bluuebunny/crossref_metadata_2025_split huggingface dataset:
Visualizing Cancer Heterogeneity at the Molecular and Cellular Levels: Lessons from Rosetta
Authors: Goodwin Richard J.A. ,   Cancer Grand Challenges Rosetta Consortium, Marshall John F. , Poulogiannis George , Yuneva Mariia , Brindle Kevin M. , Takáts Zoltán , Sansom Owen J. , Bunch Josephine , Barry Simon T. ,   Cancer Grand Challenges Rosetta Consortium, Al-Afeef Ala , Amgheib Ala , Azarian Sara , Brochu-Williams Frederic , Burton Amy , Campbell Andrew , Cristaudo Vanina , Demircali Ali , Dexter Alex , Elia Efstathios , Fala Maria , Garrett Magali , Ghanate Avinash , Gilmore Ian , Gonzalez Ariadna , Grant Paul , Gupta Amit , Gurung Dipa , Hall Harry , Hamm Gregory , Inglese Paolo , Islam Najah , Jones Stewart , Karali Evi , Kazanc Emine , Koguna Hanifa , Kohli Manas , Koquna Hanifa , Koundouros Nikolaos , Kreuzaler Peter , Kyriazi Melina , Lall Sharanpreet Kall , Lewis David Y. , Ling Stephanie , Loizeau Xavier , Luptakova Dominika , Maneta-Stravrakaki Stefania , McGill Daniel , McGill Daniel , McKenzie James , McMahon Greg , Metodiev Martin , Mistry Jaynish , Monteiro Alvaro Perdones , Montezuma Erica , Morton Jennifer , Munteanu Catherine , Murta Teresa , Najumudeen Arafath , Nanda Chandan Seth , Nasif Ammar , Nikula Chelsea J. , Paauwe Madelon , Paiz Petra , Panina Yulia , Philip Robin , Poynter Liam , Pruski Pamela , Race Alan M. , Rao Jyotsna , Richings Jack , Savage Adele , Nanda Chandan Seth , Sharma Shreya , Soares Renata Filipe , Soloviev Dmitry , Spicer-Hadlington Amy , Sproat Caroline , Steven Rory T. , Sumpton David , Taylor Adam J. , Thomas Spencer , Thompson Daria , Tripp Aurelien , Tsalikis Thanasis , Tsyben Anastasia , Turseven Seyma , Vande-Voorde Johan , Vorng Jean-Luc , White Emma , Wright Alan , Wu Vincen , Xiang Yuchen , Yan Bin , Zao Jinshi , Zeiger Lucas , Zhang Junting , Zhou Weiwei 
Published: January 2025

Summary:
Understanding tumor heterogeneity is a major challenge that was recognized as one of the first Cancer Grand Challenges, with a call to provide solutions to visualize tumor heterogeneity. The Rosetta team took on this challenge, exploiting advances in spatial-omics approaches centered around mass spectrometry imaging to map tumor heterogeneity at the cellular and molecular scales with different levels of resolution.
See related article by Bressan et al., p. 16
See related article by Stratton et al., p. 22
See related article by Bhattacharjee et al., p. 28
Extended Reconstructed Sea Surface Temperature, Version 6 (ERSSTv6). Part II: Upgrades on Quality Control and Large-Scale Filter
Authors: Huang Boyin , Yin Xungang , Boyer Tim , Liu Chunying , Menne Matthew , Rao Yuhan Douglas , Smith Thomas , Vose Russell , Zhang Huai-Min 
Published: January 2025

