bluuebunny/crossref_metadata_2025_split huggingface dataset:
Apple Pomace as an Alternative Substrate for Butanol Production
Authors: Tigunova Olena , Bratishko Viacheslav , Shulga Sergiy 
Published: September 2023

Abstract
Butanol-producing strains Clostridium sp. UCM B-7570 and C. acetobutylicum UCM B-7407 were used for research from "Collection of strains of microorganisms and plant lines for food and agricultural biotechnology" of the Institute of Food Biotechnology and Genomics of the National Academy of Sciences of Ukraine, glycerol (BASF, Germany) and apple pomace (total moisture 4%) after apple juice production. The aim of this work was to study the possibility of using apple pomace by domestic butanol-producing strains of Clostridium sp. UCM B-7570 and C. acetobutylicum UCM B-7407 as a substrate. Producers were cultured on medium with different concentrations of apple pomace, glycerol was used for the inoculation. The presence of ethanol, acetone, and butanol in the culture liquid was determined using a gas chromatograph. It was determined that a significant part of the macrocomponent composition of the extracts can be used in bioconversion by producing strains of the genus Clostridium. It was determined that the highest concentration of butanol (10 g/dm3) was at a concentration of 120 g/dm3 in the extracts. The obtained data showed the possibility of using apple pomace as a substrate in biobutanol technology.
During a storm – the impact of the COVID-19 pandemic on the epidemiology of the most frequent cancers in Poland
Authors: Choręza Piotr , Owczarek Aleksander Jerzy , Kruk Wacław , Chudek Jerzy 
Published: September 2023

Abstract
Background
The late diagnosis, despite improving availability and accessibility of diagnostic procedures during the last decade in Poland and cooperation between specialist cancer centres, remains an unsolved problem. A new coronavirus SARS-CoV2 causing the COVID-19 disease occurred in the end of 2019. In a few months, it has become the most fundamental problem for public health worldwide. The accessibility to healthcare resources and diagnostic procedures has been drastically reduced because of the COVID-19 pandemic in 2019–2020. The study was aimed to assess the effect of the COVID-19 pandemic and slow cancer burden on the healthcare system of the most frequent cancers in Poland in the period 2015–2021.
Methods
Depersonalized, epidemiological data was obtained from the National Health Found of Poland. The epidemiological analysis was performed and divided into subregions, according to patients’ domicile. For each of the subregions, we have calculated the incidence rate per 100,000 standardized to the ESP 2013. The time points of providing the first healthcare service were considered as the time of cancer diagnosis.
Results
Colorectal cancer has become the leading oncological problem in Poland in recent years and the long-term trend shows a significant increase in the SIR in 2021 compared to 2020. The SIR of the lung cancer decreased in 2015–2019 across the country as well as the burden of kidney cancer has decreased in most of the subregions. The breast and prostate cancers were the most frequent cancer diagnosed in Poland among women and men respectively. Such a situation has remained stable till 2020 when the COVID-19 pandemic has occurred. In 2020, compared to 2019 a visible reduction of SIR of the breast cancer in most of the subregions was found, especially in the eastern areas. In 2020 when the COVID-19 pandemic occurred, a visible reduction of the SIR of C61 in most of the subregions was found.
Conclusions
Our results show a significant decrease in the SIR of the most frequent cancers diagnosed in Poland in 2020 compared to 2019. Subsequently, the excessive increase in 2021 was found, the most likely due to the gradual reduction of epidemic restrictions.
Study on the protein and peptide absorbents for capture and storage of CO2 : Interactions bwteen CO2 and amino acids
Authors: Long Si-Yu , Shen Nai-Kun , Liu Jin-Lei , Zhou Ling-Qiang , Tang Pei-Duo , Du Qi-Shi 
Published: September 2023

Abstract
Interactions between amino acid side chains and CO2 molecules are systematically evaluated using quantum chemical calculations and NMR experiments. The binding energies of CO2 in three proteins (phosphoenolpyruvate carboxykinase, rhodobacter capsulatus, and carbonic anhydrase II) are analyzed based on QM and MD simulations. In the 20 natural amino acids of proteins 13 charged or polar amino acids possess remarkable ability of electrostatic interaction with CO2 molecules. Aromatic amino acids (Phe, Tyr, and Trp) can form π–π stacking interactions with CO2 molecules that are comparable with common hydrogen bonds (ཞ20 kJ/mol). The best binding sites for CO2 molecules are the three alkaline amino acids (cations Arg+, Lys+, and His+). The acidic amino acids (anions Asp− and Glu−) could maintain a suitable pH condition that is necessary for CO2 releasing from proteins. Histidine may deliver CO2 molecules in proteins through the changes form His+ to His. The CO2 molecules also can form hydrophobic interaction with hydrophobic amino acids, and to be stored in hydrophobic pocket in proteincs. Two peptide absorbents are designed and synthesized, and measured by NMR technique. The study results may help to develop more powerful protain and peptide absorbents for CO2 capture and storage.
Primary myxoid pleomorphic liposarcoma in the anterior mediastinum a case report
Authors: Wen Rui , Tang Jinyan , Hu Xianwen , Shen Xiaotong , Li Chuan , Wang Pan 
Published: September 2023

