bluuebunny/crossref_metadata_2025_split huggingface dataset:
Power grid investment forecasting method based onPSO‐GRUneural network
Authors: Lou Qihe , Li Yanbin , Liu Ning 
Published: June 2023

SummaryTraditional power grid investment forecasting models often ignore the cyclical characteristics of historical investment data, leading to one‐sided investment allocation results and insufficient model generalization ability. This article proposed a power network investment forecasting method based on particle swarm optimization‐gate recurrent unit (PSO‐GRU) neural network. First, the temporal attention mechanism is introduced into the traditional GRU network, which improves the ability of the network to extract temporal features. Then, in order to avoid the adverse effects of unreasonable parameter configuration on model training, an optimized particle swarm optimization algorithm was proposed to optimize the parameter set of GRU and improve the training accuracy of the model. The data provided by an electric power company in China is used for experimental analysis, and the predicted results are compared with other electric power investment models. The RMSE of the PSO‐GRU model proposed in this article is 0.1223, which is superior to other algorithms and has certain effectiveness.
Conjugation of Fluorochromes to Monoclonal Antibodies
Authors: Lantz Larry , Holmes Kevin , Douagi Iyadh 
Published: June 2023

AbstractDetection of cell surface molecules labeled by monoclonal or polyclonal antibodies conjugated to a fluorochrome is the most widely used application of flow cytometry. Here, we present protocols for tagging monoclonal antibodies with fluorescein, biotin, Texas Red, and phycobiliproteins. In addition, we provide a procedure for preparing a PE‐Texas Red tandem conjugate dye that can then be used for antibody conjugation. These protocols enable investigators to label antibodies of their choice with multiple fluorochromes and permit more combinations of antibodies for multicolor flow applications. © 2023 Wiley Periodicals LLC. This article has been contributed to by U.S. Government employees and their work is in the public domain in the USA.Basic Protocol 1: Labeling an antibody with fluorescein isothiocyanate (FITC)Basic Protocol 2: Labeling an antibody with long‐armed biotinBasic Protocol 3: Labeling an antibody with Texas Red‐XBasic Protocol 4: Labeling an antibody with a synthetic organic fluor kitBasic Protocol 5: Labeling an antibody with phycobiliproteinsBasic Protocol 6: Conjugation of Texas Red to R‐phycoerythrin to produce an energy transfer fluorochrome
Brittleness mechanism of pork meatballs revealed from molecular structure to macroscopic performance
Authors: Li Jiayi , Li Shuchang , Xiao Nan , Tian Xingguo , Guo Shanguang , Cheng Weiwei , Jiang Aimin , Ai Minmin 
Published: June 2023

AbstractMeatballs are manufactured with great craftsmanship, but the brittle quality of meatballs is not resistant to frozen storage, which limits the large‐scale promotion of meatballs for transportation. Meanwhile, the ‘brittle mechanism’ of meatballs is still unclear and it is difficult to solve the scientific problem of brittle quality loss of meatballs from the root. This study revealed the mechanism underlying the brittleness of pork meatballs gel. In this study, the rheological properties, textural quality, molecular forces and microstructure of meatballs were characterized using fresh meat (FsM group), ambient meat group, frozen meat group, and ground meat group. The results showed that the FsM group presented better sensorial characteristics. The rheological properties of the FsM group exhibited higher storage modulus (G′) and loss modulus (G″), indicating better viscoelastic properties. The results of textural determination reflected that the FsM group showed improved brittleness quality and the brittleness was supported by hydrophobic interactions and disulfide bonds. The FsM group with good brittleness quality formed a ‘large cavity’ microstructure. The correlations analysis revealed a correlation between the texture properties of meatballs and their protein molecular forces. Our findings clearly provide new insights into the mechanism underlying the brittleness of meatballs.
Cortical and Trabecular Bone Deficit in Middle‐Aged Men Living withHIV
Authors: Hong Namki , Kim Jung Ho , Treece Graham , Kim Hyeon Chang , Choi Jun Yong , Rhee Yumie 
Published: June 2023

