bluuebunny/crossref_metadata_2025_split huggingface dataset:
PKC-ζ mediated reduction of the extracellular vesicles-associated TGF-β1 overcomes radiotherapy resistance in breast cancer
Authors: Zhang Fayun , Zheng Zifeng , Wang Luoyang , Zeng Wenfeng , Wei Wenjing , Zhang Chunling , Zhao Ziran , Liang Wei 
Published: April 2023

Abstract
Background
Radiotherapy is widely applied in breast cancer treatment, while radiotherapy resistance is inevitable. TGF-β1 has been considered to be an endogenous factor for the development of radiotherapy resistance. As a large portion of TGF-β1 is secreted in an extracellular vesicles-associated form (TGF-β1EV), particularly in radiated tumors. Thus, the understanding of the regulation mechanisms and the immunosuppressive functions of TGF-β1EV will pave a way for overcoming the radiotherapy resistance in cancer treatment.

Methods
The superoxide-Zinc-PKC-ζ-TGF-β1EV pathway in breast cancer cells was identified through sequence alignments of different PKC isoforms, speculation and experimental confirmation. A series of functional and molecular studies were performed by quantitative real-time PCR, western blot and flow cytometry analysis. Mice survival and tumor growth were recorded. Student’s t test or two-way ANOVA with correction was used for comparisons of groups.

Results
The radiotherapy resulted in an increased expression of the intratumoral TGF-β1 and an enhanced infiltration of the Tregs in the breast cancer tissues. The intratumoral TGF-β1 was found mainly in the extracellular vesicles associated form both in the murine breast cancer model and in the human lung cancer tissues. Furthermore, radiation induced more TGF-β1EV secretion and higher percentage of Tregs by promoting the expression and phosphorylation of protein kinase C zeta (PKC-ζ). Importantly, we found that naringenin rather than 1D11 significantly improved radiotherapy efficacy with less side effects. Distinct from TGF-β1 neutralizing antibody 1D11, the mechanism of naringenin was to downregulate the radiation-activated superoxide-Zinc-PKC-ζ-TGF-β1EV pathway.

Conclusions
The superoxide-zinc-PKC-ζ-TGF-β1EV release pathway was elucidated to induce the accumulation of Tregs, resulting in radiotherapy resistance in the TME. Therefore, targeting PKC-ζ to counteract TGF-β1EV function could represent a novel strategy to overcome radiotherapy resistance in the treatment of breast cancer or other cancers.
Trial registration: The using of patient tissues with malignant Non-Small Cell Lung Cancer (NSCLC) was approved by the ethics committees at Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China (NCC2022C-702, from June 8th, 2022).
Synthesis and adsorption performance of functionalized chitosan and carboxyethylsilanetriol hybrids
Authors: Salama Ahmed , El-Sakhawy Mohamed 
Published: April 2023

AbstractA novel adsorbent from cationic chitosan derivative and anionic silica precursor was fabricated to remove methylene blue (MB). The hybrid material was prepared from N-guanidinium chitosan acetate (GChi) and carboxyethylsilanetriol sodium salt by a simple ionic interaction followed by sol–gel approach. Multiple characterization methods were used to analyze the morphology and the structure of the well-prepared functionalized material. Batch experiments were conducted to optimize the various operational parameters. The Langmuir isotherm was used to fit the data, and it predicted monolayer adsorption with a maximum capacity of 334 mg g−1. A pseudo-second-order equation fit the adsorption process well. Chitosan/silica hybrids containing carboxylic groups are efficient and cost-effective adsorbents for cationic dyes adsorption from aqueous solutions.
QSAR and molecular docking studies of isatin and indole derivatives as SARS 3CLpro inhibitors
Authors: Soleymani Niousha , Ahmadi Shahin , Shiri Fereshteh , Almasirad Ali 
Published: April 2023

