bluuebunny/crossref_metadata_2025_split huggingface dataset:
Three-dimensional reconstruction of lymphatics using a crown-rump length 30 mm human fetus
Authors: Huang Shu , Zhang Hechen , Zhao Peng , Chen Xinlin , Yan Gen , Jin Zhe-Wu 
Published: June 2023

Abstract
Few lymphatic morphological studies have been conducted to describe human development compared to the number of vascular studies. In this study, serial sections from a 30-mm human fetus were used to observe the differentiation of the lymphatics and blood vessels through D2-40 immunohistochemical staining and three-dimensional reconstruction to reflect the morphological characteristics. We found a connection between the lymphatics of the left jugular lymph sac flowing into the left brachiocephalic vein but not between the thoracic duct (TD) and left venous angle. We found a lymphatic plexus in the sacral region, which was located between L4 and S1. Moreover, a pair of paravertebral longitudinal lymphatics originated near the CC and collected lymphatics that accompanied the posterior intercostal blood vessels and finally flowed into the TD. The left lymphatics had a slightly greater advantage than that of the right lymphatics. In contrast, the venous system on the right had a greater advantage than that on the left. In addition, a pair of paravertebral longitudinal lymphatics may have been described as the thoracic duct.
Effect of Cr content on hot corrosion behavior of Inconel alloys in molten LiCl-Li2O
Authors: Lim Kyu-Seok , Choi Woo-Seok , Kim Wan-Bae , Cho Soo-Haeng , Lee Jong-Hyeon 
Published: June 2023

Abstract
The hot corrosion behaviors of Inconel alloys with different Cr contents (Inconel 600, 601, and 690), which are used widely in nuclear plants, were investigated in molten LiCl-Li2O salts. The hot corrosion behaviors were studied by measuring the mass and attack depth changes, surface and cross-sectional morphologies and elemental distributions, and compositional changes at the subscale and substrate scale as well as the spalled oxide scale. At 288 h, the weight losses of Inconel 601 and Inconel 690 were approximately four and twelve times higher, respectively, than that of Inconel 600. The corrosion products of all tested alloys were Cr2O3, NiO, and FeCr2O4. Inconel 600, which exhibited a dense and continuous external corrosion layer and an internal corrosion layer with localized corrosion behavior, exhibited superior corrosion resistance compared with those of Inconel 601 and 690, which showed a spalled external corrosion layer and an internal corrosion layer with uniform corrosion behavior. Thus, the corrosion resistance of the Inconel alloys tested in the hot lithium molten salts in an oxidizing atmosphere is closely related to the contents of the primary alloying elements in the alloys. Of the various alloys analyzed in this study, Inconel 600 exhibited the highest corrosion resistance. Thus, a Cr content of 16.30 wt% or less, Ni content of at least 73.66 wt%, and Fe content considerably lower than 8.15 wt% can result in excellent corrosion resistance.
primerJinn – a tool for rationally designing multiplex PCR primer sets and in silico PCR
Authors: Limberis Jason D , Metcalfe John Z 
Published: June 2023

Abstract
Background
Multiplex PCR amplifies numerous targets in a single tube reaction and is essential in molecular biology and clinical diagnostics. One of its most important applications is in the targeted sequencing of pathogens. Despite this importance, few tools are available for designing multiplex primers.
Results
We developed primerJinn, a tool that designs a set of multiplex primers and allows for the in silico PCR evaluation of primer sets against numerous input genomes. We used primerJinn to create a multiplex PCR for the sequencing of drug resistance-conferring gene regions from Mycobacterium tuberculosis, which were then successfully sequenced.
Conclusions
primerJinn provides a user-friendly, efficient, and accurate method for designing multiplex PCR primers and performing in silico PCR. It can be used for various applications in molecular biology and bioinformatics research, including the design of assays for amplifying and sequencing drug-resistance-conferring regions in important pathogens.
CTGF regulates mineralization in human mature chondrocyte by controlling Pit-1 and modulating ANK via the BMP/Smad signaling
Authors: Xiao Peng , Zhu Yunong , Xu Hongrui , Li Junlei , Tao Angui , Wang Hongji , Cheng Dong , Dou Xiaojie , Rao Xiangang , Guo Lin 
Published: June 2023

