bluuebunny/crossref_metadata_2025_split huggingface dataset:
Integrative multi-omics analysis reveals novel idiopathic pulmonary fibrosis endotypes associated with disease progression
Authors: Ruan Peifeng , Todd Jamie L , Zhao Hongyu , Liu Yi , Vinisko Richard , Soellner Julia F. , Schmid Ramona , Kaner Robert J. , Luckhardt Tracy R. , Neely Megan L. , Noth Imre , Porteous Mary , Raj Rishi , Safdar Zeenat , Strek Mary E , Hesslinger Christian , Palmer Scott M. , Leonard Thomas B. , Salisbury Margaret L. 
Published: June 2023

AbstractBackgroundIdiopathic pulmonary fibrosis (IPF) is characterized by the accumulation of extracellular matrix in the pulmonary interstitium and progressive functional decline. We hypothesized that integration of multi-omics data would identify clinically meaningful molecular endotypes of IPF.MethodsThe IPF-PRO Registry is a prospective registry of patients with IPF. Proteomic and transcriptomic (including total RNA [toRNA] and microRNA [miRNA]) analyses were performed using blood collected at enrollment. Molecular data were integrated using Similarity Network Fusion, followed by unsupervised spectral clustering to identify molecular subtypes. Cox proportional hazards models tested the relationship between these subtypes and progression-free and transplant-free survival. The molecular subtypes were compared to risk groups based on a previously described 52-gene (toRNA expression) signature. Biological characteristics of the molecular subtypes were evaluated via linear regression differential expression and canonical pathways (Ingenuity Pathway Analysis [IPA]) over-representation analyses.ResultsAmong 232 subjects, two molecular subtypes were identified. Subtype 1 (n = 105, 45.3%) and Subtype 2 (n = 127, 54.7%) had similar distributions of age (70.1 +/- 8.1 vs. 69.3 +/- 7.6 years; p = 0.31) and sex (79.1% vs. 70.1% males, p = 0.16). Subtype 1 had more severe disease based on composite physiologic index (CPI) (55.8 vs. 51.2; p = 0.002). After adjusting for CPI and antifibrotic treatment at enrollment, subtype 1 experienced shorter progression-free survival (HR 1.79, 95% CI 1.28,2.56; p = 0.0008) and similar transplant-free survival (HR 1.30, 95% CI 0.87,1.96; p = 0.20) as subtype 2. There was little agreement in the distribution of subjects to the molecular subtypes and the risk groups based on 52-gene signature (kappa = 0.04, 95% CI= -0.08, 0.17), and the 52-gene signature risk groups were associated with differences in transplant-free but not progression-free survival. Based on heatmaps and differential expression analyses, proteins and miRNAs (but not toRNA) contributed to classification of subjects to the molecular subtypes. The IPA showed enrichment in pulmonary fibrosis-relevant pathways, including mTOR, VEGF, PDGF, and B-cell receptor signaling.ConclusionsIntegration of transcriptomic and proteomic data from blood enabled identification of clinically meaningful molecular endotypes of IPF. If validated, these endotypes could facilitate identification of individuals likely to experience disease progression and enrichment of clinical trials.Trial registrationNCT01915511
Risk of drug use during pregnancy: master protocol for living systematic reviews and meta-analyses performed in the metaPreg project
Authors: Picot Cyndie , Ajiji Priscilla , Jurek Lucie , Nourredine Mikail , Massardier Jérôme , Peron Audrey , Cucherat Michel , Cottin Judith 
Published: June 2023

Abstract
Background
Knowledge about the risks of drugs during pregnancy is continuously evolving due to the frequent publication of a large number of epidemiological studies. Systematic reviews and meta-analyses therefore need to be regularly updated to reflect these advances. To improve dissemination of this updated information, we developed an initiative of real-time full-scale living meta-analyses relying on an open online dissemination platform (www.metapreg.org).

