bluuebunny/crossref_metadata_2025_split huggingface dataset:
Spatiotemporal Transcriptomic Profiling Reveals the Dynamic Immunological Landscape of Alveolar Echinococcosis
Authors: Ou Zhihua , Li Li , Ren Peidi , Zhou Ting‐Ting , He Fan , Chen Jialing , Cai Huimin , Han Xiumin , Wu Yao‐Dong , Li Jiandong , Li Xiu‐Rong , Tan Qiming , Li Wenhui , Chen Qi , Zhang Nian‐Zhang , He Xiuju , Chen Wei‐Gang , Zhao Yanping , Sun Jiwen , Zhang Qian , Wu Yan‐Tao , Liang Yingan , You Jie , Hu Guohai , Tian Xue‐Qi , Liao Sha , Fu Bao‐Quan , Chen Ao , Cai Xue‐Peng , Yang Huanming , Wang Jian , Jin Xin , Xu Xun , Jia Wan‐Zhong , Li Junhua , Yan Hong‐Bin 
Published: February 2025

AbstractAlveolar echinococcosis (AE) is caused by the chronic infection of E. multilocularis, whose tumor‐like growth can lead to high fatality if improperly treated. The early diagnosis of infection and the treatment of advanced AE remain challenging. Herein, bulk RNA‐seq, scRNA‐seq, and spatial transcriptomics technologies are integrated, to reveal the host immune response mechanism against E. multilocularis both spatially and chronologically, collecting mouse liver samples at multiple timepoints up to 15 months post infection. These results unveil an unprecedented high‐resolution spatial atlas of the E. multilocularis infection foci and the functional roles of neutrophils, Spp1+ macrophages, and fibroblasts during disease progression. The heterogeneity of neutrophil and macrophage subpopulations are critical in both parasite‐killing and the occurrence of immunosuppression during AE progression. These findings indicate the transition of parasite control strategy from “active killing” to “negative segregation” by the host, providing instructive insights into the treatment strategy for echinococcosis.
Control of Rhizobia Endosymbiosis by Coupling ER Expansion with Enhanced UPR
Authors: Ren Jing , Wang Qi , Zhang Xiaxia , Cao Yongheng , Wu JingXia , Tian Juan , Yu Yanjun , Gong Qingqiu , Kong Zhaosheng 
Published: February 2025

AbstractLegumes establish symbiosis with rhizobia by forming a symbiotic interface that enables cross‐kingdom exchanges of signaling molecules and nutrients. However, how host organelles interact with symbiosomes at the symbiotic interface remains elusive during rhizobia endosymbiosis. Here, symbiotic cells are reconstructed using 3D scanning electron microscopy (SEM) and uncover that the host endoplasmic reticulum (ER) undergoes dynamic expansion to gradually enwrap symbiosomes, facilitating their compartmentalization and endosymbiosis. Consistently, altering ER lamellar expansion by overexpressing MtRTNLBs, the reticulons responsible for ER tubulation, impairs rhizobia accommodation and symbiosome development. Intriguingly, unfolded protein response (UPR)‐marker genes, bZIP60 and IRE1A/B, show continuously activated expression during nodule development, and the two UPR‐deficient mutants, ire1b, and bzip60, exhibit compromised ER biogenesis and defective symbiosome development. Collectively, the findings underpin ER expansion and UPR activation as two key events in rhizobia accommodation and reveal an intrinsic coupling of ER morphology with proper UPR during root nodule symbiosis.
Polysilazane‐Coated Films Achieving Record‐High Moisture Barrier Performance with Sub‐10 Seconds Densification Using High‐Power VUV Irradiation
Authors: Song Luyang , Sun He , Suzuri Yoshiyuki 
Published: February 2025

