bluuebunny/crossref_metadata_2025_split huggingface dataset:
Strategi Guru Dalam Menanamkan Nilai Pendidikan Karakter Peduli Lingkungan Di TK Negeri Pembina Penukal Pali
Authors: Vitri Wina Adha , Handayani Tutut , Cindryah Elsa 
Published: June 2023

Penelitian ini berjudul Strategi Guru Dalam Menanamkan Nilai Pendidikan Karakter Peduli Lingkungan di TK Negeri Pembina Penukal. Dengan rumusan masalah: bagaimana strategi guru dalam menanamkan pendidikan karakter peduli lingkungan pada peserta didik di TK Negeri Pembina Penukal dan apa faktor pendukung dan penghambat guru dalam menanamkan pendidikan karakter peduli lingkungan di TK Negeri Pembina Penukal. Adapun penelitian ini bertujuan untuk mendeskripsikan Strategi Guru Dalam Menanamkan Nilai Pendidikan karakter peduli lingkungan di TK Negeri Pembina Penukal. Dalam penelitian ini, peneliti menggunakan penelitian deskriptif kualitatif. Informan penelitian yang dilakukan dalam penelitian ini yaitu Kepala Sekolah, peneliti dan Guru sebagai informan pendukung. Adapun teknik pengumpulan data dalam penelitian ini melalui observasi, wawancara, dan dokumentasi. Sedangkan teknik yang digunakan dalam analisis data adalah reduksi data, penyajian data, dan penarikan kesimpulan, sehingga data dapat diketahui dengan baik.Hasil penelitian ini menunjukkan bahwa Strategi guru yang digunakan di Tk Negeri Pembina Penukal dalam menanamkan nilai pendidikan karakter peduli lingkungan yaitu menggunakan metode pembiasaan, metode keteladanan, contohnya seperti melaksanakan kegiatan tanam-menanam bunga, bergotong royong membersikan lingkungan sekolah, dan membuang sampah pada tempatnya.Adapun faktor pendukung dalam menanamkan nilai pendidikan karakter peduli lingkungan di TK Negeri Pembina Penukal adalah guru sebagai teladan yang baik bagi siswa seperti memberi contoh membuang sampah sampah pada tempatnya, menjaga ucapan-ucapan dan membudayakan senyum, sapa.
Peran Guru Dalam Mengembangkan Sosial Emosional Anak Di Tk Darul Muttaqin Desa Bulu Sari Kecamatan Bumi Ratu Nuban Lampung Tengah
Authors: Kholifah Siti Nur , Rizqiyani Revina 
Published: June 2023

Social-emotional development is critical in the process of child development which is formed through the developments obtained in a learning process both from the family environment, friendship environment, and school environment. In developing children's social-emotional, teachers have a very important role in developing children's social-emotional at school. This study aims to find out how the role of the teacher in developing children's social-emotional in Darul Muttaqin Kindergarten, Bumi Ratu Nuban District, Central Lampung. This type of research uses descriptive qualitative field research, which was conducted at Darul Muttaqin Kindergarten, Bumi Ratu Nuban District, Central Lampung. The data sources used are primary data sources and secondary data sources. The data collection method used is interview, observation and documentation. The data analysis techniques used are data reduction, data presentation, and data verification. To test the validity of the data using source triangulation and technical triangulation. Based on the results of the research and analysis of the data obtained, it can be concluded that in developing the social emotionality of children in Kindergarten Darul Muttaqin, Bumi Ratu Nuban District, the teacher acts as an educator, supervisor, manager, innovator, communicator and motivator in order to develop children's social emotional development so that they can develop optimally.

Keywords: teacher's role, social emotional development, early childhood
Empirical Estimation of the Haezendonck-Goovaerts Risk
Authors: Miao Kathleen E. 
Published: June 2023

<p> In this thesis, we describe and study the empirical Haezendonck-Goovaerts (HG) risk measure. First, we introduce the HG risk measure, and provide the mathematical construction of the HG measure. We then show that the HG risk measure is coherent, and describe estimation methods. We perform extensive numerical experiments, and conclude that the optimization methodology of estimating risk with the HG has significant flaws. We find that the empirical method of estimation is a significant improvement over estimation via optimization with respect to both speed and accuracy, and that the empirical method has the behaviours of asymptotic normality and weak convergence. Finally, we perform a case study with a Canadian portfolio, and compare the results to the well-known and well-used risk measure expected shortfall. </p>
Kinerja Keuangan Sebagai Pemoderasi Good Corporate Governance, Corporate Social Responsibility dengan Nilai Perusahaan
Authors: Mau Maria Selviana Tresna , Kadarusman Kadarusman 
Published: June 2023

