bluuebunny/crossref_metadata_2025_split huggingface dataset:
PDIA4, a new endoplasmic reticulum stress protein, modulates insulin resistance and inflammation in skeletal muscle
Authors: Lee Chien-Hsing , Chiang Chi-Fu , Lin Fu-Huang , Kuo Feng-Chih , Su Sheng-Chiang , Huang Chia-Luen , Li Peng-Fei , Liu Jhih-Syuan , Lu Chieh-Hua , Hsieh Chang-Hsun , Hung Yi-Jen , Shieh Yi-Shing 
Published: December 2022

IntroductionEndoplasmic reticulum (ER) stress has emerged as a key player in insulin resistance (IR) progression in skeletal muscle. Recent reports revealed that ER stress-induced the expression of protein disulfide isomerase family a member 4 (PDIA4), which may be involved in IR-related diseases. A previous study showed that metformin modulated ER stress-induced IR. However, it remained unclear whether metformin alleviated IR by regulating PDIA4 expression in skeletal muscle.MethodsHerein, we used palmitate-induced IR in C2C12 cells and a high-fat diet-induced IR mouse model to document the relations between metformin, IR, and PDIA4.ResultsIn C2C12 cells, palmitate-induced IR increased inflammatory cytokines and PDIA4 expression. Besides, knocking down PDIA4 decreased palmitate-induced IR and inflammation in C2C12 cells. Furthermore, metformin modulated PDIA4 expression and alleviated IR both in vitro and in vivo. In addition, serum PDIA4 concentrations are associated with IR and inflammatory cytokines levels in human subjects.DiscussionThus, this study is the first to demonstrate that PDIA4 participates in the metformin-induced effects on skeletal muscle IR and indicates that PDIA4 is a potential novel therapeutic target for directly alleviating IR.
Influence of Hashimoto thyroiditis on diagnosis and treatment of thyroid nodules
Authors: Mao Linfeng , Zheng Chunmei , Ou Shengzhao , He Youwu , Liao Chuanjie , Deng Ganlu 
Published: December 2022

BackgroundAs the prevalence of Hashimoto’s thyroiditis (HT) and thyroid cancer (TC) has been increasing dramatically in recent years, the association between the two diseases has been widely debated and studied. However, no consistent findings are available and the relationship remains controversial. In this study, we analyzed the influence of HT on the diagnosis and treatment of thyroid nodules and investigated the relationship between HT and TC.MethodsFrom Jan 2017 to Apr 2021, 4678 patients underwent thyroid surgery were collected. Of these patients, 440 were diagnosed with HT (242 nodular goiter (NG) with HT, 198 TC with HT). These patients were grouped when appropriate and the data from these patients were statistically analyzed by using SPSS and GraphPad Prism 6.ResultsHT occurred in 198 of 1089 (18.2%) TC patients and 242 of 3589 (6.74%) patients without TC (p=0.000). In terms of the ultrasonography features, in the NG with HT group, 33.1% (80/242) of patients had fine calcification and 45.9% (111/242) of patients whose TI-RADS classification were &gt; Level 3. In the isolated PTC group, 32.3% (2343/7260) LN were metastasis-positive while in the NG with HT group, only 26.0% (504/1939) LN were metastasis-positive (P=0.000). The proportion of PTMC was significantly higher (P=0.000), while the proportion of multifocal carcinoma was significantly lower when coexisting with HT (P=0.029). When comparing the data from the two groups diagnosed as PTMC coexisting with HT or not, there was no significant difference in the composition ratio of tumor number, LN metastasis, LN dissection area, regional LN metastasis and number of negative/positive LN (P=0.614, P=0.051, P=0.139, P=0.350, P=1.000 and P=0.333 respectively). In the MPTC group, 42.2% (872/2065) LN were metastasis-positive while in the MPTC with HT group, only 23.6% (50/212) LN were metastasis-positive (P=0.000).ConclusionsOur data suggested that HT is associated with an increased risk of developing TC but may be a protective factor against PTC progression and metastasis. The coexistence of HT affects the judgment of thyroid nodules by ultrasonography.
A rare pituitary tumor associated with hyperthyroidism and acromegaly
Authors: Li Li , Meng Liheng , Liu Yuping , Tamrakar Rashi , Yang Xi , Liang Xinghuang , Zhou Jia , Xian Jing , Huang Zhenxing , Qin Yingfen 
Published: December 2022

