bluuebunny/crossref_metadata_2025_split huggingface dataset:
Endophytic upper tract urothelial carcinoma in a solitary kidney treated by cryotherapy: an unorthodox case for successful management
Authors: Abdelaziz Ahmad , Sultan Mark , Hammad Muhammed A , Martinez Juan Ramon , Yacoub Maria , Youssef Ramy F 
Published: June 2023

Abstract
Background
Nephroureterectomy remains the gold standard treatment for upper tract urothelial carcinoma (UTUC). Considering the high risk of developing renal function impairment after surgery, the rationale for nephron sparing approaches in treatment of UTUC has been raised. In this case, renal cryoablation was able to achieve successful oncologic control while preserving renal function during 5 years of follow up without intraoperative or post operative complications.

Case presentation
A 79 year old male presents after three months of macroscopic hematuria. Imaging revealed a 3.6 × 3.1 × 2.7 cm endophytic mass in the interpolar region of the left kidney and an atrophic right kidney. After weighing the lesion’s location with the patient’s of complex medical history, he was counselled to undergo a minimally invasive percutaneous cryoablation as treatment for his solitary renal mass. A diagnostic dilemma was encountered as imaging suggested a diagnosis of renal cell carcinoma. However, the pre-ablation biopsy established an alternative diagnosis, revealing UTUC. Percutaneous cryoablation became an unorthodox treatment modality for the endophytic component of his UTUC followed by retrograde ureteroscopic laser fulguration. The patient was followed in 3 months, 6 months, then annually with cross sectional imaging by MRI, cystoscopy, urine cytology and renal function testing. After five years of follow-up, the patient did not encountered recurrence of UTUC or deterioration in renal function, thereby maintaining a stable eGFR.

Conclusion
Although evidence for nephron-sparing modalities for UTUC is mounting in recent literature, limited data still exists on cryotherapy as a line of treatment for urothelial carcinoma. We report successful management of a low-grade UTUC using cryoablation with the crucial aid of an initial renal biopsy and long-term follow-up. Our results provide insight into the role of cryoablation as a nephron-sparing approach for UTUC.
Effectiveness of the Positive Youth Development (PYD) Program on reducing aggression among high school female students
Authors: Zareei mahmoodabadi z Hassan , ebrahimi Asieh , Sooreshjani Razieh Heydari 
Published: June 2023

Abstract
Background
The positive youth development program highlights the abilities of adolescents and young people. This concept emphasizes that teenagers are capable of making positive changes (such as: flexibility, Responsibility, Identify abilities). This study aimed to determine the effectiveness of the Positive Youth Development (PYD) program on reducing aggression among high school female students in Yazd city. Iran.

Methods
This was a quasi-experimental study, using pretest-posttest design with a control group. In the 2019–2020 academic year, the statistical population comprised all female students in high school first and second years in the city of Yazd, Iran. Using purposive sampling and based on include and exclude criteria (high risk of aggression due to their scores on a screening test and Parental consent to participate in the class) 30 female students were selected. Randomly, they were assigned to an experimental(n = 15) and control groups(n-15). The Buss-Perry Aggression Questionnaire was conducted on students. The experimental group had 8 intervention sessions, whereas the control group received no intervention. The data were subsequently examined using Analysis of Covariance(ANCOVA).

Results
The dependent t-test revealed a significant difference between the pretest-posttest aggression scores in the experimental group, but there was no significant difference in the control group. There was no significant difference in the mean scores of physical aggression, anger, and hostility in the experimental and control groups, but a significant difference was found between the experimental and control groups in the mean verbal aggression scores.

Conclusion
training of positive youth development did not affect on aggression and its three dimensions (physical aggression, anger, and hostility) and only affected adolescent verbal aggression.
Promising score for teaching and learning environment: an experience of a fledgling medical college in Saudi Arabia
Authors: Almansour Mohammed , AlMehmadi Bader A. , Zeb Nida Gulzar , Matbouly Ghassan , Sami Waqas , Badahdah Al-Mamoon 
Published: June 2023

Abstract
Background
Professional competency of graduates of an institute reflects its teaching and learning environment (TLE). This study aimed to provide a preliminary assessment of the TLE at the College of Medicine at Majmaah University.

