bluuebunny/crossref_metadata_2025_split huggingface dataset:
Sphingosine 1-phosphate receptor 2 promotes the onset and progression of non-alcoholic fatty liver disease-related hepatocellular carcinoma through the PI3K/AKT/mTOR pathway
Authors: Wang Ganggang , Zhang Xin , Zhou Zhijie , Song Chao , Jin Wenzhi , Zhang Hao , Wu Weixin , Yi Yong , Cui Hengguan , Zhang Ping , Liu Xinyu , Xu Weiqiang , Shen Xiaowei , Shen Weixing , Wang Xiaoliang 
Published: January 2023

Abstract
Purpose
Recent studies have revealed an increase in the incidence rate of non-alcoholic fatty liver disease-related hepatocellular carcinoma (NAFLD-HCC). Furthermore, the association of Sphingosine 1-phosphate receptor 2 (S1PR2) with various types of tumours is identified, and the metabolism of conjugated bile acids (CBAs) performs an essential function in the onset and development of HCC. However, the association of CBA and S1PR2 with NAFLD-HCC is unclear.

Methods
The relationship between the expression of S1PR2 and the prognosis of patients suffering from NAFLD-HCC was investigated by bioinformatics techniques. Subsequently, the relationship between S1PR2 and the biological behaviours of HCC cell lines Huh 7 and HepG2 was explored by conducting molecular biology assays. Additionally, several in vivo animal experiments were carried out for the elucidation of the biological impacts of S1PR2 inhibitors on HCC cells. Finally, We used Glycodeoxycholic acid (GCDA) of CBA to explore the biological effects of CBA on HCC cell and its potential mechanism.

Results
High S1PR2 expression was linked to poor prognosis of the NAFLD-HCC patients. According to cellular assay results, S1PR2 expression could affect the proliferation, invasion, migration, and apoptosis of Huh 7 and HepG2 cells, and was closely associated with the G1/G2 phase of the cell cycle. The experiments conducted in the In vivo conditions revealed that the overexpression of S1PR2 accelerated the growth of subcutaneous tumours. In addition, JTE-013, an antagonist of S1PR2, effectively inhibited the migration and proliferation of HCC cells. Furthermore, the bioinformatics analysis highlighted a correlation between S1PR2 and the PI3K/AKT/mTOR pathway.
GCDA administration further enhanced the expression levels of p-AKT, p-mTOR, VEGF, SGK1, and PKCα. Moreover, both the presence and absence of GCDA did not reveal any significant change in the levels of S1PR2, p-AKT, p-mTOR, VEGF, SGK1, and PKCα proteins under S1PR2 knockdown, indicating that CBA may regulates the PI3K/AKT/mTOR pathway by mediating S1PR2 expression.

Conclusion
S1PR2 is a potential prognostic biomarker in NAFLD-HCC. In addition, We used GCDA in CBAs to treat HCC cell and found that the expression of S1PR2 was significantly increased, and the expression of PI3K/AKT/mTOR signalling pathway-related signal molecules was also significantly enhanced, indicating that GCDA may activate PI3K/AKT/mTOR signalling pathway by up-regulating the expression of S1PR2, and finally affect the activity of hepatocellular carcinoma cells. S1PR2 can be a candidate therapeutic target for NAFLD-HCC. Collectively, the findings of this research offer novel perspectives on the prevention and treatment of NAFLD-HCC.
Developing Culturally Tailored Mobile Web App Education to Promote Breast Cancer Screening: Knowledge, Barriers, and Needs Among American Indian Women
Authors: Roh Soonhee , Lee Yeon-Shim 
Published: January 2023

