bluuebunny/crossref_metadata_2025_split huggingface dataset:
Automatic Enhancement Preprocessing for Segmentation of Low Quality Cell Images
Authors: Kato Sota , Hotta Kazuhiro 
Published: January 2023

Abstract
We present a novel automatic preprocessing and ensemble learning technique for the segmentation of low quality cell images. Capturing cells with strong light is difficult because cells die by strong light. Therefore, microscopic images of cells tend to be of low quality, which is unsuitable for semantic segmentation. This study presents automatic enhancement preprocessing (AEP), which translates an input image into images that are easy to recognize using deep learning. AEP consists of two deep neural networks. The first network is for semantic segmentation, and the penultimate feature maps of the first network are used as filters to translate an input image into images that are easily classified by a convolutional neural network. The two networks are trained in an end-to-end manner, and preparing high-quality images is not required for translation. Additionally, we combined the segmentation results using a weighted ensemble learning approach called automatic weighted ensemble learning (AWEL). Because the second network outputs the segmentation results corresponding to each translated input image, we can aggregate multiple segmentation results by automatically determining suitable weights using AWEL. Experiments on two types of cell image datasets confirmed that AEP can translate low-quality cell images into images that are easy to segment and that segmentation accuracy improves using AWEL. Our code is available at https://github.com/usagisukisuki/AEP .
RNA-binding deficient TDP-43 drives cognitive decline in a mouse model of TDP-43 proteinopathy
Authors: Necarsulmer Julie , Simon Jeremy , Evangelista Baggio , Chen Youjun , Tian Xu , Nafees Sara , Gonzalez Ariana Marquez , Wang Ping , Ajit Deepa , Nikolova Viktoriya , Harper Kathryn , Ezzell Jennifer , Beltran Adriana , Moy Sheryl , Cohen Todd 
Published: January 2023

Abstract
TDP-43 proteinopathies including frontotemporal lobar dementia (FTLD) and amyotrophic lateral sclerosis (ALS) are neurodegenerative disorders characterized by aggregation and mislocalization of the nucleic-acid binding protein TDP-43 and subsequent neuronal dysfunction. Here, we developed an endogenous model of sporadic TDP-43 proteinopathy based on the principle that disease-associated TDP-43 acetylation at lysine 145 (K145) alters TDP-43 conformation, impairs RNA-binding capacity, and induces downstream mis-regulation of target genes. Expression of acetylation-mimic TDP-43K145Q resulted in stress-induced nuclear TDP-43 foci and loss-of-TDP-43-function in primary mouse and human induced pluripotent stem cell (hiPSC)-derived cortical neurons. Mice harboring the TDP-43K145Q mutation recapitulated key hallmarks of FTLD, including progressive TDP-43 phosphorylation and insolubility, TDP-43 mis-localization, transcriptomic and splicing alterations, and cognitive dysfunction. Our study supports a model in which TDP-43 acetylation drives neuronal dysfunction and cognitive decline through aberrant splicing and transcription of critical genes that regulate synaptic plasticity and stress response signaling. The neurodegenerative cascade initiated by TDP-43 acetylation recapitulates many aspects of FTLD and provides a new paradigm to further interrogate TDP-43 proteinopathies.
Irreversible loss in marine ecosystem habitability after a temperature overshoot
Authors: Santana-Falcón Yeray , Yamamoto Akitomo , Lenton Andrew , Jones Chris , Burger Friedrich A. , John Jasmin , Tjiputra Jerry , Schwinger Jörg , Kawamiya Michio , Frölicher Thomas , Ziehn Tilo , Seferian Roland 
Published: January 2023

