bluuebunny/crossref_metadata_2025_split huggingface dataset:
Queen loss unmasks cryptic worker influence and decentralizes the bumble bee social network
Authors: Ruttenberg Dee M. , Wolf Scott W. , Webb Andrew E. , Wyman Eli S. , White Michelle L. , Melo Diogo , Traniello Ian M. , Kocher Sarah D. 
Published: January 2025

AbstractBumble bees (Bombus impatiens) undergo a radical transformation in social dynamics as a natural part of their colony life cycle. Early in the season, bumble bee societies are eusocial and contain a reproductive queen and functionally sterile workers. As the colony grows, the colony transitions to a competition phase in which reproductive division of labor breaks down and some workers activate their ovaries and lay unfertilized, male-destined eggs. This transition represents a key shift in colony organization, but its underlying social dynamics, including the relationship between individual- and colony-level behavior, are poorly understood. Using multianimal pose tracking to quantify social interactivity and related movement dynamics, we compared queenright and queenless colonies that capture these distinct cooperative and competitive phases of the colony life cycle. Queenright colonies displayed highly centralized social networks in which the queen received, rather than initiated, the majority of social interactions. In contrast, queenless colonies displayed higher worker interaction rates, and a subset of workers exhibited queen-like social behaviors and higher reproductive potential relative to nestmates. The unmasking of these “influencers” in a queenless, competitive context transformed the colony’s social network from a centralized to distributed social organization with higher overall network efficiency. Thus, the bumble bee queen masks a hidden relationship between worker influence and reproductive potential that underlies their latent social flexibility. In the absence of a queen, the colony social structure is transformed, revealing that significant modifications in information transfer can accompany shifts in colony social organization and highlighting a novel layer of resilience in this social system.
Antiproliferative Activity, Phytochemistry, Network Pharmacology, Molecular Docking and Gene Expression Analysis ofMaerua edulisExtracts against Human Cervical Cancer Cell Line
Authors: Lagu Inyani John Lino , Letoluo Rakita , Kamau Sally Wambui , Kuria James Menni , Musila Fredrick Mutie , Kungurtsev Vyacheslav , Nyamai Dorothy W. , Njeru Sospeter Ngoci 
Published: January 2025

AbstractMaerua edulisexhibits significant antiproliferative activity against HeLa cells, with hexane and ethyl acetate extracts showing IC50values of 0.02% and 47.42µg/mL, respectively. Gas Chromatography-Mass Spectrometry (GC-MS) analysis identified key phytochemicals such as diisooctyl phthalate, squalene, and stigmasta-3,5-diene, which were associated with the regulation of apoptotic and cell cycle-related genes (BCL2, CDK2, TP53). Gene expression assays confirmed the modulation of these targets, suggesting the therapeutic potential ofM. edulisin cervical cancer. Further in vivo studies are recommended to validate these findings and establish its safety profile.
Ecological niche stability ofBiomphalariaintermediate hosts forSchistosoma mansoniunder extreme flooding and seasonal change
Authors: Iacovidou Melissa A. , Byaruhanga Anatol M. , Besigye Fred , Nabatte Betty , Kabatereine Narcis B. , Chami Goylette F. 
Published: January 2025

AbstractUnderstanding spatiotemporal distributions and niches of vectors and intermedi-ate hosts for ecologically dependent pathogens is crucial for identifying endemic areas, assessing habitat suitability for transmission, and targeting interventions for both the environment and humans. This study focuses onBiomphalaria sudan-icaandB. stanleyi, intermediate hosts of intestinal schistosomes, with over 700 million people at risk in endemic areas. We identified how extreme flooding and seasonal changes influence habitat preferences and species interactions across 674 sites in 52 villages in rural Uganda between 2022–2024. A comprehensive analy-sis of ground truth data was conducted, covering spatiotemporal information, site characteristics, physicochemical parameters, ecological factors, and human activi-ties. Spatiotemporal models incorporating a new polygon-based method to account for space, bypassing limitations of administrative boundaries, with time as a fixed effect were developed to analyse snail abundance.B. sudanicawas associated with marshy sites near lake shorelines and presence of hyacinths, whileB. stanleyiwas more likely found in deeper waters withVallisneriaplants. However, both species often cohabited at the same sites. The extent of habitat suitability for each species fluctuated seasonally and more starkly with extreme flooding resulting in switch-ing of dominance between species. Our study shows that climatic variations may influence local changes in habitat suitability without necessitating an expansion of environmental areas. By providing a robust, generalisable spatiotemporal mod-elling pipeline, our study enables precise tracking of dynamic ecological niches in a changing climate that, if replicated in other areas, can be used to better target environmental and human interventions.
Integration of differential expression under drought with gene family expansion unique to drought tolerant species predicts candidate genes for drought adaptation in Brassicaceae species
Authors: Uebermuth-Feldhaus Carolin , Schoof Heiko 
Published: January 2025

