bluuebunny/crossref_metadata_2025_split huggingface dataset:
Impact of pre‐ and post‐diagnosis physical activity on the mortality of patients with cancer: Results from the Health Examinees‐G study in Korea
Authors: Choi Jaesung , Park Joo‐Yong , Kim Ji‐Eun , Lee Miyoung , Lee Kyuwan , Lee Jong‐Koo , Kang Daehee , Shin Aesun , Choi Ji‐Yeob 
Published: June 2023

AbstractBackgroundPhysical activity (PA) is recommended to improve the survival of cancer patients. However, the prognostic impact of specific PAs is not well understood. Therefore, we investigated the associations of the duration, type, intensity, and number of PAs one participates in pre‐ and post‐diagnosis with mortality in Korean patients with cancer.MethodsAmong the participants aged 40–69 years recruited from the Health Examines study, those diagnosed with cancer after baseline (n = 7749) and within 10 years before baseline (n = 3008) were included in the analyses for pre‐ and post‐diagnosis PA, respectively. Duration, intensity, type, and number of leisure‐time physical activities participated in were assessed using questionnaires. The Cox proportional hazard model was used to characterize the association between PA and cancer‐specific mortality, adjusting for demographics, behaviors, comorbidities, and cancer stage based on the Surveillance, Epidemiology, and End Results program.ResultsPre‐diagnosis, patients participating in vigorous‐intensity activities (hazard ratio [HR]: 0.70, 95% confidence interval [CI]: 0.61–0.82), walking (HR: 0.85, 95% CI: 0.74–0.97), climbing (HR: 0.65, 95% CI: 0.55–0.77), sports (HR: 0.39, 95% CI: 0.25–0.61), and more than two activities (HR: 0.73, 95% CI: 0.63–0.86) had significantly lower all‐cause mortality. Importantly, these associations were only found in patients with colorectal cancer participating in vigorous‐intensity activities (HR: 0.40, 95% CI: 0.23–0.70). Post‐diagnosis, only patients who performed more than two activities (HR: 0.65, 95% CI: 0.44–0.95) had significantly lower all‐cause mortality. Similar associations were found for cancer mortality, both pre‐ and post‐diagnosis.ConclusionSpecific characteristics of PA pre‐ and post‐diagnosis may influence the survival of cancer patients.
Analyses of Elaeocarpus sphaericus Extract for Antioxidant, Antiproliferative and Gene Repression Activities against HIF‐1α and VEGF
Authors: Joshi Swati , Painuli Sakshi , Misra Kshipra , Kumar Navin , Bachheti Rakesh Kumar , Rai Nishant , Singab Abdel Nasser B. 
Published: June 2023

AbstractThe study presents antioxidant, phytochemical, anti‐proliferative, and gene repression activities against Hypoxia‐inducible factor (HIF‐1) alpha and Vascular endothelial growth factor (VEGF) of Elaeocarpus sphaericus extract. Elaeocarpus sphaericus dried and crushed plant leaves were extracted using water and methanol by ASE (Accelerated Solvent Extraction) method. Total phenolic content (TPC) and total flavonoid content (TFC) were used to measure the extracts′ phytochemical activity (TFC). Antioxidant potential of the extracts was measured through DPPH, ABTS, FRAP, and TRP. Methanolic extract of the leaves of E. sphaericus has shown a higher amount of TPC (94.666±4.040 mg/gm GAE) and TFC value (172.33±3.21 mg/gm RE). The antioxidant properties of extracts in the yeast model (Drug Rescue assay) showed promising results. Ascorbic acid, gallic acid, hesperidin, and quercetin were found in the aqueous and methanolic extracts of E. sphaericus at varying amounts, according to a densiometric chromatogram generated by HPTLC analysis. Methanolic extract of E. sphaericus (10 mg/ml) has shown good antimicrobial potential against all bacterial strains used in the study except E. coli. The anticancer activity of the extract in HeLa cell lines ranged from 77.94±1.03 % to 66.85±1.95 %, while it ranged from 52.83±2.57 % to 5.44 % in Vero cell lines at varying concentration (1000 μg/ml–31.2 μg/ml). A promising effect of extract was observed on the expression activity of HIF‐1 and VEGF gene through RT‐PCR assay.
Secondary Metabolite Analysis of Phomopsis sp. LGT‐5 Based on the Dual Guidance of Whole‐Genome Sequencing and HPLC‐Q‐ToF‐MS/MS
Authors: Zhu Jia‐Jia , Xi Na , Liu Cheng , Ma Ping‐Yang , Wu Xin‐Yuan , Zhou Li , An Zhen‐Yu , Jiang Zhi‐Bo , Wu Xiu‐Li , Wang Jian‐Huan 
Published: June 2023

