bluuebunny/crossref_metadata_2025_split huggingface dataset:
Activation of Inflammasome complex in nasopharyngeal epithelial cells from patients with Coronavirus disease 2019 contributes to inflammatory state and worse disease outcomes
Authors: Bagheri‐Hosseinabadi Zahra , Dehghan‐Banadaki Mohsen , Sharifi Gholamhossein Taghipour Khaje , Abbasifard Mitra 
Published: May 2023

AbstractPathogenesis of Coronavirus disease 2019 (COVID‐19) has been associated with dysregulation of both adaptive and innate immune systems. Hence, we determined the contribution of inflammasome in the nasopharyngeal epithelial cells isolated from COVID‐19 subjects to disease pathogenesis and outcomes. Epithelial cells from 150 COVID‐19 patients and 150 healthy controls were yielded through nasopharyngeal swab sampling. Patients were categorized into three groups of those with clinical presentations/need hospitalization, with clinical presentations/no need hospitalization and cases without clinical symptoms/no need hospitalization. Finally, the transcriptional amount of inflammasome related genes were assessed in the nasopharyngeal epithelial cells using qPCR. There was a significant upregulation of nod‐like receptor (NLR) family pyrin domain containing 1 (NLRP1), nod‐like receptor (NLR) family pyrin domain containing 3 (NLRP3), Apoptosis‐associated speck‐like protein containing a CARD (ASC) and Caspase‐1 mRNA expressions in patients compared to controls. NLRP1, NLRP3, ASC and Caspase‐1 were upregulated in epithelial cells of patients with clinical symptoms/need hospitalization and cases with clinical symptoms/no need hospitalization when compared to controls. There was a correlation between expression of inflammasome‐related genes and clinicopathological features. Abnormal expression of inflammasome‐related genes in the nasopharyngeal epithelial cells obtained from COVID‐19 patients may be of prognostic value to determine the intensity of the disease's outcomes and requirement for alternative supports in hospitals.
Early psychosocial risk factors and postnatal parental reflective functioning
Authors: Arkle Philippa , Larkin Fionnuala , Wang Ying , Lee Yujin , Fernandez Amy , Li Lydia Y. , Meins Elizabeth 
Published: May 2023

AbstractPsychosocial factors have been found to relate to parental reflective functioning (PRF), a parent's ability to mentalize about themselves and their child. Relations between maternal psychosocial risk factors and PRF were investigated in a community sample. A sample of mothers (n = 146) was assessed for risk factors when infants were 6 months, infant temperament was assessed using an observational measure, and PRF was assessed with the Parent Development Interview‐Revised (PDI). PRF was measured again with the Parental Reflective Functioning Questionnaire (PRFQ) when children were 4 years (n = 105) and 5 years (n = 92), with an additional sample of mothers (n = 48) tested at these two timepoints. Results showed that in infancy, total maternal psychosocial risk related to lower PDI‐PRF; regression analyses highlighted low socioeconomic status, unplanned pregnancy, and low maternal anxiety as independent predictors of lower PDI‐PRF. PDI‐PRF scores at 6 months did not relate to PRFQ scores, but PRFQ subscales showed stability over time from age 4–5. Results are discussed with regard to the impact of maternal psychosocial risk and infant temperament on PRF and the stability and concordance of PRF measures.
Recovery college dropout: A qualitative study of external, relational and course‐related dropout drivers in co‐produced mental health care
Authors: Andersen Ditte , Waldemar Anna Kristine , Kirkegaard Sine 
Published: May 2023

