bluuebunny/crossref_metadata_2025_split huggingface dataset:
Whole-Genome Sequencing to Predict Antimicrobial Susceptibility Profiles in Neisseria gonorrhoeae
Authors: Bristow Claire C , Mortimer Tatum D , Morris Sheldon , Grad Yonatan H , Soge Olusegun O , Wakatake Erika , Pascual Rushlenne , Murphy Sara McCurdy , Fryling Kyra E , Adamson Paul C , Dillon Jo-Anne , Parmar Nidhi R , Le Hai Ha Long , Van Le Hung , Ovalles Ureña Reyna Margarita , Mitchev Nireshni , Mlisana Koleka , Wi Teodora , Dickson Samuel P , Klausner Jeffrey D 
Published: February 2023

Abstract

Background
Neisseria gonorrhoeae is a major public health problem due to increasing incidence and antimicrobial resistance. Genetic markers of reduced susceptibility have been identified; the extent to which those are representative of global antimicrobial resistance is unknown. We evaluated the performance of whole-genome sequencing (WGS) used to predict susceptibility to ciprofloxacin and other antimicrobials using a global collection of N. gonorrhoeae isolates.

Methods
Susceptibility testing of common antimicrobials and the recently developed zolifodacin was performed using agar dilution to determine minimum inhibitory concentrations (MICs). We identified resistance alleles at loci known to contribute to antimicrobial resistance in N. gonorrhoeae from WGS data. We tested the ability of each locus to predict antimicrobial susceptibility.

Results
A total of 481 N. gonorrhoeae isolates, collected between 2004 and 2019 and making up 457 unique genomes, were sourced from 5 countries. All isolates with demonstrated susceptibility to ciprofloxacin (MIC ≤0.06 μg/mL) had a wild-type gyrA codon 91. Multilocus approaches were needed to predict susceptibility to other antimicrobials. All isolates were susceptible to zoliflodacin, defined by an MIC ≤0.25 μg/mL.

Conclusions
Single marker prediction can be used to inform ciprofloxacin treatment of N. gonorrhoeae infection. A combination of molecular markers may be needed to determine susceptibility for other antimicrobials.
The Effect of Burns on Children’s Growth Trajectory: A Nationwide Cohort Study
Authors: Cuijpers Maxime D , van de Sande Pauline J H , Cords Charlotte I , Scholten-Jaegers Sonja M H J , van Zuijlen Paul P M , Baartmans Martin G A , Pijpe Anouk 
Published: February 2023

Abstract
This study evaluates the short- and long-term effect of burns on children’s height and weight, by comparing their pre and postburn growth trajectory. We invited children (≤17 years old), who sustained a burn requiring surgical treatment or admission at one of the Dutch burn centers in 2013 (n = 175). As well as children who sustained a severe burn, covering &gt;10% of the total body surface area (TBSA), throughout 2009–2018 (n = 228). Data was collected from a survey on health-related topics, Youth Health Care records, and the Dutch Burn Repository R3. For all participants, height and weight were converted to Z-scores using Dutch reference values. Linear mixed modeling, nested on the individual level, was used to examine the associations between burns and children’s height and weight Z-scores. Children’s height and weight Z-scores remained within the normal range throughout the study period. During the first-year postburn, children’s height and weight Z-scores decreased by −0.21 (95% CI −0.41, −0.01) and −0.23 (95% CI −0.46, −0.04), respectively. Beyond the first-year postburn, estimates were consistent with a positive linear association between burn size and the overall effect of burns on participants’ height and weight Z-scores. This included a modest, but statistically significant, effect among participants with a burn covering ≤4.5% and &gt;14.0% of the TBSA. Sensitivity analyses did not alter our findings. In conclusion, children were on track or even surpassed their growth potential. Our findings could therefore be considered reassuring to patients, parents, and clinicians.
Vibration analysis of electric motors considering rotating rotor structure using flexible multibody dynamics-electromagnetic-structural vibration coupled analysis
Authors: Cho Seunghyeon , Jeon Kyunghun , Kim Chang-Wan 
Published: February 2023

