bluuebunny/crossref_metadata_2025_split huggingface dataset:
Cuproptosis-related genes signature and validation of differential expression and the potential targeting drugs in temporal lobe epilepsy
Authors: Yang Xiaolin , Zhang Xiaoqing , Shen Kaifeng , Wang Zhongke , Liu Guolong , Huang Kaixuan , He Zeng , Li Yang , Hou Zhi , Lv Shengqing , Zhang Chunqing , Yang Hui , Liu Shiyong , Ke Yanyan 
Published: June 2023

Introduction: Temporal lobe epilepsy (TLE) is the most common subtype of epilepsy in adults and is characterized by neuronal loss, gliosis, and sprouting mossy fibers in the hippocampus. But the mechanism underlying neuronal loss has not been fully elucidated. A new programmed cell death, cuproptosis, has recently been discovered; however, its role in TLE is not clear.Methods: We first investigated the copper ion concentration in the hippocampus tissue. Then, using the Sample dataset and E-MTAB-3123 dataset, we analyzed the features of 12 cuproptosis-related genes in TLEs and controls using the bioinformatics tools. Then, the expression of the key cuproptosis genes were confirmed using real-time PCR and immunohistochemical staining (IHC). Finally, the Enrichr database was used to screen the small molecules and drugs targeting key cuproptosis genes in TLE.Results: The Sample dataset displayed four differentially expressed cuproptosis-related genes (DECRGs; LIPT1, GLS, PDHA1, and CDKN2A) while the E-MTAB-3123 dataset revealed seven DECRGs (LIPT1, DLD, FDX1, GLS, PDHB, PDHA1, and DLAT). Remarkably, only LIPT1 was uniformly upregulated in both datasets. Additionally, these DECRGs are implicated in the TCA cycle and pyruvate metabolism—both crucial for cell cuproptosis—as well as various immune cell infiltrations, especially macrophages and T cells, in the TLE hippocampus. Interestingly, DECRGs were linked to most infiltrating immune cells during TLE’s acute phase, but this association considerably weakened in the latent phase. In the chronic phase, DECRGs were connected with several T-cell subclasses. Moreover, LIPT1, FDX1, DLD, and PDHB were related to TLE identification. PCR and IHC further confirmed LIPT1 and FDX1’s upregulation in TLE compared to controls. Finally, using the Enrichr database, we found that chlorzoxazone and piperlongumine inhibited cell cuproptosis by targeting LIPT1, FDX1, DLD, and PDHB.Conclusion: Our findings suggest that cuproptosis is directly related to TLE. The signature of cuproptosis-related genes presents new clues for exploring the roles of neuronal death in TLE. Furthermore, LIPT1 and FDX1 appear as potential targets of neuronal cuproptosis for controlling TLE’s seizures and progression.
Identification of signaling pathways modifying human dopaminergic neuron development using a pluripotent stem cell-based high-throughput screening automated system: purinergic pathways as a proof-of-principle
Authors: Boissart Claire , Lasbareilles Marie , Tournois Johana , Chatrousse Laure , Poullion Thifaine , Benchoua Alexandra 
Published: June 2023

Introduction: Alteration in the development, maturation, and projection of dopaminergic neurons has been proposed to be associated with several neurological and psychiatric disorders. Therefore, understanding the signals modulating the genesis of human dopaminergic neurons is crucial to elucidate disease etiology and develop effective countermeasures.Methods: In this study, we developed a screening model using human pluripotent stem cells to identify the modulators of dopaminergic neuron genesis. We set up a differentiation protocol to obtained floorplate midbrain progenitors competent to produce dopaminergic neurons and seeded them in a 384-well screening plate in a fully automated manner.Results and Discussion: These progenitors were treated with a collection of small molecules to identify the compounds increasing dopaminergic neuron production. As a proof-of-principle, we screened a library of compounds targeting purine- and adenosine-dependent pathways and identified an adenosine receptor 3 agonist as a candidate molecule to increase dopaminergic neuron production under physiological conditions and in cells invalidated for the HPRT1 gene. This screening model can provide important insights into the etiology of various diseases affecting the dopaminergic circuit development and plasticity and be used to identify therapeutic molecules for these diseases.
Tensiomyography: from muscle assessment to talent identification tool
Authors: Čular Dražen , Babić Matej , Zubac Damir , Kezić Ana , Macan Iva , Peyré-Tartaruga Leonardo Alexandre , Ceccarini Francesco , Padulo Johnny 
Published: June 2023

