bluuebunny/crossref_metadata_2025_split huggingface dataset:
Consumer acceptability of no added sugar pound cakes under blind and informed testing conditions
Authors: Williams Reed , Moazzem Md Shakir , Dees Jacob , Hayden Michelle , Traynor Mark , Cho Sungeun 
Published: September 2023

AbstractStevia is an emerging natural high‐intensity sweetener. There are negative perceptions of zero‐calorie sweeteners, but studies that provide knowledge of these sweeteners improve their perception. This study evaluated consumer acceptability of a zero‐sugar bakery product under blind and informed conditions (n = 96) along with physicochemical analysis of the products. Rebaudioside A (Reb A) and the new types of stevia (Rebs D and M) with sugar as a control were used to formulate pound cakes. Panelists evaluated the overall hedonic impressions (aroma, texture, flavor, and aftertaste) and intensity (sweetness and bitterness) of the cakes under blind and informed conditions with an enforced 2‐week break between evaluations. During the informed session, a document was provided prior to evaluating samples that included stevia's health benefits and the nutritional facts panels for the cakes. The cakes underwent volatile profile (electronic nose [e‐nose]) and water activity (aw) analysis. Overall, stevia cakes showed an increase in flavor and texture liking during the informed session when compared to the blind session, but only Reb A showed a significant difference (p < 0.05). The increase in liking scores indicated that information positively affected the consumer's perception of the stevia‐sweetened cakes attributes. The e‐nose confirmed differences in aroma. There was a significant difference in aw of the samples Rebs A, D, M versus sucrose (p < 0.05). No significant differences were observed among the Rebs (p > 0.05). This study illustrates that stevia, despite non‐browning or fermenting, can be used in a practical baking application, and product‐related information impacts consumer acceptability.Practical ApplicationThis study demonstrates that product‐related information may have an impact on the consumer acceptability of the product. Through potential labeling improvements, overall consumer perception and acceptability of zero‐sugar added or low‐sugar products could be improved. This study also illustrates that stevia, despite being a non‐browning or fermenting sugar alternative, can be used in a practical baking application.
Clinical characteristics of patients with KRAS mutation detected by liquid biopsy
Authors: Amino Yoshiaki , Low Siew‐Kee , Ninomiya Hironori , Kiritani Ayu , Miyadera Keiki , Kakuto Sho , Akita Takahiro , Tsugitomi Ryosuke , Ariyasu Ryo , Uchibori Ken , Kitazono Satoru , Yanagitani Noriko , Nishio Makoto 
Published: September 2023

AbstractBackgroundKRAS mutation positive lung cancer is known to be clinically characterized by older age, males, and smokers. It is reported to be more common in mucinous adenocarcinoma, but all reports are based on analysis of tissue samples. Recently, blood samples have become available for analysis, suggesting a low detection rate of circulating tumor DNA in histological types, especially mucinous adenocarcinoma. In this study, we investigated the clinical characteristics of KRAS mutation‐positive cases in the analysis of blood specimens, as these remain unclear.MethodsThe clinical background of patients with KRAS mutation among those who underwent next‐generation sequencing (NGS) analysis using blood samples was evaluated.ResultsNGS analysis was performed on 214 blood samples. KRAS mutations were detected in blood samples in 33 cases (15.4%), of which 31 cases (14.5%) had a histological pathology diagnosis. Mucinous adenocarcinoma accounted for 28.6% of cases with positive blood and tissue specimens, 10.0% of cases with positive blood specimens only, and 57.1% of cases with positive tissue specimens only. Mucinous adenocarcinoma tended to be less common in cases with positive blood specimens. In KRAS‐positive patients with lung metastasis only, only one nonmucinous adenocarcinoma had a positive blood sample, and the others all had mucinous adenocarcinomas with positive tissue samples only.ConclusionThe results showed that the detection rate of KRAS‐positive lung cancers detected by blood and tissue samples differs, and that the detection rate of blood samples may be poor, especially in the case of mucinous adenocarcinoma with lung metastases only.
Identification and pre‐clinical investigation of 3‐O‐cyclohexanecarbonyl‐11‐keto‐β‐boswellic acid as a drug for external use to treat psoriasis
Authors: Lou Fangzhou , Xu Zhenyao , Bai Jing , Zhao Xing'e , Cui Linlin , Li Qun , Wang Honglin 
Published: September 2023

