bluuebunny/crossref_metadata_2025_split huggingface dataset:
Driver Expectations of a Partial Driving Automation System in Relation to Branding and Training
Authors: Singer Jeremiah , Tefft Brian C , Benson Aaron , Jenness James W , Horrey William J 
Published: December 2022

Objective The current study examined whether differences in the branding and description or mode of training materials influence drivers’ understanding and expectations of a partial driving automation system. Background How technology is described might influence consumers’ understanding and expectations, even if all information is accurate. Method Ninety drivers received training about a real partial driving automation system with a fictitious name. Participants were randomly assigned to a branding condition (system named AutonoDrive, training emphasized capabilities; or system named DriveAssist, training emphasized limitations) and training mode (quick-start brochure; video; or in-person demonstration). No safety-critical information was withheld nor deliberately misleading information provided. After training, participants drove a vehicle equipped with the system. Associations of drivers’ expectations with branding condition and training mode were assessed using between-subjects comparisons of questionnaire responses obtained pre- and post-drive. Results Immediately after training, those who received information emphasizing the system’s capabilities had greater expectations of the system’s function and crash avoidance capability in a variety of driving scenarios, including many in which the system would not work, as well as greater willingness to utilize the system’s workload reduction benefits to take more risks. Most but not all differences persisted after driving the vehicle. Expectations about collision avoidance differed by training mode pre-drive but not post-drive. Conclusion Training that emphasizes a partial driving automation system’s capabilities and downplays its limitations can foster overconfidence. Application Accuracy of technical information does not guarantee understanding; training should provide a balanced view of a system’s limitations as well as capabilities.
Causality between Stock Market, Domestic and Global Economic Policy Uncertainty: Evidence from India
Authors: Aggarwal Khushboo , Saradhi V. Raveendra 
Published: December 2022

The aim of this article is to examine the relationship between the Indian stock market and the domestic and global economic policy uncertainty (EPU) during the period: January 2003–June 2020. The study employed unit root tests, Johansen cointegration test, vector error correction model (VECM), Granger causality test and impulse response function (IRF) for the analysis. This study finds the evidence for the presence of long-run equilibrium relationship between the stock market, domestic economic policy uncertainty (DEPU) and global economic policy uncertainty (GEPU) in India. The findings suggest that there is bidirectional causality between DEPU and stock market; however, no causality is found between GEPU and stock market in either direction. Also, it is found that there is bidirectional causality between DEPU and GEPU. The findings of the study may help policymakers to formulate decisive monetary and fiscal policies to achieve financial stability and are important for the financial investors and hedgers for portfolio allocations in India. JEL Codes: C32, D80, G12
Effect of Delayed Anterior Cruciate Ligament Reconstruction on Repair of Concomitant Medial Meniscus Tears in Young Athletes
Authors: Sorey Woody , Hagen Mia S. , Mand Simran , Sliepka Joey , Chin Kenneth , Schmale Gregory A. , Kweon Christopher , Gee Albert O. , Saper Michael G. 
Published: December 2022

Background: Delayed anterior cruciate ligament (ACL) reconstruction (ACLR) is associated with an increased risk of meniscal injury. Limited data are available regarding the relationship between surgical delay and meniscal repairability in the setting of ACLR in young patients. Purpose: To determine whether time from ACL injury to primary ACLR was associated with the incidence of medial and/or lateral meniscal repair in young athletes who underwent meniscal treatment at the time of ACLR. Study Design: Case-control study; Level of evidence, 3. Methods: Records were retrospectively reviewed for patients aged 13 to 25 years who underwent primary ACLR between January 2017 and June 2020 by surgeons from a single academic orthopaedic surgery department. Demographic data were collected, and operative reports were reviewed to document all concomitant pathologies and procedures. Univariable and multivariable logistic regression analyses were used to determine factors associated with meniscal repair, including time elapsed from ACL injury to surgery. Results: Concomitant meniscal tears were identified and treated in 243 of 427 patients; their mean age was 17.9 ± 3.3 years, and approximately half (47.7%) of patients were female. There were 144 (59.3%) medial tears treated and 164 (67.5%) lateral tears treated; 65 (26.7%) patients had both medial and lateral tears treated. Median time from ACL injury to ACLR was 2.4 months (interquartile range, 1.4-4.7 months). Adjusted univariate analysis showed a statistically significant correlation between medial meniscal repair and time to surgery, with a 7% decreased incidence of medial meniscal repair per month elapsed between injury and surgery (odds ratio, 0.93 per month; 95% CI, 0.89-0.98; P = .006). No similar relationship was found between lateral meniscal repair and time to surgery (odds ratio, 1.02; 95% CI, 0.99-1.06; P = .24). Conclusion: In the setting of concomitant ACL and meniscal injuries, surgical delay decreased the incidence of medial meniscal repair in young athletes by 7% per month from time of injury.
Fatigue resistance of orthotropic steel deck system with double-side welded rib-to-deck joint
Authors: Chen Jie , Wei Chuan , Zhao Yadang 
Published: December 2022

