bluuebunny/crossref_metadata_2025_split huggingface dataset:
PS-B02-9: HEART RELATED CIRCULAR RNA (HRCR) INFLUENCES POLYGENIC CARDIAC HYPERTROPHY BY ALTERING CARDIAC MICRORNAS
Authors: Prestes Priscilla Ribeiro , Maier Michelle Carla , Byars Sean , Harrap Stephen B , Charchar Fadi J 
Published: January 2023

Left ventricular hypertrophy (LVH) is the main risk factor for cardiovascular disease after age. Circular RNAs (circRNA), a novel and underexplored RNA type, are created by the back-splicing of pre-mRNA and have been proposed to regulate gene expression post-transcriptionally via targeting micro RNAs (miRNA). Heart related circRNA (Hrcr) has been proposed as an important regulator of LVH and has been shown to be downregulated in mice with cardiac hypertrophy induced by chronic infusion of isoproterenol.
Our aim was to investigate the expression of Hrcr in the hypertrophic heart during development and at maturity. We used the hypertrophic heart rat (HHR), a unique normotensive polygenic model of LVH and heart failure, and its genetic control strain, the normal heart rat (NHR). We first measured the expression of Hrcr by quantitative real-time PCR in left ventricles of HHR and NHR at five ages (2 days old, 4-, 13-, 33- and 50-weeks old). Secondly, we silenced HRCR in human primary cardiomyocytes and performed small RNA sequencing to reveal its miRNA downstream targets. Lastly, we performed in silico investigations to predict miRNA-mRNA targets and explore enriched gene ontology terms in our dataset.We found that Hrcr is upregulated in the HHR at 13-, 33- and 50-weeks old (FC = 2.77-3.57; P < 0.05). The silencing of HRCR revealed novel downstream targets, including four miRNAs (miR-1-3p, miR-330, miR-27a-5p and miR-299-5p) and two noncoding RNAs (ribosomal RNA RNA5-8SP2 and piwi-interacting RNA piR-17153). The miRNA-mRNA interactions predicted a total of 359 mRNAs highly likely to be targeted by the differentially expressed miRNAs. Lastly, 206 gene ontology terms were enriched in our dataset, including terms related to heart development and cardiovascular disease.
PS-C01-10: BLOOD PRESSURE AND OUTCOMES OF TREATED MODERATE HYPERTENSION IN ELDERLY PATIENTS
Authors: Kim Ju Hyeon , Lim Subin , Chung Se Hwa , Yum Yunjin , Joo Hyung Joon , Kim Yong Hyun , Kim Eung Ju 
Published: January 2023

Objective:
There is limited evidence of the growing population of elderly patients with hypertension, particularly the relationship between blood pressure (BP) and adverse outcomes. We sought to estimate outcomes according to attained BP in elderly patients treated for moderate hypertension.

Design and method:
This multicenter, retrospective study used the Korea University Medical Center database built on electronic health records from 2017 to 2020. Patients initiated with at least two antihypertensive drugs as combined therapy were followed up for 3 years. The patients were grouped by on treatment systolic BP (SBP) in 10 mmHg increments from less than 120 to 140 mmHg or more. The primary outcome was a composite of all cause mortality, myocardial infarction, and stroke. The relationships between attained BP and outcomes were assessed using adjusted Cox regression analyses and restricted cubic splines.

Results:
From Jan 2017 to Dec 2017, a total of 4760 patients aged over 75 years (mean age 79.9) were enrolled and grouped into SBP lower 120 mmHg (n = 1188), SBP 120 to 129 mmHg (n = 1221), SBP 130 to 139 mmHg (n = 1194), and SBP over 140 mmHg (n = 1157). After a median follow up of 4.5 years, the incidence of the primary outcome was lowest in individuals with SBP of 120 to 129 mmHg (9.2%, p = 0.087). The age adjusted hazard ratio for the primary outcome showed a J shaped relationship with on treatment SBP and diastolic BP.

