bluuebunny/crossref_metadata_2025_split huggingface dataset:
Polyaniline Sandwiched Ultra-strong PVA/PAA Hybrid Hydrogel for Low-temperature Resistant Supercapacitors and Strain Sensors
Authors: Xu Chang , Guan Shiqiang , Dong Xufeng , Huang Hao , Qi Min 
Published: January 2023

Abstract
Hydrogels are a class of promising candidate materials for the preparation of emerging quasi-solid-state supercapacitor electrolytes. However, the inherent defects of poor mechanical strength, easy fragmentation, and bad low-temperature tolerance seriously hinder their further practical application. Herein, a polyaniline sandwiched polyvinyl alcohol/polyacrylic acid hybrid hydrogel (PVA-PAA-PANI) electrolyte with high strength and ideal low-temperature resistance was constructed by combining the Hofmeister effect and in-situ polymerization. This PVA-PAA-PANI hydrogel can provide amazing comprehensive mechanical properties, including 3.5 MPa of breaking strength, 700% of elongation at break, and 11.5 MJ/m3 of toughness, and exhibits satisfactory fatigue resistance. This PVA-PAA-PANI hydrogel-based supercapacitor has a specific capacitance of 32.4 mF/cm2 and achieves 61% capacitance retention even under the harsh cold environment of -20°C. Notably, the capacitance retention reaches 79.2% of the initial state after suffering more than 5000 continuous charge/discharge cycles at -20°C. This PVA-PAA-PANI hydrogel also has good linear sensing behavior and can accurately detect different limb movements and even breathing or speech, demonstrating ideal strain sensing properties. This work develops new strategies for the preparation of high-strength hydrogels for energy storage devices and sensors, and promotes their application in real-life applications.
CDC42—a promising target of glioma treatment related to Treg cell proliferation
Authors: Jiang Tao , Wang Xianwei , Huang Jiaming , Chen Dong 
Published: January 2023

Abstract
Glioma is the worst prognostic neoplasm in the central nervous system. A polarity-regulating GTPase in cells, which is called CdC42, has been proven that its overactivation is tightly connected to the high malignancy of tumor. The RNA-seq and protein expression of CDC42 in tumor and comparison tissues were analyzed based on the online tools, CDC42 is remarkably boosted in tumor tissue compared to controls. 600 patients in the analysis set from the TCGA database and 654 patients in the validation set from the Chinese Glioma Genome Atlas (CGGA) database were adopted. The expression of CDC42 in various clinicopathological features was analyzed, including differential expression, survival analysis, GO and KEGG analysis, immune infiltration, correlated signaling pathway. It was found that CDC42 could be a potential biomarker of glioma transcriptional subtyping. The enrichment of CDC42 was shown to be an independent indicator of poor prognosis for glioma by Cox analysis and KM curves.Additionally, the concentration extent of CDC42 was closely related to immune infiltration, immune checkpoint inhibitors, and Treg cell markers (CD4, CD25, CD127). Further GSEA analysis demonstrated that CDC42 was significantly connected with the differentiation, migration and proliferation of T regulatory (Treg) cell through the PI3K/AKT signaling pathway.
Construction of the first ultra-dense genetic map for faba bean (Vicia faba L.) and quantitative trait loci mapping for seed-related traits
Authors: Zhao Na , Xue Dong , Miao Yamei , Wang Xuejun , Wang Yongqiang , Zhou Yao , Zhou Enqiang , Yao Mengnan , Gu Chunyan , Wang Kaihua , Li Bo , Wei Libin 
Published: January 2023

Abstract
Faba bean (Vicia faba L.) is a valuable legume crop and data on its seed-related traits is required for yield and quality improvements. However, basic research on faba bean is lagging compared to that of other major crops. In this study, an F2 faba bean population, including 121 plants derived from a WY7 and TCX7 cross, was genotyped using the Faba_bean_130 K targeted next-generation sequencing genotyping platform. The data were used to construct the first ultra-dense faba bean genetic map consisting of 12,023 single nucleotide polymorphisms markers covering 1182.65 cM with an average distance of 0.098 cM. The map consisted of 6 linkage groups, which is consistent with the 6 faba bean chromosome pairs. A total of 65 quantitative trait loci (QTL) for seed-related traits were identified (3 for 100-seed weight, 28 for seed shape, 12 for seed coat color, and 22 for nutritional quality). Furthermore, 333 candidate genes that are likely to participate in the regulation of seed-related traits were also identified. Our research and its findings can provide a basis for future faba bean marker-assisted breeding and reference genome assembly.
Granular cell tumor of the hypopharynx: a rare case report.
Authors: Oulghoul Omar , Rizkou FatimaEzzahra , BENHOUMMAD OTHMANE , ROCHDI YOUSSEF , RAJI ADBELAZIZ 
Published: January 2023

