bluuebunny/crossref_metadata_2025_split huggingface dataset:
Prognostic prediction based on histopathologic features of tumor microenvironment in colorectal cancer
Authors: Shi Liang , Zhang Yuhao , Wang Hong 
Published: April 2023

PurposeTo automatically quantify colorectal tumor microenvironment (TME) in hematoxylin and eosin stained whole slide images (WSIs), and to develop a TME signature for prognostic prediction in colorectal cancer (CRC).MethodsA deep learning model based on VGG19 architecture and transfer learning strategy was trained to recognize nine different tissue types in whole slide images of patients with CRC. Seven of the nine tissue types were defined as TME components besides background and debris. Then 13 TME features were calculated based on the areas of TME components. A total of 562 patients with gene expression data, survival information and WSIs were collected from The Cancer Genome Atlas project for further analysis. A TME signature for prognostic prediction was developed and validated using Cox regression method. A prognostic prediction model combined the TME signature and clinical variables was also established. At last, gene-set enrichment analysis was performed to identify the significant TME signature associated pathways by querying Gene Ontology database and Kyoto Encyclopedia of Genes and Genomes database.ResultsThe deep learning model achieved an accuracy of 94.2% for tissue type recognition. The developed TME signature was found significantly associated to progression-free survival. The clinical combined model achieved a concordance index of 0.714. Gene-set enrichment analysis revealed the TME signature associated genes were enriched in neuroactive ligand-receptor interaction pathway.ConclusionThe TME signature was proved to be a prognostic factor and the associated biologic pathways would be beneficial to a better understanding of TME in CRC patients.
Shenkang injection combined with alprostadil for chronic renal failure: A systematic review and meta-analysis
Authors: Xie Feng , Zhang Tiantian , Zhang Pu , Qu Xinliang , Li Min , Lan Wei 
Published: April 2023

ObjectiveTo systematically evaluate the clinical efficacy and safety of Shenkang injection (SKI) combined with alprostadil in the treatment of chronic renal failure (CRF).MethodRandomized controlled trials (RCTs) of Shenkang injection combined with alprostadil in CRF treatment were investigated by retrieving a total of 7 databases including CNKI, Wanfang database, VIP, CBM, PubMed, Embase and Cochrane Library, with the search time ranging from 2012 to now. Revman 5.2 software was used for data analysis, and Cochrane bias risk tool was used to evaluate the quality of the included literature. The final results were represented by relative risk (RR), mean difference (MD) and 95% confidence interval (95% CI).ResultsA total of 20 RCTs and 1,573 patients were included in this study. Meta-analysis showed that the overall response rate (ORR) of the treatment group was superior to the control group [RR = 0.20, 95% CI (0.16, 0.25), P &lt; 0.00001]. Compared with the control group, the treatment group achieved favorable improvement in terms of the creatinine clearance rate (Ccr) [MD = 9.48, 95% CI (8.73, 10.24), P &lt; 0.00001], serum creatinine (Scr) [MD = −55.12, 95% CI (−63.42, −46.82), P &lt; 0.00001], quantitative urine protein (Upro) [MD = −0.48, 95% CI (−0.53, −0.43), P &lt; 0.00001], and blood urea nitrogen (BUN) [MD=-3.73, 95% CI (−4.08, −3.3) 7, P &lt; 0.00001]. There was no statistical difference in the incidence of adverse reactions in each group.ConclusionCurrently, Shenkang injection combined with alprostadil has been widely used in clinical treatment of CRF due to the certain effect superior to other methods. However, its specific efficacy and safety need to be further verified through numerous large-scale clinical trials.
Predictive role of blood-based indicators in neuromyelitis optica spectrum disorders
Authors: Fang Xiqin , Sun Sujuan , Yang Tingting , Liu Xuewu 
Published: April 2023

