bluuebunny/crossref_metadata_2025_split huggingface dataset:
Immediate effects of acute Mars mission equivalent doses of SEP and GCR radiation on the murine gastrointestinal system-protective effects of curcumin-loaded nanolipoprotein particles (cNLPs)
Authors: Diaz Jonathan , Kuhlman Bradford M. , Edenhoffer Nicholas P. , Evans Angela C. , Martin Kelly A. , Guida Peter , Rusek Adam , Atala Anthony , Coleman Matthew A. , Wilson Paul F. , Almeida-Porada Graça , Porada Christopher D. 
Published: May 2023

Introduction: Missions beyond low Earth orbit (LEO) will expose astronauts to ionizing radiation (IR) in the form of solar energetic particles (SEP) and galactic cosmic rays (GCR) including high atomic number and energy (HZE) nuclei. The gastrointestinal (GI) system is documented to be highly radiosensitive with even relatively low dose IR exposures capable of inducing mucosal lesions and disrupting epithelial barrier function. IR is also an established risk factor for colorectal cancer (CRC) with several studies examining long-term GI effects of SEP/GCR exposure using tumor-prone APC mouse models. Studies of acute short-term effects of modeled space radiation exposures in wildtype mouse models are more limited and necessary to better define charged particle- induced GI pathologies and test novel medical countermeasures (MCMs) to promote astronaut safety.Methods: In this study, we performed ground-based studies where male and female C57BL/6J mice were exposed to γ-rays, 50 MeV protons, or 1 GeV/n Fe-56 ions at the NASA Space Radiation Laboratory (NSRL) with histology and immunohistochemistry endpoints measured in the first 24 h post-irradiation to define immediate SEP/GCR-induced GI alterations.Results: Our data show that unlike matched γ-ray controls, acute exposures to protons and iron ions disrupts intestinal function and induces mucosal lesions, vascular congestion, epithelial barrier breakdown, and marked enlargement of mucosa-associated lymphoid tissue. We also measured kinetics of DNA double-strand break (DSB) repair using gamma-H2AX- specific antibodies and apoptosis via TUNEL labeling, noting the induction and disappearance of extranuclear cytoplasmic DNA marked by gamma-H2AX only in the charged particle-irradiated samples. We show that 18 h pre-treatment with curcumin-loaded nanolipoprotein particles (cNLPs) delivered via IV injection reduces DSB-associated foci levels and apoptosis and restore crypt villi lengths.Discussion: These data improve our understanding of physiological alterations in the GI tract immediately following exposures to modeled space radiations and demonstrates effectiveness of a promising space radiation MCM.
Effect of hematoma volume on the 30-day mortality rate of patients with primary hypertensive brainstem hemorrhage: a retrospective cohort study
Authors: Yu Zhenxing , Zhang Xianbin , Xu Qiming , Zhang Zhipeng , Xia Yu , Li Huaquan , Yu Xiang , Deng Lei , Zhang Long 
Published: May 2023

ObjectiveThe purpose of this study is to investigate the effect of hematoma volume on the 30-Day Mortality Rate of patients with Primary Hypertensive Brainstem Hemorrhage (PHBH).MethodsRetrospective analysis was done on the clinical information of 74 patients who underwent treatment for primary hypertensive brainstem hemorrhage at the Department of Neurosurgery of the 908th Hospital of the Joint Logistic Support Force of the Chinese People's Liberation Army between January 2018 and December 2021. Both univariate and multivariate logistic regression were used to assess clinical signs and risk factors that affect 30-day mortality.Result(s)In the 74 patients with primary hypertensive brainstem hemorrhage included in this investigation, 46 patients died and 28 patients survived. The mortality rate at 30 days was 62.16%. A statistically significant difference was seen (P &lt; 0.001) in the results of the univariate analysis, which suggested that hematoma volume may be a factor affecting the prognosis of patients with hypertensive brainstem hemorrhage. Hematoma volume was further demonstrated to be a risk factor and an independent factor impacting death in patients with brainstem hemorrhage (P &lt; 0.001) by multivariate logistic regression analysis (OR: 2.6, 95% CI: 1.7–3.9, P &lt; 0.001 Crude Model, OR: 3.6, 95% CI: 1.7–7.7, P &lt; 0.001 Multivariate-Adjusted Model). After adjusting for confounding variables such as age, body mass index, sex, history of diabetes mellitus, history of hypertension, admission GCS score, stereotactic aspiration, combined hydrocephalus, admission systolic and diastolic blood pressure, the hematoma volume was revealed to be an independent predictor of 30-day death in patients with brainstem hemorrhage. We discovered by smooth curve fitting that hematoma volume increased in a non-linear manner with 30-day mortality. The 30-day mortality rate did not alter significantly when the hematoma volume was less than 4 ml. When the hematoma volume was greater than 4 ml, the 30-day mortality rate increased rapidly, and when the hematoma volume was 10 ml, the 30-day mortality rate reached the maximum.Conclusion(s)Hematoma volume is an independent factor affecting 30-day mortality in patients with primary hypertensive brainstem hemorrhage. The severe and extensive neurological damage caused by primary hypertensive brainstem hemorrhage is highly unlikely to be fundamentally altered by a single protocol, and new avenues need to be explored scientifically and continuously.
MicroRNA profiling of cerebrospinal fluid from dogs with steroid responsive meningitis-arteritis and meningoencephalitis of unknown origin
Authors: Mármol-Sánchez Emilio , Heidemann Pernille Lindholm , Gredal Hanne , Cirera Susanna 
Published: May 2023

