bluuebunny/crossref_metadata_2025_split huggingface dataset:
Deep transfer learning‐based approach for detection of cracks on eggs
Authors: Botta Bhavya , Datta Ashis Kumar 
Published: July 2023

AbstractEggs are the most nutrient‐rich and protein‐dense food that is affordable and consumed by most of the population. But cracks on the eggshells can pave the way for microbial contamination of the eggs posing threat to the health of the consumers. Therefore, it is crucial for the egg industries to check for the quality of eggs before reaching consumers to avoid outbreaks. Automatic visual inspection is one of the techniques that has gained popularity in the field of eggshell crack identification because of the advancements in hardware and deep learning. However, due to the limited training data, classifying eggs using eggshell crack images is challenging. Therefore, deep transfer learning was employed in this study using a pre‐trained DenseNet121 architecture in three ways—fine‐tuning (FT), feature extraction, and training deep features on machine learning classifiers. The results revealed that the FT technique performed best among the three approaches with 98.38% accuracy.Practical ApplicationsDeep transfer learning‐based techniques in food quality assessments are seeing a rise in the food industry. Eggshell crack detection is one of the challenges that can be addressed using the transfer learning approach. The purpose of this study was to investigate the effects of different transfer learning approaches in classifying the image patches extracted from egg images and determine the most effective technique to identify cracks on eggs, despite the difficulty of the classification task using a small data set of egg images. These results can promote the application of transfer learning in non‐destructive egg quality evaluation in the egg processing industries.
Barriers to optimal breastfeeding of medically complex children in the UK paediatric setting: a mixed methods survey of healthcare professionals
Authors: Hookway Lyndsey , Brown Amy 
Published: July 2023

AbstractBackgroundBreastfeeding is indisputably significant for infants and children, as well as their mothers. However, when breastfed children are admitted to the paediatric ward, they may experience breastfeeding modification due to clinical challenges, lack of staff training and institutional barriers. Although previous research has identified multiple barriers to optimal feeding within the maternity, neonatal and community settings, we know less about the barriers that exist in paediatrics. Paediatric healthcare staff attitudes, training and awareness as well as ward culture are likely to have an impact on the experiences of families but are relatively unresearched in the paediatric setting, especially with regard to the multidisciplinary nature of clinical paediatric care. The aim of this study was to explore the attitudes, awareness and perceived barriers to effective breastfeeding support provision, as well as healthcare professional perception of barriers for families.MethodsThis was a mixed methods study utilising a questionnaire which was completed by 409 healthcare professionals who identified as working within the paediatric setting. It included responses from paediatricians of all training grades, nurses, healthcare assistants and allied health professionals. Qualitative data from professionals were analysed to develop themes around professional and perceived parent barriers to optimal breastfeeding.ResultsThis study explored the barriers experienced by staff, as well as professionals' perception of parent barriers. These included lack of knowledge of how to help, a default formula culture, the pressure of maintaining strict fluid balance and breastfeeding being a low priority in the face of critical illness. Of the paediatric departments represented by this sample of healthcare professionals, most had a relatively poor culture of supporting breastfeeding, with multiple institutional barriers identified. Most professionals felt that there was not enough support for breastfeeding families on an average shift, and a large proportion identified as being one of just a few breastfeeding advocates on their ward.ConclusionIn this relatively motivated and experienced sample of healthcare professionals, there were many identified barriers to optimal breastfeeding which could potentially be addressed by training that is carefully nuanced for the paediatric population. Ensuring that paediatric multidisciplinary healthcare professionals have sufficient skills and knowledge would address the identified challenge of poor awareness of breastfeeding and of how to overcome clinical lactation obstacles. Further improvements at policy level need to address the systemic lack of resourcing in paediatrics, as well as the lack of embedded breastfeeding‐friendly organisational structures.
The Credit‐Card‐Services Augmented Divisia Monetary Aggregates*
Authors: BARNETT WILLIAM A. , CHAUVET MARCELLE , LEIVA‐LEON DANILO , SU LITING 
Published: July 2023

