bluuebunny/crossref_metadata_2025_split huggingface dataset:
Phase separation-based visualization of protein–protein interactions and kinase activities in plants
Authors: Safi Alaeddine , Smagghe Wouter , Gonçalves Amanda , Wang Qing , Xu Ke , Fernandez Ana Ibis , Cappe Benjamin , Riquet Franck B , Mylle Evelien , Eeckhout Dominique , De Winne Nancy , Van De Slijke Eveline , Persyn Freya , Persiau Geert , Van Damme Daniël , Geelen Danny , De Jaeger Geert , Beeckman Tom , Van Leene Jelle , Vanneste Steffen 
Published: June 2023

Abstract
Protein activities depend heavily on protein complex formation and dynamic posttranslational modifications, such as phosphorylation. The dynamic nature of protein complex formation and posttranslational modifications is notoriously difficult to monitor in planta at cellular resolution, often requiring extensive optimization. Here, we generated and exploited the SYnthetic Multivalency in PLants (SYMPL)-vector set to assay protein–protein interactions (PPIs) (separation of phases-based protein interaction reporter) and kinase activities (separation of phases-based activity reporter of kinase) in planta, based on phase separation. This technology enabled easy detection of inducible, binary and ternary PPIs among cytoplasmic and nuclear proteins in plant cells via a robust image-based readout. Moreover, we applied the SYMPL toolbox to develop an in vivo reporter for SNF1-related kinase 1 activity, allowing us to visualize tissue-specific, dynamic SnRK1 activity in stable transgenic Arabidopsis (Arabidopsis thaliana) plants. The SYMPL cloning toolbox provides a means to explore PPIs, phosphorylation, and other posttranslational modifications with unprecedented ease and sensitivity.
First Provider Seen for an Acute Episode of Low Back Pain Influences Subsequent Health Care Utilization
Authors: Bise Christopher G , Schneider Michael , Freburger Janet , Fitzgerald G Kelley , Switzer Galen , Smyda Garry , Peele Pamela , Delitto Anthony 
Published: June 2023

Abstract

Objective
Costs associated with low back pain (LBP) continue to rise. Despite numerous clinical practice guidelines, the evaluation and treatments for LBP are variable and largely depend on the individual provider. As yet, little attention has been given to the first choice of provider. Early research indicates that the choice of first provider and the timing of interventions for LBP appear to influence utilization. We sought to examine the association between the first provider seen and health care utilization.

Methods
Using 2015–2018 data from a large insurer, this retrospective analysis focused on patients (29,806) seeking care for a new episode of LBP. The study identified the first provider chosen and examined the following year of medical utilization. Cox proportional hazards models were calculated using inverse probability weighting on propensity scores to evaluate the time to event and the relationship to the first choice of provider.

Results
The primary outcome was the timing and use of health care resources. Total health care use was lowest in those who first sought care with chiropractic care or physical therapy. Highest health care use was seen in those patients who chose the emergency department.

Conclusion
Overall, there appears to be an association between the first choice of provider and future health care use. Chiropractic care and physical therapy provide nonpharmacologic and nonsurgical, guideline-based interventions. The use of physical therapists and chiropractors as entry points into the health system appears related to a decrease in immediate and long-term use of health resources. This study expands the existing body of literature and provides a compelling case for the influence of the first provider on an acute episode of LBP.

Impact
The first provider seen for an acute episode of LBP influences immediate treatment decisions, the trajectory of a specific patient episode, and future health care choices in the management of LBP.
China’s use of formal science and technology agreements as a tool of diplomacy
Authors: Wagner Caroline S , Simon Denis F 
Published: June 2023

Abstract
China’s government uses a variety of diplomatic tools to pursue its foreign policy aims including negotiating and signing formal bilateral science and technology agreements (STAs). These agreements have been signed with at least fifty-two countries. We identified agreements with an additional sixty-four countries with science and technology (S&T), among other topics such as education, as subjects for cooperation. The Ministry of Science and Technology reports having signed 115 intergovernmental science and technology agreements (STAs) and established ties with 161 countries and regions, although we were not able to identify all these agreements. The earliest of China’s STAs were signed in the 1950s with communist countries, but, in the late 1970s, China began signing agreements with scientifically-advanced nations, which opened opportunities for S&T cooperation. More recently, China has negotiated and signed scientific and technological cooperation agreements with dozens of middle- and lower-income countries, possibly to establish political goodwill. While building political ties clearly remains an important Chinese objective, access to the latest know-how in S&T has become a critical part of China’s priorities in establishing formal relationships.
IVChaucer
Authors: Menmuir Rebecca , Buchanan Peter , Kask Pamela 
Published: June 2023

