bluuebunny/crossref_metadata_2025_split huggingface dataset:
Trends on Chlamydomonas reinhardtii growth regimes and bioproducts
Authors: Pessoa Jassiara da Silva , de Oliveira Caroline Frere Martiniuc , Mena‐Chalco Jesús Pascual , de Carvalho João Carlos Monteiro , Ferreira‐Camargo Livia Seno 
Published: June 2023

AbstractThe green microalga Chlamydomonas reinhardtii is a model microorganism for several areas of study. Among the different microalgae species, it presents advantageous characteristics, such as genomes completely sequenced and well‐established techniques for genetic transformation. Despite that, C. reinhardtii production is still not easily commercially viable, especially due to the low biomass yield. So far there are no reports of scientometric study focusing only on C. reinhardtii biomass production process. Considering the need for culture optimization, a scientometric research was conducted to analyze the papers that investigated the growth regimes effects in C. reinhardtii cultivation. The search resulted in 130 papers indexed on Web of Science and Scopus platforms from 1969 to December 2022. The quantitative analysis indicated that the photoautotrophic regime was the most employed in the papers. However, when comparing the three growth regimes, the mixotrophic one led to the highest production of biomass, lipids, and heterologous protein. The production of bioproducts was considered the main objective of most of the papers and, among them, biomass was the most frequently investigated. The highest biomass production reported among the papers was 40 g L−1 in the heterotrophic growth of a transgenic strain. Other culture conditions were also crucial for C. reinhardtii growth, for instance, temperature and cultivation process.
Potential of butanol production from Thailand marine macroalgae using Clostridium beijerinckii ATCC 10132‐based ABE fermentation
Authors: Khaonuan Sireethorn , Jariyaboon Rattana , Usmanbaha Nikannapas , Cheirsilp Benjamas , Birkeland Nils‐Kåre , Kongjan Prawit 
Published: June 2023

AbstractThe economical bio‐butanol‐based fermentation process is mainly limited by the high price of first‐generation biomass, which is an intensive cost for the pretreatment of second‐generation biomass. As third‐generation biomass, marine macroalgae could be potentially advantageous for conversion to clean and renewable bio‐butanol through acetone‐butanol‐ethanol (ABE) fermentation. In this study, butanol production from three macroalgae species (Gracilaria tenuistipitata, Ulva intestinalis, and Rhizoclonium sp.) by Clostridium beijerinckii ATCC 10132 was assessed comparatively. The enriched C beijerinckii ATCC 10132 inoculum produced a high butanol concentration of 14.07 g L−1 using 60 g L−1 of glucose. Among the three marine seaweed species, G. tenuistipitata exhibited the highest potential for butanol production (1.38 g L−1). Under the 16 conditions designed using the Taguchi method for low‐temperature hydrothermal pretreatment (HTP) of G. tenuistipitata, the maximum reducing sugar yield rate of 57.6% and ABE yield of 19.87% were achieved at a solid to liquid (S/L) ratio of 120, temperature of 110°C, and holding time of 10 min (Severity factor, R0 1.29). In addition, pretreated G. tenuistipitata could be converted to 3.1 g L−1 of butanol using low‐HTP at an S/L ratio of 50 g L−1, temperature of 80°C (R0 0.11), and holding time of 5 min.
Production of adeno‐associated viral vector serotype 6 by triple transfection of suspension HEK293 cells at higher cell densities
Authors: Moço Pablo D. , Xu Xingge , Silva Cristina A. T. , Kamen Amine A. 
Published: June 2023

