bluuebunny/crossref_metadata_2025_split huggingface dataset:
Nanoscale reorganisation of synaptic proteins in Alzheimer's disease
Authors: Zhu Wang‐Hui , Yang Xiao‐Xu , Gou Xu‐Zhuo , Fu Shu‐Mei , Chen Jia‐Hui , Gao Feng , Shen Yong , Bi Dan‐lei , Tang Ai‐Hui 
Published: July 2023

AbstractAimsSynaptic strength depends strongly on the subsynaptic organisation of presynaptic transmitter release and postsynaptic receptor densities, and their alterations are expected to underlie pathologies. Although synaptic dysfunctions are common pathogenic traits of Alzheimer's disease (AD), it remains unknown whether synaptic protein nano‐organisation is altered in AD.Here, we systematically characterised the alterations in the subsynaptic organisation in cellular and mouse models of AD.MethodsWe used immunostaining and super‐resolution stochastic optical reconstruction microscopy imaging to quantitatively examine the synaptic protein nano‐organisation in both Aβ1–42‐treated neuronal cultures and cortical sections from a mouse model of AD, APP23 mice.ResultsWe found that Aβ1–42‐treatment of cultured hippocampal neurons decreased the synaptic retention of postsynaptic scaffolds and receptors and disrupted their nanoscale alignment to presynaptic transmitter release sites. In cortical sections, we found that while GluA1 receptors in wild‐type mice were organised in subsynaptic nanoclusters with high local densities, receptors in APP23 mice distributed more homogeneously within synapses. This reorganisation, together with the reduced overall receptor density, led to reduced glutamatergic synaptic transmission. Meanwhile, the transsynaptic alignment between presynaptic release‐guiding RIM1/2 and postsynaptic scaffolding protein PSD‐95 was reduced in APP23 mice. Importantly, these reorganisations were progressive with age and were more pronounced in synapses in close vicinity of Aβ plaques with dense cores.ConclusionsOur study revealed a spatiotemporal‐specific reorganisation of synaptic nanostructures in AD and identifies dense‐core amyloid plaques as the major local inductor in APP23 mice.
Influence of soil drying followed by flooding on micronutrient solubilization
Authors: Khan Sidra U. , Hooda Peter S. , Blackwell Martin S. A. , Busquets Rosa 
Published: July 2023

AbstractFlooding is known to solubilize soil nutrients, particularly those associated with redox‐sensitive metals (Fe/Mn). Both soil flooding and drying are becoming more common due to climate change, but it is not clear how soil drying prior to flooding influences nutrient solubilization in soils, compared with flooding of already moist soils. This study was designed to examine how soil drying followed by extended flooding might influence solubilization of micronutrient metals (Fe, Mn, Cu, Co, Zn and Ni). A series of laboratory mesocosm experiments was carried out by flooding samples of two contrasting grassland soils, which had each been either dried (40°C for 10 days) or kept at field moisture conditions (25%, w/w). The flooding of dried soils generally resulted in higher concentrations of the micronutrients (Mn, Co, Ni and Cu) in the water columns relative to their moist‐flooded counterparts. The results demonstrate that the flooding‐induced variations in pH and redox potential influence solubilization of micronutrients in the soils. The mobilization of Co and Ni appeared to be controlled by redox‐driven reductive dissolution of Fe/Mn minerals. This was supported by significant (p < .001) negative correlations between redox and metals: Co (r = −.712), Ni (r = −.784) and the positive correlations between Fe and metals: Co (r = .763) and Ni (r = .714) and between Mn and other metals: Co (r = .909) and Ni (r = .811). However, there were no significant correlations of Zn and Cu with Fe and Mn. The results suggest that soil drying followed by flooding has the potential to promote greater solubilization of soil micronutrients compared with flooding of moist soils, with potential implications for soil fertility and catchment water quality under future changes in weather patterns driven by climate change.
Reactive Force Field (ReaxFF) and Universal Force Field Molecular Dynamic Simulation of Solid Electrolyte Interphase Components in Lithium-Ion Batteries
Authors: Nagar Anshul , Garg Akhil , Singh Surinder , Gao Liang , Kim Jonghoon , Wei Kexiang 
Published: July 2023