AbstractThe 3C-like protease (3CLpro), known as the main protease of SARS-COV, plays a vital role in the viral replication cycle and is a critical target for the development of SARS inhibitor. Comparative sequence analysis has shown that the 3CLpro of two coronaviruses, SARS-CoV-2 and SARS-CoV, show high structural similarity, and several common features are shared among the substrates of 3CLpro in different coronaviruses. The goal of this study is the development of validated QSAR models by CORAL software and Monte Carlo optimization to predict the inhibitory activity of 81 isatin and indole-based compounds against SARS CoV 3CLpro. The models were built using a newer objective function optimization of this software, known as the index of ideality correlation (IIC), which provides favorable results. The entire set of molecules was randomly divided into four sets including: active training, passive training, calibration and validation sets. The optimal descriptors were selected from the hybrid model by combining SMILES and hydrogen suppressed graph (HSG) based on the objective function. According to the model interpretation results, eight synthesized compounds were extracted and introduced from the ChEMBL database as good SARS CoV 3CLpro inhibitor. Also, the activity of the introduced molecules further was supported by docking studies using 3CLpro of both SARS-COV-1 and SARS-COV-2. Based on the results of ADMET and OPE study, compounds CHEMBL4458417 and CHEMBL4565907 both containing an indole scaffold with the positive values of drug-likeness and the highest drug-score can be introduced as selected leads.
Comprehensive analyses of molecular features, prognostic values, and regulatory functionalities of m6A-modified long non-coding RNAs in lung adenocarcinoma
Authors: Ping Yili , Huang Jingjuan , Zhu Jichao , Sun Zujun , Shang Anquan , Chen Chen , Liu Wenfang , Li Dong 
Published: April 2023

Abstract
Background
Lung adenocarcinoma (LUAD) has a high incidence and recurrence rate. N6-methyladenosine (m6A) modification of RNA has become a promising epigenetic marker in tumors. The dysregulation of both RNA m6A levels and m6A regulator expression levels reportedly affects essential biological processes in various tumors. Long non-coding RNAs (lncRNAs), a subgroup of RNAs over 200 nucleotides in length that do not code for protein, can be modified and regulated by m6A, but the relevant profile in LUAD remains unclear.

Results
The m6A levels of total RNA were decreased in LUAD tumor tissues and cells. Multiple m6A regulators were abnormally expressed at both the RNA and protein levels, and were related in expression patterns and functionally synergistic. Our microarray revealed 2846 m6A-modified lncRNA transcripts as well as its molecular features, 143 of which were differentially m6A-modified and manifested a negative correlation between expression levels and m6A modification levels. More than half of the differentially m6A-modified lncRNAs associated with dysregulated expression. The 6-MRlncRNA risk signature was a reliable indicator for assessing survival time of LUAD patients. The competitive endogenous regulatory network suggested a potential m6A-induced pathogenicity in LUAD.

Conclusions
These data have demonstrated that differential RNA m6A modification and m6A regulator expression levels were identified in LUAD patients. In addition, this study provides evidence increasing the understanding of molecular features, prognostic values, and regulatory functionalities of m6A-modified lncRNAs in LUAD.
Presacral malakoplakia presenting as foot drop: a case report
Authors: Yates Tom A. , Devlin Katie , Arnaout Abed , Hurt William , Stone Neil , Everett Kate V. , Pittman Alan , Patel Hardik , Heenan Susan , Hart Paul , Harrison Thomas S. 
Published: April 2023

Abstract
Background
Malakoplakia is a rare condition characterized by inflammatory masses with specific histological characteristics. These soft tissue masses can mimic tumors and tend to develop in association with chronic or recurrent infections, typically of the urinary tract. A specific defect in innate immunity has been described. In the absence of randomized controlled trials, management is based on an understanding of the biology and on case reports.

