bluuebunny/crossref_metadata_2025_split huggingface dataset:
Morphofunctional changes at the active zone during synaptic vesicle exocytosis
Authors: Radecke Julika , Seeger Raphaela , Kádková Anna , Laugks Ulrike , Khosrozadeh Amin , Goldie Kenneth N , Lučić Vladan , Sørensen Jakob B , Zuber Benoît 
Published: March 2023

AbstractSynaptic vesicle (SV) fusion with the plasma membrane (PM) proceeds through intermediate steps that remain poorly resolved. The effect of persistent high or low exocytosis activity on intermediate steps remains unknown. Using spray‐mixing plunge‐freezing cryo‐electron tomography we observe events following synaptic stimulation at nanometer resolution in near‐native samples. Our data suggest that during the stage that immediately follows stimulation, termed early fusion, PM and SV membrane curvature changes to establish a point contact. The next stage—late fusion—shows fusion pore opening and SV collapse. During early fusion, proximal tethered SVs form additional tethers with the PM and increase the inter‐SV connector number. In the late‐fusion stage, PM‐proximal SVs lose their interconnections, allowing them to move toward the PM. Two SNAP‐25 mutations, one arresting and one disinhibiting spontaneous release, cause connector loss. The disinhibiting mutation causes loss of membrane‐proximal multiple‐tethered SVs. Overall, tether formation and connector dissolution are triggered by stimulation and respond to spontaneous fusion rate manipulation. These morphological observations likely correspond to SV transition from one functional pool to another.
Vitamin C promotes ACE2 degradation and protects against SARS‐CoV‐2 infection
Authors: Zuo Yibo , Zheng Zhijin , Huang Yingkang , He Jiuyi , Zang Lichao , Ren Tengfei , Cao Xinhua , Miao Ying , Yuan Yukang , Liu Yanli , Ma Feng , Dai Jianfeng , Tian Sheng , Ding Qiang , Zheng Hui 
Published: March 2023

AbstractACE2 is a major receptor for cellular entry of SARS‐CoV‐2. Despite advances in targeting ACE2 to inhibit SARS‐CoV‐2 binding, strategies to flexibly and sufficiently reduce ACE2 levels for the prevention of SARS‐CoV‐2 infection have not been explored. Here, we reveal vitamin C (VitC) administration as a potent strategy to prevent SARS‐CoV‐2 infection. VitC reduces ACE2 protein levels in a dose‐dependent manner, while even a partial reduction in ACE2 levels can greatly inhibit SARS‐CoV‐2 infection. Further studies reveal that USP50 is a crucial regulator of ACE2 levels. VitC blocks the USP50‐ACE2 interaction, thus promoting K48‐linked polyubiquitination of ACE2 at Lys788 and subsequent degradation of ACE2 without affecting its transcriptional expression. Importantly, VitC administration reduces host ACE2 levels and greatly blocks SARS‐CoV‐2 infection in mice. This study reveals that ACE2 protein levels are down‐regulated by an essential nutrient, VitC, thereby enhancing protection against infection of SARS‐CoV‐2 and its variants.
An innovative hematopoietic stem cell gene therapy approach benefits CLN1 disease in the mouse model
Authors: Peviani Marco , Das Sabyasachi , Patel Janki , Jno‐Charles Odella , Kumar Rajesh , Zguro Ana , Mathews Tyler D , Cabras Paolo , Milazzo Rita , Cavalca Eleonora , Poletti Valentina , Biffi Alessandra 
Published: March 2023

