bluuebunny/crossref_metadata_2025_split huggingface dataset:
Global Formaldehyde Products From the Ozone Mapping and Profiler Suite (OMPS) Nadir Mappers on Suomi NPP and NOAA‐20
Authors: Nowlan Caroline R. , González Abad Gonzalo , Kwon Hyeong‐Ahn , Ayazpour Zolal , Chan Miller Christopher , Chance Kelly , Chong Heesung , Liu Xiong , O’Sullivan Ewan , Wang Huiqun , Zhu Lei , De Smedt Isabelle , Jaross Glen , Seftor Colin , Sun Kang 
Published: May 2023

AbstractWe describe new publicly available, multi‐year formaldehyde (HCHO) data records from the Ozone Mapping and Profiler Suite (OMPS) nadir mapper (NM) instruments on the Suomi NPP and NOAA‐20 satellites. The OMPS‐NM instruments measure backscattered UV light over the globe once per day, with spatial resolutions close to nadir of 50 × 50 km2 (OMPS/Suomi‐NPP) and 17 × 17 km2 or 12 × 17 km2 (OMPS/NOAA‐20). After a preliminary instrument line shape and wavelength calibration using on‐orbit observations, we use the backscatter measurements in a direct spectral fit of radiances, in combination with a nadir reference spectrum collected over a clean area, to determine slant columns of HCHO. The slant columns are converted to vertical columns using air mass factors (AMFs) derived through scene‐by‐scene radiative transfer calculations. Finally, a correction is applied to account for background HCHO in the reference spectrum, as well as any remaining high‐latitude biases. We investigate the consistency of the OMPS products from Suomi NPP and NOAA‐20 using long‐term monthly means over 12 geographic regions, and also compare the products with publicly available TROPOMI HCHO observations. OMPS/Suomi‐NPP and OMPS/NOAA‐20 monthly mean HCHO vertical columns are highly consistent (r = 0.98), with low proportional (2%) and offset (2 × 1014 molecules cm−2) biases. OMPS HCHO monthly means are also well‐correlated with those from TROPOMI (r = 0.92), although they are consistently 10% ± 16% larger in polluted regions (columns >8 × 1015 molecules cm−2). These differences result primarily from differences in AMFs.
Projected Changes in the Pattern of Spatially Compounding Drought and Pluvial Events Over Eastern China Under a Warming Climate
Authors: Chen Huijiao , Wang Shuo , Zhu Jinxin , Wang Dagang 
Published: May 2023

AbstractThe simultaneous occurrence of droughts and floods in neighboring regions amplifies the threats posed by droughts and floods individually. Nonetheless, few studies have been conducted to investigate the simultaneous occurrence of drought and flood events. Here we explore the spatiotemporal characteristics and the shift pattern of droughts and pluvials over Eastern China from a three‐dimensional perspective, using the self‐calibrated Palmer Drought Severity Index and the Climate Research Unit data set as well as four regional climate model simulations. We find that Eastern China experienced droughts and pluvials simultaneously in different locations during boreal summer, and it is projected to simultaneously experience more frequent and more intense droughts and pluvials under a warming climate. Specifically, we investigate the pattern of more pluvials in Southeast China and more droughts in Northeast China for the historical period of 1975–2004. This pattern dynamically evolves under climate warming: the pluvial‐dominated regime shifts from Southeast to Northeast China, while the drought‐dominated regime shifts from Northeast to Southeast China. The weakening strength of the western Pacific subtropical high and a northward displacement of the monsoon rain belt may both contribute to the pattern of more pluvials in Northeast China and more droughts in Southeast China. These findings provide insights into the development of adaptation strategies and emergency response plans for enhancing society's resilience to the spatial co‐occurrence of dry and wet extremes.
Rational adjustment to interfacial interaction with carbonized polymer dots enabling efficient large-area perovskite light-emitting diodes
Authors: Yang Fan , Zeng Qingsen , Dong Wei , Kang Chunyuan , Qu Zexing , Zhao Yue , Wei Haotong , Zheng Weitao , Zhang Xiaoyu , Yang Bai 
Published: May 2023

