bluuebunny/crossref_metadata_2025_split huggingface dataset:
Acceptability, usability and feasibility of experienced sampling method in chronic secondary pain syndromes
Authors: Damci Aysun , Hoeijmakers Janneke G. J. , den Hollander Marlies , Köke Albère , de Mooij Marion , Faber Catharina G. , Verbunt Jeanine A. M. C. F. 
Published: July 2023

BackgroundIn chronic pain syndromes, symptoms can fluctuate and change over time. Standard questionnaires cannot register these fluctuations. Nonetheless, the experience sampling method (ESM) is developed to collect momentary measurements of everyday complaints, tracing fluctuations in symptoms and disabling factors over time. Although valuable information can be collected in this way, assessment may also be a burden. This study aimed to investigate the acceptability, usability, and feasibility of ESM in chronic secondary pain syndromes, in a single-center study in the Netherlands.MethodsA prospective observational study with repeated measurements was conducted in patients with chronic secondary neuropathic and musculoskeletal pain syndromes, including small fiber neuropathy, spinal cord injury, and rheumatoid disorder.ResultsThirty-four participants were included and filled in the ESM, of whom 19 were diagnosed with small fiber neuropathy, 11 with spinal cord injury, and 4 with a rheumatoid disorder. The mean age was 54.7 ± 13.9 years (range: 23–77) of whom 52.9% were female. In total, 19 participants filled in the general and user-friendliness evaluation about the acceptability and usability of the ESM. The general evaluation showed no influence of ESM on participants’ social contacts (mean 1.47, SD 1.12), activities (mean 1.74, SD 1.44), and mood (mean 1.89, SD 1.59). The answers options of ESM were a good representation of the experiences of participants (mean 4.58, SD 1.77). Regarding feasibility, the overall response rate for answering the beep signals of ESM was 44.5% in total. The missing rate per person varied from 13% to 97% with a median of 54.1%.ConclusionThe general evaluation and the user-friendliness revealed sufficient outcomes in favor of the ESM application. ESM seems a promising measurement tool to use in secondary chronic pain syndromes.
A defined method for differentiating human iPSCs into midbrain dopaminergic progenitors that safely restore motor deficits in Parkinson’s disease
Authors: Nakamura Ryota , Nonaka Risa , Oyama Genko , Jo Takayuki , Kamo Hikaru , Nuermaimaiti Maierdanjiang , Akamatsu Wado , Ishikawa Kei-ichi , Hattori Nobutaka 
Published: July 2023

BackgroundParkinson’s disease (PD) is a progressive neurodegenerative condition that primarily affects motor functions; it is caused by the loss of midbrain dopaminergic (mDA) neurons. The therapeutic effects of transplanting human-induced pluripotent stem cell (iPSC)-derived mDA neural progenitor cells in animal PD models are known and are being evaluated in an ongoing clinical trial. However, However, improvements in the safety and efficiency of differentiation-inducing methods are crucial for providing a larger scale of cell therapy studies. This study aimed to investigate the usefulness of dopaminergic progenitor cells derived from human iPSCs by our previously reported method, which promotes differentiation and neuronal maturation by treating iPSCs with three inhibitors at the start of induction.MethodsHealthy subject-derived iPS cells were induced into mDA progenitor cells by the CTraS-mediated method we previously reported, and their proprieties and dopaminergic differentiation efficiency were examined in vitro. Then, the induced mDA progenitors were transplanted into 6-hydroxydopamine-lesioned PD model mice, and their efficacy in improving motor function, cell viability, and differentiation ability in vivo was evaluated for 16 weeks.ResultsApproximately ≥80% of cells induced by this method without sorting expressed mDA progenitor markers and differentiated primarily into A9 dopaminergic neurons in vitro. After transplantation in 6-hydroxydopamine-lesioned PD model mice, more than 90% of the engrafted cells differentiated into the lineage of mDA neurons, and approximately 15% developed into mature mDA neurons without tumour formation. The grafted PD model mice also demonstrated significantly improved motor functions.ConclusionThis study suggests that the differentiation protocol for the preparation of mDA progenitors is a promising option for cell therapy in patients with PD.
High-altitude cerebral hypoxia promotes mitochondrial dysfunction and apoptosis of mouse neurons
Authors: Huan Yu , Quan Huilin , Jia Bo , Hao Guangzhi , Shi Zuolin , Zhao Tianzi , Yuan Ying , Yuan Fang , Dong Yushu , Liang Guobiao 
Published: July 2023

