bluuebunny/crossref_metadata_2025_split huggingface dataset:
Abl kinases can function as suppressors of tumor progression and metastasis
Authors: Marchal Melissa A. , Moose Devon L. , Varzavand Afshin , Jordan Nicole E. , Taylor Destiney , Tanas Munir R. , Brown James A. , Henry Michael D. , Stipp Christopher S. 
Published: September 2023

IntroductionAbl family kinases function as proto-oncogenes in various leukemias, and pro-tumor functions have been discovered for Abl kinases in many solid tumors as well. However, a growing body of evidence indicates that Abl kinases can function to suppress tumor cell proliferation and motility and tumor growth in vivo in some settings.MethodsTo investigate the role of Abl kinases in tumor progression, we used RNAi to generate Abl-deficient cells in a model of androgen receptor-indifferent, metastatic prostate cancer. The effect of Abl kinase depletion on tumor progression and metastasis was studied in an in vivo orthotopic model, and tumor cell motility, 3D growth, and signaling was studied in vitro.ResultsReduced Abl family kinase expression resulted in a highly aggressive, metastatic phenotype in vivo that was associated with AKT pathway activation, increased growth on 3D collagen matrix, and enhanced cell motility in vitro. Inhibiting AKT pathway signaling abolished the increased 3D growth of Abl-deficient cells, while treatment with the Abl kinase inhibitor, imatinib, promoted 3D growth of multiple additional tumor cell types. Moreover, Abl kinase inhibition also promoted soft-agar colony formation by pre-malignant fibroblasts.ConclusionsCollectively, our data reveal that Abl family kinases can function to suppress malignant cell phenotypes in vitro, and tumor progression and metastasis in vivo.
Almonertinib plus chemotherapy versus almonertinib alone in second-line treatment of advanced non-small cell lung cancer with mutated epidermal growth factor receptor: a retrospective study
Authors: Fang Xiaoxu , Xiang Yan , Lu Kaihua 
Published: September 2023

ObjectiveThis study mainly observes the efficacy and safety of almonertinib plus chemotherapy compared with almonertinib alone in the second-line treatment of advanced non-small cell lung cancer (NSCLC) with mutated epidermal growth factor receptor (EGFR).MethodsIn this study, clinical data of 68 patients with advanced NSCLC who were treated in Jiangsu Provincial People’s Hospital and Nanjing Chest Hospital between April 2020 and December 2022 were collected. Among them, the study group (n=30) received second-line almonertinib combined with platinum-based chemotherapy, while the control group (n=38) received almonertinib alone. The near-term and long-term effects and adverse events of the two groups were compared respectively.ResultsThe median follow-up time until 31 December 2022 was 16.3 months (95% CI: 11.32-21.34). Results of chi-square analysis showed no statistically significant difference in objective response rate (ORR) and disease control rate (DCR) between the study group and the control group (56.73% vs. 55.3%, P&gt;0.05; 100% vs. 86.8%, P&gt;0.05). Log-rank test comparing the two groups revealed that the median progression-free survival (mPFS) of the study group was significantly longer than that of the control group by 3.1 months (12.7 vs. 9.6 months, P=0.01). Multivariate COX proportional risk model showed a statistically significant effect of treatment method and PS score on PFS (HR=0.43, P=0.023; HR=3.82, P=0.001). In terms of safety, most of the adverse events (AEs) were mild, with no grade 4-5 in the two groups, and the overall tolerance of patients was good.ConclusionFor advanced NSCLC patients with EGFR mutations, second-line treatment with almonertinib plus chemotherapy significantly improved PFS compared with almonertinib alone without a significant increase in adverse events, providing efficacy and safety.
Evaluation of single-fraction high dose FLASH radiotherapy in a cohort of canine oral cancer patients
Authors: Børresen Betina , Arendt Maja L. , Konradsson Elise , Bastholm Jensen Kristine , Bäck Sven ÅJ. , Munck af Rosenschöld Per , Ceberg Crister , Petersson Kristoffer 
Published: September 2023

