bluuebunny/crossref_metadata_2025_split huggingface dataset:
MAM-2201 acute administration impairs motor, sensorimotor, prepulse inhibition, and memory functions in mice: a comparison with its analogue AM-2201
Authors: Corli Giorgia , Tirri Micaela , Bilel Sabrine , Arfè Raffaella , Coccini Teresa , Roda Elisa , Marchetti Beatrice , Vincenzi Fabrizio , Zauli Giorgio , Borea Pier Andrea , Locatelli Carlo Alessandro , Varani Katia , Marti Matteo 
Published: May 2023

Abstract
Rationale
1-[(5-fluoropentyl)-1H-indol-3-yl](4-methyl-1-naphthalenyl) methanone (MAM-2201) is a potent synthetic cannabinoid receptor agonist illegally marketed in “spice” products and as “synthacaine” for its psychoactive effects. It is a naphthoyl-indole derivative which differs from its analogue 1-[(5-Fluoropentyl)-1H-indol-3-yl](1-naphthylenyl) methanone (AM-2201) by the presence of a methyl substituent on carbon 4 (C-4) of the naphthoyl moiety. Multiple cases of intoxication and impaired driving have been linked to AM-2201 and MAM-2201 consumption.

Objectives
This study aims to investigate the in vitro (murine and human cannabinoid receptors) and in vivo (CD-1 male mice) pharmacodynamic activity of MAM-2201 and compare its effects with those induced by its desmethylated analogue, AM-2201.

Results
In vitro competition binding studies confirmed that MAM-2201 and AM-2201 possess nanomolar affinity for both CD-1 murine and human CB1 and CB2 receptors, with preference for the CB1 receptor. In agreement with the in vitro binding data, in vivo studies showed that MAM-2201 induces visual, acoustic, and tactile impairments that were fully prevented by pretreatment with CB1 receptor antagonist/partial agonist AM-251, indicating a CB1 receptor mediated mechanism of action. Administration of MAM-2201 also altered locomotor activity and PPI responses of mice, pointing out its detrimental effect on motor and sensory gating functions and confirming its potential use liability. MAM-2201 and AM-2201 also caused deficits in short- and long-term working memory.

Conclusion
These findings point to the potential public health burden that these synthetic cannabinoids may pose, with particular emphasis on impaired driving and workplace performance.
Lethal and Sub-lethal Implications of Sodium Chloride Exposure for Adult Unionid Mussel Species: Eurynia dilatata and Lasmigona costata
Authors: Burton Erika A. , Atkinson Brian , Salerno Joseph , Khan Hufsa N. , Prosser Ryan S. , Gillis Patricia L. 
Published: May 2023

AbstractThe elevated use of salt as a de-icing agent on roads in Canada is causing an increase in the chloride concentration of freshwater ecosystems. Freshwater Unionid mussels are a group of organisms that are sensitive to increases in chloride levels. Unionids have greater diversity in North America than anywhere else on Earth, but they are also one of the most imperiled groups of organisms. This underscores the importance of understanding the effect that increasing salt exposure has on these threatened species. There are more data on the acute toxicity of chloride to Unionids than on chronic toxicity. This study investigated the effect of chronic sodium chloride exposure on the survival and filtering activity of two Unionid species (Eurynia dilatata, and Lasmigona costata) and assessed the effect on the metabolome in L. costata hemolymph. The concentration causing mortality after 28 days of exposure was similar for E. dilatata (1893 mg Cl−/L) and L. costata (1903 mg Cl−/L). Significant changes in the metabolome of the L. costata hemolymph were observed for mussels exposed to non-lethal concentrations. For example, several phosphatidylethanolamines, several hydroxyeicosatetraenoic acids, pyropheophorbide-a, and alpha-linolenic acid were significantly upregulated in the hemolymph of mussels exposed to 1000 mg Cl−/L for 28 days. While no mortality occurred in the treatment, elevated metabolites in the hemolymph are an indicator of stress.
Association between routine cell salvage use for lower segment caesarean section and post-operative iron infusion and anemia
Authors: Fox Tom P. , Timpani Evelyn , Green Amanda , Parange Anupam , Sinha Romi , Kelly Thu-Lan , Hodyl Nicolette A. , Froessler Bernd 
Published: May 2023

Abstract
Purpose
Intraoperative cell salvage is central to Patient Blood Management including for lower segment caesarean section. Prior to April 2020, we initiated intraoperative cell salvage during caesarean section based on risk assessment for hemorrhage and patient factors. As the pandemic broadened, we mandated intraoperative cell salvage to prevent peri-partum anemia and potentially reduce blood product usage. We examined the association of routine intraoperative cell salvage on maternal outcomes.

