bluuebunny/crossref_metadata_2025_split huggingface dataset:
3-Hydroxy-3-methylglutaryl coenzyme A reductase genes from Glycine max regulate plant growth and isoprenoid biosynthesis
Authors: Wang Shuai , Feng Yumei , Lou Yin , Niu Jingping , Yin Congcong , Zhao Jinzhong , Du Weijun , Yue Aiqin 
Published: March 2023

AbstractIsoprenoids, a large kind of plant natural products, are synthesized by the mevalonate (MVA) pathway in the cytoplasm and the 2-C-methyl-d-erythritol 4-phosphate (MEP) pathway in plastids. As one of the rate-limiting enzymes in the MVA pathway of soybean (Glycine max), 3-hydroxy-3-methylglutaryl coenzyme A reductase (HMGR) is encoded by eight isogenes (GmHMGR1–GmHMGR8). To begin, we used lovastatin (LOV), a specific inhibitor of GmHMGR, to investigate their role in soybean development. To further investigate, we overexpressed the GmHMGR4 and GmHMGR6 genes in Arabidopsis thaliana. The growth of soybean seedlings, especially the development of lateral roots, was inhibited after LOV treatment, accompanied by a decrease in sterols content and GmHMGR gene expression. After the overexpression of GmHMGR4 and GmHMGR6 in A. thaliana, the primary root length was higher than the wild type, and total sterol and squalene contents were significantly increased. In addition, we detected a significant increase in the product tocopherol from the MEP pathway. These results further support the fact that GmHMGR1–GmHMGR8 play a key role in soybean development and isoprenoid biosynthesis.
Land-use induced soil carbon stabilization at the expense of rock derived nutrients: insights from pristine Andean soils
Authors: Jungkunst Hermann F. , Heitkamp Felix , Doetterl Sebastian , Sylvester Steven P. , Sylvester Mitsy D. P. V. , Vetter Vanessa , Maqsood Shafique , Zeppenfeld Thorsten , Kessler Michael , Fiedler Sabine 
Published: March 2023

AbstractSoils contain significantly more carbon than the atmosphere, hence we should understand how best to stabilize it. Unfortunately, the role of human interventions on soil organic carbon (SOC) persistence in the Anthropocene remains vague, lacking adequate sites that allow unbiased direct comparisons of pristine and human influenced soils. Here we present data from a unique study system in the High Andes that guarantees pristineness of the reference sites by physical inaccessibility through vertical cliffs. By comparing the isotopic signatures of SOC, mineral related carbon stabilization, and soil nutrient status across grazed versus pristine soils, we provide counterintuitive evidence that thousands of years of pastoralism increased soil C persistence. Mineral associated organic carbon (MAOC) was significantly higher in pastures. Land use increased poorly crystalline minerals (PCM’s), of which aluminum correlated best with MAOC. On the other hand, human’s acceleration of weathering led to acidification and higher losses of cations. This highlights a dilemma of lower soil quality but higher persistence of SOC due to millennia of pastoralism. The dynamics of soil genesis in the Anthropocene needs better understanding, but if human-induced weathering proves generally to promote soil carbon persistence it will need to be included in climate—soil feedback projections.
On taylor correlation functions in isotropic turbulent flows
Authors: Chen Wei 
Published: March 2023

AbstractA turbulent flow can be characterized by Taylor correlation functions which are obtained empirically, understood by statistical mechanics and regarded as universal. Here, we show that Taylor correlations are analytically derived by hypothesizing turbulence as a phenomenon of superfluids at resonance. Leveraging from a recent study on heat transfer at the speed of sound, we derived and fitted the longitudinal and lateral turbulent velocities in an isotropic, turbulent flow. The concept of the boundary of the second law helps to specify the integration constants in the solution. From the velocity profiles, Taylor’s correlation functions are analytically determined. From the linearity of the eigenfunction, we introduce amplitude and frequency factors. These factors are curve-fitted with two experimental dataset. Additional experimental datasets in the public domain are compared to the correlations, which shows that the theory agrees with experiments very well in isotropic flows. The analytical correlation functions help to elucidate observations that experiments and statistical mechanics have challenges to explain.
Horses cross-modally recognize women and men
Authors: Gouyet Chloé , Ringhofer Monamie , Yamamoto Shinya , Jardat Plotine , Parias Céline , Reigner Fabrice , Calandreau Ludovic , Lansade Léa 
Published: March 2023

