bluuebunny/crossref_metadata_2025_split huggingface dataset:
1H NMR‐based process understanding and biochemical marker identification methodology for monitoring CHO cell culture process during commercial‐scale manufacturing
Authors: Zhao Fang , Wan Yuxiang , Nie Lei , Jiao Jingyu , Gao Dong , Sun Yan , Chen Zhenhua , Shi Yingting , Yang Jiayu , Pan Jianyang , Wang Haibin , Qu Haibin 
Published: April 2023

AbstractControlling the process of CHO cell fed‐batch culture is critical for biologics quality control. However, the biological complexity of cells has hampered the reliable process understanding for industrial manufacturing. In this study, a workflow was developed for the consistency monitoring and biochemical marker identification of the commercial‐scale CHO cell culture process through 1H NMR assisted with multivariate data analysis (MVDA). Firstly, a total of 63 metabolites were identified in this study object in 1H NMR spectra of the CHO cell‐free supernatants. Secondly, multivariate statistical process control (MSPC) charts were used to evaluate process consistency. According to MSPC charts, the batch‐to‐batch quality consistency was high, indicating the CHO cell culture process at the commercial scale was well‐controlled and stable. Then, the biochemical marker identification was provided through orthogonal partial least square discriminant analysis (OPLS‐DA) based S‐line plots during the cell logarithmic expansion, stable growth, and decline phases. Identified biochemical markers of the three cell growth phases were as follows: L‐glutamine, pyroglutamic acid, 4‐hydroxyproline, choline, glucose, lactate, alanine, and proline were of the logarithmic growth phase; isoleucine, leucine, valine, acetate, and alanine were of the stable growth phase; acetate, glycine, glycerin, and gluconic acid were of the cell decline phase. Additional potential metabolic pathways that might influence the cell culture phase transitions were demonstrated. The workflow proposed in this study demonstrates that the combination of MVDA tools and 1H NMR technology is highly appealing to the research of the biomanufacturing process, and applies well to provide guidance in future work on consistency evaluation and biochemical marker monitoring of the production of other biologics.
Effect of chromosomal integration on catalytic performance of a multi‐component P450 system in Escherichia coli
Authors: Luelf U. Joost , Böhmer Lisa M. , Li Shengying , Urlacher Vlada B. 
Published: April 2023

AbstractCytochromes P450 are useful biocatalysts in synthetic chemistry and important bio‐bricks in synthetic biology. Almost all bacterial P450s require separate redox partners for their activity, which are often expressed in recombinant Escherichia coli using multiple plasmids. However, the application of CRISPR/Cas recombineering facilitated chromosomal integration of heterologous genes which enables more stable and tunable expression of multi‐component P450 systems for whole‐cell biotransformations. Herein, we compared three E. coli strains W3110, JM109, and BL21(DE3) harboring three heterologous genes encoding a P450 and two redox partners either on plasmids or after chromosomal integration in two genomic loci. Both loci proved to be reliable and comparable for the model regio‐ and stereoselective two‐step oxidation of (S)‐ketamine. Furthermore, the CRISPR/Cas‐assisted integration of the T7 RNA polymerase gene enabled an easy extension of T7 expression strains. Higher titers of soluble active P450 were achieved in E. coli harboring a single chromosomal copy of the P450 gene compared to E. coli carrying a medium copy pET plasmid. In addition, improved expression of both redox partners after chromosomal integration resulted in up to 80% higher (S)‐ketamine conversion and more than fourfold increase in total turnover numbers.
Impacts of hydrodynamic conditions and microscale surface roughness on the critical shear stress to develop and thickness of early‐stage Pseudomonas putida biofilms
Authors: Wei Guanju , Yang Judy Q. 
Published: April 2023