Abstract
Liposarcoma is a relatively rare malignant tumour of the adipose tissue, can happen in any place, including myxoid pleomorphic liposarcoma (MPL) is a special subtype of liposarcoma, common in children and infants and young children, very low incidence of mediastinum. The literature on mediastinal myxoid pleomorphic liposarcoma published at home and abroad are only case reports, so the clinical symptoms and imaging features are lack of specificity, and the diagnosis is challenging. Finally, postoperative pathology is still needed to confirm the diagnosis. In order to further improve the understanding of myxoid pleomorphic liposarcoma, we report a case of primary mediastinal myxoid pleomorphic liposarcoma with chest pain as the main symptom. The patient was discharged after complete surgical resection. This paper aimed to the disease of clinical, radiological features, pathology, diagnosis and treatment were analyzed and summarized.
Prediction of Water Distribution Uniformity of Sprinkler Irrigation System Based on Machine Learning Algorithms
Authors: Elhussiny Khadiga T. , Hassan Ahmed M. , Habssa Ahmed Abu , Mokhtar Ali 
Published: September 2023

Abstract
The coefficients of uniformity (Christiansen's uniformity coefficient CU and distribution uniformity DU), an important parameter for designing irrigation systems, and is an accurate measure for water lose. That research include, three sprinklers were used to measure the CU and DU results based on operating pressure, heights of sprinkler, discharge, nozzle diameter, wind speed, humidity, highest temperature and lowest temperature to determine the best parameters for good irrigation design and also to select the best scenario effects on CU and DU values for square and triangular system based on four scenarios were applied to predict the DU based on machine learning algorithms. The main findings are 1) the highest CU was 86.7% in the square system of 2520 sprinkler under 200 kPa, height of 0.5m and the discharge was 0.855 m3/h (Nozzle 2.5mm). Furthermore, it was 87.3% in the triangled system under the same pressure and discharge however on the 1m height. 2) In CU, the highest of R2 is 0.796, 0.825 and 0.929 in RF, XGB and XGB-RF respectively in the first scenario. 3) On contrast, for the DU, the highest value of R2 is 0.7014, 0.4791 and 0.8275 in RF, XGB and XGB-RF respectively in the first scenario.
Transforming growth factor-β1 is associated with inflammatory resolution via promoting M2c macrophage polarization in lung injury mice
Authors: Jing Ren , Xie Xianlong , Liao Xiaoting , He Sheng , Mo Jianlan , Dai Huijun , Hu Zhaokun , Pan Linghui 
Published: September 2023

Abstract
Macrophage polarization is vital for the resolution of inflammation and tissue injury. Here, we investigated the role of transforming growth factor (TGF)-β1 on macrophages polarization. In the mouse model of ventilation-induced lung injury, high tidal volume-induced injury and inflammation were resolved 3 days post-ventilation (PV3d) to PV10d, with increased elastic fiber, proteoglycan, and collagen, as well as higher TGF-β1 level. M1 increased temporarily, whereas M2a and M2c began to increase from PV3d. In vivo upregulating or neutralizing TGF-β1 expression indicated that an appropriate expression of TGF-β1 is required to resolve lung injury and inflammation via regulating macrophage polarization. M1 are the main pro-inflammatory macrophages to induce pulmonary edema and inflammation. Inhibition of M1 to M2a is associated with pulmonary injury and inflammation, but M2b is contributed to the attenuation of serum interleukin (IL)-1β and pulmonary IL-6 levels. M2c contributed to the attenuation of serum and pulmonary IL-6 levels as well as serum tumor necrosis factor-α levels. TGF-β1 is a crucial factor to promote M2b and M2c polarization with the reduction of pulmonary IL-6 levels. Together, secretion of TGF-β1-mediated macrophage polarization plays a crucial role in the resolution of inflammatory lung injury.
Nurses’ views of patient- and family-centered care and its practices in peri-operative contexts in hospitals in Northern Ghana
Authors: Adugbire Bernard Atinyagrika , Jordan Portia Janine , Young Cornelle 
Published: September 2023