Fatigue cracking is a major problem in orthotropic steel decks (OSDs). This study investigated the fatigue resistance of an OSD system with novel double-side welded rib-to-deck joints. The fatigue strengths of the novel rib-to-deck and conventional rib-to-diaphragm joints were determined by fatigue testing two full-scale segmental models. A framework for evaluating the dominant crack mode of an OSD system was then developed and applied; the inner weld toe-deck crack and weld toe-rib wall crack were identified as the dominant crack modes of the double-side welded and rib-to-diaphragm joints, respectively. The nominal and hot spot stress fatigue strengths of the novel rib-to-deck joint were 90.53 and 108.95 MPa, respectively, whereas those of the rib-to-diaphragm joint were 44.27 and 98.66 MPa, respectively.
A qualitative study of unpaid caregivers of persons living with dementia and their interactions with health and community-based services and providers
Authors: Liebzeit Daniel , Fields Beth , Robinson Erin L , Jaboob Saida , Ashida Sato 
Published: December 2022

Background and Objectives Unpaid caregivers frequently feel underprepared and without adequate training, support, or access to services and resources to care for persons living with dementia. Health and community-based services and providers are critical to support persons living with dementia and their unpaid caregivers. The objective of this research is to understand how unpaid caregivers of persons living with dementia interact with health and community-based services and providers. Research Design and Methods We used a qualitative descriptive approach to analyze data from one-on-one semi-structured interviews with unpaid caregivers of persons living with dementia ( N = 25). We used framework analysis to organize themes and sub-themes within the systems approach to healthcare delivery. Findings Participants described a need for additional guidance, information, and support from health and community-based services and providers. This need was described by participants at 4 levels: individual, care team, organization, and political and economic environment. Participants were active in proposing potential solutions to challenges at all 4 levels, including improving provider awareness and training in dementia, opportunities to provide resources and referrals upon dementia diagnosis, a central clearinghouse for information and resources for persons living with dementia and caregivers, and opportunities to close service gaps. Discussion and Implications Findings of this study highlight the need for increased involvement of caregivers of persons living with dementia as community stakeholders for improving care and services. Recommendations to promote high quality care and better support unpaid caregivers by developing interventions to address multiple aspects of healthcare delivery are consistent with recent legislation and are important areas of future research.
Perception of Caring Behaviors and Associated Factors Among Nurses Working in Harar Hospitals, Eastern Ethiopia
Authors: Fikre Amelework , Egata Gudina , Abdisa Lemesa , Yadeta Elias , Eyeberu Addisu , Dheresa Merga 
Published: December 2022

Introduction Perception of caring behavior is very necessary to enhance human life, and the provision of health care, and is considered a basic key in the provision of healthcare facilities. Even though all nursing interventions are verified through nurse caring behaviors, poor perception of caring behaviors reduces the well-being of the patients. Objective The objective of this study was to assess the perception of caring behaviors and associated factors among nurses working in Harar Hospitals, Eastern Ethiopia Methods An institutional-based cross-sectional study was conducted among 465 randomly selected nurses. Data were collected by using a pretested and structured self-administered questionnaire. Descriptive statistics including frequency table, mean, standard deviation, and percentage were employed. Binary logistic regression analysis was used to identify factors associated with the good perception of caring behaviors. Variables with a p-value of < .05 were considered statistically significant. Results This study revealed that the proportion of nurses who had a good perception of caring behaviors was found to be 63.4% (95% CI: 58.5–68.2%). High-level education (AOR = 2.98, 95% CI: 1.28–4.34), low workload (AOR = 1.22, 95% CI: 1.16–3.05), satisfied with a job (AOR = 1.92, 95% CI: 1.42–4.01), good relation with coworkers (AOR = 4.56, 95% CI: 2.15–9.67) were significantly associated with a good perception of caring behaviors. Conclusions This study reported that the proportion of nurses who had a good perception of caring behaviors was 63.4%. Educational level, workload, job satisfaction, and joint participation in the decision-making process were factors significantly associated with good perceptions of nurses caring behavior. Therefore, nurses caring behavior is enhanced by increasing educational levels, creating a conducive working environment, and making nursing active in the decision-making process to improve the perception of nurses toward nurses caring behavior.
Nitrate adsorption characteristics of synthesized allophanes with various chemical compositions
Authors: Ohashi Fumihiko 
Published: December 2022