AbstractBackgroundIn order to predict candidate genes for drought adaptation, we analyze genomic data of the more drought resistant Brassicaceae speciesEutrema salsugineumandArabidopsis lyratacompared to more drought sensitive speciesArabidopsis thalianaandBrassica napus.We combine gene family expansion, which is an important driver of evolution in plants, unique to the drought resistant species with differential expression under drought (DE).ResultsWe show that combining trait-specific gene family expansion with differential expression predicts a concise set of candidate genes. To demonstrate that these are relevant for drought adaptation in tolerant species we show enrichment of DE conserved between both tolerant species, DE unique to the tolerant species, and up regulation. We show that specific functions are enriched, and that the set contains genes with functions such as root development in line with drought adaptation based on evidence from other species, while the background of all differentially expressed genes (DEGs) contains many general stress reaction genes. Whereas DEGs in general are rarely under diversifying selection, signatures of diversifying selection are slightly enriched in the candidate gene families, highly significantly enriched in DEGs in tolerant species-specific expanded gene families, and in contrast not enriched in DEGs in sensitive species-specific expanded gene families.ConclusionsOur approach identifies a concise and functionally relevant set of candidate genes for drought adaptation with promising targets for functional studies and crop improvement for drought tolerance. We propose that our method can also be used to predict candidate genes for adaptation to other environmental factors.
Androgen receptor inhibition extends PARP inhibitor activity in prostate cancer models beyond BRCA mutations and defects in homologous recombination repair
Authors: Illuzzi Giuditta , Galbiati Alessandro , Staniszewska Anna D. , Hanson Robert , Michaloglou Chrysiis , Cooke Sophie , Uznańska Karolina , Białecka Maja , Solarczyk Kamil , Dev Harveer , Massie Charles E. , Albertella Mark , Leo Elisabetta , Forment Josep V. , O’Connor Mark J. 
Published: January 2025

AbstractRecent phase 3 clinical trial readouts have shown benefit of the combination of poly(ADP-ribose) polymerase inhibitors (PARPi) with androgen receptor (AR) pathway inhibitors (ARPi) in metastatic, castration-resistant prostate cancer (mCRPC). While benefit was particularly evident in patients with tumours harbouring mutations in homologous recombination repair (HRR) genes, improved outcomes were also observed in patients with no such defined alterations in their cancers. Although there is literature linking AR activity with DNA repair pathways, the basis of the interaction between the AR and PARP is unclear. Here, we show that benefit of the combination of ARPi and PARPi in prostate cancer in vitro and in vivo models with no HRR mutations requires ARPi-responsive cells and a PARPi with PARP1-trapping activity, and does not involve an effect of PARPi treatment in modulating the transcriptional role of the AR. Combination benefit is driven by an increase in DNA damage in the form of DNA double-strand breaks and micronuclei formation, which is not due to a direct control of HRR gene transcription by the AR. In addition, we uncover a novel role of PARP1 in modulating AR recruitment to chromatin in the presence of DNA damage. These data shed new light on the interplay between PARP1 and the AR in dealing with genotoxic insults and provide a mechanism of action consistent with the observed clinical benefit of the combination of PARPi and ARPi in patients with prostate cancer.Statement of significanceCombination of androgen receptor pathway inhibitors and PARP inhibitors has shown efficacy in prostate cancer. We provide a mechanistic explanation through increased DNA damage accumulation observed in combination vs single-agent treatments.
Gut meta-virome reveals potential zoonotic pathogens and environmental RNA viruses carried by non-human primates
Authors: Yan Yujie , Li Yuhang , Yang Linshan , Wu Haojie , Wu Fan , Chang Hongli , Hu Zhengfeng , He Shujun , Ren Yi , Zhu Lifeng , Li Baoguo , Guo Songtao 
Published: January 2025

