bluuebunny/crossref_metadata_2025_split huggingface dataset:
Integrative Data Mining Pipeline for Identification of a Protein- Based Prognostic Signature in Lung Squamous Cell Carcinoma
Authors: Lei Ming , Shi Qiong , Chen Nan , Li Zhenhui , Lin Yichen , Wei Ying , Li Zhai 
Published: March 2023

Abstract
The purpose of this study is to use an integrated data mining approach, in which multi-omics, clinical information, and image information are considered together, and to develop a new prognosis prediction model for Lung Squamous Cell Carcinoma (LUSC). We analyzed Reverse Phase Protein Array (RPPA) data of LUSC samples (n = 328) from The Cancer Genome Atlas cohort (TCGA). Univariate Cox regression analysis and the least absolute shrinkage and selection operator (LASSO) regression analysis followed by multivariate Cox analysis were performed to identify key protein candidates and constructed a robust multiprotein prognostic model on the training set. The optimal cut-off value was obtained by the receiver operating characteristic (ROC) curve, which was employed to divide patients into a high- and a low-risk group. The model was evaluated using multiple statistical methods, including principal components analysis (PCA), Kaplan-Meier survival analysis, independent prognostic analysis, ROC analysis, and immunohistochemistry (IHC) staining. The co-expression analysis and bioinformatics enrichment analysis of gene function was adapted to evaluate the prognostic effect and biological pathways of the model. Four-protein (Histone-H3, HSP27_pS82, CHK2, and PAXILLIN) prognostic signature was able to stratify patients into high- and low-risk groups with statistical significance. The signature estimates poor overall survival for high-risk patients in both training and testing sets. Histone-H3, HSP27_pS82, and CHK2 were found to be protective, while PAXILLIN was associated with poor prognosis. Univariate and multivariate Cox regression analysis showed that the risk model was an independent risk factor for overall survival (univariate: HR = 3.558, 95%CI = 2.451–5.169, p< 0.001, multivariate: HR = 2.515, 95%CI = 1.750–3.615, p < 0.001). The area under the curve (AUC) of the risk scores was 0.742. The correlation heatmap provided a landscape for 455 proteins. The gene set enrichment analysis (GSEA) results revealed that adhesion molecular and cancer pathways were enriched in the high-risk group and the cytochrome P450 pathway was enriched in the low-risk groups. Our finding discovered a set of novel 4-related prognostic signatures could serve as a sensitive independent prognostic factor for individualized survival predictions.
Integrated microbiome-metabolome reveals thiram aggravates tibial dyschondroplasia through disturbing sphingolipid metabolism
Authors: Liu Yingwei , Wu Shouyan , Liu Kai , Li Ying , Liao Jiaozhao , Pan Jiaqiang , Hu Lianmei , Iqbal Mujahid , Mehmood Khalidx , Tang Zhaoxin , Zhang Hui 
Published: March 2023

Abstract
As a pesticide, thiram is widely used in agriculture to eliminate pests, and it also causes great environmental pollution and poses a certain threat to animal health. It’s well known that the contamination of feed with thiram in the environment can lead to tibial chondrogenesis(TD) in chickens. The imbalance of intestinal flora and related metabolites is closely related to bone development. Unfortunately, the relationship between the intestinal flora of TD broilers and serum metabolites is unclear. Our results demonstrated that broilers exposed to thiram showed typical lameness and the white cartilage thrombus in the growth plate, accompanied by hepatotoxicity and intestinal injury. We found that the intestinal flora of TD group was out of balance, the diversity was significantly increased with Corynebacterium significantly enriched. Moreover, the metabolome results showed alterations in 10 serum metabolites, with Glucosylceramide being considerably up-regulated, resulting in sphingolipid metabolism problem, which is critical in the etiology of TD. The comprehensive correlation analysis showed the relationship between intestinal microflora and Sphingolipid metabolism in TD broilers. Thiram aggravates tibial chondrodysplasia by affecting the changes in the composition and structure of the intestinal microflora of broilers and the disorder of sphingomyelin metabolism. Collectively, these findings provide novel insight into the pathogenesis of TD from the perspective of thiram-induced gut microbiota and metabolic disorders.
Development of cardiometabolic risk factors following endocrine therapy in women with breast cancer
Authors: Rillamas-Sun Eileen , Kwan Marilyn L. , Iribarren Carlos , Cheng Richard , Neugebauer Romain , Rana Jamal S. , Nguyen-Huynh Mai , Shi Zaixing , Laurent Cecile A. , Lee Valerie S. , Roh Janise M. , Huang Yuhan , Shen Hanjie , Hershman Dawn L. , Kushi Lawrence H. , Greenlee Heather 
Published: March 2023

