bluuebunny/crossref_metadata_2025_split huggingface dataset:
Forecast of Turkey's Carbon Emissions Within the Framework of the European Union’s Green Deal
Authors: Terzioglu Mustafa , KAYAKUŞ Mehmet , ERDOGAN Dilsad 
Published: April 2023

Abstract
The most important of such efforts is the Paris Climate Agreement signed in 2015 and the t’s Green Deal, implemented by the European Union (EU) within the framework of this Agreement. The targets stated in Grean Deal include measures affecting not only the EU countries but also third countries with which the EU has foreign trade links. For this purpose, ın this study, the carbon emission of Turkey, which has serious commercial relations with the EU, was tried to be estimated using machine learning techniques and an estimate was made for the year 2030 on the basis of the results obtained. These results were evaluated in line with the targets of the Green Deal. The R2 value of Support Vector Regression (SVR), which is applied in the model as one of the machine learning techniques, was found to be 98.4% and it was found to have the highest predictive power. This technicque is followed by Multiple Linear Regression (MLR) with a 97.6% success rate and Artificial Neural Network (ANN) with 95.8% success rate, respectively. According to the estimates made with the most successful model, SVR, Turkey's carbon emissions are expected to be 723.97 million tons (mt) of CO2 in 2030, the target year set by the EU. This level is 42% more compared to the the target that needs be achieved given the data existing in 2019. In terms of the results obtained from the study, it is thought that the study could be an exemplary model for other countries that have commercial ties with the EU.
Cannabidiol inhibits inflammation in rat osteoarthritis
Authors: Yin Hai chang , Zhang Xin yu , Chen Zhuo , Yi Na na , Jiang Xinjie , Yu Tian fei 
Published: April 2023

Abstract
(1)Objective: To investigate the therapeutic effect of cannabidiol (CBD) on osteoarthritis (OA) in rats.
(2)Methods: We randomly divided 36 female SD rats with SPF values of 7 weeks into model control, CBD at elevated doses, CBD at low doses, solvent control, and PBS control. Sodium acetate OA was modeled in the model, experimental and solvent control groups. After modeling, the experimental group was injected daily with CBD and solvent, and the control group was injected with PBS. After 12 days of treatment, the OA grade was scored, and the rats were anesthetized, and the rats were sacrificed by neck dislocation, blood was collected from the heart, knee joints were collected, the degree of joint swelling was recorded, and serum inflammatory cytokines IFN-γ, IL-1β, TFN-α, IL-6 concentration were and pathological changes of knee joint.
(3）Results: Compared with the model group and the solvent control group, the pain and claudication of the rats in the CBD experimental group were effectively relieved, and the levels of inflammatory cytokines (IFN-γ, IL-1β, TFN-α) in serum and synovial fluid were significantly reduced. Arthritic pathologic changes in the knee were significantly reduced.
(4) Conclusion: CBD is effective in reducing blood inflammatory factors in OA rats, relieving pain and pathological changes, and has significant therapeutic effects in osteoarthritis.
Reasons for uncontrolled orbital tumors after Gamma Knife surgery
Authors: Wu Dong-fang , Deng Hao , He Wei-min 
Published: April 2023

Abstract
Background
To analyze the reasons for uncontrolled Gamma Knife surgery (GKS) of orbital tumors. If we can avoid selecting tumors that are easy to recur, the application of Gamma Knife in ophthalmology will be safer and more popular.
Methods
Retrospective clinical analysis of 72 patients with orbital tumors treated with GKS over a 10-year period from January 2010 to December 2020.The study included 29 males and 43 females with mean age of 42.3years (range 11–75 years).Collect the tumor type, tumor volume, clinical symptoms, GKS dose of all patients before GKS.25 cases were treated with surgery before GKS and had a clear pathological diagnosis; 47 cases were treated without surgery before GKS and a clinical diagnosis was made based on clinical features and imaging manifestations.
Results
After GKS, the volume increased in 39 cases, stabilized in 17 cases, and decreased in 16 cases.In the 16 cases with reduced volume, the average volume of was 3.35 (0.31–8.26) cm3; The median margin dose is 11Gy (7-14Gy), and the median isodose curve is 50% (45%-50%).Of the 39 cases with volume growth, the mean volume was 17.37 (3.19–50.91) cm3, the median margin dose was 12 Gy (7–16 Gy), and the median isodose curve was 50% (45%-65%).Of the 39 patients who grew in size, 20 were reoperated, 10 underwent GKS again, and the remaining 9 were under clinical observation.Of the 20 cases of surgery after GKS,7 cases in which the postoperative pathological diagnosis was inconsistent with the clinical diagnosis at the time of GKS.The average Ki67 index of the patients at the time of surgery after GKS was 7.7%.No serious acute side effects were observed after GKS.
Conclusions
The following factors affect the efficacy of GKS: 1. orbital tumor volume; 2. orbital tumor type and Ki67 index; 3. GKS dose and parameters.
Magnetic field filtering of the hinge supercurrent in unconventional metal NiTe2-based Josephson junctions
Authors: Qu Fanming , Le Tian , Zhang Ruihan , Li Changcun , Jiang Ruiyang , Sheng Haohao , Tu Linfeng , Cao Xuewei , Lyu Zhaozheng , Shen Jie , Liu Guangtong , Liu Fucai , Wang Zhijun , Lu Li 
Published: April 2023

