bluuebunny/crossref_metadata_2025_split huggingface dataset:
Diffuse alveolar haemorrhage as the initial presentation of systemic lupus erythematosus possibly triggered by Rhogam injection in a 24-year-old pregnant woman: A case report
Authors: Khanmirzaei Amir , Bozorgi Maryam , Azarinoush Gelareh , Aghaei Fatemeh , Bigdeli Asiye , Jazi Kimia , Asghari Akram , Masoumi Maryam 
Published: January 2025

ABSTRACT
Diffuse alveolar haemorrhage (DAH) is an uncommon and potentially life-threatening occurrence in systemic lupus erythematosus, involving bleeding into the alveolar space caused by the disruption of the alveolar capillary basement membrane. We present a 24-year-old Persian woman with a complaint of progressively worsening shortness of breath following the administration of intramuscular Rhogam after 3 days. According to her worsening clinical condition, the pregnancy was terminated. She was admitted to the intensive care unit and intubated. Simultaneously, she developed fungal and bacterial pneumonia tolerant to therapies. After several investigations, the patient was finally diagnosed with systemic lupus erythematosus along with DAH, as the first presentation of the disease. This is the first case of a pregnant woman experiencing DAH as a lupus flare following Rhogam injection. Clinicians should be aware of the mimicry nature of lupus and the importance of immune reactions in these patients.
Tax-price elasticities of charitable giving and selection of reporting: panel study of South Korea
Authors: Kato Hiroki , Goto Tsuyoshi , Kim Youngrok 
Published: January 2025

Abstract
In this study, we estimate the tax-price elasticity of charitable giving and address the bias caused by the existence of unreported donations and self-selection regarding reporting on giving. To eliminate this bias, we propose a simple estimation method based on intention-to-treat analysis. Using our proposed method and the exogenous variation in tax incentives in the 2014 South Korean tax reform, we estimate the price elasticity to be −3.5 for the amount of total giving, which is more elastic than that in the standard results that do not account for unreported donations and self-selection. This result implies that the 2014 tax reform reduced the total amount of giving and that tax incentives should be expanded.
Subgenome-informed statistical modeling of transcriptomes in 25 common wheat accessions reveals cis- and trans-regulation architectures
Authors: Nomura Yasuyuki , Okada Moeko , Tameshige Toshiaki , Takenaka Shotaro , Shimizu Kentaro K , Nasuda Shuhei , Nagano Atsushi J 
Published: January 2025

Abstract
Common wheat is an allohexaploid, where it is difficult to obtain homoeolog-distinguished transcriptome data. Lasy-Seq, a type of 3ʹ RNA-seq, is technology efficient at obtaining homoeolog-distinguished transcriptomes. Here, we applied Lasy-Seq to obtain transcriptome data from the seedlings, second leaves, and root tips of 25 common wheat lines mainly from East Asia. Roots and seedlings were similar to each other in transcriptome profiles, but they were different from the leaves. We then asked how three homoeologous genes from different subgenomes (i.e. triads) show different levels of expression. Specifically, we examined the effects of subgenomes, lines, and their interaction on the expression levels of each homoeolog triad, separately in each tissue. Of the 19 805 homoeolog triads, 51–55% showed significant effect of subgenome, suggesting cis-regulation, whereas 24–30% showed significant effect line, suggesting trans-regulation. We also found that 7.7–9.0% triads showed significant effects of the interaction. Hierarchical clustering and co-trans regulation network analysis of homoeolog triads revealed that the patterns of expression polymorphisms among the lines were shared in different genes. Our results also implied that expression variation between lines is caused by changes in a smaller number of common trans-factors. We performed gene ontology (GO)-term enrichment analysis using newly annotated and substantially improved GO annotations, which revealed that GO terms related to each tissue-type function were enriched in genes expressed in the leaves and roots. Our information provides fundamental knowledge for the future breeding of plants possessing complex gene regulatory networks such as common wheat.
Lost in “Translation-Work”: Negotiations of Lost and Saved Language Among Ex-Charismatic Christians in Norway
Authors: Gilsvik Espen 
Published: January 2025

