bluuebunny/crossref_metadata_2025_split huggingface dataset:
Quality analysis of YouTube videos on vulvodynia
Authors: Aglamis Savas Ozgur , Senel Samet , Koudonas Antonios 
Published: January 2023

AbstractBackgroundSocial media, with its low cost and easy accessibility, represents an ideal medium for sharing medical interests, but the quality of its content is questionable.AimThe primary aim of this study was to evaluate the quality of video content related to vaginismus on YouTube as a source of information by using scores of established classification systems. The secondary aim was to examine the relationship between objective and subjective measures of their quality.MethodsThe term vaginismus was typed into the YouTube search bar (http://www.youtube.com). The first 50 videos with the highest number of views were included in the study. All videos were assessed on August 18, 2022, by a gynecologist and/or a urologist experienced in vulvodynia. Data of all the videos were recorded, such as source, content, duration, day since upload date, number of views, number of likes, number of comments, and views per day. The Global Quality Scale (GQS) and modified DISCERN score were employed to assess the quality of the videos.OutcomesThe primary outcomes of this study were the scores of established classification systems and the measures relating to the preference and evaluation of viewers of YouTube videos on vulvodynia.ResultsA total of 50 videos were evaluated. The sources of 32 (64%) of these videos were “universities/professional organizations/nonprofit physicians/physicians” and “stand-alone health information websites.” The GQS and modified DISCERN scores of the videos whose source was “universities/professional organizations/nonprofit physicians/physicians” were higher than those whose source was “talk show programs/television programs” (P = .014 for GQS score, P = .046 for modified DISCERN score). When classified according to GQS score, the quality level of 58% of the videos was low. Of the videos whose source was “universities/professional organizations/nonprofit physicians/physicians,” 56.3% were of good quality.Clinical ImplicationsThe quality of information was very low and suggested that health care professionals should take on a more active role in configuring the qualitative characteristics of the material available online about the given health issues.Strengths and LimitationsTo the best of our knowledge, this is the first study to investigate the quality of YouTube videos on vaginismus (vulvodynia). However, the limitation of this study is the relatively subjective evaluation of videos, including the risk of observer bias, although we attempted to overcome this problem with the inclusion of 2 independent reviewers and the use of validated tools.ConclusionYouTube videos may offer a huge amount of information about this condition, but the quality of the available sources is heterogeneous.
First insights into the current practice, knowledge, and attitudes of community pharmacists regarding sexual adverse drug reactions: a cross-sectional survey
Authors: Gordijn Rineke , Nicolai Melianthe P J , Elzevier Henk W , Guchelaar Henk-Jan , Teichert Martina 
Published: January 2023

AbstractIntroductionSexual function can be negatively influenced by adverse drug reactions (ADRs) potentially caused by &gt;300 drugs. These sexual ADRs (sADRs) can lead to low adherence and decreased quality of life. Physicians are known to barely discuss sexual function. Pharmacists also have an important role in informing and advising patients on ADRs, but it is unknown how community pharmacists deal with sADRs.AimsThe purpose of this study was to evaluate the current practice, attitudes, and knowledge of community pharmacists about informing, detecting, and discussing sADRs.MethodsAn online survey with 31 questions was sent to all 1932 pharmacy members of the Royal Dutch Pharmacists Association. The survey was modified from previous surveys that questioned different medical disciplines on their practice, attitudes, and knowledge of sexual function related to their fields. Questions were added on pharmacists’ practice concerning ADRs in general.ResultsA total of 97 (5%) pharmacists responded. During first dispenses of drugs, 64 (66%) informed patients on a selection of common ADRs. Almost all (n = 93, 97%) discussed diarrhea or constipation in at least half of the related occasions, whereas 26 to 31 (27%-33%) discussed sADRs. The sADRs for high-risk drugs were more often named at first than at second dispenses (n = 61 [71%] vs n = 28 [32%]). Pharmacy technicians were generally considered not to discuss sADRs (n = 73, 76%; never or in less than half of the occasions). Lack of privacy (n = 54, 57%) and language barriers (n = 45, 47%) were the most acknowledged barriers to discuss sADRs. Moreover, 46% (n = 45) considered their knowledge insufficient to discuss sADRs. Responsibility for informing, advising, and detecting sADRs was most often attributed to pharmacy technicians (n = 59, 62%), pharmacists (n = 46, 48%), and patients (n = 75, 80%), respectively.ConclusionThis study shows that one-third of pharmacists and two-thirds of pharmacy technicians barely talked about sADRs during first dispenses for high-risk drugs. The low response rate suggests that mostly interested pharmacists responded, thus likely overestimating the sADR discussion rate. To provide patients with unique opportunities to discuss sADRs in community pharmacies, more attention is needed for raising awareness about the topic among pharmacists and for barriers such as the presence of other clients and limited knowledge about sADRs.
Resistance of Musa balbisiana Accessions of the Philippines to Banana Bunchy Top Virus
Authors: Dela Cueva Fe M. , Perez Nicole Angelee M. , Benjamin Ann Fatima A. , Yanos Lyka A. , Gueco Lavernee S. , Thomas John E. 
Published: January 2023

