bluuebunny/crossref_metadata_2025_split huggingface dataset:
Efficacy and safety of the adenosine administration test in the management of Wolff–Parkinson–White pattern in children with delta waves
Authors: Kogawa Kentaro , Ito Reiji , Hirano Daishi , Hoshino Kenji 
Published: January 2025

AbstractBackground:The Wolff–Parkinson–White pattern is a delta wave frequently detected in school-based cardiovascular screening programs in Japan. Although most children with Wolff–Parkinson–White pattern are asymptomatic, initial symptoms may include syncope or sudden death, necessitating accurate diagnosis and management. Delta waves can also indicate a fasciculoventricular pathway, which poses no risk and does not require management.Methods:We reviewed the medical records of patients referred to our hospital between April 2008 and March 2022 to evaluate the electrocardiographic signs of the Wolff–Parkinson–White pattern. The existence of Wolff–Parkinson–White syndrome and fasciculoventricular pathway were determined based on atrioventricular block and QRS waveform changes after adenosine administration during sinus rhythm.Results:The study cohort included 127 children (65 males; median age: 12.8 years, resting heart rate: 75 beats/min, PR interval: 109 ms, and QRS duration: 101 ms). The adenosine administration test revealed a fasciculoventricular pathway, Wolff–Parkinson–White syndrome, and indeterminate findings in 64, 54, and 9 children, respectively. More than 60% of children with a QRS duration ≤ 120 ms had a fasciculoventricular pathway. Age ≤ 12 years, QRS duration >120 ms, and type A category (children with R/S ratios >1 in lead V1) were identified as independent risk factors for Wolff–Parkinson–White syndrome. No adverse events were observed in any child.Conclusions:The adenosine administration test is safe and feasible for differentiating Wolff–Parkinson–White syndrome from fasciculoventricular pathways and can reduce the unnecessary management of children with fasciculoventricular pathways.
The graded effects of bilingualism and language ability on children’s cross-situational word learning
Authors: Crespo Kimberly , Kaushanskaya Margarita 
Published: January 2025

Abstract
The present study examined whether length of bilingual experience and language ability contributed to cross-situational word learning (XSWL) in Spanish-English bilingual school-aged children. We contrasted performance in a high variability condition, where children were exposed to multiple speakers and exemplars simultaneously, to performance in a condition where children were exposed to no variability in either speakers or exemplars. Results revealed graded effects of bilingualism and language ability on XSWL under conditions of increased variability. Specifically, bilingualism bolstered learning when variability was present in the input but not when variability was absent in the input. Similarly, robust language abilities supported learning in the high variability condition. In contrast, children with weaker language skills learned more word-object associations in the no variability condition than in the high variability condition. Together, the results suggest that variation in the learner and variation in the input interact and modulate mechanisms of lexical learning in children.
Post‐Fire Sediment Yield From a Western Sierra Nevada Watershed Burned by the 2021 Caldor Fire
Authors: East Amy E. , Logan Joshua B. , Dartnell Peter , Dow Helen W. , Lindsay Donald N. , Cavagnaro David B. 
Published: January 2025

AbstractWatershed sediment yield commonly increases after wildfire, often causing negative impacts to downstream infrastructure and water resources. Post‐fire erosion is important to understand and quantify because it is increasingly placing water supplies, habitat, communities, and infrastructure at risk as fire regimes intensify in a warming climate. However, measurements of post‐fire sediment mobilization are lacking from many regions. We measured sediment yield from a forested, heavily managed 25.4‐km2 watershed in the western Sierra Nevada, California, over 2 years following the 2021 Caldor Fire, by repeat mapping of a reservoir where sediment accumulated from terrain with moderate to high soil burn severity. Sediment yield was less than the geochronology‐derived long‐term average in the first year post‐fire (conservatively estimated at 21.8–28.0 t/km2), low enough to be difficult to measure with uncrewed airborne system (UAS) and bathymetric sonar survey methods that are most effective at detecting larger sedimentary signals. In the second year post‐fire the sediment delivery was 1,560–2,010 t/km2, an order of magnitude above long‐term values, attributable to greater precipitation and intensive salvage logging. Hillslope erosion simulated by the Water Erosion Prediction Project (WEPP) model overestimated the measured amount by a factor of 90 in the first year and in the second year by a factor (1.9) that aligned with previously determined model performance in northern California. We encourage additional field studies, and validation of erosion models where feasible, to further expand the range of conditions informing post‐fire hazard assessments and management decisions.
Lower Tropospheric Response to Local Sea Surface Temperature Anomalies: A Numerical Study in the EUREC4 ${\text{EUREC}}^{4}$A Region
Authors: Borgnino M. , Desbiolles F. , Meroni A. N. , Pasquero C. 
Published: January 2025

