bluuebunny/crossref_metadata_2025_split huggingface dataset:
A high-precision bidirectional time-transfer system over a single fiber based on wavelength-division multiplexing and time-division multiplexing
Authors: Guo Xinxing , Liu Bo , Kong Weicheng , Quan Honglei , Zhang Yucan , Liu Tao , Dong Ruifang , Zhang Shougang 
Published: January 2023

In this paper, a high-precision bidirectional time-transfer system over a single fiber based on wavelength-division multiplexing and time-division multiplexing (SFWDM-TDM) is proposed, which combines the advantages of wavelength-division multiplexing and time-division multiplexing. It uses two dense wavelength-division channels to effectively suppress the problem of optical fiber reflection. At the same time, the time-division multiplexing method is used in combination with sampling and holding the time to complete the multi-user task. In hardware, we optimized the carrier processing and the high-precision time-delay control module of the SFWDM-TDM system to complete high-precision time-transfer equipment. In software and algorithm, the optical fiber time-interval measurement method and measurement times are optimized, and the SFWDM-TDM system reaches a synchronization accuracy of 8.9 ps at 1 s. Finally, a real-time detection mechanism with self-recovery ability is added to the system. This lays the foundation for a reliable, long-distance, high-precision, and multi-user mode optical fiber time- and frequency-transfer network.
Spatiotemporal shaping of high power laser pulses based on stimulated brillouin scattering
Authors: Zhu Xuehua , Liu Chuangchuang , Gao Qilin , Su Hao , Sun Sizhou 
Published: January 2023

We proposed and numerically demonstrated the combination of temporal and spatial shaping of high power nanosecond laser pulses based on stimulated Brillouin scattering (SBS) in this paper. With the intensity-dependence characteristic of SBS, the higher intensity parts of the laser beam obtain higher reflectivity, and the incident non-uniform high power laser beam would be well smoothed. A parameter adjustable feedback control loop was used to tailor the output temporal profile by pre-compensating the temporal profile of the input pump. In our numerical simulation, a 3 ns super-Gaussian shaped single-frequency laser pulse with a 527 nm wavelength was used as the pump. And the heavy fluorocarbon FC-70 was chosen as the Brillouin medium. Simulation results show that the laser spatial modulation can be significantly pulled down when the energy efficiency is maintained above 90% in our beam smoothing system with suitable laser intensity. The flat-toped laser pulses both in temporal and spatial domain were demonstrated to be achievable simultaneously. The method proposed here paves a simple and effective way to optimizing the near field pattern and temporal shape of high power laser systems.
Aortic valve morphology and paravalvular leak regression after a self-expandable transcatheter aortic valve replacement
Authors: Jin Qinchun , Li Wei , Chen Shasha , Li Mingfei , Zhou Daxin , Zhang Xiaochun , Ge Junbo 
Published: January 2023

Aims: The study aimed to compare paravalvular leak (PVL) changes after a transcatheter aortic valve replacement (TAVR) with self-expandable prosthesis between different aortic valve morphologies and evaluate the impact of paravalvular leak regression on clinical prognosis.Methods: Patients with aortic stenosis (AS) successfully treated with a self-expandable TAVR who were followed up for at least 1 year at our centre were consecutively enrolled from January 2016 to August 2019. Paired serial changes in paravalvular leak and other haemodynamic parameters by echocardiography were collected and compared between the bicuspid valve (BAV) and tricuspid aortic valve (TAV). A logistic regression model was used to explore the predictors of paravalvular leak regression (&lt;1 grade) 1 year after transcatheter aortic valve replacement, while its impact on subsequent clinical outcomes (all-cause mortality and rehospitalisation for heart failure (HF)) was further evaluated using Kaplan–Meier analysis.Results: A total of 153 bicuspid valve and 114 tricuspid aortic valve patients were finally enrolled; haemodynamic parameters and paravalvular leak severity were comparable before the discharge between the two groups. The peak transaortic velocity, mean transvalvular gradient, and effective orifice area all significantly improved (p &lt; 0.05) without intergroup differences at all follow-up timepoints. Significant paravalvular leak reduction was observed only in the TAV group (1.75% vs. 4.39%, p = 0.029), while moderate paravalular leak was still more prevalent in BAV (7.19% vs. 1.75%, p = 0.041) at the 1-year follow-up. Multivariable analyses identified the bicuspid valve, asymmetric calcification, and undersizing as independent predictors of failure of the 1-year paravalvular leak reduction in patients with mild or moderate paravalvular leak after discharge. Patients without a paravalvular leak reduction within 1 year showed a relatively higher 2-year all-cause mortality and HF (HR: 5.994, 95% CI: 1.691–21.240, and p = 0.053) rates thereafter.Conclusion: In AS patients after self-expandable transcatheter aortic valve replacement, paravalvular leak regression within 1 year was less prevalent in bicuspid valve morphology. The failure of paravalvular leak reduction might lead to an increased risk of poorer prognosis in the long run.
Multiple aspects of lymphatic dysfunction in an ApoE−/− mouse model of hypercholesterolemia
Authors: Davis Michael J. , Scallan Joshua P. , Castorena-Gonzalez Jorge A. , Kim Hae Jin , Ying Lim Hwee , Pin Yeo Kim , Angeli Veronique 
Published: January 2023

