bluuebunny/crossref_metadata_2025_split huggingface dataset:
Systemic deuteration of SCID mice using the water‐isotopologue deuterium oxide (D2O) inhibits tumor growth in an orthotopic bioluminescent model of human pancreatic ductal adenocarcinoma
Authors: Jandova Jana , Galons Jean‐Philippe , Dettman David L. , Wondrak Georg T. 
Published: February 2023

AbstractSince its initial discovery as a natural isotopologue of dihydrogen oxide (1H2O), extensive research has focused on the biophysical, biochemical, and pharmacological effects of deuterated water (2H2O [D2O, also referred to as “heavy water”]). Using a panel of cultured human pancreatic ductal adenocarcinoma (PDAC) cells we have profiled (i) D2O‐induced phenotypic antiproliferative and apoptogenic effects, (ii) redox‐ and proteotoxicity‐directed stress response gene expression, and (iii) phosphoprotein‐signaling related to endoplasmic reticulum (ER) and MAP‐kinase stress response pathways. Differential array analysis revealed early modulation of stress response gene expression in both BxPC‐3 and PANC‐1 PDAC cells elicited by D2O (90%; ≤6 h; upregulated: HMOX1, NOS2, CYP2E1, CRYAB, DDIT3, NFKBIA, PTGS1, SOD2, PTGS2; downregulated: RUNX1, MYC, HSPA8, HSPA1A) confirmed by independent RT‐qPCR analysis. Immunoblot‐analysis revealed rapid (≤6 h) onset of D2O‐induced MAP‐kinase signaling (p‐JNK, p‐p38) together with ER stress response upregulation (p‐eIF2α, ATF4, XBP1s, DDIT3/CHOP). Next, we tested the chemotherapeutic efficacy of D2O‐based drinking water supplementation in an orthotopic PDAC model employing firefly luciferase‐expressing BxPC‐3‐FLuc cells in SCID mice. First, feasibility and time course of systemic deuteration (30% D2O in drinking water; 21 days) were established using time‐resolved whole‐body proton magnetic resonance imaging and isotope‐ratio mass spectrometry‐based plasma (D/H)‐analysis. D2O‐supplementation suppressed tumor growth by almost 80% with downregulated expression of PCNA, MYC, RUNX1, and HSP70 while increasing tumor levels of DDIT3/CHOP, HO‐1, and p‐eIF2α. Taken together, these data demonstrate for the first time that pharmacological induction of systemic deuteration significantly reduces orthotopic tumor burden in a murine PDAC xenograft model.
Group‐buying with products of heterogeneous quality
Authors: Song Hui , Ma Hongkun , Ma Zimeng 
Published: February 2023

AbstractThe present paper proposes a model with vertical differentiation to study firms' incentives using group‐buying. We look into two cases. In the first study, firms are restricted to use the same threshold. If the low‐tech firm uses group‐buying, the high‐tech firm follows suit. The reverse does not necessarily hold but rather depends on parameters. In the second study, this restriction is relaxed. We find that the high‐tech firm tends to impose higher threshold than the low‐tech firm. The reason is that the price effect from using group‐buying is stronger for the high‐tech firm than the low‐tech firm.
Access to healthcare for people with sickle cell disease: Views of healthcare professionals on policies and practices
Authors: Adigwe Obi Peter 
Published: February 2023

AbstractIntroductionSickle cell disease is a genetic disorder with its highest prevalence in Nigeria. The condition causes red blood cells to turn rigid, and consequently, results to several complications including organ damage. This study aimed at assessing views of health practitioners in Nigeria on policies and practices in the area of access to healthcare services for sickle cell disease.MethodsA cross‐sectional study was undertaken amongst healthcare professionals in Nigeria. Data were collected using a self‐administered questionnaire, and analyses were undertaken using Statistical Package for Social Sciences software.ResultsA significant proportion of the participants (42.8%) disagreed that relevant legislative framework exists to facilitate optimal access to high‐quality healthcare services for persons with sickle cell disorder in Nigeria. Two‐thirds of the study cohort were of the opinion that public health surveillance towards sickle cell disease was suboptimal (61.2%). Also, more than three‐quarters of the respondents (78.7%) indicated that the cost of managing sickle cell disease was not affordable to majority of affected Nigerians.ConclusionThis study provides critical insights into access to healthcare services for sickle cell disease. As such, challenges preventing access to healthcare services for sickle cell patients which have been identified in this study can underpin the development of contextual policies to address them.
Dissecting operant alcohol self‐administration using saccharin‐fading procedure
Authors: Calleja‐Conde Javier , Echeverry‐Alzate Víctor , Bühler Kora‐Mareen , Morales‐García Jose Ángel , Segovia‐Rodríguez Lucía , Durán‐González Pedro , Olmos Pedro , de Fonseca Fernando Rodríguez , Giné Elena , López‐Moreno Jose Antonio 
Published: February 2023

