bluuebunny/crossref_metadata_2025_split huggingface dataset:
Targeting Acanthamoeba proteins interaction with flavonoids of Propolis extract by in vitro and in silico studies for promising therapeutic effects
Authors: Sama-ae Imran , Sangkanu Suthinee , Siyadatpanah Abolghasem , Norouzi Roghayeh , Chuprom Julalak , Mitsuwan Watcharapong , Surinkaew Sirirat , Boonhok Rachasak , Paul Alok K. , Mahboob Tooba , Abtahi Najme Sadat , Jimoh Tajudeen O. , Oliveira Sónia M.R. , Gupta Madhu , Sin Chea , de Lourdes Pereira Maria , Wilairatana Polrat , Wiart Christophe , Rahmatullah Mohammed , Dolma Karma G. , Nissapatorn Veeranoot 
Published: February 2023

Background: Propolis is a natural resinous mixture produced by bees. It provides beneficial effects on human health in the treatment/management of many diseases. The present study was performed to demonstrate the anti-Acanthamoeba activity of ethanolic extracts of Propolis samples from Iran. The interactions of the compounds and essential proteins of Acanthamoeba were also visualized through docking simulation. Methods: The minimal inhibitory concentrations (MICs) of Propolis extract against Acanthamoeba trophozoites and cysts was determined in vitro. In addition, two-fold dilutions of each of agents were tested for encystment, excystment and adhesion inhibitions. Three major compounds of Propolis extract such as chrysin, tectochrysin and pinocembrin have been selected in molecular docking approach to predict the compounds that might be responsible for encystment, excystment and adhesion inhibitions of A. castellanii. Furthermore, to confirm the docking results, molecular dynamics (MD) simulations were also carried out for the most promising two ligand-pocket complexes from docking studies. Results: The minimal inhibitory concentrations (MICs) 62.5 and 125 µg/mL of the most active Propolis extract were assessed in trophozoites stage of Acanthamoeba castellanii ATCC30010 and ATCC50739, respectively. At concentrations lower than their MICs values (1/16 MIC), Propolis extract revealed inhibition of encystation. However, at 1/2 MIC, it showed a potential inhibition of excystation and anti-adhesion. The molecular docking and dynamic simulation revealed the potential capability of Pinocembrin to form hydrogen bonds with A. castellanii Sir2 family protein (AcSir2), an encystation protein of high relevance for this process in Acanthamoeba. Conclusions: The results provided a candidate for the development of therapeutic drugs against Acanthamoeba infection. In vivo experiments and clinical trials are necessary to support this claim.
Education programmes on performance-based assessment for allied health and nursing clinical educators: A scoping review protocol
Authors: Alpine Lucy , Barrett Emer , Broderick Julie , Mockler David , O'Connor Anne 
Published: February 2023

Background: Performance-based assessment (PBA) is a complex process undertaken in the workplace by healthcare practitioners known as clinical educators, who assist universities in determining health professional students’ readiness for independent practice. Preparing healthcare professionals for PBA is considered essential to ensuring the quality of the assessment process in the clinical learning environment. A preliminary search of the literature indicated a paucity of research guiding the development of education programmes that support practice educators to understand and implement PBA. Objective: The aim of this scoping review is to investigate and describe education programmes delivered to allied health and nursing clinical educators, to develop PBA knowledge and skills. Methods: This review will follow the Joanna Briggs Institute (JBI) methodology for conducting scoping reviews. Electronic databases relevant to this research topic will be searched including, EMBASE, ERIC, MEDLINE (Ovid), Web of Science and CINAHL and other targeted databases for grey literature. Studies that include PBA as the main focus or a component of the education programmes, of any format, delivered to clinical educators in allied health and nursing will be included. Studies may report the design and/or implementation and/or evaluation of PBA education programmes. Relevant English language publications will be sought from January 2000 to October 2022. Two reviewers will screen all titles and abstracts against the inclusion/exclusion criteria, and publications deemed relevant will be eligible for full text screening, confirming appropriateness for inclusion in the scoping review. Data will be charted to create a table of the results, supported by narrative summary of findings in line with the review objectives.
Creating personas for exposome research: the experience from the HEAP project
Authors: Coombs Heather , Wootton Tracy , Dillner Joakim , Müller Heimo , Berger Anouk , Kozlakidis Zisis 
Published: February 2023