Banana bunchy top disease (BBTD) is caused by banana bunchy top virus (BBTV), the most important virus affecting banana. Currently, no cultivar or accession of banana has complete resistance to BBTD. A total of 36 wild Musa spp. accessions, including 34 Musa balbisiana and 2 M. acuminata subsp. errans (“Agutay”), were screened for resistance against BBTV. In greenhouse tests using viruliferous banana aphids (Pentalonia nigronervosa), all M. balbisiana accessions remained symptomless, and BBTV was not detected in any of these plants by PCR at 3 and 6 months postinoculation. In contrast, 100% disease incidence was recorded in M. acuminata subsp. errans and in cv. Lakatan susceptible control plants. The PCR-negative M. balbisiana plants were then transferred to a field with high BBTV inoculum pressure where they remained symptomless and PCR-negative for up to 5 years, while all cv. Lakatan developed BBTD. Wild M. balbisiana accessions showed a high level of resistance and possibly immunity to BBTV and are expected to provide a resource for conventional and marker-assisted breeding. [Formula: see text] Copyright © 2023 The Author(s). This is an open access article distributed under the CC BY-NC-ND 4.0 International license .
S-06-4: IMPACT OF ANTI-HYPERTENSIVE THERAPY ON CEREBRAL FUNCTIONAL NETWORKS AND CONNECTIVITY
Authors: Carnevale Lorenzo , Maffei Angelo , Landolfi Alessandro , Grillea Giovanni , Berardi Valeria , Carnevale Daniela , Lembo Giuseppe 
Published: January 2023

It is well known that hypertension is the main modifiable risk factor leading to cognitive impairment and various forms of dementia. Leveraging functional MRI it has been possible to characterize hypertensives brain functional connectivity, a main feature of cerebral activation and health measurable by resting-state functional MRI. This characterization let us analyze how pathological conditions influence the cognitive processes and the spontaneous organization of brain activity, and have shown that hypertensive patients even before showing signs of vascular dementia have alterations in cerebral connections compared to normotensive patients. Our current question is whether different classes of antihypertensives, differentiated in mechanism of action and potential to cross the blood brain barrier may have different impact on cerebral functional organization.
42 hypertensives with controlled hypertension (SBP< 140mmHg e DBP< 90mmHg) were subjected to resting-state functional MRI. Functional connectivity was elaborated by CONN, according to an analysis pipeline to build functional connectivity networks matrices calculating the cross-correlation of BOLD signal among different brain regions. The obtained network of connections has been analyzed by general linear model, to implement regression models that highlight the connections in the brains modulated by the specific classes of antihypertensives analyzed.
The application of regression models with age, sex, BMI, alcohol, smoke, SBP and DBP levels, heart rate as covariates shown that treatment with diuretics modulate a large connectivity network among Salience Network, Default Mode Network and Language Network (Fig. A). Ace-inhibitors treatment is associated with a significant modulation of a connection between Default Mode Network and the FrontoParietal Network (Fig. B), while ARBs are associated to the modulation of connections between Cerebellar and Language Networks (Fig. C). Finally, beta-blockers are associated with connections centered in Dorsal Attention Network and projected to Language and FrontoParietal Networks (Fig. D).
Globally, the most relevant functional modulation of the brain is imposed by the use of diuretics, with a possible explanation in the fact that lowering global volume of circulating blood may have a significant impact on cerebral circulation. By this analysis, we will be able to take into account the central and cognitive impact of different antihypertensive drugs on the choice of best personalized treatment of hypertension.
S-08-2: RESTORATION OF SIRT3 EXPRESSION IN LIVER ALLEVIATES HIGH-SALT-INDUCED HYPERTENSION
Authors: Gao Peng , You Mei , Li Li , Zhu Zhiming 
Published: January 2023

Objective:
High salt intake is the leading dietary risk factor for hypertension. Although clinical evidence suggests that high salt intake is associated with non-alcoholic fatty liver disease (NAFLD) that is an independent risk factor for hypertension, it remains elusive whether salt-induced hepatic damage leads to the development of hypertension. SIRT3 is the main protector in liver metabolism, and knockout of SIRT3 in mice results in fatty liver and metabolic syndrome. Therefore, we asked whether SIRT3 in liver participated in salt-induced hypertension and investigated the underlying mechanism.

