bluuebunny/crossref_metadata_2025_split huggingface dataset:
Ivermectin: A Critical Review on Characteristics, Properties, and Analytical Methods
Authors: Suvarna Vasanti 
Published: March 2023

Abstract

Background
Ivermectin is one of the first, safe, broad-spectrum avermectin class of antiparasitic agent, widely used to control parasitic growth in livestock. Ivermectin being highly lipophilic accumulates in fat tissues, causing its long-term existence in the body. Accordingly, ivermectin residues are observed in various animal products such as milk and meat presenting several health hazards. Therefore, monitoring of ivermectin residue levels in the various food products of animal origin is greatly important to ensure the safety of consumers. This review could be of significant importance in the area of ivermectin analytical method development studies.

Objective
In this context, objective of the present work is to provide a critical review of analytical methods in the literature for detection and quantification of ivermectin in pharmaceutical formulations, and biological materials including animal tissues.

Methods
The data from analysis of accessible literature within the time span of 1980 to 2022 was selected.

Results
Several analytical methods based on HPLC–fluorescence detector, UV-diode array detector, HPLC–tandem mass spectrometry (MS/MS), ultra-performance liquid chromatography–MS/MS, and capillary electrophoresis techniques have been utilized for the simultaneous determination of ivermectin singly or in the presence of other drugs with realistic retention times. Several derivatization strategies were used to introduce a fluorophore followed by extraction into organic phase to remove the matrix interferences and enhance the sensitivity by pre-concentration. Methods such as HPLC linked to MS/MS were developed to lower the detection limit and quantification limit, with no requirement for derivatization.

Conclusion
More simple, selective, fast, sensitive, and green chemistry-oriented methods for ivermectin analysis need to be developed. Novel analytical devices based on pulsed electrochemical methods, voltammetry, and amperometry can be developed for real-time analysis of ivermectin, in addition to biosensors based on nanotechnology including quantum dots and nanoparticles, etc.

Highlights
Various methods are described in the review including HPLC techniques with UV or visible spectrophotometric, fluorescence, and mass spectrometric detection, capillary electrophoresis, and immunological methods.
Evaluating Data Fusion Methods to Improve Income Modeling
Authors: Emmenegger Jana , Münnich Ralf , Schaller Jannik 
Published: March 2023

AbstractIncome is an important economic indicator to measure living standards and individual well-being. In Germany, different data sources yield ambiguous evidence for analyzing the income distribution. The Tax Statistics (TS)—an income register recording the total population of more than 40 million taxpayers in Germany for the year 2014—contains the most reliable income information covering the full income distribution. However, it offers only a limited range of socio-demographic variables essential for income analysis. We tackle this challenge by enriching the tax data with information on education and working time from the Microcensus, a representative 1 percent sample of the German population. We examine two types of data fusion methods well suited to the specific data fusion scenario of the TS and the Microcensus: missing-data methods and performant prediction models. We conduct a simulation study and provide an empirical application comparing the proposed data fusion methods, and our results indicate that Multinomial Regression and Random Forest are the most suitable methods for our data fusion scenario.
A multiwavelength study of the IRAS 23139+5939 (G111.256−0.770) star-forming site and its relation to Sh2-157
Authors: Tapia Mauricio , Persi Paolo 
Published: March 2023

ABSTRACTThe far-infrared source IRAS 23139+5939 (G111.25−0.77) is a well-studied massive star-forming region at the centre of a dense molecular clump. It is associated with a low-luminosity and variable methanol maser, a number of water maser clusters, and several compact H ii regions. We present new sub-arcsec-resolution broad- and narrow-band near- and mid-infrared calibrated images. These were analysed together with Wide-field Infrared Survey Explorer images and longer wavelength published data. We establish that IRAS 23139+5939 is a multiple massive young stellar object (YSO) with a spectral energy distribution characteristic of a Class I object. Its deeply embedded (AV = 21) central YSOs have a total luminosity of 3 × 104 L⊙ and a mass of 16 M⊙. We find several small knots of H2 emission in its vicinity, bordering a large CO outflow driven by the protostar. Their distribution suggests the presence of several YSOs with jets. A young embedded stellar cluster of size ∼40 arcsec (0.65 pc) is found around the massive protostar, with evidence of protoplanetary discs in more than 50 per cent of the likely members. We also report a large arc of H2 emission surrounding a nearby H α-emission star displaying a large infrared excess, with a total luminosity of 1.5 × 103 L⊙. Gaia Data Release 3 and very long baseline interferometry parallaxes indicate a common distance of 3.34 kpc to these two YSOs, a distance similar to that to Sh2-157, a nearby massive star-forming region, which appears connected to IRAS 23139+5939 by a warm dust filament.
Constellations of co-orbital planets: horseshoe dynamics, long-term stability, transit timing variations, and potential as SETI beacons
Authors: Raymond Sean N , Veras Dimitri , Clement Matthew S , Izidoro Andre , Kipping David , Meadows Victoria 
Published: March 2023

