bluuebunny/crossref_metadata_2025_split huggingface dataset:
Intraspecific divergence of sexual size dimorphism and reproductive strategies in a polytypic poison frog
Authors: Justicia Lia Schlippe , Mayer Martin , Lorioux-Chevalier Ugo , Dittrich Carolin , Rojas Bibiana , Chouteau Mathieu 
Published: July 2023

Abstract
Intraspecific variation in body size, both among populations and between sexes, is an important factor influencing life-history strategies. This variation might be the response to different environmental conditions, as well as natural and sexual selection, and can result in differences in behavior and reproductive strategies among populations. Here, we use the dyeing poison frog (Dendrobates tinctorius) as a model to investigate how interpopulation variation in body size and sexual size dimorphism affects reproductive strategies. As body size increased, sexual size dimorphism also increased, i.e., females were larger than males, and more so in populations with overall larger frogs. This indicates that there is a stronger selection for body size in females than in males, likely as a response to divergent reproductive investment between the sexes. Females from larger-bodied populations produced larger clutches, but the overall number of froglets produced per clutch did not differ among populations. We discuss potential causes and mechanisms that might be responsible for the observed divergence in body size, sexual size dimorphism, and reproductive strategies among populations that likely represent local adaptations, which might affect future population divergence and ultimately speciation. Our findings demonstrate the importance of cross-population studies, cautioning against drawing general conclusions about a species' ecology without accounting for intraspecific variation.
WITHDRAWN: Preliminary study on seasonal diversity of root endophytic fungi and bacteria associated with sainfoin (Onobrychis viciifolia) in South Africa
Authors: Slabbert Marcell S. , Rashidifard Milad , Ashrafi Samad , Claassens Sarina 
Published: July 2023

Abstract

Sainfoin (Onobrychis viciifolia), is a beneficial forage legume, which is adapted to a wide range of climatic conditions. It has the potential to be an exceptionally useful forage crop due to its high digestibility and palatability, anthelmintic properties, endophytes, and abundance of nectar and pollen for honey production. We report here the first attempts to study the diversity of endophytic fungi and bacteria associated with sainfoin in South African agricultural lands during dry and wet season.. This was achieved by isolating, cultivating, purifying, and molecular characterising the fungi and bacteria from the roots of sainfoin obtained from an established agricultural field during both the wet and dry seasons. During the dry season, 15 fungal isolates and 10 bacterial isolates were obtained from sainfoin roots, which consisted of 9 fungal genera and 4 bacterial genera, respectively. While, during the wet season 28 fungal isolates and 24 bacterial isolates, representing 12 fungal genera and 12 bacterial genera were successfully isolated. A comprehensive literature-based discussion on the potential plant growth-promoting effects of the obtained fungal and bacterial isolates was also conducted to preliminary identify those with potential for agricultural application. The study showed that sainfoin roots could interact with a diverse group of endophytes, which is greatly influenced by seasonality, with a higher diversity and isolation rate during the wet season in South Africa.
Host-derived growth factors drive ERK phosphorylation and MCL1 expression to promote osteosarcoma cell survival during metastatic lung colonization
Authors: McAloney Camille A , Makkawi Rawan , Budhathoki Yogesh , Cannon Matthew V , Franz Emily M , Gross Amy C , Cam Maren , Vetter Tatyana A , Duhen Rebekka , Davies Alexander E , Roberts Ryan D 
Published: July 2023

