bluuebunny/crossref_metadata_2025_split huggingface dataset:
Centrifugal or Roller Blood Pumps for Neonatal Venovenous Extracorporeal Membrane Oxygenation: Extracorporeal Life Support Organization Database Comparison of Mortality and Morbidity
Authors: Ündar Akif , Kunselman Allen R. , Barbaro Ryan P. , Alexander Peta , Patel Krishna , Thomas Neal J. 
Published: April 2023

OBJECTIVES:
To investigate outcomes associated with conventional roller or centrifugal pumps during neonatal venovenous extracorporeal membrane oxygenation (ECMO). Our primary hypothesis is that in comparison with conventional roller-pump support, centrifugal pump use is associated with greater odds of survival. Our secondary hypothesis is that centrifugal pump use is associated with lesser odds of complications.

DESIGN:
Retrospective cohort identified using the Extracorporeal Life Support Organization (ELSO) registry 2016 to 2020 dataset.

SETTING:
All ECMO centers reporting to the ELSO registry.

PATIENTS:
All neonates (≤ 28 d) supported with venovenous ECMO and cannulated via right internal jugular vein using dual-lumen venovenous cannulas and polymethyl pentene membrane oxygenators.

INTERVENTIONS:
None.

MEASUREMENTS AND MAIN RESULTS:
A total of 612 neonates (centrifugal, n = 340; conventional roller, n = 272) were included in the analysis. Using a multivariable logistic regression model, centrifugal pump use—as opposed to roller pump use—was associated with lesser odds of survival (odds ratio [OR], 0.53; 95% CI, 0.33–0.84; p < 0.008). Thrombosis and clots in the circuit components were also associated with lesser odds of survival (OR, 0.28; 95% CI, 0.16–0.60; p < 0.001). We failed to show that hemolysis was an independent variable for survival (OR, 0.60; 95% CI, 0.31–1.19; p = 0.14). The primary diagnosis of neonatal aspiration/meconium aspiration is associated with more than seven-fold greater odds of survival (OR, 7.57; 95% CI, 4.02–15.74; p < 0.001).

CONCLUSIONS:
Contrary to our hypotheses, conventional roller pump use was associated with greater odds of survival. While thrombosis and clots in circuit components were independent variables for lesser odds of survival, further research is needed better to understand the use of centrifugal pumps in neonatal practice.
Textured versus Smooth Tissue Expanders: a Comparison of Complications in 3,526 Breast Reconstructions
Authors: Nelson Jonas A. , Rubenstein Robyn N. , Vorstenbosch Joshua , Haglich Kathryn , Poulton Richard T. , McGriff De’von , Stern Carrie S. , Coriddi Michelle , Cordeiro Peter G. , McCarthy Colleen M. , Disa Joseph J. , Mehrara Babak J. , Matros Evan 
Published: April 2023

Background:
Increased understanding of breast implant-associated anaplastic large cell lymphoma(BIA-ALCL) has led to a shift away from textured breast devices. Few small studies have compared the complication rates of textured and smooth tissue expanders(TEs). The aim of this study was to compare complication profiles in patients undergoing two-stage post-mastectomy breast reconstruction with either textured or smooth TEs.

Methods:
We performed a retrospective review of female patients who underwent immediate breast reconstruction with textured or smooth TEs from 2018 to 2020 at our institution. Rates of seroma, infection/cellulitis, malposition/rotation, exposure, and TE loss were analyzed in the overall cohort and subgroups undergoing prepectoral and subpectoral TE placement. A propensity score matched analysis was used to decrease the effects of confounders comparing textured and smooth TEs.

Results:
We analyzed 3,526 TEs(1,456 textured; 2,070 smooth). More frequent use of acellular dermal matrix(ADM), SPY angiography, and prepectoral TE placement was noted in the smooth TE cohort(p<0.001). Univariate analysis suggested higher rates of infection/cellulitis, malposition/rotation, and exposure in smooth TEs(all p<0.01). Rates of TE loss did not differ. After propensity matching, no differences were noted in infection or TE loss. Prepectoral smooth expanders had increased rates of malposition/rotation.

