bluuebunny/crossref_metadata_2025_split huggingface dataset:
Promoting creative autonomy support in school music education: An intervention study targeting interaction
Authors: Hendriks Linda H. , Steenbeek Henderien W. , Bisschop Boele Evert H. , van Geert Paul L. C. 
Published: February 2023

IntroductionThe notion of autonomy in Self-Determination Theory is at the core of intrinsically motivated learning, and fulfilment of the need for autonomy is essential for thriving at school. Therefore teacher-provided autonomy support has grown into a key concern in educational research. In the present study into primary school music education, the notion of creative autonomy support is introduced. Research into autonomy support is typically focused on verbal interaction. However, from an enactive perspective, teachers’ gesturing, bodily movement, facial expression, and musical action form an integral part of the socially situated interaction in music lessons, inherently involving autonomy support. In the present study, a distinction is made between creative verbal autonomy support and creative musical and non-verbal autonomy support.MethodsApplying a process-based time-serial methodology, rooted in a Complex Dynamic Systems and Enactive perspective, the effects of an intervention with Video Feedback Coaching for teachers were investigated. Video data of 105 music lessons of 18 teachers (intervention and control condition) from six primary schools was gathered, to examine teachers’ creative autonomy support at both the individual and group level.ResultsThe findings show that teachers in the intervention condition, compared to the control group, achieved a meaningful increase in their ability to offer creative autonomy support verbally. Teachers also showed development for the non-verbal and musical aspects of offering creative autonomy support. However, particularly for offering higher-level creative autonomy support in the non-verbal and musical mode, significant results were found for less than half of the intervention teachers.DiscussionThese results underline the importance of embracing and studying the bodily dimension as an integral part of teacher autonomy support, aimed at emergence of students’ musical creativity, in primary school music education and in teacher training. We explain how these results might be relevant for autonomy enhancing musical activities in vulnerable groups.
Optimal control of renewable energy communities with controllable assets
Authors: Aittahar Samy , de Villena Miguel Manuel , Derval Guillaume , Castronovo Michael , Boukas Ioannis , Gemine Quentin , Ernst Damien 
Published: February 2023

Introduction: The control of Renewable Energy Communities (REC) with controllable assets (e.g., batteries) can be formalised as an optimal control problem. This paper proposes a generic formulation for such a problem whereby the electricity generated by the community members is redistributed using repartition keys. These keys represent the fraction of the surplus of local electricity production (i.e., electricity generated within the community but not consumed by any community member) to be allocated to each community member. This formalisation enables us to jointly optimise the controllable assets and the repartition keys, minimising the combined total value of the electricity bills of the members.Methods: To perform this optimisation, we propose two algorithms aimed at solving an optimal open-loop control problem in a receding horizon fashion. Moreover, we also propose another approximated algorithm which only optimises the controllable assets (as opposed to optimising both controllable assets and repartition keys). We test these algorithms on Renewable Energy Communities control problems constructed from synthetic data, inspired from a real-life case of REC.Results: Our results show that the combined total value of the electricity bills of the members is greatly reduced when simultaneously optimising the controllable assets and the repartition keys (i.e., the first two algorithms proposed).Discussion: These findings strongly advocate the need for algorithms that adopt a more holistic standpoint when it comes to controlling energy systems such as renewable energy communities, co-optimising or jointly optimising them from both a traditional (very granular) control standpoint and a larger economic perspective.
Smoking, alcohol consumption, and frailty: A Mendelian randomization study
Authors: Lv Jiannan , Wu Lianghua , Sun Sheng , Yu Huifang , Shen Zekai , Xu Jun , Zhu Jiahao , Chen Dingwan , Jiang Minmin 
Published: February 2023

