bluuebunny/crossref_metadata_2025_split huggingface dataset:
ОСОБЛИВОСТІ НАВЧАННЯ ТА ФОРМУВАННЯ СТУДЕНТІВ-СТОМАТОЛОГІВ В УМОВАХ ВІЙСЬКОВОГО СТАНУ
Authors: Гутор Н. С. 
Published: January 2023

Тернопільський національний медичний університет імені І. Я. Горбачевського МОЗ України постійно відповідає умовам та часу. Під час військового стану в Україні відбулося багато змін, які відрізняються від навчального процесу, що проводився раніше. Насамперед доводилося розробляти алгоритми, в яких йдеться про безпеку здобувачів та персоналу кафедри. Після облаштування безпечного укриття розпочався навчальний процес. Проте підготовка лікарів-стоматологів, які викувані сьогоденням, лише загартовує їх, зміцнює їх моральний дух та підштовхує до більш наполегливої праці. Здобувачі-стоматологи показали та продемонстрували свою свідомість українського громадянина Української держави. При підготовці майбутніх лікарів-стоматологів враховуємо надання допомоги в умовах війни.
Мета статті – узагальнити досвід підготовки лікарів-стоматологів на кафедрі хірургічної стоматології ТНМУ імені І. Я. Горбачевського, які навчаються за освітньо-професійною програмою «Стоматологія» другого (магістерського) рівня вищої освіти галузі знань 22 «Охорона здоров’я», спеціальності 221 «Стоматологія» в умовах військового стану в Україні.
ІНТЕРАКТИВНІ МЕТОДИ НАВЧАННЯ – ШЛЯХ ДО ПІДВИЩЕННЯ ЕФЕКТИВНОСТІ ОСВІТНЬОГО ПРОЦЕСУ У МЕДИЧНИХ ЗАКЛАДАХ ВИЩОЇ ОСВІТИ
Authors: Рожнова Т. Є. , Вадзюк С. Н. , Наконечна С. С. , Ратинська О. М. 
Published: January 2023

Вища освіта залишається важливою складовою сучасної спільноти, проте в умовах традиційних форм і методів навчання студенти, пасивно отримуючи інформацію, не вміють здобувати її самостійно й застосовувати те, що знають. Пріоритетом сучасної медичної освіти повинна стати особистість студента-медика, тому на сьогодні актуальності набувають інтерактивні методи навчання, що повинно привести до якісної зміни педагогічного процесу. Інтерактивні методи навчання є предметом численних досліджень, проте комплексного дослідження проблеми підвищення якості професійної підготовки в медичних ЗВО у процесі вивчення дисциплін із застосуванням інтерактивних методів навчання поки що немає.
Мета – обґрунтування застосування інтерактивних методів викладання у професійній підготовці студентів-медиків.
Впровадження інтерактивних методів навчання є перспективним напрямком навчання; їх впровадження сприяє створенню додаткових можливостей для оновлення змісту навчання, методів викладання дисциплін і розповсюдження знань, які ґрунтуються на сучасних мультимедійних технологіях. Інтерактивне навчання дає можливість урізноманітнити навчальний процес, що також є фактором підвищення інтересу до дисципліни та мотивації.
ВПЛИВ КРАНІОСКЕЛЕТНОЇ ТРАВМИ ТА ІНТЕНСИВНІСТЬ ПРОЦЕСІВ ЛІПІДНОЇ ПЕРОКСИДАЦІЇ В НИРЦІ ЗА УМОВ ХРОНІЧНОГО ГЕПАТИТУ І ЙОГО КОРЕКЦІЯ
Authors: Прохоренко О. О. 
Published: January 2023

