bluuebunny/crossref_metadata_2025_split huggingface dataset:
Cas9-mediated knockout of Ndrg2 enhances the regenerative potential of dendritic cells for wound healing
Authors: Henn Dominic , Zhao Dehua , Sivaraj Dharshan , Trotsyuk Artem , Bonham Clark Andrew , Fischer Katharina S. , Kehl Tim , Fehlmann Tobias , Greco Autumn H. , Kussie Hudson C. , Moortgat Illouz Sylvia E. , Padmanabhan Jagannath , Barrera Janos A. , Kneser Ulrich , Lenhof Hans-Peter , Januszyk Michael , Levi Benjamin , Keller Andreas , Longaker Michael T. , Chen Kellen , Qi Lei S. , Gurtner Geoffrey C. 
Published: August 2023

AbstractChronic wounds impose a significant healthcare burden to a broad patient population. Cell-based therapies, while having shown benefits for the treatment of chronic wounds, have not yet achieved widespread adoption into clinical practice. We developed a CRISPR/Cas9 approach to precisely edit murine dendritic cells to enhance their therapeutic potential for healing chronic wounds. Using single-cell RNA sequencing of tolerogenic dendritic cells, we identified N-myc downregulated gene 2 (Ndrg2), which marks a specific population of dendritic cell progenitors, as a promising target for CRISPR knockout. Ndrg2-knockout alters the transcriptomic profile of dendritic cells and preserves an immature cell state with a strong pro-angiogenic and regenerative capacity. We then incorporated our CRISPR-based cell engineering within a therapeutic hydrogel for in vivo cell delivery and developed an effective translational approach for dendritic cell-based immunotherapy that accelerated healing of full-thickness wounds in both non-diabetic and diabetic mouse models. These findings could open the door to future clinical trials using safe gene editing in dendritic cells for treating various types of chronic wounds.
Seasonal variation in defense behavior in European and scutellata-hybrid honey bees (Apis mellifera) in Southern California
Authors: Zarate Daniela , Mukogawa Brandon , Kohn Joshua , Nieh James C. 
Published: August 2023

AbstractNest defense in the honey bee (Apis mellifera) is a complex collective behavior modulated by various interacting social, environmental, and genetic factors. Scutellata-hybrid (“Africanized”) honey bees are usually considered to be far more defensive than European honey bees which are therefore preferred for commercial and hobbyist beekeeping. In the most recent zone of scutellata hybridization, the southern USA, the degree to which this defensiveness differs among current strains, and the extent to which defensiveness varies across a season has not been measured. We quantified the levels of A. m. scutellata ancestry in colonies and conducted a seasonal assessment (May through November) of colony nest defensiveness in feral scutellata-hybrid and a popular lineage of European honey bee commonly used in managed environments (sold as A. mellifera ligustica) hives at two apiaries in Southern California. Standard measures of defensiveness were low in both scutellata-hybrid and European colonies during May. Defensiveness increased during the later months of the study in scutellata-hybrid colonies. Most measures of defensiveness did not increase in managed colonies. Defensiveness in the scutellata-hybrids appears lower than what has been previously documented in Brazil and Mexico, possibly due to their lower proportion of A. m. scutellata ancestry.
Wood sawdust waste-derived nano-cellulose as a versatile reinforcing agent for nano silica cement composites: a systematic study on its characterization and performance
Authors: Badawy Amr H. , El-Feky M. S. , El-Tair A. Maher , Kohail Mohamed 
Published: August 2023