AbstractWhile credit cards provide transaction services, they have never been included in measures of money supply. We derive the theory to measure the joint services of credit cards and money and propose two measures of their joint services: one based on microeconomic structural aggregation theory, providing an aggregated variable within the macroeconomy; the other a credit‐card‐extended aggregate, optimized as an indicator to capture the contributions of monetary and credit card as nowcasting indicator of nominal GDP. The inclusion of the new aggregates yields substantially more accurate nowcasts of nominal GDP, illustrating the usefulness of the information contained in credit cards.
(Out)Caste language ideologies: Intersectional raciolinguistic stigma and assimilation from denotified tribal students' perspectives in rural India
Authors: Chandras Jessica 
Published: July 2023

AbstractIn India, where elementary education is a fundamental right, significant barriers stall the attainment of educational equity through linguistic inclusivity. This article explores student identities by examining intersections of language, caste, race, and socioeconomic class to make visible complexities of privilege, discrimination, and connections between social structure, identity, and language in education. From qualitative ethnographic research with a settled nomadic Tribal community in rural India, this article highlights contradictions between recent mother tongue language education policies and experiences of social disparities and stigma for Banjara Tribal youth, who speak a different mother tongue language than the language of education. I draw together theories of intersectionality and raciolinguistics to show how co‐naturalized lines of language and identity are navigated through language ideologies to shift positions of Banjara student identities. Through learning Marathi, the regional lingua franca, students transform their marginalized sociolinguistic positions into mainstream aspirants of academic and social success.
Two‐Eyed Seeing as a strategic dichotomy for decolonial nursing knowledge development and practice
Authors: McFadden Alysha , Lynam M. Judith , Hawkins Lorelei 
Published: July 2023

AbstractThe profession of nursing has recognized the need for contextual and relational frameworks to inform knowledge development. Two‐Eyed Seeing is a framework developed by Mi'kmaw Elders to respectfully engage with Indigenous and non‐Indigenous knowledges. Some scholars and practitioners, however, are concerned that Two‐Eyed Seeing re‐instantiates dichotomized notions regarding Western and Indigenous knowledges. As dichotomies and binaries are often viewed as polarizing devices for nursing knowledge development, this paper explores the local worldviews in which Two‐Eyed Seeing emerged, proposing that the onto‐epistemological and axiological ‘roots’ of the framework are antithetical to divisiveness, paradoxically asserting space for the dichotomy to stand. Two‐Eyed Seeing, if understood as a relational, decolonial praxis, could fundamentally change the way nursing scholarship and practice operate by facilitating space for diverse knowledges, ways of being, doing and relating. In this paper, considerations for nursing scholarship and practice, as well as recommendations to support the uptake of Two‐Eyed Seeing are explored. The authors assert that conceptual divisiveness, dichotomization and exclusion can be mitigated if nursing is informed by contextual knowledge, seeks to enact accountable partnerships with Indigenous knowledge holders, and holds the Mi'kmaq worldview upon which the concept developed in positive regard.
Computer‐assisted analysis of chaos in a three‐species food chain model
Authors: Hua Duo , Liu Xingbo 
Published: July 2023

AbstractIn this paper, a three‐species food chain model with Holling type IV and Beddington–DeAngelis functional responses is formulated. Numerical simulations show that this system can generate chaos for some parameter values. But the mechanism behind chaos is still unclear only through numerical simulations. Then, using the topological horseshoe theories and Conley–Moser conditions, we present a computer‐assisted analysis to show the chaoticity of this system in the topological sense, that is, it has positive topological entropy. We prove that the Poincaré map of this model possesses a closed uniformly hyperbolic chaotic invariant set, and it is topologically conjugate to a 2‐shift map. At last, we consider the impact of fear on this three‐species model. It is an important factor in controlling chaos in biological models, which has been validated in other models.
How to … define clinical education research terminology: A glossary
Authors: Finn Gabrielle M. , Charmer Ben , Burton Oliver E. , Asif Aqua , Byrne Matthew H. V. , Brennan Nicola , Brown Megan E. L. ,   the NIHR Incubator for Clinical Education Research
Published: July 2023

