bluuebunny/crossref_metadata_2025_split huggingface dataset:
Unmet need for family planning and associated factors among currently married women in Hawella Tulla subcity, Hawassa, southern Ethiopia: community-based study
Authors: Assefa Abiyu Ayalew , Selassie Samson G. , Mesele Abebayehu , Kebede Henok Bekele , Fikrie Anteneh , Abera Geleta 
Published: February 2023

Abstract
Background
The unmet need for family planning remains a major public health concern in developing countries, especially in sub-Saharan Africa. Similarly, in Ethiopia, the unmet need for family planning is considerably high. However information regarding associated factors of unmet need of family planning is limited, the study area in particular. Thus, this study was aimed at assessing unmet family planning and associated factors among currently married women in Hawella Tulla Subcity.

Methods
A community based cross-sectional study was employed on 436 currently married women. Both bivariable and multivariable logistic regression model were used and having P-value of < 0.05 was considered as independently associated factors. Strength of association of the variable was described using adjusted odd ratios with their 95% confidence interval.

Result
The overall unmet need for family planning among currently married women was found to be 18.1% (95% CI: 14.5%, 21.8%). Having age of below 18 years at first marriage AOR = 1.95 (95% CI: 1.14, 3.33), woman’s not attained formal education AOR = 2.23 (95% CI: 1.02, 4.84), women whose partner had non-supportive for family planning use AOR = 2.32 (95% CI: 1.35, 3.99) and women without media access AOR = 2.13 (95% CI: 1.19, 3.81) were significantly associated with increasing unmet need for family planning.

Conclusions
Despite the presence of high family planning services coverage in the study area, the magnitude of unmet need for family planning is still reasonably high. Having age of below 18 years at first marriage, woman’s not attained formal education, women whose partner had non-supportive for family planning use and inavailability of media access in the house were found to be associated with high unmet need for family planning. Therefore, efforts are needed to empower women through education, avoiding early marriage and encouraging couple-based family planning interventions. Increasing media access is also advisable intervention.
Exploration of the underlying comorbidity mechanism in psoriasis and periodontitis: a bioinformatics analysis
Authors: Lei Hao , Chen Xin , Wang Ziyang , Xing Zixuan , Du Wenqian , Bai Ruimin , He Ke , Zhang Wen , Wang Yan , Zheng Yan 
Published: February 2023

Abstract
Background
Increasing evidence indicates that psoriasis (PSO) and periodontitis (PD) are likely to occur together, however, the underlying mechanism remains unclear.

Materials and methods
The expression profiles of PSO (lesion vs non-lesion, GSE30999, GSE14905) and PD (affected vs unaffected gingival tissue, GSE16134, GSE10334) were downloaded from the GEO database. First, we investigated the common differentially expressed genes (DEGs) of PSO and PD. Then, GO and KEGG enrichment analysis, protein interaction network (PPI) construction, and hub gene identification analysis were carried out. Finally, GO and KEGG enrichment analysis, miRNA interaction analysis, and transcription factors (TFs) interaction analysis for hub genes were performed.

Results
Eighteen DEGs were identified for further analysis, including 15 up-regulated genes and 3 down-regulated genes. 9 hub genes were then identified via Cytohubba, including IL1B, CXCL1, CXCL8, MMP12, CCL18, SELL, CXCL13, FCGR3B, and SELE. Their functions are mainly enriched in two aspects: neutrophil chemotaxis and migration, chemokine activation and interaction. The enriched signaling pathways includes three categories: host defense, inflammation-related signaling pathways, and disease-related pathways. 9 common miRNAs based on experimental evidence and 10 common TFs were further identified in both PSO and PD.