AbstractBackground and PurposePsoriasis vulgaris is a refractory skin inflammatory disorder with 80% of the cases belonging to the mild‐to‐moderate type, which can be controlled by topical treatment. Nevertheless, the drugs for external use have not been upgraded for decades. We modified acetyl‐11‐keto‐beta‐boswellic acid (ABKA), a natural compound shown to treat psoriasis animal models, to improve efficacy and solubility for topical use.Experimental ApproachEleven compounds were synthesized using AKBA as a lead compound, and their effects on Th17 cell differentiation were screened. 3‐O‐cyclohexanecarbonyl‐11‐keto‐β‐boswellic acid (CKBA) potently inhibited Th17 cell differentiation. Its efficacy in a mouse model of psoriasis was assessed along with its pharmacology and safety profile when topically or systemically delivered to several animal species.Key ResultsCKBA inhibited mouse and human Th17 cell differentiation with an IC50 of 3.28 and 3.61 μM, respectively, and directly targeted acetyl‐CoA carboxylase 1 (ACC1). Safety evaluation and toxicity tests suggested that systemically delivered high‐dose CKBA for 14 days had no dose‐associated adverse effects on the CNS, haematopoietic, cardiovascular, respiratory and digestive systems of cynomolgus monkeys. CKBA ointment permeated the skin and did not irritate or sensitize intact skin. CKBA ointment mediated dose‐dependent suppression of imiquimod‐induced psoriasis‐like skin inflammation with slow absorption and limited bioavailability (<10% in rats and <1% in minipigs).Conclusions and ImplicationsCKBA is safe when topically or systemically delivered to animals. The beneficial effects of CKBA ointment in a mouse model of psoriasis indicate that this is a promising drug candidate for further development as a treatment for psoriasis.
Oral function in completely edentulous patients rehabilitated with implant‐supported dental prostheses: A systematic review and meta‐analysis
Authors: Srinivasan Murali , Kamnoedboon Porawit , Angst Lea , Müller Frauke 
Published: September 2023

AbstractObjectivesThis review evaluated the effects of rehabilitation with implant‐supported prostheses on the oral function of completely edentulous adults.Materials and MethodsSystematic literature searches were performed to identify records reporting on oral function in completely edentulous adults rehabilitated with implant‐supported prostheses. Meta‐analyses were performed on various outcomes including bite force, masticatory performance, muscle activity, mandibular movement/chewing pattern, and salivary flow.Results5507 records were identified. Thirty studies qualified for data extraction and analysis. The kappa (𝛋) for the search and identification strategy ranged between 0.50 and 1.00. Meta‐analysis was performed grouping the studies by outcomes and split by time points of 6–12 months, 12–36 months, and >36 months after implant therapy. The meta‐analyses revealed a significant improvement in oral function of completely edentulous adults after rehabilitation with implant‐supported prostheses at 6–12 months (Z = −4.895, p < .001; 95% CI: −0.703, −0.301; τ2 = .609; Q = 114.953, df = 17, p < .001; I2 = 85.2%), at 12–36 months (Z = −4.886, p < .001; 95% CI: −0.580, −0.248; τ2 = .908; Q = 280.611, df = 35, p < .001; I2 = 87.5%) and at more than 36 months (Z = −9.108, p < .001; 95% CI: −1.472, −0.951; τ2 = .019; Q = 7.918, df = 7, p = .340; I2 = 11.6%). The included studies demonstrated a low to moderate risk of bias.ConclusionsThis systematic review concluded that the oral function of completely edentate adults significantly improved with implant‐supported/retained prostheses, even when only one jaw received implant therapy. Therefore, implant therapy should be promoted for edentulous adults to alleviate the shortcomings of conventional complete removable dental prostheses.
Clinical performance of single implant prostheses restored using titanium base abutments: A systematic review and meta‐analysis
Authors: Chantler Jennifer G. M. , Evans Christopher D. J. , Zitzmann Nicola U. , Derksen Wiebe 
Published: September 2023

