bluuebunny/crossref_metadata_2025_split huggingface dataset:
Impact of hepatitis B virus infection on IVF/ICSI-assisted pregnancy outcomes in women: a propensity score-matched cohort study
Authors: Suqin Zhu , Wenwen Jiang , Rongshan Li , Yan Sun , Xiaojing Chen , Beihong Zheng 
Published: June 2023

Abstract
Objective To explore the impact of hepatitis B virus infection on In Vitro Fertilization/ Intracytoplasmic Sperm Injection (IVF/ ICSI)-assisted pregnancy outcomes in women.
Methods A retrospective cohort study design was used. A retrospective analysis was performed on the clinical data of 3455 cycles of infertile couples who underwent fresh embryo transfer with IVF/ICSI-ET assisted pregnancy for the first time in the Reproductive Center of Fujian Maternal and Child Health Hospital from January 2018 to December 2020, and the male did not carry HBV virus. Patients were divided into HBV group (n=811) and non-HBV group (n=2644) based on whether the women carried HBV virus or not. Propensity score matching method (PSM) was used to perform 1:1 matching for the HBV group to the non-HBV group with relatively balanced covariates, and the final HBV and non-HBV groups were both 805 cycles. The baseline levels of the two matched groups were consistent, and the laboratory and clinical outcomes of IVF/ICSI assisted pregnancy were compared between the two groups.
Results Baseline levels such as male age, female age, body mass index, basal follicle-stimulating hormone (FSH), basal luteinizing hormone (LH), basal estrogen (E2), anti-Mullerian hormone (AMH), basal antral follicle count, years of infertility, types of infertility, causes of infertility, ovulation induction regimen, the duration of gonadotropin (Gn), Gn dose, E2 level on HCG day, progesterone level on HCG day, endometrial thickness on HCG day, method of insemination(IVF/ICSI), number of transferred embryos and type of transferred embryos were relatively consistent in the two groups after PSM, with no significant differences (P > 0.05). There were no statistically significant differences in egg maturation rate (52.89% vs 53.47%), high-quality embryo rate (57.76% vs 58.51%), blastocyst formation rate (63.48% vs 63.56%) and clinical pregnancy rate (44.47% vs 44.84%), live birth rate (32.92% vs 34.91%), perinatal complications and birth fetal weight between the two groups (P > 0.05 ). However, the preterm birth rate was significantly higher in the HBV-infected group compared to the non-HBV group, with a statistically significant difference between the two groups (15.85% vs. 9.96%, P=0.04).
Conclusion HBV infection in women increased the risk of preterm delivery in IVF/ICSI-assisted pregnancy, but pregnancy and live birth rates were comparable to those of non-HBV-infected patients and were not significantly associated with poor obstetric and neonatal outcomes.
Noise-robust, physical genuine deconvolution algorithm for fluorescence microscopy enabled by multi-resolution analysis
Authors: Hou Yiwei , Wang Wenyi , Fu Yunzhe , Ge Xichuan , Li Meiqi , Xi Peng 
Published: June 2023

Abstract
Fluorescence microscopic imaging is essentially a convolution process, with noise added during the photon conversion or readout process, confining imaging speed, duration, and resolution. Aiming at recovering biological information from the strong noise level to improve these key parameters, here we propose a multi-resolution analysis (MRA) approach to extract the two main characteristics of fluorescence images: (1) high contrast across the edge, and (2) high continuity along the edge. By regularizing the framelet and curvelet domain sparsity, we develop MRA and DeepMRA deconvolution algorithms, which generally improve the signal-to-noise ratio (SNR) by ~10 dB higher than state-of-the-art variance-based penalties, and can provide more than two-fold physical resolution enhancement without the artifact-prone Richardson-Lucy process. Our methods can accomplish more challenging imaging tasks in various fluorescence microscopies, such as wide-field, confocal, light-sheet, structured illumination microscopy (SIM), and stimulated excitation depletion (STED) microscopy.
Genetic relatedness of resistant toxigenic Vibrio cholerae from wastewater Irrigated vegetables from two major produce regions.
Authors: Abakpa Grace , Umoh Veronica , Sijam Kamaruzaman , Dashen Micheal 
Published: June 2023