Case presentation
Here we describe a case of presacral malakoplakia in a British Indian woman in her late 30s, presenting with complex unilateral foot drop. Four years earlier, she had suffered a protracted episode of intrapelvic sepsis following a caesarean delivery. Resection of her presacral soft tissue mass was not possible. She received empiric antibiotics, a cholinergic agonist, and ascorbic acid. She responded well to medical management both when first treated and following a recurrence of symptoms after completing an initial 8 months of therapy. Whole exome sequencing of the patient and her parents was undertaken but no clear causal variant was identified.

Conclusions
Malakoplakia is uncommon but the diagnosis should be considered where soft tissue masses develop at the site of chronic or recurrent infections. Obtaining tissue for histological examination is key to making the diagnosis. This case suggests that surgical resection is not always needed to achieve a good clinical and radiological outcome.
Coronavirus disease 2019-associated nephropathy in an African American patient: a case report and review of the literature
Authors: Dhillon Vijaypal S. , Alkashash Ahmad , Viquez-Beita Karolina 
Published: April 2023

Abstract
Background
Acute kidney injury is now recognized as a common complication of coronavirus disease 2019, affecting up to 46% of patients, with acute tubular injury as the most common etiology. Recently, we have seen an increase in cases of collapsing glomerulonephritis in patients with coronavirus disease 2019, also known as coronavirus disease 2019-associated nephropathy. It has been noted to be seen with a higher incidence in African American patients who are carriers of the APOL1 variant allele.

Case presentation
A 47-year-old African American male with a past medical history of asthma presented to the emergency department with complaints of intermittent chest pain, shortness of breath, and worsening confusion. On admission, he was found to be hemodynamically stable, but labs were significant for elevated creatinine and blood urea nitrogen, signifying acute kidney injury. He was admitted and taken for emergent dialysis. During his hospitalization, he was found to be positive for coronavirus disease 2019. Renal biopsy was done, which showed collapsing glomerulopathy, and the patient continues to require outpatient dialysis after discharge.

Conclusion
Collapsing glomerulonephritis has emerged as a complication in patients with coronavirus disease 2019. This condition should be particularly suspected in African American patients who present with acute kidney injury, nephrotic-range proteinuria, and who are positive for coronavirus disease 2019. Current treatment options are limited to supportive treatment and renal replacement therapy. More clinical cases and trials are needed to better understand and improve therapeutic outcomes in these patients.
Bioengineered tissue and cell therapy products are efficiently cryopreserved with pathogen-inactivated human platelet lysate-based solutions
Authors: Martín-López María , Rosell-Valle Cristina , Arribas-Arribas Blanca , Fernández-Muñoz Beatriz , Jiménez Rosario , Nogueras Sonia , García-Delgado Ana Belén , Campos Fernando , Santos-González Mónica 
Published: April 2023

Abstract
Background
There remains much interest in improving cryopreservation techniques for advanced therapy medicinal products (ATMPs). Recently, human platelet lysate (hPL) has emerged as a promising candidate to replace fetal bovine serum (FBS) as a xeno-free culture supplement for the expansion of human cell therapy products. Whether hPL can also substitute for FBS in cryopreservation procedures remains poorly studied. Here, we evaluated several cryoprotective formulations based on a proprietary hPL for the cryopreservation of bioengineered tissues and cell therapy products.

Methods
We tested different xenogeneic-free, pathogen-inactivated hPL (ihPL)- and non-inactivated-based formulations for cryopreserving bioengineered tissue (cellularized nanostructured fibrin agarose hydrogels (NFAHs)) and common cell therapy products including bone marrow-derived mesenchymal stromal cells (BM-MSCs), human dermal fibroblasts (FBs) and neural stem cells (NSCs). To assess the tissue and cellular properties post-thaw of NFAHs, we analyzed their cell viability, identity and structural and biomechanical properties. Also, we evaluated cell viability, recovery and identity post-thaw in cryopreserved cells. Further properties like immunomodulation, apoptosis and cell proliferation were assessed in certain cell types. Additionally, we examined the stability of the formulated solutions. The formulations are under a bidding process with MD Bioproducts (Zurich, Switzerland) and are proprietary.

