bluuebunny/crossref_metadata_2025_split huggingface dataset:
Blockade of the Retinal Orexin Receptors Affects Retinal and Hypothalamic C-fos Expression and Hypothalamic Rhythmicity in Male Wistar Rats
Authors: Haddad Muhammad , Khazali Homayoun , Janahmadi Mahyar , Ghanbarian Hossein 
Published: September 2023

Abstract
Orexin A and B (OXA and OXB) and their receptors are expressed in the majority of retinal neurons in humans, rats, and mice. Orexins modulate signal transmission between the different layers of the retina. The suprachiasmatic nucleus (SCN) and the retina are central and peripheral components of the body's biological clocks; respectively. The SCN receives photic information from the retina through the retinohypothalamic tract (RHT) to synchronize bodily functions with environmental changes. In present study, we aimed to investigate the impact of inhibiting retinal orexin receptors on the expression of retinal Bmal1 and C-fos, as well as hypothalamic C-fos, Bmal1, Vip, and PACAP at four different time-points (Zeitgeber time; ZT 3, 6, 12, and ZT-24). The intravitreal injection (IVI) of OX1R antagonist (SB-334867) and OX2R antagonist (JNJ-10397049) significantly up-regulated C-fos expression in the retina. Additionally, compared to the control group, the combined injection of SB-334867 and JNJ-10397049 showed a greater increase in retinal expression of this gene. In addition, the expression of hypothalamic Vip and PACAPwas significantly up-regulated in both the SB-334867 and JNJ-10397049 groups. In contrast, the expression of Bmal1 was down-regulated. Finally, the expression of hypothalamic C-fos was down-regulated in all groups treated with SB-334867 and JNJ-10397049. This study is the first to investigate the impact of retinal orexin receptors on regulating rhythmicity in the retina and hypothalamus. These findings could give a new dimension to the understanding of disorders associated with circadian imbalances such as sleep, and the development of neurodegenerative disorders, like Alzheimer's.
Systematic analyses of the relationship between five CXCL8 gene polymorphisms and overall cancer risk
Authors: Peng Jie , Wang Yinan , Kuang Dan , Wang Ying , Wu Gang , Li Huangjing , Li Dan , Cao Hong 
Published: September 2023

Abstract
Background
Interleukin-8 (IL-8/CXCL8) is a prototypical CXC family chemokine bearing an ELR motif that plays key roles in the onset and progression of a range of cancers in humans. Many prior studies have focused on exploring the relationship between CXCL8 gene polymorphisms and the risk of cancer. However, the statistical power of many of these reports was limited, yielding ambiguous or conflicting results in many cases.
Methods
Accordingly, the present meta-analysis of 28 related publications was conducted in an effort to better understand the association between these mutations and disease risk.
Results
The CXCL8 -353A/T polymorphism was associated with an increased overall cancer risk [A vs. T, odds ratio (OR) = 1.255, 95% confidence interval (CI) (1.079–1.459), Pheterogeneity = 0.449, P = 0.003]. The CXCL8 + 781T/C allele was similarly associated with a higher risk of cancer among Caucasians [TT vs. TC + CC, OR = 1.472, 95%CI (1.078–2.009), Pheterogeneity = 0.366, P = 0.015]. To better clarify the relationship between these mutations and clinical outcomes, tissue samples from oral cancer patients and healthy controls were analyzed. In these analyses, oral cancer patients carrying the CXCL + 781 TT + TC genotypes exhibited pronounced increases in serum levels of CXCL8 as compared to patients carrying the CC genotype (P < 0.01), and CXCL8 levels were also significantly higher in oral cancer patients with these two genotypes as compared to genotype-matched normal controls (P < 0.01).
Conclusion
These results thus suggest that the CXCL8-353 and + 781 polymorphisms may be associated with a greater risk of cancer development. The + 781 polymorphism may further offer value as a biomarker that can aid in the early identification and prognostic evaluation of oral cancer.
Acute Caval Thrombosis Leading to Obstructive Shock in the Early Post Insertion Period of an Inferior Vena Cava Filter ： A Case Report and Literature Review
Authors: Gao ShuTing , Chen YunFei , Huang YaTing , Dang YiPing , Li YiQing 
Published: September 2023

