bluuebunny/crossref_metadata_2025_split huggingface dataset:
Intriguing Optical Properties due to the Intermolecular Interaction between AIE and ACQ Materials
Authors: Gong Zhichao , Rong Xuejiao , Sui Guomin , Jiang Xu , Xu Mengya , Wang Chenyu , Meng Shuxian 
Published: April 2023

AbstractTo take advantage of the respective strengths of aggregation‐induced emission (AIE) and aggregation‐caused quenching (ACQ) materials, in this paper a unique approach is taken which is that tetraphenylethene and triphenylamine (classical AIEs), borondipyrromethene and benzothiadiazole (classical ACQs) are selected to construct AIE@ACQ bimolecular systems by simple compounding method, focusing on the effects on optical properties such as fluorescence, photothermal therapy, phosphorescence, and photodynamic therapy. Above all, the mechanisms and laws of the system are preliminarily found. First, AIEs and ACQs must, respectively, have electron‐acceptor and electron‐donors to form an intermolecular push–pull structure to ensure that the two molecules are close to each other, and intermolecular π–π stacking interaction separates the highest occupied molecular orbitals and lowest unoccupied molecular orbitals to reduce ΔEst, causing intriguing optical properties. Second, the matching degree of the conjugate structure between AIEs and ACQs is also an important factor. Utilizing these systems, it is found that different structural compositions possess different optical synergy properties, so different AIE@ACQ bimolecular systems can be constructed according to the actual needs, which have the advantages of efficiency and convenience, providing a new idea for the united application of AIEs and ACQs.
Intracellular Adhesion Molecule‐1 Improves Responsiveness to Immune Checkpoint Inhibitor by Activating CD8+ T Cells
Authors: Lee Se‐Hoon , Kim Yeongmin , Jeon Bu‐Nam , Kim Gihyeon , Sohn Jinyoung , Yoon Youngmin , Kim Sujeong , Kim Yunjae , Kim Hyemin , Cha Hongui , Lee Na‐Eun , Yang Hyunsuk , Chung Joo‐Yeon , Jeong A‐Reum , Kim Yun Yeon , Kim Sang Gyun , Seo Yeonhee , Park Sehhoon , Jung Hyun Ae , Sun Jong‐Mu , Ahn Jin Seok , Ahn Myung‐Ju , Park Hansoo , Yoon Kyoung Wan 
Published: April 2023

AbstractImmune checkpoint inhibitor (ICI) clinically benefits cancer treatment. However, the ICI responses are only achieved in a subset of patients, and the underlying mechanisms of the limited response remain unclear. 160 patients with non‐small cell lung cancer treated with anti‐programmed cell death protein‐1 (anti‐PD‐1) or anti‐programmed death ligand‐1 (anti‐PD‐L1) are analyzed to understand the early determinants of response to ICI. It is observed that high levels of intracellular adhesion molecule‐1 (ICAM‐1) in tumors and plasma of patients are associated with prolonged survival. Further reverse translational studies using murine syngeneic tumor models reveal that soluble ICAM‐1 (sICAM‐1) is a key molecule that increases the efficacy of anti‐PD‐1 via activation of cytotoxic T cells. Moreover, chemokine (CXC motif) ligand 13 (CXCL13) in tumors and plasma is correlated with the level of ICAM‐1 and ICI efficacy, suggesting that CXCL13 might be involved in the ICAM‐1‐mediated anti‐tumor pathway. Using sICAM‐1 alone and in combination with anti‐PD‐1 enhances anti‐tumor efficacy in anti‐PD‐1‐responsive tumors in murine models. Notably, combinatorial therapy with sICAM‐1 and anti‐PD‐1 converts anti‐PD‐1‐resistant tumors to responsive ones in a preclinical study. These findings provide a new immunotherapeutic strategy for treating cancers using ICAM‐1.
Tumor‐Associated Macrophage‐Derived Exosomal LINC01232 Induces the Immune Escape in Glioma by Decreasing Surface MHC‐I Expression
Authors: Li Junjun , Wang Keshan , Yang Chao , Zhu Kai , Jiang Cheng , Wang Minjie , Zhou Zijie , Tang Nan , Wang Qiangping , Wang Siqi , Shu Pengwei , Yuan Hongliang , Xiong Zhiyong , Li Jinsong , Liang Tao , Rao Jin , Wang Xuan , Jiang Xiaobing 
Published: April 2023