ABSTRACT
Co-orbital systems contain two or more bodies sharing the same orbit around a planet or star. The best-known flavours of co-orbital systems are tadpoles (in which two bodies’ angular separations oscillate about the L4/L5 Lagrange points 60° apart) and horseshoes (with two bodies periodically exchanging orbital energy to trace out a horseshoe shape in a co-rotating frame). Here, we use N-body simulations to explore the parameter space of many-planet horseshoe systems. We show that up to 24 equal-mass, Earth-mass planets can share the same orbit at 1 au, following a complex pattern in which neighbouring planets undergo horseshoe oscillations. We explore the dynamics of horseshoe constellations, and show that they can remain stable for billions of years and even persist through their stars’ post-main sequence evolution. With sufficient observations, they can be identified through their large-amplitude, correlated transit timing variations. Given their longevity and exotic orbital architectures, horseshoe constellations may represent potential SETI beacons.
Characterising SMSS J2157–3602, the most luminous known quasar, with accretion disc models
Authors: Lai (赖民希) Samuel , Wolf Christian , Onken Christopher A , Bian (边福彦) Fuyan 
Published: March 2023

ABSTRACT
We develop an accretion disc (AD)-fitting method, utilizing thin and slim disc models and Bayesian inference with the Markov-Chain Monte Carlo approach, testing it on the most luminous known quasar, SMSS J215728.21-360215.1, at redshift z = 4.692. With a spectral energy distribution constructed from near-infrared spectra and broad-band photometry, the AD models find a black hole mass of $\log (M_{\rm {AD}}/{\rm M}_{\odot }) = 10.31^{+0.17}_{-0.14}$ with an anisotropy-corrected bolometric luminosity of $\log {(L_{\rm {bol}}/\rm {erg\, s^{-1}})} = 47.87 \pm 0.10$ and derive an Eddington ratio of $0.29^{+0.11}_{-0.10}$ as well as a radiative efficiency of $0.09^{+0.05}_{-0.03}$. Using the near-infrared spectra, we estimate the single-epoch virial black hole mass estimate to be log (MSE/M⊙) = 10.33 ± 0.08, with a monochromatic luminosity at 3000 Å of $\log {(L(\rm {3000{\AA}})/\rm {erg\, s^{-1}})} = 47.66 \pm 0.01$. As an independent approach, AD fitting has the potential to complement the single-epoch virial mass method in obtaining stronger constraints on properties of massive quasar black holes across a wide range of redshifts.
The r-modes of slowly rotating, stratified neutron stars
Authors: Gittins Fabian , Andersson Nils 
Published: March 2023

ABSTRACT
The only r-modes that exist in a globally barotropic, rotating, Newtonian star are the fundamental l = |m| solutions, where l and m are the indices of the spherical harmonic $Y_l^m$ that describe the mode’s angular dependence. This is in stark contrast to a stellar model that is non-barotropic throughout its interior, which hosts all the l ≥ |m| perturbations including radial overtones. In reality, neutron stars are stratified with locally barotropic regions. Therefore, we explore how stratification alters a star’s ability to support r-modes. We consider the globally stratified case and examine the behaviour of the modes as the star gets close to barotropicity. In this limit, we find that all but the fundamental l = |m| perturbations change character and become generic inertial modes. Restricting the analysis to l = |m| perturbations, we develop the r-mode equations in order to consider stellar models that exhibit local barotropicity. Our results for such models show that the r-mode overtones diverge and join the inertial modes. In order to see which r-modes persist and retain their character in realistic neutron stars, these calculations will need to be brought into full general relativity.
Helical coiling of metaphase chromatids
Authors: Kubalová Ivona , Câmara Amanda Souza , Cápal Petr , Beseda Tomáš , Rouillard Jean-Marie , Krause Gina Marie , Holušová Kateřina , Toegelová Helena , Himmelbach Axel , Stein Nils , Houben Andreas , Doležel Jaroslav , Mascher Martin , Šimková Hana , Schubert Veit 
Published: March 2023