Methods
A cross-sectional survey was conducted during the 2019-20 academic year among students at the College. A validated scoring tool “the Experience of Teaching and Learning Questionnaire” (available at https://bit.ly/3sVBuEw) was used. The mean score of each section and statement, the difference between the mean scores of different demographic groups, and correlations between sections were analysed.

Results
A total of 234 (72.2%) enrolled students participated in this survey, with a male-to-female ratio and a ratio of participants from basic to clinical years being 2:1 and 1:1, respectively. Most participants reported a GPA of above 3/5. The overall mean score was 3.52/5 points. Section one “approaches to learning and studying” has the highest mean score (3.68), and no section scored a mean below three, though section three “demands made by the course” scored a borderline mean of 3.08. Students in clinical years had a significantly higher overall mean score compared to their counterparts (3.66 vs. 3.39, p < 0.001).

Conclusions
Students at the College had a positive perception of the TLE, but face challenges in coping with the demands of acquiring knowledge and subject-based skills, and in appreciating the TLE especially during basic science years, highlighting the need for an atmosphere that allows them to meet demands and develop greater appreciation.
Learning for doctor-to-doctor collaboration: a qualitative study exploring the experiences of residents and supervisors with intraprofessional workplace learning in complex tertiary care
Authors: Teheux Lara , Wollaars Hanna , Draaisma Jos M.T. , Coolen Ester H.A.J. , Kuijer-Siebelink Wietske , van der Velden Janiëlle A.E.M. 
Published: June 2023

Abstract
Background
To deliver high-quality care for individuals with complex medical conditions, residents need to be trained across the boundaries of their specialties. This study aimed to explore learning activities and influencing factors in intraprofessional workplace learning by residents in complex tertiary care.

Methods
This qualitative study was conducted in a tertiary care children’s hospital. In September – December 2017, fourteen individual and two focus group interviews were conducted with a purposive sample of residents and supervisors of various specialties. Transcribed interviews were thematically analyzed to describe learning activities and influencing factors that play a role in intraprofessional workplace learning in complex tertiary care settings during residency training.

Results
Respondents described numerous activities that they considered opportunities for intraprofessional learning, both directly and not directly related to patient care. However, deliberate attention to intraprofessional learning often seemed to be lacking in clinical practice. Influencing factors on a system (macro), organization (meso) and personal and interpersonal level (micro) level were identified. Factors on the macro and meso level mainly determined whether intraprofessional learning opportunities arose, while micro level factors mainly influenced whether opportunities were seized.

Conclusions
There are ample opportunities for intraprofessional workplace learning in complex tertiary care for residents. Residents may benefit more from intraprofessional learning opportunities if these are made more intentional and deliberate. Influencing factors at the macro, meso and micro level provide targets for interventions aimed at enhancing intraprofessional workplace learning in postgraduate medical training.
Identification of molecular subgroups in osteomyelitis induced by staphylococcus aureus infection through gene expression profiles
Authors: Shi Xiangwen , Ni Haonan , Tang Linmeng , Li Mingjun , Wu Yipeng , Xu Yongqing 
Published: June 2023

Abstract
Background
Staphylococcus aureus (S. aureus) infection-induced osteomyelitis (OM) is an inflammatory bone disease accompanied by persistent bone destruction, and the treatment is challenging because of its tendency to recur. Present study was aimed to explore the molecular subgroups of S. aureus infection-induced OM and to deepen the mechanistic understanding for molecularly targeted treatment of OM.

Methods
Integration of 164 OM samples and 60 healthy samples from three datasets of the Gene Expression Omnibus (GEO) database. OM patients were classified into different molecular subgroups based on unsupervised algorithms and correlations of clinical characteristics between subgroups were analyzed. Next, The CIBERSORT algorithm was used to evaluate the proportion of immune cell infiltration in different OM subgroups. Weighted gene co-expression analysis (WGCNA) was used to identify different gene modules and explore the relationship with clinical characteristics, and further annotated OM subgroups and gene modules by the Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis.

