bluuebunny/crossref_metadata_2025_split huggingface dataset:
MYH10 Combines with MYH9 to Recruit USP45 by Deubiquitinating Snail and Promotes Serous Ovarian Cancer Carcinogenesis, Progression, and Cisplatin Resistance
Authors: Liu Longyang , Chen Chunlin , Liu Ping , Li Jing , Pang Zhanjun , Zhu Jiayu , Lin Zhongqiu , Zhou Haixu , Xie Yingying , Lan Tiancai , Chen Zhe‐Sheng , Zeng Zhaoyang , Fang Weiyi 
Published: March 2023

AbstractThe poor prognosis of serous ovarian cancer (SOC) is due to its high invasive capacity and cisplatin resistance of SOC cells, whereas the molecular mechanisms remain poorly understood. In the present study, the expression and function of non‐muscle myosin heavy chain IIB (MYH10) in SOC are identified by immunohistochemistry, in vitro, and in vivo studies, respectively. The mechanism of MYH10 is demonstrated by co‐immunoprecipitation, GST pull‐down, confocal laser assays, and so on. The results show that the knockdown of MYH10 suppressed SOC cell proliferation, migration, invasion, metastasis, and cisplatin resistance both in vivo and in vitro. Further studies confirm that the MYH10 protein functional domain combines with non‐muscle myosin heavy chain IIA (MYH9) to recruit the deubiquitinating enzyme Ubiquitin‐specific proteases 45 and deubiquitinates snail to inhibit snail degradation, eventually promoting tumorigenesis, progression, and cisplatin resistance in SOC. In clinical samples, MYH10 expression is significantly elevated in SOC samples compared to the paratumor samples. And the expression of MYH10 is positively correlated with MYH9 expression. MYH10+/MYH9+ co‐expression is an independent prognostic factor for predicting SOC patient survival. These findings uncover a key role of the MYH10‐MYH9‐snail axis in SOC carcinogenesis, progression, and cisplatin resistance, and provide potential novel therapeutic targets for SOC intervention.
Abnormal ear development in corn: Does hybrid, environment, and seeding rate matter?
Authors: Ortez Osler A. , McMechan Anthony J. , Robinson Emily , Hoegemeyer Thomas , Howard Reka , Elmore Roger W. 
Published: March 2023

AbstractCorn (Zea mays L.) yields have increased in the United States since the 1930s and in other parts of the world since the 1950s and 1960s because of improvements in agricultural management and genotypes. Despite these increases, production concerns still exist. In July 2016, abnormal ear development (multi‐ears per node, barbell‐ears, and short‐husks) was reported in cornfields that extended from the Texas Panhandle to eastern Colorado and east through Kansas, Nebraska, Iowa, and Illinois. Surveys in Nebraska farmer fields revealed significant productivity losses due to the issues, but little was known about the underlying causes. A research study was conducted in four Nebraska fields during the 2018 and 2019 growing seasons. The research investigated the effects of hybrids, environments, seeding rates, and their interactions on abnormal ears. Eight hybrids, eight environments, and five seeding rates were studied. About 63,500 plants were individually assessed at or after the dent stage (R5). Grain yield ranged from 4.3 to 20.1 Mg ha−1. In 2018, about 5% of ears were abnormal; in 2019, about 11%, if combined, about 8%. Higher‐yielding hybrids were associated with lower percentages of abnormalities. Hybrids, environments, and seeding rates influenced the occurrence of abnormal ears. In most cases, abnormal ears had lower heights in the canopy, suggesting that primary ear loss may be a factor. The results reinforced the overriding hypothesis that ear abnormalities result from environmental, genetic, and management interactions. Depending on the environment, selecting certain hybrids with optimum seeding rates could help mitigate the occurrence of abnormal ears.
Carbon Quantum Dots with Near‐Unity Quantum Yield Bandgap Emission for Electroluminescent Light‐Emitting Diodes
Authors: Yuan Ting , Yuan Fanglong , Sui Laizhi , Zhang Yang , Li Yunchao , Li Xiaohong , Tan Zhan'ao , Fan Louzhen 
Published: March 2023