AbstractAn ultra‐high moisture barrier compact SiNX film can be achieved from solution‐processed perhydropolysilazane (PHPS) through vacuum ultraviolet (VUV) light exposure. This study investigates the photochemical reactions and photo‐densification of PHPS‐based barrier films under varying VUV light intensities, focusing on their effects on barrier performance. Photo‐dehydrogenation of PHPS, involving N─H and Si─H bond cleavage, is efficient and unaffected by light intensity. However, photo‐densification shows a strong dependence on light intensity, particularly above 290 mW cm−2. Higher intensities enhance Si─N bond cleavage, alter film dynamics, and reduce free volume through bond rearrangement, facilitating rapid network reconstruction essential for ultra‐high barrier properties. High‐power VUV light at 309 mW cm−2 establishes a new benchmark for ultra‐high barrier films via solution processing, achieving a record‐low average water vapor transmission rate (WVTR) of 1.6 × 10−5 g m−2 day−1. Films are produced in under 10 s per layer, maintaining a barrier property of 3.8 × 10−5 g m−2 day−1. The optimal refractive index for the top 30 nm layer is 1.74–1.77, controlling WVTR within 10−5 g m−2 day−1, ensuring superior barrier performance for flexible electronic devices, such as perovskite solar cells and organic photovoltaics.
A Versatile Protein Scaffold Engineered for the Hierarchical Assembly of Robust and Highly Active Enzymes
Authors: Meng Yiwei , Peplowski Lukasz , Wu Tong , Gong Heng , Gu Ran , Han Laichuang , Xia Yuanyuan , Liu Zhongmei , Zhou Zhemin , Cheng Zhongyi 
Published: February 2025

AbstractScaffold proteins play immense roles in bringing enzymes together to enhance their properties. However, the direct fusion of scaffold with bulky guest enzymes may disrupt the assembly process or diminish catalytic efficiency. Most self‐assembling protein scaffolds are engineered to form structures beforehand, and then carry guest proteins via different conjugation strategies in vitro. Here, a robust self‐assembling scaffold is presented, engineered from Methanococcus jannaschii using disulfide bonds, which efficiently assembles bulky enzymes into higher‐order helices without additional chemistry or bio‐conjugation in vitro. When fused directly with monomeric Endo‐1,4‐beta‐xylanase A, the catalytic efficiency of the guest enzyme increased by 2.5 times with enhanced thermostability. Additionally, integrating the scaffold with the multimeric metalloenzyme nitrile hydratase overcame the typical stability‐activity trade‐off of such industrial enzyme, yielding three‐fold higher activity and 28‐fold higher thermostability. Structural analyses suggest that the artificially made helical twist structures create new interface interactions and provide a concentration of active sites of guest enzymes. Further fusion of fluorescent protein pairs with the scaffold exhibited a 12‐fold higher FRET efficiency, suggesting its potential for dual‐enzyme cascade applications. Overall, this study showcases a simple yet powerful protein scaffold that organizes guest enzymes into hierarchical structures with enhanced catalytic performance.
Machine Learning‐Driven Surrogate Modeling for Optimization of Triboelectric Nanogenerator Design Parameters
Authors: Uddin Mohammad Abrar , Lim Myeongju , Kim Rubiga , Burgess Barrett London , Roberts Ken , Kim Junghyun , Kim Taeil 
Published: February 2025

AbstractTriboelectric nanogenerators (TENGs) offer a promising solution for energy harvesting in wearable devices and sensors. However, their energy output is dependent on process parameters and should be optimized to maximize performance. Due to the absence of effective analytical models for TENG systems, the complex relationship among these variables and the effect of these variables cannot be easily boiled down into a conventional theoretical framework. To address this problem, this study takes four process parameters such as thickness, pore ratio, applied force, and frequency into account and leverages advanced design methods (e.g., Design of Experiment) and machine learning‐based regression models to systematically explore the design space. A contact‐separation TENG has been designed that includes a tribonegative porous layer of graphene nanoplatelets (GNP) dispersed into polydimethylsiloxane (PDMS) matrix and aluminum as the tribopositive material. Several experiments are conducted to train a support vector regressor (SVR) model, validate the predicted performance, and refine the design that can be further used to obtain an optimized TENG design.
Fine‐tuning coordination from WO to WN in graphene gel based single‐atom W for hydrogenation of p‐chloronitrobenzene
Authors: Cai An , Zhang Qicheng , Hou Fang , Li Guozhu , Xi Shibo , Fan Xiaobin , Peng Wenchao , Li Yang 
Published: February 2025

AbstractThe rational design of single‐atom catalysts (SACs) with controllable coordination environments is indispensable for various target reactions. It is still a challenge due to the difficulty in understanding atomic behavior specifically in complex synthetic processes. Notably, such deep research and structure–activity relationship of tungsten (W) SACs are still unclear because of its variable oxidation states and challenging synthesis. Herein, W single atoms anchored in three‐dimensional N‐doped graphene gel are synthesized through the combined strategy of spatial confinement and coordination design, dispensing with post‐treatment. Systematic research on structural evolution under various synthetic conditions is conducted. The investigation is put forward to uncover the structure–activity relationship toward chemoselective hydrogenation of p‐chloronitrobenzene. The relatively abundant active sites, WN/C coordination environment, absence of WO, and unique cross‐linking structure promote the reaction synergistically. The experimental results and theoretical calculations verify that the most efficient specific WN2C2‐E configuration addresses the trade‐off problem among activity, selectivity, and stability.
Durable Metal‐Organic Frameworks by Covalent Hybridization with Polyurethane and Polyallophanate
Authors: Anan Shizuka , Sada Kazuki , Kokado Kenta 
Published: February 2025

