bluuebunny/crossref_metadata_2025_split huggingface dataset:
Effect of microencapsulated inoculum of Pichia kudriavzevii on the fermentation and sensory quality of cacao CCN51 genotype
Authors: Criollo Nuñez Jenifer , Ramirez‐Toro Cristina , Bolivar German , Sandoval A. Angélica Piedad , Lozano Tovar María D. 
Published: January 2023

AbstractBACKGROUNDMicroencapsulated yeasts are a novel alternative as a delivery matrix for microbiological starters. This technology aims to protect the active compounds from adverse environmental conditions and prolong their useful life and could also improve the conditions of the starters for cocoa fermentation. The present study established the effective dose to apply the microencapsulated yeast Pichia kudriavzevii as a microbiological starter of fermentation and biotechnological strategy for promoting the biochemical dynamics and sensory expression of the cocoa variety CCN‐51. For this, 0.5%, 1%, 2%, and 3% of microencapsulated P. kudriavzevii yeast insolated from the artisanal fermentation process of cocoa was added to the cocoa mass to be fermented and studied on a laboratory scale.RESULTSThe partial least squares regression of fermentation was related in four quartiles, comprising the hedonic judgments of the sensory evaluation with the biochemical traits of the cocoa liquor, finding a high correlation between the physicochemical variables total phenols, percentage of insufficiently fermented grains, and percentage of total acidity, with a level of bitterness and defects found in liquors with the addition of 0.5% of microencapsulated starter. The treatments with the addition of 2% and 3% of the inoculum showed a high correlation between the variables pH, total anthocyanins, cocoa, fruity and floral aromas, sweet taste, and general aroma perception.CONCLUSIONThe higher presence of volatile compounds such as 2,3‐butanediol associated with cocoa aroma and 1‐phenyl‐2‐ethanol and acetophenone associated with aromatic descriptors of fruity and floral series allowed establishment in 2% of microencapsulated P. kudriavzevii yeast, comprising the effective dose for promoting the biochemical dynamics of laboratory‐scale fermentation and the development of cocoa, as well as the fruity and floral aromas of cocoa CCN‐51 liquor. The microencapsulation is suitable for cocoa starters. © 2023 Society of Chemical Industry.
Rethinking strategic sourcing during disruptions: A resilience‐driven process contribution to knowledge on supply chains
Authors: Frederico Guilherme Francisco 
Published: January 2023

AbstractThis paper aims to present a structured perspective regarding the strategic sourcing process with the aim of enhancing supply chain resilience during disturbing events. For this purpose, it uses a descriptive approach, based on theoretical insights in order to provide a detailed strategic sourcing process, which adopts a risk‐based orientation. Each of the five main steps of the strategic sourcing process that are identified have aspects related to the supply chain resilience that need to be taken into consideration on the sourcing decision‐making. The framework that is presented provides relevant insights regarding on how to effectively conduct the strategic sourcing process with the prescription that decision‐making needs to be transparent. It is hoped that the constructs presented in this paper encourage researchers to conducting empirical studies in order to obtain evidence related to the strategic sourcing process and its relations with supply chain resilience. The proposed framework of this paper, can also potentially support practitioners involved in the procurement and strategic sourcing activities by providing an effective guidance as to how to appropriately engage in decision‐making with respect to strategic sourcing. The approach proposed is unique, bringing a timely and novel approach by relating strategic sourcing with supply chain resilience during disturbing events.
Controlling the Modal Confinement in Silicon Nanophotonic Waveguides through Dual‐Metamaterial Engineering
Authors: Dinh Thi Thuy Duong , Le Roux Xavier , Zhang Jianhao , Montesinos‐Ballester Miguel , Lafforgue Christian , Benedikovic Daniel , Cheben Pavel , Cassan Eric , Marris‐Morini Delphine , Vivien Laurent , Alonso‐Ramos Carlos 
Published: January 2023

