bluuebunny/crossref_metadata_2025_split huggingface dataset:
IRIS U kit usefulness in transanal total mesorectal excision for lower rectal cancer to avoid urethral injury
Authors: Ishii Masatsugu , Nitta Toshikatsu , Kataoka Jun , Ueda Yasuhiko , Senpuku Sadakatsu , Matsutani Ayumi , Taki Masataka , Horiguchi Kohei , Ota Masato , Ishibashi Takashi 
Published: August 2023

Abstract

Transanal total mesorectal excision (taTME) has improved the laparoscopic dissection for rectal cancer in the narrow pelvis. Although taTME has more clinical benefits than laparoscopic surgery, such as a better view of the distal rectum and direct determination of distal resection margin, an intraoperative urethral injury could occur in excision ta-TME. This study aimed to determine the feasibility and efficacy of the ta-TME with IRIS U kit surgery. This retrospective study enrolled 10 rectal cancer patients who underwent a taTME with an IRIS U kit. The study endpoints were the safety of access (intra- or postoperative morbidity). The detectability of the IRIS U kit catheter was investigated by using a laparoscope-ICG fluorescence camera system. Their mean age was 71.4±6.4 (58–78) years; 80 were men, and 2 were women. The mean operative time was 534.6 ± 94.5 min. The coloanal anastomosis was performed in 80%, and 20% underwent abdominal peritoneal resection. Two patients encountered postoperative complications graded as Clavien–Dindo grade 2. The transanal approach with IRIS U kit assistance is feasible, safe for patients with lower rectal cancer, and may prevent intraoperative urethral injury.
Ethical dilemmas in COVID-19 patients awaiting lung transplantation on extracorporeal membrane oxygenation
Authors: Trejnowska Ewa , Urlik Maciej , Ochman Marek , Wajda-Pokrontka Marta , Cyprys Paweł , Przybyłowski Piotr , Skoczyński Szymon , Knapik Piotr 
Published: August 2023

Abstract

Background: Extracorporeal membrane oxygenation (ECMO) may serve as a bridge to
successful lung transplantation (LT) in selected patients with coronavirus disease 19 (COVID-
19) pneumonia. Compliance with the recognized LT criteria however, may be impossible to
be met in this particular and extremely challenging group of patients. This situation create
huge moral and ethical dilemmas. To indicate this, we decided to explore this difficult topic
and present how non-compliance to the recognized LT criteria enabled us to perform three
successful and life-saving LT procedures in COVID-19 patients.
Methods: Data of all consecutive patients on ECMO support as rescue treatment for COVID-
19 pneumonia, awaiting LT at SCHD between October 1, 2020 and May 31, 2021 were
reviewed.
Results: 18 patients on ECMO entered the list for LT at SCHD (mean age: 40.5 ± 5.6 years,
range 28–48 years). Mean duration of ECMO was 29.6 ± 15.5 days (range 6–71 days). 11
patients died: either during LT (n = 3), post-LT (n = 1), or on ECMO awaiting LT (n = 7).
The overall mortality rate in this group was 61.1%. Survivors (n=7) were either successfully
transplanted (n = 3) or weaned from ECMO (n = 4). In the medical literature, there are several
enthusiastic case reports describing successful LTs, however there might be a significant
publication bias in this area, as failed procedures probably remain largely under-reported.
There are no data to indicate the right time for LT in post-COVID-19 patients. Experts in this
field require minimum recovery period of 4 weeks to exclude native lung recovery, active
participation in physical rehabilitation and the possibility of providing informed consent to
proceed with LT. All these conditions were impossible to be met in our patients awaiting LT
on ECMO support.
Conclusions: Traditional lung transplantation criteria are difficult to be applied in COVID-19
patients requiring ECMO support due to medical and ethical reasons. Performing LT without
prior consent of patients violates the ethical principles of solid organ transplants, but may be
life-saving in some patients.
Rapidly adsorption of antibiotics in water onto demolded MCM-41 mesoporous materials
Authors: Chen Jie , Yang Yao , Yao Yuanyuan , Huang Zhujian , Xu Qiaolin , He Liping , Gong Beini 
Published: August 2023

