bluuebunny/crossref_metadata_2025_split huggingface dataset:
Multilayer Graphene Oxide‐Silver Nanoparticles for Stable, Highly Sensitive, and Reusable SERS Platforms
Authors: Thuy An Nguyen , Kieu Thi Ta Hanh , Van Hoang Dung , Phung Viet‐Duc , Hoa Thi Tran Nhu , Thang Phan Bach 
Published: January 2023

AbstractIntegrating noble metal nanoparticles and graphene materials to enhance the Raman signal is of great significance in surface‐enhanced Raman scattering which enhancement silver nanoparticles (Ag) which are reported one of the strongest plasmonic properties materials that are easily synthesized. Graphene and its derivatives such as reduced graphene oxide (rGO) are capable of increasing the charge transfer between the adsorbent and the substrates leading to a chemical mechanism. The combination of the rGO/Ag nanohybrids shows great potential as substrates which is the association of the electromagnetic and chemical mechanism for more enhancement of the Raman signal. We report a rapid, easy, and low‐cost method to fabricate rGO/Ag substrate for SERS using methylene blue as a probe molecule. The density of Ag This material exhibits superior sensitivity for detecting methylene blue down to a concentration of 10−9 M and enhancement factor SERS calculated reaches 108 demonstrations of the excellent Raman signal enhancement of rGO/Ag substrate. The relative standard deviation value calculated is 4.91% showing extremely good homogeneity and reproducibility, indicating the potential application in quantitative determination. The obtained results contribute to the development and optimization of substrates for application in the detection of trace amounts of organic substances in food and environmental applications.
“Liquid‐To‐Solid” Conversion of Biomass Wastes Enhanced by Uniform Nitrogen Doping for the Preparation of High‐Value‐Added Carbon Materials for Energy Storage with Superior Characteristics
Authors: Chernysheva Daria V. , Sidash Ekaterina A. , Konstantinov Maksim S. , Klushin Victor A. , Tokarev Denis V. , Andreeva Veronica E. , Kolesnikov Evgeny A. , Kaichev Vasily V. , Smirnova Nina V. , Ananikov Valentine P. 
Published: January 2023

AbstractSustainable human development urgently calls for decreasing the cost of energy storage. Continuous massive consumption of dedicated carbon electrode materials with complex internal molecular architectures requires rethinking both the source of materials and the process of their production. Finding an efficient sustainable solution is focused on the reuse and development of waste processing into corresponding high‐value‐added carbon materials. The processing of solid wastes into solid value‐added carbon materials (“solid‐to‐solid”) is relatively well developed but can be a two‐stage process involving carbon architecture rearrangement and heteroatom doping. Processing liquid wastes into high‐value‐added solid material (“liquid‐to‐solid”) is typically much more challenging with the need for different production equipment. In the present study, a new approach is developed to bypass the difficulty in the “liquid‐to‐solid” conversion and simultaneously built in the ability for heteroatom doping within one production stage. Polycondensation of liquid humins waste with melamine (as a nitrogen‐containing cross‐linking component) results in solidification with preferential C and N atomic arrangements. For subsequent thermochemical conversion of the obtained solidified wastes, complicated equipment is no longer required, and under simple process conditions, carbon materials for energy storage with superior characteristics were obtained. A complete sequence is reported in the present study, including liquid waste processing, nitrogen incorporation, carbon material production, structural study of the obtained materials, detailed electrochemical evaluation and real supercapacitor device manufacture and testing.
LncRNA BC promotes lung adenocarcinoma progression by modulating IMPAD1 alternative splicing
Authors: Chen Qi Wen , Cai Qian Qian , Yang Ying , Dong Shu , Liu Yuan Yuan , Chen Zhong Yi , Kang Chun Lan , Qi Bing , Dong Yi Wei , Wu Wei , Zhuang Li Ping , Shen Ye Hua , Meng Zhi Qiang , Wu Xing Zhong 
Published: January 2023

