bluuebunny/crossref_metadata_2025_split huggingface dataset:
The NE/AAT/CBG axis regulates adipose tissue glucocorticoid exposure
Authors: Boyle Luke D. , Miguelez-Crespo Allende , Paul Mhairi , Villalobos Elisa , Toews Julia N. C. , Ivatt Lisa , Nagy Boglarka , Magennis Marisa , Homer Natalie Z. M. , Andrew Ruth , Viau Victor , Hammond Geoffrey L. , Stimson Roland H. , Walker Brian R. , Nixon Mark 
Published: January 2025

AbstractCorticosteroid binding globulin (CBG; SERPINA6) binds >85% of circulating glucocorticoids but its influence on their metabolic actions is unproven. Targeted proteolytic cleavage of CBG by neutrophil elastase (NE; ELANE) significantly reduces CBG binding affinity, potentially increasing ‘free’ glucocorticoid levels at sites of inflammation. NE is inhibited by alpha-1-antitrypsin (AAT; SERPINA1). Using complementary approaches in mice and humans to manipulate NE or AAT, we show high-fat diet (HFD) increases the NE:AAT ratio specifically in murine visceral adipose tissue, an effect only observed in males. Notably, HFD-fed male mice lacking NE have reduced glucocorticoid levels and action specifically in visceral adipose tissue, with improved glucose tolerance and insulin sensitivity, independent of systemic changes in free glucocorticoids. The protective effect of NE deficiency is lost when the adrenals are removed. Moreover, human asymptomatic heterozygous carriers of deleterious mutations in SERPINA1 resulting in lower AAT levels have increased adipose tissue glucocorticoid levels and action. However, in contrast to mice, humans present with systemic increases in free circulating glucocorticoid levels, an effect independent of HPA axis activation. These findings show that NE and AAT regulate local tissue glucocorticoid bioavailability in vivo, providing crucial evidence of a mechanism linking inflammation and metabolism.
Towards data-driven electricity management: multi-region uniform data and knowledge graph
Authors: Hanžel Vid , Bertalanič Blaž , Fortuna Carolina 
Published: January 2025

AbstractDue to growing population and technological advances, global electricity consumption is increasing. Although CO2 emissions are projected to plateau or slightly decrease by 2025 due to the adoption of clean energy sources, they are still not decreasing enough to mitigate climate change. The residential sector makes up 25% of global electricity consumption and has potential to improve efficiency and reduce CO2 footprint without sacrificing comfort. However, a lack of uniform consumption data at the household level spanning multiple regions hinders large-scale studies and robust multi-region model development. This paper introduces a multi-region dataset compiled from publicly available sources and presented in a uniform format. This data enables machine learning tasks such as disaggregation, demand forecasting, appliance ON/OFF classification, etc. Furthermore, we develop an RDF knowledge graph that characterizes the electricity consumption of the households and contextualizes it with household-related properties enabling semantic queries and interoperability with other open knowledge bases like Wikidata and DBpedia. This structured data can be utilized to inform various stakeholders towards data-driven policy and business development.
Prediction of the toughness of date palm fruit
Authors: Hassan Ahmed M. , Ibrahim Mohamed M. , Ghonimy Mohamed , Fathy Maher 
Published: January 2025

AbstractThis study aimed to predict the toughness of date palm fruit (Barhi, Saqie, and Khodry varieties) at different ripening stages (Khalal, Rutab, and Tamar) using Hertz Theory by evaluating the physical and mechanical characteristics of the fruits. Physical measurements revealed that high moisture content in the Khalal stage led to larger dimensions and mass across all varieties, with Barhi dates showing a moisture content of 63.31%, which decreased to 32.60% at Rutab as the fruit dehydrated. This moisture reduction also impacted other characteristics, such as bulk density, volume, and flesh thickness, resulting in a concentrated, denser structure in the Tamar stage. Mechanical analysis demonstrated significant softening in all varieties as they transitioned from Khalal to Rutab stages, with Barhi dates’ modulus of elasticity dropping from 548.83 to 16.72 kPa. Similarly, bioyield and rupture stress decreased, highlighting the influence of moisture loss on textural characteristics. Saqie and Khodry varieties followed similar trends, with initial softening from Khalal to Rutab, and a slight firmness increase at Tamar due to dehydration. The force-deformation curves and toughness measurements confirmed these findings, showing significant reductions in toughness from Khalal to Rutab, with a slight increase in Tamar. These results underscore the pronounced textural and structural changes as dates ripen, influencing their suitability for different applications. Also, the measured toughness (τm) closely aligned with predicted toughness (τp), with significant textural differences observed across varieties and ripening stages. For all varieties, the τp is approximately 0.00–9.24% lower than the τm. Finally, the derived equation can be used with enough confidence to theoretically predict the toughness of date palm fruit.
Experimental and numerical analysis of pGFRP and wood cross-arm in latticed tower: a comprehensive study of mechanical deformation and flexural creep
Authors: Latif Amir Abd , Ishak Mohamad Ridzwan , Razman Muhammad Rizal , Yidris Noorfaizal , Mohd Zuhri Mohamed Yusoff , Rizal Muhammad Asyraf Muhammad , Ramli Zuliskandar 
Published: January 2025

