bluuebunny/crossref_metadata_2025_split huggingface dataset:
Osteomodulin down-regulation is associated with osteoarthritis development
Authors: zappia Jeremie , Tong Qiao , Cruyssen Renée Van der , Cornelis Frederique , Lambert Cécile , Coelho Tiago Pinto , Grisart Juliane , Kague Erika , Lories Rik , Muller Marc , Elewaut Dirk , Hammond Chrissy , Sanchez Christelle , Henrotin Yves 
Published: January 2023

Abstract
Abnormal subchondral bone remodeling leading to sclerosis is a main feature of osteoarthritis (OA) and Osteomodulin (OMD), a proteoglycan involved in extracellular matrix mineralization, is associated to the sclerotic phenotype. However, the functions of OMD remain poorly understood, specifically in vivo. We used knock-out and overexpressing male mice for Omd and mutant zebrafish to study its roles in bone and cartilage metabolism and in the development of OA. The expression of Omd is deeply correlated to bone and cartilage microarchitectures affecting the bone volume and the onset of subchondral bone sclerosis and spontaneous cartilage lesions. Mechanistically, OMD binds to RANKL and inhibits osteoclastogenesis; thus controlling the balance of the bone remodeling. In conclusions, OMD is a key factor in subchondral bone sclerosis associated with OA. It participates in bone and cartilage homeostasis acting on the regulation of osteoclastogenesis. Targeting OMD may be a promising new and personalized approach for OA.
Increase in the Rate of Gut Carriage of Fluoroquinolone-Resistant Escherichia coli despite a Reduction in Antibiotic Prescriptions.
Authors: Tchesnokova Veronika , Larson Lydia , Basova Irina , Sledneva Yulia , Choudhury Debarati , Heng Jennifer , Solyanik Thalia , Bonilla Teresa , Pham Sophia , Schartz Ellen , Madziwa Lawrence , Holden Erika , Weissman Scott , Ralston James , Sokurenko Evgeni 
Published: January 2023

Abstract
Background: Fluoroquinolone use for urinary tract infections has been steadily declining. Gut microbiota is the main reservoir for uropathogenic Escherichia coli but whether the carriage of fluoroquinolone-resistant E. coli has been changing is unknown.
Methods. We determined the frequency of isolation and other characteristics of E. coli nonsuceptible to fluoroquinolones (at ³0.5 mg/L of ciprofloxacin) in 515 and 1605 E. coli-positive fecal samples collected in 2015 and 2021, respectively, from non-antibiotic- taking women of age 50+ receiving care in the Seattle area Kaiser Permanente Washington healthcare system.
Results. Between 2015 and 2021 the prescription of fluoroquinolones dropped nearly three-fold in the study population. During the same period, the rates of gut carriage of fluoroquinolone-resistant E. coli increased from 14.4 % to 19.9% (P=.005), driven by a significant increase of isolates from the recently emerged, pandemic multi-drug resistant clonal group ST1193 (1.7% to 4.3%; P=.007) and those with an incomplete set of or no fluoroquinolone-resistance determining mutations (2.3% to 7.5%; P<.001). While prevalence of the resistance-associated mobile genes among the isolates dropped from 64.1% to 32.6% (P<.001), co-resistance to third generation cephalosporins has increased 21.5% to 33.1%, P=.044).
Conclusion. Despite reduction in fluoroquinolone prescriptions, gut carriage of fluoroquinolone-resistant uropathogenic E. coli increased with a rise of previously sporadic lineages and co-resistance to third generation cephalosporins. Thus, to reduce the rates of antibiotic resistant urinary tract infections, greater focus should be on controlling the gut carriage of resistant bacteria.
2D resistivity model around the rupture area of the 2011 Tohoku-oki earthquake (Mw 9.0)
Authors: Ichihara Hiroshi , Kasaya Takafumi , Baba Kiyoshi , Goto Tada-nori , Yamano Makoto 
Published: January 2023

