bluuebunny/crossref_metadata_2025_split huggingface dataset:
Effects of dietary chlorogenic acid on ileal intestinal morphology, barrier function, immune factors and gut microbiota of broilers under high stocking density stress
Authors: Li Yu-Qian , Zhang Yi , Bai Dong-Ying , Liu Yan-Hao , He Xiang-Long , Ito Koichi , Liu Ke-Xin , Tan Hai-Qiu , Zhen Wen-Rui , Zhang Cai , Zhang Bing-Kun , Ma Yan-Bo 
Published: April 2023

Aims: The purpose of this research was to assess the effect of chlorogenic acid (CGA) in the diet on ileac structure, barrier function, immunological state, and microbial profile of broiler chickens in a high stocking density (HD) environment.Methods: Four hundred and seventy-six male AA broiler chickens were randomly split into four groups, two with a normal stocking density (ND) of fourteen birds per m2 and two with a high stocking density of twenty-two birds per m2. Each of the treatments consisted of five replicates. One of the two ND and HD groups received the usual feed, while the other two were given at 1.5 g/kg CGA as part of their dietary regimen.Results: The ND CGA group showed a greater increase in villus height and villus height/crypt depth compared to the ND group at 35 and 42 days. The HD group experienced a greater elevation in villus height due to CGA supplementation than the HD group across days 28, 35, and 42. At day 42, the HD group saw a decline in OCLN and ZO-1 mRNA expression in the ileum, but CGA was able to restore them. The HD group experienced a greater rise in OCLN mRNA than the control HD group when supplemented with CGA. The expression of TNF-α, IL-1β, and IL-6 in the ileum was higher in the HD group, and CGA supplementation enhanced this effect. The HD group experienced a greater rise in IL-10 mRNA expression than the control group following the administration of CGA. The HD group showed reduced alpha diversity and an increase in detrimental microbes such as Turicibacter and Shigella in the gut compared to the ND group, while the HD CGA group saw a reduction in Turicibacter, Shigella, and other harmful microbes. These findings reveal that HD stress suppressed the growth of ileac villi, decreased the expression of tight-junction genes, amplified the expression of inflammatory genes, and disturbed the gut microbiota, ultimately leading to increased intestinal permeability.Conclusion: We conclude that when chickens are given dietary CGA, the disruption of the ileac barrier and increased oxidative damage and inflammation due to HD stress are reduced, which increases ileac integrity and the presence of beneficial intestinal bacteria.
Self-supervised maize kernel classification and segmentation for embryo identification
Authors: Dong David , Nagasubramanian Koushik , Wang Ruidong , Frei Ursula K. , Jubery Talukder Z. , Lübberstedt Thomas , Ganapathysubramanian Baskar 
Published: April 2023

IntroductionComputer vision and deep learning (DL) techniques have succeeded in a wide range of diverse fields. Recently, these techniques have been successfully deployed in plant science applications to address food security, productivity, and environmental sustainability problems for a growing global population. However, training these DL models often necessitates the large-scale manual annotation of data which frequently becomes a tedious and time-and-resource- intensive process. Recent advances in self-supervised learning (SSL) methods have proven instrumental in overcoming these obstacles, using purely unlabeled datasets to pre-train DL models.MethodsHere, we implement the popular self-supervised contrastive learning methods of NNCLR Nearest neighbor Contrastive Learning of visual Representations) and SimCLR (Simple framework for Contrastive Learning of visual Representations) for the classification of spatial orientation and segmentation of embryos of maize kernels. Maize kernels are imaged using a commercial high-throughput imaging system. This image data is often used in multiple downstream applications across both production and breeding applications, for instance, sorting for oil content based on segmenting and quantifying the scutellum’s size and for classifying haploid and diploid kernels.Results and discussionWe show that in both classification and segmentation problems, SSL techniques outperform their purely supervised transfer learning-based counterparts and are significantly more annotation efficient. Additionally, we show that a single SSL pre-trained model can be efficiently finetuned for both classification and segmentation, indicating good transferability across multiple downstream applications. Segmentation models with SSL-pretrained backbones produce DICE similarity coefficients of 0.81, higher than the 0.78 and 0.73 of those with ImageNet-pretrained and randomly initialized backbones, respectively. We observe that finetuning classification and segmentation models on as little as 1% annotation produces competitive results. These results show SSL provides a meaningful step forward in data efficiency with agricultural deep learning and computer vision.
Interplay of silymarin and clove fruit extract effectively enhances cadmium stress tolerance in wheat (Triticum aestivum)
Authors: El-Sappah Ahmed H. , Metwally Mohamed A. S. , Rady Mostafa M. , Ali Hayssam M. , Wang Linghui , Maitra Pulak , Ihtisham Muhammad , Yan Kuan , Zhao Xin , Li Jia , Desoky El-Sayed M. 
Published: April 2023

