bluuebunny/crossref_metadata_2025_split huggingface dataset:
Cucumber NUCLEAR FACTOR-YC2/-YC9 target translocon component CsTIC21 in chloroplast photomorphogenesis
Authors: Ke Xubo , Shen Junjun , Niu Yuqian , Zhao Hongjiao , Guo Yalu , Sun Piaoyun , Yang Tongwen , Jiang Yanxin , Zhao Bosi , Wang Zheng , Wu Tao , Wang Huasen , Li Zheng 
Published: May 2023

Abstract
Light signals promote photomorphogenesis and photosynthesis, allowing plants to establish photoautotrophic growth. Chloroplasts are organelles responsible for photosynthesis in which light energy is converted into chemical energy and stored as organic matter. However, how light regulates chloroplast photomorphogenesis remains unclear. Here, we isolated a cucumber (Cucumis sativus L.) mutant albino seedling (as) from an ethyl methane sulfonate mutagenesis library with an albino phenotype. Map-based cloning revealed that the mutation occurred in a component of cucumber translocon at the inner membrane of chloroplasts (CsTIC21). Subsequently, virus-induced gene silencing and CRISPR/Cas9 analyses confirmed the association between the mutant gene and the as phenotype. Loss-of-function of CsTIC21 induces malformation of chloroplast formation, leading to albinism and death in cucumber. Notably, CsTIC21 transcription was very low in etiolated seedlings grown in the dark and was upregulated by light, with expression patterns similar to those of Nuclear factor-YC (NF-YC) genes. Here, 7 cucumber NF-YC family genes (CsNF-YC) were identified, among which the expression of 4 genes (CsNF-YC1, -YC2, -YC9, and -YC13) responded to light. Gene silencing of all CsNF-YC genes in cucumber indicated that CsNF-YC2, -YC9, -YC11-1, and -YC11-2 induced distinct etiolated growth and decreased chlorophyll content. Interaction studies verified that CsNF-YC2 and CsNF-YC9 target the CsTIC21 promoter directly and promote gene transcription. These findings provide mechanistic insights on the role of the NF-YCs–TIC21 module in chloroplast photomorphogenesis promoted by light in cucumber.
Inflation and Individual Investors’ Behavior: Evidence from the German Hyperinflation
Authors: Braggion Fabio , von Meyerinck Felix , Schaub Nic 
Published: May 2023

Abstract
We analyze how individual investors respond to inflation. We introduce a unique data set containing information on local inflation and security portfolios of more than 2,000 clients of a German bank between 1920 and 1924, covering the German hyperinflation. We find that individual investors buy fewer (sell more) stocks when facing higher local inflation. This effect is more pronounced for less sophisticated investors. Moreover, we document a positive relation between local inflation and forgone returns following stock sales. Our findings are consistent with individual investors suffering from money illusion. Alternative explanations, such as consumption needs, are unlikely to drive our results.
Authors have furnished an Internet Appendix, which is available on the Oxford University Press Web site next to the link to the final published paper online
The effect of antimalarials on the safety and persistence of treatment with biologic agents or Janus kinase inhibitors in rheumatoid arthritis
Authors: Bredemeier Markus , Duarte Ângela L , Pinheiro Marcelo M , Kahlow Bárbara S , Macieira José C , Ranza Roberto , Miranda José R , Valim Valéria , de Castro Gláucio R , Bértolo Manoel B , Sauma Maria F , Fernandes Vander , Ribeiro Ana C , Teodoro Reginaldo B , Brenol Claiton V , Carvalho Hellen M , Studart Sâmia A , Pinheiro Geraldo R , da Rocha Laurindo F , de Lima Hugo D , Pereira Ivânio A , Gazzeta Morgana O , Kakehasi Adriana M , Louzada Paulo , Hayata André L , Lupo Cristiano M , da Silveira Inês G , Kowalski Sergio C , Titton David C , Chakr Rafael M , Ranzolin Aline , Xavier Ricardo M , Laurindo Ieda M 
Published: May 2023

Abstract

Objectives
To test the association of use of antimalarials with the overall safety of treatment in RA patients receiving one or multiple courses of biologic (b)DMARDs or a Janus kinase inhibitor (JAKi).

Methods
BiobadaBrasil is a multicentric registry-based cohort study of Brazilian patients with rheumatic diseases starting their first bDMARD or JAKi. The present analysis includes RA patients recruited from January 2009 to October 2019, followed up over one or multiple (up to six) courses of treatment (latest date, 19 November 2019). The primary outcome was the incidence of serious adverse events (SAEs). Total and system-specific adverse events (AEs) and treatment interruption served as secondary outcomes. Negative binomial regression with generalized estimating equations (to estimate multivariate incidence rate ratios, mIRR) and frailty Cox proportional hazards models were used for statistical analyses.

