bluuebunny/crossref_metadata_2025_split huggingface dataset:
Antiproliferative activity and apoptosis-inducing mechanism of Curcuma longa (Turmimax®) on Hela cell lines
Authors: H.M Firoz , S Nanjundaiah , C.T Sadashiva , Benny Neethumol , Y Rashmi , A.K Sreedrisya 
Published: February 2023

Abstract
Turmeric's main ingredient, curcumin (diferuloylmethane), is a polyphenol obtained from the Curcuma longa plant. Since curcumin is nontoxic and has a wide range of medicinal qualities, including antioxidant, analgesic, anti-inflammatory, and antibacterial action, it has been widely employed in Ayurvedic medicine for ages. Curcumin has recently been discovered to have anti-cancer properties through its impact on numerous biological pathways that are involved in carcinogenesis, metastasis, tumorigenesis, cell cycle regulation, mutagenesis, and oncogene expression. In this study, we determined the antiproliferative activity and apoptosis-inducing mechanism of C. longa (Turmimax®) on human cancer cells. The cytotoxic effect was evaluated against HeLa cell lines using the MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay. A flow cytometric analysis was performed to detect apoptotic cell death. Turmimax® exhibits promising properties as a potential anti-cancer therapeutic agent in human cervical adenocarcinomas and possibly other cancer types, with an IC50 value of 87.89µg/mL. In HeLa cells treated with Turmimax®, cell cycle arrest was seen at the G0/G1 and S phases. By inducing apoptosis and increasing the number of apoptotic cells in a dose-dependent manner, the experimental data suggest that Turmimax® has considerable promise in the prevention and treatment of cancer.
Pathway Engineering of Multicomponent Self-Assembly in M12L24 Nanospheres using Pseudorotaxanes
Authors: Bouwens Tessel , Bobylev Eduard , Poole David , Mathew Simon , Reek Joost 
Published: February 2023

Abstract
Selective formation of multicomponent structures via the self-assembly of numerous building blocks is ubiquitous in biological systems but challenging to emulate synthetically. The energy landscape for product formation broadens with increasing number of components, introducing kinetic intermediates with trap-state ability. Therefore, pathway engineering becomes an essential tool to navigate these complex kinetic landscapes and push self-assembly to greater limits. Here, we report an example of pathway engineering in the self-assembly of cuboctahedral M12L24 nanospheres through pseudorotaxane formation to the exo-functionalized ligands. Without ring in solution for pseudorotaxane formation, a Pdx–Ly polymer resting-state is rapidly formed. Solutions with ring exhibit rapid nanosphere formation from small Pdx–Ly oligomers, with Pdx–Ly polymer formation bypassed. The threading of ring to an exo-functionalized ligand therefore directs the self-assembly pathway toward nanosphere formation. As the process of pseudorotaxane formation increases rate of nanosphere formation, the ring effectively acts as a supramolecular catalyst for nanosphere self-assembly.
Analysis of the moderating effect on cultivated land and poverty incidence based on geographical weighted regression
Authors: Feng Duxian , He Yawen , Ren Zhoupeng 
Published: February 2023

Abstract
Despite economic progress and reducing poverty, poverty remains one of the world’s major problems. Cropland is fundamental for agricultural production and social development in rural areas. Previous research has theoretically discussed the relationship between cultivated land and poverty incidence from the perspective of spatial poverty. The linkage between arable land use and poverty incidence is ambiguous, and other regional conditions’ moderation effect has seldom been investigated. Seven geographical indicators and four interaction terms were used to explore the moderating effect of other factors between cultivated land and poverty incidence in Linyi based on geographical weighted regression. The results demonstrate-d that environmental factors moderated the relationship between cultivated land resources and the incidence of poverty. Specifically, we found spatial nonstationarity of moderation effect associated with the elevation difference. The moderation effect of road and accessibility with cultivated land presented different directions and strengths of the association in two datasets grouped by the median of elevation. Based on these findings, we expect this paper to provide valuable insights into analyzing the poverty of complexity of interaction with the environment.
Comparison of Prognosis Between Patients with Acute Myocardial Infarction Treated with Ticagrelor and Clopidogrel in the Emergency Department: A Propensity Score Matched Retrospective Cohort Study
Authors: Huang Po-Yao , Shih Hong-Mo , Huang Szu-Wei , Pan Yan-Cheng , Huang Fen-Wei , Chen Wei-Kung , Yu Shao-Hua 
Published: February 2023