Tujuan dari penelitian ini menguji dan menganalisis pengaruh GCG dan pengungkapan CSR terhadap nilai perusahaan serta kinerja keuangan sebagai variabel pemoderasi. Dalam penelitian ini variabel GCG diproksikan dengan kepemilikian manajerial. Variabel kinerja keuangan diproksikan dengan ROA. Objek penelitian yang digunakan adalah perusahaan pertambangan yang terdaftar di Bursa Efek Indonesia periode 2017-2019. Penelitian ini menggunakan teknik purposive sampling dalam menentukan sampel penelitian. Diperoleh 22 perusahaan sebagai sampel penelitian sesuai dengan kriteria sampel yang telah ditentukan. Metode analisis yang digunakan adalah Moderated Regression Analysis (MRA). Hasil penelitian ini menunjukkan bahwa GCG tidak berpengaruh signifikan terhadap nilai perusahaan, pengungkapan CSR berpengaruh signifikan terhadap nilai perusahaan. kinerja keuangan tidak mampu memoderasi hubungan GCG terhadap nilai perusahaan. Kinerja keuangan mampu memoderasi hubungan pengungkapan CSR terhadap nilai perusahaan.
High-flow oxygen therapy for ventilator weaning patients with tracheostomy cannula
Authors: Koryakin A. G. , Vlasenko A. V. , Evdokimov E. A. , Makovei V. I. , Erofeev V. V. , Osipov S. A. , Rodionov E. P. , Slastnikov E. D. , Klyuev I. S. 
Published: June 2023

Introduction. To date, information of the use of high-flow oxygen therapy (HFOT) in patients with tracheostomy cannulas is limited. The use of HFOT in this group of patients at the ventilator weaning may improve outcomes.Objective. Improving results of treatment of patients with tracheostomy cannulas with hypoxemic ARF of various origin through the use of HFOT at the ventilator weaning after prolonged mechanical ventilation (MV).Materials and methods. The prospective open cohort study included 50 patients aged 45 (from 37 to 57) years, the duration of invasive mechanical ventilation was 8.68 ± 3.83 days. Patients were randomized into two groups: in group A, weaning from the respirator was performed through a combination of traditional low-flow oxygen therapy (LFO) and assisted modes of MV; in group B – through a combination of HFOT and assisted modes of MV. The groups compared gas exchange rates, the duration of weaning from the respirator, the incidence of complications (pulmonary and extrapulmonary), the duration of treatment, mortality in the ICU and in the hospital. In all patients, the most significant predictors of in-hospital mortality were analyzed.Results. The use of HFOT in patients in group B revealed significant benefits in terms of the studied parameters in the early and late stages of the study. Mortality in the groups did not differ. The most significant risk factors for in-hospital mortality were cannulation after transfer from the OR (OR = 9,667; 95% CI: 2.414–38,713; p = 0,001).Conclusions. The use of HFOT in patients with tracheostomy cannulas is more clinically effective than COT. Risk factors assessment in every patient will allow optimal use HFOT.
Efficiency of fascia iliaca block in total hip arthroplasty
Authors: Krylov S. V. , Pasechnik I. N. , Guzhev S. S. , Orletskiy A. K. , Shumskiy A. A. , Dgebuadze G. , Gerasenkova A. D. 
Published: June 2023

