bluuebunny/crossref_metadata_2025_split huggingface dataset:
RNA-binding protein CCDC137 activates AKT signaling and promotes hepatocellular carcinoma through a novel non-canonical role of DGCR8 in mRNA localization
Authors: Tao Shuang , Xie Shu-Juan , Diao Li-Ting , Lv Guo , Hou Ya-Rui , Hu Yan-Xia , Xu Wan-Yi , Du Bin , Xiao Zhen-Dong 
Published: August 2023

Abstract
Background
RNA binding proteins (RBPs)—regulated gene expression play a vital role in various pathological processes, including the progression of cancer. However, the role of RBP in hepatocellular carcinoma (HCC) remains much unknown. In this study, we aimed to explore the contribution of RBP CCDC137 in HCC development.

Methods
We analyzed the altered expression level and clinical significance of CCDC137 in database and HCC specimens. In vitro cell assays and in vivo spontaneous mouse models were used to assess the function of CCDC137. Finally, the molecular mechanisms of how CCDC137 regulates gene expression and promotes HCC was explored.

Results
CCDC137 is aberrantly upregulated in HCC and correlates with poor clinical outcomes in HCC patients. CCDC137 markedly promoted HCC proliferation and progression in vitro and in vivo. Mechanistically, CCDC137 binds with FOXM1, JTV1, LASP1 and FLOT2 mRNAs, which was revealed by APOBEC1-mediated profiling, to increase their cytoplasmic localization and thus enhance their protein expressions. Upregulation of FOXM1, JTV1, LASP1 and FLOT2 subsequently synergistically activate AKT signaling and promote HCC. Interestingly, we found that CCDC137 binds with the microprocessor protein DGCR8 and DGCR8 has a novel non-canonical function in mRNA subcellular localization, which mediates the cytoplasmic distribution of mRNAs regulated by CCDC137.

Conclusions
Our results identify a critical proliferation-related role of CCDC137 and reveal a novel CCDC137/DGCR8/mRNA localization/AKT axis in HCC progression, which provide a potential target for HCC therapy.
Novel corticotropin-releasing hormone receptor genes (CRHR1 and CRHR2) linkage to and association with polycystic ovary syndrome
Authors: Amin Mutaz , Horst Nicholas , Wu Rongling , Gragnoli Claudia 
Published: August 2023

Abstract
Background
Women with polycystic ovarian syndrome (PCOS) have increased hypothalamic–pituitary–adrenal (HPA) axis activation, pro-inflammatory mediators, and psychological distress in response to stressors. In women with PCOS, the corticotropin-releasing hormone (CRH) induces an exaggerated HPA response, possibly mediated by one of the CRH receptors (CRHR1 or CRHR2). Both CRHR1 and CRHR2 are implicated in insulin secretion, and variants in CRHR1 and CRHR2 genes may predispose to the mental-metabolic risk for PCOS.

Methods
We phenotyped 212 Italian families with type 2 diabetes (T2D) for PCOS following the Rotterdam diagnostic criteria. We analyzed within CRHR1 and CRHR2 genes, respectively, 36 and 18 microarray-variants for parametric linkage to and/or linkage disequilibrium (LD) with PCOS under the recessive with complete penetrance (R1) and dominant with complete penetrance (D1) models. Subsequentially, we ran a secondary analysis under the models dominant with incomplete penetrance (D2) and recessive with incomplete penetrance (R2).

Results
We detected 22 variants in CRHR1 and 1 variant in CRHR2 significantly (p < 0.05) linked to or in LD with PCOS across different inheritance models.

