bluuebunny/crossref_metadata_2025_split huggingface dataset:
Breastfeeding and Lung Function of Children: A Systematic Review and Meta‐Analysis
Authors: Li Ming , Wang Chao , Wang Jiamin , Du Jing , Li Gang 
Published: January 2025

ABSTRACTAimTo evaluate the associations between breastfeeding and lung function among children without known respiratory diseases and to determine the effects of breastfeeding on spirometry.MethodsA systematic search of PubMed, Embase and Cochrane was performed from their inception to 11 Oct 2023 (PROSPERO identifier: CRD42023471664). Observational studies (cohort or cross‐sectional or case–control design) and randomised controlled trials (RCTs) were searched. A random‐effects model meta‐analysis was used to estimate the effect size if significant heterogeneity was detected (p < 0.05 or I2 > 50%); Otherwise, the fixed‐effects model was applied.ResultsIn total, 13 observational studies with 18 152 children were included in the qualitative synthesis, and five studies with 5305 children were included in the meta‐analysis. Compared to children who were never breastfed, those who were breastfed had better FEV1 (β: 19.34 mL; 95% CI: 6.05, 32.64; p = 0.004) and PEF (β: 87.50 mL/s; 95% CI: 27.43, 147.58; p = 0.004). A similar trend was observed for FVC (β: 17.45 mL; 95% CI: −0.43, 35.33; p = 0.06). Furthermore, compared to children breastfed for less than three to four months, those breastfed for three to four months or more had better FEV1 (β: 19.86 mL; 95% CI: 4.21, 35.51; p = 0.01), FVC (β: 31.90 mL; 95% CI: 7.87, 55.93; p = 0.009) and PEF (β: 75.68 mL/s; 95% CI: 24.40, 126.97; p = 0.004).ConclusionThis systematic review and meta‐analysis revealed associations between breastfeeding and improved lung function in children aged 6–16 years. Further high‐quality evidence is needed.
‘Something I've carried with me’: Visibility and vulnerability within the writing journeys of preservice secondary English teachers
Authors: Walters Aisling 
Published: January 2025

AbstractThere is an assumption that English teachers identify as writers. This article explores the stories of three secondary preservice English teachers, their descriptions of their writing experiences and their self‐perceived vulnerabilities around writing. These narratives originated in a broader research study into the writer identities of preservice English teachers. Situated with a qualitative paradigm, the research design aligned with a relativist ontological approach. An interpretivist epistemology led to Interpretative Phenomenological Analysis (IPA). Participants completed qualitative surveys, drew ‘writing rivers’ and reflected on these in semistructured interviews. A story map represents participants' writing journeys over time. Findings suggest that participants associated writing with exposure from an early age. Assessment of writing made participants visible; they experienced negative feedback on their writing as personal criticism and disconnected from writing. Faced with teaching writing as preservice teachers, participants encountered the same pedagogical practices that they found challenging as students. Reflection on their writing histories led participants to explore the source of their writing vulnerabilities and how these were ‘carried’ with them into classroom practice. Findings illustrate how assessment‐driven writing pedagogies can erode the writer identities of young people over time. The research therefore has implications for educational practice in a variety of contexts.
Teachers' choices of and experiences with teaching English literature texts in lower secondary school classes in Trinidad and Tobago
Authors: Seunarinesingh Krishna 
Published: January 2025

AbstractExisting research about English literature texts that are studied at secondary schools in developed countries suggests that teachers' choices can be determined by, for example, school policies, access to books, teachers' reasons for teaching literature, the degree of autonomy they have in choosing text and the influence that canonical literature has in their teaching contexts. Comparable knowledge about teachers' choices of English literature texts, the contexts in which literature is taught and teachers' experiences of teaching the books they choose in Trinidad and Tobago is scarce. To address this, 84 schools were surveyed, and English teachers and heads of department were interviewed. Although many of the findings echoed previous studies conducted in developed countries, several new factors emerged, which may be ascribed to the local context. Chief among these were teachers' preference for multi‐genre anthologies, a high proportion of government schools choosing only one book in Year 8, the popularity of Caribbean texts and the significant impact of economic considerations on text selection. The study discusses the potentially negative consequences of some text selection criteria and questions the applicability of some contemporary research recommendations to Trinidad and Tobago.
Interaction between South American sea lions (Otaria byronia) and trawling fisheries recorded through video cameras
Authors: Apablaza Pedro , Queirolo Dante , Sepúlveda Maritza , Ahumada Mauricio 
Published: January 2025

