bluuebunny/crossref_metadata_2025_split huggingface dataset:
Acute cold stress induces intestinal injury via CIRP-TLR4-IRE1 signaling pathway in pre-starter broilers: short communication
Authors: Wu Juanjuan , Li Guiyao , Guo Haoneng , Huang Bo , Li Guanhong , Dai Sifa 
Published: February 2023

Abstract
Background Cold stress is a common environmental stress in broiler chicks. Cold-inducible RNA-binding protein (CIRP) is a conserved cold shock protein that can regulate inflammatory response through Toll-like receptor 4 (TLR4). The mechanism that how CIRP involves in the regulation of cold stress in broilers remains unclear.
Methods and Results In this study, 360 7-day-old healthy male SZ901 chicks were selected and randomly allocated to 4 groups, and then subjected to acute cold exposure at the ambient temperature of 12 ± 1℃ for 0 h, 4 h, 8 h, and 12 h, respectively. After cold exposure, abdominal temperature, gene expression of CIRP-TLR4-IRE1 signaling pathway in ileum mucosa, and small intestinal structure were measured. The results showed that cold exposure decreased abdominal temperature, upregulated the gene expression of endoplasmic reticulum stress (ERS) markers IRE1, inflammatory factors IL-1β, IL-6, IL-10, TNF-α, and tight junction proteins ZO-1 and Occludin in ileum of chicks compared with the control group with no (0 h) cold exposure. Compared with the control group, a long time cold exposure upregulated the gene expression of CIRP, TLR4, GRP78, NF-κB in ileum mucosa, and decreased the villus height and V/C of small intestine.
Conclusions The above results suggest that acute cold stress induces endoplasmic reticulum stress via upregulating the gene expression of CIRP-TLR4-IRE1 signaling pathway, and results in the structural damage of chick intestine.
Application of hybrid BOPPPS teaching model in clinical internship in gynecology
Authors: Xu Zhengfen , Wang Xiaoxia , Yang Xiaodi , Che Xuan 
Published: February 2023

Abstract
Background The traditional gynecological teaching model is not conducive to the cultivation of interns’ clinical skills, thinking patterns and doctor-patient communication ability. This study is aim to explore the application effect of hybrid BOPPPS (Bridge-in, Objective, Pre-assessment, Participant learning, Post assessment, Summary) teaching model in a clinical internship in gynecology.
Methods This observational study was conducted among the final year undergraduate medical interns from September 2020 to June 2022 at Jiaxing Maternity and Child Health Care Hospital. The control group consisted 114 interns of grade 2017 who received the traditional teaching model. The experimental group consisted 121 interns of grade 2018 were introduced to the hybrid BOPPPS teaching model. interns’ final examination scores and teaching satisfaction were compared.
Results The final examination scores of interns in the experimental group were significantly higher than those of interns in the control group (P <0.05). In the experimental group, the final theoretical exam scores were significantly higher than the pre-assessment scores (P <0.01). Compared with the control group, 93.4% of interns in the experimental group thought the hybrid BOPPPS teaching model could help them to improve case analysis ability, and the difference is statistically significant (P < 0.05). 89.3% of interns in the experimental group supported the promotion and application of hybrid BOPPPS model in the practice of other disciplines.
Conclusion The hybrid BOPPPS teaching model helps to stimulate interns' learning interest and learning initiative, improve interns’ clinical skills, and have high interns’ satisfaction, which is worth of promotion and application in the practice of other disciplines.
Investigation of human hair keratin based nanofiberous scaffold for skin tissue engineering application
Authors: AADIL KESHAW Ram , Nathani Akash , Rajendran Archana , Sharma Chandra S. , Lenka Nibedita , Gupta Pratima 
Published: February 2023

