bluuebunny/crossref_metadata_2025_split huggingface dataset:
A qualitative analysis of pharmacists’ attitudes towards provision of medication abortion
Authors: Sandoval Selina , Chen Grace , Rafie Sally , Rabin Borsika , Mody Sheila , Averbach Sarah 
Published: May 2023

Abstract
Background
90% of United States’ counties do not have a single clinic offering abortion care, and barriers to care disproportionately affect low-income families. Novel models of abortion care delivery, including provision of medication abortion in pharmacies, with pharmacists prescribing medication, have the potential to expand access to abortion care. Pharmacists are well-positioned to independently provide abortion care and are highly accessible to patients, however medication abortion provision by pharmacists is not currently legal or available in the United States. To assess the potential acceptability of pharmacist provision of medication abortion and to identify anticipated barriers and facilitators to this model of care, we explored pharmacists’ attitudes towards providing medication abortion, inclusive of patient selection, counseling, and medication prescribing.

Methods
From May to October 2021, we conducted 20 semi-structured qualitative interviews with pharmacists across the United States, guided by the domains of the Consolidated Framework for Implementation Science Research.

Results
Major themes included there is a need for pharmacist provision of medication abortion and pharmacists perceive provision of medication abortion to be potentially acceptable if anticipated barriers are addressed. Anticipated barriers identified included personal, religious, and political beliefs of pharmacists and lack of space and systems to support the model. Ensuring adequate staffing with pharmacists willing to participate, private space, time for counseling, safe follow-up, training, and reimbursement mechanisms were perceived strategies to facilitate successful implementation.

Conclusions
Pharmacist identified implementation strategies are needed to reduce anticipated barriers to pharmacist provision of medication abortion.
RNA sequencing and bioinformatics analysis of differentially expressed genes in the peripheral serum of ankylosing spondylitis patients
Authors: Bie Yongchen , Zheng Xiujun , Chen Xiaojiong , Liu Xiangyun , Wang Liqin , Sun Yuanliang , Kou Jianqiang 
Published: May 2023

Abstract
Background
Ankylosing spondylitis (AS) is a chronic progressive autoimmune disease characterized by spinal and sacroiliac arthritis, but its pathogenesis and genetic basis are largely unclear.

Methods
We randomly selected three serum samples each from an AS and a normal control (NC) group for high-throughput sequencing followed by using edgeR to find differentially expressed genes (DEGs). Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes, Reactome pathway analyses, and Gene Set Enrichment Analysis were used to comprehensively analyze the possible functions and pathways involved with these DEGs. Protein–protein interaction (PPI) networks were constructed using the STRING database and Cytoscape. The modules and hub genes of these DEGs were identified using MCODE and CytoHubba plugins. Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) was used to validate the expression levels of candidate genes in serum samples from AS patients and healthy controls.

Results
We successfully identified 100 significant DEGs in serum. When we compared them with the NC group, 49 of these genes were upregulated in AS patients and 51 were downregulated. GO function and pathway enrichment analysis indicated that these DEGs were mainly enriched in several signaling pathways associated with endoplasmic reticulum stress, including protein processing in the endoplasmic reticulum, unfolded protein response, and ubiquitin-mediated proteolysis. We also constructed a PPI network and identified the highly connected top 10 hub genes. The expression levels of the candidate hub genes PPARG, MDM2, DNA2, STUB1, UBTF, and SLC25A37 were then validated by RT-qPCR analysis. Finally, receiver operating characteristic curve analysis suggested that PPARG and MDM2 may be the potential biomarkers of AS.

Conclusions
These findings may help to further elucidate the pathogenesis of AS and provide valuable potential gene biomarkers or targets for the diagnosis and treatment of AS.
Depression, sexual function and sexual quality of life in women with polycystic ovary syndrome (PCOS) and healthy subjects
Authors: Mojahed Batool Shahraki , Ghajarzadeh Mahsa , Khammar Razie , Shahraki Zahra 
Published: May 2023

Abstract
Background
Women with polycystic ovary syndrome (PCOS) suffer from a wide range of psychological problems. The goal of this study is to assess depression, sexual dysfunction and sexual quality of life in women with PCO compared with healthy subjects.

Methods
One hundred and six PCO cases and 106 healthy subjects enrolled. They were asked to fill out valid and reliable Persian versions of BDI (Beck depression inventory), FSFIS (Female Sexual Function Index), and sexual quality of life-Female (SQOL-F) questionnaires.

