bluuebunny/crossref_metadata_2025_split huggingface dataset:
Characterisation of infection-induced SARS-CoV-2 seroprevalence amongst children and adolescents in North Carolina
Authors: Ahmed Amina , DeWitt Michael E. , Dantuluri Keerti L. , Castri Paola , Buahin Asare , LaGarde William H. , Weintraub William S. , Rossman Whitney , Santos Roberto P. , Gibbs Michael , Uschner Diane ,   for the COVID-19 Community Research Partnership
Published: April 2023

Abstract
Few prospective studies have documented the seropositivity among those children infected with severe acute respiratory syndrome coronavirus 2. From 2 April 2021 to 24 June 2021, we prospectively enrolled children between the ages of 2 and 17 years at three North Carolina healthcare systems. Participants received at least four at-home serological tests detecting the presence of antibodies against, but not differentiating between, the nucleocapsid or spike antigen. A total of 1,058 participants were enrolled in the study, completing 2,709 tests between 1 May 2021 and 31 October 2021. Using multilevel regression with poststratification techniques and considering our assay sensitivity and sensitivity, we estimated that the seroprevalence of infection-induced antibodies among unvaccinated children and adolescents aged 2–17 years in North Carolina increased from 15.2% (95% credible interval, CrI 9.0–22.0) in May 2021 to 54.1% (95% CrI 46.7–61.1) by October 2021, indicating an average infection-to-reported-case ratio of 5. A rapid rise in seropositivity was most pronounced in those unvaccinated children aged 12–17 years, based on our estimates. This study underlines the utility of serial, serological testing to inform a broader understanding of the regional immune landscape and spread of infection.
Association of cumulative prenatal adversity with infant subcortical structure volumes and child problem behavior and its moderation by a coexpression polygenic risk score of the serotonin system
Authors: Acosta Henriette , Kantojärvi Katri , Tuulari Jetro J. , Lewis John D. , Hashempour Niloofar , Scheinin Noora M. , Lehtola Satu J. , Nolvi Saara , Fonov Vladimir S. , Collins D. Louis , Evans Alan C. , Parkkola Riitta , Lähdesmäki Tuire , Saunavaara Jani , Merisaari Harri , Karlsson Linnea , Paunio Tiina , Karlsson Hasse 
Published: April 2023

AbstractPrenatal adversity has been linked to later psychopathology. Yet, research on cumulative prenatal adversity, as well as its interaction with offspring genotype, on brain and behavioral development is scarce. With this study, we aimed to address this gap. In Finnish mother–infant dyads, we investigated the association of a cumulative prenatal adversity sum score (PRE-AS) with (a) child emotional and behavioral problems assessed with the Strengths and Difficulties Questionnaire at 4 and 5 years (N = 1568, 45.3% female), (b) infant amygdalar and hippocampal volumes (subsample N = 122), and (c) its moderation by a hippocampal-specific coexpression polygenic risk score based on the serotonin transporter (SLC6A4) gene. We found that higher PRE-AS was linked to greater child emotional and behavioral problems at both time points, with partly stronger associations in boys than in girls. Higher PRE-AS was associated with larger bilateral infant amygdalar volumes in girls compared to boys, while no associations were found for hippocampal volumes. Further, hyperactivity/inattention in 4-year-old girls was related to both genotype and PRE-AS, the latter partially mediated by right amygdalar volumes as preliminary evidence suggests. Our study is the first to demonstrate a dose-dependent sexually dimorphic relationship between cumulative prenatal adversity and infant amygdalar volumes.
Fine lines: locating commercial sex work in official data, Dublin 1901 and 1911
Authors: Breathnach Ciara , Murphy Rachel 
Published: April 2023

AbstractDublin at the turn of the nineteenth century had limited permanent employment opportunities compared to Belfast, and for poor families financial instability manifested in limited life expectancy. This article focuses on young adult cohorts in Dublin city. By cross-referencing names and addresses from death records with census, court and prison records, it casts new light on the lives of the city's most disadvantaged people. It applies a digital humanities framework and uses historical Geographical Information Systems to explore patterns in cause of death, and to reveal more about household income, casual labour, women's work and community networks. We contend that the cautions about the occlusion of commercial sex work in historical data should be extended to the lowest strata of the working classes more generally and that it is only through granular analyses that the fine lines between poverty and destitution can emerge.
Experiences of parents of children with hypoplastic left heart syndrome during their treatment: a qualitative study
Authors: Silberberg Agustín , Castro Méndez Josefina , Torres Silvio F. , Fraire Rafael , Siaba Serrate Alejandro , Kreutzer Christian , López Daneri Mariana , Iolster Thomas 
Published: April 2023