Abstract
Introduction: Originating from the Schwann cells; Granular cell tumor is an extremely rare tumor, accounting for 0.5% of all soft tissue tumors. Variant of this tumor can be classified as either as synchronous or metachronous, or either malignant, begin or atypical. We report a scarce case of granular cell tumor of the hypopharynx, adding to the rare count of this entity in this localization, in a 74 years-old female patient.
Case presentation : We add to the literature arsenal of this entity by reporting this rare case of benign of granular cell tumor of the hypopharynx in A 74 years-old female patient with hypertension, suffered from dysphonia for about 6 months. Examination identified a budding tumor in the left piriform sinus. Ct scan showcased a 1 cm irregular, spontaneously hypodense, slightly enhanced after injection mass, infiltrating the left piriform sinus. Excisional biopsy confirmed the diagnosis and follow-up was uneventful.
Conclusion: This rare neoplasia have variable age, sex and ethnicity predilection; and depending on the localization, nonspecific symptoms and it is usually displays as small, smooth, submucosal lesion. Positive staining for S-100 protein confirms the diagnosis and no harmonized treatment guideline is established.
C-Terminal Domain Phosphatase-Like 1 Protein Promotes Flowering With TAF15b by Repressing the Floral Repressor Gene Flowering Locus C
Authors: Kyung Jinseul , Jeong Daesong , Eom Hyunjoo , Kim Jeesoo , Kim Jong-Seo , Lee Ilha 
Published: January 2023

Abstract
Arabidopsis TBP-ASSOCIATED FACTOR15b (TAF15b) is a plant-specific component of the transcription factor IID (TFIID) complex. TAF15b is involved in the autonomous pathway for flowering and represses transcription of FLOWERING LOCUS C (FLC), a strong floral repressor in Arabidopsis. Although the components of the autonomous pathway for flowering have been studied extensively, little is known about the functional partners of TAF15b and the mechanism of how TAF15b works. Here, we show that C-TERMINAL DOMAIN PHOSPHATASE-LIKE 1 (CPL1) is a physical and functional partner of TAF15b for the transcriptional repression of FLC. CPL1 is a protein phosphatase that dephosphorylates C-terminal domain (CTD) of RNA polymerase II (Pol II). Through the immunoprecipitation and mass spectrometry technique, we fished out the CPL1 protein as an interacting partner of TAF15b. Similar to taf15b, the cpl1 mutant showed a late-flowering phenotype caused by the increase of FLC expression. In addition, FLC increase in cpl1 was correlated with the enrichment of phosphorylated Pol II in FLC chromatin as expected. We also found that CPL1 and TAF15b share additional common target genes through transcriptome analysis. Such results suggest that the TAF15b–CPL1 complex directly represses transcription through dephosphorylation of Pol II, especially at the FLC locus.
Combined effects of freezing-thawing cycles and livestock excreta deposition complicate soil nitrogen and phosphorus nutrient dynamics in an alpine steppe of northern Tibetan Plateau, China
Authors: Du Ziyin , Zhang Qingsong , Sun Jian , Hong Jiangtao , Xu Xin , Pang Bo , Li Jiaxiu , Wang Xiaodan 
Published: January 2023