Results
One hundred and six PCO cases and 106 healthy subjects enrolled. Mean BDI was significantly higher while FSFI and its subscales as well as SQOL-F were significantly lower in PCO group than controls. There were significant negative correlation between FSFO and BDI (r=-0.43, p < 0.001) and also positive correlation between FSFI and SQOL-F (r = 0.438, p < 0.001) in whole population of the study. In PCO group, 77 (72.6%) had FSFI less than 26.55 and 29 (27.4%) had score more than 26.55. mean BDI was significantly higher in the group with FSFI < = 26.55 than the other group (33 ± 14.4 vs. 12 ± 11.6, p < 0.001) while SLQL-F was significantly lower in the first group (57 ± 16 vs. 74.9 ± 17.6, p < 0.001). Linear regression analysis by considering SQOL-F as dependent and FSFI, age and BDI as independent variables showed that BDI and FSFI are independent predictors.

Conclusion
Women with PCO, suffer more from depression and sexual dysfunction than healthy subjects.
Head-to-head comparison of perfluorobutane contrast-enhanced US and multiparametric MRI for breast cancer: a prospective, multicenter study
Authors: Lang Manlin , Liang Ping , Shen Huiming , Li Hang , Yang Ning , Chen Bo , Chen Yixu , Ding Hong , Yang Weiping , Ji Xiaohui , Zhou Ping , Cui ligang , Wang Jiandong , Xu Wentong , Ye Xiuqin , Liu Zhixing , Yang Yu , Wei Tianci , Wang Hui , Yan Yuanyuan , Wu Changjun , Wu Yiyun , Shi Jingwen , Wang Yaxi , Fang Xiuxia , li Ran , Yu Jie 
Published: May 2023

AbstractBackgroundMultiparametric magnetic resonance imaging (MP-MRI) has high sensitivity for diagnosing breast cancers but cannot always be used as a routine diagnostic tool. The present study aimed to evaluate whether the diagnostic performance of perfluorobutane (PFB) contrast-enhanced ultrasound (CEUS) is similar to that of MP-MRI in breast cancer and whether combining the two methods would enhance diagnostic efficiency.Patients and methodsThis was a head-to-head, prospective, multicenter study. Patients with breast lesions diagnosed by US as Breast Imaging Reporting and Data System (BI-RADS) categories 3, 4, and 5 underwent both PFB-CEUS and MP-MRI scans. On-site operators and three reviewers categorized the BI-RADS of all lesions on two images. Logistic-bootstrap 1000-sample analysis and cross-validation were used to construct PFB-CEUS, MP-MRI, and hybrid (PFB-CEUS + MP-MRI) models to distinguish breast lesions.ResultsIn total, 179 women with 186 breast lesions were evaluated from 17 centers in China. The area under the receiver operating characteristic curve (AUC) for the PFB-CEUS model to diagnose breast cancer (0.89; 95% confidence interval [CI] 0.74, 0.97) was similar to that of the MP-MRI model (0.89; 95% CI 0.73, 0.97) (P = 0.85). The AUC of the hybrid model (0.92, 95% CI 0.77, 0.98) did not show a statistical advantage over the PFB-CEUS and MP-MRI models (P = 0.29 and 0.40, respectively). However, 90.3% false-positive and 66.7% false-negative results of PFB-CEUS radiologists and 90.5% false-positive and 42.8% false-negative results of MP-MRI radiologists could be corrected by the hybrid model. Three dynamic nomograms of PFB-CEUS, MP-MRI and hybrid models to diagnose breast cancer are freely available online.ConclusionsPFB-CEUS can be used in the differential diagnosis of breast cancer with comparable performance to MP-MRI and with less time consumption. Using PFB-CEUS and MP-MRI as joint diagnostics could further strengthen the diagnostic ability.Trial registrationClinicaltrials.gov; NCT04657328. Registered 26 September 2020.IRB number2020-300 was approved in Chinese PLA General Hospital. Every patient signed a written informed consent form in each center.
Exercise and epigenetic ages in older adults with myeloid malignancies
Authors: Loh Kah Poh , Sanapala Chandrika , Jensen-Battaglia Marielle , Rana Anish , Sohn Michael B. , Watson Erin , Gilmore Nikesha , Klepin Heidi D. , Mendler Jason H. , Liesveld Jane , Huselton Eric , LoCastro Marissa , Susiarjo Martha , Netherby-Winslow Colleen , Williams AnnaLynn M. , Mustian Karen , Vertino Paula , Janelsins Michelle C. 
Published: May 2023