AbstractPurposeThe aim of this review was to evaluate the survival rates of restorations utilizing titanium base abutments (TBA) for restoring single‐unit implant prostheses.Materials and MethodsThis review was conducted following the preferred reporting items for systematic reviews and meta‐analyses (PRISMA) guidelines. The focus question was: In patients who require the restoration of a single dental implant utilizing a titanium base abutment, what are the determining factors and outcomes relating to implant prosthesis prognosis and survival? A comprehensive search of databases (PubMed, EMBASE, and Cochrane Library) was conducted on 16 April 2023 and updated on 5 May 2023. Randomized clinical trials (RCT), retrospective studies and prospective studies, reporting on the use of TBA for single implant prostheses, were reviewed. A Cochrane collaboration risk of bias assessment analysis was performed for randomized clinical studies, and the Newcastle–Ottawa Scale tool was applied for non‐randomized studies. A meta‐analysis was performed on clinical trials reporting on survival rates of both TBA and other abutments. Other clinical studies, reporting on TBA only, were included for descriptive statistics.ResultsThe search provided 1159 titles after duplicates were removed. Six RCTs were included to perform a meta‐analysis and compare the survival of the TBA to other abutments [OR 0.74; 95% CI: 0.21–2.63, heterogeneity; I2 0%; p = .99]. Twenty‐three prospective and retrospective studies fulfilled the criteria and were included in the meta‐analysis after 12 months of function. A total of 857 single implant‐supported prostheses fabricated with a TBA were included. TBA abutments have an estimate 98.6% survival rate after 1 year in function (95% CI: 97.9%–99.4%). The mean follow‐up period was 31.2 ± 16.9 months.ConclusionsSingle implant prosthesis restored with titanium base abutments showed favourable short‐term survival rates.
Effect of agents affecting bone homeostasis on short‐ and long‐term implant failure
Authors: Jung Junho , Ryu Jae‐In , Shim Gyu‐Jo , Kwon Yong‐Dae 
Published: September 2023

AbstractObjectivesTo review the current evidence on the relationship between agents that affect bone homeostasis and dental implant failures.Materials and MethodsElectronic searches for bisphosphonates, denosumab, methotrexate, corticosteroids, romosozumab, sunitinib, and bevacizumab were performed using PubMed, MEDLINE (OVID), EMBASE (OVID), Cochrane Central Register of Controlled Trials (Cochrane Library), Cochrane Oral Health Group Trials Register (Cochrane Library) and Web of Science (Thomson Reuters). Manual searches were also conducted to complement the digital searches for recent issues.ResultsPrevious publications suggested that bisphosphonates do not compromise the survival of dental implants. However, one study documented an increased risk of implant failure in patients who had received high‐dose of intravenous bisphosphonate therapy after implant rehabilitation. There has been an issue of MRONJ around implants in patients who have successfully received implant therapy before and after antiresorptive therapy, leading to late implant failure. Despite evidence on the detrimental effects of denosumab, methotrexate and corticosteroids on bone metabolism, their role in implant survival is not conclusive.ConclusionsAt present, there is insufficient evidence to establish a potential connection between agents that affects bone homeostasis and implant failure. However, some studies have reported negative results for implant therapy. In addition, implant‐related sequestration in patients who received anti‐resorptive therapy, despite of successful osseointegration, is also noticeable. Although limited studies are available at present, clinicians should still carefully consider the potential hazards and take appropriate precautions to minimize the risks associated with the medications and implant therapy.
Delayed‐type allergic reaction to furazolidone: A case report and review of the literature
Authors: Xu Feng , Zhang Hongting , Xiao Hao , Meng Juan 
Published: September 2023

AbstractBackgroundFurazolidone is a nitrofuran antimicrobial agent used in the treatment of bacterial and protozoal infections. Hypersensitivity to furazolidone is rarely reported and only eight cases have been documented in English since 1967.ObjectivesTo report a 24‐year‐old man who developed exanthematous drug eruptions in general and swelling sensation of the hands after first dose of oral administration of medicines for Helicobacter pylori infection 7 h later, who was finally confirmed with delayed‐type IV allergic reaction to furazolidone by provocation tests. And to review the existing literature.MethodsThorough clinical examination, prick, intradermal, and patch tests, drug provocation tests were performed in the patient.ResultsSkin tests of all used drugs were negative. Drug provocation tests to furazolidone resulted to be positive.ConclusionsClinicians should be aware that furazolidone may induce delayed‐type allergic reactions; diagnostic approaches should be taken to identify the responsible drug when multiple medications were used concurrently.
Pyruvate kinase M2 regulates kidney fibrosis through pericyte glycolysis during the progression from acute kidney injury to chronic kidney disease
Authors: Chen Yulan , Bai Xueyuan , Chen Jianwen , Huang Mengjie , Hong Quan , Ouyang Qing , Sun Xuefeng , Zhang Yan , Liu Jiaona , Wang Xu , Wu Lingling , Chen Xiangmei 
Published: September 2023

