bluuebunny/crossref_metadata_2025_split huggingface dataset:
Six years’ experience and trends of serum 25-hydroxy vitamin D concentration and the effect of vitamin D3 consumption on these trends
Authors: Horváth László , Mirani Sara , Girgis Michael Magdy Fahmy , Rácz Szilvia , Bácskay Ildikó , Bhattoa Harjit Pal , Tóth Béla E. 
Published: July 2023

Introduction: Vitamin D (vitD) deficiency may have importance in some diseases, but there is a lack of data in our country to clarify the current situation. Our aim was to examine the basic characteristics of patients’ vitD status, and the ratio of vitD deficiency and its relation to certain diseases, assess seasonality and trends, and reveal the indirect impact of the COVID-19 pandemic on vitD3 supplementation at the patient population level.Methods: Anonymized data on 25(OH)D test results were obtained from the clinical data registry of a tertiary teaching hospital covering the period between 1 January 2015 and 30 June 2021. VitD consumption (pharmacy sale) data were retrieved from the database of the National Health Insurance Fund of Hungary in order to calculate the defined daily dose (DDD)/1,000 inhabitants/day. Descriptive statistics and odds ratios with their 95% confidence intervals were calculated. The two-sample t-test and F-test were used to analyze our patients’ data. Significant differences were considered if p &lt;0.05.Results: Altogether, 45,567 samples were investigated; the mean age was 49 ± 19.1 years and 68.4% of them were female subjects. Overall, 20% of all patients had hypovitaminosis D, and just over 7% of patients had vitD deficiency. Male subjects had higher odds for hypovitaminosis or vitD deficiency (65.4 ± 28.2 nmol/L vs. 68.4 ± 28.4 nmol/L; p &lt;0.0001). The mean 25(OH)D concentration has changed during the year, reaching a peak in September and a minimum in February. Patients with diseases of the circulatory system, genitourinary system, certain conditions originating in the perinatal period, and “sine morbo” (i.e., without a disease; such as those aged over 45 years and female teenagers) had statistically higher odds for lower 25(OH)D concentrations (p &lt;0.00001). VitD consumption showed seasonality, being higher in autumn and winter. A slight increase started in the season of 2017/18, and two huge peaks were detected at the beginning of 2020 and 2021 in association with the COVID-19 waves.Conclusion: Our data are the first to describe data concerning vitD in our region. It reinforces the notion of vitD3 supplementation for some risk groups and also in healthy individuals. To prevent the winter decline, vitD3 supplementation should be started in September. This and the results during the COVID-19 pandemic highlight the importance of health education encouraging vitamin D3 supplementation.
Abnormal balance control mechanisms during dynamic reaching forward and quiet standing in patients with anterior cruciate ligament reconstruction
Authors: Wang Wei , Li Xudong , Shi Runxiu , Wang Cheng , Zhang Ke , Ren Xiaomin , Wei Hui 
Published: July 2023

