bluuebunny/crossref_metadata_2025_split huggingface dataset:
Estimating the Cure Proportion in Stage IA Lung Adenocarcinoma: A population-based study
Authors: Huang Zhixin , Chen Dinghang , Hong Zhinuan , Kang Mingqiang 
Published: August 2023

Abstract

Objectives Using a mixed cure model, this study aimed to investigate factors influencing the cure, recurrence, and metastasis of stage IA lung adenocarcinoma. Materials and Methods A total of 1,064 patients who underwent video-assisted thoracoscopic pneumonectomy were included. Variable screening was performed using the random forest algorithm and least absolute shrinkage and selection operator. The mixed cure model was used to identify factors affecting patient cure and survival, and a sequential analysis was performed on 5%, 10%, and 20% of the presentational subtype simultaneously. The receiver operating characteristic was used to explore the best model and to construct a nomogram for predicting cure rate. Results The median follow-up was 58 (range: 3–115) months. Results from the cure part of the mixed model indicated that the predominant subtype, presentational subtype, and tumor diameter were prognostic factors affecting the patients' cure rate. Based on these factors, a nomogram was constructed to predict the cure rate. The survival part indicated that the predominant subtype was the only factor influencing patients' recurrence and metastasis. A sequential analysis of the presentational subtype showed no significant effect on survival (P > 0.05). Regardless of the recording mode, no significant improvement was observed in the model's discriminative ability. Only a few postoperative pathological specimens showed lymphovascular invasion (LVI); however, the survival curve suggested a significant effect on patient survival. Conclusion After excluding the existence of long-term survivors, the predominant subtype was the only factor influencing patient recurrence and metastasis. Although LVI is rare in stage IA, its significance for patient prognosis cannot be ignored.
Strategies of virginiamycin supplementation in the postweaning phase on growth performance and carcass quality of beef cattle
Authors: Gomes Rodrigo da Costa , Menezes Gilberto Romeiro de Oliveira , Favero Ricardo , Kazama Ricardo , Altrak Georg , Mizubuti Ivone Yurika , Bonin Marina de Nadai , Feijó Gelson Luis Dias , Montagner Denise Baptaglin , de Araújo Thiago Luis Alves Campos , Junior Dorgival Morais de Lima , Junior Roberto Augusto de Almeida Torres , Bonin Marcio de Nadai 
Published: August 2023

Abstract
The present study evaluated the effects of supplementing VM in grazing cattle during the rearing phase on performance and carcass quality of beef cattle in the finishing phase. Two experiments with a randomized block design were conducted in consecutive years to contrast two post-weaning supplementation strategies using VM at 45 mg/100 kg body weight (BW). In the first year, treatments were protein supplement in the dry season and mineral supplement in the rainy season versus the addition of VM both in the protein and mineral supplements. In the second year, was contrasted with protein supplement in the dry season and protein-energy supplement in the rainy season. Performance, carcass traits, and carcass quality were evaluated at the end of both phases. In Year 1, adding VM in mineral supplement increased final backfat thickness (P=0.05), backfat gain (P=0.06), final rump fat thickness (P=0.02), and rump fat gain (P=0.01). In the finishing phase, VM-treated cattle had a greater dry matter intake (P=0.03) and tended to show a greater backfat thickness than non-treated cattle (P=0.07). In Year 2, no VM effects were observed on post-weaning phase performance and carcass traits. However, cattle-fed VM during the post-weaning phase tended to show a lower feed conversion ratio (P=0.09) and had a significantly higher gross feed efficiency (P=0.03) than non-treated cattle at slaughter. Virginiamycin supplementation during rearing on pasture improves performance and carcass fattening in the growth phase and has a residual effect in the finishing phase that may reflect greater backfat thickness and gross feed efficiency.
Silica nanoparticle: Eco-friendly Waste Having Potential for Seed Germination of Wheat (Triticum turgidumL. Var. Sham) Under Salt Stress Conditions
Authors: Tabbal J.A. Al , Al-harahsheh Mohammad , Al-Zou’by Jehad , Al‑Zboon Kamel , Alrawashdeh Khalideh Al Bkoor 
Published: August 2023

