bluuebunny/crossref_metadata_2025_split huggingface dataset:
Temporal transcriptomic dynamics in developing macaque neocortex
Authors: Xu Longjiang , Yuan Zan , Zhou Jiafeng , Zhao Yuan , Liu Wei , Lu Shuaiyao , He Zhanlong , Qiang Boqin , Shu Pengcheng , Chen Yang , Peng Xiaozhong 
Published: July 2023

AbstractDespite intense research on mice, the transcriptional regulation of neocortical neurogenesis remains limited in humans and non-human primates. Cortical development in rhesus macaque is known to recapitulate multiple facets of cortical development in humans, including the complex composition of neural stem cells and the thicker supragranular layer. To characterize temporal shifts in transcriptomic programming responsible for differentiation from stem cells to neurons, we sampled parietal lobes of rhesus macaque at E40, E50, E70, E80, and E90, spanning the full period of prenatal neurogenesis. Single-cell RNA sequencing produced a transcriptomic atlas of the developing rhesus macaque neocortex parietal lobe. Identification of distinct cell types and neural stem cells emerging in different developmental stages revealed a terminally bifurcating trajectory from stem cells to neurons. Notably, deep-layer neurons appear in the early stages of neurogenesis, while upper-layer neurons appear later. While these different lineages show overlap in their differentiation program, cell fates are determined post-mitotically. Pseudotime trajectories from vRGs to oRGs revealed differences in dynamic gene expression profiles and identified divergence in their activation of BMP, FGF, and WNT signaling pathways. These results provide a comprehensive picture of the temporal patterns of gene expression leading to different fates of radial glial progenitors during neocortex layer formation.
Prostaglandins as Candidate Ligands for a Per-ARNT-Sim (PAS) Domain of Steroid Receptor Coactivator 1 (SRC1)
Authors: Daffern Nicolas , Kelley Kade , Villegas José A. , Radhakrishnan Ishwar 
Published: July 2023

AbstractSteroid receptor coactivators (SRCs) comprise a family of three paralogous proteins commonly recruited by eukaryotic transcription factors. Each SRC harbors two tandem Per-ARNT-Sim (PAS) domains that are broadly distributed that bind small molecules and regulate interactions. Using computational docking, solution NMR, mass spectrometry, and molecular dynamics simulations, we show that the SRC1 PAS-B domain can bind to certain prostaglandins (PGs) either non-covalently to a surface that overlaps with the site used to engage transcription factors or covalently to a single, specific, conserved cysteine residue next to a solvent accessible hydrophobic pocket. This pocket is in proximity to the canonical transcription factor binding site, but on the opposite side of the domain, suggesting a potential mode of regulating transcriptional activator-coactivator interactions.
Elucidating the functional profiles of cultivable bacterial communities in the water and sediments during the southwest monsoon season, along the west coast of India
Authors: Parab Ashutosh S , Ghose Mayukhmita , Manohar Cathrine S 
Published: July 2023

AbstractUnderstanding the functional profiles of cultivable bacterial communities is crucial for comprehending their ecological significance in marine environments. This study investigates the functional profiles of cultivable bacterial communities in the water and sediments during the productive, southwest monsoon season, along the west coast of India. The southwest monsoon plays a vital role in shaping the hydrological and biogeochemical characteristics of this region, making it an ideal period to study bacterial communities and their functions. Our study utilizes a cultivation techniques to study taxonomy as well as functional assays to elucidate the diverse organic substrates utilization capabilities of bacterial communities. Cultivable bacteria were isolated from discrete water depths and sediment samples from the coastal and off-shore region. Subsequently the carbohydrase, lipase and protease assays were performed to assess its functional potential. The results of this study reveal a rich diversity of cultivable bacterial communities including, representative from Proteobacteria, Firmicutes and Actinobacterial phylum with diverse functional profiles. The functional analyses provide insights into the metabolic capabilities of the bacteria, including organic substrates degradation, processes. The bacterial taxonomic diversity and enzymes activities were significantly different (p < 0.001) among the water and sediment bacterial morphotypes. In conclusion, this research sheds light on the functional profiles of cultivable bacterial communities in the water and sediments along the west coast of India during the productive southwest monsoon season. The comprehensive analysis of their functional capabilities provides insights into their ecological roles and potential significance in organic matter recycling. These findings contribute to our understanding of microbial diversity and ecosystem functioning in coastal environments and lay the groundwork for further research on harnessing the significance of these bacteria in biogeochemical cycling.
Intercalation of the disulfide bond between the A2 and A4 loop of Cellobiohydrolase (Cel7A) ofAspergillus fumigatusenhances catalytic activity and thermostability
Authors: Dodda Subba Reddy , Hossain Musaddique , Mondal Sudipa , Das Shalini , Khator (Jain) Sneha , Aikat Kaustav , Mukhopadhyay Sudit S. 
Published: July 2023

