bluuebunny/crossref_metadata_2025_split huggingface dataset:
Lifestyle Factors and Laboratory Sperm Processing Techniques Are Correlated With Sperm Dna Fragmentation Index, Oxidative Stress Adducts, and High Dna Stainability
Authors: Broussard Alicia Lennea , Leader Benjamin , Russell Helena , Beydoun Hind , Colver Robert , Reuter Laura , Bopp Bradford , Will Matthew , Will Erica Anspach , Adaniya Glen 
Published: April 2023

Abstract
Purpose To determine correlation between lifestyle risk factors and sperm quality.Methods Patients (n = 133) who consented for the study completed a lifestyle questionnaire. An aliquot of sperm was frozen at three different timepoints. Preparation methods for 30 semen analysis were compared: ZyMōt Sperm Separation Device (DxNow), Isolate gradient (Irvine), SpermGrad gradient (Vitrolife), and each gradient was followed by swim-up (SU), Isolate + SU and Spermgrad + SU. All samples were analyzed using the Sperm DNA Fragmentation Assay (acridine orange/flow cytometry SDFA™). Analysis included DNA fragmentation index (DFI), oxidative stress adducts (OSA) and high DNA stainability (HDS). Statistical analysis was performed using JMP (SAS 2018) and P < 0.05 was considered statistically significant.Results The neat DFI was not correlated with age, morphology, or oligospermia (< 20 million/mL). Men that consumed alcohol daily trended towards a higher DFI than those that drank multiple times per week and significantly higher than those who never drink (p = 0.0608 and p = 0.0290, respectively), but interestingly not those who drank rarely. DFI was also positively correlated with OSA and HDS in the neat and processed sample (INSEM). The DFI of the INSEM sperm sample was positively correlated with age, poor morphology, and oligospermia (p = 0.0208, p < 0.0001, p = 0.0006, respectively). There was no correlation with BMI or smoking status for neat or processed sperm health. The separation device effectively improved the DFI, OSA, and HDS compared to other methodsConclusion Lifestyle factors and preparation method is correlated with sperm quality.
METTL16 Mediated LncRNA TIALD m6A Modification contributes to hepatocellular carcinoma metastasis via inducing AURKA lysosomal degradation
Authors: Zhao Bixing , Wang Yingchao , Zhong Yue , Zheng Xiaoyuan , Cheng Niangmei , Yang Yong , Yang Ye , Wang Fei , Zhuang Qiuyu , Huang Yao , Guo Wuhua , Liao Naishun , Yang Xiao-Yu , Liu Xiao-Long 
Published: April 2023

Abstract

The N6-methyladenosine (m6A) RNA methyltransferase METTL16 is an emerging player in RNA modification landscape and responsible for the deposition of m6A in a few transcripts. AURKA (aurora kinase A) has been confirmed as an oncogene in cancer development including hepatocellular carcinoma (HCC). Nevertheless, it remains unclear whether METTL16 mediated m6A modification of lncRNAs can regulate AURKA activation in cancer progression. Here we aimed to investigate the functional links between lncRNAs and the m6A modification in AURKA signaling and HCC progression. Here we show that LncRNA TIALD (transcript that induced AURKA Lysosomal degradation) was down-regulated in HCC tissues by METTL16 mediated m6A methylation to facilitate its RNA degradation, and correlates with poor prognosis. Functional assays reveal that TIALD inhibits HCC metastasis both in vitro and in vivo. Mechanistically, TIALD directly interacts with AURKA and facilitate its degradation through the lysosomal pathway to inhibited EMT and metastasis of HCC. AURKA’s specific inhibitor alisertib exerts effective therapeutic effect on liver cancer with low TIALD expression, which mightprovide a new insight into HCC therapy. Our study uncovers a negative functional loop of METTL16-TIALD-AURKA axis, and identifies a new mechanism for METTL16 mediated m6A-induced decay of TIALD on AURKA signaling in HCC progression, which may provide potential prognostic and therapeutic targets for HCC.
Genotoxic effect of heavy metals on Nile tilapia (Oreochromis niloticus) species in an urban river in northeastern Brazil
Authors: Silva Jordany G. , Chagas Cristiano A. , Souza Talita G. S. , Araújo Marlyete C. , Araujo Livia C. A. , Santos André M. M. , Sá Rafael A. Q. C. , Alves Rayanna B. O. , Rodrigues Rosner H. A. , Silva Hernande P. , Bezerra Ranilson S. , Oliveira Maria Betania M. 
Published: April 2023

