bluuebunny/crossref_metadata_2025_split huggingface dataset:
War on telomere: Telomere shortening in War-Exposed Syrian Refugees
Authors: cam fethi sirri , Rahman Fatih , Batır Muhammet Burak 
Published: December 2022

Abstract
A telomere is a region of repetitive nucleotide sequences at each end of a chromosome, which protects the end of the chromosome. The cellular environment plays an essential role in regulating telomere length. Also, there is growing evidence that harsh conditions in the environment of humans increase the telomere shortening level. In this study, the effects of exposure to war and the duration of exposure to war on telomere length were investigated. In addition, the anxiety and depression status of the participants and the effect of anxiety and depression on telomere length were also examined. A group of 40 cases exposed to war and had to migrate to Turkey, and a control group of 40 who were not exposed to war were examined according to their anxiety and depression status. They were evaluated according to the Hospital and Anxiety Score (HADS). Also, the between the war exposed and control groups were evaluated according to their telomere length. The telomere length of the samples was analyzed with Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR) method. While the telomere length of the group exposed to war was 8.27 ± 0.68 kb, the telomere length of the control group was 8.96 ± 0.43 kb. In addition, a significant relationship was found between increased exposure to war and the shortening of telomeres. This study showed a significant relationship between war exposure and telomere shortening.
Shengjiang San alleviated sepsis-induced lung injury through its bidirectional regulatory effect
Authors: Shifan Yan , Yu JIANG , Ting Yu , Changmiao Hou , Wen Xiao , Jing Xu , Huili Wen , Jingjing Wang , Shutong Li , Fang Chen , Shentang Li , Xiehong Liu , Hao Tan , Liu yanjuan , Yimin zhu 
Published: December 2022

Abstract
Background: Sepsis is a life-threatening organ dysfunction caused by dysregulated host responses to infection, for which effective therapeutic strategies are still absent. Shengjiang San (SJS), a well-known Traditional Chinese Medicine formula, has been widely used clinically. However, its role in sepsis-induced lung injury remains unclear.
Methods: To explore its specific mechanism, we firstly established a sepsis animal model using cecal ligation and puncture (CLP) and treated MH-S cells with LPS plus ATP. Then, UPLC/Q-TOF-MS/MS was utilized to identify its active ingredients. Network pharmacology analysis was performed to uncover the potential mechanism. HE staining and biochemical analysis were conducted to validate its therapeutic effect. ELISA was applied to detect the release of pro-inflammatory and anti-inflammatory cytokines. Western blot was utilized to detect the protein levels of GSDMD, NLRP3, P65, ASC and caspase-1.
Results: SJS could dramatically increase the survival rate of sepsis. In addition, it is able to inhibit the pro-inflammatory cytokines release at day 1 post CLP while promote their production at day 7, indicating SJS could attenuate uncontrolled inflammatory response in the early stage and improve immunosuppression in the late phase. Network pharmacology analysis showed that pyroptosis is the crucial action SJS exerted in the protection of sepsis-induced lung injury. Western blot data implicated SJS could attenuate pyroptosis in early sepsis while enhance in the late phase.
Conclusions: SJS acted to alleviate sepsis-induced lung injury through its bidirectional regulatory effect.
The Effect of Intradialytic Exercise on Dialysis Patient Survival: A Randomized Controlled Trial
Authors: Tabibi Mohammad Ali , Cheema Bobby , Salimian Nasrin , Corrêa Hugo , Ahmadi Saghar 
Published: December 2022

Abstract
Background
Patients with kidney failure have a high mortality rate. This study aimed to evaluate the effect of intradialytic exercise on survival in patients receiving hemodialysis (HD).
Methods
In this randomized controlled trial conducted in a HD center in Iran, adult patients receiving chronic HD were randomized to intradialytic exercise (60 minutes) in the second hour of thrice weekly dialysis for 6 months (intervention) or no intradialytic exercise (control). The primary outcome was survival rate at 12 months. Secondary outcomes were serum albumin, hemoglobin, hematocrit, red blood cell count, serum calcium, serum phosphorous, parathyroid hormone, physical function (6-minute walk test) and nutritional status (Geriatric Nutritional Risk Index) during the first 6 months. The trial follow-up period was 12 months.
Results
The study included 74 participants (44 males) with an age average of 64 ± 12 years old and a dialysis history of 27 ± 12 months, randomized to intervention (n = 37) or control (n = 37). Compared with controls, 1-year survival was higher in the intervention group compared with the control group (94% vs 73%, P = 0.01). The hazard ratio on univariate analysis in intervention group was 0.17 (95% CI 0.04–0.8; P = 0.02) compared to that in control group. During the 6-month intervention period, significant between-group changes were observed in all secondary outcomes between the intervention and control groups.
Conclusion
Intradialytic exercise performed for at least 60 minutes during thrice weekly dialysis sessions improves survival in adult patients receiving HD. This effect may be mediated by exercise-related improvements in bone mineral metabolism, anemia, physical function or nutritional status.
Trial registration
ClinicalTrials.gov Identifier: NCT04898608. Retrospectively registered on 24/05/2021. Registered trial name: The Effect of Intradialytic Exercise on Dialysis Patients Survival.
Effects of social influences on infant food consumption in the city of Ouagadougou (Burkina Faso): a retrospective survey
Authors: BOUGMA Sanogo , TAPSOBA François , SEMPORE Judith Nomwendé , BOUGMA Sibiri , DOUNIA Prudence , SONGRE-OUATTARA Laurencia Toulsoumdé , SAVADOGO Aly 
Published: December 2022

