bluuebunny/crossref_metadata_2025_split huggingface dataset:
Epigenome-wide association study of diabetic chronic kidney disease progression in the Korean population: the KNOW-CKD study
Authors: Sung Hye Youn , Lee Sangjun , Han Miyeun , An Woo Ju , Ryu Hyunjin , Kang Eunjeong , Park Yong Seek , Lee Seung Eun , Ahn Curie , Oh Kook-Hwan , Park Sue K. , Ahn Jung-Hyuck 
Published: May 2023

AbstractSince the etiology of diabetic chronic kidney disease (CKD) is multifactorial, studies on DNA methylation for kidney function deterioration have rarely been performed despite the need for an epigenetic approach. Therefore, this study aimed to identify epigenetic markers associated with CKD progression based on the decline in the estimated glomerular filtration rate in diabetic CKD in Korea. An epigenome-wide association study was performed using whole blood samples from 180 CKD recruited from the KNOW-CKD cohort. Pyrosequencing was also performed on 133 CKD participants as an external replication analysis. Functional analyses, including the analysis of disease-gene networks, reactome pathways, and protein–protein interaction networks, were conducted to identify the biological mechanisms of CpG sites. A phenome-wide association study was performed to determine the associations between CpG sites and other phenotypes. Two epigenetic markers, cg10297223 onAGTR1and cg02990553 onKRT28indicated a potential association with diabetic CKD progression. Based on the functional analyses, other phenotypes (blood pressure and cardiac arrhythmia forAGTR1) and biological pathways (keratinization and cornified envelope forKRT28) related to CKD were also identified. This study suggests a potential association between the cg10297223 and cg02990553 and the progression of diabetic CKD in Koreans. Nevertheless, further validation is needed through additional studies.
Glycerol induced paraspinal muscle degeneration leads to hyper-kyphotic spinal deformity in wild-type mice
Authors: Noonan Alex M. , Buliung Emily , Briar K. Josh , Quinonez Diana , Séguin Cheryle A. , Brown Stephen H. M. 
Published: May 2023

AbstractDegenerative spinal disorders, including kyphotic deformity, are associated with a range of degenerative characteristics of the paraspinal musculature. It has therefore been hypothesized that paraspinal muscular dysfunction is a causative factor for degenerative spinal deformity; however, experimental studies demonstrating causative relationships are lacking. Male and female mice received either glycerol or saline injections bilaterally along the length of the paraspinal muscles at four timepoints, each separated by 2 weeks. Immediately after sacrifice, micro-CT was performed to measure spinal deformity; paraspinal muscle biopsies were taken to measure active, passive and structural properties; and lumbar spines were fixed for analysis of intervertebral disc (IVD) degeneration. Glycerol-injected mice demonstrated clear signs of paraspinal muscle degeneration and dysfunction: significantly (p < 0.01) greater collagen content, lower density, lower absolute active force, greater passive stiffness compared to saline-injected mice. Further, glycerol-injected mice exhibited spinal deformity: significantly (p < 0.01) greater kyphotic angle than saline-injected mice. Glycerol-injected mice also demonstrated a significantly (p < 0.01) greater IVD degenerative score (although mild) at the upper-most lumbar level compared to saline-injected mice. These findings provide direct evidence that combined morphological (fibrosis) and functional (actively weaker and passively stiffer) alterations to the paraspinal muscles can lead to negative changes and deformity within the thoracolumbar spine.
Aerosol demasking enhances climate warming over South Asia
Authors: Nair H. R. C. R. , Budhavant Krishnakant , Manoj M. R. , Andersson August , Satheesh S. K. , Ramanathan V. , Gustafsson Örjan 
Published: May 2023