AbstractPurpose For patients with osteosarcoma, disease-related mortality most often results from lung metastasis—a phenomenon shared with many solid tumors. While established metastatic lesions behave aggressively, very few of the tumor cells that reach the lung will survive. By identifying mechanisms that facilitate survival of disseminated tumor cells, we can develop therapeutic strategies that prevent and treat metastasis. Methods We analyzed single cell RNA-sequencing (scRNAseq) data from murine metastasis-bearing lungs to interrogate changes in both host and tumor cells during colonization. We used these data to elucidate pathways that become activated in cells that survive dissemination and identify candidate host-derived signals that drive activation. We validated these findings through live cell reporter systems, immunocytochemistry, and fluorescent immunohistochemistry. We then validated the functional relevance of key candidates using pharmacologic inhibition in models of metastatic osteosarcoma. Results Expression patterns suggest that the MAPK pathway is significantly elevated in early and (to a lesser degree) established metastases. MAPK activity correlates with expression of anti-apoptotic genes, especially MCL1. Niche cells produce growth factors that increase ERK phosphorylation and MCL1 expression in tumor cells. Both early and established metastases are vulnerable to MCL1 inhibition, but not MEK inhibitionin vivo. Combining MCL1 inhibition with chemotherapy both prevented colonization and eliminated established metastases in murine models of osteosarcoma. Conclusion Niche-derived growth factors drive MAPK activity and MCL1 expression in osteosarcoma, promoting metastatic colonization. Although later metastases produce less MCL1, they remain dependent on it. MCL1 is a promising target for clinical trials in both human and canine patients.
Efficient turn-on Zr based metal organic framework fluorescent sensor for ultrafast detection of danofloxacin in milk samples
Authors: Verma Rajpal , Dhingra Gaurav , Singh Gurdeep , Singh Jaswinder , Dureja Nidhi , Malik Ashok Kumar 
Published: July 2023

Abstract
Metal organic framework, UiO-67 was synthesized by coordinating Zr(IV) with 4,4'-biphenyldicarboxylic acid (BPDC) ligand. Morphology and crystallinity of MOF was confirmed with FE-SEM and PXRD procedure. Danofloxacin (DANO), a veterinary antibiotic, was detected in milk by employing UiO-67 as “turn-on” fluorescent sensor. Original photoluminescent (PL) efficiency of UiO-67 sensor was enhanced on its electronic interaction with DANO molecule. Significant PL efficiency enhancement, lower detection limit 0.49 ng/mL (1.37 nM), swift detection (< 1 min), and excellent linear correlation (R2 = 0.9988) indicated extraordinary sensitivity of developed MOF sensor for DANO. Selectivity and performance of sensor was unaltered in presence of interfering species and detection results were obtained under permissible variation limits. Method applied successfully for ultra-trace detection of DANO residues in milk samples.
WITHDRAWN: Performance Analysis of Hybrid Plasmonic Terahertz Waveguide fed Patch Antenna
Authors: Pallavi Mahankali , Mondal Shyamal , Thipparaju Rama Rao 
Published: July 2023

Abstract
To achieve high data rates and higher bandwidth, Terahertz (THz) radiation is a promising candidate for next generation wireless communications. This research study demonstrates that performance analysis of THz hybrid plasmonic waveguide fed patch antenna constructed around 2.5 to 3.5 THz. A hybrid plasmonic THz waveguide was used as a feed line for designed THz patch antenna to increase the antenna efficiency. The proposed plasmonic waveguide fed antenna consists of Gallium Arsenide (GaAs), a high index material enclosed by low index material of Aluminium Arsenide (AlAs) and silver (Ag) to confine the light inside the GaAs region. A graphene film is placed between high density polyethylene (HDPE) in order to increase the light confinement. The variation of width and height of the GaAs region, the parameters effective refractive index, mode area, dispersion, confinement loss, birefringence, beat length, waveguide fed antenna gain, efficiency and bandwidth have been studied using mode analysis by finite element method. Utilizing finite element approach, the simulation results of proposed hybrid plasmonic THz waveguide fed patch antenna shows effective refractive index of 3.79, birefringence of 0.2, beat length of 123 mm, mode area of 1.88 mm2, mode field diameter of 15.8 mm, normal dispersion of 0.10 ps/THz/cm, low confinement loss of 1.79 x 10− 9 mm− 1, waveguide fed antenna gain of 5.11dBi, bandwidth of 112.9 GHz, efficiency of 76% in the range of 2.5 to 3.5 THz. The proposed THz waveguide fed antenna can be feasible for various photonic integrated circuits for future THz communication applications.
Limitations of U25 CKiD and CKD-EPI eGFR formulae in patients 2-20 years of age with measured GFR&gt;60 mL/min/1.73 m2 – A cross-sectional study
Authors: Filler Guido , Ahmad Fateh , Bhayana Vipin , de Ferris Maria Esther Díaz González , Sharma Ajay Parkash 
Published: July 2023

