expectations of a society regarding specific concepts, like “freedom” or “democracy,” both for today’s society and even more for societies of the past. Studying the meaning of words using large corpora requires efficient systems for text analysis, so-called distant reading systems. Making such systems efficient calls for a specification of the necessary functionality and clear expectations regarding typical work loads. But this currently is unclear, and there is no benchmark to evaluate distant reading systems. In this article, we propose such a benchmark, with the following innovations: As a first step, we collect and structure various information needs of the target users. We then formalize the notion of word context to facilitate the analysis of specific concepts. Using this notion, we formulate queries in line with the information needs of users. Finally, based on this, we propose concrete benchmark queries. To demonstrate the benefit of our benchmark, we conduct an evaluation, with two objectives. First, we aim at insights regarding the content of different corpora, i.e., whether and how their size and nature (e.g., popular and broad literature or specific expert literature) affect results. Second, we benchmark different data management technologies. This has allowed us to identify performance bottlenecks.
Drought Timing Modulates Soil Moisture Thresholds for CO2 Fluxes and Vegetation Responses in an Experimental Alpine Grassland
Authors: Forte T’ai G. W. , Carbognani Michele , Chiari Giorgio , Petraglia Alessandro 
Published: March 2023

AbstractDrought timing determines the degree to which dry events impact ecosystems, with the ability of key processes to withstand change differing between drought periods. Findings indicate that drought timing effects vary across ecosystems, with few studies focusing on alpine grasslands. We conducted a mesocosm experiment using small grassland monoliths collected in September from the high Alps and left to overwinter at 0 °C until the experiment began in lowland Italy under late-winter outdoor conditions. Together with watered controls, we imposed three different drought treatments (zero precipitation): (1) one-month early-drought immediately after simulated snowmelt; (2) one-month mid-drought a month after melt-out; and (3) continuous two-month drought across the entire experimental period. Ecosystem responses were assessed by measuring CO2 fluxes, while vegetation responses were investigated by measuring aboveground net primary production (ANPP) of graminoids and forbs and post-harvest resprouting after one-month rehydration. We found that ecosystem respiration and gross ecosystem production (GEP) during the day were more negatively affected by mid-season drought compared to drought starting early in the season. By the end of treatments, GEP reduction under mid-season drought was similar to that of a continuous two-month drought. ANPP reduction was similar in early- and mid-drought treatments, showing a greater decrease under an enforced two-month period without precipitation. Plant resprouting, however, was only reduced in full- and mid-season drought pots, with forbs more negatively affected than graminoids. Seasonal soil moisture variation can account for these patterns: remaining winter moisture allowed almost full canopy development during the first month of the season, despite precipitation being withheld, while soil moisture depletion in the second month, resulting from higher temperatures and greater biomass, caused a collapse of gas exchange and diminished plant resprouting. Our data illustrates the importance of the timing of zero-precipitation periods for both plant and ecosystem responses in alpine grasslands.
MicroRNA Profiling of Self-Renewing Human Neural Stem Cells Reveals Novel Sets of Differentially Expressed microRNAs During Neural Differentiation In Vitro
Authors: Fedorova Veronika , Amruz Cerna Katerina , Oppelt Jan , Pospisilova Veronika , Barta Tomas , Mraz Marek , Bohaciakova Dasa 
Published: March 2023

Abstract
The involvement of microRNAs (miRNAs) in orchestrating self-renewal and differentiation of stem cells has been revealed in a number of recent studies. And while in human pluripotent stem cells, miRNAs have been directly linked to the core pluripotency network, including the cell cycle regulation and the maintenance of the self-renewing capacity, their role in the onset of differentiation in other contexts, such as determination of neural cell fate, remains poorly described. To bridge this gap, we used three model cell types to study miRNA expression patterns: human embryonic stem cells (hESCs), hESCs-derived self-renewing neural stem cells (NSCs), and differentiating NSCs. The comprehensive miRNA profiling presented here reveals novel sets of miRNAs differentially expressed during human neural cell fate determination in vitro. Furthermore, we report a miRNA expression profile of self-renewing human NSCs, which has been lacking to this date. Our data also indicates that miRNA clusters enriched in NSCs share the target-determining seed sequence with cell cycle regulatory miRNAs expressed in pluripotent hESCs. Lastly, our mechanistic experiments confirmed that cluster miR-17–92, one of the NSCs-enriched clusters, is directly transcriptionally regulated by transcription factor c-MYC.
Graphical Abstract