bluuebunny/crossref_metadata_2025_split huggingface dataset:
Angie-LAMP for diagnosis of human eosinophilic meningitis using dog as proxy: A LAMP assay for Angiostrongylus cantonensis DNA in cerebrospinal fluid
Authors: Baláž Vojtech , Rivory Phoebe , Hayward Douglas , Jaensch Susan , Malik Richard , Lee Rogan , Modrý David , Šlapeta Jan 
Published: May 2023

Background
Angiostrongylus cantonensis (rat lungworm) is recognised as the leading cause of human eosinophilic meningitis, a serious condition observed when nematode larvae migrate through the CNS. Canine Neural Angiostrongyliasis (CNA) is the analogous disease in dogs. Both humans and dogs are accidental hosts, and a rapid diagnosis is warranted. A highly sensitive PCR based assay is available but often not readily accessible in many jurisdictions. An alternative DNA amplification assay that would further improve accessibility is needed. This study aimed to assess the diagnostic utility of a newly designed LAMP assay to detect DNA of globally distributed and invasive A. cantonensis and Angiostrongylus mackerrasae, the other neurotropic Angiostrongylus species, which is native to Australia.

Methodology/Principal findings
Cerebrospinal fluid (CSF) from dogs with a presumptive diagnosis of A. cantonensis infection (2020–2022) were received for confirmatory laboratory testing and processed for DNA isolation and ultrasensitive Angiostrongylus qPCR targeting AcanR3390. A newly designed LAMP assay targeting the same gene target was directly compared to the reference ultrasensitive qPCR in a diagnostic laboratory setting to determine the presence of A. cantonensis DNA to diagnose CNA. The LAMP assay (Angie-LAMP) allowed the sensitive detection of A. cantonensis DNA from archived DNA specimens (Kappa = 0.81, 95%CI 0.69–0.92; n = 93) and rapid single-step lysis of archived CSF samples (Kappa = 0.77, 95%CI 0.59–0.94; n = 52). Only A. cantonensis DNA was detected in canine CSF samples, and co-infection with A. mackerrasae using amplicon deep sequencing (ITS-2 rDNA) was not demonstrated. Both SYD.1 and AC13 haplotypes were detected using sequencing of partial cox1.

Conclusions/Significance
The Angie-LAMP assay is a useful molecular tool for detecting Angiostrongylus DNA in canine CSF and performs comparably to a laboratory Angiostrongylus qPCR. Adaptation of single-step sample lysis improved potential applicability for diagnosis of angiostrongyliasis in a clinical setting for dogs and by extension, to humans.
Quality of life in adults with Down syndrome: A mixed methods systematic review
Authors: Ijezie Ogochukwu Ann , Healy Jane , Davies Philip , Balaguer-Ballester Emili , Heaslip Vanessa 
Published: May 2023

Background
As the life expectancy of adults (aged ≥ 18 years) with Down syndrome increases for a plethora of reasons including recognition of rights, access, and technological and medical advances, there is a need to collate evidence about their quality of life.

Objective
Using Schalock and Verdugo’s multidimensional quality of life assessment model, this systematic review aimed to identify, synthesise and integrate the quantitative and qualitative evidence on quality of life in adults with Down syndrome via self-and proxy-reporting.

Methods
Five databases were systematically searched: MEDLINE, CINAHL, PsycINFO, Scopus, and Web of Science to identify relevant articles published between 1980 and 2022 along with grey literature and reference lists from relevant studies. A mixed methods systematic review was performed according to the Joanna Briggs Institute methodology using the convergent integrated approach. The review followed the Preferred Reporting Items for Systematic Reviews and Meta-Analyses guidelines.

Results
Thirty-nine studies were included: 20 quantitative, 17 qualitative, and 2 mixed methods studies. The synthesised findings were grouped into the 8 core domains of quality of life: personal development, self-determination, interpersonal relations, social inclusion, rights, emotional, physical and material well-being. Of the 39 studies, 30 (76.92%) reported on emotional well-being and 10 (25.64%) on rights. Only 7 (17.94%) studies reported that adults with Down syndrome have a good quality of life centred around self-determination and interpersonal relations. Most adults with Down syndrome wanted to become more independent, have relationships, participate in the community, and exercise their human rights. Self-reported quality of life from adults with Down syndrome was rated higher than proxy reported quality of life. Discrepancies in quality of life instruments were discovered.

