bluuebunny/crossref_metadata_2025_split huggingface dataset:
Tropical Transition of Tropical Storm Kirogi (2012) over the Western North Pacific: Synoptic Analysis and Mesoscale Simulation
Authors: Yanase Wataru , Shimada Udai , Kitabatake Naoko , Tochimoto Eigo 
Published: July 2023

Abstract
Tropical transition (TT) is a cyclogenesis process in which a baroclinic disturbance is transformed into a tropical cyclone. Many studies have analyzed TT events over the North Atlantic. This study assesses TT processes from a possible subtropical cyclone to Tropical Storm Kirogi at a relatively high latitude over the western North Pacific in an environment of enhanced baroclinicity in August 2012. Analyses based on satellite observations, the JRA-55 reanalysis, and a simulation with 2.5-km horizontal grid spacing demonstrate three stages during the TT: the baroclinic, intermediate, and convective stages. Over the baroclinic stage, Kirogi had an asymmetric comma-shaped cloud pattern with convection in the northern and eastern parts of the cyclone. This convection is attributed to quasigeostrophic forcing and frontogenesis associated with advection of warm and moist air. Vorticity locally generated by this convection was advected to the cyclone center by cyclone-relative northerly flow. Kirogi also had a shallow warm-core structure due to the interaction with an upper-level cold trough extending from the midlatitudes. In the intermediate stage, the warm and moist air in the lower troposphere and the cold trough in the upper troposphere wrapped around Kirogi. In the convective stage, Kirogi attained characteristics of a typical tropical cyclone with convection concentrated near the cyclone center and a deep warm-core structure. These results demonstrate that baroclinic processes can directly trigger formation of a tropical storm at relatively high latitudes over the western North Pacific in a similar manner to that over the North Atlantic.

Significance Statement
Tropical cyclogenesis is an important process for early identification of tropical cyclone hazards. Tropical transition is a tropical cyclogenesis process that is triggered by a subtropical or extratropical disturbance. It is unique to relatively high latitudes and has social importance particularly for midlatitude countries. There have been fewer studies on tropical transition over the western North Pacific than over the North Atlantic. This study demonstrates the dynamics of a distinct tropical transition event that led to the formation of Tropical Storm Kirogi (2012) at a relatively high latitude over the western North Pacific.
Screening the Chemical Composition and Bioactivity of Cerasus serrulata Flower Extracts Using Two Extraction Methods
Authors: Zhang Bing , Zhang Wenyun , Luo Jianfang , He Jian , Zheng Xiaomin , Zhu Siyang , Rong Baoshan , Ai Yong , Zhang Lanyue , He Tinggang 
Published: July 2023

Background Cerasus serrulata has an excellent antioxidative, antibacterial, and anti-alopecia effect, but its active ingredients and the underlying mechanism are currently unclear, in addition to the fact that the active products vary with the extraction methods. Aim Therefore, this study aims to clarify the active components and antioxidative property of C. serrulata extracted by two different methods (low-temperature vacuum extraction and ethanol extraction) and explore its potential biofunction. Materials and Methods First, the compositions in extracts were characterized by gas chromatographic mass spectrometry (GC-MS) and liquid chromatography-mass spectrography (LC-MS). Then, the antioxidative activity of both extracts was evaluated using free radical scavenging assay (ABTS) and ORAC. Next, the antibacterial activity of both extracts was measured via a bacteriostatic circle experiment. The anti-alopecia effects of both extracts were studied using a mouse model of alopecia. The hematoxylin and eosin (H&E) staining was used to evaluate hair follicles; vascular endothelial-derived growth factor (VEGF) and interleukin-6 (IL-6) expression in mouse skin were evaluated by immunohistochemistry, while β-catenin expression in tissues was used by immunofluorescence. The expression of FGFR and insulin-like growth factor 1 (IGF-1) in the skin was determined to evaluate using real-time quantitative polymerase chain reaction (RT-qPCR). The levels of DHT and 5α-reductase in the experimental mice were determined by ELISA. Results The GC-MS analysis results showed that the extracts were rich in alkanes and aromatic compounds, specifically benzaldehyde, which was the highest component in the cold vacuum extraction, while pipecolic acid was the main component in the ethanol extract. This indicates that C. serrulata has components that exhibit strong inhibitory ability against the growth of five common bacterial strains, expressing excellent free radical scavenging ability. In addition, the active ingredients in C. serrulata were found to significantly deregulate the protein levels in the skin of androgenetic alopecia (AGA) mice, with the levels of IL-6. They were also able to significantly inhibit VEGF reduction. The β-catenin was being significantly upregulated in skin tissue. In addition, FGFR and IGF-1 gene expression also increased. The 5α-reductase and dihydrotestosterone (DHT) can be reduced in the C. serrulata treatment. Conclusion Our data indicate that C. serrulata extract could be beneficial supplements of natural antioxidative, antibacterial, and anti-alopecia agents.
Orthodontic alignment of ankylosed teeth with aid of surgical luxation: Case series
Authors: Chong Jun Ai , Mah Eng Ching 
Published: July 2023

