bluuebunny/crossref_metadata_2025_split huggingface dataset:
Meal-specific dietary patterns and biomarkers of insulin resistance in a sample of Iranian adults: a cross-sectional study
Authors: Lesani Azadeh , Jayedi Ahmad , Karimi Mehrdad , Djafarian Kurosh , Barkhidarian Bahareh , Akbarzade Zahra , Shab-Bidar Sakineh 
Published: May 2023

AbstractCurrent research emphasizes the habitual dietary pattern without differentiating eating occasions. We aimed to assess meal-specific dietary patterns and insulin resistance indicators. This cross-sectional study was conducted on 825 Iranian adults. Dietary data were recorded by three 24-h dietary recalls. Dietary patterns were identified using principal component analysis (PCA) on main meals and an afternoon snack. Anthropometric measurements, blood pressure, and laboratory investigation, fasting plasma glucose (FPG), triglyceride, insulin, c-reactive protein (CRP) were done. Homeostatic model assessment for insulin resistance and sensitivity (HOMA-IR and HOMA-IS), Triglycerides and glucose (TyG-index), and Lipid accommodation product index were calculated. We used multivariate analysis of variance (MANOVA) analysis. Two major dietary patterns at the main meals and the afternoon were identified. Higher adherence to “Bread, vegetable, and cheese” dietary pattern at breakfast was related to lower FPG, and “Oil, egg, and cereals” showed a direct association with body mass index, FPG, and TyG-index at breakfast. The “Western” pattern in lunch and dinner directly related to waist circumference (WC) and body mass index, however, it was inversely related to HOMA-IS. This pattern at dinner was related to higher CRP. Higher adherence to “bread, cereals, and oil” pattern at afternoon snack was associated with a lower WC. These results indicated that unhealthy meal-specific dietary patterns are associated with a greater chance of obesity and insulin resistance risk. However, “Bread, vegetable, and cheese” dietary pattern at breakfast was related to lower FPG, and “bread, cereals, and oil” pattern in the afternoon was associated with lower WC.
Co-application of Mycorrhiza and methyl jasmonate regulates morpho-physiological and antioxidant responses of Crocus sativus (Saffron) under salinity stress conditions
Authors: Hamidian Mohammad , Movahhedi-Dehnavi Mohsen , Sayyed R. Z. , Almalki Waleed Hassan , Gafur Abdul , Fazeli-Nasab Bahman 
Published: May 2023

AbstractSalinity stress is the second most devastating abiotic factor limiting plant growth and yields. Climate changes have significantly increased salinity levels of soil. Besides improving the physiological responses under stress conditions, jasmonates modulate Mycorrhiza—Plant relationships. The present study aimed to evaluate the effects of methyl jasmonate (MeJ) andFunneliformis mosseae(Arbuscular mycorrhizal (AM) on morphology and improving antioxidant mechanisms inCrocus sativusL. under salinity stress. After inoculation with AM, pre-treatedC. sativuscorms with MeJ were grown under low, moderate, and severe salinity stress. Intense salinity levels damaged the corm, root, total leaf dry weight, and area. Salinities up to 50 mM increased Proline content and Polyphenol oxidase (PPO) activity, but MeJ increased this trend in proline. Generally, MeJ increased anthocyanins, total soluble sugars, and PPO. Total chlorophyll and superoxide dismutase (SOD) activity increased by salinity. The maximum catalase and SOD activities in + MeJ + AM were 50 and 125 mM, respectively, and the maximum total chlorophyll in –MeJ + AM treatment was 75 mM. Although 20 and 50 mM increased plant growth, using mycorrhiza and jasmonate enhanced this trend. Moreover, these treatments reduced the damage of 75 and 100 mM salinity stress. Using MeJ and AM can improve the growth of saffron under various ranges of salinity stress levels; however, in severe levels like 120 mM, this phytohormone andF. mosseaeeffects on saffron could be adverse.
Association of gamma-glutamyl transferase variability with risk of venous thrombosis
Authors: Chang Yoonkyung , Lee Heajung , Song Tae-Jin 
Published: May 2023