AbstractTumor‐associated macrophage (TAM) infiltration facilitates glioma malignancy, but the underlying mechanisms remain unclear. Herein, it is reported that TAMs secrete exosomal LINC01232 to induce tumor immune escape. Mechanistically, LINC01232 is found to directly bind E2F2 and promote E2F2 entry into the nucleus; the two synergistically promots the transcription of NBR1. The increase in binding between NBR1 binding and the ubiquitinating MHC‐I protein through the ubiquitin domain causes an increase in the degradation of MHC‐I in autophagolysosomes and a decrease in the expression of MHC‐I on the surface of tumor cells, which in turn led to tumor cell escape from CD8+ CTL immune attack. Disruption of E2F2/NBR1/MHC‐I signaling with shRNAs or blockade with the corresponding antibodies largely abolishes the tumor‐supportive effects of LINC01232 and inhibits tumor growth driven by M2‐type macrophages. Importantly, knockdown of LINC01232 enhances the expression of MHC‐I on the surface of tumor cells and improves the response to reinfusion with CD8+ T cells. This study reveals the existence of critical molecular crosstalk between TAMs and glioma mediates through the LINC01232/E2F2/NBR1/MHC‐I axis to support malignant tumor growth, indicating that targeting this axis may have therapeutic potential.
Bone Lesion‐Derived Extracellular Vesicles Fuel Prometastatic Cascades in Hepatocellular Carcinoma by Transferring ALKBH5‐Targeting miR‐3190‐5p
Authors: Han Shenqi , Xue Lin , Wei Yi , Yong Tuying , Jia Wenlong , Qi Yongqiang , Luo Yiming , Liang Junnan , Wen Jingyuan , Bie Nana , Liang Huifang , Liu Qiumeng , Ding Zeyang , Yang Xiangliang , Gan Lu , Huang Zhao , Chen Xiaoping , Zhang Bixiang 
Published: April 2023

AbstractBone is the second leading metastatic site for hepatocellular carcinoma (HCC). Patients with HCC and bone metastasis suffer poor quality of life and reduced survival time. Extracellular vesicles (EVs) are widely involved in HCC formation and metastasis. However, the communication between primary HCC and bone lesions mediated by EVs remains unclear and the possible effect of bone metastasis on the progression of HCC remains largely unknown. Here, bone‐metastasized HCC‐derived EVs (BM‐EVs) are found to localize to orthotropic HCC cells and promote HCC progression. Mechanistically, miR‐3190‐5p (miR‐3190) is upregulated in intracellular HCC cells isolated from bone lesions as well as in their derived EVs. miR‐3190 in BM‐EVs is transferred into orthotopic tumor cells and enhances their metastatic capacity by downregulating AlkB homolog 5 (ALKBH5) expression. Decreased level of ALKBH5 exacerbates the prometastatic characteristics of HCC by modulating gene expression in N6‐methyladenosine‐dependent and ‐independent ways. Finally, antagomir‐miR‐3190‐loaded liposomes with HCC affinity successfully suppress HCC progression in mice treated with BM‐EVs. These findings reveal that BM‐EVs initiate prometastatic cascades in orthotopic HCC by transferring ALKBH5‐targeting miR‐3190 and miR‐3190 is serving as a promising therapeutic target for inhibiting the progression of HCC in patients with bone metastasis.
Demand analysis with structural changes: Model and application to the US blueberry market
Authors: Soto‐Caro Ariel , Wu Feng , Xia Tian , Guan Zhengfei 
Published: April 2023

AbstractThis study proposes a generalized demand system accommodating structural changes endogenously and examines how increasing imports affect blueberry prices and shipment values in the US market. Specifically, we integrate a smooth transformation function into the nested synthetic inverse demand system model (SIDS). We show that the proposed approach performs better than the standard SIDS in the context of structural changes in imports in the US blueberry market. As the largest blueberry producer in the world, the United States has supplied half of the world's blueberries over the past decade. Imported blueberries from South American countries do not have large impacts on the US blueberry industry due to the difference in production seasons. But the fast‐growing imports from Mexico have had great impacts on the US market. Our simulation shows that a 100% increase in Mexican blueberry shipments will result in a loss of $20 million in US domestic production. [EconLit Citations: Q11, Q17].
An inverse mapping approach for process systems engineering using automatic differentiation and the implicit function theorem
Authors: Alves Victor , Kitchin John R. , Lima Fernando V. 
Published: April 2023

