bluuebunny/crossref_metadata_2025_split huggingface dataset:
Clinical Features and Surgical Outcome of Clear Cell Papillary Renal Cell Tumor: Result from a Prospective Cohort
Authors: Kim Si Hyun , Han Jang Hee , Jeong Seung-hwan , Yuk Hyeong Dong , Ku Ja Hyeon , Kwak Cheol , Kim Hyeon Hoe , Moon Kyung Chul , Jeong Chang Wook 
Published: February 2023

Abstract
Background: Clear cell papillary renal cell tumor (CCPRCT) was first reported in 2006 a patient with end stage renal disease. After that it was discovered in the kidney without end stage renal disease in the 2010s and started to be mentioned in pathology and urology. The incidence of CCPRCT is low and most of it is discovered incidentally, so there is a lack of reports on clinical characteristics and surgical outcome.
Methods: This study used clinical data from the Seoul National University Prospectively Enrolled Registry for Renal Cell Carcinoma-Nephrectomy (SUPER-RCC-Nx). Between August 2016 and July 2022, patients who underwent radical or partial nephrectomy with clear cell papillary RCC with pathological finding were included in this study. All patients’ pathologic reports were reviewed by 1 pathologist. Clinical characteristics and surgical outcomes were presented through descriptive statistics, and Kaplan-Meier curve used for survival analysis.
Results: Of the 2057 patients, CCPRCT was reported in 36 patients (1.8%). The median follow up period was 26.8 months. The median age was 67 years, and there were 10 females and 26 males. The median tumor size was 1.2 cm. Twenty-nine patients underwent partial nephrectomy. Seven patients with end-stage renal disease underwent radical nephrectomy. The median operative time for patients who underwent partial nephrectomy was 97.5 minutes and the estimated blood loss was 100 cc. The median hospital days was 4 and 30-day complications were 2 cases with clavien-dindo classification III or higher. During the follow-up period, there was no recurrence and cancer specific mortality.
Conclusions: The size of CCPRCT was small and there was no advanced stage at that time of diagnosis. There was no recurrence or cancer specific mortality during the follow-up period. A multi-center study with a large scale is needed in the future.
Trial registration: Seoul National University Hospital (SNUH) Institutional Review Board (IRB) (approval number: 2210-126-1371)
A novel distributed deep learning approach for large-scale chest X-ray covid-19 images detection
Authors: MEZZOUDJ Saliha , BELKESSA Imane , BOURAS Feriel , KHELIFA Meriem 
Published: February 2023

Abstract
Recently, the high mortality rates caused by the covid-19 pandemic
have formed a crucial problem to support its enormous and rapid spread. Due to
the need for accurate and rapid diagnosis of patients, it is necessary to develop
a self-operating recognition model as a fast recognition system to detect covid-
19 infection and prevent the rapid spread among people. Chest X-ray images
have been demonstrated to be useful in detecting the covid-19 cases. Further-
more, covid-19 being a new disease, the annotated large-scale CXR dataset (big
data) for this particular disease is virtually nonexistent. We propose exploiting
a large-scale chest X-ray dataset and training a distributed deep neural network
to address the limited data issue. In this paper, we propose a new distributed
deep learning-based approach (COV-DCNN) for detecting large-scale chest X-ray
covid-19 images using ImageDataGenerator to do dynamic data preprocessing and
splitting and spark TensorFlow distributor framework to execute distributed training tasks using a Spark job in barrier mode. Furthermore, we adopted a distributed
Deep Convolutional Neural Network (DCNN), ANN, AlexNet on Spark Tensor-
Flow Distributor to classify large-scale chest X-ray covid-19 images. The highest
accuracy was obtained by the DCNN model, which is 91.88% during 0.006s for
each case, 84.62% for the ANN model, and 73.8% for the AlexNet model. The
obtained results achieve high classification accuracy at a faster speed than other
state-of-the-art classification methods
Inflammatory bowel disease perception among Chinese patients: Development and Validation of a knowledge questionnaire
Authors: xie huabing , Zhang Jixiang , Liu Chuan , Yang Bingxiang , Dong Weiguo 
Published: February 2023