AbstractThe development of sustainable construction materials is a pressing concern for researchers worldwide, as the cement industry is a major contributor to environmental degradation. The incorporation of nano-materials with cement composites has emerged as a promising solution to sustainable materials production. In this study, the effect of the addition of nano cellulose produced from wood sawdust waste on the performance of cement-based nano-silica composite was investigated. The nano-materials were incorporated at low concentrations and in gel form to eliminate the need for any advanced dispersion techniques. The results indicated that the addition of even low concentrations of nano cellulose significantly enhanced the compactness and mechanical properties of the cement matrix. The crack propagation was observed to be arrested with better adherence to the cement hydration product, which resulted from the presence of nano-silica. The nano cellulose fibers were found to bridge the calcium silicate hydrate products, arresting the propagation of cracks at their initial condition. The high pozzolanic reactivity of nano-silica ensured a minimal amount of calcium hydroxide, which is a significant contributor to the carbon footprint of cement production. Overall, the findings of this study suggest that the incorporation of nano cellulose from wood sawdust waste with cement-based nano-silica composite can lead to the development of sustainable and high-performance building materials with improved mechanical properties and reduced environmental impact.
Molecular modeling study on the water-electrode surface interaction in hydrovoltaic energy
Authors: Serdaroğlu Goncagül , Kariper İ. Afşin , Kariper S. Esra Bolsu 
Published: August 2023

AbstractThe global energy problem caused by the decrease in fossil fuel sources, which have negative effects on human health and the environment, has made it necessary to research alternative energy sources. Renewable energy sources are more advantageous than fossil fuels because they are unlimited in quantity, do not cause great harm to the environment, are safe, and create economic value by reducing foreign dependency because they are obtained from natural resources. With nanotechnology, which enables the development of different technologies to meet energy needs, low-cost and environmentally friendly systems with high energy conversion efficiency are developed. Renewable energy production studies have focused on the development of hydrovoltaic technologies, in which electrical energy is produced by making use of the evaporation of natural water, which is the most abundant in the world. By using nanomaterials such as graphene, carbon nanoparticles, carbon nanotubes, and conductive polymers, hydrovoltaic technology provides systems with high energy conversion performance and low cost, which can directly convert the thermal energy resulting from the evaporation of water into electrical energy. The effect of the presence of water on the generation of energy via the interactions between the ion(s) and the liquid–solid surface can be enlightened by the mechanism of the hydovoltaic effect. Here, we simply try to get some tricky information underlying the hydrovoltaic effect by using DFT/B3LYP/6-311G(d, p) computations. Namely, the physicochemical and electronic properties of the graphene surface with a water molecule were investigated, and how/how much these quantities (or parameters) changed in case of the water molecule contained an equal number of charges were analyzed. In these computations, an excess of both positive charge and negative charge, and also a neutral environment was considered by using the Na+, Cl−, and NaCl salt, respectively.
Controlling nutritional status score during hospitalization as a predictor of clinical outcome in patients with aneurysmal subarachnoid hemorrhage
Authors: Shimizu Shinya , Hanai Tatsunori , Egashira Yusuke , Sato Yukina , Sekiya Kumiko , Nishida Shohei , Ishihara Masashi , Ishihara Takuma , Asada Ryuta , Kobayashi Ryo , Suzuki Akio 
Published: August 2023

AbstractAneurysmal subarachnoid hemorrhage (aSAH) is a serious condition with high mortality and a high permanent disability rate. In this study, we examined the association of clinical outcome with the Controlling Nutritional Status (CONUT) score during hospitalization in aSAH patients. A single-center, retrospective observational study was conducted at Gifu University Hospital. Patients transported to the emergency room for aSAH and diagnosed with World Federation of Neurosurgical Societies (WFNS) grade III and IV aSAH between April 2004 and March 2021 were enrolled. A logistic regression model was constructed to evaluate the association of the CONUT score with a modified Rankin scale (mRS) ≥ 3 and delayed cerebral ischemia (DCI). 127 patients diagnosed with WFNS grade III and IV aSAH were analyzed. CONUT score was significantly associated with mRS ≥ 3 during hospitalization. The score obtained by subtracting the CONUT score at admission from the maximum CONUT score was significantly associated with mRS ≥ 3 at discharge. Moreover, the score obtained by subtracting the CONUT score at admission from the maximum CONUT score during the first 14 days was significantly associated with DCI within 14 days from admission. These findings indicate that CONUT score during hospitalization may be a useful daily marker for predicting poor outcomes in aSAH patients.
Observational study of surgical resection in small non-functional pancreatic neuroendocrine tumors: AS SEER-based study
Authors: Lin Jiajing , Huang Heguang 
Published: August 2023

