bluuebunny/crossref_metadata_2025_split huggingface dataset:
Genetic association analysis of rs662799 ( − 1131A > G) polymorphism of APOA5 gene with morphometric and physio-metric traits using multiplex PCR
Authors: Fatma Rafat , Chauhan Waseem , Riyaz Seema , Rasheed Khalid , Afzal Mohammad 
Published: February 2023

Abstract
Background
The apolipoprotein A5 (APOA5) gene, significantly expressed in liver, has been involved in regulation of triglyceride metabolism, plasma lipid levels, serum adipokine levels and cardiovascular traits. A single-nucleotide polymorphism rs662799 ( − 1131A > G), 2 Kb upstream in the promoter region of this gene, causes decrease in the concentration of the product coded by this gene; hence, it may be responsible for impairments in normal function of the gene, ultimately leading to disease condition. Keeping in view the importance of APOA5 gene, the aim of the present study was to examine the association of genetic variant rs662799 of APOA5 gene with two quantitative traits simultaneously, viz. body mass index and blood pressure.

Results
The study involved a population of 246 subjects from North Indian region. Measurements of morphometric and physio-metric parameters were recorded using standard measures. Genotyping of APOA5 gene polymorphism (rs662799) using Tetra-primer amplification refractory mutation system PCR was performed. Statistical analyses were carried out using MS-Excel and SigmaPlot, and significance level was setup as p < 0.05. The allelic distribution of rs662799 polymorphism in this population was 77% for major allele (A) and 23% for minor allele (G). Significant association of rs662799 with increased body mass index and blood pressure was observed, with the presence of allele G. Under recessive genetic model, rs662799 polymorphism conferred a 17.71-fold risk of elevated body mass index (OR = 17.71, p < 0.001, CI (95%) = 4.05–77.46), and for increase in blood pressure, 3.79- and 3.83-fold risk of systolic blood pressure and diastolic blood pressure (OR = 3.792, p = 0.023, CI (95%) = 1.25–11.509 and OR = 3.83, p = 0.012, CI (95%) = 1.375–10.68, respectively) was observed. Under dominant genetic model, it showed a 3.060-fold risk of increase in body mass index (OR = 3.060, p < 0.001, CI (95%) = 1.78–5.25).

Conclusions
G allele of rs662799 of APOA5 gene showed significant susceptible association with BMI and BP. This study may be helpful for clinicians and researchers to investigate the diagnostic and prognostic value of the gene in question.
A systematic review on the biological evaluation of Calotropis procera (Aiton) Dryand
Authors: Dogara Abdulrahman Mahmoud 
Published: February 2023

Abstract
Background
Humans have used plants as a safe and effective medicine for a wide range of ailments ever since the earliest days of civilization. Calotropis procera potential as a treatment for a variety of ailments has been known for quite some time. This xerophytic, upright shrub grows to a height of about 6 m and can be found in the tropics of Africa and Asia. Its parts have been used to cure a variety of ailments, including rheumatism, fever, dysentery, diabetes, malaria, asthma, and many more. Here, we provide a synopsis of the available biological data and discuss the possible ways in which Calotropis procera could be used as a novel platform for the treatment of a wide range of diseases.

Main body
High antioxidant, antiinflammatory, antianalgesic, antimicrobial, antimalaria, antidiabetic, wound-healing, hepato-protective, nerve-recovery, antiulcer, insecticidal, and anticancer effects have been observed in the latex. The research also found that excessive intake has negative health effects.

Conclusion
The review discovered that the biological evaluation of C. procera in vitro and in vivo animal models was well documented. Human safety and efficacy, however, have yet to be thoroughly tested, and additional well-designed clinical trials are required to confirm preclinical findings. It is essential to establish a standard dose and assure its safety.
The dose regimen formulation of doxycycline hydrochloride and florfenicol injection based on ex vivo pharmacokinetic-pharmacodynamic modeling against the Actinobacillus pleuropneumoniae in pigs
Authors: Yuan Yuanyuan , An Boyu , Xie Shuyu , Qu Wei , Hao Haihong , Huang Lingli , Luo Wanhe , Liang Jixiang , Peng Dapeng 
Published: February 2023

