bluuebunny/crossref_metadata_2025_split huggingface dataset:
Genome Formula Changes During Whitefly Transmission of Two Bipartite Cassava Mosaic Begomoviruses
Authors: G Kennedy George , Sharpee William , Jacobson Alana L. , Wambugu Mary , Mware Benard , Hanley-Bowdoin Linda 
Published: March 2023

Abstract
Cassava mosaic disease is caused by a complex of whitefly-transmitted begomovirus species, which often occur in mixed infections. These viruses have bipartite genomes consisting of DNA-A and DNA-B that are encapsidated into separate virions. Individual virus species exist in plants and whitefly vectors as populations comprising both genome segments, which can occur at different frequencies. Both segments are required for infection, and both must be transmitted for virus spread to occur. Cassava plants infected with both cognate segments of African cassava mosaic virus (ACMV) and/or East African cassava mosaic Cameroon virus (EACMCV) were used to examine how titers of the segments in a plant relate to their respective probabilities of acquisition by whiteflies and to the titers of each segment acquired and subsequently transmitted by whiteflies. The relationship between the DNA-A:DNA-B ratio in the plant and the relative likelihood of acquiring each segment differed between ACMV and EACMCV. However, for both viruses, DNA-A:DNA-B ratios acquired by whiteflies differed from those in the source plant and the ratios transmitted by the whitefly were “1” – the ratio at which the highest probability of transmitting both segments is expected.
Experimental and Theoretical Evidence Pointing to a Possible Classical Value to Elementary Electric Charge
Authors: Williams Lamont 
Published: March 2023

Abstract

It is well known that electric and magnetic phenomena are closely related. It is also well established that the electron magnetic moment has a classical and quantum value, in which the quantum value is the classical value times a particular number, approximately 1.0012, that accounts for quantum mechanical effects. This study explores the possibility that elementary electric charge (e) also has a classical and quantum value. Data from experiments, as well as a quark-based theoretical study, that identified dual values for e were evaluated. Results show that across the studies, there was a surprisingly consist appearance of a factor of about 1.0015 (or some power of the number) by which the lower value of e in the studies needed to be multiplied to equal the higher value. This suggests that the lower value in each study was reflective of a classical e value, while the higher number began to account for quantum effects, with 1.0015 possibly being an anomalous factor associated with the two values. Interpretations of the findings are presented showing how the study results possibly lead to a fundamental equation for the fine-structure constant and provide plausible solutions to the Landau pole and quantum triviality problems of quantum electrodynamics, which are currently persistent complications in that theory.
Key person-centered care domains for residential substance use disorder treatment facilities: former clients’ perspectives
Authors: Andraka-Christou Barbara , Atkins Danielle , Shields Morgan C. , Golan Olivia K. , Totaram Rachel , Cortelyou Kendall , Lambie Glenn , Mazurenko Olena 
Published: March 2023

Abstract
Background
While person-centered care (PCC) includes multiple domains, residential SUD treatment clients may value certain domains over others. We sought to identify the PCC domains most valued by former residential SUD treatment clients.
Methods
We distributed an online survey via social media to a national convenience sample of former residential SUD treatment clients. Respondents were presented with ten PCC domains in an online survey: (a) access to evidence-based care; (b) integration of care; (c) diversity/respect for other cultures; (d) individualization of care; (e) emotional support; (f) family involvement in treatment; (g) transitional services; (h) aftercare; (i) physical comfort; and (j) information provision. Respondents were asked to select up to two domains they deemed most important to their residential SUD treatment experience. We used descriptive statistics to identify response frequencies and logistic regression to predict relationships between selected domains and respondents’ race, gender, relationship status, parenting status, and housing stability.
Results
Our final sample included 435 former residential SUD treatment clients. Diversity and respect for different cultures was the most frequently selected domain (29%), followed by integration of care (26%), emotional support (26%), and individualization of care (26%). Provision of information was the least frequently chosen domain (3%). Race and ethnicity were not predictive of selecting respect for diversity. Also, parental status, relationship status and gender were not predictive of selecting family integration. Employment and housing status were not predictive of selecting transitional services.
Conclusions
While residential SUD treatment facilities should seek to implement PCC across all domains, our results suggest facilities should prioritize (a) operationalizing diversity, (b) integration of care, and (c) emotional support. Significant heterogeneity exists regarding PCC domains deemed most important to clients. PCC domains valued by clients cannot be easily predicted based on client demographics.
Multicore-shell MnO2@Ppy@N-doped porous carbon nanofiber ternary composites as electrode materials for high- performance supercapacitors
Authors: Wang Yi , Wang Jie , Wei Dong , Xu Lan 
Published: March 2023