Abstract

The germination, development, and production of directly seeded agricultural products may be impacted by a variety of abiotic stresses. Silica nanoparticle seed priming may be able to reduce these external stressors. Stress brought on by salt has now become a barrier to wheat (Triticum turgidum) farming success. The goal of the current research was to clarify the effectiveness of seed priming with silica nanoparticles in reducing salt-induced stress in wheat plants. In this research, silica nanoparticle at three distinct concentrations—300, 600 and 900 ppm—was used for seed priming either alone or in combination with sodium chloride (2.3 and 4.6 ds/m). The application of silica nanoparticles considerably improved seedling development while salinity stress greatly decreased germination percent and seedling growth. Seed priming significantly increased shoot length (11.53%) , root length (22.76%) , seedling length (17.57%) , shoot weight (35.56%) , root weight (19.14%) , germination stress tolerance index (91.55%), shoot length stress tolerance index (11.58%), root length stress tolerance index (22.6%), shoot weight stress tolerance index (35.54%) and root weight stress tolerance index (18.12%) under high saline treatment (4.6 ds/m). The significance of nano-silica in reducing the detrimental effects of salt stress on wheat seed growth is highlighted by the current investigation. The results showed that silica nanoparticle seed priming could enhance seedling early development under salt stress.
PM2.5 juvenile exposure-induced spermatogenesis dysfunction by triggering ferroptosis in adult male rats
Authors: Liu Xiang , Ai Yaya , Xiao Mingchen , Wang Cao , Shu Zhen , Yin Jia , Chu Yu , Xiao Qing , Liu Bin 
Published: August 2023

Abstract

PM2.5 derived from automobile exhaust can cause reproductive impairment in adult males, but the toxic effects of PM2.5 exposure on reproductive function in juvenile male rats and its relationship with ferroptosis have not been reported. In this paper, 30-day-old juvenile male Sprague-Dawley (SD) rats were divided into four groups (blank control, vitamin control , PM2.5, and PM2.5 + vitamin). The blank control group was fed normally, and the vitamin control group was given intragastric administration of vitamins in addition to normal feeding. PM2.5 was administered via trachea intubation. When the rats were treated for four weeks until reaching the period of sexual maturity. mating test were performed first, and then study of testicular and epididymal tissue in them. Compared with control rats, juvenile male rats exposed to PM2.5 showed a decreased sperm count and fertility rate, redox imbalance, damaged mitochondria, a metabolic disorder of intracellular iron ions, and a significant rise in ferroptosis during the period of sexual maturity. After vitamin intervention, the redox imbalance, metabolic disorder of intracellular iron ions, and ferroptosis were all alleviated, leading to the following conclusions: after being exposed to PM2.5 from automobile exhaust, male juvenile rats during the period of sexual maturity have significantly decreased reproductive function. The reproductive toxicity of PM2.5 is closely related to oxidative stress and ferroptosis. In addition, ferroptosis decreases and reproductive function is recovered to some degree after vitamin intervention.
Biopolymer-assisted enzyme-induced carbonate precipitation for Cu-contaminated loess remediation
Authors: Xie Yi-Xin , Cheng Wen-Chieh , Wang Lin , Xue Zhong-Fei , Xu Yin-Long 
Published: August 2023

Abstract

Wastewater, discharged in copper (Cu) mining and smelting, usually contains a large amount of Cu2+. Immobilizing Cu2+ in aqueous solution and soils is deemed crucial in preventing its migration into surrounding environments. In recent years, the enzyme-induced carbonate precipitation (EICP) has been widely applied to Cu immobilization. However, the effect of Cu2+ toxicity denatures and even inactivates the urease. In the present work, the biopolymer-assisted EICP technology was proposed. The inherent mechanism affecting Cu immobilization was explored through a series of test tube experiments and soil column tests. Results indicated that 4 g/L chitosan may not correspond to a higher immobilization efficiency because it depends as well on surrounding pH conditions. The use of Ca2+ not only prevented the nucleation of the urease with Cu2+ by the electrostatic repulsion but reduced the potential for Cu2+ to migrate into nearby environments when malachite and azurite minerals are wrapped by calcite minerals. The species of carbonate precipitation that are recognized in the numerical simulation supported the above claim. On the other hand, UC1 (urease and chitosan colloid) and UC2 (urea and calcium source) grouting reduced the effect of Cu2+ toxicity by transforming the exchangeable state-Cu into the carbonate combination state-Cu. The side effect, induced by 4 g/L chitosan, promoted the copper-ammonia complex formation in the shallow ground, while the acidic environments in the deep ground prevented Cu2+ from coordinating with soil minerals. These badly degraded the immobilization efficiency. The Raman spectroscopy and XRD test results tallied with the above results. The findings shed light on the potential of applying the biopolymer-assisted EICP technology to Cu-contaminated water bodies and sites remediation.
Identification and validation of TFs and lncRNAs in response to Tomato Leaf Curl Bangalore Virus (ToLCBaV) infection in tomato (Solanum spp.)
Authors: Chidambara Bhavya , Sadashiva Avverahally T. , M Krishna Reddy , Ravishankar Kundapura 
Published: August 2023