AbstractThe majority of human infectious diseases originate from mammals and are inherently zoonotic. Non-human primates (NHPs) are not only carriers of many zoonotic pathogens, but also the best intermediary for virus shift from harmless to harmful due to their similar phylogenetic relationship with humans. Knowledge of NHP viral composition and its underlying information can therefore provide an assessment of the risk of cross-species transmission and spillover of zoonotic diseases. However, studies that have successfully eliminated the effects of different natural habitat environments on viral carriage have been limited, making it difficult to identify which zoonotic viruses and potentially species-barrier-crossing viruses are carried by widely distributed NHP hosts. Here, we analyze viruses excreted in feces from NHP hosts and reveal the presence of possible zoonotic pathogens. Most of the viruses found come from plants that are eaten by them. Analysis of the evolutionary relationships of potential pathogens suggests that other clinical isolates are genetically correlated to HIV, PBV, and EV we identified. This study provides foundational data for surveillance of NHP enteroviruses that could help to be challenging to track potential human viruses carried by NHP in the absence of clinical surveillance.ImportanceAs a result of internationalization and other factors, many viruses have broken through the limits of geographical units and spread into human society, causing many emerging and re-emerging infectious disease hazards. However, the virus spreads and is by no means infectious to humans overnight. There is a phenomenon of host shift, in which the virus does not necessarily cause disease in an intermediate host, but causes significant damage when transmitted to humans. We focus on captive non-human primates, close relatives of humans in cities, because the phenomenon of host shifts is related to both biological similarity and geographical proximity of the host. Primates may carry zoonotic viruses and viruses that are currently incapable of infecting humans but have evolved with pathogenic potential. In summary, comprehensive viral surveillance and screening of captive nonhuman primates is necessary.
Fis1 is required for the development of the dendritic mitochondrial network in pyramidal cortical neurons
Authors: Strucinska Klaudia , Kneis Parker , Pennington Travis , Cizio Katarzyna , Szybowska Patrycja , Morgan Abigail , Weertman Joshua , Lewis Tommy L 
Published: January 2025

ABSTRACTMitochondrial ATP production and calcium buffering are critical for metabolic regulation and neurotransmission making the formation and maintenance of the mitochondrial network a critical component of neuronal health. Cortical pyramidal neurons contain compartment-specific mitochondrial morphologies that result from distinct axonal and dendritic mitochondrial fission and fusion profiles. We previously showed that axonal mitochondria are maintained at a small size as a result of high axonal mitochondrial fission factor (Mff) activity. However, loss of Mff activity had little effect on cortical dendritic mitochondria, raising the question of how fission/fusion balance is controlled in the dendrites. Thus, we sought to investigate the role of another fission factor, fission 1 (Fis1), on mitochondrial morphology, dynamics and function in cortical neurons. We knocked down Fis1 in cortical neurons both in primary culture andin vivo, and unexpectedly found that Fis1 depletion decreased mitochondrial length in the dendrites, without affecting mitochondrial size in the axon. Further, loss of Fis1 activity resulted in both increased mitochondrial motility and dynamics in the dendrites. These results argue Fis1 exhibits dendrite selectivity and plays a more complex role in neuronal mitochondrial dynamics than previously reported. Functionally, Fis1 loss resulted in reduced mitochondrial membrane potential, increased sensitivity to complex III blockade, and decreased mitochondrial calcium uptake during neuronal activity. The altered mitochondrial network culminated in elevated resting calcium levels that increased dendritic branching but reduced spine density. We conclude that Fis1 regulates morphological and functional mitochondrial characteristics that influence dendritic tree arborization and connectivity.
Nucleus accumbens dopamine encodes the trace period during appetitive Pavlovian conditioning
Authors: Wanat Matthew J. , Camacho Erika T. , Phillips Paul E.M. 
Published: January 2025

