bluuebunny/crossref_metadata_2025_split huggingface dataset:
Untargeted urinary metabolomics for bladder cancer biomarker screening with ultrahigh-resolution mass spectrometry
Authors: Nizioł Joanna , Ossoliński Krzysztof , Płaza-Altamer Aneta , Kołodziej Artur , Ossolińska Anna , Ossoliński Tadeusz , Nieczaj Anna , Ruman Tomasz 
Published: April 2023

Abstract
Bladder cancer (BC) is a common urological malignancy with a high probability of death and recurrence. Cystoscopy is used as a routine examination for diagnosis and following patient monitoring for recurrence. Repeated costly and intrusive treatments may discourage patients from having frequent follow-up screenings. Hence, exploring novel non-invasive ways to help identify recurrent and/or primary BC is critical. In this work, 200 human urine samples were profiled using ultra-high-performance liquid chromatography and ultra-high-resolution mass spectrometry (UHPLC-UHRMS) to uncover molecular markers differentiating BC from non-cancer controls (NCs). Univariate and multivariate statistical analyses with external validation identified metabolites that distinguish BC patients from NCs disease. More detailed divisions for the stage, grade, age, and gender are also discussed. Findings indicate that monitoring urine metabolites may provide a non-invasive and more straightforward diagnostic method for identifying BC and treating recurrent diseases.
What are the determinants of older people adopting communicative e-health services: A meta-ethnography
Authors: Aslan Ayse , Mold Freda , Marwijk Harm van , Armes Jo 
Published: April 2023

Abstract
Background: Gradually, society has shifted more services online, with Covid-19 highlighting digital inequalities in access to services such as healthcare. Particularly older adults can experience such digital inequalities, yet this group is also more likely to need medical appointments, compared to younger people. With the growing digitalisation of healthcare, it is increasingly important to understand how older people can best use communicative e-health services to interact with healthcare services. This is especially if older adults are to access and actively interact with health professionals/clinicians due to their general health decline. This review aims to synthesise older adults’ experiences and perceptions of communicative e-health services and, in turn, to identify barriers and facilitators to using communicative e-health services.
Methods: A meta-ethnography was conducted to qualitatively synthesise literature on older adults’ experiences of using communicative e-health services. A systematic search, with terms relating to ‘older adults’, ‘e-health’, ‘technology’, and ‘communication’, was conducted on six international databases between January 2014 and May 2022. The search yielded a total of 10 empirical studies for synthesis.
Results: The synthesis resulted in 10 themes that may impact older adults’ perceptions and/or experiences of using communicative e-health services. These were: 1) health barriers, 2) support networks, 3) application interface/design, 4) digital literacy, 5) lack of awareness, 6) online security, 7) access to digital devices and the internet, 8) relationship with healthcare provider(s), 9) in-person preference and 10) convenience. These themes interlink with each other.
Conclusion: The findings suggest older adults’ experiences and perceptions of communicative e-health services are generally negative, with many reporting various barriers to engage with online services. However, many of these negative experiences are related to limited support networks and low digital literacy, along with complicated application interfaces. This supports previous literature identifying barriers and facilitators in which older adults experience general technology adoption and suggests a greater emphasis is needed on providing support networks to increase the adoption and usage of communicative e-health services.
Non-thermal effect of terahertz wave radiation on DNA damage repair in living cells
Authors: Harata Masahiko , Ueno Yuya , Yamazaki Shota , Hoshina Hiromichi 
Published: April 2023

Abstract
DNA damage, which is constantly caused by exogenous and endogenous factors, is subject to immediate repair processes; delays in these repair processes cause diseases. Here, we demonstrated that terahertz irradiation enhances the repair processes of DNA double-strand break by a non-thermal effect. The modulation of DNA damage repair by terahertz irradiation provides a non-invasive method with potential medical applications, such as prevention and suppression of diseases caused by DNA instability.
Bidirectional LED as an AC-driven visible-light source
Authors: Żak Mikołaj , Muziol Grzegorz , Siekaz Marcin , Bercha Artem , Hajdel Mateusz , Szkudlarek Krzesimir , Lachowski Artur , Chlipała Mikołaj , Wolny Paweł , Turski Henryk , Skierbiszewski Czeslaw 
Published: April 2023

