bluuebunny/crossref_metadata_2025_split huggingface dataset:
Novel cellular systems unveil mucosal melanoma initiating cells and a role for PI3K/Akt/mTOR pathway in mucosal melanoma fitness.
Authors: Monti Matilde , Gatta Luisa Benerini , Bugatti Mattia , Pezzali Irene , Picinoli Sara , Manfredi Marcello , Lavazza Antonio , Vanella Virginia Vita , De Giorgis Veronica , Zanatta Lucia , Missale Francesco , Zanetti Benedetta , Bozzoni Giovanni , Cadei Moris , Vergani Barbara , Balzarini Piera , Battocchio Simonetta , Facco Carla , Turri-Zanoni Mario , Castelnuovo Paolo , Nicolai Piero , Fonsatti Ester , Leone Biagio Eugenio , Marengo Emilio , Ronca Roberto , Perego Michela , Lombardi Davide , Vermi William 
Published: July 2023

Abstract
Background: Mucosal Melanomas (MM) are highly aggressive neoplasms arising from mucosal melanocytes. Current treatments offer a limited survival benefit for patients with advanced MM; moreover, the lack of pre-clinical cellular systems has significantly limited the understanding of their immunobiology.
Methods: Five novel cell lines were obtained from patient-derived biopsies of MM arising in the sino-nasal mucosa and designated as SN-MM1-5. The morphology, ultrastructure and melanocytic identity of SNMM cell lines were validated by transmission electron microscopy and immunohistochemistry. Moreover, in vivo tumorigenicity of SN-MM1-5 was tested by subcutaneous injection in NOD/SCID mice. Molecular characterization of SN-MM cell lines was performed by a mass-spectrometry proteomic approach, and their sensitivity to PI3K chemical inhibitor LY294002 was validated by Akt activation, measured by pAkt(Ser473) and pAkt(Thr308) in immunoblots, and MTS assay.
Results: This study reports the validation and functional characterization of five newly generated SN-MM cell lines. Compared to the normal counterpart, the proteomic profile of SN-MM is consistent with transformed melanocytes showing a heterogeneous degree of melanocytic differentiation and activation of cancer-related pathways. All SN-MM cell lines resulted tumorigenic in vivo and display recurrent structural variants according to aCGH analysis. Of relevance, the microscopic analysis of the corresponding xenotransplants allowed the identification of clusters of MITF-/CDH1-/CDH2+/ZEB1+/CD271+ cells, supporting the existence of melanoma-initiating cells also in MM, as confirmed in clinical samples. The proteomic analysis of SN-MM cell lines revealed that RICTOR, a subunit of mTORC2 complex, is the most significantly activated upstream regulator, suggesting a relevant role for the PI3K-Akt-mTOR pathway in these neoplasms. Copy number variations involving components of the PI3K/Akt/mTOR pathway were found in SN-MM cell lines. Moreover, phosphorylation of NDRG1 and Akt activation was observed in SN-MM, the latter being constitutive and sustained by PTEN loss in SN-MM2 and SN-MM3. The cell viability impairment induced by LY294002 confirmed a functional role for the PI3K-Akt-mTOR pathway in SN-MM cell lines.
Conclusions: Overall, these novel and unique cellular systems represent relevant experimental tools for a better understanding of the immunobiology of these neoplasms and, as an extension, to MM from other sites.
Quality of Life of Colorectal Cancer Survivors: Gender Differences in Inner Strength and Multiple Identities
Authors: Yu Hannah , Ryu Eunjung 
Published: July 2023