AbstractBackgroundThe therapeutic value of targeted therapies in patients with lung cancer is reduced when tumours acquire secondary resistance after an initial period of successful treatment. However, the molecular events behind the resistance to targeted therapies in lung cancer remain largely unknown.AimsTo discover the important role and mechanism of lncRNA BC in promoting tumor metastasis and influencing clinical prognosis of LUAD. Materials & Methods: Microarrays were used to screen a comprehensive set of lncRNAs with differential expression profiles in lung cancer cells. The functional role and mechanism of lncRNA were further investigated by gain‐ and loss‐of‐function assays. RNA pull‐down, protein assays, and mass spectrometry were used to identify proteins that interacted with lncRNA. TaqMan PCR was used to measure lncRNA in lung adenocarcinoma and adjacent nontumor tissues from 428 patients. The clinical significance of lncRNA identified was statistically confirmed in this cohort of patients.ResultsIn this study, we show that the long non‐coding RNA BC009639 (BC) is involved in acquired resistance to EGFR‐targeted therapies. Among the 235 long non‐coding RNAs that were differentially expressed in lung cancer cell lines, with different metastatic potentials, BC promoted growth, invasion, metastasis, and resistance to EGFR‐tyrosine kinase inhibitors (EGFR‐TKIs), both in vitro and in vivo. BC was highly expressed in 428 patients with lung adenocarcinoma (LUAD) and high BC expression correlated with reduced efficacy of EGFR‐TKI therapy. To uncover the molecular mechanism of BC‐mediated EGFR‐TKI resistance in lung cancer, we screened and identified nucleolin and hnRNPK that interact with BC. BC formed the splicing complex with nucleolin and hnRNPK to facilitate the production of a non‐protein‐coding inositol monophosphatase domain containing 1 (IMPAD1) splice variant, instead of the protein‐coding variant. The BC‐mediated alternative splicing (AS) of IMPAD1 resulted in the induction of the epithelial–mesenchymal transition and resistance to EGFR‐TKI in lung cancer. High BC expression correlated with clinical progress and poor survival among 402 patients with LUAD.DisscussionThrough alternative splicing, BC boosted the non‐coding IMPAD1‐203 transcript variant while suppressing the IMPAD1‐201 variant. In order to control the processing of pre‐mRNA, BC not only attracted RNA binding proteins (NCL, IGF2BP1) or splicing factors (hnRNPK), but also controlled the formation of the splicing‐regulator complex by creating RNA‐RNA‐duplexes.ConclusionOur results reveal an important role for BC in mediating resistance to EGFR‐targeted therapy in LUAD through IMPAD1 AS and in implication for the targeted therapy resistance.
Geranylgeranyl diphosphate synthase: Role in human health, disease and potential therapeutic target
Authors: Muehlebach Molly E. , Holstein Sarah A. 
Published: January 2023

AbstractGeranylgeranyl diphosphate synthase (GGDPS), an enzyme in the isoprenoid biosynthesis pathway, is responsible for the production of geranylgeranyl pyrophosphate (GGPP). GGPP serves as a substrate for the post‐translational modification (geranylgeranylation) of proteins, including those belonging to the Ras superfamily of small GTPases. These proteins play key roles in signalling pathways, cytoskeletal regulation and intracellular transport, and in the absence of the prenylation modification, cannot properly localise and function. Aberrant expression of GGDPS has been implicated in various human pathologies, including liver disease, type 2 diabetes, pulmonary disease and malignancy. Thus, this enzyme is of particular interest from a therapeutic perspective. Here, we review the physiological function of GGDPS as well as its role in pathophysiological processes. We discuss the current GGDPS inhibitors under development and the therapeutic implications of targeting this enzyme.
The detoxification of ochratoxin A in wine and grape juice by different enzymes and evaluation of their effects on the quality
Authors: Wang Saiqun , Cai Rui , Liu Xiaoshuang , Qi Lige , Wang Leran , Yue Tianli , Yuan Yahong , Wang Zhouli 
Published: January 2023

AbstractOchratoxin A (OTA) is a mycotoxin produced by Aspergillus and Penicillium genera. It is hepatotoxic, nephrotoxic and carcinogenic. The growing contamination of OTA in wine and grape juice is a great threat to human. In this study, different enzymes were screened for the detoxification of OTA in wine and grape juice. The results indicated that seven enzymes had OTA degradation capacity in the buffer, and the degradation rate of carboxypeptidase A (CPA), lipase, pancreatin and thermolysin were 100%, 100%, 75.72%, and 73.45%, respectively. In grape beverages, pancreatin and CPA showed the most promising result with OTA degradation of 53.24% and 61.56% at 37°C. The LC‐MS/MS analysis further indicated that OTA produced nontoxic OTα and phenylalanine under treatment with three enzymes (carboxypeptidase A, lipase, and pancreatin). Furthermore, pancreatin and CPA had a clarifying effect on wine and grape juice, but had a slight effect on the organic acids and flavor components of them. It suggested that pancreatin and CPA could be used for OTA degradation in grape beverages.
Reliability analysis of computed tomography equipment using the q‐Weibull distribution
Authors: Fan Litian , Hu Zhi , Ling Qingqing , Li Hanwei , Qi Hongliang , Chen Hongwen 
Published: January 2023

