bluuebunny/crossref_metadata_2025_split huggingface dataset:
Contextualising Statelessness: Contingent Citizenship and The Politics of (non)Recognition in Thailand
Authors: Cheva-Isarakul Janepicha 
Published: September 2023

AbstractThe legal evidentiary approach to “solving” statelessness can sometimes lead to the issue being framed in terms of certain groups of people not meeting objective citizenship criteria or lacking required legal documents. Building on critical interdisciplinary scholarship in anthropology, history and legal studies, this article demonstrates the “constructedness” of citizenship and statelessness through the lens of the politics of recognition and documentation. Using Thailand as a case study, I highlight how global economic, political and social contexts play a significant and dynamic role in delineating the legal line of membership. By tracing how Thai nationality has been instrumentalised by the state throughout the twentieth century, this article contextualises statelessness as a legal and social by-product of statemaking. As such, it challenges the framing of nationality as a non-discriminatory mode of recognition founded in legal objectivity and reiterates the politics of statelessness. In emphasising the fragility of citizenship when granted without genuine social, political and moral recognition, I argue that the objective of statelessness advocacy should not simply be about turning stateless persons into citizens, but rather about creating a more equitable society wherein one's rights are upheld regardless of legal status.
Effect of spray droplet spectra on control of Poa annua with pronamide
Authors: Ignes Martin , Ferguson J. Connor , Tseng Te-Ming , Stewart Barry R. , Castro Edicarlos B. , McCurdy James D. 
Published: September 2023

AbstractAnnual bluegrass is a troublesome weed in turfgrass, with reported resistance to at least 12 herbicide sites of action. The mitotic-inhibiting herbicide pronamide has both preemergence and postemergence activity on susceptible annual bluegrass populations. Previous studies suggest that postemergence activity may be compromised due to lack of root uptake, as well as target-site- and translocation-based mechanisms. Research was conducted to determine the effects of spray droplet spectra on spray coverage and control of annual bluegrass with pronamide, flazasulfuron, and a mixture of pronamide plus flazasulfuron. Herbicides were delivered to annual bluegrass plants having two to three leaves via five different spray spectra based on volume median diameters (VMD) of 200, 400, 600, 800, and 1,000 µm. Fluorescent tracer dye was added to each treatment solution to quantify the effects of herbicide and spray droplet spectra on herbicide deposition. In another experiment, the efficacy of 0.58, 1.16, and 2.32 kg pronamide ha−1; 0.022, 0.044, and 0.088 kg flazasulfuron ha−1, or a combination of the two, were assessed in iteration with droplet spectrum sprays of 400 and 1,000 µm on two pronamide-resistant and two pronamide-susceptible annual bluegrass populations. Spray droplet spectrum affected the deposition of pronamide and flazasulfuron, applied alone and in combination. Pronamide foliar deposition decreased with increasing droplet spectra. Pronamide efficacy was affected by droplet spectrum, with the largest (1,000 µm) exhibiting improved control. Flazasulfuron efficacy and pronamide plus flazasulfuron efficacy were not affected by droplet spectra. Pronamide plus flazasulfuron mixture controlled all four populations more effectively than pronamide alone, regardless of droplet spectra. A mixture of pronamide plus flazasulfuron applied with relatively large droplets may be optimal for annual bluegrass control, which offers valuable insights for optimizing herbicide application and combatting herbicide resistance. However, applications in this controlled-growth pot study may not mimic conditions in which thatch and turfgrass canopy limit the soil deposition of pronamide.
Evaluation of crop-topping strategies to reduce common lambsquarters (Chenopodium album) seed production in potato production systems
Authors: Anderson Laura , White Scott Neil , McKenzie-Gopsill Andrew 
Published: September 2023