Abstract
Background
This study aimed to investigate the differences in inner strength, multiple identities, and quality of life among colorectal cancer survivors, with a focus on gender. Additionally, the study aimed to identify the factors that influence their quality of life.
Methods
This cross-sectional study included 170 colorectal cancer survivors in Korea. Participants were recruited from an online self-help group for colorectal cancer survivors. Participants were recruited from an online self-help group for survivors of colorectal cancer. Participants were divided into specific groups based on gender for the purpose of examining the differences in study variables, and additional analyses were conducted. The factors influencing their quality of life were identified through multiple regression analysis, without dividing the participants.
Results
The quality of life for male colorectal cancer survivors was higher than that of female survivors. In terms of inner strength, ‘anguish and searching' was higher in male while ‘connectedness' was higher in female. Inner strength was found to be the most powerful influencing factor on quality of life, after controlling for age and gender.
Conclusions
This study aimed to determine the significance of inner strength in survivors of colorectal cancer. It enabled a more comprehensive understanding of the quality of life in colorectal cancer survivors by not only focusing on the factors that impact quality of life, but also by examining how these impacts vary based on gender. To improve the quality of life for survivors of colorectal cancer, it is crucial to understand the impact of factors such as gender and inner strength. Additionally, it is crucial to establish personalized interventions that specifically target their unique characteristics. The inner strength plays an important role in improving the quality of life for survivors of colorectal cancer. This study suggests that inner strength is applicable to male cancer survivors and supports the inner strength theory.
The role of the novel lncRNA lncFABP4 in modulating adipogenic differentiation in buffalo intramuscular preadipocytes
Authors: Li Ruirui , Zhu Ruirui , Yang Xintong , Feng Ye , He Qin , Wang Haopeng , Liu Qingyou , Shi Deshun , Huang Jieping 
Published: July 2023

Abstract
Intramuscular fat (IMF) is a crucial determinant of meat quality and is influenced by various regulatory factors. Despite the growing recognition of the important role of long noncoding RNAs (lncRNAs) in IMF deposition, the mechanisms underlying buffalo IMF deposition remain poorly understood. In this study, we identified and characterized a novel lncRNA, lncFABP4, which was transcribed from the antisense strand of FABP4. Moreover, lncFABP4 significantly increased intramuscular preadipocyte differentiation, as indicated by an increase in the expression of the adipogenic markers peroxisome proliferator-activated receptor gamma (PPARG), CCAAT enhancer binding protein alpha (C/EBPα), and fatty acid binding protein 4 (FABP4). Mechanistically, lncFABP4 was found to have the potential to regulate downstream gene expression by forming transcriptional complexes with the FABP4 protein. These findings contribute to a further understanding of the intricate mechanisms through which lncRNAs modulate intramuscular adipogenesis in buffaloes.
Participatory Design and Evaluation of the “Stem Cells Australia” Website for Delivering Complex Health Knowledge: Mixed Methods Study
Authors: Pang Patrick Cheong-Iao , Munsie Megan , Chang Shanton , Tanner Claire , Walker Christine 
Published: July 2023

Background
The internet has become a commonly used information source for people seeking to understand their health care options. However, inconsistent representation about what stem cell treatments are available and from whom, coupled with the lack of transparency about what has been shown to work or is even safe, can distract and mislead users. Given these challenges, there is a need to develop effective evidence-based tools for delivering information about health care options involving stem cells.

Objective
A need to redesign an existing website in Australia was identified to provide trustworthy information about stem cell research and to counter misinformation. Using a participatory design approach to generate an understanding of what information users need for stem cell treatments, the Stem Cells Australia website serves as a case study for the development and evaluation of websites delivering complex messages about science and health.

Methods
This study comprised 3 steps. First, a focus group and several one-on-one interviews with a purposive sample of users (n=12) were conducted to identify their needs and requirements. Second, we designed a new version of the website based on findings from the focus group and interviews. Finally, for evaluating the participatory design process, we collected 180 days of Google Analytics data for both the original and redesigned versions (90 days for each) and compared their differences using 2-sample z tests.

Results
The feedback from participants was grouped into 3 themes—needed and unwanted information, how and where to obtain information, and their information preferences. These were translated into requirements for rebuilding the website. The redesigned version reached users in other continents, despite the daily numbers of users (−61.2%; P<.001) and sessions (−61.7%; P<.001) decreasing. The redesigned version also showed substantial decrease in daily bounce rate (−97.2%; P<.001), significant increase in the daily average of page reads per session (+110.8%; P<.001), and long daily average for session duration (+22.9%; P=.045). Navigation flow analysis showed more traffic toward web pages related to health conditions in the redesigned version.

