bluuebunny/crossref_metadata_2025_split huggingface dataset:
Characterization of Turkish pine honey according to their geographical origin based on physicochemical parameters and chemometrics
Authors: Uçurum H. Özgül , Tepe Şeref , Yeşil Ersel , Güney Fazıl , Karakuş Sümeyye , Kolayli Sevgi , Takma Çiğdem , Duru M. Emin , Özkök Aslı , Yücel Banu , Karaca Üzeyir , Sorkun Kadriye , Baran Aynur , Kilinç Ahmet , Köseoğlu Mustafa , Özsoy Neslihan , Kunduraci B. Sami , Türkaslan Nurten , Atmaca Hilal , Karaağaç Hacer E. , Topal Erkan , Dayioğlu Miray , Kalkan Mücait , Ekerbiçer Ünal , Çakici Neslihan 
Published: March 2023

AbstractThis study was conducted to determine the characteristic properties of Turkish pine honey, which is an important honeydew honey. The geographical classification of the honey was determined by applying carbon isotope, melissopalynological, and physicochemical analyses to 373 samples collected from 47 regions between 2015 and 2017 under controlled conditions. δ13C protein-δ13Choney, C4%, electrical conductivity, moisture, ash, free acidity, color CIEL* a*b attributes, optical rotation [α]20, proline, diastases activities, and sugars (fructose, glucose, sucrose, and maltose) were used as physicochemical properties. Number of honeydew elements /number of total pollen (NHE/NTP) ratios were studied at melissopalynological analyses. The results showed that all samples exhibited honeydew properties, and that all physicochemical parameters met the criteria set by regulatory standards for honeydew. However, C4% sugar and δ13C protein-δ13C honey values did not meet the regulatory criteria and exhibited quite wide standard deviations.
Accuracy of CT Scan for Detecting Hollow Viscus Injury in Penetrating Abdominal Trauma
Authors: Wolmarans Anika , Fru Pascaline N. , Moeng Maeyane S. 
Published: March 2023

AbstractBackgroundIn penetrating abdominal trauma, computed tomography (CT) is routinely performed to evaluate stable patients for selective non‐operative management (SNOM). Triple‐contrast CT (oral, rectal, and IV) has traditionally been used. However, due to its disadvantages, most trauma centres, including our unit at the Charlotte Maxeke Johannesburg Academic Hospital (CMJAH), now perform single‐contrast intravenous‐only CT scans. We performed a retrospective review to determine the accuracy of single‐contrast CT scans for detecting hollow viscus injuries (HVI) in penetrating abdominal trauma.MethodsA retrospective review of all patients who presented to CMJAH with penetrating abdominal injuries was performed between 01 August 2017 and 31 August 2019 and were evaluated for SNOM with CT (IV contrast only). Patient records were reviewed to determine pertinent demographics, mechanism, and site of injury, as well as metabolic parameters. CT findings were compared to findings at laparotomy.ResultsA total of 437 patients met the inclusion criteria. The majority were male (92.7%), with a mean age of 31.5 yrs (SD 8.7). Injuries were predominantly due to stab wounds (72,5%, n = 317). CT scan was negative in 342 patients, of which 314 completed SNOM successfully. A total of 93 patients proceeded to laparotomy. CT had a sensitivity of 95.1%, specificity of 44.2%, positive predictive value of 57.4%, and negative predictive value of 92%.ConclusionSingle‐contrast CT in penetrating abdominal trauma is a valuable investigative tool in identifying patients for SNOM. Features of HVI on single‐contrast CT are not very specific and should be interpreted along with other clinical factors including wound trajectory and serial abdominal examinations. Other associated injuries such as diaphragmatic and solid organ injuries should be considered in the final management plan.
Apremilast for biologic-naïve, peripheral psoriatic arthritis, including patients with early disease: results from the APROACH observational prospective study
Authors: Sfikakis Petros P. , Vassilopoulos Dimitrios , Katsifis Gkikas , Vosvotekas Georgios , Dimitroulas Theodoros , Sidiropoulos Prodromos , Vounotrypidis Periklis , Bogdanos Dimitrios P. , Georgountzos Athanasios Ι. , Bounas Andreas G. , Georgiou Panagiotis , Gazi Souzana , Kataxaki Evangelia , Liossis Stamatis-Nick , Theodorou Evangelos , Papagoras Charalampos , Theotikos Evangelos , Vlachoyiannopoulos Panayiotis , Voulgari Paraskevi V. , Kekki Angeliki , Antonakopoulos Nikolaos , Boumpas Dimitrios T. 
Published: March 2023