Abstract
Keratin-based nanofibers were fabricated using the electrospinning technique and their potential as scaffolds for tissue engineering was investigated. Keratin, extracted from the human hair was blended with poly(vinyl alcohol) (PVA) in an aqueous medium. Morphological characterization of the fabricated PVA-keratin nanofiber (PK-NFs) random and aligned scaffolds was performed [1,2]using a scanning electron microscope (SEM) analysis. Functional groups and structural studies were done by infrared spectroscopy (FTIR) and X-ray diffraction (XRD) analysis. SEM study revealed the formation of uniform and randomly oriented nanofibers with an interconnected three-dimensional network structure. The mean diameter of the nanofibers ranged from 100 to 250 nm. FTIR study suggested that PVA interacted with keratin by hydrogen bonding. Moreover, the in-vitro cell culture study of PK-NFs scaffolds was tested on murine embryonic stem cells (ESCs), human keratinocytes (HaCaT) and dermal fibroblast (NHDF) cell lines. Results revealed that PK-NFs scaffolds were non-toxic and biocompatible by supporting the growth of murine ESCs, HaCaT and NHDF cells. Further, the immunocytochemical characterization revealed the successful adhesion and growth of ESCs, HaCaT and NHDF cells on PK-NFs scaffolds. Moreover, there was no noteworthy difference in terms of cell growth and viability irrespective of the random and aligned internal fibril arrangement of the PK-NFs scaffolds. The in-vitro cell culture study demonstrated the potential of PK-NFs as a scaffold for skin tissue engineering and it can also be a favorable material for different tissue engineering applications.
Does Cr2AlN Have the Highest Possible Superconducting Transition Temperature in the M2AX family?
Authors: Karaca E. , Byrne P. J. P. , Hasnip P. J. , Probert M. I. J. 
Published: February 2023

Abstract
We have developed a high-throughput computational method to predict the superconducting transition temperature in stable hexagonal M2AX phases and applied it to all the known possible choices for M (M: Sc, Ti, V, Cr, Mn, Fe, Y, Zr, Nb, Mo, Lu, Hf and Ta). We combine this with the best candidates for A (A: Al, Cu, Ge and Sn) and X (X: C and N) from our previous work, and predict Tc for 60 M2AX-phase materials, 53 of which have never been studied before. From all of these, we identify Cr2AlN as the best candidate for the highest Tc, and confirm its high Tc with more detailed density functional theory electron-phonon coupling calculations. Our detailed calculations predict Tc = 14.8 K for Cr2AlN, which is significantly higher than any Tc value known or predicted for any material in the M2AX family to date.
Sec1 Regulates Intestinal Mucosal Immunity in A Mouse Model of Inflammatory Bowel Disease
Authors: Cai Jing , Wu Hao , Wang Chenxing , Chen Yujiao , Zhang Dingli , Guan Shiwei , Fu Beilei , Jin Yingli , Cao Qian 
Published: February 2023

Abstract
Inflammatory bowel disease (IBD) is a common immune-mediated condition with its molecular pathogenesis remaining to be fully elucidated. This study aimed to deepen our understanding of the role of fut2 in human IBD, by studying a new surrogate gene sec1 in mice. CRISPR/Cas9 was used to prepare sec1 knockout (Sec1-/-) mice. IBD was induced in mice using 3% w/v dextran sulphate sodium. Small interfering RNA (siRNA) was employed to silence Sec1 in murine colon cancer cell lines CT26.WT and CMT93. IBD-related symptoms, colonic immune responses, proliferation and apoptosis of colon epithelial cells were assessed respectively to determine the role of Sec1 in mouse IBD. Impact of Sec1 on the expression of death receptor 5 (DR5) and other apoptosis-associated proteins were determined. Sec1 knockout was found to be associated with deterioration of IBD in mice and elevated immune responses in the colonic mucosa. Silencing Sec1 in CT26.WT and CMT93 cells led to less secretion of inflammatory cytokines IL-1β, IL-6 and TNF-α. CCK8 assay, flow cytometry and TUNEL detection suggested that Sec1 expression promoted the proliferation of colon epithelial cells, inhibited cell apoptosis, reduced cell arrest in G0/G1 phase and facilitated repair of inflammatory injury. Over-expression of DR5 and several apoptosis-related effector proteins was noticed in Sec1-/- mice and Sec1-silenced CT26.WT and CMT93 cells, supporting a suppressive role of Sec1 in cell apoptosis. Our results depicted important regulatory roles of Sec1 in mouse IBD, reflecting the importance of Fut2 in the pathogenesis of human IBD.
Chicken pituitary transcriptomic responses to acute heat stress
Authors: Pritchett Elizabeth M. , Goor Angelica Van , Schneider Blair K. , Young Meaghan , Lamont Susan J. , Schmidt Carl J. 
Published: February 2023

