bluuebunny/crossref_metadata_2025_split huggingface dataset:
Senescence-related receptor kinase 1 functions downstream of WRKY53 in regulating leaf senescence in Arabidopsis
Authors: Wang Qi , Li Xiaoxu , Guo Cun , Wen Lichao , Deng Zhichao , Zhang Zenglin , Li Wei , Liu Tao , Guo Yongfeng 
Published: June 2023

Abstract
Receptor-like kinases (RLKs) are the most important class of cell surface receptors, and play crucial roles in plant development and stress responses. However, few studies have been reported about the biofunctions of RLKs in leaf senescence. Here, we characterized a novel Arabidopsis RLK-encoding gene, SENESCENCE-RELATED RECEPTOR KINASE 1 (SENRK1), which was significantly down-regulated during leaf senescence. Notably, the loss-of-function senrk1 mutants displayed an early leaf senescence phenotype, while overexpression of SENRK1 significantly delayed leaf senescence, indicating that SENRK1 negatively regulates age-dependent leaf senescence in Arabidopsis. Furthermore, the senescence-promoting transcription factor WRKY53 repressed the expression of SENRK1. While the wrky53 mutant showed a delayed senescence phenotype as previously reported, the wrky53 senrk1-1 double mutant exhibited precocious leaf senescence, suggesting that SENRK1 functions downstream of WRKY53 in regulating age-dependent leaf senescence in Arabidopsis.
Rotational spectra and astronomical search of five ethynyl derivatives of propene
Authors: Cabezas Carlos , Agúndez Marcelino , Liu Yi-Ting , Endo Yasuki , Cernicharo José 
Published: June 2023

ABSTRACT
The interstellar discovery of the five cyano derivatives of propene brings interest in analogous molecules that could be detected in similar astronomical environments. In this context, ethynyl-propene derivatives are good candidates to be observed in the Taurus Molecular Cloud (TMC-1), where other cyano and ethynyl derivatives of hydrocarbons such as ethane, allene, propene, and cyclopentadiene, among others, have been detected. We have investigated the pure rotational spectra of the five ethynyl-propene derivatives to enable their interstellar detections. These molecular species have been produced by an electric discharge and their rotational spectra were characterized using a Balle–Flygare narrow band-type Fourier-transform microwave spectrometer operating in the frequency region of 4-40 GHz. The spectral analysis allowed us to derive accurate spectroscopic parameters to obtain reliable frequency predictions for astronomical searches. We do not detect any of these species in TMC-1 but we derive upper limits for the abundances for the five isomers.
Exotica in the globular cluster M4, studied with Chandra, HST, and the VLA
Authors: Lugger Phyllis M , Cohn Haldan N , Heinke Craig O , Zhao Jiaqi , Zhao Yue , Anderson Jay 
Published: June 2023

ABSTRACT
Using the Hubble Ultraviolet Globular Cluster Survey (HUGS) and additional HST archival data, we have carried out a search for optical counterparts to the low-luminosity Chandra X-ray sources in the globular cluster M4 (NGC 6121). We have also searched for optical or X-ray counterparts to radio sources detected by the VLA. We find 24 new confident optical counterparts to Chandra sources for a total of 40, including the 16 previously identified. Of the 24 new identifications, 18 are stellar coronal X-ray sources (active binaries, ABs), the majority located along the binary sequence in a V606–I814 colour–magnitude diagram and generally showing an H α excess. In addition to confirming the previously detected cataclysmic variable (CV, CX4), we identify one confident new CV (CX76), and two candidates (CX81 and CX101). One MSP is known in M4 (CX12), and another strong candidate has been suggested (CX1); we identify some possible MSP candidates among optical and radio sources, such as VLA20, which appears to have a white dwarf counterpart. One X-ray source with a sub-subgiant optical counterpart and a flat radio spectrum (CX8, VLA31) is particularly mysterious. The radial distribution of X-ray sources suggests a relaxed population of average mass $\sim 1.2\!-\! 1.5\, \mbox{$\mathrm{ M_\odot} $}$. Comparing the numbers of ABs, MSPs, and CVs in M4 with other clusters indicates that AB numbers are proportional to cluster mass (primordial population), MSPs to stellar encounter rate (dynamically formed population), while CVs seem to be produced both primordially and dynamically.
Program Development and Grant Writing
Authors: Nair Murali , Marquart Matthea , Young Lindsay 
Published: June 2023

