bluuebunny/crossref_metadata_2025_split huggingface dataset:
A Novel Diagnostic Tool for Human-Centric Quality Monitoring in Human–Robot Collaboration Manufacturing
Authors: Verna Elisa , Puttero Stefano , Genta Gianfranco , Galetto Maurizio 
Published: August 2023

Abstract
The manufacturing industry is currently facing an increasing demand for customized products, leading to a shift from mass production to mass customization. As a result, operators are required to produce multiple product variants with varying complexity levels while maintaining high-quality standards. Further, in line with the human-centered paradigm of Industry 5.0, ensuring the well-being of workers is equally important as production quality. This paper proposes a novel tool, the “Human–Robot Collaboration Quality and Well-Being Assessment Tool” (HRC-QWAT), which combines the analysis of overall defects generated during product variant manufacturing with the evaluation of human well-being in terms of stress response. The HRC-QWAT enables the evaluation and monitoring of human–robot collaboration systems during product variant production from a broader standpoint. A case study of collaborative human–robot assembly is used to demonstrate the applicability of the proposed approach. The results suggest that the HRC-QWAT can evaluate both production quality and human well-being, providing a useful tool for companies to monitor and improve their manufacturing processes. Overall, this paper contributes to developing a human-centric approach to quality monitoring in the context of human–robot collaborative manufacturing.
Strategic Production Process Design With Additive Manufacturing in a Make-to-Stock Environment
Authors: Chua Ping Chong , Moon Seung Ki , Ng Yen Ting , Lopez Manel 
Published: August 2023

Abstract
With the development and gradual maturity of additive manufacturing (AM) over the years, AM has reached a stage where implementation into a conventional production system becomes possible. With AM suitable for small volumes of highly customized production, there are various ways of implementing AM in a conventional production line. The aim of this paper is to present a strategic design approach to implementing AM with conventional manufacturing in a complementary manner for parallel processing of production orders of large quantities in a make-to-stock environment. By assuming that a single machine in conventional manufacturing can be operated using AM, splitting of production orders is allowed. Therefore, production can be conducted by both conventional and AM processes simultaneously, with the latter being able to produce various make-to-stock parts in a single build. A genetic algorithm with a scheduling and rule-based heuristic for part allocation on the build plate of AM process is used to solve a multi-objective implementation problem of AM with conventional manufacturing, with cost, scheduling, and sustainability being the considered performance measures. By obtaining a knee-point solution using varying numbers of population size and generation number, an experiment involving an industry case study of implementing the fused deposition modeling (FDM) process with injection molding process shows the greatest impact, i.e., increase, in cost. Except for material efficiency, improvements are shown in scheduling and carbon footprint objectives.
Unraveling and characterization of novel T3SS effectors in
Edwardsiella piscicida
Authors: Liao Xiao Jian , He Tian Tian , Liu Lu Yi , Jiang Xiu Long , Sun Shan Shan , Deng Yu Hang , Zhang Li Qiang , Xie Hai Xia , Nie Pin 
Published: August 2023