Abstract
The GaN-based light emitting diodes (LEDs) brought a revolution in the lighting market by becoming the most energy-efficient light sources. However, the power grid, i.e. electricity delivery system, is built based on alternating current (AC), which raises problems for directly driving LEDs that require direct current (DC) to operate effectively. In this paper, we demonstrate a proof of concept device that addresses this fundamental issue – a GaN-based bidirectional light emitting diode (BD LED). The BD LED structure is symmetrical with respect to the active region, which, depending on the negative or positive bias, allows for the injection of either electrons or holes from each side. It is composed of two tunnel junctions that surround the active region. The optical and electrical properties of such BD LEDs under DC and AC conditions are investigated. We find that the light is emitted in both directions of supplied current, contrary to the standard LEDs, hence BD LEDs can be considered as a semiconductor light source powered directly with AC. In addition, we show that BD LEDs can be stacked vertically to multiply the optical power achieved from a single device.
Fetal mosaicism, should conventional karyotype always be performed?
Authors: Su Linjuan , Wu Xiaoqing , Liang Bin , Lin Na , Xie Xiaorui , Cai Meiying , Zheng Lin , Wang Meiying , Xu Liangpu 
Published: April 2023

AbstractThe application of classical cytogenetic and DNA-based molecular techniques to detect cell lineages of mosaicism derived from cultured or non-cultured fetal cells may result in discordant results. This retrospective study aimed to assess the inconsistent diagnostic outcomes, technical availability, and limitations of chromosomal microarray analysis (CMA) and karyotyping for mosaicism. A total of 75 fetuses diagnosed with mosaicism by karyotype analysis or CMA were selected, and the results from both the methods were compared and further analyzed.A total of 42 (56%, 42/75) CMA results were consistent with karyotypes, consisting of 22 cases of mosaic sex chromosomal abnormalities, eight routine autosomal aneuploidy cases, eight other autosome aneuploidy cases, three large cryptic genomic rearrangements, and one small supernumerary marker chromosome. Discrepancy between karyotype analysis and CMA was observed in 33 (44%, 33/75) mosaicisms involving 15 sex chromosomal abnormalities, one routine autosomal aneuploidies, five other autosome aneuploidy cases, eight large cryptic genomic rearrangements and four small supernumerary marker chromosomes. Considering the disparities between methods as well as the cell populations analyzed, both CMA and karyotype analysis have their own advantages and disadvantages. Therefore, CMA should ideally be used in combination with karyotyping to detect more cases of mosaicism than using either test alone.
Case report: Takotsubo syndrome following percutaneous coronary intervention
Authors: Lu Rui , Wang Min , He Shangfei , Lu Jing , Liao Yi , Cui Tongtao , Lu Mingjun 
Published: April 2023

Abstract
Background
Takotsubo syndrome (TTS), which is frequently secondary to severe emotional (fear, anxiety, etc.) or physical stress, is an acute reversible heart failure syndrome characterized by temporary left ventricular regional systolic dysfunction. Nevertheless, TTS after percutaneous coronary intervention (PCI) is rare, and its clinical characteristics are easily confused with complications after PCI.
Case presentation
This article reports a case of TTS induced by psychological and physical pressure after successful PCI in our institution. She had symptoms comparable to complications after PCI, including electrocardiogram (ECG) deviation and troponin elevation. Coronary angiogram, left ventricle contrast echocardiography (LVO), and cardiac magnetic resonance (CMR) were performed to exclude postoperative complications. Definite diagnosis of TTS was eventually achieved.
Conclusion
We cannot dismiss the risk of TTS in patients who have unexplained ECG deviation and troponin elevation following successful PCI. Meanwhile, medical personnel should provide mental, cultural, and emotional services to patients in addition to essential diagnostic and treatment technical services during the perioperative period.
Surface characterization of pulp fiber from mixed old newspaper and magazine deinked-pulp with combined cellulase and laccase-violuric acid system (LVS)
Authors: Akbarpour Iman 
Published: April 2023

