bluuebunny/crossref_metadata_2025_split huggingface dataset:
Irisin-loaded electrospun core-shell nanofibers as calvarial periosteum accelerate vascularized bone regeneration by activating the mitochondrial SIRT3 pathway
Authors: Hua Xi , Hou Mingzhuang , Deng Lei , Lv Nanning , Xu Yong , Zhu Xuesong , Yang Huilin , Shi Qin , Liu Hao , He Fan 
Published: July 2023

Abstract
The scarcity of native periosteum poses a significant clinical barrier in the repair of critical-sized bone defects. The challenge of enhancing regenerative potential in bone healing is further compounded by oxidative stress at the fracture site. However, the introduction of artificial periosteum has demonstrated its ability to promote bone regeneration through the provision of appropriate mechanical support and controlled release of pro-osteogenic factors. In this study, a polylactic acid (PLLA)/hyaluronic acid (HA)-based nanofibrous membrane was fabricated using the coaxial electrospinning technique. The incorporation of irisin into the core-shell structure of PLLA/HA nanofibers (PLLA/HA@Irisin) achieved its sustained release. In vitro experiments demonstrated that the PLLA/HA@Irisin membranes exhibited favorable biocompatibility. The osteogenic differentiation of bone marrow mesenchymal stem cells (BMMSCs) was improved by PLLA/HA@Irisin, as evidenced by a significant increase in alkaline phosphatase (ALP) activity and matrix mineralization. Mechanistically, PLLA/HA@Irisin significantly enhanced the mitochondrial function of BMMSCs via the activation of the sirtuin 3 antioxidant pathway. To assess the therapeutic effectiveness, PLLA/HA@Irisin membranes were implanted in situ into critical-sized calvarial defects in rats. The results at four and eight weeks post-surgery indicated that the implantation of PLLA/HA@Irisin exhibited superior efficacy in promoting vascularized bone formation, as demonstrated by the enhancement of bone matrix synthesis and the development of new blood vessels. The results of our study indicate that the electrospun PLLA/HA@Irisin nanofibers possess characteristics of a biomimetic periosteum, showing potential for effectively treating critical-sized bone defects by improving the mitochondrial function and maintaining redox homeostasis of BMMSCs.
Robust Adaptive Beamforming for Coprime Array Based on Subspace Orthogonality
Authors: Huang Xiangdong , Hu Nian , Yang Xiaoqing , Gan Lin 
Published: July 2023

Abstract

This paper presents a robust adaptive beamforming (RAB) algorithm for coprime array based on subspace orthogonality.Initially, we eliminate residual noise to improve the accuracy of the noise power estimation. The Desired Signal Steering Vector (DSSV) is derived from the noise-free desired Sample Covariance Matrix (SCM). To obtain a virtual Uniform Linear Array (ULA) much longer than the actual linear array, we derive the continuous elements in the difference coarray and construct the Toeplitz matrix to create the virtual SCM. The interference Steering Vectors (SVs) are optimized by exploiting the orthogonality between the complementary subspace and the actual interference SVs. Subsequently, we accurately reconstruct the Interference-plus-Noise Covariance Matrix (INCM) in the physical domain, utilizing the maximum correlation principle, and derive the optimal weight vector for beamforming.Through simulation results, we demonstrate the robustness of the proposed algorithm in dealing with look direction errors, incoherent local scattering errors, array element gain and phase perturbation errors.
A Bioinformatics-Based Analysis of an Cuproptosis and Ferroptosis-Related Gene Signature Predicts the Prognosis of Patients with lung adenocarcinoma
Authors: Liu Xizhi , Gu Shanzhi , Zhao Xinhan , Zhang Yujiao 
Published: July 2023

Abstract
Background
Cuproptosis and ferroptosis acts important defense for the organism by preventing tumor cells migration and preventing their growth. In this study, cuproptosis and ferroptosis-related genes were used to construct a prognostic model for lung adenocarcinoma (LUAD) patients.
Methods
TCGA database was used to acquire RNA sequencing data and clinical information for LUAD samples. The Cox and LASSO regression analysis were performed to construct the prognostic genes signature. In addition, GSEA, GO, KEGG were performed to investigate the potential molecular mechanism. Moreover, we analyzed the relationship between our identified signature and immune cell infiltration, tumor microenvironment, immunotherapy response, drug sensitivity analysis.
Results
Three prognosis related genes were selected (SRXN1, GLS2, SLC2A1). Finally, in vitro experiments we performed qRT-PCR, western blot, scratch test, colony-formation, lipid ROS analysis to validate the expression and function of SRXN1 gene.
Conclusion
Combined with clinicopathological characteristics, the risk model was validated as a new independent prognostic factor for LUAD.
Biocontrol agents establishment and their impact on rhizosphere microbiome and induced grapevine defenses is highly soil-dependent
Authors: Leal Catarina , Eichmeier Ales , Štůsková Kateřina , Armengol Josep , Bujanda Rebeca , Fontaine Florence , Trotel-Aziz Patricia , Gramaje David 
Published: July 2023