BackgroundFLASH radiotherapy (RT) is a novel method for delivering ionizing radiation, which has been shown in preclinical studies to have a normal tissue sparing effect and to maintain anticancer efficacy as compared to conventional RT. Treatment of head and neck tumors with conventional RT is commonly associated with severe toxicity, hence the normal tissue sparing effect of FLASH RT potentially makes it especially advantageous for treating oral tumors. In this work, the objective was to study the adverse effects of dogs with spontaneous oral tumors treated with FLASH RT.MethodsPrivately-owned dogs with macroscopic malignant tumors of the oral cavity were treated with a single fraction of ≥30Gy electron FLASH RT and subsequently followed for 12 months. A modified conventional linear accelerator was used to deliver the FLASH RT.ResultsEleven dogs were enrolled in this prospective study. High grade adverse effects were common, especially if bone was included in the treatment field. Four out of six dogs, who had bone in their treatment field and lived at least 5 months after RT, developed osteoradionecrosis at 3-12 months post treatment. The treatment was overall effective with 8/11 complete clinical responses and 3/11 partial responses.ConclusionThis study shows that single-fraction high dose FLASH RT was generally effective in this mixed group of malignant oral tumors, but the risk of osteoradionecrosis is a serious clinical concern. It is possible that the risk of osteonecrosis can be mitigated through fractionation and improved dose conformity, which needs to be addressed before moving forward with clinical trials in human cancer patients.
Association of dietary fatty acid intake with hypertension in children and adolescents: evidence from the NHANES 2005–2018
Authors: Li Xiumin , Qi Fengqin , Zhao Zhihong , Ma Jinbang 
Published: September 2023

AimThis study aims to evaluate the association between dietary fatty acid intake and hypertension in children and adolescents.MethodsThis cross-sectional study used data of children and adolescents aged 8–17 years from the National Health and Nutrition Examination Survey (NHANES) 2005–2018. Dietary intake of total fat and fatty acid was evaluated via two 24-h dietary recall interviews. Multivariate logistic regression models were used to assess the association between fatty acid intake and hypertension, with odds ratios (ORs) and 95% confidence intervals (CIs) calculated. A subgroup analysis was conducted according to gender, age, and body mass index Z-score.ResultsThis study included 13,330 subjects, of which 11,614 were non-hypertensive and 1,716 were hypertensive. Higher intake of total polyunsaturated fatty acids (PUFAs) was associated with significantly lower odds of hypertension (OR = 0.85, 95% CI: 0.74–0.97, P = 0.018). No significant associations were found between the density of total saturated fatty acid, monounsaturated fatty acids, and PUFAs and the odds of hypertension (all P &gt; 0.05). Increased intake of omega-3 (OR = 0.82, 95% CI: 0.72–0.93, P = 0.002) and omega-6 (OR = 0.86, 95% CI: 0.75–0.98, P = 0.025) PUFAs, octadecatrienoic acid (OR = 0.82, 95% CI: 0.72–0.93, P = 0.003), and octadecadienoic acid (OR = 0.86, 95% CI: 0.75–0.98, P = 0.025) was associated with significantly lower odds of hypertension, and individuals with higher omega-6/omega-3 ratio had significantly higher odds of hypertension (OR = 1.09, 95% CI: 1.02–1.17, P = 0.025). The density of omega-3 PUFAs (OR = 0.86, 95% CI: 0.78–0.95, P = 0.004) and octadecatrienoic acid (OR = 0.87, 95% CI: 0.78–0.96, P = 0.006) was inversely associated with the odds of hypertension, and the omega-6/omega-3 ratio was positively associated with the odds of hypertension (OR = 1.09, 95% CI: 1.02–1.17, P = 0.012).ConclusionTotal PUFA intake was negatively associated with the odds of hypertension in children and adolescents. Higher intake of omega-3 and omega-6 PUFAs, octadecatrienoic acid, and octadecadienoic acid, as well as density of omega-3 PUFAs and octadecatrienoic acid, was associated with lower odds of hypertension.
A hypothermia mimetic molecule (zr17-2) reduces ganglion cell death, gliosis, and electroretinogram distortion in male rats subjected to perinatal asphyxia
Authors: Rey-Funes Manuel , Fernández Juan Carlos , Peláez Rafael , Soliño Manuel , Contartese Daniela S. , Ciranna Nicolás S. , Nakamura Ronan , Sarotto Aníbal , Dorfman Verónica B. , Zapico José M. , Ramos Ana , de Pascual-Teresa Beatriz , López-Costa Juan José , Larrayoz Ignacio M. , Martínez Alfredo , Loidl César Fabián 
Published: September 2023

