bluuebunny/crossref_metadata_2025_split huggingface dataset:
Protective effects of esculetin against ovary ischemia–reperfusion injury model in rats
Authors: Güvenç Mehmet , Yüksel Murat , Kutlu Tuncer , Etyemez Muhammed , Gökçek İshak , Cellat Mustafa 
Published: September 2023

AbstractAimsOvarian ischemia–reperfusion (I/R) injury is a phenomenon that necessitates urgent intervention, which occurs as a result of ovarian torsion, and it is frequently seen in young women. A large amount of free radical and oxidative damage as a result of I/R plays a role in the cause of the incident. Antioxidant agents are thought to be beneficial in preventing this damage, and the potential protective effects of esculetin, which had not been tested previously, were investigated in this study.Study DesignThe rats in the study were divided into five groups at random: control, sham, esculetin, I/R, and treatment. Oxidative stress parameters, proinflammatory cytokines, nuclear factor erythroid 2‐related factor 2 (Nrf‐2)/nuclear factor‐kβ (NF‐κβ) pathway, and histopathological analyses were evaluated at the end of the study.Key FindingsAfter I/R, malondialdehyde levels, proinflammatory cytokines, tumor necrosis factor‐α and interleukin‐1β levels and NF‐κβ expressions were increased, Nrf‐2 expression and glutathione level decreased and the histopathologic picture deteriorated. However, as a result of the esculetin treatment, ameliorative effects in the aforementioned parameters were determined, and it was ensured that they returned to normal levels.ConclusionAccording to these findings, esculetin has protective effects on I/R damage by lowering lipid peroxidation and having antioxidant and anti‐inflammatory properties.SignificanceOur results proved the protective effect of esculetin against ovarian IR injury in rats and this may be attributed to Nrf‐2/NF‐κβ axis which showed antioxidant and anti‐inflammatory effects. Therefore, esculetin can be used in the future for preventive effects to ovarian IR injury.
Development of lectin‐based lateral flow assay for fucosylated alpha‐fetoprotein
Authors: Puangpila Chanida , Anukulkich Nattakritta , Chiapleam Siraphatsorn , Intajan Benyarat , Jakmunee Jaroon , Pencharee Somkid 
Published: September 2023

AbstractFucosylated alpha‐fetoprotein (AFP‐L3) is a more specific and sensitive biomarker for early diagnosis of hepatocellular carcinoma (HCC) than only the alpha‐fetoprotein (AFP) level. Rapid and simple detection of AFP‐L3 level greatly facilitates the early detection as well as the treatment of HCC, resulting in the reduction of mortality. Here, we developed a rapid and sensitive lateral flow assay (LFA) using lectin Lens culinaris agglutinin (LCA), which has a specific affinity to AFP‐L3 fraction of AFP, as a biorecognition element for determination of the fucosylation of AFP. The assay is based on a sandwich format performed on a lateral flow test strip. LCA was immobilized on the membrane as a test line (T). Quantitative detection of AFP‐L3 was achieved by measuring the green color intensity of captured gold nanoparticle conjugates on the T and control line (C) utilizing an in‐house test strip reader. The calculated absorbance obtained by the green color intensity signals proportionally increased with AFP concentrations. The developed lectin‐based LFA provided a detection limit of 0.8 ng/mL for AFP with a linear range between 1.5 and 160.0 ng/mL within an assay time of 10 min. Recoveries between 74.5% and 113.2% with relative standard deviations of 5.2%–8.7% for measuring spiked human serum were also achieved. The results reveal that the proposed assay offers a rapid, sensitive, and specific method, which is useful for development in point‐of‐care testing for early detection and treatment of HCC.
Characterization of pediatric transfusion‐dependent thalassemia patients in a large academic center
Authors: Zarrabian Dorsa , Hanna Mirette 
Published: September 2023

