bluuebunny/crossref_metadata_2025_split huggingface dataset:
Comparison of Endocrinologists’ Physical Examination Documentation for In-person vs Video Telehealth Diabetes Visits
Authors: Arao Kevin A , Fincke B Graeme , Zupa Margaret F , Vimalananda Varsha G 
Published: June 2023

Abstract

Objective
Outpatient diabetes mellitus (DM) care over video telehealth (TH) requires modifications to how endocrinologists complete physical examinations (PEs). But there is little guidance on what PE components to include, which may incur wide variation in practice. We compared endocrinologists’ documentation of DM PE components for in-person (IP) vs TH visits.

Methods
Retrospective chart review of 200 notes for new patients with DM from 10 endocrinologists (10 IP and 10 TH visits each) in the Veterans Health Administration between April 1, 2020, and April 1, 2022. Notes were scored from 0 to 10 based on documentation of 10 standard PE components. We compared mean PE scores for IP vs TH across all clinicians using mixed effects models. Independent samples t-tests were used to compare both mean PE scores within clinician and mean scores for each PE component across clinicians for IP vs TH. We described virtual care-specific and foot assessment techniques.

Results
The overall mean (SE) PE score was higher for IP vs TH (8.3 [0.5] vs 2.2 [0.5]; P &lt; .001). Every endocrinologist had higher PE scores for IP vs TH. Every PE component was more commonly documented for IP vs TH. Virtual care-specific techniques and foot assessment were rare.

Conclusions
Our study quantifies the degree to which Pes for TH were attenuated among a sample of endocrinologists, raising a flag that process improvements and research are needed for virtual Pes. Organizational support and training could help increase PE completion via TH. Research should examine reliability and accuracy of virtual PE, its value to clinical decision-making, and its impact on clinical outcomes.
Intermittent Fasting Sustainably Improves Glucose Tolerance in Normal Weight Male Mice Through Histone Hyperacetylation
Authors: Endo Sho , Uto Asuka , Miyashita Kazutoshi , Sato Masaaki , Inoue Hiroyuki , Fujii Kentaro , Hagiwara Aika , Ryuzaki Masaki , Oshida Takuma , Kinouchi Kenichiro , Itoh Hiroshi 
Published: June 2023

Abstract
To explore the mechanism by which intermittent fasting (IF) exerts prolonged effects after discontinuation, we examined mice that had been subjected to 4 cycles of fasting for 72 hours and ad libitum feeding for 96 hours per week (72hIF), followed by 4 weeks of ad libitum feeding, focusing on expression of genes for lipid metabolism in the skeletal muscle and histone acetylation in the promoter region. The 72hIF regimen resulted in metabolic remodeling, characterized by enhanced lipid utilization and mitochondrial activation in the muscle. This long-term IF (72hIF) caused stronger metabolic effects than alternate day fasting (24hIF) wherein fasting and refeeding are repeated every 24 hours. Upregulation of lipid oxidation genes and an increase in oxygen utilization were sustained even at 4 weeks after discontinuation of 72hIF, associated with histone hyperacetylation of the promoter region of uncoupling protein 3 (Ucp3) and carnitine palmitoyl transferase 1b (Cpt1b) genes. An increase in leucine owing to fasting-induced muscle degradation was suggested to lead to the histone acetylation. These findings support the previously unappreciated notion that sustainable promotion of histone acetylation in lipid oxidation genes of the muscle and adipose tissues during and after IF may contribute to sustained metabolic effects of IF.
Prevalence of Endocrine Manifestations and GIST in 108 Systematically Screened Patients With Neurofibromatosis Type 1
Authors: Dupuis Hippolyte , Chevalier Benjamin , Cardot-Bauters Catherine , Jannin Arnaud , Do Cao Christine , Ladsous Miriam , Cortet Christine , Merlen Emilie , Drouard Magali , Aubert Sébastien , Vidaud Dominique , Espiard Stéphanie , Vantyghem Marie-Christine 
Published: June 2023

Abstract

Context
In patients with neurofibromatosis type 1 (NF1), guidelines suggest screening for pheochromocytoma by metanephrine measurement and abdominal imaging, which may lead to the discovery of gastroenteropancreatic neuroendocrine tumors (GEP-NETs) and their differential diagnosis, gastrointestinal stromal tumors (GISTs). Other endocrine manifestations such as follicular thyroid carcinoma and primary hyperparathyroidism have also been reported in a few cases.

Objective
This study aimed to describe prevalence and clinical presentation of these manifestations through systematic screening in a large cohort of patients.