Conclusions:
TE surface type did not affect rates of TE loss, though increased rates of expander malposition were noted in the smooth prepectoral cohort. Further research is needed to examine BIA-ALCL risk with temporary textured TE exposure to improve decision-making.
Two-Stage Implant-Based Breast Reconstruction Using Intraoperative Fluorescence Imaging: A Propensity Score-Matched Analysis
Authors: Escandón Joseph M. , Christiano Jose G. , Gooch Jessica C. , Olzinski Ann Therese , Prieto Peter A. , Skinner Kristin A. , Langstein Howard N. , Manrique Oscar J. 
Published: April 2023

Background:
Limited comparability between study groups can generate significant selection and observer bias when evaluating the efficacy of SPY system and fluorescence imaging for implant-based breast reconstruction (IBBR). Herein, we compared the surgical outcomes and complications during the first stage of reconstruction between reconstructions intraoperatively evaluated with fluorescence imaging using the SPY system and clinical assessment using a matched analysis.

Methods:
We conducted a retrospective review of patients undergoing total mastectomy and immediate two-stage IBBR with TEs during January 2011 and December 2020. The rate of complication, time for TE-to-implant exchange, and time to start radiotherapy were compared between groups (intraoperative fluorescence imaging versus clinical assessment) using a propensity score-matched analysis.

Results:
After propensity score matching, 198 reconstructions were evaluated. Ninety-nine reconstructions in each group. The median time for TE-to-implant exchange (140-days versus-185 days p=0.476) and time to initiate adjuvant radiotherapy (144-days versus 98-days,p=0.199) were comparable between groups. The 30-day rate of wound-related complications (21% versus 9%, p=0.017) and 30-day rate of wound-related unplanned interventions were significantly higher in reconstructions evaluated with clinical assessment when compared to SPY system (16% versus 5%, p=0.011). A higher 30-day rate of seroma (19% versus 14%, p=0.041) and hematoma (8% versus 0%, p=0.004) were found in reconstructions intraoperatively assessed with SPY.

Conclusion:
After matching, reconstructions evaluated with fluorescence imaging exhibited a lower incidence of early wound-related complications when compared to clinical evaluation alone. Nonetheless, the wise pattern for mastectomy was found to be the only independent predictor associated with early wound-related complications.
Injectable Cartilage Microtissue Regenerated by Autologous Chondrocytes for Nasal Augmentation: A 5-Year Follow-Up Study
Authors: Li Dan , Yin Zongqi , Liu Yu , Li Xin , Dong Wei , Zhang Chen , Ma Jiguang , Zhou Guangdong , Zhang Yixin , Cao Yilin 
Published: April 2023

Background:
A lack of ideal filling materials is a critical limitation in current rhinoplasty. Cartilage sheet regeneration by autologous chondrocytes is expected to provide an ideal source of material. However, the inability to perform minimally invasive transplantation of cartilage sheets has greatly limited the clinical application of this material. In this article, the authors propose the concept of injectable cartilage microtissue (ICM) based on cartilage sheet technology, with the aim of achieving minimally invasive augmentation rhinoplasty in clinical practice.

Methods:
Approximately 1.0 cm2 of posterior auricular cartilage was collected from 28 patients. Isolated chondrocytes were expanded, then used to construct autologous cartilage sheets by high-density seeding and in vitro culture in chondrogenic medium with cytokines (eg, transforming growth factor beta-1 and insulin-like growth factor-1) for 3 weeks. Next, ICM was prepared by granulation of the cartilage sheets; it was then injected into a subcutaneous pocket for rhinoplasty.

Results:
ICM was successfully prepared in all patients, and its implantation efficiently raised the nasal dorsum. Magnetic resonance imaging confirmed that regenerative tissue was present at the injection site; histologic examinations demonstrated mature cartilage formation with typical cartilage lacunae and abundant cartilage-specific deposition of extracellular matrix. Excellent or good postoperative patient satisfaction results were achieved in 83.3% of patients over 5 years of follow-up. Obvious absorption of grafts occurred in only two patients (8.3%).

Conclusions:
These results demonstrated that ICM could facilitate stable cartilage regeneration and long-term maintenance in the human body; the implantation of ICM enabled natural augmentation of the depressed nasal dorsum.

