bluuebunny/crossref_metadata_2025_split huggingface dataset:
Hippocampal expression of the cannabinoid receptor type 1 in canine epilepsy
Authors: Kostic D. , Nowakowska M. , Freundt Revilla J. , Attig F. , Rohn K. , Gualtieri F. , Baumgärtner W. , Potschka H. , Tipold A. 
Published: February 2023

AbstractCanine drug-resistant epilepsy is a prevailing issue in veterinary neurology. Alternative or additional treatment with cannabinoids is showing promising results in seizure management. A crucial component of the endocannabinoid system, cannabinoid receptor type 1 (CB1R), is heavily involved in the control of neurotransmitter release. Knowledge of its distribution in the epileptic brain would serve a better understanding of disease pathology and application of cannabinoids in dogs with epilepsy. CB1R distribution was assessed in sub-regions of hippocampus of dogs with idiopathic epilepsy, structural epilepsy and without cerebral pathology. In dogs with idiopathic epilepsy, significantly decreased CB1R expression compared to control animals was observed in CA1. In dogs with structural epilepsy, a significant increase in CB1R signal intensity in comparison to controls was observed. CB1R expression was higher in the structural group as compared to the idiopathic. Double immunofluorescence showed co-localization between CB1R and an astrocytic marker in about 50% of cells, regardless of the diagnosis. In summary, CB1R expression in canine hippocampus undergoes modification by the epileptic process and the direction of this change depends on the etiology of the disease. The distinct disease-associated CB1R expression needs to be considered in new treatment development for dogs with epilepsy.
Domes and semi-capsules as model systems for infrared microspectroscopy of biological cells
Authors: Solheim Johanne Heitmann , Brandsrud Maren Anna , Kong Beibei , Banyasz Akos , Borondics Ferenc , Micouin Guillaume , Lossius Stine , Sulé-Suso Josep , Blümel Reinhold , Kohler Achim 
Published: February 2023

AbstractIt is well known that infrared microscopy of micrometer sized samples suffers from strong scattering distortions, attributed to Mie scattering. The state-of-the-art preprocessing technique for modelling and removing Mie scattering features from infrared absorbance spectra of biological samples is built on a meta model for perfect spheres. However, non-spherical cell shapes are the norm rather than the exception, and it is therefore highly relevant to evaluate the validity of this preprocessing technique for deformed spherical systems. Addressing these cases, we investigate both numerically and experimentally the absorbance spectra of 3D-printed individual domes, rows of up to five domes, two domes with varying distance, and semi-capsules of varying lengths as model systems of deformed individual cells and small cell clusters. We find that coupling effects between individual domes are small, corroborating previous related literature results for spheres. Further, we point out and illustrate with examples that, while optical reciprocity guarantees the same extinction efficiency for top vs. bottom illumination, a scatterer’s internal field may be vastly different in these two situations. Finally, we demonstrate that the ME-EMSC model for preprocessing infrared spectra from spherical biological systems is valid also for deformed spherical systems.
Investigating responses to object-labels in the domestic dog (Canis familiaris)
Authors: Kőszegi Hanna , Fugazza Claudia , Magyari Lilla , Iotchev Ivaylo Borislavov , Miklósi Ádám , Andics Attila 
Published: February 2023

AbstractSince the dawn of comparative cognitive research, dogs were suspected to possess some capacity for responding to human spoken language. Neuroimaging studies have supported the existence of relevant mechanisms, but convincing behavioral performance is rare, with only few exceptional dogs worldwide demonstrating a lexicon of object-labels they respond to. In the present study we aimed to investigate if and how a capacity for processing verbal stimuli is expressed in dogs (N = 20), whose alleged knowledge of verbal labels is only backed-up by owner reports taken at face value, and concerning only a few words (on average 5). Dogs were tested in a two-choice paradigm with familiar objects. The experiment was divided into a cue-control condition (objects visible to the owner vs. shielded by a panel, thereby controlling the owner’s ability to emit cues to the dog) and a response type condition (fetching vs. looking). Above chance performance in fetching and looking at the named object emerged on the level of the sample as a whole. Only one individual performed reliably above chance, but the group-level effect did not depend on this data point. The presence of the panel also had no influence, which supports that performance was not driven by non-verbal cues from the owners. The group-level effect suggests that in typical dogs object-label learning is an instable process, either due to the animals primarily engaging in contextual learning or possibly analogous to the early stages of implicit, statistical learning of words in humans and opposed to the rapid mapping reported in exceptional dogs with larger passive vocabulary.
Adult height and health-related quality of life in patients born small for gestational age treated with recombinant growth hormone
Authors: Rodrígez José Manuel Rial , Toda Lourdes Ibañez , López Ignacio Díez , Muñoz Jordi Bosch , Fresno Luis Salamanca , Hernández Eugenio Fernández , de Arriba Muñoz Antonio 
Published: February 2023

