bluuebunny/crossref_metadata_2025_split huggingface dataset:
The pitch contour of the French discourse marker donc: A corpus-based study using generalized additive mixed modeling
Authors: Deng Delin , Wang Fenqi , Wayland Ratree 
Published: June 2023

AbstractIn this article, we explored the pitch contour patterns of the French discourse marker donc in realizing different pragmatic functions from native and non-native oral corpora in French. Statistical analyses using generalized additive mixed modeling revealed that even though Mandarin Chinese L1 speakers learning French also used the pitch cue to realize pragmatic functions, their prosodic pattern is different from the native pattern. Their L1 Chinese seemed to influence their usage of the pitch cue significantly. In addition, women were shown to be better than men in using the pitch cue in conveying pragmatic functions with a closer pattern to the native pattern. Overall, our study sheds new light on the relationship between speakers’ L1 and L2 regarding the interaction between pragmatic and prosodic features. It also provides new reflections on the acquisition of socio-pragmatic competence.
Integrity and Moral Flexibility
Authors: Hart William , Cease Charlotte K. , Lambert Joshua T. , Witt Danielle E. 
Published: June 2023

Abstract: People with high integrity should stubbornly insist on maintaining their moral stances regardless of how their stances will make them appear. However, we examined whether people who claim to be high in integrity will express different moral stances to appear high in integrity. Participants ( N = 433) self-reported their integrity and then read and responded to hypothetical moral dilemmas that introduced tension between utilitarian and deontological moral principles. Participants reported their willingness to choose the utilitarian (vs. deontological) option in each dilemma under conditions in which choosing the utilitarian option signaled high integrity (utilitarian-signals-high-integrity condition) or choosing the deontological option signaled high integrity (deontological-signals-high-integrity condition). Generally, participants reported greater willingness to choose the utilitarian (vs. deontological) option in the utilitarian-signals-high-integrity (vs. deontological-signals-high-integrity) condition, but this effect was greater amongst people higher in self-proclaimed integrity. Additional analyses produced similar effects after accounting for participant sex, other moral personality traits, and experimenter demand. Broadly, the findings support the possibility that self-proclaimed integrity may be partly based on a self-presentation process.
Peli3 ablation ameliorates acetaminophen-induced liver injury through inhibition of GSK3β phosphorylation and mitochondrial translocation
Authors: Lee Jaewon , Ha Jihoon , Kim Jun-Hyeong , Seo Dongyeob , Kim Minbeom , Lee Yerin , Park Seong Shil , Choi Dahee , Park Jin Seok , Lee Young Jae , Yang Siyoung , Yang Kyung-Min , Jung Su Myung , Hong Suntaek , Koo Seung-Hoi , Bae Yong-Soo , Kim Seong-Jin , Park Seok Hee 
Published: June 2023

AbstractThe signaling pathways governing acetaminophen (APAP)-induced liver injury have been extensively studied. However, little is known about the ubiquitin-modifying enzymes needed for the regulation of APAP-induced liver injury. Here, we examined whether the Pellino3 protein, which has E3 ligase activity, is needed for APAP-induced liver injury and subsequently explored its molecular mechanism. Whole-body Peli3−/− knockout (KO) and adenovirus-mediated Peli3 knockdown (KD) mice showed reduced levels of centrilobular cell death, infiltration of immune cells, and biomarkers of liver injury, such as alanine aminotransferase (ALT) and aspartate aminotransferase (AST), upon APAP treatment compared to wild-type (WT) mice. Peli3 deficiency in primary hepatocytes decreased mitochondrial and lysosomal damage and reduced the mitochondrial reactive oxygen species (ROS) levels. In addition, the levels of phosphorylation at serine 9 in the cytoplasm and mitochondrial translocation of GSK3β were decreased in primary hepatocytes obtained from Peli3−/− KO mice, and these reductions were accompanied by decreases in JNK phosphorylation and mitochondrial translocation. Pellino3 bound more strongly to GSK3β compared with JNK1 and JNK2 and induced the lysine 63 (K63)-mediated polyubiquitination of GSK3β. In rescue experiments, the ectopic expression of wild-type Pellino3 in Peli3−/− KO hepatocytes restored the mitochondrial translocation of GSK3β, but this restoration was not obtained with expression of a catalytically inactive mutant of Pellino3. These findings are the first to suggest a mechanistic link between Pellino3 and APAP-induced liver injury through the modulation of GSK3β polyubiquitination.
Photonic structures in radiative cooling
Authors: Lee Minjae , Kim Gwansik , Jung Yeongju , Pyun Kyung Rok , Lee Jinwoo , Kim Byung-Wook , Ko Seung Hwan 
Published: June 2023

