bluuebunny/crossref_metadata_2025_split huggingface dataset:
Forward genetic screen of Caenorhabditis elegans mutants with impaired sleep reveals a crucial role of neuronal diacylglycerol kinase DGK-1 in regulating sleep
Authors: Chen Chung-Kuan , Kawano Taizo , Yanagisawa Masashi , Hayashi Yu 
Published: September 2023

Abstract
The sleep state is widely observed in animals. The molecular mechanisms underlying sleep regulation, however, remain largely unclear. In the nematode Caenorhabditis elegans, developmentally timed sleep (DTS) and stress-induced sleep (SIS) are 2 types of quiescent behaviors that fulfill the definition of sleep and share conserved sleep-regulating molecules with mammals. To identify novel sleep-regulating molecules, we conducted an unbiased forward genetic screen based on DTS phenotypes. We isolated 2 mutants, rem8 and rem10, that exhibited significantly disrupted DTS and SIS. The causal gene of the abnormal sleep phenotypes in both mutants was mapped to dgk-1, which encodes diacylglycerol kinase. Perhaps due to the diminished SIS, dgk-1 mutant worms exhibited decreased survival following exposure to a noxious stimulus. Pan-neuronal and/or cholinergic expression of dgk-1 partly rescued the dgk-1 mutant defects in DTS, SIS, and post-stress survival. Moreover, we revealed that pkc-1/nPKC participates in sleep regulation and counteracts the effect of dgk-1; the reduced DTS, SIS, and post-stress survival rate were partly suppressed in the pkc-1; dgk-1 double mutant compared with the dgk-1 single mutant. Excessive sleep observed in the pkc-1 mutant was also suppressed in the pkc-1; dgk-1 double mutant, implying that dgk-1 has a complicated mode of action. Our findings indicate that neuronal DGK-1 is essential for normal sleep and that the counterbalance between DGK-1 and PKC-1 is crucial for regulating sleep and mitigating post-stress damage.
Mpox-Related Ophthalmic Disease: A Retrospective Observational Study in a Single Center in Mexico
Authors: Rodríguez-Badillo Paulina , Rodríguez-Aldama Juan Carlos , Gabián-Fortes Lucía del Carmen , Sifuentes-Rentería Sergio , Valdez-González María Teresa , Pérez-Flores Brian Eduardo , Velasco-Ramos Regina , Fernández-Vizcaya Oscar , Crabtree-Ramírez Brenda , Pérez-Barragán Edgar 
Published: September 2023

Abstract
Mpox-related ophthalmic disease has been reported as infrequent. We retrospectively describe the ocular manifestations present in 11 of 100 patients with confirmed mpox; 9 were people with HIV. We suggest that an ophthalmological evaluation should be performed in all patients with ocular symptoms or moderate and severe mpox disease.
Prolonged use of linezolid in bone and joint infections: a retrospective analysis of adverse effects
Authors: Veerman Karin , Goosen Jon , Spijkers Karin , Jager Nynke , Heesterbeek Petra , Telgt Denise 
Published: September 2023

Abstract

Objectives
Antibiotic treatment for bone and joint infections generally lasts for 6 weeks or longer. Linezolid may be a good option for treating bone and joint infections, but there is an increased risk of potential serious adverse drug events (ADEs) when used for more than 28 days. The aim of this study was to obtain detailed information on the type and time to occurrence of the patient-reported ADEs, the dynamics of haematopoiesis over time, and the reasons for early discontinuation of linezolid when used for an intended maximum duration of 12 weeks.

Methods
This single-centre retrospective study was conducted at the Sint Maartenskliniek in The Netherlands. Patients were included if they were planned to use linezolid for more than 28 days. The main reason for discontinuation of linezolid, the ADE according to the Naranjo score, and the time to occurrence of ADEs were analysed.

Results
Among 78 patients, drug toxicity led to early discontinuation of linezolid in 11 (14%) patients before and nine (12%) after 28 days of therapy. The median treatment duration was 42 days. Gastrointestinal intolerance (42%) and malaise (32%) were the most common ADEs. In 75% of the cases the ADE occurred within 28 days of therapy. Sixty-seven patients were able to continue linezolid beyond 28 days, 87% of whom completed therapy as scheduled. Severe cytopenia, according to the Common Terminology Criteria for Adverse events (CTCA), was observed in four patients and was reversible after discontinuation of linezolid. One patient suffered optic neuropathy related to linezolid use.

