bluuebunny/crossref_metadata_2025_split huggingface dataset:
Occurrence of Cryptosporidium in human stool samples in Qatar
Authors: Sabooni Sallama , Salah Husam , Singh Rajvir , Al-Qadi Khloud , Taj-Aldeen Saad J. , Ibrahim Emad 
Published: August 2023

Abstract
Background Cryptosporidium is a common pathogenic parasite known to cause diarrhea in humans, particularly young children living in poor-resource settings, as well as animals. Symptoms are usually mild in immunocompetent individuals and may progress to a life-threatening disease among immunocompromised patients. The diagnosis of cryptosporidiosis can be challenging due to insensitive conventional diagnostic tests. This study aimed to investigate the prevalence of cryptosporidiosis infection in Qatar and to compare four different diagnostic methods for detection of Cryptosporidium in human stool samples.Methods Stool samples obtained from patients with various gastrointestinal symptoms were received at the microbiology laboratory of Hamad general hospital, Qatar, for ova and parasites examination over a period of two years (January 2018 to December 2019). Stool samples were tested using four diagnostic methods: routine microscopy, immunochromatography (ICT), multiplex polymerase chain reaction (PCR), and modified Kinyoun's acid fast stain (MKS).Results Out of 205 stool samples, we detected Cryptosporidium in 17.6%, 15.0%, 7.0%, and 6.0% of specimens using PCR, ICT, MKS, and routine microscopy, respectively. Among the 36 positive patients, 39.0⁒ patients were under five years old, 17.0⁒ were aged between five and 10 years, 19.0⁒ were between 10 and 20 years, 17.0⁒ were between 20 and 40, and 8.0⁒ were over 40 years old. OF the positive cases, 61.1⁒ patients were male and 38.9⁒ were female. Additionally, 61.1⁒ of patients were Qatari nationals, while 38.9⁒ were from other origins.Conclusions For a better diagnosis of Cryptosporidium infection, PCR or ICT techniques should be incorporated alongside conventional microscopy methods. Future research using multi-locus sequence typing will to provide valuable insight to the molecular epidemiology and species diversity of Cryptosporidium species in Qatar.
Fintech, Green Imports, Technology, and FDI Inflow: Their Role in CO2 Emissions Reduction and the Path to COP26 - A Comparative Analysis of China
Authors: Guo Qi , Yin Chengyuan 
Published: August 2023

Abstract
Using a Nonlinear Autoregressive Distributed Lag (NARDL) model, this study investigates the relationships among CO2 emissions, green energy imports, foreign direct investment (FDI) inflow, and financial technology (Fintech) in China. The study considers both short- and long-term asymmetries, reflecting both the positive and negative effects of the variables of interests on CO2 emissions. Results reveal that both green technology and fintech significantly increase CO2 emissions for both positive and negative shocks. In contrast, FDI inflows have conflicting outcomes, being positively beneficial during positive shocks and adversely significant during negative shocks. Furthermore, green energy imports result in a considerable rise in CO2 emissions during negative shocks. These findings emphasize the necessity of taking economic factors into consideration when developing environmental regulations. Under COP26 aims to build a greener, more sustainable future for China, policy recommendations include fostering sustainable Fintech innovation, investing in green technology research, bolstering renewable energy imports, and improving climate legislation.
Intention to Use Personal Health Record System and Its Predictors Among Chronic Patients Enrolled at Public Hospitals in Bahir Dar City, Northwest Ethiopia: Using Modified UTAUT-2 Model
Authors: Abebe Solomon Abuhay , Endehabtu Berhanu Fikadie , Walle Agmasie Damtew , Hailu Debela Tsegaye , Gashu Kassahun Dessie 
Published: August 2023