AbstractHematopoietic stem and progenitor cells (HSPCs) can establish a long‐lasting microglia‐like progeny in the central nervous system of properly myeloablated hosts. We exploited this approach to treat the severe CLN1 neurodegenerative disorder, which is the most aggressive form of neuronal ceroid lipofuscinoses due to palmitoyl‐protein thioesterase‐1 (PPT1) deficiency. We here provide the first evidence that (i) transplantation of wild‐type HSPCs exerts partial but long‐lasting mitigation of CLN1 symptoms; (ii) transplantation of HSPCs over‐expressing hPPT1 by lentiviral gene transfer enhances the therapeutic benefit of HSPCs transplant, with first demonstration of such a dose–effect benefit for a purely neurodegenerative condition like CLN1 disease; (iii) transplantation of hPPT1 over‐expressing HSPCs by a novel intracerebroventricular (ICV) approach is sufficient to transiently ameliorate CLN1‐symptoms in the absence of hematopoietic tissue engraftment of the transduced cells; and (iv) combinatorial transplantation of transduced HSPCs intravenously and ICV results in a robust therapeutic benefit, particularly on symptomatic animals. Overall, these findings provide first evidence of efficacy and feasibility of this novel approach to treat CLN1 disease and possibly other neurodegenerative conditions, paving the way for its future clinical application.
Lukács: Philosoph eines Jahrhunderts
Authors: Müller-Funk Wolfgang 
Published: March 2023

AbstractGyörgy Lukács is an intellectual ‘heavyweight’ of that century which, since Eric Hobsbawm, we have called a short one, although the years from 1900 to 1914 and from 1989 to 2000 do not fit into the picture of a century that was defined by war and civil war, by ideological trench warfare, by the Shoah and the Gulag, by the Cold War and decolonisation, by new art forms and media. With his early books The Soul and the Forms and his Theory of the Novel, he made an enduring contribution to aesthetic modernity; with History and Class Consciousness, his first work in the footsteps of Hegel and Marx, he made a pioneering attempt to place Marxism on philosophical feet and, like Antonio Gramsci or Karl Korsch, to correct the theoretically non-ambitious, Darwinian-influenced Marxism of the last quarter of the 19th century. It is seen in this essay as a work of discontinuity in continuity.
Aplikasi Sport Human Connection (SHC) sebagai Marketplace untuk Publikasi dan Pemasaran Layanan Pelatih Olahraga
Authors: Supriyadi Supriyadi , Adi Sapto , Fathoni Abi Fajar 
Published: March 2023

Abstract: The financial achievements of outstanding athletes are not directly proportional to the finances of the coaches who have driven these athletes' careers. It is necessary to develop an application that can link the influence between athlete achievement and coach marketing. When athletes get achievements, coaches also get additional job opportunities to increase their financial income. The purpose of this research and development is to develop the Sport Human Connection (SHC) application as a marketplace for publication and marketing of sports coach services. Research and development was chosen for this research method by involving 40 samples of sports trainers spread across various provinces in Indonesia. Sports trainers state that the SHC application with all its features can help the publication and marketing of the sports coach profession. As well as significantly, the marketing of sports trainers is more effective by using the SHC application than using the conventional methods used by sports coaches before. It can be concluded that the SHC application can help the publication and marketing of sports trainers which can provide additional job opportunities and financial income. For this reason, it is necessary to hold an audience with the Indonesian government so that the use of the SHC application obtains a policy and can be disseminated to sports coaches throughout Indonesia.
Abstrak: Financial atlet berprestasi belum berbanding lurus dengan financial pelatih-pelatih yang mengantarkan karier atlet tersebut. Perlu dikembangkan sebuah aplikasi yang bisa menghubungkan pengaruh antara prestasi atlet dengan pemasaran pelatih. Ketika Atlet memperoleh prestasi, pelatih juga mendapat peluang pekerjaan tambahan untuk meningkatkan pendapatan finansialnya. Tujuan penelitian dan pengembangan ini adalah mengembangkan aplikasi Sport Human Connection (SHC) sebagai marketplace untuk publikasi dan pemasaran layanan pelatih olahraga. Research and development dipilih untuk metode penelitian ini dengan melibatkan 40 sampel pelatih olahraga yang tersebar di berbagai provinsi di Indonesia. Pelatih olahraga menyatakan bahwa aplikasi SHC dengan semua fiturnya mampu membantu publikasi dan pemasaran profesi pelatih olahraga. Serta secara signifikan, pemasaran pelatih olahraga lebih efektif dengan menggunakan aplikasi SHC daripada menggunakan cara konvensional yang digunakan para pelatih olahraga sebelumnya. Dapat disimpulkan bahwa aplikasi SHC bisa membantu publikasi dan pemasaran pelatih olahraga yang mampu memberikan peluang pekerjaan dan pendapatan finansial tambahan. Untuk itu perlu dilakukan audiensi dengan pemerintah Indonesia agar penggunaan aplikasi SHC memperoleh kebijakan dan dapat disosialisasikan ke pelatih olahraga di seluruh penjuru Indonesia.
Hubungan Antara Kecanduan E-Sport Dengan Stres Pada SMP Negeri 22 Malang
Authors: Darmawan Ahmad Irfan , Adi Sapto , Supriyadi Supriyadi , Abdullah Ahmad 
Published: March 2023