AbstractTo evaluate the effect of the phosphodiesterase 4 inhibitor apremilast in biologic-naïve patients with early peripheral PsA in terms of disease activity, clinical manifestations, patient-perceived outcomes, as well as apremilast’s safety profile in routine care settings of Greece. Non-interventional, multicenter, 52-week prospective cohort study, enrolling biologic-naïve patients with early active peripheral PsA who started apremilast after intolerance or inadequate response (within the first 12 months of treatment) to an initial conventional synthetic (cs)DMARD treatment. Non-responder imputation was applied for missing data.In total, 167 consecutive patients (mean age: 52.5 years; median PsA duration: 0.9 years) were analyzed. At baseline, the median (interquartile range) clinical Disease Activity in Psoriatic Arthritis (cDAPSA) score was 22.0 (16.0–29.0), with 86.8% of patients having at least moderate (29.3% high) disease activity; 87.4% had skin psoriasis, 37.7% nail psoriasis, 30.7% enthesitis, and 12.4% dactylitis. At 16, 24, and 52 weeks, 28.7, 42.5, and 48.5% of patients, achieved ≥ 50% improvement in their baseline cDAPSA score, respectively. At week 52, 55.6, 50, and 26.8% of evaluable patients achieved complete resolution of enthesitis, dactylitis and nail psoriasis, respectively. Improvements were also observed in patient’s health state assessed by the Psoriatic Arthritis Impact of Disease 12-item questionnaire, and health-related quality of life. The 52-week drug survival rate was 75%, while 13.8% of patients experienced at least one adverse drug reaction.Biologic-naïve patients with early PsA, treated with apremilast experienced significant improvements in disease activity, extra-articular manifestations and patient-centered outcomes, accompanied by a favorable tolerability profile.
Seaweed Extract Biostimulants Differentially act in Mitigating Drought Stress on Faba Bean (Vicia faba L.)
Authors: El Boukhari Mohammed El Mehdi , Barakate Mustapha , Drissi BadrEddine , Bouhia Youness , Lyamlouli Karim 
Published: March 2023

AbstractProlonged drought periods cause a cascade of biochemical alterations in plants and lead to reduction in plant growth and crop productivity. Seaweed extracts are a category of plant biostimulants that are effective in alleviating drought stress on plants. However, the effect of seaweed extracts on attenuating the negative impact of drought on Faba bean (Vicia faba L.) under arid and semi-arid conditions has received little attention. This study was conducted to evaluate the performances of extracts made from Fucus spiralis (FSE), Ulva lactuca (ULE), Laminaria ochroleuca (LOE), and Ascophyllum nodosum (ANE) in mitigating drought stress in Faba bean. The biochemical profile of the extracts was characterized, and key physiological and biochemical parameters of Faba bean plants were assessed during both drought and recovery phases. All investigated extracts positively affected plant biomass under drought stress conditions. Plants that received LOE had a higher relative water content and lower malondialdehyde concentration in comparison with stressed control plants. The positive effect of ANE and FSE was mainly attributed to proline accumulation in plant tissues under stress. FSE and ULE application resulted in a higher concentration of soluble sugars in treated plants in comparison to the control plants. It was concluded that seaweed extracts originating from different sources and extracted using different protocols act differentially in altering plant-related stress traits. Overall, seaweed extracts are potentially an effective solution to manage the negative impact of water scarcity on Faba bean.
Aerodynamic optimization of a generic light truck under unsteady conditions using gradient-enriched machine learning control
Authors: Semaan Richard , Oswald Philipp , Cornejo Maceda Guy Y. , Noack Bernd R. 
Published: March 2023

AbstractWe present the first machine-learned multiple-input multiple-output aerodynamic feedback control under varying operating conditions. Closed-loop control is relevant to many fluid dynamic applications ranging from gust mitigation to drag reduction. Existing machine learning control investigations have been mainly applied under steady conditions. The current study leverages gradient-enriched machine learning control (Cornejo Maceda et al. in J Fluid Mech 917:A42, 2021) to identify optimal control laws under unsteady conditions. The approach is exemplified on a coupled oscillator system with unsteady coupling and demonstrated for a generic truck model undergoing a yawing maneuver. Key enablers of the experiment are a rich set of pneumatic actuators and pressure sensors. The results demonstrate the method’s capabilities in identifying an efficient forcing for control under dynamically changing conditions. This automated and generalizable closed-loop control strategy complements and expands the machine learning control field and promises a new fast-track avenue to efficiently control a broader set of fluid flow problems.
The relationship between hearing status, listening effort, and the need for recovery in employees of a manufacturing company
Authors: van der Hoek-Snieders Hanneke E. M. , de Laat Jan A. P. M. , Dreschler Wouter A. 
Published: March 2023

Abstract
Objective
Hearing screening can be used to detect hearing loss, but its value for identifying employees with work functioning difficulties is unclear. The objective of this study was to assess the association between the hearing status measured with an occupational hearing-in-noise screening test, Listening Effort (LE), and Need For Recovery (NFR) in employees of a manufacturing company, and to examine whether these associations depend on the perceived noise level at the workplace.