AbstractThis study investigates the interactions between South American sea lions (Otaria byronia) and trawling fisheries in central‐southern Chile, using underwater video technology. From August 2017 to August 2018, a total of 32 underwater filming sessions were conducted across trawl fisheries targeting Patagonian grenadier (Macruronus magellanicus), nylon shrimp (Heterocarpus reedi), and Chilean hake (Merluccius gayi gayi). The records revealed a total of 15 sea lions, including adult and subadult males and females. These individuals were observed engaging in specific behaviors, such as swimming within the trawl nets and feeding at escape panels of bycatch reduction devices (BRDs). Notably, we documented one subadult male reaching 425 meters. This finding suggests a probable significant adaptability and potential predation behavior at extreme depths. Our findings highlight the importance of further monitoring and research to address conflicts between marine mammal populations and the fishing industry, while enhancing the of conservation strategies to ensure their effectiveness.
Veterans' experiences of somatic experiencing and prolonged exposure therapies for post‐traumatic stress disorder: A qualitative analysis
Authors: Harwood‐Gross Anna , Elias Shir , Lerner Karen , Nacasch Nitsa , Lawi Cathy , Brom Danny , Barak Adi 
Published: January 2025

AbstractPurposeDespite the proliferation of research into evidence based treatment for military PTSD there is little evidence for treatment assignment criterion and military based PTSD still demonstrates low remission rates.MethodThirty participants in a randomized control trial comparing Prolonged Exposure (PE) and Somatic Experiencing (SE) were interviewed on their experiences in therapy and their responses assessed using a descriptive phenomenological analysis approach to delineate the central tenets of the two therapeutic approaches.ResultsResults indicated that participants from both therapies covered themes of the experience of change, the experience of the therapeutic relationship and the therapeutic process. Within these themes, SE and PE participants reported both similar experiences, such as the predominance of physiological or bodily experiences and also described nuanced differences, specifically pertaining to therapy characteristics. SE participants described the process in terms of learning a language, applicable to alternative scenarios and PE participants described the process in terms of conquering exposures in order to achieve respite from symptoms.ConclusionThe current findings have relevance in terms of presenting the key elements of the distinct trauma therapies and determining treatment appropriateness based on desired outcomes. They highlight the commonalities and differences between the patient experience in PE and SE, specifically the relevance of the bodily response, treatment expertise and therapist characteristics in both treatments. Understanding the unique elements of PE, a gold‐standard PTSD treatment and SE, a novel somatic‐based psychotherapy, will allow for better treatment preparation for participants and potentially aid treatment assignment.
Exploring experimental models of prostate cancer in chemoprevention: Oxidative stress as a key pathway to translational research
Authors: Naiki‐Ito Aya , Naiki Taku , Takahashi Satoru 
Published: January 2025

AbstractProstate cancer (PCa) is the second most common cancer in men globally. Its growth is driven by oxidative stress associated with inflammation, aging, and environmental factors, including diet and lifestyle. These factors contribute to multiple stages of PCa progression, including progression to castration‐resistant prostate cancer (CRPC). Therefore, oxidative stress represents an intriguing target for PCa chemoprevention and treatment. In vivo experimental models are crucial for understanding the mechanisms of PCa development, validating chemopreventive and therapeutic approaches, and translating preclinical results into clinical applications. We established a transgenic rat for adenocarcinoma of the prostate (TRAP) model, a transgenic rat that efficiently develops androgen‐dependent adenocarcinoma, pathologically and biologically mimicking human PCa progression, to clarify the mechanisms of tumor progression, including the involvement of oxidative stress, and established a system for screening the chemopreventive effects of agents against PCa. Additionally, we derived a CRPC model from the TRAP model and developed a distant metastasis model, providing a comprehensive multistage rat model of prostate carcinogenesis. This review presents findings on the molecular mechanisms of PCa and the chemopreventive effects of natural compounds with antioxidant properties, such as polyphenols. We additionally described the potential for repositioning existing drugs with antiandrogenic activity for PCa chemoprevention.
Identification of Four New HLA‐B Noncoding Variants by Next‐Generation Sequencing
Authors: Loginova Maria , Obukhov Ivan , Paramonov Igor 
Published: January 2025