Abstract
Objective: Connective tissue growth factor (CTGF) has a potent proliferative, differentiated, and mineral effect, and is thought to be involved in cartilage mineralization in Osteoarthritis (OA). However, little is known about the mechanism of chondrocytic mineralization that is induced by Connective tissue growth factor (CTGF). As a crucial mediator in regulating mineral responses, the type III phosphate transporter 1 (Pit-1) has been implicated in the pathogenesis of articular mineralization. The aim of this study was, therefore, to determine whether Connective tissue growth factor regulates the formation of mature chondrocyte mineralization and the mechanisms underlying such mineralization.
Methods: The effect of Connective tissue growth factor (CTGF) was analyzed with human C-28/I2 chondrocyte. The chondrocytes were treated with Connective tissue growth factor (CTGF) or related inhibitor, and transfected with Overexpression and siRNA transfection of the type III phosphate transporter 1(Pit-1). The chondrocytes were subjected to Alizarin red S staining, PiPer phosphate assay kit, Alkaline Phosphatase Diethanolamine Activity Kit, ELISA, RT-PCR or Western blot analysis.
Results: Stimulation with Connective tissue growth factor (CTGF) markedly upregulated expression of the type III phosphate transporter 1(Pit-1) and levels of mineralization in the chondrocyte through activation of α5β1 integrin and BMP/Samd1/5/8 signaling pathways. Moreover, treatment of overexpressed Pit-1 could significantly upregulate expression of multipass transmembrane ankylosis (ANK) transporter in the cells. Blockade of CTGF receptor using α5β1 Integrin blocking antibody significantly suppressed the expression of Pit-1 and ANK transporter, and the events of chondrocyte mineralization.
Conclusions: Our data indicate that Connective tissue growth factor (CTGF) playing a critical role in the chondrocyte mineralization is dependent on the expression of the type III phosphate transporter 1(Pit-1) and multipass transmembrane ankylosis (ANK) transporter, while inhibition of CTGF activity might serve as a novel therapeutic approach in the management of OA.
Bioengineering novel AAV9-mGULO-GT for multi-disease gene therapy: Targeting mutated GULO expression to cure scurvy and brain diseases.
Authors: Liu Jing , Kumar Manish , Guo Jing , Huang Luyuan , Zhang Zhen , WANG Yaofeng , Luo Rongping , chu shilong , Chen Shuyan , Liu Rongrong , Yu Jinjin , Lin Runxia , yu shengyong , zhou chunhua , Liu Jinpeng , Li Yusha , Das Pratteek , Zhang Jitian , Li Yi , Zhu Ping , Pei Duanqing 
Published: June 2023

Abstract
Current clinical breakthroughs in gene therapy have brought adeno-associated virus (AAV) vectors to the forefront of gene delivery systems. Vitamin C deficiency due to GULO mutations is a genetic disorder affecting guinea pigs and humans. In our study, we used AAV9-mGULO-GT to deliver the mouse GULO gene to guinea pigs and restore Vc synthesis in affected tissues, including the liver and brain. AAV9-mGULO-GT treatment significantly improved survival rates and bone health compared to non-treated and Vc-treated groups. Dot blot analysis confirmed restored Vc content in various parts of the brain. Additionally, micro-CT imaging showcased significant enhancements in bone mineral density, content, width, and cortical thickness. Further, RNA sequencing and immunological studies of organs validated the successful restoration of Vc synthesis. These findings highlight the potential of AAV9-mGULO-GT as a therapeutic option for GULO-related scurvy and other genetic disorders. The success of our study underscores the importance of advanced targeting and gene rescue systems in developing effective therapies for genetic disorders in clinical applications.
Fire behavior and mechanical properties of phosphorus-containing silica gel coated ammonium polyphosphate as fire retardants in thermoplastic polyurethane (TPU)
Authors: Zheng Ran , Li Mengqi , Chen Yajun , Hao Fenghao , Xu Bo 
Published: June 2023