AbstractBiofilms can increase pathogenic contamination of drinking water, cause biofilm‐related diseases, alter the sediment erosion rate, and degrade contaminants in wastewater. Compared with mature biofilms, biofilms in the early‐stage have been shown to be more susceptible to antimicrobials and easier to remove. Mechanistic understanding of physical factors controlling early‐stage biofilm growth is critical to predict and control biofilm development, yet such understanding is currently incomplete. Here, we reveal the impacts of hydrodynamic conditions and microscale surface roughness on the development of early‐stage Pseudomonas putida biofilm through a combination of microfluidic experiments, numerical simulations, and fluid mechanics theories. We demonstrate that early‐stage biofilm growth is suppressed under high flow conditions and that the local velocity for early‐stage P. putida biofilms (growth time < 14 h) to develop is about 50 μm/s, which is similar to P. putida's swimming speed. We further illustrate that microscale surface roughness promotes the growth of early‐stage biofilms by increasing the area of the low‐flow region. Furthermore, we show that the critical average shear stress, above which early‐stage biofilms cease to form, is 0.9 Pa for rough surfaces, three times as large as the value for flat or smooth surfaces (0.3 Pa). The important control of flow conditions and microscale surface roughness on early‐stage biofilm development, characterized in this study, will facilitate future predictions and managements of early‐stage P. putida biofilm development on the surfaces of drinking water pipelines, bioreactors, and sediments in aquatic environments.
An adaptive modeling approach using spiking‐augmentation method to improve chemometric model performance in bioprocess monitoring
Authors: Zhao Yuxiang , Tang Yawen , Wasalathanthri Dhanuka , Xu Jianlin , Ding Julia 
Published: April 2023

AbstractIntensified and continuous processes require fast and robust methods and technologies to monitor product titer for faster analytical turnaround time, process monitoring, and process control. The current titer measurements are mostly offline chromatography‐based methods which may take hours or even days to get the results back from the analytical labs. Thus, offline methods will not meet the requirement of real time titer measurements for continuous production and capture processes. FTIR and chemometric based multivariate modeling are promising tools for real time titer monitoring in clarified bulk (CB) harvests and perfusate lines. However, empirical models are known to be vulnerable to unseen variability, specifically a FTIR chemometric titer model trained on a given biological molecule and process conditions often fails to provide accurate predictions of titer in another molecule under different process conditions. In this study, we developed an adaptive modeling strategy: the model was initially built using a calibration set of available perfusate and CB samples and then updated by augmenting spiking samples of the new molecules to the calibration set to make the model robust against perfusate or CB harvest of the new molecule. This strategy substantially improved the model performance and significantly reduced the modeling effort for new molecules.
Atractylodes Processing Products Protect against Gastric Ulcers in Rats by Influencing the NF‐κB‐MMP‐9/TIMP‐1 Regulatory Mechanism and Intestinal Flora
Authors: Zeng Linlin , Shen Ling , Fan Yilin , Luo Qiaomei , Hong Ran , Sun Xiaoli , Zhou Xia , Wan Jun 
Published: April 2023

AbstractAtractylodes macrocephala Koidz. (AM) is a Chinese herbal medicine that is widely used for treating gastrointestinal diseases. However, little research has focused on it as a single medicine for treating gastric ulcers. Honey‐bran stir‐frying is a characteristic method of concocting AM, so we speculated that AM is more effective after this preparation process. Analysis by ultra‐high‐performance liquid chromatography‐hybrid quadrupole‐Orbitrap high‐resolution mass spectrometry revealed changes in the chemical composition of raw Atractylodes (SG), bran‐fried Atractylodes (FG), and honey‐bran‐fried Atractylodes (MFG). MFG was superior to SG and FG in improving the pathological structure of gastric tissue in rats with acute gastric ulcers, reducing inflammatory cell infiltration in gastric tissue, and significantly reducing malondialdehyde while increasing superoxide dismutase and glutathione peroxidase, and reducing the damage caused by free radical accumulation in the gastric mucosa. In addition, MFG reduced the expression of matrix metalloproteinase‐9 (MMP‐9), an inhibitor of metalloproteinase‐1 (TIMP‐1) and nuclear factor kappa‐B (NF‐κB)proteins, inhibited inflammatory response, and regulated the degradation and rebalancing of the extracellular matrix. Fecal microbiota analysis also revealed that MFG normalized the intestinal flora to some extent. Our study shows that AM had a protective effect on rats with alcohol‐induced acute gastric ulcers before and after processing, and AM‐processed products were more effective than raw ones. Compared with MF, MFG had a higher rate of ulcer inhibition and a stronger anti‐inflammatory effect, and its mechanism of action was related to the NF‐κB‐MMP‐9/TIMP‐1 signaling pathway.
The impact of a statewide payment reform on transcatheter aortic valve replacement (TAVR) utilization and readmissions
Authors: Yesantharao Pooja S. , Etchill Eric W. , Zhou Alice L. , Ong Chin Siang , Metkus Thomas S. , Canner Joseph K. , Alejo Diane E. , Aliu Oluseyi , Czarny Matthew J. , Hasan Rani K. , Resar Jon R. , Schena Stefano 
Published: April 2023

