bluuebunny/crossref_metadata_2025_split huggingface dataset:
Infection-induced vascular inflammation in COVID-19 links focal microglial dysfunction with neuropathologies through IL-1/IL-6-related systemic inflammatory states
Authors: Fekete Rebeka , Simats Alba , Bíró Eduárd , Cserép Csaba , Schwarcz Anett D , Pósfai Balázs , Szabadits Eszter , Környei Zsuzsanna , Tóth Krisztina , Kellermayer Anna , Dávid Csaba , Acsády László , Kontra Levente , Silvestre-Roig Carlos , Moldvay Judit , Fillinger János , Hortobágyi Tibor , Liesz Arthur , Benkő Szilvia , Dénes Ádám 
Published: June 2023

AbstractCOVID-19 is associated with diverse neurological abnormalities, which predict poor outcome in patients. However, the mechanisms whereby infection-induced inflammation could affect complex neuropathologies in COVID-19 are unclear. We hypothesized that microglia, the resident immune cells of brain, are centrally involved in this process. To study this, we developed an autopsy platform allowing the integration of molecular anatomy-, protein- and mRNA data sets in post-mortem mirror blocks of brain and peripheral organ samples from COVID-19 cases. Nanoscale microscopy, single-cell RNA sequencing and analysis of inflammatory and metabolic signatures revealed distinct mechanisms of microglial dysfunction associated with cerebral SARS-CoV-2 infection. We observed focal loss of microglial P2Y12R at sites of virus-associated vascular inflammation together with dysregulated microglia-vascular-astrocyte interactions, Cx3Cr1-fractalkine axis deficits and mitochondrial failure in severely affected medullary autonomic nuclei and other brain areas. Microglial dysfunction occurs at sites of excessive synapse- and myelin phagocytosis and loss of glutamatergic terminals. While central and systemic viral load is strongly linked in individual patients, the regionally heterogenous microglial reactivity in the brain correlated with the extent of central and systemic inflammation related to IL-1 / IL-6 via virus-sensing pattern recognition receptors (PRRs) and inflammasome activation pathways. Thus, SARS-CoV-2-induced central and systemic inflammation might lead to a primarily glio-vascular failure in the brain, which could be a common contributor to diverse COVID-19-related neuropathologies.
Overexpression of the receptor-like kinaseBIR1gene causesSOBIR1- andEDS1-dependent cell death phenotypes in Arabidopsis
Authors: Guzmán-Benito Irene , Robinson Carmen , Punzón Isabel , Llave César 
Published: June 2023

AbstractThe receptor-like kinaseBAK1-INTERACTING RECEPTOR-LIKE KINASE 1(BIR1) functions as a negative regulator of multiple resistance signaling pathways inArabidopsis thaliana. Previous studies showed that loss ofBIR1function leads to extensive cell death and activation of constitutive immune responses. Here, we use a dexamethasone (DEX)-inducible expression system to investigate the impact ofBIR1overexpression on plant growth and development and immune regulation. We show that, in the absence of microbes or microbe-derived elicitors/effectors, plants that overexpressBIR1displayed cell death phenotypes that concur with the transcriptomic up-regulation of multiple immune pathways involved in pathogen perception and defense signal transduction.BIR1overexpression has similar loss-of-function effects toBIR1depletion in knockout plants, which suggests that properBIR1homeostasis requires a tight regulation ofBIR1expression within a functional threshold. We show thatENHANCED DISEASE SUSCEPTIBILITY 1(EDS1) andSUPPRESSOR OF BIR1-1(SOBIR1) are required for the effector-triggered immunity (ETI)-type cell death phenotypes associated with overexpression ofBIR1. Our data is then consistent with the current hypothesis by which loss ofBIR1regulation and/or integrity is sensed by one or several guarding resistance (NLR) proteins to initiate a cell death response, in which SOBIR1 cooperates with EDS1 to transduce signals downstream of R proteins.Summary StatementRegulation of the receptor-like kinaseBIR1has a strong impact on plant growth and development and immune homeostasis in Arabidopsis.BIR1overexpression causes cell death-and senescence-like phenotypes that requireEDS1andSOBIR1signaling pathways, and that resemble to those observed byBIR1depletion.
Type IV-A3 CRISPR-Cas systems drive inter-plasmid conflicts by acquiring spacersin trans
Authors: Benz Fabienne , Camara-Wilpert Sarah , Russel Jakob , Wandera Katharina G. , Čepaitė Rimvydė , Ares-Arroyo Manuel , Gomes-Filho José Vicente , Englert Frank , Kuehn Johannes , Gloor Silvana , Cuénod Aline , Aguilà-Sans Mònica , Maccario Lorrie , Egli Adrian , Randau Lennart , Pausch Patrick , Rocha Eduardo , Beisel Chase L. , Madsen Jonas S. , Bikard David , Hall Alex R. , Sørensen Søren J , Pinilla-Redondo Rafael 
Published: June 2023

