bluuebunny/crossref_metadata_2025_split huggingface dataset:
Bioremediation of Acetaminophen and Hydroxychloroquine by Kosakonia cowanii JCM 10956(T) with ecotoxicity studies
Authors: WAGHMODE MEGHMALA , PATIL NEHA , Gaikwad Dattatray 
Published: May 2023

Abstract

Acetaminophen and hydroxychloroquine are widely used drugs during COVID situations. Residual concentrations of acetaminophen and hydroxychloroquine have been detected in pharmaceutical industry wastewater, effluent treatment plants, and surface water. The present study was carried out on the bioremediation of acetaminophen (paracetamol) and hydroxychloroquine by using the bacterial isolate Kosakonia cowanii JCM 10956(T) (GenBank: OQ733302.1). Identification of the isolate was done using the 16S rRNA sequencing technique. The LC50 values for bacteria were determined for acetaminophen and hydroxychloroquine as 2186.70 and 1735.13 ppm, respectively. Isolate was found to degrade acetaminophen (1500 ppm) into hydroquinone after five days of incubation with an 81% biodegradation rate. Hydroxychloroquine (1000 ppm) was found to be degraded into oxalic acid with 7-chloroquinoline-4-amine and 4-aminoquinoline-7-ol as intermediates. After 15 days of incubation, 60% of hydroxychloroquine was found to be degraded. Acetaminophen and hydroxychloroquine biodegradation followed a first-order kinetic model with a rate constant of 0.339 d− 1 and 0.0618 d− 1, respectively. Half-lives for acetaminophen and hydroxychloroquine were found to be 2.05 and 11.2 days, respectively. Based on the analytical techniques of UV-visible spectra, HPLC, mass spectra, and proton nuclear magnetic spectroscopy (1H NMR) studies, biodegradative metabolites were identified. Ecotoxicological testing of the parent drug and degradative product was done using algal inhibition and shrimp lethality assays. The biodegradative product of acetaminophen, hydroquinone, has more algal toxicity and less toxicity against shrimp as compared to the parent drug. Whereas for the hydroxychloroquine biodegradative product, oxalic acid has less algal toxicity and more toxicity against shrimp compared to the parent drug. Industrial applications of hydroquinone and the metal leaching role of oxalic acid will give new insight into the bioconversion of expired paracetamol and hydroxychloroquine into value-added products.
Determinants of economic growth – A cross-country analysis
Authors: PUJARI SUDHARSANA REDDY , RAJASEKHAR MAMILLA 
Published: May 2023

Abstract
The current study aims to uncover the important economic factors that affect how quickly countries with various income levels grow. To examine the causal and cointegration relationship, the following variables are taken: Gross Domestic Product (GDP), Gross Domestic Savings (GDS), Foreign Direct Investment (FDI), Government External Debt (ED), Personal Remittances (PR), Government Final Consumption Expenditure (GFCE), Private Final Consumption Expenditure (PFCE), Net Official Development Aid (NODA), and Consumer Price Index (CPI). The results are obtained using the ARDL Bounds Test, Engle-Granger Causality Tests, and Error Correction Models (ECM). The findings provide substantial evidence in favor of the traditional theories of economic growth, which contend that domestic savings account for a large portion of an economy's growth rate. Results from all economic classes show that saving propels the economy except in the least-developed countries, where external debt drives the economies.
Assessing water quality in the Dong Nai River (Vietnam): implications for sustainable management and pollution control
Authors: Thi Thu Huong Tran , Quan Tran Anh , Hanh Nguyen Thi Hong , Tong Nguyen Xuan 
Published: May 2023

