bluuebunny/crossref_metadata_2025_split huggingface dataset:
Medicinal‐Chemistry‐Driven Approach to 2‐Substituted Benzoxazole–Estradiol Chimeras: Synthesis, Anticancer Activity, and Early ADME Profile
Authors: Kovács Ferenc , Huliák Ildikó , Árva Hédi , Kiricsi Mónika , Erdős Dóra , Kocsis Marianna , Takács Gergely , Balogh György T. , Frank Éva 
Published: September 2023

AbstractThe efficient synthesis of novel estradiol‐based A‐ring‐fused oxazole derivatives, which can be considered as benzoxazole‐steroid domain‐integrated hybrids containing a common benzene structural motif, is described. The target compounds were prepared from steroidal 2‐aminophenol precursors by heterocycle formation or functional group interconversion (FGI) strategies. According to 2D projection‐based t‐distributed stochastic neighbor embedding (t‐SNE), the novel molecules were proved to represent a new chemical space among steroid drugs. They were characterized based on critical physicochemical parameters using in silico and experimental data. The performance of the compounds to inhibit cell proliferation was tested on four human cancer cell lines and non‐cancerous cells. Further examinations were performed to reveal IC50 and lipophilic ligand efficiency (LLE) values, cancer cell selectivity, and apoptosis‐triggering features. Pharmacological tests and LLE metric revealed that some derivatives, especially the 2‐(4‐ethylpiperazin‐1‐yl)oxazole derivative exhibit strong anticancer activity and trigger the apoptosis of cancer cells with relatively low promiscuity risk similarly to the structurally most closely‐related and intensively studied anticancer agent, 2‐methoxy‐estradiol.
Quantification of Genetic Heterogeneity Using Long‐Read Targeted Individual DNA Molecule Sequencing
Authors: Zhang Yingzi , Chandrasekaran Arun Pandian , Bi Chongwei , Li Mo 
Published: September 2023

AbstractUnderstanding genetic heterogeneity is of paramount importance in unraveling the intricate functioning of biological systems, as it contributes to the diversity of phenotypes of gene‐environment interactions. We have developed a method termed targeted Individual DNA Molecule Sequencing (IDMseq) to accurately quantify genetic heterogeneity within cell populations, even those with rare variants present at low frequencies. IDMseq ensures that each original DNA molecule is distinctively represented by one unique molecule identifier (UMI) group, preventing false UMI groups and enabling precise quantification of allele frequency within the original population. IDMseq is a versatile sequencing technique that combines error correction and long‐read sequencing, enabling sensitive detection of various genetic variants, including single nucleotide variants and large structural variants in both basic and clinical research settings. This protocol provides a comprehensive, step‐by‐step guide to preparing samples and performing IDMseq to determine genetic variations. © 2023 The Authors. Current Protocols published by Wiley Periodicals LLC.Basic Protocol: UMI labeling and amplification of DNASupport Protocol 1: AMPure XP beads cleanupSupport Protocol 2: Suggested data analysis pipeline
Comparative study of univariate and multivariate selection strategies based on an integrated approach applied to oilseed rape breeding
Authors: Oghan Hassan Amiri , Bakhshi Behnam , Rameeh Valiollah , Tabrizi Hossein Zeinalzadeh , Faraji Abolfazl , Ghodrati Gholamreza , Fanaei Hamid Reza , Askari Abdolhossein , Kiani Davood , Payghamzadeh Kamal , Sadeghi Hossein , Danaei Amir Khosro , Kazerani Narjes Khatoon , Afrouzi Mohammad Ali Aghajani Nasab , Dalili Alireza 
Published: September 2023

