bluuebunny/crossref_metadata_2025_split huggingface dataset:
In search of lost time: tracing the fossil diversity of Podocarpaceae through the ages
Authors: Andruchow-Colombo Ana , Escapa Ignacio H , Aagesen Lone , Matsunaga Kelly K S 
Published: August 2023

AbstractThe Podocarpaceae are a morphologically diverse conifer family that have a cryptic fossil record reported since the Permian. We reviewed the fossil record of Podocarpaceae, tested the affinities of its oldest records using phylogenetic analyses, compiled macrofossil occurrence records, and investigated the diversity, distribution, and morphology of Podocarpaceae through time. We found that Permian, Triassic, and some Jurassic fossils referred to Podocarpaceae should not be placed in the family. Our total-evidence phylogenetic analyses, which sampled all major conifer lineages, recovered the Triassic Rissikia and the Jurassic Nothodacrium as stem-group conifers and the Jurassic Mataia as part of the Araucariales stem group. We further discuss the phylogenetic position of the Mesozoic enigmatic conifers Pararaucaria (Cheirolepidiaceae) and Telemachus (Voltziales), which were recovered most frequently in the conifer stem group. We conclude that the earliest reliable Podocarpaceae occurrences are from the Jurassic of both hemispheres and have scale-like leaves. Most extant genera appear in the fossil record between the Late Cretaceous and the Early Cenozoic. Many extant leaf morphologies appear in the Early Cretaceous, coeval with angiosperm diversification, consistent with the hypothesis that expanded leaves in Podocarpaceae are adaptive responses for light harvesting in angiosperm-dominated environments today.
“Rest as resistance:” Black cyberfeminism, collective healing and liberation on @TheNapMinistry
Authors: Monier Mel 
Published: August 2023

Abstract
Tricia Hersey, founder of The Nap Ministry, uses rest as a “healing mechanism” and a form of resistance. With a vibrant social media following, the Nap Ministry builds upon a strong history of Black feminist activism that centers the lived experiences of Black women. According to Hersey, rest is personal and political, a fight against the commodification and exploitation of laboring Black folks from chattel slavery to contemporary grind and hustle culture. This article explores how Hersey builds upon a lineage of Black women’s activism in digital spaces while also exploring rest, “soul care,” and communal healing as ideologies rooted in Black (cyber)feminism. I argue that Nap Ministry constellates Womanism, Black (cyber)feminism, and Black liberation theory by promoting the power of rest, encouraging resistance against what bell hooks describes as the “imperialist white supremacist capitalist patriarchy” that exploits Black women’s labor and renders their voices invisible.
Long-Read Sequencing with Hierarchical Clustering for Antiretroviral Resistance Profiling of Mixed Human Immunodeficiency Virus Quasispecies
Authors: Ng Timothy Ting-Leung , Su Junhao , Lao Hiu-Yin , Lui Wui-Wang , Chan Chloe Toi-Mei , Leung Amy Wing-Sze , Jim Stephanie Hoi-Ching , Lee Lam-Kwong , Shehzad Sheeba , Tam Kingsley King-Gee , Leung Kenneth Siu-Sing , Tang Forrest , Yam Wing-Cheong , Luo Ruibang , Siu Gilman Kit-Hang 
Published: August 2023

Abstract

Background
HIV infections often develop drug resistance mutations (DRMs), which can increase the risk of virological failure. However, it has been difficult to determine if minor mutations occur in the same genome or in different virions using Sanger sequencing and short-read sequencing methods. Oxford Nanopore Technologies (ONT) sequencing may improve antiretroviral resistance profiling by allowing for long-read clustering.

Methods
A new ONT sequencing-based method for profiling DRMs in HIV quasispecies was developed and validated. The method used hierarchical clustering of long amplicons that cover regions associated with different types of antiretroviral drugs. A gradient series of an HIV plasmid and 2 plasma samples was prepared to validate the clustering performance. The ONT results were compared to those obtained with Sanger sequencing and Illumina sequencing in 77 HIV-positive plasma samples to evaluate the diagnostic performance.