Abstract
Background
Older adults with myeloid malignancies are susceptible to treatment-related toxicities. Accelerated DNAm age, or the difference between DNA methylation (DNAm) age and chronological age, may be used as a biomarker of biological age to predict individuals at risk. In addition, cancer treatment can also lead to accelerated DNAm age. Exercise is a promising intervention to reduce or prevent functional, psychological, and cognitive impairments in older patients with myeloid malignancies, yet there is little evidence of the effects of exercise on DNAm age. We explored (1) the associations of accelerated DNAm age with physical, psychological, and cognitive functions at baseline; (2) changes in DNAm age from baseline to post-intervention; and (3) the associations of changes in accelerated DNAm age with changes in functions from baseline to post-intervention.

Methods
We enrolled older patients with myeloid malignancies to a single-arm pilot study testing a mobile health (mHealth) exercise intervention that combines an exercise program (EXCAP©®) with a mobile application over 2 cycles of chemotherapy (8–12 weeks). Patients completed measures of physical, psychological, and cognitive functions and provided blood samples for analyses of DNAm age at baseline and post-intervention. Paired t-tests or Wilcoxon signed rank tests assessed changes in DNAm ages, and Spearman’s correlation assessed the relationships between accelerated ages and functions.

Results
We included 20 patients (mean age: 72 years, range 62–80). Accelerated GrimAge, accelerated PhenoAge, and DunedinPACE were stable from baseline to post-intervention. At baseline, DunedinPACE was correlated with worse grip strength (r = -0.41, p = 0.08). From baseline to post-intervention, decreases in accelerated GrimAge (r = -0.50, p = 0.02), accelerated PhenoAge (r = − 0.39, p = 0.09), and DunedinPace (r = − 0.43, p = 0.06) were correlated with increases in distance walked on 6-min walk test. Decreases in accelerated GrimAge (r = − 0.49, p = 0.03), accelerated PhenoAge (r = − 0.40, p = 0.08), and DunedinPace (r = − 0.41, p = 0.07) were correlated with increases in in grip strength.

Conclusions
Among older adults with myeloid malignancies receiving chemotherapy, GrimAge and PhenoAge on average are stable after a mHealth exercise intervention. Decreases in accelerated GrimAge, accelerated PhenoAge, and DunedinPACE over 8–12 weeks of exercise were correlated with increased physical performance. Future trials assessing the effects of exercise on treatment-related toxicities should evaluate DNAm age.
Trial registration Clinicaltrials.gov identifier: NCT04981821.
Akkermansia muciniphila, which is enriched in the gut microbiota by metformin, improves cognitive function in aged mice by reducing the proinflammatory cytokine interleukin-6
Authors: Zhu Xiaoqi , Shen Junyan , Feng Shengyu , Huang Ce , Wang Hao , Huo Fengjiao , Liu Hailiang 
Published: May 2023

Abstract
Background
Metformin, a type 2 diabetes treatment, improves the cognitive function of aged mice; however, whether the protective effects of metformin on cognitive function in aged mice are associated with the gut microbiome is poorly understood. Although some studies suggest that the gut microbe composition influences cognitive function and that manipulating the gut microbiota might protect against age-related cognitive dysfunction, there is no direct evidence to validate that the gut microbiota mediates the effect of metformin on cognitive improvement.

Results
In this study, we show that the gut microbiota is altered by metformin, which is necessary for protection against ageing-associated cognitive function declines in aged mice. Mice treated with antibiotics did not exhibit metformin-mediated cognitive function protection. Moreover, treatment with Akkermansia muciniphila, which is enriched by metformin, improved cognitive function in aged mice. Mechanistically, A. muciniphila decreased pro-inflammatory-associated pathways, particularly that of the pro-inflammatory cytokine interleukin (IL)-6, in both the peripheral blood and hippocampal profiles, which was correlated with cognitive function improvement. An IL-6 antibody protected cognitive function, and an IL-6 recombinant protein abolished the protective effect of A. muciniphila on cognitive function in aged mice.

