bluuebunny/crossref_metadata_2025_split huggingface dataset:
Arabidopsis stromal carbonic anhydrases exhibit non‐overlapping roles in photosynthetic efficiency and development
Authors: Sharma Naveen , Froehlich John E. , Rillema Rees , Raba Daniel A. , Chambers Taylor , Kerfeld Cheryl A. , Kramer David M. , Walker Berkley , Brandizzi Federica 
Published: April 2023

SUMMARYCarbonic anhydrases (CAs) are ubiquitous enzymes that accelerate the reversible conversion of CO2 to HCO3−. The Arabidopsis genome encodes members of the α‐, β‐ and γ‐CA families, and it has been hypothesized that βCA activity has a role in photosynthesis. In this work, we tested this hypothesis by characterizing the two plastidial βCAs, βCA1 and βCA5, in physiological conditions of growth. We conclusively established that both proteins are localized in the chloroplast stroma and that the loss of βCA5 induced the expression of βCA1, supporting the existence of regulatory mechanisms to control the expression of stromal βCAs. We also established that βCA1 and βCA5 have markedly different enzymatic kinetics and physiological relevance. Specifically, we found that βCA5 had a first‐order rate constant ~10‐fold lower than βCA1, and that the loss of βCA5 is detrimental to growth and could be rescued by high CO2. Furthermore, we established that, while a βCA1 mutation showed near wild‐type growth and no significant impact on photosynthetic efficiency, the loss of βCA5 markedly disrupted photosynthetic efficiency and light‐harvesting capacity at ambient CO2. Therefore, we conclude that in physiological autotrophic growth, the loss of the more highly expressed βCA1 does not compensate for the loss of a less active βCA5, which in turn is involved in growth and photosynthesis at ambient CO2 levels. These results lend support to the hypothesis that, in Arabidopsis,βCAs have non‐overlapping roles in photosynthesis and identify a critical activity of stromal βCA5 and a dispensable role for βCA1.
Antibacterial PES/Vancomycin ultrafiltration membranes with enhanced water flux and low BSA rejection
Authors: Abid Zubia , Abbas M. Asad , Janjua Hussnain A. , Ahmed Naveed , Ahmad Nasir M. 
Published: April 2023

AbstractPolyethersulfone (PES) blended with glycopeptide Vancomycin was fabricated via phase inversion method. The fabricated membranes were characterized by scanning electron microscopy (SEM), fourier transform infrared spectroscopy (FTIR), contact angle (CA) and optical profilometry. The PES/Vancomycin membranes showed a notable increase in hydrophilicity, porosity, pore size, antibacterial activity and water flux in comparison with pristine membranes. The changes are due to the hydrophilic nature of Vancomycin molecules and ability to form hydrogen bond quickly. The surface hydrophilicity of PES/Vancomycin membranes decreased up to 48° with increase in surface roughness (Ra), that is, 0.347 μm, thus increasing the water flux of PES/Vancomycin membranes up to 340 L/m2. h. The bovine serum albumin (BSA) rejection decreases, that is, pristine 70.9% to PES/Vancomycin 26% and BSA flux increases at constant ionic strength (0.1) and above the isoelectric point (IEP) of the BSA. Under these conditions, electrostatic interactions are expected to be minimum between protein and the membrane surfaces.
Active learning BSM parameter spaces
Authors: Goodsell Mark D. , Joury Ari 
Published: April 2023

AbstractActive learning (AL) has interesting features for parameter scans of new models. We show on a variety of models that AL scans bring large efficiency gains to the traditionally tedious work of finding boundaries for BSM models. In the MSSM, this approach produces more accurate bounds. In light of our prior publication, we further refine the exploration of the parameter space of the SMSQQ model, and update the maximum mass of a dark matter singlet to 48.4 TeV. Finally we show that this technique is especially useful in more complex models like the MDGSSM.
Current Approaches to Serving Catchment Areas in Cancer Centers: Insights from the Big Ten Cancer Research Consortium Population Science Working Group
Authors: Manne Sharon L. , Knott Cheryl L. , Berger Ann , Champion Victoria L. , Chrischilles Elizabeth , Fitzgibbon Marian L. , Kinney Anita Y. , Lengerich Eugene J. , Nash Sarah H. , Simon Melissa A. , Trentham-Dietz Amy , Paskett Electra D. 
Published: April 2023