Abstract
Chemical pollutants represent a leading problem for aquatic ecosystems, as they can induce genetic, biochemical, and physiological changes in the species of these ecosystems, thus compromising their adaptability and survival. The Capibaribe River runs through the state of Pernambuco, located in northeastern Brazil, and passes through areas of agricultural cultivation, densely populated cities, and industrial centers, primarily textiles. Despite its importance, few ecotoxicological studies have been conducted on its environment and knowledge about pollution patterns and their effects on its biota is scarce. The objective of this study was to evaluate the water quality and the damage caused by pollutants on the DNA specimens of Nile tilapia (Oreochromis niloticus) obtained from seven strategic points of Capibaribe. Tilapia specimens and water were collected during the rainy and dry seasons from 2015 to 2017. The following characteristics were analyzed: physicochemical (six), metal concentration (seven), local pluviosity, micronuclei and comet assay. The physicochemical and heavy metal analyses were exploratory, whereas the ecotoxicological analyses were hypothetical. To verify this hypothesis, we compared the groups of fish collected to the results of the micronuclei test and comet assay and created a Structural Equation Model (SEM) to determine how the micronuclei variables, damage index, pluviosity, and concentration of each metal were related. Our results demonstrated that the highest values for markers of genetic damage were detected at points with the highest heavy metal concentrations, especially iron, zinc, manganese, chromium, and cadmium. The SEM demonstrated that metals can explain the findings of the genotoxicity markers. Moreover, other pollutants, such as pesticides, should be considered, particularly in places where the river passes through rural areas. The results presented here demonstrate that the Capibaribe River has different degrees of contamination and confirm our hypothesis.
Assessing Dynamical Resilience Indicators in Older Adults: A Prospective Feasibility Cohort Study
Authors: Kolk Daisy , MacNeil-Vroomen Janet L. , Melis René J.F. , Ridderikhof Milan L. , Buurman Bianca M. 
Published: April 2023

Abstract
Purpose: Measuring dynamical resilience indicators based on time series data may improve the prediction of health deterioration in older adults after hospital discharge. We examined the feasibility of an intensive prospective cohort study examining dynamical resilience indicators based on time series data of symptoms and physical activity in acutely ill older adults who visited the Emergency Department (ED).
Methods: Prospective cohort study with time series data from symptom questionnaires and activity trackers. Thirty older adults (aged 75.9 ± 5.5 years, 37% female) who were discharged from the ED of a tertiary hospital in the Netherlands. We monitored self-reported symptoms using a daily online questionnaire, and physical activity using an activity tracker for 30 days. Descriptive data on participant eligibility and adherence to and acceptability of the assessments were collected.
Results: Of 134 older patients visiting the ED, 109/134 (81%) were eligible for inclusion and 30/109 (28%) were included. Twenty-eight (93%) of the included participants completed follow-up. Regarding the adherence rate, 78% of participants filled in the questionnaire and 80% wore the activity tracker. Three (10%) participants completed fewer than three questionnaires. Most participants rated the measurements as acceptable and seven (23%) participants experienced an adverse outcome in the 30 days after discharge.
Conclusion: An intensive prospective cohort study examining dynamical resilience indicators in older adults is feasible. The quality of the collected data was sufficient, some adjustments to the measurement protocol are recommended. This study is an important first step to study resilience in older adults.
Development and validation of a novel nomogram to predict the impact of the polymorphism of the ICAM-1 gene on the prognosis of ischemic cardiomyopathy
Authors: Naman Tuersunjiang , Abuduhalike Refukaiti , Abudouwayiti Aihaidan , Sun Juan , Mahemuti Ailiman 
Published: April 2023