Abstract
Background
Without effective chemical treatments, there is an increased interest on biological control of plant diseases. Trichoderma and Bacillus species have shown biocontrol potential against several grapevine diseases. However, few studies have investigated the impact of introducing beneficial microorganisms in rhizosphere, on the indigenous soil existent microbiome. Plant-associated microbiomes are complex, and there is a limited knowledge of the mechanisms that maintain and stimulate specific plant-associated microbial communities. In this study, we tested the hypothesis that the introduction of the biological control agents (BCAs) Bacillus subtilis PTA-271 (Bs PTA-271) and Trichoderma atroviride SC1 (Ta SC1) produce distinctive modifications in the composition and co-occurrence network structure of the grapevine rhizosphere microbial community, as well as grapevine induced defenses.
Results
Our results demonstrated that, the fungal microbiome is more affected by factors such as soil type, BCA treatment, and sampling time than bacterial microbiome. Specifically, Ta SC1 application produced a negative impact on fungal diversity, while applications of BCAs did not affect bacterial diversity. Interestingly, the survival and establishment of both BCAs showed opposite trends depending on the soil type, indicating that the physicochemical properties of soils have a role on BCA establishment. Fungal co-occurrence networks were less complex than bacterial networks, but highly impacted by Ta SC1 application. Soils treated with Ta SC1, presented more complex and stable co-occurrence networks, with a higher number of positive correlations. Induced grapevine defenses also differed according to the soil, being more affected by BCA inoculation on sandy soil.
Conclusions
The findings of this research emphasize the complex relationships among microorganisms in the rhizosphere, and point to the impact of biocontrol treatments on fungal and bacterial rhizosphere microbiomes. It highlights the significance of taking into account various factors like soil type, sampling time, and BCA treatment, and their influence on the structure and dynamics of microbial communities. In the future, it is crucial to conduct additional studies to explore these microbial interactions in greater detail, with the goal of developing more precise and efficient biocontrol strategies for agriculture.
The Power of Youth: The Impact of the "Fridays for Future" Climate Movement on Voters, Politicians, and the Media
Authors: Waldinger Maria , Rainer Helmut , Ludwig Markus , Flückiger Matthias , Wichert Sebastian , Fabel Marc 
Published: July 2023

Abstract

We study the impact of the Fridays for Future climate protest movement in Germany on citizen political behavior. Throughout 2019, large crowds of young protesters, the majority of whom were under voting age, skipped school to demand immediate climate change mitigation measures. We exploit cell phone-based mobility data and hand-collected information on nearly 4,000 climate protests to construct a spatially and temporally highly disaggregated measure of protest participation. Then, using various empirical strategies to address the issue of nonrandom protest participation, we show that the local strength of the climate movement led to more Green Party votes in state-level and national-level elections during 2019 and after. We provide evidence suggesting that three mechanisms were simultaneously at play: reverse intergenerational transmission of pro-environmental attitudes from children to parents, stronger climate-related social media presence by Green Party politicians, and increased coverage of environmental issues in local media. Together, our results suggest that environmental protests by those too young to vote may provide some of the impetus that is needed to push society toward overcoming the climate trap.
The mitochondrial-generated lncRNAs mediate a mitochondria-to-nucleus retrograde regulation after their nuclear translocation
Authors: Li Jia , Bai Ruolin , Zhao Yongyun , Li Ling , Song Xu 
Published: July 2023

Abstract
Since an intense communication between nucleus and mitochondria is vital for maintaining cellular homeostasis, it is important to characterize biological molecules involved in the inter-compartmental crosstalk. While a number of nuclear-encoded lncRNAs (nulncRNAs) have been implicated in anterograde regulation from the nucleus to mitochondria, the participation of mitochondrial-derived long non-coding RNAs (mtlncRNAs) in retrograde regulation from mitochondria to nucleus remains poorly understood. In this study, we identified three mtlncRNAs, namely MDL1AS, lncND5, and lncCyt b, as retrograde messengers following their translocation to the nucleus. Facilitated by the RNA-binding proteins HuR and PNPT1, these three mtlncRNAs shuttle from the mitochondria to the nucleus, subsequently regulating a network of nuclear genes. Furthermore, we demonstrated the cooperative interaction between the nuclear-localized lncCyt b and the splicing factor hnRNPA2B1, which influences various aspects of cell metabolism, including glycolysis. This effect is potentially mediated through their synergistic impact on the pre-mRNA splicing of related nuclear genes. Moreover, our study expands our understanding of mitochondrial biology and provides novel insights it provides novel insights into the role of mtlncRNAs in mediating the communication between mitochondria-nucleus.
Probing the effect of the COVID-19 pandemic on the day and night land surface temperatures using MODIS data over Andhra Pradesh and Telangana states of India
Authors: Mogaraju Jagadish Kumar 
Published: July 2023

