bluuebunny/crossref_metadata_2025_split huggingface dataset:
Efficiency of agricultural subsidies in Kazakhstan
Authors: Seitov S. K. 
Published: May 2023

Relevance. The efficiency of budget expenditures can be considered on the basis of achievement of a socially significant planned result. We have a question which result should be recognized as the target one. The efficiency of subsidies is embodied in the efficiency of the activity of producers in agriculture. In turn, the efficiency of producers' activity is expressed through factor productivity. The issue of correlation of subsidies with the dynamics of factor productivity needs more detailed elaboration, which determines the relevance of the work. The object of the research is the agricultural subsidy system. The subject of the research is the economic efficiency of agricultural subsidies in Kazakhstan.Methods. The author applies economic and statistical analysis to study the time series on the economic efficiency of subsidies in Kazakhstan. The survey uses comparative analysis to compare the economic efficiency of subsidizing agriculture in Kazakhstan and Russia. System analysis serves to determine the factors influencing the dynamics of the main indicators of agricultural development in Kazakhstan and Russia.Results. In order to assess the economic efficiency of agricultural subsidies, the study proposes to correlate subsidies with the dynamics of output, productivity of individual factors. The work reveals that subsidies do not create the conditions under which many agricultural producers would consciously move to innovative and environmentally friendly technologies.
Estimation of acute toxicity of bioinsecticide Turinbash-G for honey bee <i>Apis mellifera mellifera L.</i>
Authors: Mishukovskaya G. S. , Shelekhov D. V. , Giniyatullin M. G. , Andreeva A. V. 
Published: May 2023

Introduction. Pesticide poisoning is one of the main causes of mass death of bee colonies observed in different countries of the world. However, at present, abandoning the use of plant protection products is not possible, since insects and crop weeds are responsible for the loss of at least half of the world's food supply. An increase in insect pest activity due to global warming will lead to even greater losses. All this dictates the need for a wider introduction of biological methods of plant protection. Bioinsecticides based on entomopathogens can become an alternative to chemicals that have a pronounced toxic effect on nontarget organisms.Methods. The object of the study was honey bee Apis mellifera mellifera L. of the Carpathian breed, subjected to oral and contact exposure to Turinbash-G, the active ingredient of which is the bacterium Bacillus thuringiensis subsp. aizawai strain 12K and Bacillus thuringiensis subsp. thuringiensis, strain VNIIVEA-177. As a guide, the work used «Methodological recommendations for assessing the effect and potential hazard of pesticides for honeybees», approved by the Russian Academy of Agricultural Sciences and the Department of Veterinary Medicine of the Ministry of Agriculture of the Russian Federation, as well as GOST 33038-2014 and GOST 33039-2014 «Mеthods for testing chemical products posing a danger to the environment.» In the course of the studies, the acute oral toxicity and acute contact toxicity of the active substance of Turinbash-G.Results. Studies have not revealed signs of acute toxicity of the Turinbash-Zh bioinsecticide for honey bees both with oral and with contact exposure to the drug. The maximum mortality rate of bees in the experimental groups 96 hours after the end of exposure to the studied drug is 16.7%. LD50 in determining the acute oral toxicity of the active substance of the drug Turinbash-Zh — above the value of 1·106 CFU/bee, LK50 — above 100 ml/l; in determining the acute contact toxicity LD50 above the value of 1·105 CFU/bee, LK50 — above 100 ml/l.
Evaluation of meat qualities of crossbred young pigs of different breeding
Authors: Belookov A. A. , Belookova O. V. , Stvolov S. S. , Gritsenko S. A. , Rebezov M. B. , Zyablitseva M. A. 
Published: May 2023