Abstract

Background
From the age of 6 months, children need, in addition to breast milk, a complementary food with nutritional composition that meets their needs. In addition, the lack of adaptation of some children to family feeding conditions could be sources of malnutrition. In Burkina Faso, little information’s are available on children's consumption of family foods. The objective was therefore to describe the feeding habits and frequencies of infants aged from 6 to 23 months.
Methods
The study consisted in collecting through a retrospective survey and analyzing dietary data of the last 24 hours. Sphinx V5, IBM SPSS Statistics 20.0 and XLSTAT 2016 were used to process the data.
Results
The dietary data of 618 children were collected. It was found that the most consumed foods were simple porridges (67.48%), cooked cereal doughs (65.70%), cookies and cakes (62.94%), juices and sweetened drinks (62.94%). The least consumed foods were cowpeas and peas (17.31%), improved porridges (13.92%) and eggs (6.63%). The most observed meal frequency was three meals per day (33.98%). Children having the minimum meal frequency per day was 86.41%. The principal component analysis showed a positive correlation between the consumption of imported infant flours, fish soups, fruits, juices and sweetened drinks, cookies and cakes, simple porridges, cooked cereal doughs with the social status of the mother. In addition, 55.72% of the children showed a preference for local infant formula porridges. The main reason given by responding mothers whose children did not consume infant formula was lack of information (57.75%).
Conclusion
In general, a high consumption of family-type meals with acceptable meal frequencies has been observed. In addition, a correlation between the types of food consumed and the social status of the parents was also found.
Analysis of the crosstalk between EMT-related gene and Tumor-infiltrating immune cell in Esophageal Squamous Cell Carcinoma
Authors: Wu Bin , Li Shihao , Zhai Yujia , Li Zihao , Li Feng , Dong Bo , Sheng Yinliang , Huang Lan , Qi Yu 
Published: December 2022

Abstract
Background: Esophageal squamous cell carcinoma (ESCC) has the leading rate of metastasis and mortality, so effectively suppressing cancer progression and prolonging the long-term prognosis has always been a proactive and challenging task.
Methods: This study aimed to identify the promising biomarkers related to epithelial-mesenchymal transition (EMT) and attempted to elucidate the potential role of the crosstalk between the EMT process and tumor infiltrating immune cell in the tumorigenesis and progression of ESCC. Based on the combined analysis of mouse esophageal cancer models and ESCC patients, we identified 5 novel EMT biomarkers (PLAUR, TIMP1, LAMC2, COL7A1 and IGFBP3), which are significantly up-regulated during ESCC tumorigenesis. Based on the expression of these 5 genes, ESCC patients were clustered into three subgroups (cluster 1, cluster 2 and cluster 3).
Results: Notably, compared to cluster 2, patients in cluster 3 had higher expression of EMT-related genes (ZEB1, Vimentin, Snail, and Slug), higher infiltration of myeloid dendritic cells and M2 macrophages, and lower infiltration of T regulatory cells (Tregs). Moreover, we also demonstrated that ESCC patients with high infiltration of Tregs had lower expression of Vimentin and Snail, while patients with high infiltration of M2 macrophage had higher expression of ZEB1, Vimentin and Snail. Finally, through multi-cohort correlation analysis and verification, we identified 4 core biomarker-related ceRNA networks (LAMC2-MIR193BHG axis, LAMC2-MIR4435-2HG axis, LAMC2-PLAUR axis and PLAUR-LINC00707 axis).
Conclusions: In summary, we identified and initially validated 5 EMT biomarkers, and investigated the interaction of EMT process and tumor infiltrating immune cell in ESCC.
Low-intensity transcranial ultrasound stimulation modulates neural activities in mice under propofol anesthesia
Authors: Liu Meiqi , Yuan Yi , Wang Xingran , Wang Teng , Bian Nannan , Zhao Li , Cui Guangying , Liu Wenchao , Ma Zhongfeng , Yang Xiaochun , Liang Shujuan , Liu Zhuo 
Published: December 2022

