bluuebunny/crossref_metadata_2025_split huggingface dataset:
PENGUATAN KAPASITAS UMKM TERHADAP PERIZINAN BERUSAHA, SERTIFIKAT HALAL DAN DIGITALISASI PEMASARAN DI KELURAHAN BUGIS KECAMATAN DUMBO RAYA KOTA GORONTALO
Authors: Imran Mohammad , Farid  , Putalan Reinal 
Published: January 2025

Tantangan yang dihadapi UMKM di Kelurahan Bugis Kecamatan Dumbo Raya Kota Gorontalo, diantaranya keterbatasan pengetahuan tentang perizinan usaha, rendahnya tingkat kepatuhan terhadap sertifikasi halal dan minimnya pemanfaatan teknologi digital untuk pemasaran. Oleh karenanya, kegiatan ini bertujuan untuk memberikan pemahaman dan penguatan kepada Pelaku UMKM yang ada di Kelurahan Bugis Kecamatan Dumbo Raya Kota Gorontalo dengan metode: (1) Bimbingan Teknis Pembuatan NIB; (2) Pelatihan dan pendampingan pembuatan akun siHalal dan (3) Sosialisasi terkait digitalisasi pemasaran produk UMKM. Pelaksanaan kegiatan meliputi sosialisasi terkait digitalisasi pemasaran produk UMKM, dilanjutkan dengan pemaparan materi, pelatihan dan bimbingan teknis yang dilaksanakan secara langsung terkait Pembuatan NIB dan pembuatan akun siHalal. Output kegiatan ini, menumbuhkan kesadaran pelaku usaha UMKM agar memiliki legalitas usaha dengan Nomor Induk Berusaha (NIB) dan sebagai penguatan kapasitas para pelaku usaha UMKM terkait sertifikasi Halal dan Pemasaran produk secara digital.
Strategies for using sustainable urban drainage systems in the architectural design of civil infrastructure projects in Jakarta
Authors: Gbran Hassan 
Published: January 2025

The current research is mainly targeting the major challenge of climate change and flood risk in Indonesia (Jakarta) through investigations on suitable methods of sustainable urban drainage systems (SUDS). Through climate scenarios and flood risk assessments, as well as SUDS efficiency appraisals, this study aims at helping stakeholders gain insight on innovative water management strategies. The combination of hydrological models, GIS analysis, and stakeholdersâ€™ engagement is the research approach that will enable the exploration of Jakarta's vulnerability to extreme weather events and introduce mitigation techniques in the form of SUDS. This study aims to address climate changeâ€™s impact on flood risk and develop sustainable design solutions so that the infrastructure in Jakarta can be resilient to flooding. The findings of this research are to provide a basis for decisions, plans, and policies towards green water management regulations and a more water-resilient urban built environment.
Study of Method of Evaluating Surface's Pollution Level & Cleaning Efficiency on Decoration Building Materials
Authors: O Un Gyong , Kim Kyong Chon , Jo Jong Chol 
Published: January 2025

In the paper, we analyzed synthetically the research results on methods of testing the cleaning effect on DBM(Decoration Building Materials) and suggested a new Pollution Level & Cleaning Effect Evaluation System(PCEES) on the DBM's surface which is based on image information processing by use of color quantization method and color feature extraction method with HSI (Hue Saturation Intensity) model.
By applying the PCEES to many building objects, we could select the effective composition of cleaning agent and cleaning method and calculate the agent's consumption amount necessary for the building work.
And we suggested the degree of cleaning effect according to the quantitative result values of evaluation on pollution level & cleaning effect to correspond with the empirical judgment of a man.
Efektivitas Senam Mata Kombinasi Relaksasi terhadap Kelelahan Mata pada Siswa SMP Negeri 3 Kabupaten Sorong
Authors: Atanay Yulia Ria Mariyana , Situmorang Lourdes 
Published: January 2025

