bluuebunny/crossref_metadata_2025_split huggingface dataset:
177Lu-FAP-2286 Therapy in a Case of Recurrent Bladder Cancer With Multiple Metastatic Lesions
Authors: Li Linwei , Yang Jian , Peng Dengsai , Zhang Yu , Chen Yue 
Published: September 2023

Abstract
We reported a 73-year-old man who was diagnosed with the recurrence of bladder tumor received 177Lu-FAP-2286 treatment. Encouragingly, radiological remission and alleviation of his symptom were noted. In addition, the patient did not have any adverse effects.
Papillary Thyroid Cancer in Thyroglossal Duct Cysts
Authors: Bishop Grace L. , Jasim Sina , Strnad Benjamin S. , Itani Malak 
Published: September 2023

Abstract
Thyroglossal duct cyst is the most common congenital neck mass, but the incidence of malignancy within a thyroglossal duct cyst is rare, estimated at 1%. Most cancers arising within thyroglossal duct cysts are incidentally detected after surgical excision. We present the preoperative radiologic findings of 8 patients with papillary thyroid cancer arising within a thyroglossal duct cyst, as evaluated on ultrasound, computed tomography, magnetic resonance imaging, and positron emission tomography scan.
Handheld Doppler Detection and Light Illumination for Vascular Mapping in Nasal Reconstruction
Authors: Zhang Xulong , Song Zhen , Xu Yihao , Zheng Ruobing , Guo Junsheng , Tian Le , Wang Huan , You Jianjun , Fan Fei 
Published: September 2023

Background:
The application of the expanded forehead flap in nasal reconstruction has the advantage of being able to provide a sufficient amount of flap and can provide good aesthetic results. For an expanded forehead flap to survive, there must be adequate arterial supply and venous return. Despite this, limited studies have been conducted on preoperative vascular mapping and the design of the expanded forehead flap for nasal reconstruction. In this article, the authors present a technique of hand-held Doppler detection with light illumination for vascular mapping.

Patients and Methods:
The study included patients who underwent total nasal reconstruction with expanded forehead flaps between May 2016 and April 2021. The design of the flap was based on the result of preoperative vascular detection by hand-held Doppler detection assisted by light illumination.

Results:
A total of 32 patients underwent total nasal reconstruction with an expanded forehead flap. The distal part of the flap became necrotic 1 week after the surgery in 2 patients. Following dressing changes and the administration of antibiotics, the distal flap in these patients survived well. No complications were reported in the long term.

Conclusions:
Hand-held Doppler detection combined with light illumination is a convenient and effective preoperative design method for nasal reconstruction with an expanded forehead flap. All flaps survived well in the long term.

Level of Evidence:
Level IV.
Meta-Analysis of Validated Quality of Life Outcomes Following Voice Feminization in Transwomen
Authors: Hao Yvonne , Trilles Jorge , Brydges Hilliard T. , Boczar Daniel , Kurian Keerthi K. , Chaya Bachar F. , Colon Ricardo Rodriguez , Parker Augustus , Kwak Paul E. , Rodriguez Eduardo D. 
Published: September 2023

Objectives:
For transwomen undergoing voice feminization interventions, fundamental frequency (F0; vocal pitch) is a commonly reported functional outcome measure in the literature. However, F0 may not correlate well with improvement in quality of life (QoL). Several validated voice-related QoL instruments have been used to assess QoL improvement in these patients, yet there is no consensus on the most appropriate instrument. This systematic review and meta-analysis aimed to assess the relationship between change in F0 and QoL improvement following voice feminization, and to compare validated QoL instruments commonly used in this population.

Data Sources:
PubMed, Cochrane, and Embase.

Review Methods:
A systematic review was conducted following Preferred Reporting Items for Systematic Reviews and Meta-Analyses guidelines. Primary studies of transwomen undergoing voice feminization, reporting validated QoL outcomes were included. Meta-analyses for associations between mean change in QoL score and mean change in F0, as well as variations in mean change in QoL score by QoL instrument, were performed using a multilevel mixed effects model.

Results:
No statistically significant correlation was found between change in F0 and QoL score improvement post-intervention. Different validated instruments showed statistically significant variation in QoL score change, with the Trans Women Voice Questionnaire (TWVQ) capturing a greater improvement in QoL score relative to other instruments.