AbstractCarbon quantum dots (CQDs) feature bright and tunable photoluminescence, solution processability, and low toxicity, showing great potential in optoelectronics. However, the large‐scale synthesis of CQDs with near‐unity photoluminescence quantum yield (PLQY) has not been achieved so far. In this study, we perform radical‐assisted synthesis of hexagon‐shaped CQDs (H‐CQDs) delivering near‐unity PLQY (96 %). Experimental and theoretical analyses revealed that the large vertically oriented transition dipole moment of H‐CQDs originating from high symmetry results in nearly 100 % PLQY. The H‐CQDs also exhibited a high electron mobility of up to 0.07 cm2 V−1 s−1. These properties enable the H‐CQD‐based light‐emitting diodes with a high external quantum efficiency of 4.6 % and a record maximum brightness of over 11 000 cd m−2. This study represents a significant advance that CQDs‐based electroluminescent device can be utilized for potential display and lighting applications.
Entropic Contributions to Sodium Solvation and Solvent Stabilization upon Electrochemical Sodium Deposition from Diglyme and Propylene Carbonate Electrolytes
Authors: Karcher Franziska , Uhl Matthias , Geng Tanja , Jacob Timo , Schuster Rolf 
Published: March 2023

AbstractThe formation of an appropriate solid electrolyte interphase (SEI) at the anode of a sodium battery is crucially dependent on the electrochemical stability of solvent and electrolyte at the redox potential of Na/Na+ in the respective system. In order to determine entropic contributions to the relative stability of the electrolyte solution, we measure the reaction entropy of Na metal deposition for diglyme (DG) and propylene carbonate (PC) based electrolyte solutions by electrochemical microcalorimetry at single electrodes. We found a large positive reaction entropy for Na+ deposition in DG of ΔR
234 J mol−1 K−1 (c.f.: ΔR
83 J mol−1 K−1), which signals substantial entropic destabilization of Na+ in DG by about 0.73 eV, thus increasing the stability of solvent and electrolyte relative to Na+ reduction. We attribute this strong entropic destabilization to a highly negative solvation entropy of Na+, due to the low dielectric constant and high freezing entropy of DG.
Messung von Nanometerabständen in Lösung mittels 13C NMR‐Spektroskopie – Schrumpfen von Makrocyclen mit zunehmender Temperatur
Authors: Siera Hannah , Mehrparvar Saber , Fax Jonas , Kreuzahler Mathis , Haberhauer Gebhard 
Published: March 2023

AbstractBei molekularen Systemen sind Größe und Form von elementarer Bedeutung für Funktion und Eigenschaften. Daher ist die Bestimmung von Abständen innerhalb eines Moleküls elementar. Die aktuell gängigen Methoden sind jedoch nur für Abstände kleiner als 4 Å oder größer als 15 Å geeignet. Hier zeigen wir, dass wir durch den Einbau einer molekularen Feder Abstände in Makrocyclen im Bereich von 10 Å mit Hilfe der 13C NMR‐Spektroskopie messen können. Die Genauigkeit der Methode erlaubt es auch die Temperaturabhängigkeit der Abstände zu bestimmen. In einem Fall findet man bei Erwärmung ein Schrumpfen der Länge um fast 10 %. Diese Verkürzung durch Erwärmung kann als inverse Thermoelastizität auf molekularer Ebene betrachtet werden und ist ein bisher völlig vernachlässigtes Phänomen, das in Zukunft als Werkzeug zur Veränderung der Länge und damit der Funktion eines Systems genutzt werden kann.
Morphological Profiling Identifies the Motor Protein Eg5 as Cellular Target of Spirooxindoles
Authors: Liu Jie , Mallick Shubhadip , Xie Yusheng , Grassin Corentin , Lucas Belén , Schölermann Beate , Pahl Axel , Scheel Rebecca , Strohmann Carsten , Protzel Christoph , Berg Thorsten , Merten Christian , Ziegler Slava , Waldmann Herbert 
Published: March 2023

AbstractOxindoles and iso‐oxindoles are natural product‐derived scaffolds that provide inspiration for the design and synthesis of novel biologically relevant compound classes. Notably, the spirocyclic connection of oxindoles with iso‐oxindoles has not been explored by nature but promises to provide structurally related compounds endowed with novel bioactivity. Therefore, methods for their efficient synthesis and the conclusive discovery of their cellular targets are highly desirable. We describe a selective RhIII‐catalyzed scaffold‐divergent synthesis of spirooxindole–isooxindoles and spirooxindole–oxindoles from differently protected diazooxindoles and N‐pivaloyloxy aryl amides which includes a functional group‐controlled Lossen rearrangement as key step. Unbiased morphological profiling of a corresponding compound collection in the Cell Painting assay efficiently identified the mitotic kinesin Eg5 as the cellular target of the spirooxindoles, defining a unique Eg5 inhibitor chemotype.
Electrically Amplified Circularly Polarized Luminescence by a Chiral Anion Strategy
Authors: Li Zhong‐Qiu , Wang Yu‐Duan , Shao Jiang‐Yang , Zhou Zeyang , Gong Zhong‐Liang , Zhang Chuang , Yao Jiannian , Zhong Yu‐Wu 
Published: March 2023