Method
All living meta-analyses performed in this project will be conducted in accordance with this master protocol after adaptation of the search strategy. A systematic literature search of PubMed and Embase will be performed. All analytical studies (e.g., cohort, case-control, randomized studies) reporting original empirical findings on the association between in utero exposure to drugs and adverse pregnancy outcomes will be included. Study screening and data extraction will be performed in a semi-automation way supervised by a biocurator. A risk of bias will be assessed using the ROBINS-I tools. All clinically relevant pregnancy adverse outcomes (malformations, stillbirths, neuro-developmental disorders, pre-eclampsia, etc.) available in the included studies will be pooled through random-effects meta-analysis. Heterogeneity will be evaluated by I2 statistics.

Discussion
Our living systematic reviews and subsequent updates will inform the medical, regulatory, and health policy communities as the news results evolve to guide decisions on the proper use of drugs during the pregnancy.

Systematic review registration
Open Science Framework (OSF) registries
The impact of sports participation on mental health and social outcomes in adults: a systematic review and the ‘Mental Health through Sport’ conceptual model
Authors: Eather Narelle , Wade Levi , Pankowiak Aurélie , Eime Rochelle 
Published: June 2023

Abstract
Background
Sport is a subset of physical activity that can be particularly beneficial for short-and-long-term physical and mental health, and social outcomes in adults. This study presents the results of an updated systematic review of the mental health and social outcomes of community and elite-level sport participation for adults. The findings have informed the development of the ‘Mental Health through Sport’ conceptual model for adults.

Methods
Nine electronic databases were searched, with studies published between 2012 and March 2020 screened for inclusion. Eligible qualitative and quantitative studies reported on the relationship between sport participation and mental health and/or social outcomes in adult populations. Risk of bias (ROB) was determined using the Quality Assessment Tool (quantitative studies) or Critical Appraisal Skills Programme (qualitative studies).

Results
The search strategy located 8528 articles, of which, 29 involving adults 18–84 years were included for analysis. Data was extracted for demographics, methodology, and study outcomes, and results presented according to study design. The evidence indicates that participation in sport (community and elite) is related to better mental health, including improved psychological well-being (for example, higher self-esteem and life satisfaction) and lower psychological ill-being (for example, reduced levels of depression, anxiety, and stress), and improved social outcomes (for example, improved self-control, pro-social behavior, interpersonal communication, and fostering a sense of belonging). Overall, adults participating in team sport had more favorable health outcomes than those participating in individual sport, and those participating in sports more often generally report the greatest benefits; however, some evidence suggests that adults in elite sport may experience higher levels of psychological distress. Low ROB was observed for qualitative studies, but quantitative studies demonstrated inconsistencies in methodological quality.

Conclusions
The findings of this review confirm that participation in sport of any form (team or individual) is beneficial for improving mental health and social outcomes amongst adults. Team sports, however, may provide more potent and additional benefits for mental and social outcomes across adulthood. This review also provides preliminary evidence for the Mental Health through Sport model, though further experimental and longitudinal evidence is needed to establish the mechanisms responsible for sports effect on mental health and moderators of intervention effects. Additional qualitative work is also required to gain a better understanding of the relationship between specific elements of the sporting environment and mental health and social outcomes in adult participants.
Implementation determinants of HIV Self-Testing among young sexual minority men
Authors: Zapata Juan Pablo , Petroll Andrew E. , Quinn Katherine G. , Zamantakis Alithia , John Steven A. 
Published: June 2023

Abstract
Background
HIV self-testing (HIVST) has shown the potential for reaching people with heightened vulnerability to HIV, including young sexual minority men (YSMM), yet implementation of HIVST among YSMM aged 17–24 is scarce as a prevention method. Moreover, despite the consistent finding that offering HIVST increases HIV testing rates, barriers remain that need to be reduced in order to maximize the potential of this biomedical technology. Such information is necessary to direct implementation efforts to increase HIVST among YSMM, including HIV counseling and linkage to care. The current study was therefore intended to investigate perspectives for HIVST among YSMM and how HIVST can be marketed to increase implementation.

