bluuebunny/crossref_metadata_2025_split huggingface dataset:
68Ga-DOTATOC PET/CT Imaging of a Muscular Solitary Fibrous Tumor
Authors: Richard Capucine , Cros Jérôme , Seban Romain-David , Champion Laurence , Hescot Ségolène 
Published: March 2023

Abstract
A 73-year-old woman was referred for 68Ga-DOTATOC PET/CT staging of a grade 2 pancreatic neuroendocrine tumor, which showed the primary pancreatic tumor, liver metastases, one left pleural metastasis, and high uptake in a mass of the right triceps brachii muscle. Two years before, she underwent 18F-FDG PET/CT and 111In-pentetreotide scan, respectively, with low and high uptake of each radiotracer in the triceps mass. Histopathological analysis revealed a solitary fibrous tumor. Immunohistochemistry showed no staining for SSTR-2 and SSTR-5, suggesting tumor overexpression of another somatostatin receptor. This case highlighted a potential pitfall on 68Ga-DOTATOC PET/CT.
Metabolomics Differences of the Donor Livers Between In Situ and Ex Situ Conditions During Ischemia-free Liver Transplantation
Authors: Guo Zhiyong , Zhan Liqiang , Gao Ningxin , Zhang Zhiheng , Huang Shanzhou , Wang Linhe , Zhu Caihui , Jia Zehua , Yin Meixian , Li Fangcong , Chen Shirui , Luo Tao , Liu Yao , Jia Yu , Wang Tielong , Xu Jinghong , Li Yuexin , Zhu Yifan , Chen Yichao , Yu Chuhan , Tang Yunhua , Zhao Qiang , Wang Dongping , He Xiaoshun 
Published: March 2023

Background.
Ischemia-free liver transplantation (IFLT) has been innovated to avoid graft ischemia during organ procurement, preservation, and implantation. However, the metabolism activity of the donor livers between in the in situ and ex situ normothermic machine perfusion (NMP) conditions, and between standard criteria donor and extend criteria donor remains unknown.

Methods.
During IFLT, plasma samples were collected both at the portal vein and hepatic vein of the donor livers in situ during procurement and ex situ during NMP. An ultra-high performance liquid chromatography-mass spectrometry was conducted to investigate the common and distinct intraliver metabolite exchange.

Results.
Profound cysteine and methionine metabolism, and aminoacyl-tRNA biosynthesis were found in both in situ and ex situ conditions. However, obvious D-arginine and D-ornithine metabolism, arginine and proline metabolism were only found in the in situ condition. The suppressed activities of the urea cycle pathway during ex situ condition were confirmed in an RNA expression level. In addition, compared with extend criteria donor group, standard criteria donor group had more active intraliver metabolite exchange in metabonomics level. Furthermore, we found that the relative concentration of p-cresol, allocystathionine, L-prolyl-L-proline in the ex situ group was strongly correlated with peak alanine aminotransferase and aspartate aminotransferase at postoperative days 1–7.

Conclusions.
In the current study, we show the common and distinct metabolism activities during IFLT. These findings might provide insights on how to modify the design of NMP device, improve the perfusate components, and redefine the criteria of graft viability.
Sociodemographic and Geographic Variation in Access to Neuro-Ophthalmologists in the United States
Authors: Xue Katie , Feng Yilin , Tam Vicky , Lin Chun Chieh , De Lott Lindsey B. , Hamedani Ali G. 
Published: March 2023

Background:
Neuro-ophthalmologists have expertise in rare and complex disorders, but the ability of patients to access neuro-ophthalmic care has not been examined at a nationwide level.

Methods:
Using the 2020 directory of all 502 members of the North American Neuro-Ophthalmology Society as a reference, we found the practice locations of 461 confirmed practicing members and converted each street address to latitude and longitude coordinates. We calculated the travel distance and time from each census tract to the nearest practice location and calculated population-weighted averages by state, region, and other prespecified factors. Choropleth maps were used to visualize the distribution of travel distances and times across the United States.

