bluuebunny/crossref_metadata_2025_split huggingface dataset:
Epigenetic modifications regulate cultivar-specific root development and metabolic adaptation to nitrogen availability in wheat
Authors: Xiao Jun , Zhang Hao , Jin Zhiyuan , Cui Fa , Zhao Long , Zhang Xiaoyu , Chen Jinchao , Zhang Jing , Li Yanyan , Li Yongpeng , Niu Yanxiao , Zhang Wenli , Gao Caixia , Fu Xiangdong , Tong Yiping , Wang Lei , Ling HongQing , Li Junming 
Published: April 2023

Abstract
The breeding of crops with improved nitrogen use efficiency (NUE) is crucial for sustainable agriculture. Despite its importance, the way in which epigenetic modifications regulate cultivar-specific responses to low nitrogen (LN) constraints is not yet well understood. Here, we analyzed the chromatin landscapes in the roots, leaves, and seeds of two wheat cultivars (KN9204 and J411) that differ radically in NUE under varied nitrogen conditions. The chromatin regions responsible for regulating gene transcription exhibited clear cultivar-specificity between the two cultivars, and the regulation of nitrogen metabolism genes (NMGs) was closely linked to variation in histone modification levels instead of differences in DNA sequence. We also found that cultivar-specific histone modification regions contribute to the genetic regulation of NUE-related traits, such as the QTL locus of maximum root length of qMRL-7B. Additionally, LN-induced H3K27ac and H3K27me3 dynamics enhanced root growth more significantly in KN9204, while strengthened the nitrogen uptake system remarkably in J411. Evidence from histone deacetylase inhibitor treatment and transgenic plants with loss function of the H3K27me3 methyltransferase further showed that changes in epigenetic modifications can alter the strategy for root development and nitrogen uptake in response to LN constraint. Taken together, our data highlight the importance of epigenetic regulation in mediating cultivar-specific adaptation to LN in wheat.
Activated Partial Thromboplastin Time-based Clot Waveform Analysis: A Potential for Application in Acute Myocardial Infarction and Its Complications
Authors: Ng Chen Lin , Uy Felix Maverick , Cheong May Anne , Wong Wan Hui , Lau Yee How , Ng Heng Joo , Yeo Khung Keong , Tan Chuen Wen 
Published: April 2023

Abstract
Background: Activated partial thromboplastin time (aPTT)-based clot waveform analysis (CWA) is a plasma-based global haemostatic assay. Elevated CWA parameters have been associated with hypercoagulability in venous thromboembolism, but its role in arterial thrombotic disease is uncertain. This study aims to explore the relationship between aPTT-based CWA and acute myocardial infarction (AMI) and its complications.
Methods: This is a retrospective case-control study that included patients with AMI who underwent emergency cardiac catheterisation and control patients who underwent elective orthopaedic and urological procedures. The pre-procedural aPTT and CWA parameters – min1, min2 and max2 – of AMI patients were compared against those of controls.
Results: Compared to controls (N=109), patients with AMI (N=214) had shorter aPTT (26.7±3.3s vs 27.9±1.7s, P<0.001) and higher CWA parameters (min1: 6.11±1.40%/s vs 5.58±1.14%/s; min2: 0.98±0.23%/s2 vs 0.90±0.19%/s2; max2: 0.81±0.20%/s2 vs 0.74±0.16%/s2, all P≤0.001). Elevated CWA parameters, defined as having CWA values above their respective reference ranges, were associated with the occurrence of AMI, with odds ratio (OR) of 2.06 [95% confidence intervals (CI):1.10–3.86], 2.23 (95% CI:1.18–4.24) and 2.01 (95% CI:1.07–3.77) for min1, min2 and max2, respectively. Similarly, elevated min1 and min2 were both individually associated with the presence of adverse outcomes of AMI with ORs of 2.63 (95% CI:1.24–5.59).
Conclusions: Patients with AMI had significantly increased CWA parameters. Elevated aPTT-based CWA parameters are significantly associated with the occurrence of AMI and its complications. Potential utility of CWA as risk and prognostic markers for AMI warrants future works.
The mismatch between experimental and computational fluid dynamics analyses for magnetic surface microrollers
Authors: Bozuyuk Ugur , Ozturk Hakancan , Sitti Metin 
Published: April 2023