AbstractBackgroundAlthough alcohol use disorder is a complex human pathology, the use of animal models represents an opportunity to study some aspects of this pathology. One of the most used paradigms to study the voluntary alcohol consumption in rodents is operant self‐administration (OSA).AimsIn order to facilitate the performance of this paradigm, we aim to describe some critical steps of OSA under a saccharin‐fading procedure.Material & MethodsWe used 40 male Wistar rats to study the process of acquiring the operant response through a saccharin‐fading procedure under a fixed ratio (FR1) schedule of reinforcement. Next, we analyze the alcohol introduction and concentration increase, the effect of an alcohol deprivation, and the analogy between this paradigm with the Drinking in the Dark‐Multiple Scheduled Access paradigm.ResultsDuring alcohol concentration increase, animals reduced their lever presses in accordance with the increase in alcohol concentration. On the contrary, the consumption measured in g·kg−1 BW showed a great stability. The lever presses pattern within operant session changes with the introduction of different alcohol concentrations: at higher alcohol concentrations, animals tended to accumulate most of their presses in the initial period of the session.DiscussionWe show the utility of fading with low concentrations of saccharin and the evolution of the operant response through the different concentrations of alcohol.ConclusionTaken together, our results aimed to dissect the acquisition and maintenance of OSA behavior as well as other related variables, to facilitate the understanding and performance of this paradigm.
Equivocal end‐of‐therapy imaging findings do not predict a higher risk of local relapse after definitive radiotherapy in pediatric Ewing sarcoma and rhabdomyosarcoma
Authors: McLean‐Thomas Lorna , Gao Dexiang , Trenbeath Zachary , Cost Carrye R. , Milgrom Sarah A. 
Published: February 2023

AbstractBackgroundPosttherapy imaging studies can provide reassurance or induce anxiety regarding risk of recurrence for patients and their families. In some cases, it is difficult to determine if imaging findings represent posttreatment changes or residual disease. Equivocal radiographic findings can occur due to therapy‐related inflammation or residual, inactive soft tissue masses, but it is unknown if such findings indicate an increased likelihood of local recurrence. The aim of this study was to assess the value of initial posttherapy scans for predicting local relapse in patients with Ewing sarcoma (EWS) or rhabdomyosarcoma (RMS) who received radiotherapy (RT) for local control. These findings are critical to inform clinicians’ surveillance recommendations and ability to accurately counsel patients and their families.ProcedureThe primary endpoint was time to local progression (LP). Patients were classified as having posttherapy scans that were “positive” (residual disease within the RT field), “negative” (no evidence of residual disease within the RT field), or “equivocal” (no determination could be made). The value of initial posttreatment scans for predicting LP was assessed using positive predictive value (PPV) and negative predictive value (NPV).ResultsNegative imaging findings (n = 51) had an NPV of 88%, and positive imaging findings (n = 1) had a PPV of 100%. When equivocal findings (n = 16) were categorized with negative results (i.e., positive vs. equivocal/negative), the NPV was 90%. When equivocal findings were categorized with positive results (equivocal/positive vs. negative), the PPV was 12%.ConclusionEquivocal findings within the RT field on end‐of‐therapy imaging studies indicate no higher risk of local recurrence than negative findings. These results may contribute to appropriate surveillance schedules and accurate counseling of patients with RMS and EWS who have received RT for local control.
Impact of using fasting plasma glucose and HbA1c instead of OGTT as a screening tool for gestational diabetes: a retrospective study
Authors: Cooke Hannah , Craig Stephen , Kahal Hassan , Talbot Fleur , Lonnen Kathryn 
Published: February 2023