AbstractThe adoption of pultruded glass fibre-reinforced polymer (pGFRP) composites as a substitute for traditional wooden cross-arms in high transmission towers represents a relatively novel approach. These materials were selected for their high strength-to-weight ratio and lightweight properties. Despite various studies focusing on structures improvement, there still have a significant gap in understanding the deformation characteristics of full-scale cross-arms under actual operational loads. Existing study often concentrate on small coupon scale and laboratory condition, leaving a gap in understanding how the cross-arm behavior in full-scale acting on actual weather condition. This study aims to investigate the load-deflection and long-term creep behavior of a pGFRP cross-arm installed in a 132 kV transmission tower. The pGFRP cross-arm was load-tested on a customized rig in an open environment. Using the cantilever beam concept, deflection was analyzed and compared to wood cross-arms. Finite element analysis validated results, and long-term deformation under high-stress loads was assessed. The pGFRP cross-arms showed lower deflection at working loads compared to Balau wood, due to the latter’s higher elastic modulus and flexibility specifically at Point Y3, the critical issues necessitated reinforcement strategies. pGFRP cross-arms withstood higher bending stress, showing 32% less deflection under normal conditions and 15% less under broken wire conditions than Balau wood. Additionally, the creep strength of wood was 34% lower than that of pGFRP cross-arms. Besides that, the pGFRP cross-arm have highest elastic modulus than Balau-wood, shows that the composite cross-arm have better structural strength, resisting deformation and higher flexibility materials. Finite element analysis (FEA) confirmed these results with the relative error between them less than 1%. Consequently, the investigation into pGFRP cross-arm deformation behavior in this paper serves as a foundational framework for future research endeavors specifically for high transmission tower and other structural application.
Systematic review and meta-analysis of pain management after tonsillectomy
Authors: Geißler Katharina , Scham Daniel , Meißner Winfried , Schlattmann Peter , Guntinas-Lichius Orlando 
Published: January 2025

AbstractTonsillectomy is one of the most common operations. Tonsillectomy is also one of the most painful surgical procedures. However, there is still no satisfactory standard for postoperative pain management. Four databases (Cochrane Library, Ovid Technologies, PubMed, Web of Science) were searched for the period from 1908 to 2019. The systematic literature review followed the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines. Data were pooled using random-effects and fixed-effects models. Randomized controlled trials, reviews and meta-analyses were included. Primary outcomes were quantitative pain intensity in the first 24 h after tonsillectomy and on days 1, 3, and 7 postoperatively. The search yielded 1594 publications, of which 111 publications with 7566 patients, both children and adults, could be included. Intraoperative medication with intravenous dexamethasone significantly reduced pain (mean difference [MD] -0.42; 95% confidence interval [CI]: -0.61- -0.24). Among the local anesthetics, only the preoperative injection of levobupivacaine into the tonsillar compartment was able to provide sufficient pain reduction up to three days after tonsillectomy (MD: -1.92; 95% CI: -2.73 - -1.11). Preoperative or intraoperative administration of non-steroidal anti-inflammatory drugs (NSAIDs) significantly reduced pain (MD: -0.75; 95% CI: -0.87- -0.63). Steroids and NSAIDs are an important part of pain management after tonsillectomy.
Asymptomatic infection and antibody prevalence to co-occurring avian influenza viruses vary substantially between sympatric seabird species following H5N1 outbreaks
Authors: Greco Fiona , Ravenswater Hannah M. , Ruiz-Raya Francisco , D’Avino Chiara , Newell Mark A. , Hewitt Josie , Taylor Erin , Benninghaus Ella , Daunt Francis , Goodman Gidona , Steel David , Park Jenny , Philip Emma , Thomas Saumya S. , Slomka Marek J. , Falchieri Marco , Reid Scott M. , James Joe , Banyard Ashley C. , Burthe Sarah J. , Cunningham Emma J. A. 
Published: January 2025

