bluuebunny/crossref_metadata_2025_split huggingface dataset:
Systematic analysis and prediction for disease burden of ovarian cancer attributable to hyperglycemia: a comparative study between China and the world from 1990 to 2019
Authors: Wu Peihong , Jiang Qingtao , Han Lei , Liu Xin 
Published: April 2023

BackgroundOvarian cancer is one of the most common female malignancies worldwide, and metabolic factors, such as hyperglycemia, are becoming potential risk factors. This study aimed to analyze the disease burden and its changing trend of ovarian cancer attributable to hyperglycemia in the Chinese population from 1990 to 2019.MethodsUsing the data released by the Global Burden of Disease study 2019 (GBD 2019), we analyze the disease burden of ovarian cancer attributable to hyperglycemia in Chinese from 1990 to 2019 via morbidity, death, disability-adjusted life years (DALY); compare it with the global population; and predict the incidence and death trend in Chinese women for the next 10 years (2020–2029).ResultsThe incidence, death cases, and DALY numbers of ovarian cancer attributable to hyperglycemia in Chinese in 2019 were 2,751, 1,758, and 44,615 person-years, respectively, with an increase of 352.5%, 356.6%, and 329.0% compared with 1990, and the growth rate was higher than the global level. The age-standardized incidence rate (ASIR), age-standardized mortality rate (ASMR), and age-standardized DALY rate (ASDR) in 2019 were 0.270/100,000, 0.164/100,000, and 4.103/100,000, respectively. Moreover, the average annual percent changes (AAPCs) were 2.3%, 2.0%, and 2.0%, respectively, all higher than the global average. The disease burden of ovarian cancer attributable to hyperglycemia increased with age, reaching a peak in the 45–75 age group. The prediction of the neural network model showed that the incidence and death of the disease would remain high and rise in the next 10 years.ConclusionThe disease burden caused by ovarian cancer attributable to hyperglycemia in Chinese accounts for a large proportion globally, and the ASIR, ASMR, and ASDR are increasing year by year. We should continue to pay attention to the role of metabolic factors, such as hyperglycemia, in the occurrence and development of ovarian cancer, perform a good job in tertiary prevention, and strive to reduce health losses.
New insights into the genome and transmission of the microsporidian pathogen Nosema muscidifuracis
Authors: Xiong Xiao , Geden Christopher J. , Bergstralh Dan T. , White Roxie L. , Werren John H. , Wang Xu 
Published: April 2023

IntroductionNosema is a diverse genus of unicellular microsporidian parasites of insects and other arthropods. Nosema muscidifuracis infects parasitoid wasp species of Muscidifurax zaraptor and M. raptor (Hymenoptera: Pteromalidae), causing ~50% reduction in longevity and ~90% reduction in fecundity.Methods and ResultsHere, we report the first assembly of the N. muscidifuracis genome (14,397,169 bp in 28 contigs) of high continuity (contig N50 544.3 Kb) and completeness (BUSCO score 97.0%). A total of 2,782 protein-coding genes were annotated, with 66.2% of the genes having two copies and 24.0% of genes having three copies. These duplicated genes are highly similar, with a sequence identity of 99.3%. The complex pattern suggests extensive gene duplications and rearrangements across the genome. We annotated 57 rDNA loci, which are highly GC-rich (37%) in a GC-poor genome (25% genome average). Nosema-specific qPCR primer sets were designed based on 18S rDNA annotation as a diagnostic tool to determine its titer in host samples. We discovered high Nosema titers in Nosema-cured M. raptor and M. zaraptor using heat treatment in 2017 and 2019, suggesting that the remedy did not completely eliminate the Nosema infection. Cytogenetic analyses revealed heavy infections of N. muscidifuracis within the ovaries of M. raptor and M. zaraptor, consistent with the titer determined by qPCR and suggesting a heritable component of infection and per ovum vertical transmission.DiscussionThe parasitoids-Nosema system is laboratory tractable and, therefore, can serve as a model to inform future genome manipulations of Nosema-host system for investigations of Nosemosis.
GDNet-EEG: An attention-aware deep neural network based on group depth-wise convolution for SSVEP stimulation frequency recognition
Authors: Wan Zhijiang , Cheng Wangxinjun , Li Manyu , Zhu Renping , Duan Wenfeng 
Published: April 2023