AbstractDoxycycline hydrochloride and florfenicol combination (DoxHcl&FF) is an effective treatment for respiratory diseases. In the study, our objective was to evaluate the activity of DoxHcl&FF against Actinobacillus pleuropneumoniae (APP) in porcine pulmonary epithelial lining fluid (PELF) and the optimal dosage scheme to avoid the development of resistance. The DoxHcl&FF was administered intramuscularly (IM) at 20 mg/kg, and the PELF was collected at different time points. The minimum inhibitory concentration (MIC) and time-mortality curves were also included in the study. Based on the sigmoid Emax equation and dose equations, the study integrated the in vivo pharmacokinetic data of infected pigs and ex vivo pharmacodynamic data to obtain the area under concentration time curve (AUC0-24h)/MIC values in PELF and achieve bacteriostatic activity, bactericidal activity and the virtual eradication of bacteria. The study showed that the combination of DoxHcl and FF caused no significant changes in PK parameters. The peak concentration (Cmax) of FF in healthy and diseased pigs was 8.87 ± 0.08 μg/mL and 8.67 ± 0.07 μg/mL, the AUC0-24h were 172.75 ± 2.52 h·μg/mL and 180.22 ± 3.13 h·μg/mL, the Cmax of DoxHcl was 7.91 ± 0.09 μg/mL and 7.99 ± 0.05 μg/mL, and the AUC0-24h was 129.96 ± 3.70 h·μg/mL and 169.82 ± 4.38 h·μg/mL. DoxHcl&FF showed strong concentration-dependent tendencies. The bacteriostatic, bactericidal, and elimination activity were calculated as 5.61, 18.83 and 32.68 h, and the doses were 1.37 (bacteriostatic), 4.59 (bactericidal) and 7.99 (elimination) mg/kg. These findings indicated that the calculated recommended dose could assist in achieving more precise administration, increasing the effectiveness of DoxHcl&FF treatment for APP infections.
The Impact of Dietary Cholesterol on Low-Density Lipoprotein: Lessons in Absorption and Overconsumption
Authors: Porter Michael , Wellons Melissa 
Published: February 2023

Abstract
This case describes a 58-year-old woman with past medical history of ulcerative colitis, hyperlipidemia, and radiological evidence of atherosclerosis without prior cardiovascular disease who presented for management of hyperlipidemia. At baseline, her lipid panel in 2015 noted a calculated low-density lipoprotein (LDL-C) of 125 mg/dL (3.2 mmol/L). Over the course of the next 5 years, she developed severe LDL elevations to &gt;400 mg/dL (&gt;10.3 mmol/L) following the addition of 1600 mg dietary cholesterol daily achieved through 9 eggs. Following cessation of this intake she had dramatic improvements in LDL, which was later further augmented significantly by initiation of ezetimibe. The impact of dietary cholesterol on lipid profiles has long been an area of controversy, and, for the average American, current guidelines do not recommend egg restriction as an effective tool for LDL lowering. However, as highlighted in this case, certain individuals may be more prone to high LDL when consuming high cholesterol diets. Further study on how to better identify these susceptible individuals could help improve nutritional and medication treatment plans for patients with dyslipidemia.
Metabolically Healthy Obesity is not a Myth
Authors: Farabi Sarah S , Smith Gordon I , Yoshino Jun , Klein Samuel 
Published: February 2023

Abstract
People with obesity who do not have the metabolic syndrome or components of the metabolic syndrome have been characterized as having metabolically healthy obesity (MHO). However, the existence of MHO has been questioned because people with MHO are at greater risk of developing diabetes and fatal cardiovascular disease than people who are lean and healthy. Here we report findings from a 25-year-old woman with rigorously defined MHO (normal oral glucose tolerance, insulin sensitivity [assessed using the hyperinsulinemic-euglycemic clamp procedure], plasma triglyceride, and intrahepatic triglyceride content) evaluated at baseline (body mass index, 37.7 kg/m2) and 5 years later, after a 32% (30.8 kg) increase in body mass (BMI, 49.6 kg/m2). Weight gain did not have adverse effects on fasting plasma glucose, oral glucose tolerance, β-cell function, insulin sensitivity, plasma triglyceride, intrahepatic triglyceride content, or carotid intima-media thickness. Adipose tissue expression of genes involved in extracellular matrix formation remained unchanged. Adipose tissue expression of several inflammation-related genes increased by more than 30%, but was not associated with a corresponding increase in plasma cytokine concentrations, with the exception of IL-6 and C-reactive protein. The present case study demonstrates that some people with obesity are resistant to the adverse cardiometabolic effects of excess adiposity and marked weight gain.
Significance of Pupillary Findings in Decision Making and Outcomes of World Federation of Neurological Societies Grade V Subarachnoid Hemorrhage
Authors: Kobata Hitoshi , Ikawa Fusao , Sato Akira , Kato Yoko , Sano Hirotoshi 
Published: February 2023

BACKGROUND:
There is considerable debate regarding the definition of grade V subarachnoid hemorrhage (SAH). Recently, the Glasgow Coma Scale-Pupil (GCS-P) score was advocated for assessing traumatic brain injury.