AbstractDisulfide bond is important for maintaining the structural conformation and stability of the protein. Introducing new disulfide bond is a promising strategy for rational protein design. In this report, disulfide bond engineering has been applied to improve the stability of an industrially important enzyme, Glycoside Hydrolase family GH 7 cellobiohydrolase (GH7 CBHs) or Cel7A ofA fumigatusorigin. Disulfide by Design 2.0 (DbD2), an online tool, was used for the detection of the mutation sites and created four mutations (D276C-G279C; DSB1, D322C-G327C; DSB2, T416C-I432C; DSB3, G460C-S465C; DSB4) both inside and outside of the peripheral loops but, not in the catalytic region. The disulfide bond formed between the A2 and A4 loop of DSB3 showed higher thermostability (70% activity at 700C), higher substrate affinity (Km= 0.081mM) and higher catalytic activity (Kcat=9.75 min-1; Kcat/Km= 120.37 mM min-1) compared to wild typeAfCel7A (50% activity at 700C; Km= 0.128mM; Kcat= 4.833 min-1; Kcat/Km= 37.75 mM min-1). The other three mutants with high B factor showed loss of thermostability and catalytic activity. Molecular dynamic simulations revealed that the mutation T416C-I432C makes the tunnel wider (DSB3:13.6 Å; Wt: 5.3 Å) at the product exit site; giving flexibility in the entrance region and mobility of the substrate. It may facilitate substrate entry into the catalytic tunnel and releases the product faster than the wild type. Whereas in other mutants, the tunnel is not prominent (DSB4), the exit is lost (DSB1), and the ligand binding site is absent (DSB2). This is the first report of the gain of function of both thermostability and enzyme activity of cellobiohydrolase Cel7A by disulfide bond engineering in the loop.ImportanceCellulases are important for their role in the production of bioethanol, the cleanest renewable replacement of fossil fuels. Engineering of the cellulases is a chalange to increase their catalytic activity and thermostability for production of cheap ethanol. In this report we have introduced disulfide bond and successfully increased the both thermostabilty and catalytic activity ofAfCel7A.
The transcription factor OCT6 promotes the dissolution of the naïve pluripotent state by repressingNanogand activating a formative state gene regulatory network
Authors: Waisman Ariel , Sevlever Federico , Saulnier Denisse , Francia Marcos , Lombardi Antonella , Biani Celeste , Palma María Belén , Scarafía Agustina , Smucler Joaquín , Amín Guadalupe , Greca Alejandro La , Moro Lucía , Sevlever Gustavo , Guberman Alejandra , Miriuka Santiago 
Published: July 2023

AbstractAnimal development relies on complex gene regulatory networks (GRNs) that govern the nearly irreversible changes that occur during cell differentiation. In this work we aimed to determine key transcription factors (TFs) associated with the dissolution of the naïve pluripotent state and the acquisition of a formative identity. We identified OCT6 as one of the earliest TFs induced during the onset of mouse embryonic stem cell (mESCs) differentiation. To investigate its role, we generated anOct6knockout mESC line, which failed to acquire the characteristic cell morphology associated with the formative state. Transcriptome analysis of differentiating cells revealed nearly 300 differentially expressed genes compared to wild-type cells, including pluripotency TFsNanog, Klf2, Nr5a2, Prdm14,andEsrrb, that failed to correctly downregulate. Notably, premature expression of OCT6 in naïve cells triggered a rapid morphological transformation mirroring differentiation, accompanied by self-induction of Oct6 and expression of TFs such asSox3, Zic2/3, Foxp1, as well as the formative genesDnmt3AandFGF5. Strikingly, the majority of OCT6 expressing cells did not express NANOG. Gene expression and single molecule RNA-FISH analysis confirmed that this regulation was at the transcriptional level. Collectively, our results establish OCT6 as a key TF in the dissolution of the naïve pluripotent state and support a model whereOct6andNanogform a double negative feedback loop which could act as a toggle switch important for the transition to the formative state.HighlightsOct6is rapidly induced as mESCs exit ground state pluripotency.Loss of OCT6 negatively affects the transition to formative pluripotency.Premature expression of OCT6 in mESCs is sufficient to induce a formative-like phenotype.OCT6 and NANOG repress each other forming a double negative feedback loop.
Food at your doorstep? Examining customer loyalty towards online food delivery applications
Authors: Liu Xin , Lim Xin-Jean , Cheah Jun-Hwa , Ng Siew Imm , Kamal Basha Norazlyn 
Published: July 2023