Results:
California had the most practicing neuro-ophthalmologists out of any state (50), whereas 4 states (DE, MT, SD, and WY) had none. Washington, DC and MA had the most neuro-ophthalmologists per capita. The average travel distance and time to the nearest neuro-ophthalmologists were found to be 40.90 miles and 46.50 minutes, respectively, although a large portion of western plains and mountain regions had travel times of over 120 minutes. Patients in rural areas had longer travel times than those in urban areas, and Native American patients had the longest travel times of any racial or ethnic group.

Conclusion:
The travel time to see a neuro-ophthalmologist varies widely by state, region, and rurality, with Native American patients and rural patients being disproportionately affected. By identifying the areas with the greatest travel burdens, future policies can work to alleviate these potential barriers to care.
Technological Solutions for Urinary Continence Care Delivery for Older Adults
Authors: Omotunde Muyibat , Wagg Adrian 
Published: March 2023

PURPOSE:
The aim of this scoping review was to examine available evidence regarding use of technology-based continence care delivery for older adults and to identify gaps in knowledge.

METHODS:
Scoping review.

SEARCH STRATEGY:
With the help of a medical librarian, CINAHL, Cochrane Library, EMBASE, MEDLINE, ProQuest, PubMed, SCOPUS, Web of Science, and websites were searched. Search terms included technology, sensors, older adults, urinary incontinence, continence care, nursing homes, long-term care, and continence management. All literature elements except for opinion pieces and case reports written in English within the last 15 years were included. Articles not written in the English language were excluded; our search indicated that less than 6% of returned elements were written in other languages.

FINDINGS:
After duplications were removed, 2146 potential sources were identified. After exclusions, 19 results were included in the review. Review findings suggest positive effects of technology-based continence care on older adults and those involved in their care such as enhanced delivery of a successful toileting program. Information on potential harms, from either the perspective of care provider or recipient, is limited. It is important that needs of older adults and collaborative efforts are considered in the implementation of technology-based continence care. A paucity of guidelines on the use and adoption of technology-based continence care was found; additional research into uptake and sustainability is needed.

CLINICAL IMPLICATIONS:
Technological solutions, such as sensors, need to be accurate in the measurement of urine saturation levels and timely in notifying caregivers for effective delivery of continence care. Adverse consequences of incontinence, such as incontinence-associated dermatitis or urinary tract infection, may potentially be reduced or avoided with technology-based continence care delivery.
TEAD1 is crucial for developmental myelination, Remak bundles, and functional regeneration of peripheral nerves
Authors: Grove Matthew , Kim Hyukmin , Pang Shuhuan , Amaya José Paz , Hu Guoqing , Zhou Jiliang , Lemay Michel , Son Young-Jin 
Published: March 2023

ABSTRACTPreviously we showed that the hippo pathway transcriptional effectors, YAP and TAZ, are essential for Schwann cells (SCs) to develop, maintain and regenerate myelin (Grove et al., 2017; Grove, Lee, Zhao, & Son, 2020). Although TEAD1 has been implicated as a partner transcription factor, the mechanisms by which it mediates YAP/TAZ regulation of SC myelination are unclear. Here, using conditional and inducible knockout mice, we show that TEAD1 is crucial for SCs to develop and regenerate myelin. It promotes myelination by both positively and negatively regulating SC proliferation, enabling Krox20/Egr2 to upregulate myelin proteins, and upregulating the cholesterol biosynthetic enzymes FDPS and IDI1. We also show stage-dependent redundancy of TEAD1 and that non-myelinating SCs have a unique requirement for TEAD1 to enwrap nociceptive axons in Remak bundles. Our findings establish TEAD1 as a major partner of YAP/TAZ in developmental myelination and functional nerve regeneration and as a novel transcription factor regulating Remak bundle integrity.
Genetic influences vary by age and sex: Trajectories of the association of cholinergic system variants and theta band event related oscillations
Authors: Chorlian David B. , Meyers Jacquelyn L. , Manz Niklas , Zhang Jian , Kamarajan Chella , Pandey Ashwini , Wang Jen-Chyong , Plawecki Martin , Edenberg Howard , Goate Alison , Tischfield Jay , Porjesz Bernice 
Published: March 2023