AbstractFilm uniformity of solution-processed layers is the cornerstone of large-area perovskite light-emitting diodes, which is often determined by the ‘coffee-ring effect’. Here we demonstrate a second factor that cannot be ignored is the solid-liquid interface interaction between substrate and precursor and can be optimized to eliminate rings. A perovskite film with rings can be formed when cations dominate the solid-liquid interface interaction; whereas smooth and homogeneous perovskite emitting layers are generated when anions and anion groups dominate the interaction. This is due to the fact that the type of ions anchored to the substrate can determine how the subsequent film grows. This interfacial interaction is adjusted using carbonized polymer dots, who also orient the perovskite crystals and passivate their buried traps, enabling a 225 mm2 large-area perovskite light-emitting diode with a high efficiency of 20.2%.
Suppressing fear in the presence of a safety cue requires infralimbic cortical signaling to central amygdala
Authors: Ng Ka , Pollock Michael , Escobedo Abraham , Bachman Brent , Miyazaki Nanami , Bartlett Edward L. , Sangha Susan 
Published: May 2023

AbstractStressful events can have lasting and impactful effects on behavior, especially by disrupting normal regulation of fear and reward processing. Accurate discrimination among environmental cues predicting threat, safety or reward adaptively guides behavior. Post-traumatic stress disorder (PTSD) represents a condition in which maladaptive fear persists in response to explicit safety-predictive cues that coincide with previously learned threat cues, but without threat being present. Since both the infralimbic cortex (IL) and amygdala have each been shown to be important for fear regulation to safety cues, we tested the necessity of specific IL projections to the basolateral amygdala (BLA) or central amygdala (CeA) during safety recall. Male Long Evans rats were used since prior work showed female Long Evans rats did not acquire the safety discrimination task used in this study. Here, we show the infralimbic projection to the central amygdala was necessary for suppressing fear cue-induced freezing in the presence of a learned safety cue, and the projection to the basolateral amygdala was not. The loss of discriminative fear regulation seen specifically during IL->CeA inhibition is similar to the behavioral disruption seen in PTSD individuals that fail to regulate fear in the presence of a safety cue.
Dual role of ANGPTL8 in promoting tumor cell proliferation and immune escape during hepatocarcinogenesis
Authors: Gao Yujiu , Yuan Yue , Wen Shu , Chen Yanghui , Zhang Zongli , Feng Ying , Jiang Bin , Ma Shinan , Hu Rong , Fang Chen , Ruan Xuzhi , Yuan Yahong , Fang Xinggang , Luo Chao , Meng Zhongji , Wang Xiaoli , Guo Xingrong 
Published: May 2023

AbstractThe interplay between hepatocellular carcinoma (HCC) cells and the tumor microenvironment is essential for hepatocarcinogenesis, but their contributions to HCC development are incompletely understood. We assessed the role of ANGPTL8, a protein secreted by HCC cells, in hepatocarcinogenesis and the mechanisms through which ANGPTL8 mediates crosstalk between HCC cells and tumor-associated macrophages. Immunohistochemical, Western blotting, RNA-Seq, and flow cytometry analyses of ANGPTL8 were performed. A series of in vitro and in vivo experiments were conducted to reveal the role of ANGPTL8 in the progression of HCC. ANGPTL8 expression was positively correlated with tumor malignancy in HCC, and high ANGPTL8 expression was associated with poor overall survival (OS) and disease-free survival (DFS). ANGPTL8 promoted HCC cell proliferation in vitro and in vivo, and ANGPTL8 KO inhibited the development of HCC in both DEN-induced and DEN-plus-CCL4-induced mouse HCC tumors. Mechanistically, the ANGPTL8–LILRB2/PIRB interaction promoted polarization of macrophages to the immunosuppressive M2 phenotype in macrophages and recruited immunosuppressive T cells. In hepatocytes, ANGPTL8-mediated stimulation of LILRB2/PIRB regulated the ROS/ERK pathway and upregulated autophagy, leading to the proliferation of HCC cells. Our data support the notion that ANGPTL8 has a dual role in promoting tumor cell proliferation and immune escape during hepatocarcinogenesis.
SYK-mediated epithelial cell state is associated with response to c-Met inhibitors in c-Met-overexpressing lung cancer
Authors: Zhou Ji , Zhang Xu-Chao , Xue Shan , Dai Mengdi , Wang Yueliang , Peng Xia , Chen Jianjiao , Wang Xinyi , Shen Yanyan , Qin Hui , Chen Bi , Zheng Yu , Gao Xiwen , Xie Zuoquan , Ding Jian , Jiang Handong , Wu Yi-Long , Geng Meiyu , Ai Jing 
Published: May 2023