AbstractIn this study, we develop flexible multibody dynamic-electromagnetic-structural vibration coupled analysis method to accurately predict motor vibration by considering the electromagnetic force characteristics, rotating characteristics of rotating motor motors, and their interactions at the no-load rated speed and operating speed range. The structural characteristics are accurately reflected by developing a three-dimensional (3D) finite element model considering the entire components of the motor. The reliability of the 3D finite element model of the motor is verified using the impact hammer test. In addition, to consider the rotational characteristics of the rotor structure, we develop a flexible multibody dynamics model that connects the flexible rotor and the bearing with revolute joint. The vibration of the motor at the no-load rated speed is analyzed using flexible multibody dynamics-electromagnetic-structural vibration coupled analysis. Comparing the vibration test results, it is confirmed that the flexible multibody dynamics-electromagnetic-structural vibration coupled analysis result predicts the actual motor vibration more accurately than the conventional finite element analysis-based electromagnetic-structural vibration coupled analysis result. By using flexible multibody dynamics-electromagnetic-structural vibration coupled analysis in the operating speed range, it is confirmed that not only electromagnetic force harmonics but also sideband harmonics caused by rotor eccentricity-induced large vibrations, and also confirmed that it accurately predicts the vibration characteristics of actual motors with rotating rotors.
The Effects of Government Spending in the Eurozone
Authors: Gabriel Ricardo Duque , Klein Mathias , Pessoa Ana Sofia 
Published: February 2023

Abstract
Using a newly assembled rich dataset at the regional level, this paper provides novel empirical evidence on the effects of fiscal policy in the Eurozone. Our baseline estimates reveal a government spending relative output multiplier around 2, an employment multiplier of 1.4, and a cost per job created of approximately €30,000. Moreover, we find that a regional fiscal stimulus leads to a significant increase in private investment, productivity, and durable consumption. The labor share also increases, as do total hours worked driven by changes in the extensive margin (total employment), whereas the intensive margin (hours per worker) barely reacts. Contrary to the common policy narrative of strong positive spillover effects, we estimate only small regional fiscal spillovers. Finally, our findings reveal strong heterogeneities across economic sectors, states of the economy, and member states.
Effects of Taurine as Dietary Supplement on the Biological and Demographic Parameters ofNezara viridula(Heteroptera: Pentatomidae)
Authors: Rojas M Guadalupe , Morales-Ramos Juan A 
Published: February 2023

AbstractThe southern green stink bug, Nezara viridula (L.) (Heteroptera: Pentatomidae), is a widely distributed pest of many economically important crops. Because of its economic impact, multiple examples of rearing methods and diets for N. viridula have been published. However, rearing this pest year-round consistently in all-vegetable diets has been challenging. Preliminary observations have shown that supplementing N. viridula diet with insect components improves the survival and reproduction of this insect. We hypothesized that taurine could be the nutrient present in insect components that was providing the benefits. Treatments consisting of three different watering regimes: 1) Reverse osmosis (RO) water only (W), 2) 2% taurine solution only (T), and 3) a choice between RO water and 2% taurine solution (T&W) were compared for their effects on life cycle and demographic parameters of N. viridula. Both taurine-containing treatments (T and T&W) resulted in a significant increase in nymphal and premating adult survival and egg viability as compared with treatment ‘W’. Taurine supplementation did not have significant effect on fecundity and development time significantly increased in the ‘T’ treatment compared with W and W&T treatments. However, there were significant improvements in demographic parameters showing an increase in fitness levels after taurine supplementation. These results suggest that taurine is an important nutrient for N. viridula, which has been deficient in traditional diets consisting exclusively of vegetable components. Adoption of this new information will help to improve the survival of N. viridula in culture to facilitate this study to develop new methods for its control.
Odorant-binding Protein 10 From Bradysia odoriphaga (Diptera: Sciaridae) Binds Volatile Host Plant Compounds
Authors: Zhu Jiaqi , Wang Fu , Zhang Youjun , Yang Yuting , Hua Dengke 
Published: February 2023

