bluuebunny/crossref_metadata_2025_split huggingface dataset:
Biodegradation of isoproturon by Escherichia coli expressing a Pseudomonas putida catechol 1,2-dioxygenase gene
Authors: Elarabi Nagwa I. , Abdelhadi Abdelhadi A. , Nassrallah Amr A. , Mohamed Mahmoud S. M. , Abdelhaleem Heba A. R. 
Published: September 2023

AbstractThe phenylurea herbicides are persistent in soil and water, necessitating the creation of methods for removing them from the environment. This study aimed to examine the soil microbial diversity, searching for local bacterial isolates able to efficiently degrade the phenylurea herbicide isoproturon, 3-(4-isopropylphenyl)-1, 1-dimethylurea (IPU). The best isolates able to effectively degrade IPU were selected, characterized, and identified as Pseudomonas putida and Acinetobacter johnsonii. The catechol 1, 2-dioxygenase enzyme's catA gene was amplified, cloned, and expressed in E. coli M15. The Expressed E. coli showed high degradation efficiency (44.80%) as analyzed by HPLC after 15 days of inoculation in comparison to P. putida (21.60%). The expression of the catA gene in P. putida and expressed E. coli was measured using quantitative polymerase chain reaction (qPCR). The results displayed a significant increase in the mRNA levels of the catA gene by increasing the incubation time with IPU. Hydrophilic interaction chromatography (HILIC) mass spectrometry analysis revealed that three intermediate metabolites, 1-(4-isopropylphenyl)-3-methylurea (MDIPU), 4-Isopropylaniline (4-IA) and 1-(4-isopropylphenyl) urea (DDIPU) were generated by both P. putida and expressed E. coli. In addition, IPU-induced catA activity was detected in both P. putida and expressed E. coli. The supernatant of both P. putida and expressed E. coli had a significant influence on weed growth. The study clearly exhibited that P. putida and expressed E. coli were capable of metabolizing IPU influentially and thus could be utilized for bioremediation and biodegradation technology development.
Clinical impact of screening computed tomography in extracorporeal membrane oxygenation: a retrospective cohort study
Authors: Collins Patrick D. , Giosa Lorenzo , Kathar Sushil , Camarda Valentina , Palmesino Filippo , Eshwar Darshan , Barrett Nicholas A. , Retter Andrew , Vasques Francesco , Sanderson Barnaby , Mak Sze M. , Rose Louise , Camporota Luigi 
Published: September 2023

Abstract
Background
Data on the prevalence and clinical impact of extrapulmonary findings at screening computed tomography (CT) on initiation of veno-venous extracorporeal membrane oxygenation (V-V ECMO) are limited. We aimed to identify the prevalence of extrapulmonary findings on screening CT following V-V ECMO initiation. We hypothesized that extrapulmonary findings would influence clinical management and outcome.

Methods
Retrospective analysis (2011–2021) of admission screening CT including head, abdomen and pelvis with contrast of consecutive patients on initiation of V-V ECMO. CT findings identified by the attending consultant radiologist were extracted. Demographics, admission physiological and laboratory data, clinical decision-making following CT and ECMO ICU mortality were recorded from the electronic medical record. We used multivariable logistic regression and Kaplan–Meier curves to evaluate associations between extrapulmonary findings and ECMO ICU mortality.

Results
Of the 833 patients receiving V-V ECMO, 761 underwent routine admission CT (91.4%). ECMO ICU length of stay was 19 days (IQR 12–23); ICU mortality at the ECMO centre was 18.9%. An incidental extrapulmonary finding was reported in 227 patients (29.8%), leading to an invasive procedure in 12/227 cases (5.3%) and a change in medical management (mainly in anticoagulation strategy) in 119/227 (52.4%). Extrapulmonary findings associated with mortality were intracranial haemorrhage (OR 2.34 (95% CI 1.31–4.12), cerebral infarction (OR 3.59 (95% CI 1.26–9.86) and colitis (OR 2.80 (95% CI 1.35–5.67).

