bluuebunny/crossref_metadata_2025_split huggingface dataset:
Neurofilament light chain is increased in the parahippocampal cortex and associates with pathological hallmarks in Parkinson’s disease dementia
Authors: Frigerio Irene , Laansma Max A. , Lin Chen-Pei , Hermans Emma J. M. , Bouwman Maud M. A. , Bol John G. J. M. , Galis-de Graaf Yvon , Hepp Dagmar H. , Rozemuller Annemieke J. M. , Barkhof Frederik , van de Berg Wilma D. J. , Jonkman Laura E. 
Published: January 2023

AbstractBackgroundIncreased neurofilament levels in biofluids are commonly used as a proxy for neurodegeneration in several neurodegenerative disorders. In this study, we aimed to investigate the distribution of neurofilaments in the cerebral cortex of Parkinson’s disease (PD), PD with dementia (PDD) and dementia with Lewy bodies (DLB) donors, and its association with pathology load and MRI measures of atrophy and diffusivity.MethodsUsing a within-subject post-mortem MRI-pathology approach, we included 9 PD, 12 PDD/DLB and 18 age-matched control donors. Cortical thickness and mean diffusivity (MD) metrics were extracted respectively from 3DT1 and DTI at 3T in-situ MRI. After autopsy, pathological hallmarks (pSer129-αSyn, p-tau and amyloid-β load) together with neurofilament light-chain (NfL) and phosphorylated-neurofilament medium- and heavy-chain (p-NfM/H) immunoreactivity were quantified in seven cortical regions, and studied in detail with confocal-laser scanning microscopy. The correlations between MRI and pathological measures were studied using linear mixed models.ResultsCompared to controls, p-NfM/H immunoreactivity was increased in all cortical regions in PD and PDD/DLB, whereas NfL immunoreactivity was increased in the parahippocampal and entorhinal cortex in PDD/DLB. NfL-positive neurons showed degenerative morphological features and axonal fragmentation. The increased p-NfM/H correlated with p-tau load, and NfL correlated with pSer129-αSyn but more strongly with p-tau load in PDD/DLB. Lastly, neurofilament immunoreactivity correlated with cortical thinning in PD and with increased cortical MD in PDD/DLB.ConclusionsTaken together, increased neurofilament immunoreactivity suggests underlying axonal injury and neurofilament accumulation in morphologically altered neurons with increased pathological burden. Importantly, we demonstrate that such neurofilament markers at least partly explain MRI measures that are associated with the neurodegenerative process.
Cerebellar granule neurons induce Cyclin D1 before the onset of motor symptoms in Huntington’s disease mice
Authors: Bauer Susanne , Chen Chwen-Yu , Jonson Maria , Kaczmarczyk Lech , Magadi Srivathsa Subramanya , Jackson Walker S. 
Published: January 2023

AbstractAlthough Huntington’s disease (HD) is classically defined by the selective vulnerability of striatal projection neurons, there is increasing evidence that cerebellar degeneration modulates clinical symptoms. However, little is known about cell type-specific responses of cerebellar neurons in HD. To dissect early disease mechanisms in the cerebellum and cerebrum, we analyzed translatomes of neuronal cell types from both regions in a new HD mouse model. For this, HdhQ200 knock-in mice were backcrossed with the calm 129S4 strain, to constrain experimental noise caused by variable hyperactivity of mice in a C57BL/6 background. Behavioral and neuropathological characterization showed that these S4-HdhQ200 mice had very mild behavioral abnormalities starting around 12 months of age that remained mild up to 18 months. By 9 months, we observed abundant Huntingtin-positive neuronal intranuclear inclusions (NIIs) in the striatum and cerebellum. The translatome analysis of GABAergic cells of the cerebrum further confirmed changes typical of HD-induced striatal pathology. Surprisingly, we observed the strongest response with 626 differentially expressed genes in glutamatergic neurons of the cerebellum, a population consisting primarily of granule cells, commonly considered disease resistant. Our findings suggest vesicular fusion and exocytosis, as well as differentiation-related pathways are affected in these neurons. Furthermore, increased expression of cyclin D1 (Ccnd1) in the granular layer and upregulated expression of polycomb group complex protein genes and cell cycle regulators Cbx2, Cbx4 and Cbx8 point to a putative role of aberrant cell cycle regulation in cerebellar granule cells in early disease.
Risk assessment of city site landscapes in the central plains of China
Authors: Li Wei , Yang Ruixia , Yan Lijie , Xie Yihan , Liu Honghao , Chen Fulong 
Published: January 2023

