bluuebunny/crossref_metadata_2025_split huggingface dataset:
Low Attainment of Treatment Targets for Cardiovascular Risk Factors in Indonesian Adults With Established Coronary Artery Disease
Authors: Arsyad Dian Sidik , Qalby Nurul , Qanitha Andriany , Mappangara Idar , Milayanti Wilis , Wahiduddin  , Ansariadi  , Visseren Frank L. J. , Cramer Maarten J. , Doevendans Pieter A. , Hageman Steven H. J. 
Published: January 2025

ABSTRACTRationaleEstablished coronary artery disease (CAD) patients are at increased risk for recurrence of cardiovascular events and mortality due to non‐attainment of recommended risk factor control targets.ObjectiveWe aimed to evaluate the attainment of treatment targets for risk factor control among CAD patients as recommended in the Indonesian CVD prevention guidelines.MethodsPatients were consecutively recruited from the Makassar Cardiac Center at Wahidin Sudirohusodo Hospital, Indonesia. Targets for systolic blood pressure (SBP < 140 mmHg), low‐density lipoprotein‐cholesterol (LDL‐C < 1.8 mmol/L), body mass index (BMI: 20–25 kg/m2), non‐smoking status and antithrombotic use were assessed according to the national guideline. Attainment levels were evaluated in CAD population subgroups using logistic regression to identify patients who required more intensive monitoring.ResultsA total of 395 CAD patients (median age: 57 years (IQR: 49–65), 63.8% men) were recruited between February and June 2021. We observed that 1.8% of the CAD patients met all risk factor treatment targets. LDL‐C had the lowest attainment level (5.1%), followed by BMI (59.7%), SBP (62.8%), antithrombotic usage (76.7%) and non‐smoking status (94.4%). Apart from lower attainment of SBP control in the 50+ years age group (aOR: 0.57, 95% CI: 0.35–0.94) and women (aOR: 0.53, 95% CI: 0.34–0.83), the attainment levels of other risk factors were consistently low across age, sex, marital status and educational subgroups.ConclusionThese findings emphasize the urgent need for effective management and heightened awareness, particularly for controlling LDL‐C in the CAD population. Action to address this issue is crucial for mitigating the CVD burden, particularly in low‐ and middle‐income countries such as Indonesia.
Regulation of Dendrite and Dendritic Spine Formation by TCF20
Authors: Vinci Ersilia , Beretta Stefania , Colombo Veronica , Zippo Antonio , Catanese Alberto , Wiegreffe Christoph , Britsch Stefan , Boeckers Tobias , Verpelli Chiara , Sala Carlo 
Published: January 2025

ABSTRACTMutations in the Transcription Factor 20 (TCF20) have been identified in patients with autism spectrum disorders (ASDs), intellectual disabilities (IDs), and other neurological issues. Recently, a new syndrome called TCF20‐associated neurodevelopmental disorders (TAND) has been described, with specific clinical features. While TCF20's role in the neurogenesis of mouse embryos has been reported, little is known about its molecular function in neurons. In this study, we demonstrate that TCF20 is expressed in all analyzed brain regions in mice, and its expression increases during brain development but decreases in muscle tissue. Our findings suggest that TCF20 plays a central role in dendritic arborization and dendritic spine formation processes. RNA sequencing analysis revealed a downregulation of pre‐ and postsynaptic pathways in TCF20 knockdown neurons. We also found decreased levels of GABRA1, BDNF, PSD‐95, and c‐Fos in total homogenates and in synaptosomal preparations of knockdown TCF20 rat cortical cultures. Furthermore, synaptosomal preparations of knockdown TCF20 rat cortical cultures showed significant downregulation of GluN2B and GABRA5, while GluA2 was significantly upregulated. Overall, our data suggest that TCF20 plays an essential role in neuronal development and function by modulating the expression of proteins involved in dendrite and synapse formation and function.image
Transcranial Doppler Arterial Pressure Gradient Is Associated With Delayed Infarction After Subarachnoid Hemorrhage
Authors: Kim Yongwoo , Kim Alexander , Williams Josef D. , Withington Charles , Tefera Eshetu , Gizaw Samrawit , Felbaum Daniel R. , Mai Jeffrey C. , Liu Ai‐Hsi , Armonda Rocco A. , Chang Jason J. 
Published: January 2025

