bluuebunny/crossref_metadata_2025_split huggingface dataset:
Integrating genome wide association studies with selective sweep reveals genetic loci associated with tolerance to low phosphate availability in Brassica napus
Authors: Liu Haijiang , Yuan Pan , Cui Rui , Hammond John P , White Philip J. , Zhang Yuting , Zou Maoyan , Ding Guangda , Wang Sheliang , Cai Hongmei , Xu Fangsen , SHI LEI 
Published: February 2023

Abstract
Oilseed rape (Brassica napus L.; B. napus) is an important oil crop around the world. However, the genetic mechanism of B. napus adaptations to low phosphate (P) stress are largely unknown. In this study, a genome-wide association study (GWAS) identified 68 SNPs significantly associated with seed yield (SY) under low P (LP) availability in two trials. Among these SNPs, two, chrC07__39807169 and chrC09__14194798, were co-detected in two trials, and BnaC07.ARF9 and BnaC09.PHT1;2 were identified as candidate genes of them, respectively, by combine GWAS with quantitative reverse-transcription PCR (qRT-PCR). There were significant differences in the gene expression level of BnaC07.ARF9 and BnaC09.PHT1;2 between P -efficient and -inefficiency varieties at LP. SY_LP had a significant positive correlation with the gene expression level of both BnaC07.ARF9 and BnaC09.PHT1;2. BnaC07.ARF9 and BnaA01.PHR1 could directly bind the promoters of BnaA01.PHR1 and BnaC09.PHT1;2, respectively. Selective sweep analysis was conducted between ancient and derived B. napus, and detected 1280 putative selective signals. Within the selected region, a large number of genes related to P uptake, transport and utilization were detected, such as purple acid phosphatase (PAP) family genes and phosphate transporter (PHT) family genes. These findings provide novel insights into the molecular targets for breeding P efficiency varieties in B. napus.
Adsorption and mitigation impact of the monosodium glutamate (C₅H₈NO₄Na) bio-molecules on the steel rebar corrosion in the chloride-contaminated simulated concrete pore solution
Authors: Mohammadkhah Sahel , Dehghani Ali , Ramezanzadeh Bahram 
Published: February 2023

Abstract
Corrosion has caused significant annual costs for building construction and civil architectural designs. In this study, Monosodium glutamate (GLU) was proposed as a potential candidate for long-lasting corrosion inhibition to slow down the rate of corrosion in the concrete pore environment. In this regard, the electrochemical and morphological properties of the various GLU concentrated systems between 1 to 5 wt.% in the simulated concrete pore solution media were investigated. According to the EIS results, adding 4 wt.% of GLU could reduce the mild steel corrosion process by 86% through a mixed inhibition mechanism. Also, the polarization records represented that the samples' corrosion current density was diminished to 0.169 µA.cm-2 after the addition of 4 wt.% GLU into the harsh environment. Using the FE-SEM method, the growth of the GLU layer over the metal substrate was demonstrated. The results of spectroscopic methods, i.e., Raman and GIXRD, demonstrated that GLU molecules were successfully adsorbed over the surface of the metal. Contact angle test outcomes showed that by increasing the GLU concentration to its optimum level (4 wt.%), the surface hydrophobicity was dramatically raised to 62 degrees.
N-Oleoyl Glycine and N-Oleoyl Alanine Attenuate Alcohol Self-Administration and Preference in Mice
Authors: Yaka Rami , Shahen-Zoabi Samah , Smoum Reem , Bingor Alexey , Grad Etty , Nemirovski Alina , Shekh-Ahmad Tawfeeq , Mechoulam Raphael 
Published: February 2023

Abstract
The endocannabinoid system (ECS) plays a key modulatory role during synaptic plasticity and homeostatic processes in the brain, and has an important role in the neurobiological processes underlying drug addiction. We have previously shown that an elevated ECS response to psychostimulant (cocaine) is involved in regulating the development and expression of cocaine-conditioned reward and cocaine sensitization. Here, we determine the involvement of ECS in alcohol addiction. We first measured the levels of endocannabinoids (eCBs) and eCB-like molecules in different regions of the brain reward system following chronic alcohol self-administration using LCMS. We found that following chronic intermittent alcohol consumption, N-oleoyl glycine (OlGly) levels were significantly elevated in the prefrontal cortex (PFC), and N-oleoyl alanine (OlAla) was significantly elevated in the PFC, nucleus accumbens (NAc) and ventral tegmental area (VTA) in a region-specific manner. We next tested whether exogenous administration of OlGly or OlAla would attenuate alcohol consumption and preference. We found that systemic administration of OlGly or OlAla (60mg/kg, intraperitoneal) during intermittent alcohol consumption significantly reduced alcohol intake and preference. These findings suggest that the ECS negatively regulates alcohol consumption and boosting selective endocannabinoids exogenously has beneficial effects against alcohol consumption and potentially in preventing relapse.
Discrimination training affects stimulus generalization in mice during Pavlovian eyeblink conditioning
Authors: Fiocchi Francesca Romana , Dorp Nikki van , Dijkhuizen Stephanie , Berg Maurits van der , Wong Aaron , Jung Caroline , de Zeeuw Chris I. , Boele Henk-Jan 
Published: February 2023