AbstractPavlovian conditioning tasks have been used to identify the neural systems involved with learning cue-outcome relationships. In delay conditioning, the presentation of a conditioned stimulus (CS) overlaps or co-terminates with the delivery of the unconditioned stimulus (US). Prior studies demonstrate that behavioral responding during delay conditioning is regulated by dopamine signaling in the nucleus accumbens (NAc). In particular, the dopamine response to the CS reflects the relative value of the upcoming reward in these tasks. In contrast to delay conditioning, trace conditioning involves a ‘trace’ period that separates the end of the CS and the delivery of the US. While dopamine has been implicated in trace conditioning, no studies to date have examined how NAc dopamine responds to reward-related stimuli in these tasks. To address this, we developed a within-subject trace conditioning task where distinct audio CSs signaled either a short trace period (5s) or a long trace period (55s) prior to the delivery of a food reward. Male rats exhibited higher levels of conditioned responding and a faster latency to respond to the Short Trace CS relative to the Long Trace CS. Voltammetry recordings in the NAc found that dopamine levels to the CS increased on Short Trace trials but decreased on Long Trace trials. Conversely, US-evoked dopamine responses were greater on Long Trace trials relative to Short Trace trials. We found that the CS dopamine response correlated with the latency to respond and not to the level of conditioned responding. Furthermore, the relationship between CS dopamine and latency was best explained by an exponential function. Taken together, our results illustrate how CS-evoked dopamine signals in the NAc relate to behavioral outcomes. Furthermore, we find that trace period is encoded by the bidirectional NAc dopamine response to the CS during Pavlovian conditioning.Significance statementLearning how to associate a given cue with an outcome is fundamental process underlying reward seeking behavior. Dopamine in the ventral striatum is important for establishing the link between cues and rewarding outcomes in Pavlovian conditioning tasks. However, it is unclear how striatal dopamine release responds to cues during trace conditioning when there is temporal gap between the cue and the rewarding outcome. To address this, we performed voltammetry recordings of dopamine release in male rats trained on trace conditioning task. We demonstrate that cue-evoked dopamine signals encode the trace period and is related to the latency to respond. While prior reports find that the relative reward value is signaled by increases in dopamine neuron activity to cues, the current study highlights the that dopamine response to reward-predictive cues can signal the relative reward value through bidirectional changes in dopamine transmission.
Maturase K forms a plastidial splicing complex with a neofunctionalized branching enzyme
Authors: Liang Yuanyuan , Gao Yang , Fontana Andrea , Abt Melanie R. , Gicgier Adam P. , Liu Chun , Sharma Mayank , Zoschke Reimo , Zeeman Samuel C. , Pfister Barbara 
Published: January 2025

AbstractChloroplast group IIA introns derive from bacterial ribozymes. Their splicing likely requires Maturase K (MatK), which has been largely inaccessible to functional analyses being itself a chloroplast intron-encoded protein. Here we show that MatK physically interacts with a conserved, essential plastid-localized homolog of starch-branching enzymes (BEs), dubbed MATURASE K INTERACTING PROTEIN1 (MKIP1). We demonstrate that MKIP1 proteins have lost BE activity and acquired an insertion enabling direct interaction with the N-terminal region of MatK. Arabidopsis MKIP1 specifically co-precipitates all known intron targets of MatK. InducedMKIP1silencing results in pale newly emerging leaves, in which the splicing of these intron targets is strongly reduced. Our data suggest that MKIP1 functionally diverged from canonical BEs to facilitate splicing in conjunction with MatK. We propose that the N-terminus of MatK, in turn, has evolved from an RNA-binding domain into a platform for protein interaction, helping its transition towards a general splicing factor.
Search for
hb(2P)→γχbJ(1P)
at
s=10.860  GeV
Authors: Boschetti A. , Mussa R. , Tamponi U. , Adachi I. , Aihara H. , Asner D. M. , Aushev T. , Ayad R. , Banerjee Sw. , Belous K. , Bennett J. , Bessner M. , Biswas D. , Bobrov A. , Bodrov D. , Bozek A. , Bračko M. , Branchini P. , Browder T. E. , Budano A. , Chang M.-C. , Cheon B. G. , Chilikin K. , Cho K. , Choi S.-K. , Choi Y. , Choudhury S. , De Nardo G. , De Pietro G. , Dhamija R. , Di Capua F. , Doležal Z. , Dong T. V. , Ecker P. , Epifanov D. , Ferlewicz D. , Fulsom B. G. , Garg R. , Gaur V. , Garmash A. , Giri A. , Goldenzweig P. , Graziani E. , Gu T. , Guan Y. , Gudkova K. , Hadjivasiliou C. , Hara T. , Hayasaka K. , Hayashii H. , Hazra S. , Hou W.-S. , Hsu C.-L. , Inami K. , Ipsita N. , Itoh R. , Iwasaki M. , Jacobs W. W. , Jin Y. , Kawasaki T. , Kiesling C. , Kim C. H. , Kim D. Y. , Kim K.-H. , Kim Y.-K. , Kinoshita K. , Kodyš P. , Korpar S. , Kovalenko E. , Križan P. , Krokovny P. , Kumar R. , Kumara K. , Kwon Y.-J. , Lam T. , Levit D. , Li L. K. , Li Y. B. , Li Gioi L. , Liventsev D. , Ma Y. , Masuda M. , Matsuda T. , Matvienko D. , Meier F. , Merola M. , Miyabayashi K. , Mizuk R. , Mohanty G. B. , Nakao M. , Natkaniec Z. , Natochii A. , Nayak L. , Nayak M. , Nishida S. , Ogawa S. , Ono H. , Pakhlova G. , Park J. , Park S.-H. , Passeri A. , Patra S. , Paul S. , Pedlar T. K. , Pestotnik R. , Piilonen L. E. , Podobnik T. , Prencipe E. , Prim M. T. , Rout N. , Russo G. , Sandilya S. , Santelj L. , Savinov V. , Schnell G. , Schwanda C. , Seino Y. , Senyo K. , Shan W. , Shen C. P. , Shiu J.-G. , Sokolov A. , Solovieva E. , Starič M. , Sumihama M. , Takizawa M. , Tanida K. , Tenchini F. , Tiwary R. , Uchida M. , Unno Y. , Uno S. , Vinokurova A. , Wang E. , Wang M.-Z. , Wang X. L. , Won E. , Yabsley B. D. , Yelton J. , Yin J. H. , Yook Y. , Yuan L. ,   Belle Collaboration
Published: January 2025