Abstract
Background
The purpose of the study was to explore the views of nurses on the concept of patient- and family-centered care (PFCC) and its practices in a peri-operative context in Northern Ghanaian hospitals
Methods
The study, using a qualitative explorative, descriptive and contextual research design was conducted at six selected hospitals located in the Northern part of Ghana. Purposive sampling was used to recruit participants for individual and focus group interviews. Data were generated between March and May 2022. Data saturation was reached with 27 participants comprising 15 individuals and 12 members from two focus group interviews. All interviews were digitally recorded and transcribed verbatim and the data analysed using thematic analysis.
Results
Two themes emerged from the study, namely nurses’ understanding of the concept of PFCC and how they see the benefits of PFCC in a peri-operative context. Subthemes of the nurses’ understanding of PFCC included their perceptions of PFCC, the need for collaboration, information sharing and communication and the importance of PFCC practices in a peri-operative context. Subthemes for benefits of PFCC in the peri-operative context included nurse-related benefits, patient- and family-related benefits and healthcare system-related benefits.
Conclusions
The study revealed that the concept PFCC is unfamiliar to the Ghanaian peri-operative context despite the positive perceptions exhibited by the nurses in terms of their understanding of PFCC and its related benefits to nurses, patients and families as well as the healthcare system.
Physical Properties of WR 90 (HD 156385) Based on Spectroscopic Study
Authors: Budi Bakuh Danang Setyo , Malasan Hakim Luthfi 
Published: September 2023

Abstract
The emission line on the spectrum of the Wolf-Rayet star shows an exceptional abundance of the elements helium, nitrogen, carbon, and oxygen w hen compared to the spectrum of other stars. In this work, observation of WR 90 (HD 156385) was conducted using a low-resolution spectrograph at Bosscha Observatory. Determination of stellar parameters has been implemented using two different methods: The first method derives estimation of photosphere temperature and radius using the Black Body continuum model approximation, second method derives estimation of stellar temperature, radius, and luminosity using PoWR (The Postdam Wolf-Rayet) spectrum grid model. Furthermore, with the addition of the asymptotic terminal velocity derived from the Gaussian decomposition of the optical spectrum emission line and the P-Cygni profile analysis of the high-resolution IUE UV spectrum, the mass loss rate of the Wolf-Rayet star has been determined. The data used in both methods are low- and highresolution ultraviolet spectra retrieved from the IUE (International Ultraviolet Explorer) archival and optical spectrum of our observations. The obtained stellar parameters appear to have small differences when compared with previous work.
Multivariate analysis of prognostic factors in patients with lip squamous cell carcinoma after surgery
Authors: Cheng Hao , Xu Jin-Hong , He Jia-Qi , Yang Xi-Yang , Shen Xu-Ning , Xu Xue-Lian 
Published: September 2023

Abstract
Background: Lip squamous cell carcinoma (LSCC) was one of the most common cancer types of head and neck tumors. This study aimed to reveal clinical factors affecting the prognosis of postoperative LSCC patients.
Methods: A total of 147 LSCC patients between June 2012 and June 2018 were collected and analyzed. There were 23 clinicopathological factors included in our study: age at diagnosis, gender, tumor location, pathological differentiation, American Joint Committee on Cancer (AJCC) stage, adjuvant chemotherapy, adjuvant radiotherapy, surgical margin, perineural invasion, extranodal extension (ENE), vascular invasion (VI), multiple primary tumors, age-adjusted Charlson comorbidity index (ACCI); body mass index (BMI), Glasgow prognostic Score (GPS), interquartile range (IQR), neutrophil-to-lymphocyte ratio (NLR), platelet-to-lymphocyte ratio (PLR), prognostic nutrition index (PNI), eastern cooperative oncology group (ECOG) performance status (PS) score, systemic immune-inflammation index(SII), overall survival (OS), and progression-free survival (PFS). The univariate and multivariate Cox regression analyses were applied to reveal the independent prognostic factors in postoperative LSCC patients on PFS and OS. The benefit of adjuvant radiotherapy in various subgroups was also displayed by Kaplan-Meier curves.
Results: In postoperative LSCC patients, the 1-, 3- and 5-year PFS were 88.4%, 70.1%, and 57.8%, and the 1-, 3- and 5-year OS were 94.6%, 76.9%, and 69.4%, respectively. The analysis results indicated that age ≥ 70 years, later stage, surgical margin <5mm, higher PNI, and ACCI ≥5 indicate poorer PFS in postoperative LSCC patients (All P<0.05). Besides, postoperative LSCC patients with age ≥ 70 years, later stage, surgical margin < 5mm, higher GPS score, higher SII, and ACCI ≥5 tend to have a poorer OS (All P<0.05). Additionally, postoperative LSCC patients with ACCI < 5 and AJCC III-IV stage were more likely to benefit from adjuvant radiotherapy, but not for the other subgroups.
Conclusion: Our results showed that clinicopathological factors like age ≥ 70 years, close surgical margin, later stage, surgical margin < 5mm, higher PNI, higher GPS score, higher SII, and ACCI ≥5 had a great negative effect on the prognosis of postoperative LSCC patients. Postoperative LSCC patients with stage III-IV and ACCI < 5 can benefit from adjuvant radiotherapy.
The inherent violence of anti-Black racism and its effects on HIV care for Black sexually minoritized men
Authors: Quinn Katherine G , Walsh Jennifer L , DiFranceisco Wayne , Edwards Travonne , Takahashi Lois , Johnson Anthony , Dakin Andrea , Bouacha Nora , Voisin Dexter R 
Published: September 2023

