bluuebunny/crossref_metadata_2025_split huggingface dataset:
Compensation or preservation? Different roles of functional lateralization in speech perception in older non-musicians and musicians
Authors: Jin Xinhu , Zhang Lei , Wu Guowei , Wang Xiuyi , Du Yi 
Published: April 2023

AbstractMusical training can offset age-related decline of speech perception in noisy environments. However, whether functional compensation or functional preservation the older musicians adopt to counteract the adverse effects of aging is unclear yet, so do older non-musicians. Here, we employed the fundamental brain organization feature named functional lateralization, and calculated network-based lateralization indices (LIs) of resting-state functional connectivity (FC) in 23 older musicians (OM), 23 older non-musicians (ONM), and 24 young non-musicians (YNM). OM outperformed ONM and almost equalized YNM in speech-in-noise/speech tasks. In parallel, ONM exhibited reduced lateralization than YNM in LI of intrahemispheric FC (LI_intra) in cingulo-opercular network (CON) and interhemispheric heterotopic FC (LI_he) in language network (LAN). Moreover, OM showed higher neural alignment to YNM (i.e., similar lateralization pattern) than ONM in LI_intra in CON, LAN, frontoparietal network (FPN) and default mode network (DMN) and LI_he in DMN. These findings suggest that musical training contributes to the preservation of youth-like lateralization in older adults. Furthermore, stronger left-lateralized and lower alignment-to-young of LI_intra in somatomotor network (SMN) and dorsal attention network (DAN) and LI_he in DMN correlated with better speech performance in ONM. In contrast, stronger right-lateralized LI_intra in FPN and DAN and higher alignment-to-young of LI_he in LAN correlated with better performance in OM. Thus, functional preservation and compensation of lateralization may play different roles in speech perception in noise for the elderly with and without musical expertise, respectively. Our findings provide insight into successful aging theories from the unique perspective of functional lateralization and speech perception.Significance statementAs a positive lifestyle which contributes to neural resource enrichment, musical training experience may mitigate age-related decline in speech perception in noise through both functional compensation and preservation. What is unknown is whether older musicians rely more on one of these mechanisms, and how is it different from older non-musicians. From a unique perspective of functional lateralization, we found that high-performing older musicians showed stronger preservation of youth-like lateralization with a more right-lateralized pattern whereas high-performing older non-musicians were associated with stronger scaffolding of compensatory networks with a more left-lateralized pattern. Our findings suggest that older musicians and non-musicians exhibit different coping strategies in terms of functional lateralization against aging, which would largely enrich aging theories and inspire training intervention.
Antivirals against monkeypox infections
Authors: Chiem Kevin , Nogales Aitor , Lorenzo Maria , Vasquez Desarey Morales , Xiang Yan , Gupta Yogesh K. , Blasco Rafael , de la Torre Juan Carlos , Martínez-Sobrido Luis 
Published: April 2023