AbstractPotato producers in Canada’s Atlantic provinces of Prince Edward Island (PE) and New Brunswick rely on photosystem II (PSII)-inhibiting herbicides to provide season-long weed control. Despite this fact, a high proportion of common lambsquarters populations in the region have been identified as resistant to this class of herbicides. Crop-topping is a late-season weed management practice that exploits the height differential between weeds and a developing crop canopy. Two field experiments were conducted in Harrington, PE, in 2020 and 2021, one each to evaluate the efficacy of a different crop-topping strategy, above-canopy mowing or wick-applied glyphosate, at two potato phenological stages, on common lambsquarters viable seed production and potato yield and quality. Mowing common lambsquarters postflowering decreased viable seed production (72% to 91%) in 2020 but increased seed production (78% to 278%) in 2021. Mowing had minimal impact on potato marketable yield across cultivars in both years. In contrast, treating common lambsquarters with wick-applied glyphosate had variable impacts on seed output in 2020 but dramatically reduced seed production (up to 95%) in 2021 when treatments were applied preflowering. Glyphosate damage to potato tubers was not influenced by timing and resulted in a 14% to 15% increase in culled tubers due to black spotting and rot. Our results highlight the importance of potato and common lambsquarters phenology when selecting a crop-topping strategy and demonstrate that above-canopy mowing and wick-applied glyphosate can be utilized for seedbank management of herbicide-resistant common lambsquarters in potato production systems.
Weed control in corn with tolpyralate and atrazine plus grass herbicides
Authors: Soltani Nader , Shropshire Christy , Sikkema Peter H. 
Published: September 2023

AbstractSix field experiments were established in southwestern Ontario in 2021 and 2022 to evaluate whether the addition of a grass herbicide (acetochlor, dimethenamid-p, flufenacet, pendimethalin, pyroxasulfone, or S-metolachlor) to tolpyralate + atrazine improves late-season weed control in corn. Tolpyralate + atrazine caused 12% and 5% corn injury at 1 and 4 wk after herbicide application (WAA); corn injury was not increased with the addition of a grass herbicide. Weed interference reduced corn yield 60%. The addition of a grass herbicide to tolpyralate + atrazine did not enhance velvetleaf control. The addition of acetochlor or dimethenamid-p to tolpyralate + atrazine enhanced pigweed species control 4% 4 WAA; the addition of other grass herbicides tested did not increase pigweed species control. The addition of acetochlor enhanced common ragweed control 5% at 4 WAA, and the addition of acetochlor or dimethenamid-p enhanced common ragweed control 8% at 8 WAA; the addition of other grass herbicides did not improve common ragweed control. The addition of acetochlor to tolpyralate + atrazine enhanced common lambsquarters control up to 4%; there was no enhancement in common lambsquarters control with the addition of the other grass herbicides. Tolpyralate + atrazine controlled barnyardgrass 90% and 78% at 4 and 8 WAA, respectively; the addition of a grass herbicide enhanced barnyardgrass control 9% to 10% and 21% at 4 and 8 WAA, respectively. Tolpyralate + atrazine controlled green or giant foxtail 80% and 69% at 4 and 8 WAA, respectively; the addition of a grass herbicide enhanced foxtail species control 15% to 19% and 24% to 29% at 4 and 8 WAA, respectively. This research shows that adding a grass herbicide to tolpyralate + atrazine mixture can improve weed control efficacy, especially increased annual grass control in corn production.
A global perspective of education in weed science
Authors: Ahmad Taseer , Jabran Khawar , Cheema Zahid Ata , Bajwa Ali Ahsan , Farooq Muhammad 
Published: September 2023

AbstractIn modern agriculture, weed problems are predicted to worsen and become more complicated as a result of increasing invasiveness, herbicide resistance, and emphasis on high-input methods. Weeds cause huge economic yield losses that range from US$100 million to US$26 billion globally. The knowledge of weed science has offered success in the past through effective, reasonably priced, and secure technologies; specifically, synthetic herbicides to effectively control weeds in agroecosystems. Weed science is accepted and adopted by many universities with teaching, research, and/or extension programs in agriculture. Globally, approximately 7% of all the universities offering agricultural education have dedicated weed science departments focusing on weed biology, ecology, and management. Some universities also offer weed science degree programs or at least certain courses in their degrees related to associated disciplines, such as plant protection, agronomy, and ecology. Although substantial advances have been made in weed science, such as a separate weed science discipline, specialized journals, and specific weed science societies and conferences worldwide, many constraints (e.g., lack of trained weed scientists) and barriers to adoption of new weed science technologies remain. Slow modernization in weed science research and low funding has slowed the progress of this discipline. New curricula in the weed science discipline should focus on the role of biochemistry, evolutionary biology, molecular biology, and genetics in weed science research.
Clinical, morphologic and molecular heterogeneity of HPV-associated oropharyngeal cancer
Authors: Lim Yvonne X. , Mierzwa Michelle L. , Sartor Maureen A. , D’Silva Nisha J. 
Published: September 2023

