bluuebunny/crossref_metadata_2025_split huggingface dataset:
Enhanced detection of viruses for improved water safety
Authors: Hayes Emalie , Gouthro Madison , Fuller Megan , Redden David , Gagnon Graham 
Published: June 2023

Abstract
Human viruses pose a significant health risk in freshwater environments, but current monitoring methods are inadequate for detecting viral presence efficiently. We evaluated a novel passive in-situ concentration method using granular activated carbon (GAC). This study detected and quantified eight enteric and non-enteric pathogenic viruses in a freshwater recreational lake in paired grab and GAC passive samples. Results found that GAC passive sampling had a higher detection rate for all viruses compared to grab samples, with adenovirus found to be the most prevalent virus, followed by respiratory syncytial virus, norovirus, enterovirus, Influenza A, SARS-CoV-2, and rotavirus. GAC in-situ concentration allowed for the capture and recovery of viral gene copy targets that ranged from one to three orders of magnitude higher than conventional ex-situ concentration methods used in viral monitoring. This simple and affordable sampling method may have far-reaching implications for reducing barriers associated with viral monitoring across various environmental contexts.
Selective binding and induction of death in leukemia cells by Biodegradable magnetized iron nanodiscs
Authors: Kharat Kiran , Pottathil Raveendran 
Published: June 2023

Abstract
Leukemia is a blood malignancy with a rate of new cases of 14.1 per 100,000 adults per year. Leukemic cells seek a way to escape from the cytotoxic effects of chemotherapy drugs, leading to treatment failure. In this paper, we demonstrated the separation of circulating leukaemia cells using an antibody-conjugated iron oxide nanodisc (MND-Ab) under a low magnetic field gradient. Biodegradable magnetic nanodiscs were prepared by using iron oxide, BSA and the antibody IPO-M6, which is an anti-HL-60 antibody. Atomic force microscope studies show that the nano-discs were sprayed uniformly with an average size of 22+/-2.1 nm. The efficiency of cell capture was monitored with Prussian blue staining and fluorescence microscopy. A bar magnet was placed near the plate at 2 mm distance from the centre of the plate. The labelled cells were gathered near the magnet after 2 minutes. When the MND-Ab-HL60 cells were exposed to magnetic oscillations (5 min) 10 htz, 90 Guass), 92–94% of the cells were found apoptotic or dead in treated HL60 cells compared to the untreated control.
Charge-4e superconductivity and chiral metal in the 45°-twisted bilayer cuprates and similar materials
Authors: Liu Yu-Bo , Zhou Jing , Wu Congjun , Yang Fan 
Published: June 2023

Abstract
The material realization of the charge-4e/6e superconductivity (SC) is a big challenge. Here we propose realization of the charge-4e SC and chiral metal in a class of materials made through stacking a homo-bilayer with the largest twist angle, dubbed as the twist-bilayer quasi-crystal (TB-QC), exampled by the 45\degree-twisted bilayer cuprates and 30\degree-twisted bilayer graphene. When each mononlayer hosts a pairing state with the largest pairing angular momentum, previous studies yield that the second-order interlayer Josephson coupling would drive chiral topological SC (TSC) in the TB-QC. Here we propose that, above the $T_c$ of the chiral TSC phase, either the total- or relative- pairing phase of the two layers can be unilateral quasi- ordered or ordered. In the form case, a Cooper pair from the top layer pairs with a Cooper pair from the bottom layer to form the charge-4e SC; in the latter case, a time-reversal symmetry breaking chiral metal phase is formed. Based on a thorough symmetry analysis, we arrive at the low-energy effective Hamiltonian describing the pairing- phase fluctuations. Our combined renormalization group and Monte-Carlo studies reveal the presence of the charge-4e SC and chiral metal phases in the phase diagram, characterized by various temperature-dependent quantities and spatial-dependent correlations.
Evaluation of the Mandibular Condyles Trabecular Structure in Patients with Severe Class III Pattern: A CT Fractal Analysis Study
Authors: Afzoon Saeed , Hasani Mahvash , Ghorbani Farhad 
Published: June 2023