IntroductionNeuronal cell death is an important factor in the pathogenesis of acute high-altitude cerebral hypoxia; however, the underlying molecular mechanism remains unclear. In this study, we tested if high-altitude hypoxia (HAH) causes neuronal death and mitochondrial dysfunction using various in vivo and in vitro approaches.MethodsAcute high-altitude cerebral hypoxia was induced by hypobaric hypoxia chamber in male mice. we explored the mechanisms of neuronal cell death using immunofluorescence, western blotting, transmission electron microscopy, and flow cytometry. Next, mitochondrial function and morphology were observed using Jc-1 staining, seahorse assay, western blotting, MitoTracker staining, and transmission electron microscopy. Moreover, open field test, elevated plus test, and Morris water maze were applied for animal behavior.ResultsResults revealed that HAH disrupted mitochondrial function and promoted neuronal apoptosis and necroptosis both in HT-22 cells and in mouse hippocampal neurons. Moreover, the mitochondrial membrane potential and adenosine triphosphate production decreased in neurons after HAH, while oxidative stress and mitochondrial fission increased. Behavioral studies suggested that HAH induced anxiety-like behavior and impaired spatial memory, while it had no effect on athletic ability.DiscussionThese findings demonstrated that HAH promotes mitochondrial dysfunction and apoptosis of mouse neurons, thus providing new insights into the role of mitochondrial function and neuronal cell death in acute high-altitude cerebral hypoxia.
Temporal genomic heterogeneity guiding individualized therapy in recurrent non-small cell lung cancer
Authors: Fang Qiyu , Wan Xiaoying , D’Aiello Angelica , Sun Hui , Gu Weiquing , Li Yixue , Zhou Caicun , Xie Boxiong , Deng Qinfang , Cheng Haiying , Zhou Songwen 
Published: July 2023

IntroductionDespite the benefit of adjuvant systemic therapy for patients with resected non-small cell lung cancer (NSCLC), the risk of postoperative recurrence remains high. Our objective was to characterize temporal genetic heterogeneity between primary resected and recurrent tumors, and its impact on treatment outcomes.MethodsIn this study, next-generation sequencing (NGS) testing was performed on tissue specimens and circulating tumor DNA (ctDNA) collected at postoperative recurrence, and results were compared to the genotypes of initial surgical specimens.ResultsOf forty-five patients with matched primary and post-operative recurrent tumors, EGFR status switched in 17 patients (37.8%) at post-operative recurrence and 28 patients (62.2%) had no genotype change (17 mutant, 11 wild-type). Based on the changes of EGFR status, patients were divided into 4 groups. Following subsequent treatment with EGFR TKI o chemotherapy: In group A, with sustained sensitive mutation, the percentage achieving partial response (PR) was the highest, at 72.2%, the median progression-free survival (PFS) was 17 months, and the median overall survival (OS) was 44.0 months respectively; In group B, with genotype changed from wild-type to mutant, 50% achieved PR, PFS was 10 months, and OS was 35 months; In group C, in which mutant status shifted to wild-type or new co-mutation emerged, the percentage achieving PR was 30%, PFS was 9 months, and OS was 35 months. In group D, with sustained wild type, the percentage achieving PR was 27.3%, PFS was 8 months, and OS was 22 months.DiscussionGenotypic shift between paired primary and post-operative recurrent tumors was not infrequent, and this temporal genomic heterogeneity substantially impacted subsequent treatment outcomes.
Predicting cancer relapse following lung stereotactic radiotherapy: an external validation study using real-world evidence
Authors: Davey Angela , Thor Maria , van Herk Marcel , Faivre-Finn Corinne , Rimner Andreas , Deasy Joseph O. , McWilliam Alan 
Published: July 2023