Abstract
The 2011 Tohoku-oki earthquake (Mw 9.0) was characterized by a huge fault slip on the shallowest part of the plate interface, where fault behavior had been believed to be aseismic. In this study, we modeled the two-dimensional resistivity distribution across the slip area based on ocean-bottom electromagnetic investigations to understand the physical properties around the plate interface controlling fault rupture processes. The optimal 2D resistivity model showed a conductive area around the shallowest plate interface where the huge coseismic slip was observed, whereas the deeper plate interface where the fault rupture was nucleated was relatively more resistive. The shallowest plate interface was interpreted to have a high pore seawater fraction, whereas the deeper interface was interpreted as a dry area. These findings are consistent with the hypothesis that aseismic frictional conditions changed to conditions enhancing fault rupture when the rupture propagated to the wet, clay-rich shallowest plate area. The optimal resistivity model also revealed a conductive area under the outer rise area of the Pacific Plate. This finding supports the existence of hydrated oceanic crust that supplied aqueous water to the subduction zone, including to the huge fault slip area.
Effectiveness and cost-effectiveness of a progressive, individualised walking and education program for prevention of low back pain recurrence in adults: statistical analysis plan for the WalkBack randomised controlled trial.
Authors: Pocovi Natasha , Graham Petra L , Lin Chung-Wei Christine , French Simon D , Latimer Jane , Merom Dafna , Tiedemann Anne , Maher Christopher G , Dongen Johanna M van , Clavisi Ornella , Hancock Mark J 
Published: January 2023

Abstract
Background

Exercise for the prevention of low back pain recurrences is recommended, but under-researched. The effectiveness and cost-effectiveness of a walking program for preventing low back pain recurrence remains unknown. This a priori statistical analysis plan describes the methods of analysis for the WalkBack Trial.

Methods

WalkBack is a prospectively registered, pragmatic, randomised controlled trial. The aim is to investigate the effectiveness and cost-effectiveness of a six-month progressive and individualised walking and education program (intervention) for the prevention of low back pain recurrences, compared to a no treatment control group. The primary outcome is days to first recurrence of an episode of activity-limiting low back pain. Key secondary outcomes include days to any recurrence of low back pain, a care-seeking recurrence of low back pain, disability level, health-related quality of life, costs associated with low back pain and adverse events. All participants will be followed for a minimum of 12 months. Analysis will follow the intention-to-treat principle. Cox regression is planned to assess effects for the outcomes of time to activity-limiting, minimal and care seeking recurrence. Hazard ratios and median survival times with 95% confidence intervals will be calculated. The effect of the intervention on continuous outcomes will be estimated with repeated-measure linear mixed models. An economic evaluation will be performed from the societal perspective for recurrence prevented (yes/no) and quality-adjusted life years. The proportion of adverse events between groups will be compared using Fisher’s exact test.

Discussion

The WalkBack Trial will provide evidence on the effectiveness and cost-effectiveness of a walking intervention to prevent low back pain recurrences. This statistical analysis plan provides transparency on the analysis of the trial.
Trial Registration: ACTRN12619001134112.
Plasma miRNAs and the risk of cancer: Data mining model in lung cancer
Authors: Duan Xiaoran , Huang Tao , Feng Xiaolei , Qu Xiaoping , Ge Minghui , Yan Linlin , Guo Hao , Liu Xiaohua , Ding Mingcui , Wang Pengpeng , Yang Yongli , Wang Wei , Zhao Jie 
Published: January 2023

AbstractData mining(DM) has been widely used in researching the auxiliary diagnosis of cancer. Circulating miRNAs are related to the occurrence and development of various cancer types. For this reason, they have the potential to be used as biomarkers for early tumor diagnosis. Previously, we found that SVM model combined with plasma miRNAs biomarkers could be a method for lung cancer prediction; However, it still has some limitations. So this study further enlarges the analysis to other DM techniques, and explores more accurate methods for auxiliary diagnosis of lung cancer. Univariate analysis showed the differences had statistical significance in the smoking, fever, chest tightness or pain, cough, bloody phlegm, haemoptysis, and 10 plasma miRNAs (miR-21, miR-20a, miR-210, miR-145, miR-126, miR-223, miR-197, miR-30a, miR-30d, and miR-25) between the lung cancer group and normal control group (P < 0.05); Logistic regression analysis showed that fever, chest pain or tightness, cough, miR-21, and miR-223 could be considered as indicators of the presence of cancer (P < 0.05). According to the univariate and multivariate analysis results, two sets of models were constructed using data mining models. The results showed that the sensitivity was 88.6%, the specificity reached 86.7%, the accuracy value was also the highest, and AUC was 0.877 for the GBDT (Gradient Boosting Decision Tree) 16-model, indicating that its predictive effect was the best. Conclusively, this study was to further explore the high accuracy data mining model of lung cancer prediction using plasma miRNAs.
Genomic profiles of IDH-mutant gliomas: MYCN amplified IDH-mutant astrocytoma had the worst prognosis
Authors: Lee Kwanghoon , Kim Seong-Ik , Kim Eric Eunshik , Shim Yu-Mi , Won Jae-Kyung , Park Chul-Kee , Choi Seung Hong , Yun Hongseok , Lee Hyunju , Park Sung-Hye 
Published: January 2023

