bluuebunny/crossref_metadata_2025_split huggingface dataset:
Antithrombotics prescription pattern and clinical outcomes in atrial fibrillation patients with type 2 diabetes mellitus
Authors: Hoo Y Y , Mazlan-Kepli W , Habizal N H , Ismail M I , Wong Y P , Rosnan N H , Mohamed S , Abd Ghani A R , Mohd Yusof H , Abdullah Ramaiah A R , Selvaraj K , Abd Malek A M , Abdul Ghapar A K 
Published: January 2023

Abstract

Funding Acknowledgements
Type of funding sources: None.

Background
Atrial fibrillation (AF) and Diabetes mellitus (DM) often coexist. As DM is considered while calculating the thromboembolic risk for AF patients, little is known about the prescription pattern of antithrombotic therapies in patients with AF and DM and their clinical outcomes.

Purpose
In this subsidiary study, we examined the prescription patterns of antithrombotic therapies and clinical outcomes of AF patients with type 2 DM.

Methods
We analyzed data from a single-center cohort of patients with a primary diagnosis of AF in a tertiary cardiac referral hospital in Malaysia from 1st January 2018 to 31st December 2020. Patients' clinical data and information related to antithrombotic therapy were traced through electronic Hospital Information system. A data collection form was used for data collection. The primary endpoint of the study was a composite cardiovascular (CV) event which consists of all-cause mortality, acute coronary syndrome (ACS), ischemic stroke and transient ischemic attack (TIA). The safety endpoint of the study was a bleeding event, defined as hemoglobin drop more than 2 g/dl, blood transfusion and bleeding at critical area.

Results
Of the 1006 AF patients (59.2% male; mean age 64.2 (12.1) years), 400 (39.8%) had a history of DM. Of these, 45.8% (n=183) were using warfarin; 46.5% (n=159) used direct oral anticoagulant (DOAC), 54.0% (n=216) used a single antiplatelet, 11.5% (n=46) used a double antiplatelet, 8.0% (n=32) used triple therapy which consists of two antiplatelet agents with one anticoagulant. The use of single antiplatelet agent (54.0% vs 46.4%, p=0.018) and double antiplatelet agents (11.5% vs 7.1%, p=0.016) was significantly associated with AF with DM patients, whereas there was no association between anticoagulant use and AF patients with or without DM (85.5% vs 82.5%, p=0.209). There was no association in composite CV events between AF patients with or without DM (12.0% vs 10.4%, p=0.427). The proportion of subjects who reported having bleeding events were also did not differ by the presence of AF patients with or without DM. (4.5% vs 2.8%, p=0.151).

Conclusion
Diabetes was associated with increased use of antiplatelet agents; however, DM was not associated with increased risk of composite CV events and bleeding events in patients with concurrent AF. The lack of an association between diabetes and CV risk contrasts with previous research, which could be due to improved diabetes treatment in this cohort of patients with relatively low fasting sugar readings. Further study on the degree of blood glucose as measured by glycosylated hemoglobin (HbA1c) is needed to confirm the finding.
Comparison of risk of hyperkalemia between SGLT2 inhibitors and DPP4-inhibitors in patients with type 2 diabetes
Authors: Wu M Z , Ren Q W , Huang J Y , Tse Y K , Yu S Y , Cheang L F , Li H L , Chan Y H , Tse H F , Yiu K H 
Published: January 2023

Abstract

Funding Acknowledgements
Type of funding sources: None.

Background
Hyperkalemia is a common complication and increases the risk of cardiac arrhythmias and mortality in patients with type 2 diabetes (T2DM), especially in those with diabetic nephropathy. We investigated the risk of hyperkalemia in patients initiated on SGLT2 inhibitors versus DPP-4 inhibitors among patients with T2DM.

Methods
This study included patients with T2DM who initiated on SGLT2 inhibitors or DPP-4 inhibitors between January 01, 2015 and December 31, 2019 from a territory-wide clinical registry in Hong Kong (Clinical Data Analysis and Reporting System [CDARS]). A multivariable cox proportional hazards analysis, adjusting for key confounders, was used to compare the risk of central laboratory-determined hyperkalemia (serum potassium ≥6.0mmol/L) and hypokalemia (serum potassium &lt;3.5mmol/L), respectively, between SGLT2 inhibitors and DPP-4 inhibitors.