Abstract
Understanding solid electrolyte interphase (SEI) is essential for the diagnosis of lithium-ion batteries because many aspects of battery performance such as safety and efficiency depend on these characteristics. LiF, Li2O, and Li2CO3 are important inorganic components of SEI. This electrode–electrolyte surface forms during the battery’s first charging/discharging cycle, preventing electrons’ movement through the electrolyte and stabilizing the lithium-ion battery. However, the concern is inorganic SEI components cause rate limitation of lithium-ion diffusivity through the SEI layer. Lithium-ion diffusivity through the SEI layer depends on many factors such as temperature, the width of the SEI layer, and the concentration/density of the layer. Lithium-ion diffusivity dependence on temperature, at working temperatures of lithium-ion batteries was observed at temperatures from 250 K to 400 K and diffusion coefficient data at higher temperatures have also been observed. Lithium-ion diffusivity at varying concentration/density was also observed in this paper using the reactive force field (ReaxFF) molecular dynamic simulation. To improve the lithium-ion diffusivity, vacancy defects were created in the inorganic components of the SEI layer LiF, Li2O, and Li2CO3 and the diffusion coefficient was obtained using the ReaxFF molecular dynamic simulations. Another approach to improve the lithium-ion diffusivity is doping alkali metal ions such Na, Ca, K, and Mg in the inorganic components of SEI layers of LiF, Li2O, and Li2CO3 and simulated using the universal force field (UFF), and the diffusion coefficient was observed.
The 2023 Kahramanmaraş Earthquakes in Turkey: Seismic Movements along Conjugated Faults
Authors: Tikhotsky S. A. , Tatevosyan R. E. , Rebetsky Yu. L. , Ovsyuchenko A. N. , Larkov A. S. 
Published: July 2023

Abstract
The earthquakes in Turkey (Kahramanmaraş province) in February 2023 do not fit into the usual mainshock–aftershocks sequence. According to Bath’s statistical law [1], the magnitude of the strongest aftershock is expected to be one less than the magnitude of the mainshock. Meanwhile, for the aftershock sequence in Turkey, the difference in magnitude is only 0.1. In Turkish publications, the first of the strongest earthquakes is called Pazardzhik (Mw = 7.8) and the second, Elbistan (Mw = 7.7). Each of these earthquakes generated its own system of surface ruptures and aftershock sequences differently oriented in space. The purpose of this study is to assess whether the occurrence of the second earthquake is due to a stress field that existed earlier or if it arose as a result of the mainshock. If the second scenario was realized, the stress field can be almost instantly changed in the vicinity of a strong earthquake (the time difference between the earthquakes was less than nine hours).
Parenting toddlers: Evidences of parental needs from South India
Authors: Holla Chaithra , Thomas Bino , Kishore Madhavaram Thomas 
Published: July 2023

Background: Quality of childcare can predict a child’s emotional and behavioural adjustment throughout the life span. Furthermore, this has major implications for child development and mental health in the long-term. Parents often face major challenges while providing childcare especially during the early years of a child. Aim: This paper describes specific parental concerns in raising a toddler from a mental health developmental perspective. Methods: The study was conducted in urban Bengaluru, India under an exploratory research design using semi-structured interviews. Ten parents participated in the study. Data was analysed using thematic analysis. Results: Four major themes emerged. The first theme was related to the formation of parental beliefs about parenting which identified several sources such as parenting practices witnessed during childhood, childhood experiences and influence of culture, mass or social media. The second theme to emerge was on concerns related to toddlers which parents had difficulties in understanding as well as managing, specially navigating through sleep and feed pattern, identifying developmental needs and making a meaning of toddlers’ communication styles and content. The third theme showed parental concerns which consisted of areas such as parental stress, physical exhaustion, lack of time for each other or toddler, lack of information about handling toddlers and absence of validation of parental efforts. The final theme was on their current parenting practices with their toddlers which showcased controlling styles, play time but low on quality, inappropriate responses to toddlers’ emotions or behaviours and several culturally practiced measures which were not validated scientifically. Conclusion: The pre-existing belief among parents about parenting and toddlers’ specific behaviours influence parenting practices. The understanding of the parental challenges is beneficial in developing an appropriate parenting programme focussing on strengthening psychological capacities of toddlers.
BAFFR expression in circulating T follicular helper (CD4+CXCR5+PD-1+) and T peripheral helper (CD4+CXCR5−PD-1+) cells in systemic lupus erythematosus
Authors: Sagrero-Fabela Nefertari , Ortíz-Lazareno Pablo C , Salazar-Camarena Diana C , Cruz Alvaro , Cerpa-Cruz Sergio , Muñoz-Valle José F , Marín-Rosales Miguel , Alvarez-Gómez Jhonatan A , Palafox-Sánchez Claudia A 
Published: July 2023

