bluuebunny/crossref_metadata_2025_split huggingface dataset:
Crohn’s patients and healthy infants share immunodominant B cell response to commensal flagellin peptide epitopes
Authors: Zhao Qing , Duck Lennard Wayne , Killian John T. , Rosenberg Alexander F. , Mannon Peter J. , Glenn King R. , Denson Lee A. , Kugathasan Subra , Janoff Edward N. , Jenmalm Maria C. , Elson Charles O. 
Published: August 2023

AbstractAbout half of patients with Crohn’s disease (CD) develop selective serum IgG response to flagellin proteins of theLachnospiraceaefamily. Here, we identified a dominant B cell peptide epitope in CD, locating in the highly conserved “hinge region” between the D0 and D1 domains at the amino-terminus ofLachnospiraceaeflagellins. Serum IgG reactive to this epitope is present at an elevated level in adult CD patients and in pediatric CD patients at diagnosis. Most importantly, high levels of serum IgG to the hinge epitope were found in most infants from 3 different geographic regions (Uganda, Sweden, and the USA) at one year of age. This vigorous homeostatic response decrements with age as it is not present in healthy adults. These data identify a distinct subset of CD patients, united by a shared reactivity to this dominant flagellin epitope that may represent failure of a homeostatic response beginning in infancy.
A cortico-subcortical loop for motor control via the pontine reticular formation
Authors: Bősz Emília , Plattner Viktor M. , Biró László , Kóta Kata , Diana Marco A. , Acsády László 
Published: August 2023

SummaryMovement and locomotion are controlled by large neuronal circuits like the cortex-basal ganglia (BG)-thalamus loop. Inhibitory output of the BG loop can directly control movement via specialized connections with the brainstem. Whether other parallel loops with similar logic exist is presently unclear. Here we demonstrate that glycine transporter 2-positive (GlyT2+) cells of the pontine reticular formation (PRF) receive cortical inputs and in turn innervate the thalamus. Thalamus-projecting GlyT2+ cells innervate subcortical regions distinct from BG targets. Cortical cells co-innervate PRF/GlyT2+ cells and thalamus as in the BG loops. Cortex exerts strong excitatory control on PRF/GlyT2+ cells and these neurons powerfully inhibit their thalamic targets. Activation of thalamus projecting PRF/GlyT2+ cells leads to contralateral turning. These results demonstrate that the PRF is part of a cortico-subcortical loop that regulates motor activity parallel to the BG circuits. The cortico-PRF-thalamus loop can synergistically control turning with the BG loops via distinct descending pathways.Graphical abstract
Dietary cystine restriction increases the proliferative capacity of the small intestine of mice
Authors: de Jong Judith C.W. , van Rooijen Kristel S. , Stigter Edwin C.A. , Can Gülersönmez M. , de Zoete Marcel R. , Top Janetta , Baars Matthijs J.D. , Vercoulen Yvonne , Kuipers Folkert , van Mil Saskia W.C. , Ijssennagger Noortje 
Published: August 2023

AbstractOver 88 million people are currently estimated to have adopted towards a vegan or vegetarian diet. Cysteine is a semi-essential amino acid, which availability is largely dependent on dietary intake of meat, eggs and whole grains. Vegan/vegetarian diets are therefore inherently low in cysteine concentrations. Sufficient uptake of cysteine is crucial, as it serves as substrate for protein synthesis and conversion to taurine and glutathione. In this study, we therefore investigate the effect of low dietary cystine, the oxidized derivative of cysteine, on intestinal epithelial layer function. Mice (8/group) received a high fat diet with normal or low cystine concentration for 2 weeks. We observed no changes in plasma methionine, cysteine, taurine or glutathione levels after 2 weeks. Stem cell markers as well as the proliferation markerKi67were increased upon cystine restriction in the small intestine. In line with this, gene set enrichment analysis indicated enrichment of Wnt signaling in the small intestine of mice on the low cystine diet, indicative of proliferative cells. Increased proliferation was absent in the colon. In the colon, dietary cystine restriction results in an increase in goblet cells, but no significant changes in the thickness of the mucus barrier or in its protective capacity. Also the microbiome was not changed upon dietary restriction. In conclusion, we show that cystine restriction for two weeks does not seem to induce any systemic effects. The increased proliferative capacity and number of goblet cells observed in the intestine may be the effect of starting epithelial damage or a reaction of the epithelium to start enlarging the absorptive capacity.
The RIDD activity ofC. elegansIRE1 modifies neuroendocrine signaling in anticipation of environment stress to ensure survival
Authors: Ying Mingjie , Argon Yair , Gidalevitz Tali 
Published: August 2023

