bluuebunny/crossref_metadata_2025_split huggingface dataset:
Nipah Virus Infection Complicated with Encephalitis and Pneumonia Leading to Fatal Outcome: A Case Report from Bangladesh, January 2024
Authors: Chowdhury Tasmina , Urmee Israt Jahan , Sharna Jannaton Nisa , Momo Naushin Raisa , Chowdhury Mintu , Hossain M Sazzad , Shirin Tahmina , Rahman Md Mujibur 
Published: February 2025

Nipah virus (NiV) has been a major cause of encephalitis out-breaks with high mortality, primarily in the Indo-Bangladesh regions. Clinical presentations range from asymptomatic infection to acute respiratory infection and fatal encephalitis. Diagnosis can be confirmed by isolation from nucleic acid amplification in the acute phase or antibody detection during the convalescent phase. Treatment is mostly limited to supportive care and syndromic management of acute encephalitis syndrome. Here we discuss a case of middle-aged man from Manikganj district who presented with features of bilateral pneumonia and encephalitis. He progressed to rapid deterioration with development of type 2 respiratory failure of central origin and drop down of GCS to 7/15. Series of investigations revealed encephalitis but causative organism remained undetectable. Clinical suspicion carried out CSF IgM and IgG against NiV. Positive report and ultimate delayed revealed history of raw palm date sap consumption aided in confirmation of diagnosis. Patient was managed in ICU setting with mechanical ventilation, broad spectrum antibiotics, antiviral and steroid. Unfortunately, patient died on 28 January 2024 at infectious disease hospital.
J MEDICINE 2025; 26: 80-84
Pre-Hospital Care and Referral Pattern among Admitted Patients with Dengue Fever in Dhaka Medical College Hospital
Authors: Hoque Mohammad Mahfuzul , Kabir Md Shafiul , Biswas Sumit Kumar , Barshan Anindita , Mahboob Amirah , Rashid Rajilur , Tasneem Tahsina , Uzzaman Farid , Biswas Subrata Kumar , Afroze Naima , Hasan Atiqul , Datta Ponkaj Kanti , Mallik Md Uzzwal , Amin Mohammad Robed , Bari Shafiqul 
Published: February 2025

Introduction: Dengue has become a pressing public health concern for Bangladesh, with a significant rise in the death toll in recent years. Dhaka Medical College Hospital, the largest referral hospital, treats a large number of patients dengue patients. This study aims to observe prehospital treatment and referral patterns among patients admitted to the hospital with dengue fever.
Methods: This observational, cross-sectional study was conducted at Dhaka Medical College Hospital from September 2023 to November 2023, involving patients admitted with dengue infection. All consecutive patients with laboratory-confirmed dengue admitted to one unit (Medicine unit: 3) of the Department of Medicine were included. The data were analyzed using SPSS 25 software.
Results: Among the 488 patients, 273 (55.9%) were male. Most patients range in the age group 26-44 (39.5%). The Mean age of patients was 34.16±16.06. The majority, 160 (32.8%) and 123 (25.2%) were homemakers and students, respectively. Most patients (50%, n=244) were diagnosed with Dengue with warning signs (Category B). The mean hospital stay duration was 5.05±2.018 days, and the majority, 438 (89.8%), stayed in the hospital for ≤7 days. The mean ± SD of illness duration was 5.58 ±2.32 days. About 125 (25.6%) patients had co-morbid diseases, and hypertension was the most frequent comorbid condition. The majority of patients, 436 (89.3%) had no co-infections. Eighty-one (16.6%) and 79 (16.2%) patients were treated with antibiotics and papaya leaf extract juice or capsule before hospital admission, respectively, and a few patients used Eltrombopag (2.9%) as well. Among 488 patients, 201 (41.2%) patients were admitted by themselves. Approximately 479 (98.2%) patients survived and were discharged, and five patients died, all of them admitted to the hospital with severe dengue (Category C).
Conclusion: A significant portion of patients had practices using medication like antibiotics, steroids, papaya eltrombopag, etc., without indication of use. Self-hospitalisation without using a referral system was the most common form of hospitalization among patients with dengue fever.
J MEDICINE 2025; 26: 36-42
COMPUTER ASSISTED LANGUAGE LEARNING THROUGH ENGLISHCLASS101.COM: TO UNRAVEL THE UNDERPINNING METHODOLOGY
Authors: Maakh Yeri Nofita , Jonathans Peggy Magdalena 
Published: February 2025

