bluuebunny/crossref_metadata_2025_split huggingface dataset:
Association Between Serum Levels of Anti-Heat Shock Protein-27 Antibody and Liver Cell Injury in Chronic Hepatitis B
Authors: Yousefli Zahra , Meshkat Zahra , Mobarhan Majid Ghayour , Hosseini Seyed Mousalreza , Tavalaie Shima , Kazemianfar Ehsan , Soltanian Hossein , Aminzadeh Afarin , Ghasemi Amirhosein , Kashmari Maryam , Nasiraee Mohammadreza , Meshkat Mojtaba , Jarrahi Lida , Gholoobi Aida 
Published: December 2022

Abstract
Background
Heat Shock Protein 27 (HSP27), an anti-HBV factor, exists in the intracellular and extracellular spaces. Serum HSP27 (sHSP27) is an inflammatory modulator and is associated with elevated pro-inflammatory cytokines and with a higher likelihood of hepatocellular carcinoma. SHSP27 results in natural antibody production (anti-HSP27-Ab) that is more stable and easily detectable compared to sHSP27. We aimed to investigate any potential association between anti-HSP27-Ab level and chronic hepatitis B (CHB) progression and inflammation indicated by liver cell injury and HBV replication.
Methods
This cross-sectional study was conducted on 91 patients with CHB and 92 individuals without CHB. Following demographic data collection, anti-HSP27-Ab, serum lipids including total cholesterol, triglyceride, LDL-C, HDL-C, and aminotransferase levels were measured using enzymatic assays in participants’ serum samples. HBV DNA was also measured by quantitative PCR in CHB patients.
Results
Bivariate and multivariate analyses showed a significantly higher mean level of anti-HSP27-Ab in CHB than in healthy individuals (0.304 vs. 0.256AU/ml, P-value = 0.015). These levels held significant differences in the CHB subgroups of male patients, at the age of 50 years and above, non-smokers, patients with elevated aminotransferase levels, and hypotriglyceridemia (P-value < 0.05). However, no difference was found between the antibody levels and HBV DNA copies (P-value > 0.05).
Conclusion
This study provides evidence that anti-HSP27 antibody levels can reflect the degree of liver necrosis indicated by aminotransferase levels. Regarding the higher incidence rate of HBV-associated complications in 50 to 60-year-old men, monitoring the antibody can be beneficial in managing this group of CHB patients, which deserves further investigation.
Urgent care-seeking and injury severity for intimate partner violence during COVID-19: a Canadian retrospective chart review
Authors: Ross Alison N. , Duchesne Emma , Lewis Jane , Norman Patrick A. , Bartels Susan A. , Walker Melanie , Rocca Nicole 
Published: December 2022

Abstract

Background Early reports raised alarms that intimate partner violence (IPV) increased during the COVID-19 pandemic, but initial studies showed that visits to emergency departments (EDs) decreased. This study assessed the impact of the prolonged pandemic and its associated restrictions on both rates of urgent care-seeking and injury severity for IPV. Methods Data from the Kingston Health Sciences Centre’s (KHSC) ED were utilized to compare IPV presentations during ‘Pre-COVID’ (December 17, 2018 – March 16, 2020) and ‘COVID’ (March 17, 2020 – June 16, 2021), as well as three periods of heightened local restrictions: ‘Lockdown-1’ (March 17 – June 12, 2020), ‘Lockdown-2’ (December 26, 2020 – February 10, 2021) and ‘Lockdown-3’ (April 8 – June 2, 2021). The primary outcomes were incidence rate of IPV visits and injury severity, which was assessed using theClinical Injury Extent Score (CIES)andInjury Severity Score (ISS).Results A total of 128 individuals were included. This sample had mean age of 34 years, was comprised of mostly women (97%), and represented a variety of intimate relationship types. Some individuals presented multiple times, resulting in a total of 139 acute IPV presentations. The frequency of IPV visits during COVID was similar to the Pre-COVID time period (67 vs. 72; p = 0.67). Incidence rate was 13% higher during COVID, though this difference was non-significant (6.66 vs. 5.90; p = 0.47). IPV visit frequency varied across lockdown periods (11 in Lockdown-1, 12 in Lockdown-2 and 6 in Lockdown-3), with the highest incidence rate during Lockdown-2 (12.71). There were more moderate and severe injuries during COVID compared to Pre-COVID, but mean CIES was not statistically significantly different (1.91 vs. 1.69; p = 0.29), nor was mean ISS (11.88 vs. 12.52; p = 0.73). Conclusions During the 15-months following the start of COVID-19, there were small, but non-significant increases in both incidence rate and severity of IPV presentations to the KHSC ED. This may reflect escalation of violence as pandemic restrictions persisted and requires further investigation.
Age-Period-Cohort Analysis of Global Prevalence of Blindness and Vision Loss: Findings From The Global Burden of Disease Study 2019
Authors: Guo Chengyao , Li Yuancun , Huang Yingzi , Jing Liu , Qiu Kunliang , Zhang Mingzhi 
Published: December 2022