Results
Amongst the tissue-specific solutions, Ti5 (low-DMSO and ihPL-based) preserved the viability and the phenotype of embedded cells in NFAHs and preserved the matrix integrity and biomechanical properties similar to those of the standard cryopreservation solution (70% DMEM + 20% FBS + 10% DMSO). All solutions were stable at − 20 °C for at least 3 months. Regarding cell-specific solutions, CeA maintained the viability of all cell types > 80%, preserved the immunomodulatory properties of BM-MSCs and promoted good recovery post-thaw. Besides, both tested solutions were stable at − 20 °C for 18 months. Finally, we established that there is a 3-h window in which thawed NFAHs and FBs maintain optimum viability immersed in the formulated solutions and at least 2 h for BM-MSCs.

Conclusions
Our results show that pathogen-inactivated solutions Ti5 allocated for bioengineered tissues and CeA allocated for cells are efficient and safe candidates to cryopreserve ATMPs and offer a xenogeneic-free and low-DMSO alternative to commercially available cryoprotective solutions.
Human adipose tissue-derived small extracellular vesicles promote soft tissue repair through modulating M1-to-M2 polarization of macrophages
Authors: Dong Jia , Wu Bin , Tian Weidong 
Published: April 2023

AbstractBackgroundSuccessful regenerative medicine strategies need the manipulation and control of macrophages’ phenotypic switching. Our previous study indicated that rat and porcine adipose tissue-derived small extracellular vesicles could successfully promote soft tissue repair. However, whether human adipose tissue-derived small extracellular vesicles (h-sEV-AT) showed the same ability to promote soft tissue regeneration and whether adipose tissue-derived small extracellular vesicles (sEV-AT) contribute to modulating the polarization of macrophages were unknown.MethodsIn this study, we, for the first time, isolated h-sEV-AT from liposuction adipose tissue and characterized the morphology, size distribution, and marker protein. In vitro, we treated adipose-derived stromal/stem cells (ASCs), endothelial cells (ECs), and M1 macrophages with h-sEV-AT. In vivo, the ability of h-sEV-AT to promote soft tissue regeneration and polarize macrophages was investigated.ResultsThe results indicated that h-sEV-AT possessed the characteristics of small extracellular vesicles (sEVs). In vitro, an obvious increase in adipogenesis and angiogenesis was induced by h-sEV-AT. In vivo, h-sEV-AT successfully induced the regeneration of adipose tissue and effectively accelerated full-thickness skin wound healing. Besides, we found that h-sEV-AT showed the ability to increase the percentage of M2 macrophages both in vivo and in vitro, which had been reported to contribute to tissue repair and regeneration.ConclusionsTaken together, these results suggested that h-sEV-AT showed the ability to induce soft tissue repair supported by not only the differentiation of ASCs and ECs but also the polarization of macrophages. Considering the abundant sources, high yield, and guaranteed effectiveness, this study provided a cell-free strategy for soft tissue regeneration that directly isolated small extracellular vesicles from human liposuction adipose tissue.
An improved monarch butterfly spectrum allocation algorithm for multi-source data stream in complex electromagnetic environment
Authors: Liu Yuchao , Cao Chenggang , Han Yu 
Published: April 2023