AbstractSeveral studies have shown that horses have the ability to cross-modally recognize humans by associating their voice with their physical appearance. However, it remains unclear whether horses are able to differentiate humans according to different criteria, such as the fact that they are women or men. Horses might recognize some human characteristics, such as sex, and use these characteristics to classify them into different categories. The aim of this study was to explore whether domesticated horses are able to cross-modally recognize women and men according to visual and auditory cues, using a preferential looking paradigm. We simultaneously presented two videos of women and men’s faces, while playing a recording of a human voice belonging to one of these two categories through a loudspeaker. The results showed that the horses looked significantly more towards the congruent video than towards the incongruent video, suggesting that they are able to associate women’s voices with women’s faces and men’s voices with men’s faces. Further investigation is necessary to determine the mechanism underlying this recognition, as it might be interesting to determine which characteristics horses use to categorize humans. These results suggest a novel perspective that could allow us to better understand how horses perceive humans.
Survival in non-small cell lung cancer patients with versus without prior cancer
Authors: Sato Akira , Morishima Toshitaka , Takeuchi Masato , Nakata Kayo , Kawakami Koji , Miyashiro Isao 
Published: March 2023

AbstractClinical trials on cancer treatments frequently exclude patients with prior cancer, but more evidence is needed to understand their possible effects on outcomes. This study analyzed the prognostic impact of prior cancer in newly diagnosed non-small cell lung cancer (NSCLC) patients while accounting for various patient and cancer characteristics. Using population-based cancer registry data linked with administrative claims data, this retrospective cohort study examined patients aged 15–84 years diagnosed with NSCLC between 2010 and 2015 in Japan. Cox proportional hazards models were used to estimate the hazard ratios (HRs) and 95% confidence intervals (CIs) of all-cause mortality in patients with versus without prior cancer. The analysis was stratified according to NSCLC stage and diagnostic time intervals between prior cancers and the index NSCLC. We analyzed 9103 patients (prior cancer: 1416 [15.6%]; no prior cancer: 7687 [84.4%]). Overall, prior cancer had a non-significant mortality HR of 1.07 (95% CI: 0.97–1.17). Furthermore, prior cancer had a significantly higher mortality hazard for diagnostic time intervals of 3 years (HR: 1.23, 95% CI: 1.06–1.43) and 5 years (1.18, 1.04–1.33), but not for longer intervals. However, prior cancer in patients with more advanced NSCLC did not show a higher mortality risk for any diagnostic time interval. Smoking-related prior cancers and prior cancers with poorer prognosis were associated with poorer survival. NSCLC patients with prior cancer do not have an invariably higher risk of mortality, and should be considered for inclusion in clinical trials depending on their cancer stage.
Numerical investigation of the effect of fluid pressurization rate on laboratory-scale injection-induced fault slip
Authors: Hutka Gergő András , Cacace Mauro , Hofmann Hannes , Zang Arno , Wang Lei , Ji Yinlin 
Published: March 2023