AbstractGenomic MET amplification and exon 14 skipping are currently clinically recognized biomarkers for stratifying subsets of non-small cell lung cancer (NSCLC) patients according to the predicted response to c-Met inhibitors (c-Metis), yet the overall clinical benefit of this strategy is quite limited. Notably, c-Met protein overexpression, which occurs in approximately 20–25% of NSCLC patients, has not yet been clearly defined as a clinically useful biomarker. An optimized strategy for accurately classifying patients with c-Met overexpression for decision-making regarding c-Meti treatment is lacking. Herein, we found that SYK regulates the plasticity of cells in an epithelial state and is associated with their sensitivity to c-Metis both in vitro and in vivo in PDX models with c-Met overexpression regardless of MET gene status. Furthermore, TGF-β1 treatment resulted in SYK transcriptional downregulation, increased Sp1-mediated transcription of FRA1, and restored the mesenchymal state, which conferred resistance to c-Metis. Clinically, a subpopulation of NSCLC patients with c-Met overexpression coupled with SYK overexpression exhibited a high response rate of 73.3% and longer progression-free survival with c-Meti treatment than other patients. SYK negativity coupled with TGF-β1 positivity conferred de novo and acquired resistance. In summary, SYK regulates cell plasticity toward a therapy-sensitive epithelial cell state. Furthermore, our findings showed that SYK overexpression can aid in precisely stratifying NSCLC patients with c-Met overexpression regardless of MET alterations and expand the population predicted to benefit from c-Met-targeted therapy.
Hypoxia-mediated promotion of glucose metabolism in non-small cell lung cancer correlates with activation of the EZH2/FBXL7/PFKFB4 axis
Authors: Zhou Jing , Lin Yang , Kang Xiuhua , Liu Zhicheng , Zou Juntao , Xu Fei 
Published: May 2023

AbstractF-box/LRR-repeat protein 7 (FBXL7) was predicted as a differentially expressed E3 ubiquitin ligase in non-small cell lung cancer (NSCLC), which has been suggested to influence cancer growth and metastasis. In this study, we aimed to decipher the function of FBXL7 in NSCLC and delineate the upstream and downstream mechanisms. FBXL7 expression was verified in NSCLC cell lines and GEPIA-based tissue samples, after which the upstream transcription factor of FBXL7 was bioinformatically identified. The substrate PFKFB4 of the FBXL7 was screened out by tandem affinity purification coupled with mass-spectrometry (TAP/MS). FBXL7 was downregulated in NSCLC cell lines and tissue samples. FBXL7 ubiquitinated and degraded PFKFB4, thus suppressing glucose metabolism and malignant phenotypes of NSCLC cells. Hypoxia-induced HIF-1α upregulation elevated EZH2 and inhibited FBXL7 transcription and reduced its expression, thus promoting PFKFB4 protein stability. By this mechanism, glucose metabolism and the malignant phenotype were enhanced. In addition, knockdown of EZH2 impeded tumor growth through the FBXL7/PFKFB4 axis. In conclusion, our work reveals that the EZH2/FBXL7/PFKFB4 axis plays a regulatory role in glucose metabolism and tumor growth of NSCLC, which is expected to be potential biomarkers for NSCLC.
IGF2BP3 promotes adult myocardial regeneration by stabilizing MMP3 mRNA through interaction with m6A modification
Authors: Li Simeng , Shen Siman , Xu Hao , Cai Shuyun , Yuan Xiaodong , Wang Changsen , Zhang Xiaojun , Chen Suyun , Chen Jianning , Shi De-Li , Zhang Liangqing 
Published: May 2023

AbstractMyocardial infarction that causes damage to heart muscle can lead to heart failure. The identification of molecular mechanisms promoting myocardial regeneration represents a promising strategy to improve cardiac function. Here we show that IGF2BP3 plays an important role in regulating adult cardiomyocyte proliferation and regeneration in a mouse model of myocardial infarction. IGF2BP3 expression progressively decreases during postnatal development and becomes undetectable in the adult heart. However, it becomes upregulated after cardiac injury. Both gain- and loss-of-function analyses indicate that IGF2BP3 regulates cardiomyocyte proliferation in vitro and in vivo. In particular, IGF2BP3 promotes cardiac regeneration and improves cardiac function after myocardial infarction. Mechanistically, we demonstrate that IGF2BP3 binds to and stabilizes MMP3 mRNA through interaction with N6-methyladenosine modification. The expression of MMP3 protein is also progressively downregulated during postnatal development. Functional analyses indicate that MMP3 acts downstream of IGF2BP3 to regulate cardiomyocyte proliferation. These results suggest that IGF2BP3-mediated post-transcriptional regulation of extracellular matrix and tissue remodeling contributes to cardiomyocyte regeneration. They should help to define therapeutic strategy for ameliorating myocardial infarction by inducing cell proliferation and heart repair.
Prioritization of potential causative genes for schizophrenia in placenta
Authors: Ursini Gianluca , Di Carlo Pasquale , Mukherjee Sreya , Chen Qiang , Han Shizhong , Kim Jiyoung , Deyssenroth Maya , Marsit Carmen J. , Chen Jia , Hao Ke , Punzi Giovanna , Weinberger Daniel R. 
Published: May 2023