Abstract
To enhance its properties, ammonium polyphosphate (APP) was subjected to modification using a synthesized phosphorus-containing silica gel, resulting in MAPP. Ammonium polyphosphate (APP) was modified by synthesized phosphorus-containing silica gel, resulting in MAPP, which was then used to form thermoplastic polyurethane composites. MAPP exhibited better thermal stability with a smaller dispersion size, lower initial decomposition temperature and better resistance to heat at high temperatures than APP. At the same time, MAPP showed more obvious advantages in improving the char yield, flame retardant performance and mechanical properties of TPU. TPU/5%MAPP showed a limiting oxygen index of 29.1% during burning tests, significantly better than TPU/5%APP (27.3%). The cone calorimeter test results also indicated that the incorporation of MAPP resulted in a significant reduction in key parameters such as peak heat release rate, total heat release, and total smoke release 爱and an increase of the char yield of TPU composite. The TG result showed that the Tmax of the TPU/5%MAPP composite increased and the mass loss rate decreased compared with that of TPU/5%APP. Moreover, the tensile strength of the TPU/5%MAPP composite increased by 18.5% compared with the TPU/5%APP composite due to the improved dispersibility of MAPP than APP. An analysis of the fire resistance mechanism showed that the improved flame retardance in the TPU/5%MAPP composite resulted from the structural changes in the char residue. This study provides an efficient and extensible approach for the synthesis of high-performance flame retardant TPU materials.
Flexible CB Co-Planar Wave Based Hinge Type Antenna for Breast Cancer Detection
Authors: Kulandhaisamy Indhumathi , Peng Sheng , Chakrabarti Tulika , Kshirsagar Pravin Ramdas , Chakrabarti Prasun , Margala Martin , Unhelkar Bhuvan 
Published: June 2023

Abstract
This paper presents hinge-shaped conductor backed (CB) monopole antenna designed to operate at 5.8 GHz frequency in the frequency range of 4 to 8 GHz with reflection co-efficient below − 29 dB with impedance as 50Ω. The wearable flexible antenna is of the dimensions 10mm X 10 mm. An array of 4 antenna model has also been tested for analysis. Kapton polyimide (substrate) and copper materials were used for the fabrication process. Flexible monopole antennas on a Kapton polyimide are designed to be in contact with the breast tissues with respective dielectric models. The flexible Kapton material with antennas is to be placed on the breast phantom which makes the detection easier. The proposed model is eco-friendly, cost effective and reliable product to be readily launched.
Pulmonary Mucormycosis in An elderly Acute Myeloid Leukemia Patient: a Case Report
Authors: Luo Bingqing , Chen Ying , Tan Xiaoyan , Chen Lin 
Published: June 2023

Abstract
Mucormycosis is a rare but fatal opportunistic fungal infection. Patients with hematologic malignancies who use immunosuppressant and glucocorticoid extensively are susceptible to mucormycosis. We report a case of an elderly patient with acute myeloid leukemia (AML) who infected with pulmonary mucormycosis during chemotherapy. With a good balance of chemotherapy-induced myelosuppression and control of mucorales infection, azactidine combined with venetoclax gave the patient a complete remission of leukemia and pulmonary mucormycosis was well controlled. But due to poor compliance, the patient died of asphyxiation as a result of aspiration of pulmonary necrotic substances after discharge at home. To our knowledge, this is the first case report of an elderly patient with AML complicated with pulmonary mucormycosis dying of asphyxia from necrotic pulmonary substances. Proper precautions to should be taken in the management mucormycosis.
Risk Evaluation on the Pcbs and Ocps Residues in the Water of Irtysh River
Authors: Tang Shi-Zhan , Wang Hai-Tao , Chen Zhong-Xiang , Hu Yao-Peng , Hao Qi-Rui , Wang Ji-Long , Qin Dong-Li , Wang Peng 
Published: June 2023

Abstract

To enhance the prevention and control of OCPs and PCBs pollution, the status of pollution by both in the Irtysh River was analyzed, and a risk evaluation was made on it. The pollutant residue amounts at the 18 sampling sites were shown as follows, including α-HCH: ND − 8.2269 ng/L, δ-HCH: ND − 6.7385 ng/L, β-HCH: ND − 5.2714 ng/L, γ-HCH: ND − 0.1320 ng/L, PCB 28: ND − 0.4111 ng/L, Aldrin: ND − 3.7320 ng/L, Dieldrin: ND − 4.8551 ng/L and Endrin: ND − 6.1473 ng/L. It was found from the source of OCPs that HCHs came from the historical use of industrial HCHs, while Aldrin, Dieldrin and Endrin came from the use of pesticides. By PCA (principal component analysis), three principal components were extracted. The total variance explained came to 95.98%, a result of the use of HCHs, the variation in OCPs materials and the research about pest attack features in the region. Further ecological risk evaluations indicated that affected by the use of pesticides, the ecological risk of OCPs at the upper reaches of Irtysh River was higher than that at other sites. It was necessary to extend the test objects, test types and test cycles to safeguard the ecological security and human health.
Active Control of Shaft Torsional Vibration with a Dynamic Torque Actuator
Authors: Luo Qing , Fan Wen-Kun , Hu Jie , Dong Jia-Xin , Wang Jiao 
Published: June 2023

