bluuebunny/crossref_metadata_2025_split huggingface dataset:
Inhibition of neddylation causes early developmental arrestment in mouse embryo because of the zygotic genome activation failure
Authors: Yang Guangping , Hu Saifei , Chen Jianhua , Chen Liangliang , Miao Hui , Li Na , Luo Hui , He Yanni , Qian Yun , Miao Congxiu , Feng Ruizhi 
Published: June 2023

Abstract
Background
Maternal protein degradation in mammalian preimplantation embryos has yet to be fully understood. One major pathway is the ubiquitin-proteasome system. Recently, new forms of ubiquitination such as neddylation have been found to play important roles in a wide variety of biological processes, including reproduction. However, the molecular mechanism of neddylation in the early embryonic development of mammals is mostly unknown.
Methods
The zygotes were collected through in vitro fertilization and the expression of marker genes during embryonic development and zygotic genome activation (ZGA) was monitored after 24,48,72,96 hours of culture with MLN4924 (specific inhibition of neddylation) using real-time quantitative PCR. Single-cell RNA sequencing and quantitative PCR were applied to monitor and validate the changes in the downstream transcriptome. We utilized immunofluorescence and Western blotting to detect the expression and localization of proteins in mouse embryos.
Results
Blocking neddylation in mouse zygotes led to a statistically significant decrease in the cleavage rate to the 2-cell stage. Transcriptional profiling showed genes differentially expressed in pathways involving cell fate determination, cell differentiation, and cytoskeletal proteins. The expressions of zygotic ZGA markers were significantly reducejiand, indicating a significant downstream alteration in relevant pathways leading to the 2-cell stage arrest phenotype. A decrease in the level of RNA polymerase II in the nucleus was detected, showing impaired gene transcription in the embryo. We also identified a decrease in methyltransferase expression and concomitant reduction in histone H3K4 trimethylation, which may be the molecular mechanism of early embryonic developmental arrest caused by neddylation inhibition. Reduction of Yap1 was detected, suggesting aberrant downstream reactions of the Hippo signaling pathway. It also addressed the problem of the neddylation inhibition caused early embryonic arrest. Our study shed light upon new forms of ubiquitination regulating mammalian embryonic development and may contribute to further investigation of female infertility pathology.
Conclusions
Our data suggest that blocking neddylation leads to ZGA failure, possibly due to a decrease in H3K4me3 caused by a decrease in methyltransferase (KMT2D).
Diagnostic value of CA125, HE4, systemic immune‑inflammatory index (SII), fibrinogen-to-albumin ratio(FAR), and prognostic nutritional index(PNI) in the Preoperative Investigation of ovarian Masses
Authors: Song Liyun , Qi Jie , Zhao Jing , Bai Suning , Wu Qi , Xu Ren 
Published: June 2023

Abstract
Background
The aim of this study was to ascertain the diagnostic accuracy of Carbohydrate Antigen 125(CA125), Human Epididymis Protein 4(HE4), systemic immune-inflammation index (SII), fibrinogen-to-albumin ratio (FAR), prognostic nutritional index (PNI), and their combinations for ovarian cancer (OC) in order to discover an optimal combined diagnostic index for early diagnosis of OC. A thorough investigation was conducted to ascertain the correlation between these markers and the pathological characteristics of OC, thereby furnishing a foundation for the early identification and treatment of this disorder.
Methods
170 patients with documented OC and benign ovarian tumors (BOTs) treated at Hebei General Hospital between January 2019 and December 2022 were included in this retrospective study. The formula for serum inflammation related markers was: FAR = fibrinogen(g/L)/ albumin(g/L); PNI = albumin (g/L) + 5 × lymphocyte counts (109/L); SII = platelet count (109/L) × neutrophil count (109/L)/ lymphocyte count (109/L). Data analysis was conducted with IBM SPSS statistics version V26.0 software, MedCalc Statistical Software version 19.4.0 software, and R Environment for Statistical Computing software (R Foundation for Statistical Computing).
Results
The isolated CA125 tested showed the best application value to differentiate BOTs from OC when the defined variables were compared separately. The combination of CA125, HE4, FAR, SII, and PNI displayed a greater area under the ROC curve (AUC) than any one of them or other combinations of the five variables. Compared to CA125 alone, the combination of CA125, HE4, FAR, SII, and PNI showed a slight gain in sensitivity (83.91%), negative predictive value (NPV) (83.91%), accuracy (85.88%), and a decrease in negative likelihood ratio (LR) (0.180%). Higher preoperative CA125, HE4, SII, and FAR levels and lower PNI levels predicted a higher probability of advanced OC progression and lymph node metastasis. FAR had a better application value than other inflammation-related markers (PNI and SII).
Conclusions
The study suggested that preoperative serum SII, PNI, and FAR might potentially be clinically valuable markers in patients with OC. FAR had a better application value than other inflammation-related markers (PNI and SII). As we delve deeper into the inflammatory mechanisms associated with tumors, we may discover more effective combinations of tumor and inflammatory biomarkers.
Structure of Health Information With Different Information Models: Evaluation Study With Competency Questions
Authors: Rossander Anna , Karlsson Daniel 
Published: June 2023

Background
There is a flora of health care information models but no consensus on which to use. This leads to poor information sharing and duplicate modelling work. The amount and type of differences between models has, to our knowledge, not been evaluated.