Abstract
Magnetically actuated Janus surface microrollers is a promising microrobotic platform with numerous potential biomedical engineering applications. While the locomotion models based on a "rotating sphere on a nearby wall" can be adapted to surface microrollers, real-world dynamics may differ from the proposed theories/simulations. In this study, we examine the locomotion efficiency of surface microrollers with diameters of 5, 10, 25, and 50 µm and demonstrate that computational fluid dynamics simulations cannot accurately capture locomotion characteristics for different sizes of microrollers. Specifically, we observe a significant mismatch between lift forces predicted by simulations and opposite balancing forces, particularly for smaller microrollers. We propose the existence of an unaccounted force component in the direction of lift, which was not included in the computational fluid dynamics simulations. Overall, our findings provide a deeper understanding of the physical mechanisms underlying surface microroller locomotion and have important implications for future applications in biomedical engineering.
Immune response of the Almaco jack (Seriola rivoliana) against the infestation with Neobenedenia sp. in three cultivated temperatures
Authors: Valles-Vega Isabel , Pérez-Urbiola Juan Carlos , Tovar-Ramírez Dariel , Graham Andrea L. , Sicard-González Teresa , Ascencio Felipe 
Published: April 2023

Abstract
The Almaco jack (Seriola rivoliana) is a marine fish maintained in mariculture systems and frequently infested by monogenean parasites like Neobenedenia sp. Severe infestations can lead to high mortalities and economic losses for farmers. This study evaluated the effects of temperature on the immune response on Almaco jack infested with Neobenedenia sp. We exposed infested fishes at temperatures of 20°C, 24°C and 30°C for 20 days and took samples of different tissues at the beginning of the experiment, and after 3 and 20 days. The tissues considered were the skin, thymus, cephalic kidney, and spleen to evaluate the relative gene expression of different genes: Hsp70, IgM, IL-1β, IL-10, and MyD88. Our results showed an increase in IL-1β gene expression in the skin after 20 days of infestation but no significant effect of temperature on gene expression, despite increases in infestation rates with temperature. These results show that the parasite infestation induced a local response in the skin, but that temperature has a limited direct effect on the immune system of Almaco jack.
Texture-based brain networks for characterization of healthy subjects from MRI
Authors: Silveira Rafael Vinícius , Li Li Min , Castellano Gabriela 
Published: April 2023

Abstract
Background: Brain networks have been widely used to study the relationships between brain regions based on their dynamics using, e.g. fMRI or EEG, and to characterize their real physical connections using DTI. However, few studies have investigated brain networks derived from structural properties; and those have been based on cortical thickness or gray matter volume.
Purpose: The main objective of this work was to investigate the feasibility of obtaining useful information from brain networks derived from structural MRI, using texture features. We also wanted to verify if texture brain networks had any relation with established functional networks.
Methods: T1-MR images were segmented using AAL and texture parameters from the gray-level co-occurrence matrix were computed for each region, for 760 subjects. Individual texture networks were used to evaluate the structural connections between regions of well-established functional networks; assess possible gender differences; investigate the dependence of texture network parameters with age; and single out brain regions with different texture-network characteristics.
Results and discussion: Although around 70% of texture connections between regions belonging to the default mode, attention, and visual network were greater than the mean connection value, this effect was small (only between 7% and 15% of these connections were larger than one standard deviation), implying that texture-based morphology does not seem to subside function. This differs from cortical thickness-based morphology, which has been shown to relate to functional networks. Eighty out of 86 evaluated regions showed significant (ANCOVA, p<0.05) differences between genders. Forty-two out of 86 regions showed significant (ANCOVA, p<0.05) dependence with age; however, the R2 indicates that this is not a linear relation. Thalamus and putamen showed a very unique texture-wise structure compared to other analyzed regions.
Conclusions: Texture networks were able to provide useful information regarding gender and age-related differences, as well as for singling out specific brain regions. We did not find a morphological texture-based subsidy for the evaluated functional brain networks. In the future, this approach will be extended to neurological patients to investigate the possibility of extracting biomarkers to help monitor disease evolution or treatment effectiveness.
Mechanical properties of rooted soil under freeze-thaw cycles and extended binary medium model
Authors: Luo Wei , Xiang Bo , Liu Enlong , Zhao Haisong , Wu Kai , He Yunyong 
Published: April 2023