Abstract: E-Sport addiction, lack of control over the excessive use of E-Sport games causes anxious people to become addicted to E-Sports psychologically and emotionally. Adolescence is a period of transition, adolescents are prone to stress, and excessive online games are an indicator of stress. The purpose of this study was to determine the relationship between E-Sport addiction and stress in students of SMP Negeri 22 Malang. This research is an analytic survey with a sample taken using a probability sampling technique using the Slovin formula as many as 82 people. The instrument used in this study was a questionnaire. The results of the study used the Pearson Correlation Product Moment statistical test analysis with p equal to 0.00. Conclusion This study shows that there is a relationship between online game addiction (E-Sport) and stress at SMP Negeri 22 Malang.
Abstrak: Kecanduan E-Sport, kurangnya kontrol atas penggunaan game E-Sport yang berlebihan menyebabkan orang yang cemas menjadi kecanduan E-Sport secara psikologis dan emosional. Masa remaja merupakan masa transisi, remaja rentan mengalami stres, dan game online yang berlebihan merupakan salah satu indikator stres. Tujuan penelitian ini adalah untuk mengetahui hubungan kecanduan E-Sport dengan stres pada siswa siswi SMP Negeri 22 Malang. Penelitian ini bersifat survei analitik dengan sampel diambil dengan teknik pengambilan probability sampling menggunakan rumus slovin yaitu sebanyak 82 orang. Instrumen yang digunakan dalam penelitian ini adalah kuesioner. Hasil penelitian menggunakan analisis uji statistik Pearson Correlation Product Moment dengan nilai p sama dengan 0,00. Kesimpulan Studi ini menunjukkan bahwa ada hubungan antara kecanduan game online (E-Sport) dengan stres di SMP Negeri 22 Malang.
Evaluación de la fertilidad de los suelos en sistemas agroforestal con Palma Africana (Elaeis guineensis Jacq.)
Authors: Furcal-Beriguete Parménides , Robles-Argüello Zeydi , Salazar-Díaz Ricardo 
Published: March 2023

