bluuebunny/crossref_metadata_2025_split huggingface dataset:
Management of Proptosis in a Case of Sphenoid-Orbital Meningioma
Authors: Rahman Md Moshiur , Khan Robert Ahmed , Khan SIM Khairun Nabi , Arifin Mohammad Samsul , Chowdhury Muhtamim 
Published: September 2023

Sphenoid-orbital meningiomas (SOMs) are difficult to completely resect because they involve the sphenoid wing, orbit, and cavernous sinus. As the tumor invades the optic canal, SOMs frequently present with visual deficits. The authors of this case report dealt with the case of a 65-year-old male patient who had right-sided proptosis. We diagnosed the patient, and his MRI revealed of him having SOM. The surgical approach was determined by the tumor's placement in the orbit and cranial cavity and its connections to the optic canal and optic nerve axis. Tumor was removed successfully by surgery. The patient retained a proptosis of less than 4 mm during the follow-up period. The visual acuity, visual field, and ocular fundus were examined during the follow-up. SOMs are notoriously difficult to treat surgically. The primary goal of surgery in symptomatic individuals with SOMs is to slow down the tumor's progression. Longer periods of post-operative follow-up is recommended to observe long-term results.
Bang. J Neurosurgery 2022; 12(1): 65-68
Standardization of the Pharmaceutical Substance of the Silicon-zinc-boron-containing Glycerohydrogel
Authors: Permikin V. V. , Nikitina E. Yu. , Shadrina E. V. , Khonina T. G. 
Published: September 2023

Introduction. Silicon-zinc-boron-containing glycerohydrogel substance is considered as a pharmaceutical substance for the new drugs creation with antimicrobial action for local, external and intravaginal use. Standardization and validation of the methods determining the quality parameters of the corresponding substances is one of the stages of introducing new pharmacologically active compounds into practice.Aim. Determine the quality parameters of silicon-zinc-boron-containing glycerohydrogel, conduct their approbation. Develop a method for quantitative determination of silicon, zinc and boron. Perform statistical results processing of quantitative determination and provide a validation assessment of the chosen method.Materials and methods. A silicon-zinc-boron-containing glycerohydrogel substance was synthesized at the Institute of Organic Synthesis of the Ural Branch of the Russian Academy of Sciences and was used as the studying object. The studies were performed using a Nicolet 6700 (Thermo Fisher Scientific, USA) spectrometer and an OPTIMA 4300 DV (PerkinElmer, США) optical emission spectrometer with inductively coupled plasma.Results and discussion. The paper describes the results of new pharmacologically active silicon-zinc-boron-containing glycerohydrogel substance qualitative and quantitative tests. The data of statistical processing and validation assessment of the quantitative determination of silicon, zinc, and boron are presented.Conclusion. The quality parameters of silicon-zinc-boron-containing glycerohydrogel were determined. A method of silicon, zinc and boron quantitative determination was developed. Standardization of the investigated pharmacologically active substance was carried out in accordance with the current requirements for pharmaceutical substances in the Russian Federation.
Antibacterial Activity of Essential Oils of <i>Thymus serpyllum</i> L. and <i>Thymus marschallianus</i> Willd.
Authors: Shapoval O. G. , Sheremetyeva A. S. , Durnova N. A. , Mukhamadiev N. Q. , Rabbimova G. T. , Nazirbekov M. K. , Kupryashina M. A. 
Published: September 2023

