bluuebunny/crossref_metadata_2025_split huggingface dataset:
Influence of yeast inoculum (Saccharomyces cerevisiae and Torulaspora delbrueckii) on the production of rosé wines from high hydrostatic pressure-treated musts
Authors: Villar Natalia , Pérez-Nevado Francisco , Andrés Ana I. , García-Parra Jesús , Ramírez Manuel , Valdés M. Esperanza , Moreno Daniel 
Published: January 2025

AbstractAutochthonous and mixed cultures of selected yeasts (Saccharomyces and non-Saccharomyces) are used to produce wines with unique physical, chemical, and sensory characteristics. High hydrostatic pressure (HHP) is being used in fermented beverages industries to eliminate undesired microorganisms. The aims of this study were (a) to determine the effect of HHP on a multivarietal must, (b) to analyze the effect of different yeast starter inocula (Saccharomyces cerevisiae, Torulaspora delbrueckii and a mixed culture of both yeasts) on the kinetics fermentation and the characteristics of rosé wines obtained. The HHP treatment inactivated the grape wild yeast population, modifiying the total phenolic content, the anthocyanin profile and the chromatic characteristics of treated must. Similar kinetics were observed in fermentations with S. cerevisiae (WSC), and Mix starters (WMIX); however, T. delbrueckii fermentations (WTD) were sluggish. WTD wines showed lower alcohol strength and tanins values, higher dry extract and residual sugar than WSC and WMIX wines. The following trend was observed in the values of different anthocyanin/non anthocyanin ratios WTD > WMIX > WSC; these could influence the color evolution of the wines elaborated. All wines showed similar and typical chromatic values for rosé wines; and not visual differences were perceived by the tasters. WTD showed a different taste with lower scores in acidity, bitterness and astringency. WTD could be considered a very trendy type of beverage: a sweeter, low-alcohol rosé wine, although WMIX had the highest aromatic complexity.
Preoperative Oral Feeding in Infants with Congenital Heart Disease Within the First Month of Life is Associated with a Higher Likelihood of Freedom From Tube Feeding at Time of Postoperative Discharge
Authors: Dabbagh Aseel , Miller Sarah , McCulloch Michael , Rosenthal Geoffrey , Conaway Mark , White Shelby 
Published: January 2025

AbstractThough several studies have demonstrated that preoperative oral feeding (PO) can be safe in patients with congenital heart disease, they are commonly prohibited from doing so, potentially precluding the development of such skills. We sought to determine whether preoperative oral feeding is associated with freedom from tube feeding at postoperative discharge. Single-center, observational study including patients in the first month of life (≤ 30 days of age) who underwent a single cardiac surgery between 7/1/2017–6/30/2022 and survived to discharge. Preoperative PO was defined as any oral intake up to the day of cardiac surgery. General demographics and peri-operative characteristics were analyzed. A total of 235 patients were included of which 178 (78%) PO fed preoperatively, and 171 (73%) were discharged taking full PO. Those discharged without tube feeds received more preoperative oral feeds, were less likely to have a chromosomal abnormality/genetic syndrome, had lower STAT categories, and were less likely to have postoperative complications, vocal cord dysfunction or prolonged mechanical ventilation. Multivariate analysis found that any preoperative PO [odds ratio (OR) of 2.78 (CI 1.48, 5.24, p-value = 0.002)] and increasing amounts of PO were predictive of full PO intake at discharge [≤ 20 ml/kg/day (OR 2.06, CI 1.03, 4.14, p-value = 0.042) and > 20 ml/kg/day (OR 4.07, CI 1.88, 8.84, p-value = 0.004)]. Preoperative oral feeding is a strong predictor of discharging with full PO intake after cardiac surgery in the first month of life and that it may also improve with increasing volumes. Multi-institutional analyses are warranted.
Evaluation of MALDI-TOF for identification of Vibrio cholerae and Vibrio parahaemolyticus from growth on agar media
Authors: Banerjee Swapan , Flint Annika , Brosseau Madeleine B. , Weedmark Kelly , Shutinoski Bojan 
Published: January 2025

