bluuebunny/crossref_metadata_2025_split huggingface dataset:
History of maternal migraine and its association with preeclampsia: A case–control study in a low-resource setting in Sudan, Africa
Authors: Adam Ishag , Elmugabil Abdelmageed , AlHabardi Nadiah 
Published: August 2023

Background Previous studies have reported various levels of association between migraine and preeclampsia. However, there are no published data on migraine and its association with preeclampsia in African countries, including Sudan. Methods A case–control study was conducted at Rabak Maternity Hospital in White Nile State, Central Sudan. The cases were pregnant women with preeclampsia, while the controls were healthy pregnant women. All participants were interviewed using questionnaire. The adjusted odds ratio (AOR) and a 95% confidence interval (CI) were calculated in a multivariate regression analysis. Results Of 148 pregnant women with preeclampsia, 96 (64.9%) women had mild preeclampsia and 52 (35.1%) women had severe preeclampsia. Of the 148 study participants with preeclampsia, 57 (38.5%) had a history of migraine and 19/296 (6.4%) women in control group had a history of migraine ( p < 0.001). Pregnant women with a history of migraine have higher odds of preeclampsia than pregnant women without a history of migraine (AOR = 9.01, 95% CI = 4.81–16.86). A history of preeclampsia, being overweight and obesity were associated with preeclampsia. Conclusion Our findings are consistent with the findings of previous studies on the association between migraine and preeclampsia. More studies are needed on this topic.
Parathyroid Hormone Inhibits Fatty Infiltration and Muscle Atrophy After Rotator Cuff Tear by Browning of Fibroadipogenic Progenitors in a Rodent Model
Authors: Iio Ryosuke , Manaka Tomoya , Takada Naoki , Orita Kumi , Nakazawa Katsumasa , Hirakawa Yoshihiro , Ito Yoichi , Nakamura Hiroaki 
Published: August 2023

Background: Progressive fatty infiltration and muscle atrophy after rotator cuff tears lead to tendon repair failure and poor outcomes. Fibro-adipogenic progenitors (FAPs) are involved in fatty infiltration and muscle homeostasis of skeletal muscle. Inducing FAP differentiation into brown adipocyte–like “beige adipocytes” suppresses fatty infiltration and muscle atrophy. Hypothesis: Parathyroid hormone (PTH) suppresses fatty infiltration and muscle atrophy after rotator cuff tears in a rat model by browning of FAPs. Study Design: Controlled laboratory study. Methods: PTH was administered subcutaneously for 4 or 8 weeks to a rotator cuff tear model in rats. After treatment, fatty infiltration of supraspinatus muscles was assessed using Oil Red O staining and muscle atrophy using wet muscle weight and muscle fiber cross-sectional area. Costaining of platelet-derived growth factor receptor α (FAP marker) and uncoupling protein 1 (browning marker) was performed to confirm FAP browning by PTH. Mouse-isolated FAPs were cultured with PTH and evaluated for browning-related gene expression and adipogenic differentiation using BODIPY staining. Myogenic differentiation of C2C12 myoblasts was evaluated using coculture of PTH-treated browning FAPs with C2C12. Results: PTH inhibited fatty infiltration after rotator cuff tear at 8 weeks. Rotator cuff wet muscle loss of PTH-treated rats was inhibited at 4 and 8 weeks. Furthermore, PTH-treated rats demonstrated larger myofiber cross-sectional area than did untreated rats at 4 and 8 weeks. Costaining indicated colocalization of platelet-derived growth factor receptor α and uncoupling protein 1 and promoted PTH-induced FAP browning. PTH increased the expression of browning-related genes in FAPs and suppressed fat droplet accumulation in vitro. Coculture with PTH-treated FAPs promoted C2C12 cell differentiation into myotubes. Conclusion: PTH induced FAP-derived beige adipocytes by upregulating browning-related gene expression, and the browning effect of PTH on FAPs inhibited fatty infiltration and muscle atrophy in the rat rotator cuff tear model. PTH might have potential as a therapeutic drug for fatty infiltration and muscle atrophy after rotator cuff tears. Clinical Relevance: PTH may expand treatment options for rotator cuff tears by reducing fatty infiltration and muscle atrophy after rotator cuff tears by browning of FAPs.
Microcrystalline cellulose and by-products from the pulp and paper industry as reinforcing fibres for polybutylene succinate-based composites: A comparative study
Authors: Sousa Francisco JPM , Jesus Carolina F , Góis Joana R , Pereira Nelson AM , Antunes Filipe E 
Published: August 2023

