bluuebunny/crossref_metadata_2025_split huggingface dataset:
Analysis of molecular characteristics of CAMP-negative Streptococcus agalactiae strains
Authors: Zhou Jie , Zhang Li , Zhang Yang , Liu Hui , Xu Kangli , Zhang Baohu , Feng Tianyuan , Yang Shucai 
Published: May 2023

BackgroundStreptococcus agalactiae can produce CAMP factor, which can promote the β-hemolysin activity of Staphylococcus aureus, forming an arrow-shaped hemolysis enhancement zone at the intersection of the two bacterial species on a blood agar plate. This characteristic feature of Streptococcus agalactiae has led to the widespread use of the CAMP test as an identification method.MethodsVaginal/rectal swabs, collected from women at 35–37  weeks of pregnancy, were first inoculated into a selective enrichment broth media, then subcultured onto GBS chromogenic agar and 5% sheep blood agar sequentially. The VITEK-2 automatic identification system and MALDI-TOF MS were initially employed for identification, followed by the CAMP test. CAMP-negative strains underwent 16S rDNA and cfb gene sequence analysis, as well as bacterial multilocus sequence typing.ResultsA total of 190 strains were isolated, with 15 identified as CAMP-negative. Further 16S rDNA gene sequence analysis confirmed that all 15 strains were Streptococcus agalactiae. The MLST typing assay revealed that these 15 strains were of the ST862 type. The cfb gene was amplified and electrophoresed, but no specific fragments were found, indicating that these strains lack the CAMP factor due to cfb gene deletion. Antibiotic susceptibility tests demonstrated no resistance to penicillin, ampicillin, vancomycin and linezolid among the GBS strains. However, there are significant differences in resistance rates to tetracycline.ConclusionThis study found that 7.9% of GBS strains isolated from the vagina/rectum of pregnant women were CAMP-negative, suggesting that the CAMP test method or primers targeting the cfb gene should not be used as the sole presumptive test for GBS identification.
Effectiveness and tolerability of perampanel monotherapy in children with newly diagnosed focal epilepsy
Authors: Zhao Fen , Ren Ying , Geng Guifu , Zhang Tong , Hu Wandong , Zhang Huan , Jin Ruifeng , Shi Jianguo , Gao Zaifen , Zhang Hongwei , Liu Yong 
Published: May 2023

ObjectiveTo examine the clinical effectiveness and tolerability of perampanel (PER) as initial monotherapy in pediatric patients with newly diagnosed focal epilepsy.MethodsA retrospective analysis was conducted on 62 children with newly diagnosed focal epilepsy who were treated with PER at the Epilepsy Center of Jinan Children's Hospital from July 2021 to July 2022. The treatment status, prognosis, and adverse reactions were followed up for a minimum of 6 months after the initiation of PER monotherapy. The effectiveness of the patients was estimated by the PER effective rate at 3-, 6-, and 12-month follow-up evaluations and adverse reactions were also recorded. The effective rates of PER in different etiologies and epilepsy syndromes were also statistically analyzed.ResultsThe effective rates of PER treatment at the different time points of evaluation were 88.7% (3 months), 79.1% (6 months), and 80.4% (12 months). With PER treatment, seizure freedom varied over time, with 61.3%, 71.0%, and 71.7% of patients at the 3-, 6-, and 12-month follow-ups, respectively. Among the etiologies of epilepsy, the effective rates of genetic etiology, structural etiology, and unknown etiology were generally above 50% at the 3-, 6-, and 12-month follow-ups. Among the epilepsy syndromes, the categories with higher treatment efficacy were self-limiting epilepsy with centrotemporal spikes (SeLECTs), self-limited epilepsy with autonomic seizures (SeLEAS), and childhood occipital visual epilepsy (COVE), with an effective rate of above 80%. Adverse events were documented in 22 patients (35.5%), but they were mild and tolerable. The most common adverse events comprised irritability, drowsiness, dizziness, and increased appetite.ConclusionPER has favorable effectiveness and tolerability as initial monotherapy for children with newly diagnosed focal epilepsy, which could be a potential option for long-term medication in the treatment of focal epilepsy in children. The current study provided potential evidence for PER as initial monotherapy in children with focal epilepsy in clinical practice.
Loss or gain of function? Effects of ion channel mutations on neuronal firing depend on the neuron type
Authors: Koch Nils A. , Sonnenberg Lukas , Hedrich Ulrike B. S. , Lauxmann Stephan , Benda Jan 
Published: May 2023