Abstract
NOAA’s Extended Reconstructed Sea Surface Temperature (ERSST) is a monthly 2°SST product starting from 1850. Our Part I study indicated that the performance scores of spatial correlation coefficient (SCC) and root-mean-square difference (RMSD) dropped clearly after the mid-1970s in the analysis of ERSST with an artificial neural network (ANN) method. In this Part II study, we demonstrate that ERSST with the ANN method can further be improved progressively in the final ERSSTv6 by the following steps: 1) applying a nearest neighbor check (NNC) quality-control algorithm on ship observations, 2) applying a large-scale (>200 km) filter (LS200) on SST superobservations, and 3) upgrading algorithms in proxy SST from ice concentration. These progressive improvements were assessed against validation and observation datasets. In comparison with ERSST with the ANN method alone, the quality of ERSSTv6 improves in the statistical metrics of SCC and RMSD by 2%–11% and 0.01°–0.24°C, respectively, in the global oceans. In the ice-covered regions, SST bias and RMSD decrease by 0.67° and 0.29°C, respectively.

Significance Statement
The ERSSTv6 using an artificial neural network (ANN) is further improved by progressively implementing the nearest neighbor check (NNC) in quality control (QC) on ship observations, large-scale filter (LS200) on SST superobservations, and ice-SST proxy algorithm in the ice-covered regions.
Inhibitory Effects of Different Doses of Matrine on Breast Cancer and Its Relationship with miR-188
Authors: Liu Zhao , Xu Jian , Xia Heng , Wang Miaofeng , Xu Qianlan 
Published: January 2025

Background Malignant tumors represented by breast cancer still have limitations in clinical treatment methods. Matrine is a compound with anticancer activity, but its inhibitory effect on breast cancer and its mechanism for regulating miR-188 are still unclear. Purpose Therefore, it is necessary to explore more effective breast cancer treatment methods and understand the intervention mechanism of matrine and its effect on miR-188. Methods A nude mouse model of breast cancer was in this study constructed and divided into a model group, 4 mg/kg group, 8 mg/kg group, drug group, and miR-188 mimic group. Different concentrations of matrine were administered intragastrically, and cell proliferation and apoptosis were observed. At the same time, a specific mechanism for miR-188 in the anti-breast cancer effect of matrine was explored by overexpressing miR-188. Results It was found that matrine inhibited the proliferation of breast cancer cells and caused cancer cell apoptosis. In addition, it inhibited cell migration in a dose-dependent manner. At the same time, miR-188 was lowly expressed in breast cancer and significantly increased after matrine treatment. Overexpression of miR-188 enhanced the antitumor effect of matrine. Phosphatase and tensin homolog (PTEN) was also activated in the surface by matrine, and expressions of matrix metalloproteinase-9 (MMP-9) and matrix metalloproteinase-2 (MMP-2) were inhibited. This study shows that matrine has a significant inhibitory effect on breast cancer, and its mechanism is related to upregulation of miR-188, activation of PTEN, and inhibition of MMP-9 and MMP-2 expressions. Conclusion miR-188 is involved in the anti-breast cancer process, which can be used as the theoretical basis of matrine potential drugs for breast cancer treatment. Meanwhile, miR-188 and matrine will also have great potential in developing new breast cancer treatment methods, which can provide ideas and a basis for further development of related studies.
Scorpionism in the State of Bahia, Brazil: A More Neglected Problem During the Pandemic?
Authors: Braga Jacqueline Ramos Machado , dos Santos Luís Vinícius Sena , Mata Amanda Cristina de Souza , Moraes Isabel Cristina 
Published: January 2025