Abstract
This study aimed to examine the morphological features of class III patients’ trabecular structure of the mandibular condyle in comparison with normal class I individuals. CT scans of 45 severe class III patients (age: 16–46) who were the candidates for orthogenetic surgery were selected and matched by age and sex with 45 normal class individuals. The trabecular bone structure of the left and right mandibular condyle in three CT planes (axial, sagittal, coronal) of the study group and control group were evaluated employing the fractal analysis. The result of the present study indicated that there were significant differences between the fractal dimensions (FD) values of class III patients and normal class I individuals in all three CT planes (axial, coronal, sagittal) (P < 0.001). There were no significant differences in FD values of males and females. The intra-group evaluation revealed that there was no correlation between age and FD values. No significant differences between the FD values of the left and right condyle were detected. The FD values of class III patients’ mandibular condyle were lower than those of typical class I individuals which indicated that these patients were more prone to DJD and TMD.
The application of pelvic binders in the pre-hospital setting for people with suspected pelvic fracture: a systematic review
Authors: Napoletano Antonello , Coclite Daniela , Latina Roberto , Fauci Alice Josephine , Biffi Annalisa , Castellini Greta , D’Angelo Daniela , Gianola Silvia , Iacorossi Laura , Porcu Gloria , Facchinetti Gabriella , Nitto Marco Di , Ruggieri Maria Pia , Coniglio Carlo , Fabbri Andrea , Iannone Primiano , Chiara Osvaldo , WG Italian National Institute of Health guideline Working Group 
Published: June 2023

Abstract
Background
The application of non-invasive pelvic circumferential compression devices (PCCDs), including pelvic binders, has shown to improve the position and stability of some types of pelvic fractures. To date the clinical efficacy of PCCDs application is still scarce and of poor quality. We assessed the clinical efficacy of pelvic binder pre-hospital in the management of pelvic fracture.
Methods
We systematically searched MEDLINE (PubMed), EMBASE and Cochrane CENTRAL up to June 2022. Two authors independently extracted data and appraised risk of bias using the Newcastle Ottawa Scale for observational studies. The quality of evidence was assessed using the Grading of Recommendations Assessment (GRADE) approach.
Results
We included ten studies with a total of 2770 participants. None of the included studies showed a statistically significant difference in overall mortality, 48h or 30-day mortality in PCCD group compared to no PCCD, whereas a reduction of mortality was observed in favour of the group with early PCCD when compared to PCCD placement after diagnosis (adjusted OR 0.00326; p = 0.039). Adjusted estimates did not show a clear indication of the benefits of PCCDs application vs no-PCCDs on the need for massive transfusion. Patients who received early PCCD vs PCCD after diagnosis had significantly less blood transfusion requirements (p = 0.009). The main reported complication was skin necrosis related to the comparison early PCCD vs late PCCD placement.
Conclusions
Our results suggest a positive effect of the early application of PCCDs on overall mortality, however, randomized controlled trials are needed in order to confirm preliminary results observed in observational studies.
Hypermethylation suppresses microRNA-219a-2 to activate the ALDH1L2/GSH/PAI-1 pathway for fibronectin degradation in renal fibrosis
Authors: Xiao Xiao , Huo Emily , Guo Chunyuan , Zhou Xiangjun , Hu Xiaoru , Dong Charles , Shi Huidong , Dong Zheng , Wei Qingqing 
Published: June 2023

Abstract
Epigenetic regulations, such as DNA methylation and microRNAs, play an important role in renal fibrosis. Here, we report the regulation of microRNA-219a-2 (mir-219a-2) by DNA methylation in fibrotic kidneys, unveiling the crosstalk between these epigenetic mechanisms. Through genome-wide DNA methylation analysis and pyro-sequencing, we detected the hypermethylation of mir-219a-2 in renal fibrosis induced by unilateral ureter obstruction (UUO) or renal ischemia/reperfusion, which was accompanied by a significant decrease in mir-219a-5p expression. Functionally, overexpression of mir-219a-2 enhanced fibronectin induction during hypoxia or TGF-β1 treatment of cultured renal cells. In mice, inhibition of mir-219a-5p suppressed fibronectin accumulation in UUO kidneys. ALDH1L2 was identified to be the direct target gene of mir-219a-5p in renal fibrosis. Mir-219a-5p suppressed ALDH1L2 expression in cultured renal cells, while inhibition of mir-219a-5p prevented the decrease of ALDH1L2 in UUO kidneys. Knockdown of ALDH1L2 enhanced PAI-1 induction during TGF-β1 treatment of renal cells, which was associated with fibronectin expression. In conclusion, the hypermethylation of mir-219a-2 in response to fibrotic stress attenuates mir-219a-5p expression and induces the up-regulation of its target gene ALDH1L2, which may reduce fibronectin deposition by suppressing PAI-1.
Quantum Designated Verifier Signature Scheme with Semi-Trusted Third-Party
Authors: Ling Zhang , Jia-hao Zhang , Xiang-jun Xin , Chao-yang Li 
Published: June 2023

