bluuebunny/crossref_metadata_2025_split huggingface dataset:
Risk factors for low oocyte retrieval in patients with polycystic ovarian syndrome undergoing in vitro fertilization
Authors: Jin Hao , Yang Haiyan , Zheng Jiujia , Zhou Jiechun , Yu Rong 
Published: July 2023

Abstract
Background
The number of oocytes retrieved does not always coincide with the number of follicles aspirated in in vitro fertilization/intracytoplasmic sperm injection (IVF/ICSI) treatment. Patients with high expectation of retrieval sometimes obtain few oocytes, which may be induced by improper operation or therapeutic factors. The purpose of this study was to evaluate the distribution data of oocyte retrieval rate (ORR) and to explore the risk factors for low ORR in patients with polycystic ovary syndrome (PCOS) undergoing IVF/ICSI.

Methods
A total of 2478 patients with PCOS undergoing IVF/ICSI were involved in this retrospective case-control study from March 2016 to October 2021. The oocyte retrieval rate was calculated as the ratio of the number of obtained oocytes to the number of follicles (≥ 12 mm) on the trigger day. Patients were divided into a low ORR and a normal ORR group with the boundary of one standard deviation from the mean value of ORR. The patient characteristics, treatment protocols, serum hormone levels, and embryonic and pregnancy outcomes were analyzed.

Results
The ORR exhibited a non-normal distribution, with a median of 0.818. The incidence of complete empty follicle syndrome was 0.12% (3/2478). The proportion of patients in the low ORR group who received the progestin-primed protocol was significantly higher than that in the normal ORR group (30.30% vs. 17.69%). A logistic regression analysis showed that the serum estradiol level/follicle (≥ 12 mm) ratio (OR: 0.600 (0.545–0.661)) and progesterone level (OR: 0.783 (0.720–0.853)) on the trigger day were significant factors in the development of a low ORR, with optimal cutoff values of 172.85 pg/ml and 0.83 ng/ml, respectively, as determined by receiver operating curve. Fewer high-quality embryos (2 vs. 5) and more cycles with no available embryos (5.42% vs. 0.43%) were found in the low ORR group.

Conclusions
For patients with PCOS, low estradiol levels/follicles (≥ 12 mm) and progesterone levels on the trigger day and the use of the progestin-primed protocol could be risk factors for low ORR, which leads to a limited number of embryos and more cycle cancellations.
Adherence to the Paleolithic diet and Paleolithic-like lifestyle reduce the risk of colorectal cancer in the United States: a prospective cohort study
Authors: Xiao Yi , Wang Yaxu , Gu Haitao , Xu Zhiquan , Tang Yunhao , He Hongmei , Peng Linglong , Xiang Ling 
Published: July 2023

AbstractBackgroundThe plant-based paleolithic diet (PD) and the paleolithic-like lifestyle (PLL) may reduce the risk of chronic diseases, including colorectal adenomas. These dietary and lifestyle approaches are proposed to exert their effects through mechanisms such as reducing inflammation, oxidative stress, and insulin levels. However, whether PD and PLL is associated with the risk of colorectal cancer (CRC) has not been determined.MethodsA cohort of 74,721 individuals who participated in the PLCO study were included in this analysis. Adherence to the PD and PLL was assessed using PD and PLL scores, where higher scores indicated greater adherence. Multivariable Cox models were utilized to estimate hazard ratios (HRs) and 95% confidence intervals (CIs) for the risk of CRC and its subsites (proximal colon cancer and distal CRC). Subgroup analyses were conducted to identify potential effect modifiers.ResultsDuring a mean follow-up of 9.2 years, a total of 694 CRC cases were identified. Participants in the highest compared with the lowest quartiles of PD score had a lower risk of CRC (Q4 vs Q1: HR 0.76, 95% CI 0.61–0.95,Ptrend = 0.009) and proximal colon cancer (Q4 vs Q1: HR 0.73, 95% CI 0.55–0.97,Ptrend = 0.02). A stronger inverse association was observed for PLL score with the risk of CRC (Q4 vs Q1: HR 0.64, 95% CI 0.51–0.81,Ptrend < 0.001), proximal colon (Q4 vs Q1: HR 0.62, 95% CI 0.46–0.83,Ptrend = 0.001) and distal CRC (Q4 vs Q1: HR 0.69, 95% CI 0.48–0.98,Ptrend = 0.03). Subgroup analyses revealed the inverse association of PD score with the risk of CRC was more pronounced in participants with BMI < 30 (Q4 vs Q1: HR 0.68, 95% CI 0.53–0.87) than in those with BMI ≥ 30 (Q4 vs Q1: HR 1.07, 95% CI 0.68–1.67) (Pinteraction = 0.02).ConclusionsOur findings suggest that adhering to the PD and PLL could be a new option to reduce CRC risk.
Effectiveness of school-based sexual and reproductive health education among adolescent girls in Urban areas of Odisha, India: a cluster randomized trial
Authors: Alekhya G. , Parida Swayam Pragyan , Giri Prajna Paramita , Begum Jasmina , Patra Suravi , Sahu Dinesh Prasad 
Published: July 2023