AbstractFlexible control of the modal confinement in silicon photonic waveguides is an appealing feature for many applications, including sensing and hybrid integration of active materials. In most cases, strip waveguides are the preferred solution to maximize the light interaction with the waveguide surroundings. However, the only two degrees of freedom in Si strip waveguides are the width and thickness, resulting in limited flexibility in evanescent field control. Here, a new strategy that exploits metamaterial engineering of the waveguide core and cladding is proposed and demonstrated to control the index contrast in the vertical and horizontal directions, independently. The proposed dual‐material geometry yields a substantially increased calculated bulk sensitivity in the air (0.35 RIU [refractive index unit]/RIU) compared to the best case scenario for a strip waveguide (0.3 RIU/RIU). To experimentally demonstrate the potential of this approach, dual‐metamaterial ring resonators operating with the transverse‐magnetic polarized mode in 220‐nm‐thick waveguides with air as upper‐cladding are implemented. Micro‐ring resonators implemented with strip and dual‐metamaterial waveguides exhibit the same measured quality factors, near 30 000. Having similar measured quality factors and better calculated bulk sensitivity than strip waveguides, the proposed dual‐metamaterial geometry stands as a promising approach to control modal confinement in silicon waveguides.
Molecular Simulation and Experimental Study on the Damping and Aging Properties of 4010NA/Hydrogenated Nitrile Butadiene/Nitrile Butadiene Rubber Composites
Authors: Song Meng , Wang Meng , Wang Chaole , Song Jihong , Li Yunan , Cao Fengyi , Yu Guomin , Qin Qi 
Published: January 2023

AbstractThe effects of N‐isopropyl‐N′‐phenyl‐phenylenediamine (4010NA) content on the damping and aging properties of hydrogenated nitrile butadiene rubber (HNBR)/nitrile butadiene rubber (NBR) (abbreviated as H‐NBR) matrix are studied via molecular simulation and experiments. The effects of 4010NA addition on the damping and aging properties of H‐NBR are analyzed by molecular simulation using solubility parameters (δ), hydrogen bonds, free volume fraction (FFV), binding energy (Ebinding), hydrogen bond dissociation energy (ΔG), and mean square displacement (MSD). The damping, mechanical, and thermo‐oxygen aging properties of the 4010NA/H‐NBR composites are studied experimentally using infrared (FTIR) spectroscopy, X‐ray diffraction (XRD), differential scanning calorimetry (DSC), and dynamic mechanical analysis (DMA). The results indicate that 4010NA has good compatibility with HNBR and NBR, and the addition of 4010NA can effectively improve the damping properties of H‐NBR. When 4010NA is added at 32 phr, the composite has better damping properties, mechanical properties, and aging resistance, which provides a new insight for the construction of high performance elastomer composites.
Creutzfeldt–Jakob Disease Misdiagnosed as Multiple System Atrophy
Authors: Martin Nicholas B. , Koga Shunsuke , Appleby Brian S. , Sekiya Hiroaki , Dickson DennisW. 
Published: January 2023

AbstractBackgroundMultiple system atrophy (MSA) is a progressive neurodegenerative disorder characterized by various combinations of autonomic failure, parkinsonism, and cerebellar syndromes. Although consensus criteria have been widely used to diagnose MSA, accurate clinical diagnosis remains challenging. Other neurodegenerative disorders, such as Lewy body disease, can mimic MSA.ObjectivesWe described clinical and neuropathologic findings of two patients with Creutzfeldt–Jakob disease (CJD) who had antemortem clinical diagnoses of MSA.MethodsThe brain bank for neurodegenerative disorders was queried for cases with a clinical diagnosis of MSA, but neuropathologic findings of CJD.ResultsCase 1 was a 55‐year‐old man with a 6‐month history of orthostatic hypotension, parkinsonism, cerebellar ataxia, bradyphrenia, and memory impairment. Case 2 was a 65‐year‐old man who had a 5‐year history of cerebellar ataxia, parkinsonism, and cognitive impairment, as well as a 7‐year history of dream enactment behavior. Neither case had characteristic α‐synuclein immunoreactive neuronal or glial inclusions typical of MSA. Instead, they had spongiform encephalopathy with neuronal loss and gliosis with prion protein‐immunoreactive kuru‐like plaques. Genetic analyses in case 1 had wild‐type PRNP, whereas case 2 revealed a 4‐octapeptide repeat insertion in PRNP.ConclusionsEven when clinical features suggest MSA, CJD should also be considered if the progression is rapid or the disease course is atypical, such as the absence of autonomic dysfunction for an extended period.
Bi‐allelic loss of function variant in the NRCAM gene is associated with motor‐predominant axonal polyneuropathy; the second report
Authors: Elahi Zohreh , Soveyzi Mohamad , Nafissi Shahriar , Nilipour Yalda , Goleyjani Moghadam Masoumeh , Keshavarz Elham , Kariminejad Ariana , Najmabadi Hossein , Fattahi Zohreh 
Published: January 2023