Conclusions:
A J curve relationship between on treatment SBP and the composite outcome was found consistently. In real world practice, on treatment SBP less than 130 mmHg was associated with improved outcomes in the elderly with moderate hypertension.
PS-C01-14: IS SHORT STATURE A PROTECTIVE FACTOR IN AN OVERWEIGHT/OBESE AND HYPERTENSIVE OLD AGE POPULATION?
Authors: Paterno Carlos Alberto 
Published: January 2023

Objective:
Age is the most powerful cardiovascular risk factor. Life expectancy is reduced due to hypertension and obesity. There is little information about height and longevity. Geographically in the human population, the short stature was observed as the island effect. The aim of this study is to know whether the short stature is a protective cardiovascular factor in the overweight/obese and hypertensive old age population.

Design & Methods:
For this cross-sectional epidemiological study was registered female-male 1145–729 overweight/obese hypertensive patients with an average age of 54.9–59.2 years. The range of age was from 18 to 94 years. Anthropological data, systolic blood pressure (SBP), diastolic blood pressure (DBP), heart rate (HR), and central hemodynamics parameters (CHP) were compared between the upper and the under quintile of stature, in each decade, and assessed the difference of them. The CHP was measured with a SphygmoCor System PVX, a validated device employing the high-fidelity technique of applanation tonometry according to established protocols. Patients, when entered at the registration were hypertensive with or without treatment or awareness that had hypertension.

Results:
The relation females-males were 1.57, similar to the Italian population 1.51 according to the National Institute of Statistics (census 2021) assessed between the 80 s years to the 90 s years. The body mass index of females-males was 30.4–29.7, respectively. When were assessed the first with the fifth quintile of the stature of each decade, the body mass index, SBP, DBP, HR, central aortic pressure, end-systolic pressure, mean arterial pressure, and pulse pressure does not find a clear statistically significant difference. Obviously, in the upper quintile, the stature and the weight were highest with statistically significant differences. In both sexes with increasing age were observed gradually a population with shorter statures. In this sample the population from the 30 s years to the oldest, the stature decreased in the female group from 162.5 cm to 147.7 cm (difference of 14.8 cm), and in the male group from 176.8 cm to 162.5 cm (difference of 14.3 cm), into both sexes near 2.9 cm by decade.

Conclusion:
High stature, over time, might cause a pernicious overload of work to the cardiovascular system in subjects with several risk factors at old age. The short stature in subjects of both sexes could be a protective factor in a population of older overweight/obese hypertensive, a fact more evident in the female group than the male group.
PS-C03-11: FACTORS ASSOCIATED WITH BLOOD PRESSURE CHANGES IN RESPONSE TO DIGITAL THERAPEUTICS SYSTEM: A RETROSPECTIVE ANALYSIS OF THE HERB-DH1 TRIAL
Authors: Aga Mitsuharu , Hisaki Fumi , Tanigawa Tomoyuki 
Published: January 2023

