bluuebunny/crossref_metadata_2025_split huggingface dataset:
Identification and Characterization of the Wilms Tumor Cancer Stem Cell
Authors: Petrosyan Astgik , Villani Valentina , Aguiari Paola , Thornton Matthew E. , Wang Yizhou , Rajewski Alex , Zhou Shengmei , Cravedi Paolo , Grubbs Brendan H. , De Filippo Roger E. , Sedrakyan Sargis , Lemley Kevin V. , Csete Marie , Da Sacco Stefano , Perin Laura 
Published: April 2023

AbstractA nephrogenic progenitor cell (NP) with cancer stem cell characteristics driving Wilms tumor (WT) using spatial transcriptomics, bulk and single cell RNA sequencing, and complementary in vitro and transplantation experiments is identified and characterized. NP from WT samples with NP from the developing human kidney is compared. Cells expressing SIX2 and CITED1 fulfill cancer stem cell criteria by reliably recapitulating WT in transplantation studies. It is shown that self‐renewal versus differentiation in SIX2+CITED1+ cells is regulated by the interplay between integrins ITGβ1 and ITGβ4. The spatial transcriptomic analysis defines gene expression maps of SIX2+CITED1+ cells in WT samples and identifies the interactive gene networks involved in WT development. These studies define SIX2+CITED1+ cells as the nephrogenic‐like cancer stem cells of WT and points to the renal developmental transcriptome changes as a possible driver in regulating WT formation and progression.
Multiphysical Field Modulated VO2 Device for Information Encryption
Authors: Zhao Shanguang , Li Liang , Hu Changlong , Li Bowen , Liu Meiling , Zhu Jinglin , Zhou Ting , Shi Weidong , Zou Chongwen 
Published: April 2023

AbstractIn the information explosion society, information security is highly demanded in the practical application, which raised a surge of interest in designing secure and reliable information transmission channels based on the inherent properties of emerging devices. Here, an innovative strategy to achieve the data encryption and reading during the data confidential transmission based on VO2 device is proposed. Owing to the specific insulator‐to‐metal transition property of VO2, the phase transitions between the insulator and metallic states are modulated by the combination of electric field, temperature, and light radiation. These external stimulus‐induced phase diagram is directly associated with the defined VO2 device, which are applicable for control the “0” or “1” electrical logic state for the information encryption. A prototype device is fabricated on an epitaxial VO2 film, which displayed a unique data encryption function with excellent stability. The current study not only demonstrated a multiphysical field‐modulated VO2 device for information encryption, but also supplied some clues for functional devices applications in other correlated oxide materials.
Accurate and Rapid Detection of Peritoneal Metastasis from Gastric Cancer by AI‐Assisted Stimulated Raman Molecular Cytology
Authors: Chen Xun , Wu Zhouqiao , He Yexuan , Hao Zhe , Wang Qi , Zhou Keji , Zhou Wanhui , Wang Pu , Shan Fei , Li Zhongwu , Ji Jiafu , Fan Yubo , Li Ziyu , Yue Shuhua 
Published: April 2023