IntroductionThis study aimed to assess the predictive role of blood markers in neuromyelitis optica spectrum disorders (NMOSD).MethodsData from patients with NMOSD, multiple sclerosis (MS), and healthy individuals were retrospectively collected in a 1:1:1 ratio. The expanded disability status scale (EDSS) score was used to assess the severity of the NMOSD upon admission. Receiver operating characteristic (ROC) curve analysis was used to distinguish NMOSD patients from healthy individuals, and active NMOSD from remitting NMOSD patients. Binary logistic regression analysis was used to evaluate risk factors that could be used to predict disease recurrence. Finally, Wilcoxon signed-rank test or matched-sample t-test was used to analyze the differences between the indicators in the remission and active phases in the same NMOSD patient.ResultsAmong the 54 NMOSD patients, neutrophil count, neutrophil-to-lymphocyte ratio (NLR), platelet-to-lymphocyte ratio (PLR), and systemic immune-inflammation index (SII) (platelet × NLR) were significantly higher than those of MS patients and healthy individuals and positively correlated with the EDSS score of NMOSD patients at admission. PLR can be used to simultaneously distinguish between NMOSD patients in the active and remission phase. Eleven (20.4%) of the 54 patients had recurrence within 12 months. We found that monocyte-to-lymphocyte ratio (MLR) (AUC = 0.76, cut-off value = 0.34) could effectively predict NMOSD recurrence. Binary logistic regression analysis showed that a higher MLR at first admission was the only risk factor for recurrence (p = 0.027; OR = 1.173; 95% CI = 1.018–1.351). In patients in the relapsing phase, no significant changes in monocyte and lymphocyte count was observed from the first admission, whereas patients in remission had significantly higher levels than when they were first admitted.ConclusionHigh PLR is a characteristic marker of active NMOSD, while high MLR is a risk factor for disease recurrence. These inexpensive indicators should be widely used in the diagnosis, prognosis, and judgment of treatment efficacy in NMOSD.
Mass spectrometry-based multimodal approaches for the identification and quantification analysis of microplastics in food matrix
Authors: Wu Pengfei , Wu Xiaoyi , Huang Qing , Yu Qinwei , Jin Hangbiao , Zhu Minghai 
Published: April 2023

BackgroundMicroplastics (MPs) and nanoplastics (NPs) have become emerging contaminants worldwide in food matrices. However, analytical approaches for their determination have yet to be standardized. Therefore, a systematic study is urgently needed to highlight the merits of mass spectrometry (MS) based methods for these applications.PurposeThe aim of the study is to review the current status of MS-based multimodal analysis for the determination of MPs in food matrices.MethodsWeb of Science and Google Scholar databases were searched and screened until Jan. 2023. Inclusion criteria: “publication years” was set to the last decades, “English” was selected as the “language,” and “research area” was set to environmental chemistry, food analysis and polymer science. The keywords were “microplastics,” “nanoplastics,” “determination,” “identification/quantification,” and “mass spectrometry.”ResultsTraditional spectrometry techniques offer good abilities to conduct the multimodal analysis of MPs in terms of color, shape and other morphologies. However, such technologies have some limitations, in particular the relatively high limits of detection. In contrast, MS-based methods supply excellent supplements. In MS-based methods, gas chromatographic-mass spectrometry (GC-MS), and LC-MS/MS were selected as representative methods for determining MPs in the food matrices, while specialized MS methods (i.e., MALDI-ToF MS and ToF-SIMS) were considered to offer great potential in multimodal analysis of MPs especially when interfaced with the imaging systems.SignificanceThis study will contribute to gaining a deeper insight into the assessment of the exposure levels of MPs in human body, and may help build a bridge between the monitoring studies and the toxicology field.
GRN is a prognostic biomarker and correlated with immune infiltration in glioma: A study based on TCGA data
Authors: Xu Su-Mei , Xiao Hai-Yan , Hu Zhong-Xu , Zhong Xue-Feng , Zeng You-Jie , Wu You-Xuan , Li Dai , Song Tao 
Published: April 2023