Background: Tobacco smoking and alcohol consumption have been associated with frailty in observational studies. We sought to examine whether these associations reflect causality using the two-sample Mendelian randomization (MR) design.Methods: We used summary genome-wide association statistics for smoking initiation (N = 2,669,029), alcohol consumption (N = 2,428,851), and the frailty index (FI, N = 175,226) in participants of European ancestry. Both univariable and multivariable MR were performed to comprehensively evaluate the independent effects of smoking and alcohol consumption on the FI, accompanied by multiple sensitivity analyses. Results were verified using lifetime smoking and alcohol use disorder. Reverse direction MR was undertaken to assess the potential for reverse causation.Results: Genetic predisposition to smoking initiation was significantly associated with increased FI (univariable MR: β = 0.345; 95% confidence interval [CI] = 0.316 to 0.374; p = 1.36E-113; multivariable MR: β = 0.219; 95% CI = 0.197 to 0.241; p = 2.44E-83). Genetically predicted alcohol consumption showed a suggestive association with the FI (univariable MR: β = −0.090; 95% CI = −0.151 to −0.029; p = 0.003; multivariable MR β = −0.153; 95% CI = −0.212 to −0.094; p = 2.03E-07), with inconsistent results in sensitivity analyses. In complementary analysis, genetic predicted lifetime smoking, but not alcohol use disorder was associated with the FI. There is no convincing evidence for reverse causation.Conclusion: The present MR study supported smoking as a causal risk factor of frailty. Further research is warranted to investigate whether alcohol consumption has a causal role in frailty.
Identification and verification of m7G-Related genes as biomarkers for prognosis of sarcoma
Authors: Qin Haotian , Sheng Weibei , Weng Jian , Li Guoqing , Chen Yingqi , Zhu Yuanchao , Wang Qichang , Chen Yixiao , Yang Qi , Yu Fei , Zeng Hui , Xiong Ao 
Published: February 2023

Background: Increasing evidence indicates a crucial role for N7-methylguanosine (m7G) methylation modification in human disease development, particularly cancer, and aberrant m7G levels are closely associated with tumorigenesis and progression via regulation of the expression of multiple oncogenes and tumor suppressor genes. However, the role of m7G in sarcomas (SARC) has not been adequately evaluated.Materials and methods: Transcriptome and clinical data were gathered from the TCGA database for this study. Normal and SARC groups were compared for the expression of m7G-related genes (m7GRGs). The expression of m7GRGs was verified using real-time quantitative PCR (RT-qPCR) in SARC cell lines. Then, differentially expressed genes (DEGs) were identified between high and low m7GRGs expression groups in SARC samples, and GO enrichment and KEGG pathways were evaluated. Next, prognostic values of m7GRGs were evaluated by Cox regression analysis. Subsequently, a prognostic model was constructed using m7GRGs with good prognostic values by Lasso regression analysis. Besides, the relationships between prognostic m7GRGs and immune infiltration, clinical features, cuproptosis-related genes, and antitumor drugs were investigated in patients with SARC. Finally, a ceRNA regulatory network based on m7GRGs was constructed.Results: The expression of ten m7GRGs was higher in the SARC group than in the control group. DEGs across groups with high and low m7GRGs expression were enriched for adhesion sites and cGMP-PKG. Besides, we constructed a prognostic model that consists of EIF4A1, EIF4G3, NCBP1, and WDR4 m7GRGs for predicting the survival likelihood of sarcoma patients. And the elevated expression of these four prognostic m7GRGs was substantially associated with poor prognosis and elevated expression in SARC cell lines. Moreover, we discovered that these four m7GRGs expressions were negatively correlated with CD4+ T cell levels, dendritic cell level and tumor purity, and positively correlated with tumor mutational burden, microsatellite instability, drug sensitivity and cuproptosis-related genes in patients with sarcomas. Then, a triple regulatory network of mRNA, miRNA, and lncRNA was established.Conclusion: The current study identified EIF4A1, EIF4G3, NCBP1, and WDR4 as prognostic genes for SARC that are associated with m7G.These findings extend our knowledge of m7G methylation in SARC and may guide the development of innovative treatment options.
Gene expression signature of human neuropathic pain identified through transcriptome analysis
Authors: Hu Ling , Yin Wei , Ma Yao , Zhang Qiushi , Xu Qingbang 
Published: February 2023

