bluuebunny/crossref_metadata_2025_split huggingface dataset:
Antimalarial activity of Mangifera indica aqueous extract in Plasmodium berghei’s apicoplast
Authors: Asanga Edet E. , Okoroiwu Henshaw , Edet Uwem O. , Amaechi Dennis , Nelson Promise E. , Uchenwa Mercy , Eseyin Olorunfemi A. , Samuel Goodnews , Ettah Lordswill A. , Obongha Osuake A. 
Published: June 2023

Purpose: To investigate the in vivo antiplasmodial effect of Mangifera indica aqueous extract in Plasmodium berghei-infected mice, and to characterize its phytochemical constituents and their mechanism of action.
Methods: The plant leaves (250.71 g) and stem bark (509.34 g) were weighed, macerated in 5 L of boiled, distilled water for 72 h, and filtered. In vivo antiplasmodial evaluation of the extract was carried out using Plasmodium berghei-infected mice. The leaf and stem bark extracts were subjected to gas chromatography-mass spectroscopy (GC-MS) analysis for characterization of their chemical constituents.
Results: The leaf/stem bark (1:2) extract (88.27 %) exhibited chemosuppressive activities that was better than artesunate (88.04 %) and artemisinin-based combination therapy (ACT, 84.60 %). Its schizonticidal activities (98.63 %) revealed a better therapeutic response when compared with artesunate (92.34 %). Spectroscopic analysis showed linoleic acid esters and other compounds.
Conclusion: Therapeutic response of leaf/stem bark (1:2) extract exhibit superior schizonticidal activity to ACT and artesunate. Furthermore, the linoleic acid esters in Mangifera indica aqueous extract may contribute to strong antimalarial activity by inhibiting fatty acid biosynthesis.
Chaihu-Longgu-Muli Decoction promotes sleep in mice with insomnia
Authors: Wang Pei , Xin Siyuan , Wang Zheng , Yu Man , Wang Jiao , Li Jinfeng , Li Min 
Published: June 2023

Purpose: To determine the hypnotic and sedative effects, and the mechanism of action of ChaihuLonggu-Muli Decoction (CLMD) in mice with insomnia.
Methods: A mice model induced with insomnia using p-chlorophenylalanine (PCPA), was established. The sedative and hypnotic effects of CLMD on mice were evaluated by animal behavioral tests. The animals were separated into four experimental groups: (1) control group, (2) PCPA group, (3) PCPA + 5 mg/kg CLMD group, and (4) CPA + 10 mg/kg CLMD group. The 5-HTR1A expression was determined by western blot. The expression levels of the neurotransmitters were assessed using commercial ELISA kits, while the histological changes in hypothalamic tissues were examined microscopically after staining with hematoxylin and eosin (H & E).
Results: CLMD exerted a sedative effect on and ameliorated anxiety-like and stressful behaviors in mice with insomnia. Moreover, CLMD induced sleep and enhanced 5-HTR1A to modulate neurotransmitter secretion. The data also indicate that CLMD exerted a protective role by alleviating hypothalamic damage and regulating the activation of NLRP3 inflammasome associated with insomnia.
Conclusion: These findings indicate that CLMD has sedative and hypnotic effects, and offers a new therapeutic modality for insomnia treatment.
Effectiveness of nab-paclitaxel versus traditional paclitaxels in the treatment of ovarian cancer
Authors: Ding Jing , Xu Wei , Cheng Xiao-yan , Chen Xi-hai , Zhou Wen-jun , Ma Rong , Meng Fan-ling 
Published: June 2023