Digital therapeutics are evidence-based therapeutic interventions driven by software. Recently, the HERB Digital Hypertension 1 (HERB-DH1) pivotal trial was conducted to investigate the efficacy of the digital therapeutics in patients with hypertension. It has demonstrated that the difference between digital therapeutics group and control group in mean change in 24 h ambulatory SBP from baseline to 12 weeks was -2.4 (95% confidence interval [CI]: -4.5 to -0.3). However, The mechanism that led to the decrease in blood pressure has not been elucidated.
We retrospectively analyzed the digital therapeutics group (HERB system + standard lifestyle modification) of the HERB-DH1 trial. Spearman's correlation test between the blood pressure change from baseline to 12 weeks and clinical variables in patients with hypertension was performed. Of the 199 patients, 148 patients with available data for initial and final blood pressures were included. In the univariate analysis, age (r = -0.1761; 95% CI: -0.3324 to -0.01033; P = 0.0323), baseline blood pressure (r = -0.3697; 95% CI: -0.5048 to -0.2170; P < 0.0001), body weight change (r = 0.1704; 95% CI: 0.004451 to 0.3271; P = 0.0385), baseline BMI (r = -0.1738; 95% CI: -0.3303 to -0.008007; P = 0.0346) and BMI change (r = 0.1653; 95% CI: -0.0007627 to 0.3225; P = 0.0447) were related to blood pressure change. In conclusion, it is indicated that intervention in the digital therapeutics group of HERB-DH1 trial leads to lifestyle modification and body weight change, which impact on effective blood pressure control.
PS-C04-6: ASSOCIATION BETWEEN SERUM URATE LEVEL AND CAROTID ATHEROSCLEROSIS: AN INSIGHT FROM A POST-HOC ANALYSIS OF THE RANDOMIZED CLINICAL TRIAL (PRIZE)
Authors: Tanaka Atsushi , Node Koichi 
Published: January 2023

Objectives:
Elevated serum urate (SU) levels are associated with arterial atherosclerosis and subsequent cardiovascular events. However, an optimal therapeutic target SU level for delaying atherosclerotic progression in patients with hyperuricemia remains uncertain. The aim of this analysis was to assess an association between changes in SU level and carotid intima-media thickness (IMT) to examine whether an optimal SU concentration exists to delay atherosclerotic progression.

Design and method:
This was a post-hoc analysis of the PRIZE (program of vascular evaluation under uric acid control by xanthine oxidase inhibitor, febuxostat: multicenter, randomized controlled) study of Japanese adults with asymptomatic hyperuricemia (UMIN000041322). Among the full analysis set population treated with febuxostat (n = 239), subjects who had both data on SU level and mean common carotid artery (CCA)-IMT at baseline and 24 months were included in the present analysis. The primary endpoint of this analysis was an association between changes in SU levels and mean CCA-IMT after 24 months of febuxostat treatment.

Results:
A total of 204 eligible subjects were included in this analysis. The mean age was 69.1 +/- 9.6 years, and one fifth were female. Most had a history of treated hypertension and/or dyslipidemia. The mean baseline SU level was 7.7 ± 1.0 mg/dL, and febuxostat treatment significantly reduced SU concentrations at 24 months (estimated mean change -3.1 mg/dL, 95% confidence interval -3.2 to -2.9). A multivariable linear regression analysis revealed that a reduction in SU level was associated with changes in mean CCA-IMT values at 24 months (Figure A). In contrast, the achieved SU concentrations were not associated with changes in mean CCA-IMT at 24 months (Figure B).

Conclusion:
A greater reduction in SU, but not its achieved concentrations, may be associated with delayed progression of carotid IMT in patients with asymptomatic hyperuricemia treated with febuxostat.
PS-C04-10: THE ASSOCIATION OF ORAL HYPOFUNCTION AND ARTERIAL STIFFNESS
Authors: Matsumoto Chisa , Tomiyama Hirofumi , Matsuura Mari , Shiina Kazuki , Nakano Hiroki , Yamashina Akira 
Published: January 2023

Objective:
Frailty is associated with higher risk of cardiovascular disease (CVD) and mortality. Recently, oral hypofunction, a disease in which the oral function is complexly reduced not only by ageing but also by a variety of factors, is regarded as a major risk factor for frailty, as it develops malnutrition and sarcopenia. However, no studies have evaluated the association of oral hypofunction and arterial stiffness, a marker for CVD. The objective of this study is to evaluate the association of oral hypofunction and arterial stiffness.