Purpose: Postural instability and decreased balance control ability have been observed in patients after anterior cruciate ligament (ACL) reconstruction. Herein, we examined the abnormal balance control mechanisms of these patients during dynamic reaching forward and quiet standing, providing a quantitative index for rehabilitation assessment.Methods: We enrolled ACL reconstruction patients 6–8 months after surgery, and 14 gender- and age-matched healthy volunteers. The IKDC and Lysholm were applied in each patient after ACL reconstruction. All participants conducted the quiet standing and reaching forward (RF) tests at the specified locations on force plates. The ground reaction force, center of pressure (COP), and kinematics signals were recorded. The maximal reach distance (MRD), speed of RF, length of COP, peak speed of COP in anterior-posterior direction (AP-COP), and weight bearing ratio (WBR) of the affected limb were calculated in the RF test. The COP speed, COP amplitude, frequency components of COP and WBR were extracted during quiet standing.Results: We observed that the speed of RF in the patients after ACL reconstruction was significantly lower than that of controls (p &lt; 0.05). The COP length during RF was positively correlated with the Lysholm scale in the affected limb of patients (r = 0.604, p &lt; 0.05). The peak of AP-COP speed during RF in the affected limb of patients was significantly lower than that of the healthy controls (p &lt; 0.05), and positively correlated with the IKDC scale (r = 0.651, p &lt; 0.05). WBR on the affected limb of patients during RF were significantly lower than that of controls (p &lt; 0.05). The mean (r = −0.633, p &lt; 0.05) and peak (r = −0.643, p &lt; 0.05) speeds of COP during quiet standing were negatively correlated with the IKDC scale value. The amplitude of AP-COP on the contralateral side of patients was significantly higher than that of controls during quiet standing (p &lt; 0.05).Conclusion: Patients after ACL reconstruction performed decreased postural control capacity, especially in dynamic balance, and were accompanied by deficiencies in proprioception. The COP length, peak speed of COP during RF and COP speed during quiet standing could be considered as quantitative index of balance function assessment after ACL reconstruction.
The influence of a color themed HMI on trust and take-over performance in automated vehicles
Authors: el Jouhri Aboubakr , el Sharkawy Ashraf , Paksoy Hakan , Youssif Omar , He Xiaolin , Kim Soyeon , Happee Riender 
Published: July 2023

IntroductionSAE Level 3 is known as conditional driving automation. As long as certain conditions are met, there is no need to supervise the technology and the driver can engage in non-driving related tasks (NDRTs). However, a human driver must be present and alert to take over when the automation is facing its system limits. When such an emergency takes place, the automation uses the human machine interface (HMI) to send a take-over request (TOR) to the driver.MethodsWe investigated the influence of a color themed HMI on the trust and take-over performance in automated vehicles. Using a driving simulator, we tested 45 participants divided in three groups with a baseline auditory HMI and two advanced color themed HMIs consisting of a display and ambient lighting with the colors red and blue. Trust in automation was assessed using questionnaires while take-over performance was assessed through response time and success rate.ResultsCompared to the baseline HMI, the color themed HMI is more trustworthy, and participants understood their driving tasks better. Results show that the color themed HMI is perceived as more pleasant compared to the baseline HMI and leads to shorter reaction times. Red ambient lighting is seen as more urging than blue, but HMI color did not significantly affect the general HMI perception and TOR performance.DiscussionFurther research can explore the use of color and other modalities to express varying urgency levels and validate findings in complex on road driving conditions.
Comparison of false positive and false negative rates of two indices of individual reliable change: Jacobson-Truax and Hageman-Arrindell methods
Authors: Ferrer-Urbina Rodrigo , Pardo Antonio , Arrindell Willem A. , Puddu-Gallardo Giannina 
Published: July 2023

BackgroundQuantification of change is crucial for correctly estimating the effect of a treatment and for distinguishing random or non-systematic changes from substantive changes. The objective of the present study was to learn about the performance of two distribution-based methods [the Jacobson-Truax Reliable Change Index (RCI) and the Hageman-Arrindell (HA) approach] that were designed for evaluating individual reliable change.MethodsA pre-post design was simulated with the purpose to evaluate the false positive and false negative rates of RCI and HA methods. In this design, a first measurement is obtained before treatment and a second measurement is obtained after treatment, in the same group of subjects.ResultsIn relation to the rate of false positives, only the HA statistic provided acceptable results. Regarding the rate of false negatives, both statistics offered similar results, and both could claim to offer acceptable rates when Ferguson’s stringent criteria were used to define effect sizes as opposed to when the conventional criteria advanced by Cohen were employed.ConclusionSince the HA statistic appeared to be a better option than the RCI statistic, we have developed and presented an Excel macro so that the greater complexity of calculating HA would not represent an obstacle for the non-expert user.
Development and validation of a prediction model for mechanical ventilation based on comorbidities in hospitalized patients with COVID-19
Authors: Zhang Yi , Zhu Yang-Jie , Zhu Dao-Jun , Yu Bo-Yang , Liu Tong-Tong , Wang Lu-Yao , Zhang Lu-Lu 
Published: July 2023