PurposeThis study empirically investigated the different attributes (i.e. convenience, online reviews, online ratings, visual appeal and various food choices) of food delivery applications (FDAs) that impacted customers' perceived benefits, satisfaction and loyalty. Price consciousness was positioned as the moderator while customer satisfaction was positioned as the mediator.Design/methodology/approachUtilizing the purposive sampling technique, 360 useable respondents were gathered from existing FDAs' customers in China. Partial least squares structural equation modelling (PLS-SEM) was employed to verify the interrelationships among variables.FindingsThe results confirmed the significant impact of four FDA attributes (i.e. convenience, online ratings, visual appeal and various food choices) on perceived benefits. Customer satisfaction positively influenced loyalty. Moreover, the mediating role of customer satisfaction and the moderating role of price consciousness were confirmed.Practical implicationsFDA operators should engage in providing more benefits to stimulate satisfaction and loyalty among customers. Promotion discounts remain a key factor in enhancing the satisfaction and loyalty of price-conscious customers.Originality/valueThis study extends the applicability of the stimulus–organism–response (SOR) framework and enriches FDA literature by assessing the external stimuli, organisms and moderating factors that trigger customer loyalty.
EcoTrend: new line of the ecological toilet in the Peruvian market
Authors: Regalado-Pezua Otto , Horna-Saldaña César Jhonnatan , Toro Leonardo 
Published: July 2023

Learning outcomes
The learning outcomes of the study are to analyze the launch of a new business line for Trend at the commercial and market levels; identify the potential of the green consumer in Peruvian emerging market; and apply strategic tools to analyze the viability of launching a new business line in a new market.

Case overview/synopsis
José Luis Galindo planned to launch a new line of toilets in the Peruvian market called EcoTrend, based on the analysis of the responsible consumption trend and the presence of a new green consumer. Therefore, he carried out a series of studies and estimates to define the feasibility of the value proposition of his ecological toilet. However, Galindo doubted if these studies and estimates were enough to carry out the launch and commercial success of the EcoTrend line. Galindo, founder and current general manager of a company called Cerámica Industrial Trend S.A.C, is broadly knowledgeable about the construction sector in Peru and has more than 30 years of work experience in the ceramic bathroom fixtures industry. Throughout his professional career, Galindo has managed three of the leading bathroom fixture companies in Peru. However, it was when he started Trend, a company focusing specifically on the manufacture of toilets, that his dream of becoming an entrepreneur came true. Trend is focused on its one-piece toilet line. These toilets are characterized by their high-quality workmanship, which is achieved through the efficient and distinctive production process of Trend’s workforce. The workforce stays on its toes due to constant, thorough training, a key to Trend’s market competitiveness. In addition, the new EcoTrend line sowed in Galindo uncertainty in the commercial viability because the product was new in the market and was going to bring a great challenge.

Complexity academic level
Depending on the scope of the course, different teaching objectives could be oriented toward entrepreneurship, management sciences, strategy and green marketing. The case can be used to teach higher level undergraduate marketing and management courses.

Supplementary materials
Teaching notes are available for educators only.

Subject code
CSS 8: Marketing.
Implementing corporate sustainability information in socially responsible investing: a systematic review of empirical research
Authors: Daugaard Dan , Jia Jing , Li Zhongtian 
Published: July 2023

PurposeThis study aims to provide a precise understanding of how corporate sustainability information is used in socially responsible investing (SRI). The study is motivated by the lack of a recognised body of knowledge on this issue. This study, therefore, collates and reviews relevant studies (67 studies) to provide guidance to investors interested in SRI and identify a research agenda for academics desiring to contribute to this area.Design/methodology/approachThis study conducts a systemic literature review employing recognised key words and searching the Web of Science. HistCite is utilised to ensure important cited studies are not missed from the collection. The review was conducted from two perspectives: (1) sources of sustainability information and (2) how the information is used in SRI.FindingsThe review identifies five major sources of sustainability information, including corporate reports, ESG ratings, industry affiliation, news and private communication with firms. These sources of information play different roles in the cross section of SRI strategies (i.e. negative and positive screening, active ownership and integration). This study provides guidance on how to use this information in SRI and provides recommendations for future research on how analysts interact with the information, how different informational characteristics impact implementation, ways to improve data quality, improvements to analysis methods and where data use needs to be extended into new strategies.Originality/valueThis review contributes to the SRI literature by inventorying studies of an important, yet omitted aspect, namely, sustainability information. This work also enriches the literature on corporate sustainability information by investigating how this information can be used for a specific purpose, namely, SRI. Given the increasing interest in SRI, this review will provide much-needed guidance for a range of practitioners, including investors and regulators.
Investigating the impact of the dynamics of entrepreneurial intentions on ventures’ formalization
Authors: Yana Mbena Jacques , Durst Susanne , Kraus Sascha , Viala Céline 
Published: July 2023

Purpose
To overcome economic hardship and survival, informal entrepreneurs must continually activate specific resilience capabilities and reassess their entrepreneurial intentions (EI). Accordingly, they may develop transformational characteristics to design a model supporting intersectorial business growth. This study aims to examine the EI ecosystem of change resulting from the above endeavors and proposes an entrepreneurial intention transformation model (EITM).

