bluuebunny/crossref_metadata_2025_split huggingface dataset:
Dot‐ELISA based on recombinant Hypodermin C of Przhevalskiana silenus for field diagnosis of goat warble fly infestation
Authors: Yadav Vikas , Rafiqi Shafiya Imtiaz , Yadav Anish , Kushwaha Anand , Godara Rajesh , Katoch Rajesh , Panadero‐Fontán Rosario 
Published: August 2023

AbstractGoat warble fly infestation (GWFI) is an economically important myiasis caused by larvae of Przhevalskiana silenus (Diptera, Oestridae), prevalent in countries of the Mediterranean Basin and Indian subcontinent. GWFI is characterized by the presence of subcutaneous warbles at the lumbar and sacral region of dorsum in the infested animal. The early larval instars (L1 and L2) remain inaccessible to physical detection due to their small size and subcutaneous presence thus causing hindrance in the diagnosis. The objective of present study was to develop a field applicable early diagnostic intervention for GWFI monitoring and prophylactic management for effective control of the disease. Recombinant Hypodermin C (rHyC) antigen of P. silenus was expressed in Escherichia coli. The purified protein was used for optimizing dot‐ELISA in a checkerboard titration using goat warble fly infested serum as known positive. The optimized assay was further tested for lower temperature (18°C) and incubation time (30 min). The optimized assay was assessed for inter‐rater reliability and field samples. The optimized conditions require 188 ng of protein/dot, 1:800 dilution of serum sample, 1:4000 dilution of anti‐goat IgG conjugate and 5% skim milk powder in phosphate buffer saline as blocking buffer. The assay was found to have a diagnostic sensitivity and specificity of 97.3% and 95.8%, respectively. The inter‐rater reliability of dot ELISA with rHyC indirect ELISA was found to be almost perfect with a Cohen's kappa index of 0.973. Further testing at ambient temperature (18°C) and shorter incubation steps (30 min) supported suitability of the assay for field diagnosis of GWFI. The present study provides the first report of a sensitive and specific dot‐ELISA for early diagnosis of GWFI which is rapid and cost effective. The test may provide an effective field applicable tool for sustainable control of GWFI.
When you need to dive in the deep end—Transplanting SARS‐CoV‐2 PCR+ recipients
Authors: Zavala Sofia , DeLaurentis Clare , Aaron Justin G. , Miko Benjamin A. , Fox Alyson N. , Bergelson Michael , DeVore Adam , Segovia Maria Cristina , Wolfe Cameron R. , Pereira Marcus R. 
Published: August 2023

AbstractThere is an ongoing need to understand whether transplantation during acute Coronavirus disease 2019 (COVID‐19) can be performed safely, especially when urgent transplant is required. We collected retrospective data of all consecutive non‐lung transplant recipients who had a positive SARS‐CoV‐2 polymerase chain reaction (PCR) on the day of planned deceased donor organ implantation. Data were collected from two large transplant centers from 01/01/2022 to 02/01/2023. Demographics, details regarding COVID‐19 infection, waitlist priority, and details regarding transplantation were obtained. A descriptive analysis was performed. A total of 12 patients were identified: 7 renal, 4 liver, and 1 heart transplant recipient. All 12 patients were vaccinated for COVID‐19. Ten were asymptomatic outpatients found positive on admission and transplanted immediately. Two were in‐patients with mild COVID‐19 symptoms and were reactivated on the waitlist following 3 days of remdesivir when no progression to severe COVID‐19 occurred. Most patients (10/12) received remdesivir posttransplant. No complications attributed to COVID‐19 were noted nor were any secondary family or healthcare worker infections observed. All recipients were managed with special isolation precautions befitting their potentially infectious state. Standard induction therapy was used in all recipients. After a median follow up period of 143 days (interquartile range: 96–201 days), 3 episodes of rejection were documented, 2/7 renal recipients experienced delayed graft function, and 2/4 liver recipients required renal replacement therapy. Graft and patient survival were 100%. Transplantation can safely proceed in select, minimally symptomatic, non‐lung recipients with a positive SARS‐CoV‐2 PCR at the time of transplant.
image
AXR1 modulates trichome morphogenesis through mediating ROP2 stability in Arabidopsis
Authors: Liu Lu , Niu Linyu , Ji Ke , Wang Yali , Zhang Chi , Pan Mi , Wang Wenjia , Schiefelbein John , Yu Fei , An Lijun 
Published: July 2023