ABSTRACTBackground and PurposeWhile the pulsatility index (PI) measured by transcranial Doppler (TCD) has broader associations with outcomes in neurocritical care, its use in monitoring delayed cerebral infarction (DCI) in patients with aneurysmal subarachnoid hemorrhage (SAH) is not endorsed by current clinical guidelines. Recognizing that arterial pressure gradient (ΔP) can be estimated using PI, we investigated the potential significance of TCD‐estimated ΔP.MethodsIn this observational study of 186 SAH patients, we recorded the mean cerebral blood flow velocity (mCBFV) and PI values from the middle cerebral artery, along with corresponding blood pressures. Using a previously reported mathematical model, we estimated ΔP by dividing pulse pressure by PI. We investigated the association between ΔP and mCBFV values and two acute phase complications of SAH—DCI and angiographic vasospasm. Additionally, we explored the association between DCI, vasospasm, and 90‐day functional outcome.ResultsElevated ΔP was associated with DCI (odds ratio [OR] 1.021, 95% confidence interval [CI] 1.004–1.039, p = 0.014) but not vasospasm (OR 1.006, 95% CI 0.991–1.022, p = 0.402). Elevated mCBFV was associated with vasospasm (OR 1.037, 95% CI 1.017–1.057, p < 0.001) but not DCI (OR 0.998, 95% CI 0.979–1.018, p = 0.873). DCI (OR 29.380, 95% CI 2.930–294.615, p = 0.004), rather than vasospasm (OR 0.695, 95% CI 0.120–4.043, p = 0.686), was associated with functional outcome.ConclusionsIncreased ΔP, rather than elevated mCBFV, was associated with DCI. While elevated mCBFV was associated with vasospasm, it was not associated with DCI. Hence, TCD‐estimated ΔP may serve as a predictor for the DCI in SAH patients, a condition that impacts long‐term outcome.
Risk Factors in Addition to Short and Long‐Term Outcomes With Thin Catheter Surfactant Administration Failure in Preterm Infants: A Retrospective Analysis
Authors: Kutman Hayriye Gozde Kanmaz , Bilgin Betül Siyah , Buyuktiryaki Mehmet , Simsek Gulsum Kadioglu , Üstünyurt Zeynep , Canpolat Fuat Emre 
Published: January 2025

ABSTRACTObjectiveTo evaluate the incidence of thin catheter surfactant administration (TCA) failure and compare short and long‐term neonatal outcomes who failed TCA or did not.DesignSingle‐center retrospective cohort study. Infants between 25 and 30 weeks of gestational age with respiratory distress syndrome and receiving 200 mg/kg poractant alfa via thin catheter administration were included. TCA failure was defined as the need for early mechanical ventilation (< 72 h). Infants were divided into two groups those who failed TCA or those who did not.ResultsThe TCA failure rate was 24.6%. Initial oxygen requirement (0.39% vs. 0.36%) and the number of small for gestational age infants were significantly higher in the TCA failure group (15% vs. 7.9%). Infants who failed TCA had a higher pneumothorax (6.7% vs. 1.1%, p = 0.03), BPD (15% vs.5.5%, p = 0.02), late‐onset sepsis (36.7% vs. 18%, p = 0.04), retinopathy of prematurity rates (11.7% vs. 3.3%, p = 0.02) and an increased duration of respiratory support. However, Bayley Scales of Infant Development II scores were comparable between groups at 18 and 26 months of corrected age.ConclusionInfants who fail TCA are at increased risk for short‐term complications despite favourable long‐term neurodevelopmental outcomes. Identifying infants at risk of TCA failure may help early prevention of morbidities and individualise their management.
Comparative Analysis of the Benefits of Glutathione‐Rich Yeast Hydrolysate and Astaxanthin on Growth Performance, Antioxidant Capacity and Lipid Metabolism in Pacific White Shrimp (Litopenaeus vannamei)
Authors: Chen Jiandong , Zhang Ling , Yang Fan , Yi Jianhua , Yang Zhilong , Li Zhaowen , Cheng Tao , Hu Xiangna , Tan Beiping , Cao Aiqiao , Chi Shuyan 
Published: January 2025