Abstract
The goal of this study was to examine the effects of racial discrimination, depression, and Black LGBTQ community support on HIV care outcomes among a sample of Black sexually minoritized men living with HIV. We conducted a cross-sectional survey with 107 Black sexually minoritized men living with HIV in Chicago. A path model was used to test associations between racial discrimination, Black LGBTQ community support, depressive symptoms, and missed antiretroviral medication doses and HIV care appointments. Results of the path model showed that men who had experienced more racism had more depressive symptoms and subsequently, missed more doses of HIV antiretroviral medication and had missed more HIV care appointments. Greater Black LGBTQ community support was associated with fewer missed HIV care appointments in the past year. This research shows that anti-Black racism may be a pervasive and harmful determinant of HIV inequities and a critical driver of racial disparities in ART adherence and HIV care engagement experienced by Black SMM. Black LGBTQ community support may buffer against the effects of racial discrimination on HIV care outcomes by providing safe, inclusive, supportive spaces for Black SMM.
Clinical and Economic Outcomes of a Pharmacogenomics-enriched Comprehensive Medication Management Program in a Self-insured Employee Population
Authors: Fragala Maren , Keogh Murray , Goldberg Steven , Lorenz Raymond , Shaman Jeffrey 
Published: September 2023

Abstract

Clinical and economic outcomes from a pharmacogenomics-enriched comprehensive medication management program were evaluated over 26-months in a self-insured employee population (n=452 participants; n=1,500 controls) using propensity matched pre-post design with adjusted negative binomial and linear regression models. After adjusting for baseline covariates, program participation was associated with 39% fewer inpatient (p=0.05) and 39% fewer emergency department (p=0.002) visits, and with 21% more outpatient visits (p<0.001) in the follow-up period compared to the control group. Results show pharmacogenomics-enriched comprehensive medication management can favorably impact healthcare utilization in a self-insured employer population by reducing emergency department and inpatient visits and can offer potential for cost savings. Self-insured employers may consider implementing pharmacogenomics-enriched comprehensive medication management to improve the healthcare of their employees.
Bright quantum photon sources from a topological Floquet resonance
Authors: Barzanjeh Shabir , Afzal Shirin , Zimmerling Tyler , Rizvandi Mahdi , Taghavi Majid , Hrushevskyi Taras , Kaur Manpreet , Van Vien 
Published: September 2023