Purpose: To evaluate the effect of four taxane drugs, namely, paclitaxel, docetaxel, paclitaxel liposomes (Lipusu), and nab-paclitaxel (Keaili) on ovarian cancer cells both in vivo and in vitro.
Methods: BALB/c-nu/nu female mice were used to develop mouse xenograft models. The mice were randomized to 5 groups (4 in each group), namely, control (PBS) group, paclitaxel group, docetaxel group, liposomal paclitaxel group and nab-paclitaxel group. The effect of four taxane drugs were determined via cell proliferation and toxicity tests. Mouse xenograft models were employed to assess the efficacy of four taxane drugs in inhibiting tumor growth.
Results: Nab-paclitaxel has a significant ovarian growth-reducing effect in vitro. In vivo, no significant differences were observed in tumor volume among the four groups (p < 0.05). Nab-paclitaxel produced the lowest animal toxicity when compared with other three drugs as the mice in nab- paclitaxel treatment group showed no significant alterations in body weight (p < 0.05). Hematoxylin and eosin (H & E) staining revealed the lowest degree of liver tissue damage in mice treated with nab-paclitaxel compared to mice administered the other three paclitaxels.
Conclusion: Nab-paclitaxel is more effective in mice with ovarian cancer than traditional paclitaxels. Thus, it promises to offer a viable alternative as first- line chemotherapy for epithelial ovarian cancer in humans, as it has low systemic toxicity and fewer hypersensitivity reactions. However, further investigations, including clinical trials in humans, are required.
Efficacy of combined PD-1 immune therapy and chemotherapy in the treatment of locally advanced nonsmall cell lung cancer
Authors: Li Yongjie , Fu Dunzhi , Li Biao , Zhou Yuan , Li Fan , Wang Qiongyu , Huang Jialei 
Published: June 2023

Purpose: To investigate the clinical efficacy of anti-programmed cell death-1 (PD-1) immunotherapy in combination with chemotherapy in patients suffering from locally advanced non-small cell lung cancer (NSCLC).
Methods: A total of 110 NSCLC patients admitted in The Second Affiliated Hospital of Hainan Medical University, Haikou, China were randomly divided into two groups using envelope method. The control group was administered pemetrexed and cisplatin, and the study group was further treated with nivolumab. Each treatment cycle lasted for 21 days, and a total of 6 cycles were administered for the duration of therapy. The short-term objective response rate, adverse reactions, programmed cell death5 (PDCD-5), tumor necrosis factor-α (TNF-α), tumor necrosis factor-β1 (TGF-β1), and immune status indices were determined.
Results: The objective remission rate in study group was 79.25 % (42/53), which was higher than that in control group (61.40 %, 35/57; p < 0.05). After treatment, both groups showed higher levels of PDCD5 and TNF-α compared to their pre-treatment values, while demonstrating lower levels of TGF-β1. Furthermore, PDCD-5 and TNF-α in study group were higher than those in control group, while TGF-β1 was lower than that in control group (p < 0.05). In control group, the values of clusters of differentiation 3+(CD3+), 4+(CD4+), and 8+(CD8+) decreased after treatment, while the values of CD8+ increased after treatment. The CD3+, CD4+, and CD4+/CD8+ in study group were higher than those in control group, while CD8+ was lower than those in control group (p < 0.05).
Conclusion: Anti-PD-1 immunotherapy combination with chemotherapy in the treatment of local NSCLC stabilizes the immune system and improves the short-term efficacy of patients with advanced NSCLC. Further clinical trials are, however, required prior to application of this combination clinical practice.
Synaptopodin 2 represses cervical cancer cell growth and enhances the sensitivity of cervical cancer cells to cisplatin via Hippo pathway
Authors: Chen Yuanhang , He Lang , Xu Yi , Feng Qianqian 
Published: June 2023