Conclusions:
Lack of correlation between changes in F0 and QoL improvement further supports that F0 alone is insufficient to assess the efficacy of voice feminizing interventions. Validated QoL measures are useful adjuncts. Of these, the TWVQ appears to be the most sensitive for measurement of QoL improvement following voice feminization.
Evaluation of Quality of Life and Self-reported Complications in Patients With Clean Intermittent Catheterization
Authors: Santos-Pérez de la Blanca Rocío , Medina-Polo José , González-Padilla Daniel , Cano-Galán María Mercedes , Arrébola-Pajares Ana , Hernández-Arroyo Mario , Rodríguez-Antolín Alfredo 
Published: September 2023

PURPOSE:
This study sought to determine health-related quality of life and self-reported complications associated with clean intermittent catheterization (CIC).

DESIGN:
Observational, cross-sectional study.

SUBJECTS AND SETTING:
The target population was patients cared for by the urology department at Hospital 12 de Octubre in Madrid, Spain, undergoing CIC for chronic urinary retention of any etiology (neurogenic bladder dysfunction, neobladder, and other). The sample comprised 50 respondents with a mean age of 49 years; a majority (66%, n = 33) were female. Participants performed an average of 4 CICs.

METHODS:
All participants completed the ISC-Q (Intermittent Self-Catheterization Questionnaire) and a questionnaire about CIC-associated complications. Data were collected in February 2019.

RESULTS:
A vast majority of respondents (98%, n = 49) indicated preparation for catheterization was simple, and 76% (n = 38) indicated the catheter was easy to insert. One in 5 (20%, n = 10) considered carrying catheters and supplies inconvenient, though 58% (n = 29) indicated it was easy to dispose of the catheters outside the home. Most respondents (98%, n = 49) indicated they felt self-conscious about their need to self-catheterize, and 16% (n = 8) felt that CIC created limitations when visiting friends and family. The most frequent complication was symptomatic urinary tract infections (UTIs); participants reported an average of 1.7 UTIs in the last year. Additional complications, such as epididymo-orchitis, urethral stenosis, and urethral bleeding, were reported by less than 5% (n = 2) of participants.

CONCLUSIONS:
Participants managed by CIC for chronic urinary retention of any cause reported acceptable levels of satisfaction with the procedure. The reported incidence of complications was low, except for UTIs.
Does Resilience Predict Ostomy Adjustment in Patients With Permanent Ostomy?
Authors: Tirgari Batool , Azizzadeh Forouzi Mansooreh , Heidarzadeh Azam , Khalilzadeh Ganjalikhani Mahboobeh 
Published: September 2023

PURPOSE:This study aimed to evaluate associations between resilience and ostomy adjustment in a group of patients with a permanent ostomy.DESIGN:Descriptive, correlational study.SUBJECTS AND SETTING:One hundred participants were interviewed; most were male (N = 56; 56%), married (N = 77; 77%), illiterate (N = 46; 46%), and self-employed (N = 35; 35%). The most frequent condition leading to ostomy surgery was colorectal cancer (N = 24; 24%). A majority (N = 43; 43%) had been diagnosed with cancer 1 to 5 years before data collection, and 35 (35%) had lived with an ostomy for 1 to 5 years. Participants were recruited from an ostomy clinic of in Kerman, located in southeastern Iran. Data were collected from August to October 2019.METHODS:Data were collected via interviews. A questionnaire, developed for purposes of this study, was developed that included items querying demographic and pertinent clinical characteristics and 2 validated instruments, the Resilience Scale and the Ostomy Adjustment Inventory-23 (OAI-23). Data were analyzed usingttests, analysis of variance, Pearson correlation coefficient, and linear regression.RESULTS:The mean score for ostomy adjustment was 43.37 (SD = 11.57) indicating less than optimal adjustment to life with an ostomy. The mean score of the Resilience Scale was 104.56 (SD = 25.98); most participants (n = 67, 67%) had very low and low resilience. Resilience was weakly associated with ostomy adjustment; specifically, participants who scored higher on the Resilience Scale tended to score higher on the OAI-23 (r= 0.32,P= .006). Linear regression indicated that resilience had the greatest predictive effect on ostomy adjustment (P= .004).CONCLUSIONS:Results suggest that higher levels of resilience may facilitate adjustment to a permanent ostomy. Findings indicate that nurses should design and implement programs to aid ostomy adjustment via resilience assessment.
Health Belief Scale for Urinary Incontinence and Kegel Exercise
Authors: Aydin Avci I˙lknur , Öz Yildirim Özge 
Published: September 2023

PURPOSE:
The aim of this study was to develop an instrument based on the Health Belief Model that measures urinary incontinence awareness and beliefs regarding pelvic floor muscle (Kegel) exercises.