Program Development and Grant Writing are integral elements in human services and in other diverse fields. Social work practitioners in the twenty-first century work in complex areas of program development and grant writing. Public and nonprofit organizations are attempting to equip themselves with the latest technology to cope with these new challenges. This article includes a combination of multidisciplinary resources on program development and grant writing, and those that have a specific focus on social welfare. These bibliographies identify innovative social welfare program development and grant writing methodologies in social work. The references and resources here were selected based on their unique quality as well as their diverse differences. To illustrate the development of the field, the annotated references and media sites mentioned include various case studies from a multidisciplinary perspective. Students and professionals in the human services field at any level can draw upon this resource to enhance their understanding of the practices of Program Development and Grant Writing. Peer-reviewed books, journal articles, and social media sites that describe the expanse of Program Development and Grant Writing are cited in this article.
The Provocation by Witchcraft Defence in Anglophone Africa: Origins and Historical Development
Authors: Owusu Emmanuel Sarpong 
Published: June 2023

Abstract
Provocation by witchcraft, a defence to a homicidal act supposedly perpetrated under the influence of belief in witchcraft and juju, has become a plea frequently invoked by witch-killers in many African countries, particularly those formerly colonized by Britain. Over the last century, the courts in Anglophone Africa have repeatedly been invited to address the question as to whether the belief in witchcraft and juju avails to an accused person the defence of grave provocation and, if so, under what conditions. Yet, very little is known about the origins and nature of this controversial legal defence, and the boundaries of its application remain murky. Drawing on a wide range of relevant academic literature, statutes and, more importantly, case law, the present study investigates the origins of the provocation by witchcraft plea and explores the historical development of this contentious defence, highlighting the extent to which the courts’ perspective on the scope of its application has evolved since the 1930s.
Cyclic anthraquinone derivatives, unique G-quadruplex binders, selectively induce cancer cell apoptosis and inhibit tumor growth
Authors: Fukuda Hikaru , Zou Tingting , Fujii Satoshi , Sato Shinobu , Wakahara Daiki , Higashi Sen , Tseng Ting-Yuan , Chang Ta-Chau , Yada Naomi , Matsuo Kou , Habu Manabu , Tominaga Kazuhiro , Takeuchi Hiroshi , Takenaka Shigeori 
Published: June 2023

AbstractCyclic anthraquinone derivatives (cAQs), which link two side chains of 1,5-disubstituted anthraquinone as a threading DNA intercalator, have been developed as G-quartet (G4) DNA-specific ligands. Among the cAQs, cAQ-mBen linked through the 1,3-position of benzene had the strongest affinity for G4 recognition and stabilization in vitro and was confirmed to bind to the G4 structure in vivo, selectively inhibiting cancer cell proliferation in correlation with telomerase expression levels and triggering cell apoptosis. RNA-sequencing analysis further indicated that differentially expressed genes regulated by cAQ-mBen were profiled with more potential quadruplex-forming sequences. In the treatment of the tumor-bearing mouse model, cAQ-mBen could effectively reduce tumor tissue and had less adverse effects on healthy tissue. These results suggest that cAQ-mBen can be a potential cancer therapeutic agent as a G4 binder.
Drug repurposing screening and mechanism analysis based on human colorectal cancer organoids
Authors: Mao Yunuo , Wang Wei , Yang Jingwei , Zhou Xin , Lu Yongqu , Gao Junpeng , Wang Xiao , Wen Lu , Fu Wei , Tang Fuchou 
Published: June 2023

Abstract
Colorectal cancer (CRC) is a highly heterogeneous cancer and exploring novel therapeutic options is a pressing issue that needs to be addressed. Here, we established human CRC tumor-derived organoids that well represent both morphological and molecular heterogeneities of original tumors. To efficiently identify repurposed drugs for CRC, we developed a robust organoid-based drug screening system. By combining the repurposed drug library and computation-based drug prediction, 335 drugs were tested and 34 drugs with anti-CRC effects were identified. More importantly, we conducted a detailed transcriptome analysis of drug responses and divided the drug response signatures into five representative patterns: differentiation induction, growth inhibition, metabolism inhibition, immune response promotion, and cell cycle inhibition. The anticancer activities of drug candidates were further validated in the established patient-derived organoids-based xenograft (PDOX) system in vivo. We found that fedratinib, trametinib, and bortezomib exhibited effective anticancer effects. Furthermore, the concordance and discordance of drug response signatures between organoids in vitro and pairwise PDOX in vivo were evaluated. Our study offers an innovative approach for drug discovery, and the representative transcriptome features of drug responses provide valuable resources for developing novel clinical treatments for CRC.
Prevalence of LTBI in patients with autoimmune diseases and accuracy of IGRA in predicting TB relapse
Authors: Chandrashekara S , Panchagnula Renuka , Chennupati Yogitha 
Published: June 2023

Abstract

Objectives
The current study evaluated latent tuberculosis infection (LTBI) positivity in a cohort of Indian subjects and the accuracy of IFN-gamma release assay (IGRA) in predicting tuberculosis (TB) reactivation.