Relevance.To ensure the profitability of production, it is necessary to use certain schemes for crossing pigs of different breeds. Since the choice of the correct crossing scheme can significantly affect the final result. Crossbred animals, due to the effect of heterosis, are superior in productive qualities to the original maternal and paternal breeds. The paper presents an assessment of the meat qualities of local young pigs obtained as a result of industrial three-breed crossing.Methods.To implement the scientific and economic experience, three groups of sows of pairs-analogues of a large white breed from the company Hypor (KB Hypor) were formed. Sows of the 1st group were crossed with boars of the Landrace breed from PIC (Landrace Pic), the 2nd — with boars of the Landrace breed from Genesus Genetics (Landrace Genesus), the 3rd — with boars of the Landrace breed from Hypor (Landrace Hypor), as a result, two-breed crossbreeds (F1) were obtained. Further, the obtained crossbred sows (F1) of the 1st, 2nd and 3rd experimental groups were crossed with boars of the Duroc breed from Genesus Genetics, as a result, they received commercial young (F2).Results.The highest pre-slaughter live weight was obtained from animals of the 1st group (125.12 kg). This is more than in the 2nd and 3rd experimental groups, respectively, by 5.8% and 4.0%. Significantly, the highest slaughter weight was in the 1st group (93.51 kg), and the lowest — in the 2nd (87.8 kg). Most of the meat was obtained from animals of the 1st group — 62.85 kg (p ≤0.05). This is higher than in the 2nd and 3rd experimental groups, respectively, by 8.2% and 5.9%.
Influence different form of selenium to production and metatabolic process of young cattle
Authors: Klementiev M. I. , Chabaev M. G. , Tsis E. Yu. , Nekrasov R. V. 
Published: May 2023

Relevance. In modern conditions of intensive animal husbandry, primary importance is attached to the feeding of chelated forms of trace elements, in particular selenium. The purpose of the research was to develop norms for feeding organic selenium for calves in the dairy and post-dairy growing periods.Materials and methods. Scientific, economic and balance experience in JSC «Molodi» of the Chekhov district of the Moscow region on 55 heads of young cattle of a black-and-white Holstein breed. During the studies, the calves of the 1st control group (C-) were fed the feed of the main diet (OR) without any Se additives, while the calves of the 2nd experimental group (C+) were fed OR and Se in an amount of 0.30 mg/kg of dry matter (CB) of the diet (additionally, a premix was set, containing sodium selenite), animals of the 3rd experimental group (E100) were fed in addition to OR Se in organic form plus 0.30 mg/kg of the ration (in the dairy and post-dairy periods, analogues from the 4th experimental group (E75) were fed OR plus 0.22 mg/kg of the Se ration (in organic form), calves of the 5th experimental group (E50) were fed as a part of OR 0.15 mg/kg Se (in organic form).Results. On average, over the period of the experiment, the total average daily increase in live weight of calves of the experimental groups was, respectively, 719 g, 736 g, 781 g, 710 g (or by 6,8%, 9,4%, 16%, 5,5%) and by 8.6% more compared to the control and calves of the 2nd an experimental group that received an inorganic form of selenium according to the norms of the needs of dairy cattle.
Condominium Townhouse Investment
Authors: Wakil Gulraze , Petruska Karin 
Published: May 2023

<p>This accounting case involves the evaluation of various alternatives based on a real-life personal property investment. The Condominium Townhouse Investment (CTI) case uses introductory management accounting concepts and analyses to introduce students to the case method of learning. The main concepts reinforced are relevant costs and revenues, including diﬀerentiating between opportunity and sunk costs. Students are not only asked to deﬁne and identify these items, but to consider choices centered on the decision alternatives in their role as accountants in public practice. This case is especially valuable because it oﬀers students the chance to become engaged in an analysis and decision-making situation that they can relate to both personally and professionally because, one day, they themselves are likely to become involved in a home ownership decision. The case focuses on real estate as a personal business investment, and requires both qualitative and quantitative analysis, with more emphasis on the qualitative aspect. In addition, the teaching notes include methods and strategies for students to use in an introductory managerial accounting class or level-1 MBA class. </p>
Seeking Fulfillment: Comparing Role-Play in Table-top Gaming and World of Warcraft
Authors: Pittman Jason , Paul Christopher 
Published: May 2023

In the midst of the massive growth in the numbers of people participating in massively multiplayer online role-playing games (MMORPGs), study of the role-playing within these games is increasingly marginalized, as role-playing servers are developed to segregate player bases and as scholars increasingly focus on the non-role-playing aspects of online game play. We seek toaddress that disconnection by studying relative levels of fulfillment and frequency of tabletop and online role-playing for World of Warcraft (WoW) subscribers.
Finally, demographic information taken from the players themselves aids in guiding future research about which type of role-play gamers are more likely to participate in and find fulfilling. By looking at the age, location, and gender of players the relative merits of each type of role-playing is discussed, pointing to strategies to capitalize on the strengths and minimize the failings of each venue for role-playing. This study aids both the online and offline gaming industry in better understanding their target role-playing audiences, while providing a grounded study of avid role-players to support analysis of game dynamics.
Wujud Aspek Psikologis dalam Sajak Penyair Jambi Ari Setya Ardhi
Authors: Amral Sainil , Sumiharti Sumiharti 
Published: May 2023

