bluuebunny/crossref_metadata_2025_split huggingface dataset:
A Rare Case of Prurigo Pigmentosa in Iranian Sibling Couple
Authors: Al‐Zahawi Saman , Saberi Fatemeh , Azhari Vahidesadat , Kamyab Kambiz , Balighi Kamran 
Published: January 2025

ABSTRACTPrurigo Pigmentosa is a rare inflammatory skin disease of unknown origin, characterized by pruritic, erythematous papules on the chest, back, neck, and anterior abdomen. The eruption resolves with reticular hyperpigmentation that cosmetically affects the patient's quality of life. Previous reports highlighted the role of the Ketogenic diet in triggering the disease in young female patients, however, no study reported the occurrence of Prurigo Pigmentosa in siblings of one family, unrelated to a ketogenic diet. Here, we report two sisters diagnosed with Prurigo Pigmentosa without undergoing a Ketogenic diet.
Combination of Bio‐ and Organometallic Catalysis for the Synthesis of Dioxolanes in Organic Solvents
Authors: Nicolas Maria , Gaelings Niklas , Wiesenthal Jan , von Westarp William Graf , Pehlivanlar Benjamin , Pischinger Stefan , Jupke Andreas , Klankermayer Jürgen , Rother Dörte 
Published: January 2025

AbstractIn the catalytic conversion of renewable raw materials, it is essential to adapt the reaction media to match the complexity of substrates. Recently, integrated bio‐ and organometallic catalysis processes have emerged; however, only a few operate in purely organic solvents, which would be advantageous for more energy‐efficient processes. In this study, we present a process using one enzymatic step and two organometallic steps to produce the cyclic acetals 4,5‐dipropyl‐1,3‐dioxolane and 4,5‐dibutyl‐1,3‐dioxolane in a single organic solvent. The enzymatic step, in which a lyase is used, starts from the aldehyde, butanal orpentanal, and forms the 2‐hydroxy ketones butyroin or valeroin, respectively. Subsequently, two organometallic steps were carried out sequentially in one reaction vessel. In a first step, the 2‐hydroxy ketones are hydrogenated to 4,5‐octanediol and 5,6‐decanediol and in the second step the dioxolanes are formed by using hydrogen and carbon dioxide. Either formic acid or polyoxymethylene was used as an alternative carbon source to CO2, which allowed considerable raw material flexibility. Since these dioxolanes are being investigated as additives in biofuel blends, the derived cetane number of the synthesized compounds was measured in addition to the viscosity and density. The cetane numbers determined suggest that the produced dioxolanes could be used as additives in fuel blends.
Microbubble Plasma Processing for N‐Fertigation via Plasma Catalysis
Authors: Zhuang Changping , Long Nguyen Van Duc , Tran Nam Nghiep , Zhang Tianqi , Cullen Patrick , Akay Galip , Hessel Volker 
Published: January 2025

AbstractPlasma can use intermittent renewable energy and abundant use of air to produce N‐fertilizers, with option of decentralized production “at‐farm”. Plasma can generate both nitrate and ammonia (NH3) as key platform molecules toward all N‐fertilizers, including urea and ammonium nitrate. The most convenient way is to produce a “performance chemical” at the farm site. This is a fertigation solution that is ready to be pumped to the farm fields. This study reports about using a nonthermal plasma to generate nitrate and NH3 in presence of water, in which these are immediately absorbed; creating a fertigation solution, termed in literature “plasma activated water (PAW)”. Both air and nitrogen plasmas have been used. The N‐content has been optimized by a process‐parametric study, yielding the second‐best result reported in literature and the best when concerning scale‐up ability. Strategic catalyst studies have been conducted, in terms of plasma‐catalysis synergy, varying the type of catalyst and the location of catalyst. Silica‐supported single/binary metal catalysts and γ‐alumina powder catalysts were used. Distinct catalyst placements inside the plasma electrode zone (glow or spark) and outside in the gas–liquid reactor were tested; with the aid of supports and without. We used proprietary NH3 and N‐fixation catalysts besides specific commercial catalysts. Increasing the N‐fixation performance by about 70% as compared to noncatalytic processing, the study used a recycling loop to further boost the N‐fertilizer concentration. A plasma‐catalyst synergy was determined by structural changes of the catalyst transiently (during plasma operation). This laboratory study achieved a result that has potential for commercial N‐fertigation at farm, when multiplied by a factor of 10 via further process intensification, a factor of 10 by increase of plasma reactor scale, and a factor of 10 by reactor parallelization (“numbering‐up”). Admittedly, this is ambitious and needs considerable process development, yet is a possible way to go without a road blocker.
A Hybrid BN‐Doped Nanographene with Narrow Emission Bandwidths for OLEDs
Authors: Diev Vyacheslav V. , Zou Yunlong , Kondakov Denis , Yap Glenn P. A. 
Published: January 2025