Abstract
Nigeria suffers episodes of cholera outbreak every year often starting from the northern part of the country. The infection is often linked to contaminated food, water and poor hygiene. Vegetables are often irrigated with untreated wastewater in the country. This study aimed at assessing the presence of Vibrio cholerae, their virulence, resistant gene profiles and further elucidated their genetic relationships using PCR-based fingerprinting method; Enterobacterial repetitive intragenic consensus Polymerase Chain Reaction (ERIC-PCR) from two major produce regions in Nigeria. Four hundred and forty samples including wastewater used for irrigation, irrigated vegetables and manure treated soils were analyzed. Isolates were characterized using conventional and molecular techniques. Strains were serotyped, assayed for their toxigenic potentials and resistance to antimicrobial agents. Twenty-five of the samples (5.7%) were positive for Vibrio cholerae, of which 10.2% were detected from soil/manure samples. All strains were found to possess the ctxA gene and exhibited multiple drug resistance. Genotyping by ERIC-PCR of genomic DNA from 15 representative strains obtained resulted in the amplification of multiple fragments of DNA in sizes ranging from 150bp to 1kb. Cluster analysis at a coefficient of 0.62 grouped the strains into six different groups; five clusters and one singleton in simple-match (Sm) similarity group. Strains from vegetables and environmental samples were found clustered together. To the best of our knowledge it is the first report of microbial source tracking and genotypic relatedness of Vibrio cholerae from irrigated vegetables in the country. The need for continuous monitoring and surveillance of potential risk routes in the environment cannot be over emphasized.
Risk Factors and Metabolomics of Mild Cognitive Impairment in Type 2 Diabetes Mellitus
Authors: Jiang Xiao , Luo Tao , Xu Ning , Zhao Xinyu , Zhong CongChao , Xie Xingjie , He Mengfan , Bian XiaoLong , Liu Haixia 
Published: June 2023

Abstract
Objective: This study aimed to explore the controllable risk factors, metabolic characteristics and potential biomarkers of T2DM-MCI and to provide potential proof for the diagnosis, prevention and treatment of cognitive impairment in T2DM patients.
Methods: 103 T2DM patients who were hospitalized in the Endocrinology Department of the Second Hospital of Dalian Medical University were included. MoCA was used to evaluate the cognitive function of all subjects. 50 patients were included in T2DM-MCI group (MoCA score <26 points), and 53 subjects were in T2DM-NCI group (MoCA score ≥ 26 points). Serum samples of subjects were collected, and metabolomics data was collected by UHPLC-MS. The serum samples of 12 subjects were excluded due to poor quality, 47 subjects were in the T2DM-MCI group, 44 cases in T2DM-NCI group were used to analyze the differentially expressed small molecule metabolites, metabolics pathway and the potential specific biomarkers.
Results: Comparison between T2DM-MCI group and T2DM-NCI group, years of education, history of insulin application, and insulin resistance index, insulin-like growth factor-binding protein-3, and creatinine were significantly different between groups. Further binary logistic regression analysis of the variables showed low educational level and low serum insulin-like growth factor-binding protein-3 was an independent risk factor for T2DM-MCI. Metabolomics analysis showed that 10 metabolites were differentially expressed between T2DM-MCI group and T2DM-NCI group (P<0.05 and FDR<0.05, VIP>1.5). KEGG enrichment pathway reveals that fatty acid degradation is the most significant. ROC shows that LPC 18:0 has a greater diagnostic efficiency.
Conclusion: This study suggests that T2DM patients with low education levels, history of insulin use, high insulin resistance, low serum insulin-like growth factor binding protein-3, and low creatinine values are more susceptible to MCI. Short years of education and low serum insulin-like growth factor binding protein-3 levels are independent risk factors for MCI in combination with T2DM. There were significant differences in serum metabolites between T2DM-MCI and T2DM-NCI. Abnormal lipid metabolism plays an important role in the development of cognitive impairment in T2DM patients. LPC 18:0 can effectively differentiate T2DM-MCI and T2DM-NCI, expected to identify cognitive impairment in T2DM patients at an early stage.
Graphene-Fullerene Heterostructures as Robust and Flexible Nanomechanical Bits
Authors: Xue Yixuan , Park Harold S. , Jiang Jin-Wu 
Published: June 2023