Introduction. The present study examined the effect of iliac fascia blockade in patients after primary total hip arthroplasty (TEH).Purpose. Evaluation of the efficacy and safety of using the iliac fascia blockade in the postoperative period after TEH.Materials and methods. The study included 60 patients who underwent primary TEH. Patients were randomly divided into two groups: group 1 (n = 30) – patients who underwent a multimodal scheme of postoperative analgesia in the postoperative period; group 2 (n = 30) – patients who, in addition to multimodal analgesia, underwent blockade of the iliac fascia of the thigh under ultrasound navigation after surgery. In the postoperative period, the level of pain syndrome at rest and during movement was recorded using a visual analog scale of the pain level (VAS 1 and VAS 2) after 6–12–24–48 hours. We also recorded the frequency and quantity of prescription of narcotic analgesics and noted the frequency of side effects from their use.Results. The level of pain syndrome at rest and during movement in the first 24 hours was lower in patients of the 2nd group. After 48 hours, there were no significant differences between the groups in the level of pain. Patients in group 1 were prescribed opioid analgesics more often than patients in group 2. The number of side effects from the use of narcotic analgesics was greater in patients of group 1.Conclusions. The use of the iliac fascia block is an effective method of perioperative analgesia after hip arthroplasty. This method allows achieving a high level of pain relief and reduces the need for prescription of opioid analgesics.
Finite element evaluation of an American football helmet featuring liquid shock absorbers for protecting against concussive and subconcussive head impacts
Authors: Cecchi Nicholas J. , Vahid Alizadeh Hossein , Liu Yuzhe , Camarillo David B. 
Published: June 2023

Introduction: Concern has grown over the potential long-term effects of repeated head impacts and concussions in American football. Recent advances in impact engineering have yielded the development of soft, collapsible, liquid shock absorbers, which have demonstrated the ability to dramatically attenuate impact forces relative to existing helmet shock absorbers.Methods: To further explore how liquid shock absorbers can improve the efficacy of an American football helmet, we developed and optimized a finite element (FE) helmet model including 21 liquid shock absorbers spread out throughout the helmet. Using FE models of an anthropomorphic test headform and linear impactor, a previously published impact test protocol representative of concussive National Football League impacts (six impact locations, three velocities) was performed on the liquid FE helmet model and four existing FE helmet models. We also evaluated the helmets at three lower impact velocities representative of subconcussive football impacts. Head kinematics were recorded for each impact and used to compute the Head Acceleration Response Metric (HARM), a metric factoring in both linear and angular head kinematics and used to evaluate helmet performance. The head kinematics were also input to a FE model of the head and brain to calculate the resulting brain strain from each impact.Results: The liquid helmet model yielded the lowest value of HARM at 33 of the 36 impact conditions, offering an average 33.0% (range: −37.5% to 56.0%) and 32.0% (range: −2.2% to 50.5%) reduction over the existing helmet models at each impact condition in the subconcussive and concussive tests, respectively. The liquid helmet had a Helmet Performance Score (calculated using a summation of HARM values weighted based on injury incidence data) of 0.71, compared to scores ranging from 1.07 – 1.21 from the other four FE helmet models. Resulting brain strains were also lower in the liquid helmet.Discussion: The results of this study demonstrate the promising ability of liquid shock absorbers to improve helmet safety performance and encourage the development of physical prototypes of helmets featuring this technology. The implications of the observed reductions on brain injury risk are discussed.
Construction of an alpaca immune antibody library for the selection of nanobodies against Drosophila melanogaster proteins
Authors: Qiu Jianxiang , Li Jie , Zhang Zhen , Dong Shirui , Ling Xiaomei , Fang Zhixin , Ling Quanshou , Huang Zhixin 
Published: June 2023

Introduction:Drosophila melanogaster is a model organism for studying developmental biology and human neural disorders. Nanobodies are the variable domains of the heavy chains of camelid heavy-chain antibodies (VHHs) with high affinity to their antigens and have applications in basic research, similar to traditional antibodies. In addition, nanobodies acting as functionalized antibodies or protein binders have become an additional valuable approach in Drosophila. This study aimed to develop a VHH library against Drosophila proteins and confirm its availability by retrieving some Drosophila protein-specific nanobodies from the library.Methods: An alpaca was first immunized with Drosophila embryo lysate and then its lymphocytes were isolated. Total RNA was extracted and cDNA was synthesized. The vhh sequences were amplified by two round PCR, which were then ligated to a phage display vector pADL-10b. The ligation products were transduced into SS320 competent cells to generate a VHH library. From this library, nanobodies against CG7544, Myc, and CyclinE was enriched and screened by phage display technology and ELISA. DNA sequences of identified nanobodies were cloned into pADL-10b-Flag-His for expression and purification in Escherichia coli SS320. Binding ability of purified nanobodies with corresponding antigens were determined by ELISA and surface plasmon resonance in vitro.Results: In this study, an immune VHH library against Drosophila embryo proteins was generated with a capacity of 3 × 107. From this library, eight nanobodies against three Drosophila proteins, Myc, CyclinE, and CG7544, were identified and the DNA sequences of these nanobodies were obtained. These nanobodies were successfully expressed and purified from Escherichia coli SS320, and were demonstrated to bind corresponding antigens with high affinity in vitro. Moreover, the equilibrium constant between the highest enriched nanobodies and corresponding antigens were calculated.Conclusion: In summary, we report the availability of an immune VHH library and a highly efficient panning strategy for nanobodies against proteins in Drosophila.
Documenting the immune response in patients with COVID-19-induced acute respiratory distress syndrome
Authors: Lu Junyu , Zeng Xiaona , Lu Weisheng , Feng Jihua , Yang Yegui , Wei Yongxian , Chen Yin , Zhang Jianfeng , Pinhu Liao 
Published: June 2023

