bluuebunny/crossref_metadata_2025_split huggingface dataset:
Salivary macrophage activation‐related chemokines and mitogen‐activated kinase kinase‐degrading proteolytic activity in type 1 diabetes mellitus
Authors: Yilmaz Neslihan , Polat Recep , Gürsoy Mervi , Kaman Wendy , Gül Aydin Elif , Fteita Dareen , Yilmaz Dogukan , Bikker Floris , Gürsoy Ulvi Kahraman 
Published: January 2023

AbstractBackgroundThis cross‐sectional study aimed to evaluate salivary concentrations of macrophage activation‐related chemokines and mitogen‐activated kinase kinase (MAPKK)‐degrading proteolytic activity in children and adolescents with and without type 1 diabetes mellitus (T1DM).MethodsA total of 122 children and adolescents (65 T1DM patients, 50.8% female, mean age:10.9 years; 57 systemically healthy controls, 36.8% female, mean age: 9.5 years) were included in the study. Salivary concentrations of interferon gamma inducible protein‐10 (IP‐10), monocyte chemoattractant protein (MCP)‐1, MCP‐2, MCP‐3, MCP‐4, macrophage‐derived chemokine (MDC), macrophage migration inhibitory factor (MIF), monokine induced by interferon gamma (MIG), and macrophage inflammatory protein‐1 alpha (MIP‐1α) were quantified using a bead‐based technique. MAPKK‐degrading proteolytic activity was detected using fluorescent peptide substrates.ResultsThe T1DM group had higher plaque index (PI%, p = 0.032) and bleeding on probing (BOP%, p = 0.045) scores, and lower decayed, missing, filled teeth (dmft/DMFT, p = 0.002) index scores compared to the healthy controls. Compared to the controls, salivary MCP‐1 (p = 0.007), MCP‐3 (p < 0.001), MIG (p = 0.007), and MIP‐1α (p = 0.033) concentrations were elevated whereas MCP‐4 concentrations decreased (p < 0.001) in the T1DM group. After adjusting for age, PI%, BOP%, and dmft/DMFT scores, significant differences in salivary concentrations of MIG (p = 0.033) and MIP‐1α (p = 0.017) were observed between the groups. Moreover, protease activities directed to the cleavage sites of MEK23‐18 (p = 0.001), MKK6b7‐22 (p = 0.007), MKK451‐66 (p = 0.005), MKK7b37‐52 (p = 0.034), and MKK7b69‐84 (p = 0.009) were elevated in the T1DM group.ConclusionT1DM disrupts the salivary macrophage activation‐related chemokine profile and dysregulates proteolytic MAPKK cleavage. These findings can be an outcome of the impaired systemic immune response in T1DM.
How can customers cope with cognitive demands of professional services? The role of employee coping support
Authors: Garbas Janina , Blaurock Marah , Büttgen Marion , Ates Zelal 
Published: January 2023

AbstractEven though researchers are increasingly acknowledging the dark side of customer participation (i.e., behavioral customer engagement), particularly in professional services with high cognitive demands that cause customer participation stress (i.e., negative psychological state resulting from the customer's overextension by required customer participation efforts), insights on how firms can effectively mitigate customer participation stress remains limited. Building on transactional stress theory, we investigate whether customers can effectively cope with the expected cognitive demands of professional services. Moreover, by introducing an adapted coping construct (i.e., coping support), we examine whether employees can provide coping support to help decrease customer participation stress. The findings of a time‐lagged field study with customers of a large German bank (N = 117) suggest that customer coping before the encounter cannot mitigate the effect of anticipated cognitive demands on customer participation stress. Instead, the results of both the field study and a follow‐up experimental study (N = 218) show that a certain set of employee coping support during service encounters is crucial. While focusing on action coping support is not ideal in situations with high cognitive demands, firms should advise their professional service employees to offer emotional coping support to attenuate the unfavorable effect of cognitive demands on customer participation stress.
Exploring role strain and experiences of caregivers of children living with disabilities
Authors: Sakwape Keletwaetse , Machailo Rorisang , Koen Magdalene P. 
Published: January 2023

