bluuebunny/crossref_metadata_2025_split huggingface dataset:
Functional defects in hiPSCs-derived cardiomyocytes from patients with a PLEKHM2-mutation associated with dilated cardiomyopathy and left ventricular non-compaction
Authors: Korover Nataly , Etzion Sharon , Cherniak Alexander , Rabinski Tatiana , Levitas Aviva , Etzion Yoram , Ofir Rivka , Parvari Ruti , Cohen Smadar 
Published: June 2023

AbstractDilated cardiomyopathy (DCM) is a primary myocardial disease, leading to heart failure and excessive risk of sudden cardiac death with rather poorly understood pathophysiology. In 2015, Parvari's group identified a recessive mutation in the autophagy regulator, PLEKHM2 gene, in a family with severe recessive DCM and left ventricular non-compaction (LVNC). Fibroblasts isolated from these patients exhibited abnormal subcellular distribution of endosomes, Golgi apparatus, lysosomes and had impaired autophagy flux. To better understand the effect of mutated PLEKHM2 on cardiac tissue, we generated and characterized induced pluripotent stem cells-derived cardiomyocytes (iPSC-CMs) from two patients and a healthy control from the same family. The patient iPSC-CMs showed low expression levels of genes encoding for contractile functional proteins (α and β-myosin heavy chains and 2v and 2a-myosin light chains), structural proteins integral to heart contraction (Troponin C, T and I) and proteins participating in Ca2+pumping action (SERCA2 and Calsequestrin 2) compared to their levels in control iPSC-derived CMs. Furthermore, the sarcomeres of the patient iPSC-CMs were less oriented and aligned compared to control cells and generated slowly beating foci with lower intracellular calcium amplitude and abnormal calcium transient kinetics, measured by IonOptix system and MuscleMotion software. Autophagy in patient’s iPSC-CMs was impaired as determined from a decrease in the accumulation of autophagosomes in response to chloroquine and rapamycin treatment, compared to control iPSC-CMs. Impairment in autophagy together with the deficiency in the expression of NKX2.5, MHC, MLC, Troponins and CASQ2 genes, which are related to contraction-relaxation coupling and intracellular Ca2+signaling, may contribute to the defective function of the patient CMs and possibly affect cell maturation and cardiac failure with time.
COVID-19 and FDI nexus in Pakistan: fresh evidence from QARDL and time-varying casualty techniques
Authors: Chishti Muhammad Zubair 
Published: June 2023

Abstract
While the COVID-19 pandemic’s detrimental repercussions on global economic growth are not exactly measured, there is widespread agreement among the policy-makers that it can deteriorate the global economy drastically. To this end, several studies have endeavored to analyze the harmful effects of COVID-19 on economic activities; however, the scholars mainly focus on the developed nations and less attention is paid to the developing economies. Hence, the considerable effects of COVID-19 necessitate to more research in this area specifically for developing economies. To fill this literature gap, the recent article tends to divulge the impacts of COVID-19 on one of the vital macroeconomic variables, i.e., foreign direct investment inflows (FDI) with the consort of energy prices (EPI) and real exchange rates (EXR) for Pakistan. To this end, we deploy the two proxies (viz., new deaths and new cases) for COVID-19 to find the reliable and more directional results. For analysis purpose, we use the several advanced econometric techniques. The results of QARDL suggest that COVID-19 significantly reduces the FDI inflows in Pakistan due to rise in COVID-19, while employing the both proxies. However, based on the results, we suggest that the variable of new deaths is more reliable proxy to capture the effects of COVID-19. Similarly, we infer that EPI and EXR also lead to decrease the FDI inflows. Besides, the quantile Granger causality and TVGC tests also support our results by confirming the casual nexus from COVID-19 and EPI to FDI.
Corporate governance, external financing, and earnings management: new evidence from an emerging market
Authors: Hong Nguyen Thi Hoa , Anh Nguyen Thai , Hoang Nguyen Tran Viet , Minh Do Nhat 
Published: June 2023

