bluuebunny/crossref_metadata_2025_split huggingface dataset:
Spontaneous intramuscular hemorrhage in cancer-associated dermatomyositis: a case and literature review
Authors: Xing Rui , Shen Guifen , Xiang Fenfen , Dong Lingli 
Published: March 2023

Abstract
Background: Dermatomyositis (DM) is a systemic autoimmune disease with characteristic cutaneous manifestations and muscle weakness of the trunk, symmetrical proximal muscles of the limbs. Spontaneous intramuscular hemorrhage (SIH) is a rare but life-threatening complication of DM. The pathogenetic mechanism and management of intramuscular hematoma in these patients remains unclear. Here we discuss a case of recurrent hemorrhage in a patient with DM, and reviews the relevant literature in order to make timely diagnosis and treatment.
Case presentation:The patient is a 53-year-old male who presented with rashes, muscle weakness and dysphagia and was diagnosed with DM. During treatment, he developed SIH of the arm and right psoas major muscle, MRI showed extensive edema of the right shoulder girdle muscle and muscle groups of the upper arm, CT scan showed new-onset hematoma formation in the right psoas major muscle. The detection of D-dimer, TAT, PIC and t-PAIC indicated that he has thrombosis and hyperfibrinolysis. Blood transfusion and supportive treatment was immediately performed, the hematoma did not expand. Abdominal distension has not been relieved after active treatment. Electronic gastroscopy found gastric sinus ulcer, histopathology of biopsy showed signet-ring cell carcinoma.
Conclusions: Patients with cancer-associated DM have an increased risk of thrombosis, conventional anticoagulation therapy is not recommended. If the patient has a high risk of thrombosis and needs drug intervention, it is important to dynamic monitor the coagulation parameters during anticoagulation therapy. Especially when the D-dimer is high, it is uncertain whether the patient is in the state of thrombosis or hyperfibrinolysis, the detection of TAT, PIC, t-PAIC can help to determine whether to initiate anticoagulation therapy.
Strength prediction model of fractured dolomite and analysis of mechanical properties based on PFC3D
Authors: Chen Yi , Rao Junying , Zhao Changjie , Xue Yanghao , Liu Chang , Yin Quan 
Published: March 2023

Abstract
In order to study the mechanical properties of fractured dolomite, this paper first uses the rubbing method to count the surface fracture of the dolomite rock samples, obtains the main parameters of the fracture characteristics, and then carries out the uniaxial compression test to obtain the uniaxial compressive strength of the dolomite rock. Then, the Pearson correlation coefficient and the maximum information coefficient (MIC) are used to analyze the fracture characteristics. The three-times polynomial is used to preprocess the original data, and the three-classification strategy is used to improve the logistic regression algorithm to predict the dolomite strength. Finally, the significant order of the influence of fracture characteristics on rock strength is judged by numerical simulation software PFC3D, and the dip angle effect of rock fracture is explained from the perspective of fracture propagation. The results show that: 1) When the regularization coefficient λ = 10000, the algorithm has the highest prediction accuracy and the strongest model generalization ability. 2) The numerical simulation analysis software PFC3D can accurately invert rock failure process and characteristics, and the order of influence of fracture characteristics on rock strength is inclination > length > position. 3) When α = 0° ~ 90°, the peak strength decreases first and then increases. The greater the peak strength of the rock, the more obvious the crushing phenomenon, and the worse the wing fracture expansion ability. When α = 60°, the peak strength is the lowest.
Grass-legume intercropping systems in rotation with soybean crops: C and N stocks and CO 2 and NH 3 emissions
Authors: Pinaffi Camila Dias , Moreira Ana Carolina Mônico , Mignacca Flávia Alessandra , Tiritan Carlos Sérgio , Santos Carlos Henrique dos 
Published: March 2023