Background: Identification of tooth ankylosis before comprehensive orthodontic treatment can be challenging. This case series illustrates different presentations of tooth ankylosis, the importance of early diagnosis, the use of surgical luxation to aid orthodontic alignment of ankylosed teeth and its sequelae. Case presentation: All three cases were adolescents: a 14-year-old girl with a highly placed upper left lateral incisor and history of general anaesthesia; a 14-year-old boy with an impacted upper right first premolar and history of dental trauma; and a 13-year-old girl with an infraoccluded upper left central incisor with history of replantation due to avulsion. Iatrogenic malocclusion developed from attempts to align these ankylosed teeth. Subsequently, surgical luxation was performed, and the ankylosed teeth were successfully aligned. However, it was associated with pulp calcification, root resorption and recurrence of ankylosis. Conclusion: Surgical luxation and orthodontic alignment of ankylosed teeth can be considered a useful short-term solution to delay the need of surgical removal and teeth replacement.
Stentgraft Limb Occlusion After Endovascular Aneurysm Repair: Incidence and Risk Factors
Authors: Van Gerwen Anouk , Gallala Sarah , Kerselaers Laura , Aerden Dimitri , Debing Erik 
Published: July 2023

Introduction Stentgraft limb occlusion (SLO) is a potential complication of endovascular aneurysm repair (EVAR). The purposes of this single centre study are to report the incidence of SLO after EVAR and to detect possible risk factors. Methods All patients who underwent EVAR between June 2001 and February 2020 were included in this retrospective study. Demographic data, cardiovascular risk factors, aneurysm characteristics, arterial anatomy, repair strategy, systemic and stentgraft-related complications, and in-hospital and late mortality were collected. Routine follow-up included duplex examination and/or CT angiography at 3 months, 12 months and annually thereafter. Logistic regression analysis was performed to detect predictors for SLO. Results A total of 221 patients (425 stentgraft limbs) were included; of whom 11 patients (5.0%) occluded. Median time to occlusion was 3.3 months and most of the patients presented ischemic signs. Two risk factors for SLO could be identified: symptomatic aneurysm ( P .015, odds ratio 4.62, 95% confidence interval 1.35-15.86) and length of the infrarenal abdominal aortic aneurysm (AAA) ( P .021, odds ratio 1.31, 95% confidence interval 1.04 - 1.64). Conclusion The incidence of SLO after EVAR is low, and most occlusions occur within the first year. Predictors for SLO are the symptomatic aneurysm and the length of the infrarenal AAA. Further research is necessary to pool all predictors and to assess the clinical impact of different follow-up strategies for high-vs low-risk patients.
Delayed Presentation of Displaced Salter-Harris III Distal Tibia Fracture: Does Transfracture Reduction Yield Satisfactory Outcomes?
Authors: Poulos Nicholas , Byrd Jeffrey , Ernst Brady , Metikala Sreenivasulu 
Published: July 2023