Introduction. Plant essential oils are supposed to be applied for local treatment of infectious focuses.Aim. The aim of this study was evaluation of antimicrobial activity of essential oils of Thymus serpyllum L. and Thymus marschallianus Willd., widely distributed in the Saratov region.Materials and methods. Antimicrobial activity of the essential oils was determined by a serial dilution test at the microbial number of 5 × 105 CFU/ml against 6 strains of Staphylococcus aureus, including S. aureus FDA 209P and five clinical, 2 strains of Escherichia coli – E. coli ATCC 25922 and one clinical, two strains of Pseudomonas aeruginosa – P. aeruginosa ATCC 27835 and one clinical.Results and discussion. The bactericidal minimal inhibitory concentrations (MICs) of essential oil of T. serpyllum were determined for all strains of staphylococci (for 5 – 1097,5, for 1 – 2195 µg/ml). The bacteriostatic MICs were determined for 3 strains (for 2 – 548,75, for 1 – 1097,5 µg/ml). MICs of the T. marschallianus essential oil were bactericidal for all strains of S. aureus (120 for 5 and 480 µg/ml for 1 strain). For all test strains of gram-negative bacteria MICs of the essential oils of both species were bactericidal and amounted to 1097 and 960 µg/ml. Considering the chemical composition of the oils by thymol and its isomers, MIC50 of T. marschallianus and T. serpyllum for the test staphylococcal strains amounted 108,89 and 496,59, for the test strains of gram-negative bacteria 683,91 and 783,43 µg/ml respectively.Conclusion. According to the values of MIC50 antistaphylococcal activity of the essential oils of T. marschallianus was significantly higher than this activity of the essential oils of T. serpyllum.
Methodological Approaches to Risk Management of Advanced Therapy Medicinal Products
Authors: Taube A. A. , Velts N. Yu. 
Published: September 2023

Introduction. Advanced therapy medicinal products (ATMPs) rely on recent advances in medical science, but alongside with potential benefits they may also bring safety concerns for patients. The inherent complexity of the ATMP production and use calls for special approaches to risk management throughout their lifecycle, from obtaining the raw materials to administration to the patient.Aim. The aim of the present study was to develop approaches to risk management for ATMPs, using the example of CAR T-cell therapy.Materials and methods. The study analysed the relevant regulatory frameworks currently in force in the European Union and the United States of America, namely the regulations and guidelines adopted by the Center for Biologics Evaluation and Research of the U.S. Food and Drug Administration and the Committee for Medicinal Products for Human Use of the European Medicines Agency.Results and discussion. The paper provides a classification of patient risks, which was developed based on the European risk-based approach. It formulates the principles of risk management for each of the risks, depending on the stage of the product life cycle. Each type of risk was considered separately. The following risk minimization strategies were determined: compliance with the good practices, ensuring the necessary qualifications or expertise of all parties involved in the product life cycle. The main element of risk control is the detailed description of the medicinal product use in the summary of product characteristics and patient information leaflet.Conclusion. The study identified the main stages at which ATMP risks may occur, and each type of risk was considered separately. The following requirements should be put in place in order to manage the ATMP risks: requirements for distributors on how to perform the product transportation and storage and to keep records for the marketing authorisation holder; requirements for healthcare facilities on how to perform the product storage, its preparation for use, advising and informing the patients on the treatment risks, symptoms of adverse events, preparatory and follow-up medical procedures, and on how to keep records for the marketing authorisation holder; requirements for the qualifications of healthcare professionals who are in charge of the product storage, its preparation for use, treatment procedures, advising and informing the patients on the treatment risks, symptoms of adverse events, and follow-up medical procedures. The data obtained will be used in the preparation of recommendations for ATMP developers.
DIRECTED READING-THINKING ACTIVITY (DRTA) STRATEGY IN READING CLASS AT SMA NEGERI 2 KOTA TERNATE
Authors: Lambutu Safitri , Jusnita Naniek , Bahara Susana R. 
Published: September 2023

This study aims to determine whether the Directed Reading Thinking Activity (DRTA) strategy can improve students' reading comprehension at SMA Negeri 2 Kota Ternate. The research method used is pre-experimental design. Which consists of pre-test, treatment, and post-test design. The population in this study were students of class X-G SMA Negeri 2 Kota Ternate. Using a convenience sampling technique involving one X-G class. as the sample, 30 students at class. The results of the study show that the Directed Reading Thinking Activity (DRTA) strategy can improve students' reading comprehension in Class X-G at SMA Negeri 2 Kota Ternate. It is proven that the Pre-Test score is 48.73 and the Post-Test value is 68.77. Based on the difference in scores obtained by students in reading comprehension, it showed an increase from the pre-test to the post-test where the increase in students' reading comprehension increased. Significant improvement in student achievement. Thus, the null hypothesis (H0) is not accepted, while the alternative hypothesis (Hi) is accepted. Therefore the researcher concluded that. The Directed Reading Thinking Activity (DRTA) strategy is effective in learning reading comprehension. because students understand more easily with predictions that identify main ideas and supporting details and can be seen from the results of better student reading scores.
An alternative method to measure electrical conductivity (EC) and sodium adsorption ratio (SAR) in salt-affected soil extracts
Authors: Álvarez Torres Beverly , Sotomayor Ramírez David R. , Castro Chacón José Pablo , Martínez Rodríguez Gustavo , Pérez Alegría Luis R. , DeSutter Thomas M. 
Published: September 2023

