bluuebunny/crossref_metadata_2025_split huggingface dataset:
Kaempferol suppresses glioma progression and synergistically enhances the antitumor activity of gefitinib by inhibiting the EGFR/SRC/STAT3 signaling pathway
Authors: Zhou Jiabin , Liu Yuhan , Chen Jun , Xiong Nanxiang , Yi Dongye 
Published: March 2023

AbstractKaempferol (Kae) is a natural flavonoid that has multiple biological activities, such as anti‐inflammatory and antitumor activities. However, few studies have been reported on antiglioma effects of Kae. This study aimed to explore the effects and potential mechanisms of Kae and synergistic antitumor activities with gefitinib (Gef) on glioma. Cell Counting Kit‐8 and 5‐ethynyl‐2'‐deoxyuridine assays were used to detect cytotoxicity and cell proliferation. Cell apoptosis and the cell cycle were detected by flow cytometry. Transwell assays were used to detect the migratory and invasive abilities of glioma cells. Network pharmacology and molecular docking analysis were used to screen for core targets of Kae in glioma therapy. Xenograft tumor nude mice were established with U251 cells to verify the antiglioma effects of Kae in vivo. A terminal deoxynucleotidyl transferase dUTP nick end labeling assay was used to detect apoptosis in tumor tissues. The expression of proteins was detected by immunohistochemistry and western blot analysis. Kae inhibited cell proliferation, promoted apoptosis, and induced cell cycle arrest in the G2/M phase of glioma cells in a concentration‐dependent manner. Kae inhibited the migration and invasion of glioma cells at low concentrations. Network pharmacology analyses showed that epidermal growth factor receptor (EGFR) and SRC proto‐oncogene (SRC) might be direct molecular‐binding targets of Kae. Our results showed that Kae inhibited the levels of phosphorylated EGFR, phosphorylated SRC (p‐SRC), and phosphorylated signal transducer and activator of transcription 3 (STAT3). In addition, the combination of Kae with Gef significantly inhibited the proliferation of glioma cells. Kae further inhibited EGFR phosphorylation after treatment with Gef. Similarly, Kae further enhanced the inhibition of p‐SRC caused by SU6656. Finally, we demonstrated that Kae exerted great antitumor activities and enhanced the antitumor effect of Gef by inhibiting EGFR/SRC/STAT3 signaling pathway in vivo. Kae played a potential role and synergistic antiglioma effects with Gef by inhibiting the phosphorylation of EGFR/SRC dual targets. Kae is expected to be a candidate drug or chemosensitizer in glioma therapy.
Extreme drought disrupts plant phenology: Insights from 35 years of cloud forest data in Venezuela
Authors: Flores Saúl , Forister Matthew L. , Sulbaran Hendrik , Díaz Rodrigo , Dyer Lee A. 
Published: March 2023

AbstractThe potential effects of climate change on plant reproductive phenology include asynchronies with pollinators and reductions in plant fitness, leading to extinction and loss of ecosystem function. In particular, plant phenology is sensitive to extreme weather events, which are occurring with increasing severity and frequency in recent decades and are linked to anthropogenic climate change and shifts in atmospheric circulation. For 15 plant species in a Venezuelan cloud forest, we documented dramatic changes in monthly flower and fruit community composition over a 35‐year time series, from 1983 to 2017, and these changes were linked directly to higher temperatures, lower precipitation, and decreased soil water availability. The patterns documented here do not mirror trends in temperate zones but corroborate results from the Asian tropics. More intense droughts are predicted to occur in the region, which will cause dramatic changes in flower and fruit availability.
Inferring functional diversity from environmental DNA metabarcoding
Authors: Condachou Céline , Milhau Tristan , Murienne Jérôme , Brosse Sébastien , Villéger Sébastien , Valentini Alice , Dejean Tony , Mouillot David 
Published: March 2023