AbstractThe objective in this work is to propose a novel approach for solving inverse problems from the output space to the input space using automatic differentiation coupled with the implicit function theorem and a path integration scheme. A common way of solving inverse problems in process systems engineering (PSE) and in science, technology, engineering and mathematics (STEM) in general is using nonlinear programming (NLP) tools, which may become computationally expensive when both the underlying process model complexity and dimensionality increase. The proposed approach takes advantage of recent advances in robust automatic differentiation packages to calculate the input space region by integration of governing differential equations of a given process. Such calculations are performed based on an initial starting point from the output space and are capable of maintaining accuracy and reducing computational time when compared to using NLP‐based approaches to obtain the inverse mapping. Two nonlinear case studies, namely a continuous stirred tank reactor (CSTR) and a membrane reactor for conversion of natural gas to value‐added chemicals are addressed using the proposed approach and compared against: (i) extensive (brute‐force) search for forward mapping and (ii) using NLP solvers for obtaining the inverse mapping. The obtained results show that the novel approach is in agreement with the typical approaches, while computational time and complexity are considerably reduced, indicating that a new direction for solving inverse problems is developed in this work.
Diazoalkenes: From an Elusive Intermediate to a Stable Substance Class in Organic Chemistry
Authors: Hansmann Max M. 
Published: April 2023

AbstractOver decades diazoalkenes (R2C=C=N2) were postulated as reactive intermediates in organic chemistry even though their direct spectroscopic detection proved very challenging. In the 1970/80ies several groups probed their existence mainly indirectly by trapping experiments or directly by matrix‐isolation studies. In 2021, our group and the Severin group reported independently the synthesis and characterization of the first room‐temperature stable diazoalkenes, which initiated a rapidly expanding research field. Up to now four different classes of N‐heterocyclic substituted room‐temperature stable diazoalkenes have been reported. Their properties and unique reactivity, such as N2/CO exchange or utilization as vinylidene precursors in organic and transition metal chemistry are presented. This review summarizes the early discoveries of diazoalkenes from their initial postulation as transient, elusive species up to the recent findings of the room‐temperature stable derivatives.
Mechanical properties of epoxy resin composites modified by epoxy styrene‐butadiene latex
Authors: Niu Chunmei , Du Kaipeng , Xu Zheng , Li Zhiqiang , Li Tiantian , Wang Ruolin 
Published: April 2023

AbstractTo improve toughness, crack resistance, and other mechanical properties of cured epoxy resins (EP), styrene‐butadiene rubbers (SBR) with epoxy groups named as ESBR were fabricated by emulsion polymerization and directly blending with EP in form of latex to make EP/rubber composites. Dispersity and compatibility of ESBR in epoxy resin were significantly improved. The results showed that impact strength for epoxy resin composite was up to 35.28 KJ/m2 when 8 phr ESBR with epoxy degree of 11% (ESBR‐11) was added, which was 127% higher than that of pure EP (15.18 KJ/m2). Toughness, bending strength, and tensile strength were simultaneously improved when the addition amount for ESBR‐11 was 2 phr. Compared with EP modified by SBR latex, butadiene vinyl‐pyridine rubber latex, carboxylate styrene‐butadiene latex and ESBR dissolved in chloroform, ESBR latex can significantly enhance mechanical properties of EP due to the uniform dispersion, good compatibility and strong chemical bonding between components.
Cell Type–Specific Biomarkers of Systemic Sclerosis Disease Severity Capture Cell‐Intrinsic and Cell‐Extrinsic Circuits
Authors: Berkowitz Jacob S. , Tabib Tracy , Xiao Hanxi , Sadej Gabrielle M. , Khanna Dinesh , Fuschiotti Patrizia , Lafyatis Robert A. , Das Jishnu 
Published: April 2023

ObjectiveSystemic sclerosis (SSc) is a multifactorial autoimmune fibrotic disorder involving complex rewiring of cell‐intrinsic and cell‐extrinsic signaling coexpression networks involving a range of cell types. However, the rewired circuits as well as corresponding cell–cell interactions remain poorly understood. To address this, we used a predictive machine learning framework to analyze single‐cell RNA‐sequencing data from 24 SSc patients across the severity spectrum as quantified by the modified Rodnan skin score (MRSS).MethodsWe used a least absolute shrinkage and selection operator (LASSO)–based predictive machine learning approach on the single‐cell RNA‐sequencing data set to identify predictive biomarkers of SSc severity, both across and within cell types. The use of L1 regularization helps prevent overfitting on high‐dimensional data. Correlation network analyses were coupled to the LASSO model to identify cell‐intrinsic and cell‐extrinsic co‐correlates of the identified biomarkers of SSc severity.ResultsWe found that the uncovered cell type–specific predictive biomarkers of MRSS included previously implicated genes in fibroblast and myeloid cell subsets (e.g., SFPR2+ fibroblasts and monocytes), as well as novel gene biomarkers of MRSS, especially in keratinocytes. Correlation network analyses revealed novel cross‐talk between immune pathways and implicated keratinocytes in addition to fibroblast and myeloid cells as key cell types involved in SSc pathogenesis. We then validated the uncovered association of key gene expression and protein markers in keratinocytes, KRT6A and S100A8, with SSc skin disease severity.ConclusionOur global systems analyses reveal previously uncharacterized cell‐intrinsic and cell‐extrinsic signaling coexpression networks underlying SSc severity that involve keratinocytes, myeloid cells, and fibroblasts.image
Cytosolic protein delivery using ultrasound‐guided vaporization of perfluorocarbon nano‐droplets
Authors: Yamaguchi Satoshi , Kisaka Miho , Higashi Kotaro , Ishijima Ayumu , Azuma Takashi , Nakagawa Keiichi , Shibasaki Yoshikazu , Sakuma Ichiro , Okamoto Akimitsu 
Published: April 2023