Abstract
A good understanding of the disease facilitates patient-centered management. We aimed to develop and validate a new questionnaire to assess inflammatory bowel disease (IBD)-related knowledge, and to analyze the related factors affecting their knowledge of IBD. We invited 15 experts to develop and modify the IBD knowledge questionnaire, and 709 patients to test the reliability and validity of the questionnaire, and analyze the factors related to the disease knowledge of patients with IBD. Internal consistency: Cronbach's α coefficients of the total, UC, and CD knowledge questionnaire were 0.886, 0.89, and 0.886 respectively. Cross-item consistency, the Spearman-Brown split coefficients of the total, UC, and CD knowledge questionnaire were 0.843, 0.812, and 0.812 respectively. Time consistency, the test-retest reliability ICC was 0.862 (P < 0.001). The correlation R between the researcher score and the IBD-KNOW score and the original questionnaire score was greater than 0.7 (P < 0.001). Multiple linear regression demonstrated that disease type, age, body mass index (BMI), education level, income, treatment cost, duration of disease, and frequency of visits were related factors that affected IBD patients' disease knowledge (P < 0.05). The IBD Knowledge questionnaire has good reliability and validity, and can therefore be used to assess patient knowledge of the disease.
Companion crops alter olfactory responses of the fall armyworm (Spodoptera frugiperda), and its larval endoparasitoid (Cotesia icipe)
Authors: Peter Emmanuel , Tamiru Amanuel , Sevgan Subramanian , Dubois Thomas , Kelemu Segenet , Kruger Kerstin , Torto Baldwyn , Yusuf Abdullahi 
Published: February 2023

Abstract

The fall armyworm (FAW), Spodoptera frugiperda, is a devastating invasive pest and a threat to food security in Africa, with yield losses of 20–50%. Recent studies highlighted the importance of cereal crops such as maize and sorghum as the most preferred host plants for FAW oviposition. In the current work, we investigated the olfactory responses of FAW and its larval endoparasitoid Cotesia icipe to odours from the preferred host (maize) in the presence of six potential companion crops including beans, groundnut, sweet potato, greenleaf- and silverleaf desmodium, and cassava. We hypothesized that odours released by companion crops in maize-based intercropping systems would alter host preferences of FAW for oviposition and its parasitoid responses. In dual choice bioassays, FAW laid significantly more eggs on maize than on the other plants; however, significantly fewer eggs were laid on maize when companion plants were present. Markedly, the presence of cassava did not affect the oviposition responses of FAW. While wind tunnel bioassays confirmed the differential behavioural responses of the FAW, we found that its larval endoparasitoid C. icipe was attracted to volatiles from both the individual companion plants and when they were combined with maize. Coupled gas chromatography-mass spectrometry (GC-MS) analysis detected several potential behaviour-modifying compounds including (Z)-3-hexenyl acetate, (E)-β-ocimene, (E)-4,8-dimethyl-1,3,7-nonatriene, (E)-β-caryophyllene, camphor, methyl salicylate and (E, E)-4,8,12-trimethyl-1,3,7,11-tridecatetraene. Our findings provide evidence supporting diversified maize cropping system could reduce FAW damage by repelling the pest while recruiting its natural enemies and hence may serve as an ecologically sustainable FAW management strategy.
Investigation on the high feed milling of M28
Authors: Jiang Yifan , Yang Xu , Tian Hui , Ma Liangdong , Li Liang , Yang Yinfei 
Published: February 2023

Abstract
The outstanding combination of high strength, fracture toughness and low mass makes the metastable β titanium alloy an ideal material for aviation application. Machining of the metastable β titanium alloy remains a challenge due to their poor machinability. In this study, the high feed milling of a novel metastable β titanium alloy M28 is investigated to evaluate the performance of a potential high-efficiency-machining approach for the next generation titanium alloy and promote the understanding of high feed milling. The cutting force and the wear mechanism of carbide tool coated with TiAlN-Al2O3 are investigated as well as the influence of high feed on machined surface microstructures and microhardness. Due to the chip thinning effect of high feed milling, a proper high feed maintains a sufficient chip thickness to prevent the ploughing. Cutting edge degeneration induced by the carbide matrix fracture accelerates the tool wear. Mechanical stress induced cracks lead to the uneven delamination of the TiAlN-Al2O3 coating. Slight effect of high feed can be found on the microstructures and microhardness of the machined surface. While the tool wear leads to significant grain fragmentation and distortion at the surface. Since no white layer is found in the cutting of M28, high feed milling shows the advantage of preventing the heat induced surface injury.
Sex-based Differences in the Distribution of Aujeszky’s Disease-Seropositive Japanese Wild Swine
Authors: Yamaguchi Emi , Takagi Michihiro , Osaki Makoto , Hayama Yoko , Yamamoto Takehisa 
Published: February 2023