A Salter-Harris type III fracture of the distal tibia is a significant injury because of combined physeal damage and intra-articular extension. With a displaced fracture, anatomic physeal alignment and articular congruity are critical to minimize the risk of complications, but a preoperative delay makes the treatment challenging. Herein, we present a 12-year-old boy with a delayed presentation of a similar physeal injury managed by an open approach using a transfracture reduction technique. We have observed anatomic fracture healing and a favorable outcome with no signs of physeal arrest at a 2-year follow-up. Levels of Evidence: Level V
Prognosis of Cervical Diffuse Idiopathic Skeletal Hyperostosis-Related Spine Injuries in Elderly Patients: Analyses of Both Fracture and Spinal Cord Injury Without Fracture
Authors: Segi Naoki , Nakashima Hiroaki , Machino Masaaki , Ito Sadayuki , Yokogawa Noriaki , Sasagawa Takeshi , Funayama Toru , Eto Fumihiko , Yamaji Akihiro , Watanabe Kota , Nori Satoshi , Takeda Kazuki , Furuya Takeo , Yunde Atsushi , Nakajima Hideaki , Yamada Tomohiro , Hasegawa Tomohiko , Terashima Yoshinori , Hirota Ryosuke , Suzuki Hidenori , Imajo Yasuaki , Ikegami Shota , Uehara Masashi , Tonomura Hitoshi , Sakata Munehiro , Hashimoto Ko , Onoda Yoshito , Kawaguchi Kenichi , Haruta Yohei , Suzuki Nobuyuki , Kato Kenji , Uei Hiroshi , Sawada Hirokatsu , Nakanishi Kazuo , Misaki Kosuke , Terai Hidetomi , Tamai Koji , Shirasawa Eiki , Inoue Gen , Kakutani Kenichiro , Kakiuchi Yuji , Iizuka Yoichi , Takasawa Eiji , Akeda Koji , Takegami Norihiko , Kiyasu Katsuhito , Tominaga Hiroyuki , Tokumoto Hiroto , Funao Haruki , Oshima Yasushi , Yoshii Toshitaka , Kaito Takashi , Sakai Daisuke , Ohba Tetsuro , Seki Shoji , Otsuki Bungo , Ishihara Masayuki , Miyazaki Masashi , Okada Seiji , Imagama Shiro , Kato Satoshi 
Published: July 2023

Study Design Retrospective multicenter study. Objective The purpose of this study was to compare the prognosis of elderly patients with injuries related to cervical diffuse idiopathic skeletal hyperostosis (cDISH) to matched control for each group, with and without fractures. Methods The current multicenter study was a retrospective analysis of 140 patients aged 65 years or older with cDISH-related cervical spine injuries; 106 fractures and 34 spinal cord injuries without fracture were identified. Propensity score–matched cohorts from 1363 patients without cDISH were generated and compared. Logistic regression analysis was performed to determine the risk of early mortality for patients with cDISH-related injury. Results Patients with cDISH-related injuries with fracture did not differ significantly in the incidence of each complication and ambulation or severity of paralysis compared to matched controls. In patients with cDISH-related injury without fracture, those who were nonambulatory at discharge comprised 55% vs 34% of controls, indicating significantly poorer ambulation in those with cDISH-related injuries ( P = .023). There was no significant difference in the incidence of complications and ambulation or paralysis severity at 6 months as compared with controls. Fourteen patients died within 3 months. Logistic regression analysis identified complete paralysis (odds ratio [OR] 36.99) and age (OR 1.24) as significant risk factors for mortality. Conclusions The current study showed no significant differences in the incidence of complications, ambulation outcomes between patients with cDISH-related injury with fracture and matched controls, and that the ambulation at discharge for patients with cDISH-related injury without fractures were significantly inferior to those of matched controls.
Joint fluid interleukin-6 combined with the neutral polymorphonuclear leukocyte ratio (PMN%) as a diagnostic index for chronic periprosthesis infection after arthroplasty
Authors: Su Xudong , Zhu Bo , Qin Leilei , Yang Jianye , Wei Li , Xu Zhenghao , Wei Keyu , Wang Jiayi , Chen Li , Zhao Chen , Chen Cheng , Huang Wei , Xiong Yan , Hu Ning 
Published: July 2023

Abstract
Background
The diagnosis of periprosthetic joint infection (PJI) remains a challenge in clinical practice. Many novel serum and joint fluid biomarkers have important implications for the diagnosis of PJI. The presented study evaluated the value of joint fluid interleukin-6 (IL-6) combined with the neutral polymorphonuclear leukocyte (PMN%) ratio for chronic PJI diagnosis after arthroplasty.

Materials and methods
Sixty patients with chronic PJI or aseptic failure who underwent hip or knee revision from January 2018 to January 2020 in our department were included in this retrospective study. According to the 2013 MSIS diagnostic criteria, the 60 patients were divided into a PJI group and a non-PJI group (30 patients per group). We collected the joint fluid before surgery and determined the level of IL-6 and the PMN% by ELISA, and the differences between the two groups were compared. The diagnostic efficacy of joint fluid IL-6 combined with PMN% in chronic PJI was analyzed using a receiver operating characteristic curve (ROC curve).