AbstractThe effect of normal stress variations on fault frictional strength has been extensively characterized in laboratory experiments and modelling studies based on a rate-and-state-dependent fault friction formalism. However, the role of pore pressure changes during injection-induced fault reactivation and associated frictional phenomena is still not well understood. We apply rate-and-state friction (RSF) theory in finite element models to investigate the effect of fluid pressurization rate on fault (re)activation and on the resulting frictional slip characteristics at the laboratory scale. We consider a stepwise injection scenario where each fluid injection cycle consists of a fluid pressurization phase followed by a constant fluid pressure phase. We first calibrate our model formulation to recently published laboratory results of injection-driven shear slip experiments. In a second stage, we perform a parametric study by varying fluid pressurization rates to cover a higher dimensional parameter space. We demonstrate that, for high permeability laboratory samples, the energy release rate associated with fault reactivation can be effectively controlled by a stepwise fluid injection scheme, i.e. by the applied fluid pressurization rate and the duration of the constant pressure phase between each successive fluid pressurization phase. We observe a gradual transition from fault creep to slow stick–slip as the fluid pressurization rate increases. Furthermore, computed peak velocities for an extended range of fluid pressurization rate scenarios (0.5 MPa/min to 10 MPa/min) indicate a non-linear (power-law) relationship between the imposed fluid pressurization rate and the peak slip velocities, and consequently with the energy release rate, for scenarios with a fluid pressurization rate higher than a critical value of 4 MPa/min. We also observe that higher pressurization rates cause a delay in the stress release by the fault. We therefore argue that by adopting a stepwise fluid injection scheme with lower fluid pressurization rates may provide the operator with a better control over potential induced seismicity. The implications for field-scale applications that we can derive from our study are limited by the high matrix and fault permeability of the selected sample and the direct hydraulic connection between the injection well and the fault, which may not necessarily represent the conditions typical for fracture dominated deep geothermal reservoirs. Nevertheless, our results can serve as a basis for further laboratory experiments and field-scale modelling studies focused on better understanding the impact of stepwise injection protocols on fluid injection-induced seismicity.
Exposure to local, source-specific ambient air pollution during pregnancy and autism in children: a cohort study from southern Sweden
Authors: Flanagan Erin , Malmqvist Ebba , Rittner Ralf , Gustafsson Peik , Källén Karin , Oudin Anna 
Published: March 2023

AbstractEvidence of air pollution exposure, namely, ambient particulate matter (PM), during pregnancy and an increased risk of autism in children is growing; however, the unique PM sources that contribute to this association are currently unknown. The aim of the present study was to investigate local, source-specific ambient PM exposure during pregnancy and its associations with childhood autism, specifically, and autism spectrum disorders (ASD) as a group. A cohort of 40,245 singleton births from 2000 to 2009 in Scania, Sweden, was combined with data on locally emitted PM with an aerodynamic diameter < 2.5 µm (PM2.5). A flat, two-dimensional dispersion model was used to assess local PM2.5 concentrations (all-source PM2.5, small-scale residential heating- mainly wood burning, tailpipe exhaust, and vehicle wear-and-tear) at the mother’s residential address during pregnancy. Associations were analyzed using binary logistic regression. Exposure to local PM2.5 during pregnancy from each of the investigated sources was associated with childhood autism in the fully adjusted models. For ASD, similar, but less pronounced, associations were found. The results add to existing evidence that exposure to air pollution during pregnancy may be associated with an increased risk of childhood autism. Further, these findings suggest that locally produced emissions from both residential wood burning and road traffic-related sources (tailpipe exhaust and vehicle wear-and-tear) contribute to this association.
Triple and double twin interfaces in magnesium—the role of disconnections and facets
Authors: Ruffino Martina , Nutter John , Zeng Xun , Guan Dikai , Rainforth W. Mark , Paxton Anthony T. 
Published: March 2023

AbstractTwin boundaries have been shown to deviate from the twinning planes in hcp metals, and facets have often been observed in twin interfaces. This study presents a twinning disconnection-based model for faceting in single, double and triple twin boundaries in magnesium. Primary twinning disconnections predicted via symmetry arguments are shown to produce commensurate facets in single twin boundaries, which are then transformed into commensurate facets in double twin boundaries via the action of secondary twinning disconnections. In contrast, it is shown that for triple twin boundaries with tension-compression-tension twinning sequence, no commensurate facets can be produced by the action of tertiary twinning disconnections. The effect of facets on the macroscopic orientation of twin interfaces is discussed. Theoretical findings are validated by a transmission electron microscopy study of a hot rolled Mg-1.18wt%Al-1.77wt%Nd alloy. Single and double twins are observed, as well as rare triple twins, and the interface between the matrix and a triple twin is captured for the first time. Facets consistent with theoretical predictions are imaged via high-resolution TEM and macroscopic deviations of the boundaries from the primary twinning planes are measured.
Nd:YAG infrared laser as a viable alternative to excimer laser: YBCO case study
Authors: Chaluvadi Sandeep Kumar , Punathum Chalil Shyni , Mazzola Federico , Dolabella Simone , Rajak Piu , Ferrara Marcello , Ciancio Regina , Fujii Jun , Panaccione Giancarlo , Rossi Giorgio , Orgiani Pasquale 
Published: March 2023

