bluuebunny/crossref_metadata_2025_split huggingface dataset:
Hippocampal delivery of neurotrophic factor-α1/carboxypeptidase E gene prevents neurodegeneration, amyloidosis, memory loss in Alzheimer’s Disease male mice
Authors: Xiao Lan , Yang Xuyu , Sharma Vinay Kumar , Abebe Daniel , Loh Y. Peng 
Published: June 2023

AbstractAlzheimer’s Disease (AD) is a prevalent neurodegenerative disease characterized by tau hyperphosphorylation, Aβ1-42 aggregation and cognitive dysfunction. Therapeutic agents directed at mitigating tau aggregation and clearing Aβ1-42, and delivery of growth factor genes (BDNF, FGF2), have ameliorated cognitive deficits, but these approaches did not prevent or stop AD progression. Here we report that viral-(AAV) delivery of Neurotrophic Factor-α1/Carboxypeptidase E (NF-α1/CPE) gene in hippocampus at an early age prevented later development of cognitive deficits as assessed by Morris water maze and novel object recognition assays, neurodegeneration, and tau hyperphosphorylation in male 3xTg-AD mice. Additionally, amyloid precursor protein (APP) expression was reduced to near non-AD levels, and insoluble Aβ1-42 was reduced significantly. Pro-survival proteins: mitochondrial Bcl2 and Serpina3g were increased; and mitophagy inhibitor Plin4 and pro-inflammatory protein Card14 were decreased in AAV-NF-α1/CPE treated versus untreated AD mice. Thus NF-α1/CPE gene therapy targets many regulatory components to prevent cognitive deficits in 3xTg-AD mice and has implications as a new therapy to prevent AD progression by promoting cell survival, inhibiting APP overexpression and tau hyperphosphorylation.
Recent progress in the effect of magnetic iron oxide nanoparticles on cells and extracellular vesicles
Authors: Chen Yuling , Hou Shike 
Published: June 2023

AbstractAt present, iron oxide nanoparticles (IONPs) are widely used in the biomedical field. They have unique advantages in targeted drug delivery, imaging and disease treatment. However, there are many things to pay attention to. In this paper, we reviewed the fate of IONPs in different cells and the influence on the production, separation, delivery and treatment of extracellular vesicles. It aims to provide cutting-edge knowledge related to iron oxide nanoparticles. Only by ensuring the safety and effectiveness of IONPs can their application in biomedical research and clinic be further improved.
A salivary GMC oxidoreductase of Manduca sexta re-arranges the green leaf volatile profile of its host plant
Authors: Lin Yu-Hsien , Silven Juliette J. M. , Wybouw Nicky , Fandino Richard A. , Dekker Henk L. , Vogel Heiko , Wu Yueh-Lung , de Koster Chris , Große-Wilde Ewald , Haring Michel A. , Schuurink Robert C. , Allmann Silke 
Published: June 2023

AbstractGreen leaf volatiles (GLVs) are short-chain oxylipins that are emitted from plants in response to stress. Previous studies have shown that oral secretions (OS) of the tobacco hornworm Manduca sexta, introduced into plant wounds during feeding, catalyze the re-arrangement of GLVs from Z-3- to E-2-isomers. This change in the volatile signal however is bittersweet for the insect as it can be used by their natural enemies, as a prey location cue. Here we show that (3Z):(2E)-hexenal isomerase (Hi-1) in M. sexta’s OS catalyzes the conversion of the GLV Z-3-hexenal to E-2-hexenal. Hi-1 mutants that were raised on a GLV-free diet showed developmental disorders, indicating that Hi-1 also metabolizes other substrates important for the insect’s development. Phylogenetic analysis placed Hi-1 within the GMCβ-subfamily and showed that Hi-1 homologs from other lepidopterans could catalyze similar reactions. Our results indicate that Hi-1 not only modulates the plant’s GLV-bouquet but also functions in insect development.
Natural short-lived halogens exert an indirect cooling effect on climate
Authors: Saiz-Lopez Alfonso , Fernandez Rafael P. , Li Qinyi , Cuevas Carlos A. , Fu Xiao , Kinnison Douglas E. , Tilmes Simone , Mahajan Anoop S. , Gómez Martín Juan Carlos , Iglesias-Suarez Fernando , Hossaini Ryan , Plane John M. C. , Myhre Gunnar , Lamarque Jean-François 
Published: June 2023