Introduction During the confinement in the COVID-19 pandemic, there was an increase in the production of urban waste, increasing the risk of accidents caused by scorpions. We sought to determine the clinical and epidemiologic aspects of scorpionism records in the 7 mesoregions of the State of Bahia, Brazil, examining differences in periods before and during the pandemic. Methods Data were obtained from SINAN, Brazil's Notifiable Diseases Information System (January 2010–December 2021). Measures of morbidity (incidence and lethality) and mortality were evaluated, comparing the averages before (2018 and 2019) and during the pandemic (2020 and 2021). Sociodemographic data (ie, gender, age group, and race) and conditions of care (ie, time, classification, and evolution) were compared by mesoregion. Results A total of 159,982 cases of scorpionism were registered, with a higher proportion in the prepandemic period ( P<0.05) and a higher average incidence in the Center South of Bahia (267.32; n=82,059; 51.29%). Most cases occurred in mixed-race individuals (61.73%), females (n=81,292; 51.22%), and individuals between 20 and 39 y of age (n=48,876; 30.55%) and most cases were classified as mild (n=136,816; 85.52%), were attended in <1 h (n=81,941; 51.22%), and progresses to cure (n=142,082; 88.81%). There were 263 deaths (0.16%), with a mortality rate of 1.77/100,000 inhabitants and a lethality of 0.16%. Conclusion It is suggested that during the COVID-19 pandemic, despite the upward curve of accidents, social isolation measures and collective fear may have decreased the search for medical care after a scorpion sting, reducing notifications of the condition in the State of Bahia.
Clinical validation of artificial intelligence-based single-subject morphometry without normative reference database
Authors: Hedderich Dennis M , Opfer Roland , Krüger Julia , Spies Lothar , Yakushev Igor , Buchert Ralph 
Published: January 2025

Background Single-subject voxel-based morphometry (VBM) is a powerful technique for reader-independent detection of brain atrophy in structural magnetic resonance imaging (MRI) to support the (differential) diagnosis and staging of neurodegenerative diseases in individual patients. However, VBM is sensitive to the MRI scanner platform and details of the acquisition sequence. To mitigate this limitation, we recently proposed and technically validated a convolutional neural network (CNN)-based VBM which does not rely on a normative reference database. Objective Clinical validation of CNN-based VBM. Methods CNN-based VBM was compared with conventional VBM based on a mixed-scanner normative database in 227 consecutive patients (66.0 ± 9.6 years, 53.3% female) with suspected dementing neurodegenerative disease. VBM maps were interpreted visually by two experienced readers, first with respect to the presence of any neurodegenerative disease, then for the differentiation between Alzheimer's disease (AD)-typical and non-AD atrophy patterns. A Likert 6-score was used for both tasks. Simultaneously acquired positron emission tomography (PET) with 18F-fluorodeoxyglucose (FDG) served as reference standard. Results Repeated-measures ANOVA revealed a significant impact of the VBM method on the visual detection of any neurodegenerative disease (p < 0.001). Balanced accuracy/sensitivity/specificity were 80.4/86.3/74.5% for CNN-based VBM versus 75.7/79.5/71.8% for conventional VBM. Differentiation between AD and non-AD typical atrophy patterns did not differ between both VBM methods (p = 0.871). Conclusions CNN-based VBM provides clinically useful accuracy for the detection of neurodegeneration-suspect atrophy with higher sensitivity than conventional VBM with a mixed-scanner normative reference database and without compromising specificity.
A Bleed From the Distal Anastomosis Site of a Bypass Graft From the Left Common Iliac Artery to the Celiac Trunk Treated With a Surgeon-Modified Fenestrated Endograft
Authors: Grima Matthew Joe , Zammit Michaela , Cassar Kevin 
Published: January 2025