AbstractThe potential benefits of surgical resection for small non-functional pancreatic neuroendocrine tumors (NF-PNETs) in terms of survival remain uncertain. This study aimed to evaluate the impact of surgical treatment on patients with NF-PNETs. Using SEER data, we identified 1102 patients from 2004 to 2015 with well and moderately differentiated pancreatic neuroendocrine tumors (PNETs). The associations between continuous variables and receipt of surgery were assessed using Wilcoxon rank-sum tests. Kaplan–Meier survival curves for OS were compared using the log-rank test. We compared outcomes in patients who received surgical resection with those in patients who did not, using a univariable Cox model with inverse probability weighting according to the propensity score and propensity-score matching. Among the cohort of 1102 patients, a majority of 965 individuals (87%) underwent surgical intervention. Upon conducting univariate analysis, we observed that surgical treatment significantly prolonged patients' survival [HR = 0.41, 95% CI [0.26–0.65] P < 0.001]. However, the old [HR = 3.27, 95% CI (2.24–4.76), P 0.001], male gender [HR = 1.82, 95% CI (1.23–2.68), P = 0.003], and moderately well-differentiated factors [HR = 1.71, 95% CI (1.04–2.80), P = 0.034] were found to potentially decrease patients' survival time. In the multivariate analysis, male gender [HR = 1.73, 95% CI (1.15–2.61), P = 0.009] and the old factor [HR = 3.52, 95% CI (2.33–5.31), P < 0.001] emerged as influential predictors with higher hazard ratios. Notably, surgical treatment remained a significant factor associated with improved overall survival [HR = 0.53, 95% CI (0.33–0.84), P = 0.007]. Propensity-score matching and inverse probability weighting were employed as analytical techniques. The univariate analysis results showed favorable outcomes in the weight group [HR = 0.48, 95% CI (0.29–0.78), P = 0.003] and matched group [HR = 0.44, 95% CI (0.22–0.85), P = 0.015], respectively. Survival analysis further confirmed that surgical treatment contributed to increased overall survival (log rank, P < 0.05) in both the matching and weight groups. Patients diagnosed with small, non-functioning pancreatic neuroendocrine tumors who undergo surgical intervention exhibit improved overall survival (OS) outcomes. Therefore, surgery is strongly recommended for this patient population.
Tibetan Plateau increases the snowfall in southern China
Authors: Wang Liping , Yang Haijun 
Published: August 2023

AbstractThe role of the Tibetan Plateau (TP) in Asian hydrological climate is crucial, yet there is a lack of quantitative estimates regarding its impact on snowfall in China. Some opinions suggest that the TP functions as a large barrier that obstructs cold outbreaks, protecting southern China from severe snowstorms. Through topography experiments with and without the TP, our study suggests that the TP's presence results in a 60% decrease in snowfall in northern China by significantly reducing moisture. In contrast, it promotes a 1500% increase in snowfall in southern China, particularly from November to March, by drawing cold air from the north and moisture from the south to southern China. The presence of TP significantly enhances winter relative humidity in southern China, causing discomfort for humans. This research refutes some trending views and improves our understanding of the TP's role in China's winter climate.
Transcriptome and WGCNA reveal hub genes in sugarcane tiller seedlings in response to drought stress
Authors: Tang Yuwei , Li Jiahui , Song Qiqi , Cheng Qin , Tan Qinliang , Zhou Quanguang , Nong Zemei , Lv Ping 
Published: August 2023