AbstractWe report on the growth and characterization of epitaxial YBa$$_{2}$$

2

Cu$$_{3}$$

3

O$$_{7-\delta }$$

7
-
δ

(YBCO) complex oxide thin films and related heterostructures exclusively by Pulsed Laser Deposition (PLD) and using first harmonic Nd:Y$$_{3}$$

3

Al$$_{5}$$

5

O$$_{12}$$

12

(Nd:YAG) pulsed laser source ($$\lambda$$
λ
= 1064 nm). High-quality epitaxial YBCO thin film heterostructures display superconducting properties with transition temperature $$\sim$$
∼
80 K. Compared with the excimer lasers, when using Nd:YAG lasers, the optimal growth conditions are achieved at a large target-to-substrate distance d. These results clearly demonstrate the potential use of the first harmonic Nd:YAG laser source as an alternative to the excimer lasers for the PLD thin film community. Its compactness as well as the absence of any safety issues related to poisonous gas represent a major breakthrough in the deposition of complex multi-element compounds in form of thin films.
A comprehensive analysis of PANoptosome to prognosis and immunotherapy response in pan-cancer
Authors: Zhuang Lingling , Sun Qiran , Huang Shenglan , Hu Lanyan , Chen Qi 
Published: March 2023

AbstractPANoptosis, a programmed cell death, shares key characteristics of apoptosis, pyroptosis, and necroptosis. Accumulating evidence suggests that PANoptosis plays a crucial role in tumorigenesis. However, the respective regulation mechanisms in cancer are so far unclear. Using various bioinformatic approaches, we comprehensively analyzed the expression patterns, genetic alterations, prognostic value, and immunological role of PANoptosis genes in pan-cancer. Expression of the PANoptosis gene, PYCARD, was validated based on the Human Protein Atlas database and real-time quantitative reverse transcription polymerase chain reaction (RT-PCR). We found that PANoptosis genes were aberrantly expressed in most cancer types, which was consistent with the validation of PYCARD expression. Concurrently, PANoptosis genes and PANoptosis scores were significantly associated with patient survival in 21 and 14 cancer types, respectively. Pathway analysis showed that PANoptosis score was positively correlated with pathways linked to immune and inflammatory responses in pan-cancer, such as IL6-JAK-STAT3 signaling, the interferon-gamma response, and IL2-STAT5 signaling. In addition, the PANoptosis score was significantly correlated with the tumor microenvironment, the infiltration levels of most immune cells (i.e.NK cells, CD8+ T cells, CD4+ T cells, DC cells), and immune-related genes. Furthermore, it was a predictive indicator of immunotherapy response in patients with tumors. These insights substantially improve our understanding of PANoptosis components in cancers and may inspire the discovery of novel prognostic and immunotherapy response biomarkers.
Understanding the microstructure of a core–shell anode catalyst layer for polymer electrolyte water electrolysis
Authors: De Angelis Salvatore , Schuler Tobias , Sabharwal Mayank , Holler Mirko , Guizar-Sicairos Manuel , Müller Elisabeth , Büchi Felix N. 
Published: March 2023

AbstractReducing precious metal loading in the anodic catalyst layer (CL) is indispensable for lowering capital costs and enabling the widespread adoption of polymer electrolyte water electrolysis. This work presents the first three-dimensional reconstruction of a TiO2-supported IrO2 based core shell CL (3 mgIrO2/cm2), using high-resolution X-ray ptychographic tomography at cryogenic temperature of 90 K. The high data quality and phase sensitivity of the technique have allowed the reconstruction of all four phases namely pore space, IrO2, TiO2 support matrix and the ionomer network, the latter of which has proven to be a challenge in the past. Results show that the IrO2 forms thin nanoporous shells around the TiO2 particles and that the ionomer has a non-uniform thickness and partially covers the catalyst. The TiO2 particles do not form a percolating network while all other phases have high connectivity. The analysis of the CL ionic and electronic conductivity shows that for a dry CL, the ionic conductivity is orders of magnitudes lower than the electronic conductivity. Varying the electronic conductivity of the support phase by simulations, reveals that the conductivity of the support does not have a considerable impact on the overall CL electrical conductivity.
A new monitoring system for nutritional status assessment in children at home
Authors: Zsakai Annamaria , Annar Dorina , Koronczai Beatrix , Molnar Kinga , Varro Petra , Toth Erika , Szarvas Szilvia , Tauber Tamas , Karkus Zsolt , Varnai Dora , Muzsnai Agota 
Published: March 2023