Design and method:
6-week male wild-type or SIRT3-knockout (SIRT3−/−) mice were fed with 0.4% normal salt diet (NSD) or 8% high salt diet (HSD) for 16 weeks. Liver weight, body weight and blood pressure were measured. H&E, Oil-O red, and Sirius Red staining were performed on liver frozen sections to detect steatosis, lipid accumulation and fibrosis, respectively. Hepatic inflammation was reflexed by immunofluorescent staining of Cd11b, Mpo and F4/80, the markers of neutrophils, macrophages and Kupffer cells, respectively. Adeno-associated virus 8 (AAV8) with a Thyroxine-Binding Globulin (TBG) promoter was used to overexpress SIRT3 in liver. Chromatin immunoprecipitation sequencing (ChIP-seq) with an antibody against acetylated histone 3 lysine 27 (H3K27ac), an activated promoter marker, was used to explore the possible mechanism underlying the effect of SIRT3 on salt-induced hepatic inflammation.

Results:
HSD induced a significant increase in systolic blood pressure, a higher liver weight to body weight ratio, and stimulated lipid accumulation, steatosis, and fibrosis in the liver, accompanied by a lowered expression of SIRT3 in liver. Knockout of SIRT3 further exacerbated HSD-induced hepatic steatosis and inflammation, resulting in a more severe hypertension. Restoration of hepatic SIRT3 expression in SIRT3−/− mice by injection of AAV8-TBG-SIRT3 lowered all these pathological changes induced by HSD. ChIP-seq results suggest that knockout of SIRT3 mainly affected the H3K27ac level of promoters of genes related to AMP-activated protein kinase (AMPK) and insulin signaling pathways. Activation of AMPK by metformin significantly blocked salt-induced hepatic inflammation and hypertension, accompanied with an elevated SIRT3 expression in liver.

Conclusions:
The present study proves that the reduced SIRT3 expression in liver plays a critical role in salt-induced hypertension by inducing hepatic inflammation. Restoration of hepatic SIRT3 expression or treatment with metformin helps to stave off hepatic inflammation that underlies salt-induced hypertension in clinical practice.
S-08-4: SELENIUM LEVELS AND FUNCTIONAL STATE OF LIVER IN PATIENTS WITH HYPERTENSION AND NONALCOHOLIC FATTY LIVER DISEASE
Authors: Tverezovska Iryna , Zhelezniakova Natalia 
Published: January 2023

Introduction:
Hypertension (HTN) is significant medical problem due to high levels of morbidity and mortality worldwide. Nonalcoholic fatty liver disease (NAFLD) is chronic disease, with gradual progression and is one of main causes of cirrhosis and hepatocellular carcinoma.
Researches show controversies in Selenium levels in NAFLD patients. According to data, lower Selenium levels can increase risks of development of hypertension, while its increase can promote protective effect. Research in this area is actual due to insufficient data on Selenium metabolism in patients with HTN and NAFLD comorbidity, especially depending on liver damage grade.

Aim:
was to investigate levels of Selenium in patients with hypertension and NAFLD depending on functional state of liver.

Materials and methods:
We examined 100 patients. Main group: 49 hypertensive NAFLD patients (33 (67.3%) females and 16 (32.7%) males). Comparison group: 51 non-hypertensive NAFLD patients (30 (58.8%) females and 21 (41.2%) males).Control group: 20 relatively healthy individuals (11 (55.0%) females and 9 (45.0%) males). Median age in main group was 51.0 [45.0–56.0] years; comparison group: 52.0 [47.0–54.0] years (p = 0.564); control group: 51.0 [45.0–55.5] years.
In the main group hypertension 1 stage was diagnosed in 14 (28.6%) patients, 2 stage: in 35 (71.4%) patients; 1 and 2 grade was diagnosed respectively in 16 (32.7%) and 33 patients (67.3%).
In the main group steatosis was found in 27 (55.1%) patients; steatohepatitis: in 22 (44.9%) patients; in comparison group 30 (58.8%) and 21 (41.2%) patients respectively (p = 0.707).
Selenium levels were assessed using Humal Selenium ELISA kit. Ultrasound of liver was performed according standard procedure using Samsung (Medison) SonoAce X8. Statistical calculations were made using IBM SPSS 25.0.

