bluuebunny/crossref_metadata_2025_split huggingface dataset:
Variations in precipitation and temperature in Xinjiang (Northwest China) and their connection to atmospheric circulation
Authors: Zhou Guixiang , Chen Yaning , Yao Junqiang 
Published: January 2023

As one of the most vulnerable types of global ecosystems and water resource systems, arid regions are most sensitive to climate change. The Xinjiang (XJ) region is an important part of the arid region in Central Asia and is representative of global arid regions. The complex topography and underlying surface result in distinct climate change characteristics in XJ. In this study, XJ was divided into five sub-regions: the Irtysh River Basin (IRB), the economic belt on the northern slope of the Tianshan Mountains (NSTM), the Ili River Basin (ILRB), the Turpan-Hami Basin (THB), and the Tarim River Basin (TRB). The change in temperature and precipitation over XJ and its sub-regions were investigated from 1960 to 2019 using the Mann-Kendall method and cross-wavelet analysis. Moreover, the multi-timescale correlations between the variations in temperature and precipitation and the atmospheric circulation indices were explored. The results show significant warming and wetting trends in XJ from 1960 to 2019. The warming rate was 0.32°C/10 a (p &lt; 0.01), with an abrupt change during the mid-1990s. The increasing rate of precipitation was 9.24 mm/10 a (p &lt; 0.01), with an abrupt change during the middle to late 1990s. In terms of seasonal variation, the greatest warming rate was during winter (0.37°C/10 a), whereas the precipitation increase was concentrated in summer (3.48 mm/10 a). In terms of spatial variation, a significant warming trend was observed in THB, IRB, ILRB, and NSTM, and precipitation increased significantly in ILRB, NSTM, and the western TRB in southern XJ. The Hurst index analysis indicated that the warming and wetting trends in XJ will slow in the future. Climate change in XJ was closely related to atmospheric circulation at multiple timescales. The subtropical high, Northern-Hemisphere polar vortex activities and the Tibetan Plateau have a significant impact on climate change in XJ. The annual mean temperature in XJ was positively correlated with the area and intensity index of the subtropical high over North Africa, Atlantic, and North America, and negatively correlated with the area and intensity index of the Asia polar vortex. The XJ annual precipitation was positively correlated with the index of the Tibet Plateau Region one and negatively correlated with the intensity index of the Atlantic and European polar vortex, and the area and intensity index of the Northern Hemisphere polar vortex. The results of this study can provide some references for the scientific assessment and accurate prediction of climate change in XJ.
Effects of single and combined applications of three root exudates of Sedum plumbizincicola on the phytoremediation efficiency of paddy soil contaminated with Cd
Authors: Xing Qianwen , Cao Xueying , Tan Changyin , Sun Lijuan , Deng Yueqiang , Yang Jia , Tu Chen 
Published: January 2023

Root exudates are carriers for the transfer of material, energy and information between plant roots and soils. Plants encountering environmental stresses such as heavy metal pollution adapt to the environment by producing and secreting root exudates. In this study, laboratory soil culture experiment and pot experiment with Sedum plumbizincicola were used to study the effects of single and combined application of three root exudates, citric acid, glycine, and fructose, on the Cd-activation and phytoremediation of Cd-contaminated paddy soil. Results from the soil culture experiment showed that for the single application of root exudates, all three root exudates significantly activated the Cd in soil as presented by the increased content of diethylenetriamine pentaacetic acid extracted Cd (DTPA-Cd). In Particular, citric acid (SC) at a relatively low concentration (2 mmol/kg) exhibited the best Cd activation efficiency by increasing DTPA-Cd in the soil by 66.12%. For the combined application of root exudates, citric acid in combination with glycine (SC + G, 1:3) had the best activation effect on the Cd in the soil. In the phytoremediation pot experiment, both the single application of citric acid at a low concentrate (1 mmol/kg) and the combined application of citric acid and glycine (1:1) significantly reduced the total Cd and DTPA-Cd in the soil and increased the biomass and the content of Cd in S. plumbizincicola; thus, the phytoremediation efficiency of Cd-contaminated soil increased by 42.33% and 35.61%. The results from this study suggest that citric acid plays a crucial role in Cd activation and phytoremediation with single or combined applications with glycine. However, the mechanisms under the synergetic interaction between citric acid and glycine require further investigation.
A new method for classifying maize by combining the phenological information of multiple satellite-based spectral bands
Authors: Peng Qiongyan , Shen Ruoque , Dong Jie , Han Wei , Huang Jianxi , Ye Tao , Zhao Wenzhi , Yuan Wenping 
Published: January 2023