Results
10193 new users of SGLT2 inhibitors were matched to 17305 new users of DPP-4 inhibitors. During the 2-year follow-up, there were 104 hyperkalemia events (incident rate [IR] = 5.17 per 1000 person-years) among SGLT2 inhibitors and 306 events (IR = 9.09 per 1000 person-years) among DPP-4 inhibitors, of which SGLT2 inhibitors were associated with a lower risk of incident hyperkalemia (Adjusted HR: 0.66 [95%CI 0.53-0.83], p&lt;0.001), compared to DPP-4 inhibitors. The incident hypokalemia was similar between SGLT2 inhibitors and DPP-4 inhibitors (Adjusted HR: 0.91 [95%CI 0.81-1.03], P=0.13).

Conclusion
SGLT2 inhibitors reduced incident hyperkalemia, but without increasing incident hypokalemia compared to DPP-4 inhibitors.
Differential expression of TLR4 in patients of coronary artery disease with and without traditional cardiovascular risk factors
Authors: Biswas N , Roy D , Karmakar S , Bhowmik R , Mazumdar H , Banerjee A , Roy A , Dey S 
Published: January 2023

Abstract

Funding Acknowledgements
Type of funding sources: None.

Background
Atherosclerosis is inherently an inflammatory process, with a complex interplay of inflammatory markers. It is established that these inflammatory markers play an important role in patients of Coronary Artery Disease(CAD) with traditional cardiovascular risk factors. However, the role of inflammation in the atherosclerotic process in patients of CAD without traditional risk factors is still not clearly known.

Purpose
Our purpose was to determine whether in patients of CAD without traditional risk factors, TLR4(Toll like receptor 4) expression as a marker of inflammation is similar to that in patients of CAD with traditional risk factors.

Materials and Methods
This observational cross sectional study was done between July’20 to Dec’21. Equal number of patients of CAD with and without traditional cardiovascular risk factors undergoing Coronary Artery Bypass Grafting (CABG) were enrolled. The risk factors considered were Hypertension, Diabetes, Dyslipidemia, Obesity and Addiction to Tobacco and/or Alcohol. A minimum of two punch biopsy samples of aortic tissue was taken from each subject undergoing CABG. Immunohistochemistry for TLR4, was done in Ventana BenchMark GX System. The primary TLR4 antibody was procured from reputed source.

Results
The presence or absence of TLR4 expression was associated significantly with the Syntax scores (37.40±4.74 vs 29.5±8.71; p value=0.036), total Cholesterol (187±35.06mg/dL vs 130±35.69 mg/dL ;p value 0.010) and LDL cholesterol (118.86±28.12mg/dL vs 64.21±25.61 mg/dL; p value 0.003). TLR4 expression, however, was not significantly associated with the number of coronary vessels involved (p=0.298). TLR4 expression was also not significantly associated with any other individual risk factors. However, when the traditional risk factors were considered in combination, TLR4 expression was associated significantly with the number of risk factors present(p=0.029) the strongest being in those having 4 traditional risk factors. The level of TLR4 expression gradually declined with the decrease in the number of risk factors, having mostly weak or negative expression in patients without any traditional risk factors.

Conclusion
Patients with CAD without any traditional risk factors, had a less severe coronary artery disease as manifested by lower Syntax scores, and had lower degree of TLR4 expression. Patients with CAD with traditional risk factors had more severe coronary artery disease as evidenced by higher Syntax scores, and had higher degree of TLR4 expression proportional to the number of traditional risk factors present. Thus, differential TLR4 expression in CAD patients with and without traditional risk factors indicated a difference in inflammatory state between the two groups and warrants further investigation.
Long-term effects of e-Health secondary prevention on cardiovascular health: a systematic review and meta-analysis
Authors: Su Jing Jing , Liu Justina Yat Wa , Cheung Daphne Sze Ki , Wang Shanshan , Christensen Martin , Kor Patrick Pui Kin , Tyrovolas Stefanos , Leung Angela Yee Man 
Published: January 2023

Abstract

Aims
Despite the well-documented short-to-medium-term effectiveness of e-Health (electronic health) secondary prevention interventions on patients with cardiovascular disease (CVD), there is limited empirical evidence regarding long-term effectiveness. This review aims to evaluate the long-term effects of e-Health secondary prevention interventions on the health outcomes of patients with CVD.

