bluuebunny/crossref_metadata_2025_split huggingface dataset:
Suppressing Degradation in QLEDs via Doping ZnO Electron Transport Layer by Halides
Authors: Chung Dong Seob , Lyu Quan , Cotella Giovanni F. , Chun Peter , Aziz Hany 
Published: June 2023

AbstractRecent findings show that electron transport layers (ETLs) significantly influence the electroluminescence (EL) lifetime of quantum‐dot light‐emitting diodes (QLEDs). In this work, it is found that using halide dopants in the ZnO ETL significantly enhances device EL lifetime. Electrical, photoluminescence, and photoelectron spectroscopy measurements on QLEDs and specially designed devices are used for elucidating the root causes of the lifetime enhancement. Results show that charge transfer occurs at the ZnO/QDs interface in QLEDs, producing positively charged states in ZnO, subsequently leading to luminance loss, a mechanism that contributes to EL loss. Furthermore, XPS studies show that electrical stress of QLEDs leads to an increase in the concentration of ZnO species with higher oxidative states and that a correlation between the magnitude of EL loss and the concentration of these species exists. The use of halide dopants is found to reduce this interfacial charge transfer and the formation of the ZnO species with higher oxidative states, possibly due to the dopants role in acting as hole scavengers that trap and efficiently neutralize holes in ZnO. The findings underscore the significant role that managing positive charges in ZnO plays in EL lifetime and provide an effective strategy for achieving highly stable QLED.
Cathepsin K‐Activated Probe for Fluoro‐Photoacoustic Imaging of Early Osteolytic Metastasis
Authors: Song Zhuorun , Miao Jia , Miao Minqian , Cheng Baoliang , Li Shenhua , Liu Yinghua , Miao Qingqing , Li Qing , Gao Mingyuan 
Published: June 2023

AbstractPrecise detection of early osteolytic metastases is crucial for their treatment but remains challenging in the clinic because of the limited sensitivity and specificity of traditional imaging techniques. Although fluorescence imaging offers attractive features for the diagnosis of osteolytic metastases, it is hampered by limited penetration depth. To address this issue, a fluoro‐photoacoustic dual‐modality imaging probe comprising a near‐infrared dye caged by a cathepsin K (CTSK)‐cleavable peptide sequence on one side and functionalized with osteophilic alendronate through a polyethylene glycol linker on the other side is reported. Through systematic in vitro and in vivo experiments, it is demonstrated that in response to CTSK, the probe generated both near‐infrared fluorescent and photoacoustic signals from bone metastatic regions, thus offering a potential strategy for detecting deep‐seated early osteolytic metastases.
A New Type of Endometrial Cancer Models in Mice Revealing the Functional Roles of Genetic Drivers and Exploring their Susceptibilities
Authors: Chen Jingyao , Dai Siqi , Zhao Lei , Peng Yiman , Sun Chongen , Peng Hongling , Zhong Qian , Quan Yuan , Li Yue , Chen Xuelan , Pan Xiangyu , Zhong Ailing , Wang Manli , Zhang Mengsha , Yang Shengyong , Lu You , Lian Zhong , Liu Yu , Zhou Shengtao , Li Zhengyu , Na Feifei , Chen Chong 
Published: June 2023

AbstractEndometrial cancer (EC) is the most common female reproductive tract cancer and its incidence has been continuously increasing in recent years. The underlying mechanisms of EC tumorigenesis remain unclear, and efficient target therapies are lacking, for both of which feasible endometrial cancer animal models are essential but currently limited. Here, an organoid and genome editing‐based strategy to generate primary, orthotopic, and driver‐defined ECs in mice is reported. These models faithfully recapitulate the molecular and pathohistological characteristics of human diseases. The authors names these models and similar models for other cancers as organoid‐initiated precision cancer models (OPCMs). Importantly, this approach can conveniently introduce any driver mutation or a combination of driver mutations. Using these models,it is shown that the mutations in Pik3ca and Pik3r1 cooperate with Pten loss to promote endometrial adenocarcinoma in mice. In contrast, the Kras G12D mutati led to endometrial squamous cell carcinoma. Then, tumor organoids are derived from these mouse EC models and performed high‐throughput drug screening and validation. The results reveal distinct vulnerabilities of ECs with different mutations. Taken together, this study develops a multiplexing approach to model EC in mice and demonstrates its value for understanding the pathology of and exploring the potential treatments for this malignancy.
Understanding the Bifunctional Trends of Fe‐Based Binary Single‐Atom Catalysts
Authors: Li Ruisong , Rao Peng , Wu Daoxiong , Li Jing , Deng Peilin , Miao Zhengpei , Tian Xinlong 
Published: June 2023

