bluuebunny/crossref_metadata_2025_split huggingface dataset:
The expression and distribution of TACAN in human and rat bladders
Authors: Lu Qudong , Liu Qian , Chen Shiwei , Wang Jiaolian , Chen Yongjie , Sun Bishao , Yang Zhenxing , Feng Huan , Yi Shanhong , Chen Wei , Zhu Jingzhen 
Published: August 2023

AbstractObjectivesA lot of ion channels participate in the regulation of bladder function. TACAN, a new mechanosensitive ion channel, was first discovered in 2020. TACAN has been found to be expressed in many tissues, such as the dorsal root ganglia (DRG) and adipose tissue. However, it is unclear whether or not TACAN is expressed in the bladder. In this work, we decided to study the expression and distribution of TACAN in human and rat bladders. Meanwhile, the expression of TACAN in the rat model of interstitial cystitis/bladder pain syndrome (IC/BPS) was studied.MethodsHuman bladder tissues were obtained from female patients. Cyclophosphamide (CYP) was used to build the rat model of IC/BPS. Real‐time polymerase chain reaction, agarose gel electrophoresis, and western blotting were used to assess the expression of TACAN in human and rat bladders. Immunohistochemistry and immunofluorescence were used to observe the distribution of TACAN in human and rat bladders. Hematoxylin‐eosin stain, withdrawal threshold, and micturition interval were used to evaluate animal models.ResultsThe results of agarose gel electrophoresis and western blotting suggested that TACAN was expressed in human and rat bladders. Immunohistochemical results suggested that TACAN showed positive immunoreaction in the urothelial and detrusor layers. The immunofluorescence results indicated that TACAN was co‐stained with UPKIII, α‐SMA, and PGP9.5. The IC/BPS model was successfully established with CYP. The mRNA and protein expression of TACAN was upregulated in the CYP‐induced rat model of IC/BPS.ConclusionsTACAN was found in human and rat bladders. TACAN was mainly distributed in the urothelial and detrusor layers and bladder nerves. The expression of TACAN was upregulated in the CYP‐induced rat model of IC/BPS. This new discovery will provide a theoretical basis for future research on the function of TACAN in the bladder and a potential therapeutic target for IC/BPS.
Temperature‐induced lipocalin‐mediated membrane integrity: Possible implications for vindoline accumulation in Catharanthus roseus leaves
Authors: Mall Maneesha , Shanker Karuna , Nagegowda Dinesh A. , Samad Abdul , Kalra Alok , Pandey Alok , Sundaresan Velusamy , Shukla Ashutosh K. 
Published: August 2023

AbstractPlant lipocalins perform diverse functions. Recently, allene oxide cyclase, a lipocalin family member, has been shown to co‐express with vindoline pathway genes in Catharanthus roseus under various biotic/abiotic stresses. This brought focus to another family member, a temperature‐induced lipocalin (CrTIL), which was selected for full‐length cloning, tissue‐specific expression profiling, in silico characterization, and upstream genomic region analysis for cis‐regulatory elements. Stress‐mediated variations in CrTIL expression were reflected as disturbances in cell membrane integrity, assayed through measurement of electrolyte leakage and lipid peroxidation product, MDA, which implicated the role of CrTIL in maintaining cell membrane integrity. For ascertaining the function of CrTIL in maintaining membrane stability and elucidating the relationship between CrTIL expression and vindoline content, if any, a direct approach was adopted, whereby CrTIL was transiently silenced and overexpressed in C. roseus. CrTIL silencing and overexpression confirmed its role in the maintenance of membrane integrity and indicated an inverse relationship of its expression with vindoline content. GFP fusion‐based subcellular localization indicated membrane localization of CrTIL, which was in agreement with its role in maintaining membrane integrity. Altogether, the role of CrTIL in maintaining membrane structure has possible implications for the intracellular sequestration, storage, and viability of vindoline.
Staff‐rated ability to provide post‐diagnosis support for patients with mild cognitive impairment and related factors
Authors: Iiyama Yuki , Kaba Yuzaburo , Kusaba Tomoko , Furumura Mitsuyo 
Published: August 2023

