bluuebunny/crossref_metadata_2025_split huggingface dataset:
Rapid In Vitro Screening of Prunus Genotypes for Resistance to Armillaria Root Rot Using Roots of Young Rootstocks
Authors: Devkota Pratima , Iezzoni Amy , Gasic Ksenija , Reighard Gregory , Hammerschmidt Raymond 
Published: September 2023

Armillaria root rot (ARR), caused by Armillaria species and Desarmillaria tabescens, is a severe disease that affects stone fruit trees in the United States. One strategy to mitigate the impact of this disease is to develop ARR-resistant rootstocks. However, current techniques to screen Prunus species for resistance to ARR are time-consuming, labor-intensive, and may not fully replicate field conditions. To address these limitations, we developed a new rapid in vitro screening assay, which uses roots of 2-year-old Prunus rootstock genotypes. We screened 12 Prunus genotypes against Armillaria mellea, Armillaria solidipes, and Desarmillaria tabescens in vitro. Freshly excavated root segments were placed next to or on top of fungal cultures. After 21 days, the circumferential percentage and horizontal length of the fungal colonization and the ability of the fungus to enter through root periderm were evaluated. The root tissue surrounding the infection was also evaluated to assess any response reactions against the ARR pathogens. Our results showed that inoculated root tissues displayed signs of fungal infection, and infection and host responses varied among the Prunus genotypes. Host responses similar to those observed in the field, such as compartmentalization of infected tissue with barrier zones, necrophylactic periderm formation, and callus formation on root surfaces, were observed and were more evident in less susceptible genotypes. In conclusion, our newly developed assay, which uses freshly excavated roots from 2-year-old rootstocks, can rapidly screen Prunus genotypes for resistance to ARR.
A comprehensive method to estimate flood levels of rivers subject to ice jams: A case study of the Chaudière River, Québec, Canada
Authors: Ladouceur Jean-Robert , Morse Brian , Lindenschmidt Karl-Erich 
Published: September 2023

Abstract
The main difference between an open-water (regular) flood and an ice jam flood is that it is normally the whole river length that is overtopped whereas an ice jam flood is localized to where the jam is located. Comparatively, the regular flood analysis can use the value of the extreme discharge as the main input parameter for a long river section, an ice jam flood needs to account for the probability of jams of various lengths and intensities occurring at specific locations under significantly variable discharges while having several mechanical ice parameters to be considered. Through the case study of the Chaudière River, the methodology presented in this paper demonstrates how to statistically characterize four significant inputs (jam location, jam length, jam properties and river discharge during jam event) into the widely used numerical river water model (HEC-RAS) and how Monte–Carlo simulations are generated to estimate probable ice jam floods along a whole river reach. The purpose of this article is to propose a robust methodology through a case study and asses the sensitivity that historical and mechanical parameters have as to why specific locations along the reach have higher 1:100 AEP ice-induced water levels as to 1:100 AEP open-water levels.
Modeled and measured SARS-CoV-2 virus in septic tank systems for wastewater surveillance
Authors: Li Dong , Quon Hunter , Ervin Jared , Jiang Sunny , Rosso Diego , Van De Werfhorst Laurie C. , Steets Brandon , Holden Patricia A. 
Published: September 2023

Abstract

SARS-CoV-2 wastewater surveillance (WWS) at wastewater treatment plants (WWTPs) can reveal sewered community COVID-19 prevalence. For unsewered areas using septic tank systems (STSs) or holding tanks, how to conduct WWS remains unexplored. Here, two large STSs serving Zuma Beach (Malibu, CA) were studied. Supernatant and sludge SARS-CoV-2 concentrations from the directly-sampled STSs parameterized a dynamic solid–liquid separation, mass balance-based model for estimating the infection rate of users. Pumped septage before hauling and upon WWTP disposal was also sampled and assessed. Most (96%) STS sludge samples contained SARS-CoV-2 N1 and N2 genes, with concentrations exceeding the supernatant and increasing with depth while correlating with total suspended solids (TSS). The trucked septage contained N1 and N2 genes which decayed (coefficients: 0.09–0.29 h−1) but remained detectable. Over approximately 5 months starting in December 2020, modeled COVID-19 prevalence estimations among users ranged from 8 to 18%, mirroring a larger metropolitan area for the first 2 months. The approaches herein can inform public health intervention and augment conventional WWS in that: (1) user infection rates for communal holding tanks are estimable and (2) pumped and hauled septage can be assayed to infer where disease is spreading in unsewered areas.
Antibiotic resistance and virulence potentials of E. faecalis and E. faecium in hospital wastewater: a case study in Ardabil, Iran
Authors: Jannati Elham , Khademi Farzad , Manouchehrifar Meysam , Maleki Dadras , Amirmozaffari Nour , Sadat Nikbin Vajihe , Arzanlou Mohsen 
Published: September 2023