ABSTRACTMonkeypox virus (MPXV) infection in humans are historically restricted to endemic regions in Africa. However, in 2022, an alarming number of MPXV cases have been reported globally with evidence of person-to-person transmission. Because of this, the World Health Organization (WHO) declared the MPXV outbreak a public health emergency of international concern. MPXV vaccines are limited and only two antivirals, tecovirimat and brincidofovir, approved by the United States (US) Food and Drug Administration (FDA) for the treatment of smallpox, are currently available for the treatment of MPXV infection. Here, we evaluated 19 compounds previously shown to inhibit different RNA viruses for their ability to inhibit Orthopoxvirus infections. We first used recombinant vaccinia virus (rVACV) expressing fluorescence (Scarlet or GFP) and luciferase (Nluc) reporter genes to identify compounds with anti-Orthopoxvirus activity. Seven compounds from the ReFRAME library (antimycin A, mycophenolic acid, AVN- 944, pyrazofurin, mycophenolate mofetil, azaribine, and brequinar) and six compounds from the NPC library (buparvaquone, valinomycin, narasin, monensin, rotenone, and mubritinib) showed antiviral activity against rVACV. Notably, the anti-VACV activity of some of the compounds in the ReFRAME library (antimycin A, mycophenolic acid, AVN- 944, mycophenolate mofetil, and brequinar) and all the compounds from the NPC library (buparvaquone, valinomycin, narasin, monensin, rotenone, and mubritinib) were confirmed with MPXV, demonstrating the broad-spectrum antiviral activity against Orthopoxviruses and their potential to be used for the antiviral treatment of MPXV, or other Orthopoxvirus, infections.IMPORTANCEDespite the eradication of smallpox, some Orthopoxviruses remain important human pathogens, as exemplified by the recent 2022 monkeypox virus (MPXV) outbreak. Although smallpox vaccines are effective against MPXV, there is presently limited access to those vaccines. In addition, current antiviral treatment against MPXV infections is limited to the use of the FDA-approved drugs tecovirimat and brincidofovir. Thus, there is an urgent need to identify novel antivirals for the treatment of MPXV, and other potentially zoonotic Orthopoxvirus infections. Here, we show that thirteen compounds, derived from two different libraries, previously found to inhibit several RNA viruses, exhibit also antiviral activity against VACV. Notably, eleven compounds also displayed antiviral activity against MPXV, demonstrating their potential to be incorporated into the therapeutic armamentarium to combat Orthopoxvirus infections.
Fetal manipulation of maternal metabolism is a critical function ofIgf2imprinting
Authors: Lopez-Tello Jorge , Yong Hannah E. J. , Sandovici Ionel , Christoforou Efthimia , Salazar-Petres Esteban , Boyland Rebecca , Napso Tina , Constancia Miguel , Sferruzzi-Perri Amanda N. 
Published: April 2023

AbstractMaternal-offspring interactions in mammals are mainly characterised by cooperation, but also conflict. Over evolutionary time, the fetus has evolved to manipulate the mother’s physiology to increase nutrient transfer through the placenta, but these mechanisms are poorly characterized. The imprintedIgf2(insulin-like growth factor 2) gene is highly expressed in mouse placental cells with endocrine functions. Here, we show that in the mouse, deletion ofIgf2in these cells leads to impaired placental endocrine signalling to the mother, but remarkably does not result in changes in placental morphology, growth or size. Mechanistically, we find thatIgf2via defective production of hormones, including prolactins, is essential for the establishment of the insulin-resistance state during pregnancy, and the appropriate partitioning of nutrients to the developing fetus. Consequently, fetuses are growth restricted and hypoglycemic, due to impaired placental glucose transfer from the mother to the fetus. Furthermore,Igf2loss from placental endocrine cells has long-lasting effects on offspring adiposity and glucose homeostasis in adult life. Our study provides long-sought compelling experimental evidence for an intrinsic fetal manipulation system, which operates in the placenta to modify maternal metabolism and resource allocation to the fetus, with consequences for offspring metabolic health in later life.
Imaging-based screening identifies modulators of theeIF3translation initiation factor complex inCandida albicans
Authors: Metzner Katura , O’Meara Matthew J , Halligan Benjamin , Wotring Jesse W. , Sexton Jonathan Z , O’Meara Teresa R 
Published: April 2023

AbstractFungal pathogens likeCandida albicanscan cause devastating human disease. Treatment of candidemia is complicated by the high rate of resistance to common antifungal therapies. Additionally, there is host toxicity associated with many antifungal compounds due to the conservation between essential mammalian and fungal proteins. An attractive new approach for antimicrobial development is to target virulence factors: non-essential processes that are required for the organism to cause disease in human hosts. This approach expands the potential target space while reducing the selective pressure towards resistance, as these targets are not essential for viability. InC. albicans,a key virulence factor is the ability to transition to hyphal morphology. We developed a high-throughput image analysis pipeline to distinguish between yeast and filamentous growth inC. albicansat the single cell level. Based on this phenotypic assay, we screened the FDA drug repurposing library of 2,017 compounds for their ability to inhibit filamentation and identified 33 compounds that block the hyphal transition inC. albicanswith IC50values ranging from 0.2 to 150 µM. Multiple compounds showed a phenyl vinyl sulfone chemotype, prompting further analysis. Of these phenyl vinyl sulfones, NSC 697923 displayed the most efficacy, and by selecting for resistant mutants, we identifiedeIF3as the target of NSC 697923 inC. albicans.
Resin acids play key roles in shaping microbial communities during degradation of spruce bark
Authors: Ristinmaa Amanda Sörensen , Rangel Albert Tafur , Idström Alexander , Valenzuela Sebastian , Kerkhoven Eduard J. , Pope Phillip B. , Hasani Merima , Larsbrink Johan 
Published: April 2023