IntroductionOsmoprotectant supplementation can be used as a useful approach to enhance plant stress tolerance. However, the effect of silymarin and clove fruit extract (CFE) on wheat plants grown under cadmium (Cd) stress has not been studied.MethodsWheat seeds were planted in plastic pots filled with ions-free sand. A ½-strength Hoagland’s nutrient solution was used for irrigation. Pots were treated with eight treatments thirteen days after sowing: 1) Control, 2) 0.5 mM silymarin foliar application [silymarin], 3) 2% CFE foliar application [CFE], 4) CFE enriched with silymarin (0.24 g silymarin L-1 of CFE) [CFE-silymarin], 5) Watering wheat seedlings with a nutritious solution of 2 mM Cd [Cd]. 6) Cadmium + silymarin, 7) Cadmium + CFE, and 8) Cadmium + CFE-silymarin. The experimental design was a completely randomized design with nine replicates.Results and discussionThe Cd stress decreased grain yield, shoot dry weight, leaf area, carotenoids, chlorophylls, stomatal conductance, net photosynthetic rate, transpiration rate, membrane stability index, nitrogen, phosphorus, and potassium content by 66.9, 60.6, 56.7, 23.8, 33.5, 48.1, 41.2, 48.7, 42.5, 24.1, 39.9, and 24.1%, respectively. On the other hand, Cd has an Application of CFE, silymarin, or CEF-silymarin for wheat plants grown under Cd stress, significantly improved all investigated biochemical, morphological, and physiological variables and enhanced the antioxidant enzyme activities. Applying CFE and/or silymarin enhanced plant tolerance to Cd stress more efficiently. Our findings suggest using CFE-silymarin as a meaningful biostimulator for wheat plants to increase wheat plants’ tolerance to Cd stressviaenhancing various metabolic and physiological processes.
Why time flies? The role of immersion in short video usage behavior
Authors: Yan Yurong , He Yingfei , Li Longfei 
Published: April 2023

BackgroundShort videos are becoming increasingly popular globally, and users are devoting more time to viewing them. However, few studies have examined the characteristics of short video content and the technical features that are related to media use. The present study developed a model to explore the influence of technological affordances on short video usage and considered innovation in terms of format, setting, and content.MethodA total of 496 viewers of short videos from China were surveyed. The participants completed 31 Likert-scale items. The study used maximum likelihood estimation modeling.ResultsThe results revealed that perceived novelty and content value (entertainment and irritation) affected immersion positively, consequently influencing intentions for reuse and recommendation. The ease of navigating an application, narrative structure, and information value had no significant effect on immersion.ConclusionThese findings have confirmed that perceived situation affordance and the affective affordance of short videos in digital environments that are managed by intelligent algorithms. It is necessary to analyze the potential impact of different affordances specifically.
Different facets of COVID-19-related stress in relation to emotional well-being, life satisfaction, and sleep quality
Authors: Saalwirth Christina , Leipold Bernhard 
Published: April 2023