AbstractMicroorganisms produce a wealth of structurally diverse specialized metabolites with a remarkable range of biological activities. The Phomopsis sp. LGT‐5 was obtained through tissue block and repeatedly crossed methods from Tripterygium wilfordii Hook. F. The antibacterial experiments of LGT‐5 showed that it has high inhibitory activity against Staphylococcus aureus and Pseudomonas aeruginosa, and moderate inhibitory activity against Candida albicans. To research the generation of the antibacterial phenomenon of LGT‐5 and provide support for further research and application, the whole genome sequencing (WGS) of LGT‐5 was obtained by single‐molecule real‐time DNA sequencing platform Pacific Biosciences (PacBio) sequencing and Illumina paired‐end sequencing. The final assembled LGT‐5 genome is 54.79 Mb with a contig N50 of 290.07 kb; in addition, its secondary metabolites were detected through HPLC‐Q‐ToF‐MS/MS. By comparing its MS/MS data, the secondary metabolites were analyzed based on visual network maps obtained on the Global Natural Products Social Molecular Networking (GNPS). The analysis results showed that the secondary metabolites of LGT‐5 were triterpenes and various cyclic dipeptides.
Insights into the Structural and Energetic Descriptions of Ubiquitin Specific Protease 7 (USP7) Catalytic Mechanisms by Hybrid QM/MM Simulations.
Authors: Velázquez‐Libera José Luis , Caballero Julio , Alzate‐Morales Jans , Ruiz‐Pernía J. Javier , Tuñón Iñaki 
Published: June 2023

AbstractWe have reported the first computational study about USP7 reaction mechanism with the substrate Ubiquitin‐Rhodamine 110‐G (Ub‐Rho) using a robust methodology that integrated homology modeling, classical molecular dynamics (MD) simulations, protein‐protein interaction fingerprints (IFPs) analysis, principal components analysis (PCA) and clustering, and the hybrid QM/MM simulations using the adaptive string method (ASM). The results presented in this work could be useful to understand the dynamic nature of USP7 enzyme‐substrate complexes. In this sense, we have provided a detailed structural description of the most relevant conformational changes observed in our simulations, which could be used as reference for modeling more complicated USP7’s enzyme‐substrate complexes. On the other hand, our descriptions of the protein‐protein interactions of Ub‐Rho's residues at P5 to P1’ positions with the CD's active site, and the geometries of the stationary states located along the MFEP could be considered for designing new potent and selective inhibitors for USP7’s activity.
Coordination Behavior of the Tellurium Incorporated Mercuraazametallamacrocycle and Investigation of d10⋅⋅⋅d10 Interactions between Closed Shell (Ag+ Hg2+) Metal Ions
Authors: Raju Saravanan , Singh Harkesh B. , Kumar Sangit , Butcher Ray J. 
Published: June 2023

AbstractHerein, a new tellurium and mercury containing mercuraazametallamacrocycle has been prepared via (2+2) condensation of bis(o‐aminophenyl)telluride and bis(o‐formylphenyl)mercury(II). The isolated bright yellow solid of mercuraazametallamacrocycle has adopted unsymmetrical figure‐of‐eight conformation in the crystal structure. To study the metallophilic interactions between closed shell metal ions, the macrocyclic ligand has been treated with two equiv. of AgOTf (OTf=trifluoromethansulfonate) and AgBF4, which afforded greenish‐yellow bimetallic silver complexes. The isolated silver complexes displayed intramolecular Hg⋅⋅⋅Ag, Te⋅⋅⋅Ag interactions as well as intermolecular Hg⋅⋅⋅Hg interactions and formed an extended 1D molecular chain by directing six atoms to interact as TeII⋅⋅⋅AgI⋅⋅⋅HgII⋅⋅⋅HgII⋅⋅⋅AgI⋅⋅⋅TeII in a non linear fashion. The Hg⋅⋅⋅Ag, Te⋅⋅⋅Ag interactions have also been studied in solution by 199Hg, 125Te NMR spectroscopy, absorption, and emission spectroscopy. In DFT calculations, the Atom in Molecule (AIM) analysis, non‐covalent interactions (NCI), natural bonding orbital (NBO) analysis strongly supported for experimental evidences and revealed that the intermolecular Hg⋅⋅⋅Hg interaction is stronger than the intramolecular Hg⋅⋅⋅Ag interactions.
The French approach to entrepreneurial failure: A profound paradigm shift
Authors: Lemercier Karine 
Published: June 2023