BackgroundSteady state visually evoked potentials (SSVEPs) based early glaucoma diagnosis requires effective data processing (e.g., deep learning) to provide accurate stimulation frequency recognition. Thus, we propose a group depth-wise convolutional neural network (GDNet-EEG), a novel electroencephalography (EEG)-oriented deep learning model tailored to learn regional characteristics and network characteristics of EEG-based brain activity to perform SSVEPs-based stimulation frequency recognition.MethodGroup depth-wise convolution is proposed to extract temporal and spectral features from the EEG signal of each brain region and represent regional characteristics as diverse as possible. Furthermore, EEG attention consisting of EEG channel-wise attention and specialized network-wise attention is designed to identify essential brain regions and form significant feature maps as specialized brain functional networks. Two publicly SSVEPs datasets (large-scale benchmark and BETA dataset) and their combined dataset are utilized to validate the classification performance of our model.ResultsBased on the input sample with a signal length of 1 s, the GDNet-EEG model achieves the average classification accuracies of 84.11, 85.93, and 93.35% on the benchmark, BETA, and combination datasets, respectively. Compared with the average classification accuracies achieved by comparison baselines, the average classification accuracies of the GDNet-EEG trained on a combination dataset increased from 1.96 to 18.2%.ConclusionOur approach can be potentially suitable for providing accurate SSVEP stimulation frequency recognition and being used in early glaucoma diagnosis.
Hulless Black Barley as a Carrier of Probiotics and a Supplement Rich in Phenolics Targeting Against H2O2-Induced Oxidative Injuries in Human Hepatocarcinoma Cells
Authors: Wu Han , Liu Hao-Nan , Liu Chun-Quan , Zhou Jian-Zhong , Liu Xiao-Li , Zhang Hong-Zhi 
Published: April 2023

Graphical AbstractTransformation of barley phenolics by fermentation improves their antioxidant capacities in chemical and cell levels.
Interactions between curcumin and human salt-induced kinase 3 elucidated from computational tools and experimental methods
Authors: Shi Mingsong , Zhou Yan , Wei Haoche , Zhang Xinyu , Du Meng , Zhou Yanting , Yin Yuan , Li Xinghui , Tang Xinyi , Sun Liang , Xu Dingguo , Li Xiaoan 
Published: April 2023

Natural products are widely used for treating mitochondrial dysfunction-related diseases and cancers. Curcumin, a well-known natural product, can be potentially used to treat cancer. Human salt-induced kinase 3 (SIK3) is one of the target proteins for curcumin. However, the interactions between curcumin and human SIK3 have not yet been investigated in detail. In this study, we studied the binding models for the interactions between curcumin and human SIK3 using computational tools such as homology modeling, molecular docking, molecular dynamics simulations, and binding free energy calculations. The open activity loop conformation of SIK3 with the ketoenol form of curcumin was the optimal binding model. The I72, V80, A93, Y144, A145, and L195 residues played a key role for curcumin binding with human SIK3. The interactions between curcumin and human SIK3 were also investigated using the kinase assay. Moreover, curcumin exhibited an IC50 (half-maximal inhibitory concentration) value of 131 nM, and it showed significant antiproliferative activities of 9.62 ± 0.33 µM and 72.37 ± 0.37 µM against the MCF-7 and MDA-MB-23 cell lines, respectively. This study provides detailed information on the binding of curcumin with human SIK3 and may facilitate the design of novel salt-inducible kinases inhibitors.
A modified formula using energy system contributions to calculate pure maximal rate of lactate accumulation during a maximal sprint cycling test
Authors: Yang Woo-Hwi , Park So-Young , Kim Taenam , Jeon Hyung-Jin , Heine Oliver , Gehlert Sebastian 
Published: April 2023

