bluuebunny/crossref_metadata_2025_split huggingface dataset:
Management for Arthrofibrosis Following ACL Reconstruction
Authors: Sutanto Ronny 
Published: February 2023

Introduction: Arthrofibrosis following ACL reconstruction is a debilitating complication. The incidence range from 2% to as high 35%. This Loss of motion not only cause of anterior knee pain but also inability to return to sports, and persistent weakness. The emergence of intra-articular scar tissue adhesions, or post-traumatic arthrofibrosis, may restrict the patient’s knee motion in the early postoperative period even though many factors may contribute to the development of motion loss. It might be difficult to manage motion loss once it has been diagnosed as a condition. Better understanding of the time of surgery, surgical technique improvement, and rehabilitation protocols showed less incidence of motion loss after ACL reconstruction. Case Presentation: A 46-year-old female complains of pain, stiffness, and decreased range of motion following arthroscopy ACL reconstruction of her left knee. Local examination showed diffused firm swelling over the anteromedial aspect of the knee and limitation of motion to mobilize the patella in all directions. Her bad rehabilitation program after surgery caused the main loss of her knee motion and the development of pain. Arthroscopic lysis of adhesions with manipulation under anesthesia was conducted. Following the surgery, graft observation was normal. A cyclops located between the infrapatellar pouch and medial gutter was observed and excised. Passive range of motion was also checked intra-operatively. Immediately after surgery, intensive physiotherapy was begun. The patient was very compliant with the rehabilitation program and the clinic follow-up schedule. Gradually, the patient started to regain her active full extension and flexion. Result: The functional outcome score showed improvement at the time of final follow-up, as did the average pain scores. Conclusion: Arthrofibrosis is multifactorial etiology. Knee joint ROM is better maximized by arthroscopic debridement and lysis of adhesions following arthrofibrosis after ACLR in conjunction with an intensive post-operative physical therapy plan.
How to Deal with Cartilage Lesions Based on the Size
Authors: Warindra Taufin 
Published: February 2023

Cartilage lesions are problematic because hyaline articular cartilage has limited ability to regenerate in response to damage. The size of the lesions is a determining factor in every algorithm of decision-making in terms of treatment. The decision if a cartilage lesion demands surgical treatment is depending on a variety of parameters and should always be reached mutually between physician and patient after thorough education about the risks and benefits of operative and conservative treatment options. Lesion characterization is often accomplished by the interpretation of advanced imaging studies (e.g., radiographs and magnetic resonance imaging [MRI]) and direct arthroscopic examination, which remains the gold standard to assess chondral/osteochondral pathology. A wide range of options for cartilage lesions is practised nowadays, ranging from conservative measures to simple arthroscopic interventions, marrow tapping techniques, osteochondral auto/allografting, cell-based techniques, growth factors, and emerging gene therapy techniques. Once a cartilage lesion is identified, multiple variables must be evaluated when determining treatment options. The patient’s clinical symptoms must correlate to physical examination and imaging findings to determine if the cartilage injury is the source of the patient’s pain. In this presentation, I tried to summarize how to deal with cartilage lesions based on their size.
The Biologic Approach for Managing Cartilage Injury: Where are We Now™
Authors: Evan  
Published: February 2023

Articular cartilage or Hyaline cartilage consists of 95% type 2 collagen, water, proteoglycans and chondrocytes. Cartilage functions to decrease friction, distribute loads, resists shear/compression, and also as a shock-absorbing cushion. It has the characteristics of alymphatic, avascular, aneural, anisotropic, biphasic and viscoelastic. Cartilage can be damaged by direct trauma, loss of underlying bone structure, a genetic defect in the normal structure and chemical/enzymatic threats. Articular cartilage injuries are commonly caused by sports and recreational activities. Articular cartilage damage has inherent limited healing potential; hence it remains a challenging problem for orthopedic surgeons. There are three eras of cartilage repair technique: the pre-Autologous Chondrocyte Transplantation Era or Autologous Chondrocytes Implantation (ACI), Matrix – Assisted Chondrocytes Implantation (MACI) era and Regenerative Medicine era. In 1743 Hunter stated, “Cartilage injury is a troublesome thing and once injured is seldom repaired“. Nowadays, there are a lot of modalities for cartilage repair such as microfracture, mosaicplasty, ACI, MACI, platelet-rich plasma, bone marrow aspirate concentrate, orthokine or interleukin receptor antagonist and Matrix metalloproteinases (MMPs) inhibitor.
Arthroscopic Fixation of Posterior Cruciate Ligament Avulsion Fracture with Combined Suture Tape and Internal Bracing by Suture-bridge Technique
Authors: Chernchujit Bancha , Situmeang Adrian 
Published: February 2023