ABSTRACT

Type III secretion system (T3SS) facilitates survival and replication of
Edwardsiella piscicida in vivo
. Identifying novel T3SS effectors and elucidating their functions are critical in understanding the pathogenesis of
E. piscicida. E. piscicida
T3SS effector EseG and EseJ was highly secreted when T3SS gatekeeper-containing protein complex EsaB-EsaL-EsaM was disrupted by EsaB deficiency. Based on this observation, concentrated secretomes of Δ
esaB
strain and Δ
esaB
Δ
esaN
strain were purified by loading them into SDS-PAGE gel for a short electrophoresis to remove impurities prior to the in-the gel digestion and mass spectrometry. Four reported T3SS effectors and two novel T3SS effector candidates EseQ (ETAE_2009) and Trx2 (ETAE_0559) were unraveled by quantitative comparison of the identified peptides. EseQ and Trx2 were revealed to be secreted and translocated in a T3SS-dependent manner through CyaA-based translocation assay and immunofluorescent staining, demonstrating that EseQ and Trx2 are the novel T3SS effectors of
E. piscicida
. Trx2 was found to suppress macrophage apoptosis as revealed by TUNEL staining and cleaved caspase-3 of infected J774A.1 monolayers. Moreover, Trx2 has been shown to inhibit the p65 phosphorylation and p65 translocation into the nucleus, thus blocking the NF-κB pathway. Furthermore, depletion of Trx2 slightly but significantly attenuates
E. piscicida
virulence in a fish infection model. Taken together, an efficient method was established in unraveling T3SS effectors in
E. piscicida
, and Trx2, one of the novel T3SS effectors identified in this study, was demonstrated to suppress apoptosis and block NF- κB pathway during
E. piscicida
infection.

IMPORTANCE

Edwardsiella piscicida
is an intracellular bacterial pathogen that causes intestinal inflammation and hemorrhagic sepsis in fish and human. Virulence depends on the
Edwardsiella
type III secretion system (T3SS). Identifying the bacterial effector proteins secreted by T3SS and defining their role is key to understanding
Edwardsiella
pathogenesis. EsaB depletion disrupts the T3SS gatekeeper-containing protein complex, resulting in increased secretion of T3SS effectors EseG and EseJ. EseQ and Trx2 were shown to be the novel T3SS effectors of
E. piscicida
by a secretome comparison between ∆
esaB
strain and ∆
esaB
∆
esaN
strain (T3SS mutant), together with CyaA-based translocation assay. In addition, Trx2 has been shown to suppress macrophage apoptosis and block the NF-κB pathway. Together, this work expands the known repertoire of T3SS effectors and sheds light on the pathogenic mechanism of
E. piscicida
.
Flagella-related gene mutations in
Vibrio cholerae
during extended cultivation in nutrient-limited media impair cell motility and prolong culturability
Authors: Okada Kazuhisa , Roobthaisong Amonrattana , Hamada Shigeyuki 
Published: August 2023

ABSTRACT

Understanding the survival strategies of pandemic cholera pathogens in aquatic environments is important for preventing their dissemination. Here, we report a phenomenon wherein long-term cultivation of
Vibrio cholerae
under nutrient-limited condition (M9 minimal medium supplemented with 0.2% glucose) causes mutations in flagella-related genes, thus inhibiting motility. Moreover, the motility defect reduced the transition of the viable but nonculturable state of the organisms. Maintenance of proliferative capability allows for the rapid population growth and prolonged survival in environments with nutrient sources. Diverse flagella-related genes, including
flrA, flrC, flrD, flhA, flhF, fliD, fliF, fliG, fliH, fliI, fliP, fliQ, flgF, flgL, flgK, motX,
and
pomA,
underwent mutations during the cultivation. However, only one gene (site) was mutated in a single
V. cholerae
. Longer cultivation for up to 300 days yielded further mutations in metabolism-related genes and the loss of virulence factors (e.g., CTX phage) and large DNA regions (~35 kb). These mutations were detected in most organisms of
V. cholerae
, accompanying the loss of genomic integrity. Motility-defective variants with mutations in the acetate kinase gene had become predominant in culturable cells after long-term cultivation in independently replicated experiments. These findings shed light on a survival mechanism in which
V. cholerae
adapts to an environmental niche by accumulating mutations.

