bluuebunny/crossref_metadata_2025_split huggingface dataset:
Increased Maize Yield in Response to Applied Phosphorus and Nitrogen in the Greenbelt Zone, in South Sudan
Authors: Bazugba Isaac A. J. , Massawe Boniface H. , Shitindi Mawazo , Deng Pio K. 
Published: September 2023

Aims: The study focused on assessing the impact of applying phosphorus and nitrogen fertilizers on maize yield in the Greenbelt zone of South Sudan, where nutrient deficiencies often limit crop growth.
Study Design: Field experiments involved a 4 x 4 x 4 factorial experiments in a randomized complete block design. The field experiment was conducted in Sakure and Nginda Payams in Nzara and Yambio Counties respectively, between August 2020 and January 2022.
Methodology: The treatments involved Triple Super Phosphate (TSP, 0-46-0) applied at 20, 40 and 60 kg P ha -1 without N, Urea (46% N) applied at 40, 80 and 120 kg N ha-1 without P, combinations of each level of P with varying levels of N and an absolute control (P0N0). Maize variety was an open pollinated called NARD 1. Plot size was 16 m2, all maize plant above ground was harvested in a net plot (8.5 m2) after 120 days.
Results: The average maize yield in response to applied fertilizer P and N in the three sites was 4.7 t ha-1 an increase of about 62 % over the control (2.9 t ha-1), and was statistically significantly at P = .05
Conclusion: 20 kg P ha-1 and 120 kg N ha-1 rates were profitable and therefore were recommended as optimum application rates that can contribute to food security and economic progress in the area. However, more research is recommended to establish long-term fertilizer effects on maize performance, optimal application rates, and environmental considerations. The study stressed the need for diversified fertilizer research across seasons and emphasized community awareness about fertilizer benefits for sustainable maize production and farming system research.
Murine Models and Human Cell Line Models to Study Altered Dynamics of Ovarian Follicles in Polycystic Ovary Syndrome
Authors: Bevilacqua Arturo , Giuliani Cristiano , Emidio Giovanna Di , Myers Samuel H , Unfer Vittorio , Tatone Carla 
Published: January 2025

AbstractPolycystic ovary syndrome is one of the most common endocrine disorders in women of reproductive age, characterized by functional and structural alterations of the female reproductive organs. Due to the unknown underlying molecular mechanisms, in vivo murine models and in vitro human cellular models are developed to study the syndrome. These models are used to analyze various aspects of the pathology by replicating the conditions of the syndrome. Even though the complexity of polycystic ovary syndrome and the challenge of reproducing all its features leave several questions unanswered, studies conducted to date have elucidated some of the alterations in ovarian follicle molecular and cellular mechanisms involved in the syndrome, and do not require the employment of complex and invasive techniques on human patients. This review examines ovarian functions and their alterations in polycystic ovary syndrome, explores preclinical in vivo and in vitro models, and highlights emerging research and medical perspectives. It targets researchers, healthcare professionals, and academics, including endocrinologists, cell biologists, and reproductive medicine specialists, studying the molecular and cellular mechanisms of the syndrome.
Chromatin Marks H3K4me3 and H3K9me3 in Triple‐Negative Breast Cancer Cell Lines
Authors: Anjum Farhan , Kaushik Kush , Salam Abdul , Nandi Chayan Kanti 
Published: January 2025

AbstractTriple‐negative breast cancer (TNBC) is the most lethal and aggressive breast cancer among all the breast cancer subtypes. Despite several attempts, to date, there is an extensive lack of therapeutic intervention. Hence, there is a dire need for an effective biomarker to timely diagnose TNBC. Here, utilizing super‐resolution microscopy, the remodeling structural aspects of euchromatin and heterochromatin in TNBC are studied and the results are compared with non‐cancerous and non‐TNBC cell lines. The nanoscopic visualization reveals a distinct difference in chromatin remodeling in TNBC in comparison to the other two cell lines. While the euchromatin density is found to increase, the heterochromatin is found to decrease. A complete switching of the heterochromatin‐euchromatin ratio is observed in TNBC cells thus proposing that chromatin remodeling and chromatin morphological changes can be pursued as one of the targets for diagnostic purposes. Increased expression of structure specific recognition protein‐1(SSRP‐1) protein supports the increased rate of chromatin remodeling in breast cancer cell lines. The results may lead to developing a new strategy for diagnosing TNBC patients.
4D Printed Cardiac Occlusion Device with Efficient Anticoagulation, Proendothelialization, and Precise Localization
Authors: Yang Mengjiao , Xu Yingjie , Xin Xiaozhou , Zeng Chengjun , Li Yuanshi , Lin Cheng , Liu Yanju , Guo Yuanyuan , Leng Jinsong 
Published: January 2025