Abstract
American Indian (AI) women face disproportionate rates of breast cancer mortality and cancer disparities. This study conducted qualitative research to assess perspectives of AI women towards breast cancer screening, knowledge, barriers, and needs about mobile web app–based education to promote breast cancer screening. This study, in collaboration with the Yankton Sioux Tribe (YST), followed a community-based participatory research approach and conducted two focus groups with a total of 22 YST women aged 40–70 years living on reservation in rural South Dakota. Each group consisted of 11 local professionals working in healthcare and social services and community members. A grounded theory was used for the qualitative analysis. A large portion of participants reported having prior knowledge about breast cancer and screening methods, yet lacked awareness of the detailed procedure and recommended guidelines. Competing priorities and cost of mammograms were noted as major barriers to screening. Participants wanted to learn—in a convenient and easy-to-understand manner—more about breast cancer and prevention from a credible source. Both groups were favorable toward novel educational tools, such as the mobile web app education, and cited potential health benefits, particularly for women aged 40s to 60s﻿. Our findings highlighted the importance of creating effective, culturally tailored educational interventions built into programs specific to AIs to increase understanding about breast cancer screening and promote screening behaviors among AI women. Particular attention to how AIs’ culture, beliefs, and barriers are implicated in screening behaviors could help with developing culturally tailored health education tools for this population.
Against methodological continuity and metaphysical knowledge
Authors: Allzén Simon 
Published: January 2023

AbstractThe main purpose of this paper is to refute the ‘methodological continuity’ argument supporting epistemic realism in metaphysics. This argument aims to show that scientific realists have to accept that metaphysics is as rationally justified as science given that they both employ inference to the best explanation, i.e. that metaphysics and science are methodologically continuous. I argue that the reasons given by scientific realists as to why inference to the best explanation (IBE) is reliable in science do not constitute a reason to believe that it is reliable in metaphysics. The justification of IBE in science and the justification of IBE in metaphysics are two distinct issues with only superficial similarities, and one cannot rely on one for the other. This becomes especially clear when one analyses the debate about the legitimacy of IBE that has taken place between realists and empiricists. The metaphysician seeking to piggyback on the realist defense of IBE in science by the methodological continuity argument presupposes that the defense is straightforwardly applicable to metaphysics. I will argue that it is, in fact, not. The favored defenses of IBE by scientific realists make extensive use of empirical considerations, predictive power and inductive evidence, all of which are paradigmatically absent in the metaphysical context. Furthermore, even if the realist would concede the methodological continuity argument, I argue that the metaphysician fails to offer any agreed upon conclusions resulting from its application in metaphysics.
A comprehensive method for determining the dewaxing interval period in gas lift wells
Authors: Nguyen Van Thang , Pham Thanh Vinh , Rogachev Mikhail Konstantinovich , Korobov Grigory Yurievich , Parfenov Dmitry Viktorovich , Zhurkevich Aleksei Olegovich , Islamov Shamil Rasikhovich 
Published: January 2023

AbstractThe gas lift method is an artificial lift method of well production using the energy of compressed gas injected into the well to lift the reservoir fluid to the surface. The formation of paraffin deposits has remained one of the critical oil production problems due to the growing percentage of hard-to-recover reserves in the overall structure. This complication is also typical for many oil and gas fields in Vietnam, such as the White Tiger, White Bear, and Dragon fields. Wax deposit formation negatively affects the operation of individual production wells and the development of the field as a whole, which leads to a decrease in productivity and the need to take measures to remove paraffin deposits, subsequently increasing the downtime period of the well. In order to ensure stable production of highly paraffinic oil, it is necessary to take measures to dewax wells systematically. The frequency of wax removal operations depends on the intensity of the wax formation, which is determined by various technological, technical, and geological factors. The interval between dewaxing operations is called the dewaxing interval period (DIP). This value is an important technological parameter and characterizes the efficiency of a well’s operation. In this study, a comprehensive method has been developed to determine the dewaxing interval period (treatment interval) for gas-lift wells when the formation of wax deposits has occurred. The optimal dewaxing interval period is suggested to be determined by the change in the liquid well flow rate at the point when it falls by 20% from the initial value. In addition, a mathematical model of the time-dependent wax thickness, taking into account the heat and mass transfer laws and the laboratory results using the Cold Finger method, has also been developed. The proposed model for determining the dewaxing interval period was applied to an oil well in Vietnam. The DIP prediction model gave a similar value to the actual DIP field data (6.67 and 6 days, respectively). The obtained results showed that the model had proven its accuracy following the results of a comparison with the field's data of dewaxing operations.
Inulin-lipid hybrid (ILH) microparticles promote pH-triggered release of rifampicin within infected macrophages
Authors: Maghrebi Sajedeh , Thomas Nicky , Prestidge Clive A. , Joyce Paul 
Published: January 2023