AbstractIn recent years, the use of adeno‐associated viruses (AAVs) as vectors for gene and cell therapy has increased, leading to a rise in the amount of AAV vectors required during pre‐clinical and clinical trials. AAV serotype 6 (AAV6) has been found to be efficient in transducing different cell types and has been successfully used in gene and cell therapy protocols. However, the number of vectors required to effectively deliver the transgene to one single cell has been estimated at 106 viral genomes (VG), making large‐scale production of AAV6 necessary. Suspension cell‐based platforms are currently limited to low cell density productions due to the widely reported cell density effect (CDE), which results in diminished production at high cell densities and decreased cell‐specific productivity. This limitation hinders the potential of the suspension cell‐based production process to increase yields. In this study, we investigated the improvement of the production of AAV6 at higher cell densities by transiently transfecting HEK293SF cells. The results showed that when the plasmid DNA was provided on a cell basis, the production could be carried out at medium cell density (MCD, 4 × 106 cells mL−1) resulting in titers above 1010 VG mL−1. No detrimental effects on cell‐specific virus yield or cell‐specific functional titer were observed at MCD production. Furthermore, while medium supplementation alleviated the CDE in terms of VG/cell at high cell density (HCD, 10 × 106 cells mL−1) productions, the cell‐specific functional titer was not maintained, and further studies are necessary to understand the observed limitations for AAV production in HCD processes. The MCD production method reported here lays the foundation for large‐scale process operations, potentially solving the current vector shortage in AAV manufacturing.
Astaxanthin production from the microalga Haematococcus lacustris with a dual substrate mixotrophy strategy
Authors: Khazi Mahammed Ilyas , Liaqat Fakhra , Gu Wenhui , Mohamed Badr , Zhu Daochen , Li Jian 
Published: June 2023

AbstractThis study investigates the development of dual‐substrate mixotrophy strategy to cultivate the microalga Haematococcus lacustris for astaxanthin production. The influence of different concentrations of acetate and pyruvate on biomass productivity was first assessed individually, and then both substrates were used together to improve biomass growth in the green phase and astaxanthin accumulation in red the phase. The results showed that dual‐substrates mixotrophy significantly increased the biomass productivity during green growth phase up to 2‐fold compared to phototrophic controls. Furthermore, supplementation of dual‐substrate to the red phase increased astaxanthin accumulation by 10% in the dual‐substrate group compared to single‐substrate acetate and no substrate. This dual‐substrate mixotrophy approach shows promise for cultivating Haematococcus for commercial production of biological astaxanthin in indoor closed systems.
Quantum dots decorated photoanodes in bioelectrochemical fuel cells: Enhanced electricity generation using green algae
Authors: Cevik Emre , Gondal Mohammed A. , Alqahtani Noha , Hassan M. 
Published: June 2023

AbstractThe power performance of the bio‐electrochemical fuel cells (BEFCs) depends mainly on the energy harvesting ability of the anode material. The anode materials with low bandgap energy and high electrochemical stability are highly desirable in the BEFCs. To address this issue, a novel anode is designed using indium tin oxide (ITO) modified by chromium oxide quantum dots (CQDs). The CQDs were synthesized using facile and advanced pulsed laser ablation in liquid (PLAL) technique. The combination of ITO and CQDs improved the optical properties of the photoanode by exhibiting a broad range of absorption in the visible to UV region. A systematic study has been performed to optimize the amount of CQDs and green Algae (Alg) film grown using the drop casting method. Chlorophyll (a, b, and total) content of algal cultures (with different concentrations) were optimized to investigate the power generation performance of each cell. The BEFC cell (ITO/Alg10/Cr3//Carbon) with optimized amounts of Alg and CQDs demonstrated enhanced photocurrent generation of 120 mA cm−2 at a photo‐generated potential of 24.6 V m−2. The same device exhibited a maximum power density of 7 W m−2 under continuous light illumination. The device also maintained 98% of its initial performance after 30 repeated cycles of light on–off measurements.
“Biosynthesis of psilocybin and its nonnatural derivatives by a promiscuous psilocybin synthesis pathway in Escherichia coli”
Authors: Flower Jessica E. , Gibbons William J. , Adams Alexandra M. , Wang Xin , Broude Caroline N. , Jones J. Andrew 
Published: June 2023

AbstractTraditional psychedelics are undergoing a transformation from recreational drugs, to promising pharmaceutical drug candidates with the potential to provide an alternative treatment option for individuals struggling with mental illness. Sustainable and economic production methods are thus needed to facilitate enhanced study of these drug candidates to support future clinical efforts. Here, we expand upon current bacterial psilocybin biosynthesis by incorporating the cytochrome P450 monooxygenase, PsiH, to enable the de novo production of psilocybin as well as the biosynthesis of 13 psilocybin derivatives. The substrate promiscuity of the psilocybin biosynthesis pathway was comprehensively probed by using a library of 49 single‐substituted indole derivatives, providing biophysical insights to this understudied metabolic pathway and opening the door to the in vivo biological synthesis of a library of previously unstudied pharmaceutical drug candidates.
Buy green only: Interplay between green marketing, corporate social responsibility and green purchase intention; the mediating role of green brand image
Authors: Jabeen Riffut , Khan Kashif Ullah , Zain Fahad , Atlas Fouzia 
Published: June 2023