Abstract

Dong Nai River provides essential water resources for millions of people across 11 provinces and cities in Vietnam. However, the different pollution sources such as household, farming, and industrial operations have caused the river water quality to deteriorate over the past decade. To gain a comprehensive understanding of the river's surface water quality, this study employed the water quality index (WQI) across 12 different sampling sites. In total,144 water samples with 11 parameters were analyzed in accordance with the Vietnamese standard 08:2015/MONRE. Results revealed a range of surface water quality, from poor to good according to the VN-WQI (Vietnamese standard), and a medium even bad level in some months according to the NS-WQI (American standard). The study also identified temperature, coliform, and dissolved oxygen (DO) as strong contributors to WQI values (VN_WQI standard). Principal component analysis/factor analysis was used to determine pollution sources, with the results highlighting agricultural and domestic activities as the main contributors to river pollution. In conclusion, this study underscores the importance of effective planning and management of infrastructure zoning and local activities to improve the river's surface water quality and surrounding areas, as well as safeguard the well-being of the millions who depend on it.
CircPSMC3 Inhibits Colorectal Cancer Cell Growth and Migration by
Controlling miR-31-5p/YAP/β-Catenin
Authors: Wu Qi , Jin Ye , Yu Lingli , Zhang Bin , Chen Yun , Zhan Xiaobo , Liu Changfeng , Chen Zhi 
Published: May 2023

Introduction:
Colorectal cancer (CRC) is hackneyed cancer and a major lethiferous cancer.
Circular RNAs (CircRNAs) have been discovered to own important roles in controlling CRC
progression. CircPSMC3 is known to exhibit lower expression in diversified cancers. However, the
regulatory function of CircPSMC3 in CRC keeps unclear.

Methods:
The expression of CircPSMC3 and miR-31-5p was confirmed through RT-qPCR. The cell
proliferation was measured through CCK-8 and EdU assays. The protein expression of genes was
examined through a western blot. The cell invasion and migration were tested through Transwell and
wound healing assays. The binding ability between CircPSMC3 and miR-31-5p was confirmed
through the luciferase reporter assay.

Results:
CircPSMC3 exhibited lower expression in CRC tissues and cell lines. Additionally,
CircPSMC3 was revealed to suppress cell proliferation in CRC. Moreover, through Transwell and
wound healing assays, CircPSMC3 was discovered to repress CRC cell invasion and migration. In
CRC tissues, miR-31-5p expression was up-regulated and negatively correlated with CircPSMC3
expression. Further mechanism exploration experiments disclosed that CircPSMC3 is bound with
miR-31-5p to modulate the YAP/β-catenin axis in CRC. At last, through rescue assays, CircPSMC3
inhibited cell proliferation, invasion and migration through sponging miR-31-5p in CRC.

Conclusion:
Our work was the first time to probe the potential regulatory effects of CircPSMC3 in
CRC, and these above results uncovered that CircPSMC3 inhibited CRC cell growth and migration
through regulating miR-31-5p/YAP/β-catenin. This discovery hinted that CircPSMC3 may serve as a
useful therapeutic candidate for CRC.
Bioinformatics-aided Protein Sequence Analysis and Engineering
Authors: Zhang Wei , Wang Tianwen 
Published: May 2023

Abstract:
Most of the currently available knowledge about protein structure and function has been
obtained from laboratory experiments. As a complement to this classical knowledge discovery activity,
bioinformatics-assisted sequence analysis, which relies primarily on biological data manipulation,
is becoming an indispensable option for the modern discovery of new knowledge, especially when
large amounts of protein-encoding sequences can be easily identified from the annotation of highthroughput
genomic data. Here, we review the advances in bioinformatics-assisted protein sequence
analysis to highlight how bioinformatics analysis will aid in understanding protein structure and function.
We first discuss the analyses with individual protein sequences as input, from which some basic
parameters of proteins (e.g., amino acid composition, MW and PTM) can be predicted. In addition to
these basic parameters that can be directly predicted by analyzing a protein sequence alone, many
predictions are based on principles drawn from knowledge of many well-studied proteins, with multiple
sequence comparisons as input. Identification of conserved sites by comparing multiple homologous
sequences, prediction of the folding, structure or function of uncharacterized proteins, construction
of phylogenies of related sequences, analysis of the contribution of conserved related sites to protein
function by SCA or DCA, elucidation of the significance of codon usage, and extraction of functional
units from protein sequences and coding spaces belong to this category. We then discuss the
revolutionary invention of the "QTY code" that can be applied to convert membrane proteins into water-
soluble proteins but at the cost of marginal introduced structural and functional changes. As machine
learning has been done in other scientific fields, machine learning has profoundly impacted protein
sequence analysis. In summary, we have highlighted the relevance of the bioinformatics-assisted
analysis for protein research as a valuable guide for laboratory experiments.
Diversely Functionalized Pyridine Ring-fused Heterocycles and their Anticancer Properties
Authors: Narasimhamurthy Kereyagalahally H. , Girish Yarabahally R. , Swaroop Toreshettahally R. , Rangappa Kanchugarakoppal S. 
Published: May 2023