AbstractClinical education research (ClinEdR) utilises diverse terminology, which can lead to confusion. A common language is essential for enhancing impact. An expert panel drawn from various workstreams within the National Institute for Health and Care Research (NIHR) Incubator for Clinical Education Research was tasked with reviewing an initial list of terms for the development of a glossary of terms in the field of ClinEdR. The glossary was populated with terms, definitions and foundational papers by the authors and peer‐reviewed for accuracy. The glossary of terms developed for ClinEdR should enable researchers to use a common language, promoting consistency and improving communication. We anticipate this will be useful for ClinEdR students and early career researchers. The glossary could be integrated into educational research methods courses in ClinEdR, and through critical and reflective use, enhance the quality and subsequent impact of ClinEdR.
Fungal mechanisms of intracellular survival: what can we learn from bacterial pathogens?
Authors: Stuckey Peter V. , Santiago-Tirado Felipe H. 
Published: July 2023

ABSTRACT

Fungal infections represent a major, albeit neglected, public health threat with serious medical and economic burdens globally. With unacceptably high mortality rates, invasive fungal pathogens are responsible for millions of deaths each year, with a steadily increasing incidence primarily in immunocompromised individuals. The poor therapeutic options and rise of antifungal drug resistance pose further challenges in controlling these infections. These fungal pathogens have adapted to survive within mammalian hosts and can establish intracellular niches to promote survival within host immune cells. To do that, they have developed diverse methods to circumvent the innate immune system attack. This includes strategies such as altering their morphology, counteracting macrophage antimicrobial action, and metabolic adaptation. This is reminiscent of how bacterial pathogens have adapted to survive within host cells and cause disease. However, relative to the great deal of information available concerning intracellular bacterial pathogenesis, less is known about the mechanisms fungal pathogens employ. Therefore, here we review our current knowledge and recent advances in our understanding of how fungi can evade and persist within host immune cells. This review will focus on the major fungal pathogens, including
Cryptococcus neoformans, Candida albicans
, and
Aspergillus fumigatus
, among others. As we discover and understand the strategies used by these fungi, similarities with their bacterial counterparts are becoming apparent, hence we can use the abundant information from bacteria to guide our studies in fungi. By understanding these strategies, new lines of research will open that can improve the treatments of these devastating fungal diseases.
Upregulation of IQGAP2 by EBV transactivator Rta and its influence on EBV life cycle
Authors: Lin Kai-Min , Weng Li-Fang , Chen Shi-Yo Jill , Lin Sue-Jane , Tsai Ching-Hwa 
Published: July 2023

ABSTRACT

Epstein-Barr virus (EBV) is a human oncogenic γ-herpesvirus that establishes persistent infection in more than 90% of the world’s population. EBV has two life cycles, latency and lytic replication. Reactivation of EBV from latency to the lytic cycle is initiated and controlled by two viral immediate-early transcription factors, Zta and Rta, encoded by
BZLF1
and
BRLF1
, respectively. In this study, we found that IQGAP2 expression was elevated in EBV-infected B cells and identified Rta as a viral gene responsible for the IQGAP2 upregulation in both B cells and nasopharyngeal carcinoma cell lines. Mechanistically, we showed that Rta increases IQGAP2 expression through direct binding to the Rta-responsive element in the IQGAP2 promoter. We also demonstrated the direct interaction between Rta and IQGAP2 as well as their colocalization in the nucleus. Functionally, we showed that the induced IQGAP2 is required for the Rta-mediated Rta promoter activation in the EBV lytic cycle progression and may influence lymphoblastoid cell line clumping morphology through regulating E-cadherin expression.

IMPORTANCE
Elevated levels of antibodies against EBV lytic proteins and increased EBV DNA copy numbers in the sera have been reported in patients suffering from Burkitt’s lymphoma, Hodgkin’s lymphoma, and nasopharyngeal carcinoma, indicating that EBV lytic cycle progression may play an important role in the pathogenesis of EBV-associated diseases and highlighting the need for a more complete mechanistic understanding of the EBV lytic cycle. Rta acts as an essential transcriptional activator to induce lytic gene expression and thus trigger EBV reactivation. In this study, scaffolding protein IQGAP2 was found to be upregulated prominently following EBV infection via the direct binding of Rta to the RRE in the IQGAP2 promoter but not in response to other biological stimuli. Importantly, IQGAP2 was demonstrated to interact with Rta and promote the EBV lytic cycle progression. Suppression of IQGAP2 was also found to decrease E-cadherin expression and affect the clumping morphology of lymphoblastoid cell lines.
Differences in type 2 diabetes risk between East, South, and Southeast Asians living in Singapore: the multi-ethnic cohort
Authors: Seah Jowy Yi Hoong , Sim Xueling , Khoo Chin Meng , Tai E Shyong , van Dam Rob M 
Published: July 2023