PurposeFor patients receiving lung stereotactic ablative radiotherapy (SABR), evidence suggests that high peritumor density predicts an increased risk of microscopic disease (MDE) and local-regional failure, but only if there is low or heterogenous incidental dose surrounding the tumor (GTV). A data-mining method (Cox-per-radius) has been developed to investigate this dose-density interaction. We apply the method to predict local relapse (LR) and regional failure (RF) in patients with non-small cell lung cancer.Methods199 patients treated in a routine setting were collated from a single institution for training, and 76 patients from an external institution for validation. Three density metrics (mean, 90th percentile, standard deviation (SD)) were studied in 1mm annuli between 0.5cm inside and 2cm outside the GTV boundary. Dose SD and fraction of volume receiving less than 30Gy were studied in annuli 0.5-2cm outside the GTV to describe incidental MDE dosage. Heat-maps were created that correlate with changes in LR and RF rates due to the interaction between dose heterogeneity and density at each distance combination. Regions of significant improvement were studied in Cox proportional hazards models, and explored with and without re-fitting in external data. Correlations between the dose component of the interaction and common dose metrics were reported.ResultsLocal relapse occurred at a rate of 6.5% in the training cohort, and 18% in the validation cohort, which included larger and more centrally located tumors. High peritumor density in combination with high dose variability (0.5 - 1.6cm) predicts LR. No interactions predicted RF. The LR interaction improved the predictive ability compared to using clinical variables alone (optimism-adjusted C-index; 0.82 vs 0.76). Re-fitting model coefficients in external data confirmed the importance of this interaction (C-index; 0.86 vs 0.76). Dose variability in the 0.5-1.6 cm annular region strongly correlates with heterogeneity inside the target volume (SD; ρ = 0.53 training, ρ = 0.65 validation).ConclusionIn these real-world cohorts, the combination of relatively high peritumor density and high dose variability predicts increase in LR, but not RF, following lung SABR. This external validation justifies potential use of the model to increase low-dose CTV margins for high-risk patients.
Computational study of SENP1 in cancer by novel natural compounds and ZINC database screening
Authors: Taghvaei Somayye , Taghvaei Alireza , Anvar Mohammad Saberi , Guo Chun , Sabouni Farzaneh , Minuchehr Zarrin 
Published: July 2023

Introduction: Sentrin-specific protease 1 (SENP1) is a protein whose main function is deSUMOylation. SENP1 inhibits apoptosis, and increases angiogenesis, estrogen and androgen receptor transcription and c-Jun transcription factor, proliferation, growth, cell migration, and invasion of cancer. The in vivo and in vitro studies also demonstrated which natural compounds, especially phytochemicals, minerals, and vitamins, prevent cancer. More than 3,000 plant species have been reported in modern medicine. Natural compounds have many anti-cancerous andanti-turmeric properties such as antioxidative, antiangiogenic, antiproliferative, and pro-apoptotic properties.Methods: In this study, we investigated the interaction of some natural compounds with SENP1 to inhibit its activity. We also screened the ZINC database including natural compounds. Molecular docking was performed, and toxicity of compounds was determined; then, molecular dynamics simulation (MDS) and essential dynamics (ED) were performed on natural compounds with higher free binding energies and minimal side effects. By searching in a large library, virtual screening of the ZINC database was performed using LibDock and CDOCKER, and the final top 20 compounds were allowed for docking against SENP1. According to the docking study, the top three leading molecules were selected and further analyzed by MDS and ED.Results: The results suggest that resveratrol (from the selected compounds) and ZINC33916875 (from the ZINC database) could be more promising SENP1 inhibitory ligands.Discussion: Because these compounds can inhibit SENP1 activity, then they can be novel candidates for cancer treatment. However, wet laboratory experiments are needed to validate their efficacy as SENP1 inhibitors.
Screening of wild species and transcriptome profiling to identify differentially regulated genes in response to late blight resistance in potato
Authors: Bhatia Nisha , Tiwari Jagesh Kumar , Kumari Chandresh , Zinta Rasna , Sharma Sanjeev , Thakur Ajay Kumar , Buckseth Tanuja , Dalamu Dalamu , Singh Rajesh Kumar , Kumar Vinod 
Published: July 2023

