bluuebunny/crossref_metadata_2025_split huggingface dataset:
Curcumin suppressed the proliferation and apoptosis of HPV‐positive cervical cancer cells by directly targeting the E6 protein
Authors: Zhao Xingyu , Zhang Ruowen , Song Zitong , Yang Kun , He Han , Jin Lianhai , Zhang Wei 
Published: May 2023

AbstractMost human papillomavirus (HPV) types, including HPV16 and HPV18, are closely related to the occurrence of cervical cancer, predominantly through the action of viral oncoproteins E6 and E7. Curcumin, the active ingredient of the turmeric plant, has been gaining attention over the past two decades as an antioxidant, anti‐inflammatory, and anticancer agent. In the present study, the HPV‐positive cervical cancer cells HeLa and CaSki were treated with curcumin, and the results showed that curcumin has a dose‐dependent and time‐dependent inhibitory effect on cell viability. In addition, apoptosis induction was further quantitatively confirmed through flow cytometric analysis. Furthermore, the influence of different concentrations of curcumin on the mitochondrial membrane potential was evaluated through JC‐1 staining and found to dramatically decrease the membrane potential in treated HeLa and CaSki cells, suggesting the critical role of the mitochondrial pathway in their apoptosis‐inducing effect. This study also demonstrated the wound‐healing potential of curcumin, and the results of transwell assays showed that curcumin treatment inhibited HeLa and CaSki cell invasion and migration in a dose‐dependent manner compared with the control treatment. Curcumin also downregulated the expression of Bcl‐2, N‐cadherin, and Vimentin and upregulated the expression of Bax, C‐caspase‐3, and E‐cadherin in both cell lines. Further research showed that curcumin also selectively inhibited the expression of the viral oncoproteins E6 and E7, as demonstrated by western blot analysis; moreover, the downregulation of E6 was more significant than that of E7. Our research also showed that coculture with cells infected with siE6 lentivirus (siE6 cells) can inhibit the proliferation, invasion, and metastasis of HPV‐positive cells. While the siE6 cells were also treated with curcumin, the effect of curcumin monotherapy was offset. In summary, our research shows that curcumin regulates the apoptosis, migration, and invasion of cervical cancer cells, and the mechanism may be related to its ability to downregulate E6. This study provides a foundation for future research on the prevention and treatment of cervical cancer.
LC‐HRMS‐based metabolomics workflow: An alternative strategy for metabolite identification in the antidoping field
Authors: Leogrande Patrizia , Jartdines Daniel , Martinez‐Brito Dayamin , de la Torre Xavier , Parr Maria Kristina , Botrè Francesco 
Published: May 2023

RationaleThe proposed metabolomic workflow, based on coupling high‐resolution mass spectrometry with computational tools, can be an alternative strategy for metabolite detection and identification. This approach allows the extension of the investigation field to chemically different compounds, maximizing the information obtainable from the data and minimizing the time and resources required.MethodsUrine samples were collected from five healthy volunteers before and after oral administration of 3β‐hydroxyandrost‐5‐ene‐7,17‐dione as a model compound and defining three excretion time intervals. Raw data were acquired in both positive and negative ionization modes using an Agilent Technologies 1290 Infinity II series HPLC coupled to a 6545 Accurate‐Mass Quadrupole Time‐of‐Flight. They were then processed to align peak retention times with the same accurate mass, and the resulting data matrix was subjected to multivariate analysis.ResultsMultivariate analysis (PCA and PLS‐DA models) demonstrated high similarity between samples belonging to the same collection time interval and clear discrimination between different excretion intervals. The blank and long excretion groups were distinguished, suggesting the presence of long excretion markers, which are of remarkable interest in anti‐doping analyses. The correspondence of some significant features with metabolites reported in the literature confirmed the rationale and usefulness of the proposed metabolomic approach.ConclusionsThe presented study proposes a metabolomics workflow for the early detection and characterization of drug metabolites by untargeted urinary analysis to reduce the range of substances still excluded from routine screening. Its application has detected minor steroid metabolites, as well as unexpected endogenous alterations, proving to be an alternative strategy that can allow gathering a more complete range of information in the antidoping field.
N‐Terminal chemical derivatization of peptides with 4‐formyl‐benzenesulfonic acid and electrospray positive and negative tandem mass spectrometry with high abundance of b‐ion series
Authors: Ozdanovac Luka , Dončević Lucija , Hozić Amela , Biba Renata , Svetličić Ema , Janeš Andrea , Cindrić Mario 
Published: May 2023