Abstract
Background and Aims: Livestock excreta (dung and urine) is one of the significant sources of soil nitrogen (N) and phosphorus (P) nutrients in grazed grasslands, and freezing-thawing cycle (FTC) during non-growing season are considered to complicatedly affect soil N and P dynamics by changing excreta nutrients release, soil physicochemical, and microbial properties. However, the combined effects and functional mechanisms of FTCs and excreta deposition on the availability, transformation, and migration processes of soil N and P nutrients in alpine grasslands need further clarifications.
Methods: A 277-day experiment was conducted to investigate the response of dung N and P nutrients release, soil available N and P dynamics, and N transformation process to FTCs at different freezing-thawing periods, including treatments of control (CK), soils respectively treated with yak dung (YD), Tibetan sheep dung (TSD), yak urine (YU), and Tibetan sheep urine (TSU) in an alpine steppe of northern Tibetan Plateau.
Results: Enhanced mineralization during the stable freezing period significantly (P < 0.05) increased alpine steppe soil ammonium-N (NH4+-N) and available P (AP) concentrations at 0–5 cm depth. The significant (P < 0.05) increase in soil nitrate-N (NO3--N) concentration at 0−5 and 5−10 cm depths during the thawing period was a response to the improvement in nitrification. The YU and TSU deposition respectively resulted in the 0−5 cm soil NH4+-N concentration reached 127 and 89.4 mg/kg and the corresponding NO3--N concentration reached 90.5 and 29.6 mg/kg, with these peaked values occurred during freezing period significantly (P < 0.05) greater than other freezing–thawing periods. Interactions between excreta treatment and freezing–thawing periods significantly (P < 0.001) affected soil NH4+-N and NO3--N concentrations, and soil AP variation was strongly (P < 0.001) correlated with freezing–thawing periods. Trade-off between NH4+-N and NO3--N was closely related to the influence of nitrification and denitrification, which were affected by frequent FTCs during the winter freezing and spring thawing.
Conclusion: Increases in mineral N and AP concentrations during the spring thawing period likely altered the phenological properties of vegetation and the productivity of grassland ecosystems to varying degrees. Our study would provide scientific guidance for optimizing the management mode of livestock excreta during freezing–thawing periods and promoting the sustainable development of alpine grassland ecosystems.
Novel anoikis-related diagnostic biomarkers for aortic dissection based on machine learning
Authors: Zhang Hanyi , Dong Xiangling , Ouyang Zhen , Zhou Tianji , Chen Jia , Wang Mi 
Published: January 2023

Abstract
Aortic dissection (AD) is one of the most dangerous diseases of the cardiovascular system, which is characterized by acute onset and poor prognosis, while the pathogenesis of AD is still unclear and may affect or even delay the diagnosis of AD. Anchorage-dependent cell death (Anoikis) is a special mode of cell death, which is programmed cell death caused by normal cells after detachment from extracellular matrix (ECM)and has been widely studied in the field of oncology in recent years. In this study, we applied bioinformatics analysis, according to the results of research analysis and Gene Ontology (GO), as well as Kyoto Encyclopedia of Genes and Genomes (KEGG), finally found 3 anoikis-related genes (ARGs) based on machine learning. Then we further verified by receiver operating characteristic (ROC), gene set enrichment analysis (GSEA), gene set enrichment analysis (GSVA)and other methods. We hypothesize ARGs may be involved in the pathogenesis of AD through pathways such as oxidative stress, inflammatory response, and ECM. Therefore, we conclude that the ARGs can be an important factor in determining the diagnosis of AD.
Sustainable transhumance practices in European landscapes. A comparative study
Authors: Pablo Vidal-González , Gylfi Helgason , Daníel Júlíusson Árni 
Published: January 2023

Abstract
Transhumant practices are a clear and diachronic example of sustainable use of the available pasture land, taking advantage of climatic and environmental differences between summer and winter pastures. This more balanced use of natural resources has an ancient history and made it possible for local communities in rural societies to exploit areas that would otherwise not have been used for agricultural purposes. Industrialisation, globalisation, and the collapse of rural economy in the 20th century led to a profound transformation of these traditional pastoral practices. In this paper, we present a cursory look on transhumance from Northern Iceland and Eastern Spain to give us an insight regarding how different communities strived towards sustainable livestock practice and landscape management in the past. Traditional pastoralism aims to use balancing strategies to prevent the degradation of land and the environment. The implementation of seasonal schedules of pasture areas to avoid overgrazing, implementing control regarding the utilisation of the grazing areas, and setting disputes between different social groups that have access to the pasture lands are great examples of social strategies maintained by transhumance communities to promote sustainable landscape management. This paper will examine archaeological and ethnoarchaeological evidence to enable the comparison between the two selected case studies. We believe that the diversity and complex history of these case studies can offer us a valuable lesson of good practice for a sustainable and environmentally friendly use of European pastoral territories in present-day societies.
Study Protocol Supporting family caregivers of people with dementia and identifying an effective adaptive intervention to reduce their depressive symptoms: protocol of a sequential multiple assignment randomized trial
Authors: Kor Patrick Pui Kin , Chou Kee Lee , Zarit Steven Howard , Gallagher-Thompson Dolores , Galante Julieta , Wong Samuel Yeung Shan , Cheung Daphne Sze Ki , Leung Angela Yee Man , Chu Leung Wing 
Published: January 2023

