bluuebunny/crossref_metadata_2025_split huggingface dataset:
Human Cytomegalovirus UL138 Interaction with USP1 Activates STAT1 in infection
Authors: Zarrella Kristen , Longmire Pierce , Zeltzer Sebastian , Collins-McMillen Donna , Hancock Meaghan , Buehler Jason , Reitsma Justin M. , Terhune Scott S. , Nelson Jay A. , Goodrum Felicia 
Published: February 2023

AbstractInnate immune responses are crucial for limiting virus infection. However, viruses often hijack our best defenses for viral objectives. Human Cytomegalovirus (HCMV) is a beta herpesvirus which establishes a life-long latent infection. Defining the virus-host interactions controlling latency and reactivation is vital to the control of viral disease risk posed by virus reactivation. We defined an interaction between UL138, a pro-latency HCMV gene, and the host deubiquintase complex, UAF1-USP1. UAF1 is a scaffold protein pivotal for the activity of ubiquitin specific peptidases (USP), including USP1. UAF1-USP1 sustains an innate immune response through the phosphorylation and activation of signal transducer and activator of transcription-1 (pSTAT1), as well as regulates the DNA damage response. After the onset of viral DNA synthesis, pSTAT1 levels are elevated and this depends upon UL138 and USP1. pSTAT1 localizes to viral centers of replication, binds to the viral genome, and influences UL138 expression. Inhibition of USP1 results in a failure to establish latency, marked by increased viral genome replication and production of viral progeny. Inhibition of Jak-STAT signaling also results in increased viral genome synthesis in hematopoietic cells, consistent with a role for USP1-mediated regulation of STAT1 signaling in the establishment of latency. These findings demonstrate the importance of the UL138-UAF1-USP1 virus-host interaction in regulating HCMV latency establishment through the control of innate immune signaling. It will be important going forward to distinguish roles of UAF1-USP1 in regulating pSTAT1 relative to its role in the DNA damage response in HCMV infection.ImportanceHuman cytomegalovirus (HCMV) is one of nine herpesviruses that infect humans. Following a primary infection, HCMV establishes a life-long latent infection that is marked by sporadic, and likely frequent reactivation events. While these reactivation events are asymptomatic in the immune competent host, they pose important disease risks for the immune compromised, including solid organ or stem cell transplant recipients. Its complex interactions with host biology and deep coding capacity make it an excellent model for defining mechanisms important for viral latency and reactivation. Here we define an interaction with host proteins that commandeer typically antiviral innate immune signaling for the establishment of latency.
Delayed exposure of rice to light partially phenocopies C4bundle sheath characteristics by reducing cell length
Authors: Plackett Andrew R.G. , Hibberd Julian M. 
Published: February 2023

ABSTRACTC4photosynthesis has arisen in over sixty lineages of angiosperms. Despite significant variation in characteristics of the C4leaf, in all cases a compartment rich in chloroplasts allows high concentrations of CO2to be supplied to RuBisCO. In most C4lineages this is associated with increased chloroplast content of bundle sheath cells. As characteristics of C4leaves are derived from processes operating in the ancestral C3state, we studied greening of the bundle sheath in C3rice. Total visible chloroplast area in bundle sheath and mesophyll cells was similar but the larger volume of bundle sheath cells reduced the proportion of cell occupied by chloroplast, and the longest bundle sheath cells had the lowest chloroplast content. Application of exogenous cytokinin and gibberellin increased chloroplast content of the longest bundle sheath cells but did not change cell shape. In contrast, delayed exposure to light reduced the number of very long bundle sheath cells but increased leaf length indicating delayed exit from the cell cycle. We conclude that in the C3state, the plant hormones cytokinin and gibberellin are regulators of the bundle sheath cell-chloroplast relationship and that final bundle sheath length is affected by light-mediated control of exit from the cell cycle.HighlightExtended darkness can reduce maximum bundle sheath cell length in rice and hormones condition the set-point between cell size and chloroplast content.
Co-evolution of Large inverted repeats and G-quadruplex DNA in fungal mitochondria may facilitate mitogenome stability: the case ofMalassezia
Authors: Christinaki Anastasia C. , Theelen Bart , Zania Alkmini , Coutinho Selene Dall’ Acqua , Cabañes Javier F. , Boekhout Teun , Kouvelis Vassili N. 
Published: February 2023