Abstract
Antibiotic pollution has seriously affected human life and the ecological environment. And the development of economical and environmentally friendly adsorbent materials has become a research hotspot in this field. Mesoporous molecular sieve MCM-41 has good adsorption capacity, low cost, and strong adsorbent regeneration, with a high application value. Here, we studied the adsorption effects of demolded MCM-41 materials on tetracycline, doxycycline, and levofloxacin at different temperatures. The optimal demolding temperature was determined to be 800°C, and the adsorption experiments on three kinds of pollutants were conducted. The results showed that neutral or weak acid pH was beneficial to the adsorption. Also, alkaline pH inhibited adsorption; therefore, the Langmuir model could fit the adsorption thermodynamics well. The adsorption of MCM-41 for three kinds of pollutants conformed to the second-order adsorption kinetics model and belonged to chemical adsorption. The results demonstrated that MCM-41 has excellent potential for decontaminating of antibiotics wastewater.
Investigation of serious crimes using DNA from dust and air
Authors: Fantinato Chiara , Fonneløp Ane Elida , Bleka Øyvind , Vigeland Magnus Dehli , Gill Peter 
Published: August 2023

Abstract
Humans constantly shed DNA into the surrounding environment. This DNA may either remain suspended in the air or it settles onto surfaces as house dust.
In this study, we explored the potential use of human DNA recovered from air and dust to investigate crimes where there are no visible traces available – for example, from a recently vacated drugs factory where multiple workers had been present. Samples were collected from three indoor locations (offices, meeting rooms and laboratories) characterized by different occupancy types and cleaning regimes. The resultant DNA profiles were compared with the reference profiles of 55 occupants of the premises. Our findings showed that household dust samples are rich sources of DNA and provide an historical record of occupants within the specific locality of collection. Detectable levels of DNA were also observed in air and dust samples from ultra-clean forensic laboratories which can potentially contaminate casework samples. We provide a Bayesian statistical model to estimate the minimum number of dust samples needed to detect all inhabitants of a location. The results of this study suggest that air and dust could become novel sources of evidence to identify current and past occupants of a crime scene.
Have clinical research and related recommendations been consistent enough for informing the treatment of advanced esophageal cancer patients with anticancer drugs? A critical historical review
Authors: Santero Marilina , Savall-Esteve Olga , Bracchiglione Javier , Requeijo Carolina , Cosp Xavier Bonfill 
Published: August 2023

Abstract
Purpose
to analyse the soundest research and recommendations that have informed the potential superiority of treatments with anticancer drugs over any type of supportive care for advanced esophageal cancer (EC).
Methods
We conducted a critical historical review. First, we identified randomised clinical trials (RCTs) from a previous scoping review performed by our research group ASTAC updating the search strategy. Second, we searched for the most important and recognized international clinical practice guidelines (CPGs) in advanced EC. Finally, we performed a systematic document analysis comparing whether the recommendations proposed in the CPGs were supported by the previously identified relevant evidence.
Results
We identified and assessed 15 randomised clinical trials and 11 clinical practice guidelines from ESMO (eight), ASCO (two) and NICE (one) published in the last 40 years. There is a clear mismatch between these guidelines’ recommendations and the available RCTs in order to inform whether anticancer drugs are more beneficial than BSC.
Conclusion
There is a lack of consistent evidence to treat advanced EC patients as well as a mismatch between the existing evidence and the recommendations made by relevant CPGs. As a consequence, they could have been biased when favouring the use of anticancer drugs over the supportive care.
Between the dog and the wolf – An interpretative phenomenological analysis of bicultural, sexual minority people’s lived experiences
Authors: Miller Emelie Louise , Zakrisson Ingrid 
Published: August 2023

Abstract

Previous research in social psychology has, via intersectional approaches, established that individuals with multiple-minority identities frequently wrestle with psychosocial dilemmas in both ingroup and outgroup settings, which can impede a positive belonging and identity formation. The present study investigates lived experiences held by a multiple-minority group seldom provided sufficient consideration; bicultural, sexual minorities. In-depth interviews were conducted with eight non-heterosexual individuals, with second-generation immigration backgrounds, living in Sweden. Data was analyzed through a collaborative interpretative phenomenological analysis. Two overarching group experiential themes (GETs) were created: “A puzzle of identity struggles” and “Sources of empowerment”. The former included GETs detailing experiences of bullying, misidentification, parental shortcomings, gender issues, and severe mental health challenges. “Sources of empowerment” covered GETs focusing on strengthening factors in the participants’ experiences, which incorporated acts of love, pride in heritage, improved identification with sexuality, creative coping strategies, purpose in life, and positive belonging. Blurred lines existed between general stressors’ and minority-specific stressors’ impact on the participants’ lived experiences, which problematizes simplified understandings of multiple-minorities. Further research on experiences held by ethnic, sexual minority individuals is strongly encouraged, as are advances in multiple-minority knowledge among professionals within the educational, and mental health systems. This would facilitate preventive and interventive work that could improve life conditions and sense of belonging among non-heterosexual, ethnic minorities.
Low Dose Post-transplant Cyclophosphamide and Sirolimus Induce Mixed Chimerism with CTLA4-Ig or Lymphocyte Depletion in an MHC-Mismatched Murine Allotransplantation Model
Authors: Fitzhugh Courtney , Kabore Mariama Djelika , McElrath Corbin , Ali Mohamed , Almengo Katherine , Gangaplara Arun , Fisher Cameron , Barretto Mauricio , Shaikh Ahmad , Olkhanud Purevdorj , Xu Xin , Gaskin Deanna , Saxena Ankit , Lopez-Ocasio Maria , McCoy Phillip 
Published: August 2023