Abstract
This article explores the language negotiations among a group of Norwegian Christian Charismatic disaffiliates from a Facebook community called “The Journey” (no. “Reisen”). The analysis is based on 24 in-depth interviews and examines specific language practices of the disaffiliates, which I call translation-work. Translation-work involves substituting the religious meaning of Christian Charismatic concepts with secular meaning, allowing the term to remain in the disaffiliates’ vocabulary. Such language negotiations also reframe Charismatic experiences with secular words and actively omit relevant disaffiliation terms and concepts used in American discourse on disaffiliation. The Norwegian informants are particularly skeptical of victim-oriented terms even though they adequately fit such descriptions. The article discusses these omissions and how and why the informants engage in translation-work. It further suggests that established scholarly and popular disaffiliation terms do not precisely capture the nuances of non/religious language in this material. The article contributes to the field by providing thick descriptions of the informants’ negotiations and suggesting translation-work as a conceptualization, which gives us new sociological understandings of liminal non/religious language and experiences.
Evaluation of suitability of two tools for the diagnosis of canine visceral leishmaniasis
Authors: Garcia Valeria S , Gutiérrez Maira S , Gonzalez Verónica D G , Gugliotta Luis M , Burna Alexis , Eiras Diego , Cabeza Matías S , Arias Diego G , Guerrero Sergio A 
Published: January 2025

Abstract

Background
Leishmaniasis still imposes a heavy burden on many health systems and remains a public health problem. Early diagnosis in dogs, which act as a major reservoir for the pathogen, is central. The intention of epidemiologic studies is to identify the disease early, allowing rapid intervention to reduce its effects. This study aimed to develop two types of diagnostics tools, an enzyme-linked immunosorbent assay (ELISA) and a lateral flow immunochromatography assay for recognizing canine visceral leishmaniasis (CVL).

Methods
Multiwell ELISA plates were sensitized and strips were designed with the same chimeric recombinant antigen from Leishmania spp. (LeQuiDi) and the results were compared. The sera panel contained 409 samples, including 244 positives and 165 negatives. Of the 165 negative sera, 67 were obtained from Corrientes city, an endemic area for CVL in Argentina, and 98 of this group from La Plata city, a non-endemic area in Argentina (they were seropositive for other infections, as specificity control).

Results
Serum with an elevated concentration of lipids, or bilirubin, or a variable percentage of haemolysis were tested without interference. Both the sensitivity and specificity of the LeQuiDi-based tests were high: ≥93% for ELISA and ≥87% for LFIA.

Conclusions
Our study introduces LeQuiDi as a promising antigen for CVL diagnosis, regardless of the test format.
MYBL2 promotes proliferation of clear cell renal cell carcinoma by regulating TOP2A and activating AKT/mTOR signaling pathway
Authors: Huang Yawei , Xi Xiaoqing , Ye Zhenfeng , Zhang Chiyu , Jiang Yi , Yu Fanfan , Huang Gaomin 
Published: January 2025

AbstractRenal cell carcinoma (RCC) is one of the most common malignancies in the urinary system, and clear cell renal cell carcinoma (ccRCC) is the most common subtype. MYBL2 has been reported to be overexpressed in various tumors and associated with poor prognosis in patients, but its biological role in ccRCC remains unclear. In this study, we investigated the mRNA and protein expression levels of MYBL2 in ccRCC samples and evaluated the prognostic value of MYBL2 using TCGA dataset. In vitro functional assays were performed using CCK‐8, EdU, colony formation, cell scratch, and transwell assays, as well as in vivo tumorigenesis assays to investigate the biological functions of MYBL2 in ccRCC. Additionally, gene set enrichment analysis (GSEA) was used to explore the downstream pathways of MYBL2, which were further validated. Finally, we predicted the target genes of MYBL2 using bioinformatics and validated them using ChIP and dual‐luciferase reporter gene assays. MYBL2 expression was significantly higher in ccRCC than in adjacent normal tissues and was associated with poor prognosis. MYBL2 expression was positively correlated with the pathological tumor grade and clinical TNM stage of ccRCC patients. Knockdown of MYBL2 significantly inhibited the proliferation of renal cancer cells in vitro and in vivo, and knockdown of MYBL2 could inhibit cell invasion and migration, while overexpression of MYBL2 had the opposite effect. GSEA revealed that MYBL2 was associated with the mTOR signaling pathway and cell cycle pathway, which was confirmed by our study. Finally, we found that TOP2A was a target gene of MYBL2, and MYBL2 could bind to the TOP2A promoter to regulate its transcriptional activity, promoting the proliferation of clear cell renal cell carcinoma cells. MYBL2 emerges as a highly expressed factor that significantly correlates with adverse patient prognosis in ccRCC. Mechanistically, MYBL2 transcriptionally upregulates TOP2A, thereby modulating the proliferation of ccRCC cells. Furthermore, MYBL2 activates the mTOR signaling pathway, a critical node in the progression of ccRCC. Collectively, these findings position MYBL2 as a promising candidate for both a biological marker and a therapeutic target in the management of ccRCC.
Targeting the insular cortex for neuropathic pain modulation: Insights into synaptic and neuronal mechanisms
Authors: Kim Kyeongmin , Nan Guanghai , Kim Hee Young , Cha Myeounghoon , Lee Bae Hwan 
Published: January 2025