AbstractOur earlier work has shown that genomic risk for schizophrenia converges with early life complications in affecting risk for the disorder and sex-biased neurodevelopmental trajectories. Here, we identify specific genes and potential mechanisms that, in placenta, may mediate such outcomes. We performed TWAS in healthy term placentae (N = 147) to derive candidate placental causal genes that we confirmed with SMR; to search for placenta and schizophrenia-specific associations, we performed an analogous analysis in fetal brain (N = 166) and additional placenta TWAS for other disorders/traits. The analyses in the whole sample and stratifying by sex ultimately highlight 139 placenta and schizophrenia-specific risk genes, many being sex-biased; the candidate molecular mechanisms converge on the nutrient-sensing capabilities of placenta and trophoblast invasiveness. These genes also implicate the Coronavirus-pathogenesis pathway and showed increased expression in placentae from a small sample of SARS-CoV-2-positive pregnancies. Investigating placental risk genes for schizophrenia and candidate mechanisms may lead to opportunities for prevention that would not be suggested by study of the brain alone.
Flattened 1D fragments of fullerene C60 that exhibit robustness toward multi-electron reduction
Authors: Hayakawa Masahiro , Sunayama Naoyuki , Takagi Shu I. , Matsuo Yu , Tamaki Asuka , Yamaguchi Shigehiro , Seki Shu , Fukazawa Aiko 
Published: May 2023

AbstractFullerenes are compelling molecular materials owing to their exceptional robustness toward multi-electron reduction. Although scientists have attempted to address this feature by synthesizing various fragment molecules, the origin of this electron affinity remains unclear. Several structural factors have been suggested, including high symmetry, pyramidalized carbon atoms, and five-membered ring substructures. To elucidate the role of the five-membered ring substructures without the influence of high symmetry and pyramidalized carbon atoms, we herein report the synthesis and electron-accepting properties of oligo(biindenylidene)s, a flattened one-dimensional fragment of fullerene C60. Electrochemical studies corroborated that oligo(biindenylidene)s can accept electrons up to equal to the number of five-membered rings in their main chains. Moreover, ultraviolet/visible/near-infrared absorption spectroscopy revealed that oligo(biindenylidene)s exhibit enhanced absorption covering the entire visible region relative to C60. These results highlight the significance of the pentagonal substructure for attaining stability toward multi-electron reduction and provide a strategy for the molecular design of electron-accepting π-conjugated hydrocarbons even without electron-withdrawing groups.
Parasitic capacitance modeling and measurements of conductive yarns for e-textile devices
Authors: Qu Ziqi , Zhu Zhechen , Liu Yulong , Yu Mengxia , Ye Terry Tao 
Published: May 2023

AbstractConductive yarns have emerged as a viable alternative to metallic wires in e-Textile devices, such as antennas, inductors, interconnects, and more, which are integral components of smart clothing applications. But the parasitic capacitance induced by their micro-structure has not been fully understood. This capacitance greatly affects device performance in high-frequency applications. We propose a lump-sum and turn-to-turn model of an air-core helical inductor constructed from conductive yarns, and systematically analyze and quantify the parasitic elements of conductive yarns. Using three commercial conductive yarns as examples, we compare the frequency response of copper-based and yarn-based inductors with identical structures to extract the parasitic capacitance. Our measurements show that the unit-length parasitic capacitance of commercial conductive yarns ranges from 1 fF/cm to 3 fF/cm, depending on the yarn’s microstructure. These measurements offer significant quantitative estimation of conductive yarn parasitic elements and provide valuable design and characterization guidelines for e-Textile devices.
RagD auto-activating mutations impair MiT/TFE activity in kidney tubulopathy and cardiomyopathy syndrome
Authors: Sambri Irene , Ferniani Marco , Campostrini Giulia , Testa Marialuisa , Meraviglia Viviana , de Araujo Mariana E. G. , Dokládal Ladislav , Vilardo Claudia , Monfregola Jlenia , Zampelli Nicolina , Vecchio Blanco Francesca Del , Torella Annalaura , Ruosi Carolina , Fecarotta Simona , Parenti Giancarlo , Staiano Leopoldo , Bellin Milena , Huber Lukas A. , De Virgilio Claudio , Trepiccione Francesco , Nigro Vincenzo , Ballabio Andrea 
Published: May 2023