Introduction: Perinatal asphyxia (PA) represents a major problem in perinatology and may cause visual losses, including blindness. We, and others, have shown that hypothermia prevents retinal symptoms associated to PA. In the present work, we evaluate whether a hypothermia mimetic small molecule, zr17-2, has similar effects in the context of PA.Methods: Four experimental groups were studied in male rats: Naturally born rats as controls (CTL), naturally born rats injected s.c. with 50 µL of 330 nmols/L zr17-2 (ZR), animals that were exposed to PA for 20 min at 37°C (PA), and rats that were exposed to PA and injected with zr17-2 (PA-ZR). Forty-five days after treatment, animals were subjected to electroretinography. In addition, morphological techniques (TUNEL, H&amp;E, multiple immunofluorescence) were applied to the retinas.Results: A reduction in the amplitude of the a- and b-wave and oscillatory potentials (OP) of the electroretinogram (ERG) was detected in PA animals. Treatment with zr17-2 resulted in a significant amelioration of these parameters (p &lt; 0.01). In PA animals, a large number of apoptotic cells was found in the GCL. This number was significantly reduced by treatment with the small molecule (p &lt; 0.0001). In a similar way, the thickness of the inner retina and the intensity of GFAP immunoreactivity (gliosis) increased in PA retinas (p &lt; 0.0001). These parameters were corrected by the administration of zr17-2 (p &lt; 0.0001). Furthermore, injection of the small molecule in the absence of PA did not modify the ERG nor the morphological parameters studied, suggesting a lack of toxicity.Discussion: In conclusion, our results indicate that a single s.c. injection of zr17-2 in asphyctic neonates may provide a novel and efficacious method to prevent the visual sequelae of PA.
Sequential mediation of early temperament and eating behaviors in the pathways from feeding practices to childhood overweight and obesity
Authors: Zhang Xiaoning , Zhou Qiong , Vivor Nathaniel Kossi , Liu Wei , Cao Junli , Wang Sheng 
Published: September 2023

IntroductionChildhood eating behaviors and temperament may have important implication for constructing the pathways from maternal feeding practices to childhood overweight and obesity (OW/OB). Examining multiple feeding styles simultaneously to childhood OW/OB is critical through the mediators of early childhood temperament and eating behaviors.MethodsThis cross-sectional study recruited mothers mainly responsible for child care from two hospitals and two healthcare centers in eastern China. Sociodemographic characteristics, and data from the Infant Feeding Style Questionnaire (IFSQ), the short form of Children Behavior Questionnaire [Revised (IBQ-RSF)], and the Child Eating Behavior Questionnaire for toddler (CEBQ-T) were collected. Weight and recumbent length were measured to calculate the age- and sex-specific body mass index (BMI) z-scores (BMIz). The structural equation modeling (SEM) approach was used to examine direct and indirect pathways from five maternal feeding styles to childhood OW/OB through temperament and eating behaviors.ResultsA total of 486 children were recruited, 73 (15.02%) children were OW/OB; the age of the children was 14.55 (SD = 5.14) months, and the age of the mothers was 29.90 (SD = 3.63) years. The responsive feeding exerted significant direct (β = −0.098), indirect (β = −0.136) and total (β = −0.234) effects on childhood OW/OB. Restrictive feeding had significant direct (β = 0.222), indirect (β = 0.102) and total (β = 0.324) effects on childhood OW/OB. Indulgent feeding had significant direct (β = 0.220), indirect (β = 0.063), and total (β = 0.283) effects on childhood OW/OB. Pressuring feeding had significant direct (β = −0.116), indirect (β = −0.096) and total (β = −0.212) effects on childhood OW/OB.DiscussionThere was a direct effect of feeding practices on childhood OW/OB; feeding practices indirectly predicted childhood OW/OB through temperament and eating behaviors in children aged 6–23 months. This study could help governments agencies, policymakers, and healthcare workers to establish optimal intervention programs targeting feeding practices through childhood eating behaviors and temperament to prevent childhood OW/OB.
The safety and efficacy of laparoscopic retrograde appendicectomy, base-to-tip approach
Authors: Ko Ara , Lindsay Perry , Choi Julian 
Published: September 2023