Results
In the validation study, the abundance of detected quasispecies was concordant with the predicted result with the R2 of &gt; 0.99. During the diagnostic evaluation, 59/77 samples were successfully sequenced for DRMs. Among 18 failed samples, 17 were below the limit of detection of 303.9 copies/μL. Based on the receiver operating characteristic analysis, the ONT workflow achieved an F1 score of 0.96 with a cutoff of 0.4 variant allele frequency. Four cases were found to have quasispecies with DRMs, in which 2 harbored quasispecies with more than one class of DRMs. Treatment modifications were recommended for these cases.

Conclusions
Long-read sequencing coupled with hierarchical clustering could differentiate the quasispecies resistance profiles in HIV-infected samples, providing a clearer picture for medical care.
Effect of intraoperative botulinum toxin injection on delayed gastric emptying and need for endoscopic pyloric intervention following esophagectomy: a systematic review, meta-analysis, and meta-regression analysis
Authors: Hajibandeh Shahab , Hajibandeh Shahin , McKenna Matthew , Jones William , Healy Paul , Witherspoon Jolene , Blackshaw Guy , Lewis Wyn , Foliaki Antonio , Abdelrahman Tarig 
Published: August 2023

Abstract
The aim of this study was to evaluate the effect of intraoperative botulinum toxin (BT) injection on delayed gastric emptying (DGE) and need for endoscopic pyloric intervention (NEPI) following esophagectomy. In compliance with Preferred Reporting Items for Systematic reviews and Meta-Analyses statement standards, a systematic review of studies reporting the outcomes of intraoperative BT injection in patients undergoing esophagectomy for esophageal cancer was conducted. Proportion meta-analysis model was constructed to quantify the risk of the outcomes and direct comparison meta-analysis model was constructed to compare the outcomes between BT injection and no BT injection or surgical pyloroplasty. Meta-regression was modeled to evaluate the effect of variations in different covariates among the individual studies on overall summary proportions. Nine studies enrolling 1070 patients were included. Pooled analyses showed that the risks of DGE and NEPI following intraoperative BT injection were 13.3% (95% confidence interval [CI]: 7.9–18.6%) and 15.2% (95% CI: 7.9–22.5%), respectively. There was no difference between BT injection and no BT injection in terms of DGE (odds ratio [OR]: 0.57, 95% CI: 0.20–1.61, P = 0.29) and NEPI (OR: 1.73, 95% CI: 0.42–7.12, P = 0.45). Moreover, BT injection was comparable to pyloroplasty in terms of DGE (OR: 0.85, 95% CI: 0.35–2.08, P = 0.73) and NEPI (OR: 8.20, 95% CI: 0.63–105.90, P = 0.11). Meta-regression suggested that male gender was negatively associated with the risk of DGE (coefficient: −0.007, P = 0.003). In conclusion, level 2 evidence suggests that intraoperative BT injection may not improve the risk of DGE and NEPI in patients undergoing esophagectomy. The risk of DGE seems to be higher in females and in early postoperative period. High quality randomized controlled trials with robust statistical power are required for definite conclusions. The results of the current study can be used for hypothesis synthesis and power analysis in future prospective trials.
Peroral endoscopic myotomy compared to laparoscopic Heller myotomy and pneumatic dilation in the treatment of achalasia: a systematic review
Authors: North Adam , Tewari Nilanjana 
Published: August 2023

Abstract
Peroral endoscopic myotomy (POEM) is an intervention for the treatment of achalasia which has gained popularity over the last decade. It’s efficacy and invasiveness are comparable to laparoscopic Heller myotomy (LHM). The purpose of this systematic review is to compare POEM to existing therapies. The systematic review was performed following the preferred reporting items for systematic reviews and meta-analyses (PRISMA) guidelines. MEDLINE, EMBASE, Web of Science and Cochrane Libraries were searched using keywords: esophageal achalasia, POEM, LHM, pneumatic dilation (PD), and related terms. The studied outcomes were Eckardt score, clinical success, hospital stay, cost-utility analysis, complications, and post-treatment gastro-esophageal reflux disease. Articles were reviewed by one researcher and uncertainty was resolved by a second researcher. The search strategy retrieved 1948 citations. After removing duplicates and applying the exclusion criteria, 91 studies were selected for full-text review of which a total of 31 studies were considered eligible for further analysis, including two studies which were found through manual searching. POEM has improved efficacy compared to PD with similar cost-effectiveness. POEM results showed comparable patient outcomes when compared with laparoscopic myotomy. Overall, POEM is a feasible first-line treatment for achalasia.
The (Non-)Use of African Law by the International Criminal Court
Authors: Manley Stewart , Tehrani Pardis Moslemzadeh , Rasiah Rajah 
Published: August 2023