Purpose: This study aimed at comparing previous calculating formulas of maximal lactate accumulation rate (νLa.max) and a modified formula of pure νLa.max (PνLa.max) during a 15-s all-out sprint cycling test (ASCT) to analyze their relationships.Methods: Thirty male national-level track cyclists participated in this study (n = 30) and performed a 15-s ASCT. The anaerobic power output (Wpeak and Wmean), oxygen uptake, and blood lactate concentrations (La−) were measured. These parameters were used for different calculations of νLa.max and three energy contributions (phosphagen, WPCr; glycolytic, WGly; and oxidative, WOxi). The PνLa.max calculation considered delta La−, time until Wpeak (tPCr−peak), and the time contributed by the oxidative system (tOxi). Other νLa.max levels without tOxi were calculated using decreasing time by 3.5% from Wpeak (tPCr −3.5%) and tPCr−peak.Results: The absolute and relative WPCr were higher than WGly and WOxi (p &lt; 0.0001, respectively), and the absolute and relative WGly were significantly higher than WOxi (p &lt; 0.0001, respectively); νLa.max (tPCr −3.5%) was significantly higher than PνLa.max and νLa.max (tPCr−peak), while νLa.max (tPCr−peak) was lower than PνLa.max (p &lt; 0.0001, respectively). PνLa.max and νLa.max (tPCr−peak) were highly correlated (r = 0.99; R2 = 0.98). This correlation was higher than the relationship between PνLa.max and νLa.max (tPCr −3.5%) (r = 0.87; R2 = 0.77). νLa.max (tPCr−peak), PνLa.max, and νLa.max (tPCr −3.5%) were found to correlate with absolute Wmean and WGly.Conclusion: PνLa.max as a modified calculation of νLa.max provides more detailed insights into the inter-individual differences in energy and glycolytic metabolism than νLa.max (tPCr−peak) and νLa.max (tPCr −3.5%). Because WOxi and WPCr can differ remarkably between athletes, implementing their values in PνLa.max can establish more optimized individual profiling for elite track cyclists.
Morally injurious events among aid workers: examining the indirect effect of negative cognitions and self-care in associations with mental health indicators
Authors: Dewar Michelle , Paradis Alison , Brillon Pascale 
Published: April 2023

IntroductionPotentially morally injurious events (PMIE) are events that violate one’s deeply held moral values or beliefs, and that have the potential to create significant inner conflict and psychological distress. PMIE have been recognized as an important psychological risk factor in many high-risk occupational groups. However, no study to date has investigated how PMIE relate to the mental health of aid workers. Furthermore, little is known about the mechanisms by which PMIE might be associated with mental health indicators.MethodsParticipants were 243 aid workers (72% female; Mage = 39.31) who had completed at least one aid assignment (M = 8.17). They completed an online questionnaire about their PMIE, trauma history, and mental health. A structural equation model was constructed to examine the roles of negative cognitions and subsequent self-care behaviors in the relationship between PMIE and PTSD symptoms, depression symptoms, and posttraumatic growth, above and beyond the contribution of potentially traumatic events.ResultsWithin the model, the indirect effect through negative cognitions fully accounted for the associations between PMIE and symptoms of PTSD and depression. For the association between PMIE and posttraumatic growth, two indirect effects emerged: the first through negative cognitions and subsequent self-care and, the second, through self-care alone.DiscussionThis study highlighted PMIE as a novel psychological risk factor for aid workers and pointed to two possible mechanisms by which these events may lead to PTSD, depression, and posttraumatic growth. This study adds to the current understanding of how high-risk occupational groups adapt psychologically to PMIE.
Evaluation of a virtual, simulated international public health peer-to-peer exchange learning experience
Authors: Ahmad Danish , McFarlane Rosemary A. , Smith Jennifer , Saxena Deepak , Somerset Shawn , Mavalankar Dileep 
Published: April 2023