Abstract
Understanding factors that contribute to carbon dioxide (CO2) losses and ammonia (NH3) volatilization in agricultural soils, mainly in sandy soils, is essential for determining strategies to decrease emissions of these gases and improving carbon (C) and nitrogen (N) stocks in the soil. The objective of this study was to quantify C and N inputs to the soil and CO2 and NH3 emissions in a crop-livestock integration system composed of pastures with grass-legume intercropping and N fertilizer application. The experiment was carried out in a Typic Hapludox, evaluating three consecutive soybean crops grown in rotation with the following pasture systems: mombaca grass (Panicum maximum cv. Mombaca) with N fertilizer (M + N); mombaca grass without N fertilizer (M-N); mombaca grass intercropped with the forage legume pigeon pea (Cajanus cajan) (M + G); and mombaca grass intercropped with the forage legume java (Macrotyloma axillare cv. Java) (M + J). Soil samples were collected at the beginning and end of each crop and analyzed for total C (TCS) and N (TNS) stocks. NH3 volatilization and CO2 emissions were evaluated every 15 days, with simultaneous evaluations of soil moisture and temperature. Soybean rotation with grass-legume intercropping or grass with mineral fertilizer application improved N and C inputs to the agricultural systems, although there were losses in CO2 and NH3. The M + G and M + J intercrops after soybean crops increased NH3 volatilization, whereas the use of M + N tended to decrease it. The soil moisture affected the C and N input-output balance in the production systems evaluated.
The potential of soils from cropland and varying riparian buffer vegetations to emit NO, N2O, N2, and CO2 under denitrification.
Authors: Dlamini Jerry C. , Tesfamariam E. H. , Verbeeck M. , Loick N. , Louro-Lopez A. , Hawkins J. M.B. , Blackwell M. S.A. , Dunn R. , Collins A. L. , Cardenas L. M. 
Published: March 2023

Abstract
Riparian buffers are expedient interventions for water quality functions in agricultural landscapes. However, the choice of their vegetation and management affects soil microbial communities, which in turn affect nutrient cycling and the production and emission of gases such as nitric oxide (NO), nitrous oxide (N2O), nitrogen gas (N2), and carbon dioxide (CO2). To investigate the potential fluxes of the above-mentioned gases, soil samples were collected from a cropland and downslope grass, willow, and woodland riparian buffers from a replicated plot scale experimental facility. The soils were re-packed into cores, and to investigate their potential to produce the aforementioned gases, a potassium nitrate (KNO3−) and glucose (labile carbon)-containing amendment, was added prior to incubation in a specialized laboratory DENItrification System (DENIS). The resulting NO, N2O, N2, and CO2 emissions were measured simultaneously, with the highest NO (2.9 ± 0.31 mg NO m− 2), and N2O (1413.4 ± 448.3 mg N2O m− 2) generated by the grass riparian buffer and the highest N2 (698.1 ± 270.3 mg N2 m− 2) and CO2 (27558.3 ± 128.9 mg CO2 m− 2) produced by the willow riparian buffer. Thus, the results show that soils developed under grass and willow riparian buffers may potentially increase greenhouse gas fluxes, especially in areas with similar conditions to the current study.
Autonomic Neuropathy is Associated with More Densely Interconnected Cytokine Networks in People with HIV
Authors: Lawrence Steven , Mueller Bridget R. , Benn Emma K. T. , Kim-Schulze Seunghee , Kwon Patrick , Robinson-Papp Jessica 
Published: March 2023

Abstract
Introduction. The autonomic nervous system (ANS) plays a complex role in the regulation of the immune system, with generally inhibitory effects via activation of β-adrenergic receptors on immune cells. We hypothesized that HIV-associated autonomic neuropathy (HIV-AN) would result in immune hyperresponsiveness which could be depicted using network analyses.
Methods. Forty-two adults with well-controlled HIV underwent autonomic testing to yield the Composite Autonomic Severity Score (CASS). The observed range of CASS was 2-5, consistent with normal to moderate HIV-AN. To construct the networks, participants were divided into 4 groups based on the CASS (i.e., 2, 3, 4 or 5). Forty-four blood-based immune markers were included as nodes in all networks and the connections (i.e., edges) between pairs of nodes were determined by their bivariate Spearman’s Rank Correlation Coefficient. Four centrality measures (strength, closeness, betweenness and expected influence) were calculated for each node in each network. The median value of each centrality measure across all nodes in each network was calculated as a quantitative representation of network complexity.
Results. Graphical representation of the four networks revealed greater complexity with increasing HIV-AN severity. This was confirmed by significant differences in the median value of all four centrality measures across the networks (p≤0.025 for each).
Conclusion. Among people with HIV, HIV-AN is associated with stronger and more numerous positive correlations between blood-based immune markers. Findings from this secondary analysis can be used to generate hypotheses for future studies investigating HIV-AN as a mechanism contributing to the chronic immune activation observed in HIV.
Vitamin D a panacea compound for silica induced lung oxidative stress and inflammation in Rat
Authors: Mehri Fereshteh , Rahbar Amir Hossein , Asl Sara Soleimani , Esfahani Maryam 
Published: March 2023