Abstract
This studyprogression-freene the genetic landscape and prognostic factor of IDH-mutant gliomas. Next-generation sequencing (NGS) using a brain tumor-targeted gene panel, methylation profiles, and clinicopathological features were analyzed for O_IDH_mut (n = 74) in 70 patients and for A_IDH_mut (n = 95) in 90 patients. 97.3% of O_IDH_mut and 98.9% of A_IDH_mut displayed a classic genomic landscape. CIC and/or FUBP1 mutations were detected in 93.2% and MGMTp methylation in 95.9% of O_IDH_mut patients. In A_IDH-mut, TP53 mutations were found in 86.3% and combined ATRX (82.1%) and TERTp (6.3%) mtations in 88.4%. Although there were 3 confusing cases, NOS (not otherwise specified) category, based on genetic profiles, but they were clearly classified by combining histopathology and DKFZ methylation classifier algorithms. The patients with MYCN amplification and/or CDKN2A/2B homozygous deletion in the A_IDH_mut category had a worse prognosis than those without these gene alterations. However, there was no prognostic genetic marker in O_IDH_mut. In histopathologically or genetically ambiguous cases, methylation profiles can be used as an objective tool to avoid a diagnosis of NOS or NEC (not elsewhere classified), as well as for tumor classification. The authors have not encountered a case of true mixed oligoastrocytoma using an integrated diagnosis of genetic and methylation profiles. MYCN amplification, in addition to CDKN2A/2B homozygous deletion, should be included in the genetic criteria for WHO grade 4 A_IDH_mut.
Semi-Supervised Interlayer Intelligent Recognition Method
Authors: Jiao Shixiang , Zhao Jun , He Yufei , Wu Zhenguan , Chen Yan , Zeng Tianyi , Zhang Rui 
Published: January 2023

Abstract
The development and distribution of interlayers in sandstones can lead to increased formation heterogeneity within the reservoir, whichand further affects the movement of fluids in the formation. Therefore, it is mandatory to evaluate the interlayers accurately the fine evaluation of interlayers in sandstones is of great significance for identifying the distribution of underground the fluid systems. The logging data of interlayers are inappropriate for traditional Machine Learning training due to their low measurement proportion compared to the conventional layers. In logging data, the amount of data in interlayers is small compared to conventional reservoirs. Traditional machine learning models are mostly based on samples with balanced distribution,. By contrast, while semi-supervised learning uses requires small labeled samples for learning, and thenby Ccombineing a large number of unlabeled samples for modeling. In order to verify the effect feasibility of semi-supervised learning in the identification of interlayers, the Donghe sandstone section of H oilfield was taken as an example. First, the core analysis results were used to label the logging data; then, in order to dig out more response information that can characterize the interlayers on the logging curve, multiple features were extracted to construct cross features. Finally, an improved model based on autoencoders—probabilistic autoencoder (PAE)—is proposed to solve the problem of interlayers recognition for imbalanced samples. The PAE model can calculate a probability of belonging to a different class for unlabeled samples, and classify new samples according to the maximum probability. Experiments Results show that, compared with traditional machine learning methods and ensemble learning methods, PAE achieves higher recognition accuracy and better generalization performance by updating the algorithm, and can be used as a simple and fast method for interlayers recognition. The algorithm results prove that the semi-supervised method is of great significance for the exploration and development of complex heterogeneous oil reservoirs.The research results are of great significance for the exploration and development of complex heterogeneous oil reservoirs.
Circulating miRNA Signatures in Early-Stage Huntington’s Disease
Authors: Tao Yiran , Mercaldo Nathaniel , Duffy Alexandra , Dayananthan Ashok , Wheelock Vicki L. , Rosas Herminia Diana 
Published: January 2023