AbstractEnvironmental DNA (eDNA) metabarcoding is a reliable method to assess taxonomic diversity, but the incompleteness of genetic reference databases prevents the assignation of many sequences to a given species. Functional diversity (FD) is a key biodiversity facet to monitor, but it requires the identification of all species within communities to account for their trait values. So, the ability of eDNA‐based inventories to estimate the “true” level of FD is unknown. Here, using fish surveys in two representative temperate and tropical rivers, with a quasi‐exhaustive genetic and trait database, we measured the bias and variability of different FD indices when calculated with uncertainty in taxonomic assignment at the genus and family level. Our results show that when measuring FD indices with species randomly chosen within genera and families, the bias cannot exceed 30% from real observed FD values. The variability is higher for species‐poorer communities and when those communities are composed of genera and families with high functional heterogeneity. Despite taxonomic uncertainty, our results demonstrate the potential of eDNA surveys to estimate reliable FD values.
Prediction on onset conditions of alpha, beta, and gamma type free piston Stirling generators
Authors: Chi Chunyun , Mou Jian , Lin Mingqiang , Li Ruijie , Jiao Kexin , Hong Guotong 
Published: March 2023

AbstractThe study of the onset characteristics of the free piston Stirling generator (FPSG) is essential for its development. In this study, the onset characteristics of the FPSG were discussed based on a thermodynamic model using the Routh stability criterion. First, the onset conditions of three types of FPSG, alpha, beta, and gamma, were analyzed. Then an experimental and numerical study of a 100 W beta‐type FPSG prototype was conducted. The impacts on the onset temperatures of spring stiffness, charging pressure, and external load were further explored. The results demonstrate that no matter how the parameters are altered, alpha‐type FPSG cannot start up. Whether beta and gamma type FPSG can start up depends on the design and operating parameters. For the prototype, as the displacer spring stiffness increases, the onset temperature rises first and then drops. When the displacer spring stiffness is 19.2 N/mm, the corresponding onset temperature is the lowest of 489 K. The FPSG is easier to start up at a higher external load and higher charging pressure. The experimental results of the impacts of the spring stiffness, charging pressure, and external load on the onset temperature agree well with the calculated results, with errors ranging from 1.7% to 9.12%. The proposed approach can play a guiding significance in studying FPSG onset conditions.
Optimization of battery energy storage system size and power allocation strategy for fuel cell ship
Authors: Cao Wei , Geng Pan , Xu Xiaoyan 
Published: March 2023

AbstractThe fuel cell system (FCS) is commonly combined with an energy storage system (ESS) for enhancing the performance of the ship. Consequently, the battery ESS size and power allocation strategy are critical for the hybrid energy system. This paper focuses on designing a method to solve these two problems. First, a battery degradation model is employed to assess the ESS lifetime. Subsequently, the sizing problem and the optimal power allocation are integrated into a cost‐minimization problem, which is solved by a double‐loop optimization approach. The inside loop utilizes the battery degradation model to calculate ESS lifetime. In the outside loop, a power allocation strategy based on the hybrid Particle Swarm Optimization algorithm and Gray Wolf Optimization algorithm is presented. Finally, the power allocation strategy is extended to real‐time implementation by the equivalent consumption minimization strategy (ECMS) and an improved ECMS is proposed to make the FCS operates near the maximal efficiency point. Compared with ECMS, the operating cost reduces by 0.26%. The result indicates that the proposed method can optimize the ESS size efficiently, and the power allocation strategy can assure the stable operation of the fuel cell ship.
Bone tissue engineering for treating osteonecrosis of the femoral head
Authors: Bian Yixin , Hu Tingting , Lv Zehui , Xu Yiming , Wang Yingjie , Wang Han , Zhu Wei , Feng Bin , Liang Ruizheng , Tan Chaoliang , Weng Xisheng 
Published: March 2023

AbstractOsteonecrosis of the femoral head (ONFH) is a devastating and complicated disease with an unclear etiology. Femoral head‐preserving surgeries have been devoted to delaying and hindering the collapse of the femoral head since their introduction in the last century. However, the isolated femoral head‐preserving surgeries cannot prevent the natural progression of ONFH, and the combination of autogenous or allogeneic bone grafting often leads to many undesired complications. To tackle this dilemma, bone tissue engineering has been widely developed to compensate for the deficiencies of these surgeries. During the last decades, great progress has been made in ingenious bone tissue engineering for ONFH treatment. Herein, we comprehensively summarize the state‐of‐the‐art progress made in bone tissue engineering for ONFH treatment. The definition, classification, etiology, diagnosis, and current treatments of ONFH are first described. Then, the recent progress in the development of various bone‐repairing biomaterials, including bioceramics, natural polymers, synthetic polymers, and metals, for treating ONFH is presented. Thereafter, regenerative therapies for ONFH treatment are also discussed. Finally, we give some personal insights on the current challenges of these therapeutic strategies in the clinic and the future development of bone tissue engineering for ONFH treatment.
An integrated pasture biomass and beef cattle liveweight predictive model under weather forecast uncertainty: An application to Northern Australia
Authors: Masoud Mahmoud , Hsieh Jeff , Helmstedt Kate , McGree James , Corry Paul 
Published: March 2023