BackgroundAmong primary brain tumors, gliomas are associated with a poor prognosis and a median survival that varies depending on the tumor grade and subtype. As the most malignant form of glioma, glioblastoma (GBM) constitutes a significant health concern. Alteration in granulin(GRN) has been proved to be accountable for several diseases. However, the relationship between GRN and GBM remains unclear. We evaluated the role of GRN in GBM through The Cancer Genome Atlas (TCGA) databaseMethodsFirst, we assessed the relationship between GRN and GBM through the GEPIA database. Next, the relationship between GRN and GBM prognosis was analyzed by logistic regression and multivariate cox methods. Using CIBERSORT and the GEPIA correlation module, we also investigated the link between GRN and immune infiltrates in cancer. Using the TCGA data, a gene set enrichment analysis (GSEA) was performed. We also employed Tumor Immune Estimation Resource (TIMER) to examine the data set of GRN expression and immune infiltration level in GBM and investigate the cumulative survival in GBM. We also validated tissues from GBM patients by Western blotting, RT-qPCR, and immunohistochemistry.ResultsIncreased GRN expression was shown to have a significant relationship to tumor grade in a univariate study utilizing logistic regression. Furthermore, multivariate analysis disclosed that GRN expression down-regulation is an independent predictive factor for a favorable outcome. GRN expression level positively correlates with the number of CD4+ T cells, neutrophils, macrophages, and dendritic cells (DCs) that infiltrate a GBM. The GSEA also found that the high GRN expression phenotype pathway was enriched for genes involved in immune response molecular mediator production, lymphocyte-mediated immunity, cytokine-mediated signaling pathway, leukocyte proliferation, cell chemotaxis, and CD4+ alpha beta T cell activation. Differentially enriched pathways in the Kyoto Encyclopedia of Genes and Genomes (KEGG) include lysosome, apoptosis, primary immunodeficiency, chemokine signaling pathway, natural killer cell-mediated cytotoxicity, and B cell receptor signaling pathway. Validated result showed that GRN was upregulated in GBM tissues. These results suggested that GRN was a potential indicator for the status of GBM.ConclusionGRN is a prognostic biomarker and correlated with immune infiltrates in GBM.
Evaluation of the drug-drug interaction potential of the novel hepatitis B and D virus entry inhibitor bulevirtide at OATP1B in healthy volunteers
Authors: Zhu Vanessa , Burhenne Jürgen , Weiss Johanna , Haag Mathias , Hofmann Ute , Schwab Matthias , Urban Stephan , Mikus Gerd , Czock David , Haefeli Walter E. , Blank Antje 
Published: April 2023

Introduction: Bulevirtide is a first-in-class antiviral drug to treat chronic hepatitis B/D. We investigated the drug-drug interaction potential and pharmacokinetics of high-dose subcutaneous bulevirtide (5 mg twice daily) with organic anion transporting polypeptide 1B1 (OATP1B1) and cytochrome P450 (CYP) 3A4.Methods: This was a single-center, open-label, fixed-sequence drug-drug interaction trial in 19 healthy volunteers. Before and at bulevirtide steady state, participants ingested a single 40 mg dose of pravastatin. A midazolam microdose was applied to quantify CYP3A4 activity.Results: At bulevirtide steady state, pravastatin area under the concentration-time curve (AUC0–∞) increased 1.32-fold (90% CI 1.08-1.61). The 5 mg bulevirtide twice-daily treatment resulted in a mean AUC0-12 of 1210 h*ng/ml (95% CI 1040-1408) and remained essentially unchanged under the influence of pravastatin. CYP3A4 activity did not change to a clinically relevant extent. As expected, total bile acids increased substantially (35-fold) compared to baseline during bulevirtide treatment. All study medication was well tolerated.Discussion: The study demonstrated that high-dose bulevirtide inhibited OATP1B-mediated hepatic uptake of the marker substrate pravastatin but the extent is considered clinically not relevant. Changes in CYP3A4 activity were also not clinically relevant. In conclusion, this study suggests that OATP1B substrate drugs as well as CYP3A4 substrates may safely be used without dose adjustment in patients treated with bulevirtide. However, in patients using high statin doses and where concomitant factors potentially further increase statin exposure, caution may be required when using bulevirtide.
Impact of glucagon-like peptide 1 receptor agonist liraglutide and dipeptidyl peptidase-4 inhibitor sitagliptin on bowel cleaning and gastrointestinal symptoms in type 2 diabetes
Authors: Tong Yan , Huang Jian Qing , Chen Yang , Tu Mei , Wang Wei 
Published: April 2023