Methods and results
This systematic review and meta-analysis followed Cochrane Handbook for Systematic Reviews of Interventions. EMBASE, Medline, Web of Science, and Scopus were searched from 1990 to May 2022. Randomized controlled trials investigating the effects of e-Health secondary prevention on health outcomes of CVD patients that collected endpoint data at ≥ 12 months were included. RevMan 5.3 was used for risk of bias assessment and meta-analysis. Ten trials with 1559 participants were included. Data pooling suggested that e-Health programmes have significantly reduced LDL cholesterol [n = 6; SMD = −0.26, 95% confidence interval (CI): (−0.38, −0.14), I2 = 17%, P &lt; 0.001]; systolic blood pressure [n = 5; SMD = −0.46, 95% CI: (−0.84, −0.08), I2 = 90%, P = 0.02]; and re-hospitalization, reoccurrence, and mortality [risk ratio = 0.36, 95% CI: (0.17, 0.77), I2 = 0%, P = 0.009]. Effects on behavioural modification, physiological outcomes of body weight and blood glucose, and quality of life were inconclusive.

Conclusion
e-Health secondary prevention is effective in improving long-term management of risk factors and reducing the reoccurrence of cardiac events in patients with CVD. Results are inconclusive for behaviour modification and quality of life. Exploring, implementing, and strengthening strategies in e-Health secondary prevention programmes that focus on maintaining behaviour changes and enhancing psychosocial elements should be undertaken.

Registration
PROSPERO CRD42022300551.
Detection efficiency of an autonomous underwater glider carrying an integrated acoustic receiver for acoustically tagged Pacific herring
Authors: Cypher Alysha D , Statscewich Hank , Campbell Robert , Danielson Seth L , Eiler John , Bishop Mary Anne 
Published: January 2023

AbstractAutonomous underwater vehicles (AUVs) or gliders are increasingly being used with acoustic telemetry to elucidate fish movements while collecting simultaneous environmental data. We assessed the utility of an AUV equipped with an integrated acoustic receiver to detect Pacific herring (Clupea pallasii) in Prince William Sound, AK, USA. A range test evaluated the effect of glider flight characteristics and environmental conditions on the detection efficiency of transmitters at varying depths. While distance from transmitters was the strongest predictor of detections, glider depth had a variable effect on detection efficiency which depended on transmitter depth and dive orientation. The detection efficiency of the glider-mounted acoustic receiver was less affected by wind speed and water level than that of stationary acoustic receivers deployed within the study area. The AUV also performed repeated, adaptive transects in an area of ∼630 km2 area and detected 30 Pacific herring transmitters without a priori knowledge of their locations. Of these herring transmitters, 14 were presumed shed after repeated detections within the same area, and 2 were detected at multiple locations. This study is the first to demonstrate that glider-mounted acoustic receivers have high detection efficiency for transmitters at varying depths and can detect movements of migratory forage fish in large search areas.
Preschool Pain Management Program for Young Children with Sickle Cell Disease: A Pre–Post Feasibility Study
Authors: Johnston Julia D , Schatz Jeffrey , Bills Sarah E , Frye Bridgett G , Carrara Gabriela C 
Published: January 2023

Abstract

Objective
Vaso-occlusive pain crises in sickle cell disease (SCD) often begin in early childhood. We developed an online pain management intervention to teach caregivers of preschool-aged children with SCD behavioral pain management strategies. The feasibility study goals were to examine response to recruitment, barriers to participation, engagement, acceptability and perceived usefulness of the intervention, and suitability of outcome measures.

Methods
Caregivers of children aged 2.0–5.9 years with access to text messaging and a device to access online videos were recruited from a Southeastern outpatient hematology clinic for a 12-week intervention consisting of pain management videos. Videos taught caregivers behavioral pain management strategies and adaptive responses to pain. Workbook activities helped tailor strategies to their child. Caregivers completed process measures as well as baseline and follow-up measures of pain catastrophizing (Pain Catastrophizing Scale—Parent Report) and responses to their child’s pain (Adult Response to Children’s Symptoms).

Results
Fifty percent (10 of 20) of eligible parents enrolled. Caregivers partially completed (N = 6), completed (N = 3), or did not engage (N = 1) in the intervention. Caregivers who engaged in the program reported implementing the pain management strategies. The intervention was rated as high quality, relevant, and useful. Measures of pain catastrophizing and responses to their child’s pain appeared sensitive to change.