AbstractBinary single‐atom catalysts (BSACs) have demonstrated fascinating activities compared to single atom catalysts (SACs) for oxygen reduction reaction (ORR) and oxygen evolution reaction (OER). Notably, Fe SACs is one of the most promising ORR electrocatalysts, and further revealing the synergistic effects between Fe and other 3d transition metals (M) for FeM BSACs are very important to enhance bifunctional performance. Herein, density functional theory (DFT) calculations are first adapted to demonstrate the role of various transition metals on the bifunctional activity of Fe sites, and a notable volcano relationship is established through the generally accepted adsorption free energy that ΔG*OH for ORR, and ΔG*O−ΔG*OH for OER, respectively. Further, ten of the atomically dispersed FeM anchored on nitrogen‐carbon support (FeM‐NC) are successfully synthesized with typical atomic dispersion by a facile movable type printing method. The experimental data confirms the bifunctional activity diversity of FeM‐NC between the early‐ and late‐ transition metals, agrees very well with the DFT results. More importantly, the optimal FeCu‐NC shows the expected performance with high ORR and OER activity, thereby, the assembled rechargeable zinc–air battery delivers a high power density of 231 mW cm−2, and an impressive stability that can be stably operated over 300 h.
CRISPR/Cas13a‐Based MicroRNA Detection in Tumor‐Derived Extracellular Vesicles
Authors: Hong Jae‐Sang , Son Taehwang , Castro Cesar M. , Im Hyungsoon 
Published: June 2023

AbstractMicroRNAs (miRNAs) in extracellular vesicles (EVs) play essential roles in cancer initiation and progression. Quantitative measurements of EV miRNAs are critical for cancer diagnosis and longitudinal monitoring. Traditional PCR‐based methods, however, require multi‐step procedures and remain as bulk analysis. Here, the authors introduce an amplification‐free and extraction‐free EV miRNA detection method using a CRISPR/Cas13a sensing system. CRISPR/Cas13a sensing components are encapsulated in liposomes and delivered them into EVs through liposome‐EV fusion. This allows for accurately quantify specific miRNA‐positive EV counts using 1 × 108 EVs. The authors show that miR‐21‐5p‐positive EV counts are in the range of 2%–10% in ovarian cancer EVs, which is significantly higher than the positive EV counts from the benign cells (<0.65%). The result show an excellent correlation between bulk analysis with the gold‐standard method, RT‐qPCR. The authors also demonstrate multiplexed protein‐miRNA analysis in tumor‐derived EVs by capturing EpCAM‐positive EVs and quantifying miR‐21‐5p‐positive ones in the subpopulation, which show significantly higher counts in the plasma of cancer patients than healthy controls. The developed EV miRNA sensing system provides the specific miRNA detection method in intact EVs without RNA extraction and opens up the possibility of multiplexed single EV analysis for protein and RNA markers.
A NIR‐II Photoactivatable “ROS Bomb” with High‐Density Cu2O‐Supported MoS2 Nanoflowers for Anticancer Therapy
Authors: Huang Jia , Deng Guiming , Wang Shuya , Zhao Tianjiao , Chen Qiaohui , Yang Yuqi , Yang Yongqi , Zhang Jinping , Nan Yayun , Liu Zhaoqian , Cao Ke , Huang Qiong , Ai Kelong 
Published: June 2023

AbstractThe fast conversion of hydrogen peroxide (H2O2) into reactive oxygen species (ROS) at tumor sites is a promising anticancer strategy by manipulating nanomedicines with near‐infrared light in the second region (NIR‐II). However, this strategy is greatly compromised by the powerful antioxidant capacity of tumors and the limited ROS generation rate of nanomedicines. This dilemma mainly stems from the lack of an effective synthesis method to support high‐density copper‐based nanocatalysts on the surface of photothermal nanomaterials. Herein, a multifunctional nanoplatform (MCPQZ) with high–density cuprous (Cu2O) supported molybdenum disulfide (MoS2) nanoflowers (MC NFs) is developed for the efficient killing of tumors via a potent ROS storm by an innovative method. Under NIR‐II light irradiation, the ROS intensity and maximum reaction velocity (Vmax) produced by MC NFs are 21.6 and 33.8 times that of the non–irradiation group in vitro, which is much higher than most current nanomedicines. Moreover, the strong ROS storm in cancer cells is efficiently formed by MCPQZ (increased by 27.8 times compared to the control), thanks to the fact that MCPQZ effectively pre–weakens the multiple antioxidant systems of cancer cells. This work provides a novel insight to solve the bottleneck of ROS‐based cancer therapy.
Electroceuticals for Regeneration of Long Nerve Gap Using Biodegradable Conductive Conduits and Implantable Wireless Stimulator
Authors: Kim Jio , Jeon Jooik , Lee Ju‐Yong , Khoroldulam Badamgarav , Choi Sung‐Geun , Bae Jae‐Young , Hyun Jung Keun , Kang Seung‐Kyun 
Published: June 2023