IntroductionClinically relevant mutations to voltage-gated ion channels, called channelopathies, alter ion channel function, properties of ionic currents, and neuronal firing. The effects of ion channel mutations are routinely assessed and characterized as loss of function (LOF) or gain of function (GOF) at the level of ionic currents. However, emerging personalized medicine approaches based on LOF/GOF characterization have limited therapeutic success. Potential reasons are among others that the translation from this binary characterization to neuronal firing is currently not well-understood—especially when considering different neuronal cell types. In this study, we investigate the impact of neuronal cell type on the firing outcome of ion channel mutations.MethodsTo this end, we simulated a diverse collection of single-compartment, conductance-based neuron models that differed in their composition of ionic currents. We systematically analyzed the effects of changes in ion current properties on firing in different neuronal types. Additionally, we simulated the effects of known mutations in KCNA1 gene encoding the KV1.1 potassium channel subtype associated with episodic ataxia type 1 (EA1).ResultsThese simulations revealed that the outcome of a given change in ion channel properties on neuronal excitability depends on neuron type, i.e., the properties and expression levels of the unaffected ionic currents.DiscussionConsequently, neuron-type specific effects are vital to a full understanding of the effects of channelopathies on neuronal excitability and are an important step toward improving the efficacy and precision of personalized medicine approaches.
Gene expression changes in cerebellum induced by dietary restriction
Authors: van’t Sant Lisanne J. , Birkisdóttir María B. , Ozinga Rutger A. , Gyenis Ákos , Hoeijmakers Jan H.J. , Vermeij Wilbert P. , Jaarsma Dick 
Published: May 2023

BackgroundDietary restriction (DR) is a well-established universal anti-aging intervention, and is neuroprotective in multiple models of nervous system disease, including models with cerebellar pathology. The beneficial effects of DR are associated with a rearrangement of gene expression that modulate metabolic and cytoprotective pathways. However, the effect of DR on the cerebellar transcriptome remained to be fully defined.ResultsHere we analyzed the effect of a classical 30% DR protocol on the transcriptome of cerebellar cortex of young-adult male mice using RNAseq. We found that about 5% of expressed genes were differentially expressed in DR cerebellum, the far majority of whom showing subtle expression changes. A large proportion of down-regulated genes are implicated in signaling pathways, in particular pathways associated with neuronal signaling. DR up regulated pathways in large part were associated with cytoprotection and DNA repair. Analysis of the expression of cell-specific gene sets, indicated a strong enrichment of DR down genes in Purkinje cells, while genes specifically associated with granule cells did not show such a preferential down-regulation.ConclusionOur data show that DR may have a clear effect on the cerebellar transcriptome inducing a mild shift from physiology towards maintenance and repair, and having cell-type specific effects.
Initial low-dose computed tomography screening results and summary of participant characteristics: based on the latest Chinese guideline
Authors: Zhang Zixuan , Gao Yinyan , Liu Shaohui , Ding Binrong , Zhang Xuewei , Wu Irene X. Y. 
Published: May 2023