AbstractPeritoneal metastasis (PM) is the mostcommon form of distant metastasis and one of the leading causes of death in gastriccancer (GC). For locally advanced GC, clinical guidelines recommend peritoneal lavage cytology for intraoperative PM detection. Unfortunately, current peritoneal lavage cytology is limited by low sensitivity (<60%). Here the authors established the stimulated Raman molecular cytology (SRMC), a chemical microscopy‐based intelligent cytology. The authors firstly imaged 53 951 exfoliated cells in ascites obtained from 80 GC patients (27 PM positive, 53 PM negative). Then, the authors revealed 12 single cell features of morphology and composition that are significantly different between PM positive and negative specimens, including cellular area, lipid protein ratio, etc. Importantly, the authors developed a single cell phenotyping algorithm to further transform the above raw features to feature matrix. Such matrix is crucial to identify the significant marker cell cluster, the divergence of which is finally used to differentiate the PM positive and negative. Compared with histopathology, the gold standard of PM detection, their SRMC method could reach 81.5% sensitivity, 84.9% specificity, and the AUC of 0.85, within 20 minutes for each patient. Together, their SRMC method shows great potential for accurate and rapid detection of PM from GC.
Higher systolic blood pressure in early‐mid adulthood is associated with poorer cognitive performance in those with a dominantly inherited Alzheimer's disease mutation but not in non‐carriers. Results from the DIAN study
Authors: Xu Ying , Aung Htein Linn , Bateman Randall J. , Brooks William S. , Chhatwal Jasmeer , Day Gregory S. , Fagan Anne M. , Farlow Martin R. , Gordon Brian , Kehoe Patrick G. , Levin Johannes , Mori Hiroshi , Morris John C. , Wharton Whitney , Humburg Peter , Schofield Peter R. , Peters Ruth ,   Dominantly Inherited Alzheimer Network (DIAN)
Published: April 2023

AbstractBACKGROUNDThe Dominantly Inherited Alzheimer Network (DIAN) is a longitudinal observational study that collects data on cognition, blood pressure (BP), and other variables from autosomal‐dominant Alzheimer's disease mutation carriers (MCs) and non‐carrier (NC) family members in early to mid‐adulthood, providing a unique opportunity to evaluate BP and cognition relationships in these populations.METHODWe examined cross‐sectional and longitudinal relationships between systolic and diastolic BP and cognition in DIAN MC and NC.RESULTSData were available from 528 participants, who had a mean age of 38 (SD = 11) and were 42% male and 61% MCs, at a median follow‐up of 2 years. Linear‐multilevel models found only cross‐sectional associations in the MC group between higher systolic BP and poorer performance on language (β = −0.181 [−0.318, −0.044]), episodic memory (−0.212 [−0.375, −0.049]), and a composite cognitive measure (−0.146 [−0.276, −0.015]). In NCs, the relationship was cross‐sectional only and present for language alone.DISCUSSIONHigher systolic BP was cross‐sectionally but not longitudinally associated with poorer cognition, particularly in MCs. BP may influence cognition gradually, but further longitudinal research is needed.
Comparing machine learning‐derived MRI‐based and blood‐based neurodegeneration biomarkers in predicting syndromal conversion in early AD
Authors: Cai Yuan , Fan Xiang , Zhao Lei , Liu Wanting , Luo Yishan , Lau Alexander Yuk Lun , Au Lisa Wing Chi , Shi Lin , Lam Bonnie Y. K. , Ko Ho , Mok Vincent Chung Tong 
Published: April 2023

AbstractIntroductionWe compared the machine learning‐derived, MRI‐based Alzheimer's disease (AD) resemblance atrophy index (AD‐RAI) with plasma neurofilament light chain (NfL) level in predicting conversion of early AD among cognitively unimpaired (CU) and mild cognitive impairment (MCI) subjects.MethodsWe recruited participants from the Alzheimer's Disease Neuroimaging Initiative (ADNI) who had the following data: clinical features (age, gender, education, Montreal Cognitive Assessment [MoCA]), structural MRI, plasma biomarkers (p‐tau181, NfL), cerebrospinal fluid biomarkers (CSF) (Aβ42, p‐tau181), and apolipoprotein E (APOE) ε4 genotype. We defined AD using CSF Aβ42 (A+) and p‐tau181 (T+). We defined conversion (C+) if a subject progressed to the next syndromal stage within 4 years.ResultsOf 589 participants, 96 (16.3%) were A+T+C+. AD‐RAI performed better than plasma NfL when added on top of clinical features, plasma p‐tau181, and APOE ε4 genotype (area under the curve [AUC] = 0.832 vs. AUC = 0.650 among CU, AUC = 0.853 vs. AUC = 0.805 among MCI) in predicting A+T+C+.DiscussionAD‐RAI outperformed plasma NfL in predicting syndromal conversion of early AD.Highlights
AD‐RAI outperformed plasma NfL in predicting syndromal conversion among early AD.
AD‐RAI showed better metrics than volumetric hippocampal measures in predicting syndromal conversion.
Combining clinical features, plasma p‐tau181 and apolipoprotein E (APOE) with AD‐RAI is the best model for predicting syndromal conversion.
LC–MS/MS bioanalytical method for the quantitative analysis of nifedipine, bisoprolol, and captopril in human plasma: Application to pharmacokinetic studies
Authors: Abdel‐Megied Ahmed M. , Kovalenko Sergiy , Elbarbry Fawzy A. , Piponski Marjan , Oleshchuk Oleksandra , El Deeb Sami , Magdy Galal , Belal Fathalla , Grochovuy Taras , Logoyda Liliya 
Published: April 2023

