bluuebunny/crossref_metadata_2025_split huggingface dataset:
Comparative analysis of flower development, active ingredients and transcriptomic response revealed mechanisms of Chrysanthemum morifolium cv. 'Hangju' responding to flooding stress
Authors: Zhang Hong , Yang Feng , Guo Qiaosheng , Yu Shuyan , Pu Jingzhe , Liu Minhua , Zou Qingjun , Zhang Wenyan , Wang Tao 
Published: April 2023

Abstract
C. morifolium cv. 'Hangju' is used as material medicine and herbal tea in China for several centuries. In the previous observation, it was found that sufficient rainfall conditions can affect the flower buds differentiation process of 'Hangju'. In order to study the effects of flooding stress on 'Hangju', this study simulated the flooding stress during the reproductive growth of 'Hangju' and detected the morphological difference of the capitulum and luteoloside content change in different growth stages. RNA-seq was also used to contrast transcriptomes of pre- and post-treatment performance. In total, 101,806 unigenes were obtained and 968, 624, 964, and 582 differentially expressed genes (DEGs) were identified between flooding groups and control groups in the 4 stages. GO enrichment analysis of DEGs was performed to derive the main enrichment entries, KEGG enrichment analysis reveals a significant enrichment of DEGs in the biosynthetic pathway of secondary metabolites, which include flavonoid, flavone, and flavonol biosynthesis pathways. Overall, our research provides reasonable insights into the flower bud differentiation response mechanisms of 'Hangju' is subjected to flooding stress, and will expand our understanding of the transcriptional response to flood stress. These results will provide a better understanding of the formation of quality in 'Hangju' when subjected to flooding stress and will expand our understanding of the transcriptional response to flooding stress. These results will provide greater understanding of the formation of quality in 'Hangju'.
Uterine Artery Embolization in Tanzania: A Procedure with Major Public Health Implications
Authors: Musa Balowa , Alswang Jared Mark , Ioia Rose Di , Grubic Lydia , Naif Azza , Mbuguje Erick Michael , Vuong Victoria , Newsome Janice , Ramalingam Vijay , Gaupp Fabian Max Laage 
Published: April 2023

Abstract
Background The burden of uterine fibroids is substantial in sub-Saharan Africa (SSA), with up to 80% of black women harboring them in their lifetime. While uterine artery embolization (UAE) has emerged as an effective alternative to surgery to manage this condition, the procedure is not available to the vast majority of women living in SSA due to limited access to interventional radiology (IR) in the region. One of the few countries in SSA now offering UAE in a public hospital setting is Tanzania. This study aims to assess the safety and effectiveness of UAE in this new environment.Methods From June 2019 to July 2022, a single-center, retrospective cohort study was conducted at Tanzania’s first IR service on all patients who underwent UAE for the management of symptomatic fibroids or adenomyosis. Patients were selected for the procedure based on symptom severity, imaging findings, and medical management failure. Procedural technical success and adverse events were recorded for all UAEs. Self-reported symptom severity and volumetric response on imaging were compared between baseline and six-months post-procedure using paired sample t-tests.Results During the study period, 92.1% (n = 35/38) of patients underwent UAE for the management of symptomatic fibroids and 7.9% (n = 3/38) for adenomyosis. 97.3% (n = 37/38) were considered technically successful and one minor adverse event occurred (2.7%). Self-reported symptom-severity scores at six-months post-procedure decreased in all categories: abnormal uterine bleeding from 8.8 to 3.1 (-5.7), pain from 6.7 to 3.2 (-3.5), and bulk symptoms from 2.8 to 1 (-1.8) (p < 0.01). 100% of patients reported satisfaction with outcomes. Among the nine patients with follow-up imaging, there was a mean volumetric decrease of 35.5% (p = 0.109).Conclusions UAE for fibroids and adenomyosis can be performed with high technical success and low complication rates in a low-resource setting like Tanzania, resulting in significant symptom relief for patients. Building capacity for UAE has major public health implications not only for fibroids and adenomyosis, but can help address the region’s leading cause of maternal mortality, postpartum hemorrhage.
Identification of Predicting Diagnostic Gene Biomarkers through Machine Learning combined with Pan-cancer in Patients with Systemic Lupus Erythematosus
Authors: Zhan Jinfeng , Cheng Ruoying , Liu Qi , Zu Yuxin , Hu Kaibo , Xia Zhongbin 
Published: April 2023

