bluuebunny/crossref_metadata_2025_split huggingface dataset:
The Perception of the Utility of Social Media by Caregivers of Persons with Autism Spectrum Disorders during a Period of Home Quarantine
Authors: Guidi Stefano , Palmitesta Paola , Bracci Margherita , Marchigiani Enrica , Parlangeli Oronzo 
Published: February 2023

Abstract
During the COVID-19 pandemic, the use of social media offered a possible way to address the difficulties of social relationships for people with autism spectrum disorder (ASD), as well as a way to ease the problems of their caregivers. To gather information on the feasibility of this solution, we conducted an online questionnaire about the first lockdown period in Italy (March–May 2020) with 29 caregivers of ASD individuals. The questionnaire investigated their living conditions, the way time was spent during isolation, the availability of technological equipment, the perceived level of anxiety and the perceived utility of social media. The results showed that the difficulties of using social media had not been overcome, even at this time of greatest need. However, caregivers who take care of ASD people with high levels of anxiety perceived social media as more useful. This result invites further reflection on how to implement social media effectively for people with ASD.
A multi-site randomized trial of a clinical decision support intervention to improve problem list completeness
Authors: Wright Adam , Schreiber Richard , Bates David W , Aaron Skye , Ai Angela , Cholan Raja Arul , Desai Akshay , Divo Miguel , Dorr David A , Hickman Thu-Trang , Hussain Salman , Just Shari , Koh Brian , Lipsitz Stuart , Mcevoy Dustin , Rosenbloom Trent , Russo Elise , Ting David Yut-Chee , Weitkamp Asli , Sittig Dean F 
Published: February 2023

Abstract

Objective
To improve problem list documentation and care quality.

Materials and methods
We developed algorithms to infer clinical problems a patient has that are not recorded on the coded problem list using structured data in the electronic health record (EHR) for 12 clinically significant heart, lung, and blood diseases. We also developed a clinical decision support (CDS) intervention which suggests adding missing problems to the problem list. We evaluated the intervention at 4 diverse healthcare systems using 3 different EHRs in a randomized trial using 3 predetermined outcome measures: alert acceptance, problem addition, and National Committee for Quality Assurance Healthcare Effectiveness Data and Information Set (NCQA HEDIS) clinical quality measures.

Results
There were 288 832 opportunities to add a problem in the intervention arm and the problem was added 63 777 times (acceptance rate 22.1%). The intervention arm had 4.6 times as many problems added as the control arm. There were no significant differences in any of the clinical quality measures.

Discussion
The CDS intervention was highly effective at improving problem list completeness. However, the improvement in problem list utilization was not associated with improvement in the quality measures. The lack of effect on quality measures suggests that problem list documentation is not directly associated with improvements in quality measured by National Committee for Quality Assurance Healthcare Effectiveness Data and Information Set (NCQA HEDIS) quality measures. However, improved problem list accuracy has other benefits, including clinical care, patient comprehension of health conditions, accurate CDS and population health, and for research.

Conclusion
An EHR-embedded CDS intervention was effective at improving problem list completeness but was not associated with improvement in quality measures.
Contrast-enhanced Mammography-guided Biopsy: Initial Trial and Experience
Authors: Kowalski Aneta , Arefan Dooman , Ganott Marie A , Harnist Kimberly , Kelly Amy E , Lu Amy , Nair Bronwyn E , Sumkin Jules H , Vargo Adrienne , Berg Wendie A , Zuley Margarita L 
Published: February 2023