AbstractWe describe synthesis of BN‐doped nanographene containing five phenylene units, boron and nitrogen atoms with alternating ortho‐disposition, as well as direct B−N connections. Resulting BN doped nanographene exhibits blue fluorescence at 441 nm with an extraordinarily narrow fluorescence peak with a full width at half maximum (FWHM)=10–11 nm. Crystallography reveals supramolecular organization of this compound in the crystal phase. Initial organic light emitting device (OLED) data suggest that the presence of a directly connected B−N isostere can lead to devices with sufficiently long lifetime as well as narrow emission electro‐luminescence peaks necessary for OLED applications.
Selective and Divergent Synthesis of Naphthalene‐ and Phenanthrene‐Fused Azahelicenes by Turning Rearrangement On or Off
Authors: Maeda Chihiro , Michishita Sayaka , Ema Tadashi 
Published: January 2025

AbstractThe Scholl reaction has been used to synthesize a variety of polycyclic aromatic hydrocarbons, where 1,2‐aryl shifts have sometimes occurred to yield unique rearrangement products. However, such 1,2‐aryl shifts are often uncontrollable, and the selective and divergent synthesis with or without rearrangement is desired. Here, we achieved the control of the rearrangement in the Scholl reaction of carbazoles by changing the N‐substituents. The Scholl reaction of 3,6‐bis{2‐(2‐naphthyl)phenyl}carbazoles and 3,6‐bis{2‐(9‐phenanthrenyl)phenyl}carbazoles with an N‐benzyl group gave multiple azahelicenes via double rearrangement, while those with an N‐benzoyl group gave aza[9]helicene and quadruple [4]helicene in the former and latter cases, respectively. The reaction mechanisms on the divergent reaction pathways were investigated by DFT calculations, which well supported the experimental results.
Frustration Intolerance Scale for Students
Authors: Simona Trip , Hortensia Bora Carmen , Gabriel Roseanu 
Published: January 2025

ABSTRACTBackgroundFrustration intolerance (FI) is the belief that reality should meet our desires for ease, pleasure and comfort. Although extensively studied in adults, tools for measuring FI in children and adolescents are scarce. The Frustration Intolerance Scale for Students (FISS) was developed to fill this gap. This study aimed to evaluate the FISS's structure and, through a longitudinal design, assess its psychometric properties, including reliability and validity.MethodsConfirmatory factor analysis (CFA) was conducted with a sample of 780 participants (46.30% female, Agemean = 11.80 years). A retest was administered after 5 months to 611 participants (48.6% female, Agemean = 12.24 years). Both testing moments assessed FI, along with externalized and internalized anger, anger duration, empathy, academic interest and self‐efficacy.ResultsConfirmatory factor analyses revealed that the eight‐plus‐one model is the optimal structure for the FISS, allowing for scores on each FI component, frustration tolerance (FT) and a general irrationality FI factor. The eight subscales and the irrationality FI factor showed good internal consistency, whereas test–retest reliability was fair, indicating some variability between administrations. Regarding convergent evidence, significant longitudinal correlations were found between internalized and externalized anger, the degree of anger and all dimensions of FI. FT was positively associated with empathy, and this pattern remained consistent over time. The scale demonstrated discriminant validity by effectively differentiating between students with low and high levels of academic interest and self‐efficacy.ConclusionsThe FISS is a valid and reliable tool for assessing various dimensions of frustration intolerance in children and adolescents.
Increased Phosphorylation of Intracellular Signaling Molecules Indicates Continuous Activation of Human Autoreactive B‐Cells
Authors: Kroos Sanne , Blomberg Nienke J. , Kwekkeboom Joanneke C. , Hendriks Rudi W. , Corneth Odilia B. J. , Toes René E. M. , Scherer Hans U. 
Published: January 2025