CLINICAL QUESTION/LEVEL OF EVIDENCE:
Therapeutic, IV.
Indole-3-Propionic acid, A Gut Microbiota Metabolite, Protects Against the Development of Postoperative Delirium
Authors: Zhou Xue , Wu Xinbo , Wu Yan , Yang Liuyue , Shi Eleanor , Ding Weihua , Chen Liang , Shi Xu , Feng Xia , Su Chienwen , You Zerong , Xia Jianguo , Chen Cynthia , Yeliseyev Vladimir , Bry Lynn , Xia Suyun , Huang Peigen , Meng Jiawei , Houle Timothy , Akeju Oluwaseun , Mao Jianren , Gerszten Robert , Chen Qian , Xie Zhongcong , Shen Shiqian 
Published: April 2023

Objective:
Determine preoperative gut microbiota metabolites that may be associated with postoperative delirium (POD) development in patients and further study in rodents.

Summary Background Data:
POD occurs in 9-50% of older patients undergoing anesthesia/surgery but lacks effective treatments or prevention. High throughput metabolomics using liquid chromatography with tandem mass spectrometry have accelerated disease-related biomarkers discovery. We performed metabolomic studies in humans to identify potential metabolite biomarkers linked to POD and examined potential mechanisms in rodents.

Methods:
We performed a prospective observational cohort study to examine the metabolomic changes that were associated with the development of POD. Then the gut microbiota-related metabolomic changes were recapitulated by gut microbiota perturbation in rodents. POD was assessed in mice using a battery of behavioral tests including novel objective test, Y maze test, open field test, and buried food test. The mechanisms through which gut microbiota-related metabolomic changes influenced POD were examined using chemogenetics.

Results:
IPA is a gut microbiota metabolite that belongs to the indole family. Baseline plasma levels of IPA were significantly inversely correlated with the onset of POD in 103 (17 cases) human individuals. This relationship was validated in preclinical mouse models for POD: reducing IPA levels through gut microbiota perturbation promoted POD-like behavior. More importantly, IPA administration deterred POD-like behavior. Colonization of germ-free mice with mutant Clostridium sporogenes that did not produce IPA promoted POD-like behavior. Chemogenetic studies revealed that the protective effect of IPA in mice was mediated, in part, by peroxisome proliferator-activated receptor gamma coactivator 1-alpha in hippocampal interneurons.

Conclusions:
Gut microbiota-derived IPA is an important molecule implicated in the pathogenesis of POD, which could potentially be harnessed for POD prevention. (270 words)
Lesion-specific suppression of YAP/TAZ by biomimetic nanodrug ameliorates atherosclerosis development
Authors: Huang Hui-Chun , Wang Ting-Yun , Rousseau Joshua , Mungaray Michelle , Michaud Chamonix , Plaisier Christopher , Chen Zhen Bouman , Wang Kuei-Chun 
Published: April 2023

AbstractAtherosclerosis, characterized by the buildup of lipid-rich plaque on the vessel wall, is the primary cause of myocardial infarction and ischemic stroke. Recent studies have demonstrated that dysregulation of yes-associated protein 1 (YAP) and transcriptional coactivator with PDZ-binding domain (TAZ) contributes to plaque development, making YAP/TAZ potential therapeutic targets. However, systemic modulation of YAP/TAZ expression or activities risks serious off-target effects, limiting clinical applicability. To address the challenge, this study develops monocyte membrane-coated nanoparticles (MoNP) as a drug delivery vehicle targeting activated endothelium lining the plaque surface and utilizes MoNP to deliver verteporfin (VP), a potent YAP/TAZ inhibitor, for lesion-specific treatment of atherosclerosis. The results reveal that MoNP significantly enhance payload delivery to inflamed endothelial cells (EC) while avoiding phagocytic cells, and preferentially accumulate in atherosclerotic regions. MoNP-mediated delivery of VP substantially reduces YAP/TAZ expression, suppressing inflammatory gene expression and macrophage infiltration in cultured EC and mouse arteries exposed to atherogenic stimuli. Importantly, this lesion-targeted VP nanodrug effectively decreases plaque development in mice without causing noticeable histopathological changes in major organs. Collectively, these findings demonstrate a plaque-targeted and pathway-specific biomimetic nanodrug, potentially leading to safer and more effective treatments for atherosclerosis.
Structure determination of a DNA crystal by MicroED
Authors: Haymaker Alison , Bardin Andrey A. , Gonen Tamir , Martynowycz Michael W. , Nannenga Brent L. 
Published: April 2023