AbstractTo characterize systemic changes in genetic effects on brain development, the age variation of the associations of cholinergic genetic variants and theta band event-related oscillations (EROs) was studied in a sample of 2140 adolescents and young adults, ages 12 to 25 from the COGA prospective study. The theta band EROs were elicited in visual and auditory oddball (target detection) tasks and measured by EEG recording. Associations were found to vary with age, sex, task modality (auditory or visual), and scalp locality. Seven of the twenty-one muscarinic and nicotinic cholinergic SNPs studied in the analysis, fromCHRM2, CHRNA3, CHRNA5, andCHRNB4, had significant effects on theta band EROs with considerable age spans for some sex-modality combination. No SNP-age-modality combination had significant effects in the same direction for males and females. Results suggest that nicotinic receptor associations are stronger before age 18, while muscarinic receptor associations are stronger after age 18.
Mass-immigration shapes the antibiotic resistome of wastewater treatment plants
Authors: Zhang Lanping , Adyari Bob , Hou Liyuan , Yang Xiaoyong , Gad Mahmoud , Wang Yuwen , Ma Cong , Sun Qian , Tang Qiang , Zhang Yifeng , Yu Chang-Ping , Hu Anyi 
Published: March 2023

AbstractWastewater treatment plants (WWTPs) are the hotspots for the spread of antibiotic resistance genes (ARGs) into the environment. Nevertheless, a comprehensive assessment of the city-level and short-term daily variations of ARG surveillance is still lacking in WWTPs. Here, 285 ARGs and ten mobile gene elements (MGEs) were monitored in seven WWTPs in Xiamen via high-throughput qPCR (HT-qPCR) for seven days. The average daily load of ARGs to WWTPs was about 1.21 × 1020copies/d, and a total of 1.44 × 1018copies/d was discharged to the environment across the entire city. Interestingly, no daily variations were observed in ARG richness, abundance, and community composition. Stochastic processes were the main force determining the assembly of ARG communities, with their relative importance ranked in the order of influent (INF) > effluent (EFF) > activated sludge (AS). Further analyses indicated that bacteria and ARGs from upstream treatment units played an increasingly dominant role in shaping ARG communities in AS and EFF, respectively, suggesting the importance of mass-immigration of bacteria and ARGs from the source on ARG transport in wastewater treatment units. This emphasizes the need to revise the way we mitigate ARG contamination but focus on the source of ARGs in urban wastewater.
Transposable element RNA dysregulation in mutant KRAS(G12C) 3D lung cancer spheroids
Authors: Carrillo David , Reggiardo Roman E. , Lim John , Mantalas Gary , Peddu Vikas , Kim Daniel H. 
Published: March 2023

ABSTRACTMutant KRAS regulates transposable element (TE) RNA and interferon-stimulated gene (ISG) expression, but it remains unclear whether diverse mutations in KRAS affect different TE RNAs throughout the genome. We analyzed the transcriptomes of 3D human lung cancer spheroids that harbor KRAS(G12C) mutations to determine the landscape of TE RNAs regulated by mutant KRAS(G12C). We found that KRAS(G12C) signaling is required for the expression of LINE- and LTR-derived TE RNAs that are distinct from TE RNAs previously shown to be regulated by mutant KRAS(G12D) or KRAS(G12V). Moreover, KRAS(G12C) inhibition specifically upregulates SINE-derived TE RNAs from the youngest Alu subfamily AluY. Our results reveal that TE RNA dysregulation in KRAS-driven lung cancer cells is mutation-dependent, while also highlighting a subset of young, Alu-derived TE RNAs that are coordinately activated with innate immunity genes upon KRAS(G12C) inhibition.
Modeling the marmoset brain using embryonic stem cell-derived cerebral assembloids
Authors: Kodera Tomoki , Takeuchi Ryosuke F. , Takahashi Sara , Suzuki Keiichiro , Kassai Hidetoshi , Aiba Atsu , Shiozawa Seiji , Okano Hideyuki , Osakada Fumitaka 
Published: March 2023