ABSTRACTType IV-A CRISPR-Cas systems are primarily encoded on plasmids and form multi-subunit ribonucleoprotein complexes with unknown biological functions. In contrast to other CRISPR-Cas types, they lack the archetypical CRISPR acquisition module and encode a DinG helicase instead of a nuclease component. Type IV-A3 systems are carried by large conjugative plasmids that often harbor multiple antibiotic-resistance genes. Although their CRISPR array contents suggest a role in inter-plasmid conflicts, this function and the underlying mechanisms have remained unexplored. Here, we demonstrate that a plasmid-encoded type IV-A3 CRISPR-Cas system co-opts the type I-E adaptation machinery from its clinicalKlebsiella pneumoniaehost to update its CRISPR array. Furthermore, we demonstrate that robust interference of conjugative plasmids and phages is elicited through CRISPR RNA-dependent transcriptional repression. By targeting plasmid core functions, type IV-A3 can prevent the uptake of incoming plasmids, limit their horizontal transfer, and destabilize co-residing plasmids, altogether supporting type IV-A3’s involvement in plasmid competition. Collectively, our findings shed light on the molecular mechanisms and ecological function of type IV-A3 systems and have broad implications for understanding and countering the spread of antibiotic resistance in clinically relevant strains.
The Role of Respiratory Complex IV in Lifespan Length and Quality
Authors: Castejon-Vega Beatriz , Fernandez-Guerrero Ignacio , Myers Kevin , Kataura Tetsushi , Stefanatos Rhoda , Korolchuk Viktor I. , Sanz Alberto 
Published: June 2023

AbstractMitochondria play a pivotal role in lifespan regulation, though the underlying mechanisms remain elusive. As ageing progresses, damaged mitochondria with reduced ATP production and increased Reactive Oxygen Species (ROS) generation accumulate, yet mitochondrial depletion extends the lifespan of various animal models. Our previous research demonstrated that complex I (CI) activity during development but not adulthood is crucial for determining the lifespan ofDrosophila melanogaster. Still, CI-deficient mitochondria do not generate excessive ROS, failing to recapitulate mitochondrial ageing. In this study, we focus on complex IV (CIV), whose depletion leads to the accumulation of “old-mitochondria”, i.e. producing less ATP and more ROS. We reveal that CIV’s role in longevity is more intricate than CI’s, shaping lifespan through two “windows of opportunity”. The first window, shared by CI and CIV, occurs during development. Small perturbations in CIV during development lead to the emergence of short-lived flies. These flies exhibit an adult phenotype reminiscent of mitochondrial- associated diseases, primarily characterised by their inability to store fat efficiently. Accordingly, partial complementation of CIV function using an alternative oxidase (AOX) restores molecular and physiological phenotypes. The second window emerges during fly senescence, where CIV deficiency curtails lifespan without hastening ageing—flies die earlier but not more rapidly. Notably, only the developmental phenotype is associated with TOR dysregulation and altered autophagy, emphasising that developmental dysfunction uniquely interferes with nutrient sensing and the main cellular recycling pathway. This study sheds light on the multifaceted role of mitochondrial complex IV in modulating lifespan, providing potential targets for interventions to foster healthy ageing.
A conformationally constrained synthetic peptide efficiently inhibits malaria parasite entry into human red blood cells
Authors: Biswas Anamika , Narayan Akash , Sinha Suman , Mandal Kalyaneswar 
Published: June 2023

AbstractRestricting the conformational freedom of a peptide by backbone cyclization and incorporation of an additional disulfide bond leads to a unique cyclic peptide that inhibits the invasion of red blood cells by malaria parasites efficiently. The engineered peptide exhibits twenty fold enhanced affinity towards its receptor (PfAMA1) compared to the native peptide ligand (PfRON2).
Are we ready to transition to sustainable sheep production in New Zealand? A critical analysis from the perspectives of transition intention and sustainable tool adoption
Authors: Yang Wei , Phong Luu Quoc , De Silva Tracy-Anne , Penelope Jemma 
Published: June 2023