AbstractRecovery colleges (RCs) are rapidly spreading across Western countries, and research indicates beneficial outcomes of this co‐produced model of mental health care. Meanwhile, risks of adverse outcomes and RC dropout remain understudied. To address this research gap, we conducted qualitative interviews with 14 participants who dropped out of RC courses in Denmark. This article, adhering to the consolidated criteria for reporting qualitative research (COREQ), presents a typology of the main dropout drivers identified in our sample: external, relational and course‐related. External drivers involve practical obstacles, for example some participants feared taking public transportation and lacked access to alternative means of travelling to the courses. Relational drivers entail distressing interactions with educators or peer students, for example some participants felt stigmatized or intimidated. Course‐related drivers concern the content of the courses, for example some students considered the academic level too basic as their design did not take prior learning into account, while others experienced a sense of alienation because they were unable or unwilling to share the kind of personal experiences course assignments envisaged. In the discussion of our findings, we consider how different types of drivers call for different modes of responses. We discuss dilemmas related to the proposed responses for reducing or accepting RC dropout.
Predictors and social determinants ofHIVtreatment engagement among post‐partum Black women living withHIVin southeastern United States
Authors: Ojukwu Emmanuela N. , Cianelli Rosina , Rodriguez Natalia Villegas , Gattamorta Karina , De Oliveira Giovanna , Duthely Lunthita 
Published: May 2023

AbstractAimGuided by Mcleroy's socio‐ecological model, this study explored the predictors and social determinants of HIV treatment engagement among Black post‐partum women living with HIV.MethodQuantitative, research methodology.DesignWe conducted a retrospective, secondary data analysis of 143 Black post‐partum women living with HIV who received peripartum care in South‐Florida, United States, from 2009 to 2017. We examined odds of immediate post‐partum engagement at 3 months post‐partum, and ongoing primary care engagement at 12 months post‐partum.ResultsThe independent group analyses showed low levels of immediate post‐partum (32.9%) and ongoing primary care engagement (24.5%). At the intrapersonal level, maternal prenatal health significantly affected both immediate post‐partum and ongoing primary care engagement; and at the interpersonal level, HIV disclosure and intimate partner violence/abuse significantly affected immediate post‐partum engagement. Also, immediate post‐partum disengagement was a significant predictor for ongoing primary care disengagement.ConclusionThis study provides timely and critical information to address recent calls for awareness and interventions to address issues on health disparities and inequities among racialized communities.ImpactThe study provides significant evidence on the effects of social determinants of health on health outcomes for Black women living with HIV. Critical understanding and assessment of these factors, together with proper, proactive interventions may help to gauge such negative effects. Healthcare providers taking care of Black women living with HIV ought to be cognizant of these factors, assess at‐risk women and intervene accordingly to ensure that their care is not marginalized.Patient/Public ContributionThis study includes direct patient data from Black post‐partum women living with HIV who were seen at prenatal and post‐partum clinics wherein data for this study were obtained. The study results were presented locally, nationally and internationally to communities, organizations of healthcare providers, stakeholders and service‐users, who further corroborated our findings, and provided insights and future recommendations.
Nursing informatics competence profiles and perceptions of health information system usefulness among registered nurses: A latent profile analysis
Authors: Kaihlanen Anu‐Marja , Elovainio Marko , Virtanen Lotta , Kinnunen Ulla‐Mari , Vehko Tuulikki , Saranto Kaija , Heponiemi Tarja 
Published: May 2023

AbstractAimsTo identify different nursing informatics competence (NIC) profiles in nurses, examine the factors associated with profile memberships and examine the associations of the derived profiles with the nurses' perception of the usefulness of a health information system (HIS).DesignA cross‐sectional study.MethodsA sample of 3610 registered nurses responded to a nationwide survey in March 2020. A latent profile analysis was performed to identify NIC profiles based on three competence areas: nursing documentation, working in digital environment, and ethics and data protection. A multinomial logistic regression was carried out to examine the associations of demographic and background variables with the profile membership. Linear regression analyses were carried out to examine the association between the profile membership and perceived HIS usefulness.ResultsThree NIC profiles were identified and labelled as low, moderate and high competence groups. A younger age, recent graduation year, sufficient orientation and high‐rated proficiency as an HIS user were associated with nurses belonging to a high or moderate competence group relative to a low competence group. Competence group membership was associated with perceived HIS usefulness. The high competence group consistently expressed the highest usefulness of the HIS and the low competence group the lowest.ConclusionTailored training and support should be provided for nurses with different levels of informatics competence, thereby facilitating their ability to respond to increasingly digitalized work. This could contribute to higher usefulness of the HIS in terms of supporting the nurses' work tasks and promoting the quality of care.ImpactThis was the first study exploring latent profiles of informatics competence in nurses. Insights from this study are useful for nursing management to identify different competence profiles of their employees, provide support and training to meet their needs, and promote the successful use of an HIS.
SNHG1, a KLF4‐upregulated gene, promotes glioma cell survival and tumorigenesis under endoplasmic reticulum stress by upregulating BIRC3 expression
Authors: Zhang Hongqiang , Ma Binbin , Li Na , Zhang Li , Xu Jialu , Zhang Shuqi , Guo Ziming , Han Chuanchun , Xu Shasha , Li Xiaodong , Zhang Bo 
Published: May 2023