ABSTRACTFour novel HLA‐B noncoding variants detected by next‐generation sequencing: HLA‐B*07:02:107, ‐B*08:01:78, ‐B*15:01:89 and ‐B*52:01:58.
Freeze‐dried plasma: Hemostasis and biophysical analyses for damage control resuscitation
Authors: Shoara Aron A. , Singh Kanwal , Peng Henry T. , Moes Katy , Yoo Jeong‐Ah , Sohrabipour Sahar , Singh Sanewal , Huang Rex , Andrisani Peter , Wu Chengliang , Pavenski Katerina , Kim Paul Y. , Trigatti Bernardo , Kretz Colin A. , Rotstein Ori D. , Rhind Shawn G. , Beckett Andrew N. 
Published: January 2025

AbstractBackgroundEffective hemorrhage protocols prioritize immediate hemostatic resuscitation to manage hemorrhagic shock. Prehospital resuscitation using blood products, such as whole blood or alternatively dried plasma in its absence, has the potential to improve outcomes in hemorrhagic shock patients. However, integrating blood products into prehospital care poses substantial logistical challenges due to issues with storage, transport, and administration in field environments.Study Design and MethodsWe utilized hemostatic assays and advanced biophysical techniques, such as calorimetry, infrared spectoscopy, dynamic light scattering, and biolayer interferometry, to compare the functional and structural properties of freeze‐dried plasma (FDP; OctaplasLG Powder, Octapharma AB) with those of fresh plasma controls.ResultsHemostatic characterization of FDP revealed that clot formation properties and coagulation parameters were largely comparable to fresh plasma controls, with some variations observed in Von Willebrand factor–ADAMTS13 axis and fibrinolysis. No change to moisture content of FDP (~1% water content) was observed after 6‐month storage at ambient conditions. Biophysical analyses of FDP during transfusion demonstrated spontaneous exothermic mixing of FDP in plasma, a dilution effect from saline, as well as comparable stability to plasma controls. Quantification of ligand‐binding affinities of platelet receptors activated GPIIbIIIa and GPIbα showed comparable binding properties to plasma controls.ConclusionOur results show that FDP exhibits hemostatic functionality and protein stability on par with fresh plasma, as assessed by novel, highly sensitive techniques. FDP therefore represents a viable alternative to conventional plasma in damage control resuscitation, offering significant logistical and storage advantages for prehospital and remote applications, especially in scenarios where whole blood is unavailable.
HSPG-binding peptide Pep19-2.5 is a potent inhibitor of HPV16 infection
Authors: Mikuličić Snježana , Massenberg Annika , Döring Tatjana , Brandenburg Klaus , Lang Thorsten , Florin Luise 
Published: January 2025

ABSTRACT

Peptide-based therapeutics are gaining attention for their potential to target various viral and host cell factors. One notable example is Pep19-2.5 (Aspidasept), a synthetic anti-lipopolysaccharide peptide that binds to heparan sulfate proteoglycans (HSPGs) and has demonstrated inhibitory effects against certain bacteria and enveloped viruses. This study explores, for the first time, the effectiveness of Pep19-2.5 against a non-enveloped virus, using pseudoviruses of the oncogenic human papillomavirus type 16 (HPV16) as a model. HPV16 infects epithelial cells of the skin and mucosa by using multiple cell surface receptors with initial attachment to HSPGs. Pharmacological inhibition with Pep19-2.5 in HeLa and HaCaT cells resulted in a concentration-dependent reduction of HPV16 PsV infection, with near-complete blockade observed at higher concentrations. The half-maximal inhibitory concentration (IC
50
) was determined to be 116 nM in HeLa cells and 183 nM in HaCaT cells, highlighting its potent antiviral activity. Our results demonstrate that Pep19-2.5 not only inhibits HPV16 PsV binding to the cell surface but also significantly reduces infection when administered post-binding. Imaging analyses revealed Pep19-2.5-dependent release of large cell-associated crowds of viral particles, suggesting interference with the transfer to secondary receptor molecules. This was corroborated by the effectiveness of Pep19-2.5 in an HSPG-negative cell line, indicating that the peptide disrupts virus binding to both primary and secondary interaction partners. Based on these findings, we propose that the antimicrobial effect of Pep19-2.5 is not limited to HSPG-dependent infections. Additionally, Pep19-2.5 may be a valuable tool for dissecting specific steps in the viral entry process.
Understanding phage BX-1 resistance in
Vibrio alginolyticus
AP-1 and the role of quorum-sensing regulation
Authors: Li Xiaoyu , Liu Xin , Ma Tianyi , Su Haochen , Sui Bingrui , Wang Lili , Murtaza Bilal , Xu Yongping , Li Na , Tan Demeng 
Published: January 2025

