bluuebunny/crossref_metadata_2025_split huggingface dataset:
Computational resolution in single molecule localization – impact of noise level and emitter density
Authors: Hockmann Mathias , Kunis Stefan , Kurre Rainer 
Published: February 2023

Abstract
Classical fluorescence microscopy is a powerful technique to image biological specimen under close-to-native conditions, but light diffraction limits its optical resolution to 200–300 nm-two orders of magnitude worse than the size of biomolecules. Assuming single fluorescent emitters, the final image of the optical system can be described by a convolution with the point spread function (PSF) smearing out details below the size of the PSF. In mathematical terms, fluorescence microscopy produces bandlimited space-continuous images that can be recovered from their spatial samples under the conditions of the classical Shannon-Nyquist theorem. During the past two decades, several single molecule localization techniques have been established and these allow for the determination of molecular positions with sub-pixel accuracy. Without noise, single emitter positions can be recovered precisely – no matter how close they are. We review recent work on the computational resolution limit with a sharp phase transition between two scenarios: 1) where emitters are well-separated with respect to the bandlimit and can be recovered up to the noise level and 2) closely distributed emitters which results in a strong noise amplification in the worst case. We close by discussing additional pitfalls using single molecule localization techniques based on structured illumination.
Elektrische Levitationsdetektion für die Verwendung im Tissue Engineering
Authors: Scheunemann Stefan , Pientschke Christoph , Zabarylo Urszula Joanna , Raum Kay 
Published: February 2023

Zusammenfassung
Die Züchtung und Gestaltung biologischen Gewebes im Labor (Tissue Engineering) verspricht breite medizinische Anwendung. Die notwendige Strukturierung der künstlichen Gewebe kann mittels stehender Ultraschallwellen durch Levitation erfolgen. In diesem Beitrag wird eine Methode zur elektrischen Detektion der Levitation und Experimente zu ihrer Verifizierung vorgestellt. Die Ultraschallwellen werden mit piezoelektrischen Wandlern erzeugt. Die Kontrolle der Levitation beruht auf der Auswertung der Sendespannung. Optische Aufnahmen levitierter Kunststoffpartikel erlaubten die frequenzabhängige Ermittlung der Levitationsebenenabstände und eine Korrelation zu den Sendespannungen. Es zeigte sich, dass die Levitationsbedingung über einen relativ breiten Frequenzbereich erfüllt ist. Durch Berücksichtigung der frequenzabhängigen Sendeleistung kann mittels einer normierten Kurve ein gleichmäßiger Schalldruck über den gesamten für die Levitation relevanten Frequenzbereich eingestellt werden. Die gewonnenen Ergebnisse fließen in die Entwicklung eines Gerätes ein, mit dem parallel mehrere Proben für das Tissue Engineering per Levitation gezielt strukturiert werden können. Die Proben werden dabei nutzerfreundlich in Silikon- oder Teflon-Kammern prozessiert, indem ein Ultraschallsender und eine Reflektorplatte verwendet werden. Damit können Versuchsreihen zum Tissue-Engineering effizienter als bisher durchgeführt werden.
AKSI SOSIAL GERAKAN UNTUK KESEJAHTERAAN TUNARUNGU INDONESIA JAKARTA (GERKATIN JAKARTA) TERHADAP PENYANDANG DISABILITAS RUNGU
Authors: Sarah Agusti Siti 
Published: February 2023