SUMMARYMicrocrystal electron diffraction (MicroED) is a powerful tool for determining high-resolution structures of microcrystals from a diverse array of biomolecular, chemical, and material samples. In this study, we apply MicroED to DNA crystals, which have not been previously analyzed using this technique. We utilized the d(CGCGCG)2DNA duplex as a model sample and employed cryo-FIB milling to create thin lamella for diffraction data collection. The MicroED data collection and subsequent processing resulted in a 1.10 Å resolution structure of the d(CGCGCG)2DNA, demonstrating the successful application of cryo-FIB milling and MicroED to the investigation of nucleic acid crystals.
A pyridyl-furan series developed from Open Global Health Library blocks red blood cell invasion and protein trafficking inPlasmodium falciparumthrough potential inhibition of the parasite’s PI4KIIIb enzyme
Authors: Ling Dawson B. , Nguyen William , Looker Oliver , Razook Zahra , McCann Kirsty , Barry Alyssa E. , Scheurer Christian , Wittlin Sergio , Bullen Hayley E. , Crabb Brendan S. , Sleebs Brad E. , Gilson Paul R. 
Published: April 2023

ABSTRACTWith resistance increasing to current antimalarial medicines, there is an urgent need to discover new drug targets and to develop new medicines against these targets. We therefore screened the Open Global Health Library of Merck KGaA, Darmstadt, Germany of 250 compounds against the asexual blood stage of the deadliest malarial parasitePlasmodium falciparum,from which eight inhibitors with low micromolar potency were found. Due to its combined potencies against parasite growth and inhibition of red blood cell invasion, the pyridyl-furan compound OGHL250, was prioritised for further optimisation. The potency of the series lead compound (WEHI-518) was improved 250-fold to low nanomolar levels against parasite blood-stage growth. Parasites selected for resistance to a related compound MMV396797, were also resistant to WEHI-518 as well as KDU731, an inhibitor of the phosphatidylinositol kinase PfPI4KIIIB, suggesting this kinase is the target of the pyridyl-furan series. Inhibition of PfPI4KIIIB blocks multiple stages of the parasite’s life cycle and other potent inhibitors are currently under preclinical development. MMV396797-resistant parasites possess an E1316D mutation in PfPKI4IIIB which clusters with known resistance mutations of other inhibitors of the kinase. Building upon earlier studies which showed that PfPI4KIIIB inhibitors block the development of the invasive merozoite parasite stage, we show that members of the pyridyl-furan series also block invasion and/or the conversion of merozoites into ring-stage intracellular parasites through inhibition of protein secretion and export into red blood cells.
Membrane binding of endocytic myosin-1s is inhibited by a class of ankyrin repeat proteins
Authors: Willet Alaina H. , Chen Jun-Song , Ren Liping , Gould Kathleen L. 
Published: April 2023

AbstractMyosin-1s are monomeric actin-based motors that function at membranes. Myo1 is the single myosin-1 isoform inSchizosaccharomyces pombethat works redundantly with Wsp1-Vrp1 to activate the Arp2/3 complex for endocytosis. Here, we identified Ank1 as an uncharacterized cytoplasmic Myo1 binding partner. We found that inank1Δcells, Myo1 dramatically redistributed from endocytic patches to decorate the entire plasma membrane and endocytosis was defective. Biochemical analysis and structural predictions suggested that the Ank1 ankyrin repeats bind the Myo1 lever arm and the Ank1 acidic tail binds the Myo1 TH1 domain to prevent TH1-dependent Myo1 membrane binding. Indeed, Ank1 over-expression precluded Myo1 membrane localization and recombinant Ank1 blocked purified Myo1 liposome binding in vitro. Based on biochemical and cell biology analyses, we propose budding yeast Ank1 and human OSTF1 are functional Ank1 orthologs and that cytoplasmic sequestration by small ankyrin repeat proteins is a conserved mechanism regulating myosin-1s in endocytosis.SummaryFission yeast long-tailed myosin-1 binds Ank1. Ank1 ankyrin repeats associate with the Myo1 lever arm and Ank1 acidic tail binds the Myo1 TH1 domain to inhibit Myo1 membrane binding. Ank1 orthologs exists in budding yeast (Ank1) and humans (OSTF1).
Mapping resistance-associated anthelmintic interactions in the model nematodeCaenorhabditis elegans
Authors: Rehborg Elena G. , Wheeler Nicolas J , Zamanian Mostafa 
Published: April 2023