AbstractObjectiveEvaluate lesion visibility and radiologist confidence during contrast-enhanced mammography (CEM)-guided biopsy.MethodsWomen with BI-RADS ≥4A enhancing breast lesions were prospectively recruited for 9-g vacuum-assisted CEM-guided biopsy. Breast density, background parenchymal enhancement (BPE), lesion characteristics (enhancement and conspicuity), radiologist confidence (scale 1–5), and acquisition times were collected. Signal intensities in specimens were analyzed. Patient surveys were collected.ResultsA cohort of 28 women aged 40–81 years (average 57) had 28 enhancing lesions (7/28, 25% malignant). Breast tissue was scattered (10/28, 36%) or heterogeneously dense (18/28, 64%) with minimal (12/28, 43%), mild (7/28, 25%), or moderate (9/28, 32%) BPE on CEM. Twelve non-mass enhancements, 11 masses, 3 architectural distortions, and 2 calcification groups demonstrated weak (12/28, 43%), moderate (14/28, 50%), or strong (2/28, 7%) enhancement. Specimen radiography demonstrated lesion enhancement in 27/28 (96%). Radiologists reported complete lesion removal on specimen radiography in 8/28 (29%). Average time from contrast injection to specimen radiography was 18 minutes (SD = 5) and, to post-procedure mammogram (PPM), 34 minutes (SD = 10). Contrast-enhanced mammography PPM was performed in 27/28 cases; 13/19 (68%) of incompletely removed lesions on specimen radiography showed residual enhancement; 6/19 (32%) did not. Across all time points, average confidence was 2.2 (SD = 1.2). Signal intensities of enhancing lesions were similar to iodine. Patients had an overall positive assessment.ConclusionLesion enhancement persisted through PPM and was visible on low energy specimen radiography, with an average “confident” score. Contrast-enhanced mammography-guided breast biopsy is easily implemented clinically. Its availability will encourage adoption of CEM.
Not welcome anymore: the effect of electoral incentives on the reception of refugees
Authors: Gamalerio Matteo , Negri Margherita 
Published: February 2023

Abstract
Do electoral incentives affect immigration policies? Exploiting the Italian system for refugees’ reception and data from Italian municipalities, we show that proximity to elections reduces the probability that a municipality applies to host a refugee center by 26%, despite the economic benefits arising from these centers. Low electoral competition and high shares of extreme-right voters drive the effect. Our results are rationalized by a theoretical model and can explain the unequal distribution of refugees across and within countries.
SOCS3 deficiency-dependent autophagy repression promotes the survival of early-stage myeloid-derived suppressor cells in breast cancer by activating the Wnt/mTOR pathway
Authors: Zhang Wenwen , Li Xingchen , Jiang Mengmeng , Ji Chenyan , Chen Guidong , Zhang Qiaoling , Liu Pengpeng , Zhang Rui , Ren Xiubao , Yu Wenwen , Yu Jinpu 
Published: February 2023

Abstract
Early-stage myeloid-derived suppressor cells are a newly defined subset of myeloid-derived suppressor cells in breast cancer tissues and related to poor prognosis in patients with breast cancer. Compared with classical myeloid-derived suppressor cells, early-stage myeloid-derived suppressor cells display exceptional immunosuppressive ability and accumulate in the tumor microenvironment to suppress innate and adaptive immunity. Previously, we demonstrated that early-stage myeloid-derived suppressor cells were SOCS3 deficiency dependent and correlated with differentiation arrest in the myeloid lineage. Autophagy is a major regulator of myeloid differentiation, but the mechanism by which autophagy regulates the development of early-stage myeloid-derived suppressor cells has not been elucidated. Here, we constructed EO771 mammary tumor-bearing conditional myeloid SOCS3 knockout mice (SOCS3MyeKO) characterized by abundant tumor-infiltrating early-stage myeloid-derived suppressor cells and exacerbated immunosuppression in vitro and in vivo. We found that early-stage myeloid-derived suppressor cells isolated from SOCS3MyeKO mice showed differentiation arrest in the myeloid lineage, which was caused by limited autophagy activation in an Wnt/mTOR-dependent manner. RNA sequencing and microRNA microarray assays revealed that miR-155–induced C/EBPβ downregulation activated the Wnt/mTOR pathway and promoted autophagy repression and differentiation arrest in early-stage myeloid-derived suppressor cells. Furthermore, inhibition of Wnt/mTOR signaling suppressed both tumor growth and the immunosuppressive functions of early-stage myeloid-derived suppressor cells. Thus, SOCS3 deficiency-dependent autophagy repression and their regulatory mechanisms could contribute to the immunosuppressive tumor microenvironment. Our study proposes a novel mechanism for promoting early-stage myeloid-derived suppressor cell survival, which might shed new light on a potential target of oncologic therapy.
The genetic architecture of soybean photothermal adaptation to high latitudes
Authors: Zhu Xintian , Leiser Willmar L , Hahn Volker , Würschum Tobias 
Published: February 2023