Methods
We conducted a single-center non-overlapping before-after study of obstetric patients undergoing lower segment caesarean section in the 2 months prior to a change in practice (‘usual care = selective intraoperative cell salvage’, n = 203) and the 2 months following (‘mandated intraoperative cell salvage’, n = 228). Recovered blood was processed when a minimal autologous reinfusion volume of 100 ml was expected. Post-operative iron infusion and length of stay were modelled using logistic or linear regression, using inverse probability weighting to account for confounding.

Results
More emergency lower-segment caesarean sections occurred in the Usual Care group. Compared to the Usual Care group, post-operative hemoglobin was higher and anemia cases fewer in the Mandated intraoperative cell salvage group. Rates of post-partum iron infusion were significantly lower in the Mandated intraoperative cell salvage group (OR = 0.31, 95% CI = 0.12 to 0.80, P = 0.016). No difference was found for length of stay.

Conclusion
Routine cell salvage provision during lower segment caesarean section was associated with a significant reduction in post-partum iron infusions, increased post-operative hemoglobin and reduced anemia prevalence.
Apicomplexan parasites are attenuated by low-energy electron irradiation in an automated microfluidic system and protect against infection with Toxoplasma gondii
Authors: Finkensieper Julia , Mayerle Florian , Rentería-Solís Zaida , Fertey Jasmin , Makert Gustavo R. , Lange Franziska , Besecke Joana , Schopf Simone , Poremba Andre , König Ulla , Standfest Bastian , Thoma Martin , Daugschies Arwid , Ulbert Sebastian 
Published: May 2023

AbstractRadiation-attenuated intracellular parasites are promising immunization strategies. The irradiated parasites are able to invade host cells but fail to fully replicate, which allows for the generation of an efficient immune response. Available radiation technologies such as gamma rays require complex shielding constructions and are difficult to be integrated into pharmaceutical production processes. In this study, we evaluated for the first time low-energy electron irradiation (LEEI) as a method to generate replication-deficient Toxoplasma gondii and Cryptosporidium parvum. Similar to other radiation technologies, LEEI mainly damages nucleic acids; however, it is applicable in standard laboratories. By using a novel, continuous, and microfluidic-based LEEI process, tachyzoites of T. gondii and oocysts of C. parvum were irradiated and subsequently analyzed in vitro. The LEEI-treated parasites invaded host cells but were arrested in intracellular replication. Antibody-based analysis of surface proteins revealed no significant structural damage due to LEEI. Similarly, excystation rates of sporozoites from irradiated C. parvum oocysts were similar to those from untreated controls. Upon immunization of mice, LEEI-attenuated T. gondii tachyzoites induced high levels of antibodies and protected the animals from acute infection. These results suggest that LEEI is a useful technology for the generation of attenuated Apicomplexan parasites and has potential for the development of anti-parasitic vaccines.
Psychological and social challenges of patients with locally advanced and metastatic gastrointestinal stromal tumours (GIST) on long-term treatment with tyrosine kinase inhibitors: a qualitative study with patients and medical oncologists
Authors: van de Wal Deborah , Fauske Lena , Bruland Øyvind S. , Jones Robin L. , Kasper Bernd , Wilson Roger , van der Graaf Winette T. A. , Husson Olga 
Published: May 2023

Abstract
Purpose
Tyrosine kinase inhibitors (TKIs) have revolutionized the treatment of locally advanced and metastatic gastrointestinal stromal tumours (GISTs). Patients are experiencing prolonged survival but often at the expense of their health-related quality of life. It is not only the physical side effects that impact GIST patients’ daily lives but also the psychological and social challenges they have to deal with. This qualitative study aimed to explore the psychological and social life challenges of GIST patients with locally advanced and metastatic disease on ≥ 5 years TKI treatment.