AbstractNeuropathic pain, caused by nerve damage, greatly affects quality of life. Recent research proposes modulating brain activity, particularly through electrical stimulation of the insular cortex (IC), as a treatment option. This study aimed to understand how IC stimulation (ICS) affects pain modulation. In a rat neuropathy model, researchers used optogenetic and ICS techniques to evaluate changes in mechanical allodynia and synaptic changes, focusing on glutamate receptors (AMPAR, NR2A, NR2B). Optogenetic inhibition of IC neurons relieved pain without altering synaptic plasticity. However, repetitive ICS combined with optogenetic activation diminished the pain‐relieving effects of ICS and increased AMPAR and NR2B receptor levels. Additionally, activating inhibitory neurons also reduced pain, while repetitive activation of excitatory neurons lessened the effectiveness of ICS and was associated with heightened receptor expression. These findings suggest that inhibiting excitatory neurons or activating inhibitory neurons in the IC could help modulate pain in neuropathic conditions, shedding light on how ICS can influence pain management through changes in synaptic plasticity.
Analysis of the Biomechanical Effects of Vertebral Body Tethering With Apical Fusion
Authors: Frank Jil , Trobisch Per David , Pishnamaz Miguel , Hildebrand Frank , Praster Maximilian 
Published: January 2025

Study Design.
Biomechanical study by using a multibody simulation approach.

Objective.
Objectification of spinal biomechanics after vertebral body tethering with and without apical fusion.

Summary of Background Data.
Vertebral body tethering, a motion-preserving surgical technique for correction of adolescent idiopathic scoliosis, is increasingly being used for thoracolumbar curves. However, tether breakage remains a common problem with breakage rates up to 60% for TL curves. Therefore, surgeons have begun to adapt their surgical technique by fusing the apex. The short-term clinical studies show a significant reduction of the tether breakage rate to 10%, but little is known about the biomechanical reasons. Therefore, this study analyzes the intervertebral compression and tether force in a tethered spine without apical fusion and in a tethered spine with apical fusion between L1/2.

Methods.
A multibody simulation approach was chosen to analyze the biomechanical effects of two surgical techniques during different physiological movements. The tether and intervertebral compression forces in the different instrumented segments are once analyzed for a T10 to L3 tethered spine and once for a T10 to L3 tethered with additional L1/2 fusion using an intervertebral cage.

Results.
VBT with apical fusion reduces the prevailing tether forces not only at the fused level by nearly 861 N but also at the adjacent spinal segments by around 100 N. However, a significant increase in intervertebral compression force of ~706 N can be observed, especially at the adjacent spinal segments.

Conclusion.
L1/2 fusion in a tethered spine reduces tether forces in adjacent segments and thus might decrease the rate of tether breakage. However, fusion results in increased intervertebral compression forces by up to 31% compared with an unfused spine. Long-term clinical studies are needed to further analyze and evaluate the biomechanical consequences.
Wide pulse pressure as a novel predictor of contrast-induced acute kidney injury in diabetic patients undergoing primary percutaneous coronary intervention
Authors: Yilmaz Cemalettin , Güvendi Şengör Büşra , Zehir Regayip , Kaya Ahmet Ferhat , Özdil Mehmet Hasan , Kültürsay Barkin 
Published: January 2025

Background
Primary percutaneous coronary intervention (p-PCI) is pivotal in managing ST-segment elevation myocardial infarction (STEMI); however, it introduces potential risks, such as contrast-induced acute kidney injury (CI-AKI). This study aimed to investigate the relationship between pulse pressure (PP) and CI-AKI incidence in diabetic patients.

Methods
In this retrospective study conducted between 2021 and 2022, 590 diabetic STEMI patients undergoing p-PCI were categorized based on the presence of wide PP. Individuals with PP ≥ 65 mmHg were classified as the ‘wide PP (+) group’, while those with PP < 65 mmHg were classified as the ‘wide PP (−) group’. To determine independent predictors of CI-AKI, multivariable logistic regression models were applied. After establishing the base model, blood pressure indices, including PP, SBP, DBP, mean arterial pressure, and pulsatility were added to the model using the stepwise selection method.