AbstractAnthropogenic aerosols mask the climate warming caused by greenhouse gases (GHGs). In the absence of observational constraints, large uncertainties plague the estimates of this masking effect. Here we used the abrupt reduction in anthropogenic emissions observed during the COVID-19 societal slow-down to characterize the aerosol masking effect over South Asia. During this period, the aerosol loading decreased substantially and our observations reveal that the magnitude of this aerosol demasking corresponds to nearly three-fourths of the CO2-induced radiative forcing over South Asia. Concurrent measurements over the northern Indian Ocean unveiled a ~7% increase in the earth’s surface-reaching solar radiation (surface brightening). Aerosol-induced atmospheric solar heating decreased by ~0.4 K d−1. Our results reveal that under clear sky conditions, anthropogenic emissions over South Asia lead to nearly 1.4 W m−2heating at the top of the atmosphere during the period March–May. A complete phase-out of today’s fossil fuel combustion to zero-emission renewables would result in rapid aerosol demasking, while the GHGs linger on.
White spot syndrome virus (WSSV) modulates lipid metabolism in white shrimp
Authors: Ng Yen Siong , Cheng Cheng-Shun , Ando Masahiro , Tseng Yi-Ting , He Shu-Ting , Li Chun-Yuan , Cheng Shu-Wen , Chen Yi-Min , Kumar Ramya , Liu Chun-Hung , Takeyama Haruko , Hirono Ikuo , Wang Han-Ching 
Published: May 2023

AbstractIn addition to the Warburg effect, which increases the availability of energy and biosynthetic building blocks in WSSV-infected shrimp, WSSV also induces both lipolysis at the viral genome replication stage (12 hpi) to provide material and energy for the virus replication, and lipogenesis at the viral late stage (24 hpi) to complete virus morphogenesis by supplying particular species of long-chain fatty acids (LCFAs). Here, we further show that WSSV causes a reduction in lipid droplets (LDs) in hemocytes at the viral genome replication stage, and an increase in LDs in the nuclei of WSSV-infected hemocytes at the viral late stage. In the hepatopancreas, lipolysis is triggered by WSSV infection, and this leads to fatty acids being released into the hemolymph. β-oxidation inhibition experiment reveals that the fatty acids generated by WSSV-induced lipolysis can be diverted into β-oxidation for energy production. At the viral late stage, WSSV infection leads to lipogenesis in both the stomach and hepatopancreas, suggesting that fatty acids are in high demand at this stage for virion morphogenesis. Our results demonstrate that WSSV modulates lipid metabolism specifically at different stages to facilitate its replication.
A scalable sparse neural network framework for rare cell type annotation of single-cell transcriptome data
Authors: Cheng Yuqi , Fan Xingyu , Zhang Jianing , Li Yu 
Published: May 2023

AbstractAutomatic cell type annotation methods are increasingly used in single-cell RNA sequencing (scRNA-seq) analysis due to their fast and precise advantages. However, current methods often fail to account for the imbalance of scRNA-seq datasets and ignore information from smaller populations, leading to significant biological analysis errors. Here, we introduce scBalance, an integrated sparse neural network framework that incorporates adaptive weight sampling and dropout techniques for auto-annotation tasks. Using 20 scRNA-seq datasets with varying scales and degrees of imbalance, we demonstrate that scBalance outperforms current methods in both intra- and inter-dataset annotation tasks. Additionally, scBalance displays impressive scalability in identifying rare cell types in million-level datasets, as shown in the bronchoalveolar cell landscape. scBalance is also significantly faster than commonly used tools and comes in a user-friendly format, making it a superior tool for scRNA-seq analysis on the Python-based platform.
N6-methyladenosine helps Apostichopus japonicus resist Vibrio splendidus infection by targeting coelomocyte autophagy via the AjULK-AjYTHDF/AjEEF-1α axis
Authors: Liu Jiqing , Shao Yina , Li Dongdong , Li Chenghua 
Published: May 2023

AbstractN6-Methyladenosine (m6A) modification is one of the most abundant post-transcriptional modifications that can mediate autophagy in various pathological processes. However, the functional role of m6A in autophagy regulation is not well-documented during Vibrio splendidus infection of Apostichopus japonicus. In this study, the inhibition of m6A level by knockdown of methyltransferase-like 3 (AjMETTL3) significantly decreased V. splendidus-induced coelomocyte autophagy and led to an increase in the intracellular V. splendidus burden. In this condition, Unc-51-like kinase 1 (AjULK) displayed the highest differential expression of m6A level. Moreover, knockdown of AjULK can reverse the V. splendidus-mediated autophagy in the condition of AjMETTL3 overexpression. Furthermore, knockdown of AjMETTL3 did not change the AjULK mRNA transcript levels but instead decreased protein levels. Additionally, YTH domain-containing family protein (AjYTHDF) was identified as a reader protein of AjULK and promoted AjULK expression in an m6A-dependent manner. Furthermore, the AjYTHDF-mediated AjULK expression depended on its interaction with translation elongation factor 1-alpha (AjEEF-1α). Altogether, our findings suggest that m6A is involved in resisting V. splendidus infection via facilitating coelomocyte autophagy in AjULK-AjYTHDF/AjEEF-1α-dependent manner, which provides a theoretical basis for disease prevention and therapy in A. japonicus.
Chemistry for Sustainable Technologies: A Foundation
Authors: Winterton Neil 
Published: May 2023