Abstract

Designated verifier signature (DVS) schemes can be applied in many scenes, such as E-voting and E-bidding use DVS attributes to protect the secret of participants. Most traditional DVS schemes are vulnerable to quantum computer attacks. Then, an identity-based semi-trusted third-party quantum DVS (STQDVS) scheme is proposed, which is safe for quantum means of attack. In new STQDVS scheme, the third participant prepares entangled Bell states and allocates them to the signer and the designated verifier (DV). The signer uses the identity information as her public key and the measurement results of corresponding quantum sequences as her private key to generate the signature. The DV applies the identity information as his public key and the measurement results of corresponding quantum sequences as her private key to verify the signature. Non-transferability and hidden origin are guaranteed by the fact that the DV can generate the same signature as the signatory by means of a simulated signature step. Security analysis has shown that attacks such as forgery, inter-resending, and impersonation are ineffective for the proposed scheme and that even third-party cannot forge signatures. On the other hand, the whole process of the scheme does not require any quantum key distribution protocols, quantum one-way functions, and quantum state exchange tests, which makes the STQDVS scheme relatively simple and more efficient than similar schemes.
Molecular phylogeny of Lepidagathis sensu lato with notes on its biogeography and subgenus Lophostachys
Authors: Agarwala Kuldeep P. , Somani Shriti , Raveendran Bhagyasree , Surveswaran Siddharthan 
Published: June 2023

Abstract
Lepidagathis Willd., a genus belonging to the family Acanthaceae, is present pantropically and consists of about 151 species. Classification of Lepidagathis and Lophostachys has been under debate for a long period. Earlier, they were separated based on their distribution and morphology. However, according to Benoist 1911, the morphological differences were very slight; hence, both were considered under the genus Lepidagathis. Molecular analysis is the most effective method to clear up taxonomic issues, but more data is needed regarding Lepidagathis and Lophostachys. Lepidagathis cristata, the type species of this genus, has no molecular sequences available. Therefore, the purpose of this study was to sequence several plastid markers, which are frequently employed for the phylogeny of Acanthaceae and species DNA barcoding. Subsequently, phylogenetic trees using Bayesian and maximum likelihood bootstrap methods were built. The predominantly Asian species formed a clade which we characterize as Lepidagathis s.s was found monophyletic with strong Bayesian posterior probability support as well as good maximum-likelihood bootstrap support. Lepidagathis s.s was sister to another well-supported clade consisting of the genus Lophostachys. This phylogenetic study, along with the biogeographical distribution proves that Lepidagathis and Lophostachys are independently evolving clades, which can be treated as different subgenera.
Pneumatosis cystoides intestinalis is a special type of pneumatosis intestinalis with benign prognosis but needs to be distinguished from necrotizing pneumatosis intestinalis: a retrospective cohort study
Authors: Chen Shuang , Wang Chao , Cheng Jin , Wang Yi , Shen Zhan-Long , Bao Yu-Di , Xing Zhao-Dong , Bian Wen-Jie , Gao Zhi-Dong , Ye Ying-Jiang 
Published: June 2023

Abstract
Background
Pneumatosis cystoides intestinalis (PCI) is a special type of pneumatosis intestinalis (PI), however, its clinical features and prognosis distinguished from necrotizing PI (NPI) have not been elucidated sufficiently.
Methods
Clinical data of patients diagnosed with PCI and NPI from January 2011 to December 2020 in Peking University People's Hospital were reviewed. The clinical characteristics, treatment strategy, and prognosis of PCI and NPI were compared.
Results
A total of 25 patients diagnosed with PCI and 21 patients diagnosed with NPI were involved in this study. Patients in PCI group were accompanied by lighter inflammatory responses than NPI group. In the CT examination, patients in PCI group were observed with higher proportion of cystic gas (100.0% vs 0.0%, P < 0.001) and free peritoneal air (68.0% vs 14.3%, P < 0.001), and lower proportion of peritoneal irritation sign (0.0% vs 61.9%, P < 0.001), bowel effusion (0.0% vs 66.7%, P < 0.001) and ascites (0.0% vs 90.5%, P < 0.001) compared to NPI group. All patients in PCI group were cured after conservative treatment. However, 18 (85.7%) patients in NPI group had enterectomy, and 8 (38.1%) patients died in the hospital.
Conclusion
PCI is a special type of PI with benign prognosis. Early identification of PCI and differentiation from NPI is the prerequisite to avoid overtreatment.
Knowledge about diabetic retinopathy, Eye checkup services utilization and associated factors among adult diabetic patients at Public hospitals of Southeastern Ethiopia, 2022
Authors: Ahmed Abdulaziz Temam , Abduku Muhammadawwal , Aliyi Ahmednur Adem , Buta Anwar Tahir 
Published: June 2023