ABSTRACTA significant increase in the risk of hip fracture was observed in middle‐aged men living with human immunodeficiency virus (MLWH), almost a decade earlier than those without infection. Data regarding cortical and trabecular bone deficit of hip, an important determinant of bone strength, in MLWH are limited. Quantitative CT was performed in consecutive MLWH aged ≥30 years between November 2017 and October 2018 at Severance Hospital, Seoul, Korea. Volumetric bone mineral density (vBMD) and cortical bone mapping parameters of hip (cortical thickness [CTh], cortical bone vBMD [CBMD], cortical mass surface density [CMSD], endocortical trabecular density [ECTD]) were compared to age‐matched and body mass index (BMI)‐matched controls (1:2) using a community‐based healthy adults cohort. Among 83 MLWH and 166 controls (mean age: 47.2 years; BMI: 23.6 kg/m2), MLWH had lower total hip vBMD (280 ± 41 versus 296 ± 41 mg/cm3), CMSD (155 versus 160 mg/cm2), and ECTD (158 versus 175 mg/cm3) than controls that remained robust after adjustment for covariates (adjusted β: total hip vBMD, −18.8; CMSD, −7.3; ECTD, −18.0;p < 0.05 for all). Cortical bone mapping revealed localized deficit of CTh, CBMD, and CMSD in the anterolateral trochanteric region and femoral neck in MLWH compared to controls, with a more extensive ECTD deficit. In MLWH, lower CD4 T‐cell count (/100 cells/mm3decrement) and protease inhibitor (PI)‐based regimen (versus non‐PI regimen) at the time of antiretroviral treatment initiation were associated with lower total hip vBMD (adjusted β −7.5 for lower CD4 count; −28.3 for PI‐based regimen) and CMSD (adjusted β −2.6 for lower CD4 count; −12.7 for PI‐based regimen;p < 0.05 for all) after adjustment for covariates including age, BMI, smoking, alcohol use, hepatitis C virus co‐infection, tenofovir exposure, and CT scanner types. MLWH had lower hip bone density with cortical and trabecular bone deficit compared to community‐dwelling controls. © 2023 American Society for Bone and Mineral Research (ASBMR).
Extracellular vesicle‐packaged ILK from mesothelial cells promotes fibroblast activation in peritoneal fibrosis
Authors: Huang Qiang , Sun Yuxiang , Peng Long , Sun Juan , Sha Zixin , Lin Hongchun , Li Yongjie , Li Canming , Li Huiqun , Shang Hongli , Chen Yanxu , Dou Xianrui , Hu Zhaoyong , Ye Zengchun , Peng Hui 
Published: June 2023

AbstractProgressive peritoneal fibrosis and the loss of peritoneal function often emerged in patients undergoing long‐term peritoneal dialysis (PD), resulting in PD therapy failure. Varieties of cell‐cell communications among peritoneal cells play a significant role in peritoneal fibrogenesis. Extracellular vesicles (EVs) have been confirmed to involve in intercellular communication by transmitting proteins, nucleic acids or lipids. However, their roles and functional mechanisms in peritoneal fibrosis remain to be determined. Using integrative analysis of EV proteomics and single‐cell RNA sequencing, we characterized the EVs isolated from PD patient's effluent and revealed that mesothelial cells are the main source of EVs in PD effluent. We demonstrated that transforming growth factor‐β1 (TGF‐β1) can substitute for PD fluid to stimulate mesothelial cells releasing EVs, which in turn promoted fibroblast activation and peritoneal fibrogenesis. Blockade of EVs secretion by GW4869 or Rab27a knockdown markedly suppressed PD‐induced fibroblast activation and peritoneal fibrosis. Mechanistically, injured mesothelial cells produced EVs containing high level of integrin‐linked kinase (ILK), which was delivered to fibroblast and activated them via p38 MAPK signalling pathway. Clinically, the expression of ILK was up‐regulated in fibrotic peritoneum of patients undergoing long‐term PD. The percentage of ILK positive EVs in PD effluent correlated with peritoneal dysfunction and the degree of peritoneal damage. Our study highlights that peritoneal EVs mediate communications between mesothelial cells and fibroblasts to initiate peritoneal fibrogenesis. Targeting EVs or ILK could provide a novel therapeutic strategy to combat peritoneal fibrosis.
FN1 and cancer‐associated fibroblasts markers influence immune microenvironment in clear cell renal cell carcinoma
Authors: Ashok Gayathri , Ramaiah Sudha 
Published: June 2023

