bluuebunny/crossref_metadata_2025_split huggingface dataset:
Normal congruency sequence effects in psychopathology: A behavioral and electrophysiological examination using a confound‐minimized design
Authors: Clayson Peter E. , Shuford John L. , Rast Philippe , Baldwin Scott A. , Weissman Daniel H. , Larson Michael J. 
Published: September 2023

AbstractClinical studies of adaptive control emphasize the role disruptions in control play in psychopathology. However, many studies used confound‐laden designs and examined only one type of psychopathology. Recent studies of event‐related potentials (ERPs) suggest that robust congruency sequence effects (CSEs)—a popular index of adaptive control—appear in confound‐minimized designs. Thus, the present study sought to determine whether a confound‐minimized CSE paradigm could identify adaptive control dysfunction in people with major depressive disorder (MDD), generalized anxiety disorder (GAD), and obsessive‐compulsive disorder (OCD). We predicted that participants with MDD and GAD would show smaller ERP CSEs and that participants with OCD would show larger ERP CSEs than healthy controls. Data from 44 people with GAD, 51 people with MDD, 31 people with OCD, and 56 healthy comparison participants revealed normal CSEs as indexed by response times (RTs) and ERPs in the psychopathology groups. Moreover, psychiatric symptoms did not moderate these CSEs. Finally, we observed a strong mean–variance relationship in RT CSEs, such that participants with stronger post‐recruitment of control in mean RT scores showed the most consistent post‐conflict responses (i.e., the least intraindividual variability). These findings suggest that prior findings from confound‐laden tasks indicating altered CSEs in psychopathology stem from processes that are unrelated to adaptive control. Future research should employ experimental designs that isolate these processes to advance our understanding of abnormal CSEs in psychopathology.
Testosterone supplementation improves estrogen synthesis of buffalo (Bubalus bubalis) granulosa cells
Authors: Zhang Jun , Sun Junming , Xiao Linlin , Ouyang Yiqiang , Shi Deshun , Lu Fenghua 
Published: September 2023

AbstractGranulosa cells (GCs) synthesize estrogens needed for follicular growth. However, the effects of androgen on estrogen production in buffalo GCs remain unclear. In this study, the impacts of testosterone on estrogen synthesis in buffalo GCs were examined. The results showed that testosterone that was added to cell medium at a concentration of 10−7 mol/L and applied to GCs for 48 or 72 h enhanced the estrogen synthesis of buffalo GCs. This study provides a theoretical basis for further exploration of ovarian endocrine mechanism for steroidogenesis.
Quantifying demands on the hamstrings during high‐speed running: A systematic review and meta‐analysis
Authors: McNally Timothy , Edwards Suzi , Halaki Mark , O'Dwyer Nicholas , Pizzari Tania , Blyton Sarah 
Published: September 2023

AbstractIntroductionHamstring strain injury (HSI) remains a performance, economic, and player availability burden in sport. High‐speed running (HSR) is cited as a common mechanism for HSI. While evidence exists regarding the high physical demands on the hamstring muscles in HSR, meta‐analytical synthesis of related activation and kinetic variables is lacking.MethodsA systematic search of Medline, Embase, Scopus, CINAHL, SportDiscus, and Cochrane library databases was conducted in accordance with the PRISMA 2020 guidelines. Studies reporting hamstring activation (electromyographic [EMG]) or hamstring muscle/related joint kinetics were included where healthy adult participants ran at or beyond 60% of maximum speed (activation studies) or 4 m per second (m/s) (kinetic studies).ResultsA total of 96 studies met the inclusion criteria. Run intensities were categorized as “slow,” “moderate,” or “fast” in both activation and kinetic based studies with appropriate relative, and raw measures, respectively. Meta‐analysis revealed pooled mean lateral hamstring muscle activation levels of 108.1% (95% CI: 84.4%–131.7%) of maximal voluntary isometric contraction (MVIC) during “fast” running. Meta‐analysis found swing phase peak knee flexion internal moment and power at 2.2 Newton meters/kilogram (Nm/kg) (95% CI: 1.9–2.5) and 40.3 Watts/kilogram (W/kg) (95% CI: 31.4–49.2), respectively. Hip extension peak moment and power was estimated as 4.8 Nm/kg (95% CI: 3.9–5.7) and 33.1 W/kg (95% CI: 17.4–48.9), respectively.ConclusionsAs run intensity/speed increases, so do the activation and kinetic demands on the hamstrings. The presented data will enable clinicians to incorporate more objective measures into the design of injury prevention and return‐to‐play decision‐making strategies.
Transfusion of red blood cells in venoarterial extracorporeal membrane oxygenation: A multicenter retrospective observational cohort study
Authors: Raasveld Senta Jorinde , Karami Mina , Schenk Jimmy , Dos Reis Miranda Dinis , Mandigers Loes , Dauwe Dieter F. , De Troy Erwin , Pappalardo Federico , Fominskiy Evgeny , van den Bergh Walter M. , Oude Lansink‐Hartgring Annemieke , van der Velde Franciska , Maas Jacinta J. , van de Berg Pablo , de Haan Maarten , Donker Dirk W. , Meuwese Christiaan L. , Taccone Fabio Silvio , Peluso Lorenzo , Lorusso Roberto , Delnoij Thijs S. R. , Scholten Erik , Overmars Martijn , Ivančan Višnja , Bojčić Robert , de Metz Jesse , van den Bogaard Bas , de Bakker Martin , Reddi Benjamin , Hermans Greet , Broman Lars Mikael , Henriques José P. S. , Vlaar Alexander P. J. 
Published: September 2023