Purpose: The use of surgeon-modified fenestrated endograft to treat a bleeding complication in the common iliac artery. Technique: An Endurant limb graft was modified on back table in theater after planning the fenestration using a semi-automated centerline. The Endurant stent was planned to land flush at the aortic bifurcation. The Endurant limb was partially deployed until the fenestration opened facing the anastomosis to enable rotation and axial movement of the stent graft, if needed. By obtaining access from the contralateral common femoral artery, the fenestration was cannulated using up-and-over technique and bypass graft cannulated. The Endurant stent graft was then fully deployed. Through a separate puncture in the ipsilateral sheath, the fenestration was re-cannulated using a hydrophilic wire, and a balloon-expandable covered stent was introduced through a 7F sheath and then flared in a kissing fashion along with the Endurant stent. The distal part of the Endurant limb was extended with a bare metal stent into the external iliac artery. Control angiography was satisfactory, and bleeding was controlled. Conclusion: Surgeon-modified stent graft in the iliac arteries can be used even in the context of non-aneurysmal disease to treat patients in high-risk emergency situations and could be part of the armamentarium of the vascular surgeon. Clinical Impact Physician-modified endografts are increasingly being used by vascular surgeons and interventional radiologists to treat patients with complex aorto-iliac aneurysms in whom custom-made endograft provided by the manufacturer is too expensive to produce and/or patient requires urgent surgery. Data on the use of physician-modified endografts to treat non-aneurysmal disease is lacking. This technical note highlights the use of physician-modified endograft to treat a patient with bleeding complication from the distal anastomosis of an iliac artery to coeliac trunk bypass graft in whom open surgery posed unacceptable high morbidity and mortality risks. This technical note continues to add more information and knowledge to the vascular surgery / interventional radiology communities on the use of physician-modified endografts and also adds another tool in the armamentarium of teams involved.
Optimizing haemodialysis outcomes: A pilot study on the effectiveness of multidisciplinary clinics in enhancing vascular access and improving patient outcomes
Authors: Lai Boon Cheok , Tay Hui Boon , Lee Pei Shan 
Published: January 2025

Background Patients with kidney failure face increased health risks during early haemodialysis due to physical and emotional challenges. Most patients begin haemodialysis using catheters, which increases mortality and complication risks. This study evaluates the impact of multidisciplinary clinics on vascular access and patient outcomes on haemodialysis. Objectives To compare the influence of multidisciplinary clinics with conventional clinics on the utilisation of definitive vascular access for haemodialysis. Methods This is a single-centre, retrospective cohort study included 145 patients newly started on haemodialysis. Patients were evaluated over a 12-month follow-up period (July 2018 to December 2020). The primary outcome was definitive vascular access for haemodialysis. Secondary outcomes included the frequencies of catheter-related bloodstream infections, intensive care unit admissions, and recurrent hospitalizations. Results The study analysed 53 and 92 participants in the multidisciplinary clinics and conventional groups, respectively. Although only 10% of patients started haemodialysis with definitive access, the multidisciplinary clinics group exhibited significantly higher utilisation of definitive vascular access after 6 months (OR = 2.44, 95% CI 1.16-5.26, p = .039). The multidisciplinary clinics group had lower risks of intensive care unit admission (OR 0.92, 95% CI 1.03-1.15, p = .026) and recurrent hospitalization (OR 0.13, 95% CI 2.06-26.68, p = .001). Although not statistically significant (OR 0.34, 95% CI 0.47-18.46, p = .709), the multidisciplinary clinics group had a lower incidence of catheter-related infections. Conclusions Multidisciplinary care significantly increases the use of definitive vascular access, reduces intensive care unit admissions, and hospitalization rates, demonstrating the effectiveness of comprehensive, coordinated care in managing patients on haemodialysis.
A Critical Appraisal of the Application of Frailty and Sarcopenia in the Spinal Oncology Population
Authors: MacLean Mark A. , Charles Antoinette J. , Georgiopoulos Miltiadis , Phinney Jackie , Charest-Morin Raphaële , Goodwin Rory , Laufer Ilya , Fehlings Michael G. , Shin John , Dea Nicholas , Rhines Laurence D. , Sahgal Arjun , Gokaslan Ziya , Stephens Byron , Disch Alexander C. , Kumar Naresh , O’Toole John , Sciubba Daniel M. , Netzer Cordula , Goldschlager Tony , Gibbs Wende , Weber Michael H. ,   on behalf of the AO Spine Knowledge Forum Tumor
Published: January 2025