Abstract
Topological materials with boundary (surface/edge/hinge) states have attracted tremendous research interest. Besides, unconventional (obstructed atomic) materials have recently drawn lots of attention owing to their obstructed boundary states. Experimentally, Josephson junctions (JJs) constructed on materials with boundary states produce the peculiar boundary supercurrent, which was utilized as a powerful diagnostic approach. Here, we report the observations of conspicuous hinge supercurrent in NiTe2-based JJs. Particularly, applying an in-plane magnetic field along the Josephson current could rapidly suppress the bulk supercurrent and retain the nearly pure hinge supercurrent, namely the magnetic field filtering of supercurrent. Further systematic comparative analysis and theoretical calculations demonstrate the existence of unconventional nature and obstructed hinge states in NiTe2. Our results revealed the unique hinge states in unconventional metal NiTe2, and demonstrated in-plane magnetic field as an efficient method to filter out the futile bulk contributions and thereby to highlight the hinge states hidden in topological/unconventional materials.
Exploring Differentially Expressed Genes of Staphylococcus Aureus Exposed to Human Tonsillar Cells Using Rna Sequencing
Authors: Bastakoti Srijana , Ajayi Clement , Julin Kjersti , Johannessen Mona , Hanssen Anne-Merethe 
Published: April 2023

Abstract
Background: The nose and the throat are the most predominant colonizing sites of Staphylococcus aureus, and colonization is a risk factor for infection. Nasal colonization is well described; however, we have limited knowledge about S. aureus throat colonization. The main objective of this study was to explore differentially expressed genes (DEGs) in S. aureus throat isolate TR145 exposed to human tonsil epithelial cells (HTEpiC) by using RNA sequencing (RNA-seq) and pathway analysis. DEGs in S. aureus at 1 h and 3 h interaction with its host were explored.
Results: All samples after 3 h of exposure showed more than 65 % of RNA reads uniquely mapped with its reference genome. Mapping efficacy at 1 h of exposure condition was identified to range from 20 % to 93 %. Downstream analysis of the obtained gene read counts, revealed putative transcripts expressed upon S. aureus exposure to tonsillar cells. A total of 508 DEGs were identified including unique (1 h, 160 DEGs and 3 h, 78 DEGs) and commonly shared genes (1h and 3h, 270 DEGs). Among the DEGs, were genes encoding proteins involved in adhesion and immune evasion, as well as iron acquisition and transport. Reverse transcription qPCR was done on selected genes, and the results correlated with the RNA-seq data.
Conclusion: We have shown the suitability of using HTEpiC as an in vitro model for investigating key determinants in S. aureus during co-incubation with host cells. Several DEGs were unique after 1 or 3 h exposure to host cells, while others were commonly expressed at both time points. As their expression is induced upon meeting with host, they might be explored further for future targets for intervention to prevent either colonization or infection in the throat.
Cost-effectiveness analysis of prophylactic laser peripheral iridotomy for primary angle-closure suspect in Japan
Authors: Fujita Asahi , Konishi Takaaki , Sakata Rei , Hashimoto Yohei , Yasunaga Hideo , Aiahra Makoto 
Published: April 2023

