bluuebunny/crossref_metadata_2025_split huggingface dataset:
CCND2 Modified mRNA Activates Cell Cycle of Cardiomyocytes in Hearts With Myocardial Infarction in Mice and Pigs
Authors: Sun Jiacheng , Wang Lu , Matthews Rachel C. , Walcott Gregory P. , Lu Yu-An , Wei Yuhua , Zhou Yang , Zangi Lior , Zhang Jianyi 
Published: August 2023

BACKGROUND:
Experiments in mammalian models of cardiac injury suggest that the cardiomyocyte-specific overexpression of CCND2 (cyclin D2, in humans) improves recovery from myocardial infarction (MI). The primary objective of this investigation was to demonstrate that our specific modified mRNA translation system (SMRTs) can induce CCND2 expression in cardiomyocytes and replicate the benefits observed in other studies of cardiomyocyte-specific CCND2 overexpression for myocardial repair.

METHODS:
The CCND2-cardiomyocyte-specific modified mRNA translation system (cardiomyocyte SMRTs) consists of 2 modRNA constructs: one codes for CCND2 and contains a binding site for L7Ae, and the other codes for L7Ae and contains recognition elements for the cardiomyocyte-specific microRNAs miR-1 and miR-208. Thus, L7Ae suppresses CCND2 translation in noncardiomyocytes but is itself suppressed by endogenous miR-1 and -208 in cardiomyocytes, thereby facilitating cardiomyocyte-specific CCND2 expression. Experiments were conducted in both mouse and pig models of MI, and control assessments were performed in animals treated with an SMRTs coding for the cardiomyocyte-specific expression of luciferase or green fluorescent protein (GFP), in animals treated with L7Ae modRNA alone or with the delivery vehicle, and in Sham-operated animals.

RESULTS:
CCND2 was abundantly expressed in cultured, postmitotic cardiomyocytes 2 days after transfection with the CCND2-cardiomyocyte SMRTs, and the increase was accompanied by the upregulation of markers for cell-cycle activation and proliferation (eg, Ki67 and Aurora B kinase). When the GFP-cardiomyocyte SMRTs were intramyocardially injected into infarcted mouse hearts, the GFP signal was observed in cardiomyocytes but no other cell type. In both MI models, cardiomyocyte proliferation (on day 7 and day 3 after treatment administration in mice and pigs, respectively) was significantly greater, left-ventricular ejection fractions (days 7 and 28 in mice, days 10 and 28 in pigs) were significantly higher, and infarcts (day 28 in both species) were significantly smaller in animals treated with the CCND2-cardiomyocyte SMRTs than in any other group that underwent MI induction.

CONCLUSIONS:
Intramyocardial injections of the CCND2-cardiomyocyte SMRTs promoted cardiomyocyte proliferation, reduced infarct size, and improved cardiac performance in small and large mammalian hearts with MI.
The Great Khan’s Continent: On Taḥqīq and Rashīd al-Dīn’s Discovery of China
Authors: Calzolaio Francesco 
Published: August 2023

Abstract
The Persian scholar and state official Rashīd al-Dīn (d. 1318) devoted a considerable part of his literary output to the study of China (Khatā’) and its foremost cultural achievements. The most distinctive aspect of these writings is their sheer novelty. Indeed, Rashīd al-Dīn is the first Islamicate author to systematically investigate such subjects with reference to textual sources and informants from East Asia. This article discusses the motives and methods underlying Rashīd al-Dīn’s intellectual engagement with China, ultimately framing him as an example of (post-)Mongol muḥaqqiq, or “taḥqīq practitioner,” and his writings about China as an emblematic case of Mongol-sponsored, taḥqīq-infused scholarship.
On Taḥqīq, Space Travel, and the Discovery of Jetlag: Post-Mongol Trajectories of Modern Spatial Thinking
Authors: Casale Giancarlo 
Published: August 2023

Abstract
Before the invention of modern air travel, the phenomenon we call “jetlag” was known as the “Circumnavigator’s Paradox.” It was first observed empirically by European mariners in the sixteenth century, who noticed a missing day in their ship logs after circumnavigating the globe. But two centuries earlier, the theoretical possibility of such an observation was demonstrated by the Arab statesman and polymath Abu’l-Fidā (d. 1331) in his treatise on world geography, the Taqwīm al-Buldān or “Arrangement of Countries.” Within the context of this special issue on Cultures of Taḥqīq, this article argues that Abu’l-Fidā insight was a quintessential expression of the epistemology of taḥqīq as practiced in the immediate aftermath of the Mongol conquests, with profound implications for the latter history of geography, cartography, and modern spatial thinking.
The Truth is out There (and also in Here): Taḥqīq as an Investigative Modality in Mughal Culture and Scholarship
Authors: Kinra Rajeev 
Published: August 2023