Abstract
Background Early diagnosis of systemic lupus erythematosus (SLE) is crucial for treatment and reducing mortality. In this research, we set out to explore several important biomarkers for the diagnosis of SLE through machine learning combined with pan-cancer, and to investigate the significance of immune cell infiltration.Methods Gene expression profiles for three human SLE and control samples were downloaded from the GEO database. The LASSO regression model and support vector machine recursive feature elimination (SVM-RFE) analysiswere used to identify candidate biomarkers. The area under the receiver operating characteristic curve (AUC) value was obtained for the evaluation of the discriminations. The GSE20864 dataset was then further validated for the expression and diagnostic value of SLE biomarkers. The structure of 22 immune cell fractions in SLE was explored through the pooled cohort of CIBERSORT. The Cancer Genome Atlas (TCGA), Treatment-Applicable Research to Generate Effective Treatments (TARGET) and Genotype-Tissue Expression (GTEx) datasets are sources of pan-cancer and normal tissue data. And associated pan-cancer analysis was carried out.Results We identified a total of 52 differently expressed genes, of which 23 were significantly upregulated and 29 were significantly downregulated. After further screening, we found four genes as candidate biomarkers (FOS, IFI27, ANKH, and IGF2BP2). Three signature genes (ANKH, IFI27, IGF2BP2) are excellent diagnostic values in treat and control groups. In immune cell analysis, 11 immune cells showed significant differences between SLE patients and normal humans. We also analysed the effect of IGF2BP2 in the tumour process. The IGF2BP2 gene was low expressed in 9 tumors and high expressed in 22 tumors. In addition, we also found a strong correlation between IGF2BP2 expression in tumour cells and patient prognosis. Our study also indicates that IGF2BP2 may have the ability to predict the efficacy of ICI in the corresponding cancer.Conclusion We explored several important biomarkers for the diagnosis of SLE through machine learning and found that IGF2BP2 has a sufficient role in the immune microenvironment of most of the tumors.
A fluorescent probe based on aptamer gold nanoclusters for rapid detection of mercury ions
Authors: Wang Ying , Luo Shan , Jiang Ruina , Dong Quanyong , Sun Yingying , Qiao Xin , Li Wang , Xiong Ying , Chen Yanni , Yi Sili , Wen Qian 
Published: April 2023

Abstract
Mercuric ion (Hg2+) is a hazardous pollutant widely exist in living organisms, foods and environments with highly toxic and bio-accumulative properties. In this work, a fluorescent probe based on aptamer gold nanoclusters (apt-AuNCs) was prepared for ultrasensitive detection of Hg2+ in food. The fluorescence of apt-AuNCs was quenched in the presence of Hg2+ due to the strong metallophilic interactions between the 5d10 centers of Hg2+ and Au+. Under optimal conditions, the proposed fluorescent probe displayed a linear relationship with Hg2+ concentration in the range of 2~ 200 nM (R2 =0.9960), the limit of detection (LOD) at 0.0158 nM, which is below the national standard value of 25 nM for Hg2+ in food. Furthermore, the apt-AuNCs can be applied for the determination of Hg2+ in real food samples, with recoveries of 91.99% ~ 108.06%.
On regularity and asymptotic stability for semilinear nonlocal pseudo-parabolic equations
Authors: Trong Quyet Dao , Phuong Thanh Dang Thi 
Published: April 2023