BackgroundLow-dose computed tomography (LDCT) has been promoted as a promising screening strategy for early detection of lung cancer. China released the latest lung cancer screening guideline in 2021. The compliance of the individuals who received LDCT for lung cancer screening with the guideline is unknown yet. It is necessary to summarize the distribution of guideline-defined lung cancer–related risk factors in the Chinese population so as to inform the selection of target population for the future lung cancer screening.MethodsA single-center, cross-sectional study design was adopted. All participants were individuals who underwent LDCT at a tertiary teaching hospital in Hunan, China, between 1 January and 31 December 2021. LDCT results were derived along with guideline-based characteristics for descriptive analysis.ResultsA total of 5,486 participants were included. Over one-quarter (1,426, 26.0%) of the participants who received screening did not meet the guideline-defined high-risk population, even among non-smokers (36.4%). Most of the participants (4,622, 84.3%) were found to have lung nodules, while no clinical intervention was required basically. The detection rate of positive nodules varied from 46.8% to 71.2% when using different cut-off values for positive nodules. Among non-smoking women, ground glass opacity appeared to be more significantly common compared with non-smoking men (26.7% vs. 21.8%).ConclusionOver one-quarter of individuals who received LDCT screening did not meet the guideline-defined high-risk populations. Appropriate cut-off values for positive nodules need to be continuously explored. More precise and localized criteria for high-risk individuals are needed, especially for non-smoking women.
Erdafitinib treatment in metastatic urothelial carcinoma: a real-world analysis
Authors: Rouvinov Keren , Levanon Eran , Peer Avivit , Sarfaty Michal , Sarid David , Neiman Victoria , Grikshtas Eduard , Rosenbaum Eli , Kushnir Igal , Talmor Barak , Friger Michael , Zarbiv Yonaton , Gez Eli , Dresler Hadas , Shalata Walid , Meirovitz Amichay , Shrem Noa Shani , Yakobson Alexander , Mermershtain Wilmosh , Keizman Daniel 
Published: May 2023

BackgroundErdafitinib, a fibroblast growth factor receptor (FGFR) inhibitor is a standard post chemotherapy advanced treatment line for metastatic urothelial carcinoma harboring FGFR2/3 genomic alterations. It was approved based on a phase 2 clinical trial, revealing a 40% response rate, and 13.8 months overall survival. These FGFR genomic alterations are uncommon. Thus, real-world data on erdafitinb use is scant. We herein describe erdafitinib treatment outcome in a real world patient cohort.MethodsWe retrospectively reviewed the data of patients treated with erdafitinib from 9 Israeli medical centers.ResultsTwenty-five patients with metastatic urothelial carcinoma (median age 73, 64% male, 80% with visceral metastases) were treated with erdafitinib between January 2020 to October 2022. A clinical benefit (complete response 12%, partial response 32%, stable disease 12%) was seen in 56%. Median progression-free survival was 2.7 months, and median overall survival 6.73 months. Treatment related toxicity ≥ grade 3 occurred in 52%, and 32% discontinued therapy due to adverse events.ConclusionsErdafitinib therapy is associated with a clinical benefit in the real world setting, and associated with similar toxicity as reported in prospective clinical trials.
Relationship status and gender-related differences in response to infidelity
Authors: Kato Tsukasa , Okubo Nobutoshi 
Published: May 2023

IntroductionThe sexual imagination hypothesis suggests that responses to a partner’s infidelity emerge from the sociocultural factors that affect individuals’ imagining of that occurrence irrespective of biological sex, including relationship status (i.e., the experience of a serious, committed relationship). Nevertheless, evolutionary psychological perspectives predict that responses to a partner’s infidelity emerge from a sex-specific evolved innate mechanism.MethodsA lower 2D:4D digit ratio is associated with more robust responses to a partner’s sexual infidelity. In this study, participants (660 males and 912 females) were requested to measure finger lengths, reactions to their partners’ sexual and emotional infidelity, and relationship status.ResultsA logistic regression and multiple regression analyses revealed that relationship status was uniquely associated with responses to a partner’s sexual and emotional infidelity beyond the effects of sex and 2D:4D. Those in committed relationships were more upset or distressed over their partners’ infidelity, particularly over sexual infidelity, than those not in committed relationships.DiscussionThe results supported the sexual imagination hypothesis indirectly, while evolutionary psychological perspectives were met with skepticism. Our findings implied that sex differences in jealousy result from relationship status, and that responses to partners’ infidelity are more alike than different.
Detecting ulterior motives from verbal cues in group deliberations
Authors: Dunbar Norah E. , Burgoon Judee K. , Chen Xunyu , Wang Xinran , Ge Saiying , Huang Qing , Nunamaker Jay 
Published: May 2023

