bluuebunny/crossref_metadata_2025_split huggingface dataset:
Patient-derived zebrafish xenografts of uveal melanoma reveal ferroptosis as a drug target
Authors: Groenewoud Arwin , Yin Jie , Gelmi Maria Chiara , Alsafadi Samar , Nemati Fariba , Decaudin Didier , Roman-Roman Sergio , Kalirai Helen , Coupland Sarah E. , Jochemsen Aart G. , Jager Martine J. , Engel Felix B. , Snaar-Jagalska B. E. 
Published: June 2023

AbstractUveal melanoma (UM) has a high risk to progress to metastatic disease with a median survival of 3.9 months after metastases detection, as metastatic UM responds poorly to conventional and targeted chemotherapy and is largely refractory to immunotherapy. Here, we present a patient-derived zebrafish UM xenograft model mimicking metastatic UM. Cells isolated from Xmm66 spheroids derived from metastatic UM patient material were injected into 2 days-old zebrafish larvae resulting in micro-metastases in the liver and caudal hematopoietic tissue. Metastasis formation could be reduced by navitoclax and more efficiently by the combinations navitoclax/everolimus and flavopiridol/quisinostat. We obtained spheroid cultures from 14 metastatic and 10 primary UM tissues, which were used for xenografts with a success rate of 100%. Importantly, the ferroptosis-related genes GPX4 and SLC7A11 are negatively correlated with the survival of UM patients (TCGA: n = 80; Leiden University Medical Centre cohort: n = 64), ferroptosis susceptibility is correlated with loss of BAP1, one of the key prognosticators for metastatic UM, and ferroptosis induction greatly reduced metastasis formation in the UM xenograft model. Collectively, we have established a patient-derived animal model for metastatic UM and identified ferroptosis induction as a possible therapeutic strategy for the treatment of UM patients.
Potential cross-species transmission of highly pathogenic avian influenza H5 subtype (HPAI H5) viruses to humans calls for the development of H5-specific and universal influenza vaccines
Authors: Huang Pan , Sun Lujia , Li Jinhao , Wu Qingyi , Rezaei Nima , Jiang Shibo , Pan Chungen 
Published: June 2023

AbstractIn recent years, highly pathogenic avian influenza H5 subtype (HPAI H5) viruses have been prevalent around the world in both avian and mammalian species, causing serious economic losses to farmers. HPAI H5 infections of zoonotic origin also pose a threat to human health. Upon evaluating the global distribution of HPAI H5 viruses from 2019 to 2022, we found that the dominant strain of HPAI H5 rapidly changed from H5N8 to H5N1. A comparison of HA sequences from human- and avian-derived HPAI H5 viruses indicated high homology within the same subtype of viruses. Moreover, amino acid residues 137A, 192I, and 193R in the receptor-binding domain of HA1 were the key mutation sites for human infection in the current HPAI H5 subtype viruses. The recent rapid transmission of H5N1 HPAI in minks may result in the further evolution of the virus in mammals, thereby causing cross-species transmission to humans in the near future. This potential cross-species transmission calls for the development of an H5-specific influenza vaccine, as well as a universal influenza vaccine able to provide protection against a broad range of influenza strains.
Do radiomics or diffusion-tensor images provide additional information to predict brain amyloid-beta positivity?
Authors: Jo Sungyang , Lee Hyunna , Kim Hyung-Ji , Suh Chong Hyun , Kim Sang Joon , Lee Yoojin , Roh Jee Hoon , Lee Jae-Hong 
Published: June 2023