AbstractAimTo explore and describe caregiver role strain in caregivers of children living with disabilities.DesignThis study used qualitative explorative, descriptive and contextual design.MethodsEleven participants were purposively chosen from caregivers of children receiving help from the social and community development office. Data collection used semistructured interviews and data saturation occurred with the 11 participants. The data were analysed using content analysis process. The Consolidated Criteria for Reporting Qualitative Research Guidelines were used for reporting.ResultsFour main themes were identified: caring for a child with disability affects the overall health of the caregiver; caring for children living with disabilities puts financial strain on the caregivers; caring for children living with disabilities has an impact on other roles; and strategies employed to cope with the strain of caring for a child living with disability.
The Reductive Leaching of Chalcopyrite by Chromium(II) Chloride for the Rapid and Complete Extraction of Copper
Authors: Vardner Jonathan T. , Inaba Yuta , Jung Heejung , Farinato Raymond S. , Nagaraj D. R. , Banta Scott , West Alan C. 
Published: January 2023

AbstractA hydrometallurgical process is developed to lower the costs of copper production and thereby sustain the use of copper throughout the global transition to renewable energy technologies. The unique feature of the hydrometallurgical process is the reductive treatment of chalcopyrite, which is in contrast to the oxidative treatment more commonly pursued in the literature. Chalcopyrite reduction by chromium(II) ion is described for the first time and superior kinetics are shown. At high concentrate loadings of 39, 78, and 117 g L−1, chalcopyrite reacted completely within minutes at room temperature and pressure. The XRD, SEM‐EDS, and XPS measurements indicate that chalcopyrite reacts to form copper(I) chloride (CuCl). After the reductive treatment, the mineral products are leached by iron(III) sulfate to demonstrate the complete extraction of copper. The chromium(II) ion may be regenerated by an electrolysis unit inspired by an iron chromium flow battery in a practical industrial process.
Enhancing the mechanical properties of fiber metal laminate by surface treatment
Authors: Zou Hang , Chen Yong , Zhang Xiaodong , Ning Conggang , Pan Ruqing , Yang Youkun , Zhang Junshuang , Du Bing 
Published: January 2023

AbstractFiber‐metal laminates with high toughness and high strength have become an important direction for the development of new materials in the future. During the production and service process of fiber‐metal laminates, brittle fracture and interlaminate damage are easily caused by low load, which leads to the instability and damage to the overall structure. This article mainly studies the effects of surface treatment process, and material combination on the mechanical properties of glass fiber/polypropylene (PP)/stainless steel mesh (SSM) composite laminates. It is shown that the adhesion force of the phosphating SSM sample is 1.73 times that of the etched sample, and the bonding effect with organic substances is relatively better. The laminate with the structure of “unetched SSM + maleic anhydride grafted polypropylene + aminosilane glass fiber fabric” had the best tensile load. The etched and phosphatized SSM enhanced the interface adhesive effect with PP‐g‐MAH, thereby improving the overall structural strength of the laminate.
Novel fluorescence spectroscopy method coupled with N‐PLS‐R and PLS‐DA models for the quantification of cannabinoids and the classification of cannabis cultivars
Authors: Birenboim Matan , Kenigsbuch David , Shimshoni Jakob A. 
Published: January 2023

AbstractIntroductionCannabis sativa L. inflorescences are rich in secondary metabolites, particularly cannabinoids. The most common techniques for elucidating cannabinoid composition are expensive technologies, such as high‐pressure liquid chromatography (HPLC).ObjectivesWe aimed to develop and evaluate the performance of a novel fluorescence spectroscopy‐based method coupled with N‐way partial least squares regression (N‐PLS‐R) and partial least squares discriminant analysis (PLS‐DA) models to replace the expensive chromatographic methods for preharvest cannabinoid quantification.MethodologyFresh medicinal cannabis inflorescences were collected and ethanol extracts were prepared. Their excitation–emission spectra were measured using fluorescence spectroscopy and their cannabinoid contents were determined by HPLC‐PDA. Subsequently, N‐PLS‐R and PLS‐DA models were applied to the excitation–emission matrices (EEMs) for cannabinoid concentration prediction and cultivar classification, respectively.ResultsThe N‐PLS‐R model was based on a set of EEMs (n = 82) and provided good to excellent quantification of (−)‐Δ9‐trans‐tetrahydrocannabinolic acid, cannabidiolic acid, cannabigerolic acid, cannabichromenic acid, and (−)‐Δ9‐trans‐tetrahydrocannabinol (R2CV and R2pred > 0.75; RPD > 2.3 and RPIQ > 3.5; RMSECV/RMSEC ratio < 1.4). The PLS‐DA model enabled a clear distinction between the four major classes studied (sensitivity, specificity, and accuracy of the prediction sets were all ≥0.9).ConclusionsThe fluorescence spectral region (excitation 220–400 nm, emission 280–550 nm) harbors sufficient information for accurate prediction of cannabinoid contents and accurate classification using a relatively small data set.
Electrospun nanoyarns: A comprehensive review of manufacturing methods and applications
Authors: Kamireddi Divya , Street Reva M. , Schauer Caroline L. 
Published: January 2023

