bluuebunny/crossref_metadata_2025_split huggingface dataset:
A precise forest spatial structure investigation using the SLAM+AR technology
Authors: Yan Fei , Guan Tianshuo , Ullah Mohammad Rahmat , Gao Li , Fan Yongxiang 
Published: June 2023

IntroductionForest spatial structures are the foundations of the structure and function of forest ecosystems. Quantitative descriptions and analyses of forest spatial structure have recently become common tools for digitalized forest management. Therefore, the accuracy and intelligence of acquiring forest spatial structure information are of great significance.MethodsIn this study, we developed a forest measurement system using a mobile phone. Through this system, the following tree measurements can be achieved: (1) point cloud of tree and chest diameter circle to measure tree diameter at breast height (DBH) and position coordinates of tree by using simultaneous localization and mapping (SLAM) technology, (2) virtual boundary creation of the sample plot, and the auxiliary measurement function of tree with the augmented reality (AR) interactive module, and (3) position coordinates and single-tree volume factor to calculate the spatial structural parameters of the forest (e.g., Mingling degree, Dominance index, Uniform angle index, and Crowdedness index).The system was tested in three 32 x 32 martificial forest plots.ResultsThe average DBH estimations showed BIAS of -0.47 to 0.45 cm and RMSEs of 0.57 to 0.95 cm. Its accuracy level met the requirements of forestry sample surveys. The tree position estimates for the three plots had relatively small RMSEs with 0.17 to 0.22 m on the x-axis and 0.16 to 0.26 m on the y-axis. The spatial structural parameters were as follows: the mingling degree of plot 1 was 0.32, and the overall mixing degree of tree species was low. The trees in plots 2 and 3 were all single species, and the mixing degree of both plots was 0. The dominance index of the three plots was 0.56, 0.51, and 0.51, indicating that the competitive advantage of the whole orest species was not obvious. The uniform angle index of the three plots was 0.55, 0.59, and 0.61, indicating that the positions of trees in the three plots were randomly distributed. The crowdedness index of plot 1 was 1.03, indicating that the degree of aggregation of the trees was low and showed a random distribution trend. The crowdedness index of the other plots were 1.36 and 1.40, indicating that the trees in the plots show a trend of uniform distribution, and the uniformity of plot 3 is higher than that of plot 2, but the overall uniformity is relatively weak.DiscussionThe findings of this study provide support for the optimization of forest structures and improve our conceptual understanding of forest community succession and restoration, in addition to the informatization and precision of forest spatial structure surveys.
Uniparental disomy for chromosome 1 with POMGNT1 splice-site variant causes muscle-eye-brain disease
Authors: Liu Yi-Dan , Tan Dan-Dan , Song Dan-Yu , Fan Yan-Bin , Fu Xiao-Na , Ge Lin , Wei Wei , Xiong Hui 
Published: June 2023

POMGNT1, encoding protein O-mannose beta-1,2-N-acetylglucosaminyltransferase 1, is one of the genes responsible for dystroglycanopathy (DGP), which includes multiple phenotypes such as muscle-eye-brain disease (MEB), congenital muscular dystrophy with intellectual disability, and limb-girdle muscular dystrophy Here, we report a case of MEB that is the result of a homozygous variant of POMGNT1 that is revealed through uniparental disomy (UPD). An 8-month-old boy was admitted with mental and motor retardation, hypotonia, esotropia, early onset severe myopia, and structural brain abnormalities. A panel testing of genetic myopathy-related genes was used to identify a homozygous c.636C&gt;T (p.Phe212Phe) variant in exon 7 of POMGNT1 in the patient, a heterozygous c.636C&gt;T variant in the father, and the wild type in the mother. Quantitative polymerase chain reaction (q-PCR) revealed no abnormal copy numbers in exon 7. Trio-based whole-exome sequencing (trio-WES) revealed a possible paternal UPD on chromosome 1 of the patient. Chromosomal microarray analysis (CMA) revealed a 120,451 kb loss of heterozygosity (LOH) on 1p36.33-p11.2, encompassing POMGNT1, and a 99,319 kb loss of heterozygosity on 1q21.2-q44, which indicated UPD. Moreover, RNA sequencing (RNA-seq) verified that the c.636C&gt;T variant was a splice-site variant, leading to skipping of exon 7 (p.Asp179Valfs*23). In conclusion, to the best of our knowledge, we present the first case of MEB caused by UPD, providing valuable insights into the genetic mechanisms underlying this condition.
Severe respiratory distress secondary to pharyngeal perforation during endoscopic gastrostomy tube removal: a clinical case report
Authors: Siblani Dima , Stiel Laure , Husson-Wetzel Stéphanie , Barsotti Pierre 
Published: June 2023