Abstract
The delicate balance between generalization and discrimination of responses is crucial for survival in our ever-changing environment. In particular, it is important to understand how stimulus discrimination affects the level of stimulus generalization. For example, when we use non-differential training for Pavlovian eyeblink conditioning to investigate generalization of cerebellar-related eyelid motor responses, we find generalization effects on both amplitude and timing of the conditioned responses. However, it is unknown what the generalization effects are following differential training. We trained mice to close their eyelids to a 10kHz tone (CS+) while alternatingly exposing them to a tone frequency of either 4kHz, 9kHz or 9.5kHz (CS-). We tested the generalization effects with tones ranging from 2kHz to 20kHz. Our results show that the level of generalization tended to positively correlate with the difference between the CS+ and the CS- training stimuli. These effects of generalization were only found for the probability and amplitude of the conditioned responses, but we did not find any significant effect on the onset of the conditioned eyelid responses. These data highlight the specificity of the generalization effects following differential versus non-differential training.
Assessing Variants of Uncertain Significance Implicated in Hearing Loss Using a Comprehensive Deafness Proteome
Authors: Tollefson Mallory R. , Gogal Rose A. , Weaver A. Monique , Schaefer Amanda M. , Marini Robert J. , Azaiez Hela , Kolbe Diana L. , Wang Donghong , Weaver Amy E. , Casavant Thomas L. , Braun Terry A. , Smith Richard J. H. , Schnieders Michael 
Published: February 2023

Abstract
Hearing loss is the leading sensory deficit, affecting ~ 5% of the population. It exhibits remarkable heterogeneity across 223 genes with 6,328 pathogenic missense variants, making deafness-specific expertise a prerequisite for ascribing phenotypic consequences to genetic variants. Deafness-implicated variants are curated in the Deafness Variation Database (DVD) after classification by a genetic hearing loss expert panel and thorough informatics pipeline. However, seventy percent of the 128,167 missense variants in the DVD are “variants of uncertain significance” (VUS) due to insufficient evidence for classification. Here, we use the deep learning protein prediction algorithm, AlphaFold2, to curate structures for all DVD genes. We refine these structures with global optimization and the AMOEBA force field and use DDGun3D to predict folding free energy differences (∆∆GFold) for all DVD missense variants. We find that 5,772 VUSs have a large, destabilizing ∆∆GFold that is consistent with pathogenic variants. When also filtered for CADD scores (> 25.7), we determine 3,456 VUSs are likely pathogenic at a probability of 99.0%. These VUSs affect 119 patients (~ 3% of cases) sequenced by the OtoSCOPE targeted panel. Approximately half of these patients previously received an inconclusive report, and reclassification of these VUSs as pathogenic provides a new genetic diagnosis for six patients.
A New Cuproptosis-Related LncRNAs Prognostic Signature of Cervical Cancer
Authors: Liao Fuchun , Yin Weijiao , Yao Desheng 
Published: February 2023

Abstract
Cervical cancer (CC) is the most common gynecological tumor in the world. Cuproptosis is programmed cell death caused by increased intracellular concentrations of Cu2+. The aim of this study was to establish a new cuproptosis-related lncRNAs prognostic signature of cervical cancer to predict prognosis and guide treatment. Our study was the first to identify a cuproptosis-related lncRNAs in cervical cancer and established a cuproptosis-related lncRNAs prognostic signature. We also used qPCR to verify the relationship between these lncRNAs and cuproptosis in cervical cancer. This signature can be used to predict prognosis and response to immunotherapy in patients with cervical cancer. In addition, we found that there were differences in immune cell infiltration, immune function, and m6A between the high-risk and low-risk groups, and Patients in different risk groups have different sensitivities to small-molecule drug therapy. In addition, we build a nomogram model based on the risk model and clinical features, which is shown to have excellent predictive power. In summary, we have developed a novel prognostic cuproptosis-related lncRNAs signature that could provide new ideas for the treatment of cervical cancer patients.
Machine learning-based model for predicting the outcome of abdominal paracentesis drainage
Authors: Liu Ruohong , Zhang Yufan , He Liang , Huang Zhu , Wen Yi , Li Yunming , Tang Lijun 
Published: February 2023