Results
Two subgroups with excellent consistency were identified in this study, subgroup and hospital length of stay were independent predictors of OM. Compared with subgroup I, OM patients in subgroup II had longer hospital length of stay and more severe disease. Meanwhile, the infiltration proportions of monocytes and macrophages M0 were higher in patients of OM subgroup II. Finally, combined with the characteristics of the KEGG enrichment modules, the expression of osteoclast differentiation-related genes such as CTSK was upregulated in OM subgroup II, which may be closely associated with more severe OM patients.

Conclusion
The current study showed that OM subgroup II had longer hospital length of stay and more severe disease, the osteoclast differentiation pathway and the main target CTSK contribute to our deeper understanding for the molecular mechanisms associated with S. aureus infection-induced OM, and the construction of molecular subgroups suggested the necessity for different subgroups of patients to receive individualized treatment.
Dietary diosgenin transcriptionally down-regulated intestinal NPC1L1 expression to prevent cholesterol gallstone formation in mice
Authors: Shen Weiyi , Shao Wentao , Wang Qihan , Wang Bo , Zhao Gang , Gu Aihua , Jiang Zhaoyan , Hu Hai 
Published: June 2023

Abstract
Background
Cholesterol gallstone disease is a common disease. Reducing cholesterol burden is important to prevent/treat gallstone. In this study, we investigated the application of diosgenin (DG) to prevent the formation of gallstone in mice.

Methods
Adult male C57BL/6J mice were fed with the lithogenic diet (LD) only or LD supplemented with DG or ezetimibe for 8 weeks. Incidences of gallstone formation were documented. Intestine and liver tissues were collected to measure the lipid contents and expression of genes in cholesterol metabolism. Caco2 cells were treated with DG to monitor the regulation on cholesterol absorption and the transcriptional regulation of Npc1l1 gene. Changes of gut microbiota by DG was analyzed. Intraperitoneal injection of LPS on mice was performed to verify its effects on STAT3 activation and Npc1l1 expression in the small intestine.

Results
LD led to 100% formation of gallstones in mice. In comparison, dietary DG or ezetimibe supplementary completely prevents gallstones formation. DG inhibited intestinal cholesterol absorption in mice as well as in Caco2 cells by down-regulation of Npc1l1 expression. DG could directly inhibit phosphorylation of STAT3 and its transcriptional regulation of Npc1l1 expression. Furthermore, DG could modulate gut microbiota profiles and LPS mediated STAT3 activation and Npc1l1 expression.

Conclusion
Our results demonstrated that dietary DG could inhibit intestinal cholesterol absorption through decreasing NPC1L1 expression to prevent cholesterol gallstone formation.
WEE1 and PARP-1 play critical roles in myelodysplastic syndrome and acute myeloid leukemia treatment
Authors: Okabe Seiichi , Tanaka Yuko , Moriyama Mitsuru , Gotoh Akihiko 
Published: June 2023

Abstract
Background
Myelodysplastic syndrome (MDS) is a clonal bone marrow disorder defined by cytopenia and is associated with an increased risk of transformation to acute myeloid leukemia (AML). The outcome of MDS is poor, so alternative therapeutic approaches are needed to improve survival. The inhibition of the DNA damage response pathway, including poly (ADP-ribose) polymerase-1 (PARP-1), has been approved to treat several cancers. In addition, WEE1, a nuclear kinase, is overexpressed in many cancers. Therefore, a WEE1 inhibitor combined with a PARP-1 inhibitor could inhibit the proliferation of MDS and AML.

Methods
We analyzed whether WEE1 was regulated in the progression of MDS and AML. We also evaluated the efficacy of MK-1775 (WEE1 inhibitor) and talazoparib (PARP-1 inhibitor).