Abstract
Electrical computers have revolutionized society over the past several decades, but questions have remained about their ability to perform in extreme environments. This has motivated the recent surge of interest in developing mechanical computing platforms at all length scales, including the nanoscale, in which traditional electrical computers are augmented with mechanical ones. However, most proposed nanomechanical bits are volatile memory bits based on the dynamic response of nanomechanical resonators, and as such there is a need to develop robust and reprogrammable entirely non-volatile nanomechanical bits. Here, we exploit the multiple quasi-stable configurations of the graphene/fullerene/graphene (GFG) van der Waals heterostructure to work as a novel non-volatile nanomechanical bit. The GFG heterostructure is unique and robust in that it can return to its initial state without further mechanical input, it can be used for logic functions at relevant operating temperatures through simple application of uniaxial strain, it can exhibit reprogrammability between five basic logic gates (NOT, AND, NAND, OR, NOR) by varying the magnitude or direction of applied strain, and it can be used to represent combinatorial logic through full and half adders. These findings provide a new opportunity to develop mechanical computers based on the large class of nanomaterials.
The use of oxygen-ozone therapy to improve the effectiveness of antibiotic treatment on infected arthroplasty: protocol for a superiority, open-label, multicentre, randomized, parallel trial
Authors: Cascini Fidelia , Franzini Marianno , Andreoli Arnaldo , Manzotti Alfonso , Cadeddu Chiara , Quaranta Gianluigi , Gentili Andrea , Ricciardi Walter 
Published: June 2023

AbstractIntroduction.Surgical site infections still remain a major public health challenge and have become an increasing universal risk, especially for implantation of orthopaedic devices. Ozone therapy has been extensively used and studied for decades across various potential medical applications and has provided consistent effects with minimal side effects. This study aims to determine the superiority of oxygen-ozone therapy in combination with oral antibiotic therapy in patients with wound infections after an orthopaedic device’s implantation, when compared to antibiotic therapy alone.Methods and analysis.This is an open-label, multicentre, randomized, parallel-group study to assess efficacy and safety of oxygen-ozone therapy in combination with oral antibiotic therapy to treat infections following an orthopaedic implant. Patients (n=186) will be recruited from 12 Italian hospitals and studied for 7 weeks. All will be randomized and placed into either the (1) intervention: oxygen-ozone therapy two to three times a week for 6 weeks (for a maximum of 15 sessions) simultaneously with an appropriate oral antibiotic therapy prescribed at baseline; or (2) control: oral antibiotic therapy prescribed at baseline. Primary outcome is the efficacy and superiority of the treatment (ozone and oral antibiotic therapies); secondary outcomes include the resolution of signs/symptoms, modifications in lesion size and treatment’s safety and tolerability.Ethics and dissemination.This study will be reviewed and approved by the Independent Ethics Committee (IEC) of all participating centres prior to starting. Findings will be disseminated via online and print media, events, and peer-reviewed journals.Trial Registration Number:NCT04787575
Breastfeeding Practices of Refugee, Migrant and Asylum seekers Mothers in Portugal and Sustainable Development Goals
Authors: Lopes Ana Cláudia , Lousada Marisa 
Published: June 2023

Abstract
Background
This study aimed to analyze the perspectives of refugee and migrant mothers in Portugal about breastfeeding and compare their practices with globally available data and those of the native countries while considering the Sustainable Development Goals (SDGs).
Methods
A Sociodemographic questionnaire and a Breastfeeding Knowledge, Attitude and Beliefs, and Behavior questionnaire were used to gather information regarding baseline breastfeeding knowledge, attitude and beliefs, and behaviour towards breastfeeding.
Results
Only 40% of the mothers received antenatal counselling regarding the benefits and management of breastfeeding. Of the 20 responses, 10 (50%) mothers were found to have fair breastfeeding knowledge, 16 (80%) had fair attitudes and beliefs, and 12 (60%) had fair breastfeeding practices. Correlation analysis indicated a positive correlation between mothers’ breastfeeding attitudes (r = .531, p < 0.05) and their breastfeeding knowledge. There was no statistically significant correlation between the mothers’ breastfeeding attitudes, beliefs, and practices.
Conclusions
The findings of this study suggest that healthy breastfeeding behaviours can be stimulated by receiving proper counselling from health professionals. Countries must focus on improving breastfeeding practices, as they still fail to do all they can to promote, protect, and support globally. Universal interventions are necessary to improve breastfeeding, regardless of migrant or refugee status.
∆Np63α inhibits Rac1 activation and cancer cell invasion through suppression of PREX1
Authors: Aljagthmi Amjad , Hira Akshay , Zhang Jin , Cooke Mariana , Kazanietz Marcelo , Kadakia Madhavi 
Published: June 2023