Methods
In this monocentric retrospective study, 108 patients with NF1 were included and screened for endocrine manifestations and GISTs. Clinical, laboratory, molecular profile, pathology, and morphologic (abdominal computed tomography scan and/or magnetic resonance imaging) and functional imaging were collected.

Results
Twenty-four patients (22.2% of the cohort, 16 female, mean age 42.6 years) presented with pheochromocytomas that were unilateral in 65.5%, benign in 89.7%, and with a ganglioneural component in 20.7%. Three female patients (2.8% of the cohort, aged 42-63 years) presented with well-differentiated GEP-NETs, and 4 (3.7%) with GISTs. One patient had primary hyperparathyroidism, 1 patient had medullary microcarcinoma, and 16 patients had goiter, multinodular in 10 cases. There was no correlation between pheochromocytoma and other NF1 tumoral manifestations, nor correlations between pheochromocytoma and NF1 genotype, despite a familial clustering in one-third of patients.

Conclusion
The pheochromocytoma prevalence in this NF1 cohort was higher (&gt;20%) than previously described, confirming the interest of systematic screening, especially in young women. The prevalence of GEP-NETs and GISTs was about 3%, respectively. No phenotype–genotype correlation was observed.
Trends in Management of Osteoporosis Following Primary Vertebral Compression Fracture
Authors: Malacon Karen , Beach Isidora , Touponse Gavin , Rangwalla Taiyeb , Lee Jennifer , Zygourakis Corinna 
Published: June 2023

Abstract

Purpose
Osteoporosis affects more than 200 million individuals worldwide and predisposes to vertebral compression fractures (VCFs). Given undertreatment of fragility fractures, including VCFs, we investigate current anti-osteoporotic medication prescribing trends.

Methods
Patients 50 and older with a diagnosis of primary closed thoracolumbar VCF between 2004 and 2019 were identified from the Clinformatics® Data Mart database. Multivariate analysis was performed for demographic and clinical treatment and outcome variables.

Results
Of 143 081 patients with primary VCFs, 16 780 (11.7%) were started on anti-osteoporotic medication within a year; 126 301 (88.3%) patients were not started on medication. The medication cohort was older (75.4 ± 9.3 vs 74.0 ± 12.3 years, P &lt; .001), had higher Elixhauser Comorbidity Index scores (4.7 ± 6.2 vs 4.3 ± 6.7, P &lt; .001), was more likely to be female (81.1% vs 64.4%, P &lt; .001), and was more likely to have a formal osteoporosis diagnosis (47.8% vs 32.9%) than the group that did not receive medication. Alendronate (63.4%) and calcitonin (27.8%) were the most commonly initiated medications. The proportion of individuals receiving anti-osteoporotic medication within the year following VCF peaked in 2008 (15.2%), then declined until 2012 with a modest increase afterward.

Conclusions
Osteoporosis remains undertreated after low-energy VCFs. New anti-osteoporotic medication classes have been approved in recent years. Bisphosphonates remain the most prescribed class. Increasing recognition and treatment of osteoporosis is paramount to decreasing the risk of subsequent fractures.
Pengaruh Durasi Penggenangan Selama Fase Vegetatif Akhir Terhadap Pertumbuhan Tanaman Cabai (Capsicum frutescens L ) Varietas Sonar
Authors: Sayekti Sri Wigati Lambang , Setiari Nintya , Darmanti Sri , Saptiningsih Endang 
Published: June 2023