Abstract
Anthropogenic warming of the oceans and associated deoxygenation are altering marine ecosystems. Current knowledge suggests that these changes might be reversible in the centennial timescale in the ocean surface and irreversible at deeper depth if global warming were to decline. However, knowledge on the persistence of their combined effects on marine ecosystems remains limited. Here we explore to what extent global warming will drive alterations on marine habitats by following the evolution of a metabolic index that captures the ecophysiological response of marine organisms to both changes in temperature and oxygen, through an idealised ramp-up ramp-down atmospheric CO2 concentration experiment. Using a multi-model approach, we find that changes in ocean temperature and oxygen drives a centuries-long irreversible loss of ~4% in the habitable volume of the upper 1000 m of the world ocean. These results suggest the combined effect of warming and deoxygenation will diminish the capability of the ocean to hold life far after recovering from a temperature overshoot.
The association between psychosocial factors and mental health symptoms in cervical spine pain with or without radiculopathy on health outcomes: a systematic review
Authors: Mansfield Michael , Thacker Mick , Taylor Joseph , Bannister Kirsty , Spahr Nicolas , Jong Stephanie T. , Smith Toby 
Published: January 2023

Abstract
Background

Neck pain, with or without radiculopathy, can have significant negative effects on physical and mental wellbeing. Mental health symptoms are known to worsen prognosis across a range of musculoskeletal conditions. Understanding the association between mental health symptoms and health outcomes in this population has not been established. Our aim was to systematically review the association between psychosocial factors and/or mental health symptoms on health outcomes in adults with neck pain, with or without radiculopathy.

Methods

A systematic review of published and unpublished literature databases was completed. Studies reporting mental health symptoms and health outcomes in adults with neck pain with or without radiculopathy were included. Due to significant clinical heterogeneity, a narrative synthesis was completed. Each outcome was assessed using GRADE.

Results

Twenty-three studies were included (N = 21,968 participants). Sixteen studies assessed neck pain only (N = 17,604 participants); seven studies assessed neck pain with radiculopathy (N = 4,364 participants). Depressive symptoms were associated with poorer health outcomes in people with neck pain and neck pain with radiculopathy. These findings were from seven low-quality studies, and an additional six studies reported no association. Low-quality evidence reported that distress and anxiety symptoms were associated with poorer health outcomes in people with neck pain and radiculopathy and very low-quality evidence showed this in people with neck pain only. Stress and higher job strain were negatively associated with poorer health outcomes measured by the presence of pain in two studies of very low quality.

Conclusions

There is a negative association of mental health symptoms and health outcomes in people with neck pain, with or without radiculopathy. These findings are based on a small number of highly heterogenous, low- and very low-quality studies. Clinicians should continue to utilise robust clinical reasoning when assessing the complex factors impacting a person’s presentation with neck pain with or without radiculopathy.
PROSPERO registration number: CRD42020169497
Interleukin-4 improved adipose-derived stem cells engraftment via interacting with fibro/adipogenic progenitors in dystrophic mice
Authors: Li Huan , Lin Jinfu , Wang Liang , He Ruojie , Li Jing , Chen Menglong , Zhang Weixi , Zhang Cheng 
Published: January 2023

Abstract
Adipose-derived stem cells (ADSC) therapy is a promising therapy for dystrophinopathy. Fibro/adipogenic progenitors (FAP) are important in regulating the myogenesis of muscle satellite cells and contribute to muscle fibrosis and adipocyte infiltration. The interleukin-4 (IL4) pathway is found to be a switcher regulating the functions of FAP. The interaction between FAP and engrafted cells has not yet been studied. We used a co-culture system to investigate the possible crosstalk between FAP of dystrophic mice and IL4-overexpressed ADSC (IL4-ADSC) and control ADSC. The systemic transplantation of IL4-ADSC and control ADSC was conducted in dystrophic mice for 16 weeks and motor function and molecular improvements of mice were evaluated. Overexpression of IL4 in ADSC significantly promoted terminal myogenesis in vitro with significant increased expression of Myogenin and MyHC. Through co-culture, we discovered that myoblasts derived from control ADSC promoted adipogenic and fibrogenic differentiation of FAP, but FAP did not significantly affect their myogenesis, while overexpression of IL4 in ADSC inhibited their myotube-dependent promotion of FAP differentiation but promoted FAP to support myogenesis. Dystrophic mice delivered with IL4-ADSC-derived myoblasts had a significant better motor ability, more engrafted cells with dystrophin expression, less muscle fibrosis, and intramuscular adipocytes and macrophage infiltration than mice delivered with control-ADSC-derived myoblasts. Our results revealed the importance of focusing on the crosstalk between engrafted cells and resident FAP in cell therapy and the positive therapeutic effect of IL4 administration combined with ADSC therapy in dystrophic mice.
Real-time monitoring of fast gas dynamics with a single-molecule resolution by frequency-comb-referenced plasmonic phase spectroscopy
Authors: Kim Young-Jin , Nguyen Anh , Lee Geon-Ho , Kim San , Shin Dong-Chel , Kim Dae-Hee , Park Jongkyoon , Choi Hak-Jong , Kim Seung-Woo , Kim Seungchul 
Published: January 2023