Abstract
In this study, a multicore-shell ternary composite electrode material (MnO2@Ppy@NPCNFs) with excellent electrochemical performances was prepared by using surface modification, in which core-shell Ppy@N-doped porous carbon nanofibers (Ppy@NPCNFs) with large specific surface area and high conductivity were used as the substrate (a multicore layer), and MnO2 was loaded on the substrate by hydrothermal synthesis to form a shell layer, further improving the SC of electrode material. The parameters of hydrothermal growth of MnO2 on Ppy@NPCNFs were explored by means of the control variable method and response surface methodology, and the optimal parameters were predicted and verified. Electrochemical test results showed that the SC of MnO2@Ppy@NPCNFs prepared under the optimal reaction parameters was as high as 595.77 F g-1, and its capacitance retention was 96.2% after 1000 cycles. Moreover, a symmetric supercapacitor prepared with the optimal multicore-shell electrode showed an energy density of 9.36 Wh kg−1 at a power density of 1000 W kg−1 and a retention rate of 93.75% after 1000 cycles, indicating the promising application of multicore-shell ternary composite electrode material in high-performance supercapacitors.
ESCO2 promotes hypopharyngeal carcinoma progression in a STAT1-dependent manner
Authors: Hu Juan , Yan Jing , Chen Yijie , Li Xiaohui , Yang Liu , Di Haiyu , Zhang Huihui , Shi Yewen , Zhao Junjie , Shi Yanxia , Xu Yinglong , Ren Xiaoyong , Wang Zhenghui 
Published: March 2023

Abstract
Background: The establishment of sister chromatid cohesion N-acetyltransferase 2 (ESCO2) is involved in the development of multiple malignancies. However, its role in hypopharyngeal carcinoma (HPC) progression remains uncharacterized.
Methods: This study employed bioinformatics to determine the ESCO2 expression in head and neck squamous cell carcinoma (HNSC) and normal tissues. In vitro cell proliferation, migration, apoptosis, and/or cell cycle distribution assays were used to determine the function of ESCO2 and its relationship with STAT1. Xenograft models were established in nude mice to determine ESCO2 in HPC growth in vivo. Coimmunoprecipitation/mass spectrometry (Co-IP/MS) was conducted to identify the potential ESCO2 binding partners.
Results: We found that ESCO2 expression was elevated in HNSC tissues, and ESCO2 depletion suppressed tumor cell migration in vitro and inhibited tumor growth in vitro and in vivo. Co-IP/MS and immunoblotting assays revealed the interaction between ESCO2 and STAT1 in HPC cells. ESCO2-mediated suppressive effects on HPC cell proliferation, viability, and migration were compromised by STAT1-overexpression.
Conclusions: These findings suggest that ESCO2 is crucial in promoting HPC malignant progression through the STAT1 pathway, and provide novel therapeutic targets for HPC treatment.
Pharmacological inhibition of STING signaling attenuates MPTP-induced neuroinflammation and neurodegeneration in mouse parkinsonian models
Authors: Wang Baozhu , Qiu Jingru , Wang Yanwei , Gao Shixuan , Yu Shuyan , Sun Deqing , Lou Haiyan 
Published: March 2023