Abstract
Background
Obstructive shock is extremely rare in clinical practice and is caused by acute blood flow obstruction in the central vessels of either the systemic or pulmonary circulation. Utilizing inferior vena cava filters (IVCFs) to prevent pulmonary embolism (PE) is associated with some potential complications, such as inferior vena cava thrombosis (IVCT). Shock as a direct result of IVCT is rare. We present a case of obstructive shock secondary to extensive IVCT caused by inadequate anticoagulant therapy after the placement of an IVCF.
Case presentation:
A 63-year-old male patient with a traffic accident injury presented orthopaedic trauma and lower limb deep vein thrombosis (DVT). He experienced sudden and severe abdominal pain with hypotension, tachycardia, tachypnea, oliguria and peripheral oedema 5 days after IVCF placement and 3 days after cessation of anticoagulant therapy. Considering that empirical anti-shock treatment lasted for a while and the curative effect was poor, we finally recognized the affected vessels and focused on the reason for obstructive shock through imaging findings—inferior vena cava thrombosis and occlusion. The shock state immediately resolved after thrombus aspiration. The same type of shock occurred again 6 days later during transfer from the ICU to general wards and the same treatment was administered. The patient recovered smoothly in the later stage, and the postoperative follow-up at 1, 3, and 12 months showed good results.
Conclusion
This case alerts clinicians that it is crucial to ensure adequate anticoagulation therapy after IVCF placement, and when a patient presents with symptoms such as hypotension, tachycardia, and lower limb and scrotal oedema postoperatively, immediate consideration should be given to the possibility of obstructive shock, and prompt intervention should be based on the underlying cause.
Moringa oleifera Genebank in Brazil: current status and future approaches
Authors: Soares Tássia Fernanda Santos Neri , Gois Itamara Bomfim , Souza Juliana Lopes , Muniz Evandro Neves , Silva Ana Veruska Cruz 
Published: September 2023

Abstract
Moringa oleifera Lam. is a tropical tree that belongs to the Moringaceae family, and it is popularly known worldwide for its multiple applications. This study aimed to evaluate the genetic variability of individuals from the Moringa Genebank of Embrapa Tabuleiros Costeiros, Sergipe, Brazil. The Moringa Genebank is composed of 25 accessions, represented by 177 genotypes, of which 18 were transferred from an exchanged germplasm of the University of Florida, USA, and the others were from different states of Brazil. Leaves of each genotype were collected for DNA extraction and PCR analysis using 20 ISSR primers. A total of 144 bands were amplified and 100% of them were polymorphic. The average of expected heterozygosity (He) and Shannon’s Index was 0.11 and 0.12, respectively. The highest genetic divergence was found between M4 and M18 accessions, both from Florida, USA. Whereas the closest pair of accession was M23 and M24, both from Brazil. The cluster analysis obtained through the Structure software divided moringa genotypes into two groups. Taken together, these results suggest low genetic diversity between the accessions of the Moringa Genebank. Therefore, the introduction of new accessions in the Moringa GeneBank is essential to increase the genetic variability of the species to ensure its conservation and improvement.
Instance Weighting Domain Adaptive Deep Transfer Learning for Spectrum Sensing in Cognitive Radio Network
Authors: Muthukumaran D. , Sharanya C. , Natarajan Rajesh , Najmusher H , Sebastian George , Gowthami V 
Published: September 2023