Abstract
Allogeneic hematopoietic stem cell transplantation (allo-HCT) offers a curative option for patients with non-malignant hematological diseases. High-dose post-transplant cyclophosphamide (PT-Cy, 200 mg/kg) and sirolimus (Sir, 3 mg/kg) synergistically induce stable mixed chimerism. Further, Sir and cytotoxic T lymphocyte-associated antigen-4 immunoglobulin (CTLA4-Ig) promote immune tolerance and allograft survival. Here, in a major histocompatibility complex (MHC)-mismatched allo-HCT murine model, we combined CTLA4-Ig and T-cell depleting anti-Thy1.2 with a lower dose of PT-Cy. We demonstrate that low-dose PT-Cy and Sir combined with anti-Thy1.2, with or without CTLA4-Ig, induced similar donor chimerism levels compared to high-dose PT-Cy and Sir. Engrafted groups displayed significantly reduced frequencies of recipient-specific interferon-γ-producing T cells and natural killer (NK) cells. Interestingly, an increased frequency in regulatory T cells (Tregs) was found in engrafted mice except when CTLA4-Ig is combined with low-dose PT-Cy. Splenocytes collected from engrafted mice showed no proliferation upon restimulation with Balb/c stimulators. Collectively, low-dose PT-Cy and Sir combined with Thy1.2 with or without CTLA4-Ig induced mixed chimerism and Treg mediated donor unresponsiveness and reduced inflammatory T cells and B cells. In combination with CTLA4-Ig or lymphocyte depletion, low-dose PT-Cy and Sir may be considered in future regimens to reduce graft rejection in patients who undergo allo-HCT.
New Insights into RPE-Photoreceptor Complex Ultrastructure using Focused Ion Beam-Scanning Election Microscopy (FIB-SEM)
Authors: Low Shermaine W.Y. , Lim Rayne R. , Grant DeAna G. , Patterson Sam , Chaurasia Shyam S. 
Published: August 2023

Abstract
Photoreceptors in the retina are specialized neuronal cells that perceive light and play a central role in the visual system. Damage to photoreceptors is a clinical feature often associated with various retinal degenerative disorders. The photoreceptor bed comprises a unique extracellular matrix (ECM) scaffold often described as the interphotoreceptor matrix (IPM) in the subretinal space, vital during retinal development and homeostasis. In this study, we used focused ion beam scanning electron microscopy (FIB-SEM) and transmission electron microscopy (TEM) to analyze the ultrastructural architecture of the retinal pigmented epithelium (RPE)-photoreceptor complex in mice. Additionally, we describe methods for retinal preparation in EM imaging. TEM images display ultrastructural retina layers, including Bruch’s membrane and the interdigitation zone (IZ). The 3-dimensional reconstruction of the outer retina revealed individual photoreceptors, the connection between their inner and outer segment via the photoreceptor cilia, and photoreceptor interaction with the RPE ciliary processes. Our findings highlight the importance of FIB-SEM in deciphering the ultrastructural details of RPE-photoreceptor interactions in the IPM complex which are essential for the maintenance of retinal architecture.
Predictors of the therapeutic effect of Nab-paclitaxel plus gemcitabine therapy for patients with recurrent pancreatic cancer after pancreatectomy
Authors: Kawaida Hiromichi , Hosomura Naohiro , Amemiya Hidetake , Saito Ryo , Shoda Katsutoshi , Furuya Shinji , Akaike Hidenori , Kawaguchi Yoshihiko , Kono Hiroshi , Fukasawa Yoshimitsu , Kawakami Satoshi , Shindo Hiroko , Takahashi Ei , Takano Shinichi , Fukasawa Mitsuharu , Simizu Tatsuya , Itakura Jun , Enomoto Nobuyuki , Fujii Hideki , Ichikawa Daisuke 
Published: August 2023

