bluuebunny/crossref_metadata_2025_split huggingface dataset:
ETV4 Mediated Tumor‐Associated Neutrophil Infiltration Facilitates Lymphangiogenesis and Lymphatic Metastasis of Bladder Cancer
Authors: Zhang Qiang , Liu Sen , Wang Hongjin , Xiao Kanghua , Lu Junlin , Chen Siting , Huang Ming , Xie Ruihui , Lin Tianxin , Chen Xu 
Published: January 2023

AbstractAs a key step of tumor lymphatic metastasis, lymphangiogenesis is regulated by VEGFC‐VEGFR3 signaling pathway mediated by immune cells, mainly macrophages, in the tumor microenvironment. However, little is known whether tumor associated neutrophils are involved in lymphangiogenesis. Here, it is found that TANs infiltration is increased in LN‐metastatic BCa and is associated with poor prognosis. Neutrophil depletion results in significant reduction in popliteal LN metastasis and lymphangiogenesis. Mechanistically, transcription factor ETV4 enhances BCa cells‐derived CXCL1/8 to recruit TANs, leading to the increase of VEGFA and MMP9 from TANs, and then facilitating lymphangiogenesis and LN metastasis of BCa. Moreover, phosphorylation of ETV4 at tyrosine 392 by tyrosine kinase PTK6 increases nuclear translocation of ETV4 and is essential for its function in BCa. Overall, the findings reveal a novel mechanism of how tumor cells regulate TANs‐induced lymphangiogenesis and LN metastasis and identify ETV4 as a therapeutic target of LN metastasis in BCa.
Current State of Cold Atmospheric Plasma and Cancer‐Immunity Cycle: Therapeutic Relevance and Overcoming Clinical Limitations Using Hydrogels
Authors: Živanić Milica , Espona‐Noguera Albert , Lin Abraham , Canal Cristina 
Published: January 2023

AbstractCold atmospheric plasma (CAP) is a partially ionized gas that gains attention as a well‐tolerated cancer treatment that can enhance anti‐tumor immune responses, which are important for durable therapeutic effects. This review offers a comprehensive and critical summary on the current understanding of mechanisms in which CAP can assist anti‐tumor immunity: induction of immunogenic cell death, oxidative post‐translational modifications of the tumor and its microenvironment, epigenetic regulation of aberrant gene expression, and enhancement of immune cell functions. This should provide a rationale for the effective and meaningful clinical implementation of CAP. As discussed here, despite its potential, CAP faces different clinical limitations associated with the current CAP treatment modalities: direct exposure of cancerous cells to plasma, and indirect treatment through injection of plasma‐treated liquids in the tumor. To this end, a novel modality is proposed: plasma‐treated hydrogels (PTHs) that can not only help overcome some of the clinical limitations but also offer a convenient platform for combining CAP with existing drugs to improve therapeutic responses and contribute to the clinical translation of CAP. Finally, by integrating expertise in biomaterials and plasma medicine, practical considerations and prospective for the development of PTHs are offered.
Photoinduced Absorption Spectroscopy of Photoelectrocatalytic Methylene Blue Oxidation on Titania and Hematite: The Thermodynamic and Kinetic Impacts on Reaction Pathways
Authors: Guo Xinyue , Ma Zixuan , Yuan Yuling , Kang Yan , Xu Hong , Mao Zhiping , Ma Yimeng 
Published: January 2023