English Language Teaching (ELT) is supposed to assist learners in actual life at the marketplace. Computer Assisted Language Learning (CALL) globally has flourished with tremendous products to address the purpose, e.g., EnglishClass101.com. Though this website includes a few aspects of ELT, the learning platform draws our attention for its affordability of price, feasibility of use, and recency of topics. The present study aims to unravel the underpinning teaching methodology of the website, with a particular focus on English grammar teaching. The present study employs a qualitative study design with two instruments of data collection: observation and transcription of lesson videos. The results indicate that contextual English grammar was used and integrated with lessons (newest and favorite); vocabulary (lists, words of the day, common words, key phrases); teacher assistance (explanation, assessment, tests). Majorly, two methods have been elaborated throughout the lessons on the website, i.e., Communicative Language Teaching, and Direct Method. Additionally, a few techniques are performed including teachers' gestures for meaning, annotation, repetition, drill, elicitation, option matching, deductive and inductive approach. The findings imply that any CALL products remain in use when the aspects of lessons involve daily topics and select feasible ELT methods
Adherence to phase I cardiac rehabilitation in post-PCI patients: a latent class analysis
Authors: Chai Zhuo , Fan Yuxuan , Gong Xue , Zhang Yue , Hu Yanling , Li Xiaowei , Fan Zhiqing , Han Yongkui 
Published: February 2025

BackgroundCoronary heart disease seriously jeopardizes human health and has become a principal public health problem of global concern. While percutaneous coronary intervention (PCI) repairs narrowed arteries and extends patients' lives, cardiac rehabilitation offers additional benefits post-PCI. Numerous previous studies have shown that cardiac rehabilitation can inhibit the progression of atherosclerotic plaques in patients after coronary intervention, effectively controlling patients' clinical symptoms and improving their quality of life. However, the current status of adherence to phase I cardiac rehabilitation is poor, and the variation in adherence to phase I cardiac rehabilitation among post-PCI patients in China are not well understood. This study aimed to identify the heterogeneity of adherence to phase I cardiac rehabilitation and its influencing factors in post-PCI patients through latent class analysis (LCA) to support individualized interventions.MethodsPCI patients (N = 212) admitted to the Cardiac Rehabilitation Center of Daqing Oilfield General Hospital in Heilongjiang Province were selected to complete the General Demographic Information Questionnaire, Cardiac Rehabilitation Adherence Scale for Coronary Heart Disease Patients, Cardiac Rehabilitation Knowledge Questionnaire, Patient Health Questionnaire-9 (PHQ-9), Social Support Rating Scale, Tampa Scale for Kinesiophobia-Swedish Version (TSK-SV), and Chronic Disease Resource Survey Questionnaire at the end of phase I cardiac rehabilitation. Latent class analysis identified potential categories of adherence to phase I cardiac rehabilitation in post-PCI patients. Logistic regression analyzed the factors influencing the different categories.ResultsAdherence to phase I cardiac rehabilitation in post-PCI patients was classified into three groups: Good Adherence (31.2%), Poor Nutritional and Psychological Management (32.0%), and Lack of Exercise (36.8%). Limited social support, poor utilization of chronic disease resources, low education level, a history of alcohol consumption, and kinesiophobia are factors influencing the different latent subgroups (P &lt; 0.05).ConclusionHeterogeneity exists in the adherence to phase I cardiac rehabilitation of post-PCI patients. Healthcare professionals should implement targeted interventions based on the characteristics of each category to improve adherence.
Comprehensive bioinformatics analysis of hub genes in ischemic heart failure and atrial fibrillation
Authors: Zhou Meimei , Xu Youkang , Zhang Lili , Yang Yushan , Zheng Jiejiao 
Published: February 2025