BackgroundTimely recognition of respiratory failure and the need for mechanical ventilation is crucial in managing patients with coronavirus disease 2019 (COVID-19) and reducing hospital mortality rate. A risk stratification tool could assist to avoid clinical deterioration of patients with COVID-19 and optimize allocation of scarce resources. Therefore, we aimed to develop a prediction model for early identification of patients with COVID-19 who may require mechanical ventilation.MethodsWe included patients with COVID-19 hospitalized in United States. Demographic and clinical data were extracted from the records of the Healthcare Cost and Utilization Project State Inpatient Database in 2020. Model construction involved the use of the least absolute shrinkage and selection operator and multivariable logistic regression. The model’s performance was evaluated based on discrimination, calibration, and clinical utility.ResultsThe training set comprised 73,957 patients (5,971 requiring mechanical ventilation), whereas the validation set included 10,428 (887 requiring mechanical ventilation). The prediction model incorporating age, sex, and 11 other comorbidities (deficiency anemias, congestive heart failure, coagulopathy, dementia, diabetes with chronic complications, complicated hypertension, neurological disorders unaffecting movement, obesity, pulmonary circulation disease, severe renal failure, and weight loss) demonstrated moderate discrimination (area under the curve, 0.715; 95% confidence interval, 0.709–0.722), good calibration (Brier score = 0.070, slope = 1, intercept = 0) and a clinical net benefit with a threshold probability ranged from 2 to 34% in the training set. Similar model’s performances were observed in the validation set.ConclusionA robust prognostic model utilizing readily available predictors at hospital admission was developed for the early identification of patients with COVID-19 who may require mechanical ventilation. Application of this model could support clinical decision-making to optimize patient management and resource allocation.
Single chest drain is not inferior to double chest drain after robotic esophagectomy: a propensity score-matched analysis
Authors: Eckert F. , Merboth F. , Giehl-Brown E. , Hasanovic J. , Müssle B. , Plodeck V. , Richter T. , Welsch T. , Kahlert C. , Fritzmann J. , Distler M. , Weitz J. , Kirchberg J. 
Published: July 2023

BackgroundChest drain management has a significant influence on postoperative recovery after robot-assisted minimally invasive esophagectomy (RAMIE). The use of chest drains increases postoperative pain by irritating intercostal nerves and hinders patients from early postoperative mobilization and recovery. To our knowledge, no study has investigated the use of two vs. one intercostal chest drains after RAMIE.MethodsThis retrospective cohort study evaluated patients undergoing elective RAMIE with gastric conduit pull-up and intrathoracic anastomosis. Patients were divided into two groups according to placement of one (11/2020–08/2022) or two (08/2018–11/2020) chest drains. Propensity score matching was performed in a 1:1 ratio, and the incidences of overall and pulmonary complications, drainage-associated re-interventions, radiological diagnostics, analgesic use, and length of hospital stay were compared between single drain and double drain groups.ResultsDuring the study period, 194 patients underwent RAMIE. Twenty-two patients were included after propensity score matching in the single and double chest drain group, respectively. Time until removal of the last chest drain [postoperative day (POD) 6.7 ± 4.4 vs. POD 9.4 ± 2.7, p = 0.004] and intensive care unit stay (4.2 ± 5.1 days vs. 5.3 ± 3.5 days, p = 0.01) were significantly shorter in the single drain group. Overall and pulmonary complications, drainage-associated events, re-interventions, number of diagnostic imaging, analgesic use, and length of hospital stay were comparable between both groups.ConclusionThis study is the first to demonstrate the safety of single intercostal chest drain use and, at least, non-inferiority to double chest drains in terms of perioperative complications after RAMIE.
Contribution of cats and dogs to SARS-CoV-2 transmission in households
Authors: Fischer Egil A. J. , Broens Els M. , Kooistra Hans S. , De Rooij Myrna M. T. , Stegeman Jan Arend , De Jong Mart C. M. 
Published: July 2023