This study focussed on the development of fully biodegradable composites based on polybutylene succinate (PBS) in combination with raw materials from different processing stages of the pulp and paper industry. Sulfite pulp (SP), kraft pulp (KP) and screened sludge residue (SL) from wastewater treatment plants (WWTP) were investigated as reinforcing fibres contributing to a circular economy. We report a comparative study of the physicochemical properties and mechanical performance of PBS composites reinforced with these cellulosic raw fibres and commercial pure microcrystalline cellulose (MC). The composites were characterised in tensile strength, elongation at break, modulus of elasticity, water absorption and wettability. The incorporation of the fibres into the polymer matrix was also investigated using torque rheometry. Critical concentrations were obtained with maximum incorporation in the range of 15–20 wt% for SP/KP and 25–30 wt% for SL. SP and KP composites showed at least as good physicochemical and mechanical properties as MC at the concentrations studied (0–20 wt%). SP-composites showed the highest tensile strength and modulus of elasticity. It was shown that these fibres from the pulp and paper industry can be used as reinforcement for PBS matrices without additional chemical purification and provide a valuable alternative to commercial microcrystalline cellulose.
Efficacy of thymosin-α-1 in patients with COVID-19: A systematic review and meta-analysis
Authors: Wang Pu , Wang Changhong , Chen Da 
Published: August 2023

Objective To identify whether thymosin-α-1 (Tα1) is effective in patients with Coronavirus disease 2019 (COVID-19) and to determine a suitable population for Tα1 treatment. Methods We included studies with ≥10 cases and adults (aged ≥18 years) with laboratory-confirmed SARS-CoV-2 infection, data on mortality or length of hospitalization, disease severity, and study location, while excluded pregnant and breastfeeding women and minors. Publications were searched from November 1, 2019, to July 5, 2023, in six databases, including PubMed, Web of Science, Embase, Cochrane Library, China Knowledge Resource Integrated Database, and Wanfang Database. We separately utilized Newcastle-Ottawa Scale and Cochrane handbook methodology to evaluate risk of bias and used Review Manager (version 5.4, Cochrane Collaboration, Copenhagen, Denmark) to present and synthesize results. Relative risks (RR) and Standardized Mean Difference (SMD) with 95% confidence intervals (CI) were analyzed for dichotomous variables and continuous variables, respectively. Results Nine studies (participants = 5417) were included. No significant differences were found in mortality (nine studies; n = 5417; RR = 0.95; 95% CI: 0.56, −1.60; p = .84; I2 = 90%) or length of hospitalization (four studies; n = 3688; SMD = 0.16; 95% CI: −0.38, −0.69; p = .57; I2 = 96%) between patients with COVID-19 who did and did not receive Tα1. Participants were divided by the severity of the disease (serious and non-serious) and study location. Among the serious group, the incidence of death among patients who received Tα1 treatment was 0.67 times that of patients who did not receive Tα1 treatment (four studies; n = 1230; RR: 0.67; 95% CI: 0.58, −0.77; p < .00,001; I2 = 0%). There was no significant difference in length of hospitalization between the groups (two studies; n = 410; SMD = 0.66; 95% CI: −0.06, −1.38; p = .07; I2 = 87%). Among the non-serious group, compared to not having Tα1 treatment, receiving Tα1 treatment reduced hospitalization length (two studies; n = 3670; SMD = −0.28; 95% CI: −0.41, −0.14; p < .0001; I2 = 51%), while no significant difference in mortality (three studies; n = 3775; RR = 1.06; 95% CI: 0.22, −5.03; p = .94; I2 = 89%). Moreover, there was no significant difference between subgroups when divided by study locations (Studies within China: seven studies; n = 5263; RR = 1.14; 95% CI: 0.64, −2.04; p = .65; I2=92%; Studies outside of China: two studies; n = 154; RR = 0.41; 95% CI: 0.14, −1.24; p = .11; I2 = 51%). Discussion For patients with serious types of COVID-19, Tα1 significantly decreased mortality, which supports the utilization of Tα1 in patients with severe and critical types of COVID-19. Moreover, regarding hospitalization length, patients with non-serious COVID-19 who used Tα1 reduced their hospitalization length compared to those that did not use Tα1. However, these results have high heterogeneity and limited generalizability.
Severe endosalpingiosis: Case reports and a literature review
Authors: Bazmi Subul , Amer Saad 
Published: August 2023