AbstractHealth related quality of life (HRQoL) is a relevant result when assessing the course of different pathologies and the efficacy of their treatments. HRQoL has been studied previously on adults born small for gestational age (SGA), both in the general population and in patients who had received recombinant human growth hormone (rhGH) treatment, with disparate results. Our study included 50 adults who had received rhGH treatment for the SGA indication in 4 Spanish hospitals. Data have been gathered retrospectively from their clinical records, current weight and height were measured, and patients have been asked to fill out SF-36 and QoLAGHDA quality of life forms, and the Graffar test to evaluate their socio-economical status. Patient’s adult height was – 1.2 ± 0.9 SD, lower than their target height of 1 ± 0.8 SD, but gaining 1.7 ± 1 SD from the beginning of the treatment. SF-36 test results showed lower scoring on Mental Health domains than on those related to Physical Health. No correlation was found between HRQoL results and final height, rhGH treatment duration or puberty. Correlation was indeed found between QoLAGHDA and several domains of SF-36, but QoLAGHDA detected fewer patients with low HRQoL than SF-36. Thus, it is concluded that SGA patient’s follow-up should include a HRQoL, neuro-cognitive and psychiatric assessment in their transition to adult age. Adult SGA patients without catch up growth have impaired HRQoL, especially in mental health domains.
Motor-effector dependent modulation of sensory-motor processes identified by the multivariate pattern analysis of EEG activity
Authors: Choi Kahyun , Woo Sanghum , Lee Joonyeol 
Published: February 2023

AbstractSensory information received through sensory organs is constantly modulated by numerous non-sensory factors. Recent studies have demonstrated that the state of action can modulate sensory representations in cortical areas. Similarly, sensory information can be modulated by the type of action used to report perception; however, systematic investigation of this issue is scarce. In this study, we examined whether sensorimotor processes represented in electroencephalography (EEG) activities vary depending on the type of effector behavior. Nineteen participants performed motion direction discrimination tasks in which visual inputs were the same, and only the effector behaviors for reporting perceived motion directions were different (smooth pursuit, saccadic eye movement, or button press). We used multivariate pattern analysis to compare the EEG activities for identical sensory inputs under different effector behaviors. The EEG activity patterns for the identical sensory stimulus before any motor action varied across the effector behavior conditions, and the choice of motor effectors modulated the neural direction discrimination differently. We suggest that the motor-effector dependent modulation of EEG direction discrimination might be caused by effector-specific motor planning or preparation signals because it did not have functional relevance to behavioral direction discriminability.
Advances on the early cellular events occurring upon exposure of human macrophages to aluminum oxyhydroxide adjuvant
Authors: Masson J.-D. , Badran G. , Domdom M. A. , Gherardi R. K. , Mograbi B. , Authier F. J. , Crépeaux G. 
Published: February 2023

AbstractAluminum compounds are the most widely used adjuvants in veterinary and human vaccines. Despite almost a century of use and substantial advances made in recent decades about their fate and biological effects, the exact mechanism of their action has been continuously debated, from the initial “depot-theory” to the direct immune system stimulation, and remains elusive. Here we investigated the early in vitro response of primary human PBMCs obtained from healthy individuals to aluminum oxyhydroxide (the most commonly used adjuvant) and a whole vaccine, in terms of internalization, conventional and non-conventional autophagy pathways, inflammation, ROS production, and mitochondrial metabolism. During the first four hours of contact, aluminum oxyhydroxide particles, with or without adsorbed vaccine antigen, (1) were quickly recognized and internalized by immune cells; (2) increased and balanced two cellular clearance mechanisms, i.e. canonical autophagy and LC3-associated phagocytosis; (3) induced an inflammatory response with TNF-α production as an early event; (4) and altered mitochondrial metabolism as assessed by both decreased maximal oxygen consumption and reduced mitochondrial reserve, thus potentially limiting further adaptation to other energetic requests. Further studies should consider a multisystemic approach of the cellular adjuvant mechanism involving interconnections between clearance mechanism, inflammatory response and mitochondrial respiration.
NOTCH2NLC GGC repeats are not expanded in Italian amyotrophic lateral sclerosis patients
Authors: Manini Arianna , Gagliardi Delia , Meneri Megi , Antognozzi Sara , Del Bo Roberto , Comi Giacomo Pietro , Corti Stefania , Ronchi Dario 
Published: February 2023

