bluuebunny/crossref_metadata_2025_split huggingface dataset:
Hypoxia exacerbates heat stress effects on the porcine intestinal epithelium in vitro
Authors: Pearce S. C. , Gabler N. K. 
Published: July 2023

Heat stress (HS) negatively impacts human health, as well as animal agriculture. The mechanisms underlying HS-induced intestinal dysfunction in vivo are still not fully elucidated. However, HS has been shown to cause intestinal ischemia/hypoxia, which contributes to reduced barrier integrity. The objective of this study was to examine hypoxia alone, HS alone, and a combination using IPEC-J2 cells. We hypothesized that hypoxia is a critical factor and important step in the pathway to HS-induced barrier dysfunction. Porcine IPEC-J2 cells were grown in Transwell™ plates and then treated either under thermal neutral (TN; 38°C) or heat stress (HS; 42°C) and either normoxia (NX; ~21% O2) or hypoxia (HX; 1% O2) conditions for 24 h. Transepithelial electrical resistance, paracellular permeability marker, FITC-dextran, media interleukin 8, cell HSP70 and 90, CLDN4, ZO-1, and EEA1 were all analyzed. Results showed that HS did not increase intestinal permeability in this model and elicited a reduction in IL-8 while still exhibiting a robust HSP response. In this model, hypoxia was required to induce intestinal barrier dysfunction and TJ redistribution. The combination of HS and hypoxia caused even more severe tight junction disruption. This was accompanied by the absence of an IL-8 response under HS.
Knowledge, attitude, and proficiency of healthcare providers in cardiopulmonary resuscitation in a public primary healthcare setting in Qatar
Authors: Veettil Shajitha Thekke , Anodiyil Mohamed Shaheen , Khudadad Hanan , Kalathingal Mohamed Ali , Hamza Abdul Hakeem , Ummer Femina Purakaloth , Alnuaimi Ahmed Sameer 
Published: July 2023

IntroductionEarly and effective cardiopulmonary resuscitation (CPR) increases both survival rate and post–cardiac arrest quality of life. This study aims to assess the current knowledge and ability of physicians and nurses in health centers (HCs) operated by the Primary Health Care Corporation (PHCC) in Doha, Qatar, to perform CPR.MethodologyThis study consists of two parts. The first part is a descriptive cross-sectional survey using an online form targeting physicians and nurses working in all HCs to assess their CPR knowledge and attitude. The second part is a direct observation of CPR drills to evaluate the skills and competencies of code blue team members in a convenient sample of 14 HCs. A multivariate model was employed to test the independent effect of explanatory variables on the total knowledge score.ResultsA total of 569 physicians and nurses responded to the survey. Only one-half (48.7%) formally received training on basic life support within the last year. Furthermore, 62.7% have tried to revive a dying person with no pulse. All the participants recognize the importance of knowing how to revive a dying adult or child as part of their job. The study showed that being a nurse was the most important predictor of a higher knowledge score in both components. Attending more resuscitation courses (3–6 courses in the last 3 years) ranked second in importance, and a longer experience in clinical practice (5–10 and &gt;10 years) ranked third in predictive power. In addition, the direct observation of CPR drill performance revealed a satisfactory outcome.ConclusionThe level of CPR knowledge and skills practice among healthcare providers in PHCC is deemed satisfactory as most providers reported having performed CPR in the past. Considering that PHCC is the first step to people’s healthcare in Qatar, clinical staff should be certified and assessed regularly to ensure retention of resuscitation knowledge and skills.
Current and future sinkhole susceptibility in karst and pseudokarst areas of the conterminous United States
Authors: Wood Nathan J. , Doctor Daniel H. , Alder Jay , Jones Jeanne 
Published: July 2023