Conclusions
Websites about stem cell research need to provide content for vulnerable global audiences. Participatory design that addresses knowledge gaps and information needs can produce better performance and engagement, which can be evaluated using Google Analytics, a common web analytics tool used by many websites. Learnings for improving the metrics regarding website identity, research updates, and clinical trials are concluded, which can inform the future design of websites seeking to engage users and provide reliable and accessible science and health information including but not limited to stem cell research and therapies.
The Impact of Production Factor and Costs on Business Income of Red Onion Farming Business as an Icon of Fried Red Onion Production in Sigi District, Central Sulawesi
Authors: Muis Armin , Lutfi Muhtar , Jokolelono Eko , Nurnaningsih Nurnaningsih , Rafika Ika , Haprin Nuryana Dj 
Published: July 2023

This study aims to determine and analyze direct correlation of capital, man power, land size, and also production costs to income of red onion farming business; the impact of capital, man power, and also land size towards cost of red onion farming business; and the impact of capital, man power, land area towards income through the cost of red onion farming business in Sigi District. This research used primary data. Population was red onion farmer from three villages and the sample was deliberately determined considering that three villages are red onion producing areas in this district. Analysis for this research are using a structural equation model. From the analysis showed: (1) Production factors of capital, man power and land size had a direct and influential impact towards income; (2) Concerning the impact from production factor on costs, the capital variable had a positive and influential impact towards costs; man power variable had a positive and influential impact towards costs, and land size had a positive and influential impact towards costs; (3) In terms of indirect impact, the variables of capital, man power and land size have a positive and influential impact towards income through mediation of red onion farming costs at Sigi District, Central Sulawesi, Indonesia.
Migrations in Sociological Perspective
Authors: Palczewska Milena 
Published: July 2023

Abstract
The phenomenon of migration can be observed from the times of the formation of larger population centers associated with a given area. It can be said that migrations have accompanied the entire history of mankind inseparably. Migration is caused by economic crises (economic migrations), political crises (political migrations) or are triggered militarily as part of “demographic weapon”. Migration should be considered in many aspects - both in terms of security as well as socio-economic. It affects many sectors of the functioning of the state. High mobility of the population contributes to the reduction of social and economic disparities between regions. It is also very beneficial from the economic point of view and contributes to the reduction of unemployment with a distinct regional structure. There are also so-called chain migrations. It turns out that migrants from a given country, region or locality tend to concentrate in the same places in the countries of migration destination. This is due to the access to information among migrants and the need to ensure a sense of security by living in one community. Contemporary migration is carried out practically from all countries of the world. A significant improvement in the mobility of the world’s population contributes to the intensification of this phenomenon. The processes of migration is not connected only to changing the place of residence, but also include changing the affiliation and adaptation of the individual to the community.
Postural stability and plantar pressure parameters in healthy subjects: variability, correlation analysis and differences under open and closed eye conditions
Authors: De Blasiis P. , Caravaggi P. , Fullin A. , Leardini A. , Lucariello A. , Perna A. , Guerra G. , De Luca A. 
Published: July 2023

Introduction: The “postural control system” acts through biomechanical strategies and functional neuromuscular adaptations to maintain body balance under static and dynamic conditions. Postural stability and body weight distribution can be affected by external sensory inputs, such as different visual stimuli. Little information is available about the influence of visual receptors on stabilometric and plantar pressure parameters. The aim of this study was to analyze variability, correlations, and changes in these parameters under open- (OE) and closed-eye (CE) conditions.Methods: A total of 31 stabilometric and plantar pressure parameters were acquired in 20 young and healthy adults during baropodometric examination performed in bipedal standing under both visual conditions. Variability of parameters was evaluated via the coefficient of variation, correlation analysis via Pearson’s R2, and statistical differences via the Wilcoxon test.Results: High intra-subject repeatability was found for all plantar pressure parameters and CoP-speed (CV &lt; 40%) under OE and CE conditions, while CoP-sway area (CoPsa) and length surface function (LSF) showed larger variability (CV &gt; 50%). Mean and peak pressures at midfoot and total foot loads showed the least number of significant correlations with other parameters under both visual conditions, whereas the arch-index and rearfoot loads showed the largest number of significant correlations. The limb side significantly affected most plantar pressure parameters. A trend of larger LSF and lower CoPsa and mean and peak pressures at the right forefoot was found under the CE condition.Discussion: The present study provides a deeper insight into the associations between postural stability and foot load. Interesting postural adaptations, particularly with respect to different visual stimuli, the effect of the dominant side, and the specific role of the midfoot in balance control were highlighted.
ЭФФЕКТИВНОСТЬ ПОСЛЕУБОРОЧНОГО ПРИМЕНЕНИЕ БИОЛОГИЧЕСКОГО ПРЕПАРАТА НА ОСНОВЕ ДРОЖЖЕЙ METSCHNIKOWIA PULCHERRIMA ДЛЯ ОБЕСПЕЧЕНИЯ ДЛИТЕЛЬНОГО ХРАНЕНИЯ И ВЫСОКОГО КАЧЕСТВА ПЛОДОВ
Authors: Аскарова Молдир , Уразова Майра , Корабаева Сауле , Скак Сабина , Туруспекова Сабина 
Published: July 2023

