bluuebunny/crossref_metadata_2025_split huggingface dataset:
Overlooked and misunderstood: A morpho‐molecular revision of Ditrichaceae s.str. (Dicranidae, Bryophyta), with a focus on the Holarctic species of Ditrichum
Authors: Fedosov Vladimir , Fedorova Alina , Ignatova Elena , Brinda John C. , Kučera Jan 
Published: January 2025

AbstractWe revised the Holarctic taxa of Ditrichaceae on the basis of both organellar DNA sequence data and morphological traits. After the removal of phylogenetically discordant elements related to Flexitrichum and Rhamphidium, the remaining representatives of the family cluster into three well‐supported lineages, which are recognized here as the reinstated family Ceratodontaceae and a more restricted Ditrichaceae with two subfamilies. Rhamphidium is segregated into a newly described family, Rhamphidiaceae; Aongstroemia gayana, Dicranella vaginata and Symblepharis krausei are combined under Rhamphidium, and the genus Strombulidens is tentatively moved to Rhamphidiaceae. Ceratodontaceae includes the genera Ceratodon, Cheilothela, Trichodon, and a newly described Pseudaongstroemia, which includes a single species, known earlier as Aongstroemia fuji‐alpina. The genus Trichodon is paraphyletically defined to include T. cylindricus, T. oblongus reinstated at specific rank, and a molecularly distinct lineage, which we were unable to distinguish morphologically from T. cylindricus. The monoicous genera of Ditrichaceae and the autoicous taxa of Ditrichum as previously understood are merged into a single genus, Pleuridium, which is segregated to Ditrichaceae subfam. Pleuridioideae stat. nov. A single genus is also accepted within Ditrichaceae subfam. Ditrichoideae, following the transfer of both Aongstroemia julacea s.str. and A. orientalis to Ditrichum. Ditrichum septentrionale is newly described based on material mostly from North Asia. Two ecologically specialized, putatively only locally distributed taxa are synonymized with more widespread taxa. Ditrichum plumbicola is placed into synonymy with D. lineare and similarly D. cornubicum is tentatively synonymized with D. macrorhynchum. The morphological delimitations of D. lineare, D. pusillum and D. macrorhynchum are clarified and emended.
Developing Virtual Classroom Tours With Preservice Teachers: Integrating a Translanguaging Stance and Design
Authors: Seltzer Kate 
Published: January 2025

ABSTRACTThis article describes a project for a university course on teaching language and literacy through a translanguaging lens. Through this project, preservice teachers (PSTs) were invited to design a “Virtual Classroom Tour” for a new multilingual learner (ML). The resulting multilingual, multimodal classroom tours became reflections of PSTs' emerging translanguaging stances and enabled them make important connections between their beliefs and their future pedagogical decisions and practices.
Intellectual Property and Islamic Finance: Opportunities and Challenges for a New Islamic Intellectual Property Finance Framework
Authors: Naim Nadia , Kasri Noor Suhaida 
Published: January 2025

ABSTRACTThis paper explores the intersection of Intellectual Property (IP) and Islamic finance, proposing a policy framework to integrate IP valuation into Sharia‐compliant financing. Focusing on the UK, it also extends to global markets, including ASEAN, GCC, and MENA regions. The paper identifies gaps in current practices, particularly in incorporating IP assets into Islamic financial products, highlighting untapped market potential. It emphasizes the need for regulatory compliance, Sharia board approvals, and robust audit mechanisms to ensure ethical integration. Key recommendations include developing a comprehensive IP framework, fostering international collaboration, and capacity building through education. The framework aims to align economic growth with ethical financing, enhancing transparency and the robustness of Islamic IP financing globally.
Engineering of hierarchical nano‐convex polymer lattice SERS platforms by template replica‐assisted nanoimprinting
Authors: Dar Gohar Ijaz , Xifre‐Perez Elisabet , Santos Abel , Marsal Lluis F. 
Published: January 2025

