bluuebunny/crossref_metadata_2025_split huggingface dataset:
Atributos da Atenção Primária à Saúde no Cuidado de Crianças em São Paulo/ SP
Authors: Silva Bruna Isabele Gomes da , Ayello Pietra Ribeiro , Monteiro Camila Nascimento , Mafra Ana Carolina  Cintra Nunes , Almeida Letícia Yamawaka de , Peres Neto João 
Published: March 2023

O objetivo foi avaliar os atributos da Atenção Primária à Saúde (APS) no cuidado das crianças em Unidades Básicas de Saúde (UBS). Estudo transversal com abordagem quantitativa, desenvolvido em 13 UBS no município de São Paulo/ SP, no ano de 2021. Para a avaliação dos atributos da APS foi utilizado o instrumento PCATool-Brasil para crianças. Foram avaliados os escores de cada atributo separadamente e também os escores geral e essencial. A amostra foi caracterizada a partir da média e desvio padrão, mínimo e máximo, mediada e quartis, para as variáveis quantitativas, e pelas frequências absolutas e relativas para as variáveis qualitativas. Participaram 242 crianças, com idade média de 3,33 anos e os escores essencial com valores de (6,41) e geral (6,20) respectivamente. Os resultados dos escores apresentaram-se baixos e, portanto, com necessidade de melhorias nos serviços da APS. Os resultados dos escores essencial e geral apresentados como baixos e, portanto, com necessidade de melhorias nos serviços da APS evidenciam à busca constante por avaliações dos mesmos, objetivando uma gestão qualificada e que traga uma maior resolutividade à APS.
A novel model extended from the Bouguer-Lambert-Beer law can describe the non-linear absorbance of potassium dichromate solutions and microalgae suspensions
Authors: Yeh Yen-Cheng , Haasdonk Bernard , Schmid-Staiger Ulrike , Stier Matthias , Tovar Günter E. M. 
Published: March 2023

Introduction: The Bouguer-Lambert-Beer law is widely used as the fundamental equation for quantification in absorption spectroscopy. However, deviations from the Bouguer-Lambert-Beer law have also been observed, such as chemical deviation and light scattering effect. While it has been proven and shown that the Bouguer-Lambert-Beer law is valid only under very restricted limitations, there are only a few alternatives of analytical models to this law. Based on the observation in the experiments, we propose a novel model to solve the problem of chemical deviation and light scattering effect.Methods: To test the proposed model, a systematic verification was conducted using potassium dichromate solutions and two types of microalgae suspensions with varying concentrations and path lengths.Results: Our proposed model demonstrated excellent performance, with a correlation coefficient (R2) exceeding 0.995 for all tested materials, significantly surpassing the Bouguer-Lambert-Beer law, which had an R2 as low as 0.94. Our results confirm that the absorbance of pure pigment solutions follows the Bouguer-Lambert-Beer law, while the microalgae suspensions do not due to the light scattering effect. We also show that this scattering effect leads to huge deviations for the commonly used linear scaling of the spectra, and we provide a better solution based on the proposed model.Discussion: This work provides a powerful tool for chemical analysis and especially for the quantification of microorganisms, such as the concentration of biomass or intracellular biomolecules. Not only the high accuracy but also the simplicity of the model makes it a practical alternative to the existing Bouguer-Lambert-Beer law.
Magnetic iron oxide nanoparticle-loaded hydrogels for photothermal therapy of cancer cells
Authors: Ji Yunfei , Wang Chunpu 
Published: March 2023

Introduction: Non-invasive photothermal therapy (PTT) is a competitive treatment for solid tumors, while the efficacy is largely dependent on the effective retention of photothermal converters in tumor tissues.Methods: Herein, the development of iron oxide (Fe3O4) nanoparticle-loaded alginate (ALG) hydrogel platform for PTT of colorectal cancer cells is reported. Fe3O4 nanoparticles synthesized via coprecipitation method after reaction of 30 min have a small size (61.3 nm) and more suitable surface potential, and can mediate PTT under near-infrared (NIR) laser irradiation. The premix of Fe3O4 nanoparticles and ALG hydrogel precursors can be gelatinized by Ca2+-mediated cross-linking to form this therapeutic hydrogel platform.Results: The formed Fe3O4 nanoparticles can be effectively taken up by CT26 cancer cells and induce the death of CT26 cells in vitro under NIR laser irradiation because of their excellent photothermal property. In addition, Fe3O4 nanoparticle-loaded ALG hydrogels show negligible cytotoxicity at the studied concentration range, but can significantly kill cancer cells after PTT effect.Conclusion: This ALG-based hydrogel platform provides a valuable reference for subsequent in vivo studies and other related studies on Fe3O4 nanoparticle-loaded hydrogels.
Biodegradable hollowed mesoporous SeO2 nanoplatform loaded with indocyanine green for simultaneous NIR II fluorescence imaging and synergistic breast carcinoma therapy
Authors: Peng Tingwei , Liu Qing , Song Hui , Zhang Conghui , Wang Xue , Ru Ping , Xu Tianzhao , Liu Xinghui 
Published: March 2023