The exposome is a complex scientific field that has enjoyed consistent growth over the last two decades, defined as the composite of every exposure to which an individual is subjected from conception to death. The study of the exposome requires consideration of both the nature of those exposures and their changes over time, and as such necessitates high quality data and software solutions. As the exposome is both a broad and a recent concept, it is challenging to define or to introduce in a structured way. Thus, an approach to assist with clear definitions and a structured framework is needed for the wider scientific and public communication. Results: A set of 14 personas were developed through three focus groups and a series of 14 semi-structured interviews. The focus groups defined the broad themes specific to exposome research, while the sub-themes emerged to saturation via the interviews process. Personas are imaginary individuals that represent segments/groups of real people within a population. Within the context of the HEAP project, the created personas represented both exposome data generators and users. Conclusion: Personas have been implemented successfully in computer science, improving the understanding of human-computer interaction. The creation of personas specific to exposome research adds a useful tool supporting education and outreach activities for a complex scientific field.
Plusy i minusy kształcenia w okresie pandemii dla rodziny i dzieci
Authors: Nowak Marian 
Published: February 2023

Przez ponad rok, ze względu na pandemię, zaistniały w całym świecie specyficzne restrykcje dotyczące życia ludzi i ich funkcjonowania, obejmujące także edukację – w tym wkroczyły one w zasady pracy szkół każdego stopnia i etapu kształcenia, w realizowanie programu zajęć, we wzajemne relacje uczniów i nauczycieli oraz funkcjonowanie instytucji szkolnych. Kształcenie na odległość, dzięki możliwościom stwarzanym przez współczesną technologię, okazywało się bardzo pomocne w zachowaniu ciągłości kształcenia i zapewnieniu ciągłości ścieżki szkoleniowej. Kształcenie takie posiada swoje wady i ograniczenia, ale także można wskazać na szereg zalet, zwłaszcza jeśli się uwzględni uwarunkowania, jakie wymuszała sytuacja w odniesieniu do dzieci jak i rodziców, samej rodziny i też instytucji szkolnych, ich współpracy oraz odkrywania nowych zadań. Nadzwyczajne i nieoczekiwane sytuacje w szkole i w rodzinie, jakie spowodował okres pandemii, to nie tylko zatem cały szereg nowych i dotychczas nieznanych problemów, ale zarazem także impuls do kreatywnego poszukiwania nowych rozwiązań pro-jakościowych i faktycznej troski o jakość kształcenia.
Measurement of individual color space using a luminous vector field
Authors: Alleysson David , Méary David 
Published: February 2023

This study is intended to measure the geometry of the observer’s color space when viewing a computer screen and to define individual variations from these data. A CIE photometric standard observer assumes that the eye’s spectral efficiency function is constant, and photometry measurements correspond to vectors with fixed directions. By definition, the standard observer decomposes color space into planar surfaces of constant luminance. Using heterochromatic photometry with a minimum motion stimulus, we systematically measure the direction of luminous vectors for many observers and many color points. During the measurement process, the background and stimulus modulation averages are fixed to the given points to ensure that the observer is in a fixed adaptation mode. Our measurements result in a vector field or set of vectors (x,v), where x is the point’s color space position, and v is the observer’s luminosity vector. To estimate surfaces from vector fields, two mathematical hypotheses were used: (1) that surfaces are quadratic or, equivalently, that the vector field model is affine, and (2) that the metric of surfaces is proportional to a visual origin. Across 24 observers, we found that vector fields are convergent and the corresponding surfaces are hyperbolic. The equation of the surface in the display’s color space coordinate system, and in particular the axis of symmetry, varied systematically from individual to individual. A hyperbolic geometry is compatible with studies that emphasize a modification of the photometric vector with changing adaptations.
Two-dimensional freeform reflector design with a scattering surface
Authors: Kronberg Vì C. E. , Anthonissen Martijn J. H. , ten Thije Boonkkamp Jan H. M. , IJzerman Wilbert L. 
Published: February 2023