AbstractThe aim of the present study was to predict amyloid-beta positivity using a conventional T1-weighted image, radiomics, and a diffusion-tensor image obtained by magnetic resonance imaging (MRI). We included 186 patients with mild cognitive impairment (MCI) who underwent Florbetaben positron emission tomography (PET), MRI (three-dimensional T1-weighted and diffusion-tensor images), and neuropsychological tests at the Asan Medical Center. We developed a stepwise machine learning algorithm using demographics, T1 MRI features (volume, cortical thickness and radiomics), and diffusion-tensor image to distinguish amyloid-beta positivity on Florbetaben PET. We compared the performance of each algorithm based on the MRI features used. The study population included 72 patients with MCI in the amyloid-beta-negative group and 114 patients with MCI in the amyloid-beta-positive group. The machine learning algorithm using T1 volume performed better than that using only clinical information (mean area under the curve [AUC]: 0.73 vs. 0.69, p < 0.001). The machine learning algorithm using T1 volume showed better performance than that using cortical thickness (mean AUC: 0.73 vs. 0.68, p < 0.001) or texture (mean AUC: 0.73 vs. 0.71, p = 0.002). The performance of the machine learning algorithm using fractional anisotropy in addition to T1 volume was not better than that using T1 volume alone (mean AUC: 0.73 vs. 0.73, p = 0.60). Among MRI features, T1 volume was the best predictor of amyloid PET positivity. Radiomics or diffusion-tensor images did not provide additional benefits.
Evaluation of inactivation of bovine coronavirus by low-level radiofrequency irradiation
Authors: Cantu Jody C. , Butterworth Joseph W. , Mylacraine Kevin S. , Ibey Bennett L. , Gamboa Bryan M. , Johnson Leland R. , Thomas Robert J. , Payne Jason A. , Roach William P. , Echchgadda Ibtissam 
Published: June 2023

AbstractInactivation of influenza A virus by radiofrequency (RF) energy exposure at levels near Institute of Electrical and Electronics Engineers (IEEE) safety thresholds has been reported. The authors hypothesized that this inactivation was through a structure-resonant energy transfer mechanism. If this hypothesis is confirmed, such a technology could be used to prevent transmission of virus in occupied public spaces where RF irradiation of surfaces could be performed at scale. The present study aims to both replicate and expand the previous work by investigating the neutralization of bovine coronavirus (BCoV), a surrogate of SARS-CoV-2, by RF radiation in 6–12 GHz range. Results showed an appreciable reduction in BCoV infectivity (up to 77%) due to RF exposure to certain frequencies, but failed to generate enough reduction to be considered clinically significant.
Multi-parental fungal mapping population study to detect genomic regions associated with Pyrenophora teres f. teres virulence
Authors: Dahanayaka Buddhika A. , Martin Anke 
Published: June 2023

AbstractIn recent years multi-parental mapping populations (MPPs) have been widely adopted in many crops to detect quantitative trait loci (QTLs) as this method can compensate for the limitations of QTL analyses using bi-parental mapping populations. Here we report the first multi-parental nested association mapping (MP-NAM) population study used to detect genomic regions associated with host-pathogenic interactions. MP-NAM QTL analyses were conducted on 399 Pyrenophora teres f. teres individuals using biallelic, cross-specific and parental QTL effect models. A bi-parental QTL mapping study was also conducted to compare the power of QTL detection between bi-parental and MP-NAM populations. Using MP-NAM with 399 individuals detected a maximum of eight QTLs with a single QTL effect model whilst only a maximum of five QTLs were detected with an individual bi-parental mapping population of 100 individuals. When reducing the number of isolates in the MP-NAM to 200 individuals the number of QTLs detected remained the same for the MP-NAM population. This study confirms that MPPs such as MP-NAM populations can be successfully used in detecting QTLs in haploid fungal pathogens and that the power of QTL detection with MPPs is greater than with bi-parental mapping populations.
Home range ecology of Indian rock pythons (Python molurus) in Sathyamangalam and Mudumalai Tiger Reserves, Tamil Nadu, Southern India
Authors: Vishnu C. S. , Marshall Benjamin Michael , Ramesh Chinnasamy , Thirumurugan Vedagiri , Talukdar Gautam , Das Abhijit 
Published: June 2023

