bluuebunny/crossref_metadata_2025_split huggingface dataset:
Timing of dense granule biogenesis in asexual malaria parasites
Authors: Vallintine Tansy , van Ooij Christiaan 
Published: June 2023

ABSTRACTMalaria is an important infectious disease that continues to claim hundreds of thousands of lives annually. The disease is caused by infection of host erythrocytes by apicomplexan parasites of the genusPlasmodium. The parasite contains three different apical organelles – micronemes, rhoptries and dense granules – whose contents are secreted to mediate binding to and invasion of the host cell and the extensive remodelling of the host cell that occurs following invasion. Whereas the roles of micronemes and rhoptries in binding and invasion of the host erythrocyte have been studied in detail, the role of dense granules (DGs) inPlasmodiumparasites are poorly understood. They have been proposed to control host cell remodelling through regulated protein secretion after invasion, but many basic aspects of the biology of DGs remain unknown. Here we describe DG biogenesis timing for the first time, using RESA localisation as a proxy for DG formation timing. We show that DG formation commences approximately 37 minutes prior to schizont egress, as measured by the recruitment of the DG marker RESA. Furthermore, using a bioinformatics approach, we aimed to predict additional cargo of the DGs and identified the J-dot protein HSP40 as a DG protein, further supporting the very early role of these organelles in the interaction of the parasite with the host cell.
Rational engineering of an elevator-type metal transporter ZIP8 reveals a conditional selectivity filter critically involved in determining substrate specificity
Authors: Jiang Yuhan , Li Zhen , Sui Dexin , Sharma Gaurav , Wang Tianqi , MacRenaris Keith , Takahashi Hideki , Merz Kenneth , Hu Jian 
Published: June 2023

AbstractEngineering of transporters to alter substrate specificity as desired holds great potential for applications, including metabolic engineering. However, the lack of knowledge on molecular mechanisms of substrate specificity hinders designing effective strategies for transporter engineering. Here, we applied an integrated approach to rationally alter the substrate preference of ZIP8, a Zrt-/Irt-like protein (ZIP) metal transporter with multiple natural substrates, and uncovered the determinants of substrate specificity. By systematically replacing the differentially conserved residues with the counterparts in the zinc transporter ZIP4, we created a zinc-preferring quadruple variant (Q180H/E343H/C310A/N357H), which exhibited largely reduced transport activities towards Cd2+, Fe2+, and Mn2+whereas increased activity toward Zn2+. Combined mutagenesis, modeling, covariance analysis, and computational studies revealed a conditional selectivity filter which functions only when the transporter adopts the outward-facing conformation. The demonstrated approach for transporter engineering and the gained knowledge about substrate specificity will facilitate engineering and mechanistic studies of other transporters.
Deep learning image analysis for filamentous fungi taxonomic classification: Dealing with small data sets with class imbalance and hierarchical grouping
Authors: Stiller Stefan , Dueñas Juan F. , Hempel Stefan , Rillig Matthias C. , Ryo Masahiro 
Published: June 2023

AbstractDeep learning applications in taxonomic classification for animals and plants from images have become popular, while those for microorganisms are still lagging behind. Our study investigated the potential of deep learning for the taxonomic classification of hundreds of filamentous fungi from colony images, which is typically a task that requires specialized knowledge. We isolated soil fungi, annotated their taxonomy using standard molecular barcode techniques, and took images of the fungal colonies grown in petri dishes (n = 606). We applied a convolutional neural network with multiple training approaches and model architectures to deal with some common issues in ecological datasets: small amounts of data, class imbalance, and hierarchically structured grouping. Model performance was overall low due mainly to the relatively small data set, class imbalance, and the high morphological plasticity exhibited by fungal colonies. However, our approach indicates that morphological features such as color, patchiness, and colony extension rate, could be used for the recognition of fungal colonies at higher taxonomic ranks (i.e., phylum, class, and order). Model explanation implies that image recognition characters appear at different positions within the colony (e.g., outer or inner hyphae), depending on the taxonomic resolution. Our study suggests the potential of deep learning applications for better understanding the taxonomy and ecology of filamentous fungi amenable to axenic culturing. Meanwhile, our study also highlights some technical challenges in deep learning image analysis in ecology, highlighting that the domain of applicability of these methods needs to be carefully considered.
Legitimacy-building role of incubators: a multiple case study of activities and impacts of business incubators in a developing Chinese city
Authors: Cheng Ying , Liu Yanyan , Cross Adam R. 
Published: June 2023

Purpose
Business incubators are advantageous to new venture legitimacy because they provide rich access to entrepreneurial resources, and their incubation networks can offer endorsement to incubatees. However, empirical evidence on this topic is limited, and the existing literature relies predominantly on the Western context. Given that not all developing country incubators have resourceful and reputable external entrepreneurial networks as in the industrialized countries, and that new ventures need to build legitimacy along cognitive and socio-political dimensions that require different actions to influence different stakeholders, this study investigates empirically how business incubators facilitate their incubatees to build legitimacy in a context where resource and reputation conditions are weak. The purpose of this paper is to clarify how business incubators perform legitimacy-building roles effectively.