IMPORTANCE

Vibrio cholerae
undergoes a transition to a viable but non-culturable (VNC) state when subjected to various environmental stresses. We showed here that flagellar motility was involved in the development of the VNC state of
V. cholerae
. In this study, motility-defective isolates with mutations in various flagella-related genes, but not motile isolates, were predominantly obtained under the stress of long-term batch culture. Other genomic regions were highly conserved, suggesting that the mutations were selective. During the stationary phase of long-term culture,
V. cholerae
isolates with mutations in the acetate kinase and flagella-related genes were predominant. This study suggests that genes involved in specific functions in
V. cholerae
undergo mutations under certain environmental conditions.
DisseminatedStrongyloides stercoralisinfection in the setting ofEscherichia colimeningitis and bacteraemia in a patient living with HIV on high-dose corticosteroid therapy
Authors: Montreuil Nadine , Sternberg Candice A , Abaribe Obinna , Ayoade Folusakin O 
Published: August 2023

Strongyloidiasis, a helminth infection caused byStrongyloides stercoralis, can be complicated by hyperinfection, especially in the setting of immunosuppression; however, many patients go undiagnosed. One clue to diagnosis is unexplained gram-negative bacteraemia or meningitis in patients who are immunosuppressed. Serology can be helpful but may be negative in these patients who are immunocompromised.We present the case of a white cisgender man from Central America in his 40s, living with HIV, with a CD4 count of 77 cells/µL. He was diagnosed withStrongyloideshyperinfection after an increase in his steroid dose. He also hadEscherichia colimeningitis and bacteraemia. Strongyloidiasis was diagnosed by stool microscopy despite a negative serology test.This case highlights the challenges in diagnosing strongyloidiasis in the setting of immunosuppression. A high index of clinical suspicion is warranted for patients living with HIV on high-dose corticosteroids. Up to three stool microscopy studies forStrongyloidesshould be sent in addition to serology.
Safety and efficacy of Vitamin D3supplementation with Imatinib in Chronic Phase- Chronic Myeloid Leukaemia: an Exploratory Randomized Controlled Trial
Authors: Bandyopadhyay Arkapal , Palepu Sarika , Dhamija Puneet , Nath Uttam Kumar , Chetia Rituparna , Bakliwal Anamika , Vaniyath Sudeep , Chattopadhyay Debranjani , Handu Shailendra 
Published: August 2023

ObjectivesThe study aimed to compare early molecular response (EMR) rates at 3 months of imatinib therapy with and without vitamin D3supplementation in patients newly diagnosed with chronic-phase chronic myeloid leukaemia (CML-CP). The secondary objective was to assess the effects of vitamin D3on complete haematological response (CHR) and its safety.DesignDouble-blind, placebo-controlled, exploratory randomised trial.SettingTertiary care hospital in northern India.ParticipantsTreatment-naive patients with chronic phase chronic myeloid leukaemia (n=62) aged >12 years were recruited from January 2020 to January 2021. Patients with progressive disease, pregnancy and hypercalcaemia were excluded.InterventionOral vitamin D3supplementation (60 000 IU) or matched placebo was given once weekly for an initial 8 weeks along with imatinib after randomisation with 1:1 allocation ratio.Primary and secondary outcome measuresThe primary outcome was to compare EMR (defined asBCR-ABL1transcript level ≤10%, international scale) at 3 months. The secondary outcomes were to compare effect of the intervention on CHR, correlation of 25(OH)2D3levels with treatment response and safety according to Common Terminology Criteria for Adverse Events (CTCAE) version 5.ResultsAt baseline, 14.5% of the patients had normal vitamin D3levels. EMR at 3 months was attained in 24 patients (82.7%) of the vitamin D3group and 21 (75%) of the placebo group (OR 1.6, 95% CI 0.37 to 7.37, p=0.4). A significant difference in vitamin D3levels from baseline to the end of study was observed. Patients with vitamin D3supplementation did not achieve higher CHR in comparison with placebo (OR 1.3, 95% CI 0.25 to 7.23, p=1.0). Vitamin D3 levels were not significantly correlated withBCR-ABL1levels. No dose-limiting toxicities were observed.ConclusionVitamin D3levels were low among patients with CML-CP in this study. Vitamin D3supplementation with imatinib therapy did not have significant effect on EMR or CHR. Further clinical trials could be undertaken to assess the effective dosage and duration of vitamin D3supplementation in these patients.Trial registration numberCTRI/2019/09/021164.
Effect modification in network meta-analyses for relapsed/refractory multiple myeloma: systematic review and meta-analysis
Authors: Rose Christopher James , Ohm Ingrid Kristine , Giske Liv , Næss Gunn Eva , Fretheim Atle 
Published: August 2023