AbstractBackgroundThe role of biallelic variants in the NRCAM gene underlying a neurodevelopmental disorder has been defined recently. The phenotype is mainly recognized by varying severity of global developmental delay/intellectual disability, hypotonia, spasticity, and peripheral neuropathy.MethodsHere, we describe a patient with an initial diagnosis of motor‐predominant axonal polyneuropathy or a form of distal SMA. Whole‐exome sequencing (WES), in parallel with WES‐based CNV detection and assessment of homozygosity runs, was performed to identify this patient's possible genetic cause.ResultsWhole exome sequencing revealed a homozygous variant, c.73C > T (p.Gln25*), in the NRCAM gene, while the patient manifests a mild range of phenotypes compared to NRCAM‐related disorder. He presented only motor‐predominant axonal polyneuropathy with no other signs of central nervous system involvement.ConclusionsThis study is the second report of an association between biallelic NRCAM gene variants and a Mendelian disorder. The obtained clinical data, together with the molecular findings in this patient, expands the clinical and molecular spectrum of NRCAM‐related disorder and highlights its phenotypic complexity. Although patients with loss of function variants in this gene have previously presented severe clinical features, we show that type of the pathogenic variant does not necessarily determine the severity of this phenotype.
Defective E2 electrode lead gives low‐amplitude compound muscle action potential
Authors: Nandedkar Sanjeev D. , Barkhaus Paul E. 
Published: January 2023

AbstractIntroduction/AimsLow‐amplitude compound muscle action potential (CMAP) suggests a neuromuscular pathology. Low amplitude will also result from a defective E1 electrode or its lead, that is, a technical artifact. The aim of this study was to investigate the effect of a defective E2 electrode lead on the CMAP.MethodsThe CMAP was recorded using standard nerve conduction methodology and all electrode leads connected properly. Signals were then recorded when either the E1 or the E2 electrode lead was disconnected from the amplifier. This simulated a defective electrode lead. Studies were performed in four nerves of a healthy subject.ResultsCMAP amplitude was reduced as expected when E1 was disconnected. Surprisingly, the amplitude fell by more than 65% when the E2 lead was disconnected, although E1 was properly connected.DiscussionE1 and E2 electrodes contribute to the CMAP. A defective recording electrode lead to E1 or E2 results in a low‐amplitude CMAP. The amplitude drop observed with a disconnected E2 lead was far greater than the signal recorded by the E2 electrode. This occurs due to the amplifier's inherent property to reduce the voltage difference between the E1 and E2 inputs. When E2 lead is defective, the CMAP will be an attenuated version of the signal recorded by the E1 electrode, and vice versa. When low‐amplitude CMAP amplitude is observed in all conduction studies, technical artifact should be considered before exploring the pathological basis for the abnormal results.
Modeling the Population Response of Alligator Gar in Texoma Reservoir to Harvest and Discard Mortality
Authors: Snow Richard S. , Stewart David R. , Smith Nathan G. , Porta Michael J. 
Published: January 2023