AbstractMitogenomes are essential due to their contribution to cell respiration. Recently they have also been implicated in fungal pathogenicity mechanisms. Members of the basidiomycetous yeast genusMalasseziaare an important fungal component of the human skin microbiome, linked to various skin diseases, bloodstream infections, and increasingly implicated in gut diseases and certain cancers. In this study, the comparative analysis ofMalasseziamitogenomes contributed to phylogenetic tree construction for all species. The mitogenomes presented significant size and gene order diversity which correlates to their phylogeny. Most importantly, they showed the inclusion of Large Inverted Repeats (LIRs) and G-quadruplex (G4) DNA elements, renderingMalasseziamitogenomes a valuable test case for elucidating the evolutionary mechanisms responsible for this genome diversity. Both LIRs and G4s coexist and convergently evolved to provide genome stability through recombination. This mechanism is common in chloroplasts but, hitherto, rarely found in mitogenomes.
The effects of perceived value dimensions on customer satisfaction and loyalty: a comparison between traditional banks and fintechs
Authors: Mainardes Emerson Wagner , Freitas Neudson Peres de 
Published: February 2023

PurposeThis study aims to verify the influence of perceived value dimensions on customer satisfaction and loyalty in the banking sector, comparing these relationships between traditional banks and fintechs. Also, it was verified whether satisfaction mediates the relationships between the dimensions of perceived value and customer loyalty to traditional banks and fintechs, comparing them.Design/methodology/approachData were collected through two online questionnaires with 792 total respondents, 411 from traditional banks and 381 from fintechs. For data analysis, the authors used the Partial Least Squares - Structural Equation Modeling (PLS-SEM) and PLS-SEM multigroup analysis (PLS-MGA).FindingsThe influence of customer satisfaction on loyalty tends to be greater in traditional banks than in fintechs; the effect of reliability on satisfaction tends to be greater in fintechs than in traditional banks and the effect of price on satisfaction tends to be greater in traditional banks than in fintechs. Indirectly, empathy, price and competence influence loyalty through satisfaction, and in all these relationships, the strength of the effect is significantly greater in traditional banks when compared to fintechs.Research limitations/implicationsThe findings, on the one hand, indicate that banks' investments in customer satisfaction, empathy, price and competence tend to generate positive results by expanding customer loyalty in addition to the return on similar investments made by fintechs. On the other hand, when fintechs invest in reliability, they tend to capture better results in increasing customer satisfaction compared to traditional banks.Originality/valueThe comparison of the effect of the dimensions of perceived value on satisfaction and loyalty between traditional banks and fintechs stands out, which is a novelty in the literature. This comparison can support strategies that aim to strengthen relationships with customers and increase the recurrence of business, both for traditional banks and fintechs.
Advertising as discursive reflections of the political realm: Turkish-Cypriot advertisements between 1940-1974
Authors: Yalkin Cagri , Kahveci Hayriye , Uygur Kubra 
Published: February 2023

Purpose
The purpose of this paper is to explore how conflict/war and its political economic and socio-cultural reflections influenced Turkish-Cypriot advertisements. It provides an analysis of the Turkish-Cypriot advertisements during 1940–1974, which was characterised by intermittent inter-ethnic armed conflict, to illuminate how they are related to the commercial, political, economic and socio-cultural unravelling of the era.

Design/methodology/approach
Compositional interpretation and social semiotic analysis (Rose, 2016) with a critical lens have been adopted as research design. Social, cultural, economic and political conditions of the time were also used in the analysis.

Findings
Firstly, this paper shows that the advertisements increased in complexity although very incrementally through the studied period, especially in comparison to the advertising of the countries that directly influenced the commercial sphere in Cyprus such as Britain, Greece and Turkey. Secondly, this paper shows that the advertisement messages were directly shaped by key events in the socio-political realm. Especially during the 1940s (British rule) and the bicommunal Republic of Cyprus era, the Turkish-Cypriot community newspapers featured a cosmopolitan range of advertisements paid for by Greek-Cypriot, Armenian-Cypriot and Turkish-Cypriot businesses. After 1963, when the armed conflict began and the communities retreated to separate parts of the capital city, the featured advertisements mostly reverted back to the 1950s political economic agenda: firstly, they intended to build ethno-community consciousness by transforming Turkish-Cypriots who were scattered around in different towns, villages and cities into a public/community; secondly, they were used in developing the burgeoning Turkish-Cypriot business enterprises and the making of a consumer class.