ObjectivesTo systematically review and meta-analyse the evidence for effect modification by refractory status and number of treatment lines in relapsed/refractory multiple myeloma (RRMM); and to assess whether effect modification is likely to invalidate network meta-analyses (NMA) that assume negligible modification.DesignSystematic review, meta-analysis and simulation.Data sourcesWe systematically searched the literature (e.g., OVID Medline) to identify eligible publications in February 2020 and regularly updated the search until January 2022. We also contacted project stakeholders (including industry)Eligibility criteriaPhase 2 and 3 randomised controlled trials reporting stratified estimates for comparisons with at least one of a prespecified set of treatments relevant for use in Norwegian RRMM patients.OutcomesWe used meta-analysis to estimate relative HRs (RHRs) for overall survival (OS) and progression-free survival (PFS) with respect to refractory status and number of treatment lines. We used the estimated RHRs in simulations to estimate the percentage of NMA results expected to differ significantly in the presence versus absence of effect modification.ResultsAmong the 42 included publications, stratified estimates were published by and extracted from up to 18 (43%) publications and on as many as 8364 patients. Within-study evidence for effect modification is very weak (p>0.05 for 47 of 49 sets of stratified estimates). The largest RHR estimated was 1.32 (95% CI 1.18 to 1.49) for the modifying effect of refractory status on HR for PFS. Simulations suggest that, in the worst case, this would result in only 4.48% (95% CI 4.42% to 4.54%) of NMA estimates differing statistically significantly in the presence versus absence of effect modification.ConclusionsBased on the available evidence, effect modification appears to be sufficiently small that it can be neglected in adequately performed NMAs. NMAs can probably be relied on to provide estimates of HRs for OS and PFS in RRMM, subject to caveats discussed herein.
Scoping review on Physical Health Conditions in Irish Travellers (Mincéiri)
Authors: Kennedy Fiona , Ward Amy , Mockler David , Villani Jacopo , Broderick Julie 
Published: August 2023

ObjectiveThe objective of this scoping review was to collate physical health conditions in Mincéiri—Irish Travellers.DesignScoping review.Search strategy and charting methodMEDLINE/PubMed, EMBASE, PEDro, AMED, CINAHL, PsycINFO, SCOPUS as well as reports and grey literature were searched for primary data reporting physical health conditions of Irish Travellers up to 4 April 2023. Data was extracted, described and organised meaningfully into tables according to reported physical health conditions.Eligibility criteriaThe population was Travellers. The concept referred to physical health conditions. The context was Irish Travellers based in any location or setting. Exclusion criteria was data/research other than primary data relating to physical health conditions of Irish Travellers.ResultsFrom 198 citations generated from the database search, 11 unique studies (20 reports) were included in this scoping review, including n=7397 participants. Driven by the data, physical health conditions were categorised into cardiovascular diseases, respiratory diseases, injuries/musculoskeletal/arthritic disorders, genetic disorders and gut/bowel conditions. This review showed that the metabolic syndrome, asthma, bronchitis, tuberculosis and intentional injuries were 2–3 times more prevalent in Irish Travellers compared with the background population. Genetic conditions were also described in a proportion of Travellers.ConclusionsOverall, Irish Travellers experience a disproportionate burden of physical health conditions compared with background populations. Healthcare providers need to be aware of the unique physical health burden experienced by many Irish Travellers. Multifaceted strategies are needed to improve the health profile of this vulnerable and marginalised group.
Improving primary care referral to specialist services: a protocol for a 10-year global systematic review in the Australian context
Authors: Ekanayake Siyaguna Kosgamage Dilum Manthinda , Ward Aletha E , Heart Diane , Valery Patricia , Soar Jeffrey 
Published: August 2023