AbstractBackgroundProactive interventions for patients with mild cognitive impairment (MCI) are required. We aimed to determine the staff‐rated ability to provide post‐diagnosis support for patients with MCI at a medical centre for dementia and the related factors.MethodsWe conducted a web‐based survey on post‐diagnosis support for patients with MCI among healthcare personnel, such as mental health social workers and public health nurses (hereafter referred to as ‘staff’), in consultation and support roles at medical centres for dementia nationwide. The latent characteristic value for ‘staff's self‐rated ability to provide post‐diagnosis support to patients with MCI’, which was estimated using the one‐parameter logistic model of item response theory, was used as the dependent variable. Multivariate linear regression analysis was used to examine the factors associated with the dependent variable.ResultsWe conducted the study at 482 medical centres for dementia. We received responses from 162 participants, 158 of which were valid. We applied item response theory to 45 staff‐rated items regarding post‐diagnosis support for patients with MCI and found that item difficulty ranged from −2.56 to 1.02; 40 items had negative values and were deemed relatively easy. The staff‐rated ability to provide post‐diagnosis support was significantly higher for ‘The role in assisting patients with MCI is clear’ (P < 0.005), ‘A reasonable number of personnel is available to assist immediately after MCI diagnosis’ (P = 0.001), and ‘Collaboration with family physician available immediately after MCI diagnosis’ (P < 0.001).ConclusionsThe results of this study showed that staff rated their ability to provide post‐diagnosis support for patients with MCI as relatively easy. The staff‐rated ability to provide post‐diagnosis support to patients with MCI may be enhanced by increased availability of staff immediately after MCI diagnosis, clarification of staff roles, and collaboration with family doctors.
Frame alignment processes for locally useful agricultural soil research and extension: The role of farm advisors
Authors: Higgins Vaughan , Bryant Melanie , Allan Catherine , Cockfield Geoff , Leith Peat , Cooke Penny 
Published: August 2023

AbstractFarm advisors are recognised as playing an increasingly central role in facilitating interactions between scientists and farmers to improve local implementation of sustainable soil management practices and agricultural innovations more broadly. However, there has been limited scrutiny of what farm advisors do when faced with conflicting interpretations among actors over techniques or approaches for facilitating agricultural innovation. This article advances knowledge in this area by investigating the role of farm advisors in aligning different frames on agricultural soil research and extension across seven Australian mixed farming regions. Drawing upon theoretical work on frame alignment, we argue that farm advisors use three types of strategies to align conflicting frames—frame bridging, frame amplification and frame transformation. These strategies seek to frame local soil research and extension priorities in ways that are assumed to resonate more closely with the frames of multiple constituents, such as farmers and soil scientists. Through our analysis, we argue that the application of a frame alignment approach enables greater precision in identifying which (a) interactive and social learning processes, (b) key local influencers and communities of practice and (c) resourcing and governance arrangements are most likely to be effective in facilitating soil research and extension that is locally useful and useable.
What students say to be ready for acute care: Implications for preceptors
Authors: Oldenburg Hannah , Hake Melissa , Rindflesch Aaron 
Published: August 2023

AbstractBackgroundStudent readiness for clinical education experiences is an important variable for clinical preceptors. Readiness has been reported from the viewpoints of clinicians, academic faculty, clinical preceptors, employers and novice graduate physical therapists. Perspectives from physical and occupational therapy students could better prepare preceptors in the acute care setting.ApproachThematic analysis was conducted following the approach described by Braun and Clarke. The purpose of this study was to describe physical and occupational therapy student perceptions of readiness for full‐time acute care clinical education experiences. Twenty‐one physical and occupational therapy students in process of completing full‐time clinical education experiences at a large academic medical centre were qualitatively interviewed in small groups. A two‐stage iterative process of thematic inquiry was used to induce themes about student readiness for full‐time acute care clinical experiences. Transcription, coding and thematic analysis were completed. Students also completed a one‐time questionnaire with demographic and learner characteristic scales.FindingsParticipants were on average 26.4 years old, predominantly female and White, and had completed one full‐time clinical education experience prior to participation. Four major thematic categories of student readiness for acute care clinical experiences emerged: (1) mindset and willingness; (2) knowledge and experience; (3) communication and collaboration; and (4) planning and prioritising.ImplicationsStudents report their readiness is facilitated by preceptors who can model flexibility, foster communication that promotes clinical reasoning, implement an orientation to the acute care culture and learning resources and scaffold roles and responsibilities to aid professional authority.
Feasibility and safety of Post-Urgent PCI Same-DaY discharge (PUSDY Trial) in China: protocol for a multicentre randomised controlled trial
Authors: Li Xiang , Zhang Feng , Yang Duo , Ye Ming , Zu Xiaolin , Zhai Guangyao , Fu Hao , Qu Chao , Jin Yanyan , Zhou Can , Qi Shuying , Wang Chenggang , Gao Hai 
Published: August 2023