Study Design Systematic review and clinimetric analysis. Objectives Frailty and sarcopenia predict worse surgical outcomes among spinal degenerative and deformity-related populations; this association is less clear in the context of spinal oncology. Here, we sought to identify frailty and sarcopenia tools applied in spinal oncology and appraise their clinimetric properties. Methods A systematic review was conducted from January 1st, 2000, until June 2022. Study characteristics, frailty tools, and measures of sarcopenia were recorded. Component domains, individual items, cut-off values, and measurement techniques were collected. Clinimetric assessment was performed according to Consensus-based Standards for Health Measurement Instruments. Results Twenty-two studies were included (42 514 patients). Seventeen studies utilized 6 frailty tools; the three most employed were the Metastatic Spine tumor Frailty Index (MSTFI), Modified Frailty Index-11 (mFI-11), and the mFI-5. Eight studies utilized measures of sarcopenia; the three most common were the L3-Total Psoas Area (TPA)/Vertebral Body Area (VBA), L3-TPA/Height2, and L3-Spinal Muscle Index (L3-Cross-Sectional Muscle Area/Height2). Frailty and sarcopenia measures lacked or had uncertain content and construct validity. Frailty measures were objective except the Johns-Hopkins Adjusted Clinical Groups. All tools were feasible except the Hospital Frailty Risk Score (HFRS). Positive predictive validity was observed for the HFRS and in select studies employing the mFI-5, MSTFI, and L3-TPA/VBA. All frailty tools had floor or ceiling effects. Conclusions Existing tools for evaluating frailty and sarcopenia among patients undergoing surgery for spinal tumors have poor clinimetric properties. Here, we provide a pragmatic approach to utilizing existing frailty and sarcopenia tools, until more clinimetrically robust instruments are developed.
What is the Optimal Management of Metastatic Spine Patients With Intermediate Spinal Instability Neoplastic Scores: To Operate or Not to Operate?
Authors: Kwan William Chu , Zuckerman Scott L. , Fisher Charles G. , Laufer Ilya , Chou Dean , O'Toole John E. , Schultheiss Markus , Weber Michael H. , Sciubba Daniel M. , Pahuta Markian , Shin John H. , Fehlings Michael G. , Versteeg Anne , Goodwin Matthew L. , Boriani Stefano , Bettegowda Chetan , Lazary Aron , Gasbarrini Alessandro , Reynolds Jeremy J. , Verlaan Jorrit-Jan , Sahgal Arjun , Gokaslan Ziya L. , Rhines Laurence D. , Dea Nicolas 
Published: January 2025

Study Design Systematic review. Objective In patients with extradural metastatic spine disease, we sought to systematically review the outcomes and complications of patients with intermediate Spinal Instability Neoplastic Score (SINS) lesions undergoing radiation therapy, percutaneous interventions, minimally invasive surgeries, or open spinal surgeries. Methods Following PRISMA guidelines for systematic reviews, MEDLINE, EMBASE, Web of Science, the Cochrane Database of Systematic Reviews and the Cochrane Center Register of Controlled Trials were queried for studies that reported on SINS intermediate patients who underwent: 1) radiotherapy, 2) percutaneous intervention, 3) minimally invasive, or 4) open surgery. Dates of publication were between 2013-22. Patients with low- or high-grade SINS were excluded. Outcome measures were pain score, functional status, neurological outcome, ambulation, survival, and perioperative complications. Results Thirty-nine studies (n = 4554) were included that analyzed outcomes in the SINS intermediate cohort. Radiotherapy appeared to provide temporary improvement in pain score; however, recurrent pain led to surgery in 15%–20% of patients. Percutaneous vertebral augmentation provided improvement in pain. Minimally invasive surgery and open surgery offered improvement in pain, quality of life, neurological, and ambulatory outcomes. Open surgery may be associated with more complications. There was limited evidence for radiofrequency ablation. Conclusion In the SINS intermediate group, radiotherapy was associated with temporary improvement of pain but may require subsequent surgery. Both minimally invasive surgery and open spinal surgery achieved improvements in pain, quality of life, and neurological outcomes for patients with spine metastases. Open surgery may be associated with more complications.
Risk Factors for the Development of Neurological Deficits in Metastatic Spinal Disease: An International, Multicenter Delphi Study
Authors: Huele Eline H. , Gal Roxanne , Eppinga Wietse S.C. , Verkooijen Helena M. , O’Toole John E. , Laufer Ilya , Sciubba Daniel M. , Netzer Cordula , Foppen Wouter , Sahgal Arjun , Fehlings Michael G. , Lo Sheng-fu L. , Fisher Charles G. , Rhines Laurence D. , Reynolds Jeremy J. , Lazary Aron , Gasbarrini Alessandro , Dea Nicolas , Weber Michael H. , Verlaan Jorrit Jan 
Published: January 2025