Abstract
All defendants before the International Criminal Court (ICC) to date have been African, with their alleged crimes having been committed, at least partly, on African soil. When turning to national laws to resolve issues of interpretation in these cases, should the ICC see whether it can use laws of the African state in which the crime occurred? This article argues that it should, but observes – from a dataset of 16,192 citations containing over 200 citations to national laws – that it rarely does. Instead, it turns much more often to Western European and US laws. This phenomenon, the article suggests, troublingly reflects and perpetuates the marginalization of African and other global South laws from what constitutes international law. The article also argues that the Rome Statute requires the ICC to at least examine for appropriateness the laws of a subset of these neglected systems (‘the national laws of States that would normally exercise jurisdiction over the crime’) when identifying general principles of law. There are several compelling reasons to extend this examination requirement to African and other global South country laws more broadly, and even when not dealing with general principles of law, and few reasons not to.
Gender representation amongst orthodontic editorial boards: trends over time
Authors: Reeves Samuel , Liu Catherine , Mack Gavin 
Published: August 2023

Summary

Objective
To assess trends in gender representation within editorial boards of European and North American orthodontic journals from 2000 to 2023.

Materials and methods
Retrospective cross-sectional study of editorial boards of orthodontic journals. The first issue of volumes of identified journals from the years 2000, 2010 and 2023 were investigated to demonstrate trends over time.

Results
Eight journals were included. Across the period investigated, a total of 810 editorial board positions were identified with most positions held by men (657/810; 81.1%). The proportion of female editorial board members increased from 14.7% (36/245) in 2000 to 24.5% (73/298) in 2023. The number of female Editor-in-Chief increased from zero in 2000 to two in 2023. Three journals demonstrated a reduction in female representation from 2000 to 2023.

Conclusion
Females remain underrepresented within editorial boards of orthodontic journals which is a key metric of presence and leadership in orthodontic academia.
On the trail of the Willmott and Braikenridge manuscripts
Authors: Burke James 
Published: August 2023

Abstract
The Willmott and Braikenridge manuscripts, once owned by the Norwich merchant John Sadler (d.1592), are all that is known to survive from his second partbook set, dated 1591. This article explores the custodial history of those volumes, tracing their various later owners and the marks they left. It finds that the two surviving volumes may have been apart since 1709—a separation that persisted until they were reunited in 2022—and also that the set’s three missing partbooks may have been lost only since 1885, raising hopes that they may one day be found.
A GFP splicing reporter in a coilin mutant background reveals links between alternative splicing, siRNAs, and coilin function in Arabidopsis thaliana
Authors: Kanno Tatsuo , Chiou Phebe , Wu Ming-Tsung , Lin Wen-Dar , Matzke Antonius , Matzke Marjori 
Published: August 2023

Abstract
Coilin is a scaffold protein essential for the structure of Cajal bodies, which are nucleolar-associated, nonmembranous organelles that coordinate the assembly of nuclear ribonucleoproteins (RNPs) including spliceosomal snRNPs. To study coilin function in plants, we conducted a genetic suppressor screen using a coilin (coi1) mutant in Arabidopsis thaliana and performed an immunoprecipitation-mass spectrometry analysis on coilin protein. The coi1 mutations modify alternative splicing of a GFP reporter gene, resulting in a hyper-GFP phenotype in young coi1 seedlings relative to the intermediate wild-type level. As shown here, this hyper-GFP phenotype is extinguished in older coi1 seedlings by posttranscriptional gene silencing triggered by siRNAs derived from aberrant splice variants of GFP pre-mRNA. In the coi1 suppressor screen, we identified suppressor mutations in WRAP53, a putative coilin–interacting protein; SMU2, a predicted splicing factor; and ZCH1, an incompletely characterized zinc finger protein. These suppressor mutations return the hyper-GFP fluorescence of young coi1 seedlings to the intermediate wild-type level. Additionally, coi1 zch1 mutants display more extensive GFP silencing and elevated levels of GFP siRNAs, suggesting the involvement of wild-type ZCH1 in siRNA biogenesis or stability. The immunoprecipitation-mass spectrometry analysis reinforced the roles of coilin in pre-mRNA splicing, nucleolar chromatin structure, and rRNA processing. The participation of coilin in these processes, at least some of which incorporate small RNAs, supports the hypothesis that coilin provides a chaperone for small RNA trafficking. Our study demonstrates the usefulness of the GFP splicing reporter for investigating alternative splicing, ribosome biogenesis, and siRNA-mediated silencing in the context of coilin function.
Histological Disease Activity as Predictor of Clinical Relapse, Hospitalization, and Surgery in Inflammatory Bowel Disease: Systematic Review and Meta-Analysis
Authors: Shehab Mohammad , Al Akram Sahad , Hassan Amro , Alrashed Fatema , Jairath Vipul , Bessissow Talat 
Published: August 2023

