bluuebunny/crossref_metadata_2025_split huggingface dataset:
The subacromial bursa is a key regulator of the rotator cuff and a new therapeutic target for improving repair
Authors: Marshall Brittany P. , Ferrer Xavier E. , Kunes Jennifer A. , Innis Astia C. , Luzzi Andrew J. , Forrester Lynn Ann , Burt Kevin G. , Lee Andy J. , Song Lee , Hung Clark T. , Levine William N. , Kovacevic David , Thomopoulos Stavros 
Published: July 2023

AbstractRotator cuff injuries result in over 500,000 surgeries performed annually, an alarmingly high number of which fail. These procedures typically involve repair of the injured tendon and removal of the subacromial bursa. However, recent identification of a resident population of mesenchymal stem cells and inflammatory responsiveness of the bursa to tendinopathy indicate an unexplored biological role of the bursa in the context of rotator cuff disease. Therefore, we aimed to understand the clinical relevance of bursa-tendon crosstalk, characterize the biologic role of the bursa within the shoulder, and test the therapeutic potential for targeting the bursa. Proteomic profiling of patient bursa and tendon samples demonstrated that the bursa is activated by tendon injury. Using a rat to model rotator cuff injury and repair, tenotomy-activated bursa protected the intact tendon adjacent to the injured tendon and maintained the morphology of the underlying bone. The bursa also promoted an early inflammatory response in the injured tendon, initiating key players in wound healing.In vivoresults were supported by targeted organ culture studies of the bursa. To examine the potential to therapeutically target the bursa, dexamethasone was delivered to the bursa, prompting a shift in cellular signaling towards resolution of inflammation in the healing tendon. In conclusion, contrary to current clinical practice, the bursa should be retained to the greatest extent possible and provides a new therapeutically target for improving tendon healing outcomes.One Sentence SummaryThe subacromial bursa is activated by rotator cuff injury and regulates the paracrine environment of the shoulder to maintain the properties of the underlying tendon and bone.
Deep learning microstructure estimation of developing brains from diffusion MRI: a newborn and fetal study
Authors: Kebiri Hamza , Gholipour Ali , Vasung Lana , Krsnik Željka , Karimi Davood , Bach Cuadra Meritxell 
Published: July 2023

ABSTRACTDiffusion-weighted magnetic resonance imaging (dMRI) is widely used to assess the brain white matter. Fiber orientation distribution functions (FODs) are a common way of representing the orientation and density of white matter fibers. However, with standard FOD computation methods, accurate estimation of FODs requires a large number of measurements that usually cannot be acquired for newborns and fetuses. We propose to overcome this limitation by using a deep learning method to map as few as six diffusion-weighted measurements to the target FOD. To train the model, we use the FODs computed using multi-shell high angular resolution measurements as target. Extensive quantitative evaluations show that the new deep learning method, using significantly fewer measurements, achieves comparable or superior results to standard methods such as Constrained Spherical Deconvolution. We demonstrate the generalizability of the new deep learning method across scanners, acquisition protocols, and anatomy on two clinical datasets of newborns and fetuses. Additionally, we compute agreement metrics within the HARDI newborn dataset, and validate fetal FODs with post-mortem histological data. The results of this study show the advantage of deep learning in inferring the microstructure of the developing brain from in-vivo dMRI measurements that are often very limited due to subject motion and limited acquisition times, but also highlight the intrinsic limitations of dMRI in the analysis of the developing brain microstructure. These findings, therefore, advocate for the need for improved methods that are tailored to studying the early development of human brain.
Insight into perfluorooctanoic acid-induced impaired mouse embryo implantation via single cell RNA-seq
Authors: Sheng Xiaoqiang 
Published: July 2023