Abstract
Introduction: Chronic diseases are the leading causes of death in the world. In sub-Saharan Africa, it leads to more mortality than almost every other region in the world. Currently, digital health technology like personal health records plays a crucial role in managing patients with chronic diseases. In low-resource countries like Ethiopia, it is uncertain how many chronic patients intend to use PHRs and the accompanying circumstances. Hence, the aim of this study was to assess chronic patients’ intention to use PHRs and its predictors enrolled in public health hospitals in Bahir Dar City, northwest Ethiopia.
Method: An institutional-based cross-sectional study was conducted among 924 respondents from April 5 to May 9, 2023, in Bahir-Dar city public hospitals. A stratified sampling technique followed by a systematic sampling technique was applied to select the study participants. An interviewer-administrated questionnaire was conducted using Kobo Collect. A UTAUT2 model was applied to develop theoretical frameworks. SPSS version 25 software was used to estimate the descriptive statistics, and the structural equation model analysis was used to evaluate model constructs using AMOS version 21 software.
Results: In this study, a total of 908 study subjects participated. The proportion of chronic patients’ intention to use PHR was 46.7% (95.0% CI (43.4-50.1)). According to the findings, performance expectancy (β=0.259, p-value<0.001), effort expectancy (β=0.214, p-value<0.001), social influence (β=0.174, p-value<0.001), and facilitating condition (β=0.114, p-value<0.01) had a significant effect on the intention to use PHRs.
Conclusion: Generally, the overall intention to use PHR was low. Our finding illustrates that the effects of performance expectancy, effort expectancy, social influence, and facilitating conditions had a positive effect on patients’ intentions to use PHRs. The effect of effort expectancy on the intention to use a PHR was positively moderated by age. In addition, implementers should focus on improving patient capacity, motivating users, and raising awareness regarding PHR.
Assessment of Current Practices and Emerging Trends: A Pilot Survey of the Thyroid Disease Committee of the Sichuan Medical Association
Authors: Zhang Sicheng , Zeng Dingfen , Shui Chunyan , Li Chao 
Published: August 2023

Abstract
Background
The Thyroid Disease Committee of the Sichuan Medical Association (TDCSMA) conducted a periodic survey to assess the current state of thyroid-related treatment practices in Sichuan Province.
Methods
A comprehensive 40-question survey was distributed to 44 thyroid-related departments across the hospitals in Sichuan province. Data collection took place from June 15th to June 30th.
Results
The hospitals surveyed are mainly situated in the eastern plains of Sichuan. Of these hospitals, 52.27% have 31–60 beds, and 54.55% of the departments are staffed by 5–10 doctors. Most doctors were born between 1990 and 2000. In terms of specialization, 75% of the surveyed departments have doctors majored in thyroid and breast, 47.73% in general surgery, and 6.82% each in otolaryngology and vascular surgery. The survey respondents have diverse years of training before entering practice: 27.27% have 2–3 years, 31.82% have 3–4 years, and 20.45% have 4–5 years. Thyroid cancer accounts for 100% of the responses, followed by thyroid adenoma (90.91%) and thyroid cyst (81.82%). Regarding thyroid surgery, 54.54% of the departments perform 100–500 open thyroid surgeries annually, and 72.73% conduct more than 10 endoscopic-assisted thyroid surgeries each year. With the implementation of China's medical insurance reform in 2023, 52.27% of the hospitals adjust prices for thyroid surgery. Additionally, 52.27% of the departments report that their doctors work over 50 hours per week, and the overall career satisfaction rate is 75% (very satisfied or satisfied). Moreover, 34.09% of department heads report a decrease in average patient intake from 2020 to 2023, while the operative volume increased for 72.73% of the surveyed departments in the past 12 months.
Conclusions
The data provides a comprehensive and current overview of the thyroid-related departments, covering medical status, disease distribution, case and operative volume, and career satisfaction. The findings indicate a relative imbalance in the allocation of medical resources for thyroid diseases within Sichuan Province.
Self-perceptions’ knowledge, training, management and personal experience in psychiatric emergencies according to work experience: an explorative study among Italian nurses employed in the Emergency Departments
Authors: Chang Yun-Chen , Mea Rocco , Vitale Elsa 
Published: August 2023