Methods
Employees of coatings and paints manufacturing company were included. Their hearing status was assessed with an occupational hearing-in-noise screening test. An online survey was used to assess their LE, NFR, and the perceived noise level at the workplace. Responses from 143 employees were analyzed (mean age = 53 years) using hierarchical multiple regression analysis with the outcomes LE and NFR.

Results
Regression analysis—with adjustments for gender, age, educational level, health status, pace/amount of work, job variety, and work pleasure—revealed that hearing status was significantly associated with LE, but the interaction between hearing status and the perceived noise level was not. Hearing status nor the interaction between hearing status and the perceived noise level was significantly associated with NFR.

Conclusion
The results confirm that poorer hearing is associated with higher LE, but not with higher NFR. These associations were unrelated to the perceived noise level at the workplace. Therefore, the value of occupational hearing screening appears to be early identification of hearing loss in employees, but not identification of work functioning difficulties.
Oculomotor fatigability with decrements of saccade and smooth pursuit for diagnosis of myasthenia gravis
Authors: Nguyen Thanh Tin , Kang Jin-Ju , Chae Ju-Hee , Lee Eunsu , Kim Hyo-Jeong , Kim Ji-Soo , Oh Sun-Young 
Published: March 2023

AbstractBackground and objectivesAs the efficacy of current diagnostic methods for myasthenia gravis (MG) remains suboptimal, there is ongoing interest in developing more effective diagnostic models. As oculomotor fatigability is one of the most common and diagnostic symptoms in MG, we aimed to investigate whether quantitative saccadic and smooth-pursuit fatigability analyses with video-oculography (VOG) are useful for diagnosis of MG.MethodsA convenience cohort of 46 MG patients was recruited prospectively, including 35 with ocular and 11 with generalized MG (mean age, 50.9 ± 14.5 years; 17 females); 24 healthy controls (HCs) (mean age, 50.6 ± 16.3 years; 13 females) also were enrolled. Seventy-five repetitive saccades and smooth pursuits were recorded in ranges of 20° (horizontal plane) and 15° (vertical plane) using a three-dimensional VOG system. Based on the oculomotor range of the second saccade and smooth pursuit and the mean ranges of the last five of each, the estimated decrements (%) reflecting oculomotor fatigability were calculated.ResultsThe baseline oculomotor ranges did not show significant difference between the MG and HCs groups. However, following repetitive saccades and pursuits, the oculomotor ranges were decreased substantially during the last five cycles compared to baseline in the MG group. No such decrements were observed in the HC group (p < 0.01, Mann–WhitneyUtest). Receiver operating characteristic (ROC) analysis revealed that repetitive vertical saccades yielded the best differentiation between the MG and HC groups, with a sensitivity of 78.3% and specificity of 95.8% when using a decrement with an amplitude of 6.4% as the cutoff.ConclusionThis study presents an objective and reproducible method for measuring decrements of oculomotor ranges after repetitive saccadic and pursuit movements. Quantification of oculomotor fatigability using VOG could be a sensitive and specific diagnostic tool for MG and allows easy, cost-effective, accurate, and non-invasive measurements.Classification of evidenceThis study provides class III evidence that VOG-based quantification of saccadic and pursuit fatigability accurately identifies patients with MG.
Reduced cerebello-cerebral functional connectivity correlates with disease severity and impaired white matter integrity in Friedreich ataxia
Authors: Kerestes Rebecca , Cummins Hannah , Georgiou-Karistianis Nellie , Selvadurai Louisa P. , Corben Louise A. , Delatycki Martin B. , Egan Gary F. , Harding Ian H. 
Published: March 2023