Soil degradation due to salts affects over 100 countries, especially in arid and semi-arid regions where salts migrate to the plant root zone via capillary action when evapotranspiration exceeds rainfall. Soil salinity reduces germination, growth, and root development, impacting crop yields, while excess sodium decreases water movement into the soil. Soil properties, namely, electrical conductivity (ECe), sodium adsorption ratio (SARe), and pH (pHe), affected by sparingly and soluble salts, are typically analyzed using soil saturated paste (SP). However, a simpler and cost-effective alternative is assessing soil salinity using soil:water solutions at ratio 1:5 (SW). This study developed empirical models between EC1:5-ECe, SAR1:5-SARe, and pH1:5-pHe to monitor soil salinity and sodicity in Lajas Valley, Puerto Rico, an agricultural reserve with 1,140 mm of mean annual rainfall and soils classified as saline and/or sodic. The ECe Sampling, Assessment, and Prediction software for Response Surface Sampling Design (ESAP-RSSD) optimized soil sampling with 48 points. Measurements of EC, pH, cations (Ca2+, Mg2+, Na+), and SAR were conducted using SP and 1:5 SW extracts. Simple linear regression models estimated ECe (R2 &gt; 0.93, p &lt; 0.0001) and SARe (R2 &gt; 0.98, p &lt; 0.0001) from 1:5 extracts. The pHe models varied with depth, showing a strong correlation (R2 &gt; 0.62, p &lt; 0.0001) from 0 to 30 cm and weakening (R2 &gt; 0.27, p &lt; 0.0022) from 90 to 120 cm. The simple linear regression models generally perform well for EC and pH variables, with better performance observed at shallower depths. SW proves to be a practical, cost-effective, and efficient method for assessing salt-affected soils in Lajas Valley. By enabling regular soil salinity analysis, the developed estimation models combined with SW extraction could improve soil management practices and agricultural productivity.
Landslide displacement prediction from on-site deformation data based on time series ARIMA model
Authors: Wang Zhao , Tang Jiakui , Hou Shengshan , Wang Yanjiao , Zhang Anan , Wang Jiru , Wang Wuhua , Feng Zhen , Li Ang , Han Bing 
Published: September 2023

Time series Autoregressive Integrated Moving Average (ARIMA) model is often used in landslide prediction and forecasting. However, few conditions have been suggested for the application of ARIMA models in landslide displacement prediction. This paper summarizes the distribution law of the tangential angle in different time periods and analyzes the landslide displacement data by combining wavelet transform. It proposes an applicable condition for the ARIMA model in the field of landslide prediction: when the landslide deformation is in the initial deformation to initial acceleration stage, i.e., the tangential angle of landslide displacement is less than 80°, the ARIMA model has higher prediction accuracy for 24-h landslide displacement data. The prediction results are RMSE = 4.52 mm and MAPE = 2.39%, and the prediction error increases gradually with time. Meanwhile, the ARIMA model was used to predict the 24-h displacements from initial deformation to initial acceleration deformation for the landslide in Guangna Township and the landslide in Libian Gully, and the prediction results were RMSE = 1.24 mm, MAPE = 1.34% and RMSE = 5.43 mm, MAPE = 1.67%, which still maintained high accuracy and thus verified this applicable condition. At the same time, taking the landslide of Libian Gully as an example, the ARIMA model was used to test the displacement prediction effect of the landslide in the Medium-term acceleration stage and the Imminent sliding stage (the tangential angle of landslide displacement is 80° and 85°, respectively). The relative error of displacement data prediction in the Medium-term acceleration stage is within 3%, while the relative error of the prediction value in the Imminent sliding stage is more than 3%, and the error gradually increases with time. This demonstrates that the relative error of the ARIMA model in landslide prediction and forecasting is within 3%. The relative error of the prediction value in the Imminent sliding stage is above 3%, and the error increases gradually with time. Meanwhile, the prediction results are analyzed and it is concluded that the increase in prediction time and tangential angles are the main reasons for the increase in error. The applicable conditions proposed in this study can provide a reference for the application of ARIMA model in landslide prediction and forecast.
Novel engineered B lymphocytes targeting islet-specific T cells inhibit the development of type 1 diabetes in non-obese diabetic Scid mice
Authors: Chen Dawei , Kakabadse Dimitri , Fishman Sigal , Weinstein-Marom Hadas , Davies Joanne , Boldison Joanne , Thayer Terri C. , Wen Li , Gross Gideon , Wong F. Susan 
Published: September 2023