AbstractBackgroundAltered tumor microenvironment (TME) is characterized in clear cell renal cell carcinoma (ccRCC) as a result of the heterogeneity observed in the TME. Modulations in TME have shown tumor metastasis promotion; hence, identifying TME‐based biomarkers can be critical for theranostics application.MethodsHere, we performed an integrated systems biology approach utilizing differential gene expression, network metrics and clinical samples cohorts to prioritize the major deregulated genes and their associated pathways specific for metastasis.ResultsThe gene expression profiling of 140 ccRCC samples resulted in 3657 differentially expressed genes, from which a network of 1867 up‐regulated genes were further computed using network metrics for screening hub‐genes. The specific pathways of ccRCC entailed through functional enrichment analysis of the hub‐gene clusters indicated the role of the identified hub‐genes in the enriched pathways, further validating the functional significance of the hub‐genes. The positive correlation of TME cells, namely cancer‐associated fibroblasts (CAFs) and its biomarkers (FAP and S100A4) with FN1, signified the role of hub‐gene signaling for promoting metastasis in ccRCC. Thereafter, comparative expression, differential methylation, genetic alteration and overall survival analysis were analyzed to validate the screened hub‐genes.ConclusionsThe hub‐genes were validated and prioritized by correlating with expression‐based parameters, including histological grades, tumor, metastatic and pathological stages (based on median transcript per million; analysis of variance [ANOVA], P ≤ 0.05) from a clinically curated ccRCC dataset to further substantiate the translational benefits of the screened hub‐genes as potential diagnostic biomarkers for ccRCC.
Efficacy of Dual‐Task Training in Patients with Parkinson's Disease: A Systematic Review with Meta‐Analysis
Authors: García‐López Héctor , de los Ángeles Castillo‐Pintor María , Castro‐Sánchez Adelaida María , Lara‐Palomo Inmaculada Carmen , Obrero‐Gaitán Esteban , Cortés‐Pérez Irene 
Published: June 2023

ABSTRACTBackgroundDual‐task training (DTT) involves simultaneously motor and cognitive exercises.ObjectivesTo determine the effectiveness of DTT, in comparison to other interventions [single‐task training (STT) and usual care (UC)], on gait and balance parameters, motor impairments, activities of daily living (ADLs) and quality of life (QoL) in patients with Parkinson's disease (PD) immediately post‐intervention and at 3, 6, and 12 months after therapy.MethodsA meta‐analysis was performed following PRISMA Guidelines through searching in PubMed, SCOPUS, WOS, CINAHL, SciELO and PEDro up to September 2022. We included randomized controlled trials (RCTs) that compare the effect of DTT versus STT and UC on gait (speed, step and stride length, cadence and steps per day), balance (functional and dynamic balance), motor impairments, ADLs and QoL. Methodological quality was assessed using the PEDro scale. The pooled effect was calculated through Cohen's Standardized Mean Difference (SMD) and its 95% confidence interval (95%CI).ResultsSeventeen RCTs with 826 participants and a mean PEDro score of 6.59 ± 1 points were included. In comparison to STT and UC, DTT is effective in improving walking speed (SMD 0.42, 95%CI 0.23–0.6), stride length (SMD 0.69, 95%CI 0.23–1.15), cadence (SMD 0.41, 95%CI 0.19–0.63), functional balance (SMD 1.15, 95%CI 0.92–1.4), dynamic balance (SMD −0.5, 95%CI −0.81 to −0.18) and motor impairments (SMD −0.86, 95%CI −1.25 to −0.47). No adverse effects related to DTT were reported.ConclusionsDTT is an effective and safe therapy for improving gait, balance and motor impairments in patients with PD.
Gender differences in prosthesis‐related outcomes among veterans: Results of a national survey of U.S. veterans
Authors: Kuo Patty B. , Lehavot Keren , Thomas Rachel M. , Dashtestani Krista , Peterson Alexander C. , Korpak Anna M. , Turner Aaron P. , Williams Rhonda M. , Czerniecki Joseph M. , Norvell Daniel C. , Littman Alyson J. 
Published: June 2023

