bluuebunny/crossref_metadata_2025_split huggingface dataset:
#5414 CLINICAL UTILITY OF SEROLOGICAL TEST AT TIME OF BIOPSY
Authors: Segelmark Mårten , Weiner Maria , Peters Björn 
Published: June 2023

Abstract

Background and Aims
It is common practice to order serological tests at time of renal biopsy, but the diagnostic value in different clinical settings is largely unknown. We utilized data from the national Swedish Renal Biopsy Registry to analyze the positive and negative post-test likelihood of four common diagnoses depending on the results of frequently used serological tests.

Method
Data on all biopsies performed during the period 2015-01-01 – 2020-12-31 was retrieved from the Swedish Renal Biopsy Registry. All biopsies are classified into one of the following five biopsy indications: the nephrotic syndrome (nephrosis), acute/subacute nephritic syndrome (nephritis), other acute kidney injury (AKI), chronic kidney disease stage 1-2 (CKD 1-2), and CKD 3-5. Biopsy results are given as SNOMED codes by the pathologist and a clinical diagnosis using the ERA coding system by the nephrologist. Results from the following serological tests were analyzed: C3, C4, ANA, anti-dsDNA, ANCA (PR3 and/or MPO-ANCA) and anti-phospholipid antibodies. Pre-test likelihood, positive post-test (PPL) and negative post-test likelihood (NPL) for the diagnoses IgA nephropathy (IgAN), diabetic nephropathy (DN), ANCA-associated vasculitis (AAV) and lupus nephritis (LN) were analyzed. The diagnostic yield was defined as the ratio between the PPL and NPL.

Results
Out of a total of 3165 individuals that had undergone kidney biopsy, there were 694 (21.9%) patients assigned a diagnosis of IgAN, 378 (11.9%) with DN, 342 (10.8%) with AAV and 117 (3.7%) with LN. The proportion of patients being tested varied from 17% for anti-phospholipid antibodies to 77% for ANCA. The highest diagnostic yield was found for ANCA in AAV were the PPL/NPL ratio was 154. In patients with nephrosis the ratio went up to 290 mainly because of a very low NPL (0.2%). The next best diagnostic yield was seen in SLE were both ANA and anti-dsDNA exhibited a PPL/NPL ratio of 26. The best yield for ANA was found when the biopsy indication was nephrosis where the PPL was 33% and the NPL 0.54% and the ratio 61. For anti-dsDNA the best yield was in AKI with a ratio of 46 (PPL 55%, NPL 1.1%). The best test to make a diagnosis less likely was anti-phospholipid antibodies in diabetic nephropathy. The test was never positive in any of the 42 patients with this diagnosis, however the same result slightly increased the likelihood of AAV. A positive result for C3(reduced levels) also reduced the likelihood av diabetic nephropathy, the PPL / NPL ratio was 13. C4 and ANCA being, on the other hand, reduced the likelihood for IgAN with a ratio in the same range.

Conclusion
While the utility of ANCA, ANA and anti-dsDNA is well known, it is less known when a positive test substantially reduced the likelihood of a disease. Here we present data showing that a positive test for C3 decreases the likelihood of DN, while the likelihood is much less affected by C4. The opposite Is seen for IgAN.
#5475 SAFETY OF NATIVE AND TRANSPLANT KIDNEY BIOPSY IN A NATIONAL COHORT
Authors: Mcquarrie Emily , Bell Samira , Campbell Jacqueline , Watters Chrissie , Lakey Joe , Metcalfe Wendy , Hunter Robert , Stevens Kate , Traynor Jamie , Cousland Zoe , Joss Nicola , Walbaum David , Kipgen David , Crosby Jana , Kelly Michael , Dey Vishal , Buck Kate , Methven Shona , Stewart Graham , Murugan Kiru , Conway Bryan , Geddes Colin C 
Published: June 2023

Abstract

Background and Aims
Safety of kidney biopsy is variably reported in different published series. Since 2014, all native kidney biopsies undertaken in the 9 adult renal units in Scotland have been recorded by the Scottish Renal Registry (SRR) and since 2015 all transplant kidney biopsies were included. In this complete national dataset, we report data on safety of kidney biopsy in a current real world setting.