Abstract
Two methods were compared for their ability to accurately identify Vibrio species of interest: whole genome sequencing as the reference method and MALDI-TOF MS (matrix-assisted laser desorption/ionization-time of flight mass spectrometry) proteome fingerprinting. The accuracy of mass spectrometry–based identification method was evaluated for its ability to accurately identify isolates of Vibrio cholerae and Vibrio parahaemolyticus. Identification result of each isolate obtained by mass spectrometry was compared to identification by whole genome sequencing (WGS). The MALDI-TOF MS system had excellent performance for identification of V. cholerae and V. parahaemolyticus isolates grown on a non-selective solid agar media. Unlike the biochemical characterization performed by API20E. In this study, 161 isolates (V. cholerae, n = 33; V. parahaemolyticus, n = 102; V. spp., n = 23; other enteropathogens, Salmonella and E. coli, n = 3) were used to assess accuracy. The MALDI-TOF MS system was able to accurately identify 100% (33/33) of the V. cholerae isolates and 99.9% (101/102) of V. parahaemolyticus isolates, with 100% for both sensitivity and specificity for V. cholerae and 99% sensitivity and 98% specificity for V. parahaemolyticus. Thus, mass spectrometry for bacterial identification is comparable to the WGS. Furthermore, in comparison to a biochemical characterization, the use of MALDI-TOF MS system shortens the analysis time from over 72 h to less than 24 h.

Key points
• V. cholerae and V. parahaemolyticus were successfully ID-ed by MALDI-TOF
• MALDI-TOF sensitivity and specificity parallels the WGS method of identification
• MALDI-TOF is several days faster than the battery of culture-dependent methods
Decoding Periorbital Aging: A Multilayered Analysis of Anatomical Changes
Authors: Lohakitsatian Pongsak , Tunlayadechanont Padcha , Tantitham Thiti 
Published: January 2025

Abstract

Background
Periorbital aging is a complex phenomenon that involves multiple layers of facial anatomy, including bone, fat, and globe. While previous studies have predominantly focused on age-related changes in facial fat compartments, this research aims to provide a comprehensive understanding of all periorbital components, including upper and lower orbital fat, orbital cavity volume, globe volume, and globe position, in the context of aging.

Methods
We conducted a retrospective study involving 118 patients (236 subjects) aged 18–99 years who underwent brain MRI using a 3 Tesla MR system. Baseline demographics and various parameters pertaining to periorbital aging were collected, and comprehensive measurements were obtained through meticulous radiological analysis.

Results
Our findings revealed distinct patterns of age-related changes in the periorbital region. Upper orbital fat remained stable with age, while lower orbital fat exhibited a substantial increase in both anterior and posterior compartments. Notably, orbital cavity volume expanded with bony resorption, while eye globe volume decreased, contributing to an enophthalmic appearance. We observed no vertical displacement of the globe with aging.

Conclusion
This study provides a comprehensive overview of the multifaceted anatomical changes that occur in the periorbital region with aging. The insights gained from this research offer important clinical implications for addressing the signs of periorbital aging, guiding surgical interventions, and ultimately enhancing patient outcomes.

Level of Evidence IV
This journal requires that authors assign a level of evidence to each article. For a full description of these Evidence-Based Medicine ratings, please refer to the Table of Contents or the online Instructions to Authors www.springer.com/00266.
Sintering of inkjet-printed silver nanoparticles by large-area atmospheric pressure nitrogen plasma
Authors: Vida Julius , Solak Selen , Shao Yuhang , Homola Tomáš , List-Kratochvil Emil , Hermerschmidt Felix 
Published: January 2025

Abstract
Silver nanoparticles were sintered at atmospheric pressure using nitrogen plasma generated by diffuse coplanar surface barrier discharge (DCSBD). Compared to the standard thermal sintering of 1 h at 140 °C only 8 min of nonthermal plasma treatment at 50 °C were necessary to sufficiently anneal the silver nanoparticle film. Electrical resistivity as low as 9 × 10–6 Ω cm was achieved, i.e., 17% of the bulk conductivity of silver. X-ray photoelectron spectroscopy was used to study the removal of organic moieties from the surface of the inkjet-printed layers. Scanning electron microscopy revealed in detail the process of formation of interconnection between nanoparticles. Our findings pave the way for implementing low-cost and eco-friendly DCSBD plasma sintering into continuous roll-to-roll processing in the future for the annealing of silver nanoparticles on substrates that cannot tolerate high temperatures.
Soft stereolithographic 3D printed phantoms for dual-modality particle image velocimetry (PIV)
Authors: Hosseinzadeh Elnaz , Mirgolbabaee Hadi , van de Velde Lennart , Versluis Michel , Groot Jebbink Erik , Aguirre-Soto Alan , Reijnen Michel M. P. J. 
Published: January 2025