AbstractThe precise fabrication of arrays of gold and silver nanodots (Au&Ag_NDs) to create plasmonic lattices on different substrates through cost‐effective, scalable approaches remains challenging. The aim of this research is to engineer a new class of hierarchical plasmonic lattices based on Au&Ag_NDs generated on polymer substrates via template replica‐assisted nanoimprinting. This method harnesses a combination of nanopatterning via anodization, magnetron sputtering, and annealing to generate Au&Ag_NDs hierarchical plasmonic lattices with tunable features at the nanoscale. These plasmonic lattices are explored as ultrasensitive surface‐enhanced Raman scattering (SERS) platforms for the detection of a model organic (rhodamine B [RhB]). These reproducible model platforms achieve remarkable enhancement to detect RhB molecules, with a low limit of detection of 10−8 M. The enhancement field factor of 107 indicates that the developed plasmonic lattices are adequate for efficient and highly sensitive SERS detection.
Diversity of Parasitic Fauna in Semi‐Scavenging Indigenous Chickens (Gallus gallus domesticus) in Bangladesh
Authors: Kausar‐A‐Noor  , Hasan Md. Mehadi , Anisuzzaman  , Alam Mohammad Zahangir , Khatun Mst. Sawda , Dey Anita Rani 
Published: January 2025

ABSTRACTBackgroundParasites are a major concern for profitable poultry production worldwide as they impede the health, welfare and production performance of poultry.ObjectivesThe present study was designed to detect the diversity of parasitic fauna and associated factors of gastrointestinal (GI) helminths and lice in indigenous chickens.MethodsA total of 310 indigenous chickens were collected from different villages at Gauripur and Mymensingh Sadar, Mymensingh, and Bangladesh, and various parasites were identified.ResultsOut of 310 indigenous semi‐scavenging chickens, 281 were infected with one or more species of helminths with an overall prevalence of 90.6%. The identified species of helminths were Ascaridia galli (60.6%), Heterakis gallinarum (29.0%) and Cheilospirura hamulosa (14.2%), Catatropis verrucosa (7.7%), Echinostoma revolutum (7.4%), Raillietina spp. (76.5%) and Hymenolepis spp. (5.8%). The prevalence of lice infestations was 74.2%, and identified species were Menopon gallinae (72.6%), Goniodes gigas (11.6%) and Lipeurus caponis (10.3%). Co‐infections with helminths were 65.8% and with lice were 19.4% in chickens. Univariate analysis was performed to measure the association between predictor variables and parasitic infections by considering several biotic and abiotic variables, including age, sex, flock size, farming nature, use of anthelmintic/insecticides and socio‐economic status of owners. No significant (p < 0.05) variation was found in helminth infections but large flock size (87.0%) and mixed farming nature (81.2%) were significantly associated with lice infestations in chickens.ConclusionsAwareness related to the management system of chickens rearing need to be increased for formulating control strategy against parasitic infections in indigenous chickens in Bangladesh.
Durchführungsrealität der Komplexmethode zur Todeszeiteingrenzung an deutschen rechtsmedizinischen Instituten
Authors: Anders-Lohner Sven , Khostwal Neda , Ondruschka Benjamin , Schröder Ann Sophie 
Published: January 2025

Zusammenfassung

Hintergrund
Die Komplexmethode gilt als Goldstandard für die Eingrenzung des Todeszeitintervalls. Hinsichtlich der Durchführungsrealität bezüglich der Nutzung der Teilmethoden liegen keine Daten vor. Ziel der Arbeit ist es, die Nutzung der Teilmethoden in der Praxis darzustellen.

Material und Methoden
Es erfolgte eine fragebogenbasierte Erhebung unter den deutschen rechtsmedizinischen Instituten (n = 32).

Ergebnisse und Diskussion
Der Fragebogenrücklauf betrug 87,5 %. Während die temperaturbasierten Teilmethoden und die Beurteilung früh postmortaler Phänomene regelhaft durchgeführt werden, zeigt sich ein sehr heterogenes Bild für die Nutzung supravitaler Reaktionen für die Todeszeiteingrenzung. Somit bleibt die Durchführungsrealität hinter der Lehr- und Handbuchrealität sowie der Leitlinie der DGRM (Deutsche Gesellschaft für Rechtsmedizin) zurück. Eine Konsensbildung und Harmonisierung wären wünschenswert, um eine Vergleichbarkeit der Ergebnisse in der Praxis zu gewährleisten.
Energy circulation associated with interannual waves in the tropical-subtropical Pacific
Authors: Wu Borui , Aiki Hidenori , Toyoda Takahiro , Ogata Tomomichi , Nagura Motoki 
Published: January 2025