AbstractInspired by the successful application of the q‐Weibull distribution in other research fields, we took the lead to use it in the field of medical devices in this work. The parameter estimation of the q‐Weibull distribution was performed using the probability plot method. The CT failure data from Nanfang Hospital in Guangzhou, China, were used to study the reliability of CT equipment at two levels: the CT system and its seven main components. In terms of evaluation accuracy, the mean squared error, Akaike's information criterion, and the determination coefficient were used to compare the accuracy of fitting of different distribution models. The results show that the accuracy of fitting the q‐Weibull distribution is higher than that of the Weibull distribution in terms of determination coefficient and mean squared error. When considering the complexity of the model, the fit accuracy of the Weibull distribution is better. The results were analyzed using reliability and failure rate plots. The q‐Weibull distribution gives a good fit for the failure data of the CT system and components. Though the Weibull distribution fits better in a few cases, the q‐Weibull distribution can describe the entire “bathtub curve” with only a set of parameters. The findings of this study can be extended to other medical devices.
Safety and efficacy of rapid withdrawal of antiseizure medications during long‐term video‐electroencephalogram monitoring in children with drug‐resistant epilepsy: A retrospective study
Authors: Wang Shuang , Wang Wen , Yu Guojing , Wan Lin , Fan Yuying , Wang Hongjie , Liu Tong , Ji Taoyun , Liu Qingzhu , Cai Lixin , Liu Xiaoyan 
Published: January 2023

AbstractObjectivePerforming long‐term video‐electroencephalographic monitoring (LTVEM) to obtain the ictal electroencephalogram (EEG) is important for presurgical evaluation. This study aimed at investigating the safety and efficacy of our protocol developed at Peking University First Hospital (PUFH) for rapid withdrawal of antiseizure medications (ASMs) during LTVEM to induce seizures in children with drug‐resistant epilepsy (DRE) exhibiting nondaily seizures.MethodsChildren with DRE who followed the PUFH protocol for rapid withdrawal of ASMs during LTVEM between 2018 and 2021 were enrolled. The occurrence of seizures, number of ASMs withdrawn, seizure onset time after ASM tapering initiation, changes in interictal epileptiform discharge (IED), and adverse events were evaluated during LTVEM.ResultsAmong 80 children evaluated in this study, seizures were induced successfully in 72 (90%) children. Furthermore, no change in IED sites was observed in these 72 children following the initiation of ASM tapering while 2 children exhibited secondary bilateral tonic‐clonic seizures. The median time from ASM tapering initiation to the onset of the first seizure was found to be 3 days (2–4), while the median number of ASMs withdrawn was 2 (1–2). Finally, 66 children (91.7%) had habitual seizures while 6 children had nonhabitual seizure semiology.SignificanceThe PUFH protocol can be used for the rapid withdrawal of ASMs during LTVEM in children with DRE. Using this protocol, ictal EEG patterns can be obtained in a relatively short time for most patients with fewer adverse effects during LTVEM, which may provide meaningful electro‐clinical information for presurgical evaluation.
Quality and stability evaluation of Guizhou spicy chicken treated with gamma irradiation during the storage period
Authors: Li Guolin , Lin Ping , Li Yongfu , He Yangbo , Liu Zhihui 
Published: January 2023

AbstractIrradiation has become a mature processing approach for the quality control of many agricultural products. The effects of gamma irradiation at four different doses (2, 4, 6, and 8 kGy) on microbial quality, Hunter's parameter, lipid oxidation, hydrolyzed amino acids (HAAs), and flavor in spicy chicken were investigated. After treatment, all samples were stored at 4°C. The microbiological results showed that the total viable bacteria (TVB) and total coliform count (TCC) were significantly decreased dose dependently. Values of Δa*, Δb*, and ΔE* of the irradiated specimens were lower compared with the control samples, whereas the ΔL* of the irradiated specimens was higher compared with the controls. The peroxide value (POV) was increased by dose augmentation. Contents of HAAs were gradually decreased in both irradiated and control groups. The odor was affected by both doses of irradiation and storage time. Hence, we conclude that irradiation at a dose of 4.0 kGy barely affected physicochemical properties during storage and extended the shelf life of spicy chicken. This approach could be an alternative to control the quality of spicy chicken during storage.
Healthcare personnel self‐assessed competence and knowledge following implementation of a new guideline on maternal resuscitation in Norway. A repeated measure study
Authors: Hardeland Camilla , Svendsen Edel J. , Heitmann Grethe B. , Leonardsen Ann‐Chatrin L. 
Published: January 2023