Conclusion
This review highlighted the need for a better systematic approach to improving the quality of life in adults with Down syndrome in targeted areas. Future research is required to evaluate self-and proxy-reporting methods and culture-specific quality of life instruments that are more appropriate for adults with Down syndrome. In addition, further studies should consider including digital assistive technologies to obtain self-reported quality of life data in adults with Down syndrome.

International prospective register of systematic reviews registration number
CRD42019140056.
A gamificação com a plataforma wordwall como estratégia de aprendizagem para o ensino de matemática
Authors: Teófilo de Sousa Renata , Ferreira de Azevedo Italândia , Régis Vieira Alves Francisco 
Published: May 2023

Este trabalho traz os resultados de uma oficina realizada com os bolsistas do Programa Institucional de Bolsas de Iniciação à Docência, do curso de Licenciatura em Matemática da Universidade Estadual do Ceará, tratando sobre a gamificação no ensino de Matemática e apontando a relevância de sua incorporação à metodologia docente. O objetivo deste trabalho foi construir um conjunto de jogos didáticos direcionados à gamificação de componentes curriculares da disciplina de Matemática utilizando a plataforma Wordwall, com base em um planejamento de aula realizado pelos bolsistas, visando agregar o uso de tecnologias no ensino à sua formação acadêmica. A metodologia tem como natureza uma pesquisa exploratória, do tipo pesquisa-ação, ali os sujeitos participaram de forma ativa de uma oficina, onde foi apresentada a plataforma, sua dinâmica, materiais e formatos disponíveis como recurso ao professor. A partir do primeiro contato com o Wordwall, os bolsistas realizaram como atividade um planejamento de aula com uso da gamificação com o Wordwall, utilizando como temas componentes curriculares da disciplina de Matemática. Como resultados, traz-se um conjunto de planejamentos com possibilidades de aplicação em sala de aula, bem como as impressões dos bolsistas sobre a experiência vivenciada. Por fim, constata-se que existem poucas pesquisas sobre o tema gamificação com uso da plataforma Wordwall, sendo um campo vasto a ser explorado por pesquisadores da área, dado o potencial que esta estratégia de aprendizagem ativa pode desenvolver nos alunos.
Desenvolvendo conceitos e atitudes sobre a interdisciplinaridade: estratégia didática pelo uso do jogo tangram para a formação inicial de professores de ciências
Authors: Goldschmidt Andréa Inês , Oestreich Laura , Breunig Eduarda , Gutler Paim Mathes 
Published: May 2023

Este artigo teve por objetivo desenvolver uma estratégia de ensino que contribuísse para o ensino e a aprendizagem de conceitos sobre a interdisciplinaridade e para o reconhecimento de atitudes e habilidades necessárias nas ações a serem refletidas já na formação docente inicial. Para tanto, utilizou-se o jogo milenar Tangram como base, no intuito de desafiar a melhor compreensão dos conceitos relacionados ao conteúdo abordado, aplicado com trinta alunos em formação inicial docente, em ciências, em uma disciplina da área de Educação. Diversas analogias foram construídas a partir do jogo e relacionadas à conceituação dos termos interdisciplinaridade, multidisciplinaridade, pluridisciplinaridade e transdisciplinaridade. Igualmente, foram discutidos os comportamentos evidenciados durante a atividade, os desafios, as dificuldades e as atitudes necessárias para se pensar em práticas interdisciplinares no ambiente escolar. A validação da proposta se deu a partir e um questionário semiestruturado, respondido individualmente e analisado por Análise de Conteúdo. Conforme os resultados obtidos, a estratégia proposta se mostrou adequada para trabalhar questões relacionadas à interdisciplinaridade, tanto conceitos, quanto atitudes e habilidades, itens necessários para a consolidação de práticas interdisciplinares dentro da realidade escolar.
Testing and Profiling Athletes: Recommendations for Test Selection, Implementation, and Maximizing Information
Authors: Weakley Jonathon , Black Georgia , McLaren Shaun , Scantlebury Sean , Suchomel Timothy J. , McMahon Eric , Watts David , Read Dale B. 
Published: May 2023