Introduction: Using satellite data to identify the planting area of summer crops is difficult because of their similar phenological characteristics.Methods: This study developed a new method for differentiating maize from other summer crops based on the revised time-weighted dynamic time warping (TWDTW) method, a phenology-based classification method, by combining the phenological information of multiple spectral bands and indexes instead of one single index. First, we compared the phenological characteristics of four main summer crops in Henan Province of China in terms of multiple spectral bands and indexes. The key phenological periods of each band and index were determined by comparing the identification accuracy based on the county-level statistical areas of maize. Second, we improved the TWDTW distance calculation for multiple bands and indexes by summing the rank maps of a single band or index. Third, we evaluated the performance of a multi-band and multi-period TWDTW method using Sentinel-2 time series of all spectral bands and some synthetic indexes for maize classification in Henan Province.Results and Discussion: The results showed that the combination of red edge (740.2 nm) and short-wave infrared (2202.4 nm) outperformed all others and its overall accuracy of maize planting area was about 91.77% based on 2431 field samples. At the county level, the planting area of maize matched the statistical area closely. The results of this study demonstrate that the revised TWDTW makes effective use of crop phenological information and improves the extraction accuracy of summer crops’ planting areas over a large scale. Additionally, multiple band combinations are more effective for summer crops mapping than a single band or index input.
Epigenome-wide association studies of meat traits in Chinese Yorkshire pigs highlights several DNA methylation loci and genes
Authors: Wang Kai , Wang Shujie , Ji Xiang , Chen Dong , Shen Qi , Yu Yang , Wu Pingxian , Li Xuewei , Tang Guoqing 
Published: January 2023

In this study, we aimed to identified CpG sites at which DNA methylation levels are associated with meat quality traits in 140 Yorkshire pigs, including pH at 45 min (pH45min), pH at 24 h (pH24h), drip loss (DL), meat redness value (a*), yellowness (b*) and lightness (L*). Genome-wide methylation levels were measured in muscular tissue using reduced representation bisulfite sequencing (RRBS). Associations between DNA methylation levels and meat quality traits were examined using linear mixed-effect models that were adjusted for gender, year, month and body weight. A Bonferroni-corrected p-value lower than 7.79×10−8 was considered statistically significant threshold. Eight CpG sites were associated with DL, including CpG sites annotated to RBM4 gene (cpg301054, cpg301055, cpg301058, cpg301059, cpg301066, cpg301072 and cpg301073) and NCAM1 gene (cpg1802985). Two CpG sites were associated with b*, including RNFT1 and MED13 (cpg2272837) and TRIM37 gene (cpg2270611). Five CpG sites were associated with L*, including GSDMA and LRRC3C gene (cpg2252750) and ENSSSCG00000043539 and IRX1 gene (cpg2820178, cpg2820179, cpg2820181 and cpg2820182). No significant associations were observed with pH45min, pH24h or a*. We reported associations of meat quality traits with DNA methylation and identified some candidate genes associated with these traits, such as NCAM1, MED13 and TRIM37 gene. These results provide new insight into the epigenetic molecular mechanisms of meat quality traits in pigs.
The prognosis difference between elderly and younger patients with adrenocortical carcinoma
Authors: He Shengyin , Huang Xuemei , Zhao Pan , Zhang Peng 
Published: January 2023

