bluuebunny/crossref_metadata_2025_split huggingface dataset:
Impact of the Surgical Approach for Neoadjuvantly Treated Gastro-Esophageal Junction type II Tumors- a Multi-National, High-Volume Center Retrospective Cohort Analysis
Authors: Wirsik Naita M. , Schmidt Thomas , Nienhüser Henrik , Donlon Noel E. , de Jongh Cas , Uzun Eren , Fuchs Hans F. , Brunner Stefanie , Alakus Hakan , Grimminger Peter , Schneider Martin , Reynolds John V. , van Hillegersberg Richard , Bruns Christiane J. 
Published: July 2023

Objective:To explore oncologic outcomes of transhiatal gastrectomy (THG) or transthoracic esophagectomy (TTE) for neoadjuvantly treated gastroesophageal junction (GEJ) Siewert type II adenocarcinomas, a multi-national, high-volume center cohort analysis was undertaken.Background:Neoadjuvant radiochemotherapy (RCTx) or perioperative chemotherapy (CTx) followed by surgery is the standard therapy for locally advanced GEJ. However, the optimal surgical approach for type II GEJ tumors remains unclear, as the decision is mainly based on individual experience and assessment of operative risk.Methods:A retrospective analysis of five prospectively maintained databases was conducted. Between 2012-2021, 800 patients fulfilled inclusion criteria for type II GEJ tumors and neoadjuvant RCTx or CTx. The primary endpoint was median overall survival (mOS). Propensity score matching (PSM) was performed to minimize selection bias.Results:Patients undergoing THG (n=163, 20.4%) had higher ASA classification and cT-stage (P<0.001) than patients undergoing TTE (n=637, 79.6%). Neoadjuvant therapy was different as the THG-group were mainly undergoing CTx (87.1%,P<0.001). The TTE-group showed higher tumor regression (P=0.009), lower ypT-/ypM-categories (bothP<0.001), higher nodal yield (P=0.009) and higher R0-resection rate (P=0.001). The mOS after TTE was longer (78.0 versus 40.0 months (P=0.013). After PSM a higher R0-resection rate (P=0.004) and mOS benefit after TTE remained (P=0.04). Subgroup analyses of patients without distant metastasis (P=0.037) and patients only after neoadjuvant chemotherapy (P=0.021) confirmed the survival benefit of TTE. TTE was an independent predictor of longer survival.Conclusions:Awaiting results of the randomized CARDIA-trial, TTE should in high-volume centers be considered the preferred approach due to favorable oncologic outcomes.
Living Donor Liver Transplantation (LDLT) for Hepatocellular Carcinoma (HCC) within and Outside Traditional Selection Criteria
Authors: Ivanics Tommy , Claasen Marco PAW , Samstein Benjamin , Emond Jean C , Fox Alyson N , Pomfret Elizabeth , Pomposelli James , Tabrizian Parissa , Florman Sander S , Mehta Neil , Roberts John P , Emamaullee Juliet A , Genyk Yuri , Hernandez-Alejandro Roberto , Tomiyama Koji , Sasaki Kazunari , Quintini Cristiano , Nagai Shunji , Abouljoud Marwan , Olthoff Kim M , Hoteit Maarouf A , Heimbach Julie , Taner Timucin , Liapakis AnnMarie H , Mulligan David C , Sapisochin Gonzalo , Halazun Karim J 
Published: July 2023

Objective:
We evaluated long-term oncologic outcomes of patients post-LDLT within and outside standard transplant selection criteria and the added value of the incorporation of the New York-California (NYCA) score.

Summary Background Data:
LDLT offers an opportunity to decrease the liver transplant waitlist, reduce waitlist mortality and expand selection criteria for patients with HCC.

Methods:
Primary adult LDLT recipients between Oct-1999 and Aug-2019 were identified from a multicenter cohort of twelve North American centers. Post-transplant and recurrence-free survival were evaluated using the Kaplan-Meier method.

Results:
Three-hundred-and-sixty LDLTs were identified. Patients within Milan criteria (MC) at transplant had a 1-,5-, and 10-year post-transplant survival of 90.9%,78.5%, and 64.1% vs. outside MC 90.4%,68.6%, and 57.7%(P=0.20). For patients within the UCSF criteria, respective post-transplant survival was 90.6%,77.8%, and 65.0%, vs. outside UCSF 92.1%,63.8%, and 45.8%(P=0.08). Fifty-three (83%) patients classified as outside MC at transplant would have been classified as either low- or acceptable risk with the NYCA score. These patients had a 5-year OS of 72.2%. Similarly, 28(80%) patients classified as outside UCSF at transplant would have been classified as low- or acceptable risk with a 5-year OS of 65.3%.