Abstract
Purpose: Studies comparing the effect of aromatase inhibitor (AI) and tamoxifen use on cardiovascular disease (CVD) risk factors in hormone-receptor positive breast cancer (BC) survivors report conflicting results. We examined associations of endocrine therapy use with incident diabetes, dyslipidemia, and hypertension.
Methods: The Pathways Heart Study examines cancer treatment exposures with CVD-related outcomes in Kaiser Permanente Northern California members with BC. Electronic health records provided sociodemographic and health characteristics, BC treatment, and CVD risk factor data. Hazard ratios (HR) and 95% confidence intervals (CI) of incident diabetes, dyslipidemia, and hypertension in hormone-receptor positive BC survivors using AIs or tamoxifen compared with survivors not using endocrine therapy were estimated using Cox proportional hazards regression models adjusted for known confounders.
Results: In 8,985 BC survivors, mean baseline age and follow-up time was 63.3 and 7.8 years, respectively; 83.6% were postmenopausal. By treatment, 77.0% used AIs, 19.6% used tamoxifen, and 16.0% used neither. Postmenopausal women who used tamoxifen had an increased rate (HR: 1.43, 95% CI: 1.06-1.92) of developing hypertension relative to those who did not use endocrine therapy. Tamoxifen use was not associated with incident diabetes, dyslipidemia, or hypertension in premenopausal BC survivors. Postmenopausal AI users had higher hazard rates of developing diabetes (HR: 1.37, 95% CI: 1.05-1.80), dyslipidemia (HR: 1.58, 95% CI: 1.29-1.92) and hypertension (HR: 1.50, 95% CI: 1.24-1.82) compared with non-endocrine therapy users.
Conclusion: Hormone-receptor positive BC survivors treated with AIs may have higher rates of developing diabetes, dyslipidemia, and hypertension over an average 7.8 years post-diagnosis.
Hydrothermal synthesis of binary disulfide Co x Cu 1-x S 2 as cathode material for high-performance lithium thermal battery
Authors: Chen Chao , Gu Chengyu , Yang Yuli , Liu Haiping , Bi Sifu , Cao Lixin 
Published: March 2023

Abstract
The hollow microsphere structure materials binary disulfide Co1-xCuxS2 (x = 0, 0.05, 0.10, 0.15, 0.20, 0.25) were synthesized by hydrothermal method with cobalt sulfate, copper sulfate, and sodium thiosulfate. The structure, morphology, and composition of Co1-xCuxS2 were characterized by X-ray diffraction (XRD), field emission scanning electron microscope (FESEM), and energy dispersive spectrometer (EDS). The influence of cobalt and copper content in Co1-xCuxS2 cathode material on the discharge performance of the cell was investigated. The experimental results show that the Co0.95Cu0.05S2 sample possesses the largest number of hollow microspheres, the internal resistance of the cell was lower and the specific capacity of the cell is higher. The results of constant current discharge showed that the optimal composition Co0.95Cu0.05S2 exhibits an ultra-high specific capacity of 531.8 mAh g-1 at 450℃ and the current density of 100 mA·cm-2 with the cut-off voltage of 1.5V. The best discharge temperature of Co0.95Cu0.05S2 was 450℃.
Nitrogen concentration, its uptake, use efficiency and yield of wheat (Triticum aestivum L.) As influenced by difference in rate of nitrogen application in Khumaltar, Nepal
Authors: Rawal Nabin , Vista Shree Prasad , Khadka Dinesh 
Published: March 2023