Conclusions
This is the first study to report CRHR1 and CRHR2 as novel risk genes in PCOS. In silico analysis predicted that the detected CRHR1 and CRHR2 risk variants promote negative chromatin activation of their related genes in the ovaries, potentially affecting the female cycle and ovulation. However, CRHR1- and CRHR2-risk variants might also lead to hypercortisolism and confer mental-metabolic pleiotropic effects. Functional studies are needed to confirm the pathogenicity of genes and related variants.
Large-scale reference-free analysis of flavivirus sequences in Aedes aegypti whole genome DNA sequencing data
Authors: Spadar Anton , Phelan Jody E. , Clark Taane G. , Campino Susana 
Published: August 2023

AbstractFlaviviruses are a diverse group of RNA viruses, which include the etiological agents of Zika, dengue and yellow fever that are transmitted by mosquitoes. Flaviviruses do not encode reverse transcriptase and cannot reverse transcribe into DNA, yet DNA sequences of flaviviruses are found both integrated in the chromosomes of Aedes aegypti mosquitoes and as extrachromosomal sequences. We have previously examined the Ae. aegypti reference genome to identify flavivirus integrations and analyzed conservation of these sequences among whole-genome data of 464 Ae. aegypti collected across 10 countries globally. Here, we extended this analysis by identifying flavivirus sequences in these samples independently of the Ae. aegypti reference assembly. Our aim was to identify the complete set of viral sequences, including those absent in the reference genome, and their geographical distribution. We compared the identified sequences using BLASTn and applied machine learning methods to identify clusters of similar sequences. Apart from clusters of sequences that correspond to the four viral integration events that we had previously described, we identified 19 smaller clusters. The only cluster with a strong geographic association consisted of Cell-fusing agent virus-like sequences specific to Thailand. The remaining clusters did not have a geographic association and mostly consisted of near identical short sequences without strong similarity to any known flaviviral genomes. The short read sequencing data did not permit us to determine whether identified sequences were extrachromosomal or integrated into Ae. aegypti chromosomes. Our results suggest that Liverpool strain and field Ae. aegypti mosquitoes have a similar variety of conserved flaviviral DNA, whose functional role should be investigated in follow-up studies.
Graphical Abstract
Post-infectious inflammatory response syndrome in an HIV-negative patient after Cryptococcus gattii meningoencephalitis: a case report and review of the literature
Authors: Lan Jianhua , Lv Luyi , Ye Ling , Wang Tao , Wu Zhiyu , Wu Shugen , Peng Chunxian , Lu Weili , Lu Tao 
Published: August 2023

Abstract
Background
Cryptococcal meningitis (CM) is an inflammatory mycosis of the central nervous system caused by meninge infection or brain parenchyma with Cryptococcus species. It is associated with high morbidity and mortality, and patients with acquired immune deficiency syndrome are particularly susceptible. There have been increasing reports of CM in HIV-negative patients in China over the last few years.

Case presentation
A 31-year-old healthy Chinese male presented with fever and gradually developed headache, projectile vomiting, and other manifestations that were later confirmed as Cryptococcus gattii meningoencephalitis. However, multiple disease changes occurred during the course of treatment, and the regimen was accordingly modified after the diagnosis of post-infectious inflammatory response syndrome (PIIRS). The patient eventually recovered.

Conclusion
There has been a growing trend in the incidence of C. gattii meningoencephalitis in HIV-negative patients. It shows rapid onset and severe prognosis. This case report can provide a reference to treat PIIRS following CM in HIV-negative patients.
Regulation of serotonin production by specific microbes from piglet gut
Authors: Liu Ziyu , Ling Yidan , Peng Yu , Han Shuibing , Ren Yuting , Jing Yujia , Fan Wenlu , Su Yong , Mu Chunlong , Zhu Weiyun 
Published: August 2023

Abstract
Background
Serotonin is an important signaling molecule that regulates secretory and sensory functions in the gut. Gut microbiota has been demonstrated to affect serotonin synthesis in rodent models. However, how gut microbes regulate intestinal serotonin production in piglets remains vague. To investigate the relationship between microbiota and serotonin specifically in the colon, microbial composition and serotonin concentration were analyzed in ileum-cannulated piglets subjected to antibiotic infusion from the ileum when comparing with saline infusion. Microbes that correlated positively with serotonin production were isolated from piglet colon and were further used to investigate the regulation mechanisms on serotonin production in IPEC-J2 and a putative enterochromaffin cell line RIN-14B cells.