AbstractThe pulp and paper industry is looking for eco-friendly solutions in the field of enzymatic deinking for ink elimination from different recycled papers. The current research aimed at deinking secondary fibers containing mixed 70% old newsprint (ONP) and 30% old magazine (OMG) by combining cellulase with the laccase-violuric acid system (LVS). Optical and strength properties, surface chemical composition, fiber crystallinity, fiber morphology changes during the deinking process, and pulp water retention value (WRV) were assessed by electron spectroscopy for chemical analysis (ESCA), fourier transform infrared spectrometry (FTIR), field emission scanning electron microscopy (FESEM), and the centrifuge method. Results revealed the synergistic delignification and deinking effects of cellulase and LVS making enzymatic treatment a promising alternative for chemical deinking. Pulp deinked with cellulase-LVS indicated a lower ERIC (effective residual ink concentration), better optical and strength properties, a higher oxygen/carbon (O/C) ratio of the fiber surface, a lower surface lignin, higher fiber crystallinity index, and improved pulp WRV. Furthermore, FESEM photographs demonstrated that more fibrils appeared on the fiber surface due to synergistic effects between enzymes. Among the combined cellulase-LVS trials, the C2L2trial (0.1% cellulase and 20 min; 40u laccase and 120 min) gave a paper with the best quality, the minor lignin surface coverage (or more lignin degradation), and higher cellulose crystallinity, compared to the chemically deinked pulp.
Effects of stocking density on the homeostasis of uric acid and related liver and kidney functions in ducks
Authors: Lin Peiyi , Liufu Sui , Wang Jinhui , Hou Zhanpeng , Liang Yu , Wang Haiyue , Jin Shaobing , Jiang Danli , Liu Wenjun , Tian Yunbo , Xu Danning , Huang Yunmao , Li Xiujin , Fu Xinliang 
Published: April 2023

Abstract
Background: Avian gout is a common metabolic disease characterized by hyperuricemia and urate deposits in the joints, and it is caused by the overproduction of uric acid by hepatic metabolism or renal underexcretion. However, the effects of stocking density on the metabolism of uric acid are unknown. In the present study, the effects of stocking density on the metabolism and homeostasis of uric acid as well as the related functions of the liver and kidney in ducks were examined. A total of 180 healthy 56-day-old Shan-ma ducks were randomly divided into the low stocking density (LSD; n = 30, density = 5 birds/m2), medium stocking density (MSD; n = 60, density = 10 birds/m2) and high stocking density groups (HSD; n = 90, density = 15 birds/m2). Samples were collected in the 3rd, 6th and 9th weeks of the experiment for analysis.
Results: The results showed that the serum levels of uric acid, LPS and inflammatory cytokines (IL-1β, IL-8 and TNF-α) were increased significantly in the HSD group. Serious histopathological lesions, including acute cellular swelling and vacuolar degeneration of hepatocytes in the liver, swelling of glomeruli, necrosis and abscission of renal tubular epithelial cells, and infiltration of inflammatory cells, could be seen in both the livers and kidneys in the HSD group in the 9th week. In addition, the mRNA expression levels of inflammatory cytokines (IL-8 and TNF-α) and related pathway components (TLR-4, MyD88 and NF-κB) were increased significantly in both the livers and kidneys in the HSD group. Moreover, the mRNA expression levels of enzymes (ADA, XOD, PRPPAT and PRPS1) related to the synthesis of uric acid increased significantly in the livers in the HSD group. However, the mRNA expression level of SLC2A9, which is responsible for the excretion of uric acid by the kidney, was decreased significantly in the kidneys in the HSD group. The effects of stocking density on the functions of the liver and kidney in the HSD group as described above might result in hyperuricemia, which might further induce gout.
Conclusions: In conclusion, these results indicated that a higher stocking density could cause tissue inflammatory lesions in the liver and kidney and subsequently affect the metabolism and homeostasis of uric acid, and is helpful for guiding decisions related to the breeding and production of ducks.
Semi-synthesis and in vitro anti-tumor effects evaluation of novel xanthohumol derivatives
Authors: Sun Xiaolong , Chen Jie , Huang Di , Ding Feng , Zhao Long , Li Hong-Mei , Wang Xiang-Shu , Zhang Yu-Xin , Wu Cheng-Zhu 
Published: April 2023