Abstract
This chapter has four sections: 1. General; 2. The Canterbury Tales; 3. Other Works; 4. Reception. Section 1 is by Peter Buchanan and Pamela Kask; section 2 is by Rebecca Menmuir and Pamela Kask; section 3 is by Peter Buchanan; section 4 is by Rebecca Menmuir.
Recognition and Treatment of Wounds in Persons Using Xylazine: A Case Report From New Haven, Connecticut
Authors: Dowton Anne , Doernberg Molly , Heiman Erica , Barelli Peter , Golden Marjorie , Wang He , Leventhal Jonathan , Morford Kenneth L. , Sue Kimberly L. 
Published: June 2023

Background
Xylazine is an α2-adrenergic agonist that is commonly used as a veterinary tranquilizer and is increasingly present in the unregulated US drug supply since at least 2019. There are many suspected clinical complications of xylazine use, including unusual skin wounds, atypical overdose presentations, and possible dependence and withdrawal syndromes. However, there are few reports of cutaneous manifestations of xylazine in patients who inject drugs that can guide diagnosis and management in patients with confirmed xylazine toxicology.

Case Summary
We present the cases of 3 stably housed patients in Connecticut with opioid use disorder and intravenous use of fentanyl who presented with atypical, chronic wounds at the site of injection drug use. Xylazine toxicology sent on all 3 patients was positive. All patients were seen by wound care and dermatology, and 1 patient was followed by infectious diseases. Wound care management strategies are discussed as well as harm reduction strategies. For all patients, the dose of their medication for opioid use disorder was increased to decrease frequency of drug use given concern that patients were exposed to a drug supply containing xylazine.

Clinical Significance
This case report presents wound characteristics that raise the index of suspicion for xylazine-involved injection wounds and might assist in their diagnosis and management. There is urgent need for more reporting of such cases as well as rigorous research to understand the potential impact of xylazine on people who use drugs. Multidisciplinary best practices should be established.
Do Neuroprognostic Studies Account for Self-Fulfilling Prophecy Bias in Their Methodology? The SPIN Protocol for a Systematic Review
Authors: Teixeira Fernanda J. P. , Ahmad Bakhtawar , Gibatova Viktoriya , Ameli Pouya A. , Silva Ivan da , Carneiro Thiago , Roth William , Ford Jenna L. , Selfe Terry Kit , Greer David M. , Busl Katharina M. , Maciel Carolina B. 
Published: June 2023

Background:
Self-fulfilling prophecy bias occurs when a perceived prognosis leads to treatment decisions that inherently modify outcomes of a patient, and thus, overinflate the prediction performance of prognostic methods. The goal of this series of systematic reviews is to characterize the extent to which neuroprognostic studies account for the potential impact of self-fulfilling prophecy bias in their methodology by assessing their adequacy of disclosing factors relevant to this bias.

Methods:
Studies evaluating the prediction performance of neuroprognostic tools in cardiac arrest, malignant ischemic stroke, traumatic brain injury, subarachnoid hemorrhage, and spontaneous intracerebral hemorrhage will be identified through PubMed, Cochrane, and Embase database searches. Two reviewers blinded to each other’s assessment will perform screening and data extraction of included studies using Distiller SR and following Preferred Reporting Items for Systematic Reviews and Meta-Analyses guidelines. We will abstract data pertinent to the methodology of the studies relevant to self-fulfilling prophecy bias.

Results:
We will conduct a descriptive analysis of the data. We will summarize the reporting of mortality according to timing and mode of death, rates of exposure to withdrawal of life-sustaining therapy, reasoning behind limitations of supportive care, systematic use of standardized neuroprognostication algorithms and whether the tool being investigated is part of such assessments, and blinding of treatment team to results of neuroprognostic test being evaluated.