BackgroundLaparoscopic appendicectomy is one of the most frequently performed surgical procedures worldwide. There is limited evidence evaluating the role and safety of laparoscopic retrograde appendicectomy (LRA), base to tip approach, compared to standard laparoscopic antegrade appendicectomy (LAA), tip to base approach. This study aims to assess the safety of LRA compared to LAA in terms of intra-abdominal collection (IAC) rate and using Sunshine Appendicitis Grading System (SAGS).MethodsRecords of two-hundred and seventy-three patients undergoing laparoscopic appendicectomy by LAA and LRA approaches were analysed. The severity of appendicitis was rated using a standardised Sunshine Appendicitis Grading System (SAGS) score intra-operatively. The primary outcome measure was the occurrence of an intra-abdominal collection, and secondary measures were procedure time, post-operative length of stay and other complications.ResultsOf the two-hundred and seventy-three patients, there were two patients who developed an intra-abdominal collection. Both patients were in the LAA group with SAGS IV scores. Between SAGS IV patients, Chi-squared p value of 0.6691. Therefore, there was no statically significant difference in the intra-abdominal collection (IAC) rate between LAA and LRA groups from this study.ConclusionsThe current study has shown that laparoscopic retrograde appendicectomy (LRA) does not increase risk of intra-abdominal collection compared to laparoscopic antegrade appendicectomy (LAA) within the limit of this study.
Evaluation of the Antifungal Effect of Rhus verniciflua Stokes Extract for Oral Application Potential
Authors: Kim Yu-Rin , Kim Gyoo-Cheon , Nam Seoul-Hee 
Published: September 2023

Background and Objectives: This study confirms the possibility of using Rhus verniciflua Stokes (RVS) extract as a natural treatment for oral candidiasis. Materials and Methods: RVS was extracted with 70% ethanol to examine the antioxidant activity through polyphenol, flavonoid content, and DPPH (1,1-diphenyl-2-picrylhydrazyl). To evaluate the antifungal effect against Candida albicans (C. albicans; KCTC 7965/ATCC 10231) and evaluate the stability of RVS, a water-soluble tetrazolium salt (WST-1) assay was performed in human keratinocytes (HaCaT). Results: The findings revealed that RVS extract has fairly high antioxidant activity. The clear zones of the RVS extract against C. albicans increased in diameter due to the inhibition of fungal growth at higher concentrations. Treatment with the 1.25 mg/mL RVS extract had a more than 99% antifungal effect against C. albicans, and the 20 mg/mL RVS extract had a 100% antifungal effect. The WST-1 assay showed that the RVS extract induced low cell viability in the HaCaT cells, which inhibited their proliferation, and the RVS extract is also toxic to normal cells. Conclusions: Although the RVS extract with high antioxidant activity showed clear antifungal activity against C. albicans, it exhibited a low survival rate. Therefore, the development of a safe natural antibiotic is necessary.
Growth, Physiology and Nutritional Quality of C4 Halophyte Portulaca oleracea L. Grown Aeroponically in Different Percentages of Artificial Seawater under Different Light-Emitting Diode Spectral Qualities
Authors: He Jie , Leng Su Yee , Qin Lin 
Published: September 2023

Edible halophyte Portulaca oleracea L., known as purslane, was grown in two percentages of artificial seawater (ASW) under two combined red (R) and blue (B) LED spectra. High salinity (40% ASW) negatively affected shoot productivity and leaf growth of purslane compared to those grown in 10% ASW. Photosynthetic pigment and total reduced nitrogen concentrations were significantly higher in purslane grown in 10% ASW than in 40% ASW. However, LED spectral quality did not markedly influence these parameters. Grown in 10% ASW under R/B 2.2, purslane had the highest maximum nitrate reductase activity, while those in 40% ASW under R/B 2.2 had the highest activation state. Under both light qualities, purslane had a sevenfold increase in proline concentration in 40% ASW than in 10% ASW. Total phenolic compounds’ concentration was the highest in 10% ASW under R/B 0.9, while there were no significant differences in the accumulation of total soluble sugars and ascorbic acids among all plants. Antioxidant enzymes activities were lower in 40% ASW under R/B 2.2 compared to the other conditions. In conclusion, salinity affected the yield, physiology and nutritional quality of purslane. The impacts of LED spectral quality on purslane were only reflected by certain physiological and nutritional parameters.
Movilidades y agencia: ¿Cómo sortear el insilio?
Authors: Gatica Mónica , Casola Natalia 
Published: September 2023