Abstract
Surface plasmon resonance (SPR) sensors are based on photon-excited surface charge density oscillations confined at metal-dielectric interfaces, which makes them highly sensitive to biological or chemical molecular bindings to functional metallic surfaces. Metal nanostructures further concentrate surface plasmons into a smaller area than the diffraction limit, thus strengthening photon-sample interactions. However, plasmonic sensors based on intensity detection provide limited resolution with long acquisition time owing to their high vulnerability to environmental and instrumental noises. Here, we demonstrate fast and precise detection of noble gas dynamics at single molecular resolution via frequency-comb-referenced plasmonic phase spectroscopy. The photon-sample interaction was enhanced by a factor of 3,600 than the physical sample thickness owing to plasmon resonance and thermophoresis-assisted optical confinement effects. By utilizing a sharp plasmonic phase slope and a high heterodyne information carrier, a small atomic-density modulation was clearly resolved at 5 Hz with a resolution of 0.06 Ar atoms per nano-hole (in 10− 11 RIU) in Allan deviation at 0.2 s; a faster motion up to 200 Hz was clearly resolved. This fast and precise sensing technique can enable the in-depth analysis of fast fluid dynamics with the utmost resolution for a better understanding of biomedical, chemical, and physical events and interactions.
Understanding Spatial Historical and Future Landslide Variation in Africa
Authors: Nahayo Lamek , Cui Peng , Yu Lei , Tan Rongzhi 
Published: January 2023

Abstract
The African natural landscape reshaping in search for housing, food and infrastructure development exposes the slope to failure. However, the entire African landslide characterization is still not well known due to limited studies covering the whole continent. The authors recognize this fact and conduct this study to present the historical African landslide susceptibility (1990–2020) and the 2050 predicted occurrence under urbanization practices. Literature identifies 26,211 recent landslides and high number is localized within same areas highlighted by the 2006–2017 NASA landslide inventory. For periodical landslide susceptibility mapping, rainfall, urbanization and LULC are selected as major drivers based on literature and inventory. Each of these factors’ historical maps are estimated to date (2022). These factors are combined with elevation, slope, aspects, curvature, distance to roads, distance to rivers, distance to faults, soil moisture, soil texture and lithology as of 2022 to estimate the current (2022) spatial landslide susceptibility. The Information Gain Ratio sensitivity analysis highlights urbanization (0.106), LULC (0.097), slope (0.091), elevation (0.088) and rainfall (0.083) as key landslide drivers. The Southern and Horn of Africa record above 80% of high and very high susceptibility classes. This 2022 susceptibility map is then predicted to 2050 and reclassified as that of 2050 urbanization (base map). The Southern, Eastern, Northern and Horn of Africa are landslide prone areas. This new study helps policy makers to ensure proper land planning and management practices for sustainable urbanization and lowering loss on human lives, damage on properties and environment.
Spatiotemporal immune atlas of the first clinical-grade,
gene-edited pig-to-human kidney xenotransplant
Authors: Porrett Paige , Cheung Matthew , Asiimwe Rebecca , Erman Elise , Fucile Christopher , Liu Shanrun , Sun Chiao-Wang , Hanumanthu Vidya , Pal Harish , Wright Emma , Ghajar-Rahimi Gelare , Epstein Daniel , Orandi Babak , Kumar Vineeta , Anderson Douglas , Greene Morgan , Bell Markayla , Yates Stefani , Moore Kyle , Lafontaine Jennifer , Killian John , Baker Gavin , Perry Jackson , Reed Rhiannon , Little Shawn , Rosenberg Alexander , George James , Locke Jayme 
Published: January 2023