Contrast agents in the second window of the near-infrared region (NIR II, 1000–1700 nm) have several advantages and indocyanine green (ICG), which emits NIR II fluorescence, is clinically approved and its use has been widely investigated for in vivo imaging, specifically for delineating tumor outlines; however, insufficient tumor targeting and rapid physiological metabolism of free ICG has substantially impeded its further clinical application. Here, we constructed novel hollowed mesoporous selenium oxide nanocarriers for precise ICG delivery. After surface modification with the active tumor targeting amino acid motif, RGD (hmSeO2@ICG-RGD), the nanocarriers were preferentially targeted toward tumor cells and subsequently degraded for ICG and Se-based nanogranule release under tumor tissue extracellular pH conditions (pH 6.5). The released ICG acted as an NIR II contrast agent, highlighting tumor tissue, after intravenous administration of hmSeO2@ICG-RGD into mammary tumor-bearing mice. Importantly, the photothermal effect of ICG improved reactive oxygen species production from SeO2 nanogranules, inducing oxidative therapy. The synergistic therapeutic effects of hyperthermia and increased oxidative stress on 808 nm laser exposure induced significant tumor cell killing. Thus, our nanoplatform can generate a high-performance diagnostic and therapeutic nanoagent that facilitates in vivo tumor outline discrimination and tumor ablation.
Clinical features and diagnostic value of metagenomic next -generation sequencing in five cases of non-HIV related Pneumocystis jirovecii pneumonia in children
Authors: Niu Jiechao , Wang Jiandong , Jia Peisheng , Zhang Mengjiao , Wei Erhu 
Published: March 2023

BackgroundPneumocystis jirovecii (PJ) is an opportunistic pathogenic fungus, and PJ pneumonia (PJP) is a commonly problem in HIV-positive patients. While PJP is not caused by HIV, it generally advances rapidly and can quickly lead to severe respiratory failure. To improve pediatricians’ understanding of the condition and aid early accurate diagnoses and therapy, we examined the clinical characteristics of five instances of non-HIV related PJP (NH-PJP) in children and the efficacy of metagenomic next-generation sequencing (mNGS) in its diagnosis.MethodsFrom January 2020 to June 2022, five children with NH-PJP were admitted to the PICU of the First Affiliated Hospital of Zhengzhou University. We retrospectively summarize the clinical presentation, previous histories, routine laboratory findings, treatment, outcome of regression, and results of mNGS in these five children.ResultsFive male children between the ages of 11 months and 14 years had an acute onset on NH-PJP, three of the children had chest tightness after activity, shortness of breath and paroxysmal dry cough, — and two had high fever and dry cough. All five of the children had several flocculent high-density pictures in both lungs at the beginning of the disease, and lung auscultation revealed coarse breath sounds in both lungs, one of which was accompanied by a modest quantity of dry rales. PJ nuclear sequences were found in one patient and four patients’ blood and alveolar lavage fluid. All five children were treated with Trimethoprim-sulfamethoxazole (TMP-SMX) in combination with Caspofungin and corresponding symptomatic treatment. Four patients were cured and one patient died.ConclusionChildren commonly encounter an initial exposure to NH-PJP, which manifests as a high fever, dry cough, chest discomfort, dyspnea that worsens over time, fast disease progression, and a high death rate. The clinical presentation of children with PJ infection should be taken into consideration along with the results for diagnose. mNGS has higher sensitivity and a shorter detection period compared to identification of PJP.
Supporting Ph.D. students' skills development: A three-stage doctoral program
Authors: Chaló Paulo , Huet Isabel , Nikoletou Dimitra , Pereira Anabela 
Published: March 2023