AbstractBackgroundTranscatheter aortic valve replacement (TAVR) is an increasingly used but relatively expensive procedure with substantial associated readmission rates. It is unknown how cost‐constrictive payment reform measures, such as Maryland's All Payer Model, impact TAVR utilization given its relative expense. This study investigated the impact of Maryland's All Payer Model on TAVR utilization and readmissions among Maryland Medicare beneficiaries.MethodsThis was a quasi‐experimental investigation of Maryland Medicare patients undergoing TAVR between 2012 and 2018. New Jersey data were used for comparison. Longitudinal interrupted time series analyses were used to study TAVR utilization and difference‐in‐differences analyses were used to investigate post‐TAVR readmissions.ResultsDuring the first year of payment reform (2014), TAVR utilization among Maryland Medicare beneficiaries dropped by 8% (95% confidence interval [CI]: −9.2% to −7.1%; p < 0.001), with no concomitant change in TAVR utilization in New Jersey (0.2%, 95% CI: 0%–1%, p = 0.09). Longitudinally, however, the All Payer Model did not impact TAVR utilization in Maryland compared to New Jersey. Difference‐in‐differences analyses demonstrated that implementation of the All Payer Model was not associated with significantly greater declines in 30‐day post‐TAVR readmissions in Maryland versus New Jersey (−2.1%; 95% CI: −5.2% to 0.9%; p =0.1).ConclusionsMaryland's All Payer Model resulted in an immediate decline in TAVR utilization, likely a result of hospitals adjusting to global budgeting. However, beyond this transition period, this cost‐constrictive reform measure did not limit Maryland TAVR utilization. In addition, the All Payer Model did not reduce post‐TAVR 30‐day readmissions. These findings may help inform expansion of globally budgeted healthcare payment structures.
Possibilities of Optimizing the Purity of Mannose in Continuous Chromatographic Separation
Authors: Gillarová Simona , Henke Svatopluk , Svoboda Tomáš , Krčová Pavla , Bubník Zdeněk , Kadlec Pavel , Sluková Marcela 
Published: April 2023

AbstractThe development of separation technology based on continuous simulated moving bed chromatography in sugar technology is described. This is a potential economic alternative method both in terms of separation efficiency and process performance, and in terms of the high purity of the resulting products. This work focuses in detail on the isolation of mannose from natural polysaccharides, such as galactomannans; however, the developed procedures and equipment can be further applied to the separation of various liquid mixtures of carbohydrates. The main aim was to find optimal operating conditions for hydrolytic and separation steps of the whole technology. The obtained results provide a basic data set for further development of the industrial application.
Personality and wealth
Authors: Fenton‐O'Creevy Mark , Furnham Adrian 
Published: April 2023

AbstractTo what extent do personality traits predict wealth in adulthood over and above standard demographic factors? In all 3240 adults in the UK completed a Big Five personality test and reported on their property wealth, savings and investments, and their physical valuable items. We also had data on their age, education, household income and gender. Correlations and regressions showed that the demographics, particularly age and income were, as expected clearest correlates of wealth. Conscientious was positively and agreeableness, neuroticism and extraversion were negatively associated with savings and investments. The data pointed clearly to conscientiousness as the most important personality trait in wealth accumulation. Implications of these results as well as limitations of the study are discussed.
Cu2P7‐CoP Heterostructure Nanosheets Enable High‐Performance of 5‐Hydroxymethylfurfural Electrooxidation
Authors: Bi Jiahui , Xu Hui , Wang Wenke , Sang Ting , Jiang Anning , Hao Jingcheng , Li Zhonghao 
Published: April 2023

