bluuebunny/crossref_metadata_2025_split huggingface dataset:
CRNDE, an enhancer RNA of prognostic value in glioma, correlates with immune infiltration: A pan-cancer analysis
Authors: Huo Zheng , Guo LiShuo , Shao Wei , Ding Qian , Guo Yue , Xu Qian 
Published: March 2023

Background: Recent evidence has emerged regarding the modification of the relationship between the enhancer RNA (eRNA) colorectal neoplasia differentially expressed (CRNDE) and the prognosis of patients with cancer. Nevertheless, the role of CRNDE in pan-cancer and tumour immune microenvironment (TIME) remains largely unexplored. Methods: Multiple bioinformatic methods were employed to unravel the role of elncRNA CRNDE in a wide range of cancers from the perspective of the expression patterns, prognosis, tumour immunity, and the association between CRNDE and its target gene IRX5 based on the public database. A nomogram was created by integrating independent factors. Gene set enrichment analysis (GSEA) was used to determine the potential signalling pathway mechanisms. Molecular biology experiments including RT-qPCR, MTT, Transwell, and wound-healing analyses were performed to evaluate the proliferation, migration, and invasion of glioma cells. Finally, RT-qPCR and immunohistochemistry were performed to analyse the expression and function of CRNDE in gliomas of different grades. Nissl staining was used to evaluate the potential effects of CRNDE knockdown in glioblastoma tissues. Results: CRNDE was dysregulated and associated with clinical features in 15 of the 33 cancer types. The survival analysis showed that high CRNDE expression might lead to a worsened prognosis among the six cancer types. CRNDE might be involved in immune regulation in multiple cancer types. CRNDE correlated with multiple tumours and immune-related pathways. Additionally, CRNDE knockdown inhibited glioma cell proliferation, migration, and invasion. Nissl staining confirmed that CRNDE siRNA notably decreased tumour growth. A stronger correlation between immune cells and CRNDE/IRX5 in lower-grade glioma than in glioblastoma multiforme tissues, and an association with IRX5 and immune checkpoint gene NRP1 were observed. Conclusions: An Enhancer RNA CRNDE may serve as a biomarker for cancer immunologic infiltration and poor prognosis, providing a new approach for cancer treatment.
Multifactorial Exercise Intervention Decreases Falls Risk in High-risk and Low-risk Older Adults
Authors: Estep Amanda , Morrison Steven , Caswell Shane V. , Ambegaonkar Jatin P. , Vaz João R , Cortes Nelson 
Published: March 2023

Background: Each year, 1 in 4 people over the age of 65 years of age will experience a fall. It is important to identify and address modifiable risk factors that are associated with falls in adults at high and low risk for falls. Hypothesis: Falls risk improves in both high-risk and low-risk participants with the implementation of Stay Active and Independent for Life (SAIL). Study Design: Cohort study Level of Evidence: Level 3. Methods: Seventy-eight older adults (age, 70.9 ± 5.1 years) were included in this study and categorized into high risk and low risk for falling based on the falls risk score from the Physiological Profile Assessment. High risk was defined as having a preintervention falls risk score >1, whereas low risk was defined as having a preintervention falls risk score <1. Both groups had the same 10-week intervention. A multivariate analysis of covariance was used to compare differences pre- and postintervention, using preintervention falls risk score as covariate. Results: Results showed that regardless of preintervention falls risk, participants showed significant improvements in right and left knee extensor strength and sit-to-stand after participation in the 10-week SAIL program. Also, noteworthy is that 15 participants who were considered at high risk for falling preintervention were considered low risk for falling postintervention. Conclusion: The positive outcomes noted on modifiable risk factors suggest SAIL can be beneficial for decreasing falls risk in older adults, regardless of risk of falling, using a multifactorial exercise intervention. Our results also showed that it was possible for participants not only to improve falls risk but to improve to such a degree that they change from high risk to low risk of falling. Clinical Relevance: Our results demonstrated that SAIL was effective in improving overall fall risk after a 10-week intervention. Targeted community-based interventions for the aging population can bring physical health benefits that can decrease falls risk.
Inpatient Mortality in Parkinson’s Disease
Authors: Phillips Oliver W. , Kunicki Zachary , Jones Richard , Belanger Emmanuelle , Shireman Theresa I. , Friedman Joseph H. , Kim Duk Soo , Kluger Benzi , Akbar Umer 
Published: March 2023