Background: Tibial-sided avulsion fracture of the posterior cruciate ligament (PCL) almost always require surgical intervention. Multiple techniques of reduction and fixation have been described in the literature from open/mini-open conventional screw fixation to the all-arthroscopic fixation techniques. Recent evidence shows that the all-arthroscopic fixation techniques had higher subjective and objective knee outcome scores with the advantage of addressing concomitant intra-articular injuries such as osteochondral injuries or meniscal problems. Objectives: We propose an arthroscopic fixation technique using suture tapes placed in a suture-bridge technique and combined it with internal bracing to fix tibial-sided avulsion fracture of the PCL. Surgical Technique: After routine diagnostic knee arthroscopy in supine position via standard anterolateral and anteromedial portal, two standard posteromedial and posterolateral portal was created. A standard PCL aiming guide was used to create the tibial tunnel. PCL was secured using two single-looped suture tapes around it with both limbs brought to the posterior compartment through either side of the PCL (medial and lateral). Internal bracing was done with suture tape secured to the femoral side using fixed suspensory device and brought to the posterior compartment through the lateral side of the PCL. All tapes are then passed through the tibial tunnel in a suture-bridge manner and fixed to a button in 30° flexion while reduction quality was constantly checked under direct visualization. Results: The suture bridge configuration provides direct bone-to-bone healing and secure enough fixation for early range of motion and power exercises. All-arthroscopic approach avoids posterior element related complications such as fragment comminution and neurovascular injuries. Conclusions: This technique can be considered as a safe and effective method for fixation of tibial-sided PCL avulsion fracture, which allows active mobilization with lowered risk of complications.
Neglected Patellar Tendon Rupture Augmentation Using Fibertape Augmentation: A Case Report
Authors: Yurianto Herbert , Utoyo Ghuna Arioharjo 
Published: February 2023

Introduction: Rupture of the patellar tendon is an uncommon problem caused by athletic activities. Delayed treatment would result in suboptimal outcomes such as quadriceps contracture, residual quadriceps weakness, and loss of knee flexion. Case presentation: 18 years old female patient with a seven-month-old injury twisting her knee comes with pain and instability and positive anterior drawer test and Lachman test. The radiology findings showed total rupture of the anterior cruciate ligament (ACL) and partial tear of the patellar tendon on the MRI right knee. We perform a direct end-to-end approximation of the patellar tendon with augmentation through the transosseous site in the patella proximally and tibial tubercle distally without using a graft with a good outcome. Discussion: This is the first reported case where a patellar tendon reconstruction is done without implants, synthetic augmentation or graft. Based on this outcome, the use of this technique could be promising for patellar tendon augmentation without graft. This might be an alternative to the less expensive method of repairing a chronic patellar tendon rupture. Conclusion: The use of fibertape augmentation provides a practical solution for treating a neglected patellar tendon rupture with concurrent ligament injury needing graft repair. We believe that this technique is easily reproducible and warrants further investigation.
The applicability research of the diagnostic criteria for 10.2 Heamodialysis-related headache in the international classification of headache disorders-3rd edition
Authors: Yang Ying , Meng Fanchao , Zhu Hanyu , Zhang Lei , Lu Guangshuang , Xiao Shaobo , He Jiaji , Yu Shengyuan , Liu Ruozhuo 
Published: February 2023

Abstract
Background
Headache during hemodialysis (HDH) is prevalent but not negligible. Despite the high prevalence of dialysis headaches, they have rarely been studied. Therefore, this study aimed to evaluate the prevalence, risk factors, and clinical characteristics of HDH and reappraise the HDH diagnostic criteria in the International Classification of Headache Disorders 3 (ICHD-3).