Вступ. Поєднане ураження черепа та кісток скелета характеризується значною тяжкістю і високою летальністю. Недостатньо вивченим на цьому тлі є вплив супутньої патології, зумовленої хронічними дифузними захворюваннями печінки. У патогенезі обох патологічних процесів мають місце інтенсифікація ліпідної пероксидації та виснаження антиоксидантного захисту, що торкається практично всіх органів і тканин.
Мета дослідження – з’ясувати динаміку активності ензимної ланки антиоксидантного захисту в нир­ці за умов краніоскелетної травми та хронічного гепатиту й оцінити ефективність корекції армадіном.
Методи дослідження. В окремих групах білих щурів-самців моделювали краніоскелетну травму, хронічний гепатит та їх поєднання. З метою корекції у групі щурів з поєднаним ураженням застосовували армадін у дозі 20 мг/кг внутрішньочеревно. Через 14, 21, 28 і 35 діб після нанесення травми в нирці піддослідних тварин визначали супероксиддисмутазну і каталазну активність.
Результати й обговорення. Нанесення краніоскелетної травми на тлі хронічного гепатиту в усі терміни спостереження зумовлювало істотне зниження досліджуваних показників антиоксидантного захисту в нирці порівняно з контролем з досягненням мінімального рівня через 21 добу та підвищенням до 35-ї доби, яке не досягало рівня контролю. Величина досліджуваних показників у всі терміни посттравматичного періоду була істотно меншою, ніж у травмованих тварин без хронічного гепатиту. Застосування армадіну в щурів із краніоскелетною травмою та хронічним гепатитом, порівняно з аналогічною групою тварин без гепатиту, викликало істотне зростання досліджуваних показників антиоксидантного захисту в нирці, починаючи з 21-ї доби спостереження. За цих умов каталазна активність у нирці з 28-ї доби досягала рівня контрольної групи.
Висновки. Нанесення краніоскелетної травми на тлі хронічного гепатиту в період пізніх проявів травматичної хвороби (через 14, 21, 28 та 35 діб), порівняно з травмованими тваринами без хронічного ураження печінки, зумовлює більше зниження показників ензимної ланки антиоксидантного захисту в нирці (супероксиддисмутазної і каталазної активності). Застосування 3-оксипіридин 2-етил-6-метил-3-гідроксипіридину сукцинату в щурів із краніоскелетною травмою та хронічним гепатитом, порівняно з аналогічною групою тварин без корекції, починаючи з 21-ї доби після нанесення травми, викликає істотне зростання показників ензимної ланки антиоксидантного захисту.
Generative Logics and Conceptual Clicks: A Case Study of the Method for Design Materialization
Authors: Khaled Rilla , Barr Pippin 
Published: January 2023

Abstract
Game design research is caught between epistemologies and disciplines, leading to a lack of grounded methodologies. Without such methodological approaches, we cannot support evidence-based claims about game design. The Method for Design Materialization (MDM) is a methodological approach to capturing game design via software version control. By embracing a time-sliced representation of a game throughout its development and pairing it with explicit materializations of design thinking through reflective writing, we believe significant progress can be made in understanding and extrapolating from the design process.
Bioactive Decellularized Tendon-Derived Stem Cell Sheet for Promoting Graft Healing After Anterior Cruciate Ligament Reconstruction
Authors: Yao Shiyi , Liang Zuru , Lee Yuk Wa , Yung Patrick Shu Hang , Lui Pauline Po Yee 
Published: January 2023

Background: Stem cell sheets provide a scaffold-free option for the promotion of graft healing after anterior cruciate ligament reconstruction (ACLR). However, cell viability, stability, and potential uncontrolled actions create challenges for clinical translation. The decellularization of cell sheets may overcome these problems as studies have shown that the natural extracellular matrix of stem cells is bioactive and can promote tissue repair. Hypothesis: The decellularized tendon-derived stem cell (dTDSC) sheet can promote graft healing after ACLR. Study Design: Controlled laboratory study. Methods: An optimized decellularization protocol was developed to decellularize the TDSC sheets. A total of 64 Sprague-Dawley rats underwent ACLR with or without the dTDSC sheet wrapping the tendon graft (n = 32/group). At 2 and 6 weeks after surgery, graft healing was assessed by micro–computed tomography, histology, and biomechanical testing. The accumulation of iNOS+ and CD206+ cells and the expression of metalloproteinase 1 (MMP-1), MMP-13, and tissue inhibitor of metalloprotease 1 (TIMP-1) were assessed by immunohistochemistry. Results: The decellularization was successful, with the removal of 98.4% nucleic acid while preserving the collagenous proteins and bioactive factors. The expression of bone morphogenetic protein 2 (BMP-2) and VEGF in the dTDSC sheet was comparable with the TDSC sheet ( P > .05). Micro–computed tomography showed significantly more tunnel bone formation in the dTDSC sheet group. The dTDSC sheet group demonstrated better graft osteointegration and higher integrity of graft midsubstance with significantly higher ultimate failure load (16.58 ± 7.24 vs 8.93 ± 2.45 N; P = .002) and stiffness (11.97 ± 5.21 vs 6.73 ± 2.20 N/mm; P = .027). Significantly fewer iNOS+ cells but more CD206+ cells, as well as lower MMP-1 and MMP-13 but higher TIMP-1 expression, were detected at the tendon-bone interface and graft midsubstance in the dTDSC sheet group. Conclusion: An optimized decellularization protocol for producing bioactive dTDSC sheets was developed. Wrapping tendon graft with a dTDSC sheet promoted graft healing after ACLR, likely via enhancing bone formation and angiogenesis by BMP-2 and VEGF, modulating macrophage polarization and MMP/TIMP expression, and physically protecting the tendon graft. Clinical Relevance: dTDSC sheets alleviate the quality control and safety concerns of cell transplantation and can be used as a cell-free alternative for the promotion of graft healing in ACLR.
Characterization of Medication Discrepancies and Interventions Resulting From Pharmacy-Led Medication Reconciliation in the Critical Care Setting
Authors: Magrum BrookeAnne , Smetana Keaton S. , Thompson Molly , Elefritz Jessica L. , Phelps Megan , Trolli Elizabeth , Murphy Claire V. 
Published: January 2023