AbstractObservational evidence shows the ubiquitous presence of ocean-emitted short-lived halogens in the global atmosphere1–3. Natural emissions of these chemical compounds have been anthropogenically amplified since pre-industrial times4–6, while, in addition, anthropogenic short-lived halocarbons are currently being emitted to the atmosphere7,8. Despite their widespread distribution in the atmosphere, the combined impact of these species on Earth’s radiative balance remains unknown. Here we show that short-lived halogens exert a substantial indirect cooling effect at present (−0.13 ± 0.03 watts per square metre) that arises from halogen-mediated radiative perturbations of ozone (−0.24 ± 0.02 watts per square metre), compensated by those from methane (+0.09 ± 0.01 watts per square metre), aerosols (+0.03 ± 0.01 watts per square metre) and stratospheric water vapour (+0.011 ± 0.001 watts per square metre). Importantly, this substantial cooling effect has increased since 1750 by −0.05 ± 0.03 watts per square metre (61 per cent), driven by the anthropogenic amplification of natural halogen emissions, and is projected to change further (18–31 per cent by 2100) depending on climate warming projections and socioeconomic development. We conclude that the indirect radiative effect due to short-lived halogens should now be incorporated into climate models to provide a more realistic natural baseline of Earth’s climate system.
GDF15 promotes weight loss by enhancing energy expenditure in muscle
Authors: Wang Dongdong , Townsend Logan K. , DesOrmeaux Geneviève J. , Frangos Sara M. , Batchuluun Battsetseg , Dumont Lauralyne , Kuhre Rune Ehrenreich , Ahmadi Elham , Hu Sumei , Rebalka Irena A. , Gautam Jaya , Jabile Maria Joy Therese , Pileggi Chantal A. , Rehal Sonia , Desjardins Eric M. , Tsakiridis Evangelia E. , Lally James S. V. , Juracic Emma Sara , Tupling A. Russell , Gerstein Hertzel C. , Paré Guillaume , Tsakiridis Theodoros , Harper Mary-Ellen , Hawke Thomas J. , Speakman John R. , Blondin Denis P. , Holloway Graham P. , Jørgensen Sebastian Beck , Steinberg Gregory R. 
Published: June 2023

AbstractCaloric restriction that promotes weight loss is an effective strategy for treating non-alcoholic fatty liver disease and improving insulin sensitivity in people with type 2 diabetes1. Despite its effectiveness, in most individuals, weight loss is usually not maintained partly due to physiological adaptations that suppress energy expenditure, a process known as adaptive thermogenesis, the mechanistic underpinnings of which are unclear2,3. Treatment of rodents fed a high-fat diet with recombinant growth differentiating factor 15 (GDF15) reduces obesity and improves glycaemic control through glial-cell-derived neurotrophic factor family receptor α-like (GFRAL)-dependent suppression of food intake4–7. Here we find that, in addition to suppressing appetite, GDF15 counteracts compensatory reductions in energy expenditure, eliciting greater weight loss and reductions in non-alcoholic fatty liver disease (NAFLD) compared to caloric restriction alone. This effect of GDF15 to maintain energy expenditure during calorie restriction requires a GFRAL–β-adrenergic-dependent signalling axis that increases fatty acid oxidation and calcium futile cycling in the skeletal muscle of mice. These data indicate that therapeutic targeting of the GDF15–GFRAL pathway may be useful for maintaining energy expenditure in skeletal muscle during caloric restriction.
Potency of some economic variables affecting environmental quality in Nigeria
Authors: Ogunbode T. O. , Jazat J. P. , Akande J. A. 
Published: June 2023