Results
PARP-1 expression was higher in the AML cells than in the MDS cells. However, WEE1 expression remained unchanged. MK-1775 or talazoparib alone inhibited MDS and AML cells after 72 h, and cellular cytotoxicity and caspase 3/7 activity were increased. The combined use of MK-1775 and talazoparib produced superior efficacy than either drug alone and SKM-1 colony formation was reduced. Significant cell populations in the sub-G1 phase were found in the cell-cycle analyses. Additionally, γ-H2AX expression and caspase 3 activity were increased. The combined treatment also changed the mitochondrial membrane potential.

Conclusions
The combination of a WEE1 inhibitor and PARP-1 inhibitor had enhanced efficacy and is proposed as a new therapeutic option for patients with MDS or AML. Our findings have clinical implications for a potential novel therapeutic strategy for MDS and AML patients.
Wildfire smoke exposure and early childhood respiratory health: a study of prescription claims data
Authors: Dhingra Radhika , Keeler Corinna , Staley Brooke S. , Jardel Hanna V. , Ward-Caviness Cavin , Rebuli Meghan E. , Xi Yuzhi , Rappazzo Kristen , Hernandez Michelle , Chelminski Ann N. , Jaspers Ilona , Rappold Ana G. 
Published: June 2023

AbstractWildfire smoke is associated with short-term respiratory outcomes including asthma exacerbation in children. As investigations into developmental wildfire smoke exposure on children’s longer-term respiratory health are sparse, we investigated associations between developmental wildfire smoke exposure and first use of respiratory medications. Prescription claims from IBM MarketScan Commercial Claims and Encounters database were linked with wildfire smoke plume data from NASA satellites based on Metropolitan Statistical Area (MSA). A retrospective cohort of live infants (2010–2016) born into MSAs in six western states (U.S.A.), having prescription insurance, and whose birthdate was estimable from claims data was constructed (N = 184,703); of these, gestational age was estimated for 113,154 infants. The residential MSA, gestational age, and birthdate were used to estimate average weekly smoke exposure days (smoke-day) for each developmental period: three trimesters, and two sequential 12-week periods post-birth. Medications treating respiratory tract inflammation were classified using active ingredient and mode of administration into three categories:: 'upper respiratory', 'lower respiratory', 'systemic anti-inflammatory'. To evaluate associations between wildfire smoke exposure and medication usage, Cox models associating smoke-days with first observed prescription of each medication category were adjusted for infant sex, birth-season, and birthyear with a random intercept for MSA. Smoke exposure during postnatal periods was associated with earlier first use of upper respiratory medications (1–12 weeks: hazard ratio (HR) = 1.094 per 1-day increase in average weekly smoke-day, 95%CI: (1.005,1.191); 13–24 weeks: HR = 1.108, 95%CI: (1.016,1.209)). Protective associations were observed during gestational windows for both lower respiratory and systemic anti-inflammatory medications; it is possible that these associations may be a consequence of live-birth bias. These findings suggest wildfire smoke exposure during early postnatal developmental periods impact subsequent early life respiratory health.
Universal chromatin state annotation of the mouse genome
Authors: Vu Ha , Ernst Jason 
Published: June 2023

Abstract
A large-scale application of the “stacked modeling” approach for chromatin state discovery previously provides a single “universal” chromatin state annotation of the human genome based jointly on data from many cell and tissue types. Here, we produce an analogous chromatin state annotation for mouse based on 901 datasets assaying 14 chromatin marks in 26 cell or tissue types. To characterize each chromatin state, we relate the states to external annotations and compare them to analogously defined human states. We expect the universal chromatin state annotation for mouse to be a useful resource for studying this key model organism’s genome.
Moving beyond ontological (worldview) supremacy: Indigenous insights and a recovery guide for settler-colonial scientists
Authors: Hird Coen , David-Chavez Dominique M. , Gion Shanny Spang , van Uitregt Vincent 
Published: June 2023