AbstractIntracellular bacteria serve as a problematic source of infection due to their ability to evade biological immune responses and the inability for conventional antibiotics to efficiently penetrate cellular membranes. Subsequently, new treatment approaches are urgently required to effectively eradicate intracellular pathogens residing within immune cells (e.g. macrophages). In this study, the poorly soluble and poorly permeable antibiotic, rifampicin, was re-purposed via micro-encapsulation within inulin-lipid hybrid (ILH) particles for the treatment of macrophages infected with small colony variants of Staphylococcus aureus (SCV S. aureus). Rifampicin-encapsulated ILH (Rif-ILH) microparticles were synthesized by spray drying a lipid nano-emulsion, with inulin dissolved throughout the aqueous phase and rifampicin pre-loaded within the lipid phase. Rif-ILH were strategically designed and engineered with pH-responsive properties to promote lysosomal drug release upon cellular internalization, while preventing premature rifampicin release in plasma-simulating media. The pH-responsiveness of Rif-ILH was controlled by the acid-mediated hydrolysis of the inulin coating, where exposure to acidic media simulating the lysosomal environment of macrophages triggered hydrolysis of the oligofructose chain and the subsequent diffusion of rifampicin from Rif-ILH. This pH-provoked release mechanism, as well as the ability for ILH microparticles to be more readily internalized by macrophages, was found to be influential in triggering a 2.9-fold increase in intracellular rifampicin concentration within infected macrophages, compared to the pure drug. The subsequent increase in exposure of intracellular pathogens to rifampicin leads to a ~ 2-log improvement in antibacterial activity for Rif-ILH, at a rifampicin dose of 2.5 µg/mL. Thus, the reduction in viability of intracellular SCV S. aureus, in the absence of cellular toxicity, is indicative of ILH microparticles serving as a unique approach for the safe and efficacious delivery of antibiotics to phagocytic cells for the treatment of intracellular infections.
Graphical Abstract
SPOCK2 and SPRED1 function downstream of EZH2 to impede the malignant progression of lung adenocarcinoma in vitro and in vivo
Authors: Liu Yang , Fan Xiaoxi , Jiang Changrui , Xu Shun 
Published: January 2023

AbstractEnhancer of zeste homolog 2 (EZH2) is an important epigenetic regulator, and is associated with the malignant progression of lung cancer. However, the mechanisms of EZH2 on lung adenocarcinoma (LUAD) remain unclear. The relationship between EZH2 and SPOCK2 or SPRED1 was confirmed by dual-luciferase reporter assay. The Cancer Genome Atlas (TCGA) and Gene Expression Omnibus (GEO) databases were analyzed to examine the expression of SPOCK2 and SPRED1 and their prognostic values of LUAD. The effects of SPOCK2 and SPRED1 on the biological characters of LUAD cells were identified on functional assays in vitro and in vivo. Our results showed that EZH2 suppressed the expression and transcriptional activity of SPOCK2 and SPRED1, and these effects were reversed by the EZH2 inhibitor, Tazemetostat. SPOCK2 and SPRED1 were expressed at low levels in LUAD patients, and a high expression level of SPOCK2 or SPRED1 predicted better survival. Moreover, overexpression of SPOCK2 or SPRED1 could inhibit tumoral proliferation, migration ratio, and invasion activity in vitro as well as retard tumor growth in vivo. However, EZH2 elevation could rescue these impacts and accelerate LUAD progression. Our findings reveal that SPOCK2 and SPRED1 are epigenetically suppressed by EZH2 and may act as novel regulators to inhibit the proliferation, migration, and invasion of LUAD cells.
Degenerate Kolmogorov equations and ergodicity for the stochastic Allen–Cahn equation with logarithmic potential
Authors: Scarpa Luca , Zanella Margherita 
Published: January 2023