OBJECTIVE:
To study the significance of pupillary findings and GCS-P in the management and outcomes of the World Federation of Neurological Societies (WFNS) grade V SAH.

METHODS:
We analyzed data obtained from a Japanese nationwide prospective registration study on the modified WFNS SAH grading system. Bilateral pupillary dilatation without reactivity was defined as pupil score 2, unilateral pupil dilation without reactivity as score 1, and no pupil dilatation with reactivity as score 0. The GCS-P score was calculated by subtracting the pupil score from the total GCS score. The characteristics and pupillary findings were examined in patients with each GCS score in WFNS grade V.

RESULTS:
Among 1638 patients, 472 (men/women = 161/311, age 67.0 ± 14.0 years) had a GCS score of ≤6 after initial resuscitation on arrival. Overall, lower GCS scores were associated with poorer neurological outcomes and higher mortality. Aneurysms were treated in 20.5%, 53.9%, and 67.5% of patients with pupil scores of 2, 1, and 0, respectively. Favorable outcomes (modified Rankin Scale 0-2) at 3 months occurred in 4.5%, 10.8%, and 21.4% in patients with pupil scores 2, 1, and 0, respectively (P < .0001), and in 0%, 0%, 13.6%, 28.6%, 16.7%, and 18.8% of patients with GCS-P scores 1 to 6 (P < .0001), respectively.

CONCLUSION:
Our study confirmed the significance of pupillary findings in decision making and outcomes of WFNS grade V SAH.
Synthesis of α-Substituted Alkenylammonium Salts through Suzuki–Miyaura and Sonogashira Coupling
Authors: Yoshita Aoi , Sakakibara Yota , Murakami Kei 
Published: February 2023

Abstract
Alkenylammonium salts are a unique class of compounds, wherein an electron-withdrawing ammonio group is attached to an olefin. Although these salts are structurally simple, the synthesis of α-substituted alkenylammonium salts has been sparsely explored. Herein we report the synthesis of α-aryl and α-alkynylated alkenylammonium salts through Suzuki–Miyaura and Sonogashira coupling.
ON MOBILE LEARNING
Authors: Voicu Mirelacatrinel 
Published: February 2023

In this paper we are focused on emphasizing the importance of the mobile learning as the newest and most powerful way of learning. Today, mobile learning is possible due to many important IT domain players. For this reason, in the IT area, we accentuate the following subjects: the specifications of the actual mobile devices (smartphones and tablets), which can be used successfully in mobile learning; the 3G/4G generation of wireless mobile telecommunications technology and the most important providers of mobile Internet in Romania, their offers and prices; the cloud technologies which offer the storage support which is necessary for hosting such learning; the mobile websites, mobile applications and the most important technologies used to build them; the modern software technologies which can be used to provide content for mobile learning – here we present topics on Android Studio and Java programming, PhoneGap and JavaScript, CSS and HTML technologies; some of the most important providers of online learning and the way in which we can study their courses on mobile devices (we are focused on Oracle Academy, MongoDB University, Google, Udacity, Udemy, edX, Coursera, etc.); the difference between electronic formats of the lesson content: text files, video files, etc.; the greatest changes on learner lifestyle: on the one hand to learn more than ever before, on the other hand have more time for other activities. Finally, we present a scenario in which we can see how mobile learning can currently help the school education in Romania.
USING TECHNOLOGY TO TEACH CHEMISTRY. A THEORETICAL APPROACH
Authors: Ianos Maria gratiela , Oproiu Gabriela carmen 
Published: February 2023

