bluuebunny/crossref_metadata_2025_split huggingface dataset:
Gain-enhanced triple-band rectifier-integrated antenna for microwave wireless power transmission
Authors: Polaiah Geriki 
Published: September 2023

AbstractThis paper proposes a triple-band fractal slot antenna with enhanced gain operating at frequencies of 2.7, 3.5, and 4.8 GHz for microwave wireless power transmission. The antenna achieved more than 3 dB gain by preserving the symmetrical defected reflector structure behind it at a length of 0.54λ/4, where λ is the wavelength in free space at 2.7 GHz. A 50 Ω micro-strip line coupled at 66 mm length helps feed the triple band slot antenna. The purpose of a triple-band rectifier circuit with an impedance network is to convert the radio frequency (RF) signal into direct current. The measurements reveal that the antenna’s enhanced gain is 8.8 dB, and the rectifier’s highest RF signal to direct current conversion efficiency is 74.3%. For the combined configuration unit of measure, the antenna is assimilated into the rectifier via back-to-back 50 Ω Sub Miniature Version A (SMA) connectors. The highest recorded efficiency of 49.67% was obtained for the rectenna at optimum values of 1 kΩ resistive load and −5 dBm power input. It has been revealed that the rectifier-integrated antenna presented is effective for low-input RF energy capture and power transmission.
Smoking, tobacco dependence, and neurometabolites in the dorsal anterior cingulate cortex
Authors: O’Neill Joseph , Diaz Maylen Perez , Alger Jeffry R. , Pochon Jean-Baptiste , Ghahremani Dara , Dean Andrew C. , Tyndale Rachel F. , Petersen Nicole , Marohnic Shane , Karaiskaki Andrea , London Edythe D. 
Published: September 2023

AbstractCigarette smoking has a major impact on global health and morbidity, and positron emission tomographic research has provided evidence for reduced inflammation in the human brain associated with cigarette smoking. Given the consequences of inflammatory dysfunction for health, the question of whether cigarette smoking affects neuroinflammation warrants further investigation. The goal of this project therefore was to validate and extend evidence of hypoinflammation related to smoking, and to examine the potential contribution of inflammation to clinical features of smoking. Using magnetic resonance spectroscopy, we measured levels of neurometabolites that are putative neuroinflammatory markers. N-acetyl compounds (N-acetylaspartate + N-acetylaspartylglutamate), glutamate, creatine, choline-compounds (phosphocholine + glycerophosphocholine), and myo-inositol, have all been linked to neuroinflammation, but they have not been examined as such with respect to smoking. We tested whether people who smoke cigarettes have brain levels of these metabolites consistent with decreased neuroinflammation, and whether clinical features of smoking are associated with levels of these metabolites. The dorsal anterior cingulate cortex was chosen as the region-of-interest because of previous evidence linking it to smoking and related states. Fifty-four adults who smoked daily maintained overnight smoking abstinence before testing and were compared with 37 nonsmoking participants. Among the smoking participants, we tested for associations of metabolite levels with tobacco dependence, smoking history, craving, and withdrawal. Levels of N-acetyl compounds and glutamate were higher, whereas levels of creatine and choline compounds were lower in the smoking group as compared with the nonsmoking group. In the smoking group, glutamate and creatine levels correlated negatively with tobacco dependence, and creatine correlated negatively with lifetime smoking, but none of the metabolite levels correlated with craving or withdrawal. The findings indicate a link between smoking and a hypoinflammatory state in the brain, specifically in the dorsal anterior cingulate cortex. Smoking may thereby increase vulnerability to infection and brain injury.
ANO7 African-ancestral genomic diversity and advanced prostate cancer
Authors: Jiang Jue , Soh Pamela X. Y. , Mutambirwa Shingai B. A. , Bornman M. S. Riana , Haiman Christopher A. , Hayes Vanessa M. , Jaratlerdsiri Weerachai 
Published: September 2023

Abstract
Background
Prostate cancer (PCa) is a significant health burden for African men, with mortality rates more than double global averages. The prostate specific Anoctamin 7 (ANO7) gene linked with poor patient outcomes has recently been identified as the target for an African-specific protein-truncating PCa-risk allele.