AbstractIncreasing evidence indicates that long noncoding RNAs (lncRNAs) play crucial roles in the resistance to endoplasmic reticulum (ER) stress in many cancers. However, ER stress‐regulated lncRNAs are still unknown in glioma. In the present study, we investigated the altered lncRNAs upon ER stress in glioma and found that small nucleolar RNA host gene 1 (SNHG1) was markedly increased in response to ER stress. Increased SNHG1 suppressed ER stress‐induced apoptosis and promoted tumorigenesis in vitro and in vivo. Further mechanistic studies indicated that SNHG1 elevated BIRC3 mRNA stability and enhanced BIRC3 expression. We also found that KLF4 transcriptionally upregulated SNHG1 expression and contributed to the ER stress‐induced SNHG1 increase. Collectively, the present findings indicated that SNHG1 is a KLF4‐regulated lncRNA that suppresses ER stress‐induced apoptosis and facilitates gliomagenesis by elevating BIRC3 expression.
Differential expression profiles of plasma exosomal microRNAs in dilated cardiomyopathy with chronic heart failure
Authors: Zhang Li , Zhang Ge , Lu Yongzheng , Gao Jiamin , Qin Zhen , Xu Shuai , Wang Zeyu , Xu Yanyan , Yang Yu , Zhang Jinying , Tang Junnan 
Published: May 2023

AbstractAs one of the most prevalent heritable cardiovascular diseases, dilated cardiomyopathy (DCM) induces cardiac insufficiency and dysfunction. Although genetic mutation has been identified one of the causes of DCM, the usage of genetic biomarkers such as RNAs for DCM early diagnosis is still being overlooked. In addition, the alternation of RNAs could reflect the progression of the diseases, as an indicator for the prognosis of patients. Therefore, it is beneficial to develop genetic based diagnostic tool for DCM. RNAs are often unstable within circulatory system, leading to the infeasibility for clinical application. Recently discovered exosomal miRNAs have the stability that is then need for diagnostic purpose. Hence, fully understanding of the exosomal miRNA within DCM patients is vital for clinical translation. In this study, we employed the next generation sequencing based on the plasma exosomal miRNAs to comprehensively characterize the miRNAs expression in plasma exosomes from DCM patients exhibiting chronic heart failure (CHF) compared to healthy individuals. A complex landscape of differential miRNAs and target genes in DCM with CHF patients were identified. More importantly, we discovered that 92 differentially expressed miRNAs in DCM patients undergoing CHF were correlated with several enriched pathways, including oxytocin signalling pathway, circadian entrainment, hippo signalling pathway‐multiple species, ras signalling pathway and morphine addiction. This study reveals the miRNA expression profiles in plasma exosomes in DCM patients with CHF, and further reveal their potential roles in the pathogenesis of it, presenting a new direction for clinical diagnosis and management of DCM patients with CHF.
Comparison between p‐distance and single‐locus species delimitation models for delineating reproductively tested strains of pennate diatoms (Bacillariophyceae) usingcox1,rbcLandITS
Authors: Franco Andréa de O. da R. , Ashworth Matt P. , Odebrecht Clarisse 
Published: May 2023