Abstract
Object:
The current study investigated the association between polymorphisms of the ICAM-1 gene and prognosis of Ischemic cardiomyopathy(ICM), and developed a prognostic nomogram for ICM on the basis of ICAM-1 gene variants.
Method:
The current study included totally 252 patients with ICM. In addition, PCR-RFLP (polymerase chain reaction-restriction fragment length polymorphism) was used to genotype SNPs in the ICAM-1 gene in the patients. Later, the nomogram model was built by combining clinical data with ICAM-1 gene variants. This study used the least absolute shrinkage and selection operator (LASSO) regression model to optimize feature selection into an ICM prognostic model. Furthermore, multivariate Cox-regression was applied to build the prognostic model, which included clinical and gene features chosen by the LASSO regression model. Following that, the receiver operating characteristic (ROC) curve, C-index, calibration plot analyses and decision curve analysis (DCA) were carried out to evaluate the discrimination abilitiy, consistency, and clinical utility of the prognostic model, and the bootstrap method was adopted for internal validation.
Result:
predicting factors rs112872667, treating by PCI or CABG, ventricular arrhythmia, left ventricular end-diastolic diameter (LVDD), use of β-blockers, systolic blood pressure (SBP), heart rate (HR), and serum sodium were incorporated into the prognostic nomogram. The constructed nomogram performed well in discrimination ability, as observed by the time-dependent C-index. Furthermore, as shown by calibration curves, our nomogram’s predicted probabilities were highly consistent with measured values. With threshold probabilities, DCA suggested that our nomogram could be useful in the clinic.
Conclusion:
rs112872667 mutation (from CC genotype toCT or TTgenotype) is a protective factor for ICM patients to have a higher survival probability; ICM patients with the mutant genotype (CT or TT) have a lower probability of cardiogenic death than those with the wild genotype (CC).
Germline PTEN genotype-dependent phenotypic divergence during the early neural developmental process of forebrain organoids
Authors: Eng Charis , Kang Shin-Chung , Sarn Nicholas , Venegas Juan , Hitomi Masahiro 
Published: April 2023

Abstract

PTENgermline mutations account for ~ 1% of all autism spectrum disorder (ASD) cases, as well as ~ 17% of ASD patients with macrocephaly, making it one of the top ASD associated risk genes. Individuals with germlinePTENmutations receive the molecular diagnosis of PTEN Hamartoma Tumor Syndrome (PHTS), an inherited cancer predisposition syndrome, about 20–23% of whom are diagnosed with ASD. We generated forebrain organoid cultures from gene-edited isogenic human induced pluripotent stem cells (hiPSCs) harboring aPTENG132D(ASD) orPTENM134R(cancer) mutant allele to model how these mutants interrupt neurodevelopmental processrf. Here, we show that thePTENG132Dallele disrupts early neuroectoderm formation during the first several days of organoid generation. While organoids generated fromPTENM134RhiPSCs remained morphologically similar to wild-type organoids during this early stage in development, we observed disrupted neuronal differentiation, radial glia positioning, and cortical layering in bothPTENmutant organoids at the later stage of 72 + days of development. Perifosine, an AKT inhibitor, reduced over activated AKT and partially corrected the abnormalities in cellular organization observed inPTENG132Dorganoids. Single cell RNAseq analyses on early-stage organoids revealed that genes related to neural cell fate was least enriched inPTENG132Dmutant organoids, and AKT inhibition was capable of upregulating gene signatures related to neuronal cell fate and CNS maturation pathways. These findings demonstrate that differentPTENmissense mutations can have profound impact on neurodevelopment at diverse stages which in turn may predispose PHTS individuals to ASD. Further study will shed light on the way to mitigate pathological impact ofPTENmutants on neurodevelopment by stage-specific manipulation of downstream PTEN signaling components.
A case of retroperitoneal Doege-Potter syndrome with continuous intraoperative blood glucose monitoring
Authors: Nagasaka Hirotaka , Suzuki Takahisa , Kondo Takuya , Koizumi Mitsuyuki , Terao Hideyuki , Murohashi Yuko , Okubo Yoichiro , Yokose Tomoyuki , Kishida Takeshi 
Published: April 2023