Conclusions
Linezolid could be considered an alternative option to the current standard of IV glycopeptides for the treatment of bone and joint infection for up to 12 weeks. If patients pass the first 28 days of therapy, the likelihood of successful completion of therapy is high with a low risk of serious ADEs.
Whole-genome resequencing reveals the genomic diversity and signatures of selection in Romanov sheep
Authors: Cao Chunna , Kang Yuxin , Zhou Qian , Nanaei Hojjat Asadollahpour , Bo Didi , Liu Peiyao , Bai Yangyang , Li Ran , Jiang Yu , Lan Xianyong , Pan Chuanying 
Published: September 2023

Abstract
Romanov sheep are adapted to the extremely cold and harsh environment and display a distinctive grey color. Herein, we analyzed the population structure, genetic diversity, and selection signatures of Romanov sheep based on whole-genome sequencing data of 17 Romanov sheep, 114 individuals from other 10 European breeds. The results of PCA, ADMIXTURE, and NJ-tree showed that the Romanov sheep was closely related to other northern European breeds. A relative high level of genetic diversity, low inbreeding coefficient, and large effective population size was observed in Romanov sheep when compared with other European breeds. We then screened the genomic selection signatures of Romanov sheep using FST, XP-XLP, and XP-EHH methods. The most significant region under selection (CHR14:14.2 to 14.3 Mb) harbored a haplotype that contained MC1R gene. Furthermore, this haplotype was also found in other grey-body breeds including Gotland sheep, Grey Tronder Sheep, and German grey heath sheep, suggesting that it was associated with the unique coat color of these breeds. We also found one region (CHR10:40.8Mb- 41.0Mb) harboring PCDH9 gene which was potentially associated with cold environmental adaptation. In summary, this study identified candidate genes that were associated with the unique grey color and environmental adaptation in Romanov sheep, which provided a basis for understanding the genetic background and utilization of this breed.
Influence of handling in corrals on the temperament of different breeds of beef cattle raised in Brazil
Authors: Freitas Anielly de P , Lima Maria Lúcia P , Simili Flávia F , Negrão João A , Schenkel Flavio S , de Paz Claudia Cristina P 
Published: September 2023

Abstract
The objective of the present study was to verify the effect of handling in corrals on the stress of beef cattle of Nelore, Caracu, and Guzerá breeds through the analysis of the relationship between behavioral traits, growth, and blood parameters. A total of 778 records of entry scores, chute scores, exit scores, flight speed, cortisol, glucose, lactate, live weight, and body condition scores were collected on steers and heifers of the three breeds, born between September and December. The animals came from the Advanced Beef Cattle Research Center of the Animal Science Institute. The variance analyses were performed using the GENMOD procedure. The PROC CORR estimated Spearman correlation coefficients for all traits studied, and the principal components analysis was performed according to the procedure PRINCOMP. Heifers had higher reactivity than steers, with higher behavioral scores and blood parameters. Overall, Caracu individuals demonstrated lower reactivity in comparison to Nelore and Guzerá. However, they presented high stress when they were restrained. The principal component analysis highlighted that behavioral traits and blood parameters were contrasted with growth traits; thus, this analysis can help selectors (livestock farmers) understand the importance of selecting less reactive animals and more easily choose the animals they want to select for breeding. Our results indicate that handling in corrals could be considered a stressful event for bovines. For this reason, measuring blood parameters and behavioral traits can compose necessary decision-making tools regarding changes and adequacies while handling events to reduce stress and consequently improve the herd’s reactivity.
Heat shock protein 27 regulates myogenic and self-renewal potential of bovine satellite cells under heat stress
Authors: Kim Won Seob , Daddam Jayasimha R , Keng Boon Hong , Kim Jaehwan , Kim Jongkyoo 
Published: September 2023