AbstractRadiative cooling is a passive cooling technology without any energy consumption, compared to conventional cooling technologies that require power sources and dump waste heat into the surroundings. For decades, many radiative cooling studies have been introduced but its applications are mostly restricted to nighttime use only. Recently, the emergence of photonic technologies to achieves daytime radiative cooling overcome the performance limitations. For example, broadband and selective emissions in mid-IR and high reflectance in the solar spectral range have already been demonstrated. This review article discusses the fundamentals of thermodynamic heat transfer that motivates radiative cooling. Several photonic structures such as multilayer, periodical, random; derived from nature, and associated design procedures were thoroughly discussed. Photonic integration with new functionality significantly enhances the efficiency of radiative cooling technologies such as colored, transparent, and switchable radiative cooling applications has been developed. The commercial applications such as reducing cooling loads in vehicles, increasing the power generation of solar cells, generating electricity, saving water, and personal thermal regulation are also summarized. Lastly, perspectives on radiative cooling and emerging issues with potential solution strategies are discussed.
Characterisation of cell lines derived from prostate cancer patients with localised disease
Authors: Moya Leire , Walpole Carina , Rae Fiona , Srinivasan Srilakshmi , Seim Inge , Lai John , Nicol David , Williams Elizabeth D. , Clements Judith A. , Batra Jyotsna 
Published: June 2023

Abstract
Background
Prostate cancer is a broad-spectrum disease, spanning from indolent to a highly aggressive lethal malignancy. Prostate cancer cell lines are essential tools to understanding the basic features of this malignancy, as well as in identifying novel therapeutic strategies. However, most cell lines routinely used in prostate cancer research are derived from metastatic disease and may not fully elucidate the molecular events underlying the early stages of cancer development and progression. Thus, there is a need for new cell lines derived from localised disease to better span the disease spectrum.

Methods
Prostatic tissue from the primary site, and adjacent non-cancerous tissue was obtained from four patients with localised disease undergoing radical prostatectomy. Epithelial cell outgrowths were immortalised with human papillomavirus type 16 (HPV16) E6 and E7 to establish monoclonal cell lines. Chromosomal ploidy was imaged and STR profiles were determined. Cell morphology, colony formation and cell proliferation characteristics were assessed. Androgen receptor (AR) expression and AR-responsiveness to androgen treatment were analysed by immunofluorescence and RT-qPCR, respectively. RNA-seq analysis was performed to identify prostate lineage markers and expression of prostate cancer tumorigenesis-related genes.

Results
Two benign cell lines derived from non-cancer cells (AQ0420 and AQ0396) and two tumour tissue derived cancer cell lines (AQ0411 and AQ0415) were immortalised from four patients with localised prostatic adenocarcinoma. The cell lines presented an epithelial morphology and a slow to moderate proliferative rate. None of the cell lines formed anchorage independent colonies or displayed AR-responsiveness. Comparative RNA-seq expression analysis confirmed the prostatic lineage of the four cell lines, with a distinct gene expression profile from that of the metastatic prostate cancer cell lines, PC-3 and LNCaP.

Conclusions
Comprehensive characterization of these cell lines may provide new in vitro tools that could bridge the current knowledge gap between benign, early-stage and metastatic disease.
Phyllosphere symbiont promotes plant growth through ACC deaminase production
Authors: Herpell Johannes B , Alickovic Ajtena , Diallo Bocar , Schindler Florian , Weckwerth Wolfram 
Published: June 2023