IntroductionPublic Health’s (PH) global rise is accompanied by an increasing focus on training the new generation of PH graduates in interdisciplinary skills for multisectoral and cross-cultural engagement to develop an understanding of commonalities in health system issues and challenges in multi-cultural settings. Online teaching modalities provide an opportunity to enhance global health skill development through virtual engagement and peer exchange. However, current teaching pedagogy is limited in providing innovative modes of learning global health issues outside of traditional classroom settings with limited modalities of evidence-informed implementation models.MethodsThis study designed, implemented, and evaluated a novel global health online synchronous module as proof of concept that incorporated elements of virtual Practice-based learning (PBL) using a case study approach offered to currently enrolled public health students at the University of Canberra (UC) and a partnering public health university from India, the Indian Institute of Public Health Gandhinagar (IIPH-G). Using constructive learning theory and the Social Determinants of Health framework, four online sessions were designed and implemented in August–September 2022. Formal process and outcome evaluation using a quantitative adapted survey of the validated International Student Experience survey (IES) at session end and findings provided.ResultsOver 100 participating public health students from Australia and India provided narrative feedback and quantitative responses from the adapted IES instrument across four key dimensions, namely “motivation,” “personal development,” intellectual development, and “international perspectives” reporting an overall high mean impact of 4.29 (out of 5) across all four themes seen together. In essence, the sessions supported students to explore global health issues from a different cultural perspective while developing intercultural communication skills and enhancing their global exposure in real-time.DiscussionsThis innovation, implemented as a proof of concept, provided evidence, and demonstrated the implementation feasibility of a flexible virtual integrated practice-based module that can supplement classroom teaching. It provides participating students with the opportunity to develop intercultural understanding and communication competence as well as support global mindedness by engaging with international peers around focused global health case studies.
Establishment of an optimized guinea pig model of cisplatin-induced ototoxicity
Authors: Ahmadi Navid , Saidov Nodir , Gausterer Julia Clara , Kramer Anne-Margarethe , Honeder Clemens , Arnoldner Christoph 
Published: April 2023

BackgroundCisplatin is among the most effective antineoplastic agents and has revolutionized the treatment of many cancer diseases. However, one of its serious side effects is a progressive and irreversible hearing loss, occurring in both adults and children. For the development of otoprotective therapies that prevent this side effect, cisplatin-induced hearing loss animal models are indispensable. Due to the high toxicity of cisplatin, the establishment of such animal models is a difficult and time-consuming task. Here we introduce the detailed protocol of a sophisticated guinea pig model with a sufficient and permanent hearing loss induced by cisplatin. This manuscript is intended to provide guidance in the development of future cisplatin guinea pig models which may reduce the mortality rate of the animals and help to gain more reproducible results.MethodsPigmented and unpigmented guineapigs were treated with an intravenous single application of 8 mg/kg cisplatin under general anesthesia. An extensive and long-term intensive care protocol consisting of scheduled application of fluids, antiemetics, analgesics, glucose and supportive feeding among others, was used to ensure wellbeing of the animals. Hearing tests were performed prior to and 5 days after cisplatin application. Animals were then euthanized.ResultsThe ABR audiometry 5 days after cisplatin application revealed a hearing threshold ranging from 70 dB to 90 dB in the frequencies from 1 kHz to 32 kHz respectively.All animals presented a good health condition despite the treatment with cisplatin.DiscussionThe introduced care protocol in this manuscript is intended to serve as a guidance for the establishment of a stable guinea pig model for short- and long-term investigation regarding the inner ear and its protection in the frame work of cisplatin-induced damage.
Biological Activity and Component Analyses of Chamaecyparis obtusa Leaf Extract: Evaluation of Antiwrinkle and Cell Protection Effects in UVA-Irradiated Cells
Authors: Jang Young-Ah , Kim Se-Gie , Kim Hye-Kyung , Lee Jin-Tae 
Published: April 2023

Background and Objectives: Chamaecyparis obtusa (C. obtuse) extract has been used as a folk medicinal remedy in East Asian countries to alleviate inflammation and prevent allergies. Active oxygen causes skin aging and leads to skin cell and tissue damage. Extensive research has been conducted to control active oxygen generation to prevent skin aging. We evaluated the antioxidant activity and antiwrinkle effect of C. obtusa extract to determine its potential as a cosmetic material. Materials and Methods: The antioxidant activity of a 70% ethanol extract of C. obtusa (COE 70) and a water extract of C. obtusa (COW) was determined using 2,2-diphenyl-1-picrylhydrazy (DPPH) scavenging, 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS+) scavenging, superoxide dismutase-like activity, xanthine oxidase inhibition, and ferric-reducing antioxidant power assays. The effective concentration of the extracts was determined using the methyl thiazolyl tetrazolium assay to evaluate their toxicity. The effects of COE 70 on the production of matrix metalloproteinases (MMPs) and procollagen, and expression of activated cytokines, interleukin 6 (IL-6) and tumor necrosis factor α (TNF-α), in UVA-irradiated fibroblasts were determined using quantitative real-time PCR. Additionally, quercitrin, amentoflavone, hinokiflavone, and myricetin concentrations in COE 70 were determined using high-pressure high-performance liquid chromatography. Results: COE 70 had higher polyphenol and flavonoid concentrations than COW and exhibited an excellent antioxidant effect. COE 70 suppressed UVA-induced fibroblast death by 21.3% at 25 µg/mL. It also increased MMP-1, MMP-3, TNF-α, and IL-6 mRNA levels at 5–25 µg/mL compared with those in control UVA-irradiated fibroblasts. Moreover, mRNA levels of collagen type I and superoxide dismutase significantly increased, indicating the antiwrinkle and anti-inflammatory effects of the extract. Among the COE 70 components, quercitrin concentration was the highest; hence, quercitrin could be an active ingredient. Conclusions: COE 70 could be used as a natural antioxidant and antiwrinkle agent.
The Effect of Glycine Betaine on Nitrogen and Polyamine Metabolisms, Expression of Glycoside-Related Biosynthetic Enzymes, and K/Na Balance of Stevia under Salt Stress
Authors: Ghorbani Abazar , Ghasemi-Omran Vali Ollah , Chen Moxian 
Published: April 2023