AbstractIn this study, the development and validation of an accurate and highly sensitive LC–MS/MS method were performed for the estimation of nifedipine, bisoprolol and captopril in real human plasma. Liquid–liquid extraction using tert‐butyl methyl ether was efficiently applied for extraction of the analytes from plasma samples. The chromatographic separation was carried out using an isocratic elution mode on the X‐terra MS C18 column (4.6 × 50 mm, 3.5 μm). The mobile phase consisted of methanol–0.1% formic acid (95:5, v/v) for determination of nifedipine and bisoprolol and acetonitrile–0.1% formic acid (70:30, v/v) for determination of captopril with a flow rate of 0.5 ml/min. Acceptable results regarding the different validation characteristics of the analytes were obtained in accordance with US Food and Drug Administration recommendations for bioanalytical methods. The developed approach was linear over concentration ranges of 0.5–130.0, 50.0–4,500.0 and 0.3–30.0 ng/ml for nifedipine, captopril and bisoprolol, respectively. The method revealed a sufficient lower limit of quantification in the range of 0.3–50.0 ng/ml, as well as high recovery percentages, indicating high bioanalytical applicability. The proposed method was efficiently applied to a pharmacokinetic evaluation of a fixed‐dose combination of the analytes in healthy male volunteers.
Acellular dermal matrix for the treatment of multiple gingival recession defects associated with carious and previously restored cervical lesions: A case report with 10 years of follow‐up
Authors: Boeriu Sorin , Steigmann Larissa , Di Gianfilippo Riccardo 
Published: April 2023

AbstractBackgroundLimited evidence exists on the outcome of the modified coronally advanced tunnel (MCAT) with acellular dermal matrix (ADM) for the treatment of gingival recession defects (GRD) especially when complicated by restored cervical lesions. Therefore, the aim of this case report was to assess the short‐ and long‐term clinical outcomes of maxillary Type 1 recession defects (RT1) associated with restored cervical lesions treated with MCAT with ADM.Methods and ResultsA 43‐year‐old female patient, presented with multiple adjacent RT1 recessions in the left maxilla, previously treated with overhanging cervical resin restorations. The case was approached with a careful evaluation of the diagnostic determinants of root coverage, removal of the aberrant resin restorations, treatment with MCAT with ADM, and periodical evaluation over a follow‐up of 10 years.The treatment was followed by complete root coverage, improvement of gingival phenotype, and perfect root coverage esthetic score. Outcomes were periodically assessed and were maintained over 10 years of follow‐up.ConclusionMCAT with ADM is an effective technique for the treatment of multiple GRD complicated by cervical restorations. Complete root coverage and excellent esthetic outcomes were obtained and maintained in the long term.
Paraneoplastic syndrome of arthropathies as presenting sign for non‐small cell lung cancer
Authors: Lee Alexander Kyle , Singh Gurpreet , Lewis Cleveland W. 
Published: April 2023