Se muestrearon cinco parcelas de tres años, establecidas por la Iniciativa Osa & Golfito (INOGO), cada parcela constaba con dos tratamientos los cuales fueron sistema agroforestal (SAF) integrado por E. guineensis, C. megalantha, T. cacao y Musa sp, y el monocultivo de E. guineensis; cuyo objetivo fue evaluar el efecto de un sistema productivo agroforestal con Elaeis guineensis sobre la fertilidad fisicoquímica del suelo. Se determinó la densidad aparente del suelo y se extrajeron muestras compuestas en dos profundidades, obteniéndose el contenido de nutrientes, la materia orgánica, la densidad de partículas y la porosidad. Los datos fueron analizados estadísticamente en donde las parcelas fungieron como bloques, sin mostrar diferencias significativas para algunas variables edáficas y elementos evaluados; sin embargo, el contenido de fósforo fue significativamente más alto en el SAF y disminuyó con la profundidad del suelo 0 – 20 cm a 20 – 40 cm, el magnesio aumentó estadísticamente del año 2019 al 2020, igualmente que la materia orgánica, el carbono y el nitrógeno total y fueron superiores en la superficie (0 -20 cm) que a la profundidad 20 - 40 cm.
A hybrid system bases on silica-alumina and Keggin heteropolyacids as catalyst in the suitable 2-(2-furyl)-chromones and chromanones synthesis
Authors: V. Palermo  , D. Ruiz  , A. Sathicq  , P. Vázquez  , G. Romanelli  
Published: March 2023

Molybdophosphoric acid/silica-alumina composites are synthesized a through a process described, in which the heteropolyacid was impregnated on different silica-aluminas, obtained by sol-gel. Three different techniques were used to prepare the samples. The catalysts were prepared by incipient wetness impregnation and different thermal treatments were applied. The hybrid systems were characterized by using SBET, DRX, XRD and acidity measurements.
The catalytic activity of these materials was tested in the solvent-free cyclization of 1-(2-hydroxyphenyl)-3-(2-furyl)-1,3-propanedione to 2-(2-furyl)-chromones. The transformation gives very good yields of product, free of secondary products. Environmental benign procedure, and easy catalyst separation, is relevant features of this methodology. In this way the catalyst can be used and reused six cycles without loss of catalytic activity. The most active catalyst was also used in the solvent-free cyclization of 1-(2-hydroxyphenyl)-3-(2-furyl)-2-propen-1-one and the methodology can be extended to the synthesis of other 2-(2-(furyl)-chromones and chromanones. The green context for this new procedure was confirmed by greenmetrics parameters.
KEBERLANGSUNGAN USAHA UMKM BERDASARKAN MANAJEMEN RISIKO KEUANGAN DI DAERAH LAHAN BASAH KOTA BANJARMASIN
Authors: Jikrillah Sufi , Ziyad Muhammad , Stiadi Doni 
Published: March 2023

Abstract
The purpose of this research is to identify the process of financial risk management, to assess the financial risks that may arise and to analyze the strategies that will be carried out by MSMEs in dealing with or managing these financial risks. This research was conducted in accordance with the risk management process (ISO 31000:2018), namely financial risk identification, financial risk analysis, financial risk evaluation and financial risk treatment.
In this study, using Census Sampling, which is a sampling method when all members of the population are used as samples. The number of population and sample is the same (saturated sample) from this study are MSME business actors around the coast of the Martapura river in Banjarmasin City. The number of samples used in this study were 50 MSMEs located on the coast of the Martapura river in Banjarmasin City.
The results of the risk analysis show that there is capital risk, and liquidity risk is included in the categoryUnacceptable (red)that is Financial risk treatment must be carried out immediately even though the costs are high because it involves the continuity of the company so that capital risk and liquidity risk become the main focus and also because these financial risks have a major impact on MSMEs. Market risk and credit risk fall into the categoryUnacceptable (red) then risk treatment needs to be done so that the financial risk is minimized.
Keywords: Finance Risk Management, SWOT Analysis, SMEs.
ABSTRAK
Tujuan dari penelitian ini adalah mengidentifikasi proses manajemen risiko keuangan, melakukan penilaian terhadap risiko keuangan yang kemungkinan muncul dan melakukan analisa terhadap strategi yang akan dilakukan oleh UMKM dalam menangani ataupun mengelola risiko keuangan tersebut. Penelitian ini dilakukan sesuai dengan proses manajemen risiko (ISO 31000:2018) yaitu identifikasi risiko keuangan, analisis risiko keuangan, evaluasi risiko keuangan dan perlakuan risiko keuangan.
Pada penelitian ini menggunakan Sampling Sensus yaitu metode penarikan sampel bila semua anggota populasi dijadikan sebagai sampel. Jumlah populasi dan sampel adalah sama (sampel jenuh) dari penelitian ini adalah pelaku usaha UMKM sekitar pesisir sungai Martapura di Kota Banjarmasin. Jumlah sampel yang digunakan dalam penelitian ini adalah sebanyak 50 UMKM yang berada di pesisir sungai Martapura di Kota Banjarmasin.
Hasil analisis risiko terdapat risiko permodalan, dan risiko likuiditas termasuk dalam kategori Unacceptable (merah) yaitu perlakuan risiko keuangan harus segera dilakukan walaupun biaya mahal karena menyangkut kelangsungan perusahaan sehinga risiko permodalan dan risiko likuiditas menjadi fokus utama dan juga dikarenakan risiko keuangan tersebut berdampak besar pada UMKM. Risiko pasar dan risiko kredit masuk dalam kategori Unacceptable (merah) maka perlakuan risiko perlu dilakukan agar risiko keuangan tersebut menjadi minimal.
Kata Kunci : Manajemen Risiko Keuangan, Analisis SWOT, UMKM
The Anti-Senescence Effect and Mechanism of 17β-estradiol on Pelvic Organ Prolapse Derived Fibroblasts
Authors: Cheng Juan , Zhao Zhiwei , Wang Ling , Wen Jirui , Miao Yali , Wu Jiang 
Published: March 2023

