bluuebunny/crossref_metadata_2025_split huggingface dataset:
Assessment of hearing performance of dental technicians due to the professional noise exposure
Authors: Vaddamanu Sunil Kumar , Alhamoudi Fahad Hussain , Binduhayyim Rayan Ibrahim H. , AlJameel AlBandary Hassan , Alwadi Maram Ali M. , Di Blasio Marco , Cicciù Marco , Minervini Giuseppe 
Published: September 2023

Abstract
Background
Some of the noise-intensive processes in dental laboratories include the finishing of crowns, bridges, and removable partial dentures; blowing out workpieces with steam and compressed air; and deflating casting rings. High sound pressure levels are also present in dental vibrators, polishing equipment, and sandblasters. The aim of this study was to Evaluation of the effect of noise production in dental technology laboratory on dental technician hearing capacity.

Methods
For this cross-sectional study, a total of 120 dental technicians were chosen. Otoscopic evaluation and the Weber test were used to establish if they had sensorineural or transmission hearing loss at 500 Hz, 1000 Hz, 2000 Hz, and 4000 Hz, respectively. Then an OAER (objective auditory evoked response) and PTA (clinical aurimeter) test were administered (Neurosoft, Russia). The whole procedure was carried out by an audiologist and an ENT specialist.

Results
The PTA results showed that the patient had mild hearing impairment overall, with the loss being more severe in the left ear than in the right. The OAE test results revealed that in-ear of the left side, 84.5% of subjects passed and 15.5% of subjects struggled and were referred to an ear specialist, whereas in the right ear, 82.7% of subjects passed and 17.3% struggled and were referred to an ear specialist. According to this study, in a right-handed study participant, the ear on the left side is more vulnerable than the right side. Differences in the mean hearing threshold at 4000 and 6000 Hz in the left ear were statistically significant in the groups of workers with eleven to fifteen years of practical experience and twenty-one to twenty-five years of practical experience, respectively (Minervini, et al. J Clin Med 12:2652, 2023).

Conclusions
A statistically meaningful threshold shift from 4000 to 6000 Hz is observed as the working experience grows, and this is suggestive of sensorineural hearing impairment brought on by the noisy dental environment.
Assessing the relationship between reproductive autonomy and contraceptive use in rural Malawi
Authors: Wollum Alexandra , Bornstein Marta , Mopiwa Gladson , Norris Alison , Gipson Jessica D. 
Published: September 2023

AbstractReproductive autonomy, or the extent to which people control matters related to their own sexual and reproductive decisions, may help explain why some people who do not intend to become pregnant nevertheless do not use contraception. Using cross-sectional survey data from 695 women aged 16 to 47 enrolled in the Umoyo Wa Thanzi (UTHA) study in Malawi in 2019, we conducted confirmatory factor analysis, descriptive analyses, and multivariable logistic regression to assess the freedom from coercion and communication subscales of the Reproductive Autonomy Scale and to examine relationships between these components of reproductive autonomy and current contraceptive use. The freedom from coercion and communication subscales were valid within this population of partnered women; results from a correlated two-factor confirmatory factor analysis model resulted in good model fit. Women with higher scores on the freedom from coercion subscale had greater odds of current contraceptive use (aOR 1.13, 95% CI: 1.03–1.23) after adjustment for pregnancy intentions, relationship type, parity, education, employment for wages, and household wealth. Scores on the communication subscale were predictive of contraceptive use in some, but not all, models. These findings demonstrate the utility of the Reproductive Autonomy Scale in more holistically understanding contractive use and non-use in a lower-income setting, yet also highlight the need to further explore the multidimensionality of women’s reproductive autonomy and its effects on achieving desired fertility.
Effects and action mechanisms of individual cytokines contained in PRP on osteoarthritis
Authors: Wang Zhengchao , Zhu Pengfei , Liao Bokai , You Hongbo , Cai Yu 
Published: September 2023