Conclusion
This study reveals that A. muciniphila, which is mediated in the gut microbiota by metformin, modulates inflammation-related pathways in the host and improves cognitive function in aged mice by reducing the pro-inflammatory cytokine IL-6.

Graphical Abstract
The Cannabis sativa genetics and therapeutics relationship network: automatically associating cannabis-related genes to therapeutic properties through chemicals from cannabis literature
Authors: Jackson Trever J. , Chakraborty Sunandan 
Published: May 2023

Abstract
Background
Understanding the genome of Cannabis sativa holds significant scientific value due to the multi-faceted therapeutic nature of the plant. Links from cannabis gene to therapeutic property are important to establish gene targets for the optimization of specific therapeutic properties through selective breeding of cannabis strains. Our work establishes a resource for quickly obtaining a complete set of therapeutic properties and genes associated with any known cannabis chemical constituent, as well as relevant literature.

Methods
State-of-the-art natural language processing (NLP) was used to automatically extract information from many cannabis-related publications, thus producing an undirected multipartite weighted-edge paragraph co-occurrence relationship network composed of two relationship types, gene-chemical and chemical property. We also developed an interactive application to visualize sub-graphs of manageable size.

Results
Two hundred thirty-four cannabis constituent chemicals, 352 therapeutic properties, and 124 genes from the Cannabis sativa genome form a multipartite network graph which transforms 29,817 cannabis-related research documents from PubMed Central into an easy to visualize and explore network format.

Conclusion
Use of our network replaces time-consuming and labor intensive manual extraction of information from the large amount of available cannabis literature. This streamlined information retrieval process will enhance the activities of cannabis breeders, cannabis researchers, organic biochemists, pharmaceutical researchers and scientists in many other disciplines.
Impact of time to revision total knee arthroplasty on outcomes following aseptic failure
Authors: Roof Mackenzie A. , Narayanan Shankar , Lorentz Nathan , Aggarwal Vinay K. , Meftah Morteza , Schwarzkopf Ran 
Published: May 2023

Abstract
Introduction
Prior studies have demonstrated an association between time to revision total knee arthroplasty (rTKA) and indication; however, the impact of early versus late revision on post-operative outcomes has not been reported.

Materials and methods
A retrospective, observational study examined patients who underwent unilateral, aseptic rTKA at an academic orthopedic hospital between 6/2011 and 4/2020 with > 1-year of follow-up. Patients were early revisions if they were revised within 2 years of primary TKA (pTKA) or late revisions if revised after greater than 2 years. Patient demographics, surgical factors, and post-operative outcomes were compared.

Results
470 rTKA were included (199 early, 271 late). Early rTKA patients were younger by 2.5 years (p = 0.002). The predominant indications for early rTKA were instability (28.6%) and arthrofibrosis/stiffness (26.6%), and the predominant indications for late rTKA were aseptic loosening (45.8%) and instability (26.2%; p < 0.001). Late rTKA had longer operative times (119.20 ± 51.94 vs. 103.93 ± 44.66 min; p < 0.001). There were no differences in rTKA type, disposition, hospital length of stay, all-cause 90-day emergency department visits and readmissions, reoperations, and number of re-revisions.

Conclusions
Aseptic rTKA performed before 2 years had different indications but demonstrated similar outcomes to those performed later. Early revisions had shorter surgical times, which could be attributed to differences in rTKA indication.

Level of evidence
III, retrospective observational analysis.
Effects of Pilates on health and well-being of women: a systematic review
Authors: Parveen Afsha , Kalra Sheetal , Jain Shilpa 
Published: May 2023

AbstractBackgroundPilates (Contrology) is a mind–body exercise that emphasises strength, core stability, flexibility, muscle control, posture, and breathing. Pilates can be practised in a variety of methods, including using various equipment and one’s own body weight on a mat. This review’s objective is to assess the benefits of Pilates therapies for women with health issues, with a focus on physical and psychological health, with a focus on physical and psychosocial results.MethodologyAll published Randomised controlled trials (RCTs) and comparative trials with free full text that involved female participants with medical conditions with Pilates exercises as the intervention were included. A search was conducted across 3 databases (Google Scholar, PubMed, and Cochrane Library): 10 studies—7 RCTs and 3 comparative studies, met the inclusion criteria. The Cochrane risk of bias tool was used to evaluate the quality of the methodology.ResultsAccording to recent research, Pilates may improve quality of life while lowering pain, and disability. It may also increase flexibility, strength, mobility, respiratory rate, vital capacity, body mass index, and balance. It also helps in lowering fasting blood glucose, and HbA1c level in type 2 diabetic women and also helps in lowering the severity of temporomandibular dysfunction.ConclusionWomen with health issues who practised pilates reported improvements in physical and psychological health metrics. Additional high-quality research is necessary to determine the impact on other aspects of health and fitness.Trial registrationThis systematic review was registered on PROSPERO with a registration ID CRD42022328804.
Does COVID-19 vaccination protect against pulmonary embolism?
Authors: Tantillo Simona , Cilloni Nicola , Guarnera Martina , Talarico Francesco , Citino Mario , Silingardi Mauro , Catalano Leonardo , Imbriani Michele 
Published: May 2023