Design/methodology/approach
A multiple case study of business incubators in Chongqing, a second-tier Chinese city, is presented. Using grounded theory, this paper draws its findings from a synthesis of interviews and secondary data of seven incubators and their ten incubatees.

Findings
The legitimacy-building role of business incubators is performed well in this research context. Evidence is presented that incubators play different roles in building different dimensions of incubatees’ legitimacy. Government-associated incubators play a salient role in building incubatees’ socio-political legitimacy whilst non-government related incubators shape their incubatees’ cognitive legitimacy.

Originality/value
This study contributes to the business incubators literature by revealing how incubators perform the legitimacy-building role when their resource endorsement is weak. The results suggest that incubators need to strengthen their ties with external stakeholders and that new ventures need to take key stakeholders into consideration when they select incubators to enter.
Identifying research fronts in NLP applications in library and information science using meta-analysis approaches
Authors: Majhi Debasis , Mukherjee Bhaskar 
Published: June 2023

Purpose
The purpose of this study is to identify the research fronts by analysing highly cited core papers adjusted with the age of a paper in library and information science (LIS) where natural language processing (NLP) is being applied significantly.

Design/methodology/approach
By excavating international databases, 3,087 core papers that received at least 5% of the total citations have been identified. By calculating the average mean years of these core papers, and total citations received, a CPT (citation/publication/time) value was calculated in all 20 fronts to understand how a front is relatively receiving greater attention among peers within a course of time. One theme article has been finally identified from each of these 20 fronts.

Findings
Bidirectional encoder representations from transformers with CPT value 1.608 followed by sentiment analysis with CPT 1.292 received highest attention in NLP research. Columbia University New York, in terms of University, Journal of the American Medical Informatics Association, in terms of journals, USA followed by People Republic of China, in terms of country and Xu, H., University of Texas, in terms of author are the top in these fronts. It is identified that the NLP applications boost the performance of digital libraries and automated library systems in the digital environment.

Practical implications
Any research fronts that are identified in the findings of this paper may be used as a base for researchers who intended to perform extensive research on NLP.

Originality/value
To the best of the authors’ knowledge, the methodology adopted in this paper is the first of its kind where meta-analysis approach has been used for understanding the research fronts in sub field like NLP for a broad domain like LIS.
Managers’ process thinking skills, dynamic capabilities and performance in export ventures
Authors: Vardarsuyu Merve , Spyropoulou Stavroula , Menguc Bulent , Katsikeas Constantine S. 
Published: June 2023

PurposeThe purpose of this study is to unfold the role of managerial characteristics in developing the dynamic capabilities necessary to serve foreign customers and compete in export market ventures.Design/methodology/approachThe authors test their proposed model using path analysis with data collected from export managers working in 204 small- and medium-sized Turkish exporters operating in various sectors.FindingsThe findings suggest that the positive effect of export managers’ process thinking skills on dynamic capabilities increases when the export managers’ learning and avoid orientations are low and prove orientation is high and export venture experience (duration and scope) increases. In addition, it has been found that export managers’ process thinking skills have an indirect effect on export performance through export venture dynamic capabilities.Originality/valueThis study makes three contributions. First, the authors conceptualize and operationalize dynamic capabilities in the context of exporting. The authors empirically validate export venture dynamic capabilities as a higher-level construct composed of sensing, seizing and reconfiguring elements pertinent to the firm’s export market operations. Second, based on the micro-foundations approach of competitive advantage, the authors study managers’ process thinking skills in exporting firms and how these abilities support dynamic capability development in export ventures. Finally, the authors investigate how the impact of export managers’ process thinking skills on export venture dynamic capabilities is influenced by their goal orientations and certain objective exporter characteristics pertaining to different aspects of export venture experience.
Financial integration and earnings management: evidence from emerging markets
Authors: Shah Syed Zulfiqar Ali , Wan Fangyi 
Published: June 2023