Abstract

Background
The clinical impact of histological remission on short- and long-term clinical outcomes in patients with inflammatory bowel disease (IBD) is not well established. We assessed risk of clinical relapse, hospitalization, and need for surgery in patients achieving histological remission in comparison with active histological disease.

Methods
A systematic review was conducted using MEDLINE, Scopus, Cochrane CENTRAL, EMBASE, and conference abstracts from inception to November 2022. Our main outcome was the rate of clinical relapse in patients with IBD who reached histological remission vs patients with active histological disease. Secondary outcomes were clinical complications of IBD such as hospitalization and need for surgery. The endpoints were investigated at 2 time points, 6 to 12 months (short term) and &gt;12 months (long term).

Results
Short-term outcome analysis showed that the risk of clinical relapse was significantly higher in ulcerative colitis patients with active histological disease in comparison with patients at histological remission (risk ratio [RR], 2.41; 95% confidence interval [CI], 1.69-3.44; P &lt; .01). The risk of hospitalization in ulcerative colitis patients was not significant among the 2 groups (RR, 4.22; 95% CI, 0.91-19.62; P = .07). Long-term outcome analysis demonstrated that the risk of clinical relapse (RR, 2.07; 95% CI, 1.55-2.76; P &lt; .01), need for surgery (RR, 3.14; 95% CI, 1.53-6.45; P &lt; .01), and hospitalization (RR, 2.52; 95% CI, 1.59-4.00; P &lt; .01) was significantly higher in patients with active histological disease.

Conclusions
Histological remission in IBD represents an important therapeutic goal that is not yet routinely pursued in clinical practice. In our study, patients who achieved histological remission have more favorable outcomes than those with active histological disease in ulcerative colitis.
Investigation of 3-(1,3-oxazol-5-yl)aniline as a highly efficient corrosion inhibitor for mild steel in 1 M HCl solution
Authors: Alamiery Ahmed , Al-Azzawi Waleed K 
Published: August 2023

AbstractMild steel corrosion in acidic environments presents a significant challenge with detrimental consequences for both industrial infrastructure and the environment. Effective corrosion control measures are crucial to prolonging the lifespan of equipment. Inhibition techniques have proven to be an efficient method for protecting mild steel from corrosion, particularly in acidic conditions. This study investigates the efficacy of 3-(1,3-oxazol-5-yl)aniline (3-OYA) as a corrosion inhibitor for mild steel in a hydrochloric acid (HCl) solution. Traditional weight-loss tests, as well as electrochemical techniques, such as potentiodynamic polarization and electrochemical impedance spectroscopy, were employed to evaluate the corrosion inhibition performance. The results reveal that 3-OYA, at a concentration of 0.05 mM, exhibits an outstanding protection efficacy of 93.5%. This remarkable performance can be attributed to the formation of a protective adsorption layer on the mild steel surface, effectively inhibiting the corrosion rate and enhancing inhibitory efficacy. The inhibition efficiency was found to increase with increasing inhibitor concentration, while it decreased with rising temperature. Langmuir adsorption isotherm analysis confirmed the high adsorption–inhibition activity of 3-OYA. The $\Delta {G}_{ads}^o$ value indicated the occurrence of both physical and chemical adsorption mechanisms on the mild steel surface. Furthermore, density functional theory (DFT) calculations were utilized to determine the quantum chemical parameters and establish a correlation between the inhibition activity and the molecular structure. The consistency between the experimental and theoretical analyses reinforces the robustness of our findings.
Subordination Theory in Practice: An Empirical Analysis of Chinese Courts’ Approaches to Classifying Labour Relationships in Platform Cases
Authors: Zheng Qi , Su Jianning 
Published: August 2023