Method
Major complications of kidney biopsy are recorded using pre-defined terms and include: arteriography and embolisation, arteriography no embolisation, clot retention, blood transfusion only, death within 28 days directly attributable to biopsy, nephrectomy and other. Biopsies are undertaken under ultrasound guidance using 16G or 18G spring loaded biopsy guns. All centres discontinue clopidogrel, DOACs and warfarin. Some centres continue aspirin. In some centres biopsy is performed by nephrologists and in others by radiologists

Results
In total, 6979 biopsies in 5755 patients were recorded between 2014 and 2021 (5095 native biopsies and 1884 transplant biopsies), with an adequacy for diagnosis of 98.1%. Table 1 describes the demographics, indications, operator and diagnoses made by biopsy type. Overall, in patients undergoing native kidney biopsy 2.4% suffered a major complication and 1.4% of patients undergoing transplant biopsy. The commonest complication was the requirement for arteriography, with or without embolisation. We included CT angiography in this group. There were 8 deaths within 28 days attributable to renal biopsy.

Conclusion
Kidney biopsy remains safe for the vast majority of patients and complications are less likely with transplant biopsy.
#5946 MULTICENTER STUDY EVALUATING THE FREQUENCY OF ADVERSE EVENTS ASSOCIATED WITH THE SARS-COV2 VACCINATION AMONG PATIENTS WITH GLOMERULAR DISEASES
Authors: Lionaki Sophia , Kriki Pelagia , Marinaki Smaragdi , Dounousi Evaggelia , Flouda Sophia , Fanouriakis Antonios , Liakopoulos Vasileios , Vaios Vasileios , Sardeli Angeliki , Kleinaki Zoe , Petrou Dimitra , Agoranou Marilena , Gkika-Zervou Louisa , Goumenos Dimitrios , Papasotiriou Marios , Venetsanopoulou Aliki , Voulgari Paraskevi , Grapsa Eirini , Andronikidi E , Stylianou Konstantinos , Panagoutsos Stylianos , Boletis Ioannis 
Published: June 2023

Abstract

Background and Aims
Development of SARS-CoV-2 vaccination has altered the natural course of the related infection and the pandemic. The present study aims to explore the frequency of adverse events in patients with glomerular diseases (GD).

Methods
Patients with biopsy-broven GD, who received at least one dose of the vaccine against SARS-CoV-2 were studied retrospectively. Patients who ended up in ESKD prior to vaccination were excluded. We recorded demographics, histopathological diagnosis, past medical history, immunosuppressive regimens which were given at diagnosis and thereafter, outcome of the GD and adverse events associated with the vaccine, both systemic and local. We also estimated the rate of GD relapse post vaccination in patients who were in remission of the primary diseases compared with patients who decided not to receive the vaccine.

Results
To date 280 patients with GD have been included in the study with a mean age of 47,6 (±17,8) years, of whom 111 (39,6%) are males. Patients received in total 3,0 (±0,9) vaccine doses with the mean time from the diagnostic kidney biopsy to the 1st dose of being 76,5 (±61,5) months. 47,1% of the patients were on immunosuppressive therapy at the time of vaccination. 27,1% of the patients reported systemic side effects (fever, arthralgias, myalgias) and 50,7% reported local side effects (pain, swelling, itching, edema). Renal function and 24-hour proteinuria remained stable after vaccination. Among patients who were in remission of the GD at the time of vaccination 19 (8,2%) patients experienced a relapse of the primary disease after vaccination, versus 5% of patients not vaccinated (p = 0,99).

Conclusions
According to our findings the vaccine against SARS-CoV-2 appears safe for patients with GD with no significant impact in renal function or the probability for relapse.
#4646 CT-DERIVED RADIOMICS ANALYSIS OF DIABETIC NEPHROPATHY BY MACHINE LEARNING MODELS
Authors: Chung Eui Seok , Lee Eun Ji , Yoon Jongjin , Lee Haekyung , Kim Hyoungnae , Noh Hyunjin , Kwon Soon Hyo , Jeon Jin Seok 
Published: June 2023

Abstract

Background and Aims
Kidney radiomics has been used to develop more accurate diagnostic tools of renal tumor and predict outcomes. However, radiomics studies for diabetic kidney disease (DKD) remain few. In this light, we hypothesized that computed tomography (CT) radiomics features could differentiate DKD from normal kidneys and assess the severity of DKD.

Method
This retrospective study included 343 subjects with type 2 diabetes mellitus (T2DM) (male 65.5%, mean age 63.6±14.8) and 90 healthy controls (HC) (male 34.4%, mean age 41.9±8.5) who underwent abdominal CT. Whole volumetric CT data of both kidneys were automatically extracted using a deep-learning based model and radiomics features were extracted. T2DM were categorized into three groups according to eGFR (mL/min/1.73 m^2) (group 1, eGFR &gt; 60; group 2, 15 ≤ eGFR ≤ 60; and group 3, eGFR &lt;15). The capability of CT radiomics features to distinguish not only DKD from HC but also various DKD groups based on eGFR, was evaluated using machine learning models.