IntroductionIn the Chinese healthcare system, where there is overcrowding in hospitals, especially in tertiary care centres, adoption of same-day discharge (SDD) post-percutaneous coronary intervention (PCI) could potentially lead to significant savings of healthcare resources and costs. This study is a non-inferiority trial examining whether post-PCI SDD is feasible in China. The primary hypothesis is that patient outcomes in post-urgent PCI SDD patients are non-inferior to regular discharge patients.Methods and analysisPost-Urgent PCI Same-DaY is an investigator-initiated multicentre randomised unblinded clinical non-inferiority trial, with 1:1 centralised randomisation to the SDD or usual care (UC) group. Based on sample size calculations, 1296 patients from at least three hospitals, with mild to moderate myocardial infarction, will be included, and acute coronary syndrome patients will be excluded. All patients will receive UC while patients assigned to the SDD group will be discharged on the same day or within 12 hours post-PCI. The primary outcome is major adverse cardiovascular and cerebrovascular events 30 days after discharge. The secondary outcomes are all-cause mortality, bleeding and access site complications. The outcome rates will be compared between groups with the absolute risk difference with a 95% CI.Ethics and disseminationThe study protocol V.2.0 has been approved on 21 January 2022 by the Ethics Committee of Beijing Anzhen Hospital, Capital Medical University (approval number: 2021 KLSD No. 23). The outcomes of this study will be disseminated through a peer-reviewed journal and presented at international conferences.Trial registration numberChiCTR 2200057065; China Clinical Trial Registration.
Clinical Characteristics and Risk Factors for Allergic Rhinitis in Children with Epistaxis
Authors: Qing Jing , Cai Yili , Tang Shixiong , Wang Yaowen 
Published: August 2023

Background. Epistaxis is frequently observed in children with allergic rhinitis. However, few studies have addressed the clinical characteristics and risk factors for allergic rhinitis in children with epistaxis. This study aimed to describe the factors associated with allergic rhinitis in children with epistaxis. Methods. In total, we recruited 80 children (aged 3–14 years) who presented with epistaxis at a tertiary hospital between January 2014 and January 2022. The follow-up duration was at least 3 months, and we performed a multivariate logistic regression analysis to identify the risk factors for allergic rhinitis. Results. Among the 80 children examined, 57 (71.25%) had allergic rhinitis. Epistaxis mainly occurred in autumn in children with allergic rhinitis; in contrast, it mostly occurred in summer in children without it (P = 0.029). Mites are common allergens for allergic rhinitis in children with epistaxis; the univariate analysis revealed significant differences between allergic-rhinitis group and nonallergic-rhinitis group in the number of allergens

P

<

0.001

and total IgE

P

<

0.001

. The difference in severity of nasal symptoms between the two groups was statistically significant and included nasal obstruction

P

<

0.001

, rhinorrhea

P

<

0.001

, sneezing

P

<

0.001

, and nasal itching

P

<

0.001

. After adjusting for potential confounders, the severity of rhinorrhea symptoms was found to be associated with an increased risk of allergic rhinitis in children with epistaxis (odds ratio: 3.86; 95% confidence interval: 1.61–9.26; P = 0.003). Conclusions. Observing the onset season, number of allergens, total IgE, and nasal symptoms in cases of epistaxis could suggest the presence of associated allergic rhinitis and reduce the number of missed diagnoses; antiallergic drugs could help control epistaxis in these cases.
PENGETAHUAN, SIKAP, DAN PERILAKU PASIEN GLAUKOMA DI PMN RS MATA CICENDO TERHADAP PENYAKIT GLAUKOMA
Authors: Akbari Anamika Lingga Anjani , Rifada R. Maula , Gustianty Elsa 
Published: August 2023