Capsicum frutescens L merupakan tanaman yang banyak dibudidayakan di Indonesia karena memiliki nilai ekonomi tinggi. Salah satu kendala dalam produksi cabai rawit adalah faktor lingkungan antara lain cekaman genangan. Cekaman genangan terjadi akibat adanya curah hujan yang tinggi. Tujuan dari penelitian ini untuk mengetahui pengaruh durasi penggenangan terhadap pertumbuhan cabai rawit varietas Sonar pada fase vegatatif akhir. Penelitian dilakukan dengan empat perlakuan, yaitu kontrol, durasi penggenangan 1, 3, dan 10 hari. Masing-masing perlakuan terdiri dari lima ulangan. Desain penelitian yang digunakan adalah RAL dengan satu faktor yaitu durasi penggenangan. Analisis data setelah penggenangan menggunakan uji T taraf signifikansi 5% dan setelah fase pemulihan selama 30 hari menggunakan uji one-way ANOVA yang diteruskan ke uji LSD taraf signifikansi 5%. Berdasarkan data yang dihasilkan menunjukkan bahwa setelah penggenangan terjadi penghambatan pertumbuhan tanaman meliputi panjang akar, jumlah daun, ukuran daun, tinggi tanaman, dan adanya perubahan warna daun serta penurunan bobot segar tajuk dan bobot segar akar. Penggenangan juga memperpanjang fase vegatatif tanaman sehingga menunda terbentuknya tunas bunga. Pertumbuhan tanaman cabai masih dapat berlangsung setelah cekaman penggenangan durasi 1, 3 dan 10 hari. Setelah fase pemulihan, terjadi peningkatan pertumbuhan tanaman. Capsicum frutescens L was a plant that is widely cultivated in Indonesia because it has high economic value. One of the obstacles in the production of cayenne pepper is environmental factors such as flooding stress. Flooding stress occurs due to high rainfall. The purpose of the study was to determine the effect of flooding duration on the growth of Sonar cayenne pepper in the late vegetative phase. The study was conducted with four treatments, namely control, flooding duration 1, 3, and 10 days. Each treatment consisted of five replications. The research design used was RAL with one factor, namely the duration of flooding. Analysis of the data after flooding used the T-test with a significance level of 5% and after the recovery phase for 30 days used the one-way ANOVA test which was continued to the LSD test with a significance level of 5%. Based on the resulting data, it was shown that after flooding there was inhibition of plant growth including root length, number of leaves, leaf size, plant height, and changes in leaf color as well as a decrease in shoot fresh weight and root fresh weight. Flooding also prolongs the vegetative phase of the plant thereby delaying the formation of flower buds. Chili plant growth can still take place after flooding stress duration of 1, 3, and 10 days. After the recovery phase, there was an increase in plant growth.
Pengaruh Suplementasi Kurkumin dan Injeksi Hormon Tiroksin Pada Ikan Mas (Cyprinus carpio L) Terhadap Konsentrasi Hormon Tiroksin dan Laju Pertumbuhan Spesifik
Authors: Rawung Livana Dethris , Rompas Christny Ferdina Evie 
Published: June 2023

Hormon tiroksin merupakan salah satu jenis hormon tiroid yang berperan penting dalam merangsang pertumbuhan dan perkembangan gonad. Sedangkan kurkumin merupakan senyawa bioaktif yang dapat meningkatkan proses reproduksi pada ikan. Penelitian ini bertujuan untuk mengetahui profil konsentrasi hormon tiroksin dan pertumbuhan spesifik ikan uji yang diberi suplementasi kurkumin dan penyuntikan hormon tiroksin. Ikan mas betina yang digunakan dalam penelitian ini sebanyak 40 ekor. Rancangan percobaan yang digunakan adalah rancangan acak lengkap dengan faktorial 4 x 2. Faktor pertama adalah dosis kurkumin yang terdiri dari 4 konsentrasi (0; 2.5; 5; 10 g curcumin/kg feed), dan faktor kedua adalah hormon tiroksin yang terdiri dari 2 konsentrasi (0 dan 0.1 µg.g-1 bobot badan). Secara statistik data hasil penelitian menunjukkan bahwa suplementasi kurkumin dan penyuntikan hormon tiroksin berpengaruh nyata (p<0,05) terhadap konsentrasi hormon tiroksin dan laju pertumbuhan spesifik. Penelitian ini menunjukkan bahwa injeksi hormon tiroksin dan suplementasi kurkumin dapat meningkatkan konsentrasi hormon tiroksin dalam serum, dan hasil ini juga menunjukkan bahwa status reproduksi ikan uji berpengaruh terhadap peningkatan konsentrasi hormon tiroksin dan laju pertumbuhan spesifik. Thyroxine hormone is one type of thyroid hormone that plays an important role in stimulating growth and gonad development. Otherwise, curcumin is a bioactive compound that can improve the process of reproduction in fish. The aim of this study is to know the profile of the thyroxine hormone concentration and specific growth of tested fish given supplementation of curcumin and injection of thyroxine hormone. 40 female carp were used in this study. The experimental design used was a completely randomized design with a 4 x 2 factorial. The first factor was the dose of curcumin (0; 2.5; 5; 10 g curcumin/kg feed), and the second factor was thyroxine hormone (0 and 0.1 µg.g-1 body weight). Statistically, showed that the supplementation of curcumin and injection of thyroxine hormone had a significant effect (p<0.05) on the concentration of the thyroxine hormone and the specific growth rate. This study showed that the injection of the thyroxine hormone and supplementation of curcumin can increase the concentration of the thyroxine concentration in serum, and these results also showed that the reproduction status of the tested fish also influenced the increase of the thyroxine hormone concentration in serum and the specific growth rate (SGR).
Uji Fertilitas Tikus (Rattus norvegicus) Jantan Setelah Paparan Ekstrak Etanol Daun Mimba (Azadirachta Indica)
Authors: Sitasiwi Agung Janika , Saraswati Tyas Rini , Alauddin Faisal Mahmud 
Published: June 2023