AbstractIntroductionCardiac arrest in pregnancy is a rare, yet extremely challenging condition to manage for all healthcare personnel involved. Knowledge deficits and poor resuscitation skills can affect outcomes in cardiac arrest in pregnancy, but research exploring healthcare personnel competence and knowledge about maternal resuscitation is limited.AimsThe aim of this study was to explore (1) healthcare personnel self‐assessed competence and knowledge about cardiopulmonary resuscitation (CPR) in pregnancy as well as perimortem caesarean section, before and after implementation of a new guideline, (2) whether there were any interprofessional differences in knowledge about maternal resuscitation, and (3) potential differences between different implementation strategies.Research MethodologyThe study had a prospective repeated measure implementation design, utilizing a questionnaire before and after implementation of a new guideline on maternal resuscitation after cardiac arrest.SettingAll healthcare personnel potentially involved in CPR in six hospital wards, were invited to participate (n = 527). The guideline was implemented through either simulation, table‐top discussions and/or an electronical learning course.ResultsIn total, 251 (48%) participants responded to the pre‐questionnaire, and 182 (35%) to the postquestionnaire. The need for education and training/simulation concerning maternal resuscitation were significantly lowered after implementation of the guideline, yet still the majority of respondents reported a high to medium need for education and training/simulation. Participants' self‐assessed overall competence in maternal resuscitation increased significantly postimplementation. Regardless of professional background, knowledge about CPR and perimortem caesarean section increased significantly in most items in the questionnaire after implementation. Differences in level of knowledge based on implementation strategy was identified, but varied between items, and was therefore inconclusive.ConclusionThis study adds knowledge about healthcare personnel self‐assessed competence and knowledge about maternal resuscitation and perimortem caesarean section in pregnancy. Our findings indicate that there is still a need for more education and training in this rare incident.
Pregnancy and the risk of NICU admissions in Nandom Municipality of Ghana: A cross‐sectional retrospective study
Authors: Ziem Maroun Soribang , Saaka Fidelis Adam , Vicar Ezekiel Kofi , Kuugbee Eugene Dogkotenge , Karikari Akosua Bonsu , Ninimiya Sebastian Yidana , Ziem Juventus Benogle , Walana Williams 
Published: January 2023

AbstractBackgroundNeonatal intensive care units (NICU) are specialized units that provide medical attention to neonates, and thus have become a vital aspect in the provision of critical care to infants who are faced with special challenges following birth.AimTo determine antepartum and intrapartum factors that predispose to NICU admissions in the Nandom Municipal of the Upper West Region of Ghana.MethodThis was a cross‐sectional retrospective study, spanning from January 1, 2021 to December 31, 2021. Records covering 1777 women who were delivered or had their babies referred to the St. Theresa's Hospital in the Nandom Municipality were involved in the study. Descriptive statistics and multinomial logistic regression analysis were used to compare variables, and statistical significance was determined where the p‐value was less than 0.05.ResultsFrom the study, the rate of NICU admission was 10.4%. There was a significant association between mothers who attended less than four antenatal sessions (p = 0.004) and admission to NICU. Nulliparous mothers (p = 0.027) and mothers who presented with multiple pregnancy (p < 0.001) were more likely to have their babies sent to NICU. Both preterm delivery (p < 0.001) and post‐term delivery (p < 0.001) were prone to admission to NICU. Also, instrumental delivery (p < 0.001), cesarean section (p < 0.001), low birth weight (p < 0.001), and male infants (p = 0.003) had an increased risk of being admitted to NICU. Furthermore, severe (p < 0.001) and moderate (p < 0.001) birth asphyxia in the first minute following delivery were significantly associated with NICU admission whereas severely asphyxiated babies at 5 min (p < 0.001) were associated with NICU admission.ConclusionThe study revealed a relatively high NICU admission rate in the study area, and the predictors are multifaceted. Tailored intervention programs aimed at curbing these predictors will be required to reduce the rate of NICU admissions in the Nandom Municipality of Ghana.
Determinants of vaccine acceptance, knowledge, attitude, and prevention practices against COVID‐19 among governmental healthcare workers in Addis Ababa and Adama, Ethiopia: A cross‐sectional study
Authors: Girmay Aderajew M. , Weldegebriel Mesaye G. , Serte Melaku G. , Dinssa Daniel A. , Alemayehu Tsigereda A. , Kenea Moa A. , Weldetinsae Abel , Teklu Kirubel T. , Mengesha Sisay D. , Alemu Zinabu A. , Demisie Belaynesh , Wagari Bedasa , Evans Martin R. , Tessema Masresha , Tollera Getachew 
Published: January 2023