Introduction: Neuropathic pain is a type of chronic pain that is characterized by ongoing discomfort and can be challenging to manage effectively. This study aimed to identify genes associated with neuropathic pain through transcriptome analysis in order to gain a better understanding of the mechanisms underlying this chronic, difficult-to-treat pain.Methods: We conducted transcriptome analysis using a training datasetof 202 individuals, including patients with neuropathic pain and healthy controls.Results: Our analysis identified five genes (GTF2H2, KLHL5, LRRC37A4P, PRR24, and MRPL23) that were significantly differentially expressed in the tissue of patients with neuropathic pain compared to controls. We constructed a neuropathic pain signature using these five genes and validated it using an independent dataset of 25 individuals. Receiver operating characteristic (ROC) curve analysis demonstrated that this signature had a high level of accuracy in differentiating between neuropathic pain patients and healthy controls, with an area under the curve (AUC) of 0.83 (95% CI 0.65–1).Discussion: These findings suggest that these five genes may be potential therapeutic targets for neuropathic pain.
Determinants of B-Cell Compartment Hyperactivation in European Adolescents Living With Perinatally Acquired HIV-1 After Over 10 Years of Suppressive Therapy
Authors: Ruggiero Alessandra , Pascucci Giuseppe Rubens , Cotugno Nicola , Domínguez-Rodríguez Sara , Rinaldi Stefano , Tagarro Alfredo , Rojo Pablo , Foster Caroline , Bamford Alasdair , De Rossi Anita , Nastouli Eleni , Klein Nigel , Morrocchi Elena , Fatou Benoit , Smolen Kinga K. , Ozonoff Al , Di Pastena Michela , Luzuriaga Katherine , Steen Hanno , Giaquinto Carlo , Goulder Philip , Rossi Paolo , Levy Ofer , Pahwa Savita , Palma Paolo ,   the EPIICAL Consortium
Published: February 2023

BackgroundDespite a successful antiretroviral therapy (ART), adolescents living with perinatally acquired HIV (PHIV) experience signs of B-cell hyperactivation with expansion of ‘namely’ atypical B-cell phenotypes, including double negative (CD27-IgD-) and termed age associated (ABCs) B-cells (T-bet+CD11c+), which may result in reduced cell functionality, including loss of vaccine-induced immunological memory and higher risk of developing B-cells associated tumors. In this context, perinatally HIV infected children (PHIV) deserve particular attention, given their life-long exposure to chronic immune activation.MethodsWe studied 40 PHIV who started treatment by the 2nd year of life and maintained virological suppression for 13.5 years, with 5/40 patients experiencing transient elevation of the HIV-1 load in the plasma (Spike). We applied a multi-disciplinary approach including immunological B and T cell phenotype, plasma proteomics analysis, and serum level of anti-measles antibodies as functional correlates of vaccine-induced immunity.ResultsPhenotypic signs of B cell hyperactivation were elevated in subjects starting ART later (%DN T-bet+CD11c+ p=0.03; %AM T-bet+CD11c+ p=0.02) and were associated with detectable cell-associated HIV-1 RNA (%AM T-bet+CD11c+ p=0.0003) and transient elevation of the plasma viral load (spike). Furthermore, B-cell hyperactivation appeared to be present in individuals with higher frequency of exhausted T-cells, in particular: %CD4 TIGIT+ were associated with %DN (p=0.008), %DN T-bet+CD11c+ (p=0.0002) and %AM T-bet+CD11c+ (p=0.002) and %CD4 PD-1 were associated with %DN (p=0.048), %DN T-bet+CD11c+ (p=0.039) and %AM T-bet+CD11c+ (p=0.006). The proteomic analysis revealed that subjects with expansion of these atypical B-cells and exhausted T-cells had enrichment of proteins involved in immune inflammation and complement activation pathways. Furthermore, we observed that higher levels of ABCs were associated a reduced capacity to maintain vaccine-induced antibody immunity against measles (%B-cells CD19+CD10- T-bet+, p=0.035).ConclusionWe identified that the levels of hyperactivated B cell subsets were strongly affected by time of ART start and associated with clinical, viral, cellular and plasma soluble markers. Furthermore, the expansion of ABCs also had a direct impact on the capacity to develop antibodies response following routine vaccination.
NOX2 inhibition enables retention of the circadian clock in BV2 microglia and primary macrophages
Authors: Muthukumarasamy Iswarya , Buel Sharleen M. , Hurley Jennifer M. , Dordick Jonathan S. 
Published: February 2023