Introduction: Rodent models of cardiovascular disease have uncovered various types of lymphatic vessel dysfunction that occur in association with atherosclerosis, type II diabetes and obesity. Previously, we presented in vivo evidence for impaired lymphatic drainage in apolipoprotein E null (ApoE−/−) mice fed a high fat diet (HFD). Whether this impairment relates to the dysfunction of collecting lymphatics remains an open question. The ApoE−/− mouse is a well-established model of cardiovascular disease, in which a diet rich in fat and cholesterol on an ApoE deficient background accelerates the development of hypercholesteremia, atherosclerotic plaques and inflammation of the skin and other tissues. Here, we investigated various aspects of lymphatic function using ex vivo tests of collecting lymphatic vessels from ApoE+/+ or ApoE−/− mice fed a HFD.Methods: Popliteal collectors were excised from either strain and studied under defined conditions in which we could quantify changes in lymphatic contractile strength, lymph pump output, secondary valve function, and collecting vessel permeability.Results: Our results show that all these aspects of lymphatic vessel function are altered in deleterious ways in this model of hypercholesterolemia.Discussion: These findings extend previous in vivo observations suggesting significant dysfunction of lymphatic endothelial cells and smooth muscle cells from collecting vessels in association with a HFD on an ApoE-deficient background. An implication of our study is that collecting vessel dysfunction in this context may negatively impact the removal of cholesterol by the lymphatic system from the skin and the arterial wall and thereby exacerbate the progression and/or severity of atherosclerosis and associated inflammation.
Association mapping for broomrape resistance in sunflower
Authors: Calderón-González Álvaro , Pérez-Vich Begoña , Pouilly Nicolas , Boniface Marie-Claude , Louarn Johann , Velasco Leonardo , Muños Stéphane 
Published: January 2023

IntroductionSunflower breeding for resistance to the parasitic plant sunflower broomrape (Orobanche cumana Wallr.) requires the identification of novel resistance genes. In this research, we conducted a genome-wide association study (GWAS) to identify QTLs associated with broomrape resistance.MethodsThe marker-trait associations were examined across a germplasm set composed of 104 sunflower accessions. They were genotyped with a 600k AXIOM® genome-wide array and evaluated for resistance to three populations of the parasite with varying levels of virulence (races EFR, FGV, and GTK) in two environments.Results and DiscussionThe analysis of the genetic structure of the germplasm set revealed the presence of two main groups. The application of optimized treatments based on the general linear model (GLM) and the mixed linear model (MLM) allowed the detection of 14 SNP markers significantly associated with broomrape resistance. The highest number of marker-trait associations were identified on chromosome 3, clustered in two different genomic regions of this chromosome. Other associations were identified on chromosomes 5, 10, 13, and 16. Candidate genes for the main genomic regions associated with broomrape resistance were studied and discussed. Particularly, two significant SNPs on chromosome 3 associated with races EFR and FGV were found at two tightly linked SWEET sugar transporter genes. The results of this study have confirmed the role of some QTL on resistance to sunflower broomrape and have revealed new ones that may play an important role in the development of durable resistance to this parasitic weed in sunflower.
A metabolomic platform to identify and quantify polyphenols in coffee and related species using liquid chromatography mass spectrometry
Authors: Castro-Moretti Fernanda R. , Cocuron Jean-Christophe , Castillo-Gonzalez Humberto , Escudero-Leyva Efrain , Chaverri Priscila , Guerreiro-Filho Oliveiro , Slot Jason C. , Alonso Ana Paula 
Published: January 2023