ABSTRACTMany human autoimmune diseases (AIDs) are hallmarked by the presence and persistence of autoreactive B‐cells. While autoreactive B‐cells may frequently encounter antigens, the signals required to balance and maintain their activation and survival are mostly unknown. Understanding such signals may be important for strategies aimed at eliminating human B‐cell autoreactivity. Here, we assessed intracellular signaling pathways in B cells targeting citrullinated protein antigens isolated from patients with rheumatoid arthritis (RA), a common and well‐characterized AID. Peripheral blood mononuclear cells of 15 RA patients positive for anti‐citrullinated protein antibodies (ACPA) were analyzed directly ex vivo using spectral flow cytometry and B‐cell differentiation markers, citrullinated antigen‐biotin‐streptavidin tetramers, and intracellular (phosphoflow) markers. Tetanus toxoid (TT)‐specific B cells served as antigen‐specific comparators. In absence of any in vitro BCR stimulation, ACPA‐expressing memory B cells (MBCs) displayed enhanced expression of Ki‐67 and increased SYK‐, BTK‐, AKT‐, and S6‐phosphorylation compared with TT‐specific MBCs. We demonstrate the simultaneous detection of B cell antigen‐specificity and intracellular protein phosphorylation on the single‐cell level. The data reveal that autoreactive B‐cells in RA, in contrast to B cells against recall antigens, display enhanced phosphorylation of signaling molecules that point toward continuous, presumably antigen‐mediated activation of the autoreactive B‐cell compartment.
Leaders or Villains? The Role of Corruption in Shaping the Stereotypes of Politicians
Authors: Ascenso Inês , Ramos Miguel R. , Moriconi Marcelo , Marques Sibila 
Published: January 2025

ABSTRACTThe ways in which politicians are stereotyped contribute to perceptions of the legitimacy of their power, status and leadership. In this research, we examined how corruption impacts the stereotypes of politicians. By examining stereotype content, we posited that corruption should elicit morality‐related traits, while in contexts of no corruption, both morality and competence should emerge as key dimensions. With two experiments (Ncombined = 272), participants were presented with either a corruption or no corruption condition and were asked to provide traits describing politicians. Results revealed that in the corruption conditions, (low) morality emerged as the key dimension explaining most variance. In the no‐corruption conditions, (high) morality and (high) competence emerged as a single key dimension. Our findings reveal one of the multiple pathways by which corruption impacts society–perceiving politicians as having extremely low morality has implications for general trust and meritocracy, contributing to the erosion of democracy.
Unlocking the Healing Potential: A Comprehensive Review of Ecology and Biology of Medical‐Grade Honey in Wound Management and Tissue Regeneration
Authors: Barazesh Parmis , Hajihassani Helia , Motalebi Fatemeh , Neiresi Seyedeh Mobina Hosseini , Hajihassani Romina , Mehrabian Ahmad Reza 
Published: January 2025

ABSTRACTBackground and AimsHoney has long been studied for its healing abilities in wound care. This narrative review examines its properties and their impact on wound healing, particularly its ability to accelerate wound closure and promote tissue regeneration. The review focuses on how honey's botanical origins affect its medical properties and wound‐healing capabilities. Finally, clinical studies on honey's effectiveness in wound healing were reviewed compared to traditional treatments.MethodsRelevant keywords were searched in databases, yielding 1250 documents. After excluding nonrelevant sources, 450 documents were refined, and 167 articles were selected based on thematic alignment and originality. Data extraction focused on study design, intervention details, and outcomes, with quality assessed using standardized criteria. The study adhered to CONSORT and SANRA guidelines to ensure methodological rigor and reporting transparency.ResultsHoney‐based medical products have demonstrated significant antibacterial, anti‐inflammatory, and tissue‐regenerative properties, making them highly effective in improving wound healing outcomes, particularly in chronic and burn wounds. These products have also been shown to reduce infection rates and hospital stays. While some studies have reported positive outcomes in accelerating the healing process, others have found no significant difference compared to conventional treatments.ConclusionMedical‐grade honey (MGH) holds potential for wound care due to its versatility, though variations in its composition present challenges. Further research is needed to optimize its clinical use. The effectiveness of MGH in wound healing remains debated, with mixed results from trials. Genetic modification of bees to enhance MGH's properties could make it more competitive against conventional treatments. Honey‐based medications could reduce costs, improve energy efficiency, and have minimal side effects. Rigorous research is necessary to determine optimal use and fully unlock MGH's potential, which could revolutionize wound management globally.
Integrating pharmacogenetics, multi‐omics and machine learning in the novel therapeutic view of pulmonary hypertension
Authors: Li Chenxi , Xue Jipeng , Zhou Dongchen , Qi Xiaomeng , Chen Ting , Li Zhoubin 
Published: January 2025