SUMMARYCell differentiation and morphogenesis are crucial for the establishment of diverse cell types and organs in multicellular organisms. Trichome cells offer an excellent paradigm for dissecting the regulatory mechanisms of plant cell differentiation and morphogenesis due to their unique growth characteristics. Here, we report the isolation of an Arabidopsis mutant, aberrantly branched trichome 3–1 (abt3‐1), with a reduced trichome branching phenotype. Positional cloning and molecular complementation experiments confirmed that abt3‐1 is a new mutant allele of Auxin resistant 1 (AXR1), which encodes the N‐terminal half of ubiquitin‐activating enzyme E1 and functions in auxin signaling pathway. Meanwhile, we found that transgenic plants expressing constitutively active version of ROP2 (CA‐ROP2) caused a reduction of trichome branches, resembling that of abt3‐1. ROP2 is a member of Rho GTPase of plants (ROP) family, serving as versatile signaling switches involved in a range of cellular and developmental processes. Our genetic and biochemical analyses showed AXR1 genetically interacted with ROP2 and mediated ROP2 protein stability. The loss of AXR1 aggravated the trichome defects of CA‐ROP2 and induced the accumulation of steady‐state ROP2. Consistently, elevated AXR1 expression levels suppressed ROP2 expression and partially rescued trichome branching defects in CA‐ROP2 plants. Together, our results presented a new mutant allele of AXR1, uncovered the effects of AXR1 and ROP2 during trichome development, and revealed a pathway of ROP2‐mediated regulation of plant cell morphogenesis in Arabidopsis.
Comparison of three methods of tonometry in horses presented for ophthalmic disease
Authors: Latham Emily , Scherrer Nicole M. , Stefanovski Darko 
Published: August 2023

AbstractObjectiveTo compare the measurement of intraocular pressure in horses with clinical ocular disease using three tonometry devices.Animals StudiedA total of fifty horses were presented to the New Bolton Center Ophthalmology Service.ProcedureIntraocular pressure was taken on 50 client‐owned horses (100 eyes) using the TonoVet, TonoVet Plus and Tono‐Pen Avia tonometers. Clinical equine patients included were presented to New Bolton Center for ophthalmic examination. Auriculopalpebral blocks were performed but horses were unsedated.ResultsAll three tonometers were found to have strong agreement among them. The strongest agreement was between the TonoVet and the TonoVet Plus with the TonoVet on average 1.330 mmHg less than the TonoVet Plus (standard deviation 4.388 mmHg). This was followed by the TonoVet and the Tono‐Pen Avia Vet with the TonoVet on average 2.531 mmHg greater than the Tono‐Pen Avia Vet (standard deviation 4.124 mmHg). The weakest agreement was between the TonoVet Plus and the Tono‐Pen Avia with the TonoVet Plus on average 3.854 mmHg greater than the Tono‐Pen Avia (standard deviation 4.724 mmHg).ConclusionsAll three tonometers showed strong agreement, however, the TonoVet and the TonoVet Plus carried the strongest agreement with the TonoVet Plus having slightly higher measurements overall compared with the TonoVet. Due to small variations between devices, it is recommended that the same device be used for serial measurements of intraocular pressure. However, all three devices are appropriate to use in horses presented for ophthalmic evaluation.
A general approach to transversal versions of Dirac‐type theorems
Authors: Gupta Pranshu , Hamann Fabian , Müyesser Alp , Parczyk Olaf , Sgueglia Amedeo 
Published: July 2023

AbstractGiven a collection of hypergraphs with the same vertex set, an ‐edge graph is a transversal if there is a bijection such that for each . How large does the minimum degree of each need to be so that necessarily contains a copy of that is a transversal? Each in the collection could be the same hypergraph, hence the minimum degree of each needs to be large enough to ensure that . Since its general introduction by Joos and Kim (Bull. Lond. Math. Soc. 52 (2020) 498–504), a growing body of work has shown that in many cases this lower bound is tight. In this paper, we give a unified approach to this problem by providing a widely applicable sufficient condition for this lower bound to be asymptotically tight. This is general enough to recover many previous results in the area and obtain novel transversal variants of several classical Dirac‐type results for (powers of) Hamilton cycles. For example, we derive that any collection of graphs on an ‐vertex set, each with minimum degree at least , contains a transversal copy of the th power of a Hamilton cycle. This can be viewed as a rainbow version of the Pósa–Seymour conjecture.
A dilation theoretic approach to approximation by inner functions
Authors: Alpay Daniel , Bhattacharyya Tirthankar , Jindal Abhay , Kumar Poornendu 
Published: July 2023