IntroductionProducts of plant secondary metabolism, such as phenolic compounds, flavonoids, alkaloids, and hormones, play an important role in plant growth, development, stress resistance. The plant family Rubiaceae is extremely diverse and abundant in Central America and contains several economically important genera, e.g. Coffea and other medicinal plants. These are known for the production of bioactive polyphenols (e.g. caffeine and quinine), which have had major impacts on human society. The overall goal of this study was to develop a high-throughput workflow to identify and quantify plant polyphenols.MethodsFirst, a method was optimized to extract over 40 families of phytochemicals. Then, a high-throughput metabolomic platform has been developed to identify and quantify 184 polyphenols in 15 min.ResultsThe current metabolomics study of secondary metabolites was conducted on leaves from one commercial coffee variety and two wild species that also belong to the Rubiaceae family. Global profiling was performed using liquid chromatography high-resolution time-of-flight mass spectrometry. Features whose abundance was significantly different between coffee species were discriminated using statistical analysis and annotated using spectral databases. The identified features were validated by commercially available standards using our newly developed liquid chromatography tandem mass spectrometry method.DiscussionCaffeine, trigonelline and theobromine were highly abundant in coffee leaves, as expected. Interestingly, wild Rubiaceae leaves had a higher diversity of phytochemicals in comparison to commercial coffee: defense-related molecules, such as phenylpropanoids (e.g., cinnamic acid), the terpenoid gibberellic acid, and the monolignol sinapaldehyde were found more abundantly in wild Rubiaceae leaves.
MePAL6 regulates lignin accumulation to shape cassava resistance against two-spotted spider mite
Authors: Yao Xiaowen , Liang Xiao , Chen Qing , Liu Ying , Wu Chunling , Wu Mufeng , Shui Jun , Qiao Yang , Zhang Yao , Geng Yue 
Published: January 2023

IntroductionThe two-spotted spider mite (TSSM) is a devastating pest of cassava production in China. Lignin is considered as an important defensive barrier against pests and diseases, several genes participate in lignin biosynthesis, however, how these genes modulate lignin accumulation in cassava and shape TSSM-resistance is largely unknown.MethodsTo fill this knowledge gap, while under TSSM infestation, the cassava lignin biosynthesis related genes were subjected to expression pattern analysis followed by family identification, and genes with significant induction were used for further function exploration.ResultsMost genes involved in lignin biosynthesis were up-regulated when the mite-resistant cassava cultivars were infested by TSSM, noticeably, the MePAL gene presented the most vigorous induction among these genes. Therefore, we paid more attention to dissect the function of MePAL gene during cassava-TSSM interaction. Gene family identification showed that there are 6 MePAL members identified in cassava genome, further phylogenetic analysis, gene duplication, cis-elements and conserved motif prediction speculated that these genes may probably contribute to biotic stress responses in cassava. The transcription profile of the 6 MePAL genes in TSSM-resistant cassava cultivar SC9 indicated a universal up-regulation pattern. To further elucidate the potential correlation between MePAL expression and TSSM-resistance, the most strongly induced gene MePAL6 were silenced using virus-induced gene silencing (VIGS) assay, we found that silencing of MePAL6 in SC9 not only simultaneously suppressed the expression of other lignin biosynthesis genes such as 4-coumarate--CoA ligase (4CL), hydroxycinnamoyltransferase (HCT) and cinnamoyl-CoA reductase (CCR), but also resulted in decrease of lignin content. Ultimately, the suppression of MePAL6 in SC9 can lead to significant deterioration of TSSM-resistance.DiscussionThis study accurately identified MePAL6 as critical genes in conferring cassava resistance to TSSM, which could be considered as promising marker gene for evaluating cassava resistance to insect pest.
Slope position- mediated soil environmental filtering drives plant community assembly processes in hilly shrublands of Guilin, China
Authors: Chen Kunquan , Pan Yuanfang , Li Yeqi , Cheng Jiaying , Lin Haili , Zhuo Wenhua , He Yan , Fang Yaocheng , Jiang Yong 
Published: January 2023