AbstractChromatids of mitotic chromosomes were suggested to coil into a helix in early cytological studies and this assumption was recently supported by chromosome conformation capture (3C) sequencing. Still, direct differential visualization of a condensed chromatin fibre confirming the helical model was lacking. Here, we combined Hi-C analysis of purified metaphase chromosomes, biopolymer modelling and spatial structured illumination microscopy of large fluorescently labeled chromosome segments to reveal the chromonema - a helically-wound, 400 nm thick chromatin thread forming barley mitotic chromatids. Chromatin from adjacent turns of the helix intermingles due to the stochastic positioning of chromatin loops inside the chromonema. Helical turn size varies along chromosome length, correlating with chromatin density. Constraints on the observable dimensions of sister chromatid exchanges further supports the helical chromonema model.
Differential requirements for Gcn5 and NuA4 HAT activities in the starvation-induced versus basal transcriptomes
Authors: Zheng Qiaoyun , Qiu Hongfang , Zhang Hongen , Hinnebusch Alan G 
Published: March 2023

Abstract
The histone acetyltransferase (HAT) subunit of coactivator complex SAGA, Gcn5, stimulates eviction of promoter nucleosomes at certain highly expressed yeast genes, including those activated by transcription factor Gcn4 in amino acid-deprived cells; however, the importance of other HAT complexes in this process was poorly understood. Analyzing mutations that disrupt the integrity or activity of HAT complexes NuA4 or NuA3, or HAT Rtt109, revealed that only NuA4 acts on par with Gcn5, and functions additively, in evicting and repositioning promoter nucleosomes and stimulating transcription of starvation-induced genes. NuA4 is generally more important than Gcn5, however, in promoter nucleosome eviction, TBP recruitment, and transcription at most other genes expressed constitutively. NuA4 also predominates over Gcn5 in stimulating TBP recruitment and transcription of genes categorized as principally dependent on the cofactor TFIID versus SAGA, except for the most highly expressed subset including ribosomal protein genes, where Gcn5 contributes strongly to PIC assembly and transcription. Both SAGA and NuA4 are recruited to promoter regions of starvation-induced genes in a manner that might be feedback controlled by their HAT activities. Our findings reveal an intricate interplay between these two HATs in nucleosome eviction, PIC assembly, and transcription that differs between the starvation-induced and basal transcriptomes.
Oncogenic YAP mediates changes in chromatin accessibility and activity that drive cell cycle gene expression and cell migration
Authors: Fetiva Maria Camila , Liss Franziska , Gertzmann Dörthe , Thomas Julius , Gantert Benedikt , Vogl Magdalena , Sira Nataliia , Weinstock Grit , Kneitz Susanne , Ade Carsten P , Gaubatz Stefan 
Published: March 2023