Abstract
Pig-to-human xenotransplantation is rapidly approaching the clinical arena; however, it is unclear which immunomodulatory regimens will effectively control human immune responses to pig xenografts. We transplanted a gene-edited pig kidney into a brain-dead human recipient on pharmacologic immunosuppression and studied the human immune response to the xenograft using spatial transcriptomics and single-cell RNA sequencing. Human immune cells were uncommon in the porcine kidney cortex early after xenotransplantation and consisted of primarily myeloid cells. Both the porcine resident macrophages and human infiltrating macrophages expressed genes consistent with an alternatively activated, anti-inflammatory phenotype. No significant infiltration of human B or T cells into the porcine kidney xenograft was detected. Altogether, these findings provide proof of concept that conventional pharmacologic immunosuppression is sufficient to restrict infiltration of human immune cells into the xenograft early after compatible pig-to-human kidney xenotransplantation.
Blocking the CXCL1-CXCR2 axis enhances the effects of doxorubicin in HCC by remodeling the tumor microenvironment via the NF-κB/IL-1𝛽/CXCL1 signaling pathway
Authors: Zhao Huiyong , Wei Sheng , Zhou Dachen , Liu Yongfan , Guo Zicheng , Fang Chuibao , Pang Xiaoxi , Li Fei , Hou Hui , Cui Xiao 
Published: January 2023

Abstract
Inflammation is a core mechanism for oncogenesis. Chemokines act as important inflammation mediators in chronic inflammation and the tumor inflammatory response. However, limited information is known about chemokines in hepatocellular carcinoma (HCC), a disease that is almost entirely derived from chronic liver inflammation. Here, we explored the protumor effects of CXCL1, a commonly elevated inflammatory chemokine in cirrhosis, in HCC. This protumor feature was confirmed in clinical samples of human HCC. CXCL1 enhances tumorigenesis in the hepatic inflammatory microenvironment directly through tumor cells and indirectly through recruitment of macrophages. Increasing the number of macrophages in the tumor microenvironment (TME) promoted tumor cell Epithelial-mesenchymal transition (EMT) capacity and significantly elevated CXCL1 levels in the TME partly through NF-κB/IL-1𝛽 activation. To investigate the potential therapeutic value of CXCL1 in HCC with inflammatory background, blocking CXCL1 and blocking CXCL1 combined with the chemotherapy agent doxorubicin (DOX), which aimes to reshape the TME, were administered. It has been shown that blocking CXCL1-CXCR2 inhibits tumor progression and reduces macrophage recruitment in the TME. The combination regimen has been shown to have a synchronous effect in HCC by reducing pro-tumor macrophages in the TME and suppressing tumor cell progression. This provides insight into therapeutic strategies for treating HCC patients with high CXCL1 expression.
Participatory landscape sustainability assessment: where do we stand? A systematic literature review
Authors: Moreau Clémence , Blanco Julien , Randriamalala Josoa , Laques Anne-Elisabeth , Carrière Stéphanie M. 
Published: January 2023

Abstract
Context

In line with interdisciplinary and transdisciplinary principles of Sustainability Science, Participatory Landscape Sustainability Assessment (PLSA) studies are developing. These studies make the paradoxical promises to standardize observations to obtain robust and comparable data, while including a diversity of case studies, stakeholders and viewpoints.

Objective

The purpose of this paper is to highlight the diversity of perspectives on PLSA, arguing that the lack of coherent framing and a poor substantive theorization can limit the development of PLSA studies.

Methods

We conducted a systematic literature review on a corpus of 425 publications by combining bibliographic mapping on the full corpus and a content analysis on a sub-corpus of 138 full-texts.