AbstractGamma-glutamyl transferase (GGT) is a biomarker of inflammation, and is known to be associated with stroke and atrial fibrillation. Venous thromboembolism (VT), a not uncommon thrombotic disorder, shares similar mechanisms with other thrombotic disorders including these stroke and atrial fibrillation. Given these associations, we intended to investigate the potential association between variability in GGT and VT. The study included data from the National Health Insurance Service-Health Screening Cohort, comprising 1,085,105 participants with health examinations 3 or more times from 2003 to 2008. Variability indexes were the coefficient of variation, standard deviation, and variability independent of the mean. The occurrence of venous thromboembolism (VT) was defined with more than one claim of the following ICD-10 codes: deep VT (I80.2–80.3), pulmonary thromboembolism (I26), intraabdominal venous thrombosis (I81, I82.2, I82.3), or other VT (I82.8, I82.9). To determine the relationship of quartiles of GGT with incident VT risk, Kaplan–Meier survival curve and logrank test were used. Cox’s proportional hazard regression was used to investigate the risk of VT occurrence by GGT quartile (Q1–Q4). A total of 1,085,105 subjects were incorporated in the analysis, and the average follow-up was 12.4 years (interquartile range 12.2–12.6). VT occurred in 11,769 (1.08%) patients. The GGT level was measured 5,707,768 times in this stud. Multivariable analysis showed that GGT variability were positively associated with the occurrence of VT. Compared to the Q1, the Q4 showed an adjusted HR of 1.15 (95% CI 1.09–1.21, p < 0.001) when using coefficient of variation, 1.24 (95% CI 1.17–1.31, p < 0.001) when using standard deviation, and 1.10 (95% CI 1.05–1.16, p < 0.001) when using variability independent of the mean. Increased variability of GGT may be related to an increased risk of VT. Maintaining a stable GGT level would be beneficial in reducing the risk of VT.
Enhanced design of multiplexed coded masks for Fresnel incoherent correlation holography
Authors: Gopinath Shivasubramanian , Bleahu Andrei , Kahro Tauno , John Francis Rajeswary Aravind Simon , Kumar Ravi , Kukli Kaupo , Tamm Aile , Rosen Joseph , Anand Vijayakumar 
Published: May 2023

AbstractFresnel incoherent correlation holography (FINCH) is a well-established incoherent digital holography technique. In FINCH, light from an object point splits into two, differently modulated using two diffractive lenses with different focal distances and interfered to form a self-interference hologram. The hologram numerically back propagates to reconstruct the image of the object at different depths. FINCH, in the inline configuration, requires at least three camera shots with different phase shifts between the two interfering beams followed by superposition to obtain a complex hologram that can be used to reconstruct an object’s image without the twin image and bias terms. In general, FINCH is implemented using an active device, such as a spatial light modulator, to display the diffractive lenses. The first version of FINCH used a phase mask generated by random multiplexing of two diffractive lenses, which resulted in high reconstruction noise. Therefore, a polarization multiplexing method was later developed to suppress the reconstruction noise at the expense of some power loss. In this study, a novel computational algorithm based on the Gerchberg-Saxton algorithm (GSA) called transport of amplitude into phase (TAP-GSA) was developed for FINCH to design multiplexed phase masks with high light throughput and low reconstruction noise. The simulation and optical experiments demonstrate a power efficiency improvement of ~ 150 and ~ 200% in the new method in comparison to random multiplexing and polarization multiplexing, respectively. The SNR of the proposed method is better than that of random multiplexing in all tested cases but lower than that of the polarization multiplexing method.
Thermoelasticity of ice explains widespread damage in dripstone caves during glacial periods
Authors: Spötl Christoph , Jarosch Alexander H. , Saxer Andreas , Koltai Gabriella , Zhang Haiwei 
Published: May 2023

AbstractDamage to speleothems is a common phenomenon in mid-latitude caves, and multiple causes have been proposed. Here we report on one of such type of damage, namely stalagmites that are broken and partially sheared near their base but are still in upright position. Such stalagmites occur in the Obir Caves (Austria) associated with cryogenic cave carbonates, demonstrating the former presence of cave ice. 230Th dating suggests damage to the speleothems during the Last Glacial Maximum. Numerical modelling combined with laboratory measurements demonstrates that internal deformation within a cave ice body cannot fracture stalagmites, even on a steep slope. Instead, temperature changes lead to thermoelastic stresses within an ice body that reach values equaling to and exceeding the tensile strength of even large stalagmites. Differences in thermal expansion coefficients cause a sharp vertical jump in stress between the stalagmite and the surrounding ice body, and the ice lifts the stalagmite as it expands with increasing temperature. This study refutes the previously accepted model that flow of ice breaks stalagmites, and suggests a link between glacial climate variability and corresponding cooling and warming cycles in the subsurface that weaken and eventually fracture stalagmites due to the opposing thermoelastic properties of calcite and ice.
Detection of long terminal repeat loci derived from endogenous retrovirus in junglefowl using whole-genome sequencing
Authors: Ishihara Shinya 
Published: May 2023