DESIGN:
Evaluation of validity and reliability of the novel instrument and analysis of sociodemographic variables based on responses to this 49-item scale.

SUBJECTS AND SETTING:
The sample used to validate the instrument (n = 527) was drawn from patients cared for at the gynecology and obstetrics and urology polyclinic at Ondokuz Mayıs University Hospital. A majority of participants were female (n = 431, 81.8%) and their average age was 33.8 years (SD 12.3). The university is located in Samsun, a city located in northern Turkey. Data were collected between October 2019 and December 2020.

METHODS:
The Health Belief Scale for Urinary Incontinence and Kegel Exercise's validity was analyzed using a content validity index approach with 8 experts. Psychometric measurements were obtained using exploratory factor analysis and Cronbach's α reliability analysis. Exploratory factor analysis incorporated principal component analysis and varimax rotation. t Tests and 1-way analysis of variance were used to compare subdimensions of the Health Belief Scale for Urinary Incontinence and Kegel Exercise with some variables (sex, education status, marital status, make Kegel exercises regularly, etc).

RESULTS:
Results supported a scale having 49 items and 6 subscales per factor analysis explaining 59.8% of total variance. With exploratory factor analysis, the Kaiser-Meyer-Olkin coefficient was 0.899 and Barlett test result was χ² = 18389.424 (P = .001). Cronbach's α coefficients for the scale varied between 0.79 and 0.94. Factor loadings per underlying dimensions ranged from 0.42 to 0.84.

CONCLUSION:
The Health Belief Scale for Urinary Incontinence and Kegel Exercise is a valid and reliable instrument.
Enhancing Self-Management Skills of Patients With Existing Diabetic Foot Ulcerations
Authors: Zima Janice , Jairath Nalini 
Published: September 2023

PURPOSE:
The purpose of this quality improvement initiative was to determine the impact of a nurse-administered foot care intervention bundle (NA-FCIB) upon self-management knowledge, skills, and outcomes in patients with diabetic foot ulcerations.

PARTICIPANTS AND SETTING:
The sample comprised 39 patients being treated for diabetic foot ulceration at a wound care clinic in a tertiary care hospital in Arlington, Virginia. The project was conducted from August 2017 to February 2018.

APPROACH:
This quality improvement project used the Johns Hopkins Plan-Do-Study-Act Method supplemented by self-regulation theory for diabetic patient education and evidence in clinical literature. The 12-week-long intervention included one-on-one teaching in the prevention of ulcerations and optimal care of the diabetic foot, blood glucose level tracking logs, patient “teach-back” and skills demonstration, and free foot care tools.

OUTCOMES:
From baseline to post-NA-FCIB, the number of participants knowing the reasons for temperature foot protection increased by 92%, those knowing major factors leading to diabetic foot ulceration by 85%, those knowing what to look for in the foot self-exam by 85%, and those able to demonstrate correct foot self-exam by 84%. The number of participants understanding proper footwear increased by 74%, and those identifying ways to avoid/decrease the likelihood of diabetic foot ulcers by 72%. Mean serum hemoglobin A1c (HgbA1c) levels decreased from baseline to postintervention (8.27%; SD 2.05% vs 7.46%; SD 1.58%; P = .002).