Abstract
Background:
Doege-Potter syndrome presents as solitary fibrous tumors and non-islet cell tumor hypoglycemia (NICTH). Here, we report a case of Doege-Potter syndrome in which retroperitoneal tumor resection was performed with continuous intraoperative blood glucose monitoring.
Case Presentation:
The patient was a 37-year-old healthy man with no relevant medical history. They were brought to the emergency room because of defecation difficulties and incontinence caused by hypoglycemia, and a tumor measuring 10 × 12 × 9 cm was found in the right kidney. High-molecular-weight insulin-like growth factor-II (IGF-II) was detected in the blood, indicating an IGF-II-producing tumor with NICTH. The patient underwent tumor resection, and the pathological results indicated a solitary fibrous tumor of retroperitoneal origin. Thus, retroperitoneal primary Doege-Potter syndrome was diagnosed. Continuous blood glucose monitoring was performed intraoperatively, and no hypoglycemic attacks occurred; insulin secretory abnormalities improved immediately after surgery. Previous studies suggest that IGF-II is metabolized in a few hours. Therefore, serum IGF-II levels were analyzed one week and one month postoperatively; the levels were within the normal range at both time points. Two years have passed since the surgery without recurrence.
Conclusions:
Doege-Potter syndrome of retroperitoneal primary origin is rare. Furthermore, continuous intraoperative blood glucose monitoring was performed during surgery; thus, we report blood glucose level trends for the first time. This case highlights that this type of surgery can be performed safely without special blood glucose adjustments and that insulin secretion resumes soon after surgery.
Absorbable protein stent for vasovasostomy in a rat model
Authors: Zhang Hui , Tu Xuchong , Chen Xiaohong , Liu Zhuojie , Feng Jiarong , Zhang Yan 
Published: April 2023

Abstract
PURPOSE: This study aimed to evaluate the safety and efficacy of a rapidly absorbable protein stent during microsurgical vasovasostomy.MATERIALS AND METHODS: Fifty-four rats were randomly divided into control, conventional, and stent groups. The left vas deferens was ligated in the control group and cut with double-layer suturing in the conventional group. The stent group received full-layer suturing with protein stent implantation. Patency rate, sperm quality, and reproductive hormones were evaluated up to 8 weeks. Pathological changes were determined by assessment.RESULTS: Patency rates were similar for the stent and conventional groups (8th week) (p > 0.05). Operation time was lower for the stent group than for the conventional group (p < 0.05). The stent group did not differ significantly from the control or conventional groups in terms of effects on sperm quantity, motility, deformity rate, FSH level, LH level, and testosterone level (p > 0.05).CONCLUSIONS: This study provides a foundation for future studies that may further explore the potential of protein stents in vasovasostomy and other surgical procedures.
A Text Mining Approach to Identify and Analyse Freight Logistics Service Demands
Authors: Ruoyu Wu , Chunfu Shao 
Published: April 2023

Abstract
The pandemic COVID-19 has caused significant impacts on the freight supply chain, based on the data from the traffic service hotline, the time and space characteristics of calls from drivers were described, and a 2-stage LDA model was built to quickly identify the demand for freight logistics services. The results showed as follows: 1) Hotline records fluctuated significantly with pandemic policy changes; 2) The top 4 demands for freight logistics services are road conditions consultation, applying the permit for vehicles carrying essential goods and materials, refueling and gas service consultation, and traffic control complaint; 3) 10 expressways, including G45, G10 and G59, as well as the three cities of Hohhot, Ordos and Wuhai, are under great pressure to ensure the stability of supply chains; 4) Information disclosure mechanism, driver service guarantee and hotline operation mechanism need to be improved. The research results verify the effectiveness of text mining technology in describing and identifying transport service demands. The proposed technical framework promotes the transformation of transport service from extensive governance to precise governance, which can provide a reference for promoting the stability of the logistics supply chain and exploring a participative approach to managing the industry.
A New Treatment Solution of Interval Nonlinear Programming Problems: A Case Study of Green Fuel Production
Authors: Elsisy M. A. , Hammad D. A. , Abdel-Aty Marwa M , El-Shorbagy M. A. 
Published: April 2023

Abstract
Green fuel is growing in popularity in recent years. Bio-reactive waste converted to green fuel through anaerobic digestion technology. The performance of biogas unit has been optimized and formulated as interval programming problems as function of inlet feed rate, retention time fermentation temperature and pH. A new treatment for solving the interval nonlinear programming problem (INPP) is discussed. All the intervals in the INPP are replaced by new variables. This the modified nonlinear programming problem (MIPP). We presented three hybrid evolutionary algorithms (EAs) which are chaotic genetic algorithm (CGA), chaotic particle swarm optimization (CPSO) and chaotic firefly algorithm (CFA) to solve MIPP. The Karush–Kuhn–Tucker (KKT) conditions for MIPP are gotten. These equations are solved as algebraic equations. Its solutions may be represented as a function of new variables to get the stability set of first kind. The staring points in EAs is gotten by the Newton method. Finally, the comparison between the stability set of first kind, CGA, CPSO and CFA are presented with discussion. An empirical optimization model of biogas production has been constructed with accuracy of 90%.
Decreased expression of ribosomal protein Rpl3 contributes to behavioral deficits caused by Shank3 deficiency
Authors: Verpelli Chiara , Giona Federica , Beretta Stefania , Zippo Antonio , Vicidomini Cinzia , Ponzoni Luisa , Sala Mariaelvina , Jones Carrie , Conn P. , Böckers Tobias , Sala Carlo 
Published: April 2023