ABSTRACT Eyestrain is an eye health problem that is increasingly common among school children. Excessive use of gadgets causes increased eye health problems in school children. One effort to reduce eye fatigue is by doing eye exercises combined with relaxation. To determine the effectiveness of eye exercises combined with relaxation on eye fatigue in students at SMPN 3 Sorong Regency. Quantitative research, quasi-experimental research design with a pre-post test design approach without a control group. The total sample was 50 students at SMPN 3 Sorong Regency who experienced eye fatigue. The sampling technique uses the Simple Random Sampling technique. The research instrument used a questionnaire. Data analysis used the Wilcoxon test. The Wilcoxon Test statistical test obtained a P-value of 0.000 < 0.05, meaning that there was a difference between the eye fatigue values before and after being given a combination of relaxation eye exercises. Providing a combination of eye relaxation techniques is effective in reducing eye fatigue in students at SMP Negeri 3 Sorong Regency. Suggestions for health workers and schools to educate children at school for always apply eye exercises combined with relaxation to reduce symptoms of eye fatigue due to the use of gadgets. Keywords: Eye Exercises, Relaxation, Eye Fatigue, Gadget Use ABSTRAK Kelelahan mata adalah masalah kesehatan pada mata yang semakin umum terjadi di kalangan anak sekolah. Penggunaan gadget secara berlebihan ditengarai menjadi penyebab meningkatnya problem masalah kesehatan mata pada anak sekolah. Salah satu upaya untuk mengurangi kelelahan mata yaitu dengan melakukan senam mata kombinasi relaksasi. Untuk mengetahui efektivitas senam mata kombinasi relaksasi terhadap kelelahan mata pada siswa SMPN 3 Kabupaten Sorong. Penelitian kuantitatif, desain penelitian quasy experiment dengan pendekatan pre-post test design without control group. Jumlah sampel berjumlah 50 Siswa-siswi SMPN 3 Kabupaten Sorong yang mengalami kelelahan mata. Teknik pengambilan sampel menggunakan teknik Simple Random Sampling. Instrument penelitian menggunakan kuesioner. Analisis data menggunakan uji Wilcoxon test. Uji statistik Wilcoxon Test diperoleh P-value sebesar 0,000 < 0,05, artinya terdapat perbedaan antara nilai kelelahan mata sebelum dan sesudah diberikan senam mata kombinasi relaksasi. Pemberian senam mata kombinasi relaksasi efektif terhadap penurunan Kelelahan Mata Pada Siswa SMP Negeri 3 Kabupaten Sorong. Saran bagi tenaga kesehatan dan pihak sekolah dapat mengedukasi anak-anak di sekolah agar selalu menerapkan senam mata kombinasi relaksasi untuk mengurangi gejala kelelahan mata akibat penggunaan gadget. Kata Kunci: Senam Mata, Relaksasi, Kelelahan Mata, Penggunaan Gadget
Analysis and validation of serum biomarkers in brucellosis patients through proteomics and bioinformatics
Authors: Li Xiao , Wang Bo , Li Xiaocong , He Juan , Shi Yue , Wang Rui , Li Dongwei , Haitao Ding 
Published: January 2025

IntroductionThis study aims to utilize proteomics, bioinformatics, and machine learning algorithms to identify diagnostic biomarkers in the serum of patients with acute and chronic brucellosisMethodsProteomic analysis was conducted on serum samples from patients with acute and chronic brucellosis, as well as from healthy controls. Differential expression analysis was performed to identify proteins with altered expression, while Weighted Gene Co-expression Network Analysis (WGCNA) was applied to detect co-expression modules associated with clinical features of brucellosis. Machine learning algorithms were subsequently used to identify the optimal combination of diagnostic biomarkers. Finally, ELISA was employed to validate the identified proteins.ResultsA total of 1,494 differentially expressed proteins were identified, revealing two co-expression modules significantly associated with the clinical characteristics of brucellosis. The Gaussian Mixture Model (GMM) algorithm identified six proteins that were concurrently present in both the differentially expressed and co-expression modules, demonstrating promising diagnostic potential. After ELISA validation, five proteins were ultimately selected.DiscussionThese five proteins are implicated in the innate immune processes of brucellosis, potentially associated with its pathogenic mechanisms and chronicity. Furthermore, we highlighted their potential as diagnostic biomarkers for brucellosis. This study further enhances our understanding of brucellosis at the protein level, paving the way for future research endeavors.
Prevalence and determinants of ideal cardiovascular health by sex and region—a population-based study in Peru
Authors: Vera-Ponce Víctor Juan , Zuzunaga-Montoya Fiorella E. , Vásquez-Romero Luisa Erika Milagros , Loayza-Castro Joan A. , Orihuela Manrique Eder Jesús , Valladares-Garrido Mario J. , Gutierrez De Carrillo Carmen Inés 
Published: January 2025