Abstract
Tomato leaf curl Bangalore virus (ToLCBaV), often a woeful reason for complete failure of tomato crop in southern India. The plant response to virus infection is a complicated network involving several defence routes. Every defence route involves transcription factors (TFs) and other regulatory transcripts including lncRNA as important regulators of gene expression. These regulators act as both pro-viral and anti-viral factors involved in modulating disease symptoms. This study has reported and validated several TFs and lncRNAs from transcriptome study of tomato lines, IIHR2611 (R) and IIHR 2843 (S) in response to virus infection. Validation was done based on expression analysis of selected TFs and lncRNAs using qPCR at different intervals of ToLCBaV infection. TFs, DREB protein 1A and Protein RADIALIS-like 3 were induced upon ToLCBaV infection. Whereas, WRKY TF-53 was upregulated in S and down regulated in R plants upon virus infection. Four lncRNAs were found differentially expressed among R and S lines when analysed using qPCR. The differential temporal gene expression of TFs among S and R plants in response to ToLCBaV infection regulate the resistance or susceptibility. Further studies on the mechanism of resistance of TFs and lncRNAs will aid in unravelling novel antiviral defence pathways in tomato.
Chitosan oligosaccharide suppresses osteosarcoma malignancy by inhibiting CEMIP via the PI3K/AKT/mTOR pathway
Authors: Sim IlJin , Choe WonGyom , Ri JinJu , Su Hang , Moqbel Safwat Adel Abdo , Yan Weiqi 
Published: August 2023

Abstract

Osteosarcoma is a malignant bone tumor that is prone to metastasize early and primarily affects children and adolescents. Cell migration-inducing protein (CEMIP) plays a crucial role in the progression and malignancy of various tumor diseases, including osteosarcoma. Chitosan oligosaccharide (COS), an oligomer isolated from chitin, has been found to have significant anti-tumor activity in various cancers. This study investigates the effects of COS on CEMIP expression in osteosarcoma and explores the underlying mechanism. In present study, in vitro experiments were conducted to confirm the inhibitory activity of COS on human osteosarcoma cells. Our results demonstrate that COS possesses inhibitory effects against human osteosarcoma cells and significantly suppresses CEMIP expression in vitro. Next, we studied the inhibition of the expression of CEMIP by COS and then performed bioinformatics analysis to explore the potential inhibitory mechanism of COS against signaling pathways involved in regulating CEMIP expression. Bioinformatics analysis predicted a close association between the PI3K signaling pathway and CEMIP expression and that the inhibitory effect of COS on CEMIP expression may be related to PI3K signaling pathway regulation. The results of this study show that COS treatment significantly inhibits CEMIP expression and the PI3K/AKT/mTOR signaling pathway, as observed both in vitro and in vivo. This study demonstrates that COS could inhibit the expression of CEMIP, which is closely related to osteosarcoma malignancy. This inhibitory effect may be attributed to the inhibition of the PI3K/AKT/mTOR signaling pathway in vitro and in vivo.
The potential crosstalk genes and molecular mechanisms between glioblastoma and periodontitis
Authors: Huang Jian-huang , Chen Yao , Kang Yuan-bao , Yao Zheng-jian , Song Jian-hua 
Published: August 2023