RationaleSelective derivatization of peptide N‐terminus with 4‐formyl‐benzenesulfonic acid (FBSA) enables chemically activated fragmentation in positive and negative ion modes (ESI+/−) under charge reduction conditions. Overlapped positive and negative tandem mass spectra show b‐ions making the assignment of b‐ion series fragments easy and accurate.MethodsWe developed an FBSA–peptide microwave‐assisted derivatization procedure. Derivatized and nonderivatized bovine serum albumin tryptic peptides and insulin non‐tryptic peptide were compared after tandem mass spectrometry (MS/MS) analysis in positive and negative ion modes. A high‐quality data set of sulfonated b‐ions obtained in negative tandem mass spectra of singly charged FBSA–peptides were matched to detected b‐ions in positive MS/MS spectra. Moreover, negative spectra signals were converted and matched against y‐ions in positive tandem mass spectra to identify complete peptide sequences.ResultsThe FBSA derivatization procedure produced a significantly improved MS/MS data set (populated by high‐intensity signals of b‐ and y‐ions) compared to commonly used N‐terminal sulfonation reagents. Undesired side reactions almost do not occur, and the procedure reduces the derivatization time. It was found that b‐ion intensities comprise 15% and 13% compared to combined ion intensities generated in positive‐ and negative ion modes, respectively. High visibility of b‐ion series in negative ion mode can be attributed to N‐terminal sulfonation that had no negative effect on the production of b‐ and y‐ion series in positive ion mode.ConclusionsThe FBSA derivatization and de novo sequencing approach outlined here is a reliable method for accurate peptide sequence assignment. Increased production of b‐ions in positive‐ and negative ion modes greatly improves peak assignment and thus enables accurate sequence reconstruction. Implementation of the named methodology would improve the quality of de novo sequencing data and reduce the number of misinterpreted spectra.
Cooperative output feedback tracking control of stochastic nonlinear heterogeneous multi‐agent systems
Authors: Li Dianqiang , Li Tao 
Published: May 2023

SummaryWe study cooperative output feedback tracking control of stochastic nonlinear heterogeneous leader‐following multi‐agent systems. Each agent has a continuous‐time stochastic nonlinear heterogeneous dynamics with an unmeasurable state, and there are additive and multiplicative noises along with information exchange among agents. We propose admissible distributed observation strategies for estimating the leader's and the followers' states, and admissible cooperative output feedback control strategies based on the certainty equivalence principle. By output regulation theory and stochastic analysis, we show if the dynamics of each agent satisfies the Lipschitz condition, and the product of the leader's Lipschitz coefficient, the intensity of multiplicative measurement noises, and the constant related to the leader's Lipschitz coefficient and dimension is less than the minimum nonzero eigenvalue of graph Laplacian, then there exist admissible distributed observation and cooperative control strategies to ensure mean square bounded output tracking. Finally, the effectiveness of our control strategies is demonstrated by a numerical simulation.
Host selection has a stronger impact on leaf microbiome assembly compared to land‐management practices
Authors: Singh Pankaj K. , Egidi Eleonora , Macdonald Catriona A. , Singh Brajesh K. 
Published: May 2023