Abstract
YAP, the key protein effector of the Hippo pathway, is a transcriptional co-activator that controls the expression of cell cycle genes, promotes cell growth and proliferation and regulates organ size. YAP modulates gene transcription by binding to distal enhancers, but the mechanisms of gene regulation by YAP-bound enhancers remain poorly understood. Here we show that constitutive active YAP5SA leads to widespread changes in chromatin accessibility in untransformed MCF10A cells. Newly accessible regions include YAP-bound enhancers that mediate activation of cycle genes regulated by the Myb-MuvB (MMB) complex. By CRISPR-interference we identify a role for YAP-bound enhancers in phosphorylation of Pol II at Ser5 at MMB-regulated promoters, extending previously published studies that suggested YAP primarily regulates the pause-release step and transcriptional elongation. YAP5SA also leads to less accessible ‘closed’ chromatin regions, which are not directly YAP-bound but which contain binding motifs for the p53 family of transcription factors. Diminished accessibility at these regions is, at least in part, a consequence of reduced expression and chromatin-binding of the p53 family member ΔNp63 resulting in downregulation of ΔNp63-target genes and promoting YAP-mediated cell migration. In summary, our studies uncover changes in chromatin accessibility and activity that contribute to the oncogenic activities of YAP.
Atlas of phosphoinositide signatures in the retina identifies heterogeneity between cell types
Authors: Rajala Ammaji , Rajala Rahul , Gopinadhan Nair Gopa Kumar , Rajala Raju V S 
Published: March 2023

AbstractPhosphoinositides (PIPs) are a family of minor acidic phospholipids in the cell membrane. Phosphoinositide (PI) kinases and phosphatases can rapidly convert one PIP product into another resulting in the generation of seven distinct PIPs. The retina is a heterogeneous tissue composed of several cell types. In the mammalian genome, around 50 genes encode PI kinases and PI phosphatases; however, there are no studies describing the distribution of these enzymes in the various retinal cell types. Using translating ribosome affinity purification, we have identified the in vivo distribution of PI-converting enzymes from the rod, cone, retinal pigment epithelium (RPE), Müller glia, and retinal ganglion cells, generating a physiological atlas for PI-converting enzyme expression in the retina. The retinal neurons, rods, cones, and RGCs, are characterized by the enrichment of PI-converting enzymes, whereas the Müller glia and RPE are characterized by the depletion of these enzymes. We also found distinct differences between the expression of PI kinases and PI phosphatases in each retinal cell type. Since mutations in PI-converting enzymes are linked to human diseases including retinal diseases, the results of this study will provide a guide for what cell types are likely to be affected by retinal degenerative diseases brought on by changes in PI metabolism.
Do the EEG and behavioral criteria of NREM arousal disorders apply to sexsomnia?
Authors: Rossi Jessica , Gales Ana , Attali Valérie , Leu-Semenescu Smaranda , Dodet Pauline , Groos Elisabeth , Arnulf Isabelle 
Published: March 2023

Abstract

Study Objectives
To establish whether the recent EEG and behavioral criteria of arousal disorders apply to sexsomnia.

Methods
EEG and behavioral markers upon N3 sleep interruptions in videopolysomnography were retrospectively compared in 24 participants with sexsomnia, 41 participants with arousals disorders, and 40 healthy controls. The specificity and sensitivity of previously suggested EEG and behavioral cutoffs for supporting arousal disorders diagnosis were measured in the sexsomnia vs. control groups.

Results
Participants with sexsomnia and arousals disorders showed a higher N3 fragmentation index, slow/mixed N3 arousal index, and number of eye openings during N3 interruptions than healthy controls. Ten (41.7%) participants with sexsomnia (vs. one sleepwalker and no control) displayed an apparently sexual behavior (masturbation, sexual vocalization, pelvic thrusting, and hand within the pajama) during N3 arousal. An N3 sleep fragmentation index ≥6.8/h of N3 sleep and two or more N3 arousals associated with eye opening was 95% specific but poorly (46% and 42%) sensitive for diagnosing sexsomnia. An index of slow/mixed N3 arousals ≥2.5/h of N3 sleep was 73% specific and 67% sensitive. An N3 arousal with trunk raising, sitting, speaking, showing an expression of fear/surprise, shouting, or exhibiting sexual behavior was 100% specific for a diagnosis of sexsomnia.

Conclusions
In patients with sexsomnia, videopolysomnography based markers of arousal disorders are intermediate between healthy individuals and patients with other arousal disorders, supporting the concept of sexsomnia as a specialized, but less neurophysiologically severe, NREM parasomnia. Previously validated criteria for arousal disorders partially fit in patients with sexsomnia.
Validation of the Hierarchical Nature of the AO Spine Sacral Classification and the Development of the Sacral AO Spine Injury Score
Authors: Karamian Brian A. , Schroeder Gregory D. , Lambrechts Mark J. , Canseco Jose A. , Vialle Emiliano N. , Kandziora Frank , Benneker Lorin M. ,   AO Spine Sacral Classification Group Members, Shanmuganathan Rajasekaran , Öner F. Cumhur , Schnake Klaus J. , Kepler Christopher K. , Vaccaro Alexander R. 
Published: March 2023

Study Design:
Global cross-sectional survey.

