bluuebunny/crossref_metadata_2025_split huggingface dataset:
Lake Sinai virus is a diverse, globally distributed but not emerging multi‐strain honeybee virus
Authors: Hou Chunsheng , Liang Hao , Chen Chenxiao , Zhao Hongxia , Zhao Pengfei , Deng Shuai , Li Beibei , Yang Dahe , Yang Sa , Wilfert Lena 
Published: May 2023

AbstractDomesticated honeybees and wild bees are some of the most important beneficial insects for human and environmental health, but infectious diseases pose a serious risk to these pollinators, particularly following the emergence of the ectoparasitic mite Varroa destructor as a viral vector. The acquisition of this novel viral vector from the Asian honeybee Apis ceranae has fundamentally changed viral epidemiology in its new host, the western honeybee A. mellifera. While the recently discovered Lake Sinai Viruses (LSV) have been associated with weak honeybee colonies, they have not been associated with vector‐borne transmission. By combining a large‐scale multi‐year survey of LSV in Chinese A. mellifera and A. cerana honeybee colonies with globally available LSV‐sequence data, we investigate the global epidemiology of this virus. We find that globally distributed LSV is a highly diverse multi‐strain virus, which is predominantly associated with the western honeybee A. mellifera. In contrast to the vector‐borne deformed wing virus, LSV is not an emerging disease. Instead, demographic reconstruction and strong global and local population structure indicates that it is a highly variable multi‐strain virus in a stable association with its main host, the western honeybee. Prevalence patterns in China suggest a potential role for migratory beekeeping in the spread of this pathogen, demonstrating the potential for disease transmission with the man‐made transport of beneficial insects.
The role of gene presence–absence variations on genetic incompatibility in Asian rice
Authors: Li Juan , Lee Cheng‐Ruei 
Published: May 2023

Summary
Genetic incompatibilities are widespread between species. However, it remains unclear whether they all originated after population divergence as suggested by the Bateson–Dobzhansky–Muller model, and if not, what is their prevalence and distribution within populations.
The gene presence–absence variations (PAVs) provide an opportunity for investigating gene–gene incompatibility. Here, we searched for the repulsion of coexistence between gene PAVs to identify the negative interaction of gene functions separately in two Oryza sativa subspecies.
Many PAVs are involved in subspecies‐specific negative epistasis and segregate at low‐to‐intermediate frequencies in focal subspecies but at low or high frequencies in the other subspecies. Incompatible PAVs are enriched in two functional groups, defense response and protein phosphorylation, which are associated with plant immunity and consistent with autoimmunity being a known mechanism of hybrid incompatibility in plants. Genes in the two enriched functional groups are older and seldom directly interact with each other. Instead, they interact with other younger gene PAVs with diverse functions.
Our results illustrate the landscape of genetic incompatibility at gene PAVs in rice, where many incompatible pairs have already segregated as polymorphisms within subspecies, and many are novel negative interactions between older defense‐related genes and younger genes with diverse functions.
Xanthophyll esterases in association with fibrillins control the stable storage of carotenoids in yellow flowers of rapeseed (Brassica juncea)
Authors: Li Rihui , Zeng Qinyu , Zhang Xiangxiang , Jing Jing , Ge Xiaoyu , Zhao Lun , Yi Bin , Tu Jinxing , Fu Tingdong , Wen Jing , Shen Jinxiong 
Published: May 2023