AbstractIn 2021, the NCI issued updated guidance clarifying the mission and organizational structure for Community Outreach and Engagement (COE) for Cancer Center Support Grants. These guidelines outlined how cancer centers should address the cancer burden of the catchment area (CA) and define how COE would partner with the community to inform cancer research and implement programs to reduce the cancer burden. In this paper, the Common Elements Committee of the Population Science Working Group in the Big Ten Cancer Research Consortium describes their respective approaches to implementing these guidelines. We discuss our definitions and rationales for each CA, data sources used, and our approach to assessing the impact of COE efforts on the burden of cancer in our respective CA. Importantly, we describe methods of translating unmet CA needs into our cancer-relevant outreach activities, and cancer research addressing the needs of respective CAs. Implementing these new guidelines is a challenge, and we hope that sharing approaches and experiences will foster cross-center collaborations that may more effectively reduce the burden of cancer in the US and meet the mission of the NCI's Cancer Center Program.
Supplementary Data: Figure S1-3 and Table S1-S5 from Preclinical Antitumor Activity and Biodistribution of a Novel Anti-GCC Antibody–Drug Conjugate in Patient-derived Xenografts
Authors: Abu-Yousif Adnan O. , Cvet Donna , Gallery Melissa , Bannerman Bret M. , Ganno Michelle L. , Smith Michael D. , Lai Katharine C. , Keating Thomas A. , Stringer Bradley , Kamali Afrand , Eng Kurt , Koseoglu Secil , Zhu Andy , Xia Cindy Q. , Landen Melissa Saylor , Borland Maria , Robertson Robbie , Bolleddula Jayaprakasam , Qian Mark G. , Fretland Jennifer , Veiby O. Petter 
Published: April 2023

<p>Supplemental Figure 1: heavy and light chain sequences for 5F9, the human IgG1 monoclonal antibody (mAb) targeting the extracellular domain of GCC as described previously Supplemental Figure 2: Characterization of TAK-164 and chKTI-DGN549 by A) SDS PAGE gel and B) by Liquid chromatography-mass spectrometry (LC-MS) Supplemental Figure 3: In vivo activity of TAK-164 in PHTXM-46C Supplemental Table 1: Growth rate inhibition following treatment with TAK-164 and B)	chKTI-ADC in PHTX and cell derived xenograft models Supplemental Table 2: Growth Rate Inhibition and associated statistics for HEK293 GCC#2 Supplemental Table 3: Growth Rate Inhibition and associated statistics for PHTX-09C Supplemental Table 4: Growth Rate Inhibition and associated statistics for PHTX-17C Supplemental Table 5: Growth Rate Inhibition and associated statistics for PHTX-11C</p>
Supplementary Fig S8 from Heme Sequestration as an Effective Strategy for the Suppression of Tumor Growth and Progression
Authors: Wang Tianyuan , Ashrafi Adnin , Konduri Purna Chaitanya , Ghosh Poorva , Dey Sanchareeka , Modareszadeh Parsa , Salamat Narges , Alemi Parinaz Sadat , Berisha Eranda , Zhang Li 
Published: April 2023

<p>Figure S8. A, Representative montage and 10X IHC images of H1299 NSCLC tumor tissue sections and graphs showing the levels of ï�¢-actin in control and HeSP2- or HeSP2del-treated tumors. B, Representative montage and 10X IHC images of H1299 NSCLC tumor tissue sections and graphs showing the levels of GAPDHï€ in control and HeSP2- or HeSP2del-treated tumors. C, Representative montage and 10X IHC images of H1299 NSCLC tumor tissue sections and graphs showing the levels of TOM20 in control and HeSP2- or HeSP2del-treated tumors. D, Representative montage and 10X IHC images of H1299 NSCLC tumor tissue sections and graphs showing the levels of VDAC in control and HeSP2- or HeSP2del-treated tumors. Protein levels were quantified. Data were plotted as mean {plus minus} SEM. E, Representative 10X IHC images of H1299 NSCLC tumor tissue sections showing the levels of VEGFR1 in control and HeSP-treated tumors. F, Representative montage and 10X IHC images of H1299 NSCLC tumor tissue sections and graphs showing the levels of VEGFA in control and HeSP-treated tumors. Shown IHC figures are montages or 10X images of control and HeSP-treated tumor tissue sections stained with DAPI or antibodies against the indicated protein. The white rectangles in DAPI images denote the regions shown in 10X images. Scale bar: montage, 1 mm; 10X, 200 Î¼m. Protein levels were quantified, and data were plotted as mean {plus minus} SEM. ***, P < 0.005. G, Representative 40X IHC images of H1299 NSCLC tumor tissue sections showing CD31 staining in control and HeSP-treated tumors. H, Representative 40X IHC images of H1299 NSCLC tumor tissue sections showing CD34 staining in control and HeSP-treated tumors. Scale bar, 50Âµm.</p>
Data from Next Generation Sequencing of Prostate Cancer from a Patient Identifies a Deficiency of Methylthioadenosine Phosphorylase, an Exploitable Tumor Target
Authors: Collins Colin C. , Volik Stanislav V. , Lapuk Anna V. , Wang Yuwei , Gout Peter W. , Wu Chunxiao , Xue Hui , Cheng Hongwei , Haegert Anne , Bell Robert H. , Brahmbhatt Sonal , Anderson Shawn , Fazli Ladan , Hurtado-Coll Antonio , Rubin Mark A. , Demichelis Francesca , Beltran Himisha , Hirst Martin , Marra Marco , Maher Christopher A. , Chinnaiyan Arul M. , Gleave Martin , Bertino Joseph R. , Lubin Martin , Wang Yuzhuo 
Published: April 2023