Abstract
Hospital wastewater can contaminate the environment with antibiotic-resistant and virulent bacteria. We analyzed wastewater samples from four hospitals in Ardabil province, Iran for Enterococcus faecium and Enterococcus faecalis using culture and molecular methods. We also performed antimicrobial susceptibility testing and polymerase chain reaction testing for resistance and virulence genes. Out of 141 enterococci isolates, 68.8% were E. faecium and 23.4% were E. faecalis. Ciprofloxacin and rifampicin showed the highest level of resistance against E. faecalis and E. faecium isolates at 65%. High-level gentamicin resistance (HLGR), high-level streptomycin resistance (HLSR), ampicillin, and vancomycin resistance were observed in 25, 5, 10, and 5.15% of E. faecium, and 15, 6, 15, and 3.03% of E. faecalis isolates, respectively. The ant(6′)-Ia and ant(3′)-Ia genes that were responsible for streptomycin resistance were observed in HLSR isolates and aph(3′)-IIIa and aac(6′) Ie-aph(2″)-Ia genes accounting for gentamicin resistance were detected in HLGR isolates. vanA was the predominant gene detected in vancomycin-resistant isolates. The majority of isolates were positive for gelE, asa1, esp, cylA, and hyl virulence genes. We found that drug-resistant and virulent E. faecalis and E. faecium isolates were prevalent in hospital wastewater. Proper treatment strategies are required to prevent their dissemination into the environment.
AVALIAÇÃO DO CONHECIMENTO EM EMERGÊNCIAS PSIQUIÁTRICAS ENTRE DISCENTES DE MEDICINA
Authors: Silva Arilson Lima da , Reis Giovana Silva Correa , Assayag Pedro Paulo Cardoso , Pinheiro Estherfanny da Nóbrega , Carreira Luciana Brandão 
Published: September 2023

Introdução: As emergências psiquiátricas (EP) são distúrbios agudos do pensamento, comportamento, humor ou relações sociais, necessitando de uma intervenção imediata. Além disso, ressalta-se que o diagnóstico psiquiátrico de maneira geral difere dos demais por exigir além de habilidades técnico-científicas ou de anamnese uma prática interpessoal e conhecimento do contexto pessoal do paciente. Objetivo: O presente estudo tem como objetivo avaliar o nível de conhecimento dos acadêmicos de medicina de uma universidade pública acerca das emergências psiquiátricas. Metodologia: Trata-se de um estudo observacional, descritivo, do tipo transversal, realizado com 293 estudantes de medicina. Foi aplicado um questionário validado, via Google Forms. Foi utilizado o teste qui-quadrado para avaliar as correlações. Resultados: observou-se que há uma associação entre período do curso e conhecimento acerca de emergências psiquiátricas. Ademais, notou-se escassez de cenários de prática para os acadêmicos. Conclusões: Conclui-se que existem lacunas na abordagem da educação médica sobre o tema em questão e faz-se necessário uma discussão aprofundada para melhoria nesse quesito.
Palavras-Chave: Psiquiatria, Educação médica, Serviços de Emergência Psiquiátrica.
Anterior Chest Wall Non-traumatic Arthropathies: A Crucial but Often
Overlooked Site
Authors: Ben Nessib Dorra , Ferjani Hanene Lassoued , Majdoub Fatma , Ben Aissa Rania , Gzam Yosra , Kaffel Dhia , Maatallah Kaouther , Hamdi Wafa 
Published: September 2023

Objective:
The purpose of this study was to describe the distribution of Anterior Chest
Wall (ACW) arthropathies in a tertiary care center and identify clinical, biological and imaging
findings to differentiate osteoarthritis (OA) from non-osteoarthritis (N-OA) etiologies.

Methods:
Search from medical records from January 2009 to April 2022, including patients with
manubriosternal and/or sternoclavicular and/or sternocostal joint changes confirmed by ultrasonography,
computed tomography or magnetic resonance imaging. The final study group was divided into
OA and N-OA subgroups.