AbstractCongenital heart defects (CHDs) are one of the most common congenital malformations, accounting for ≈30% of all congenital malformations. Interventional implantation of occlusion devices is becoming the preferred treatment for CHDs. However, current occlusion devices suffer from serious problems, such as thrombosis, slow endothelialization, imprecise localization, abrasion, displacement, etc. Here, a multifunctional drug‐carrying fiber platform with structural similar to the extracellular matrix is innovatively designed to develop 4D printed cardiac occlusion devices, with characteristics of efficient anticoagulation, proendothelialization, and precise localization. Biomimetic ligament structures are designed to achieve a similar mechanical response to myocardial tissue, which helps to synergize deformation and reduce tissue wear. A structural design method for biomimetic personalized multilevel occlusion devices is proposed, facilitating further improvement of sealing reliability. The radiopaque 4D printed shape memory composites are developed, realizing the complete visualization and precise localization of the device in vivo. The novel 4D printed cardiac occlusion device provides an effective way to reduce the risk of complications and contributes to versatility. It is expected to be a next‐generation multifunctional repair device for personalized treatment of CHDs.
Hydrogel‐Based Photothermal‐Catalytic Membrane for Efficient Cogeneration of Freshwater and Hydrogen in Membrane Distillation System
Authors: Sun Jiawei , Farid Muhammad Usman , Xi Wenlong , Lu Gang , Boey Min Wei , Ravi Sai Kishore , Sit Patrick H.‐L. , An Alicia Kyoungjin 
Published: January 2025

AbstractPhotothermal‐catalytic (PTC) process is a promising way to produce freshwater and clean hydrogen by integrating solar‐driven desalination and water splitting. However, efficient solar spectrum utilization coupled with effective mass and thermal management poses key challenges in developing multifunctional PTC systems. Here, a highly stable hydrogel membrane (CdS/MX‐HM) that incorporates MXene and in‐situ generated CdS as the PTC materials into a customized vacuum membrane distillation (VMD) setup is demonstrated. The CdS/MXene composites utilize the full solar spectrum, with MXene contributing to photothermal ability and serving as a co‐catalyst to enhance photocatalytic performance, which are effectively integrated into the membrane system. These functional materials are immobilized within the hydrophilic PVA/chitosan hydrogel layer, which creates a supramolecular network that provides excellent stability and protection while offering an interface for PTC processes. Supported by the hydrophobic PTFE membrane substrate, the integrated system enables fast gas transfer to the permeate, completing the dual‐function design. Through systematic optimization of membrane structure and operational parameters, the PTC‐VMD system achieves a water flux of 1.63 kg m−2 h−¹ and a hydrogen production rate of 3099 µmol m−2 h−1 under one sun irradiation, demonstrating its potential as a sustainable solution for the water‐energy nexus challenge.
Metallic Sliding Ferroelectricity in Trilayer Penta‐NiN2
Authors: Hou Changsheng , Shen Yiheng , Wang Qian 
Published: January 2025

AbstractThe experimental observation of metallic ferroelectricity has challenged the traditional understanding in recent years, as metallicity and ferroelectricity are historically considered mutually exclusive. This breakthrough has sparked significant interest in this field. However, the coexistence of metallicity and sliding ferroelectricity in 2D pentagonal materials has not been reported yet. Here, this is theoretically studied that the sliding ferroelectricity in metallic trilayer penta‐NiN2. By scanning the configuration space of stacking patterns, a pair of polarized configurations is identified with strong out‐of‐plane ferroelectric polarization of ± 5.39 × 10−13 C m−1 and a low polarization switching barrier of 14.33 meV/atom together with a non‐polarized configuration as the intermediate state for the ferroelectric transition. It is also found that there are substantial changes in the second harmonic generation of the stable configurations that would facilitate experimental observation. This work demonstrates the potential of pentagonal sheets as promising metallic sliding ferroelectrics, which would significantly expand the family of sliding ferroelectric materials.
Polar Pore Surface of Polyamide Membranes Enabling Efficient Solvent Mixture Separation
Authors: Zhang Aiwen , Guan Kecheng , Mai Zhaohuan , Wang Zheng , Dai Liheng , Li Chuang , Li Bowen , Li Zhan , Hu Mengyang , Zhang Pengfei , Matsuyama Hideto 
Published: January 2025