Results
The diagnosis of PJI using joint fluid IL-6 combined with PMN% presented an area under the curve of 0.983, which was more accurate than the areas under the curve for diagnosis using IL-6 and PMN% individually (0.901 and 0.914, respectively). The optimal threshold values for IL-6 and PMN% were 662.50 pg/ml and 51.09%, respectively. Their sensitivity and specificity were 96.67% and 93.33%, respectively. The accuracy of the diagnosis of PJI was 95.00%.

Conclusions
Joint fluid IL-6 combined with PMN% can be used as an auxiliary method to detect chronic infection around the prosthesis after hip/knee arthroplasty.

Level of evidence
Patients who underwent hip/knee revision at the First Hospital of Chongqing Medical University for periprosthetic infection or aseptic failure of the prosthesis after hip/knee arthroplasty from January 2018 to January 2020 were included.
Trial registration This study was approved by the ethics committee of the First Hospital of Chongqing Medical University on September 26, 2018 (local ethics committee number: 20187101) and registered with the China Clinical Trials Registry (registration number: ChiCTR1800020440) with an approval date of December 29, 2018.
Gene expression profiling unveils the temporal dynamics of CIGB-300-regulated transcriptome in AML cell lines
Authors: Vázquez-Blomquist Dania , Ramón Ailyn C. , Rosales Mauro , Pérez George V. , Rosales Ailenis , Palenzuela Daniel , Perera Yasser , Perea Silvio E. 
Published: July 2023

Abstract
Background
Protein kinase CK2 activity is implicated in the pathogenesis of various hematological malignancies like Acute Myeloid Leukemia (AML) that remains challenging concerning treatment. This kinase has emerged as an attractive molecular target in therapeutic. Antitumoral peptide CIGB-300 blocks CK2 phospho-acceptor sites on their substrates but it also binds to CK2α catalytic subunit. Previous proteomic and phosphoproteomic experiments showed molecular and cellular processes with relevance for the peptide action in diverse AML backgrounds but earlier transcriptional level events might also support the CIGB-300 anti-leukemic effect. Here we used a Clariom S HT assay for gene expression profiling to study the molecular events supporting the anti-leukemic effect of CIGB-300 peptide on HL-60 and OCI-AML3 cell lines.

Results
We found 183 and 802 genes appeared significantly modulated in HL-60 cells at 30 min and 3 h of incubation with CIGB-300 for p < 0.01 and FC >  = │1.5│, respectively; while 221 and 332 genes appeared modulated in OCI-AML3 cells. Importantly, functional enrichment analysis evidenced that genes and transcription factors related to apoptosis, cell cycle, leukocyte differentiation, signaling by cytokines/interleukins, and NF-kB, TNF signaling pathways were significantly represented in AML cells transcriptomic profiles. The influence of CIGB-300 on these biological processes and pathways is dependent on the cellular background, in the first place, and treatment duration. Of note, the impact of the peptide on NF-kB signaling was corroborated by the quantification of selected NF-kB target genes, as well as the measurement of p50 binding activity and soluble TNF-α induction. Quantification of CSF1/M-CSF and CDKN1A/P21 by qPCR supports peptide effects on differentiation and cell cycle.

Conclusions
We explored for the first time the temporal dynamics of the gene expression profile regulated by CIGB-300 which, along with the antiproliferative mechanism, can stimulate immune responses by increasing immunomodulatory cytokines. We provided fresh molecular clues concerning the antiproliferative effect of CIGB-300 in two relevant AML backgrounds.
Application of left ventricular endomyocardial biopsy in the diagnosis of mitochondrial cardiomyopathy: a case report
Authors: Fang Chuangsen , Lan Ming 
Published: July 2023

Abstract
Background
The clinical features of mitochondrial cardiomyopathy (MCM) are diverse. It can present as hypertrophic cardiomyopathy or dilated cardiomyopathy. The diagnosis of MCM is challenging and usually based on biopsy.

Case presentation
The 30-year-old man was admitted to hospital due to dyspnea for 1 month and edema of both lower extremities for 1 week. Echocardiography suggested a whole heart enlargement, a whole heart diminished function. Renal impairment and diabetes were observed. Coronary angiography showed single-vessel disease (90% stenosis in the ostium of a small marginal branch). Left ventricular endomyocardial biopsy was performed.