IntroductionNon-infectious inflammatory diseases of the central nervous system in dogs, such as steroid responsive meningitis-arteritis (SRMA) and meningoencephalitis of unknown origin (MUO), represent a common clinical challenge that needs extensive and multimodal work-up to reach a presumptive diagnosis. Both diseases are presumably caused by dysregulations of the immune system, but further research is needed in order to understand the molecular mechanisms behind each disease and to optimize treatment.MethodsBy next-generation sequencing and subsequent quantitative real-time PCR (qPCR) verification, we designed a prospective case–control pilot study to analyze the small RNA profiles of cerebrospinal fluid from dogs suffering from MUO (N = 5), dogs suffering from SRMA (N = 8), and healthy dogs (N = 5) presented for elective euthanasia used as the Control group.ResultsOur results showed an overall enrichment in Y-RNA fragments across all samples, followed by microRNAs (miRNAs) and ribosomal RNAs as the major findings. Additional traces of short RNA reads mapped to long non-coding RNAs and protein-coding genes were also found. From the detected canine miRNAs, miR-21, miR-486, miR-148a, miR-99a, miR-191 and miR-92a were among the most abundant. Dogs with SRMA showed higher differences in miRNA abundance than dogs with MUO when compared to healthy dogs, and miR-142-3p was consistently detected as differentially upregulated in both diseases, although at a low concentration. Moreover, miR-405-5p and miR-503-5p showed different profiles between SRMA and MUO dogs. Subsequent qPCR analyses confirmed miR-142-5p, miR-191-5p and miR-92a-3p as significantly upregulated miRNAs in dogs with SRMA and/or MUO.DiscussionCerebrospinal fluid is a challenging biological material to use for profiling miRNAs due to the low content of circulating RNAs. Despite this, we could confirm several miRNAs being differentially abundant when comparing healthy dogs and dogs with MUO and SRMA, respectively. The results of this study indicate a potential role of miRNAs in the underlying molecular mechanisms of these diseases and establish the basis for further studies.
Frailty in Older Adults and Internal and Forced Migration in Urban Neighborhood Contexts in Colombia
Authors: Rengifo-Reina Herney , Barrientos-Gutiérrez Tonatiuh , López-Olmedo Nancy , Sánchez Brisa N. , Diez Roux Ana V. 
Published: May 2023

Objective: We investigated the association between the density of internal human migration, in the urban neighborhood, on frailty in the older adult population in Colombia.Methods: The data used in this study are from four Colombian population surveys. We analyzed 633 census tracts with a sample of 2,194 adults 60 years and over for frailty (measured using the Fried criteria). We considered the proportion of inhabitants in a census tract with a history of internal migration as the exposure variable considering three temporalities. For contextual forced migration, we identified two types: 5-year, and 1-year. Poisson multivariable regression models with two hierarchical levels (individual and census tracts) were estimated.Results: The prevalence of pre-fragile/frailty was 80.63% [CI 95%: 77.67, 83.28]. The prevalence ratio were significantly higher for the older adults who live in neighborhoods where a higher proportion of internal migrants reside.Conclusion: We conclude that older adults who lived in neighborhoods with a high proportion of internal migrants experience more frailty. Potential explanations are that neighborhoods with high internal migration could experience social (l increase in cultural heterogeneity, in the perception of insecurity, violence and physical conditions (pressure on local economies and services, leading elderly residents to compete for neighborhood resources), translated into social stress.
Clinical Features and Outcomes of Enterococcal Bone and Joint Infections and Factors Associated with Treatment Failure over a 13-Year Period in a French Teaching Hospital
Authors: Martin Aurélie , Loubet Paul , Salipante Florian , Laffont-Lozes Paul , Mazet Julien , Lavigne Jean-Philippe , Cellier Nicolas , Sotto Albert , Larcher Romaric 
Published: May 2023