ABSTRACT
Greater engagement and representation of Indigenous voices, knowledges and worldviews in the biological sciences is growing globally through efforts to bring more Indigenous academics into scientific research and teaching institutions. Although the intentions of such efforts may be admirable, these spaces often become sites of great personal tension for the Indigenous scholars who must ‘bridge’ or ‘facilitate’ a dialogue between Indigenous and settler-colonial (predominantly Western) knowledge traditions and worldviews. We are a small collective of early career Indigenous scholars from Australia, the United States and Aotearoa New Zealand, and we have gained insights into this situation through the unique experiential learning afforded by navigating such tensions. Here, we discuss tensions that bear remarkable similarities across geographies, cultures and settler-colonial contexts. In doing so, we aim to support other Indigenous scientists and scholars navigating settler-colonial and Western research institutions, while offering guidance, suggestions and reflections for the scientific community to allow the development of more nuanced strategies to support Indigenous academics than simply increasing Indigenous representation. We imagine transformed, innovative research and teaching agendas where Indigenous knowledges can thrive, and Indigenous scientists can apply themselves with mutual and balanced respect and reciprocity.
Working Paper 44: Perceptions of corruption and anti-corruption efforts in Bulgaria
Authors: Bailer Stefanie , Thürk Maria 
Published: June 2023

The Working Paper presents the results of a nationwide survey of 1,209 individuals in Bulgaria, conducted in February to early March 2023.
The survey examined how corruption is perceived in Bulgaria by different types of respondent, and what kind of behaviour is considered acceptable.
It also looked at respondents’ perceptions of anti-corruption efforts and under which circumstances they would be more likely to report corruption to the authorities.
Microsensors in the retention treatment of orthodontic patients - literature review
Authors: Jasińska Joanna , Łazicka Patrycja , Jakubowska Eliza , Miziniak Marta , Karolak Aleksandra 
Published: June 2023

Introduction
Removable retainers have been successfully used for many years as an independent or additional form of retention in patients after orthodontic treatment. Unfortunately, it has been proven that most patients do not follow the orthodontist's recommendations and do not use the braces for the number of hours necessary to stabilize the treatment effects. It has therefore become necessary to look for solutions and devices that are able to objectively record the actual time of retainers use.
Aim of the study
The aim of the study was to assess the usefulness of electronic microsensors in monitoring wear time of removable retainers.
Materials and methods
A search was conducted using PubMed, Google Scholar, ResearchGate databases. Articles were searched in English using the following key words: “microsensors”, “retention”, “orthodontic”, “TheraMon”, “Smart Retainer”.
Results
The results of the literature review show that microsensors embedded in the elements of removable retainers are a valuable tool for monitoring the time of their use. Awareness of the presence of electronic sensors increases patient discipline. In addition, they provide data that, apart from objectively documenting the course of retention treatment, may be used in the future to determine optimal retention protocols.
Conclusions
The retention stage is an indispensable element of orthodontic treatment. The use of microsensors built into removable retainers can help to improve the stability of treatment results. However, further research and work are needed to improve the performance and reliability of this type of electronic sensors.
Analisis Risiko Ergonomi Pada Petani Karet Di Desa Beringin Jaya Way Tuba Kabupaten Way Kanan, Lampung
Authors: Wibawa Muhammad Gede , ZulArdi Subhan 
Published: June 2023

Background: Some methods for evaluating work postures include RULA, REBA, OWAS and QEC. Beringin Jaya Village there are 387 families with a population of 1,255 people. 85% of the population work as rubber farmers. Some rubber farmers in Beringin Jaya Village, farmers experience complaints of pain in the neck and back. The purpose of this study is to analyze the ergonomic risk of rubber farmers in the Beringin Jaya Village, Way Tuba, Way Kanan Regency, Lampung
Methods: The design of this study was descriptive qualitative with a case study design and using the REBA survey tool. The research subjects in this study were rubber farmers in Beringin Jaya Village, Way Tuba, Way Kanan Regency, Lampung. The sampling technique in this study is snow ball sampling technique. The type of data in this study is primary data, obtained through observation, measurement and interviews. data analysis with Miles and Huberman modes.
Results: the level of ergonomics risk in the treatment process scores 11+, the harvesting process scores 11+ at the tapping stage, the rubber gum scavenging stage scores 11+, the sales process gets a final score of 9.
Conclusion: the level of ergonomic risk in all the work processes of farmers in Beringin Jaya Village, Way Tuba, Way Kanan district, Lampung Province get high to very high risk levels.