Abstract
Plant growth promoting bacteria can confer resistance to various types of stress and increase agricultural yields. The mechanisms they employ are diverse. One of the most important genes associated with the increase in plant biomass and stress resistance is acdS, which encodes a 1-aminocyclopropane-1-carboxylate- or ACC-deaminase. The non-proteinogenic amino acid ACC is the precursor and means of long-distance transport of ethylene, a plant hormone associated with growth arrest. Expression of acdS reduces stress induced ethylene levels and the enzyme is abundant in rhizosphere colonizers. Whether ACC hydrolysis plays a role in the phyllosphere, both as assembly cue and in growth promotion, remains unclear. Here we show that Paraburkholderia dioscoreae Msb3, a yam phyllosphere symbiont, colonizes the tomato phyllosphere and promotes plant growth by action of its ACC deaminase. We found that acdS is required for improved plant growth but not for efficient leaf colonization. Strain Msb3 readily proliferates on the leaf surface of tomato, only occasionally spreading to the leaf endosphere through stomata. The strain can also colonize the soil or medium around the roots but only spreads into the root if the plant is wounded. Our results indicate that the degradation of ACC is not just an important trait of plant growth promoting rhizobacteria but also one of leaf dwelling phyllosphere bacteria. Manipulation of the leaf microbiota by means of spray inoculation may be more easily achieved than that of the soil. Therefore, the application of ACC deaminase containing bacteria to the phyllosphere may be a promising strategy to increasing plant stress resistance, pathogen control, and harvest yields.
Iontronic pressure sensor with high sensitivity over ultra-broad linear range enabled by laser-induced gradient micro-pyramids
Authors: Yang Ruoxi , Dutta Ankan , Li Bowen , Tiwari Naveen , Zhang Wanqing , Niu Zhenyuan , Gao Yuyan , Erdely Daniel , Xin Xin , Li Tiejun , Cheng Huanyu 
Published: June 2023

AbstractDespite the extensive developments of flexible capacitive pressure sensors, it is still elusive to simultaneously achieve excellent linearity over a broad pressure range, high sensitivity, and ultrahigh pressure resolution under large pressure preloads. Here, we present a programmable fabrication method for microstructures to integrate an ultrathin ionic layer. The resulting optimized sensor exhibits a sensitivity of 33.7 kPa−1 over a linear range of 1700 kPa, a detection limit of 0.36 Pa, and a pressure resolution of 0.00725% under the pressure of 2000 kPa. Taken together with rapid response/recovery and excellent repeatability, the sensor is applied to subtle pulse detection, interactive robotic hand, and ultrahigh-resolution smart weight scale/chair. The proposed fabrication approaches and design toolkit from this work can also be leveraged to easily tune the pressure sensor performance for varying target applications and open up opportunities to create other iontronic sensors.
Highly cross-linked carbon tube aerogels with enhanced elasticity and fatigue resistance
Authors: Zhuang Lei , Lu De , Zhang Jijun , Guo Pengfei , Su Lei , Qin Yuanbin , Zhang Peng , Xu Liang , Niu Min , Peng Kang , Wang Hongjie 
Published: June 2023

AbstractCarbon aerogels are elastic, mechanically robust and fatigue resistant and are known for their promising applications in the fields of soft robotics, pressure sensors etc. However, these aerogels are generally fragile and/or easily deformable, which limits their applications. Here, we report a synthesis strategy for fabricating highly compressible and fatigue-resistant aerogels by assembling interconnected carbon tubes. The carbon tube aerogels demonstrate near-zero Poisson’s ratio, exhibit a maximum strength over 20 MPa and a completely recoverable strain up to 99%. They show high fatigue resistance (less than 1.5% permanent degradation after 1000 cycles at 99% strain) and are thermally stable up to 2500 °C in an Ar atmosphere. Additionally, they possess tunable conductivity and electromagnetic shielding. The combined mechanical and multi-functional properties offer an attractive material for the use in harsh environments.
Ageing restructures the transcriptome of the hypothalamic supraoptic nucleus and alters the response to dehydration
Authors: Elsamad Ghadir , Mecawi André Souza , Pauža Audrys G. , Gillard Benjamin , Paterson Alex , Duque Victor J. , Šarenac Olivera , Žigon Nina Japundžić , Greenwood Mingkwan , Greenwood Michael P. , Murphy David 
Published: June 2023