Abstract

Background: In Italy, the number of incoming arrives in Emergency Departments (ED) that include psychiatric patients has significantly increased. To assess how ED nurses perceived their self-perceptions’ knowledge, the arguments of the greatest interest in the training and management of psychiatric emergencies and also personal experience in emergency psychiatric patients according to both nursing educational levels and work experience.
Methods: An observational, cohort, cross-sectional and multicenter study was conducted from August to September 2022.
Results: This study was completed with 413 Italian nurses surveyed. Knowledge in psychiatric emergency conditions was important for an emergency nurse (t=2.444, p=.015), and nurses considered as important current knowledge on issues of relevant to emergency psychiatry (t=3.516, p<.001). Significant associations were assessed between: nursing educational level and psychiatric aggressions received (t=-2.675, p=0.008) and arguments of interactions with psychiatric drugs (t=-2.106; p=0.036); with the hypochondriacally anxiety (t=-2.274; p=0.024); the hallucinated patients (t=2858; p=0.004); the use of counseling in psychiatric wards (t=1.555, p=0.121). Significant associations were recorded between work experience and pharmacological management of the psychiatric patients (t=-3.035; p=.003); psychiatric drugs in emergency (t=2.758; p=.006); medico-legal aspects in psychiatric emergencies (t=2.554; p=.011); the management of negative experiences of the multidisciplinary team towards the patient (t=-2.684; p=.008); patients who simulated (t=-2.138; p=.033).
Conclusions: ED nurses required to perform their knowledge to identify the psychological and the physical signs and symptoms of psychological distress as well as the skills to provide appropriate care at the EDs, by improving nurses’ competencies to recognize and manage psychiatric patients.
Explaining the resources wastage and its underlying factors in COVID-19 management in Iran: A qualitative study
Authors: vatandoost vahid , barooni mohsen , Tabatabaee Seyed Saeed , Okhovati maryam 
Published: August 2023

Abstract
Background:
Identifying factors affecting health costs can be useful in determining the best policies for controlling and managing health costs. To this end, the present study aimed to analyze resource wastage and identify factors underlying it in COVID-19 management in the Iranian health sector.
Method:
This qualitative content analysis study was conducted in the Iranian health sector. The participants were 23 senior, middle, and operational managers in the health sector. The data were collected through semi-structured interviews with the managers. The participants were selected using purposive, quota, and snowball sampling techniques. The interviews continued until the data were saturated. The collected data were analyzed using MAXQDA software (version 10).
Results:
Following the data analysis, the factors affecting the wastage of health resources were divided into 4 categories and 9 subcategories. Vaccines, diagnostic kits, medicines, and human resources were the main factors underlying resource wastage. The identified subcategories were open and unused vials, nonuse of distributed vaccines and their expiration, excess supply and decreased demand for vaccines, expiration of diagnostic and laboratory kits and their quantitative and qualitative defects, the large number of tests, non-organization of medical staff, and the inefficiency and expiration of medicines.
Conclusion:
Given the limited health funds and the increased healthcare costs, effective preparation and planning and making reasonable decisions for unexpected events can minimize unnecessary costs and resource wastage, which requires some revisions in attitudes toward COVID-19 management in the healthcare sector.
WITHDRAWN: An Extension of the Geometric G Family of Probability Distributions with Statistical Properties, Copulas and Modeling Real-life Data Sets
Authors: Hashem Atef F. , Abdelkawy M. A. , Muse Abdisalam Hassan , Yousof Haitham M. 
Published: August 2023

Abstract
A novel compounded two-parameters-G family of continuous probability distributions is presented here thanks to this body of work. Calculations are done to determine and examine the pertinent mathematical properties. There is a dedicated focus on the typical reciprocal-Weibull baseline model both mathematically and statistically in one of the special parts. We used the copula method to generate a number of bivariate and multivariate distributions. These new distributions will be useful for modeling bivariate and multivariate data. The new compounded two-parameters-G family's applicability and flexibility are demonstrated by means of three applications to real-world data sets. These examples prove the family's applicability.
Genome-wide investigation of the TGF-β superfamily in scallops
Authors: Zhang Qian , Chen Jianming , Wang Wei 
Published: August 2023