AbstractDrought stress can severely affect sugarcane growth and yield. The objective of this research was to identify candidate genes in sugarcane tillering seedlings in response to drought stress. We performed a comparative phenotypic, physiological and transcriptomic analysis of tiller seedlings of drought-stressed and well-watered “Guire 2” sugarcane, in a time-course experiment (5 days, 9 days and 15 days). Physiological examination reviewed that SOD, proline, soluble sugars, and soluble proteins accumulated in large amounts in tiller seedlings under different intensities of drought stress, while MDA levels remained at a stable level, indicating that the accumulation of osmoregulatory substances and the enhancement of antioxidant enzyme activities helped to limit further damage caused by drought stress. RNA-seq and weighted gene co-expression network analysis (WGCNA) were performed to identify genes and modules associated with sugarcane tillering seedlings in response to drought stress. Drought stress induced huge down-regulated in gene expression profiles, most of down-regulated genes were mainly associated with photosynthesis, sugar metabolism and fatty acid synthesis. We obtained four gene co-expression modules significantly associated with the physiological changes under drought stress (three modules positively correlated, one module negatively correlated), and found that LSG1-2, ERF1-2, SHKA, TIL, HSP18.1, HSP24.1, HSP16.1 and HSFA6A may play essential regulatory roles as hub genes in increasing SOD, Pro, soluble sugar or soluble protein contents. In addition, one module was found mostly involved in tiller stem diameter, among which members of the BHLH148 were important nodes. These results provide new insights into the mechanisms by which sugarcane tillering seedlings respond to drought stress.
Aging and temporal integration in the visual perception of object shape
Authors: Norman J. Farley , Lewis Jessica L. , Bryant Emily N. , Conn Juma D. 
Published: August 2023

AbstractIt has been known for more than 160 years that highly occluded objects that would normally be visually unrecognizable can be successfully identified when they move. This anorthoscopic perception relies on the visual system’s ability to integrate information over time to complete the perception of an entire object’s shape. In this experiment, 16 younger and older adults (mean ages were 20.5 and 74.6 years, respectively) were familiarized with the (unoccluded) shapes of five naturally-shaped objects (bell peppers, Capsicum annuum) until they could be easily identified (i.e., with accuracies of at least 90 percent correct). All observers then viewed the stimulus objects anorthoscopically as they moved behind narrow slits; only small object fragments could be seen at any given time, because the objects were almost totally occluded from view. Even though the object identification performance for all observers was equivalent when whole object shapes were visible, a large age-related deficit in object identification emerged during anorthoscopic viewing such that the younger adults’ identification performance was 45.4 percent higher than that of the older adults. This first ever study of aging and anorthoscopic perception demonstrates that there is an age-related deficit in performing the temporal integration needed for successful object recognition.
Parameters of flow asymmetrically constrained by through spurs
Authors: Bakiev Masharif , Matkarimov Otanazar , Khalimbetov Azat , Kuybakov Seytkhan 
Published: August 2023

Erosion of the banks of rivers brings enormous damage to the national economy of many countries. To combat them, various regulatory structures are being built: longitudinal dams, transverse traverse dams, watertight and permeable spurs, and combined transverse dams. Many issues of their design are sufficiently developed, including in our works, and are successfully applied.
This research aims to develop a method for hydraulic flow asymmetrically constrained by through pile-type spurs. The conditions of the planned task of the ratio of the flow width to the depth of more than 6 were also observed. The degree of restriction of the flow by spurs varied from zero to one, and the installation angle of the spurs from 600 to 900. The study of the velocity field showed their similarity with the main provisions of the theory of turbulent jets. The velocity distributions in both zones of turbulent mixing are universal and obey the theoretical Schlichting- Abramovich dependence. The expansion coefficient of the jet is 0.20. Theoretical studies have obtained dependences for determining the velocity in a weakly disturbed core, occurrent flows, and the spreading regions' lengths, which determine the installation locations of subsequent spurs.
Dependence of stress-strain state of hydraulic structures on infiltration moistening of foundation soils
Authors: Xujakulov R. , Samandarova G. 
Published: August 2023

As is known, loess subsidence soils are widespread on the globe; their area in Europe and Asia is more than 13 million sq. km. Many of the loess rocks are also located in Central Asia, including the Republic of Uzbekistan. The practice of irrigation development of loess lands shows that the disruptions that have occurred so far in the operation of hydraulic structures (HTS) are due to insufficient knowledge and neglect in designing the features of joint work and interaction structures with their subsidence foundations. The design of hydraulic structures also does not fully consider these features. To ensure the long-term, reliable, and economical operation of hydraulic structures on subsiding soils, it is necessary to improve their design and construction methods, considering the peculiarities of interaction with loess foundations. This article deals with the features of infiltration soil moistening from channels and structures on them.
The article deals with the features of the infiltration moistening of soils from canals and structures on them. Based on the analysis of previous studies, the specificity of infiltration in loess soils is considered a serious factor affecting the stress-deformed state of hydraulic structures and their foundations.
Management of consumers needs for volume of transportation, taking into account the probable nature
Authors: Zhuraev Mukhiddin , Togaev Jamshid , Yusufkhonov Zokirkhon 
Published: August 2023