AbstractXbp1splicing and regulated IRE1-dependent RNA decay (RIDD) are two RNase activities of the ER stress sensor IRE1. WhileXbp1splicing has important roles in stress responses and animal physiology, the physiological role(s) of RIDD remain enigmatic. Genetic evidence inC. elegansconnects XBP1-independent IRE1 activity to organismal stress adaptation, but whether this is via RIDD, and what are the targets is yet unknown. We show that cytosolic kinase/RNase domain ofC. elegansIRE1 is indeed capable of RIDD in human cells, and that sensory neurons use RIDD to signal environmental stress, by degrading mRNA of TGFβ-like growth factor DAF-7.daf-7was degraded in human cells by both human and worm IRE1 RNAse activity with same efficiency and specificity asBlos1,confirmingdaf-7as RIDD substrate. Surprisingly,daf-7degradationin vivowas triggered by concentrations of ER stressor tunicamycin too low forxbp-1splicing. Decrease in DAF-7 normally signals food limitation and harsh environment, triggering adaptive changes to promote population survival. BecauseC. elegansis a bacteriovore, and tunicamycin, like other common ER stressors, is an antibiotic secreted byStreptomyces spp., we asked whetherdaf-7degradation by RIDD could signal pending food deprivation. Indeed, pre-emptive tunicamycin exposure increased survival ofC. eleganspopulations under food limiting/high temperature stress, and this protection was abrogated by overexpression of DAF-7. Thus,C. elegansuses stress-inducing metabolites in its environment as danger signals, and employs IRE1’s RIDD activity to modulate the neuroendocrine signaling for survival of upcoming environmental challenge.
Target-aware Variational Auto-encoders for Ligand Generation with Multimodal Protein Representation Learning
Authors: Ngo Nhat Khang , Hy Truong Son 
Published: August 2023

AbstractWithout knowledge of specific pockets, generating ligands based on the global structure of a protein target plays a crucial role in drug discovery as it helps reduce the search space for potential drug-like candidates in the pipeline. However, contemporary methods require optimizing tailored networks for each protein, which is arduous and costly. To address this issue, we introduceTargetVAE, a target-aware variational auto-encoder that generates ligands with high binding affinities to arbitrary protein targets, guided by a novel multimodal deep neural network built based on graph Transformers as the prior for the generative model. This is the first effort to unify different representations of proteins (e.g., sequence of amino-acids, 3D structure) into a single model that we name asProtein Multimodal Network(PMN). Our multimodal architecture learns from the entire protein structures and is able to capture their sequential, topological and geometrical information. We showcase the superiority of our approach by conducting extensive experiments and evaluations, including the assessment of generative model quality, ligand generation for unseen targets, docking score computation, and binding affinity prediction. Empirical results demonstrate the promising performance of our proposed approach. Our software package is publicly available athttps://github.com/HySonLab/Ligand_Generation.
The effects of economic difficulties on social and health care costs of children –target trial emulation using complete birth cohort data in Finland
Authors: Hiilamo Aapo , Keski-Säntti Markus , Juutinen Aapo , Mäkinen Lauri , Ristikari Tiina , Lallukka Tea 
Published: August 2023