BackgroundMixed pituitary TSH/GH adenomas are rare adenomas associated with acromegaly and/or thyrotoxicosis, with or without varying degrees of goiter. In this report, we show a case of pituitary adenoma producing both GH and TSH simultaneously.Case presentationA 27-year-old man was diagnosed with pituitary adenoma based on various symptoms and clinical findings. For further examination and treatment, he was hospitalized in our institution. It was likely that this subject had pituitary adenoma producing both GH and TSH. In brain magnetic resonance imaging, there was a giant tumor in the sellar region. After the diagnosis of mixed pituitary TSH/GH adenoma, he was treated with octreotide, then underwent tumor resection, and then received hydrocortisone acetate and levothyroxine sodium. After then, GH and IGF-1 levels were suppressed and thyroid function was normalized. Postoperative immunohistochemistry reports showed GH (+) but TSH (-), which may be insensitive to the antibody used to detect TSH or combined with other factors.ConclusionsThe diagnosis of mixed pituitary TSH/GH adenoma must be combined with clinical manifestations, immunohistochemical staining and relevant hormone levels, and genetic testing if necessary for comprehensive judgment. For patients with large adenomas, it is recommended to use somatostatin analogs to restore TH levels and control the excessive secretion of GH levels before surgery.
Soil characteristics and bare ground cover differ among jurisdictions and disturbance histories in Western US protected area-centered ecosystems
Authors: Aslan Clare E. , Zachmann Luke , Epanchin-Niell Rebecca S. , Brunson Mark W. , Veloz Samuel , Sikes Benjamin A. 
Published: December 2022

IntroductionEcological conditions at a given site are driven by factors including resource availability, habitat connectivity, and disturbance history. Land managers can influence disturbance history at a site by harvesting resources, creating transportation pathways, introducing new species, and altering the frequency and severity of events such as fires and floods. As a result, locations with different land management histories have also likely experienced different disturbance trajectories that, over time, are likely to result in different ecological characteristics.MethodsTo understand how the presence of different management histories may shape ecological conditions across large landscapes, we examined plant and soil characteristics at matched sampling points across jurisdictional boundaries within four Protected Area-Centered Ecosystems (PACEs) in the western US. We employed Bayesian modeling to explore 1) the extent to which specific ecological variables are linked to disturbance and jurisdiction both among and within individual PACEs, and 2) whether disturbance evidence differs among jurisdictions within each PACE.ResultsAcross all jurisdictions we found that disturbances were associated with ecologically meaningful shifts in percent cover of bare ground, forbs, grass, shrubs, and trees, as well as in tree species richness, soil stability, and total carbon. However, the magnitude of shifts varied by PACE. Within PACEs, there were also meaningful associations between some ecological variables and jurisdiction type; the most consistent of these were in soil stability and soil carbon:nitrogen ratios. Disturbance evidence within each PACE was relatively similar across jurisdictions, with strong differences detected between contrast jurisdictions only for the Lassen Volcanic National Park PACE (LAVO).DiscussionThese findings suggest an interaction between management history and geography, such that ecotones appear to manifest at jurisdictional boundaries within some, but not all, contexts of disturbance and location. Additionally, we detected numerous differences between PACEs in the size of disturbance effects on ecological variables, suggesting that while the interplay between disturbance and management explored here appears influential, there remains a large amount of unexplained variance in these landscapes. As continued global change elevates the importance of large landscape habitat connectivity, unaligned management activities among neighboring jurisdictions are likely to influence existing ecological conditions and connectivity, conservation planning, and desired outcomes.
Efficient hyperactive piggyBac transgenesis in Plodia pantry moths
Authors: Heryanto Christa , Mazo-Vargas Anyi , Martin Arnaud 
Published: December 2022