Abstract
Multi-harmonic fluctuations from the output torque of an engine, propeller, or other power source constitute the main source of torsional vibration of a shafting system, and stimulate other coupled vibrations. This paper introduces a new method to address torsional vibrations of shafting systems based on line spectrum active control. A multi-body dynamic analysis model is used to reveal the coupling characteristics of torsional vibration and vibration in other directions. In addition, a new cross-coupled multi-channel line spectrum active control algorithm is proposed alongside a new type of torque actuator where the internal electromagnetic structure contains a rotating stator and rotor. These components are connected with springs, and the whole assembly can rotate with the shafting. The dynamic torque is issued according to the command of the active control system in order to suppress the torque fluctuation due to the power sources, which in turn greatly reduces the torsional vibration of the shafting.
Use of electronic devices in leisure time modifies the prevalence and factors associated with sedentary behavior derived exclusively from excessive television viewing among Brazilian adults
Authors: Bertuol Cecília , Silveira Murilo Henrique Corrêa , Krug Rodrigo de Rosso , Kupske Juliedy Waldow , Mielke Grégore Iven , Duca Giovani Firpo Del 
Published: June 2023

Abstract
The growing interest in the study of sedentary behavior is justified by its increasing presence in people's daily lives, particularly in leisure time. The aim of this study was to compare the prevalence and factors associated with sedentary behavior derived exclusively from TV time and from its combination with the time spent using other electronic devices among Brazilian adults (n = 52,443). This cross-sectional study used data from the Vigitel survey (2019), which included subjects ≥ 18 years old who resided in the capitals of the 26 Brazilian states and Federal District. High TV time (≥ 4 h/day), and its combination with computer, tablet, or cell phone use (≥ 4 h/day), as well as sociodemographic, behavioral, and health characteristics were self-reported. Adjusted logistic regression was used to estimate odds ratios and 95% confidence intervals (95%CI). The prevalence of high sedentary behavior almost tripled when TV viewing was added to the time spent using other electronic devices (from 12.2%; 95%CI: 11.6; 12.8, to 34.7%; 95%CI: 33.8; 35.6), notably among the youngest (32.0 percentage points). Individuals living without a partner, who smoked, consumed alcohol and processed foods excessively, were physically inactive, and had hypertension were more likely to have both outcomes than their counterparts. Older and less educated individuals were more likely to spend excessive time watching TV and less likely to have high use of other electronic devices in addition to TV viewing than their peers. Including computer, tablet, or cell phone led to an increase in the prevalence of high sedentary behavior. The magnitude and direction of the associations of age and education with high sedentary behavior varied according to the method how high sedentary behavior was defined. Projects, programs, and policies must consider the different indicators of sedentary behavior in monitoring and promoting a healthier lifestyle.
Exploration of antibody-mediated immunity in chronic histiocytic intervillositis reveals no evidence of maternal anti-fetal rejection
Authors: Brady Chloe A , Ford Laura B , Zou Zhiyong , Crocker Ian P , Heazell Alexander E P 
Published: June 2023