Background and aim: Adrenocortical carcinoma (ACC) is uncommon in the elderly. This study aimed to compare the surgical prognosis and survival between senior and younger patients. We also explored the factors that were independently related to the survival of elderly patients.Methods: We identified ACC patients between 2010 and 2019 in the Surveillance, Epidemiology, and End Results (SEER) database and applied Kaplan-Meier curves to evaluate the overall survival (OS) and cancer-specific survival (CSS) with log-rank tests. We also used Cox regression analysis to estimate the OS and CSS. The Fine and Gray model with the Gray test was used to measure the cumulative incidence function (CIF) of CSS and other mortality causes of patients in a competing-risks setting.Results: Of 876 patients, 44.06% were elderly. A lower proportion of elderly patients underwent surgery, regional lymph node surgery, and chemotherapy than young patients. Elderly patients also had inferior OS and CSS than younger patients. The 1- and 5-year OS of elderly patients who underwent surgery were 68% [95% confidence interval (CI): 62%–74%] and 30% (95% CI: 24%–38%), and the 1- and 5-year CSS were 73% (95% CI: 67%–80%) and 40% (95% CI: 32%–47%). The factors independently related to worsened survival included age ≥60 [Hazard Ratio (HR): 1.47 (1.24–1.75)], metastatic disease [HR: 1.90 (1.49–2.51)], higher grade [HR: 1.94 (1.08–3.46)] and Network for the Study of Adrenal Tumors (ENSAT) stage [HR: 1.99 (1.48–2.66)].Conclusion: Younger ACC patients had better survival than the elderly. Factors independently related to worsened survival in elderly patients included age ≥60, metastatic disease, higher grade, and European ENSAT stage.
Pan-cancer analyses reveal IGSF10 as an immunological and prognostic biomarker
Authors: Zhou Yongxia , Gao Manzhi , Jing Yaoyao , Wang Xiaofang 
Published: January 2023

Background: IGSF10 is a member of the immunoglobulin superfamily. Over the previous decade, growing proof has validated definitive correlations between individuals of the immunoglobulin superfamily and human diseases. However, the function of IGSF10 in pan-cancer stays unclear. We aimed to analyze the immunological and prognostic value of IGSF10 in pan-cancer.Methods: We utilized a vary of bioinformatic ways to inspect the function of IGSF10 in pan-cancer, including its correlation with prognosis, immune cell infiltration, tumor mutational burden (TMB), microsatellite instability (MSI), mismatch repair (MMR), DNA methyltransferases, genetic alteration, drug sensitivity, etc.Results: We noticed low expression of IGSF10 in most cancer types. IGSF10 expression in tumor samples correlates with prognosis in most cancers. In most cancer types, IGSF10 expression was strongly related to immune cells infiltration, immune checkpoints, immune modulators, TMB, MSI, MMR, and DNA methyltransferases, among others. Functional enrichment analyses indicated that IGSF10 expression was involved in lymphocyte differentiation, cell molecules adhesion, etc. Furthermore, low IGSF10 expression could increase the drug sensitivity of many drugs.Conclusion:IGSF10 could serve as a novel prognostic marker and attainable immunotherapy target for several malignancies.
Sweetpotato cultivars responses to interactive effects of warming, drought, and elevated carbon dioxide
Authors: Taduri Shasthree , Bheemanahalli Raju , Wijewardana Chathurika , Lone Ajaz A. , Meyers Stephen L. , Shankle Mark , Gao Wei , Reddy K. Raja 
Published: January 2023

Plants are sensitive to changes projected in climates, such as elevated carbon dioxide (eCO2), high temperature (T), and drought stress (DS), which affect crop growth, development, and yield. These stresses, either alone or in combination, affect all aspects of sweetpotato plant growth and development, including storage root development and yield. We tested three sweetpotato cultivars (Beauregard, Hatteras, and LA1188) responses to eight treatments (Control, DS, T, eCO2, DS + T, T + eCO2,DS + eCO2,DS + T + eCO2). All treatments were imposed 36 days after transplanting (DAP) and continued for 47 days. Treatments substantially affected gas exchange, photosynthetic pigments, growth, and storage root components. Cultivars differed considerably for many of the measured parameters. The most significant negative impact of DS was recorded for the shoot and root weights. The combination of DS + T had a significant negative effect on storage root parameters. eCO2alleviated some of the damaging effects of DS and high T in sweetpotato. For instance, eCO2alone or combined with DS increased the storage root weights by 22% or 42% across all three cultivars, respectively. Based on the stress response index, cultivar “Hatteras” was most tolerant to individual and interactive stresses, and “LA 1188” was sensitive. Our findings suggest that eCO2negates the negative impact of T or DS on the growth and yield of sweetpotato. We identified a set of individual and interactive stress-tolerant traits that can help select stress cultivars or breed new lines for future environments.
Full-length transcriptome of in Medicago sativa L. roots in response to drought stress
Authors: Fang Zhihong , Liu Jianning , Wu Xinming , Zhang Yan , Jia Huili , Shi Yonghong 
Published: January 2023