AbstractIn the era of the Internet of Everything, various wireless devices and sensors use spectrum, which is a precious and non-renewable resource, to communication. Due to the characteristics of massive, heterogeneous, and multi-source, the generated multi-source data stream brings difficulties to spectrum cognition. As a result, unreasonable spectrum allocation strategy leads to low utilization of spectrum resources. Optimizing spectrum allocation strategy can effectively improve spectrum utilization. Aiming at the problem of trapped local optimum solution in the genetic algorithm (GA) and particle swarm optimization algorithm (PSO), an improved monarch butterfly algorithm is proposed. Firstly, this paper employs the simulated annealing algorithm to select the migration rate, which increases the diversity of monarch butterfly population. Secondly, chaos mapping algorithm is utilized to improve the optimization ability and convergence speed. Finally, in the view of the problem that the monarch butterfly algorithm is easy to fall into the local optimal solution, there is no better way to escape from the local optimal solution. The Wolf pack updating operator is selected to improve the diversity of the population to generate new monarch butterflies. This method updates the population by generating new monarch butterfly individuals, so as to increasing the diversity of the population. The experimental results show that the improved monarch butterfly algorithm outperforms the other two algorithms in terms of convergence speed and system revenue.
Effective path-loss compensation model based on multipath exploitation for through-the-wall radar imaging
Authors: Mkemwa Florian , Abdalla Abdi T. , Maiseli Baraka , Amour Idrissa , Muqaibel Ali 
Published: April 2023

AbstractThrough-the-wall radar imaging (TWRI) has attracted a great deal of attention in several sensitive applications, including rescue missions and military operations. Notwithstanding its broad range of applications, TWRI suffers from path-loss because distant targets experience more attenuation of signal power than those closer to the transceiver. This challenge may lead to missed targets with important information necessary for analysis and informed decision making. Responding to the challenge, we have developed a signal model with an effective path-loss compensator incorporating a free space exponent. Furthermore, multipath exploitation and compressive sensing techniques were employed to develop an effective algorithm for isolating residual clutter that may corrupt real targets. The proposed signal model integrates contributions from the front wall, multipath returns, and path-loss. Compared with the state-of-the-art model under the same experimental conditions, simulation results show that the proposed model achieves improved signal-to-clutter ratio, relative clutter peak, and probability of detection by 13.1%, 17.4% and 33.6%, respectively, suggesting that our model can represent the scene more accurately.
Approximate controllability of second-order impulsive neutral stochastic differential equations with state-dependent delay and Poisson jumps
Authors: Huang Hao , Wu Zheng , Su Xiaofeng 
Published: April 2023

AbstractWe consider the approximate controllability for a class of second-order impulsive neutral stochastic differential equations with state-dependent delay and Poisson jumps in a real separable Hilbert space. Under the sufficient conditions, we obtain approximate controllability results by virtue of the theory of a strongly continuous cosine family of bounded linear operators combined with stochastic inequality technique and the Sadovskii fixed point theorem. Finally, we illustrate the main results by an example.
Response patterns of simulated corn yield and soil nitrous oxide emission to precipitation change
Authors: Kaur Navneet , Hui Dafeng , Riccuito Daniel M. , Mayes Melanie A. , Tian Hanqin 
Published: April 2023

Abstract
Background
Precipitation plays an important role in crop production and soil greenhouse gas emissions. However, how crop yield and soil nitrous oxide (N2O) emission respond to precipitation change, particularly with different background precipitations (dry, normal, and wet years), has not been well investigated. In this study, we examined the impacts of precipitation changes on corn yield and soil N2O emission using a long-term (1981–2020, 40 years) climate dataset as well as seven manipulated precipitation treatments with different background precipitations using the DeNitrification-DeComposition (DNDC) model.

Results
Results showed large variations of corn yield and precipitation but small variation of soil N2O emission among 40 years. Both corn yield and soil N2O emission showed near linear relationships with precipitation based on the long-term precipitation data, but with different response patters of corn yield and soil N2O emission to precipitation manipulations. Corn yield showed a positive linear response to precipitation manipulations in the dry year, but no response to increases in precipitation in the normal year, and a trend of decrease in the wet year. The extreme drought treatments reduced corn yield sharply in both normal and wet years. In contrast, soil N2O emission mostly responded linearly to precipitation manipulations. Decreases in precipitation in the dry year reduced more soil N2O emission than those in the normal and wet years, while increases in precipitation increased more soil N2O emission in the normal and wet years than in the dry year.

Conclusions
This study revealed different response patterns of corn yield and soil N2O emission to precipitation and highlights that mitigation strategy for soil N2O emission reduction should consider different background climate conditions.