Abstract
Appropriate application of nitrogen (N) not only increases wheat production but also results in optimum N use efficiency (NUE). An experiment was conducted to determine N concentration, uptake, use efficiency and performance of wheat in response to N fertility levels at Khumaltar Lalitpur, Nepal during 2019/20 and 20020/21. The experiment involved 7 treatments; 6 levels of N (0, 50, 75, 100, 125, 150 kg ha− 1) with application of P2O5:K2O in 50:50 kg ha− 1 and zero plot (no fertilizer application) which was laid out in randomized complete block design and replicated three times. The varying N had significant influence on grain yield, N concentration, its uptake, NUE and economics of wheat production. Two years pooled grain yield showed that significantly (p ≤ 0.001) higher grain yield (6.52 Mg ha− 1) with B: C ratio of 2.6 was observed from application of 150 kg N ha− 1 which was at par with use of N @ 125 kg ha− 1 (6.32 Mg ha− 1). The two years mean N concentration in grain and straw of wheat were 13.98 and 3.49 g kg− 1, respectively. The data revealed that 4.9 Mg ha− 1 grain yield plus 5.56 Mg ha− 1 dry straw yield removed 81.7 kg N ha− 1. Likewise, N partition in wheat grain and straw were 75% and 25% respectively. The mean partial factor productivity, internal efficiency, physiological efficiency, recovery efficiency, and agronomic efficiency of N in wheat was found to be 61.8 kg kg− 1, 59.1 kg kg− 1, 53.1 kg kg− 1, 45.0%, and 22.5 kg kg− 1, respectively. N was limiting nutrient as added N fertilizer had considerable impact in wheat production and recommended to use 125 kg N ha− 1 under similar soil properties and ecology.
Pre-radiotherapy ctDNA liquid biopsy for risk stratification of oligometastatic non-small cell lung cancer
Authors: Chaudhuri Aadel , Semenkovich Nicholas , Samson Pamela , Badiyan Shahed , Vlacich Gregory , Stowe Hayley , Wang Yun , Star Rachel , Devarakonda Siddhartha , Govindan Ramaswamy , Waqar Saiama , Robinson Clifford , Pellini Bruna 
Published: March 2023

Abstract
The optimal treatment for patients with oligometastatic non-small cell lung cancer (NSCLC) remains unclear. Some patients with oligometastatic disease can experience prolonged remission after locally consolidative radiation therapy (RT), while others harbor micrometastatic disease (below current limits of detection by imaging) that may benefit from further prioritization of systemic therapy. To better risk-stratify this population and identify the patients most likely to benefit from locally consolidative radiation therapy, we performed a multi-institutional cohort study of patients with oligometastatic NSCLC undergoing liquid biopsy analysis of circulating tumor DNA (ctDNA).

Among this real-world cohort of 1,487 patients undergoing analysis (using the Tempus xF assay), a total of 1,880 ctDNA liquid biopsies along with paired clinical data were obtained across various timepoints. Approximately 20% (n=309) of patients had ctDNA obtained prior to RT and after their diagnosis of oligometastatic disease. Samples were de-identified and analyzed for mutational burden and variant frequencies of detectable deleterious (or likely deleterious) mutations in plasma.

Patients with undetectable ctDNA before RT had significantly improved progression-free survival and overall survival compared to patients with detectable ctDNA prior to RT. In patients that received RT, 598 pathogenic (or likely deleterious) variants were identified. ctDNA mutational burden pre-RT and ctDNA maximum variant allele frequency (VAF) pre-RT were both significantly inversely correlated with both progression-free (P = 0.0031 for mutational burden, P = 0.0084 for maximum VAF) and overall survival (P = 0.045 for mutational burden, P = 0.0073 for maximum VAF). Patients without detectable ctDNA prior to RT had significantly improved progression-free survival (P = 0.004) and overall survival (P = 0.03) compared to patients with detectable ctDNA prior to RT.

These data suggest that in patients with oligometastatic NSCLC, pre-radiotherapy ctDNA analysis can potentially identify the patients most likely to benefit from locally consolidative RT and experience prolonged progression-free and overall survival. Similarly, ctDNA may be useful to identify those patients with undiagnosed micrometastatic disease, in whom it may be appropriate to prioritize systemic therapy.
Biodynamic Substrate for the Optimization of Biomass and Constitution of Metabolites of Plectranthus Ornatus
Authors: Suárez-Barreiro jovanna , Romero María , Velásquez Camilo , Tobon Felipe , Leguizamón Adriana 
Published: March 2023