Abstract
Soybean is a major plant protein source for both human food and animal feed, but to meet global demands as well as a trend towards regional production, soybean cultivation needs to be expanded to higher latitudes. In this study, we developed a large diversity panel consisting of 1503 early-maturing soybean lines and used genome-wide association mapping to dissect the genetic architecture underlying two crucial adaptation traits, flowering time and maturity. This revealed several known maturity loci, E1, E2, E3, and E4, and the growth habit locus Dt2 as causal candidate loci, and also a novel putative causal locus, GmFRL1, encoding a homolog of the vernalization pathway gene FRIGIDA-like 1. In addition, the scan for quantitative trait locus (QTL)-by-environment interactions identified GmAPETALA1d as a candidate gene for a QTL with environment-dependent reversed allelic effects. The polymorphisms of these candidate genes were identified using whole-genome resequencing data of 338 soybeans, which also revealed a novel E4 variant, e4-par, carried by 11 lines, with nine of them originating from Central Europe. Collectively, our results illustrate how combinations of QTL and their interactions with the environment facilitate the photothermal adaptation of soybean to regions far beyond its center of origin.
Hemispheric distribution of coronal mass ejections from 1996 to 2020
Authors: Zhang X J , Deng L H , Qiang Z P , Fei Y , Tian X A , Li C 
Published: February 2023

ABSTRACT
Solar magnetic structures are known to be asymmetrically distributed between the two hemispheres. To date, the hemispheric variations of the coronal mass ejections (CMEs) at different latitudes, in different cycles, and for different types (regular and specific events) are still unclear. From the list of white-light CMEs in the Coordinated Data Analysis Web catalogue, we investigate the hemispheric asymmetry of high-latitude and low-latitude CMEs in the time interval from 1996 January to 2020 December. The main results are shown for the following: (1) in each hemisphere, regular CMEs are significantly correlated with solar activity, particularly for low latitudes. However, specific CMEs are not correlated with solar activity; (2) the main reason for the hemispheric asymmetry of the CMEs is attributable to specific CMEs, not regular CMEs. The hemispheric asymmetry of high-latitude CMEs appears to have little connection to that of low-latitude CMEs; (3) for the total and specific CMEs, the relationship between the absolute asymmetry index at high and low latitudes has a positive correlation prior to the cycle maximum, but a negative correlation after the cycle maximum; and (4) the dominant hemisphere, the cumulative trend, and the amplitude of the total, specific, and regular CMEs in cycle 23 differ from those in cycle 24. Our analysis results could be useful for understanding the cyclical variation of the magnetic free energy during different solar cycles, and could also provide insight into more physical processes responsible for the solar–terrestrial relationship.
INSPIRE: INvestigating Stellar Population In RElics – IV. The initial mass function slope in relics
Authors: Martín-Navarro Ignacio , Spiniello C , Tortora C , Coccato L , D’Ago G , Ferré-Mateu A , Pulsoni C , Hartke J , Arnaboldi M , Hunt L , Napolitano N R , Scognamiglio D , Spavone M 
Published: February 2023

ABSTRACTIn the last decade, growing evidence has emerged supporting a non-universal stellar initial mass function (IMF) in massive galaxies, with a larger number of dwarf stars with respect to the Milky Way (bottom-heavy IMF). However, a consensus about the mechanisms that cause IMF variations is yet to be reached. Recently, it has been suggested that stars formed early-on in cosmic time, via a star formation burst, could be characterized by a bottom-heavy IMF. A promising way to confirm this is to use relics, ultra-compact massive galaxies, almost entirely composed by these ‘pristine’ stars. The INvestigating Stellar Population In RElics (INSPIRE) Project aims at assembling a large sample of confirmed relics, that can serve as laboratory to investigate on the conditions of star formation in the first 1–3 Gyr of the Universe. In this third INSPIRE paper, we build a high signal-to-noise spectrum from five relics, and one from five galaxies with similar sizes, masses, and kinematical properties, but characterized by a more extended star formation history (non-relics). Our detailed stellar population analysis suggests a systematically bottom-heavier IMF slope for relics than for non-relics, adding new observational evidence for the non-universality of the IMF at various redshifts and further supporting the above proposed physical scenario.
Spectro-temporal and type I X-ray burst analysis of GX 3+1 using AstroSat observations
Authors: Thomas Neal Titus , Gudennavar S B , Bubbly S G 
Published: February 2023