Introduction Although a majority of the American public prefer to die at home, a large percentage of Parkinson’s disease patients die in acute care hospitals. We examine trends in the clinical and demographic characteristics of Parkinson’s disease patients who die in a hospital to identify populations potentially vulnerable to unwanted inpatient mortality. Methods Patients with Parkinson’s disease admitted to a hospital from 2002-2016 were identified from the National Inpatient Sample (n = 710,013) along with their associated clinical and demographic characteristics. The main outcome examined was mortality during inpatient admission. From these data, logistic regression models were estimated to obtain the odds ratios of inpatient mortality among clinical and demographic attributes, and their change over time. Results Characteristics significantly associated with increased odds of inpatient mortality included increased age (OR = 1.70 for 55-65, 2.52 for 66-75, 3.99 for 76-85, 5.72 for 86+, all P < 0.001), length of stay ≤5 days (reference; 6 + days OR = 0.37, P < 0.001), white race or ethnicity (reference; Black OR = .84 P < .001, Hispanic OR = 0.91 P = 0.01), male (reference; female OR = 0.93 P < 0.001), hospitalization in Northeast (reference; Midwest OR = 0.78, South 0.84, West OR = 0.82; all P < 0.001), higher severity of illness (moderate OR = 1.50, major OR = 2.32, extreme OR = 5.57; all P < 0.001), and mortality risk (moderate OR = 2.88, major OR = 10.92, extreme OR = 52.30; all P < 0.001). Fitted probabilities overall declined over time. Conclusion Differences exist among PD patient populations regarding likelihood of in-hospital mortality that are changing with time. Insight into which PD patients are most at risk for inpatient mortality may enable clinicians to better meet end-of-life care needs.
Self-Efficacy and Teaching Effectiveness
Authors: Barnes Gail V. 
Published: March 2023

Interpreting changing levels of self-efficacy while improving effective teaching behaviors can be one goal of teacher education programs. Undergraduate education majors often begin their teacher education programs with high levels of self-efficacy (a belief in one's capabilities). These levels of self-efficacy frequently decline as preservice teachers progress through their curriculum and make the transition to in-service teaching. A slight decline in these levels can be interpreted as a novice teacher's greater understanding of the complexity of the teaching process. Eighteen preservice string teachers evaluated their levels of self-efficacy with the Teacher Efficacy Scale (Guskey and Passaro, 1994) three times during an academic year. The preservice teachers were videotaped three times during regular teaching assignments at a community music program. They self-evaluated the teaching episodes by the means of a Music Teaching Observation Form (Kelly, 1984). Experienced educators also evaluated the tapes. Group and individual means indicated that ratings of teaching effectiveness by both preservice teachers and experienced educators increased while levels of self-efficacy decreased slightly. Several specific items from the Teacher Efficacy Scale and Music Teaching Teaching Observation Form had significant changes. Changing levels in self-efficacy did not have an influence on increased teaching effectiveness scores for this specific group of preservice teachers.
A solvent-free process to design low density polyhydroxyalkanoate
Authors: De Carvalho Eva , Chebil Mohamed , Fay Fabienne , Renard Estelle , Langlois Valérie 
Published: March 2023

The polyhydroxyalkanoates (PHAs) are biobased, biocompatible and biodegradable polyesters. To enhance their biodegradability and flexibility, we develop low density PHAs by an emulsion-templated method without organic solvent. Polycaprolactone (PCL) and castor oil are required to improve the flexibility and to generate the porosity, respectively. To overcome the well-known lack of compatibility between PHA and PCL, pluronic is introduced to improve the miscibility of these polyesters The densities of native PHA and PCL are 1.2 g.cm−3 and the densities decreased to 0.49 g.cm−3 in presence of castor oil (40 wt%). Different PHAs were studied: polyhydroxybutyrate (PHB), polyhydroxybutyrate-co-hydroxyvalerate (PHBHV) and polyhydroxybutyrate-co-hydroxyhexanoate (PHBHHx). The porous PHBHHx based material showed the best colonization by Pseudomonas, followed by the PHBHV and PHB. This colonization only occurred in the surface and no bacterial diffusion was observed inside the material. The biodegradability study in presence of lipase showed that after 8 days, the weight losses are, respectively, 20% and 75% when the densities decreased from 0.82 g.cm−3 to 0.49 g.cm−3. These results showed the importance of the porosity on the biodegradation of PHAs.
Clinical prediction rule for bacterial arthritis: Chi-squared automatic interaction detector decision tree analysis model
Authors: Kushiro Seiko , Fukui Sayato , Inui Akihiro , Kobayashi Daiki , Saita Mizue , Naito Toshio 
Published: March 2023