Introduction: Coronavirus disease 2019 (COVID-19) can lead to acute respiratory distress syndrome (ARDS) and life-threatening multi-organ failure with increased levels of inflammatory mediators and viral load; however, little is known about its pathophysiology.Methods: To better understand the cellular status of COVID-19-induced ARDS, we performed single-cell RNA sequencing on peripheral blood samples from patients with COVID-19-induced ARDS. Single-cell RNA sequencing combined with bioinformatics analysis was used to study dynamic changes in cell composition and transcriptional profiles.Results: The single-cell RNA sequencing data revealed significant phenotypic differences between patients with COVID-19-induced ARDS and controls, mainly in monocytes, and CD8+ T and B cells. B-cell and monocyte abundances were significant in COVID-19-induced ARDS patients compared to controls, while CD8+ T cells were depleted. These data suggest that there is an imbalance between lymphocytes and monocytes in the blood of COVID-19-induced ARDS patients. In addition, cytokine interactions between T cells, monocytes and B cells are enhanced as evidenced by the intercellular communication analysis. In particular, T cell subsets target receptors on other cells via CCL5 and may play an important role in patients with COVID-19-induced ARDS.Conclusion: Our analysis suggested that a dysregulated adaptive immune response exists in patients with COVID-19-induced ARDS. Overall, we provided a cellular picture of the peripheral immune response in patients with COVID-19-induced ARDS.
Endothelial progenitor cells derived from embryonic stem cells prevent alveolar simplification in a murine model of bronchopulmonary dysplasia
Authors: Kolesnichenko Olena A. , Flood Hannah M. , Zhang Yufang , Ustiyan Vladimir , Cuervo Jimenez Hayde K. , Kalin Tanya V. , Kalinichenko Vladimir V. 
Published: June 2023

Introduction: Vascular remodeling and compromised alveolar development are hallmarks of chronic pulmonary diseases such as bronchopulmonary dysplasia (BPD). Despite advances in neonatal healthcare the number of BPD cases worldwide continues to increase. One approach to overcoming the premature arrest in lung development seen in BPD is to stimulate neonatal angiogenesis via delivery and engraftment of endothelial progenitor cells (EPCs). One such population is resident to the pulmonary microvasculature and expresses both FOXF1 and c-KIT. Previous studies have shown that c-KIT+FOXF1+ EPCs are highly sensitive to elevated levels of oxygen (hyperoxia) and are decreased in premature infants with BPD and hyperoxia-induced BPD mouse models. We hypothesize that restoring EPCs through transplantation of c-KIT+FOXF1+ EPCs derived in vitro from pluripotent embryonic stem cells (ESCs), will stimulate neonatal angiogenesis and alveolarization in mice with hyperoxia-induced lung injury.Methods: Utilizing a novel ESC line with a FOXF1:GFP reporter, we generated ESC-derived c-KIT+FOXF1+ EPCs in vitro. Using a second ESC line which contains FOXF1:GFP and tdTomato transgenes, we differentiated ESCs towards c-KIT+FOXF1+ EPCs and tracked them in vivo after injection into the neonatal circulation of hyperoxia-injured mice. After a recovery period in room air conditions, we analyzed c-KIT+FOXF1+ EPC engraftment and quantified the number of resident and circulating endothelial cells, the size of alveolar spaces, and the capillary density after EPC transplantations.Results and conclusion: Herein, we demonstrate that addition of BMP9 to the directed endothelial differentiation protocol results in very efficient generation of c-KIT+FOXF1+ EPCs from pluripotent ESCs. ESC-derived c-KIT+FOXF1+ EPCs effectively engraft into the pulmonary microvasculature of hyperoxia-injured mice, promote vascular remodeling in alveoli, increase the number of resident and circulating endothelial cells, and improve alveolarization. Altogether, these results provide a proof-of-principle that cell therapy with ESC-derived c-KIT+FOXF1+ EPCs can prevent alveolar simplification in a hyperoxia-induced BPD mouse model.
Synthesis of acrylic resin and methacrylic resin microspheres by suspension polymerization
Authors: Yu Han , Liu Junjie , Zhao Lin , Liu Yonglin , Gu Lulu , Feng Lianxiang , Weng Yonggen , Duan Qingxu , Duan Baorong , Qu Jiale 
Published: June 2023