Purpose: To explore the functions and regulatory mechanisms of synaptopodin-2 (SYNPO2) in cervical cancer progression.
Methods: Normal cervical cell lines (Ect1/E6E7) and cervical cancer cell lines (HeLa, SiHa, C-33A, and CaSki) were cultured. The pcDNA3.1 vector overexpressing SYNPO2, a negative control (vector), and blank control (control) were transfected into HeLa and SiHa cells. Protein expression from normal and cervical cancer cell lines was examined by western blot. Cell viability and proliferation were evaluated in HeLa and SiHa cells using Cell Counting Kit-8 and colony formation assays, while cell migration and invasion were assessed by wound healing and Transwell assays, respectively. Cell apoptosis was determined by flow cytometry.
Results: SYNPO2 expression was decreased in cervical cancer based on the Gene Expression Profiling Interactive Analysis website (p < 0.05). Additionally, Kaplan–Meier Plotter website showed that cervical cancer patients with low SYNPO2 expression showed worse prognoses than patients with high SYNPO2 expression (p < 0.05). Subsequent investigations revealed that SYNPO2 overexpression repressed cell proliferation, migration, and invasion in cervical cancer (p < 0.01). Furthermore, SYNPO2 overexpression enhanced cervical cancer cell apoptosis (p < 0.001) and increased the sensitivity of cervical cancer cells to cisplatin (p < 0.01). The regulatory function of SYNPO2 on Hippo pathway in cervical cancer indicate that SYNPO2 inactivated Hippo pathway (p < 0.05).
Conclusion: Synaptopodin 2 represses cervical cancer cell growth and enhances the sensitivity of cervical cancer cells to cisplatin via Hippo pathway, thus indicating its potentials for development for the treatment of cervical cancer.
Effectiveness of combined use of sodium bicarbonate sitz bath prescription and vaginal nystatin suppository in the treatment of patients with fungal vaginitis
Authors: Tao Zehong , Ye Aihua 
Published: June 2023

Purpose: To investigate the clinical effect of a combination of sodium bicarbonate sitz bath prescription and vaginal nystatin suppository in the treatment of patients with fungal vaginitis.
Methods: Ninety patients with mycotic vaginitis admitted to Tianchang Hospital of Traditional Chinese Medicine from April 2018 to April 2019 were selected and divided into control group (vaginal sitz bath with 5 % sodium bicarbonate solution, n = 45) and study group (vaginal nystatin suppository in addition to vaginal sitz bath with 5 % sodium bicarbonate solution, n = 45) based on the order of admission. The therapeutic effectiveness of the two treatments was compared with respect to time of relief of various clinical indicators, incidence of adverse reactions, rate of recurrence after 3 and 6 months of drug withdrawal, and vaginal pH.
Results: The time taken to relieve itching was longer in the control group (5.21 ± 2.12 days) than in the study group (3.74 ± 2.03 days; p <0 .05). There was a higher recurrence rate of itching in the control group after drug withdrawal for 3 and 6 months than in the study group (p < 0.05). Total treatment effectiveness was significantly higher in the study group than in the control group (p <0 .05). However, the incidence of adverse reactions was comparable in the two groups (p > 0.05). Vaginal pH significantly improved (less acidic) in the study group, when compared to the control group (p < 0.05).
Conclusion: Application of a combination of vaginal sitz bath using 5 % sodium bicarbonate solution and vaginal nystatin suppository is effective in the treatment of fungal vaginitis. The combination produces a favorable vaginal pH environment and low recurrence of infection. However, the combined treatment should be subjected to further clinical trials prior to application in clinical practice.
Satisfaction and quality of life of patients treated with tobramycin-dexamethasone suspension in daytime surgery managed by integrated nursing model
Authors: Yu Bo , Qiao Lingfei , Meng Yang , Li Ying , Shi Chunsheng 
Published: June 2023

Purpose: To investigate the effect of an integrated nursing model in day surgeries, treated with tobramycin-dexamethasone suspension, and its influence on patient satisfaction, quality of life, and indicators of immunity recovery.
Methods: Ninety-eight patients with ophthalmic diseases admitted to Anhui No. 2 Provincial People’s Hospital, Anhui, China from January 2021 to June 2022 were scheduled for surgery and treated with tobramycin-dexamethasone. They were randomly divided into two groups (control and study groups, with 49 cases in each group). All patients received routine nursing intervention, and an integrated nursing model was applied to the study group on this basis. Improvement in ocular symptoms, SF-36, changes in immune-related lymphocyte subsets in peripheral blood, and nursing satisfaction were compared.
Results: Ocular symptom score at 24 h after operation in the study group was lower than in control group (p < 0.05). The SF-36 scores (physical status, cognitive ability, social function, emotional and mental status), immune-related lymphocyte subsets, and overall satisfaction rate after intervention in the study group were higher than those in the control group (p < 0.05).
Conclusion: Integrated nursing model achieves ideal nursing effect in day surgery managed with tobramycin-dexamethasone> It also facilitates the recovery of patient’s eyes and plays a significant role in improving the quality of life, restoration of immunity, and nursing satisfaction of patients. A multicenter investigation will, however, be required prior to its full-scale clinical application.
The role of metformin in the management of endometriosis: A systematic review and meta-analysis
Authors: Zhang Heling , Liu Fang , Huang Yanzhen , Liu Wei 
Published: June 2023