AbstractThe aim of this study is to showcase an example of paraneoplastic syndrome, so other healthcare providers can be aware of early NSCLC manifestations. This is also important so providers do not dismiss review of symptoms that may not seem pertinent.
A case of fatal disseminated adenovirus and drug‐resistant Pneumocystis pneumonia in a patient who received chemotherapy for mantle cell lymphoma
Authors: Yu Bo , Saravanan Lakshmi , Tran Dena H. , Davies Alexander J. , Kaur Harpreet , Naraynanan Shivakumar , Verceles Avelino C. , Kim Hyeong J. 
Published: April 2023

AbstractAdenovirus (ADV) may cause severe complications in hematopoietic stem cell transplant recipients, but disseminated ADV infections in patients who received chemotherapy alone for hematological malignancies are poorly understood due to the rarity of cases. Concomitant infection with Pneumocystis (PCP) is extremely rare. Despite being diagnostically challenging, a more specific workup needs to be initiated with a low threshold in patients who are exposed to agents with the potential to suppress T cells. We report a fatal case of disseminated ADV and drug‐resistant PCP pneumonia in a patient with mantle cell lymphoma who had only received combination chemotherapy. A 75‐year‐old man who was diagnosed with mantle cell lymphoma 10 months prior was admitted for mild hypoxic respiratory failure. Bendamustine, Rituximab, Cytarabine regimen had resulted in complete remission of his lymphoma, with the last cycle of chemotherapy administered 3 months prior to admission. CT of the chest revealed ground‐glass opacities concerning pneumonia. Initial laboratory tests were remarkable for mild leukopenia. The respiratory viral panel was only positive for ADV. He did not respond to empiric antibiotics for community‐acquired pneumonia and Trimethoprim / Sulfamethoxazole given later for positive Beta D Glucan (BDG) suggestive of Pneumocystis pneumonia. Then, he developed hemorrhagic cystitis, followed by liver and renal function derangement that prompted checking serum ADV viral load by polymerase chain reaction (PCR). This test took 1 week to return, with a viral load of 50, 000 copies/mL suggesting disseminated ADV infection. Despite initiation of Cidofovir, multi‐organ failure continued to progress, and the follow‐up viral load had doubled on Day 2. The patient passed away the same day shortly after transition to comfort care. T cell suppression seems to be a risk factor for disseminated ADV disease. Clinicians may need to maintain a low threshold to send serum quantitative ADV PCR when symptoms are not improved by antimicrobial treatment for more conventional infections in patients who received agents that are known to suppress T cells, such as Bendamustine.
Cutaneous vasculitis after COVID‐19 vaccination in a 41‐year‐old male
Authors: Pournazari Mehran , Assar Shirin , Farsad Faraneh , Mohamadzadeh Dena 
Published: April 2023

AbstractLeukocytoclastic vasculitis could be a possible adverse event of different SARS‐CoV‐2 vaccines. Clinicians and manufacturers should be aware of this adverse event for appropriate diagnosis and treatment.
A case of superior mesenteric artery syndrome characterized by deep forward bending posture in a cross‐legged position on the floor
Authors: Isono Hiroki , Nakanishi Koki 
Published: April 2023

Key Clinical MessageA 17‐year‐old healthy male presents with severe epicardial pain and frequent vomiting 1 h after lunch, preferring to sit cross‐legged on a stretcher with a deep forward bending posture along with difficulty lying down. SMA syndrome should be considered in the differential diagnosis of patients demonstrating such posture.
Peroxymonosulfate‐Assisted Phenol Degradation via a Magnetic Covalent‐Triazine‐Framework‐Based Photocatalyst
Authors: Ai Lvye , Wang Qian , Cui Fuzhi , Jiang Guofang 
Published: April 2023