Background: Endosalpingiosis is an understudied gynaecological condition with limited knowledge of its prevalence and clinical significance. Methods: We report two rare cases of severe endosalpingiosis aged 25 and 30 highlighting their clinical, laparoscopic and histological features, as well as treatment outcomes. Results: Both cases presented with severe and intractable chronic pelvic pain (CPP) with a pattern like endometriosis pain. Initially, they received standard medical management for CPP, including hormonal therapy and pain modulators, with limited or no improvement. They were then offered laparoscopy, which revealed widely spread superficial vesicular lesions on ovaries, pelvic peritoneum, uterus and bowel. Wide excision of affected peritoneum was performed, and multiple biopsies were obtained from ovarian lesions. Histology revealed endosalpingiosis in both cases. In case 2, histology also revealed a neuroendocrine tumour, which was likely from a gastrointestinal (GI) primary malignancy. This case was referred to the GI team for further management. In both cases, surgery resulted in 6-month relief of pelvic pain followed by gradual recurrence of severe symptoms. Conclusion: These cases highlight the clinical dilemma of severe endosalpingiosis, which appears to be associated with intractable pain that is resistant to all standard hormonal and surgical treatments. Whether the association with the neuroendocrine tumour in the second case was incidental or a true link remains uncertain. Further research is required to identify effective treatment strategies for ES.
The fate of mitochondria during platelet activation
Authors: Grichine Alexei , Jacob Shancy , Eckly Anita , Villaret Joran , Joubert Clotilde , Appaix Florence , Pezet Mylène , Ribba Anne-Sophie , Denarier Eric , Mazzega Jacques , Rinckel Jean-Yves , Lafanechère Laurence , Elena-Herrmann Bénédicte , Rowley Jesse W. , Sadoul Karin 
Published: August 2023

Abstract
Blood platelets undergo several successive motor-driven reorganizations of the cytoskeleton when they are recruited to an injured part of a vessel. These reorganizations take place during the platelet activation phase, the spreading process on the injured vessel or between fibrin fibers of the forming clot, and during clot retraction. All these steps require a lot of energy, especially the retraction of the clot when platelets develop strong forces similar to those of muscle cells. Platelets can produce energy through glycolysis and mitochondrial respiration. However, although resting platelets have only 5 to 8 individual mitochondria, they produce adenosine triphosphate predominantly via oxidative phosphorylation. Activated, spread platelets show an increase in size compared with resting platelets, and the question arises as to where the few mitochondria are located in these larger platelets. Using expansion microscopy, we show that the number of mitochondria per platelet is increased in spread platelets. Live imaging and focused ion beam–scanning electron microscopy suggest that a mitochondrial fission event takes place during platelet activation. Fission is Drp1 dependent because Drp1-deficient platelets have fused mitochondria. In nucleated cells, mitochondrial fission is associated with a shift to a glycolytic phenotype, and using clot retraction assays, we show that platelets have a more glycolytic energy production during clot retraction and that Drp1-deficient platelets show a defect in clot retraction.
Novel and improved Caenorhabditis briggsae gene models generated by community curation
Authors: Moya Nicolas D. , Stevens Lewis , Miller Isabella R. , Sokol Chloe E. , Galindo Joseph L. , Bardas Alexandra D. , Koh Edward S. H. , Rozenich Justine , Yeo Cassia , Xu Maryanne , Andersen Erik C. 
Published: August 2023