Abstract
Bradysia odoriphaga (Diptera: Sciaridae) is a major insect pest of seven plant families including 30 commercial crops in Asia. The long-term use of chemical pesticides leads to problems such as insect resistance, environmental issues, and food contamination. Against this background, a novel pest control method should be developed. In insects, odorant-binding proteins (OBPs) transport odor molecules, including pheromones and plant volatiles, to olfactory receptors. Here, we expressed and characterized the recombinant B. odoriphaga OBP BodoOBP10, observing that it could bind the sulfur-containing compounds diallyl disulfide and methyl allyl disulfide with Ki values of 8.01 μM and 7.00 μM, respectively. Homology modeling showed that the BodoOBP10 3D structure was similar to that of a typical OBP. Both diallyl disulfide and methyl allyl disulfide bound to the same site on BodoOBP10, mediated by interactions with six hydrophobic residues Met70, Ile75, Thr89, Met90, Leu93, and Leu94, and one aromatic residue, Phe143. Furthermore, silencing BodoOBP10 expression via RNAi significantly reduced the electroantennogram (EAG) response to diallyl disulfide and methyl allyl disulfide. These findings suggest that BodoOBP10 should be involved in the recognition and localization of host plants.
Trans-epithelial migration is essential for neutrophil activation during RSV infection
Authors: Robinson Elisabeth , Herbert Jenny Amanda , Palor Machaela , Ren Luo , Larken Isobel , Patel Alisha , Moulding Dale , Cortina-Borja Mario , Smyth Rosalind Louise , Smith Claire Mary 
Published: February 2023

Abstract
The recruitment of neutrophils to the infected airway occurs early following respiratory syncytial virus (RSV) infection, and high numbers of activated neutrophils in the airway and blood are associated with the development of severe disease. The aim of this study was to investigate whether trans-epithelial migration is sufficient and necessary for neutrophil activation during RSV infection.
Here, we used flow cytometry and novel live-cell fluorescent microscopy to track neutrophil movement during trans-epithelial migration and measure the expression of key activation markers in a human model of RSV infection. We found that when migration occurred, neutrophil expression of CD11b, CD62L, CD64, NE, and MPO increased. However, the same increase did not occur on basolateral neutrophils when neutrophils were prevented from migrating, suggesting that activated neutrophils reverse migrate from the airway to the bloodstream side, as has been suggested by clinical observations. We then combined our findings with the temporal and spatial profiling and suggest 3 initial phases of neutrophil recruitment and behavior in the airways during RSV infection; (1) initial chemotaxis; (2) neutrophil activation and reverse migration; and (3) amplified chemotaxis and clustering, all of which occur within 20 min. This work and the novel outputs could be used to develop therapeutics and provide new insight into how neutrophil activation and a dysregulated neutrophil response to RSV mediates disease severity.
Transient Viral Rebound in Children with Perinatally Acquired HIV-1 Induces a Unique Soluble Immunometabolic Signature Associated with Decreased CD4/CD8 Ratio
Authors: Tarancon-Diez Laura , Peraire Joaquim , Jiménez de Ory Santiago , Guirro Maria , Escosa Luis , Prieto Tato Luis Manuel , Penín Antón María , Piqueras Ana Isabel , Vázquez Pérez Álvaro , Gavilán César , Bustillo-Alonso Matilde , Navarro María Luisa , Viladés Consuelo , Vidal Francesc , Rull Anna , Muñoz-Fernández María Ángeles 
Published: February 2023

Abstract

Background
To determine by multi-omic analysis changes in metabolites, lipids, and proteins as a consequence of transient viral rebound (tVR) in children with perinatally acquired HIV-1 (PHIV).

Methods
Plasma samples from children with PHIV and with tVR (first episode of transient RNA-HIV viral load &gt;20 copies/ml followed by suppression) on the time-point immediately before (pre-tVR) and after (post-tVR) the tVR were assessed. Multi-omic analyses were performed using nLC-Orbitrap, GC-qTOF-MS, and LC-qTOF-MS.