Results
Among the patients, 18.3% (n = 108) were in the wide PP (+) group, while 81.7% (n = 482) were in the wide PP (−) group. The incidence of hypertension, SBP, PP, and the risk of CI-AKI were higher in the wide PP (+) group. Multivariable analysis recognized PP, wide PP, and pulsatility as independent CI-AKI predictors [odds ratio (OR): 1.024, 95% confidence interval (CI): 1.003–1.045, P = 0.025; OR: 1.684, 95% CI: 1.025–2.769, P = 0.040; OR: 13.816, 95% CI: 2.069–92.245, P = 0.007, respectively].

Conclusion
Increased PP emerges as an independent predictor for CI-AKI in diabetic patients undergoing p-PCI.
Seizure prediction and forecasting: a scoping review
Authors: Cheng Joshua C. , Goldenholz Daniel M. 
Published: January 2025

Purpose of review
This scoping review summarizes key developments in the field of seizure forecasting.

Recent findings
Developments have been made along several modalities of seizure forecasting, including long term intracranial and subcutaneous encephalogram, wearable physiologic monitoring, and seizure diaries. However, clinical translation of these tools is limited by various factors. One is the lack of validation of these tools on an external dataset. Moreover, the widespread practice of comparing models to a chance forecaster may be inadequate. Instead, the model should be able to at least surpass a moving average forecaster, which serves as a ‘napkin test’ (i.e., can be computed on the back of a napkin). The impact of seizure frequency on model performance should also be accounted for when comparing performance across studies. Surprisingly, despite the potential for poor quality forecasts, some individuals with epilepsy still want access to imprecise forecasts and some even alter their behavior based upon them.

Summary
Promising advances have been made in the development of tools for seizure forecasting, but current tools have not yet overcome clinical translation hurdles. Future studies will need to address potentially dangerous patient behaviors as well as account for external validation, the napkin test, seizure frequency dependent metrics.
Multi-Institutional Analysis of Pancreatoduodenectomy for Nonfamilial Periampullary Adenoma: A Novel Risk Score to Guide Shared Decision-Making
Authors: Mavani Parit T , Sok Caitlin , Eng Nina , Marra Angelo , Foroutani Laleh , Alseidi Adnan , Hariri Hussein , Wilson Gregory , Ahmad Syed A , Scoggins Charles , Hester Caitlin , Datta Jashodeep , Merchant Nipun , LeCompte Michael , Kim Hong Jin , Sigler Gregory , Zafar Nabeel , Weber Sharon , Prela Orjola , Carpizo Darren , Kasting Christina , Fields Ryan , Sarmiento Juan M , Russell Maria C , Shah Mihir M , Maithel Shishir K , Kooby David A 
Published: January 2025

Introduction:
Pancreatoduodenectomy (PD) may occasionally be indicated for complete removal of periampullary (duodenal and ampullary) adenomas (PAs). As compared with malignant indications, PD for benign or pre-malignant disease is often associated with increased morbidity. While the Spigelman classification assesses malignancy risk for familial adenomatous polyposis (FAP)-related duodenal adenomas, no malignancy risk score (MRS) exists for non-FAP related PAs. We developed a MRS for non-FAP related PAs undergoing PD to weigh risk of malignancy and postoperative morbidity.

Methods:
We retrospectively analyzed patients with non-FAP related PA who underwent PD at eight institutions (2010-2022). Patient and lesion factors associated with final malignant pathology were identified using multivariable logistic regression to create MRS. Postoperative complications were assessed according to MRS.

Results:
Of 127 patients, 59 (46.5%) had evidence of malignancy on final pathology. The odds of malignancy were higher in patients with older age ≥65 years (OR3.2, p=0.01), bile duct ≥9mm (OR3.3, p=0.009), preoperative symptoms (OR7.7, p=0.002), and high-grade dysplasia (HGD, OR7.5, p<0.001). A MRS was derived ranging from 0 to 6: age≥65=1, bile duct≥9mm=1, symptomatic=2, HGD=2. Patients were stratified into low (MRS1–2, n=26), intermediate (MRS3–4, n=59), and high-risk groups (MRS5–6, n=26), with malignancy rates increasing with MRS (10.3%, 44.1%, and 88.2%, p<0.001). Patients in the no/low-risk group (MRS0-2) had higher odds of major postoperative complications compared with patients in the intermediate/high-risk group (MRS≥3, OR2.9, p=0.047).