This study aims to determine participation and benefits in the management of the Serasi Garbage Bank with the formulation of the problem: (1) How does public participation in the management of the Serasi Garbage Bank in Dago Benda Baru Pamulang South Tangerang (2) What are the benefits of the Serasi Garbage Bank in the Dago Benda Baru Pamulang Tangerang area South. The research methodology conducted by researchers is research conducted through a descriptive qualitative approach. Qualitative research is research that produces analytical procedures that do not use statistical analysis procedures or other quantitative methods, namely data obtained in the form of words, images and behavior. Through the Serasi Garbage Bank program, is a program that establishes and invites the community to manage household waste properly, the Serasi Garbage Bank program is an individual and collective waste management system with the principle of recycling cooperation with business partners.The benefits of managing the Serasi Garbage Bank, on the social aspects of the community, are concerned about waste and the environment. Economic aspects are still relatively small or a little income from weighing but can be an added value for the community. This aspect of the environment becomes cleaner, more beautiful and safer.
PEMBERDAYAAN MASYARAKAT DALAM PERKUMPULAN PENCAK SILAT CINGKRIG SERBAGUNA MELALUI PROGRAM PELATIHAN PENCAK SILAT
Authors: Hidayatullah Taufik 
Published: February 2023

Silat Cingkrig merupakan faktor penyatu dan pengikat dari masyarakat Betawi. Hal ini bisa dibuktikan bahwa silat cingkrig banyak digunakan dalam adat Betawi pada acara- acara, seperti pernikahan yaitu palang pintu. Namun seiring berjalannya waktu, kemajuan teknologi baik dalam bidang teknologi informasi maupun teknologi transportasi mendorong munculnya produk-produk kebudayaan baru dalam masyarakat yang telah membuat tradisi kebudayaan Betawi kian jarang terlihat. Akhirnya sebagian generasi muda kurang mengetahui tradisi kesenian Betawi, salah satunya Silat Cingkrig dan tradisi buka palang pintu pada perkawinan masyarakat Betawi. Keberadaan sebuah “Perkumpulan Pencak Silat Cingkrig Serbaguna” yang didirikan oleh H. Sinan memberikan dampak berupa perubahan ekosistem lingkungan hidup dan perubahan perilaku sosial dan budaya. Pendekatan pemberdayaan masyarakat menjadi salah satu cara dalam pengembangan masyarakat mandiri. Salah satunya adalah melalui pembentukan perkumpulan tersebut yang kemudian dilatih agar mempunyai kemauan, pengetahuan, dan kemampuan untuk mandiri. Pemberdayaan merupakan hakikat pendidikan karena apa yang disebut dengan pendidikan adalah usaha untuk memberdayakan individu, meningkatkan kemampuan individu, dan mengembangkan potensi yang ada pada diri individu tersebut. Penelitian ini dilakukan dengan menggunakan jenis penelitian kualitatif yang bersifat deskriptif dengan teknik pengumpulan data yang digunakan yaitu observasi, wawancara, dan studi dokumentasi. Adapun teori yang digunakan dalam penelitian ini yaitu teori tahapan pemberdayaan yang dikemukakan oleh Muhtadi dan Hermansyah yang menjelaskan tahapan POAC (Planning, Organizing, Actuating, dan Controlling). Hasil dari penelitian ini menunjukan bahwa dengan adanya Perkumpulan Pencak Silat Cangkring Serbaguna tersebut dengan menggunakan proses pemberdayaan yang dilakukan menghasilkan dua hasil yaitu, dapat meningkatkan pelestarian budaya Betawi dan meningkatkan kesejahteraan para aggotanya untuk dapat mendapatkan penghasilan.
Diastolic dysfunction in late postmenopausal patients with undifferentiated connective tissue disease and hypertension
Authors: Shambatov A. M. , Izmozherova N. V. , Popov A. A. , Grishina I. F. 
Published: February 2023