The increasing tendency of digitalization, present in today's society, have an impact on education, as well. The benefits of using technology in the classroom have been mentioned in various studies. The internet offers many educational resources that can be used both offline and online and can be the proper instruments to support the instructional process. However, many studies on technology-assisted instruction highlight several factors that require a permanent restructuring and updating of contents, and also the adaptation of the applied didactic strategies. Among these factors there are: the particularities of new generations of students, the rapid evolution of technology, many existing web resources, the variety of instructional strategies, the diversity and complexity of studied disciplines etc. To facilitate the learning of various disciplines, and especially the basic scientific disciplines such as chemistry, it is necessary for teachers to be able to easily identify educational technology resources, and integrate them effectively in the didactic activity. Chemistry is an important discipline in preparing students and is one of the essential branches of science. Difficulties encountered by students in learning this discipline have been highlighted in various studies, and requires identifying the best strategies for teaching-learning of chemistry. Thus, the use of technology in teaching supports students in the learning process, can contribute to a better understanding of abstract concepts of chemistry and play an important role in reducing learning difficulties. Both theoretical and empirical research studies can provide useful information to practitioners and theorists in the field. The present paper represents a theoretical study on the implications of using technology in teaching chemistry. The study was performed by analysing of some scientific works, which aim at teaching chemistry assisted by technology. The main objective of the paper was to identify the most commonly used resources / tools and best practices for their use in teaching chemistry.
MULTIMEDIA AND DIGITAL TOOLS. BASICS OF CIVIC AND ETHICAL EDUCATION IN PRE-PRIMARY AND PRIMARY SCHOOL
Authors: Neacsu Mihaela gabriela , Bucuroiu Florentina 
Published: February 2023

This paper presents the findings of a psycho-pedagogical study on the effectiveness of certain multimedia apps and of a software program for civic and ethical education in pre-primary and primary school. The purpose of this study is both the analysis of the capitalization of various forms of multimedia, audio, image, animation, video content, interactive content forms and of the new educational software for shaping the social-civic skills of young pupils. The methodology employed is qualitative. The specific objectives of this study are:
?	An analysis of the pedagogical ways of integration of the multimedia and digital tools in the didactic process of civic and ethical education for young pupils;
?	An analysis of the complementarity of education forms and capitalisation of the multimedia and digital tools for shaping the social-civic skills of young pupils;
?	Best-practice reflections on integrating multimedia education means in Romanian pre-university education.
The conclusions of this study highlight the importance of integrating multimedia means in the civic and ethical education of young pupils so as to enable greater engagement of students in their own civic shaping, capitalisation of the differentiated and personalized content of civic education, a flexible structuring of the learning times, understanding civic reality based on case studies reflecting reality, the formation of pro-social attitudes and behaviours based on concrete examples. The advantages and limitations of using these formative tools and means are highlighted: multisensory stimulation in presenting the information, stimulation of critical thinking, purpose-oriented learning, individual exploration/search of information and operating on it, contributions to the knowledge of the immediate civic reality etc.
Biological hazards at the nurse's workplace
Authors: Tomaszewska Katarzyna , Kisiel Renata 
Published: February 2023

Occupational exposure to blood is a significant problem among health care workers. Nurses are a group that is particularly at risk. Exposure can lead to HBV, HCV or HIV infection. Extremely important for preventing exposures is improving knowledge of medical personnel about consequences of being infected by bloodborne pathogens, preventive measures and advantages of using those measures, and especially about post-exposure proceedings.
The aim of the study was to assess the knowledge and opinions of nurses employed in anesthesiology and intensive care wards on occupational exposure and post-exposure management.
Material and methods: A diagnostic survey method was employed for the research using author’s questionnaire. The study included 117 nurses working in the anesthesiology and intensive care units. Statistical analysis of the results was carried out using the descriptive statistics method. During the statistical verification p<0.05 was assumed as the level of significance of the obtained results.
Results and conclusions: The level of knowledge and opinions of the surveyed nurses were not dependent on sociodemographic factors. Nurses use personal protective equipment and know post-exposure procedure, but don’t always follow it. Training doesn’t reduce the number of exposure cases. The most common mechanism of exposure is needle-stick injury.
A PRODUÇÃO DE SOJA E SUA INFLUÊNCIA NAS VARIÁVEIS SOCIOECONÔMICAS DA REGIÃO DO MATOPIBA
Authors: Paula Nunes Ana , Gomes Campelo Filho Eulalio , Gustavo Benini Elcio 
Published: February 2023

