bluuebunny/crossref_metadata_2025_split huggingface dataset:
Successful ex vivo expansion of tumor infiltrating lymphocytes with systemic chemotherapy prior to surgical resection
Authors: Balzeau Julien , Ravindran Anindita , Wang Xin , Maisuria Jinal , Lucchesi Anna , Yao Hui , Matsueda Satoko 
Published: May 2023

Abstract
Tumor Infiltrating Lymphocytes (TIL) have demonstrated efficacious clinical outcomes for many patients with various types of solid cancers, including melanoma, gastrointestinal cancer, lung cancer, and head & neck cancer. Currently, majority of clinical trials require that patients did not receive systemic therapy right before tumor tissue resection to avoid the interference of chemotherapy in the ex vivo TIL expansion. The primary disadvantage of this strategy is limiting the accessibility of TIL therapy for many eligible cancer patients. Over the past decade, substantial progress has been made for ex vivo expansion technologies in T cells. In this study, we investigated the possibility of enrolling patients who underwent chemotherapy prior to surgical resection. We collected seventeen tumor tissues from treatment naive cases, and five from cases that underwent chemotherapies. Cancer indications enrolled in this study were colorectal, lung, and brain cancers from both primary and metastasis cancers. TILs from these tumors were expanded ex vivo to 2.1E8 on average, with an overall success rate of 90.9%. Subsequently, TIL phenotypes and cytokine production were analyzed using flow cytometry and ELISA, respectively. We demonstrated functional TIL expansion from tumor tissues despite chemotherapy prior to surgical resection. We observed no significant phenotypic or functional differences nor changes between groups with and without chemotherapy. TIL expansion rate and characteristics were similar regardless of chemotherapy prior to resection, thereby providing a possibility to recruit patients with the most recent chemotherapy history in TIL therapy trials.
Immunogenic Cell Death-related Signature Evaluates the Tumor Microenvironment and Predicts the Prognosis in Diffuse Large B-Cell Lymphoma
Authors: Huang# Shengqiang , Liu Wenbin , Zhao Qiuling , Chen Ting , Huang Ruyi , Dong Liangliang , Nian Zilin , Yang Lin 
Published: May 2023

Abstract
Aim
Current literatures suggest a growing body of evidence highlighting the pivotal role of immunogenic cell death (ICD) in multiple tumor types. To address the gap of the potential and mechanisms of ICD in Diffuse Large B cell lymphoma (DLBCL), our current study aims to examine the impact of ICD on DLBCL and identify a corresponding gene signature in DLBC.
Methods
Using the expression profiles of ICD-associated genes, the Gene Expression Omnibus (GEO) samples were segregated into ICD high and low subtypes utilizing non-negative matrix factorization clustering. Next, Univariate and LASSO Cox regression analyses were employed to establish the ICD-related gene signature. Subsequently, the CIBERSORT tool, ssGSEA, and ESTIMATE algorithm were utilized to examine the association between the signature and tumor immune microenvironment of DLBC. Finally, the oncoPredict algorithm was implemented to evaluate drug sensitivity prediction of DLBCL patients.
Results
The findings suggest that the immune microenvironment of the ICD high group with a poor prognosis was significantly suppressed. An 8-gene ICD-related signature was identified and validated to prognosticate and evaluate the tumor immune microenvironment in DLBCL. Similarly, the high-risk group exhibited a worse prognosis compared to the low-risk group, and the immune function was considerably suppressed. Moreover, the results of OncoPredict algorithm indicated that patients in the high-risk group exhibited higher sensitivity to Cisplatin, Cytarabine, Epirubicin, Oxaliplatin, and Vincristine with low IC50.
Conclusion
The present study provides novel insights into the role of ICD in DLBCL by identifying a new biomarker for the disease and may have implications for the development of immune-targeted therapies for the tumor.
Groundwater Quality assessment for Drinking Purpose using Traditional and Fuzzy-GIS based Water Quality Index in Gurugram District of Haryana, India
Authors: Tiwari Akash , Kumar Manish , Thakur Swati 
Published: May 2023