Introduction: Tensiomyography (TMG) is a non-invasive and cost-effective tool that is gaining popularity in fields such as sports science, physical therapy, and medicine. In this narrative review, we examine the different applications of TMG and its strengths and limitations, including its use as a tool for sport talent identification and development.Methods: In the course of crafting this narrative review, an exhaustive literature search was carried out. Our exploration spanned several renowned scientific databases, such as PubMed, Scopus, Web of Science, and ResearchGate. The materials we sourced for our review included a broad spectrum of both experimental and non-experimental articles, all focusing on TMG. The experimental articles featured varied research designs including randomized controlled trials, quasi-experiments, as well as pre-post studies. As for the non-experimental articles, they encompassed a mix of case-control, cross-sectional, and cohort studies. Importantly, all articles included in our review were written in English and had been published in peer-reviewed journals. The assortment of studies considered provided a holistic view of the existing body of knowledge on TMG, and formed the basis of our comprehensive narrative review.Results: A total of 34 studies were included in the review, organized into three sections: 1) assessing muscle contractile properties of young athletes, 2) using TMG in the talent identification and development process and 3) Future research and perspectives. According to data presented here, the most consistent TMG parameters for determining muscle contractile properties are radial muscle belly displacement, contraction time, and delay time. Biopsy findings from the vastus lateralis (VL) confirmed TMG as a valid tool for estimating the ratio of myosin heavy chain (%MHC-I).Conclusion: TMGs ability to estimate the ratio of %MHC-I has the potential to aid in the selection of athletes with the muscle characteristics best suited for a particular sport, eliminating the need for more invasive procedures. However, more research is warranted to fully understand TMG’s potential and its reliability when used with young athletes. Importantly, the use of TMG technology in this process can positively impact health status, reducing the frequency and severity of injuries and the duration of recovery, and subsequently can reduce drop out rates among youth athletes. Future studies should look at twin youth athletes, as a model capable of discriminating between the influence of hereditary factors vs. environmental factors, in therms of muscle contractility and TMG’s potential for instance.
Long-term liver lesion tracking in contrast-enhanced ultrasound videos via a siamese network with temporal motion attention
Authors: Tian Haozhe , Cai Wenjia , Ding Wenzhen , Liang Ping , Yu Jie , Huang Qinghua 
Published: June 2023

Propose: Contrast-enhanced ultrasound has shown great promises for diagnosis and monitoring in a wide range of clinical conditions. Meanwhile, to obtain accurate and effective location of lesion in contrast-enhanced ultrasound videos is the basis for subsequent diagnosis and qualitative treatment, which is a challenging task nowadays.Methods: We propose to upgrade a siamese architecture-based neural network for robust and accurate landmark tracking in contrast-enhanced ultrasound videos. Due to few researches on it, the general inherent assumptions of the constant position model and the missing motion model remain unaddressed limitations. In our proposed model, we overcome these limitations by introducing two modules into the original architecture. We use a temporal motion attention based on Lucas Kanade optic flow and Karman filter to model the regular movement and better instruct location prediction. Moreover, we design a pipeline of template update to ensure timely adaptation to feature changes.Results: Eventually, the whole framework was performed on our collected datasets. It has achieved the average mean IoU values of 86.43% on 33 labeled videos with a total of 37,549 frames. In terms of tracking stability, our model has smaller TE of 19.2 pixels and RMSE of 27.6 with the FPS of 8.36 ± 3.23 compared to other classical tracking models.Conclusion: We designed and implemented a pipeline for tracking focal areas in contrast-enhanced ultrasound videos, which takes the siamese network as the backbone and uses optical flow and Kalman filter algorithm to provide position prior information. It turns out that these two additional modules are helpful for the analysis of CEUS videos. We hope that our work can provide an idea for the analysis of CEUS videos.
How do people experience innocent suffering?
Authors: Smirnov Evgeny 
Published: June 2023