AbstractPFOA (perfluorooctanoic acid) is a difficult-to-degrade chemical that poses significant risks to human health and the environment. Studies have shown that PFOA affect female reproduction, but effect and mechanism of low doses PFOA expose on endometrial receptivity are unclear. In this study, we found that exposure to low doses of PFOA damaged endometrial receptivity in mice, resulting in decreased embryo implantation rates in mice. Furthermore, using single-cell sequencing technology, we systematically analyzed the specific mechanisms by which PFOA damages endometrial epithelial cell function and the ANGTL signaling pathway between endometrial stromal cells and epithelial cells, leading to embryo implantation failure. The elucidation of this mechanism provides new targets for the treatment of infertility about exposed to PFOA.
ROM1 is redundant to PRPH2 as a molecular building block of photoreceptor disc rims
Authors: Lewis Tylor R. , Makia Mustafa S. , Castillo Carson M. , Hao Ying , Al-Ubaidi Muayyad R. , Skiba Nikolai P. , Conley Shannon M. , Arshavsky Vadim Y. , Naash Muna I. 
Published: July 2023

AbstractVisual signal transduction takes place within a stack of flattened membranous “discs” enclosed within the light-sensitive photoreceptor outer segment. The highly curved rims of these discs, formed in the process of disc enclosure, are fortified by large hetero-oligomeric complexes of two homologous tetraspanin proteins, PRPH2 (a.k.a. peripherin-2 or rds) and ROM1. While mutations in PRPH2 affect the formation of disc rims, the role of ROM1 remains poorly understood. In this study, we found that the knockout of ROM1 causes a compensatory increase in the disc content of PRPH2. Despite this increase, discs of ROM1 knockout mice displayed a delay in disc enclosure associated with a large diameter and lack of incisures in mature discs. Strikingly, further increasing the level of PRPH2 rescued these morphological defects. We next showed that disc rims are still formed in a knockin mouse in which the tetraspanin body of PRPH2 was replaced with that of ROM1. Together, these results demonstrate that, despite its contribution to the formation of disc rims, ROM1 can be replaced by an excess of PRPH2 for timely enclosure of newly forming discs and establishing normal outer segment structure.
Homologous mutations in β, embryonic, and perinatal muscle myosins have divergent effects on molecular power generation
Authors: Liu Chao , Karabina Anastasia , Meller Artur , Bhattacharjee Ayan , Agostino Colby J , Bowman Greg R , Ruppel Kathleen M , Spudich James A , Leinwand Leslie A 
Published: July 2023

AbstractMutations at a highly conserved homologous residue in three closely related muscle myosins cause three distinct diseases involving muscle defects: R671C in β-cardiac myosin causes hypertrophic cardiomyopathy, R672C and R672H in embryonic skeletal myosin cause Freeman Sheldon syndrome, and R674Q in perinatal skeletal myosin causes trismus- pseudocamptodactyly syndrome. It is not known if their effects at the molecular level are similar to one another or correlate with disease phenotype and severity. To this end, we investigated the effects of the homologous mutations on key factors of molecular power production using recombinantly expressed human β, embryonic, and perinatal myosin subfragment-1. We found large effects in the developmental myosins, with the most dramatic in perinatal, but minimal effects in β myosin, and magnitude of changes correlated partially with clinical severity. The mutations in the developmental myosins dramatically decreased the step size and load-sensitive actin-detachment rate of single molecules measured by optical tweezers, in addition to decreasing ATPase cycle rate. In contrast, the only measured effect of R671C in β myosin was a larger step size. Our measurements of step size and bound times predicted velocities consistent with those measured in an in vitro motility assay. Finally, molecular dynamics simulations predicted that the arginine to cysteine mutation in embryonic, but not β, myosin may reduce pre-powerstroke lever arm priming and ADP pocket opening, providing a possible structural mechanism consistent with the experimental observations. This paper presents the first direct comparisons of homologous mutations in several different myosin isoforms, whose divergent functional effects are yet another testament to myosin’s highly allosteric nature.
Karyological diversity among six medicinally important species of Acanthaceae in Bangladesh
Authors: Anjum Nowshin , Dash Chandan Kumar , Sultana Syeda Sharmeen 
Published: July 2023