Abstract
Primarily groundwater is consumed for the drinking and irrigation purpose. However, due to increasing anthropogenic activities, its quality and quantity have substantially declined over time. The focus of this study is to evaluate the pre-monsoonal groundwater quality and its spatial variability for drinking purposes in the Gurugram, Haryana, India. Ground Water Quality Index (GWQI) developed on the basis of the Geographical Information System is effective in the assessment of groundwater quality and its spatial variability, but it is unable to account for uncertainties related to environmental problems. Thus, a Hybrid Fuzzy-GIS based Water Quality Index (FGQI) has been proposed for the assessment of groundwater quality. The study conducted factor analysis to identify the prime factors responsible for groundwater contamination and collected pre-monsoonal groundwater samples through primary sampling. The groundwater quality was assessed based on eight hydro geochemical parameters (pH, TDS, Calcium, Chloride, Sulphate, Fluoride, Potassium, and Sodium). The spatial interpolation of each parameter was performed using appropriate techniques, selected based on a normality test. The guidelines of the World Health Organization (WHO) and Bureau of Indian Standard (BIS) were incorporated in the development of GWQI and FGQI, respectively. Correlation analysis was performed to determine the best fuzzy overlay technique for FGQI, and the Fuzzy GAMMA technique with gamma equal to 0.9 was selected. Finally, the GWQI and FGQI were classified into three classes: unsuitable, moderate suitable, and suitable using "natural break." A higher index indicates a higher water quality. The results show that the groundwater in the central part of Gurugram is suitable for drinking, while it is not suitable in the extreme north, south-east, and western regions. In conclusion, this study finds that FGQI effectively assesses the groundwater quality in the region better than GWQI.
Higher Education for Sustainable Construction: Assessing Civil Engineering and Architecture Curricula at Nine Burundian Universities
Authors: Rudahinyuka Samuel , Ngenzi Jean Claude , HongBing Li , Kubwarugira Gamaliel , Hongjie Xie , Ndihokubwayo Athanase 
Published: May 2023

Abstract
Civil engineering is changing and needs to change more especially since it plays a main role as a fundamental activity in the creation of services and infrastructures with a high environmental impact. For many years, the need arose to equip civil engineering students, engineers, and architects of tomorrow with a wider horizon of environmental, economic, and social concepts for decision-making sensitive to sustainability issues. Nevertheless, a vast literature on the evaluation of higher education institutions’ involvement in including sustainability concept in the curricula exists in abundance; but an absence of studies on the inclusion of this concept in curricula by specific university faculties such as civil engineering is readily apparent. Therefore, this study aims to evaluate the contribution of the civil engineering curriculum in Nine Burundian Universities to integrating sustainability in the civil engineering industry in Burundi and abroad. The evaluation of program models following the Sustainability Tracking, Assessment, and Rating System shows a weak Burundian universities’ commitment to integrating sustainability into the civil engineering curricula and sustainability concepts in the civil engineering industry. For better participation of higher education institutions involved in the training of civil engineers and architects in Burundi to promote sustainable construction technologies adoption; a restructuring of the teaching programs in the faculty of civil engineering and architecture by adding courses related to sustainable construction and those including some sustainable construction gears is proposed. The lack of studies and publications in this field in the Burundian context limited this study.
Interdisciplinary Perceptions and Supportive Care for Biblical Framework Counseling (BFC) Clients
Authors: Oji Valerie U , Powell Bailey 
Published: May 2023

Abstract
Spirituality is considered important for culturally sensitive, patient-focused care. Still, there may be limitations with spiritual care delivery or guidelines. The study explores interdisciplinary perspectives on supportive care for individuals encountered who choose a biblical framework intervention for mental health treatment. A qualitative literature synthesis was conducted, and an online survey with an open-ended anonymous questionnaire were used to review a case described by a Biblical Framework Counseling (BFC) provider. Various providers identified in the literature utilized religious/spiritual (R/S), medical and/or psychological monotherapy, or integrated interventions. Fifty-four multidisciplinary professionals were surveyed, and responses analyzed. Preliminary themes included willingness to coordinate care for individuals with biblical beliefs, the capacity to which health professionals would provide care, and perceptions of BFC efficacy. R/S care was reportedly integrated as a part of a holistic approach; regular follow-ups or ethical hand-off/referral for BFC clients was considered due to provider comfort level with BFC monotherapy, perceived relapse potential, with ongoing medication management, individual therapy, and spiritual maintenance recommended. The case revealed contrasts in BFC and nonR/S provider perspectives on pertinent mental health history and inferences. Significant client information for support were confirmatory diagnosis, symptoms, comorbidities, relevant childhood issues, faith-health beliefs, family history and genetics, medication and therapy adherence, substance use. Providers’ faith-health belief congruence with BFC clients and ethical decisionmaking should be considered. GME programs may consider these, as well as R/S intervention types and multidisciplinary provider scope of practice as options for clinician training.
Induced Mutagenesis for Enhancing Genetic Variability and Agronomic Performance in Sorghum Varieties for Burkina Faso
Authors: Nikièma Minimassom Philippe 
Published: May 2023