Abstract
The present study has investigated interannual variations in the tropical-subtropical Pacific Ocean by applying Helmholtz decomposition to wave energy fluxes to indicate the direction of group velocity. The main source of energy is located in the central equatorial Pacific Ocean, while the anticyclonic and cyclonic circulation of wave energy appears in the western and eastern tropical Pacific Ocean, respectively. In the western basin, the anticyclonic circulation of wave energy is overridden at low and mid-latitude regions by the westward transfer of wave energy associated with the energy flux potential. Regional averages of the energy flux streamfunction and energy-flux potential associated with El Niño and La Niña events are similar to each other and show a quadratic dependence on the observed Niño-3 index. For example, the regional average of the energy-flux potential associated with the wind input in the central Pacific Ocean may be regressed as 0.72 ∆T
2 + 0.31 (in giga watts), where ∆T is the sea surface temperature anomaly (in degrees Celsius) associated with the Niño-3 index. The symmetry between El Niño and La Niña events is owing to the fact that the wave energy quantities do not differentiate between upwelling and downwelling (Kelvin) waves.
First year of in-house forensic neuropathology consultations in Helsinki, Finland
Authors: Oura Petteri , Mäkinen Hilla , Ruotsalainen Roosa , Ruokomäki Miko , Virtanen Antti , Hakkarainen Antti J. 
Published: January 2025

Abstract
In July 2023, an in-house forensic neuropathology consultation pilot was established at the Helsinki office of the Forensic Medicine Unit, Finnish Institute for Health and Welfare. This offered an alternative to the previous practice of full outsourcing to a hospital neuropathology department. This paper aims to introduce the first year experiences of the pilot. The in-house consultant team comprised two forensic pathologists with special training in neuropathology. In-house consultations were offered for medico-legal autopsy cases with traumatic brain injury (TBI) and hypoxic-ischaemic encephalopathy. The in-house histology laboratory participated in the pilot, implementing beta-amyloid precursor protein (β-APP) stain at the end of the pilot year. An electronic database was also developed for data collection and reporting. The characteristics, costs, and duration of the first 25 consultation cases were reviewed. The cases involved mostly male decedents (84.0%) with TBI (84.0%). The median total cost of a pilot case was €624, which was substantially lower compared to the previous outsourced practice (€1013 per case + €38/84 for each microscope slide with special/immunohistochemical stain, respectively). After the implementation of β-APP stain into the in-house laboratory service, the median total cost of a pilot case was reduced further to €94. The median duration of an in-house consultation was 2.3 months. Thanks to a favourable in-house atmosphere, the first year experiences are encouraging. While the costs of the in-house consultation practice appear to be markedly lower than those of an external provider, both alternatives should be available for cases where sufficient expertise cannot be found in-house.
Systematic optimisation of crude buccal swab lysate protocols for use with the ForenSeq™ DNA Signature Prep Kit
Authors: Martin Donna-Lee Pamela , Heathfield Laura Jane 
Published: January 2025

Abstract
The ForenSeq™ DNA Signature Prep kit has not been thoroughly tested with crude buccal swab lysates in large-scale population studies using massively parallel sequencing (MPS). Commonly used lysis buffers for swabs intending to undergo direct polymerase chain reaction (PCR) are SwabSolution™ and STR GO! Lysis Buffers, and these have been successfully used to generate population data using capillary electrophoresis (CE) systems. In this study, we investigated the performance and optimisation of SwabSolution™ and STR GO! lysates with the ForenSeq™ DNA Signature Prep workflow and addressed the challenge of failed MPS profiles in initial trials. To mitigate PCR inhibition in SwabSolution™ lysates, three optimisation methods were evaluated: dilution of lysates, addition of 5X AmpSolution® reagent, and purification with magnetic beads. For STR GO! lysates, we explored spin-column purification using the QIAamp® DNA Investigator kit, magnetic bead purification, and a pH adjustment with 1 M hydrochloric acid. Our findings indicated that the addition of 5X AmpSolution® was effective for overcoming PCR inhibition in SwabSolution™ lysates, thereby maintaining a direct PCR approach. Spin-column purification, however, is recommended for STR GO! lysates to minimise MPS profile failure rates. These improvements enhance first-time success rates of crude swab lysates, and reduce the need for repeat sampling and re-sequencing, making the workflow more suitable for large-scale population studies in forensic laboratories.

Graphical Abstract

Overview of the adaptations made to ensure a high-first time success rate with crude buccal swab lysates using the ForenSeq™ DNA Signature Prep kit workflow
The healing power of transcutaneous electrical nerve stimulation: a systematic review on its effects after breast surgery
Authors: Akutay Seda , Yüceler Kaçmaz Hatice , Ceyhan Özlem 
Published: January 2025

Abstract

Background
Transcutaneous electrical stimulation after breast cancer surgery has been utilized for various purposes, but the full efficacy of this treatment approach on postoperative symptoms remains unclear.