We present the case of a 60-year-old patient with advanced chronic obstructive pulmonary disease (COPD), who presented for planned endoscopic removal of her gastrostomy feeding tube, which was inserted for nutritional status optimization prior to lung transplantation. The procedure was complicated by accidental blockage of the device at the pharyngeal level, causing a transmural laceration. Rapid respiratory distress developed with subcutaneous emphysema that led to the intubation of the patient. A new endoscopic retrieval was attempted but failed, and the patient was sent to the operating room after a cervical and thoracic CT scan that showed the blocked piece in the cervical wall, in addition to diffuse subcutaneous emphysema, a large pneumomediastinum, and a left pneumothorax. The surgery consisted of a left cervicotomy, a pharyngeal incision, and retrieval of the trapped parts. The patient was sent to the intensive care unit (ICU) where she could be weaned and extubated 1 week later.
A review of the main genetic factors influencing the course of COVID-19 in Sardinia: the role of human leukocyte antigen-G
Authors: Mocci Stefano , Littera Roberto , Chessa Luchino , Campagna Marcello , Melis Maurizio , Ottelio Carla Maria , Piras Ignazio S. , Lai Sara , Firinu Davide , Tranquilli Stefania , Mascia Alessia , Vacca Monica , Schirru Daniele , Lecca Luigi Isaia , Rassu Stefania , Cannas Federica , Sanna Celeste , Carta Mauro Giovanni , Sedda Francesca , Giuressi Erika , Cipri Selene , Miglianti Michela , Perra Andrea , Giglio Sabrina 
Published: June 2023

IntroductionA large number of risk and protective factors have been identified during the SARS-CoV-2 pandemic which may influence the outcome of COVID-19. Among these, recent studies have explored the role of HLA-G molecules and their immunomodulatory effects in COVID-19, but there are very few reports exploring the genetic basis of these manifestations. The present study aims to investigate how host genetic factors, including HLA-G gene polymorphisms and sHLA-G, can affect SARS-CoV-2 infection.Materials and MethodsWe compared the immune-genetic and phenotypic characteristics between COVID-19 patients (n = 381) with varying degrees of severity of the disease and 420 healthy controls from Sardinia (Italy).ResultsHLA-G locus analysis showed that the extended haplotype HLA-G*01:01:01:01/UTR-1 was more prevalent in both COVID-19 patients and controls. In particular, this extended haplotype was more common among patients with mild symptoms than those with severe symptoms [22.7% vs 15.7%, OR = 0.634 (95% CI 0.440 – 0.913); P = 0.016]. Furthermore, the most significant HLA-G 3’UTR polymorphism (rs371194629) shows that the HLA-G 3’UTR Del/Del genotype frequency decreases gradually from 27.6% in paucisymptomatic patients to 15.9% in patients with severe symptoms (X2 = 7.095, P = 0.029), reaching the lowest frequency (7.0%) in ICU patients (X2 = 11.257, P = 0.004). However, no significant differences were observed for the soluble HLA-G levels in patients and controls. Finally, we showed that SARS-CoV-2 infection in the Sardinian population is also influenced by other genetic factors such as β-thalassemia trait (rs11549407C&gt;T in the HBB gene), KIR2DS2/HLA-C C1+ group combination and the HLA-B*58:01, C*07:01, DRB1*03:01 haplotype which exert a protective effect [P = 0.005, P = 0.001 and P = 0.026 respectively]. Conversely, the Neanderthal LZTFL1 gene variant (rs35044562A&gt;G) shows a detrimental consequence on the disease course [P = 0.001]. However, by using a logistic regression model, HLA-G 3’UTR Del/Del genotype was independent from the other significant variables [ORM = 0.4 (95% CI 0.2 – 0.7), PM = 6.5 x 10-4].ConclusionOur results reveal novel genetic variants which could potentially serve as biomarkers for disease prognosis and treatment, highlighting the importance of considering genetic factors in the management of COVID-19 patients.
Pregnancy-associated systemic gene expression compared to a pre-pregnancy baseline, among healthy women with term pregnancies
Authors: Wright Matthew L. , Goin Dana E. , Smed Mette Kiel , Jewell Nicholas P. , Nelson J. Lee , Olsen Jørn , Hetland Merete Lund , Zoffmann Vibeke , Jawaheer Damini 
Published: June 2023