Abstract
This article offers a brief examination of various uses and senses of the term taḥqīq (ascertaining the truth; investigation; verification) in Mughal intellectual circles in the sixteenth-eighteenth centuries. Often contrasted with another term, taqlīd (copying; imitation), taḥqīq could signify different kinds of epistemological commitments – and therefore different kinds of truth-claims – depending on the context in which it was being deployed. Here we examine the ways in which the notion of taḥqīq figured prominently in Mughal debates about religious toleration, in Mughal scholarly culture, and even in Mughal notions of kingship, justice, and statecraft. Along the way, we will also use Mughal ideas about taḥqīq as an opportunity to intervene in larger debates about the nature of global early modernity.
“Listen to Him!”: Angelic and Divine Typology in Mark’s Transfiguration Account
Authors: Robinson Jonathan Rivett 
Published: August 2023

Abstract
In this study I will argue that, while Matthew and Luke’s redaction of the Markan Transfiguration present Jesus as the antitype of Moses, Mark’s own account does not. Rather, Mark uses typology to narrate Jesus into the stories of Elijah and Moses, both of whom are described in the Jewish scriptures as ascending a mountain to talk with God in narratives in which the Angel of YHWH also features. Distinctive features of Mark’s account suggest that Mark wishes to associate Jesus, not with Moses, but with YHWH and his angel, using the scriptural ambiguity between the Angel of YHWH and Israel’s God to generate a similar ambiguity around the divine identity of Jesus. Consequently, Mark’s Transfiguration presents a “higher” Christology than that of the Transfigurations in the other Synoptic Gospels. In Mark’s Transfiguration, Jesus is compared, not to human prophets, but to the anthropomorphic manifestations of Israel’s God at Sinai and Horeb.
Multidecadal Oceanic Modulation of Summer Precipitation in North China in 1200-Year Global Climate Simulations
Authors: Tong Xuan , Yan Zhongwei , Zhou Wen , Xia Jiangjiang , Quan Xiaowei 
Published: August 2023

Abstract
The multidecadal decrease of precipitation in North China since the 1960s has caused a significant impact on the ecological environment and social development. It has become a key concern for meteorologists and national decision-makers to understand what is driving this. To better understand the oceanic impact on the interdecadal variation of summer precipitation in North China, we analyze two 1200-yr global climate simulations from state-of-the-art coupled models with an explainable convolutional neural network (CNN). The accuracy of the CNN model in correctly diagnosing the increased and decreased summer precipitation anomaly in North China reaches 98% and 96.6% in two CMIP6 models, respectively. We find that a sea surface temperature anomaly (SSTA) in the northern Pacific, associated with the Pacific decadal oscillation (PDO), and an SSTA in the northern Atlantic, associated with the Atlantic multidecadal oscillation (AMO), play essential roles in the interdecadal variation of summer precipitation in North China. The modulations of the PDO and AMO on summer precipitation in North China are consistent with the results derived from limited observations and simulations, which means these mechanisms are robust. In particular, when the PDO is in a negative phase and the AMO is in a positive phase, the positive anomaly of precipitation and the associated circulation anomalies are intensified. This study also confirms that with the explainable deep learning method, a convolutional neural network can be used to identify multidecadal variability in North China summer precipitation, although limited datasets such as observational records make it more challenging to apply deep learning methods.
Are slaughterhouse-obtained livers suitable for use in ex vivo perfusion research?
Authors: Ruppelt Alicia , Pijnenburg Iris , Pappers Claire , Samsom Roos-Anne , Kock Linda , Grinwis Guy C. M. , Spee Bart , Rasponi Marco , Stijnen Marco 
Published: August 2023

Objectives The success of the ex vivo machine perfusion of pig livers used for preclinical research depends on organ quality and availability. In this study, we investigated whether livers obtained from slaughterhouses are suitable and equivalent to livers obtained from laboratory pigs. Methods Livers were obtained from slaughterhouse pigs stunned by electrocution or CO2 inhalation and from laboratory pigs. For the latter group, 45 minutes of warm ischemia was mimicked for a subgroup, ensuring a valid comparison with slaughterhouse-derived livers. Results Livers from CO2-stunned pigs showed lower indocyanine green clearance and bile production, higher blood lactate and potassium concentrations, and higher alanine aminotransferase activities than electrically stunned pigs. Furthermore, livers from electrically stunned pigs, and livers from laboratory pigs, subjected or not to warm ischemia, showed similar performance in terms of perfusion and metabolism. Conclusion For an ex vivo liver model generated using slaughterhouse pigs, electrical stunning is preferable to CO2 stunning. Livers from electrically stunned slaughterhouse pigs performed similarly to laboratory pig livers. These findings support the use of livers from electrically stunned slaughterhouse pigs, which may therefore provide an alternative to livers obtained from laboratory pigs, consistent with the principle of the 3Rs.
Comparison Between Radial and Femoral Artery Access for Transarterial Chemoembolization in Patients With Hepatocellular Carcinoma
Authors: Hedjoudje Mohamed , Barat Maxime , Dohan Anthony , Lucas Alexandre , Dautry Raphael , Coriat Romain , Marchese Ugo , Pol Stanislas , Parlati Lucia , Soyer Philippe 
Published: August 2023