Abstract

ThePlectranthus ornatusCodd. (also known as acetaminofem and boldo) has been found to have various pharmaceutical uses, including insecticidal properties. The metabolite composition of P. ornatus varies depending on soil and climatic conditions. The objective of this study was to optimize the growth and metabolite composition of P. ornatus (N = 72) through biodynamic substrate cultivation in Machetá-Cundinamarca, Colombia, located at 1850 masl, over a period of 60 days. Four different substrates were tested: sandy, vermicompost, horse manure, and biodynamic substrate, with the aim of identifying significant differences that would indicate optimization.The study evaluated root growth, plant material growth, and relative metabolite amounts (%) present in an ethanolic extract obtained under the same conditions. Additionally, the volatile fraction was identified using HS-SPME/GC-MS, and soil analysis was performed. The results showed that the plants grown in the biodynamic substrate had the highest growth in plant material and root (p < 0.05), while the plants grown in the sandy substrate had the highest concentration of volatile fraction in the extract. Furthermore, the plants grown in the biodynamic substrate exhibited greater vitality, and the physicochemical/microbiological composition of this substrate at the end of the trial showed a higher concentration of nutrients required for aromatics cultivation and a higher concentration of nitrogen-fixing bacteria.In conclusion, this study suggests that in the long term, the biodynamic substrate may be more efficient in obtaining metabolites of pharmaceutical interest, and a combination with sandy substrate should be considered for optimal results.
Evolution of periodic wave and dromion-like structure solutions in the variable-coefficients coupled high-order complex Ginzburg-Landau system
Authors: Yan Yuanyuan , Liu Wenjun , Wang Haotian , Liu Xiaoyan , Meng Xiankui , Yang Hujiang , Tian Ye 
Published: March 2023

Abstract
In this paper, the propagation characteristics of an ultrashort pulse in the birefringent fiber expressed by the variable-coefficients coupled high-order complex Ginzburg-Landau (HCGL) equations are analyzed. Firstly, the one-soliton solution of the coupled HCGL equations is obtained by the asymmetric method. And this is the first time that the coupled Ginzburg-Landau (GL) equations are successfully solved by the asymmetric method, which further expands the scope of application of the asymmetric method. Then, the evolution process and dynamic characteristics of special types of solitons such as the periodic wave and dromion-like structure in the variable-coefficients coupled HCGL system are discussed by examples. Furthermore, the combination of traditional bright soliton and dromon-like structure is constructed for the first time. These works are expected to promote the research on multi-soliton solutions in HCGL systems, which is still an unsolved problem, and are likely to contribute some theoretical guidance to the optimization of Wavelength Division Multiplexing (WDM) systems and the preparation of special types of soliton pairs in fiber lasers, so as to promote the development of optical soliton research and elevate information transmission rate in optical communication systems.
Patients, payers and developers of Orphan Medicinal Products: lessons learned from 10 years’ multi-stakeholder dialogue on improving access in Europe via MoCA
Authors: Cavaller-Bellaubi Maria , Hughes-Wilson Wills , Kubinová Šárka , De Casteele Marc Van , Lente Evert Jan Van , Degortes Emanuele , Pontén Johan , Eichler Hans-Georg , Cam Yann Le , Boselli Simone , Bucsics Anna 
Published: March 2023

Abstract
Background: The Mechanism of Coordinated Access to orphan medicinal products (MoCA) was established in 2013 with the intention of developing a coordinated mechanism between volunteering EU stakeholders and developers of Orphan Medicinal Products (OMPs) to support the exchange of information aimed at enabling informed decisions on pricing and reimbursement at Member State level and to evaluate the value of an OMP based on a Transparent Value Framework. The objective of the collaborative approach was to support more equitable access to authorised therapies for people living with rare diseases, rational prices for payers and more predictable market conditions for OMP developers. Over the past 10 years, the MoCA has conducted a series of pilot projects, examining a variety of different products and technologies at different stages of development; and with contributions from a variety of patient representatives, participation from EU payers from a range of Member States; and, recently, with EUnetHTA members and the EMA participating in the meetings as observers.
Results: 10 years on from the establishment of the MoCA, the European landscape has significantly evolved, not only in the field of drug development with increasingly transformative therapies based on novel technologies, but also in terms of larger numbers of approved treatments, increased budget impact and the resulting associated uncertainties; as well as in terms of stakeholder collaboration and interactions. The value of early dialogue with OMP developers, including the EU payer community via their national decision-making authorities, is a key element within this early interaction and contributes to identifying, managing and reducing uncertainties allowing a prospectively planned approach earlier in development and, consequently, to support more timely, sustainable and equitable access to new OMPs, particularly where there is a high unmet medical need.
Conclusions: The voluntary, informal nature of the MoCA interactions creates a flexible framework for non-binding dialogue. A forum for such interactions is needed to achieve the aims of the MoCA and to support both healthcare systems in planning as well as to underpin timely, equitable and sustainable access for patients with rare diseases to new therapies within the EU.
Formulated algae-based food with low polyphenol content and its effect on the feeding preference of juvenile blue abalone Haliotis fulgens
Authors: Villa-Arce Miguel Ángel , Muñoz-Ochoa Mauricio , Hernández-Carmona Gustavo , Mendoza-Cruz Margarita , Godínez-Pérez Carlos Alonso , Vélez-Arellano Nurenskaya 
Published: March 2023