AbstractUsing results from the theory of operators on a Hilbert space, we prove approximation results for matrix‐valued holomorphic functions on the unit disc and the unit bidisc. The essential tools are the theory of unitary dilation of a contraction and the realization formula for functions in the unit ball of . We first prove a generalization of a result of Carathéodory. This generalization has many applications. A uniform approximation result for matrix‐valued holomorphic functions which extend continuously to the unit circle is proved using the Potapov factorization. This generalizes a theorem due to Fisher. Approximation results are proved for matrix‐valued functions for whom a naturally associated kernel has finitely many negative squares. This uses the Krein–Langer factorization. Approximation results for ‐contractive meromorphic functions where induces an indefinite metric on are proved using the Potapov–Ginzburg theorem. Moreover, approximation results for holomorphic functions on the unit disc with values in certain other domains of interest are also proved.
Generalized Polish spaces at regular uncountable cardinals
Authors: Agostini Claudio , Motto Ros Luca , Schlicht Philipp 
Published: July 2023

AbstractIn the context of generalized descriptive set theory, we systematically compare and analyze various notions of Polish‐like spaces and standard ‐Borel spaces for an uncountable (regular) cardinal satisfying . As a result, we obtain a solid framework where one can develop the theory in full generality. We also provide natural characterizations of the generalized Cantor and Baire spaces. Some of the results obtained considerably extend previous work from Coskey and Schlicht (Fund. Math. 232 (2016) 227–248), Galeotti (Ph.D. thesis, Universität Hamburg, 2019), and Lücke and Schlicht (Israel J. Math. 209 (2015) 421–461), and answer some questions contained therein.
Ply-Blocking Phenomenon and Hole Size Effects in Modeling Progressive Damage in Fiber-Reinforced Plastics Laminates
Authors: Divse Vishwas , Joshi Suhas S. , Marla Deepak 
Published: August 2023

Abstract
This work presents a 3D progressive damage model based on Puck’s failure theory and linear damage evolution in fiber-reinforced plastic (FRP) laminates. It includes shear nonlinearity, in situ strengths, equivalent stress–strain, and mixed-mode fracture energy, and is implemented in abaqus/explicitTM through VUMAT subroutine. Various test cases were performed to validate the model and demonstrate its applications. The shear nonlinearity test shows that transverse compression retards matrix microcracking while transverse tension accelerates it. The open hole tension (OHT) test of laminates shows that delamination initiates around the holes and free edges, spreads the most, and propagates in different directions at different interfaces. Later, interfiber damage in 45 deg or −45 deg plies initiates and spreads at a slight inclination to the tip of the hole. Finally, fiber damage in 0 deg plies initiates at the tip of the hole, spreads the least, and propagates perpendicular to the loading direction. The ply-blocked laminates show around 30% higher strength and fracture strain than non-ply-blocked laminate due to delay in damage propagation, and are less sensitive to the hole size. Accordingly, their OHT strength reduces by 14.3% as opposed to 21.14% in the non-ply-blocked laminates, when the hole size increases from 6 to 9 mm. The damage location, magnitude, and propagation were corroborated with experimental findings in the literature.
Genome sequence of an indole-3-acetic acid and siderophores producing
Pseudomonas monsensis
SARCC-3054 with the potential as a microbial inoculant for enhanced crop production
Authors: Hassen Ahmed Idris , Khambani Langutani Sanger , Pierneef Rian 
Published: August 2023

ABSTRACT

The genome of
Pseudomonas monsensis
strain SARCC-3054 was sequenced after being confirmed as a potential plant growth-promoting rhizobacteria in both
in vitro
and
in vivo
assays. The 6.3 MB genome has a GC content of 60.2% and is divided into 59 contigs that contain several plant beneficial genes and proteins.
Carbon dioxide flushing versus saline flushing of thoracic aortic stents (INTERCEPTevar): protocol for a multicentre pilot randomised controlled trial
Authors: Crockett Stephen , Hanna Lydia , Singh Abhinav , Gunning Stephen , Nicholas Richard , Bicknell Colin , Hamady Mohamad , Gable Dennis , Sallam Morad , Modarai Bijan , Abisi Said , Lyons Oliver , Gibbs Richard 
Published: July 2023

