bluuebunny/crossref_metadata_2025_split huggingface dataset:
The role of lipid rafts in vesicle formation
Authors: Sapoń Karolina , Mańka Rafał , Janas Teresa , Janas Tadeusz 
Published: May 2023

ABSTRACT
The formation of membrane vesicles is a common feature in all eukaryotes. Lipid rafts are the best-studied example of membrane domains for both eukaryotes and prokaryotes, and their existence also is suggested in Archaea membranes. Lipid rafts are involved in the formation of transport vesicles, endocytic vesicles, exocytic vesicles, synaptic vesicles and extracellular vesicles, as well as enveloped viruses. Two mechanisms of how rafts are involved in vesicle formation have been proposed: first, that raft proteins and/or lipids located in lipid rafts associate with coat proteins that form a budding vesicle, and second, vesicle budding is triggered by enzymatic generation of cone-shaped ceramides and inverted cone-shaped lyso-phospholipids. In both cases, induction of curvature is also facilitated by the relaxation of tension in the raft domain. In this Review, we discuss the role of raft-derived vesicles in several intracellular trafficking pathways. We also highlight their role in different pathways of endocytosis, and in the formation of intraluminal vesicles (ILVs) through budding inwards from the multivesicular body (MVB) membrane, because rafts inside MVB membranes are likely to be involved in loading RNA into ILVs. Finally, we discuss the association of glycoproteins with rafts via the glycocalyx.
The histone methyltransferase NSD3 contributes to sister chromatid cohesion and to cohesin loading at mitotic exit
Authors: Eot-Houllier Grégory , Magnaghi-Jaulin Laura , Bourgine Gaëlle , Smagulova Fatima , Giet Régis , Watrin Erwan , Jaulin Christian 
Published: May 2023

ABSTRACT
Sister chromatid cohesion is a multi-step process implemented throughout the cell cycle to ensure the correct transmission of chromosomes to daughter cells. Although cohesion establishment and mitotic cohesion dissolution have been extensively explored, the regulation of cohesin loading is still poorly understood. Here, we report that the methyltransferase NSD3 is essential for mitotic sister chromatid cohesion before mitosis entry. NSD3 interacts with the cohesin loader complex kollerin (composed of NIPBL and MAU2) and promotes the chromatin recruitment of MAU2 and cohesin at mitotic exit. We also show that NSD3 associates with chromatin in early anaphase, prior to the recruitment of MAU2 and RAD21, and dissociates from chromatin when prophase begins. Among the two NSD3 isoforms present in somatic cells, the long isoform is responsible for regulating kollerin and cohesin chromatin-loading, and its methyltransferase activity is required for efficient sister chromatid cohesion. Based on these observations, we propose that NSD3-dependent methylation contributes to sister chromatid cohesion by ensuring proper kollerin recruitment and thus cohesin loading.
Warmer and more acidic conditions enhance performance of an endemic low-shore gastropod
Authors: Martin Nicole , Robinson Tamara B. , Clusella-Trullas Susana 
Published: May 2023

ABSTRACT
Changing ocean temperatures are predicted to challenge marine organisms, especially when combined with other factors, such as ocean acidification. Acclimation, as a form of phenotypic plasticity, can moderate the consequences of changing environments for biota. Our understanding of how altered temperature and acidification together influence species’ acclimation responses is, however, limited compared with that of responses to single stressors. This study investigated how temperature and acidification affect the thermal tolerance and righting speed of the girdled dogwhelk, Trochia cingulata. Whelks were acclimated for 2 weeks to combinations of three temperatures (11°C: cold, 13°C: moderate and 15°C: warm) and two pH regimes (8.0: moderate and 7.5: acidic). We measured the temperature sensitivity of the righting response by generating thermal performance curves from individual data collected at seven test temperatures and determined critical thermal minima (CTmin) and maxima (CTmax). We found that T. cingulata has a broad basal thermal tolerance range (∼38°C) and after acclimation to the warm temperature regime, both the optimal temperature for maximum righting speed and CTmax increased. Contrary to predictions, acidification did not narrow this population's thermal tolerance but increased CTmax. These plastic responses are likely driven by the predictable exposure to temperature extremes measured in the field which originate from the local tidal cycle and the periodic acidification associated with ocean upwelling in the region. This acclimation ability suggests that T. cingulata has at least some capacity to buffer the thermal changes and increased acidification predicted to occur with climate change.
A transit map for micro-scale urban development in Alexandria, Egypt
Authors: H. Seoudy Mohamed , El Menshawy Adel , El Adawy Amr 
Published: May 2023