AbstractEmerging infectious diseases are of major concern to animal and human health. Recent emergence of high pathogenicity avian influenza virus (HPAIV) (H5N1 clade 2.3.4.4b) led to substantial global mortality across a range of host species. Co-occurring species showed marked differences in mortality, generating an urgent need for better epidemiological understanding within affected populations. We therefore tested for antibodies, indicative of previous exposure and recovery, and for active viral infection in apparently healthy individuals (n = 350) across five co-occurring seabird species on the Isle of May, Scotland, during 2023, following H5N1 HPAIV associated mortality in the preceding summer. Antibody prevalence to AIV subtypes varied substantially between species, ranging from 1.1% in European shags (Gulosus aristotelis) (to H5) to 78.7% in black-legged kittiwakes (Rissa tridactyla) (to H16 or both H13 and H16), and between 31 and 41% for three auk species (H5, H16 or both). At least 20.4% of auks had antibodies to an as yet unidentified subtype, suggesting further subtypes circulating in the population. We found low levels of active, but asymptomatic, AIV infection in individuals (1.6–4.5%), but excluded this as H5N1. Our results emphasise the importance of testing healthy individuals to understand the prevalence of co-circulating AIV subtypes in wild populations, and the potential for future reassortment events which could alter virus behaviour and impact.
Associations of serum and tissue TIMP1 with host response and survival in colorectal cancer
Authors: Kehusmaa Akseli , Tuomisto Anne , Sirniö Päivi , Karjalainen Henna , Kastinen Meeri , Tapiainen Vilja V. , Äijälä Ville K. , Tervahartiala Taina , Sorsa Timo , Rintala Jukka , Meriläinen Sanna , Saarnio Juha , Rautio Tero , Mäkinen Markus J. , Väyrynen Juha P. 
Published: January 2025

AbstractTissue inhibitor of matrix metalloproteinase 1 (TIMP1) is a multifaceted, cytokine-like bioactive molecule whose levels are elevated in a wide range of inflammatory diseases and are associated with prognosis. Additionally, TIMP1 may play a role in driving systemic inflammation. TIMP1 immunohistochemistry and TIMP1 serum concentrations were analyzed in a cohort of 776 colorectal cancer patients. TIMP1 histoscore by cell type (tumor cell, other) was quantified using digital image analysis. Serum TIMP1 levels were evaluated for correlations with tumor TIMP1 expression, and their associations with tumor characteristics, inflammation, and prognosis were investigated. High serum TIMP1 concentrations associated with shorter overall survival (multivariable HR 1.85, 95% CI 1.30–2.65). Serum TIMP1 levels positively correlated with markers of systemic inflammation and tumor necrosis percentage but not with TIMP1 expression in tumor tissue. High TIMP1 intensity in tumor stroma associated with longer cancer-specific and overall survival in univariable analysis but not in multivariable models. T cell densities in tumor tissue positively correlated with tumor stromal TIMP1 expression and negatively with tumor epithelial TIMP1 expression. Serum TIMP1 levels show promise as a prognostic marker for colorectal cancer and correlate with systemic inflammatory markers, but do not correlate with TIMP1 expression in tumor tissue.
Transcriptome-wide association study identifies genes associated with bladder cancer risk
Authors: Li Siting , Gui Jiang , Karagas Margaret R. , Passarelli Michael N. 
Published: January 2025