AbstractRegeneration of over 10 mm long peripheral nerve defects remains a challenge due to the failure of regeneration by prolonged axotomy and denervation occurring in long‐term recovery. Recent studies reveal that conductive conduits and electrical stimulation accelerate the regeneration of long nerve defects. In this study, an electroceutical platform combining a fully biodegradable conductive nerve conduit and a wireless electrical stimulator is proposed to maximize the therapeutic effect on nerve regeneration. Fully biodegradable nerve conduit fabricated using molybdenum (Mo) microparticles and polycaprolactone (PCL) can eliminate the unwanted effects of non‐degradable implants, which occupy nerve paths and need to be removed through surgery increasing the risk of complications. The electrical and mechanical properties of Mo/PCL conduits are optimized by controlling the amounts of Mo and tetraglycol lubricant. The dissolution behavior and electrical conductivity of biodegradable nerve conduits in the biomimetic solutions are also evaluated. In in vivo experiments, the integrated strategy of a conductive Mo/PCL conduit with controlled therapeutic electrical stimulation shows accelerated axon regeneration for long sciatic nerve defects in rats compared to the use of the Mo/PCL conduit without stimulation and has a significant therapeutic effect based on the results obtained from the functional recovery test.
Adaptive function of duodichogamy: Why do chestnut trees have two pollen emission phases?
Authors: Pauly Grégoire , Larue Clément , Petit Rémy J. 
Published: June 2023

AbstractPremiseIntersexual mating facilitation in flowering plants has been largely underexplored. Duodichogamy is a rare flowering system in which individual plants flower in the sequence male‐female‐male. We studied the adaptive advantages of this flowering system using chestnuts (Castanea spp., Fagaceae) as models. These insect‐pollinated trees produce many unisexual male catkins responsible for a first staminate phase and a few bisexual catkins responsible for a second staminate phase. We hypothesized that duodichogamy increases female mating success by facilitating pollen deposition on stigmas of the rewardless female flowers through their proximity with attractive male flowers responsible for the minor staminate phase.MethodsWe monitored insect visits to 11 chestnut trees during the entire flowering period and explored reproductive traits of all known duodichogamous species using published evidence.ResultsIn chestnuts, insects visited trees more frequently during the first staminate phase but visited female flowers more frequently during the second staminate phase. All 21 animal‐pollinated duodichogamous species identified are mass‐flowering woody plants at high risk of self‐pollination. In 20 of 21 cases, gynoecia (female flower parts) are located close to androecia (male flower parts), typically those responsible for the second minor staminate phase, whereas androecia are often distant from gynoecia.ConclusionsOur results suggest that duodichogamy increases female mating success by facilitating pollen deposition on stigmas by means of the attractiveness of the associated male flowers while effectively limiting self‐pollination.
Chemical Fixation of Carbon Dioxide into Cyclic Carbonates Catalyzed by Porous Materials
Authors: Wang Qing , Chen Peibo , Li Xuejun , Liang Ying , Pan Yingming 
Published: June 2023

AbstractThe reaction of carbon dioxide (CO2) with epoxide to generate cyclic carbonates is one of the most effective chemical fixation methods. The catalytic system significantly affects the carbon fixation efficiency of the reaction. Among the reported catalytic systems, porous materials as heterogeneous catalysts have attracted significant attention. This study aimed to summarize porous materials that catalyzed the conversion of CO2 into cyclic carbonates, including metal organic frameworks, covalent organic frameworks, poly(ionic) liquids, hyper crosslinked polymers, conjugated porous polymers and others. The study analyzed the catalytic properties of these materials and outlined future research directions.
MEMOR: A database of archeological human remains collections from Flanders, Belgium
Authors: De Groote Isabelle , Van de Vijver Katrien , Veselka Barbara , De Potter Pieterjan , Massagé Liesbeth , Van der Dooren Lien , Vandenborre Jeroen , Larmuseau Maarten H. D. , Danckers Jonas , Robberechts Bart 
Published: June 2023