Results
The number of patients enrolled was 1316 (2335 treatment courses, 6711 patient-years [PY]; 1254.5 PY on antimalarials). The overall incidence of SAEs was 9.2/100 PY. Antimalarials were associated with reduced risk of SAEs (mIRR: 0.49; 95% CI: 0.36, 0.68; P &lt; 0.001), total AEs (0.68; 95% CI: 0.56, 0.81; P &lt; 0.001), serious infections (0.53; 95% CI: 0.34, 0.84; P = 0.007) and total hepatic AEs (0.21; 95% CI: 0.05, 0.85; P = 0.028). Antimalarials were also related to better survival of treatment course (P = 0.003). There was no significant increase in the risk of cardiovascular AEs.

Conclusion
Among RA patients on treatment with bDMARDs or JAKi, concomitant use of antimalarials was associated with reduced the incidence of serious and total AEs and with longer treatment course survival.
Steady decrease in systemic sclerosis mortality rates at younger ages over the past five decades
Authors: Yen Eric Y , Singh Devanshu R , Singh Ram Raj 
Published: May 2023

Abstract

Objective
We aimed to assess SSc mortality by age in the general population over the past five decades.

Methods
This is a population-based study using a national mortality database and the census data for all US residents. We calculated the proportions of deaths for SSc and for all other causes (non-SSc) by age, and calculated age-standardized mortality rates (ASMRs) for SSc and non-SSc, and the ratio of SSc-ASMR to non-SSc-ASMR by age groups for each year from 1968 through 2015. We performed joinpoint regression to estimate the average annual percent change (AAPC) for each of these parameters.

Results
SSc was recorded as the underlying cause of death in 5457 decedents aged ≤44 years, 18 395 aged 45–64, and 22 946 aged ≥65 from 1968 through 2015. At ages ≤44, the proportion of annual deaths decreased more for SSc than for non-SSc: AAPC, –2.2% (95% CI, –2.4% to –2.0%) for SSc vs –1.5% (–1.9% to –1.1%) for non-SSc. Consistently, SSc-ASMR decreased from 1.0 (95% CI, 0.8–1.2) in 1968 to 0.4 (0.3–0.5) per million persons in 2015, a cumulative decrease of 60% at an AAPC of –1.9% (95% CI, –2.5% to –1.2%) at ages ≤44. The SSc-ASMR:non-SSc-ASMR ratio also decreased [cumulative –20%; AAPC –0.3% (95% CI, –1.15% to 0.55%)] in the ≤44-years group. In contrast, those aged ≥65 experienced a steep increase in the SSc-ASMR [cumulative 187.0%; AAPC 2.0% (95% CI, 1.8–2.2)] and the SSc-ASMR:non-SSc-ASMR ratio [cumulative 395.4%; AAPC 3.3% (95% CI, 2.9–3.7)].

Conclusion
Mortality for SSc has steadily decreased at younger ages over the past five decades.
Variation within major internal repeats of KSHV in vivo
Authors: Santiago Jan Clement , Westfall Dylan H , Adams Scott V , Okuku Fred , Phipps Warren , Mullins James I 
Published: May 2023

Abstract
Kaposi’s sarcoma-associated herpesvirus (KSHV) is the etiologic agent of Kaposi’s sarcoma (KS), yet the viral genetic factors that lead to the development of KS in KSHV-infected individuals have not been fully elucidated. Nearly, all previous analyses of KSHV genomic evolution and diversity have excluded the three major internal repeat regions: the two origins of lytic replication, internal repeats 1 and 2 (IR1 and IR2), and the latency-associated nuclear antigen (LANA) repeat domain (LANAr). These regions encode protein domains that are essential to the KSHV infection cycle but have been rarely sequenced due to their extended repetitive nature and high guanine and cytosine (GC) content. The limited data available suggest that their sequences and repeat lengths are more heterogeneous across individuals than in the remainder of the KSHV genome. To assess their diversity, the full-length IR1, IR2, and LANAr sequences, tagged with unique molecular identifiers (UMIs), were obtained by Pacific Biosciences’ single-molecule real-time sequencing (SMRT-UMI) from twenty-four tumors and six matching oral swabs from sixteen adults in Uganda with advanced KS. Intra-host single-nucleotide variation involved an average of 0.16 per cent of base positions in the repeat regions compared to a nearly identical average of 0.17 per cent of base positions in the remainder of the genome. Tandem repeat unit (TRU) counts varied by only one from the intra-host consensus in a majority of individuals. Including the TRU indels, the average intra-host pairwise identity was 98.3 per cent for IR1, 99.6 per cent for IR2 and 98.9 per cent for LANAr. More individuals had mismatches and variable TRU counts in IR1 (twelve/sixteen) than in IR2 (two/sixteen). There were no open reading frames in the Kaposin coding sequence inside IR2 in at least fifty-five of ninety-six sequences. In summary, the KSHV major internal repeats, like the rest of the genome in individuals with KS, have low diversity. IR1 was the most variable among the repeats, and no intact Kaposin reading frames were present in IR2 of the majority of genomes sampled.
Heart failure
Authors: Vuckovic Karen M. , Ryan Catherine , Gomez Yorman , Bierle Rebecca 
Published: May 2023