AbstractAims: To examine the impact of using the Royal College of Obstetricians and Gynaecologists (RCOG) COVID‐19 Gestational Diabetes (GDM) criteria on identifying women with GDM.Methods: Data were collected between November 2018 and June 2019, from women previously diagnosed with GDM using NICE guidelines, who also had HbA1c data available. These were used to determine whether they would have been diagnosed had the RCOG criteria been applied. Data were also collected to compare birth and fetal outcomes, and to compare medical management.Results: Eighty‐nine women were included; 43 (48%) met both the RCOG and NICE GDM diagnostic criteria (RCOG + NICE group), while 46 (52%) only met the NICE GDM criteria and would have been missed using the RCOG GDM criteria (NICE only group). There were no significant differences in outcomes between the groups in terms of neonatal and delivery complications. More women in the RCOG + NICE group required treatment with insulin (11 [25.6%] vs 1 [2.2%], p=0.0010) or metformin (23 [53.5%] vs 14 [30.4%], p=0.03).Conclusions: Applying the RCOG criteria resulted in approximately half of women previously diagnosed with GDM to be missed, one‐third of whom had severe insulin resistance requiring treatment with metformin or insulin. The retrospective design of this study may not represent the neonatal and delivery outcomes these women may have had if their diagnosis had been missed. Women should be informed about this risk when not offered an oral glucose tolerance test to guide decision making. Copyright © 2023 John Wiley & Sons.
Improving diabetes care in Ealing, London: an analysis of the NDA and diabetes‐related admissions data during first year of COVID‐19
Authors: Chandok Raj , Baynes Kevin , Birnbaum Maurice , Vanterpool Grace , Okwu Adamma , Bains Kanchan , Sidhu Diljit , Dhatt Mini , Mehar Salma , Chitra Baldeep 
Published: February 2023

AbstractIn this paper, we present and analyse National Diabetes Audit (NDA) and diabetes‐related emergency admissions data in Ealing during the period 1 January 2020 to 31 March 2021. Care for diabetes and other long‐term conditions was disrupted and significantly impacted during this initial period of the COVID‐19 pandemic.The NDA analysis shows that for type 1 and type 2 diabetes Key Care Processes (KCPs), both the eight and nine KCPs fell between 2019/20 and 2020/21, but the relative fall for NHS Ealing was lower than that seen for England. Type 1 diabetes Three Treatment Targets (3TTs) for NHS Ealing increased from 22.6% in 2019/20 to 26.4% in 2020/21; in contrast the 3TTs for England increased slightly from 19.8% in 2019/20 to 20.8% in 2020/21. Type 2 diabetes 3TTs for NHS Ealing changed from 40.9% in 2019/20 to 38.9% in 2020/21, while in England it was 40.3% in 2019/20 and 35.5% in 2020/21.The diabetes‐related emergency admissions analysis shows that there were reductions in the number and rate of emergency admissions for cerebrovascular accident and myocardial infarction; admissions and rates for diabetic ketoacidosis and amputations were the same; those for diabetes mellitus and hypoglycaemia increased. There were overall cost savings of £874,147 due to estimated avoided admissions.In Ealing, the NDA data, diabetes‐related emergency admissions and estimated avoided admissions data show that improvements in diabetes care achieved in previous years in Ealing, faltered, but were broadly sustained in the first pandemic year. Support from the Ealing diabetes care teams, improved self‐management of diabetes and the empowering of people with diabetes through digital technologies could explain these trends in Ealing. Continued access to health care practitioners during the COVID‐19 pandemic is important to ensure the appropriate management of long‐term conditions such as type 1 diabetes mellitus and type 2 diabetes mellitus. Copyright © 2023 John Wiley & Sons.
Conformational and binding mode assessment of the human IL‐3 recognition by its alpha receptor
Authors: Fogha Jade , de Brevern Alexandre G. , Diharce Julien 
Published: February 2023

AbstractProtein–protein interactions (PPIs) are attractive targets as they are critical in a variety of biological processes and pathologies. As an illustration, the interleukin 3 (IL‐3) and its α subunit receptor (IL‐3Rα) are two proteins belonging to the cytokine or receptor βc family and are involved in several disorders like inflammatory diseases or hematological malignancies. This PPI exhibits a low binding affinity and a complex formed by a mutant form of IL‐3 (superkine) and IL‐3Rα have emerged from the literature, with an increase of the affinity. Therefore, in this study, we performed molecular dynamics simulations and binding energy calculation in order to evaluate protein dynamics and to characterize the main interactions between IL‐3 and IL‐3Rα, considering both wild‐type and mutant. First, in the case of IL‐3Rα/IL‐3 wild‐type complex, IL‐3Rα can adopt three different conformations essentially driven by NTD domain, including the open and closed conformations, previously observed in crystal structures. Additionally, our results reveal a third conformation that has a distinct interaction profile that the others. Interestingly, these conformational changes are attenuated in IL‐3Rα/IL‐3 mutant complex. Then, we highlighted the contribution of different residues which interact principally with IL‐3 or IL‐3Rα conserved region. As for the mutated residue at position 135 of IL‐3, other residues such as IL‐3 E138, IL‐3 D40, IL‐3Rα Y279, IL‐3Rα K235, or IL‐3Rα R277 seem important for a low or a high binding affinity. Altogether these findings yield new information that could be exploited in a drug discovery process.
A Phenolic Schiff Based AIE‐Active Quinoxaline‐Based Receptor for Selective Sensing of Fluoride Ions
Authors: Zalmi Geeta A. , Jadhav Sandeep E. , Mirgane Harshad A. , Madje Balaji R. , Bhosale Sheshanath V. 
Published: February 2023