Abstract
Background/Objectives:
This study aimed to compare the cost-effectiveness of prophylactic laser peripheral iridotomy (LPI) with that of observation for primary angle-closure suspect (PACS) in Japan.
Subjects/Methods:
A Markov model was developed to compare the costs and utilities of prophylactic LPI with those of observation of 40-year-old patients with PACS. In the model with a yearly cycle over a 20-year time horizon, the disease was postulated to irreversibly progress from PACS to primary angle closure, followed by primary angle-closure glaucoma, unilateral blindness, and bilateral blindness. The parameters were estimated based on a recent randomised controlled trial and analyses of Japanese claims data. The incremental cost-effectiveness ratio was estimated from the healthcare payer’s perspective and evaluated as the willingness-to-pay 5 million Japanese Yen per quality-adjusted life-year. The observation period was shortened, and the age at entry into the cohort was changed to 50 years in the sensitivity analyses. We conducted one-way deterministic sensitivity analysis and probabilistic sensitivity analysis with Monte Carlo simulations with 10 000 iterations.
Results
The incremental cost-effectiveness ratio of LPI was 2 270 224 Japanese Yen (14 189 pounds sterling) per quality-adjusted life-year, which was below the willingness-to-pay threshold. The ratios were approximately 4 and 8 million in the 15-year and 10-year time horizons, respectively. Increasing the age at entry had little influence on the incremental cost-effectiveness ratio. The deterministic and probabilistic sensitivity analyses indicated that the results were robust.
Conclusions
Our results indicate that prophylactic LPI for middle-aged patients with PACS is cost-effective in Japan.
Protective effect of Allium jesdianum in an Alzheimer's disease induced rat model
Authors: Kamranfar Farzaneh , Jaktaji Razieh Pourahmad , Shirani Kobra , Jamshidi Amirhossein , Samiei Fatemeh , Arjmand Abdollah , Khoramjouy Mona , Faizi Mehrdad , Pourahmad Jalal 
Published: April 2023

Abstract
Aims: Alzheimer is a multifactorial disease that is caused by several different etiopathogenic mechanisms. The aim of this study is to evaluate the protective effects of Allium jesdianum extract on cognitive dysfunction, mitochondrial/cellular, and genetic parameters in Streptozotocin-induced Alzheimer's disease (AD) Rat Model.
Main methods: A single dose of STZ (3 mg/kg, i.c.v.) was injected to male Wistar rats in order to establish a model of sporadic AD. A. jesdianum extract (100,200, 400 mg/kg/day) and donepezil (5 mg/kg/day) were administered through oral gavage as treatment for 14 days after model induction. Cognitive function (radial arm water maze test), mitochondrial toxicity parameters consisting succinate dehydrogenase (SDH) activity, mitochondrial ROS formation, MMP decline, mitochondrial swelling and efflux of cytochrome c in various parts of the rat brain (whole brain, frontal cortex, hippocampus and cerebellum), and miR-330, miR-132, Bax and Bcl-2 genes expression in isolated rat brain neurons through RT-qPCR analysis were evaluated.
Key findings: A.jesdianum extract significantly attenuated i.c.v-STZ-induced cognitive dysfunction and mitochondrial upstream toxic events. As a result of STZ injection, Bax gene was highly expressed, whereas miR-330, miR-132 and Bcl-2 gene were poorly expressed and A. jesdianumreverses the expression of the above miRNAs and genes in favor of improving AD and reducing neuronal apoptosis.
Significance: A. jesdianum showed the neuroprotective capability against oxidative stress and cognitive impairment induced by STZ in rats shows its helpful therapeutic worth in AD.
Investigating the potential role of USP18 in atherosclerosis based on bioinformatics analysis
Authors: An Yang , Zhang Qiaojun , Liu Jiangjin , Zhou Hongmei , Xia Bing , Wan Chanwu , Lu Yanlin , Yu Yanni , Wang Jie , Dai Jialin 
Published: April 2023