Methods
Between March and September 2020, we enrolled 41 YSMM to participate in one of nine online synchronous focus group discussions about their general experience with HIV preventive services. Guided by the Consolidated Framework (CFIR) for Implementation Research, we explored YSMM perspectives on facilitators and barriers to HIVST implementation. Data were analyzed using a deductive thematic content analysis approach.

Results
Many participants had never used HIVST before their participation in this study (n = 30; 73.2%). Qualitative results exhibited a variety of implementation determinants across the five CFIR 2.0 domains. Barriers included concerns about the format in which the testing materials would be provided (i.e., nature of packaging) and about the method in which the sample would need to be collected, particularly for those who had the testing kit mailed to their home address. These reservations were nested in the fear of unwanted disclosure of their sexual behavior, namely among the respondents who had to cohabitate with family due to the COVID-19 pandemic. Participants also discussed the limited local resources for HIVST. Many participants suggested programs that could be implemented to support HIVST, such as collaborations with trusted community agencies.

Conclusions
Understanding YSMM’ perspectives of HIVST may help identify implementation deficiencies within the delivery system and aid the development of implementation strategies to promote reach of HIVST.
Social acceptance of green hydrogen in Germany: building trust through responsible innovation
Authors: Häußermann Johann Jakob , Maier Moritz J. , Kirsch Thea C. , Kaiser Simone , Schraudner Martina 
Published: June 2023

Abstract
Background
Social acceptance presents a major challenge for Germany’s transition to green energy. As a power-to-x technology, green hydrogen is set to become a key component of a future sustainable energy system. With a view to averting conflicts like those surrounding wind energy, we have investigated social acceptance of green hydrogen at an early stage in its implementation, before wider rollout. Our study uses a mixed-method approach, wherein semi-structured interviews (n = 24) and two participatory workshops (n = 51) in a selected region in central Germany serve alongside a representative survey (n = 2054) as the basis for both understanding social attitudes and reaching generalisable conclusions.

Results
Overall, it is possible to observe both a marked lack of knowledge and a large degree of openness towards green hydrogen and its local use, along with high expectations regarding environmental and climate protection. We reach three key conclusions. First, acceptance of green hydrogen relies on trust in science, government, the media, and institutions that uphold distributive justice, with consideration for regional values playing a vital role in establishing said trust. Second, methodologically sound participatory processes can promote acceptance, and active support in particular. Third, recurrent positive participatory experiences can effectively foster trust.

Conclusions
Accordingly, we argue that trust should be strengthened on a structural level, and that green hydrogen acceptance should be understood as a matter of responsible innovation. As the first empirical investigation into social acceptance of green hydrogen, and by conceptually interlinking acceptance research and responsible innovation, this study constitutes an important contribution to existing research.
DHCR24 reverses Alzheimer’s disease-related pathology and cognitive impairment via increasing hippocampal cholesterol levels in 5xFAD mice
Authors: Zhang Wen-bin , Huang Yue , Guo Xiao-rou , Zhang Meng-qi , Yuan Xiang-shan , Zu Heng-bing 
Published: June 2023