AbstractBeef production drives a significant proportion of Australia's agricultural economy, representing AUD11 billion in agricultural production value annually. The profitability of cattle farms across seasons varies greatly and is particularly influenced by weather and associated uncertainty through pasture growth. Furthermore, producers must be careful in managing stocking rates to avoid overgrazing which has a significant environmental and economic impact. Predictive modelling of pasture and cattle liveweight can help producers to better manage this difficult balancing act. In this paper, we integrate a model for pasture growth and cattle weight gain to formulate predictions of liveweight production volume and pasture biomass. Monte‐Carlo simulation was used to understand the impact of uncertainty in model inputs relating to weather. Through such an approach, a distribution of possible pasture outcomes and beef production is formed which can be inspected to determine, for example expected production volume and risk of overgrazing. The methodology is demonstrated and validated through application to case study data sourced from Australian beef producers. Results demonstrate that it is possible to obtain reasonable predictions of liveweight gain and highlight the importance of considering the associated uncertainty. The accuracy of the proposed models has been examined by calculating statistical parameters MSE, MAE and R2. This approach can provide insight into the distribution of possible production outcomes given the long‐term rainfall outlook. The use of such predictive models and simulations by producers will lead to better‐informed planning of stocking rates, with benefits to productivity and sustainable utilisation of pasture.
The volatile flavor and the antioxidant properties of a novel chrysanthemum rice wine during natural aging
Authors: Zhang Yu , Yin Huai‐Ning , Zhao Ting , Zhan Cheng , Wang Ai‐Yuan , Xu Yang‐Hui , Chen Mao‐Bin 
Published: March 2023

AbstractChrysanthemum morifolium cv. Fubaiju, a traditional tea in southern China with high nutritional and health functions was used in this study. Optimized production conditions of a novel chrysanthemum rice wine (FRW) were obtained by the Box–Behnken design response surface experiment. FRW with best sensory quality was developed with 0.68% chrysanthemum, 0.79% Jiuqu and 0.81:1 liquid‐to‐solid ratio. Compared with rice wine (RW) control, the total phenolic and flavonoid contents, as well as antioxidant activity of the FRW increased significantly. GC–MS analysis showed that more flavor compounds including alcohols, aldehydes, acids, and esters were detected in FRW. During the aging process, it was found that the antioxidant substances, the antioxidant activity and the flavor substances decreased, with the wine body tending to be homogenized. After 6 months of storage, overall sensory quality of FRW was more harmonious, with special nectar taste, which dramatically improved the flavor characteristics and functionality compared with traditional RW.
Preparation, characterization, identification, and antioxidant properties of fermented acaí (Euterpe oleracea)
Authors: Liu Wen‐Ying , Wang Xue , Ren Jie , Zheng Cheng‐Dong , Wu Han‐Shuo , Meng Fan‐Tong , Ling Kong , Qi Xiu‐Yu , Zhou Ming , Wang Yue , Gu Rui‐Zeng , Han Lu‐Jia , Zhang Yong‐Jiu 
Published: March 2023