AbstractThe Indian rock pythons (Python molurus) are classified as a near-threatened snake species by the International Union for the Conservation of Nature and Natural Resources (IUCN); they are native to the Indian subcontinent and have experienced population declines caused primarily by poaching and habitat loss. We hand-captured the 14 rock pythons from villages, agricultural lands, and core forests to examine the species' home ranges. We later released/translocated them in different kilometer ranges at the Tiger Reserves. From December 2018 to December 2020, we obtained 401 radio-telemetry locations, with an average tracking duration of (444 ± 212 days), and a mean of 29 ± SD 16 data points per individual. We quantified home ranges and measured morphometric and ecological factors (sex, body size, and location) associated with intraspecific differences in home range size. We analyzed the home ranges of rock pythons using Auto correlated Kernel Density Estimates (AKDE). AKDEs can account for the auto-correlated nature of animal movement data and mitigate against biases stemming from inconsistent tracking time lags. Home range size varied from 1.4 ha to 8.1 km2 and averaged 4.2 km2. Differences in home range sizes could not be connected to body mass. Initial indications suggest that rock python home ranges are larger than other pythons.
Many-body quantum muon effects and quadrupolar coupling in solids
Authors: Gomilšek Matjaž , Pratt Francis L. , Cottrell Stephen P. , Clark Stewart J. , Lancaster Tom 
Published: June 2023

AbstractStrong quantum zero-point motion (ZPM) of light nuclei and other particles is a crucial aspect of many state-of-the-art quantum materials. However, it has only recently begun to be explored from an ab initio perspective, through several competing approximations. Here we develop a unified description of muon and light nucleus ZPM and establish the regimes of anharmonicity and positional quantum entanglement where different approximation schemes apply. Via density functional theory and path-integral molecular dynamics simulations we demonstrate that in solid nitrogen, α–N2, muon ZPM is both strongly anharmonic and many-body in character, with the muon forming an extended electric-dipole polaron around a central, quantum-entangled [N2–μ–N2]+ complex. By combining this quantitative description of quantum muon ZPM with precision muon quadrupolar level-crossing resonance experiments, we independently determine the static 14N nuclear quadrupolar coupling constant of pristine α–N2 to be –5.36(2) MHz, a significant improvement in accuracy over the previously-accepted value of –5.39(5) MHz, and a validation of our unified description of light-particle ZPM.
Solar-like oscillations inγCephei A as seen through SONG and TESS
Authors: Knudstrup E. , Lund M. N. , Fredslund Andersen M. , Rørsted J. L. , Pérez Hernández F. , Grundahl F. , Pallé P. L. , Stello D. , White T. R. , Kjeldsen H. , Vrard M. , Winther M. L. , Handberg R. , Simón-Díaz S. 
Published: June 2023

Context.Fundamental stellar parameters such as mass and radius are some of the most important building blocks in astronomy, both when it comes to understanding the star itself and when deriving the properties of any exoplanet(s) they may host. Asteroseismology of solar-like oscillations allows us to determine these parameters with high precision.Aims.We investigate the solar-like oscillations of the red-giant-branch starγCep A, which harbours a giant planet on a wide orbit.Methods.We did this by utilising both ground-based radial velocities from the SONG network and space-borne photometry from the NASA TESS mission.Results.From the radial velocities and photometric observations, we created a combined power spectrum, which we used in an asteroseismic analysis to extract individual frequencies. We clearly identify several radial and quadrupole modes as well as multiple mixed, dipole modes. We used these frequencies along with spectroscopic and astrometric constraints to model the star, and we find a mass of 1.27−0.07+0.05M⊙, a radius of 4.74−0.08+0.07R⊙, and an age of 5.7−0.9+0.8Gyr. We then used the mass ofγCep A and our SONG radial velocities to derive masses forγCep B andγCep Ab of 0.328−0.012+0.009M⊙and 6.6−2.8+2.3 MJup, respectively.
An Update on Impacts of Epigallocatechin Gallate Co-administration in Modulating Pharmacokinetics of Statins, Calcium Channel Blockers, and Beta-blockers
Authors: Siew-Keah Lee , Jie Tan Hong , Ang-Lim Chua , Bin Liew Kai , Yik-Ling Chew 
Published: June 2023