Objective: Glucagon-like peptide 1 receptor agonists (GLP-1 RAs) and dipeptidyl peptidase-4 inhibitors (DPP-4i) profoundly affect the gastrointestinal motor system, which may increase the incidence of inadequate bowel cleaning and gastrointestinal symptoms. Hence, this observational study mainly aimed to assess the influence of GLP-1 RAs liraglutide and DPP-4i sitagliptin on bowel preparation in type 2 diabetes (T2DM).Method: This observational study consecutively enrolled T2DM scheduled for a colonoscopy. Participants were prospectively separated into the liraglutide group (n = 120), sitagliptin group (n = 120), and control group (n = 120) based on the current hypoglycemic regimen. 3L split-dose polyethylene glycol regimens were used for bowel preparation. Experienced gastrointestinal endoscopists conducted colonoscopies. Lawrance Bowel-Preparation Tolerability Questionnaire and Boston Bowel Preparation Scale (BBPS) were conducted to assess bowel cleaning quality, tolerability, and safety.Results: The incidence of inadequate bowel cleaning was 17.5% in the liraglutide group, 20.5% in the sitagliptin group, and 21.7% in the control group. The difference among the three groups was not statistically significant (p = 0.927). Meanwhile, there were no significant differences in the mean BBPS, cecal intubation time, and polyp-detecting rates among the three groups (all p &gt; 0.0.05). Nausea, vomiting, and bloating scores were increased in the liraglutide group compared with the other two groups (p &lt; 0.05), whereas most were mild or very mild. Subgroup analyses showed that the incidence of inadequate bowel cleaning in T2DM with diabetic peripheral neuropathy (DPN) was increased in the liraglutide group compared with the sitagliptin group (61.3% vs. 32.1%, p = 0.022) and control group (61.3% vs. 32.8%, p = 0.025).Conclusion: GLP-1RA liraglutide or DPP-4i sitagliptin did not significantly increase the incidence of inadequate bowel cleaning and gastrointestinal symptoms during bowel preparation. Liraglutide may increase the incidence of inadequate bowel preparation in patients with DPN. This study reveal that more attention and aggressive bowel preparation regimens should be given to the T2DM with DPN.Clinical Trial Registration: (https://www.chictr.org.cn/index.aspx), identifier (ChiCTR2200056148).
Dynamic modal characteristics of transverse mode instabilities in ytterbium-doped fiber laser oscillator
Authors: Chai Junyu , Liu Wenguang , Wen Yujun , Wang Xiaolin , Xie Kun , Zhou Qiong , Zhang Hanwei , Zhang Jiangbin , Liu Pengfei , Zhang Dan , Lu Yao , Jiang Zongfu , Zhao Guomin 
Published: April 2023

In recent years, transverse mode instability (TMI) has been widely observed in fiber laser amplifier systems. The transverse mode instability phenomenon in fiber laser oscillators is less studied. Here, we focus on the dynamical output properties, i.e., its temporal signal and modal characteristics in a 30-μm-core-diameter ytterbium (Yb)-doped fiber laser oscillator. The TMI occurs at a pumping power around 310 W. Different from amplifiers, the basic oscillation frequency is quite low, at around 100 Hz, changing with time and pump power. When the fiber laser oscillator operates beyond TMI threshold at 357 W or 377 W for a while, the temporal fluctuation slowly disappears together with a decreased oscillation frequency, and appears again later. Based on the mode decomposition technique, we find that during the period of fluctuation disappearance at 357 W, the power output stays low and the output beam is still a mixture of fundamental mode and higher-order modes. The fundamental mode content is calculated to be averagely higher when temporal fluctuation disappears, increasing from ∼57% to ∼63%. Our results indicate complex interaction between the fiber laser oscillation and the TMI effect, and calls for more attention into understanding TMI in fiber laser oscillators.
Two years COVID-19 pandemic: Development of university students' mental health 2020–2022
Authors: Kohls Elisabeth , Guenthner Lukas , Baldofski Sabrina , Brock Tanja , Schuhr Jan , Rummel-Kluge Christine 
Published: April 2023

BackgroundThe literature indicates a negative impact on the mental health of university students during the COVID-19 pandemic. It remains unclear if this negative impact persists even after lockdown measures are lifted. The current study therefore investigates the mental health status of students by drawing on two previous studies the present study seeks to investigate differences in the mental health status across three time points.MethodsA cross-sectional, anonymous online survey among students of six universities was conducted between April and May 2022 (N = 5,510). Symptoms of depression, anxiety, hazardous alcohol use and eating disorders as well as social and emotional variables were assessed utilizing standardized instruments. Risk- and protective factors for severity of depressive and anxiety symptoms were investigated using multiple regression models. Differences in e.g., symptoms of depression across three time points were assessed with one-way analysis of variance.ResultsMore than one third of students exhibited clinically relevant symptoms of depression (35.5%), hazardous alcohol use (33.0–35.5% depending on gender) or anxiety disorder (31.1%). Taken together, almost two out of three (61.4%) students reported clinically relevant symptoms in at least one of the aforementioned symptom patterns, while almost one fifth of students reported suicidal ideation or thoughts of self-harm (19.6%). Higher perceived stress and loneliness significantly predicted higher levels of depressive symptoms, while resilience and social support were identified as protective factors. Compared to 2020 and 2021, levels of depressive symptoms were significantly reduced in 2022, levels of hazardous alcohol consumption showed a small but significant increase from 2021 to 2022. Worryingly, prevalence of suicidal ideation was the highest yet, being significantly higher than in 2020 (14.5%) and 2021 (16.5%).ConclusionThese results confirm previous results that the pandemic had and still has a negative impact on the mental health of university students. The present study broadens this view by the fact that some areas seem to recover quicker, while others seem to increase worryingly. Especially the persistent rise in suicidal ideation from 2020 to 2021 and to 2022, a constant reduction in reported social support and associated perceived loneliness is concerning. The claim for low-threshold and accessible mental health support for university students remains the same as in the beginning of the pandemic.
Current situation and influencing factors for suicidal intent in patients with intentional acute pesticide poisoning
Authors: Ma Shuang , Wen Zixin , Sun Long , Zheng Yingying , Zhang Yanxia , Shi Longke , Li Yaqian , Yu Guangcai , Zhang Jie , Kan Baotian , Jian Xiangdong 
Published: April 2023