CONCLUSIONS:
We will identify if neuroprognostic studies have been transparent in their methodology to factors that affect the self-fulfilling prophecy bias. Our results will serve as the foundation for standardization of neuroprognostic study methodologies by refining the quality of the data derived from such studies.
Epidermal growth factor receptor compound and concomitant mutations: advances in precision treatment strategies
Authors: Li Wenqian , Bai Rilan , Guo Hanfei , Cui Jiuwei 
Published: June 2023

Abstract
Epidermal growth factor receptor (EGFR) mutations are common oncogenic driver mutations in patients with non-small cell lung cancer (NSCLC). The application of EGFR-tyrosine kinase inhibitors (TKIs) is beneficial for patients with advanced and early-stage NSCLC. With the development of next-generation sequencing technology, numerous patients have been found to have more than one genetic mutation in addition to a single EGFR mutation; however, the efficacy of conventional EGFR-TKIs and the optimal treatments for such patients remain largely unknown. Thus, we review the incidence, prognosis, and current treatment regimens of EGFR compound mutations and EGFR concomitant mutations to provide treatment recommendations and guidance for patients with these mutations.
Clinicopathologic Features of Noninvasive Inverted Urothelial Papillary Tumor
Authors: Liu Ziyu , Wei Shumei , Tang Jinlong 
Published: June 2023

Objectives:
Clinicopathologic features and recurrence rates of inverted noninvasive urothelial papillary tumors have been poorly characterized to date with few larger studies evaluating long-term outcomes. The spectrum of histomorphology, clinical features, and prognosis of inverted lesions of the urinary bladder are retrospectively reviewed.

Methods:
Archived paraffin-embedded urothelial tumor samples from patients diagnosed with inverted urothelial papillary lesions between January 2005 and June 2020 were collated. A matched control population of patients with exophytic papillary lesions of the urothelium diagnosed during the same time period was randomly selected. The conventional clinicopathologic features of inverted urothelial papillary tumor were evaluated retrospectively and patient demographics, tumor characteristics, recurrence, and survival information were recorded.

Results:
Lower recurrence rates were observed for inverted papillary urothelial neoplasm of low malignant potential (IPUNLMP) relative to papillary urothelial neoplasms of low malignant potential and for low-grade papillary urothelial carcinoma with an inverted growth pattern (LG-PUCI) relative to low-grade papillary urothelial carcinomas. No recurrence was found among the inverted urothelial papilloma cases. The 2- and 5-year disease-free survival rates were 100.0% and 85.2% for IPUNLMP patients; 94.4% and 80.4% for papillary urothelial neoplasms of low malignant potential; 89.5% and 82.0% for LG-PUCI; 73.7% and 54.6% for low-grade papillary urothelial carcinoma; 40.0% and 20.0% for high-grade papillary urothelial carcinoma with an inverted growth pattern patients and 26.7% and 26.7% for high-grade papillary urothelial carcinoma. Multivariate Cox regression analysis of IPUNLMP and LG-PUCI/high-grade papillary urothelial carcinoma with an inverted growth pattern indicated that tumor number (hazard ratio=4.356; 95% CI: 1.145-16.570; P=0.031) was a powerful prognostic factor for disease-free survival.

Conclusion:
Noninvasive, papillary urothelial lesions of the bladder tend to have lower recurrence and a better outcome if an inverted growth pattern is shown.
Don't Go Breaking My Heart: Not Your Ordinary Tattoo Reaction
Authors: McKenna Brian , Lee Jack , Raghavan Shyam , Zlotoff Barrett 
Published: June 2023

Abstract:
Necrobiosis lipoidica (NL) is a rare, chronic idiopathic granulomatous dermatitis with a somewhat controversial association with diabetes and other systemic diseases. We report a case of NL developing within a polychromic tattoo on the lower leg of a 53-year-old woman. Characteristic histopathologic findings of both active and chronic “burnt-out” NL appeared to originate from the tattoo where red ink was used 13 years prior. To the best of our knowledge, only 3 other cases of tattoo-associated NL have been reported.
Self-Healing Juvenile Cutaneous Mucinosis: A Report of Two Cases With Differing Evolution
Authors: Rodríguez-Cuadrado Francisco José , Reneses-Prieto Elvira , Silvestre-Egea Gala , López-López Agustín , Nájera-Botello Laura , Alfonso-Alberola Remedios , Moragón-Gordón Manuel , Roustan-Gullón Gaston , Onrubia-Pintado José , López-Negrete-Arenal Elena 
Published: June 2023