Abstract
Background/Aim
Nab-paclitaxel plus gemcitabine (GnP) is one of the standard chemotherapeutic schemes of treatment of advanced pancreatic cancer (PC). The current study has the aim to outline the predictors of therapeutic effect of GnP in the cases with recurrent PC after pancreatectomy.
Patients and Methods
We retrospectively evaluated the clinicopathological characteristics of patients with recurrent PC after pancreatectomy who received GnP at our institution from January 2016 to February 2021. We examined various inflammatory markers [neutrophil lymphocyte ratio (NLR), lymphocyte monocyte ratio (LMR), platelet lymphocyte ratio (PLR), C-reactive protein albumin ratio (CAR)] and a nutritional marker [ prognostic nutritional index (PNI) ] in addition to common clinicopathological characteristics as possible predictors of the chemotherapeutic effects of GnP.
Results
Univariate analysis identified PLR, CAR, and histological differentiation as significant predictors of better chemotherapeutic effects. Meanwhile, multivariate analysis identified only histological differentiation as an independent predictor of better chemotherapeutic effects. Accordingly, patients with well-differentiated adenocarcinoma showed better overall survival after recurrence than those with other histological types.
Conclusion
Histological differentiation can be a useful predictor of the therapeutic effects of GnP in the clinical cases of recurrent PC after pancreatectomy.
Abnormal Centriolar Biomarker Ratios Correlate with Unexplained Bull Artificial Insemination Subfertility – a Pilot Study
Authors: Turner Katerina A. , Achinger Luke , Kong Dong , Kluczynski Derek F. , Fishman Emily Lillian , Phillips Audrey , Saltzman Barbara , Loncarek Jadranka , Harstine Bo R. , Avidor-Reiss Tomer 
Published: August 2023

Abstract
The mechanisms underlying male infertility are poorly understood. Most mammalian spermatozoa have two centrioles: the typical barrel-shaped proximal centriole (PC) and the atypical fan-like distal centriole (DC) connected to the axoneme (Ax). These structures are essential for fertility. However, the relationship between centriole quality and subfertility (reduced fertility) is not well established. Here, we tested the hypothesis that assessing sperm centriole quality can identify cattle subfertility. By comparing sperm from 25 fertile and 6 subfertile bulls, all with normal semen analyses, we found that unexplained subfertility and lower sire conception rates (pregnancy rate from artificial insemination in cattle) corelate with abnormal centriolar biomarker distribution. Fluorescence-based Ratiometric Analysis of Sperm Centrioles (FRAC) found only four fertile bulls (4/25, 16%) had positive FRAC tests (having one or more mean FRAC ratios outside of the distribution range in a group’s high-quality sperm population), whereas all of the subfertile bulls (6/6, 100%) had positive FRAC tests (P=0.00008). The most sensitive biomarker was Acetylated Tubulin, which had a novel labeling pattern between the DC and Ax. These data suggest that FRAC and Acetylated Tubulin labeling can identify bull subfertility that remains undetected by current methods and may provide insight into a novel mechanism of subfertility.
Survey on Drosophila suzukii and Ceratitis capitata (Diptera: Drosophilidae, Tephritidae) and associated Eucoilinae species (Hymenoptera: Figitidae) in northwestern Argentina. First record of Dicerataspis grenadensis and Leptopilina boulardi as parasitoids of D. suzukii
Authors: Reche Vanina Anadina , Biancheri Maria Josefina Buonocore , Cao Luciana Marina , Suárez Lorena del Carmen , Ovruski Sergio Marcelo , Kirschbaum Daniel Santiago , Garcia Flávio Roberto Mello , Gallardo Fabiana Edith 
Published: August 2023