Objective:
The objective of this study was to validate the hierarchical nature of the AO Spine Sacral Classification System and develop an injury scoring system.

Summary of Background Data:
Although substantial interobserver and intraobserver reliability of the AO Spine Sacral Classification System has been established, the hierarchical nature of the classification has yet to be validated.

Methods:
Respondents numerically graded each variable within the classification system for severity. Based on the results, a Sacral AO Spine Injury Score (AOSIS) was developed.

Results:
A total of 142 responses were received. The classification exhibited a hierarchical Injury Severity Score (ISS) progression (A1: 8 to C3: 95) with few exceptions. Subtypes B1 and B2 fractures showed no significant difference in ISS (B1 43.9 vs. B2 43.4, P=0.362). In addition, the transitions A3→B1 and B3→C0 represent significant decreases in ISS (A3 66.3 vs. B1 43.9, P<0.001; B3 64.2 vs. C0 46.4, P<0.001). Accordingly, A1 injury was assigned a score of 0. A2 and A3 received scores of 1 and 3 points, respectively. Posterior pelvic injuries B1 and B2 both received a score of 2. B3 received a score of 3 points. C0, C1, C2, and C3 received scores of 2, 3, 5, and 6 points, respectively. The scores assigned to neurological modifiers N0, N1, N2, N3, and NX were 0, 1, 2, 4, and 3, respectively. Case-specific modifiers M1, M2, M3, and M4 received scores of 0, 0, 1, and 2 points, respectively.

Conclusions:
The results of this study validate the hierarchical nature of the AO Spine Sacral Classification System. The Sacral AOSIS sets the foundation for further studies to develop a universally accepted treatment algorithm for the treatment of complex sacral injuries.

Level of Evidence:
Level IV—Diagnostic.
Biomarkers for Bile Acid Malabsorption in Diarrhea-predominant Irritable Bowel Syndrome
Authors: Liu Tianxu , Ma Muyuan , Li Kelei , Tan Wei , Yu Han , Wang Ling 
Published: March 2023

Background and Aim:
A clear relationship of biological indexes between bile acid malabsorption (BAM) and diarrhea-predominant irritable bowel syndrome (IBS-D) has not been well analyzed. This meta-analysis aimed to establish a more convenient method to diagnose BAM in IBS-D patients by comparing the differences in biomarkers between IBS-D patients and healthy people.

Methods:
Multiple databases were searched for relevant case-control studies. Indicators used to diagnose BAM included 75Se-homocholic acid taurine (SeHCAT), 7α-hydroxy-4-cholesten-3-one(C4), fibroblast growth factor-19 and 48-hour fecal bile acid (48FBA). The rate of BAM (SeHCAT) was calculated by using a random-effect model. The levels of C4, FGF19, and 48FBA were compared, and the overall effect size was combined by a fixed effect model.

Results:
The search strategy identified 10 relevant studies comprising 1034 IBS-D patients and 232 healthy volunteers. The pooled rate of BAM in IBS-D patients was 32% (according to SeHCAT; 95% CI: 24%–40%). The level of C4 in IBS-D patients was significantly higher than that in the control group (2.86 ng/mL; 95% CI: 1.09, 4.63); The level of FGF19 was significantly lower than that in the control group (−33.97 pg/mL; 95% CI: −51.13, −16.82); The level of 48FBA was significantly higher than that in the control group (0.059; 95% CI: 0.41, 0.77).

Conclusions:
The results mainly concluded serum C4 and FGF19 levels in IBS-D patients. Most of the studies have different normal cutoff points of serum C4 and FGF19 levels; the performance of each test should be further estimated. By comparing the levels of these biomarkers, BAM in patients with IBS-D could be identified more accurately, which would lead to more effective treatment.