AbstractBackgroundTransfusion‐dependent thalassemia patients are at high risk of transfusion‐related complications. Yet, there is scanty data on the frequency of transfusion reactions, particularity alloimmunization among pediatric transfusion‐dependent thalassemia patients. In addition, there is no consensus on the prophylactic antigen matching for prevention of alloimmunization or the extent of antigen matching for alloimmunized thalassemia patients.MethodsWe conducted a retrospective study to assess the frequency and specificity of alloimmunization among pediatric transfusion‐dependent thalassemia patients receiving ABO, RhD, and K‐matched red blood cell units. In addition, we studied the association between patients' characteristics and alloimmunization. The clinical and transfusion records of transfusion‐dependent thalassemia patients followed up at our institution between July 2018 and June 2022 were reviewed.ResultsNinety‐two transfusion‐dependent thalassemia patients having mean age of 13.37 years (SD, 5.56) were included in our study. Eight patients (9%) had developed clinically significant alloantibodies; six patients (6%) developed alloantibody against E antigen while two patients (2%) developed more than one alloantibody. Of alloimmunized patients, five patients had received transfusion outside Canada. Patients' sex, age, having a genotype variant, total number, and duration of transfusion received were not associated with the risk of alloimmunization. The transfusion‐recipient's diagnosis of β‐thalassemia, having developed autoantibody, and history of receiving transfusion outside Canada were associated with alloimmunization.ConclusionBlood matching for ABO, RhD, and K antigens resulted in, although not eliminated, lower frequency of alloimmunization than that previously reported among pediatric thalassemia patients. Extending matching to include Rh antigens could further reduce the rate of alloimmunization.
Simulating fate and transport of non‐steroidal anti‐inflammatory drugs using Root Zone Water Quality Model 2
Authors: Shu Weixi , Price Gordon W. , Jamieson Rob , Lake Craig 
Published: September 2023

AbstractThe fate and transport of non‐steroidal anti‐inflammatory drugs (NSAIDs) in soil are determined by various processes, and the complexity of the system lends itself to the use of computer simulation models to help understand it. This study demonstrated the first attempt to use empirical data from lab incubation and field studies to parameterize and test a process‐based agricultural systems model, Root Zone Water Quality Model 2 (RZWQM2), to simulate the fate and transport of naproxen (NPX), ibuprofen (IBF), and ketoprofen (KTF) in field‐based lysimeters amended with alkaline‐treated biosolids (ATBs). The model calibrated for the soil–water balance module and contaminant transport module was used to predict water seepage through the soil profile in 2017 and 2018 within a 15% error of the field measured data, with model performance statistics such as Nash—Sutcliffe model efficiency (NSE) and R2 all greater than 0.70. The overall predicted percent recovery of initial spiked NSAIDs in both soil and water samples, after further calibration of the contaminant transport module, was within the same order of magnitude as the measured data. The model underestimated the percent recovery of initial spiked NSAIDs at the 30‐ to 55‐cm soil depth for all treatments on day 3. The calibrated soil subsurface aerobic half‐lives of NPX and IBF were found to be considerably lower than their laboratory‐measured half‐lives obtained from the incubation study. The overall performance of RZWQM2 in simulating the soil hydrology and behavior of NSAIDs in soil profiles receiving various rates of ATB amendments was satisfactory.
Exploring spiritual/religious coping strategies among mothers of children with severe or profound intellectual disability during genetic counseling in Brazil
Authors: Rodrigues Stephania de Araújo , de Carvalho Ellen Zapparoli Nunes , Campos Crispim Antonio , Fontanella Bruno José Barcellos , Melo Débora Gusmão 
Published: September 2023

AbstractParents use several coping strategies to deal with the challenges of caring for children with disabilities. This qualitative study explored the spiritual/religious coping strategies adopted by Brazilian mothers of children with severe or profound intellectual disabilities who were attending the medical genetics clinic for genetic counseling. Individual semi‐structured interviews were conducted with 15 participants, selected by convenience. Thematic content analysis was used to investigate the data. Five major themes were recognized: (1) spirituality/religion as a source of resilience; (2) surrender to God's plan; (3) a feeling of predestination; (4) children with a disability perceived/conceived as a blessing; and (5) pleading for divine intervention. Participants highlighted the ability to recover and adapt to bad circumstances and stressful events with God's support using spiritual/religious resources and practices. They attributed the cause and control of events in their lives to an external and transcendental source, showing themselves to be resigned to the condition of their child with a disability and his/her demands. The feeling of predestination appeared as a form of divine intervention as well as a way of attributing meaning to the situation. Their child with a disability was compared constantly to an “angel,” a celestial being created by God to be a messenger and fulfill divine purposes. From the participants' perspective, these coping mechanisms appeared to have helped them adjust to the situation. Spirituality and religiousness have become crucial in cultures such as Brazil, where religious practices, often highly syncretic, are common. A more detailed and comprehensive understanding of how spiritual/religious coping strategies are mobilized during the genetic counseling process can improve the cultural competence of genetic counselors and help guide proposals for more effective clinical interventions.
Structural elucidation of amino amide‐type local anesthetic drugs and their main metabolites in urine by LC‐MS after derivatization and its application for differentiation between positional isomers of prilocaine
Authors: Weissberg Avi , Drug Eyal , Prihed Hagit , Madmon Moran , Shamai Yamin Tamar 
Published: September 2023