AbstractBrewed green tea, green tea extract, and its primary active compound, epigallocatechin gallate, may interact with drugs and alter the drugʼs therapeutic effectiveness, ultimately leading to
therapeutic failure or drug overdose. Several isolated reports have claimed that epigallocatechin gallate is the main active ingredient that causes these effects. While a few studies aimed
to uncover evidence of epigallocatechin gallate-drug interactions, no study has thoroughly and collectively reviewed them. Epigallocatechin gallate is a potential cardioprotective agent used
by many patients with cardiovascular diseases as a complementary medicine alongside conventional modern medications, either with or without the knowledge of their physicians. Therefore, this
review focuses on the impact of concurrent epigallocatechin gallate supplementation on pharmacokinetics and pharmacodynamics of several commonly used cardiovascular drugs (statins,
beta-blockers, and calcium channel blockers). The PubMed index was searched for key words related to this review, without year limit, and the results were analyzed for interactions of
cardiovascular drugs with epigallocatechin gallate. This review concludes that epigallocatechin gallate increases systemic circulation of several statins (simvastatin, fluvastatin,
rosuvastatin) and calcium channel blockers (verapamil), but decreases the bioavailability of beta-blockers (nadolol, atenolol, bisoprolol). Further studies on its clinical significance in
affecting drug efficacy are required.
Techniques Providing Endpoints for Revascularization in Chronic Limb-Threatening Ischemia
Authors: Kutsenko Oleksandra , Sommerset Jill , Chandra Venita , Bryce Yolanda 
Published: June 2023

AbstractIt is frequently difficult to estimate the revascularization endpoint in patients with chronic limb-threatening ischemia where there may be extensive multifocal multiarterial disease. There have been attempts to identify an endpoint for revascularization procedures, but none has become the standard of care. An ideal indicator of an endpoint can objectively quantify tissue perfusion, predict wound healing, and is easily and efficiently used intraprocedurally to assist real-time decision making on whether adequate perfusion has been reached. Candidate techniques to evaluate endpoints post-revascularization are discussed here.
Post-sterilization Dimensional Accuracy of Methacrylate Monomer Biocompatible Three-Dimensionally Printed Mock Surgical Guides
Authors: Marturello Danielle M. , Déjardin Loïc M. 
Published: June 2023

Abstract
Objectives The aim of this study was to evaluate the post-sterilization dimensional accuracy of a standardized drilling guide, three-dimensionally printed using biocompatible methacrylate monomers.
Study Design A mock surgical guide was designed and printed in five resins (n = 5/material) using a commercially available desktop stereolithography printer. Pre- and post-sterilization dimensions were measured for each sterilization method (steam, ethylene oxide, hydrogen peroxide gas), then statistically compared; p-value less than or equal 0.05 was considered significant.
Results While all resins produced highly accurate replicas of the designed guide, the amber and black resins were unaffected by any sterilization method (p ≥ 0.9). For other materials, ethylene oxide produced the largest dimensional changes. However, mean post-sterilization dimensional changes for all materials and sterilization methods remained less than or equal to 0.05mm
Conclusion This study demonstrated that post-sterilization dimensional change of evaluated biomaterials was minimal, and less than previously reported. Additionally, amber and black resins may be preferred to reduce post-sterilization dimensional change, as they were unaffected by any sterilization method. Given the results of this study, surgeons should feel confident using the Form 3B printer to create patient surgical guides. Furthermore, bioresins may provide safer alternatives for patients compared with other three-dimensional printed materials.
Right Aortic Arch with Bicarotid Trunk and Isolated Left Subclavian Artery: Hitherto Unreported Pattern
Authors: Sharma Arun , Garg Dollphy , Naganur Sanjeev Hanumantacharya , Singhal Manphool 
Published: June 2023

AbstractVariant arch anatomy may be seen associated with many congenital heart diseases. Its accurate preoperative identification is of paramount importance in optimal surgical planning of such cases. This case describes one such variant arch anatomy with two vessel right aortic arch, comprising of bicarotid trunk (giving rise to bilateral common carotid arteries) and right subclavian artery with isolation of the left subclavian artery, in a patient with tetralogy of Fallot. Right aortic arch with isolated left subclavian artery has already been described in association with tetralogy of Fallot. However, to the best of our knowledge, present arch pattern consisting of right aortic arch with bicarotid trunk and isolated left subclavian artery has not been reported in literature so far. Moreover, this case highlights the utility of multidetector computed tomography in accurate identification of variant arch anatomy in addition to delineation of cardiac and extracardiac details.
National Early Warning Score-2 for Identification of Patients with Intermediate-High-Risk Pulmonary Embolism
Authors: Rodríguez Carmen , Muriel Alfonso , Carrasco Luis , González Sara , Briceño Winnifer , Durán Diego , Retegui Ana , Yusen Roger D. , Bikdeli Behnood , Jiménez David 
Published: June 2023