Abstract
Objective
To quantify age, period, and cohort effect on the global secular trend of prevalence of blindness and vision impairment (BVI) based on the age-period-cohort (APC) model.
Methods
Data on global BVI were extracted from the Global Burden of Diseases, Injuries, and Risk Factors Study (GBD) 2019 database. Annual percentage change of age-standardized prevalence rate (ASPR) of BVI was estimated by assuming a linear relationship between natural logarithm of ASPR of disease with time. The prevalence of BVI was evaluated from age, period, and cohort effects based on the APC model with intrinsic estimator.
Results
Global prevalence number of BVI was 353.2 million in 1990 and increased to 713.9 million in 2019, but with an ASPR declined at a speed of -0.14% (95% CI: -7.49–7.8%) per year from 1990 to 2019. The APC model showed that the prevalence of BVI increased with age and period but decreased with cohorts. Changes in each cause (age-related macular degeneration, cataract, glaucoma, refractive disorders, near-vision loss, and other vision loss) are consistent in the overall upward or downward trend of the age, period, and cohort effects.
Conclusions
Global prevalence of BVI has significant age, period and cohort effects. The risk of vision impairment increases with age and period, however, it decreases with the cohort. Cost-effective prevention and control should be implemented more in the older population at high risk.
WITHDRAWN: In-silico discovery of novel microtubule inhibitors targeting colchicine binding site; A combined Group-based QSAR approach
Authors: Sahu Sumanta Kumar , Ojha Krishna Kumar 
Published: December 2022

Abstract
Microtubules are a potential target for the design and development of novel anti-mitotic drugs for cancer therapy Focusing on their mechanisms of action, Microtubuletargeting agents are classified into stabilizers and destabilizers, among them destabilizers binding to colchicine binding site domain is an important source of research in recent years. A number of molecules containing indole scaffold have been described as tubulin polymerization inhibitors with the potential to interact with the colchicine binding site. The research is focused on the search for new indole-based colchicine binding site inhibitors, for that fragment-based QSAR utilized for the important interacting site for potent fragment attachment and the designed fragment library screened for the finding of the potent molecule and finally, three molecules screened and validated for their reactivity using DFT and stability using Molecular dynamics simulation, among them m16 showing the potential result with high interaction energy, high molecular reactivity and confirms high stability as compared to others.
A Novel Anoikis-revelant Gene Signature for Prognosis Prediction and Tumor Immune Microenvironment in Lung Adenocarncinoma
Authors: Ma Yong , Li Zhilong , Xue Yanfeng , Zheng Baozhen , Hu Nan , Li Dongbing , Wang Dongliang 
Published: December 2022