Following the success of the first edition, this fully updated and revised book continues to provide an interdisciplinary introduction to sustainability issues in the context of chemistry and chemical technology. Its prime objective is to equip young chemists (and others) to more fully to appreciate, defend and promote the role that chemistry and its practitioners play in moving towards a society better able to control, manage and ameliorate its impact on the ecosphere. To do this, it is necessary to set the ideas, concepts, achievements and challenges of chemistry and its application in the context of its environmental impact, past, present and future, and of the changes needed to bring about a more sustainable yet equitable world.
Progress since 2010 is reflected by the inclusion of the latest research and thinking, selected and discussed to put the advances concisely in a much wider setting – historic, scientific, technological, intellectual and societal. The treatment also examines the complexities and additional challenges arising from public and media attitudes to science and technology and associated controversies and from the difficulties in reconciling environmental protection and global development.
While the book stresses the central importance of rigour in the collection and treatment of evidence and reason in decision-making, to ensure that it meets the needs of an extensive community of students, it is broad in scope, rather than deep. It is, therefore, appropriate for a wide audience, including all practising scientists and technologists.
Polymer Structure Characterization: From Nano to Macro Organization in Small Molecules and Polymers
Authors: Pethrick Richard A 
Published: May 2023

The different physical properties displayed by low molar mass organic materials and polymers are a result of their molecular organisation. In order to understand the structure – property relationship of a material it is necessary to first look at the interactions at a molecular level. This new edition of Polymer Structure Characterization provides readers with the background needed to understand the factors that influence molecular organization and how this affects the morphology and bulk physical properties of a material.
In order to introduce the concepts, the book first looks at small molecular systems and builds up to complex macromolecular systems. The second edition has been fully revised and updated to include new examples and references. Topics covered include: organic crystals, liquid crystals, plastic crystals, polymer crystal growth, amorphous glassy materials, polymer surfaces and interfaces, colloids and molecular organization in liquids as well as two new chapters on self-assembly and biopolymer systems.
The book is intended to provide complimentary material for a range of undergraduate and postgraduate courses in materials science, molecular chemistry and chemical physics. In addition to polymer and material scientists, the book would also be of interest to chemists and physicists studying the properties of organic materials.
Green Chemistry: Principles and Case Studies
Authors: Etzkorn Felicia A 
Published: May 2023

Green chemistry as a discipline is gaining increasing attention globally, with environmentally conscious students keen to learn how they can contribute to a safer and more sustainable world. Many universities now offer courses or modules specifically on green chemistry – Green Chemistry: Principles and Case Studies is an essential learning resource for those interested in mastering the subject.
Providing a comprehensive overview of the concepts of green chemistry this book engages students with a thorough understanding of what we mean by green chemistry and how it can be put into practice. Structured around the well-known 12 Principles, and firmly grounded in real-world applications and case-studies, this book shows how green chemistry is already being put into practice and prepare them to think about how they can be incorporated into their own work.
Targeted at advanced undergraduate and first-year graduate students with a background in general and organic chemistry, it is a useful resource both for students and for teachers looking to develop new courses.
Intelligibility of Word-Initial Obstruent Consonants in Mandarin-Speaking Prelingually Deafened Children With Cochlear Implants
Authors: Yang Jing , Wang Xianhui , Yu Jue , Xu Li 
Published: May 2023

Purpose:
This study assessed the intelligibility of obstruent consonants in prelingually deafened Mandarin-speaking children with cochlear implants (CIs).