Abstract
In seasonally frozen soil, soil sometimes is affected by freeze-thaw cycles and root systems. In order to study its mechanical characteristics, a series of consolidation drained triaxial tests under different confining pressures (25,50,100,200 kPa), different freeze-thaw cycles (N = 0,1,5,15) and different root-containing conditions (r = 0,1,3) were carried out. The test results show that the specimens exhibit strain softening behavior and volumetric dilatancy phenomena and shear failure under lower confining pressure, and strain hardening and volumetric contraction, bulging failure under higher confining pressure. With the increase of freeze-thaw cycles, the bearing capacity of the sample decreases and the volume strain increases. With the increase of volume ration of roots in the sample, the bearing capacity increases and the volume strain decreases. Based on the binary medium model, the soil is abstracted into bonded elements and frictional element. At the same time, the bonded elements is transformed into frictional element when the bonded elements is broken during the loading process. Also, the root is abstracted into another non-destructive bonded elements material, which bears the load together. The linear elastic constitutive model was used for root and bonded elements, and the double-hardening model was used for friction elements. Considering the influence of freeze-thaw cycles, the extended binary model was derived here. Finally, the experimental results show that the predicted results of this model are in good agreement with the experimental results, and the new model can relatively well simulate the strain softening and volumetric dilatancy phenomena.
Analysis of Dynamic Electromagnetic Force in Synchronous Reluctance Motor Accounting for Rotor Eccentric
Authors: Tao Dajun , Pan Bo , Zhou Kailiang , Han Jichao , Lin Peng , Ge Baojun 
Published: April 2023

Abstract
Aiming at the influence of eccentric faults on the performance of synchronous reluctance motor (SynRM), the distribution characteristics and change rules of dynamic electromagnetic force before and after eccentric faults of SynRM were studied. A dynamic electromagnetic force calculation model combining eccentric fault finite element method and Maxwell tensor method is established under the influence of magnetic saturation. The dynamic electro-magnetic forces before and after the eccentric fault of the rotor surface, rotor magnetic ribs, and stator tooth wall are calculated, and the differences in the electromagnetic force distribution are analyzed in detail. The analysis methods and results of electromagnetic force provide a reference for the design and structure optimization of SynRM, and it also presents a theoretical guarantee for the smooth and orderly operation of SynRM.
The causal relationship between gut microbiota and immune skin diseases: a bidirectional Mendelian randomization
Authors: feng fei , Li Ruicheng , Tin Rui , Wu Xueyi , Zhang Nannan , Nie Zhenhua 
Published: April 2023

Abstract
Background Increasing evidence suggests that alterations in gut microbiota are associated with a variety of skin diseases. However, whether this association reflects a causal relationship remains unknown. We aimed to reveal the causal relationship between gut microbiota and skin diseases, including psoriasis, atopic dermatitis, acne, and lichen planus.Methods We obtained full genetic association summary data for gut flora, psoriasis, atopic dermatitis, acne, and lichen planus from public databases and used three methods, mainly inverse variance weighting, to analyze the causal relationships between gut flora and these skin diseases using bidirectional Mendelian randomization, as well as sensitivity and stability analysis of the results using multiple methods.Results The results showed that there were 5 associated genera in the psoriasis group, 10 associated genera were obtained in the atopic dermatitis group, a total of 8 associated genera in the acne group, and 6 associated genera in the lichen planus group. The results corrected for false discovery rate showed that Eubacteriumfissicatena(p = 7.10E-05, OR = 1.44,95%CI: 1.20–1.72) and Lactococcus(p = 4.90E-04, OR = 1.37,95%CI: 1.15–1.65) and psoriasis, and Coprococcus-3(p = 0.001, OR = 2.39,95%CI: 1.41–4.03) and acne still showed a causal relationship. In contrast, in the reverse Mendelian randomization results, there was no evidence of an association between these skin diseases and intestinal flora.Conclusion We demonstrated a causal relationship between gut microbiota and immune skin diseases and provide a new therapeutic perspective for the study of immune diseases: targeted modulation of dysregulation of specific bacterial taxa to prevent and treat psoriasis, atopic dermatitis, acne, and lichen planus.
The constant force control based on an optimized environmental model for the robotic lapping of curved surface
Authors: Shi Yuxin , Wang Jinghang , He Xiangbo , Peng Yunfeng 
Published: April 2023