Abstract
Introduction: Chronic exposure to silica is related with the provocation of an inflammatory response and oxidative stress mechanism. Vitamin D has multiple benefits in biological activities particularly respiratory system disease.
Methods: In this research, 20 male Wistar rats were randomly allocated into four groups (5 rats /group) as follow: Group1 received saline as (negative control) group. The group 2 received a single IT instillation of silica (positive control) group; the group 3 was co-administrated with single IT silica and Vitamin D (20 mg/kg/day) daily for a period of 90 days. The rats of group 4 received Vitamin D daily for a period of 90 days.
Results: Silica significantly increased serum and lung total Oxidant Status (TOS). Meanwhile, silica reduced serum and lung total antioxidant capacity (TAC), GSH and tumor necrosis factor-α (TNF-a). Vitamin D treatment meaningfully reversed oxidative stress, antioxidants status and inflammatory response. Also, Vitamin D improved histopathological changes caused by silica.
Conclusions: These findings indicate that Vitamin D exerts protective effects against silica-induced lung injury. It seems that Vitamin D has potential use as a therapeutic object for silica induced lung injure
Use of multivariate analysis to identify phytoplankton bioindicators of stream water quality in the mono-modal equatorial agro-ecological zone of Cameroon
Authors: Fai Patricia Bi Asanga , Kenko Daniel Brice Nkontcheu , Tchamadeu Norbert Ngameni , Mbida Mpoame , Korejs Krystof , Riegert Jan 
Published: March 2023

Abstract
The aquatic ecosystem is compromised by many contaminants that may lead to reduced ecosystem functions and severe consequences on human health. This study aimed at determining suitable bioindicators of water quality among the phytoplankton community for the biomonitoring of freshwater streams in the mono-modal agroecological zone of Cameroon. For this purpose, water physicochemical and hydrological parameters, together with the phytoplankton abundance and diversity were measured from June 2016 to May 2017 along the Benoe stream (South-West Region). The Shannon–Wiener diversity and Pielou’s evenness indices indicated a clean to mildly polluted stream with a diverse phytoplankton community which consisted of 84 species belonging to 51 families. The community was dominated by the Bacillariophyta (64%), followed by Chlorophyta (13%) and Cyanophyta (10%). Multivariate statistical analysis revealed that the total dissolved solids (TDS), electrical conductivity (EC), stream water velocity and discharge were the most important stream characteristics affecting the abundance of the dominant phytoplankton genera. These analyses unveiled seasonal variations in the stream characteristics as well as spatial community distribution along an urban - small-scale farming – large-scale farming gradient and their influence on the phytoplankton relative abundances. Consequently, phytoplankton indicator species were identified with increased abundance of Synedra ulna being indicative of low TDS and EC, while Gyrosigma baltium dominance was connected with increased TDS and EC. High Pleurosira laevis abundance was associated with the urban zone while high Diatoma sp. and Oscillatoria sp. abundances were related to large-scale farming zone of the stream. These phytoplankton species have good potential for use as bioindicators for monitoring water quality in the Benoe stream and other streams in the monomodal agroecological zone of Cameroon.
KRASG12C inhibition using MRTX1257: a novel radio-sensitizing partner
Authors: Laurent Pierre-Antoine , Milic Marina , Quevrin Clément , Meziani Lydia , Liu Winchygn , Morel Daphné , Signolle Nicolas , Clémenson Céline , Levy Antonin , Mondini Michele , Deutsch Eric 
Published: March 2023