Methods
Here we determined the role of ANO7 in a study of 889 men from southern Africa, leveraging exomic genotyping array PCa case-control data (n = 780, 17 ANO7 alleles) and deep sequenced whole genome data for germline and tumour ANO7 interrogation (n = 109), while providing clinicopathologically matched European-derived sequence data comparative analyses (n = 57). Associated predicted deleterious variants (PDVs) were further assessed for impact using computational protein structure analysis.

Results
Notably rare in European patients, we found the common African PDV p.Ile740Leu (rs74804606) to be associated with PCa risk in our case-control analysis (Wilcoxon rank-sum test, false discovery rate/FDR = 0.03), while sequencing revealed co-occurrence with the recently reported African-specific deleterious risk variant p.Ser914* (rs60985508). Additional findings included a novel protein-truncating African-specific frameshift variant p.Asp789Leu, African-relevant PDVs associated with altered protein structure at Ca2+ binding sites, early-onset PCa associated with PDVs and germline structural variants in Africans (Linear regression models, −6.42 years, 95% CI = −10.68 to −2.16, P-value = 0.003) and ANO7 as an inter-chromosomal PCa-related gene fusion partner in African derived tumours.

Conclusions
Here we provide not only validation for ANO7 as an African-relevant protein-altering PCa-risk locus, but additional evidence for a role of inherited and acquired ANO7 variance in the observed phenotypic heterogeneity and African-ancestral health disparity.
Prognostic signature based on mitochondria quality control proteins for the prediction of lung adenocarcinoma patients survival
Authors: Gorbunova Anna S. , Zamaraev Alexey V. , Yapryntseva Maria A. , Kovaleva Olga V. , Tchevkina Elena M. , Turkina Maria V. , Zhivotovsky Boris , Kopeina Gelina S. 
Published: September 2023

AbstractLung cancer is the leading cause of cancer mortality worldwide. In recent years, the incidence of lung cancer subtype lung adenocarcinoma (LUAD) has steadily increased. Mitochondria, as a pivotal site of cell bioenergetics, metabolism, cell signaling, and cell death, are often dysregulated in lung cancer cells. Mitochondria maintenance and integrity depend on mitochondrial quality control proteins (MQCPs). During lung cancer progression, the levels of MQCPs could change and promote cancer cell adaptation to the microenvironment and stresses. Here, univariate and multivariate proportional Cox regression analyses were applied to develop a signature based on the level of MQCPs (dimeric form of BNIP3, DRP1, and SIRT3) in tumorous and non-tumorous samples of 80 patients with LUAD. The MQCP signature could be used to separate the patients with LUAD into high- and low-risk groups. Survival analysis indicated that patients in the high-risk group had dramatically shorter overall survival compared with the low-risk patients. Moreover, a nomogram combining clinicopathologic features and the MQCP signature was constructed and validated to predict 1-, 3-, and 5-year overall survival of the patients. Thus, this study presents a novel signature based on MQCPs as a reliable prognostic tool to predict overall survival for patients with LUAD.
When less is more: shortening the Lpp protein leads to increased vancomycin resistance in Escherichia coli
Authors: Wykes Hannah , Le Vuong Van Hung , Olivera Catrina , Rakonjac Jasna 
Published: September 2023

AbstractVancomycin is a naturally occurring cell-wall-targeting glycopeptide antibiotic. Due to the low potency of this antibiotic against Gram-negative pathogens, such as Escherichia coli, there is a limited knowledge about interactions between vancomycin and this group of bacteria. Here, we show that an in-frame 63 bp deletion of the lpp gene caused a fourfold increase in vancomycin resistance in E. coli. The resulting protein, LppΔ21, is 21 amino acids shorter than the wild-type Lpp, a helical structural lipoprotein that controls the width of the periplasmic space through its length. The mutant remains susceptible to synergistic growth inhibition by combination of furazolidone and vancomycin; with furazolidone decreasing the vancomycin MIC by eightfold. These findings have clinical relevance, given that the vancomycin concentration required to select the lpp mutation is reachable during typical vancomycin oral administration for treating Clostridioides difficile infections. Combination therapy with furazolidone, however, is likely to prevent emergence and outgrowth of the lpp-mutated Gram-negative coliforms, avoiding exacerbation of the patient’s condition during the treatment.
Realizing unipolar and bipolar intrinsic skyrmions in MXenes from high-fidelity first-principles calculations
Authors: Kabiraj Arnab , Mahapatra Santanu 
Published: September 2023