Objectives: Differences in demographic factors, symptoms, and laboratory data between bacterial and non-bacterial arthritis have not been defined. We aimed to identify predictors of bacterial arthritis, excluding synovial testing. Methods: This retrospective cross-sectional survey was performed at a university hospital. All patients included received arthrocentesis from January 1, 2010, to December 31, 2020. Clinical information was gathered from medical charts from the time of synovial fluid sample collection. Factors potentially predictive of bacterial arthritis were analyzed using the Student’s t-test or chi-squared test, and the chi-squared automatic interaction detector decision tree analysis. The resulting subgroups were divided into three groups according to the risk of bacterial arthritis: low-risk, intermediate-risk, or high-risk groups. Results: A total of 460 patients (male/female = 229/231; mean ± standard deviation age, 70.26 ± 17.66 years) were included, of whom 68 patients (14.8%) had bacterial arthritis. The chi-squared automatic interaction detector decision tree analysis revealed that patients with C-reactive protein > 21.09 mg/dL (incidence of septic arthritis: 48.7%) and C-reactive protein ⩽ 21.09 mg/dL plus 27.70 < platelet count ⩽ 30.70 × 104/μL (incidence: 36.1%) were high-risk groups. Conclusions: Our results emphasize that patients categorized as high risk of bacterial arthritis, and appropriate treatment could be initiated as soon as possible.
Association Between Dental Infection and Increased Incidence of Complications in Sickle Cell Disease Children: A Cross-sectional Study in Jeddah City
Authors: Felemban Osama , Alhalees Lama , Alattas Logain , Baghlaf Khlood , Aldajani Mariam 
Published: March 2023

Aim: Children with sickle cell disease (SCD) are prone to many complications. However, little is known about oral infectious. This study aimed to assess the oral health status of pediatric patients with SCD and explore the associations between oral health conditions and clinical complications of SCD. Materials and Methods: A cross-sectional study was conducted in children with SCD admitted to the pediatric wards of two government hospitals in Jeddah city. The children were examined for dental caries, gingival diseases, oral lesions, and dental infections. Oral findings were compared between children who were hospitalized for vaso-occlusive crisis (VOC) or had a history of VOC in the last 6 or 12 months and those who were admitted for other reasons such as blood transfusion or other complications or had no history of VOC in the last 6 or 12 months. Results: A total of 107 children with SCD were included, with a mean age of 9.82 ± 3.82 years, of which 51 (47.7%) were females. Of the total, 58 (54.2%) were admitted because of VOC. There was a significant relationship between VOC and dental attrition ( P = 0.038), osteomyelitis, and dental abscesses ( P = 0.004). A significant association was found between the presence of dental abscesses and a history of osteomyelitis or avascular necrosis of long bones in the previous 12 months ( P = 0.001 and P = 0.041, respectively). Conclusion: Dental abscesses and non-carious lesions are likely to be associated with VOC; however, further prospective cohort studies are warranted to confirm this association and establish a causal relationship.
Health Conditions and Tooth Loss in the Population of Brazil: National Health Survey (2013 and 2019)
Authors: Lima Erica Nobre , Araújo Larissa Fortunato , Florêncio Caroline Mary Gurgel Dias 
Published: March 2023