Results:
A total of 108 patients (34 males and 74 females, mean age: 47.3 ± 13 years) were included.
Twenty patients had findings of OA, while 88 were diagnosed with N-OA pathologies. SpA
was the most common etiology in the N-OA group (n = 75). The other N-OA etiologies were less
common: rheumatoid arthritis (n = 4), Synovitis, acne, pustulosis, hyperostosis, osteitis (SAPHO)
syndrome (n = 3), infectious arthritis (n = 3) and microcrystalline arthropathies (n = 3). Regarding
the distinctive features, ACW pain was the inaugural manifestation in 50% of patients in OA group
and 18.2% of patients in N-OA group (p = 0.003); high inflammatory biomarkers were more common
in N-OA group (p = 0.033). Imaging findings significantly associated with OA included subchondral
bone cysts (p < 0.001) and intra-articular vacuum phenomenon (p < 0.001), while the presence
of erosions was significantly associated with N-OA arthropathies (p = 0.019). OA was independently
predicted by the presence of subchondral bone cysts (p = 0.026).

Conclusion:
ACW pain is a common but often underestimated complaint. Knowledge of the different
non-traumatic pathologies and differentiation between OA and N-OA etiologies is fundamental
for appropriate therapeutic management.
Tianeptine Affects the Improvement of Behavioral Defects, such as
Schizophrenia, Caused by Maternal Immune Activation in the Mice Offspring
Authors: Jung Kooseung , Kwon Jun-Tack 
Published: September 2023

Background:
Simultaneously with studies on animal models of fetal-induced maternal immune activation, related studies documented behavior, neurophysiological, and/or neurochemical disorders observed in some neuropsychiatric disorders, including autism and schizophrenia.

Objective:
To investigate whether treatment tianeptine might ameliorate maternal immune activation (MIA)-induced behavioral deficits in the offspring.

Materials and Methods:
The pregnant mice were injected through tail vein injection at a concentration of 5 mg/kg of polyriboinosinic-polyribocytidilic acid (polyI:C) and/or used saline as a vehicle. The injection was performed on the 9th day of pregnancy. Each group of MIA offspring was subjected to vehicle, clozapine, or tianeptine treatment.

Results:
In prepulse inhibition (PPI) test, oral treatment with tianeptine ameliorated MIA-induced sensorimotor gating deficit. Most behavioral parameters of social interaction test (SIT), forced swimming test (FST), and open field test (OFT) were significantly changed in the MIA offspring. Tianeptine treatment significantly recovered behavioral changes observed in the SIT, OFT, and FST. In order to confirm expression level of neurodevelopmental proteins, immunohistochemical image analysis and Western blot were performed, and the medial prefrontal cortex (mPFC) was targeted. As a result, it was confirmed that the neurodevelopmental proteins were decreased, which was recovered after administration of tianeptine to MIA offspring.

Conclusion:
Tianeptine might be useful for treating psychiatric disorders with neurodevelopmental issues.
Cystatin C–Based eGFR Changes during Gender-Affirming Hormone Therapy in Transgender Individuals
Authors: van Eeghen Sarah A. , Wiepjes Chantal M. , T'Sjoen Guy , Nokoff Natalie J. , den Heijer Martin , Bjornstad Petter , van Raalte Daniël H. 
Published: September 2023

Background
Men with CKD tend to experience a faster eGFR decline than women, potentially because of sex hormones. Limited research exists regarding the effect of gender-affirming hormone therapy (GAHT) on kidney function. Furthermore, monitoring kidney function during GAHT is challenging because serum creatinine is confounded by body composition. To investigate the relationship between sex hormones and kidney function, we studied the changes of serum creatinine and serum cystatin C, a filtration marker less affected by sex, during 1 year of GAHT.

Methods
As part of the European Network for the Investigation of Gender Incongruence study, we measured serum creatinine and serum cystatin C in 260 transgender women and 285 transgender men before and 12 months after initiating GAHT. Transgender women received estradiol plus cyproterone acetate, while transgender men received testosterone. Cystatin C–based eGFR was calculated using the full-age-spectrum equation.

Results
In transgender women, cystatin C decreased by 0.069 mg/L (95% confidence interval [CI], 0.049 to 0.089), corresponding with a 7 ml/min per 1.73 m2 increase in eGFR. In transgender men, cystatin C increased by 0.052 mg/L (95% CI, 0.031 to 0.072), corresponding with a 6 ml/min per 1.73 m2 decrease in eGFR. Creatinine concentrations decreased (−0.065 mg/dl; 95% CI, −0.076 to −0.054) in transgender women and increased (+0.131 mg/dl; 95% CI, 0.119 to 0.142) in transgender men. Changes in creatinine-based eGFR varied substantially depending on the sex used in the equation.