Abstract
Background
Abdominal Paracentesis drainage (APD) is a useful treatment for acute pancreatitis (AP) patient with pancreatitis associated ascitic fluid, however, researches seldom mentioned whether every patient benefit from this treatment. Here, we described a machine learning model to predict the outcomes of APD on certain AP patients.
Methods
The EHR data of 464 AP patients admitted between 2014 to 2020 were used in our study in a de-identified way. A machine learning model using random forest algorithm was established and validated under the stratified 10 fold cross validation strategy. The patients were labelled as “apd_cure” and “apd_serious” group according to their outcome, and the accuracy, sensitivity, specificity, positive prediction value, negative prediction value and ROC curve as well as its area under curve were used to value the efficacy of the model. A logistic regression model was established in the same strategy to compared their predictability.
Results
The random forest model has an excellent overall properties in predicting the outcomes of APD treatment for the AUC was 0.703 ± 0.118 [95%CI 0.64–0.77]. The accuracy, specificity and NPV (Negative Predictive Value) of the model was 0.786 ± 0.038, 0.940 ± 0.037 and 0.817 ± 0.037, respectively, indicates the model was more able to correctly classify patients who improved after APD treatment. The sensitivity and PPV(Positive Predictive Value) of the model was 0.208 ± 0.144 and 0.486 ± 0.232, which means that the model has insufficient ability to identify patients who may be more likely to have a worsening condition after APD treatment. Finally, the random forest model was statistically better than logistic regression model in accuracy and specificity.
Conclusion
The random forest model described in this study is a validated model in predicting the outcome of APD treatment on acute pancreatitis patients. It has higher overall performance than the logistic regression model. We hope it may help doctors choose treatment options appropriately and may enhance treatment efficacy in this group of patients.
Noncanonical Il-1β maturation in microglia impairs cognition in chronic kidney disease via neuronal IL-1R signaling
Authors: zimmermann silke , Mathew Akash , Bondareva Olga , Elwakiel Ahmed , Waldmann Klarina , Jiang Shihai , Rana Rajiv , Singh Kunal , Kohli Shrey , Shahzad Khurrum , Biemann Ronald , Roskoden Thomas , Storsberg Silke , Mawrin Christian , Krügel Ute , Bechmann Ingo , Goldschmidt Jürgen , Sheikh Bilal , Isermann Berend 
Published: February 2023

Abstract

While cognitive impairment is common in peripheral diseases such as chronic kidney disease (CKD), mechanistic insights and effective therapies are lacking1,2. Organ substitution reverses cognitive impairment, indicating that cognitive impairment driven by peripheral diseases is therapeutically amendable3,4. Yet, we lack pharmacological approaches. Here, we show that despite inflammasome activation in the brain and protection in full body NLRP3-inflammasome deficient mice, microglial caspase-1 deficiency does not improve inflammation and cognition in CKD mice. Microglial potassium (K+) dyshomeostasis induces noncanonical, cathepsin C–caspase-8 mediated IL-1β maturation and neuronal dysfunction via IL-1R signaling in CKD. Neuronal IL-1R-signaling induces neuronal NLRP3 expression, indicating an unrecognized function of NLRP3 in neurons. Restoring K+ homeostasis in microglia using a KCa3.1-specific inhibitor (TRAM34) or genetically inhibiting neuronal IL-1R1 signaling improves CKD-triggered cognitive impairment. These insights identify a new intercellular microglia IL-1β –neuron IL-1R1 crosstalk and identify potential therapeutic targets to combat neuronal dysfunction in peripheral diseases.
Annotated sequence First report of Soybean chlorotic blotch virus infecting Okra (Abelmoschus esculentus) in Cameroon
Authors: Lauryn Bahoya JA. , Piau Jean CM. , Monono Ekwa Y. , Nkeabeng Leke Walter 
Published: February 2023