Background: Due to Egypt's strategic location among countries, transportation is one of the most significant development sectors because it plays a major part in today's economy and society and has a large influence on growth and employment. Over the years, the Egyptian General Organisation of Physical Planning (GOPP) has prepared strategic general urban plans in collaboration with local and foreign organisations, including transportation plans. The constant focus of authorities on strategic plans and their inability to implement them on schedule are a major issue. In other words, they always take development from a distant perspective and do not deal with the main problem that exists within cities, as the existing micro-scale transit built environments (MSTBEs) of cities are not ready due to a lack of transit-oriented communities (TOCs), sustainably developed transit supply systems, and mobility hubs. Methods: The "Enhanced MSTBE Phases" methodology is used for the key elements of the study design used in this research, depending on data collection, approvals, techniques, and analysis methods. As a case study, these key elements are in the documentation, analysis, and development of the Muharram Bek El Mowkaf El Gedid Mobility Hub (MBMH) and the 800 m radius around it. Results: The results indicate that Enhanced MSTBE Phases led to the establishment of the MBMH and the 800 m radius surrounding it as a sustainable MSTBE in Alexandria, Egypt, which is chosen as the case study. Conclusions: The development of this MSTBE is a catalyst for future effects that will have a long-term impact on meso-scale and, ultimately, macro-scale transit built environments.
Benefits, anxieties, acceptance, and barriers to the new injectable contraceptive DMPA-SC (Sayana Press): Clients’ perceptions in Sindh, Pakistan
Authors: Veesar Ghulam Yaseen , Lashari Talib , Fida Roshan , Veesar Mansoor Ahmed 
Published: May 2023

Background: Injections are Pakistan's third most preferred contraceptive method because of their convenience. They represent a 2.5% share of the contraceptive prevalence rate (CPR) and contribute 10% to the current CPR. However, injections require a clinical setting or a healthcare provider for administration. A new method of subcutaneous presentation of depot medroxyprogesterone acetate (DMPA-SC), namely Sayana Press in Uniject™, has been introduced, which can be administered in nonclinical settings or self-administered by trained women. This study examined clients’ perceptions of the current depot medroxyprogesterone acetate-intramuscular injection (DMPA-IM) and its accessibility, availability, affordability, advantages, and disadvantages. In addition, it explored the benefits, barriers, and challenges regarding the new method of DMPA-SC (Sayana Press), especially considering self-injection. Methods: The study was conducted in Sindh, Pakistan. Three focus group discussions were conducted with 9–13 female participants with different demographic characteristics. A semi-structured questionnaire was used. The discussion was recorded, transcribed, and translated from Urdu or Sindhi to English. Transcripts were coded precisely, and data analysis was performed using NVivo software. Results: Participants expressed moderate fear of self-injection and risk of an inaccurate prick, suggesting that DMPA-SC acceptance may not be challenging at a community level. They appreciated free services at public health facilities, as the affordability of private facilities may be challenging for those with low income. Most participants agreed to pay PKR 50–300 (approximately 1 USD or less) as service charges for a private facility, while some agreed to pay for transportation costs when lacking alternative methods. Conclusions: DMPA-SC is a valuable alternative, provided its challenges are adequately addressed. Information about self-injection contraceptives is currently limited, and shared self-administration may be difficult without adequate training and counseling. Nevertheless, clients prefer the self-injection method for family planning to avoid transportation and private service charges.
Optimal Pricing with a Single Point
Authors: Allouah Amine , Bahamou Achraf , Besbes Omar 
Published: May 2023