Conclusions:
Long-term survival is excellent for patients with HCC undergoing LDLT within and outside selection criteria, exceeding the minimum recommended 5-years rate of 60% proposed by consensus guidelines. The NYCA categorization offers insight into identifying a substantial proportion of patients with HCC outside the MC and the UCSF criteria who still achieve similar post-LDLT outcomes as patients within the criteria.
Western Diet Induced Transcriptional Changes in Anastomotic Tissue is Associated with Early Local Recurrence in a Mouse Model of Colorectal Surgery
Authors: Vigneswaran Janani , Keskey Robert C. , Morgan Ryan B. , Alverdy John C. , Alpert Lindsay , Chang Eugene , Weichselbaum Ralph , Zaborina Olga , Shogan Benjamin D. 
Published: July 2023

Objective:
To determine the timeframe and associated changes in the microenvironment that promote the development of a diet-induced local-regional recurrence in a mouse model of colorectal surgery.

Summary Background Data:
Postoperative recurrence and metastasis occur in up to 30% of patients undergoing attempted resection for colorectal cancer (CRC). The underlying mechanisms that drive the development of postoperative recurrences are poorly understood. Preclinical studies have demonstrated a diet and microbial-driven pathogenesis of local-regional recurrence, yet the precise mechanisms remain undefined.

Methods:
BALB/C mice were fed a western (WD) or standard diet (SD), underwent a colon resection and anastomosis, given anEnterococcusfaecalis enema on post-operative day (POD) 1, and subjected to a CT26 cancer cell enema (mimicking shed cancer cells) on POD 2. Mice were sacrificed between POD3-7 and cancer cell migration was tracked. Dynamic changes in gene expression of anastomotic tissue that were associated with cancer cell migration was assessed.

Results:
Tumor cells were identified in mice fed either a SD or WD in both anastomotic and lymphatic tissue as early as on POD3. Histology demonstrated that these tumor cells were viable and replicating. In WD fed mice, the number of tumor cells increased over the early perioperative period and was significantly higher than in mice fed a SD. Microarray analysis of anastomotic tissue found that WD fed mice had 11 dysregulated genes associated with tumorigenesis.

Conclusions:
A WD promotes cancer cells to permeate a healing anastomosis and migrate into anastomotic and lymphatic tissue forming viable tumor nodules. These data offer a novel recurrence pathogenesis by which the intestinal microenvironment promotes a CRC local-regional recurrence.
A Prognostic Model To Predict Survival After Recurrence Among Patients With Recurrent Hepatocellular Carcinoma
Authors: Moazzam Zorays , Alaimo Laura , Endo Yutaka , Lima Henrique A. , Woldesenbet Selamawit , Rueda Belisario Ortiz , Yang Jason , Ratti Francesca , Marques Hugo P , Cauchy Francois , Lam Vincent , Poultsides George A , Popescu Irinel , Alexandrescu Sorin , Martel Guillaume , Guglielmi Alfredo , Hugh Tom , Aldrighetti Luca , Shen Feng , Endo Itaru , Pawlik Timothy M 
Published: July 2023

Objective:
We sought to develop and validate a preoperative model to predict survival after recurrence (SAR) in hepatocellular carcinoma (HCC).

Summary Background Data:
Although HCC is characterized by rates of recurrence as high as 60%, models to predict outcomes after recurrence remain relatively unexplored.

Methods:
Patients who developed recurrent HCC between 2000-2020 were identified from an international multi-institutional database. Clinicopathologic data on primary disease, and laboratory and radiologic imaging data on recurrent disease were collected. Multivariable cox regression analysis and internal bootstrap validation (5,000 repetitions) were used to develop and validate the SARScore. Optimal Survival Tree (OST) analysis was used to characterize SAR among patients treated with various treatment modalities.