AbstractChoosing the right method to discover superior genotypes is always one of the major challenges that oilseed rape (Brassica napus L.) researchers deal with. This work aimed to compare the univariate and multivariate selection indices utilizing variance‐based, additive main effects and multiplicative interaction based, best linear unbiased prediction based, and genotype × environment/trait based strategies to determine the best‐performing (regarding stability, yield, and agronomic traits) oilseed rape genotypes. Utilized indices were categorized into five general methods, including univariate performance selection index (UPSI: mean performance), univariate stability selection indices (USSIs: environmental variance, Shukla's stability variance, AMMI stability value, modified AMMI stability value, modified AMMI stability index, and weighted average of absolute scores), multivariate performance selection indices (MPSIs: genotype by trait [GT], genotype by yield × trait [GYT], and ideotype‐design index [FAI‐BLUP]), univariate performance and stability selection indices (UPSSIs: coefficient of variability, harmonic mean of genotypic value [HMGV], relative performance of genotypic value [RPGV], WAASBY [WAASB and mean performance {Y}], and genotype + genotype × environment interaction [GGE]), and multivariate performance and stability selection index (MPSSI: MTSI). Data from 18 oilseed rape genotypes and two checks were obtained from trials conducted at eight locations in Iran during two consecutive crop years from 2018 to 2020. The grain yield (GY) and agronomic traits were evaluated. The significant genotype × environment interaction was found for GY and agronomic traits in the combined analysis of variance. In terms of GY, the selection indices GGE, HMGV, and RPGV (6.51), GYT (6.43), GT (5.35), MTSI (4.95), FAI‐BLUP (4.64), and WAASBY (3.97) achieved better‐than‐average genetic gains (GSs). Using an optimized model of USSIs (O‐USSIs), GS increased from −0.15 to 3.26 on average, which shows the efficiency of O‐USSIs in high‐yielding and stable genotypes selection. Based on Spearman's ranking correlation, a positive relationship was found among MPSIs, MPSSI, UPSSIs, and UPSIs, but not for USSIs. Based on an integrated model of selection indices, genotypes G2, G10, G11, G14, and G20 were detected as superior genotypes with high‐yielding, high‐stability, and desirable agronomic traits. It could be concluded that combining different approaches of genotype selection based on an integrated model can provide better answers to oilseed rape breeders.
Self‐adaptive search optimization‐based vehicle path prediction and traffic light controller in vehicular ad hoc network
Authors: Chauhan Shishir Singh , Kumar Dilip 
Published: September 2023

SummaryIn recent days, VANET is considered as the main hopeful equipment in the system of transportation since traffic congestion arises regularly and thus occurred road accidents very easily. Besides, the network traffic is increasing due to the huge quantity of information generated in region of urban. Therefore, one of the primary challenges faced by Intelligent Transportation System (ITS) is ensuring the accurate transmission of information in both vehicle‐to‐vehicle (V2V) and vehicle‐to‐road (V2R) sensing unit communications. So, here, an adaptive routing controller (ARC) is utilized to improve the data transmission between vehicle and road sensing unit (RSU) and to minimize the traffic density in VANET, and the self‐adaptive search optimization is developed in VANET clustering to prioritize vehicles in the lane, in which the multi‐objective function is intended depending on the energy of the node, acceleration, jitter, priority, velocity, and trust factors. The traffic light control is done to facilitate the effective communication in the network. Thus, the proposed technique is calculated in terms of throughput, jitter, quadratic mean of acceleration (QMA), and spatially distributed travel time (SDTT), which acquired the values of 0.53 s, 36.07 kmph, 3.472 s, and 43.572%, respectively, while using 50 vehicles at 50 s.
Hydrodynamic metamaterials: Principles, experiments, and applications
Authors: Chen Mengyao , Shen Xiangying , Xu Lei 
Published: September 2023

AbstractHydrodynamic metamaterials, a nascent research field, possess immense potential for fluid flow manipulation. With engineered structure design, they offer unparalleled control over fluid behavior beyond the capabilities of conventional methods. In this review, we focus on hydrodynamic metamaterials and provide a comprehensive overview of the current state of this research field. We start by introducing basic theories and principles of hydrodynamic metamaterials and then illustrate the different functions of hydrodynamic metamaterials that have been realized in porous medium flow and Hele‐Shaw flow. Moreover, we also demonstrate the multifunctional metamaterials that have been developed in hydrodynamics. Some research progresses are highlighted due to their promising applications, including drag reduction, microfluidic manipulation, and biological tissue coculture. The review concludes by identifying major challenges and proposing research directions for the future.
Presence of the Eucalyptus snout beetle in Ecuador and potential invasion risk in South America
Authors: Crespo‐Pérez Verónica , Soto‐Centeno J. Angel , Pinto C. Miguel , Avilés Ana , Pruna Washington , Terán Claudia , Barragán Álvaro 
Published: September 2023