In the bottomonium sector, the hindered magnetic dipole transitions between P-wave states hb(2P)→χbJ(1P)γ, J=0, 1, 2, are expected to be severely suppressed according to the relativized quark model, due to the spin flip of the b quark. Nevertheless, a recent model following the coupled-channel approach predicts the corresponding branching fractions to be enhanced by orders of magnitude. In this Letter, we report the first search for such transitions. We find no significant signals and set upper limits at 90% confidence level on the corresponding branching fractions: B[hb(2P)→γχb0(1P)]<2.7×10−1, B[hb(2P)→γχb1(1P)]<5.4×10−3 and B[hb(2P)→γχb2(1P)]<1.3×10−2. These values help to constrain the parameters of the coupled-channel models. The results are obtained using a 121.4  fb−1 data sample taken around s=10.860  GeV with the Belle detector at the KEKB asymmetric-energy e+e− collider.

Published by the American Physical Society
2025
Influence tactics of broadcasters and consumer purchase intention on live streaming social commerce: approach-avoidance motivation theory
Authors: Dang-Van Thac , Nguyen Nguyen Phong 
Published: January 2025

PurposeThis study uses the approach-avoidance motivation theory (AAMT) to investigate how various influence tactics used by broadcasters affect consumers’ promotion focus and purchase intention in the context of live-streaming social commerce. This study also examines the moderating role of broadcasters’ physical appearance in the relationship between consumer promotion focus and purchase intention.Design/methodology/approachData were collected from 810 consumers on the Taobao live-streaming platform in China. Multivariate techniques and structural equation modeling were used to analyze data and test the research hypotheses.FindingsResults indicate that both rational and emotional influence tactics positively influence consumers’ promotion focus and purchase intention. However, the influences of coercive tactics are mixed: while promise tactics positively influence consumers’ promotion focus and purchase intention, threat tactics have a negative affect. Furthermore, broadcasters’ physical appearance positively moderates the relationship between consumers’ promotion focus and purchase intention.Originality/valueThis study extends the AAMT by proposing and empirically testing a novel model that explains the effects of broadcasters’ influence tactics and physical appearance on consumer behavior within live-streaming social commerce. The findings provide new insights into how broadcasters’ strategies and attributes drive consumers’ positive motivation and behaviors. These insights are valuable for academic researchers and business managers in making informed decisions about recruiting and using broadcasters to achieve favorable consumer outcomes in live-streaming social commerce.