Abstract

Background: Aujeszky’s disease virus (ADV) primarily infects domestic and wild swine, causing the abortion and death of young piglets due to central nervous system disorders. In Japan, the national eradication program has been successful in most prefectures; however, ADV-infected wild swine have been concerned as a source of ADV among domestic pigs.
Results: This study assessed the nationwide seroprevalence of ADV among wild swine (Sus scrofa) in Japan. Moreover, sex-based differences in the spatial clustering of seropositive animals were investigated. In total, 1383 serum samples were obtained from wild swine caught in 41 prefectures in three fiscal years (April–March in 2014, 2015, and 2017) in Japan. Next, the seropositivity for ADV was evaluated using enzyme-linked immunosorbent assay and the latex agglutination and neutralization tests. Results showed that 29 swine were seropositive for ADV (29/1383, 2.1% [95% confidence interval, CI: 1.4%–3.0%]). Among them, 28 were caught in three prefectures located at the Kii Peninsula (28/121, 23.1% [95% CI: 16.0%–31.7%]). The degree of spatial clustering of ADV-seropositive adult swine at the Kii Peninsula according to sex was evaluated using K-function with the capture locations of 46 males including 14 seropositive and 54 females including 12 seropositive. In females, the degree of clustering was significantly higher in seropositive animals than in tested animals; however, such difference was not observed for seropositive males.
Conclusions: The spatial dynamics of ADV among adult wild swine might be characterized based on sex. This finding might be attributed to sex-based differences in behavioral patterns including dispersal among wild swine.
EBV positive inflammatory follicular dendritic cell sarcoma of colon with clonal immunoglobulin receptor gene rearrangement: a case report and literature review
Authors: Xu Xia , Li Xiuzhen , Deng Qun , Yu Kaihang , Li Jinfan 
Published: February 2023

Abstract

Background: Epstein-Barr virus positive (EBV+) inflammatory follicular dendritic cell (FDC) sarcoma is a neoplasm of spindled follicular dendritic cells with abundant lymphoplasmacyte infiltration and a consistent association with EBV. It usually involves the liver and spleen but rarely the digestive tract. Herein, we reported a special case of EBV+ inflammatory FDC sarcoma in colon with clonal immunoglobulin (IG) receptor gene rearrangement.
Case presentation: A 70-year-old man presented with abdominal distension for 1 month. A pedunculated polyp in ascending colon was revealed by colonoscopic examination. The patient then underwent endoscopic polypectomy. The colonic polyp had extremely prominent lymphoplasmacytic infiltrates with dispersed EBV+ neoplastic cells, highlighted by EBV-encoded small RNA in situ hybridization. The neoplastic cells were immunoreactive for FDC markers (CD21, CD35 and CD23). The tumor also showed an amplification of immunoglobulin heavy chain (IGH) gene rearrangement. Finally, its diagnose was EBV+ inflammatory follicular dendritic cell sarcoma.
Conclusions: We represented a rare case of EBV+ inflammatory FDC sarcoma presenting as a colonic polyp with clonal IGH gene rearrangement. The molecular change is first described in this tumor of colon. Awareness of this rare neoplasm in gastrointestinal tract is important for accurate diagnosis and well patient management.
MYC directly transactivates CR2/CD21, the receptor of the Epstein-Barr virus, enhancing the viral infection of Burkitt lymphoma cells
Authors: León Javier , Molina Ester , Garcia-Gutierrez Lucia , Junco Vanessa , Perez-Olivares Mercedes , de Yebenes Virginia G. , Blanco Rosa , Quevedo Laura , Acosta Juan C. , Marin Ana V. , Ulgiati Daniela , Merino Ramon , Varela Ignacio , Regueiro Jose R. , de Alboran Ignacio Morenos , Ramiro Almudena R. 
Published: February 2023

Abstract
MYC is an oncogenic transcription factor dysregulated in about half of total human tumors. Transcriptomic studies reveal more than 1000 genes regulated by MYC but a much smaller fraction of genes is directly transactivated by MYC. Virtually all Burkitt lymphoma (BL) carry chromosomal translocations involving MYC oncogene. All endemic BL and a fraction of sporadic BL are associated to Epstein-Barr virus (EBV) infection. The currently accepted mechanism is that EBV is the lymphoma causing agent inducing MYC translocation. Herein we show that the EBV receptor gene, CR2 (or CD21), is a bona fide direct MYC target gene. This is based on several evidences: MYC induces CR2 expression in both proliferating and arrested cells, binds the human CR2 promoter and transactivates CR2 in an E-box dependent manner and in the absence of protein synthesis. Moreover, using mice with conditional MYC ablation we show that MYC induces CR2 in primary B cells. Importantly, MYC silencing or MYC overexpression in BL cells results in less and more efficient EBV infection, respectively. Altogether, in contrast to the widely accepted mechanism for the correlation between EBV and endemic BL, we propose an alternative mechanism in which MYC dysregulation occurs first and favors the EBV infection.
Short hairpin RNA-directed LDHA silencing using liposomal nanovectors for the effective treatment of human hepatocellular carcinoma
Authors: Zhou Xiang , Chen Ruohua , Lin Chao , Huang Gang , Liu Jianjun 
Published: February 2023