AbstractOsteoarthritis (OA) is defined as a degenerative joint disease that can affect all tissues of the joint, including the articular cartilage, subchondral bone, ligaments capsule, and synovial membrane. The conventional nonoperative treatments are ineffective for cartilage repair and induce only symptomatic relief. Platelet-rich plasma (PRP) is a platelet concentrate derived from autologous whole blood with a high concentration of platelets, which can exert anti-inflammatory and regenerative effects by releasing multiple growth factors and cytokines. Recent studies have shown that PRP exhibits clinical benefits in patients with OA. However, high operational and equipment requirements greatly limit the application of PRP to OA treatment. Past studies have indicated that high-concentration PRP growth factors and cytokines may be applied as a commercial replacement for PRP. We reviewed the relevant articles to summarize the feasibility and mechanisms of PRP-based growth factors in OA. The available evidence suggests that transforming growth factor-α and β, platelet-derived growth factors, epidermal growth factor, insulin-like growth factor-1, and connective tissue growth factors might benefit OA, while vascular endothelial growth factor, tumor necrosis factor-α, angiopoietin-1, and stromal cell derived factor-1α might induce negative effects on OA. The effects of fibroblast growth factor, hepatocyte growth factor, platelet factor 4, and keratinocyte growth factor on OA remain uncertain. Thus, it can be concluded that not all cytokines released by PRP are beneficial, although the therapeutic action of PRP has a valuable potential to improve.
COVID-19 associated Mucormycosis among ICU patients: risk factors, control, and challenges
Authors: Abd El-Baky Rehab Mahmoud , Shady Esraa R. , Yahia Ramadan , Ahmed Fatma Y. , Ramadan Mohamed , Ahmed Hala Rady , Al-Kadmy Israa M. S. , Ramadan Yasmin N. , Hetta Helal F. 
Published: September 2023

AbstractThe severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) pandemic is still difficult to be controlled. The spread of this virus and the emergence of new variants are considered a great challenge worldwide. Disturbance in infection control guidelines implementation, use of steroids, antibiotics, hospital crowdedness, and repeated use of oxygen masks during the management of critically ill COVID-19 patients lead to an increase in the rate of opportunistic infections. So, patients need to fight both the virus with its different variants and opportunistic pathogens including bacteria and fungi especially patients with diabetes mellitus, malignancy, or those who undergo hemodialysis and receive deferoxamine. During the pandemic, many cases of Mucormycosis associated with COVID-19 infection were observed in many countries. In this review, we discuss risk factors that increase the chance of infection by opportunistic pathogens, especially fungal pathogens, recent challenges, and control measures.
Identification and experimental validation of autophagy-related genes in abdominal aortic aneurysm
Authors: Yuan Xiaoli , Song Yancheng , Xin Hai , Zhang Lu , Liu Bingyu , Ma Jianmin , Sun Ruicong , Guan Xiaomei , Jiang Zhirong 
Published: September 2023

Abstract
Aim
Autophagy plays essential roles in abdominal aortic aneurysm (AAA) development and progression. The objective of this study was to verify the autophagy-related genes (ARGs) underlying AAA empirically and using bioinformatics analysis.

Methods
Two gene expression profile datasets GSE98278 and GSE57691 were downloaded from the Gene Expression Omnibus (GEO) database, and principal component analysis was performed. Following, the R software (version 4.0.0) was employed to analyze potentially differentially expressed genes related with AAA and autophagy. Subsequently, the candidate genes were screened using protein–protein interaction (PPI), gene ontology (GO) enrichment analysis, and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis. Finally, quantitative real-time polymerase chain reaction (RT-qPCR) was performed to detect the RNA expression levels of the top five selected abnormal ARGs in clinical samples obtained from the normal and AAA patients.

Results
According to the information contained (97 AAA patients and 10 healthy controls) in the two datasets, a total of 44 differentially expressed autophagy-related genes (6 up-regulated genes and 38 down-regulated genes) were screened. GO enrichment analysis of differentially expressed autophagy-related genes (DEARGs) demonstrated that some enrichment items were associated with inflammation, and PPI analysis indicated interaction between these genes. RT-qPCR results presented that the expression levels of IL6, PPARG, SOD1, and MAP1LC3B were in accordance with the bioinformatics prediction results acquired from the mRNA chip.

Conclusion
Bioinformatics analysis identified 44 potential autophagy-related differentially expressed genes in AAA. Further verification by RT- qPCR presented that IL6, PPARG, SOD1, and MAP1LC3B may affect the development of AAA by regulating autophagy. These findings might help explain the pathogenesis of AAA and be helpful in its diagnosis and treatment.
Panobinostat (LBH589) combined with AM1241 induces cervical cancer cell apoptosis through autophagy pathway
Authors: Sheng Bo , Wang Wenwen , Xia Dongyue , Qu Xiangdong 
Published: September 2023

Abstract
Purpose
The study aims to investigate the apoptotic effects of combining LBH589 and AM1241 (a selective CB2 receptor agonist) on cervical cancer cells and elucidating the mechanism of this combined therapy, which may provide innovative strategies for treating this disease.