The process of suspension polymerization was utilized to create acrylate resin microspheres with mesh numbers of 140–200 μm and particle sizes of 100 μm for implementation in mesh coating technology. The copolymer of methyl methacrylate (MMA) and methyl acrylate (MA) served as the primary polymer, with dibenzoyl peroxide (DBPO) functioning as the initiator, and a mixture of calcium carbonate and deionized water served as the dispersion medium. The surface morphology of the synthesized microspheres was analyzed through Fourier-transform infrared (FTIR) spectroscopy and scanning electron microscopy (SEM) to confirm successful synthesis. The optimal reaction conditions for the synthesis of these microspheres were determined to be a dispersant dosage of 30 g of calcium carbonate with a monomer ratio of 4:1, a reaction time of 1 h, an initiator dosage of 1.2 g of BPO, and a reaction temperature of approximately 75–80 C, resulting in microspheres with a regular spherical shape and smooth surface.
Clustering single-cell multimodal omics data with jrSiCKLSNMF
Authors: Ellis Dorothy , Roy Arkaprava , Datta Susmita 
Published: June 2023

Introduction: The development of multimodal single-cell omics methods has enabled the collection of data across different omics modalities from the same set of single cells. Each omics modality provides unique information about cell type and function, so the ability to integrate data from different modalities can provide deeper insights into cellular functions. Often, single-cell omics data can prove challenging to model because of high dimensionality, sparsity, and technical noise.Methods: We propose a novel multimodal data analysis method called joint graph-regularized Single-Cell Kullback-Leibler Sparse Non-negative Matrix Factorization (jrSiCKLSNMF, pronounced “junior sickles NMF”) that extracts latent factors shared across omics modalities within the same set of single cells.Results: We compare our clustering algorithm to several existing methods on four sets of data simulated from third party software. We also apply our algorithm to a real set of cell line data.Discussion: We show overwhelmingly better clustering performance than several existing methods on the simulated data. On a real multimodal omics dataset, we also find our method to produce scientifically accurate clustering results.
Liposomes loaded with vitamin D3 induce regulatory circuits in human dendritic cells
Authors: Nagy Noémi Anna , Lozano Vigario Fernando , Sparrius Rinske , van Capel Toni M. M. , van Ree Ronald , Tas Sander W. , de Vries I. Jolanda M. , Geijtenbeek Teunis B. H. , Slütter Bram , de Jong Esther C. ,   for the DC4Balance consortium
Published: June 2023

IntroductionNanomedicine provides a promising platform for manipulating dendritic cells (DCs) and the ensuing adaptive immune response. For the induction of regulatory responses, DCs can be targeted in vivo with nanoparticles incorporating tolerogenic adjuvants and auto-antigens or allergens.MethodsHere, we investigated the tolerogenic effect of different liposome formulations loaded with vitamin D3 (VD3). We extensively phenotyped monocyte-derived DCs (moDCs) and skin DCs and assessed DC-induced regulatory CD4+ T cells in coculture. ResultsLiposomal VD3 primed-moDCs induced the development of regulatory CD4+ T cells (Tregs) that inhibited bystander memory T cell proliferation. Induced Tregs were of the FoxP3+ CD127low phenotype, also expressing TIGIT. Additionally, liposome-VD3 primed moDCs inhibited the development of T helper 1 (Th1) and T helper 17 (Th17) cells. Skin injection of VD3 liposomes selectively stimulated the migration of CD14+ skin DCs. DiscussionThese results suggest that nanoparticulate VD3 is a tolerogenic tool for DC-mediated induction of regulatory T cell responses.