AbstractEnvironmental quality (EQ) is germane to achieving sustainable living on earth. To evaluate a related stimulus of EQ on area basis, a comparative analyses of economic factors that influence pollution in Iwo and Ibadan metropolis was carried out. Data for the study was generated through the administration of 700 structured questionnaires in total at both locations, out of which 165 and 473 were retrieved from Iwo and Ibadan respectively. The distribution of respondents in form of male gender, married status, tertiary education and household size of not more than 5 were 51.5%, 78.2%, 24.9% and 46.1% respectively for Iwo, while it was 38.5%, 81.0%, 28.6% and 48.8% in the same order for Ibadan. Economic factors analyzed were (1) Income (2) living standard indexed by the form of accommodation occupied (3) waste/noise management regimen (4) energy utilization (5) orthodox versus green economy adoption and (6) waste sorting capacity. Kaiser–Meyer–Olkin sampling adequacy and Bartlett’s test of sphericity admitted the data as factorable at p < 0.005. Results showed that three of the economic variables significantly explained the pollution status in Iwo and Ibadan. The variables in Iwo explained 59.3% of the factors and these are waste/noise management strategies (22.5%), living standard (18.7%) and green economy adoption (18.3%). 60.2% of economic impacts on pollution in Ibadan were explained by living standard (24.4%), green economy adoption (18.8%) and waste/noise management strategies (17.0%). Only two of the variables namely living standard, and green economy adoption were common to the two study locations, although, their importance and ordering varied. While waste and noise management were most significant in Iwo, the same variable had the least effect in Ibadan. Green economy adoption was least in Iwo but most significant in Ibadan. Thus, the economic factors influencing pollution in Iwo and Ibadan metropolis, though similar, may not be given a generalized weighting. In other words, analyses of pollution-related matters from the economic viewpoint should be location specific.
Development of a human malaria-on-a-chip disease model for drug efficacy and off-target toxicity evaluation
Authors: Rupar Michael J. , Sasserath Trevor , Smith Ethan , Comiter Brandon , Sriram Narasimhan , Long Christopher J. , McAleer Christopher W. , Hickman James J. 
Published: June 2023

AbstractA functional, multi-organ, serum-free system was developed for the culture of P. falciparum in an attempt to establish innovative platforms for therapeutic drug development. It contains 4 human organ constructs including hepatocytes, splenocytes, endothelial cells, as well as recirculating red blood cells which allow for infection with the parasite. Two strains of P. falciparum were used: the 3D7 strain, which is sensitive to chloroquine; and the W2 strain, which is resistant to chloroquine. The maintenance of functional cells was successfully demonstrated both in healthy and diseased conditions for 7 days in the recirculating microfluidic model. To demonstrate an effective platform for therapeutic development, systems infected with the 3D7 strain were treated with chloroquine which significantly decreased parasitemia, with recrudescence observed after 5 days. Conversely, when the W2 systems were dosed with chloroquine, parasitemia levels were moderately decreased when compared to the 3D7 model. The system also allows for the concurrent evaluation of off-target toxicity for the anti-malarial treatment in a dose dependent manner which indicates this model could be utilized for therapeutic index determination. The work described here establishes a new approach to the evaluation of anti-malarial therapeutics in a realistic human model with recirculating blood cells for 7 days.
Declining incidence of cerebral palsy in South Korea
Authors: Kim Gun-Ha , Lee Gisu , Ha Sungyeon , Cho Geum Joon , Kim Yoon Ha 
Published: June 2023

AbstractPresuming that the incidence of cerebral palsy (CP) in Korea is decreasing due to medical advances, we analyzed the trends and risk factors of CP in changing circumstances. We identified all women who delivered a singleton between 2007 and 2015 using the Korea National Health Insurance (KNHI). Information on pregnancy and birth was obtained by linking the KNHI claims database and data from the national health-screening program for infants and children. The 4-years incidence of CP decreased significantly from 4.77 to 2.52 per 1000 babies during the study period. The multivariate analysis revealed that the risk of developing CP was 29.5 times higher in preterm infants born before 28 weeks of gestational age, 24.5 times higher in infants born between 28 and 34 weeks, and 4.5 times higher in infants born between 34 and 36 weeks, compared to full-term appropriate for age (2.5 ~ 4 kg of body weight) infants. 5.6 times higher in those with birth weight < 2500 g, and 3.8 times higher in pregnancies with polyhydramnios. Additionally, respiratory distress syndrome increased the risk of developing CP by 2.04 times, while necrotizing enterocolitis was associated with a 2.80-fold increased risk of CP. In Korea, the incidence of CP in singleton decreased from 2007 to 2015. We need to continue to focus on developing medical technologies for the early detection of high-risk neonates and minimizing brain damage to reduce the incidence rate of CP effectively.
A novel approach for the analysis of single-cell RNA sequencing identifies TMEM14B as a novel poor prognostic marker in hepatocellular carcinoma
Authors: Ma Ding , Liu Shuwen , He Qinyu , Kong Lingkai , Liu Kua , Xiao Lingjun , Xin Qilei , Bi Yanyu , Wu Junhua , Jiang Chunping 
Published: June 2023