Abstract
Background
Transforming growth factor β (TGF-β) superfamily genes can regulate various processes, especially in embryogenesis and adult development and homeostasis. To understand the evolution and divergence patterns of the TGF-β superfamily in scallops, genome-wide data from the Bay scallop (Argopecten irradians), the Zhikong scallop (Chlamys farreri) and the Yesso scallop (Mizuhopecten yessoensis) were systematically analyzed using bioinformatics methods.
Results
Twelve members of the TGF-β superfamily were identified for each scallop. The phylogenetic tree supported that these genes were grouped into 11 clusters, including BMPs, ADMP, NODAL, GDF, activin/inhibin and AMH. The number of exons and the conserved motif showed some differences between different clusters, while genes in the same cluster showed a high degree of similarity. Selective pressure analysis showed that the TGF-β superfamily in scallops was evolutionarily conserved. The spatiotemporal expression profiles suggested that different TGF-β members have distinct functions. Several BMP-like and NODAL-like genes were highly expressed in early developmental stages, patterning the embryonic body plan. GDF8/11-like genes showed high expression in striated muscle and smooth muscle, suggesting that these genes may play a critical role in regulating muscle growth. Further analysis revealed a possible duplication of AMH, which played a key role in gonadal growth/maturation in scallops. In addition, this study found that several genes were involved in heat and hypoxia stress in scallops, providing new insight into the function of the TGF-β superfamily.
Conclusion
Characteristics of the TGF-β superfamily in scallops were identified, including sequence structure, phylogenetic relationships, and selection pressure. The expression profiles of these genes in different tissues, at different developmental stages and under different stresses were investigated. Generally, the current study lays a foundation for further study of their pleiotropic biological functions in scallops.
Interacting proteins of AMPK studied using TurboID proximity labeling technology
Authors: Guo Jieyu , Lu Siao , Zhao Baoqing , Gong Jun , Wang Long , Ding Liqiong , Chen Qingjie , Liu Wu 
Published: August 2023

Abstract
Objective
Adenosine monophosphate activated protein kinase (AMPK), whose activity is regulated by the AMP/ATP ratio in the body, is an important center for controlling cell energy metabolism, and is also an evolutionarily conservative serine/threonine protein kinase. AMPK plays an important role in diabetes, myocardial infarction and many diseases. We try to use TurboID technology to study a novel protein that can interact with AMPK, and explore the biological function of this protein, in order to provide a theoretical basis for the development of new targeted drugs.
Methods
We will construct AMPK overexpression stable cell lines by transfecting AMPK-TurboID fusion gene into astrocytes U251 using lentiviral infection technique. After 6 h of biotin labeling, a large number of proteins interacting with AMPK can be observed by silver staining. The interacting proteins were analyzed by label-free quantitative protein profiling, and the interacting protein DNAJA1 was selected for IP and immunofluorescence validation.
Results
We successfully constructed AMPK-TurboID overexpression stable cell lines, and obtained a large number of interacting proteins after biotin labeling experiments, and then obtained all interacting protein information by mass spectrometry, and selected the interacting protein DNAJA1 for IP and immunofluorescence validation. In addition, we found that AMPK and DNAJA1 could be jointly involved in anti-apoptotic cell death.
Conclusion
Because AMPK is involved in a variety of metabolic pathways, coupled with the advantages of high catalytic activity and fast labeling of TurboID neighboring labeling technology, a large number of proteins interacting with AMPK gene were found through biotin labeling experiments, and subsequent experiments verified that AMPK and DNAJA1 have interaction, and the two can synergistically protect cells from apoptosis; this has laid a certain theoretical foundation for how to use AMPK to treat clinical diseases such as diabetes and myocardial infarction in the future.
Public acceptance of default nudges to promote healthy and sustainable food choices
Authors: Lemken Dominic , Wahnschafft Simone , Eggers Carolin 
Published: August 2023

