bluuebunny/crossref_metadata_2025_split huggingface dataset:
Cuproptosis-related LncRNAs are potential prognostic and immune response markers for patients with HNSCC via the integration of bioinformatics analysis and experimental validation
Authors: Zhou Liuqing , Cheng Qing , Hu Yao , Tan Haoyue , Li Xiaoguang , Wu Shuhui , Zhou Tao , Zhou Jieyu 
Published: December 2022

IntroductionHead and neck squamous cell carcinoma (HNSCC) is a malignant neoplasm typically induced by alcohol and tobacco consumption, ranked the sixth most prevalent cancer globally. This study aimed to establish a cuproptosis-related lncRNA predictive model to assess the clinical significance in HNSCC patients.MethodsThe Cancer Genome Atlas (TCGA) database was utilized to download cuproptosis-related genes, lncRNAs profiles, and selected clinical information of 482 HNSCC samples. Cuproptosis-related lncRNAs were analyzed by Pearson correlation method, with the least absolute shrinkage and selection operator (LASSO) and univariate/multivariate Cox analyses performed to establish the cuproptosis-related lncRNA predictive model. Subsequently, the time-dependent receiver operating characteristics (ROC) and Kaplan-Meier analysis were applied to assess its prediction ability, and the model was verified by a nomogram, univariate/multivariate Cox analysis, and calibration curves. Furthermore, the principal component analysis (PCA), immune analysis, and gene set enrichment analyses (GSEA) were performed, and the 50% inhibitory concentration (IC50) prediction in the risk groups was calculated. Furthermore, the expression of six cuproptosis-related lncRNAs in HNSCC and paracancerous tissues was detected by quantitative real-time PCR (qRT-PCR).ResultsA total of 467 lncRNAs were screened as cuproptosis-associated lncRNAs in HNSCC tissues to establish an eight cuproptosis-related lncRNA prognostic signature consisting of AC024075.3, AC090587.2, AC116914.2, AL450384.2, CDKN2A-DT, FAM27E3, JPX, and LNC01089. For the high-risk group, the results demonstrated a satisfactory predicting performance with considerably worse overall survival (OS). Multivariate Cox regression confirmed that the risk score was a reliable predictive factor (95% CI: 1.089–1.208, hazard ratio =1.147), with the area of 1-, 3-, and 5-year OS under the ROC curve of 0.690, 0.78524, and 0.665, respectively. The differential analysis revealed that JPX was significantly upregulated in HNSCC tissues, while AC024075.3, AC090587.2, AC116914.2, AL450384.2, CDKN2A-DT were downregulated in HNSCC tissues by qRT-PCR assays. In addition, this gene signature was also associated with some immune-related pathways and immune cell infiltration and affected the anti-cancer immune response. Furthermore, Bexarotene, Bleomycin, Gemcitabine, etc., were identified as potential therapeutic compounds for HNSCC.DiscussionsThis novel cuproptosis-related lncRNAs prognostic signature could predict prognosis and help propose novel individual therapeutic targets for HNSCC.
Tumour microenvironment landscape and immunotherapy response in bladder cancer decoded by stromal MOXD1 based on copper-related genes signature
Authors: Wang Wenhao , Hua Shan , Li Jianying , Zhao Jing , Zhang Yu , Jiang Juntao , Han Bangmin 
Published: December 2022