Abstract
Background
Dual antiplatelet therapy (DAPT) is a standard treatment option for acute myocardial infarction (AMI). The difference between the efficacy of ticagrelor and clopidogrel in the emergency department (ED) before percutaneous coronary intervention (PCI) remains unknown. The present study compared the in-hospital major adverse cardiovascular event (MACE) rates between patients with AMI treated with clopidogrel and those treated with ticagrelor in the ED before PCI.
Methods
We retrospectively collected the data of patients diagnosed as having AMI in the ED. Patients were only included if they had successfully received complete DAPT with aspirin and ticagrelor/clopidogrel in the ED and had undergone PCI. The patients were divided into 2 groups according to their DAPT regimen. The primary outcome was the rate of in-hospital MACEs. The secondary outcomes included an unexpected return to the ED within 72 h, readmission within 14 d, and revascularization.
Results
1836 patients were enrolled. Patients in the ticagrelor group had a lower in-hospital MACE rate (3.01% versus 7.51%, P < .001) and in-hospital mortality rate (2.15% versus 5.70%, P < .001) than those in the clopidogrel group. Multivariate logistic regression analysis revealed ticagrelor was independently associated with a lower risk of in-hospital MACEs (odds ratio [OR]: 0.53, 95% CI: 0.32–0.88, P = .013). By conditional logistic regression with a propensity score matching, similar results were found (OR 0.41, 95% CI: 0.18–0.91, P = .028)
Conclusion
DAPT with ticagrelor and aspirin in the ED before PCI is associated with a lower in-hospital MACE rate among patients with AMI.
A Model for Early Detection of Stuck Pipe Using Random Forest Algorithm
Authors: Duan Jiehong , Tian Yujie , Long Ethan , Long Wei 
Published: February 2023

Abstract

Stuck pipe remains as one of the major risks in the drilling operation that could cause significant non-productive time (NPT). The earlier stuck pipe risk is predicted and mitigated, the higher the chance of preventing its occurrence in the first place. In this study, a new model was proposed using random forest (RF) algorithm together with sliding window technique to quickly detect the symptoms of potential stuck pipe, by capturing the hidden pattern and trends of certain real-time drilling parameters (hook load, Torque, standpipe pressure and weight on bit) fall outside of the boundaries calculated dynamically from the Pauta Criterion. After being trialed on wells for both offshore and unconventional, the developed model is able to give the early indication of an impending stuck pipe from 3 minutes to 43 minutes ahead of its actual occurrence, thus leave the rig crew valuable time window to take proper interventions.
Prevalence of dry eye disease among IT students in Pakistan
Authors: Sarwat Sidra , Mamoon Izma , Ayub Fareeha 
Published: February 2023

Abstract
Background:
Dry eye disease (DED) is a common tear film disorder caused by decreased tear production or increased tear evaporation. The increased use of computers in the workplace has brought about the development of ocular health concerns, including dry eye disease. Therefore, this study was designed to determine the prevalence of DED associated with computer work and recommend preventing or reducing its development. This study investigated the prevalence of DED among IT students in Dera Ghazi Khan.
Methods:
A cross-sectional descriptive study was conducted among 327 undergraduate students of the IT department in the district of Dera Ghazi khan over five months from September 2021 to January 2022. DED was assessed subjectively with Ocular Surface Disease Index (OSDI). Data were analysed using SPSS version 21. The quantitative analysis was presented as mean and standard deviation, and qualitative variables were introduced in frequency and percentages. The chi-square test (Fisher’s exact test) was used to study the significance of associations between dependent and independent variables.
Results:
The mean age of the participants was 20.25 ± 1.305 years (range, 17–25). The prevalence of DED among computer programmers was 47.7%. This study showed that gender (p = 0.31), contact lens usage (p = 0.64), and duration of time spent on computer display units (p = 0.47) were not significant determinants of DED among computer programmers.
Conclusion:
DED is prevalent among IT students, along with different symptoms. However, further studies are required to adequately measure the prevalence of DED during longer exposure time to computer screens and explore environmental risk factors.
Compliance with COVID-19 public health and social preventive measures among Employees of Jimma zone, Ethiopia: Application of Health Belief Model
Authors: Teshome Firanbon , Kebede Ayantu , Kebede Alemi , Belina Sena , Tadele Afework 
Published: February 2023