Abstract:
Self-healing juvenile cutaneous mucinosis is a rare entity, characterized by the presence of subcutaneous nodules together with frequent nonspecific systemic symptoms, which occurs in the pediatric age and characteristically resolves spontaneously. Although the diagnostic criteria do not require a biopsy to be performed, it is frequently performed, and an abundant dermal mucin deposition will be observed together with other features such as fibroblastic proliferation. Although the prognosis is benign, follow-up is required for the eventual development of a rheumatologic disease.
We present 2 clinical cases, describing the clinical findings and their histopathologic correlation. Comparatively, the outcome in both cases was different: in one case, the mucinosis resolved without any related event in the follow-up, and in the other case, the resolution was accompanied by the subsequent development of idiopathic juvenile arthritis.
Rhomboid intercostal block or thoracic paravertebral block for postoperative recovery quality after video-assisted thoracic surgery
Authors: Wang Xuru , Jia Xiaoyu , Li Zhenping , Zhou Qinghe 
Published: June 2023

BACKGROUND
The analgesic characteristics of rhomboid intercostal block (RIB) remain unclear. Before it can be fully recommended, we compared the recovery quality and analgesic effects of RIB and thoracic paravertebral block (TPVB) for video-assisted thoracoscopic surgery (VATS).

OBJECTIVE
The current study aimed to investigate whether there is a difference in postoperative recovery quality between TPVB and RIB.

DESIGN
A prospective, non-inferiority, randomised controlled trial.

SETTING
Affiliated Hospital of Jiaxing University in China from March 2021 to August 2022.

PATIENTS
Eighty patients aged 18 to 80 years, with ASA physical status I to III, and scheduled for elective VATS were enrolled in the trial.

INTERVENTION
Ultrasound-guided TPVB or RIB was performed with 20 ml 0.375% ropivacaine.

MAIN OUTCOME MEASURES
The primary outcome of the study was the mean difference of quality of recovery-40 scores 24 h postoperatively. The non-inferiority margin was defined as 6.3. Numeric rating scores (NRS) for pain at 0.5, 1, 3, 6, 12, 24 and 48 h postoperatively in all patients were also recorded.

RESULTS
A total of 75 participants completed the study. The mean difference of quality of recovery-40 scores 24 h postoperatively was −1.6 (95% CI, −4.5 to 1.3), demonstrating the non-inferiority of RIB to TPVB. There was no significant difference between the two groups in the area under the curve for pain NRS over time, at rest and on movement, at 6, 12, 24 and 48 h postoperatively (all P > 0.05), except for the area under the curve pain NRS over time on movement at 48 h postoperatively (P = 0.046). There were no statistical differences between the two groups in the postoperative sufentanil use at 0 to 24 h or 24 to 48 h (all P > 0.05).

CONCLUSION
Our study suggests that RIB was non-inferior to TPVB for the quality of recovery, with almost the same postoperative analgesic effect as TPVB after VATS.

CLINICAL TRIAL REGISTRATION
chictr.org.cn: ChiCTR2100043841.
Elevated FBXL18 promotes RPS15A ubiquitination and SMAD3 activation to drive HCC
Authors: Yu Hong-Qiang , Li Feng , Xiong HaoJun , Fang Lei , Zhang Jie , Bie Ping , Xie Chuan-Ming 
Published: June 2023

Background:
F-box and leucine-rich repeat protein 18 (FBXL18) is an E3 ubiquitin ligase that is reported to be involved in the tumorigenesis of various types of cancer. However, it remains unknown whether FBXL18 is correlated with hepatocarcinogenesis.