Conclusions
Screening CT frequently identifies extrapulmonary findings of clinical significance. Newly detected intracranial haemorrhage, cerebral infarction and colitis were associated with increased ICU mortality.
Animal tuberculosis control in a disease-free country, France: does the long and winding road really lead to eradication?
Authors: Boschiroli María Laura 
Published: September 2023

AbstractIt took France almost fifty years to attain its officially animal tuberculosis (TB) free status in 2000, granting the country a favourable position for international live animal trading. The initial TB control program has been adapted at different times in its history in order to suit changing epidemiological contexts: it was first focused on detection and elimination of infected animals while later on protecting TB free herds became a priority.In spite of all the efforts put into the program, final eradication has still not been achieved. Instead, the eradication process has stalled, most probably due to changes in breeding practices in the last 30 years. Indeed, the beef industry has overtaken the milk industry, which has led to the occurrence of new TB risks. Novel epidemiological situations in some regions of extensive beef cattle farming, where wildlife species (wild boar, badger) are also infected, have emerged. More adapted measures have thus been implemented, progressively evaluated and improved in order to reinforce prevention of infection, to follow up with the eradication goal and to strengthen, coordinate and re-motivate field resources. These include, among others, introduction of biosecurity measures in the herd, risk based surveillance and management of wildlife and cattle, improvement of screening in the field and at the abattoir, better diagnosis, but also improvement of communication, awareness, training activities of the main field actors. Very importantly, this new plan has been established through the participation of the majority of involved stakeholders -the farmer industry, hunter associations, veterinarians, scientists and the government-, through coordinated specific steering committees and ad hoc working groups.Without doubt, the main challenge for the next few years is reinforcing communication to encourage and strengthen the program in an already faltering agro-social system. In addition, it will be essential to continue sustaining national research and international collaborations to feed the program with relevant scientific data enabling the authorities to undertake the most pertinent measures for tackling the disease in the short term.
Evidence of anticipatory forest use behaviours under policy introduction: a systematic map protocol
Authors: Llopis Jorge Claudio , Haddaway Neal Robert , Omirbek Nurzhan , Simmons Blake Alexander , Garrett Rachael , Jones Julia Patricia Gordon 
Published: September 2023

AbstractBackgroundForest conservation is a major global policy goal, due to the role forests play in climate change mitigation and biodiversity conservation. It is well recognized that the introduction of policies, whether aimed at forest conservation or with other objectives, has the potential to trigger unintended outcomes, such as displacement or leakage, which can undermine policy objectives. However, a set of outcomes that has escaped detailed scrutiny are anticipatory forest use behaviours, emerging when forest stakeholders anticipate policy implementation, deploying for example pre-emptive forest clearing, resulting in detrimental environmental outcomes. Lack of understanding of the extent and sectorial scope of these behaviours prevents us from devising strategies to address their potential detrimental consequences.MethodsThis protocol presents the methodology that will be followed to conduct a systematic map to identify, compile, review and describe the evidence available on anticipatory forest use behaviours in the context of policy introduction around the world. We will use two complementary search strategies, which we have tested before submitting this protocol. First, a systematic bibliographic search, and second, a citation chase approach. We will include articles based on a pre-defined set of criteria defined according to a Population, Intervention and Outcome (i.e. PIO) design. To support identification of knowledge gaps and clusters, we will report results of the systematic map in a narrative synthesis, an evidence atlas and other visualisations.
Stability and Metastability of Li3YCl6 and Li3HoCl6
Authors: Ito Hiroaki , Nakahira Yuki , Ishimatsu Naoki , Goto Yosuke , Yamashita Aichi , Mizuguchi Yoshikazu , Moriyoshi Chikako , Toyao Takashi , Shimizu Ken-ichi , Oike Hiroshi , Enoki Masanori , Rosero-Navarro Nataly Carolina , Miura Akira , Tadanaga Kiyoharu 
Published: September 2023