IntroductionThe paper examines the psychological facet of innocent suffering. One can find a description of this phenomenon in social psychology as a factor that affects the belief in a just world, but there is a lack of qualitative scientific data about related psychological features, processes, copings, and consequences on the personality level.MethodsTo study innocent suffering, semi-structured in-depth interview was conducted (31 respondents, ~223 minutes per respondent, 6,924 min in total) aimed to gather data about the experiences of innocent sufferings happened to participants. For the analysis of texts, a narrative and content analysis are used within the framework of grounded theory. The reliability of the results is based on expert assessment.Results and discussionAs a result, six essential properties of innocent sufferings were identified: complexity, stability, distress, injustice, casual incoherence, and breaks of integrity of a life story. The most “popular” life domains, in which participants reported about innocent sufferings, are violence, abuse (physical and psychological), and quitting romantic relationships. It is proposed a scientific definition of innocent suffering and the prototype of the phenomenon.
Effects of the COVID-19 pandemic on Czech citizens: how do depression and anxiety symptoms influence cognitive, behavioral, and emotional changes?
Authors: Hajkova Dagmar , Sandora Jan , Žídková Radka , Malinakova Klara , Novak Lukas 
Published: June 2023

BackgroundThis study examined the impact of anxiety and depression symptoms during the first stage of the COVID-19 pandemic on the behavioral, cognitive, and emotional changes of the Czech population.MethodsThe research sample (n = 2363; 48.83 ± 16.53 years; 50.15% men) was obtained using an online survey. Depression and anxiety symptoms were measured using the Overall Depression Severity and Impairment Scale (ODSIS) and the Overall Anxiety Severity and Impairment Scale (OASIS) and associations were adjusted for age, gender, and economic status.ResultsThe results showed that increased symptoms of anxiety and depression were significantly linked to feelings of loneliness, helplessness, reduced quality of relationship with a partner, higher probabilities of alcohol abuse, food consumption, and contemplation of existential questions. Higher symptoms of anxiety were associated with feelings of being threatened. Higher symptoms of depression symptoms increased tobacco abuse.ConclusionDuring the first stage of the COVID-19 pandemic, higher symptoms of anxiety and depression among Czech citizens were associated with behavioral, cognitive, and emotional changes.
The role of chess in the development of children-parents’ perspectives
Authors: Nanu Costica Ciprian , Coman Claudiu , Bularca Maria Cristina , Mesesan-Schmitz Luiza , Gotea Mihaela , Atudorei Ioana , Turcu Ioan , Negrila Ion 
Published: June 2023

IntroductionThe study examines the role of chess in the development of children from the perspectives of parents. The research focused on analyzing the parents’ perceptions about chess’s role in their children’s development, on finding out how the perception of parents differs depending on whether they know how to play chess or not, and on outlining the profile of the parents whose children play chess.The study was conducted in Romania.MethodsIn order to conduct the study, a quantitative research method was used, while having as a research instrument a non-standardized questionnaire. The questionnaire was applied to parents of chess-playing children who are members of chess clubs from Romania. The sample of the study comprises 774 respondents.ResultsThe results of our research showed that parents are of the opinion that chess helps children develop their cognitive abilities, their character and their competitive spirit. Most of the parents focused on highlighting the positive effects of chess on the development of their children. Parents also considered that chess helped their children develop positive emotions and helped them overcome negative emotions. The results revealed differences between the opinions of parents depending on whether they know how to play chess or not. Thus, parents who do know how to play chess were more likely to focus on the positive effects of the game on the development of their children, and those who know how to play chess were also more satisfied with their children’s accumulated knowledge following chess lessons.DiscussionFindings extend our understanding of how parents perceive the way chess influences the development of their children, it offered us a perspective on the perceived benefits of chess, benefits which should be further analyzed in order to identify under what circumstances chess could be introduced in the school curriculum.
Perceptions of medical students and their facilitators on clinical communication skills teaching, learning, and assessment
Authors: Dewi Sari Puspa , Wilson Amanda , Duvivier Robbert , Kelly Brian , Gilligan Conor 
Published: June 2023