Abstract
Huntington’s Disease (HD) is a neurodegenerative disorder caused by the expansion of CAG repeats on exon 1 of the huntingtin (htt) gene. This mutation results in the expression of an aberrant protein, mutant HTT, which sets in place a cascade of events that eventually leads to neuronal death within the basal ganglia and cerebral cortex. MicroRNAs (miRNAs) are a class of small non-coding RNAs of 18 - 22 nucleotides long that play important roles in post-transcriptional regulation due to their abilities to interact with the 3'-UTR regions of mRNAs. Though generated in the nucleus, a significant portion of miRNAs are secreted into the plasma as free molecules or in vesicles for intercellular signaling. Those circulating miRNAs may provide a unique opportunity to study important pathophysiological mechanisms in HD in a non-invasive manner due to their resistance to degradation, ease of detection, and their known regulatory roles in response to inflammation and neurodevelopmental disorders. More recent studies have suggested that miRNA could be used in therapeutic applications. In this study, we sought to identify the aberrant expression of specific miRNAs extracted from the plasma of early-stage HD patients.
Clinical Trial Registration number: NCT01937923
Assessment of resources for physical activity and understanding people’s perception and practices regarding physical activity in an Indian city
Authors: Gautam Susheel , N Kruthika B , . Akashanand , Roy Aaheli , Banandur Pradeep , Banavaram Arvind Anniappan 
Published: January 2023

Abstract
Background
Promoting physical activity in urban India is imperative considering the burden of non-communicable diseases in the country. Planning for improving population level physical activity needs sound understanding of availability and quality of resources/facilities for physical activity and knowing people’s perception and practices regarding the physical activity.
Methods
A cross-sectional study was undertaken in Kolar city of Karnataka state in India. All the resources/facilities required for supporting physical activity were mapped and their quality was assessed utilizing adapted version of physical activity resource assessment questionnaire. The information regarding latitude, longitude and approximate size of the resource was obtained using a hand-held GPS tracker. 495 individuals aged ≥ 18 years, selected by two stage cluster random sampling with probability proportionate to population size technique, were interviewed to assess their perception and practices regarding physical activity using semi-structured questionnaire and global physical activity questionnaire.
Results
Kolar city has 36.3 physical activity resources per lakh population and per person availability of park and playground area was 0.4 Sq. meters. Available resources were concentrated in the center of the city. Half of the sports facilities and 14 of the 17 recreational facilities in the city were of poor to mediocre quality. 38.2% of adults in Kolar city were found to be physically active. Only 19.2% of the study participants had accessed sports/fitness facilities/playgrounds in past 3 months and only 18.8% of the study participants accessed parks in the previous 3 months. 28.6–59.1% of the participants preferred ‘walking’ as a mode of transport for work, college and shopping. Less than 5% of the participants preferred and used cycle as a mode of transport. 1/3rd of the study participants felt that Kolar city is safe of walking and 44.6% felt that the city is safe for cycling.
Conclusion
Creating enabling environment by increasing the number and quality of resources/facilities for physical activity along with their equitable distribution is required to promote and improve population level physical activity in Kolar city. Urban planning with a focus on non-motorized transport including walking would contribute to improved people’s perception and practices regarding physical activity in the city.
Study on Determination and Prediction Method of ultra-micro-volume Adhesive Droplet Volume Parametres
Authors: Zhao Dingrui , Liu Huifang , Chen Xi , Chang Yunlong , Yu Xingfu , Li An 
Published: January 2023