ABSTRACT
Understanding the physical qualities of athletes can lead to improved training prescription, monitoring, and ranking. Consequently, testing and profiling athletes is an important aspect of strength and conditioning. However, results can often be difficult to interpret because of the wide range of available tests and outcome variables, the diverse forms of technology used, and the varying levels of standardization implemented. Furthermore, physical qualities can easily be misrepresented without careful consideration if fundamental scientific principles are not followed. This review discusses how to develop impactful testing batteries so that practitioners can maximize their understanding of athletic development while helping to monitor changes in performance to better individualize and support training. It also provides recommendations on the selection of tests and their outcome measures; considerations for the proper interpretation, setup, and standardization of testing protocols; methods to maximize testing information; and techniques to enhance visualization and interpretation.
Resident Communication With Patients and Families Preferring Languages Other Than English
Authors: Rojas Christina R. , Coffin Alyssa , Taylor April , Ortiz Priscilla , Jenicek Gabriela , Hart Jessica , Callahan James M. , Shaw Kathy N. 
Published: May 2023

OBJECTIVES
Patients and families preferring languages other than English (LOE) often experience inequitable communication with their health care providers, including the underutilization of professional interpretation. This study had 2 aims: to characterize resident-perceived communication with families preferring LOE and to evaluate the impact of language preference on frequency of resident interactions with hospitalized patients and families.

METHODS
This was a cross-sectional study at a quaternary care children’s hospital. We developed a questionnaire for residents regarding their interactions with patients preferring LOE. We concurrently developed a communication tracking tool to measure the frequency of resident communication events with hospitalized patients. Data were analyzed with logistic and Poisson regression models.

RESULTS
Questionnaire results demonstrated a high level of resident comfort with interpretation, though more than 30% of residents reported “sometimes” or “usually” communicating with families preferring LOE without appropriate interpretation (response rate, 47%). The communication tracking tool was completed by 36 unique residents regarding 151 patients, with a 95% completion rate. Results demonstrated that patients and families preferring LOE were less likely to be present on rounds compared with their counterparts preferring English (adjusted odds ratio, 0.17; 95% confidence interval [CI], 0.07–0.39). Similarly, patients and families preferring LOE were less likely to receive a resident update after rounds (adjusted odds ratio, 0.29; 95% CI, 0.13–0.62) and received fewer resident updates overall (incidence rate ratio, 0.45; 95% CI, 0.30–0.69).

CONCLUSIONS
Hospitalized patients and families preferring LOE experience significant communication-related inequities. Ongoing efforts are needed to promote equitable communication with this population and should consider the unique role of residents.
Six-Year Follow-up of Childhood Stimulation on Development of Children With and Without Anemia
Authors: Hossain Sheikh Jamal , Tofail Fahmida , Mehrin Syeda Fardina , Hamadani Jena D. 
Published: May 2023

BACKGROUND AND OBJECTIVES
Previously, in 30 Bangladeshi villages, 2 groups of children with iron-deficiency anemia (IDA) and nonanemic (NA) iron sufficiency aged 6 to 24 months participated in 2 parallel cluster randomized controlled trials of the effect of psychosocial stimulation on neurodevelopment. The intervention was composed of weekly play sessions at home for 9 months. All children with anemia received iron treatment of 6 months. The intervention improved the mental development of NA but not IDA groups. Six years after end line when the children were aged 8 to 9 years, we aimed to determine if benefits were sustained in the NA group or late-onset benefits emerged in the IDA group.

METHODS
We relocated 372 (90%) of the initial 412 children from all the clusters (villages), and assessed their IQ with the Wechsler Abbreviated Scale of Intelligence-II, motor development, and school achievement including math, spelling, and reading. Analyses were by intention-to-treat, adjusting for clustering.