ABSTRACTAntioxidants, both glutathione (GSH) and astaxanthin (AX), possess beneficial applications in animal growth and antioxidant properties. In this study, three experimental diets with isoproteic and isolipidic were formulated, the control diet (CON), the control diet added with 0.03% Carophyll Pink (contains 10% AX), the control diet added with 0.67% glutathione‐rich yeast hydrolysate (GYH, contains 5% GSH). The effects of AX and GYH on the growth performance, antioxidant capacity and lipid metabolism of Litopenaeus vannamei (0.36 ± 0.01 g) were compared. After an 8‐week feeding experiment, shrimp growth and intestinal morphology showed significantly better performance in the GYH group. Both dietary AX and GYH regulated the expression levels of genes related to lipid metabolism, increased antioxidant enzyme activities and total antioxidant capacity of shrimp and reduced haemolymph malondialdehyde and hepatopancreas reactive oxygen species production. Compared with the CON and GYH groups, dietary AX significantly increased the redness (a*) and yellowness (b*) values of cooked shrimp. Based on the evaluation of the above results, AX and GYH were comparable in terms of antioxidant performance, and AX was superior in improving the red body colour of shrimp, but GYH has more significant advantages in promoting shrimp growth and intestinal development. Therefore, supplementation with GYH or AX in diet presents specific benefits to the growth, body colour and antioxidant of L. vannamei.
Diagnostic Accuracy of Microbiome‐Derived Biomarkers in Periodontitis: Systematic Review and Meta‐Analysis
Authors: Dong Anbo , Proctor Gordon , Zaric Svetislav 
Published: January 2025

ABSTRACTAimTo evaluate the diagnostic accuracy of microbiome‐derived biomarkers for periodontitis in oral fluids (saliva and subgingival samples).MethodsThis systematic review followed PRISMA guidelines. Electronic searches were performed across multiple databases from December 2022 to November 2024. Subgroup analyses, divided into saliva and subgingival samples, were performed using the Random Effects Model (REM), while individual biomarker sensitivity and specificity were evaluated through the Bivariate Random‐Effects Model (BREM).ResultsTen studies were included, stratified by sample type. In the saliva group, Porphyromonas gingivalis, Tannerella forsythia and Prevotella intermedia demonstrated the highest diagnostic accuracy, with sensitivities reaching 89.2%, 89.2% and 86.5%, and specificities of 94.6%, 86.5% and 83.8%, respectively, achieving AUC values above 0.80. Porphyromonas gingivalis was further analysed using BREM, with the Summary Receiver Operating Characteristic (SROC) curve indicating a combined sensitivity and specificity of 84.2% and 85.4%, with an AUC of 0.864. In the subgingival group, biomarkers such as endotoxin activity and combined bacterial biomarkers (5 bacterial species) displayed the highest diagnostic performance, with sensitivities of 90.6% and 85.1% and specificities of 87.9% and 100%, respectively, and AUC values of 0.93 and 0.88.ConclusionMicrobiome‐derived biomarkers show good clinical utility for improving diagnoses of periodontitis, offering high specificity and sensitivity. Future research should focus on standardising methodologies, increasing sample sizes, and including diverse populations to validate these findings, thereby improving diagnostic precision and facilitating the screening methods for the onset of periodontitis and dysbiotic activity.
Use of a periodontal resective procedure (proximal wedge technique) for treating gingival and periodontal pockets of mandibular canine teeth in dogs
Authors: Mathis J. R. , Scott K. I. , Lobprise H. B. 
Published: January 2025

ObjectivesThis study aims to evaluate the ability of a periodontal resective procedure (the Proximal Wedge Technique) to improve the long‐term health of the mandibular canines by reducing probing depths immediately post‐surgery via targeted tissue recontouring and precise debridement. The technique aims to enhance periodontal health and stabilize the supporting structures of strategically important adjacent canine teeth, including alveolar bone, rather than relying solely on mandibular incisor extractions with simple closure.Materials and MethodsA retrospective records review compiled data from patients with suprabony pockets of 6 mm or greater at the mesial aspect of the mandibular canines treated with the proximal wedge technique (PWT). The PWT utilizes non‐displaced gingival flaps combining concepts from the modified Widman flap and distal wedge procedures with selective osteoplasty.ResultsTwenty‐one mandibular canine sites from 12 patients of various breeds with pocket depths ranging from 6 to 10 mm were treated with the PWT resulting in post‐operative mean pocket depths of 1.7 ± 0.1 mm. Approximately 1 year later, the improved depths were unchanged in six of the patients comprising 12 of the 21 sites.Clinical SignificanceBy employing this method, veterinarians can enhance periodontal health and stabilize the supporting structures of strategically important adjacent canine teeth, including alveolar bone, rather than relying solely on mandibular incisor extractions with simple closure. The Proximal Wedge Technique resulted in reliable periodontal success and improved patient outcomes for one year and potentially longer, allowing retention of the mandibular canines and maintaining the integrity of the rostral mandible.
Effectiveness of vapocoolant spray compared to eutectic lidocaine/prilocaine cream to enhance tolerance during intravenous catheterisation: a randomised controlled trial
Authors: Trinder R. , Pak J. , Humm K. , Cole L. 
Published: January 2025