Daun mimba (Azadirachta indica) mengandung senyawa antifertilitas yang dapat menekan kemampuan proses reproduksi baik pada jantan atau betina. Tujuan penelitian ini adalah untuk menguji ekstrak etanol daun mimba (A. indica) terhadap fertilitas tikus jantan dengan mengamati bobot testis, rasio kebuntingan, jumlah anak dan penurunan jumlah anak. Penelitian eksperimental ini dilakukan dengan rancangan acak lengkap menggunakan 32 ekor tikus jantan dan 36 ekor tikus betina. Hewan uji dibagi menjadi 4 kelompok, masing-masing dengan 6 ulangan. Kelompok P0 adalah kontrol dengan hewan uji yang hanya diberi akuades, sedangkan P1, P2 dan P3 merupakan kelompok perlakuan yang masing-masing diberi ektrak etanol daun mimba dengan dosis 60, 80 and 100 mg/kgBB/hari. Bahan uji diberikan secara oral dengan volume 1ml selama 14 hari. Setengah dari jumlah hewan uji digunakan untuk pengamatan parameter bobot testis. Isolasi testis dilakukan setelah pemberian bahan uji berakhir. Uji kawin dilakukan pada hewan uji yang tersisa pada setiap kelompok perlakuan. Hasil penelitian menunjukan bahwa tidak ada perbedaan nyata (p>0,05) pada bobot testis antara kelompok kontrol dan kelompok perlakuan, tetapi terjadi perbedaan nyata (p<0.05) penurunan rasio kebuntingan, jumlah anak dan persentase penurunan jumlah anak yang dihasilkan. Berdasar hasil penelitian dapat disimpulkan bahwa tikus jantan mengalami penurunan fertilitas setelah pemberian ekstrak etanol daun mimba dengan dosis 60, 80 dan 100 mg/kgBB selama 14 hari. Neem leaves (Azadirachta indica) contain antifertility compounds that can suppress the ability of the reproductive process, both on male and female. The purpose of this study is to examine the ethanolic extract of Neem leaves (A. indica) on male rat fertility through observation in testicular weight, pregnancy ratio, litter size and litter size decrease. This experimental study using a completely randomized design (CRD), with 32 male rats and 36 females divided into 4 treatment groups with 6 replication on each. The P0 group is control group which treated with aquadest only; the treatment are P1, P2, and P3 which treated with ethanolic Neem leaves extract 60, 80 and 100 mg/kgBW/day. The treatment are given orally with 1 ml in volume, for 14 consecutive days. A half of total animal in every group were used to get the testis weight parameter. Testicular isolation is done after the treatment. The other animals of were continued with mating test. The result showed that there were no significant differences (P>0.05) in the testicular weight of control group compared to treated groups, but there were significant differences (P<0,05) on pregnancy ratio, litter size and the percentage of litter size decrease. The conclusion of this study is the fertility of male rats were decreased by administration of neem leaves ethanolic extract at doses of 60, 80, and 100 mg/kgBB with 14 consecutive days exposure time.
UJi Kualitatif Metabolit Sekunder pada Beberapa Tanaman yang Berkhasiat sebagai Obat Tradisional
Authors: Khafid Abdul , Wiraputra Muhammad Dwijunianto , Putra Agita Christyaji , Khoirunnisa Nafiah , Putri Aurora Awalia Kirana , Suedy Sri Widodo Agung , Nurchayati Yulita 
Published: June 2023