AbstractSeparating solvent mixtures without phase change using polyamide membranes reduces energy consumption and enhances environmental sustainability. However, overemphasizing precise pore control while neglecting membrane–solvent interactions hinder membrane development and reduces separation efficiency. Here, it is demonstrated that rapid separation of solvents of differing polarity can be achieved using a polyamide membrane featuring relatively large pores with a polar surface formed via interfacial polymerization between polyethyleneimine (PEI) and trimesoyl chloride (TMC). The abundant amine groups and flexible chains of PEI facilitate the formation of a polyamide network that enables fast and selective transport of mixed solvents with varying polarity. The membrane can exhibit several‐fold‐higher permeance while maintaining comparable permselectivity compared to conventional polyamide membranes fabricated from the reaction of m‐phenylenediamine with TMC. This work leverages membrane–solvent interactions to achieve solvent mixture differentiation and may guide the development of high‐performance polymer membranes for efficient solvent mixture separation.
A Highly Bioactive Organic–Inorganic Nanoparticle for Activating Wnt10b Mediated Osteogenesis by Specifically Anchor CCN3 Protein
Authors: Qiu Yonghao , Wang Chunhui , Yang Yulian , Xu Shijing , Huang Haohui , Zhang Liuyang , Lei Bo , Zhao Fujian 
Published: January 2025

AbstractThe rapid and efficient bone regeneration is still in unsatisfactory outcomes, demonstrating alternative strategy and molecular mechanism is necessary. Nanoscale biomaterials have shown some promising results in enhancing bone regeneration, however, the detailed interaction mechanism between nanomaterial and cells/tissue formation is not clear. Herein, a molecular‐based inorganic–organic nanomaterial poly(citrate‐siloxane) (PCS) is reported which can rapidly enhance osteogenic differentiation and bone formation through a special interaction with the cellular surface communication network factor 3 (CCN3), further activating the Wnt10b/β‐catenin signaling pathway. Further studies revealed that the CCN3 is a key bridge protein for transmitting the osteoinductive effects of nano PCS into the intracellular compartment and activating Wnt10b. Specifically, the molecular mechanism studies confirmed that the inorganic silicon hydroxyl and the organic ester group can bound to the Thrombospondin‐1 (TSP‐1) and von Willebrand factor type C repeat module (vWC) structural domains of CCN3 respectively. The special material‐protein interaction induced a conformational change of CCN3 and activated the function of the TSP‐1 structural domain, which is further associated with the binding and activation of Wnt10b signaling. This study reveals the first targets of nanobiomaterials to promote tissue regeneration through cellular interactions and provides new ideas for the development of materiobiology.
An on‐Demand Oxygen Nano‐vehicle Sensitizing Protein and Nucleic Acid Drug Augment Immunotherapy
Authors: Zhang Sidi , Wang Xinghui , Chen Xiaojing , Shu Duohuo , Lin Quankun , Zou Hanbing , Dong Jialin , Wang Bing , Tang Qianyun , Li Huishan , Chen Xiaoxiang , Pu Jun , Gu Bin , Liu Peifeng 
Published: January 2025

AbstractHypoxia severely limits the antitumor immunotherapy for breast cancer. Although efforts to alleviate tumor hypoxia and drug delivery using diverse nanostructures achieve promising results, the creation of a versatile controllable oxygen‐releasing nano‐platform for co‐delivery with immunostimulatory molecules remains a persistent challenge. To address this problem, a versatile oxygen controllable releasing vehicle PFOB@F127@PDA (PFPNPs) is developed, which effectively co‐delivered either protein drug lactate oxidase (LOX) or nucleic acids drug unmethylated cytosine‐phosphate‐guanine oligonucleotide (CpG ODNs). Upon photothermal heating, this platform triggered oxygen release, thereby augmenting LOX‐mediated lactate detection rates, and improving T cells infiltrating and cytokine expression. Moreover, under an oxygenated tumor microenvironment (TME), PFPNPs co‐delivered with CpG ODNs effectively reprogrammed the immunosuppressive TME by repolarizing macrophages to an M1‐like phenotype, promoting dendritic cells maturation, and increasing tumor‐infiltrating T cells while decreasing the ratio of regulatory T cells (Tregs). Our study demonstrated that this controlled oxygen‐releasing platform possessed adaptive drug‐loading capabilities to meet varied immunotherapeutic demands in clinical settings.
10 Years Development of Potassium‐Ion Batteries
Authors: Li Mingnan , Wang Caoyu , Wang Cheng , Lyu Yanqiu , Wang Jingxiu , Xia Shuixin , Mao Jianfeng , Guo Zaiping 
Published: January 2025