Abstract
We deal with a class of nonlocal pseudo-parabolic equations involving strong nonlinearities. The questions on existence, regularity and stability of solutions are addressed by using local estimates, fixed point arguments, and the relation between the Hilbert scales and fractional Sobolev spaces.
Exploring the Relationship Between Servant Teacher Leadership Behaviour, Psychological Empowerment, and Cognitive Learning: Evidence from Universities of Pakistan
Authors: Yeying Yang , Quddoos Muhammad Umer , Latif Moazzam , Siddiqi Arslan Ahmad 
Published: April 2023

Abstract

The present study focuses on determining the suitability of servant-teacher leadership as a leadership approach for instructional purposes. The study specifically looks at how servant leadership affects followers' cognitive learning by considering the mediating role of follower psychological empowerment. Using a stratified random sampling technique, data was collected through a questionnaire from seven hundred graduates from various HEC-recognized universities. The results of the study show that servant teaching significantly improves students' cognitive learning and sense of empowerment. Additionally, the study found that psychological empowerment mediates the link between follower cognitive learning and servant leadership. The findings of this study provide new insight to instructors seeking to develop a learning environment that fosters students' learning outcomes. However, it is also important to consider how to ensure students' commitment and that they are being challenged. Future research may explore the impact of cultural values on students' behavior toward servant teachers.
Use of Adjuvant Rectal Diazepam with Oral Tadalafil for Treatment of Chronic Prostatitis/Chronic Pelvic Pain Syndrome
Authors: Herati Amin , Pil Ellen , Li Oscar , Engel Noah , Rabinowitz Matthew , Peña Vaness 
Published: April 2023

Abstract
Studies show oral Tadalafil and Diazepam suppositories each independently improve chronic prostatitis/chronic pelvic pain syndrome (CP/CPPS), leading providers to use medications simultaneously in the same patients. Our objective was to explore the efficacy of Tadalafil in CP/CPPS symptom management and whether co-administration of rectal Diazepam enhances potential benefit. A single-institution, retrospective chart review was performed on 63 adult males with CP/CPPS. NIH-Chronic Prostatitis Symptom Index (CPSI) questionnaires were collected before and after at least 45 days of treatment with either Tadalafil alone (n = 40) or Tadalafil with adjunctive Diazepam suppositories (n = 23). Both groups had similar baseline pain and urinary CPSI sub-scores, yet patients treated with Diazepam had significantly worse median quality of life sub-scores on both the initial (8.5 vs. 11, P = 0.01) and final (4 vs. 8.5 P = 0.02) surveys. For both groups, CPSI aggregated scores and pain, urinary, and quality of life sub-scores were significantly reduced compared to scores before treatment (P < 0.0001-0.02). However, the reduction in symptom scores was not significantly different with the addition of Diazepam suppositories (P = 0.47–0.94). Tadalafil, both with and without Diazepam, improved CP/CPPS symptom scores across all domains of the CPSI questionnaire. However, Diazepam suppositories do not confer additional benefit compared to Tadalafil therapy alone for CP/CPPS.
Global Trends and Hotspots in Narrative Medicine Studies: A Bibliometric Analysis
Authors: Li Rui , Wang Le 
Published: April 2023

Abstract
Background In recent decades, the overemphasis on technical aspects of precision medicine may neglect patients' personal feelings, leading to conflicts and tensions between doctors and patients. Narrative medicine is an interdisciplinary approach aimed at facilitating physician-patient communication, enhancing empathy, and improving the quality of medical care through storytelling. However, the global trends and hotspots in the field of narrative medicine remains unclear. To fill this gap, we conducted a bibliometric analysis of the global scientific publications in the field of narrative medicine, utilizing two visualization tools, CiteSpace and VOSviewer, on papers published in the Web of Science database between 2011–2021.Methods The study presented a bibliometric and visual analysis of the research status, global trends and hotspots in narrative medicine. Using the Web of Science Core Collection (WoSCC) as the data source, 736 articles published between 2011 and 2021 were retrieved and analyzed based on publications, authors, countries, institutions, journals, and keywords.Results Over the past decade, the number of publications in this field has steadily increased each year. The study found that American scholars contributed the most research papers in this field (369 papers) and that the United States is the key node in cooperation with UK, Canada, Italy and other countries. Furthermore, the most influential research team was found to be Columbia University (with 29 papers and 406 citations). The current research hotspots were classified into four clusters: narrative medicine education, consumer health information, health insurance and medical overuse. The function of education and the application of narrative medicine were identified as potential future research hotspots.Conclusion The current study suggested active cooperation among authors, institutions and countries. Among the four clusters, NM education are closely related with the other three clusters. Thus, more attention should be paid to the function of NM education.
Biomechanical Properties of Articular Cartilage in Different Regions and Sites of the Knee Joint: Acquisition of Osteochondral Allografts
Authors: Ma Yongsheng , Lin Qitai , Wang Xueding , Liu Yang , Yu Xiangyang , Ren Zhiyuan , Zhang Yuanyu , Guo Li , Wu Xiaogang , Zhang Xiangyu , Li Pengcui , Wei Xiaochun , Duan Wangping 
Published: April 2023