Abstract
Background: Ubiquitin-specific protease 18 (USP18), is a member of the ubiquitin-specific protease family. Previous studies have shown that USP18 expression is upregulated in the patients with heart failure and USP18 is considered as a novel target for the treatment of heart failure. However, the role of USP18 in atherosclerosis remains unclear. In this study, we aimed to investigate the expression pattern of USP18 in atherosclerosis and its relationship between the extent of atherosclerotic and the cholesterol transporter protein ABCG1.
Methods: GSE6054 dataset was obtained from the Gene Expression Omnibus (GEO) database. Differentially expressed genes (DEGs) were analyzed by using the "limma" package in R (version 4.1.3). H-DOCK was applied to perform protein-protein docking for predicting the interaction between USP18 and ATP-binding cassette transporter protein G1 (ABCG1). Immunohistochemistry(IHC), immunofluorescence(IF), and Western blot were used to assess the protein expression of USP18 and ABCG1 in human coronary arteries. Dual immunofluorescencewas performed for co-localization analysis of USP18 and ABCG1
Results: Bioinformatics analysis identified 462 DEGs including 239 upregulated and 223 downregulated genes in familial hypercholesterolaemia (FH) patients, of which USP18 was upregulated in monocytes. Gene Ontology enrichment analysis indicated that the biological functions of USP18 were mainly enriched in endopeptidase activity and cytokine-mediated signaling. Protein-protein docking by H-DOCK showed that USP18 and ABCG1 interacted at a free energy of -20 kcal/mol (free energy < 0 was considered meaningful). USP18 expression is upregulated in patients with coronary artery disease and negatively correlates with the extent of atherosclerosis. At the same time, The expression of ABCG1 is downregulated in CHD and SCD patients and is more pronounced in SCD patients. In atherosclerotic tissues, USP18 and ABCG1 expression were positively correlated. In addition, double immunofluorescence assay showed co-localization of USP18 and ABCG1.
Conclusions: USP18 is a differentially expressed gene of FH, and its expression in atherosclerosis is closely related to the extent of atherosclerosis.
Integrative proteomics and metabolomics data analysis exploring the mechanism of brain injury after cardiac surgery in chronic stress rats
Authors: Yan Haoqi , Wang Hongbai , Chen Wenlin , Jia Yuan , Yan Fuxia , Yuan Su 
Published: April 2023

Abstract
Objective
Preoperative chronic stress (CS) is associated with postoperative brain injury in patients undergoing open heart cardiac surgery. This research is to explore the potential molecular biological mechanisms of brain damage following cardiac surgery in preoperative CS rats by the analyses combining proteomics and metabolomics.
Methods
We constructed the chronic unpredictable stress (CUS) and cardiac surgery models in adult rats. We proved the brain injury in CUS cardiac surgery rats by Hematoxylin-Eosin(H&E) staining, followed by separating the hippocampal tissue and investigating the potential mechanisms of brain injury by the methods of data-independent acquisition proteomics and untargeted metabolomics.
Results
The signaling pathways of glycoproteins and metabolism of amino acids were the main possible mechanisms of brain injury in CUS rats following cardiac surgery according to the proteomics and metabolomics alone. The association analysis of proteomics and metabolomics demonstrated the pathways of lysine degradation and β-alanine metabolism may be the main mechanism of cardiac surgery related brain injury in preoperative CUS rats.
Conclusions
The pathways of lysine degradation and β-alanine metabolism may be the potential mechanisms of brain injury in CUS rats following cardiac surgery. We should focus on the varieties of bioproteins and metabolites in these pathways, and related changes in other signaling pathways induced by the two pathways.
Effects of remimazolam combined with sufentanil on hemodynamics during anesthetic induction in advanced-age patients with hypertension undergoing orthopedic surgery of the lower limbs：a randomized,controlled trial
Authors: xu qiaomin , WU Jimin , SHAN Weifeng , DUAN Gongchen , LAN Haiyan 
Published: April 2023