Abstract
Background
Various studies revealed that adolescent girls have limited knowledge pertaining to sexual and reproductive health (SRH). The current study assessed the effectiveness of SRH education among adolescent girls in urban areas of Odisha, India.

Methods
The study design was a cluster randomized trial, where the clusters composed of eight Odia (regional language) medium government girls’ high schools in Bhubaneswar, the capital city of the state of Odisha, India. For the selection of study participants, adolescent girls who were studying in the ninth and tenth standards were recruited from each school. Eight schools were randomized through restrictive randomization at a 1:1 ratio, with four schools each in the intervention and control arm. Baseline and end-line assessments were done using a pre-tested, semi-structured questionnaire. Following baseline assessment, an intervention was given with the help of handbooks developed by the study authors to the schools in the intervention arm. Outcomes included change in knowledge, attitude and practices pertaining to SRH.

Results
In our study at baseline, there were a total of 790 students, where 469 (59.4%) students were in the intervention arm, and 321 (40.6%) students were in the control arm. At baseline, only 282 (60.1%) in the intervention arm and 171 (53.3%) in the control arm were aware that physical bodily changes due to puberty were normal. After the intervention, there was a statistically significant increase in knowledge in intervention group 367 (94.8%) (p-value < 0.001). Most students used sanitary pads as absorbent, 97.2% in the intervention group and 98.4% in the control group. However, after the intervention, the use of other absorbents reduced to zero in the intervention group with a statistically significant difference (p < 0.05). The number of students having awareness on different methods of contraception increased from 51 (10.9%) to 337 (87.1%) in the intervention arm (p < 0.001), and of those having awareness on STIs/RTIs increased from 177 (38.2%) to 371 (96.1%) in the intervention group (p < 0.001).

Conclusion
From our study, there is a significant proportional change in knowledge, attitude, and practices pertaining to SRH. Our study recommends policymakers and program managers for the implementation of comprehensive SRH in the regular school curriculum.
Trial registration CTRI/2021/01/030490, registered on January 15, 2021. Prospectively registered at https://ctri.nic.in/Clinicaltrials/login.php
Cervical carcinogenesis risk association of HPV33 E6 and E7 genetic variations in Taizhou, Southeast China
Authors: Yan Zi-Yi , Di Xing-Hong , Qiu Yi , Ying Yuan-Yuan , Gan Jun , Xu Hui-Hui 
Published: July 2023

Abstract
Background
Human papillomavirus (HPV) 33 belongs to the Alphapapillomavirus 9 (α-9 HPV) species group, which also contains types 16, 31, 35, 52, 58 and 67. The purpose of this study was to investigate the genetic variations of HPV33 and to explore its carcinogenicity among women in Taizhou, Southeast China.

Methods
Exfoliated cervical cells were collected for HPV genotyping. Only single HPV33 infection cases were selected, and their E6 and E7 genes were sequenced using the ABI 3730xl sequencer and then analysed using MEGA X.

Results
From 2014 to 2020, a total of 185 single HPV33-positive specimens were successfully amplified. We obtained 15 distinct HPV33 E6/E7 variants, which were published in GenBank under accession numbers OQ672665-OQ672679. Phylogenetic analysis revealed that all HPV33 E6/E7 variants belonged to lineage A, of which 75.7% belonged to lineage A1. Compared with CIN1, the proportion of sublineage A1 in CIN2/3 was higher, but there was no significant difference (76.5% vs. 80.6%, P > 0.05). Altogether, 20 single nucleotide substitutions were identified, of which 6 were novel substitutions, including T196G (C30G), A447T, G458T (R117L), G531A, A704A, and C740T. In addition, no significant trends were observed between the nucleotide substitutions of HPV33 E6/E7 variants and the risk of cervical lesions.