AbstractHeterozygous mutations in the gene encoding RagD GTPase were shown to cause a novel autosomal dominant condition characterized by kidney tubulopathy and cardiomyopathy. We previously demonstrated that RagD, and its paralogue RagC, mediate a non-canonical mTORC1 signaling pathway that inhibits the activity of TFEB and TFE3, transcription factors of the MiT/TFE family and master regulators of lysosomal biogenesis and autophagy. Here we show that RagD mutations causing kidney tubulopathy and cardiomyopathy are “auto- activating”, even in the absence of Folliculin, the GAP responsible for RagC/D activation, and cause constitutive phosphorylation of TFEB and TFE3 by mTORC1, without affecting the phosphorylation of “canonical” mTORC1 substrates, such as S6K. By using HeLa and HK-2 cell lines, human induced pluripotent stem cell-derived cardiomyocytes and patient-derived primary fibroblasts, we show thatRRAGDauto-activating mutations lead to inhibition of TFEB and TFE3 nuclear translocation and transcriptional activity, which impairs the response to lysosomal and mitochondrial injury. These data suggest that inhibition of MiT/TFE factors plays a key role in kidney tubulopathy and cardiomyopathy syndrome.
Structural insights into cardiolipin replacement by phosphatidylglycerol in a cardiolipin-lacking yeast respiratory supercomplex
Authors: Hryc Corey F. , Mallampalli Venkata K. P. S. , Bovshik Evgeniy I. , Azinas Stavros , Fan Guizhen , Serysheva Irina I. , Sparagna Genevieve C. , Baker Matthew L. , Mileykovskaya Eugenia , Dowhan William 
Published: May 2023

AbstractCardiolipin is a hallmark phospholipid of mitochondrial membranes. Despite established significance of cardiolipin in supporting respiratory supercomplex organization, a mechanistic understanding of this lipid-protein interaction is still lacking. To address the essential role of cardiolipin in supercomplex organization, we report cryo-EM structures of a wild type supercomplex (IV1III2IV1) and a supercomplex (III2IV1) isolated from a cardiolipin-lacking Saccharomyces cerevisiae mutant at 3.2-Å and 3.3-Å resolution, respectively, and demonstrate that phosphatidylglycerol in III2IV1 occupies similar positions as cardiolipin in IV1III2IV1. Lipid-protein interactions within these complexes differ, which conceivably underlies the reduced level of IV1III2IV1 and high levels of III2IV1 and free III2 and IV in mutant mitochondria. Here we show that anionic phospholipids interact with positive amino acids and appear to nucleate a phospholipid domain at the interface between the individual complexes, which dampen charge repulsion and further stabilize interaction, respectively, between individual complexes.
Pan-sarbecovirus prophylaxis with human anti-ACE2 monoclonal antibodies
Authors: Zhang Fengwen , Jenkins Jesse , de Carvalho Renan V. H. , Nakandakari-Higa Sandra , Chen Teresia , Abernathy Morgan E. , Baharani Viren A. , Nyakatura Elisabeth K. , Andrew David , Lebedeva Irina V. , Lorenz Ivo C. , Hoffmann H.-Heinrich , Rice Charles M. , Victora Gabriel D. , Barnes Christopher O. , Hatziioannou Theodora , Bieniasz Paul D. 
Published: May 2023

AbstractHuman monoclonal antibodies (mAbs) that target the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike protein have been isolated from convalescent individuals and developed into therapeutics for SARS-CoV-2 infection. However, therapeutic mAbs for SARS-CoV-2 have been rendered obsolete by the emergence of mAb-resistant virus variants. Here we report the generation of a set of six human mAbs that bind the human angiotensin-converting enzyme-2 (hACE2) receptor, rather than the SARS-CoV-2 spike protein. We show that these antibodies block infection by all hACE2 binding sarbecoviruses tested, including SARS-CoV-2 ancestral, Delta and Omicron variants at concentrations of ~7–100 ng ml−1. These antibodies target an hACE2 epitope that binds to the SARS-CoV-2 spike, but they do not inhibit hACE2 enzymatic activity nor do they induce cell-surface depletion of hACE2. They have favourable pharmacology, protect hACE2 knock-in mice against SARS-CoV-2 infection and should present a high genetic barrier to the acquisition of resistance. These antibodies should be useful prophylactic and treatment agents against any current or future SARS-CoV-2 variants and might be useful to treat infection with any hACE2-binding sarbecoviruses that emerge in the future.