AbstractIntroductionPlant microbiomes contribute directly to plant health and productivity, but mechanisms that underpin plant microbiome assembly in different compartments (e.g., root, leaf) are not fully understood. Identifying environmental and management factors that affect plant microbiome assembly is important to advance understanding of fundamental ecological processes, and to harness microbiome for improved primary productivity and environmental sustainability. Irrigation and fertilization are two common management practices in Australian tree plantations, but little is known about the effects of these treatments on soil, plant host and their microbiome. Here, we investigated the impact of decade‐long irrigation, fertilization and their combined application on soil, plant traits and microbiome of aEucalyptus salignaplantation.Materials and MethodsMicrobial profiling of bulk soil, rhizosphere, root and leaves was performed using amplicon sequencing 16S ribosomal DNA and internal transcribed spacer (ITS) markers for bacteria and fungi, respectively, along with measurements of soil properties and plant traits.ResultsThe results indicated that both management practices significantly affected soil properties and soil and root microbiomes. Irrigation increased but fertilizer treatment reduced microbial alpha diversity. However, neither irrigation nor fertilizer treatment impacted the leaf microbiome. Our findings suggest that management practices impact soil edaphic factors, which in turn influence the below‐ground microbiome (soil and root), but the leaf microbiome remains unaffected. In addition, the leaf microbiome was distinct from soil and root microbiomes, and a source tracker analysis suggested that root and bulk soils only contributed to 53% and 10% operational taxonomic units of the leaf bacterial community, suggesting strong and sequential host selection of the leaf microbiome. In addition, management practices had a limited impact on leaf traits and, consequently, the leaf microbiome maintained its distinct composition.ConclusionThese findings provide mechanistic evidence for ecological processes that drive plant microbiome assembly and indicate that host selection plays a more important role than management practices in the leaf microbiome assembly.
Super‐Macroporous Lightweight Materials Templated from Bicontinuous Intra‐Phase Jammed Emulsion Gels Based on Nanochitin
Authors: Lu Yi , Kamkar Milad , Guo Shasha , Niu Xun , Wan Zhangmin , Xu Junhua , Su Xiaoya , Fan Yimin , Bai Long , Rojas Orlando J. 
Published: May 2023

AbstractNon‐equilibrium multiphase systems are formed by mixing two immiscible nanoparticle dispersions, leading to bicontinuous emulsions that template cryogels with interconnected, tortuous channels. Herein, a renewable, rod‐like biocolloid (chitin nanocrystals, ChNC) is used to kinetically arrest bicontinuous morphologies. Specifically, it is found that ChNC stabilizes intra‐phase jammed bicontinuous systems at an ultra‐low particle concentration (as low as 0.6 wt.%), leading to tailorable morphologies. The synergistic effects of ChNC high aspect ratio, intrinsic stiffness, and interparticle interactions produce hydrogelation and, upon drying, lead to open channels bearing dual characteristic sizes, suitably integrated into robust bicontinuous ultra‐lightweight solids. Overall, it demonstrates the successful formation of ChNC‐jammed bicontinuous emulsions and a facile emulsion templating route to synthesize chitin cryogels that form unique super‐macroporous networks.
Magnetic Covalent Organic Framework Composites for Wastewater Remediation
Authors: Yang Juan , Huang Lijin , You Jungmok , Yamauchi Yusuke 
Published: May 2023

AbstractCovalent organic frameworks (COFs) with high specific surface area, tailored structure, easy functionalization, and excellent chemical stability have been extensively exploited as fantastic materials in various fields. However, in most cases, COFs prepared in powder form suffer from the disadvantages of tedious operation, strong tendency to agglomerate, and poor recyclability, greatly limiting their practical application in environmental remediation. To tackle these issues, the fabrication of magnetic COFs (MCOFs) has attracted tremendous attention. In this review, several reliable strategies for the fabrication of MCOFs are summarized. In addition, the recent application of MCOFs as outstanding adsorbents for the removal of contaminants including toxic metal ions, dyes, pharmaceuticals and personal care products, and other organic pollutants is discussed. Moreover, in‐depth discussions regarding the structural parameters affecting the practical potential of MCOFs are highlighted in detail. Finally, the current challenges and future prospects of MCOFs in this field are provided with the expectation to boost their practical application.
Low Detection Limit Circularly Polarized Light Detection Realized by Constructing Chiral Perovskite/Si Heterostructures
Authors: Wu Jianbo , Zhang Xinyuan , You Shihai , Zhu Zeng‐Kui , Zhu Tingting , Wang Ziyang , Li Ruiqing , Guan Qianwen , Liang Lishan , Niu Xinyi , Luo Junhua 
Published: May 2023

AbstractChiral perovskites have been demonstrated as promising candidates for direct circularly polarized light (CPL) detection due to their intrinsic chirality and excellent charge transport ability. However, chiral perovskite‐based CPL detectors with both high distinguishability of left‐ and right‐handed optical signals and low detection limit remain unexplored. Here, a heterostructure, (R‐MPA)2MAPb2I7/Si (MPA = methylphenethylamine, MA = methylammonium) is constructed, to achieve high‐sensitive and low‐limit CPL detection. The heterostructures with high crystalline quality and sharp interface exhibit a strong built‐in electric field and a suppressed dark current, not only improving the separation and transport of the photogenerated carriers but also laying a foundation for weak CPL signals detection. Consequently, the heterostructure‐based CPL detector obtains a high anisotropy factor up to 0.34 with a remarkably low CPL detection limit of 890 nW cm−2 under the self‐driven mode. As a pioneering study, this work paves the way for designing high‐sensitive CPL detectors that simultaneously have great distinguishing capability and low detection limit of CPL.
Experimental study on basic and drying creep for an alkali‐activated slag concrete and comparison with existing creep models
Authors: Caron Richard , Patel Ravi A. , Dehn Frank 
Published: May 2023