Background and aimsA major goal of community ecology focuses on trying to understand how environmental filter on plant functional traits drive plant community assembly. However, slopes positions- mediated soil environmental factors on community-weighted mean (CWM) plant traits in shrub community has not been extensively explored to analyze and distinguish assembly processes.MethodsHere, we surveyed woody shrub plant communities from three slope positions (foot, middle, and upper) in a low hilly area of Guilin, China to assess differences in functional trait CWMs and environmental factors across these positions. We also measured the CWMs of four plant functional traits including specific leaf area, leaf dry matter content, leaf chlorophyll content, and leaf thickness and nine abiotic environmental factors, including soil water content, soil organic content, soil pH, soil total nitrogen, soil total phosphorus, soil total potassium, soil available nitrogen, soil available phosphorus, and soil available potassium. We used ANOVA and Tukey HSD multiple comparisons to assess differences in functional trait CWMs and environmental factors across the three slope positions. We used redundancy analysis (RDA) to compare the relationships between CWMs trait and environmental factors along three slope positions, and also quantified slope position-mediated soil environmental filtering on these traits with a three-step trait-based null model approach.ResultsThe CWMs of three leaf functional traits and all soil environmental factors except soil pH showed significant differences across the three slope positions. Soil total nitrogen, available nitrogen, available potassium, and soil organic matter were positively correlated with the CWM specific leaf area and leaf chlorophyll content along the first RDA axis and soil total potassium, total phosphorous, and soil water content were positively correlated with the CWM leaf dry matter content along the second RDA axis. Environmental filtering was detected for the CWM specific leaf area, leaf dry matter content, and leaf chlorophyll content but not leaf thickness at all three slope positions.ConclusionsUltimately, we found that soil environmental factors vary along slope positions and can cause variability in plant functional traits in shrub communities. Deciduous shrub species with high specific leaf area, low leaf dry matter content, and moderate leaf chlorophyll content dominated at the middle slope position, whereas evergreen species with low specific leaf area and high leaf dry matter content dominated in slope positions with infertile soils, steeper slopes, and more extreme soil water contents. Altogether, our null model approach allowed us to detect patterns of environmental filtering, which differed between traits and can be applied in the future to understand community assembly changes in Chinese hilly forest ecosystems.
Nicotiana tabacum as a dead-end trap for adult Diaphorina citri: A potential biological tactic for protecting citrus orchards
Authors: Zheng Lixia , Xu Qianqian , Gong Gu , Liao Yonglin , Yu Min , Shabala Sergey , Chen Wensheng , Wu Weijian 
Published: January 2023