Abstract
Background
Every person with diabetes is at risk of diabetic retinopathy. Although, limited studies were conducted in Ethiopia and there are scarce and disagreement of evidence on knowledge about diabetic retinopathy and eye check up service utilization.
Objective
To assess knowledge of diabetic retinopathy, eye checkup services utilization, and associated factors among adult diabetic patients at public hospitals in southeastern Ethiopia in 2022.
Methods
A facility-based crossectional study was conducted among 422 systematically selected adult diabetic patients selected between 01 April and 01 May. Data collection instruments were adopted from previous studies. Data were entered into Epidata version 3 and exported to SPSS version 25 for analysis. Descriptive statistics and chart were used to summarize and present the results. Binary logistic regression analysis was used to identify factors associated with diabetic retinopathy knowledge and eye checkup services utilization. Accordingly, variables with a p-value < of 0.25 in the bivariate binary logistic regression were selected for multivariable logistic regression. In multivariable logistic regression, the adjusted odds ratio was calculated with 95% confidence intervals, and variables with a p-value < 0.05 were declared statistically significant factors.
Results
Of the 422 samples, 401 study participants participated with a 95% response rate. Two hundred and fifty-four (63.3%) [95%CI: 58.5, 68%] and 37% [95%CI: 32.5, 42%] of the participants had good knowledge of diabetic retinopathy and good uptake of eye services checkups respectively. Educational status of greater than 12th grade, possession of health insurance and good attitude towards diabetic retinopathy prevention were factors associated with good knowledge about diabetic retinopathy. In addition, urban residence, level of education of > grade 12, having health insurance, good knowledge of diabetic retinopathy, and good attitude towards prevention of diabetic retinopathy were significantly associated with good utilization of eye checkup services.
Conclusions
There is low knowledge, and utilization of eye checkup services towards diabetic retinopathy among diabetic patients when compared to the World Health Organization and other recommendations.
Evaluating High-Resolution Computed Tomography Derived 3-D Joint Space Metrics of the Metacarpophalangeal Joints Between Rheumatoid Arthritis and Age- and Sex-Matched Control Participants
Authors: Tse Justin J. , Contreras Dani , Salat Peter , Barber Claire E.H. , Hazlewood Glen S. , Barnabe Cheryl , Penney Chris , Ibrahem Ahmed , Mosher Dianne , Manske Sarah L. 
Published: June 2023

Abstract
Rheumatoid arthritis associated joint space narrowing is commonly evaluated through 2D X-ray radiographs. Unfortunately, changes and overlapping anatomy in smaller joints, such as those found within the hands, hinder conventional radiography. High resolution peripheral quantitative computed tomography (HR-pQCT), an un-paralleled in vivo X-ray-based imaging technique, provides 3D quantitative joint space metrics that may overcome limitations of 2D imaging. However, whether these metrics are sufficient for the differentiation between RA-associated joint changes and those influenced by age, sex, and obesity remains unknown. Therefore, we recruited a cohort of RA patients as well as age- and sex-matched healthy control participants and scanned their 2nd and 3rd metacarpophalangeal joints using HR-pQCT.
HR-pQCT-derived 3D joint space metrics (volume, width, standard deviation of width, maximum width, minimum width, and asymmetry) were not significantly different between RA and control groups (p > 0.05). This may be explained by the few RA participants with evidence of radiographic damage included in this study. Joint space volume, mean joint space width (JSW), maximum JSW, minimum JSW were larger in males than females (p < 0.05), while maximum JSW decreased with age. However, there were no significant association between joint space metrics and BMI. Thus, as individuals with RA are expected to have more joint space narrowing, further research is necessary to determine whether additional factors (e.g. co-morbidities) or novel 3D JSW metrics can aid in the detection of early signs of joint space.
Development, face and content validity of a preoperative functional capacity questionnaire – an observational study
Authors: Lauterbach Gerhard Paz , Lunardi Adriana Claudia , Carvalho Celso Ricardo Fernandes , Martins Mílton Arruda 
Published: June 2023