Abstract
Cognitive radio allowed the licensed vacant spectrum sharing to unlicensed users depending on the radio scene analysis. Cognitive radio performed machine-to-machine communication. Radio scene analysis is carried out in spectrum sensing, interference and channel estimation. Cognitive radio increased the spectrum utilization in wireless communication. Spectrum sensing is an essential component of cognitive radio based on feature extraction of received signal at specified point. Many existing methods are discussed for performing efficient spectrum sensing. But, the spectrum sensing accuracy was not improved and time complexity was not minimized by existing methods. The transfer learning has an opportunity to increase spectrum sensing accuracy through cooperative spectrum sensing and to analyze the radio scene. In order to improve the accuracy and reduce the time complexity, Instance Weighting Domain Adaptive Deep Transfer Learning (IWDADTL) Method is introduced based on data features, patterns and temporal characteristics. IWDADTL Method comprised five layers, namely one input layer, three hidden layers and one output layer to identify the presence or absence of signal at specified location. The designed method has three stages in three hidden layers, namely data splitting, phase selection and fine tuning. In IWDADTL Method, data splitting stage is carried out to divide the data into source data and target data. The source data is used for training model where positive values are paramount. Target data is used for fine-tuning the negative value from − 20dB to − 2dB where majority values are negative. The data splitting is used to tackle unstable training of noisy signals to attain efficient performance accuracy. In phase selection stage, signal components (i.e., in-phase and quadrature-phase) are selected for training, validation and testing on source data. In second stage, an optimal structure is obtained through hyperparameter tuning. Then, IWDADTL Method is fine-tuned on target data to improve the accuracy results and to reduce time complexity. The results of IWDADTL Method show the improvement in spectrum sensing in terms of accuracy, precision and recall.
A comprehensive bioinformatics algorithm and experiment-based analysis illustrates the role of PANoptosis-based subtypes in atherosclerosis and new diagnostic biomarkers
Authors: Chen HongKui , Xie Xianwei , Xiao Huazhen , Liang Wenjia , Lin Biting , Lin Zhijie , Lin KaiYan , Chen Chun , Guo Yansong 
Published: September 2023

Abstract
As a chronic inflammatory disease, atherogenesis and ischemia events are primarily affected by inflammation in atherosclerosis (AS). PANoptosis has been implicated in many human systemic disorders, including infection, cancer, neurodegeneration, and inflammation. On the other hand, little is understood about PANoptosis's function in atherosclerosis. The GSE100927 dataset has been divided using a consensus clustering approach into two PANoptosis-related subtypes based on the expression of PANoptosis-related genes (PRGs). We screened 36 PANoptosis-associated genes using the limma package and weighted gene co-expression network analysis (WGCNA), and then we enriched the PANoptosis-associated genes using ClueGO software. The PPI network was built using the STRING database and Cytoscape software, and the enriched genes were identified using LASSO regression. As atherosclerosis diagnostic markers, CCL3, ACP5, and HMOX1 have been identified and verified in the GSE43292 dataset. The variations in immune infiltration between AS and control samples and the gene clusters connected to PANoptosis were compared using the "CIBERSORT" package. The three diagnostic genes' expression levels were noticeably greater in the AS group. The three diagnostic genes were predominantly connected to macrophages, according to correlation analysis and single-cell analysis. The high expression of ACP5 and HMOX1 in RAW264.7 macrophages treated with OX-LDL was confirmed by PCR. Our study identified ACP5 and HMOX1 as diagnostic genes for AS that may be associated with PANoptosis.ACP5 and HMOX1 may be involved in the pathogenesis of AS by regulating macrophage PANoptosis.
Increased non-specific immune activity of vaname shrimp Litopenaeus vannamei using a leaf flour mixture from Ocimum basilicum and Piper betle and their characteristic compounds
Authors: Harlina Harlina , Rosmiati Rosmiati , Hamdillah Andi , Syahrul Syahrul , Isnansetyo Alim 
Published: September 2023

Abstract
Immunostimulants are more suitable for improving the health of shrimp than using drugs in the prevention of diseases. This study evaluated the efficacy of bioactive compounds from Ocimum basilicum and Piper betle in increasing the non-specific immune response of Litopenaeus vannamei. Samples were collected directly from a traditional market. Shrimp was fed: O. basilicum leaf flour- and P. betle leaf flour-un-supplemented diet (P1), O. basilicum leaf flour (44.84 g/kg feed)-supplemented diet (P2), P. betle leaf flour (31.57 g/kg feed)-supplemented diet (P3), and O. basilicum leaf flour (22.42 g/kg feed)- and P. betle leaf flour (16.79 g/kg feed)-supplemented diet (P4). Phytochemical screening tests were conducted using commonly used chemical reagents, including Dragendorff, Meyer, and Liebermann-Burchard reagents. Supplementation with O. basilicum and P. betle (P4) significantly enhanced the shrimp’s immune response, namely the total hemocyte counts (THC), phagocytic activity (PA), phagocytic index (PI), and prophenoloxidase (proPO). The supplemented diet (P4) was synergistically able to improve shrimp health, with the highest values for THC (7.30 x 106 cells/mL), hyaline (61.33%), PA (84%), PI (2.19), and proPO (0.91). Flavonoids and alkaloids in the feed containing leaf flour from both plants were bioactive compounds suspected to be responsible for enhancing the immune response. These findings suggest that a leaf flour combination of O. basilicum and P. betle can be a new immunostimulant alternative to improve shrimp health for disease prevention.
Network Intrusion Detection Empowered with Federated Machine Learning
Authors: Nasir Muhammad Umar , Mehmood Shahid , Khan Muhammad Adnan , Zubair Muhammad , Khan Faheem , Lee Youngmoon 
Published: September 2023