Abstract
Scientific cognition and assessment of threats to cultural heritage and surrounding landscapes are prerequisites for targeted management and protection of cultural heritage. This study took 530 city sites in the Central Plains of China as the research object. Based on the survey information of city sites in the third national cultural heritage survey in China, a combination of high resolution google images and land use/land cover data products and city sites survey data enables a comprehensive evaluation of landscape elements impacts on city sites. With spatial statistical method, we divided city site landscape types, and constructed a landscape element risk assessment model and an urbanization intensity index to analyze the threats of each landscape element to city site protection. The results indicated that 40.64% of the sites are in farmland landscapes, where agricultural cultivation, irrigation, and other production activities may affect the surface and subsurface remains of the sites. The sites (16.82%) in urban landscapes are affected by activities such as building houses and roads. Only six sites belong to the water landscape area, which may be affected by water erosion. Other sites (2.84%) are mainly affected by plant root growth in forest landscapes. The city sites distributed in urban areas such as Zhengzhou or the urban–rural junction were significantly affected by urban expansion and got the greatest protection pressure. City sites in agricultural landscapes have the second highest conservation risk. Plant erosion and flooding mainly affected the city sites in the northwest-southwest mountainous areas or near the water area of the study area, and the risks were relatively small. It can be seen that landscape types such as urban and farmland, which are closely related to human activities, bring the most pressure to the protection of city sites, and it is necessary to focus on the impact of human activities such as urban construction on city sites and the surrounding environment in the future, while paying attention to the protection and rational use of city sites with high assessment risk in urban planning and management for the protection of the city site proper and landscape scenery.
Who is on the right track? Behavior-based prediction of diagnostic success in a collaborative diagnostic reasoning simulation
Authors: Richters Constanze , Stadler Matthias , Radkowitsch Anika , Schmidmaier Ralf , Fischer Martin R. , Fischer Frank 
Published: January 2023

Abstract
Background
Making accurate diagnoses in teams requires complex collaborative diagnostic reasoning skills, which require extensive training. In this study, we investigated broad content-independent behavioral indicators of diagnostic accuracy and checked whether and how quickly diagnostic accuracy could be predicted from these behavioral indicators when they were displayed in a collaborative diagnostic reasoning simulation.

Methods
A total of 73 medical students and 25 physicians were asked to diagnose patient cases in a medical training simulation with the help of an agent-based radiologist. Log files were automatically coded for collaborative diagnostic activities (CDAs; i.e., evidence generation, sharing and eliciting of evidence and hypotheses, drawing conclusions). These codes were transformed into bigrams that contained information about the time spent on and transitions between CDAs. Support vector machines with linear kernels, random forests, and gradient boosting machines were trained to classify whether a diagnostician could provide the correct diagnosis on the basis of the CDAs.

Results
All algorithms performed well in predicting diagnostic accuracy in the training and testing phases. Yet, the random forest was selected as the final model because of its better performance (kappa = .40) in the testing phase. The model predicted diagnostic success with higher precision than it predicted diagnostic failure (sensitivity = .90; specificity = .46). A reliable prediction of diagnostic success was possible after about two thirds of the median time spent on the diagnostic task. Most important for the prediction of diagnostic accuracy was the time spent on certain individual activities, such as evidence generation (typical for accurate diagnoses), and collaborative activities, such as sharing and eliciting evidence (typical for inaccurate diagnoses).