Abstract
Metastable solid electrolytes exhibit superior conductivity compared to stable ones, making them a subject of considerable interest. However, synthesis of the metastable phase is affected by multiple thermodynamic and kinetic parameters, leading to ambiguity in the organization of stability and metastability. In this study, we organized remnant and intermediate metastability based on temperature. The intermediate metastable phase, which is less stable than the temperature-independent stable phase, typically transforms into the stable phase(s) at high temperatures. In contrast, the remnant metastable phase is formed by first obtaining most stable phase at specific temperatures and then “trapping” it by rapidly changing the temperature. By investigating Li+ conducting chlorides, Li3MCl6 (M = Y and Ho), we demonstrated that heating starting materials to approximately 600 K produced low-temperature Li3MCl6 phase with one formula unit while further heating resulted in high-temperature Li3MCl6 phase with three formula units. Annealing quenched Li3MCl6 at 573 K resulted in a phase transition from the high-temperature to low-temperature phase, indicating that the high-temperature phase was remnant metastable at low temperatures.
Diagnostic value of high-frequency ultrasonography for painful talocalcaneal coalition and its complications
Authors: Song Bing , Zhang Xiaohong , Wang Hongjun , Zhang Qinglin , Zhang Heng , Zhang Chengzheng 
Published: September 2023

Objective:
To investigate the value of high-frequency ultrasonography in the diagnosis of painful talocalcaneal coalition (TCC) and its complications.

Methods:
Seventy-seven patients (84 feet) with abnormal mass and pain in the medial malleolus were suspected of TCC pre-operatively and examined by high-frequency ultrasonography, radiograph, and CT. The sonographic characteristics of the affected feet were analyzed pre-operatively and compared with the surgical findings.

Results:
During the operation, 49 feet with TCC and 35 feet with non-TCC were confirmed; pre-operative ultrasonography diagnosed 48 feet with TCC and 36 feet with non-TCC; taking surgery as the gold-standard, the sensitivity, specificity, accuracy, positive-predictive value and negative-predictive value of ultrasound diagnosis of TCC were 87.8%, 85.7%, 86.9%, 89.6%, and 83.3%, respectively. The two were consistent, with κ = 0.732, p &lt; 0.001. High-frequency ultrasonography had high diagnostic efficacy for TCC, with an area under the receiver operating characteristic curve of 0.867. The accuracy of ultrasound and CT in the diagnosis of TCC was significantly higher than that of radiograph, and the difference was statistically significant (p &lt; 0.0167). High-frequency ultrasound could also accurately diagnose complications of TCC, such as tibial nerve compression and tendon displacement, while CT and radiograph cannot show these complications.

Conclusion:
High-frequency ultrasonography can accurately diagnose TCC and its complications, and locate the body surface accurately. Therefore, high-frequency ultrasonography can be used as a routine examination method to supplement CT and provide clinical assistance in precise surgery.

Advances in knowledge:
This study is the first to use high-frequency ultrasonography to examine TCC and compare its findings with surgical results to explore the diagnostic value of ultrasonography for TCC and its complications.
Altered morphological characteristics and structural covariance connectivity associated with verbal working memory performance in ADHD children
Authors: Liang Shengxiang , Huang Li , Zhan Shiqi , Zeng Yi , Zhang Qingqing , Zhang Yusi , Wang Xiuxiu , Peng Lixin , Lin Bohong , Xu Hui 
Published: September 2023

Objectives: Deficits in verbal working memory (VWM) observed in attention deficit hyperactivity disorder (ADHD) children can persist into adulthood. Although previous studies have identified brain regions that are activated during VWM tasks, the neural mechanisms underlying the relationship between VWM deficits remain unclear. The objective of this study was to investigate the structural covariance network connectivity and brain morphology changes that are associated with VWM performance in ADHD children. Methods: For this study, we selected 26 ADHD children and 26 healthy control (HC) participants. Participants were instructed to perform an n-back VWM task and their accuracy and response times were subsequently recorded. This research utilised voxel-based morphometry to measure the grey matter (GM) volume and conducted structural covariance connectivity network analysis to explore the changes of brain in ADHD. Results: Voxel-based morphometry analysis showed that lower GM volume in the right cerebellum lobule VI and the left parahippocampal gryus in ADHD children. Moreover, a positive correlation was found between the GM volume in the right cerebellum lobule VI and the accuracy of 2-back VWM task with verbal, small reward, and delayed feedback (VSD). Structural covariance network analysis found decreased structural connectivity between right cerebellum lobule VI and right precentral gyrus, right postcentral gyrus, left paracentral lobule, right superior parietal gyrus, and left hippocampus in ADHD children. Conclusions: The low GM volume and altered structural covariance connectivity in the right cerebellum lobule VI might potentially affect VWM performance in ADHD children. Advances in knowledge: The innovation of this study lies in its more focused discussion on the morphological characteristics and structural covariance connectivity of VWM deficits in ADHD children, and the innovative finding of a positive correlation between grey matter volume in the right cerebellum lobule VI and accuracy in completing the 2-back VWM task with verbal instructions, small reward, and delayed feedback (VSD). This expands upon previous research by elucidating the specific brain structures involved in VWM deficits in ADHD children and highlights the potential importance of the cerebellum in this cognitive process. Overall, these innovative findings advance our understanding of the neural basis of ADHD and may have important implications for the development of targeted interventions for VWM deficits.
Features of the state of neuro-endocrine factors of adaptation under different options of uric acid metabolism in healthy female rats
Authors: Popovych Igor , Bombushkar Igor , Badiuk Nataliya , Korda Inna , Zukow Walery , Gozhenko Anatoliy 
Published: September 2023