Enterococcal bone and joint infections (BJIs) are reported to have poor outcomes, but there are conflicting results. This study aimed to describe the clinical characteristics and outcomes of patients with enterococcal BJI and to assess the factors associated with treatment failure. We conducted a retrospective cohort study at Nimes University Hospital from January 2007 to December 2020. The factors associated with treatment failure were assessed using a Cox model. We included 90 consecutive adult patients, 11 with native BJIs, 40 with prosthetic joint infections and 39 with orthopedic implant-associated infections. Two-thirds of patients had local signs of infection, but few (9%) had fever. Most BJIs were caused by Enterococcus faecalis (n = 82, 91%) and were polymicrobial (n = 75, 83%). The treatment failure rate was 39%, and treatment failure was associated with coinfection with Staphylococcus epidermidis (adjusted hazard ratio = 3.04, confidence interval at 95% [1.31–7.07], p = 0.01) and with the presence of local signs of inflammation at the time of diagnosis (aHR = 2.39, CI 95% [1.22–4.69], p = 0.01). Our results confirm the poor prognosis of enterococcal BJIs, prompting clinicians to carefully monitor for local signs of infection and to optimize the medical-surgical management in case of coinfections, especially with S. epidermidis.
Проблеми метрологічної діяльності у сфері охорони здоров’я
Authors: Чижик І. В. , Сушко Л. Г. 
Published: May 2023

Розглядаються питання, пов’язані із забезпеченням єдності вимірювань у сфері охорони здоров’я, відповідності метрологічної діяльності у зазначеній сфері вимогам чинного законодавства у сфері метрології.
Розглядаються вимоги керівних документів Міністерства охорони здоров’я України, що регламентують шляхи впровадження системи менеджменту якості та забезпечення єдності вимірювань в медичних лабораторіях, та стан їх впровадження.
Зазначаються проблеми, пов’язані з методичним забезпеченням лабораторних досліджень у сфері охорони здоров’я, відсутністю метрологічних вимог до процедур (методик) лабораторних досліджень, метрологічним підтверджуванням засобів вимірювальної техніки медичного призначення.
В статті також зазначаються проблеми, пов’язані з акредитацією медичних лабораторій відповідно до вимог ДСТУ EN ISO 15189 [1].
Reação anafilática e outras complicações após administração da ondansetrona: um relato de caso
Authors: De Almeida Laisy Amorim Farias , Lemos Renata Ferreira , Pontes Thiago Araujo , De Andrade Kelly Cristina Lira , Do Nascimento Graciliano Ramos Alencar 
Published: May 2023

A ondansetrona é um fármaco antagonista do receptor 5-HT3 de serotonina precrito para o tratamento de náuseas e êmese. O medicamento apresenta como reações adversas comuns a cefaléia e constipação, como reações muito raras o broncoespasmo e a anafilaxia. A anafilaxia trata-se de uma reação que geralmente ocorre quando associada a drogas causadoras de bradicardia. É descrito o caso de um paciente, sexo feminino, 40 anos, previamente hígida, a qual procurou o pronto atendimento com a queixa de tontura e mal estar, onde foi administrada a droga Cloridrato de Ondansetrona 8mg intravenosa e a paciente apresentou reação anafilática, convulsão e parada cardiorespiratória. A discussão deste trabalho, apesar de tratar de um evento adverso raro, é fundamental que seja considerada pela comunidade médica tendo em vista o amplo uso da droga e seus possíveis desfeichos graves no atendimento aos pacientes.
Síndrome de Burnout em docentes: fatores de risco e métodos para prevenção
Authors: Bergamini Raiane dos Santos , Amaral Pâmella Polastry Braga , Bergamini Robert dos Santos , Santos Caio Macueidi , Dos Reis Vanessa Oliveira , Marson Renan Fava , De Sousa Marlei Novaes , Antonucci Gabrielli , Alves Carolina de Oliveira , Da Costa Leonária Fernandes , Sampaio Neto Tarcilio José salles , Ronconi Nagila Mai , Da Silva Patrícia Batista , Feller Alexandre Andreatta 
Published: May 2023