Purpose The purpose of this study was to compare the technical success rate, the selectivity of transarterial chemoembolization (TACE), the complication rate, the radiation dose given to the patients and the hospitalization stay between TACE performed using femoral artery approach (FAA) and TACE performed using radial artery approach (RAA) in patients with hepatocellular carcinoma (HCC). Methods Between June 2020 and April 2022, 49 patients with HCC who underwent 116 TACEs (75 using FAA and 41 using RAA) were included. Differences in technical success rate, selectivity of micro-catheterization, radiation dose given to the patients, fluoroscopy time, hospitalization stay duration, and complication rate were compared between FAA and RAA using Fisher exact or Student t tests. Results No differences in technical success rates were found between RAA (93%; 39/41 TACEs) and FAA (100%; 75/75 TACEs) ( P = .12). There were no differences between the two groups in terms of selectivity of catheterization, radiation dose, fluoroscopy time and hospitalization stay duration. Five patients had Grade 2 complications (hematoma) after FAA vs. one patient with one Grade 1 complication (radial artery occlusion) after RAA (5/75 [7%] vs. 1/41 [2%], respectively; P = .42). No major arterial access site complications occurred with FAA or RAA. Conclusions This study confirms that RAA is a safe approach that does not compromise the technical efficacy and the selectivity of TACE compared to FAA in patients with HCC.
Development and Application of qPCR Assay for Species-specific Identification of Two Animal-derived Tonics: Vinegar-quenched Testudinis Carapax et Plastrum and Vinegar-quenched Trionycis Carapax
Authors: Xu Chao , Chen Xiang , Han Jing , Chen Yufei , Ge Chongyu , Wang Liwei , Yang Rong , Shen Yuping , Yang Huan 
Published: August 2023

Background Vinegar-quenched Testudinis Carapax et Plastrum (TCP) and vinegar-quenched Trionycis Carapax (TC) are made from the shells of Chinemys reevesii and Trionyx sinensis. However, some cheap substitutes from non-medicinal species such as Trachemys scripta, Mauremys sinensis, Chelydra serpentina, and Apalone ferox are often seen on the market, which exposes public health to high risk. Conventional methods are not applicable for the identification of these two products since their intrinsic constituents are largely destroyed after being highly processed. Objectives A new quantitative polymerase chain reaction (qPCR) method was developed to detect DNA components of Vinegar-quenched TCP and vinegar-quenched TC based on simple sequence repeat differential genes of animal-derived mitochondria. Materials and Methods Six species-specific primer reactions were designed and further validated for specificity, sensitivity, and repeatability. Finally, the developed method was used to assess the authenticity of their commercial products. Results The assay exhibited good specificity, and the limit of detection was above 1 × 102 copies/µL. There was good linearity in the concentration, ranging from 1 × 102 to 1 × 107 copies/µL. In addition, the method was validated through repeatability assessment (coefficient variation <1%). While qPCR was applied for the analysis of 36 batches of commercial products of these two tonics, it could effectively identify the authenticity of the products, and consequently, seven batches were fake or adulterated. Conclusion This newly proposed method is promising for the quality evaluation of highly processed animal-derived Chinese medicines, which will assist in ensuring safety and efficacy in clinical practice and protect fair trade in the market.
Cleft Palate in Apert Syndrome: A Descriptive Study of Incidence and Surgical Outcome
Authors: Andiné Alicia , Tarnow Peter , Boivie Patrik 
Published: August 2023