AbstractAllophane-related aluminium silicates with various chemical compositions were synthesized using a hydrothermal reaction with inorganic reagents as starting solutions. The X-ray diffraction traces of the synthesized allophanes showed broad reflections centred at 0.34 and 0.23 nm, which were attributed to the imogolite-like structure of the allophanes. Energy-dispersive X-ray measurements confirmed that the Al-rich materials were synthesized as targeted. The specific surface area of the synthesized allophanes was 313–500 m2 g–1, which is greater than that of a natural allophane (248 m2 g–1). The amount of nitrate adsorbed on the synthesized allophanes tended to increase as the Al content increased. The maximum amount of nitrate adsorbed was 2.07 mmol g–1 at pH ~2, which was comparable to that of a common anion-exchange material. A possible adsorption mechanism for nitrate at lower pH levels is the weak NO3– interaction of the positively charged surface of Al-OH2+ sites.
The proteomic analysis of bovine embryos developed in vivo or in vitro reveals the contribution of the maternal environment to early embryo
Authors: Banliat Charles , Mahé Coline , Lavigne Régis , Com Emmanuelle , Pineau Charles , Labas Valérie , Guyonnet Benoit , Mermillod Pascal , Saint-Dizier Marie 
Published: December 2022

Abstract
Background
Despite many improvements with in vitro culture systems, the quality and developmental ability of mammalian embryos produced in vitro are still lower than their in vivo counterparts. Though previous studies have evidenced differences in gene expression between in vivo- and in vitro-derived bovine embryos, there is no comparison at the protein expression level.

Results
A total of 38 pools of grade-1 quality bovine embryos at the 4–6 cell, 8–12 cell, morula, compact morula, and blastocyst stages developed either in vivo or in vitro were analyzed by nano-liquid chromatography coupled with label-free quantitative mass spectrometry, allowing for the identification of 3,028 proteins. Multivariate analysis of quantified proteins showed a clear separation of embryo pools according to their in vivo or in vitro origin at all stages. Three clusters of differentially abundant proteins (DAPs) were evidenced according to embryo origin, including 463 proteins more abundant in vivo than in vitro across development and 314 and 222 proteins more abundant in vitro than in vivo before and after the morula stage, respectively. The functional analysis of proteins found more abundant in vivo showed an enrichment in carbohydrate metabolism and cytoplasmic cellular components. Proteins found more abundant in vitro before the morula stage were mostly localized in mitochondrial matrix and involved in ATP-dependent activity, while those overabundant after the morula stage were mostly localized in the ribonucleoprotein complex and involved in protein synthesis. Oviductin and other oviductal proteins, previously shown to interact with early embryos, were among the most overabundant proteins after in vivo development.

Conclusions
The maternal environment led to higher degradation of mitochondrial proteins at early developmental stages, lower abundance of proteins involved in protein synthesis at the time of embryonic genome activation, and a global upregulation of carbohydrate metabolic pathways compared to in vitro production. Furthermore, embryos developed in vivo internalized large amounts of oviductin and other proteins probably originated in the oviduct as soon as the 4–6 cell stage. These data provide new insight into the molecular contribution of the mother to the developmental ability of early embryos and will help design better in vitro culture systems.
Role of exogenous abscisic acid in freezing tolerance of mangrove Kandelia obovata under natural frost condition at near 32°N
Authors: Liu Xing , Lu Xiang , Yang Sheng , Liu Yu , Wang Wenqing , Wei Xin , Ji Hongjiu , Zhang Bo , Xin Wenzhen , Wen Junxiu , Wang Jinwang , Chen Qiuxia 
Published: December 2022

Abstract
Background
Mangroves possess substantial ecological, social, and economic functions in tropical and subtropical coastal wetlands. Kandelia obovata is the most cold-resistance species among mangrove plants, with a widespread distribution in China that ranges from Sanya (18° 12′ N) to Wenzhou (28° 20′ N). Here, we explored the temporal variations in physiological status and transcriptome profiling of K. obovata under natural frost conditions at ~ 32oN, as well as the positive role of exogenous abscisic acid (ABA) in cold resistance.

Results
The soluble sugar (SS) and proline (Pro) functioned under freezing stress, of which SS was more important for K. obovata. Consistently, up-regulated DEGs responding to low temperature were significantly annotated to glycometabolism, such as starch and sucrose metabolism and amino sugar and nucleotide sugar metabolism. Notably, the top 2 pathways of KEGG enrichment were phenylpropanoid biosynthesis and flavonoid biosynthesis. For the antioxidant system, POD in conjunction with CAT removed hydrogen peroxide, and CAT appeared to be more important. The up-regulated DEGs responding to low temperature and ABA were also found to be enriched in arginine and proline metabolism, starch and sucrose metabolism, and peroxisome. Moreover, ABA triggered the expression of P5CS and P5CR, but inhibited the ProDH expression, which might contribute to Pro accumulation. Interestingly, there was no significant change in malondialdehyde (MDA) content during the cold event (P > 0.05), suggesting foliar application of ABA effectively alleviated the adverse effects of freezing stress on K. obovata by activating the antioxidant enzyme activity and increasing osmolytes accumulation, such as Pro, and the outcome was proportional to ABA concentration.