AbstractSlag is a by‐product of the steel industry that can be activated using alkali solutions to form concrete. This study presents new experimental results of basic and drying creep behavior of alkali‐activated slag (AAS) concrete. Different parameters affecting creep such as loading age, sample size and creep stress‐strength ratio were varied for experimental studies. The results show that the basic creep of AAS concrete is higher than that of ordinary Portland cement (OPC) concrete. The drying creep of AAS is lower than for OPC and this could be explained by a higher internal drying during the activation of slag. The experimental results were used to check the applicability of two existing engineering models, the fib MC 2010 and the B4s model, for AAS concrete. It was found that both models could be extended to predict the basic creep of AAS concrete. For drying creep, the B4s could better capture the creep behavior. For the fib MC 2010, a new formulation for drying creep would be required.
Gossypin exert lipopolysaccharide induced lung inflammation via alteration of Nrf2/HO‐1 and NF‐κB signaling pathway
Authors: Huang Hao , Wang Jian , Hussain Shaik Althaf , Gangireddygari Venkata Subba Reddy , Fan Yingying 
Published: May 2023

AbstractAcute Lung Injury (ALI) is a critical medical condition that induces the injury into the lung tissue, resulting in decreased the oxygen levels in the circulation and finally causes the respiratory failure. In this study, we try to made effort for scrutinized the preventive effect of gossypin against lipopolysaccharide (LPS) induced lung inflammation and explore the underlying mechanism. LPS (7.5 mg/kg) was used for induction the lung inflammation in the rats and rats were received the oral administration of gossypin (5, 10 and 15 mg/kg). The wet to dry weight lung ratio and lung index were estimated. The bronchoalveolar lavage fluid (BALF) were collected to determination the inflammatory cells, total protein, macrophages and neutrophils. ELISA kits were used for the estimation of antioxidant, inflammatory cytokines, inflammatory parameters, nuclear factor erythroid 2‐related factor 2 (Nrf2) and heme oxygenase‐1 (HO‐1) parameters. Finally, we used the lung tissue for scrutinize the alteration in the lung histopathology. Gossypin treatment significantly (p < .001) reduced the W/D ratio of lung tissue and lung index. Gossypin significantly (p < .001) decreased the total cells, neutrophils, macrophages and total protein in BALF. It is also altered the level of inflammatory cytokines, antioxidant and inflammatory parameters, respectively. Gossypin improved the level of Nrf2 and HO‐1 at dose dependent manner. Gossypin treatment remarkably enhance the ALI severity via balancing the structural integrity of lung tissue, decrease the thickness of the alveolar wall, decline the pulmonary interstitial edema, and number of inflammatory cells in the lung tissue. Gossypin is a promising agent for the treatment of LPS induced lung inflammation via altering Nrf2/HO‐1 and NF‐κB.
Hydroquinone‐induced endoplasmic reticulum stress affects TK6 cell autophagy and apoptosis via ATF6‐mTOR
Authors: Li Boxin , Yang Hui , Wu Haipeng , Wang Huanhuan , Ye Zhongming , Wu Yao , Chen Yuting , Tang Huanwen 
Published: May 2023