ABSTRACT
GX 3+1, an atoll type neutron star low-mass X-ray binary, was observed four times by Soft X-ray Telescope and The Large Area X-ray Proportional Counter on-board AstroSat between 2017 October 5 and 2018 August 9. The hardness-intensity-diagram of the source showed it to be in the soft spectral state during all the four observations. The spectra of the source could be adequately fit with a model consisting of blackbody ($\tt {bbody}$) and power--law ($\tt {powerlaw}$) components. This yielded the blackbody radius and mass accretion rate to be ∼8 km and ∼2 × 10−9 M⊙ y−1, respectively. In one of the observations, a type I X-ray burst having a rise and e-folding time of 0.6 and 5.6 s, respectively, was detected. Time-resolved spectral analysis of the burst showed that the source underwent a photospheric radius expansion. The radius of the emitting blackbody in GX 3+1 and its distance were estimated to be 9.19 $\substack{+0.97\\ -0.82}$ km and 10.17 $\substack{+0.07\\ -0.18}$ kpc, respectively. Temporal analysis of the burst yielded upper limits of the fractional root mean square amplitude of 7${{\ \rm per\ cent}}$, 5${{\ \rm per\ cent}}$, and 6${{\ \rm per\ cent}}$ during burst start, burst maximum, and right after the radius expansion phase, respectively.
Genome Size Changes by Duplication, Divergence, and Insertion inCaenorhabditisWorms
Authors: Adams Paula E , Eggers Victoria K , Millwood Joshua D , Sutton John M , Pienaar Jason , Fierst Janna L 
Published: February 2023

AbstractGenome size has been measurable since the 1940s but we still do not understand genome size variation. Caenorhabditis nematodes show strong conservation of chromosome number but vary in genome size between closely related species. Androdioecy, where populations are composed of males and self-fertile hermaphrodites, evolved from outcrossing, female-male dioecy, three times in this group. In Caenorhabditis, androdioecious genomes are 10–30% smaller than dioecious species, but in the nematode Pristionchus, androdioecy evolved six times and does not correlate with genome size. Previous hypotheses include genome size evolution through: 1) Deletions and “genome shrinkage” in androdioecious species; 2) Transposable element (TE) expansion and DNA loss through large deletions (the “accordion model”); and 3) Differing TE dynamics in androdioecious and dioecious species. We analyzed nematode genomes and found no evidence for these hypotheses. Instead, nematode genome sizes had strong phylogenetic inertia with increases in a few dioecious species, contradicting the “genome shrinkage” hypothesis. TEs did not explain genome size variation with the exception of the DNA transposon Mutator which was twice as abundant in dioecious genomes. Across short and long evolutionary distances Caenorhabditis genomes evolved through small structural mutations including gene-associated duplications and insertions. Seventy-one protein families had significant, parallel decreases across androdioecious Caenorhabditis including genes involved in the sensory system, regulatory proteins and membrane-associated immune responses. Our results suggest that within a dynamic landscape of frequent small rearrangements in Caenorhabditis, reproductive mode mediates genome evolution by altering the precise fates of individual genes, proteins, and the phenotypes they underlie.
PBRM1 bromodomains associate with RNA to facilitate chromatin association
Authors: De Silva Saumya M , Dhiman Alisha , Sood Surbhi , Mercedes Kilsia F , Simmons William J , Henen Morkos A , Vögeli Beat , Dykhuizen Emily C , Musselman Catherine A 
Published: February 2023

Abstract
PBRM1 is a subunit of the PBAF chromatin remodeling complex, which is mutated in 40–50% of clear cell renal cell carcinoma patients. It is thought to largely function as a chromatin binding subunit of the PBAF complex, but the molecular mechanism underlying this activity is not fully known. PBRM1 contains six tandem bromodomains which are known to cooperate in binding of nucleosomes acetylated at histone H3 lysine 14 (H3K14ac). Here, we demonstrate that the second and fourth bromodomains from PBRM1 also bind nucleic acids, selectively associating with double stranded RNA elements. Disruption of the RNA binding pocket is found to compromise PBRM1 chromatin binding and inhibit PBRM1-mediated cellular growth effects.
DNA polymerase θ-mediated repair of high LET radiation-induced complex DNA double-strand breaks
Authors: Yi Geunil , Sung Yubin , Kim Chanwoo , Ra Jae Sun , Hirakawa Hirokazu , Kato Takamitsu A , Fujimori Akira , Kim Hajin , Takata Kei-ichi 
Published: February 2023