Abstract
The fabrication of arterial flow phantoms for fluid dynamics studies suitable for particle image velocimetry (PIV) techniques has presented challenges. Current 3D-printed blood flow phantoms with suitable transparency for optical PIV (laserPIV) are restricted to rigid materials far from those of arterial properties. Conversely, while soft 3D-printed phantoms demonstrate promise for sufficient acoustical transparency for ultrasound PIV (echoPIV), their optical translucency presents challenges for laserPIV applicability. This dual-modality approach leverages the high spatial resolution of laserPIV for in-vitro applications and the ability of echoPIV to quantify flow in both in-vivo and in-vitro application (also inside stents), providing a more comprehensive understanding of flow dynamics. In this study, we present a series of coated thin-walled 3D-printed compliant phantoms suitable for dual-modality PIV flow imaging (i.e., laserPIV and echoPIV) methods, overcoming current 3D-printable material limitations. Stereolithographic (SLA) 3D printing was used to fabricate pipe flow phantoms from a set of commercial soft resins (flexible and elastic) as vascular tissue surrogates. To overcome low transparency and poor surface finish of soft resins, we coated the 3D-printed flow phantoms with a soft, optically transparent, photo-activated polymeric coating. The feasibility of performing dual-modality PIV was tested in an in-vitro flow setup. Our results show that the average normalized root mean square errors obtained from comparing laserPIV and echoPIV velocity profiles against the analytical solutions were 3.2% and 5.1%, and 3.3% and 5.3% for the flexible and elastic phantoms, respectively. These results indicate that dual-modality PIV flow imaging is feasible in the 3D-printed coated phantoms, promoting its future use in fabricating clinically-relevant flow phantoms.

Graphical abstract
Sport practice and hemorrhoidal disease: results from a self-assessment questionnaire among athletes
Authors: Romano Lucia , Giuliani Antonio , Paniccia Federico , Masedu Francesco , Tersigni Leonardo , Padula Martina , Pietroletti Renato , Clementi Marco , Vistoli Fabio 
Published: January 2025

Abstract
Background
Hemorrhoidal disease (HD) is a common proctologic disease. Dietary and lifestyle play a role in the genesis of the disease or in its progression to more severe forms, although the exact mechanism is still not fully understood. We performed a pilot observational cross-sectional analytical association study to evaluate the possible association between sport activities and HD.

Methods
We included subjects aged 18 years old or more, competitive and non-competitive, practicing at least one sport activity, at least twice a week. Data were collected using an online questionnaire, developed on the Microsoft Teams communication platform.

Results
Out of the 312 study participants, 34% reported HD. Among subjects who practiced cycling or horseback riding, 57% reported suffering from HD; among those practicing bodybuilding, 48% complained of HD. In the multivariate logistic regression analysis, age and bodybuilding practice showed a statistically significant association with HD.

Conclusions
Some sport activities could play a role in the onset or worsening of HD. Our results showed a positive association between cycling, horseback riding, bodybuilding, and HD occurrence. Given the numerous health benefits of physical activity, patients should be provided with correct information regarding the practice of sports in relation to their pathology.
Early outcomes of robotic vs open living donor right hepatectomy in a US Center
Authors: Sambommatsu Yuzuru , Kumaran Vinay , Imai Daisuke , Savsani Kush , Khan Aamir A. , Sharma Amit , Saeed Muhammad , Cotterell Adrian H. , Levy Marlon F. , Lee Seung Duk , Bruno David A. 
Published: January 2025

Abstract

Background
Robotic living donor hepatectomy offers potential advantages but has been limited to high-volume centers, primarily in Asia and the Middle East. We report our experience establishing a robotic living donor right hepatectomy program in a U.S. center with low LDLT volume and no prior laparoscopic donor hepatectomy experience and analyze early outcomes.