IntroductionAttaining what the American Heart Association terms Ideal Cardiovascular Health (ICVH) is viewed as an essential objective for preventing cardiovascular diseases (CVD).ObjectiveTo determine the prevalence of ICVH, stratified by sex and region and its associated factors in the adult population of Peru.Materials and methodsAnalytical cross-sectional study. Data were obtained from the Life Stage Food and Nutrition Surveillance Survey (VIANEV). ICVH can be depicted vis-a-vis the seven metrics of the AHA: blood pressure levels, total cholesterol and glucose levels, smoking status, body mass index, physical activity levels, and dietary intake through the consumption of fruits and vegetables. The variable was categorized as deficient/moderate vs. ideal for regression analysis.ResultsOf the 863 participants examined, findings demonstrated that 38.01% had ICVH. The prevalence is trending lower in correlation with rising age and educational attainment levels and for those inhabitants residing at higher elevations. Likewise, statistically significant variations were observable concerning the prevalence of ICVH contingent on the region of residence and marital status, in particular amongst the feminine inhabitants.ConclusionsThese results demonstrate that the frequency of ICVH in the grown-up inhabitants of Peru is comparatively tiny. Things linked to a lesser frequency of ICVH involve increased age, a higher level of education, living at higher altitudes, and staying married to a woman. These discoveries underscore the need to implement prevention and treatment strategies for CVD distinct for each inhabitant team.
Identification of mitophagy-related key genes and their correlation with immune cell infiltration in acute myocardial infarction via bioinformatics analysis
Authors: Sheng Zulong , Zhang Rui , Ji Zhenjun , Liu Zhuyuan , Zhou Yaqing 
Published: January 2025

BackgroundAcute myocardial infarction (AMI), a subset of acute coronary syndrome, remains the major cause of mortality worldwide. Mitochondrial dysfunction is critically involved in AMI progression, and mitophagy plays a vital role in eliminating damaged mitochondria. This study aimed to explore mitophagy-related biomarkers and their potential molecular basis in AMI.MethodsAMI datasets (GSE24519 and GSE34198) from the Gene Expression Omnibus database were combined and the batch effects were removed. Differentially expressed genes (DEGs) in AMI were selected, intersected with mitophagy-related genes for mitophagy-related DEGs (MRDEGs), and then subjected to enrichment analyses. Next, the MRDEGs were screened using machine learning methods (logistic regression analysis, RandomForest, least absolute shrinkage and selection operator) to construct a diagnostic risk model and select the key genes in AMI. The diagnostic efficacy of the model was evaluated using a nomogram. Moreover, the infiltration patterns of different immune cells in two risk groups were compared. We also explored the interactions between the key genes themselves or with miRNAs/transcription factors (TFs) and drug compounds and visualized the protein structure of the key genes. Finally, we explored and validated the expression of key genes in plasma samples of patients with an AMI and healthy individuals.ResultsWe screened 28 MRDEGs in AMI. Based on machine learning methods, 12 key genes were screened for the diagnostic risk model, including AGPS, CA2, CAT, LTA4H, MYO9B, PRDX6, PYGB, SIRT3, TFEB, TOM1, UBA52, and UBB. The nomogram further revealed the accuracy of the model for AMI diagnosis. Moreover, we found a lower abundance of immune cells such as gamma delta T and natural killer cells in the high-risk group, and the expression of key genes showed a significant correlation with immune infiltration levels in both groups. Finally, 64 miRNA–mRNA pairs, 75 TF–mRNA pairs, 119 RNA-binding protein–mRNA pairs, and 32 drug–mRNA pairs were obtained in the interaction networks.ConclusionsIn total, 12 key MRDEGs were identified and a risk model was constructed for AMI diagnosis. The findings of this study might provide novel biomarkers for improving the detection of AMI.
Soil moisture partitioning strategies in blowouts in the Hulunbeier grassland and response to rainfall
Authors: Bao Zhixin , Yuan Limin , Meng Zhongju , Zhang Ezhen , Zhu Lei , Liu Jinwang 
Published: January 2025