Background: Alfalfa (Medicago sativa L.), serves as a legume with high drought tolerance, is a major forage crop with a high biomass of production. However, the molecular mechanism of Alfalfa in response to drought stress are still unclear.Results: We constructed the first full-length transcriptome for Alfalfa root. 21.53Gb clean data were obtained by further data filtering, in which incorporate 566,076 reads of Insert (ROI), and 409,291 full length reads non-Chimeric (FLNC) sequences. Combined with second-generation sequencing (SGS), there were 2615, 6011, and 4617 differentially expressed genes (DEGs) in three comparisons. KEGG pathway analysis showed enrichment of ribosome, glutathione metabolism, and biosynthesis of amino acids are among the DEGs. The majority of transcription factors (TFs) from DEGs were AP2/ERF-ERF (37), C2H2 (32), and bHLH (22) bZIP (22), followed by C3H (19), MYB (18), WRKY (18), GRAS (16), and NAC (15). 32 C2H2 genes were differentially expressed in three groups. In addition, TFs annotated as C3H (19), MYB (18), GRAS (16), and NAC (15) also changed significantly in expression in the three comparisons. We found 24 genes participate in the abscisic acid (ABA) and auxin hormone signaling pathway in response to drought stress, and monitored the expression patterns of these related genes.Conclusion: The present study enhanced our understanding of the genetic diversity and complexity, and provides greater insight into the fundamental transcriptome reprogramming of Alfalfa under drought.
Improper preanalytical processes on peripheral blood compromise RNA quality and skew the transcriptional readouts of mRNA and LncRNA
Authors: He Yinli , Dong Lele , Yi Hongyang , Zhang Linpei , Shi Xue , Su Lin , Gan Baoyu , Guo Ruirui , Wang Yawen , Luo Qinying , Li Xiaojiao 
Published: January 2023

Genetic and epigenetic reprogramming caused by disease states in other tissues is always systemically reflected in peripheral blood leukocytes (PBLs). Accurate transcriptional readouts of Messenger RNA (mRNA) and Long non-coding RNA (lncRNA) in peripheral blood leukocytes are fundamental for disease-related study, diagnosis and treatment. However, little is known about the impact of preanalytical variables on RNA quality and downstream messenger RNA and Long non-coding RNA readouts. In this study, we explored the impact of RNA extraction kits and timing of blood placement on peripheral blood leukocyte-derived RNA quality. A novel enhanced evaluation system including RNA yields, purity, RNA integrity number (RIN) values and β-actin copies was employed to more sensitively identify RNA quality differences. The expression levels of informative mRNAs and Long non-coding RNAs in patients with chronic obstructive pulmonary disease (COPD) or triple-negative breast cancer (TNBC) were measured by Quantitative reverse transcription polymerase chain reaction (qRT–PCR) to investigate the impact of RNA quality on transcriptional readouts. Our results showed that the quality of RNA extracted by different kits varies greatly, and commercial kits should be evaluated and managed before batch RNA extraction. In addition, the quality of extracted RNA was highly correlated with the timing of blood placement, and the copy number of β-actin was significantly decreased after leaving blood at RT over 12 h. More importantly, compromised RNA leads to skewed transcriptional readouts of informative mRNAs and Long non-coding RNAs in patients with chronic obstructive pulmonary disease or triple-negative breast cancer. These findings have significant implications for peripheral blood leukocyte-derived RNA quality management and suggest that quality control is necessary prior to the analysis of patient messenger RNA and Long non-coding RNA expression.
Screening and identifying a novel M-MDSCs-related gene signature for predicting prognostic risk and immunotherapeutic responses in patients with lung adenocarcinoma
Authors: Wang Geng-Chong , Zhou Mi , Zhang Yan , Cai Hua-Man , Chiang Seok-Theng , Chen Qi , Han Tian-Zhen , Li Rong-Xiu 
Published: January 2023