AbstractStudying the non-human primate (NHP) brain is required for the translation of rodent research to humans, but remains a challenge for molecular, cellular, and circuit-level analyses in the NHP brain due to the lack ofin vitroNHP brain system. Here, we report anin vitroNHP cerebral model using marmoset (Callithrix jacchus) embryonic stem cell-derived cerebral assembloids (CAs) that recapitulate inhibitory neuron migration and cortical network activity. Cortical organoids (COs) and ganglionic eminence organoids (GEOs) were induced from cjESCs and fused to generate CAs. GEO cells expressing the inhibitory neuron marker LHX6 migrated toward the cortical side of CAs. COs developed their neural activity from a synchronized pattern to an unsynchronized pattern as COs matured. CAs showed mature neural activity with an unsynchronized pattern. The marmoset assembloid system will provide anin vitroplatform for the NHP neurobiology and facilitate translation into humans in neuroscience research, regenerative medicine, and drug discovery.
Technical upgrade of an open-source liquid handler to support bacterial colony screening
Authors: del Olmo Irene , Yubero Pablo , Gómez-Luengo Álvaro , Nogales Juan , Espeso David R. 
Published: March 2023

AbstractThe optimization of genetically engineered biological constructs is a key step to deliver high-impact biotechnological applications. The use of high-throughput DNA assembly methods allows the construction of enough genotypic variants to successfully cover the target design space. This, however, entails extra workload for researchers during the screening stage of candidate variants. Despite the existence of commercial colony pickers, their high price excludes small research laboratories and budget-adjusted institutions from accessing such extensive screening capability. In this work we present COPICK, a technical solution to automatize colony picking in an open-source liquid handler Opentrons OT-2. COPICK relies on a mounted camera to capture images of regular Petri dishes and detect microbial colonies for automated screening. COPICK’s software can then automatically select the best colonies according to different criteria (size, color and fluorescence) and execute a protocol to pick them for further analysis. Benchmark tests performed forE. coliandP. putidacolonies delivers a raw picking performance over pickable colonies of 82% with an accuracy of 73.4% at an estimated rate of 240 colonies/h. These results validate the utility of COPICK, and highlight the importance of ongoing technical improvements in open-source laboratory equipment to support smaller research teams.
The chemical landscape of the human ribosome at 1.67 Å resolution
Authors: Faille Alexandre , Dent Kyle C. , Pellegrino Simone , Jaako Pekka , Warren Alan J 
Published: March 2023

ABSTRACTThe ability of ribosomes to translate the genetic code into protein requires a finely tuned ion and solvent ecosystem. However, the lack of high-resolution structures has precluded accurate positioning of all the functional elements of the ribosome and limited our understanding of the specific role of ribosomal RNA chemical modifications in modulating ribosome function in health and disease. Here, using a new sample preparation methodology based on functionalised pristine graphene-coated grids, we solve the cryo-EM structure of the human large ribosomal subunit to a resolution of 1.67 Å. The accurate assignment of water molecules, magnesium and potassium ions in our model highlights the fundamental biological role of ribosomal RNA methylation in harnessing unconventional carbon-oxygen hydrogen bonds to establish chemical interactions with the environment and fine-tune the functional interplay with tRNA. In addition, the structures of three translational inhibitors bound to the human large ribosomal subunit at better than 2 Å resolution provide mechanistic insights into how three key druggable pockets of the ribosome are targeted and illustrate the potential of this methodology to accelerate high-throughput structure-based design of anti-cancer therapeutics.
The acetylase activity of Cdu1 regulates bacterial exit from infected cells by protectingChlamydiaeffectors from degradation
Authors: Bastidas Robert J. , Kędzior Mateusz , Davidson Robert K. , Walsh Stephen C. , Dolat Lee , Sixt Barbara S. , Pruneda Jonathan N. , Coers Jörn , Valdivia Raphael H. 
Published: March 2023