Sinkholes in karst and pseudokarst regions threaten infrastructure, property, and lives. We mapped closed depressions in karst and pseudokarst regions of the conterminous United States (U.S.) from 10-m-resolution elevation data using high-performance computing, and then created a heuristic additive model of sinkhole susceptibility that also included nationally consistent data for factors related to geology, soils, precipitation extremes, and development. Maps identify potential sinkhole hotspots based on current conditions and projections for 50 years into the future (the years 2070–2079) based on climate change and urban development scenarios. Areas characterized as having either high or very high sinkhole susceptibility contain 94%–99% of known or probable sinkhole locations from three U.S. state databases. States and counties with the highest amounts and percentages of land in zones of highest sinkhole susceptibility are identified. Projected changes in extreme precipitation and development did not substantially change current hotspots of highest sinkhole susceptibility. Results provide a uniform index of sinkhole potential that can support national planning, instead of existing assessments produced through various methods within individual states or smaller areas.
Ear canal pressure sensor for food intake detection
Authors: Hossain Delwar , Ghosh Tonmoy , Haider Imtiaz Masudul , Sazonov Edward 
Published: July 2023

Introduction: This paper presents a novel Ear Canal Pressure Sensor (ECPS) for objective detection of food intake, chew counting, and food image capture in both controlled and free-living conditions. The contribution of this study is threefold: 1) Development and validation of a novel wearable sensor that uses changes in ear canal pressure and the device’s acceleration as an indicator of food intake, 2) A method to identify chewing segments and count the number of chews in each eating episode, and 3) Facilitation of egocentric image capture only during eating by triggering camera from sensor detection thus reducing power consumption, privacy concerns, as well as storage and computational cost.Methods: To validate the device, data were collected from 10 volunteers in a controlled environment and three volunteers in a free-living environment. During the controlled activities, each participant wore the device for approximately 1 h, and during the free living for approximately 12 h. The food intake of the participants was not restricted in any way in both part of the experiment. Subject-independent Support Vector Machine classifiers were trained to identify periods of food intake from the features of both the pressure sensor and accelerometer, and features only from the pressure sensor.Results: Results from leave-one-out cross-validation showed an average 5 sec-epoch classification F-score of 87.6% using only pressure sensor features and 88.6% using features from both pressure sensor and accelerometer in the controlled environment. For the free-living environment, both classifiers accurately detected all eating episodes. The wearable sensor achieves 95.5% accuracy in counting the number of chews with respect to manual annotation from the videos of the eating episodes using a pressure sensor classifier in the controlled environment.Discussion: The manual review of the images found that only 3.7% of captured images belonged to the detected eating episodes, suggesting that sensor-triggered camera capture may facilitate reducing the number of captured images and power consumption of the sensor.
Prevalence of chronic kidney disease and associated factors among patients with underlying chronic disease at Dessie Referral Hospital, East Amhara Region, Ethiopia
Authors: Ali Ahmed , Mulatu Kebadnew , Feleke Sefineh Fenta , Wassie Gizachew Tadesse 
Published: July 2023

BackgroundChronic kidney disease is defined as a reduction in glomerular filtration rate below 60 ml/min per 1.73 m2 and presence of albuminuria over a period of time. Globally, 10%–15% of populations are affected by chronic kidney disease. Studies conducted in Jimma, Addis Ababa, and the Tigray region were conducted on a single chronic disease and did not include human immune viruses. In addition, there has been no such study conducted in the Amhara region. Therefore, the aim of this study was to determine the magnitude and associated factors of chronic kidney disease among chronic patients who are followed up at an outpatient department.MethodsAn institutional-based cross-sectional study of 480 chronic patients was conducted at Dessie Referral Hospital, Dessie, Ethiopia between 15 March and 16 April 2020. Data were entered into Epidata and exported to SPSS version 25 for analysis. Binary logistic regression models were performed to identify factors associated with chronic kidney disease. The variables with a p-value ≤0.25 were considered to be a candidate for multivariable logistic regression. A p-value ≤0.05 was considered a statistically significant association.ResultsThe magnitude of chronic kidney disease among the study participants was 21.3%. Variables such as hypertension [adjusted odds ratio (AOR): 2.6, 95% CI: 1.58–4.27], use of non-steroidal anti-inflammatory drugs (AOR: 2.4, 95% CI: 1.41–3.97), smoking (AOR: 4.4, 95% CI: 2.65–7.34), routine physical activity (AOR: 0.6, 95% CI: 0.35–0.94), and obesity (AOR: 3.0, 95% CI: 1.76–5.05) were significantly associated with the chronic kidney disease.ConclusionThis study found that the magnitude of chronic kidney disease in the study area was high. Hypertension, use of non-steroidal anti-inflammatory drugs, smoking, routine physical activity, and obesity were found to be significant factors for chronic kidney disease.
Burnout syndrome and work engagement in nursing staff: a systematic review and meta-analysis
Authors: Vargas-Benítez Miguel Ángel , Izquierdo-Espín Francisco José , Castro-Martínez Nuria , Gómez-Urquiza José L. , Albendín-García Luis , Velando-Soriano Almudena , Cañadas-De la Fuente Guillermo A. 
Published: July 2023