Abstract
Clinical and epidemiological evidence suggests a link between glioblastoma (GBM) and periodontitis (PD). However, the mechanisms of gene regulation shared by both are unclear. In this study, differentially expressed genes (DEGs) that overlap between the Gene Expression Omnibus (GEO) datasets GSE4290 [GBM] and GSE10334 [PD] were identified and functional enrichment analysis was performed. The protein-protein interaction (PPI) network, weighted gene co-expression network analysis (WGCNA) was performed to identified key modules. Then, we validated the key crosstalk genes in two independent external datasets. To further evaluate the correlation of key crosstalk genes with immune cells, the immune cell landscape of GBM and IAs was assessed. Finally, transcription factors (TFs) regulating key crosstalk genes were explored and their expression levels were further validated in the full dataset. We identified CXCR4, LY96 and C3 as key crosstalk genes, the expression levels of them were significantly elevated in the test dataset and external validation dataset. In addition, the expression of multiple immune cells was significantly elevated in GBM and PD compared to controls, and all three key crosstalk genes were significantly negatively associated with Macrophages M2. Finally, FLI1 was identified as a potential key TF, which regulates 3 key crosstalk genes and is upregulated in expression in the full dataset. The present study identified key crosstalk genes (CXCR4, LY96 and C3) and TF (FLI1) in GBM and PD from an immune and inflammatory perspective, providing new ideas for the comorbidity mechanism of GBM and PD.
Utilizing Visual Symptoms to Distinguish Dry Eye from Glaucoma, Cataract, and Suspect Glaucoma Patients: A Cross-Sectional Study
Authors: Zhao David , Quintero Michael , Mihailovic Aleksandra , Akpek Esen , Karakus Sezen , Guo Lee , Ramulu Pradeep 
Published: August 2023

Abstract
Background: The diagnosis of dry eye and other common ophthalmological conditions can be supported using patient reported symptoms, which is increasingly useful in contexts such as telemedicine. We aim to ascertain visual symptoms that differentiate dry eye from cataract, glaucoma, or glaucoma suspects.
Methods: Adults with dry eye, glaucoma, cataract, and suspected glaucoma (controls) completed a questionnaire to rate the frequency and severity of 28 visual symptoms. Univariate, followed by multivariable logistic regression with backward stepwise selection (p<0.05), determined the individual symptoms and set of symptoms best distinguishing dry eye from each of the other conditions.
Results: Mean age of 353 patients (94 glaucoma suspect controls, 79 glaucoma, 84 cataract, and 96 dry eye) was 64.1 years (SD=14.1); 67% were female and 68% White. Dry eye patients reported more frequent light sensitivity (OR=15.0, 95% CI=6.3-35.7) and spots in vision (OR=2.8, 95% CI=1.2-6.3) compared to glaucoma suspect controls. Compared to glaucoma patients, dry eye patients experienced more frequent light sensitivity (OR=9.2, 95% CI=2.0-41.7), but less frequent poor peripheral vision (OR=0.2, 95% CI=0.06-0.7), difference in vision between eyes (OR=0.09, 95% CI=0.01-0.7), and missing patches of vision (OR=0.06, 95% CI=0.009-0.3). Compared to cataract patients, dry eye patients reported more frequent spots in vision (OR=4.5, 95% CI=1.5-13.4) and vision variability across the week (OR=4.7, 95% CI=1.2-17.7) and were less likely to report worsening vision (OR=0.1, 95% CI=0.03-0.4) and blindness (OR=0.1, 95% CI=0.02-0.8).
Conclusion: Dry eye can be distinguished from various ocular conditions using visual symptoms, though the symptoms that best distinguish dry eye differ across comparisons. Differentiating how patients visually perceive common eye diseases may be used in a variety of clinical settings to rule out specific conditions.
Use of structured systems as a strategy to minimize the deactivation of Ni-based catalysts applied in dry reforming of methane
Authors: de Araújo Ítalo R. S. , Ribeiro André T.S. , Araújo I. C. F. , Araújo N. B. S. , Sanz Oihane , Garcia Ramón R. P. , Sales Deivson C. S. , Almeida Luciano 
Published: August 2023