AbstractOcean and atmosphere exchange energy and mass at their interface at a rate that depends on the dynamical and thermodynamical disequilibrium between water and air. In this work, using an atmospheric model forced by observed high resolution sea surface temperature (SST) fields, we show that the air‐sea disequilibrium is also maintained by atmospheric processes driven by fine scale SST patterns. The analysis focuses on north‐western tropical Atlantic and indicates that fine scale SST anomalies modulate the air column stability and the entrainment of dry air at boundary layer top. The main effect is a modification in near surface temperature and moisture content, which produces a negative response of relative humidity to local SST variability, resulting in a larger sensitivity of latent than sensible heat fluxes to local SST anomalies. Such sensitivities are quantified as 95% and 70% of fluxes upper bound, estimated with local atmospheric properties uncorrelated with SST anomalies.
Direct Observations of North Pacific Subsurface Low Potential Vorticity Water Impinging on the Kuroshio
Authors: Wang Ran , Ren Qiang , Nan Feng , Yu Fei , Chen Zifei , Liu Yansong , Wang Jianfeng , Zhang Chuanzheng , Zhao Ruixiang , Zheng Hua , Zhu Xiaohua 
Published: January 2025

AbstractIn this study, for the first time, we found a Subsurface Low Potential Vorticity Water (SLPVW) to the east of Taiwan Island (122–124, 21.67–23) via an array comprising 12 current and pressure‐recording inverted echo sounders (CPIESs) from 25 June 2018 to 29 July 2019. This SLPVW exhibits remarkable intraseasonal variability, with an ∼100‐day period east of Taiwan Island, corresponding with the variability in mesoscale eddies. Compared with large‐scale climatological mean circulation, mesoscale eddy can trap SLPVW as a highway westward transport. In addition, the SLPVW impingement results in a significant subsurface velocity variation (about 10 cm/s) in the 200–400 m layer. These findings shed new light on the mechanisms of the intraseasonal variation in the Kuroshio subsurface layer.
Stochastic Multi‐Observables Inversion for the 3D Thermochemical Structure of Lithosphere in Spherical Coordinates: Theory and Synthetic Examinations
Authors: Zhang Yi , Xu Yixian 
Published: January 2025

AbstractThe physical properties of the lithospheric and upper mantle's rock are determined by its composition and the in situ temperature and pressure conditions. Together, they have been referred to as the thermochemical structure. Information about the upper mantle's thermochemical structure could be obtained using methods from different disciplines of the earth sciences, in which the geophysical approaches show potential to map the 3D variations on both the regional and global scales. Thus, techniques for investigating the thermochemical structure in the spherical coordinates are needed, including forward modeling of the geophysical observables, calculating schemes of the thermophysical properties for the lithologies, and effective inversion algorithm, which is particularly important for large‐scale applications. This paper first demonstrates an adaptive meshing architecture based on the tetrahedral mesh by the sophisticated constructions in a spherical shell. Techniques that enable rapid calculations of the thermophysical properties of the upper mantle's rocks are introduced in length. Methodologies for constructing 3D thermochemical models and forward modeling geophysical observations, including an inversion sub‐routine that couples the lithostatic pressure and density variations to forward modeling, are introduced and examined in detail using synthetic data sets. We then introduce methods for determining 3D thermochemical structures of the upper mantle. The inverse problem is treated as a multi‐task evaluation process and solved using advanced stochastic optimizing techniques. Estimated uncertainties of the resultant thermochemical models are obtained simultaneously for error analysis. The proposed forward modeling and inversion techniques are validated using synthetic data sets in both forward and inversion circumstances. Limitations and further developments are discussed in the subsequent concluding remarks.
Activators of the 26S proteasome when protein degradation increases
Authors: Lee Donghoon 
Published: January 2025