Methods
Semi-structured interviews with 15 locally advanced and/or metastatic GIST patients and 10 medical oncologists with experience of delivering care to this specific patient group were conducted. Thematic analysis was used to interpret the data.

Results
Psychological challenges expressed by participants concerned fears, scanxiety, negative change in emotion and mood, doubts about their treatment and follow-up, living with uncertainty, lack of understanding from others or healthcare professionals, and constantly being reminded of their illness. Challenges regarding social health included financial difficulties, challenges in relationships, concerns about fertility and parenting, work, and impact on social activities.

Conclusion
The reported psychological and social challenges can significantly hamper the overall quality of life of GIST patients. Some challenges were clearly underreported and hardly recognized by medical oncologist, as they may tend to focus on the physical side effects and clinical outcomes of treatment. Therefore, it is essential to take the patient’s perspective into account in research and clinical practice to ensure optimal care for this patient group.
Primary succession of ectomycorrhizal fungi associated with Alnus sieboldiana on Izu-Oshima Island, Japan
Authors: Ishikawa Akira , Nara Kazuhide 
Published: May 2023

AbstractThe primary succession of ectomycorrhizal (ECM) fungi has been well described for Pinus and Salix, but the succession for other pioneer hosts is almost unknown. Here, we investigated ECM fungal communities of Alnus sieboldiana at different host growth stages in a primary successional volcanic site on Izu-Oshima Island, Japan. ECM root tips were collected from 120 host individuals, encompassing seedling, sapling, and mature tree stages. The taxonomic identity of the ECM fungi was determined based on rDNA internal transcribed spacer region sequences. Nine molecular taxonomic units were detected from a total of 807 root tips. The initial ECM fungal community on the pioneer seedlings was composed of only three species, where an undescribed Alpova species (Alpova sp.) was exclusively frequent. With host growth, other ECM fungal species were added to the communities, while the initial colonizers remained even at mature tree stages. Thus, the ECM fungal composition significantly changed along host growth stages and showed the nested community structure. Although most of the ECM fungi confirmed in this study had a broad Holarctic geographical distribution, the Alpova sp. had no previous records in other regions. These results suggest that a locally evolved Alpova sp. plays an essential role in the initial seedling establishment of A. sieboldiana at early successional volcanic sites.
Level of difficulty of tooth extractions among roughly 100,000 procedures in primary care
Authors: Lindahl Oona , Ventä Irja 
Published: May 2023

Abstract
Objectives
The study examined treatment codes of extracted teeth and aimed to assess degree of difficulty concerning all tooth extractions.

Materials and methods
Retrospective data on treatment codes of all tooth extractions during a two-year period were obtained from the patient register in primary oral healthcare of the City of Helsinki, Finland. Prevalence, indication, and method of extraction appeared in the treatment codes (EBA-codes). Degree of difficulty was determined from the method and classified as non-operative or operative and as routine or demanding. Statistics included frequencies, percentages, and χ2 test.

Results
Total number of extraction procedures was 97,276, including 121,342 extracted teeth. The most frequent procedure was a routine extraction of a tooth with forceps (55%, n = 53,642). The main reason for extraction was caries (27%, n = 20,889). Of the extractions, 79% (n = 76,435) were non-operative, 13% (n = 12,819) operative, and 8% (n = 8,022) multiple extractions in one visit. Level of difficulty was distributed as routine non-operative (63%), demanding non-operative (15%), routine operative (12%), demanding operative (2%), and multiple extractions (8%).

Conclusions
Two-thirds of all tooth extractions in primary care were relatively simple. However, 29% of procedures were classified as demanding.

Clinical relevance
As earlier methods for assessing level of difficulty were aimed at third molars alone, an analysis was presented for all tooth extractions. This approach may be useful for research purposes, and the profile of tooth extractions and their difficulty level may be practical also for decision-makers in primary care.
A step-by-step researcher's guide to the use of an AI-based transformer in epidemiology: an exploratory analysis of ChatGPT using the STROBE checklist for observational studies
Authors: Sanmarchi Francesco , Bucci Andrea , Nuzzolese Andrea Giovanni , Carullo Gherardo , Toscano Fabrizio , Nante Nicola , Golinelli Davide 
Published: May 2023

Abstract
Objective
This study aims at investigating how AI-based transformers can support researchers in designing and conducting an epidemiological study. To accomplish this, we used ChatGPT to reformulate the STROBE recommendations into a list of questions to be answered by the transformer itself. We then qualitatively evaluated the coherence and relevance of the transformer’s outputs.