Study Design Delphi study Objective The objective of this study was to identify risk factors associated with the development and/or progression of neurological deficits in patients with metastatic spinal disease. Methods A three-round Delphi study was conducted between January-May 2023 including AO Spine members, comprising mainly neurosurgeons and orthopedic surgeons. In round 1, participants listed radiological factors, patient characteristics, tumor characteristics, previous cancer-related treatment factors and additional factors. In round 2, participants ranked the factors on importance per category and selected a top 9 from all factors. Kendall’s W coefficient of concordance was calculated as a measure of consensus. In the final round, participants provided feedback on the rankings resulting from round 2. Lastly, the highest-ranking factors were more clearly defined and operationalized by an expert panel. Results Over two hundred physicians and researchers participated in each round. The factors listed in the first round were collapsed into 12 radiological factors, 14 patient characteristics, 6 tumor characteristics and 12 previous cancer-related treatment factors. High agreement was found in round 3 on the top-half lists in each category and the overall top 9, originating from round 2. Kendall’s W indicated strong agreement between the participants. ‘Epidural spinal cord compression’, ‘aggressive tumor behavior’ and ‘mechanical instability’ were deemed most influential for the development of neurological deficits. Conclusion This study provides factors that may be related to the development and/or progression of neurological deficits in patients with metastatic spinal disease. This list can serve as a basis for future directions in prognostication research.
Single-session gamma sensory stimulation entrains real-time electroencephalography but does not enhance perception, attention, short-term memory, or long-term memory
Authors: Wu Cai-Syuan , Lin Ting-Xuan , Lo Yu-Hui , Ke Shih-Chiang , Sahu Prangya Parimita , Tseng Philip 
Published: January 2025

Background Studies have shown that gamma (40 Hz) audiovisual stimulation can enhance gamma oscillations and improve cognitive functioning in patients with Alzheimer's disease. However, despite promising long-term results, the efficacy of short-duration or single-session 40 Hz entrainment in humans has been questioned by behavioral studies that fail to find observable cognitive aftereffects, for two possible reasons: 1) lack of validated gamma entrainment, as most studies lacked concurrent electroencephalography (EEG) data to verify that gamma neural entrainment did take place, and 2) lack of diverse cognitive tests, as most studies did not test a wide range of cognitive factors. Objective This study aimed to increase sensitivity for detecting single-session gamma entrainment. We employed 1) mid- and post-stimulation EEG monitoring to ensure entrainment worked, and 2) a comprehensive cognitive battery that probes perception, attention, working memory, and long-term memory. Methods Participants received 30 min of synchronized 40 Hz light and sound stimulation, followed by a visual perceptual task, attentional network task, change detection working memory task, and long-term picture memory task, with concurrent EEG. Results We observed robust 40 Hz EEG entrainment during stimulation but no significant 40 Hz oscillation after stimulation, and no significant cognitive improvements. Conclusions Despite robust 40 Hz online entrainment, gamma sensory entrainment requires consistent long-term exposure to induce cognitive and neurological changes. Future research should determine the optimal duration and frequency of 40 Hz stimulation to maximize its therapeutic potential.