AbstractRegular monitoring of children’s nutritional status is essential to prevent micronutrient deficiencies, nutritional status abnormalities as stunting, wasting, overweight and obesity. Nutritional status assessment is usually performed by paediatricians by using anthropometry (body mass index, weight to height indices) and/or by body fat-mass measurement (bioimpedance analysis, dual-energy x-ray absorptiometry, computer tomography, etc.). Parents are also interested in but usually fail to evaluate their child’s nutritional status. To help the sufficient collaboration between the physician and parents a new nutritional status monitoring method is developed for families. The new monitoring system was developed under a paediatrician’s supervision by considering national and international recommendations, references as well as the anthropometric measurement possibilities at home. The model requires age, sex, body mass, height, waist circumference and hand circumference as predictor (input) variables of nutritional status, while (1) the centile values of the measured body dimensions, (2) body fat percentage and the centile of body fat percentage, (3) the nutritional status category (undernutrition, normal nutritional status, overfat/obese) can be predicted (outcome variables) by the new method. The predictive accuracy of the model for nutritional status category was 94.88% in boys and 98.66% in girls. The new model was developed for nutritional status assessment in school-aged children and will be incorporated in the healthy lifestyle module of ‘Teenage Survival Guide’ educational package to be developed by the Health Promotion and Education Research Team, Hungarian Academy of Sciences, Hungary. The new monitoring system could help the families to identify the early signs of malnutrition in children. Nutritional status assessment in children at home is suggested twice a year, and in case of suspicious nutritional status abnormality it is recommended to visit the general practitioner.
Possible species discrimination of a blotched nerite Nerita albicilla with their distribution pattern and demographic history in the Indo-Pacific
Authors: Hong Seonghyeon , Park Bia , Kim Gyeongmin , Choi Eun Hwa , Hwang Ui Wook 
Published: March 2023

AbstractThe blotched nerite Nerita albicilla (Linnaeus 1758) is distributed in intertidal areas of the Indo-Pacific. In South Korea, it has been found only in the southernmost region of Jeju Island so far. Owing to its limited distribution, it can be a promising intertidal species helpful for monitoring global climate change effects in the Korean Peninsula. We performed population genetic analyses based on 393 COI haplotypes from 697 N. albicilla, including 167 from this study and 530 from public databases. The results showed that there are two distinct genetic lineages in N. albicilla: PAIO (Palearctic, Australasia, Indo-Malay, and Oceania) and Afrotropic lineages. DNA barcoding gap analyses indicated that the two lineages could be differentiated into two different species: N. albicilla (PAIO) and N. originalis sp. nov. (Afrotropic) (3.96%). Additionally, it was revealed that their divergence time was ca. 5.96 Ma and dramatic diversification of COI haplotypes occurred during the late Pliocene and Pleistocene. The results of MDA, BSP, and neutrality test implied recent population size expansion, which was estimated to be ca. 250 Ka. Finally, we discussed whether the observation of N. originalis sp. nov. in South Korea is due to the northward migration through ocean currents caused by global warming or due to artificial activity through marine transportation.
Functional non-parametric mixed effects models for cytotoxicity assessment and clustering
Authors: Ma Tiantian , Richard Dan , Yang Yongqing Betty , Kashlak Adam B , Anton Cristina 
Published: March 2023

AbstractA multitude of natural and synthetic chemicals are present in our environment.Through the study of a compound’s cytotoxicity, researchers can carefully set regulations regarding how much of a certain chemical in the ambient environment is tolerable. In the past, research has focused on point measurements such as the LD50. Instead, we consider entire time-dependent cellular response curves through the application of functional mixed effects models. We identify differences in such curves corresponding to the chemical’s mode of action—i.e. how the compound attacks human cells. Through such analysis, we identify curve features to be used for cluster analysis via application of both k-means and self organizing maps. The data is analyzed by making use of functional principal components as a data driven basis and separately by considering B-splines for identifying local-time features. Our analysis can be used to drastically speed up future cytotoxicity research.