BackgroundAtrial fibrillation (AF) and heart failure (HF) frequently coexist and mutually influence each other. The association between AF and the subtype of HF, Ischaemic heart failure (IHF), remains insufficiently described, despite their high prevalence. Hence, comprehending their underlying pathophysiological mechanisms and identifying new therapeutic targets are urgently needed.ObjectiveThis exploration aims to unearth related genes and pathways of IHF and AF, offering new perspectives for their joint diagnosis and treatment.MethodsDatasets for HF (GSE57338) and AF (GSE128188) were acquired from the Gene Expression Omnibus (GEO) database. Intersecting these sets generated common differentially expressed genes (DEGs) for further analyses, including Gene Ontology (GO) enrichment, Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways, protein-protein interaction (PPI), and hub gene identification. Subsequently, the HF dataset (GSE116250) and AF dataset (GSE2240) were utilized to confirm the expression of the hub genes, followed by examination of gene expression patterns across cells in single-cell datasets.ResultsThe study identified 20 common DEGs. Among them, 10 hub genes (SFRP4, FMOD, HAPLN1, LTBP2, SVEP1, BCL6, ANPEP, CD38, ATRNL1, and BEX1) were found to be associated with the co-occurrence of IHF and AF. Enrichment analysis revealed the predominant involvement of these hub genes in extracellular matrix (ECM). Data from the Uniprot database revealed the involvement of the Wnt signaling pathway and TGF-β1/Smads signaling pathway in the development and progression of AF and IHF. Single-cell analysis demonstrated high gene expression primarily in monocytes.ConclusionThe identified 10 hub genes can serve as potentially valuable biomarkers for IHF and AF. Enrichment analysis reveals that these potential biomarkers are significantly associated with ECM, nicotinate, and nicotinamide metabolism, providing a foundational target for the joint diagnosis and treatment of the two diseases.
Flexural properties of rapidly prototyped denture base materials: the effect of nanoparticle addition and post-curing duration in vitro
Authors: Fouda Shaimaa M. , Gad Mohammed M. , Zayat Mai El , Khan Soban Q. , Akhtar Sultan , Othman Ahmed , von See Constantin 
Published: February 2025

ObjectivesThe flexural strength and elastic modulus of rapidly prototyped denture base materials are affected by numerous factors including reinforcement with nanoparticles (NPs) and post-curing duration (PCD), though the effect of these two factors together has been overlooked. The present study tested the effect of nanodiamonds (NDs) or silicon dioxide nanoparticles (SNPs) with various PCDs on the flexural strength and elastic modulus of rapidly prototyped denture base materials.MethodsTo measure the flexural strength and elastic modulus, bar-shaped specimens (64 × 10 × 3.3 mm) were designed and rapidly prototyped using ASIGA and NextDent denture base resins. Each resin (N = 150) was divided into five groups (n = 30) according to NP type and concentrations: pure group as a control without additives, 0.25% NDs, 0.5% NDs, 0.25% SNPs, and 0.5% SNPs. Specimens from each group were further divided into three groups (n = 10) and post-cured for 15, 60, or 90 min, followed by thermocycling for 5,000 cycles. After measuring the flexural strength and elastic modulus using a three-point bending test, a scanning electron microscope was used to analyze the fractured surface. The bonds between the NPs and the resin were tested by Fourier-transform infrared spectroscopy. ANOVA and post hoc tests were used for data analysis (α = 0.05).ResultsThe flexural strength increased with prolonged PCD and the highest values for all tested groups were reported at 90 min (P &lt; 0.001). The flexural strength of both materials increased significantly with the addition of NDs and SNPs in comparison to the pure groups (P &lt; 0.05). K-factor ANOVA analysis of the elastic modulus showed that each factor (NP type, PCD, and material type) had a significant effect on the elastic modulus (P &lt; 0.001).ConclusionThe flexural strength and elastic modulus of rapidly prototyped denture base resin were increased with the addition of NDs or SNPs and when increasing the PCD. Factors including nanoparticle type and concentration, the post-curing duration, and the material type solely or in combination could affect the flexural strength and elastic modulus of prototyped denture base materials.
“COVID-19 affected me greatly (sigh), imagine I'm being called a mother and yet I'm also a child”: the effect of COVID-19 on fertility management practices among women in Nairobi and Kisumu cities, Kenya
Authors: Kwena Zachary Arochi , Wekesa Pauline , Gitome Serah , Okumu Sarah , Ndunyu Louisa , Bukusi Elizabeth , Himes Emily , Holt Kelsey , Liu Jenny , Vallin Janelli , Suchman Lauren 
Published: February 2025