AbstractWell-posedness à la Friedrichs is proved for a class of degenerate Kolmogorov equations associated to stochastic Allen–Cahn equations with logarithmic potential. The thermodynamical consistency of the model requires the potential to be singular and the multiplicative noise coefficient to vanish at the respective potential barriers, making thus the corresponding Kolmogorov equation not uniformly elliptic in space. First, existence and uniqueness of invariant measures and ergodicity are discussed. Then, classical solutions to some regularised Kolmogorov equations are explicitly constructed. Eventually, a sharp analysis of the blow-up rates of the regularised solutions and a passage to the limit with a specific scaling yield existence à la Friedrichs for the original Kolmogorov equation.
Diagnostic capabilities of transperineal ultrasound (TPUS) to evaluate anal sphincter defect post obstetric anal sphincter injury (OASIS)? A systematic review
Authors: Hakim Surahman , Santoso Budi Iman , Djusad Suskhan , Moegni Fernandi , Surya Raymond , Kurniawan Andrew Pratama 
Published: January 2023

Abstract
Introduction
Endoanal ultrasound (3D-EAUS) is the gold standard imaging investigation for evaluating the anal sphincter; unfortunately, it is not universally available in most obstetric units. This study aims to appraise the ability of transperineal ultrasound (TPUS) compared with 3D-EAUS as the gold standard to identify anal sphincter defects after primary repair of OASIS.

Methods
A systematic search of major databases to identify diagnostic accuracy of 3D-TPUS in evaluating anal sphincter defects. Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines were designed for this systematic review. The risk of bias and applicability concerns were assessed using the QUADAS-2 tool. Our eligibility criteria are patients with a history of primary repair of anal sphincter injuries (OASIS). They were followed up after the primary repair to detect the anal sphincter defect using 3D-TPUS vs. 3D-EAUS as a gold standard.

Results
Two eligible observational studies were included and assessed for risk of bias using the QUADAS-2 tool and showed a low risk of bias and a low risk of concerns. 3D-TPUS had various sensitivity to detect external anal sphincter defects in two studies; meanwhile, the specificity was around 67–70%. For detecting the internal anal sphincter defects, 3D-TPUS had low sensitivity but high specificity (93–94%).

Conclusion
3D-TPUS had various sensitivity to detect external anal sphincter defects and low sensitivity to detect internal anal sphincter defects. On the other hand, 3D-TPUS had low specificity for detecting external anal sphincter defects and high specificity for detecting internal anal sphincter defects.
There Is More to Wounds than Bacteria: Fungal Biofilms in Chronic Wounds
Authors: Short Bryn , Bakri Ahmed , Baz Abdullah , Williams Craig , Brown Jason , Ramage Gordon 
Published: January 2023

AbstractPurpose of ReviewThe management of chronic wounds, a debilitating condition, presents a considerable challenge to healthcare professionals and a significant burden on services. When these wounds are exposed to the external environment, they are susceptible to microbial infection, which further complicates their management and worsens clinical outcomes.Recent FindingsBacteria typically exist in wounds as part of a biofilm, which is often polymicrobial in nature, alongside bacteria and fungi that are described as being more virulent and tolerant towards antimicrobials and antiseptics. Despite advancing knowledge in polymicrobial biofilm wound infections with respect to bacteria, the role of fungi is largely ignored, and their influence in chronicity and clinical management is not fully appreciated or understood.SummaryThe purpose of this review is to explore the significance of fungi within chronic wound environments and, in doing so, understand the importance of interkingdom interactions in wound management.
Harmonic structure of wave loads on a surface piercing column in directionally spread and unidirectional random seas
Authors: Mj Dripta , McAllister Mark L. , Bredmose Henrik , Adcock Thomas A. A. , Taylor Paul H. 
Published: January 2023

AbstractWe investigate the harmonic structure of wave-induced loads on vertical cylinders with dimensions comparable to those used to support offshore wind turbines. Many offshore wind turbines are designed, so the structural resonance is two or three times the fundamental wave loading period, which may be excited by higher harmonics of wave loading. The suitability of a ‘Stokes-type’ model for force is examined. We analyse experimental data for unidirectional and directionally spread sea-states. We demonstrate that approximate harmonic components may be extracted from a random time series, using a novel signal processing method. The extracted harmonics are shown to follow a ‘Stokes-like’ model, where the higher harmonics in frequency are proportional to the n-th power of the linear inline force component and its Hilbert transform. Results show that the third harmonic component of force is fitted less well by this model, which is consistent with the literature. A key new result is that the harmonic coefficients for spread and unidirectional seas are nearly identical when fitted as powers of the linear inline force and its Hilbert transform. This finding has the potential to greatly simplify the process of generating harmonic data for new wavelength to cylinder and wavelength to depth ratios. This also implies that the size of the inline component of the $$n\textrm{th}$$