Abstract
Background
KRAS activating mutations are considered the most frequent oncogenic drivers and are correlated with radio-resistance in multiple cancers including non-small cell lung cancer (NSCLC) and colorectal cancer. Although KRAS was considered undruggable until recently, several KRAS inhibitors have recently reached clinical development. Among them, MRTX849 (Mirati Therapeutics) showed encouraging clinical outcomes for the treatment of selected patients with KRASG12C mutated NSCLC and colorectal cancers. In this work, we explore the ability of MRTX1257, a KRASG12C inhibitor analogous to MRTX849, to radio-sensitize KRASG12C+/+ mutated cell lines and tumors.
Methods
Both in vitro and in vivo models of radiotherapy (RT) in association with MRTX1257 were used, with different RAS mutational profiles. We assessed in vitro the radio-sensitizing effect of MRTX1257 in CT26 KRASG12C+/+, CT26 WT, LL2 WT and LL2 NRAS KO (LL2 NRAS−/−) cell lines. In vivo, we used syngeneic models of subcutaneous CT26 KRASG12C+/+ tumors in BALB/c mice and T cell deficient athymic nu/nu mice to assess both the radio-sensitizing effect of MRTX1257 and its immunological features.
Results
MRTX1257 was able to radio-sensitize CT26 KRASG12C+/+ cells in vitro in a time and dose dependent manner. Moreover, RT in association with MRTX1257 in BALB/c mice bearing CT26 KRASG12C+/+ subcutaneous tumors resulted in an observable cure rate of 20%. However, no durable response was observed with similar treatment in athymic nude mice. The analysis of the immune microenvironment of CT26 KRASG12C+/+ tumors following RT and MRTX1257 showed an increase in the proportion of various cell subtypes including conventional CD4 + T cells, dendritic cells type 2 (cDC2) and inflammatory monocytes. Furthermore, the expression of PD-L1 was dramatically down-regulated within both tumor and myeloid cells, thus illustrating the polarization of the tumor microenvironment towards a pro-inflammatory and anti-tumor phenotype following the combined treatment.
Conclusion
This work is the first to demonstrate in vitro as in vivo the radio-sensitizing effect of MRTX1257, a potent KRASG12C inhibitor compatible with oral administration, in CT26 KRASG12C mutated cell lines and tumors. This is a first step towards the use of new combinatorial strategies using KRAS inhibitors and RT in KRASG12C mutated tumors, which are the most represented in NSCLC with 14% of patients harboring this mutational profile.
Novel dimethylsulfoniopropionate biosynthesis enzymes in diverse marine bacteria, cyanobacteria and abundant algae
Authors: Wang Jinyan , Zhou Shun , Curson Andrew , Vieira Ana , Walsham Keanu , Monaco Serena , Li Chun-Yang , Rivera Peter Paolo , Wang Xiao-Di , Hanwell Libby , Zhu Xiao-Yu , Leão Pedro , Lea-Smith David J. , Zhang Yuzhong , Zhang Xiaohua , Todd Jonathan 
Published: March 2023

Abstract

Dimethylsulfoniopropionate (DMSP) is an abundant marine organosulfur compound[1] with roles in stress protection[2, 3], chemotaxis[4], nutrient and sulfur cycling[5] and, potentially, climate regulation[6, 7]. Marine algae and bacteria are considered significant DMSP producers, but many diverse representatives lack known DMSP synthesis genes/enzymes[8, 9]. Here, new DMSP biosynthesis enzymes were identified that considerably increase the number and diversity of potential DMSP-producing organisms, inferring new and significant global DMSP producers. A novel bifunctional DMSP biosynthesis enzyme, DsyGD, identified in the rhizobacterium Gynuella sunshinyii, produces DMSP at levels higher than any other bacterium from methylthiohydroxybutyrate (MTHB) via an N-terminal MTHB S-methyltransferase domain (termed DsyG) and a C-terminal dimethylsulfoniohydroxybutyrate (DMSHB) decarboxylase domain (termed DsyD, which is the first reported enzyme with this activity). DsyGD is also found in some filamentous cyanobacteria, not previously known to produce DMSP. Regulation of DMSP production and dsyGD transcription was consistent with their role in osmoprotection. Indeed, cloned dsyGD conferred osmotolerance to bacteria deficient in osmolyte production, something not previously demonstrated for any known DMSP synthesis gene, and which could be exploited for biotechnology e.g., engineering salt tolerance. DsyGD characterisation led to identification of phylogenetically distinct DsyG-like proteins, termed DSYE, with MTHB S-methyltransferase activity, in diverse and environmentally abundant Chlorophyta, Chlorachniophyta, Ochraphyta, Haptophyta and Bacillariophyta algae. These algae comprise a mix of low, high and previously unknown DMSP producers[10]. Algae containing DSYE, particularly bloom-forming Pelagophyceae species, which we showed to accumulate medium-high intracellular DMSP levels, were globally more abundant DMSP producers than Haptophyta, Dinophyta and Bacillariophyta with DSYB and/or TpMMT. This highlights the potential importance of Pelagophyceae and other DSYE containing algae in global DMSP production and sulfur cycling.
The rising global economic costs of Aedes and Aedes-borne diseases
Authors: Roiz David , Pontifes Paulina , Jourdain Fréderic , Diagne Christophe , Leroy Boris , Vaissière Anne-Charlotte , Tolsá Maria Jose , Salles Jean-Michel , Simard Frédéric , Courchamp Franck 
Published: March 2023