Methods
This cross-sectional, retrospective chart-based study considered patients diagnosed with autoimmune rheumatic diseases (AIRDs), especially those who received treatment with biologics or targeted synthetic (ts)DMARDs. The patients had undergone LTBI screening and IGRA test. The study excluded patients with inadequate information and those who had undergone test exclusively for diagnostic purpose. Statistical analyses were carried out for descriptive, demographic and clinical variables. Accuracy and error rate in predicting the absence of TB reactivation were calculated for IGRA test.

Results
The study selected 943 patients who had undergone IGRA pre-screening prior to the initiation of biologics or tsDMARDs with a mean age of 42.93 ± 14.01 years and male-to-female ratio of 1:2.08. RA was the most common primary diagnosis (43.16%). The proportion of subjects who received single, double and triple or more DMARDs or immune suppressants were 54.35%, 33.33% and 7.69%, respectively. Among the selected subjects, 125 patients were LTBI positive and 816 were negative. All patients, except one who tested positive at baseline, received antitubercular prophylaxis. Accuracy of IGRA in predicting the absence of TB reactivation was 99.6%, with an error rate of 0.46.

Conclusion
LTBI screening is beneficial in AIRDs patients prior to the prescription of biologics or tsDMARDs. IGRA is ideal for identifying patients with increased likelihood of developing TB upon receiving biologics or tsDMARDs with reasonable accuracy.
Exploring Mistreatment of Medical Students by Patients: A Qualitative Study
Authors: Hu Amanda , MacDonald Graham , Jain Neera R. , Nimmon Laura 
Published: June 2023

Purpose
Mistreatment of medical students by patients has not been qualitatively explored in the literature. The authors sought to develop a rich understanding of the impact and consequences of medical students’ experiences of mistreatment by patients.

Method
This exploratory descriptive qualitative study was conducted at a large Canadian medical school from April–November 2020. Fourteen medical students were recruited for semistructured interviews. Students were asked about their experiences of mistreatment by patients and how they responded to these experiences. Transcripts were thematically analyzed using an inductive approach, and the authors interwove critical theory into their conceptual interpretation of the data.

Results
Fourteen medical students (median age = 25.5; 10, 71.4% self-reported male; 12, 85.7% self-identified visible minority) participated in this study. Twelve (85.7%) participants had personally experienced patient mistreatment and 2 (14.3%) had witnessed mistreatment of another learner. Medical students described being mistreated by patients based on their gender and race/ethnicity. Although all participants were aware of the institution’s official mechanism for reporting mistreatment, none filed an official report. Some participants described turning to their formal (faculty members and residents) and informal (family and friends) social supports to cope with mistreatment by patients. Participants described resenting and avoiding patients who mistreated them and struggling to maintain empathy for, openness to, and overall ethical engagement with discriminatory patients. Students often described a need to be stoic toward their experiences of mistreatment by patients, often seeing it as their “professional duty” to overcome and thus suppress the negative emotions associated with mistreatment.

Conclusions
Medical schools must proactively develop multifaceted mechanisms to support medical students who experience mistreatment by patients. Future research can further uncover this neglected dimension of the hidden curriculum to better develop responses to incidents of mistreatment that commit to antiracism, antisexism, patient care, and learner care.
Optical coherence tomography characteristics over time in Behçet's uveitis
Authors: Liang Ruyu , Yang Li , Zeng Shun , Liu Xiaoli 
Published: June 2023

ABSTRACT

Purpose:
To analyse the characteristics of macular optical coherence tomography (OCT) in patients with Behçet's uveitis (BU).

Methods:
Retrospectively analyzing OCT images and clinial data of BU patients who visited our hospital from January 2010 to July 2022.