Abstract
This study investigates the relationship between corporate governance, external financing, and earnings management in an emerging market. Using a sample of Vietnamese listed companies in the period of 2010–2020, the results indicate that corporate governance, which is measured by a principal component analysis (PCA) methodology, is a useful mechanism to control earnings management. However, when firms engage in external financing activities, corporate governance is not significantly associated with both accrual-based and real earnings management. In addition, the study also examines the role of corporate governance in moderating the effects of earnings management on firm value, and how it is encouraged by external financing needs. The study shows that while good corporate governance lessens the influence of earnings management on firm value, external financing needs only prompts earnings manipulation and have no effect on firm value, directly or indirectly. Therefore, the findings could provide implications for managers and regulators to enhance governance practice to alleviate firm devaluation caused by earnings management practice.
‘Medical clearance’ and referral to liaison psychiatry: a national service evaluation
Authors: Gillett George , Westwood Sophie , Thomson Alex B. , Lee William 
Published: June 2023

Aims and methodThe prevalence of delaying psychiatric care until the patient has received ‘medical clearance’, and the definitions and understanding of ‘medical clearance’ terminology by relevant clinicians, are largely unknown. In a service evaluation of adult liaison psychiatry services across England, we explore the prevalence, definitions and understanding of ‘medical clearance’ terminology in three parallel studies: (a) an analysis of trust policies, (b) a survey of liaison psychiatry services and (c) a survey of referring junior doctors. Content and thematic analyses were performed.Results‘Medical clearance’ terminology was used in the majority of trust policies, reported as a referral criterion by many liaison psychiatry services and had been encountered by most referring doctors. ‘Medical clearance’ was identified as a common barrier to liaison psychiatry referral. Terms were inconsistently used and poorly defined.Clinical implicationsMany liaison psychiatry services seem not to comply with guidance promoting parallel assessment. This may affect parity of physical and mental healthcare provision.
Psychiatric in-patients who are parents: what interventions are tailored to their needs and how do they experience care? A systematic review and data synthesis
Authors: Dunn Abigail , Christiansen Hanna , Elsby-Pearson Chloe , Kramer Jaqueline , Swinburn Eliza , Platt Belinda , Cartwright-Hatton Sam 
Published: June 2023

Background
Little is known about the experiences of parents who are in receipt of in-patient psychiatric care or about what interventions are employed to support them in their parenting role.

Aims
The objective of the current study is to review two complementary areas of research: (a) research examining interventions developed to support the parent–child relationship within these settings; and (b) research focused on the experience of parents in in-patient settings.

Method
For studies reporting on parents’ experience, qualitative accounts of past or present psychiatric in-patients (child aged 1–18 years) were included. For intervention studies, the intervention had to focus on supporting the parenting role and/or the parent–child dyad of parents (child aged 1–18 years) in current receipt of in-patient care. Four bibliographic databases (PubMed, SCOPOS, Web of Science and PsychINFO) were searched for relevant published and unpublished literature from 1 January 1980 to 26 July 2022. Intervention studies were appraised using the Mixed Methods Appraisal Tool. Qualitative papers were assessed using the Critical Appraisal Skills Programme tool. Data were extracted using tools designed for the study. Qualitative data were synthesised using thematic analysis. The protocol was registered with the International Prospective Register of Systematic Reviews (reference CRD42022309065).

Results
Twenty-four papers (eight intervention studies and 16 studies examining parent experience) were included in the review. In-patient parents commonly reported hospital admission as having a negative impact on their parenting. Very few robust reports of interventions designed to support parents in receipt of psychiatric in-patient care were found.

Conclusions
Despite the identified need for support by parents who are receiving in-patient care, there is currently no intervention of this nature running in the UK health service.
Zaisheng yuan and the Writing of Women's Culture
Authors: Epstein Maram 
Published: June 2023