Abstract
The main challenge in the use of Ni based catalysts is the high deactivation rate of these catalysts. In this work, strategies aimed at improving this characteristic such as the use of structured catalysts were studied. In this work, the Ni/γ-Al2O3 (Ni/Al) and Ni/La2O3-γ-Al2O3 (Ni/La-Al) catalysts were synthesized by the all-in-one method and used in the Dry Reforming of Methane combined with its application in structured systems to minimize the effects of deactivation. The catalysts were characterized and a smaller Ni crystallite size for the La-promoted catalyst was observed. The deactivation of the structured catalysts and application of residual activity deactivation models (DMRA) were evaluated by applying different weight hourly velocities (WHSV). Besides that, the regeneration of the catalysts was developed through the comparison of the treatment with CO2 and H2 atmospheres. Furthermore, the greatest and the lowest deactivation of the structured systems were identified for the WHSV values of 40 and 20 L gcat−1 h− 1, respectively. Finally, the regeneration treatment with CO2 showed to be more efficient than the treatment with H2. A deactivation model was predicted in the region of equilibrium in the catalytic activity, which is associated with the appearance of a residual activity, which decreases with increasing WHSV variable.
Experimental study on effects of air injection on cavitation pressure pulsation and vibration in a centrifugal pump with inducer
Authors: Mao Yanhong , Liu Houlin , Wang Yong , Chen Jie , Liu Fei 
Published: August 2023

AbstractCavitation commonly induces performance deterioration and system vibration in many engineering applications. This paper aims to investigate the effects of air injection on cavitation evolution, pressure pulsation and vibration in a centrifugal pump with inducer. In this paper, the high-speed camera is used to capture the gas flow pattern and cavitation evolution process in the inducer. The impacts of air injection on the inlet pressure pulsation and vibration are also investigated. The results show that the cavitation development in the inducer undergoes four patterns: incipient cavitation, sheet cavitation, cloud cavitation and super cavitation. As the cavitation becomes more severe, the main frequency of the pressure pulsation shifts to lower frequencies, and the amplitude of the vibration increase. In addition, air injection promotes the incipient cavitation but delays the cavitation development. A small amount of air makes cavity shed with small-scale clusters. Meanwhile, it can effectively decrease amplitudes of pressure pulsation and vibration. As the air content increases, the fluctuations and amplitudes of pressure pulsation and vibration increase.
Phyto-synthesis of Copper Nanoparticles and Its Application in Medical Fabrics and Evaluation of Antibacterial Activity
Authors: Janakiraman Vardhana , G Reethiga , B Sampathkumar , N.K Udayaprakash 
Published: August 2023

Abstract

Background: Copper's potent antimicrobial properties make it essential in medical fabrics, enhancing infection control and cleanliness. Phyto-synthesis, using plant-based materials for nanoparticle synthesis, offers an eco-friendly approach that harnesses the natural antimicrobial properties of plants. Curcuma aromatica, rich in bioactive compounds, shows potential for synthesizing antimicrobial copper nanoparticles (CuNPs).
Methods: CuNPs were synthesized using Curcuma aromatica extract and characterized using UV-Vis spectroscopy, Scanning Electron Microscope (SEM), Fourier transform infrared (FTIR) analysis, and X-ray diffraction (XRD). The antibacterial efficacy of the synthesized CuNPs was evaluated against Staphylococcus aureus and Escherichia coli using the well diffusion method. The CuNPs embedded cotton fabrics were also evaluated for their antimicrobial activity.
Results: The UV-VIS Spectroscopy showed absorption peaks above 495 nm due to the plasmon resonance of CuNPs. The synthesized nanoparticles ranged in size from 54 to 69 nm which was confirmed by SEM images. FTIR analysis indicated the presence of various organic molecules associated with CuNPs. XRD analysis revealed the face-centered cubic crystal structure of the CuNPs. The CuNPs exhibited significant antibacterial activity against both Staphylococcus aureus and Escherichia coli, as evidenced by the inhibition zones. The CuNPs embedded cotton fabrics demonstrated antimicrobial activity against the tested pathogens.
Conclusion:In this study, CuNPs were synthesized using Curcuma aromatica extract and exhibited antimicrobial activity against Staphylococcus aureus and Escherichia coli. The incorporation of CuNPs into cotton fabrics resulted in sustained antimicrobial activity, indicating their potential for antimicrobial textile development. These findings support the use of Curcuma aromatica as a suitable source for CuNP synthesis and emphasize the significance of CuNPs in the development of effective antimicrobial fabrics for medical applications.