Abstract
ΔNp63α, a member of the p53 family of transcription factors, plays a critical role in maintaining the proliferative potential of stem cells in the stratified epithelium. Although ΔNp63α is considered an oncogene and is frequently overexpressed in carcinoma cells, loss of ΔNp63α expression is associated with increased cancer invasion and metastasis. We recently identified a ΔNp63α/miR-320a/PKCγ signaling axis that regulates cancer cell invasion by inhibiting phosphorylation of the small GTPase Rac1, a master switch of cell motility that positively regulates cell invasion in multiple human cancers. In this study, we identified a novel mechanism by which ΔNp63α negatively regulates Rac1 activity, by inhibiting the expression of the Rac-specific Guanine Exchange Factor PREX1. ΔNp63α silencing in multiple squamous cell carcinoma cell lines leads to increased Rac1 activation, which is abrogated by treatment with Rac1 inhibitor NSC23766. Furthermore, ΔNp63α binds to the PREX1 promoter, leading to reduced PREX1 transcript and protein levels. Using a Rac-GEF activation assay, we also showed that ΔNp63α reduces the levels of active PREX1. The inhibition of the PREX1-Rac1 signaling axis by ΔNp63α leads to impaired cell invasion, thus establishing the functional relevance of this signaling axis. Our results elucidated a novel molecular mechanism by which ΔNp63α negatively affects cancer cell invasion and identifies the ΔNp63α/Rac1 axis as a potential target for metastatic cancers.
Landau's theorem for harmonic Bloch functions on simply connected domains
Authors: Allu Vasudevarao , Halder Himadri 
Published: June 2023

Abstract
For $\alpha \in (0,\infty)$, let $\mathcal{B}_{\mathcal{H},\Omega}(\alpha)$ denote the class of harmonic $\alpha$-Bloch mappings on a proper simply connected domain $\Omega \subseteq \mathbb{C}$. In this article, we study coefficient estimates for harmonic $\alpha$-Bloch mappings on a proper simply connected domain containing the unit disk. Furthermore, we establish the Landau's theorem and coefficient estimates for the harmonic $\alpha$-Bloch space $\mathcal{B}_{\mathcal{H},\Omega _{\gamma}}(\alpha)$ on the shifted disk $\Omega_{\gamma}$, where $$ \Omega_{\gamma}:=\bigg\{z\in\mathbb{C} : \bigg|z+\frac{\gamma}{1-\gamma}\bigg|<\frac{1}{1-\gamma}\bigg\} $$ and $0 \leq \gamma <1. $
Effect of different doses of chemical and physical mutagen on genetic variability and character association in M1 and M2 generation of Momordical balsamina L.
Authors: BAMMANAKATTI SHARAVATI M , Rathod Vijayakumar , Gasti Vilas , Naik K Ramachandra , Chavan Mukesh , Evoor Shashikant , A Prashantha , N. Mahantesha Naika B. 
Published: June 2023