Abstract
Background
COVID-19 is still threatening the world and has health, social, economic and political crisis. COVID-19 preventive measures are of having paramount importance for the disease mitigation. Governmental and private employees’ compliance with COVID-19preventive measures are highly important in influencing their friends, spouse, families and community at the large to adhere to the preventive measures.
Objectives
Compliance with COVID-19 preventive measures and associated factors among employees of Seka and Kersa districts, Jimma zone, Ethiopia, 2020.
Methods
A cross-sectional study was conducted from 4–22 January 2021. A total of 395 employees from randomly selected of public and private institutions were participated in the study. The data were collected using a pre-tested structured tool. The collected data were entered to EPI data manager version 4.6 and exported to Stata version 16.0 for analyses. Descriptive analyses were performed as necessary. Binary and multivariable logistic regression models were fitted to identify determinants of compliance with COVID-19 preventive measures. Adjusted odds ratios with 95% confidence intervals were used to determine the presence and strength of association.
Results
This study showed that nearly three fourth (74.68%) of employees were compliant with the COVID-19 preventive measures. Being employees of private institution (adjusted β = 3.12; 95% CI 0.31 to 5.94; p = 0.030), having history of chronic illness (adjusted β = 4.06; 95% CI 1.58 to 6.54; p = 0.001), perceived susceptibility to COVID-19 (adjusted β = 0.38; 95% CI 0.16 to 0.61; p = 0.001), self-efficacy to utilize the preventive measures (adjusted β = 0.70; 95% CI 0.54 to 0.86; p < 0.001) and existence of reminders/cues to utilize the preventive measures (adjusted β = 0.47; 95% CI 0.27 to 0.67; p < 0.001) had statistically significant association with COVID-19 preventive measures.
Conclusions
We found that one fourth of the employees were non-compliant with COVID-19 preventive measures. Type of working institution, respondents’ history of chronic illness, perceived susceptibility to COVID-19, self-efficacy to utilize the preventive measures and existence of reminders /cues to utilize the preventive measures predicted employees’ compliance with COVID-19 preventive measures. Therefore, behaviour change communication and interventions strategies guided by health belief model need to be considered by stakeholders.
The effectiveness of the prevention and control methods applied towards the elimination of malaria in Botswana
Authors: Saudu Collen , Mokgothu Thuso Solomon , Ntebela Davies S , Bakani Lebogang , Molongwane Charles 
Published: February 2023

Abstract
Background
In 2008, the Ministry of Health (MOH) in Botswana was inspired by an overall decline of malaria prevalence within the population from 4.2% in 2000 to less than 1% in 2008. These results motivated the Government of Botswana to re-orientate strategy towards the elimination of malaria by 2018. Heavy rainfalls in 2017 led to an epidemic which caused a huge setback to their goal. This setback was especially due to the malaria cases reported in the South-East District, a region not classified as endemic in Botswana. This study examines the progress, effectiveness, and challenges of prevention and control methods for the successful elimination of malaria in Botswana.
Materials & Methods
Retrospective data about cases of malaria in Botswana covering endemic districts, and data about Indoor Residual Spray (IRS), Long -Lasting Insecticides Nets (LLINs) and larviciding from 2000 to 2018 were obtained from the Botswana MOH. Rainfall data from the Department of Metrological Services were also obtained. Intervention datasets were compared against rainfall amount and number of cases to establish their relationship.
Results
There is a significant difference between percentage of malaria incidence and percentage of mean annual rainfall in Botswana. A moderate positive correlation of 0.49 was reported with a p-value of 0.108 and t-value of 1.7652. Intensifying interventions in Botswana had no effect on number of cases based on this study. IRS coverage and intensity increased over years from 52.02% (2011) to 84.70% (2017), yet one heavy rainfall season resulted in increased cases of malaria in Botswana.
Conclusions
Improved forecasting can be used to predict malaria in Botswana, indicating malaria prevalence in Botswana is heavily dependent on the climate. IRS, LLINs, and larviciding should be carried out at the right time, not after mosquito numbers have increased and consistently without failure, to eliminate malaria. The steep decline in cases of malaria from 2000 to 2013 was likely due to the dry weather, not control methods applied.
Reference-quality bat genomes illuminate adaptations to viral tolerance and disease resistance
Authors: Morales Ariadna , Dong Yue , Brown Thomas , Baid Kaushal , Kontopoulos Dimitrios , Gonzalez Victoria , Huang Zixia , Ahmed Alexis , Hilgers Leon , Winkler Sylke , Hughes Graham , Li Xiaomeng , Kirilenko Bogdan , Devanna Paolo , Lama Tanya , Nissan Yomiran , Pippel Martin , Dávalos Liliana , Vernes Sonja , Puechmaille Sebastien , Rossiter Stephen , Yovel Yossi , Prescott Joe , Kurth Andreas , Ray David , Lim Burton , Myers Eugene , Teeling Emma , Banerjee Arinjay , Irving Aaron , Hiller Michael 
Published: February 2023