AbstractThe aim of this article is to describe a newly created open access database of archeological human remains collections from Flanders, Belgium. The MEMOR database (www.memor.be) was created to provide an overview of the current practices of loans, reburial, and the research potential of human skeletons from archeological sites currently stored in Flanders. In addition, the project aimed to provide a legal and ethical framework for the handling of human remains and was created around stakeholder involvement from anthropologists, geneticists, contract archeologists, the local, regional and national government agencies, local and national government, universities, and representatives of the major religions. The project has resulted in the creation of a rich database with many collections available for study. The database was created using the open‐source Arches data management platform that is freely available for organizations worldwide to configure in accordance with their individual needs and without restrictions on its use. Each collection is linked to information about the excavation and the site the remains originate from, its size and time period. In addition, a research potential tab reveals whether any analyses were performed, and whether excavation notes are available with the assemblage. The database currently contains 742 collections, ranging in size from 1 to over 1000 individuals. New collections will continue to be added when new assemblages are excavated and studied. The database can also be expanded to include human remains collections from other regions and other material categories, such as archaeozoological collections.
GluK2 Is a Target for Gene Therapy in Drug‐Resistant Temporal Lobe Epilepsy
Authors: Boileau Céline , Deforges Severine , Peret Angélique , Scavarda Didier , Bartolomei Fabrice , Giles April , Partouche Nicolas , Gautron Justine , Viotti Julio , Janowitz Haley , Penchet Guillaume , Marchal Cécile , Lagarde Stanislas , Trebuchon Agnès , Villeneuve Nathalie , Rumi Julie , Marissal Thomas , Khazipov Roustem , Khalilov Ilgam , Martineau Fanny , Maréchal Marine , Lepine Anne , Milh Mathieu , Figarella‐Branger Dominique , Dougy Etienne , Tong Soutsakhone , Appay Romain , Baudouin Stéphane , Mercer Andrew , Smith Jared B. , Danos Olivier , Porter Richard , Mulle Christophe , Crépel Valérie 
Published: June 2023

ObjectiveTemporal lobe epilepsy (TLE) is characterized by recurrent seizures generated in the limbic system, particularly in the hippocampus. In TLE, recurrent mossy fiber sprouting from dentate gyrus granule cells (DGCs) crea an aberrant epileptogenic network between DGCs which operates via ectopically expressed GluK2/GluK5‐containing kainate receptors (KARs). TLE patients are often resistant to anti‐seizure medications and suffer significant comorbidities; hence, there is an urgent need for novel therapies. Previously, we have shown that GluK2 knockout mice are protected from seizures. This study aims at providing evidence that downregulating KARs in the hippocampus using gene therapy reduces chronic epileptic discharges in TLE.MethodsWe combined molecular biology and electrophysiology in rodent models of TLE and in hippocampal slices surgically resected from patients with drug‐resistant TLE.ResultsHere, we confirmed the translational potential of KAR suppression using a non‐selective KAR antagonist that markedly attenuated interictal‐like epileptiform discharges (IEDs) in TLE patient‐derived hippocampal slices. An adeno‐associated virus (AAV) serotype‐9 vector expressing anti‐grik2 miRNA was engineered to specifically downregulate GluK2 expression. Direct delivery of AAV9‐anti grik2 miRNA into the hippocampus of TLE mice led to a marked reduction in seizure activity. Transduction of TLE patient hippocampal slices reduced levels of GluK2 protein and, most importantly, significantly reduced IEDs.InterpretationOur gene silencing strategy to knock down aberrant GluK2 expression demonstrates inhibition of chronic seizure in a mouse TLE model and IEDs in cultured slices derived from TLE patients. These results provide proof‐of‐concept for a gene therapy approach targeting GluK2 KARs for drug‐resistant TLE patients. ANN NEUROL 2023;94:745–761
An Efficient Direct Arylation Polycondensation via C−S Bond Cleavage
Authors: Zhang Meng , Zhang Bei‐Bei , Lin Qijie , Jiang Ziling , Zhang Jianqi , Li Yawen , Pei Shurui , Han Xiao , Xiong Haigen , Liang Xinyu , Lin Yuze , Wei Zhixiang , Zhang Fengjiao , Zhang Xin , Wang Zhi‐Xiang , Shi Qinqin , Huang Hui 
Published: June 2023