IntroductionDespite various efforts to develop communication skills (CS) in the classroom, the transfer of these skills into clinical practice is not guaranteed. This study aimed to identify barriers and facilitators of transferring CS from the classroom to clinical environments.MethodsA qualitative study was conducted at one Australian medical school to explore the experiences and perceptions of facilitators and students in relation to teaching and learning clinical CS. Thematic analysis was used to analyze data.ResultsTwelve facilitators and sixteen medical students participated in semi-structured interviews and focus-group discussions, respectively. Primary themes included the value of teaching and learning, alignment between approaches to teaching and actual clinical practices and students’ perceptions of practice, and challenges in different learning environments.DiscussionThis study reinforces the value of teaching and learning CS by facilitators and students. Classroom learning provides students with a structure to use in communicating with real patients, which can be modified to suit various situations. Students have limited opportunities, however, to be observed and receive feedback on their real-patient encounters. Classroom session that discussed CS experiences during clinical rotation is recommended to strengthen learning both the content and process of CS as well as transitioning to the clinical environment.
An Inversion Method for Geoacoustic Parameters in Shallow Water Based on Bottom Reflection Signals
Authors: Wang Zhuo , Ma Yuxuan , Kan Guangming , Liu Baohua , Zhou Xinghua , Zhang Xiaobo 
Published: June 2023

The inversion method based on the reflection loss-grazing angle curve is an effective tool to obtain local underwater acoustic parameters. Because geoacoustic parameters vary in sensitivity to grazing angle, it is difficult to get accurate results in geoacoustic parameter inversion based on small-grazing-angle data in shallow water. In addition, the normal-mode model commonly used in geoacoustic parameter inversion fails to meet the needs of accurate local sound field simulation as the influence of the secant integral is ignored. To solve these problems, an acoustic data acquisition scheme was rationally designed based on a sparker source, a fixed vertical array, and ship drifting with the swell, which could balance the trade-off among signal transmission efficiency and signal stability, and the actual local acoustic data at low-to-mid frequencies were acquired at wide grazing angles in the South Yellow Sea area. Furthermore, the bottom reflection coefficients (bottom reflection losses) corresponding to different grazing angles were calculated based on the wavenumber integration method. The local seafloor sediment parameters were then estimated using the genetic algorithm and the bottom reflection loss curve with wide grazing angles, obtaining more accurate local acoustic information. The seafloor acoustic velocity inverted is cp=1659 m/s and the sound attenuation is αp=0.656 dB/λ in the South Yellow Sea. Relevant experimental results indicate that the method described in this study is feasible for local inversion of geoacoustic parameters for seafloor sediments. Compared with conventional large-scale inversion methods, in areas where there are significant changes in the seabed sediment level, this method can obtain more accurate local acoustic features within small-scale areas.
The Effect of Elective Ligation of the Arteriovenous Fistula on Cardiac and Renal Functions in Kidney Transplant Recipients
Authors: Masson Grégoire , Viva Tommaso , Huart Justine , Weekers Laurent , Bonvoisin Catherine , Bouquegneau Antoine , Maweja Sylvie , Hamoir Etienne , Seidel Laurence , Pottel Hans , Lancellotti Patrizio , Jouret François 
Published: June 2023

Key Points

Surgical AVF ligation in KTRs is associated with a significant increase in diastolic BP while systolic BP remains stable.AVF closure in KTRs leads to an improvement of LV and LA morphology and a decrease in serum NT-proBNP levels.There is no significant effect of AVF ligation on kidney allograft function: The eGFR remains stable over time.

Background
Kidney transplantation is considered as the best kidney replacement therapy, and arteriovenous fistula (AVF) is the preferred vascular access for hemodialysis. The systematic ligation of a functioning AVF in stable kidney transplant recipients (KTRs) remains debatable.

Methods
In this prospective study, we investigated the hemodynamic effect of the surgical closure of AVF in KTRs. Forty-three KTRs underwent an ambulatory BP monitoring before surgical closure of AVF (T0) and 12 months later (M12), as well as measurement of serum cardiac biomarkers (i.e., soluble suppression of tumorigenicity 2, N-terminal pro b-type natriuretic peptide [NT-proBNP], and galectin-3). Serum tests were also performed 6 months after AVF closure (M6). An echocardiographic examination was performed at each time point. All serum creatinine values were collected to compare the individual eGFR slopes before versus after AVF closure. The latest measure of the AVF flow before kidney transplantation was recorded.