Objective Apert syndrome (AS) is a rare congenital craniofacial disorder that requires a multidisciplinary approach to treatment and multiple surgeries. Given that cleft palate (CP) is presented in some of these cases, this poses an additional risk of aggravating obstructed airways after closure. The timing and outcome of CP repair in these patients remains disputed and requires additional attention. Design This retrospective analysis included patients diagnosed with CP and AS, born between 1950 and 2020, and treated at our institution. Data were collected from medical records and evaluated using descriptive statistics. Setting Data analyses were conducted at Sahlgrenska University Hospital in Gothenburg, Sweden. Patients/Participants A registry of 83 patients with AS resulted in a cohort of 26 patients also presenting with CP. Main Outcome Measures Postoperative complications, requirement for intensive care, and reoperations following CP repair. Results CP incidence among all registered patients was 31%. Patients undergoing CP repair at low age (mean: 22.5 months) tended to experience more frequent postoperative complications and requirements for intensive care. Among the evaluated cohort with medical records describing CP repair ( n = 14), 29% experienced postoperative complications, all of which involved aggravation of obstructed airways. Conclusions This study highlights the importance of airway assessment before and after CP repair in AS. The findings suggest that surgical outcomes might benefit from postponing CP repair, avoiding combined surgeries, and operating in two stages when indicated. However, additional and larger studies are required.
Compared Profile of Late-Onset Versus Childhood-Onset ADHD: A Case-Control Study Among Treatment-Seeking Adult Patients
Authors: Jurek Lucie , Montègue Solène , Larrieu Agathe , Icard Christophe , Rolland Benjamin 
Published: August 2023

Objective: To compare the characteristics of childhood-onset versus late-onset Attention Deficit Hyperactivity Disorder (ADHD) in a sample of treatment-seeking patients. Method: Among total of 101 adult patients who were recently diagnosed for ADHD, using the Diagnostic Interview for Adult ADHD (DIVA 2.0), 56 subjects exhibited childhood-onset ADHD, versus 45 displayed late-onset ADHD. Both groups were compared according to their sociodemographic, clinical, and neuropsychological features, providing crude ( OR) and adjusted odds ratios (aOR), and their 95% confidence intervals [95% CI]. Results: Compared to late-onset ADHD, patients with childhood-onset had a lower educational score, ( OR = 0.52; 95% CI [0.35, 0.76]), a greater score of impulsivity (aOR = 1.09; 95% CI [1.03, 1.16]), an increased number of hyperactive-impulsive ADHD symptoms (aOR = 1.9; 95% CI [1.46, 2.47]), and higher rates childhood trauma (aOR = 1.07; 95% CI [1.01, 1.13]), cannabis use disorder (aOR = 1.07; 95% CI [1.01, 1.13]), and working memory impairment. No difference was observed concerning age, sex, psychiatric symptoms, quality of life, and autonomy. Conclusion: Childhood-onset adult ADHD displayed a more severe profile, relative to late-onset ADHD.
Heterogeneous programming using OpenMP and CUDA/HIP for hybrid CPU-GPU scientific applications
Authors: Gonzalez Tallada Marc , Morancho Enric 
Published: August 2023

Hybrid computer systems combine compute units (CUs) of different nature like CPUs, GPUs and FPGAs. Simultaneously exploiting the computing power of these CUs requires a careful decomposition of the applications into balanced parallel tasks according to both the performance of each CU type and the communication costs among them. This paper describes the design and implementation of runtime support for OpenMP hybrid GPU-CPU applications, when mixed with GPU-oriented programming models (e.g. CUDA/HIP). The paper describes the case for a hybrid multi-level parallelization of the NPB-MZ benchmark suite. The implementation exploits both coarse-grain and fine-grain parallelism, mapped to compute units of different nature (GPUs and CPUs). The paper describes the implementation of runtime support to bridge OpenMP and HIP, introducing the abstractions of Computing Unit and Data Placement. We compare hybrid and non-hybrid executions under state-of-the-art schedulers for OpenMP: static and dynamic task schedulings. Then, we improve the set of schedulers with two additional variants: a memorizing-dynamic task scheduling and a profile-based static task scheduling. On a computing node composed of one AMD EPYC 7742 @ 2.250 GHz (64 cores and 2 threads/core, totalling 128 threads per node) and 2 × GPU AMD Radeon Instinct MI50 with 32 GB, hybrid executions present speedups from 1.10× up to 3.5× with respect to a non-hybrid GPU implementation, depending on the number of activated CUs.
Impact of parastomal hernia on colostomy costs at 1 year: Secondary analysis of a randomized clinical trial (STOMAMESH)
Authors: Odensten Christoffer , Gunnarsson Ulf , Johansson Jeaneth , Näsvall Pia 
Published: August 2023