Summary
Biosynthesis, stabilization, and storage of carotenoids are vital processes in plants that collectively contribute to the vibrant colors observed in flowers and fruits. Despite its importance, the carotenoid storage pathway remains poorly understood and lacks thorough characterization.
We identified two homologous genes, BjA02.PC1 and BjB04.PC2, belonging to the esterase/lipase/thioesterase (ELT) family of acyltransferases. We showed that BjPCs in association with fibrillin gene BjFBN1b control the stable storage of carotenoids in yellow flowers of Brassica juncea.
Through genetic, high‐resolution mass spectrometry and transmission electron microscopy analyses, we demonstrated that both BjA02.PC1 and BjB04.PC2 can promote the accumulation of esterified xanthophylls, facilitating the formation of carotenoid‐enriched plastoglobules (PGs) and ultimately producing yellow pigments in flowers. The elimination of BjPCs led to the redirection of metabolic flux from xanthophyll ester biosynthesis to lipid biosynthesis, resulting in white flowers for B. juncea. Moreover, we genetically verified the function of two fibrillin genes, BjA01.FBN1b and BjB05.FBN1b, in mediating PG formation and demonstrated that xanthophyll esters must be deposited in PGs for stable storage.
These findings identified a previously unknown carotenoid storage pathway that is regulated by BjPCs and BjFBN1b, while offering unique opportunities for improving the stability, deposition, and bioavailability of carotenoids.
Superior vena cava tear during transvenous lead extraction: Medical management in hemodynamically stable patients
Authors: Domain Guillaume , Strubé Camille , Jacques Frédéric , Marzouk Mohamed , Dumont Éric , Villeneuve Jacques , Plourde Benoît , Albert Gabriella , Sarrazin Jean‐François , Steinberg Christian , Philippon François 
Published: May 2023

AbstractIntroductionSuperior vena cava (SVC) tear is the most lethal complication during transvenous lead extraction (TLE) with a mortality rate as high as 50%. Treatment involves aggressive attempts to maintain cardiac output and immediate sternotomy to localize and repair the vascular tear. Occlusion balloons have been developed to provisionally occlude the lacerated SVC and to provide hemodynamic stability allowing time for surgery. In case of mediastinal hematoma without hemodynamic instability, the strategy remains unclear.Methods and ResultsWe describe two cases of SVC tear during TLE. The first case was a 60‐year‐old man who presented with a right ventricular single‐chamber defibrillator lead fracture and innominate vein stenosis. The RV lead was removed using a laser sheath causing a mediastinal hematoma with no active bleeding during surgical exploration few hours later. The second case was a 28‐year‐old man that presented with a right atrial (RA) lead fracture and RV lead insulation failure in a dual‐chamber defibrillator (ICD).ConclusionBoth the RA and RV leads were removed with mechanical sheaths, and a mediastinal hematoma was medically managed.
Don't just tell me, show me: Impacting perceptions of organizational attraction and fit using activating LGBT diversity signals
Authors: Bradley Cody , Moergen Kristie J. N. , Roumpi Dorothea , Simon Lauren S. 
Published: May 2023

AbstractTo appeal to traditionally hard‐to‐reach populations such as the LGBT community, many organizations have made diversity and inclusion a focal point of their recruitment messaging. However, extant research provides little evidence of the effectiveness of this messaging for its intended and non‐intended targets. Building upon a proposed signaling theory typology, we suggest that activating signals—signals that show how diversity and inclusion are actively embodied or “activated” through organizational practices—are more effective than pointing signals—signals that merely tell an individual that diversity and inclusion is valued. After validating a collection of individual diversity signals using this typology, we test our theory across multiple studies. In Study 1, we focus specifically on LGBT job seekers and conduct an experiment to examine the degree to which various LGBT diversity signals on a fictional company website influence initial assessments of organizational attraction and person‐organization fit. In Study 2, we replicate and extend Study 1 by demonstrating how the positive effects of LGBT diversity signals are similar in a sample of non‐LGBT job seekers. Finally, in Study 3, we extend the generalizability of our theorizing using a sample of recent job seekers and real organizational websites.
Successful transplantation of four kidney grafts from two small pediatric donors with anuric acute renal failure into adult recipients
Authors: Koch Martina , Kraus Daniel , Boedecker‐Lips Simone , Mittler Jens , Tieken Ineke , Weinmann‐Menke Julia 
Published: May 2023