Historical data are typically limited. We study the following fundamental data-driven pricing problem. How can/should a decision maker price its product based on data at a single historical price? How valuable is such data? We consider a decision maker who optimizes over (potentially randomized) pricing policies to maximize the worst-case ratio of the garnered revenue compared to an oracle with full knowledge of the distribution of values, when the latter is only assumed to belong to a broad nonparametric set. In particular, our framework applies to the widely used regular and monotone nondecreasing hazard rate (mhr) classes of distributions. For settings where the seller knows the exact probability of sale associated with one historical price or only a confidence interval for it, we fully characterize optimal performance and near-optimal pricing algorithms that adjust to the information at hand. The framework we develop is general and allows to characterize optimal performance for deterministic or more general randomized mechanisms and leads to fundamental novel insights on the value of data for pricing. As examples, against mhr distributions, we show that it is possible to guarantee 85% of oracle performance if one knows that half of the customers have bought at the historical price, and if only 1% of the customers bought, it still possible to guarantee 51% of oracle performance. This paper was accepted by David Simchi-Levi, revenue management and market analytics. Supplemental Material: The data files and online appendices are available at https://doi.org/10.1287/mnsc.2023.4683 .
The Information in Hedge Fund Option Holdings
Authors: Anand Amber , Hua Jian , Puckett Andy 
Published: May 2023

We provide new insights on how hedge funds use options and short-selling channels to trade on their negative information. Bearish information in hedge fund option positions is economically large, distinct from information in short interest, and it is the combination of option positions and short interest that provides the strongest information signal. A portfolio of stocks with high short interest and bearish hedge fund option positions predicts negative abnormal returns that are more than four times as large as the portfolio with high short interest and bullish options positions. The information in hedge fund option positions increases during periods of market stress, whereas that in short interest does not. This increase is concentrated in capital-constrained hedge funds, suggesting that options provide a channel for capital-constrained hedge funds to exploit their information advantage. This paper was accepted by Lukas Schmid, finance. Funding: J. Hua received support for this project from a PSC-CUNY Award, jointly funded by The Professional Staff Congress and The City University of New York. Supplemental Material: The online appendix and data are available at https://doi.org/10.1287/mnsc.2023.4785 .
The Challenge of Maintaining Passion for Work over Time: A Daily Perspective on Passion and Emotional Exhaustion
Authors: Bredehorst Joy , Krautter Kai , Meuris Jirs , Jachimowicz Jon M. 
Published: May 2023

Passion for work is highly coveted, but many employees report struggling to maintain their passion over time. In the current research, we explain the challenge of pursuing passion by conceptualizing passion as an attribute with temporal variation. Viewed through a daily lens, we argue that self-regulation plays a critical role in understanding the challenges underlying the daily maintenance of passion. More specifically, we hypothesize that—unless employees adequately regulate their passion on any given day—higher levels of passion will lead them to invest more time and energy into their work, decreasing their psychological detachment from work after the workday, and consequently resulting in higher levels of emotional exhaustion the next day. Higher levels of emotional exhaustion on a given day subsequently prompt a greater need for recovery, shifting employees’ focus away from devoting time and energy into work, thereby eroding their passion on the following day. Two daily-diary studies covering 30 and 10 consecutive working days provided support for our predictions (Ntotal= 798; ktotal= 15,702). Employees who felt more in charge of their passion on any given day engaged in self-regulation during their workday, increasing their psychological detachment from work and subsequently being less likely to suffer the detrimental consequences of higher daily passion. Our theory and findings demonstrate the daily interplay between passion and emotional exhaustion and specify why passion may be self-limiting unless employees adequately manage it, reflecting a challenge they need to navigate each day in pursuing their passion.Supplemental Material: The online appendix is available at https://doi.org/10.1287/orsc.2023.1673 .
Cardiac arrest due to out-of-hospital pulmonary embolism during pregnancy: successful thrombolysis
Authors: Söderberg Mårten , Smedberg Erica , Lindqvist Pelle G. 
Published: May 2023