Conclusions
This study advances the understanding of differences in the collaborative diagnostic reasoning processes of successful and unsuccessful diagnosticians. Taking time to generate evidence at the beginning of the diagnostic task can help build an initial adequate representation of the diagnostic case that prestructures subsequent collaborative activities and is crucial for making accurate diagnoses. This information could be used to provide adaptive process-based feedback on whether learners are on the right diagnostic track. Moreover, early instructional support in a diagnostic training task might help diagnosticians improve such individual diagnostic activities and prepare for effective collaboration. In addition, the ability to identify successful diagnosticians even before task completion might help adjust task difficulty to learners in real time.
Uncertainties in physical properties of Itokawa-like asteroids widen constraints on their formation time
Authors: Hallstrom Jonas , Bose Maitrayee 
Published: January 2023

AbstractOne of the outstanding questions in planetary science is to determine how the fundamental mechanical and physical properties of materials determine the thermal evolution of asteroids, and which properties have the greatest influence. We investigate the effects of uncertainty in the material properties of asteroid parent bodies on the ability of thermal evolution models to constrain the sizes and formation times of ordinary chondrite parent asteroids. A simple model is formulated for the thermal evolution of the parent body of asteroid 25143 Itokawa. The effects of the uncertainties in the values specified for specific heat capacity, thermal diffusivity, and aluminum abundance are determined. The uncertainties in specific heat capacity and aluminum abundance, or heat production more generally, are found to both have significant and approximately equal effects on these results, substantially widening the range of possible formation times of Itokawa’s parent body. We show that Itokawa’s parent body could have formed between 1.6 and 2.5 million years after the origin of calcium–aluminum inclusions with a radius larger than 19 km, and it could have formed as early as 1.4 millions years, as late as 3.5 million years, or with a radius as small at 17 km if more lenient definitions of uncertainty in aluminum abundance are considered. These results stress the importance of precise data required of the material properties of a suite of LL type 4-6 ordinary chondrite meteorites to place better constraints on the thermal history of Itokawa’s parent body.
Graphical Abstract
Electroencephalographic monitoring of brain activity during cardiac arrest: a narrative review
Authors: Roberti Elisabetta , Chiarini Giovanni , Latronico Nicola , Adami Enrica Chiara , Plotti Chiara , Bonetta Elisa , Magri Federica , Rasulo Frank Anthony ,   the Coma following Cardiac ArreST study group (COAST)
Published: January 2023

Abstract
Background
To date cardiac arrest (CA) remains a frequent cause of morbidity and mortality: despite advances in cardiopulmonary resuscitation (CPR), survival is still burdened by hypoxic–ischemic brain injury (HIBI), and poor neurological outcome, eventually leading to withdrawal of life sustaining treatment (WLST). The aim of CPR is cardiac pump support to preserve organ perfusion, until normal cardiac function is restored. However, clinical parameters of target organ end-perfusion during CPR, particularly brain perfusion, are still to be identified. In this context, electroencephalography (EEG) and its derivatives, such as processed EEG, could be used to assess brain function during CA.

Objectives
We aimed to review literature regarding the feasibility of EEG and processed or raw EEG monitoring during CPR.

Methods
A review of the available literature was performed and consisted of mostly case reports and observational studies in both humans and animals, for a total number of 22 relevant studies.

Results
The research strategy identified 22 unique articles. 4 observational studies were included and 6 animal testing studies in swine models. The remaining studies were case reports. Literature regarding this topic consists of conflicting results, containing studies where the feasibility of EEG during CPR was positive, and others where the authors reached opposite conclusions. Furthermore, the level of evidence, in general, remains low.

Discussion
EEG may represent a useful tool to assess CPR effectiveness. A multimodal approach including other non-invasive tools such as, quantitative infrared pupillometry and transcranial Doppler, could help to optimize the quality of resuscitation maneuvers.
Synergistic effects of dendrosomal nanocurcumin and oxaliplatin on oncogenic properties of ovarian cancer cell lines by down-expression of MMPs
Authors: Seyed Hosseini Elahe , Alizadeh Zarei Marziyeh , Tarrahimofrad Hossein , Zamani Javad , Haddad Kashani Hamed , Ahmad Ejaz , Nikzad Hossein 
Published: January 2023

Abstract
Background
Contrary to the advantageous anticancer activities of curcumin (Cur), limited bioavailability and solubility hindered its efficacy. Here, nontoxic dendrosomal nano carrier with Cur was used to overcome these problems. Despite considerable antitumor properties of Oxaliplatin (Oxa), the limiting factors are drug resistance and adverse side-effects. The hypothesis of this study was to evaluate the possible synergism between dendrosomal nanocurcumin (DNC) and Oxa and these agents showed growth regulatory effects on SKOV3 and OVCAR3 cells.