AbstractFriedreich ataxia (FRDA) is a rare, inherited neurodegenerative disease characterised in most cases by progressive and debilitating motor dysfunction. Degeneration of cerebellar white matter pathways have been previously reported, alongside indications of cerebello-cerebral functional alterations. In this work, we examine resting-state functional connectivity changes within cerebello-cerebral circuits, and their associations with disease severity (Scale for the Assessment and Rating of Ataxia [SARA]), psychomotor function (speeded and paced finger tapping), and white matter integrity (diffusion tensor imaging) in 35 adults with FRDA and 45 age and sex-matched controls. Voxel-wise seed-based functional connectivity was assessed for three cerebellar cortical regions (anterior lobe, lobules I-V; superior posterior lobe, lobules VI-VIIB; inferior posterior lobe, lobules VIIIA-IX) and two dentate nucleus seeds (dorsal and ventral). Compared to controls, people with FRDA showed significantly reduced connectivity between the anterior cerebellum and bilateral pre/postcentral gyri, and between the superior posterior cerebellum and left dorsolateral PFC. Greater disease severity correlated with lower connectivity in these circuits. Lower anterior cerebellum-motor cortex functional connectivity also correlated with slower speeded finger tapping and less fractional anisotropy in the superior cerebellar peduncles, internal capsule, and precentral white matter in the FRDA cohort. There were no significant between-group differences in inferior posterior cerebellar or dentate nucleus connectivity. This study indicates that altered cerebello-cerebral functional connectivity is associated with functional status and white matter damage in cerebellar efferent pathways in people with FRDA, particularly in motor circuits.
Muscle strength and activity in men and women performing maximal effort biceps curl exercise on a new machine that automates eccentric overload and drop setting
Authors: Nuzzo James L. , Pinto Matheus D. , Nosaka Kazunori 
Published: March 2023

Abstract
Purpose
Connected adaptive resistance exercise (CARE) machines are new equipment purported to adjust resistances within and between repetitions to make eccentric (ECC) overload and drop sets more feasible. Here, we examined muscle strength, endurance, electromyographic activity (EMG), and perceptions of fatigue during unilateral bicep curl exercise with a CARE machine and dumbbells. We also tested for sex differences in muscle fatigability.

Methods
Twelve men and nine women attempted 25 consecutive coupled maximal ECC–concentric (CON) repetitions (ECCmax–CONmax) on a CARE machine. Participants also completed a CON one repetition maximum (1RM) and repetitions-to-failure tests with 60 and 80% 1RM dumbbells.

Results
Maximal strength on the CARE machine was greater during the ECC than CON phase, illustrating ECC overload (men: 27.1 ± 6.8, 14.7 ± 2.0 kg; women: 16.7 ± 4.7, 7.6 ± 1.4 kg). These maximal resistances demanded large neural drive. Biceps brachii EMG amplitude relative to CON dumbbell 1RM EMG was 140.1 ± 40.2% (ECC) and 96.7 ± 25.0% (CON) for men and 165.1 ± 61.1% (ECC) and 89.4 ± 20.4% (CON) for women. The machine’s drop setting algorithm permitted 25 consecutive maximal effort repetitions without stopping. By comparison, participants completed fewer repetitions-to-failure with the submaximal dumbbells (e.g., 60%1RM—men: 12.3 ± 4.4; women: 15.6 ± 4.7 repetitions). By the 25th CARE repetition, participants reported heightened biceps fatigue (~ 8 of 10) and exhibited large decreases in ECC strength (men: 63.5 ± 11.6%; women: 44.1 ± 8.0%), CON strength (men: 77.5 ± 6.5%; women: 62.5 ± 12.8%), ECC EMG (men: 38.6 ± 20.4%; women: 26.2 ± 18.3%), and CON EMG (men: 36.8 ± 20.4%; women: 23.1 ± 18.4%).

Conclusion
ECC overload and drop sets occurred automatically and feasibly with CARE technology and caused greater strength and EMG loss in men than women.
Characterization of POLE c.1373A > T p.(Tyr458Phe), causing high cancer risk
Authors: Rocque Mariève J. , Leipart Vilde , Kumar Singh Ashish , Mur Pilar , Olsen Maren F. , Engebretsen Lars F. , Martin-Ramos Edgar , Aligué Rosa , Sætrom Pål , Valle Laura , Drabløs Finn , Otterlei Marit , Sjursen Wenche 
Published: March 2023