Results
A total of 1,723 radiomics features were extracted from the volumetric CT data of both kidneys. A combination of LASSO filter and random forest showed best performance in differentiating between HC and DKD, with an area under curve (AUC) of 0.98 and accuracy of 95.7%. It also showed an excellent performance in differentiating between HC and DKD group 1 with AUC of 0.97 and accuracy of 91.7%, and also it was able to differentiate between DKD groups (AUCs &gt; 0.78). CT radiomics features and eGFR had a moderate degree of correlation (R=-0.50 to R=0.63, all P&lt;.00).

Conclusion
CT-derived radiomics analysis of kidneys using machine learning models can be useful in differentiating DKD from normal kidneys as well as assessing the severity of DKD.
#6794 CALCIUM/MAGNESIUM RATIO IN PATIENTS WITH DIABETES AND CHRONIC KIDNEY DISEASE: A RISK FACTOR FOR CARDIOVASCULAR DISEASE
Authors: Borges Henrique , Afonso Rita , Marques Roberto Calças , Jerónimo Teresa , Silva Ana 
Published: June 2023

Abstract

Background and Aims
Chronic Kidney Disease (CKD) and Diabetes Mellitus (DM) are significant risk factors for Cardiovascular (CV) Disease. Patients with CKD and/or DM exhibit higher incidence and prevalence of CV events compared to the general population. Hypomagnesemia and elevated calcium-magnesium (Ca:Mg) ratios have been identified as independent risk factors for CV-related deaths. The aim of this study is to determine the relationship between Ca:Mg and the prognosis of CV disease in patients with CKD and DM.

Method
This cross-sectional study enrolled patients with DM and CKD followed at Diabetic Nephrology appointments in a hospital. The study population was divided into two groups: Group 1 (G1) - patients who had a previous hospitalization due to a CV event, and Group 2 (G2) - patients without CV-related hospitalizations. A logistic regression model was employed to evaluate the predictive factors for CV hospitalization for the variables studied.

Results
A total of 223 patients were included in the study, 92 females and 131 males, with an estimated glomerular filtration rate of 38.4±22 mL/min/1.73 m2 and 41% had been hospitalized due to a CV event. G1 had higher age (P = .02), higher serum phosphate (p&lt;0.01), parathormone (p&lt;0.01), interleukin-6 (p&lt;0.01), FGF-23 (p&lt;0.01), oxidized-LDL (p&lt;0.01), Ca:Mg (p&lt;0.01), and pulse pressure (p&lt;0.01), as well as lower eGFR (p&lt;0.01) and magnesium serum levels (p&lt;0.01). The multivariate logistic regression model revealed that higher FGF-23 levels (OR=3.44, 95% CI 1.192-9.954, P = .02), Ca:Mg (OR=1.204, 95% CI 2.072-59.040, P = .01), oxidized LDL (OR=9.746, 95% CI 3.277-29.020, p&lt;0.01), and an eGFR &lt;30 mL/min/1.73 m2 (OR=8.685, 95% CI 1.277-59.040, P = .03) were predictors of CV morbidity.

Conclusion
In this population, Ca:Mg and FGF-23 were predictors of CV morbidity. Further studies are necessary to fully understand the relationship between serum magnesium and calcium levels and CV events. Monitoring these parameters may be valuable in clinical practice to identify high-risk patients.
Determinants of cervical spine disorders in military pilots: a systematic review
Authors: Al Rumaithi M , Al Qubaisi M , Al Suwaidi M , Al Zaabi F , Campos L A , Baltatu O C , Al Tunaiji H 
Published: June 2023

Abstract

Background
Neck pain and cervical spine disorders are widespread among military cockpit aircrew pilots and are often recognized as occupational stressors.

Aims
This systematic review aimed to identify significant determinants for military pilot neck pain and cervical spine disorders determined through multivariable logistic regression studies.

Methods
This systematic review was conducted according to the recommendations of the Statement of Systematic Review and Meta-analysis Protocols (Preferred Reporting Items for Systematic Reviews and Meta-Analyses [PRISMA]-P). The following databases were searched for literature: Medline and Embase. We included studies that studied neck pain, cervical spine disorders, and/or radiological abnormalities and associated exposures (adjusted odds ratios, ORadj) in military cockpit aircrew. The trustworthiness, relevance and results of the published papers were evaluated using the Joanna Briggs Institute critical checklist.