Methods
The viability of the cervical cancer cells was measured by cell counting kit-8 (CCK-8) assay, and the synergistic effect was analyzed using SynergyFinder. Cell proliferation was tested by cell cloning. The apoptosis and reactive oxygen species (ROS) production in cervical cancer cells were analyzed by flow cytometry. Western blot and quantitative real-time PCR (qRT-PCR) were employed to determine changes in protein and gene levels of pathway-related factors.

Results
By the results of cytotoxicity assay, SiHa cells were selected and treated with 0.1 μM LBH589 and 4 μM AM1241 for 24 h for subsequent experiments. The combination of both was synergistic as determined by bliss, ZIP, HSA and LOEWE synergy score. Plate cloning results showed that LBH589 combined with AM1241 inhibited the proliferation of cervical cancer cells compared to individual drug. Additionally, compared with LBH589 alone, the combination of LBH589 and AM1241 induced autophagy by increasing LC3II/LC3I and decreasing P62/GAPDH, leading to a significantly higher rate of apoptosis. Pharmacological inhibition of also inhibited apoptosis. Consistently, we found that the endoplasmic reticulum, DNA damage repair pathway were induced after co-administration. Furthermore, cellular ROS increased after co-administration, and apoptosis was inhibited by the addition of ROS scavenger.

Conclusion
LBH589 combined with AM1241 activated the endoplasmic reticulum emergency pathway, DNA damage repair signaling pathway, oxidative stress and autophagy pathway, ultimately promoting the apoptosis of cervical cancer cells. These findings suggest that the co-administration of LBH589 and AM1241 may be a new treatment plan for the treatment of cervical cancer.
Figurant types in scenario-based exercises
Authors: Jonsson Cecilia , Lif Susanna 
Published: September 2023

AbstractScenario-based exercises employing figurants are frequently used in vocational education worldwide. The purpose of these exercises is to help the students develop practical knowledge and skills on their way to becoming professional practitioners. While previous research often concentrates on the exercises’ design, level of realism and complexity, there is a knowledge gap regarding the use of figurants, not least when it comes to advantages and disadvantages with different figurant types. The aim of this study is to investigate how, why and in what type of exercises figurants are used in Swedish police education. The empirical material derives from interviews with teachers, students, and figurants from five Swedish police education programmes, focusing on the interviewees’ experience of the use of internal, external, and semi-external figurants in distinctive exercises. The three figurant types were attributed specific effects on the implementation of exercises and were perceived as having different advantages and disadvantages depending on the purpose of each exercise, the impact being particularly noticeable in relation to levels of realism, permissive environments, the counterpart perspective, safety concerns, feedback, and simulation competence. The choice of figurant type has an equally important impact on the outcome of the scenarios as other factors, and there is a need for more research on the use of figurants in relation to student learning.
Staphylococcus aureus contamination of animal-derived foods in Nigeria: a systematic review, 2002—2022
Authors: Odetokun Ismail Ayoade , Adetona Mutiat Adenike , Ade-Yusuf Rodhiat Oyinlola , Adewoye Adeola Oluwafunmibi , Ahmed Aliyu Nuhu , Ghali-Mohammed Ibrahim , Al-Mustapha Ahmed Ibrahim , Fetsch Alexandra 
Published: September 2023

Abstract
Background
Staphylococcus aureus (S. aureus) is a bacterium of public health importance. The zoonotic spread of this pathogen through animal-derived foods has been reported. This systematic literature review investigates the prevalence, distribution, antimicrobial resistance (AMR) profiles, and molecular characteristics of S. aureus in the food chain in Nigeria.

Methods
A systematic search of online databases (Pub Med, Google Scholar, and Web of Science) for published articles from January 2002 to January 2022 was performed using the Prisma guideline.