Objective
This work aims to explore how information structured with various information models differ in practice. Our hypothesis is that differences between information models are overestimated. This work will also assess the usability of competency questions as a method for evaluation of information models within health care.

Methods
In this study, 4 information standards, 2 standards for secondary use, and 2 electronic health record systems were included as material. Competency questions were developed for a random selection of recommendations from a clinical guideline. The information needed to answer the competency questions was modelled according to each included information model, and the results were analyzed. Differences in structure and terminology were quantified for each combination of standards.

Results
In this study, 36 competency questions were developed and answered. In general, similarities between the included information models were larger than the differences. The demarcation between information model and terminology was overall similar; on average, 45% of the included structures were identical between models. Choices of terminology differed within and between models; on average, 11% was usable in interaction with each other. The information models included in this study were able to represent most information required for answering the competency questions.

Conclusions
Different but same same; in practice, different information models structure much information in a similar fashion. To increase interoperability within and between systems, it is more important to move toward structuring information with any information model rather than finding or developing a perfect information model. Competency questions are a feasible way of evaluating how information models perform in practice.
Variasi Konsentrasi Plant Growth Promoting Rhizobacteria (PGPR) Rendaman Akar Bambu Menghambat Pertumbuhan Jamur Fusarium oxysporum Secara In Vitro
Authors: I Kadek Sandiase  , Ni Luh Putu Manik Widiyanti  , I Wayan Sukra Warpala  
Published: June 2023

Salah satu agen hayati yang berpotensi sebagai biopestisida adalah plant growth promoting rhizobacteria (PGPR). PGPR memiliki kemampuan dalam menekan pertumbuhan jamur Fusarium oxysporum melalui mekanisme secara langsung dan secara tidak langsung. PGPR diketahui ada pada akar bambu yang memiliki kemampuan meningkatkan pertumbuhan tanaman bambu dan menekan aktivitas fitopatogen. Penelitian ini bertujuan untuk mengetahui daya hambat serta konsentrasi optimum dari PGPR rendaman akar bambu dalam menghambat pertumbuhan jamur Fusarium oxysporum. Metode pengujian digunakan adalah metode uji antagonis pada media PDA, dan media campuran PDA dan NA. Variasi konsentrasi PGPR rendaman akar bambu yang digunakan adalah 2,5, 5, 7,5 dan 10%. Data yang diperoleh dalam penelitian ini dianalisis secara deskriptif dan statistik. Analisis statistik menggunakan uji One Way Anova dan dilanjutkan dengan uji Tukey HSD pada taraf signifikansi 0,05. Hasil penelitian ini menunjukkan bahwa ada perbedaan daya hambat PGPR rendaman akar bambu berbagai konsentrasi terhadap pertumbuhan jamur Fusarium oxysporum pada media uji PDA dan media campuran. Konsentrasi PGPR rendaman akar bambu yang paling efektif dalam menghambat pertumbuhan jamur Fusarium oxysporum adalah 10%.
Guardianship, Its Importance and Developments: A Comparative Study Between Shariah Law and the Positive Law
Authors: Olayemi Abdul Azeez Maruf , Olagunju Anthoney Gbadebo 
Published: June 2023

Guardianship ‘Tutelae’ as a legal concept is as old as mankind. In antiquity, the communal and societal guardian is referred to as a messenger of God, prophet, priest and etc. He or she is believed to be a divine guardian that is appointed by God to lead each community or society, custodis mortalis. However, guardianship as an institution is not restricted to the communal and societal systems alone; it is also important to the family system and to the individual person when he or she is a minor or incapacitated. The institution is important to ensure that the interest of the present and future generations and the care of the people as o whole are preserved. However, the institution has positively undergone various developments in the present. Its importance and its transformations are discussed in this paper from the perspective of Shariah and Positive Laws.
Penerapan Teori Bentuk Estetik Dewitt H. Parker Sebagai Paradigma dalam Ranah Apresiasi Musik
Authors: Wulanda Gigih Alfajar Novra 
Published: June 2023