AbstractThe development of efficient heterostructures combining covalent organic frameworks (COFs) and ideal semiconductors can significantly improve photocatalytic performance for pollutant degradation. Herein, we present the design, synthesis, and characterization of a core‐shell‐structured nanocomposite comprising covalent triazine framework‐encased Fe3O4 magnetic particles employed as a heterojunction photocatalyst for activating peroxymonosulfate (PMS) in phenol degradation. The distinctive internal structure between the TpMa shell (Tp=2,4,6‐trihydroxy‐1,3,5‐benzenetricarboxaldehyde, Ma=melamine) and the Fe3O4 core (Fe3O4@TpMa) facilitated charge transfer and accelerated charge separation. Furthermore, PMS served as an electron acceptor, enhancing photogenerated charge separation and maximizing the production of reactive oxygen species. The Fe3O4@TpMa/PMS system demonstrated remarkable photocatalytic performance and stability, achieving complete phenol degradation (10 mg L−1) in 40 min. The exceptional photocatalytic activity resulted from the synergistic effect of ⋅OH, SO4⋅−, O2⋅−, 1O2, and h+ generated in the Fe3O4@TpMa/PMS system during the degradation process. Overall, this material offers excellent potential for solar‐driven pollutant degradation and enables the development of COF‐based materials for wastewater treatment applications.
Catalytic Promiscuity of Horseradish Peroxidase: Aerobic Oxidative Regeneration of Oxidized Nicotinamide Cofactors
Authors: Dai Jian‐Rong , Sha M. S. Jiang‐Tao , Zong Min‐Hua , Li Ning 
Published: April 2023

AbstractNAD(P)+ regeneration is of great importance for dehydrogenase (DH)‐catalyzed oxidations. In this work, we report horseradish peroxidase (HRP)‐catalyzed aerobic oxidation of the reduced nicotinamide cofactors, including natural and non‐natural ones, into the oxidized form at neutral and alkaline pH enabled by external amino acids (AAs). HRP showed promiscuous yet extremely low oxidase activity toward NADH at pH 7 in the absence of both AAs and mediators, as evidenced by the observation of compound III. The mechanistic study revealed that AAs as decoy molecules (likely act as acid‐base catalysts) and mediators significantly promoted the formation of compound III as well as its decay back to the ground state. The applicability of the aerobic cofactor regeneration method was demonstrated by the HRP total turnover number up to 88 000 as well as high yields (91–99 %) in bi‐enzymatic synthesis of carboxylic acids. The present work offers an alternative method to recycle NAD(P)+. Also, it may open up possibilities for HRP‐catalyzed aerobic oxidations.
Influencing factors of kinesiophobia in older patients with chronic heart failure: A structural equation model
Authors: Qin Jingwen , Xiong Juanjuan , Chen Chen , Wang Xue , Gao Ya , Zhou Yan , Zheng Guixiang , Gong Kaizheng 
Published: April 2023

AbstractBackgroundOur recent study has demonstrated that kinesiophobia is common in Chinese inpatients with chronic heart failure (CHF). Symptoms of heart failure (HF), coping mode, self‐efficacy for exercise (SEE), and social support have been reported to be associated with kinesiophobia. However, little is known about the relationships between these four variables and kinesiophobia in older patients with CHF.ObjectiveTo test a model of influencing factors of kinesiophobia in older CHF patients.MethodsA cross‐sectional design was conducted from January 2021 to October 2021. The general information questionnaire, the Chinese version of the Tampa Scale for Kinesiophobia Heart (TSK‐SV Heart‐C), Symptom Status Questionnaire‐Heart Failure, SEE, the Medical Coping Modes Questionnaire, and Social Support Rating Scale were used. Spearman correlation analysis and structural equation model (SEM) were performed for data analysis.ResultsA total of 270 older patients with CHF were recruited. Symptom status of HF (r = 0.455, p < .01), avoidance coping mode (r = 0.393, p <.01), and yielding coping mode (r = 0.439, p < .01) were positively correlated with kinesiophobia, while SEE (r = −0.530, p < .01), facing coping mode (r = −0.479, p < .01), and social support (r = −0.464, p < .01) were negatively correlated with kinesiophobia. SEM analysis showed that social support could affect kinesiophobia through the mediating variables of symptom status of HF, avoidance coping mode, and exercise self‐efficacy.ConclusionsSymptoms of HF, coping mode, SEE, and social support may play role in kinesiophobia in older CHF patients. We should pay more attention to the synergies among these four variables in the improvement of kinesiophobia.
Ammonia Synthesis via an Associative Mechanism on Alkaline Earth Metal Sites of Ca3CrN3H
Authors: Cao Yu , Toshcheva Ekaterina , Almaksoud Walid , Ahmad Rafia , Tsumori Tatsuya , Rai Rohit , Tang Ya , Cavallo Luigi , Kageyama Hiroshi , Kobayashi Yoji 
Published: April 2023