While piggyBac transposon-based transgenesis is widely used in various emerging model organisms, its relatively low transposition rate in butterflies and moths has hindered its use for routine genetic transformation in Lepidoptera. Here, we tested the suitability of a codon-optimized hyperactive piggyBac transposase (hyPBase) in mRNA form to deliver and integrate transgenic cassettes into the genome of the pantry moth Plodia interpunctella. Co-injection of hyPBase mRNA with donor plasmids successfully integrated 1.5–4.4 kb expression cassettes driving the fluorescent markers EGFP, DsRed, or EYFP in eyes and glia with the 3xP3 promoter. Somatic integration and expression of the transgene in the G0 injected generation was detectable from 72-h embryos and onward in larvae, pupae and adults carrying a recessive white-eyed mutation. Overall, 2.5% of injected eggs survived into transgene-bearing adults with mosaic fluorescence. Subsequent outcrossing of fluorescent G0 founders transmitted single-insertion copies of 3xP3::EGFP and 3xP3::EYFP and generated stable isogenic lines. Random in-crossing of a small cohort of G0 founders expressing 3xP3::DsRed yielded a stable transgenic line segregating for more than one transgene insertion site. We discuss how hyPBase can be used to generate stable transgenic resources in Plodia and other moths.
High MBL-expressing genotypes are associated with deterioration in renal function in type 2 diabetes
Authors: Dørflinger G. H. , Høyem P. H. , Laugesen E. , Østergaard J. A. , Funck K. L. , Steffensen R. , Poulsen P. L. , Hansen T. K. , Bjerre M. 
Published: December 2022

IntroductionAccumulating evidence support that mannan-binding lectin (MBL) is a promising prognostic biomarker for risk-stratification of diabetic micro- and macrovascular complications. Serum MBL levels are predominately genetically determined and depend on MBL genotype. However, Type 1 diabetes (T1D) is associated with higher MBL serum levels for a given MBL genotype, but it remains unknown if this is also the case for patients with T2D. In this study, we evaluated the impact of MBL genotypes on renal function trajectories serum MBL levels and compared MBL genotypes in newly diagnosed patients with T2D with age- and sex-matched healthy individuals. Furthermore, we evaluated differences in parameters of insulin resistance within MBL genotypes.MethodsIn a cross-sectional study, we included 100 patients who were recently diagnosed with T2D and 100 age- and sex-matched individuals. We measured serum MBL levels, MBL genotype, standard biochemistry, and DEXA, in all participants. A 5-year clinical follow-up study was conducted, followed by 12-year data on follow-up biochemistry and clinical status for the progression to micro- or macroalbuminuria for the patients with T2D.ResultsWe found similar serum MBL levels and distribution of MBL genotypes between T2D patients and healthy individuals. The serum MBL level for a given MBL genotype did not differ between the groups neither at study entry nor at 5-year follow-up. We found that plasma creatinine increased more rapidly in patients with T2D with the high MBL expression genotype than with the medium/low MBL expression genotype over the 12-year follow-up period (p = 0.029). Serum MBL levels did not correlate with diabetes duration nor with HbA1c. Interestingly, serum MBL was inversely correlated with body fat percentage in individuals with high MBL expression genotypes both at study entry (p=0.0005) and 5-years follow-up (p=0.002).DiscussionContrary to T1D, T2D is not per se associated with increased MBL serum level for a given MBL genotype or with diabetes duration. Serum MBL was inversely correlated with body fat percentage, and T2D patients with the high MBL expression genotype presented with deterioration of renal function.
Summertime dissolved oxygen concentration and hypoxia in the Zhejiang coastal area
Authors: Zhang Xiangdong , Wang Zelin , Cai Huiwen , Chai Xiaoping , Tang Jingliang , Zhuo Lifei , Jia Haibo 
Published: December 2022

IntroductionThe Zhejiang coastal area is the most important fishery ground in East China Sea, located south of the Yangtze River Estuary. The previous studies on hypoxia and mechanisms mainly focused on the outer shelf and Yangtze River Estuary, and limited knowledge on the DO depletion and low DO information in this area.MethodsIn this study, the relationships among the DO spatial variation and depletion with nutrients, Chl-a, algal blooms, stratification were analyzed based on the investigation from July to August in 2020.Results and discussionThe results showed that, the DO contents were high in the surface water (4.6–11.8 mg/L) than in the bottom water (3.0-8.4 mg/L) with an increasing trend from nearshore to offshore in the surface layer, but opposite in the bottom. The vertical profile of DO showed that low DO concentrations (≤3.0 mg/L) started from the water depth of 12 m with a depth of 45 m. The low DO area appeared in the northeast and central Zhejiang coastal areas covering an area of 6,000 km2 and 4,000 km2, respectively. Our analysis indicated that the successive algal blooms (Chl-a &gt; 40.0 μg/L) occurred in late June and during the investigation prepared the low DO pool for the hypoxia development. Stratification, generated from upper warm, light, diluted freshwater from the Yangtze River and the deeper cold, heavy, salty Taiwan Warm Current ceased the vertical convection of DO in the surface, and accelerated the hypoxia development. The hypoxia starting time was earlier and the duration was longer in this complicated area. The rising temperature, more frequency of algal blooms by global warming would likely to make this worse. Continued interdisciplinary research are badly needed to get a better view in the future.
Proteomic analysis of Fasciola gigantica excretory and secretory products (FgESPs) co-immunoprecipitated using a time course of infected buffalo sera
Authors: Zheng Mengwei , Jiang Xuelian , Kong Xinping , Guo Yanfeng , Zhang Weiyu , Di Wenda 
Published: December 2022