Abstract

Chronic histiocytic intervillositis (CHI) is a recurrent placental lesion where maternal macrophages infiltrate the intervillous space. Currently, its cause is unknown, though due to similarities to rejected allografts one hypothesis is that CHI represents maternal-fetal rejection. Here, virtual crossmatching was applied to healthy pregnancies and those with a history of CHI. Plasma anti-HLA antibodies were measured via Luminex, and percentage calculated reaction frequency (%cRF) determined. Antibodies were compared to fetal HLA to identify fetal specific antibodies (FSAs). Immunohistochemistry was used to identify placental C4d in untreated (index) CHI, subsequent pregnancies and healthy controls. There were no significant differences in C4d deposition between controls, index pregnancies with CHI and subsequent pregnancies, (control 0.75 [IQR 0-1.1] vs index 1.06 [0.3–1.6] vs subsequent pregnancies 1.13 [0.6–1.4]). Anti-HLA antibodies were present in slightly more controls than CHI (8/17 (47.1%) vs 6/15 (40.0%), respectively), but there was no significant difference in levels of sensitisation or %cRF. There was no difference in FSAs between controls and CHI cases (median 2 [IQR 0-3.8] vs 5 [1.0–6.0]). Though CHI shares some similarities with rejection, an antibody-mediated component could not be confirmed. These data suggest that CHI may not result from anti-HLA antibodies, but immunomodulatory treatment is an important confounding factor. Further investigation of antibodies independent of treatment is required to determine any possible role in the pathophysiology of CHI.
The application of 3D-printed auto-stable artificial vertebral body in ENBLOC resection and reconstruction of thoracolumbar metastases
Authors: Cao Yun , Yang Nan , Wang Shengbao , Wang Cong , He Qiang , Wu Qinfan , Zheng Yangyang 
Published: June 2023

AbstractBackground The nerve compression symptoms and spinal instability caused by spinal metastases seriously affect the patient's quality of life. A 3D-printed vertebral body is one of the optional methods for reconstruction of a bone defect after en-bloc resection of spinal tumors, with less postoperative complications because of its tailored shape and innermost porous structure inducing bone ingrowth. Objective To evaluate the efficacy of 3D-printed auto-stable artificial vertebrae in en-bloc resection and reconstruction of thoracolumbar metastases. Methods Between January 2019 and April 2021, patients who underwent en-bloc resection of thoracolumbar metastases based on the Weinstein-Boriani-Biagini surgical staging system were included in this study. The patients were divided into observation group (reconstructed with 3D-printed auto-stable vertebral body) and control group (treated with titanium cage and allograft bone). The patients were evaluated according to subsidence of implants, instrumentation-related complications, VAS score, and Frankel grading of spinal cord injury. Results The median follow-up was 21.8 (range 12 ~ 38) months. The customized 3D-printed artificial vertebral body was used in 10 patients, and the titanium cage was used in the other 10 patients. The operation time, intraoperative blood loss and postoperative drainage in the observation group were significantly lower than that in the control group(P < 0.05). The average implants subsidence of observation and control group at the final follow-up was 1.8 ± 2.1 mm and 5.2 ± 5.1 mm respectively(P < 0.05). The visual analogue scale (VAS) between the two groups at preoperative, 24 hours, 3 months and 1 year after operation was not statistically different (P < 0.05). No statistical significance was found in the improvements of spinal cord functions of the two groups. Conclusion The use of a 3D-printed auto-stable artificial vertebra for reconstruction after en-bloc resection of the thoracolumbar metastases may be a feasible and reliable option. The low incidence of prosthesis subsidence of 3D-printed prosthesis can provide good stability instantly.
Finishing of cellulosic fabrics based on SeNPs@triazole derivatives as anti- mosquito
Authors: Abdelhamid Ahmed , Kamel Omnia , Amr A. , Swelam Samira , El-Sayed Ahmed 
Published: June 2023

Abstract
As mosquito-borne diseases spread around the world, mosquito repellent textiles are in high demand as a disease-prevention tool for mosquito bites. In, this study selenium nanoparticles (SeNPs) conjugated by synthesized triazole compound 7-(3-methoxyphenyl)-8-methyl-5-(5-methylfuran-2-yl)pyrimido[5,4-e][1,2,4]-triazolo[1,5-a] pyrimidin-6(7H) one was used to modify 100%, cotton, 65/35%, cotton/PET, viscose and gauzy cotton fabrics, for use as mosquito repellent and morality. The synthesized triazole derivative and the nanoparticles were confirmed by different instrumental techniques. The presence of SeNPs@triazole derivative into fabrics surface was investigated using FT-IR, SEM and EDX. The treated fabric repellency to Culex pipiens mosquitoes showed stable 100% repellency for two hours then complete morality after one day, the samples of fabrics are more durable by ratio 100% after 9th washing but decrease from 10th washing.