Abstract
Background
Poultry production is vulnerable to increasing temperatures in terms of animal welfare and in economic losses. With the predicted increase in global temperature and the number and severity of heat waves, it is important to understand how chickens raised for food respond to heat stress. This knowledge can be used to determine how to select chickens that are adapted to thermal challenge. As neuroendocrine organs, the hypothalamus and pituitary provide systemic regulation of the heat stress response.
Methods and Results
Here we report a transcriptome analysis of the pituitary response to acute heat stress. Chickens were stressed for 2 hours at 35˚C (HS) and transcriptomes compared with birds maintained in thermoneutral temperatures (25˚C).
Conclusions
The observations were evaluated in the context of ontology terms and pathways to describe the pituitaries response to heat stress. The pituitaries of heat stressed birds exhibited responses to hyperthermia through altered expression of genes coding for chaperones, cell cycle regulators, cholesterol synthesis, transcription factors, along with the secreted peptide hormones. prolactin, and proopiomelanocortin.
Experimental Study on Liquid Sloshing Characteristics in a Propellant Tank with Perforated Damping Structure on a Satellite
Authors: Qu Zhen , Li Yong , Liu Jintao , Cai Kun , Long Jun , Yao Can 
Published: February 2023

Abstract
The liquid in the satellite propellant tank is inevitably sloshing due to the vibration excitation of the rocket body. During the process of launch and satellite orbit change, the vibration excitation will be transmitted to the propellant tank. The resulting sloshing of liquid in the tank will generate additional sloshing force and moment applied on the satellite, which will have a serious impact on the satellite's attitude. In this paper, the sloshing performance of liquid in the large capacity propellant tank on the satellite is tested. The liquid sloshing tests were carried out on upper and lower tanks with a porous damping structure under different filling ratios, respectively. The mechanics data was collected by the sensor and analyzed using the classic pendulum model. Based on the test data and classic theory, the sloshing frequency, sloshing quality and sloshing damping parameters of upper and lower tanks were computed and compared. The results showed that the sloshing performance of the propellant tank was significantly affected by the internal damping structure. The research results of sloshing provide reference and support for the design and optimization of satellite attitude control.
Subclinical microvascular changes in ANCA-vasculitides: the role of optical coherence tomography angiography and nailfold capillaroscopy in the detection of disease-related damage.
Authors: Triggianese Paola , D'Antonio Arianna , Nesi Carolina , Kroegler Barbara , Marino Matteo Di , Conigliaro Paola , Modica Stella , Greco Elisabetta , Nucci Carlo , Bergamini Alberto , Chimenti Maria Sole , Cesareo Massimo 
Published: February 2023

Abstract
Background
Both cardiovascular and complement-mediated disorders might lead to microvascular damages in anti-neutrophil cytoplasm autoantibodies (ANCA)-associated vasculitides (AAV). We aimed at investigating, for the first time, subclinical microvascular abnormalities with non-invasive techniques in AAV patients by analyzing both retinal and nailfold capillary changes. Retinal plexi were investigated using optical coherence tomography angiography (OCT-A), while nailfold capillary changes by video-capillaroscopy (NVC). Potential correlations between microvessels’ abnormalities and disease damage were also explored.
Methods
An observational study was conducted on consecutive patients who met the inclusion criteria of defined diagnosis of eosinophilic granulomatosis with polyangiitis (EGPA), granulomatosis with polyangiitis (GPA), and microscopic polyangiitis (MPA), age ≥ 18 ≤ 75 yrs, and no ophthalmological disorders. Disease activity was assessed by Birmingham Vasculitis Activity Score (BVAS), damage by Vasculitis Damage Index (VDI), and poorer prognosis by the Five Factor Score (FFS). Quantitative analysis of vessel density (VD) was performed by OCT-A in both superficial and deep capillary plexi. Figures and detailed analysis from NVC were performed for all subjects in the study.
Results
Included AAV patients (n = 23) were compared with 20 age/sex-matched healthy controls (HC). Retinal VD in superficial whole and parafoveal plexi resulted significantly decreased in AAV compared to HC (P = 0.02 and P = 0.01, respectively). Furthermore, deep whole and parafoveal vessel density was strongly reduced in AAV than HC (P ≤ 0.0001 for both). In AAV patients, significant inverse correlations occurred between VDI and OCTA-VD in both superficial (parafoveal, P = 0.03) and deep plexi (whole, P = 0.003, and parafoveal P = 0.02). Non-specific NVC pattern abnormalities occurred in 82% of AAV patients with a similar prevalence (75%) in HC. In AAV, common abnormalities were edema and tortuosity in a comparable distribution with HC. Correlations between NVC changes and OCT-A abnormalities have not been described.
Conclusion
Subclinical microvascular retinal changes occur in patients with AAV and correlate with the disease-related damage. In this context, the OCT-A can represent a useful tool in the early detection of vascular damage. AAV patients present microvascular abnormalities at NVC, whose clinical relevance requires further studies.
Antioxidant effects of silver nanoparticles obtained by green synthesis from the aqueous extract of Eryngium carlinae on the brain mitochondria of streptozotocin-induced diabetic rats
Authors: Cruz-Hurtado Jenaro Lemus-de la , Trejo-Hurtado Mitchell , Landa-Moreno Cinthia , Peña-Montes Donovan , Landeros-Páramo José Luis , Cortés-Rojo Christian , Montoya-Pérez Rocío , Rosas Gerardo , Saavedra-Molina Alfredo 
Published: February 2023