AbstractBackgroundKidneys from infants with anuric acute kidney injury (AKI) only rarely get accepted for transplantation despite encouraging data that such kidneys can have very good long‐term outcome.MethodsWe report the transplantation of four kidney grafts from two pediatric donors (3 and 4 years) with anuric acute kidney injury as single kidneys into four adult recipients.ResultsAll grafts gained function within 14 days posttransplantation, only one recipient needed dialysis after transplantation. None of the recipients suffered from surgical complications. One month after transplantation, all recipients were free of dialysis. Estimated glomerular filtration rates (eGFR) 3 months after transplantation were 37, 40, 50, and 83 mL/min/1.73 m2. eGFR increased further through month 6, reaching 45, 50, 58, and 89 mL/min/1.73 m2.ConclusionThese cases highlight the feasibility of successful transplantation of single pediatric kidney grafts into adult recipients despite anuric AKI of the donor.
Shifts in the portfolio holdings of euro area investors in the midst of COVID‐19: Looking‐through investment funds
Authors: Carvalho Daniel , Schmitz Martin 
Published: May 2023

AbstractWe study the impact of the COVID‐19 shock on the portfolio exposures of euro area investors. The analysis “looks‐through” holdings of investment fund shares to first gauge euro area investors' full exposures to global debt securities and listed shares by sector at end‐2019 and to subsequently analyse the portfolio shifts in the first and second quarters of 2020. We show heterogeneous patterns across asset classes and sectors, but also across less and more vulnerable euro area countries. In particular, we find a broad‐based rebalancing towards domestic sovereign debt at the expense of extra‐euro area sovereigns in the first quarter of 2020, consistent with heightened home bias, which however levelled off in the second quarter. On the contrary, for listed shares we find that euro area investors rebalanced away from domestic towards extra‐euro area securities in both the first and the second quarter, which may be associated with better relative foreign stock market performance. Many of these shifts were only due to indirect holdings, corroborating the importance of investment funds in assessing investors' exposures—especially for households, insurance companies and pension funds—in particular in times of large shocks. We also confirm the important intermediation role played by investment funds in an analysis focusing on the large‐scale portfolio rebalancing observed between 2015 and 2017 during the ECB's Asset Purchase Programme.
COVID‐19 lockdown anxiety harms newcomers' job satisfaction: A cross‐lagged panel analysis during snap lockdowns in China
Authors: Liu Su , Zheng Lu , Scott English Alexander , Talhelm Thomas , Sun Junjie , Xu Shuhong 
Published: May 2023

AbstractThe COVID‐19 pandemic may have been a difficult time to join a new organization. Drawing on the feelings‐as‐information theory, this study explores how COVID‐19 lockdown anxiety influenced newcomers' job satisfaction during their first few months of work. We tested 357 new employees working in 84 cities across China. We conducted a longitudinal study, and participants were invited to complete the same survey at two time points. Cross‐lagged panel analysis was conducted to test our hypotheses. We confirmed that COVID‐19 lockdown anxiety at Time 1 predicted less job satisfaction at Time 2, whereas the data did not support the idea of reverse causality. These findings suggest public health crises like the pandemic can impact newcomers' job satisfaction, especially during China's Zero‐Covid Policy.
Care, lead, and inspire: Infusing innovation into nursing research and evidence‐based practice course
Authors: Catarelli Bryce , Nobles Patrick , Scarton Lisa , Duckworth Laurie , Ezenwa Miriam O. , Booker Staja Q. 
Published: May 2023