AbstractGenome-wide association studies (GWAS) have detected several susceptibility variants for urinary bladder cancer, but how gene regulation affects disease development remains unclear. To extend GWAS findings, we conducted a transcriptome-wide association study (TWAS) using PrediXcan to predict gene expression levels in whole blood using genome-wide genotype data for 6180 bladder cancer cases and 5699 controls included in the database of Genotypes and Phenotypes (dbGaP). Logistic regression was used to estimate adjusted gene-level odds ratios (OR) per 1-standard deviation higher expression with 95% confidence intervals (CI) for bladder cancer risk. We further assessed associations for individual single-nucleotide polymorphisms (SNPs) used to predict expression levels and proximal loci for genes identified in gene-level analyses with false-discovery rate (FDR) correction. TWAS identified four genes for which expression levels were associated with bladder cancer risk: SLC39A3 (OR = 0.91, CI = 0.87–0.95, FDR = 0.015), ZNF737 (OR = 0.91, CI = 0.88–0.95, FDR = 0.016), FAM53A (OR = 1.09, CI = 1.05–1.14, FDR = 0.022), and PPP1R2 (OR = 1.09, CI = 1.05–1.13, FDR = 0.049). Findings from this TWAS enhance our understanding of how genetically-regulated gene expression affects bladder cancer development and point to potential prevention and treatment targets.
Exploratory disproportionality analysis of potentially drug-induced eosinophilic pneumonia using United States Food and Drug Administration adverse event reporting system
Authors: Kamath Ashwin 
Published: January 2025

AbstractDrug-induced eosinophilic pneumonia (EP) is an uncommon adverse drug reaction. Many drugs have been reported to cause EP, the evidence mainly being in the form of case reports/case series. This study aims to conduct an exploratory analysis of the United States Food and Drug Administration adverse event reporting system (FAERS) database to identify previously unknown drugs that can cause EP and supplement the available evidence for known culprit drugs. A retrospective case–noncase study was conducted using individual case safety reports (ICSRs) reported to the US FAERS from the first quarter of 2004 to the second quarter of 2024. Cases of potentially drug-induced EP were identified using OpenVigil application by conducting a narrow and broad scope search using the Medical Dictionary of Regulatory Activities preferred terms. A base list of drugs described in select literature to have caused EP was used to categorize known and unknown drugs. A disproportionality analysis was performed, with a reporting odds ratio > 2, lower end of the 95% confidence interval > 1, and a minimum of 3 reported cases considered a signal of disproportionate reporting (SDR). During the study period, 8,702,548 individual case safety reports (ICSRs) were submitted to the FAERS. Of these, 855 ICSRs using the narrow scope search and 1411 ICSRs using the broad scope search reported EP. The three most commonly reported drugs with an SDR for EP using the narrow scope search were daptomycin, naltrexone, and prednisone. The most common indications for the use of the drugs were infections, immunological conditions, asthma, and central nervous system disorders. In total, there were 45 drugs with an SDR but no supporting literature evidence available. The number of drugs implicated in causing EP has increased over the years. Several antimicrobial agents, followed by drugs affecting the central nervous system and anticancer drugs, including monoclonal antibodies, can produce EP. The list of suspected drugs identified in this study, especially those with SDR and literature evidence, should be strongly considered as a possible cause in patients presenting with pneumonia not explained otherwise.
The importance of clinical experience in AI-assisted corneal diagnosis: verification using intentional AI misleading
Authors: Maehara Hiroki , Ueno Yuta , Yamaguchi Takefumi , Kitaguchi Yoshiyuki , Miyazaki Dai , Nejima Ryohei , Inomata Takenori , Kato Naoko , Chikama Tai-ichiro , Ominato Jun , Yunoki Tatsuya , Tsubota Kinya , Oda Masahiro , Suzutani Manabu , Sekiryu Tetsuju , Oshika Tetsuro 
Published: January 2025