AbstractThe cancer syndrome polymerase proofreading-associated polyposis results from germline mutations in the POLE and POLD1 genes. Mutations in the exonuclease domain of these genes are associated with hyper- and ultra-mutated tumors with a predominance of base substitutions resulting from faulty proofreading during DNA replication. When a new variant is identified by gene testing of POLE and POLD1, it is important to verify whether the variant is associated with PPAP or not, to guide genetic counseling of mutation carriers. In 2015, we reported the likely pathogenic (class 4) germline POLE c.1373A > T p.(Tyr458Phe) variant and we have now characterized this variant to verify that it is a class 5 pathogenic variant. For this purpose, we investigated (1) mutator phenotype in tumors from two carriers, (2) mutation frequency in cell-based mutagenesis assays, and (3) structural consequences based on protein modeling. Whole-exome sequencing of two tumors identified an ultra-mutator phenotype with a predominance of base substitutions, the majority of which are C > T. A SupF mutagenesis assay revealed increased mutation frequency in cells overexpressing the variant of interest as well as in isogenic cells encoding the variant. Moreover, exonuclease repair yeast-based assay supported defect in proofreading activity. Lastly, we present a homology model of human POLE to demonstrate structural consequences leading to pathogenic impact of the p.(Tyr458Phe) mutation. The three lines of evidence, taken together with updated co-segregation and previously published data, allow the germline variant POLE c.1373A > T p.(Tyr458Phe) to be reclassified as a class 5 variant. That means the variant is associated with PPAP.
Early postoperative gain in activity levels of lower extremity sarcoma survivors positively affects long-term physical activity and performance
Authors: Döring Kevin , Hegelmaier Anna Vanessa , Trost Carmen , Krall Christoph , Windhager Reinhard , Hobusch Gerhard Martin 
Published: March 2023

Abstract
Purpose
Little is known about the effect of sports activity levels on health-related quality of life (HRQOL) in long-term survivors of lower-extremity sarcoma.

Methods
Eighty-three long-term survivors of bone and soft tissue sarcoma of the lower extremities with a median follow-up of 14 (range: 5–35) years completed the University of California and Los Angeles (UCLA) activity scores before tumor resection, 1 year after surgery and at the latest follow-up, as well as a Short Form 36 (SF-36) health survey at the latest follow-up. Simple linear regression models as well as stepwise variable selection with Akaike information criterion (AIC) were undertaken.

Results
The preoperative UCLA activity level (median: 9, range: 2–10) dropped to a median of 4 (range: 1–10) 1 year after surgery before increasing to a score of 6 (range: 2–10) 5 years after surgery. The long-term SF-36 physical health component summary score (PCS) was 49 (SD: 9), and the mental health component summary score (MCS) was 54 (SD: 7). A linear model with stepwise variable selection identified a negative correlation of PCS with age at surgery (estimate: –0.2; p = 0.02), UCLA score at the last follow-up (estimate: 1.4; p = 0.02) and UCLA score 1 year after surgery (estimate: 1.0; p = 0.02).

Conclusion
As not only the final activity levels but also the status immediately after surgery affect the PCS, higher early activity levels should be a goal of modern rehabilitation after sarcoma treatment. Further studies are needed to weigh the potential postoperative risks of higher sport activity levels against the benefits described in this study.
Level of evidence: Level 4.
Diagnostic criteria and endoscopic and histological findings of autoimmune gastritis in Japan
Authors: Kamada Tomoari , Watanabe Hidenobu , Furuta Takahisa , Terao Shuichi , Maruyama Yasuhiko , Kawachi Hiroshi , Kushima Ryoji , Chiba Tsutomu , Haruma Ken 
Published: March 2023

AbstractThe Japanese diagnostic criteria for autoimmune gastritis (AIG) were established by the “Study Group on the establishment of diagnostic criteria for type A gastritis,” which is related to a workshop associated with the Japan Gastroenterological Endoscopy Society (JGES) and the Committee of AIG Research Group (CARP). The criteria were set as follows: the cases of confirmed diagnosis are patients in whom either the endoscopic or histological findings, or both, meet the requirements for AIG and who are confirmed to be positive for gastric autoantibodies (either anti-parietal cell or anti-intrinsic factor antibodies, or both). The presentation of endoscopic findings of early-stage AIG in the diagnostic criteria was withheld owing to the need for further accumulation and characterization of endoscopic clinical data. Therefore, diagnosis of early-stage AIG only requires histological confirmation and gastric autoantibody positivity. Suspected cases are patients in whom either the endoscopic or histological findings, or both, meet only the requirements for AIG. Histological findings only meet the requirements for early stage. AIG has been underdiagnosed in the past, but our study group’s newly proposed diagnostic criteria will enable a more accurate and early diagnosis of AIG. The criteria can be used to stratify patients into various high-risk groups for gastric tumors and pernicious anemia. They would allow the establishment of an appropriate surveillance system in the coming years. Nevertheless, issues such as establishing the endoscopic findings of early-stage AIG and obtaining Japanese insurance coverage for gastric autoantibody tests require attention.