AbstractThe incidence of human papillomavirus-positive (HPV+) oropharyngeal squamous cell carcinoma (OPSCC) is rising rapidly and has exceeded cervical cancer to become the most common HPV-induced cancer in developed countries. Since patients with HPV + OPSCC respond very favorably to standard aggressive treatment, the emphasis has changed to reducing treatment intensity. However, recent multi-center clinical trials failed to show non-inferiority of de-escalation strategies on a population basis, highlighting the need to select low-risk patients likely to respond to de-intensified treatments. In contrast, there is a substantial proportion of patients who develop recurrent disease despite aggressive therapy. This supports that HPV + OPSCC is not a homogeneous disease, but comprises distinct subtypes with clinical and biological variations. The overall goal for this review is to identify biomarkers for HPV + OPSCC that may be relevant for patient stratification for personalized treatment. We discuss HPV + OPSCC as a heterogeneous disease from multifaceted perspectives including clinical behavior, tumor morphology, and molecular phenotype. Molecular profiling from bulk tumors as well as single-cell sequencing data are discussed as potential driving factors of heterogeneity between tumor subgroups. Finally, we evaluate key challenges that may impede in-depth investigations of HPV + OPSCC heterogeneity and outline potential future directions, including a section on racial and ethnic differences.
Biological differences underlying sex and gender disparities in bladder cancer: current synopsis and future directions
Authors: Doshi Bhavisha , Athans Sarah R. , Woloszynska Anna 
Published: September 2023

AbstractSex and gender disparities in bladder cancer have long been a subject of interest to the cancer research community, wherein men have a 4 times higher incidence rate than women, and female patients often present with higher-grade disease and experience worse outcomes. Despite the known differences in disease incidence and clinical outcomes between male and female bladder cancer patients, clinical management remains the same. In this review, we critically analyze studies that report on the biological differences between men and women and evaluate how these differences contribute to sex and gender disparities in bladder cancer. Distinct characteristics of the male and female immune systems, differences in circulating hormone levels and hormone receptor expression, and different genetic and epigenetic alterations are major biological factors that all likely contribute to disparate incidence rates and outcomes for male and female bladder cancer patients. Future preclinical and clinical studies in this area should employ experimental approaches that account for and consider sex and gender disparities in bladder cancer, thereby facilitating the development of precision medicine for the effective treatment of bladder cancer in all patients.
Generation of inactivated IL2RG and RAG1 monkeys with severe combined immunodeficiency using base editing
Authors: Zheng Xiao , Huang Chunhui , Lin Yingqi , Han Bofeng , Chen Yizhi , Li Caijuan , Li Jiawei , Ding Yongyan , Song Xichen , Wang Wei , Liang Weien , Wu Jianhao , Wu Jiaxi , Gao Jiale , Wei Chengxi , Zhang Xudong , Tu Zhuchi , Yan Sen 
Published: September 2023