AbstractWe developed an AI system capable of automatically classifying anterior eye images as either normal or indicative of corneal diseases. This study aims to investigate the influence of AI’s misleading guidance on ophthalmologists’ responses. This cross-sectional study included 30 cases each of infectious and immunological keratitis. Responses regarding the presence of infection were collected from 7 corneal specialists and 16 non-corneal-specialist ophthalmologists, first based on the images alone and then after presenting the AI’s classification results. The AI’s diagnoses were deliberately altered to present a correct classification in 70% of the cases and incorrect in 30%. The overall accuracy of the ophthalmologists did not significantly change after AI assistance was introduced [75.2 ± 8.1%, 75.9 ± 7.2%, respectively (P = 0.59)]. In cases where the AI presented incorrect diagnoses, the accuracy of corneal specialists before and after AI assistance was showing no significant change [60.3 ± 35.2% and 53.2 ± 30.9%, respectively (P = 0.11)]. In contrast, the accuracy for non-corneal specialists dropped significantly from 54.5 ± 27.8% to 31.6 ± 29.3% (P < 0.001), especially in cases where the AI presented incorrect options. Less experienced ophthalmologists were misled due to incorrect AI guidance, but corneal specialists were not. Even with the introduction of AI diagnostic support systems, the importance of ophthalmologist’s experience remains crucial.
Genetic and epigenetic characterization of sarcoma stem cells across subtypes identifies EZH2 as a therapeutic target
Authors: O’Donnell Edmond , Muñoz Maria , Davis Ryan , Bergonio Jessica , Randall R. Lor , Tepper Clifford , Carr-Ascher Janai 
Published: January 2025

AbstractHigh-grade soft tissue sarcomas (STS) are a heterogeneous and aggressive set of cancers. Failure to respond anthracycline chemotherapy, standard first-line treatment, is associated with poor outcomes. We investigated the contribution of STS cancer stem cells (STS-CSCs) to doxorubicin resistance. We identified a positive correlation between CSC abundance and doxorubicin IC50. Utilizing patient-derived samples from five sarcoma subtypes we investigated if a common genetic signature across STS-CSCs could be targeted. We identified Enhancer of Zeste homolog 2 (EZH2), a member of the polycomb repressive complex 2 (PRC2) responsible for H3K27 methylation as being enriched in CSCs. EZH2 activity and a shared epigenetic profile was observed across subtypes and targeting of EZH2 ablated the STS-CSC population. Treatment of doxorubicin-resistant cell lines with tazemetostat resulted in a decrease in the STS-CSC population. These data confirm the presence of shared genetic programs across distinct subtypes of CSC-STS that can be therapeutically targeted.
Standardized patient profile review using large language models for case adjudication in observational research
Authors: Schuemie Martijn J. , Ostropolets Anna , Zhuk Aleh , Korsik Uladzislau , Seo Seung In , Suchard Marc A. , Hripcsak George , Ryan Patrick B. 
Published: January 2025

AbstractUsing administrative claims and electronic health records for observational studies is common but challenging due to data limitations. Researchers rely on phenotype algorithms, requiring labor-intensive chart reviews for validation. This study investigates whether case adjudication using the previously introduced Knowledge-Enhanced Electronic Profile Review (KEEPER) system with large language models (LLMs) is feasible and could serve as a viable alternative to manual chart review. The task involves adjudicating cases identified by a phenotype algorithm, with KEEPER extracting predefined findings such as symptoms, comorbidities, and treatments from structured data. LLMs then evaluate KEEPER outputs to determine whether a patient truly qualifies as a case. We tested four LLMs including GPT-4, hosted locally to ensure privacy. Using zero-shot prompting and iterative prompt optimization, we found LLM performance, across ten diseases, varied by prompt and model, with sensitivities from 78 to 98% and specificities from 48 to 98%, indicating promise for automating phenotype evaluation.
Cementless Short Stems in Total Hip Arthroplasty: Chances and Limits
Authors: Afghanyar Yama , Kutzner Karl Philipp , Pfeil Joachim , Drees Philipp , Rehbein Philipp , Dargel Jens 
Published: January 2025

AbstractIn recent decades, a large number of cementless short stems have been introduced to the market. The concept aims at saving soft tissue using minimally invasive surgery and at the same time preserving as much bone as possible. In particular, the latest generation of short stems, which are implanted using a calcar-guided round-the-corner technique, are attracting increasing attention. An individualised resection level allows individual stem alignment and thus an ideal reconstruction of the hip anatomy. The early clinical results of short-stem total hip arthroplasty (THA) are promising and have led to an expansion of the indications and limitations for the use of short stems. In particular, the individual positioning in valgus or varus and the resulting individual metaphyseal or metadiaphyseal anchorage offers various possibilities to reconstruct even abnormal joint morphologies. Consequently, short stems are increasingly used in patients with complex anatomical variations or in cases of osteonecrosis of the femoral head. In some various cases, they can also be used in revision or conversion arthroplasty. In some patients, short stems can also be used after femoral neck fracture. Currently, scientific data on those areas of indication of short-stem THA is scarce.