Abstract
Background: Anoikis is an apoptotic cell death, which is resulting from the loss of interaction between cells and the extracellular matrix, and has served a prominent role in metastasis. The aim of the present study was to identify an anoikis-revelant genes (ARGs) signature for Lung Adenocarncinoma (LUAD) patients’ prognosis and explore the underlying molecular mechanisms.
Methods: In the training cohort, LUAD patients from The Cancer Genome Atlas (TCGA) were used, and Gene Expression Omnibus (GEO) cohort GSE72094 was used for validation. A total of 508 anoikis-revelant genes downloaded from the GeneCards. Univariate Cox analysis was applied for preliminary screening of anoikis-revelant genes with potential prognostic capacity in the training cohort. These genes were then applied into an overall survival-based LASSO regression model, building a gene signature. The discovered gene signature was then evaluated via Kaplan–Meier (KM), Cox, and ROC analyses in both cohorts. To better explore the functional annotation of the gene signature and the character of tumor microenvironment, the GSEA enrichment and CIBERSORT algorithm were performed.
Results: A thirteen-gene signature was built in the TCGA-LUAD cohort and further validated in GSE72094 cohort, revealing its independent prognosis value in LUAD. Next, the signature's predictive ability for LUAD prognosis was confirmed through ROC analysis. Moreover, analyses of gene enrichment and immune infiltrating detailed exhibited cell adhesion and VEGF pathways related with the thirteen-gene signature, also showing that M0 macrophages, mast cells, dendritic cells and CD4+ memory T cells involved in the prognosis of the thirteen-gene signature.
Conclusions: An inventive anoikis-revelant thirteen-gene signature (ABHD4, CDCP1, CDK1, CENPF, EIF2AK3, FADD, FYN, HGF, OGT, PIK3CG, PPP2CA, RAC1, and XRCC5) was generated through this study. It could accurately predict LUAD prognosis and was related to M0 macrophages, mast cells, dendritic cells, and CD4+ memory T cells.
Knowledge and Practices of the Fourth Year Degree Nursing Students Regarding Tuberculosis Management at a University in Khomas Region, Namibia
Authors: Ntinda Lydia , Kadhila Joseph Galukeni 
Published: December 2022

Abstract
Background: Namibia has one of the highest incidence rates in the world reporting nearly 10 000 cases in 2015, with multi-drug resistants’ TB prevalence of between 3.9 percent for new cases 8.7 percent for previously treated cases. Regardless of Namibia being among the countries that are worst affected by TB, significant improvements have been made in the country in terms of TB diagnosis, treatment and care. Intensified screening, introduction of WHO-recommended rapid molecular testing for TB, Directly Observed Treatment (DOT, Tuberculosis Preventative Therapy (TPT) eligible for PLHIV and others have been adapted to deal with increasing TB incidence.
Methods: The researcher applied a quantitative descriptive research design carried out among fourth year nursing students at the University of Namibia, main campus. Data was collected using self- developed and self-handed printed questionnaires. A simple random sampling method was used to obtain a sample from the population. The population size of the study was 74, however only 69 responses were attainable.
Results: The overall perceived knowledge regarding TB management was generally satisfactory. The findings of this study also revealed that nursing students had fair practices towards the management of TB as some students still practiced bad and unethical practices regarding TB management.
Conclusion: The University of Namibia should also introduce a specific module, preferably from 1st year specifically on basic TB management. Future researches also recommended on topics such as Experiences of nursing students working in TB wards/clinics and the knowledge, attitude and practices of nursing students when caring for TB positive patients.
Retroperitoneal urothelial carcinoma arising after bladder diverticulectomy: a case report
Authors: Ohtsu Akira , Arai Seiji , Fujizuka Yuji , Fukuda Reon , Hori Keisuke , Morimura Yuki , Kawahara Rintaro , Shiraishi Takuya , Ogawa Hiroomi , Miyazawa Yoshiyuki , Nomura Masashi , Sekine Yoshitaka , Koike Hidekazu , Matsui Hiroshi , Suzuki Kazuhiro 
Published: December 2022