AbstractMany cellular processes are regulated by ubiquitin-mediated proteasomal degradation. Pathogens can regulate eukaryotic proteolysis through the delivery of proteins with de-ubiquitinating (DUB) activities. The obligate intracellular pathogenChlamydia trachomatissecretes Cdu1 (ChlaDUB1), a dual deubiquitinase and Lys-acetyltransferase, that promotes Golgi remodeling and survival of infected host cells presumably by regulating the ubiquitination of host and bacterial proteins. Here we determined that Cdu1’s acetylase but not its DUB activity is important to protect Cdu1 from ubiquitin-mediated degradation. We further identified threeC. trachomatisproteins on the pathogen-containing vacuole (InaC, IpaM, and CTL0480) that required Cdu1‘s acetylase activity for protection from degradation and determined that Cdu1 and these Cdu1-protected proteins are required for optimal egress ofChlamydiafrom host cells. These findings highlight a non-canonical mechanism of pathogen-mediated protection of virulence factors from degradation after their delivery into host cells and the coordinated regulation of secreted effector proteins.
Dimensionality and ramping: Signatures of sentence integration in the dynamics of brains and deep language models
Authors: Desbordes Théo , Lakretz Yair , Chanoine Valérie , Oquab Maxime , Badier Jean-Michel , Trébuchon Agnès , Carron Romain , Bénar Christian-G. , Dehaene Stanislas , King Jean-Rémi 
Published: March 2023

AbstractA sentence is more than the sum of its words: its meaning depends on how they combine with one another. The brain mechanisms underlying such semantic composition remain poorly understood. To shed light on the neural vector code underlying semantic composition, we introduce two hypotheses: First, the intrinsic dimensionality of the space of neural representations should increase as a sentence unfolds, paralleling the growing complexity of its semantic representation, and second, this progressive integration should be reflected in ramping and sentence-final signals. To test these predictions, we designed a dataset of closely matched normal and Jabberwocky sentences (composed of meaningless pseudo words) and displayed them to deep language models and to 11 human participants (5 men and 6 women) monitored with simultaneous magneto-encephalography and intracranial electro-encephalography. In both deep language models and electrophysiological data, we found that representational dimensionality was higher for meaningful sentences than Jabberwocky. Furthermore, multivariate decoding of normal versus Jabberwocky confirmed three dynamic patterns: (i) a phasic pattern following each word, peaking in temporal and parietal areas, (ii) a ramping pattern, characteristic of bilateral inferior and middle frontal gyri, and (iii) a sentence-final pattern in left superior frontal gyrus and right orbitofrontal cortex. These results provide a first glimpse into the neural geometry of semantic integration and constrain the search for a neural code of linguistic composition.Significance statementStarting from general linguistic concepts, we make two sets of predictions in neural signals evoked by reading multi-word sentences. First, the intrinsic dimensionality of the representation should grow with additional meaningful words. Second, the neural dynamics should exhibit signatures of encoding, maintaining, and resolving semantic composition. We successfully validated these hypotheses in deep Neural Language Models, artificial neural networks trained on text and performing very well on many Natural Language Processing tasks. Then, using a unique combination of magnetoencephalography and intracranial electrodes, we recorded high-resolution brain data from human participants while they read a controlled set of sentences. Time-resolved dimensionality analysis showed increasing dimensionality with meaning, and multivariate decoding allowed us to isolate the three dynamical patterns we had hypothesized.
To whom do restaurants’ preventive measures matter more during a pandemic? The moderating effects of need-for-cognition and relationship strength
Authors: Kim Heewon , Jang SooCheong (Shawn) , Gim Jaehee 
Published: March 2023

Purpose
Does every customer respond in the same way to restaurants’ preventive measures (PM)? To answer this question, the purpose of the present study was to examine the moderating role of need-for-cognition (NC) and customer-restaurant relationship strength (RS) in the impact of restaurants’ PM on dine-in intentions using a trust (Study 1) and psychological discomfort (Study 2) as mediators.

Design/methodology/approach
Two studies were conducted using a 2 (PM: present vs control) × 2 (RS: strong vs weak) factorial design. NC was treated as a continuous variable in the regression model.

Findings
The results of two experimental studies showed that people with a high NC are more likely to show positive responses (higher trust and less discomfort) to restaurants’ PM when they have strong relationships with the restaurant. In contrast, people with a low NC did not show any interaction between PM and RS for trust and discomfort.

Practical implications
For restaurants targeting people with a high NC and with more returning customers than new customers, the study results suggest that safety measures should be promoted.

Originality/value
The present study expands the knowledge of customers’ reactions to restaurants’ PM by using the theoretical foundation of the ELM. The results of this study contribute to hospitality research by demonstrating the differences in customers’ thought processes according to their NC and the strength of their relationship with the restaurant.