IntroductionSustained neuroinflammation is a major contributor to the progression of neurodegenerative diseases such as Alzheimer’s (AD) and Parkinson’s (PD) diseases. Neuroinflammation, like other cellular processes, is affected by the circadian clock. Microglia, the resident immune cells in the brain, act as major contributors to neuroinflammation and are under the influence of the circadian clock. Microglial responses such as activation, recruitment, and cytokine expression are rhythmic in their response to various stimuli. While the link between circadian rhythms and neuroinflammation is clear, significant gaps remain in our understanding of this complex relationship. To gain a greater understanding of this relationship, the interaction between the microglial circadian clock and the enzyme NADPH Oxidase Isoform 2 (NOX2) was studied; NOX2 is essential for the production of reactive oxygen species (ROS) in oxidative stress, an integral characteristic of neuroinflammation.MethodsBV2 microglia were examined over circadian time, demonstrating oscillations of the clock genes Per2 and Bmal1 and the NOX2 subunits gp91phox and p47phox.ResultsThe BV2 microglial clock exerted significant control over NOX2 expression and inhibition of NOX2 enabled the microglia to retain a functional circadian clock while reducing levels of ROS and inflammatory cytokines. These trends were mirrored in mouse bone marrow-derived primary macrophages.ConclusionsNOX2 plays a crucial role in the interaction between the circadian clock and the activation of microglia/macrophages into their pro-inflammatory state, which has important implications in the control of neuroinflammation.
RNA sequencing provides insights into the effect of dietary ingestion of microplastics and cadmium in the sea cucumber Apostichopus japonicus
Authors: Zhang Chenxi , Zhang Libin , Li Lingling , Mohsen Mohamed , Su Fang , Wang Xu , Lin Chenggang 
Published: February 2023

IntroductionMicroplastics (MPs) and cadmium (Cd) are persistent pollutants in aquatic environments. Sea cucumbers are susceptible to MPs and Cd due to their feeding behavior.MethodsThis study, based on Illumina sequencing, compared the transcriptomes of A. japonicus before and after Cd and/or MPs exposure. Additionally, we detected the changes of catalase (CAT), and superoxide dismutase (SOD) activity, glutathione (GSH), and malondialdehyde (MDA) content in sea cucumbers.Results and DiscussionHigh concentration of MPs caused the increase of SOD activity. High concentration combined treatment resulted in significant up regulation of these four indicators in A. japonicus and had the largest number of differential expression genes (DEGs) reaching 1,618 DEGs, consisting of 789 up regulated along with 829 down regulated DEGs. Transcriptome results showed that Cd induced up regulation of intestinal FAS associated death domain protein (FADD) expression, which may cause apoptosis and inflammation. The increase of intestinal putative heparan sulfate 2-O-sulfotransferase in cadmium treatment groups provided a mechanism for host defense. The imbalance of expression of the NOD-like receptor (NLR) family inflammatory bodies and caspase 6 in the microplastic treatment group also led to the inflammatory reaction in the intestine of sea cucumber. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis showed that in the process of fatty acid metabolism, MPs and Cd showed antagonistic effects, mainly in the inconsistent expression of Stearoyl CoA Ddesaturase (SCD1) protein. The significant changes of Toll interacting protein (TOLLIP) and E-selectin (SELE) in all Cd and MPs treatment groups may indicate the key immune response genes of sea cucumber to Cd exposure and MPs exposure. These genes were involved in the immune defense of sea cucumber exposed to different levels of Cd and MPs. This study provided insights into the mechanism of dietary MPs and Cd intake in an economically and ecologically important invertebrate species.
Does pronator quadratus repair affect functional outcome following volar plate fixation of distal radius fractures? A systematic review and meta-analysis
Authors: Ying Liwei , Cai Guoping , Zhu Zhong , Yu Guoliang , Su Yongwei , Luo Hua 
Published: February 2023

IntroductionThe study aimed to evaluate the efficacy of pronator quadratus (PQ) repair versus no repair following volar plate fixation of distal radius fractures.MethodsA comprehensive search was performed in PubMed, CNKI, EMBASE, Web of Science, Ovid, and Cochrane Library databases. All randomized controlled trials comparing PQ repair with no repair in distal radius fractures before January 2023 were included. Two investigators independently screened eligible articles, assessed the study quality, and extracted data from included studies. Continuous variables used standardized mean difference and 95% confidence interval as efficacy statistics. The meta-analysis was performed using the Revman 5.4 software.ResultsA total of 430 patients in 7 RCT studies were included in this meta-analysis, of which 218 underwent PQ repair, while 212 patients underwent no repair. The results of the meta-analysis displayed statistically significant differences in grip strength (short-term), pronation angle (short-term), and pronation strength (short- and long-term) between the two groups. No significant difference in other outcomes was found between the two treatment arms.DiscussionThe repair of PQ may further increase grip strength and pronation function in the short-term and enhance long-term pronator muscle strength compared to no repair. However, due to the small number of articles included in the study, the above conclusions need to be verified by a larger sample and multi-center clinical study.
Staged stenting strategy of acutely wide-neck ruptured intracranial aneurysms: A meta-analysis and systematic review
Authors: Wei Yanpeng , Zhang Xiaoxi , Zhang Renkun , Zhang Guanghao , Shang Chenghao , Chen Rundong , Li Dan , Huyan Meihua , Wu Congyan , Zong Kang , Feng Zhengzhe , Dai Dongwei , Li Qiang , Huang Qinghai , Xu Yi , Yang Pengfei , Zhao Rui , Zuo Qiao , Liu Jianmin 
Published: February 2023