Results
Comparing pre- and post-tVR time-points, HIV-1 children with tVR (n = 5) showed a trend to a decrease in ratio CD4/CD8 (p = 0.08) but no significant differences were observed in plasma metabolites, lipids, or proteins. Post-tVR condition was compared with a reference group of children with PHIV with persistent viral control (n = 9), paired by sex, age, and time under antiretroviral treatment. A total of 10 proteins, 8 metabolites, and 2 lipids showed significant differences (p &lt; 0.05): serotransferrin, clusterin, kininogen-1, succinic acid, threonine, 2-hydroxyisovaleric acid, methionine, 2-hydroxyglutaric, triacylglyceride 50:0 (TG50:0), and diacylglyceride 34:1 (DG34:1) were upregulated while alpha-2-macroglobulin, apolipoprotein A-II, carboxylic ester hydrolase, apolipoprotein D, coagulation factor IX, peptidase inhibitor 16, SAA2-SAA4 readthrough, oleic acid, palmitoleic acid, and D-sucrose downregulated on post-tVR time-point compared to the reference group. Ratio CD4/CD8 correlated with apolipoprotein A-II, DG34:1, and methionine (p = 0.004; ρ = 0.71, p = 0.016; ρ = −0.63; and p = 0.032; ρ = −0.57, respectively). Nadir CD4+ correlated inversely with kininogen-1 (p = 0.022; ρ = −0.60) and positively with D-sucrose (p = 0.001; ρ = 0.77).

Conclusions
tVR followed by suppression implies changes in soluble proteins, lipids, and metabolites that correlate with immunological parameters, mainly ratio CD4/CD8, that decreased after tVR. These distinct soluble biomarkers could be considered potential biomarkers of immune progression.
Short-Term (6 Weeks) Experience of a Modular Workcell for Hemostasis Testing Including an Intelligent Data Manager at a Tertiary Care Hospital
Authors: Lee Hwan Tae , Lee So Young , Seo Ja Young , Ahn Jeong-Yeal 
Published: February 2023

Abstract

Objective
Modular workcells could be a better solution than total laboratory automation (TLA) in hemostasis laboratories. Here, we evaluated the impact of implementing a modular workcell (HemoCell) with an intelligent data management facility (HemoHub).

Methods
We compared the turnaround times (TATs), numbers of rerun samples, and rerun times pre- and postimplementation of the HemoCell at Gil Medical Center. Prothrombin time (PT), activated partial thromboplastin time (aPTT), D-dimer, and fibrinogen were evaluated.

Results
The TAT standard deviations (SDs) and maximum TAT values decreased after HemoCell implementation, although the mean TATs for PT, aPTT, and D-dimer were increased. Numbers of rerun samples were increased (18.1/day vs 44.7/day). However, rerun times were reduced, and SDs were decreased during the post-HemoCell period compared with pre-HemoCell. Additionally, technologists needed smaller working space and less labor.

Conclusion
The modular workcell could improve quality and efficiency by providing more consistent TATs and shorter rerun times in the hemostasis laboratory.
Occupational Exposure to Nonionizing Radiation and Risk for Malignancy in Young Adults
Authors: Shapira Shachar , Nitecki Maya , Tzur Dorit , Schwartz Naama , Silverman Barbara G , Zack Oren , Friedensohn-Zuck Limor 
Published: February 2023

ABSTRACT

Introduction
Nonionizing radiation (NIR) is considered “possibly carcinogenic to humans,” and therefore, exposure of young military personnel raises concerns regarding increased risk for cancer. The aim of our study was to compare the cancer incidence in exposed and nonexposed populations in order to gain better understanding of their risk.

Materials and Methods
A longitudinal retrospective cohort study, between 2009 and 2018, was conducted. Israel Defense Forces (IDF) aerial defense units service members, with NIR exposure (range of 2-300 GHz, below the International Commission of Non-Ionizing Radiation Protection guidelines), were compared with a similar sociodemographic group of service members without NIR exposure. Both groups were followed for cancer incidence (all-cause and specific malignancies). Kaplan–Meier analysis of cancer-free survival and univariate and multivariable logistic regressions for possible confounders and risk factors were performed. This analysis was repeated on a matched 1:1 control group.

Results
Exposure and comparison groups included 3,825 and 11,049 individuals, respectively. Forty-one cases diagnosed with cancer were identified during the follow-up time (mean 4.8 [±2.7] years), 13 (0.34%) of which were reported in the exposure group, and 28 (0.25%) were reported in the comparison group. The odds ratio (OR) for cancer incidence in the exposure vs. control groups was 1.34 (95%CI, 0.70-2.60), P-value = 0.3807. The results remained unchanged after adjustment for sex, age at enrollment, service length, socioeconomic status, and military occupation (adjOR = 1.38 [95%CI, 0.67-2.82], P = 0.3818).