Abstract
The prevalence of venous thromboembolism (VTE) in COVID-19 patients is highly variable, depending on methodological and clinical factors, among which vaccination (1). The hypothesis of a possible protective role of vaccination in preventing pulmonary embolism (PE) in hospitalized COVID-19 patients has not been explored. The aim of the study was to evaluate PE prevalence in vaccinated versus unvaccinated hospitalized COVID-19 patients. We conducted a retrospective case–control study from 2021/11/01 to 2022/01/15; we reviewed all the chest computed topographies (chest-CT) performed because of a clinical suspicion for PE at our Institution. Sixty-two patients were included in the study: 27/62 (43.5%) were vaccinated and 35/62 (56.4%) were not. Vaccinated patients were older and with more comorbidities than unvaccinated people. Overall, PE was diagnosed in 19/62 patients (30.1% prevalence). CT Severity Score (CT-SS) differs between the two groups; not vaccinated patients had a more severe CT imaging than the vaccinated (< 0.00005). PE prevalence in ICU was 43.2% (16/37 patients), while in the Internal Medicine ward, it was 12% (3/25 cases). PE was significantly higher among unvaccinated people: 16/35 (45.7%) vs 3/27 (11.1%), OR p = 0.04. We observed a strong association between vaccination and protection from PE in hospitalized COVID-19 patients: morbidity was significantly lower in vaccinated versus not vaccinated patients. The issue of the protective role of vaccination in COVID-19-associated VTE should be addressed in adequately designed and powered future prospective studies.
A mixed strength decomposition method for identifying critical nodes by decomposing weighted social networks
Authors: Yin Hang , Hou Jishan , Gong Chengju 
Published: May 2023

Abstract
Identifying critical nodes is an efficient strategy for preventing the dynamics of risk dissemination. The properties of edges connecting to the removed nodes are assumed to be the same by many decomposition methods. However, the edge weights are always different in weighted social networks since they have certain practical implications. In this study, a mixed strength decomposition (MSD) method is proposed to identify critical nodes in weighted social networks. This method aims to address the issue of not accounting for the information on removed nodes by considering both residual strength and exhausted strength. Three experimental analyses —the monotonicity test, Susceptible-Infected (SI) diffusion simulation, and successive node removal experiments— conducted on six real-world networks demonstrate that the MSD method has a competitive performance in identifying critical nodes, which overcomes the instability of the node strength and the degeneracy of the s-core method.
Lyric Dispossession as Solitary Presence
Authors: Galvez Marisa 
Published: May 2023

Abstract
While medieval literature offers many models of solitary thinking, vernacular lyric confronts the problem of solitude in a unique mode, grappling and coping with this phenomenon that gives shape and texture to ambivalence and vexation. Comparing the event of lyric dispossession with Petrarch’s idea of solitude, this essay examines solitary presence as a musicopoetic art form across various vernacular traditions, from the Occitan works of Bernart de Ventadorn, William IX, and Arnaut Daniel to lyrics of the Iberian Peninsula, including the Mozarabic kharja (final stanzas of poems written in Arabic or Hebrew in the Romance dialect of Andalusia) and a Galician-Portuguese cantiga d’amigo (songs in which a young girl laments the absence of her lover). Lyric dispossession can affirm female desire despite its dominance as a male solitary presence in the courtly tradition. In the poetic dynamics of the muwashshah, discourses of dispossession compete through the interaction of different languages and social registers. The muwashshah poetics illuminates how the female-voiced solitary presence is maintained not only in the cantiga d’amigo but also in other genres such as trobairitz cansos and Old French crusade departure lyrics from the perspective of women left behind.