IntroductionAs the COVID-19 pandemic has shown, it is of great importance to investigate how people can maintain their mental health during chronically stressful times. This study therefore investigated which facets of COVID-19-related stress (Fear of COVID-19, financial worries, and social isolation) impacted people the most during a third COVID-19 infection wave from March until May 2021 and how these facets relate to well-being (emotional well-being and life satisfaction) and sleep quality.MethodsA study sample of 480 German participants (Mage = 43,SDage = 13.7, 20–69 years, 50.8% female) completed a cross-sectional online questionnaire.ResultsAs predicted, social isolation was reported most often, followed by fear of COVID-19 and financial worries. In accordance with our expectations more social isolation and financial worries predicted lower emotional well-being and sleep quality. In contrast to our hypothesis, fear of COVID-19 only predicted emotional well-being and not sleep quality. Life satisfaction was solely predicted by financial worries and not by social isolation and fear of COVID-19, which only partly confirmed our hypotheses. These associations remained stable after controlling for age, gender, household income, and living alone.DiscussionFinancial worries, although reported the least often, were the strongest and most stable predictor for emotional well-being, sleep quality, and life satisfaction. Implications for future research and practice are discussed.
Clinical Perception and Treatment Options for Behavioral and Psychological Symptoms of Dementia (BPSD) in Italy
Authors: D'Antonio Fabrizia , Tremolizzo Lucio , Zuffi Marta , Pomati Simone , Farina Elisabetta ,   Sindem BPSD Study Group
Published: April 2023

BackgroundBehavioral and psychological symptoms of dementia (BPSD) have a high prevalence, and their presence is associated with a severe impact in terms of social costs. However, dedicated clinical tools or biomarkers to detect these symptoms are lacking. Thus, BPSD management in clinical settings is challenging. The aim of this study was to investigate the perception and the treatment strategies for BPSD in Italian centers working in the dementia field.MethodsA multicenter, national survey was developed by BPSD Study Group of the Italian Neurological Society for Dementia (SINDEM). The survey consisted of a semi-structured questionnaire that was e-mailed to SINDEM members, dementia centers part of the national network of memory clinics (Centers for Cognitive Deterioration and Dementia [CDCD]), and clinicians working in dementia care settings. The questions were focused on (1) perceived global frequency and relevance of BPSD; (2) tools used to assess BPSD; (3) pharmacological treatment for psychosis, apathy, agitation, aggression, depression, anxiety, sleep, and nutrition disturbances; (4) non-pharmacological treatments; (5) drugs side effects.ResultsOne-hundred and thirty-six clinicians participated in this study. Seventy-nine participants worked in a CDCD and 57 in other settings. The perceived frequency of BPSD was 74%. BPSD are detected by means of a clinical assessment for 96.3% or a caregiver interview for 97%. For psychosis treatment the first choice was atypical antipsychotics (83.3%), followed by typical antipsychotic (8.9%) and antidepressants (4.8%). For agitation, atypical antipsychotics were the first-choice treatment in 64% of cases and antidepressants in 16.1%. For aggression, the most used drugs were atypical antipsychotics (82.9%). For anxiety, 55.2% use antidepressants, 17.9% use atypical antipsychotics, and 16.9% use benzodiazepines. Interestingly, most of the centers apply non-pharmacological treatments for BPSD. Some differences emerged comparing the responses from CDCD and other care settings.ConclusionThe survey results revealed many differences in BPSD perception, treatment options, and observed side effect according to the clinical setting. This variability can be explained by the absence of clear guidelines, by differences in patients' characteristics, and by clinical practice based on subjective experience. These results suggest that producing guidelines for the pharmacological treatment of BPSD is a major need.
Perspectives on the therapeutic potential of MDMA: A nation-wide exploratory survey among substance users
Authors: Jones Jennifer L. 
Published: April 2023