Design and method:
Japanese subjects above 50 years old who underwent annual health checkup were enrolled in this cross-sectional study. Subjects with history of CVD and dementia were excluded. Arterial stiffness was evaluated by brachial-ancle pulse wave velocity (baPWV). Oral hypofunction was evaluated based on the guidance by the Japanese Association for Dental Science. Seven oral functional factors, oral hygiene, oral moisture, occlusal force, tongue-lip motor function, tongue pressure, masticatory function, and swallowing function were assessed. Oral hypofunction was diagnosed if more than 3 factors showed deterioration. The association of oral hypofunction and baPWV was evaluated by multivariate linear regression analysis adjusted for conventional CVD risk factors. We also performed secondary analysis to evaluate the association of each oral functional factors and arterial stiffness.

Results:
Among 138 subjects (mean age: 59 ± 6 years), 29 subjects (21%) had oral hypofunction. After adjustment for CVD risk factors, oral hypofunction was not significantly associated with baPWV (β; = 24 ± 52, p = 0.65). In secondary analysis, higher oral bacterial count, a marker of oral hygiene, was significantly associated with increased baPWV even after adjustment for conventional CVD risk factors (1SD increase of oral bacterial count; β; = 51 ± 23, P = 0.03). But, this association was not observed with other oral functional factors and arterial stiffness.

Conclusions:
Oral hypofunction was not associated with increase of arterial stiffness. However, higher oral bacterial count was associated with increased arterial stiffness. Further research on oral hypofunction and CVD is warranted.
PS-C04-11: EFFECT OF ANKLE BRACHIAL INDEX EXAMINATION ON CENTRAL AORTIC BLOOD PRESSURE IN PATIENTS WITH PERIPHERAL ARTERY DISEASE
Authors: Sonoda Shinjo , Miura Toshiya , Anai Reo , Kashiyama Kuninobu , Natsuaki Masahiro , Node Koichi 
Published: January 2023

Background:
Ankle brachial index (ABI) measurements are useful for detecting peripheral artery disease (PAD) as a screening test. In general, ABI examinations are contraindicated in patients with aortic aneurysm and history of aortic aneurysm. However, little is known whether ABI examinations elevate central aortic blood pressure (CBP).

Method:
From June 2018 to August 2020, we prospectively analyzed 66 patients who were performed cardiac catheterization through femoral approach and divided into peripheral artery disease complicated (PAD, N = 27) and uncomplicated (non-PAD, N = 39) groups. After insertion of pigtail catheter into both ascending (Asc), abdominal (Abd) positions, resting CBPs at Asc and Abd were measured. Subsequently, we measured each CBP during ABI, respectively. We compared resting CBP (R-CBP) and CBP during ABI (ABI-CBP) between PAD and non-PAD groups.

Results:
In non-PAD group, ABI-CBP at Abd was significantly higher than BL-CBP (P < 0.05). On the other hand, there were no significant ABI-CBP elevation at Asc and Abd in PAD. There was no case with CBP elevation more than 20mmHg during ABI measurements in both groups. Furthermore, no clinical events were observed after ABI measurements in both groups.

Conclusion:
ABI was considered a safe testing in patients with suspected PAD.
PS-C05-3: ESTIMATION OF PULMONARY PULSATILE ARTERIAL LOAD IN PATIENTS UNDERGOING INVESTIGATION FOR PULMONARY HYPERTENSION
Authors: Adji Audrey , Kearney Katherine , Hungerford Sara , Kotlyar Eugene , Lau Edmund , Jabbour Andrew , Hayward Christopher 
Published: January 2023

Objective:
Diagnosis of pulmonary hypertension (PH) is typically confirmed by elevation of the pulmonary artery (PA) pressure and/or pulmonary vascular resistance (PVR). However, PVR may overlook the pulsatile component of RV hydraulic work which involves the dynamic interplay between PA resistance and compliance. PA vascular impedance (PVZ) offers a more comprehensive assessment of RV and PA load. This study aims to apply a method of PA pulsatile load as PVZ determination in a cohort of patients referred for PH evaluation.