Abstract
Background
Parkinson's disease (PD) is a progressive neurodegenerative movement disorder characterized by the loss of nigrostriatal dopaminergic neurons and chronic neuroinflammation. However, the mechanisms linking chronic neuroinflammation to dopaminergic neuronal death have not been completely elucidated. Recent emerging evidence reveals that the cGAS-STING-mediated Type I interferon (IFN) signaling axis takes part in the microglial-associated neuroinflammation. However, the potential role of pharmacological inhibition of STING on neuroinflammation and thus the dopaminergic neurodegeneration is largely unknown.
Methods
In vitro, the effects of pharmacological inhibition of STING on LPS/MPP+-induced inflammatory responses in BV2 microglial cells were determined by real time RT-PCR and western blot analysis. For the in vivo studies, the acute and sub-acute mice models of PD were established by intraperitoneal injection of 1-methyl-4-(2′-methylphenyl)-1,2,3,6-tetrahydrophine (MPTP). The selective STING inhibitor C-176 was administered by intraperitoneal injection. The potential protective effects of C-176 on dopaminergic neurons and neuroinflammation were evaluated by behavioral test, tyrosine hydroxylase (TH) immunostaining, Nissl staining, western blotting, qPCR and immunofluorescence.
Results
We report that STING levels were upregulated in MPTP-induced PD mice model. Therapeutic inhibition of STING with C-176 significantly inhibited the activation of downstream signaling pathway, suppressed associated neuroinflammation, and ameliorated MPTP-induced dopaminergic neurotoxicity and motor deficit. Furthermore, pharmacological inhibition of STING within BV2 microglia which treated with LPS/MPP+ leads to decreased inflammatory responses. More importantly, C176 also reduced NLRP3 inflammasome activation both in vitro and in vivo.
Conclusion
The results of our study suggest that pharmacologic inhibition of STING protects against neuroinflammation that may act at least in part through suppressing NLRP3 inflammasome activation and thus ameliorated dopaminergic neurodegeneration. STING signaling may holds great promise for the development of new treatment strategy for PD as an effective therapeutic target.
Comparison of cervical sagittal parameters among patients with neck pain and patients with cervical spondylotic radiculopathy and cervical spondylotic myelopathy
Authors: Liu Tao , Tian Shuo , Zhang Jianzhou , He Mengzi , Deng Longlian , Ding Wenyuan , Wang Zheng , Yang Dalong 
Published: March 2023

Abstract
Purpose: Previous studies have shown that cervical sagittal alignment is strongly associated with cervical deformity, myelopathy,and cervical adjacent-segmental disease, and those cervical sagittal parameters are correlated with health-related quality of life, while less attention has been paid to cervical sagittal balance in various cervical disorders. This study aimed to compare cervical sagittal parameters between patients with nonspecific neck pain(NS-NP) and patients with cervical spondylotic radiculopathy(CSR) and cervical spondylotic myelopathy(CSM).
Methods: We retrospectively included 236 patients divided into three groups, NS-NP, CSR, and CSM, and collected general information and cervical sagittal parameters of such kind patients. The variation of parameters between the size of these parameters and gender differences were analyzed. Pearson’s or Spearman’s correlation was applied to analyzethe association of cervical sagittal parameters of all patients between the three groups.
Results: There were significant differences in age and sex among the three groups (P<0.001), among which the NS-NP group was the youngest, and NS-NP was more common in women. The parameters of cervical sagittal position were significantly different among the three groups (P<0.05). Pearson’s or Spearman’s correlation result showed that the C2-C7 Cobb angle was negatively associated with C2-C7 SVA, and the C2-C7 Cobb angle, T1 slope (T1s) were negatively associated with the spino-cranial angle (SCA). There was a positive correlation between the C2-C7 Cobb angle and C7 slope (C7s), C2-C7 SVA and T1s, C2-C7 SVA and SCA, and C7s and T1s.
Conclusion: This study showed that between the three groups, patients with non-specific neck pain had larger C7s, T1s, and C2-C7 Cobb angle and smaller SCA and C2-C7 SVA; and among patients with NS-NP, women had larger SCA and smaller C7s and T1s. The smaller anteroposterior (AP) diameter of the thoraxin women might be the possible explanation for this difference.
Synthesis, in-silico based virtual screening,anti-cancer potential of novel1,2,3-triazole-thiadiazole hybrid derivatives as Aurora kinase A (ARK-A) and Extracellular regulated kinase 2 (ERK2) dual inhibitors.
Authors: Bontha Lingaiah , E Praveen Kumar , Dokala Appaji , Pingili Divya , Putta Venkat Reddy , Vuradi Ravi kumar , Kotha Laxma Reddy , Satyanarayana sirasani 
Published: March 2023