AbstractA fundamental goal in cancer-associated genome sequencing is to identify the key genes. Protein–protein interactions (PPIs) play a crucially important role in this goal. Here, human reference interactome (HuRI) map was generated and 64,006 PPIs involving 9094 proteins were identified. Here, we developed a physical link and co-expression combinatory network construction (PLACE) method for genes of interest, which provides a rapid way to analyze genome sequencing datasets. Next, Kaplan‒Meier survival analysis, CCK8 assays, scratch wound assays and Transwell assays were applied to confirm the results. In this study, we selected single-cell sequencing data from patients with hepatocellular carcinoma (HCC) in GSE149614. The PLACE method constructs a protein connection network for genes of interest, and a large fraction (80%) of the genes (screened by the PLACE method) were associated with survival. Then, PLACE discovered that transmembrane protein 14B (TMEM14B) was the most significant prognostic key gene, and target genes of TMEM14B were predicted. The TMEM14B-target gene regulatory network was constructed by PLACE. We also detected that TMEM14B-knockdown inhibited proliferation and migration. The results demonstrate that we proposed a new effective method for identifying key genes. The PLACE method can be used widely and make outstanding contributions to the tumor research field.
Complementary use of statistical parametric mapping and gait profile score to describe walking alterations in multiple sclerosis: a cross-sectional study
Authors: Mestanza Mattos Fabiola Giovanna , Luciano Francesco , Lencioni Tiziana , Gervasoni Elisa , Jonsdottir Johanna , Anastasi Denise , Pavei Gaspare , Clerici Mario , Cattaneo Davide 
Published: June 2023

AbstractGait analysis is often used to study locomotor alterations in people with multiple sclerosis (PwMS), but the large number of extracted variables challenges the interpretability. In this paper, we analysed gait alterations by combining the Gait Profile Score (GPS), which summarizes kinematic locomotor deviations, and Statistical Parametric Mapping (SPM), which compares kinematics and kinetics over the whole gait cycle. Eleven PwMS and 11 speed-matched Healthy Controls (HC) underwent overground gait analysis. GPS were compared through independent-samples t-tests; sagittal-plane kinematics and power at hip, knee, and ankle were compared through SPM Hotelling’s-T2 and SPM t-tests. Spearman’s correlation coefficients (r) between GPS and clinical outcomes were also calculated. PwMS had higher GPS than HC (PwMS = 8.74 ± 2.13°; HC = 5.01 ± 1.41°;p < 0.001). Multivariate SPM found statistically significant differences at 0–49%, 70–80%, and 93–99% of stride (p < 0.05) and univariate analysis showed reduced ankle dorsiflexion, and lower knee flexion during pre-swing and swing. GPS correlated with Expanded Disability Status Scale (r = 0.65; 95%C.I.[0.04,0.91]; p = 0.04) and 2-Minute Walking Test (r = -0.65; 95%C.I.[-0.91,-0.04]; p = 0.04). GPS in conjunction with SPM revealed multi-joint kinematic alterations on sagittal plane involving distal joint angles, ankle and knee, during the stance phase with no changes at the proximal level. Gait deviations were more pronounced in PwMS with higher disability and walking limitations.
Virtual social interactions during the COVID-19 pandemic: the effect of interpersonal motor synchrony on social interactions in the virtual space
Authors: Gvirts Hila , Ehrenfeld Lya , Sharma Mini , Mizrahi Moran 
Published: June 2023

AbstractAlthough the link between motor synchrony and emotional alignment has been extensively studied during face-to-face interaction, the question of whether this association also exists in virtual settings has remained unanswered. Here, we examined whether this link exists during virtual social interactions and whether pro-social effects will be induced during those interactions. To this end, two strangers shared difficulties they have experienced due to the COVID-19 pandemic during a virtual social interaction that included both audio and video. The findings revealed that motor synchrony and emotional alignment can arise spontaneously during a virtual social interaction between two strangers. Moreover, this interaction yielded a decrease in negative affect and an increase in positive affect, as well as an increase in feelings of trust, liking, cohesion, self-other overlap, and similarity between the strangers. Finally, a higher level of synchrony during the virtual interaction was specifically associated with increased positive emotional alignment and liking. It can thus be presumed that virtual social interactions may share similar characteristics and social effects as face-to-face interactions. Considering the tremendous changes the COVID-19 pandemic has caused regarding social communication, these findings may provide grounds for developing new intervention protocols aimed at dealing with the consequences of social distancing.
Deep learning and ultrasound feature fusion model predicts the malignancy of complex cystic and solid breast nodules with color Doppler images
Authors: Liu Han , Hou Chun-Jie , Tang Jing-Lan , Sun Li-Tao , Lu Ke-Feng , Liu Ying , Du Pei 
Published: June 2023