Abstract
Background: When applying Pierce U25 formula for estimating glomerular filtration rate (eGFR), we observed a higher proportion of eGFR<90 mL/min/1.73 m2 (chronic kidney disease=CKD stage 2). We compared agreement and accuracy of the Pierce U25 (ages 2-25), Pottel (ages 2-100), and CKD-EPI (ages 18-100) formulae to GFR measurements.
Methods: Post-hoc analysis of the three eGFRs compared to 367 99m technetium-diethylene-triamine penta-acetic acid (99Tc DTPA) GFR measurements (240 patients) using 3 sampling points and Brockner/Mørtensen correction (body surface area calculation based on ideal weight) on simultaneous serum creatinine and cystatin C measurements.
Results: Overall, the U25 formula performed well with a Spearman r of 0.8102 (95% confidence interval 0.7706 to 0.8435, p<0.0001) while diagnostic accuracy was low in patients with normal mGFR. The U25 formula reclassified 29.5% of patients with normal mGFR as CKD stage 2; whereas the average of the modified Schwartz formula based on serum creatinine and the Filler formula based on cystatin C, only over diagnosed CKD stage 2 in 8.5%, 24.5% within 10% and 62.7% within 30%. We therefore combined both. The average Schwartz/Filler eGFR had 36.5% of results within 10%, 84.7% within 30%, and normal mGFR accuracy was 26.8%, 63.9% for 10% and 30%, respectively, outperforming the CKD-EPI and Pottel formulae.
Interpretation: The Pierce U25 formula results correlated well with mGFR<75 mL/min/1.73m2. Over the entire GFR range, accuracy was better for patients with a higher mGFR, when averaging the combined Schwartz/Filler formulae. More work is needed to prospectively confirm our findings in other centers.
Newly discovered Pituitary Adenoma in the Emergency Center during the COVID-19 Pandemic in Serbia: Treatment and Early Postoperative Outcome
Authors: Radanović Dražen , Kaluđerović Dea , Pajić Srbislav , Slijepčević Nikola , Knežević Saša , Jakovljević Sofija , Elboursh Dušan , Bogosavljević Đurđina , Mirčić Uroš , Čukić Bojan 
Published: July 2023

Abstract
Introduction:Pituitary tumors represent 10-15% of all intracranial tumors. Clinical manifestations depend on the size of the tumor such as microadenoma, macroadenoma or giant adenoma, and type of the tumor (secreting or non-secreting). Surgical treatment of pituitary adenoma can be managed via transcranial or transsphenoidal approach. Depending on the approach, there is a possibillity of postoperative complications such as meningitis, pneumocephalus, liquorrhea, transient diabetes insipidus and ect.
Aim:The aim of this study was to establish the frequency of newly discovered pituitary tumors in the Emergency Center, University Clinical Center Of Serbia, during the COVID-19 pandemic in Serbia, and early postoperative complications in patients treated with an endoscopic and microscope transsphenoidal approach.
Material and methods: During the time period during the pandemic, the study contains 119 patients, from January 1, 2020 to March 1, 2023, of which 64 are male (53.8%) and 55 are female (46.2%), age range is 14 to 85 years with a mean of 52.10. As for statistical analysis, assessment of frequency rate and relative numbers were used as methods of descriptive statistics.
Results:Macroadenoma was present in 95 patients (79.83%), microadenoma in 22 patients (18.49%), and giant adenoma in 2 patients (1.69%). Transient DI developed postoperatively in 11 patients (9.24%). The average duration of hospitalization after surgery was 8 days.
Conclusion: The duration of hospitalization depends on numerous factors where the COVID-19 pandemic can serve as an example for future similar crisis situations so that better organization and preoperative preparation of patients can be implemented.
Abnormal Global Longitudinal Strain During Anthracycline Treatment Predicts Future Cardiotoxicity in Children
Authors: Gunsaulus Megan , Alsaied Tarek , Tersak Jean M. , Friehling Erika , Rose-Felker Kirsten 
Published: July 2023