Abstract

The Southeast Asian-nativeDrosophila suzukii(Matsumura) (Diptera: Drosophilidae), also known as “spotted-wing Drosophila”, is one of the most globally invasive agricultural species. AlthoughD. suzukiiis a pest spread throughout all the Argentinian fruit-growing regions, few information has been published on its impact on local fruit production. Parasitoid species associated withD. suzukiiin Argentina belong to Pteromalidae (Chalcidoidea), Diapriidae (Diaprioidea), both attacking host pupae, and Figitidae (Cynipoidea), which attacks host larvae. Nine Eucoilinae (Figitidae) species, belonging toDicerataspis, Dieucoila, Euxestophaga, Ganaspis, HexacolaandLeptopilinagenera, have been associated withD. suzukiiin Argentina,.Ceratitis capitata(Wiedemann), commonly known as “medfly”, is native to Africa and has a worldwide distribution, covering many tropical, subtropical and temperate regions. In Argentina,C. capitatahas been associated with several native hymenopterous parasitoids belonging to Braconidae (Ichneumonioidea), Eulophidae (Chalcidoidea), Pteromalidae, Diapriidae and Figitidae families. Only two eucoline species,Ganaspis pelleranoi(Brèthes) andRhoptromeris haywardi(Blanchard) have been related to medfly in Argentina. We report new trophic associations between the parasitoidsDicerataspis grenadensisAshmead andLeptopilina boulardi(Barbotin, Carton and Kelner-Pillault) andD. suzukii, andbetween the parasitoidOdontosema albinerveKieffer andC. capitata, after surveys conducted in Tucumán, northwestern Argentina. An annotated checklist and a taxonomic key of Eucoilinae associated with both invasive pests, in Argentina, is also provided.
Adaptive immune changes associate with clinical progression of Alzheimer’s disease
Authors: Olst Lynn van , Kamermans Alwin , Halters Sem , Pol Susanne M.A. van der , Rodriguez Ernesto , Verberk Inge M.W. , Verberk Sanne G.S. , Wessels Danielle W.R. , Rodriguez-Mogeda Carla , Verhoeff Jan , Wouters Dorine , Bossche Jan Van den , Garcia-Vallejo Juan J. , Lemstra Afina W. , Witte Maarten E. , Flier Wiesje M. van der , Teunissen Charlotte C.E. , de Vries Helga E. 
Published: August 2023

AbstractBackground: Alzheimer’s disease (AD) is the most frequent cause of dementia. Recent evidence suggests the involvement of peripheral immune cells in the disease, but the underlying mechanisms remain unclear. Methods: We comprehensively mapped peripheral immune changes in AD patients with mild cognitive impairment (MCI) or dementia compared to controls, using cytometry by time-of-flight (CyTOF). Results: We found an adaptive immune signature in AD, and specifically highlight the accumulation of PD1+CD57+CD8+T effector memory cells re-expressing CD45RA in the MCI stage of AD. In addition, several innate and adaptive immune cell subsets correlated to CSF biomarkers of AD neuropathology and measures for cognitive decline. Intriguingly, subsets of memory T and B cells were negatively associated with CSF biomarkers for tau pathology, neurodegeneration and neuroinflammation in AD patients. Lastly, we established the influence of the APOE ε4 allele on peripheral immunity. Conclusions: Our findings illustrate significant peripheral immune alterations associated with both early and late clinical stages of AD, emphasizing the necessity for further investigation into how these changes influence underlying brain pathology.
Predicting Dimensional Antidepressant Response to Repetitive Transcranial Magnetic Stimulation using Pretreatment Resting-state Functional Connectivity
Authors: Wade Benjamin , Barbour Tracy , Ellard Kristen , Camprodon Joan 
Published: August 2023

Abstract
Repetitive transcranial magnetic stimulation (rTMS) is an effective treatment for depression and has been shown to modulate resting-state functional connectivity (RSFC) of depression-relevant neural circuits. To date, however, few studies have investigated whether individual treatment-related symptom changes are predictable from pretreatment RSFC. We use machine learning to predict dimensional changes in depressive symptoms using pretreatment patterns of RSFC. We hypothesized that changes in dimensional depressive symptoms would be predicted more accurately than scale total scores. Patients with depression (n=26) underwent pretreatment RSFC MRI. Depressive symptoms were assessed with the 17-item Hamilton Depression Rating Scale (HDRS-17). Random forest regression (RFR) models were trained and tested to predict treatment-related symptom changes captured by the HDRS-17, HDRS-6 and three previously identified HDRS subscales: core mood/anhedonia (CMA), somatic disturbances, and insomnia. Changes along the CMA, HDRS-17, and HDRS-6 were predicted significantly above chance, with 9%, 2%, and 2% of out-of-sample outcome variance explained, respectively (all p<0.01). CMA changes were predicted more accurately than the HDRS-17 (p<0.05). Higher baseline global connectivity (GC) of default mode network (DMN) subregions and the somatomotor network (SMN) predicted poorer symptom reduction, while higher GC of the right dorsal attention (DAN) frontoparietal control (FPCN), and visual networks (VN) predicted reduced CMA symptoms. HDRS-17 and HDRS-6 changes were predicted with similar GC patterns. These results suggest that RSFC spanning the DMN, SMN, DAN, FPCN, and VN subregions predict dimensional changes with greater accuracy than syndromal changes following rTMS. These findings highlight the need to assess more granular clinical dimensions in therapeutic studies, particularly device neuromodulation studies, and echo earlier studies supporting that dimensional outcomes improve model accuracy.