Abstract
This work mainly aimed at understanding the actual effects of the COVID-19 pandemic on Land Surface Temperature (LST) in terms of daytime LST (DLST) and nighttime LST (NLST). MODIS (MOD11A2) derived images from 2017 through 2022, excluding December, were used in this study. GIS maps were prepared and interpreted to know LST fluctuations along with the directions to yield better insights. Time series plots were presented to know the LST fluctuations across the time frame, i.e., 2017 to 2022. The pre-COVID and post-COVID (including COVID) period is considered in this study without using data earlier than 2017 to avoid the noise that might be generated. The correlation analysis was done, and it is observed that daytime LST during the COVID period (2020 and 2021) has witnessed DLST close to the years 2017 (0.93 and 0.92) and 2019 (0.95), and night time LST close to the year 2017 (0.93). This work supports the notion that the COVID pandemic has lowered LST overall and highlights that the actual effect is minimal in the study area.
The assessment of the effectiveness of the unimodal and bimodal models to evaluate the water flow through nature-based solutions substrates
Authors: Turco Michele , Palermo Stefania Anna , Polizzi Ada , Presta Ludovica , Pirouz Behrouz , Piro Patrizia 
Published: July 2023

Abstract

Nature-based solutions are popular techniques for managing stormwater. Most of them allow porous media as their main layer. The description of the Soil Water Retention Curve (SWRC) as the Unsaturated Hydraulic Conductivity Curve (UHCC) is often required to run the hydrological simulations with the physically based models. Using the unimodal and bimodal models to assess the SWRC and UHCC of soils is a widespread technique but their evaluation is often present in literature only in terms of curve fitting. Based on these assumptions, this work presents the performance assessment of the van Genuchten unimodal and bimodal models by functional evaluation of them based on the runoff from several substrates. Four substrates were investigated to define the structure, the SWRC, and the UHCC. Results showed that all substrates had a bimodal behaviour with lowest values of RMSE (RMSE_Θ = 0.0023 to 0.0037, RMSE_K = 0.0636 to 0.1284). Finally, a numerical simulation using the HYDRUS-1D model was performed for a three-month data set to check the effectiveness of the unimodal model instead of the bimodal one. The findings have shown that the unimodal model must be preferred instead of the bimodal because it has fewer parameters and assured low discrepancies in runoff volume (ε=0.00% to 6.25%).
Fenología de diez cultivares de Cannabis sativa L. bajo las condiciones ambientales de Palomino Guajira
Authors: Candelario-Guerrero David A. , Calabria Parodi Luis Eduardo , Pardey-Rodríguez Catherine , Vargas- Sanchez Jhon Jairo 
Published: July 2023

Los estudios sobre fenología de Cannabis sativa L. son una herramienta al momento de realizar prácticas de manejo del cultivo como la aplicación de fertilizantes, el manejo de plagas y enfermedades, y para valorar la adaptación del cultivo a las condiciones climáticas. La fenología estudia la ocurrencia de las fases en el ciclo de vida de las plantas, para su determinación es necesario el uso de la escala BBCH que codifica las etapas fenológicas días después del trasplante. El objetivo del trabajo fue evaluar el comportamiento de 10 genotipos de Cannabis sativa L. bajo condiciones de invernadero en etapas vegetativa, floración y cosecha para identificar los genotipos con la mejor adaptación a las condiciones climáticas en el corregimiento de Palomino. Las variables a evaluar fueron: altura de la planta, número de brotes, número de inflorescencias, índice de cosecha y rendimiento expresado en flor seca. Los datos de altura, brotes e inflorescencia se registraron tres veces por semana durante 10 semanas y en la semana 10 se cuantifico la altura final. El índice de cosecha y rendimiento se estimó al momento de la cosecha. El diseño estadístico fue completamente al azar con 10 tratamientos y 6 repeticiones por tratamiento. Los resultados mostraron que, de 58 etapas fenológicas definidas para Cannabis, en un ciclo asexual solo se evalúan 19 etapas. Además, con los datos recolectados de ganancia de altura, brotes e inflorescencias, se puede predecir quienes serán los genotipos más rendidores. El rendimiento de flor seca está influenciado por la longitud de la planta, número de brotes y número de inflorescencias; igualmente, el índice de cosecha mostró ser un indicador al momento de determinar el rendimiento de cada genotipo. El genotipo mejor adaptado a la zona de estudio fue PS111 por la altura, la cantidad de brotes vegetativos, inflorescencias y el rendimiento.
Pharmaceutical Design of a Formulation of Enrofloxacin-Alginate and its Strategic
Dosage to Achieve Mutant Prevention PK/PD Ratios in Broiler Chickens
Authors: Gutiérrez Lilia , Monroy-Barreto Minerva , García-Guzmán Perla , Sumano Héctor 
Published: July 2023