Abstract
Background:
Recently, low estrogen and the age at menopause as independent risk factors for Pelvic Organ Prolapse (POP) were attracting high attention. In clinical practice, pre-/post-operative Local Estrogen Therapy (LET) shown effectiveness in alleviating POP symptoms. However, there is lack of scientific evidence to support the validity of these claims. Therefore, this study aimed to investigate the anti-senescence effect and mechanism of 17β-estradiol on POP derived fibroblasts.
Methods:
The primary fibroblasts cells were isolated and cultured form surgical POP samples (n = 8, age from 50–75), the passage-0 cells confluence at 80% takes about 15 days and the passage 3–5 cells were used for further test. Immunocytochemistry was used to characterize the primary fibroblasts, CCK8 assay was used to test the cell proliferative capacity and the Senescence-Associated β-Galactosidase (SA-β Gal) Staining was tested to calculate the senescence rate of fibroblasts. Moreover, western blotting was used to detect the expression of COL-I, COL-III, p16INK4A, p21, p-53, SIRT-1 and LC3-I/II protein. In addition, Transmission Electron Microscope (TEM) was used to observe the ultrastructure of fibroblasts.
Results:
The results showed that 17β-estradiol (E2) significantly promoted the POP derived-fibroblasts proliferation and reduced the staining rate of senescence-associated-β-galactosidase (SA-β-Gal), markedly enhanced the extracellular matrix protein COL-I and COL-III accompanied by the inhibition of senescent protein P16INK4a, as well as improved the cells autophagy and metabolic activity. In addition, E2 significantly up-regulated the anti-aging protein SIRT1 and markedly down-regulated p53 and p21, indicating the anti-senescence mechanism of E2 through mediated the Sirt1/p53/p21 axis pathway.
Conclusion:
We provide preliminary evidence that anti-aging effect and mechanism of estrogen on POP fibroblasts, hoping to provide a theoretical basis for estrogen against POP senescence, guide the clinical application and local administration of estrogen on POP treatment, thereby improve long-term maintenance and rejuvenation of the pelvic floor connective tissue.
Validation of an instrument to measure community capacity building for prevention
Authors: Röding Dominik , Birgel Vera , Walter Ulla 
Published: March 2023