Originality/value
As no such study was conducted so far, this paper shows, through the continued and increasing existence and variety of the advertisements printed in Turkish-Cypriot newspapers, the trajectory with which the Turkish-Cypriots met the consumption codes of the era under armed conflict. Secondly, the authors observe the “state” authority (in this case, the community administration) directly and indirectly influences both the business agenda and the building of community consciousness. Thirdly, the authors show that the Turkish-Cypriot community had normalised commerce under armed-conflict.
Stratification and recovery time jointly shape ant functional reassembly in a neotropical forest
Authors: Hoenle Philipp O. , Staab Michael , Donoso David A. , Argoti Adriana , Blüthgen Nico 
Published: February 2023

Abstract

Microhabitat differentiation of species communities such as vertical stratification in tropical forests contributes to species coexistence and thus biodiversity. However, little is known about how the extent of stratification changes during forest recovery and influences community reassembly. Environmental filtering determines community reassembly in time (succession) and in space (stratification), hence functional and phylogenetic composition of species communities are highly dynamic. It is poorly understood if and how these two concurrent filters—forest recovery and stratification—interact.

In a tropical forest chronosequence in Ecuador spanning 34 years of natural recovery, we investigated the recovery trajectory of ant communities in three overlapping strata (ground, leaf litter, lower tree trunk) by quantifying 13 traits, as well as the functional and phylogenetic diversity of the ants. We expected that functional and phylogenetic diversity would increase with recovery time and that each ant community within each stratum would show a distinct functional reassembly. We predicted that traits related to ant diet would show divergent trajectories reflecting an increase in niche differentiation with recovery time. On the other hand, traits related to the abiotic environment were predicted to show convergent trajectories due to a more similar microclimate across strata with increasing recovery age.

Most of the functional traits and the phylogenetic diversity of the ants were clearly stratified, confirming previous findings. However, neither functional nor phylogenetic diversity increased with recovery time. Community‐weighted trait means had complex relationships to recovery time and the majority were shaped by a statistical interaction between recovery time and stratum, confirming our expectations. However, most trait trajectories converged among strata with increasing recovery time regardless of whether they were related to ant diet or environmental conditions.

We confirm the hypothesized interaction among environmental filters during the functional reassembly in tropical forests. Communities in individual strata respond differently to recovery, and possible filter mechanisms likely arise from both abiotic (e.g. microclimate) and biotic (e.g. diet) conditions. Since vertical stratification is prevalent across animal and plant taxa, our results highlight the importance of stratum‐specific analysis in dynamic ecosystems and may generalize beyond ants.
Home gardens, household nutrition and income in rural farm households in Odisha, India
Authors: Ogutu Sylvester O. , Mockshell Jonathan , Garrett James , Labarta Ricardo , Ritter Thea , Martey Edward , Swamikannu Nedumaran , Gotor Elisabetta , Gonzalez Carolina 
Published: February 2023

AbstractHome gardens have been an integral part of the recent food‐based interventions aimed at stimulating changes in dietary patterns and improving nutrition. However, evidence of their effects on food security, dietary quality, child anthropometry and incomes is limited, particularly among vulnerable populations groups. Using panel data from a sample of approximately 1900 households from vulnerable population groups in Odisha, India, difference‐in‐differences and other econometric techniques, we analyse the effects of home gardens on food security, dietary quality, child anthropometry and income. On average, home gardens contribute to better household food security, higher dietary quality of men and women but do not contribute to higher children's dietary quality and anthropometry. Also, home gardens increase monthly per adult equivalent incomes by 37% and reduce the prevalence of poverty by 11.7 percentage points. Quantile regression results suggest that home gardens enhance food security and incomes in all quantiles, but richer farmers benefit more than poorer farmers. Overall, home gardens can enhance household food security, dietary quality of men and women, and income gains among vulnerable farming population groups, but they may not suffice to improve child dietary quality and anthropometry.
Iris metastasis as resistance mechanism to atezolizumab, carboplatin, and etoposide but responds to additional irinotecan and anlotinib in a small cell lung cancer patient
Authors: Huang Zhe , Zhang Yongchang 
Published: February 2023