A Síndrome de Burnout tem se mostrado crescente, principalmente em profissionais da educação, onde diferentes causas têm sido remetidas ao surgimento da mesma, como carência de recursos, de suporte e pressões advindas da convivência interpessoal, gerando uma profissão de suma desenvoltura e estresse. O objetivo desse estudo é analisar através de uma revisão integrativa na literatura os principais fatores de riscos e estratégias para prevenção da SB em docentes. Trata-se de uma revisão integrativa da literatura proveniente da BVS com bases de dados da Scielo, LILACS e MEDLINE, através dos seguintes DeCS: Síndrome de Burnout”, “Docência” e “Fatores associados”, combinados entre si pelo operador booleano AND. Foram encontrados 128 estudos e selecionados 8 artigos para compor esta revisão. Os principais fatores associados a SB descritas nos estudos foram as relações interpessoais, a falta de estrutura e ambiente adequados para o desenvolvimento do ensino, desinteresse por parte dos alunos, relação de desgaste com superiores e demais fatores como violência, assédio e questões relacionadas as necessidades humanas básicas, além de questões salariais, a desvalorização profissional e a alta jornada de trabalho. Os achados reforçam necessidade de aproximar políticas de saúde do trabalhador voltadas ao ambiente escolar/acadêmico, sobretudo na prevenção de causas de transtornos mentais relacionadas ao desenvolvimento da SB nesses profissionais.
Projeto terapêutico singular na assistência hospitalar: uma revisão integrativa da literatura
Authors: Gamarra Caren Renata , Macário Irma 
Published: May 2023

O Projeto Terapêutico Singular consiste na elaboração de cuidados únicos e individuais a sujeitos que vivenciam alguma situação de adoecimento, sendo uma ferramenta importante para o sucesso do Sistema Único de Saúde brasileiro. Tal projeto é pensado para aplicar-se em todos os níveis de atenção à saúde, entretanto, sabe-se que a atenção hospitalar possui suas peculiaridades em relação às demais. Dessa forma, objetivou-se realizar levantamento de pesquisas que abordassem a temática da utilização do Projeto Terapêutico Singular em unidade hospitalar. A pesquisa consiste numa Revisão Sistemática da Literatura com pesquisa as bases de dados Scielo, BVSaúde e PubMed de artigos na língua portuguesa e inglesa através do uso das palavras-chave e utilização de critérios de inclusão e exclusão. Observou-se a presença de poucos estudos, sendo estes com metodologias qualitativas. Ademais, tais pesquisas apresentaram resultados semelhantes como a presença de maior propensão à humanização, integralidade do cuidado, produção e manutenção e favorecimento da atuação interdisciplinar. Além disso encontrou-se desafios para a realização do projeto relacionados à obstáculos da rotina hospitalar, como recursos materiais e imateriais.
Índice de capacidade para o trabalho de enfermeiros atuantes em áreas críticas Covid-19: estudo transversal
Authors: Garcia Izabela Melo , Haddad Maria do Carmo Fernandez Lourenço , Malaquias Tatiana da Silva Melo , De Oliveira João Lucas Campos , Aroni Patricia 
Published: May 2023

Objetivo: mensurar o índice de capacidade para o trabalho de enfermeiros nos diferentes turnos de um hospital universitário público com atendimento a pacientes com suspeita e confirmados com COVID-19. Método: estudo transversal realizado em hospital público de alta complexidade. A população do estudo foi constituída por enfermeiros atuantes no Pronto Socorro e Unidade de Terapia Intensiva Adulto e os resultados obtidos foram analisados por estatística descritiva. Resultados: dos profissionais entrevistados, 85,5% eram mulheres; a idade média foi de 36,5 anos. Quanto a classificação de capacidade para o trabalho 7,3% foram classificados com ótima capacidade para o trabalho, 54,5% boa capacidade para o trabalho; 34,5% moderada capacidade para o trabalho; e 3,6% baixa capacidade. Conclusão: o Índice de Capacidade para o Trabalho dos colaboradores permite aos gestores a manutenção da boa capacidade para o trabalho, além de propor medidas de melhorias e promover um ambiente com condições de trabalho mais favoráveis.
Enzyme Commission Number Prediction and Benchmarking with Hierarchical Dual-core Multitask Learning Framework
Authors: Shi Zhenkun , Deng Rui , Yuan Qianqian , Mao Zhitao , Wang Ruoyu , Li Haoran , Liao Xiaoping , Ma Hongwu 
Published: May 2023