Abstract

Bats carry viruses that can cause severe disease in other mammals. Asymptomatic infections in bats suggest limited tissue-damaging inflammation and immunopathology. To investigate the genomic basis of disease resistance, the Bat1K project generated reference-quality genomes of ten bat species. A systematic analysis showed that signatures of selection in immune genes are more prevalent in bats compared with other mammals. We found an excess of immune gene adaptations in the ancestral Chiroptera and many descending bat lineages, highlighting viral entry and detection factors, and regulators of antiviral and inflammatory responses. ISG15, an antiviral gene contributing to hyperinflammation during COVID-19, exhibits a deletion of a cysteine, required for homodimer formation, in rhinolophid and hipposiderid bats. Cellular infection experiments showed enhanced intracellular protein conjugation of bat ISG15 and lack of secretion into extracellular space, where human ISG15 stimulates inflammation. Our work highlights molecular mechanisms contributing to viral tolerance and disease resistance in bats.
Salinity tolerance screening in Iranian and Afghan melons (Cucumis melon) based on several associated morphological and physiological traits
Authors: Hekmat Hikmatullah , Haghighi Maryam , Eshghizade Hamid Reza , Banitalebi Golnoosh 
Published: February 2023

Abstract
This study was carried out to investigate the effect of salinity on some physiological and morphological traits of native Iranian and Afghan melon cultivars using a split-plot experiment with a randomized complete block design and three replications. Two salinity levels (2 and 8 dSm-1 NaCl) and 39 cultivars from Iran and Afghanistan were utilized. This study was carried out to investigate the effect of salinity stress on growth and yield and recommend the tolerant genotype(s). PCA comparisons were done between biochemical and morphological parameters. The sensitive and tolerant cultivar was chosen based on proximity to high yield, morphological characteristics, and distance from stress indices. The biplot results showed a high correlation between vitamin C traits with soluble solids, proline, and relative water content and a negative correlation with Fv/Fm ratio. These indices are good indicators for identifying saline resistance cultivars. Salinity stress increased electrolyte leakage, proline concentration, total antioxidant activity, Na content, vitamin C, organic acid, and total soluble solids. In addition, salinity decreased the yield, mean fruit weight, firmness, fruit length, fruit width, internal cavity length, internal cavity width, flesh thickness and fruit peel thickness, Fv/Fm ratio, greenness index, relative water content, leaf K.. The highest concentrations of Na and K were found in the G-SHI cultivar under salinity, while the highest concentrations of Na and K were found in the Tork cultivar under non-saline conditions. Based on the results, two types of Tork and Zank melon were recommended to plant in saline conditions.
Estimation of whole blood HbA1c by aptamer based colorimetric assay using gold nanoparticles- A Novel Aproach
Authors: Devi Rooma , Chauhan Aman , Kumar Prabhat , Pundir C.S. 
Published: February 2023