AbstractSmall cell lung cancer (SCLC) is an aggressive malignancy associated with poor prognosis. Metastasis to sites outside the chest at the time of initial diagnosis, such as bone, brain, and liver metastasis have been found in most SCLC patients. Iris metastases from SCLC have rarely been previously reported, and often cause eye pain and blindness in patients. Here, we report a patient with SCLC who presented with iris metastasis in the right eye and metastasis in the left adrenal gland due to disease progression on first‐line therapy, which subsequently caused pain and blindness in the right eye. The patient was treated with second‐line irinotecan combined with anlotinib and atezolizumab and did not receive any local treatment in the right eye. After only one cycle of treatment, the iris metastases in the right eye were smaller than before, and the visual acuity in the right eye recovered. At the same time, her left adrenal metastases were also significantly smaller than before. Our case suggests that systemic therapy with effective treatment options can similarly improve iris metastases in patients.
Vital function of DRD4 in dapoxetine medicated premature ejaculation treatment
Authors: Gao Pan , Liu Xi , Zhu Tianle , Gao Rui , Gao Jingjing , Zhang Yao , Jiang Hui , Huang Houbao , Zhang Xiansheng 
Published: February 2023

AbstractBackgroundRecently, dapoxetine has been widely accepted to treat premature ejaculation by fast‐inhibiting 5‐hydroxytryptamine reuptake. However, dapoxetine is not suitable for all premature ejaculation patients in clinical treatment. We need to investigate and reveal the mechanism deeply to solve this problem.ObjectivesTo investigate and reveal the function of dopamine D4 receptor in dapoxetine medicated premature ejaculation treatment.Materials and methodsA rat model was used to screen rapid ejaculators. The molecular mechanisms of histone serotonylation‐mediated regulation of dopamine D4 receptor were demonstrated by chromatin immunoprecipitation, DNA pull‐down, mass spectrometry analysis, and coimmunoprecipitation experiments. The biological function of dopamine D4 receptor was investigated through in vivo experiments by intrathecal injection of shDRD4 to knockdown dopamine D4 receptor.ResultsIn this study, we found that dapoxetine increased expression of 5‐hydroxytryptamine and dopamine D4 receptor. We demonstrated that dapoxetine increased levels of 5‐hydroxytryptamine, which promoted histone serotonylation (H3K4me3Q5ser) and transcription factor myeloid zinc‐finger 1 complex binding on the dopamine D4 receptor promoter, upregulated the expression of dopamine D4 receptor and thus delayed ejaculation.DiscussionIn this study, we demonstrated that dapoxetine increased the levels of 5‐hydroxytryptamine, which promoted histone serotonylation and myeloid zinc‐finger 1 complex binding to the dopamine D4 receptor promoter and upregulated the expression of dopamine D4 receptor, thus delaying ejaculation.ConclusionIt is a novel mechanism that dapoxetine take effect of premature ejaculation treatment through upregulating the dopamine D4 receptor, which indicated that upregulated dopamine D4 receptor would enhance the dapoxetine effect in premature ejaculation treatment. This may lead to the development of novel therapeutic interventions for premature ejaculation.
How to use mixed methods in oral health research
Authors: Nicolau Belinda , Eslamiamirabadi Negin , Dudubo Olawale , Hong Quan Nha , Bedos Christophe , Macdonald Mary Ellen 
Published: February 2023

AbstractWicked problems exist in the realm of oral health research. Due to their inherent complexity, using qualitative or quantitative methods alone may not be adequate for resolving them. Mixed methods approaches combine qualitative and quantitative methods, and thus, can provide a powerful tool for understanding and solving complex problems in dental public health. However, using mixed methods does not come without its challenges. This commentary outlines four main tips for researchers to consider when applying mixed methods to their research projects.
Effects of carnitine on glucose and lipid metabolic profiles and fertility outcomes in women with polycystic ovary syndrome: A systematic review and meta‐analysis
Authors: Gong Yi , Jiang Tong , He Hui , Wang Yu , Wu Guo‐Lin , Shi Ying , Cai Qinjun , Xiong Can‐Li , Shen Rong , Li Jian 
Published: February 2023