Method:
Twenty-two Mandarin-speaking children with normal hearing (NH) aged 3.25–10.0 years and 35 Mandarin-speaking children with CIs aged 3.77–15.0 years were recruited to produce a list of Mandarin words composed of 17 word-initial obstruent consonants in different vowel contexts. The children with CIs were assigned to chronological age–matched (CA) and hearing age–matched (HA) subgroups with reference to the NH controls. One hundred naïve NH adult listeners were recruited for a consonant identification task that consisted of a total of 2,663 stimulus tokens through an online research platform. For each child speaker, the consonant productions were judged by seven to 12 different adult listeners. An average percentage of consonants correct was calculated across all listeners for each consonant.

Results:
The CI children in both the CA and HA subgroups showed lower intelligibility in their consonant productions than the NH controls. Among the 17 obstruents, both CI subgroups showed higher intelligibility for stops, but they demonstrated major problems with the sibilant fricatives and affricates and showed a different confusion pattern from the NH controls on these sibilants. Of the three places (alveolar, alveolopalatal, and retroflex) in Mandarin sibilants, both CI subgroups showed the lowest intelligibility and the greatest difficulties with alveolar sounds. For the NH children, there was a significant positive relationship between overall consonant intelligibility and chronological age. For the children with CIs, the best fit regression model revealed significant effects of chronological age and age at implantation, with their quadratic terms included.

Conclusions:
Mandarin-speaking children with CIs experience major challenges in the three-way place contrasts of sibilant sounds in consonant production. Chronological age and the combined effect of CI-related time variables play important roles in the development of obstruent consonants in the CI children.
120‐GHz 2‐bit reflection‐type phase shifter based on PIN diodes switched‐lines
Authors: Ouattara David , Durand Cédric , Bourdel Sylvain , Paillardet Frédéric , Vincent Loic , Corsi Jordan , Ferrari Philippe 
Published: May 2023

AbstractIn this paper, a 2‐bit digital reflection‐type phase shifter (RTPS) working at 120 GHz is presented. It uses a compact coupled‐lines coupler with low insertion loss and high isolation over a wide bandwidth. The loads are made by a microstrip‐line loaded by three PN jonction separated by an intermediate region I (PIN) diodes whose states are tuned ON/OFF to obtain 90°, 180° and 270° phase shift relative to the reference (0°). Measurement results show root mean square (RMS) phase and amplitude error equal to 10.3° and 1.2 dB, respectively. The maximum insertion loss is equal to 8.6 dB, leading to a figure of merit of 31°/dB. As shown by simulation, by flipping PIN diodes and using negative voltage for biasing, the maximum insertion loss could be reduced to 3.6 dB (figure of merit of 75°/dB) along with a great improvement in RMS phase and amplitude errors, thus showing the potential of the proposed architecture.
Multiple injections of energetic electrons associated with the flare and CME event on 9 October 2021
Authors: Jebaraj Immanuel C. , Kouloumvakos A. , Dresing N. , Warmuth A. , Wijsen N. , Palmroos C. , Gieseler J. , Marmyleva A. , Vainio R. , Krupar V. , Wiegelmann T. , Magdalenic J. , Schuller F. , Battaglia A. F. , Fedeli A. 
Published: May 2023

Context. We study the solar energetic particle (SEP) event observed on 9 October 2021 by multiple spacecraft, including Solar Orbiter. The event was associated with an M1.6 flare, a coronal mass ejection, and a shock wave. During the event, high-energy protons and electrons were recorded by multiple instruments located within a narrow longitudinal cone.
Aims. An interesting aspect of the event was the multi-stage particle energisation during the flare impulsive phase and also what appears to be a separate phase of electron acceleration detected at Solar Orbiter after the flare maximum. We aim to investigate and identify the multiple sources of energetic electron acceleration.
Methods. We utilised SEP electron observations from the Energetic Particle Detector (EPD) and hard X-ray (HXR) observations from the Spectrometer/Telescope for Imaging X-rays (STIX) on board Solar Orbiter, in combination with radio observations at a broad frequency range. We focused on establishing an association between the energetic electrons and the different HXR and radio emissions associated with the multiple acceleration episodes.
Results. We find that the flare was able to accelerate electrons for at least 20 min during the non-thermal phase, observed in the form of five discrete HXR pulses. We also show evidence that the shock wave contributed to the electron acceleration during and after the impulsive flare phase. The detailed analysis of EPD electron data shows that there was a time difference in the release of low- and high-energy electrons, with the high-energy release delayed. Also, the observed electron anisotropy characteristics suggest a different connectivity during the two phases of acceleration.
Smallholder Participation in Modernising Agri-Food Value Chains in Thailand: The Role of Traditional Markets
Authors: Ørtenblad Sinne Borby , Larsen Marianne Nylandsted , Bruun Thilde Bech , Ortiz-Gonzalo Daniel 
Published: May 2023