AbstractEndogenous retroviruses (ERVs) are genetic elements present in the genome that retain traces of past viral infections. Characterization of ERVs can provide crucial insights into avian evolution. This study aimed to identify novel long terminal repeat (LTR) loci derived from ERVs (ERV-LTRs) absent in the reference genome using whole-genome sequencing data of red junglefowl, gray junglefowl, Ceylon junglefowl, and green junglefowl. In total, 835 ERV-LTR loci were identified across the four Gallus species. The numbers of ERV-LTRs loci detected in red junglefowl and its subspecies gray junglefowl, Ceylon junglefowl, and green junglefowl were 362, 216, 193, and 128, respectively. The phylogenetic tree was congruent with previously reported trees, suggesting the potential for inferring relationships among past junglefowl populations from the identified ERV-LTR loci. Of the detected loci, 306 ERV-LTRs were identified near or within the genes, and some were associated with cell adhesion. The detected ERV-LTR sequences were classified as endogenous avian retrovirus family, avian leukosis virus subgroup E, Ovex-1, and murine leukemia virus-related ERVs. In addition, the sequence of the EAV family was divided into four patterns by combining the U3, R, and U5 regions. These findings contribute to a more comprehensive understanding of the characteristics of junglefowl ERVs.
High Curie temperature ferromagnetic structures of (Sb2Te3)1−x(MnSb2Te4)x with x = 0.7–0.8
Authors: Levy Ido , Forrester Candice , Ding Xiaxin , Testelin Christophe , Krusin-Elbaum Lia , Tamargo Maria C. 
Published: May 2023

AbstractMagnetic topological materials are promising for realizing novel quantum physical phenomena. Among these, bulk Mn-rich MnSb2Te4is ferromagnetic due to MnSbantisites and has relatively high Curie temperatures (TC), which is attractive for technological applications. We have previously reported the growth of materials with the formula (Sb2Te3)1−x(MnSb2Te4)x, where x varies between 0 and 1. Here we report on their magnetic and transport properties. We show that the samples are divided into three groups based on the value of x (or the percent septuple layers within the crystals) and their corresponding TCvalues. Samples that contain x < 0.7 or x > 0.9 have a single TCvalue of 15–20 K and 20–30 K, respectively, while samples with 0.7 < x < 0.8 exhibit two TCvalues, one (TC1) at ~ 25 K and the second (TC2) reaching values above 80 K, almost twice as high as any reported value to date for these types of materials. Structural analysis shows that samples with 0.7 < x < 0.8 have large regions of only SLs, while other regions have isolated QLs embedded within the SL lattice. We propose that the SL regions give rise to a TC1of ~ 20 to 30 K, and regions with isolated QLs are responsible for the higher TC2values. Our results have important implications for the design of magnetic topological materials having enhanced properties.
Optimized metrics for orthogonal combinatorial CRISPR screens
Authors: Cetin Ronay , Wegner Martin , Luwisch Leah , Saud Sarada , Achmedov Tatjana , Süsser Sebastian , Vera-Guapi Antonella , Müller Konstantin , Matthess Yves , Quandt Eva , Schaubeck Simone , Beisel Chase L. , Kaulich Manuel 
Published: May 2023

AbstractCRISPR-based gene perturbation enables unbiased investigations of single and combinatorial genotype-to-phenotype associations. In light of efforts to map combinatorial gene dependencies at scale, choosing an efficient and robust CRISPR-associated (Cas) nuclease is of utmost importance. Even though SpCas9 and AsCas12a are widely used for single, combinatorial, and orthogonal screenings, side-by-side comparisons remain sparse. Here, we systematically compared combinatorial SpCas9, AsCas12a, and CHyMErA in hTERT-immortalized retinal pigment epithelial cells and extracted performance-critical parameters for combinatorial and orthogonal CRISPR screens. Our analyses identified SpCas9 to be superior to enhanced and optimized AsCas12a, with CHyMErA being largely inactive in the tested conditions. Since AsCas12a contains RNA processing activity, we used arrayed dual-gRNAs to improve AsCas12a and CHyMErA applications. While this negatively influenced the effect size range of combinatorial AsCas12a applications, it enhanced the performance of CHyMErA. This improved performance, however, was limited to AsCas12a dual-gRNAs, as SpCas9 gRNAs remained largely inactive. To avoid the use of hybrid gRNAs for orthogonal applications, we engineered the multiplex SpCas9-enAsCas12a approach (multiSPAS) that avoids RNA processing for efficient orthogonal gene editing.
Orai, RyR, and IP3R channels cooperatively regulate calcium signaling in brain mid-capillary pericytes
Authors: Phillips Braxton , Clark Jenna , Martineau Éric , Rungta Ravi L. 
Published: May 2023