Conclusion
This study provides the most comprehensive data on genetic variations, phylogenetics and carcinogenicity of HPV33 E6/E7 variants in Southeast China to date. The data confirmed that cervical lesions among women in Taizhou are attributable to HPV33, which may be due to the high infection rate of sublineage A1 in the population.
Termite diversity and abundance based on altitude in Mount Ungaran, Central Java, Indonesia
Authors: SUBEKTI NIKEN , MILANIO RODERIKUS RAYDITYA 
Published: July 2023

Abstract. Subekti N, Milanio RR. 2023. Termite diversity and abundance based on altitude in Mount Ungaran, Central Java, Indonesia. Biodiversitas 24: 3332-3337. Termites are social insects whose main diet is the cellulose and lignocellulose found in rotting wood and in some fungi. As the Mount Ungaran area in Central Java, Indonesia, has been converted to agriculture and other purposes, termite habitat has become increasingly narrow, with a reduction in the insects’ food sources. The purpose of this study was to analyze the species of termites found on Mount Ungaran and the factors that influence their lives. In addition, the differences in species according to slope (north, south, east, and west) and altitude were assessed. This research was conducted in three stages: termite collection, termite identification, and data analysis. The main species found in this study were Coptotermes curvignathus Holmgren, 1913, Odontotermes javanicus Holmgren, 1912, Macrotermes gilvus Hagen, 1858, Nasutitermes matangensis Haviland, 1898, and Bulbitermes constrictiformis Holmgren, 1914. Based on a Shannon-Wiener analysis, the west slope had the highest diversity index, which was primarily influenced by four environmental factors (soil pH, soil temperature, soil moisture, and rainfall). In the canonical relationship correspondence analysis, soil pH, light intensity, and canopy cover were the environmental factors that most affected the diversity and abundance of termites on Mount Ungaran. The conclusions that can be drawn from this study are that termite richness varied between slopes and the environmental factors that were the most associated with the variation were soil pH, light intensity, and canopy cover.
Bird diversity and mangrove forest as potential ecotourism destinations in Kapo-kapo Bay, Cubadak Island, West Sumatra, Indonesia
Authors: NOVARINO WILSON , MUKHTAR ERIZAL , PUTRI AYU SMARNIA , ANGGRAINI PUTRI LISYA 
Published: July 2023

Abstract. Novarino W, Mukhtar E, Putri AS, Anggraini PL. 2023. Bird diversity and mangrove forest as potential ecotourism destinations in Kapo-kapo Bay, Cubadak Island, West Sumatra, Indonesia. Biodiversitas 24: 3583-3591. Mangrove forests play an important role in the management of the coastal ecosystem of Indonesia. Research on bird diversity has been carried out to support ecotourism attractions in the mangrove area of Kapo-kapo Bay, West Sumatra. The objective of this study is to determine the bird diversity and mangrove plant diversity in the mangrove kapo-kapo Bay area, West Sumatra, using the point count method and transect method. Mangrove plants have been found in three families, five genera, and six species. The vegetation composition on this research site is lower than that on Sumatra's east coast. The composition of mangrove trees and tree diversity was lower on the west coast of Sumatra than on the east coast. The composition and diversity of birds at the study sites were not significantly different between the mangrove forests on Sumatra's west and east coasts. Rhizophora apiculata Blume species dominate the mangrove forests in Kapo-kapo Bay, while Collocalia esculenta species dominate the birds. The analysis of the suitability of mangrove ecotourism in Kapo-kapo Bay obtained a score of 111 or 92.5%, placing it in the Very Suitable category (S1) to be developed as an ecotourism area. The presence of birds and bird habitat use in mangroves are all interesting ecotourism attractions.
Isolation, characterization and identification of sponge-associated bacteria producing antimicrobial compounds
Authors: WIBOWO RISKY HADI , SIPRIYADI SIPRIYADI , DARWIS WELLY , PUTRI DELFIANI ANGGIAS , YUDHA SALPRIMA , MASHUDI MASHUDI , ILSAN NOOR ANDRYAN , RENTA PERSON PESONA 
Published: July 2023