Introduction:
The comparative pharmacokinetics (PK) and PK/pharmacodynamics (PD) ratios of a
new pharmaceutical design of enrofloxacin-alginate in dried beads (EADBs) and the reference enrofloxacin
10% solution was determined in broiler chickens. Also, the same parameters were determined after administering
enrofloxacin with a double dosing scheme (through drinking water and as an in-feed medication of
EADBs). 500 Arbor-Acres broiler chickens were randomly divided into five groups (n=100), adjusting in all
cases, a dose of 10 mg/kg based on water and feed intake as follows: group EADBsad-lib receiving enrofloxacin
through EADBs added to their feed as dressing; group EADBsbolus forcing the beads into the proventriculus using
a semi-rigid gavage; group Enroad-lib dosed through their drinking water; group Enrobolus also administered
into the proventriculus by gavage; group Enrow&f administering 5 mg/kg as EADBs in their feed, plus 5 mg/kg
of enrofloxacin through their drinking water.

Methods:
The PK parameters and the key PK/PD ratios were determined (Cmax/MIC and AUC0-24/MIC). Only
group Enrow&f could achieve the PK/PD ratios regarded as mutant-prevention.

Results:
This trial is the first one in which an in-feed medication of enrofloxacin, combined with water dosing,
can result in PK/PD parameters superior to those obtained after administering the drug through drinking water
at a dose of 10 mg/kg.

Conclusion:
Contrary to expectations, groups Enroad-lib and Enrobolus failed to achieve the desired PK/PD ratios
when the breakpoint was established at 0.5 μg/mL but did so when MIC was set at 0.1 μg/mL. In contrast,
EADBsbolus and Enrow&f achieved an adequate AUC0-24/MIC ratio for both MIC levels.
Progression in the Relationship between Exosome Production and
Atherosclerosis
Authors: Yang Yi , Luo Jinxi , Kang Yunan , Wu Wenqian , Lu Yajie , Fu Jie , Zhang Xiaoyun , Cheng Min , Cui Xiaodong 
Published: July 2023

Abstract:
Atherosclerosis (AS) is the leading cause of cardiovascular disease, causing a major
burden on patients as well as families and society. Exosomes generally refer to various lipid bilayer
microvesicles originating from different cells that deliver various bioactive molecules to the
recipient cells, exerting biological effects in cellular communication and thereby changing the
internal environment of the body. The mechanisms of correlation between exosomes and the disease
process of atherosclerosis have been recently clarified. Exosomes are rich in nucleic acid
molecules and proteins. For example, the exosome miRNAs reportedly play important roles in the
progression of atherosclerotic diseases. In this review, we focus on the composition of exosomes,
the mechanism of their biogenesis and release, and the commonly used methods for exosome extraction.
By summarizing the latest research progress on exosomes and atherosclerosis, we can
explore the advances in the roles of exosomes in atherosclerosis to provide new ideas and targets
for atherosclerosis prevention, diagnosis, and treatment.
LncRNA FTH1P3: A New Biomarker for Cancer-Related Therapeutic Development
Authors: Darvish Maryam 
Published: July 2023