Abstract
This article focuses on Zaisheng yuan as an intertextual work of creative fiction that draws from male-authored xiaoshuo fiction as well as earlier literary tanci novels. This case study discusses Zaisheng yuan as a key text in an affective archive of narrative works written by women that provides insights into how elite women mentally negotiated the social and ideological expectations that informed their lives. In addition to looking at how women authors rewrote the conventions associated with scholar-beauty romances, the chastity cult, and the gendered symbols associated with proper order, this article discusses tanci novels as a unique outlet for women's explorations of autonomous will (zhi 志) and imaginings of emotional justice.
Retinoic acid is dispensable for meiotic initiation but required for spermiogenesis in the mammalian testis
Authors: Kirsanov Oleksandr , Johnson Taylor A. , Niedenberger Bryan A. , Malachowski Taylor N. , Hale Benjamin J. , Chen Qing , Lackford Brad , Wang Jiajia , Singh Anukriti , Schindler Karen , Hermann Brian P. , Hu Guang , Geyer Christopher B. 
Published: June 2023

ABSTRACT
Retinoic acid (RA) is the proposed mammalian ‘meiosis inducing substance’. However, evidence for this role comes from studies in the fetal ovary, where germ cell differentiation and meiotic initiation are temporally inseparable. In the postnatal testis, these events are separated by more than 1 week. Exploiting this difference, we discovered that, although RA is required for spermatogonial differentiation, it is dispensable for the subsequent initiation, progression and completion of meiosis. Indeed, in the absence of RA, the meiotic transcriptome program in both differentiating spermatogonia and spermatocytes entering meiosis was largely unaffected. Instead, transcripts encoding factors required during spermiogenesis were aberrant during preleptonema, and the subsequent spermatid morphogenesis program was disrupted such that no sperm were produced. Taken together, these data reveal a RA-independent model for male meiotic initiation.
Muscle-on-a-chip devices: a new era for in vitro modelling of muscular dystrophies
Authors: Fernández-Costa Juan M. , Tejedera-Vilafranca Ainoa , Fernández-Garibay Xiomara , Ramón-Azcón Javier 
Published: June 2023

ABSTRACT
Muscular dystrophies are a heterogeneous group of highly debilitating diseases that result in muscle atrophy and weakness. The lack of suitable cellular and animal models that reproduce specific aspects of their pathophysiology is one of the reasons why there are no curative treatments for these disorders. This highlights a considerable gap between current laboratory models and clinical practice. We strongly believe that organs-on-chip could help to fill this gap. Organs-on-chip, and in particular muscles-on-chip, are microfluidic devices that integrate functional skeletal muscle tissues. Biosensors in these systems allow monitoring of muscle homeostasis or drug responses in situ. This Perspective outlines the potential of organs-on-chip as advanced models for muscular dystrophies, as well as the current challenges and future opportunities for this technology.
Translocation of cytosolic human Cdc73 to stress granules plays a role in arsenic stress-induced stabilization of p53 mRNA
Authors: Lee Hojin , Kim Tae-Hyeon , Yoo Joo-Yeon 
Published: June 2023

ABSTRACT
Cells trigger the assembly of stress granules (SGs) under various stress conditions. Among the many proteins recruited to SGs are RNA-binding proteins and transcription regulators. Here, we report the translocation of human (h)Cdc73, a component of the PAF1 transcription complex, to cytosolic SGs in response to arsenic stress. The hCdc73 protein possesses a long intrinsically disordered region (IDR) from amino acids 256–416, the presence of which is required for the translocation of hCdc73 to cytosolic SGs. The purified hCdc73 IDR formed droplets in vitro, and the light-activated assembly of hCdc73-IDR–mCherry–CRY2 was verified. For translocation of hCdc73 to SGs, physical interactions with SG carrier proteins, such as FMR1, are also needed. Previously, we reported that the cytosolic hCdc73–eEF1Bγ complex controls the stability of p53 mRNA. Under arsenic stress, selective sequestration of cytosolic hCdc73, but not eEF1Bγ (EEF1G) or p53 (TP53) mRNA, was detected. As a result, a transient increase in p53 mRNA at the post-transcriptional level was observed. In conclusion, we propose that the availability of mRNAs for stress-responsive genes can be controlled by restraining their negative regulators within SGs.
Population-specific responses in eastern oysters exposed to low salinity in the northern Gulf of Mexico
Authors: Sirovy Kyle A. , Casas Sandra M. , La Peyre Jerome F. , Kelly Morgan W. 
Published: June 2023