Purpose: To analyze the activity of metformin in endometriosis, with a view to alleviating disease progression.
Methods: A search was conducted using electronic databases such as CNKI, VIP, CBM, WANFANG, Embase, PubMed, Cochrane, and Web of Science from January 1980 to January 2022 for randomized trials involving any intrapartum fetal surveillance method. A network meta-analysis was performed within a frequentist framework, and it was assessed for quality and network inconsistency of the trials. A ninety-five percent confidence interval (CI) was used to report the relationship between metformin levels and patients with endometriosis.
Results: Eight eligible studies which included 688 patients were evaluated in this meta-analysis. The primary indicators were treatment efficiency and CA-125 level. Metformin significantly increased treatment efficiency (SMD = 3.29, 95 % CI 2.01, 5.40, p < 0.00001). As expected, metformin also reduced the CA-125 level in endometriosis patients as a result (Odds Ratio, OR = -1.20, 95 % CI: -1.40 - 1.00, p < 0.00001).
Conclusion: Metformin alleviates pains caused by endometriosis. However, some studies had scanty information about alleviating clinical symptoms other than basic medical research. More randomized controlled trials are needed to confirm treatment efficacy.
CircRTN4 inhibits the progression of gastric cancer by sponging miR-424-5p and regulating LATS2 expression
Authors: Li Xiaoli , Wang Tianming , Chen Qian , Fu Qi , Cui Yan , Pan Haibang 
Published: June 2023

Purpose: To determine the expression of circRTN4 in gastric cancer (GC) and its role in tumor progression.
Methods: GEO dataset GSE93541 was analyzed using GEO2R. Starbase website was used to predict the combination of miRNA and circRTN4. The relationship between circRTN4 and prognosis was analyzed using Kaplan-Meier Plotter database, while expression levels of circRTN4, miR-424-5p, and LATS2 were assessed by quantitative real time-polymerase chain reaction (qRT-PCR). CCK8, EDU, Transwell, and Western blot were used to assess GC proliferation, migration, invasion, and stemness. Lastly, co-transfection of miR-424-5p or si-LAST2 was reversely used to demonstrate the regulatory effect of circRTN4 on the progression of gastric cancer cells. Significantly downregulated circRTN4 in GC was screened, and the combined miR-424-5p and downstream gene LATS2 were predicted by Starbase.
Results: The average relative expression of circRTN4 mRNA in GC tissues was significantly lower than in adjacent tissues. MiR-424-5p was highly expressed in tumor tissues, while LATS2 decreased (p < 0.05). Low expression of circRTN4 was associated with a low survival rate in patients. pLCDH- circRTN4 significantly inhibited the proliferation of gastric cancer cells (p < 0.05). Overexpression of circRTN4 inhibited the migration and invasion of tumor cells, while pLCDH-circRTN4 reduced the ability of GC stem cells and expressions of MMP2 and OCT4.
Conclusion: Expression of circRTN4 decreases in GC, and contributes to the development and progression of this disease by increasing the levels of LATS2 and binding with miR-424-5p. This suggests that circRTN4 may serve as a promising prognostic marker as well as a potential therapeutic target for gastric cancer
GAMBARAN NILAI TROMBOSIT PADA PASIEN TUBERCULOSIS PARU YANG MENDAPAT PAKET OBAT ANTI TUBERKULOSIS (OAT) DI RUMAH SAKIT UMUM DAERAH KOTA KENDARI
Authors: Yusuf Muhammad Ilyas , Firdayanti Firdayanti , Salmawati Salmawati 
Published: June 2023