Abstract:
Cancer is a persistent and urgent health problem that affects the entire world. Not long ago, regulatory biomolecules referred to as long noncoding RNAs (lncRNAs) might have value for their innate abundance and stability. These single-stranded RNAs potentially interfere with several physiological and biochemical cellular processes involved in many human pathological situations, particularly cancer diseases. Ferritin heavy chain1 pseudogene 3 (FTH1P3), a lncRNA that is ubiquitously transcribed and belongs to the ferritin heavy chain (FHC) family, represents a novel class of lncRNAs primarily found in oral squamous cell carcinoma. Further research has shown that FTH1P3 is involved in other malignancies such as uveal melanoma, glioma, esophageal squamous cell carcinoma, non-small cell lung cancer, breast cancer, laryngeal squamous cell carcinoma, and cervical cancer. Accordingly, FTH1P3 significantly enhances cancer symptoms, including cell proliferation, invasion, metastasis, chemoresistance, and inhibition of apoptosis through many specific mechanisms. Notably, the clinical data significantly demonstrated the association of FTH1P3 overexpression with poor prognosis and poor overall survival within the examined samples. Here, we summarize all the research published to date (13 articles) on FTH1P3, focusing on the biological function underlying the regulatory mechanism and its possible clinical relevance.
Ultrasound-mediated PLGA-PEI Nanobubbles Carrying STAT6 SiRNA
Enhances NSCLC Treatment via Repolarizing Tumor-associated
Macrophages from M2 to M1 Phenotypes
Authors: Shu Hong , Lv Wenhao , Ren Zhi-jian , LI Hui , Dong Tiantian , Zhang Yao , Nie Fang 
Published: July 2023

Background:
Tumor-associated macrophages (TAMs) are crucial for non-small cell lung
cancer (NSCLC) development.

Objective:
In this study, polylactic acid-co-glycolic acid (PLGA)-polyethylenimine (PEI) nanobubbles
(NBs) carrying STAT6 siRNA were prepared and combined with ultrasound-mediated nanobubbles
destruction (UMND) to silence the STAT6 gene, ultimately repolarizing TAMs from the M2 to the M1
phenotype, treating NSCLC in vitro.

Methods:
PLGA-PEI NBs-siRNA were prepared and characterised, and their respective ultrasound
imaging, biological stabilities and cytotoxicities were detected. Transfection efficiency was evaluated
by fluorescence microscopy and flow cytometry. Repolarization of THP-1-derived M2-like macrophages
was determined by qPCR and flow cytometry. NSCLC cells (A549) were co-cultured with transfected
M2-like macrophages or their associated conditioned medium (CM). Western blotting was used to
detect STAT6 gene silencing in M2-like macrophages and markers of epithelial and mesenchymal in
A549 cells. The proliferation of A549 cells was detected using CCK-8 and cell colony formation assays.
Transwell assays were used to detect the migration and invasion of A549 cells.

Results:
PLGA-PEI NBs-siRNA had an average size of 223.13 ± 0.92 nm and a zeta potential of about
-5.59 ± 0.97 mV. PLGA-PEI NBs showed excellent ultrasonic imaging capability in addition to biological
stability to protect siRNA from degradation. UMND enhanced PLGA-PEI NBs-STAT6 siRNA
transfection in M2-like macrophages, which made M2-like macrophages repolarize to M1-like macrophages
and prevented proliferation, migration, invasion and epithelial-mesenchymal transition (EMT)
in A549 cells.

Conclusion:
UMND enhanced PLGA-PEI NBs-STAT6 siRNA to repolarize TAMs from the M2 to the
M1 phenotype, thus treating NSCLC. These findings provide a promising therapeutic approach for enhancing
NSCLC immunotherapy.
Neutrophil-Lymphocyte Ratio and Red Blood Cell Distribution Width in
Patients with Atrial Fibrillation and Rheumatic Valve Disease
Authors: da Silva Rose Mary Ferreira Lisboa , Borges Lucas Espindula 
Published: July 2023

Abstract:
The lifetime risk of developing atrial fibrillation (AF) is 1 in 3 adults, resulting in a prevalence
of 2-4%. Rheumatic heart disease (RHD) is a frequent aetiology of valvular heart disease in lowand
middle-income countries. Between 21% and 80% of patients with mitral valve disease, especially
with stenosis, may have AF. Both these conditions, AF and RHD, present a state of persistent inflammation.
In turn, inflammation is a frequent cause of anisocytosis, which can be evidenced through the parameter
RDW (red bold cell distribution width). Factors associated with increased RDW are also known
as risk factors associated with a higher incidence of AF. RDW may have an independent role in the
pathogenesis of AF and the increased propensity of both thromboembolic and bleeding events. Another
marker involved in the incidence of AF is the neutrophil-lymphocyte ratio. This is also a marker of oxidative
stress and inflammation and is associated with a higher rate of AF recurrence. This review will
evaluate these biomarkers and their association with cardiovascular events in patients with AF and
RHD. The hypotheses and current debates about the relationship of biomarkers with the severity of
chronic valve dysfunction, with acute rheumatic carditis in the paediatric population, and with the presence
of thrombus in the left atrium will be discussed.