AbstractPhotoelectrochemical oxidation of methylene blue is investigated, with particular focus on the difference in kinetics and thermodynamics of decoloration and mineralization employing photoinduced absorption spectroscopy. Hematite and titania photoanodes are used for the comparison of both reactions, which is determined to be associated with the depth of the valence band (3.2 vs 2.5 V for titania and hematite, respectively). Methylene blue is mineralized by the titania photoanode, however it is only oxidized to small fragments by hematite. Such difference is related to the valence band potential that provides the thermodynamic driving force for photogenerated holes in both materials. In addition, the kinetic competition of water oxidation is found to occur on titania by controlling the pH of the electrolyte. In the pH 14 electrolyte, mineralization of methylene blue is suppressed due to the faster and dominant kinetics of water oxidation, in contrast to the complete mineralization in the near neutral electrolyte where water oxidation kinetics are modest. These results clearly address the importance considering both thermodynamic and kinetic challenges of methylene blue oxidation, which has been thought to be an easy molecule to oxidize, as the model reaction in the application of photo(electro)catalysis using metal oxides.
Continuous Flow Synthesis of Non‐Symmetrical Ureas from CO2
Authors: Labiche Alexandre , Norlöff Maylis , Feuillastre Sophie , Taran Frederic , Audisio Davide 
Published: January 2023

AbstractWe report on a continuous flow synthetic process of non‐symmetrical ureas, based on the implementation of a Staudinger/aza‐Wittig reaction sequence. This flow technology allows obtaining the key urea functional group directly from the fundamental C1 building block CO2. A library of 26 derivatives, including alkyl/alkyl, alkyl/aryl and aryl/aryl ureas could be synthesised. In addition, the method was also suitable for the synthesis of S‐thiocarbamates. The potential for large‐scale reaction implementation and a first application to continuous flow 13C‐isotope labeling are shown.
Metal‐Organic Frameworks for Photocatalytic Water Splitting and CO2Reduction
Authors: Sun Kang , Qian Yunyang , Jiang Hai‐Long 
Published: January 2023

AbstractPhotocatalytic water splitting and carbon dioxide (CO2) reduction provide promising solutions to global energy and environmental issues. In recent years, metal‐organic frameworks (MOFs), a class of crystalline porous solids featuring well‐defined and tailorable structures as well as high surface areas, have captured great interest toward photocatalytic water splitting and CO2reduction. In this review, the semiconductor‐like behavior of MOFs is first discussed. We then summarize the recent advances in photocatalytic water splitting and CO2reduction over MOF‐based materials and focus on the unique advantage of MOFs for clarifying the structure‐property relationship in photocatalysis. In addition, some representative characterization techniques have been presented to unveil the photocatalytic kinetics and reaction intermediates in MOF‐based systems. Finally, the challenges, and perspectives for future directions are proposed.
Record Ultralarge‐Pores, Low Density Three‐Dimensional Covalent Organic Framework for Controlled Drug Delivery**
Authors: Zhao Yu , Das Saikat , Sekine Taishu , Mabuchi Haruna , Irie Tsukasa , Sakai Jin , Wen Dan , Zhu Weidong , Ben Teng , Negishi Yuichi 
Published: January 2023

AbstractThe unique structural characteristics of three‐dimensional (3D) covalent organic frameworks (COFs) like high surface areas, interconnected pore system and readily accessible active sites render them promising platforms for a wide set of functional applications. Albeit promising, the reticular construction of 3D COFs with large pores is a very demanding task owing to the formation of interpenetrated frameworks. Herein we report the designed synthesis of a 3D non‐interpenetrated stp net COF, namely TUS‐64, with the largest pore size of all 3D COFs (47 Å) and record‐low density (0.106 g cm−3) by reticulating a 6‐connected triptycene‐based linker with a 4‐connected porphyrin‐based linker. Characterized with a highly interconnected mesoporous scaffold and good stability, TUS‐64 shows efficient drug loading and controlled release for five different drugs in simulated body fluid environment, demonstrating the competency of TUS‐64 as drug nanocarriers.
Reversible Single Electron Redox Steps Convert Polycycles with a C3P3 Core to a Planar Triphosphinine
Authors: Coburger Peter , Schweinzer Clara , Li Zhongshu , Grützmacher Hansjörg 
Published: January 2023