We combine two-dimensional freeform reflector design with a scattering surface modeled using microfacets, i.e., small, specular, surfaces representing surface roughness. The model resulted in a convolution integral for the scattered light intensity distribution, which yields an inverse specular problem after deconvolution. Thus, the shape of a reflector with a scattering surface may be computed using deconvolution, followed by solving the typical inverse problem of specular reflector design. We found that the presence of surface scattering resulted in a few percentage difference in terms of reflector radius, depending on the amount of scattering in the system.
Screening and diagnostic testing protocols for HIV and Syphilis infections in health care setting in Qatar: Evaluation and recommendations
Authors: Nasrallah Gheyath K. , Al-Buainain Raniya , Younes Nadin , Dargham Soha R. , Al-Sadeq Duaa W. , Elhassan Mohamed , Al-Shaar Ibrahim , Yassine Hadi M. , Abu-Raddad Laith J. , Emara Mohamed M. , Ismail Ahmed 
Published: February 2023

Background
HIV and Syphilis are common STIs, which have become a concern and burden on healthcare systems, as many infections go untreated and lead to potentially serious complications. HIV is usually diagnosed with Western blot, PCR, and p24 antigen testing. Whereas, Syphilis is mainly diagnosed through clinical findings and serologic testing. The Medical Commission Department (MC) under MOPH is responsible for screening all newcomers to Qatar, aiming to keep the country free from serious infectious diseases.

Objective
We aimed to evaluate the diagnostic efficiency of the protocols used in the MC for screening HIV and Syphilis infections.

Methods
We conducted a retrospective study of samples analyzed by 4th Generation ARCHITECT® HIV Ag/Ab Combo and Rapid Plasma Reagin (RPR) between January to December 2019. ARCHITECT® HIV Ag/Ab Combo positive samples were confirmed by INNO-LIA™ HIVI/II and RT-PCR. RPR-reactive samples were confirmed by ARCHITECT® Syphilis Treponema pallidium Antibody (Syphilis TPA) assay.

Results
For HIV, data were collected from 585,587 individuals, of which 595 (0.1%) were positive by the ARCHITECT® HIV Ag/Ab Combo (Analyzer A). When all initially positive sera were re-tested on newly collected blood samples using different ARCHITECT® HIV Ag/Ab Combo analyzer (analyzer B), 99.8% (594/595) of samples were also positive, suggesting high reproducibility. The positive predictive value (PPV) between ARCHITECT® HIV Ag/Ab Combo and the INNO-LIA™ HIVI/II confirmatory assay was 31.8%. The PPV between ARCHITECT® HIV Ag/Ab Combo and HIV-PCR assay was 26.8%. Retrospective data for Syphilis were collected from a total of 97,298 individuals who visited the MC, of which 198 (0.20%) were initially positive by RPR. The PPV between RPR and Syphilis TPA confirmatory assay was 36.6%.

Conclusion
Despite the high rate of false positivity using ARCHITECT® HIV Ag/Ab Combo and RPR screening assays, both assays have proven to be highly effective as screening testing methods.
El Tiempo entre Costuras: Música de entretelas
Authors: Álvarez García Marta 
Published: February 2023

Las series son actualmente las producciones audiovisuales más consumidas, con una oferta cada vez más amplia y unos estándares de calidad en constante ascenso. En el ámbito español también se está experimentando este proceso y ya son muchos los títulos que han sido premiados tanto a nivel de audiencia como de la crítica especializada. El Tiempo entre Costuras, miniserie emitida en Antena3 entre 2013 y 2014 e inspirada en la novela homónima de María Dueñas (2009), es un buen ejemplo.
A través del análisis de su Banda Sonora Musical, compuesta por César Benito, se muestran las particularidades de cuatro de los temas más relevantes, su relación con la imagen, con la protagonista y su identificación con un elemento clave en la trama: la máquina de coser. Además, se extraen algunas de las características generales de la música que favorecieron su conexión y cercanía con el espectador, motivo por el cual, junto con su idoneidad estilística y narrativa, contribuyó de forma determinante al éxito de la miniserie.
Henki Kleber on Bow and Strings
Authors: Purwandi Edeliya Relanika 
Published: February 2023