Metal‐organic frameworks (MOFs) are expected for have a multitude of applications due to their high porosity, crystallinity, and designability. However, the mechanical properties of MOFs are brittle due to the porosity and the weak coordination bond between the metal ions and the organic ligands. A method for enhancing the mechanical properties of MOFs while maintaining high porosity is anticipated to facilitate long‐term, stable utilization. In the present work, single crystals of MOFs are combined with polyurethanes inside pores of crystal using the crystal component‐linking method. Guest molecules of diisocyanates are introduced into the pores of MOFs composed of organic ligands with hydroxy groups, and the organic ligands are linked by the guest molecules via urethane bonds. The excess diisocyanates yielded allophanates, which function as crosslinking points, thereby forming polymer networks within the MOFs. The compressive fracture strength of MOFs combined with polyurethane and allophanate networks is observed to be approximately thirty times greater than that of MOFs without polymer networks. The covalent combination of rigid and fragile MOFs with flexible polymer networks is a promising approach for the improvement of the mechanical properties of MOFs.
Dentition of the Mugharet El'Aliya Fossil Human Maxilla, Morocco
Authors: Röding Carolin , El‐Zaatari Sireen , Ramirez Rozzi Fernando V. , Stringer Chris , Burgess M. Loring , Lacruz Rodrigo S. , Harvati Katerina 
Published: February 2025

ABSTRACTObjectiveThis study follows up on our recent morphological analysis of the juvenile maxilla from Mugharet el'Aliya, Morocco. Although this specimen shows a reportedly archaic morphology, likely due to its large size, 3D shape analyses indicated affinities with early Homo sapiens. Here, we conducted an in‐depth comparative investigation of the associated dentition to further clarify this individual's phylogenetic and taxonomic affinities.Materials and MethodsOur analyses were based on three kinds of data: (a) external crown dimensions and non‐metric features, analyzed via summary statistics; (b) CT scan data enabling the study of internal structures (enamel‐dentine junction) via geometric morphometrics; and (c) high‐resolution replicas of the external surface of the upper canine enabling the study of perikymata numbers via probability functions. The comparative samples included Middle Pleistocene (Chibanian) Europeans and Africans, Neanderthals, and early and later H. sapiens.ResultsMugharet el'Aliya showed the greatest similarities in external and internal tooth morphology with early and later H. sapiens. Perikymata counts cluster the upper canine with H. sapiens. However, its canine and fourth premolar are megadont at a level generally atypical for H. sapiens.DiscussionOur analyses of the dentition of the Mugharet el'Aliya individual support our previous findings on the morphological analysis of the maxilla, placing this fossil closest to H. sapiens. Our study further strengthens the evidence connecting fossils from the North African Aterian to those from Western Asia, especially Qafzeh. We also provide the first comparative analysis of a permanent upper canine from the Aterian fossil record.
SCD‐plus features and AD biomarkers in cognitively unimpaired samples: A meta‐analytic approach for nine cohort studies
Authors: Kuhn Elizabeth , Klinger Hannah M. , Amariglio Rebecca E. , Wagner Michael , Jessen Frank , Düzel Emrah , Heneka Michael T. , Chételat Gael , Rentz Dorene M. , Sperling Reisa A. , Ebenau Jarith L. , Butterbrod Elke , Van Der Flier Wiesje M. , Sikkes Sietske A. M. , Teunnissen Charlotte E. , Van Harten Argonde C. , Van De Giessen Elsmarieke M. , Rami Lorena , Tort Adria , Sánchez Benavides Gonzalo , Gifford Katherine A. , Van Hulle Carol , Buckley Rachel F. ,   Alzheimer's Disease Neuroimaging Initiative,   Australian Imaging Biomarkers and Lifestyle flagship study of ageing, A4 Study Team,   DELCODE Study,   Harvard Aging Brain Study
Published: February 2025