Abstract
The popularity of angling for Alligator Gar Atractosteus spatula is an essential component of fisheries management and can affect population resilience. In most cases with Alligator Gar, development of biological assessment models to assess stocks is often unavailable. For this reason, we aimed at describing Alligator Gar demographic rates and population size in Texoma Reservoir, Oklahoma. We used these data to parameterize an age-structured simulation model to determine population responses of maximized yield, total catch, catch of trophy fish, and fishery sustainability to a range of instantaneous finite fishing mortality rates (FM) and discard mortality rates (D) in approximating ideal harvest strategies. Our simulations suggest that the Alligator Gar population is highly sensitive to increases in exploitation. Under the current management regulations, an FM of 0.00–0.05 would have a limited impact on sustainability, which would result in negligible growth as an effect of stochastic recruitment. If FM reaches or exceeds 0.06, the current regulations would not be effective at sustaining the population (spawning potential ratio &lt;0.30). At FM values greater than 0.10, the probability of the population declining from the current population estimate (1,947 fish) to less than 500 individuals would increase from 20% to 100%, resulting in the collapse of the population over 100 years, especially if D is greater than 0.10. Use of a 1,524-mm minimum length limit had the potential to allow FM values of up to 0.10 while absorbing a low amount of D. The use of length limits may provide some options for sustaining the Alligator Gar population, although their efficacy is limited by D when FM exceeds 0.06. Given the sensitivity of long-lived, periodically recruiting fishes to exploitation and given the benefits that possible length-based regulations or a tag/lottery system can provide, we encourage managers to continue monitoring Alligator Gar populations, and when concerning trends are observed, managers should consider such regulations to increase resilience to FM and increase sustainability.
Analysis of the effect of brexpiprazole on sleep architecture in patients with schizophrenia: A preliminary study
Authors: Arai Yusuke , Sasayama Daimei , Kuraishi Kazuaki , Murata Shiho , Usuda Nobuteru , Tsuchida Mika , Nakajima Yuka , Washizuka Shinsuke 
Published: January 2023

AbstractBackgroundBrexpiprazole is an atypical antipsychotic drug widely used in Japan for the treatment of schizophrenia. Previous studies have investigated the therapeutic effects of some antipsychotics on sleep variables; however, to our knowledge, the effects of brexpiprazole on sleep architecture have not been examined in patients with schizophrenia. Therefore, we aimed to exploratorily investigate the effect of brexpiprazole on sleep variables measured by polysomnography in patients with schizophrenia.MethodsThis study included 10 patients with schizophrenia who were originally treated with haloperidol alone. Sleep variables of the participants were measured using polysomnography. After excluding those who did not meet the study criteria, seven patients (five men and two women; mean age [SD], 59.0 [10.0] years) were eligible for further analysis. Polysomnography was repeated at 4 weeks after the participants were prescribed brexpiprazole in addition to haloperidol. We compared the sleep architecture of the participants, measured using polysomnography, before and after taking brexpiprazole.ResultsAdd‐on brexpiprazole significantly prolonged rapid eye movement latency, increased the duration and percentage of stage N2 and stage N3 sleep (min, %), and decreased the duration and percentage of stage rapid eye movement sleep (min, %) at a significance level of nominal p < 0.05.ConclusionAlthough not significant after correcting for multiple comparisons, the present results showed that add‐on brexpiprazole could alter the sleep architecture of patients with schizophrenia. Future studies are warranted to replicate these findings and to further investigate the beneficial influence of brexpiprazole on sleep.
Precision fluid and vasoactive drug therapy for critically ill patients
Authors: Bauer Seth R. , Gellatly Rochelle M. , Erstad Brian L. 
Published: January 2023

AbstractThere are several clinical practice guidelines concerning the use of fluid and vasoactive drug therapies in critically ill adult patients, but the recommendations in these guidelines are often based on low‐quality evidence. Further, some were compiled prior to the publication of landmark clinical trials, particularly in the comparison of balanced crystalloid and normal saline. An important consideration in the treatment of critically ill patients is the application of precision medicine to provide the most effective care to groups of patients most likely to benefit from the therapy. Although not currently widely integrated into these practice guidelines, the utility of precision medicine in critical illness is a recognized research priority for fluid and vasoactive therapy management. The purpose of this narrative review was to illustrate the evaluation and challenges of providing precision fluid and vasoactive therapies to adult critically ill patients. The review includes a discussion of important investigations published after the release of currently available clinical practice guidelines to provide insight into how recommendations and research priorities may change future guidelines and bedside care for critically ill patients.
Metabolic Profiling of Murine Macrophages Exposed to Silver Nanoparticles at Dose and Time Dependencies
Authors: Palomares Cristofher Victor Vivas , Barreto Yan Borges , Bexiga Natalia Marchesan , Toma Sergio Hiroshi , Julival dos Santos Jonnatan , Araki Koiti , Alencar Adriano Mesquita , Bloise Antonio Carlos 
Published: January 2023