AbstractThe bark is the outermost defense of trees against microbial attack, largely thanks to toxicity and prevalence of extractive compounds. Nevertheless, bark decomposes in nature, though by which species and mechanisms remains unknown. Here, we have followed the development of microbial enrichments growing on spruce bark over six months, by monitoring both chemical changes in the material and performing community and metagenomic analyses. Carbohydrate metabolism was unexpectedly limited, and instead a key activity was metabolism of extractives. Resin acid degradation was principally linked to community diversification with specific bacteria revealed to dominate the process. Metagenome-guided isolation facilitated the recovery of the dominant enrichment strain in pure culture, which represents a new species (Pseudomonas abieticivoranssp. nov.), that can grow on resin acids as a sole carbon source. Our results illuminate key stages in degradation of an abundant renewable resource, and how defensive extractive compounds have major roles in shaping microbiomes.
Uncovering the heterogeneity of pancreatic endothelial cells using integrative and comparative single cell gene expression analysis
Authors: Khan Safwat T. , Ahuja Neha , Taib Sonia , Vohra Shabana , Cleaver Ondine , Nunes Sara S 
Published: April 2023

AbstractThe pancreatic islet vasculature displays tissue-specific physiological and functional adaptations that support rapid glucose sensing and insulin response by β-cells. To uncover the transcriptomic basis of this specialization, we performed a meta-analysis of multi-organ single cell RNA sequencing atlases employing a unique strategy to avoid transcriptomic contamination. We identified biologically relevant genes involved in sphingosine-1-phosphate-mediated insulin-secretion (PLPP1, RDX, CDC42EP1), islet basement membrane formation (SPARC, COL15A1), endothelial cell (EC) permeability (PLVAP, EHD4), membrane transporters (CD320, SLCO2A1)and developmental transcription factors (NKX2-3, AHR). These were validatedin silicoin independent datasets. We further established the first integrated transcriptomic atlas of human pancreatic ECs and described two unique capillary subpopulations: exocrine and endocrine pancreas ECs. We validated the spatial localization of key markers using RNAscope™ and immunofluorescence staining on mouse pancreatic tissue cross-sections. Our findings provide novel insights into pancreatic EC heterogeneity and islet EC function with potential implications in therapeutic strategies.
Melatonin alleviates LPS-induced abnormal pregnancy through MTNR1B regulation of m6A
Authors: Zhao Shisu , Dong Yanjun , Li Yuanyuan , Wang Zixu , Chen Yaoxing , Dong Yulan 
Published: April 2023

AbstractPregnancy is a very complex and delicate process, inflammation in early pregnancy may result in pregnancy loss or defective implantation. Melatonin, mainly produced from the pineal body, which exerts several pharmacological effects. N6-methyladenosine (m6A) is the most prevalent modification of eukaryotic mRNA. The aim of this study was to investigate the association between melatonin and m6A during pregnancy and elaborate the underlying protective mechanism of melatonin during pregnancy. In vitro, melatonin was found to alleviated LPS-induced reductions in the number of implantation sites. Besides, melatonin was found to alleviate the activation of inflammation, autophagy and apoptosis pathways. In vitro studies demonstrated that melatonin regulated several downstream pathways in an m6A-dependent manner via melatonin receptor MTNR1B. Our findings revealed the important roles of m6A in the establishment of pregnancy and discovered a new mechanism of how melatonin protects pregnancy.
Conserved enhancer logic controls the notochord expression of vertebrateBrachyury
Authors: Kemmler Cassie L. , Smolikova Jana , Moran Hannah R. , Mannion Brandon J. , Knapp Dunja , Lim Fabian , Czarkwiani Anna , Aguayo Viviana Hermosilla , Rapp Vincent , Fitch Olivia E. , Bötschi Seraina , Selleri Licia , Farley Emma , Braasch Ingo , Yun Maximina , Visel Axel , Osterwalder Marco , Mosimann Christian , Kozmik Zbynek , Burger Alexa 
Published: April 2023