AbstractBackgroundWomen with lower extremity amputations (LEAs) tend to have poorer prosthesis‐related outcomes than men, although the literature is sparse. To our knowledge, there are no prior studies examining prosthesis‐related outcomes of women veterans with LEAs.ObjectiveTo examine gender differences (overall and by type of amputation) among veterans who underwent LEAs between 2005 and 2018, received care at the Veterans Health Administration (VHA) prior to undergoing amputation, and were prescribed a prosthesis. It was hypothesized that compared to men, women would report lower satisfaction with prosthetic services, poorer prosthesis fit, lower prosthesis satisfaction, less prosthesis use, and worse self‐reported mobility. Furthermore, it was hypothesized that gender differences in outcomes would be more pronounced among individuals with transfemoral than among those with transtibial amputations.DesignCross‐sectional survey. Linear regressions were used to assess overall gender differences in outcomes and gender differences based on type of amputation in a national sample of veterans.SettingVHA medical centers.ParticipantsThe sample consisted of 449 veterans who self‐identified their gender (women = 165, men = 284) with transtibial (n = 236), transfemoral (n = 135), and bilateral LEAs (n = 68) including all amputation etiologies.InterventionsNot applicable.Main Outcome MeasuresThe Orthotics and Prosthetics User's Survey, Trinity Amputation and Prosthesis Experiences Scale, and Prosthetic Limb Users Survey of Mobility‐Short Form were used to assess satisfaction with prosthetic services, prosthesis fit, prosthesis satisfaction, prosthesis use, and self‐reported mobility.ResultsWomen had poorer self‐reported mobility than men (d = −0.26, 95% confidence interval −0.49 to −0.02, p < .05); this difference was small. There were no statistically significant gender differences in satisfaction with prosthetic services, prosthesis fit, prosthesis satisfaction, daily hours of prosthesis use, or by amputation type.ConclusionsContrary to the hypothesis, prosthesis‐related outcomes were similar between men and women with LEAs. Minimal differences may in part be due to receiving care from the VHA's integrated Amputation System of Care.
Polyaniline modified waste‐derived graphene/sulfur nanocomposite cathode for lithium–sulfur batteries
Authors: Ponmani P. , Bahadur Jitendra , Tewari Chetna , Gupta Deepak Kumar , Kalita Uddhab , Jegadeesan P. , Ravindran T. R. , Alex Aby , Das Ashutosh , Sahoo Nandagopal , Sivanantham M. , Choudhury Soumyadip 
Published: June 2023