Background Circulating T follicular helper (cTfh) and T peripheral helper (Tph) subpopulations are shown to be higher in systemic lupus erythematosus (SLE) patients and have been involved in promoting extrafollicular B cell responses. However, a possible association with the B cell activating factor (BAFF), a cytokine mainly related to B cell responses and disease activity in SLE, has not been investigated. Therefore, this study aimed to evaluate the association of cTfh and Tph subpopulations with the BAFF system expression and clinical activity in SLE patients. Methods This study included 43 SLE patients and 12 healthy subjects (HS). The identification of cTfh (CD4+CXCR5+PD-1+), Tph (CD4+CXCR5−PD-1+) cells, expression of membrane-bound BAFF (mBAFF), BAFFR, TACI, BCMA, and intracellular IL-21 was performed by flow cytometry. Serum levels of IL-21, CXCL13, and BAFF were analyzed using ELISA. The SLEDAI-2K score was used to evaluate disease activity in SLE patients. Results Compared with HS, SLE patients showed a significantly increased percentage of cTfh and Tph cells, higher in patients with clearly active disease. SLE patients had markedly higher IL-21-producing cTfh and Tph cells than HS. Both subpopulations were positively correlated with the disease activity in SLE patients. Tph cells were negatively correlated with CD19+CXCR5+ B cells and positively correlated with CD19+CXCR5− B cells. A low expression of mBAFF and their receptors TACI and BCMA was found on cTfh and Tph cells in SLE patients and HS. However, SLE patients with clearly active disease showed decreased expression of BAFFR on cTfh and Tph subpopulations than patients with mildly active/nonactive disease. Serum IL-21, CXCL13, and BAFF levels were higher in SLE patients than in HS. Levels of CXCL13 were correlated with disease activity. Non-significant correlations were observed among T cell subpopulations and IL-21, CXCL13, and BAFF levels. Conclusions This study emphasizes the importance of cTfh and Tph cells in SLE pathogenesis. Besides the importance of IL-21, our results suggest that BAFFR could play a role in cTfh and Tph subpopulations in the autoimmunity context.
Intercostal Cryoablation During Video-Assisted Lung Resection Can Decrease Postoperative Opioid Use
Authors: Maxwell Conor M. , Weksler Benny , Houda Joseph , Fernando Hiran C. 
Published: July 2023

Objective: Pain requiring opioid use remains an issue even with minimally invasive thoracic surgery. The objective of this study was to investigate the effectiveness of intercostal nerve cryoablation (CRYO) for pain control in adult patients undergoing pulmonary resection. Methods: A retrospective analysis of patients undergoing pulmonary resection by uniportal video-assisted thoracic (uVATS) approach was undertaken. Patients treated with our usual pain regimen (STANDARD) were compared with those who additionally received CRYO. STANDARD includes intercostal bupivacaine, patient-controlled analgesia (24 h), ketorolac (48 to 72 h), and tramadol. Intraoperative CRYO was performed on 5 intercostal levels. The primary aim was to compare pain scores (range, 0 to 10) and morphine equivalent dosages (MED). Secondary outcomes included length of stay, chest tube duration, presence of an air leak, and adverse events. A p value <0.05 was considered significant. Results: There were 49 patients (34 female, 15 male). The median age was 74 (37 to 90) years. Procedures included lobectomy ( n = 32), segmentectomy ( n = 7), and wedge resections ( n = 10). There were 23 (46.9%) CRYO and 26 (53.1%) STANDARD patients. Baseline characteristics were similar. Mean length of stay (2.9 vs 3.5 days), chest tube duration (2.2 vs 1.8 days), and adverse events (9 of 23 vs 7 of 26) were similar. There were no complications attributable to CRYO. Pain scores were not significantly different on postoperative days (POD) 1 to 4. MED was significantly reduced after CRYO on POD 1 (5 vs 47.24), POD 2 (10.93 vs 25.04), POD 3 (8.13 vs 21.7), and POD 4 (7.08 vs 19.17). Conclusions: CRYO can be performed safely during pulmonary resection and can decrease in-hospital opioid use. The results from this retrospective study will need to be validated in future prospective studies.
Median effective dose (ED50) of esketamine combined with propofol for children to inhibit response of gastroscope insertion
Authors: Su Ming , Zhu Yichao , Liu Shupeng , Song Lijuan , Qu Jiangtao , Zhang Yong , Zhang Quanyi 
Published: July 2023