IntroductionSoil moisture and soil water retention capacity are key influencing factors for the normal growth and development of vegetation. Understanding the dynamic change characteristics of soil moisture in blowouts and soil water retention capacity is of great significance for the management of blowouts.MethodsThis study employs drying and in situ monitoring methods to select typical blowouts in different regions (sand pits, fringe zones, sand accumulation zones, and sand-grass transition zones) on the Hulunbuir Grassland. A large area of natural grassland surrounding these regions was chosen as the control (CK). Soil moisture at depths of 20, 40, 60, 100 and 200 cm below the surface was measured along the soil profile using the ECH2O-10HS soil moisture automatic monitoring instrument. The HOBO-RG3-M self-recording rain gauge was used to monitor rainfall. Soil water storage, coefficient of variation, and Pearson’s correlation coefficient were calculated to study the differences in soil moisture and the dynamic change regularity in soil moisture under different rainfall conditions. This research provides important theoretical support for the soil moisture distribution and vegetation restoration in the blowouts of the Hulunbuir Grassland.Results and discussionThe volumetric water content of the soil in the blowouts was 15.95%, the volumetric soil water content in different parts of the soil varied from low to high as follows: sand pit-I &lt; sand-grass transition zone-IV &lt; fringe zone-II &lt; CK &lt; sand accumulation zone-III. The soil volumetric water content of the 0–40 cm soil layer of the blowout was higher than 17.47%, and the soil volumetric water content of the 40–200 cm soil layer ranged from 12.13% to 17.47%. The volumetric water content of soil in various parts of the blowouts under different rainfall amounts had significant differences, with rainstorms and heavy rainfall effectively recharging the blowouts to a depth of 200 cm, and the blowouts responded strongly to heavy rainfall (71.5 mm). A gradual recovery of the pre-rainfall volumetric soil moisture content was seen approximately a week after rainstorms. The water retention and storage capacity of blowout soils was significantly higher than that of CK, the soil water storage capacity of different zones ranked in descending order as the sand accumulation zone (1875.38 mm) &gt; edge zone (1373.22 mm) &gt; CK (1188.36 mm) &gt; sand pit (1000.39 mm) &gt; sand–grass transition zone (803.90 mm). The correlation coefficient of sand pits and sand cover was 0.5612, and that of sand accumulation zones and sand cover was 0.5845, which confirmed that sand cover enhanced the water retention capacity of the localized area of blowouts (sand accumulation zones).
Genetic variation in patent foramen ovale: a case-control genome-wide association study
Authors: Dong Bosi , Li Yajiao , Ai Fandi , Geng Jia , Tang Ting , Peng Wan , Tang Yusha , Wang Hui , Tian Zixuan , Bu Fengxiao , Chen Lei 
Published: January 2025

BackgroundPatent foramen ovale (PFO) is a congenital defect between the atria, resulting in abnormal hemodynamics. We conducted a genome-wide association study (GWAS) to identify common genetic variants associated with PFO.MethodsWe performed a whole genome sequencing in a discovery cohort of 3,227 unrelated Chinese participants screened for PFO via contrast transthoracic echocardiography (cTTE). Single-nucleotide polymorphisms (SNPs) associated with PFO were further validated by Sanger sequencing and subsequently were evaluated in a validation cohort. Expression quantitative trait loci (eQTL) analysis was conducted using the GTEx database. Single-cell sequencing analyses with pseudotime trajectory modeling were employed to evaluate their expression in human fetal hearts.ResultsThe case-control GWAS of discovery cohort ultimately included 517 cases and 517 demographically matched controls. Of the 7,040,407 variants assessed, we identified rs1227675732 (OR = 2.903; 95% CI, 1.961 to 4.297; p = 3.05 × 10−8), rs62206790 (OR = 2.780; 95% CI, 1.864 to 4.146; p = 2.02 × 10−7), rs879176184 (OR = 2.724; 95% CI, 1.822 to 4.073; p = 4.30 × 10−7) and rs13115019 (OR = 2.437; 95% CI, 1.702 to 3.488; p = 5.80 × 10−7) as high-risk variants for PFO, while rs57922961 (OR = 0.5081; 95% CI, 0.388 to 0.666; p = 6.82 × 10−7) was identified as protective variant. These variations were replicated in the validation cohort (111 cases and 152 controls). Single-cell sequencing showed that CNOT2, KCNMB4, MLLT10, IGBP1, and FRG1 were highly expressed with significant changes during heart development.ConclusionThe identification of susceptible loci for PFO might provide insights into the pathogenesis of PFO and contribute to understanding heart development.Clinical Trial Registrationhttps://www.chictr.org.cn/showproj.html?proj=40590, identifier ChiCTR1900024623.
Specific immune response to M. tuberculosis and ability to in vitro control mycobacterial replication are not impaired in subjects with immune-mediated inflammatory disease and tuberculosis infection
Authors: Farroni Chiara , Altera Anna Maria Gerarda , Salmi Andrea , Vanini Valentina , Cuzzi Gilda , Lindestam Arlehamn Cecilia S. , Sette Alessandro , Delogu Giovanni , Palucci Ivana , Sbarra Settimia , Aiello Alessandra , Picchianti-Diamanti Andrea , Gualano Gina , Palmieri Fabrizio , Goletti Delia , Petruccioli Elisa 
Published: January 2025