Conclusion
Our study revealed possible comorbidity mechanisms in PSO and PD from the perspective of bioinformatics tentatively. The data can present new insight for joint prevention and treatment of in PSO and PD, as well as provide data support for further prospective studies.
Changes in body composition of patients undergoing hemodialysis during the coronavirus disease 2019 pandemic: a retrospective longitudinal study
Authors: Fukuzaki Narumi , Suzuki Yuta , Uchida Juri , Nakajima Takuya , Yamamoto Shohei , Imamura Keigo , Yoshikoshi Shun , Harada Manae , Matsuzawa Ryota , Kamiya Kentaro , Matsunaga Atsuhiko 
Published: February 2023

Abstract
Background
The spread of coronavirus disease 2019 (COVID-19) has dramatically altered the lifestyles of many people worldwide. Several studies reported that body weight of young adults increased during the COVID-19 pandemic; however, weight loss has been observed in the elderly population. Therefore, trends in body composition due to the COVID-19 pandemic may vary depending on the characteristics of the population. This study aimed to investigate the changes in body mass index (BMI), muscle mass, and fat mass before and during the COVID-19 pandemic among patients undergoing hemodialysis.

Methods
In this retrospective longitudinal study, we enrolled 115 clinically stable outpatients (mean age: 65.7 ± 11.2 years, 62.6% men) who underwent hemodialysis thrice a week. Baseline data were collected between April 2019 and March 2020, before the declaration of the COVID-19 emergency by the Japanese government. The follow-up measurements were performed between July 2020 and March 2021 during the COVID-19 pandemic. Patient characteristics, laboratory data, and BMI measurements were collected from the medical records. Muscle mass and fat mass were measured using bioelectrical impedance analysis.

Results
BMI and fat mass among the study participants were significantly higher during the COVID-19 pandemic than before the pandemic (p < 0.01), but no significant change in muscle mass was observed. A restricted cubic spline function showed that the increase in BMI appeared to correlate well with fat mass, but not with muscle mass.

Conclusions
BMI and fat mass of patients on hemodialysis significantly increased due to preventive measures against the COVID-19 pandemic in Japan. These findings may provide useful information in making nutritional management decisions for patients undergoing hemodialysis during and after the COVID-19 pandemic.
From good sleep to health and to quality of life – a path analysis of determinants of sleep quality of working adults in Abu Dhabi
Authors: Badri Masood , Alkhaili Mugheer , Aldhaheri Hamad , Yang Guang , Albahar Muna , Alrashdi Asma 
Published: February 2023

Abstract
Background
Sleep quality has significant impacts on many aspects of quality of life. Therefore, identifying the association of sleep quality with that quality of life domains could lead to deeper insights for social policymakers and professionals to enhance their understanding of the lives of Abu Dhabi working adults. This research focuses on sleep quality among working people in Abu Dhabi. The direct and indirect associations of sleep quality with various quality-of-life domains such as income and housing, physical and mental health, sport and activities, eating habits and obesity, work-life balance, online hours, and social connections are investigated and discussed.

Methods
Data were drawn from 36,515 full-time employees in both public, and private sectors, obtained from the third Abu Dhabi Quality of Life (QoL) survey aimed to cover all community members using online platforms. Informed by international research on sleep quality, preliminary investigation using correlation analysis and simple regression identified many well-being variables deemed necessary for inclusion in the path model. Path analysis was then performed.

Results
The final path model produced excellent fit measures. The significant variables directly associated with sleep quality included sleeping hours, social connection, satisfaction with income, satisfaction with residence, subjective physical and mental health, income satisfaction, satisfaction with the surrounding environment, frequency of eating healthy food, work-life balance, and online time. In addition, most variables recorded an indirect association to sleep quality through subjective health.

Conclusions
The importance of multidimensional linkages between many well-being factors influencing sleep quality and subjective health is underscored.
Retrograde venous invasion in renal cell carcinoma: a gross diagnosis ﻿
Authors: Athanazio Daniel Abensur , de Souza Maiara Ferreira , do Amaral Maria Estela Pompeu 
Published: February 2023

AbstractRenal cell carcinoma, notably clear cell renal cell carcinoma in particular, show remarkable predisposition for intravenous growth. Retrograde venous invasion results in intravascular emboli in renal sinus and intrarenal veins, and nodules in renal parenchyma (after extravascular spread). In our experience, we observed this phenomenon in five cases out of 204 renal cell tumors: four in clear cell renal cell carcinomas (4/166 or 2.4%) and 12.5% of those clear cell carcinomas with major renal vein invasion (4/34 or 12.5%). Since it is a recently described phenomenon, easily identifiable a bisected specimen, we believe it would be valuable to show our experience in recognizing and reporting the frequency of this finding in our practice.
Is monkeypox going to be the next pandemic?
Authors: Adesola Ridwan Olamilekan , D’Souza Joanna Nicole , Bello Aisha Bisola , Mukwekwe Varaidzo Joyline 
Published: February 2023

Abstract
Background and aims
In the current global scenario, the monkeypox virus has infected over 3000 individuals from endemic countries like Nigeria, along with non-endemic countries like the UK, Canada, the USA, etc. Based on the current information, it has been observed that monkeypox cases have primarily, though not exclusively, been found among men who have sex with men (MSM) in countries such as the UK. This article discusses the recent outbreak of monkeypox, its causes, and the various approaches to combat monkeypox infections.