Abstract
Purpose
This study was performed to observe the effect of remimazolam combined with sufentanil on hemodynamics during anesthetic induction in advanced-age patients with hypertension undergoing orthopedic surgery of the lower limbs.
Methods
This randomized controlled trial included 60 advanced-age patients with hypertension who were undergoing orthopedic surgery of the lower limbs. The patients were randomly assigned to undergo general anesthesia with either remimazolam (group R) or propofol (group P, n = 30, each group).The MAP,HR,CO,CI,SVR and pulse oxygen saturation were recorded. The disappearance time of the eyelash reflex, injection pain, hypotension, bradycardia, hiccuping, nausea and vomiting, and other adverse events were observed.
Results
The MAP, HR, CO, CI, and SVR were significantly higher in Group R than P at T1, T2, T3, and T4 (P < 0.05). In Group P, the MAP, HR, CO, and CI were significantly lower and the SVR was significantly higher at T1, T2, T3, and T4 than at T0 (P < 0.05). The disappearance time of the eyelash reflex was significantly longer in Group R than P, and the injection pain, hypotension, and bradycardia were significantly lower in Group R than P (P < 0.05). There was no significant difference in hiccupping or nausea and vomiting between the two groups (P > 0.05).
Conclusion
The combination of remimazolam and sufentanil for induction of general anesthesia produces only small hemodynamic fluctuation, maintains stability of the circulation, and has few adverse effects, making it more suitable for advanced-age patients with hypertension.
Haploinsufficiency of ZNF251 causes DNA-PKcs-dependent resistance to PARP inhibitors in BRCA1-mutated cancer cells
Authors: Li Huan , Chatla Srinivas , Liu Xiaolei , Vekariya Umeshkumar , Kim Dongwook , Walt Matthew , Lian Zhaorui , Morton George , Feng Zijie , Yang Dan , Liu Hongjun , Reed Katherine , Childers Wayne , Yu Xiang , Madzo Jozef , Chitrala Kumaraswamy Naidu , Skorski Tomasz , Huang Jian 
Published: April 2023

Abstract
Poly (ADP-ribose) polymerase (PARP) inhibitors represent a promising new class of agents that have demonstrated efficacy in treating various cancers, particularly those that carry BRCA1/2 mutations. The cancer associated BRCA1/2 mutations disrupt DNA double strand break (DSB) repair by homologous recombination (HR). PARP inhibitors (PARPis) have been applied to trigger synthetic lethality in BRCA1/2-mutated cancer cells by promoting the accumulation of toxic DSBs. Unfortunately, resistance to PARPis is common and can occur through multiple mechanisms, including the restoration of HR and/or the stabilization of replication forks. To gain a better understanding of the mechanisms underlying PARPi resistance, we conducted an unbiased CRISPR-pooled genome-wide library screen to identify new genes whose deficiency confers resistance to the PARPi olaparib. Our study revealed that ZNF251, a transcription factor, is a novel gene whose haploinsufficiency confers PARPi resistance in multiple breast and ovarian cancer lines harboring BRCA1 mutations. Mechanistically, we discovered that ZNF251 haploinsufficiency leads to constitutive stimulation of DNA-PKcs-dependent non-homologous end joining (NHEJ) repair of DSBs and DNA-PKcs-mediated fork protection in BRCA1-mutated cancer cells (BRCA1mut + ZNF251KD). Moreover, we demonstrated that DNA-PKcs inhibitors can restore PARPi sensitivity in BRCA1mut + ZNF251KD cells ex vivo and in vivo. Our findings provide important insights into the mechanisms underlying PARPi resistance and highlight the unexpected role of DNA-PKcs in this phenomenon.
Flipped Learning as an Educational Model in a Cardiology Residency Program
Authors: Sierra-Fernández Carlos Rafael , Huipe-Dimas Alejandra , Garnica-Geronimo Luis Rodrigo , Trevethan-Cravioto Sergio Arnulfo , López-Meneses Mauricio , Azar-Manzur Francisco Javier 
Published: April 2023

Abstract
Introduction: Flipped learning (FL) is a model which allows students to adjust their study rhythm by taking advantage of class time to apply their knowledge. Although FL meets many of the needs of the traditional lecture-based model and the exclusively virtual model, its effectiveness in medical residency programs has not been thoroughly studied. Our study evaluates the efficacy of an FL model in a cardiology residency program based on the satisfaction and academic performance of the participants. Method: A prospective, observational, and descriptive study was conducted at Ignacio Chávez National Institute of Cardiology in Mexico City to evaluate the satisfaction and effectiveness of an FL model for acquiring competencies in electrocardiographic diagnosis among thirty-one first-year cardiology residents. The Advanced Electrocardiography Workshop and a virtual classroom were designed for the intervention. Four teachers taught the workshop and video classes, and ten medical specialists from the participants’ areas of work performed evaluations of practical skills before and after the intervention.
Results: 75% of the participants rated the intervention as very favorable compared to the traditional and virtual models. The main advantages offered by the FL model were identified. An improvement of high statistical significance was observed in participants’ academic performance after the intervention (P value <0.001).
Conclusion:The FL model has a high degree of acceptance among participants and teachers. Our study shows an improvement in academic performance after the intervention. Considering that the FL model is flexible and reproducible in other areas of medicine, it represents an excellent educational alternative that meets current needs.