AbstractRepeat expansions in genes other than C9orf72 and ATXN2 have been recently associated with Amyotrophic Lateral Sclerosis (ALS). Indeed, an abnormal number of GGC repeats in NOTCH2NLC has been recently reported in 0.7% of sporadic ALS patients from mainland China. This finding was not confirmed in an ALS cohort of subjects from Taiwan. As the involvement of expanded NOTCH2NLC alleles in ALS is debated, we addressed this point by evaluating NOTCH2NLC repeat expansions in an Italian cohort of ALS patients. A screening analysis of NOTCH2NLC GGC repeats was performed by repeat-primed polymerase chain reaction (RP-PCR) in a cohort of 385 probable/definite ALS Italian patients. Mean age at onset was 60.5 years (SD 13.7), and 60.9% were males. Sporadic cases were 357 (92.7%), and most patients had a spinal onset (71.8%). None of our patients showed the typical sawtooth tail pattern on RP-PCR, thus excluding abnormal repeat expansion in NOTCH2NLC. Overall, we suggest that NOTCH2NLC expanded alleles might be absent or at least extremely rare in ALS Italian patients. Further investigations in larger cohorts with different ethnic backgrounds are required to support the involvement of NOTCH2NLC in ALS.
High durability and stability of 2D nanofluidic devices for long-term single-molecule sensing
Authors: Thakur Mukeshchand , Cai Nianduo , Zhang Miao , Teng Yunfei , Chernev Andrey , Tripathi Mukesh , Zhao Yanfei , Macha Michal , Elharouni Farida , Lihter Martina , Wen Liping , Kis Andras , Radenovic Aleksandra 
Published: February 2023

AbstractNanopores in two-dimensional (2D) membranes hold immense potential in single-molecule sensing, osmotic power generation, and information storage. Recent advances in 2D nanopores, especially on single-layer MoS2, focus on the scalable growth and manufacturing of nanopore devices. However, there still remains a bottleneck in controlling the nanopore stability in atomically thin membranes. Here, we evaluate the major factors responsible for the instability of the monolayer MoS2 nanopores. We identify chemical oxidation and delamination of monolayers from their underlying substrates as the major reasons for the instability of MoS2 nanopores. Surface modification of the substrate and reducing the oxygen from the measurement solution improves nanopore stability and dramatically increases their shelf-life. Understanding nanopore growth and stability can provide insights into controlling the pore size, shape and can enable long-term measurements with a high signal-to-noise ratio and engineering durable nanopore devices.
Mechanically-tunable bandgap closing in 2D graphene phononic crystals
Authors: Kirchhof Jan N. , Bolotin Kirill I. 
Published: February 2023

AbstractWe present a tunable phononic crystal which can be switched from a mechanically insulating to a mechanically conductive (transmissive) state. Specifically, in our simulations for a phononic lattice under biaxial tension (σxx = σyy = 0.01 N m−1), we find a bandgap for out-of-plane phonons in the range of 48.8–56.4 MHz, which we can close by increasing the degree of tension uniaxiality (σxx/σyy) to 1.7. To manipulate the tension distribution, we design a realistic device of finite size, where σxx/σyy is tuned by applying a gate voltage to a phononic crystal made from suspended graphene. We show that the bandgap closing can be probed via acoustic transmission measurements and that the phononic bandgap persists even after the inclusion of surface contaminants and random tension variations present in realistic devices. The proposed system acts as a transistor for MHz-phonons with an on/off ratio of 105 (100 dB suppression) and is thus a valuable extension for phonon logic applications. In addition, the transition from conductive to isolating can be seen as a mechanical analogue to a metal-insulator transition and allows tunable coupling between mechanical entities (e.g. mechanical qubits).
A “one-size-fits-most” walking recognition method for smartphones, smartwatches, and wearable accelerometers
Authors: Straczkiewicz Marcin , Huang Emily J. , Onnela Jukka-Pekka 
Published: February 2023