AbstractBackground and AimsCOVID‐19 vaccines are vital tools for infection prevention and control of the pandemic. However, coronavirus immunization requires acceptance among healthcare workforces and by the community. In Ethiopia, studies focused on determinants of vaccine acceptance, knowledge, attitude, and prevention practices (KAP) contrary to the novel coronavirus among healthcare staff are limited. Hence, closing this gap requires research.MethodsA cross‐sectional study was conducted on 844 governmental healthcare workers. A stratified, simple random sampling technique was used to select the respondents. Data were collected using a structured questionnaire. Binary and multivariable logistic regression statistical models were used to analyze the data.ResultsThis study indicated that only 57.9% of the participants had good COVID‐19 vaccine acceptance, meaning they took at least a dose of the vaccine themselves. We found that 65%, 60.9%, and 51.3% of the participants had good knowledge, prevention practices, and attitude against the pandemic. The novel coronavirus vaccine acceptance rate was 2.19 times more likely among females (adjusted odds ratio [AOR] = 2.19 with 95% confidence interval [CI]: 1.54–3.10) than among male participants. Further, respondents who did not report having any chronic diseases were 9.40 times higher to accept COVID‐19 vaccines (AOR = 9.40 with 95% CI: 4.77, 18.53) than those who reported having a chronic condition. However, healthcare workers who had a habit of chewing khat at least once per week were 4% less likely to take the vaccine (AOR = 0.04 with 95% CI: 0.01, 0.32) than those who had no habit of chewing khat.ConclusionMany core factors influencing COVID‐19 vaccine acceptance were identified. A significant number of participants had poor vaccine acceptance, KAP against COVID‐19. Therefore, the government should adopt urgent and effective public health measures, including public campaigns to enhance public trust in COVID‐19 vaccines. In addition, continuous, timely, and practical training should be provided to healthcare workers.
Association of calcium and vitamin D supplementation with cancer incidence and cause‐specific mortality in Black women: Extended follow‐up of the Women's Health Initiative calcium‐vitamin D trial
Authors: Kato Ikuko , Sun Jun , Hastert Theresa A. , Abrams Judy , Larson Joseph C. , Bao Wei , Shadyab Aladdin H. , Mouton Charles , Qi Lihong , Warsinger Martin Lisa , Manson JoAnn E. 
Published: January 2023

AbstractLow circulating vitamin D levels are more prevalent in Black than White individuals. We analyzed the Women's Health Initiative (WHI) calcium plus vitamin D (CaD) randomized clinical trial extended follow‐up data to evaluate associations between calcium plus vitamin D supplementation and incident cancer, cardiovascular disease (CVD), and cause‐specific mortality endpoints among Black women. Intent‐to‐treat analysis was performed. Among 3325 Black women in the CaD trial who were randomized into either daily calcium (1000 mg of calcium carbonate) plus vitamin D (400 IU D3) or placebos for an average of 7 years, there were 813 deaths, 588 incident cancers, and 837 CVD events during an average of 15.7 years of follow up (52 230 total person‐years). Using Cox's proportional hazards models, we calculated hazard ratios and their confidence intervals for outcomes ascertained during the trial period, posttrial follow‐up period and overall periods combined. We found that total mortality, cause‐specific mortality, and total cancer incidence were almost identical between CaD and placebo groups. These results suggest that calcium plus vitamin D supplementation does not reduce risks of cancer, CVD, or other major causes of death in Black women overall and, thus, other medical, behavioral or social interventions should be considered to narrow health disparities related to these outcomes. However, other finer endpoints, such as colorectal cancer, warrants further investigation.