ObjectiveIn the study, we explored the safety and effectiveness of staged stenting strategy for acutely wide-neck ruptured intracranial aneurysms.MethodsOnline databases, including PubMed, EMBASE, the Cochrane database, and Web of Science, were retrospectively and systematically searched. The main observation indicators were the procedure-related complication rate, complete occlusion rate, and favorable clinical outcome. Meta-analysis was performed using a random or fixed effect model based on heterogeneity.ResultsA total of 5 studies with 143 patients were included. The hemorrhagic complication rate of the initial coiling and staged stenting was 2.8% (4 of 143) and 0, respectively. The ischemic complication rate of the coiling and supplemental stenting was 3.5% (5 of 143) and 2.9% (4 of 139), respectively. There were no deaths due to procedure-related complications in two stages. The aneurysm complete occlusion rate was 25% (95% CI, 0.13–0.03; I2 = 4.4%; P = 0.168) after initial coiling, 54% (95% CI, 0.63–0.64; I2 = 0%; P = 0.872) after staged stenting, and 74% (95% CI, 0.66–0.81; I2 = 56.4%; P = 0.562) at follow-up, respectively. Favorable clinical outcome rate 74% (95% CI, 0.61–0.86; I2 = 50.5%; P = 0.133) after discharge of initial coiling treatment, and 86% (95% CI, 0.80–0.92; I2 = 0; P = 0.410) after discharge from stenting, and 97% (95% CI, 0.93–1.01; I2 = 43.8%; P = 0.130) at follow-up.ConclusionStaged stenting treatment of wide-neck RIA with coiling in the acute phase followed by delayed regular stent or flow-diverter stent had high aneurysm occlusion rate, favorable clinical outcome rate and low procedure-related complication rate. A more dedicated and well-designed controlled study is warranted for further evaluation of staged stenting treatment compared to SCA in wide-neck RIA.
Analyzing and predicting the risk of death in stroke patients using machine learning
Authors: Zhu Enzhao , Chen Zhihao , Ai Pu , Wang Jiayi , Zhu Min , Xu Ziqin , Liu Jun , Ai Zisheng 
Published: February 2023

BackgroundStroke is an acute disorder and dysfunction of the focal neurological system that has long been recognized as one of the leading causes of death and severe disability in most regions globally. This study aimed to supplement and exploit multiple comorbidities, laboratory tests and demographic factors to more accurately predict death related to stroke, and furthermore, to make inferences about the heterogeneity of treatment in stroke patients to guide better treatment planning.MethodsWe extracted data from the Medical Information Mart from the Intensive Care (MIMIC)-IV database. We compared the distribution of the demographic factors between the control and death groups. Subsequently, we also developed machine learning (ML) models to predict mortality among stroke patients. Furthermore, we used meta-learner to recognize the heterogeneity effects of warfarin and human albumin. We comprehensively evaluated and interpreted these models using Shapley Additive Explanation (SHAP) analysis.ResultsWe included 7,483 patients with MIMIC-IV in this study. Of these, 1,414 (18.9%) patients died during hospitalization or 30 days after discharge. We found that the distributions of age, marital status, insurance type, and BMI differed between the two groups. Our machine learning model achieved the highest level of accuracy to date in predicting mortality in stroke patients. We also observed that patients who were consistent with the model determination had significantly better survival outcomes than the inconsistent population and were better than the overall treatment group.ConclusionWe used several highly interpretive machine learning models to predict stroke prognosis with the highest accuracy to date and to identify heterogeneous treatment effects of warfarin and human albumin in stroke patients. Our interpretation of the model yielded a number of findings that are consistent with clinical knowledge and warrant further study and verification.