AbstractINTRODUCTIONSpecific features of subjective cognitive decline (SCD‐plus) have been proposed to indicate an increased risk of preclinical Alzheimer's disease (AD). However, few studies have examined how these features relate to AD biomarkers in cognitively unimpaired (CU) older adults.METHODSMeta‐analyses were performed using cross‐sectional data from nine cohorts (n = 7219, mean age (SD): 71.17 (5.9), 56.5% female) to determine associations of SCD‐plus features with positron emission tomography (PET)– or cerebrospinal fluid (CSF)–derived amyloid beta (Aβ) and tau biomarkers.RESULTSParticipants with preclinical AD (community‐based only) were more likely to fulfill SCD‐plus features. The presence of self‐reported memory decline, associated concern/worry, and a higher number of fulfilled features were all associated with high Aβ levels. Only the latter was associated with abnormal tau.DISCUSSIONSimultaneous endorsement of multiple SCD‐plus features is a robust indicator of abnormal AD biomarkers in CU older adults, whereas isolated SCD features seem only sensitive to elevated Aβ, supporting their value as early behavioral markers of preclinical AD.Highlights
About two‐tenths of our sample had abnormal amyloid beta (Aβ) levels with evidence of subjective cognitive decline (SCD).
Preclinical AD subsamples (community‐based) had a higher percentage of participants meeting SCD‐plus features. Self‐reported memory decline and concern/worry were the sole features associated with high Aβ, but not tau, burden.
A higher number of fulfilled SCD‐plus features are linked to high Aβ and tau burden.
Use of multiple SCD‐plus features may help identify early stages of biological AD.
Regional Cerebral Atrophy Contributes to Personalized Survival Prediction in Amyotrophic Lateral Sclerosis: A Multicentre, Machine Learning, Deformation‐Based Morphometry Study
Authors: Lajoie Isabelle ,   Canadian ALS Neuroimaging Consortium (CALSNIC), Kalra Sanjay , Dadar Mahsa 
Published: February 2025

ObjectiveAccurate personalized survival prediction in amyotrophic lateral sclerosis is essential for effective patient care planning. This study investigates whether grey and white matter changes measured by magnetic resonance imaging can improve individual survival predictions.MethodsWe analyzed data from 178 patients with amyotrophic lateral sclerosis and 166 healthy controls in the Canadian Amyotrophic Lateral Sclerosis Neuroimaging Consortium study. A voxel‐wise linear mixed‐effects model assessed disease‐related and survival‐related atrophy detected through deformation‐based morphometry, controlling for age, sex, and scanner variations. Additional linear mixed‐effects models explored associations between regional imaging and clinical measurements, and their associations with time to the composite outcome of death, tracheostomy, or permanent assisted ventilation. We evaluated whether incorporating imaging features alongside clinical data could improve the performance of an individual survival distribution model.ResultsDeformation‐based morphometry uncovered distinct voxel‐wise atrophy patterns linked to disease progression and survival, with many of these regional atrophies significantly associated with clinical manifestations of the disease. By integrating regional imaging features with clinical data, we observed a substantial enhancement in the performance of survival models across key metrics. Our analysis identified specific brain regions, such as the corpus callosum, rostral middle frontal gyrus, and thalamus, where atrophy predicted an increased risk of mortality.InterpretationThis study suggests that brain atrophy patterns measured by deformation‐based morphometry provide valuable insights beyond clinical assessments for prognosis. It offers a more comprehensive approach to prognosis and highlights brain regions involved in disease progression and survival, potentially leading to a better understanding of amyotrophic lateral sclerosis. ANN NEUROL 2025
Promoting Li+‐Solvents Desolvation by Engineering Nickel Single Atoms into Graphene Membrane toward Fast Sulfur Redox Kinetics
Authors: He Songjie , Yang Juan , Liu Zhibin , Liu Siyu , Yu Jiayi , Qiu Jieshan 
Published: February 2025