IntroductionProspective data on differences in type two diabetes (T2D) risk between Asian ethnic groups are sparse. We, therefore, compared T2D risk for East (Chinese), South (Indian), and Southeast (Malay) Asians and examined biological factors that may contribute to ethnic differences.Research design and methodsWe included 7427 adults of Chinese, Malay, and Indian origin participating in the Singapore multi-ethnic cohort. Information on sociodemographic, lifestyle, and biological risk factors (body mass index (BMI), waist circumference, blood lipids, blood pressure, C reactive protein, adiponectin, and homeostasis model assessment for insulin resistance and beta-cell function) were collected using standardized interviews and physical examinations. T2D cases were based on physician diagnoses, a national medical registry, fasting plasma glucose, or glycated hemoglobin A1c. We used multivariable logistic association and mediation analyses.ResultsDuring an average follow-up of 7.2 years (SD 2.2 years), we documented 595 cases of incident diabetes. Ethnic Malays (OR 2.08, 95% CI 1.69 to 2.56) and Indians (OR 2.22, 95% CI 1.80 to 2.74) had an approximately twofold higher risk of T2D compared with ethnic Chinese. Higher BMI explained the higher risk for Malay compared with Chinese ethnicity. Higher BMI, waist circumference, inflammation, and insulin resistance, and lower beta-cell function and high-density lipoprotein-cholesterol significantly contributed to the higher T2D risk for Indian compared with Chinese ethnicity. However, part of the higher T2D risk associated with Indian ethnicity remained unexplained. Despite their lower diabetes risk, Chinese participants had the lowest adiponectin levels.ConclusionsDifferent Asian ethnic groups have unique biological risk factor profiles related to T2D development that may warrant targeted approaches for prevention and treatment.
Blood pressure medication and acute kidney injury after intracerebral haemorrhage: an analysis of the ATACH-II trial
Authors: Naidech Andrew M , Wang Hanyin , Hutch Meghan , Murphy Julianne , Paparello James , Bath Philip , Srivastava Anand , Luo Yuan 
Published: July 2023

BackgroundAcute blood pressure (BP) reduction is standard of care after acute intracerebral haemorrhage (ICH). More acute BP reduction is associated with acute kidney injury (AKI). It is not known if the choice of antihypertensive medications affects the risk of AKI.MethodsWe analysed data from the ATACH-II clinical trial. AKI was defined by the Kidney Disease: Improving Global Outcomes criteria. We analysed antihypertensive medication from two sources. The first was a case report form that specified the use of labetalol, diltiazem, urapidil or other. We tested the hypothesis that the secondary medication was associated with AKI with χ2test. Second, we tested the hypotheses the dosage of diltiazem was associated with AKI using Mann-Whitney U test.ResultsAKI occurred in 109 of 1000 patients (10.9%). A higher proportion of patients with AKI received diltiazem after nicardipine (12 (29%) vs 21 (12%), p=0.03). The 95%ile (90%–99% ile) of administered diltiazem was 18 (0–130) mg in patients with AKI vs 0 (0–30) mg in patients without AKI (p=0.002). There was no apparent confounding by indication for diltiazem use.ConclusionsThe use of diltiazem, and more diltiazem, was associated with AKI in patients with acute ICH.
Growth rates and histopathological outcomes of small (6–9 mm) colorectal polyps based on CT colonography surveillance and endoscopic removal
Authors: Pooler B Dustin , Kim David H , Matkowskyj Kristina A , Newton Michael A , Halberg Richard B , Grady William M , Hassan Cesare , Pickhardt Perry J 
Published: July 2023