Aim. To assess myocardial diastolic function (DF) in late postmenopausal women with undifferentiated connective tissue disease (UCTD) and hypertension (HTN).Material and methods. This cross-sectional study included 135 postmenopausal women, the median age of which was 68 years (65÷70,5 years). The anamnesis was collected using a standardized questionnaire. Verification of UCTD was carried out according to clinical guidelines. All patients underwent standard transthoracic echocardiography. The assessment of left ventricular (LV) DF was carried out according to the transmitral flow. LV diastolic dysfunction (DD) was classified into three types: rigid, pseudonormal, and restrictive. Statistical processing was carried out in the STATISTICA 13.0 environment. The measure of data averaging is the median, the measure of dispersion is 25%÷75%. The significance of differences was assessed using the Mann-Whitney test. Differences were considered significant at p˂0,05.Results. Group 1 — 20 (14,8%) patients with verified UCTD and HTN, group 2 — 88 (65,2%) patients with HTN without UCTD, control group — 23 (30%) patients without HTN and UCTD. There were no differences in age, duration of postmenopause and body mass index between the groups. In the first group, a significant decrease in the ratio of peak early to late diastolic LV filling velocity was revealed (p˂0,01). A significant increase in left ventricular end-systolic wall stress revealed in group 1. In 108 (100%) patients, LVDD was detected; among the patients of the control group, DD was not detected. In 8 (40%) patients in group 1, a pseudo-normal type of DD was detected, while in 12 out of 20 patients (60%) — rigid type of DD. When assessing DF in patients of group 2, a significant decrease was found in the ratio of peak early to late diastolic LV filling velocity, a significant increase in LV end-diastolic wall stress and end-diastolic pressure. In 2 out of 3 (57,80%) patients of group 2, DD of the rigid type was detected, while pseudonormal type — in 32,2% of patients in this group. Group 2 patients had a significant decrease in the early diastolic mitral annular velocity (p˂0,01).Conclusion. The analysis of myocardial echocardiographic characteristics indicates a significant contribution of HTN-associated UCTD to the development of LVDD in postmenopausal women.
Changes in aortic root dimensions after ascending aortic repair with concomitant aortic valve replacement
Authors: Panfilov D. S. , Bazarbekova B. A. , Panfilova N. O. , Saushkin V. V. , Sonduev E. L. , Sazonova S. I. , Kozlov B. N. 
Published: February 2023

The issue of ascending aortic repair with concomitant aortic valve replacement in pa-tients with ascending aortic aneurysm (AscAA) and aortic valve stenosis is still debatable.Aim. To analyze the dimension changes of the preserved aortic root after simultaneous ascending aorta repair and aortic valve replacement.Material and methods. This retrospective study included 102 patients who, from December 2012 to May 2022, underwent simultaneous aortic valve replacement and ascending aorta repair with hemiarch replacement. Patients were divided into 2 following groups based on the aortic valve morphology: group 1 — patients with bicuspid aortic valve (BAV) and AscAA (n=75), group 2 — patients with tricuspid aortic valve (TAV) and AscAA (n=27). Depending on the presence of aortic root dilatation (maximum diameter (d) >40 mm), each of the groups was additionally stratified into 2 more subgroups as follows: patients without aortic root dilatation (d≤40 mm) and patients with its dilatation (d>40 mm). The dynamics of the aortic root diameter was assessed by computed tomography angiography.Results. The mean follow-up period for patients was 36,2±14,6 months. Survival rate in the BAV+AscAA and TAV+AscAA groups was 96% and 100%, respectively (p=0,380). Freedom from aortic root resurgery was 100% in both study groups. In patients with dilated and non-dilated aortic root of the BAV+AscAA group, an increase in aortic root dimension was noted at a rate of 0,65±0,51 mm/year and 0,32±0,27 mm/year, respectively. In patients of the TAV+AscAA group, a regression in dilated and non-dilated aortic root diameter was observed as follows: 0,93±0,48 mm/year and 0,56±0,43 mm/year, respectively.Conclusion. In patients with AscAA in combination with BAV stenosis after a singlestep surgical intervention, a weak negative dynamics of non-dilated and dilated aortic root is observed in the mid-term follow-up period. In patients with AscAA and TAV, there is involutive alterations of the aortic root dimension during 3-year follow-up.
Dynamics of global and segmental strain as a marker of right ventricular contractility recovery in patients after COVID-19 pneumonia
Authors: Shirokov N. E. , Yaroslavskaya E. I. , Krinochkin D. V. , Osokina N. A. 
Published: February 2023