Abstract
Background
Global longitudinal strain (GLS) is a sensitive predictor of cardiotoxicity in adults with cancer. However, the significance of abnormal GLS during childhood cancer treatment is less well-understood.
Objectives
The objective was to evaluate the use of GLS for predicting later cardiac dysfunction in pediatric cancer survivors exposed to high dose anthracyclines.
Methods
This was a retrospective study of pediatric patients exposed to a doxorubicin isotoxic equivalent dose of ≥ 225mg/m2. Transthoracic echocardiograms (TTE) were obtained prior to chemotherapy (T1), during anthracycline therapy (T2), and following completion of therapy (T3). Cardiotoxicity was defined as meeting at least one of the following criteria after anthracycline therapy: a decrease in left ventricle ejection fraction (LVEF) by 10% from baseline to a value < 55%, fractional shortening < 28%, or a decrease in GLS by ≥ 15% from baseline.
Results
Nineteen of 57 (33%) patients met criteria for cardiotoxicity at T3. Cardiotoxicity was associated with a lower LVEF at T2 (p = 0.0003) and a decrease in GLS by ≥ 15% at T2 compared to baseline (p = < 0.0001). ROC analysis revealed that the best predictor of cardiotoxicity at T3 was the percent change in GLS at T2 compared to baseline (AUC 0.87). A subgroup analysis revealed that a decrease in GLS by ≥ 15% from baseline at 0–6 months from completion of anthracycline therapy was associated with cardiotoxicity > 1-year post-treatment (p = 0.017).
Conclusions
A decline in GLS during chemotherapy was the best predictor of cardiotoxicity post-treatment. GLS serves as an important marker of cardiac function in pediatric patients undergoing treatment with anthracyclines.
Assessing the Influence of Commercial Activity Characteristics Spatially on Human Behavior Post-COVID-19 Pandemic
Authors: Mabrouk Mahmoud , Han Haoying , Abdrabo Karim I. , Shen Guoqiang 
Published: July 2023

Abstract
Recently, various infectious diseases have emerged, some of which have evolved into world-threatening pandemic outbreaks. Since December 2019, the disastrous COVID-19 has spread rapidly worldwide, claiming many human lives, and dragging the global economy to a standstill with a dramatic recession. Among the ramifications are the transformations of commercial activity because of the prejudicial impact of the lockdown: a shift of sales channels towards online alternatives, accompanied by procedures for temporary closures or a move into the periphery regions with low operational expenses. Our research has significantly identified the influence of the characteristics of commercial uses on human behavioral changes in two Egyptian cities, namely the 6th of October and Sheikh Zayed, as a case study, in achieving self-sufficiency for residents during devastating outbreaks. Depending on geospatial software, descriptive geostatistical analysis, and semi-structured interviews, the study found that in both cities, new small commercial centers and complexes with more open spaces became more attractive than large malls by 74%. Also, new commercial forms are positively correlated with open spaces and negatively correlated with population density (estimated at.992 and − .366, respectively). This result may guide policymakers to transfer from huge malls to smarter, greener, dispersed malls during city planning and from monocentric urban structures to polycentric and linear commercial centers, shaping pandemic-resilient cities.
A systematic computational analysis of the endosomal recycling pathway in glioblastoma
Authors: Joyce Luke J. , Lindsay Andrew J. 
Published: July 2023

Abstract

Purpose
Glioblastoma (GBM) is the most common and most aggressive brain cancer in adults. Approximately 250,000 new cases are diagnosed worldwide per year, and its incidence is rising due to our aging population. The standard treatment is brutal and has not changed in 20 years, and more than 85% of patients will die within two years of their diagnosis. There is thus an urgent need to identify new drug targets and develop novel therapeutic strategies that will increase survival and improve quality of life.
Methods
Using publicly available genomics, transcriptomics and proteomics datasets, we show that key regulators of endosomal recycling are dysregulated in GBM and are linked to survival outcomes.
Results
We report here that an intracellular membrane trafficking pathway called the endosomal recycling pathway represents a novel target that could be exploited for the development of small molecule inhibitors to treat people with GBM. We report an 8-gene endosomal recycling prognostic signature that can distinguish low-risk from high-risk GBM and potentially identify tumours that may benefit from endosomal recycling inhibitors.
Conclusion
This study presents the first systematic analysis of the endosomal recycling pathway in glioblastoma and proposes that it is a promising target for the development of novel therapies and therapeutic strategies that could improve the outcomes for patients with glioblastoma.
On behavior of transversely isotropic incompressible hyperelastic materials under multiaxial stretching
Authors: Methia Mounir , Bechir Hocine , Yaya Kamel 
Published: July 2023