AbstractAccumulating evidences reveal that cellular cholesterol deficiency could trigger the onset of Alzheimer’s disease (AD). As a key regulator, 24-dehydrocholesterol reductase (DHCR24) controls cellular cholesterol homeostasis, which was found to be downregulated in AD vulnerable regions and involved in AD-related pathological activities. However, DHCR24 as a potential therapeutic target for AD remains to be identified. In present study, we demonstrated the role of DHCR24 in AD by employing delivery of adeno-associated virus carrying DHCR24 gene into the hippocampus of 5xFAD mice. Here, we found that 5xFAD mice had lower levels of cholesterol and DHCR24 expression, and the cholesterol loss was alleviated by DHCR24 overexpression. Surprisingly, the cognitive impairment of 5xFAD mice was significantly reversed after DHCR24-based gene therapy. Moreover, we revealed that DHCR24 knock-in successfully prevented or reversed AD-related pathology in 5xFAD mice, including amyloid-β deposition, synaptic injuries, autophagy, reactive astrocytosis, microglial phagocytosis and apoptosis. In conclusion, our results firstly demonstrated that the potential value of DHCR24-mediated regulation of cellular cholesterol level as a promising treatment for AD.
Functional photoacoustic imaging: from nano- and micro- to macro-scale
Authors: Park Byullee , Oh Donghyeon , Kim Jeesu , Kim Chulhong 
Published: June 2023

AbstractFunctional photoacoustic imaging is a promising biological imaging technique that offers such unique benefits as scalable resolution and imaging depth, as well as the ability to provide functional information. At nanoscale, photoacoustic imaging has provided super-resolution images of the surface light absorption characteristics of materials and of single organelles in cells. At the microscopic and macroscopic scales. photoacoustic imaging techniques have precisely measured and quantified various physiological parameters, such as oxygen saturation, vessel morphology, blood flow, and the metabolic rate of oxygen, in both human and animal subjects. This comprehensive review provides an overview of functional photoacoustic imaging across multiple scales, from nano to macro, and highlights recent advances in technology developments and applications. Finally, the review surveys the future prospects of functional photoacoustic imaging in the biomedical field.
Investigating the implant position reproducibility of optical impressions obtained using an intraoral scanner and 3D-printed models fabricated using an intraoral scanner
Authors: Iwamoto Maya , Atsuta Wataru , Kaneko Yasuhide , Ito Junnosuke , Kanno Takeshi , Murakami Takahiro , Tanaka Jyoji 
Published: June 2023

Abstract
Purpose
This study aims to examine the effect of the size of the intraoral scanning area on implant position reproducibility and compare the implant position reproducibility of plaster models fabricated using the silicone impression technique, the digital model of an intraoral scanner, and three-dimensional (3D)-printed models fabricated using an intraoral scanner.

Methods
Scanbodies were attached to an edentulous model with six implants (master model) and were scanned using a dental laboratory scanner to obtain basic data. The plaster model was fabricated using the open-tray method (IMPM; n = 5). The master model was then scanned in various implant areas using an intraoral scanner to obtain data (IOSM; n = 5); the scanning data of six scanbodies were used to fabricate the 3D-printed models (3DPM; n = 5) using a 3D printer. Scanbodies were attached to the implant analogs of the IMPM and 3DPM models and data were obtained using a dental laboratory scanner. The basic data and IMPM, IOSM, and 3DPM data were superimposed to calculate the concordance rate of the scanbodies.

Results
The concordance rate of intraoral scanning decreased as the number of scanbodies increased. Significant differences were observed between the IMPM and IOSM data, and between the IOSM and 3DPM data; however, the IMPM and 3DPM data did not differ significantly.

Conclusions
The implant position reproducibility of the intraoral scanner decreased with an increase in the scanning area. However, ISOM and 3DPM may provide higher implant position reproducibility than plaster models fabricated using IMPM.
Patient’s assessment and prediction of recovery after stroke: a roadmap for clinicians
Authors: Salvalaggio Silvia , Boccuni Leonardo , Turolla Andrea 
Published: June 2023

Abstract
Background and purpose
In neurorehabilitation clinical practice, assessment is usually more oriented to evaluate patient’s present status, than to plan interventions according to predicted outcomes. Therefore, we conducted an extensive review of current prognostic models available in the literature for recovery prediction of many functions and constructs, after stroke. We reported results in the form of a practical guide for clinicians, with the aim of promoting the culture of early clinical assessment for patient stratification, according to expected outcome.