Abstract
While satellite cells play a key role in the hypertrophy, repair, and regeneration of skeletal muscles, their response to heat exposure remains poorly understood, particularly in beef cattle. This study aimed to investigate the changes in the transcriptome, proteome, and proliferation capability of bovine satellite cells in response to different levels of heat stress (HS) and exposure times. Satellite cells were isolated from 3-mo-old Holstein bulls (body weight: 77.10 ± 2.02 kg) and subjected to incubation under various temperature conditions: 1) control (38 °C; CON), 2) moderate (39.5 °C; MHS), and extreme (41 °C; EHS) for different durations ranging from 0 to 48 h. Following 3 h of exposure to extreme heat (EHS), satellite cells exhibited significantly increased gene expression and protein abundance of heat shock proteins (HSPs; HSP70, HSP90, HSP20) and paired box gene 7 (Pax7; P &lt; 0.05). HSP27 expression peaked at 3 h of EHS and remained elevated until 24 h of exposure (P &lt; 0.05). In contrast, the expression of myogenic factor 5 (Myf5) and paired box gene 3 (Pax3) was decreased by EHS compared to the control at 3 h of exposure (P &lt; 0.05). Notably, the introduction of HSP27 small interference RNA (siRNA) transfection restored Myf5 expression to control levels, suggesting an association between HSP27 and Myf5 in regulating the self-renewal properties of satellite cells upon heat exposure. Immunoprecipitation experiments further confirmed the direct binding of HSP27 to Myf5, supporting its role as a molecular chaperone for Myf5. Protein–protein docking algorithms predicted a high probability of HSP27–Myf5 interaction as well. These findings indicate that extreme heat exposure intrinsically promotes the accumulation of HSPs and modulates the early myogenic regulatory factors in satellite cells. Moreover, HSP27 acts as a molecular chaperone by binding to Myf5, thereby regulating the division or differentiation of satellite cells in response to HS. The results of this study provide a better understanding of muscle physiology in heat-stressed cells, while unraveling the intricate molecular mechanisms that underlie the HS response in satellite cells.
Risk stratification of invasive cervical cancer diagnosed after cervical conization
Authors: Shiga Tomoka , Taguchi Ayumi , Mori Mayuyo , Yamaguchi Shogo , Honjoh Harunori , Nishijima Akira , Eguchi Satoko , Miyamoto Yuichiro , Sone Kenbun , Kawana Kei , Osuga Yutaka 
Published: September 2023

Abstract

Background
Cervical intraepithelial neoplasia (CIN) diagnosis is based on colposcopy-aided histological examination. However, its accuracy in CIN diagnosis is limited. Some invasive cervical cancers (ICCs) are diagnosed after cervical conization. Therefore, risk stratification of undetected ICC is particularly important for the management of patients with CIN. This study aimed to identify the risk factors for undetected ICC. We especially focused on the association of human papillomavirus (HPV) genotypes.

Methods
We retrospectively reviewed the clinicopathological characteristics (including age, parity, and preoperative diagnosis) and HPV genotypes of 348 patients diagnosed with CIN or adenocarcinoma in situ (AIS) who underwent cervical conization at our hospital between 2008 and 2016. The relationship between preoperative factors, including HPV genotypes and post-conization ICC, was evaluated.

Results
Among the 348 patients, 322, 7, and 19 had preoperative CIN3, CIN2, and AIS, respectively; 181 were nulliparous. The median patient age was 41 (23–83) years. HPV genotyping was performed on 237 patients. Overall, post-conization ICC was detected in 16 patients (4.6%). Multivariate analysis showed that nulliparity and HPV16 positivity were the independent risk factors for post-conization ICC (OR: 6.01, P = 0.0302; OR: 5.26, P = 0.0347, respectively). The combination of HPV16 status and parity improved diagnostic accuracy. Seven of 53 HPV16-positive cases (13%) without childbirth history were diagnosed with post-conization ICC. In contrast, none of the HPV16-negative cases with childbirth history was diagnosed with post-conization ICC.

Conclusion
HPV16 positivity and nulliparity were identified as risk factors for undetected ICC. Careful treatment selection and preoperative scrupulous examination are especially important in these cases.
Israel, an Anatomy of the World: Microcosmic Contours Between Romans 9–11 and 1–8
Authors: Dixon Thomas P 
Published: September 2023