Methods
One hundred and fifty-four patients completed this randomized cross-sectional study. Consecutive patients who underwent haemodialysis were assessed using a semi-structured questionnaire. The patients were administered face-to-face questionnaires while undergoing dialysis.

Results
This study included 154 patients. Before commencing dialysis, 3.24% (5/154) of the patients had migraine without aura, 1.29% (2/154) had menstrual-related migraine, 0.6% (1/154) had tension-type headaches, and 0.6% (1/154) had an unclassifiable headache. One case (0.6%) of headache resolved after dialysis treatment. HDH was diagnosed in 9.09% (14/154) of the patients. Headache after haemodialysis (HAH) was reported in 6.49% (10/154) of patients. The most prevalent features of HDH were frontal or temporal location, bilateral headaches, dull and throbbing nature, and moderate severity. HDH started at a mean of 2.33 ± 0.79 h after dialysis commenced. The average headache duration was 6.56 ± 1.57 h (median = 3.0 h), with 66.67% of the patients reporting a duration of ≤4 h. HDH was more prevalent in females than males (P = 0.01, P < 0.05). Female sex was a risk factor for HDH (P = 0.01，P < 0.05).

Conclusions
The diagnostic criteria for 10.2 HDH in ICHD-3 may miss several HAH. Therefore, ICHD-3 should be revised according to the literature and further studies.
The prognostic utility of preoperative neutrophil-to-lymphocyte ratio (NLR) in patients with colorectal liver metastasis: a systematic review and meta-analysis
Authors: Li Yanqing , Xu Tianxiang , Wang Xin , Jia Xiangdong , Ren Meng , Wang Xiaoxia 
Published: February 2023

AbstractThe neutrophil-to-lymphocyte ratio is used to reflect body's inflammatory status with prognostic value in different cancers. We aimed to investigate the influence of preoperative NLR in the prognosis of CRLM patients receiving surgery using meta-analysis. Data in Cochrane Library, PubMed, Embase, and Web of Science databases created before October 2022 were recruited. Meta-analysis was carried out with RevMan 5.3 and Stata16 software, and the primary outcome indicators included overall survival (OS), and secondary outcome indicators included disease-free survival (DFS) and relapse-free survival (RFS). The pooled risk ratio (HR) and 95% confidence interval (CI) for each outcome indicator were determined using random-effects models or fixed-effects models. The pooled odds ratio (OR) and corresponding 95% confidence intervals (CI) for NLR and clinicopathological characteristics were determined with a fixed-effects model. 18 papers published between 2008 and 2022 (3184 patients in total) were included. The pooled analysis found that high preoperative NLR was correlated with poor OS (multivariate HR = 1.83, 95% CI = 1.61–2.08, p < 0.01), DFS (multivariate HR = 1.78, 95% CI = 1.16–2.71, p < 0.01) and RFS (multivariate HR = 1.46, 95% CI = 1.15–1.85, p < 0.01), but NLR was not related to clinicopathological features of CRLM patients correlation. In conclusion, NLR is an independent risk factor for poor prognosis in patients with CRLM. More large-scale clinical researches are required in the future to demonstrate the inclusion of preoperative NLR as a prognostic indicator for CRLM patients to guide postoperative adjuvant chemotherapy.
Wangbi granule as a combination therapy to achieve clinical deep remission in rheumatoid arthritis: protocol for a multicenter, triple-blind, randomised, placebo-controlled trial
Authors: Wang Jinping , Wang Zihan , Lan Tianyi , Zhang Liubo , Li Zhenbin , Wang Xinchang , Zou Qinghua , Wang Yuan , Li Yanqi , Luo Ruili , Zhang Nan , Xu Yuan , Li Mengtao , Tao Qingwen 
Published: February 2023