<div>Abstract<p>Castrate-resistant prostate cancer (CRPC) and neuroendocrine carcinoma of the prostate are invariably fatal diseases for which only palliative therapies exist. As part of a prostate tumor sequencing program, a patient tumor was analyzed using Illumina genome sequencing and a matched renal capsule tumor xenograft was generated. Both tumor and xenograft had a homozygous 9p21 deletion spanning the <i>MTAP</i>, <i>CDKN2</i>, and <i>ARF</i> genes. It is rare for this deletion to occur in primary prostate tumors, yet approximately 10% express decreased levels of methylthioadenosine phosphorylase (MTAP) mRNA. Decreased <i>MTAP</i> expression is a prognosticator for poor outcome. Moreover, it seems that this deletion is more common in CRPC than in primary prostate cancer. We show for the first time that treatment with methylthioadenosine and high dose 6-thioguanine causes marked inhibition of a patient-derived neuroendocrine xenograft growth while protecting the host from 6-thioguanine toxicity. This therapeutic approach can be applied to other MTAP-deficient human cancers as deletion or hypermethylation of the <i>MTAP</i> gene occurs in a broad spectrum of tumors at high frequency. The combination of genome sequencing and patient-derived xenografts can identify candidate therapeutic agents and evaluate them for personalized oncology. <i>Mol Cancer Ther; 11(3); 775–83. ©2012 AACR</i>.</p></div>
Desenvolvimento do sistema nociceptivo e supressor da dor
Authors: Okada Massako , Teixeira Manoel Jacobsen , Tengan Sérgio K. , Moraes Lygia Oliveira de 
Published: April 2023

Há várias evidências de que nas fases precoces em desenvolvimento fetal ocorra a deflagração de mecanismos deacarretam a estruturação anatômica e funcional do sistema nervoso sensitivo. Após o nascimento muitas modificações ocorrem durante os primeiros meses de vida extra-uterina. O conhecimento destas condições é necessário para melhorar a compreensão da dor e as atitudes analgésicas.
miR-34a-5p as molecular hub of pathomechanisms in Huntington’s disease
Authors: Hart Martin , Diener Caroline , Lunkes Laetitia , Rheinheimer Stefanie , Krammes Lena , Keller Andreas , Meese Eckart 
Published: April 2023

Abstract
Background
Although a pivotal role of microRNA (miRNA, miR) in the pathogenesis of Huntington’s disease (HD) is increasingly recognized, the molecular functions of miRNAs in the pathomechanisms of HD await further elucidation. One of the miRNAs that have been associated with HD is miR-34a-5p, which was deregulated in the mouse R6/2 model and in human HD brain tissues.

Methods
The aim of our study was to demonstrate interactions between miR-34a-5p and HD associated genes. By computational means we predicted 12 801 potential target genes of miR-34a-5p. An in-silico pathway analysis revealed 22 potential miR-34a-5p target genes in the KEGG (Kyoto Encyclopedia of Genes and Genomes) pathway “Huntington’s disease”.

Results
Using our high-throughput miRNA interaction reporter assay (HiTmIR) we identified NDUFA9, TAF4B, NRF1, POLR2J2, DNALI1, HIP1, TGM2 and POLR2G as direct miR-34a-5p target genes. Direct binding of miR-34a-5p to target sites in the 3’UTRs of TAF4B, NDUFA9, HIP1 and NRF1 was verified by a mutagenesis HiTmIR assay and by determining endogenous protein levels for HIP1 and NDUFA9. STRING (Search Tool for the Retrieval of Interacting Genes/Proteins) analysis identified protein–protein interaction networks associated with HD like “Glutamine Receptor Signaling Pathway” and “Calcium Ion Transmembrane Import Into Cytosol”.