Results:
Median systolic and diastolic blood pressure in main group was 150.0 [145.0–158.0] mm Hg. and 90.0 [85.0–90.0] mm Hg. In comparison group:125.0 [115.0–130.0] and 80.0 [70.0–80.0] mm Hg. Respectively
Selenium levels were significantly (p < 0.001) increased in patients of comparison group: 67.2 [61.5–77.4] mkg/l vs. 43.5 [39.9–49.1] mkg/l in main group. In control group its levels were 108.0 [96.9–118.8] mkg/l, which was significantly (p < 0.001) higher than in other groups.
Analysis of Selenium levels depending on liver damage grade did not show significant differences. However patients with hypertension and steatohepatitis had higher Selenium levels than patients with steatohepatitis, while non-hypertensive patients had reversed trend (table 1)

Conclusions:
Hypertension is main trigger of decrease in Selenium levels in patients with comorbid NAFLD, which can be connected with its increased metabolism due to providing antihypertensive homeostasis and protection of vascular endothelium via antioxidant mechanisms.
S-09-5: HOMOARGININE AND CARDIOVASCULAR PROTECTION: POTENTIAL THERAPEUTIC TARGET?
Authors: Mels Catharina MC , Mokhaneli Maserame Cleopatra , Böger Rainer , Schwedhelm Edzard , Le Roux Shani Botha 
Published: January 2023

Increasing evidence suggests that homoarginine may offer cardiovascular protection via several mechanisms. We previously investigated prospective associations between homoarginine and i) incident hypertension; ii) carotid wall thickening; and iii) cardiovascular mortality over a five to ten year period in a South African cohort based in the North West Province of the international Prospective Urban and Rural Epidemiology (PURE) study. No significant associations were found between baseline homoarginine levels and incident hypertension (n = 166). However, in a group who remained normotensive over the course of ten years (n = 166), homoarginine levels correlated positively with brachial systolic blood pressure (β = 0.33; p = 0.001); brachial pulse pressure (β = 0.40; p = 0.001); and central pulse pressure (β = 0.30; p = 0.003). This may suggest that the protective effects of homoarginine is not infinite and may become overshowed by other factors once hypertension develops. When investigating prospective associations between homoarginine at baseline and cross-sectional wall area (CSWA) five years later, we indicated that the protective association between CSWA and homoarginine was limited to the female participants of this cohort (n = 396; β = - 0.11; p = 0.024). We also examined whether homoarginine could predict 10-year risk of all cause and cardiovascular mortality.
Higher homoarginine levels were found in survivors when compared to nonsurvivors (n = 143; 1.25 μM vs. 0.89 μM P < 0.001) and in multivariable Cox regression analyses, higher homoarginine predicted a reduction in ten year cardiovascular (standardized hazard ratio, 0.61; 95% CI 0.50 to 0.75) and all cause (standardized hazard ratio, 0.59; 95% CI 0.41 to 0.84) mortality risk. Clearly, it would be beneficial to investigate the regulation of homoarginine levels as therapeutic target in the management of cardiovascular disease. Previous experimental findings indicated that homoarginine infusions have beneficial effects on left ventricular contractility and diastolic function, as well as blood pressure lowering effects to normotensive levels in hypertensive rats. The safety of oral homoarginine levels was established in healthy adults and provided the foundation for two clinical trials to provide data on the optimal dosing of homoarginine in humans (NCT02675660), and to evaluate the applicability of an oral formulation of homoarginine to normalize homoarginine blood levels in patients with acute ischemic stroke (NCT03692234).
S-11-1: ARTERIAL STIFFNESS AND HEMODYNAMIC PARAMETERS FOR THE EARLY PREDICTION OF PREECLAMPSIA: A PROSPECTIVE COHORT STUDY
Authors: Daskalopoulou Stella S. , Phan Kim , Schiller Ian , Dendukuri Nandini , Gomez Yessica Haydee , Gorgui Jessica , Messidi Amira El , Abenhaim Haim , Rahme Elham , Gagnon Robert 
Published: January 2023

Objective:
Preeclampsia is a leading complication of pregnancy, yet there remains no reliable clinical tool for its early prediction. Assessment of vascular dysfunction, a central feature of preeclampsia, by arterial stiffness and hemodynamic measurements could be a promising tool for preeclampsia prediction.
This study aimed to evaluate 1) arterial stiffness and hemodynamic parameters as an early predictive tool for preeclampsia, and 2) longitudinal changes in these parameters and identify changepoints prior to preeclampsia onset.