:
Among N-containing heterocycles, pyridine occupies a prominent position due to its presence
in nature. Many enzymes in living systems, which are involved in redox reactions, contain pyridine moiety.
In addition, its importance in medicinal chemistry and its presence in drugs are well documented. Several
pyridine containing compounds are well-known as tubulin polymerization inhibitors and are found to
bind with androgen receptors, kinases, carbonic anhydrase and topoisomerase. In recent years, researching
have been modifying pyridine containing entities to treat cancer. This review sheds light on recent
developments in anticancer studies of pyridine ring-fused heterocyclic compounds.
Depletion of DAND5 Hinders EMT in Mouse Embryonic Stem Cell
Differentiation
Authors: von Gilsa Lopes João , Inácio José M. , Marques Sara , Añez Sabrina B. , Belo José A. 
Published: May 2023

Background:
Dand5 encodes a protein that acts as an antagonist to Nodal/TGF-β and Wnt pathways. A mouse knockout (KO) model has shown that this molecule is associated with left-right asymmetry and cardiac development, with its depletion causing heterotaxia and cardiac hyperplasia.

Objective:
This study aimed to investigate the molecular mechanisms affected by the depletion of Dand5.

Methods:
DAND5-KO and wild-type embryoid bodies (EBs) were used to assess genetic expression with RNA sequencing. To complement the expression results that pointed towards differences in epithelial to mesenchymal transition (EMT), we evaluated migration and cell attachment. Lastly, in vivo valve development was investigated, as it was an established model of EMT.

Results:
DAND5-KO EBs progress faster through differentiation. The differences in expression will lead to differences in the expression of genes involved with Notch and Wnt signalling pathways, as well as changes in the expression of genes encoding membrane proteins. Such changes were accompanied by lower migratory rates in DAND5-KO EBs, as well as higher concentrations of focal adhesions. Within valve development, Dand5 is expressed in the myocardium underlying future valve sites, and its depletion compromises correct valve structure.

Conclusion:
The DAND5 range of action goes beyond early development. Its absence leads to significantly different expression patterns in vitro and defects in EMT and migration. These results have an in vivo translation in mouse heart valve development. Knowledge regarding the influence of DAND5 in EMT and cell transformation allows further understanding of its role in development, or even in some disease contexts, such as congenital heart defects.
Electrospinning Fabrication of PA66 Colloidal Crystal Fibers with Various
Morphologies
Authors: Guangchen Pei , Pingping Wu , Jingxia Wang 
Published: May 2023

Abstract:
Colloidal crystal (CC) fiber has unique light manipulation properties, fiber flexibility,
and the potential to be used in the textile industry as an alternative to chemical dyes. Introducing
polyamide 66 (PA66) into CC fiber can effectively improve the performance of fibers. In this
study, polyamide 66 (PA66) CC fibers with various morphologies were fabricated by electrospinning
using high-tensile PA66 and P(St-MMA-AA) latex particles as raw materials, such as closepacked,
inlaid, noodle-like, spindle knots, bamboo-like, semi-enclosed, hat-like, etc. The formation
mechanism of various fibers was analyzed based on the phase separation and assembly
interaction. The prepared PA66 CC fiber film was reported to have unique structural color and
enhanced mechanical properties, which can be used as a substrate for drawing various patterns.
This work will provide a novel idea for the fabrication of functional CC fiber, which is helpful for
the potential applications in the textile industry.
Ionic Liquid Supported Organocatalysts for the Asymmetric Mannich-
Type Reaction of α-cyano α-sulfonyl Carbanions
Authors: Srivastava Vivek 
Published: May 2023

Introduction:
We synthesized proline functionalized ionic liquid and further character-ized it with analytical techniques.