BackgroundAlcohol and other substance use disorders are commonly associated with post-traumatic stress disorder (PTSD), and the presence of these comorbidities is associated with worse treatment outcomes. Additionally, disparities in substance and PTSD prevalence have been associated with minority races and ethnicities, and minorities have been shown to be less likely to engage in treatment. Psychedelic-assisted treatments, including 3,4-methylenedioxymethamphetamine (MDMA), have shown preliminary trans-diagnostic effectiveness, however it is unknown how individuals with substance use disorders view the therapeutic potential of MDMA therapy. Previous studies have also shown that minority races and ethnicities are under-represented in the MDMA trials, leading to concerns about inequitable access to clinical treatment.MethodsTo explore demographic characteristics related to patient-level perspectives on the therapeutic potential of MDMA-assisted therapy, this study describes data from a nationwide, cross-sectional survey of 918 individuals self-reporting criteria consistent with alcohol or substance use disorders.ResultsOverall, a majority of individuals reported support for medical research of MDMA (68.1%), belief that MDMA-assisted therapy might be a useful treatment (70.1%), and willingness to try MDMA-assisted therapy if it were determined to be an appropriate treatment for them (58.8%). No race or ethnicity differences were found in support for further research or belief in effectiveness, however there were small disparities in terms of willingness to try MDMA-assisted therapy and concerns related to use of this treatment approach.ConclusionThese results provide insights and future directions as the field of psychedelic-assisted therapy seeks to provide equitable access to clinical care and to diversify research participation.
Age-related changes in prepulse inhibition of the startle response
Authors: de Oliveira Yasmin Guedes , Poltronieri Bruno Costa , Woodruff Erica , da Costa Brunno Freitas , Panizzutti Rogerio Arena 
Published: April 2023

IntroductionAcoustic prepulse inhibition of the startle response (PPI) is a phenomenon characterized by the reduction in the startle reflex caused by the presence of weak and brief stimulus before an intense and sudden stimulus (pulse). These phenomena can be observed in several species, but in humans it is commonly measured by the eyeblink using electromyography. PPI works as an operational measure of sensorimotor gating, which is the ability to suppress motor responses for sensory stimulus. Healthy aging is marked by several changes in neural processing, like inhibitory functioning decline. In this line, PPI measure can be a potential biomarker for changes related to the aging process.MethodsIn this research we aim to investigate if PPI is reduced with aging and if this reduction would be associated with cognitive functioning of older adults. To this aim, we compared PPI levels of older adults (over 60 years old) with PPI levels of young adults (from 18 to 28 years old).ResultsWith that, we found, significantly lower PPI level (F[1,25] = 7.44 p = 0.01) and lower startle amplitude startle amplitude: (U = 26.000 p = 0.001) in older adults than in young adults. However, we did not find differences in levels of habituation (T = −1.1 p = 0.28) and correlation between PPI and cognition within the sample of healthy older adults.DiscussionOur results demonstrate that aging is a factor that affects PPI and that it does not seem to predict cognition, however, future studies should explore the potential of using PPI for monitoring cognitive changes associated with techniques such as cognitive training.
Engaging older adults with a migration background to explore the usage of digital technologies in coping with dementia
Authors: van Leersum Catharina M. , Konrad Kornelia E. , Siebrand Egbert , Malik Zohrah B. , den Ouden Marjolein E. M. , Bults Marloes 
Published: April 2023

BackgroundCoping with dementia can imply particular challenges for people with a migration background due to diversity in their life course, personal characteristics, and living environment. Some of the services available for people with dementia include digital technologies for care, providing health services, and maintaining or increasing participation, independence, and safety. This study aimed to explore the role of digital technology in coping with dementia in the lives of older adults with a migration background, and the possibilities to engage and collaborate with older adults.MethodsThis study combined a qualitative interview-based approach with citizen science principles in the design and execution of a project studying the use of Anne4Care.Results and discussionParticipants valued that technology should provide health benefits and fit into aspects of their daily lives. Anne4Care was considered helpful in staying independent and connecting to loved ones in their country of birth. The participants needed to learn new competencies to work with the device, and not all had the material prerequisites, such as an internet connection. Still, this learning process was considered purposeful in their life, and the virtual assistant could be integrated into care and daily practices. The involvement of the older adults with dementia as co-researchers made them feel valuable and as equal partners during this research. An important prerequisite for the involvement of older adults with a migration background was existing relations with carers and care organizations.ConclusionDigital care technologies to cope with dementia can become a valuable part of care practices in the lives of older adults with a migration background. Involving older adults in the development of technology, acknowledging their expertise and needs, and working together in short iterations to adapt the technology for their specific needs and situations were experienced as valuable by the researchers, older adults, and care professionals.
A snap-shot of a diarrheal epidemic in Dhaka due to enterotoxigenic Escherichia coli and Vibrio cholerae O1 in 2022
Authors: Tauheed Imam , Ahmed Tasnuva , Akter Afroza , Firoj Md Golam , Ahmmed Faisal , Rahman Sadia Isfat Ara , Afrad Mokibul Hassan , Islam Md Nazmul , Rahman Aninda , Khan Ashraful Islam , Alam Baharul , Bhuiyan Taufiqur Rahman , Chowdhury Fahima , Qadri Firdausi 
Published: April 2023