AbstractParasitic nematodes infect billions of people and are mainly controlled by anthelmintic mass drug administration (MDA). While there are growing efforts to better understand mechanisms of anthelmintic resistance in human and animal populations, it is unclear how resistance mechanisms that alter susceptibility to one drug affect the interactions and efficacy of drugs used in combination. Mutations that alter drug permeability across primary nematode barriers have been identified as potential resistance mechanisms using the model nematodeCaenorhabditis elegans. We leveraged high-throughput assays in this model system to measure altered anthelmintic susceptibility in response to genetic perturbations of potential cuticular, amphidial, and alimentary routes of drug entry. Mutations in genes associated with these tissue barriers differentially altered susceptibility to the major anthelmintic classes (macrocyclic lactones, benzimidazoles, and nicotinic acetylcholine receptor agonists) as measured by animal development. We investigated two-way anthelmintic interactions acrossC. elegansgenetic backgrounds that confer resistance or hypersensitivity to one or more drugs. We observe that genetic perturbations that alter susceptibility to a single drug can shift the drug interaction landscape and lead to the appearance of novel synergistic and antagonistic interactions. This work establishes a framework for investigating combinatorial therapies in model nematodes that can potentially be translated to amenable parasite species.
Exploring Microbial Diversity and Functional Potential along the Bay of Bengal Coastline in Bangladesh: Insights from Amplicon Sequencing and Shotgun Metagenomics
Authors: Akter Salma , Rahman M. Shaminur , Ali Hazrat , Minch Benjamin , Mehzabin Kaniz , Siddique Md. Moradul , Galib Syed Md. , Yesmin Farida , Azmuda Nafisa , Adnan Nihad , Hasan Nur A , Rahman Sabita Rezwana , Moniruzzaman Mohammad , Ahmed Md Firoz 
Published: April 2023

AbstractAlthough the Bay of Bengal (BoB) is the world’s largest bay, possessing distinct physiochemical properties, it has garnered little research focus concerning its microbial diversity and ecological importance. Here, we present amplicon (16S and 18S) profiling and shotgun metagenomics data regarding microbial communities from BoB’s eastern coast, viz., Saint Martin and Cox’s Bazar, Bangladesh. From the 16S sequencing data, Proteobacteria appeared to be the dominant phylum in both locations, withAlteromonas,Methylophaga,Anaerospora,Marivita, andVibriodominating in Cox’s Bazar andPseudoalteromonas,Nautella,Marinomonas,Vibrio, andAlteromonasdominating the Saint Martin site. From the 18S sequencing data, Ochrophyta, Chlorophyta, and Protalveolata appeared among the most abundant eukaryotic divisions in both locations, with significantly higher abundance of Choanoflagellida, Florideophycidae, and Dinoflagellata in Cox’s Bazar. Functional annotations revealed that the microbial communities in these samples harbor genes for biofilm formation, quorum sensing, xenobiotics degradation, antimicrobial resistance, and a variety of other processes. Together, these results provide the first molecular insight into the functional and phylogenetic diversity of microbes along the BoB coast of Bangladesh and lay the foundation for further in-depth assessment of microbial community dynamics and functional potential in the context of global change in this region.
Preparation of physiologically active inside-out vesicles from plant inner mitochondrial membranes
Authors: Ehmke Leander , Hause Gerd , Klösgen Ralf Bernd , Bennewitz Bationa 
Published: April 2023

AbstractFor many metabolites, the major barrier between cytosol and mitochondrial matrix is the inner envelope membrane of mitochondria, the site of the respiratory electron transport chain. In consequence, it houses numerous transporters which facilitate the controlled exchange of metabolites, ions, and even proteins between these cellular compartments. While their import into the organelle can be studied with isolated mitochondria or mitoplasts, the analysis of their export from the matrix into the intermembrane space or even the cytosol demands for more sophisticated approaches. Among those, inside-out inner membrane vesicles are particularly useful, since they allow the direct presentation of the potential export substrates to the membrane without prior import into the organelle. Here we present a protocol for the isolation of such inside-out vesicles of the inner envelope membrane of plant mitochondria based on repeated freeze/thaw-cycles of freshly prepared mitoplasts. Electron microscopy and Western analysis could show that the majority of the vesicles have single envelope membranes in an inside-out topology. The vesicles are furthermore physiologically active, as demonstrated by assays measuring the enzymatic activities of Complex I (NADH dehydrogenase), Complex V (ATP synthase) and the mitochondrial processing peptidase (MPP) associated with Complex III. Hence, the method presented here provides a good basis for further studies of the inner mitochondrial envelope membrane and mitochondrial export processes.