AbstractThe ubiquity of personal digital devices offers unprecedented opportunities to study human behavior. Current state-of-the-art methods quantify physical activity using “activity counts,” a measure which overlooks specific types of physical activities. We propose a walking recognition method for sub-second tri-axial accelerometer data, in which activity classification is based on the inherent features of walking: intensity, periodicity, and duration. We validate our method against 20 publicly available, annotated datasets on walking activity data collected at various body locations (thigh, waist, chest, arm, wrist). We demonstrate that our method can estimate walking periods with high sensitivity and specificity: average sensitivity ranged between 0.92 and 0.97 across various body locations, and average specificity for common daily activities was typically above 0.95. We also assess the method’s algorithmic fairness to demographic and anthropometric variables and measurement contexts (body location, environment). Finally, we release our method as open-source software in Python and MATLAB.
Groundwater deeper than 500 m contributes less than 0.1% of global river discharge
Authors: Ferguson Grant , McIntosh Jennifer C. , Jasechko Scott , Kim Ji-Hyun , Famiglietti James S. , McDonnell Jeffrey J. 
Published: February 2023

AbstractGroundwater is one of the largest reservoirs of water on Earth but has relatively small fluxes compared to its volume. This behavior is exaggerated at depths below 500 m, where the majority of groundwater exists and where residence times of millions to even a billion years have been documented. However, the extent of interactions between deep groundwater (>500 m) and the rest of the terrestrial water cycle at a global scale are unclear because of challenges in detecting their contributions to streamflow. Here, we use a chloride mass balance approach to quantify the contribution of deep groundwater to global streamflow. Deep groundwater likely contributes <0.1% to global streamflow and is only weakly and sporadically connected to the rest of the water cycle on geological timescales. Despite this weak connection to streamflow, we found that deep groundwaters are important to the global chloride cycle, providing ~7% of the flux of chloride to the ocean.
Strain-level bacterial typing directly from patient samples using optical DNA mapping
Authors: Nyblom My , Johnning Anna , Frykholm Karolin , Wrande Marie , Müller Vilhelm , Goyal Gaurav , Robertsson Miriam , Dvirnas Albertas , Sewunet Tsegaye , KK Sriram , Ambjörnsson Tobias , Giske Christian G. , Sandegren Linus , Kristiansson Erik , Westerlund Fredrik 
Published: February 2023

AbstractBackgroundIdentification of pathogens is crucial to efficiently treat and prevent bacterial infections. However, existing diagnostic techniques are slow or have a too low resolution for well-informed clinical decisions.MethodsIn this study, we have developed an optical DNA mapping-based method for strain-level bacterial typing and simultaneous plasmid characterisation. For the typing, different taxonomical resolutions were examined and cultivated pureEscherichia coliandKlebsiella pneumoniaesamples were used for parameter optimization. Finally, the method was applied to mixed bacterial samples and uncultured urine samples from patients with urinary tract infections.ResultsWe demonstrate that optical DNA mapping of single DNA molecules can identifyEscherichia coliandKlebsiella pneumoniaeat the strain level directly from patient samples. At a taxonomic resolution corresponding toE. colisequence type 131 andK. pneumoniaeclonal complex 258 forming distinct groups, the average true positive prediction rates are 94% and 89%, respectively. The single-molecule aspect of the method enables us to identify multipleE. colistrains in polymicrobial samples. Furthermore, by targeting plasmid-borne antibiotic resistance genes with Cas9 restriction, we simultaneously identify the strain or subtype and characterize the corresponding plasmids.ConclusionThe optical DNA mapping method is accurate and directly applicable to polymicrobial and clinical samples without cultivation. Hence, it has the potential to rapidly provide comprehensive diagnostics information, thereby optimizing early antibiotic treatment and opening up for future precision medicine management.
A high-sucrose diet causes microbiota composition shift and promotes the susceptibility of mice to Salmonella Typhimurium infection
Authors: Liu Jiaxiu , Liu Huanhuan , Teng Yue , Qin Ningbo , Ren Xiaomeng , Xia Xiaodong 
Published: February 2023