ABSTRACTEastern oysters, Crassostrea virginica, are facing rapid environmental changes in the northern Gulf of Mexico and can respond to these changes via plasticity or evolution. Plastic responses can immediately buffer against environmental changes, although this buffering may impact the organism's ability to evolve in subsequent generations. While plasticity and evolution are not mutually exclusive, the relative contribution and interaction between them remains unclear. In this study, we investigated the roles of plastic and evolved responses of C. virginica acclimated to low salinity using a common garden experiment with four populations exposed to two salinities. We used three transcriptomic analyses (edgeR, PERMANOVA and WGCNA) combined with physiology data to identify the effect of genotype (population), environment (salinity) and the genotype–environment interaction on both whole-organism and molecular phenotypes. We demonstrate that variation in gene expression is mainly driven by population, with relatively small changes in response to salinity. In contrast, the morphology and physiology data reveal that salinity has a larger influence on oyster performance than the population of origin. All analyses lacked signatures of the genotype×environment interaction and, in contrast to previous studies, we found no evidence for population-specific responses to low salinity. However, individuals from the highest salinity estuary displayed highly divergent gene expression from that of other populations, which could potentially drive population-specific responses to other stressors. Our findings suggest that C. virginica largely rely on plasticity in physiology to buffer the effects of low salinity, but that these changes in physiology do not rely on large persistent changes in gene expression.
13-Year Associations of Occupational and Leisure-Time Physical Activity with Cardiorespiratory Fitness in CARDIA
Authors: Quinn Tyler D. , Lane Abbi , Gabriel Kelley Pettee , Sternfeld Barbara , Jacobs David R. , Smith Peter , Gibbs Bethany Barone 
Published: June 2023

ABSTRACT

Purpose
Differential effects on fitness are hypothesized to contribute to the opposing health effects of leisure-time physical activity (LTPA) and occupational physical activity (OPA). As such, this study examined cross-sectional and longitudinal associations of fitness with LTPA and OPA.

Methods
This study examined fitness associations with LTPA and OPA across 13 years in the Coronary Artery Risk Development in Young Adults (CARDIA) Study (years 7 [baseline], 10, 15, and 20 [follow-up] exams). Fitness was measured at baseline and follow-up via symptom-limited maximal graded exercise test (GXT) duration (seconds) while LTPA and OPA were self-reported during each exam. Baseline and follow-up cross-sectional associations of LTPA (low, medium, high) and OPA (0, 1-6, and ≥ 6 months with OPA) with fitness were examined using linear regression. Longitudinal linear regression examined associations between 13-year LTPA (low, medium, or high) and OPA (no, decreasing, or increasing) trajectories with fitness at follow-up, adjusted for baseline values. All models adjusted for center, sex, race, age, education, smoking history, alcohol intake, resting blood pressure, diabetes status, and body mass index. Stratified analyses examined associations by sex (female/male), race (Black/White), and LTPA groups.

Results
Compared to low, medium and high LTPA were positively associated with fitness in all analyses (p < 0.001). Reporting 1-6 or ≥ 6 months with OPA was negatively associated with fitness in cross-sectional follow-up models (β = -15.6 and -15.4 respectively, p ≤ 0.01). Longitudinally, those with increasing OPA had lower follow-up fitness compared to no OPA (β = -16.41, p < 0.01). Negative associations of OPA with fitness were not meaningfully different across sex and race groups. Significant LTPA by OPA interactions were observed (p < 001).

Conclusions
Physical activity research and public health promotion should consider domain-specific associations on cardiovascular health.
Transient Pseudohypoaldosteronism: A Rare Cause of Severe Hyponatremia in a Baby
Authors: D’Amico F. , Pomi A. Li , Busceti D. , Zirilli G. , Salzano G. , Cucinotta U. , Cassone C.M. , Vazzana G.F. , Valenzise M. 
Published: June 2023