Introduction: Pulmonary embolism (PE) is a rare, severe complication in pregnancy, in which case thrombolysis can be lifesaving but has risks. We aim to highlight actions specific to pregnant women. Case Description: A 24-week pregnant woman developed shortness of breath and experienced sudden cardiac arrest. Cardiopulmonary resuscitation (CPR) was begun immediately in the ambulance and a perimortem caesarean section was performed upon arrival at hospital, but the new-born died. After 55 minutes of CPR, bedside echocardiography revealed right ventricular strain and thrombolysis was given. The uterus was bandaged to minimize blood loss. After massive transfusions and correction of haemostasis, a hysterectomy was performed due to inability of the uterus to contract. After 3 weeks, the patient was discharged in good health and placed on continuous anticoagulant treatment with warfarin. Discussion: Approximately 3% of all out-of-hospital cardiac arrest cases are due to PE. Among the few patients who survive at the scene, thrombolysis can be lifesaving and should be considered in pregnant women with unstable PE. Prompt collaborative diagnostic work-up in the emergency room is necessary. In a pregnant woman with cardiac arrest, a perimortem caesarean section improves the chances of both maternal and fetal survival. Conclusion: Thrombolysis should be considered for patients with PE in pregnancy with the same indications as in a non-pregnant woman. In case of survival, there is profuse bleeding with need for massive transfusions and haemostasis correction. Despite being in very poor condition, the above patient survived and was fully restored to health.
Percepções e Vivências de Psicólogos/as sobre a Punição Física em Crianças
Authors: França Tatiane , Hohendorff Jean Von , Silva Ana Claudia Pinto da , Costa Carlos 
Published: May 2023

Este estudo qualitativo e transversal investigou as percepções e vivências dos/as psicólogos/as sobre o uso da punição física em crianças. Participaram do estudo 13 psicólogos/as brasileiros/as, que responderam a um questionário sociodemográfico on-line, um roteiro de entrevista e vinhetas de situação-problema. A análise de conteúdo revelou duas categorias: Ambivalência nas percepções sobre o uso da punição física: A palmada não é uma forma de agressão?; e Reprodução da punição física geração após geração: a forma de educar mudou?. Constataram-se percepções endossando o uso da punição física e experiências pessoais de punição física na infância, mas vários participantes buscam não repetir com os filhos e são desfavoráveis a todos os níveis dessa prática. Participantes favoráveis ao uso, principalmente da palmada, demonstraram desconhecimento da sua ineficácia enquanto método educativo e de seus efeitos prejudiciais. A transgeracionalidade e a naturalização da palmada foram motivos salientados para a persistência do uso da punição física. São necessárias capacitações a psicólogos/as e estudantes sobre os prejuízos e alternativas à punição física em crianças.
Entre Silêncios e Formas: Censura e Pós-Censura na Arte Brasileira Contemporânea
Authors: Rech Morgana , Birman Joel 
Published: May 2023

O artigo trata da censura na arte brasileira contemporânea que, desde 2016, com o encerramento da exposição Queermuseu, marcou o retorno de um tipo de ato censório (pós-censura) que difere da censura praticada desde a redemocratização. Para tal, apresentamos um retorno até as formas da censura institucionalizada na Ditadura Militar (censura clássica) e sua dissolução na cultura. Com apoio em trechos de algumas entrevistas que realizamos em nosso campo empírico, a articulação teórica é feita entre os campos da estética, da política e da psicanálise, com apoio principal nas teorias de Michel Foucault, Jacques Rancière e Sigmund Freud. Argumentamos que a censura expõe de forma inequívoca o fato de que a criação responde às transformações no campo político que, por sua vez, geram novas formas artísticas. Conclui-se que a prinicipal marca da pós-censura é a imprecisão da figura do censor e que, tanto num como noutro contexto, a censura impede o exercício da função social da arte, questão que deve ser observada na psicanálise para além da subjetividade individual do artista.
Replica symmetry breaking in a Rayleigh backscattering-based random fiber laser
Authors: Lima Bismarck Costa , Tovar Pedro , Moura André L. , von der Weid Jean Pierre 
Published: May 2023