Abstract
Background: Family caregivers of people with dementia (FG-of-PWD) suffer from a high level of stress and depressive symptoms, which usually require different interventions at different stages. Although some standalone interventions such as behavioral activation (BA) and mindfulness practice (MP) have been shown to be potentially effective at reducing depressive symptoms, the best sequence and combination of these interventions for caregivers are unknown. This study aims to develop and identify a two-stage adaptive intervention with pre-specified rules guiding whether, how, or when to offer different interventions initially/over time to reduce depressive symptoms in FG-of-PWD.
Methods: A sequential multiple assignment randomized trial (SMART) design will be adopted. 272 FG-of-PWD with mild to moderate depressive symptoms will be recruited from the community. Four two-stage embedded adaptive interventions involving behavioral activation and mindfulness practice with different sequences and dosages (e.g., 8 weeks of Behavioral Activation followed by booster sessions for responders, and 8 weeks of mindfulness practice for non-responders) will be assigned to the participants following a set of decision rules. The primary outcomes will be depressive symptoms, assessed after the second stage of the intervention. Other outcomes (e.g., positive aspect of caregiving and sleep quality) and time-points will also be assessed. The analyses will follow the intention-to-treat principle. In addition, several process indicators (e.g. engagement in meaningful activities and level of mindfulness) will also be assessed.
Discussion: The traditional RCT design allows for the effectiveness of an intervention to be rigorously tested but provides limited data to develop “treatment strategies” that may involve a number of different interventions, dosages, or components. We propose using the SMART design to provide evidence of which intervention to provide initially, how to determine whether the first stage of the intervention is working or not, and which intervention to provide next if the first stage of the intervention is not working. The findings will have strong implications for the further development of psycho-social adaptive intervention in reducing the depressive symptoms among FC-of-PWD.
Trial registration: ClinicalTrials.gov, NCT05634317
Effect of the addition ratio of rice flour and molasses on growth performance and survival of Pacific white shrimp (Litopenaeus vannamei) stocked by biofloc technology
Authors: Phuong Ta Van , Hoa Nguyen Van , Vo Van-Thanh , Diep Doan Xuan 
Published: January 2023

Abstract
The 63-day experiment was conducted to investigate the effect of different rice flour and molasses ratio on Pacific white shrimp, which were reared by biofloc technology. The five treatments (R10-M90, R30-M70, R50-M50, R30-M70, R10-M90) and one control were randomly arranged with three repetitions in 18 plastic tanks. The content of adding two carbohydrate sources was calculated and added to water to gain the C:N ratio of 15:1. The experimental shrimps (mean initial weight of 0.095 g) were stocked at a density of 150 m−3 and fed Lotus pellets (40% protein) for 63 days. The results showed that the temperature, pH, DO, and alkalinity are suitable for shrimp. The carbohydrate-supplemented treatments increased floc volume (FVI), mean floc length (MFL) and mean floc width (MFW), total suspended solids (TSS), reduced total ammonia nitrogen (TAN), nitrite (NO2-), as compared to control, in which R70-M30 treatment exhibited the most pronounced effects. Shrimp culture applying biofloc process stimulated the density of total bacteria and zooplankton to thrive and limited phytoplankton. Maintaining the C:N as 15 by adding carbohydrates showed a more effective impact. There was no significant difference in final average weight, but final biomass and survival tended to be highest in treatment R70-M30 (results were only significantly different from R90-M10). In conclusion, combining two carbohydrate sources positively improved water quality and had no negative impact on shrimp growth.
Informed consent an illusion or legal litigation? Perceptions of today’s doctors.
Authors: mulla humeera , mulla huzaifa 
Published: January 2023