BackgroundEnterotoxigenic Escherichia coli (ETEC) and Vibrio cholerae O1 are most common bacterial causes of diarrheal diseases in Bangladesh. This analysis projected distribution of ETEC and V. cholerae O1 among diarrheal patients of icddr,b, Dhaka hospital in two diarrheal peaks of 2022.MethodologyUnder the 2% systematic surveillance system, stool samples collected from diarrheal patients of icddr,b hospital were cultured and diagnostic testing was done for ETEC and V. cholerae O1. Comparison of positive cases was done between first peak (March–April) and second peak (October–November) in 2022.ResultsA total of 2,937 stool specimens were tested of which 12% were ETEC and 20% were V. cholerae O1. About 40% of the severe dehydration cases were infected with V. cholerae O1. Predominant ETEC enterotoxin type was ‘LT/ST’ (41%). The LT enterotoxin significantly increased from 13% to 28% in the second peak (p = 0.015). The predominant colonization factors (CFs) on ETEC were CS5 + CS6 (23%), followed by CS6 (15%). CF-positive isolates was significantly higher in the second peak (36%) than in the first peak (22%) (p = 0.043). Total 14% cases were co-infected with ETEC and V. cholerae O1. Significant differences in the distribution of enterotoxin types were observed (p = 0.029) among the co-infection cases.ConclusionChanging patterns of enterotoxin and CFs observed in ETEC pathogens should be taken into consideration for ETEC vaccine development. Considering cholera and ETEC biannual trends in causing diarrheal epidemics and outbreaks, emphasizes the need for thoughts on combination vaccine strategies for preventing acute watery diarrhea due to the two major bacterial pathogens.
Using augmented reality filters to display time-based visual cues
Authors: Stuart Jacob , Stephen Anita , Aul Karen , Bumbach Michael D. , Huffman Shari , Russo Brooke , Lok Benjamin 
Published: April 2023

Introduction: Healthcare education commonly uses practices like moulage to represent visual cues (e.g., symptoms). Unfortunately, current practices have limitations in accurately representing visual symptoms that develop over time. To address this challenge, we applied augmented reality (AR) filters to images displayed on computer screens to enable real-time interactive visualizations of symptom development. Additionally, this study explores the impact of object and filter fidelity on users’ perceptions of visual cues during training, providing evidence-based recommendations on the effective use of filters in healthcare education.Methods: We conducted a 2 × 2 within-subjects study that involved second-year nursing students (N = 55) from the University of Florida. The study manipulated two factors: filter fidelity and object fidelity. Filter fidelity was manipulated by applying either a filter based on a medical illustration image or a filter based on a real symptom image. Object fidelity was manipulated by overlaying the filter on either a medical manikin image or a real person image. To ensure that potential confounding variables such as lighting or 3D tracking did not affect the results, 101 images were pre-generated for each of the four conditions. These images mapped to the transparency levels of the filters, which ranged from 0 to 100. Participants interacted with the images on a computer screen using visual analog scales, manipulating the transparency of the symptoms until they identified changes occurring on the image and distinct symptom patterns. Participants also rated the severity and realism of each condition and provided feedback on how the filter and object fidelities impacted their perceptions.Results: We found evidence that object and filter fidelity impacted user perceptions of symptom realism and severity and even affected users’ abilities to identify the symptoms. This includes symptoms being seen as more realistic when overlaid on the real person, symptoms being identified at earlier stages of development when overlaid on the manikin, and symptoms being seen as most severe when the real-image filter was overlayed on the manikin.Conclusion: This work implemented a novel approach that uses AR filters to display visual cues that develop over time. Additionally, this work’s investigation into fidelity allows us to provide evidence-based recommendations on how and when AR filters can be effectively used in healthcare education.