AbstractBackgroundToday's nursing workforce is expected to know how to identify and understand research methods and procedures and apply the most current evidence into daily practice. However, teaching evidence‐based practice (EBP) in an undergraduate nursing curriculum poses unique challenges in overcoming students' perception of content relevancy to their educational experience, but also offers opportunities for innovation to facilitate critical thinking and clinical application.AimsThe aim of this article is to report on how teaching and learning innovation was infused into a research and evidence‐based practice course and the effect on students' perceptions of course values and effectiveness.MethodsWe used a Plan‐Do‐Study‐Act approach to introduce innovation in an undergraduate course within a university setting. Final student course evaluations were used to measure outcomes on a 5‐point Likert scale (1 = low and 5 = high) on the following dimensions: (1) value of overall educational experience, (2) relevancy of course content, (3) improvement in critical thinking, and (4) level of student‐instructor interaction.ResultsOverall course evaluation scores improved greatly from 2.69 to 3.90 between Spring 2020 and Fall 2021. This finding remained relatively consistent across subsequent semesters (3.79 [Spring 2022], 3.84 [Fall 2022]). Students also reported appreciation and increased engagement and interest with the material after transitioning from examinations to a project‐based assignment that allowed them to walk through the steps of EBP in class.Linking Evidence to ActionWe identified and implemented several innovative strategies to improve student outcomes and increase the relevance of the course content. These innovations can be easily incorporated at other universities to enhance delivery and student engagement in this content that is essential to advancing quality care in nursing and developing future nurse scientists and practice leaders who care, lead, and inspire.
Modulation of dorsal premotor cortex differentially influences I‐wave excitability in primary motor cortex of young and older adults
Authors: Liao Wei‐Yeh , Opie George M. , Ziemann Ulf , Semmler John G. 
Published: May 2023

AbstractPrevious research using transcranial magnetic stimulation (TMS) has demonstrated weakened connectivity between dorsal premotor cortex (PMd) and motor cortex (M1) with age. While this alteration is probably mediated by changes in the communication between the two regions, the effect of age on the influence of PMd on specific indirect (I) wave circuits within M1 remains unclear. The present study therefore investigated the influence of PMd on early and late I‐wave excitability in M1 of young and older adults. Twenty‐two young (mean ± SD, 22.9 ± 2.9 years) and 20 older (66.6 ± 4.2 years) adults participated in two experimental sessions involving either intermittent theta burst stimulation (iTBS) or sham stimulation over PMd. Changes within M1 following the intervention were assessed with motor‐evoked potentials (MEPs) recorded from the right first dorsal interosseous muscle. We applied posterior–anterior (PA) and anterior–posterior (AP) current single‐pulse TMS to assess corticospinal excitability (PA1mV; AP1mV; PA0.5mV, early; AP0.5mV, late), and paired‐pulse TMS short intracortical facilitation for I‐wave excitability (PA SICF, early; AP SICF, late). Although PMd iTBS potentiated PA1mV and AP1mV MEPs in both age groups (both P < 0.05), the time course of this effect was delayed for AP1mV in older adults (P = 0.001). Furthermore, while AP0.5mV, PA SICF and AP SICF were potentiated in both groups (all P < 0.05), potentiation of PA0.5mV was only apparent in young adults (P < 0.0001). While PMd influences early and late I‐wave excitability in young adults, direct PMd modulation of the early circuits is specifically reduced in older adults.
imageKey points
Interneuronal circuits responsible for late I‐waves within primary motor cortex (M1) mediate projections from dorsal premotor cortex (PMd), but this communication probably changes with advancing age.
We investigated the effects of intermittent theta burst stimulation (iTBS) to PMd on transcranial magnetic stimulation (TMS) measures of M1 excitability in young and older adults.
We found that PMd iTBS facilitated M1 excitability assessed with posterior–anterior (PA, early I‐waves) and anterior–posterior (AP, late I‐waves) current TMS in young adults, with a stronger effect for AP TMS.
M1 excitability assessed with AP TMS also increased in older adults following PMd iTBS, but there was no facilitation for PA TMS responses.
We conclude that changes in M1 excitability following PMd iTBS are specifically reduced for the early I‐waves in older adults, which could be a potential target for interventions that enhance cortical excitability in older adults.
In Silico Mechanical Effort Analysis of the All-On-4 Design Performed With Platform-Switching Distal Short Dental Implants
Authors: Santana Luís Carlos Leal , Idogava Henrique Takashi , De Foggi Camila Cristina , Guastaldi Fernando Pozzi Semeghini , Noritomi Pedro Yoshito , dos Reis Barbara Araújo , Vaz Luis Geraldo 
Published: May 2023