IMPLICATIONS FOR PRACTICE:
The NA-FCIB intervention was successfully incorporated into routine clinic care as the standard of care. Our experience suggests that the NA-FCIB may be feasible and effective for use at comparable wound care clinics and may have secondary benefits for HgbA1c regulation.
Whole Genome Sequencing Based Analysis of Inflammation Biomarkers in the Trans-Omics for Precision Medicine (TOPMed) Consortium
Authors: Jiang Min-Zhi , Gaynor Sheila M. , Li Xihao , Van Buren Eric , Stilp Adrienne , Buth Erin , Wang Fei Fei , Manansala Regina , Gogarten Stephanie M. , Li Zilin , Polfus Linda M. , Salimi Shabnam , Bis Joshua C. , Pankratz Nathan , Yanek Lisa R. , Durda Peter , Tracy Russell P. , Rich Stephen S. , Rotter Jerome I. , Mitchell Braxton D. , Lewis Joshua P. , Psaty Bruce M. , Pratte Katherine A. , Silverman Edwin K. , Kaplan Robert C. , Avery Christy , North Kari , Mathias Rasika A. , Faraday Nauder , Lin Honghuang , Wang Biqi , Carson April P. , Norwood Arnita F. , Gibbs Richard A. , Kooperberg Charles , Lundin Jessica , Peters Ulrike , Dupuis Josée , Hou Lifang , Fornage Myriam , Benjamin Emelia J. , Reiner Alexander P. , Bowler Russell P. , Lin Xihong , Auer Paul L. , Raffield Laura M. ,   NHLBI Trans-Omics for Precision Medicine (TOPMed) Consortium,   TOPMed Inflammation Working Group
Published: September 2023

AbstractInflammation biomarkers can provide valuable insight into the role of inflammatory processes in many diseases and conditions. Sequencing based analyses of such biomarkers can also serve as an exemplar of the genetic architecture of quantitative traits. To evaluate the biological insight, which can be provided by a multi-ancestry, whole-genome based association study, we performed a comprehensive analysis of 21 inflammation biomarkers from up to 38,465 individuals with whole-genome sequencing from the Trans-Omics for Precision Medicine (TOPMed) program. We identified 22 distinct single-variant associations across 6 traits – E-selectin, intercellular adhesion molecule 1, interleukin-6, lipoprotein-associated phospholipase A2 activity and mass, and P-selectin – that remained significant after conditioning on previously identified associations for these inflammatory biomarkers. We further expanded upon known biomarker associations by pairing the single-variant analysis with a rare variant set-based analysis that further identified 19 significant rare variant set-based associations with 5 traits. These signals were distinct from both significant single variant association signals within TOPMed and genetic signals observed in prior studies, demonstrating the complementary value of performing both single and rare variant analyses when analyzing quantitative traits. We also confirm several previously reported signals from semi-quantitative proteomics platforms. Many of these signals demonstrate the extensive allelic heterogeneity and ancestry-differentiated variant-trait associations common for inflammation biomarkers, a characteristic we hypothesize will be increasingly observed with well-powered, large-scale analyses of complex traits.
Protein generation with evolutionary diffusion: sequence is all you need
Authors: Alamdari Sarah , Thakkar Nitya , van den Berg Rianne , Tenenholtz Neil , Strome Robert , Moses Alan M. , Lu Alex X. , Fusi Nicolò , Amini Ava P. , Yang Kevin K. 
Published: September 2023

AbstractDeep generative models are increasingly powerful tools for thein silicodesign of novel proteins. Recently, a family of generative models called diffusion models has demonstrated the ability to generate biologically plausible proteins that are dissimilar to any actual proteins seen in nature, enabling unprecedented capability and control inde novoprotein design. However, current state-of-the-art diffusion models generate protein structures, which limits the scope of their training data and restricts generations to a small and biased subset of protein design space. Here, we introduce a general-purpose diffusion framework, EvoDiff, that combines evolutionary-scale data with the distinct conditioning capabilities of diffusion models for controllable protein generation in sequence space. EvoDiff generates high-fidelity, diverse, and structurally-plausible proteins that cover natural sequence and functional space. We show experimentally that EvoDiff generations express, fold, and exhibit expected secondary structure elements. Critically, EvoDiff can generate proteins inaccessible to structure-based models, such as those with disordered regions, while maintaining the ability to design scaffolds for functional structural motifs. We validate the universality of our sequence-based formulation by experimentally characterizing intrinsically-disordered mitochondrial targeting signals, metal-binding proteins, and protein binders designed using EvoDiff. We envision that EvoDiff will expand capabilities in protein engineering beyond the structure-function paradigm toward programmable, sequence-first design.
The Effect of Arm Restriction on Dynamic Stability and Upper Body Responses to Lateral Loss of Balance During Walking: An Observational Study
Authors: Rosenblum Uri , Lavi Adi , Fischer Arielle , Parmet Yisrael , Haim Amir , Handelzalts Shirley 
Published: September 2023