AbstractReaction of the imidazolium‐stabilized diphosphete‐diide IDP with trityl phosphaalkyne affords a mixture which contains the molecules 1 a and 1 b with a central C3P3 core, which formally carries a two‐fold negative charge. In order to avoid the formation of an antiaromatic 8π electron system within a conjugated dianionic six‐membered [C3P3]2− ring, 1 a adopts a bicyclic [3.1.0] and 1 b a tricyclic [2.2.0.0] structure which are in a dynamic equilibrium. 1 a, b can be reversibly oxidized to a triphosphinine dication [5]2+ with a central flat aromatic six‐membered C3P3 ring. This two‐electron redox reaction occurs in two single‐electron transfer steps via the 7π‐radical cation [4]⋅+, which could also be isolated and fully characterized. The profound reversible structural change observed for the two‐electron redox couple [5]2+/1 a, b is in sharp contrast to the C6H6/[C6H6]2− couple, which undergoes only a modest structural deformation.
Predicting Highly Enantioselective Catalysts Using Tunable Fragment Descriptors**
Authors: Tsuji Nobuya , Sidorov Pavel , Zhu Chendan , Nagata Yuuya , Gimadiev Timur , Varnek Alexandre , List Benjamin 
Published: January 2023

AbstractCatalyst optimization processes typically rely on inductive and qualitative assumptions of chemists based on screening data. While machine learning models using molecular properties or calculated 3D structures enable quantitative data evaluation, costly quantum chemical calculations are often required. In contrast, readily available binary fingerprint descriptors are time‐ and cost‐efficient, but their predictive performance remains insufficient. Here, we describe a machine learning model based on fragment descriptors, which are fine‐tuned for asymmetric catalysis and represent cyclic or polyaromatic hydrocarbons, enabling robust and efficient virtual screening. Using training data with only moderate selectivities, we designed theoretically and validated experimentally new catalysts showing higher selectivities in a challenging asymmetric tetrahydropyran synthesis.
Designing Water Utility Cooperative Agreements: Lessons Learned in North Carolina
Authors: Gorelick David E. , Gold David F. , Reed Patrick M. , Characklis Gregory W. 
Published: January 2023

Key TakeawaysCooperation among water utilities expands opportunities to provide an affordable, reliable water supply, both now and in the long term.Computational modeling of utility partnerships can explore the potential benefits (and pitfalls) of cooperative infrastructure development.Analysis of agreements for shared treatment plant development in North Carolina provides a framework for design and evaluation of cooperation in other utility contexts.
Oriented‐Etched Graphite for Low‐Temperature Lithium‐Ion Batteries
Authors: Xu Jiang , Wang Xuanding , Hu Bingqing , Ding Jianning , Zhang Zhongqiang , Ge Shanhai 
Published: January 2023

AbstractAs an environmentally friendly energy storage media, lithium‐ion batteries have been extensively used and investigated. However, fast‐charging and low‐temperature tolerance are still huge challenges for the graphite‐based batteries. To alleviate the poor rate performance caused by long diffusion path and the risk of lithium dendrite growth at high rate or low temperature charging, graphite with penetrated macropores is fabricated here, combined with ether‐based electrolyte with low solvation energy, the smooth migration of Li+/Li at the electrolyte‐electrode interface and interior of the graphite is ensured. The product graphite exhibits excellent rate and low‐temperature performance, evidenced by 352.9 mAh g−1 capacity delivered at 2 C‐rate and −30 °C. In addition, benefited from the intact preservation of the pristine graphite structure, it exhibits ∼85 % initial charge‐discharge efficiency and long cycle life (∼94.2 % capacity retention after 500 cycles). This synergic strategy makes the product graphite very efficient in the fast charging/discharging and low temperature occasions.
Sprengabbruch der Salzbachtalbrücken
Authors: Rapps Clarissa , Kina Jens , Fehling Ekkehard , Gündel Max 
Published: January 2023