Aim. To study the changes of morphological and functional right ventricular (RV) parameters depending on the severity of coronavirus infection 2019 (COVID-19) pneumonia over long-term follow-up.Material and methods. A total of 200 patients (men, 51,5%, mean age, 51,4±10,9 years) were examined at 2 control visits (3, 12 months after receiving two negative polymerase chain reaction tests). Patients were divided into following groups: group I (n=94) — lung tissue involvement ≥50% according to inhospital chest computed tomography (chest CT), group II (n=106) — lung tissue involvement˂50% according to chest CT.Results. The groups were comparable in key clinical and functional parameters 3 months after COVID-19 pneumonia. Speckle tracking echocardiography (STE) revealed a significant increase in following global longitudinal strain (LS) parameters: RV free wall endocardial LS (-22,7±3,2% and -24,3±3,8% in group I, p<0,001; -23,2±3,5% and -24,5±3,4% in group II, p><0,001), and RV endocardial LS (-21,0±3,1% and -22,5±3,7% in group I, p><0,001, -21,5±3,2% and -22,6±3,3% in group II, p=0,001 ). Significant increase of segmental endocardial LS was revealed in group I in the basal segments of RV free wall (-26,2±5,1% and -28,1±5,1%, p=0,004) and interventricular septum (IVS) (-16,2 [13,9; 19,5]% and -17,5 [14,6; 21,4]%, p=0,024), IVS middle segment (-20,3±4,1% and -21,5±4,8%, p=0,030), as well as in group II in the apical segments of RV free wall (-21,9±6,7% and -24,4±5,2%, p=0,001) and IVS (-23,7±4,7% and -24,9±4,8%, p=0,014). Conclusion. Recovery of RV function during a 12-month follow-up period in patients with both severe and moderate/mild lung involvement in COVID-19 was detected using the STE method.>˂0,001; -23,2±3,5% and -24,5±3,4% in group II, p˂0,001), and RV endocardial LS (-21,0±3,1% and -22,5±3,7% in group I, p˂0,001, -21,5±3,2% and -22,6±3,3% in group II, p=0,001 ). Significant increase of segmental endocardial LS was revealed in group I in the basal segments of RV free wall (-26,2±5,1% and -28,1±5,1%, p=0,004) and interventricular septum (IVS) (-16,2 [13,9; 19,5]% and -17,5 [14,6; 21,4]%, p=0,024), IVS middle segment (-20,3±4,1% and -21,5±4,8%, p=0,030), as well as in group II in the apical segments of RV free wall (-21,9±6,7% and -24,4±5,2%, p=0,001) and IVS (-23,7±4,7% and -24,9±4,8%, p=0,014).Conclusion. Recovery of RV function during a 12-month follow-up period in patients with both severe and moderate/mild lung involvement in COVID-19 was detected using the STE method.
Comparison of the novel START vascular stiffness index with the CAVI index, assessment of their values and correlations with clinical parameters
Authors: Vasyutin I. A. , Leon K. , Safronova T. A. , Bakholdin I. B. , Barkan V. S. , Milyagin V. A. , Pronko T. P. , Talov A. V. , Tentyukov D. E. , Chulkov V. S. , Peskov K. V. , Podzolkov V. I. , Sokolov V. V. 
Published: February 2023