The purpose of this study is to describe the form of conveying the psychological aspects of the psychiatric form contained in the poems of the Jambi poet Ari Setya Ardhi. Jambi poet means a poet who lives in the Jambi area, actively works, and always documents his rhymes in individual anthologies and joint anthologies. This research includes descriptive research with a content analysis approach to documents in the form of literary works. The data in this study are rhyme texts that refer to the psychological aspects of the psychiatric form of depression and the psychiatric form of anxiety. The results showed that in the poems of the Jambi poet Ari Setya Ardhi depression was identified through the use of sounds, word choices, complete text, typography, and the use of imagery
Titanium surface interacting with blood clot enhanced migration and osteogenic differentiation of bone marrow mesenchymal stem cells
Authors: Li Jia , Zhao Juan , Xu Yangbo , Xu Antian , He Fuming 
Published: May 2023

Introduction: Blood clot formation is the initial phase upon implantation, and the feature of blood clot orchestrates the following complement system activation, coagulation cascade, and bone marrow mesenchymal stromal cells (BMSCs) recruitment. This study aimed to investigate the effect of implant surface on blood-material interactions and subsequent BMSC cellular behaviors.Methods: This study was established to imitate the physiological process of implantation in vivo and in vitro. Whole blood was incubated with polished titanium (PT) surfaces and sandblasted and double acid-etching (SLA) surfaces for 10 min or 2 h, then seeded with BMSCs. The adhesion, proliferation, migration, and differentiation of cells were studied at specific time points. Titanium implants were implanted into the tibia in vivo and were screwed out after implantation. The activation of the coagulation cascade, platelets, complement system, and clot networks were assessed and further quantitatively analyzed.Results: Compared with the PT surface, the SLA surface induced the earlier and stronger blood coagulation cascade and formed a more stratified clots network with fibrinogen, platelets, and CD14 positive cell. The adhesion, proliferation, and migration of BMSCs were enhanced by pre-incubated surfaces. The higher levels of the osteogenic-related genes, ALP activity, and calcium nodule formation were showed on SLA surfaces with blood incubation.Conclusion: SLA titanium surfaces play a role in influencing the formation of blood clots and coordinating surface-blood interactions and cell biological processes. These findings provide the idea of modifying the blood clots formed on the implant surface by biomaterials modification and thus has implications for the development of better osteogenic biomaterials.
Efficient adeno-associated virus serotype 5 capture with affinity functionalized nanofiber adsorbents
Authors: Neto Salomé , Mendes João P. , Santos Susana B. Dos , Solbrand Anita , Carrondo Manuel J. T. , Peixoto Cristina , Silva Ricardo J. S. 
Published: May 2023

Adeno-associated viruses (AAVs) are one of the most promising tools for gene therapy applications. These vectors are purified using affinity and ion exchange chromatography, typically using packed beds of resin adsorbents. This leads to diffusion and pressure drop limitations that affect process productivity. Due to their high surface area and porosity, electrospun nanofiber adsorbents offer mass transfer and flow rate advantages over conventional chromatographic media. The present work investigated the use of affinity cellulose-based nanofiber adsorbents for adeno-associated virus serotype 5 (AAV5) capture, evaluating dynamic binding capacity, pressure drop, and AAV5 recovery at residence times (RT) less than 5 s. The dynamic binding capacity was found to be residence time-dependent, but nevertheless higher than 1.0 × 1014 TP mL−1 (RT = 1.6 s), with a pressure drop variation of 0.14 MPa obtained after loading more than 2,000 column volumes of clarified AAV5 feedstock. The single affinity chromatography purification step using these new affinity adsorbents resulted in 80% virus recovery, with the removal of impurities comparable to that of bead-based affinity adsorbents. The high binding capacity, virus recovery and reduced pressure drop observed at residence times in the sub-minute range can potentially eliminate the need for prior concentration steps, thereby reducing the overall number of unit operations, process time and costs.
Microenvironment commits breast tumor ECs to dedifferentiation by micro-RNA-200-b-3p regulation and extracellular matrix remodeling
Authors: Wilkus-Adamczyk Kinga , Brodaczewska Klaudia , Majewska Aleksandra , Kieda Claudine 
Published: May 2023