Aim
This study aimed to answer the question: Does transcutaneous electrical nerve stimulation significantly impact postoperative patient outcomes in individuals undergoing breast cancer surgery?

Methods
A systematic review of randomized controlled trials was conducted. Because of the limited number of studies included, it was not feasible to perform a meta-analysis. English-language publications from 2013 and 2024 that investigated the effects of transcutaneous electrical stimulation in breast cancer surgery patients were included. Electronic databases such as Web of Science, PubMed, Scopus, EBSCO, ScienceDirect, Cochrane Central Register of Controlled Trials, and Wiley Online Library were searched. Two independent investigators assessed the studies using the revised JBI risk of bias tool. Data from randomized trials were extracted by two researchers using the Cochrane data collection tool.

Results
Our comprehensive literature review identified 251 studies. After rigorous assessment, 12 articles met our inclusion criteria. Title and abstract screening excluded seven studies that did not involve surgery, used treatments other than TENS, included acupuncture, or did not measure pain outcomes. Among these, five studies involving 776 patients examined the effects of transcutaneous electrical stimulation on pain management in breast cancer surgery. In all of the studies reviewed, transcutaneous electrical stimulation had a beneficial effect on postoperative pain.

Conclusion
Transcutaneous electrical stimulation has significantly alleviated pain associated with breast cancer surgery. This therapeutic modality has improved patient satisfaction with analgesia by relieving pain; reducing analgesic use; reducing postoperative nausea and vomiting; increasing blood levels of IL-2, IFN-γ, and IL-2/IL-4 ratio; and reducing skin sensitivity. Transcutaneous electrical stimulation devices may improve postoperative patient outcomes and enhance the recovery process in people undergoing breast cancer surgery. The results of this study are limited by heterogeneity and the small number of included studies. Future research should prioritize standardization of intervention procedures and investigation of the long-term effects of TENS in postoperative care.

Registration
This study was registered in the PROSPERO registration system under the number CRD42024523558.
Rock strength and stress dependence of local flow-path connectivity within faults or fractures: a preliminary overview of virtual and in-situ hydraulic tests
Authors: Ishii Eiichi , Ozaki Yusuke , Aoyagi Kazuhei , Sugawara Kentaro 
Published: January 2025

Abstract
Global flow path connectivity along faults or fractures depends on the degree of local flow path connectivity within each fault or fracture and is a key control of groundwater flow and solute transport. However, the mechanical controls on spatial variations in local flow path connectivity within individual faults or fractures are poorly understood. Local flow path connectivity is quantifiable by the laboratory-scale flow dimensions (n
lab) within individual faults or fractures, with a lower n
lab indicating lower local flow path connectivity. Virtual hydraulic tests were performed on modeled individual fractures to derive a relationship between n
lab and a mappable indicator, the ductility index (DI), defined by the mean stress, groundwater pressure, and rock tensile strength. The derived relationship was verified with data obtained from in-situ hydraulic tests of natural faults in rocks with low matrix permeability, poor swelling capacity, and few fracture mineral fillings, also incorporating the effect of linkage among faults in the field. The test results demonstrated that local flow path connectivity within faults or fractures can be high (n
lab > 1.5) when DI < 2 but is generally low (n
lab < 1.5) when DI > 2, depending on the level of effective-normal-stress-dependent (DI-dependent) fracture-normal displacement. This relationship between n
lab and DI is valid even when the value of DI is varied, or the faults are sheared. These findings can be used to help map spatial variations in local flow path connectivity within faults or fractures from limited borehole data.
Impact of urbanization on groundwater recharge: altered recharge rates and water cycle dynamics for Arusha, Tanzania
Authors: Kiflay Elizabeth , Schirmer Mario , Foppen Jan Willem , Moeck Christian 
Published: January 2025