Abstract

Sorghum is one of the main cereals in terms of production and cultivated area in Burkina Faso. However, genetic variability in Sorghum varieties is low. The study aims to induce genetic variability in farmers’ preferred sorghum varieties and select the novel desired traits. Seeds of two Sorghum varieties (Grinkan and Sariaso14) were irradiated with gamma rays at 200, 300, 400 and 500 Gy doses. The mutant populations were developed using the single seed descend method. A 2-years field assessment was conducted to assess the genetic variability among 403 advance generation Sorghum mutants. A significant difference between the doses was observed for leaf number plant− 1 (NPL) (P ≤ 1%) and the plant height (PlHe) (P ≤ 5%) of Sariaso14 lines and only for the PlHe (P ≤ 1%) of Grinkan lines. All irradiation doses significantly increased the grain weight panicle− 1 of Sariaso14 lines while they affected those of Grinkan lines. The genotypic coefficient of variation (GCV), the phenotypic coefficient of variation (PCV), the broad sense heritability (H2) and the genetic gain (GG) were high for grain weight panicle− 1 (GrWe). They were 90.82%, 92.84%, 0.95 and 183%, respectively for Sariaso14 lines and 80.85%, 89%, 0.82 and 151.3% respectively for Grinkan lines demonstrating that the observed variations for this trait was mainly due to genetic factors. The clustering of the mutants showed that the mutagenesis allowed the selection of dwarf, early maturity and high grain weight mutants. Thus, the induced mutation has improved the agronomic performances of sorghum varieties.
Potassium isotope fractionation during silicate-carbonatite melt immiscibility and phlogopite fractional crystallization
Authors: Su Ben-Xun , Pan Qi-Qi , Bai Yang , Li Wen-Jun , Cui Meng-Meng , Pang Kwan-Nang 
Published: May 2023

Abstract
Potassium (K) isotopes have been used as a tracer of K recycling in the Earth, but K isotope fractionation during magma evolution is poorly constrained. Here, we present K isotope data for a magmatic suite of alkaline silicate-carbonatite affinity. The suite was formed from liquid-liquid immiscibility and subsequent phlogopite fractionation. The K isotopic signatures of different rock types are in the following order: alkaline silicate lavas (δ41K = –0.424 to 0.090‰) &gt; carbonatitic silicate lavas (δ41K = –0.640 to –0.035‰) &gt; carbonatites (δ41K = –0.858 and –0.258‰). Phlogopite phenocrysts in the silicate lavas are isotopically lighter (δ41K = –0.628 to –0.534‰) than the lavas in which they occur (Δ41KPhlogopite-whole rock = –0.502 to –0.109‰). Correlations between δ41K values and chemical proxies of melt immiscibility and phlogopite fractionation indicate that K isotopes are significantly fractionated by both processes at a ~0.6‰ magnitude. Such K isotope variation overlaps the range of δ41K in arc lavas. Compilations of literature data further confirm the critical roles of melt immiscibility and phlogopite fractionation in K isotope variations of high-K lavas (K2O &gt;1 wt%) from post-collision orogenic and intra-continental settings. In comparison, basaltic arc lavas are depleted in K2O (mostly &lt;1 wt%) and lack evidence of significant phlogopite fractionation. The K isotope variations of arc lavas are mainly controlled by their mantle sources, which were metasomatized by melt or fluid released from the subducting slab. Therefore, K recycling and K isotope variation are controlled by distinct mechanisms in different tectonic settings.
Pitch stability of offshore waste collection platform hull with NACA fin stabilizers
Authors: Wen Ziqi , Si Mingqing , Wang Chun , Yang Fuqin 
Published: May 2023