AbstractConsumers around the globe are concerned about the environment and want to purchase from businesses that are more concerned about society and the environment. Therefore, businesses need to develop their image as socially responsible entities and build their green image through green marketing tactics to enhance the green purchase intentions of their consumers. The study is quantitative, and a convenience sampling technique is used to collect primary data through a self‐administered questionnaire comprised of the demographic profile of the respondents and adopted scales of all the constructs of the study. The population of the study is students of different universities in the Punjab province of Pakistan. Statistical tests are performed with the help of SPSS_v.20 and PLS_v.4 (variance based) on 782 useable responses. Green marketing (GM) and corporate social responsibility (CSR) are the independent variables, while green brand image (GBI) is mediating, and green purchase intention (GPI) is the dependent variable of the study. The results indicate that GM and CSR improved the GBI and built GPI. This study contributes to the social and environmental literature. It may help managers to formulate strategies to enhance the Green Purchase Intentions of customers by building a Green Brand Image.
Anatomy of the Ventricular Outflow Tracts: An Electrophysiology Perspective
Authors: Haq Ikram U. , Shabtaie Samuel A. , Tan Nicholas Y. , Lachman Nirusha , Asirvatham Samuel J. 
Published: June 2023

AbstractOutflow tract ventricular arrhythmias are the most common type of idiopathic ventricular arrhythmia. A systematic understanding of the outflow tract anatomy improves procedural efficacy and enables electrophysiologists to anticipate and prevent complications. This review emphasizes the three‐dimensional spatial relationships between the ventricular outflow tracts using seven anatomical principles. In turn, each principle is elaborated on from a clinical perspective relevant for the practicing electrophysiologist. The developmental anatomy of the outflow tracts is also discussed and reinforced with a clinical case.
Polysaccharides and Bioactive Phenolics from Aconitum septentrionale Roots
Authors: Fu Yu‐Ping , Malterud Karl Egil , Hamre Anne Grethe , Inngjerdingen Kari Tvete , Wangensteen Helle 
Published: June 2023

AbstractAconitum septentrionale is known to contain toxic diterpene alkaloids, while other bioactive compounds in the plant remain unclear. The aim of this study was to explore the phenolic compounds and polysaccharides from the water extract of A. septentrionale roots. Fifteen phenolic compounds were isolated and identified by NMR and MS, including fourteen known and one new dianthramide glucoside (2‐[[2‐(β‐D‐glucopyranosyloxy)‐5‐hydroxybenzoyl]amino]‐4,5‐dihydroxybenzoic acid methyl ester, 14). One neutral (complex of glucans with minor amounts of mannans) and two acidic polysaccharide fractions (complexes of pectic polysaccharides and glucans) were also obtained. Hydroxytyrosol (1), hydroxytyrosol‐1‐O‐β‐glucoside (2) and bracteanolide A (7) inhibited the release of nitric oxide by dendritic cells. Magnoflorine (8) and 2‐[[2‐(β‐D‐glucopyranosyloxy)‐5‐hydroxybenzoyl]amino]‐5‐hydroxybenzoic acid methyl ester (12) inhibited 15‐lipoxygenase, and bracteanolide A (7) was a moderate inhibitor of xanthine oxidase. This study is the first to describe the diversity of phenolics and polysaccharides from A. septentrionale and their anti‐inflammatory and anti‐oxidant activities.
Phytoconstituents of Selaginella effusa Alston and Their α‐Glucosidase as well as Urease Inhibitory Activities
Authors: Sun Wentao , Luo Li , Luo Jiazi , Song Xixi , Tang Demin , Song Zishuo , Li Meishan , Wang Hengshan 
Published: June 2023