AbstractThe demand for clinical toxicology analytical methods for identifying drugs of abuse and medicinal drugs is steadily increasing. Structural elucidation of amino amide‐type local anesthetic drugs and their main metabolites by GC‐EI‐MS and LC‐ESI‐MS/MS is of great analytical challenge. These compounds exhibit only/mostly fragments/product ions representing the amine‐containing residue, while the aromatic amide moiety remains unidentified. This task becomes even more complicated when discrimination between positional isomers of such compounds is required. Here, we report the development of a derivatization procedure for the differentiation and structural elucidation of a mixture of local anesthetic drugs and their metabolites that possess tertiary and secondary amines in water and urine. A method based on two sequential “in‐vial” instantaneous derivatization processes at ambient temperature followed by LC‐ESI‐MS/MS analysis was developed. 2,2,2‐Trichloro‐1,1‐dimethylethyl chloroformate (TCDMECF) was utilized to selectively convert the secondary amines into their carbamate derivatives, followed by hydrogen peroxide addition to produce the corresponding tertiary amine oxides. The resulting derivatives exhibited rich fragmentation patterns, enabling improved structural elucidation of the original compounds. The developed method was successfully applied to the differentiation and structural elucidation of prilocaine and its four positional isomers, which all possess similar GC and LC retention times and four of them exhibit almost identical EI‐MS and ESI‐MS/MS spectra, enabling their structural elucidation in a single LC‐ESI‐MS/MS analysis. The developed technique is fast and simple and enables discrimination between isomers based on different diagnostic ions/fragmentation patterns.
YAP suppresses human T‐cell leukemia virus type 1 transcription
Authors: Li Hengbo , Zou Feng , Zhang Jie , Zhu Shengyu , Chu Kaifei , Zhang Xu , Zhao Tiejun 
Published: September 2023

AbstractHuman T‐cell leukemia virus type 1 (HTLV‐1) is an oncogenic retrovirus that causes adult T‐cell leukemia/lymphoma (ATL). HTLV‐1 encodes Tax protein that activates transcription from viral long terminal repeats (LTR). Multiple cofactors are involved in the regulation of HTLV‐1 transcription via association with Tax. Yes‐associated protein (YAP), which is the key effector of Hippo pathway, is elevated and activated in ATL cells. In this study, we reported that YAP protein suppressed Tax activation of HTLV‐1 5′ LTR but not 3′ LTR. The activation of the 5′ LTR by Tax was potentiated when YAP was depleted. Moreover, overexpression of YAP repressed HTLV‐1 plus‐strand viral gene expression and virion production, whereas compromising YAP by RNA inference augmented the expression of HTLV‐1 protein. As mechanisms of YAP‐mediated viral transcription inhibition, we found that YAP interacted with Tax, and prevented the association between Tax and p300. It finally led to the inhibition of recruitment of Tax to the Tax‐responsive element in the 5′ LTR of HTLV‐1. Taken together, our results demonstrate the negative regulatory function of YAP in Tax activation of HTLV‐1 transcription. It may achieve sufficient transcriptional repression to maintain persistent infection and long‐term latency of HTLV‐1 in the host cells.
Intravenous tecovirimat treatment of oral monkeypox lesions in a patient with HIV‐1
Authors: Chen Wan Hsiang , Axell‐House Dierdre B. 
Published: September 2023