Results
Diastolic BP significantly rose from T0 to M12: +4.4±7.3 mm Hg (P = 0.0003) for 24h, +3.8±7.4 mm Hg (P = 0.0018) during the day, and +6.3±9.9 mm Hg (P = 0.0002) during the night, leading to an increased proportion of KTRs with European Society of Hypertension (ESH)-defined arterial hypertension after AVF ligation. No change was observed for systolic BP. NT-proBNP significantly dropped between T0 and M6 (345 [190; 553] to 230 [118; 458] pg/ml, P = 0.0001) and then remained stable from M6 to M12 while suppression of tumorigenicity 2 and galectin-3 levels did not change from T0 to M12. We observed a significant decrease in left ventricular (LV) end-diastolic volume, LV end-systolic volume, LV mass, interventricular septum diameter, left atrial volume, and tricuspid annular plane systolic excursion from T0 to M6 and then a stability from M6 to M12. LV ejection fraction and eGFR slope remained stable during the whole study. These observations remained unchanged after adjustment for AVF flow.

Conclusion
The closure of a patent AVF in KTRs is associated with elevation of diastolic BP, drop in serum NT-proBNP levels, reduction of left ventricular and atrial dimensions, and stability of eGFR slope.

Podcast
This article contains a podcast at https://dts.podtrac.com/redirect.mp3/www.asn-online.org/media/podcast/K360/2023_12_01_KID0000000000000198.mp3
Komunikasi Interpersonal Orang Tua Bekerja dan Anak Sekolah Dasar Pada Pembelajaran Daring Selama Pandemi Covid-19
Authors: Amalia Sarah 
Published: June 2023

Saat Pandemi Covid-19 pertengahan bulan Maret 2020 lalu pemerintah mengeluarkan kebijakan didalam dunia pendidikan dengan memberlakuan pembelajaran daring pada seluruh tingkatan pendidikan guna memutus rantai penyebaran virus. Kendala komunikasi yang kurang berjalan dengan baik menjadi kendala utama yang dirasakan. Penelitian ini bertujuan untuk mengetahui bagaimana proses komunikasi interpersonal orang tua bekerja dan anak sekolah dasar yang terjadi pada proses pembelajaran daring. Penelitian ini merupakan penelitian kualitatif deskriptif dengan paradigma konstruktivisme. Pengumpulan data menggunakan observasi, wawancara mendalam, dan dokumentasi. Teknik analisis data analisis dari model Miles dan Huberma dengan teknik keabsahan data yaitu triangulasi sumber. Teori yang digunakan ialah teori komunikasi interpersonal DeVito dengan kelima ciri keterbukaan, empati, dukungan, rasa positif, dan kesetaraan yang menjelaskan komunikasi yang dilakukan oleh orang tua dan anak yang telah memiliki hubungan dekat satu sama lain akan menunjang proses pembelajaran.
Hasil dapat disimpulkan bahwa secara keseluruhan ditinjau dari kelima ciri komunikasi interpersonal menunjukan bahwa orang tua bekerja dan anak mampu menjalin komunikasi yang baik dan mampu mengatasi kendala serta kesulitan yang dihadapi oleh keduanya pada saat pembelajaran daring sehingga saat melakukan pembelajaran daring dapat berjalan secara optimal
Pengaruh Kampanye #PawaiBebasPlastik2021 Terhadap Perilaku Peduli Lingkungan Followers Instagram @iddkp
Authors: Hanifah Wanda , Prananingrum Elisabeth Nugrahaeni , Sutjipto Vera Wijayanti 
Published: June 2023

ABSTRACT
Since the Covid-19 pandemic, plastic problems have increased, because they are triggered by changes in behavior and consumption in the community. Environmental care behavior becomes a measuring tool to determine human behavior about the environment. Public awareness of the urgency of the issue of environmental pollution due to plastic waste is also growing, it can be seen from the emergence of various campaigns in Indonesia, thus this study aims to determine the effect of the #PawaiBebasPlastik2021 campaign on the environmental care behavior of Instagram followers iddk. This type of research is quantitative with a survey method. The number of respondents as many as 99. The results of this study indicate that the value of the correlation coefficient test is in the interval 0.61 – 0.80, this value indicates that the #PawaiBebasPlastik2021 campaign has a strong influence on the environmental care behavior of Instagram followers @Iddkp and is positive. Furthermore, based on the value of the results of the determination test, it is known that the coefficient of determination is 43.2% which shows that 43.2% of caring behavior is influenced by the campaign, but the remaining 56.8% is influenced by other factors.
Keywords: Social Media, Campaigns, Plastic Waste, Caring Behavior