Conclusion:
This novel MRS stratifies the risk of malignancy in non-FAP related PAs managed with PD. This score can be used to counsel patients who may require PD for complete tumor removal about their risk of harboring malignancy and their risk of major postoperative complications.
Single-cell digital twins identify drug targets and repurposable medicine in Alzheimer’s disease
Authors: Ren Yunxiao , Hu Ming , Li Yang E. , Pieper Andrew A. , Cummings Jeffrey , Cheng Feixiong 
Published: January 2025

AbstractAlzheimer’s disease (AD) is a complex and poorly understood neurodegenerative disorder without sufficiently effective treatments. Novel approaches to identify FDA-approved drugs that may hold potential for mitigating symptoms of AD hold promise for addressing this problem. One such strategy is the use of digital twins (DTs), which are virtual representations of physical entities that facilitate therapeutic target identification by accurately characterizing disease heterogeneities in real time through continuous feedback and dynamic model updates. In this study, we developed a single-cell digital twin (scDT) framework using single-nuclei RNA-seq (1,197,032 nuclei) and ATAC-seq (740,875 nuclei) data from the middle temporal gyrus of 84 donors across 4 degrees of AD neuropathological change (ADNC). We observed differential gene expression for six major cell types intensified at severe ADNC. We also constructed cell type-specific transcription factor (TF)-target gene networks by leveraging peak-to-gene linkages and motif enrichment analyses. By integrating genome-wide association study (GWAS) loci with cell type-specificcis-regulatory DNA elements (CREs), we identified 141 ADNC-associated genes. Using gene set enrichment analysis (GSEA) and network proximity analysis, we identified 13 candidate repurposable drugs that were associated with these ADNC-associated genes. In summary, we constructed a single-cell digital twin (scDT) framework cell type-specific target identification and drug repurposing in AD and other complex diseases if broadly applied.
TUB-010, a novel anti-CD30 antibody-drug conjugate based on Tub-tag technology, widens the therapeutic window by reducing toxicity while maintaining high efficacy
Authors: Gerlach Marcus , Schmitt Saskia , Cyprys Philipp , Kasper Marc-André , Mai Isabelle , Klanova Magdalena , Maiser Andreas , Leonhardt Heinrich , Hackenberger Christian P. R. , Fingerle-Rowson Guenter R. , Vogl Annette M. , Schumacher Dominik , Helma Jonas 
Published: January 2025

AbstractTUB-010 is a next-generation antibody-drug conjugate (ADC) targeting CD30, which is expressed on various hematopoietic malignancies such as Hodgkin lymphoma and anaplastic large cell lymphoma. Patients with refractory and relapsed CD30-positive cancers often lack effective and tolerable therapy options. Among the therapeutic options for these patients is brentuximab vedotin (Adcetris), a monomethyl auristatin E (MMAE)-delivering anti-CD30 ADC with a mean drug-to antibody ratio (DAR) of 4. Adcetris exhibits a high response rate at the cost of significant toxicities, among which neutropenia and peripheral neuropathy are the most prevalent adverse events, which are likely driven by the payload MMAE and instability of the maleimide conjugation chemistry.TUB-010 uses the same antibody brentuximab and payload MMAE as Adcetris, but instead of maleimide chemistry, TUB-010 is based on the Tub-tag conjugation strategy, which enables the generation of a homogenous and site-specific DAR 2 ADC with unique biophysical properties. This new technology stably attaches MMAE to the hydrophilic Tub-tag peptides on the light chains via chemoenzymatic conjugation using the enzyme tubulin tyrosine ligase.TUB-010 demonstrates similar binding affinity, internalization and lysosomal release characteristics as Adcetris in CD30-positive cells. When normalized to the MMAE concentration, TUB-010 shows comparablein vitrocytotoxic efficacy as well as similar bystander activity compared to Adcetris on established cancer cell lines. Importantly, TUB-010 exhibits higher stability with neglectable premature deconjugation in circulation and reduced high molecular weight species formation as well as lower non-specific cytotoxicity on target-negative cells compared to Adcetris. As a consequence, TUB-010 induces superior tumor control compared to Adcetris when dosed at equal MMAE concentrationsin vivoand also shows lower toxicity and higher tolerability in rodents and non-human primates.Taken together, TUB-010 is a novel and potential best-in-class anti-CD30 ADC with improved biophysical properties designed to deliver the cytotoxic payload with higher precision and with a wider therapeutic window than Adcetris using Tub-tag conjugation technology. Therefore, TUB-010 may increase the clinical benefit of ADC therapies for patients with CD30-positive malignancies.