AbstractWährend des geplanten konventionellen Rückbaus der Salzbachtalbrücken, zweier Autobahnbrücken der BAB A 66 im Wiesbadener Stadtteil Biebrich, versagte am 18. Juni 2021 ein Rollenlager des Südüberbaus. Der Überbau senkte sich auf einen Pfeiler und drückte ihn zur Seite; ferner entstanden Risse im Überbau. Da die Standsicherheit nicht mehr gewährleistet war, wurden die Brücken sowie die darunter verlaufende Mainzer Straße und die Taunus‐Eisenbahn Richtung Wiesbaden Hauptbahnhof gesperrt. Um möglichst schnell die daraus resultierenden erheblichen Verkehrsbehinderungen zu beseitigen, wurde kurzfristig ein Sprengabbruch beider Brücken geplant und durchgeführt. Aufgrund der beengten Platzverhältnisse und schützenswerter Infrastruktur in unmittelbarer Nähe der Abbruchobjekte sah das Konzept vor, zunächst die Südbrücke mittels Vertikalkollaps zu sprengen und anschließend die Nordbrücke mittels Kippkollaps auf die Südbrücke niederzulegen. Die Sprengung der Salzbachtalbrücken erfolgte am 6. November 2021 mit 204 kg Sprengstoff. Alle Pfeiler der Südbrücke knickten planmäßig in Richtung Westen aus, sodass der Überbau exakt senkrecht abstürzte. Die Nordbrücke folgte 3 Sekunden später und kippte planmäßig nach Süden. In diesem Beitrag werden die Historie des ursprünglich geplanten konventionellen Rückbaus, die Havarie sowie die Sprengabbruchplanung und ‐durchführung der Brücken erörtert.
Distalization using efficient attachment protocol in clear aligner therapy—A case report
Authors: Sabouni Waddah , Muthuswamy Pandian Srirengalakshmi , Vaid Nikhilesh R. , Adel Samar M. 
Published: January 2023

AbstractTo describe an esthetic orthodontic treatment using aligners in an adult patient with dental class II malocclusion associated with crowding. A 25‐year‐old female patient with skeletal class I, bilateral class II relation, increased overjet and overbite and crowding in both arches presented for orthodontic treatment. The patient refused conventional fixed multibracket treatment in favor of aligners. Pre‐ and post‐treatment records are presented. Treatment objectives were achieved in 10 months, and the patient was satisfied with the functional and esthetic outcomes. Combining aligners with appropriate attachment location and geometry is an efficacious means of resolving orthodontic issues such as class II malocclusion in a time frame comparable to that of conventional fixed orthodontics. Staging in distalization increases the predictability of movement. Furthermore, this system is associated with optimal oral hygiene and excellent esthetics.
Intramolecular 1,2 C−H Addition of o‐Methyl Groups to Form Unique Ruthenium Pincer Tuck‐in Complexes
Authors: Culver Damien B. , Boncella James M. 
Published: January 2023

AbstractNew RPNHP ligands containing 2,4‐xylyl (4mXPNHP) and mesityl (MesPNHP) groups on the phosphorus atoms were synthesized. 4mXPNHP reacts with [(cymene)RuCl2]2 followed by PMe3 to produce κ3‐4mXPNHPRu(PMe3)Cl2. MesPNHP reacts with [(cymene)RuCl2]2 to produce monomeric κ3‐MesPNHPRuCl2 that reacts with CO forming κ3‐MesPNHPRu(CO)Cl2. Surprisingly, dehydrohalogenation of these complexes results in the activation of ortho methyl groups of the pincer ligands, rather than formation of κ3‐RPNPRuLCl complexes. This results from transient κ3‐RPNPRuLCl formation followed by 1,2‐addition of an ortho C−H bond across the Ru‐amide bond. The transient amide complex of κ4‐4mXPNHPRu(PMe3)Cl was trapped with CO forming κ3‐4mXPNPRu(PMe3)(CO)Cl. In contrast, κ4‐MesPNHPRu(CO)Cl does not react with ligands to trap the expected amide complex of reverse C−H addition. Instead, CO and PMe3 displace the chloride ligand forming cationic complexes. In both cases, hemi‐lability of the pincer ligand was observed spectroscopically. The new complexes serve as precursors to moderately active catalysts for the acceptorless dehydrogenative coupling of n‐butanol.
Influence of the Distance between Two Catalysts for CO2 to Dimethyl Ether Tandem Reaction
Authors: Javed Mudassar , Brösigke Georg , Schomäcker Reinhard , Repke Jens-Uwe 
Published: January 2023