AbstractSevere combined immunodeficiency (SCID) encompasses a range of inherited disorders that lead to a profound deterioration of the immune system. Among the pivotal genes associated with SCID, RAG1 and IL2RG play crucial roles. IL2RG is essential for the development, differentiation, and functioning of T, B, and NK cells, while RAG1 critically contributes to adaptive immunity by facilitating V(D)J recombination during the maturation of lymphocytes. Animal models carrying mutations in these genes exhibit notable deficiencies in their immune systems. Non-human primates (NHPs) are exceptionally well-suited models for biomedical research due to their genetic and physiological similarities to humans. Cytosine base editors (CBEs) serve as powerful tools for precisely and effectively modifying single-base mutations in the genome. Their successful implementation has been demonstrated in human cells, mice, and crop species. This study outlines the creation of an immunodeficient monkey model by deactivating both the IL2RG and RAG1 genes using the CBE4max system. The base-edited monkeys exhibited a severely compromised immune system characterized by lymphopenia, atrophy of lymphoid organs, and a deficiency of mature T cells. Furthermore, these base-edited monkeys were capable of hosting and supporting the growth of human breast cancer cells, leading to tumor formation. In summary, we have successfully developed an immunodeficient monkey model with the ability to foster tumor growth using the CBE4max system. These immunodeficiency monkeys show tremendous potential as valuable tools for advancing biomedical and translational research.
Functional conservation of microbial communities determines composition predictability in anaerobic digestion
Authors: Lin Qiang , Li Lingjuan , De Vrieze Jo , Li Chaonan , Fang Xiaoyu , Li Xiangzhen 
Published: September 2023

AbstractA major challenge in managing and engineering microbial communities is determining whether and how microbial community responses to environmental alterations can be predicted and explained, especially in microorganism-driven systems. We addressed this challenge by monitoring microbial community responses to the periodic addition of the same feedstock throughout anaerobic digestion, a typical microorganism-driven system where microorganisms degrade and transform the feedstock. The immediate and delayed response consortia were assemblages of microorganisms whose abundances significantly increased on the first or third day after feedstock addition. The immediate response consortia were more predictable than the delayed response consortia and showed a reproducible and predictable order-level composition across multiple feedstock additions. These results stood in both present (16 S rRNA gene) and potentially active (16 S rRNA) microbial communities and in different feedstocks with different biodegradability and were validated by simulation modeling. Despite substantial species variability, the immediate response consortia aligned well with the reproducible CH4 production, which was attributed to the conservation of expressed functions by the response consortia throughout anaerobic digestion, based on metatranscriptomic data analyses. The high species variability might be attributed to intraspecific competition and contribute to biodiversity maintenance and functional redundancy. Our results demonstrate reproducible and predictable microbial community responses and their importance in stabilizing system functions.
Ectopic callose deposition into woody biomass modulates the nano-architecture of macrofibrils
Authors: Bourdon Matthieu , Lyczakowski Jan J. , Cresswell Rosalie , Amsbury Sam , Vilaplana Francisco , Le Guen Marie-Joo , Follain Nadège , Wightman Raymond , Su Chang , Alatorre-Cobos Fulgencio , Ritter Maximilian , Liszka Aleksandra , Terrett Oliver M. , Yadav Shri Ram , Vatén Anne , Nieminen Kaisa , Eswaran Gugan , Alonso-Serra Juan , Müller Karin H. , Iuga Dinu , Miskolczi Pal Csaba , Kalmbach Lothar , Otero Sofia , Mähönen Ari Pekka , Bhalerao Rishikesh , Bulone Vincent , Mansfield Shawn D. , Hill Stefan , Burgert Ingo , Beaugrand Johnny , Benitez-Alfonso Yoselin , Dupree Ray , Dupree Paul , Helariutta Ykä 
Published: September 2023

AbstractPlant biomass plays an increasingly important role in the circular bioeconomy, replacing non-renewable fossil resources. Genetic engineering of this lignocellulosic biomass could benefit biorefinery transformation chains by lowering economic and technological barriers to industrial processing. However, previous efforts have mostly targeted the major constituents of woody biomass: cellulose, hemicellulose and lignin. Here we report the engineering of wood structure through the introduction of callose, a polysaccharide novel to most secondary cell walls. Our multiscale analysis of genetically engineered poplar trees shows that callose deposition modulates cell wall porosity, water and lignin contents and increases the lignin–cellulose distance, ultimately resulting in substantially decreased biomass recalcitrance. We provide a model of the wood cell wall nano-architecture engineered to accommodate the hydrated callose inclusions. Ectopic polymer introduction into biomass manifests in new physico-chemical properties and offers new avenues when considering lignocellulose engineering.
Enhancing ultrafiltration membrane permeability and antifouling performance through surface patterning with features resembling feed spacers
Authors: Ibrahim Yazan , Hilal Nidal 
Published: September 2023