AbstractBackgroundEvidence‐based recommendations for transfusion in patients with venoarterial extracorporeal membrane oxygenation (VA ECMO) are scarce. The current literature is limited to single‐center studies with small sample sizes, therefore complicating generalizability. This study aims to create an overview of red blood cell (RBC) transfusion in VA ECMO patients.MethodsThis international mixed‐method study combined a survey with a retrospective observational study in 16 centers. The survey inventoried local transfusion guidelines. Additionally, retrospective data of all adult patients with a VA ECMO run >24 h (January 2018 until July 2019) was collected of patient, ECMO, outcome, and daily transfusion parameters. All patients that received VA ECMO for primary cardiac support were included, including surgical (i.e., post‐cardiotomy) and non‐surgical (i.e., myocardial infarction) indications. The primary outcome was the number of RBC transfusions per day and in total. Univariable logistic regressions and a generalized linear mixed model (GLMM) were performed to assess factors associated with RBC transfusion.ResultsOut of 419 patients, 374 (89%) received one or more RBC transfusions. During a median ECMO run of 5 days (1st–3rd quartile 3–8), patients received a median total of eight RBC units (1st–3rd quartile 3–17). A lower hemoglobin (Hb) prior to ECMO, longer ECMO‐run duration, and hemorrhage were associated with RBC transfusion. After correcting for duration and hemorrhage using a GLMM, a different transfusion trend was found among the regimens. No unadjusted differences were found in overall survival between either transfusion status or the different regimens, which remained after adjustment for potential confounders.ConclusionRBC transfusion in patients on VA ECMO is very common. The sum of RBC transfusions increases rapidly after ECMO initiation, and is dependent on the Hb threshold applied. This study supports the rationale for prospective studies focusing on indications and thresholds for RBC transfusion.
The effect of increased collect pump rate on collection efficiency in hematopoietic progenitor cell collection by apheresis in allogeneic adult donors—A single center analysis
Authors: Miller Anthony , Davies Jeramy , Young Kathryn , Eckman Emily , Lo Melissa Y. , Erskine Hannah , Knutson Lisa , Ondricek Sara , Margolis Jamie M. , Auletta Jeffery J. , Miller John P. , Stefanski Heather E. , Devine Steven , Pham Huy P. 
Published: September 2023

AbstractBackgroundOptimizing CD34 recovery while minimizing harm to hematopoietic progenitor cell donors by apheresis (HPC(A) donors) is critical to the success of allogeneic hematopoietic cell transplantation. We examined the efficacy and safety of starting allogeneic HPC(A) donors at a collect pump rate (CPR) of 2 mL/min on the Spectra Optia regardless of the inlet flow rate and/or pre‐apheresis white blood cell (WBC) count (high CPR group).Study Design and MethodsA single‐center retrospective study was performed on allogeneic adult donors from 10/2020 to 12/2022. From 10/2020 to 6/19/2022, all donors had CPR of ~1 mL/min (historical group). High CPR group started 6/20/2022.ResultsDuring the study period, 412 donors were in historical group versus 196 (32.2%) in high CPR group. Median CD34 collection efficiency (CE) was higher and more consistent in high CPR group (55.1% vs. 53% in historical group, p < .0001) and remained significant in multivariate analysis. Although product volume was higher in high CPR group, WBC, hematocrit, and platelet concentrations were significantly lower. No difference in engraftment outcomes in patients receiving products from two groups was observed. Moreover, no differences occurred in a significant peri‐procedural adverse event or percent decrease in platelets (6.87% decrease in platelets per 100 × 106 CD34 cells collected versus 6.66% in historical group, p = .89). Furthermore, high CPR group had ~26 min less in collection time for every 100 × 106 CD34 cells collected, resulting in less positive fluid balances.ConclusionsStarting allogeneic HPC(A) donor collection at a CPR of 2 mL/min is safe and effective.
A small cusped hyperbolic 4‐manifold
Authors: Riolo Stefano 
Published: September 2023