Conclusions
The intervention to promote adaptive coping to pain was acceptable and feasible for caregivers though we found barriers to delivering the intervention to parents. Evaluation of a modified version of the program is indicated to assess implementation issues and effectiveness.
Non-Rabl chromosome organization in endoreduplicated nuclei of barley embryo and endosperm tissues
Authors: Nowicka Anna , Ferková Ľuboslava , Said Mahmoud , Kovacik Martin , Zwyrtková Jana , Baroux Célia , Pecinka Ales 
Published: January 2023

Abstract
Rabl organization is a type of interphase chromosome arrangement with centromeres and telomeres clustering at opposite nuclear poles. Here, we analyzed nuclear morphology and chromosome organization in cycling and endoreduplicated nuclei isolated from embryo and endosperm tissues of developing barley seeds. We show that endoreduplicated nuclei have an irregular shape, less sister chromatid cohesion at 5S rDNA loci, and a reduced amount of centromeric histone CENH3. While the chromosomes of the embryo and endosperm nuclei are initially organized in Rabl configuration, the centromeres and telomeres are intermingled within the nuclear space in the endoreduplicated nuclei with an increasing endoreduplication level. Such a loss of chromosome organization suggests that Rabl configuration is introduced and further reinforced by mitotic divisions in barley cell nuclei in a tissue- and seed age-dependent manner.
Anu Taila, an herbal nasal-drop, delays spore germination in Cunninghamella bertholletiae by reducing cAMP-PKA dependent ROS in mucorale pathogen and extrinsic ROS in human host cells
Authors: Balkrishna Acharya , Sengupta Sohan , Kumari Priya , Dev Rishabh , Haldar Swati , Varshney Anurag 
Published: January 2023

Abstract
The rare, fastest-germinating, frequently invasive mucorale, Cunninghamella bertholletiae, is intractable due to its imprecise etiology. Cunninghamella bertholletiae spores can infect both immunocompromised and immunocompetent individuals to cause mucormycosis. Sub-optimal drug-susceptibility further limits its treatment options. The classical nasal drop, Anu Taila, is reported to be effective against the rather prevalent mucorales, Mucor spp., making its anti-mucormycotic effect against C. bertholletiae worth testing. The inhibitory effect of Anu Taila against C. bertholletiae was manifested as microstructural alterations of the spores and their delayed germination. Anu Taila reduced the germination-promoting reactive oxygen species (ROS) levels in both the pathogen, C. bertholletiae, and the human host lung epithelial A549 cells. Expressions of structural (chitin synthase, trehalose synthase) and functional (cAMP-PKA) markers of spore germination were regulated by Anu Taila. cAMP-PKA expression and ROS generation are well-correlated, implicating the role of Anu Taila in delaying C. bertholletiae spore germination by targeting cAMP-PKA-mediated ROS generation. In conclusion, this study demonstrates that Anu Taila can create an opportunity for the host immune system to tackle the onset of C. bertholletiae infection by delaying its pathogenesis. This can be further leveraged to reinforce the host immune system through combinatorial treatment to prevent the establishment of the mucormycosis infection.
Detection of the Crab Nebula using a randomforest analysis of the first TAIGA IACT data
Authors: Blank M , Tluczykont M , Porelli A , Mirzoyan R , Wischnewski R , Awad A K , Brückner M 
Published: January 2023

ABSTRACT
The Tunka Advanced Instrument for Gamma- and cosmic-ray Astronomy (TAIGA) is a multicomponent experiment for the measurement of TeV to PeV gamma- and cosmic rays. Our goal is to establish a novel hybrid direct air shower technique, sufficient to access the energy domain of the long-sought Pevatrons. The hybrid air Cherenkov light detection technique combines the strengths of the HiSCORE shower front sampling array, and two ∼4 m class, ∼9.6° field of view Imaging Air Cherenkov Telescopes (IACTs). The HiSCORE array provides good angular and shower core position resolution, while the IACTs provide the image shape and orientation for gamma-hadron separation. In future, an additional muon detector will be used for hadron tagging at ≥100 TeV energies. Here, only data from the first IACT of the TAIGA experiment are used. A randomforest algorithm was trained using Monte Carlo (MC) simulations and real data, and applied to $85\, \mathrm{h}$ of selected observational data tracking the Crab Nebula at a mean zenith angle of 33.5°, resulting in a threshold energy of 6 TeV for this data set. The analysis was performed using the gammapy package. A total of 163.5 excess events were detected, with a statistical significance of 8.5 σ. The observed spectrum of the Crab Nebula is best fit with a power law above 6 TeV with a flux normalization of (3.20 ± 0.42) · 10−10 TeV−1 cm−2 s−1at a reference energy of $13\, \mathrm{TeV}$ and a spectral index of −2.74 ± 0.16.
Thermal and reionization history within a large-volume semi-analytic galaxy formation simulation
Authors: Balu Sreedhar , Greig Bradley , Qiu Yisheng , Power Chris , Qin Yuxiang , Mutch Simon , Wyithe J Stuart B 
Published: January 2023