Methods
We evaluated the trends of recent outbreaks of monkeypox in different countries and compared them to how the COVID-19 pandemic started.

Results
At present, monkeypox has been reported to spread to over 58 countries via skin-to-skin contact, body fluids, contaminated bed sheets, clothing, or respiratory routes. Smallpox vaccines have been proven to have 85% efficacy against monkeypox. To mitigate this current outbreak, WHO urges people to practice good hygiene and safe sex. The documentation of more cases and further onward spread in the countries in member states are most likely to reoccur, and if not contained, we might experience another global pandemic. Therefore, more research is required to avert this problem.

Conclusion
Monkeypox virus is testing if we have complied with COVID-19 pandemic lessons and elucidates the urgency of research required to understand the monkeypox disease.
A review of bridge scour monitoring techniques and developments in vibration based scour monitoring for bridge foundations
Authors: Kazemian Alan , Yee Tien , Oguzmert Metin , Amirgholy Mahyar , Yang Jidong , Goff Dale 
Published: February 2023

AbstractScour is the gradual erosion of the sediment around a bridge foundation and is one of the leading causes of bridge failure. This erosion is caused by turbulence and sediment transport mechanisms and worsens during high-water flow, such as flooding. A severely scoured bridge is a safety concern for commuters. Monitoring systems are sometimes used to provide indications of the scour extent. Most scour monitoring systems require underwater installation, which is inherently difficult to implement for existing structures. Data obtained from such systems may not necessarily be accurate due to factors such as site temperature fluctuations, or the presence of large debris in the channel causing faulty readings during times of high flooding. Inaccuracy in this data is a problem because it could display erroneous results, leading to a false sense of security. Researchers worldwide are exploring vibration-based techniques to monitor scour to overcome this challenge. These techniques can possibly monitor scour without any underwater installation and may be more efficient than the traditional underwater technologies currently implemented. This review piece aims to present a summary of the several types of scour monitoring techniques traditionally used to monitor scour of bridge structures and the advancement in technology for existing monitoring techniques based on the vibration characteristics of bridges. The importance of monitoring scour progression focused on vibration-based techniques will be discussed as well as providing a fair appraisal of these techniques. This review piece shows evidence through laboratory and field experiments that monitoring a structure based on vibrational changes due to scour is possible, and with the advances in technology over the most recent decade, it is now possible to design cost-effective and accurate scour monitoring systems for future field implemented structural health monitoring projects. This evidence is relevant to future researchers for the implementation of prospective bridge vibration-based systems.
Harmonic oscillator problem in the background of a topologically charged Ellis-Bronnikov–type wormhole(a)
Authors: Ahmed Faizuddin 
Published: February 2023

Abstract
In this paper, we study the quantum dynamics of non-relativistic particles in the background of a topological chagred Ellis-Bronnikov–type wormhole space-time. We derived the radial wave equation and obtain the eigenvalue solution through the confluent Heun equation. Afterwards, we study the harmonic oscillator problem in the same wormhole background and solve the wave equation using the same technique. In both cases, the ground state energy level E
1,l
and the wave function

are presented. Finally, we analyze the results and show that the eigenvalue solutions are influenced by the topological defect parameter α and the wormhole throat radius

and get them modified.
Systematic Review and Meta-Analysis of Randomized Controlled Trials for Scalp Block in Craniotomy
Authors: Duda Taylor , Lannon Melissa , Gandhi Pranjan , Martyniuk Amanda , Farrokhyar Forough , Sharma Sunjay 
Published: February 2023

BACKGROUND:
Scalp block is regional anesthetic injection along nerves innervating the cranium. Scalp blocks for craniotomy may decrease postoperative pain and opioid consumption. Benefits may extend beyond the anesthetic period.