AbstractAnions play very important role in various physiological, biological process and essential in various medical diagnosis. Amongst, anionic species fluoride is considered an essential component, however, the excess amount of fluoride may cause issues with various organs of the body such as kidney and nervous system. Herein we developed a new fluorescent chemosensor based on quinoxaline receptor 1 for rapid sensitive detection of fluoride ion. The receptor contains the free hydroxy proton which easily undergoes deprotonation occurs the OH− to give phenoxide ion via hydrogen bond interaction with fluoride ions. The deprotonation reaction on the aromatic system. The receptor 1 showed good selectivity towards F− ion in presence over another anion. The absorption and emission studies, naked eye detection and test strip application was performed for fluoride ion detection. The binding constant for the receptor was calculated by Benesi Hildebrand method and was found to be 8.76×104 M−1. While the limit of detection was observed to be 3.1 μM which much lower than the permissible limit of 270 μM given by United State Environmental Protection Agency (EPA). Thus, we believe this new derivative may be used in future for real world application for fluoride ion detection.
Efficient Conversion of Biomass‐Derived Furfural to Tetrahydrofurfuryl Alcohol over Co3O4−C Nanocomposite Catalyst at Mild Conditions
Authors: Van Nguyen Chi , Le Giau M. , Nguyen Tung T. , Van Tran Thuan , Vu Thanh Dien To , Nguyen Phuong‐Tung , Ngoc Tran Quyen , Phan Nam T. S. 
Published: February 2023

AbstractDue to the depletion of fossil fuel resources, catalytic conversion of biomass into liquid fuels and valuable compounds has become an attractive field of research. Tetrahydrofurfuryl alcohol (THFAL) is an important platform molecule in both industrial and pharmacological fields, and catalytic hydrogenation of furfural (FA) to THFAL is a sustainable process. However, an active and stable catalyst for the direct hydrogenation of FA to THFAL under milder conditions is still a challenge. Accordingly, herein, we investigated a high‐valence metal catalyst (Co3O4−C) for the hydrogenation of FA to THFAL using sodium borohydride (NaBH4) as the H source. Under optimal reaction conditions (that is, 0.05 g Co3O4−C‐700 in an aqueous solution, 5 mmol NaBH4, 60 °C, and 3 h), 88.5 % THFAL was produced over Co3O4−C‐700. The kinetic study revealed that furfuryl alcohol was generated as an intermediate during the hydrogenation of FA. Additionally, the reaction followed pseudo‐first order kinetics, and the reaction rate constant (k) values were .80, 3.41, 3.45, and 5.55 h−1 at 30, 40, 45, 50, and 60 °C, respectively. From the slopes of the Arrhenius plot, the activation energy was determined to be 22.02 kJ mol−1, indicating high activity of the catalyst for the reaction. Moreover, Co3+ and smaller sizes of Co3O4 nanoparticles contributed to the activity of Co3O4−C‐700. We believe that the metal oxide catalyst developed herein can be used for various biomass conversions.
Cobalt Ferrite Incorporated Ocimum sanctum Nanocomposite Matrix as an Interface for Adsorption of Organic Dyes: A Sustainable Alternative
Authors: Sharma Atul , Rasheed Shoaib , Mangla Divyanshi , Choudhry Arshi , Shukla Sneha , Chaudhry Saif Ali 
Published: February 2023