Background: Medication reconciliation has been shown to reduce medication-related errors in hospitalized patients, but the impact of pharmacy-led medication reconciliation in the intensive care unit (ICU) has not been extensively studied. Methods: This was a retrospective chart review of patients with a pharmacy-led medication reconciliation on admission to an ICU between January 1st and March 31st, 2018. Pharmacy-led medication reconciliations were completed by pharmacists, pharmacy residents, and pharmacy students. The objective of this study was to describe medication discrepancies identified by pharmacy-led medication reconciliation and to evaluate the interventions following. Results: A total of 288 patients were screened and 247 met inclusion criteria. There were 1148 medication discrepancies identified resulting in an average of 4.65 discrepancies per patient. Medication addition (54.25%) and medication deletion (45.75%) were most common. Within 24 hours of medication reconciliation, 214 interventions were made to active orders. No differences were observed between discrepancies identified and type of pharmacy staff completing the medication reconciliation. Conclusions: This study identified a high rate of medication discrepancies on admission to the ICU. Furthermore, it describes the types of pharmacist interventions following pharmacy-led medication reconciliation. This process may be impactful to incorporate as a standard practice in ICUs and warrants further investigation into value, cost, and pharmacist workflow.
Comparing the effectiveness of L-carnitine and paraffin oil in acute aluminum phosphide poisoning using predictive biomarkers and scores: A randomized controlled clinical trial
Authors: Abdelhamid Walaa G , Sakr Mahmoud L , Mostafa Olfat E , Zaafar Dalia , Abdelwahab Hanan M 
Published: January 2023

Aluminum phosphide (AlP) poisoning is a serious medical emergency with a high mortality rate. The absence of an exact antidote for AlP poisoning necessitates the quest for alternative treatment options. The study sought to assess the efficacy of adding L-carnitine or medicated paraffin oil to the conventional approach of treatment employed in cases of acute AlP poisoning. We conducted a 1 year, randomized, controlled, parallel-group, single-blind clinical study. 96 individuals with acute AlP poisoning were randomly assigned to one of three groups. The standard AlP therapy was administered to all groups according to the Poison Control Center guidelines at the Ain-Shams University hospitals. All patients underwent a medical history review, clinical examination, and laboratory tests. The outcomes were assessed. The participants in the study groups had mean ages ranging from 25.6 to 26.3 years. The cases analyzed were evenly distributed between genders, with the majority originating from rural areas. The average delay time varied from 2.9 to 4.2 h. All patients in the study reported ingesting AlP during suicide attempts. 12 hours after admission, many clinical and biochemical data improved in both intervention groups including cytochrome c oxidase, caspase-3, caspase-9, catalase, and superoxide dismutase. The intervention groups required significantly less mechanical ventilation and had a lower mortality rate than the control group. Decontamination with paraffin oil could be advantageous for reducing the severity of AlP poisoning, improving prognosis, and lowering the mortality rate.
Longitudinal relations between autistic-like features and functional somatic symptoms in adolescence
Authors: Hogendoorn Elske , Hartman Catharina A , Burke Sarah M , van Dijk Marijn W G , Rosmalen Judith G M 
Published: January 2023