AbstractIt is unclear how much costs economic difficulties incur to the health and social care sector, which is a critical research gap to support the economic case for preventing child poverty. We examine the health and social service costs due to families entering into, and transitioning out of, social assistance used as a proxy measure for economic difficulties. We analyzed register data on all Finnish children born in 1997 in the framework of a non-randomized target trial. Inverse probability treatment weighting techniques were used to make the comparison group similar to the treatment group in terms of 29 pretreatment variables. Entry to social assistance was associated with some 1398-2591€ (50%) higher cumulative health and social care costs of the children three years after their families transitioned to social assistance, compared to the group that did not enter to social assistance system. This difference was primarily attributed to higher social care costs. Continued social assistance use was associated with some 1018-2775€ (31%) higher costs compared to the comparison group that exited social assistance. These findings support an economic argument to prevent families from entering economic difficulties and to help those in such situations to transition out.
Unraveling the link between Neuropathy Target Esterase NTE/SWS, lysosomal storage diseases, inflammation, and abnormal fatty acid metabolism
Authors: Tsap Mariana I. , Yatsenko Andriy S. , Hegermann Jan , Beckmann Bibiana , Tsikas Dimitrios , Shcherbata Halyna R. 
Published: August 2023

ABSTRACTMutations inDrosophilaSwiss Cheese (SWS) gene or its vertebrate orthologue Neuropathy Target Esterase (NTE) lead to progressive neuronal degeneration in flies and humans. Despite its enzymatic function as a phospholipase is well-established, the molecular mechanism responsible for maintaining nervous system integrity remains unclear. In this study, we found that SWS is present in surface glia that form the blood-brain-barrier (BBB) and that SWS is important to maintain its structure and permeability. Importantly, BBB glia-specific expression ofDrosophilaSWS or human NTE in theswsmutant background fully rescues surface glial organization and partially restores BBB integrity, suggesting a conserved function of NTE/SWS. Interestingly,swsmutant glia showed abnormal organization of plasma membrane domains and tight junction rafts accompanied by the accumulation of lipid droplets, lysosomes, and multilamellar bodies. Since the observed cellular phenotypes closely resemble the characteristics described in a group of metabolic disorders known as lysosomal storage diseases (LSDs), our data established a novel connection between NTE/SWS and these conditions. We found that mutants with defective BBB exhibit elevated levels of fatty acids, which are precursors of eicosanoids and are involved in the inflammatory response. Also, as a consequence of a permeable BBB, several innate immunity factors are upregulated. Treatment with anti-inflammatory agents prevents the abnormal architecture of the BBB, suggesting that inflammation contributes to the maintenance of a healthy brain barrier. Since a defective BBB is associated with many neurodegenerative diseases, a better understanding of the molecular mechanisms of inflammation may help to promote the use of anti-inflammatory therapy for age-related neurodegeneration.
AePUbpromoter length modulates gene expression inAedes aegypti
Authors: Anderson Michelle A.E. , Leftwich Philip T. , Wilson Ray , Carabajal Paladino Leonela Z. , Basu Sanjay , Rooney Sara , Adelman Zach N. , Alphey Luke 
Published: August 2023

AbstractMolecular tools for modulating transgene expression inAedes aegyptiare few. Here we demonstrate that adjustments to theAePUbpromoter length can alter expression levels of two reporter proteins inAe. aegypticell culture and in mosquitoes. This provides a simple means for increasing or decreasing expression of a gene of interest and easy translation from cells to whole insects.
Short oligomers rather than rings of human RAD52 promote single-strand annealing
Authors: Kharlamova Maria A. , Kushwah Manish S. , Jachowski Tobias J. , Subramaniam Sivaraman , Stewart A. Francis , Kukura Philipp , Schäffer Erik 
Published: August 2023

ABSTRACTGenome maintenance and stability rely on the repair of DNA double-strand breaks. The break repair can be mediated by the single-strand annealing protein RAD52. RAD52 forms rings that are thought to promote annealing. However, RAD52’s annealing activity decreases with increasing concentrations that favor ring formation. Thus, which oligomeric form and how RAD52 anneals DNA strands and detects sequence homology is unclear. We combine mass photometry with biochemical assays to quantify oligomeric states of human RAD52 with and without DNA and put forward an alternative mechanism illustrating the critical role of short oligomers for single-stranded DNA annealing. We found that while truncated RAD52 formed undecameric rings at nanomolar concentrations, full-length RAD52 was mostly monomeric at lower nanomolar, physiological concentrations. At higher concentrations, it formed rings with a variable stoichiometry from heptamers to tridecamers. At low concentrations, with hardly any rings present, RAD52 already promoted single-strand annealing. Rings and short oligomers could bind at least two single DNA strands, but if complementary strands were both bound to rings annealing was inhibited. Our findings suggest that single-strand annealing and homology detection is mediated by short oligomers of RAD52 instead of rings.
Vicenarian of workplace incivility: a bibliometric analysis and systematic review
Authors: Gupta Aditi , Chaudhuri Ranjan , Apoorva Apoorva , Chaudhary Swati , Thrassou Alkis , Sakka Georgia , Grandhi Balakrishna 
Published: August 2023