IntroductionSustainable development is increasingly recognized as a crucial component of education at all levels, including specialized academic work. As a result, the importance of Ph.D. training in producing highly skilled academics and professionals who can contribute to sustainable development has been acknowledged, leading to increased investments in doctoral training across Europe. However, the diversity in Ph.D. training across countries and universities suggests a lack of studies based on best practices for developing effective doctoral training programs.MethodsThis study aimed to contribute to this discussion by combining the perspectives of 31 Ph.D. supervisors and directors from Portugal and England regarding the best practices for Ph.D. training. Participants from different disciplines were interviewed using semi-structured face-to-face interviews, and the data were analyzed using inductive thematic analysis.ResultsThe study found that, despite coming from different countries, the participants' perceptions regarding the challenges and strategies for improving doctoral education were similar. Based on these findings, the study proposes a three-stage program for structuring doctoral training, consisting of initial intensive training, assessment of individual needs, and tailored training.DiscussionThe proposed program has the potential to be valuable in the development or restructuring of Ph.D. programs to address the primary needs of Ph.D. students, optimize institutional resources, and promote on-time completion by facilitating the timely development of necessary competencies for Ph.D. research. Furthermore, this approach could enhance the quality of Ph.D. education, broaden the spectrum and quality of graduates' competencies, and contribute to achieving sustainable development goals.
Study on hot air heating characteristics of greenhouse in cold region
Authors: Zhang Chenghu , Zou Dezhi , Huang Xinpeng , Wu Yaling 
Published: March 2023

Agriculture is the main industry in northeast China. As an important part of facility agriculture, agricultural greenhouses are playing a more and more important role. Due to geographical location, Northeast China is cold in winter, and the solar radiation energy is less, therefore, the large-span greenhouses commonly used in northeast China are prone to uneven temperature and insufficient heating. In this paper, in order to solve the phenomenon of uneven air flow field, humidity field and temperature field in greenhouse, based on AIRPAK simulation, four kinds of hot air heating return air modes, such as upper air supply and lower air return mode, lower air supply and upper air return mode, two-side air supply mode and left air supply and right air return mode, we explored the influence of temperature field, humidity field and air flow field in greenhouse. The results showed that the heat load of the greenhouse in winter is 161.51 kW, using two-side air supply mode is the best way. The air supply wind speed is 1.6 m/s, and the return wind speed is 3.1 m/s. The environment of the greenhouse can be maintained at about 15°C day and night, and the relative humidity is relatively uniform. In the crop growing area, the wind speed is between 0 m/s and 0.25 m/s. According to this research, suitable crops can be selected for reasonable planting and distribution in the greenhouse to maximize productivity.
Maternal genetic polymorphisms in the major mitotic checkpoint genes MAD1L1 and MAD2L1 associated with the risk of survival in abnormal chromosomal fetuses
Authors: Chan Ying , Liu Yize , Kong Yamin , Xu Weiming , Zeng Xiaohong , Li Haichun , Guo Yan , Tang Xinhua , Zhang Jinman , Zhu Baosheng 
Published: March 2023

Background: The genetic etiology of fetal chromosome abnormalities remains unknown, which brings about an enormous burden for patients, families, and society. The spindle assembly checkpoint (SAC) controls the normal procedure of chromosome disjunction and may take part in the process.Objective: The aim of this study was to explore the association between polymorphisms of MAD1L1 rs1801368 and MAD2L1 rs1283639804, involved in SAC and fetal chromosome abnormalities.Methods: The case–control study collected 563 cases and 813 health controls to test the genotypes of MAD1L1 rs1801368 and MAD2L1 rs1283639804 polymorphisms by polymerase chain reaction–restrictive fragment length polymorphism methods (PCR-RFLP).Results:MAD1L1 rs1801368 polymorphism was associated with fetal chromosome abnormalities alone or combined to lower homocysteine (HCY) levels (alone: dominant: OR: 1.75, 95%CI: 1.19–2.57, and p = 0.005; CT vs. CC: OR = 0.73, 95%CI: 0.57–0.94, and p = 0.016; lower HCY: C vs. T: OR = 0.74, 95%CI: 0.57–0.95, and p = 0.02; dominant: OR = 1.75, 95%CI: 0.79–1.92, and p = 0.005). No significant differences were found in other genetic models or subgroups (p &gt; 0.05, respectively). MAD2L1 rs1283639804 polymorphism revealed a sole genotype in the studied population. HCY is significantly associated with fetal chromosome abnormalities in younger groups (OR: 1.78, 95%CI: 1.28–2.47, and p = 0.001).Conclusion: The results implied that the polymorphism of MAD1L1 rs1801368 may become the susceptibility factor to fetal chromosome abnormalities alone or combined to lower HCY levels but not to MAD2L1 rs1283639804 polymorphism. In addition, HCY significantly affects fetal chromosomal abnormalities in younger women.
Pan-cancer analysis identifies PD-L2 as a tumor promotor in the tumor microenvironment
Authors: Lv Jingfang , Jiang Zheng , Yuan Junhu , Zhuang Meng , Guan Xu , Liu Hengchang , Yin Yefeng , Ma Yiming , Liu Zheng , Wang Hongying , Wang Xishan 
Published: March 2023