AbstractThe development of circularly polarized electroluminescence (CPEL) is currently hampered by the high difficulty and cost in the syntheses of suitable chiral materials and the notorious chirality diminishment issue in electrical devices. Herein, diastereomeric IrIII and RuII complexes with chiral (±)‐camphorsulfonate counteranions are readily synthesized and used as the active materials in circularly polarized light‐emitting electrochemical cells to generate promising CPELs. The addition of the chiral ionic liquid (±)‐1‐butyl‐3‐methylimidazole camphorsulfonate into the active layer significantly improves the device performance and the electroluminescence dissymmetry factors (≈10−3), in stark contrast to the very weak circularly polarized photoluminescence of the spin‐coated films of these diastereomeric complexes. Control experiments with enantiopure IrIII complexes suggest that the chiral anions play a dominant role in the electrically‐induced amplification of CPELs.
Upcycling of Polybutadiene Facilitated by Selenium‐Mediated Allylic Amination
Authors: Hodges Mercie N. , Elardo Matthew J. , Seo Jinyoung , Dohoda Alexander F. , Michael Forrest E. , Golder Matthew R. 
Published: March 2023

AbstractAccumulation of end‐of‐life plastics presents ongoing environmental concerns. One strategy to solve this grand challenge is to invent new techniques that modify post‐consumer waste and impart new functionality. While promising approaches for the chemical upcycling of commodity polyolefins and polyaromatics exist, analogous approaches to repurpose unsaturated polymers (e.g., polybutadiene) are scarce. In this work, we propose a method to upcycle polybutadiene, one of the most widely used commercial rubbers, via a mild, metal‐free allylic amination reaction. The resulting materials have tunable thermal and surface wetting properties as a function of both sulfonamide identity and grafting density. Importantly, this approach maintains the parent alkene microstructure without evidence of olefin reduction, olefin transposition, and/or chain scission. Based on these findings, we anticipate future applications in the remediation of complex elastomers and vulcanized rubbers.
Large‐Scale and Controllable Syntheses of Covalently‐Crosslinked Poly(ionic liquid) Nanoporous Membranes
Authors: Hu Yingyi , Xu Luyao , Zhang Wangqing , Wang Hong 
Published: March 2023

AbstractHerein, we report an exciting synthetic procedure for the scalable and controllable fabrication of covalently crosslinked poly(ionic liquid) (PIL) nanoporous membranes (CPILMs) in water solution under ambient conditions. We found that the pore sizes, flexibility and compositions of freestanding CPILMs can be finely tailored by a rational structural choice of PIL, diketone and aldehyde. Studies on the CPILM formation mechanism revealed that hydrogen bonding‐induced phase separation of amino‐functionalized homo‐PIL between its polar and apolar domains coupled with structural rearrangements due to the Debus Radsizewski reaction‐triggered ambient covalent crosslinking process created a stable three‐dimensionally interconnected pore system in water solution. Employing structurally stable CPILMs in ion sieving devices resulted in an excellent Li+/Mg2+ separation efficiency due to the positively charged nature and “Donann” effects. This green, facile yet versatile approach to the production of CPILMs is a conceptually distinct and commercially interesting strategy for making useful nanoporous functional polyelectrolyte membranes.
An operation on placental support in a fetus with a nasopharyngeal teratoma
Authors: Yanko F. , Nathani H. , Alden T. , Valika T. , Rastatter J. , Alhajjat A. , Ballard H. A. 
Published: March 2023

SummaryAirway obstruction diagnosed antenatally in the fetus due to congenital high airway obstruction syndrome or nasopharyngeal masses are the primary indications for performing an operation on placental support. Prenatally diagnosed nasopharyngeal masses pose the risk of total airway obstruction upon delivery. Placental support utilises uteroplacental blood flow to facilitate an airway intervention on the fetus while maintaining oxygenation. These interventions must be completed in under 20 min due to amniotic fluid loss and uterine contractions. This case report describes the anaesthetic management of a fetus with a nasal teratoma. In this report, we discuss the clinical indications and anaesthetic considerations of the operation on placental support procedure for nasopharyngeal masses.
An image reconstruction modeling approach for micro‐fibrous network of cellulose polymer separator concerning tensile and pore properties
Authors: Liu Wangyu , Wu Lin , Dang Yanping , Gou Jingren , Xie Weigui , Tang Aimin 
Published: March 2023