Methods and results:
In the current study, we found that FBXL18 was highly expressed in HCC tissues and positively associated with poor overall survival of HCC patients. FBXL18 was an independent risk factor for HCC patients. We observed that FBXL18 drove HCC in FBXL18 transgenic mice. Mechanistically, FBXL18 promoted the K63-linked ubiquitination of small-subunit ribosomal protein S15A (RPS15A) and enhanced its stability, increasing SMAD family member 3 (SMAD3) levels and translocation to the nucleus and promoting HCC cell proliferation. Moreover, the knockdown of RPS15A or SMAD3 significantly suppressed FBXL18-mediated HCC proliferation. In clinical samples, elevated FBXL18 expression was positively associated with RPS15A expression.

Conclusion:
FBXL18 promotes RPS15A ubiquitination and upregulates SMAD3 expression, leading to hepatocellular carcinogenesis, and this study provides a novel therapeutic strategy for HCC treatment by targeting the FBXL18/RPS15A/SMAD3 pathway.
Comparison of implantation of posterior chamber phakic IOL implantation and laser vision correction in terms of corneal endothelial cells: 3-year observational paired-eye study
Authors: Choi Hannuy , Ryu Ik Hee , Lee In Sik , Kim Jin Kuk , Yoo Tae Keun 
Published: June 2023

Purpose:
To compare the postoperative endothelial cell counts of EVO-implantable collamer lenses (ICLs) with a central hole (V4c and V5) and laser vision correction surgery (laser in situ keratomileusis or photorefractive keratectomy).

Setting:
B&VIIT Eye Center, Seoul, South Korea.

Design:
Retrospective observational and paired contralateral study.

Methods:
62 eyes of 31 patients who underwent EVO-ICLs with a central hole implantation in one eye (phakic intraocular lens [pIOL] group) and laser vision correction in the contralateral eye (LVC group) to correct refractive errors were retrospectively reviewed. Central endothelial cell density (ECD), percentage of hexagonal cells (HEX), coefficient of variation (CoV) in cell size, and adverse events were evaluated for at least 3 years. The endothelial cells were observed using a noncontact specular microscope.

Results:
All surgeries were performed, without complications during the follow-up period. The mean ECD loss values compared with the preoperative measurements were 6.65% and 4.95% during the 3 years after pIOL and LVC, respectively. There was no significant difference in ECD loss compared with the preoperative values (paired t test, P = .188) between the 2 groups. No significant loss in ECD was observed at any timepoint. The pIOL group showed higher HEX (P = .018) and lower CoV (P = .006) values than the LVC group at the last visit.

Conclusions:
According to the authors' experience, the EVO-ICL with a central hole implantation was a safe and stable vision correction method. Moreover, it did not induce statistically significant changes in ECD at 3 years postoperatively compared with LVC. However, further long-term follow-up studies are required to confirm these results.
Prevalence and Correlates of Cardiovascular, Pulmonary, Cancer, and Mental Health Comorbidities Among Adults With HIV Who Smoke
Authors: Elf Jessica L. , Horn Kimberly , Abroms Lorien , Stanton Cassandra A. , Cohn Amy M. , Spielberg Freya , Gray Tiffany , Harvey Emily , Debnam Charles , Kierstead Lexi , Levy Matthew E. , Castel Amanda , Monroe Anne , Niaura Raymond 
Published: June 2023

Abstract
Using data from the D.C. Cohort Longitudinal HIV Study, we examined (a) diagnosed mental health and (b) cardiovascular, pulmonary, or cancer (CPC) comorbidity among adults with HIV who smoked. Among 8,581 adults, 4,273 (50%) smoked; 49% of smokers had mental health, and 13% of smokers had a CPC comorbidity. Among smokers, non-Hispanic Black participants had a lower risk for mental health (prevalence ratio [PR]: 0.69; 95% confidence interval [CI] [0.62–0.76]) but a higher risk for CPC (PR: 1.17; 95% CI [0.84–1.62]) comorbidity. Male participants had a lower risk for mental health (PR: 0.88; 95% CI [0.81–0.94]) and CPC (PR: 0.68; 95% CI [0.57–0.81]) comorbidity. All metrics of socioeconomic status were associated with a mental health comorbidity, but only housing status was associated with a CPC comorbidity. We did not find any association with substance use. Gender, socioeconomic factors, and race/ethnicity should inform clinical care and the development of smoking cessation strategies for this population.