Abstract
Background
Urothelial carcinoma arises from the inner urothelial membrane of the renal pelvis, ureter, and bladder and often causes macrohematuria. Here, we report a rare case in which the patient developed non-symptomatic urothelial carcinoma anatomically outside the bladder wall 17 years after bladder diverticulectomy.
Case presentation
An 82-year-old male patient underwent gastrectomy for stomach cancer and hepatectomy for intrahepatic cholangiocarcinoma. Follow-up computed tomography revealed a tumor in the retroperitoneal space, where a bladder diverticulum was removed 17 years earlier. Multiparametric magnetic resonance imaging suggested that the tumor was malignant with rectal invasion. Subsequent computed tomography-guided percutaneous biopsy revealed that the tumor was urothelial carcinoma. The patient underwent two courses of neoadjuvant chemotherapy followed by pelvic exenteration with pelvic lymph node dissection. He is currently receiving adjuvant therapy with an immune checkpoint inhibitor and has had no recurrence for 3 months.
Conclusions
Multiparametric magnetic resonance imaging is a helpful tool for predicting both tumor malignancy and invasion before a pathologically confirmed diagnosis. Although this case is rare, urologists should be aware of the occurrence of urothelial carcinoma after bladder diverticulectomy in cases of incomplete resection of the diverticulum.
Ecological risk assessment of trace elements pollution and human health risk exposure in agricultural soils used for Saffron cultivation
Authors: Taghavi Mahmoud , Darvishiyan Mostafa , Momeni Maryam , Eslami Hadi , Fallahzadeh Reza Ali , Zarei Ahmad 
Published: December 2022

Abstract
Contamination of farmland soils by trace elements has become an international issue concerning food safety and human health risks. In the present research, the concentrations of trace elements including cadmium (Cd), cobalt (Co), chromium (Cr), copper (Cu), manganese (Mn), nickel (Ni), lead (Pb), zinc (Zn) and iron (Fe) in soils of 16 farmlands was determined in Gonabad, Iran. In addition, the human health risks due to exposure to the trace elements from the soils were assessed. Moreover, the soil contamination likelihood was evaluated based on various contamination indices including contamination factor \((\text{C}\text{F}\)), enrichment factor (EF), geo-accumulation index (Igeo), and pollution load index (PLI) calculations. The soil mean concentrations for Cd, Co, Cr, Cu, Mn, Ni, Pb, Zn and Fe ranges as 0.102, 6.968, 22.550, 29.263, 475.281, 34.234, 13.617, 54.482 and 19683.6 mg/kg in farmland soils. The mean concentrations of the trace elements decreased in the order of Fe > Mn > Zn > Ni > Cu > Cr > Pb > Co > As > Cd. Levels of all metals in this study were within the FAO/WHO and Iranian soil standards. The HQ values from investigated elements for adults and children in the studies farms was less than the limit of 1, indicating no health risks for the studied subpopulations. The results of the present research indicated no significant carcinogenic health hazards for both adults and children through ingestion, skin contact and inhalation exposure routes. \(\text{C}\text{F}\) values of for Ni and Zn in 100 % an 6.25 % offarmlands were above 1, showing moderate contamination conditions. EF values of metals in farmlands were recorded as “no enrichment”, “minimal enrichment” and “moderate enrichment” classes. Furthermore, it can be concluded that the all farms were uncontaminated except Ni (moderately contaminated) based on Igeo. PLI showed no contaminated conditions in the entire area. This is an indication that the selected trace elements in the agricultural soils have no appreciable threat to human health.
RETRACTED: Fabrication and characterization of a biodegradable simple, inexpensive cellulose nanofiber (CNF)-based filter for antibiotic removal from wastewater
Authors: Alam M. M. , Islam Md. Johurul , Naher Sumsun , Grattan Ken , Heffernan Claire 
Published: December 2022

Abstract

The authors have requested that this preprint be removed from Research Square.
Breaking the hard-sphere model with fluorite and antifluorite solid solutions
Authors: Vauchy Romain , Hirooka Shun , Watanabe Masashi , Kato Masato 
Published: December 2022

Abstract
Using the hard-sphere model with the existing tabulated values of ionic radii to calculate the lattice parameters of minerals does not always match experimental data. An adaptation of this crystallographic model is proposed by considering the cations and anions as hard and soft spheres, respectively. We demonstrate the relevance of this “hybrid model” with fluorite and antifluorite-structured systems.
Scheme Selection based on Clusters' Quality in Multi-Clustering M-CCF Recommender System
Authors: Kużelewska Urszula 
Published: December 2022