Hyponatremia and dehydration in children represent a medical emergency due to a variety of underlying illness. Other than an evidence of gastroenteritis with diarrhea and vomiting (which is the major cause of hypoosmolar hyponatremia in pediatric age), other causes should be considered, especially if there is evidence of hyperkalemia and high sodium fraction excretion (FENa), like iatrogenic causes (diuretic excess), transient or genetic abnormalities of the renal mineralocorticoid pathway, syndrome of inappropriate anti-diuretic hormone secretion (SIADH), acute renal failure, congenital adrenal hyperplasia (CAH).
Here we present a case of transient pseudohypoaldosteronism in a 2 months old baby secondary to urinary tract infection, who presented with a history of poor sucking, fever and dehydration.
Policy coordination and integration in local government: perspectives on barriers
Authors: De Wulf Nicolai , Voets Joris , Molenveld Astrid 
Published: June 2023

The topic of policy integration has become an important component of several distinct lines of policy research. However, the concept of policy integration is notoriously difficult to specify and observe. This article provides valuable insights that explore the subjectivity of responses by political and administrative officials at the local level charged with policy integration. In response to its central question: ‘How do local politicians and civil servants perceive politico-administrative barriers facing policy coordination and integration?’ the study reveals political and managerial perceptions of key politicians and civil servants in local governments about the barriers to achieving policy integration.
By contrast with other studies involving politico-administrative relations, it draws on a broader institutional context, inclusive of the complex and dynamic environment and local level environment. Its findings show that significant barriers to coordination and integration continue to limit the policy coordination and integration needed to address complex societal challenges.
Using Q methodology, three perspectives emerge which provide crucial insights into the main barriers preventing integration. These findings adeptly depict the complex relationship between vertical, hierarchical relationships between politicians and bureaucrats, and the requirement for a more horizontal, consensual relationship in order to meet crosscutting societal challenges.
In this way, the article offers a significant contribution to the scholarship on policy integration.
Association between migration and severe maternal outcomes in high-income countries: Systematic review and meta-analysis
Authors: Eslier Maxime , Azria Elie , Chatzistergiou Konstantinos , Stewart Zelda , Dechartres Agnès , Deneux-Tharaux Catherine 
Published: June 2023

Background
Literature focusing on migration and maternal health inequalities is inconclusive, possibly because of the heterogeneous definitions and settings studied. We aimed to synthesize the literature comparing the risks of severe maternal outcomes in high-income countries between migrant and native-born women, overall and by host country and region of birth.

Methods and findings
Systematic literature review and meta-analysis using the Medline/PubMed, Embase, and Cochrane Library databases for the period from January 1, 1990 to April 18, 2023. We included observational studies comparing the risk of maternal mortality or all-cause or cause-specific severe maternal morbidity in high-income countries between migrant women, defined by birth outside the host country, and native-born women; used the Newcastle–Ottawa scale tool to assess risk of bias; and performed random-effects meta-analyses. Subgroup analyses were planned by host country and region of birth.
The initial 2,290 unique references produced 35 studies published as 39 reports covering Europe, Australia, the United States of America, and Canada. In Europe, migrant women had a higher risk of maternal mortality than native-born women (pooled risk ratio [RR], 1.34; 95% confidence interval [CI], 1.14, 1.58; p < 0.001), but not in the USA or Australia. Some subgroups of migrant women, including those born in sub-Saharan Africa (pooled RR, 2.91; 95% CI, 2.03, 4.15; p < 0.001), Latin America and the Caribbean (pooled RR, 2.77; 95% CI, 1.43, 5.35; p = 0.002), and Asia (pooled RR, 1.57, 95% CI, 1.09, 2.26; p = 0.01) were at higher risk of maternal mortality than native-born women, but not those born in Europe or in the Middle East and North Africa. Although they were studied less often and with heterogeneous definitions of outcomes, patterns for all-cause severe maternal morbidity and maternal intensive care unit admission were similar. We were unable to take into account other social factors that might interact with migrant status to determine maternal health because many of these data were unavailable.

Conclusions
In this systematic review of the existing literature applying a single definition of “migrant” women, we found that the differential risk of severe maternal outcomes in migrant versus native-born women in high-income countries varied by host country and region of origin. These data highlight the need to further explore the mechanisms underlying these inequities.

Trial Registration
PROSPERO CRD42021224193.