Abstract
In response to extra- and intracellular stimuli that constantly challenge and disturb the proteome, cells rapidly change their proteolytic capacity to maintain proteostasis. Failure of such efforts often becomes a major cause of diseases or is associated with exacerbation. Increase in protein breakdown occurs at multiple steps in the ubiquitin-proteasome system, and the regulation of ubiquitination has been extensively studied. However, the activities of the 26S proteasome are also stimulated, especially under highly catabolic conditions such as those associated with atrophying skeletal muscle, proteotoxic stress such as heat shock and arsenite, or hormonal cues such as cAMP or cGMP agonists. Among the proteins that enhance proteasomal degradation are the PKA, PKG, UBL-UBA proteins and the Zn finger AN1-type domain (ZFAND) family proteins. ZFAND proteins are of particular interest because of their inducible expression in response to various stimuli and their abilities to control protein quality by stimulating the 26S proteasome and p97/VCP. The regulatory roles of ZFAND proteins appear to be important not only for the control of protein degradation but also for other cellular processes, such as mRNA stability and signaling pathways. This review summarizes the known functions of proteasome activators and discusses their possible roles in regulating proteostasis and other cellular processes.
Maternal effects in the model system Daphnia: the ecological past meets the epigenetic future
Authors: Agrelius Trenton C. , Dudycha Jeffry L. 
Published: January 2025

Abstract
Maternal effects have been shown to play influential roles in many evolutionary and ecological processes. However, understanding how environmental stimuli induce within-generation responses that transverse across generations remains elusive, particularly when attempting to segregate confounding effects from offspring genotypes. This review synthesizes literature regarding resource- and predation-driven maternal effects in the model system Daphnia, detailing how the maternal generation responds to the environmental stimuli and the maternal effects seen in the offspring generation(s). Our goal is to demonstrate the value of Daphnia as a model system by showing how general principles of maternal effects emerge from studies on this system. By integrating the results across different types of biotic drivers of maternal effects, we identified broadly applicable shared characteristics: 1. Many, but not all, maternal effects involve offspring size, influencing resistance to starvation, infection, predation, and toxins. 2. Maternal effects manifest more strongly when the offspring’s environment is poor. 3. Strong within-generation responses are typically associated with strong across-generation responses. 4. The timing of the maternal stress matters and can raise or lower the magnitude of the effect on the offspring’s phenotype. 5. Embryonic exposure effects could be mistaken for maternal effects. We outline questions to prioritize for future research and discuss the possibilities for integration of ecologically relevant studies of maternal effects in natural populations with the molecular mechanisms that make them possible, specifically by addressing genetic variation and incorporating information on epigenetics. These small crustaceans can unravel how and why non-genetic information gets passed to future generations.
Structural basis for the transport and regulation mechanism of the multidrug resistance-associated protein 2
Authors: Koide Eriko , Pietz Harlan L. , Beltran Jean , Chen Jue 
Published: January 2025

AbstractMultidrug resistance-associated protein 2 (MRP2) is an ATP-powered exporter important for maintaining liver homeostasis and a potential contributor to chemotherapeutic resistance. Using cryogenic electron microscopy (cryo-EM), we determine the structures of human MRP2 in three conformational states: an autoinhibited state, a substrate-bound pre-translocation state, and an ATP-bound post-translocation state. In the autoinhibited state, the cytosolic regulatory (R) domain plugs into the transmembrane substrate-binding site and extends into the cytosol to form a composite ATP-binding site at the surface of nucleotide-binding domain 2. Substrate displaces the R domain, permitting conformational changes necessary for transport. These observations suggest that the R domain functions as a selectivity gauge, where only at sufficiently high concentrations can the substrate effectively initiate transport. Comparative structural analyzes of MRP2 bound to various substrates, as determined in this study and others, reveal how MRP2 recognizes a diverse array of compounds, supporting its role in multidrug resistance.
New Silurian aculiferan fossils reveal complex early history of Mollusca
Authors: Sutton Mark D. , Sigwart Julia D. , Briggs Derek E. G. , Gueriau Pierre , King Andrew , Siveter David J. , Siveter Derek J. 
Published: January 2025