OBJECTIVE:
To analyze evidence for scalp block on postoperative pain and opioid use.

METHODS:
This systematic review and meta-analysis, Prospective Register of Systematic Reviews registration (CRD42022308048), included Ovid Medical Literature Analysis and Retrieval System Online, Embase, and Cochrane Central Register of Controlled Trials inception through February 9, 2022. Only randomized controlled trials were included. We excluded studies not reporting either main outcome. Duplicate reviewers performed study selection, risk of bias assessment, data extraction, and evidence certainty Grading of Recommendations Assessment, Development, and Evaluation appraisal. Main outcomes were postoperative pain by visual analog scale within 72 hours and opioid consumption as morphine milligram equivalent (MME) within 48 hours.

RESULTS:
Screening filtered 955 studies to 23 trials containing 1532 patients. Risk of bias was overall low. Scalp block reduced postoperative pain at 2 through 72 hours, visual analog scale mean differences of 0.79 to 1.40. Opioid requirements were reduced at 24 hours by 16.52 MME and 48 hours by 15.63 MME.

CONCLUSION:
Scalp block reduces postoperative pain at 2 through 48 hours and may reduce pain at 72 hours. Scalp block likely reduces opioid consumption within 24 hours and may reduce opioid consumption to 48 hours. The clinical utility of these differences should be interpreted within the context of modest absolute reductions, overall care optimization, and patient populations. This is the first level 1A evidence to evaluate scalp block efficacy in craniotomy.
Warm temperature-triggered developmental reprogramming requires VIL1-mediated, genome-wide H3K27me3 accumulation in Arabidopsis
Authors: Kim Junghyun , Bordiya Yogendra , Xi Yanpeng , Zhao Bo , Kim Dong-Hwan , Pyo Youngjae , Zong Wei , Ricci William A. , Sung Sibum 
Published: February 2023

ABSTRACT
Changes in ambient temperature immensely affect developmental programs in many species. Plants adapt to high ambient growth temperature in part by vegetative and reproductive developmental reprogramming, known as thermo-morphogenesis. Thermo-morphogenesis is accompanied by massive changes in the transcriptome upon temperature change. Here, we show that transcriptome changes induced by warm ambient temperature require VERNALIZATION INSENSITIVE 3-LIKE 1 (VIL1), a facultative component of the Polycomb repressive complex PRC2, in Arabidopsis. Warm growth temperature elicits genome-wide accumulation of H3K27me3 and VIL1 is necessary for the warm temperature-mediated accumulation of H3K27me3. Consistent with its role as a mediator of thermo-morphogenesis, loss of function of VIL1 results in hypo-responsiveness to warm ambient temperature. Our results show that VIL1 is a major chromatin regulator in responses to high ambient temperature.
The role of Evi/Wntless in exporting Wnt proteins
Authors: Wolf Lucie , Boutros Michael 
Published: February 2023

ABSTRACTIntercellular communication by Wnt proteins governs many essential processes during development, tissue homeostasis and disease in all metazoans. Many context-dependent effects are initiated in the Wnt-producing cells and depend on the export of lipidated Wnt proteins. Although much focus has been on understanding intracellular Wnt signal transduction, the cellular machinery responsible for Wnt secretion became better understood only recently. After lipid modification by the acyl-transferase Porcupine, Wnt proteins bind their dedicated cargo protein Evi/Wntless for transport and secretion. Evi/Wntless and Porcupine are conserved transmembrane proteins, and their 3D structures were recently determined. In this Review, we summarise studies and structural data highlighting how Wnts are transported from the ER to the plasma membrane, and the role of SNX3-retromer during the recycling of its cargo receptor Evi/Wntless. We also describe the regulation of Wnt export through a post-translational mechanism and review the importance of Wnt secretion for organ development and cancer, and as a future biomarker.
A CRISPR-del-based pipeline for complete gene knockout in human diploid cells
Authors: Komori Takuma , Hata Shoji , Mabuchi Akira , Genova Mariya , Harada Tomoki , Fukuyama Masamitsu , Chinen Takumi , Kitagawa Daiju 
Published: February 2023