RESULTS
There was a significant interaction between anemia groups (IDA/NA) and intervention on IQ. The intervention benefitted the NA group’s Full-Scale IQ (effect size, 0.43 [95% confidence interval, 0.08–0.79]) and Perceptual Reasoning Index (effect size, 0.48 [95% confidence interval, 0.08–0.89]) but did not affect the IDA group’s outcomes. No other outcomes were significant.

CONCLUSIONS
The benefits from early childhood psychosocial stimulation on the NA group’s IQ, 6 years after intervention ended, adds to the limited evidence on the sustainability of benefits in low- and middle-income countries. Reasons for lack of effect in children with anemia are unknown.
A Remote Parenting Program and Parent and Staff Perspectives: A Randomized Trial
Authors: Smith Joanne A. , Chang Susan M. , Brentani Alexandra , Fink Günther , Lopez-Boo Florencia , Torino Belen Michel , Codina Marta Rubio , Walker Susan P. 
Published: May 2023

OBJECTIVES
To assess impact and implementation of remote delivery of a parenting program following suspension of in-person visits during the coronavirus disease 2019 pandemic.

METHODS
Impact of remote delivery of the Reach Up parenting program on parenting practices was evaluated by randomized trial in Jamaica. Mothers with children aged 5 to 24 months who met 1 of 7 at-risk criteria were enrolled at health centers. Participants were randomly assigned to intervention or control using random number tables generated by a statistician. Intervention comprised a manual for parents with illustrated play activities, phone calls, and short message service messages. The control group received usual care. Parent practices were measured using an adapted Family Care Indicators telephone-administered questionnaire by interviewers unaware of group assignment. Qualitative interviews were conducted with staff and parents in Jamaica and Brazil and staff in Ecuador to identify facilitators and barriers to remote delivery of Reach Up.

RESULTS
Two hundred forty-seven participants were assessed at endline (control n = 130; intervention n = 117). Intervention increased parent activities that support child development, effect size 0.34 SD (95% confidence interval 0.03–0.53), and use of praise, odds 2 times higher with intervention. There were no benefits to interactive language or play materials. Qualitative results showed parents appreciated program continuation and felt motivated to help their child, and methods were acceptable to staff. Barriers included poor mobile phone access, difficulty contacting parents, and feedback limitations without in-person contact.

CONCLUSIONS
Remote delivery methods have potential to contribute to scaling of parenting programs.
Adapting Reach Up and Learn in Crisis and Conflict Settings: An Exploratory Multiple Case Study
Authors: Wilton Katelin Swing , Murphy Katie Maeve , Mahmud Ahsan , Azam Syful , Habib Anika , Ibrahim Iman , Della Neve Eloisa , Pena Gabriela , Mehrin Syeda Fardina , Shiraji Shamima , Hamadani Jena Derakhshani 
Published: May 2023

OBJECTIVES
In 2019, &gt;71 million children aged &lt;5 had spent their entire lives in conflict-affected settings. Compounding adversities including violence, poverty, and displacement have immediate and long-term effects on early childhood development, health, behavior, and well-being. In response, adaptations of Reach Up have been implemented in conflict and crisis settings.

METHODS
This article uses exploratory multiple case study methodology, drawing from implementation and qualitative data from 3 interventions: a mobile phone-based intervention promoting nurturing care among Rohingya and crisis-affected host communities in Bangladesh; Reach Up amid acute violence and displacement in Northeast Syria; and Reach Up group sessions and home visits integrated with health services for an indigenous population in Venezuela.

RESULTS
In Bangladesh, tailoring interactive voice response messages improved responsiveness to the developmental needs of young children, yet complementary in-person services were identified as a key program enhancement. In Syria, rapid adaptations of Reach Up addressed the needs of families in acute crisis, including social–emotional learning games for school-aged children. In Venezuela, Reach Up, coupled with complementary lactation counseling, yielded high rates of uptake and satisfaction, and children’s language development was highlighted as a key area of growth.