AbstractThis study investigates the potential of surface-patterned membranes in replacing the conventional feed spacer-assisted filtration process. Two types of surface patterns, namely diamond (D) and honeycomb (HC), were utilized in the fabrication of the surface-patterned membranes. These membranes showed larger effective membrane area and higher average porosity when compared to the flat PES membrane. The D-Patterned and HC-Patterned membranes achieved pure water flux of 76.6 ± 16.9 and 88.3 ± 17.4 L m−2 h−1, respectively, which was higher than the flat PES membrane with and without the use of feed spacers. Furthermore, the patterned membranes reported lower natural organic matter (NOM) fouling in short and long-term filtration experiments and higher flux recovery ratio (FRR), indicating their superior anti-fouling performance over the PES membrane when using feed spacers. Overall, the simple fabrication technique, higher permeability, and excellent anti-fouling performance of the surface-patterned membranes render them suitable for potentially replacing the current configuration used in spacer-assisted filtration processes.
Precisely patterned nanofibres made from extendable protein multiplexes
Authors: Bethel Neville P. , Borst Andrew J. , Parmeggiani Fabio , Bick Matthew J. , Brunette TJ , Nguyen Hannah , Kang Alex , Bera Asim K. , Carter Lauren , Miranda Marcos C. , Kibler Ryan D. , Lamb Mila , Li Xinting , Sankaran Banumathi , Baker David 
Published: September 2023

AbstractMolecular systems with coincident cyclic and superhelical symmetry axes have considerable advantages for materials design as they can be readily lengthened or shortened by changing the length of the constituent monomers. Among proteins, alpha-helical coiled coils have such symmetric, extendable architectures, but are limited by the relatively fixed geometry and flexibility of the helical protomers. Here we describe a systematic approach to generating modular and rigid repeat protein oligomers with coincident C2 to C8 and superhelical symmetry axes that can be readily extended by repeat propagation. From these building blocks, we demonstrate that a wide range of unbounded fibres can be systematically designed by introducing hydrophilic surface patches that force staggering of the monomers; the geometry of such fibres can be precisely tuned by varying the number of repeat units in the monomer and the placement of the hydrophilic patches.
Comparative single-cell transcriptomic analysis of primate brains highlights human-specific regulatory evolution
Authors: Suresh Hamsini , Crow Megan , Jorstad Nikolas , Hodge Rebecca , Lein Ed , Dobin Alexander , Bakken Trygve , Gillis Jesse 
Published: September 2023

AbstractEnhanced cognitive function in humans is hypothesized to result from cortical expansion and increased cellular diversity. However, the mechanisms that drive these phenotypic innovations remain poorly understood, in part because of the lack of high-quality cellular resolution data in human and non-human primates. Here, we take advantage of single-cell expression data from the middle temporal gyrus of five primates (human, chimp, gorilla, macaque and marmoset) to identify 57 homologous cell types and generate cell type-specific gene co-expression networks for comparative analysis. Although orthologue expression patterns are generally well conserved, we find 24% of genes with extensive differences between human and non-human primates (3,383 out of 14,131), which are also associated with multiple brain disorders. To assess the functional significance of gene expression differences in an evolutionary context, we evaluate changes in network connectivity across meta-analytic co-expression networks from 19 animals. We find that a subset of these genes has deeply conserved co-expression across all non-human animals, and strongly divergent co-expression relationships in humans (139 out of 3,383, <1% of primate orthologues). Genes with human-specific cellular expression and co-expression profiles (such as NHEJ1, GTF2H2, C2 and BBS5) typically evolve under relaxed selective constraints and may drive rapid evolutionary change in brain function.