Results:
One hundred and one patients (174 eyes) were included. We analyzed the development of OCT changes in these patients and its relationship with acuity vision, and found that cystic macular oedema, hyperreflexive retinal spots, INL oedema and ONL oedema appeared at any time during the disease course. Epiretinal membranes appeared beginning 1-2 weeks after onset and worsened over time, foveal atrophy occurred beginning at 2-4 weeks. Foveal atrophy, foveal layers disappear, EZ disruption, RPE disruption, RPE hyperreflection, choroidal hyperreflection were correlated with visual acuity. Kaplan‒Meier survival analysis showed at 60 months of follow-up, almost all patients with foveal atrophy, EZ disruption, RPE disruption, RPE hyperreflection, choroidal hyperreflection had visual acuity less than LogMar 1.0.. EZ disruption and RPE disruption was reversible with aggressive treatment from 4-12 months after onset, but was less recoverable after 2 years. In advanced stages, OCT manifested as structural disturbances and atrophy of the macular area, highly reflective material deposition in the RPE layer, and a thick macular epimembrane.

Conclusion:
OCT showed that severe lesions occurred in macular of BU patients in early stage. Aggressive treatment can partially reverse it.
Evaluating the Impact of the 2017 Dutch Neonatal Early-Onset Sepsis Guideline
Authors: Van Rossem Ellen J. D. , Plötz Frans B. , Felderhof Mariet K. 
Published: June 2023

Background:
In 2017, a new Dutch neonatal early-onset sepsis (EOS) guideline was implemented. It is an adaptation from the United Kingdom National Institute for Health and Care Excellence guideline and focuses on maternal and neonatal risk factors. We aim to assess if this guideline performs better at reducing the rate of antibiotic treatment for EOS than the old Dutch categorical EOS guideline, which focused primarily on group B streptococcus (GBS) testing and prophylaxis.

Methods:
We performed a single-center retrospective cohort study in the Netherlands. Data were collected from two 12-month epochs (2015 vs. 2019). Neonates were included when treated for suspected EOS or when observed for an elevated EOS risk.

Results:
The empirical antibiotic rate was 4.6% in both years. Prolonged antibiotic treatment (>48 u) increased from 24% in 2015 to 39% in 2019 (P = 0.021). Adherence to the guideline decreased from 98% in 2015 to 84% in 2019 (P < 0.001). Strict adherence in 2019 would have led to more antibiotic treatment (5.1% instead of 4.6%). The EOS incidence rate was comparable, namely 0.6% in 2015 and 0.0% in 2019 (P = 0.480). The change in the definition of risk factors in 2019 led to less antibiotic treatment in case of a maternal fever during birth, from 48% in 2015 to 26% in 2019 (P < 0.001).

Conclusions:
The new Dutch categorical EOS guideline does not achieve its intended purpose of reducing empiric antibiotic therapy for suspected EOS. We advocate the need for a new screening strategy.
Experimental study comparing 2 different phacoemulsification systems with intraocular pressure control during steady-state flow and occlusion break surge events
Authors: Fanney Douglas , Layser Gregory S. , K Akhil R. , Kohlhammer Susanne , Kübler Christoph , Seibel Barry S. 
Published: June 2023

Purpose:
To compare peak surge and surge duration time after occlusion break, incision leakage compensation, and passive vacuum for 2 phacoemulsification systems.

Setting:
Carl Zeiss Meditec AG, Oberkochen, Germany.

Design:
Laboratory study.

Methods:
A spring-eye model was used to test Alcon Centurion Vision and Zeiss Quatera 700 systems. Peak surge and duration was measured after an occlusion break. Quatera tested in flow and vacuum priority modes. Vacuum limits ranged from 300 to 700 mm Hg with intraocular pressure (IOP) set at 30 mm Hg, 55 mm Hg, and 80 mm Hg. IOP vs incision leakage rates of 0 to 15 cc/min and passive vacuum were measured.

Results:
At 30 mm Hg IOP set point and vacuum limits ranging 300 to 700 mm Hg, the surge duration time after occlusion break ranged 419 to 1740 milliseconds (ms) for Centurion, 284 to 408 ms for Quatera in the flow mode, and 282 to 354 ms for Quatera in the vacuum mode. At 55 mm Hg, values ranged 268 to 1590 ms for Centurion, 258 to 471 ms for Quatera in the flow mode, and 239 to 284 ms for Quatera in the vacuum mode. At 80 mm Hg, values were 243 to 1520 ms for Centurion, 238 to 314 ms for Quatera in the flow mode, and 221 to 279 ms in the vacuum mode. Centurion exhibited slightly less peak surge than the Quatera. At 55 mm Hg: incision leakage rates 0 to 15 cc/min, Quatera held the IOP within ±2 mm Hg of target; Centurion was unable to hold IOP target allowing a 11.7 mm Hg decrease with 32% higher passive vacuum.