Methods
This retrospective cohort study analyzed 37 living donor right hepatectomies (13 robotic [including one open conversion], 24 open) performed between June 2022 and February 2024.

Results
The robotic group had longer operative times (median [range], 451 [374–568] minutes vs 368 [276–421] minutes; P < 0.001) but less blood loss (median [range], 200 [50–700] mL vs 900 [300–2500] mL; P < 0.001). One case required unplanned open conversion due to gas embolism. Two hematomas/bleeding (Clavien–Dindo grade IIIB) occurred in the robotic group, but no biliary complications. Comprehensive Complication Index, liver function tests, and hospital stays were similar between the two groups, with no 90-day graft failure/mortality.

Conclusion
With extensive surgical experience in both open donor hepatectomy and robotic surgery, along with meticulous preparation as a team, U.S. centers with lower LDLT volume and no laparoscopic experience can safely implement robotic living donor right hepatectomy, achieving comparable short-term outcomes to the open approach. Further research on long-term outcomes and donor quality of life is necessary.
Paleoenvironmental constraints influencing charophyte communities in an Early Cretaceous Karstic Lake, La Pedrera de Meià Konservat-Lagerstätte (Southern Pyrenees): combining geochemical and palaeoecological analyses
Authors: Pérez-Cano Jordi , Gil-Delgado Alejandro , Oms Oriol , Mercedes-Martín Ramon 
Published: January 2025

Abstract
The combined sedimentological and palaeoecological analysis of charophyte-rich carbonate microfacies is proven to be a useful methodology for the palaeoenvironmental reconstruction of continental settings. However, the integration of these charophyte assemblages with geochemical proxies has not been explored, yet. In the present work, the charophyte-rich muddy carbonates of the Barremian La Pedrera de Meià Konservat-Lagerstätte (Southern Pyrenees, Spain) were studied from a taxonomic, taphonomic, and palaeoecological viewpoint, and the results were later integrated with previously published paleoenvironmental proxies (δ13C, δ18O, and elemental geochemistry). In the studied locality, monospecific Echinochara sp.–Charaxis spicatus meadows are only found in the temporary lakes recorded in the lower stages of lake infilling. The high Al, K, Fe and Ti concentrations and δ18O values previously described in these carbonates indicate meteoric infilling followed by evaporation periods. Freshwater and brackish communities are found in the upper stage of lake infilling. Freshwater settings had mixed meadows with Atopochara–Clavatoraxis, Clavatoroidae utricles–Clavatoraxis, and Ascidiella–Favargerella plants, while the brackish environments, that occurred laterally to freshwater, contain meadows dominated by porocharacean– Charaxis sp., and Echinochara sp.–Charaxis spicatus plants. Miliolids and broken dasycladaceans also occur in these facies. Sr/Ba ratios, REE + Y concentrations, and δ18O values previously reported in these lacustrine carbonates indicate prevalence of freshwater environments with occasional marine influence, which agrees with the occurrence of distinct brackish and freshwater charophyte assemblage and marine-affinity organisms, suggesting that during the last stage of lake evolution, the system was part of a coastal wetland. This work demonstrates that the integration of geochemical, palaeontological and palaeoecological datasets improves the understanding of the fossil continental carbonate settings and their evolution.
Delayed cutaneous wound healing in young and old female mice is associated with differential growth factor release but not inflammatory cytokine secretion
Authors: Plum Melissa , Beier Justus P. , Ruhl Tim 
Published: January 2025