Introduction: Hypoxia shapes the tumor microenvironment, modulates distinct cell population activities, and activates pathological angiogenesis in cancer, where endothelial cells (ECs) are the most important players. This study aimed to evidence the influences of the tumor microenvironment on the global gene expression pattern characteristic for ECs and the distinct responses displayed by tumor-derived ECs in comparison to the healthy endothelium during endothelial to mesenchymal transition (EndMT) and its regulation by miR-200-b-3p.Methodology: Immortalized lines of ECs from the same patient with breast cancer, healthy breast tissue (HBH.MEC), and primary tumor (HBCa.MEC) were used. The experiments were performed in normoxia and hypoxia for 48 h. By using the wound healing test, we investigated the migration abilities of ECs. Global gene expression analysis with NGS was carried out to detect new pathways altered in pathological ECs and find the most changed miRNAs. The validation of NGS data from RNA and miRNA was estimated by qPCRs. Mimic miR-200b-3p was used in HBH.MEC, and the targets VEGF, Bcl2, ROCK2, and SP1 were checked.Results: Hypoxia influences EC migration properties in wound healing assays. In hypoxia, healthy ECs migrate slower than they do in normoxia, as opposed to HBCa.MEC, where no decreased migration ability is induced by hypoxia due to EndMT features. NGS data identified this process to be altered in cancer ECs through extracellular matrix (ECM) organization. The deregulated genes, validated by qPCR, included SPP1, ITGB6, COL4A4, ADAMST2, LAMA1, GAS6, PECAM1, ELN, FBLN2, COL6A3, and COL9A3. NGS also identified collagens, laminins, fibronectins, and integrins, as being deregulated in tumor-derived ECs. Moreover, the analysis of the 10 most intensively modified miRNAs, when breast tumor–derived ECs were compared to healthy ECs, shed light on miR-200b-3p, which is strongly upregulated in HBCa.MECs when compared to HBH.MECs.Discussion and conclusion: The pathological ECs differed significantly, both phenotypically and functionally, from the normal corresponding tissue, thus influencing their microenvironment cross-talk. The gene expression profile confirms the EndMT phenotype of tumor-derived ECs and migratory properties acquisition. Moreover, it indicates the role of miR-200b-3p, that is, regulating EndMT in pathological ECs and silencing several angiogenic growth factors and their receptors by directly targeting their mRNA transcripts.
Complete genome analysis of Tequatrovirus ufvareg1, a Tequatrovirus species inhibiting Escherichia coli O157:H7
Authors: Lopez Maryoris Elisa Soto , Gontijo Marco Tulio Pardini , Cardoso Rodrigo Rezende , Batalha Laís Silva , Eller Monique Renon , Bazzolli Denise Mara Soares , Vidigal Pedro Marcus Pereira , Mendonça Regina Célia Santos 
Published: May 2023

IntroductionBacteriophages infecting human pathogens have been considered potential biocontrol agents, and studying their genetic content is essential to their safe use in the food industry. Tequatrovirus ufvareg1 is a bacteriophage named UFV-AREG1, isolated from cowshed wastewater and previously tested for its ability to inhibit Escherichia coli O157:H7.MethodsT. ufvareg1 was previously isolated using E. coli O157:H7 (ATCC 43895) as a bacterial host. The same strain was used for bacteriophage propagation and the one-step growth curve. The genome of the T. ufvareg1 was sequenced using 305 Illumina HiSeq, and the genome comparison was calculated by VIRIDIC and VIPTree.ResultsHere, we characterize its genome and compare it to other Tequatrovirus. T. ufvareg1 virions have an icosahedral head (114 x 86 nm) and a contracted tail (117 x 23 nm), with a latent period of 25 min, and an average burst size was 18 phage particles per infected E. coli cell. The genome of the bacteriophage T. ufvareg1 contains 268 coding DNA sequences (CDS) and ten tRNA genes distributed in both negative and positive strains. T. ufvareg1 genome also contains 40 promoters on its regulatory regions and two rho-independent terminators. T. ufvareg1 shares an average intergenomic similarity (VIRIDC) of 88.77% and an average genomic similarity score (VipTree) of 88.91% with eight four reference genomes for Tequatrovirus available in the NCBI RefSeq database. The pan-genomic analysis confirmed the high conservation of Tequatrovirus genomes. Among all CDS annotated in the T. ufvareg1 genome, there are 123 core genes, 38 softcore genes, 94 shell genes, and 13 cloud genes. None of 268 CDS was classified as being exclusive of T. ufvareg1.ConclusionThe results in this paper, combined with other previously published findings, indicate that T. ufvareg1 bacteriophage is a potential candidate for food protection against E. coli O157:H7 in foods.
Post-COVID-19 syndrome and diabetes mellitus: a propensity-matched analysis of the International HOPE-II COVID-19 Registry
Authors: Abumayyaleh Mohammad , Núñez Gil Iván J. , Viana-LLamas María C. , Raposeiras Roubin Sergio , Romero Rodolfo , Alfonso-Rodríguez Emilio , Uribarri Aitor , Feltes Gisela , Becerra-Muñoz Víctor Manuel , Santoro Francesco , Pepe Martino , Castro Mejía Alex Fernando , Signes-Costa Jaime , Gonzalez Adelina , Marín Francisco , López-País Javier , Manzone Edoardo , Vazquez Cancela Olalla , Paeres Carolina Espejo , Masjuan Alvaro López , Velicki Lazar , Weiß Christel , Chipayo David , Fernandez-Ortiz Antonio , El-Battrawy Ibrahim , Akin Ibrahim ,   HOPE COVID-19 investigators
Published: May 2023