ABSTRACT

The marine ecosystem is characterized by a rich diversity of bacterial hosts and their phages. The propagation of phages is primarily limited by their ability to adsorb to host cells and is further challenged by various bacterial defense mechanisms. To fully realize the potential of phage therapy in aquaculture, a comprehensive understanding of phage-host interactions and their regulation is essential. In this study, we isolated a novel
Myoviridae
phage, BX-1, capable of infecting
Vibrio alginolyticus
AP-1, and characterized its resistant mutants. We elucidated the essential role of the bacterial cellulose biosynthesis-related gene
bcsE
, which functions as a cyclic di-GMP-binding protein, in influencing host susceptibility to phage BX-1. Interestingly, Congo Red, Calcofluor White staining, and cellulose content assays indicated that deletion of
bcsE
in strain AP-1 does not completely abolish cellulose production, suggesting that
bcsE
is not essential for bacterial cellulose synthesis. Furthermore, investigating the signaling molecules that regulate phage-host interactions, we find that in a high cell density state (Δ
luxO
), bacterial cells upregulate their susceptibility to phage BX-1, which leads to a rapid development of resistance. Conversely, cells in a low-density state (Δ
hapR
) exhibit reduced susceptibility to phage BX-1 while still producing comparable phage progenies. This population density-dependent response is primarily enhanced by the predicted quorum-sensing autoinducer CAI-1, synthesized by the gene
cqsA
. Collectively, our findings reveal the intricate dynamics of phage-host interactions, adding a new layer of complexity to our understanding of phage receptor regulations.

IMPORTANCE

Phage therapy has garnered significant attention as a promising solution to antibiotic resistance in aquaculture. However, its application is hindered by a limited understanding of the genotypic and phenotypic dynamics governing phage-host interactions. Bacteria have developed various defense mechanisms against phages, such as mutations in phage receptors. In this study, we demonstrate that the bacterial cellulose biosynthesis-related gene
bcsE
plays a crucial role in determining susceptibility to phage BX-1, while quorum-sensing (QS) systems significantly influence collective phage-related behaviors. By characterizing the mechanisms of phage resistance and the regulatory role of QS in susceptibility, our findings enhance the understanding of phage-host interactions and pave the way for more effective phage therapy applications. Collectively, these insights illuminate the evolutionary complexities of phage-defense systems and the broader strategies that bacteria employ to coexist with phages.
Rare occurrence of upper-limb ischaemia necessitating transhumeral amputation following axillary arterial line placement
Authors: Boland Allison Clare , Joseph-Mofford Genevieve , Pino Cassandra , Levine Diane , Tennenberg Steven D , Vaidya Rahul 
Published: January 2025

Arterial cannulation, commonly performed in the radial artery, is a widely used method for continuous blood pressure monitoring. Occasionally, the axillary artery is used as an alternate site of cannulation. However, complications like occlusion can lead to adverse events and severe outcomes. We present a case of axillary arterial line placement resulting in ischaemic limb injury and amputation, highlighting the need to recognise risk factors.A woman in her mid-60s underwent a lobectomy for adenocarcinoma of the lung and suffered postoperative complications resulting from arterial line placement. Despite interventions, she developed progressive ischaemia of her left upper limb, necessitating amputation. This case highlights the risks associated with arterial catheterisation and potential adverse outcomes, offering recommendations for arterial line placement in high-risk patients.
Disparities in dolutegravir utilisation in children, adolescents and young adults (0–24 years) living with HIV. An analysis of the IeDEA Pediatric West African cohort
Authors: Desmonde Sophie , Dame Joycelyn , Malateste Karen , David Agatha , Amorissani-Folquet Madeleine , N'Gbeche Sylvie , Sylla Mariam , Takassi Elom , Eboua François Tanoh , Kouakou Kouadio , Bagnan Tossa Lehila , Yonaba Caroline , Leroy Valeriane 
Published: January 2025