IntroductionBarriers to accessing specialist services impart a significant burden on patient outcomes and experience as well as a cost and administrative burden on health systems due to healthcare wastage and inefficiencies. This paper outlines the planned protocol for a systematic review relating to how health systems perform with regard to patient access to specialist care, and the efficacy of interventions aimed at improving this.Methods and analysisSystematic review of the literature will be carried out on publications retrieved by searching the following electronic literature databases: EBSCOhost Megafile Ultimate (Cumulative Index to Nursing and Allied Health Literature (CINAHL), Academic Search Ultimate, APA Psychological Abstracts (PsycINFO), HealthSource), PubMed (Medical Literature Analysis and Retrieval System Online (MEDLINE)), Elsevier Bibliographic Database (Scopus), Excerpta Medica Database (EMBASE), Web of Science and The Cochrane Library. Articles published over a 10-year period (2012–2022) will be analysed to determine; current accessibility and availability problems faced by primary care services when referring patients to specialist care, and the effectiveness of interventions to improve primary care access to specialist services. Grey literature publications (ie, government reports, policy statements and issues papers, conference proceedings) will not be analysed in this review. Articles not published in English, Spanish or Portuguese will not be included. Two independent reviewers will conduct the initial screening, disagreements will be resolved by a third independent reviewer, following which data extraction and selection of eligible sources will be carried out. Selected articles will be categorised on study design, setting and participants. Methodological quality and heterogeneity will subsequently be assessed using the Mixed Methods Appraisal Tool. A descriptive approach will be used to review and synthesise the findings.Ethics and disseminationThis study does not require ethics committee review as it solely focuses on analysing published literature. Findings will be published and disseminated through a peer-reviewed journal.PROSPERO registration numberCRD42022354890.
Comparative study on risk prediction model of type 2 diabetes based on machine learning theory: a cross-sectional study
Authors: Wang Shu , Chen Rong , Wang Shuang , Kong Danli , Cao Rudai , Lin Chunwen , Luo Ling , Huang Jialu , Zhang Qiaoli , Yu Haibing , Ding Yuan Lin 
Published: August 2023

ObjectivesTo compare the prediction effects of six models based on machine learning theories, which can provide a methodological reference for predicting the risk of type 2 diabetes mellitus (T2DM).Setting and participantsThis study was based on the monitoring data of chronic disease risk factors in Dongguan residents from 2016 to 2018. The multistage cluster random sampling method was adopted at each monitoring site, and 4157 people were finally selected. In the initial population, we excluded individuals with more than 20% missing data and eventually included 4106 subjects.DesignK nearest neighbour algorithm and synthetic minority oversampling technique were used to process the data. Single factor analysis was used for preliminary selection of variables. The 10-fold cross-validation was used to optimise the parameters of some models. The accuracy, precision, recall and area under receiver operating characteristic curve (AUC) were used to evaluate the prediction effect of models, and Delong test was used to analyse the differences of AUC values of each model.ResultsAfter balancing data, the sample size increased to 8013, of which 4023 are patients with T2DM and 3990 in control group. The comparison results of the six models showed that back propagation neural network model has the best prediction effect with 93.7% accuracy, 94.6% accuracy, 92.8% recall and the AUC value of 0.977, followed by logistic model, support vector machine model, CART decision tree model and C4.5 decision tree model. Deep neural network has the worst prediction performance, with 84.5% accuracy, 86.1% precision, 82.9% recall and the AUC value of 0.845.ConclusionsIn this study, six types of risk prediction models for T2DM were constructed, and the predictive effects of these models were compared based on various indicators. The results showed that back propagation neural network based on the selected data set had the best prediction effect.