Abstract
Background
Propofol is the most commonly used drug for procedural sedation during gastroscopy. However, independent use of propofol can lead to increased dosage and additional side effects. Esketamine was found to be exceptional in combination with propofol for painless gastroscopy. No studies have calculated the median effective dose (ED50) of esketamine combined with propofol in pediatric painless gastroscopy. Here, we designed a research to study the ED50 of esketamine combined with propofol using the Dixon and Massey up-and-down sequential method for inhibiting the response of gastroscope insertion.

Methods
Children who met the inclusion and exclusion criteria were included in this study. Propofol and esketamine were used as anesthetics for painless gastroscopy in children. To explore the ED50, the initial propofol dose was set at 3 mg/kg in all children. The first child was given an esketamine dose of 0.1 mg/kg, followed by 30 s of slow bolus injection propofol. If anesthesia induction failed (coughing or body movement of children during gastroscope insertion), the esketamine dose was elevated in the next child, with a interval difference of 0.05 mg/kg. Otherwise, if the anesthesia induction was successful, the next dosage was reduced by 0.05 mg/kg. The study was stopped if nine crossover inflection points were reached. The ED50 of esketamine was calculated using probit regression, and the blood pressure, pulse oxygen saturation, heart rate, recovery time, and side effects were recorded in all children.

Results
A total of 26 children were included in this study. The ED50 of esketamine combined with 3 mg/kg propofol was 0.143 mg/kg (95% CI 0.047–0.398 mg/kg). The total consumption of propofol was 16.04 ± 5.37 mg. The recovery time was 16.38 ± 8.70 min. Adverse effects recorded were delayed awakening in two cases and increased oral secretions of another child during the examination inducing cough and hypoxemia (86% was the lowest).

Discussion
The ED50 of esketamine was 0.143 mg/kg when combined with 3 mg/kg propofol for successful sedation in pediatric gastroscope insertion. This sub-anaesthetic dose of esketamine was safe and efficacious with few complications in pediatric painless gastroscopy.

Trial registration
The study was registered at the Chinese Clinical Trial Registry (www.chictr.org.cn; registration number: ChiCTR2100052830 on 06/11/2021).
End-of-life treatment preference discussions between older people and their physician before and during the COVID-19 pandemic: cross sectional and longitudinal analyses from the Longitudinal Aging Study Amsterdam
Authors: Kox Roosmarijne M. K. , Pasman H. Roeline W. , van der Plas Annicka G. M. , Huisman Martijn , Hoogendijk Emiel O. , Onwuteaka-Philipsen Bregje D. 
Published: July 2023

Abstract
Background
COVID-19 could lead to hospitalisation and ICU admission, especially in older adults. Therefore, during the pandemic, it became more important to discuss wishes and preferences, such as older peoples’ desire for intensive treatment in a hospital in acute situations, or not. This study explores what percentage of Dutch older people aged 75 and over discussed Advance Care Planning (ACP) topics with a physician during the first months of the COVID-19 pandemic and whether this was different in these people before the COVID-19 pandemic.

Methods
Data of two ancillary data collections of the Longitudinal Aging Study Amsterdam were used: the LASA 75 PLUS study and the LASA COVID-19 study. The latter provided cross sectional data (during COVID-19; n = 428) and longitudinal data came from participants in both studies (before and during COVID-19; n = 219).

Results
Most older adults had thought about ACP topics during COVID-19 (76,4%), and a minority had also discussed ACP topics with a physician (20.3%). Thinking about ACP topics increased during COVID-19 compared to before COVID-19 in a sample with measurements on both timeframes (82,5% vs 68,0%). Not thinking about ACP topics decreased in the first months of the COVID-pandemic compared to before COVID-19 for all ACP topics together (68.0% vs 82.2%) and each topic separately (hospital 42.0% vs 63.9%; nursing home 36.5% vs 53.3%; treatment options 47.0% vs 62.1%; resuscitation 53.0% vs 70.7%).