Abstract
Background: Default nudges are an increasingly prominent tool for promoting healthy and sustainable food choices; however, questions of acceptance remain. While default nudges are more acceptable to the public than traditionally paternalistic tools that aim to restrict choice, they are also the least acceptable amongst nudging strategies. Little research has investigated the aspects of default nudge design that can be leveraged to better uphold freedom of choice, increase public acceptance, and therefore heighten legitimacy of default nudges. Consequently, this study examines public acceptance of five food choice default nudges with demonstrated precedent of effectiveness, as drawn from research studies and/or real-world policies, along with a design variation of each anticipated to increase acceptance. Three drivers of acceptance – perceived intrusiveness, perceived effectiveness, and own behavior – are examined.
Methods: An online survey was administered in Germany (N = 451) to a sample representative of the adult population on quotas of age, gender and income. Acceptance and drivers were measured using seven-point Likert scales. Significant differences in median acceptance of the nudge were determined and displayed graphically. Ten proportional odds ordered logit models were applied and estimated using a maximum likelihood approach to investigate the mechanisms of nudge acceptance.
Results: Examined changes in nudge design, particularly decreasing costliness of opting out and increasing transparency, increased the acceptance of three of the five nudges (N2.2: p = 0.000; N3.2: p = 0.000; N4.2: p = 0.008). Perceived intrusiveness emerged as the most prominent driver of acceptance (negative relationship), followed by perceived effectiveness (positive relationship). Own engagement in the target behavior of the nudge and socio-demographic variables demonstrated negligible impact on acceptance.
Conclusions: Mitigating the costliness of opting out and improving nudge transparency emerge as key opportunities for choice architects to improve public acceptance, and thereby potentially identify 'sweet spots' in designing default nudges that are both effective and acceptable. The protection of individual freedom of choice and effectiveness are key aspects for choice architects to communicate to increase acceptance.
The screening of miRNA/transcription factors-target gene regulatory networks in the aging process of hematopoietic stem cells
Authors: Gong Xue , Wei Shuyao , Dou Lu , Mou Fangzhen 
Published: August 2023

Abstract
Background
hematopoietic stem cells (HSCs) failure is one of the key signs of biological aging.
Objective
Screening the key gene regulatory network in the aging process.
Materials and methods
Used GES32719 and GSE69408 to screen the difference genes in the Aging process, and enrichment analysis and protein-protein interaction analysis were carried out to obtain key transcription factor (TFs). The transcription factor is matched to the DNA damage map to obtain a target gene. Analysis of miRNA regulated or was regulated with transcription factors and target genes. Based on the above results, construct possible miRNA/TFs/target gene regulation networks.
Results
222 different genes were obtained using the GES32719, found mitogen-activated protein kinase signaling pathway, microRNAs in cancer, and the hippo signaling pathway was significantly enrichment. Four TFS (MYC, FOS, ERG1, and JUN) were identified. Docked with DNA damage map, obtained three key target genes (PTEN, PFN1, and LMNA). 13 potential miRNAs based on TFS and target gene, where has-miR-139-5p and has-miR-543 were considered the most critical. Based on these data, three possible miRNA/TFs/target gene regulatory networks were constructed.
Conclusion
We constructed three possible regulatory models. This study provides a reference and basis for studying the possible mechanisms of HSCs aging.
Influence of size on the compressive properties of cellular structures manufactured by additive technologies (FFF, SLS).
Authors: Rudnik Mateusz Paweł 
Published: August 2023

Abstract
The aim of this paper was to compare manufactured cell structures with an appropriately defined formula. Printed models of the hexagonal structure subjected to compression showed that, in the case of the Polylactic Acid Blue material, as the size of the side length of the hexagonal cells increased, the quality of the generated diagrams also increased, which informed the undesired effects of the compressive force in the tests. In the case of cells manufactured from the PA2200 material, it was noted that the maximum force acting on the cell structure decreased with increasing cell side length, however, no undesirable situations occurred during testing in contrast to structures made from Polylactic Acid base materials. In the case of Polylactic Acidmaterials, special attention had to be paid to the Polylactic Acid Gray material. The models were printed with the same parameters, from the same Stereolitography language file, had a slightly higher mass and were subjected to the same compression test, yet showed significant differences in the tests carried out compared to the other models.