Abstract. Wibowo RH, Sipriyadi, Darwis W, Putri DA, Yudha S, Mashudi, Ilsan NA, Renta PP, Masrukhin. 2023. Isolation, characterization and identification of sponge-associated bacteria producing antimicrobial compounds. Biodiversitas 24: 3616-3623. Sponges are marine biota that is currently in great demand as research object. Sponges also belong to a group of marine life that has symbiotic with bacteria. Bacteria that have formed a symbiotic relationship with a sponge may produce secondary metabolites that protect their host from pathogens. This study aimed to isolate, select, characterize and identify bacteria associated with the sponge Aplysina sp. that exhibit antimicrobial activity. The methods used in this study were bacterial isolation, screening, molecular identification, and observing their ability to produce antimicrobial compounds. A total of 16 isolates were isolated on the Sea Water Complete agar medium, and four isolates were able to inhibit the growth of pathogenic microbes including Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, and Candida albicans. Four isolates with the most significant inhibitory clear zones were selected for further testing. The isolate APD10 showed the biggest inhibition zone. The molecular identification results showed that the APD3 and APD15 had genetic similarities with Bacillus subtilis, isolate APD9 had genetic similarities with Bacillus paralicheniformis, and isolate APD10 had genetic similarities with Bacillus velezensis. Two isolates (APD3 and APD15) gene sequences for the KS domain in PKS were related to polyketide synthase, while APD10 in KS was related to oxidoreductase. Two isolates (APD3 and APD15) gene sequences encoding domain A on NRPS had a relationship with a bioactive compound in the form of surfactin, APD9 was related to a bioactive compound in the form of bacitracin, and APD10 NRPS was related to adenylation.
Promoting insect farming and household consumption through agricultural training and nutrition education in Africa: A study protocol for a multisite cluster-randomized controlled trial
Authors: Alemu Mohammed Hussen , Halloran Afton , Olsen Søren Bøye , Anankware Jacob Paarechuga , Nyeko Philip , Ayieko Monica , Nyakeri Evans , Kinyuru John , Konyole Silvenus , Niassy Saliou , Egonyu James Peter , Malinga Geoffrey Maxwell , Ng’ang’a Jeremiah , Ng’ong’a Charles Adino , Okeyo Nicky , Debrah Shadrack Kwaku , Kiiru Samuel , Acur Amos , Roos Nanna 
Published: July 2023

Background
Edible insects are a sustainable source of high-quality animal protein. Insect farming is gaining interest globally, particularly in low-income countries, where it may provide substantial nutritional and economic benefits. To enhance insect farming practices in Africa, new farming systems are being developed. However, knowledge on how to best promote uptake of these systems is lacking. This study aims to fill this gap by investigating the effectiveness of educational interventions in promoting insect farming for household consumption in Africa.

Method
The study is designed as a multi-site randomized controlled trial to evaluate the impacts of agricultural training alone or in combination with nutrition education on the adoption of insect farming in Ghana, Kenya and Uganda. In each of the three countries, ninety-nine villages are randomly assigned to one of three arms: two intervention arms and a control arm with no interventions. Focusing on production (P), the first intervention arm covers agricultural training on insect farming combined with provision of insect production starter kits. Focusing on both production and consumption (PC), the second intervention arm involves the same intervention components as treatment P plus additional nutrition education. The impacts of the interventions are measured by comparing baseline and endline data collected one year apart. Primary outcomes are adoption of insect farming and consumption of the farmed insects.

Discussion
Understanding the drivers and impacts of novel agricultural practices is crucial for transitioning to sustainable food systems. The current project is the first to investigate how educational interventions promote insect farming for household consumption in low-income countries. The results will contribute evidence-based knowledge to support sustainable development through insect farming in Africa.

Trial registration
The protocol is registered in the American Economic Association registry for randomized control trials with registration number AEARCTR-0009996. Initial registration date: 02 September 2022, last updated 17 May 2023.
Soft linguistic terrorism: 21st century re-articulations
Authors: Mena Mike 
Published: July 2023

Abstract
Over three decades ago, Gloria Anzaldúa identified ideologies of linguistic standardization as an oppressive force in the Rio Grande Valley of south Texas as well as the local university. Such ideologies were used to delegitimize Chicanos via “linguistic terrorism,” or, routine forms of psychological and physical punishment meant to enforce idealized white, middle-class, monolingual social norms. However, times have changed. To account for more recent conditions, I qualify contemporary manifestations as soft linguistic terrorism, which relies more so on incentivization (reward as opposed to punishment) and ideological recruitment (enforcement based on the appearance of consent), yet continue to reproduce the near identical racializing ideologies Anzaldúa identified decades ago. Using a linguistic anthropological approach to discourse analysis, this article focuses on ethnographic interviews with students and faculty to illustrate how forms of linguistic terrorism have been rearticulated via raciolinguistic ideologies in the same region and at the same university that inspired Anzaldúa’s formulation of linguistic terrorism in the 1980s.
Hutang Piutang dalam Tradisi Bhen Gibhen pada acara Pernikahan di Madura: Studi Living Qur’an Desa Daleman Galis Bangkalan.
Authors: Sama'un Sama'un , Muin Abd 
Published: July 2023