Abstract
The objective of this study was to evaluate the effect of polyphenol reduction in a food formulated with Eisenia arborea and its effect on the feeding preference of the abalone Haliotis fulgens through multiple selection tests. Two foods were formulated: one without polyphenol reduction (EA01) and one with polyphenol reduction (EA02). Rehydrated E. arborea (ER03) was used as the control food. The polyphenol content was quantified in EA01 and EA02, and the stability and hardness of all three foods were measured at 24 h. Food preference was evaluated through attraction and consumption tests on day 1, 6, and 12. The polyphenol concentration was reduced by 41% in EA02 (13.9 mg GAE/g) compared to that of EA01 (33.3 mg GAE/g). Both formulated foods showed 88% stability and hardness values > 680 g cm− 2, which were greater than those of the control (ER03, 66% and 285 g cm− 2, respectively). Abalone were more attracted to EA02 and ER03 on day 1, 6, and 12 than to ER03 on the same days. A similar trend was observed with consumption. EA02 and ER03 were the most consumed foods (> 6 g/day) throughout the experiment, and no significant differences in consumption were observed between these foods. On the other hand, juvenile H. fulgens showed a greater attraction to and consumption of EA02 (reduced polyphenol content) than EA01 (no reduction in polyphenol content). This allows us to conclude that EA02 can replace rehydrated algae as a suitable food source for juvenile H. fulgens.
Twin primes as a consequence of Goldbach's conjecture
Authors: Nunez Hector 
Published: March 2023

Abstract
The binary Goldbach conjecture asserts that every even integer greater than 2 can be expressed as the sum of two primes. In addition, the ternary Goldbach conjecture states that every odd integer greater than 5 is the sum of three primes. Both conjectures originated in an exchange of letters between Leonhard Euler and Christian Goldbach in 1742. We claim that both Goldbach problems are valid with multiple partitions for every integer greater than 12. We approached the binary Goldbach problem by the second principle of mathematical induction. Based on the inductive hypothesis, we found that every even integer within a finite interval can be expressed as the difference between two primes. This fact allowed us to tackle the inductive step advantageously. Once both Goldbach problems were proved, we were empowered to claim that every even integer can be written infinitely many ways, as the difference between two primes, where the twin-prime conjecture is a particular case.
MSC Classification: 11P32 , 11N05
Dissimilar Joint Welding Through Friction Stir Techniques: Mechanical and Microstructural Properties of AA2198-T8 &amp; AA2024-T3
Authors: Samir Ahmed Anwar , Jalal Shawnim R , Mulapeer Mohammedtaher M 
Published: March 2023

Abstract
Friction stir welding is used to evaluate the metallurgical and material characteristics of AA2198-T8 and AA2024-T3 welds. One of the most often utilised high-strength aluminium alloys in the aerospace industry is AA2024-Tx. The latest generation of aluminium lithium alloys, AA2198-T8, just replaced AA2024-T3. Yet, sound weld methods with the highest mechanical qualities have yet to be adequately characterised. These two materials were selected and joined through two techniques. The first is a single side friction stir weld, while the second is a double side friction stir weld. This technique was used to create specimens by holding them side by side. After the stirring, the hybrid joint was created by taking spindle speeds of 580 RPM and travel speeds of 36, 76, 102, 146, and 216 mm/min into account. The tool angle and dwell duration evaluated in this study were 2o and 20 seconds, respectively. Fabricated samples were then sectioned in accordance with industry standards. Tests to determine factors like as tensile strength, impact load, and microstructures at various temperature zones. The results show that the maximum joint efficiency for a double-side friction stir welded connection at 102 mm/min was 96%.