Abstract
As part of our ongoing efforts to produce promising cytotoxic agents, the novel compounds, 5-(4-(diethylamino)-2-((1-substitutedphenyl-1H-1,2,3-triazol-4-yl)methoxy)phenyl)-1,3,4-thiadiazol-2-yl)pyrrolidine-2,5-dione derivatives (9a-l) were developed, synthesised, and characterized using several analytical techniques, including 1H NMR, 13C NMR, and LC-MS. New series of 1,2,3-triazole and thiadiazole molecular hybrids synthsized were evaluated for their anticancer activity against human oesophageal carcinoma cell line KYSE-450 and human pancreatic carcinoma cell line MIA PaCa-2 cells. The compounds 9b, 9i, 9j, and 9l exhibited potential cytotoxic activity against KYSE-450 and MIAPaCa-2 cells, according to cytotoxic evaluation data. Compound 9j had greater anti-cancer potential relative to the standard employed across all compounds evaluated. The remaining compounds exhibited moderate to weak anti-proliferative potential. In-vitro kinase inhibition of compound 9j was significantly more effective against both ARK-1 and ERK-2 enzymes, indicating its dual inhibition potential. Docking analysis culminated that 9k, 9j, and 9i have substantial docking scores with the ARK-1 receptor, indicating the presence of strong binding affinities. Significant binding interactions between molecules 9j and 9h and the ERK-2 receptor suggest an inhibitory effect. Hence the compounds are promising dual inhibitors of ARK-A/ERK2.
Developing River Tourism on the Petagas River, Sabah: Understanding Community Perceptions and Potential Level of Engagement
Authors: Saikim Fiffy Hanisdah , Phang Grace Ing , Pawan Marry Tracy , Kadar Nazia Abdul , Hussein Muhammad Ali Syed , Salleh Norhuda , Nordin Nordiana Mohd. , Aziz Nor Akmar Abdul , Prideaux Bruce 
Published: March 2023

Abstract
Pollution-free rivers with protected riverbanks offer considerable scope for tourism development. In recent decades there has been increasing interest in rivers as a tourism resource. Globally, the demand for river cruising and water sports has grown rapidly as has the development of riverbanks for tourism purposes including accommodation, restaurants, and shopping. However, the role of local communities attitudes and perceptions in the development of river tourism is crucial to developing a successful tourism resource. The purpose of this study was to investigate the perceptions of local residents regarding the development of river tourism on the Petagas River, Kota Kinabalu, Sabah, Malaysia. Face-to-face semi-structured interviews were undertaken with 79 residents of river communities to explore their perceptions of river tourism development on the Petagas River, assess their views on their likely participation in river tourism, and identify problems they thought might inhibit tourism development. The results show that while respondents were generally supportive of tourism development, they did recognise the need for river ecosystem restoration and the need to change many of the ways they currently use the river.
Hugely improved electromagnetic interference shielding and mechanical properties for UHMWPE composites via constructing an oriented conductive carbon nanostructures (CNS) networks
Authors: Cheng Huibin , Lin Guoliang , Zhang Xiaoyi , Wu Chen , Ma Shenglan , Liu Xuhong , Huang Baoquan , Chen Qinghua , Qian Qingrong , Cao Changlin 
Published: March 2023

Abstract
To address the practical application challenges of conductive polymer composites (CPCs) in portable electronics equipment, such as their low thermal conductivity (TC) and poor electromagnetic interference (EMI) shielding effectiveness (EMI SE), it is crucial to improve their TC, electrical conductivity(σ), and EMI SE of CPCs. In this work, we present a conducting composite made of ultrahigh molecular weight polyethylene (UHMWPE) and carbon nanostructures (CNS) with a unique segregated structure. This structure is achieved through a simple high-speed mechanical mixing and compression molding process. Microscopy characteristics demonstrated that both the matrix and segregated conductive network were in-situ oriented along the compress direction of UHMWPE granules under the static hot-pressing field. CNS are compacted together at the interface between UHMWPE granules to form an oriented and interconnected conductive pathways at low CNS loading levels. The resultant UHMWPE/CNS composites with 10 wt% CNS content exhibits excellent EMI shielding performance, with EMI SE of 60.7 dB (at X-band), high conductivity of 2.42 S/cm, and acceptable thermal conductivity of 0.7217 (W/m K). High EMI shielding performance and absorption dominant mechanism are beneficial from the unique segregated structure, and individual CNS coated UHMWPE granule are similar to an electromagnetic cage. Additionally, the ultimate tensile strength of the composite remains high at 37.6 MPa even at 10.0 wt% CNS loading, and it shows effective thermal stability. These properties are attributed to the strong interfacial bonding between CNS and UHMWPE. These materials have potential applications in efficient thermal management and EMI shielding for high-performance intelligent electrical devices.
Triassic palynology of the Swiss Belchentunnel - a restudy of the Scheuring samples
Authors: Schneebeli Elke , Kustatscher Evelyn 
Published: March 2023