Abstract
Background
Lactate dehydrogenase A (LDHA), which converts pyruvate to lactate, is over-expressed in many malignant tumors. We will analyze the correlation between LDHA expression and clinicopathological features of HCC. We further applied biodegradable polyethyleneimine containing multiple disulfide bonds (SS-PEI) as an effective non-viral vector for the safe intracellular delivery of plasmid LDHA-shRNA-EGFP in vitro and in vivo for the treatment of liver cancer.
Methods
We first retrospectively analyzed the correlation between the expression of LDHA in liver cancer and the clinical case characteristics of patients and the prognosis of anti-PD1/PD-L1 therapy. Further, I synthesized a nanomaterial SS-PEI/pLDHA-shRNA-EGFP that targets and inhibits the expression of LDHA. Through cytological and animal experiments, it is confirmed that the effectiveness and safety of inhibiting tumor growth.
Results
Our studies showed that patients with hepatocellular carcinoma (HCC) with high LDHA expression have poorer overall survival and disease-free survival. The expression of PD-L1 in HCC was positively correlated with the expression of Ki-67, PD-L1 and the infiltration of Regulatory T cells, CAF cells in the tumor microenvironment (p < 0.05). The objective response rate of anti-PD1/PD-L1 immunotherapy in HCC patients with low LDHA expression was significantly higher than patients with high LDHA expression(p < 0.05). Transfection experiments showed that the SS-PEI/pLDHA-shRNA-EGFP complexes could be used to transfect various types of HCC cell lines in vitro, inducing reduced expression of LDHA and cell growth inhibition. In addition, treatment of mice with SS-PEI/pLDHA-shRNA-EGFP by tail vein injection significantly inhibited the growth of subcutaneous xenograft tumors. Meanwhile, the complexes revealed relatively low cytotoxicity in vitro and no toxicity was observed in vivo.
Conclusions
The expression of LDHA in HCC is closely related to tumor proliferation and immune escape. Patients with low LDHA expression of HCC are more sensitive to anti-PD1/PD-L1 immunotherapy. Targeting LDHA in vivo with a specific short hairpin RNA delivered via SS-PEI is a promising therapy for patients with HCC.
Impact of Cross-linking Agents on the Performance of Alginate- Cellulose Nanocrystals Adsorbent Beads
Authors: Grishkewich Nathan , Mohammed Nishil , Tam Kam Chiu 
Published: February 2023

Abstract
A detailed investigation on the adsorption of methylene blue (MB) to sodium alginate/cellulose nanocrystal (ALG-CNC) composite hydrogel beads was conducted. The impact of physical cross-linking and calcium ions on the binding of MB onto the beads and the swelling/shrinkage of bead during the adsorption process in batch vessel and continuous fixed bed operations was examined. Changes in the bulk calcium ion concentrations during the batch adsorption experiments on the binding of MB to the beads were monitored when the beads were subjected to different pretreatments. The calcium ions reduced the adsorption capacity by charge screening instead of competitive adsorption, and the removal of calcium ions contributed to an initial shrinkage of the adsorbent during binding tests in the packed column.
An analysis of well-being in Gauteng province using the capability approach
Authors: Mushongera Darlington , Ntuli Miracle , Kwenda Prudence 
Published: February 2023

Abstract

This paper adopts a standard ‘capability approach’ to analyse individual well-being in Gauteng province of South Africa. The analysis is based on eight capabilities namely; ‘play’, ‘emotions’, ‘other species’, ‘affiliation’, ‘bodily health’, ‘bodily integrity’, ‘senses, imagination and thought’ and ‘control over one’s environment’. Data for analysis came from the Gauteng City-Region Observatory Quality of Life Survey IV-2015/16. A Multiple Correspondence Analysis was employed to generate an index for each capability. Each index is based on individual ‘functionings’ that deliberately cover both ‘hard’ and ‘soft’ indicators of well-being undergirded by assumptions of aspirations and freedom. Results show that capability achievements vary across race, age and gender, confirming the typical South African scenario of inequality. However, we observe broader levels of deprivation that are otherwise masked in earlier studies based only on objective dimensions. JEL Classification C18 I32
Inhibition of GSK3β activity alleviates acute liver failure via suppressing multiple programmed cell death
Authors: Zhang Danmei , Shi Chunxia , Zhang Qingqi , Wang Yukun , Guo Jin , Gong Zuojiong 
Published: February 2023