Abstract
Security and privacy are greatly enhanced by intrusion detection systems. Now, Machine Learning (ML) and Deep Learning (DL) with Intrusion Detection Systems (IDS) have seen great success due to their high levels of classification accuracy. Nevertheless, because data must be stored and communicated to a centralized server in these methods, the confidentiality features of the system may be threatened. This article proposes a blockchain-based Federated Learning (FL) approach to intrusion detection that maintains data privacy by training and inferring detection models locally. This approach improves the diversity of training data as models are trained on data from different sources. We employed the Scaled Conjugate Gradient Algorithm, Bayesian Regularization Algorithm, and Levenberg-Marquardt Algorithm for training our model. The training weights were then applied to the federated learning model. To maintain the security of the aggregation model, blockchain technology is used to store and exchange training models. We ran extensive testing on the Network Security Laboratory-Knowledge Discovery in Databases (NSL-KDD) data set to evaluate the efficacy of the proposed approach. According to simulation results, the proposed FL detection model achieved a higher accuracy level than the traditional centralized non-FL method. Classification accuracy achieved by the proposed model was 98.93% for training and 97.35% for testing.
Can carbon emission trading contribute the synergistic reduction of carbon and pollution emissions in China？A marginal abatement cost perspective
Authors: Li Mingwei , Mu Xianzhong , Xie Liang , Tian Zhiguang , Zeng Zhen , Hu Guangwen 
Published: September 2023

Abstract
Carbon emission trading policy, as a well-known emissions reduction strategy, can reduce both carbon and pollutant emissions. But it is still unclear whether and how much it can lower the marginal abatement costs of pollutants while reducing the marginal abatement costs of carbon. Studying the carbon emission trading policy’s level of savings on marginal abatement costs and the presence or absence of synergistic effects from a cost viewpoint are crucial in this context. This research evaluates the marginal abatement costs of carbon and pollutants in 30 Chinese provinces from 2000 to 2019. Then, a difference in difference (DID) model is used to evaluate the direct impact of carbon emission trading policy on marginal abatement costs, and its dynamic change properties are examined. The indirect impact of carbon emission trading policy on marginal abatement costs is next examined from the standpoint of abatement space and technological progress, and lastly the overall impact is taken into consideration. The findings indicate that: (i) The measured marginal abatement costs exhibits variations based on time and location. (ii) The carbon emission trading policy has a synergistic impact, lowering both the marginal abatement costs of pollutants and the marginal abatement costs of carbon, especially the former. (iii) The carbon emission trading policy tends to have a steadily diminishing effect on marginal abatement costs over time. (iv) The carbon emission trading policy not only reduce carbon and pollutant emissions, but also indirectly affect marginal abatement costs through technological progress and abatement space, and the indirect effect had a stronger impact on marginal abatement costs of pollutants compared to marginal abatement costs of carbon. Based on the study’s findings, policy proposals are put forth to encourage the cost-based synergy of pollution and carbon emissions reduction.
Assessment of Oxidative Stress in Zebrafish (Danio rerio) Exposed to Fenvalerate: Analysis of Antioxidant Enzyme Gene Expression and Enzyme Activity
Authors: Güneş Muharrem , Bayır Mehtap , Bayır Abdulkadir 
Published: September 2023