Late blight (Phytophthora infestans) is a serious disease of potatoes. The aim of this study was to screen wild potato species and identify differentially expressed genes (DEGs) associated with late blight resistance. Wild potato species such as PIN45 (Solanum pinnatisectum), CPH62 (Solanum cardiophyllum), JAM07 (Solanum jamesii), MCD24 (Solanum microdontum), PLD47 (Solanum polyadenium), and cv. Kufri Bahar (control) were tested by artificial inoculation of P. infestans under controlled conditions. Transcriptomes of the leaf tissues (96 h post-inoculation) were sequenced using the Illumina platform. Statistically significant (p &lt; 0.05) DEGs were analyzed in wild species by comparison with the control, and upregulated (&gt;2 log2 fold change, FC) and downregulated (&lt;−2 log2 FC) genes were identified. DEGs were functionally characterized with Gene Ontology (GO) terms and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways. Selected genes were validated by real-time PCR analysis to confirm RNA-seq results. We identified some upregulated genes associated with late blight resistance in wild species such as cytochrome P450, proline-rich protein, MYB transcription factor MYB139, ankyrin repeat-containing protein, and LRR receptor-like serine/threonine-protein kinase in PIN45; glucosyltransferase, fructose-bisphosphate aldolase, and phytophthora-inhibited protease 1 in CPH62; steroid binding protein and cysteine proteinase 3 in JAM07; glycine-rich cell wall structural protein 1 and RING finger protein in MCD24; and cysteine proteinase 3 and major latex protein in PLD47. On the other hand, downregulated genes in these species were snakin-2 and WRKY transcription factor 3 in PIN45; lichenase and phenylalanine ammonia-lyase 1 in CPH62; metallothionein and LRR receptor-like serine/threonine-protein kinase in JAM07; UDP-glucoronosyl/UDP-glucosyl transferase family protein and steroid binding protein in MCD24; and cytoplasmic small heat shock protein class I and phosphatase PLD47. Our study identified highly resistant wild potato species and underlying genes such as disease resistance, stress response, phytohormones, and transcription factors (e.g., MYB, WRKY, AP2/ERF, and AN1) associated with late blight resistance in wild potato species.
Reduced suicidality after electroconvulsive therapy is linked to increased frontal brain activity in depressed patients: a resting-state fMRI study
Authors: Wang Xue , Wu Han , Wang Dan , Wang Wei , Wang Wen , Jin Wen-qing , Luo Jiong , Jiang Wei , Tang Yi-lang , Ren Yan-ping , Yang Chun-lin , Ma Xin , Li Rena 
Published: July 2023

ObjectiveSuicidality is commonly observed in patients with depressive episodes, and electroconvulsive therapy (ECT) has been found to be effective in treating these patients. However, the role of ECT in suicidality remains unclear. This study used resting-state functional magnetic resonance imaging (rs-fMRI) to explore the changes in brain function before and after ECT in depressed patients with suicidality.MethodsIn total, 26 depressed patients with suicidality underwent rs-fMRI at baseline and after 8–12 sessions of ECT. In addition, 32 healthy controls (HCs) matched for age, gender, and educational level underwent rs-fMRI once. The amplitude of low-frequency fluctuations (ALFF), the fractional amplitude of low-frequency fluctuations (fALFF), and regional homogeneity (ReHo) were measured to evaluate whole brain function. Differences between the groups and time points (before and after ECT) were compared. Clinical symptoms were assessed using the 17-item Hamilton Depression Scale (HAMD-17) and Beck Scale for Suicide Ideation (BSSI).ResultsAt baseline, patients exhibited decreased ALFF in the right postcentral and precentral gyrus and decreased fALFF in the right supramarginal and postcentral gyrus, left superior frontal gyrus (SFG), as well as the superior and middle temporal gyrus compared to HCs. Patients also had lower ReHo in the left amygdala, anterior cingulate, and postcentral gyrus, and in the right thalamus, insula, and postcentral gyrus. They also exhibited higher ALFF in the bilateral temporal gyrus and insula as well as higher fALFF in the cerebellum. Following ECT, fALFF in the left SFG and orbital frontal cortex (OFC) significantly increased and was inversely correlated with the reduction of BSSI scores (r = −0.416, p = 0.048), whereas no correlation was found with changes in HAMD-17scores.ConclusionOur findings suggest that the left SFG and OFC may play a key role in the mechanism of ECT for suicidality. The decrease of fALFF in the left SFG and OFC may represent a potential mechanism through which ECT effectively treats suicidality in depressed patients.
Revealing a Third Dissolved-Phase Xenon-129 Resonance in Blood Caused by Hemoglobin Glycation
Authors: Mikowska Lutosława , Grynko Vira , Shepelytskyi Yurii , Ruset Iullian C. , Deschamps Joseph , Aalto Hannah , Targosz-Korecka Marta , Balamore Dilip , Harańczyk Hubert , Albert Mitchell S. 
Published: July 2023