AbstractCellulose is applicable for preparing lithium‐ion battery separators due to its excellent properties. Factors affecting the performance of the cellulose separator can be explored via modeling the micro‐fibrous structure, which can provide guidance for preparing design and optimization of separators. In this paper, a three‐dimensional modeling method based on digital image processing is proposed for random fibrous network of cellulose separator, with a strong focus on the fibrous parameters. Finite element simulation on elastic mechanics is conducted, and the modeling accuracy has been discussed, where the effect of the fiber diameter and quantity on the elastic property of fibrous separator is comprehensively investigated. The relationship between porosity and elastic modulus can be predicted by both modeling simulation and experiment with a small error, indicating that the modeling method is credible. The pore property, that is, porosity and tortuosity, of the constructed model is calculated and compared with related work to characterize battery performance of separator. The result has shown the feasibility of the fibrous model in pore study. This work has provided an effective and reliable modeling approach for fibrous network, which can be utilized to investigate the mechanical and pore properties of battery separators.
Pt‐Based Rare Earth Alloy as Efficient and Robust Electrocatalyst for High‐Temperature Proton Exchange Membrane Fuel Cells
Authors: Lin Jiaqi , Wang Jun , Wu Yujie , Yang Pupu , Liu Qie , Li Miaoyu , Du Shiqian , Chen Ru , Tao Li 
Published: March 2023

AbstractHigh‐temperature proton exchange membrane fuel cells (HT‐PEMFCs) are crucial in future energy systems. However, the activity and stability of the electrocatalysts are severely restricted by high temperature and phosphoric acid poisoning. Herein, PtCe alloy as oxygen reduction reaction (ORR) electrocatalyst for HT‐PEMFCs exhibits fantastic performance. Ce can increase the electronic density of Pt, weakening phosphoric acid poisoning and improving ORR activity. The optimized electronic structure can also reduce the dipole effect between Pt and O, which suppresses the irreversible oxidation of Pt. Additionally, the dramatically negative heat of formation in PtCe catalyst brings high kinetic barrier of metal diffusion and dissolution. With this electrocatalyst, the HT‐PEMFCs show a preeminent peak power of 605 mW cm−2 with 0.3 mgPt cm−2. After 30000 cycles of accelerated stability test, the peak power density only decreases by 31.6%, achieving the goal of Department of Energy in 2020 (<40% loss).
Gestational exposure to continuous light impairs the development of the female reproductive system in adult Wistar rat offspring
Authors: Ogo Fernanda Mithie , Siervo Glaucia Eloisa Munhoz Lion , Praxedes Ana Maria , Vieira Henrique Rodrigues , da Silva Scarton Suellen Ribeiro , Bitencourt Ana Tereza Guimarães , Arena Arielle Cristina , Simão Andréa Name Colado , Guerra Marina Trevizan , de Freitas Mathias Paulo Cesar , Fernandes Glaura Scantamburlo Alves 
Published: March 2023

AbstractIntroductionIt has been suggested that maternal exposure to constant light during the gestational period could be considered as a chronic stressor, impairing offspring development by interfering in neuroendocrine and behavior responses.ObjectiveThis study aimed to evaluate whether maternal exposure to continuous light during pregnancy affects the adult reproductive system in the female offspring.Materials and MethodsPregnant Wistar rats were allocated into light–dark (LD) group, exposed to light and dark photoperiod during gestation, and the light–light (LL) group, exposed to a photoperiod of constant light during gestation. After birth, pups were maintained under normal light–dark photoperiod until adulthood. At postnatal day 90, blood was collected from the female offspring, to analyze plasma luteinizing hormone (LH) and progesterone levels, and the uterus and ovaries were harvested for morphometric, histological, and oxidative stress evaluations.Results and DiscussionFemale exposure to continuous light during the intrauterine period resulted in the adult reduction of LH and increased progesterone plasma levels, and uterine injuries a higher number of endometrial glands and reduced levels of antioxidant enzymes, such as glutathione reductase and glutathione S‐transferase. In these experimental conditions, gestational continuous light exposure disturbs sex hormone balance and reduces the antioxidant enzymatic activity in the uterus of female offspring in adult life.
Crystallographic, morphological and photoluminescent investigations of Tb3+‐doped YAlO3 perovskites for lighting applications
Authors: Gupta Isha , Singh Devender , Singh Sitender , Kumar Pawan , Bhagwan Shri , Kumar Vinod , Kumar Harish , Chhikara Sunil Kumar 
Published: March 2023