Madura adalah satu dari jajaran kepualauan tropis yang beriklim panas dan bertanah tandus. Faktor inilah yang membuat karakter orang Madura keras dan temprament. Namun demikian, Madura yang sebagian besar penduduknya memeluk agama Islam, pasti ada akulturasi nila-nilai keislaman yang turut mewarnai situasi sosial dan budaya masyarakat Madura. Melalui penelitian lapangan dan pustaka, dengan pendekatan diskriptif analisis dan metode living quran pada masyarakat Daleman kecamatan Galis, kabupaten Bangkalan yang berfokus pada tradisi bhen ghiben sebagai objek formalnya. Penulis menemukan, bahwa tradisi bhen ghiben dalam budaya bey ghebey di acara pernikahan di Daleman, Galis kab Bangkalan, dianggap sebagai hutang yang dibebankan kepada tuan rumah oleh tamu undangan dan hutang piutang tersebut dicatat oleh pihak ketiga untuk meminimalisir kesalah-pahaman antara pemilik hutang dan yang mempunyai piutang. Sistem pencatatan hutang piutang merupakan implementasi dari ajaran al-Quran, tepatnya dalam surat al-Baqarah ayat 238. Oleh karena implementasi nilai al-Quran tersebut berakulturasi membentuk sebuah budaya yang telah mengakar di desa Daleman, maka wujud pencatatan hutang piutang dalam tradisi bhen ghiben dapat dikategorikan sebagai quranisasi sebuah kehidupan yang telah membudaya sebagai hasil respon dari sisi eksegis.masyarakat Daleman terhadap al-Quran.
Surgical Site Infection in Patients Managed with an Endoprosthesis for the Treatment of Cancer
Authors: Ray G.S. , Werth P. , Alexander J.H. , Eward W.C. , Bernthal N.M. , Jeys L.M. , Funovics P. , Windhager R. , Temple H.T. , Lozano-Calderon S. , Avedian R.S. , Jutte P.C. , Ghert M. , Ruggieri P. , Henderson E.R. ,   on behalf of the PARITY Investigators
Published: July 2023

Background:
Surgical site infection (SSI) after segmental endoprosthetic reconstruction in patients treated for oncologic conditions remains both a devastating and a common complication. The goal of the present study was to identify variables associated with the success or failure of treatment of early SSI following the treatment of a primary bone tumor with use of a segmental endoprosthesis.

Methods:
The present study used the Prophylactic Antibiotic Regimens in Tumor Surgery (PARITY) data set to identify patients who had been diagnosed with an SSI after undergoing endoprosthetic reconstruction of a lower extremity primary bone tumor. The primary outcome of interest in the present study was a dichotomous variable: the success or failure of infection treatment. We defined failure as the inability to eradicate the infection, which we considered as an outcome of amputation or limb retention with chronic antibiotic suppression (>90 days or ongoing therapy at the conclusion of the study). Multivariable models were created with covariates of interest for each of the following: surgery characteristics, cancer treatment-related characteristics, and tumor characteristics. Multivariable testing included variables selected on the basis of known associations with infection or results of the univariable tests.

Results:
Of the 96 patients who were diagnosed with an SSI, 27 (28%) had successful eradication of the infection and 69 had treatment failure. Baseline and index procedure variables showing significant association with SSI treatment outcome were moderate/large amounts of fascial excision ≥1 cm2) (OR, 10.21 [95% CI, 2.65 to 46.21]; p = 0.001), use of local muscle/skin graft (OR,11.88 [95% CI, 1.83 to 245.83]; p = 0.031), and use of a deep Hemovac (OR, 0.24 [95% CI, 0.05 to 0.85]; p = 0.041). In the final multivariable model, excision of fascia during primary tumor resection was the only variable with a significant association with treatment outcome (OR, 10.21 [95% CI, 2.65 to 46.21]; p = 0.018).

Conclusions:
The results of this secondary analysis of the PARITY trial data provide further insight into the patient-, disease-, and treatment-specific associations with SSI treatment outcomes, which may help to inform decision-making and management of SSI in patients who have undergone segmental bone reconstruction of the femur or tibia for oncologic indications.

Level of Evidence:
Prognostic Level III. See Instructions for Authors for a complete description of levels of evidence.