Abstract
Determining the juridical nature of the work relationship is key to understanding the capacity of labour law to protect the rights and interests of gig workers. How to apply the traditional ‘subordination’ test and its constituent elements to platform work remains controversial in both theory and practice. In this article we examine the approaches of courts to this question using a sample of 71 Chinese judgments. We construct a binary probit model to conduct an empirical test of the degree of correlation between eight factors which serve to identify protected worker status, on the one hand, and courts’ rulings on the presence or absence of subordination, on the other. We find that, among the factors relevant to the subordination test, ‘control’ and ‘integration’ strongly predict a finding of worker status. We conclude that the subordination test is still being widely used to determine the status of gig workers in China, and that an approach to classification based on the ‘primacy of the facts’ of the case continues to be feasible in the context of platform work.
A Call to Action: Current Challenges and Considerations for COVID-19 Vaccination in Immunocompromised Populations
Authors: Bonanni Paolo , Ceddia Francesca , Dawson Rachel 
Published: August 2023

Abstract
The heightened risk of infection and complexities of preventing disease in immunocompromised individuals are at the forefront of public health strategies. The COVID-19 pandemic highlighted the increased vulnerability and susceptibility to serious outcomes in this population. COVID-19 prevention efforts led to the development of vaccines, including mRNA-based options, which were initially recommended as a 2-dose primary schedule for both immunocompromised and immunocompetent individuals. However, post-rollout assessments led to updated recommendations specific to immunocompromised populations. As COVID-19 potentially transitions to become endemic disease, immunocompromised individuals will remain at high risk of severe disease; thus, the evaluation of current vaccination challenges remains crucial for guiding effective public health efforts. This article summarizes key findings from the previous articles of this supplement, highlighting current vaccination challenges for at-risk immunocompromised groups and exploring solutions to ensure protection against COVID-19 for these vulnerable populations.
Omega-3 meat enrichment and L-FABP, PPARA, and LPL genes expression are modified by the level and period of tuna oil supplementation in slow-growing chickens
Authors: Khosinklang Wichuta , Kubota Satoshi , Riou Cindy , Kaewsatuan Pramin , Molee Amonrat , Molee Wittawat 
Published: August 2023

Abstract
This study proposes a strategy to manipulate the fatty acid (FA) content in slow-growing Korat chicken (KRC) meat using tuna oil (TO). To determine the optimal level and feeding period of TO supplementation, we conducted a study investigating the effects of dietary TO levels and feeding periods on meat quality, omega-3 polyunsaturated fatty acid (n-3 PUFA) composition, and gene expression related to FA metabolism in KRC breast meat. At 3 wk of age, 700 mixed-sex KRC were assigned to seven augmented factorial treatments with a completely randomized design, each consisting of four replicate pens containing 25 chickens per pen. The control group received a corn-soybean-based diet with 4.5% rice bran oil (RBO), while varying amounts of TO (1.5%, 3.0%, or 4.5%) replaced a portion of the RBO content in the experimental diets. The chickens were fed these diets for 3 and 6 wk, respectively, before being slaughtered at 9 wk. Our results indicated no significant interactions between TO levels and feeding periods on the growth performance or meat quality of KRC (P &gt; 0.05). However, the liver fatty acid-binding protein gene (L-FABP, also known as FABP1), responsible for FA transport and accumulation, showed significantly higher expression in the chickens supplemented with 4.5% TO (P &lt; 0.05). The chickens supplemented with 4.5% TO for a longer period (3 to 9 wk of age) exhibited the lowest levels of n-6 PUFA and n-6 to n-3 ratio, along with the highest levels of eicosapentaenoic acid, docosahexaenoic acid, and n-3 PUFA in the breast meat (P &lt; 0.05). However, even a short period of supplementation with 4.5% TO (6 to 9 wk of age) was adequate to enrich slow-growing chicken meat with high levels of n-3 PUFA, as recommended previously.
Our findings indicated that even a short period of tuna oil supplementation could lead to desirable levels of omega-3 enrichment in slow-growing chicken meat. This finding has practical implications for the poultry industry, providing insights into optimal supplementation strategies for achieving desired FA profiles without adversely affecting growth performance or meat quality.