Results
A total of three studies quantified the strength of the correlations between exposures and outcomes. Significant determinants/risk factors of neck pain, cervical spine disorders and radiological abnormalities were identified as age (ORadj: 1.092 [95% CI 1.054, 1.132]), fighter type (ORadj: 3.9 [95% CI 1.1, 13.9]) and absolute rotation angle of C2-7 (ARA) (ORadj: 0.91 [CI 0.85, 0.98]). The following variables were unable to demonstrate statistical significance: flying hours, body height and body mass index.

Conclusions
Military cockpit aircrew’s frequent neck pain after a flight raises concerns about cervical spine disorders. Age, fighter type and ARA C2-7 are strong predictors of neck pain and cervical spine disorders. More research is needed on occupational determinants and risk factors for neck pain and cervical spine disorders in military cockpit aircrew.
Genetic factors for differentiated thyroid cancer in French Polynesia: new candidate loci
Authors: Zidane Monia , Haber Marc , Truong Thérèse , Rachédi Frédérique , Ory Catherine , Chevillard Sylvie , Blanché Hélène , Olaso Robert , Boland Anne , Conte Éric , Karimi Mojgan , Ren Yan , Xhaard Constance , Souchard Vincent , Gardon Jacques , Taquet Marc , Bouville André , Deleuze Jean-François , Drozdovitch Vladimir , de Vathaire Florent , Cazier Jean-Baptiste 
Published: June 2023

Abstract

Background
Populations of French Polynesia (FP), where France performed atmospheric tests between 1966 and 1974, experience a high incidence of differentiated thyroid cancer (DTC). However, up to now, no sufficiently large study of DTC genetic factors in this population has been performed to reach definitive conclusion. This research aimed to analyze the genetic factors of DTC risk among the native FP populations.

Methods
We analyzed more than 300 000 single nucleotide polymorphisms (SNPs) genotyped in 283 DTC cases and 418 matched controls born in FP, most being younger than 15 years old at the time of the first nuclear tests. We analyzed the genetic profile of our cohort to identify population subgroups. We then completed a genome-wide analysis study on the whole population.

Results
We identified a specific genetic structure in the FP population reflecting admixture from Asian and European populations. We identified three regions associated with increased DTC risk at 6q24.3, 10p12.2, and 17q21.32. The lead SNPs at these loci showed respective p-values of 1.66 × 10−7, 2.39 × 10−7, and 7.19 × 10−7 and corresponding odds ratios of 2.02, 1.89, and 2.37.

Conclusion
Our study results suggest a role of the loci 6q24.3, 10p12.2 and 17q21.32 in DTC risk. However, a whole genome sequencing approach would be better suited to characterize these factors than genotyping with microarray chip designed for the Caucasian population. Moreover, the functional impact of these three new loci needs to be further explored and validated.
Temporal and vertical dynamics of diatoms and dinoflagellates in the southeastern Mediterranean Sea
Authors: Rahav Eyal , Berman-Frank Ilana 
Published: June 2023

AbstractThe temporal distributions of microphytoplankton were studied monthly over 2 years in the shelf and the offshore waters of the “low nutrients low chlorophyll” (LNLC) southeastern (SE) Mediterranean Sea. The microphytoplankton were comprised mostly of diatoms (0–542 cells L−1, ~4 × 106–44 × 106 cells m−2) of the genera Rhizosolenia, Thalassionema, Chaetoceros, Leptocylindrus and Pseudo-nitzschia, and dinoflagellates (1–89 cells L−1, ~1 × 106–10 × 106 cells m−2) of the genera Tripos (formally known as Ceratium), Ornithocercus, Protoperidinium, Ceratocorys and Dinophysis. During the winter mixing, microphytoplankton abundance was ~2-fold higher than during the stratified summer months, in accordance with the higher inorganic nutrient levels. Diatoms were mostly found in the upper illuminated layers (0–100 m), while dinoflagellate distribution was patchy and spread from surface down to 200 m. We also calculated that diatoms and dinoflagellates contribute negligibly (~4%) to the total vertical carbon flux in the offshore water, suggesting fast recycling of organic matter at the photic layer. Our results provide a baseline to better understand carbon and biogenic silica fluxes at the LNLC SE Mediterranean Sea.
Genome-wide high-throughput chromosome conformation capture analysis reveals hierarchical chromatin interactions during early somatic embryogenesis
Authors: Chen Yan , Xie Dejian , Ma Xiangwei , Xue Xiaodong , Liu Mengyu , Xiao Xuechen , Lai Chunwang , Xu Xiaoping , Chen Xiaohui , Chen Yukun , Zhang Zihao , XuHan Xu , Lai Zhongxiong , Lin Yuling 
Published: June 2023