Abstract
This article tries to analyse the representation of postcolonialism in Indonesian children's literary works in the form of a novel entitled Komponis Kecil by Soesilo Toer. Komponis Kecil tells a story about the friendship of two figures namely Henki and Meneer Kleber. Meneer Kleber taught Henki to play a violin which at that time was considered as a musical instrument of the European or upper-class society, which later influenced Henki in the process of finding his true identity. This article uses qualitative social research methods with Komponis Kecil novel as the main research object. This article analyses the postcolonial concepts of otherness, mimicry and hybridity under the concept of orientalism. This article will use inductive analysis as data analysis method and hermeneutics as a methodology for interpreting texts; with the main narrative theme of Henki's hybridity which makes it unable to separate his European influences. This article also examines the expression of postcolonialism which also signifies the emergence of the social realism genre in Komponis Kecil.
Spatial variability of soil fertility under agroforestry system and native forest in eastern Amazonia, Brazil
Authors: Santos Cassio Rafael Costa dos , Matsunaga Augusto Takayuki , Costa Luiz Rodolfo Reis , Santos Mario Lima dos , Brasil Neto Alberto Bentes , Rodrigues Richard Pinheiro , Maciel Maria de Nazaré Martins , Melo Vânia Silva de 
Published: February 2023

The usage of spatial tools might be helpful in the optimization of decision-making regarding soil management, with technologies that assist in the interpretation of information related to soil fertility. Therefore, the present study evaluated the spatial variability of chemical attributes of the soil under an agroforestry system compared to a native forest in the municipality of Tomé-açu, Eastern Amazon, Brazil. Soil samples were performed at 36 points arranged in a 55 x 55 m grid. The soils were prepared and submitted to analysis in order to determine pH in H2O, exchangeable calcium, magnesium, potassium and aluminium, available phosphorus, potential acidity, organic matter, bases saturation and aluminium saturation. For each soil attribute, the spherical, gaussian and exponential models were adjusted. After the semivariograms fitting, data interpolation for assessment of spatial variability of the variables was performed through ordinary kriging. The spherical and gaussian models were the most efficient models in estimation of soil attributes spatial variability, in most cases. Most of variables presented a regular spatial variability in their respective kriging maps, with some exceptions. In general, the kriging maps can be used, and we can take them as logistical maps for management and intervention practices in order to improve the soil fertility in the study areas. The results principal components indicate the need for integrated management of soil chemical attributes, with localized application of acidity correctors, fertilizers and other types of incomes, using the spatial variability of these fertility variables.
Vergleich und Modell-Transformation zwischen Module Type Package und einem Fähigkeitsmodell
Authors: Köcher Aljosha , Beers Lasse , Fay Alexander 
Published: February 2023

Zusammenfassung
Sowohl in der Prozess- als auch in der Fertigungsautomatisierung werden Ansätze gesucht, um neue Funktionalitäten effizient in Anlagen zu integrieren. Mit dem Module Type Package auf der einen und Fähigkeitsmodellen auf der anderen Seite nutzen beide Domänen jedoch inkompatible Modelle. In diesem Beitrag werden ein Vergleich der beiden Modellierungsansätze sowie eine automatisierte Transformation vorgestellt. Diese Transformation erlaubt es, Module Type Packages in ein semantisches Fähigkeitsmodell zu konvertieren. Somit werden die bisher inkompatiblen Modelle zusammengeführt und gemeinsam in Produktionsprozessen nutzbar.
Simultaneous Inversion of the Space-Dependent Source Term and the Initial Value in a Time-Fractional Diffusion Equation
Authors: Yu Shuang , Wang Zewen , Yang Hongqi 
Published: February 2023

Abstract
The inverse problem for simultaneously identifying the space-dependent source term and the initial value in a time-fractional diffusion equation is studied in this paper.
The simultaneous inversion is formulated into a system of two operator equations based on the Fourier method to the time-fractional diffusion equation.
Under some suitable assumptions, the conditional stability of simultaneous inversion solutions is established, and the exponential Tikhonov regularization method is proposed to obtain the good approximations of simultaneous inversion solutions.
Then the convergence estimations of inversion solutions are presented for a priori and a posteriori selections of regularization parameters.
Finally, numerical experiments are conducted to illustrate effectiveness of the proposed method.
Multi-source perceptual blind compensation inspection method for substation based on equipment’s visual blind area identification and saliency detection
Authors: Xie Zhigang , Su Huatang , Li Xiang , Yang Ke , Li Rui , Yang Jing 
Published: February 2023