ObjectivesTo determine if tolerance of intravenous catheterisation differs following the application of vapocoolant spray compared to lidocaine/prilocaine cream in dogs and cats.Materials and MethodsA randomised controlled trial of client‐owned dogs and cats requiring intravenous catheterisation was performed. They were randomly allocated to either have lidocaine/prilocaine cream applied to their skin 1 hour prior to intravenous catheterisation or a swab saturated with vapocoolant spray applied immediately prior to intravenous catheterisation. The procedure was video‐recorded and a single blinded observer reviewed the recordings and assigned reaction scores (0 to 3) at 4 time points (initial restraint, limb handling, swab application and skin puncture).ResultsBetween October 2020 and March 2022, a total of 101 animals (83 dogs and 18 cats) were enrolled, with 56 patients randomised to receive vapocoolant spray and 45 to receive lidocaine/prilocaine cream. There was no significant difference in the age, sex status, number of cross and pure breeds, and mentation detected between the groups. There was no significant difference in reaction scores between the treatments when comparing all patients at any time point except for a significantly increased swab application reaction score in patients receiving vapocoolant spray. Vapocoolant spray was significantly less effective in reducing adverse reaction to skin puncture than lidocaine/prilocaine cream in the small number of cats evaluated.Clinical SignificanceWhen considering all patients together, no single method of anaesthesia appeared superior for improving tolerance of intravenous catheter placement. However, vapocoolant spray may be less effective than lidocaine/prilocaine cream in reducing adverse response to skin puncture during catheterisation in cats.
Perioperative analgesic effects of a modified supratemporal retrobulbar block in dogs undergoing corneal and endocular surgery
Authors: Lardone E. , Crasta M. , Ostan P. C. , Gherlinzoni P. , Landi A. , Franci P. 
Published: January 2025

ObjectivesTo evaluate the perioperative efficacy of a modified supratemporal retrobulbar block in dogs undergoing ocular surgery.Materials and MethodsIn this prospective randomized clinical trial, dogs were premedicated with dexmedetomidine (1 mcg/kg im) and methadone (0.1 mg/kg im), induced with propofol to effect and maintained with isoflurane (FE'Iso 1.1%). In the retrobulbar group a mixture of lidocaine 2% (5.5 mL) and ropivacaine 0.75% (2 mL) was administered at 0.1 mL/kg, via a modified supratemporal technique using a Tuohy needle. No block was performed in the controls. When heart rate or mean arterial pressure increased above 30% of the pre‐incisional values, fentanyl (1 mcg/kg iv) was administered. Propofol (1 mg/kg iv) was injected when anaesthesia was deemed too light. After a total of three administrations regardless of the type of drugs (fentanyl/propofol), a constant rate infusion of fentanyl (5 mcg/kg/h iv) was started. Quality of recovery (blindly assessed using a descriptive score scale), postoperative eye rubbing and complications were studied.ResultsEighteen dogs were included. The retrobulbar group (nine) dogs had significantly less risk of receiving fentanyl than controls (nine) (Relative risk:  0.142, 95% CI: 0.021 to 0.936) and a recovery score > 2 (RR: 0.058, 95% CI: 0.003 to 0.887). The median amount of fentanyl (mcg/kg) was statistically lower in the retrobulbar group than in the controls: 0 mcg/kg (range, 0 to 1) versus 2 mcg/kg (range, 0 to 8.49), respectively. Only controls showed eye rubbing.Clinical SignificanceThe modified supratemporal retrobulbar block reduced the intraoperative rescue analgesia and improved the quality of recovery.
Population Pharmacokinetics of Enrofloxacin in Ctenopharyngodon idella Based on the Sparse Sampling Method and a Nonlinear Mixed Effect Model Following Intravenous and Oral Administration
Authors: Xu Ning , Zhang Huan , Zhou Shun , Liu Yongtao , Yang Qiuhong , Dong Jing , Ding Yongzhen , Ai Xiaohui 
Published: January 2025