Autistic-like features and functional somatic symptoms (FSS) frequently co-occur. It remains unknown how autistic-like features and FSS affect each other and develop throughout adolescence. This study examined reciprocal relations between autistic-like features and FSS in adolescence. Participants were 2772 adolescents (52.5% male) from the Tracking Adolescents’ Individual Lives Survey population and clinical cohort. Data from four waves were included, covering the ages between 11 and 19 years. Autistic-like features were measured using the Children’s Social Behavior Questionnaire. FSS were assessed using the Youth Self Report and Adult Self Report, respectively. Using the random intercept cross-lagged panel model, a stable positive, moderately strong between-persons association was found between autistic-like features and FSS. No within-persons reciprocal effects from wave to wave were observed. Secondary analyses revealed a consistent relation with FSS for three different domains of autistic-like features (social and communication behaviors, repetitive behaviors, and self-regulatory behaviors), and highly similar interrelations in a subsample of adolescents with a clinical autism spectrum disorder diagnosis. In conclusion, the co-occurrence between autistic-like features and FSS is stable throughout adolescence. Clinicians working with adolescents with autistic-like features should be alert to the presence FSS, and vice versa. Lay abstract Adolescents with autistic-like features frequently experience unexplained somatic symptoms too, and vice versa. We followed 2772 adolescents for 8 years, starting at 11 and ending at 19 years of age. At four different moments during this time, we asked these adolescents how often they suffered from unexplained somatic symptoms, such as stomachache and dizziness. We asked their parents to what extent the adolescents showed autistic-like features at those four moments too. Then, we assessed whether the relation between autistic-like features and unexplained somatic symptoms stayed the same between 11 and 19 years old. We also looked at whether there was a reciprocal influence. So far, most studies only looked into the relation between autistic-like features and unexplained somatic symptoms at a specific moment in time. It is important to study how this relation develops over time in adolescence, so we can improve treatment for burdening co-occurring symptoms. In our sample, adolescents who experienced many autistic symptoms also experienced many unexplained somatic symptoms. This relation stayed the same over time. There was no reciprocal influence, so higher autistic-like features did not contribute to higher unexplained somatic symptoms, or the other way around. The findings of this work tell us that in adolescents with autistic-like features it is important to be alert to the presence of unexplained somatic symptoms, and vice versa.
New Noncoding Base Pair Mutation at the Identical Locus as the Original NCMD/MCDR1 in a Mexican Family, Suggesting a Mutational Hotspot
Authors: Small Kent W. , Van de Sompele Stijn , Avetisjan Jessica , Udar Nitin , Agemy Steven , De Baere Elfride , Shaya Fadi S. 
Published: January 2023

Purpose: To clinically and molecularly study a newly found family with North Carolina macular dystrophy (NCMD/MCDR1) from Mexico. Methods: This retrospective study comprised 6 members of a 3-generation Mexican family with NCMD. Clinical ophthalmic examinations, including fundus imaging, spectral-domain optical coherence tomography, electroretinography, and electrooculography, were performed. Genotyping with polymorphic markers in the MCDR1 region was performed to determine haplotypes. Whole-genome sequencing (WGS) was performed followed by variant filtering and copy number variant analysis. Results: Four subjects from 3 generations were found to have macular abnormalities. The proband presented with lifelong bilateral vision impairment with bilaterally symmetric vitelliform Best disease-like appearing macular lesions. Her 2 children had bilateral large macular coloboma-like malformations, consistent with autosomal dominant NCMD. The 80-year-old mother of the proband had drusen-like lesions consistent with grade 1 NCMD. WGS and subsequent Sanger sequencing found a point mutation at chr6:99593030G>C (hg38) in the noncoding region of the DNase I site thought to be a regulatory element of the retinal transcription factor gene PRDM13. This mutation is the identical site/nucleotide as in the original NCMD family (#765) but is a guanine to cytosine change rather than a guanine to thymine mutation, as found in the original NCMD family. Conclusions: We report a new noncoding mutation at the same locus (chr6:99593030G>C) involving the same DNase I site regulating the retinal transcription factor gene PRDM13. This suggests that this site, chr6:99593030, is a mutational hotspot.
Determinants of substrate specificity in a catalytically diverse family of acyl-ACP thioesterases from plants
Authors: Kalinger Rebecca S. , Rowland Owen 
Published: January 2023

Abstract
Background
ACYL-LIPID THIOESTERASES (ALTs) are a subclass of plastid-localized, fatty acyl-acyl carrier protein (ACP) thioesterase enzymes from plants. They belong to the single hot dog-fold protein family. ALT enzymes generate medium-chain (C6-C14) and C16 fatty acids, methylketone precursors (β-keto fatty acids), and 3-hydroxy fatty acids when expressed heterologously in E. coli. The diverse substrate chain-length and oxidation state preferences of ALTs set them apart from other plant acyl-ACP thioesterases, and ALTs show promise as metabolic engineering tools to produce high-value medium-chain fatty acids and methylketones in bacterial or plant systems. Here, we used a targeted motif-swapping approach to explore connections between ALT protein sequence and substrate specificity. Guided by comparative motif searches and computational modelling, we exchanged regions of amino acid sequence between ALT-type thioesterases from Arabidopsis thaliana, Medicago truncatula, and Zea mays to create chimeric ALT proteins.