AbstractThe strategic focus of food retailers on stringent product requirements and the logistical management of fresh produce from farm to shelf have often been seen as raising barriers for smallholders, thus leading to the exclusion of resource-constrained farmers. Our study contributes to research on the inclusion of smallholders in modern agri-food value chains and on the integration of their perspectives in studies of dynamics in value chains through a combined qualitative and quantitative exploration of vegetable-producing smallholders in northern Thailand. We find that smallholders are not excluded from Thailand’s modern agri-food value chains, regardless of their household assets, but are instead integrated through traditional structures. To some extent, they do have room for manoeuvre in selecting different marketing channels and some bargaining power. Traditional markets can be very important for linking smallholders to modern agri-food value chains, but limited knowledge exchange, structural challenges in gaining access to certification and product differentiation all reduce the possibilities for upgrading. We emphasise the importance of viewing smallholders as active navigators within value chains and of understanding their motivations in making use of different sales channels in order to understand the complexities of their realities while not overlooking the power structures within value chains.
The sudden transition to remote learning in response to COVID-19: lessons from Malaysia
Authors: Salleh Mohd Idzwan Mohd , Alias Nor Aziah , Ariffin Suriyani , Ibrahim Zainuddin , Ramli Ahmad Razi , Aliman Sharifah 
Published: May 2023

AbstractHigher education students are frequently required to assess lecturers with a convenient, fast, and anonymous learning management system. Following the coronavirus disease 2019 (COVID-19) pandemic outbreak, Universiti Teknologi MARA Malaysia (UiTM) adopted a remote teaching and learning approach. This study examined how lecturers’ professionalism, course impression, and facilitating conditions at UiTM affected undergraduate and graduate students’ remote learning pre- and mid-pandemic. The higher prediction accuracy of the model demonstrated that students’ remote learning activities were highly related to lecturers’ professionalism, course impression, and facilitating conditions. The structural model demonstrated that the t-statistics of all measurement variables were significant at 1%. The strongest predictor of students’ enjoyment of remote learning pre- and mid-pandemic was lecturers’ professionalism. In the importance-performance matrix, lecturers’ professionalism was in the quadrant for ‘keep up the good work’. Facilitating conditions and course impression did not require further improvement even during the pandemic. The influence of remote learning was demonstrated in the students’ graduation rates and grades. The results also presented theoretical and practical implications for the UiTM hybrid learning plan post-pandemic.
The Literary-Inspired Web Series, Dickens and LGBTQIA+ Representation:Public History—A David Copperfield Web Series(2019–20)
Authors: Louttit Chris 
Published: May 2023

AbstractWeb series adaptations of the classics have been a familiar part of digital popular culture since the watershed appearance of The Lizzie Bennet Diaries (2012–13). Adaptation critics, including Douglas M. Lanier, Jennifer Camden, and Kate Faber Oestrich, have tended to focus on relatively high-profile Austen and Shakespeare vlog adaptations, especially those produced by Pemberley Digital. In this article, I update the genre’s genealogy by turning instead to later waves of low-budget creativity that have taken this transmedia form in new directions since the peak years of the literary-inspired web series in the mid-2010s. I pay particular attention to Quip Modest’s Public History–A David Copperfield Web Series (2019–20), a relatively rare example of a Dickens adaptation in the web series format which remoulds the multi-generational three-decker saga into an LGBTQIA+ positive coming-of-age narrative for the Generation Z audience. My contextualised close reading of the adaptation simultaneously reframes our understanding of Dickens’s digital canonicity and helps us better grasp the cultural logic of the literary web series adaptation community.