AbstractFrom the long‐standing disapproval attached to the "bankruptcy" of the entrepreneur, through the contested recognition of the right not to pay his debts, the French law of insolvency has evolved to gradually take into account the fate of the man in entrepreneurial failure. Thus, the objective of the rebound of the entrepreneur is part of the reforms of the law of companies in difficulty for several years: introduction of professional recovery leading to the cancellation of debts, acceleration of the judicial liquidation procedure, limitation of the scope of the guarantees offered to creditors and reduction of the weight of sanctions. Beyond the measures, the evolution of the law of companies in difficulty reflects a real mutation in the French perception of entrepreneurial failure. A paradigm shift is taking place where the culture of failure gradually gives way to a culture of rebound, so that the entrepreneur can be entitled to a "second chance". The article focuses on the legal mechanisms of the rebound of the individual entrepreneur after a judicial liquidation under French law. The objective is to demonstrate that a paradigm shift is taking place where the culture of failure is gradually giving way to a culture of rebound. If the change has appeared for 30 years, it appears more blatantly for 3 years, whether in European or French law. However, many economic stigmas persist, such as indicators in the banking file of companies or data on judicial liquidation that remain publicly available. In order to promote the rebound of the entrepreneur, we propose in particular the establishment of a right to be forgotten.
The surrogate scaffold method for quantifying molecular release kinetics from drug delivery systems
Authors: Borges Fernando T. P. , Papavasiliou Georgia , Teymour Fouad 
Published: June 2023

AbstractThe accurate determination of kinetics of therapeutic release from drug delivery vehicles is an essential step in the optimized design of such systems for biomedical and pharmaceutical applications. Most methods in current use for quantifying therapeutic release rates are developed to provide consistency, reproducibility, and ease of usage in a laboratory setting. These methods, however, do not necessarily mirror the release conditions when the drug delivery system comes into contact with the target tissue environment during application. As a result, the findings from these studies provide only comparative guidelines about the drug delivery rates and duration. Successful optimization of a drug delivery system requires complete, and accurate, knowledge about the release profile over an extended period of time to determine the initial release rate—including burst release if present, the rate of change of the release kinetics, and the maximum duration of delivery at a minimum therapeutic concentration level. We have developed an indirect method for the quantification of release kinetics suitable for nanoparticle‐based drug delivery systems that utilizes a hydrogel scaffold as a tissue surrogate to better emulate therapeutic delivery into a target tissue environment. Details of the method and its application to the release of an angiogenic peptide from a nanoparticle emulsion are provided in this communication.
Integrated metabolic and genetic analysis reveals distinct features of human differentiated thyroid cancer
Authors: Cararo Lopes Eduardo , Sawant Akshada , Moore Dirk , Ke Hua , Shi Fuqian , Laddha Saurabh , Chen Ying , Sharma Anchal , Naumann Jake , Guo Jessie Yanxiang , Gomez Maria , Ibrahim Maria , Smith Tracey L. , Riedlinger Gregory M. , Lattime Edmund C. , Trooskin Stanley , Ganesan Shridar , Su Xiaoyang , Pasqualini Renata , Arap Wadih , De Subhajyoti , Chan Chang S. , White Eileen 
Published: June 2023