Abstract:
Heart failure (HF) is a chronic syndrome that requires patients to manage signs and symptoms and adhere to a complex medication regimen. This article discusses updates in HF care related to a universal definition and new therapies, focusing on the four pillars of therapy for HF with reduced ejection fraction.
Autoregulatory Cerebral Perfusion Pressure Insults in Traumatic Brain Injury and Aneurysmal Subarachnoid Hemorrhage: The Role of Insult Intensity and Duration on Clinical Outcome
Authors: Svedung Wettervik Teodor , Hånell Anders , Howells Timothy , Engström Elisabeth R. , Lewén Anders , Enblad Per 
Published: May 2023

Background:
This single-center, retrospective study investigated the outcome effect of the combined intensity and duration of differences between actual cerebral perfusion pressure (CPP) and optimal cerebral perfusion pressure (CPPopt), and also for absolute CPP, in patients with traumatic brain injury (TBI) and aneurysmal subarachnoid hemorrhage (aSAH).

Methods:
A total of 378 TBI and 432 aSAH patients treated in a neurointensive care unit between 2008 and 2018 with at least 24 hours of CPPopt data during the first 10 days following injury, and with 6-month (TBI) or 12-month (aSAH) extended Glasgow Outcome Scale (GOS-E) scores, were included in the study. ∆CPPopt-insults (∆CPPopt=actual CPP−CPPopt) and CPP-insults were visualized as 2-dimensional plots to highlight the combined effect of insult intensity (mm Hg) and duration (min) on patient outcome.

Results:
In TBI patients, a zone of ∆CPPopt ± 10 mm Hg was associated with more favorable outcome, with transitions towards unfavorable outcome above and below this zone. CPP in the range of 60 to 80 mm Hg was associated with higher GOS-E, whereas CPP outside this range was associated with lower GOS-E. In aSAH patients, there was no clear transition from higher to lower GOS-E for ∆CPPopt-insults; however, there was a transition from favorable to unfavorable outcome when CPP was <80 mm Hg.

Conclusions:
TBI patients with CPP close to CPPopt exhibited better clinical outcomes, and absolute CPP within the 60 to 80 mm Hg range was also associated with favorable outcome. In aSAH patients, there was no clear transition for ∆CPPopt-insults in relation to outcome, whereas generally high absolute CPP values were associated overall with favorable recovery.
Ambient PM2.5 and acute incidence of myocardial infarction in China: a case-crossover study and health impact assessment
Authors: Ban Jie , Ma Runmei , Liu An , Wang Qing , Chen Chen , Sun Qinghua , Wang Yanwen , Hu Jianlin , Li Tiantian 
Published: May 2023

Background and purpose:
Evidence investigating the association between ambient fine particulate matters (PM2.5) and acute incidence of myocardial infarction in developing countries is limited. This study aims to investigate linear and nonlinear patterns for the association between PM2.5 and acute incidence of myocardial infarction based on multicounty registry dataset and evaluate the reduction of premature myocardial infarction incidence under different pollution control objectives in China.

Methods:
Thirty-six thousand six hundred and seventy-nine registered myocardial infarction incidence cases from 15 Chinese counties from January 1, 2013, to December 31, 2017, were obtained. We adopted a time-stratified case-crossover design with conditional logistic regression models.

Results:
With a 10 μg/m3 increase in PM2.5 exposure, there was an increase of 0.98% (95% CI: 0.40%–1.57%) in acute incidence risk of myocardial infarction. The corresponding values for males and individuals aged over 74 years were 1.58% (95% CI: 0.82%–2.35%) and 1.19% (95% CI: 0.35%–2.05%) respectively, indicating higher risks than other groups. The nonlinear concentration–response curve indicated a steeper slope under daily exposure below 50 μg/m3 and the marginal avoided premature morbidity became larger under the current air quality standard.