ABSTRACTSix medicinally important species of Acanthaceae family namely,Asystasia gangetica(L.) T. Anderson,Barleria cristataL.,Braleria prionitisL.,Ruellia simplexC. Wright,Thunbergia erecta(Benth.) T. Anderson andThunbergia mysorensis(Wright) T. Anderson were cytogenetically investigated to evaluate the karyotype diversity and chromosomal relationship. The somatic chromosome numbers and the centromeric formulas of these species were 2n = 2x = 24 (22m + 2sm) forA. gangetica, 2n = 4x = 40 (38m + 2sm) forB. cristata, 2n = 4x = 40 (36m + 4sm) forB. prionitis, 2n = 2x = 36 (34m + 2sm) forR. simplex, 2n = 4x+6 = 62 (56m + 6sm) forT. erectaand 2n = 2x = 34 (34m) forT. mysorensis. Considering the karyotype assessment parameters,T. erecta, R. simplex, B. cristataandB. prionitiswere shown to be cytogenetially more advanced thanA. gangeticaandT. mysorensis. Three categories of karyotypes were observed such as 1B type inB. cristata, B. prionitis, R. simplexandT. mysorensis, whereas the most primitive one 1A inA. gangeticaand the most advanced karyotype 2B inT. erecta. Three new reports of somatic chromosome count yielded in case ofA. gangetica, R. simplexandT. mysorensiswhich might have been resulted from aneuploidy or allopolyploidy. The cytological data presented for six species of the Acanthaceae family demonstrated significant variation and distinctiveness from one another, assisting in the evaluation of the karyological diversity that aided in the understanding of evolutionary changes.
Auxin signaling in the cambium promotes tissue attachment and vascular development duringArabidopsis thalianagraft formation
Authors: Serivichyaswat Phanu T. , Kareem Abdul , Feng Ming , Melnyk Charles W. 
Published: July 2023

AbstractThe remarkable ability of plants to regenerate wounds is exemplified during the process of plant grafting when two plants are cut and joined together to grow as one. During graft healing, damaged tissues attach, cells proliferate and the vasculatures connect to form a graft union. The plant hormone auxin plays a central role and mutants perturbed in auxin response fail to successfully graft. Here, we investigated the role of individual cell types and their response to auxin duringArabidopsis thalianagraft formation. By employing an inducible misexpression system, we blocked auxin response in individual cell types using thebodenlosmutation. We found that auxin signaling in procambial tissues was critical for both successful tissue attachment and also for vascular differentiation. In addition, we found that auxin signaling was required for cell divisions of the procambial cells during graft formation. Loss of function mutants in cambial pathways also perturbed attachment and phloem reconnection. We propose that cambium and procambium are key tissues that allow both tissue attachment and vascular differentiation during successful grafting. Our study thus refines our knowledge of graft development and furthers our understanding of regeneration biology and the function of cambium.
Rapid assessment of changes in phage bioactivity using dynamic light scattering
Authors: Dharmaraj Tejas , Kratochvil Michael J. , Pourtois Julie D. , Chen Qingquan , Hajfathalian Maryam , Hargil Aviv , Lin Yung-Hao , Evans Zoe , Oromí-Bosch Agnès , Berry Joel D. , McBride Robert , Haddock Naomi L. , Holman Derek R. , van Belleghem Jonas D. , Chang Tony H. , Barr Jeremy J. , Lavigne Rob , Heilshorn Sarah C. , Blankenberg Francis G. , Bollyky Paul L. 
Published: July 2023