BackgroundA difficult and demanding work environment, such as that often experienced in healthcare, can provoke fatigue, anxiety, distress, and discomfort. This study considers factors that may influence levels of burnout and work engagement among nurses and seeks to determine the relationship between these conditions.MethodA systematic scoping review was performed, in accordance with the PRISMA Extension for Scoping Reviews, based on data obtained from a search of the PubMed/MEDLINE and Scopus databases carried out in 2022 using the search equation: “work engagement AND nurs* AND burnout.” This search identified nine quantitative primary studies suitable for inclusion in our analysis.ResultsWork overload, type of shift worked, and/or area of hospital service, among other elements, are all relevant to the development of burnout. This syndrome can be countered by social support and appropriate personal resources and values, which are all positively associated with work engagement. Our analysis revealed a significant correlation between work engagement and the different domains of burnout. The correlation effect size between burnout and work engagement was −0.46 (95% CI −0.58, −0.31), with p &lt; 0.001.ConclusionWell-targeted interventions in the healthcare work environment can reduce burnout levels, strengthen work engagement, and enhance the quality of healthcare.
Physiotherapists’ ethical behavior in professional practice: a qualitative study
Authors: Mármol-López María Isabel , Marques-Sule Elena , Naamanka Kati , Arnal-Gómez Anna , Cortés-Amador Sara , Durante Ángela , Tejada-Garrido Clara Isabel , Navas-Echazarreta Noelia , Juárez-Vela Raúl , Gea Vicente 
Published: July 2023

BackgroundIn health professions, ethics is considered a fundamental competence. The increase in clinical autonomy in the field of physiotherapy is associated with an increase in ethical situations in their clinical practice.ObjectiveTo explore the ethics of the clinical relationship between physiotherapists and patients, the ethics training received by physiotherapists, and if in the clinical context, physiotherapists identify the necessary attitudes and apply the ethical recommendations of the profession for the ethical situations they experience.MethodsA qualitative exploratory and descriptive study was performed with physiotherapists. Data were collected through semi-structured interviews. The data were analyzed using content analysis, as proposed by Krippendorf. The study protocol was approved by the University of Valencia Ethics Committee of Human Research.ResultsThis study included 15 physiotherapists (66.66% women, average age = 42.2 years), which was sufficient to reach data saturation. We identified four categories: (i) Ethics of the clinical relationship (ethical values, principles, and norms; type of clinical relationship), (ii) Ethics training received (during the physiotherapy studies; current training of students; low importance of ethics in the curriculum), (iii) Necessary attitudes for professional ethical practice (main attitudes were identified: personal attitudes and professional attitudes); (iv) Experiences from professional practice (general; public sector vs. private sector).ConclusionThe ethics of the clinical relationship between physiotherapists and patients is determined by the attitudes of the practitioner, which are the result of his or her values and previous experiences; and are very centered on ethics of indication (founded mainly on the principles of Beneficence and Non-Maleficence). It is necessary to improve the ethical training received by physiotherapists, which is poorly focused on professional attitudes.
The value of multimodal ultrasonography in evaluating therapeutic response of cervical tuberculous lymphadenitis to anti-tuberculosis drugs
Authors: Yu Tianzhuo , Zhang Lin , Xu Jianping , Meng Jun , Yu Xiulei , Zhang Ying 
Published: July 2023