Perkembangan zaman yang ditandai dengan laju pesat teknologi turut mempengaruhi musik baik dari sisi produksi, distribusi bahkan sampai apresiasi. Realita bahwa begitu mudahnya orang “mengkonsumsi” musik tidak serta merta juga dengan sendirinya orang mengapresiasi karya musik yang dikonsumsinya. Dengan kata lain “laju konsumsi” belum tentu selaras dengan “laju apresiasi”. Metode penelitian kepustakaan yang meliputi literatur primer maupun sekunder sebagai sumber data digunakan untuk menemukan konsep-konsep ataupun teori yang akan diketengahkan dalam tulisan ini. Tulisan ini menawarkan pendekatan apresiasi berbasis paradigma estetika yang berupaya mensintesiskan dua disiplin yaitu apresiasi musik dan estetika. Dengan demikian pemahaman akan dasar-dasar apresiasi musik maupun teori-teori estetika menjadi prasyarat yang wajib dipenuhi. Pemahaman dasar apresiasi setidaknya meliputi bahan dasar musik seperti nada, elemen waktu, melodi, harmoni dan tonalitas, tekstur serta dinamika. Sedangkan perihal teori estetika, penulis menerapkan prinsip estetika DeWitt H. Parker yang meliputi organic unity, theme, thematic variation, balance, evolution dan hierarchy. Penerapan teori estetika tersebut sebagai sebuah paradigma dapat mempermudah sekaligus meningkatkan standar apresiasi karena membuatnya lebih sistematis dan terstruktur.
EL PRESUPUESTO E INDUSTRIA PARA LA DEFENSA ECUATORIANA, UNA MIRADA DEL ANTES Y DESPUÉS DE LA CONSTITUCIÓN DE MONTECRISTI.
Authors: GRANJA SÁNCHEZ MARCO ANTONIO , MANZANO TERÁN LUIS SANTIAGO 
Published: June 2023

El presente trabajo de investigación tiene como objetivo principal el analizar el presupuesto militar de las Fuerzas Armadas del Ecuador antes y después de la entrada en vigor de la Constitución de 2008 desde una perspectiva de la Economía de la Defensa, comparándolo con años anteriores y posicionándolo con otros países de la región en términos del porcentaje del PIB y en millones de dólares. Así mismo, se busca describir las acciones que durante este período han incidido para llegar a la situación actual de la industria militar ecuatoriana y cómo las decisiones políticas han impactado a lo largo de este período.
Las cifras fueron extraídas de la página del Banco Mundial que utiliza los datos del Instituto Internacional de Investigación para la paz de Estocolmo, o sus siglas en inglés SIPRI (Stockholm International Peace Research Institute). Estos valores se los comparó con los entregados por parte del Ministerio de Defensa Nacional a este equipo investigador que los ha dividido en gasto corriente e inversión. De esta manera se ha cuantificado la inversión en el mismo período haciendo énfasis en los años 2008 al 2020 donde se elabora un análisis de la calidad de la inversión en base a las compras realizadas en el mismo período.
Los resultados de esta investigación permitirán a los entes planificadores de nivel estratégico-político tomar decisiones que mejoren la calidad del gasto en defensa, donde se responda a una política de estado planificada, más no a una emergencia circunstancial de una sola fuerza o coyunturas políticas. Así mismo, permitirá evidenciar la importancia de desarrollar a la industria de la defensa, pues es un eslabón más del desarrollo de un país, que permitirá al Ecuador ser menos dependiente de la tecnología e insumos de otros países.
Significance and special features of legal communication: foreign experience
Authors: Fedyshyn O.M. , Bondarenko V.A. 
Published: June 2023

Legal communication plays a very important role in understanding law and legal discourse, promoting human interaction. Legal communication is defined as the process of exchanging or disseminating ideas, information, views or opinions, transmitted electronically or by means of gestures, speech or written speech, and it relates to legal aspects and is binding in nature. It is noted that it is necessary to understand the main features of legal communication in order to be able to participate effectively in it. The purpose of the article is to reveal the significance of legal communication and its special features in the legal sphere of interpersonal interaction, using foreign experience. Legal communication has proven to be a key aspect of the legal profession, as it covers a wide range of communication methods used in different legal contexts. It requires a high level of professionalism, attention to detail, objectivity, clarity and accuracy, timeliness, confidentiality, consistency, etc. Legal professionals need to know the ins and outs of legal communication to be effective in their work, from drafting legal documents to presenting arguments in court. By following these features, legal professionals can ensure that their communication is truly effective, appropriate and in the best interests of their clients. Legal communication is an important tool for achieving the desired results in court proceedings, ensuring compliance with the law, and maintaining the trust and respect of all parties involved. In general, clear and concise legal communication is essential for the proper functioning of the legal system, protection of the rights and interests of clients. Legal communication helps to establish and maintain the coherence of the legal system, optimize legal processes and procedures, ensure compliance with legal requirements, and promote honesty and fairness in the legal system. Legal communication also plays a crucial role in ensuring access to justice for everyone. Effective communication of legal concepts and procedures can help individuals to understand their rights and responsibilities, and to navigate the legal system with confidence. When legal concepts and requirements are communicated clearly and consistently, everyone is held to the same standards, regardless of their background or status.
Review of the monograph "Actual issues of the modern paradigm and prospects for the development of agrarian and land law of Ukraine"
Authors: Chabanenko M. 
Published: June 2023