Abstract

Background: Osteochondral allograft (OCA) transplantation involves grafting of natural hyaline cartilage and supporting subchondral bone into the cartilage defect area of a joint to recover its biomechanical and tissue structure. However, differing biomechanical properties and donor-host matching may impair the integration of articular cartilage (AC). This study analyzed the biomechanical properties of the AC in different regions of different sites of the knee joint and provides a novel approach to OCA transplantation.
Methods： Intact stifle joints from skeletally mature pigs were collected from a local abattoir less than 8 h after slaughter. OCAs were collected from different regions of the joints. The patella and thetibial plateau were divided into medial and lateral regions, while the trochlea and femoral condyle were divided into six regions. The OCAs were analyzed and compared for Young’s modulus, the compressive modulus, and cartilage thickness.
Results:Young’s modulus, cartilage thickness and compressive modulus of OCA were significantly different in different regions of the joints. A negative correlation was observed between Young's modulus and the proportion of the subchondral bone (r=−0.4241, P<0.0001). Cartilage thickness was positively correlated with Young’s modulus (r=0.4473, P<0.0001) and the compressive modulus (r=0.3678, P<0.0001).
Conclusions: During OCA transplantation, OCAs may be xenotransplanted into similar regions, or at the closest possible regions to ensure consistency of the biomechanical properties and cartilage thickness of the donor and recipient and, thus, easy integration with the surrounding tissue. A 7-mm depth achieved a higher Young's modulus, and may represent the ideal length.
BAHD1 promotes osetosarcoma recurrence by enhancing tumor cell escape from chemotherapy-induced senescence
Authors: zhang liang , luo dan , zhang jian , zhu jun , gan yibo , Zhao Jianhua , Liu Mingyong 
Published: April 2023

Abstract
Objective
To investigate the role and mechanism of BAHD1 in the proliferation and migration of osteosarcoma cells escaping from senescence.
Methods
Osteosarcoma cell lines U2OS and MG63 were cultured in vitro. Doxorubicin (DOX) and cisplatin induced U2OS and MG63 into a senescent state. The medium without DOX and CIS was replaced for continuous culture. Some cells were observed to proliferate again, which were defined as senescence-escaped OS cells (SEOS). The DOX group and CIS group were divided respectively. The DOX and CIS groups are divided into: the parent U2OS group, the U2OS SEOS cells at the 9th passage group, the parent MG63 group, and the MG63 SEOS cells at the 9th passage group. The expression of BAHD1 was detected by qRT PCR, Western blot and immunofluorescence. Cell count, CCK-8 test, agarose clone formation test, flow cytometry, etc. were used to detect cell proliferation. Cell Migration Assay was used to detect cell migration. Tumor Xenografts was used to detect the ability of cells to form tumors. The effect of BAHD1 was detected by RNA interference assay.
Results
The expression of BAHD1, cell cycle related proteins YWHAZ, CCND1 and stem cell factor KLF4 were significantly increased in SEOS cells at the 9th passage group compared with the parent group. Cell cycle enters S phase more quickly. After knockdown of BAHD1 expression, the above results were significantly recalled, and the ability of migration, cloning and tumor formation was significantly reduced.
Conclusion
The increased expression of BAHD1 is crucial in the progression of osteosarcoma, which may be achieved by up regulating the cell cycle related proteins YWHAZ, CCND1 and stem cell factor KLF4.
The impact of non-drug treatments on the quality of life of patients with non-dementia vascular cognitive impairment
Authors: Novikova M. S. , Zakharov V. V. 
Published: April 2023