PurposeIn order to research the value of multimodal ultrasonography in evaluating therapeutic response of cervical tuberculous lymphadenitis to anti-tuberculosis drugs.Materials and methodsSixty-one patients with cervical tuberculous lymphadenitis were enrolled in this study. Ultrasound examination was performed before systemic standard anti-tuberculosis treatment and within 1–2 months after treatment, and the patients were divided into effective group and ineffective group according to the follow-up at the sixth month. The multimodal ultrasound signs of the two groups were compared and analyzed.ResultsIn the effective group, there were significant differences in the maximum diameter of lymph nodes, the echo of the surrounding tissue and the enlargement of the contrast area before and after treatment (p &lt; 0.05). At 1–2 months after treatment, there were significant differences in the maximum diameter, pus changes, CDFI, elasticity scores, echo of surrounding tissues, changes in enlarged and non-enhanced areas after contrast enhancement between the effective group and the ineffective group (p &lt; 0.05).ConclusionThe multimodal ultrasound signs of the appearance of internal pus or non-enhancement area enlargement, enhanced echo of the surrounding tissue and enlargement after CEUS are related to poor prognosis, and may be used to evaluate the response of anti-tuberculosis chemotherapy when the size change of lymph node is not obvious in individual treatment.
Anopheline mosquito saliva contains bacteria that are transferred to a mammalian host through blood feeding
Authors: Accoti Anastasia , Damiani Claudia , Nunzi Emilia , Cappelli Alessia , Iacomelli Gloria , Monacchia Giulia , Turco Antonella , D’Alò Francesco , Peirce Matthew J. , Favia Guido , Spaccapelo Roberta 
Published: July 2023

IntroductionMalaria transmission occurs when Plasmodium sporozoites are transferred from the salivary glands of anopheline mosquitoes to a human host through the injection of saliva. The need for better understanding, as well as novel modes of inhibiting, this key event in transmission has driven intense study of the protein and miRNA content of saliva. Until now the possibility that mosquito saliva may also contain bacteria has remained an open question despite the well documented presence of a rich microbiome in salivary glands.MethodsUsing both 16S rRNA sequencing and MALDI-TOF approaches, we characterized the composition of the saliva microbiome of An. gambiae and An. stephensi mosquitoes which respectively represent two of the most important vectors for the major malaria-causing parasites P. falciparum and P. vivax.ResultsTo eliminate the possible detection of non-mosquito-derived bacteria, we used a transgenic, fluorescent strain of one of the identified bacteria, Serratiamarcescens, to infect mosquitoes and detect its presence in mosquito salivary glands as well as its transfer to, and colonization of, mammalian host tissues following a mosquito bite. We also showed that Plasmodium infection modified the mosquito microbiota, increasing the presence of Serratia while diminishing the presence of Elizabethkingia and that both P. berghei and Serratia were transferred to, and colonized mammalian tissues.DiscussionThese data thus document the presence of bacteria in mosquito saliva, their transfer to, and growth in a mammalian host as well as possible interactions with Plasmodium transmission. Together they raise the possible role of mosquitoes as vectors of bacterial infection and the utility of commensal mosquito bacteria for the development of transmission-blocking strategies within a mammalian host.
Flint corn silage management: influence of maturity stage, inoculation with Lentilactobacillus buchneri, and storage time on fermentation pattern, aerobic stability, and nutritional characteristics
Authors: Rossi Luis G. , Andrade Marina E. B. , Rabelo Carlos H. S. , Siqueira Gustavo R. , Vicente Eduardo F. , Silva Wilton L. , Silva Matheus M. , Reis Ricardo A. 
Published: July 2023