Abstract
This study aimed to investigate the effects of fenvalerate treatment on the expression of sod1, cat, gstt1a, gpx1a, gsr, and g6pd genes, as well as the activities of SOD, CAT, GST, GPX, GR, and G6PD enzymes in zebrafish. The assessment of oxidative stress was conducted by analyzing liver and gill tissue samples. Both the concentration of fenvalerate (3.125 and 6.25 µg/L) and the duration of exposure influenced the changes observed in antioxidant enzyme activities and gene expression in zebrafish. The RT-PCR results indicated that the expression of these genes in fenvalerate-exposed zebrafish might function as an immediate response mechanism to chemical-induced stress. Furthermore, the activities of CAT, GST, and G6PD enzymes in both tissues exhibited an increase exposure to fenvalerate doses of 3.125 and 6.25 µg/L. The activities of CAT, GST, and G6PD enzymes in both liver and gill tissues demonstrated a decrease. However, prolonged exposure (over 72 hours) resulted in significantly higher MDA concentrations in the liver and gill tissues compared to the control sample. These findings contribute to our understanding of the role of antioxidant enzymes in oxidative stress and have practical implications for designing aquaculture selection programs to enhance stress tolerance in fish.
Outcomes and Complications of Percutaneous Nephrolithotomy (PCNL) at Al-ribat Urology Center (Omer Sawi Hospital)
Authors: Jaber Mohammed Hammad , MOHAMMED HUSSAM ELDEIN HASSAN , AHMED HUSSAIN MOHAMMED , HASSN. HASSAN ISHAG , MAGBOL MUSTAFA ALTAYEB IBRAHIM , SAEED SAMI EL ARBAB 
Published: September 2023

Abstract
Objective: Percutaneous nephrolithotomy generally is safe, effective, and associated with a few but specific complications. However, the definition of complications of PCNL and their management still lacks consensus. We aimed to evaluate the Technique, Outcome, and Complication of percutaneous Nephrolithotomy at Al-ribat Urology Center (Omer Sawi Hospital).
methods :
A prospective, institutional-based cross-sectional study, the study investigated all patients presented to the study area with renal stones The data was collected by a pre-designed questionnaire filled out inside the theater during the surgery and 1-month follow-up by phone calls.
Result:
the study assessed the outcome of PCNL among 28 patients. The study found that the mean size of stones recorded was 1.25 cm, and the stones were on the left and right kidneys in 53.6%, and 46.4% respectively. Stones present by a percentage of 10.7%,28.6%, and 53.6% in the upper and lower calyx and pelvis respectively. The stones were rounded in 78.6% and staghorn in 21.4%. the stone was single in 53.6% and multiple in 46.4%. all operations were done under fluoroscopy guidance. Patients were supine in 25% and prone in 75% of the operations. Injury to the surrounding organs did not occur. Mild bleeding occurs in 10.7% of the patients, in which blood transfusion was necessary. Extravasation of fluid occurs in 10.7%. Fever occurs in 42.9%. Most of the patients stay for 1 day postoperatively 57.1%.
Conclusion: This study demonstrated that PCNL is a safe elective, minimally invasive surgical modality with good outcomes and lower incidences of major complications.
Infection Prevention Bundle in Preventing Implant-related Infections for Sacral Neuromodulation: A Single Surgeon Experience
Authors: Wu Liyang , Zhang Jianzhong , Zhang Peng 
Published: September 2023

Abstract
Background Implant-related infections (IRIs) after sacral neuromodulation (SNM) carry a high morbidity. Meanwhile, studies on care bundles report lower infection rate. Herein, we investigate the effectiveness of infection prevention bundle (IPB) in minimizing IRIs after SNM.
Methods An IPB focused on pre-operative checklists, weight-based antibiotic prophylaxis, strict draping and surgical techniques, and wound care education; was implemented in our center in February 2018. we performed a retrospective review of patients who underwent SNM-I testing from September 2013 to March 2023.
Subjects were divided into pre-IPB and post-IPB groups. All cases were performed by a single surgeon. Infection rates were calculated for pre-IPB and post-IPB groups.
Results A total of 110 patients underwent SNM-I testing during the study period. There were 28 pre-IPB and 82 post-IPB surgical cases. Only one patient in the pre-IPB group had IRI, while no infected patient in post-IPB group. Both groups had no significant differences in gender distribution, age, surgery duration, test period and the rate of infected patients.
Conclusions Although significantly prolonging the test stage，implementation of an IPB approach can be potentially decrease the incidence of IRIs.