Pendahuluan: Glaukoma merupakan penyebab kedua kebutaan terbanyak secara global. Sebagian besar kasus glaukoma bersifat asimptomatik, sehingga pasien tidak sadar dengan penyakitnya sampai munculnya gangguan penglihatan hingga kebutaan. Melalui pengetahuan, sikap, dan perilaku (PSP) pasien yang baik dapat mendukung pengendalian keparahan penyakit glaukoma. Akan tetapi, di Indonesia penelitian yang menunjukkan tingkat PSP pasien glaukoma terhadap penyakit glaukoma masih tergolong sedikit. Oleh karena itu, penelitian ini bertujuan untuk menilai tingkat PSP pasien glaukoma di PMN RS Mata Cicendo terhadap penyakit glaukoma.
Metode: Studi ini menggunakan metode kuantitatif dengan desain penelitian deskriptif cross-sectional melalui pengisian kuesioner pada 59 pasien glaukoma di PMN RS Mata Cicendo pada bulan Februari 2023. Pada penelitian ini terdapat tiga variabel yang dinilai, yaitu pengetahuan, sikap, dan perilaku.
Hasil: Pasien glaukoma menunjukkan pengetahuan baik (35,6%), cukup (59,5%), dan kurang (5,1%) dengan hanya 42,4% pasien yang bersikap positif dan 49,2% pasien berperilaku baik. Secara keseluruhan tingkat PSP pasien adalah 47%. PSP yang baik banyak ditemukan pada pasien dengan durasi pengobatan ≥6 bulan.
Simpulan: Tingkat PSP pasien glaukoma terhadap penyakit glaukoma yang sudah cukup baik tetap harus ditingkatkan dengan strategi edukasi agar tingkat sikap dan perilaku pasien jauh lebih baik.
Contesting the Shifting Center of World Christianity to Africa: Methodological Reconsiderations
Authors: Ilo Stan Chu 
Published: August 2023

Abstract
This essay contests the regnant narratives that the center of gravity of Christianity is shifting to Africa. First, it is not always clear what theorists mean as to what constitutes the centering of Christianity and what ‘shifting centers’ mean beyond the claims of the demographic, social and cultural history of Christian expansion. Furthermore, the field of world Christianity and the methods for studying it continue to evolve, pointing more to a divergence than a convergence in both method and subject matter. Through critical study of old and emerging methodologies, the essay proposes that the claims about world Christianity and its variants and features in Africa must be rethought in ways that give value to the actual faith and context of Africans. The essay proposes four possible approaches for developing an account of Christianity in Africa and the world, focusing on theological, missional, and biblical prescriptive perspectives, that complement the predominant descriptive, sociological, and historical accounts of Christian expansion in Africa in current scholarship.
Prophetic Dialogue: Ecclesiology for a Vital Church
Authors: Otu Idara 
Published: August 2023

Abstract
The Catholic church is a community of faith, and an agency of social transformation for a distressed African continent. Many Africans look up to the church for renewal and direction in their quest for integral development and cosmic flourishing. This expectation is gradually weaning in the face of the multidimensional challenges that weigh on Africa. Particularly, the impoverished social order and dysfunctional political systems deprive Africans of dignified lives and sustainable development. This article builds on prophetic dialogue as an ecclesiological paradigm for local churches in engaging the African social context in a manner that inspires social transformation. Grounded on prophetic dialogue, the article proposes pathways of local churches in addressing the concrete social issues and concerns of the people in their locality. These include social discernment, social palaver, and self-emptying. Drawing insights from the teachings of Pope Francis, the essay argues that these pathways offer credible and effective channels for a transformative encounter between the Catholic Church and African society.
Cardiac magnetic resonance imaging for myocardial viability assessment: Optimizing surgical revascularization in ischemic heart disease
Authors: Sayyed Azhar , Das Subhajit , Das Patralekha , Shales Sufina , Kapoor Lalit , Saha Atanu , Narayan Pradeep 
Published: August 2023