AbstractA man in his mid‐30s presented with 1 week of painful, progressive, and confluent tongue and chin ulcerations. Swabs of the lesions were detected positive for monkeypox (mpox) DNA by polymerase chain reaction. During hospitalization, he received a diagnosis of human immunodeficiency virus‐1. Due to severe oral pain, the patient was treated with intravenous tecovirimat 200 mg every 12 h for 4 days with remarkable improvement in lingual lesions. He completed a total 14‐day course with oral tecovirimat. Our patient's clinical progress contributes to the data available for the potential efficacy of intravenous tecovirimat for mpox infection in humans.
Designing the transition to operations in large inter‐organizational projects: Strategy, structure, process, and people
Authors: Zhang Xinyue , Denicol Juliano , Chan Paul W. , Le Yun 
Published: September 2023

AbstractThe transition from projects to operations requires a spanning from more temporary, goal‐oriented, and evolving organizational forms to more permanent, routine, and ongoing organizational forms. A question of practical and theoretical significance is how to organize the transition to operations in large inter‐organizational projects. To answer this question, we conducted a longitudinal case study of Beijing Daxing International Airport, which is the largest transportation hub in China to date, and provides rich evidence for successfully managing the transition to operations. By analyzing the organizational design strategy, structures, processes, and management of people in the transition, we provide a synthetic framework for designing the transition to operations in large inter‐organizational projects. The framework provides a set of considerations to design organizational boundaries that build connections, emphasize coordination, and achieve continuity between projects and operations. This study contributes to the nexus of operations management and project management and the organizational design of large inter‐organizational projects.
Impact of surface characteristics on the peri‐implant microbiome in health and disease
Authors: Sinjab Khaled , Sawant Shriya , Ou Alice , Fenno J. Christopher , Wang Hom‐Lay , Kumar Purnima 
Published: September 2023

AbstractBackgroundBecause little is known about the impact of implant surface modifications on the peri‐implant microbiome, we aimed to examine peri‐implant communities in various surface types in order to better understand the impact of these surfaces on the development of peri‐implantitis (PI).MethodsOne hundred and six systemically healthy individuals with anodized (AN), hydroxyapatite‐coated (HA), or sandblasted acid‐etched (SLA) implants that were >6 months in function were recruited and categorized into health (H) or PI. Peri‐implant biofilm was analyzed using 16S rRNA gene sequencing and compared between health/disease and HA/SLA/AN using community‐level and taxa‐level metrics.ResultsHealthy implants did not demonstrate significant differences in clustering, alpha‐ or beta‐diversity based on surface modification. AN and HA surfaces displayed significant differences between health and PI (p < 0.05); however, such a clustering was not evident with SLA (p > 0.05). AN and HA surfaces also differed in the magnitude and diversity of differences between health and PI. Six species belonging to the genera Shuttleworthia, Scardovia, and Prevotella demonstrated lower abundances in AN implants with PI, and 18 species belonging to the genera Fretibacterium, Tannerella, Treponema, and Fusobacterium were elevated, while in HA implants with PI, 20 species belonging to the genera Streptococcus, Lactobacillus, Veillonella, Rothia, and family Ruminococcaceae were depleted and Peptostreptococcaceae, Atopobiaceae, Veillonellaceae, Porphyromonadaceae, Desulfobulbaceae, and order Synergistales were enriched.ConclusionsWithin the limitations of this study, we demonstrate that implant surface can differentially modify the disease‐associated microbiome, suggesting that surface topography must be considered in the multi‐factorial etiology of peri‐implant diseases.
Problematic alcohol use in young adults exposed to childhood trauma: The Trøndelag Health (HUNT) Study
Authors: Stangeland Helle , Aakvaag Helene Flood , Baumann‐Larsen Monica , Wentzel‐Larsen Tore , Storheim Kjersti , Zwart John Anker , Dyb Grete , Stensland Synne Øien 
Published: September 2023