Conclusions
Older people do think about ACP topics, which is an important first step in ACP, and this has increased during COVID-19. However, discussing ACP topics with a physician is still not that common. General practitioners could therefore take the initiative in broaching the subject of ACP. This can for instance be done by organizing information meetings.
An overview of innovative living arrangements within long-term care and their characteristics: a scoping review
Authors: Brouwers Mara , Broekharst Damien S.E. , de Boer Bram , Groen Wim G. , Verbeek Hilde 
Published: July 2023

AbstractBackgroundWithin long-term care, a culture change (e.g. focus on increasing autonomy in everyday life) is leading to the development of innovative living arrangements for older adults. Insight into characteristics of innovative living arrangements, which are described as an alternative to regular nursing homes, is lacking. This review aims to provide an overview of innovative living arrangements and to describe their defining characteristics.MethodsA scoping review was performed following the framework of Arksey and O’Malley. The preferred reporting items for systematic reviews and meta-analyses with extension, for scoping reviews (PRISMA-ScR) was also followed. The databases PubMed, PsycInfo, CINAHL, and Web of Science were searched. Articles, published between 2012 and 2023 were included when they presented an innovative living arrangement as an alternative to regular nursing homes. A thematic analysis was performed, describing the physical, social, and organizational environment of the innovative living arrangements.ResultsFifty-six articles were identified describing seven types of distinct innovative living arrangements: small-scale living, the green house model, shared housing arrangements, green care farms, dementia villages, group homes, intergenerational living, and an ‘other’ category. The themes included supporting autonomy and creating a small-scale and/or homelike environment, which were emphasized in most innovative living arrangements. Other themes, such as involvement of the community, focus on nature, integration of work tasks, and involvement of family members, were emphasized in a subsection of the described living arrangements. Twenty-eight articles reported on the effects of the innovative living environment on residents, family members, or staff members. Most articles (N = 22) studied resident-related outcomes, focusing mainly on quality of life and aspects of daily life.ConclusionMore insight into the mechanisms of the social and organizational environments is needed, which may lead to greater transparency and homogeneity regarding the description of living arrangements. This review shows that more knowledge is needed about the potential key elements of innovative living arrangements, especially related to their social and organizational environment. This may provide a better guide for developers within long-term care.
New medicines for spontaneous preterm birth prevention and preterm labour management: landscape analysis of the medicine development pipeline
Authors: McDougall Annie R. A. , Hastie Roxanne , Goldstein Maya , Tuttle Andrew , Ammerdorffer Anne , Gülmezoglu A. Metin , Vogel Joshua P. 
Published: July 2023

Abstract
Background
There are few medicines in clinical use for managing preterm labor or preventing spontaneous preterm birth from occurring. We previously developed two target product profiles (TPPs) for medicines to prevent spontaneous preterm birth and manage preterm labor. The objectives of this study were to 1) analyse the research and development pipeline of medicines for preterm birth and 2) compare these medicines to target product profiles for spontaneous preterm birth to identify the most promising candidates.

Methods
Adis Insight, Pharmaprojects, WHO international clinical trials registry platform (ICTRP), PubMed and grant databases were searched to identify candidate medicines (including drugs, dietary supplements and biologics) and populate the Accelerating Innovations for Mothers (AIM) database. This database was screened for all candidates that have been investigated for preterm birth. Candidates in clinical development were ranked against criteria from TPPs, and classified as high, medium or low potential. Preclinical candidates were categorised by product type, archetype and medicine subclass.

Results
The AIM database identified 178 candidates. Of the 71 candidates in clinical development, ten were deemed high potential (Prevention: Omega-3 fatty acid, aspirin, vaginal progesterone, oral progesterone, L-arginine, and selenium; Treatment: nicorandil, isosorbide dinitrate, nicardipine and celecoxib) and seven were medium potential (Prevention: pravastatin and lactoferrin; Treatment: glyceryl trinitrate, retosiban, relcovaptan, human chorionic gonadotropin and Bryophyllum pinnatum extract). 107 candidates were in preclinical development.

Conclusions
This analysis provides a drug-agnostic approach to assessing the potential of candidate medicines for spontaneous preterm birth. Research should be prioritised for high-potential candidates that are most likely to meet the real world needs of women, babies, and health care professionals.