Results
Fifty articles were included from an initial 511 extracted documents. These papers included research carried out in 22 states across Nigeria. S. aureus detection in most studies was above the satisfactory level for foods (≥ 104 CFU/g). The prevalence of S. aureus ranged from 1.3% in raw cow meat to 72.5% in fresh poultry meat. Most S. aureus isolates demonstrated multiple drug resistance patterns, especially being resistant to beta-lactams. There is a lack of information on the molecular typing of the S. aureus isolates. The different spa types of S. aureus isolated were t091, t314, t1476, and t4690, categorized into Multi-Locus-Sequence Types ST8, ST121, ST152, and ST789. Virulence genes detected include pvl, sea, see, spa, coa, edin, tsst, and hly. Certain AMR-encoding genes were detected, such as mecA, blaZ, fos, tet, and dfsr.. Factors contributing to the presence of S. aureus were reported as poor processing, poor sanitary conditions of the food processing units, inadequate storage units, and poor handling.

Conclusion
We showed that S. aureus is a major food contaminant in Nigeria despite the need for more information on the molecular typing of strains from animal-derived food sources. There is a need to control S. aureus by targeting specific entry points based on the findings on risk factors and drivers of food contamination.
Performance study of a 360° CZT camera for monitoring 177Lu-PSMA treatment
Authors: Vergnaud Laure , Badel Jean-Noël , Giraudet Anne-Laure , Kryza David , Mognetti Thomas , Baudier Thomas , Rida Hanan , Dieudonné Arnaud , Sarrut David 
Published: September 2023

Abstract
Background
The aim of this study was to investigate the quantification performance of a 360° CZT camera for 177Lu-based treatment monitoring.

Methods
Three phantoms with known 177Lu activity concentrations were acquired: (1) a uniform cylindrical phantom for calibration, (2) a NEMA IEC body phantom for analysis of different-sized spheres to optimise quantification parameters and (3) a phantom containing two large vials simulating organs at risk for tests. Four sets of reconstruction parameters were tested: (1) Scatter, (2) Scatter and Point Spread Function Recovery (PSFR), (3) PSFR only and (4) Penalised likelihood option and Scatter, varying the number of updates (iterations × subsets) with CT-based attenuation correction only. For each, activity concentration (ARC) and contrast recovery coefficients (CRC) were estimated as well as root mean square. Visualisation and quantification parameters were applied to reconstructed patient image data.

Results
Optimised quantification parameters were determined to be: CT-based attenuation correction, scatter correction, 12 iterations, 8 subsets and no filter. ARC, CRC and RMS results were dependant on the methodology used for calculations. Two different reconstruction parameters were recommended for visualisation and for quantification. 3D whole-body SPECT images were acquired and reconstructed for 177Lu-PSMA patients in 2–3 times faster than the time taken for a conventional gamma camera.

Conclusion
Quantification of whole-body 3D images of patients treated with 177Lu-PSMA is feasible and an optimised set of parameters has been determined. This camera greatly reduces procedure time for whole-body SPECT.
Dual degree branched type-2 fuzzy controller optimized with a hybrid algorithm for frequency regulation in a triple-area power system integrated with renewable sources
Authors: Kumari Nisha , Aryan Pulakraj , Raja G. Lloyds , Arya Yogendra 
Published: September 2023

AbstractThe uncertainties associated with multi-area power systems comprising both thermal and distributed renewable generation (DRG) sources such as solar and wind necessitate the use of an efficient load frequency control (LFC) technique. Therefore, a hybrid version of two metaheuristic algorithms (arithmetic optimization and African vulture's optimization algorithm) is developed. It is called the ‘arithmetic optimized African vulture's optimization algorithm (AOAVOA)’. This algorithm is used to tune a novel type-2 fuzzy-based proportional–derivative branched with dual degree-of-freedom proportional–integral–derivative controller for the LFC of a three-area hybrid deregulated power system. Thermal, electric vehicle (EV), and DRG sources (including a solar panel and a wind turbine system) are connected in area-1. Area-2 involves thermal and gas-generating units (GUs), while thermal and geothermal units are linked in area-3. Practical restrictions such as thermo-boiler dynamics, thermal-governor dead-band, and generation rate constraints are also considered. The proposed LFC method is compared to other controllers and optimizers to demonstrate its superiority in rejecting step and random load disturbances. By functioning as energy storage elements, EVs and DRG units can enhance dynamic responses during peak demand. As a result, the effect of the aforementioned units on dynamic reactions is also investigated. To validate its effectiveness, the closed-loop system is subjected to robust stability analysis and is compared to various existing control schemes from the literature. It is determined that the suggested AOAVOA improves fitness by 40.20% over the arithmetic optimizer (AO), while frequency regulation is improved by 4.55% over an AO-tuned type-2 fuzzy-based branched controller.
Dietary assessment across various life stages of seven-spotted lady beetle Coccinella septempunctata (Coleoptera: Coccinellidae)
Authors: Abbas Sohail , Abbas Muneer , Alam Aleena , Feng Xiao , Raza Ali , Shakeel Muhammad , Qin Weibo , Han Xiao , Chen Rizhao 
Published: September 2023