Results
Antibiotic infusion increased quantities of Lactobacillus amylovorus (LA) that positively correlated with increased serotonin concentrations in the colon, while no effects observed for Limosilactobacillus reuteri (LR). To understand how microbes regulate serotonin, representative strains of LA, LR, and Streptococcus alactolyticus (SA, enriched in feces from prior observation) were selected for cell culture studies. Compared to the control group, LA, LR and SA supernatants significantly up-regulated tryptophan hydroxylase 1 (TPH1) expression and promoted serotonin production in IPEC-J2 cells, while in RIN-14B cells only LA exerted similar action. To investigate potential mechanisms mediated by microbe-derived molecules, microbial metabolites including lactate, acetate, glutamine, and γ-aminobutyric acid were selected for cell treatment based on computational and metabolite profiling in bacterial supernatant. Among these metabolites, acetate upregulated the expression of free fatty acid receptor 3 and TPH1 while downregulated indoleamine 2,3-dioxygenase 1. Similar effects were also recapitulated when treating the cells with AR420626, an agonist targeting free fatty acid receptor 3.

Conclusions
Overall, these results suggest that Lactobacillus amylovorus showed a positive correlation with serotonin production in the pig gut and exhibited a remarkable ability to regulate serotonin production in cell cultures. These findings provide evidence that microbial metabolites mediate the dialogue between microbes and host, which reveals a potential approach using microbial manipulation to regulate intestinal serotonin biosynthesis.
Adapting family-based treatment for adolescent anorexia nervosa delivered in the home: A novel approach for improving access to care and generalizability of skill acquisition
Authors: Goldschmidt Andrea B. , Tortolani Christina C. , Accurso Erin C. , Dunbar Eva-Molly Petitto , Egbert Amy H. , Donaldson Deidre , Donaldson Abigail A. 
Published: August 2023

Abstract
Background
Anorexia nervosa (AN) is a serious mental illness associated with high rates of morbidity and mortality. Family-based treatment (FBT) is a well-established treatment for adolescent AN, yet it is underutilized in community settings and is unavailable to many families, particularly those from lower income and racial and ethnic minority backgrounds. Furthermore, some families do not respond optimally to FBT, possibly because of challenges translating skills acquired in office-based treatment settings to naturalistic settings. Home-based treatment could reduce barriers to access and enhance generalization of newly learned treatment skills. Home-based models demonstrate initial feasibility, acceptability, and efficacy for adolescent AN, however, FBT principles have yet to be applied as a stand-alone intervention in a home-based level of care. This paper describes the rationale for and process of adapting FBT principles/interventions to improve fit within a home-based model delivered in the context of community mental health, and discusses potential strengths and opportunities associated with this approach.

Results
Adaptations were made through consultation with collaborating community agencies and were guided by the complex interventions framework. The primary modifications included: (1) altered dose; (2) multiple family meals; (3) additional support for meal preparation and supervision; (4) clinician attendance at medical appointments; (5) cultural adaptation; and (6) introduction of distress tolerance and emotion regulation skills.

Conclusions
Implementing FBT in the home may present one promising and novel approach to enhance engagement and treatment outcomes for adolescents with restrictive eating disorders, particularly those who are underserved, but evaluation of efficacy/effectiveness is needed.
Caregiver and Juvenile Justice Personnel Perspectives on challenges and importance of caregiver engagement and the potential utility of a peer navigator program in the Juvenile Justice System
Authors: Dir Allyson L. , Pederson Casey , Khazvand Shirin , Schwartz Katie , Wiehe Sarah E. , Aalsma Matthew C. 
Published: August 2023

Abstract
Background
For youth involved in the juvenile justice (JJ) system, caregiver involvement and engagement in the system is crucial for youth development and outcomes of JJ cases; however, there are challenges to establishing positive/productive partnerships between caregivers and JJ representatives. The current project examines perspectives of caregivers and JJ personnel regarding facilitators and barriers to establishing JJ-caregiver partnerships, as well as their perceptions of the use of a caregiver navigator program to support caregivers of system-involved youth. Results are used to inform development of a caregiver navigator program to support caregivers and help them navigate the JJ system.