ABSTRACT
The advance of CRISPR/Cas9 technology has enabled us easily to generate gene knockout cell lines by introducing insertion–deletion mutations (indels) at the target site via the error-prone non-homologous end joining repair system. Frameshift-promoting indels can disrupt gene functions by generation of a premature stop codon. However, there is growing evidence that targeted genes are not always knocked out by the indel-based gene disruption. Here, we established a pipeline of CRISPR-del, which induces a large chromosomal deletion by cutting two different target sites, to perform ‘complete’ gene knockout efficiently in human diploid cells. Quantitative analyses show that the frequency of gene deletion with this approach is much higher than that of conventional CRISPR-del methods. The lengths of the deleted genomic regions demonstrated in this study are longer than those of 95% of the human protein-coding genes. Furthermore, the pipeline enabled the generation of a model cell line having a bi-allelic cancer-associated chromosomal deletion. Overall, these data lead us to propose that the CRISPR-del pipeline is an efficient and practical approach for producing ‘complete’ gene knockout cell lines in human diploid cells.
DRP1 mutations associated with EMPF1 encephalopathy alter mitochondrial membrane potential and metabolic programs
Authors: Robertson Gabriella L. , Riffle Stellan , Patel Mira , Bodnya Caroline , Marshall Andrea , Beasley Heather K. , Garza-Lopez Edgar , Shao Jianqiang , Vue Zer , Hinton Antentor , Stoll Maria S. , de Wet Sholto , Theart Rensu P. , Chakrabarty Ram Prosad , Loos Ben , Chandel Navdeep S. , Mears Jason A. , Gama Vivian 
Published: February 2023

ABSTRACT
Mitochondria and peroxisomes are dynamic signaling organelles that constantly undergo fission, driven by the large GTPase dynamin-related protein 1 (DRP1; encoded by DNM1L). Patients with de novo heterozygous missense mutations in DNM1L present with encephalopathy due to defective mitochondrial and peroxisomal fission (EMPF1) – a devastating neurodevelopmental disease with no effective treatment. To interrogate the mechanisms by which DRP1 mutations cause cellular dysfunction, we used human-derived fibroblasts from patients who present with EMPF1. In addition to elongated mitochondrial morphology and lack of fission, patient cells display lower coupling efficiency, increased proton leak and upregulation of glycolysis. Mitochondrial hyperfusion also results in aberrant cristae structure and hyperpolarized mitochondrial membrane potential. Peroxisomes show a severely elongated morphology in patient cells, which is associated with reduced respiration when cells are reliant on fatty acid oxidation. Metabolomic analyses revealed impaired methionine cycle and synthesis of pyrimidine nucleotides. Our study provides insight into the role of mitochondrial dynamics in cristae maintenance and the metabolic capacity of the cell, as well as the disease mechanism underlying EMPF1.
Vps9d1 regulates tubular endosome formation through specific activation of Rab22A
Authors: Nakashima Shumpei , Matsui Takahide , Fukuda Mitsunori 
Published: February 2023

ABSTRACT
The small GTPase Rab22A is an important regulator of the formation of tubular endosomes, which are one of the types of recycling endosome compartments of the clathrin-independent endocytosis pathway. In order to regulate tubular endosome formation, Rab22A must be activated by a specific guanine-nucleotide-exchange factor (GEF); however, all of the GEFs that have been reported to exhibit Rab22A-GEF activity in vitro also activate Rab5A, an essential regulator of the clathrin-mediated endocytosis pathway, and no Rab22A-specific GEF has ever been identified. Here, we identified Vps9d1, a previously uncharacterized vacuolar protein sorting 9 (VPS9) domain-containing protein, as a novel Rab22A-GEF. The formation of tubular endosome structures was found to be severely impaired in Vps9d1-depleted HeLa cells, but Rab5A localization was unaffected. Expression of a constitutively active Rab22A mutant in Vps9d1-depleted HeLa cells restored tubular endosomes, but expression of a GEF-activity-deficient Vps9d1 mutant did not. Moreover, Vps9d1 depletion altered the distribution of clathrin-independent endocytosed cargos and impaired their recycling. Our findings indicate that Vps9d1 promotes tubular endosome formation by specifically activating Rab22A.