Study design
Descriptive study.

Methods
We first chose a study to be used as a basis for the simulation. We then used ChatGPT to transform each STROBE checklist’s item into specific prompts. Each answer to the respective prompt was evaluated by independent researchers in terms of coherence and relevance.

Results
The mean scores assigned to each prompt were heterogeneous. On average, for the coherence domain, the overall mean score was 3.6 out of 5.0, and for relevance it was 3.3 out of 5.0. The lowest scores were assigned to items belonging to the Methods section of the checklist.

Conclusions
ChatGPT can be considered as a valuable support for researchers in conducting an epidemiological study, following internationally recognized guidelines and standards. It is crucial for the users to have knowledge on the subject and a critical mindset when evaluating the outputs. The potential benefits of AI in scientific research and publishing are undeniable, but it is crucial to address the risks, and the ethical and legal consequences associated with its use.
Ductile tearing of aluminium plates: experiments and modelling
Authors: Espeseth Vetle , Morin David , Tekoğlu Cihan , Børvik Tore , Hopperstad Odd Sture 
Published: May 2023

AbstractThis paper presents an experimental and numerical study on quasi-static ductile tearing of thin plates of the aluminium alloy AA6016 in three tempers. Depending on the temper, the main fracture mechanism in the plate tearing tests changes from grain boundary failure to coalescence of voids nucleated at the constituent particles. The experiments are complemented by nonlinear finite element simulations using an enriched Gurson–Tvergaard–Needleman (GTN) model to describe the material response. The onset of accelerated void growth is initiated either by incipient material softening (named the softening model) or by the occurrence of strain localization (named the localization model). It was found that strain localization takes place at a critical porosity $$f_\text{ c }$$

f

c

, which depends on the current hydrostatic and deviatoric stress states. While the failure strain depends on the stress path, the critical porosity appears to be path independent. A third method is proposed (named the $$f_\text{ c }(T,L)$$

f

c

(
T
,
L
)

model), where a critical porosity surface $$f_\text{ c } = f_\text{ c }\left( T,L\right) $$

f

c

=

f

c

T
,
L

is used to determine when accelerated void growth starts. The surface is generated beforehand by solving for strain localization under proportional stress states defined by the stress triaxiality T and the Lode parameter L. By comparing the simulations to the experiments, it was found that the localization model performed well for a wide range of stress states. The softening model does not portray dependence on the Lode parameter and is therefore less versatile. The localization model and the $$f_\text{ c }(T,L)$$

f

c

(
T
,
L
)

model gave similar predictions, but some minor differences were observed for two of the three tempers.
“Impact of aging on maximal oxygen uptake adjusted for lower limb lean mass, total body mass, and absolute values in runners”
Authors: Seffrin Aldo , Vivan Lavínia , dos Anjos Souza Vinícius Ribeiro , da Cunha Ronaldo Alves , de Lira Claudio Andre Barbosa , Vancini Rodrigo Luiz , Weiss Katja , Knechtle Beat , Andrade Marilia Santos 
Published: May 2023