AbstractEucalyptus snout beetles are a complex of at least eight cryptic species (Curculionidae: Gonipterus scutellatus complex), native to mainland Australia and Tasmania, that defoliate Eucalyptus trees and are considered important pests. Since the 19th century, three species of the complex have been introduced to other continents. Here, we document the presence of Eucalyptus snout beetles in Ecuador. We used DNA data for species identification and unambiguously demonstrated that the Ecuadorian specimens belong to the species Gonipterus platensis, which has low genetic diversity compared with other species in the complex. We analyzed G. platensis' potential distribution in South America with ecological niche models and found several areas of high to intermediate climatic suitability, even in countries where the pest has not been registered, like Peru and Bolivia. Accurate identification of species in the G. scutellatus complex and understanding of their potential distribution are essential tools for improved management and prevention tactics.
Effects of six‐month creatine supplementation on patient‐ and clinician‐reported outcomes, and tissue creatine levels in patients with post‐COVID‐19 fatigue syndrome
Authors: Slankamenac Jelena , Ranisavljev Marijana , Todorovic Nikola , Ostojic Jelena , Stajer Valdemar , Ostojic Sergej M. 
Published: September 2023

AbstractDietary creatine has been recently put forward as a possible intervention strategy to reduce post‐COVID‐19 fatigue syndrome yet no clinical study so far evaluated its efficacy and safety for this perplexing condition. In this parallel‐group, randomized placebo‐controlled double‐blind trial, we analyzed the effects of 6‐month creatine supplementation (4 g of creatine monohydrate per day) on various patient‐ and clinician‐reported outcomes, and tissue creatine levels in 12 patients with post‐COVID‐19 fatigue syndrome. Creatine intake induced a significant increase in tissue creatine levels in vastus medialis muscle and right parietal white matter compared to the baseline values at both 3‐month and 6‐month follow‐ups (p < .05). Two‐way analysis of variance with repeated measures revealed a significant difference (treatment vs. time interaction) between interventions in tissue creatine levels (p < .05), with the creatine group was superior to placebo to augment creatine levels at vastus medialis muscle, left frontal white matter, and right parietal white matter. Creatine supplementation induced a significant reduction in general fatigue after 3 months of intake compared to baseline values (p = .04), and significantly improved scores for several post‐COVID‐19 fatigue syndrome‐related symptoms (e.g., ageusia, breathing difficulties, body aches, headache, and difficulties concentrating) at 6‐month follow‐up (p < .05). Taking creatine for 6 months appears to improve tissue bioenergetics and attenuate clinical features of post‐COVID‐19 fatigue syndrome; additional studies are warranted to confirm our findings in various post‐COVID‐19 cohorts.
Current perspective on biological properties of plasmacytoid dendritic cells and dysfunction in gut
Authors: Guo Xueran , He Chengwei , Xin Shuzi , Gao Han , Wang Boya , Liu Xiaohui , Zhang Sitian , Gong Fengrong , Yu Xinyi , Pan Luming , Sun Fangling , Xu Jingdong 
Published: September 2023

AbstractPlasmacytoid dendritic cells (pDCs), a subtype of DC, possess unique developmental, morphological, and functional traits that have sparked much debate over the years whether they should be categorized as DCs. The digestive system has the greatest mucosal tissue overall, and the pDC therein is responsible for shaping the adaptive and innate immunity of the gastrointestinal tract, resisting pathogen invasion through generating type I interferons, presenting antigens, and participating in immunological responses. Therefore, its alleged importance in the gut has received a lot of attention in recent years, and a fresh functional overview is still required. Here, we summarize the current understanding of mouse and human pDCs, ranging from their formation and different qualities compared with related cell types to their functional characteristics in intestinal disorders, including colon cancer, infections, autoimmune diseases, and intestinal graft‐versus‐host disease. The purpose of this review is to convey our insights, demonstrate the limits of existing research, and lay a theoretical foundation for the rational development and use of pDCs in future clinical practice.
Telomere dysfunction in Tert knockout mice delays BrafV600E‐induced melanoma development
Authors: Zhang Jinglong , Zhang Fan , Porter Kenneth I. , Dakup Panshak P. , Wang Shuwen , Robertson Gavin P. , Gaddameedhi Shobhan , Zhu Jiyue 
Published: September 2023