ABSTRACT
Tuberculosis (TB) is an infectious disease that attacks the lungs caused by the bacteria Mycobacterium tuberculosis is transmitted through the air. One of the drugs used for TB disease rifampisin known as a cause of thrombocytopenia. This study aims to describe the number of platelets in patients receiving antituberculosis treatment medication package (OAT) in the General Hospital of City of Kendari. This type of research is descriptive , Automatic method. Based on the results of 18 samples of normal platelet values ​​obtained on the duration of treatment 2-6 months as many as 15 people (83.3%), while acquired decreased platelet count (thrombocytopenia) in the old first month of treatment as many as three people (16.7%).
Keywords :Tuberculosis lung, Anti tuberculosis drug, Platelet, RSUD Kendari city

ABSTRAK

Tuberkulosis (TB) adalah penyakit infeksi yang menyerang paru-paru yang disebabkan oleh kuman Mycobacterium tuberculosis yang penularannya melalui udara. Salah satu obat yang digunakan untuk penyakit TB yaitu rifampisin yang diketahui sebagai penyebab trombositopenia.Penelitian ini bertujuan untuk mengetahui gambaran nilai trombosit pada pasien tuberculosis paru yang mendapat perawatan paket obat anti tuberkulosis (OAT) di Rumah sakit Umum Daerah Kota Kendari. Jenis penelitian ini adalah deskriptif, pemeriksaan jumlah trombosit dilakukan menggunakan metode automatik.Berdasarkan hasil penelitian dari 18 sampel diperoleh nilai trombosit normal pada pasien dengan lama pengobatan 2-6 bulan sebanyak 15 orang (83,3%), sedangkan nilai trombosit tidak normal (trombositopenia) pada pasien dengan pengobatan 1 bulan sebanyak 3 orang (16,7%) .
Kata kunci : Tuberkulosis paru, obat anti tuberkulosis, trombosit, RSUD Kota Kendari
UJI DAYA HAMBAT EKSTRAK DAUN KEDONDONG (Spondias dulcis L.) TERHADAP PERTUMBUHAN Escherichia coli ATCC 35218
Authors: Fusvita Angriani , Aulya Muhammad Sultan 
Published: June 2023

ABSTRAK Kedondong merupakan tumbuhan tropis yang merupakan famili dari Anacardiaceae. Tanaman Kedondong digunakan sebagai obat tradisional untuk pengobatan diare, disentri, infeksi mulut, dan tenggorokan. Tanaman kedondong mengandung senyawa flavonoid, saponin, dan tannin yang merupakan senyawa aktif yang bersifatantibakteri. Tujuan penelitian untuk mengetahui daya hambat ekstrak daun kedondong (Spondias dulcis L.) terhadap Escerichia coli. Jenis penelitian yang dilakukan adalah eksperimen dengan ekstrak daun kedondong diberi perlakuan sebagai uji daya hambat terhadap pertumbuhan Escherichia coli. Ekstrak daun kedondong diperoleh dengan metode maserasi selama 5 hari dengan pelarut etanol. Pada penelitian ini menggunakan metode Kirby bauer disk diffusion (paper disk). Hasil penelitian menunjukkan bahwa terdapat zona bening disekitar paper disk yang menandakan ekstrak daun kedondong dapat menghambat pertumbuhan Escherichia coli. Ekstrak daun kedondong konsentrasi 5% memiliki zona hambat rata-rata 9,45 mm, konsentrasi 10% memiliki zona hambat rata-rata 11,65 mm, konsentrasi 15% memiliki zona hambat rata-rata 15,36 mm.Kata kunci: Zona Hambat, Daun Kedondong, Escherichia coli
ABSTRACT Kedondong is a tropical plant which is a family of Anacardiaceae. Kedondong plants are used as traditional medicine for the treatment of diarrhea, dysentery, mouth and throat infections. Kedondong plants contain flavonoid, saponin, and tannin compounds which are active antibacterial compounds. The aim of the study was to determine the inhibitory power of kedondong (Spondias dulcis L.) leaf extract against Escherichia coli. The type of research carried out was an experiment with kedondong leaf extract treated as an inhibitory test for the growth of Escherichia coli. Kedondong leaf extract was obtained by maceration method for 5 days with ethanol solvent. In this study using the Kirby bauer disk diffusion (paper disk) method. The results showed that there was a clear zone around the paper disk indicating that the extract of kedondong leaves could inhibit the growth of Escherichia coli. Kedondong leaf extract concentration of 5% has an average inhibition zone of 9.45 mm, a concentration of 10% has an average inhibition zone of 11.65 mm, a concentration of 15% has an average inhibition zone of 15.36 mm.
Keywords: Inhibitory Zone, Kedondong Leaves, Escherichia coli.
GAMBARAN GLUKOSA URINE DAN KETON URINE PASIEN HIPERTENSI DI RUMAH SAKIT UMUM DAERAH KOTA KENDARI
Authors: Susanti Susanti , Umar Ani , Trianti Ephi 
Published: June 2023