AbstractThis study aimed to evaluate the performance of traditional-deep learning combination model based on Doppler ultrasound for diagnosing malignant complex cystic and solid breast nodules. A conventional statistical prediction model based on the ultrasound features and basic clinical information was established. A deep learning prediction model was used to train the training group images and derive the deep learning prediction model. The two models were validated, and their accuracy rates were compared using the data and images of the test group, respectively. A logistic regression method was used to combine the two models to derive a combination diagnostic model and validate it in the test group. The diagnostic performance of each model was represented by the receiver operating characteristic curve and the area under the curve. In the test cohort, the diagnostic efficacy of the deep learning model was better than traditional statistical model, and the combined diagnostic model was better and outperformed the other two models (combination model vs traditional statistical model: AUC: 0.95 > 0.70, P = 0.001; combination model vs deep learning model: AUC: 0.95 > 0.87, P = 0.04). A combination model based on deep learning and ultrasound features has good diagnostic value.
Tuning of the Brillouin scattering properties in microstructured optical fibers by liquid infiltration
Authors: Catalano Ester , Vallifuoco Raffaele , Zeni Luigi , Dufour Alexis , Marin Emmanuel , Girard Sylvain , Minardo Aldo 
Published: June 2023

AbstractWe demonstrate the possibility to modify the Brillouin scattering properties of a microstructured pure-silica core optical fiber, by infiltrating a liquid inside its holes. In particular, we show that the dependence of the Brillouin frequency shift (BFS) on the temperature can be reduced by infiltration, owing to the large negative thermo-optic coefficient of the liquid. Infiltrating a chloroform-acetonitrile mixture with a refractive index of 1.365 inside the holes of a suspended-core fiber with a core diameter of 3 µm, the BFS temperature sensing coefficient is reduced by ≈ 21%, while the strain sensitivity remains almost unaltered. Besides tuning the temperature sensing coefficient, the proposed platform could find other applications in Brillouin sensing, such as distributed electrical and magnetic measurements, or enhanced Brillouin gain in fibers infiltrated with high nonlinear optical media.
Generation of potentially inhibitory autoantibodies to ADAMTS13 in coronavirus disease 2019
Authors: Doevelaar Adrian A. N. , Bachmann Martin , Hölzer Bodo , Seibert Felix S. , Rohn Benjamin J. , Zgoura Panagiota , Witzke Oliver , Dittmer Ulf , Brenner Thorsten , Paniskaki Krystallenia , Yilmaz Serap , Dittmer Rita , Schneppenheim Sonja , Wilhelm Jochen , Stervbo Ulrik , Babel Nina , Budde Ulrich , Westhoff Timm H. 
Published: June 2023

AbstractIt has recently been shown that von Willebrand factor (VWF) multimers contribute to immunothrombosis in Coronavirus disease 2019 (COVID-19). Since COVID-19 is associated with an increased risk of autoreactivity, the present study investigates, whether the generation of autoantibodies to ADAMTS13 contributes to this finding. In this observational prospective controlled multicenter study blood samples and clinical data of patients hospitalized for COVID-19 were collected from April to November 2020. The study included 156 individuals with 90 patients having confirmed COVID-19 of mild to critical severity. 30 healthy individuals and 36 critically ill ICU patients without COVID-19 served as controls. ADAMTS13 antibodies occurred in 31 (34.4%) COVID-19 patients. Antibodies occurred more often in critically ill COVID-19 patients (55.9%) than non-COVID-19 ICU patients and healthy controls (5.6% and 6.7%; p < 0.001), respectively. Generation of ADAMTS13 antibodies in COVID-19 was associated with lower ADAMTS13 activity (56.5%, interquartile range (IQR) 21.25 vs. 71.5%, IQR 24.25, p = 0.0041), increased disease severity (severe or critical in 90% vs. 62.3%, p = 0.019), and a trend to higher mortality (35.5% vs. 18.6%, p = 0.077). Median time to antibody development was 11 days after first positive SARS-CoV-2-PCR specimen. Gel analysis of VWF multimers resembled the constellation in patients with TTP. The present study demonstrates for the first time, that generation of ADAMTS13 antibodies is frequent in COVID-19, associated with lower ADAMTS13 activity and increased risk of an adverse disease course. These findings provide a rationale to include ADAMTS13 antibodies in the diagnostic workup of SARS-CoV-2 infections.