BackgroundDiabetes mellitus (DM) is one of the most frequent comorbidities in patients suffering from severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) with a higher rate of severe course of coronavirus disease (COVID-19). However, data about post-COVID-19 syndrome (PCS) in patients with DM are limited.MethodsThis multicenter, propensity score-matched study compared long-term follow-up data about cardiovascular, neuropsychiatric, respiratory, gastrointestinal, and other symptoms in 8,719 patients with DM to those without DM. The 1:1 propensity score matching (PSM) according to age and sex resulted in 1,548 matched pairs.ResultsDiabetics and nondiabetics had a mean age of 72.6 ± 12.7 years old. At follow-up, cardiovascular symptoms such as dyspnea and increased resting heart rate occurred less in patients with DM (13.2% vs. 16.4%;p= 0.01) than those without DM (2.8% vs. 5.6%;p= 0.05), respectively. The incidence of newly diagnosed arterial hypertension was slightly lower in DM patients as compared to non-DM patients (0.5% vs. 1.6%;p= 0.18). Abnormal spirometry was observed more in patients with DM than those without DM (18.8% vs. 13;p= 0.24). Paranoia was diagnosed more frequently in patients with DM than in non-DM patients at follow-up time (4% vs. 1.2%;p= 0.009). The incidence of newly diagnosed renal insufficiency was higher in patients suffering from DM as compared to patients without DM (4.8% vs. 2.6%;p= 0.09). The rate of readmission was comparable in patients with and without DM (19.7% vs. 18.3%;p= 0.61). The reinfection rate with COVID-19 was comparable in both groups (2.9% in diabetics vs. 2.3% in nondiabetics;p= 0.55). Long-term mortality was higher in DM patients than in non-DM patients (33.9% vs. 29.1%;p= 0.005).ConclusionsThe mortality rate was higher in patients with DM type II as compared to those without DM. Readmission and reinfection rates with COVID-19 were comparable in both groups. The incidence of cardiovascular symptoms was higher in patients without DM.
LINC00426 is a potential immune phenotype-related biomarker and an overall survival predictor in PAM50 luminal B breast cancer
Authors: Fonseca-Montaño Marco Antonio , Cisneros-Villanueva Mireya , Coales Isabelle , Hidalgo-Miranda Alfredo 
Published: May 2023

Background: Breast cancer (BRCA) represents the most frequent diagnosed malignancy in women worldwide. Despite treatment advances, BRCAs eventually develop resistance to targeted therapies, resulting in poor prognosis. The identification of new biomarkers, like immune-related long non-coding RNAs (lncRNAs), could contribute to the clinical management of BRCA patients. In this report, we evaluated the LINC00426 expression in PAM50 BRCA subtypes from two clinical independent cohorts (BRCA-TCGA and GEO-GSE96058 datasets).Methods and results: Using Cox regression models and Kaplan-Meier survival analyses, we identified that LINC00426 expression was a consistent overall survival (OS) predictor in luminal B (LB) BRCA patients. Subsequently, differential gene expression and gene set enrichment analyses identified that LINC00426 expression was associated with different immune-related and cancer-related pathways and processes in LB BRCA. Additionally, the LINC00426 expression was correlated with the infiltration level of diverse immune cell populations, alongside immune checkpoint and cytolytic activity-related gene expression.Conclusion: This evidence suggests that LINC00426 is a potential biomarker of immune phenotype and an OS predictor in PAM50 LB BRCA.