BackgroundPregnancy is known to induce extensive biological changes in the healthy mother. Little is known, however, about what these changes are at the molecular level. We have examined systemic expression changes in protein-coding genes and long non-coding (lnc) RNAs during and after pregnancy, compared to before pregnancy, among healthy women with term pregnancies.MethodsBlood samples were collected from 14 healthy women enrolled in our prospective pregnancy cohort at 7 time-points (before, during and after pregnancy). Total RNA from frozen whole blood was used for RNA sequencing. Following raw read alignment and assembly, gene-level counts were obtained for protein-coding genes and long non-coding RNAs. At each time-point, cell type proportions were estimated using deconvolution. To examine associations between pregnancy status and gene expression over time, Generalized Estimating Equation (GEE) models were fitted, adjusting for age at conception, and with and without adjusting for changes in cell type proportions. Fold-changes in expression at each trimester were examined relative to the pre-pregnancy baseline.ResultsNumerous immune-related genes demonstrated pregnancy-associated expression, in a time-dependent manner. The genes that demonstrated the largest changes in expression included several that were neutrophil-related (over-expressed) and numerous immunoglobulin genes (under-expressed). Estimated cell proportions revealed a marked increase in neutrophils, and less so of activated CD4 memory T cells, during pregnancy, while most other cell type proportions decreased or remained unchanged. Adjusting for cell type proportions in our model revealed that although most of the expression changes were due to changes in cell type proportions in the bloodstream, transcriptional regulation was also involved, especially in down-regulating expression of type I interferon inducible genes.ConclusionCompared to a pre-pregnancy baseline, there were extensive systemic changes in cell type proportions, gene expression and biological pathways associated with different stages of pregnancy and postpartum among healthy women. Some were due to changes in cell type proportions and some due to gene regulation. In addition to providing insight into term pregnancy among healthy women, these findings also provide a “normal” reference for abnormal pregnancies and for autoimmune diseases that improve or worsen during pregnancy, to assess deviations from normal.
Dysregulated peripheral proteome reveals NASH-specific signatures identifying patient subgroups with distinct liver biology
Authors: Stiglund Natalie , Hagström Hannes , Stål Per , Cornillet Martin , Björkström Niklas K. 
Published: June 2023

Background and aimsNon-alcoholic fatty liver disease (NAFLD) is the most common chronic liver disease. The prognosis may vary from simple steatosis to more severe outcomes such as nonalcoholic steatohepatitis (NASH), liver cirrhosis, and hepatocellular carcinoma. The understanding of the biological processes leading to NASH is limited and non-invasive diagnostic tools are lacking.MethodsThe peripheral immunoproteome in biopsy-proven NAFL (n=35) and NASH patients (n=35) compared to matched, normal-weight healthy controls (n=15) was studied using a proximity extension assay, combined with spatial and single cell hepatic transcriptome analysis.ResultsWe identified 13 inflammatory serum proteins that, independent of comorbidities and fibrosis stage, distinguished NASH from NAFL. Analysis of co-expression patterns and biological networks further revealed NASH-specific biological perturbations indicative of temporal dysregulation of IL-4/-13, -10, -18, and non-canonical NF-kβ signaling. Of the identified inflammatory serum proteins, IL-18 and EN-RAGE as well as ST1A1 mapped to hepatic macrophages and periportal hepatocytes, respectively, at the single cell level. The signature of inflammatory serum proteins further permitted identification of biologically distinct subgroups of NASH patients.ConclusionNASH patients have a distinct inflammatory serum protein signature, which can be mapped to the liver parenchyma, disease pathogenesis, and identifies subgroups of NASH patients with altered liver biology.
Thoracolumbar fascia deformation during deadlifting and trunk extension in individuals with and without back pain
Authors: Brandl Andreas , Wilke Jan , Egner Christoph , Reer Rüdiger , Schmidt Tobias , Schleip Robert 
Published: June 2023