AbstractThe global smart textile industry is estimated to reach 5.9 billion by 2026. However current textiles are limited due to the number of materials that can be incorporated into textiles that provide functionality and due to the use of bulky and rigid electronics. Nanofibers provide an avenue to address these limitations. Additionally, by incorporating nanofibers into textiles, functionality, and properties can be controlled from the nanoscale to the macroscale. A new method of incorporating nanofibers into textiles is by transforming nanofibers into nanoyarns. Nanoyarns would expand the applications of smart textiles to include bioactive scaffolds, tissue engineering, sensors, solar cells, and batteries. This review paper provides a comprehensive overview of different manufacturing methods for producing nanoyarns and their applications in tissue engineering and energy.
Topping and grafting affect the alkaloid content and gene expression patterns of tobacco (Nicotiana tabacumL.)
Authors: Zhang Mengyue , Zhao Yuanyuan , Shi Hongzhi 
Published: January 2023

AbstractIn this study, we aimed to determine molecular mechanisms underlying the effects of topping and grafting in tobacco (Nicotiana tabacumL.) by comparing the alkaloid contents and enrichment pathways of differentially expressed genes (DEGs) among plants subjected to different combinations of topping and grafting treatments. Plants of the tobacco variety “Zhongyan 100” and eggplant (Solanum melongenaL.) were grafted in four combinations as scions and rootstocks, respectively. The four treatment groups were tobacco with topping, tobacco without topping, topped tobacco grafted onto an eggplant rootstock, and non‐topped tobacco grafted onto an eggplant rootstock. Tobacco leaves were collected on the day of topping, at 7 days after topping, and after flue curing, the alkaloid contents of the collected leaves were determined. Leaves of plants subjected to the different treatments were collected for RNA sequencing and screened for DEGs, which were subsequently subjected to functional enrichment analyses. Analyses revealed reductions in the leaf alkaloid contents of tobacco subjected to combined topping and eggplant grafting. Gene annotation indicated that topping influences biological processes such as starch metabolism and stress response, whereas grafting affected the biosynthesis and metabolic pathways of secondary metabolites. Downregulated DEGs between non‐topped tobacco and eggplant‐grafted topped tobacco and between topped and non‐topped tobacco are mainly involved in inositol phosphate metabolic and biosynthetic processes. Downregulated DEGs between different grafting methods (eggplant‐grafted non‐topped tobacco vs. non‐topped tobacco and eggplant‐grafted topped tobacco vs. topped tobacco) are mainly involved in sesquiterpene synthase activity and photosynthesis. The findings of this study provide important insights into the molecular mechanisms underlying the effects of topping and grafting on tobacco plants.
Antimicrobial activity of Xylopia pancheri Baill. Leaf extract against susceptible and resistant Staphylococcus aureus
Authors: Werler Nadine , Guentas Linda , Colot Julien , Hnawia Edouard , Matsui Mariko , Nour Mohammed 
Published: January 2023

AbstractAntibacterial activity of Xylopia pancheri Baill. (Annonaceae) leaf extract was investigated against susceptible Staphylococcus aureus, methicillin‐resistant S. aureus (MRSA) and extended‐spectrum‐β‐lactamase (ESBL)‐producing Pseudomonas aeruginosa. Results showed that X. pancheri leaf extract displayed antibacterial activity against S. aureus with higher inhibitory effect on MRSA compared to control gentamycin antibiotic, and IC50 value of 36.97 μg/mL [95%CI:29.59–46.18] against MRSA.
Kinetic Controlled Chirality Transfer and Induction in 2D Hydrogen‐Bonding Assemblies of Glycylglycine on Au(111)
Authors: Zhang Xin , Ding Haoxuan , Yang Shu , Yang Hualin , Yang Xiaoqing , Li Bosheng , Xing Xueting , Sun Yaojie , Gu Guangxin , Chen Xiaorui , Gao Jianzhi , Pan Minghu , Chi Lifeng , Guo Quanmin 
Published: January 2023