Abstract
Diabetes mellitus is a metabolic disorder characterized by chronic hyperglycemia that affects practically all tissues and organs, being the brain one of most susceptible, due to overproduction of reactive oxygen species induced by diabetes. Eryngium carlinae is a plant used in traditional Mexican medicine to treat diabetes, which has already been experimentally shown have hypoglycemic, antioxidant and hypolipidemic properties. The green synthesis of nanoparticles is a technique that combines plant extracts with metallic nanoparticles, so that the nanoparticles reduce the absorption and distribution time of drugs or compounds, increasing their effectiveness. In this work, the antioxidant effects and mitochondrial function in the brain were evaluated, as well as the hypoglycemic and hypolipidemic effect in serum of both the aqueous extract of the aerial part of E. carlinae, as well as its combination with silver nanoparticles of green synthesis. Administration with both, extract and the combination significantly decreased the production of reactive oxygen species, lipid peroxidation, and restored the activity of superoxide dismutase 2, glutathione peroxidase, and electron transport chain complexes in brain, while that the extract-nanoparticle combination decreased blood glucose and triglyceride levels. The results obtained suggest that both treatments have oxidative activity and restore mitochondrial function in the brain of diabetic rats.
Design and application of laser scanning strategy for machining deep surface grooves with a continuous-wave fiber laser
Authors: Liu Si Qing , Han Sang Wook , Hwang Tae Woo , Abolhasani Daniyal , Moon Young Hoon 
Published: February 2023

Abstract
A laser scanning strategy for fabricating deep surface grooves using a continuous-wave fiber laser was investigated in this study. Because the low productivity of short-pulsed-wave lasers limits their application to a small scale, a continuous-wave (CW) fiber laser that can provide a high power density was used for rapid fabrication of deep grooves. An innovative tailored laser-scanning strategy of fabricating patterned deep grooves was analytically designed based on the power density and interaction time. Considering the thermophysical properties of the material, controlled laser processing parameters were determined for fabricating surface grooves with rectangular and chevron cross-sectional patterns. To confirm the usefulness of the research results, the scanning strategy obtained in this study was applied for achieving high-quality joining between injection-molded metal-plastic hybrids (MPHs). A deep-surface-grooved A5052 aluminum alloy sheet was bonded to two plastics, polyamide and polypropylene, via injection molding. Lap shear tensile tests of the MPHs revealed their significantly enhanced joining strength owing to a better mechanical interlocking of the groove. The developed laser scanning strategy using a CW fiber laser can be widely applied in the fabrication of deep grooves of various cross-sections with high reliability.
Research on automatic classification of Chinese papers based on LDA model and TF-IDF algorithm
Authors: Qizheng Li , Weilin Hu , Hao Dai 
Published: February 2023