BackgroundAlterations in posture, lumbopelvic kinematics, and movement patterns are commonly seen in patients with low back pain. Therefore, strengthening the posterior muscle chain has been shown to result in significant improvement in pain and disability status. Recent studies suggest that thoracolumbar fascia (TLF) has a major impact on the maintenance of spinal stability and paraspinal muscle activity, and thus is likely to have an equal impact on deadlift performance.ObjectiveAim of the study was to evaluate the role of thoracolumbar fascia deformation (TFLD) during spinal movement in track and field athletes (TF) as well as individuals with and without acute low back pain (aLBP).MethodsA case–control study was performed with n = 16 aLBP patients (cases) and two control groups: untrained healthy individuals (UH, n = 16) and TF (n = 16). Participants performed a trunk extension task (TET) and a deadlift, being assessed for erector spinae muscle thickness (EST) and TLFD using high-resolution ultrasound imaging. Mean deadlift velocity (VEL) and deviation of barbell path (DEV) were measured by means of a three-axis gyroscope. Group differences for TLFD during the TET were examined using ANOVA. Partial Spearman rank correlations were calculated between TLFD and VEL adjusting for baseline covariates, EST, and DEV. TLFD during deadlifting was compared between groups using ANCOVA adjusting for EST, DEV, and VEL.ResultsTLFD during the TET differed significantly between groups. TF had the largest TLFD (−37.6%), followed by UH (−26.4%), while aLBP patients had almost no TLFD (−2.7%). There was a strong negative correlation between TLFD and deadlift VEL in all groups (r = −0.65 to −0.89) which was highest for TF (r = −0.89). TLFD during deadlift, corrected for VEL, also differed significantly between groups. TF exhibited the smallest TLFD (−11.9%), followed by aLBP patients (−21.4%), and UH (−31.9%).ConclusionTFLD maybe a suitable parameter to distinguish LBP patients and healthy individuals during lifting tasks. The cause-effect triangle between spinal movement, TFLD and movement velocity needs to be further clarified.Clinical trial registrationhttps://drks.de/register/de/trial/DRKS00027074/, German Clinical Trials Register DRKS00027074.
Genomic New Insights Into Emergence and Clinical Therapy of Multidrug-Resistant Klebsiella pneumoniae in Infected Pancreatic Necrosis
Authors: Hao Haibin , Liu Yang , Cao Jin , Gao Kun , Lu Yingying , Wang Weiping , Wang Peng , Lu Sida , Hu Long , Tong Zhihui , Li Weiqin 
Published: June 2023

Graphical AbstractBased on the genotype and phenotype, aztreonam plus avibactam were considered for multi-drug resistant Klebsiella pneumoniae in infected pancreatic necrosis.
Red blood cell distribution width combined with age as a predictor of acute ischemic stroke in stable COPD patients
Authors: Cai Shikun , Li Yao , Sun Bo , Wang Kai , Wan Zongren , Yang Dan , Tian Xiangyang , Wu Liao , Zhu Rong 
Published: June 2023

AimThis retrospective study aimed to investigate the independent clinical variables associated with the onset of acute cerebral ischemic stroke (AIS) in patients with stable chronic obstructive pulmonary disease (COPD).MethodA total of 244 patients with COPD who had not experienced a relapse within 6 months were included in this retrospective study. Of these, 94 patients hospitalized with AIS were enrolled in the study group, and the remaining 150 were enrolled in the control group. Clinical data and laboratory parameters were collected for both groups within 24 h after hospitalization, and the data of the two groups were statistically analyzed.ResultsThe levels of age, white blood cell (WBC), neutrophil (NEUT), glucose (GLU), prothrombin time (PT), albumin (ALB), and red blood cell distribution width (RDW) were different in the two groups (P &lt; 0.01). Logistic regression analysis showed that age, WBC, RDW, PT, and GLU were independent risk factors for the occurrence of AIS in patients with stable COPD. Age and RDW were selected as new predictors, and the receiver operating characteristic curves (ROC) were plotted accordingly. The areas under the ROC curves of age, RDW, and age + RDW were 0.7122, 0.7184, and 0.7852, respectively. The sensitivity was 60.5, 59.6, and 70.2%, and the specificity was 72.4, 86.0, and 60.0%, respectively.ConclusionThe combination of RDW and age in patients with stable COPD might be a potential predictor for the onset of AIS.
Postural threat increases sample entropy of postural control
Authors: Fischer Olivia M. , Missen Kyle J. , Tokuno Craig D. , Carpenter Mark G. , Adkin Allan L. 
Published: June 2023