AbstractTerbium(III)‐doped yttrium aluminate perovskite (YAP:xTb3+) (x = 0.01–0.08 mol) was synthesized using a simple gel‐combustion method. Structural elucidations were performed using X‐ray diffraction (XRD) and Rietveld analysis. Fourier‐transform infrared spectral studies validated the efficient synthesis of designed doped samples. Transmission electron microscopic images showed the agglomerated irregular dimensions of the synthesized nanocrystalline materials. When excited at 251 nm, a strong emissive line attributed to 5D4 → 7F5 electronic transition was observed at 545 nm (green emission). The maximum luminescence was found at the optimized concentration (0.05 mol) of Tb3+ ions; this emission was quenched by dipolar–dipolar (d–d) interactions. Chromaticity (x and y) and correlated colour temperature parameters were obtained by analysing the emission profiles. Finally, the colour coordinates of nanophosphors were closer to the National Television Standards Committee green coordinates, which replicates their potency in the design and architecture of R‐G‐B‐based white LEDs.
Trace elements as potential modulators of puberty‐induced amelioration of oxidative stress and inflammation in childhood obesity
Authors: González‐Domínguez Álvaro , Domínguez‐Riscart Jesús , Millán‐Martínez María , Mateos‐Bernal Rosa María , Lechuga‐Sancho Alfonso María , González‐Domínguez Raúl 
Published: March 2023

AbstractAlthough puberty is known to influence obesity progression, the molecular mechanisms underlying the role of sexual maturation in obesity‐related complications remains largely unexplored. Here, we delve into the impact of puberty on the most relevant pathogenic hallmarks of obesity, namely oxidative stress and inflammation, and their association with trace element blood status. To this end, we studied a well‐characterized observational cohort comprising prepubertal (N = 46) and pubertal (N = 48) children with obesity. From all participants, plasma and erythrocyte samples were collected and subjected to metallomics analysis and determination of classical biomarkers of oxidative stress and inflammation. Besides the expected raise of sexual hormones, pubertal children displayed better inflammatory and oxidative control, as reflected by lower levels of C‐reactive protein and oxidative damage markers, as well as improved antioxidant defense. This was in turn accompanied by a healthier multielemental profile, with increased levels of essential elements involved in the antioxidant system and metabolic control (metalloproteins containing zinc, molybdenum, selenium, and manganese) and decreased content of potentially deleterious species (total copper, labile free iron). Therefore, our findings suggest that children with obesity have an exacerbated inflammatory and oxidative damage at early ages, which could be ameliorated during pubertal development by the action of trace element‐mediated buffering mechanisms.
Characterization of ionic strength for X‐MuLV inactivation by low pH treatment for monoclonal antibody purification
Authors: Daya Jena , Cusick Valerie , Mattila John 
Published: March 2023

AbstractIn the production of monoclonal antibodies (mAbs) intended for use in humans, it is a global regulatory requirement that the manufacturing process includes unit operations that are proven to inactivate or remove adventitious agents to ensure viral safety. Viral inactivation by low pH hold (LPH) is typically used to ensure this viral safety in the purification process of mAbs and other biotherapeutics derived from mammalian cell lines. To ascertain the effectiveness of the LPH step, viral clearance studies have evaluated LPH under worst‐case conditions of pH above the manufacturing set point and hold duration at or below the manufacturing minimum. Highly acidic conditions (i.e., pH < 3.60) provide robust and effective enveloped virus inactivation but may lead to reduced product quality of the therapeutic protein. However, when viral inactivation is operated above pH 3.60 to ensure product stability, effective (>4 log10 reduction factor) viral inactivation may not be observed under these worst‐case pH conditions in viral clearance studies. A multivariate design of experiments was conducted to further characterize the operating space for low pH viral inactivation of a model retrovirus, xenotropic murine leukemia virus (X‐MuLV). The statistically designed experiment evaluated the effect of mAb isotype, pH, temperature, acid titrant, sodium chloride (NaCl) concentration, virus spike timing, and post‐spike filtration on X‐MuLV inactivation. Data from the characterization study were used to generate predictive models to identify conditions that reliably achieve effective viral inactivation at pH ≥ 3.60. Results of the study demonstrated that NaCl concentration has the greatest effect on virus inactivation in the range studied, and pH has a large effect when the load material has no additional NaCl. Overall, robust and effective inactivation of X‐MuLV at pH 3.65–3.80 can be achieved by manipulating either the pH or the NaCl concentration of the load material. This study contributes to the understanding of ionic strength as an influential parameter in low pH viral inactivation studies.