BackgroundThe COVID-19 pandemic strained the provision of sexual and reproductive health services, including family planning, which were categorized as non-essential services at the peak of COVID-19 infection control in Kenya. We set out to assess the effect of COVID-19 on fertility management practices among Kenyan women in two cities to inform mitigation measures in future similar disruptions.MethodsThis was a qualitative study to describe the effect of the COVID-19 pandemic on women's fertility management practices from 61 in-depth interviews (IDIs) with women aged 15–45 years residing in Nairobi and Kisumu, Kenya, between February and May 2021. Identified participants were consented and interviewed at convenient locations. We used a constant comparative analysis that compared emergent themes across topics and transcripts to identify and group those that are similar or dissimilar to arrive at insights that informed our conclusions.ResultsOur findings show that COVID-19 affected women's fertility management practices at individual, interpersonal, and organizational levels. At the individual level, lack of money due to COVID-19-induced economic difficulties made family planning services unaffordable to women. Other women delayed their conception plans until their financial situation improved. At the interpersonal level, travel restrictions separated couples, making it challenging for those who had plans to conceive to actualize their fertility plans. Additionally, women who reported to be sexually inactive relaxed adherence to their contraceptive use schedule because of the reduced risk of unintended pregnancy. Finally, at the organizational level, provider shortages, long queues, and contraceptive stockouts during COVID-19 compromised women's access to family planning services. We also found that a minority of women who were either not using contraceptives or who were on long-acting methods perceived little or no effect of COVID-19 on their fertility management practices.ConclusionCOVID-19's effect on women's fertility management practices manifested at individual, interpersonal, and organizational levels. There is a need to devise strategies that empower women to deal with their family planning needs and those that make healthcare systems resilient enough to handle the effects of emergent crises without compromising the provision of existing services.
Treatment at the end of life in patients with advanced melanoma. A multicenter DeCOG study of 1067 patients from the prospective skin cancer registry ADOReg
Authors: Forschner Andrea , Kähler Katharina C. , Gschnell Martin , Langan Ewan A. , Weishaupt Carsten , Meiss Frank , Thoms Kai-Martin , Wahl Renate U. , Göppner Daniela , Garzarolli Marlene , Sachse Michael , Schlaak Max , Reitmajer Markus , Kellner Ivonne , Gesierich Anja , Mohr Peter , Meier Friedegund , von Wasielewski Imke , Herbst Rudolf , Utikal Jochen , Pföhler Claudia , Ulrich Jens , Terheyden Patrick , Kaatz Martin , Haferkamp Sebastian , Leiter Ulrike , Ugurel Selma , Weichenthal Michael , Berking Carola , Gutzmer Ralf , Schadendorf Dirk , Nanz Lena , Loquai Carmen 
Published: February 2025

BackgroundAlthough systemic therapies have improved considerably over the last decade, up to 50% of patients with metastatic melanoma still die due to disease progression. Oncological treatment at the end-of-life phase is challenging. The aim of this study was to investigate the frequency and type of systemic therapy received by melanoma patients in their end-of-life phase.MethodsPatients with metastatic melanoma who had died between January 1, 2018 and October 31, 2022 were identified from the prospective multicenter skin cancer registry ADOReg. Study endpoints were percentage of patients who had been treated with systemic therapy within the last three months of life, timepoint of initiation of the last-line therapy, overall survival, treatment benefit and the incidence of treatment-related adverse events.ResultsIn total, 1067 patients from 46 skin cancer centers were included. Most of the patients (63%) had received immune checkpoint inhibitors (ICI) as last-line therapy, 22% targeted therapies (TT) and 12% chemotherapy (CTX). Comparing last-line ICI and TT, patients with TT were significantly more likely to benefit from treatment and had significantly fewer and milder treatment-related AE than patients with ICI. Even though two thirds of patients had received ICI as a last-line therapy, the majority of these patients (61%) had stopped therapy within the last 30 days of life, whereas the majority of patients with TT (66%) still continued their treatment to the end of life. We found markedly fewer patients with initiation of ICI within 30 days before their death (19%) compared to a historic cohort including patients who died in 2016 or 2017 (39%).ConclusionTreatment approaches near the end of life have markedly changed in skin cancer centers in Germany over recent years, with ICI prescribed less frequently in the end-of-life phase. In contrast, TT are frequently administered, even within the last 30 days of life. It should also be considered that discontinuation of TT can result in rapid tumor progression. Due to the oral administration and a low rate of severe toxicity, TT appear to be a suitable treatment option, even in the end-of-life situation of melanoma patients.
Allelic variations in putative antimalarial drug resistance and vaccine candidate genes among Plasmodium vivax reference genomes
Authors: Tamang Suman , Arya Aditi , Singh Vineeta 
Published: February 2025