Abstract
While a near consensus now deems Romans 9–11 integral to the letter rather than peripheral, explanations of how these chapters integrate remain disparate. Apparent links between Rom. 9–11 and 1–8 abound, but no one has yet exposed the underlying congruence between the two sections’ argumentative trajectories. Coordinating divine activity in these letter segments reveals the interpretive value of ‘microcosm’ imagery, an ancient and versatile metaphor that will prove more illuminating than ‘climax’ inter alia for understanding Paul’s presentation of Israel. In Romans, the κόσμος itself is an actor in the text, with Israel serving as its microcosm. Romans 9–11 then portrays God’s actions toward Israel in a microcosmic relation to God’s actions toward the κόσμος in Rom. 1–8. In its disobedience, judgement, and salvation, Israel embodies the anatomy of the world, a layered harmony that may help reconcile warring camps in recent Pauline scholarship.
Drought survival in conifer species is related to the time required to cross the stomatal safety margin
Authors: Petek-Petrik Anja , Petrík Peter , Lamarque Laurent J , Cochard Hervé , Burlett Régis , Delzon Sylvain 
Published: September 2023

Abstract
The regulation of water loss and the spread of xylem embolism have mostly been considered separately. The development of an integrated approach taking into account the temporal dynamics and relative contributions of these mechanisms to plant drought responses is urgently needed. Do conifer species native to mesic and xeric environments display different hydraulic strategies and temporal sequences under drought? A dry-down experiment was performed on seedlings of four conifer species differing in embolism resistance, from drought-sensitive to extremely drought-resistant species. A set of traits related to drought survival was measured, including turgor loss point, stomatal closure, minimum leaf conductance, and xylem embolism resistance. All species reached full stomatal closure before the onset of embolism, with all but the most drought-sensitive species presenting large stomatal safety margins, demonstrating that highly drought-resistant species do not keep their stomata open under drought conditions. Plant dry-down time to death was significantly influenced by the xylem embolism threshold, stomatal safety margin, and minimum leaf conductance, and was best explained by the newly introduced stomatal margin retention index (SMRIΨ50) which reflects the time required to cross the stomatal safety margin. The SMRIΨ50 may become a key tool for the characterization of interspecific drought survival variability in trees.
On the fundamentality of the radial acceleration relation for late-type galaxy dynamics
Authors: Stiskalek Richard , Desmond Harry 
Published: September 2023

ABSTRACT
Galaxies have been observed to exhibit a level of simplicity unexpected in the complex galaxy formation scenario posited by standard cosmology. This is particularly apparent in their dynamics, where scaling relations display much regularity and little intrinsic scatter. However, the parameters responsible for this simplicity have not been identified. Using the Spitzer Photometry & Accurate Rotation Curves galaxy catalogue, we argue that the radial acceleration relation (RAR) between galaxies’ baryonic and total dynamical accelerations is the fundamental 1D correlation governing the radial (in-disc) dynamics of late-type galaxies. In particular, we show that the RAR cannot be tightened by the inclusion of any other available galaxy property, that it is the strongest projection of galaxies’ radial dynamical parameter space, and that all other statistical radial dynamical correlations stem from the RAR plus the non-dynamical correlations present in our sample. We further provide evidence that the RAR’s fundamentality is unique in that the second most significant dynamical relation does not possess any of these features. Our analysis reveals the root cause of the correlations present in galaxies’ radial dynamics: they are nothing but facets of the RAR. These results have important ramifications for galaxy formation theory because they imply that to explain statistically late-type galaxy dynamics within the disc it is necessary and sufficient to explain the RAR and lack of any significant, partially independent correlation. While simple in some modified dynamics models, this poses a challenge to standard cosmology.
Probing the Galactic halo with RR Lyrae stars – IV. On the Oosterhoff dichotomy of RR Lyrae stars
Authors: Zhang Shan , Liu Gaochao , Huang Yang , Lv Zongfei , Bird Sarah Ann , Chen Bingqiu , Zhang Huawei , Beers Timothy C , Li Xinyi , Tian Haijun , Zhang Peng 
Published: September 2023