Abstract
Background: Previous study reported that low-intensity transcranial ultrasound stimulation (TUS) can significantly decrease time to emergence from anesthesia in rats. However, how TUS modulates neural activity during wakefulness in anesthetized mice is unclear.
Methods: In this study, to answer this question, we used ultrasound to stimulate the brain tissue of propofol anesthesia mice, recorded local field potentials in the mouse motor cortex and EMG signals from mouse neck, and analyzed the emergence and recovery time, the mean absolute power, relative power and entropy of local field potentials.
Results: We found that TUS can significantly reduce the time from anesthesia to awake; We also found that compared with Sham group, 20 min after TUS during the recovery from anesthesia, 1) the absolute power of local field potentials in mice was significantly reduced at [1-4 Hz] and [13-30Hz] frequency bands, and significantly increased at [55-100 Hz], [100-140 Hz] and [140-200Hz] frequency bands. 2) the relative power of local field potentials in mice enhanced at [30-45 Hz], [100-140 Hz] and [140-200 Hz] frequency bands. 3) the entropy of local field potentials ([1-200Hz]) increased.
Conclusion:These results demonstrate that TUS can effectively modulate neural activities during wakefulness in anesthetized mice andhas a positive effect on the recovery from propofol anesthesia of mice.
The potential of lncRNAs to regulate cuproptosis in hepatocellular carcinoma: establishment and validation of a novel risk model
Authors: He Jing , Li Weiqi , Shen Hao , Chang Yushun , Zhao Weijun , Liu Boqiang , He Qiang , Yu Hong , Wang Yifan , Shi Liang , Cai Xiujun 
Published: December 2022

Abstract
Background
Recently, a novel, different from the known mechanisms, copper-dependent cell death-"cuproptosis" was demonstrated. However, the regulation of cuproptosis, especially in malignancy, remains unclear. This study aimed to identify the hepatocellular carcinoma-long noncoding RNAs (HCC-lncRNAs) closely related to cuproptosis.
Methods
First, we identified lncRNAs that are associated with cuproptosis-related genes and abnormally expressed in HCC. Subsequently, the comprehensive methods of Univariate cox regression analysis, multivariate cox regression analysis and LASSO regression analysis were used to further screen targets and establish a risk models in the training group and test group with no clinical difference in TCGA. ssGSEA was used to estimate the contents of immune cells in the tumor microenvironment. In vitro elesclomol-CuCl2 shock test verified the correlation between the lncRNAs targets and copper toxicity.
Results
We successfully constructed a cuproptosis-related risk model: risk score = 0.82 * AC018690.1 + 0.65 * AL050341.2 + 0.61 * LINC02038, which could accurately reflect the prognosis of HCC patients. Further, we added clinical factors to optimize the model. Meanwhile, the tumor immune microenvironment was observably different between high and low risk groups, in which the infiltration levels of T helper 2 cells, T helper cells 17 and dendritic cells were significantly related to the risk scores. Interestingly, the patients in the high-risk group are more sensitive to immunotherapy, while sorafenib and dasatinib are less effective. Finally, we confirmed that knocking down these lncRNAs targets could significantly weaken the resistance to cuproptosis in vitro.
Conclusions
Based on three novel HCC-lncRNAs, we established an accurate risk model with potential value for clinical application. Given these lncRNAs dramatically regulate the response of HCC to copper toxicity, it raises hope for the development of copper-related therapy, even “sorafenib -copper ionophores -immunotherapy”, on HCC.
Automated sample preparation for electrospray ionization mass spectrometry based on CLOCK- controlled autonomous centrifugal microfluidics
Authors: Futami Masahiro , Naito Hiroki , Ninomiya Satoshi , Chen Lee Chuin , Iwano Tomohiko , Yoshimura Kentaro , Ukita Yoshiaki 
Published: December 2022