AbstractBy gluing some copies of a polytope of Kerckhoff and Storm's, we build the smallest known orientable hyperbolic 4‐manifold that is not commensurable with the ideal 24‐cell or the ideal rectified simplex. It is cusped and arithmetic, and has twice the minimal volume.
Equivariant knots and knot Floer homology
Authors: Dai Irving , Mallick Abhishek , Stoffregen Matthew 
Published: September 2023

AbstractWe define several equivariant concordance invariants using knot Floer homology. We show that our invariants provide a lower bound for the equivariant slice genus and use this to give a family of strongly invertible slice knots whose equivariant slice genus grows arbitrarily large, answering a question of Boyle and Issa. We also apply our formalism to several seemingly nonequivariant questions. In particular, we show that knot Floer homology can be used to detect exotic pairs of slice disks, recovering an example due to Hayden, and extend a result due to Miller and Powell regarding stabilization distance. Our formalism suggests a possible route toward establishing the noncommutativity of the equivariant concordance group.
Impact of acquired broad-spectrum β-lactamases on susceptibility to oral penems/carbapenems (tebipenem, sulopenem, and faropenem) alone or in combination with avibactam and taniborbactam β-lactamase inhibitors in
Escherichia coli
Authors: Le Terrier Christophe , Nordmann Patrice , Bouvier Maxime , Poirel Laurent 
Published: September 2023

ABSTRACT

The impact of β-lactamases on susceptibility to oral penems/carbapenems (tebipenem, sulopenem, and faropenem) and other carbapenem molecules was evaluated in
Escherichia coli
, alone and in combination with avibactam or taniborbactam β-lactamase inhibitors. Tebipenem and sulopenem exhibited a similar spectrum of activity compared to the intravenous carbapenems and displayed lower MIC values than ceftibuten-avibactam against
E. coli
producing extended-spectrum β-lactamases or AmpC enzymes. Combined with taniborbactam, tebipenem and sulopenem exhibited low MIC values against almost all tested recombinant
E. coli
, including metallo-β-lactamase producers.
Complete genome sequence of
Butyricimonas faecihominis
JCM 18676
T
Authors: Fukuoka Hironori , Tourlousse Dieter M. , Hamajima Mayu , Koike Kazuyoshi , Endo Itaru , Sekiguchi Yuji 
Published: September 2023

ABSTRACT

We report a complete genome sequence of
Butyricimonas faecihominis
JCM 18676
T
, generated by nanopore sequencing. The genome consists of a single circular chromosome of 4,851,806 bp, with a G + C content of 42.9%, and was predicted to contain 15 rRNA and 61 tRNA genes and encode for 3,946 proteins.
Zinc excess impairs
Mycobacterium bovis
growth through triggering a Zur-IdeR-iron homeostasis signal pathway
Authors: Li Xiaohui , Chen Liu , Wang Yuankun , Guo Xiao , He Zheng-Guo 
Published: September 2023

ABSTRACT

Zinc excess is toxic to bacteria and, thus, represents an important innate defense mechanism of host cells, especially against mycobacterial infections. However, the signaling pathway triggered by zinc excess and its relationship with iron homeostasis remain poorly understood in mycobacteria. Here, we characterize a novel Zur-IdeR-iron homeostasis signaling pathway that modulates the growth of
Mycobacterium bovis
under zinc toxicity. We found that the regulator Zur interacts with the iron-homeostasis regulator IdeR, enhancing the DNA-binding ability of IdeR. Excess zinc disrupts this interaction and represses
ideR
transcription through Zur, which promotes the expression of iron uptake genes and leads to the accumulation of intracellular iron in
M. bovis
. The elevated iron levels lower the bacterial survival ability under excess zinc stress. Consistently, deleting
zur
hinders intracellular iron accumulation of
M. bovis
and enhances bacterial growth under stress, while silencing
ideR
impairs the growth of the wild-type and
zur
-deleted strains under the same conditions. Interestingly, both Zur and IdeR are conserved in bacteria facing zinc toxicity. Overall, our work uncovers a novel antimicrobial signal pathway whereby zinc excess disrupts iron homeostasis, which may deepen our understanding of the crosstalk mechanism between iron and zinc homeostasis in bacteria.