Conclusion
Our study demonstrates multiple interactions between miR-34a-5p and HD associated target genes and thereby lays the ground for future therapeutic interventions using this miRNA.
An optimized workflow for MS-based quantitative proteomics of challenging clinical bronchoalveolar lavage fluid (BALF) samples
Authors: Weise Danielle O. , Kruk Monica E. , Higgins LeeAnn , Markowski Todd W. , Jagtap Pratik D. , Mehta Subina , Mickelson Alan , Parker Laurie L. , Wendt Christine H. , Griffin Timothy J. 
Published: April 2023

AbstractBackgroundClinical bronchoalveolar lavage fluid (BALF) samples are rich in biomolecules, including proteins, and useful for molecular studies of lung health and disease. However, mass spectrometry (MS)-based proteomic analysis of BALF is challenged by the dynamic range of protein abundance, and potential for interfering contaminants. A robust, MS-based proteomics compatible sample preparation workflow for BALF samples, including those of small and large volume, would be useful for many researchers.ResultsWe have developed a workflow that combines high abundance protein depletion, protein trapping, clean-up, and in-situ tryptic digestion, that is compatible with either qualitative or quantitative MS-based proteomic analysis. The workflow includes a value-added collection of endogenous peptides for peptidomic analysis of BALF samples, if desired, as well as amenability to offline semi-preparative or microscale fractionation of complex peptide mixtures prior to LC–MS/MS analysis, for increased depth of analysis. We demonstrate the effectiveness of this workflow on BALF samples collected from COPD patients, including for smaller sample volumes of 1–5 mL that are commonly available from the clinic. We also demonstrate the repeatability of the workflow as an indicator of its utility for quantitative proteomic studies.ConclusionsOverall, our described workflow consistently provided high quality proteins and tryptic peptides for MS analysis. It should enable researchers to apply MS-based proteomics to a wide-variety of studies focused on BALF clinical specimens.
High SARS-CoV-2 seroprevalence among street adolescents in Lomé, Togo, 2021
Authors: Sadio Arnold Junior , Ferré Valentine Marie , Konu Rodion Yao , Dagnra Anoumou Claver , Descamps Diane , Ekouevi Didier Koumavi , Charpentier Charlotte 
Published: April 2023

Abstract
Background
There is almost no data on the circulation of SARS-CoV-2 among street adolescents. We conducted a study to document the immunization status of street adolescents in Togo against different variants of SARS-CoV-2.

Methods
A cross-sectional study was carried out in 2021 in Lomé, the city with the highest number of COVID 19 cases in Togo (60%). Adolescents aged 13- and 19 years old living on the street were eligible for inclusion. A standardized questionnaire was administered face-to-face to adolescents. A sample of blood was taken and aliquots of plasma were transported to the virology laboratory of the Hôpital Bichat-Claude Bernard (Paris, France). SARS-CoV-2 anti-S and anti-N IgG were measured using chemiluminescent microparticle immunoassay. A quantitative miniaturized and parallel-arranged ELISA assay was used to detect IgG antibodies specifically directed against the different SARS-CoV-2 Variants of Concern (VOC).

Results
A total of 299 street adolescents (5.2% female), median age 15 years, interquartile range (14-17 years), were included in this study. The prevalence of SARS-CoV-2 infection was 63.5% (95%CI: 57.8–69.0). Specific-IgG against the ancestral Wuhan strain was developed by 92.0% of subjects. The proportion of patients being immunized against each VOC was 86.8%, 51.1%, 56.3%, 60.0, and 30.5% for the Alpha, Beta, Gamma, Delta, and Omicron VOCs, respectively.

Conclusion
This study showed a very high prevalence with approximately 2/3 of Togolese street adolescents having antibodies to SARS-CoV-2 due to a previous infection. These results confirm an under-reporting of COVID-19 cases in Togo, questioning the hypothesis of low virus circulation in Togo and even in Africa.
Clinical significance of nonerythrocytic spectrin Beta 1 (SPTBN1) in human kidney renal clear cell carcinoma and uveal melanoma: a study based on Pan-Cancer Analysis
Authors: Tang Wenting , Shao Qiong , He Zhanwen , Zhang Xu , Li Xiaojuan , Wu Ruohao 
Published: April 2023

Abstract
Background
Nonerythrocytic spectrin beta 1 (SPTBN1) is an important cytoskeletal protein that involves in normal cell growth and development via regulating TGFβ/Smad signaling pathway, and is aberrantly expressed in various cancer types. But, the exact role of SPTBN1 in pan-cancer is still unclear. This report aimed to display expression patterns and prognostic landscapes of SPTBN1 in human cancers, and further assess its prognostic/therapeutic value and immunological role in kidney renal carcinoma (KIRC) and uveal melanoma (UVM).