Enzyme commission (EC) numbers, which associate a protein sequence with the biochemical reactions it catalyzes, are essential for the accurate understanding of enzyme functions and cellular metabolism. Many ab initio computational approaches were proposed to predict EC numbers for given input protein sequences. However, the prediction performance (accuracy, recall, and precision), usability, and efficiency of existing methods decreased seriously when dealing with recently discovered proteins, thus still having much room to be improved. Here, we report HDMLF, a hierarchical dual-core multitask learning framework for accurately predicting EC numbers based on novel deep learning techniques. HDMLF is composed of an embedding core and a learning core; the embedding core adopts the latest protein language model for protein sequence embedding, and the learning core conducts the EC number prediction. Specifically, HDMLF is designed on the basis of a gated recurrent unit framework to perform EC number prediction in the multi-objective hierarchy, multitasking manner. Additionally, we introduced an attention layer to optimize the EC prediction and employed a greedy strategy to integrate and fine-tune the final model. Comparative analyses against 4 representative methods demonstrate that HDMLF stably delivers the highest performance, which improves accuracy and F1 score by 60% and 40% over the state of the art, respectively. An additional case study of tyrB predicted to compensate for the loss of aspartate aminotransferase aspC, as reported in a previous experimental study, shows that our model can also be used to uncover the enzyme promiscuity. Finally, we established a web platform, namely, ECRECer (
https://ecrecer.biodesign.ac.cn
), using an entirely could-based serverless architecture and provided an offline bundle to improve usability.
Criminalisation of Specific Forms of Online Communication: a South African Legal Perspective
Authors: Watney Murdoch 
Published: May 2023

One of the key findings of the South African Social Media Landscape 2022 study is that the growth in Internet access has, in turn, spurred the use of social media, leading to an estimated 48.8% (28 million) of South Africans being active on social media platforms, such as Facebook, Twitter, Instagram, YouTube, LinkedIn and TikTok. The down side is that online communication may not only become “viral” but it may also be harmful.

Initially Internet regulation focussed primarily on the criminalisation of unlawful online conduct, referred to as a cybercrime, which includes hacking, DDoS attacks, but now the focus has been extended to the criminalisation of specific forms of online communication, referred to as communication offences, which include postings on Facebook or WhatsApp.

The discussion deals with the criminalisation of specific forms of online communication in South Africa, the enforcement of communication offences with reference to the investigative powers of the police, with assistance from service providers, and the manner in which a balance between free speech and security is ensured. It does not deal specifically with the protection of users against harmful content, although a possible consequence of the criminalisation of communication is protection against these forms of communication.
Communication agencies and social media as a communication strategy – Portuguese case study
Authors: Loureiro Marlene 
Published: May 2023

The topic "communication agencies and social media as a communication strategy" arose from the need to understand how digital platforms, namely social media, are used as a communication strategy in the business world. Nowadays, organisations are increasingly aware of the importance of digital platforms and social media in the communication of their products and services, as well as their brand image. The online communication of an organisation has to be coordinated, the different channels and tools have to be in harmony and the message and image have to articulate with each other. Thus, the research question "What is the importance of digital platforms and social media as a communication strategy? The research objectives are: (1) to understand the importance of using digital platforms and social media as a communication strategy for organisations; (2) to analyse the management and production of digital content by a communication agency; (3) to identify what type of company requests a communication strategy; (4) to analyse the change in communication strategies after COVID-19. Methodologically, this case study will describe the communication strategy of a communication agency, using digital platforms and social media, and how it applies it in the communication management of client companies. This analysis will be enriched by an interview with the CEO and Creative Director of the communication agency under study. The results reiterate that companies are increasingly investing in communication through digital platforms, with the aim of reaching the consumer faster and more easily. Through this research it was possible to find out that digital platforms have a great importance in the companies' communication strategy, being fundamental for their dissemination and contact with the potential consumer.