Conclusion
Myocardial histopathology demonstrated a large number of abnormal mitochondrial accumulation, so the diagnosis was considered as mitochondrial cardiomyopathy.
Experience of a TelEmergency program in Colombia South America: descriptive observational study between 2019 and 2021
Authors: Vallejo-Bocanumen Carlos E. , Pérez-Martínez Daniel , Quiceno-Salazar Diana Carolina , Mejía-Gonzalez Yésica Paola , García-Cano Juan F. , Martínez-Pérez Diana C. 
Published: July 2023

AbstractIntroductionColombia has 50,912,429 inhabitants, but only 50–70% of the population can effectively access health care services. The emergency room (ER) is a main contributor to the in-hospital care system since up to half of the admissions come through it. Telemedicine has become a tool to facilitate effective access to health care services, improve the timeliness of care, reduce diagnostic variability, and reduce costs associated with health. The aim of this study is to describe the experience of a Distance Emergency Care Program through Telemedicine (TelEmergency) to improve specialist access for patients at the Emergency Room (ER) in low- and medium-level care hospitals in Colombia.MethodsAn observational descriptive study of a cohort including 1,544 patients during the program’s first two years was conducted. Descriptive statistics were used to analyze the available data. The data are presented with summarized statistics of sociodemographic, clinical, and patient-care variables.ResultsThe study included a total of 1,544 patients, and the majority were adults between 60 and 79 years of age (n = 491, 32%). More than half were men (n = 832, 54%), and 68% (n = 1,057) belonged to the contributory health care regime. The service was requested from 346 municipalities, 70% (n = 1,076) from intermediate and rural settings. The most common diagnoses were related to COVID-19 (n = 356, 22%), respiratory diseases (n = 217, 14%), and cardiovascular diseases (n = 162, 10%). We observed 44% (n = 681) of local admissions either under observation (n = 53, 3%) or hospitalization (n = 380, 24%), limiting the need for hospital transfers.Program operation data revealed that 50% (n = 799) of requests were answered within two hours by the medical staff. The initial diagnosis was modified in 7% (n = 119) of the patients after being evaluated by specialists at the TelEmergency program.ConclusionsThis study shows the operational data collected during the first two years after the implementation of the TelEmergency program in Colombia, the first of its kind in the country. Its implementation offered specialized timely management of patients at the ER in low- and medium-level care hospitals, where there is no availability of specialized doctors.
Lewy body disease as a potential negative outcome modifier of glioblastoma treatment: a case report
Authors: Wong Eric T. , Rosenberg Harry , Dawood Olivia , Hertan Lauren , Vega Rafael A. , Anderson Matthew , Uhlmann Erik J. 
Published: July 2023

AbstractBackgroundElderly patients with glioblastoma are particularly susceptible to the adverse effects of ionizing radiation to the brain. This population also has an increasing prevalence of dementia in the successive seventh, eighth and nineth decade of life, and dementia with Lewy bodies is characterized by pathologic α-synucleins, proteins that take part in neuronal DNA damage repair.Case presentationWe report a 77-year-old man, with a history of coronary artery disease and mild cognitive impairment, who experienced subacute behavioral changes over 3 months with wording-finding difficulty, memory loss, confusion, perseveration, and irritable mood. Neuroimaging studies disclosed a 2.5 × 2.4 × 2.7 cm cystic enhancing mass with central necrosis in the left temporal lobe of the brain. Gross total resection of the tumor revealed IDH-1 wild-type glioblastoma. After treatment with radiation and temozolomide chemotherapy, his cognitive status deteriorated rapidly, and he died from unexpected sudden death 2 months after radiation. Autopsy of his brain revealed (i) tumor cells with atypical nuclei and small lymphocytes, (ii) neuronal cytoplasmic inclusions and Lewy bodies that were positive for α-synuclein in the midbrain, pons, amygdala, putamen and globus pallidus, and (iii) no amyloid plaques and only rare neurofibrillary tangles near the hippocampi.ConclusionsThis patient most likely had pre-clinical limbic subtype of dementia with Lewy bodies prior to his diagnosis of glioblastoma. The radiation and temozolomide that was used to treat his tumor may have accelerated neuronal damage due to induction of DNA breakage when his brain was already compromised by pathologic α-synucleins. α-Synucleinopathy could be a negative outcome modifier in glioblastoma patients.