AbstractIt is a complex disease with multiple pathogenesis that causes pulmonary hypertension (PH). In addition to the commonly used drugs for treating PH, pharmacogenomic therapies are facilitating the innovation of personalized medicine to treat PH. Meanwhile, gene therapy has been extensively studied but is a quite challenging task. Furthermore, the gradual widespread application of omics analyses, big data and machine learning is a trend to improve the efficacy of drugs and gene therapy of PH. They are widely used in the screening of drug therapeutic targets and predicting drugs therapeutic effect. The emergence of innovative diagnosis and treatment approaches for PH opens pathways for combining personalized medicine with established pharmacological and gene therapies to achieve effective treatment strategies and further decrease the mortality rate associated with this disease.
Development of an easy non‐destructive particle isolation protocol for quality control of red blood cell concentrates
Authors: Ghodsi Marine , Cloos Anne‐Sophie , Lotens Anaïs , De Bueger Marine , Van Der Smissen Patrick , Henriet Patrick , Cellier Nicolas , Pierreux Christophe E. , Najdovski Tomé , Tyteca Donatienne 
Published: January 2025

AbstractThe extracellular vesicle release in red blood cell concentrates reflects progressive accumulation of storage lesions and could represent a new measure to be implemented routinely in blood centres in addition to haemolysis. Nevertheless, there is currently no standardized isolation protocol. In a previous publication, we developed a reproducible ultracentrifugation‐based protocol (20,000 × g protocol) that allows to classify red blood cell concentrates into three cohorts according to their vesiculation level. Since this protocol was not adapted to meet routine requirements, the goal of this study was to develop an easier method based on low‐speed centrifugation (2,000 × g protocol) and limited red blood cell concentrate volumes to match with a non‐destructive sampling from the quality control sampling tubing. Despite the presence of contaminants, mainly in the form of albumin and lipoproteins, the material isolated with the 2,000 × g protocol contained red blood cell‐derived vesicular structures. It was reproducible, could predict the number of extracellular vesicles obtained with the 20,000 × g protocol and better discriminated between the three vesiculation cohorts than haemolysis at the legal expiry date of 6 weeks. However, by decreasing red blood cell concentrate volumes to fit with the volume in the quality control tubing, particle yield was highly reduced. Therefore, centrifugation time and relative centrifugal force were adapted (1,000 × g protocol), allowing for the recovery of a similar particle number and composition between small and large volumes sampled from the main unit, in different vesiculation cohorts over time. A similar observation was made with the 1,000 × g protocol between small volumes sampled from the quality control tubing and the mother‐bag. In conclusion, our study paves the way for the use of the 2,000 × g protocol (adapted to a 1,000 × g protocol with the quality control sampling tubing) for particle measurement in blood centres.
D,L‐3‐hydroxybutyrate in the treatment of glucose transporter 1 deficiency syndrome (Glut1DS)
Authors: Amer Aya , Murrell Kathryn , Edmonds Liza , Bernhardt Isaac , Akroyd Rhonda , Ryder Bryony , Wilson Callum , Glamuzina Emma 
Published: January 2025