AbstractAim:To investigate the opinion and personal experience of parents of children born with Hypoplastic Left Heart Syndrome and what advice they would give to other parents who have to decide between treatment options.Methods:We conducted a qualitative, descriptive and retrospective study by means of a survey directed to parents of children born with Hypoplastic Left Heart Syndrome in a tertiary hospital in Buenos Aires (Argentina). Their answers and data regarding medical procedures were analysed.Results:Parents of thirteen out of sixteen patients with Hypoplastic Left Heart Syndrome were surveyed. Norwood surgery had been performed in all the patients, many had received other procedures, and five had died. In relation to the decision-making process, sixty-one percent of parents would recommend other parents to remain at peace after having done everything possible and 54% would suggest to not feel guilt despite the final result. None of the parents would recommend rejecting surgical treatment and choosing comfort care.Conclusion:The majority of parents of children with Hypoplastic Left Heart Syndrome would recommend continuing with the therapeutic effort in order to feel at peace and reduce feelings of guilt.
Sulfur_X: A Model of Sulfur Degassing During Magma Ascent
Authors: Ding Shuo , Plank Terry , Wallace Paul J. , Rasmussen Daniel J. 
Published: April 2023

AbstractThe degassing of CO2 and S from arc volcanoes is fundamentally important to global climate, eruption forecasting, ore deposits, and the cycling of volatiles through subduction zones. However, all existing thermodynamic/empirical models have difficulties reproducing CO2‐H2O‐S trends observed in melt inclusions and provide widely conflicting results regarding the relationships between pressure and CO2/SO2 in the vapor. In this study, we develop an open‐source degassing model, Sulfur_X, to track the evolution of S, CO2, H2O, and redox states in melt and vapor in ascending mafic‐intermediate magma. Sulfur_X describes sulfur degassing by parameterizing experimentally derived sulfur partition coefficients for two equilibria: RxnI. FeS (m) + H2O (v) H2S (v) + FeO (m), and RxnII. CaSO4(m) SO2 (v) + O2 (v) + CaO (m), based on the sulfur speciation in the melt (m) and co‐existing vapor (v). Sulfur_X is also the first to track the evolution of fO2 and sulfur and iron redox states accurately in the system using electron balance and equilibrium calculations. Our results show that a typical H2O‐rich (4.5 wt.%) arc magma with high initial S6+/ΣS ratio (>0.5) will degas much more (∼2/3) of its initial sulfur at high pressures (>200 MPa) than H2O‐poor ocean island basalts with low initial S6+/ΣS ratio (<0.1), which will degas very little sulfur until shallow pressures (<50 MPa). The pressure‐S relationship in the melt predicted by Sulfur_X provides new insights into interpreting the CO2/ST ratio measured in high‐T volcanic gases in the run‐up to the eruption.
The Influence of Cloud Types on Cloud‐Radiative Forcing During DYNAMO/AMIE
Authors: Najarian Hrag , Sakaeda Naoko 
Published: April 2023

AbstractCloud‐radiative feedback is known to be an important process for the Madden‐Julian Oscillation (MJO) and its accurate representation in general circulation models. The MJO is known to have a stronger cloud‐radiative feedback compared to higher frequency convectively coupled equatorial waves (CCEW), for reasons that remain unclear. The objective of this study is to use data from the Dynamics of the MJO/Atmospheric Radiation Measurement MJO Investigation Experiment field campaign to investigate how cloud type evolution and their associated cloud‐radiative forcing (CRF) differ between the MJO and CCEWs and their application to MJO dynamics under moisture mode theory. This study finds that the amplitude of CRF is the largest within the MJO compared to the higher frequency CCEWs, and CRF maximizes after the maximum precipitation (enhanced phase) of the MJO. We suggest this delay in the timing of maximum CRF occurs because most cloud types (congestus, deep, anvil, and cirrus) simultaneously maximize in frequency after the enhanced phase of the MJO, which is not seen for the higher frequency CCEWs. These specific cloud types help maintain the MJO through their radiatively‐driven moistening which acts to prolong the enhanced phase of the MJO. The decomposition of radiatively‐driven moistening by cloud types shows that simultaneous moistening by deep, anvil, and congestus clouds are particularly responsible for the maintenance of the MJO under moisture mode theory. This analysis suggests the importance of capturing the correct evolution of different cloud types and their associated radiative effects to better understand the thermodynamics of the MJO.
Warming Overwhelms the Efficacy of Wet Conditions to Moderate Extreme Heat and Atmospheric Aridity Across the Central Plains
Authors: Chiang Felicia , Cook Benjamin I. , McDermid Sonali 
Published: April 2023