IntroductionWe aimed to develop a copper-related gene (CRG) signature that can be used to evaluate prognosis and guide therapeutic management in bladder cancer patients.MethodsThe raw transcriptome profiles and clinical data of 405 bladder samples were downloaded from The Cancer Genome Atlas (TCGA) database, and differentially expressed copper-related genes were identifified using the Molecular Signatures Database (MSigDB) database and univariate and multivariate Cox regression analysis. A multigene prognostic signature based on 14 CRGs was developed by least absolute shrinkage and selection operation (LASSO) analysis in the TCGA cohort and validated in the Gene Expression Omnibus (GEO) cohort. Multiple analyses were then conducted in which the nomograms, clinicopathological features, immune-related cell infifiltration characteristics, and therapy responses of the high- and low-risk score groups were compared.ResultsA 14 CRGs signature was constructed and used to classify patients into high-risk and low-risk groups. Compared to patients classifified as high-risk, low-risk patients in both the TCGA cohort and the GEO cohort had better overall survival. Patients in high-risk groups had more aggressive clinical features, immunologically “cold” infifiltrating characteristics, and experienced lower therapeutic effificacy. We identifified a CRG signature of bladder cancer and validated it using unsupervised clustering analysis. Monooxygenase DBH-like 1 (MOXD1) was further identifified, and its potential for evaluating the tumor immune microenvironment and predicting the immunotherapy response was explored.DiscussionThese results suggest a novel research direction for precision therapy of bladder cancer and demonstrate that copper-related genes can play a promising role in predicting prognosis and may serve as therapeutic targets for bladder cancer.
Association of genetic ancestry with HER2, GRB7 AND estrogen receptor expression among Colombian women with breast cancer
Authors: Rey-Vargas Laura , Bejarano-Rivera Lina María , Mejia-Henao Juan Carlos , Sua Luz F. , Bastidas-Andrade Jhon Faustino , Ossa Carlos Andrés , Gutiérrez-Castañeda Luz Dary , Fejerman Laura , Sanabria-Salas María Carolina , Serrano-Gómez Silvia J. 
Published: December 2022

BackgroundOur previous study reported higher mRNA levels of the human epidermal growth factor receptor 2 (HER2)-amplicon genes ERBB2 and GRB7 in estrogen receptor (ER)-positive breast cancer patients with relatively high Indigenous American (IA) ancestry from Colombia. Even though the protein expression of HER2 and GRB7 is highly correlated, they may also express independently, an event that could change the patients’ prognosis. In this study, we aimed to explore the differences in ER, HER2 and GRB7 protein expression according to genetic ancestry, to further assess the clinical implications of this association.MethodsWe estimated genetic ancestry from non-tumoral breast tissue DNA and assessed tumoral protein expression of ER, HER2, and GRB7 by immunohistochemistry in a cohort of Colombian patients from different health institutions. We used binomial and multinomial logistic regression models to test the association between genetic ancestry and protein expression. Kaplan-Meier and log-rank tests were used to evaluate the effect of HER2/GRB7 co-expression on patients’ survival.ResultsOur results show that patients with higher IA ancestry have higher odds of having HER2+/GRB7- breast tumors, compared to the HER2-/GRB7- subtype, and this association seems to be stronger among ER-positive tumors (ER+/HER2+/GRB7-: OR=3.04, 95% CI, 1.47-6.37, p&lt;0.05). However, in the multivariate model this association was attenuated (OR=1.80, 95% CI, 0.72-4.44, p=0.19). On the other hand, it was observed that having a higher European ancestry patients presented lower odds of ER+/HER2+/GRB7- breast tumors, this association remained significant in the multivariate model (OR=0.36, 95% CI, 0.13 - 0.93, p= 0.0395). The survival analysis according to HER2/GRB7 co-expression did not show statistically significant differences in the overall survival and recurrence-free survival.ConclusionsOur results suggest that Colombian patients with higher IA ancestry and a lower European fraction have higher odds of ER+/HER2+/GRB7- tumors compared to ER+/HER2-/GRB7- disease. However, this association does not seem to be associated with patients’ overall or recurrence-free survival.
Bibliometric analysis on exploitation of biogenic gold and silver nanoparticles in breast, ovarian and cervical cancer therapy
Authors: Bhandari Meena , Raj Seema , Kumar Ashwani , Kaur Dilraj Preet 
Published: December 2022