n
th

harmonic for force in a directional sea relative to the same component in a unidirectional sea scales as $$\cos ^n \sigma _\theta $$

cos
n

σ
θ

, where $$\sigma _\theta $$

σ
θ

is the rms directional spreading angle of the incident wave field. Hence, higher harmonics will be of less importance in directionally spread seas than unidirectional ones. We show that the computationally fast harmonic decomposition approach taken here can reproduce the shape and magnitude of the loading from non-breaking waves waves in a wide range of realistic unidirectional and directionally spread sea-states. The proposed force model has potential as an engineering tool for computationally fast prediction of nonlinear wave loads.
Feasibility of Group-Based Implementation Facilitation for Video Telemental Health
Authors: Ecker Anthony H. , Day Giselle , Amspoker Amber B. , Bryan Jennifer L. , Day Stephanie C. , Wassef Miryam , Weaver Kendra , Lindsay Jan 
Published: January 2023

Abstract
Video telehealth experienced rapid growth throughout the COVID-19 pandemic in many healthcare sectors, including mental health. The Veterans Health Administration’s video telehealth platform, VA Video Connect, has been widely used to reach veterans who may have experienced difficulty accessing care, such as those living in rural areas or other barriers (e.g., transportation). Implementing VVC requires a multifaceted approach, including training providers on technical skills, increasing access to equipment for providers and veterans, and integrating VVC within the culture and processes of the clinic unit. Prior successful VVC implementation efforts in rural areas have focused on simultaneous one-on-one provider and leadership engagement using implementation facilitation (IF). However, given the rapid need for VVC expansion in light of limits and dangers associated with in-person care during the pandemic, our team developed group facilitation to increase the reach of VVC implementation through IF. Group facilitation combined training in technical and policy elements of VVC with IF with groups of providers from clinic units. This approach was designed to rapidly disseminate the necessary knowledge to conduct VVC combined with collaborative problem solving as a team to improve the ability of the clinical team to sustain VVC. Attendees were asked for feedback on the session through multiple choice and open-ended questions. Participants (N = 26) reported being highly satisfied with the training and reported a high degree of confidence in their ability to use VVC. Based on evaluation data and interview feedback, providers and clinic leaders were satisfied with group facilitation. Group facilitation may be a helpful tool in rapidly training clinical teams to implement and sustain video telemental health.
The MING proposal at SHINE: megahertz cavity enhanced X-ray generation
Authors: Huang Nan-Shun , Liu Zi-Peng , Deng Bang-Jie , Zhu Zi-Han , Li Shao-Hua , Liu Tao , Qi Zheng , Yan Jia-Wei , Zhang Wei , Xiang Sheng-Wang , Lei Yang-Yang , Zhu Ya , He Yong-Zhou , Yuan Qi-Bing , Gao Fei , Deng Rong-Bing , Sun Sen , Lei Zhi-Di , Jiang Zhi-Qiang , Duan Meng-Qi , Zhuan Yuan , Huang Xue-Fang , Dong Peng-Cheng , Li Zhong-Liang , Si Shang-Yu , Xue Lian , Chen Si , Liu Yong-Fang , Tong Ya-Jun , Deng Hai-Xiao , Zhao Zhen-Tang 
Published: January 2023

AbstractThe cavity-based X-ray free-electron laser (XFEL) has promise in producing fully coherent pulses with a bandwidth of a few meV and very stable intensity, whereas the currently existing self-amplified spontaneous emission (SASE) XFEL is capable of generating ultra-short pulses with chaotic spectra. In general, a cavity-based XFEL can provide a spectral brightness three orders of magnitude higher than that of the SASE mode, thereby opening a new door for cutting-edge scientific research. With the development of superconducting MHz repetition-rate XFEL facilities such as FLASH, European-XFEL, LCLS-II, and SHINE, practical cavity-based XFEL operations are becoming increasingly achievable. In this study, megahertz cavity enhanced X-ray generation (MING) is proposed based on China’s first hard XFEL facility - SHINE, which we refer to as MING@SHINE.