ABSTRACT
We use 3653 (2661 RRab, 992 RRc) RR Lyrae stars (RRLs) with 7D (3D position, 3D velocity, and metallicity) information selected from Sloan Digital Sky Survey, Large Sky Area Multi-Object Fiber Spectroscopic Telescope, and Gaia EDR3, and divide the sample into two Oosterhoff groups (Oo I and Oo II) according to their amplitude–period behaviour in the Bailey diagram. We present a comparative study of these two groups based on chemistry, kinematics, and dynamics. We find that Oo I RRLs are relatively more metal-rich, with predominately radially dominated orbits and large eccentricities, while Oo II RRLs are relatively more metal-poor, and have mildly radially dominated orbits. The Oosterhoff dichotomy of the Milky Way’s halo is more apparent for the inner-halo region than for the outer-halo region. Additionally, we also search for this phenomenon in the haloes of the two largest satellite galaxies, the Large and Small Magellanic clouds, and compare over different bins in metallicity. We find that the Oosterhoff dichotomy is not immutable, and varies based on position in the Galaxy and from galaxy to galaxy. We conclude that the Oosterhoff dichotomy is the result of a combination of stellar and galactic evolution, and that it is much more complex than the dichotomy originally identified in Galactic globular clusters.
The MillenniumTNG Project: semi-analytic galaxy formation models on the past lightcone
Authors: Barrera Monica , Springel Volker , White Simon D M , Hernández-Aguayo César , Hernquist Lars , Frenk Carlos , Pakmor Rüdiger , Ferlito Fulvio , Hadzhiyska Boryana , Delgado Ana Maria , Kannan Rahul , Bose Sownak 
Published: September 2023

ABSTRACT
Upcoming large galaxy surveys will subject the standard cosmological model, Lambda Cold Dark Matter, to new precision tests. These can be tightened considerably if theoretical models of galaxy formation are available that can predict galaxy clustering and galaxy–galaxy lensing on the full range of measurable scales, throughout volumes as large as those of the surveys, and with sufficient flexibility that uncertain aspects of the underlying astrophysics can be marginalized over. This, in particular, requires mock galaxy catalogues in large cosmological volumes that can be directly compared to observation, and can be optimized empirically by Monte Carlo Markov Chains or other similar schemes, thus eliminating or estimating parameters related to galaxy formation when constraining cosmology. Semi-analytic galaxy formation methods implemented on top of cosmological dark matter simulations offer a computationally efficient approach to construct physically based and flexibly parametrized galaxy formation models, and as such they are more potent than still faster, but purely empirical models. Here, we introduce an updated methodology for the semi-analytic L-Galaxies code, allowing it to be applied to simulations of the new MillenniumTNG project, producing galaxies directly on fully continuous past lightcones, potentially over the full sky, out to high redshift, and for all galaxies more massive than $\sim 10^8\, {\rm M}_\odot$. We investigate the numerical convergence of the resulting predictions, and study the projected galaxy clustering signals of different samples. The new methodology can be viewed as an important step towards more faithful forward-modelling of observational data, helping to reduce systematic distortions in the comparison of theory to observations.
H i-rich but low star formation galaxies in MaNGA: physical properties and comparison to control samples
Authors: Sharma Anubhav , Masters Karen L , Stark David V , Garland James , Drory Niv , Fraser-McKelvie Amelia , Weijmans Anne-Marie 
Published: September 2023

ABSTRACT
Gas-rich galaxies are typically star forming. We make use of H i-MaNGA, a programme of H i follow-up for the Mapping Nearby Galaxies at Apache Point Observatory (MaNGA) survey of the Sloan Digital Sky Surveys, to construct a sample of unusual neutral hydrogen (H i, 21 cm)-rich galaxies that have low star formation rates (SFRs), using infrared colour from the Wide-field Infrared Survey Explorer as a proxy for specific SFR. Out of a set of 1575 MaNGA galaxies with H i-MaNGA detections, we find that 83 (5 per cent) meet our selection criteria to be H i rich with low SFR. We construct two stellar mass-matched control samples: H i-rich galaxies with typical SFR (high SF control) and H i-poor galaxies with low SFR (low H i control). We investigate the properties of each of these samples, comparing physical parameters such as ionization state maps, stellar and ionized gas velocity and dispersion, environment measures, metallicity, and morphology to search for the reasons why these unusual H i-rich galaxies are not forming stars. We find evidence for recent external accretion of gas in some galaxies (via high counter-rotating fractions), along with some evidence for active galactic nucleus (AGN) feedback (from a high central low-ionization emission-line region and/or red geyser fraction), and bar quenching (via an enhanced strong bar fraction). Some galaxies in the sample are consistent with simply having their H i in a high angular momentum, large-radius, low-density disc. We conclude that no single physical process can explain all H i-rich, low-SFR galaxies.