AbstractMagnetic skyrmions, which are topologically protected tiny spin textures, have emerged as information carriers in energy-efficient logic and memory devices. Skyrmions are commonly realized by inducing large Dzyaloshinskii–Moriya interaction (DMI) in the interface of heavy metal heterolayers. With the advent of two-dimensional magnetism, it is being envisioned to host intrinsic skyrmions in a monolayer, which will be free from any interfacial defect and stacking order. Here using high-fidelity exchange-correlation functional-based first-principles calculations, we investigate such a possibility in methodically designed non-centrosymmetric MXene structures. From a search space of about 3000 materials, our customized high-throughput computational pipeline systematically harnesses out-of-the-plane and in-plane magnetism along with strong DMI to realize typical ‘unipolar’ skyrmions in 78 materials and exotic ‘bipolar’ skyrmions in 13 materials. Micromagnetic and atomistic Monte Carlo simulations further reveal that skyrmions in some of these materials may be stable at room temperature without any external magnetic field. Our study may pave the way for the practical realization of skyrmions-based information technology.
Local human impacts disrupt depth-dependent zonation of tropical reef fish communities
Authors: Richardson Laura E. , Heenan Adel , Delargy Adam J. , Neubauer Philipp , Lecky Joey , Gove Jamison M. , Green J. A. Mattias , Kindinger Tye L. , Ingeman Kurt E. , Williams Gareth J. 
Published: September 2023

AbstractThe influence of depth and associated gradients in light, nutrients and plankton on the ecological organization of tropical reef communities was first described over six decades ago but remains untested across broad geographies. During this time humans have become the dominant driver of planetary change, requiring that we revisit historic ecological paradigms to ensure they capture the dynamics of contemporary ecological systems. Analysing >5,500 in-water reef fish surveys between 0 and 30 m depth on reef slopes of 35 islands across the Pacific, we assess whether a depth gradient consistently predicts variation in reef fish biomass. We reveal predictable ecological organization at unpopulated locations, with increased biomass of planktivores and piscivores and decreased primary consumer biomass with increasing depth. Bathymetric steepness also had a striking influence on biomass patterns, primarily for planktivores, emphasizing potential links between local hydrodynamics and the upslope propagation of pelagic subsidies to the shallows. However, signals of resource-driven change in fish biomass with depth were altered or lost for populated islands, probably due to depleted fish biomass baselines. While principles of depth zonation broadly held, our findings expose limitations of the paradigm for predicting ecological dynamics where human impacts confound connections between ecological communities and their surrounding environment.
Single-cell lineage capture across genomic modalities with CellTag-multi reveals fate-specific gene regulatory changes
Authors: Jindal Kunal , Adil Mohd Tayyab , Yamaguchi Naoto , Yang Xue , Wang Helen C. , Kamimoto Kenji , Rivera-Gonzalez Guillermo C. , Morris Samantha A. 
Published: September 2023