Aim: Certain systemic risk factors associated with tooth loss are much more common. However, population-based studies that have investigated the impact of these diseases on tooth loss and age groups are not found. The study aimed to evaluate the relationship and change in effect by age in systemic diseases with the loss of 13 or more teeth in adults and elderly people in Brazil, comparing two periods, 2013 and 2019. Materials and Methods: A total of 49,553 participants aged 18 years or older from the National Health Survey in 2013 and 77,862 in 2019 were used. Data was sorted out into five different age groups. Using a Poisson Regression model, the magnitudes of associations were analysed with sequential adjustment (95% CI). Results: All diseases (Arterial hypertension, Rheumatoid Arthritis, Diabetes, Depression and Hypercholesterolemia) were associated with tooth loss (statistically significant magnitudes of associations) in the total population in 2013 and 2019. The magnitudes of associations were decreasing in older age groups and it was only statistically significant with cardiovascular diseases and tooth loss in ≥ 65-year age group (1.10 (95% CI:1.05–1.15)). Conclusion: More than 13 teeth loss were associated with Arthritis/rheumatism, Depression, Diabetes Mellitus, Hypercholesterolemia, Systemic Arterial Hypertension and cardiovascular diseases, especially among younger age groups, which suggests a focus on the prevention and control of such systemic diseases and primary dental care.
The shape-retention and heat-tolerant elastic helix based on azobenzene-polyimide supramolecular assembly
Authors: Li Shuangwen , Cui Yongsheng , Jia Shuai , Lin Song , Gong Lan , An Haoran , Sun Yanrong , Xu Linan 
Published: March 2023

Helical structures are ubiquitous in natural and engineered systems across multiple length scales, while they often exhibit nearly uniform radius and pitch. Utilization of biomimetic structure design to develop stretchable and robust 3D helical structures that are shape-preserving and heat-tolerant is of great interest. Herein, we devise flexible 3D helixes by supramolecular self-assembly and high voltage electric field orientation of molecular chains. The 3D helixes not only inherit buffering mechanics with exceptional shape-retention ability against cyclic stretching but also exhibit excellent heat-resistant quality. The results of the experimental tests confirm that the helix can recover its original shape without obvious residual strain and basically withstand after 2000 cycles under force loading of 10 cN. Such robust biomimetic materials hold great prospects in the fields of artificial muscles, wearable materials, and so on.
Molecular associations of response to the new-generation BTK inhibitor zanubrutinib in marginal zone lymphoma
Authors: Tatarczuch Maciej , Waltham Mark , Shortt Jake , Polekhina Galina , Hawkes Eliza A. , Ho Shir-Jing , Trotman Judith , Brasacchio Daniella , Co Melannie , Li Jessica , Ramakrishnan Vanitha , Dunne Karin , Opat Stephen S. , Gregory Gareth P. 
Published: March 2023

Abstract
Using tissue whole exome sequencing (WES) and circulating tumor cell–free DNA (ctDNA), this Australasian Leukaemia & Lymphoma Group translational study sought to characterize primary and acquired molecular determinants of response and resistance of marginal zone lymphoma (MZL) to zanubrutinib for patients treated in the MAGNOLIA clinical trial. WES was performed on baseline tumor samples obtained from 18 patients. For 7 patients, ctDNA sequence was interrogated using a bespoke hybrid-capture next-generation sequencing assay for 48 targeted genes. Somatic mutations were correlated with objective response data and survival analysis using Fisher exact test and Kaplan-Meier (log-rank) method, respectively. Baseline WES identified mutations in 33 of 48 (69%) prioritized genes. NF-κB, NOTCH, or B-cell receptor (BCR) pathway genes were implicated in samples from 16 of 18 patients (89%). KMT2D mutations (n = 11) were most common, followed by FAT1 (n = 9), NOTCH1, NOTCH2, TNFAIP3 (n = 5), and MYD88 (n = 4) mutations. MYD88 or TNFAIP3 mutations correlated with improved progression-free survival (PFS). KMT2D mutations trended to worse PFS. Acquired resistance mutations PLCG2 (R665W/R742P) and BTK (C481Y/C481F) were detected in 2 patients whose disease progressed. A BTK E41K noncatalytic activating mutation was identified before treatment in 1 patient who was zanubrutinib-refractory. MYD88, TNFAIP3, and KMT2D mutations correlate with PFS in patients with relapsed/refractory MZL treated with zanubrutinib. Detection of acquired BTK and PLCG2 mutations in ctDNA while on therapy is feasible and may herald clinical disease progression. This trial was registered at https://anzctr.org.au/ as #ACTRN12619000024145.
Genome Scan of Rice Landrace Populations Collected Across Time Revealed Climate Changes’ Selective Footprints in the Genes Network Regulating Flowering Time
Authors: Ahmadi Nourollah , Barry Mamadou Billo , Frouin Julien , de Navascués Miguel , Toure Mamadou Aminata 
Published: March 2023