Taking into account the set of reasons of an objective and subjective nature, today there is an urgent need to revise the ideas, views, concepts established in the theory of agrarian and land law on the legal nature of modern agrarian and land law and a gradual transition to a new paradigm of legal regulation of agrarian, land and related social relations taking place in the agricultural sector.
It is worth noting that in recent years there have been high-profile events in society and the state in the field of globalization of agri-food markets, liberalization of trade, modernization of management of the agri-food sector, implementation of land rights, use and protection of land, and in general state regulation of agrarian and land relations. The above allows us to ascertain the presence of systemic problems in modern agrarian and land legislation, since the established principles, methods and means of legal regulation do not fully ensure the effective functioning of the specified relations. In addition, both agrarian and land law belong to branch legal sciences that are dynamically developing. Therefore, in modern conditions, the doctrine of agrarian and land law faces new problems, caused primarily by global, national and regional challenges of an economic, social, political, ecological and legal nature.
In view of the above, the collective monograph "Agrarian and Land Law of Ukraine: Modern Paradigm and Development Prospects" edited by Doctor of Law, Professor Anatoliy Pavlovich Hetman and Doctor of Law, Professor Tetyana Viktorivna Kurman is extremely relevant and timely. The relevance of this work lies in the need for a scientific and theoretical study of the methodological and scientific foundations of the legal regulation of agrarian and land relations and the improvement of the mechanism of legal regulation of these relations.
Certain aspects of guaranteeing the implementation of the rights of internally displaced children in Ukraine
Authors: Menzhul M.V. 
Published: June 2023

The article examines certain aspects of guaranteeing the implementation of the rights of internally displaced children in Ukraine. It was noted that one of the most vulnerable groups in the conditions of war are children, in particular, not only IDP children, refugee children, children staying in occupied territories, in the territories of active hostilities, but also children living in relatively safe territories.
The importance of guaranteeing the safety of children in the conditions of war in Ukraine is noted, as well as taking into account the best interests of the child to provide the child with such protection and care as is necessary for his well-being, taking into account the rights and obligations of his parents or other legal representatives. It is justified that it is the state that has the obligation to ensure that the institutions, services and bodies responsible for the care of children or their protection comply with the norms established by the competent authorities, in particular, in the field of safety and security.
The implementation of the principle of the best interests of the child in the legislation and judicial practice of Ukraine was analyzed, and the practice during the war was clarified. It is substantiated that in the conditions of war, children need proper protection, social support, guarantee of quality legal assistance, implementation of the right to housing, education, family reunification, etc. Martial law is not a reason to limit the application of the principle of the best interests of the child.
It was reasonable, from the point of view of protecting the rights of the child and guaranteeing its best interests, to give children from the age of 14 the opportunity to independently apply for registration as IDPs. It has been proven that in Ukraine, in order to guarantee the realization of the rights of IDP children, various instruments are implemented, which are based on the principle of the best interests of the child and aim to guarantee the safety and respect for the child's dignity.
Peculiarities of the appointment of employees of the state executive service
Authors: Manzhak B.S. 
Published: June 2023

The article examines the peculiarities of the appointment of employees of the state executive service bodies, who are empowered with state-authority and who are authorized to represent the interests of the state in the field of executive proceedings. Since the full-scale Russian invasion and the introduction of martial law fundamentally changed the situation in the country, particularly in the system of enforcement of court decisions. At the most difficult moment, ancient systemic problems fully manifested themselves. The problem of the quality and professional competence of civil service personnel, including employees of state executive service bodies, has become acute.
Having analyzed the normative legal acts in the researched area, it was concluded that the Laws of Ukraine "On Enforcement Proceedings" and "On Bodies and Persons Carrying Out Enforcement of Court Decisions and Decisions of Other Bodies" adopted in 2016 have lost the system of norms characteristic of the previous ones normative legal acts that regulated the activities of state executive service bodies. In the current laws, the norms regulating the activities of private executors have superseded the norms regulating the activities of state executors in Ukraine to other laws and subordinate legal acts. Therefore, supporting the positions of other researchers of this issue, the activities of employees of the state executive service should receive decent legal regulation at the level of law, and taking into account the accumulation of a critical mass of legal norms regulating executive proceedings at the level of codified law – the Executive Code. Also, taking into account the appointment of persons to positions in the civil service, including bodies of the state executive service, during martial law without competitive selection, tests should be established for all persons appointed to positions in the civil service. Therefore, the provisions of Art. 35 of the Law of Ukraine "On Civil Service" should apply to all civil servants.