BackgroundProgrammed cell death protein 1 (PD-1) receptor has two ligands,programmed death-ligand 1 (PD-L1) and PD-L2. When compared with PD-L1, PD-L2 has not received much attention, and its role remains unclear.MethodsThe expression profiles of pdcd1lg2 (PD-L2-encoding gene) mRNA and PD-L2 protein were analyzed using TCGA, ICGC, and HPA databases. Kaplan-Meier and Cox regression analyses were used to assess the prognostic significance of PD-L2. We used GSEA, Spearman’s correlation analysis and PPI network to explore the biological functions of PD-L2. PD-L2-associated immune cell infiltration was evaluated using the ESTIMATE algorithm and TIMER 2.0. The expressions of PD-L2 in tumor-associated macrophages (TAMs) in human colon cancer samples, and in mice in an immunocompetent syngeneic setting were verified using scRNA-seq datasets, multiplex immunofluorescence staining, and flow cytometry. After fluorescence-activated cell sorting, flow cytometry and qRT-PCR and transwell and colony formation assays were used to evaluate the phenotype and functions of PD-L2+TAMs. Immune checkpoint inhibitors (ICIs) therapy prediction analysis was performed using TIDE and TISMO. Last, a series of targeted small-molecule drugs with promising therapeutic effects were predicted using the GSCA platform.ResultsPD-L2 was expressed in all the common human cancer types and deteriorated outcomes in multiple cancers. PPI network and Spearman’s correlation analysis revealed that PD-L2 was closely associated with many immune molecules. Moreover, both GSEA results of KEGG pathways and GSEA results for Reactome analysis indicated that PD-L2 expression played an important role in cancer immune response. Further analysis showed that PD-L2 expression was strongly associated with the infiltration of immune cells in tumor tissue in almost all cancer types, among which macrophages were the most positively associated with PD-L2 in colon cancer. According to the results mentioned above, we verified the expression of PD-L2 in TAMs in colon cancer and found that PD-L2+TAMs population was not static. Additionally, PD-L2+TAMs exhibited protumor M2 phenotype and increased the migration, invasion, and proliferative capacity of colon cancer cells. Furthermore, PD-L2 had a substantial predictive value for ICIs therapy cohorts.ConclusionPD-L2 in the TME, especially expressed on TAMs, could be applied as a potential therapeutic target.
Perivascular adipose tissue promotes vascular dysfunction in murine lupus
Authors: Shi Hong , Goo Brandee , Kim David , Kress Taylor C. , Ogbi Mourad , Mintz James , Wu Hanping , Belin de Chantemèle Eric J. , Stepp David , Long Xiaochun , Guha Avirup , Lee Richard , Carbone Laura , Annex Brian H. , Hui David Y. , Kim Ha Won , Weintraub Neal L. 
Published: March 2023

IntroductionPatients with systemic lupus erythematosus (SLE) are at elevated risk for Q10 cardiovascular disease (CVD) due to accelerated atherosclerosis. Compared to heathy control subjects, lupus patients have higher volumes and densities of thoracic aortic perivascular adipose tissue (PVAT), which independently associates with vascular calcification, a marker of subclinical atherosclerosis. However, the biological and functional role of PVAT in SLE has not been directly investigated.MethodsUsing mouse models of lupus, we studied the phenotype and function of PVAT, and the mechanisms linking PVAT and vascular dysfunction in lupus disease. Results and discussionLupus mice were hypermetabolic and exhibited partial lipodystrophy, with sparing of thoracic aortic PVAT. Using wire myography, we found that mice with active lupus exhibited impaired endothelium-dependent relaxation of thoracic aorta, which was further exacerbated in the presence of thoracic aortic PVAT. Interestingly, PVAT from lupus mice exhibited phenotypic switching, as evidenced by “whitening” and hypertrophy of perivascular adipocytes along with immune cell infiltration, in association with adventitial hyperplasia. In addition, expression of UCP1, a brown/beige adipose marker, was dramatically decreased, while CD45-positive leukocyte infiltration was increased, in PVAT from lupus mice. Furthermore, PVAT from lupus mice exhibited a marked decrease in adipogenic gene expression, concomitant with increased pro-inflammatory adipocytokine and leukocyte marker expression. Taken together, these results suggest that dysfunctional, inflamed PVAT may contribute to vascular disease in lupus.
Analysis of potential immune-related genes involved in the pathogenesis of ischemia-reperfusion injury following liver transplantation
Authors: Guo Jiayu , Han Shangting , Chen Qi , Wang Tianyu , Yu Bo , Zhou Jiangqiao , Qiu Tao 
Published: March 2023