Background. As part of the project "Physiological activity of uric acid", we previously identified 4 variants-clusters of uricemia and uricosuria both in rats and in humans. It was shown that the clusters differ from each other in the constellation of immunity parameters, which to one degree or another correlate with uricemia and uricosuria. The aim of this study was to identify autonomic and endocrine adaptogenic factors, the combination of which differentiates previously formed quantitative and qualitative variants of uric acid metabolism in rats. Materials and Methods. Experiment was performed on 58 healthy female Wistar rats 220-300 g. The serum and urine levels of the uric acid, calcium, phosphates, sodium and potassium were determined. The neuro-endocrine status evaluated by the HRV parameters; serum levels of the hormones such as corticosterone, triiodothyronine and testosterone; daily excretion of 17-ketosteroides; electrolytes markers of mineralocorticoid, parathyroid and calcitonin activities as well as by the thickness of glomerular, fascicular, reticular and medullar zones of the adrenals. Results. The previously identified four variants-clusters of uric acid metabolism differ from each other by a constellation of 10 neuro-endocrine adaptation factors that reflect the state of glucocorticoid, androgenic and catecholamine functions of the adrenal glands, as well as HRV-marker of vagal tone and electrolyte markers of sympatho-vagal balance as well as calcitonin and parathyroid activities. Classification accuracy is 90%. Uricosuria and uricemia are negatively correlated with HRV-markers of sympathetic tone and circulating catecholamines, the level of corticosterone and the thickness of the fascicular zone of the adrenal cortex secreting them, as well as the level of triiodothyronine and the Ca-P marker of calcitonin activity, on the other hand, they are positively correlated with the HRV marker of vagal tone and urinary excretion of 17-ketosteroids. The rate of determination of neuro-endocrine adaptation factors by uric acid is 62%. Conclusion. Uric acid is naturally associated with the state of autonomous and endocrine adaptation factors in healthy female rats.
Paraneoplastic syndromes in dermatology - cutaneous manifestations of internal malignancy
Authors: Matyja Karolina , Berner Aleksandra , Stencel Katarzyna , Pękała Maciej , Olszewska Anna , Stelmaszak Karina , Marczyk Klaudia , Polak Paulina , Polaszek Monika , Bogowska Marta 
Published: September 2023

Introduction: Cutaneous manifestations linked with internal malignancies can exhibit a wide range of appearances.The ideal paraneoplastic marker is one that exclusively appears with a tumor, vanishes upon tumor treatment, and can potentially reemerge with tumor recurrence or metastasis. They are divided into two main categories: acquired and genetic conditions. Among acquired paraneoplastic syndromes a few are strongly correlated with internal malignancies, others have less pronounced associations.
Aim of the study: To review the current literature on the topic of dermatological paraneoplastic syndromes.
Materials and Methods: We reviewed the literature available in PubMed, using the key words: “paraneoplastic syndromes”, “paraneoplastic dermatoses”, “paraneoplastic syndromes in dermatology”.
Results: We presented the most common, selected skin paraneoplastic syndromes. We indicated the types of neoplasms associated with these dermatoses and described their clinical appearance along with potential mechanisms of development.
Summary: Timely recognition of paraneoplastic skin manifestations is crucial as they can provide an opportunity for diagnosing and treating the underlying neoplasm. That’s why dermatologists and clinicians should be familiar with this topic to perform an early diagnosis of the underlying malignancy.
Impact of COVID-19 on the functioning of medical facilities in Poland
Authors: Kaźmierkiewicz Kornelia , Smulewicz Klaudia , Wójcik Alicja , Ziomko Bartłomiej 
Published: September 2023