Methods and materials
In the present study, colony formation, wound healing motility, cell adhesion, transwell invasion and migration assay and cell cycle arrest with or without DNC, Oxa and Combination were defined. In addition to, real time PCR and Western blot were used to analyze AKT, PI3K, PKC, JNK, P38 and MMPs mRNAs and proteins expressions. Docking of MMP-2-Cur, MMP-2-DNC and MMP-2-Oxa was performed and the results of all three complexes were simulated by molecular dynamics.

Results
Our findings illustrated that DNC had the greatest effect on cell death as compared to the Cur alone. Moreover, the growth inhibitory effects (such as cell death correlated to apoptosis) were more intense if Oxa was added followed by DNC at 4 h interval. However, insignificant effects were observed upon simultaneous addition of these two agents in both cell lines. Besides, a combination of agents synergistically alters the relative expression of MMP-9.

Conclusions
The docking results showed that His70 and Asp100 may play a key role at the MMP-2 binding site. The matrigel invasion as well as cell viability of ovarian cancer cell lines SKOV3 and OVCAR3 by DNC alone or in combination with Oxa was inhibited significantly. The inhibitory effects of these agents were due to the differential expression levels of MMP 2 and MMP 9 regulated by multiple downstream signaling cascades. From the molecular dynamic simulation studies, it was confirmed that DNC established a strong interaction with MMP-2.
Teachers’ agency in the implementation of an early childhood education policy program in schools in Bogotá, Colombia
Authors: Guerrero Alba Lucy , Camargo-Abello Marina 
Published: January 2023

AbstractThe childhood national policy in Colombia recognizes the importance of quality in early childhood education (ECE). In this context, the government of the district of Bogota (2012–2016) proposed the program “Quality preschool in the Public Educational System” to expand the coverage of early education and provide comprehensive care to children aged 3–5 years within the public educational system. This article explores the ways in which the policy program is implemented in schools. Employing an ethnographic approach, this study focuses on the experiences of teachers from five schools that have faced the challenges of integrating the two new grades of early childhood education in formal school settings. By analyzing the educational strategies designed and implemented by teachers in their daily routines and the meaning they assign to these strategies, this study provides evidence that teachers do not simply reproduce the institutionalized discourses; instead, teachers negotiate, resist, adapt, and recreate experiences in relation to school times, spaces, activities, and materials by developing strategies more responsive to children’s needs. The concept of agency is central to understand how teachers end up generating practices that lead to new forms of individual and social transformation.
The influence of cannabis on sexual functioning and satisfaction
Authors: Moser Amanda , Ballard Sharon M. , Jensen Jake , Averett Paige 
Published: January 2023

Abstract
Background
The purpose of this study was to examine the perceived influence of cannabis on sexual functioning and satisfaction. This study used Kaplan’s and Masters and Johnson’s sexual response cycle (desire, excitement, orgasm, plateau, resolution) and included satisfaction to complete the sexual response cycle. Given increased attention in the research literature to the potential benefits of cannabis and the lack of research on the sexual benefits of cannabis use, the current study was completed.

Methods
Data were collected using the online survey tool “Qualtrics” from a self-selected, convenience sample of adults over the age of 18 who reported previous cannabis use. The survey, developed by the researchers based on previous literature, included demographic questions followed by a scale to measure sexual functioning and satisfaction in relation to cannabis use (α = 0.897).