Abstract
Introduction
Rheumatoid arthritis (RA) is a chronic inflammatory autoimmune disease that may lead to bone erosion and disability. Although there are many biological therapies in RA treatment nowadays, such as etanercept and tofacitinib, there are still a considerable number of patients who cannot achieve clinical deep remission, which makes patients feel pain and stiffness of joints. As a traditional Chinese medicine preparation, Wangbi granule showed a synergistic role with methotrexate in the treatment of RA patients with “kidney deficiency and dampness” or “stasis blocking channels”. Therefore, it is a promising therapeutic strategy for the clinical deep remission of RA. In this study, Wangbi granule will be used as the test drug. The investigators conduct this study to evaluate the efficacy and safety of Wangbi granule in the treatment of patients who have not achieved deep remission despite the use of methotrexate and tofacitinib.

Methods and analysis
Two parallel randomized, triple-blind, placebo-controlled trials will be conducted. In six study centers, 340 eligible RA patients will be recruited and randomly allocated to either the intervention group or the control group (in a 1:1 ratio). They will receive Wangbi granule or Wangbi placebo 12.0 g each time, three times a day for 12 weeks. The primary outcome is the disease activity score derivative for 28 joints (DAS28). Secondary outcomes are patient-reported outcomes, American College of Rheumatology 50% response criteria (ACR50), fatigue scale-14 (FS-14), visual analogue scale for pain (VAS), health assessment questionnaire disability index (HAQ-DI) and biomarkers such as C-reactive protein (CRP) and erythrocyte sedimentation rate (ESR).

Expected outcomes
The success of this study will provide strong evidence to confirm the efficacy and safety of Wangbi granule in the treatment of RA.
Trial registration The trial has been registered in the ClinicalTrials Registry (NCT05540938, Date: 09/15/2022, https://clinicaltrials.gov/ct2/show/NCT05540938)
No indication for SARS-CoV-2 transmission to pet ferrets, in five cities in Poland, 2021 - antibody testing among ferrets living with owners infected with SARS-CoV-2 or free of infection
Authors: Kaczorek-Łukowska Edyta , Wernike Kerstin , Beer Martin , Blank Alicja , Małaczewska Joanna , Blank Mirosława , Jałonicka Anna , Siwicki Andrzej Krzysztof 
Published: February 2023

AbstractThe severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) was first identified in China by the end of 2019 and was responsible for a pandemic in the human population that resulted in millions of deaths worldwide. Since the beginning of the pandemic, the role of animals as spill-over or reservoir hosts was discussed. In addition to cats and dogs, ferrets are becoming increasingly popular as companion animals. Under experimental conditions, ferrets are susceptible to SARS-CoV-2 and it appears that they can also be infected through contact with a SARS-CoV-2 positive owner. However, there is still little information available regarding these natural infections. Here, we serologically tested samples collected from pet ferrets (n = 45) from Poland between June and September 2021. Of the ferrets that were included in the study, 29% (13/45) had contact with owners with confirmed SARS-CoV-2 infections. Nevertheless, SARS-CoV-2-specific antibodies could not be detected in any of the animals, independent of the infection status of the owner. The obtained results suggest that ferrets cannot be readily infected with SARS-CoV-2 under natural conditions, even after prolonged contact with infected humans. However, due to the rapid mutation rate of this virus, it is important to include ferrets in future monitoring studies.
Exosome-mediated crosstalk between epithelial cells amplifies the cell injury cascade in CaOx stone formation
Authors: Yang Yuanyuan , Hong Senyuan , Wang Qing , Wang Shaogang , Xun Yang 
Published: February 2023

Abstract
Background
Calcium oxalate (CaOx) stone disease is found worldwide. To explore the role of exosomes as a mediator of intercellular crosstalk during CaOx stone formation, we conducted this study, which may provide a new insight into the treatment and prevention of CaOx stones.