In view of the safety problems caused by the pitch stability of the offshore waste collection platform, the three-dimensional model of the collection platform hull was established by SolidWorks software, and the influence of the installation parameters of NACA fin stabilizers on the pitch stability of the collection platform hull was discussed based on three-dimensional potential flow theory. The results show that the installation parameters of NACA fin stabilizers have an essential influence on the pitch stability of the collection platform hull. The larger the positive installation angle and the farther away the bow fin is from the midship, the smaller the peak values of pitch motion response. While the tail fin is not suitable to be installed near 600 mm of midship to avoid severe longitudinal movement of the hull.
Investigation on the flow-induced tangential force characteristic of labyrinth seals under different operation parameters
Authors: Gu Qianlei , Yang Jiangang 
Published: May 2023

The flow-induced tangential force Ft is crucial for the annular gas seal stability. In this paper, a three-dimensional CFD model is established to study the Ft characteristics of the labyrinth seal. The overall Ft of the seal section is decomposed into each seal cavity. The Ft characteristic of each seal cavity versus rotation speed ω and inlet pressure pin is analyzed. The result shows that the sign of Ft in each seal cavity changes from the negative to the positive along the leakage direction, resulting in a specific crossing tooth number Nc at which Ft≈ 0 N. When the tooth number is smaller than Nc, the seal stability increases with the increase of ω and pin. When the tooth number is larger than Nc, the trend is reversed. When the tooth number equals Nc, the seal stability is in a critical state, showing no dependency to the ω and pin. The Nc is a novel index for the labyrinth seal stability.
Effects of raw water quality on the adsorptive removal of 2-methylisoborneol by powdered activated carbon under non-equilibrium conditions
Authors: Asada Yasuhiro , Hayasaka Shunichi , Miyoshi Taro , Tokuyasu Marina , Akiba Michihiro 
Published: May 2023

Abstract

Natural organic matter contained in natural water inhibits the adsorptive removal of 2-methylisoborneol (2-MIB) by powdered activated carbon (PAC). We investigated the relationship between water-quality indices and the adsorptive removal of 2-MIB by PAC. We collected three different raw water (i.e., two lake water and one river water) samples twice per month for 10 months. We characterized the raw water using total organic carbon concentration, ultraviolet absorption at 254 nm, electrical conductivity, and excitation–emission matrix analysis. The results were compared with 2-MIB removal rates evaluated from PAC adsorption experiments and revealed that there was no universal indicator that could explain the trends of the 2-MIB removal rate during the overall experimental period. The correlation trends between 2-MIB removal rates and water-quality indices differed significantly between the high and low water-temperature periods. Several water-quality indices related to the organic matter associated with biological processes, especially algal activities (i.e., soluble microbial products, chlorophylls, and phycocyanin), exhibited significant correlations with the 2-MIB removal rates (|R| &gt; 0.7) under certain conditions (e.g., high lake-water temperature). Both the parallel factor (PARAFAC) analysis and fluorescence regional integration (FRI) method could evaluate such behaviors after including the regions associated with algal organic matter in the calculation.
Process performance evaluation of faecal matter treatment via black soldier fly
Authors: Achieng Oyoo Valary , Riungu Joy Nyawira , Dey Prasanta , Kirimi James Gitonga , Matheka Rosemary M. 
Published: May 2023