AbstractTandem reactions, with their great potential for reduction of capital and operational costs, reaction time, and byproducts are becoming more prominent. Among possible tandem reaction systems for direct conversion of CO2 to green fuel, dimethyl ether synthesis via methanol route is of vital importance. However, there are several parameters which must be considered for the combination of two catalysts to achieve promising performance, of those, the distance between the two catalysts is a crucial one. This distance can affect the overall performance of the tandem system by causing external mass transfer limitations or the poisoning of the catalysts. This work presents a systematic study on the influence of the distance between the two catalysts for the CO2 to dimethyl ether tandem system.
Electrochemical Synthesis and Analytical Characterization of Hybrid Zinc/Calcium Antimicrobial Nano‐Oxides for Cultural Heritage Applications
Authors: Izzi Margherita , Chiara Sportelli Maria , Anna Picca Rosaria , Cioffi Nicola 
Published: January 2023

AbstractThe present study proposes the one‐step synthesis of Zn/Ca hybrid nano‐oxides combining an electrochemical route to ZnO nanostructures (NSs) with the wet chemical production of Ca(OH)2 NSs. ZnO is a known antimicrobial and Ca(OH)2 has consolidating properties, both appealing for stone artwork preservation in the field of cultural heritage. Two concentrations of the electrolytic bath (NaOH 0.1 and 0.4 M) were studied resulting in distinct final nanomaterials (Zn−Ca0.1 and Zn−Ca0.4). Fourier transform infrared, X‐ray photoelectron spectroscopies, and transmission electron microscopy demonstrated that Zn−Ca0.1 consisted of lamellar calcium hydroxyzincate NSs, whereas Zn−Ca0.4 showed hexagonal Ca(OH)2 (∼500 nm) and ZnO (∼50 nm) NSs. Nanocoatings prepared with Zn−Ca0.1 exhibited a Zn2+ release increasing up to 1 μmol cm−2 in 24 h. Instead, Zn−Ca0.4‐based nanocoatings released a constant value of about 0.35 μmol/cm2. Both types of nanocoatings showed good antimicrobial activity against Bacillus subtilis after 48 h and hydrophilic behavior over 28 days.
Photoactive Organo‐Sulfur Polymers for Hydrogen Generation
Authors: Tufts Noah Q. , Chiu Nan Chieh , Musa Emmanuel Nyela , Gallagher Trenton C. , Fast Dylan B. , Stylianou Kyriakos C. 
Published: January 2023

AbstractHerein, we report the synthesis of photoactive polymeric organo‐sulfur (POS) materials. These polymers absorb light in the ultraviolet/visible and near‐infrared region of the solar spectrum, and upon irradiation, they reduce water to hydrogen (H2). The decoration of POS materials with nitrile (−CN) groups is found to be the critical factor for enhanced interactions with the co‐catalyst, Ni2P, leading to greater H2 evolution rates compared to the nitrile‐free POS material.
The Biologically Inspired Abilities of Metallosupramolecular Architectures
Authors: Pearcy Aston C. , Crowley James D. 
Published: January 2023

AbstractNatural machinery such as proteins and enzymes can bind substrates and perform intricate functions on these molecules. This behaviour is mediated by highly ordered but conformationally flexible structures dictated through favourable intra‐ and intermolecular interactions. Metallosupramolecular architectures (MSAs) function as synthetic machinery that are responsive to their environment, and display similar, but less impressive, abilities to their biological counterparts. Natural and synthetic systems share the properties of molecular recognition and catalysis facilitated through the often complex structures of these architectures. This article outlines efforts to use metallosupramolecular structures to mimic the properties of biological enzymes and machines using important recent examples from the field.
The Mechanochemical Fries Rearrangement: Manipulating Isomer Ratios in the Synthesis of p‐Hydroxyacetophenone at Different Scales**
Authors: Baier Daniel M. , Rensch Tilo , Bergheim Konrad , Pietryga Viktoria , Grätz Sven , Borchardt Lars 
Published: January 2023