Abstract
Background
Acute liver failure (ALF) is one of the most common life-threatening diseases in adults without previous liver disease, and the construction of animal models using D- galactosamine(D-Gal) in combination with lipopolysaccharide(LPS) has been widely used to study the mechanisms of ALF and for drug development. Glycogen synthase kinase 3β (GSK3β) is a serine/threonine protein kinase that is widely distributed in the cells. Inhibition of its activity can inhibit cell death and promote autophagy through various pathways, thus providing a protective effect. In this study, we aimed to investigate the effect on ALF after inhibition of GSK3β and its potential mechanisms.
Methods
A mouse ALF model was constructed using LPS/D-Gal, while D-Gal/TNF-α stimulated L02 cells were used to construct an in vitro model. After TDZD-8 treatment TUNEL staining and flow techniques were used to detect the proportion of apoptosis in liver tissues and cells respectively, while western blotting and immunofluorescence assays were performed to detect the expression levels of apoptosis, pyroptosis and necroptosis-related proteins in tissues and cells. In addition, to explore the specific mechanism of hepatoprotective effect after GSK3β inhibition, western blotting was performed to detect the expression levels of TAK1, TRAF6 and HDAC3 after TRAF6 and HDAC3 inhibition alone. The co-localization of TRAF6 and HDAC3 in L02 cells was detected by immunofluorescence, while the interaction between TRAF6 and HDAC3 was detected by immunoprecipitation assay.
Results
In both in vivo and in vitro experiments, we observed the hepatoprotective effect of GSK3β inhibitor TDZD-8 in ALF model through in vivo and in vitro experiments and found that inhibition of GSK3β activity could significantly reduce the level of hepatocyte apoptosis, pyroptosis, necroptosis and improve liver dysfunction and tissue damage. Furthermore, we found that hepatocyte TAK1 and TRAF6 levels decreased and HDAC3 levels increased in ALF, whereas inhibition of GSK3β upregulated TAK1 and TRAF6 levels and decreased HDAC3 expression.
Conclusion
This study investigated the protective effect of GSK3β inhibitor TDZD-8 on ALF, and its action may involve the TRAF6/HDAC3/TAK1 pathway.
Spatial variations in PΜ2.5 physico-chemical characteristics and associated health risk in an urban coastal city
Authors: Tripathi Shruti , Chakraborty Abhishek , Mandal Debayan 
Published: February 2023

Abstract

This paper investigates the chemical composition of Particulate Matter, Organic Carbon (OC), and Elemental Carbon (EC) in residential and traffic sites in Mumbai. The average PM2.5 and PM10 concentrations at the traffic site (Sakinaka) were 240 µg/m3 and 424 µg/m3, respectively. The observed levels of OC were 35 µg/m3, 22 µg/m3, and 15.5µg/m3 at Sakinaka junction (high-density traffic), YP-Gate (low-density traffic), and Hostel Premise (Residential), respectively. The average OC/EC ratio value was high (4.5) at the residential site, indicating contributions from stationary combustion sources and secondary production of carbonaceous species to OC. The residential site has a higher percentage of low volatile OC fraction (57%) in total OC than the traffic sites. On the other hand, Sakinaka has a higher percentage of highly volatile OC fractions (36%) in total OC. The crustal-originated metals were dominating in all areas, but the concentration of metals from anthropogenic sources was highest at Sakinaka, i.e., As (381 ng/m3), Pb (352 ng/m3), Zn (679 ng/m3). The K/Al, Ca/Al, Mg/Al, and Fe/Al ratios were high in all the samples compared to the crustal ratio indicating biomass burning and traffic emission sources of these metals. PM originating from traffic was more enriched with heavy metals that are toxic to human health, increasing cancer risks (CR) through inhalation. The hazard quotient was above 1 at all the locations, and CR was above 1x10− 4, causing health risks. According to the dosimetry model, more PM was deposited in the lungs of traffic location occupants through inhalation, increasing the cancerous risk.