AbstractThe preparation of Cobalt ferrite‐Ocimum sanctum nanocomposite, referred to as CoFe2O4/OS, has been reported along with its application for the treatment of water containing hazardous organic contaminants. A simple co‐precipitation approach was applied for synthesis of magnetic nanocomposite, and a thorough analysis for its characterization was conducted using several spectroscopic and microscopic techniques. The characterization results showed that the magnetic nanocomposite exhibited substantial functional groups of Ocimum sanctum powder and magnetic ferrite part. The adsorption investigation of nanocomposite for removal of two cationic dyes was carried out with varying parameters, such as pH, contact time, temperature, concentration, and composite dosage. The sorption process was proved feasible and exothermic at the tested temperatures (303–323 K). The sorption data followed Freundlich and Temkin isotherms which recommended the physisorption progression. The kinetic study revealed a pseudo‐second order rate of sorption process for both dye molecules. The facile preparation, economical cost, significant magnetic character, and high sorption capacity will surely construct the water treatment system more competent with the use of CoFe2O4/OS.
4‐Hydroxy Enigmol, a 1‐Deoxyphytosphingolipid that Exhibit Good Activity against Prostate and Colon Cancer
Authors: Kaur Parleen , Sharma Sonia , Goel Akshita , Sharma Purshotam , Agnihotri Navneet , Kaur Ramandeep , Singh Vasundhara 
Published: February 2023

AbstractIn the present work, 4‐hydroxy enigmol (1), an analogue of enigmol which is a known orally bioavailable 1‐deoxysphingolipid, effective against multiple cancer cell lines, has been synthesized with an additional hydroxy group for improved anti‐cancer activity. The additional group in compound (1) is likely to show enhanced activity due to improved cell permeability and hydrophilicity. The key steps involved are stereoselective cross metathesis reaction and OsO4/NMO mediated dihydroxylation. Further, analogues of 4‐hydroxy enigmol (1) with varying chain lengths (2, 3) have also been synthesized using the same synthetic strategy for varying hydrophobicity as an additional structural feature. To support the genesis in the design of compound (1–3), the pharmacokinetic studies and docking analysis has been done for calculating the polar surface area, binding affinity and hydrophobicity/hydrophilicity. The preliminary in‐vitro cytotoxicity on cancer cell lines displayed promising activity against the PC‐3 cell lines and HCT‐116 cell lines.
3D/2D Core/Shell Perovskite Nanocrystals for High‐Performance Solar Cells
Authors: Fu Jie , Liu Jun , Yuan Lin , Pan Qi , Chen Shuhua , Hu Yiqi , Chen Jinxing , Ma Wanli , Zhang Qiao , Liu Zeke , Cao Muhan 
Published: February 2023

AbstractAll‐inorganic lead halide perovskite nanocrystals (NCs) emerge as a rising star in photovoltaic fields on account of their excellent optoelectronic properties. However, it still remains challenging to further promote photovoltaic efficiency due to the susceptible surface and inevitable vacancies. Here, this work reports a 3D/2D core/shell perovskite heterojunction based on CsPbI3 NCs and its performance in solar cells. The guanidinium (GA+) rich 2D nanoshells can significantly passivate surface trap states and lower the capping ligand density, resulting in improved photoelectric properties and carrier transport and diminished nonradiative recombination centers via the hydrogen bonds from amino groups in GA+ ions. Consequently, an outstanding power conversion efficiency (PCE) of up to 15.53% is realized, substantially higher than the control device (13.77%). This work highlights the importance of surface chemistry and offers a feasible avenue to achieve high‐performance perovskite NCs‐based optoelectronic devices.
Manganese Local Environment Modulation via SiO4 Substitution to Boost Sodium Storage Performance of Na4MnCr(PO4)3
Authors: Hou Yan , Liu Qiong , Yang Lin , Hu Jing , Wang Zhenyu , Zhang Xinmiao , Pan Jialiang , Bai Zhengyu , Wang Haijiang , Lu Zhouguang 
Published: February 2023

AbstractManganese‐based Na superionic conductors (NASICONs) Na4MnCr(PO4)3 with three‐electron reaction are attractive cathode materials for sodium‐ion batteries. However, the irreversible distortion of Mn local structure leads to sluggish electrode kinetics, voltage hysteresis, and poor cycling stability. Here, SiO4 is introduced to substitute PO4 to modulate the local environment of Mn to activate the redox activity and stabilize the reversibility of Na4MnCr(PO4)2.9(SiO4)0.1 (NMCP‐Si). A combined experimental and theoretical investigation have been undertaken to reveal the evolution of electronic structures and Na storage properties associated with SiO4 substitution. The NMCP‐Si exhibits much‐enhanced rate capability and cycling stability, being attributed to the unique Jahn‐Teller distortion (Mn3+) that facilitates sodium de/insertion kinetics by optimizing the Na ion diffusion channels. This work addresses the challenge of stabilizing the structure of Mn‐based NASICONs and represents a breakthrough in understanding how to improve the Na+ conductivity by regulating local structure.