Results
Semi-structured interviews were conducted with caregivers of youth involved in JJ (n = 15, 53% White, 93% female), JJ personnel (n = 7, 100% White, 50% female), and JJ family advisory board members (n = 5, 100% Black, 100% female). Caregivers reported varying experiences across intake/arrest, court, and probation processes. Positive experiences were characterized by effective communication and feeling supported by JJ. Negative experiences related to feeling blamed and punished for their child’s system involvement and feeling unsupported. JJ interviews corroborated caregiver sentiments and also illustrated facilitators and barriers to JJ-caregiver partnerships. Both JJ personnel and caregivers endorsed potential benefits of a peer-based caregiver navigator program to provide social, informational, and emotional support.

Conclusion
Continued work is needed to improve JJ-caregiver partnerships and use of a peer-based navigator program has the potential to address barriers to caregiver engagement in the JJ system.
Preservation conditions and potential evaluation of the Longmaxi shale gas reservoir in the Changning area, southern Sichuan Basin
Authors: Xie Weidong , Chen Si , Gan Huajun , Wang Hua , Wang Meng , Vandeginste Veerle 
Published: August 2023

AbstractThe production of shale gas varies greatly in different regions due to the way gas has accumulated and preserved. This work investigates the dynamic evolution of shale gas generation, accumulation, adjustment, and loss from the Longmaxi formation (S1l) in the Changning area, southern Sichuan Basin, China. The factors controlling the preservation conditions and formation mechanism of the overpressure shale gas reservoir are also studied. The results show that shale gas generation reached its peak during the Middle Jurassic to Early Cretaceous. Furthermore, the gas occurs mainly in organic matter pores of nanometer size, clay mineral pores of nano- to micro-meter size, and microfractures of micrometer size. Then, in the Early Cretaceous, the reservoir was damaged due to uplift of the crust. Additionally, the evaluation scheme of the shale gas reservoirs is established according to the organic geochemical parameters, mineralogical composition, sealing capacity, thickness, burial depth, faults, pressure coefficient, and gas content, etc. Hence, the shale gas reservoirs may be divided into four grades, with Class I being the grade with best gas preservation and Non-economic grade with the worst gas preservation. The annular region in the Jianwu–Luochang synclines and the northeast limb of the Changning anticline have optimum preservation conditions, with a grade of Class I. The preservation conditions gradually deteriorate towards the two limbs, with Class II, Class III, and Non-economic area grades. The good preservation conditions correspond to a high pressure coefficient, and the pressure of the reservoir is mainly caused by hydrocarbon generation pressurization of organic matter (mainly the stage of oil cracking gas and dry gas), tectonic uplift pressurization, and to a minor extent, transformation dehydration pressurization of clay minerals. Furthermore, overpressure preservation is controlled by microporous overpressure, source rock–caprock vertical sealing ability, the spatial distribution of S1l, and development characteristics of faults. Results from this investigation provide specific guidance for shale gas exploitation in the study area, and provide a reference for the evaluation of preservation conditions in shale gas reservoirs and formation mechanism of overpressure gas reservoirs.
Screening and management of dyslipidemia in oncologic patients undergoing cardiotoxic therapies: results from an Italian survey
Authors: Camilli Massimiliano , Bisceglia Irma , Canale Maria Laura , Turazza Fabio Maria , De Luca Leonardo , Gabrielli Domenico , Gulizia Michele Massimo , Oliva Fabrizio , Colivicchi Furio 
Published: August 2023

Abstract
Background
Baseline cardiovascular risk factors correction is recommended in all cancer patients undergoing potentially cardiotoxic therapies. Despite available guidelines, real-world data on dyslipidemia prevalence and management in the oncologic population are still sparse.

Methods
This survey was an Italian, investigator-initiated survey initially designed and drafted by the Cardio-Oncology section of the Associazione Nazionale Medici Cardiologi Ospedalieri (ANMCO), comprising 10 individual multi-choice questions and spread after validation through the ANMCO mailing list. The survey was sent to cardiologists working in cardio-oncology units and/or managing patients with cancer.