Abstract

Sustainable management of faecal matter is a prevailing global challenge. In this study, we assessed black soldier fly (BSF) process performance during co-treatment of faecal matter using kitchen waste (FM:KW) to formulate five feeding substrates. About 1 kg of each feed substrate was treated utilizing 5 g of 5-day-old BSF larvae after which 100 larvae were randomly picked at 3-day intervals from each treatment to monitor the larval weight gain across the treatment process. Larval days to 50% pupation, mean pupal yield, waste reduction rate (WR), bioconversion rates (BRs), and feed conversion rates (FCRs) were monitored for the process performance. Study results showed that the substrate 1:1 attained the best measures of high WR, waste reduction index (WRI), BR, FCR, and overall pre-pupal yield within a shorter development time. Further, we modelled the BSF larval weight gain using the modified Gompertz model to assess the least time for optimal biomass conversion for animal feed processing. The BSF larvae exhibited an S-shaped growth curve and the modified Gompertz model adequately quantified the BSF larval growth performance. In the future, our methodology will pave the way for effective treatment and valorization of faecal matter from onsite sanitation facilities, manage organic municipal wastes and provide alternative animal feed and bio-fertilizer.
Anti-obesity Properties of Phytochemicals: Highlighting their Molecular
Mechanisms against Obesity
Authors: Poulios Efthymios , Koukounari Stergia , Psara Evmorfia , Vasios Georgios K. , Sakarikou Christina , Giaginis Constantinos 
Published: May 2023

Abstract:
Obesity is a complex, chronic and inflammatory disease that affects more than
one-third of the world’s population, leading to a higher incidence of diabetes, dyslipidemia,
metabolic syndrome, cardiovascular diseases, and some types of cancer. Several
phytochemicals are used as flavoring and aromatic compounds, also exerting many benefits
for public health. This study aims to summarize and scrutinize the beneficial effects
of the most important phytochemicals against obesity. Systematic research of the current
international literature was carried out in the most accurate scientific databases, e.g.,
Pubmed, Scopus, Web of Science and Google Scholar, using a set of critical and representative
keywords, such as phytochemicals, obesity, metabolism, metabolic syndrome,
etc. Several studies unraveled the potential positive effects of phytochemicals such as berberine,
carvacrol, curcumin, quercetin, resveratrol, thymol, etc., against obesity and
metabolic disorders. Mechanisms of action include inhibition of adipocyte differentiation,
browning of the white adipose tissue, inhibition of enzymes such as lipase and amylase,
suppression of inflammation, improvement of the gut microbiota, and downregulation
of obesity-inducing genes. In conclusion, multiple bioactive compounds-phytochemicals
exert many beneficial effects against obesity. Future molecular and clinical studies
must be performed to unravel the multiple molecular mechanisms and anti-obesity activities
of these naturally occurring bioactive compounds.
LncRNA GAS5 as an Inflammatory Regulator Acting through Pathway in
Human Lupus
Authors: Xiao Jianping , Wang Deguang 
Published: May 2023

Aims:
To investigate the contribution of GAS5 in the pathogenesis of SLE.

Background:
Systemic Lupus Erythematosus (SLE) is characterized by aberrant activity of the immune system,
leading to variable clinical symptoms. The etiology of SLE is multifactor, and growing evidence has
shown that long noncoding RNAs (lncRNAs) are related to human SLE. Recently, lncRNA growth arrest-specific
transcript 5 (GAS5) has been reported to be associated with SLE. However, the mechanism between
GAS5 and SLE is still unknown.

Objective:
Find the specific mechanism of action of lncRNA GAS5 in SLE.

Methods:
Collecting samples of the SLE patients, Cell culture and treatment, Plasmid construction, and transfection,
Quantitative real-time PCR analysis, Enzyme-linked immunosorbent assay (ELISA), Cell viability analysis,
Cell apoptosis analysis, Western blot.

Results:
In this research, we investigated the contribution of GAS5 in the pathogenesis of SLE. We confirmed
that, compared to healthy people, the expression of GAS5 was significantly decreased in peripheral monocytes
of SLE patients. Subsequently, we found that GAS5 can inhibit the proliferation and promote the apoptosis of
monocytes by over-expressing or knocking down the expression of GAS5. Additionally, the expression of
GAS5 was suppressed by LPS. Silencing GAS5 significantly increased the expression of a group of
chemokines and cytokines, including IL-1β, IL-6, and THFα, which were induced by LPS. Furthermore, it was
identified the involvement of GAS5 in the TLR4-mediated inflammatory process was through affecting the activation
of the MAPK signaling pathway.

Conclusion:
In general, the decreased GAS5 expression may be a potential contributor to the elevated production
of a great number of cytokines and chemokines in SLE patients. And our research suggests that GAS5 contributes
a regulatory role in the pathogenesis of SLE, and may provide a potential target for therapeutic intervention.