Hypertension is an increase in systolic blood pressure of at least 140 mmHg and a diastolic pressure of at least 90 mmHg. Urine is product of is end of system of metabolik released by kidney as remains or discard. Urine glucose is a simple sugar group that still in the urine after passing through various processes in the kidneys. Ketones is a metabolic product of fatty acids and proteins consisting of three compounds namely acetoacic acid, acetone, and beta hydroxybutyrate acid. Urine test can provide information about organabnormalities, but it can also be used to establish the result of treatment especially to monitor the development of disease such as high blood pressure (hypertension). Study aims to determine the description of glucose urine and ketone urine with patient hypertension. This type of research is descriptive by using device "study sectional cross" with sampling technique purposive sampling where the number of samples of 30 people. Based on the results of a study of 30 glucose urine and keton urine in patients hypertension at city general hospital kendari obtained normal urine glucose as many 5 patients (16,7%) and abnormal urine glucose 25 patients (83,3). While, ketone normal as many 30 patients (100%).
Keywords : Hypertension, Glucose Urine, Keton Urine
FORMULASI LOTION EKSTRAK DAUN Meistera chinensis SEBAGAI TABIR SURYA Meistera chinensis LEAF EXTRACT LOTION FORMULATION AS SUNSCREEN
Authors: Rusli Nirwati , Fauziah Yulianti , Yusdin Elviyanti 
Published: June 2023

Excessive sunlight can have a negative impact on skin health, but the bad effects of sunlight can be overcome by using sunscreen preparations. One of the natural ingredients that can be used in the manufacture of lotion sunscreenisleaf Meistera chinensis. Meistera chinensis is a plant that belongs to the Zingiberaceae family. Previous research has stated that Zingiberaceae species such as Etlingera elatior (torch ginger) have one of the functions as antioxidants. This study aims to formulateleaves Meistera chinensis as sunscreen and determine the SPF value obtained. Leaf extract was Meistera chinensis extracted using maceration method using methanol as solvent. Physical evaluation tests, stability tests, and sunscreen activity were carried out at three concentrations, namely 1% (F1), 3% (F2) and 5% (F3) concentrations. The three formulations are pale green, green, and dark green in color, have a melon flavor and have a liquid and semi-solid texture, are homogeneous with an oil-in-water (W/A) emulsion type and meet the pH requirements of 6 and 7. The stability test results show that the three formulas do not stable because it is not homogeneous and there is a change in aroma after the cycling test. The SPF value increased along with the increase in extract concentration, namely 7.46 (extra protection), 14.12 (maximum protection), and 19.06 (ultra protection).
Keywords: Extract, Leaf Meistera chinensis, Lotion, Sunscreen, SPF