AbstractTelomerase activation is a crucial step in melanomagenesis, often occurring because of ultraviolet radiation (UVR)‐induced mutations at the telomerase gene (TERT) promoter and rendering TERT transcription in response to the activated Raf‐MAP kinase pathway by BRAFV600E mutation. Due to the excessively long telomeres in mice, this process does not occur during melanomagenesis in mouse models. To investigate the impact of telomere dysfunction on melanomagenesis, BrafV600E was induced in generations 1 and 4 (G1 and G4) of Tert−/− mice. Our findings revealed that, regardless of UVR exposure, melanoma development was delayed in G4 mice, which had shorter telomeres compared to G1 and wild‐type C57BL/6J (G0) mice. Moreover, many G4 tumors displayed an accumulation of excessive DNA damage, as evidenced by increased γH2A.X staining. Tumors from UVR‐exposed mice exhibited elevated p53 protein expression. Cultured tumor cells isolated from G4 mice displayed abundant chromosomal fusions and rearrangements, indicative of telomere dysfunction in these cells. Additionally, tumor cells derived from UVB‐exposed mice exhibited constitutively elevated expression of mutant p53 proteins, suggesting that p53 was a target of UVB‐induced mutagenesis. Taken together, our findings suggest that telomere dysfunction hampers melanomagenesis, and targeting telomere crisis‐mediated genomic instability may hold promise for the prevention and treatment of melanoma.
Psychological variables linked to hesitation toward vaccination against COVID‐19 among late adolescents and young adults: The role of magical thinking and right‐wing authoritarianism
Authors: Pace Ugo , Buzzai Caterina , Passanisi Alessia 
Published: September 2023

AbstractIntroductionSeveral studies on the predisposition to be subjected to vaccination have shown that vaccine hesitation is a global phenomenon influenced by a lack of knowledge and awareness, as well as perceived risks and benefits. The purpose of this study was to investigate the mediating role of right‐wing authoritarianism in the relationship between magical thinking and positive attitudes toward COVID‐19 vaccines (i.e. trust of vaccine benefits, worries over unforeseen future effects, concerns about commercial profits, preference for natural immunity).MethodThe sample consisted of 201 Italian young adults, 49 males (24.4%), and 152 females (75.6%), aged between 18 and 25. Data were collected during 2022.ResultsThe results confirmed the role of right‐wing authoritarianism as a mediator for magical thinking on positive vaccine attitudes. Findings indicated a positive association between right‐wing authoritarianism and magical thinking and a negative relationship of both the aforementioned variables on positive vaccine attitudes. SEM analyses showed a direct positive association from magical thinking to right‐wing authoritarianism and a direct negative association from right‐wing authoritarianism to trust of vaccine benefits, to low worries over unforeseen future effects, to lack of concerns about commercial profits and to low preference for natural immunity.ConclusionsThe findings highlight that common contribution of odd or magical beliefs and right‐wing authoritarianism may slow the spread of vaccines among late adolescents and young adults. Our findings provide insight on what effective communication with vaccine‐resistant individuals should look like in order to increase the chances of reaching vaccine‐hesitant individuals.
Mind over minutes: The effect of task duration consideration on task delay
Authors: YoungJin Chun Libby , Lembregts Christophe , Van den Bergh Bram 
Published: September 2023

AbstractWould highlighting that a customer review can be completed in 3 min influence a customer's decision to either promptly submit their review or delay it, with the potential risk of forgetting it altogether? Despite the popular approach of using task duration to mitigate task delay, the empirical support for this method is scant. This study investigates the effect of considering task duration (i.e., how long a task may take) on task delay (i.e., postponing the task until later). Across four studies, we demonstrate that making task duration salient decreases the likelihood of postponing a short task that can be achieved in one sitting. This effect occurs because considering task duration strengthens the implemental mindset, but only when task duration information is more evaluable. The findings of this paper suggest an easily implementable method that could customer engagement. Finally, we propose a set of promising avenues for future research.
Treatment deferral for elevated blood pressure at a dental school clinic
Authors: Nath Sapna , Jiang Tien , Barrow Jane , Simon Lisa 
Published: September 2023