Conclusions:
Quatera demonstrated slightly higher surge peak values and notably shorter surge duration times after occlusion break than Centurion. Quatera demonstrated better incision leakage compensation and lower passive vacuum than Centurion.
Perioperative insomnia trajectories and functional outcomes after total knee arthroplasty
Authors: Hamilton Katrina R. , Hughes Abbey J. , Campbell Claudia M. , Owens Michael A. , Pester Bethany D. , Meints Samantha M. , Taylor Janiece L. , Edwards Robert R. , Haythornthwaite Jennifer A. , Smith Michael T. 
Published: June 2023

Abstract
Longitudinal total knee arthroplasty (TKA) studies indicate that a substantial percentage of patients continue to experience clinically significant pain and functional impairment after surgery. Insomnia has been associated with poorer surgical outcomes; however, previous work has largely focused on long-term postsurgical insomnia. This study builds on previous work by examining sleep and pain outcomes about perioperative insomnia trajectories. Insomnia symptoms (using the Insomnia Severity Index) during the acute perioperative period (2 weeks pre-TKA to 6 weeks post-TKA) were used to classify participants into perioperative insomnia trajectories: (1) No Insomnia (ISI < 8), (2) New Insomnia (baseline < 8; postoperative ≥ 8 or ≥6-point increase), (3) Improved Insomnia (baseline ≥ 8, postoperative < 8 or ≥6-point decrease), and (4) Persistent Insomnia (ISI ≥ 8). Insomnia, pain, and physical functioning were assessed in participants with knee osteoarthritis (n = 173; Mage = 65 ± 8.3, 57.8% female) at 5 time points: 2 weeks pre-TKA, post-TKA: 6 weeks, 3 months, 6 months, and 12 months. Significant main effects were seen for insomnia trajectory and time, and trajectory-by-time interactions for postoperative insomnia, pain severity, and physical functioning (P’s < 0.05). The Persistent Insomnia trajectory had the worst postoperative pain at all follow-ups and marked insomnia and physical functioning impairment post-TKA (P’s < 0.05). The New Insomnia trajectory had notable long-term insomnia (6 weeks to 6 months) and acute (6 weeks) postoperative pain and physical functioning (P’s < 0.05). Findings indicated a significant relationship between perioperative insomnia trajectory and postoperative outcomes. Results of this study suggest that targeting presurgical insomnia and preventing the development of acute postoperative insomnia may improve long-term postoperative outcomes, with an emphasis on persistent perioperative insomnia due to poorer associated outcomes.
Ancient DNA in Lycoptera Fossils: Key Discoveries for Evolution and Methodological Reassessment
Authors: Zhao Wan-Qian , Guo Zhan-Yong , Tian Zeng-Yuan , Su Tong-Fu , Cao Gang-Qiang , Qi Zi-Xin , Qin Tian-Cang , Zhou Wei , Yang Jin-Yu , Chen Ming-Jie , Zhang Xin-Ge , Zhou Chun-Yan , Zhu Chuan-Jia , Tang Meng-Fei , Wu Di , Song Mei-Rong , Guo Yu-Qi , Qiu Li-You , Liang Fei , Li Mei-Jun , Geng Jun-Hui , Zhao Li-Juan , Zhang Shu-Jie 
Published: June 2023

AbstractHigh-quality ancient DNA (aDNA) is crucial for advancements in molecular paleontology. However, current research is often limited to fossil samples less than 1 million years old (Ma) due to DNA degradation and contamination by environmental DNA (eDNA). To overcome these limitations, we used nanoparticle affinity beads to successfully extract DNA from Lycoptera davidi fossils dating back to the Early Cretaceous period, approximately 120 Ma. Utilizing high-throughput sequencing technology, we obtained 1,258,901 DNA sequences. To ensure the accuracy and authenticity of these sequences, we established the rigorous “mega screen method.” This approach allowed us to identify 243 originalin situDNA (oriDNA) fragments likely belonging to the Lycoptera genome. These fragments have an average length of over 100 base pairs and show no signs of “deamination”. Additionally, we discovered 10 transposase coding sequences, which provide insights into a unique self-renewal mechanism within the Lycoptera genome. The update of protein-coding sequences was responsible for the significant divergence of ray-finned fishes in the Cretaceous. Surprisingly, we identified numerous hominid aDNA fragments from the fossil extracts. These fragments, probably from the environmental source during later epochs, had been deposited in the voids that formed after the fossil rocks developed. Notably, these fragments also exhibited no signs of “deamination”. This study provides invaluable data to further our understanding of fish evolution. Also, the non-remain DNA evidence offers crucial clues for anthropologists in their quest for undocumented human-like skeletons in various locations. It also shows the potential for transforming the field of molecular paleontology via studying aDNA in exceptional old rocks.