AbstractExtensive efforts are underway to develop bacteriophages as therapies against antibiotic-resistant bacteria. However, these efforts are confounded by the instability of phage preparations and a lack of suitable tools to assess active phage concentrations over time. Here, we use Dynamic Light Scattering (DLS) to measure changes in phage physical state in response to environmental factors and time, finding that phages tend to decay and form aggregates and that the degree of aggregation can be used to predict phage bioactivity. We then use DLS to optimize phage storage conditions for phages from human clinical trials, predict bioactivity in 50-year-old archival stocks, and evaluate phage samples for use in a phage therapy/wound infection model. We also provide a web-application (Phage-ELF) to facilitate DLS studies of phages. We conclude that DLS provides a rapid, convenient, and non-destructive tool for quality control of phage preparations in academic and commercial settings.Significance StatementPhages are promising for use in treating antibiotic-resistant infections, but their decay over time in refrigerated storage and higher temperatures has been a difficult barrier to overcome. This is in part because there are no suitable methods to monitor phage activity over time, especially in clinical settings. Here, we show that Dynamic Light Scattering (DLS) can be used to measure the physical state of phage preparations, which provides accurate and precise information on their lytic function – the key parameter underlying clinical efficacy. This study reveals a “structure-function” relationship for lytic phages and establishes DLS as a method to optimize the storage, handling, and clinical use of phages.
Management control practices and pragmatism
Authors: Curtis Emer , Sweeney Breda 
Published: July 2023

PurposePragmatism is very relevant to workplace management and performance measurement, yet in the accounting literature, it is a term used loosely and in a colloquial manner. By drawing on a framework based on classical pragmatism, this study aims to examine how a pragmatic perspective is discernible in the form and use of management control (MC) practices.Design/methodology/approachThis study collects data using a case study of a firm in the green energy construction sector.FindingsBuilding on the analytical framework, this study provides evidence that a pragmatic perspective is discernible in both form and use of MC practices, through a clear focus on targets rather than variance analysis, the presence of mutable local MC practices characterised by interaction and problem-solving and the absence of other common MC practices with no clear links to ends-in-view. This study also provides evidence of the potential limitations of a pragmatic perspective including myopia and an exacerbation of the inherent bias in organisations towards exploitation.Originality/valueThis research brings analytical clarity to the study of pragmatism in the accounting literature and insights into how a pragmatic perspective is discernible in the form and use of MC practices. Further, the study shows the potential limitations of a pragmatic perspective for management.
Review of drivers of teamwork for construction health and safety
Authors: Shaikh Aziz Yousif , Osei- kyei Robert , Hardie Mary , Stevens Matt 
Published: July 2023

PurposeThis paper systematically reviewed research work on drivers of teamwork, which will reinforce construction work teams to enhance workers’ safety performance. This study adds to the existing but limited understanding of teamwork drivers on construction workers’ safety performance. This paper presents scholars and industry-based professionals with critical initiatives that have to be implemented in organisations to get positive results in safety while working in teams with an emphasis on systems drivers of teamwork on safety performance at the organisational level, which will help in providing information on the functioning of the teams and contribute towards improved safety performance of team workers.Design/methodology/approachThis study aims to systematically examine the existing body of knowledge on drivers of teamwork by analysing 53 publications from the years 1997–2021. The Scopus search engine was used to conduct a systematic review and germane publications were collated.FindingsAccording to the findings of the review, since 1997, there has been a burgeoning concern in the research of drivers of teamwork and its impact on workers’ safety performance. After performing a systematic review, 37 drivers of teamwork were identified. The top five drivers are effective communications, team workers’ relations, leadership, shared knowledge and information, and team training. Moreover, it was noted that the United States and Australia have been the international regions of focus for most of the research in the area of drivers of teamwork from the years 1997–2021. The 37 drivers of teamwork are distributed into six major socio-technical components: people drivers; culture drivers; metrics drivers; organisational and management practices and procedures drivers; infrastructure drivers and technology drivers.Practical implicationsThe results reported present research scholars and professional practitioners with an overview of the drivers of teamwork that could be implemented in the construction industry to streamline potential implementations and improve safety performance of construction workers.Originality/valueA list of teamwork drivers has been developed to ratify potential empirical research in the area of construction safety. The results would contribute to the existing but restricted understanding of drivers of teamwork in the construction industry.
New insights into the impact of global security on economic growth: The case of Tunisia
Authors: Jmaii Amal , Zaafouri Noomene 
Published: July 2023