Abstract
The capacity for tissue repair during wound healing declines with age. A chronic low but systemic inflammatory status, often called “inflammaging”, is considered a key factor that contributes to impaired tissue regeneration. This phenomenon has been substantiated by an increased number of immune cells in wound-tissue of old mice. Although immune cells coordinate an inflammatory response by their secretome the composition of the wound milieu has not been examined. In young (2 months) and old (18 months) female mice, excision wounds were induced using a punch biopsy device, i.e., the healing progress occurred through secondary intention. The closure rate was analyzed for 7 days. At days 1, 3 and 7 post-surgery, wound specimen were investigated for immunohistochemical detection of granulocytes, M1-macrophages and mesenchymal stem cells of the skin. The concentrations of inflammatory cytokines and regenerative growth factors were determined in tissue homogenates by ELISA. The carbonyl assay was used to determine protein oxidation. In old mice, the wound closure was delayed between days 1 and 3 post-surgery, as was the peak of immune cell infiltration. There was no age effect on the concentration of inflammatory cytokines, but wounds of young animals contained higher number of mesenchymal stem cells and increased levels of growth factors. Protein oxidation was increased with age. The present study suggests that a reduced regenerative capacity rather than an enhanced inflammatory score affected the tissue regeneration process in old mice.
Estrogen receptor positivity in stromal cells of the tumor bed predicts the response to neoadjuvant chemotherapy for breast carcinoma
Authors: Bayram Aysel , Bagbudar Sidar , Karanlık Hasan , Cabıoglu Neslihan , Aydıner Adnan , Onder Semen , Yavuz Ekrem 
Published: January 2025

Abstract
Purpose
This study aimed to determine estrogen receptor (ER) expression in stromal cells in postchemotherapy tumor bed (PCTB) and its relationship with tumor regression and tumor characteristics.

Methods
The study included 490 breast cancer patients who received neoadjuvant chemotherapy (NAC). We performed ER in stromal cells in all resection specimens and available pre-treatment core biopsy materials of 299 patients immunohistochemically.

Results
Two hundred and forty-two (49.4%) cases were negative for ER in the stromal cells of the PCTB, and 248 (50.6%) cases were positive. ER-positive stromal cells in the PCTB correlated with a higher regression rate (90.2 vs 68.6%) and lower mean residual cancer burden value (1.366 vs 2.424) compared to ER-negative cases (p < 0.001). Stromal ER positivity was more prevalent in cases achieving pathologic complete response (pCR) (68.1%) compared to those without pCR (39.8%, p < 0.001). ER positivity in stromal cells was more common in non-luminal tumors than in luminal ones. Multivariate analysis identified stromal ER positivity (OR: 3.059, 95% CI [1.947–4.807], p < 0.001), intrinsic subtype (Odds ratio (OR): 1.477, 95% confidence interval (CI) [1.102–1.980], p = 0.009), and Ki67 index (OR: 1.028, 95% CI [1.104–1.041], p < 0.001) as independent predictors of pCR. In core biopsies before NAC, 270 cases (90.3%) and 29 cases (9.7%) were negative and positive in stromal cells, respectively. Out of the cases with ER-negativity in stromal cells before NAC, 132 (48.9%) converted to ER positivity in stromal cells of PCTB and displayed a high regression rate (89.8%).

Conclusion
This is the first study regarding ER expression in the stroma of breast carcinoma that compares treatment response after NAC. We showed that the increase in ER positivity in the stromal cells of the PCTB is correlative with the complete response and tumor subtypes. In this manner, ER positivity in stromal cells will soon serve as a cornerstone for individualized treatment options.
Cardiotoxicity as important differential diagnosis for reduced myocardial blood flow during Rubidium cardiac PET/CT
Authors: Pop Sabin G. , Hägler Eva , Popescu Cristina , Burger Irene A. , Sauter Alexander W. 
Published: January 2025

AbstractA 65-year-old woman with a history of ductal mammary carcinoma and recent autonomic dysfunction underwent a Rb-82 chloride (RbCl) cardiac PET/CT scan that showed no ischemia or scarring, but significantly reduced myocardial flow reserve (MFR) (global: 1.5) and a CAC-Score of 0. The patient’s chemotherapy history (paclitaxel, carboplatin, epirubicin, pembrolizumab 2 years before) with elevated Troponin T and NT-pro-BNP levels at that time, and now reduced MFR with 0 CAC suggests cancer-therapy-related cardiotoxicity. An important differential diagnosis to the more common CAD-associated microvascular disease. Furthermore, tumor recurrence with a PET-avid lymph node metastasis was found additionally.