AbstractWe aimed to investigate the role of renal pericyte pyruvate kinase M2 (PKM2) in the progression of acute kidney injury (AKI) to chronic kidney disease (CKD). The role of PKM2 in renal pericyte‐myofibroblast transdifferentiation was investigated in an AKI‐CKD mouse model. Platelet growth factor receptor beta (PDGFRβ)‐iCreERT2; tdTomato mice were used for renal pericyte tracing. Western blotting and immunofluorescence staining were used to examine protein expression. An 5‐ethynyl‐2′‐deoxyuridine assay was used to measure renal pericyte proliferation. A scratch cell migration assay was used to analyse cell migration. Seahorse experiments were used to examine glycolytic rates. Enzyme‐linked immunoassay was used to measure pyruvate kinase enzymatic activity and lactate concentrations. The PKM2 nuclear translocation inhibitors Shikonin and TEPP‐46 were used to alter pericyte transdifferentiation. In AKI‐CKD, renal pericytes proliferated and transdifferentiated into myofibroblasts and PKM2 is highly expressed in renal pericytes. Shikonin and TEPP‐46 inhibited pericyte proliferation, migration, and pericyte‐myofibroblast transdifferentiation by reducing nuclear PKM2 entry. In the nucleus, PKM2 promoted downstream lactate dehydrogenase A (LDHA) and glucose transporter 1 (GLUT1) transcription, which are critical for glycolysis. Therefore, PKM2 regulates pericyte glycolytic and lactate production, which regulates renal pericyte‐myofibroblast transdifferentiation. PKM2‐regulated renal pericyte‐myofibroblast transdifferentiation by regulating downstream LDHA and GLUT1 transcription and lactate production. Reducing nuclear PKM2 import can reduce renal pericytes‐myofibroblasts transdifferentiation, providing new ideas for AKI‐CKD treatment.
National resident survey in dermatopathology: The role of slide scanners in resident learning
Authors: Rinck Danielle , Dittmer Martin , Tinker Daniel , Smith Kristin , Heinecke Gillian 
Published: September 2023

AbstractBackgroundDermatology residents gain exposure to dermatopathology through a variety of educational modalities. While virtual pathology applications have risen dramatically, resident utilization of digital libraries, slide scanner availability, and comfort with virtual slides are not well‐known. This study aims to assess the current landscape of educational resources used by dermatology residents.MethodsA 17‐question survey was sent to dermatology residents through a national email database. The survey was a self‐assessment of their experience in dermatopathology education and the use of departmental slide scanners.ResultsThe use of digital dermatopathology is high among trainees, despite only half of respondents reporting slide scanner access. Residents report using virtual images more often in non‐clinical dermatopathology didactics and independent studies compared to clinical dermatopathology rotations. Public slide set use was common, while professional society and departmental slide sets may be underutilized. Over half of respondents report being extremely or very comfortable navigating interactive scanned slides.ConclusionsSurvey data suggests digital slides are currently predominantly used in non‐clinical dermatopathology rotations and independent studies. Incorporation of slide scanners into departments may benefit resident education through the development of high‐quality, curated departmental slide sets.
Using automated content analysis to monitor global online trade in endemic reptile species
Authors: Rinne Jooel , Kulkarni Ritwik , Soriano‐Redondo Andrea , Correia Ricardo , Di Minin Enrico 
Published: September 2023