AbstractElectrooxidation of 5‐hydroxymethylfural (HMF) into 2,5‐furandicarboxylicacid (FDCA) has been regarded as a promising sustainable approach to achieve value‐added chemicals. However, it is still impeded by the unsatisfactory performance of electrocatalysts. Here, Cu2P7‐CoP heterostructure nanosheets were reported to enable powerful HMF electrooxidation. The Cu2P7‐CoP heterostructure nanosheets were fabricated by microwave‐assisted deep eutectic solvent (DES) approach, along with subsequent phosphiding. The Cu2P7‐CoP heterostructure nanosheets enabled a superb 100 % HMF conversion at 1.43 V (vs. RHE) with 98.8 % FDCA yield and 98 % Faradaic efficiency (FE), demonstrating its promising application in HMF electrooxidation. X‐ray photoelectron spectroscopy (XPS) analysis, open‐circuit potential (OCP) approach and density functional theory (DFT) calculation uncovered that the electron transfer and redistribution between Cu2P7 and CoP improved the adsorption capacity of HMF and modulated the catalytic performance. This study not only offered a powerful electrocatalyst for HMF electrooxidation, but also provided a conceptually new strategy for the heterostructure catalyst design.
Wide zero‐voltage switching range full‐bridge converter based on adjustable parallel current
Authors: Gao Yahu , Tang Yu , Wang Hongchang , Zhao Zixiang , Wang Guanghui , Song Yinghao 
Published: April 2023

AbstractAiming at the problems of narrow ZVS range, large duty cycle loss, and severe voltage oscillation of phase‐shift‐controlled full‐bridge converters, this paper proposes a converter that realizes ZVS by adjusting the parallel current. No additional series inductor is required, avoiding large duty cycle loss and weakening voltage oscillations on the secondary side. The converter with small duty cycle loss can use a transformer with a larger turns ratio, which can reduce the voltage stress of the secondary diodes and the filter inductor current ripple. The parallel current required to realize ZVS is analyzed theoretically considering the influence of leakage inductor energy on ZVS. A comparative experiment between the proposed converter and the conventional full‐bridge converter is carried out to verify the performance of the proposed converter.
Environmental variables and species traits as drivers of wild bee pollination in intensive agroecosystems—A metabarcoding approach
Authors: Querejeta Marina , Marchal Lorène , Pfeiffer Paul , Roncoroni Marilyn , Bretagnolle Vincent , Gaba Sabrina , Boyer Stéphane 
Published: April 2023

AbstractWild bees are known to be efficient pollinators of wild plants and cultivated crops and they are essential ecosystem service providers. However, wild bee populations have been suffering from significant declines in the last decades mainly due to the use of agrochemicals. Within this framework, we aimed to characterize wild bees' pollination spectrum (i.e., the community of pollinated flowering plants) in intensive agroecosystems, and describe the environmental variables and wild bee species traits influencing the pollination. To do this, we conducted metabarcoding analyses of pollen loads from wild bees collected in sunflower crops in the French region of Nouvelle‐Aquitaine. Our study revealed that wild bees visited flowering plants corresponding to 231 different operational taxonomic units, classified into 38 families of which Asteraceae, Brassicaceae, and Apiaceae were the most visited and more than 90% of the visited taxa turned out to be wildflowers. We also analyzed the potential effect of environmental variables and wild bee species traits in governing their choice of pollinated plants. The community composition of pollinated plants varied depending on the flowering stages of the sunflower and the farming system. Our results also show that pollination niche breadth (alpha diversity) varied depending on the flowering stages of the sunflower but was not different between organic and conventional farming systems. Regarding wild bee species traits, the community composition of pollinated plants varied in relation to wild bees' body sizes and sociality levels. Our results are consistent with previous studies, suggesting that solitary bees are more specialist when it comes to flower selection than social bees, which are more generalist. The metabarcoding of pollen loads enabled us to draw a global picture of plant–wild bee interactions in an intensive agroecosystem. Our findings support the hypothesis that a higher diversity of weeds may increase wild bee diversity in intensive agroecosystems.
A Light‐Induced Decarboxylative‐Elimination of Substituted Maleimides as a Strategy Towards Triggered Photorelease
Authors: Malde Roshni , Richards Daniel A. , Salerno Luigia , Zhao Yanbo , Karu Kersti , Chudasama Vijay , Baker James R. 
Published: April 2023