Methods
Exosomes derived from HK2 cells with (EXO(S)) or without (EXO(C))CaOx crystal stimulation were cocultured with normal tubular epithelial cells and subcapsularly injected into rat kidneys. Then, oxidative stress levels, the MAPK signalling pathway and osteogenic changes were detected via qPCR, Western blotting, immunofluorescence and immunohistochemical staining. In vivo fluorescence imaging and exosome internalization assays showed the absorption and utilization of exosomes.

Results
EXO(S) increased the reactive oxygen species (ROS) level and activated the expression of BMP2, OPN and OCN via the MAPK/P-38 pathway both in vivo and in vitro. In vivo experiments showed that preinjection of EXO(S) aggravated, while preinjection of EXO(C) ameliorated, these effects. Crystal depositions were significantly increased in SD rats injected with GAM when they were preinjected with EXO(S), and these effects could be reversed after preinjection with EXO(C).

Conclusion
Our study revealed that exosome-mediated intercellular crosstalk could accelerate the formation of CaOx stones by promoting oxidative stress and the osteogenic cascade in normal tubular epithelial cells.

Graphical Abstract
HK2 cells stimulated with CaOx crystals released more exosomal miR-223-3p and S100A8 comparing with normal HK2 cells. These exosomes derived from HK2 cells stimulated with CaOx (EXO(S)) could amplify the oxidative stress and osteogenic changes via MAPK/P-38 pathway, which finally led to the formation of Randall’s plaque.
About the dark corners in the gene function space of Escherichia coli remaining without illumination by scientific literature
Authors: Tantoso Erwin , Eisenhaber Birgit , Sinha Swati , Jensen Lars Juhl , Eisenhaber Frank 
Published: February 2023

Abstract
Background
Although Escherichia coli (E. coli) is the most studied prokaryote organism in the history of life sciences, many molecular mechanisms and gene functions encoded in its genome remain to be discovered. This work aims at quantifying the illumination of the E. coli gene function space by the scientific literature and how close we are towards the goal of a complete list of E. coli gene functions.

Results
The scientific literature about E. coli protein-coding genes has been mapped onto the genome via the mentioning of names for genomic regions in scientific articles both for the case of the strain K-12 MG1655 as well as for the 95%-threshold softcore genome of 1324 E. coli strains with known complete genome. The article match was quantified with the ratio of a given gene name’s occurrence to the mentioning of any gene names in the paper. The various genome regions have an extremely uneven literature coverage. A group of elite genes with ≥ 100 full publication equivalents (FPEs, FPE = 1 is an idealized publication devoted to just a single gene) attracts the lion share of the papers. For K-12, ~ 65% of the literature covers just 342 elite genes; for the softcore genome, ~ 68% of the FPEs is about only 342 elite gene families (GFs). We also find that most genes/GFs have at least one mentioning in a dedicated scientific article (with the exception of at least 137 protein-coding transcripts for K-12 and 26 GFs from the softcore genome). Whereas the literature growth rates were highest for uncharacterized or understudied genes until 2005–2010 compared with other groups of genes, they became negative thereafter. At the same time, literature for anyhow well-studied genes started to grow explosively with threshold T10 (≥ 10 FPEs). Typically, a body of ~ 20 actual articles generated over ~ 15 years of research effort was necessary to reach T10. Lineage-specific co-occurrence analysis of genes belonging to the accessory genome of E. coli together with genomic co-localization and sequence-analytic exploration hints previously completely uncharacterized genes yahV and yddL being associated with osmotic stress response/motility mechanisms.

Conclusion
If the numbers of scientific articles about uncharacterized and understudied genes remain at least at present levels, full gene function lists for the strain K-12 MG1655 and the E. coli softcore genome are in reach within the next 25–30 years. Once the literature body for a gene crosses 10 FPEs, most of the critical fundamental research risk appears overcome and steady incremental research becomes possible.