Abstract
We report a centrifugal microfluidic device that automatically executes sample preparation at steady-state rotation for clinical applications using mass spectrometry. The autonomous microfluidic device was designed for the control of liquid operation based on centrifugal hydrokinetics (CLOCK) paradigm. The reported device was highly stable with less than 7% variation in the time of each unit operation (sample extraction, mixing, and supernatant extraction) with respect to the preparation process. Mixing of the sample and organic solvent in this device was realized using an agitation mechanism with bubbling. We confirmed that the device effectively removed protein aggregates from the sample, and the performance was comparable to that of conventional manual sample preparation procedure that uses high-speed centrifuge. In addition, probe electrospray ionization mass spectrometry (PESI-MS) was performed to compare the device-treated and manually treated samples. The obtained PESI-MS spectra were analyzed by partial least squares discriminant analysis, confirming that the preparation capability of the device was equivalent to that of the conventional method.
CXCL9, 10, 11/CXCR3 axis contributes to the progress of primary Sjogren's syndrome by activating GRK2 to promote T lymphocyte migration
Authors: Zhang Jing , Zhang Xiao , Shi Xinjie , Liu Yuqi , Chen Danqian , Tian QIanwen , Lin Ning , Wei Wei , Wu Huaxun 
Published: December 2022

Abstract
Primary Sjogren's syndrome (pSS) is a systemic autoimmune disease that causes dysfunction of secretory glands and the specific pathogenesis is still unknown. The CXCL9, 10, 11/CXCR3 axis and G protein-coupled receptor kinase 2 (GRK2) involved in many inflammation and immunity processes. We used NOD/Ltj mice, a spontaneous SS animal model, to elucidate the pathological mechanism of CXCL9, 10, 11/CXCR3 axis promoting T lymphocyte migration by activating GRK2 in pSS. We found that CD4+GRK2, Th17+CXCR3 was apparently increased and Treg+CXCR3 was significantly decreased in the spleen of 4W NOD mice without sicca symptom compared to ICR mice (control group). The protein levels of IFN-γ, CXCL9, 10, 11 increased in submandibular gland (SG) tissue accompanied by obvious lymphocytic infiltration and Th17 cells overwhelmingly infiltrated relative to Treg cells at the sicca symptom occurs, and we found that the proportion of Th17 cells was increased, whereas that of Treg cells was decreased in spleen. In vitro, we used IFN-γ to stimulate human salivary gland epithelial cells (HSGECs) co-culture with Jurkat cells, and the results showed that CXCL9, 10, 11 was increased by IFN-γ activates JAK2/STAT1 signal pathway and Jurkat cells migration increased with the raised of cell membrane GRK2 expression. HSGECs with tofacitinib or Jurkat cells with GRK2 siRNA can reduce the migration of Jurkat cells. The results indicate that CXCL9, 10, 11 significantly increased in SG tissue through IFN-γ stimulating HSGECs, and the CXCL9, 10, 11/CXCR3 axis contributes to the progress of pSS by activating GRK2 to promote T lymphocyte migration.
Waterhouse-Friderichsen Syndrome Following a Dog Bite in an Asplenic Patient: Case Report and Review of the Literature
Authors: Berlot Giorgio , Tomasini Ariella , Zanchi Silvia , Moro Edoardo , Pinamonti Maurizio , Mohamed Sara , De Bellis Eleonora 
Published: December 2022

Abstract
The Waterhouse-Friderichsen Syndrome represents a critical condition characterized by a septic shock associated with a disseminated intravascular coagulation causing the plugging of the microvascular network virtually all organs and systems, including the skin, the kidneys, the liver and adrenal glands; the mortality rate is elevated and survivors often must undergo multiple limb amputations. Here we describe the uncommon case of an asplenic patient who developed this syndrome after a superficial wound caused by a dog bite causing an initial infection due to Capnocytophaga canimorsus that is part of the normal oral microbiome of pets. The clinical and pathological findings and the current and future therapeutic options are reviewed and discussed.
Identifying an unknown coefficient in the fractional parabolic differential equation
Authors: Sidi Hamed Ould , Huntul M. J. , Sidi Maawiya Ould , Emadifar Homan 
Published: December 2022

Abstract
In this study, we considered a fractional parabolic equation for identifying the unknown diffusion coefficient from the noisy measurement of the ultimate time solution. It is an inverse problem involving a nonlocal operator that is nonlinear and poorly formulated. By demonstrating the existence of this inverse problem singular solution with regard to the final observed data, we demonstrate the identifiability of this problem. The inverse problem is expressed as a regularized optimization problem that minimizes a cost function of the least-squares kind. We have covered various theoretical and practical difficulties pertaining to the problem under consideration. It has been demonstrated that a unique stable solution to the optimization problem exists. The Morozov discrepancy principle and the conjugate gradient approach are used to develop an iterative reconstruction procedure. The accuracy and efficiency of the suggested method are illustrated using a few numerical examples.
Transfer learning-based attenuation correction for static and dynamic cardiac PET using a generative adversarial network
Authors: Sun Hao , Wang Fanghu , Yang Yuling , Hong Xiaotong , Xu Weiping , Wang Shuxia , Mok Greta S. P. , Lu Lijun 
Published: December 2022