Design and Methods:
Fifty-eight participants (71% females, mean 53 ± 18 years) were recruited, and underwent right heart catheterisation (RHC) to measure systolic (sPAP), diastolic (dPAP), mean (mPAP), and PA wedge pressures (PAWP), cardiac output (CO) and PVR; classified as precapillary PH (PrecPH), isolated postcapillary PH (IpcPH), combined PH (CpcPH) and control group. PA flows were recorded using 3T cardiac magnetic resonance (CMR); RV volume (V) were obtained by contouring endocardial and epicardial borders on the short axis at end diastolic (ED) and end systolic (ES) phases, and ejection fraction (RVEF) was calculated as (RVEDV - RVESV)/RVEDVx100. Volumetric flow was measured using phase-contrast velocity quantification using 3T cardiac magnetic resonance (CMR). PVZ was expressed as the relationship of pulsatile pressure to pulsatile flow in the frequency domain.

Results:
76% of patients had evidence of PH on RHC (8 PrecPH, 17 IpcPH, 9 CpcPH). CpcPH and PrecPH patients tended to show poorer RV contractility compared with control and IpcPH groups, albeit not statistically significant. PVZ was highest in patients with PrecPH and CpcPH, while lowest in those with IpcPH. A correlation analysis was performed to seek association between mPAP, PVR and PVZ and other variables thought to be related to PH. In PrecPH, increased mPAP was found to be associated with raised PVR (p < 0.001; R = 0.94) and PVZ (p < 0.001; R = 0.78). However, there was no association between elevated mPAP and PVZ in IpcPH and CpcPH, despite a significant correlation with PVR (IpcPH P = 0.03, R = 0.55; CpcPH P = 0.01, R = 0.77).

Conclusion:
We describe a straightforward method to measure and compare pulsatile vascular load of the systemic and pulmonary circulations simultaneously, allowing further quantification of systemic/pulmonary vascular interactions. Further research is required to evaluate small-vessel contributions to the arterial circulation, especially in the pulmonary system.
PS-C05-5: CLINICAL IMPACT OF CREDO-KYOTO RISK SCORE ON IN-HOSPITAL BLEEDING IN PATIENTS WITH ACUTE CORONARY SYNDROME
Authors: Natsuaki Masahiro , Minematsu Yuki , Yoshioka Goro , Shinzato Kodai , Node Koichi 
Published: January 2023

Background:
CREDO-Kyoto bleeding risk score was developed to predict the post-discharge bleeding events in patients with percutaneous coronary intervention. However, there were limited reports of the effectiveness of this score to predict the in-hospital bleeding events in patients with acute coronary syndrome (ACS).

Methods:
We evaluated 562 consecutive ACS patients in Saga university hospital between 2014 and 2019. Primary outcome was major bleeding during hospitalization. Major bleeding was defined as the GUSTO moderate/severe bleeding. Patients were classified into three groups according to the CREDO-Kyoto bleeding risk score (low, intermediate and high).

Results:
Major bleeding events occurred in 12.1% of all patients during hospitalization. Patients in the high risk group (n = 22) had significantly higher incidence of major bleeding than those in the intermediate (n = 113) and the low risk groups (n = 427) (22.7%, 18.6%, versus 9.8%, respectively, p = 0.02). Multivariate analysis showed that intermediate and high risk groups were independent predictors for the in-hospital major bleeding.

Conclusions:
CREDO-Kyoto risk score successfully identified high risk ACS patients for the major bleeding during hospitalization.
PS-C05-12: OMENTIN-1 PREDICTS MEMBRANE FLUIDITY OF RED BLOOD CELLS AND MICROCIRCULATORY FUNCTION IN HYPERTENSIVE SUBJECTS BY THE NITRIC OXIDE-DEPENDENT MECHANISM
Authors: Tsuda Kazushi 
Published: January 2023

Objective:
It has been shown that abnormalities in physical properties of the cell membranes may strongly be linked to hypertension. Recent evidence indicates that omentin-1, an adipokine with anti-inflammatory properties, may actively participate in the pathophysiology of hypertension, atherosclerosis and other cardiovascular diseases. However, few studies have evaluated the association between omentin-1 and membrane function in hypertension. The present study was performed to investigate possible relationships among omentin-1, endothelial function and membrane fluidity (a reciprocal value of membrane microviscosity) of red blood cells (RBCs) in patients with essential hypertension and normotensive subjects.