The COVID-19 pandemic in Poland lasted from March 2020 to May 2022. As a result of the new strain of the virus, it was necessary to change the way primary health care units work. In order to allow patients to interact with medical staff, teleconsultations were introduced. This was thought to reduce the spread of the virus and reduce the risk of contracting SARS-CoV-2. Many other countries have seen a reduction in in-person visits and an increase in online doctor consultations. The aim of the study was to examine the level of services provided by medical facilities, including efficiency, effectiveness and patient satisfaction. The survey, which included 246 people, showed that most respondents used remote visits during the pandemic and more than 50% of them believe that clinics were not adequately prepared for the epidemiological situation. Despite the rapid development of technology in the medical sector, patients still prefer the traditional form of medical consultation, which they argue is more accurate and insightful for the doctor during this type of visit.
Buffer Times Between Scheduled Events in Resource Assignment Problem: A Conflict-Robust Perspective
Authors: Huang Jinjia , Teo Chung-Piaw , Wang Fan , Xu Zhou 
Published: September 2023

Problem definition: In many resource scheduling problems for services with scheduled starting and completion times (e.g., airport gate assignment), a common approach is to maintain appropriate buffer between successive services assigned to a common resource. With a large buffer, the chances of a “crossing” (i.e., a flight arriving later than the succeeding one at the gate) will be significantly reduced. This approach is often preferred over more sophisticated stochastic mixed-integer programming methods that track the arrival of all the flights to infer the number of “conflicts” (i.e., a flight arriving at a time when the assigned gate becomes unavailable). We provide a theoretical explanation, from the perspective of robust optimization for the good performance of the buffering approach in minimizing not only the number of crossings but also the number of conflicts in the operations. Methodology/results: We show that the buffering method inherently minimizes the worst-case number of “conflicts” under both robust and distributionally robust optimization models using down-monotone uncertainty sets. Interestingly, under down-monotone properties, the worst-case number of crossings is identical to the worst-case number of conflicts. Using this equivalence, we demonstrate how feature information from flight and historical delay information can be used to enhance the effectiveness of the buffering method. Managerial implications: The paper provides the first theoretical justification on the use of buffering method to control for the number of conflicts in resource assignment problem. Funding: This work was supported by the 2019 Academic Research Fund Tier 3 of the Ministry of Education-Singapore [Grant MOE-2019-T3-1-010] and the Research Grants Council of Hong Kong SAR, China [Grant PolyU 152240/17E]. Supplemental Material: The online appendix is available at https://doi.org/10.1287/msom.2022.0572 .
Professional responsibility in obstetric-gynecological nursing: Update of the case law of the High Court of Justice of Andalusia (Spain)
Authors: González-Hernández Luz Marina , San José Jorge 
Published: September 2023

Background
Obstetric-Gynecological Nursing is articulated as one of the specialities with the greatest responsibility in the field of health care, due to the involvement of being in care not only for the life of the pregnant woman, but also for the future neonate. Settling down as a profession with a high number of legal claims, there are not many studies in Spain on legal claims in the field of Nursing in general, and Obstetric-Gynecological Nursing in particular.

Methods
Retrospective analysis of judgments against midwives in the period from 2010 to the present through the CENDOJ database, with the aim of searching for criminal, civil and contentious-administrative judgments. Quality was assessed using the STROBE critical appraisal tool for observational studies.

Results
19 judgments were selected from the 197 found that were related to the objective of the study. Fifty-three percent of the judgment analyzed resulted in acquittals, while the remaining 47% were upheld to varying degrees. Most of them were motivated damage to the newborn (79%), processed entirely through the contentious-administrative route.

Conclusions
Most of the legal claims in the field of Obstetric-Gynecological Nursing are related to adverse events with fetal damage, most of them receiving higher monetary compensation as the contentious-administrative route is the jurisdiction with the highest number of claims filed, due to breaches of the lex artis ad hoc.