Abstract
Xanthohumol (Xn) is a chalcone compound isolated from Humulus lupulus Linn. and has various biological activities. In this study, eight Xn derivatives were synthesized by Williamson, Mannich, Reimer-Tiemann, and Schiff base reactions, and five cancer cell lines (MDA-MB-231, MCF-7, CNE-2Z, SMMC-7721, H1975) were evaluated for in vitro cytotoxic activity. Among these, 2-((E)-2,4-dihydroxy-5-((E)-3-(4-hydroxyphenyl)acryloyl)-6-methoxy-3-(3-methylbut-2-en-1-yl)benzylidene)hydrazine-1-carboximidamide (8) exhibited the best potent cytotoxic activity against the five cancer cells, with IC50 values ranging from 4.87 to 14.35 µM. Wound-healing and transwell assays also showed that compound 8 could better inhibit the migration and invasion of MDA-MB-231 cells, and western blotting assays showed that it could reduce protein expression of HIF-1α, MMP-2 and MMP-9. In addition, flow cytometry assays showed that compound 8 could induce apoptosis in MDA-MB-231 cells by up-regulation of Bax and down-regulation of Bcl-2 and Akt expression.
Calibration Method of Clinical Type Infrared Thermometers and Estimation Uncertainty in Different Blackbody Cavities
Authors: Alper Melda Patan 
Published: April 2023

Abstract
In recent years, radiation thermometers or infrared thermometers are frequently preferred in many different sectors from the health sector to the iron and steel industry, food, agriculture, chemistry and automotive, due to their fast measurement capabilities, reasonable prices, wide measurement ranges and practical use. In this study, the calibration systems of infrared clinical radiation thermometers, which are widely used in our hospitals and homes and become more important with the sudden expansion of their usage areas (closed areas, meeting rooms, shopping malls, schools and offices), especially during the Covid-19 period, were investigated. The measurement of human body temperature is an important physiological measurement used primarily for diagnosis, surgery, especially during pandemic diseases such as covid-19, intensive care and treatment procedures. Different types of clinical thermometers are used in body temperature measurement and we can examine these thermometers in two groups: contact thermometers and non-contact thermometers. To have confidence in the accuracy of the measurements of the temperature measuring device, clinical thermometers, it is important that the device is calibrated traceable to the 1990 International Temperature Scale (ITS-90).
Chaotic coexistence of librational and rotational dynamics in the averaged planar three--body problem
Authors: Ruzza Sara Di 
Published: April 2023

Abstract
Through an appropriate change of reference frame and rescalings of the variables and the parameters introduced, the Hamiltonian of the three--body problem is written as a perturbed Kepler problem. In this system, new Delaunay variables are defined and a suitable configuration of the phase space and the mass parameters is chosen. In such a system, wide regions of librational and rotational motions where orbits are regular and stable are found. Close to the separatrix of these regions, the existence of chaotic motions presenting a double rotational and librational dynamics is proved, numerically, through Poincaré sections and the use of FLI.
MSC Classification: 37N05 , 37J12 , 37J40 , 37J46 , 37D10
Outsmarting Android Malware withCutting-edge Feature Engineering andMachine Learning Techniques
Authors: wajahat Ahsan , He Jingsha , Zhu Nafei , Mahmood Tariq , Nazir Ahsan , Qureshi Siraj uddin , Ullah Fahim , Pathan Muhammad Salman 
Published: April 2023

Abstract
The authors have requested that this preprint be removed from Research Square.
Dataset for Vaginal Human Papillomavirus infection among adolescent and early adult girls in Jos, Nigeria
Authors: Cosmas Nanma T. , Nimzing Lohya , Egah Daniel Z. , Famooto Ayotunde O. , Adebamowo Sally N. , Adebamowo Clement A. 
Published: April 2023

Abstract
Objectives: To assess risk factors for HPV infection, determine knowledge about HPV vaccines, assess willingness to receive the HPV vaccine among adolescent and early adult girls in Nigeria, we administered a structured questionnaire. We also collected samples to determine the prevalence and patterns of HPV infections.
Data description: The dataset contains the responses of 205 participants from 10 randomly selected public and private secondary schools in Jos, Nigeria. The data includes information on risk factors for HPV infections such as sexual behaviours, knowledge about HPV vaccine and willingness to receive the vaccine. This is valuable information that can be compared to data from studies in other environments or to determine changes in the pattern of risk factors and HPV prevalence in this population over time.
Infinite System of Nonlinear Tempered FractionalOrder BVPs in the Spaces of Tempered Sequences
Authors: NAGESWARARAO SABBAVARAPU , Khuddush Mahammad , Msmali Ahmed Hussein , Ahmadini Abdullah Ali H. 
Published: April 2023