Abstract
Identification of a neighbourhood that is based on multi-clusters was successfully applied to recommender systems, increasing recommendation accuracy and eliminating divergence related to differences in clustering schemes generated by traditional methods. Multi-Clustering Collaborative Filtering algorithm was developed for this purpose that was described in the author's previous papers. However, the solutions involving many clusters face substantial challenges around memory consumption and scalability. Differently, they do not equally take advantage of all the partitionings. Selection of the clusters to forward to the recommender system's input, without deterioration in recommendation accuracy, can be used as a precaution to address these problems. The article describes a solution of a clustering schemes' selection based on internal indices evaluation, that can be applied for input data preparation in collaborative filtering recommender systems. The results reported in this paper confirmed its positive impact on the system's overall recommendation performance, which usually increases after the selection of schemes. Additionally, a smaller number of clustering schemes on an input of a recommender system improves its scalability including memory consumption. The obtained values were compared with baseline recommenders' outcomes.
A blood-based panel of DNA methylation markers improves diagnosis accuracy of Alzheimer’s disease
Authors: Acha Blanca , Corroza Jon , de Gordoa Javier Sánchez-Ruiz , Zueco Sara , Robles Maitane , Méndez-López Iván , Conde Mónica Macías , Cabello Carolina , Roldan Miren , Urdánoz-Casado Amaya , Jericó Ivonne , Erro María Elena , Alcolea Daniel , LLeó Alberto , Blanco-Luquin Idoia , Mendioroz Maite 
Published: December 2022

AbstractBackground: This work investigated the diagnostic accuracy of a blood-based DNA methylation marker panel as a non-invasive tool to identify Alzheimer's disease (AD) patients.Methods: Blood DNA methylation levels at 46 CpG sites (21 genes selected after a comprehensive literature search) were measured in 80 patients with probable AD dementia and 100 age- and sex-matched controls by bisulfite pyrosequencing. Plasma pTau181 levels were determined by Simoa technology. Multivariate logistic regression analysis was performed to explore the optimal model to discriminate AD patients from controls.Results:A panel including DNA methylation levels atNXN(>0.81),TREML2(<0.27) andHOXA3(>0.91) genes and plasma pTau181 (>1.82 pg/mL) significantly improved (AUC=0.94;Pvalue < 0.01) the diagnostic performance of a single pTau181-based model, adjusted for age, sex andAPOEɛ4 genotype. After sex-stratified analysis,HOXA3DNA methylation levels showed consistently associated to AD.Conclusions:These results highlight the potential translational value of blood-based DNA methylation biomarkers for the diagnosis of AD.
NGS-defined measurable residual disease (MRD) after initial chemotherapy as a prognostic biomarker for acute myeloid leukemia
Authors: Li Yonghong , Solis-Ruiz Jose , Yang Fei , Long Nicola , Tong Carmen , Lacbawan Felicitas , Racke Frederick , Press Richard 
Published: December 2022

Abstract
Treated AML patients often have measurable residual disease (MRD) due to persisting low-level clones. This study assessed whether residual post-treatment somatic mutations, detected by NGS, were significantly prognostic for subsequent clinical outcomes. AML patients (n = 128) underwent both pre-and post-treatment testing with the same 42-gene MRD-validated NGS assay. After induction, 59 (46%) patients were mutation-negative (0.0024 VAF detection limit) and 69 (54%) had ≥ 1 persisting NGS-detectable mutation. Compared with NGS-negative patients, NGS-positive patients had shorter overall survival (17 months versus median not reached; P = 0.004; hazard ratio = 2.2 [95% CI: 1.3–3.7]) and a shorter time to relapse (14 months versus median not reached; P = 0.014; HR = 1.9 [95% CI: 1.1–3.1]). Among 95 patients with a complete morphologic remission (CR), 43 (45%) were MRD-positive by NGS and 52 (55%) were MRD-negative. These MRD-positive CR patients had a shorter overall survival (16.8 months versus median not reached; P = 0.013; HR = 2.1 [95% CI: 1.2–3.9]) than did the MRD-negative CR patients. Post-treatment persisting MRD positivity, defined by the same NGS-based test used at diagnosis, is thus a more sensitive biomarker for low-level leukemic clones compared to traditional non-molecular methods and is prognostic of subsequent relapse and death.