AbstractObjectivesCurrent guidelines by the American Dental Association (ADA) recommend deferral of elective dental care for elevated blood pressure. However, it is unknown how frequently this impacts dental treatment. The purpose of this study was to evaluate rates of asymptomatic hypertension and treatment deferral at a dental school clinic.MethodsThis was a retrospective study with data extracted from a chart review of all patients presenting for care at a dental school teaching practice. Differences in dental procedures, the time between visits, and the number of antihypertensive medications were calculated between patients with and without a blood pressure reading exceeding current guidelines for elective treatment.ResultsAmong 26,821 individuals, 1265 had a visit with elevated blood pressure. Blood pressure readings at the next visit were significantly lower (systolic blood pressure 137 [95% confidence interval {CI} 135–138] mmHg, diastolic blood pressure 82 [95% CI 81–83 mmHg], p < 0.001), although only 24 patients reported taking a new medication. Only 4.1% of these patients had a procedure deferred; for those that did, the average intervisit time was 88.2 days (95% CI 77.7–98.7 days).ConclusionsThe majority of patients with blood pressure readings exceeding current ADA recommendations for treatment were treated without evidence of harm. Patients were also unlikely to return to the clinic with new medications for blood pressure after a visit with an elevated blood pressure reading. Oral health providers must weigh the risks and benefits of care deferral and can consider an expanded role in hypertension management in dental settings when caring for patients with elevated blood pressure.
Fast and efficient inversion methods for crack sizing using Bz magnetic signature
Authors: Barrarat Fatima , Bensaid Samir , Rayane Karim , Helifa Bachir , Lefkaier Iben Khaldoun , Zorig Assam 
Published: September 2023

AbstractFast estimation is a critical feature of the proposed modeling approach as a “forward” fast solver that has a key role in solving the “inverse” problem involved in crack sizing, especially for real‐time applications. To this end, two different inversion methods have been proposed to estimate the crack depth. The first one uses an interpolation model of the 3D direct model simulation data. The second approach is based on artificial neural networks (ANN). In this article, the rotating uniform eddy current (RUEC) probe is used to detect the normal magnetic component Bz which is defined as the characteristic signal for reconstructing the crack length and depth concurrently. Using the first approach, The Bz characteristic 3D surface is presented, and this can be modeled by a fitted polynomial interpolation equation. Thus, the crack depth can be inverted using this equation referring to the experimental or simulated Bz signal and the crack length. The ANN is outlined to determine the crack depth based on the simulated Bz signature. The move from the first inversion method to the second was flexible and useful, in which the interpolation model is used as a defect signal generator for fast building of a large and efficient database for ANN training. Both of the proposed methods proved the objectivity and accuracy of the inversion results and offered more robust engineering support for automated NDT, reducing production time and increasing productivity.
Diagnosing the relationship between the Madden–Julian Oscillation and United States severe convective weather outbreaks
Authors: McCormick Andrew , Mercer Andrew E. , Brown Michael E. , Fuhrmann Christopher M. 
Published: September 2023

AbstractThe Madden–Julian Oscillation (MJO) is the leading intraseasonal mode of tropical variability and has been connected to influences in extratropical weather. Recently, the MJO has been gaining traction as a useful teleconnection for extending severe weather outbreak forecast lead time. However, the MJO's intraseasonal, planetary‐scale is far from the meso‐ or micro‐scale severe weather phenomena, making identifying links between the two more difficult. In our study, we use severe weather outbreaks comprising all three main hazards (wind, hail and tornadoes), which are typically linked to a synoptic‐scale system. Outbreaks are devised using kernel density estimation and were used to determine spatial shifts for each of the eight phases of the MJO in each month. A total of 15 months/phases indicated a significant spatial shift. In the spring and summer months (March–August), southerly shifts are more likely to occur in MJO phases 7–8 and 1–3, while northerly shifts are more likely in phases 4–6. In the cool season (September–February), only 3 month/phase groups indicated significant shifts, two shifting west and one north. Composite analysis showed the likely link between the MJO and these outbreak shifts was rooted in Rossby wave breaking and generation of a Rossby wave source induced by the barotropic vorticity advection from the MJO convection. These resulting wave breaks and wave trains led to changes in the storm track (amplitude and location) that resulted in a more favourable synoptic‐scale pattern for severe weather over the United States that deviated from the climatologically favourable region.