AbstractPericytes are multifunctional cells of the vasculature that are vital to brain homeostasis, yet many of their fundamental physiological properties, such as Ca2+ signaling pathways, remain unexplored. We performed pharmacological and ion substitution experiments to investigate the mechanisms underlying pericyte Ca2+ signaling in acute cortical brain slices of PDGFRβ-Cre::GCaMP6f mice. We report that mid-capillary pericyte Ca2+ signalling differs from ensheathing type pericytes in that it is largely independent of L- and T-type voltage-gated calcium channels. Instead, Ca2+ signals in mid-capillary pericytes were inhibited by multiple Orai channel blockers, which also inhibited Ca2+ entry triggered by endoplasmic reticulum (ER) store depletion. An investigation into store release pathways indicated that Ca2+ transients in mid-capillary pericytes occur through a combination of IP3R and RyR activation, and that Orai store-operated calcium entry (SOCE) is required to sustain and amplify intracellular Ca2+ increases evoked by the GqGPCR agonist endothelin-1. These results suggest that Ca2+ influx via Orai channels reciprocally regulates IP3R and RyR release pathways in the ER, which together generate spontaneous Ca2+ transients and amplify Gq-coupled Ca2+ elevations in mid-capillary pericytes. Thus, SOCE is a major regulator of pericyte Ca2+ and a target for manipulating their function in health and disease.
OAF is a DAF-like gene that controls ovule development in plants
Authors: Li Ya-Chun , Lin Jhe-Yi , Hsu Wei-Han , Kung Chen-Ting , Dai Shu-Yu , Yang Jun-Yi , Tan Choon-Meng , Yang Chang-Hsien 
Published: May 2023

AbstractWe previously found that the RING-type E3 ligase DEFECTIVE IN ANTHER DEHISCENCE1- (DAD1-) Activating Factor (DAF) controls anther dehiscence by activating the jasmonate biosynthetic pathway in Arabidopsis. Here, we show that in Arabidopsis, the DAF ancestor was duplicated into three genes (DAF, Ovule Activating Factor (OAF), DAFL2), which evolved divergent partial functions from their ancestor through subfunctionalization. In this case, DAF-DAD1-JA signaling regulates anther dehiscence, whereas OAF controls ovule development by negatively regulating cinnamyl alcohol dehydrogenase 9 (CAD9) activity and being negatively regulated by miR847 itself in Arabidopsis. Downregulation of OAF or upregulation of CAD9 and miR847 caused similar abortion of ovule formation due to precocious ovule lignification in transgenic Arabidopsis. Interestingly, only one DAF-like gene, PaOAF, exists in the monocot orchids, which has likely evolved through nonfunctionalization and maintains a conserved function as Arabidopsis OAF in regulating ovule development since defective ovules were observed in the virus-induced gene silencing (VIGS) PaOAF Phalaenopsis orchids. The absence of the DAF ortholog and its function in orchids is likely due to the evolution of stamens to a unique pollinium structure that lacks the feature of anther dehiscence. These findings expand the current knowledge underlying the multifunctional evolution and diverse functionalization of duplicate gene pairs within/among plants.
Design of multi‐phase permanent magnet synchronous motor with fractional‐slot windings for electromagnetic vibration reduction considering stator teeth modulation
Authors: Liu Zhiguang , Yang Zhiyong , Wang Xiaoyuan , Yu Pengcheng , Li Zhiqiang 
Published: May 2023

AbstractA multi‐phase permanent magnet synchronous motor (PMSM) with fractional‐slot windings is proposed to reduce electromagnetic vibration and improve torque performance. Firstly, the analytical expression of electromagnetic force is derived, and the stator teeth modulation effect of electromagnetic force is introduced. The spatial order of the electromagnetic force and the stator teeth modulation are closely related to the slot‐pole combination of the PMSM. Then, three PMSMs with different slot‐pole combinations are presented, and they represent fractional‐slot concentrated winding, integral‐slot distributed winding and fractional‐slot distributed winding, respectively. Their torque performance and electromagnetic vibration performance are studied and compared considering stator teeth modulation. In addition, the multi‐phase winding technology is adopted to further improve the torque performance of the proposed PMSM. Finally, a 90s20p PMSM is manufactured, and the finite element method and experimental method are used to verify the proposed low vibration design method.
Static voltage stability margin prediction considering new energy uncertainty based on graph attention networks and long short‐term memory networks
Authors: Liu Tong , Gu Xueping , Li Shaoyan , Bai Yansong , Wang Tieqiang , Yang Xiaodong 
Published: May 2023