AbstractUltrasound‐guided protein delivery is promising for site‐specific control of cellular functions in the deep interior of the body in a noninvasive manner. Herein, we propose a method for cytosolic protein delivery based on ultrasound‐guided intracellular vaporization of perfluorocarbon nano‐droplets. The nano‐droplets were conjugated with cargo proteins through a bio‐reductively cleavable linker and introduced into living cells via antibody‐mediated binding to a cell‐surface receptor, which gets internalized through endocytosis. After the cells were exposed to ultrasound for endosomal escape of proteins, the ultrasound‐responsive cytosolic release of a cargo enzyme was confirmed by visualizing the hydrolysis of the fluorogenic substrate using confocal microscopy. Moreover, a significant decrease in cell viability was achieved via the release of a cytotoxic protein in response to ultrasound treatment. The results of this study provide the proof of a principle that protein‐conjugated nano‐droplets can be used as carriers in ultrasound‐guided cytosolic delivery of proteins.
Addressing amino acid‐derived inhibitory metabolites and enhancing CHO cell culture performance through DOE‐guided media modifications
Authors: Ladiwala Pranay , Dhara Venkata Gayatri , Jenkins Jackson , Kuang Bingyu , Hoang Duc , Yoon Seongkyu , Betenbaugh Michael J. 
Published: April 2023

AbstractPreviously, we identified six inhibitory metabolites (IMs) accumulating in Chinese hamster ovary (CHO) cultures using AMBIC 1.0 community reference medium that negatively impacted culture performance. The goal of the current study was to modify the medium to control IM accumulation through design of experiments (DOE). Initial over‐supplementation of precursor amino acids (AAs) by 100% to 200% in the culture medium revealed positive correlations between initial AA concentrations and IM levels. A screening design identified 5 AA targets, Lys, Ile, Trp, Leu, Arg, as key contributors to IMs. Response surface design analysis was used to reduce initial AA levels between 13% and 33%, and these were then evaluated in batch and fed‐batch cultures. Lowering AAs in basal and feed medium and reducing feed rate from 10% to 5% reduced inhibitory metabolites HICA and NAP by up to 50%, MSA by 30%, and CMP by 15%. These reductions were accompanied by a 13% to 40% improvement in peak viable cell densities and 7% to 50% enhancement in IgG production in batch and fed‐batch processes, respectively. This study demonstrates the value of tuning specific AA levels in reference basal and feed media using statistical design methodologies to lower problematic IMs.
Effects of SMC1A on immune microenvironment and cancer stem cells in colon adenocarcinoma
Authors: Li Jin , Zhou Qian , Liu Li , He Jingdong 
Published: April 2023