Conclusions
In this cohort of transgender individuals, cystatin C–based eGFR increased with estradiol and antiandrogen therapy and decreased with testosterone therapy.
Entre distancias y diferencias: la diversificación social de las desigualdades y la polarización
Authors: Bokser Misses-Liwerant Judit 
Published: September 2023

La necesidad de hacer acopio de esfuerzos para entender nuestro momento, para intentar dar cierta hoja de ruta que ayude a comprender los escenarios posibles del mañana son un irrenunciable estímulo a nuestro quehacer. Es por ello por lo que para la Revista Mexicana de Ciencias Políticas y Sociales resulta fundamental articular un espacio de discusión sobre investigaciones actuales y reinterpretaciones de académicas y académicos sobre el fenómeno de la desigualdad, la jerarquización social y la exclusión. En los artículos que componen el dossier de este número 249 titulado “Polarización, jerarquización y (des)integración: procesos actuales de desigualdad social” se conjuntan temáticas multi e interdisciplinarias que rearticulan la discusión sobre el panorama de polarización que se vive actualmente en México, en nuestra región y en el ámbito global.
EVALUATION OF THE PROPERTIES OF BIOCHAR OBTAINED FROM RICE HUSK FOR ITS APPLICATION IN AGRICULTURAL SOILS
Authors: Souza Débora Machado de , Modolo Regina Célia Espinosa , Santos Emanuele Araújo dos , Silva Jenifer Lima da , Sachetti Felipe Aloísio , Kappler Genyr , Brehm Feliciane Andrade , Moraes Carlos Alberto Mendes 
Published: September 2023

The increasing world population boosted the demand for basic needs such as energy, water, and food. In turn, agricultural production has increased, generating a large amount of agro-industrial waste every year. Globally, Brazil is the seventh-largest rice producer, and the State of Rio Grande do Sul is responsible for 70% of the rice production in Brazil. Rice husks are composed of organic compounds (cellulose, hemicellulose, lignin, and extractives) and inorganic elements that include potassium, calcium, magnesium, and sulfur. These inorganic elements are essential nutrients in the soil. Since natural degradation is slow due to the aromaticity of lignin, rice husk (RH) waste accumulates and poses an environmental threat, causing air and water pollution. In this context, in order to return the biochar to the soil, the properties of rice husk and rice husk biochar were evaluated. Parameters such as pH, true density, bulk density, porosity, identification of the composition and concentration of the elements, analysis of the functional groups, total carbon (TC), and cation exchange capacity (CEC) were performed on RH biochar samples. These samples were produced in a laboratory-scale pyrolysis rig, under a con-trolled atmosphere of Nitrogen (N), using three peak temperatures (350 °C, 450 °C, and 550 °C) and three soak times (30, 60, and 120 min).Based on the results obtained in the characterization analyses of the biochar, as well as the yield values found, it is concluded that the best pyrolysis temperature for the production of biochar from rice husks, for application in soils, is 550 °C.
Validity and reliability of the adapted Dutch version of the Brace Questionnaire (BrQ)
Authors: Peeters Charles M M , Bonsel Joshua M , Munnik-Hagewoud Roelina , Mostert Adriaan K , Van Solinge Guido B , Rutges Joost P H J , Altena Mark C , Krabbe Paul F M , Bos G J F Joyce , Faber Chris , Wapstra Frits-Hein , Kempen Diederik H R 
Published: September 2023

Background and purpose: The Brace Questionnaire (BrQ) is a disease-specific health-related quality of life (HRQOL) instrument for measuring perceived health status of scoliosis patients undergoing brace treatment. The purpose of this study is to evaluate the validity and reliability of a translated and culturally adapted Dutch version of the BrQ.Patients and methods: The original Greek BrQ was translated into Dutch and a cross-cultural adaptation and validation processes were conducted. Subsequently, 80 adolescent idiopathic scoliosis (AIS) patients undergoing active brace treatment were included from 4 scoliosis centers to evaluate the validity and reliability of the Dutch version of the BrQ. The questionnaire’s floor and ceiling effects, internal consistency, and test–retest reliability were assessed. Concurrent validity was evaluated by comparing the BrQ with the revised Scoliosis Research Society 22-item questionnaire (SRS-22r) scores.Results: The mean total BrQ score was 75.9 (standard deviation [SD] 11.3) and the mean domain scores varied between 3.4 (SD 0.9) and 4.2 (SD 0.7) for the domains “vitality” and “bodily pain,” respectively. There were no floor and ceiling effects for the total BrQ score. The BrQ showed satisfactory internal consistency in most subdomains with a Cronbach’s α ranging between 0.35 for the domain “general health perception” and 0.89 for the domain “self-esteem and aesthetics.” Excellent test–retest reproducibility was observed for the total BrQ score (ICC 0.91), and the BrQ was successfully validated against the SRS-22r.Conclusion: The translated and culturally adapted Dutch version of the BrQ is a valid and reliable HRQOL instrument for AIS patients undergoing brace treatment.
importância do ensino religioso na rede de apoio familiar em crianças com Transtorno de Espectro Autista (TEA)
Authors: Jesus Helder Vieira de 
Published: September 2023