The Asian citrus psyllid, Diaphorina citri Kuwayama (Hemiptera: Liviidae), is a key vector of the causal agents of Huanglongbing (HLB), a devastating disease affecting citrus almost worldwide. Nicotiana tabacum L. is an important commercial crop in China. Field observations suggested that D. citri adults die on N. tabacum leaves when grown nearby citrus orchards. In this study, the preference for and survivorship of D. citri adults on N. tabacum and their feeding behavior were investigated. The results showed that D. citri adults were attracted to N. tabacum and to the green leaf volatiles (GLVs) (Z)-3-hexenol and (E)-2-hexenol. The survival of D. citri adults on N. tabacum was less than 30 h, which was shorter than that for adults without food (35 h) and on a suitable host Murraya exotica L. (29 days). Electrical penetration graph (EPG) recordings revealed that the pathway phase of D. citri on N. tabacum leaves consisted of four waveforms—the non-probing phase (NP), the pathway phase (PP, including intercellular probing of activity in the phloem (C) and phloem penetration (D)), phloem salivation (E1), and phloem ingestion (E2). Diaphorina citri only secreted saliva and ingested sap from phloem on N. tabacum leaves and spent the longest duration in phloem sap ingestion (E2). Moreover, L-nicotine, an important defense compound against insects in N. tabacum plants, was highly toxic to D. citri. These results suggested that N. tabacum plants could help to sustainably control the spread of D. citri and HLB when growing in and around citrus orchards.
Effect of Glomus intraradices on root morphology, biomass production and phosphorous use efficiency of Chinese fir seedlings under low phosphorus stress
Authors: Tian Yunlong , Xu Jingjing , Lian Xiaoqian , Wei Bo , Ma Xiangqing , Wu Pengfei 
Published: January 2023

IntroductionAvailable phosphorus (P) scarcity in the highly weathered soils of the subtropical forests in southern China is a serious concern. To ensure whether inoculation of arbuscular mycorrhizal fungi (AMF) with Chinese fir (Cunninghamia lanceolata) under low P stress conditions could promote its growth and P utilization capacity, an indoor pot simulation experiment was carried out with the different P supply treatments and Chinese fir seedlings as the tested material.MethodsThe experiment had two P supply treatments, no P supply (P0, 0 mmol·L-1 KH2PO4) and normal P supply (P1, 1.0 mmol·L-1 KH2PO4). The seedling in each P supply treatment was inoculated with Glomus intraradices (Gi), a widespread species of AMF in the natural environment, and with no AMF inoculation as a control treatment (CK). The Gi infection rate in the root system, root cortex tissue dissolution rate, root morphological indexes and biomass, whole plant P use efficiency, and root P use efficiency of Chinese fir were determined under different treatment conditions.Results and DiscussionThe results showed that P0 treatment significantly increased the Gi infection rate (p&lt; 0.05). After inoculating AMF with different P supply treatments, the root cortex tissue dissolution rate was considerably enhanced. In contrast, the Chinese fir’s root length and surface area were reduced; however, the root volume did not change significantly. The average root diameter in the P0 treatment and inoculated with AMF was significantly more prominent than in the uninoculated treatment (p&lt; 0.05). The root biomass and root-to-shoot ratio at different P supply treatments were significantly higher in the Gi infection treatment than in the CK group. Under different P supply treatments, root inoculation with Gi promoted root P use efficiency and whole plant P use efficiency. In conclusion, low P stress condition promoted the colonization of AMF in the root system, increased the dissolution of root cortex tissue, root volume, and the average diameter, and promoted root biomass accumulation and P use efficiency.
In a split second: Handwriting pauses in typical and struggling writers
Authors: Pascual Mariona , Soler Olga , Salas Naymé 
Published: January 2023

IntroductionA two-second threshold has been typically used when analyzing the writing processes. However, there is only a weak empirical basis to claim that specific average numbers and durations of pauses may be associated with specific writing processes. We focused on handwriting execution pauses, because immature writers are known to struggle with transcription skills. We aimed to provide an evidence-based account of the average number and duration of handwriting pauses in the mid-Primary grades and to identify process-level markers of writing difficulties.MethodsEighty 3rd and 5th graders, with and without writing difficulties, participated in the study. We examined pauses in a handwriting-only task, to be able to isolate those which could only be attributed to handwriting processes. Letter features were considered, as well as children’s handwriting fluency level.ResultsThe average duration of handwriting pauses was around 400ms, in line with assumptions that transcription pauses would fall under the 2,000ms threshold. We found that 3rd graders made more and longer pauses than 5th graders. Struggling writers made a similar number of pauses across grades than typically-developing children, although they were significantly longer, even after controlling for the effect of handwriting fluency.DiscussionOur findings provide an evidence-based account of the duration of handwriting pauses. They also suggest that children need fewer and shorter handwriting pauses as they progress in automatizing transcription. However, some young writers struggle with letter formation even after 3 to 5 years of instruction.