AbstractWhile the relationships between dry land surface conditions, heat, and aridity have been well‐established, few studies have addressed whether global warming will affect the ability of wet conditions to moderate high temperatures and atmospheric aridity. Using Coupled Model Intercomparison Project Phase 6 models, we demonstrate that absolute changes in the monthly maximum temperature distribution during Central North American summers strongly outweigh the historical cooling effect of high precipitation and soil moisture conditions. Although wet conditions nearly always prevent concurrent extreme temperatures in the baseline period, these conditions are 40%–48% and 96%–98% less effective at 1 and 2° of global warming, respectively. However, high precipitation and soil moisture partially retain the ability to constrain concurrent high vapor pressure deficit conditions below historical thresholds at 1–2° of warming. Our results highlight the growing vulnerability of Central North America to warmer temperatures and drier atmospheric conditions, even during periods of high precipitation and soil moisture.
Spatial Variations in Seamless Predictability of Subseasonal Precipitation Over Asian Summer Monsoon Region in S2S Models
Authors: Li Xiaojing , Tang Youmin , Shen Zheqi , Li Yi 
Published: April 2023

AbstractThis study assesses the seamless predictability of subseasonal precipitation over the Asian summer monsoon (ASM) region. The prediction skill of 12 models from the subseasonal‐to‐seasonal (S2S) hindcast database varies considerably, suggesting a large uncertainty in the prediction of ASM precipitation. However, all models show that ASM precipitation is better predicted over ocean than over land, and less reliable in subtropical areas than in tropical areas. The results reveal significant spatial variations in the prediction skill and predictability of ASM precipitation. This study investigates the factors controlling these spatial variations by analyzing the area‐averaged precipitation predictability in three different subregions: the maritime continent (MC), the Indian summer monsoon (ISM) subregion, and the East ASM (EASM) subregion. Precipitation is best predicted over the MC, followed by the ISM and EASM. The distinct disparities in prediction skill among subregions are mainly controlled by differences in potential predictability; that is, the intrinsic limits of predictability among subregions. The high potential predictability of the MC is attributable to small noise due to a strong correlation with the El Niño–Southern Oscillation, whereas the low potential predictability of the EASM is attributable to its small signal. Meanwhile, the comparable signal and noise related to the intraseasonal oscillation in the ISM precipitation, limits the predictability of this subregion. Finally, the evaluation of the ASM precipitation ensemble forecasts in the S2S models demonstrates that the ensemble systems are underdispersive in the three subregions in most S2S models.
TMPRSS4, a type II transmembrane serine protease, as a potential therapeutic target in cancer
Authors: Kim Semi 
Published: April 2023

AbstractProteases are involved in almost all biological processes, implying their importance for both health and pathological conditions. Dysregulation of proteases is a key event in cancer. Initially, research identified their role in invasion and metastasis, but more recent studies have shown that proteases are involved in all stages of cancer development and progression, both directly through proteolytic activity and indirectly via regulation of cellular signaling and functions. Over the past two decades, a novel subfamily of serine proteases called type II transmembrane serine proteases (TTSPs) has been identified. Many TTSPs are overexpressed by a variety of tumors and are potential novel markers of tumor development and progression; these TTSPs are possible molecular targets for anticancer therapeutics. The transmembrane protease serine 4 (TMPRSS4), a member of the TTSP family, is upregulated in pancreatic, colorectal, gastric, lung, thyroid, prostate, and several other cancers; indeed, elevated expression of TMPRSS4 often correlates with poor prognosis. Based on its broad expression profile in cancer, TMPRSS4 has been the focus of attention in anticancer research. This review summarizes up-to-date information regarding the expression, regulation, and clinical relevance of TMPRSS4, as well as its role in pathological contexts, particularly in cancer. It also provides a general overview of epithelial-mesenchymal transition and TTSPs.
Early activation of cellular stress and death pathways caused by cytoplasmic TDP-43 in the rNLS8 mouse model of ALS and FTD
Authors: Luan Wei , Wright Amanda L. , Brown-Wright Heledd , Le Sheng , San Gil Rebecca , Madrid San Martin Lidia , Ling Karen , Jafar-Nejad Paymaan , Rigo Frank , Walker Adam K. 
Published: April 2023