Method:
After getting proper structural information on the above ionic liquid, we utilized them as a catalyst for the asymmetric Mannich-Type Reaction.

Result:
We synthesized 6 different types of β-amino α-cyano sulfones derivatives as Mannich ad-duct using various combinations of different imines and aryl sulphonyl as pre-nucleophiles.

Conclusion:
We obtained the corresponding chiral Mannich adduct followed by simple ether washing in excellent yield and stereoselectivity. We recycled the catalyst up to 6 runs without los-ing the catalytic activity.
Assessment of Patients' Referral Patterns with Complaints of
Self-harm and Aggression in the COVID-19 Era
Authors: Houjaghan Amirmasoud Kazemzadeh , Arya Pantea , Aarabi Sepideh , Ashraf Haleh , Bahreini Maryam 
Published: May 2023

Background:
Due to the high transmission rate of COVID-19, the high prevalence
of the disease, the high mortality rate, and its effects on mental health, we aimed to assess the
current status of psychiatric symptoms.

Methods:
In this observational study, we have assessed various psychiatric presentations and
disorders before and after the COVID-19 pandemic within the same time limit. Data have been
obtained from the psychiatric interview performed by an attending physician in psychiatry.

Results:
The following features have been observed after the pandemic: increased depressed
mood, irritability, crime trend, physical violations, personality disorders along with improved
family support, and decreased suicidal ideation. No significant difference has been observed in
the rate of response to psychotherapy and psychiatric medications before and after the time of
the pandemic.

Conclusion:
Increased physical threat and aggression, substance use, and symptoms of
psychosis were more frequently observed in the time of the pandemic. The physical threat was
mainly committed by younger patients with psychiatric illnesses.
Application of CBCT Technology in Forensic Odontology: A Narrative
Review
Authors: Hassanaly Moustansir , Morais Caldas Inês , Teixeira Alexandra , Pérez-Mongiovi Daniel 
Published: May 2023

Background:
Cone-Beam Computer Tomography (CBCT) is a non-invasive, rapid, cost-effective, and low-radiation technique. Being a three-dimensional (3D) imaging process, it is better suited for the analysis of 3D structures like teeth, bones, or facial sinuses. CBCT is already widely used in dentistry and its application in forensic odontology is promising.

Objective:
This study aimed to provide an overview of CBCT use in forensic dentistry.

Method:
A bibliographic search using PUBMED was performed with the following keyword combinations: ("Cone-Beam Computed Tomography" [MeSH Terms]) OR (cbct)) AND (forensic dentistry) OR ("Forensic Dentistry" [Mesh]) and on the Scopus platform using the keywords “cbct forensic dentistry”. Considering the inclusion and exclusion criteria, the final selection resulted in 68 studies.

Results:
Articles subjects were as follows: 11 studies (16%) on comparative identification, 4 (5%) on identification by bitemarks, 30 (44%) on age estimation, 20 (29%) on sex estimation, and 7 (10%) on facial reconstruction. CBCT technology proved to be an accurate tool for age estimation (particularly in the pulp narrowing technique), sex estimation, bitemarks analysis, and facial recon-struction.