Abstract
Entanglement, a fundamental concept in quantum mechanics, plays a crucial role as a valuable resource in quantum technologies. The practical implementation of entangled photon sources encounters obstacles arising from imperfections and defects inherent in physical systems and microchips, resulting in a loss or degradation of entanglement. The topological photonic insulators, however, have emerged as promising candidates, demonstrating an exceptional capability to resist defect-induced scattering, thus enabling the development of robust entangled sources.
Despite their inherent advantages, building bright and programmable topologically protected entangled sources remains challenging due to intricate device designs and weak material nonlinearity. Here we present an advancement in entanglement generation achieved through a non-magnetic and tunable resonance-based anomalous Floquet insulator, utilizing an optical spontaneous four-wave mixing process. Our experiment demonstrates a substantial enhancement in entangled photon pair generation compared to devices reliant solely on topological edge states and outperforming trivial photonic devices in spectral resilience. This work marks a step forward in the pursuit of defect-robust and bright entangled sources that can open avenues for the exploration of cascaded quantum devices and the engineering of quantum states. Our result could lead to the development of resilient quantum sources with potential applications in quantum technologies.
Linear responses of soil microbiomes, metagenomic and metabolomic functioning across ecosystems along water gradients in the Altai region, northwestern China
Authors: Fu Qi , Qiu Yingbo , Zhao Jiayi , Li Jiaxin , Xie Siqi , Liao Qiuchang , Fu Xianheng , Huang Yu , Yao Zhiyuan , Dai Zhongmin , Qiu Yunpeng , Li Furong , Chen Huaihai 
Published: September 2023

Abstract
Background
To investigate microbial communities and their contributions to carbon and nutrient cycling along water gradients can enhance our comprehension of climate change impacts on ecosystem services.
Results
We conducted an assessment of microbial communities, metagenomic functions, and metabolomic profiles within four ecosystems, i.e., desert grassland (DG), shrub-steppe (SS), forest (FO) and marsh (MA) in the Altai region of Xinjiang, China. Soil total carbon (TC), total nitrogen, NH4+, and NO3- increased linearly, but pH decreased with soil water gradients. Microbial abundances and richness also increased with soil moisture except the abundances of fungi and protists being lowest in MA. Within prokaryotes, the relative abundances of Proteobacteria and Acidobacteria increased, whereas those of Actinobacteria and Thaumarchaeota decreased along water gradients. In fungi and protists, Basidiomycota and Mortierellomycota, Evosea and Endomyxa became dominant in FO and MA, respectively, but the relative abundance of Cercozoa decreased along soil moisture gradients. The β-diversity of microbiomes, metagenomic and metabolomic functioning were linearly distributed along soil moisture gradients, significantly associated with soil factors of TC, NH4+, and pH. For soil metagenomic functions, the metabolic genes related to Carbohydrate (CO2 fixation, Di- and oligosaccharides, Fermentation, and One-carbon metabolism), Iron (Iron acquisition in Vibrio and Campylobacter iron metabolism) decreased with soil moisture, while genes related to the metabolisms of Nitrogen (Ammonia assimilation, Denitrification, Nitrogen fixation, and Nitrosative stress) and Potassium (Potassium homeostasis) increased linearly along water gradients. Additionally, MA harbored the most abundant metabolomics dominated by lipids and lipid-like molecules (Erucic acid, Hypogeic acid, and Kojibiose, etc.), and organic oxygen compounds (Maltotetraose, Quinone, Sucrose, and Trehalose, etc.), except certain metabolites showing decline trends along water gradients, such as N'-Hydroxymethylnorcotinine and 5-Hydroxyenterolactone.
Conclusions
Our study suggests that future ecosystem succession facilitated by changes in rainfall patterns will significantly alter soil microbial taxa, functional potential and metabolite fractions.
Genome-wide Identification of the CIPK Gene Family in Jasmine and Expression Analysis Under Salt Stress
Authors: Chen Kai , Cao Tingting , Yin Lili , Huang Xin , Zhang Xuyao , Wu Ruigang 
Published: September 2023

Abstract
Background
Various CIPK genes are involved in abiotic stress responses in plants. Despite the economic importance of jasmine (Jasminum sambac), and the availability of genome data, there are few reports analyzing the CIPK gene family. The aim of this study was to perform a phylogenetic analysis and characterization of the CIPK genes in jasmine.
Results
In the present study, A total of 17 CIPKs were identified, which were unevenly distributed on chromosomes. The JsCIPK protein sequences contained 311–781 amino acids, with a molecular weight of 35.05–87.58 kDa. Phylogenetic analysis revealed that the 17 JsCIPKs could be divided into five classical branches. JsCIPK genes with higher homology showed greater similarity between conserved protein motifs. Collinearity analysis demonstrated that 13 gene pairs in Arabidopsis were collinear with the jasmine sequences. Various hormone-related response- and stress-induced elements were observed in the promoter region of JsCIPK genes. Furthermore, the expression of JsCIPK genes varied in different organs. Finally, the expression analyses of eight JsCIPKs under salt stress were performed.
Conclusions
A systematic analysis of the CIPK gene family in Jasmine and expression analysis under salt stress was provided. These findings lay a foundation for future functional studies of JsCIPKs in jasmine related to growth and development and responses to abiotic stress.