PurposeThis paper provides a new empirical evidence of the impact of Global security on economic growth in Tunisia. Like Buzan (1991), the framework used four types of security: army security, economic security, social security, and political security.Design/methodology/approachThe authors examine the multidimensionality of global security using ARDL-based cointegration bound tests. The model allows also for examining the long-run/short-run impacts of global security indicators on economic growth.FindingsThe proposed methodology revealed interesting results that may raise some potentially prominent policy implications. First, the findings show that the four security indicators have a significant impact on economic growth. In particular, from a social/political security point of view, the fundamental objective is to satisfy the needs of the poorest population, to limit the effect of social demands and protests on economic and political stability, to review the subsidy principle and aid policy to the poorest families, for greater efficiency and social equity.Originality/valueThe study highlights that global security is no longer a question of just army and defense, but it is also a question of equity, economic, social, and political development. These results call for the policymaker to adopt a strategy that reduces regional disparities and social inequalities and this through fairly distributing financial wealth for the benefit of marginalized populations.Peer reviewThe peer review history for this article is available at: https://publons.com/publon/10.1108/IJSE-02-2023-0082
The impact of interaction on continuous use in online learning platforms: a metaverse perspective
Authors: Xu Wei , Zhang Nan , Wang Mingming 
Published: July 2023

PurposeAs online learning is the embryonic form of education in the metaverse, it is extremely important to explore the behavioral preferences of users. The aim is to explore the impact of interactive features on continuous use in online learning and to further explore what kind of interaction mode should be constructed for different types of students to obtain the best educational experience.Design/methodology/approachThe study developed an empirical model and used a real-world dataset to test hypotheses. Specifically, the interaction in online learning is analyzed from different dimensions, including the interaction intensity of multiple subjects, the immersion of interactive technology, the timeliness of interactive feedback, and the fun in interaction.FindingsThe authors found that the intensity of interaction, immersion, timeliness of feedback and fun in the interaction all had significant positive effects on continuous use. Among them, the most important is the interaction between teachers and students. With the growth of user grades, the role of parents in the interaction is getting smaller and smaller, and the fun in the interaction is gradually becoming unnecessary. For high school students, gamified interactions can even have a negative impact. In addition, from the perspective of gender, males prefer immersive interaction, while females pay more attention to themselves and have negative feedback on fees.Originality/valueThe authors deepened the interaction and summarized the impact of different interactive features on continuous use in online learning platforms. The authors focused on the impact of the immersive experience brought by the application of interactive technology, which can confirm the user behavior preferences of online learning in the context of the metaverse. The research also provides a reference for online learning institutions to set up course interaction modes and targeted marketing programs.
Rasa indung bini: an exploratory research into Bruneian sense of motherhood
Authors: Sultana Rumana 
Published: July 2023

Purpose This is exploratory research on the Bruneian sense of motherhood. The pre-socially established religious definition of motherhood is unformulated where; the analysis is located in the inner context of experience. The Western feminist idea defined motherhood as such reality as the “otherness” whereby its common sense justifies sexuality and feminism. The religious significance gives meaning to its spiritual connection, embodying a foundational knowledge of Bruneian proper personhood – being a good, spiritual and respected person. This relates to the importance of the body as an entity of both culture and symbol that has drawn attention to its sociological significance.Design/methodology/approach The argument here is how religious experience influences the meaning of motherhood in Brunei Darussalam, which underwent a transformation scheme to empower women. Participants were interviewed, and the transcribed interviews were analysed using the reflexive methodology to find out the natural phenomena of motherhood.Findings The findings indicate that the Bruneian sense of motherhood is regulated through natural norms of femininity, an unformulated knowledge of spirituality, religion and the influence of cultural and religious rituals, such as prayers, consuming pure food, concoction and reciting holy verses from the Quran.Originality/value This study reveals to codify and extend the sense of religious obedience through generations. Religion rationalises the spiritual experience and provides the basis of obedience towards a proper development of personhood Bruneian. The idea of motherhood itself recreates a role to empower women, which will initiate opportunities for further studies in Brunei context.