IntroductionWe describe the 24-month incidence of Dolutegravir (DTG)-containing antiretroviral treatment (ART) initiation since its introduction in 2019 in West Africa.MethodsWe included all patients aged 0–24 years on ART from nine clinics in Côte d’Ivoire (n=4), Ghana, Nigeria, Mali, Benin, and Burkina Faso. Baseline varied by clinic and was defined as date of first DTG prescription; patients were followed up until database closure/death/loss to follow-up (LTFU, no visit ≥7 months), whichever came first. We computed the cumulative incidence function for DTG initiation; associated factors were explored in a shared frailty model, accounting for clinic heterogeneity.ResultsSince 2019, 3350 patients were included; 47.2% were female; 78.9% had been on ART ≥12 months. Median baseline age was 12.5 years (IQR 8.4–15.8). Median follow-up was 14 months (IQR 7–22). The overall cumulative incidence of DTG initiation reached 22.7% (95% CI 21.3 to 24.2) and 56.4% (95% CI 54.4 to 58.4) at 12 and 24 months, respectively. In univariate analyses, those aged <5 years and female were overall less likely to switch. Adjusted on ART line and available viral load (VL) at baseline, females aged >10 years were less likely to initiate DTG compared with males of the same age (adjusted HR among 10–14 years: 0.62, 95% CI 0.54 to 0.72; among ≥15 years: 0.43, 95% CI 0.36 to 0.50), as were those with detectable VL (>50 copies/mL) compared with those in viral suppression (aHR 0.86, 95% CI 0.77 to 0.97) and those on PIs compared with those on non-nucleoside reverse-transcriptase inhibitors (aHR after 12 months of roll-out: 0.75, 95% CI 0.65 to 0.86).ConclusionPaediatric DTG uptake was incomplete and unequitable in west African settings: DTG use was least likely in children <5 years, females ≥10 years and those with detectable VL. Maintained monitoring and support of treatment practices is required to better ensure universal and equal uptake.
Characterization of individuals achieving type 2 diabetes remission in real-world settings: bridging clinical evidence and patient experiences
Authors: Marcotte-Chénard Alexis , Oliveira Barbara , Rahman Tahmina , McKelvey Sean , Elliott Tom , Rocha Paula , Mackay Dylan , Little Jonathan P. 
Published: January 2025

The objectives of the study were to (1) describe characteristics and lifestyle factors of individuals who have achieved type 2 diabetes (T2D) remission (sub-diabetes glucose levels without glucose-lowering medications for ≥3 months) through changes to diet and exercise behaviour in real-world settings; (2) investigate continuous glucose monitoring (CGM) profiles of these individuals and explore how dietary pattern may influence glucose regulation metrics. This cross-sectional study recruited individuals living with T2D who achieved remission via changes to diet or exercise behaviours. Various questionnaires were used to assess overall health and participants wore a blinded CGM for 14 days to assess glucose profiles and filled out 3-day food records. A total of 21 adults (57 ± 8 years of age) who were recently diagnosed with T2D (4 ± 3 years) with a A1c of 5.7 ± 0.4% volunteered to participate. Participants achieved remission through various means (e.g., combination of diet and exercxise/physical activity) and self-reported following different diets, including 52% following a low-carbohydrate or very low carbohydrate diet, 14% following a “ketovore/carnivore” diet, 10% using a meal replacement diet, 5% following Weight Watcher’s diet, and 19% no defined dietary pattern. The 24 h average CGM glucose value was 5.0 [4.8–5.6] mmol/L (median [IQR]) with 92 [85–97]% of time spent in range (between 4.0 and 9.9 mmol/L). The 24 h average CGM glucose ( r = 0.692; P = 0.001) and A1c ( r = 0.470; P = 0.049) were correlated with the daily percentage of energy intake from carbohydrate. Remission of T2D appears achievable through various means, including adoption of different dietary approaches and a more active lifestyle underpinning the importance of a patient-centred care.