AbstractThree new compounds (1–2, 14), as well as 22 known compounds (3–13, 15–25), were extracted for the first time from the Selaginella effusa Alston (S. effusa). For the unknown compounds, the planar configurations were determined via NMR and by high‐resolution mass spectrometry, while their absolute configurations were determined by calculated electronic circular dichroism (ECD), and the configuration of the stereogenic center of biflavones 4–5 were established for the first time. The pure compounds (1–25) were tested in vitro to determine the inhibitory activity of the enzyme‐catalyzed reactions. Compounds 1–9 inhibited α‐glucosidase with IC50 values ranging from 0.30±0.02 to 4.65±0.04 μM and kinetic analysis of enzyme inhibition indicated that biflavones 1–3 were mixed‐type α‐glucosidase inhibitors. Compounds 12–13 showed excellent inhibitory activity against urease, with compound 12 (IC50=4.38±0.31 μM) showing better inhibitory activity than the positive control drug AHA (IC5013.52±0.61 μM). In addition, molecular docking techniques were used to simulate inhibitor‐enzyme binding and to estimate the binding posture of the α‐glucosidase and urease catalytic sites.
Renal clear cancer cells regulate the differentiation of urine‐derived stem cells to carcinoma‐associated fibroblasts via RUNX3/TGF‐β1 signaling axis
Authors: Chen Liang , Luo Shengjun , Gao Xiangna , Wang Delin 
Published: June 2023

AbstractClear cell renal cell carcinoma (ccRCC), the most common pathological subtype of renal cancer, is one of the significant health concerns due to limited clinically effective treatments. Nevertheless, targeting carcinoma‐associated fibroblasts in the tumor microenvironment has emerged as a promising innovative strategy for renal cancer therapy. Thus, this study is aimed to explore the role and molecular mechanism of urine‐derived stem cells (USCs) in the progression and metastasis of ccRCC. Initially, wound‐healing and transwell experiments were used to assess the migration and invasion abilities of the cells. Then, western blot analysis (WB) and quantitative reverse transcriptase‐polymerase chain reaction (qRT‐PCR) analyses were used to demonstrate the relevant protein and messenger RNA expression levels. Finally, hematoxylin–eosin and immunohistochemical stainings were performed to evaluate metastasis and protein expression in lung tumors. The coculture of USCs with the ccRCC cell lines significantly enhanced their migratory and invasive abilities. WB and qRT‐PCR analyses exhibited that ccRCC cell lines significantly increased cell mobility markers transcriptional and protein levels in USCs. Finally, the in vivo investigations in nude mice showed that USCs promoted the proliferation and migration of ccRCC‐based xenograft tumors. In summary, these findings demonstrated that USCs promoted ccRCC tumorigenesis and development in vivo and in vitro by regulating the Runt‐related transcription factor 3/transforming growth factor‐β1 signaling axis.
Low thermal conductivity lanthanum zirconate nanofibrous membranes for thermal insulation
Authors: Wang Hengchang , Xu Jie , Wei Mingyue , Meng Xuanyu , Lin Lang , Feng Xiaoying , Gao Feng 
Published: June 2023

AbstractLanthanum zirconate (LZO) has high‐temperature advantages, including a high melting point, low thermal conductivity, and high structure stability, which has the potential to be used in high‐temperature thermal insulation field. However, low mechanical properties of LZO porous ceramics have limited further applications. In this work, LZO nanofibrous membranes were prepared by electrospinning, and the preparation parameters, including PVP content, spinning voltage, feeding rate, received distance, and the heating process, were investigated to optimize the microstructure of the nanofibrous membranes. The LZO nanofibrous membranes with a grain size of 34.5 nm displayed good morphology after calcining at 1000°C. The LZO fibrous membranes had a very low thermal conductivity of 0.0716 W/m·K at room temperature, showing excellent insulating performance. This work indicates that LZO nanofibrous membranes are promising candidate materials for thermal insulation applications.
Structural Expansion of Cyclohepta[def]fluorene towards Azulene‐Embedded Non‐Benzenoid Nanographenes
Authors: Wu Fupeng , Barragán Ana , Gallardo Aurelio , Yang Lin , Biswas Kalyan , Écija David , Mendieta‐Moreno Jesús I. , Urgel José I. , Ma Ji , Feng Xinliang 
Published: June 2023

AbstractNon‐benzenoid non‐alternant nanographenes (NGs) have attracted increasing attention on account of their distinct electronic and structural features in comparison to their isomeric benzenoid counterparts. In this work, we present a series of unprecedented azulene‐embedded NGs on Au(111) during the attempted synthesis of cyclohepta[def]fluorene‐based high‐spin non‐Kekulé structure. Comprehensive scanning tunneling microscopy (STM) and non‐contact atomic force microscopy (nc‐AFM) evidence the structures and conformations of these unexpected products. The dynamics of the precursor bearing 9‐(2,6‐dimethylphenyl)anthracene and dihydro‐dibenzo‐cyclohepta[def]fluorene units and its reaction products on the surface are analyzed by density functional theory (DFT) and molecular dynamics (MD) simulations. Our study sheds light on the fundamental understanding of precursor design for the fabrication of π‐extended non‐benzenoid NGs on a metal surface.
A Blueprint for the Stabilization of Sub‐Valent Alkaline Earth Complexes**
Authors: Bowles Alex W. J. , Liu Yu , Stevens Matthew P. , Vitorica‐Yrezabal Iñigo J. , McMullin Claire L. , Ortu Fabrizio 
Published: June 2023