IntroductionIn this study, we report a novel therapeutic approach using B lymphocytes to attract islet-specific T cells in the non-obese diabetic (NOD) mouse model and prevent the development of autoimmune diabetes. Rather than using the antibody receptor of B cells, this approach utilizes their properties as antigen-presenting cells to T cells.MethodsPurified splenic B cells were treated with lipopolysaccharide, which increases regulatory B (Breg) cell function, then electroporated with mRNA encoding either chimeric MHC-I or MHC-II molecules covalently linked to antigenic peptides. Immunoregulatory functions of these engineered B cells (e-B cells) were tested by in vitro assays and in vivo co-transfer experiments with beta-cell-antigen-specific CD8+ or CD4+ T cells in NOD.Scid mice, respectively.ResultsThe e-B cells expressing chimeric MHC-I-peptide inhibited antigen-specific CD8+ T-cell cytotoxicity in vitro. The e-B cells expressing chimeric MHC-II-peptide induced antigen-specific CD4+ T cells to express the regulatory markers, PD-1, ICOS, CTLA-4, Lag3, and Nrp1. Furthermore, e-B cells encoding the chimeric MHC-I and MHC-II peptide constructs protected NOD.Scid mice from autoimmune diabetes induced by transfer of antigen-specific CD8+ and CD4+ T cells.DiscussionMHC–peptide chimeric e-B cells interacted with pathogenic T cells, and protected the host from autoimmune diabetes, in a mouse model. Thus, we have successfully expressed MHC–peptide constructs in B cells that selectively targeted antigen-specific cells, raising the possibility that this strategy could be used to endow different protective cell types to specifically regulate/remove pathogenic cells.
Targeting glutamine metabolic reprogramming of SLC7A5 enhances the efficacy of anti-PD-1 in triple-negative breast cancer
Authors: Huang Renhong , Wang Han , Hong Jin , Wu Jiayi , Huang Ou , He Jianrong , Chen Weiguo , Li Yafen , Chen Xiaosong , Shen Kunwei , Wang Zheng 
Published: September 2023

BackgroundTriple-negative breast cancer (TNBC) is a heterogeneous disease that is characterized by metabolic disruption. Metabolic reprogramming and tumor cell immune escape play indispensable roles in the tumorigenesis that leads to TNBC.MethodsIn this study, we constructed and validated two prognostic glutamine metabolic gene models, Clusters A and B, to better discriminate between groups of TNBC patients based on risk. Compared with the risk Cluster A patients, the Cluster B patients tended to exhibit better survival outcomes and higher immune cell infiltration. In addition, we established a scoring system, the glutamine metabolism score (GMS), to assess the pattern of glutamine metabolic modification.ResultsWe found that solute carrier family 7 member 5 (SLC7A5), an amino acid transporter, was the most important gene and plays a vital role in glutamine metabolism reprogramming in TNBC cells. Knocking down SLC7A5 significantly inhibited human and mouse TNBC cell proliferation, migration, and invasion. In addition, downregulation of SLC7A5 increased CD8+ T-cell infiltration. The combination of a SLC7A5 blockade mediated via JPH203 treatment and an anti-programmed cell death 1 (PD-1) antibody synergistically increased the immune cell infiltration rate and inhibited tumor progression.ConclusionsHence, our results highlight the molecular mechanisms underlying SLC7A5 effects and lead to a better understanding of the potential benefit of targeting glutamine metabolism in combination with immunotherapy as a new therapy for TNBC.
Early innate immune responses in European sea bass (Dicentrarchus labrax L.) following Tenacibaculum maritimum infection
Authors: Ferreira Inês A. , Peixoto Diogo , Losada Ana Paula , Quiroga María Isabel , Vale Ana do , Costas Benjamín 
Published: September 2023