Multifunctional nanoparticles are being formulated to overcome the side effects associated with anticancer drugs as well as conventional drug delivery systems. Cancer therapy has gained the advancement due to various pragmatic approaches with better treatment outcomes. The metal nanostructures such as gold and silver nanoparticles accessible via eco-friendly method provide amazing characteristics in the field of diagnosis and therapy towards cancer diseases. The environmental friendly approach has been proposed as a substitute to minimize the use of hazardous compounds associated in chemical synthesis of nanoparticles. In this attempt, researchers have used various microbes, and plant-based agents as reducing agents. In the last 2 decades various papers have been published emphasizing the benefits of the eco-friendly approach and advantages over the traditional method in the cancer therapy. Despite of various reports and published research papers, eco-based nanoparticles do not seem to find a way to clinical translation for cancer treatment. Present review enumerates the bibliometric data on biogenic silver and gold nanoparticles from Clarivate Analytics Web of Science (WoS) and Scopus for the duration 2010 to 2022 for cancer treatment with a special emphasis on breast, ovarian and cervical cancer. Furthermore, this review covers the recent advances in this area of research and also highlights the obstacles in the journey of biogenic nanodrug from clinic to market.
Randomized controlled trial of emotion-focused training for helping professionals
Authors: Halamová Júlia , Ondrejková Natália , Kováč Karol 
Published: December 2022

IntroductionThe aim of this study was to examine the short- and long-term effectiveness of the novel Emotion-focused Training for Helping Professions on levels of compassion fatigue (secondary traumatic stress &amp; burnout), self-criticism, self-compassion, and compassion for others.MethodsA randomized controlled trial study was conducted. A total of 253 participants were recruited and randomly assigned to either the experimental group or the control group. The experimental group attended a 14-day online training. The control group did not perform any tasks.ResultsResults showed that after completing the intervention the experimental group participants reported significantly lower scores for secondary traumatic stress, burnout and self-criticism, and higher scores for self-compassion and that these lasted for two months after completion. Compared to the control group, the experimental group participants had significantly lower scores of secondary traumatic stress, burnout, self-criticism, and higher scores of self-compassion after the intervention. No significant changes were found for the control group, except a significant increase in time in the reported score for one dimension of burnout – exhaustion.DiscussionThe novel EFT-HP training was shown to be effective in reducing levels of compassion fatigue (secondary traumatic stress and burnout) and self-criticism and increasing self-compassion.
Self-neglect among older adults with disabilities in Liaoning Province during the COVID-19 outbreak: A cross-sectional study
Authors: Dong Li , Sun Longfeng 
Published: December 2022

BackgroundSelf-neglect among older adults is a well-recognized public health issue. During the novel coronavirus disease 2019 (COVID-19), older adults with disabilities may be at increased risk for self-neglect. This study aimed to assess the prevalence of self-neglect and associated factors among older adults with disabilities in Liaoning Province during the COVID-19 outbreak.MethodsA cross-sectional study was conducted from January 2021 to April 2021. A convenience sample of 230 older adults with disabilities filled out questionnaires that collected data on socioeconomic and demographic characteristics alongside data using the Barthel Index (BI), the Elder Self-Neglect Assessment (ESNA), the Geriatric Depression Scale-Short Form (GDS-SF), the Multidimensional Scale of Perceived Social Support (MSPSS), and the Mini-Mental State Examination (MMSE).ResultsThe overall self-neglect rate was 86%. The study revealed that self-neglect correlates positively with the degree of disability (p &lt; 0.001) and depressive symptoms (p &lt; 0.001). In contrast, monthly income (p = 0.002) and perceived social support (p = 0.001) were negatively associated with self-neglect.ConclusionDuring the COVID-19 outbreak, self-neglect has been a serious public health problem among older adults with disabilities. Lower monthly income, decreased physical function, depressive symptoms, and perceptions of poor social support contributed to self-neglect among older adults with disabilities.
Mining of risk perception dimensions of Chinese tourists’ outbound tourism based on word vector method
Authors: Yu Caihua , Zhao Yilin 
Published: December 2022

IntroductionSafety is the lifeline of tourism development. The article’s goal is to examin how Chinese tourists perceive risk when travelling aboard.MethodsIn order to create the initial corpus, this study first uses “outbound tourism“as the key word to crawl the question and answer (hereinafter referred to as “Q &amp; A”) data from 4 Chinese online travel operator platforms, then preprocesses the “Q &amp; A” data in Python. Secondly, after being extracted, the feature words are converted into the word vector model using the word vector method based on neural network language model. Finally, the word vectors are clustered and classified.ResultsIt is found that there are six dimensions of risk perception of Chinese tourists’ outbound tourism, namely traffic risk, planning risk, service risk, communication risk, financial risk and functional risk.DiscussionImportant and practical information for government and tourism enterprises is provided to accurately grasp the risk perception of Chinese tourists’ outbound tourism and continuously improve the supply of tourism risk information.
The associations of COVID-19 percent positivity rate, relationship quality, and season with daily anxiety and depression in couples living in NYC
Authors: Cornelius Talea , DiGiovanni Ana M. , Bolger Niall 
Published: December 2022