Background and aimsThe natural history of small polyps is not well established and rests on limited evidence from barium enema studies decades ago. Patients with one or two small polyps (6–9 mm) at screening CT colonography (CTC) are offered CTC surveillance at 3 years but may elect immediate colonoscopy. This practice allows direct observation of the growth of subcentimetre polyps, with histopathological correlation in patients undergoing subsequent polypectomy.DesignOf 11 165 asymptomatic patients screened by CTC over a period of 16.4 years, 1067 had one or two 6–9 mm polyps detected (with no polyps ≥10 mm). Of these, 314 (mean age, 57.4 years; M:F, 141:173; 375 total polyps) elected immediate colonoscopic polypectomy, and 382 (mean age 57.0 years; M:F, 217:165; 481 total polyps) elected CTC surveillance over a mean of 4.7 years. Volumetric polyp growth was analysed, with histopathological correlation for resected polyps. Polyp growth and regression were defined as volume change of ±20% per year, with rapid growth defined as +100% per year (annual volume doubling). Regression analysis was performed to evaluate predictors of advanced histology, defined as the presence of cancer, high-grade dysplasia (HGD) or villous components.ResultsOf the 314 patients who underwent immediate polypectomy, 67.8% (213/314) harboured adenomas, 2.2% (7/314) with advanced histology; no polyps contained cancer or HGD. Of 382 patients who underwent CTC surveillance, 24.9% (95/382) had polyps that grew, while 62.0% (237/382) remained stable and 13.1% (50/382) regressed in size. Of the 58.6% (224/382) CTC surveillance patients who ultimately underwent colonoscopic resection, 87.1% (195/224) harboured adenomas, 12.9% (29/224) with advanced histology. Of CTC surveillance patients with growing polyps who underwent resection, 23.2% (19/82) harboured advanced histology vs 7.0% (10/142) with stable or regressing polyps (OR: 4.0; p<0.001), with even greater risk of advanced histology in those with rapid growth (63.6%, 14/22, OR: 25.4; p<0.001). Polyp growth, but not patient age/sex or polyp morphology/location were significant predictors of advanced histology.ConclusionSmall 6–9 mm polyps present overall low risk to patients, with polyp growth strongly associated with higher risk lesions. Most patients (75%) with small 6–9 mm polyps will see polyp stability or regression, with advanced histology seen in only 7%. The minority of patients (25%) with small polyps that do grow have a 3-fold increased risk of advanced histology.
Targeting MS4A4A on tumour-associated macrophages restores CD8+ T-cell-mediated antitumour immunity
Authors: Li Yongsheng , Shen Zhiyong , Chai Zhen , Zhan Yizhi , Zhang Yaowei , Liu Zhengyu , Liu Yuechen , Li Zhenkang , Lin Mingdao , Zhang Zhanqiao , Liu Wei , Guan Shenyuan , Zhang Jinchao , Qian Junying , Ding Yi , Li Guoxin , Fang Yuan , Deng Haijun 
Published: July 2023

ObjectiveCheckpoint immunotherapy unleashes T-cell control of tumours but is suppressed by immunosuppressive myeloid cells. The transmembrane protein MS4A4A is selectively highly expressed in tumour-associated macrophages (TAMs). Here, we aimed to reveal the role of MS4A4A+TAMs in regulating the immune escape of tumour cells and to develop novel therapeutic strategies targeting TAMs to enhance the efficacy of immune checkpoint inhibitor (ICI) in colorectal cancer.DesignThe inhibitory effect of MS4A4A blockade alone or combined with ICI treatment on tumour growth was assessed using murine subcutaneous tumour or orthotopic transplanted models. The effect of MS4A4A blockade on the tumour immune microenvironment was assessed by flow cytometry and mass cytometry. RNA sequencing and western blot analysis were used to further explore the molecular mechanism by which MS4A4A promoted macrophages M2 polarisation.ResultsMS4A4A is selectively expressed by TAMs in different types of tumours, and was associated with adverse clinical outcome in patients with cancer. In vivo inhibition of MS4A4A and anti-MS4A4A monoclonal antibody treatment both curb tumour growth and improve the effect of ICI therapy. MS4A4A blockade treatment reshaped the tumour immune microenvironment, resulting in reducing the infiltration of M2-TAMs and exhausted T cells, and increasing the infiltration of effector CD8+T cells. Anti-MS4A4A plus anti-programmed cell death protein 1 (PD-1) therapy remained effective in large, treatment-resistant tumours and could induce complete regression when further combined with radiotherapy. Mechanistically, MS4A4A promoted M2 polarisation of macrophages by activating PI3K/AKT pathway and JAK/STAT6 pathway.ConclusionTargeting MS4A4A could enhance the ICI efficacy and represent a new anticancer immunotherapy.