Design and method:
In this prospective longitudinal cohort study of women with singleton high-risk pregnancies (n = 236), arterial stiffness (carotid-femoral pulse wave velocity, cfPWV) and wave reflection (augmentation index [AIx], and time to wave reflection [T1R]) were assessed using applanation tonometry (SphygmoCor, AtCor) at 10–13 weeks and repeated every 4 weeks throughout pregnancy. Circulating angiogenic biomarkers (soluble fms-like tyrosine kinase, and placental growth factor) were measured (Quantikine, R&D Systems) at each trimester, and a bilateral uterine artery Doppler (UAD) was performed in the second trimester. The predictive ability of arterial stiffness was compared to that of peripheral blood pressure, UAD indices, as well as angiogenic biomarkers.
Furthermore, changepoints in cfPWV, AIx, and T1R were compared between women who did and did not subsequently develop preeclampsia.

Results:
A first-trimester 1 m/s increase in cfPWV was associated with 64% increased odds (p < 0.05), while a 1 ms increase in T1R with 11% decreased odds for preeclampsia (p < 0.01). The area under the curve of arterial stiffness, blood pressure, ultrasound indices, and angiogenic biomarkers was 0.84, 0.68, 0.66, and 0.64, respectively (Figure).
A changepoint in cfPWV was detected at 14–17 weeks. cfPWV then increased in women who subsequently developed preeclampsia but decreased in women who did not; a 1.2 m/s difference in cfPWV between the groups was observed at 22–25 weeks. An increase in AIx was noted at 18–21 weeks while at 30–33 weeks in women who did and did not develop preeclampsia, respectively.

Conclusions:
Arterial stiffness and wave reflection were higher in the first trimester and throughout pregnancy in women destined to develop preeclampsia. These indices predicted preeclampsia earlier (in the first trimester) and with greater ability than blood pressure, UAD, and/or angiogenic biomarkers. Furthermore, altered vascular adaptations in the early second trimester were observed in women who subsequently developed preeclampsia. These findings demonstrate the potential clinical utility of arterial stiffness and hemodynamic parameters as an early screening tool for preeclampsia, which can be used to inform clinical management of high-risk pregnancies.
S-12-1: PAST AND FUTURE OF HYPERTENSION RESEARCH
Authors: Mancia Giuseppe 
Published: January 2023

Research on hypertension has achieved, in the last few decades, important results on the pathophysiology, diagnosis and treatment of the high blood pressure (BP) condition. However, as listed in the 2018 European guidelines, several aspects remain unclarified either because they have not been explored by properly designed studies or because data remain controversial.
This presentation will discuss some of the questions that represent important unmet needs for hypertension research. One, absence of randomized evidence-based trials on the benefit of antihypertensive treatment in important clinical conditions such as white coat hypertension, masked hypertension, resistant hypertension, hypertension of the very elderly people (e.g. nonagenarians) and hypertensives classifiable as frail because of the high number of comorbidities and poor life expectancy. Two, hypertension of relatively young people (e.g. < 45 or 40 years) in whom low event incidence prevents the performance of event-based trials, making prevention or progression of subclinical organ damage probably the only reliable and feasible approach. Three, the benefit of funding treatment on out-of-office (ambulatory and/or home) BP either as an alternative or an addition to classical office BP reduction, a fundamental therapeutic issue never addressed in the past. Four, the BP threshold at which to start treatment in naive hypertensives and the goal BP value to try to reach in different demographic and clinical hypertension subgroups. And finally, the problem of residual risk in apparently well treated hypertensive individuals, i.e. their persistence at a risk level clearly higher than that of the control population. A final consideration will be that assessing this issue by randomized trials will probably be difficult and that future research will thus have to rely more and more frequently on observational studies which can now be based on large population data-bases that extend over many years and can, via extensive information and high quality analysis, substantially reduce the risk of the risk of confounding associated with this approach.