Indonesia sebagai negara yang memiliki megabiodiversitas terbesar kedua saat ini memberikan manfaat yang begitu luar biasa. Diantara banyak tumbuhan yang ada, tidak sedikit pula tumbuhan yang bisa dimanfaatkan sebagai obat-obatan karena senyawa metabolit sekunder yang terkandung pada berbagai organnya, salah satunya adalah pada bagian daun. Beberapa senyawa metabolit sekunder memiliki khasiat obat, seperti flavonoid, saponin, alkaloid, steroid, triterpenoid dan tanin. Penelitian ini bertujuan untuk mengetahui senyawa metabolit sekunder yang terkandung di dalam daun beberapa tumbuhan berkhasiat obat, antara lain Salam (Syzygium polyanthum (Wight) Walp.), Salam Koja (Murraya koenigii), Sirih (Piper betle Linn), Pepaya (Carica papaya L.), dan Jeruk Purut (Citrus hystrixD. C.). Penelitian dilaksanakan dengan melakukan uji identifikasi senyawa metabolit sekunder pada eksrak maserasi beberapa sampel daun dalam pelarut etanol 70%. Hasil penelitian menunjukkan pada uji flavonoid, saponin, alkaloid, dan tanin positif pada kelima sampel daun, uji triterpenoid positif pada daun Jeruk Purut, uji steroid positif pada semua sampel kecuali daun Jeruk Purut. Indonesia as a country that has the second-largest mega biodiversity currently provides extraordinary benefits. Among the many plants that exist, there are so many plants that can be used as medicines because of secondary metabolites contained in various organs, one of which is in the leaves. Some of the secondary metabolic compounds had medicinal properties, such as flavonoids, saponins, alkaloids, steroids, triterpenoids, and tannins. This study aims to determine the secondary metabolite compounds contained in leaves of several plants with medicinal benefits, such as Bay Leaves (Syzygium polyanthum (Wight) Walp.), Curry Tree (Murraya koenigii), Betel (Piper betle Linn), Papaya (Carica papaya L.) and Kaffir lime (Citrus hystrix DC). The research was done by identification test of secondary metabolites in the macerated extract of several leaf samples in 70% ethanol solvent. The results showed a positive flavonoid, saponin, alkaloid and tanin on all five-leaf samples, positive triterpenoid test on kaffir lime leaves, positive steroid test on all samples except kaffir lime leaves.
Potensi Mangrove Avicennia Marina (Forsk.) sebagai Agen Fitoremidiasi Kadmium (Cd) di Tambak dan Laut Mangunharjo, Kecamatan Tugu, Kota Semarang
Authors: Hastuti Endah Dwi , Afiyah Fina Irodatul , Izzati Munifatul 
Published: June 2023

Aktivitas industri di perairan Mangunharjo menghasilkan limbah yang dapat menyebabkan pencemaran logam berat salah satunya yaitu kadmium (Cd). Mangrove Avicennia marina dapat mengabsorbsi Cd dari sedimen dan air melalui akar kemudian di translokasi ke organ lain seperti daun dan akar sehingga berpotensi sebagai fitoremidiasi. Penelitian ini bertujuan untuk menganalisis kemampuan akumulasi Cd pada akar dan daun mangrove Avicennia marina di perairan laut dan tambak Mangunharjo. Penelitian ini menggunakan RAL dengan 2 faktor yaitu lokasi (tambak dan laut) dan organ (daun dan akar) masing-masing perlakuan dengan 3 ulangan. Analisis kandungan Cd menggunakan metode AAS (Atomic Absorption Spechtophotometry), data diuji dengan ANOVA, dilanjutkan dengan DMRT pada taraf uji 5 %. Hasil penelitian menunjukkan bahwa di tambak kadar Cd pada sedimen yaitu 1.0897 ppm dan pada air 0.0123 ppm, sedangkan di laut kadar Cd pada sedimen yaitu 0.1193 ppm dan pada air 0.0163 ppm. Faktor organ mangrove dan lokasi menujukkan interaksi terhadap kadar Cd dan nilai BCF. Kadar Cd tertinggi terdapat pada organ daun di tambak (0.2269 ppm) dan nilai terendah terdapat pada organ daun di laut (0.0200 ppm). Nilai BCF tertinggi terdapat pada organ akar di laut (0.5133) dan yang paling rendah adalah organ daun di laut (0.0567). Industrial activities in the waters of Mangunharjo produce waste that can cause heavy metal pollution, one of which is cadmium (Cd). Avicennia marina mangroves can absorb Cd from sediment and water through the roots and then translocated to other organs such as leaves and roots so that it has the potential as phytoremediation. This study aimed to analyze the ability of Cd accumulation in the roots and leaves of Avicennia marina mangroves in Ponds and Mangunharjo Seas. This study used RAL with 2 factors, namely location (ponds and sea) and organs (leaves and roots) each treatment with 3 replications. Analysis of Cd content using AAS (Atomic Absorption Spechtophotometry) method, data was tested by ANOVA, followed by DMRT at the 5% test level. The results showed that in ponds the Cd content in sediment was 1.0897 ppm and in water was 0.0123 ppm, while at sea the Cd level in sediment was 0.1193 ppm and in water was 0.0163 ppm. Mangrove organ factors and location showed an interaction with Cd levels and BCF values. The highest Cd levels were found in leaf organs in ponds (0.2269 ppm) and the lowest values were found in leaf organs in the sea (0.0200 ppm). The highest BCF value was found in root organs in the sea (0.5133) and the lowest was in leaf organs in the sea (0.0567).