Abstract
Mutations or deletions in the SHANK3 gene have been identified in up to 1% of autism spectrum disorder cases and are considered the main cause of neuropsychiatric symptoms of Phelan McDermid syndrome (PMS). While in the absence of Shank3, synaptic dysfunctions have been extensively described, other mechanisms through which Shank3 could regulate neuronal functions have not been clearly elucidated. Here, we reported that the ribosomal protein Rpl3 was downregulated in cortex and striatum of Shank3 KO mice and in neurons differentiated from hiPSCs derived from a PMS patient. Rpl3 is essential for ribosomal biogenesis and function and its reduced expression resulted in impaired protein synthesis in Shank3 KO mice that can be rescued by restoring its expression. Furthermore, we showed that chronic treatment with VU0409551, a potent and selective mGlu5 positive allosteric modulator, rescued Rpl3 expression and the resulting reduced protein synthesis, leading to a long-lasting improvement of behavioral deficits in Shank3 KO mice. Altogether, we suggest a new role for Shank3 in modulating ribosomal function and protein synthesis, and that restoring protein synthesis could be a strategy to correct Shank3 KO related behavioral phenotypes.
Application of modified neck drainage in the management of cervical anastomotic fistula in esophageal cancer patients
Authors: Wu Song , Zhang Zhe , Qin Zhonghua 
Published: April 2023

Abstract
Background Anastomotic fistula is a rare but life-threatening complication of esophageal cancer, and its treatment remains challenging.We aimed to evaluate the clinical efficacy of modified cervical drainage in the management of cervical anastomotic fistula in patients after esophageal cancer surgery.Methods From June 2017 to December 2021, 25 esophageal cancer patients who developed cervical anastomotic fistula were enrolled in the study. Among them, 14 patients were treated with modified cervical drainage, which served as group A, while the remaining 11 patients underwent open incision and drainage, which served as group B. The treatment outcomes of the two groups were compared and analyzed.Results The group A had a significantly shorter healing time(12.93 ± 3.93 vs 19.73 ± 5.29 ), fewer dressing changes(14.93 ± 3.93 vs 37.91 ± 9.77), shorter hospital stay(22.36 ± 4.39 vs 29.00 ± 5.79), and lower hospitalization costs(11.02 ± 1.73 vs 13.50 ± 2.43) compared to group B (P < 0.05). The incidence of complications(0 vs 9.1%) in the neck was not significantly different between the two groups (P > 0.05). All patients recovered smoothly and gradually resumed normal diet after discharge. They did not experience symptoms such as fever, difficulty in eating, or bucking.Conclusions Modified cervical drainage is an effective treatment for cervical anastomotic fistula in esophageal cancer patients, and when combined with nutritional and nursing support, can promote patient recovery.
Catheter-based renal sympathetic denervation may not be appropriate for the treatment of hypertension caused by primary aldosteronism: a case report
Authors: Wang Li , Li Zhuqing , Li Chao , Lu Chengzhi 
Published: April 2023

Abstract
Previous studies on the treatment of hypertension with RDN were all aimed at patients with essential hypertension, while there were few studies on secondary hypertension, especially primary aldosteronism. Whether RDN can be used to treat hypertension due to primary aldosteronism remains a subject of much debate. Here, we present a 54-year-old man with a history of resistant hypertension spanning more than 10 years; secondary hypertension was ruled out 6 years ago, and he underwent renal sympathetic denervation(RDN) in 2016. The patient's blood pressure had been controllable after half a year of RDN. After six months, the patient's blood pressure began to rise gradually and he began to increase the type of antihypertensive medication. The patient was diagnosed with aldosteronoma 1 year ago and underwent laparoscopic adrenalectomy. After that, the patient's blood pressure controlled well.