Abstract

Background
functional capacity, defined as the ability of an individual to perform aerobic work, has been shown to have prognostic value in preoperative risk assessment. Greater functional capacity predicts fewer perioperative complications, especially when measured through objective tests, such as the cardiopulmonary exercise test. Nonetheless, objective tests are not widely available, and only a few questionnaires are validated to estimate functional capacity in this setting. Our objective is to describe the development, face, and content validity of a preoperative functional capacity assessment questionnaire that helps identify patients capable of performing 4 or more METs of effort.
Method
This study followed the STROBE Statement guidance for observational studies. Patients undergoing preoperative risk assessment in a tertiary university hospital were submitted to open interviews about physical activities performed in the last month, and answers were compiled and organized in domains (commuting, household activities, efforts at work, leisure time activities) and estimated efforts in METs. The most frequent answers were used to create the first draft of the questionnaire along with the orientations on how to apply it. The questionnaire was presented to experts for adjustments and, finally, to patients undergoing preoperative risk assessment, leading to the creation of a final version.
Results
sixty patients undergoing preoperative risk assessment were submitted to the open interviews. The most frequent answers were about household activities or efforts during commuting. Only 22% of the patients reported being employed, but 92% of those also reported performing activities that required 4 or more METs. Nine physical therapists, one physician, one linguistic expert, and 10 patients were responsible for the face and content validity of the questionnaire. The final version comprised 24 questions: 8 about household activities, 3 about work activities, 5 about leisure time activities, and 8 about commuting.
Conclusions
The preoperative functional capacity questionnaire developed presents face and content validity for its use in the preoperative risk assessment and might increase the accuracy of the preoperative risk prediction.
Distinct αβ double-negative T cells discriminate between autoimmune lymphoproliferative syndrome and RAS-associated lymphoproliferative disease
Authors: Kurita Daisuke , Shiba Norio , Ohya Takashi , Murase Ayako , Shimosato Yuko , Yoshitomi Masahiro , Hattori Seira , Sasaki Koji , Nishimura Kenichi , Tsujimoto Shin-ichi , Takeuchi Masanobu , Tanoshima Reo , Kanegane Hirokazu , Kitagawa Norihiko , Ito Shuichi 
Published: June 2023

Abstract
Autoimmune lymphoproliferative syndrome (ALPS) is a disease of lymphocyte homeostasis caused by FAS-mediated apoptotic pathway dysfunction and is characterized by non-malignant lymphoproliferation with an increased number of TCRαβ+CD4−CD8− double-negative T cells (αβDNTs). Conversely, RAS-associated leuko-proliferative disease (RALD), which is caused by gain-of-functional somatic variants in KRAS or NRAS, is considered a group of diseases with a similar course. Herein, we present a 7-year-old Japanese female of RALD harboring NRAS variant that aggressively progressed to juvenile myelomonocytic leukemia (JMML) with increased αβDNTs. She eventually underwent hematopoietic cell transplantation due to acute respiratory distress which was caused by pulmonary infiltration of JMML blasts. In general, αβDNTs have been remarkably increased in ALPS; however, FAS pathway gene abnormalities were not observed in this case. This case with RALD had repeated shock/pre-shock episodes as the condition progressed. This shock was thought to be caused by the presence of a high number of αβDNTs. The αβDNTs observed in this case revealed high CCR4, CCR6, and CD45RO expressions, which were similar to Th17. These increased Th17-like αβDNTs have triggered the inflammation, resulting in the pathogenesis of shock, because Th17 secretes pro-inflammatory cytokines such as interleukin (IL)-17A and granulocyte-macrophage colony-stimulating factor. The presence of IL-17A-secreting αβDNTs has been reported in systemic lupus erythematosus (SLE) and Sjögren’s syndrome. The present case is complicated with SLE, suggesting the involvement of Th17-like αβDNTs in the disease pathogenesis. Examining the characteristics of αβDNTs in RALD, JMML, and ALPS may reveal the pathologies in these cases.