IntroductionThoracic endovascular aortic repair (TEVAR) carries a 3%–6.1% stroke risk, including risk of ‘silent’ cerebral infarction (SCI). Stent-grafts are manufactured in room air and retain air. Instructions for use recommend saline flushing to ‘de-air’ the system prior to insertion, but substantial amounts of air are released when deploying them, potentially leading to downstream neuronal injury and SCI. Carbon dioxide (CO2) is more dense and more soluble in blood than air, without risk of bubble formation, so could be used in addition to saline to de-air stents. This pilot trial aims to assess the feasibility of a full-scale randomised controlled trial (RCT) investigating the neuroprotective benefit against SCI with the use of CO2-flushed aortic stent-grafts.Methods and analysisThis is a multicentre pilot RCT, which is taking place in vascular centres in the UK, USA and New Zealand. Patients identified for TEVAR will be enrolled after informed written consent. 120 participants will be randomised (1:1) to TEVAR-CO2or TEVAR-saline, stratified according to TEVAR landing zone. Participants will undergo preoperative neurocognitive tests and quality of life assessments, which will be repeated at 6 weeks, or first outpatient appointment, and 6 months. Inpatient neurological testing will be performed within 48 hours of return to level 1 care for clinical stroke or delirium. Diffusion-weighted MRI will be undertaken within 72 hours postoperatively (1–7 days) and at 6 months to look for evidence and persistence of SCI. Feasibility will be assessed via measures of recruitment and retention, informing the design of a full-scale trial.Ethics and disseminationThe study coordination centre has obtained approval from the London Fulham Research Ethics Committee (19/LO/0836) and Southern Health and Disability Ethics Committee (NZ) and UK’s Health Regulator Authority (HRA). The study has received ethical approval for recruitment in the UK (Fulham REC, 19/LO/0836), New Zealand (21/STH/192) and the USA (IRB 019-264, Ref 378630). Consent for entering into the study will be taken using standardised consent forms by the local study team, led by a local PI. The results of the trial will be submitted for publication in an open access journal.Trial registration numberNCT03886675
Efficacy of coenzyme Q10 supplementation for male infertility with high
sperm DNA fragmentation index: a protocol for a systematic review and
meta-analysis
Authors: Zhao Yu , Zhao Xiaoli , Zhang Guangyin , Ma Ruihong , Geng Qiang , Ouyang Bin , Xia Tian 
Published: July 2023

Introduction
Infertility is a focal issue in public health and affects human
reproduction and survival. Notably, an increasing number of studies in
recent decades have found that sperm DNA integrity plays a critical role
in the development of healthy embryos. Among the multiple pathogenic
factors of sperm DNA fragmentation, oxidative stress has proven to be
predominant. Coenzyme Q10 supplementation, which has been used for the
treatment of male infertility, has shown good clinical efficacy due to
its oxidation resistance, but its efficacy as measured by the sperm DNA
fragmentation index remains controversial. To address this issue, we
will perform a systematic review and meta-analysis to evaluate the
efficacy of coenzyme Q10 for male infertility patients with a high sperm
DNA fragmentation index.

Methods and analysis
The PubMed, Embase, Cochrane Central Register of Studies and Web of
Science databases will be comprehensively searched from inception to 31
December 2022 to identify relevant studies published in the English
language using appropriate search strategies. The search terms will be
derived from the following concepts: sperm DNA fragmentation, coenzyme
Q10 and randomised controlled trials. Two review stages, that is, title
and abstract screening and full-text screening, will be performed by two
reviewers. The risk of bias, publication bias and evidence grade of the
included studies will be assessed using a standardised protocol. Data
will be used to calculate effect sizes. Heterogeneity among the studies
will be evaluated graphically. Subgroup analysis and sensitivity
analysis will be performed if necessary to validate the
results.

Ethics and dissemination
No ethical approval will be needed, as there will be no participants
in this study. We will follow the Preferred Reporting Items for
Systematic Reviews and Meta-Analyses guidelines to disseminate the
findings through publication and conference presentation.