Design/methodology/approach
Using the EI questionnaire developed by Liñán and Chen, data was collected from street vendors in the main markets of the Cameroonian capital (N = 307) to capture the determinants of behavior reflecting the propensity to activate entrepreneurial events within informal ventures.

Findings
It was uncovered that perceived collective support, resilience, orientation capability and inferred policies influence the dynamics of EI in the informal sector. Additionally, while revisiting current entrepreneurial lifecycle models, the study distinguished between the entrepreneurial events of “renegare” and formalization. Furthermore, the construction of an informal entrepreneurial lifecycle model confirms the need to acknowledge the formalization and “deformalization” processes as integrative parts of an intersectorial entrepreneurial lifecycle model.

Originality/value
This study extends existing research by proposing an intersectorial entrepreneurial lifecycle to recognize the dynamics of EI on ventures’ formalization. The EITM encompasses the EI dynamic process model and the proposed entrepreneurial lifecycle.
A factor analysis of landlords’ and retail trade tenants’ different beliefs in lease negotiations: evidence from the Swedish market
Authors: Lundgren Berndt Allan , Hermansson Cecilia , Gyllenberg Filip , Koppfeldt Johan 
Published: July 2023

Purpose The purpose is to increase knowledge of rent negotiations by investigating differences in beliefs held by property landlords and retailers on factors that they deem important in rent negotiation.Design/methodology/approach This study investigates differences in subjective beliefs held by landlords and retail trade tenants on factors that affect rent levels during the rent negotiation process using a factor analysis approach. Semi-structured interviews were made with seven large real estate owners/landlords and retailers and eight experts in negotiating retail rent to elicit variables that have an impact on retail rent. Thereafter, a web-based survey was sent to 421 respondents who had experience in rent negotiation. Several factors were extracted using factor analysis. The data collection was made in Sweden during the coronavirus disease 2019 (COVID-19) pandemic in late spring 2021Findings Significant differences are found in beliefs held by landlords and retail trade tenants in four out of seven-factor: regional growth, e-commerce, customer focus and trust. Landlords rate these factors higher than retailers do. There are also systematic differences between landlords and retailers depending on their education levels on the following factors: rent and vacancies, e-commerce and customer focus. The number of years of experience did not prove to be significant instead differences are found to exist in factorsResearch limitations/implications Not only do traditional factors of importance, such as lease structure, the effect of location, size and anchor or non-anchor tenants, have an effect on negotiated rent levels. Differences in other factors also exist, such as regional growth, e-commerce, customer focus and trust factors that may play an important role in the negation of retail rent.Practical implications The findings provide new insights into the different views on factors that affect rent negotiations between landlords and retail tenants. Knowledge of such differences may increase the overall transparency in the negotiation process. Transparency may be increased by putting forward information on these factors before a negotiation takes place, in order to smooth differences in their beliefs.Social implications If transparency in the negotiation process of retail rent increases, time to reach an agreement, stress and anxiety can be reduced by putting forward information on factors where differences exist between landlords and retailersOriginality/value New insights on retail rent negotiation have been put forward in this research paper. Not only do traditional factors such as lease structure matters, but subjective beliefs on factors such as regional growth and the level of education are also important, as this study has shown using a factors analysis approach.
Lying in online social networks: a bug or a feature
Authors: Maddah Mahed , Esmaeilzadeh Pouyan 
Published: July 2023

PurposeOnline social networks can bridge the gap between distant individuals by simulating online experiences that closely resemble physical interactions. While people have positive experiences, such as joy, in a physical relationship and would like to enjoy those experiences online, they also have negative experiences, such as being subject to a lie. An online social network may allow users to lie to simulate a real-world social group better. However, lying must be prevented on social networks as unethical behavior. Thus, this study aims to investigate an ethical dilemma raised due to the two abovementioned perspectives.Design/methodology/approachThis paper examines different aspects of lying in social networks, using a comprehensive descriptive literature review approach and a classification scheme.FindingsContrary to their initial intent, social networks are disrupting the traditional notion of human interaction, either by disregarding the presence of dishonesty or by attempting to regulate relationships. In this research, the authors examine the challenges of these two perspectives by identifying enablers, consequences and control measures of lying on social networks at the individual, social and technological levels. The authors also develop a framework to facilitate investigating this dilemma.Originality/valueThis paper examines a controversial topic. Although lying is unethical, allowing users to lie may be considered an appealing feature of social network platforms. This paper offers a framework to propose several research directions for future studies. This study’s examination focuses on the challenges associated with deception on social networks, prompting the formulation of three crucial questions regarding the potential impact of technology on such behavior.