AbstractChirality transfer is of vital importance that dominates the structure and functionality of biological systems and living matters. External physical stimulations, e.g. polarized light and mechanical forces, can trigger the chirality symmetry breaking, leading to the appearance of the enantiomeric entities created from a chiral self‐assembly of achiral molecule. Here, several 2D assemblies with different chirality, synthesized on Au(111) surface by using achiral building blocks – glycylglycine (digly), the simplest polypeptide are reported. By delicately tuning the kinetic factors, i.e., one‐step slow/rapid deposition, or stepwise slow deposition with mild annealing, achiral square hydrogen‐bond organic frameworks (HOF), homochiral rhombic HOF and racemic rectangular assembly are achieved, respectively. Chirality induction and related symmetry broken in assemblies are introduced by the handedness (H‐bond configurations in principle) of the assembled motifs and then amplified to the entire assemblies via the interaction between motifs. The results show that the chirality transfer and induction of biological assemblies can be tuned by altering the kinetic factors instead of applying external forces, which may offer an in‐depth understanding and practical approach to peptide chiral assembly on the surfaces and can further facilitate the design of desired complex biomolecular superstructures.
Optimized fuzzy clustering‐based k‐nearest neighbors imputation for mixed missing data in software development effort estimation
Authors: Abnane Ibtissam , Idri Ali , Abran Alain 
Published: January 2023

AbstractContextSoftware development effort estimation (SDEE) is one of the most challenging aspects in project management. The presence of missing data (MD) in software attributes makes SDEE even more complex. K‐nearest neighbors imputation (KNNI) has been widely used in SDEE to deal with the MD issue. However, KNNI, in its classical process, has low tolerance to imprecision and uncertainty especially when dealing with categorical features. When dealing with categorical attributes, KNNI uses a classical approach, employing mainly numbers or classical intervals to represent software attributes and similarity measures originally designed for numerical attributes.ObjectivesThis paper evaluates the use of an optimized fuzzy clustering‐based KNNI (FC‐KNNI) and compares it with classical KNN when dealing with mixed data in the context of SDEE.MethodsWe investigate the effect of two imputation techniques (FC‐KNNI and KNNI) on five SDEE techniques: case‐based reasoning, fuzzy case‐based reasoning, support vector regression, multilayer perceptron, and reduced‐error pruning tree. The evaluation is carried out using six publicly available datasets for SDEE using two performance measures, standardized accuracy (SA), and Pred (0.25). The Wilcoxon statistical test is also performed to assess the significance of results.ResultsThe results are promising in the sense that using an imputation technique designed for mixed data is better than reusing methods originally designed for numerical data. We found that FC‐KNNI significantly outperforms KNNI regardless of the SDEE technique and dataset used. Another important finding is that F‐CBR improved the analogy process compared to CBR.ConclusionThe introduction of fuzzy sets and fuzzy clustering in the analogy process improves its performances in terms of SA and Pred (0.25).
Examining the training and education potential of the metaverse: Results from an empirical study of next generation SAFe training
Authors: Yilmaz Murat , O'Farrell Emer , Clarke Paul 
Published: January 2023

AbstractRestrictions imposed by the COVID‐19 pandemic have forced many to seek alternative means of training and learning, which ended up with increasing investment in the notion of the metaverse. Metaverse is envisioned as the next iteration of the Internet in which the virtual and the real world are blended to materialize a highly immersive experience. Not surprisingly, perhaps, next‐generation training and education systems are concerned with methods to integrate themselves into metaverse environments. In particular, participants are looking for more interactive and flexible training while maintaining a degree of educational content and high quality for their training plans and interactive workflows. In this paper, we conducted research to explore the role of metaverse in employee training. To this end, we utilized a variant of PlaySAFe (i.e., a 3D game) to investigate its metaverse adoption and usage. A qualitative design was adopted, using semistructured interviews to explore practitioners' experiences using the new version of PlaySAFe. After having it played in an industrial setting, we interviewed a group of software practitioners to compare the actual and expected features. This research has explored the pros and cons of using the current technologies for the practical groundwork of SAFe training. Findings from this research suggest that the metaverse holds the potential to deliver improved practical alignment in training and education programs, but that at the present time, practitioners expect more metaverse compatible features.
Hatchery supplementation increases potential spawning stock of Rio Grande Silvery Minnow after population bottlenecks
Authors: Archdeacon Thomas P. , Dudley Robert K. , Remshardt W. Jason , Knight William , Ulibarri Manuel , Gonzales Eric J. 
Published: January 2023