Results
The final sample was 811 participants ranging in age from 18 to 85 years old (M = 32.11). The majority of participants were identified as female (n = 536, 64.9%), White/Caucasian (n = 640, 78.9%), and college educated (n = 650, 80.1%). Almost 25% of the participants were identified as LGBTQIA+ (n = 187, 23.1%). Most of the participants reported being in a monogamous sexual relationship (n = 598, 73.7%). Data were analyzed using descriptive statistics, t-tests, one-way ANOVA, and multiple regression. Age and gender were not found to have significant effects on cannabis use and sexual functioning and satisfaction. Over 70% of participants reported increased desire (M = 4.05, SD = 0.962) and orgasm intensity (M = 4.05, SD = 0.884). Participants who reported masturbating indicated that cannabis enhanced their pleasure while masturbating (n = 620, 62.5%). Participants also stated that cannabis enhanced their sense of taste (n = 583, 71.9%) and touch (n = 576, 71.0%).

Discussion
The results of this study contrast and establish new evidence within the literature. Demographic results indicate that the people who use cannabis are of a wide range of ages, from a variety of occupations, and have differing cannabis use preferences. The inclusion of LGBTQIA + respondents is a strength of this study. Overall, results indicated that both men and women perceived that cannabis use increased their sexual functioning and satisfaction, particularly increased desire and orgasm intensity.

Conclusion
This study updates the current literature on cannabis and sexuality and provides implications for improving sexual quality. Medical implications of this study include the possible use of cannabis for treating sexual dysfunctions, especially within women.
Baudelaire « fictionné »
Authors: Dupont Jacques 
Published: January 2023

Résumé
Le Crénom, Baudelaire ! de Jean Teulé se présente comme un « roman », comme une fiction prenant appui sur la vie de Baudelaire, telle que ses biographes l’ont racontée. À première vue, le narrateur veille à confondre sa voix avec celle de Baudelaire, voire à la subordonner à celle du poète, en usant d’une mosaïque de citations, et à user de documents divers ou de photographies afin de garantir l’ancrage de sa fiction dans une sorte de vérité biographique. Mais en définitive c’est l’imagination dépréciative du narrateur qui prévaut sur sa documentation, et qui s’emploie à placer Baudelaire dans de nombreuses situations peu flatteuses, ce qui débouche, de fait, sur un réquisitoire quasi systématique. La voix du narrateur prévaut aussi par une forme d’appropriation ou d’annexion négative : les citations de Baudelaire sont fréquemment recyclées hors de leur contexte, privées de leur qualité poétique à mesure qu’elles sont intégrées aux propos volontiers prosaïques ou vulgaires du narrateur, et ainsi dotées d’un statut ancillaire. Dans ce roman inutile, soumettre la vie de Baudelaire à une insistante et triviale incrimination signifie aussi dégrader les écrits du poète.
What I Learned about Teaching while TeachingMrs. Dallowayduring the Pandemic
Authors: Carillo Ellen C. 
Published: January 2023

AbstractThis article recounts the experience of moving an in-person literature class online at the start of the COVID-19 pandemic. Drawing comparisons between the novel Mrs. Dalloway, which the class was reading at the time, and the experience of the early days of the pandemic, the piece outlines how the exigencies of the pandemic led to revised teaching and assessment practices.
Off-Center Articulations
Authors: Chan Cheow Thia 
Published: January 2023

Abstract
Recent studies on Singapore Chinese literature have employed analytical lenses such as the Sinophone and postloyalism, which are exogenous to the historical and everyday experiences in the region that produced the texts. This article proposes using the lens of the Chinese-educated to bridge local self-understandings with extralocal modes of interpretation, in order to better illuminate place-specific writing practices. As a salient category of both lived experience and analysis by local researchers, the category of the Chinese-educated occasions a form of “off-center articulation” that maintains strategic distance from Sinophone studies while also enriching the field's conceptual repertoire. Specifically, this analytical perspective highlights how literary representations of social class play a significant role, alongside language and ethnicity, in registering the historical diversity of the Singapore Chinese community. Through examining Singaporean Chinese writer Chia Joo Ming's novel Exile or Pursuit (2015), this article reinterprets the novel's gallery of characters and depictions of interpersonal relations to elicit fading memories of socioeconomic divides and gaps in cultural attainment among ethnic Chinese Singaporeans and their migrant predecessors. It ends by charting future directions for Southeast Asian Chinese literary studies that collectively track a broader locus of “Chinese-educated” literary and cultural practices, and that promote critical inter-referencing within the region.