Abstract
Background
The nematode Caenorhabditis briggsae has been used as a model in comparative genomics studies with Caenorhabditis elegans because of their striking morphological and behavioral similarities. However, the potential of C. briggsae for comparative studies is limited by the quality of its genome resources. The genome resources for the C. briggsae laboratory strain AF16 have not been developed to the same extent as C. elegans. The recent publication of a new chromosome-level reference genome for QX1410, a C. briggsae wild strain closely related to AF16, has provided the first step to bridge the gap between C. elegans and C. briggsae genome resources. Currently, the QX1410 gene models consist of software-derived gene predictions that contain numerous errors in their structure and coding sequences. In this study, a team of researchers manually inspected over 21,000 gene models and underlying transcriptomic data to repair software-derived errors.

Results
We designed a detailed workflow to train a team of nine students to manually curate gene models using RNA read alignments. We manually inspected the gene models, proposed corrections to the coding sequences of over 8,000 genes, and modeled thousands of putative isoforms and untranslated regions. We exploited the conservation of protein sequence length between C. briggsae and C. elegans to quantify the improvement in protein-coding gene model quality and showed that manual curation led to substantial improvements in the protein sequence length accuracy of QX1410 genes. Additionally, collinear alignment analysis between the QX1410 and AF16 genomes revealed over 1,800 genes affected by spurious duplications and inversions in the AF16 genome that are now resolved in the QX1410 genome.

Conclusions
Community-based, manual curation using transcriptome data is an effective approach to improve the quality of software-derived protein-coding genes. The detailed protocols provided in this work can be useful for future large-scale manual curation projects in other species. Our manual curation efforts have brought the QX1410 gene models to a comparable level of quality as the extensively curated AF16 gene models. The improved genome resources for C. briggsae provide reliable tools for the study of Caenorhabditis biology and other related nematodes.
Five point initiative: a community-informed bundled implementation strategy to address HIV in Black communities
Authors: Dale Sannisha K. , Etienne Kayla , Hall Sidny , Lazarus Kimberly , Nunnally Kalenthia , Gibson George , Bolden Roxana , Gardner Nadine , Sanders Jasmyn , Reid Rachelle , Phillips Arnetta 
Published: August 2023

Abstract
Background
Black individuals in the U.S. remain the most disproportionately impacted by new HIV diagnoses, represent the highest portion of individuals living with HIV, and have the highest morbidity rates. Structural inequities and historical oppression are the primary drivers. Such drivers limit access to HIV prevention tools that need to be delivered with culturally congruent and community-informed approaches.

Methods
The Five Point Initiative (FPI) is a community-informed bundled implementation strategy developed and piloted between September 2019 and March 2020 in Miami, Florida in communities heavily impacted by HIV. Key components of the strategy included community consultants/experts, five categories (hence the “Five Point”) of community businesses (e.g., corner stores, beauty supply stores, laundromats, mechanics, barbershops), local health organizations, an academic research program engrossed in community engaged research, and community residents who provided ongoing feedback throughout. Outcomes of FPI included (a) survey information (e.g., knowledge of and access to PrEP, barriers to care) and pilot data (acceptability and feasibility), (b) expansion of reach to Black individuals in HIV high impact zip codes in Miami, (c) insights on our bundled implementation strategy, (d) condom distribution, and (e) HIV testing.