Background and aims: Parastomal hernia (PSH) is a common complication after the creation of a colostomy, with a prevalence of approximately 50%. Despite the high frequency, little is known how PSH affects the cost of colostomy care. The hypothesis in this study was that PSH increases the cost of colostomy care compared with not having a PSH. Methods: Two groups with (N = 61) and without (n = 147) PSH were compared regarding costs of stoma appliances and visits. The population from a large randomized trial comparing construction of colostomy with or without prophylactic mesh (STOMAMESH) was used and cross-matched with health economic data from the National Pharmaceutical Register, 1 year after initial surgery. Results: Patients with and without a PSH were similar in basic demographic data. No difference in cost of stoma appliances (with PSH 2668.3 EUR versus no PSH 2724.5 EUR, p = 0.938) or number of visits to a stoma therapist ( p = 0.987) was seen, regardless of the presence or not of a PSH. Conclusions: PSH appears not to affect costs due to colostomy appliances or the need to visit a stoma therapist, in the first year. The lesson to be learnt is that PSHs are not a driver for costs. Other factors may be determinants of the cost of a colostomy, including manufacturers’ price and persuasion, means of procurement, and presence of guidelines.
Changes in lung cancer staging and emergency presentations during the first year of the COVID-19 pandemic
Authors: Vella Claire , Parvez Wadood , Ashraf Asif , Ajmal Syed , Sudhir Rajini , Agrawal Sanjay , Bennett Jonathan , Tufail Muhammad 
Published: August 2023

Aim: This study retrospectively analyses the impact of the 1st year of the COVID-19 pandemic on route of presentation and staging in lung cancer compared to the 2 years before and after implementation of the Leicester Optimal Lung Cancer Pathway (LOLCP) in Leicester, United Kingdom. Method: Electronic databases and hospital records were used to identify all patients diagnosed with lung cancer in 2018 (pre-LOLCP), 2019 (post-LOLCP), and March 2020–2021 (post-COVID-19 lockdown). Information regarding patient characteristics, performance status, stage, and route of diagnosis was documented and analysed. Emergency presentation was defined as diagnosis of new lung cancer being made after unscheduled attendance to urgent or emergency care facility. Results: Following implementation of the LOLCP pathway, there was a significant decrease in emergency presentations from 26.8 to 19.6% ( p = 0.002) with a stage shift from 33.9% early stage disease to 40.3%. These improved outcomes were annulled during the COVID-19 pandemic, with emergency presentations increasing to 38.9% ( p < 0.001) and a reduction in early-stage lung cancer diagnoses to 31.5%. There was a 61% decline in 2 week wait referrals but no significant decline in the LOLCP direct-to-CT referrals. Conclusion: We have demonstrated a significant increase in late-stage lung cancer diagnoses and emergency presentations during the first year of the COVID-19 pandemic. The causes for these changes are likely to be multifactorial. The long-term effect on lung cancer mortality remains to be seen and is an important focus of future study.
Potentiality of Organosulfur Compounds Against SARS-CoV-2-Coinfected Bacteria Streptococcus pyogenes and S pneumoniae: A Cross-Platform Analysis from Computational Chemistry
Authors: Thai Nguyen Minh , Bui Thanh Q. , Quy Phan Tu , Thanh Hai Nguyen Thi , To Dao-Cuong , Quang Duong Tuan , Co Nguyen Quang , Triet Nguyen Thanh , Ai Thuan Nguyen Thi , To Nhi Nguyen Thanh , Tham Vo Mong , Ai Nhung Nguyen Thi 
Published: August 2023

Objective SARS-CoV-2 coinfection with Streptococcus pyogenes and S pneumoniae increases disease severity, thus deserving more in-depth understanding. Methods The potential of garlic extract against the bacteria has been theoretically examined and extended to synergic predictions on SARS-CoV-2 Delta and Omicron variants. Results Gas chromatography–mass spectrometry characterized five main components in the extract: benzeneacetaldehyde (A1; 5.65%), diallyl disulfide (A2; 19.27%), 1-allyl-3-methyltrisulfane (A3; 21.30%), methyl salicylate (A4; 7.49%), and diallyl trisulfide (A5; 39.26%). Bioassay-based inhibition zones were 8 ± 2 mm, and MIC values 64 mg·mL−1 ( S pneumoniae) and 128 mg·mL−1 ( S pyogenes). Density functional theory suggested the organosulfur components as the most promising inhibitors with electrophilic sites at their sulfur atoms. Molecular docking simulation agreed with their potential against S pneumoniae/ S pyogenes luxS proteins and Delta/Omicron SARS-CoV-2 spike proteins, given by all docking score values being <−13 kcal·mol−1. QSARIS and ADMET predicted their bio- and pharma-favored properties. Conclusion The consistency of a variety of computational outputs justifies the dietary–supplemental potential of garlic essential oil based on its organosulfur ingredients.