IntroductionPostural threat elicits modifications to standing balance. However, the underlying neural mechanism(s) responsible remain unclear. Shifts in attention focus including directing more attention to balance when threatened may contribute to the balance changes. Sample entropy, a measure of postural sway regularity with lower values reflecting less automatic and more conscious control of balance, may support attention to balance as a mechanism to explain threat-induced balance changes. The main objectives were to investigate the effects of postural threat on sample entropy, and the relationships between threat-induced changes in physiological arousal, perceived anxiety, attention focus, sample entropy, and traditional balance measures. A secondary objective was to explore if biological sex influenced these relationships.MethodsHealthy young adults (63 females, 42 males) stood quietly on a force plate without (No Threat) and with (Threat) the expectation of receiving a postural perturbation (i.e., forward/backward support surface translation). Mean electrodermal activity and anterior–posterior centre of pressure (COP) sample entropy, mean position, root mean square, mean power frequency, and power within low (0–0.05 Hz), medium (0.5–1.8 Hz), and high-frequency (1.8–5 Hz) components were calculated for each trial. Perceived anxiety and attention focus to balance, task objectives, threat-related stimuli, self-regulatory strategies, and task-irrelevant information were rated after each trial.Results and DiscussionSignificant threat effects were observed for all measures, except low-frequency sway. Participants were more physiologically aroused, more anxious, and directed more attention to balance, task objectives, threat-related stimuli, and self-regulatory strategies, and less to task-irrelevant information in the Threat compared to No Threat condition. Participants also increased sample entropy, leaned further forward, and increased the amplitude and frequency of COP displacements, including medium and high-frequency sway, when threatened. Males and females responded in the same way when threatened, except males had significantly larger threat-induced increases in attention to balance and high-frequency sway. A combination of sex and threat-induced changes in physiological arousal, perceived anxiety, and attention focus accounted for threat-induced changes in specific traditional balance measures, but not sample entropy. Increased sample entropy when threatened may reflect a shift to more automatic control. Directing more conscious control to balance when threatened may act to constrain these threat-induced automatic changes to balance.
The role of high-sensitivity C-reactive protein serum levels in the prognosis for patients with stroke: a meta-analysis
Authors: Chen Liuting , Wang Min , Yang Chanrui , Wang Yefei , Hou Bonan 
Published: June 2023

BackgroundThe impact of high-sensitivity C-reactive protein (hs-CRP) as a biomarker of inflammation on the prognosis of stroke patients remains controversial, this study was conducted to evaluate the prognostic value of hs-CRP levels for patients with stroke.MethodsPubMed, Web of Science, Embase, and Cochrane Library databases were searched from inception to October 28, 2022. Outcome measures were all-cause mortality, recurrent stroke, and poor prognosis. The relationship between the highest versus lowest levels of hs-CRP or per unit increment and outcomes as measured by risk ratio (RR) and corresponding 95% confidence intervals (CI).ResultsA total of 39 articles were eligible for meta-analysis. High hs-CRP levels at admission were associated with mortality among patients with acute ischemic stroke (AIS) [RR = 3.84, 95% CI (2.41 ~ 6.111); p &lt; 0.001], risk of recurrent stroke [RR = 1.88, 95%CI (1.41 ~ 2.52); p &lt; 0.001], and poor prognosis [RR = 1.77, 95% CI (1.59 ~ 1.97); p &lt; 0.001]. The risk ratios for the association of per unit increase in hs-CRP levels with mortality, risk of recurrent stroke, and poor prognosis were as follows, respectively: 1.42 [95% CI (1.19–1.69); p &lt; 0.001], 1.03 [95% CI (1.01–1.04); p = 0.003], and 1.27 [95% CI (1.10–1.47); p = 0.001]. For hemorrhagic stroke (HS), the risk ratios (RR) for the highest versus the lowest (reference) category of hsCRP or per unit increment to all-cause mortality were 4.36 [95% CI (1.38–13.73); p = 0.012] and 1.03 [95% CI (0.98–1.08); p = 0.238].ConclusionHs-CRP levels are strongly associated with mortality, risk of stroke recurrence and poor prognosis in stroke patients. Therefore, hs-CRP levels may contribute to the prognosis prediction of these patients.