AbstractObjectivesTo quantify the effect of carnitine on glucose and lipid metabolic profiles and fertility outcomes in women with Polycystic ovary syndrome (PCOS).DesignA systematic review and meta‐analysis were conducted.PatientsWomen with PCOS diagnosed by Rotterdam or Androgen Excess Society (AES) criteria and taking carnitine supplement were assessment.MeasurementsFertility outcomes (ovulation, clinical pregnancy, live birth, and miscarriage), lipid parameters (BMI, triglyceride, total cholesterol, high‐density lipoprotein, low‐density lipoprotein), fasting glucose and insulin, and Homeostatic Model Assessment for Insulin Resistance (HOMA‐IR).ResultsIn total, 839 participants were included in this analysis. The dosage of carnitine and treatment duration reported by studies varied from 250 mg to 3000 mg daily and 84 to 90 days, respectively. The publication bias was absent. Compared with placebo, carnitine significantly improved ovulation rates (RR 3.42, 95% CI 2.39 to 4.89, I2 = 0%) and pregnancy rates (RR 11.05, 95% CI 1.21 to 100.58, I2 = 79%). None of included studies reported live birth. After treatment, carnitine resulted in significant reductions relative to baseline in body mass index (BMI, MD −0.93 kg/m2, 95% CI −1.15 to −0.70, I2 = 55.0%), insulin levels (MD −2.47 mIU/L, 95% CI −4.49 to −0.45, I2 = 0%) and the Homeostasis Model Assessment index (MD −0.67, 95% CI −1.20 to −0.14, I2 = 0%) than placebo, but not for lipid profiles including triglyceride, total cholesterol, and low‐density lipoprotein.ConclusionWith the available literature, carnitine seems to improve ovulation and clinical pregnancy and insulin resistance, BMI in women with PCOS. These effects are warranted to be further validated, due to insufficient statistical power.
Clinical significance of papillary thyroid carcinoma with solid/trabecular growth
Authors: Pinheiro Sara Lomelino , Miranda Afonso Pedro , Damásio Inês Lemos , Simões‐Pereira Joana , Nunes da Silva Tiago , Leite Valeriano 
Published: February 2023

AbstractObjectiveThe clinical relevance of solid/trabecular (ST) growth in papillary thyroid carcinoma (PTC) is unclear. In this study, we investigated the impact of any amount of ST growth on tumour characteristics and patient outcomes. Furthermore, we evaluated whether ST growth per se affected patients' prognosis in the absence of aggressive features, namely vascular invasion.DesignWe analysed 222 PTC patients followed up for more than 5 years in the Department of Endocrinology of the Instituto Português de Oncologia de Lisboa Francisco Gentil from 2002 to 2020. All PTC cases with any percentage of ST growth were included and compared with PTC without ST growth (1:2). Carcinomas with high‐grade features were excluded.ResultsThere were 74 PTC cases with ST growth and 148 without ST growth (median follow‐up of 9.3 years). PTC‐ST was associated with larger tumour size (p = 0.001) and increased frequency of vascular invasion (p < 0.001) compared with PTC. However, PTC‐ST did not exhibit a higher incidence of extrathyroidal extension (p = 1.000) or lymph node metastasis (p = 0.433). Despite the significantly higher prevalence of distant metastasis in PTC‐ST compared with PTC (p = 0.043), the significance is lost when the cases with vascular invasion were excluded (p = 0.347). The total radioiodine activity was higher in PTC‐ST than in PTC (p = 0.008). Recurrence rates were similar between groups (p = 0.755). The 10‐year overall survival and disease‐free survival rates for PTC‐ST were 94.6% and 98.6%, respectively, similar to the PCT without ST growth (p = 0.097 and p = 0.333, respectively). There was no evidence of an association between the presence of an ST component (p = 0.201) with the risk of death or recurrence, whereas the presence of distant metastasis significantly increased the risk of these events (hazard ratio 10.14, p < 0.001).ConclusionsThe presence of ST growth was associated with several aggressive clinicopathological features. However, the risk of cancer recurrence and death for PTC‐ST were similar to PTC. In the absence of vascular invasion, the clinical impact of ST growth alone is negligible.
Cover Image
Authors: Shen Xiaoting , Yang Sijia , Xu Yangbo , Qi Wenting , He Fuming 
Published: February 2023

The cover image is based on the Original Article Marginal bone loss of tissue‐ or bone‐level implants after simultaneous guided bone regeneration in the posterior mandibular region: A retrospective cohort study by Xiaoting Shen DDS et al., https://doi.org/10.1111/cid.13144.
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