Summary of key points
To define a roadmap for clinicians, a stepwise sequence of five actions has been developed, from collecting information of past medical history to the adoption of validated prediction tools. Furthermore, a clinically-oriented organization of available prediction tools for recovery after stroke have been proposed for motor, language, physiological and independency functions. Finally, biomarkers and online resources with prognostic value have been reviewed, to give the most updated state of the art on prediction tools after stroke.

Recommendations for clinical practice
Clinical assessment should be directed both towards the objective evaluation of the present health status, and to the prediction of expected recovery. The use of specific outcome measures with predictive value is recommended to help clinicians with the definition of sound therapeutic goals.
Prevalence of psychological distress among the caregivers of an endosulfan disaster victims in India: a cross-sectional community-based study
Authors: Pillai Renjith R. , Kasi Sekar , Diwakar Dilip 
Published: June 2023

Abstract
Background
Physical health needs of disaster victims take priority over mental health needs in post-disaster scenario, assessments and interventions for latter needs are done only for a shorter duration. The caregivers of the victims also bore the brunt of the disaster and their mental health needs are seldom examined and addressed. Unable to find any literature examining the mental health needs of caregivers of the endosulfan disaster victims in India, the study was planned with the objectives of assessing the prevalence and predictors of psychological distress, its relation to the level of impact, disability as well as quality of life among the caregivers of the victims of endosulfan disaster in the State of Kerala, India after 20 years.

Method
It was a cross-sectional community-based study among 400 the caregivers of the victims of endosulfan disaster. Demographic details, distress, impact, disability and quality of life were measured using instruments such as Family Schedule, Self-Reporting Questionnaire, Impact of Events Scale, World Health Organization Disability Assessment Schedule 2.0 and World Health Organization Quality of Life Scale, respectively.

Results
The estimated psychological distress among the caregivers of the endosulfan disaster victims was 48.7%. Distressed caregivers were found to have significant higher impact (t = − 12.12, p < 0.001), higher disability (t = − 13.33, p < 0.001), and poorer quality of life (t = 11.64, p < 0.001) than non-distressed caregivers. On logistic regression analysis, the positive predictors of psychological distress among the caregivers of endosulfan victims were female sex, impact and psychological disability while quality of life was a negative predictor.

Conclusions
Almost half of the caregivers of the victims of endosulfan disaster victims reported psychological distress, high level of impact and disability and a poorer quality of life. It calls for periodical mental health needs assessment and a long-term sustainable community based psychosocial care and disaster mental health programme.
Sphenochoanal polyp in a child presented with recurrent middle ear effusion: a case report
Authors: Aljeraisi Talal 
Published: June 2023

Abstract
Background
Sphenochoanal is a rare benign sinu-nasal lesion. It arises from the sphenoid sinus and passes to the choana then to the nasopharynx. It usually presents with nasal obstruction, discharge, snoring, and headache. It has been reported—only once in the English literature—to cause Eustachian tube dysfunction and middle ear effusion (MEE) in an adult male. In this report, we described an 8-year-old child with a sphenochoanal polyp presented with MEE.

Case presentation
An 8-year-old boy was referred to us by his family physician with recurrent earache. Otoscopic examination showed MEE on the left side. Anterior rhinoscopy showed a thick grayish discharge in the left nasal cavity. Nasal endoscopic examination showed a polyp in the posterior part of the left nasal cavity obstructing the choana. Computed tomography revealed soft tissue density on the left sphenoid sinus extended to the choana and nasopharynx. Diagnosis of sphenochoanal polyp was established. Endoscopic sinus surgery with removal of the lesion and ventilation tube insertion in the left ear was performed. Histopathologic examination confirmed that the polyp was inflammatory. Follow-up for 1 year showed no recurrence.

Conclusions
Sphenochoanal which is a rare lesion in children could be a cause of Eustachian tube dysfunction and MEE. Nasal endoscopy and computed tomography are important for diagnosis. We recommend to list sphenochoanal polyp as a cause of Eustachian tube dysfunction and recurrent MEE in children.