AbstractBackgroundOur previous study suggested that SMC1 has significant functions in colorectal cancer (CRC). However, few reports have shown the effects of structural maintenance of chromosomes 1 (SMC1A) on the immune microenvironment and tumor stem cells.MethodsThe Cancer Genome Atlas (TCGA) database, CPTAC database, Human Protein Atlas (HPA) database, the Cancer Cell Line Encyclopedia (CCLE) and Tumor Immune Single‐cell Hub were used. Flow cytometry and immunohistochemical analysis were checked for immune infiltration on MC38 mice model. Human CRC tissues were tested with RT‐qPCR.ResultsThe mRNA and protein levels of SMC1A were increased in colon adenocarcinoma (COAD) samples. SMC1A was associated with DNA activity. Interestingly, SMC1A was highly expressed in many types of immune cells at single‐cell levels. Moreover, the high expression of SMC1A was positively correlated with immune infiltration, and immunohistochemical analysis showed that SMC1A was positively associated with CD45 expression in MC38 mice model. Also, the percentage of IL4+CD4+ T cells (Th2) and FoxP3+CD4+ T cells (Tregs) was significantly higher in the SMC1A overexpression group than in control by flow cytometry assay in vivo. SMC1A expression could affect the proliferation of T cells in the mice model. The mutation and somatic cell copy number variation (SCNV) of SMC1A were also associated with immune cell infiltration. In addition to SMC1A in the “hot” T‐cell inflammatory microenvironment of colon cancer, SMC1A also positively correlates with the immune checkpoint genes CD274, CTLA4, and PDCD1 in colon adenocarcinoma (COAD) samples. Furthermore, we also found that SMC1A plays a positive correlation with the induction of cancer stem cells (CSCs). Our results also showed that miR‐23b‐3p binds SMC1A.ConclusionSMC1A may be a bidirectional target switch that simultaneously regulates the immune microenvironment and tumor stem cells. Moreover, SMC1A may be a biomarker for the prediction of immune checkpoint inhibitor (ICI) therapy.
Enhancing mental health and well‐being outcomes for psychotherapy and counselling through idiographic analysis: A four‐quadrant approach
Authors: Jacob Jenna , Rae John P. , Allegranti Beatrice , Duncan Charlie , Cooper Mick 
Published: April 2023

AbstractMental health and well‐being outcomes in psychotherapy and counselling have largely been studied through the use of standardised nomothetic measurement. A key limitation is that nomothetic measurement and current statistical analyses do not necessarily capture the outcomes of the complicated and individual psychotherapy processes. There is an increased interest in the use of idiographic patient‐reported outcome measures (I‐PROMs), which are uniquely useful because they may explore areas of importance that are unexplored by nomothetic outcome measures. We argue that to optimise the value of I‐PROMs, it is necessary to consider their contribution alongside standardised nomothetic measures. However, there are important considerations with respect to whether or not I‐PROMs from different clients, or indeed from the same client, can be meaningfully explored at a team/service level, and alongside standardised nomothetic outcomes. We provide worked examples on real client data to show that delineating four quadrants of analysis is important to explore the breadth of information: (a) individual progress on single items, (b) individual progress by aggregated scores, (c) team/service‐level progress by goal item/theme and (d) team/service‐level progress by aggregated scores.
Effects of payment notifications on consumer purchase decisions: The role of pain of payment
Authors: Gu Yu , Chen Rong 
Published: April 2023

AbstractPayment notifications are becoming ubiquitous due to the popularity of online payment, which are usually considered to be records of consumption. However, the effect of payment notifications on consumer behavior has not been fully understood. Given this research void, this study explores the effects of payment notifications on consumers' consumption amount during a multiple‐purchase shopping journey. Considering the prevalence of online payment in China, we conducted four experiments using Chinese samples. The results confirm that payment notifications can evoke consumers' pain of payment, which consequently reduces consumers' subsequent shopping amount. Additionally, we argue that the effects of payment notifications on consumer behavior are different in the early/late stage of the shopping journey or in the gift‐purchasing/self‐purchasing conditions. The findings of this article could help payment application developers to better design payment notification functions.
Subcritical Water Extraction of Phenolic Compounds from Vaccinium Dunalianum Wight Leaves and Their Antioxidant and Tyrosinase Inhibitory Activities in Vitro
Authors: Yang Xiaoqin , Wang Wenbo , Jiang Qian , Xie Sida , Zhao Ping , Liu Zhen , Zhu Guolei , Xu Junming , Wang Jieyu , Li Yonghe 
Published: April 2023

AbstractSubcritical water extraction was used to extract bioactive phenolic compounds from Vaccinium dunalianum Wight leaves. The optimal extraction conditions were determined as an extraction temperature of 150 °C, an extraction time of 40 min, and a liquid‐solid ratio of 35 : 1 mL/g. The total phenolic content reached 21.35 mg gallic acid /g, which was 16 % higher than that by hot water extraction. The subcritical water extraction extract exhibited strong scavenging activity of DPPH free radical and ABTS+ free radical, as well as significant tyrosinase inhibitory activity. The study suggests that subcritical water extraction can alter the composition of the extracts, leading to the production of various phenolic compounds, effective antioxidants, and tyrosinase inhibitors from Vaccinium dulciana Wight leaves. These findings confirm the potential of Vaccinium dunalianum Wight as a natural antioxidant molecule source for the medicine and food industries, and for the therapy of skin pigmentation disorders.