Abstract
Somatic embryogenesis (SE), like zygotic embryo development, is a progressive process. Early SE is the beginning of a switch from a somatic to an embryogenic state and is an important stage for initiating chromatin reprogramming of SE. Previous studies suggest that changes in chromatin accessibility occur during early SE, although information on the 3D structure of chromatin is not yet available. Here, we present a chromosome-level genome assembly of longan (Dimocarpus longan) using PacBio combined with high-through chromosome conformation capture scaffolding, which resulted in a 446 Mb genome assembly anchored onto 15 scaffolds. During early SE, chromatin was concentrated and then decondensed, and a large number of long terminal repeat retrotransposons (LTR-RTs) were enriched in the local chromatin interaction region, suggesting LTR-RTs were involved in chromatin reorganization. Early SE was accompanied by the transformation from A to B compartments, and the interactions between B compartments were enhanced. Results from chromatin accessibility, monomethylation of histone H3 at lysine 4 (H3K4me1) modification, and transcription analyses further revealed a gene regulatory network for cell wall thickening during SE. Particularly, we found that the H3K4me1 differential peak binding motif showed abnormal activation of ethylene response factor transcription factors and participation in SE. The chromosome-level genomic and multiomics analyses revealed the 3D conformation of chromatin during early SE, providing insight into the molecular mechanisms underlying cell wall thickening and the potential regulatory networks of TFs during early SE in D. longan. These results provide additional clues for revealing the molecular mechanisms of plant SE.
Autophagy in maternal tissues contributes to Arabidopsis seed development
Authors: Erlichman Ori Avraham , Weiss Shahar , Abu Arkia Maria , Ankary-Khaner Moria , Soroka Yoram , Jasinska Weronika , Rosental Leah , Brotman Yariv , Avin-Wittenberg Tamar 
Published: June 2023

Abstract
Seeds are an essential food source, providing nutrients for germination and early seedling growth. Degradation events in the seed and the mother plant accompany seed development, including autophagy, which facilitates cellular component breakdown in the lytic organelle. Autophagy influences various aspects of plant physiology, specifically nutrient availability and remobilization, suggesting its involvement in source–sink interactions. During seed development, autophagy affects nutrient remobilization from mother plants and functions in the embryo. However, it is impossible to distinguish between the contribution of autophagy in the source (i.e. the mother plant) and the sink tissue (i.e. the embryo) when using autophagy knockout (atg mutant) plants. To address this, we employed an approach to differentiate between autophagy in source and sink tissues. We investigated how autophagy in the maternal tissue affects seed development by performing reciprocal crosses between wild type and atg mutant Arabidopsis (Arabidopsis thaliana) plants. Although F1 seedlings possessed a functional autophagy mechanism, etiolated F1 plants from maternal atg mutants displayed reduced growth. This was attributed to altered protein but not lipid accumulation in the seeds, suggesting autophagy differentially regulates carbon and nitrogen remobilization. Surprisingly, F1 seeds of maternal atg mutants exhibited faster germination, resulting from altered seed coat development. Our study emphasizes the importance of examining autophagy in a tissue-specific manner, revealing valuable insights into the interplay between different tissues during seed development. It also sheds light on the tissue-specific functions of autophagy, offering potential for research into the underlying mechanisms governing seed development and crop yield.
The social context of burning mouth syndrome: an exploratory pilot study of stigma, discrimination, and pain
Authors: Mathur Vani A , Payano Sosa Janell S , Keaser Michael L , Meiller Timothy F , Seminowicz David A 
Published: June 2023

Abstract

Background
The social context of burning mouth syndrome (BMS) has received little attention in the scientific literature. However, social psychological theory and insights from those with lived experiences suggest that people living with BMS experience compounding effects of stigma related to their pain, diagnosis (or lack thereof), and intersectional identities.

Objective
Our aim is to provide initial evidence and to motivate new directions for research on BMS. Here, we present the results of an exploratory pilot study (n = 16) of women living with BMS in the United States.

Methods
Participants completed self-report measures of stigma, discrimination, and pain, as well as laboratory assessments of pain through quantitative sensory testing.

Results
Results indicate a high prevalence of internalized BMS stigma, experience of BMS-related discrimination from clinicians, and gender stigma consciousness in this population. Moreover, results provide initial evidence that these experiences are related to pain outcomes. The most robust pattern of findings is that internalized BMS stigma was related to greater clinical pain severity, interference, intensity, and unpleasantness.

Conclusion
Given the prevalence and pain-relevance of intersectional stigma and discrimination identified in this pilot study, lived experience and social context should be incorporated into future research on BMS.