IntroductionForensic interviewing entails practitioners interviewing suspects to secure valid information and elicit confessions. Such interviews are often conducted in police stations but may also occur in field settings such as border crossings, security checkpoints, bus terminals, and sports venues. Because these real-world interviews often lack experimental control and ground truth, this investigation explored whether results of non-forensic interviews generalize to forensic ones.MethodsOrganizational espionage was simulated to determine (1) what verbal signals distinguish truth from deception, (2) whether deception in groups aligns with deception in dyads, and (3) whether non-forensic venues can be generalized to forensic ones. Engaging in a mock hiring deliberation, participants (4–5 strangers) reviewed and discussed resumes of potential candidates. Surreptitiously, two group members assigned the role of “organizational spies” attempted to persuade the group to hire an inferior candidate. Each group member presented notes from an interview of “their” candidate, followed by a discussion of all candidates. Spies were to use any means possible, including deception, to persuade others to select their candidate. A financial incentive was offered for having one’s candidate chosen. The interview reports and discussions were transcribed and analyzed with SPLICE, an automated text analysis program.ResultsDeceivers were perceived as less trustworthy than truth-tellers, especially when the naïve players won but overall, deceivers were difficult for non-spies to detect even though they were seen as less trustworthy than the naïve participants. Deceivers’ language was more complex and exhibited an “echoing” strategy of repeating others’ opinions. This collusion evolved naturally, without pre-planning. No other verbal differences were evident, which suggests that the difference between spies and non-spies was subtle and difficult for truth-tellers to spot.DiscussionWhether deception can be successfully detected hinges on a variety of factors including the deceiver’s skill to disguise and the detector’s ability to sense and process information. Furthermore, the group dynamics and communication context subtly moderate how deception manifests and influence the accuracy of detecting ulterior motives. Our future investigations could encompass non-verbal communication channels and verbal patterns rooted in content, thereby providing a more comprehensive understanding of deception detection.
The impact of early factors on persistent negative symptoms in youth at clinical high risk for psychosis
Authors: Devoe Daniel J. , Lui Lu , Cannon Tyrone D. , Cadenhead Kristin Suzanne , Cornblatt Barbara A. , Keshavan Matcheri , McGlashan Tom H. , Perkins Diana. O. , Seidman Larry J. , Stone William S. , Tsuang Ming T. , Woods Scott W. , Walker Elaine F. , Mathalon Daniel H. , Bearden Carrie E. , Addington Jean 
Published: May 2023

IntroductionPersistent negative symptoms (PNS) are described as continuing moderate negative symptoms. More severe negative symptoms have been associated with poor premorbid functioning in both chronic schizophrenia and first episode psychosis patients. Furthermore, youth at clinical high risk (CHR) for developing psychosis may also present with negative symptoms and poor premorbid functioning. The aim of this current study was to: (1) define the relationship between PNS and premorbid functioning, life events, trauma and bullying, previous cannabis use, and resource utilization, and (2) to examine what explanatory variables best predicted PNS.MethodCHR participants (N = 709) were recruited from the North American Prodrome Longitudinal Study (NAPLS 2). Participants were divided into two groups: those with PNS (n = 67) versus those without PNS (n = 673). A K-means cluster analysis was conducted to distinguish patterns of premorbid functioning across the different developmental stages. The relationships between premorbid adjustment and other variables were examined using independent samples t-tests or chi square for categorical variables.ResultsThere was significantly more males in the PNS group. Participants with PNS had significantly lower levels of premorbid adjustment in childhood, early adolescence, and late adolescence, compared to CHR participants without PNS. There were no differences between the groups in terms of trauma, bullying, and resource utilization. The non-PNS group had more cannabis use and more desirable and non-desirable life events.ConclusionIn terms of better understanding relationships between early factors and PNS, a prominent factor associated with PNS was premorbid functioning, in particular poor premorbid functioning in later adolescence.
Toward an integrative socio-cognitive approach in autism spectrum disorder: NEAR method adaptation—study protocol
Authors: Bahri Jihene , Abbes Zeineb S. , Ben Yahia Houda , Halayem Soumeyya , Jelili Selima , Hajri Melek , Amado Isabelle , Medalia Alice , Bouden Asma 
Published: May 2023