Abstract
Background
Diabetes mellitus is a chronic metabolic disease that can cause several complications representing one of the most important health concerns now a-days. Aptamers are short single stranded DNA (ssDNA) or RNA sequences and have been recently appeared as novel recognition receptors which can be used as alternative to antibodies in biosensing devices.
Materials and Methods
Characterization of the selected aptamer and its Immobilization with gold nanoparticles (AuNPs) which is used for colorimetric detection of glycated hemoglobin (HbA1C).
Conclusion
The proposed method is highly sensitive & specific and could be used for detection of whole blood HbA1c levels.
Scheduling Approach of Automatic Guided Vehicle Considering Equipment Failure and Power Management
Authors: Fan Guoliang , Jiang Zuhua 
Published: February 2023

Abstract
Predictive management on power consumption during static scheduling of outfitting automatic transportation equipment is very difficult. In addition, dynamic scheduling has poor response ability to sudden failures. To solve this problem, this paper proposes an AGV (Automatic Guided Vehicle) scheduling approach for outfitting warehouse considering equipment failure and power management. Firstly, based on the power consumption rate function, the power consumption model of AGV for transport task is established. According to the departure power and task consumption power, the charging time and the return time of the AGV are calculated. Secondly, the optimization model of AGV scheduling is established with the goal of minimizing the total transport time. Further, the variable definitions of overhaul and minor repair for AGV are made, and the scheduling strategy for sudden failure is proposed. Finally, an optimization algorithm based on power consumption and failure maintenance for outfitting vehicle scheduling is developed to solve the optimization model in a case. The proposed method can pre plan the charging time according to the vehicle power consumption. Rescheduling is carried out for sudden failure and charging return to improve the dynamic response capability of outfitting transport vehicles.
Design, synthesis, and biological evaluation of quinoline (quinolinone) derivatives as NADPH oxidase (NOX) inhibitors
Authors: Zhang Lei , Wu Siming , Yang Xinliang , Yi Rui , Hu gaoyun , Li Qianbin , Chen Zhuo 
Published: February 2023

Abstract
NADPH oxidase (NOX) is the only enzyme in the human body that can directly produce reactive oxygen species. Recent studies have shown that NADPH oxidase is a very potential target for the treatment of Diabetic Nephropathy (DN). Here, a series of quinoline (quinolinone) derivatives were designed and synthesized based on pharmacophore modelling. And the antiproliferative effect against NRK-49F cell-line was evaluated as their potential anti-DN activity. 19d, the most potent compounds with an effective cell proliferation inhibitory activity (IC50 = 5.25 uM), showed a promising inhibitory activity on NOXs (IC50 = 1.12 µM). The 3D-QSAR model based on the anti-proliferation activity of NRK-49F exhibited the good predictive ability. In conclusion, 19d may have therapeutic effects on diabetic nephropathy like other NOX inhibitors in clinical trials.
Plasma cell-free DNA in patients with acute promyelocytic leukemia treated with arsenic trioxide
Authors: Fujihara Junko , Nishimoto Naoki , Takeshita Haruo 
Published: February 2023

Abstract
Cell-free DNA (cfDNA) is free DNA found in circulating blood that originates from apoptosis or necrosis, and elevated cfDNA concentrations have been reported in cancers and other diseases. In this study, the concentrations and fragment distributions of plasma cfDNA were investigated in elderly (n = 1) and pediatric (n = 1) patients with acute promyelocytic leukemia (APL) treated with arsenic trioxide (ATO). A slight increase in cfDNA concentrations was observed in the APL patients compared with healthy controls. In the elderly APL patient, the plasma cfDNA concentration increased with treatment duration, and significant positive correlations were observed between the plasma total arsenic concentration and cfDNA concentration during the first consolidation therapy. Ladder fragments (150–200bp, 300–400 bp, and 500–600 bp) were observed in a portion of plasma cfDNA from both APL patients. Moreover, APL-related gene mutations (FMS-like tyrosine kinase 3 internal tandem duplication, GATA-binding protein 2 gene, Janus-associated kinase 2 gene, Wilms’ tumor 1 gene, Kirsten-ras gene, and neuroblastoma-ras gene) were successfully genotyped from plasma cfDNA by using polymerase chain reaction-based methods. The findings of this study provide fundamental information on cfDNA from APL patients treated with ATO.