Methods
We firstly analyzed expression patterns and prognostic landscapes of SPTBN1 in human cancers using various databases and web-based tools. The relationships between SPTBN1 expression and survival/tumor immunity in KIRC and UVM were further investigated via R packages and TIMER 2.0 platform. The therapeutic roles of SPTBN1 in KIRC and UVM were also explored via R software. Following this, the prognostic value and cancer immunological role of SPTBN1 in KIRC and UVM were validated in our cancer patients and GEO database.

Results
Overall, cancer tissue had a lower expression level of SPTBN1 frequently in pan-cancer, compared with those in adjacent nontumor one. SPTBN1 expression often showed a different effect on survival in pan-cancer; upregulation of SPTBN1 was protective to the survival of KIRC individuals, which was contrary from what was found in UVM patients. In KIRC, there were significant negative associations between SPTBN1 expression and pro-tumor immune cell infiltration, including Treg cell, Th2 cell, monocyte and M2-macrophage, and expression of immune modulator genes, such as tumor necrosis factor superfamily member 9 (TNFSF9); while, in UVM, these correlations exhibited opposite patterns. The following survival and expression correlation analysis in our cancer cohorts and GEO database confirmed these previous findings. Moreover, we also found that SPTBN1 was potentially involved in the resistance of immunotherapy in KIRC, and the enhance of anti-cancer targeted treatment in UVM.

Conclusions
The current study presented compelling evidence that SPTBN1 might be a novel prognostic and therapy-related biomarker in KIRC and UVM, shedding new light on anti-cancer strategy.
A meta-analysis of internet-based cognitive behavioral therapy for military and veteran populations
Authors: Liu Jenny JW , Ein Natalie , Forchuk Callista , Wanklyn Sonya G. , Ragu Suriya , Saroya Samdarsh , Nazarov Anthony , Richardson J. Don 
Published: April 2023

Abstract
Background
Military and veteran populations are unique in their trauma exposures, rates of mental illness and comorbidities, and response to treatments. While reviews have suggested that internet-based Cognitive Behavioral Therapy (iCBT) can be useful for treating mental health conditions, the extent to which they may be appropriate for military and veteran populations remain unclear. The goals of the current meta-analysis are to: (1) substantiate the effects of iCBT for military and veteran populations, (2) evaluate its effectiveness compared to control conditions, and (3) examine potential factors that may influence their effectiveness.

Methods
This review was completed following the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) reporting and Cochrane review guidelines. The literature search was conducted using PsycInfo, Medline, Embase, and Proquest Dissertation & Theses on June 4, 2021 with no date restriction. Inclusion criteria included studies that: (1) were restricted to adult military or veteran populations, (2) incorporated iCBT as the primary treatment, and (3) evaluated mental health outcomes. Exclusion criteria included: (1) literature reviews, (2) qualitative studies, (3) study protocols, (4) studies that did not include a clinical/analogue population, and (5) studies with no measure of change on outcome variables. Two independent screeners reviewed studies for eligibility. Data was pooled and analyzed using random-effects and mixed-effects models. Study data information were extracted as the main outcomes, including study condition, sample size, and pre- and post-treatment means, standard deviations for all assessed outcomes, and target outcome. Predictor information were also extracted, and included demographics information, the types of outcomes measured, concurrent treatment, dropout rate, format, length, and delivery of intervention.

Results
A total of 20 studies and 91 samples of data were included in the meta-analysis. The pooled effect size showed a small but meaningful effect for iCBT, g = 0.54, SE = 0.04, 95% CI (0.45, 0.62), Z = 12.32, p < .001. These effects were heterogenous across samples, (I2 = 87.96), Q(90) = 747.62, p < .001. Predictor analyses found length of intervention and concurrent treatment to influence study variance within sampled studies, p < .05. Evaluation of iCBT on primary outcomes indicated a small but meaningful effect for PTSD and depression, while effects of iCBT on secondary outcomes found similar results with depression, p < .001.

Conclusions
Findings from the meta-analysis lend support for the use of iCBT with military and veteran populations. Conditions under which iCBT may be optimized are discussed.