Эффективная защита плодов от поражения при хранении достигается путем проведения защитных мероприятий в течении вегетации, особенное значение имеют обработки в послеуборочный период.
В статье представлены результаты оценки эффективности биологического препарата «Биоконсерванта МР-3» на основе дрожжей (Metschnikowia pulcherrima) для контроля комплекса гнилей плодов яблони, развивающихся при хранении. Объектами исследований являются болезни инфекционного и не инфекционного происхождения, влияющие на длительность сроков хранения плодов. Были взяты три сорта: «Апорт», «Восход», «Талгарское» для проведения наблюдения за лежкостью плодов и степенью их поражения различными болезнями при хранении. Хранение осуществлялось при температуре +1-2°С и относительной влажности 90-95%. Проводились работы по уточнению видового состава болезней, определению естественной убыли массы плодов, изменения твердости мякоти, были проведены биохимические анализы. Твердость мякоти плода измеряли измерителем (penetrometer). Перед закладкой на хранение плоды были обработаны биологическим препаратом Биоконсервант МР-3 в двух формах жидкой и сухой, обработка проводилась путем замачивания плодов в биологическом препарате на 30 секунд, норма расхода препарата Биоконсервант МР-3 (сухой формы -100 г/10 л/воды) и (жидкой формы 100 мл/10 л/воды) кроме контрольного варианта, плоды были заложены в холодильник для проведения дальнейшего наблюдения.
Diderot et Pirandello : les paradoxes du théâtre
Authors: Piccione Caterina 
Published: July 2023

Nombreuses sont les correspondances entre les écrits de Denis Diderot et le théâtre de Luigi Pirandello. Notre intention n’est pas ici de soutenir l’idée d’une anticipation ou d’une influence directe de l’un sur l’autre ; néanmoins, il nous apparaît qu’une étude des similitudes peut mettre en lumière l’intérêt de la théorie diderotienne du comédien et des expérimentations méta-théâtrales.
Co-administration with Tinospora cordifolia attenuates drug induced nephrotoxicity – A histological and biochemical assessment
Authors: Maheshwari V. Vyas Janani , Kumar S. Senthil , Dennis Joseph Leena , P. Kalaivani , R. Siva 
Published: July 2023

Introduction and Aim: Various herbs have been prescribed as a cure for renal disorders by early literature. Nephroprotective herbs are protective against nephrotoxicity. Tinospora cordifolia is known for its role in treating diabetes and disorders of the kidney and metabolism. However, studying its protective effect on drug induced nephrotoxicity at different time periods is wanting. The aim is to study the nephroprotective effect of Tinospora cordifolia on drug induced nephrotoxic changes upon co-administration of the herb with nephrotoxicity induction by the drug.

Materials and Methods: Ethanolic extract of the stem of Tinospora cordifolia was prepared and evaluated for phytochemical constituents. Gentamicin induced nephrotoxicity model in adult, male, Wistar rats was utilised for the study. Gentamicin and the extract of Tinospora cordifolia were co-administered for 8 days. In the kidney, levels of superoxide dismutase, catalase, glutathione peroxidase, reduced glutathione and lipid peroxidation were measured. Assessment of the renal tissue was carried out using histopathological severity grading.

Results: Phytochemicals like glycosides, flavonoids, saponins, steroids, quinone and coumarin were present in the stem of Tinospora cordifolia. Analysing the levels of oxidative stress and antioxidants in the kidney revealed the rise in the level of reduced glutathione (GSH). This correlated with the regenerative changes brought about by the herbal extract on the acute tubular necrosis in the renal tissue induced due to gentamicin nephrotoxicity, at the histological levels in terms of the reduction of enlargement and regeneration of the tubular epithelium.