Abstract

Leaves from okra (Abelmoschus esculentus) plants exhibiting unusual viral disease symptoms were tested for the presence of begomovirus infection. The putative full-length Soybean chlorotic blotch virus (SbCBV) components A and B molecules, respectively were amplified, sequenced, and analyzed. This is the first study identifying SbCBV, a bipartite begomovirus infecting okra in Cameroon and the centra Central African region.
Caring for older patients with reduced decision-making capacity. A deductive exploratory study of ambulance clinicians’ ethical competence
Authors: Holmberg Bodil , Bennesved Anna , Bremer Anders 
Published: February 2023

Abstract
Background As more people are living longer, they become frail and are affected by multi-morbidity, resulting in increased demands from the ambulance service. Being vulnerable, older patients may have reduced decision-making capacity, despite still wanting to be involved in decision-making about their care. Their needs may be complex and difficult to assess, and do not always correspond with ambulance assessment protocols. When needing an ambulance, older patients encounter ambulance clinicians who are under high workloads and primarily consider themselves as emergency medical care providers. This situates them in the struggle between differing expectations, and ethical conflicts may arise. To resolve these, providing ethical care, focussing on interpersonal relationships and using ethical competence is needed. However, it is not known whether ambulance clinicians possess the ethical competence required to provide ethical care. Thus, the aim of this study was to deductively explore their ethical competence when caring for older patients with reduced decision-making ability.
Methods A qualitative deductive and exploratory design was used to analyse dyadic interviews with ambulance clinicians. A literature review, defining ethical competence as comprising ethical sensitivity, ethical knowledge, ethical reflection, ethical decision-making, ethical action and ethical behaviour, was used as a structured categorization matrix for the analysis.
Results Ambulance clinicians possess ethical competence in terms of their ethical knowledge, highlighting the need for establishing an interpersonal relationship with the older patient. To establish this, they use ethical sensitivity to interpret the patients’ needs. Doing this, they are aware of their ethical behaviour, signifying how they must act respectfully and provide the necessary time for listening and interacting.
Conclusions Ambulance clinicians fail to see their gut feeling as a professional ethical competence, which might hinder them from reacting to unethical ways of working. Further, they lack the acquired ethical knowledge regarding the benefits and disadvantages of paternalism, which reduces their ability to perform ethical decision-making. Moreover, their ethical knowledge is hampered by an ageist approach to older patients, which also has consequences for their ethical action. Finally, ambulance clinicians show deficiencies regarding their ethical reflections, as they reflect merely on their own actions, rather than on their values.
Clinical and genetic characterization of Neuronal ceroid lipofuscinoses (NCLs) in 29 Iranian patients: Identification of 11 novel mutations
Authors: Panjeshahi Samareh , Karimzadeh Parvaneh , Movafagh Abolfazl , Ahmadabadi Farzad , Rahimian Elham , Alijanpour Sahar , Miryounesi Mohammad 
Published: February 2023

Abstract
Neuronal ceroid lipofuscinoses (NCLs) are neurodegenerative lysosomal storage diseases witch considered among the most frequent cause of dementia in childhood worldwide This study aimed to identify the gene variants, molecular etiologies, and clinical features in 23 unrelated Iranian families with NCL. In total, 29 patients with Neuronal ceroid lipofuscinoses (NCLs), diagnosed based on clinical manifestations, MRI neuroimaging, and electroencephalography (EEG), were recruited for this study. through whole exome sequencing(WES), functional prediction, Sanger sequencing, and segregation analysis, we found that 12 patients (41.3%) with mutations in the CLN6 gene, 7 patients (24%) with the TPP1(CLN2) gene variants, and 4 patients (13.7%) with mutations in the MFSD8(CLN7) gene. Also, mutations in each of the CLN3 and CLN5 genes were detected in 2 cases and mutations of each PPT1(CLN1) and CLN8 gene were observed in only 1 separate patient. We identified 18 different mutations, 11 (61%) of which are novel, never have been reported before, and the others have been previously described. The gene variants identified in this study expand the number of published clinical cases and the variant frequency spectrum of the Neuronal ceroid lipofuscinoses (NCLs) genes; moreover, the identification of these variants supplies foundational clues for future NCL diagnosis and therapy.
Application of derivative UV spectrophotometry for the determination of marbofloxacin in pharmaceuticals
Authors: Elgendy Khaled Mansour , Zaky Monier Saad , turky alaa eldin , fadel alsayed 
Published: February 2023