IntroductionSARS-CoV-2 is known to jump across species. The occurrence of transmission in households between humans and companion animals has been shown, but the contribution of companion animals to the overall transmission within a household is unknown. The basic reproduction number (R0) is an important indicator to quantify transmission. For a pathogen with multiple host species, such as SARS-CoV-2, the basic reproduction number needs to be calculated from the partial reproduction numbers for each combination of host species.MethodIn this study, the basic and partial reproduction numbers for SARS-CoV-2 were estimated by reanalyzing a survey of Dutch households with dogs and cats and minimally one SARS-CoV-2-infected human.ResultsFor households with cats, a clear correlation between the number of cats and the basic reproduction number (Spearman's correlation: p 0.40, p-value: 1.4 × 10−5) was identified, while for dogs, the correlation was smaller and not significant (Spearman's correlation: p 0.12, p-value: 0.21). Partial reproduction numbers from cats or dogs to humans were 0.3 (0.0–2.0) and 0.3 (0.0–3.5) and from humans to cats or dogs were 0.6 (0.4–0.8) and 0.6 (0.4–0.9).DiscussionThus, the estimations of within-household transmission indicated the likelihood of transmission from these companion animals to humans and vice versa, but the observational nature of this study limited the ability to establish conclusive evidence. This study's findings support the advice provided during the pandemic to COVID-19 patients to maintain distance from companion animals as a precautionary measure and given the possibility of transmission, although there is an overall relatively limited impact on the pandemic when compared to human-to-human transmission.
Wide-field optical coherence tomography for microstructural analysis of key tissue types: a proof-of-concept evaluation
Authors: Rabindran Beryl , Corben Adriana D. 
Published: July 2023

Introduction: The presence of positive margins following tumor resection is a frequent cause of re-excision surgery. Nondestructive, real-time intraoperative histopathological imaging methods may improve margin status assessment at the time of surgery; optical coherence tomography (OCT) has been identified as a potential solution but has not been tested with the most common tissue types in surgical oncology using a single, standardized platform.Methods: This was a proof-of-concept evaluation of a novel device that employs wide-field OCT (WF-OCT; OTIS 2.0 System) to image tissue specimens. Various cadaveric tissues were obtained from a single autopsy and were imaged with WF-OCT then processed for permanent histology. The quality and resolution of the WF-OCT images were evaluated and compared to histology and with images in previous literature.Results: A total of 30 specimens were collected and tissue-specific microarchitecture consistent with previous literature were identified on both WF-OCT images and histology slides for all specimens, and corresponding sections were correlated. Application of vacuum pressure during scanning did not affect specimen integrity. On average, specimens were scanned at a speed of 10.3 s/cm2 with approximately three features observed per tissue type.Conclusion: The WF-OCT images captured in this study displayed the key features of the most common human tissue types encountered in surgical oncology with utility comparable to histology, confirming the utility of an FDA-cleared imaging platform. With further study, WF-OCT may have the potential to bridge the gap between the immediate information needs of the operating room and the longer timeline inherent to histology workflow.
Friend or Foe? The Endophytic Fungus Alternaria tenuissima Might Be a Major Latent Pathogen Involved in Ginkgo Leaf Blight
Authors: Su Xiaojia , Shi Ruirui , Li Xiaobo , Yu Zine , Hu Linfeng , Hu Haiyan , Zhang Meng , Chang Jingling , Li Chengwei 
Published: July 2023