AbstractReduction of plastic wastes in the environment and solving the energy demands from renewable sources are two important challenging tasks of this century. Modern day lives are highly entangled with polymers, however handling the huge wastes from plastics is also a serious concern. Translating the plastic wastes to useful products such as graphene can be an alternative for nonbiodegradable polymer wastes. Efficient energy storage devices, for instance, batteries are required for storing the renewable energies. With the aim of regulating these issues, we report, for the first time, the preparation of high energy cathode materials from the nanocomposites (NCs) having polyaniline (PANI), waste‐derived graphene (WDG) derived from plastic waste and sulfur (S) for Li–S battery applications. We compare the electrochemical properties of cathodes derived from WDG/S and WDG/PANI/S in Li–S batteries. The specific discharge capacity of WDG/PANI/S at 0.1 C was obtained to be 880 mAhg−1 normalized to sulfur mass at 1st cycle, 472 mAhg−1 at 100th cycle, and 400 mAhg−1 at 160th cycle. The rate capability is also found to be good at C‐rates less than 0.5 C. We found that WDG/PANI/S showed decent electrochemical properties when compared with the reference sample, WDG/S at similar sulfur loading without PANI modification.
Zero‐Oil‐Fouling Membrane With High Coverage of Grafted Zwitterionic Polymer for Separation of Oil‐in‐Water Emulsions
Authors: Wang Aqiang , Zhu Yuzhang , Fang Wangxi , Gao Shoujian , Jin Jian 
Published: June 2023

AbstractCurrent hydrophilic modification strategies improve the antifouling ability of membranes but fail to completely eliminate the fouling of emulsified oil droplets with a wide size distribution. Constructing membranes with superior anti‐oil‐fouling ability to resist various oil droplets especially at high permeation fluxes is challenging. Here, the fabrication of a zero‐oil‐fouling membrane by grafting considerably high coverage of zwitterionic polymer and building defect‐free hydration defense barrier on the surface is reported. A uniform layer of protocatechuic acid with COOH as abundant as existing in every molecule is stably deposited on the membrane so as to provide sufficient reactive sites and achieve dense grafting of the zwitterionic polymer. The coverage of zwitterionic polymer on the membrane plays a crucial role in promoting the antifouling ability to emulsified oil droplets. The poly(vinylidene fluoride) membrane with 93% coverage of the zwitterionic polymer exhibits zero oil fouling when separating multitudinous oil‐in‐water emulsions with ≈0% flux decline, ≈100% flux recovery, and a high water flux of ≈800 L m−2 h−1 bar−1. This membrane outperforms almost all of the reported membranes in terms of the comprehensive antifouling performance. This work provides a feasible route for manufacturing super‐antifouling membranes toward oil/water separation application.
Enantiomeric separation of ketoprofen using Frovatriptan as a chiral derivatizing reagent
Authors: Vashistha Vinod Kumar , Bala Renu , Pullabhotla Rajasekhar VSR , Mittal Ankit 
Published: June 2023

AbstractWe established a validated high‐performance liquid chromatography method utilizing Frovatriptan as a chiral derivatizing agent to separate the enantiomers of ketoprofen from commercially available tablet formulations. The chiral derivatizing agent facilitated the synthesis of diastereomeric derivatives of ketoprofen, which were separated on a reversed‐phase C18 column using a mobile phase composed of acetonitrile and 0.1% trifluoroacetic acid under gradient elution from 30% to 70% of acetonitrile. The method demonstrated a limit of detection and quantitation of 2.52 and 8.31 ng/ml for the derivatives, respectively. Validation of the method was performed following the guidelines set by the International Council for Harmonisation to ensure accuracy, precision, and reliability, making it suitable for pharmaceutical industry applications. Notably, this study introduces the novel application of Frovatriptan as a chiral derivatizing agent in high‐performance liquid chromatography for the separation of ketoprofen enantiomers.
Alignment Through Value Consolidation Mechanisms—Focusing on Multi-Stakeholder Collaboration for Circular Economy
Authors: Vikstedt Elina , Rajala Tomi 
Published: June 2023