AbstractComplex gene regulatory mechanisms underlie differentiation and reprogramming. Contemporary single-cell lineage-tracing (scLT) methods use expressed, heritable DNA barcodes to combine cell lineage readout with single-cell transcriptomics. However, reliance on transcriptional profiling limits adaptation to other single-cell assays. With CellTag-multi, we present an approach that enables direct capture of heritable random barcodes expressed as polyadenylated transcripts, in both single-cell RNA sequencing and single-cell Assay for Transposase Accessible Chromatin using sequencing assays, allowing for independent clonal tracking of transcriptional and epigenomic cell states. We validate CellTag-multi to characterize progenitor cell lineage priming during mouse hematopoiesis. Additionally, in direct reprogramming of fibroblasts to endoderm progenitors, we identify core regulatory programs underlying on-target and off-target fates. Furthermore, we reveal the transcription factor Zfp281 as a regulator of reprogramming outcome, biasing cells toward an off-target mesenchymal fate. Our results establish CellTag-multi as a lineage-tracing method compatible with multiple single-cell modalities and demonstrate its utility in revealing fate-specifying gene regulatory changes across diverse paradigms of differentiation and reprogramming.
Newborn and child-like molecular signatures in older adults stem from TCR shifts across human lifespan
Authors: van de Sandt Carolien E. , Nguyen Thi H. O. , Gherardin Nicholas A. , Crawford Jeremy Chase , Samir Jerome , Minervina Anastasia A. , Pogorelyy Mikhail V. , Rizzetto Simone , Szeto Christopher , Kaur Jasveen , Ranson Nicole , Sonda Sabrina , Harper Alice , Redmond Samuel J. , McQuilten Hayley A. , Menon Tejas , Sant Sneha , Jia Xiaoxiao , Pedrina Kate , Karapanagiotidis Theo , Cain Natalie , Nicholson Suellen , Chen Zhenjun , Lim Ratana , Clemens E. Bridie , Eltahla Auda , La Gruta Nicole L. , Crowe Jane , Lappas Martha , Rossjohn Jamie , Godfrey Dale I. , Thomas Paul G. , Gras Stephanie , Flanagan Katie L. , Luciani Fabio , Kedzierska Katherine 
Published: September 2023

AbstractCD8+ T cells provide robust antiviral immunity, but how epitope-specific T cells evolve across the human lifespan is unclear. Here we defined CD8+ T cell immunity directed at the prominent influenza epitope HLA-A*02:01-M158–66 (A2/M158) across four age groups at phenotypic, transcriptomic, clonal and functional levels. We identify a linear differentiation trajectory from newborns to children then adults, followed by divergence and a clonal reset in older adults. Gene profiles in older adults closely resemble those of newborns and children, despite being clonally distinct. Only child-derived and adult-derived A2/M158+CD8+ T cells had the potential to differentiate into highly cytotoxic epitope-specific CD8+ T cells, which was linked to highly functional public T cell receptor (TCR)αβ signatures. Suboptimal TCRαβ signatures in older adults led to less proliferation, polyfunctionality, avidity and recognition of peptide mutants, although displayed no signs of exhaustion. These data suggest that priming T cells at different stages of life might greatly affect CD8+ T cell responses toward viral infections.
Selection of cross-reactive T cells by commensal and food-derived yeasts drives cytotoxic TH1 cell responses in Crohn’s disease
Authors: Martini Gabriela Rios , Tikhonova Ekaterina , Rosati Elisa , DeCelie Meghan Bialt , Sievers Laura Katharina , Tran Florian , Lessing Matthias , Bergfeld Arne , Hinz Sophia , Nikolaus Susanna , Kümpers Julia , Matysiak Anna , Hofmann Philipp , Saggau Carina , Schneiders Stephan , Kamps Ann-Kristin , Jacobs Gunnar , Lieb Wolfgang , Maul Jochen , Siegmund Britta , Seegers Barbara , Hinrichsen Holger , Oberg Hans-Heinrich , Wesch Daniela , Bereswill Stefan , Heimesaat Markus M. , Rupp Jan , Kniemeyer Olaf , Brakhage Axel A. , Brunke Sascha , Hube Bernhard , Aden Konrad , Franke Andre , Iliev Iliyan D. , Scheffold Alexander , Schreiber Stefan , Bacher Petra 
Published: September 2023

AbstractAberrant CD4+ T cell reactivity against intestinal microorganisms is considered to drive mucosal inflammation in inflammatory bowel diseases. The disease-relevant microbial species and the corresponding microorganism-specific, pathogenic T cell phenotypes remain largely unknown. In the present study, we identified common gut commensal and food-derived yeasts, as direct activators of altered CD4+ T cell reactions in patients with Crohn’s disease (CD). Yeast-responsive CD4+ T cells in CD display a cytotoxic T helper cell (TH1 cell) phenotype and show selective expansion of T cell clones that are highly cross-reactive to several commensal, as well as food-derived, fungal species. This indicates cross-reactive T cell selection by repeated encounter with conserved fungal antigens in the context of chronic intestinal disease. Our results highlighted a role of yeasts as drivers of aberrant CD4+ T cell reactivity in patients with CD and suggest that both gut-resident fungal commensals and daily dietary intake of yeasts might contribute to chronic activation of inflammatory CD4+ T cell responses in patients with CD.
Investigating the role of bulking agents in compost maturity
Authors: Zahra Khadija , Farhan Muhammad , Kanwal Amina , Sharif Faiza , Hayyat Muhammad Umar , Shahzad Laila , Ghafoor Gul Zareen 
Published: September 2023