AbstractBackgroundDifferentiated thyroid cancer (DTC) affects thousands of lives worldwide each year. Typically, DTC is a treatable disease with a good prognosis. Yet, some patients are subjected to partial or total thyroidectomy and radioiodine therapy to prevent local disease recurrence and metastasis. Unfortunately, thyroidectomy and/or radioiodine therapy often worsen(s) quality of life and might be unnecessary in indolent DTC cases. On the other hand, the lack of biomarkers indicating a potential metastatic thyroid cancer imposes an additional challenge to managing and treating patients with this disease.AimThe presented clinical setting highlights the unmet need for a precise molecular diagnosis of DTC and potential metastatic disease, which should dictate appropriate therapy.Materials and methodsIn this article, we present a differential multi‐omics model approach, including metabolomics, genomics, and bioinformatic models, to distinguish normal glands from thyroid tumours. Additionally, we are proposing biomarkers that could indicate potential metastatic diseases in papillary thyroid cancer (PTC), a sub‐class of DTC.ResultsNormal and tumour thyroid tissue from DTC patients had a distinct yet well‐defined metabolic profile with high levels of anabolic metabolites and/or other metabolites associated with the energy maintenance of tumour cells. The consistency of the DTC metabolic profile allowed us to build a bioinformatic classification model capable of clearly distinguishing normal from tumor thyroid tissues, which might help diagnose thyroid cancer. Moreover, based on PTC patient samples, our data suggest that elevated nuclear and mitochondrial DNA mutational burden, intra‐tumour heterogeneity, shortened telomere length, and altered metabolic profile reflect the potential for metastatic disease.DiscussionAltogether, this work indicates that a differential and integrated multi‐omics approach might improve DTC management, perhaps preventing unnecessary thyroid gland removal and/or radioiodine therapy.ConclusionsWell‐designed, prospective translational clinical trials will ultimately show the value of this integrated multi‐omics approach and early diagnosis of DTC and potential metastatic PTC.
Rectovaginal endometriosis with nodular smooth muscle metaplasia diagnosed via transrectal ultrasound‐guided fine‐needle aspiration cytology: An underused minimally invasive diagnostic technique?
Authors: Molina Mariel , Moreno Gustavo A. , Singh Reena , Hunt Bryan , Giorgadze Tamar A. 
Published: June 2023

AbstractRectovaginal endometriosis is a severe variant of deeply infiltrating endometriosis. Laparoscopic assessment with tissue sampling remains the gold standard for diagnosis of endometriosis. However, transvaginal (TVUS) and transrectal ultrasound (TRUS) have been shown to be especially helpful in the diagnosis of deep endometriosis. We present the case of a 49‐year‐old female with menorrhagia, dysmenorrhea, and constipation. Upon pelvic examination, an incidental mass was palpated. A computed tomography (CT) scan revealed an anterior rectal wall mass and colonoscopy was non‐diagnostic. Further work‐up with MRI showed a 3.9 cm mass centered within the upper rectovaginal septum. TRUS guided fine‐needle aspiration (TRUS‐FNA) revealed cohesive epithelial cell groups without significant cytologic atypia and a second population of bland spindle cells. Cell block slides showed glandular epithelium with associated stroma that exhibited endometrial morphology and immunophenotype. Nodular fragments of spindle cells with smooth muscle immunophenotype and fibrosis were also present. The overall morphologic findings were consistent with rectovaginal endometriosis with nodular smooth muscle metaplasia. Medical management with nonsteroidal aromatase inhibitor with radiologic follow‐up was selected. Rectovaginal endometriosis represents a type of deep endometriosis usually associated with severe pelvic symptoms. Metaplastic smooth muscle cells are a frequent component of endometriosis in the rectovaginal pouch with nodular growth and may present diagnostic challenges. TRUS‐FNA is a minimally invasive procedure that can provide an accurate diagnosis of endometriosis, even in this variant of deep infiltrating disease.
Computational fluid dynamic analysis of hydrogen‐injected natural gas for mixing and transportation behaviors in pipeline structures
Authors: Yan Shuangjie , Jia Guanwei , Xu Weiqing , Li Rui , Lu YangHui , Cai Maolin 
Published: June 2023

AbstractThe transportation of hydrogen is a weak link in the large‐scale development of the hydrogen energy industry. Injecting hydrogen into the natural gas pipeline network for transportation is an efficient way to achieve the large‐scale, long‐distance, and low‐cost transportation of hydrogen. Hydrogen can lead to hydrogen embrittlement in natural gas pipelines and cause safety incidents if hydrogen and natural gas are not mixed uniformly. Therefore, it is necessary to study the blending process and blending uniformity of hydrogen and natural gas. In this study, a three‐dimensional model of the hydrogen‐injected natural gas pipeline was constructed. The effects of hydrogen injection inlet and turbulator configuration on the mixing process of hydrogen and natural gas were investigated using a computational fluid dynamics approach. The results show that increasing the number of hydrogen injection inlets shortens the distance L98% of uniform mixing of hydrogen and natural gas. Increasing the radial distance r from the initial hydrogen mixing positions to the center of the pipeline will shorten the distance for uniform gas mixing in the pipeline. The addition of turbulator configurations in the pipeline significantly reduces the distance to uniform gas mixing. Changing the distance Lturb from the turbulator to the initial mixing position further shortens the distance between hydrogen and natural gas mixing uniformly. The results of this study provide a reference for the structural design of the hydrogen–natural gas mixing pipeline and the gas distribution state during the mixing process.
Waste heat recovery of the hydrogen–water mixture from high‐temperature water electrolysis by cascade heat pump for steam generation
Authors: Fang Zhicong , Chen Zhiguang , Yang Zekun , Zhang Shuhao 
Published: June 2023