Aim. To compare the cardio-ankle vascular index (CAVI) and the novel START vascular stiffness index and assess their values and correlations with clinical parameters.Material and methods. This multicenter study included 928 (403 men and 525 women) randomly selected patients, aged 18 to 89 years (mean age, 41±15,8 years). Inclusion criteria were age over 18 years. There were following exclusion criteria: mental disorder, severe somatic diseases and cancer, contraindications for volume sphygmography using the Fukuda Denshi VS-1500 VaSera system, no patient consent, ankle-brachial index <1,0 and >1,3. Further, according to the main parameters obtained using volum sphygmography, a novel START index was calculated. Comparison of index values and analysis of their correlation with clinical indicators, such as age, systolic blood pressure, diastolic blood pressure, pulse pressure (PP), body mass index and heart rate (HR), were carried out using simple and multiple linear regression, dispersion analysis, calculation of the Pearson coefficient (r), in the software environment R version 4.0.2.Results. Statistical analysis revealed a high correlation between START and CAVI indices (r=0,986, p<0,001). The values of both indices increase significantly with age (ANOVA p><0,001). Both START and CAVI correlate with all studied clinical parameters. However, in men, there was no relationship of the indices with PP and HR (p>0,05). According to multiple linear regression, the relationship between diastolic blood pressure at the age of 30-60 years and PP at the age of 18-40 years with the START index is more pronounced than with the CAVI index. However, further study of the mathematical model did not reveal a significant difference in the index values for groups with and without high blood pressure.Conclusion. The START vascular stiffness index significantly correlates with the CAVI index, showing no significant differences from CAVI in quantitative relationships with blood pressure, body mass index, heart rate, and sex in various subgroups of the subjects.
TAVISCORE: a computer program for risk stratification of complications after aortic valve interventions in patients with reduced ejection fraction
Authors: Strelkov D. A. , Zubarev D. D. , Pischugin A. S. , Guryev V. V. , Kulikov D. I. , Postnikova Z. N. , Lening K. S. 
Published: February 2023

Aim. To demonstrate the TAVISCORE program designed to stratify the risk of complications after aortic valve (AV) interventions in patients with reduced ejection fraction.Material and methods. For the period from 2015-2022 at the Almazov Federal North-West Medical Research Center, 128 interventions on AV were performed for aortic stenosis in patients with reduced ejection fraction as follows: 61 — surgical AV replacement (SAVR), 67 — transcatheter aortic valve implantation (TAVI). To create an interactive calculator TAVISCORE (link for free download: https://drive.google.com/file/d/1a3s2MK6Tpk0cIQ_aMB7xe63upEwJsJOh/view?usp=sharing) all patients were combined into one group. For each factor present in the patient, the prognostic coefficient, its contribution to the likelihood of an event in the long-term follow-up period (death, myocardial infarction, stroke), was calculated. The next step, based on the calculations obtained and using the Python 3.10.6 language, was the creation of the TAVISCORE program.Results. The TAVISCORE was created for the personalized choice of tactics for the treatment of patients with aortic stenosis. It contains 54 risk factors and makes it possible to determine probability of cardiac or non-cardiac events in the long-term follow-up period after SAVR and TAVI. Thus, a tactic with lower probability of a complication can be chosen as optimal in this particular case. Retrospective use of the TAVISCORE after surgery can identify patients at high risk of complications, which will allow them to be selected for more thorough management and more frequent screening.Conclusion. The TAVISCORE can be used by a multidisciplinary consensus to select the treatment tactics and stratify the risk of complications after different AV replacement variants in patients with a reduced ejection fraction. Further prospective testing of this program is required.
Cardioprotective effect of thiotriazoline in cancer patients
Authors: Koltsov A. V. , Tyrenko V. V. 
Published: February 2023