AbstractKitchen waste is increasing globally, similarly in Pakistan bulk of municipal solid waste comprises of kitchen waste specifically, tea waste. Composting of kitchen waste is one of the promising ways to convert waste into useful product, resulting into zero waste. This study is aimed to convert waste (kitchen waste) in to a resource (compost) using bulking agents (tea waste and biochar) for reducing maturity time. Secondly, compost application on Solanum lycopersicum (tomato) was also tested. Four compost treatments were designed under aerobic composting conditions for 30 days. Tea waste and biochar have accelerated the maturity rate and produced a nutrient rich compost. Final compost had Electrical Conductivity of 2mS/cm, Carbon Nitrogen ration of 15, 54% of organic matter, 15% of moisture content, 48% of cellulose content, and 28% of Lignin content. With the use of Co-compost the Solanum lycopersicum showed 133% germination index, 100% germination, 235% Munoo-Liisa Vitality Index and 1238% seed vigor index. Co-compost also improved the soil total nitrogen by 1.4%, total phosphorous by 2%, total potassium by 2.1% and bulk density by 2.6 gcm−3. This study successfully used tea waste and biochar as bulking agents to reduce maturation time to 30 days. Tea waste and biochar enhanced the organic matter degradation, lignocellulose degradation, water holding capacity, porosity, seed’s vigor, germination index. This research can be helpful in developing home composting and home gardening to combat solid waste management and food security issue in developing countries.
Isolation and characterization of bacterial cellulose produced from soybean whey and soybean hydrolyzate
Authors: Liu Xin , Cao Liang , Wang Shenao , Huang Li , Zhang Yu , Tian Miaoyi , Li Xuejiao , Zhang Jinyou 
Published: September 2023

AbstractSoybean whey and soybean hydrolyzate can be used for the biotechnological production of high-value products. Herein, we isolate soybean whey (SW)-and soybean hydrolyzate (SH)-derived bacterial cellulose (BC, produced by kombucha) and characterize it by a range of instrumental techniques to reveal differences in micromorphology, crystallinity, and themal behavior. Studies have shown that the amounts of wet state BC produced from HS, SW and SH was 181 g/L, 47 g/L and 83 g/L, respectively. The instrumental analysis of BC, included SEM, AFM, FT-IR, XRD and TGA. It is shown that the FT-IR spectra of BC have a similar character, but we found differences in the micromorphology,crystallinity and thermal temperature of BC. The minimum average widths of the fibers produced from SH medium was 100 ± 29 nm. The CrI values of BC produced from SH medium was 61.8%. The maximum thermal degradation rate temperature of BC produced from SW medium was 355.73 °C. The combined results demonstrate that soybean industrial waste can be used as a cost-effective raw material for BC production.
Machine learning-based optimal crop selection system in smart agriculture
Authors: Rani Sita , Mishra Amit Kumar , Kataria Aman , Mallik Saurav , Qin Hong 
Published: September 2023

AbstractThe cultivation of most crops depends upon the regional weather conditions. So, the analysis of the agro-climatic conditions of a zone contributes significantly to deciding the right crop for the right land in the right season to obtain a better yield. Machine learning algorithms facilitate this process to a great extent for better results. In this paper, the authors proposed an ML-based crop selection model based on the weather conditions and soil parameters, collectively. Weather analysis is done using LSTM RNN and the process of crop selection is completed using Random Forest Classifier. This model gives better results for weather prediction in comparison to ANN. With LSTM RNN, the RMSE observed in Min. Temp. prediction is 5.023%, Max. Temp. Prediction is 7.28%, and Rainfall Prediction is 8.24%. In the second phase, the Random Forest Classifier showed 97.235% accuracy for crop selection, 96.437% accuracy in predicting resource dependency, and 97.647 accuracies in giving the appropriate sowing time for the crop. The model construction time taken with a random forest classifier using mentioned data size is 5.34 s. The authors also suggested the future research direction to further improve this work.