ABSTRACTThe cell type-specific expression of key transcription factors is central to development. Brachyury/T/TBXT is a major transcription factor for gastrulation, tailbud patterning, and notochord formation; however, how its expression is controlled in the mammalian notochord has remained elusive. Here, we identify the complement of notochord-specific enhancers in the mammalianBrachyury/T/TBXTgene. Using transgenic assays in zebrafish, axolotl, and mouse, we discover threeBrachyury-controlling notochord enhancersT3, C, andIin human, mouse, and marsupial genomes. Acting as Brachyury-responsive, auto-regulatory shadow enhancers, deletion of all three enhancers in mouse abolishes Brachyury/T expression selectively in the notochord, causing specific trunk and neural tube defects without gastrulation or tailbud defects. Sequence and functional conservation ofBrachyury-driving notochord enhancers with thebrachyury/tbxtbloci from diverse lineages of fishes dates their origin to the last common ancestor of jawed vertebrates. Our data define the enhancers forBrachyury/T/TBXTBnotochord expression as ancient mechanism in axis development.
Escaping transparent, asymmetrical and agentive accountability? Entrepreneurial accounts and researchers' countertransference
Authors: Besson Madeleine , Jacquinot Philippe , Jardat Rémi , Moriceau Jean-Luc 
Published: April 2023

PurposeThis article of exploratory research provides a critical perspective on accountability, focusing on three characteristics: transparency, asymmetry and individual agency. An experimental method is developed, calling for an ethics of accountability.Design/methodology/approachFour entrepreneurs have given accounts of themselves and their projects in life cycle interviews. This article applies Devereux's approach (1967), which allows for opacity (the “unconscious”) to oneself and to others with symmetry between analysts and analysed, and a lack of demarcation between the observer and the observed.FindingsA tragic entrepreneurial accountability trap of continuous self-justification was discovered, which pertains both to the entrepreneurs and the researchers. Nonetheless, the researchers as inspired by Devereux's method were able to realize a form of accounterability.Social implicationsThis article shows that the demands for transparent, asymmetrical and agentive accountability call for ethical reflection. The request for accounts, as resulting in the accounts given and the research conducted into accountability, are all sources of constraints. Differing the accountability situation may lessen the constraints.Originality/valueThis study introduces Devereux's method as an investigative tool in accountability research, opening up new perspectives on communication and analysis. This article shows the researcher as situated both inside and outside of the accountability mechanisms. This article explores a singular form of accountability; that of entrepreneurs who seemingly only account for the future, thereby disconnecting them from others.
The well-being of female administrative staff in managerial positions in Polish Higher Education Institutions
Authors: Górak-Sosnowska Katarzyna , Piwowar-Sulej Katarzyna 
Published: April 2023

PurposeThe aim of the paper is to theoretically and empirically explore the issue of well-being (WB) of female administrative employees who work on managerial positions at higher education institutions (HEIs).Design/methodology/approachThis study is based on both literature studies and explorative empirical research conducted in Poland with the use of snowball sampling. It adopted a questionnaire authored by Parker and Hyett and covered 121 respondents.FindingsLiterature studies show that invisibility of work, low level of empowerment, increased stress, workload and expectations, reduced resources, high level of anxiety, fatigue and low level of vitality negatively impact the WB of HEIs’ administrative staff. The presented research provides insight into the internal structure of the administrative staff’s WB. Both the main construct (i.e. employees’ WB) and its subconstructs are on moderate level. Respondents’ age and tasks performed are correlated only with the subconstruct of WB in the form of intrusion of work into private life.Research limitations/implicationsAlthough the research is not based on large sample, it provides both practical and theoretical implications.Originality/valueMost of studies discuss the issue of WB of scholars. The paper fills the research gap in terms of examining female administrative employees who work on managerial positions at HEIs.
Digital transformation and strategic agility during the COVID-19 crisis: the role of the intangible capital conversion
Authors: Bounfour Ahmed , Housel Thomas , Silkey Trent , Nonnis Alberto 
Published: April 2023