AbstractTypically, transition metals are considered as the centers for the activation of dinitrogen. Here we demonstrate that the nitride hydride compound Ca3CrN3H, with robust ammonia synthesis activity, can activate dinitrogen through active sites where calcium provides the primary coordination environment. DFT calculations also reveal that an associative mechanism is favorable, distinct from the dissociative mechanism found in traditional Ru or Fe catalysts. This work shows the potential of alkaline earth metal hydride catalysts and other related 1 D hydride/electrides for ammonia synthesis.
A systematic literature review of machine learning applications in IoT
Authors: Gherbi Chirihane , Senouci Oussama , Harbi Yasmine , Medani Khedidja , Aliouat Zibouda 
Published: April 2023

SummaryThe Internet of Things (IoT) is a network of interconnected smart objects having capabilities that collectively form an ecosystem and enable the delivery of smart services to users. The IoT is providing several benefits into people's lives through the environment. The various applications that are run in the IoT environment offer facilities and services. The most crucial services provided by IoT applications are quick decision for efficient management. Recently, machine learning (ML) techniques have been successfully used to maximize the potential of IoT systems. This paper presents a systematic review of the literature on the integration of ML methods in the IoT. The challenges of IoT systems are split into two categories: fundamental operation and performance. We also look at how ML is assisting in the resolution of fundamental system operation challenges such as security, big data, clustering, routing, and data aggregation.
Toxicological Impacts of Microplastics: Effects on Levels of Cellular Thiols in Mytilus galloprovincialis
Authors: Murano Carola , Palumbo Anna , Leone Serena 
Published: April 2023

Abstract
Over the last decade, the biological impact of microplastics in marine environments has become a serious global concern. Beyond their biological complexity, it is believed that many lethal and sublethal effects related to microplastic toxicity are triggered by oxidative stress and subsequently activated pathways. Thus, for marine organisms, having efficient mechanisms to fight the accumulation of oxidizing agents becomes indispensable to counteract the effects of microplastics. To date, our knowledge of the physiological effects of microplastics and the response of the antioxidant system in benthic species remains limited. The purpose of our study was to investigate the effects of short-term exposure on the levels of two fundamental nonprotein antioxidants, glutathione (GSH) and ovothiol (OSH), in different tissues of Mytilus galloprovincialis. Our results show that the metabolism of OSH and GSH in mussels is clearly affected by acute microplastic exposure, and that the antioxidant response differs based on sex and reproductive stage. Indeed, although in the reproductive season the overall levels of GSH and OSH in different tissues significantly increase with respect to the control condition, the antioxidant response of the organisms, especially males, in the spent stage often exhibits a biphasic U-shaped dose–response effect. Our study represents a pivotal investigation on the effects of microplastic exposure on the pools of two fundamental cellular antioxidants, with potential ecodiagnostic implications to forecast the stress status after exposure to microplastics, and highlights the possibility that the effects of these contaminants may vary over time based on the physiological condition of the animals. Environ Toxicol Chem 2023;42:1607–1613. © 2023 SETAC