AbstractShort dental implants with platform matching connection have been used for the rehabilitation of atrophic jaws whenever standard-length dental implants cannot be placed without prior bone augmentation. Yet, there remains a lack of data regarding the risk of technical failures when the all-on-4 configuration is performed in atrophic jaws with platform-switching distal short dental implants. Thus, the current study used the finite element method to evaluate the mechanical behavior at the level of the prosthetic components of the all-on-4 concept performed in atrophic mandible using short-length distal implants with platform switching (PSW) connection. Three models of the all-on-4 configuration were generated in human atrophic mandibles. The geometric models consisted of PSW connection tilted standard (AO4T; θ = 30 deg; 11 mm-length), straight standard (AO4S; θ = 0 deg; 11 mm-length) and straight short (AO4Sh; θ = 0 deg; 8 mm-length) distal implants. A resultant force of 300 N was performed obliquely in the left side and posterior region of the prosthetic bar. The von Mises equivalent stress (σvm) and maximum and minimum principal stresses (σmax and σmin) were performed at level of the prosthetic components/implants and peri-implant bone crest, respectively. The general displacement of the models was also evaluated. The stress analysis was performed on the side of load application. The AO4S configuration showed the lowest values of σvm in the mesial left (ML) and distal left (DL) abutments (37.53 MPa and 232.77 MPa, respectively) and dental implants (91.53 MPa and 231.21 MPa, respectively). The AO4Sh configuration showed the highest values of σvm in the bar screw (102.36 MPa), abutment (117.56 MPa), and dental implant (293.73 MPa) of the ML area. Among the models, the highest values of σmax and σmin were noticed in the peri-implant bone crest of the AO4T design (131.48 MPa and 195.31 MPa, respectively). All models showed similar values of general displacements, which were concentrated in the mandible symphysis. The all-on-4 configurations designed with PSW connection and tilted standard (AO4T; θ = 30 deg; 11 mm-length), straight standard (AO4S; θ = 0 deg; 11 mm-length) or straight short (AO4Sh; θ = 0 deg; 8 mm-length) distal implants were not associated with higher odds of technical failures. The AO4Sh design may be a promising option for the prosthetic rehabilitation of atrophic jaws.
An Insight into Quasi-Two-Dimensional Flow Features Over Turbine Blading From the Works of Jonathan Paul Gostelow
Authors: Rona Aldo , Adebayo David S. , Gostelow Jonathan Paul 
Published: May 2023

Abstract
The flow through the predominantly two-dimensional geometries of cascades of blades is intrinsically three-dimensional and unsteady. Direct Numerical Simulation, Large Eddy Simulations, and time-resolved Particle Image Velocimetry provide access to the full flow physics, relevant to aerodynamic loss and heat management. Such studies build upon earlier insight drawn from quasi-two-dimensional investigations that identified the key areas where progress in understanding was most needed. These areas stretch across the full passage, from the leading edge of the blade to the passage outflow. Streamwise surface vorticity, transition, the calmed region, shock–boundary layer interaction, and vortex shedding are considered in detail, specifically (i) on what gaps in their physical understanding the works of Jonathan Paul Gostelow exposed and (ii) what gaps were present in the two-dimensional computational approaches used to represent these flows in these works. These useful insights are obtained from the geometrically simpler settings of circular cylinders in cross-flow and from flat plate experiments, as well as from cascades of blades. This paper presents an overview of the physical understanding of the flow features that underpins the more recent time-resolved three-dimensional investigations, led by the late Emeritus Professor Jonathan Paul Gostelow. This work celebrates some of Paul Gostelow’s 50 + years of turbomachinery research achievements and develops awareness about their significance toward reaching a more complete knowledge of the flow physics in turbomachinery, using the more recent time-resolved three-dimensional modeling capability of Computational Fluid Dynamics.