Abstract
Mollusca is the second most species-rich animal phylum, but the pathways of early molluscan evolution have long been controversial1–5. Modern faunas retain only a fraction of the past forms in this hyperdiverse and long-lived group. Recent analyses6–8 have consistently recovered a fundamental split into two sister clades, Conchifera (including gastropods, bivalves and cephalopods) and Aculifera9, comprising Polyplacophora (‘chitons’) and Aplacophora. Molluscan evolution in toto is characterized by plasticity in body-plan characters10, but historically aculiferans have been interpreted as more conservative10,11. The few completely preserved aculiferan or aculiferan-like fossils from the early Palaeozoic12–19 have been largely regarded as transitional forms that inform questions of character polarity between the extant polyplacophoran and aplacophoran body forms20,21. The history of early aculiferans, and the morphological and ecological range that they occupied, remain inadequately sampled. Here we describe two new three-dimensionally preserved aculiferan species from the Silurian Herefordshire Lagerstätte22,23, which substantially extend the morphological and ecological range of the clade. Phylogenetic analyses indicate positions within a complex nexus of taxa and suggest reversals in the states of fundamental characters such as the presence of valves and the nature of the foot. In contrast to previous hypotheses of morphological conservatism, evolution in early aculiferans generated a profusion of unusual forms comparable to the diversification of other crown-group molluscs.
Biochar filtration of drug-resistant bacteria and active pharmaceutical ingredients to combat antimicrobial resistance
Authors: Fady Paul-Enguerrand , Richardson Alexandra K. , Barron Leon P. , Mason A. James , Volpe Roberto , Barr Meredith R. 
Published: January 2025

AbstractAntimicrobial resistance (AMR) is a major cause of death worldwide, with 1.27 M direct deaths from bacterial drug-resistant infections as of 2019. Dissemination of multidrug-resistant (MDR) bacteria in the environment, in conjunction with pharmapollution by active pharmaceutical ingredients (APIs), create and foster an environmental reservoir of AMR. Creative solutions are required to mitigate environmental AMR, while taking into consideration other aspects of the planetary “Triple Crisis” of pollution, biodiversity loss, and climate change. Waste lignocellulosic biomass (LCB), a byproduct of agriculture and forestry, is the largest stream of non-edible biomass globally. Through pyrolysis, waste LCB can be converted into biochars, which have excellent attributes for adsorption of pollutants–though no studies have yet reliably correlated production conditions with efficacy, nor considered adsorption of human pathogens. By leveraging a bespoke pyrolysis reactor with precisely controlled parameters, we show that production conditions substantially affect sequestration of clinical bacterial isolates, removing up to 94% of Pseudomonas aeruginosa RP73 and 85% of Staphylococcus aureus EMRSA-15. In addition, we show that chars produced at higher peak pyrolysis temperatures (450 °C) can remove up to 88% of the antibiotic clarithromycin from wastewater, as well as significant proportions of many other APIs with varied physicochemical characteristics. These findings provide a first-in-kind insight into how production conditions affect the ability of biochars to mitigate environmental AMR.
Harnessing light-activated gallium porphyrins to combat intracellular Staphylococcus aureus using an in vitro keratinocyte infection model
Authors: Szymczak Klaudia , Rychłowski Michał , Zhang Lei , Nakonieczna Joanna 
Published: January 2025

AbstractStaphylococcus aureus (S. aureus) can survive inside nonprofessional phagocytes such as keratinocytes, enabling it to evade antibiotics and cause recurrent infections once treatment stops. New antibacterial strategies to eliminate intracellular, multidrug-resistant bacteria are needed. This study used a keratinocyte model infected with methicillin-resistant S. aureus (MRSA) to test light-activated compounds, specifically heme-mimetic gallium (III) porphyrin (Ga3+CHP) and visible light, known as antimicrobial photodynamic inactivation (aPDI), for eliminating intracellular MRSA. Ga3+CHP was found to accumulate more in infected cells, particularly within lysosomal structures where MRSA resides. Flow cytometry and fluorescence microscopy revealed significant colocalization of MRSA and Ga3+CHP. Under aPDI, MRSA showed reduced adhesion to host cells and a 70% reduction in the GFP signal from intracellular bacteria. Additionally, light-activated Ga3+CHP significantly decreased the number of extracellular bacteria, reducing the potential for further infection. This study is the first to analyze aPDI toxicity in real time within an infection model, demonstrating that this method is neither cytotoxic nor phototoxic.
Controllable synthesis of Pd and Pt shells on Au nanoparticles with electrodeposition
Authors: Elabbadi Mohsen , Boukouvala Christina , Ringe Emilie 
Published: January 2025