Abstract
Introduction: Informed consent is a foundation of modern medical practice. It is a process that bridges the gap and leads to the interaction between the health care provider and the patient. One of the four key principles of biomedical ethics outlined by Beauchamp and Childress works to defend patients' autonomy.1 An ethical and legal need is to obtain a proper informed consent. The doctor has a moral obligation to uphold patients' human dignity while seeking informed consent.3 Aim: To evaluate the perception of doctors towards informed consent in a quaternary care hospital. Material and method: A questionnaire was prepared and distributed among the doctors personally. Results: Consultants and senior residents of both clinical and nonclinical departments have adequate awareness and knowledge of medical ethics. Conclusions: Any medical professional must prioritise patient satisfaction. It is largely influenced by informed consent, which is a medico-legal contributing factor. In order to bridge the gap between professionals and their knowledge, awareness, and ethical practises, efforts should be made to sensitise them. Possible interventions include conferences, symposiums, and workshops on health care ethics.
French translation and validation of the Neck Dissection Impairment Index, a quality of life measure for the surgical oncology population
Authors: Khoury Michel , Guertin William , Hao Cameo , Saltychev Mikhail , Ayad Tareck , Bissada Eric , Christopoulos Apostolos , Moubayed Sami , Olivier Marie-Jo , Chepeha Douglas , Lai Stephen Y. , Maniakas Anastasios 
Published: January 2023

Abstract
Background: Neck dissections (ND) are a routine procedure in head and neck oncology. Given the post-operative functional impact that some patients experience, it is imperative to identify and track quality of life (QoL) symptomatology in order to tailor each patient’s therapeutic needs. To date, there is no validated francophone questionnaire for this patient-population. We therefore sought to translate and validate the Neck Dissection Impairment Index (NDII) in French. Methods: A three-phased approach was used. Phase 1: The NDII was translated from English to French using a “forward and backward” translational technique following international guidelines. Phase 2: A cognitive debriefing session was held with ten French-speaking otolaryngology patients to evaluate understandability and acceptability. Phase 3: The final version was administered prospectively to 30 patients with prior history of ND and 30 control patients. These patients were asked to complete the questionnaire 2 weeks after their first response. Test-retest reliability was calculated with Spearman’s correlation. Internal consistency was elicited using Cronbach’s alpha.
Results: NDII was successfully translated and validated to French. Cronbach’s alpha revealed high internal consistency (0.92, lower 95% CL 0.89). The correlation for test-retest validity were strong or very-strong (0.61-0.91).
Conclusion: NDII is an internationally recognized QoL tool for the identification of ND-related impairments. This validated French version will allow clinicians to adequately assess the surgery-related QoL effect of neck surgery in the French-speaking population, while allowing French institutions to conduct and/or participate in multi-site clinical trials requiring the NDII as an outcome measure.
Consolidative thoracic radiation could not improve survival of extensive-stage small-cell lung cancer with first-line chemoimmunotherapy: results from a single cancer center
Authors: Li Yuying , Jing Wang , Jing Xuquan , Sun Yulan , Tang Xiaoyong , Guo Jun , Zhang Yan , Zhu Hui 
Published: January 2023

Abstract
Objective
To investigate the role of consolidative thoracic radiation (TRT) in extensive-stage small-cell lung cancer (ES-SCLC) receiving first-line chemo-immunotherapy followed by immunotherapy maintenance.
Patients and Methods:
Outcomes of patients without disease progression after first-line chemotherapy were reviewed (January 2020 to December 2021). Based on TRT or not, patients were allocated to TRT group or non-TRT group. Progression-free survival (PFS), overall survival (OS) and local-recurrence free survival (LRFS) were calculated by the Kaplan-Meier method and compared by log-rank test.
Results
Of 100 patients, 47 received TRT and 53 non-TRT. The median follow-up was 20.3 months. The median PFS and OS in TRT were 9.1 months and 21.8 months, versus 8.8 months (p = 0.93) and 24.3 months (p = 0.63), respectively, in non-TRT. The median LRFS time in TRT was not reached, but significantly longer than 10.8 months in non-TRT (HR = 0.27, p < 0.01). Second-line chemotherapy significantly prolonged survival compared to that with chemo-free patients (mOS: 24.5 vs. 21.4 months, p = 0.026). The subgroup analysis showed a trend of patients with brain metastases benefit from TRT (21.8 versus 13.7 months, HR 0.61, p = 0.38) while liver metastases did not. Of 47 patients with TRT, only 10.6% of patients experienced grade 3 radiation-induced pneumonitis, while no grade 4 or 5 adverse events occurred.
Conclusion
Consolidative TRT in the period of immunotherapy maintenance followed first-line chemo-immunotherapy did not prolong OS and PFS but increased LRFS in ES-SCLC.