Background Patients with poor ejection fraction undergoing coronary artery bypass grafting carry higher operative risk and have poor long-term survival. Cardiac magnetic resonance is a useful modality to assess viability which can identify patients likely to benefit most from revascularization. In this study, we aimed to assess the outcome in patients selected for surgical revascularization by cardiac magnetic resonance imaging and identify predictors associated with poor outcomes. Methods The study included patients with severely impaired left ventricular function but with at least six viable segments. Patients requiring emergency surgery, undergoing combined procedures, or where cardiopulmonary bypass was required were excluded. Cardiac magnetic resonance was carried out both preoperatively and at six months postoperatively by the same radiologist in all cases. Late gadolinium enhancement was used for the evaluation of myocardial viability. Results Amongst a total of 493 segments studied, there were 89 (18.1%) non-viable, 117 (23.7%) hibernating and 287 (58.2%) viable segments. At six months, the number of non-viable segments changed from 89 (18.1%) to 97 (19.7%), with an increase in viable segments from 287 (58.2%) to 374 (75.8%) and a corresponding reduction of hibernating segments from 117 (23.7%) to 22 (4.5%). There was improvement in ejection fraction from 28 ± 5.54 to 37 ± 5.86 ( p < 0.0001) in the entire cohort at six months. Overall mortality was 1 (3.2%). Preoperative left ventricular end-systolic volume had the strongest negative correlation with post-operative ejection fraction. Conclusion Cardiac magnetic resonance aided revascularization is associated with low mortality. Preoperative left ventricular end-systolic volume is an important determinant of postoperative ejection fraction.
Harassment in the headache field: a global web-based cross-sectional survey
Authors: de Boer Irene , Ambrosini Anna , Halker Singh Rashmi B. , Baykan Betül , Buse Dawn C. , Tassorelli Cristina , Jensen Rigmor H. , Pozo-Rosich Patricia , Terwindt Gisela M. ,   on behalf of the International Headache Society Women’s Leadership Forum
Published: August 2023

Background Matters of workplace harassment are an important issue. This issue needs to be recognized and studied to prevent occurrences. These important sensitive areas of effective workplace management are increasingly gaining more interest. We aimed to identify the prevalence of workplace sexual, verbal and physical harassment among headache professionals. Methods We adopted a cross‑sectional exploratory survey approach with quantitative design. The survey was distributed electronically among headache healthcare and research professionals globally through the International Headache Society (IHS). Results Data were obtained from 579 respondents (55.3%; 320/579 women). A large percentage of respondents (46.6%; 270/579) had experienced harassment; specifically, 16.1% (93/578) reported sexual harassment, 40.4% (234/579) verbal harassment and 5.5% (32/579) physical harassment. Women were almost seven times more likely to experience sexual harassment compared to men (odds ratio = 6.8; 95% confidence interval = 3.5–13.2). Although women did also more frequently report other types of harassment, this was not statistically significant (odds ratio = 1.4; 95% confidence interval = 1.0–2.0). Conclusions Lifetime exposure to workplace harassment is prevalent among headache professionals, especially in women. The present study uncovers a widespread issue and calls for strategies to be implemented for building a healthy and safe workplace environment.
A pioneer in the management of Australian psychiatric services: Dr Richard Greenup (1803–1866)
Authors: Brakoulias Vlasios , Buxton Megan , McDonald Tracy , White Richard T , De Moore Gregory 
Published: August 2023

Objectives To provide a brief biography of Dr Richard Greenup and to explore the legacy he has left to one of Australia’s longest operating psychiatric services. Methods This history was obtained by consulting staff working within Cumberland Hospital, New South Wales and by examining primary and secondary sources. Results Greenup was the second surgeon superintendent of ‘The Parramatta Lunatic Asylum’ from 1852 until 1866 when he was fatally stabbed with a pair of scissors by a patient. Greenup was involved in establishing The University of Sydney and advocated for expanded and improved services for people diagnosed with mental disorders. Conclusions Greenup recognised the needs of the mentally ill and sought to address similar social and demographic determinants of health to those that we face today. The challenges he faced, and his tragic end remind us of the importance of delivering patient-centred care whilst being mindful of associated risks.