AbstractPotassium‐ion batteries (PIBs), with abundant resources and low cost, are considered as a promising alternative to commercial lithium‐ion batteries for low‐cost and large‐scale applications. Over the past decade, significant academic progresses are made in the development of PIBs, including advancements in cathodes, anodes, and electrolytes. However, most improvements are achieved under laboratory conditions (e.g., K metal‐based half‐cells and low mass loading of active materials), and the performance of PIBs in full cells is still far from the requirements for commercial applications. A critical insight bridging the academic research and the commercialization of PIBs is urgently needed to guide future research in this field. This review will discuss the challenges and improvement strategies in the development of PIBs, focusing on the potential practical cathodes, anodes, and electrolytes, as well as their performance in full cells. It aims to give the readers a clear and logical understanding of the development of PIBs. The application analysis is also discussed to provide a comprehensive understanding of the commercialization potential of PIBs. Finally, perspectives are provided for the future development of PIBs.
Single‐Cell Quantification of Viscoelastic Phase Transitions in 3D Tissues
Authors: Tomizawa Yuji , Wali Khadija H. , Surti Manav , Suhail Yasir , Kshitiz  , Hoshino Kazunori 
Published: January 2025

AbstractTransitions of biological tissues between solid‐like and liquid‐like phases have been of great recent interest. Here, the first successful cell‐by‐cell evaluation of tissue viscoelastic transition is presented. An in situ micro‐mechanical perturbation is applied to a microtissue, and the resulting volumetric deformation is evaluated using 3D light‐sheet microscopy and digital image correlation (DIC), quantifying both solid‐like, well‐aligned displacement and liquid‐like swirling motion between individual cells. The viscoelastic transition of fibroblasts is crucial in fundamental physiological events, such as placentation, cancer dissemination, and wound healing. This study investigates 3D organoid systems modeling maternal‐fetal and tumor‐stroma interfaces, demonstrating established molecular and structural parallels. The analysis visualizes individual cells in stromal‐epithelial interactions and how they collectively alter tissue viscoelastic properties. It also enables in‐silico microdissection, linking single‐cell viscoelasticity with multi‐channel fluorescence. RNAseq analysis of endometrial stromal fibroblasts shows that decidualization activates mechano‐transcriptional regulators, including myocardin‐related transcription factors (MRTFs), associated with increased cellular contractility and actomyosin mobilization. Knocking down MRTFA in cancer‐associated fibroblasts in the tumor‐fibroblast co‐culture 3D model induces significant changes in fibroblast properties, mirroring those observed in the maternal‐fetal interface model, highlighting parallels between placentation and cancer invasion. This analysis confirms existing beliefs and discovers new insights broadly applicable to studying organoids, embryos, tumors, and other tissues.
Er3+‐Tm3+ Co‐Doped Hybridized Gadolinium Aluminosilicate Glass Fiber for Broadband Optical Amplification
Authors: Yu Zhuoming , Huang Yupeng , Han Yi , Liu Ziang , Chen Jingfei , Feng Xu , Li Xueliang , Zhou Shifeng 
Published: January 2025

AbstractOptical fiber communication has greatly promoted the development of the information age, while the emergence of 5G networks, cloud computing, and artificial intelligence have put forward high challenges to the capacity of current optical fiber communication systems. At present, increasing the bandwidth of erbium‐doped fiber amplifiers (EDFA) is the most effective way to increase the communication capacity. In this paper, a hybridization strategy is proposed for simultaneous achieving strong and flat optical response and demonstrate the success in construction of Er3+‐Tm3+ co‐doped gadolinium aluminosilicate glass fiber for L‐band optical amplification. Gd3+ ions are introduced to enhance the radiative transition by improving the dispersibility of Er3+. Tm3+ ions are co‐introduced to facilitate energy transfer between Er3+ and Tm3+ for achieving flat emission in the L‐band. The hybridized active fiber which can be effectively fused with quartz fiber is fabricated by melt‐in‐tube (MIT) approach. A fiber amplifier is construed and it enables to achieve a flat on‐off gain (<±0.76 dB) across the L‐band spectrum. These results indicate that Er3+‐Tm3+ co‐doped hybridized gadolinium aluminosilicate glass fiber is a promising gain material for fiber amplifiers and demonstrate that the hybridization approach provides a new strategy for the development of novel active fiber device.
Boosting Efficiency in Flexible Perovskite Solar Cells with Novel HTLs and ETLs: A drift‐diffusion numerical study of CBz‐PAI Interlayers and MXene Back Contacts
Authors: Benatallah Mohamed Amine , Elmohri Abdennour , Bouderbala Yaacoub Ibrahim , Alam Mir Waqas , Rabhi Selma 
Published: January 2025