Results
Comparing the activity profiles of chimeric ALTs in E. coli to their wild-type counterparts led to the identification of interacting regions within the thioesterase domain that shape substrate specificity and enzyme activity. Notably, the presence of a 31-CQH[G/C]RH-36 motif on the central α-helix was shown to shift chain-length specificity towards 12–14 carbon chains, and to be a core determinant of substrate specificity in ALT-type thioesterases with preference for 12–14 carbon 3-hydroxyacyl- and β-ketoacyl-ACP substrates. For an ALT containing this motif to be functional, an additional 108-KXXA-111 motif and compatible sequence spanning aa77–93 of the surrounding β-sheet must also be present, demonstrating that interactions between residues in these regions of the catalytic domain are critical to thioesterase activity. The behaviour of chimeric enzymes in E. coli also indicated that aa77–93 play a significant role in dictating whether an ALT will prefer ≤10-carbon or ≥ 12-carbon acyl chain-lengths, and aa91–96 influence selectivity for substrates of fully or partially reduced oxidation states. Additionally, aa64–67 on the hot dog-fold β-sheet were shown to be important for enabling an ALT to act on 3-hydroxy fatty acyl-ACP substrates.

Conclusions
By revealing connections between thioesterase sequence and substrate specificity, this study is an advancement towards engineering recombinant ALTs with product profiles suited for specific applications.
Effect of topical fluoride applications on residual monomer release from resin-based restorative materials
Authors: Delikan Ebru , Erturk-Avunduk Ayse Tugba , Karatas Ozcan , Saçmacı Şerife 
Published: January 2023

Abstract
Background
The effects of topical fluoride applications on the release of monomer ingredients from resin-based dental materials by immersion in various extraction solutions are unclear. The aim of this study was to determine the effect of topical fluorides (APF and NaF) on the elution of residual monomers (Bis-GMA, TEGDMA, UDMA, and HEMA) from resin-based materials.

Methods
Ninety specimens were prepared, 30 bulk-fill composite resin, 30 nanohybrid universal composite resin, and 30 polyacid-modified composite resin (compomer). These were randomly divided into three groups based on fluoride application procedures. Each specimen was kept in 75% ethanol solution, and residual monomers released from materials were analyzed using high-performance liquid chromatography (HPLC) after 10 min, 1 h, 24 h, and 30 days. The groups were compared using the Mann Whitney U and Kruskal Wallis tests. Measurements were analyzed using the Friedman and Wilcoxon signed-rank tests.

Results
Fluoride applications generally had no considerable effect on the amount of residual monomer released from resin-based restorative materials. The amount of monomer release after topical APF application was similar to the release in the control group and was lower than the release in the NaF group. The release of monomers from the resin-based material used in the study did not approach toxic levels at the applied time intervals. The compomer released lower amounts of monomer than other resin-based materials.

Conclusions
Fluoride applications do not increase monomer release from resin-based restorative materials. However, compomers should be employed by clinicians due to their lower monomer release compared to other resin restorative materials. The release of monomers from all the resin-based materials did not approach toxic levels at the applied time intervals.
Purification and biochemical characterization of two laccase isoenzymes isolated from Trichoderma harzianum S7113 and its application for bisphenol A degradation
Authors: Elsayed Alshaimaa M. , Mahmoud Mohamed , Abdel Karim Ghada S. A. , Abdelraof Mohamed , Othman Abdelmageed M. 
Published: January 2023

AbstractTwo laccase isoenzymes (LacA and LacB) were isolated from a novelTrichoderma harzianumS7113 isolate employing ammonium sulfate precipitation, Sephadex G100, and DEAE Sepharose ion exchange chromatography. The molecular weights of the purified LacA and LacB laccases were estimated to be 63 and 48 kDa, respectively. The two isoenzymes had their optimum activities at the same temperature (50 °C), but at slightly different pH values (pH 3.0 for LacA and pH 2.5 for LacB). LacA and LacB had the same thermal stability at 40 °C and pH stability at pH 9.0. The two isoenzymes also showed a high level of specific activity toward ABTS, where theKmvalues of LacA and LacB were 0.100 and 0.065 mM, whereas theirVmaxvalues were 0.603 and 0.182 µmol min−1, respectively. LacA and LacB catalytic activity was stimulated by Mg2+, Zn2+, K+, and Ni2+, whereas it was inhibited by Hg2+and Pb2+, β-mercaptoethanol, EDTA, and SDS, and completely inhibited by sodium azide. Our findings indicate that purified laccase has a promising capacity for bisphenol A (BPA) bioremediation across a broad pH range. This finding opens up new opportunities for the commercialization of this technique in a variety of biotechnology-based applications, particularly for removing endocrine chemicals from the environment.