PurposeThis study examines whether country-level financial integration affects firms' accounting choices and the quality of financial information.Design/methodology/approachThis study employs Propensity Score Matching (PSM), and panel regressions of a large sample of data from 20 emerging markets over the period 1987–2018.FindingsThis study finds evidence that increased level of financial integration is significantly positively associated with firms' accruals earnings management (AEM) and real earnings management (REM).Research limitations/implicationsFindings in the study have implications for standard-setting bodies that aim to enhance the usefulness of financial reporting quality. The study also has implications for various initiatives by governments in emerging markets aimed at raising investor confidence and fostering stock market development through greater financial integration.Practical implicationsFindings in the study have implications for standard-setting bodies that aim to enhance the usefulness and quality of financial reporting. The findings can be of interest to analysts, auditors and other monitoring institutions who play a crucial role in detecting earnings management and reducing information asymmetry. Finally, the study has implications for various initiatives by governments in emerging markets aimed at raising investor confidence and fostering stock market development through greater financial integration.Originality/valueFindings in the study reveal how country-level financial integration affects accruals and real earnings management in a sample of firms from 20 emerging markets. Further, the study adds to the growing body of literature on emerging markets where capital markets mechanisms, regulatory environment and firm's corporate governance are distinct to developed markets.
Industry 4.0 benefits, challenges and critical success factors: a comparative analysis through the lens of resource dependence theory across continents and economies
Authors: Antony Jiju , Sony Michael , Garza-Reyes Jose Arturo , McDermott Olivia , Tortorella Guilherme , Jayaraman Raja , Sucharitha Rahul Srinivas , Salentijin Wilem , Maalouf Maher 
Published: June 2023

PurposeEntering a new era of digital transformation, Industry 4.0 (I 4.0) promises to revolutionize the way business has been done, providing unprecedented opportunities and challenges. This study aims to investigate empirically and comparatively analyse the benefits, challenges and critical success factors (CSFs) of Industry 4.0 across four continents and developing and developed economies.Design/methodology/approachThis study used an online survey to explore the benefits, challenges and CSFs of developed and developing economies. In order to ensure the validity of the survey, a pilot test was conducted with 10 respondents. A total of 149 participants with senior managerial, vice-presidential and directorial positions from developed and developing economies spanning four continents were invited to take part in the survey.FindingsThe study ranks benefits, challenges and CSFs across economies and continents. Further, the benefit of Industry 4.0 helping to achieve organizational efficiency and agility differed across the developing and developed economies. Furthermore, the benefit improves customer satisfaction significantly differed across continents; in terms of challenges, Employee resistance to change had a higher proportion in developing economies. The future viability of I 4.0 also differed across the continents. Regarding CSFs, there was no difference across the developing and developed economies. Finally, change management and project management vary across the continents.Research limitations/implicationsThis study contributes to a balanced understanding of I 4.0 by providing empirical evidence for comparative analysis. Moreover, it extends the concept of resource dependence theory to explain how organizations in developing economies and developed economies deploy resources to manage external condition uncertainties to implement I 4.0. Furthermore, this study provides a structural framework to understand the specific benefits, challenges and CSFs of implementing I 4.0, which can be utilized by policymakers to promote I 4.0 in their economies or continents.Originality/valueTo the best of the authors’ knowledge, no studies have empirically demonstrated the comparative analysis of benefits, challenges and CSFs across economies and continents and distinguish an original contribution of this work.
Social marketing framework for anti-littering behavior: an integrated serial mediation model
Authors: Kaur Ranjit , Singh Jagwinder 
Published: June 2023

Purpose
Littering has been identified as a significant social issue in India that has negative impact on the environment as well as public health. Social marketing is a powerful tool for changing and sustaining pro-environmental behaviors, but so far no research has been found on the application of social marketing on changing littering behavior in India. This paper aims to integrate the two widely used social marketing models, health belief model (HBM) and theory of planned behavior (TPB), with an extra factor, environmental factors, in a holistic model to understand and predict anti-littering behavior in India.

Design/methodology/approach
In total, 750 individuals were interviewed at 34 locations across the states of New Delhi and Punjab (India) in a field survey. For data analysis, confirmatory factor analysis and structural equation model techniques in AMOS 21.0 have been used.