El insilio constituye una experiencia colectiva que por su propia naturaleza ha quedado profundamente invisibilizada, negada hasta en su nominación. No sabemos cuántxs han sido las personas que en Argentina estuvieron en esa condición. No sabemos si alguna vez se han pensado como insialiadxs, ni qué reflexiones hicieron al respecto.
En este artículo buscamos abocarnos al análisis y reflexión que las experiencias de insilios suponen, abordando movilidades forzadas hacia el NE de Chubut durante el terrorismo de Estado. El recorte de una escala espacio temporal, se relaciona con el profundo interés de narrar la historia de lxs trabajadxs en el NE de Chubut, pero, a la vez, oficia como una decisión metodológica posible, que nos permite recortar el universo del insilio para observar qué elementos en común construyen la experiencia colectiva. A partir de tres entrevistas realizadas en distintos momentos buscamos probar el valor y las posibilidades que encierra el análisis propuesto y construir preguntas para un campo de estudios que todavía no ha sido suficientemente frecuentado.
Puerto Deseado, polo de la industria pesquera en Santa Cruz, Patagonia Argentina. Evolución de la rama y conflicto social
Authors: Martínez Susana , Huenul Víctor Hugo 
Published: September 2023

En julio de 2007 en Puerto Deseado (Santa Cruz, Argentina) trabajadores del sector pesquero protagonizaron un conflicto laboral que derivó en el ataque y quema de más de 10 plantas pesqueras situadas en la ciudad. Conflicto que puso el foco de atención respecto de la actividad y en particular de sus trabajadores.
La industria pesquera ligada a la exportación tuvo en la provincia un crecimiento sostenido desde la década de 1990, convirtiendo al puerto deseadense en el principal punto de operaciones de la flota de pesca de altura con la presencia de numerosas empresas propiedad de capitales extranjeros.
Este artículo es una primera aproximación a la cuestión de las luchas de los trabajadores de la pesca a la vez que pretende dar cuenta de la evolución de la actividad pesquera y los cambios operados en el sector en el marco de las transformaciones acontecidas en la estructura económica santacruceña a partir de la última década del siglo XX, al convertirse en hegemónicas las relaciones propias del capital financiero.
inicio de la actividad pesquera industrial en la Patagonia: un análisis con foco en Puerto Rawson, Chubut
Authors: Pérez Álvarez Gonzalo 
Published: September 2023

Describo y analizo el inicio de la actividad pesquera industrial en la Patagonia, reconstruyendo parte de la historia del desarrollo de esta rama económica en la región a partir de diversas fuentes (medios de prensa locales, documentos oficiales, estudios científicos, etc.). El punto de asiento clave de esta pesquería fue Puerto Rawson, en el noreste de Chubut.
Allí, desde mediados de la década del ’40 del siglo XX, comenzó a estructurarse una actividad pesquera con características industriales. Se registra la transformación generada por la dinamización de esta pesquería, a partir de la demanda del aceite vitamínico provisto por el hígado de cazón y luego por la captura de langostinos y otras especies.
Hacia el final del período analizado, la región empezó a transitar la implantación del modelo de polo desarrollista sobre su territorio, proceso en el cual la pesca no fue considerada un eje clave. La actividad quedó reducida a una lógica de enclave, dependiente de mercados extrarregionales y con extremas fluctuaciones según la presencia o escasez del recurso, sin que las políticas estatales generasen respuestas ante esos ciclos. Por ello describir, analizar y periodizar el inicio de esta rama económica en Patagonia aportará a pensar caminos para no repetir esas dinámicas.