AbstractBackgroundDeficiency of the Glut1 transporter due to mono‐allelic variants in SLC2A1 causes hypoglycorrhachia, resulting in a neurological spectrum from neonatal epilepsy to adult‐onset paroxysmal movement disorders (PMD). The brain utilises ketone bodies as an alternative energy source to glucose. Thus, early initiation of the ketogenic diet (KD) is standard care for Glut1 deficiency syndrome (Glut1DS). Commencement and adherence in older Glut1DS patients is difficult to achieve, leaving few treatment options. Oral D,L‐3‐hydroxybutyrate (D,L‐3‐HB) crosses the blood–brain barrier, making it a potential treatment for Glut1DS.MethodsA retrospective case review of patients with Glut1DS under the Adult and Paediatric National Metabolic Service (APNMS) of New Zealand, treated with D,L‐3‐HB between 2012 and 2023 was performed. Clinical notes, standardised, neuropsychological assessments and subjective data on and off D,L‐3‐HB were obtained. The best on and off D,L‐3‐HB measures of working memory (WMI) and processing speed (PSI) were compared to assess the efficacy.ResultsD,L‐3‐HB was offered to 12 patients with Glut1DS (age 10–52 years). Compliance‐dependent improvements in subjective, cognitive and adaptive function were reported by those who were reassessed on‐treatment (9/12). Four reported improved PMD. Objective improvements were found in WM (9/9) and PS (6/9). Subjective improvements were reported in patients' health, wellbeing and independence.ConclusionsKD remains standard of care for Glut1DS, but effective alternatives are lacking for those who do not tolerate this. D,L‐3‐HB was associated with improved WM, PS and perceived life quality in this small group of patients with Glut1DS, thus providing a potential treatment for this distinct group.
USP7 Inhibitors Destabilize EBNA1 and Suppress Epstein‐Barr Virus Tumorigenesis
Authors: Chen Christopher , Addepalli Kush , Soldan Samantha S. , Castro‐ Munoz Leonardo Josue , Preston‐Alp Sarah , Patel Rishi J. , Albitz Coltin J. , Tang Hsin‐Yao , Tempera Italo , Lieberman Paul M. 
Published: January 2025

ABSTRACTEpstein‐Barr virus (EBV) is a ubiquitous human ɣ‐herpesvirus implicated in various malignancies, including Burkitt's lymphoma and gastric carcinomas. In most EBV‐associated cancers, the viral genome is maintained as an extrachromosomal episome by the EBV nuclear antigen‐1 (EBNA1). EBNA1 is considered to be a highly stable protein that interacts with the ubiquitin‐specific protease 7 (USP7). Here, we show that pharmacological inhibitors and small interfering RNA (siRNA) targeting USP7 reduce EBNA1 protein levels in a proteosome‐dependent manner. Proteomic analysis revealed that USP7 inhibitor GNE6776 altered the EBNA1 protein interactome, including disrupting USP7 association with EBNA1. GNE6776 also inhibited EBNA1 binding to EBV oriP DNA and reduced viral episome copy number. Transcriptomic studies revealed that USP7 inhibition affected chromosome segregation and mitotic cell division pathways in EBV+ cells. Finally, we show that GNE6776 selectively inhibited EBV+ gastric and lymphoid cell proliferation in cell culture and slowed EBV+ tumor growth in mouse xenograft models. These findings suggest that USP7 inhibitors perturb EBNA1 stability and function and may be exploited to treat EBV latent infection and tumorigenesis.
Evaluating the influence of snow and ice conditions on under‐ice light regimes, dissolved oxygen, and primary production in shallow lakes using controlled manipulative systems
Authors: Barrett David C. , Wrona Frederick J. 
Published: January 2025

AbstractShifts in hydroclimatic regimes associated with global climate change are affecting the timing and duration of winter ice‐cover in temperate and high‐latitude lakes. Less is known on how associated changes in ice quality potentially affect under‐ice ecological processes and related biogeochemistry, particularly in shallow lake systems. This experimental study examined how differing surface ice‐cover types (slushed white ice, unmanipulated ice formation [black], snow‐on‐ice) affected under‐ice light and oxygen regimes and associated chlorophyll a concentrations, and water quality parameters using a combination of aboveground mesocosms and natural ponds over two consecutive winter seasons. In both systems, light transmission under white ice did not differ significantly from black but was significantly greater than snow‐on‐ice. Measures of light extinction coefficients taken at the ice‐water interface in the experimental ponds increased for black, white, and snow‐on‐ice, respectively, with all values being above thresholds for autotrophic activity. Under‐ice dissolved oxygen levels in all mesocosm ice treatments increased continually over time with increasing day length, peaking prior to ice off. Accumulated DO levels were aligned with observed chlorophyll a concentrations, with peaks early after ice onset, and decreases as winter progressed though patterns differed between years. No significant differences were observed in mean chlorophyll a levels in snow‐removed treatment ponds, regardless of whether white or black ice was present while the snow‐on‐ice ponds displayed consistently lower levels. This study highlights the complexities and importance of using manipulative experimental approaches to advance our understanding of how alterations in surface ice‐cover quality affect under‐ice hydro‐ecological processes.