AbstractFermentation technology was used to prepare the acaí (Euterpe oleracea) fermentation liquid. The optimal fermentation parameters included a strain ratio of Lactobacillus paracasei: Leuconostoc mesenteroides: Lactobacillus plantarum = 0.5:1:1.5, a fermentation time of 6 days, and a nitrogen source supplemental level of 2.5%. In optimal conditions, the ORAC value of the fermentation liquid reached the highest value of 273.28 ± 6.55 μmol/L Trolox, which was 55.85% higher than the raw liquid. In addition, the FRAP value of the acaí, as well as its scavenging ability of DPPH, hydroxyl, and ABTS free radicals, increased after fermentation. Furthermore, after fermentation treatment, the microstructure, basic physicochemical composition, amino acid composition, γ‐aminobutyric acid, a variety of volatile components, and so on have changed. Therefore, fermentation treatment can significantly improve the nutritional value and flavor of the acaí. This provides a theoretical basis for the comprehensive utilization of acaí.
Neuroprotective effect of quercetin and Zingiber officinale on sodium arsenate‐induced neurotoxicity in rats
Authors: Yousuf Rasia , Verma Pawan Kumar , Sharma Priyanka , Sood Shilpa , Bhatti Muhammad A. , Bhat Zuhaib F. 
Published: March 2023

AbstractThe study was aimed at determining the ameliorative potential of quercetin and Zingiber officinale (ZO) against sodium arsenate‐induced neurotoxicity in male Wistar rats. Thirty adult animals were randomly allocated to five groups (n = 6). Group I served as control, groups II and IV were treated with ZO [300 mg/kg, PO (per os)/day], and group V animals were administered quercetin (50 mg/kg, PO/day) for 18 days. Groups III, IV, and V were injected with sodium arsenate (20 mg/kg, intraperitoneally/day) for 4 days starting from day 15. The administration of sodium arsenate resulted in a significant decrease in total antioxidant status, total thiols, superoxide dismutase, catalase, glutathione peroxidase, glutathione reductase, and aryl esterase in brain tissue of the animals compared with the control group. In addition, a significant increase was observed in malondialdehyde, advanced oxidation protein product and plasma nitric oxide levels, indicating oxidative stress‐mediated neuronal damage. However, these arsenic‐induced alterations were significantly reversed by quercetin or ZO in the treatment groups, indicating their ameliorative potential. These positive effects were further confirmed by histopathological examination of brain tissue revealing the suppression of severe neuronal injury, spongiosis and gliosis in the samples pretreated with quercetin and ZO. Our results suggest that inclusion of ZO and quercetin‐rich foods in the diet can help in preventing the neurotoxic effects in areas with elevated levels of arsenic in food chain and ground water.
Development of lentil peptides with potent antioxidant, antihypertensive, and antidiabetic activities along with umami taste
Authors: Rezvankhah Amir , Yarmand Mohammad Saeid , Ghanbarzadeh Babak , Mirzaee Homaira 
Published: March 2023

AbstractLentil peptides have shown promising bioactive properties regarding the antioxidant activity and also inhibitory activity of angiotensin‐I‐converting enzyme (ACE). Sequential hydrolysis of proteins has shown a higher degree of hydrolysis with enhanced antioxidant and ACE‐inhibitory activities. The lentil protein concentrate (LPC) was sequentially hydrolyzed using Alcalase and Flavourzyme at 2% w/w. The hydrolysate (LPH) was cross‐linked (LPHC) or sonicated (LPHUS) and sequentially cross‐linked (LPHUSC). Amino acid profile, molecular weight (MW) distribution, DPPH and ABTS radical scavenging activities (RSA; 7 mg/mL), ACE (0.1–2 mg/mL), α‐glucosidase, and α‐amylase inhibitory activities (10–500 μg/mL), and umami taste were determined. The highest DPPH RSA was obtained for LPH (68.75%), followed by LPHUSC (67.60%), and LPHUS (67.49%) while the highest ABTS RSA values were obtained for LPHC (97.28%) and LPHUSC (97.20%). Cross‐linking and sonication led to the improvement of the ACE‐inhibitory activity so that LPHUSC and LPHC had IC50 values of 0.23 and 0.27 mg/mL, respectively. LPHC and LPHUSC also indicated higher α‐glucosidase inhibitory activity (IC50 of 1.2 and 1.23 mg/mL) compared to LPH (IC50 of 1.74 mg/mL) and LPHUS (IC50 of 1.75 mg/mL) while the IC50 value of acarbose indicated 0.51 mg/mL. Moreover, LPHC and LPHUSC exhibited higher α‐amylase inhibitory activities (IC50 of 1.35 and 1.16 mg/mL) than LPHUS (IC50 of 1.95 mg/mL), and LPH (IC50 of 2.51 mg/mL) while acarbose had an IC50 value of 0.43 mg/mL. Umami taste analysis revealed that LPH and LPHC due to MW of 1.7 and 2.3 kDa and also high umami amino acids could be well considered as representative of meaty and umami analog flavors while indicating stronger antioxidant, antihypertension, and antidiabetic attributes.
Real‐world data for precision cancer medicine—A European perspective
Authors: Christopoulos Petros , Schlenk Richard , Kazdal Daniel , Blasi Miriam , Lennerz Jochen , Shah Rajiv , Budczies Jan , Malek Nisar , Fröhling Stefan , Rosenquist Richard , Schirmacher Peter , Bozorgmehr Farastuk , Kuon Jonas , Reck Martin , Thomas Michael , Stenzinger Albrecht 
Published: March 2023