PurposeThe purpose of this paper is twofold: first is to map and analyse prominent contributions, current dynamics, patterns, gaps and research prospects in the field of workplace incivility (WI); second is to provide a coherent theoretical research framework for future research.Design/methodology/approachThis study adopts a two-step analysis approach by combining bibliometric analysis and systematic literature review to explore the research topic of WI, besides, using multiple methodologies including bibliometric, network and content analyses.FindingsThis study found that L. M. Cortina and M. S. Hershcovis are the top two most influential authors among all authors in the sample publications. Journal of Occupational Health Psychology is one of the top-ranking journal that often publishes this topic of articles. USA and The Bowling Green State University are the most influential country and institutions, respectively. Besides, burnout and retention are also common keywords were identified based on keywords co-occurrence map, showing that WI has a major impact on burnout and employee retention. Based on the above analysis, this paper constructed a theoretical research framework of WI.Research limitations/implicationsThis paper only draw data from one database—Scopus—which cannot provide broad coverage of the research topic. WI research trends and trajectories may be assessed to enable academics and practitioners better understand the current and future trends and research directions. Future studies in this field might use the findings as a starting point to highlight the nature of the topic.Originality/valueThis study is the first to use an systematic literature review (SLR) to evaluate the association between WI and other organizational behaviour. The study contributions are fourfold, extending the work and overcoming the methodologies of prior research that only focussed on characteristics of incivility in nursing. In addition, this paper presents an in-depth analysis of this topic and provides a comprehensive theoretical research framework for future study.
Forecast combination using grey prediction with fuzzy integral and time-varying weighting in tourism
Authors: Hu Yi-Chung 
Published: August 2023

PurposeTourism demand forecasting is vital for the airline industry and tourism sector. Combination forecasting has the advantage of fusing several forecasts to reduce the risk of inappropriate model selection for analyzing decisions. This paper investigated the effects of a time-varying weighting strategy on the performance of linear and nonlinear forecast combinations in the context of tourism.Design/methodology/approachThis study used grey prediction models, which did not require that the available data satisfy statistical assumptions, to generate forecasts. A quality-control technique was applied to determine when to change the combination weights to generate combined forecasts by using linear and nonlinear methods.FindingsThe empirical results showed that except for when the Choquet fuzzy integral was used, forecast combination with time-varying weights did not significantly outperform that with fixed weights. The Choquet integral with time-varying weights significantly outperformed that with fixed weights for all model combinations, and had a superior forecasting accuracy to those of other combination methods.Practical implicationsThe tourism sector can benefit from the use of the Choquet integral with time-varying weights, by using it to formulate suitable strategies for tourist destinations.Originality/valueCombining forecasts with time-varying weights may improve the accuracy of the predictions. This study investigated incorporating a time-varying weighting strategy into combination forecasting by using CUSUM. The results verified the effectiveness of the time-varying Choquet integral for tourism forecast combination.
FinTech credit: uncovering knowledge base, intellectual structure and research front
Authors: Thomas Nisha Mary , Mendiratta Priyam , Kashiramka Smita 
Published: August 2023