Abstract
Background
Coccinella septempunctata (L.) is an important predator in fighting against aphids on edible crops and vegetables. However, to establish a successful mass-rearing technique, it is crucial to determine the optimal diets for larvae, pupae, and adults in terms of fecundity, longevity, and ingredient ratios. The current study aims to learn more about the biology of ladybird beetle using both artificial and natural food components.

Results
This study tested six different ratios of natural and artificial diets, ranging from a natural aphid diet to a variety of artificial diets containing protein hydrolyzate, casein protein, honey, dry aphids, yeast, and agar. The results showed that the natural diet (D1) produced from aphids showed approximately 290.2 eggs, while artificial diets D2 and D4 produced 56.5 and 40.2 eggs, respectively. The effect of different diets on the longevity of different life stages was also tested. The minimum longevity of egg, larval, pupal, and adult stages were recorded for D1 and D4. Furthermore, the maximum percentage emergence of larval, pupal, and adult stages was observed for D1 and D2. D6 produced the longest adult duration, with a maximum of 84.60 days. However, there was no significant difference in sex ratio among the various diet components.

Conclusions
In conclusion, the results suggest that artificial diets are essential for sustaining C. septempunctata cultures in laboratories, especially during periods when natural prey is scarce. The most effective and efficient mass-rearing approach would be to provide both natural and artificial foods simultaneously. These findings may have significant implications for the development of biological control strategies for aphids in agriculture.
Sustainable Integration of US Food and Drug Administration–Approved Biosimilars: Pharmacy- Versus Physician-Driven Change
Authors: Waterhouse David M. , Ward Patrick , Drosick David R. , Burdette Caleb , Davies Daniel , Mendenhall Molly A. 
Published: September 2023

PURPOSE Biosimilars are clinically equivalent to branded products yet cost significantly less. Interchangeability is a US Food and Drug Administration (FDA) designation that allows generic drugs to be substituted for reference drugs at the pharmacy, without a physician's consent. Currently, no oncologic biosimilar has FDA approval for interchangeability. METHODS Building on pharmacy auto-substitution processes with therapeutic interchange, Plan-Do-Study-Act methodology was used to automate conversions from reference biological products to Pharmacy and Therapeutics–/Physician-approved biosimilars. After establishing the baseline metrics, cycle 1 focused on full staff education (completed July 2020) with systematic pharmacy-driven biosimilar conversion initiated in September 2020 for rituximab, trastuzumab, and bevacizumab. Physician-initiated conversion of Neulasta biosimilar products was encouraged but not mandated. During cycle 2 (May 1, 2021-November 30, 2021), pharmacy-driven Neulasta biosimilar conversion was mandated. In cycle 3 (December 1, 2021-April 30, 2023), stakeholder education was reinforced and the sustainability of conversions was confirmed. RESULTS Systematic pharmacy-driven conversion to biosimilar products improved over cycles 1 and 2 from baseline: 1.8% to 90.3% for rituximab, 9.2% to 89.7% for trastuzumab, and 20.5% to 96.1% for bevacizumab. Physician-driven biosimilar conversion for Neulasta was lower at 12.7% through April 2021. Pharmacy-driven Neulasta biosimilar conversion was initiated during cycle 2, resulting in a conversion rate of 39.7%. The conversion rates remained sustainable through April 2023. CONCLUSION Pharmacy-driven auto-substitution of biosimilar products results in rapid and statistically significant biosimilar adoption. The pharmacy-based substitution approach was found to be far more effective than physician-driven substitution. Rapid conversion from branded products to FDA-approved biosimilar is feasible, measurable, and sustainable and can be scaled. Barriers to Neulasta conversion warrant further investigation.