Este trabalho trata da questão da disciplina Ensino Religioso como suporte para Rede de Apoio Familiar como mediadora no desenvolvimento de crianças com Transtorno de Espectro Autista (TEA). Diante deste contexto, o presente estudo delimitou como problema de investigação a seguinte questão: Qual a importância da disciplina Ensino Religioso como suporte para a Rede de Apoio Familiar como elemento fundamental na vida de crianças que apresentam Transtorno do Espectro Autista? A fim de responder a estas questões e possibilitar uma compreensão maior da temática, delimitou-se como objetivo geral discutir a importância do Ensino Religioso como suporte na Rede de Apoio Familiar como mediadora no desenvolvimento de crianças que possuem TEA. Entre os objetivos específicos destacam-se: ensino religioso, religiosidade e o transtorno do espectro autista; especificar a Rede de Apoio Familiar na infância; demonstrar como a Rede de Apoio Familiar é relevante na vida das crianças; e apresentar a importância da Rede de Apoio Familiar no tratamento da TEA. Como método, adotou a revisão bibliográfica. O levantamento foi feito através de livros, e em bancos de dados: Scielo (Scientific Eletronic Library OnLine), PePSIC (Portal de Periódicos Eletrônicos de Psicologia), Portal de Periódicos CAPES/MEC e Google Acadêmico. As conclusões mostraram que ao criarem estratégias para contornar as situações difíceis, os familiares podem adquirir a capacidade de superação das adversidades e, assim, terem implicações positivas em sua qualidade de vida.
Cystic Fibrosis Associated Liver Disease and Bronchiectasis in Puberty. Case Report and Literature Review
Authors: Peptisor Cristina-Laura , Arghir Ioan Anton , Ion Irina , Nemes Alexandra Floriana , Mihai Maria-Cristina , Chisnoiu Tatiana , Mosescu Simona , Arghir Oana-Cristina 
Published: September 2023

Abstract
Cystic fibrosis (CF) represents a multisystemic condition, due to the transmembrane conductance regulatory protein (CFTR gene), involving both gastrointestinal tract and lungs. The prevalence of CF associated liver disease is related with increased life expectancy and it is rare in childhood or puberty. Liver disease (LD) is often preceded by complications associated with pulmonary damage. We present a case of a 13 year- old girl, known with cystic fibrosis since the age of 4 months, diagnosed with cholecyst hypoplasia and cholestasis, severe malnutrition progressive extension of bronchiectasis, recurrent respiratory infections in childhood, and, later, in puberty, liver disease with third stage fibrosis (F3) revealed by Fibroscan. Hepatobiliary damage is a late consequence, which is precisely why not performing a liver biopsy, especially in infants and young children, can be justified. We highlight the value of Fibroscan, a non-invasive imaging technique capable to reveal liver fibrosis and to stage it.
Paratesticular Ectopic Adrenal Rests: Case Report and Ancillary Features
Authors: Dinu Alexandra , Burlacu Ionut , Aschie Mariana , Deacu Mariana , Poinareanu Ionut , Iorga Ionut Ciprian , Talpes Raluca , Baltatescu Gabriela-Isabela , Cozaru Georgeta Camelia , Nicolau Antonela-Anca , Cojocaru Oana , Ghitoi Sinziana-Andra , Rosu Mihai-Catalin , Enciu Manuela 
Published: September 2023

Abstract
Ectopic adrenal rests is a rare lesion, with different locations, and more frequently encountered in the pediatric population. This is a case presentation of a 75-years-old male patient, who was hospitalised to the Emergency Department, with altered general condition, pain in the right hemiscrotum and fever. After the orchiectomy, the histopathological examination established the diagnosis of non-specific purulent degenerated cystic type of acute orchiepididymitis and ectopic adrenal tissue. The immunohistochemical examination confirmed the diagnosis of adrenal ectopy. In this case, the ectopic lesion was discovered accidentally, being associated with nonspecific acute inflammation. This is another case in addition to the very rarely reported and the location of testicular adrenal cortical ectopia in adults.