Conclusions
This study deepened our understanding of the physiological characters and molecular mechanisms underlying the response of K. obovata to natural frost conditions and exogenous ABA at the field level, which could provide a sound theoretical foundation for expanding mangroves plantations in higher latitudes, as well as the development coastal landscape.
A survey of the full-length transcriptome of Gracilariopsis lemaneiformis using single-molecule long-read sequencing
Authors: Chen Xiaojiao , Tang Yue Yao , Yin Haodong , Sun Xue , Zhang Xiaoqian , Xu Nianjun 
Published: December 2022

Abstract
Background
Posttranscriptional processing of precursor mRNAs contributes to transcriptome and protein diversity and gene regulatory mechanisms in eukaryotes. However, this posttranscriptional mechanism has not been studied in the marine macroalgae Gracilariopsis lemaneiformis, which is the most cultivated red seaweed species in China.

Results
In the present study, third-generation sequencing (Pacific Biosciences single-molecule real-time long-read sequencing, SMRT-Seq) was used to sequence the full-length transcriptome of G. lemaneiformis to identify alternatively spliced transcripts and alternative polyadenylation (APA) sites in this species. RNAs were isolated from G. lemaneiformis under various treatments including abiotic stresses and exogenous phytohormones, and then equally pooled for SMRT-Seq. In summary, 346,544 full-length nonchimeric reads were generated, from which 13,630 unique full-length transcripts were obtained in G. lemaneiformis. Compared with the known splicing events in the gene models, more than 3000 new alternative splicing (AS) events were identified in the SMRT-Seq reads. Additionally, 810 genes were found to have poly (A) sites and 91 microRNAs (miRNAs), 961 long noncoding RNAs and 1721 novel genes were identified in G. lemaneiformis. Moreover, validation experiments showed that abiotic stresses and phytohormones could induce some specific AS events, especially intron retain isoforms, cause some alterations to the relative ratios of transcripts annotated to the same gene, and generate novel 3′ ends because of differential APA. The growth of G. lemaneiformis was inhibited by Cu stress, while this inhibition was alleviated by ACC treatment. RNA-Seq analysis further revealed that 211 differential alternative splicing (DAS) events and 142 DAS events was obtained in CK vs Cu and Cu vs Cu + ACC, respectively, suggesting that AS of functional genes could be regulated by Cu stress and ACC. Compared with Cu stress, the expression of transcripts with DAS events mainly involved in the carbon fixation in photosynthetic organisms and oxidative phosphorylation pathway was upregulated in Cu + ACC treatment, revealing that ACC alleviated the growth inhibition by Cu stress by increasing carbon fixation and oxidative phosphorylation.

Conclusions
Our results provide the first comprehensive picture of the full-length transcriptome and posttranscriptional mechanism in red macroalgae, including transcripts that appeared in the presence of common abiotic stresses and phytohormones, which will improve the gene annotations of Gracilariopsis and contribute to the study of gene regulation in this important cultivated seaweed.
Transcriptome reveals insights into biosynthesis of ginseng polysaccharides
Authors: Fang Xiaoxue , Wang Huaying , Zhou Xinteng , Zhang Jing , Xiao Hongxing 
Published: December 2022

Abstract
Background

Ginseng polysaccharides, have been used to treat various diseases as an important active ingredient. Nevertheless, the biosynthesis of ginseng polysaccharides is poorly understood. To elucidate the biosynthesis mechanism of ginseng polysaccharides, combined the transcriptome analysis and polysaccharides content determination were performed on the roots, stems, and leaves collected from four cultivars of ginseng.

Results
The results indicated that the total contents of nine monosaccharides were highest in the roots. Moreover, the total content of nine monosaccharides in the roots of the four cultivars were different but similar in stems and leaves. Glucose (Glc) was the most component of all monosaccharides. In total, 19 potential enzymes synthesizing of ginseng polysaccharides were identified, and 17 enzymes were significantly associated with polysaccharides content. Among these genes, the expression of phosphoglucomutase (PGM), glucose-6-phosphate isomerase (GPI), UTP–glucose-1-phosphate uridylyltransferase (UGP2), fructokinase (scrK), mannose-1-phosphate guanylyltransferase (GMPP), phosphomannomutase (PMM), UDP-glucose 4-epimerase (GALE), beta-fructofuranosidase (sacA), and sucrose synthase (SUS) were correlated with that of MYB, AP2/ERF, bZIP, and NAC transcription factors (TFs). These TFs may regulate the expression of genes involved in ginseng polysaccharides synthesis.

Conclusion
Our findings could provide insight into a better understanding of the regulatory mechanism of polysaccharides biosynthesis, and would drive progress in genetic improvement and plantation development of ginseng.