AbstractThe existing static voltage stability margin evaluation methods cannot meet the actual demand of current power grid well in terms of calculation speed and accuracy. Thus, this paper proposes a static voltage stability margin prediction method based on a graph attention network (GAT) and a long short‐term memory network (LSTM) to predict the static voltage stability margin of a power system accurately, fast, and effectively, considering new energy uncertainty. First, an innovative machine learning framework named the GAT‐LSTM is designed to extract highly representative power grid operation features considering the spatial‐temporal correlation of the power grid operation. Then, a static voltage stability margin prediction method based on the GAT‐LSTM is developed. Particularly, considering the influence of new energy power uncertainty, two loss functions of certainty and uncertainty are used in the proposed method to predict the voltage stability margin and voltage fluctuation range. Finally, the IEEE39‐bus power system and a practical power system are employed to verify the proposed method. The results show that the computational speed of the proposed method is greatly improved compared to the traditional methods not based on machine learning; the computation results are more accurate and reliable than the existing machine learning methods. Compared with the existing methods, the proposed method has higher scalability and applicability.
Electrochemical/I– Dual-Catalyzed Access to Sulfonated Pyrazoles under External Oxidant-Free Conditions
Authors: Ma Jing , Yang Jianjing , Wen Jiangwei , Yan Kelu , Luo Boju , Huang Kexin , Wu Ziling , Zhou Yumeng , Zhu Shuyun , Zhao Xian-En 
Published: May 2023

AbstractAn electrochemical/I– dual-catalyzed access to sulfonated pyrazoles from pyrazolones and sodium sulfites under external oxidant-free conditions has been developed. This established electrochemical reaction works smoothly under external oxidant-free conditions and has the advantages of good functional group tolerance, easy to gram-scale synthesis, delivering up to 95% yield for 35 examples.
Computed Tomography–Guided Spinal Biopsy in Suspected Infective Spondylodiscitis: An Institutional Review of Its Utility
Authors: Ravichandran Raj Chendur Abhishek , Amritanand Rohit , Moses Vinu , Kandagaddala Madhavi , Krishnan Venkatesh , David Kenny Samuel , Arockiaraj Justin , Kulasekaran Harini , Ganesan Maya Pavalasseril , Prabhu Anne Jennifer , Keshava Shyamkumar Nidugala 
Published: May 2023

Abstract
Background Infectious spondylodiscitis is a debilitating condition and evidence-based medicine dictates confirming the diagnosis before treatment. Computed tomography–guided spinal biopsy plays a major role and hence we would like to determine its utility in current clinical practice.
Purpose The purpose of this study is to determine the percentage of confirmatory positives of CT-guided spinal biopsy in patients who were clinicoradiologically diagnosed with infectious spondylitis.
Material and Methods A retrospective analysis of patients who underwent CT-guided biopsy for suspected infectious spondylodiscitis from 2017 to 2021 in a tertiary medical center was done. The data were filtered and obtained from the electronic database of the institution.
Results In all, 259 patients underwent CT-guided biopsy of the spine. The procedure provided confirmatory results in 149 (57.5%) biospecimens. Histopathology examination was confirmatory in 95 (36.6%) of the 241 biospecimens sent. The Mycobacteria Growth Indicator Tube (MGIT) was confirmatory in 51 (19.9%) of the 250 biospecimens sent and drug resistance was seen in 6/51 (11.7%) biospecimens. Xpert TB provided confirmatory results in 72 (27.8%) of the 254 biospecimens sent and rifampicin resistance was seen in 16/72 (22.2%) biospecimens. Bacterial culture was confirmatory in 29 (11.2%) of the 250 biospecimens sent. The complication documented in this study was 0.3%.
Conclusion CT-guided spinal biopsy for suspected vertebral osteomyelitis is a safe and effective minimally invasive procedure. It demonstrates a positive yield in more than half of the patients. Knowing the outcome, the patients can be appropriately counseled prior to the procedure. CT-guided biopsy results were affected by prior administration of ATT (antitubercular therapy) in suspected tuberculous spondylitis patients.