BackgroundThe cognitive impairments exhibited by people with ASD, threaten the development of social skills that are essential for establishing and maintaining harmonious social relationships. Cognitive remediation and social skills training are now considered as crucial therapeutic approaches in the management of these disorders. Several programs have already been validated and have shown improvements in social skills or cognitive performance. However, the effects of these training methods seem to be difficult to generalize to other everyday life. The aim of our study is to alleviate cognitive and social deficiencies by using a socio-cognitive framework to adapt the Neuropsychological Educational Approach to Remediation (NEAR) method for adolescents with ASD.Methods/designAdolescents meeting the DSM-5 criteria for ASD, older than 13 years, and following a regular school curriculum will be recruited from clinical population at the Child and Adolescent Psychiatry in Razi University Hospital-Manouba- Tunisia. Our study is an open and non-randomized controlled trial including 30 patients: NEAR group / control group. The NEAR method combines computerized cognitive exercises and bridging groups inspired from cognitive behavioral therapy. NEAR group will be divided into three groups of five patients each. The duration of the sessions will vary according to the capacities of the participants and the exchanges between them (about 60–120 min). In our study, bridging groups will be amended by adding other tasks including planning role plays and scenarios of problematic social situations in autism, taking into account cultural particularities in order to promote social skills. Computerized exercises will be enriched by adding other tasks aiming to improve the recognition and expression of facial emotions by using digital videos and photographs expressing the six basic emotions. The duration of the program will be about 6 months. All selected patients will have an assessment of cognitive function: social cognition, neurocognition and pragmatic skills, social skills, self-esteem and global functioning at baseline, 1 week after the end of the NEAR program and 6 months later.ConclusionThis adaptive program is a promising socio-cognitive intervention that create new perspectives for adolescents with autism spectrum disorder.
Feasibility of implementing public-private mix approach for tuberculosis case management in Pokhara Metropolitan City of western Nepal: a qualitative study
Authors: Baral Sushila , Yadav Rajesh Kumar , Yadav Dipendra Kumar , Marahatta Sujan Babu , Baral Yadunath , Khadka Khim Bahadur , Thakur Sanjay Kumar , Paudel Srijana , Sharma Prabin , Pandey Sony , Shrestha Kusum , Shah Naveen Prakash , Basaula Laxman , Nagila Amar , Mahato Roshan Kumar , Ranabhat Chhabi Lal 
Published: May 2023

BackgroundThe Public-Private Mix (PPM) approach is a strategic initiative that involves engaging all private and public health care providers in the fight against tuberculosis using international health care standards. For tuberculosis control in Nepal, the PPM approach could be a milestone. This study aimed to explore the barriers to a public-private mix approach in the management of tuberculosis cases in Nepal.MethodsWe conducted key informant interviews with 20 participants, 14 of whom were from private clinics, polyclinics, and hospitals where the PPM approach was used, two from government hospitals, and four from policymakers. All data were audio-recorded, transcribed, and translated into English. The transcripts of the interviews were manually organized, and themes were generated and categorized into 1. TB case detection, 2. patient-related barriers, and 3. health-system-related barriers.ResultsA total of 20 respondents participated in the study. Barriers to PPM were identified into following three themes: (1) Obstacles related to TB case detection, (2) Obstacles related to patients, and (3) Obstacles related to health-care system. PPM implementation was challenged by following sub-themes that included staff turnover, low private sector participation in workshops, a lack of trainings, poor recording and reporting, insufficient joint monitoring and supervision, poor financial benefit, lack of coordination and collaboration, and non-supportive TB-related policies and strategies.ConclusionGovernment stakeholders can significantly benefit by applying a proactive role working with the private in monitoring and supervision. The joint efforts with private sector can then enable all stakeholders to follow the government policy, practice and protocols in case finding, holding and other preventive approaches. Future research are essential in exploring how PPM could be optimized.