Results:
Among 497 patients who developed recurrent HCC, median SAR was 41.2 months (95% CI 38.1–52.0). Presence of cirrhosis, number of primary tumors, primary macrovascular invasion, primary R1 resection margin, AFP>400 ng/mL on diagnosis of recurrent disease, radiologic extrahepatic recurrence, radiologic size and number of recurrent lesions, radiologic recurrent bilobar disease and early recurrence (≤24 months) were included in the model. The SARScore successfully stratified 1-, 3- and 5-year SAR and demonstrated strong discriminatory ability (3-year AUC: 0.75, 95% CI 0.70–0.79). While a subset of patients benefitted from resection/ablation, OST analysis revealed that patients with high SARScore disease had the worst outcomes (5-year AUC; training: 0.79 vs. testing: 0.71). The SARScore model was made available online for ease-of-use and clinical applicability (https://yutaka-endo.shinyapps.io/SARScore/).

Conclusion:
The SARScore demonstrated strong discriminatory ability and may be a clinically useful tool to help stratify risk and guide treatment for patients with recurrent HCC.
Different Approaches of Laparoscopic Mesohepatectomy for Centrally Located Hepatocellular Carcinomas
Authors: Hongpeng Chu , Guannan Wang , Shangfei Pan , Zhengchao Shen , Yongyou Wang , Xiaoming Wang 
Published: July 2023

Background:
This study aimed to describe a simple and novel positional classification system for centrally located hepatocellular carcinoma (CL-HCC), based on which different surgical approaches for laparoscopic mesohepatectomy (LMH) were chosen.

Materials and Methods:
The data of patients with CL-HCC who underwent LMH between January 2017 and December 2021 were retrospectively analyzed. The positional classification method was used to locate tumors. In addition, different approaches were used during the surgery according to the classification type.

Results:
All 98 patients underwent LMH, of whom 4 were converted to open surgery. Types I, Ⅱ, and Ⅲ were 24, 37, and 37, respectively. Blood transfusions were performed in only 7 patients, of which the amount was 800 (600, 900) mL. All the patients underwent R0 resection. The complication rate was 9.2% in all cases. No deaths occurred.

Conclusions:
Our proposed classification system and corresponding surgical approach in LMH is conducive to the successful completion of surgery.
Evaluation of Alternate Laparoscopic Stapling Device for Bariatric Surgery
Authors: Walton Gregory F. , Broussard Toby D. 
Published: July 2023

Background:
As laparoscopic bariatric surgical techniques have matured, the incidence of complications has decreased over time. Surgical stapling devices are commonly used for resection and anastomosis of gastric tissue during bariatric surgery. The purpose of this study is to assess and compare complication rates and clinical outcomes between patient cohorts using 2 different approved stapling devices.

Materials and Methods:
Clinical outcomes were retrospectively compared for patients undergoing bariatric surgery between April 2019 and December 2020 using laparoscopic surgical stapling device A (LSSD-A) against outcomes between January 2017 and September 2018 using laparoscopic surgical stapling device B (LSSD-B). Tradenames for LSSD-A and LSSD-B were AEON Endostapler and Endo GIA with Tri-Staple Technology, respectively.

Results:
In all, 814 patients underwent laparoscopic bariatric operation using LSSD-A and 1034 using LSSD-B. Laparoscopic surgery included sleeve gastrectomy (n=1359, 73.5%), Roux-en-Y gastric bypass (RYGB) (n=425, 23.0%), and single anastomosis duodenoileostomy with gastric sleeve (n=64, 3.5%). Stapler-related complications were evenly distributed over the 20.9-month LSSD-B study period. No stapler-related complications were seen in the last 13 months of the LSSD-A study period, during which time 428 (52.5%) laparoscopic bariatric operations were performed. One staple line leak was observed, occurring in the LSSD-B group. Patients undergoing laparoscopic RYGB with LSSD-A had fewer overall complications, fewer transfusions, and fewer reoperations for staple line bleeding.

Conclusions:
Both LSSD-A and LSSD-B were safe and effective in the performance of laparoscopic bariatric operations with low complication rates and comparable clinical performance in sleeve gastrectomy and single anastomosis duodenoileostomy with gastric sleeve. Statistically significant differences in complication rates were observed in RYGB favoring LSSD-A.
Retinal Thickness in Essential Tremor and Early Parkinson Disease: Exploring Diagnostic Insights
Authors: Terravecchia Claudio , Mostile Giovanni , Chisari Clara G. , Rascunà Cristina , Terranova Roberta , Cicero Calogero E. , Giuliano Loretta , Donzuso Giulia , Sciacca Giorgia , Luca Antonina , Preux Pierre-Marie , Jankovic Joseph , Zappia Mario , Nicoletti Alessandra 
Published: July 2023

Background:
Essential tremor (ET) represents a heterogeneous condition which may overlap with Parkinson disease (PD) even at early stages, by sharing some subtle clinical aspects. Longstanding ET demonstrated also higher risk of developing PD, especially with a Tremor-dominant (TD-PD) phenotype. Therefore, differential diagnosis between ET and early PD could be quite challenging. Optical coherence tomography (OCT) has been recognized as a reliable tool to assess the retina as a proxy of neurodegeneration. We aimed to explore the possible role of retinal assessment in differential diagnosis between ET and early PD.