Results

Our results showed that (i) this field is at the crossroads between ecology, landscape planning and sociocultural approaches, (ii) indicators combine on average 4.7 categories of sustainability, but are most applied at a local level and in a snapshot perspective, (iii) stakeholders are rarely involved in the choice of indicators (28.9% of studies) or in the assessment design (7.2%), and are mostly only asked to populate pre-identified indicators (63.9%), and (iv) viewpoint diversity is taken into consideration mainly by using indicators (67.3%) rather than by promoting deliberation (39.8%). In sum, we distinguish the participation-oriented approach, the contributive approach (anchored in positivism) and the collaborative approach (anchored in constructivism).

Conclusion

We advocate that future studies should be more explicit on the perspective they adopt in order to improve consistence between PLSA objectives, methods, and theoretical anchorages. This clarification might ultimately avoid confusion about the different regimes of participation and their respective contributions to Landscape Sustainability Science.
The protocol of DECO-MOM: a clinical trial testing the effectiveness of a mobile application for an environmental health intervention among pregnant women
Authors: Kim Hyun Kyoung 
Published: January 2023

Abstract
Background: Environmental toxins are particularly harmful to pregnant women and their fetuses due to the long-term effects of these toxins on children after birth. Environmental health behaviors can prevent and protect mothers and their babies’ environmental health.
Methods/Design: This study presents a protocol to examine the effect of a mobile application named “DECO-MOM” (DEep Ecology-MOM) for pregnant women. This application contains content related to environmental perceptions and behaviors according to the revised protection motivation theory. The mobile intervention will be implemented for 4 weeks for 40 pregnant women with a gestational age in the first trimester. As the control group, 40 pregnant women will be recruited at public healthcare centers in Chuncheon and Gongju cities in South Korea. The hypothesis is that the experimental group will have higher scores for environmental perceptions and behaviors than the control group. An online survey administered before and after the intervention will contain outcome parameters including depression, stress, quality of life, e-learning satisfaction, environmental perceptions, and environmental behaviors.
Discussion: This study will elaborate a guide for an intervention to enhance the effects of the mobile application on strengthening environmental health perceptions, behaviors, e-learning satisfaction, and quality of life and curtailing depression and stress among pregnant women.
Trial registration: Trial registration Number: KCT0007725
Comparison of statistical methods used to meta-analyse results from interrupted time series studies: an empirical study
Authors: Korevaar Elizabeth , Turner Simon L , Forbes Andrew B , Karahalios Amalia , Taljaard Monica , McKenzie Joanne E 
Published: January 2023

Abstract
Background
The Interrupted Time Series (ITS) is a robust design for evaluating public health and policy interventions or exposures when randomisation is infeasible. Several statistical methods are available for the analysis and meta-analysis of ITS studies. We sought to empirically compare available methods when applied to real-world ITS data.
Methods
We sourced ITS data from published meta-analyses to create an online data repository. Each dataset was re-analysed using two ITS estimation methods. The level- and slope-change effect estimates (and standard errors) were calculated and combined using fixed-effect and four random-effects meta-analysis methods. We examined differences in meta-analytic level- and slope-change estimates, their 95% confidence intervals, p-values, and estimates of heterogeneity across the statistical methods.
Results
Of 40 eligible meta-analyses, data from 17 meta-analyses including 283 ITS studies were obtained and analysed. We found that on average, the meta-analytic effect estimates, their standard errors and between-study variances were not sensitive to meta-analysis method choice, irrespective of the ITS analysis method. However, confidence interval widths and p-values for the meta-analytic effect estimates varied depending on the choice of confidence interval method and ITS analysis method.
Conclusions
The meta-analysis effect estimates, their standard errors and between-study variance estimates were minimally impacted by ITS analysis and meta-analysis method choice. However, the confidence interval widths and p-values could vary according to the statistical method, which may impact interpretations and conclusions of a meta-analysis. This empirical study, in conjunction with evidence from numerical simulation, allows for a more complete understanding of which methods should be used in different scenarios.