PurposeOwing to the dramatic rise of FinTech credit in the financial sector, this study describes its knowledge and intellectual structure and paves the way for future research.Design/methodology/approachThe study employs citation analysis, keyword analysis, co-author analysis, co-citation analysis and bibliographic coupling on 268 peer-reviewed articles published during 2010–2021 and extracted from the Web of Science database.FindingsResearch on FinTech credit has picked up momentum from 2016, with majority contributions from China, followed by UK and USA. International Journal of Bank Marketing is found to be the most productive journal. Co-citation analysis reveals that past studies have focused on three dominant themes, viz. (a) factors that influence user intention to adopt technological products and services (b) borrowers' and lenders' characteristics that impact fund-raising in FinTech credit platforms and (c) evolution of FinTech market over the years. Bibliographic coupling reveals that recent trends in FinTech credit include (a) impact of emerging technologies like blockchain, artificial intelligence, big data on financial system, (b) factors that encourage consumers to adopt the FinTech products and services, (c) mechanisms by which FinTechs have transformed formal credit markets, (d) factors that lead to successful fundraising in FinTech platforms and (e) critical perspectives on digital lending platforms.Originality/valueTo the best of the authors' knowledge, this is a pioneering study undertaking an exhaustive analysis of FinTech credit as a research area. The study offers valuable insights on potential topics of research in FinTech credit domain like investigating Balance Sheet Lending Model, investigating the impact of FinTechs on financial system, and new markets by collaborating with scholars of other regions.
Green food packages’ effects on consumers’ pre- to post-consumption evaluations of restaurant curbside pickup service
Authors: Kwok Linchi , Lin Michael S. 
Published: August 2023

Purpose
This study aims to assess green food packages’ role in sustaining a restaurant’s curbside pickup service on three stages of consumer experiences: choosing a restaurant, evaluating their experiences of a recent purchase and weighing their post-consumption behavioral intentions after the recent purchase.

Design/methodology/approach
The service encounters framework and relevant literature guided the development of the questionnaire. A Qualtrics panel data of 314 valid questionnaires were collected and analyzed with choice experience, ordinary least squares regression and PROCESS modeling.

Findings
First, word-of-mouth (WOM) and function encounters significantly influence consumers’ first-time curbside pickup purchasing decisions. Then, service results encounter (besides distributor encounter) most significantly affects consumers’ overall curbside pickup experience. Finally, green food packages increase consumers’ shares of future purchases through their positive WOM intentions and extra efforts of revisiting the restaurant. Consumers’ perceived importance of green restaurant practices strengthens green food packages’ positive impact on extra efforts.

Practical implications
This study provides operational and marketing insights for restaurants to use food packages and sustain their curbside pickup service.

Originality/value
Besides assessing consumers’ evaluations and behavioral intentions for an off-premises restaurant service expected to stay beyond the pandemic, this research uniquely focuses on green food packages, a sustainability issue lacking research attention. The findings add new empirical insights to studies about sustainability and restaurant/food–retail operations.
Linking social media marketing to restaurant performance – the moderating role of advertising expenditure
Authors: Han Wenjia , Ozdemir Ozgur , Agarwal Shivam 
Published: August 2023

PurposeBuilt upon customer engagement marketing theory and uses and gratification theory, this study examines the link between individual social media marketing (SMM) performance indicators and restaurant sales performance at the firm level. Moreover, the study investigates the moderating effect of advertising expenditure on this proposed relationship.Design/methodology/approachRandom effect regression models were developed in Stata to examine the associations between SMM performance indicators, advertising expenditure, and restaurant firm revenue. Twelve years of SMM data from brands' Facebook pages were collected with a web scraper built in Python. Natural language processing was used to analyze the sentiment of user-generated content (UGC).FindingsThe results suggest that restaurant annual sales revenue increases as the volume of brand posts, “like”s, “share”s and positive comments on restaurants' Facebook pages increase. However, the total number of comments and the number of negative comments show non-significant associations with revenue. Firm advertising expenditure negatively moderates the relationships between sales revenue and the number of “like”s, “share”s, total comments and positive comments.Practical implicationsRestaurants benefit from making frequent posts on SNSs. Promotions that motivate online users to “like”, share, and comment on brand posts should be implemented. Firms with limited advertising budgets are encouraged to actively create buzz on SNSs due to evidenced stronger effects of UGC on sales performance than large advertisers.Originality/valueThis research bridges the gap by studying the effects of individual SMM performance indicators on restaurant financial outcomes. The findings support the effectiveness of SMM; and, for the first time, demonstrate that SMM could generate a more profound impact for firms with low advertising budgets.