Abstract
Nowadays, mechanical behavior of soft materials are characterized by using standard tests: uniaxial traction, simple shear (or combination) and equibiaxial tension. However, the multiaxial testing data are necessary in order to characterize the anisotropy, which is posing formidable technical challenges. We propose a new method to investigate multiaxial behavior of fiber-reinforced hyperelastic solids. The test protocol consists to deform a thin rectangular sample by coupling both the pure shear and simple shear loadings. The specimen is mounted and oriented by an angle in the plane of a special device, which is attached to a universal testing machine. So, the direction of stretching is dictated by in such way that, the stress-strain relations depend on and magnitude of the displacement prescribed on the sample edge, i.e. . The shape factor of the sample is assumed to dictate the direction of transverse isotropy in the reference configuration. Thereby, the direction of stretching could be taken neither parallel nor perpendicular to the stretching-direction. But also, it could be chosen parallel to the direction of transverse isotropy. Based on, we have revisited the standard results, established new ones, and investigated the anisotropy. We demonstrated that, the Cauchy stress fields are depending on the multiaxiality in the core region of the sample. So, the experimental data arising from this test could be used for both parameter calibration and/or validation of transversely isotropic incompressible hyper elastic models.
An amino acid mixture, enriched with Krebs cycle intermediates, enhances extracellular matrix gene expression in cultured human fibroblasts
Authors: Ragni Maurizio , Canciani Luca , Spataro Letizia , Ruocco Chiara , Valerio Alessandra , Nisoli Enzo 
Published: July 2023

Abstract
In the human body, the skin is one of the organs most affected by the aging process. Nutritional approaches aimed to counteract the age-induced decline of extracellular matrix (ECM) deposition could be a valuable tool to decrease the degenerative processes underlying skin aging. Here we investigated the ability of a six-amino acid plus hyaluronic acid (6AAH) formulation enriched with tricarboxylic acid (TCA) intermediates to stimulate ECM gene expression. To this aim, human BJ fibroblasts were supplemented with 6AAH alone or plus succinate or malate alone or succinate plus malate (6AAHSM), and mRNA levels of several ECM markers were evaluated. 6AAHSM increased the expression of all the ECM markers significantly above 6AAH alone or plus only succinate or malate. Furthermore, in an in vitro oxidative stress model, 6AAHSM blunted the hydrogen peroxide-induced decline in ECM gene expression. Our data suggest that dietary supplementation with 6AAH enriched with TCAs could efficiently be employed as a non-pharmacological approach for counteracting skin aging.
The Influence of Varying Sound Pitch on the Brain’s Electrical Activities of Young Nigerian Adults
Authors: Odigie Osagie Mike. , Anyaehie Ugochukwu Bond , Nwachukwu Daniel Chukwu , Igweh John Chukwuka , Naiho Alexander Obidike 
Published: July 2023

Abstract
There is a considerable interest in understanding the Brain’s electrical activity; at least to better the understanding of arousal and mental wakefulness in different conscious states, including in conditions like seizures. Electroencephalography is a technique for assessing Brain’s electrical function, and has been adapted for use in humans. Desynchronized waveforms occur frequently in humans during the sleep cycle, leading to a substantial alteration in serum levels of neuro-endocrine hormones for proper daily routines. In this study, we assessed the influence of pitch; a key ingredient in sounds on the electrical patterns of the brain of young Nigerian adults. Being a cross-sectional observation study, 400 healthy young Nigerians (16–35 years) from the students’ community of the Delta State University, Abraka, Delta State of Nigeria were exposed to varying pre-recorded sound pitches (30 Hz, 40 Hz, 50 Hz and 60 Hz) for 5 minutes each; haven determined their brainwave patterns [Alpha (α), Beta (β), delta (δ), Theta (θ) and Gamma (γ) rhythms] at rest (baseline) in a sound-proof studio, using the electroencephalogram (EEG). Statistical measures of association (correlation) was conducted, mean differences (ANOVA) computed with the graph pad prison (version 8.1), while adjudging p-value < 0.05 as statistically significant. Results suggest that lower frequency sounds (30Hz, 40 Hz) elicited α, or δ rhythms in relaxed cortical state, although insignificantly. ANOVA returned no significant change (p > 0.05) in brain activities compared to baseline. A weak negative correlation was observed (Pearson’s coefficient) between pitch and participants’ γ, θ and δ waveforms, even though this was insignificant (p > 0.05). Whereas, β and α activities returned strong and weak positive correlation respectively with increasing pitch administration. Thus, varying sound pitch increased brain’s electrical activities in exposed youths, although insignificantly.