PurposeThe purpose of the current study is to illustrate the importance of strategic agility (SA), the capacity to respond agilely to a rapidly changing environment, for digitally transforming firms during the COVID-19 crisis. A secondary purpose of the study is to conceptually frame SA as a function of the creative to realized intangible capital (IC) ratio.Design/methodology/approachTo inferentially corroborate the hypothesis, this study exploits the results of a recent firm-level survey, conducted under the H2020 project GlobalInto (2021). Via OLS and ordered logistic regressions, the relationship among SA, economic performance and IC was tested.FindingsThe exploratory findings implied that the more strategically agile companies were those that responded more effectively to the pandemic crisis, but only if they were ahead in terms of digital transformation. Moreover, the results implied that firms that were able to efficiently convert their creative IC into realized IC were the most strategically agile.Originality/valueThis study developed a new conceptual framework for digitally transforming firms that included the role of SA and the IC conversion ratio in the context of extreme threats to the survival of firms. Some preliminary practical recommendations were offered to management about how to measure the IC conversion ratio as well as how to stimulate and reward greater creativity among employees, filling a notable gap in the SA literature that provides less than precise guidance about how this concept can be measured.
Predicting the effect of different magnetic fields on the solute segregation during direct chill casting of large-size magnesium alloy slab
Authors: Duan Wenchao , Yang Yiqiang , Liu Wenhong , Zhang Zhiqiang , Cui Jianzhong 
Published: April 2023

Purpose
The purpose of this paper is to reveal the solute segregation behavior in the molten and solidified regions during direct chill (DC) casting of a large-size magnesium alloy slab under no magnetic field (NMF), harmonic magnetic field (HMF), pulsed magnetic field (PMF) and two types of out-of-phase pulsed magnetic field (OPMF).

Design/methodology/approach
A 3-D multiphysical coupling mathematical model is used to evaluate the corresponding physical fields. The coupling issue is addressed using the method of separating step and result inheritance.

Findings
The results suggest that the solute deficiency tends to occur in the central part, while the solute-enriched area appears near the fillet in the molten and solidified regions. Applying magnetic field could greatly homogenize the solute field in the two-phase region. The variance of relative segregation level in the solidified cross-section under NMF is 38.1%, while it is 21.9%, 18.6%, 16.4% and 12.4% under OPMF2 (the current phase in the upper coil is ahead of the lower coil), HMF, PMF and OPMF1 (the current phase in the upper coil lags behind the lower coil), respectively, indicating that OPMF1 is more effective to reduce the macrosegregation level.

Originality/value
There are few reports on the solute segregation degree in rectangle slab under magnetic field, especially for magnesium alloy slab. This paper can act a reference to make clear the solute transport behavior and help reduce the macrosegregation level during DC casting.
Interactive CSR campaign and symbolic brand benefits: a moderated mediation model of brand trust and self-congruity in the restaurant industry
Authors: Sung Kyong Sik , Lee Seoki 
Published: April 2023

Purpose
Drawing on symbolic interaction theory (SIT), this study aims to identify what makes corporate social responsibility (CSR) communication more favorable to customers in the chain restaurants context. Specifically, this study examines the direct relationships between the interactivity of CSR communication, brand trust and brand sincerity. In addition, the mediating role of brand trust (i.e. separate dimensions of brand reliability and intentions) and the moderated mediating role of self-congruity are explored.

Design/methodology/approach
A total of 418 US consumers with past experiences of participating in CSR campaigns organized by chain restaurants on social media were recruited using the online survey method of nonprobability sampling through Amazon Mechanical Turk in December 2021.

Findings
The results of this study revealed that the interactivity of CSR communication on social media affects brand sincerity; brand reliability and brand intentions mediate the positive effect of interactivity of CSR communication on brand sincerity; and customer’s self-congruity moderated the positive mediation effect via brand reliability.

Practical implications
Chain restaurant marketers need to understand the important role of interactivity as a key element of CSR communication on social media to help develop brand trust and brand sincerity in chain restaurants.

Originality/value
This study expands on SIT to support the symbolic benefits of interactive CSR communication on social media.