ABSTRACTWe predict the 21-cm global signal and power spectra during the Epoch of Reionization using the meraxes semi-analytic galaxy formation and reionization model, updated to include X-ray heating and thermal evolution of the intergalactic medium. Studying the formation and evolution of galaxies together with the reionization of cosmic hydrogen using semi-analytic models (such as M eraxes) requires N-body simulations within large volumes and high-mass resolutions. For this, we use a simulation of side-length 210 h−1 Mpc with 43203 particles resolving dark matter haloes to masses of $5\times 10^8 \rm{ }h^{-1}\, \mathrm{M_\odot }$. To reach the mass resolution of atomically cooled galaxies, thought to be the dominant population contributing to reionization, at z = 20 of $\sim 2\times 10^7 \text{ }h^{-1}\, \mathrm{M_\odot }$, we augment this simulation using the darkforest Monte Carlo merger tree algorithm (achieving an effective particle count of ∼1012). Using this augmented simulation, we explore the impact of mass resolution on the predicted reionization history as well as the impact of X-ray heating on the 21-cm global signal and the 21-cm power spectra. We also explore the cosmic variance of 21-cm statistics within 703 h−3 Mpc3 sub-volumes. We find that the midpoint of reionization varies by Δz ∼ 0.8 and that the cosmic variance on the power spectrum is underestimated by a factor of 2–4 at k ∼ 0.1–0.4 Mpc−1 due to the non-Gaussian nature of the 21-cm signal. To our knowledge, this work represents the first model of both reionization and galaxy formation which resolves low-mass atomically cooled galaxies while simultaneously sampling sufficiently large scales necessary for exploring the effects of X-rays in the early Universe.
Hepatic and pulmonary involvement in a patient with PR3-ANCA vasculitis following SARS-CoV-2 vaccination: A case report
Authors: Tonutti Antonio , Simonetta Edoardo , Stainer Anna , Suigo Giulia , De Santis Maria , Selmi Carlo , Masetti Chiara , Lleo Ana , Terracciano Luigi Maria , Aliberti Stefano , Amati Francesco 
Published: January 2023

ABSTRACTWe here report the first case of anti-proteinase 3–positive anti-neutrophil cytoplasmic antibodies (ANCA)-associated vasculitis following the severe acute respiratory syndrome coronavirus 2 Pfizer-BioNTech vaccine presenting with prominent liver involvement and alveolar haemorrhage. Two weeks after vaccination, a 49-year-old man developed inflammatory arthralgias and hypertransaminasaemia. Two months later, fever and haemoptysis appeared; the patient tested positive for anti-proteinase 3 autoantibodies. High-dose steroids and rituximab were started, and complete remission was achieved. Systemic autoimmune diseases, including ANCA-associated vasculitis, should always be considered in the differential diagnosis of hypertransaminasaemia, especially when the clinical context is suspicious.
Mitochondrial ferredoxin-like is essential for forming complex I-containing supercomplexes in Arabidopsis
Authors: Röhricht Helene , Przybyla-Toscano Jonathan , Forner Joachim , Boussardon Clément , Keech Olivier , Rouhier Nicolas , Meyer Etienne H 
Published: January 2023

AbstractIn eukaryotes, mitochondrial ATP is mainly produced by the oxidative phosphorylation (OXPHOS) system, which is composed of 5 multiprotein complexes (complexes I–V). Analyses of the OXPHOS system by native gel electrophoresis have revealed an organization of OXPHOS complexes into supercomplexes, but their roles and assembly pathways remain unclear. In this study, we characterized an atypical mitochondrial ferredoxin (mitochondrial ferredoxin-like, mFDX-like). This protein was previously found to be part of the bridge domain linking the matrix and membrane arms of the complex I. Phylogenetic analysis suggested that the Arabidopsis (Arabidopsis thaliana) mFDX-like evolved from classical mitochondrial ferredoxins (mFDXs) but lost one of the cysteines required for the coordination of the iron-sulfur (Fe-S) cluster, supposedly essential for the electron transfer function of FDXs. Accordingly, our biochemical study showed that AtmFDX-like does not bind an Fe-S cluster and is therefore unlikely to be involved in electron transfer reactions. To study the function of mFDX-like, we created deletion lines in Arabidopsis using a CRISPR/Cas9-based strategy. These lines did not show any abnormal phenotype under standard growth conditions. However, the characterization of the OXPHOS system demonstrated that mFDX-like is important for the assembly of complex I and essential for the formation of complex I-containing supercomplexes. We propose that mFDX-like and the bridge domain are required for the correct conformation of the membrane arm of complex I that is essential for the association of complex I with complex III2 to form supercomplexes.