AbstractAimOnline reptile trade poses new challenges to species conservation and requires automated monitoring. Range‐restricted and endemic reptile species are especially vulnerable to wildlife trade and unsustainable exploitation. In this study, we investigated the magnitude and geographic distribution of online trade of 96 endemic and range‐restricted reptile species from the Lesser Antilles.LocationGlobal.MethodsWe developed methods for automated collection, filtering and processing of wildlife trade content for the targeted species from publicly accessible online platforms.ResultsWe identified 599 relevant advertisements originating from 231 different advertisers and 41 websites focusing on 43 species. Species advertised included threatened species according to the International Union for the Conservation of Nature (IUCN) Red List and species listed in the Convention on International Trade in Endangered Species of Wild Fauna and Flora (CITES) Appendices. Among threatened species, five are Critically Endangered, three are Endangered and two are Vulnerable according to the IUCN Red List. Moreover, three of the six most advertised species were classified as Near Threatened. Germany was the country with the highest number of advertisements (N = 124), followed by the United States (N = 55), the Netherlands (N = 15) and United Kingdom (N = 15). Based on data from sale advertisements that included price and currency data, prices ranged from one to over a thousand Euros.Main ConclusionsWe present a framework for automated analysis of online trade in reptiles that can be extended to other taxonomic groups. Our results highlight countries, such as Germany and the United States, where enhanced monitoring actions would be important to assess the origin (i.e. captive bred or wild sourced individuals) and the legality of the trade. Immediate conservation actions, such as population monitoring, are also needed to ensure wildlife trade is not threatening the persistence of endemic reptile populations in the wild.
The structure of feeding behavior in a phytophagous insect (Hylobius abietis)
Authors: Fedderwitz Frauke , Björklund Niklas , Ninkovic Velemir , Nordlander Göran 
Published: September 2023

AbstractAnalysis of the feeding behavior of animals using such a high temporal resolution that meals can be defined may improve our understanding of the mechanisms regulating feeding. Meals can be distinguished in an ethologically meaningful manner by using the ‘meal criterion’, the shortest non‐feeding interval between feeding bouts recognized as meals. However, such a criterion has only been determined for a few insect species. Applying a recent method developed for assessing meal criteria for vertebrates, we determined the meal criterion for Hylobius abietis (L.) (Coleoptera: Curculionidae) based on data from video recordings of single individuals feeding on seedlings of Norway spruce, Picea abies (L.) Karst. (Pinaceae). The pine weevil is an economically important pest insect, because it feeds on the stem bark of planted conifer seedlings. Weevils had 4–5 meals per day. Each meal lasted about 24 min during which about 13 mm2 of bark per meal were removed. Females had longer total meal durations and longer non‐feeding intervals within meals than males. Girdling seedlings did not affect the weevils' feeding properties. The size of meals was significantly correlated with the duration of non‐feeding intervals before and after them. This study is one of few describing the feeding behavior of an insect at a temporal resolution that allows individual meals to be distinguished. With more meal‐related data from insects available, differences in meal properties may be interpreted based on phylogeny, ecology, and physiology. Our results may also assist in the setup and interpretation of studies of plant‐insect interactions, and facilitate the evaluation and development of methods to protect plants against herbivores.
Can non‐native perch (Perca fluviatilis) support native eel populations in a wetland complex?
Authors: Stewart Cohen , Harper Blake , Couper Jayde , Bury Sarah J. , Sabadel Amandine 
Published: September 2023

AbstractIt is well established that non‐native fish can become invasive and outcompete and displace native fish populations. However, little research has explored the potential benefits that non‐native fish may provide to native fish populations. To address this information gap, we examined how the availability of non‐native perch (Perca fluviatilis) as prey could benefit populations of the endemic longfin eel (Anguilla dieffenbachii) and the native shortfin eel (Anguilla australis) in two neighbouring open‐water wetlands in the Rakatu‐Redcliff wetland complex in the Southland region of Aotearoa New Zealand. The Redcliff wetland fish community comprised native fish only, while the Rakatu wetland comprised native fish and non‐native perch. We compared the size, condition, population density, population size structure and diet of eels in these wetlands. While eels were not necessarily larger or better conditioned in Rakatu wetland, their population density was three times higher than the Redcliff wetland, with young‐of‐the‐year perch comprising ca. 40% of their diet. Furthermore, juvenile eel density was four times lower in Redcliff wetland, suggesting that cannibalism may occur at this site to support the existing eel population. Based on our findings, we suggest that freshwater managers consider the predator–prey dynamics of both native and non‐native fish before removing non‐native species, to avoid unintended negative consequences for native predatory fish species.