AbstractAnalyses of the genetic bases of plant adaptation to climate changes, using genome-scan approaches, are often conducted on natural populations, under hypothesis of out-crossing reproductive regime. We report here on a study based on diachronic sampling (1980 and 2011) of the autogamous crop species, Oryza sativa and Oryza glaberrima, in the tropical forest and the Sudanian savannah of West Africa. First, using historical meteorological data we confirmed changes in temperatures (+ 1 °C on average) and rainfall regime (less predictable and reduced amount) in the target areas. Second, phenotyping the populations for phenology, we observed significantly earlier heading time in the 2010 samples. Third, implementing two genome-scan methods (one of which specially developed for selfing species) on genotyping by sequencing genotypic data of the two populations, we detected 31 independent selection footprints. Gene ontology analysis detected significant enrichment of these selection footprints in genes involved in reproductive processes. Some of them bore known heading time QTLs and genes, including OsGI, Hd1 and OsphyB. This rapid adaptive evolution, originated from subtle changes in the standing variation in genetic network regulating heading time, did not translate into predominance of multilocus genotypes, as it is often the case in selfing plants, and into notable selective sweeps. The high adaptive potential observed results from the multiline genetic structure of the rice landraces, and the rather large and imbricated genetic diversity of the rice meta-population at the farm, the village and the region levels, that hosted the adaptive variants in multiple genetic backgrounds before the advent of the environmental selective pressure. Our results illustrate the evolution of in situ diversity through processes of human and natural selection, and provide a model for rice breeding and cultivars deployment strategies aiming resilience to climate changes. It also calls for further development of population genetic models for adaptation of plant populations to environmental changes. To our best knowledge, this is the first study dealing with climate-changes’ selective footprint in crops.
Validation with single-step SNPBLUP shows that evaluations can continue using a single mean of genotyped individuals, even with multiple breeds
Authors: Aldridge Michael , Vandenplas Jeremie , Duenk Pascal , Henshall John , Hawken Rachel , Calus Mario 
Published: March 2023

Abstract
Background
In genomic prediction, it is common to centre the genotypes of single nucleotide polymorphisms based on the allele frequencies in the current population, rather than those in the base generation. The mean breeding value of non-genotyped animals is conditional on the mean performance of genotyped relatives, but can be corrected by fitting the mean performance of genotyped individuals as a fixed regression. The associated covariate vector has been referred to as a ‘J-factor’, which if fitted as a fixed effect can improve the accuracy and dispersion bias of sire genomic estimated breeding values (GEBV). To date, this has only been performed on populations with a single breed. Here, we investigated whether there was any benefit in fitting a separate J-factor for each breed in a three-way crossbred population, and in using pedigree-based expected or genome-based estimated breed fractions to define the J-factors.

Results
For body weight at 7 days, dispersion bias decreased when fitting multiple J-factors, but only with a low proportion of genotyped individuals with selective genotyping. On average, the mean regression coefficients of validation records on those of GEBV increased with one J-factor compared to none, and further increased with multiple J-factors. However, for body weight at 35 days this was not observed. The accuracy of GEBV remained unchanged regardless of the J-factor method used. Differences between the J-factor methods were limited with correlations approaching 1 for the estimated covariate vector, the estimated coefficients of the regression on the J-factors, and the GEBV.

Conclusions
Based on our results and in the particular design analysed here, i.e. all the animals with phenotype are of the same type of crossbreds, fitting a single J-factor should be sufficient, to reduce dispersion bias. Fitting multiple J-factors may reduce dispersion bias further but this depends on the trait and genotyping rate. For the crossbred population analysed, fitting multiple J-factors has no adverse consequences and if this is done, it does not matter if the breed fractions used are based on the pedigree-expectation or the genomic estimates. Finally, when GEBV are estimated from crossbred data, any observed bias can potentially be reduced by including a straightforward regression on actual breed proportions.