Ginkgo leaf blight, one of the most economically important ginkgo diseases, has become very prevalent in many places in China. Flavonoids and endophytes are both considered important in ginkgo plant functioning. However, little is known about the potential relationships among ginkgo leaf blight pathogens, flavonoid accumulation profiles in infected leaves, and ginkgo leaf endophytes. In this study, the flavonoid accumulation profiles in infected leaves, pathogens of ginkgo leaf blight, and the endophytes of healthy ginkgo leaves were characterized. The levels of total flavonoids in the healthy parts of the infected leaves were significantly higher than those in the healthy leaves. Furthermore, Alternaria tenuissima, Botryosphaeria dothidea, and Dothiorella gregaria were identified as pathogens of ginkgo leaf blight; among them, A. tenuissima was the major pathogen. The in vitro experiments showed that flavonoids (apigenin, luteolin, and kaempferol) could significantly inhibit the growth of one or more pathogens at a concentration of 10 mg/L. Furthermore, fifty-six ginkgo leaf endophytic fungi (GLEF) from healthy ginkgo plants were isolated and characterized. Among them, Alternaria spp. were the most abundant, and GLEF55 shared the same ITS sequence with the pathogen Alternaria tenuissima. Thereafter, four flavonoid-producing endophytes were selected and their effects on the growth of pathogens were evaluated. The extracts of GLEF55 could significantly inhibit the growth of the pathogens B. dothidea and D. gregaria simultaneously in vitro, but not the growth of the pathogen A. tenuissima. Furthermore, the dual cultures of the candidate GLEF and ginkgo leaf blight pathogens revealed that GLEF55 had a similar growth rate to that of A. tenuissima and D. gregaria, but its growth rate was significantly slower than that of B. dothidea. Finally, the GLEF exhibited variable roles when facing pathogens in ginkgo leaves. Among them, GLEF55 showed similar pathogenicity as the pathogen A. tenuissima when they were dually cultured in ginkgo leaves. By contrast, GLEF17 (an uncultured soil fungus) could significantly counteract the pathogenic effects of A. tenuissima and D. gregaria, but it dramatically exacerbated the pathogenic effects of B. dothidea. Larger lesion areas were observed on the side of ginkgo leaves where GLEF39 (Alternaria sp.) or GLEF54 (Aspergillus ruber) and pathogens were simultaneously inoculated, which suggested that the pathogenicity of specific endophytic fungi occurred when plants were wounded. Overall, A. tenuissima, a major pathogen of ginkgo leaf blight, might lurk inside the plants as a friendly endogenous fungus and convert into a hostilely pathogenic mode at a particular time. This study proposed a possible cause of ginkgo leaf blight and provided potential theoretical guidance for its prevention.
2-DG Regulates Immune Imbalance on the Titanium Surface after Debridement
Authors: Liu Xingchen , Deng Shudan , Xie Jiaxin , Xu Chunxin , Huang Zhuwei , Huang Baoxin , Chen Zhuofan , Chen Shoucheng 
Published: July 2023

Peri-implantitis requires clinical treatments comprised of mechanical and chemical debridement to remove bacterial biofilms. Bone regeneration on the titanium surface after debridement has been a topical issue of peri-implantitis treatments. Increasing evidence has revealed that the immune microenvironment plays a key role in regulating the bone regeneration process. However, it remains unclear what kind of immune microenvironment the titanium surface induces after debridement. In the study, model titanium surface after debridement was prepared via biofilm induction and mechanical and chemical debridement in vitro. Then, the macrophages and naïve CD4+ T lymphocytes were cultured on the titanium surface after debridement for immune microenvironment evaluation, with the original titanium surface as the control. Next, to regulate the immune microenvironment, 2-DG, a glycolysis inhibitor, was further incorporated to regulate macrophages and CD4+ T lymphocytes at the same time. Surface characterization results showed that the bacterial biofilms were completely removed, while the micro-morphology of titanium surface altered after debridement, and the element composition did not change. Compared with the original titanium disc, titanium surface after debridement can lead to the inflammatory differentiation of macrophages and CD4+ T lymphocytes. The percentage of M1 and Th17 inflammatory cells and the expression of their inflammatory factor genes are upregulated. However, 0.3 mmol of 2-DG can significantly reduce the inflammatory differentiation of both macrophages and CD4+ T lymphocytes and inhibit their expression of inflammatory genes. In conclusion, although bacterial biofilms were removed from titanium surface after debridement, the surface topography changes could still induce immune imbalance and form an inflammatory immune microenvironment. However, this inflammatory immune microenvironment can be effectively reversed by 2-DG in vitro, thus creating an immune microenvironment conducive to osteogenesis, which might provide a new perspective for future therapy of peri-implantitis.