Lithium‐sulfur (Li‐S) batteries featuring high energy density are expected to be next‐generation energy storage devices, but are severely impeded by the suppressive Li+‐solvents desolvation process at the electrode/electrolyte interface. Herein, a novel electrochemical in‐situ doping coupled with self‐assembly strategy is proposed to fabricate the graphene membrane anchored by Ni single atoms (Ni‐SA‐G), aimed at promoting the dissociation kinetics of Li+‐solvents complex by combining electrocatalysis and nanochannel sieving effect. Theoretical simulation and in‐situ Raman spectroscopy characterizations revealed that the Ni‐O5 configuration within the Ni‐SA‐G membrane is capable of lowering the Li+‐solvent dissociation energy barrier and promoting free Li+ migration, thereby delivering the fast sulfur redox kinetics. In addition, taking advantage of the Ni‐SA‐G membrane with a special transport channel, the large‐sized solvent molecules and polysulfides were sieved and confined to a great degree. As a result, the Li‐S batteries with the Ni‐SA‐G as cathode front‐faces exhibit a high capacity of 1169 mAh g−1 with a good rate performance and outstanding long‐term cycling stability, where a capacity decay of only 0.024% per cycle after 700 cycles can be achieved. Furthermore, the cell with a sulfur loading of 4.78 mg cm−2 delivers a high areal capacity of 4.0 mAh cm−2 at 0.2 C.
Exploring of Antidepressant Components and Mechanisms of Zhizichi Decoction: Integration of Serum Pharmacochemistry, Network Pharmacology and Anti‐inflammatory Analysis Verification
Authors: Chai Chuan , Jin Bo , Bi Jinghan , Cui Yuhan , Cui Xiaobing , Shan Chenxiao , Yu Sheng , Wen Hongmei 
Published: February 2025

ABSTRACTUsing LC–MS to screen and analyse the characteristics of components and biological systems is a new approach to study the pharmacological substance basis of traditional Chinese medicine, which has strong novelty in analytical science. This study analyses the antidepressant material basis of Zhizichi decoction through the integration strategy of serum pharmacochemistry, network pharmacology and immunoreactivity verification, which helps in overcoming the limitations of TCM research and provided a new perspective and approach for studying the components of ZZCD. First of all, blood from SD rats was collected before and after Zhizichi decoction administration initially. The migration constituents in the serum were then analysed using ultra‐high performance liquid chromatography‐Q‐TOF‐MS/MS. By integrating the TCMSP databases with the serum pharmacochemistry results, we constructed the ‘ingredients–targets–pathways’ network and the protein–protein interaction network for Zhizichi decoction's depression‐relieving. Finally, the inhibitory effects of Zhizichi decoction and active ingredient groups comprised of pharmacodynamic components identified in prior network pharmacology study on IL‐1β, IL‐6 and TNF‐α were measured through an inflammatory cytokines experiments. From the serum pharmacochemistry study, 146 migration constituents in serum and their attribution were hypothesized and characterized. They were identified as 18 prototype components and 128 metabolites, of which 121 were Phase I and 7 were Phase II metabolites. The Zhizichi decoction pharmacology network illustrated the relationships of the 20 definitive ingredients, 85 potential targets and 21 signalling pathways in connection with the depression. The targets predicted by pharmacology and protein–protein interaction network were reported to be associated with neuroinflammation, which suggested that further anti‐inflammatory experiment was required. For the anti‐inflammatory effect of AIGs 1 composed of 14 pharmacodynamic components was basically equivalent to that of whole ZZCD recipe, AIG 1 was hypothesized to be the critical pharmacodynamic components to inhibit inflammatory factors and defined as the antidepressant components’ of Zhizichi decoction, providing a scientific foundation for the pursuit of potential new drugs for depression treatments.
Lanthanum(III) Coordination Polymer Decorated With 2,1,3‐Benzoxadiazole Units for Sensitive Luminescent Recognition of Sulfate Anion
Authors: Dudko Evgeny R. , Pavlov Dmitry I. , Ryadun Alexey A. , Guselnikova Tatiana Y. , Fedin Vladimir P. , Yu Xiaolin , Potapov Andrei S. 
Published: February 2025

ABSTRACTOne‐dimensional coordination polymer La‐CP, {[La(H2O)2(HLO) (LO)]·H2O}n containing a new luminescent ligand 4,7‐di(4‐carboxypyrazol‐1‐yl)‐2,1,3‐benzoxadiazole (H2LO) was synthesized. Upon excitation at 400 nm, the compound shows a strong emission with the maximum at 550 nm with high photoluminescence quantum yield of 43%. La‐CP demonstrates sensing properties toward aluminum and gallium cations, as well as dihydrogen phosphate and sulfate anions through the emission enhancement response. The limits of detection were 0.42, 0.70, 0.37, and 0.40 μM, respectively. La‐CP is the first example of a sensor for sulfate anion among all types of coordination polymers. The uptake of the analytes under the sensing conditions was evaluated, and a strong absorption enhancement as a result of such uptake was proposed as a probable emission response mechanism.