CONCLUSIONS
Recommendations to promote early childhood development in crisis and conflict settings include: (1) cultural adaptation based on a holistic understanding of children and caregivers’ needs; (2) the integration of child and family safety and linkages with complementary services on the basis of community needs and priorities, and (3) the importance of designing for scale through blended models and costing analyses.
Integrated Early Childhood Development in Cambodia: Protocol of a Cluster Stepped-Wedge Trial
Authors: King Katherine Merseth , Yeng Seng , Brennan Claire , Creel Darryl , Ames John W. , Cotes Gwyneth , Bann Carla M. , Black Maureen M. 
Published: May 2023

OBJECTIVES
Limited evidence is available on mechanisms linking integrated, multisector interventions with early childhood development. The Integrated Early Childhood Development program aims to improve children’s development by promoting targeted caregiving behaviors beginning prenatally through age 5 years, in partnership with the Royal Government of Cambodia.

METHODS
This cluster stepped-wedge trial is being conducted in Cambodia among 3 cohorts, encompassing 339 villages and 1790 caregivers who are pregnant or caring for a child aged &lt;5 years. The 12- to 15-month intervention is delivered to each cohort using a staggered stepped-wedge design. Among all cohorts, enrollment evaluations will be followed by 3 data collection waves. Targeted caregiving interventions are provided through community, group, and home-visiting platforms. Child development is measured using the Caregiver Reported Early Development Instrument and the Early Childhood Development Index 2030. The evaluation assesses mediation through targeted caregiving behaviors: responsive caregiving, nutrition, health and hygiene, and household stability and support; moderation by household wealth, caregiver education, and child birth weight; and sustainability after the intervention concludes.

CONCLUSIONS
This protocol article describes the plans for a cluster randomized controlled trial to measure the impact of an integrated, multisector intervention on children’s development. By partnering with the Royal Government of Cambodia and addressing intervention pathways and moderators, this trial will provide guidance for policies and programs to promote early childhood development using principles of implementation science and equity, including increased investment for vulnerable families.
Modelling the effects of climate and land-cover changes on the potential distribution and landscape connectivity of three Earthsnakes (Genus Conopsis, Günther, 1858) in central Mexico.
Authors: Sunny Armando , Manjarrez Javier , Caballero-Viñas Carmen , Bolom-Huet René , Gómez-Ortiz Yuriana , Domínguez-Vega Hublester , Heredia-Bobadilla Rosa Laura , Torres-Romero Erik Joaquín , González-Fernández Andrea 
Published: May 2023

AbstractAnthropogenic changes, such as land use and climate change, are the greatest threats to biodiversity, especially for reptiles, the second most endangered vertebrate class worldwide. Species distribution modelling was applied to assess how expected scenarios of future climate and land cover change might influence the geographic distribution and habitat connectivity of threeConopsisearthsnakes endemic to Mexico:Conopsis biserialis(Taylor and Smith, 1942),Conopsis lineata(Kennicott, 1859), andConopsis nasus(Günther, 1858). In order to do this, two climate models were used, CNRM-CM5 (CN) and MPI-ESM-LR (MP), with the Representative Concentration Pathways of RCP 85 and the Maxent modelling method in ENMeval. The most important environmental variables affecting the distribution of the target species, as well as the environmental niche overlap between them, were also determined.C. biserialis, C. lineata,andC. nasuswere found to be restricted by maximum and minimum temperatures as they are adapted to high elevations and temperate semi-cold climates; therefore, climate was a main determining factor for species distribution. As expected, allConopsisspecies were predicted to face a reduction in their potential distribution withC. lineatapossibly losing approximately 48.5% andC. biserialislosing 28.7%. However, the models were inconsistent in determining which species would suffer the biggest reduction in distribution in the future. Likewise, a reduction in connectivity was found for the three species ofConopsis, whilethe Sierra de las Cruces and Corredor Biológico Chichinautzin were determined as very important areas in terms of connectivity. Unfortunately, great losses ofAbiesandPinusforests occurred in these areas between 2011 and 2014, along with an increase in the agricultural activity that compromises the populations of these species when intensively managed. Most of the potential distribution of these species are found in close proximity to agriculture and outside of protected areas, which is why it is extremely important to create protected areas for reptile species that live in these parts of central Mexico.