AbstractDNA polymerase θ (POLQ) is a unique DNA polymerase that is able to perform microhomology-mediated end-joining as well as translesion synthesis (TLS) across an abasic (AP) site and thymine glycol (Tg). However, the biological significance of the TLS activity is currently unknown. Herein we provide evidence that the TLS activity of POLQ plays a critical role in repairing complex DNA double-strand breaks (DSBs) induced by high linear energy transfer (LET) radiation. Radiotherapy with high LET radiation such as carbon ions leads to more deleterious biological effects than corresponding doses of low LET radiation such as X-rays. High LET-induced DSBs are considered to be complex, carrying additional DNA damage such as AP site and Tg in close proximity to the DSB sites. However, it is not clearly understood how complex DSBs are processed in mammalian cells. We demonstrated that genetic disruption of POLQ results in an increase of chromatid breaks and enhanced cellular sensitivity following treatment with high LET radiation. At the biochemical level, POLQ was able to bypass an AP site and Tg during end-joining and was able to anneal two single-stranded DNA tails when DNA lesions were located outside the microhomology. This study offers evidence that POLQ is directly involved in the repair of complex DSBs.
Allosteric effects ofE. coliSSB and RecR proteins on RecO protein binding to DNA
Authors: Shinn Min Kyung , Chaturvedi Sumit K , Kozlov Alexander G , Lohman Timothy M 
Published: February 2023

AbstractEscherichia coli single stranded (ss) DNA binding protein (SSB) plays essential roles in DNA maintenance. It binds ssDNA with high affinity through its N-terminal DNA binding core and recruits at least 17 different SSB interacting proteins (SIPs) that are involved in DNA replication, recombination, and repair via its nine amino acid acidic tip (SSB-Ct). E. coli RecO, a SIP, is an essential recombination mediator protein in the RecF pathway of DNA repair that binds ssDNA and forms a complex with E. coli RecR protein. Here, we report ssDNA binding studies of RecO and the effects of a 15 amino acid peptide containing the SSB-Ct monitored by light scattering, confocal microscope imaging, and analytical ultracentrifugation (AUC). We find that one RecO monomer can bind the oligodeoxythymidylate, (dT)15, while two RecO monomers can bind (dT)35 in the presence of the SSB-Ct peptide. When RecO is in molar excess over ssDNA, large RecO–ssDNA aggregates occur that form with higher propensity on ssDNA of increasing length. Binding of RecO to the SSB-Ct peptide inhibits RecO–ssDNA aggregation. RecOR complexes can bind ssDNA via RecO, but aggregation is suppressed even in the absence of the SSB-Ct peptide, demonstrating an allosteric effect of RecR on RecO binding to ssDNA. Under conditions where RecO binds ssDNA but does not form aggregates, SSB-Ct binding enhances the affinity of RecO for ssDNA. For RecOR complexes bound to ssDNA, we also observe a shift in RecOR complex equilibrium towards a RecR4O complex upon binding SSB-Ct. These results suggest a mechanism by which SSB recruits RecOR to facilitate loading of RecA onto ssDNA gaps.
A genome-wide map of DNA replication at single-molecule resolution in the malaria parasitePlasmodium falciparum
Authors: Totañes Francis Isidore Garcia , Gockel Jonas , Chapman Sarah E , Bártfai Richárd , Boemo Michael A , Merrick Catherine J 
Published: February 2023

AbstractThe malaria parasite Plasmodium falciparum replicates via schizogony: an unusual type of cell cycle involving asynchronous replication of multiple nuclei within the same cytoplasm. Here, we present the first comprehensive study of DNA replication origin specification and activation during Plasmodium schizogony. Potential replication origins were abundant, with ORC1-binding sites detected every ∼800 bp. In this extremely A/T-biased genome, the sites were biased towards areas of higher G/C content, and contained no specific sequence motif. Origin activation was then measured at single-molecule resolution using newly developed DNAscent technology: a powerful method of detecting replication fork movement via base analogues in DNA sequenced on the Oxford Nanopore platform. Unusually, origins were preferentially activated in areas of low transcriptional activity, and replication forks also moved fastest through lowly transcribed genes. This contrasts with the way that origin activation is organised in other systems, such as human cells, and suggests that P. falciparum has evolved its S-phase specifically to minimise conflicts between transcription and origin firing. This may be particularly important to maximise the efficiency and accuracy of schizogony, with its multiple rounds of DNA replication and its absence of canonical cell-cycle checkpoints.