ObjectivesPlasmodium vivax is the most widespread Plasmodium spp. globally and the second most common cause of human malaria. However, very little is known about its biology due to the lack of continuous in vitro/ex vivo culture. In such circumstances, genomic studies provide an alternative for understanding the biology of P. vivax parasites. To date, most of the genomic studies on P. vivax have been based on Sal-I as a reference genome; however, with the recent release of the PvP01 and PvW1 reference genomes with higher quality and improved assemblies following continual improvements in annotation, the adoption of these genomes as a reference for genomic studies appears more advantageous. In this study, allelic differences in putative antimalarial drug resistance genes and vaccine candidate genes with assembly features of these three genomes are analyzed and summarized.MethodsThe nucleotide and amino acid sequences of five P. vivax putative antimalarial drug resistance genes and three vaccine candidate genes for all three reference genomes (i.e., Sal-I, PvP01, and PvW1) were retrieved from PlasmoDB and aligned together using MEGA11. Any differences in the nucleotide and codon sequences between the genomes were recorded.ResultsVarious allelic differences in the putative antimalarial drug resistance and vaccine candidate genes between the three reference genomes were observed, which included the positions previously identified as candidate markers for these gene variants.ConclusionsAs antimalarial drug resistance genotyping studies rely on candidate genetic markers to classify resistant or sensitive parasites, knowledge of the allelic differences among references is important. Furthermore, the heterogeneity of the vaccine candidate genes should be taken into account when designing vaccines for P. vivax.
Effects of copper and copper oxide nanoparticles on cyanobacterium Nostoc linckia: an experimental study
Authors: Cepoi Liliana , Rudi Ludmila , Chiriac Tatiana , Valuta Ana , Codreanu Svetlana , Mitina Tatiana , Codreanu Liviu 
Published: February 2025

IntroductionCopper nanoparticles (CuNPs) and copper oxide nanoparticles (CuONPs) are increasingly explored for their biological interactions with various organisms, including cyanobacteria, due to their unique properties and potential applications. This study investigates the effects of CuNPs and CuONPs on the cyanobacterium Nostoc linckia (Roth) Born et Flah CNMN-CB-03, focusing on biomass accumulation, biochemical content, pigment composition, and microscopic structural changes.MethodsNostoc linckia cultures were exposed to CuNPs and CuONPs at concentrations ranging from 0.1 to 30 mg/L. The impact on biomass, protein, pigment, lipid content, malondialdehyde (MDA) levels, and bioaccumulation of copper was assessed, alongside microscopic analysis to observe any structural modifications in trichomes.ResultsThe effects of CuNPs and CuONPs on Nostoc linckia were distinct. Under high concentrations of CuNPs exposure, reductions in biomass, protein content, and pigments were observed, whereas lipid and MDA content increased significantly. Similarly, CuONPs caused a marked increase in lipid and MDA levels, suggesting oxidative stress despite the comparatively moderate alterations in other biochemical parameters. Both nanoparticle types, however, caused notable bioaccumulation of copper and structural modification in Nostoc linckia cells expressed in trichome fragmentation, chromaticity changes, and variations in heterocyst numbers and size in treated samples.ConclusionCuNPs and CuONPs exhibit differential effects on Nostoc linckia, influencing biochemical composition, pigment profiles, and cellular structure. These findings contribute to understanding nanoparticle interactions with cyanobacteria and highlight the distinct impact of nanoparticle composition on microbial systems.