AbstractSeveral automated molecular methods have emerged for distinguishing eukaryote species based on DNA sequence data. However, there are knowledge gaps around which of these single‐locus methods is more accurate for the identification of microalgal species, such as the highly diverse and ecologically relevant diatoms. We applied genetic divergence, Automatic Barcode Gap Discovery for primary species delimitation (ABGD), Assemble Species by Automatic Partitioning (ASAP), Statistical Parsimony Network Analysis (SPNA), Generalized Mixed Yule Coalescent (GMYC) and Poisson Tree Processes (PTP) using partialcox1,rbcL,5.8S + ITS2,ITS1 + 5.8S + ITS2 markers to delineate species and compare to published polyphasic identification data (morphological features, phylogeny and sexual reproductive isolation) to test the resolution of these methods. ASAP, ABGD, SPNA and PTP models resolved species ofEunotia,Seminavis, Nitzschia, SellaphoraandPseudo‐nitzschiacorresponding to previous polyphasic identification, including reproductive isolation studies. In most cases, these models identified diatom species in similar ways, regardless of sequence fragment length. GMYC model presented smallest number of results that agreed with previous published identification. Following the recommendations for proper use of each model presented in the present study, these models can be useful tools to identify cryptic or closely related species of diatoms, even when the datasets have relatively few sequences.
The phospho‐regulated amphiphysin/endophilin interaction is required for synaptic vesicle endocytosis
Authors: Kontaxi Christiana , Kim Nawon , Cousin Michael A. 
Published: May 2023

AbstractThe multidomain adaptor protein amphiphysin‐1 (Amph1) is an important coordinator of clathrin‐mediated endocytosis in non‐neuronal cells and synaptic vesicle (SV) endocytosis at central nerve terminals. Amph1 contains a lipid‐binding N‐BAR (Bin/Amphiphysin/Rvs) domain, central proline‐rich (PRD) and clathrin/AP2 (CLAP) domains, and a C‐terminal SH3 domain. Amph1 interacts with both lipids and proteins, with all of these interactions required for SV endocytosis, with the exception of the Amph1 PRD. The Amph1 PRD associates with the endocytosis protein endophilin A1, however, the role of this interaction in SV endocytosis has not been investigated. In this study, we set out to determine whether the Amph1 PRD and its interaction with endophilin A1 was essential for efficient SV endocytosis at typical small central synapses. To achieve this, domain‐specific interactions of Amph1 were validated using in vitro GST pull‐down assays, with the role of these interactions in SV endocytosis determined in molecular replacement experiments in primary neuronal culture. Using this approach, we confirmed important roles for CLAP and SH3 domain interactions of Amph1 in the control of SV endocytosis. Importantly, we identified the interaction site for endophilin A1 within the Amph1 PRD and exploited specific binding mutants to reveal a key role for this interaction in SV endocytosis. Finally, we determined that the formation of the Amph1‐endophilin A1 complex is dependent on the phosphorylation status of Amph1‐S293 within the PRD and that the phosphorylation status of this residue is essential for efficient SV regeneration. This work, therefore, reveals a key role for the dephosphorylation‐dependent Amph1‐endophilin A1 interaction in efficient SV endocytosis.image
Authentic allyship for gender minorities
Authors: Gilmore John P. , Dainton Marissa , Halpin Noah 
Published: May 2023

AbstractThe visibility and discussion on the rights and needs of Trans and Non‐Binary communities in relation to healthcare have seen growing prominence in recent years. Despite an overall improvement in access to legal protections, civil rights, and in many jurisdictions specialist provision of healthcare for gender minorities, there remain poorer health outcomes in many areas and ongoing experiences of discrimination and transphobia. In this article, we set out the prerogative for nurses to step up as authentic allies for Trans and Non Binary people and put forward strategies to enhance the experience of gender minorities in healthcare through practice, education, and systems change.
How to estimate the sarcomere size based on oblique sections of skeletal muscle
Authors: Boschi Federico 
Published: May 2023