Abstract
Nowadays, in the actual microelectronic packaging process, the requirements for packaging quality and accuracy are becoming higher and higher. The research and prediction of the corresponding parameters of the transfer droplet according to the actual requirements before packaging can not only accurately adjust the relevant configuration parameters of the transfer droplet, but also judge the actual packaging effect preliminarily, and to improve the packaging efficiency and accuracy. Therefore, it is of practical significance to carry out the research on the distribution law and prediction method of transfer droplet parameters. In this paper, based on the needle transfer ultra-micro-volume transfer droplet method, a transfer droplet device that meets the packaging requirements is built, and the reasonable configuration of device parameters is realized through the control system. The pixel method suitable for practical application was found to determine the volume of transfer droplet, and the average deviation of the volume of transfer droplet measured by this method was about 1.85%. By changing the initial distance of the adhesive distribution experiment, it is concluded that the larger the initial distance, the smaller the volume of the transfer droplet, and the smaller the contact area of the transfer droplet. According to the data obtained from the adhesive distribution experiment, the prediction formula of the relationship between the initial distance, the volume of the transfer droplet and the contact area of the transfer droplet is derived. It is found that the average difference rate between the predicted volume and the actual volume is 0.51%, and the average difference rate between the predicted contact area and the actual contact area is 0.56%.
IRAK4 inhibition: an effective strategy for immunomodulating peri-implant osseointegration via reciprocally-shifted polarization in the monocyte-macrophage lineage cells
Authors: Zhao Juan , Li Jia , Xu Antian , Xu Yangbo , Mao Yingjie , He Fuming 
Published: January 2023

Abstract
Background: The biomaterial integration depends on its interaction with the host immune system. Monocyte-macrophage lineage cells are immediately recruited to the implant site, polarized into different phenotypes, and fused into multinucleated cells, thus playing roles in tissue regeneration. IL-1R-associated kinase 4 (IRAK4) inhibition was reported to antagonize inflammatory osteolysis and reciprocally regulate osteoclasts and foreign body giant cells (FBGCs), which may be a potential target in implant osseointegration.
Methods:In in-vitro experiments, we established simulated physiological and inflammatory circumstances in which bone-marrow-derived macrophages were cultured on sand-blasted and acid-etched (SLA) titanium surfaces to evaluate the induced macrophage polarization, multinucleated cells formation, and biological behaviors in the presence or absence of IRAK4i. Then, bone marrow stromal stem cells (BMSCs) were cultured in the conditioned media collected from the aforementioned induced osteoclasts or FBGCs cultures to clarify the indirect coupling effect of multinucleated cells on BMSCs. We further established a rat implantation model, which integrates IRAK4i treatment with implant placement, to verify the positive effect of IRAK4 inhibition on the macrophage polarization, osteoclast differentiation, and ultimately the early peri-implant osseointegration in vivo.
Results: Under inflammatory conditions, by transforming the monocyte-macrophage lineage cells from M1 to M2, IRAK4i treatment could down-regulate the formation and activity of osteoclast and relieve the inhibition of FBGC generation, thus promoting osteogenic differentiation in BMSCs and improve the osseointegration.
Conclusions: This study may improve our understanding of the function of multinucleated cells and offer IRAK4i as a therapeutic strategy to improve early implant osseointegration and help to eliminate the initial implant failure.
A mobile car monitoring system as a supplementary tool for air quality monitoring in urban and rural environments. The case study from Poland
Authors: Maslouski Mikita , Jarosz-Krzemińska Elżbieta , Jagoda Paweł , Adamiec Ewa 
Published: January 2023

Abstract
Living in healthy environment should be regarded as a primary human right and not a privilege rendered to chosen ones. For that reason, a national air monitoring grid should be as extensive as possible. Unfortunately, small towns and villages, which are also exposed to air pollution episodes are not commonly covered by monitoring grid. Fixed air monitoring systems have their limitations, which can be overcome by e.g., properly validated, reliable but cheaper mobile monitoring systems. The aim of this study was to assess the use of a car mobile PM10 monitoring system to study ambient air quality in rural communities surrounding Kraków, not covered by fixed monitoring grid. A monitoring dataset was collected during 6 winter car campaigns conducted between December 2021 and March 2022. Except for providing multiple monitoring data, the main goal pertain to methodological aspect of the mobile system, including its validation, indicating its advantages, limitations as well as providing recommendations for the future mobile studies. Our car mobile monitoring system was useful in identifying a local hot-spots with good spatial and temporal resolution, thus giving the local government decision-making tool for taking appropriate action in places not included in national monitoring grid.