AbstractShells of Pd and Pt were synthesized on Au nanoparticles by electrodeposition, leading to controllable size and optical properties. This approach yielded core–shell structures with good homogeneity in size after the optimization of electrochemical parameters such as deposition current and charge transfer, as well as nanoparticle surface treatment. Dark field scattering microscopy and spectroscopy were used to track changes in the optical response of individual particles during deposition. The deposition of thin shells of Pd or Pt initially leads to a damping of the Au localized surface plasmon resonance which is followed by its redshift and an increase in scattering intensity. These changes were rationalized with numerical calculations and correlated with electron microscopy analyses revealing the morphology and thickness of Pd and Pt shells. This electrochemical approach provides a new pathway for the synthesis of bimetallic structures with catalytic surfaces.
Peony diagram modeling for improving the easy disassembly design of mobile electronic products
Authors: Huang Junling , Cui Chuangchuang , Jiang Wei , Zhu Libin 
Published: January 2025

AbstractDesigning mobile electronic products for easy disassembly is crucial for promoting resource recycling. However, many current approaches overlook the need to consider the disassembly of parts from the perspective of the overall product structure and practical recycling requirements, leading to potentially suboptimal or unnecessary optimization strategies. This study introduces a novel visual disassembly information model called the “Peony Diagram.” This model uses a multi-layer ring diagram to depict part hierarchies and constraint relationships and employs specific symbols to convey part disassembly information and highlight key components. The difficulty of disassembling individual parts is assessed by calculating difficulty coefficients based on quantified disassembly information in the model. To validate the model’s effectiveness, a smartphone was used as a case study to determine the shortest disassembly sequence and evaluate disassembly difficulty for both single and multiple parts. The findings demonstrate that the proposed method can accurately assess the ease of disassembly of key components during product repair and recycling. Consequently, it can suggest strategies to enhance recycling and disassembly efficiency and reduce the difficulty of disassembling critical parts.
A perfusion-independent high-throughput method to isolate liver sinusoidal endothelial cells
Authors: Babin-Ebell Gonçalves Anna , Mao Yifang , Baljkas Tinja , Wiedmann Felix , Eis Larissa , Pilz Franziska , Winkler Manuel , Kürschner-Zacharias Sina W. , Hoffarth Marlene , Funaya Charlotta , Shahidi Réza , Géraud Cyrill , Wu Chi-Chung , Schmidt Constanze , Goerdt Sergij , Reiners-Koch Philipp-Sebastian , Singhal Mahak 
Published: January 2025

AbstractLiver sinusoidal endothelial cells (LSECs) critically regulate homeostatic liver function and liver pathogenesis. However, the isolation of LSECs remains a major technological bottleneck in studying molecular mechanisms governing LSEC functions. Current techniques to isolate LSECs, relying on perfusion-dependent liver digestion, are cumbersome with limited throughput. We here describe a perfusion-independent high-throughput procedure to isolate LSECs with high purity. Indifferently from previous perfusion-independent approaches, chopped liver tissue was incubated in the digestion mix for 30 minutes with intermittent mixing with a serological pipette. This led to the safeguarding of LSEC integrity and yielded 10 ± 1.0 million LSECs per adult mouse liver, which is far higher than previous perfusion-independent protocols and comparable yield to established perfusion-dependent protocols for isolating LSECs. Combining magnetic and fluorescence-activated cell sorting (FACS), LSECs from different zones of the hepatic sinusoid can now be isolated in high numbers in less than two hours for downstream applications including proteomics. Our protocol enables the isolation of LSECs from fibrotic liver tissues from mice and healthy liver tissues from higher vertebrate species (pigs), where traditional perfusion-based digestion protocols have very limited application. In conclusion, these technical advancements reduce post-mortem changes in the LSEC state and aid in reliable investigation of LSEC functions.