Design and method:
We measured the membrane fluidity of RBCs with an electron spin resonance (ESR) and spin labeling method. In each ESR spectrum, we calculated the order parameter (S) for the spin-label agent (5-nitroxide stearate). The greater the value of the order parameter (S) was, the lower the membrane fluidity of RBC was.

Results:
(1) The order parameter (S) obtained from the ESR spectra of RBC membranes was significantly greater in hypertensive subjects (0.727 ± 0.001, n = 64) than in normotensive subjects (0.719 ± 0.001, n = 49, P < 0.0001), indicating that membrane fluidity of RBCs might be reduced in hypertensive subjects compared with normotensive subjects. (2) Plasma omentin-1 levels were lower in hypertensive subjects than in normotensive subjects (HT 0.486 ± 0.014 ng/mL, n = 64, NT 0.537 ± 0.019 ng/mL, n = 49, P < 0.05). Plamsa omentin-1 significantly correlated with plasma nitric oxide (NO)-metabolite levels (an index of endothelial function) (r = 0.392, n = 113, P < 0.0001). (3) Furthermore, the order parameter (S) of RBCs inversely correlated with both plasma omentin-1 (r = -0.347, n = 113, P < 0.001) and plasma NO-metabolites, suggesting that reduced membrane fluidity of RBCs might be associated with decreased levels of plasma omentin-1 and endothelial dysfunction. (4) Multivariate regression analysis showed that, after adjusting for confounding factors, plasma omentin-1 might be an independent predictor of membrane fluidity of RBCs.

Conclusions:
The ESR study demonstrated that lower plasma omentin-1 levels might have a close correlation with reduced membrane fluidity of RBCs and impaired hemorheologic behavior in hypertensive subjects by the NO-dependent mechanism. It is strongly suggested that plasma omentin-1 could be a putative biomarker for the microcirculatory function in hypertension.
PS-C06-2: EXTRACELLULAR VESICLES AS DIAGNOSTIC BIOMARKER OF ARTERIAL HYPERTENSION A COMPARATIVE OBSERVATIONAL STUDY
Authors: Raghav Alok , Giri Richa , Agarwal Saurabh , Singh Manish 
Published: January 2023

Objective:
Arterial hypertension is a major cardiovascular risk factor that contributes to cardiovascular diseases and associated target organ injuries. World Health Organization estimated that hypertension affected around 1.28 billion adults aged between 30 to 79 years worldwide making it a prime cause of premature death.

Design:
Observational study

Methods:
The present study compared the blood derived extracellular vesicles from the American Heart Association guidelines classified study subjects as normal elevated stage 1 stage 2 and hypertensive crisis. EVs were isolated and characterized for surface tetraspanin expression marker CD 41 using flow cytometer and nano tracking analysis Total protein from each sample was extracted and mass spectrometry was performed.

Results:
Study subjects with mean age of 57 years with arterial hypertension more prevalent in women 62 percent compared to men 38 percent. Positive expressions of tetraspanin CD41 were found in EVs isolated from all patients of each group. Nanotracking analysis of isolated EVs showed an average size of 167 nm with no comparable differences among study groups. Significant differences were found in the mass spectrometric analysis within the study groups in 28 proteins compared using ANOVA with the Kruskall Wallis test followed by Dunns test.

Conclusion:
The results of the study concluded that proteomic analysis of isolated proteins within the study groups can be explored as diagnostic biomarkers for screening of arterial hypertension and these specific proteins can be further explored for fabricating point of care devices for a community screening of arterial hypertension