Abstract
Aim: In Germany, the CTC-EFF study, a replication study of the U.S. Community Youth Development Study (CYDS), was rolled out in 2021. This article aims to examine the validity of a translated and adapted version of an instrument that measures five constructs of community capacity for prevention.
Subject and Methods: Twenty-two a-priori matched intervention and comparison communities participate in the study. Two to ten community key informants (n = 182) were interviewed for each community. Exploratory (EFA) and confirmatory factor analyses (CFA) were conducted to assess structural validity. Reliability was assessed at the individual level using McDonald’s omega and at the community level using a generalizability coefficient (GC). Inter-rater agreement was measured using an intraclass correlation coefficient (ICC). Concurrent construct validity was assessed using bivariate correlations and mean comparisons.
Results: CFA showed good model fit (CFI = 0.964) for the hypothesized five constructs model. Reliability analyses showed good internal consistency at the individual level (omega = 0.86 – 0.87) and insufficient to moderate reliability at the community level (CG = 0.00 – 0.62). Inter-rater agreement ranged from insufficient to substantial (ICC = 0.02 - 0.32). Consistent with theory, three constructs correlated positively at the individual and community level (r = 0.24 - 0.42). There are no associations between these constructs and interviewer ratings about the respondents’ cooperativeness, trustworthiness, and understanding.
Conclusion: Three of the five constructs were measured with good validity. Two constructs show insufficient reliability. These will be revised until the next CTC-EFF data collection wave. Further validation is indicated.
Trial registration: This study was registered with the German Clinical Trial Register: DRKS00022819 on Aug 18, 2021.
A Note on the Global Asymptotic Stability
Authors: Hejri Mohammad 
Published: March 2023

Abstract
This paper addresses the classical problem of global asymptotic
stability analysis of nonlinear systems. The note followed in this
paper looks at the necessity of an additional condition on the
derivative of the Lyapunov function to achieve the global asymptotic
stability missed in the original statement. The classical theorem
and its proof, selected from some seminal references, are discussed.
It is shown that the available proofs have been developed for a
weaker version of the global asymptotic stability called as
semi-global asymptotic stability by which state convergence is
guaranteed within each compact set of entire space. The new
additional condition extends the existing results to meet the main
definition of global asymptotic stability in which the state
convergence is guaranteed when the initial condition approaches
infinity in the entire space. Numerical results illustrate the
effectiveness of the proposed additional condition.
Direct Gas-Phase Formation of HCOOH through Reaction of Criegee Intermediates with Formaldehyde
Authors: Luo Pei-Ling , Chen I-Yun 
Published: March 2023

Abstract
Ozonolysis of isoprene is considered to be an important source of formic acid (HCOOH), but its underlying reaction mechanisms related to formic acid formation are poorly understood. Here, we report the kinetic and product studies of the reaction between the simplest Criegee intermediate (CH2OO) and formaldehyde (HCHO), both of which are the primary products formed in ozonolysis of isoprene. In addition to evaluation of the temperature and pressure dependencies of the rate coefficients, we determine the branching yields of the products HCOOH + HCHO and CO + H2O + HCHO by simultaneous determination of HCOOH and CO with synchronized two-color time resolved dual-comb spectroscopy. A high yield of HCOOH is obtained up to 40–50% under varied conditions. This work indicates that CH2OO + HCHO would play a critical role to explain high levels of atmospheric HCOOH, particularly in the upper troposphere and lower stratosphere.
Simulation of the heat transfer of a bi-tubular heat exchanger with a hemispherical end
Authors: Imen Dhahri 
Published: March 2023

Abstract
In this study, different heat transfer mechanisms and flow conditions were investigated to determine the temperature distribution. For this purpose, we proposed a coaxial tubular heat exchanger with a hemispherical end for different thermal parameters. Our attention was focused on the effect of geometry on the thermal and dynamic characteristics of the flow and on the performance of the studied heat exchanger. The results obtained show a qualitative agreement between those obtained and those found in the literature with a difference mainly due to the difference in geometry (the hemispherical end of the heat exchanger) and the code used.