Methods:
Macular layers and peripapillary retinal nerve fiber layer (RNFL) thickness among ET, early PD, and healthy controls (HCs) were assessed using OCT.

Results:
Forty-two eyes from 23 ET, 41 eyes from 21 early PD, and 33 eyes from 17 HCs were analyzed. Macular RNFL, ganglion cell layer, inner plexiform layer, and inner nuclear layer were thinner in PD as compared with ET and even more in HCs. Differences between ET and PD were more evident when considering the TD-PD subgroup, especially for RNFL. Among ET patients, thickness of the inner macular layers showed negative linear relationship with both age at onset and disease duration. Peripapillary temporal quadrant thinning was found in ET compared with HCs.

Conclusions:
Macular inner retina was thinner in patients with ET and early PD compared with HCs. These findings suggest that the retinal assessment may have a utility in the differential diagnosis between ET and PD.
Perivascular and peribronchiolar granuloma-associated lymphoid tissue and B-cell gene expression pathways identify asymptomaticMycobacterium tuberculosislung infection in Diversity Outbred mice
Authors: Koyuncu Deniz , Tavolara Thomas , Gatti Daniel M. , Gower Adam C. , Ginese Melanie L. , Kramnik Igor , Yener Bülent , Niazi Muhammad Khalid Khan , Gurcan Metin , Alsharaydeh Anas , Beamer Gillian 
Published: July 2023

ABSTRACTHumans are highly genetically diverse, and most are resistant toMycobacterium tuberculosis.However, lung tissue from genetically resistant humans is not readily available to identify potential mechanisms of resistance. To address this, we modelM. tuberculosisinfection in Diversity Outbred mice. Like humans, Diversity Outbred mice also exhibit genetically determined susceptibility toM. tuberculosisinfection: Progressors who succumb within 60 days of a low dose aerosol infection due to acute necrotizing granulomas, and Controllers who maintain asymptomatic infection for at least 60 days, and then develop chronic pulmonary TB with occasional necrosis and cavitation, over months to greater than 1 year. Here, we identified specific regions of granuloma-associated lymphoid tissue (GrALT) and B-cell gene expression pathways as key features of asymptomatic lung infection using cytokine, antibody, granuloma image, and gene expression datasets. Cytokines and anti-M. tuberculosiscell wall antibodies discriminated acute vs chronic pulmonary TB but not asymptomatic lung infection. To find unique features of asymptomatic lung infection, we trained a weakly supervised, deep-learning neural network on lung histology images. The neural network accurately produced an interpretable imaging biomarker: perivascular and bronchiolar lymphocytic cuffs, a type of GrALT. We expected CD4 T cell genes would be highly expressed in asymptomatic lung infection. However, the significantly different, highly expressed genes in lungs of asymptomatically infected Diversity Outbred mice corresponded to B-cell activation, proliferation, and antigen-receptor signaling, includingFcrl1, Cd79, Pax5, Cr2,andMs4a1. Overall, our results suggest that genetically controlled B-cell responses are important for establishing asymptomaticM. tuberculosislung infection.
Pericardial & Mediastinal Fat-Associated Lymphoid Clusters are rapidly activated in an alkane induced model of Systemic Lupus Erythematosus
Authors: Bentkowska Karolina , Hardgrave Alex , Iqbal Nadia , Gresty Laura , Marsden Bethany , Macharia Sheila , Jackson-Jones Lucy 
Published: July 2023