Hyperpolarized (HP) xenon-129 (129Xe), when dissolved in blood, has two NMR resonances: one in red blood cells (RBC) and one in plasma. The impact of numerous blood components on these resonances, however, has not yet been investigated. This study evaluates the effects of elevated glucose levels on the chemical shift (CS) and T2* relaxation times of HP 129Xe dissolved in sterile citrated sheep blood for the first time. HP 129Xe was mixed with sheep blood samples premixed with a stock glucose solution using a liquid–gas exchange module. Magnetic resonance spectroscopy was performed on a 3T clinical MRI scanner using a custom-built quadrature dual-tuned 129Xe/1H coil. We observed an additional resonance for the RBCs (129Xe-RBC1) for the increased glucose levels. The CS of 129Xe-RBC1 and 129Xe-plasma peaks did not change with glucose levels, while the CS of 129Xe-RBC2 (original RBC resonance) increased linearly at a rate of 0.015 ± 0.002 ppm/mM with glucose level. 129Xe-RBC1 T2* values increased nonlinearly from 1.58 ± 0.24 ms to 2.67 ± 0.40 ms. As a result of the increased glucose levels in blood samples, the novel additional HP 129Xe dissolved phase resonance was observed in blood and attributed to the 129Xe bound to glycated hemoglobin (HbA1c).
Las buenas prácticas en la educación inclusiva
Authors: Coahila Huallpa Evelin Susan 
Published: July 2023

Las buenas prácticas en la educación responden a las crisis y buscan soluciones alternativas para comunicarse y relacionarse con los estudiantes mediante los recursos de aprendizaje. De esta manera, el objetivo del estudio fue analizar las buenas prácticas en la educación inclusiva, para ello, se llevó a cabo una revisión sistemática considerando 50 artículos de los buscadores Researchgate, Scielo, Scopus, Ebsco y SciencieDirect, con operadores lógicos and, or, not, mediante las palabras claves “buenas prácticas en la educación inclusiva” “buenas prácticas” “buenas prácticas inclusivas” “buenas prácticas pedagógicas” “good practices in inclusive education” “good practices” “good inclusive practices” “good pedagogical practices”. Se concluye que cuando se habla de buenas prácticas, también conlleva al tema de inclusión, ya que debe estar dirigidos a todos los sectores estudiantiles, fomentando la participación al desarrollo del fortalecimiento del aprendizaje sin exclusi ón.
Logistics of Smart Port in Ukraine: Problems and Prospects in the Conditions of Sustainable Development
Authors: Muradian Arsen , Kuzkin Olexiy , Remzina Natalia 
Published: July 2023

Every day, the global logistics industry faces new challenges – both due to the Russian invasion of Ukraine and due to economic instability in global markets. The pandemic and the disruption of traditional supply chains are forcing companies to change their production geography. Accelerated digitization of people's and companies' livelihoods is contributing to the transformation of classical logistics, and in the end, those players who create an environment of additional services for delivery support, thereby forming resilience to risks, are winning. In this regard, there is a need to develop theoretical and applied aspects of the transition of port logistics to sustainable development through the introduction of smart port technologies that contribute to the achievement of a number of sustainable development goals, such as: 1: no poverty, 2: zero hunger, 3: good health and well-being, 8: decent work and economic growth, 9: industry innovation and infrastructure, 11: sustainable cities and communities, 12: responsible consumption and production, 13: climate action, 14: life below water, 17: partnerships for sustainable development. The theoretical basis of the research was scientific works of scholars from different countries and periods in the field of logistics, sustainable development, individual provisions of international framework documents, including the UN Conference on Environment and Development's Agenda for the 21st Century, the Declaration on Environmental Protection, and the Johannesburg Declaration on Sustainable Development. Official data from the ports of Gdansk, Rotterdam, and Hamburg, the International Association of Ports and Harbors (IAPH), the World Ports Climate Initiative (WPCI), the World Bank, and the Administration of Seaports of Ukraine (USPA) were used as initial information for analysis and concluding. As a result of summarizing the theoretical and methodological foundations of logistics, the article proposes the Conceptual Model of the Subject Area 'Smart Port', the quintessence of which lies in the symbiosis of the paradigms of scientific and technical development and sustainable development. This made it possible to expand the existing scientific basis of the transport logistics and transport systems sphere. The prerequisites for the emergence of the smart port are analyzed. The leading container ports of different generations are grouped. Special focus is on Ukraine, one of the largest grain exporters in the World. The prospects for the transition of Ukrainian ports to the fourth category (generation) of development are outlined. To successfully implement the strategy for the development of Ukrainian seaports for the period up to 2038, an optimization management model for logistics processes was developed based on the concept of a gaming approach.