O artigo centrou-se em analisar a evolução do agronegócio da soja nos municípios do Matopiba e sua correlação com os indicadores socioeconômicos da região. Trata-se de uma pesquisa explicativa, de abordagem quantitativa, tendo como amostra dados anuais de 160 municípios da região no período de 2004 à 2018. Por meio da regressão linear múltipla cruzou-se os dados de produção de grãos e os dados socioeconômicos dos municípios das regiões selecionadas. Com base nas variáveis escolhidas, os resultados indicaram uma correção significante para as quatro variáveis dependentes do estudo: Índice FIRJAN de Desenvolvimento Municipal Saúde; Emprego e Renda; Educação; Geral. Sinalizando um poder explicativo em torno de 30% da produção de grãos sobre os indicadores socioeconômicos dos municípios das microrregiões do Matopiba estudadas
RELACIONAMENTOS ENTRE MICROEMPREENDEDORES DE UM CONDOMÍNIO RESIDENCIAL EM MOMENTO DE COVID-19: uma análise sob o enfoque das redes sociais
Authors: DOS SANTOS AGNALDO ANTONIO , Ferreira João Cesar de Souza , FARINA MILTON CARLOS , CRISPIM SERGIO FELICIANO 
Published: February 2023

A Análise de Redes Sociais (ARS) pode ser aplicada como ferramenta para a compreensão das relações e conexões, se na rede há realização de trocas de negócios como ofertantes e compradores entre os integrantes do grupo de microempreendedores. Assim, o objetivo desta pesquisa é analisar a rede de interações de um grupo de microempreendedores de um condomínio residencial paulistano em tempos de COVID-19, com sustentação na abordagem teórica de análise de redes sociais. O artigo estuda a adoção de análise de redes sociais em negócios de microempreendedores, pouco explorados sobre o tema de forma integrada.
Projeto / metodologia / abordagem: Estudo descritivo e exploratório com análise em profundidade pelo uso de técnicas de análise de redes sociais, complementada pela análise de regressão simples.
Constatações: Verificou-se que o objetivo do estudo foi analisar a rede de interações de um grupo de microempreendedores de um condomínio residencial paulistano em tempos de Covid-19, com sustentação na abordagem teórica de análise de redes sociais. Conforme os dados obtidos, no grupo de microempreendedores é possível identificar que há um relacionamento muito forte entre os atores da rede, com poucos atores tipo hub, ampliando a troca de informações dentro da rede, como se esperava no objetivo deste artigo
Efek pemberian dosis akriflavin dan lama perendaman yang berbeda terhadap rasio pembentukan kelamin jantan ikan baung (Hemibagrus nemurus)
Authors: Akbar Junius , Hanafie Agussyarif 
Published: February 2023

Abstract. The purpose of this study was to examine the Acriflavine doses and immersion time on the sex ratio and survival rate of Bagrid catfish (Hemibagrus nemurus. The completely randomized design of factorial was applied in this research (4x2) with 3 replications. The tested doses were 0 mg/L, 2.5 mg/L, 5 mg/L, and 7.5 mg/L and immersion times were 6 hours and 12 hours. The Anova test showed that Acriflavine doses and immersion times were not influence significantly on survival rate and sex ratio of Hemibagrus nemurus. However, the highest male sex ratio was found 5 mg/L dose and 12 hours immersion time.Keywords : Bagrid catfish, Hemibagrus nemurus, acriflavine, masculinization Abstrak. Penelitian ini bertujuan untuk menguji pengaruh dosis akriflavin dan lama perendaman terhadap keberhasilan pembentukan kelamin jantan dan sintasan benih ikan baung yang terbaik. Rancangan yang digunakan adalah Rancangan Acak Lengkap Faktorial (4x2) dengan tiga kali ulangan. Variabel bebas yang diukur adalah kombinasi antara dosis akriflavin (0 mg/L, 2,5 mg/L, 5 mg/L, dan 7,5 mg/L) pada lama perendaman (6 jam dan 12 jam. Hasil uji Anova menunjukkan bahwa perlakuan dosis akriflavin dan lama perendaman tidak memberikan pengaruh yang nyata terhadap sintasan dan rasio kelamin ikan baung. Namun demikian hasil terbaik diperoleh pada perlakuan dosis 5 mg/L akriflavin dengan lama perendaman 12 jam, yakni 61,83%. Kata kunci : Baung, Hemibagrus nemurus, akriflavin, jantanisasi