AbstractHerein we report on photodecarboxylations of various substituted maleimides, resulting in an elimination reaction. Furthermore, we establish facile wavelength tunability through modulation of the maleimide double bond substituents. We envisage that these versatile reagents, which are readily constructed and diversified by nucleophilic substitution reactions on bromomaleimides, will offer new opportunities for triggered photorelease.
Evaluation of the solidification process in a double‐tube latent heat storage unit equipped with circular fins with optimum fin spacing
Authors: Bahlekeh Abdullah , Mouziraji Hosseinali Ramezani , Togun Hussein , Tiji Abolfazl Ebrahimnataj , Abed Azher M. , Mohammed Hayder I. , Ibrahem Raed Khalid , Alhassan Muataz S. , Talebizadehsardari Pouyan 
Published: April 2023

AbstractIn this study, the effect of fin number and size on the solidification output of a double‐tube container filled with phase change material (PCM) was analyzed numerically. By altering the fins' dimensions, the PCM's heat transfer performance is examined and compared to finless scenarios. To attain optimal performance, multiple inline configurations are explored. In addition, the initial conditions of the heat transfer fluid (HTF), including temperature and Reynolds number, are considered in the analysis. The research results show a significant impact of longer fins with higher numbers on improving the solidification rate of PCM. The solidification rate increases by 67%, 170%, 308%, and 370% for cases with 4, 9, 15, and 19 fins, respectively, all with the same fin length and initial HTF boundary condition. The best case results in a solidification time that is 4.45 times shorter compared to other fin number and dimension scenarios. The study also found that moving from Reynolds numbers 500 to 1000 and 2000 reduced discharging times by 12.9% and 22%, respectively, and increased heat recovery rates by 14.4% and 27.9%. When the HTF entrance temperature was 10°C and 15°C, the coolant temperature showed that the entire discharging time decreased by 37.5% and 23.1% relative to the solidification time when the initial temperature was 20°C. Generally, this work highlights that increasing the length and number of fins enhances thermal efficiency and the phase change process.
Progress on rock thermal energy storage (RTES): A state of the art review
Authors: Amiri Leyla , Ermagan Hamidreza , Kurnia Jundika C. , Hassani Ferri , Sasmito Agus P. 
Published: April 2023

AbstractThermal energy is one of the most widely encountered energy forms in our daily life. To ensure efficient utilization and conversion of this energy, the balance between supply and demand needs to be maintained. For this purpose, thermal energy storage is required. There are various thermal energy storage systems available; one of the most basic is sensible thermal energy storage which includes rock thermal energy storage (RTES). This rock‐based energy storage has recently gained significant attention due to its capability to hold large amounts of thermal energy, relatively simple storage mechanism and low cost of storage medium. Accordingly, numerous studies have been conducted to elucidate the basic flow and heat transfer mechanism and to evaluate the performance of this energy storage. The major technical challenges hindering the wide adoption of this technology are the enormous pressure drop across the storage and nonoptimal heat transfer from the heat transfer fluid to the storage medium and vice versa. These issues will directly and indirectly affect the overall cost (capital, operational, and maintenance costs) of the system. To eliminate this issue and assist further development of this technology, it is crucial to compile and extract important findings from these previous studies, identify the challenge and research gap, and draw guidelines for the upcoming research and development. At the moment, this kind of compilation does not exist. Hence, this paper is prepared with the primary objective to comprehensively review the current technology and development of RTES and to propose a potential way forward based on the pain point identified. Discussion on the nontechnical aspect such as policy and regulations as well as community awareness will also be outlined and discussed.