IntroductionHigh quality corn silage depends on factors such as corn type, stage of crop development at harvest time, fermentation time, in addition to use or not of inoculants. This study aimed to investigate the impact of maturity stage, bacterial inoculation, and storage time on fermentation, aerobic stability, and nutritional characteristics of flint corn silage and their implications for corn silage management.MethodsA flint corn hybrid was harvested very early, early, and medium (at 250, 300 and 350 g dry matter (DM)/kg as fed, respectively) and ensiled in mini-silos without (control) or with Lentilactobacillus buchneri CNCM I-4323 at 1 × 105 cfu/g for 120, 240 and 360 d to investigate how these factors interact with each other.Results and discussionThere was only a small increase (7 g/kg starch; p = 0.003) in starch digestibility (starch-D) in the silages stored for 360 d when compared to that stored for 240 d, but with no difference for 120 d. Despite the reduced starch-D (526 vs. 694 g/kg starch; p &lt; 0.001), silages produced from medium harvest had higher (p &lt; 0.001) starch content (317 vs. 137 g/kg DM) and higher amount of digestible starch (169 vs. 98.5 g/kg DM; p &lt; 0.001) compared to very early harvest. The 2-way interactions (inoculation × storage time and maturity × storage time) showed that inoculation of corn silage with L. buchneri increased (p &lt; 0.001) the aerobic stability, and that more mature crop silage had higher aerobic stability (140 h; p = 0.036) than the others (118 and 48.5 h for those silages from very early and early harvest).ConclusionThe storage for a longer time (&gt;120 d) with the goal of increasing silage digestibility did not occur. Harvesting whole-crop flint corn with 300 to 350 g/kg DM is desirable to have higher DM yield and starch accumulation. Inoculation with L. buchneri is recommended to preserve the silage against aerobic deterioration. This study has shown the importance of harvesting flint corn at the right time, and the need for inoculation with L. buchneri to ensure greater yield, starch accumulation, and silage preservation, if 120 days of storage are not exceeded.
Formation of low- and high-spatial frequency laser-induced periodic surface structures (LIPSSs) in ALD-deposited MoS2
Authors: Becher Malte J. M. J. , Jagosz Julia , Bock Claudia , Ostendorf Andreas , Gurevich Evgeny L. 
Published: July 2023

The formation of laser-induced periodic surface structures (LIPSSs) on the atomic layer-deposited (ALD) molybdenum disulfide (MoS2) upon femtosecond laser processing is studied experimentally. Laser-processing parameters such as average laser power and the scan speed at which the formation of the periodic nanostructures takes place are identified. Optical and scanning electron microscopy are applied to identify the parameter regions for the different LIPSS formations and transitions between them. High- and low-spatial frequency LIPSS (HSFL and LSFL) with two distinct periods λLSFL ≈ 1.1 μm and λHSFL ≈ 83 nm can be observed. The HSFL are dominating at higher and the LSFL at lower laser average powers. Formation of LIPSS is found to inhibit laser ablation at lower scan speeds.
E-YOLOv4-tiny: a traffic sign detection algorithm for urban road scenarios
Authors: Xiao Yanqiu , Yin Shiao , Cui Guangzhen , Zhang Weili , Yao Lei , Fang Zhanpeng 
Published: July 2023

IntroductionIn urban road scenes, due to the small size of traffic signs and the large amount of surrounding interference information, current methods are difficult to achieve good detection results in the field of unmanned driving.MethodsTo address the aforementioned challenges, this paper proposes an improved E-YOLOv4-tiny based on the YOLOv4-tiny. Firstly, this article constructs an efficient layer aggregation lightweight block with deep separable convolutions to enhance the feature extraction ability of the backbone. Secondly, this paper presents a feature fusion refinement module aimed at fully integrating multi-scale features. Moreover, this module incorporates our proposed efficient coordinate attention for refining interference information during feature transfer. Finally, this article proposes an improved S-RFB to add contextual feature information to the network, further enhancing the accuracy of traffic sign detection.Results and discussionThe method in this paper is tested on the CCTSDB dataset and the Tsinghua-Tencent 100K dataset. The experimental results show that the proposed method outperforms the original YOLOv4-tiny in traffic sign detection with 3.76% and 7.37% improvement in mAP, respectively, and 21% reduction in the number of parameters. Compared with other advanced methods, the method proposed in this paper achieves a better balance between accuracy, real-time performance, and the number of model parameters, which has better application value.