IntroductionWidespread Fasciola gigantica infection in buffaloes has caused great economic losses in buffalo farming. Studies on F. gigantica excretory and secretory products (FgESP) have highlighted their importance in F. gigantica parasitism and their potential in vaccine development. Identifying FgESP components involved in F. gigantica-buffalo interactions during different periods is important for developing effective strategies against fasciolosis.MethodsBuffaloes were assigned to non-infection (n = 3, as control group) and infection (n = 3) groups. The infection group was orally administrated 250 metacercariae. Sera were collected at 3, 10, and 16 weeks post-infection (wpi) for the non-infection group and at 0 (pre-infection), 1, 3, 6, 8, 10, 13, and 16 wpi for the infection group. FgESP components interacting with sera from the non-infection and infection groups assay were pulled down by co-IP and identified using LC–MS/MS. Interacting FgESP components in infection group were subjected to Kyoto Encyclopedia of Genes and Genomes (KEGG) metabolic pathway and gene ontology (GO) functional annotation to infer their potential functions.Results and discussionProteins of FgESP components identified in the non-infection group at 3, 10, and 16 wpi accounted for 80.5%, 84.3%, and 82.1% of all proteins identified in these three time points, respectively, indicating surroundings did not affect buffalo immune response during maintenance. Four hundred and ninety proteins were identified in the infection group, of which 87 were consistently identified at 7 time points. Following GO analysis showed that most of these 87 proteins were in biological processes, while KEGG analysis showed they mainly functioned in metabolism and cellular processing, some of which were thought to functions throughout the infection process. The numbers of specific interactors identified for each week were 1 (n = 12), 3 (n = 5), 6 (n = 8), 8 (n = 15), 10 (n = 23), 13 (n = 22), and 16 (n = 14) wpi, some of which were thought to functions in specific infection process. This study screened the antigenic targets in FgESP during a dense time course over a long period. These findings may enhance the understanding of molecular F. gigantica-buffalo interactions and help identify new potential vaccine and drug target candidates.
Identification of miRNA–mRNA regulatory network associated with the glutamatergic system in post-traumatic epilepsy rats
Authors: Zhang Xiaoyuan , Ma Yixun , Zhou Fengjuan , Zhang Mengzhou , Zhao Dong , Wang Xu , Yang Tiantong , Ma Jun 
Published: December 2022