PROSPERO registration number
CRD42022293340.
Analysis of the key themes in the healthcare of older people with multimorbidity in Germany: a framework analysis as part of the LoChro trial
Authors: Salm Claudia , Mentzel Anja , Sofroniou Mario , Metzner Gloria , Farin Erik , Voigt-Radloff Sebastian , Maun Andy 
Published: August 2023

ObjectivesMultimorbidity challenges healthcare systems. In Germany, coordination of healthcare for older multimorbid patients remains unstructured.This study aims to identify key themes in the healthcare of these patients and the inter-relationships between them.DesignFramework analysis of six cases based on 1-year data of primary and secondary care, patient-answered questionnaires and video material.SettingSouthern Germany.ParticipantsSix multimorbid older patients participating in a randomised controlled trial that compared usual care with a local, collaborative, stepped and personalised care management approach for older people with chronic diseases (LoChro-trial). The LoChro care intervention involved a care manager who assisted participants in self-management. The primary outcome was a composite of functional health and depressive symptoms at 12 and 18 months. The LoChro-intervention had no effect on the primary outcome.Primary outcome measureKey themes in the healthcare of older patients with multimorbidity and the inter-relationships between them.MethodsOne-year data included diagnoses, treatment plans, examinations, assessments and discharge reports. Patient perspectives were assessed using the Patient Assessment of Chronic Illness Care. In three cases, videos of the LoChro intervention showed patients describing their health needs. These data were evaluated by three doctors and public health researchers. Using framework analysis, recurring themes influencing the healthcare situation of multimorbid older patients and their inter-relationships were identified.ResultsParticipants had an average age of 77, with 13 diagnoses, taking eight medications regularly. The five key themes describing the healthcare situation of these multimorbid patients were as follows:insufficient coordination,overuseandunderuse of medical care,doctorandpatient roles. Each theme covered three to four subcategories. The most significant inter-relationships between these themes were a lack of coordination leading to overuse and underuse of medical care. These were characterised by redundant inpatient stays, potential prescribing omissions and missed examinations. Deficiencies in vaccinations and secondary prevention were also demonstrated.ConclusionCoordination of care for multimorbid older patients in Germany is still deficient. Future healthcare arrangements should be explored with the participation of physicians and patients.Trial registration numberLoChro trial: DRKS00013904.
Effects of intensive lipid lowering compared with moderate-intensity
lipid lowering on coronary atherosclerotic plaque phenotype and major
adverse cardiovascular events in adults with low to intermediate 10-year
ASCVD risk (ILLUMINATION study): protocol for a multicentre, open-label,
blinded-endpoint, randomised controlled trial
Authors: Zheng Jianan , Hou Zhihui , Yuan Jinqing , Zhao Xueyan , Wang Yang , Li Jia , Zhang Wenjia , Dou Kefei , Lu Bin 
Published: July 2023

Introduction
Current guidelines recommend moderate-intensity lipid lowering
(low-density lipoprotein cholesterol, LDL-C of <2.6 mmol/L or 30%–49%
reduction from the baseline) for patients with intermediate 10-year
atherosclerotic cardiovascular disease (ASCVD) risk. The effects of
intensive lipid lowering (LDL-C of <1.8 mmol/L) on coronary
atherosclerotic plaque phenotype and major adverse cardiovascular events
(MACE) in adults with both non-obstructive coronary artery disease (CAD)
and low to intermediate 10-year ASCVD risk remain uncertain.

Methods and analysis
Intensive Lipid-lowering for Plaque and Major Adverse Cardiovascular
Events in Low to Intermediate 10-year ASCVD Risk Population is a
multicentre, randomised, open-label, blinded endpoint clinical trial.
Inclusion criteria are as follows: (1) patients with the age of 40–75
years within 1 month of coronary CT angiography (CCTA) and coronary
artery calcium score (CACS) evaluation; (2) population with low to
intermediate 10-year ASCVD risk (<20%) and (3) patients with
non-obstructive CAD (stenosis <50%) using CCTA. 2900 patients will be
randomly assigned to the intensive lipid lowering (LDL-C of
<1.8 mmol/L or ≥50% reduction from the baseline) or the
moderate-intensity lipid lowering (LDL-C of<2.6 mmol/L or 30%–49%
reduction from the baseline) group in a 1:1 ratio. The primary endpoint
is MACE (composite of all-cause death, non-fatal MI, non-fatal stroke,
any revascularisation and hospitalisation for angina) within 3 years
after enrolment. The secondary endpoints are changes in coronary total
plaque volume (mm3), plaque burden (%), plaque
composition (mm3, %), high-risk plaque
characteristics detected using CCTA and CACS determined using
CT.

Ethics and dissemination
Ethics committee approval for this study was obtained from the
review boards of Fuwai Hospital (No.2022-1787) and all other study
sites. Written informed consent will be obtained from all participants.
The results of this study will be published in peer-reviewed journals
and reported at international conferences.

Trial registration number
NCT05462262.