AbstractThe role of multistakeholder collaboration in solving complex sustainability challenges is emphasised across academic literature. Stakeholders from different sectors typically adhere to different institutional logics and create value in different ways. Alignment reflects how stakeholders orient themselves towards multiple institutional logics in collaborative value creation. By reviewing the extant research literature, we identify the value-consolidation mechanisms employed in aligning institutional logics in stakeholder engagement. We empirically examine these mechanisms in three key dimensions: stakeholders’ cognition, goals, and practices. Two multistakeholder collaborations for circular economy were selected for the case study. After collecting interviews and document data and conducting qualitative content analysis, we found that several value-consolidation mechanisms were applied side by side in dynamic ways to align the cognition, goals, and practices of the stakeholders. Findings indicate that perfect alignment in multistakeholder settings may be hard to reach, but this does not always hinder collaborative value creation. The proposed framework offers alignment instruments for practitioners pursuing stakeholder engagement in collaborative value creation. Scholars can use the framework to determine which conditions incite the use of certain value-consolidation mechanisms, as well as what kinds of impacts this has on collaborative value creation.
Thoughts about fertility among female adolescents and young adults with cancer: a qualitative study
Authors: Bentsen Line , Pappot Helle , Hjerming Maiken , Hanghøj Signe 
Published: June 2023

Abstract
Purpose
Nine hundred female adolescents and young adults (AYAs) aged 15–39 are diagnosed with cancer in Denmark annually. Advances in cancer therapy have led to increased long-term survival; however, a serious side effect of cancer therapy is reduced fertility. The aim of our study was to explore the thoughts about fertility among female AYAs with cancer.

Methods
Our study was conducted from September 2020 to March 2021 at the Copenhagen University Hospital, Rigshospitalet. Inclusion criteria were female AYAs with cancer aged 18–39. Twelve individual, semi-structured, qualitative interviews were performed with female AYAs with cancer (20–35 years). Data were analysed using thematic analysis.

Results
Four main themes were found: (1) the female AYAs held on to a hope of having children in the future; (2) the female AYAs experienced time pressure and waiting time as a sprint as well as a marathon; (3) the female AYAs faced existential and ethical choices about survival and family formation; and (4) the female AYAs felt a loss of control of their bodies.

Conclusion
Our study contributes with knowledge on how important holding on to the hope of children in the future is among female AYAs with cancer. Meanwhile, they are frustrated by the rushed decision on fertility preservation at diagnosis. The female AYAs also have existential and ethical concerns related to the choice of cancer therapy and fertility preservation. Finally, they suffer from altered body image, loss of femininity, and body control due to hormone therapy.
Anti-quasi-Sasakian manifolds
Authors: Di Pinto D. , Dileo G. 
Published: June 2023

AbstractWe introduce and study a special class of almost contact metric manifolds, which we call anti-quasi-Sasakian (aqS). Among the class of transversely Kähler almost contact metric manifolds $$(M,\varphi , \xi ,\eta ,g)$$

(
M
,
φ
,
ξ
,
η
,
g
)

, quasi-Sasakian and anti-quasi-Sasakian manifolds are characterized, respectively, by the $$\varphi $$
φ
-invariance and the $$\varphi $$
φ
-anti-invariance of the 2-form $$\textrm{d}\eta $$

d
η

. A Boothby–Wang type theorem allows to obtain aqS structures on principal circle bundles over Kähler manifolds endowed with a closed (2, 0)-form. We characterize aqS manifolds with constant $$\xi $$
ξ
-sectional curvature equal to 1: they admit an $$Sp(n)\times 1$$

S
p
(
n
)
×
1

-reduction of the frame bundle such that the manifold is transversely hyperkähler, carrying a second aqS structure and a null Sasakian $$\eta $$
η
-Einstein structure. We show that aqS manifolds with constant sectional curvature are necessarily flat and cokähler. Finally, by using a metric connection with torsion, we provide a sufficient condition for an aqS manifold to be locally decomposable as the Riemannian product of a Kähler manifold and an aqS manifold with structure of maximal rank. Under the same hypothesis, (M, g) cannot be locally symmetric.