IntroductionSince pesticides have been widely used in agricultural production, acute pesticide poisoning (APP) has gradually become a worldwide public health problem. Recently, the number of APP cases has been high in China, and the intentional self-administration of pesticides is the main cause of APP. However, there is a lack of relevant studies on the factors influencing suicidal intent in patients with intentional APP. This study aimed to explore the current situation and influencing factors for suicidal intent among patients with intentional APP.MethodsIn this cross-sectional study, we enrolled a total of 225 patients with intentional APP admitted to the emergency department of our Grade A comprehensive hospital in Shandong Province between June 2019 and January 2021. Patients were investigated using a health status interview questionnaire, Beck Suicidal Intent Scale, Duke Social Support Index, psychological stress scale, Dickman Impulsivity Inventory, Trait Anxiety Inventory, Center for Epidemiologic Studies Depression Scale, and Beck Hopelessness Scale. Descriptive statistics, single-factor analysis, and multiple linear regression were used for data analysis.ResultsSuicidal intent scores were collected and averaged (14.23 ± 6.22). Multiple linear regression analysis revealed that marital status, residential area, impulsivity, hopelessness, depression, psychological strain, and social support impact suicidal intent.ConclusionPatients with intentional APP have high suicidal intent. Therefore, different interventions should be tailored to different patients.
Approche projective des traits de la relation d'objet allergique et de l'organisation allergique essentielle
Authors: Pheulpin Marie-Christine 
Published: April 2023

Marie-Christine, Projective approach of the characteristics of allergic object relations and of the essential allergic organization.
The in-depth psychological investigation of subjects hospitalized for serious acute asthma has enabled us to identify patients with characteristics of the allergic object relation. We discovered the specificities of their Rorschach and TAT by outlining the main lines of expression. Their analysis helps to get a more nuanced diagnostic assessment of the personality organization and sometimes leads to the identification of a less-known organization : the essential allergic organization.

Key-words : Essential allergic organization, Rorschach-TAT, Mental funtionning, 8th month anxiety, Borderling problematic.
L'inhibition intellectuelle à la pré-adolescence : mise en défaut de la latence et prélude à la séparation
Authors: Emmanuelli Michèle 
Published: April 2023

Michèle Emmanuelli, La inhibición intelectual en la pre-adolescencia : puesta en falla de la latencia y preludio a la separación.
La distancia aportada por la latencia fiente a la situación edípica permite el funcionamiento no conflictualizado de las actividades intelectuales investidas sobre el modo fálico ; ella autoriza una repartición libidinal diferente, una investidura narcisística del pensamiento. La inhibición intelectual sobreviniente en la pre-adolescencia aparece como una puesta en falla del trabajo de la latencia. La problemática edípica esta en el centra de las dificultades de pensamiento, en su doble vertiente pulsional, sexual y agresiva, pero también del hecho de la repercusión narcisística que ella opera. La proximidad del trabajo de separación que la adolescencia va a exigir juega también un roi inhibidor. Las pruebas proyeetivas permiten el acceso a estos diferentes puntos.

Palabras-clave : Pre-adolescencia, Proceso de pensamiento, Inhibición intelectual, Fracaso escolar, Rorschach, TAT, Narcisismo, Edipo, Período de latencia.
Approche psychodynamique de la mort subite du nourrisson
Authors: Touvenot Valérie 
Published: April 2023

Valérie Touvenot, A psychodynamic approach to SIDS : Sudden Infant Death Syndrome.
The author has undertaken a critical review of psychodynamic positions (psychoanalytic, psychosomatic and haptonomie points of view) developped to explain Sudden Infant Death Syndrome (SIDS). A research methodology is proposed to test the different hypotheses at an empirical level.

Key words : Sudden Infant Death Syndrome, Psychoanalysis, Psychosomatic, Haptonomy, Critical review.