Conclusions:
The robust findings from this study may suggest the necessity for a continuous reduction of PM2.5 exposure concentration from the perspectives of public health.
Fluorescence In Situ Hybridization as Diagnostic Tool for Implant-associated Infections: A Pilot Study on Added Value
Authors: Scheuermann-Poley Catharina , Wiessner Alexandra , Kikhney Judith , Gatzer Renate , Müller Martin , Stichling Marcus , Moter Annette , Willy Christian 
Published: May 2023

Background:
Implant-associated infections are a devastating complication in surgery. Especially in infections with biofilm-forming microorganisms, the identification of the causing microorganism remains a challenge. However, the classification as biofilm is not possible with conventional polymerase chain reaction or culture-based diagnostics. The aim of this study was to evaluate the additional value of fluorescence in situ hybridization (FISH) and nucleic acid amplification technique (FISHseq) to discuss a diagnostic benefit of the culture-independent methods and to map spatial organization of pathogens and microbial biofilms in wounds.

Methods:
In total, 118 tissue samples from 60 patients with clinically suspected implant-associated infections (n = 32 joint replacements, n = 24 open reduction and internal fixation, n = 4 projectiles) were analyzed using classic microbiological culture and culture-independent FISH in combination with polymerase chain reaction and sequencing (FISHseq).

Results:
In 56 of 60 wounds, FISHseq achieved an added value. FISHseq confirmed the result of cultural microbiological examinations in 41 of the 60 wounds. In 12 wounds, one or more additional pathogens were detected by FISHseq. FISHseq could show that the bacteria initially detected by culture corresponded to a contamination in three wounds and could exclude that the identified commensal pathogens were a contamination in four other wounds. In five wounds, a nonplanktonic bacterial life form was detected.

Conclusions:
The study revealed that FISHseq gives additional diagnostic information, including therapy-relevant findings that were missed by culture. In addition, nonplanktonic bacterial life forms could also be detected with FISHseq, albeit less frequently than previously indicated.
Lateral Canthotomy Revisited: A Refined Surgical Approach for Orbital Access
Authors: Soliman Luke , Rhee Ben , Lerner Julia L. , Sobti Nikhil , Rao Vinay , Woo Albert S. 
Published: May 2023

Summary:
The transconjunctival incision is a common and effective approach for establishing surgical exposure to the orbital floor. When access to the lateral orbit is also required, this incision may be extended by an accompanying lateral canthotomy, which releases the tarsal plates from the conjunctiva. Although this approach broadens surgical access through a simple extension, it is often remarked for unpredictable healing patterns and negative aesthetic sequelae, such as rounding of the lateral canthal angle. Traditionally, lateral canthotomy is performed by a transverse incision through a natural skin crease of the lateral palpebral fissure. Herein, we discuss our experience with a less common approach to lateral canthotomy, in which only the inferior crus of the lateral canthal tendon is divided. This approach limits manipulation of delicate orbital anatomy and aims to minimize unsightly scarring while still affording excellent visualization of the lateral orbit and orbital floor.
The Impact of Prior Breast Augmentation on Breast Reconstruction after Mastectomy
Authors: Clegg Devin J. , Salomon Brett J. , Porter Christopher G. , Mazonas Thomas W. , Heidel Robert E. , Stephenson Stacy M. , Herbig Kathleen S. , Chun Joseph T. , Lloyd Jillian M. , Boukovalas Stefanos 
Published: May 2023

Background:
The risk of women developing breast cancer after augmentation mammaplasty may be lower than the general population, with minimal current literature on breast reconstruction in this population. We sought to evaluate the impact of previous augmentation on postmastectomy breast reconstruction.

Methods:
Retrospective review of patients who underwent mastectomies from 2017 to 2021 at our institution was performed. Analysis included frequencies and percentages, descriptive statistics, chi-square analysis, and Fisher exact test.

Results:
Four hundred seventy patients were included, with average body mass index of 29.1 kg/m2, 96% identifying as White, and an average age at diagnosis of 59.3 years. Twenty (4.2%) patients had a prior breast augmentation. Reconstruction was performed in 80% of the previously augmented patients compared to 49.9% of nonaugmented patients (P = 0.01). Reconstruction was alloplastic in 100% of augmented and 88.7% of nonaugmented patients (P = 0.15). All reconstructed augmented patients underwent immediate reconstruction compared with 90.5% of nonaugmented patients (P = 0.37), and two-stage reconstruction was most common (75.0% versus 63.5%; P = 0.42). Of the previously augmented patients, 87.5% increased implant volume, 75% underwent same implant plane reconstruction, and 68.75% underwent same implant-type reconstruction as their augmentation.