ABSTRACTThe objective of this study was to implement population pharmacokinetic (PPK) of enrofloxacin (EF) in grass carp (Ctenopharyngodon idella) after a single oral administration and a single intravenous administration based on a nonlinear mixed effect model. The plasma samples collected by the sparse sampling method were detected by high‐performance liquid chromatography with a fluorescent detector. The initial pharmacokinetic (PK) parameters were evaluated by reference search and the calculation of a naïve pooled method. After oral administration, the concentration–time profile was best described by a one‐compartment open model. The absorption rate constant (Ka), apparent distribution volume (V), and systemic clearance (CL) were estimated to be 3.11/h, 4.36 L/kg, and 0.079 L/h/kg, respectively. After intravenous administration, the concentration–time curve was best simulated by a two‐compartment open model. The apparent distribution volume of the central compartment (V1), apparent distribution volume of the peripheral compartment (V2), CL, and clearance from the central compartment to the peripheral compartment (CL2) were estimated to be 0.42, 2.05 L/kg, 0.067, and 2.94 L/h/kg, respectively. Finally, the bioavailability was calculated to be 84.81%. The parameter of AUC/minimum inhibitory concentration value was estimated to be more than 506.32 for Aeromonas hydrophila, Aeromonas sobria, and Flavobacterium columnare indicating that EF at 20 mg/kg has high effectiveness for these pathogens. This study supported a concise method for conducting PK study in aquatic animals that facilitated the development of PK methodology in aquaculture.
Estimating Fisheries Reference Points for Five Data‐Limited Fish Stocks in Kaptai Reservoir, Bangladesh
Authors: Khatun Mst Halima , Zahangir Md. Mahiuddin , Akhter Bably , Naziat Azmaien , Liu Qun 
Published: January 2025

ABSTRACTThe Kaptai reservoir is one of the important fisheries hubs in Bangladesh for its significant role in the national economy and socio‐economic sector. However, easy accessibility to the reservoir along with other human‐driven disturbances are pushing the stocks at risk. The sustainable fishery management depends on estimating a stock's total allowable catch (TAC), therefore, a recently developed Monte Carlo method (CMSY) was used for five data‐limited stocks (catfish, Sperata aor; snakehead, Channa marulius; carp, Labeo rohita; cichlids, Oreochromis mossambicus and featherback, Notopterus notopterus) to estimate their fishery reference points using their catch, resilience and exploitation record at the first and last catch of the year. A Bayesian state‐space implementation of the Schaefer surplus production model (BSM) was also used to compare and justify the output of the CMSY. CMSY estimated the maximum sustainable yield (MSY) to be 378, 110, 74.5, 432 and 99 mt for S. aor, C. marulius, L. rohita, O. mossambicus and N. notopterus, respectively. The result suggests that C. marulius, L. rohita and O. mossambicus fisheries were rebuilding naturally and at underfishing and underfished state according to the values of F/FMSY and B/BMSY as per BSM (value < 1). Value of BMSY greater than present biomass estimated by BSM for L. rohita and O. mossambicus predicts that the stock's overexploitation state seeking serious attention. Although the S. aor fishery seemed to be sustainable and had the potential to produce more, F/FMSY and B/BMSY values close to 1 recommend strict management actions for further sustainability. However, F/FMSY 1.31 for N. notopterus reveals that the stock depleted remarkably making this fishery unsustainable. The study reveals that Kaptai reservoir fisheries require intensive care to maintain sustainable resources. Strict implication of allowable catch limit, mesh size regulation, maintaining water quality, and protection of breeding and nursery ground is highly recommended.
A habitat suitability analysis for three Culicoides species implicated in bluetongue virus transmission in the Southeastern United States
Authors: Kessinger Peter , James Angela M. , Patyk Kelly A. , Vigil Stacey L. , Ruder Mark G. , Magzamen Sheryl 
Published: January 2025

AbstractCulicoides biting midges adversely impact animal health through transmission of multiple orbiviruses, such as bluetongue virus (BTV). This study used light trapping data collected in the Southeastern United States for three Culicoides midge species that are confirmed or suspected BTV vectors: Culicoides insignis, Culicoides stellifer and Culicoides venustus. Midge presence datasets were combined with meteorological data and ecological data to model habitat suitability for each species. Logistic regression and machine learning models were used to generate individual species distribution models (SDMs). Results for each SDM method were combined in an ensemble model to create a distribution model for each midge species. Based on overlay analyses of livestock populations and midge suitable habitat, there is extensive overlap of cattle and goat populations and suitable habitat for C. insignis in Florida. Suitable habitat for C. stellifer intersects with cattle and goat populations in various counties in Alabama, Arkansas, the Carolinas, Florida, Georgia, Louisiana and Tennessee; and suitable habitat for C. venustus intersects with cattle and goat populations in the same states as C. stellifer, except for Florida. It is critical for orbivirus and midge surveillance to continue in the Southeastern United States as the habitat of all three midge species intersect with livestock populations.