Results
Over the course of six months FPI carried out 10 outreach events, partnered with 13 community businesses and 5 health organizations, engaged 677 community residents, collected health information via a survey, distributed 12,434 condoms, provided information on PrEP, and offered voluntary HIV testing (131 completed). FPI’s ability to reach residents who are not being reached (e.g., 68.8% never heard of PrEP, 8% no HIV testing ever, 65.9% no primary care provider), positive feedback from residents (e.g., 70% very satisfied, 21% satisfied; 62% strongly agree and 25% agree they would participate again) and qualitative interviews with businesses provide evidence of acceptability and feasibility. Further, survey data provided insights on factors such as socio-demographics, discrimination experiences, barriers to care, social-structural factors, physical and sexual health, and mental health and substance use.

Conclusions
The FPI bundled implementation strategy shows promise to deliver health prevention/intervention for HIV and other health conditions to communities facing health inequities and for whom the current system for delivering care is insufficient.
Construction of an evaluation index system for undergraduate nursing teachers’ curriculum humanistic competence: a Delphi study in China
Authors: Yang Zihan , Zhai Huimin , Liang Sijing 
Published: August 2023

Abstract
Background
The curriculum humanistic competence of nursing teachers is important to cultivate the humanistic qualities of undergraduate nursing students. However, there are no evaluation tools for the curriculum humanistic competence of undergraduate nursing teachers in China.

Objective
To develop an index system to evaluate the curriculum humanistic competence of undergraduate nursing teachers.

Design
This research conducted a Delphi study.

Participants
Semi-structured interviews were held with 19 experts, and Delphi rounds were conducted with 18 experts.

Settings
This study was conducted in 12 universities and 4 Grade A tertiary hospitals in China.

Methods
A literature review and semi-structured interviews were conducted to develop an initial framework. A two-round Delphi survey was employed to build the index system for undergraduate nursing teachers’ curriculum humanistic competence.

Results
After two rounds of consultation, the index system included 5 first-level indicators, 11 second-level indicators and 41 third-level indicators. The Cr for two rounds of consultation were 0.929 and 0.923, and Kendall’s W was 0.152(P<0.001).

Conclusions
The index system for the evaluation of undergraduate nursing teachers’ curriculum humanistic competence offers guidelines for undergraduate nursing teachers in China. It can be used in practice to develop high humanistic qualities in undergraduate nursing teachers.
Interprofessional communication in a psychiatric outpatient unit – an ethnographic study
Authors: Rudberg Ingela , Olsson Annakarin , Thunborg Charlotta , Salzmann-Erikson Martin 
Published: August 2023

Abstract
Background
Communication in healthcare has been extensively studied, but most research has focused on miscommunication and the importance of communication for patient safety. Previous research on interprofessional communication has mainly focused on relationships between physicians and nurses in non-psychiatric settings. Since communication is one of the core competencies in psychiatric care, more research on interprofessional communication between other clinicians is needed, and should be explored from a broader perspective. This study aimed to explore and describe interprofessional communication in a psychiatric outpatient unit.

Method
During spring 2022, data consisting of over 100 h of fieldwork were collected from observations, formal semi-structured interviews and informal conversations inspired by the focused ethnography method. Data was collected at an outpatient unit in central Sweden, and various clinicians participated in the study. The data analysis was a back-and-forth process between initial codes and emerging themes, but also cyclical as the data analysis process was ongoing and repeated and took place simultaneously with the data collection.

Results
We found that a workplace’s history, clinicians´ workload, responsibilities and hierarchies influence interprofessional communication. The results showed that the prerequisites for interprofessional communication were created through the unit’s code of conduct, clear and engaging leadership, and trust in the ability of the various clinicians to perform new tasks.

Conclusion
Our results indicate that leadership, an involving working style, and an environment where speaking up is encouraged and valued can foster interprofessional communication and respect for each other´s professional roles is key to achieving this. Interprofessional communication between different clinicians is an important part of psychiatric outpatient work, where efficiency, insufficient staffing and long patient queues are commonplace. Research can help shed light on these parts by highlighting aspects influencing communication.