Abstract
Background
A simple, rapid, accurate, and sensitive spectrometric method for the determination of Marbofloxacin has been developed. The present manuscript describes a first, second, third, and fourth derivative spectrophotometric method for the simultaneous estimation of and Marbofloxacin.
Results
First order derivative absorption at wavelengths of 300 nm and 278 nm, second order derivative absorption at wavelengths of 310 nm, 291 nm, and 270 nm, third order derivative absorption at wavelengths of 262 nm, 282 nm, 297 nm, and 311 nm, and fourth order derivative absorption at wavelengths of 258 nm, 267 nm, 292 nm, 304 (zero cross point for Marbofloxacin). With a correlation value (R2) of 0.994, marbofloxacin linearity was achieved in the concentration range of 2–20 µg/ml. The average recovery rate was found to be between 98.25 and 101.52%.
Conclusions
The suitability of these methods for the quantitative determination of Marbofloxacin was proved by validation. The proposed method was validated in accordance with the guidelines and was successfully applied to the simultaneous estimation of Marbofloxacin in its synthetic forms.
Low Profile Hybrid Frequency and Polarization Reconfigurable Bunny Patch Antenna for S-band Applications
Authors: Agarwal Sweta , Meshram Manoj Kumar 
Published: February 2023

Abstract

In this Article, a unique single probe feed Bunny patch antenna with hybrid frequency and polarisation reconfigurability for S –Band application is presented. A bunny patch antenna is a modified circular patch antenna. The bunny-shaped patch antenna is made by joining the conducting strips to the circular patch at 600. Two conducting strips, two notches on the patch periphery, and two RF-PIN diodes are used to reconfigure three polarisation states in this antenna: left-hand circular polarisation (LHCP), right-hand circular polarisation (RHCP), and linear polarisation (LP) with frequency reconfigurability. The dielectric material FR-4 is used, which has a thickness of 6.4mm and a permittivity of 4.4. This antenna features a 400 MHz, -10dB impedance bandwidth from 2.46–2.86 GHz and a 3.38% axial ratio (AR) bandwidth from 2.54–2.64 GHz. This antenna, when designed optimally, can emit a gain of about 5dBic in its circular polarization states. The proposed bunny patch antenna is fabricated and measured. Results have good agreement in all states of operation. The UMT (Universal MSS Terminal) receiver system can benefit from this antenna.
Paternal Age and risk of Congenital Anomalies and birth outcomes: A Population-based Cohort Study
Authors: Bu Xinghe , Ye Wenjing , Zhou Jianguo 
Published: February 2023

Abstract
Objective: To explore the impact of paternal age on the risk of congenital anomalies and birth outcomes in U.S. infants born between 2016 and 2021.
Methods: This retrospective cohort study was based on live births in the National Vital Statistics System database between 2016 and 2021. All newborns were divided into 4 groups based on paternal age (<25, 25-34, 35-44, and >44 years old groups) and using 25-34 years groups as reference. The primary outcomes were congenital anomalies including structural anomalies, and chromosome anomalies, while the secondary outcomes included preterm birth, low birth weight, severe neonatal perinatal asphyxia, admission to neonatal intensive care units, etc. The association between paternal age and outcomes was detected by multivariable logistic analysis.
Results: After the exclusion process, a total of 17,764,695 live births were included in the final analyses. After adjustment for confounding factors, compared with the paternal age of 25-34 years group, advanced paternal age of more than 44 years was associated with increased odds of congenital anomalies (aOR=1.17, 95%CI 1.12-1.21), mainly for the chromosomal anomalies (aOR=1.59, 95%CI 1.40-1.78) but not the structure anomalies (aOR=1.03, 95%CI 0.97-1.09). Advanced paternal age also increased the risk of preterm delivery, low birth weight, and admission to NICU in their infants.
Conclusion: Advanced paternal age increases the risk of congenital anomalies in their offspring, especially chromosomal anomalies, which implies more intensive prenatal or pre-conceptional investigation, including genetic tests, should be taken in the high-risk paternal populations.
Analytical solution for rock discontinuity prediction without stereonet
Authors: To Peter , Sivakugan Nagaratnam 
Published: February 2023

Abstract
Stereographic projection has been used in Rock Engineering for decades to represent rock mass discontinuities in graphical form and to carry out further stability analysis. Currently, there are computer programs available for this purpose. The objective of this technical note is to present some closed form expressions related to stereographic projections such as the prediction of a possible joint set. These solutions can be implemented in spreadsheets or in computer programs and would benefit stability analysis of rock masses with discontinuities. This helps to reduce the work load with hand solutions and enhance the analysis in modern computational tools with stereonet, where the rotation and snap features are not available yet.