AbstractLeveraging real‐world data (RWD) for drug access is necessary to overcome a key challenge of modern precision oncology: tackling numerous low‐prevalence oncogenic mutations across cancers. Withholding a potentially active medication in patients with rare mutations for the sake of control chemotherapy or “best” supportive care is neither practicable nor ethically justifiable anymore, particularly as RWD could meanwhile be used instead, according to scientific principles outlined by the US Food and Drug Administration, European Medicines Agency and other stakeholders. However, practical implementation varies, with occasionally opposite recommendations based on the same evidence in different countries. In the face of growing need for precision drugs, more transparency of evaluation, a priori availability of guidance for the academia and industry, as well as a harmonized framework for health technology assessment across the European Union (EU) are imperative. These could in turn trigger infrastructural changes in national and pan‐European registries, cancer management guidelines (e.g., frequency of routine radiologic restaging, inclusion of patient‐reported outcomes), and the health data space, to ensure conformity with declared standards and facilitate extraction of RWD sets (including patient‐level data) suitable for approval and pricing with minimal effort. For an EU‐wide unification of precision cancer medicine, collective negotiation of drug supply contracts and funding solidarity would additionally be required to handle the financial burden. According to experience from pivotal European programs, off‐label use could potentially also be harmonized across EU‐states to accelerate availability of novel drugs, streamline collection of valuable RWD, and mitigate related costs through wider partnerships with pharmaceutical companies.
The petrogenesis and mineralization of Zhaojinggou Nb–Ta deposit, Inner Mongolia: Evidence from geochronology, rock, and mineral geochemistry
Authors: Li Xue , Wang Keyong , Sun Guosheng , Zhang Jitian , Liu Genyi , Ma Dong , Sun Jiuda , He Xin , Wang Guangwei 
Published: March 2023

The Zhaojinggou Nb–Ta deposit is one of large rare metal deposits newly discovered in the northern margin of the North China Craton in recent years. This paper reports petrography, petrochemistry, columbite‐group minerals U–Pb chronology study of the amazonite granitic pegmatite (AGP) exposed in this deposit, and composition of columbite‐group minerals and biotite are obtained by electron probe microanalyzer and LA‐ICP‐MS. Eighteen analyses of columbite‐group minerals yielded weighted mean 206Pb/238U age of 116.9 ± 1.4 Ma. The crystallization temperature of biotite is 630–650°C, and the oxygen fugacity is 10−17–10−18 bars. The biotite has low MgO contents and high Rb, Rb/Sr, and FeOT/(FeOT + MgO) ratios. The AGP has extremely low MgO, Cr, Co and Ni contents, with Nb/Ta ratios range from 1.63 to 9.05 and Rb/Sr ratios range from 303.30 to 648.90, and obvious ‘M’ type tetrad effect of rare earth element indicating that the formation of the AGP is related to crust‐derived magma. The contents of Nb2O5 and FeO decrease, while the Ta2O5 and WO3 contents, Mn# and Ta# values increase gradually from the core to the rim of columbite‐group minerals. Some columbite‐group minerals have unobvious oscillatory zoning, and some have a clear bright zoing with high Ta contents on the rim, indicating that the genesis of Zhaojinggou Nb–Ta deposit is mainly magmatic crystallization differentiation, accompanied by hydrothermal autometasomatism in the late stage.