Abstract
Purpose
Current attenuation correction (AC) of myocardial perfusion (MP) positron emission tomography (PET) remains challenging in routine clinical practice due to the propagation of CT-based artifacts and potential mismatch between PET and CT. The goal of this work is to demonstrate the feasibility of directly generating attenuation-corrected PET (AC PET) images from non-attenuation-corrected PET (NAC PET) images in the reconstruction domain for [13N]ammonia MP PET based on a generative adversarial network (GAN).
Methods
We recruited 60 patients who underwent rest [13N]ammonia cardiac PET/CT examinations. One static frame and twenty-one dynamic frames were acquired for each patient with both NAC PET and CT-based AC (CTAC) PET images. Paired 3D static or dynamic NAC and CTAC PET images were used as network inputs and labels for static (S-DLAC) and dynamic (D-DLAC) MP PET, respectively. In addition, the pre-trained S-DLAC network was fine-tuned by 3D paired dynamic NAC and CTAC PET frames for then AC in the dynamic PET images (D-DLAC-FT). Qualitative and quantitative assessments were implemented using CTAC PET as reference.
Results
The proposed S-DLAC, D-DLAC and D-DLAC-FT methods were qualitatively and quantitatively consistent with clinical CTAC. The S-DLAC showed a higher correlation with the reference static CTAC (S-CTAC) as compared to static NAC. The estimated kinetic parameters and blood volume fraction images from D-DLAC and D-DLAC-FT methods showed comparable performances with the reference dynamic CTAC (D-CTAC). D-DLAC-FT was slightly better than D-DLAC in terms of various physical and clinical indices.
Conclusion
The proposed S-DLAC, D-DLAC and D-DLAC-FT methods reduced attenuation artifacts significantly and achieved comparable performance with clinical CTAC for static and dynamic cardiac PET. The use of transfer learning is effective for the dynamic MP PET AC purpose.
LncRNA HCG18 promotes Prostate Cancer progression by regulating miR-512-3p/HK-2 axis
Authors: Zhu Yaru , Wang Zhijing , Li Haopeng , Ren Zhen , Zi Tong , Qin Xin , Sun Wenhuizi , Chen Xi , Wu Gang 
Published: December 2022

Abstract
Long non-coding RNA (lncRNA) plays an important role in tumor progression. Numerous studies show that lncRNA is strongly associated with prostate cancer progression. Our study confirmed that lncRNA HCG18 was highly expressed in prostate cancer (PC) and correlated with tumor progression in databases and cell lines. Western blot, RNA Pull-down, dual luciferase assay and rescue assays verified the correlation among lncRNA HCG18, miR-512-3p and hexokinase-2(HK-2). In general, the results showed that lncRNA HCG18 accelerated cell proliferation, migration, and invasion of PC via up-regulating HK-2 through sponging miR-512-3p, which provided a new direction for the diagnosis and treatment of PC.
Investigation and Numerical Simulations of Openings Impact on the Workability and Performance of the Flat Slabs via Punching Shear Resistance Analysis
Authors: Ibraim Mohaimen , Naimi Sepanta 
Published: December 2022

Abstract
This research is executed to investigate and examine the critical impact of making openings and holes (needed for mechanical and electrical installations) on the flat slabs’ workability and performance. Numerical analysis and simulations using the ABAQUS software were implemented. Besides, a comparative study (between nine cases) was adopted to explore the influence of openings on the workability and performance of flat slabs. The damaged zone area, maximum load, maximum deflection, and maximum displacement were recorded. Also, the effect of the opening size on these variables was analyzed. The results revealed that cases C1, C2, and C3 have approximately similar maximum displacement values ranging between 31.6 mm and 32.7 mm. The maximum load (failure load) in case C2 (50×50 cm) was 223.7 kN, 34% less than in case C1, and case C3 (100×100 cm) was 186.5 kN, 45% less than in case C1. The damaged zone area increased by 47 percent and 144 percent for C2 and C3, respectively. It is evident that when opening sizes increase, punching shear resistance decreases because the perimeter decreases. Also, different values of loads were recorded corresponding to various amounts of displacement in cases from one to nine, in which the load increases with elevating the displacement.