AbstractSystemic Lupus Erythematosus (SLE) is an autoimmune disease predominated by auto-antibodies that recognise cellular components. Pleural involvement is the most common SLE-related lung disease. Natural antibodies are rapidly secreted by innate-like B cells following perturbation of homeostasis and are important in the early stages of immune activation. The serous cavities are home to large numbers of innate-like B cells present both within serous fluid and resident within fat-associated lymphoid clusters (FALCs). FALCs are important hubs for B-cell activation and local antibody secretion within the body cavities. Patients with SLE can develop anti-phospholipid antibodies and in rare situations develop alveolar haemorrhage. Utilising delivery of the hydrocarbon oil pristane in C57BL/6 mice as a model of SLE we identify a rapid expansion of pleural cavity B cells as early as day 3 after intra-peritoneal pristane delivery. Following pristane delivery, pericardial B1 B cells are proliferative, express the plasma-cell surface marker CD138 and secrete both innate and class switched antibodies highlighting that this cavity niche may play an unrecognised role in the initiation of lupus pleuritis.Graphical abstract
Coordination of gaze and action during high-speed steering and obstacle avoidance
Authors: Powell Nathaniel , Marshall Xavier , Diaz Gabriel J. , Fajen Brett R 
Published: July 2023

ABSTRACTWhen humans navigate through complex environments, they coordinate gaze and steering to efficiently sample the visual information needed to guide movement. Gaze and steering behavior during high-speed self-motion has been extensively studied in the context of automobile driving along a winding road. Theoretical accounts that have emerged from this work capture behavior during movement along explicit, well-defined paths over flat, obstacle-free ground surfaces. However, humans are also capable of visually guiding self-motion over uneven terrain that is cluttered with obstacles and may lack an explicit path. An extreme example of such behavior occurs during first-person view drone racing, in which pilots maneuver at high speeds through a dense forest. In this study, we explored the gaze and steering behavior of skilled drone pilots. Subjects guided a simulated quadcopter along a racecourse embedded within a forest-like virtual environment built in Unity. The environment was viewed through a head-mounted display while gaze behavior was recorded using an eye tracker. In two experiments, subjects performed the task in multiple conditions that varied in terms of the presence of obstacles (trees), waypoints (hoops to fly through), and a path to follow. We found that subjects often looked in the general direction of things that they wanted to steer toward, but gaze fell on nearby objects and surfaces more often than on the actual path or hoops. Nevertheless, subjects were able to perform the task successfully, steering at high speeds while remaining on the path, passing through hoops, and avoiding collisions. Furthermore, in conditions that contained hoops, subjects adapted how they approached the most immediate hoop in anticipation of the position (but not the orientation) of the subsequent hoop. Taken together, these findings challenge existing models of steering that assume that steering is tightly coupled to where actors look.
Biosynthesis of novel desferrioxamine derivatives requires unprecedented crosstalk between separate NRPS-independent siderophore pathways
Authors: Su Li , Souaibou Yaouba , Hôtel Laurence , Paris Cédric , Weissman Kira J. , Aigle Bertrand 
Published: July 2023

AbstractIron is essential to many biological processes, but its poor solubility in aerobic environments restricts its bioavailability. To overcome this limitation, bacteria have evolved a variety of strategies, including the production and secretion of iron-chelating siderophores. Here, we describe the discovery of four series of siderophores fromStreptomyces ambofaciensATCC23877, three of which are unprecedented. MS/MS-based molecular networking revealed that one of these series corresponds to acylated desferrioxamines (acyl-DFOs) recently identified fromS. coelicolor. The remaining sets include unprecedented tetra- and penta-hydroxamate acyl-DFO derivatives, all of which incorporate a previously undescribed building block. Stable isotope labeling and gene deletion experiments provide evidence that biosynthesis of the acyl-DFO congeners requires unprecedented crosstalk between two separate NRPS-independent siderophore (NIS) pathways in the producing organism. The new derivatives, whose biological role(s) remain to be elucidated, not only illustrate the unanticipated biosynthetic potential ofS. ambofaciens, but have interest in immuno-PET imaging applications.ImportanceIron-chelating siderophores play important roles for their bacterial producers in the environment, but they have also found application in human medicine both in iron chelation therapy to prevent iron overload, as well as in advanced imaging applications. In this study we report the discovery of three novel series of related siderophores, whose biosynthesis depends on the interplay between two NRPS-independent (NIS) pathways in the producing organismS. ambofaciens– the first example to our knowledge of such functional cross-talk. We further reveal that two of these series correspond to acyl-desferrioxamines which incorporate four or five hydroxamate units. Although the biological importance of these novel derivatives is unknown, the increased chelating capacity of these metabolites may find utility in diagnostic imaging (for instance89Zr based immuno-PET imaging) and other applications of metal chelators.