Abstract
Based on the Hausdorff measure of noncompactness and Meir–Keeler fixed point theorem in a tempered sequence space, we derive sufficient conditions for the existence of solutions via the Hausdorff measure of noncompactness (HMNC) in tempered sequence spaces to the infinite system of Tempered fractional boundary value problem (TFBVP) in the tempered sequence space
Association between suicide attempts and anemia in late-life depression inpatients
Authors: Li Jie , Wang Lan , Sun Yadi , Zhao Fengxue , Lu Ying , Yang Lei 
Published: April 2023

Abstract
Objective: Anemia is strongly associated with late-life depression (LLD); however, few studies have investigated the relationship between anemia and suicide attempts in LLD patients. This study aimed to compare serum ferritin, folate, vitamin B12, and erythrocyte parameter levels in patients with LLD with those in healthy older adults, and the relationship between serum ferritin, folate, vitamin B12, and suicide attempts in patients.
Methods: Serum ferritin, folate, vitamin B12, and erythrocyte parameter levels were measured in hospitalized LLD patients (n=66) and healthy older adults (n=47). All participants were surveyed for basic conditions and suicide attempts, and depression was assessed in LLD patients.
Results: Serum ferritin, folate, vitamin B12, red blood cell count, hemoglobin, and hematocrit levels were significantly lower in LLD patients compared with healthy older adults (p<0.05). Further analysis of the relationship between serum ferritin, folate, and vitamin B12 levels and LLD patients’ suicide attempts and self-assessed depression scores showed a significant negative association between serum folate and vitamin B12 and suicide attempts (p<0.05).
Conclusion: Serum ferritin, folate, vitamin B12, red blood cell count, hemoglobin, and hematocrit levels were significantly lower in LLD patients than in healthy older adults. In addition, reduced serum folate and vitamin B12 levels in patients were associated with suicide attempts.
Integrated metabolic and immune profiling analysis reveals distinct prognostic signatures for neoadjuvant immunotherapy in locally advanced esophageal cancer
Authors: Lu Yuting , Liu Chunquan , Zhao Haiqing , Dong Yin , He Zhihao , Song Shuai , Cui Yong , Jin Jiangtao , Wang Ji Wang , Li Hongzhong , Li Qin 
Published: April 2023

Abstract
Background
There is an unmet demand for non-invasive biomarker assays to identify patients benefiting from neoadjuvant immune checkpoint inhibitors (ICIs). Here, we aimed to characterize the composition and alterations in plasma metabolites and peripheral blood immune cell subtypes associated with neoadjuvant ICI efficacy in locally advanced esophageal cancer (LAEC) and to investigate potential novel therapeutic targets and non-invasive biomarkers for predicting ICI efficacy.
Methods
89 patients with LAEC treated with neoadjuvant programmed cell death 1 blockade combined with chemotherapy were included in this study. We performed an untargeted metabolomic analysis of 606 metabolites on 72 plasma samples using high-performance liquid chromatography-mass spectrometry and an immune profiling analysis of 9 immune cell subtypes on 33 peripheral blood mononuclear-cell samples using flow cytometry. Furthermore, we conducted correlation network and pathway enrichment analyses for potentially beneficial and pathogenic metabolites to explore the metabolite-mediated ICI responses. Finally, a metabolite-based prediction model was established using the least absolute shrinkage and selection operator regression analysis.
Results
Comparative metabolomics revealed that pyrimidine and purine metabolic pathways were disturbed in ICI non-responders, with significant enrichment of dihydrothymidine, ureidoisobutyric acid, and deoxyadenosine, which were significantly associated with poor survival. Conversely, jasmonic acid increased dramatically in responders and was significantly associated with better survival. Strikingly, tryptophan metabolism intermediate-indole-3-acetic acid and arachidonic acid metabolism intermediate-16(R)-HETE levels were positively correlated with cytotoxic T lymphocyte levels but inversely correlated with polymorphonuclear-myeloid-derived suppressor cells levels, which were markedly associated with a favorable prognosis. Notably, the area under the receiver operating curve for the metabolite-based model predicting 12-month overall survival was 87.7% and 82.6% in the discovery and validation cohorts, respectively, demonstrating promising performance.
Conclusions
Our work identified potential non-invasive biomarkers based on plasma metabolic signatures for predicting neoadjuvant ICI responses and prognosis in patients with LAEC, which provides novel insight into ICI precision medicine in the management of LAEC.