AbstractWhen losing balance, upper-body movements serve as mechanical aids to regain stability. However, it remains unclear how these movements contribute to dynamic stability during recovery from a lateral loss of balance while walking with arm restriction. We aimed to 1) quantify the effect of arm restriction on gait stability and upper-body velocities, and 2) characterize upper-body kinematic strategies in response to lateral surface translations under different arm restriction conditions. Healthy adults were exposed to lateral surface translations while walking on a computerized treadmill under three conditions: ‘free arms’, ‘1-arm restricted’ and, ‘2-arms restricted’. Dynamic stability and upper-body velocities for the first step after perturbation onset were extracted. We found decreased dynamic stability in the sagittal plane and increased trunk velocity in the ‘2-arm restricted’ condition compared to the ‘free arms’ condition. Head and trunk movements in the mediolateral plane were in opposite directions in 44.31% of responses. Additionally, significant trunk velocities were observed in the opposite direction to the perturbation-induced loss of balance. Our results support the contribution of increased upper-body velocities to balance responses following arm-restricted walking perturbations and suggest that the ‘2-arm restricted’ condition may be utilized as a perturbation-based balance training, focusing on head and trunk responses.
Honey bees perform fine-scale detailing that continuously reduces comb area after nest expansion
Authors: Bailey Claire S. , Marting Peter R. , Smith Michael L. 
Published: September 2023

AbstractHoney bees are renowned architects. The workers use expensive wax secretions to build their nests, which reach a mature, seemingly steady state, relatively quickly. After nest expansion is complete, workers do not tear down combs completely and begin anew, but there is the possibility they may make subtle changes like adding, removing, and repositioning existing wax. Previous work has focused on nest initiation and nest expansion, but here we focus on mature nests that have reached a steady-state. To investigate subtle changes to comb shape over time, we tracked six colonies from nest initiation through maturity (211 days), photographing their combs every 1-2 weeks. By aligning comb images over time, we show that workers continuously remove wax from the comb edges, thereby reducing total nest area over time. All six colonies trimmed comb edges, and 98.3% of combs were reduced (n = 59). Comb reduction began once workers stopped expanding their nests and continued throughout the experiment. The extent to which a comb was reduced did not correlate with its position within the nest, comb perimeter, or comb area. It is possible that workers use this removed wax as a reserve wax source, though this remains untested. These results show that the superorganism nest is not static; workers are constantly interacting with their nest, and altering it, even after nest expansion is complete.
Uncoupling the TFIIH Core and Kinase Modules Leads To Misregulated RNA Polymerase II CTD Serine 5 Phosphorylation
Authors: Giordano Gabriela , Buratowski Robin , Jeronimo Célia , Poitras Christian , Robert François , Buratowski Stephen 
Published: September 2023

AbstractTFIIH is an essential transcription initiation factor for RNA polymerase II (RNApII). This multi-subunit complex comprises two modules that are physically linked by the subunit Tfb3 (MAT1 in metazoans). The TFIIH Core Module, with two DNA-dependent ATPases and several additional subunits, promotes DNA unwinding. The TFIIH Kinase Module phosphorylates Serine 5 of the C-terminal domain (CTD) of RNApII subunit Rpb1, a modification that coordinates exchange of initiation and early elongation factors. While it is not obvious why these two disparate activities are bundled into one factor, the connection may provide temporal coordination during early initiation. Here we show that Tfb3 can be split into two parts to uncouple the TFIIH modules. The resulting cells grow slower than normal, but are viable. Chromatin immunoprecipitation of the split TFIIH shows that the Core Module, but not the Kinase, is properly recruited to promoters. Instead of the normal promoter-proximal peak, high CTD Serine 5 phosphorylation is seen throughout transcribed regions. Therefore, coupling the TFIIH modules is necessary to localize and limit CTD kinase activity to early stages of transcription. These results are consistent with the idea that the two TFIIH modules began as independent functional entities that became connected by Tfb3 during early eukaryotic evolution.Significance StatementThe RNA polymerase II initiation factor TFIIH provides two activities needed for efficient transcription: promoter unwinding and phosphorylation of polymerase subunit Rpb1 on its C-terminal domain (CTD). This study shows these two functions can be physically uncoupled. Unexpectedly, when the CTD kinase is no longer recruited to the promoter, the CTD phosphorylation on Serine 5 is not lost, but instead occurs throughout transcription. These results suggest that fusion of the two TFIIH activities evolved to better target CTD Serine 5 phosphorylation to early elongation.