IMPORTANCE

As a catalytic and structural cofactor of proteins, zinc is essential for almost all living organisms. However, zinc excess is toxic and represents a vital innate immunity strategy of macrophages to combat intracellular pathogens, especially against mycobacterial pathogens such as
Mycobacterium tuberculosis
, the causative agent of tuberculosis. Here, we first characterize an antibacterial signaling pathway of zinc excess and its relationship with iron homeostasis in
M. bovis
. We found that excess zinc inhibits the transcription of
ideR
and its DNA-binding activity through Zur, which, in turn, promotes the expression of iron uptake genes, causes intracellular iron accumulation, and finally impairs the bacterial growth. This study reveals the existence of the Zur-IdeR-iron homeostasis pathway triggered by zinc excess in
M. bovis
, which will shed light on the crosstalk mechanisms between zinc and iron homeostasis in bacteria and the antimicrobial mechanisms of host-mediated zinc toxicity.
Listeria monocytogenes
GshF contributes to oxidative stress tolerance via regulation of the phosphoenolpyruvate-carbohydrate phosphotransferase system
Authors: Chen Mianmian , Zhang Jiaxue , Xia Jing , Sun Jing , Zhang Xian , Xu Jiali , Deng Simin , Han Yue , Jiang Lingli , Song Houhui , Cheng Changyong 
Published: September 2023

ABSTRACT

Glutathione (GSH) is an essential component of the glutaredoxin (Grx) system, and it is synthesized by the enzyme glutathione synthase GshF in
Listeria monocytogenes
. GSH plays a crucial role in regulating
Listeria
virulence by modifying the virulence factors LLO and PrfA. In this study, we investigated the involvement of
L. monocytogenes
GshF in oxidative tolerance and intracellular infection. Our findings revealed that the deletion of
gshF
resulted in a significant reduction in bacterial growth
in vitro
when exposed to diamide and copper ions stress. More importantly, this deletion also impaired the efficiency of invasion and proliferation in macrophages and mice organs. Furthermore, GshF influenced global transcriptional profiles, including carbohydrate and amino acid metabolism, particularly those related to the phosphoenolpyruvate-carbohydrate phosphotransferase system (PTS) genes
lmo1997-lmo2004
, under oxidative stress conditions. In the wild-type strain, the transcription of
lmo1997-lmo2004
was notably downregulated in response to copper ions and diamide stress compared to normal conditions. However, in the absence of
gshF
, the transcripts of
lmo1997-lmo2004
were upregulated in response to these stress conditions. Notably, the deletion of

iiB
man

(
lmo2002
) enhanced oxidative stress tolerance to copper ions, whereas overexpression of

iiB
man

reduced this resistance. In conclusion, our study provides the first evidence that
L. monocytogenes
GshF plays a crucial role in bacterial antioxidation through the regulation of

iiB
man

.

IMPORTANCE

Listeria monocytogenes
has developed various mechanisms to withstand oxidative stress, including the thioredoxin and glutaredoxin systems. However, the specific role of the glutathione synthase GshF, responsible for synthesizing GSH in
L. monocytogenes
, in oxidative tolerance remains unclear. This study aimed to elucidate the relationship between GshF and oxidative tolerance in
L. monocytogenes
by examining the efficiency of invasion and proliferation in macrophages and mice organs, as well as analyzing global transcriptional profiles under oxidative stress conditions. The results revealed that GshF plays a significant role in
L. monocytogenes
’ response to oxidative stress. Notably, GshF acts to suppress the transcription of phosphoenolpyruvate-carbohydrate phosphotransferase system genes
lmo1997-lmo2004
, among which

iiB
man

(
lmo2002
) was identified as the most critical gene for resisting oxidative stress. These findings enhance our understanding of how
L. monocytogenes
adapts to its environment and provide valuable insights for investigating the environmental adaptation mechanisms of other pathogenic bacteria.
Evolution and distribution of rabies viruses from a panorama view
Authors: Li Gen , Zhang Yue , He Hong-Ling , Chen Chang-Yi , Li Xin , Xiao Yu , Yan Zhi-Bin , Chu Ying , Luo Jun , Guo Xiao-Feng 
Published: September 2023