BackgroundGlutamate is one of the most important excitatory neurotransmitters in the mammalian brain and is involved in a variety of neurological disorders. Increasing evidence also shows that microRNA (miRNA) and mRNA pairs are engaged in a variety of pathophysiological processes. However, the miRNA and mRNA pairs that affect the glutamatergic system in post-traumatic epilepsy (PTE) remain unknown.MethodsPTE rats were induced by injecting 0.1 mol/L, 1 μL/min FeCl2 solution. Behavioral scores and EEG monitoring were used to evaluate whether PTE was successfully induced. RNA-seq was used to obtain mRNA and miRNA expression profiles. Bioinformatics analysis was performed to screen differentially expressed mRNAs and miRNAs associated with the glutamatergic system and then predict miRNA–mRNA interaction pairs. Real-time quantitative reverse transcription PCR was used to further validate the expression of the differential miRNAs and mRNAs. The microRNA–mRNA was subject to the Pearson correlation analysis.ResultsEight of the 91 differentially expressed mRNAs were associated with the glutamatergic system, of which six were upregulated and two were downregulated. Forty miRNAs were significantly differentially expressed, with 14 upregulated and 26 downregulated genes. The predicted miRNA–mRNA interaction network shows that five of the eight differentially expressed mRNAs associated with the glutamatergic system were targeted by multiple miRNAs, including Slc17a6, Mef2c, Fyn, Slc25a22, and Shank2, while the remaining three mRNAs were not targeted by any miRNAs. Of the 40 differentially expressed miRNAs, seven miRNAs were found to have multiple target mRNAs associated with the glutamatergic system. Real-time quantitative reverse transcription PCR validation and Pearson correlation analysis were performed on these seven targeted miRNAs—Slc17a6, Mef2c, Fyn, Slc25a22, and Shank2—and six additional miRNAs selected from the literature. Real-time quantitative reverse transcription PCR showed that the expression levels of the mRNAs and miRNAs agreed with the predictions in the study. Among them, the miR-98-5p–Slc17a6, miR-335-5p–Slc17a6, miR-30e-5p–Slc17a6, miR-1224–Slc25a22, and miR-211-5p–Slc25a22 pairs were verified to have negative correlations.ConclusionsOur results indicate that miRNA–mRNA interaction pairs associated with the glutamatergic system are involved in the development of PTE and have potential as diagnostic biomarkers and therapeutic targets for PTE.
Overexpression of Mena is associated with tumor progression and poor prognosis in oral squamous cell carcinoma via EMT
Authors: Na Sijia , Cui Hao , Guo Zhichen , Liang Xiang , Sakran Karim Ahmed , Guo Xiaomei , Li Xingqiang , Xie Linyang , Zhu Yifei , Qi Hong , Tu Junbo 
Published: December 2022

BackgroundMena, a cytoskeletal regulatory protein, is involved in actin-based regulation of cell motility and adhesion, and contributes to tumor invasion and metastasis. However, the role of Mena in oral squamous cell carcinoma remains unclear. This is the first research focusing on the prognostic value of Mena in OSCC. In this study, we aimed to investigate the correlation between Mena expression and clinicopathological significance, as well as prognostic value in OSCC.MethodsMena gene expression profiles of OSCC and normal tissues were collected from Oncomine, TCGA, and GEO databases. Biological function was analyzed through GO, KEGG and GSEA enrichment. Further, the expression level of Mena and tumor-related markers in 151 OSCC specimens was examined by IHC staining based on tissue microarray. Kaplan-Meier analysis was used to assess the prognostic performance of Mena in OSCC.ResultMena was generally upregulation in various malignancies, especially OSCC. The functional analyses indicated that Mena was involved in the assembly and regulation of actin, cell movement, and EMT. IHC staining revealed that high expression of Mena in OSCC was correlated with Lymphatic metastasis, TNM stage, E-cadherin, Vimentin, and MMP-2, but insignificantly Ki67. Kaplan-Meier analysis demonstrated that elevated expression of Mena was significantly associated with poor overall survival and disease-free survival of OSCC patients.ConclusionMena could be a novel biomarker for predicting the prognosis of OSCC patients, which supports a theoretical basis for developing molecular target therapy.
Case report: Gemcitabine intravesical hyperthermic infusion combined with tislelizumab in muscle invasive bladder urothelium carcinoma
Authors: Du Zheng , Yin Huaqi , Zhao Shiming , Ma Yongkang , Sun Zhenghui , Dong Bingqi , Zhu Mingkai , Zhu Chaoshuai , Peng Jiangshan , Yang Tiejun 
Published: December 2022

BackgroundMuscle invasive bladder urothelium carcinoma is a common urinary tract tumor. With the deepening of research, more and more treatment methods are applied in clinical practice, extending the life of patients. Among them, the clinical application of chemotherapeutic intravesical hyperthermia and tumor immunotherapy provides new ideas for our treatment.Case reportAn 81-year-old female patient was diagnosed with stage T2N0M0 bladder cancer in our hospital. Because the patient and her family were keen to preserve her bladder, they declined surgery and opted for combined chemotherapy. After informed consent from the patient and her family, she received cisplatin combined with gemcitabine intravesical hyperthermic infusion. But the side effects of cisplatin made her intolerable to chemotherapy. With their informed consent we changed her to intravenous tislelizumab in combination with gemcitabine intravesical hyperthermic infusion to continue her treatment. During the subsequent follow-up visits, we found a surprising effect of the treatment.ConclusionGemcitabine intravesical hyperthermia therapy combined with intravenous tislelizumab in the treatment of muscle invasive bladder urothelium carcinoma may provide a new possible therapeutic strategy of some patients who are inoperable or refuse surgery.