IntroductionThe marine aquaculture industry has been witnessing a worldwide emergence of tenacibaculosis, a poorly understood bacterial disease caused by Tenacibaculum maritimum that affects commercially important fish. So far, knowledge on the T. maritimum virulence mechanisms is scarce and the pathogen-host interaction operating in tenacibaculosis remain to be disclosed. This study aimed at contributing to a better understanding of this disease, by evaluating the early innate immune response triggered in European sea bass (Dicentrarchus labrax) by a bath-challenge with T. maritimum.MethodsGroups of sea bass were bath-challenged with T. maritimum (challenged fish) or mock-challenged. Undisturbed fish were used as controls (time 0). Samples of blood, liver and mucosal organs (skin, gills and posterior-intestine) were collected at 0 h (control) and at 6, 24, 48 and 72 h post-challenge (n=12). Mucosal organs were used for analyzing the expression of immune-related genes by RT-qPCR, as well as blood samples for assessing haematological and innate humoral parameters and liver for oxidative stress assessment.ResultsAn increased expression of il-1β, il8, mmp9 and hamp1 was detected in all mucosal organs of infected fish when compared with control and mock-challenged fish, suggesting a pro-inflammatory response against T. maritimum transversal to all organs. The faster induction of these pro-inflammatory genes was observed in the gills. Regarding the systemic response, challenged fish presented neutrophilia, monocytosis, signs of anemia, and a decrease of bactericidal and lysozyme activities in plasma. Almost no variations were observed regarding hepatic oxidative stress.Discussion/ConclusionsThe present study suggests that T. maritimum induces a local innate immune response upon bath infection not only in the skin of European sea bass, but also in the gills and posterior-intestine, likely triggered by the T. maritimum’s capacity to adhere, colonize and damage these organs that can function as entry ways to bacteria, leading ultimately to the seen host’s systemic response.
The epithelial transcriptome and mucosal microbiota are altered for goats fed with a low-protein diet
Authors: Wu Jian , Tian Changxin , Jiao Jinzhen , Yan Qiongxian , Zhou Chuanshe , Tan Zhiliang 
Published: September 2023

IntroductionFeeding low protein (LP) diet to animals impose severe challenge to animals' immune homeostasis. However, limited knowledge about the underlying adaption mechanism of host and ruminal microbiota responding to LP diet were well understood. Herein, this study was performed to examine the changes in relative abundance of ruminal microbiota and host ruminal mucosal transcriptome profiles in response to a LP diet.MethodsA total of twenty-four female Xiangdong balck goats with similar weight (20.64 ± 2.40 kg) and age (8 ± 0.3 months) were randomly assigned into two groups, LP (5.52% crude protein containing diet) and CON (10.77% crude protein containing diet) groups. Upon completion of the trial, all goats were slaughtered after a 16-hour fasting period in LiuYang city (N 28°15′, E 113°63′) in China. HE staining, free amino acids measurement, transcriptome analysis and microbiome analysis were applied to detect the morphology alterations, free amino acids profile alterations and the shift in host ruminal mucosal transcriptome and ruminal microbiota communities.ResultsFirstly, the results showed that feeding LP diet to goats decreased the rumen papilla width (P = 0.043), surface area (P = 0.013) and total ruminal free amino acids concentration (P = 0.016). Secondly, microbiome analysis indicated that 9 microbial genera, including Eubacterium and Prevotella, were enriched in LP group while 11 microbial genera, including Butyrivibrio and Ruminococcus, were enriched in CON group. Finally, in terms of immune-related genes, the expression levels of genes involved in tight junction categories (e.g., MYH11, PPP2R2C, and MYL9) and acquired immunity (e.g., PCP4 and CXCL13) were observed to be upregulated in the LP group when compared to the CON group.ConclusionUnder the LP diet, the rumen exhibited increased relative abundance of pathogenic microbiota and VFA-degrading microbiota, leading to disruptions in immune homeostasis within the host's ruminal mucosa. These findings indicate that the ruminal microbiota interacts with host results in the disruption in animals' immune homeostasis under LP diet challenge.