Abstract
To ensure the automatic classification of research directions in designated fields along with the natural semantic machine recognition of research papers, this research proposes an automatic classification method based on topic probability. Initially, the feature words and their topic probabilities are calculated through the LDA topic model, and the weight of feature words in each paper is calculated by means of the TF-IDF algorithm. Finally, the topic probabilities of each feature word in the paper are weighted and normalized, thus accomplishing the topic probability distribution from words to papers. Through unsupervised classification experiments on 53,780 papers in the field of clothing, the accuracy rate reaches 92.4%, and the F-score reaches 85.0% in comparison with professional manual classification. Consequently, the probability classification of paper topics proposed in this research can be directly used to solve the automatic classification of papers and the automatic classification of research directions.
Effects of gabapentin on slow-wave sleep period in critically ill adult patients: a randomized controlled trial
Authors: Susantitapong Kanyarat , Sutherasan Yuda , Liamsombut Somprasong , Dilokpattanamongkol Pitchaya , Suthisisang Chuthamanee 
Published: February 2023

Abstract
Background: Sleep deprivation is a prevalent problem in critically ill patients, which leads to delayed recovery and delirium. Delirium prolongs the intensive care unit (ICU) and hospital lengths of stay and may increase mortality. Slow-wave sleep (SWS) is essential to energy restoration, tissue repair, and immune system strengthening. This study aimed to investigate the effects of gabapentin on SWS in critically ill patients.
Methods: We performed a prospective open-label randomized controlled study to compare SWS and the clinical outcomes of gabapentin (initial dose 100 mg at bedtime) versus a control intervention in critically ill adult patients admitted to the ICU within 24 hours. The patients’ characteristics and sleep-related outcomes were recorded. The sleep-related outcomes, namely bispectral analysis (BIS), the Richards-Campbell Sleep Questionnaire (RCSQ), and insulin-like growth factor-1 (IGF-1) levels, were evaluated. Furthermore, clinical outcomes, 28-day ICU-free days, 28-day mechanical ventilator (MV)-free days, 28-day hospital-free days, and safety were assessed.
Results: Sixty patients from 348 cases were eligible for randomization. On day 3 of the study, patients in the gabapentin group had significantly increased SWS (66.79 vs. 0.00 min; P < 0.001), total sleep time (TST) (331.39 vs. 46.16 min; P = 0.001), RCSQ score (55.05 ± 20.18 vs. 32.80 ± 15.31; P < 0.001), and insulin-like growth factor-1 (IGF-1) concentrations (84.33 ± 12.40 vs. 44. 00 ± 10.20 ng/mL, P < 0.001) compared with the control group. There was a trend in the improvement of clinical outcomes, such as delirium, ICU-free days, MV-free days, and hospital-free days; however, the differences were not significant. Over-sedation was reported in one case in the gabapentin group.
Conclusions: Gabapentin (100–200 mg) at bedtime increased SWS, TST, and IGF-1 concentrations in critically ill patients. This regimen might be beneficial to critically ill patients for improving their sleep quality.
Trial registration: The study was registered with ClinicalTrials.gov (NCT04818450), registered on March 25, 2021, https://clinicaltrials.gov/ct2/show/NCT04818450
How does road accessibility increase air pollution in the Yangtze River Delta Region, China ?-Evidence from digital elevation model and points-of-interest data
Authors: Tao Jing , Gu Xiaoyan 
Published: February 2023

Abstract
Empirical evidences on how urban road accessibility affects air pollution is scarce for the specific Yangtze River Delta (YRD) region that is characterised by high density in economic activities and rapid urban expansion. Using novel Digital Elevation Model (DEM), road network and points-of-interest (POI) datasets to measure road accessibility, moderating and mediating effect models then were conducted to explore direct and indirect effects of road accessibility on environmental externality expressed by air pollution through urban connection and urban agglomeration. When exploiting exogenous variable in urban populations, the result of moderating effect model shows that road accessibility decreases air quality-to-connection elasticity by around -5.2% which indicates that road accessibility increases air pollution through internal connection. However, the finding of mediating effect model indicates that road accessibility slightly improves air quality through decreasing urban agglomeration. The findings of this paper suggest that high levels of road accessibility may have negative consequence for live-ability of YRD cities because of current urban internal connection and urban decision makers should consider the improvement of urban connection for obtaining better air quality.