AbstractHerein, the first mechanochemical Fries rearrangement for the industrially important synthesis of para‐hydroxyacetophenone, inside a ball mill and a twin‐screw extruder, is presented. Our approach leads to a yield of 62 % in as little as 90 minutes while liquid‐assisted grinding can shift the isomer ratio resulting in an excess of the desired para‐product. The multigram scale‐up by extrusion leads to 61 % yield in only three minutes while completely avoiding solvents. The extrusion temperature can even further be reduced by combining extrusion with a subsequent ageing step.
Zinc Substituted Myoglobin−Albumin Fusion Protein: A Photosensitizer for Cancer Therapy
Authors: Yamada Taiga , Morita Yoshitsugu , Takada Ryoya , Funamoto Mizuki , Okamoto Wataru , Kohno Mitsutomo , Komatsu Teruyuki 
Published: January 2023

AbstractMyoglobin combined with human serum albumin (Mb‐HSA) can be produced using yeast Pichia pastoris as a host strain, with secretion into the culture medium. This Mb‐HSA fusion protein possesses identical O2 binding affinity to that of naked Mb. The Mb unit is reconstituted with a zinc(II) protoporphyrin IX, yielding (zinc substituted Mb)‐HSA, ZnMb‐HSA. The photophysical property and singlet O2 generation ability of ZnMb‐HSA are equivalent to those of ZnMb. In vitro cell experiments revealed that ZnMb‐HSA acts as a superior photosensitizer for photodynamic cancer therapy. It is noteworthy that ZnMb‐HSA shows long circulation lifetime in vivo.
Glia Cell Morphology Analysis Using the Fiji GliaMorph Toolkit
Authors: Kugler Elisabeth , Breitenbach Eva‐Maria , MacDonald Ryan 
Published: January 2023

AbstractGlial cells are the support cells of the nervous system. Glial cells typically have elaborate morphologies that facilitate close contacts with neighboring neurons, synapses, and the vasculature. In the retina, Müller glia (MG) are the principal glial cell type that supports neuronal function by providing a myriad of supportive functions via intricate cell morphologies and precise contacts. Thus, complex glial morphology is critical for glial function, but remains challenging to resolve at a sub‐cellular level or reproducibly quantify in complex tissues. To address this issue, we developed GliaMorph as a Fiji‐based macro toolkit that allows 3D glial cell morphology analysis in the developing and mature retina. As GliaMorph is implemented in a modular fashion, here we present guides to (a) setup of GliaMorph, (b) data understanding in 3D, including z‐axis intensity decay and signal‐to‐noise ratio, (c) pre‐processing data to enhance image quality, (d) performing and examining image segmentation, and (e) 3D quantification of MG features, including apicobasal texture analysis. To allow easier application, GliaMorph tools are supported with graphical user interfaces where appropriate, and example data are publicly available to facilitate adoption. Further, GliaMorph can be modified to meet users’ morphological analysis needs for other glial or neuronal shapes. Finally, this article provides users with an in‐depth understanding of data requirements and the workflow of GliaMorph. © 2023 The Authors. Current Protocols published by Wiley Periodicals LLC.Basic Protocol 1: Download and installation of GliaMorph components including example dataBasic Protocol 2: Understanding data properties and quality 3D—essential for subsequent analysis and capturing data property issues earlyBasic Protocol 3: Pre‐processing AiryScan microscopy data for analysisAlternate Protocol: Pre‐processing confocal microscopy data for analysisBasic Protocol 4: Segmentation of glial cellsBasic Protocol 5: 3D quantification of glial cell morphology