Abstract
During the curved surface lapping processing by robot, the surface quality of the processed workpieces cannot be fully guaranteed because of the uncontrollability of lapping contact force. Therefore, the accurate control of the contact force between the tool and the workpiece is important to improve the processing quality. This paper proposes an impedance control strategy based on an optimized environmental model to address the serious constraints brought by the uncertainty of the environmental model in impedance control on the compensation accuracy of contact force, and constructed a contact environmental model of elastic abrasives based on neural network algorithm, which improved the compensation accuracy of contact force through iterative iteration and prediction between the environmental model and the impedance model. Through comparison experiments, it is found that compared with the unoptimized method, the force control tracking error is reduced by 60.9%, the contact force variance is reduced by 25.7%, the contact force error during curved surface force control lapping process is reduced by 58.3% and the contact force variance is reduced by 52.5%, and the workpiece roughness Ra value is reduced by 37.2%. Therefore, the robotic constant force control system based on optimized environmental model can achieve a better force control effect, which can realize accurate and stable control of contact force during curved surface lapping process by robot, and has certain potential for engineering application.
Exploring the Solar System through Hydrogen Line Sprectum Radio Data Analysis
Authors: Dutta Pritam , Saraswat Rushil 
Published: April 2023

Abstract
This research paper provides an in-depth analysis of the solar system usinga technique called hydrogen line radio data analysis. This method involvesstudying the spectral line emitted by neutral hydrogen atoms, known as thehydrogen line or the 21-centimeter line. Astronomers use this technique toexamine the distribution and characteristics of hydrogen gas within the universe.The researchers examined hydrogen line radio data from a range of sources inthis study to investigate the properties of hydrogen gas throughout the solarsystem. The emphasis was on identifying the presence of hydrogen gas in thesolar system's planets, moons, and other objects. Their findings revealed thathydrogen gas is found in numerous solar system objects, including the big planetsJupiter, Saturn, Uranus, and Neptune, as well as their moons. Furthermore,the researchers discovered hydrogen gas in comets and asteroids, indicatingthe presence of water, ice, and other volatile molecules. Overall, this researchdemonstrates the usefulness of hydrogen line radio data analysis in exploringthe properties of hydrogen gas within the solar system. It provides importantinsights into the composition and evolution of the solar system, and could alsobe applied to studying planetary systems beyond our own.
ERK Hyperactivation Serves as a Unified Mechanism of Escape in Intrinsic and Acquired CDK4/6 Inhibitor Resistance in Acral Lentiginous Melanoma
Authors: Rebecca Vito , Jagirdar Kasturee , Portuallo Marie , Wei Meihan , Wilhide Matthew , Bravo Jeremy , Robertson Bailey , Alicea Gretchen , Aguh Crsytal , Xiao Min , Godok Tetiana , Fingerman Dylan , Brown Gregory , Herlyn Meenhard , Guo Brian , Toska Eneda , Zabransky Daniel , Wubbenhorst Bradley , Nathanson Katherine , Kwatra Shawn , Goyal Yogesh , Ji Hongkai , Liu Qin 
Published: April 2023

Abstract
Patients with metastatic acral lentiginous melanoma (ALM) suffer worse outcomes relative to patients with other forms of cutaneous melanoma (CM), and do not benefit as well to approved melanoma therapies. Identification of cyclin-dependent kinase 4 and 6 (CDK4/6) pathway gene alterations in > 60% of ALMs has led to clinical trials of the CDK4/6 inhibitor (CDK4i/6i) palbociclib for ALM; however, median progression free survival with CDK4i/6i treatment was only 2.2 months, suggesting existence of resistance mechanisms. Therapy resistance in ALM remains poorly understood; here we report hyperactivation of MAPK signaling and elevated cyclin D1 expression are a unified mechanism of both intrinsic and acquired CDK4i/6i resistance. MEK and/or ERK inhibition increases CDK4i/6i efficacy in a patient-derived xenograft (PDX) model of ALM and promotes a defective DNA repair, cell cycle arrested and apoptotic program. Notably, gene alterations poorly correlate with protein expression of cell cycle proteins in ALM or efficacy of CDK4i/6i, urging additional strategies when stratifying patients for CDK4i/6i trial inclusion. Concurrent targeting of the MAPK pathway and CDK4/6 represents a new approach to improve outcomes for patients with advanced ALM.
The maximum augmented Zagreb index for interval graphs with edge-connectivity one
Authors: Malik Mehar Ali , Butt Muhammad Yasir , Asif Unaza , Irshad Alveena 
Published: April 2023