Conclusions
Our study did not find an increased short-term risk for cancer in young adults exposed to NIR radiation as compared with unexposed young adults.
VFTS 243 as predicted by the BPASS fiducial models
Authors: Stevance H F , Ghodla S , Richards S , Eldridge J J , Briel M M , Tang P 
Published: February 2023

ABSTRACTThe recent discovery of an unambiguous quiescent black hole (BH) and main-sequence O star companion in VFTS 243 opens the door to new constraints on theoretical stellar evolution and population models looking to reproduce the progenitors of BH–BH binaries. Here, we show that the binary population and spectral synthesis fiducial models natively predict VFTS 243-like systems: We find that VFTS 243 likely originated from a binary system in an ∼15 d orbit with primary mass ranging from 40 to 50 $\mathrm{\, M}_\odot$ and secondary star with initial mass of 24–25 $\mathrm{\, M}_\odot$. Additionally, we find that the death of the primary star must have resulted in a low-energy explosion E &lt; 1050 erg. With a uniform prior, we find that the kick velocity of the newborn BH was ≤10 $\, \text{km s}^{-1}$. The very low eccentricity reported for VFTS 243 and the subsequent conclusion by the authors that the supernova kick must have been very small are in line with the peak in the posterior distribution between 0 and 5 $\, \text{km s}^{-1}$ found from our numerical simulations performed with a uniform prior. Finally, the reduced Hobbs kick distribution commonly used in BH population synthesis is strongly disfavoured.
Multimessenger emission from tidal waves in neutron star oceans
Authors: Sullivan Andrew G , Alves Lucas M B , Spence Georgina O , Leite Isabella P , Veske Doğa , Bartos Imre , Márka Zsuzsa , Márka Szabolcs 
Published: February 2023

ABSTRACT
Neutron stars in astrophysical binary systems represent exciting sources for multimessenger astrophysics. A potential source of electromagnetic transients from compact binary systems is the neutron star ocean, the external fluid layer encasing a neutron star. We present a groundwork study into tidal waves in neutron star oceans and their consequences. Specifically, we investigate how oscillation modes in neutron star oceans can be tidally excited during compact binary inspirals and parabolic encounters. We find that neutron star oceans can sustain tidal waves with frequencies between 0.01 and 20 Hz. Our results suggest that tidally resonant neutron star ocean waves may serve as a never-before studied source of precursor electromagnetic emission prior to neutron star–black hole and binary neutron star mergers. If accompanied by electromagnetic flares, tidally resonant neutron star ocean waves, whose energy budget can reach 1046 erg, may serve as early warning signs (≳1 min before merger) for compact binary mergers. Similarly, excited ocean tidal waves will coincide with neutron star parabolic encounters. Depending on the neutron star ocean model and a flare emission scenario, tidally resonant ocean flares may be detectable by Fermi and Nuclear Spectroscopic Telescope Array (NuSTAR) out to ≳100 Mpc with detection rates as high as ∼7 yr−1 for binary neutron stars and ∼0.6 yr−1 for neutron star–black hole binaries. Observations of emission from neutron star ocean tidal waves along with gravitational waves will provide insight into the equation of state at the neutron star surface, the composition of neutron star oceans and crusts, and neutron star geophysics.
Continuous gravitational wave detection to understand the generation mechanism of fast radio bursts
Authors: Kalita Surajit , Weltman Amanda 
Published: February 2023

ABSTRACT
Since the unexpected discovery of fast radio bursts (FRBs), researchers have proposed varied theories and models to explain these phenomena. One such model that has recently been developed incorporates the so-called Gertsenshtein–Zel’dovich (GZ) effect, which states that when gravitational waves traverse a pulsar magnetosphere, a portion of the gravitational radiation is transformed into electromagnetic (EM) radiation. The observed properties of FRBs are consistent with the properties of this EM radiation, implying, remarkably, that the GZ effect can account for both repeating and non-repeating FRBs. If this model is correct, the pulsar’s properties should not change over time, and it would continue to emit both EM dipole and gravitational quadrupole radiation for a long period of time. This article targets the gravitational radiation produced by the pulsar mechanism and shows that several proposed gravitational wave detectors can detect these gravitational waves. If such detections are performed in the future from the location of FRBs, it might validate the GZ process for FRB production and potentially rule out several other theories of FRB generation.