BackgroundHepatic ischemia-reperfusion (I/R) injury is an unavoidable pathological process that occurs after liver transplantation. However, the immune-related molecular mechanism still remains unclear. This study aims to further explore the biological mechanisms of immune-related genes in hepatic I/R injury.MethodsGene microarray data was downloaded from the Gene Expression Omnibus (GEO) expression profile database and the differentially expressed genes (DEGs) were taken for intersection. After identifying common DEGs, functional annotation, protein-protein interaction (PPI) network, and modular construction were performed. The immune-related hub genes were obtained, which their upstream transcription factors and non-RNAs were predicted. Validation of the hub genes expression and immune infiltration were performed in a mouse model of hepatic I/R injury.ResultsA total of 71 common DEGs were obtained from three datasets (GSE12720, GSE14951, GSE15480). The GO and KEGG enrichment analysis results indicated that immune and inflammatory response played an important role in hepatic I/R injury. Finally, 9 immune-related hub genes were identified by intersecting cytoHubba with immune-related genes, including SOCS3, JUND, CCL4, NFKBIA, CXCL8, ICAM1, IRF1, TNFAIP3, and JUN.ConclusionOur study revealed the importance of the immune and inflammatory response in I/R injury following liver transplantation and provided new insights into the therapeutic of hepatic I/R injury.
Knowledge domain and emerging trends in HIV pre-exposure prophylaxis: A visualization analysis via CiteSpace
Authors: Chen Xi , Lai Yu 
Published: March 2023

BackgroundAs an effective strategy that reduces transmission among people at high risk of human immunodeficiency virus (HIV), pre-exposure prophylaxis (PrEP) has revolutionized HIV prevention. Our study aims to provide a reference for the development of relevant research and the formulation of prevention and control policies for HIV.MethodsThrough CiteSpace software, this study aims to present a comprehensive overview of the HIV PrEP knowledge structure, hotspots, and frontiers. We searched the Web of Science Core Collection for studies published between 2012 and 2022 related to HIV PrEP, and 3,243 papers remained after selection.ResultsThe number of HIV PrEP publications has increased over the past few years. A close integration and exchange of HIV PrEP research findings has taken place between countries and authors. Major ongoing research trends include long-term injection PrEP, the impact of chlamydia on HIV PrEP, and individual awareness of and attitudes toward HIV PrEP. Thus, more attention should be paid to innovations and breakthroughs in drugs, the factors that affect HIV transmission and susceptibility, and the future promotion of public acceptance of HIV PrEP.ConclusionThis study offers a systematic, objective, and comprehensive analysis of the related articles. It will assist scholars in understanding the dynamic evolution of HIV PrEP research and identifying future research areas to better advance the development of the field.
Lactiplantibacillus plantarum N-1 improves autism-like behavior and gut microbiota in mouse
Authors: Qiu Zhongqing , Luo Dongmei , Yin Heng , Chen Yajun , Zhou Zhiwei , Zhang Jing , Zhang Linzhu , Xia Jinrong , Xie Jiang , Sun Qun , Xu Wenming 
Published: March 2023

IntroductionThe gut-brain axis has been widely recognized in autism spectrum disorder (ASD), and probiotics are considered to potentially benefit the rescuing of autism-like behaviors. As a probiotic strain, Lactiplantibacillus plantarumN-1(LPN-1) was utilized to investigate its effects on gut microbiota and autism-like behaviors in ASD mice constructed by maternal immune activation (MIA).MethodsAdult offspring of MIA mice were given LPN-1 at the dosage of 2  ×  109  CFU/g for 4  weeks before subject to the behavior and gut microbiota evaluation.ResultsThe behavioral tests showed that LPN-1 intervention was able to rescue autism-like behaviors in mice, including anxiety and depression. In which the LPN-1 treatment group increased the time spent interacting with strangers in the three-chamber test, their activity time and distance in the central area increased in the open field test, and their immobility time decreased when hanging their tails. Moreover, the supplementation of LPN-1 reversed the intestinal flora structure of ASD mice by enhancing the relative abundance of the pivotal microorganisms of Allobaculum and Oscillospira, while reducing those harmful ones like Sutterella at the genus level.DiscussionThese results suggested that LPN-1 supplementation may improve autism-like behaviors, possibly via regulating the gut microbiota.