Aim. To evaluate the effectiveness of morpholinium-methyl-triazolyl-thioacetate (thiotriazoline) as a cardioprotector in patients with non-Hodgkin’s lymphomas who received chemotherapy with the inclusion of anthracyclines.Material and methods. Fifty patients with non-Hodgkin’s lymphomas were examined on the background of antitumor therapy. The patients were divided into 2 following groups: group 1 (n=27) — standard chemotherapy; group 2 (n=23) — thiotriazoline as a cardioprotector. The quality of life was assessed using the SF-36 questionnaire; the level of troponin I and the natriuretic peptide NT-proBNP was determined; an electrocardiography and echocardiography were performed.Results. In the course of the study, significant differences (p<0,05) were found in following parameters: the severity of dyspnea and lower limb edema, alanine aminotransferase, aspartate aminotransferase, creatine phosphokinase, troponin I level, NT-proBNP, corrected QT interval, end systolic volume, left ventricular ejection fraction, E/A ratio. The results obtained indicate the clinical and paraclinical advantage of thiotriazoline and reflect its cardioprotective effect. Conclusion. The use of thiotriazoline makes it possible to prevent and slow down the cardiovascular disease continuum, leading to the development of heart failure or decompensation that exists in this category of patients. Keywords: cardio-oncology, thiotriazoline, heart failure, anthracyclines, cardiotoxicity>˂0,05) were found in following parameters: the severity of dyspnea and lower limb edema, alanine aminotransferase, aspartate aminotransferase, creatine phosphokinase, troponin I level, NT-proBNP, corrected QT interval, end systolic volume, left ventricular ejection fraction, E/A ratio. The results obtained indicate the clinical and paraclinical advantage of thiotriazoline and reflect its cardioprotective effect.Conclusion. The use of thiotriazoline makes it possible to prevent and slow down the cardiovascular disease continuum, leading to the development of heart failure or decompensation that exists in this category of patients.
Water deficit and salinity modify some morphometric, physiological, and productive attributes of Aloe vera (L.)
Authors: Mota-Ituarte María , Pedroza-Sandoval Aurelio , Minjares-Fuentes Rafael , Trejo-Calzada Ricardo , Zegbe Jorge A , Jesús Josafath Quezada-Rivera  
Published: February 2023

Background: Water deficit and salinity are common conditions in arid agro-ecosystems.
Hypothesis: Salinty increases the hydric stress effect on the yield and gel quality of Aloe vera.
Study species and data description: In A. vera plants the variables meassured were: plant height, leaf length, width, and thickness, fresh biomass, the relative content of water, total solids, gel percentage, and harvest index.
Study site and dates: The study was conducted from spring to summer 2020 in Bermejillo, Durango, Mexico.
Methods: A split-block experimental design with four replications was used. The large plots were two soil moisture contents: field capacity (18.5 ± 2.5 %) and permanent wilting point (12.5 ± 2.5 %). The small plot was the salinity, with levels: 0, 20, 40, 60, and 80 mM of NaCl.
Results:Water deficit and salinity reduced the width and thickness of leaves and, consequently, the fresh biomass and gel percentage were reduced, but gel quality increased.
Conclusions: Water deficit and salinity, mainly the latter, reduced plant growth and leaves, and therefore, the yield of fresh biomass and gel. Although gel yield was reduced, its quality was enhanced, in terms of high total solids content.
Late complications of the new coronavirus infection: a clinical case
Authors: Chepurnenko S. A. , Shavkuta G. V. , Demidova A. A. 
Published: February 2023

According to the literature, exudative pleurisy and pericarditis are considered rare complications of the new coronavirus infection. This estimation can be explained by the fact that statistical studies cover mainly the hospital treatment of this disease. The true frequency of these complications and their consequences are not fully understood.Aim. The study of late complications of the new coronavirus infection in the form of pleurisy and pericarditis.Conclusion. In our case, a 62-year-old patient with the new coronavirus infection confirmed by polymerase chain reaction, severe bilateral polysegmental viral pneumonia, CT3, 60% on day 43 after the onset of clinical symptoms, was found to have manifestations of pleurisy and pericarditis during outpatient treatment. Cardiac MRI is the most informative method for detecting small pericardial and pleural effusions. The diagnostic capabilities of this method are superior to ultrasounography of the heart and pleural cavities and computed tomography of the lungs. Administration of colchicine 1.0 g per day for 1 month allowed not only to the elimination of pericarditis and pleurisy, but also the reduction of pressure in the right ventricle, probably by reducing the damage to the pulmonary parenchyma.