AbstractThe direct arylation polycondensation (DArP) has become one of the most important methods to construct conjugated polymers (CPs). However, the homocoupling side‐reactions of aryl halides and the low regioseletive reactivities of unfunctionalized aryls hinder the development of DArP. Here, an efficient Pd and Cu co‐catalyzed DArP was developed via inert C−S bond cleavage of aryl thioethers, of which robustness was exemplified by over twenty conjugated polymers (CPs), including copolymers, homopolymers, and random polymers. The capture of oxidative addition intermediate together with experimental and theoretic results suggested the important role of palladium (Pd) and copper (Cu) co‐catalysis with a bicyclic mechanism. The studies of NMR, molecular weights, trap densities, two‐dimensional grazing‐incidence wide‐angle X‐ray scattering (2D‐GIWAXS), and the charge transport mobilities revealed that the homocoupling reactions were significantly suppressed with high regioselectivity of unfunctionalized aryls, suggesting this method is an excellent choice for synthesizing high performance CPs.
Towards a Precise NMR Quantification of Acute Phase Inflammation Proteins from Human Serum
Authors: Mallagaray Alvaro , Rudolph Lorena , Lindloge Melissa , Mölbitz Jarne , Thomsen Henrik , Schmelter Franziska , Alhabash Mohamad Ward , Abdullah Mohammed R. , Saraei Roza , Ehlers Marc , Graf Tobias , Sina Christian , Petersmann Astrid , Nauck Matthias , Günther Ulrich L. 
Published: June 2023

AbstractNuclear Magnetic Resonance (NMR) spectra of human serum and plasma show, besides metabolites and lipoproteins, two characteristic signals termed GlycA and B arising from the acetyl groups of glycoprotein glycans from acute phase proteins, which constitute good markers for inflammatory processes. Here, we report a comprehensive assignment of glycoprotein glycan NMR signals observed in human serum, showing that GlycA and GlycB signals originate from Neu5Ac and GlcNAc moieties from N‐glycans, respectively. Diffusion‐edited NMR experiments demonstrate that signal components can be associated with specific acute phase proteins. Conventionally determined concentrations of acute phase glycoproteins correlate well with distinct features in NMR spectra (R2 up to 0.9422, p‐value <0.001), allowing the simultaneous quantification of several acute phase inflammation proteins. Overall, a proteo‐metabolomics NMR signature of significant diagnostic potential is obtained within 10–20 min acquisition time. This is exemplified in serum samples from COVID‐19 and cardiogenic shock patients showing significant changes in several acute phase proteins compared to healthy controls.
Synthesis, Structural Characterization and Coordination Chemistry of a NHC‐stabilized Iminoborane
Authors: Yue Xin , Wu Linlin , Wang Hao 
Published: June 2023

AbstractNHC‐stabilized iminoborane4was prepared and structurally characterized via the reduction of a cationic iminoborane with potassium graphite. Compound4could be served as a supporting ligand for the synthesis of both main group and transition metal complexes through variable coordination modes. This study demonstrates the rich coordination chemistry of the Lewis base‐stabilized iminoborane.
Orthogonal Glycosylation with Phosphate Acceptors for Expeditious Synthesis of Bacterial Inner Core Oligosaccharides
Authors: Lu I‐Chen , Cheng Kuang‐Chun , Wang Yi‐Fang , Pan Chia‐Wei , Hung Jan‐Siang , Mong Kwok‐Kong Tony 
Published: June 2023

AbstractWe report a practical one‐pot glycosylation strategy for synthesis of bacterial inner core oligosaccharides that composed of unavailable L‐glycero‐D‐manno and D‐glycero‐D‐manno‐heptopyranose components. The glycosylation method features a new orthogonal glycosylation procedure; whereby a phosphate acceptor is coupled with a thioglycosyl donor producing a disaccharide phosphate, which can be engaged in another orthogonal glycosylation procedure to couple with a thioglycosyl acceptor. The phosphate acceptors used in above one‐pot procedure are directly prepared from thioglycosyl acceptors via the in‐situ phosphorylation. Such phosphate acceptor preparation protocol eliminates the traditional protection and deprotection procedures. Based on the new one‐pot glycosylation strategy, two partial inner core structures of Yersinia pestis lipopolysaccharide and Haemophilus ducreyi lipooligosaccharide were acquired.
Development of On‐DNA Thiophene Synthesis for DEL Construction
Authors: Luo Ayun , Duchemin Nicolas , Wang Xiuming , Zhou Hongxia , Zeng Fanming , Zhao Xue , Yu Weina , Yang Kexin , Jin Hu Yun 
Published: June 2023

AbstractThiophene and its substituted derivatives are a highly important class of heterocyclic compounds, with noteworthy applications in pharmaceutical ingredients. In this study, we leverage the unique reactivity of alkynes to generate thiophenes on‐DNA, using a cascade iodination, Cadiot‐Chodkiewicz coupling and heterocyclization. This approach, tackling on‐DNA thiophene synthesis for the first time, generates diverse, and unprecedented structural and chemical features, which could be significant motifs in DEL screening as molecular recognition agents for drug discovery.