BackgroundThe COVID-19 pandemic changed nearly every aspect of daily life and had detrimental effects on mental health. Yet, impacts have been heterogeneous. We tested whether fluctuations in local COVID-19 percent positivity rates were associated with daily anxiety and depression in couples living in NYC, as well as whether these associations varied by relationship quality or season. We expected that adverse impacts of COVID-19 may be attenuated by high-quality relationships and during warmer months, or that people may habituate over time.MethodsData on seven-day rolling average COVID-19 percent positive rate each day in NYC were merged with a 14-day dyadic diary study of cohabiting couples living in NYC between August 2020 through April 2021 (232 individuals from 116 couples; mean age 28.42 years, 52.59% female, 53.02% White). Dyadic multilevel models estimated the association COVID-19 positivity rate, season (sine and cosine of the calendar date), baseline relationship quality, and all two-and three-way interactions of these variables with daily anxiety and depression. Covariates included weekend and COVID-positive case within the couple.ResultsAnxiety and depression mirrored COVID-19 positivity rates, and there was some evidence for habituation over time. Significant two-and three-way interactions suggested that being in a high-quality relationship buffered the association of COVID-19 positivity rate with both anxiety and depression during months when cases were low. Anxiety was elevated for individuals in high- (v. low-) quality relationships during the December–January surge.ConclusionSeven-day rolling average COVID-19 percent positivity rate was associated with daily anxiety and depression among couples living in NYC. There was some evidence that individuals habituated to this stressor over time and that high-quality relationships were protective for mental well-being; however, there was some suggestion that couples in high-quality relationships may have engaged in processes such as co-rumination during surges, worsening their daily anxiety.
Follow-up study of depressive state on patients with atrial fibrillation 1 year after radio-frequency ablation
Authors: Ren Lei , Li Wenjun , Su Xin , Yang Yangyang , Zhang Yuanzhuo , Liu Xiaozhu , Hu Guangquan , Ning Bin 
Published: December 2022

ObjectiveTo analyze the effect of depression on the recurrence of atrial fibrillation (AF) 1 year after radio-frequency ablation.MethodsA total of 91 patients with AF admitted to our hospital from January 2020 to July 2021 were studied. All patients were followed up 1 year after radio-frequency ablation. A total of 91 subjects were divided into recurrence group (n = 30) and no recurrence group (n = 61) according to the recurrence situation 1 year after radio-frequency ablation. Age, disease course, body mass index (BMI), gender, echocardiography (left atrial diameter), blood inflammatory indicators (neutrophil count, lymphocyte count, and monocyte count), and Self-rating Depression Scale (SDS) scores were compared between the two groups. Logistic multivariate regression analysis was used to analyze the effect of SDS score and other indexes on the recurrence of AF 1 year after radio-frequency ablation.ResultsThe age of patients in relapse group was higher than that in no relapse group (P &lt; 0.05) and the course of disease was longer than that of the no recurrence group (P &lt; 0.05). The BMI was higher than that of the no recurrence group (P &lt; 0.05) and the left atrial diameter was greater than that of the no recurrence group (P &lt; 0.05). Neutrophil count and monocyte count were significantly higher than those in no recurrence group (P &lt; 0.05) and the lymphocyte count was significantly lower than that in the no recurrence group (P &lt; 0.05). There were significant differences in SDS score composition between the two groups (P &lt; 0.05) and the composition ratio of patients with moderate and major depression in the relapsing group was significantly higher than that in the non-relapsing group. The composition ratio of patients without depression in the relapsing group was significantly lower than that in the non-relapsing group. Multivariate analysis showed that age, disease course, BMI, left atrial diameter, neutrophil count, lymphocyte count, monocyte count, and SDS score were all independent factors affecting the recurrence of AF patients 1 year after radio frequency ablation (P &lt; 0.05). Compared with patients without depression, patients with mild, moderate and major depression had an increased risk of recurrence by 74.0, 98.2, and 151.2% 1 year after radio-frequency ablation, respectively (OR = 1.740, 1.982, and 2.512).ConclusionThere is a high rate of depression in patients with AF. Depression is an important factor affecting the early recurrence of patients with AF after radio-frequency ablation.
The role of mind body interventions in the treatment of irritable bowel syndrome and fibromyalgia
Authors: Islam Zarmina , D’Silva Adrijana , Raman Maitreyi , Nasser Yasmin 
Published: December 2022