Replica symmetry breaking (RSB) phase transition is observed in disordered photonics systems. The key requirements to observe RSB are provided by random lasers, i.e., gain and disorder. However, in a random fiber laser (RFL) with scattering provided by Rayleigh scattering of light, the condition of quenched disorder is not established due to environment perturbations. Here, RSB is observed in a Rayleigh backscattering-based RFL, where light scattering is due to the inhomogeneity of the optical fiber refractive index. This is done by using short-cavity erbium-RFL with reduced interaction with the environment. Spectral characterization is made, and narrow mode linewidths are demonstrated. The intensity dynamics and laser mode behavior are analyzed showing the presence of gain competition. The results indicate a connection between the replica symmetry or RSB, Lévy-like behavior of intensities, and the presence or not of correlated laser intensity fluctuations.
Aluminum nitride waveguide beam splitters for integrated quantum photonic circuits
Authors: Jang Hyeong-Soon , Lee Donghwa , Heo Hyungjun , Kim Yong-Su , Lim Hyang-Tag , Jeon Seung-Woo , Moon Sung , Kim Sangin , Han Sang-Wook , Jung Hojoong 
Published: May 2023

We demonstrate integrated photonic circuits for quantum devices using sputtered polycrystalline aluminum nitride (AlN) on insulator. On-chip AlN waveguide directional couplers, which are one of the most important components in quantum photonics, are fabricated and show the output power splitting ratios from 50:50 to 99:1. Polarization beam splitters with an extinction ratio of more than 10 dB are also realized from the AlN directional couplers. Using the fabricated AlN waveguide beam splitters, we observe Hong–Ou–Mandel interference with a visibility of 91.7%±5.66%.
Broadband omnidirectional visible spectral metamaterials
Authors: Zhao Jing , Wu Xianfeng , Cao Di , Zhou Mingchao , Shen Zhijie , Zhao Xiaopeng 
Published: May 2023

Optical metamaterials offer the possibility of controlling the behavior of photons similarly to what has been done about electrons in semiconductors. However, most optical metamaterials are narrowband, and they achieve negative refraction within a small window of incident angles, making them impractical for common visible light systems that operate effectively over a wide range of frequencies and directions. Considerable resistive loss at the resonant frequency of these metamaterials further prevents them from being deployed in the real world. Here, we develop a novel metamaterial randomly assembled by a list of narrowband, omnidirectional, and ultralow-loss meta-cluster systems using a bottom-up approach. Weak interactions among numerous meta-cluster sets greatly broaden the effective bandwidth of the overall structure, exhibiting frequency selectivity and spatial modulation when responding to white-light illumination. We observe negative refraction in the 490–730 nm band, and observe an inverse Doppler effect at green, yellow, and red frequencies, across most of the visible spectrum. Our method allows for low-cost fabrication of sizable broadband omnidirectional three-dimensional metamaterial samples, which opens the door to the rapid development of optical metamaterials, micro–nano assembly and preparation, tunable optical device engineering, etc.
High-efficiency reflector-less dual-level silicon photonic grating coupler
Authors: Vitali Valerio , Domínguez Bucio Thalía , Lacava Cosimo , Marchetti Riccardo , Mastronardi Lorenzo , Rutirawut Teerapat , Churchill Glenn , Faneca Joaquín , Gates James C. , Gardes Frederic , Petropoulos Periklis 
Published: May 2023

We present the design and experimentally demonstrate a dual-level grating coupler with subdecibel efficiency for a 220 nm thick silicon photonics waveguide which was fabricated starting from a 340 nm silicon-on-insulator wafer. The proposed device consists of two grating levels designed with two different linear apodizations, with opposite chirping signs, and whose period is varied for each scattering unit. A coupling efficiency of −0.8  dB at 1550 nm is experimentally demonstrated, which represents the highest efficiency ever reported in the telecommunications C-band in a single-layer silicon grating structure without the use of any backreflector or index-matching material between the fiber and the grating.