Conclusions:
Previously augmented patients were more likely to undergo reconstruction after mastectomy at our institution. All reconstructed augmented patients underwent alloplastic reconstruction, with most performed immediately in staged fashion. Most patients favored silicone implants and maintained the same implant type and plane of reconstruction, with increased implant volume. Larger studies are required to further investigate these trends.
Does Symptom-Onset Treatment With Sertraline Improve Functional Impairment for Individuals With Premenstrual Dysphoric Disorder?
Authors: Yonkers Kimberly A. , Altemus Margaret , Gilstad-Hayden Kathryn , Kornstein Susan G. , Gueorguieva Ralitza 
Published: May 2023

Abstract

Purpose/Background
Daily treatment with sertraline improves functional impairment among individuals with premenstrual dysphoric disorder (PMDD). We do not know whether treatment initiated at symptom onset also improves functional impairment.

Methods/Procedures
This 3-site, double blind, randomized, clinical trial compared sertraline (25–100 mg) to similar appearing placebo, both administered at symptom onset, for reduction of PMDD symptoms. Ninety participants were allocated to sertraline and 94 participants to placebo. Functional outcomes from the Daily Ratings of the Severity of Problems included (1) reduced productivity or efficiency at work, school, home, or daily routine; (2) interference with hobbies or social activities; and (3) interference with relationships. Items were measured from 1 (no interference) to 6 (extreme interference) and averaged for the final 5 luteal phase days. This secondary analysis examined whether improvement in functional domains was greater for those allocated to sertraline compared with placebo. Second, we used causal mediation analyses to explore whether specific PMDD symptoms mediated functional improvement.

Results/Findings
Only relationship functioning improved significantly with active treatment between baseline and the end of the second cycle (active group mean [SD] change, −1.39 [1.38]; placebo group mean change, −0.76 [1.20]; β = −0.40; SE, 0.15; P = 0.009). The total effect of treatment on interference was −0.37 (95% confidence interval [CI], −0.66 to −0.09; P = 0.011). Given the nonsignificant direct effect (0.11; 95% CI, −0.07 to 0.29; P = 0.24) and significant indirect effect (−0.48; 95% CI, −0.71 to −0.24; P < 0.001), amelioration of anger/irritability likely mediated reductions in relationship interference.

Implications/Conclusions
That anger/irritability mediates impairments in relationship functioning has face validity but should be replicated in other data sets.

Trial Registration

ClinicalTrials.gov identifier NCT00536198.
Drain fluid biomarkers for the diagnosis of clinically relevant postoperative pancreatic fistula - a diagnostic accuracy systematic review and meta-analysis
Authors: Rykina-Tameeva Nadya , MacCulloch Daniel , Hipperson Luke , Ulyannikova Yulia , Samra Jaswinder S , Mittal Anubhav , Sahni Sumit 
Published: May 2023

Background:
Pancreatectomy is the only curative treatment available for pancreatic cancer and a necessity for patients with challenging pancreatic pathology. To optimise outcomes, postsurgical complications such as clinically relevant postoperative pancreatic fistula (CR-POPF) should be minimised. Central to this is the ability to predict and diagnose CR-POPF, potentially through drain fluid biomarkers. This study aimed to assess the utility of drain fluid biomarkers for predicting CR-POPF by conducting a diagnostic test accuracy systematic review and meta-analysis.

Methods:
Five databases were searched for relevant and original papers published from January 2000 – December 2021, with citation chaining capturing additional studies. The QUADAS-2 tool was used to assess the risk of bias and concerns regarding applicability of the selected studies.

Results:
Seventy-eight papers were included in the meta-analysis, encompassing six drain biomarkers and 30758 patients with a CR-POPF prevalence of 17.42%. The pooled sensitivity and specificity for 15 cut-offs was determined. Potential triage tests (Negative Predictive Value>90%) were identified for the ruling-out of CR-POPF and included post-operative day 1 (POD1) drain amylase in pancreatoduodenectomy (PD) patients (300 U/L) and in mixed surgical cohorts (2500 U/L), POD3 drain amylase in PD patients (1000-1010 U/L) and drain lipase in mixed surgery groups (180 U/L). Notably, drain POD3 lipase had a higher sensitivity than POD3 amylase, while POD3 amylase had a higher specificity than POD1.

Conclusions:
The current findings using the pooled cut-offs will offer options for clinicians seeking to identify patients for quicker recovery. Improving the reporting of future diagnostic test studies will further clarify the diagnostic utility of drain fluid biomarkers, facilitating their inclusion in multi-variable risk-stratification models and the improvement of pancreatectomy outcomes.