AbstractProblematic alcohol use (PAU) severely impacts the health, functioning, and long‐term prospects of young people. Prior research indicates that childhood trauma exposure may be an important risk factor for PAU, but few longitudinal studies have looked at how specific trauma types influence this risk. The aim of this study was to investigate the association between childhood trauma exposure and PAU in a large, population‐based cohort of young people. The study sample included 1,913 adolescents who participated in the Trøndelag Health Study (HUNT) between 2006 and 2008 (age range: 12–20) and completed follow‐up 10 years later as young adults (age range: 22–32). The results revealed an increased risk of PAU in young adults exposed to childhood trauma, especially direct physical violence, OR = 2.38, [95% CI 1.56, 3.64]. Young adults who had witnessed violence, OR = 1.55, [95% CI 1.11, 2.17], or experienced an accident, disaster, or other traumatic event, OR = 1.60, [95% CI 1.19, 2.15], also had higher odds of PAU compared to those without such experiences. These associations remained consistent after adjusting for symptoms of headaches and pain as well as posttraumatic and general psychological distress as reported by the participants in adolescence. Future prevention efforts targeting PAU among adolescents and young adults should address violence and other trauma exposure as potential drivers of problematic drinking.
Music Perception in Bone‐Anchored Hearing Implant Users
Authors: Jiam Nicole T. , Formeister Eric J. , Chari Divya A. , David Abel P. , Alsoudi Amer F. , Purnell Stephanie , Jiradejvong Patpong , Limb Charles J. 
Published: September 2023

ObjectiveMusic is a highly complex acoustic stimulus in both spectral and temporal contents. Accurate representation and delivery of high‐fidelity information are essential for music perception. However, it is unclear how well bone‐anchored hearing implants (BAHIs) transmit music. The study objective is to establish music perception performance baselines for BAHI users and normal hearing (NH) listeners and compare outcomes between the cohorts.MethodsA case‐controlled, cross‐sectional study was conducted among 18 BAHI users and 11 NH controls. Music perception was assessed via performance on seven major musical element tasks: pitch discrimination, melodic contour identification, rhythmic clocking, basic tempo discrimination, timbre identification, polyphonic pitch detection, and harmonic chord discrimination.ResultsBAHI users performed comparably well on all music perception tasks with their device compared with the unilateral condition with their better‐hearing ear. BAHI performance was not statistically significantly different from NH listeners' performance. BAHI users performed just as well, if not better than NH listeners when using their control contralateral ear; there was no significant difference between the two groups except for the rhythmic timing (BAHI non‐implanted ear 69% [95% CI: 62%–75%], NH 56% [95% CI: 49%–63%], p = 0.02), and basic tempo tasks (BAHI non‐implanted ear 80% [95% CI: 65%–95%]; NH 75% [95% CI: 68%–82%, p = 0.03]).ConclusionsThis study represents the first comprehensive study of basic music perception performance in BAHI users. Our results demonstrate that BAHI users perform as well with their implanted ear as with their contralateral better‐hearing ear and NH controls in the major elements of music perception.Level of Evidence3 Laryngoscope, 134:1381–1387, 2024
Modified individualized titanium mesh in orbital floor reconstruction for preventing exposure
Authors: Yu Yao , Zhang Wen‐Bo , Sun Qian , Peng Xin 
Published: September 2023

AbstractObjectiveTo describe a novel method of medial fixation of titanium mesh with a right‐angled screwdriver for orbital floor and maxillary reconstruction and to compare the reconstruction outcome of orbital floor reconstruction with modified and traditional methods.MethodsThe data of 23 patients who underwent maxillectomy and orbital floor defect reconstruction by individualized titanium mesh in Peking University School and Hospital of Stomatology between 2018 and 2021 were retrospectively reviewed. While eight patients received modified orbital floor reconstruction with titanium mesh and angled screwdriver (group A), 15 patients received traditional orbital floor reconstruction (group B). The contact area with buccal flap for titanium mesh in groups A and B was calculated. Titanium mesh deformation, fracture or exposure was recorded. Postoperative ophthalmic function and success of esthetic restoration were assessed.ResultsMean follow‐up was for 15.7 months (range, 9–22 months). The contact area with buccal flap for the modified titanium mesh in group A (13.11 ± 1.41 cm2) was significantly less than that of the traditional titanium mesh in group B (21.83 ± 1.23 cm2; p < .05). The exposure of titanium mesh occurred in two patients in group B. The self‐evaluation of facial symmetry for 23 patients showed no significant difference between group A (7.75 ± 0.71) and group B (6.68 ± 1.30; p > .05). No specific complications were reported.ConclusionWe propose a novel method of zygomatic medial fixation of titanium mesh with a right‐angled screwdriver for orbital floor and maxillary reconstruction, which has the potential to prevent the postoperative exposure of titanium mesh.Level of EvidenceLevel III (Retrospective comparative study).