The article investigates the issue of continuous provision of consumers' needs for transportation, which is formed by chance. At the same time, the indicators of elementary random processes aimed at satisfying the needs of consumers in transportation were investigated, and corresponding mathematical models were formed for them; methodological properties and aspects of this approach were also substantiated. Based on the cost of transporting raw cotton materials from the Nuriston cotton station to the Nishan cotton ginnery, the reliability of the results of operational planning of transportation and transportation management is considered.
Based on the mathematical models presented in the article, it becomes possible to continuously meet the production needs of an enterprise at any random cost of consumed and transported goods.
Structural-phase transformations during nitro oxidation of steels and surface properties
Authors: Eshkabilov Kholikul 
Published: August 2023

Structural and phase changes in the surface diffusion layer during gas nitriding of steel were studied, with the formation of a crystallographic structure of the nitride layer for subsequent oxidation in water vapor. The conditions for forming carbides and nitrocarbides with simultaneous diffusion of nitrogen and decarburization of the steel matrix are determined depending on the nitrogen potential of the saturating medium.
The structural-phase states of the surface diffusion layer with a stepwise change in the nitrogen potential during nitriding at the first stage and followed by oxidation at the second stage, the formation of a surface oxide layer, as well as the conditions for participation in mutual diffusion processes of carbon in the nitride layer, decarburized from the steel matrix with the formation compositions of modified nitride phases.
The nitride-oxide layer’s corrosion properties and gradient properties in various structural-phase states of nitride phase compositions have been studied.
Diagnostics system for plant leaf diseases using photo images
Authors: Muhamediyeva Dilnoz , Tukhtamurodov Nozir 
Published: August 2023

The subject under consideration is the issue of detecting agricultural crop diseases. The preliminary data for determining the phytosanitary status of cultivated plants are the images of their leaves. To tackle this issue, a model of diagnostic algorithms has been proposed, which involves creating a set of preferred characteristics and making diagnostic decisions based on comparing these features. The process of establishing the model of diagnostic algorithms has been outlined. The effectiveness of the proposed model has been demonstrated in diagnosing wheat diseases based on leaf images.
A novel technique for detecting plant diseases has been introduced, relying on digital RGB photographs of leaves in the visible spectrum. The novelty of this method lies in diagnosing visible symptoms of plant leaf diseases from photographic images. There is a significant number of plant diseases that can reduce productivity, resulting in economic and environmental damage. Consequently, the accurate and timely diagnosis of plant diseases is paramount. One widely used approach for detecting plant pathologies involves using convolutional neural networks to diagnose plant leaf diseases from photographic images.
Formation of diffusion nitride-oxide coatings
Authors: Boynazarov Urol 
Published: August 2023

It is known that nitro-oxide coatings were developed based on nitriding, which are obtained using a two-stage technology consisting of nitriding at the first stage of the process and vaporization at the second stage. Such processing provides high physical and mathematical characteristics of hardened parts.
This technological process was improved by us due to preliminary oxidation, which made it possible to accelerate the production of nitride-oxide with the necessary physical and mechanical properties.
This work makes a theoretical calculation of the oxidation processes and subsequent nitriding of steels and a comparison with experimental data on the example of 38X2MYA. The article presents an analysis of the formation of oxynitride diffusion protective layers obtained by a three-stage nitriding method at a temperature of 580° C, which consists of preliminary oxidation, nitriding, and subsequent steam oxidation. The article also describes the effect of the oxide film on the nitriding process during preliminary oxidation and nitriding under short-term conditions at a temperature of 580 S.