Background: Lung adenocarcinoma (LUAD) shows intratumoral heterogeneity, a highly complex phenomenon that known to be a challenge during cancer therapy. Considering the key role of monocytic myeloid-derived suppressor cells (M-MDSCs) in the tumor microenvironment (TME), we aimed to build a prognostic risk model using M-MDSCs-related genes.Methods: M-MDSCs-related genes were extracted from The Cancer Genome Atlas (TCGA) and Gene Expression Omnibus (GEO) databases. Utilized univariate survival analysis and random forest algorithm to screen candidate genes. A least absolute shrinkage and selection operator (LASSO) Cox regression analysis was selected to build the risk model. Patients were scored and classified into high- and low-risk groups based on the median risk scores. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis along with R packages “estimate” and “ssGSEA” were performed to reveal the mechanism of risk difference. Prognostic biomarkers and tumor mutation burden (TMB) were combined to predict the prognosis. Nomogram was carried out to predict the survival probability of patients in 1, 3, and 5 years.Results: 8 genes (VPREB3, TPBG, LRFN4, CD83, GIMAP6, PRMT8, WASF1, and F12) were identified as prognostic biomarkers. The GEO validation dataset demonstrated the risk model had good generalization effect. Significantly enrichment level of cell cycle-related pathway and lower content of CD8+ T cells infiltration in the high-risk group when compared to low-risk group. Morever, the patients were from the intersection of high-TMB and low-risk groups showed the best prognosis. The nomogram demonstrated good consistency with practical outcomes in predicting the survival rate over 1, 3, and 5 years.Conclusion: The risk model demonstrate good prognostic predictive ability. The patients from the intersection of low-risk and high-TMB groups are not only more sensitive response to but also more likely to benefit from immune-checkpoint-inhibitors (ICIs) treatment.
Feasibility and acceptability of an adapted environmental enrichment intervention for endometriosis: A pilot study
Authors: Nieves-Vázquez Cristina I. , Detrés-Marquéz Amanda C. , Torres-Reverón Annelyn , Appleyard Caroline B. , Llorens-De Jesús Astrid P. , Resto Ivana N. , López-Rodríguez Verónica , Ramos-Echevarría Paola M. , Castro Eida M. , Flores Idhaliz 
Published: January 2023

IntroductionWe have previously shown that Environmental Enrichment (EE)-consisting of social support, novelty, and open spaces—decreased disease progression and anxiety in a rat model of endometriosis. We developed a novel EE intervention to be tested in a pilot randomized clinical trial (RCT) in patients with endometriosis, a painful, stressful disease.ObjectiveTo translate and evaluate the feasibility and acceptability of an adapted EE intervention as an adjuvant to standard-of-care for endometriosis patients.MethodsFeasibility was assessed through recruitment, enrollment, and adherence rates. Acceptability was evaluated through a post-intervention survey and focus group discussion 3-months after the end of the intervention.ResultsOf the 103 subjects recruited, 64 were randomized to the intervention group and 39 to the control group. At the start of the intervention, the study groups consisted of 29 (intervention) and 27 (control) subjects. Enrollment rates were 45.3% and 69.2%, and adherence rates were 41.4% and 100% for the intervention and control groups, respectively. Delays resulting from natural events (earthquakes, the COVID-19 pandemic) impacted enrollment and adherence rates. The most common reasons for missing an intervention were period pain (39.1%) and work-study (34.8%). There was high acceptability (&gt;80%) of the intervention's logistics. The majority (82.4%) of subjects would continue participating in support groups regularly, and 95.7% would recommend the intervention to other patients.ConclusionsWe showed that EE could be translated into an acceptable integrative multi-modal therapy perceived as valuable among participants who completed the intervention. High attrition/low adherence indicates that additional refinements would be needed to improve feasibility. Acceptability data indicate that EE has the potential to be integrated into the clinical management of patients with endometriosis and other inflammatory, painful disorders. Studies are ongoing to assess the efficacy of EE in improving pain symptoms, mental health, and quality of life (QoL).