AbstractConsensus statements have proposed the use of the National Early Warning Score 2 (NEWS2) to identify stable patients with acute pulmonary embolism (PE) and an intermediate-high risk of adverse outcomes. We aimed to externally validate NEWS2 and compare it to another predictive score (Bova). Using NEWS2 (cutoff ≥5 and ≥7) and the Bova score (cutoff >4), we classified patients as intermediate-high risk (vs. non-intermediate-high risk), and we compared the test characteristics of these risk classification tools for a complicated course within 30 days after PE diagnosis. We also assessed the validity of NEWS2 for predicting a complicated course by adding the results of echocardiography and troponin testing to the model. Of the 848 enrolled patients, the NEWS2 score ≥5 classified 471 (55.5%) and the Bova score classified 37 (4.4%) as intermediate-high risk. NEWS2 had a significantly lower specificity for a 30-day complicated course than Bova (45.4 vs. 96.3%, respectively; p < 0.001). Using the higher score threshold (≥7), the NEWS2 classified 99 (11.7%) as intermediate-high risk, and the specificity was 88.9% (difference with Bova, 7.4%; p < 0.001). The proportion of patients with intermediate-high risk PE was 2.4% for the combination of a positive troponin testing and echocardiographic right ventricle dysfunction and a positive NEWS2 (score ≥7), while the specificity was 97.8% (difference with Bova, 1.5%; p = 0.07). Bova outperforms NEWS2 for predicting a complicated course among stable patients with PE. Addition of troponin testing and echocardiography improved the specificity of NEWS2, although it was not superior to Bova.
ClinicalTrials.gov number: NCT02238639.
Medical Management of Peripheral Artery Disease
Authors: Sullivan Alexander E. , Beckman Joshua A. 
Published: June 2023

AbstractPeripheral artery disease (PAD) is a common type of atherosclerotic disease of the lower extremities associated with reduced quality of life and ambulatory capacity. Major adverse cardiovascular events and limb amputations are the leading cause of morbidity and mortality in this population. Optimal medical therapy is therefore critical in these patients to prevent adverse events. Risk factor modifications, including blood pressure control and smoking cessation, in addition to antithrombotic agents, peripheral vasodilators, and supervised exercise therapy are key pillars of medical therapy. Revascularization procedures represent key touch points between patients and health care providers and serve as opportunities to optimize medical therapy and improve long-term patency rates and outcomes. This review summarizes the aspects of medical therapy that all providers should be familiar with when caring for patients with PAD in the peri-revascularization period.
Basics of Guidewire Technology and Peripheral Artery Disease
Authors: Tummala Srini , Patel Mike 
Published: June 2023

AbstractGuidewire technology has advanced significantly over the last several decades. As more components are incorporated providing valuable features, deciding which guidewire to use during peripheral artery disease (PAD) interventions has become more complex. The challenge for both the beginner and expert is not only understanding which components offer the best characteristics in a guidewire but choosing the optimal wire for an intervention. Manufacturers have attempted to optimize components to provide physicians with routinely available guidewires needed in everyday practice. Yet selecting the best guidewire for a particular situation during an intervention is still challenging. This article provides a basic overview of guidewire components and what benefits they offer during PAD interventions.
Design and construction of new small gradient coils for mouse MRI
Authors: Ahmadi H R , Mohammadzadeh M , Abbasi A 
Published: June 2023

Abstract
We represent small Z- and Y-gradient coils with higher gradient strength and slew rate for mouse MRI. The coil geometries are optimized based on predetermined conditions for the gradient strength and slew rate in terms of minimum power consumption and acceptable linearity for the variation of the magnetic fields using the particle swarm optimization algorithm in computer simulation technology. The simulation results verify that the magnitudes of the field strength and slew rate have reached up to

144

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1297

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for the Z-gradient coil and increased to

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for the Y-gradient coil, respectively. Furthermore, the optimal geometry of the Z-gradient coil derived from a simulation study was then fabricated, and its parameters were measured using a Hall sensor. The gradient strength and slew rate of the fabricated Z-gradient coil were measured at

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, respectively, which have only a 9% difference from the corresponding simulated parameters.