AbstractThe macrophage time‐dependent metabolic profile changing basal metabolism triggered by nanoparticles can be obtained and used to improve wound healing treatments. Herein this study demonstrates that metabolic status responds systematically to cytotoxicity manipulation, providing an interesting way of cellular control. Nuclear magnetic resonance (NMR) based metabolomics and cytotoxic assays are used to study RAW 264.7 cells exposed to AgNPs at different concentrations and incubation times. Cytotoxicity data show a slight decrease in cellular expansion rates accompanied by morphological changes in cells. Metabolomics show that despite the glycolytic activity of treated and non‐treated cells remains unchanged; however, only the treated cells present a rich Citrate environment signaling up‐regulation of Tricarboxylic‐Acid‐Cycle (TCA). Cells choose aerobic routes instead of anaerobic ones to produce energy and self‐regulate their amino acid metabolism to balance TCA. Choline metabolism is down‐regulated once its sub‐products, Betaine and Glycine, are reduced, thus compromising Creatine synthesis. Phospholipid metabolism is down‐regulated due to the decreasing of Phosphocholine and Sn‐Glycerol‐3‐PC, in agreement with the cytotoxicity results. Pyroglutamate decreases in treated cells, signaling different levels of oxidative stress. These analytical tools can characterize AgNPs‐treatments, even distinguishing dose and time dependencies. Therefore, the fine‐tuning of exposition parameters can modulate cellular activity to achieve better wound healing.
The human protein atlas—Integrated omics for single cell mapping of the human proteome
Authors: Digre Andreas , Lindskog Cecilia 
Published: January 2023

AbstractStudying the spatial distribution of proteins provides the basis for understanding the biology, molecular repertoire, and architecture of every human cell. The Human Protein Atlas (HPA) has grown into one of the world's largest biological databases, and in the most recent version, a major update of the structure of the database was performed. The data has now been organized into 10 different comprehensive sections, each summarizing different aspects of the human proteome and the protein‐coding genes. In particular, large datasets with information on the single cell type level have been integrated, refining the tissue and cell type specificity and detailing the expression in cell states with an increased resolution. The multi‐modal data constitute an important resource for both basic and translational science, and hold promise for integration with novel emerging technologies at the protein and RNA level.
Solution X‐ray scattering highlights discrepancies in Plasmodiummulti‐aminoacyl‐tRNA synthetase complexes
Authors: Jaramillo Ponce José R. , Théobald‐Dietrich Anne , Bénas Philippe , Paulus Caroline , Sauter Claude , Frugier Magali 
Published: January 2023

AbstracttRip is a tRNA import protein specific to Plasmodium, the causative agent of malaria. In addition to its membrane localization and tRNA trafficking properties, tRip has the capacity to associate with three aminoacyl‐tRNA synthetases (aaRS), the glutamyl‐ (ERS), glutaminyl‐ (QRS), and methionyl‐ (MRS) tRNA synthetases. In eukaryotes, such multi‐aaRSs complexes (MSC) regulate the moonlighting activities of aaRSs. In Plasmodium, tRip and the three aaRSs all contain an N‐terminal GST‐like domain involved in the assembly of two independent complexes: the Q‐complex (tRip:ERS:QRS) and the M‐complex (tRip:ERS:MRS) with a 2:2:2 stoichiometry and in which the association of the GST‐like domains of tRip and ERS (tRip‐N:ERS‐N) is central. In this study, the crystal structure of the N‐terminal GST‐like domain of ERS was solved and made possible further investigation of the solution architecture of the Q‐ and M‐complexes by small‐angle x‐ray scattering (SAXS). This strategy relied on the engineering of a tRip‐N‐ERS‐N chimeric protein to study the structural scaffold of both Plasmodium MSCs and confirm the unique homodimerization pattern of tRip in solution. The biological impact of these structural arrangements is discussed.