AbstractAgeing is associated with altered neuroendocrine function. In the context of the hypothalamic supraoptic nucleus, which makes the antidiuretic hormone vasopressin, ageing alters acute responses to hyperosmotic cues, rendering the elderly more susceptible to dehydration. Chronically, vasopressin has been associated with numerous diseases of old age, including type 2 diabetes and metabolic syndrome. Bulk RNAseq transcriptome analysis has been used to catalogue the polyadenylated supraoptic nucleus transcriptomes of adult (3 months) and aged (18 months) rats in basal euhydrated and stimulated dehydrated conditions. Gene ontology and Weighted Correlation Network Analysis revealed that ageing is associated with alterations in the expression of extracellular matrix genes. Interestingly, whilst the transcriptomic response to dehydration is overall blunted in aged animals compared to adults, there is a specific enrichment of differentially expressed genes related to neurodegenerative processes in the aged cohort, suggesting that dehydration itself may provoke degenerative consequences in aged rats.
Biodegradable elastomeric circuit boards from citric acid-based polyesters
Authors: Turner Brendan L. , Twiddy Jack , Wilkins Michael D. , Ramesh Srivatsan , Kilgour Katie M. , Domingos Eleo , Nasrallah Olivia , Menegatti Stefano , Daniele Michael A. 
Published: June 2023

AbstractRecyclable and biodegradable microelectronics, i.e., “green” electronics, are emerging as a viable solution to the global challenge of electronic waste. Specifically, flexible circuit boards represent a prime target for materials development and increasing the utility of green electronics in biomedical applications. Circuit board substrates and packaging are good dielectrics, mechanically and thermally robust, and are compatible with microfabrication processes. Poly(octamethylene maleate (anhydride) citrate) (POMaC) – a citric acid-based elastomer with tunable degradation and mechanical properties – presents a promising alternative for circuit board substrates and packaging. Here, we report the characterization of Elastomeric Circuit Boards (ECBs). Synthesis and processing conditions were optimized to achieve desired degradation and mechanical properties for production of stretchable circuits. ECB traces were characterized and exhibited sheet resistance of 0.599 Ω cm−2, crosstalk distance of <0.6 mm, and exhibited stable 0% strain resistances after 1000 strain cycles to 20%. Fabrication of single layer and encapsulated ECBs was demonstrated.
Feedback-based active reset of a spin qubit in silicon
Authors: Kobayashi T. , Nakajima T. , Takeda K. , Noiri A. , Yoneda J. , Tarucha S. 
Published: June 2023

AbstractFeedback control of qubits is a highly demanded technique for advanced quantum information protocols such as fault-tolerant quantum error correction. Here we demonstrate active reset of a silicon spin qubit using feedback control. The active reset is based on quantum non-demolition (QND) readout of the qubit and feedback according to the readout results, which is enabled by hardware data processing and sequencing. We incorporate a cumulative readout technique to the active reset protocol, enhancing initialization fidelity above a limitation imposed by the single-shot QND readout fidelity. An analysis of the reset protocol implies a pathway to achieve the initialization fidelity sufficient for fault-tolerant quantum computation. These results provide a practical approach to high-fidelity qubit operations in realistic devices.
Transport measurement of fractional charges in topological models
Authors: Cheng Shu-guang , Wu Yijia , Jiang Hua , Sun Qing-Feng , Xie X. C. 
Published: June 2023

AbstractThe static topological fractional charge (TFC) in condensed matter systems is related to the band topology and thus has potential applications in topological quantum computation. However, the experimental measurement of these TFCs in electronic systems is quite challenging. We propose an electronic transport measurement scheme in which both the charge amount and the spatial distribution of the TFC can be extracted from the differential conductance through a quantum dot coupled to the topological system being measured. For one-dimensional Su–Schrieffer–Heeger (SSH) model, both the e/2 charge of the TFC and its distribution can be verified. As for the disorder effect, it is shown that the Anderson disorder, which breaks certain symmetry related to the TFC, is significant in higher-dimensional systems while having little effect on the one-dimensional SSH chain. Nonetheless, our measurement scheme can still work well for specific higher-order topological insulator materials, for instance, the 2e/3 TFC in the breathing kagome model could be confirmed even in the presence of disorder effect. These conclusions about spatial dimension and disorder effect are quite universal, which also applies to other topological systems such as topological classic wave system.
Evidence-based target setting informs blue carbon strategies for nationally determined contributions
Authors: Arkema Katie K. , Delevaux Jade M. S. , Silver Jessica M. , Winder Samantha G. , Schile-Beers Lisa M. , Bood Nadia , Crooks Stephen , Douthwaite Karen , Durham Courtney , Hawthorne Peter L. , Hickey Thomas , Mattis Colin , Rosado Andria , Ruckelshaus Mary , von Unger Moritz , Young Arlene 
Published: June 2023