Abstract

Momordica balsaminaL.commonly known as the Balsam apple is an under-exploited cucurbit vegetable occurs wild type in nature which offers various dietary and nutraceutical properties. It is an annual to perennial, tendril-bearing herb, native to tropical regions of Africa. Since the crop occurs in wild forms, the variation exists among the genotype is limited. For the improvement of plant characteristics in diverse crops, mutation breeding has been extensively applied. In the present study, treated population was evaluated to study the extent of genetic variability and character association on growth and yield parameters in M1and M2generation ofMomordica balsaminaL. The results revealed that high heritability was exhibited by vine length at harvest, number of branches per plant at harvest, stem diameter, leaf area, number of fruits per plant, fruit girth, lycopene content, β-carotene content and ascorbic acid content. As per the character association studies,fruit yield per plant showed highly significant and positive association with number of fruits per plant, fruit weight, fruit length and days to first flowering. Whereas, highly significant but negative association was revealed by number of branches per plant at harvest, stem diameter and days to 50 per cent flowering among mutant population. Whereas, vine length at harvest, number of fruits per plant, fruit weight, fruit length and fruit girth recorded highest positive direct effect on fruit yield per plant. While, number of branches per plant at harvest, stem diameter and days to 50 per cent flowering exerted negative direct effect on fruit yield per plant.
Changes in transcription of genes encoding NAD-dependent enzymes and mitochondrial proteins in Alzheimer’s disease animal model. Indication of early targets in neuroprotection
Authors: Żulińska S , Wencel PL , Wieczorek Iga , Strosznajder Robert P , Strosznajder Joanna Benigna 
Published: June 2023

Abstract
Oxidative stress and disturbances of mitochondria function in the brain play a crucial role in Alzheimer’s disease (AD). However, little is known about these changes at the early stages of AD. This study aimed to determine the expression of genes encoding superoxide dismutase’s (SOD1, SOD2), Sirtuins (SIRTs) and poly (ADP-ribose) polymerases (PARPs). Moreover, transcription of genes of electron transport complexes (ETC) and proteins of mitochondrial biogenesis in the brain cortex of 3-, 6- and 12-month-old transgenic AD mice was analyzed. Using quantitative qPCR and immunochemical methods, we demonstrated significant decreases in mRNA of Sod2, Sirt1 and Parp1 in the 3-month-old and upregulation of Parp1 in the 6-month-old AD mice. Although levels of mRNA encoding ETC, respiratory complexes subunits (I-II) were negligibly altered, the mRNA mt-CytB and mt-Co1 (complex III, IV) was increased in 12- and 6-month-old AD brains, respectively. These changes were linked to lower cytochrome C oxidase activity in 3- and significantly in 6-month-old AD mice. Several genes involved in mitochondria biogenesis, such as Nrf1, Nrf2 and Tfam, were upregulated in the 3- and 6-month-old AD Tg brain. However, in 12-month-old AD mice, transcription of genes encoding NRF2, PPAR-α, and PGC1-α was significantly downregulated. In summary, our data identified significant changes in gene expression of Sod2, Sirt1 and Parp1 at an early age (3–6 month-old AD mice) and Nrf2, Ppargc1, Ppar-α at the later stage of AD mice. Recognizing these alterations earlier may be important in providing potential therapeutic targets for delaying the progression of pathology in AD.
Unraveling Extreme Weather Impacts on Air Transportation and Passenger Delays using Location-based Data
Authors: Hsu Chia-Wei , Liu Chenyue , LIU Zhewei , Mostafavi Ali 
Published: June 2023

Abstract
Extreme weather poses significant threats to air transportation systems, causing flight rerouting and cancellations, as well as passenger travel delays. With the growing frequency of extreme weather hazards, it is essential to understand the extent to which disruptions in flights and subsequent cancellations impact passenger delays. This study focuses on quantifying the impacts of a recent extreme weather event (2022 Winter Storm Elliott) on the U.S. air transportation system by investigating passenger delays measured based on dwell time at airports using privacy-preserving location-based datasets. The study determines total dwell time and dwell time per anonymized user at airports during the extreme weather event and computes the impact based on changes in values compared to the same period in the previous year. The results show that the storm event caused passengers significant delays, as characterized by a substantial increase in airport dwell time. Factor analysis shows that airports with a greater passenger flow and a greater portion of flights from decentralized airlines aggravated passengers delays during the winter storm. The vulnerability of airports was mainly due to the direct storm exposure, and the influence of network cascading impacts were limited. The findings of this study provide novel insights and quantification of the extent of extreme weather impacts on air transportation at individual airports and national levels. These outcomes could inform airport owners and operators, as well as airlines, about the extent of vulnerability and provide useful information for weather-related risk assessment of air transportation systems.