Abstract
In order to expand the detection range and ensure the operation stability, the substation multi-source perception blind compensation detection method based on equipment visual blind area recognition and significance detection is studied. Acoustic sensors are used to collect acoustic signals from visual blind areas of equipment. The characteristics of noise signal are identified by wavelet analysis and noise reduction. The supercomplex Fourier transform model was used to extract the important region in the device image, and the texture features of the region were detected by Gabor filter. The blind compensation detection feature vector is formed by integrating two multi-source sensing features. The detection model of support vector machine is input to complete the blind compensation detection of the substation. The experimental results show that the proposed method is effective for the sound signal feature recognition in the visual blind area and the texture feature detection in the significant area of the device image. The different operating states of each equipment detected by the multi-source sensing feature vector are more accurate, which can realize the purpose of the multi-source sensing blind compensation check of the substation and ensure the safe and stable operation of the substation.
In vitro immunomodulatory activity study of Garcinia cowa Roxb. fraction
Authors: Dewi Irene Puspa , Wahyuni Fatma Sri , Aldi Yufri , Ismail Nor Hadiani , Dachriyanus  
Published: February 2023

Abstract

Objectives
The objective of this study is to determine the activity of Garcinia cowa Roxb. n-hexane, ethyl acetate, and butanol fractions as an immunomodulator in vitro and obtain the fraction that has the potential as an immunomodulator.

Methods
Raw 264.7 macrophages were used to asses G. cowa Roxb. immunomodulatory activity. The MTT assay was chosen to measure cell viability to evaluate the cytotoxic effect on cells. ELISA method was used to measure the concentration of Interleukin-6 (IL-6) and Tumor Necrosis Factor Alpha (TNF-α) secreted by cells after being treated with G. cowa Roxb. fraction. The neutral red uptake assay determined the effect of Garcinia cowa Roxb. on the phagocytic activity.

Results
After Raw 264.7 macrophages were given the Hexan fraction (Hex) at concentrations of 12.5 and 25 μg/mL, there was a decrease in the concentration of IL-6, TNF-α, and the phagocytosis index of cells. Administration of the Ethyl Acetate fraction (EtOAc) at concentrations of 12.5 and 25 μg/mL on cells caused a decrease in IL-6 and TNF-α levels but did not affect the phagocytosis index. There was an increase in the level of TNF-α and the phagocytosis index after being given the Butanol fraction (BuOH) with concentrations of 12.5 and 25 μg/mL but there was a slight decrease in the level of IL-6.

Conclusions
Both Hex and EtOAc fractions could suppress immune responses through decreasing IL-6, TNF-α, and slightly decreased phagocytic activity. BuOH fraction could stimulate immunomodulatory activities through enhanced TNF-α levels and phagocytic index, but less potent in enhancing IL-6 production. The BuOH fraction could be developed as an immunostimulant.
Very High Cycle Fatigue Behavior of Austenitic Stainless Steel in Annealed and Predeformed Condition
Authors: Sarkar Aritra , Klusak Jan , Kozáková Kamila , Christ H.-J. 
Published: February 2023

Abstract
Engineering components in service nowadays are designed for a large number of loading cycles (∼109), which makes fatigue behavior in this cycle range (designated as very high cycle fatigue [VHCF]) a topic of significant interest. The present study aims to investigate the high cycle fatigue (HCF) and VHCF behavior in type 304 stainless steel in two different conditions (annealed and with a martensite volume fraction of ∼45 %) through low cycle fatigue predeformation at a subzero temperature. A fatigue diagram in the form of an S–N curve extending up to the VHCF range is generated, which shows different HCF and VHCF behaviors for the annealed and predeformed conditions. Further tests are carried out at different R ratios to generate constant-life Haigh diagrams for both conditions. Although no failure is observed in the VHCF range under the annealed condition, an internal crack initiation mode is observed under the predeformed condition (Type II behavior), which is attributed to the enhanced notch sensitivity under VHCF cycling induced by a significant extent of strain-induced martensite formed during predeformation. To address the influence of varying load history under service, specimens were prefatigued in HCF, followed by VHCF cycling. The HCF–VHCF boundary is found to be significantly impacted by the prior HCF damage, with lowering of the fatigue limit under VHCF.