AbstractTAR DNA binding protein 43 (TDP-43) pathology is a key feature of over 95% of amyotrophic lateral sclerosis (ALS) and nearly half of frontotemporal dementia (FTD) cases. The pathogenic mechanisms of TDP-43 dysfunction are poorly understood, however, activation of cell stress pathways may contribute to pathogenesis. We, therefore, sought to identify which cell stress components are critical for driving disease onset and neurodegeneration in ALS and FTD. We studied the rNLS8 transgenic mouse model, which expresses human TDP-43 with a genetically-ablated nuclear localisation sequence within neurons of the brain and spinal cord resulting in cytoplasmic TDP-43 pathology and progressive motor dysfunction. Amongst numerous cell stress-related biological pathways profiled using qPCR arrays, several critical integrated stress response (ISR) effectors, including CCAAT/enhancer-binding homologous protein (Chop/Ddit3) and activating transcription factor 4 (Atf4), were upregulated in the cortex of rNLS8 mice prior to disease onset. This was accompanied by early up-regulation of anti-apoptotic gene Bcl2 and diverse pro-apoptotic genes including BH3-interacting domain death agonist (Bid). However, pro-apoptotic signalling predominated after onset of motor phenotypes. Notably, pro-apoptotic cleaved caspase-3 protein was elevated in the cortex of rNLS8 mice at later disease stages, suggesting that downstream activation of apoptosis drives neurodegeneration following failure of early protective responses. Unexpectedly, suppression of Chop in the brain and spinal cord using antisense oligonucleotide-mediated silencing had no effect on overall TDP-43 pathology or disease phenotypes in rNLS8 mice. Cytoplasmic TDP-43 accumulation therefore causes very early activation of ISR and both anti- and pro-apoptotic signalling that switches to predominant pro-apoptotic activation later in disease. These findings suggest that precise temporal modulation of cell stress and death pathways may be beneficial to protect against neurodegeneration in ALS and FTD.
Testosterone eliminates strategic prosocial behavior through impacting choice consistency in healthy males
Authors: Kutlikova Hana H. , Zhang Lei , Eisenegger Christoph , van Honk Jack , Lamm Claus 
Published: April 2023

AbstractHumans are strategically more prosocial when their actions are being watched by others than when they act alone. Using a psychopharmacogenetic approach, we investigated the endocrinological and computational mechanisms of such audience-driven prosociality. One hundred and ninety-two male participants received either a single dose of testosterone (150 mg) or a placebo and performed a prosocial and self-benefitting reinforcement learning task. Crucially, the task was performed either in private or when being watched. Rival theories suggest that the hormone might either diminish or strengthen audience-dependent prosociality. We show that exogenous testosterone fully eliminated strategic, i.e., feigned, prosociality and thus decreased submission to audience expectations. We next performed reinforcement-learning drift-diffusion computational modeling to elucidate which latent aspects of decision-making testosterone acted on. The modeling revealed that testosterone compared to placebo did not deteriorate reinforcement learning per se. Rather, when being watched, the hormone altered the degree to which the learned information on choice value translated to action selection. Taken together, our study provides novel evidence of testosterone’s effects on implicit reward processing, through which it counteracts conformity and deceptive reputation strategies.
Exercise intensity during exergaming in wheelchair-dependent persons with SCI
Authors: Wouda Matthijs Ferdinand , Gaupseth Jon-Arve , Bengtson Espen Ingvald. , Johansen Truls , Brembo Espen Andreas , Lundgaard Eivind 
Published: April 2023

Abstract
Study design
Cross-sectional study.

Objectives
It is challenging for persons with SCI, especially those who are wheelchair dependent, to find suitable and motivating aerobic exercise modes. Exergaming might be a good option, since it is relatively cheap and can be played at home, alone or with others. However, it is unknown if exergaming is performed at a sufficient exercise intensity.

Setting
Sunnaas Rehabilitation Hospital, Norway.