IntroductionIrritable bowel syndrome and fibromyalgia share similar pathophysiologic mechanisms including sensitization of peripheral and central pain pathways, autonomic dysfunction and are often co-diagnosed. Co-diagnosed patients experience increased symptom severity, mental health comorbidities, and decreased quality of life. The role of mind-body interventions, which have significant effects on central pain syndromes and autonomic dysregulation, have not been well-described in co-diagnosed patients. The aim of this state-of-the art narrative review is to explore the relationship between irritable bowel syndrome and fibromyalgia, and to evaluate the current evidence and mechanism of action of mind-body therapies in these two conditions.MethodsThe PubMed database was searched without date restrictions for articles published in English using the following keywords: fibromyalgia, irritable bowel syndrome, mind-body interventions, cognitive behavioral therapy, mindfulness based stress reduction, and yoga.ResultsMind-body interventions resulted in improved patient-reported outcomes, and are effective for irritable bowel syndrome and fibromyalgia individually. Specifically, cognitive behavioral therapy and yoga trials showed decreased symptom severity, improved mental health, sleep and quality of life for both conditions individually, while yoga trials demonstrated similar benefits with improvements in both physical outcomes (gastrointestinal symptoms, pain/tenderness scores, insomnia, and physical functioning), mental health outcomes (anxiety, depression, gastrointestinal-specific anxiety, and catastrophizing), and quality of life, possibly due to alterations in autonomic activity.ConclusionMind-body interventions especially CBT and yoga improve patient-reported outcomes in both irritable bowel syndrome and fibromyalgia individually. However, limited available data in co-diagnosed patients warrant high quality trials to better tailor programs to patient needs.
Healthcare system development in the Middle East and North Africa region: Challenges, endeavors and prospective opportunities
Authors: Katoue Maram Gamal , Cerda Arcadio A. , García Leidy Y. , Jakovljevic Mihajlo 
Published: December 2022

BackgroundCountries in the Middle East and North Africa (MENA) region have been investing in the development of their health systems through implementing reforms to improve health care delivery for their nations. However, these countries are still facing challenges in providing equitable, high quality healthcare services. There is limited published literature supporting the previous and ongoing attempts that have been made to improve health system performance in MENA countries.AimsThis review aims to describe experiences of health system development efforts in the MENA region, highlight progress, identify challenges that need be addressed and future opportunities to achieve responsive and efficient health systems. It also aimed to provide recommendations to further support these health systems toward evolution and performance improvement.MethodsA literature review was conducted by searching different databases including PubMed, Scopus, Google Scholar and other electronic resources to identify articles and publications describing health systems development in the MENA region from 1975 to 2022. It also included grey literature, reports and policy and planning documents by international organizations. The identified references were reviewed to extract, analyze, organize and report the findings.ResultsThe review revealed emerging evidence describing governmental initiatives to introduce health system reforms at different levels in the MENA countries. These include initiatives targeting the various elements controlling health system reform: financing, payment, organization, regulation and behavior of providers and consumers. There are several challenges facing the health systems of MENA countries including the rising burden of chronic diseases, inequitable access to health services, deficiency in health workforce, shortage in the use of effective health information systems and leadership challenges. The review identified several key areas that can benefit from further improvement to support health system reforms. These include improving the structure, organization and financing of health systems, health workforce development, effective data management and engagement of key stakeholders to achieve adequate health system reforms.ConclusionThe MENA countries have made significant steps to improve the performance of their health systems; yet achieving a comprehensive health reform will require collaboration of various stakeholders including health policy makers, healthcare professionals, and central to the success of the reform, the patients.