Conclusion:
CBCT's increasing use in dental clinics makes a huge quantity of data available. Pro-fessionals should examine how to organize and disseminate these valuable antemortem data. Train-ing is mandatory to understand CBCT’s technical limitations as well as manage the presence of artifacts. Further studies should be made on larger samples to fully understand the potential of CBCT technology in forensics.
Culture notes in ‘Bahasaku 1: Ayo bicara bahasa Indonesia’
Authors: Sahasti Julananda Putri 
Published: May 2023

Language and culture are two things that are closely related to each other. The purpose of this study is to describe the cultural notes presented in the book "Bahasaku 1: Ayo Bicara Bahasa Indonesia". The research method used in this study is descriptive qualitative with a content analysis approach. The data source in this study was the book 'Bahasaku 1: Ayo Bicara Bahasa Indonesia' compiled by the Indonesian Language Institute Kursul Alam, Yogyakarta. Data were analyzed in five stages, namely compiling notes, disassembling data, rearranging based on data, interpreting, and drawing conclusions. Based on the results of the data analyses, it can be concluded that the cultural notes in the book My Language 1: Let's Talk Indonesian can be grouped into eight categories, namely: 1) greetings; 2) eating habits; 3) rubber watch; 4) small talks; 5) mudik; 6) coutercies of guests and hosts; 7) beach suits and the myth of Nyai Roro Kidul; and 8) 'kiri' in the context of public transportation. The cultural notes in the book "Bahasaku 1: Ayo Bicara Bahasa Indonesia" belonging to Alam Bahasa Indonesia have been well organized. The cultural notes in this textbook are not only specifically listed in the "Cultural Notes" section at the end of each lesson, but, they are also integrated well into every text in the lesson. My language book 1 contains clear concepts and understandings that can accurately describe the cultures that have developed in the Indonesian society.Keywords: culture notes, textbook, foreign language, cultural understanding, BIPA
Pre-service teachers’ voices of international teaching practicum in Indonesian elementary school
Authors: Setyaningsih Ani , Diyanti B. Yuniar , Nurhayati Lusi 
Published: May 2023

International teaching practicums help pre-service teachers (PSTs) advance professionally in teaching and gain multicultural competence. This study aims at describing an investigation of challenges and problems faced by the pre-service teachers during their international teaching program in an Indonesian elementary school and how to cope with them revealed by the PSTs' voices. This qualitative case study started with conducting a survey questionnaire covering various aspects of results, challenges, and problems regarding the aspects of pedagogical, content knowledge, technology, and cultural gaps faced by 7 international pre-service teachers (PSTs) from the Netherlands. The survey results were then further examined through in-depth interviews with the PSTs and thematic analysis was conducted to examine the data. The findings showed that there were three categories of problems faced by the PSTs: issues from the students, problems from the PSTs, and problems from the schools or stakeholders. The PSTs could adapt to the situation and find solutions to the problems. They tried to be "Indonesians," enjoyed every activity and difference, and communicated to the right person to solve the problems. The study also revealed that the PSTs hoped that the lecturers could have spent more time at school and given them more advice. They wished the university could improve the scheduling system and give them a chance to teach in more than one school to be able to experience different school cultures. This study recommends further investigation of how a good international teaching program should be developed and managed.Keywords: pre-service teachers, international teaching practicum, teaching English to young learners
The translation of conceptual metaphor in political news
Authors: Siriam Siriam , Widyastuti Susana 
Published: May 2023

Metaphorical expressions (MEs) are abundant in political text and their translation ‎poses challenges particularly for retaining the implicit meaning. This research focuses on examining the use of conceptual metaphors (CMs) ‎in Indonesian ‎political news and their English translation. The objectives ‎ are to identify types and the source domain of CMs and to explain how the CMs are translated.‎ ‎Grounding on mixed-methods, the data were metaphorical expressions in ‎ the Indonesian and English versions of Tempo weekly magazines in April, May, and June in 2021. Metaphor Identification Procedure (MIP) was ‎applied to determine the metaphoricity of metaphors in the STs and Indonesian dictionary (KBBI) was also employed to avoid intuition ‎decision-making. ‎The results indicate that CMs are commonly used in ‎political news in order to popularize, concretize, and dramatize issues. Structural metaphors are found ‎as the most dominant type and twenty source domains are found ‎in the STs with war metaphor as the most significant domain. In terms of the translation, the study demonstrates that non ‎metaphorical translation is the most frequently used technique to render metaphors ‎into English TT. As a result, the translation reduces the ‎metaphoric function of the original expressions.Keywords: political news, conceptual metaphor, translation technique