Abstract
Well-preserved Carnian (Late Triassic) palynomorphs are rare in Switzerland, despite sediments include one of the important plant fossil localities, Neue Welt near Basel. Modern detailed palynological studies on Triassic palynomorphs in general and especially in the Carnian are scarce, most palynological studies have been carried out more than fifty years ago. Nevertheless (Late) Triassic sediments still yield surprises for palynological research. Here, we present the results of the re-study of the famous Belchentunnel samples that have been studied and published by Bernhard Scheuring in 1970. The less cheerful result concerns the preservation of slides: more than 60% of the slides are degraded. On the other hand, the restudy of the well-preserved slides showed an unexpected number of algae, acritarch and spore taxa, not described so far. The latter facilitate the correlation with the well-known biostratigraphic schemes established for the Germanic Basin. The distribution of Porcellispora longdonensis throughout the Belchentunnel succession is especially striking. The acme just below the Schilfsandstein might suggest the presence of ephemeral ponds.
HPV-positive murine oral squamous cell carcinoma: development and characterization of a new mouse tumor model for immunological studies
Authors: Modic Ziva , Cemazar Maja , Markelc Bostjan , Cör Andrej , Sersa Gregor , Brezar Simona Kranjc , Jesenko Tanja 
Published: March 2023

Abstract
Background
Infection with high-risk human papillomavirus (HPV) strains is one of the risk factors for the development of oral squamous cell carcinoma (OSCC). Some patients with HPV-positive OSCC have a better prognosis and respond better to various treatment modalities, including radiotherapy or immunotherapy. However, due to the species specificity of HPV, there are only a few immunocompetent mouse models available that enable immunological studies. Therefore, the aim of our study was to develop a transplantable immunocompetent mouse model of HPV-positive OSCC and characterize it in vitro and in vivo.
Methods
Two monoclonal HPV-positive OSCC mouse cell lines were established by inducing the expression of HPV-16 oncogenes E6 and E7 in the MOC1 OSCC cell line using retroviral transduction. After confirming stable expression of HPV-16 E6 and E7 with quantitative real-time PCR and immunofluorescence staining, the cell lines were further characterized in vitro using proliferation assay, wound healing assay, clonogenic assay and RNA sequencing. In addition, tumor models were characterized in vivo in C57Bl/6NCrl mice in terms of their histological properties, tumor growth kinetics, and radiosensitivity. Furthermore, immunofluorescence staining of blood vessels, hypoxic areas, proliferating cells and immune cells was performed to characterize the tumor microenvironment of all three tumor models.
Results
Characterization of the resulting MOC1-HPV cell lines and tumor models confirmed stable expression of HPV-16 oncogenes and differences in cell morphology, in vitro migration capacity, and tumor microenvironment characteristics. Although the cell lines did not differ in their intrinsic radiosensitivity, one of the HPV-positive tumor models, MOC1-HPV K1, showed a significantly longer growth delay after irradiation with a single dose of 15 Gy compared to parental MOC1 tumors. Consistent with this, MOC1-HPV K1 tumors had a lower percentage of hypoxic tumor area and a higher percentage of proliferating cells. Characteristics of the newly developed HPV-positive OSCC tumor models correlate with the transcriptomic profile of MOC1-HPV cell lines.
Conclusions
In conclusion, we developed and characterized a novel immunocompetent mouse model of HPV-positive OSCC that exhibits increased radiosensitivity and enables studies of immune-based treatment approaches in HPV-positive OSCC.
CDS With Adjustable Interval for Low Noise Hyperspectral Infrared Focal Plane Readout circuits
Authors: Wu Shuang , Ding Ruijun , Chen Honglei , Liang Qinghua 
Published: March 2023

Abstract
Low noise is a key requirement of Readout Integrated Circuit (ROIC) in hyperspectral application for its low radiation. The Correlated Double Sampling (CDS) technology is commonly used to suppress the effect of noise. In this paper, CDS is improved by adjusting the time interval between the clamp and sample hold (SH), which can filter low frequency noise flexibly. A ROIC input stage integrated with low-noise Capacitive trans-impedance amplifiers (CTIA) and CDS with adjustable interval (AICDS) is designed. The SPECTRE simulation indicates that, by extending the CDS reset time, the simulation result is consistent with the analysis. The number of noise electrons reaches 32.59 e-, which is better than 56.7 e- without extending the reset time. The design of AICDS can improve the noise performance of hyperspectral ROIC, and can be widely used.