Conclusion: Tinospora cordifolia extract constituted flavonoids which are known antioxidants. Co-administration of Tinospora cordifolia stem extract is protective in the nephrotoxic condition induced by drugs, hence, shall benefit the kidney when taken along while consuming potentially nephrotoxic drugs.
Evaluation of the diagnostic value and differentiation efficacy of high sensitivity cardiac troponin T2 (hscTnT2) for STEMI and NSTEMI Iraqi patients with acute coronary syndrome
Authors: Abdul Rasool Hussein Ali , Abdulateef Hasan Al-bayati Ali , H. Issa Abdulkareem 
Published: July 2023

Introduction and Aim: Two major clinical forms of acute coronary syndrome (ACS) were categorized; acute myocardial infarction (MI) showing ST-segment elevation (STEMI) with ECG, the other non-ST-segment elevation (NSTEMI). This study was designed using a new generation of high sensitivity cardiac troponin T2 (hscTnT2) in patients with acute coronary syndrome to evaluate the diagnostic value and its efficacy in differentiation of STEMI from NSTEMI.

Materials and Methods: one hundred twenty (120) patients diagnosed to have ACS were included in the study. Apparently, sixty-four (64) healthy subjects were considered as control. ECG and body mass index (BMI) were performed. Blood analysis of levels of glucose, lipid profile and glutamic-oxaloacetic transaminase (GOT) were measured as well as estimated the human hscTnT2 concentrations by enzyme linked immunosorbent assay (ELISA).

Results: ACS patients (STEMI & NSTEMI) showed significant high levels (hscTnT2), Cholesterol, TG, HDL, GOT, FBS and VLDL compared to control. Furthermore, (hscTnT2) serum level in STEMI 225.95 ±120.66 ng/L is significantly higher (P value <0.001) than NSEMI 102.32 ± 58.542 ng/L. the discrimination efficacy of hscTnT2 to differentiating STEMI from NSTEMI is high with cut off value 90.3 ng/L with high sensitivity.

Conclusion: The use of new generations of high sensitivity troponin T aided to reduce the time for accurate diagnosis of acute MI to less than 2 hours. The hscTnT serum value was higher in STEMI than NSTEMI patients and shown to be positively correlated with the degree of cardiac damage in ACS patients. The level of hscTnT can be considered as good discriminating diagnostic biomarkers to differentiate STEMI from NSTEMI patients.
Assessment of the role of zinc finger protein-804a (znf804a) (rs1344706) gene polymorphism in schizophrenia and methamphetamine addiction patients
Authors: Mahir Mohammed Saja , Hassan Hashim Zainab , Sh. Al-Mayah Qasim , Mohammed Hussein Mahir 
Published: July 2023

Introduction and Aim: The zinc finger protein 804A (ZNF804A) gene has garnered significant interest as a gene associated with an increased risk of psychosis. This interest stems from the strong association observed between a specific single nucleotide polymorphism (SNP), namely rs1344706, within the ZNF804A gene, and various psychosis phenotypes, including schizophrenia and bipolar disorder. In this study, we aimed to assess the functional impact of ZNF804A gene polymorphism in individuals diagnosed with schizophrenia and methamphetamine addiction.

Materials and Methods: This nested case control research included 50 individuals with schizophrenia and 50 methamphetamine (crystal) users recruited from Ibn-Rushed Psychiatric Teaching Hospital in Baghdad. 50 healthy subjects were also recruited as a control group. All participants were subjected to thorough physical examination. The serum concentration of Zinc Finger Protein-804A (ZNF804A) was determined using a sandwich enzyme-linked immunosorbent assay. Genomic DNA was isolated from whole blood samples, and the Taqman allelic discrimination method was used to genotype the ZNF804A gene rs1344706 polymorphism.

Results: Patients with schizophrenia had a significantly higher median level of ZNF804A (48.67 ng/L) than controls (25.56 ng/L) or methamphetamine users (35.62 ng/L). The SNP rs1344706 homozygous (AA) genotype was more common in schizophrenia patients (36% vs. 16%) and methamphetamine addiction patients (4%), with significant differences (OR= 1.32, 95%CI= 1.32-11.75, p=0.014 and OR= 11.7, 95%CI=02.43-56.4, p=0.002, respectively).

Conclusion: The AA genotype of rs1344706 polymorphism could be considered a risk factor for schizophrenia but has a protective role against methamphetamine addiction. Serum level of ZNF804A could be used to confirm the diagnosis of schizophrenia in young individuals with subtle clinical features.