The beneficial role of glycine betaine (GB) in the adaptation of plants to abiotic stresses is well known; therefore, the study of physiological and molecular responses induced by exogenous GB under NaCl stress can provide a suitable reference for the application of this compound to enhance the adaptation of plants to salinity. The present study was conducted under in vitro conditions to evaluate the effect of GB (25 and 50 mM) on the growth, physiological, and molecular traits of Stevia rebaudiana during NaCl toxicity (50 mM). The results showed that applying NaCl treatment increased Na accumulation, induced oxidative stress, and disrupted N metabolism and K/Na homeostasis, which, as a result, decreased the stevia plant’s growth and biomass. However, application of GB improved the adaptation of NaCl-stressed plants by improving N metabolism and modulating the metabolism of polyamines. By increasing the activity of antioxidant enzymes, GB diminished oxidative stress, protected the plasma membrane, and restored photosynthetic pigments under NaCl toxicity. By reducing Na accumulation and increasing K accumulation, GB maintained the K/Na balance and reduced the effects of toxicity caused by the high Na concentration in stevia leaves. GB increased the leaf accumulation of rebaudioside A in NaCl-stressed plants by modulating the expression of genes (KAH, UGT74G1, UGT76G1, and UGT85C2) involved in the sugar compounds of the stevia plants. Our results provide a broad understanding of GB-induced responses in NaCl-stressed plants, which can help increase our knowledge of the role of GB in the defense mechanisms of plants under abiotic stresses.
Aplicação da logística reversa na construção civil como mecanismo ambiental sustentável em políticas públicas / Application of reverse logistics in civil construction as a sustainable environmental mechanism in public policies
Authors: Fonseca Maria Julia M. , Maintinguer Sandra I. , Maintinguer Sandra I. 
Published: April 2023

A construção civil consome até 75% dos recursos naturais extraídos e é um dos setores que mais geram resíduos, causando expressivos impactos ambientais. As políticas públicas para o desenvolvimento sustentável da construção civil prescrevem o comprometimento com o uso dos recursos naturais e a disposição correta de seus resíduos. A logística reversa é um instrumento de política pública na gestão dos resíduos sólidos, disposta na Lei 12.305/2010 (Política Pública de Resíduos Sólidos – PNRS), que preconiza a reinserção de materiais ao mercado por meio da reciclagem ou reuso, atuando no processo de planejamento e controle, tornando-se um importante mecanismo de desenvolvimento social, econômico e sustentável. A Política Nacional de Resíduos Sólidos em seu artigo 33 traz a responsabilidade integrada em implementar a logística reversa para todos os agentes envolvidos no ciclo de vida dos produtos. Assim, os gestores do setor da construção civil seriam obrigados a dar uma destinação adequada aos resíduos sólidos da construção civil. No Brasil, embora a logística reversa ainda seja um assunto pouco abordado, a criação de programas como a ISO 14000 pode alavancar a construção civil, alcançando objetivos mais sustentáveis de redução de resíduos gerados, minimizando seu impacto ambiental. Dessa forma, o objetivo desse estudo foi verificar o papel da logística reversa como mecanismo ambiental sustentável na construção de políticas públicas atuantes na gestão de resíduos gerados na construção civil, através da leitura exploratória de artigos concernentes ao assunto e das imposições e inovações trazidas pela Legislação Ambiental.