Abstract
The augmented Zagreb index (AZI) of graph is defined as the sum of weights of all edges uv of a graph G, where the weight of edge uv is calculated as (d(u)d(v) / d(u)+d(v)−2)3. In this paper, we examine the (maximum) AZI for graphs with edge connectivity one and examine the relative extremal graphs.
MSC: 5C35.
Effects of Orange Essential Oil on Some Parameters of Dulce De Leche Prepared with Plant-Based Hazelnut Milk and Cow's Milk Combinations
Authors: Yangılar Filiz 
Published: April 2023

Abstract
This study evaluated the use of orange essential oil (OEO) in conjunction with cow milk (CM) and hazelnut milk (HM) to fortify dulce de leche, due to the increased interest in plant-based foods in recent years. The "dulce de leche" (DL) formed from CM and HM, as well as CM:HM mixing rations of 100:0, 75:25, 50:50, and 25:75, are produced for this purpose and physicochemically characterized. When milk jam samples were made using hazelnut milk and orange essential oil, the values for dry matter, titration acidity, protein, oil, and ash were statistically significant (p<0.05). It was determined that the jams made with various milk combinations and orange essential oil had rheological characteristics with K values between 28.17 and 31.47, n values between 0.21 and 0.33, and R2 values between 0.96-0.99. 5-Hydroxymethylfurfural (HMF) values ranged from 10.04 to 44.65 m/kg, L* values ranged from 35.3-53.2, a* values ranged from -0.84 to -1.11, and b* values ranged from 3.78 to 11.3. The interaction between storage days was found to be statistically significant in terms of texture values (p<0.05). When the sensory evaluation findings were statistically evaluated, the general acceptance scores in the A and C samples were determined to be high.
Etiological characteristics and appropriate empiric antibiotic strategies of multidrug-resistant pathogens in patients requiring extracorporeal membrane oxygenation: a retrospective cohort study
Authors: Gao Xinjing , Wang Wenjiao 
Published: April 2023

Abstract
Background: Delayed treatments of MDR infections in patients receiving extracorporeal membrane oxygenation (ECMO) supports associated with increased mortality. But, there was currently no relevant references available to guide early anti-infective treatments for them. The purpose of our study was to clarify etiological characteristics and empiric antibiotic strategies of MDR pathogens in ECMO patients.
Methods: The clinical data of 104 ECMO patients in Tianjin third central hospital were retrospectively collected from January 2014 to December 2022. There were 61 and 29 adult patients supported by veno-arterial (VA)-ECMO or veno-venous (VV)-ECMO for > 24 hours enrolled. Patients were divided into “group R” and “group N” by with or without MDR infections. Clinical characteristics of enrolled patients were collected. The data of MDR pathogens and anti-infection treatments against them were summarized.
Results: The rates of MDRinfections were 50.8% and 79.3% in VA-ECMO and VV-ECMO patients respectively. ECMO patients in group R presented poor prognostic indicators as expected. MDR pathogens detected were mainly MDR Gram-negative bacteria (MDR-GNB) (91.6% in VA-ECMO patients and 92.5% in VV-ECMO patients, respectively). Sputum was the main source of MDR. There were 72.9% and 80% of MDR pathogens deprived from sputum in VA-ECMO and VV-ECMO patients respectively. MDR pathogens generally showed high drug-resistant prevalence to routine antibiotics, and anti-infection treatments against them were not optimistic. Cephalosporins, meropenem, linezolid and tigecycline were most selected antibiotics according to antimicrobial susceptibility tests in our study.
Conclusion: ECMO patients had high incidence of MDR infection, which mostly induced by MDR-GNB from sputum. Cephalosporins, meropenem, linezolid and tigecycline were appropriate empiric antibiotics against MDR pathogens in ECMO patients according to our study.