AbstractHydroquinone (HQ), one of the main active metabolites of benzene in vivo, 7is commonly used as a surrogate for benzene in in vitro studies and has been shown to be cytotoxic. The aim of this study was to investigate the role of endoplasmic reticulum stress (ERS) in HQ‐induced autophagy and apoptosis in human lymphoblastoid cells (TK6) and how activating transcription factor 6 (ATF‐6) is involved. We treated TK6 cells with HQ to establish a cytotoxicity model and found that HQ induced cellular ERS, autophagy and apoptosis by Western blot, flow cytometry and transmission electron microscopy. In addition, inhibition of both reactive oxygen species (ROS) and ERS inhibited cellular autophagy and apoptosis, suggesting that ERS may be induced by ROS, which in turn affects autophagy and apoptosis. Our study also found that HQ could inhibit ATF6 expression and mTOR activation. Knockdown of ATF6 enhanced autophagy and apoptosis levels and further inhibited mTOR activation; activation of ATF6 by AA147 enhanced cellular activity, suggesting that ATF6 may affect cellular autophagy and apoptosis through mTOR. In conclusion, our data suggest that ROS mediated ERS may promote autophagy and apoptosis by inhibiting ATF6‐mTOR pathway after HQ treatment of TK6 cells.
Cadmium accelerates autophagy of osteocytes by inhibiting the PI3K/AKT/mTOR signaling pathway
Authors: Song Ruilong , He Shuangjiang , Cao Ying , Lu Yicheng , Peng Yunwen , Zou Hui , Tong Xishuai , Ran Di , Ma Yonggang , Liu Zongping 
Published: May 2023

AbstractCadmium (Cd) can damage bone cells and cause osteoporosis. Osteocytes are the most numerous bone cells and also important target cells for Cd‐induced osteotoxic damage. Autophagy plays important role in the progression of osteoporosis. However, osteocyte autophagy in Cd‐induced bone injury is not well characterized. Thus, we established a Cd‐induced bone injury model in BALB/c mice and a cellular damage model in MLO‐Y4 cells. Aqueous Cd exposure for 16 months showed an increase in plasma alkaline phosphatase (ALP) activity and increase in urine calcium (Ca) and phosphorus (P) concentrations in vivo. Moreover, expression level of autophagy‐related microtubule‐associated protein 1A/1B‐light chain 3 II (LC3II) and autophagy‐related 5 (ATG5) proteins were induced, and the expression of sequestosome‐1 (p62) was reduced, along with Cd‐induced trabecular bone damage. In addition, Cd inhibited the phosphorylation of mammalian target of rapamycin (mTOR), protein kinase B (AKT), and phosphatidylinositol 3‐kinase (PI3K). In vitro, 80 μM Cd concentrations exposure upregulated LC3II protein expression, and downregulated of p62 protein expression. Similarly, we found that treatment with 80 μM Cd resulted in a reduction in the phosphorylation levels of mTOR, AKT, and PI3K. Further experiments revealed that addition of rapamycin, an autophagy inducer, enhanced autophagy and alleviated the Cd‐induced damage to MLO‐Y4 cells. The findings of our study reveal for the first time that Cd causes damage to both bone and osteocytes, as well as induces autophagy in osteocytes and inhibits PI3K/AKT/mTOR signaling, which could be a protective mechanism against Cd‐induced bone injury.
Extension of the HKLL bulk reconstruction for small ∆
Authors: Aoki Sinya , Balog János 
Published: May 2023

AbstractWe re-analyse the bulk reconstruction for a scalar field in Lorentzian AdS spacetime, both for the case of even and odd dimensions, for an extended range of conformal dimensions where the original HKLL reconstruction has to be modified. We also discuss the use of space-like Green’s functions in the bulk reconstruction. We demonstrate that in the extended range also the singular part of the Green’s function, omitted in the original papers, has be included. The results are particularly simple and physically interesting for integer conformal dimensions below the range considered in the original HKLL papers.
Complete NLO operators in the Higgs effective field theory
Authors: Sun Hao , Xiao Ming-Lei , Yu Jiang-Hao 
Published: May 2023

Abstract
We enumerate the complete and independent sets of operators at the next-to-leading order (NLO) in the Higgs effective field theory (HEFT), based on the Young tensor technique on the Lorentz, gauge, and flavor structures. The operator-amplitude correspondence tells a type of operator forms the on-shell amplitude basis, and for operators involved in Nambu-Goldstone bosons, the amplitude basis is further reduced to the subspace satisfying the Adler zero condition in the soft momentum limit. Different from dynamical fields, the spurion should not enter into the Lorentz sector, instead, it only plays the role of forming the SU(2) invariant together with other dynamical fields. With these new treatments, for the first time, we could obtain the 224 (7704) operators for one (three) generation fermions, 295 (11307) with right-handed neutrinos, and find there were 8 (11) terms of operators missing and many redundant operators can be removed in the effective theory without (with) right-handed neutrinos.