Abstract

Objective
Supplementation of imperiled wild fish stocks with captively raised fish is a commonly used conservation tool. Programs designed to maintain or improve fish populations through supplementation should be evaluated to determine whether they are meeting conservation objectives. The Rio Grande Silvery Minnow Hybognathus amarus is a small-bodied, endangered minnow endemic to the Rio Grande basin of the southwestern United States. The wild population of Rio Grande Silvery Minnow has been supplemented with captive-reared fish since 2002. Our objective was to determine whether supplementation measurably increases the number of spawning fish after years of population bottlenecks; this objective was simplified because nearly all hatchery-released fish have been given identifying markings.

Methods
We leveraged a long-term fish sampling data set (18 years) covering the species' contemporary range and a single-season, high spatial coverage fish sampling data set to determine whether the number of potential spawners was increased by the addition of hatchery fish.

Result
Hatchery-reared fish increased the catch rates of spawners by up to an order of magnitude in some years. We also observed that most hatchery-reared fish were recaptured near the point of release. We were able to determine that supplementation with hatchery fish increased the abundance and distribution of Rio Grande Silvery Minnow and provided the desired demographic boost after severe population bottlenecks.

Conclusion
Releasing hatchery-reared fish may be a useful tool for conservation of imperiled freshwater fishes. However, there is a need for spawning and rearing infrastructure that matches the life-history of the species and sufficient postrelease monitoring to evaluate the effectiveness of hatchery supplementation. Use of hatchery-reared fish has been beneficial for improving population resiliency of Rio Grande Silvery Minnow following recruitment bottlenecks; however, natural recruitment can greatly exceed hatchery supplementation and conditions promoting natural recruitment should be restored.
Transgenic RFP‐RPS‐30UbL strain of the nematode Caenorhabditis elegans as a biomonitor for environmental pollutants
Authors: Sun Weiwei , Liu Han , Qiao Aijun , Jiang Ting , Li Jianghui , Wang Long , Yang Ling , Huang Huicong , Yan Xiumei , Yan Baolong 
Published: January 2023

AbstractEnvironmental pollutants are recognized as one of the major concerns for public health. The free‐living nematode Caenorhabditis elegans are widely used to evaluate the toxicity of environmental contaminants in biomonitoring researches. In the present study, a new transgenic strain, rps‐30−/−;RFP‐RPS‐30UbL was generated, with constitutively active rps‐30 promoter used to control the expression of RFP‐RPS‐30UbL fusion protein. We found RFP‐RPS‐30UbL would accumulate to form ‘rod‐like’ structures, when worms were exposed to environmental contaminants, including Cd, Hg, Pb, As, Paraquat and Dichlorvos. The number of the ‘rod‐like’ structures was induced by environmental contaminants in a concentration‐ and time‐dependent manner. The ‘rod‐like’ structure formation could be detectable in response to the concentration of each contaminant as low as 24‐h LC50 × 10−7, and the detectable time could be within 2 h. Detecting the transcription and expression levels of RFP‐RPS‐30UbL in worms exposed to different kinds of environmental contaminants showed that the expression level of RFP‐RPS‐30UbL was not regulated by environmental contaminants, and the number differences of ‘rod‐like’ structures were just due to the morphological change of RFP‐RPS‐30UbL from dispersion to accumulation induced by environmental contaminants. In addition, this transgenic strain was developed in rps‐30−/− homozygous worm, which was a longevity strain. Detection of lifespan and brood size showed that rps‐30−/−;RFP‐RPS‐30UbL transgenic worm was more suitable to be cultured and used further than N2;GFP‐RPS‐30UbL, for expressing RPS‐30UbL in wild type N2 worms shortened the lifespan and deceased the brood size. Therefore, rps‐30−/−;RFP‐RPS‐30UbL transgenic worm might play a potential role in versatile environmental biomonitoring, with the advantage of not only the convenient and quick fluorescence‐based reporter assay, but also the quantificational evaluation of the toxicities of environmental contaminants using ‘rod‐like’ structures with high sensitivity, off‐limited the expression level of the reporter protein.