Abstract
Aedes-borne diseases, such as dengue, chikungunya and Zika are caused by viruses transmitted by Aedes aegypti and Aedes albopictus, producing an enormous public health burden and a lesser-known economic impact. We present a reliable, up-to-date, standardized and easily updatable global-scale synthesis of studies reporting the economic costs caused by these mosquitoes, obtained from 880 records from 118 countries covering the last 45 years. From 1975 to 2020, the cumulative reported cost of Aedes and Aedes-borne diseases was at least 87.3 billion in 2022 US$ values, having increased almost 100-fold over this period, while still considerably underreported and underestimated. Costs are mainly due to dengue (80%), and do not consider the medical sequelae of chikungunya and Zika, which would bring this estimate up to US$ 310.8 billion worldwide. Damage and losses were an order of magnitude higher than investment in management, and only a modest portion of the total reported expenditure went to prevention. Effective control measures are urgently needed to safeguard global health and well-being, and to reduce the burden on human societies. Providing up-to-date data fills a gap in our limited understanding, can inform evidence-based policy and contributes towards a greater awareness of the increasing economic costs of Aedes and Aedes-borne diseases.
The Role of Pyruvate-Induced Enhancement of Oxygen Metabolism in Extracellular Purinergic Signaling in The Post- Cardiac Arrest Rat Model
Authors: Shinozaki Koichiro , Wong Vanessa , Aoki Tomoaki , Hayashida Kei , Takegawa Ryosuke , Endo Yusuke , Nandurkar Harshal , Diamond Betty , Robson Simon C. , Becker Lance B. 
Published: March 2023

Abstract
Purine nucleotide adenosine triphosphate (ATP) is a source of intracellular energy maintained by mitochondrial oxidative phosphorylation. However, when released from ischemic cells into the extracellular space, they act as death-signaling molecules (eATP). Despite there being potential benefit in using pyruvate to enhance mitochondria by inducing a highly oxidative metabolic state, its association with eATP levels is still poorly understood. Therefore, while we hypothesized that pyruvate could beneficially increase intracellular ATP with the enhancement of mitochondrial function after cardiac arrest (CA), our main focus was whether a proportion of the raised intracellular ATP would detrimentally leak out into the extracellular space. Indicated by the increased levels in systemic oxygen consumption and brain ATP levels, intravenous administrations of bolus (500 mg/kg) and continuous infusion (1000 mg/kg/hr) of pyruvate successfully increased oxygen and energy metabolism in post 10-min CA rats. The plasma ATP levels increased significantly from 67 ± 11 nM before CA to 227 ± 100 nM 2 hours after the resuscitation, while the pyruvate injection did not affect post-CA ATP levels. Notably, the pyruvate injection improved post-CA cardiac contraction and acidemia (low pH). We also found that pyruvate increased systemic CO2 production post-CA. These data support that pyruvate has therapeutic potential for improving CA outcomes by enhancing oxygen and energy metabolism in the brain and heart, and attenuating intracellular hydrogen iron disorders, but does not exacerbate the death-signaling of eATP in the blood.
Design optimization for high-performance computing using FPGA
Authors: Isik Murat , Inadagbo Kayode , Aktas Hakan 
Published: March 2023

Abstract
Reconfigurable architectures like Field Programmable Gate Arrays (FPGAs) have been used for accelerating computations in several domains because of their unique combination of flexibility, performance, and power efficiency. However, FPGAs have not been widely used for high-performance computing, primarily because of their programming complexity and difficulties in optimizing performance. We optimize Tensil AI's open-source inference accelerator for maximum performance using ResNet20 trained on CIFAR in this paper in order to gain insight into the use of FPGAs for high-performance computing. In this paper, we show how improving hardware design, using Xilinx Ultra RAM, and using advanced compiler strategies can lead to improved inference performance. We also demonstrate that running the CIFAR test data set shows very little accuracy drop when rounding down from the original 32-bit floating point. The heterogeneous computing model in our platform allows us to achieve a frame rate of 293.58 frames per second (FPS) and a \%90 accuracy on a ResNet20 trained using CIFAR. The experimental results show that the proposed accelerator achieves a throughput of 21.12 Giga-Operations Per Second (GOP/s) with a 5.21 W on-chip power consumption at 100 MHz. The comparison results with off-the-shelf devices and recent state-of-the-art implementations illustrate that the proposed accelerator has obvious advantages in terms of energy efficiency.