AbstractUltrastructural analysis of muscular biopsy is based on images of longitudinal sections of the fibers. Sometimes, due to experimental limitations, the resulting sections are instead oblique, and no accurate morphological information can be extracted with standard analysis methods. Thus, the biopsy is performed again, but this is too invasive and time‐consuming. In this study, we focused our attention on the sarcomere's shape and we investigated which is the structural information that can be obtained from oblique sections. A routine was written in MATLAB to allow the visualization of how a sarcomere's section appears in ultrastructural images obtained by Transmission Electron Microscopy (TEM) at different secant angles. The routine was used also to analyze the intersection between a cylinder and a plane to show how the Z‐bands and M‐line lengths vary at different secant angles. Moreover, we explored how to calculate sarcomere's radius and length as well as the secant angle from ultrastructural images, based only on geometrical considerations (Pythagorean theorem and trigonometric functions). The equations to calculate these parameters starting from ultrastructural image measurements were found. Noteworthy, to obtain the real sarcomere length in quasi‐longitudinal sections, a small correction in the standard procedure is needed and highlighted in the text. In conclusion, even non‐longitudinal sections of skeletal muscles can be used to extrapolate morphological information of sarcomeres, which are important parameters for diagnostic purposes.
Physical activity trajectories and their determinants in patients with chronic obstructive pulmonary disease: A cohort study
Authors: An Ran , Zhang Shifang , Huang Xiuxiu , Lan Yue , Cao Ting , Wan Qiaoqin 
Published: May 2023

AbstractAims and ObjectivesThe aim was to identify latent trajectories in physical activity (PA) and their determinants in adults with chronic obstructive pulmonary disease (COPD) based on the socio‐ecological model.BackgroundPA has been linked to poor long‐term outcomes in patients with COPD. However, few studies have explored their PA trajectories and their predictors.DesignCohort study.MethodsWe used data from a national cohort and included 215 participants. PA was quantified using a short PA questionnaire, and group‐based trajectory modelling was used to explore the PA trajectories. Multinomial logistic regression was conducted to identify the predictors of PA trajectories. Generalised linear mixed models were used to elucidate the associations between predictors and PA during follow‐up. A STROBE checklist was used to guide the reporting of this study.ResultsThree PA trajectory patterns were identified among 215 COPD participants with an average age of 60.51 ± 8.87: stable inactive group (66.7%), sharp decline group (25.7%) and stable active group (7.5%). The logistic regression showed that age, sex, income, peak expiratory flow, upper limb capacity, depressive symptoms, the frequency of contact with children were PA predictors. Upper limb capacity weakness and depressive symptoms were found to be associated with a sharp decline in PA during follow‐up.ConclusionsThis study revealed three PA trajectories among patients with COPD. In addition to strengthening the physical functions and mental health of patients, support from the family, community and society also play a crucial role in promoting PA of patients with COPD.Relevance to Clinical PracticeIt is essential to identify distinct PA trajectories in patients with COPD to develop future interventions that promote PA.No Patient or Public ContributionA national cohort study was used and no patients or the public were involved in the design and implementation of this study.
Employee Costs of Corporate Bankruptcy
Authors: GRAHAM JOHN R. , KIM HYUNSEOB , LI SI , QIU JIAPING 
Published: May 2023

ABSTRACTAn employee's annual earnings fall by 13% in the first full calendar year after her firm's bankruptcy, and the present value of lost earnings from bankruptcy to six years following bankruptcy is 87% of pre‐bankruptcy annual earnings. More worker earnings are lost in thin labor markets and among small firms. Ex ante compensating wage differentials for this “bankruptcy risk” are up to 2% of firm value for a firm whose credit rating falls from AA to BBB, comparable in magnitude to debt tax benefits. Thus, wage premia for expected costs of bankruptcy are sufficiently large to be an important consideration in capital structure decisions.
Retail Derivatives and Sentiment: A Sentiment Measure Constructed from Issuances of Retail Structured Equity Products
Authors: HENDERSON BRIAN J. , PEARSON NEIL D. , WANG LI 
Published: May 2023

ABSTRACTWe use retail structured equity product (SEP) issuances to construct a new sentiment measure for large capitalization stocks. The SEP sentiment measure predicts negative abnormal returns on the SEP reference stocks based on a variety of factor models, and also predicts returns in Fama‐MacBeth regressions that include a wide range of covariates. Consistent with our interpretation that SEP issuances reflect investor sentiment, aggregate SEP issuances are highly correlated with the Baker‐Wurgler sentiment index. Tobit regressions reveal that proxies for attention and sentiment predict SEP issuance volumes, providing additional evidence consistent with the hypothesis that SEP issuances reflect sentiment.