A high-sucrose diet causes microbiota composition shift, and promotes the susceptibility of mice to Salmonella Typhimurium infection.
Deep learning: Applications, architectures, models, tools, and frameworks: A comprehensive survey
Authors: Gheisari Mehdi , Ebrahimzadeh Fereshteh , Rahimi Mohamadtaghi , Moazzamigodarzi Mahdieh , Liu Yang , Dutta Pramanik Pijush Kanti , Heravi Mohammad Ali , Mehbodniya Abolfazl , Ghaderzadeh Mustafa , Feylizadeh Mohammad Reza , Kosari Saeed 
Published: February 2023

AbstractDeep Learning (DL) is a subfield of machine learning that significantly impacts extracting new knowledge. By using DL, the extraction of advanced data representations and knowledge can be made possible. Highly effective DL techniques help to find more hidden knowledge. Deep learning has a promising future due to its great performance and accuracy. We need to understand the fundamentals and the state‐of‐the‐art of DL to leverage it effectively. A survey on DL ways, advantages, drawbacks, architectures, and methods to have a straightforward and clear understanding of it from different views is explained in the paper. Moreover, the existing related methods are compared with each other, and the application of DL is described in some applications, such as medical image analysis, handwriting recognition, and so on.
Hosting capacity enhancement of hybrid AC/DC distribution network based on static and dynamic reconfiguration
Authors: Taghavi Moein , Delkhosh Hamed , Moghaddam Mohsen Parsa , Fini Alireza Sheikhi 
Published: February 2023

AbstractThe hybrid AC/DC distribution networks will have a crucial role in the future of smart grids due to their adaptiveness to a variety of DC loads and energy resources. Thus, it is necessary to assess and enhance the Hosting Capacity (HC) of these hybrid networks for the integration of Renewable Energy Sources (RESs). One way to increase the HC without further expansion in the grid is using the potential of Network Reconfiguration (NR). This paper proposes an HC enhancement model that includes AC/DC distribution NR. Two types of NR are considered, namely static, that is, embedding the NR capabilities at the planning stage, and dynamic, that is, using remotely controlled switches as an Active Network Management (ANM) scheme. Besides, two unique potential sources of hybrid AC/DC distribution networks that could be used for the HC enhancement are included, that is, reactive power control of Voltage Source Converters (VSCs) and the mesh operation capability. The problem is formulated as a mixed‐integer linear optimal power flow that aims to maximize the RESs HC under thermal and voltage constraints. Also, stochastic programming is utilized for managing the RESs uncertainties. The simulation results show the accuracy and efficiency of the proposed formulation from various perspectives.
Evidence for non-thermal X-ray emission from the double Wolf-Rayet colliding-wind binary Apep
Authors: del Palacio S. , García F. , De Becker M. , Altamirano D. , Bosch-Ramon V. , Benaglia P. , Marcote B. , Romero G. E. 
Published: February 2023

Context. Massive colliding-wind binaries (CWBs) can be non-thermal sources. The emission produced in their wind-collision region (WCR) encodes information of both the shock properties and the relativistic electrons accelerated in them. The recently discovered system Apep, a unique massive system hosting two Wolf-Rayet stars, is the most powerful synchrotron radio emitter among the known CWBs. It is an exciting candidate in which to investigate the non-thermal processes associated with stellar wind shocks.
Aims. We intend to break the degeneracy between the relativistic particle population and the magnetic field strength in the WCR of Apep by probing its hard X-ray spectrum, where inverse-Compton (IC) emission is expected to dominate.
Methods. We observed Apep with NuSTAR for 60 ks and combined this with a reanalysis of a deep archival XMM-Newton observation to better constrain the X-ray spectrum. We used a non-thermal emission model to derive physical parameters from the results.
Results. We detect hard X-ray emission consistent with a power-law component from Apep. This is compatible with IC emission produced in the WCR for a magnetic field of ≈105–190 mG, corresponding to a magnetic-to-thermal pressure ratio in the shocks of ≈0.007–0.021, and a fraction of ∼1.5 × 10−4 of the total wind kinetic power being transferred to relativistic electrons.
Conclusions. The non-thermal emission from a CWB is detected for the first time in radio and at high energies. This allows us to derive the most robust constraints so far for the particle acceleration efficiency and magnetic field intensity in a CWB, reducing the typical uncertainty of a few orders of magnitude to just within a factor of a few. This constitutes an important step forward in our characterisation of the physical properties of CWBs.