Methods
Twenty-two men and two women (n = 24) with chronic SCI (AIS A-C), all wheelchair users, were included during inpatient rehabilitation. All participant performed a maximal graded arm-crank test (pretest), while measuring peak oxygen uptake (VO2peak) and peak heart rate (HRpeak). The day after they had a practice playing session with three different exergames (X-box Kinect, Fruit Ninja; Nintendo Wii, Wii Sports Boxing; VR Oculus Rift, boxing). The following day, participants played each exergame for 15 min. During these 45 min of exergaming, exercise intensity, based on VO2peak and HRpeak from the pretest, was monitored.

Results
Approximately 30 of the 45 min of exergaming was performed at moderate or high intensity. Participants exercised on average 24.5 min (95%CI 18.7–30.5) at moderate intensity (>50–80% VO2peak) and 6.6 min (95%CI 2.2–10.8) at high intensity (>80% VO2peak).

Conclusions
The participants were able to exercise at moderate or high intensity during exergaming in a considerable amount of time. Exergaming seems to be suitable for aerobic exercise at an intensity that can provide health benefits in wheelchair-dependent persons with SCI.
Replicated life-history patterns and subsurface origins of the bacterial sister phyla Nitrospirota and Nitrospinota
Authors: D’Angelo Timothy , Goordial Jacqueline , Lindsay Melody R , McGonigle Julia , Booker Anne , Moser Duane , Stepanauskus Ramunas , Orcutt Beth N 
Published: April 2023

Abstract
The phyla Nitrospirota and Nitrospinota have received significant research attention due to their unique nitrogen metabolisms important to biogeochemical and industrial processes. These phyla are common inhabitants of marine and terrestrial subsurface environments and contain members capable of diverse physiologies in addition to nitrite oxidation and complete ammonia oxidation. Here, we use phylogenomics and gene-based analysis with ancestral state reconstruction and gene-tree–species-tree reconciliation methods to investigate the life histories of these two phyla. We find that basal clades of both phyla primarily inhabit marine and terrestrial subsurface environments. The genomes of basal clades in both phyla appear smaller and more densely coded than the later-branching clades. The extant basal clades of both phyla share many traits inferred to be present in their respective common ancestors, including hydrogen, one-carbon, and sulfur-based metabolisms. Later-branching groups, namely the more frequently studied classes Nitrospiria and Nitrospinia, are both characterized by genome expansions driven by either de novo origination or laterally transferred genes that encode functions expanding their metabolic repertoire. These expansions include gene clusters that perform the unique nitrogen metabolisms that both phyla are most well known for. Our analyses support replicated evolutionary histories of these two bacterial phyla, with modern subsurface environments representing a genomic repository for the coding potential of ancestral metabolic traits.
MiR-122-5p regulates the mevalonate pathway by targeting p53 in non-small cell lung cancer
Authors: Zheng Yu-kun , Zhou Zhong-shi , Wang Guang-zhong , Tu Ji-yuan , Cheng Huan-bo , Ma Shang-zhi , Ke Chang , Wang Yan , Jian Qi-pan , Shu Yu-hang , Wu Xiao-wei 
Published: April 2023

AbstractThe 5-year survival rate of non-small cell lung cancer (NSCLC) patients is very low. MicroRNAs (miRNAs) are involved in the occurrence of NSCLC. miR-122-5p interacts with wild-type p53 (wtp53), and wtp53 affects tumor growth by inhibiting the mevalonate (MVA) pathway. Therefore, this study aimed to evaluate the role of these factors in NSCLC. The role of miR-122-5p and p53 was established in samples from NSCLC patients, and human NSCLC cells A549 using the miR-122-5p inhibitor, miR-122-5p mimic, and si-p53. Our results showed that inhibiting miR-122-5p expression led to the activation of p53. This inhibited the progression of the MVA pathway in the NSCLC cells A549, hindered cell proliferation and migration, and promoted apoptosis. miR-122-5p was negatively correlated with p53 expression in p53 wild-type NSCLC patients. The expression of key genes in the MVA pathway in tumors of p53 wild-type NSCLC patients was not always higher than the corresponding normal tissues. The malignancy of NSCLC was positively correlated with the high expression of the key genes in the MVA pathway. Therefore, miR-122-5p regulated NSCLC by targeting p53, providing potential molecular targets for developing targeted drugs.