AbstractWaste heat recovery is common in high‐temperature water electrolysis production, but the latent heat from the hydrogen–water mixture is not fully utilized, and the electric heater to generate steam consumes much energy. In this research, a cascade heat pump is proposed to recover the latent heat from electrolysis products and generate steam. The heat pump can save as much as 65% electric energy compared with a single electric heater. Although in some cases, the latent heat is not enough to generate sufficient steam, it can still save 46.1% of energy. Also, this research emphasizes the significant influence of hydrogen and water proportion in electrolysis products. Compared with 80% water proportion, 50% water proportion can save 1.67 MW energy just in the water vaporization process for a 10 MW electrolyzer. The payback period is 3.43 years, which makes it worth investing.
Exploring magnetic capture to improve the detection of human norovirus in strawberries
Authors: Bai Yalong , Pu Chunmin , Suo Yujuan , Zhang Xufu , Qu Yang , Feng Bo , Huang Liujuan , Shao Yi , Dai Yingchun 
Published: June 2023

AbstractHuman norovirus is a leading cause of foodborne illness worldwide, and its detection in foods remains a challenge due to the difficulty of eluting and enriching trace amounts of viral particles. In this study, we developed porcine gastric mucin–labeled magnetic capture probes and optimized the conditions for their binding with norovirus, elution of viral particles, and RNA isolation based on the previous research. We evaluated the magnetic capture‐based pretreatment method in artificially contaminated strawberry samples and compared it with the conventional polyethylene glycol precipitation–based method using reverse transcriptional‐quantitative PCR (RT‐qPCR). The results revealed that Tris·HCl (pH 9.5) was the optimal buffer for the binding of magnetic probes with viral particles, and RNA recovery rate obtained by heating‐based release was significantly higher than that obtained by a commercial kit, with only active virus being captured. This buffer was similar to the RT‐qPCR buffer, which facilitated the sensitivity of RT‐qPCR. Furthermore, the buffer was an effective solution for eluting viral particles from foods in previous research and was optimized to ensure maximum elution and no inhibitory effect on viral capture. We explored multiple factors or steps to ensure that the sensitivity of our method was three orders of magnitude higher than that of the conventional method. Our findings suggest that magnetic capture‐based detection has great potential for the sensitive detection of human norovirus.
An investigation on the prevalence of microplastic in commercial and open pan salts obtained from Cox's Bazar and Maheshkhali region of Bay of Bengal (Bangladesh)
Authors: Mazumder Debapriya , Quader Md. Fahad Bin , Saha Suvanker , Islam Md. Ashraful , Sarker Rakha Hari , Chowdhury Arpan Mitra 
Published: June 2023

AbstractUnrestrained utilization of plastic has reached an intemperate state, menacing environment and human lives. The preliminary focus of this research was to investigate and divulge the contemporary status of microplastics (MPs) in commercialized and open pan salts from Cox's Bazar and Maheshkhali channels. A total of 27 samples were obtained. The samples were analyzed for the prevalence of MPs by FTIR and Raman spectroscopy (RS); the prevailing amount, color, size, and shapes were analyzed by stereomicroscope and SEM. The abundance of high‐density polyethylene, polyethylene terephthalate (PET), and low‐density polyethylene (LDPE) were detected by FTIR, meanwhile exuberance of cellulose acetate, polypropylene, PET, LDPE, and Nylon 6 were identified by RS. The average quantifications of MPs in Cox's Bazar, Maheshkhali, and packaged salts were found to be 6851.11 ± 538.18, 5638.89 ± 1001.18, and 3405.56 ± 638.57 per kg, respectively. ANOVA resulted in highly significant association between MPs and sampling sites (p = .001*). Post hoc Tukey's test revealed prominent link between commercialized and open pan salts based on the amount of MPs (p = .001*). The most prevalent colors were purple (28%) and blue (27%). The most frequent shapes were fibrous (79%) and fragmented (19.9%) MPs. The smallest MP was detected in commercial salt (1.55 μm), nearly identical and closer to the size of nanoplastics.