Abstract
The profound effects of urbanization on groundwater recharge rates are investigated by conducting a comprehensive land use and land cover analysis in Arusha, Tanzania, using the WetSpass model. Between 1995 and 2016, the urban area has expanded from 14 to 45% within the study area. This rapid urbanization has resulted in the conversion of forested areas, agricultural land, shrublands, and bare soil into urban zones. Results indicated that under preurban conditions, groundwater recharge from precipitation was ~116 mm/year, which increased to an average of 148 mm/year by 2016. When accounting for anthropogenic factors such as drinking water leakage and on-site sanitation, recharge further increased to 195 mm/year. These supplementary recharge sources, along with reduced evapotranspiration due to land-use changes, contributed to the increase, despite higher surface runoff. These findings underscore the significance of land use and leakage management in urban areas, as well as the spatial variability in groundwater recharge rates across different urban zones, emphasizing the importance of local factors. This study advances the understanding of the intricate relationship between urbanization and groundwater dynamics, and provides insights for future water resource management in rapidly growing urban regions.
Fertility, pregnancy outcomes, and disease activity during pregnancy in patients with juvenile idiopathic arthritis: a descriptive study
Authors: Sonkurt Ali İhsan , Doğan Gül , Parlar Kerem , Güler Berkay , Deveci Müge , Kasapçopur Özgür , Uğurlu Serdal 
Published: January 2025

Abstract
To investigate the fertility status, pregnancy outcomes, and disease activity during and after pregnancy in patients with juvenile idiopathic arthritis (JIA) currently being followed up at an adult rheumatology clinic. This study included 141 adult patients diagnosed with according to the International League of Associations for Rheumatology criteria, who are now monitored at an adult rheumatology clinic. Data on demographics, disease characteristics, medication history, fertility status, and pregnancy outcomes were collected through medical records and patient interviews. Statistical analysis was performed using Fisher’s exact test to compare categorical variables. Statistical significance was set at p < 0.05. Out of 141 patients (65 male, 76 female), 19 female and 6 male patients attempted to have children. Six male patients fathered ten children without assisted reproductive techniques (ART), while 16 female patients had 19 children. Only one patient couldn’t conceive. The observed pregnancy complications included miscarriage (17.4%), preeclampsia (4.3%), preterm birth (4.3%), and gestational diabetes (4.3%). Flares during or within the first month after labor in 32.2% of pregnancies. There was no statistically significant association between medication use and pregnancy complications or disease activity during pregnancy. JIA does not significantly impact fertility or increase the risk of pregnancy complications and adverse pregnancy outcomes. Disease activity during pregnancy and postpartum flares are manageable without the need for additional precautions. Patients with JIA can pursue pregnancy without significant concerns related to their condition.

Key Points• JIA does not significantly impact fertility in both male and female patients.• JIA does not increase risk of adverse pregnancy outcomes or complications.• JIA disease activity remains stable and manageable during pregnancy and postpartum.
Impact of Fc-gamma receptor IIIA polymorphism on late-onset neutropenia and clinical outcomes in kidney transplant recipients following rituximab induction therapy
Authors: Tashiro Yuki , Hyodo Yoji , Kitamura Satoshi , Fujimoto Takuya , Endo Takahito , Nishioka Shun , Yokoyama Naoki , Hara Takuto , Chiba Koji , Miyake Hideaki 
Published: January 2025

Abstract

Background
This study aimed to investigate the association between the Fc-gamma receptor IIIA (FCGR3A) 158 polymorphism and clinical outcomes in kidney transplantation (KTx) patients. Specifically, we focused on late-onset neutropenia (LON) in ABO-incompatible (ABOi) or HLA-incompatible (HLAi) KTx recipients who underwent rituximab (RTx) desensitization therapy.

Methods
FCGR3A 158F/V polymorphisms were identified in 85 ABOi or HLAi KTx recipients who underwent RTx desensitization at our institution between April 2008 and October 2021. We analyzed these polymorphism groups in relation to their preoperative background and incidence of LON, infection, and rejection. In addition, we examined the risk factors for LON development.

Results
The following FCGR3A 158F/V polymorphisms were identified: FF genotype (n = 45); FV genotype (n = 36), and VV genotype (n = 4). LON occurred in 25 out of 85 recipients within 1 year after KTx, significantly more frequently in patients with the FCGR3A FV + VV genotype (17/40) than in those with the FF genotype (8/45) (p = 0.01). A multivariate analysis identified the V-allele as an independent risk factor for LON (OR, 4.03; 95% CI, 1.38—11.73, p = 0.01). However, there were no significant differences in the incidence rates of post-transplant infection and rejection between the FF and FV + VV genotypes.

Conclusion
Recipients with the FCGR3A 158 V-allele were identified as having a higher risk of developing LON following KTx with RTx desensitization therapy. However, the presence of this V-allele did not affect the safety or efficacy of RTx desensitization before KTx.