Results
Our survey included 139 Italian cardiologists. The majority of them routinely ask for the baseline lipidic profile of their patients, regardless of previous clinical history and planned treatment. According to our participants, the estimated prevalence of dyslipidemia in this population is between 20% and 60%. Although this high prevalence, our results highlight that there is poor harmony in terms of scores for CV risk prediction used in clinical practice to guide drug prescription and baseline therapy optimization. On the same line, coronary artery calcium score is poorly used in this setting. At the same time, more than 30% of interrogated physicians do not prescribe adequate statin doses, even though necessary, and have uncertainties on the use of other anti-dyslipidemic drugs in this population.

Conclusions
Our results highlight the necessity of strong evidences on dyslipidemia screening and management in the cancer population, as well as the need of knowledge diffusion from scientific societies to clinicians treating these patients.
Relationship between estimated and observed heparin sensitivity indices in cardiac and thoracic aortic surgery
Authors: Nakatani Hitomi , Ida Mitsuru , Kotani Taichi , Kawaguchi Masahiko 
Published: August 2023

Abstract
Background
Little evidence exists on the relationship between the estimated heparin sensitivity index (HSI) based on commonly available clinical and laboratory data and observed HSI in the adult population. This retrospective study assessed the relationship between the observed and estimated HSIs.

Methods
This study was conducted in an academic, single-institution setting. Patients aged ≥ 20 years who underwent cardiac and thoracic aortic surgery and requiring cardiopulmonary bypass were included. Clinical and laboratory data, including age, sex, and platelet count, were collected. The fibrinogen-albumin ratio index was calculated by dividing the fibrinogen value by the albumin value, multiplied by 10.The HSI was calculated using the formula: (activated clotting time after initial heparin administration—baseline activated clotting time)/initial heparin dose (IU/kg). The estimated HSI was based on the results of multiple regression analysis that included clinically relevant factors. The intraclass correlation coefficient between the observed and estimated HSIs was used to assess.

Results
In total, 560 patients with valid activated clotting time (ACT) values after initial heparin administration were included in the final analysis to explore associated factors using the estimated HSI. Multiple regression analysis revealed that hemodialysis, platelet count, fibrinogen-to-albumin ratio index, baseline activated clotting time, and initial heparin dose were significantly associated with the HSI. The mean (standard deviation) observed and estimated HSIs were 1.38 (0.43) and 1.55 (0.13), respectively, with an intra-class correlation coefficient of 0.10.

Conclusions
The correlation between the observed and estimated HSIs was low, and a formula with high accuracy for estimating the HSI is needed.
Characterization of a new isolate of Beauveria bassiana in Algeria and evaluation of its pathogenicity against the cowpea aphid (Aphis craccivora Koch)
Authors: Akrich Amine , Righi Kada , Righi Fatiha Assia , Elouissi Abdelkader 
Published: August 2023

Abstract
Background
The cowpea aphid, Aphis craccivora Koch (Hemiptera: Aphidiidae) is a polyphagous aphid species that causes various damage on different crops. The conventional method of controlling this pest is the use synthetic insecticides that threaten both the environmental safety and human health. Moreover, it contributes to the emergence of insecticide-resistant generations. Hence, relying on Entomopathogenic fungi (EPF) remains one of the most safe and effective alternative solutions to control insect pests. For the mentioned reasons, the EPF, Beauveria bassiana was isolated and characterized; besides, its efficiency against adults’ A. craccivora was evaluated both in the laboratory and in the greenhouse.

Results
A new isolate of B. bassiana was isolated from collected cadavers’ insects associated with the population of A. craccivora in a rural area in the Northwest of Algeria. This isolate was identified on the basis of its morphological and molecular characteristics and was referred to as B. bassiana BBAA. The enzymatic activities of this isolate revealed a high production of chitinase, protease and lipase, without any production of amylase. The use of different concentrations of B. bassiana BBAA conidia against A. craccivora led to a high mortality rate, ranging from 64 to 74% mortality on the seventh day post-treatment in vitro and 58 to 70% in greenhouse.

Conclusion
Virulence and enzymatic activities produced by B. bassiana BBAA demonstrated the necessity to exploit entomopathogenic fungi (EPFs) in pest control.