Findings
The results of the study suggest that the perceived benefits and perceived barriers have a significant direct effect on the attitude toward anti-littering behavior. No direct impact of perceived threat has been found on attitude toward anti-littering behavior. The results also confirm the role of attitude, subjective norms and self-efficacy in influencing the anti-littering intention with attitude and subjective norms having a greater impact on intention as compared to self-efficacy. Furthermore, the results also highlight that the environmental factors and intention carry more weights than perceived behavioral control in influencing anti-littering behavior. Recognizing the importance of sequential mediators in the present study, the results of the serial mediation analysis revealed that attitude and intention sequentially mediate the path linking perceived benefits, perceived barriers and anti-littering behavior.

Originality/value
To the best of the authors’ knowledge, the current study in terms of theory building appears to be the first to integrate social marketing models HBM and TPB to explain and understand not only anti-littering behavior but also for setting a platform of behavior change in a larger context.
Understanding corporate tax responsibility: a systematic literature review
Authors: Scarpa Francesco , Signori Silvana 
Published: June 2023

Purpose
This study aims to contribute to the debate about the place of corporate taxation in corporate social responsibility (CSR) by reviewing the present state of research, offering a comprehensive understanding of the content and dimensions of corporate tax responsibility (CTR) and discussing further developments in research and action.

Design/methodology/approach
The study builds on a systematic literature review of 117 theoretical and empirical papers on tax within the broad field of CSR published in peer-reviewed academic journals and books.

Findings
The analysis unfolds and discusses the construct of CTR and proposes a unified conceptualisation that elucidates for what firms are (or should be) held accountable on tax matters and the different dimensions (i.e. instrumental, political, integrative and ethical) which justify greater tax responsibility and enable its achievement.

Practical implications
The results can provide companies with practical guidance to enhance their tax responsibility and can give stakeholders and policymakers suggestions for new mobilisation strategies to achieve more responsible tax behaviour.

Social implications
Corporate tax payments are a fundamental dimension of CSR, as they fund public goods and services and reduce the unequal distribution of wealth. Providing a more structured understanding of CTR, this paper can contribute towards attaining more responsible tax outcomes which can better serve and benefit the whole society.

Originality/value
This study offers a structured overview of the present state of tax research in CSR, while providing a comprehensive understanding and conceptualisation of the construct of CTR, thus enabling scholars to situate their work and develop further relevant research in this field.
Transgenerational competition effects persist across multiple generations and are altered by offspring competitive environments in a clonal plant
Authors: Jin Yu , Chen Jin‐Song , Lei Ning‐Fei , Luo Fang‐Li , Huang Lin , Xue Wei , Zhang Qian , Yu Fei‐Hai 
Published: June 2023

AbstractEnvironments experienced by parental plants may potentially influence the performance of their offspring. These effects may also vary depending on the current environment experienced by the offspring. However, whether these transgenerational effects, especially those induced by biotic factors such as competition, can persist for multiple generations has not been tested. Here, we examined intraspecific competition‐induced transgenerational effects across multiple generations using a floating clonal plant Spirodela polyrhiza, by growing three successive generations each under either low or high density. The second‐generation offspring performed better when the first‐generation plants were grown under low than under high density, independently of the density experienced by the second generation. The third‐generation offspring performed better under low than under high density, and the difference was more pronounced when the second‐generation plants were grown under low density. Moreover, the density of the first and second generation interacted to influence the morphology of the third‐generation offspring. These results indicate that competition‐induced transgenerational effects in S. polyrhiza can vary depending on the competition environment of its offspring and that these effects can persist across multiple generations.
Postoperative bronchopleural fistula after induction therapy with bevacizumab
Authors: Wada Hironobu , Suzuki Hidemi , Tanaka Kazuhisa , Sakairi Yuichi , Yoshino Ichiro 
Published: June 2023

AbstractBronchopleural fistulas are rare complications of bevacizumab treatment. Herein, we report a case of bronchopleural fistula after bevacizumab therapy. The patient was a 65‐year‐old man with lung cancer who underwent a right lower lobectomy with systemic lymph node dissection after induction chemotherapy with bevacizumab. Pathological examination revealed no residual tumor cells in the resected specimen. The patient presented with severe dyspnea on postoperative day 26. Bronchoscopy revealed a bronchopleural fistula in the membranous portion of the right intermediate bronchus; the bronchial stump remained intact. The bronchopleural fistula was repaired with muscle flaps, and bronchoscopy 9 months after surgery showed satisfactory healing of the fistula. The patient has been alive for 5 years without evidence of recurrence. Careful attention must be paid to postoperative management when bevacizumab is used for induction therapy.