AbstractIn this study, the functioning of flexible perovskite solar cells (FPSCs) is examined using drift‐diffusion SCAPS‐1D simulations under ideal conditions. The focus is on the CBz‐PAI interlayer at the perovskite and hole transport layer (HTL) interface and the impact of innovative materials for HTLs, electrons transport layers (ETLs), and transparent conduction electrodes (TCOs), such as AZO and MXene, in the front and back contacts. Initially, 50 configurations of ETLs, including BaZrS3, SnS2, STO, WS2, and ZrS2, as well as HTLs such as ACZTSe, CuBiS3, CZGS, and D‐PBTTT‐14, are tested to identify optimal architecture for enhancing device efficiency. The incorporation of the CBz‐PAI interlayer effectively reduces interfacial charge recombination, minimizing VOC losses and boosting overall performance. After further optimization and the integration of MXene as a back contact, the final FPSC design (PET/ITO/AZO/ZrS2/(FAPbI3)0.77(MAPbBr3)0.14(CsPbI3)0.09/CBz‐PAI/CZGS/MXene‐V3C2F2) achieves an impressive PCE of 27.17%, setting a new benchmark for FPSC efficiency.
3D‐Printed Myocardium‐Specific Structure Enhances Maturation and Therapeutic Efficacy of Engineered Heart Tissue in Myocardial Infarction
Authors: Wu Yong , Wang Yaning , Xiao Miao , Zhang Guangming , Zhang Feixiang , Tang Mingliang , Lei Wei , Jiang Ziyun , Li Xiaoyun , Zhang Huiqi , Ren Xiaoyi , Xu Yue , Zhao Xiaotong , Guo Chenxu , Lan Hongbo , Shen Zhenya , Zhang Jianyi , Hu Shijun 
Published: January 2025

AbstractDespite advancements in engineered heart tissue (EHT), challenges persist in achieving accurate dimensional accuracy of scaffolds and maturing human induced pluripotent stem cell‐derived cardiomyocytes (hiPSC‐CMs), a primary source of functional cardiac cells. Drawing inspiration from cardiac muscle fiber arrangement, a three‐dimensional (3D)‐printed multi‐layered microporous polycaprolactone (PCL) scaffold is created with interlayer angles set at 45° to replicate the precise structure of native cardiac tissue. Compared with the control group and 90° PCL scaffolds, the 45° PCL scaffolds exhibited superior biocompatibility for cell culture and improved hiPSC‐CM maturation in calcium handling. RNA sequencing demonstrated that the 45° PCL scaffold promotes the mature phenotype in hiPSC‐CMs by upregulating ion channel genes. Using the 45° PCL scaffold, a multi‐cellular EHT is successfully constructed, incorporating human cardiomyocytes, endothelial cells, and mesenchymal stem cells. These complex EHTs significantly enhanced hiPSC‐CM engraftment in vivo, attenuated ventricular remodeling, and improved cardiac function in mouse myocardial infarction. In summary, the myocardium‐specific structured EHT developed in this study represents a promising advancement in cardiovascular regenerative medicine.
Two Steps Li Ion Storage Mechanism in Ruddlesden–Popper Li2La2Ti3O10
Authors: Jang Mi , Hwang Sunhyun , Chae Ji Su , Jang Gun , Park Ho Seok , Lee Yunki , Choi JungHyun , Yoon Won‐Sub , Roh Kwang Chul 
Published: January 2025

AbstractInnovative anode materials are essential for achieving high‐energy‐density lithium‐ion batteries (LIBs) with longer lifetimes. Thus far, only a few studies have explored the use of layered perovskite structures as LIB anode materials. In this study, the study demonstrates the performance and charge/discharge mechanism of the previously undefined Ruddlesden‐Popper Li₂La₂Ti₃O₁₀ (RPLLTO) as an anode material for LIBs. RPLLTO exhibits two unique voltage plateaus ≈0.6 and 0.4 V(vs Li/Li+), due to the insertion of lithium ions into different sites within its layered structure. The electrical state of Ti is analyzed using X‐ray photoelectron spectroscopy and X‐ray absorption near edge spectra, revealing a reduction from Ti⁴⁺ to Ti2⁺, corresponding to a capacity of 170 mAh·g⁻¹. In situ X‐ray diffraction patterns and extended X‐ray absorption fine structure spectra demonstrate the crystal structure changes during lithiation. Complementary expansion along the a/b axes and contraction along the c axis result in a volume change of only 4%. This structural stability is evidenced by an 88% capacity retention after 1000 cycles. This study successfully showcases the lithium‐ion storage capability of RPLLTO and contributes to the development of perovskite anode materials with diverse compositions and structures.