AbstractThe magnitude and pace of global climate change demand ambitious and effective implementation of nationally determined contributions (NDCs). Nature-based solutions present an efficient approach to achieving mitigation, adaptation and resilience goals. Yet few nations have quantified the diverse benefits of nature-based solutions to evaluate and select ecosystem targets for their NDCs. Here we report on Belize’s pursuit of innovative, evidence-based target setting by accounting for multiple benefits of blue carbon strategies. Through quantification of carbon storage and sequestration and optimization of co-benefits, we explore time-bound targets and prioritize locations for mangrove protection and restoration. We find increases in carbon benefits with larger mangrove investments, while fisheries, tourism and coastal risk-reduction co-benefits grow initially and then plateau. We identify locations, currently lacking protected status, where prioritizing blue carbon strategies would provide the greatest delivery of co-benefits to communities. These findings informed Belize’s updated NDCs to include an additional 12,000 ha of mangrove protection and 4,000 ha of mangrove restoration, respectively, by 2030. Our study serves as an example for the more than 150 other countries that have the opportunity to enhance greenhouse gas sequestration and climate adaptation by incorporating blue carbon strategies that provide multiple societal benefits into their NDCs.
Leafhopper males compensate for unclear directional cues in vibration-mediated mate localization
Authors: Polajnar Jernej , Kuhelj Anka , Janža Rok , Žnidaršič Nada , Simčič Tatjana , Virant-Doberlet Meta 
Published: June 2023

AbstractAmbient noise and transmission properties of the substrate pose challenges in vibrational signal-mediated mating behavior of arthropods, because vibrational signal production is energetically demanding. We explored implications of these challenges in the leafhopper Aphrodes makarovi (Insecta: Hemiptera: Cicadellidae) by exposing males to various kinds of vibrational noise on a natural substrate and challenging them to find the source of the female playback. Contrary to expectations, males exposed to noise were at least as efficient as control males on account of similar searching success with less signaling effort, while playing back male–female duets allowed the males to switch to satellite behavior and locate the target without signaling, as expected. We found altered mitochondrial structure in males with high signaling effort that likely indicate early damaging processes at the cellular level in tymbal muscle, but no relation between biochemical markers of oxidative stress and signaling effort. Analysis of signal transmission revealed ambiguous amplitude gradients, which might explain relatively low searching success, but it also indicates the existence of behavioral adaptations to complex vibrational environments. We conclude that the observed searching tactic, emphasizing speed rather than thorough evaluation of directional cues, may compensate for unclear stimuli when the target is near.
Gut microbiota-derived lipid metabolites facilitate regulatory T cell differentiation
Authors: Shiratori Hiroaki , Oguchi Hiroyuki , Isobe Yosuke , Han Kyu-Ho , Sen Akira , Yakebe Kyosuke , Takahashi Daisuke , Fukushima Michihiro , Arita Makoto , Hase Koji 
Published: June 2023

AbstractCommensal bacteria-derived metabolites are critical in regulating the host immune system. Although the impact of gut microbiota-derived hydrophilic metabolites, such as short-chain fatty acids, on immune cell functions and development has been well documented, the immunomodulatory effects of gut microbiota-derived lipids are still of interest. Here, we report that lipid extracts from the feces of specific-pathogen-free (SPF), but not germ-free (GF), mice showed regulatory T (Treg)-cell-inducing activity. We conducted RP-HPLC-based fractionation and liquid chromatography–tandem mass spectrometry (LC–MS/MS)-based lipidome profiling and identified two bioactive lipids, 9,10-dihydroxy-12Z-octadecenoic acid (9,10-DiHOME) and all-trans retinoic acid (atRA), with Treg-inducing activity in vitro. The luminal abundance of 9,10-DiHOME in the large intestine was significantly decreased by dextran sulfate sodium (DSS)-induced colitis, indicating that 9,10-DiHOME may be a potential biomarker of colitis. These observations implied that commensal bacteria-derived lipophilic metabolites might contribute to Treg development in the large intestine.