BackgroundSubjects with immune-mediated inflammatory diseases (IMID), such as rheumatoid arthritis, with tuberculosis infection (TBI), have a high probability of progressing to tuberculosis disease (TB). We aim to characterize the impact of IMID on the immune response to M. tuberculosis (Mtb) in patients with TBI and TB disease.MethodsWe enrolled TBI and TB patients with and without IMID. Peripheral blood mononuclear cells (PBMCs) were stimulated with Mtb-derived epitopes (MTB300). By flow-cytometry, we identified the Mtb-specific CD4+ T cells as cytokine-producing T cells or as CD25+ CD134+ CD4+ T cells. Memory and activation status of Mtb-specific T cells were assessed by evaluating: CD153, HLA-DR, CD45RA, CD27. Mycobacterial growth inhibition assay (MGIA) was used to evaluate the ability of PBMCs to inhibit mycobacteria growth. A long-term stimulation assay was used to detect a memory response.ResultsThe IMID status and therapy did not affect the magnitude of response to Mtb-antigen stimulation and the number of responders. TBI-IMID showed a cytokine profile like TBI and TB patients. The Mtb response of TBI-IMID patients was characterized by an effector memory and central memory phenotype as in TBI and TB groups. This memory phenotype allowed the increased IFN-γ production after 6 days of MTB300-stimulation. HLA-DR expression on Mtb-specific T cells was associated with TB, whereas CD153 was associated with TBI status. Finally, the TBI-IMID had an MGIA response like TBI and TB patients.ConclusionIMID condition does not affect key aspects of the immune response to Mtb, such as the cytokine response, memory and activation profile, and the ability to contain the mycobacteria replication. The immunological characterization of the fragile population of TBI-IMID patients is fundamental to understanding the correlation between protection and disease.
Effects of NK cell-related lncRNA on the immune microenvironment and molecular subtyping for pancreatic ductal adenocarcinoma
Authors: Li Jinze , Xia Chuqi , Li Yuxuan , Liu Hanhan , Gong Cheng , Liang Daoming 
Published: January 2025

BackgroundPatients with pancreatic ductal adenocarcinoma (PDAC) face a highly unfavorable outcome and have a poor response to standard treatments. Immunotherapy, especially therapy based on natural killer (NK) cells, presents a promising avenue for the treatment of PDAC.AimsThis research endeavor seeks to formulate a predictive tool specifically designed for PDAC based on NK cell-related long non-coding RNA (lncRNA), revealing new molecular subtypes of PDAC to promote personalized and precision treatment.MethodsUtilizing the Tumor Immune Single-cell Hub 2 platform, we discovered genes associated with NK cells in PDAC. We employed the TCGA-PAAD dataset to ascertain the expression profiles of these NK cell-related genes and to screen for lncRNAs correlated with NK cells. Subsequently, we utilized Cox regression analysis for hazard ratios and LASSO regression analysis to identify three NK cell-related lncRNAs that were used to develop a prognostic assessment model. The forecasting accuracy of this model was appraised using the ROC curve and validated using a test set and the complete dataset.ResultsSuccessful construction of a prognostic model comprising three lncRNAs was achieved, demonstrating good predictive efficiency in the training set, validation dataset, and the entire dataset. NK cells display robust interactions with malignant cells, CD8 T cells, and fibroblasts in the PDAC tumor microenvironment and participate in the transport of various signaling molecules and following immune responses in PDAC. According to the expression patterns of NK cell-related lncRNA, we labeled PDAC patients as four molecular subtypes, exhibiting significant differences in immune cell infiltration, drug sensitivity, and other aspects.ConclusionThis study Uncovered the activity of NK cells within PDAC, proposed an NK cell-related lncRNA model, and delineated new molecular subtypes, thereby providing targets for personalized therapy.