Introduction. Cognitive impairment significantly reduces the quality of life. This occurs already in the early stages of the disease. At present, the effect of non-drug treatment on mild cognitive impairment and the quality of life of patients has not been sufficiently studied.Aim. To evaluate the effect of a combination of non-drug treatments on the quality of life of patients with non-demented vascular cognitive impairment.Materials and methods. 60 patients with moderate cognitive impairment of vascular etiology were included in an open randomized trial. Patients of the first group (n = 30, mean age 63.7 ± 8.8) received a combination of non-drug methods (cognitive training, physical activity, meal planning) for one month. Patients of the second group (n = 30, mean age 64.2 ± 10.7) were informed about the advisability of a healthy diet and maintenance of mental and physical activity. Neuropsychological testing was carried out at the stage of inclusion, immediately after the course of treatment, after 6 months and one year after the start of the study.Results. Patients of the first group showed a statistically significant improvement in the quality of life (p < 0.05) one month after the start of the study. After 6 months and after a year, there were no significant differences with the first visit. When comparing the indicators of the second group with the initial visit, a decrease in the quality of life was noted throughout the follow- up (p < 0.05). The differences were statistically significant when comparing the two groups with each other at the time of the second visit, but at the third and fourth visits they lost statistical significance.Conclusion. The results obtained indicate that the use of a complex of non-drug methods of treatment for moderate vascular cognitive impairment improves the quality of life of patients.
Glycosaminoglycan-peptide complex: data on the mechanism of action and efficacy in osteoarthritis
Authors: Chichasova N. V. , Lila A. M. 
Published: April 2023

The article presents the modern definition of osteoarthritis (OA) not as a degenerative cartilage injury, but as a disease in which abnormal adaptive regenerative processes are activated, including pro-inflammatory pathways of the immune system, based on the clarification of the pathogenesis of the disease. An approach to the separation of various OA phenotypes is described. Various approaches to the tactics of pharmacological treatment of the disease are presented. The possibilities of the glycosaminoglycan-peptide complex to influence the state of chondrocytes and cartilage tissue in various experimental models of induced OA are described. And in the last experimental study, a positive effect of the drug on the clinical manifestations of 2 models of induced OA was demonstrated, a decrease in the concentration of CRP, interleukin 1β was recorded with an increase in the concentration of anti-inflammatory cytokines (interleukins 4 and 10), a significant decrease in the number of leukocytes in the synovial fluid, as well as a decrease in pathological changes in cartilage during histological examination, which it indicates that the drug exerts its effect directly in the tissues of the joint. Clinical studies have confirmed the analgesic and anti-inflammatory activity of the glycosaminoglycan-peptide complex in OA, although not all conducted in the twentieth century, the positive effect on joint pain and joint function was significantly better than placebo. The absence in these years of regulated criteria for inclusion in studies of the effectiveness of pharmacological drugs in OA, the introduction of new methods for assessing pain, function served as a prerequisite for conducting studies of the effectiveness and tolerability of the glycosaminoglycan-peptide complex at the present stage. Multicenter observational studies, which included massive groups of patients with OA of various localization, confirmed the presence of analgesic and anti-inflammatory activity in the drug, manifested during the 1st course of injections, showed that repeated courses of drug administration are necessary to achieve a more pronounced and stable effect. Data on an increase in the effect of combination therapy with diacerein and on the possibility of achieving an effect in patients with previous insufficient efficacy of other slow-acting symptomatic drugs are presented.