ABSTRACT

Rabies kills more than 59,000 people annually, mainly in developing countries. Previous studies on the evolution and distribution of rabies viruses (RABVs) were scattered. Here, we explore the evolution and distribution of this deadly virus from a novel panorama view. Multiple bioinformatic software tools were employed to analyze the phylogenetic diversity, evolution, spatiotemporal, and distribution of RABVs. The analyses were based on 1,202 qualified full-length genomes of RABVs and numerous literatures. Of the 10 distinct phylogenetic clades of RABV that we identified, more frequent intra- and inter-clade recombination occurs in the sequences of Asian-SEA, Arctic, and Cosmopolitan clades isolated from China, while according to existing sequence information, RABV might originate from bats (posterior probability, PP = 0.75, PP = 0.60 inferred from N and L genes, separately) in North America (PP = 0.57, PP = 0.62 inferred from N and L genes, separately). Due to the difference in evolutionary rate of N (2.22 × 10
−4
subs/site/year, 95% HPD 1.99–2.47 × 10
−4
subs/site/year) and L genes (1.67 × 10
−4
subs/site/year, 95% HPD 1.59–1.74 × 10
−4
subs/site/year), the root age was 1,406.6 (95% HPD 1,291.2–1,518.2) and 1,122.7 (95% HPD 1,052.4–1,193.9) inferred from N and L genes, separately. Among other findings, Mephitidae plays an important role in the interspecific transmission and communication of RABV, which we found tends to spread to populations genetically proximate to the host. We also identified amino acids under positive selection in different genes of different clades as well as single nucleotide variation sites important for different lineages.

IMPORTANCE
Rabies virus is widely distributed all over the world, and wild animals are its largest potential reservoir. Our study offers a panorama view about evolution and distribution of rabies viruses and emphasizes the need to monitor the transmission dynamics of animal rabies.
Sacral stress fracture in a young-adult, long-distance runner: an underestimated cause of low back pain
Authors: Pinheiro Joana Páscoa , Rovisco Branquinho Lurdes , Pinheiro João 
Published: September 2023

Sacral stress fractures (SSFs) in physically active young patients are frequently misdiagnosed due to the lack of specificity of signs and symptoms. Over the last years, these injuries have been described as rare, although some studies report that the incidence of an SSF in athletes may be as high as 20%.We describe a case of a male long-distance runner in his late 20s with a 1-month undiagnosed SSF. The patient complaints included insidious right low back and buttock pain without trauma that started after running a marathon. MRI revealed an extensive area of bone marrow oedema in the right sacral ala consistent with an SSF.This case highlights the importance of investigating SSF in young-athlete patients who were otherwise healthy, using appropriate imaging modalities to assess the presence and morphology of a fracture.
Fine visuomotor skills in amblyopia: a systematic review and meta-analysis
Authors: Rakshit Archayeeta , Schmid Katrina L , Webber Ann L 
Published: September 2023

BackgroundAmblyopia is characterised by reduced visual acuity, poor binocular sensory fusion, and impaired or absent stereoacuity. Understanding the extent to which amblyopia affects everyday task performance is important to quantifying the disease burden of amblyopia and can assist clinicians to understand patients’ likely functional capability.MethodsA systematic literature search identified published studies comparing fine visuomotor performance in either children or adults with amblyopia and those with normal binocular vision. The included studies (22 studies involving 835 amblyopes and 561 controls) reported results of self-perception patient reported outcome measures, tests of motor proficiency and video recorded reaching and grasping. The outcomes of 17 studies were grouped into four meta-analyses, with pooled results reported as standardised mean difference (SMD) with corresponding 95% CI.ResultsRegardless of the cause of amblyopia (anisometropia, strabismus, mixed, deprivation), significant reduction in self-perception of physical competence and athletic competence (SMD=−0.74, 95% CI −1.23 to −0.25, p=0.003); fine motor skills scores (SMD=−0.86, 95% CI −1.27 to −0.45, p<0.0001); speed of visually guided reaching and grasping movements (SMD=0.86, 95% CI 0.65 to 1.08, p<0.00001); and precision of temporal eye-hand coordination (SMD=0.75, 95% CI 0.26 to 1.25, p=0.003) occurred in amblyopes compared with those with normal visual development.ConclusionReports of the impact of amblyopia on fine motor skills performance find poorer outcomes in participants with amblyopia compared with those with normal vision development. Consistency in the outcome measure used to assess the functional impact of amblyopia would be valuable for future studies.