AbstractThe study of sub‐valent Group 2 chemistry is a relatively new research field, being established in 2007 with the report of the first Mg(I) dimers. These species are stabilized by the formation of a Mg−Mg covalent bond; however, the extension of this chemistry to heavier alkaline earth (AE) metals has been frustrated by significant synthetic challenges, primarily associated with the instability of heavy AE−AE interactions. Here we present a new blueprint for the stabilization of heavy AE(I) complexes, based upon the reduction of AE(II) precursors with planar coordination geometries. We report the synthesis and structural characterisation of homoleptic trigonal planar AE(II) complexes of the monodentate amides {N(SiMe3)2}− and {N(Mes)(SiMe3)}−. DFT calculations showed that the LUMOs of these complexes all show some d‐character for AE = Ca−Ba. DFT analysis of the square planar Sr(II) complex [Sr{N(SiMe3)2}(dioxane)2]∞ revealed analogous frontier orbital d‐character. AE(I) complexes that could be accessed by reduction of these AE(II) precursors were modelled computationally, revealing exergonic formation in all cases. Crucially, NBO calculations show that some d‐character is preserved in the SOMO of theoretical AE(I) products upon reduction, showing that d‐orbitals could play a crucial role in achieving stable heavy AE(I) complexes.
Effects of Metal Cations and Counter Anions on the Structural Stability of Isoquinoline‐Based Metallo‐Supramolecular Cages
Authors: Chen Zhi , Xie Yinghao , Wang Heng , Yu Xiujun , Li Xiaopeng 
Published: June 2023

Comprehensive SummaryMetallo‐supramolecular architectures that are constructed by coordination‐driven self‐assembly have received tremendous attention on account of their diverse yet molecular‐level precise structures and broad applications. Of particular, metal cations and counter anions are fundamentally important in terms of self‐assembly, characterization and property; however, their effects on the structural stabilities of metallo‐supramolecular architectures have seldom been investigated. To address this issue, herein, a series of octahedral metallo‐cages that are capable of tolerating with five metal cations (Pd2+, Cu2+, Ni2+, Co2+ and Zn2+), and five counter anions (ClO4–, OTf–, BF4–, NTf2– and NO3–) are constructed by the coordination‐driven self‐assembly of a well‐designed tritopic isoquinoline‐based ligand with corresponding metal salts. Structural stability studies show that metal cations and counter anions play a critical role in the stability of the resulting cages depending on their coordination abilities and stacking manners. This work provides deep insights in the ever‐diversifying field of metallo‐supramolecular chemistry, and will enable us to design more sophisticated assembled structure with desired function.
Binary Co‐Crystals of Quercetin: Synthesis, Structure, and Spectroscopic Characterization
Authors: Maciołek Urszula , Mendyk Ewaryst , Kuśmierz Marcin , Kozioł Anna E. 
Published: June 2023

AbstractA mechanochemical method was used to obtain four new quercetin (QUE) co‐crystals. Three co‐formers have the systems of the heterocyclic rings with the oxygen and nitrogen atoms and they form co‐crystals at the stoichiometric ratio of 1 : 2. In contrast, the QUE : o‐dianisidine co‐crystal represents the 1 : 1 stoichiometry and the former molecule is the aniline derivative. The X‐ray crystallography and FT‐IR and FT‐Raman spectra revealed formation of the intermolecular O−H…N or N−H…O hydrogen bonds. The dynamics of the hydrogen bonds was investigated using the XPS method. The N 1s XPS spectra showed no proton transfer in the QUE : FEN and QUE : O‐DIA co‐crystal systems. The QUE : BZFP and QUE : EBZFP show the two‐site static disorder across the proton transfer pathway to the pyridine ring, with the occupancies (C=N : C=NH+) of 72 : 28 and 77 : 23, respectively.