Abstract

Performance in endurance sports decreases with aging, which has been primarily attributed to cardiovascular and musculoskeletal aging; however, there is still no clear information on the factors that are most affected by aging. The aim of this study was to compare two groups of runners (< 50 and > 50 years of age) according to their absolute, weight-adjusted maximal oxygen uptake (V̇O2max), lower limb lean mass-adjusted V̇O2max, ventilatory threshold, and respiratory compensation point (RCP). A total of 78 male recreational long-distance runners were divided into Group 1 (38.12 ± 6.87 years) and Group 2 (57.55 ± 6.14 years). Participants were evaluated for body composition, V̇O2max, VT, and RCP. Group 1 showed higher absolute and body mass-adjusted V̇O2max (4.60 ± 0.57 l·min−1 and 61.95 ± 8.25 ml·kg−1·min−1, respectively) than Group 2 (3.77 ± 0.56 l·min−1 and 51.50 ± 10.22 ml·kg−1·min−1, respectively), indicating a significant difference (p < 0.001, d =  − 1.46 and p < 0.001, d =  − 1.16). Correspondingly, Group 1 showed a significantly higher lower limb lean mass–adjusted V̇O2max (251.72 ± 29.60 ml·kgLM−1·min−1) than Group 2 (226.36 ± 43.94 ml·kgLM−1·min−1) (p = 0.008, d =  − 0.71). VT (%V̇O2max) (p = 0.19, d = 0.19) and RCP (%V̇O2max) (p = 0.24, d = 0.22) did not differ between the groups. These findings suggest that both variables that are limited by central or peripheral conditions are negatively affected by aging, but the magnitude of the effect is higher in variables limited by central conditions. These results contribute to our understanding of how aging affects master runners.
Extending bioelectric navigation for displacement and direction detection
Authors: Maier Heiko , Schunkert Heribert , Navab Nassir 
Published: May 2023

Abstract
Purpose
Bioelectric navigation is a navigation modality for minimally invasive endovascular procedures promising non-fluoroscopic navigation. However, the method offers only limited navigation accuracy between anatomical features and expects the tracked catheter to move only in one direction at all times. We propose to extend bioelectric navigation with additional sensing capabilities, allowing for the estimation of the distance traveled by the catheter, thereby improving accuracy between feature locations and allowing to track also under alternating forward- and backward motion.

Methods
We perform experiments in finite element method (FEM) simulations and in a 3D printed phantom. A solution for estimating the traveled distance using a stationary electrode is proposed, together with an approach on how to evaluate the signals obtained with this additional electrode. We investigate the effects of surrounding tissue conductance on this approach. Finally, the approach is refined in order to mitigate the effects of parallel conductance on the navigation accuracy.

Results
The approach allows to estimate the catheter movement direction and the distance traveled. Simulations show absolute errors below 0.89 mm for non-conducting surrounding tissue, but errors up to 60.27 mm when the tissue is electrically conductive. This effect can be mitigated by a more sophisticated modeling (errors up to 33.96 mm). In experiments in a 3D printed phantom, the mean absolute error over 6 catheter paths is 6.3 mm, with standard deviations smaller than or equal to 1.1 mm.

Conclusions
Extending the setup of bioelectric navigation with an additional stationary electrode allows to estimate the distance traveled by the catheter, as well as the movement direction. The effects of parallel conductive tissue could be partially mitigated in simulations, but further research is needed to investigate these effects in real biological tissue, and to bring the introduced errors down to a clinically acceptable level.
Development of a Chief Resident Medical Procedure Service: a 10-Year Experience
Authors: Nathanson Robert , Baher Hasan , Phillips Jason , Freeman Megan , Sehgal Raj , O’Rorke Jane , Soni Nilam J. 
Published: May 2023

Abstract
Background
Lack of experienced faculty to supervise internal medicine (IM) residents is a significant barrier to establishing a medical procedure service (MPS).

Aim
Describe the development and 10-year outcomes of an MPS led by IM chief residents.

Setting
University-based IM residency program affiliated with a county and Veterans Affairs hospital.

Participants
Categorical IM interns (n=320) and 4th-year IM chief residents (n=48) from 2011 to 2022.

Program Description
The MPS operated on weekdays, 8 am–5 pm. After training and sign-off by the MPS director, chief residents trained and supervised interns in ultrasound-guided procedures during a 4-week rotation.

Program Evaluation
From 2011 to 2022, our MPS received 5967 consults and 4465 (75%) procedures were attempted. Overall procedure success, complication, and major complication rates were 94%, 2.6%, and 0.6%, respectively. Success and complication rates for paracentesis (n=2285) were 99% and 1.1%, respectively; 99% and 4.2% for thoracentesis (n=1167); 76% and 4.5% for lumbar puncture (n=883); 83% and 1.2% for knee arthrocentesis (n=85); and 76% and 0% for central venous catheterization (n=45). The rotation was rated 4.6 out of 5 for overall learning quality.

Discussion
A chief resident–led MPS is a practical and safe approach for IM residency programs to establish an MPS when experienced attending physicians are unavailable.