Purpose This study aims to understand New Zealand sheep farmers’ readiness toward sustainability transition by assessing their intentions of transition and adoption of sustainability tools, with information collection considered to mediate the intention–adoption relationship.Design/methodology/approach Based on the data collected from a survey of New Zealand sheep farmers in 2021, the empirical analysis was developed to investigate farmers’ perceptions of and attitudes toward readiness to move toward a sustainability transition. Structural equation modeling associated with principal component analysis was used to empirically test the theory of planned behavior constructs.Findings The results show that pressure from the public and the sheep industry, and the perceived controls of transition drive the intention of sustainability transition; farmers with higher intention of sustainability transition are found to be more likely to adopt sustainability tools. However, there is an attitude–behavior gap, wherein positive attitudes toward sustainability transition may not lead to a higher likelihood of adopting sustainability tools. There is no evidence of the mediating role of information collection on the intention–adoption relationship, while a positive effect was found in information collection on the adoption of sustainability tools.Practical implications The empirical evidence indicates that policymakers need to help increase the awareness of sustainable production and help farmers overcome barriers to achieving sustainable production by finding ways to turn intentions into adoption.Originality/value Being the first attempt to empirically assess farmers’ readiness toward sustainability transition, the study fills the gap of limited understanding of the link between sustainability transition intention and sustainable tools adoption in sustainability transition.
Breaking the glass ceiling for a sustainable future: the power of women on corporate boards in reducing ESG controversies
Authors: Issa Ayman , Hanaysha Jalal Rajeh 
Published: June 2023

Purpose
This study aims to investigate the relationship between board gender diversity and environmental, social and governance (ESG) controversies and to determine if a critical mass of female directors has a significant impact on ESG performance.

Design/methodology/approach
The study analyzes a sample of non-financial companies from 13 European countries between 2004 and 2021. The primary method used to reach conclusions was the pooled ordinary least squares regression. Additionally, the study used supplementary techniques such as alternative measurement, sub-sample analysis and two-stage least squares to enhance its reliability.

Findings
The results indicate that a higher representation of women on boards is correlated with a reduction in the number of ESG controversies, particularly when there are three or more female directors. Furthermore, the relationship between board gender diversity and ESG controversies may be affected by factors such as industry, governance and a company’s environmental performance.

Practical implications
This study suggests that increasing women’s representation on boards may mitigate ESG controversies and improve firm reputation and performance, especially in industries with high ESG risks. Policymakers can support this through policies, targets, training and inclusive practices. The findings also inform investors and stakeholders of the relationship between board gender diversity and ESG controversies.

Originality/value
This study expands the understanding of the relationship between board gender diversity and sustainable accounting and finance. It focuses on the effect that having female board members has on corporate policies, which is significant for shaping global policies that promote diversity on boards.
Impact of ethnic and cultural diversity on millennial living preferences and homeownership
Authors: Wu Yi , Tidwell Alan , Sah Vivek 
Published: June 2023

Purpose
This study aims to examine living preference and tenure among millennials, with a particular focus on the impact of ethnic and cultural diversity on housing outcomes including observed homeownership inequalities.

Design/methodology/approach
Using the individual panel data from three waves in American Housing Survey, 2015–2019, this study compares the likelihood of co-residing among Asian and Hispanic millennials with non-Hispanic white millennial peers. Furthermore, this study estimates the effect of co-residence on homeownership across generational and ethnic backgrounds.

Findings
This study finds a preference for coresident adult familial households among foreign-born Asian and Hispanic millennials, and US-born Hispanic millennials when compared to their non-Hispanic white millennial peers. The results are robust after considering neighborhood selection bias, affordability and education. The effect of co-residence on ownership is significant and positive, suggesting this living arrangement contributes to homeownership across all generational and ethnic groups.

Practical implications
Housebuilders should be aware of Asian and Hispanic millennials’ increased appetite for extended family living arrangements and consider increasing the physical size of affordable or workforce-oriented rental housing and new single family construction to accommodate more adult co-living arrangements.

Originality/value
This study provides a more comprehensive understanding of the role ethnic and cultural diversity has on millennial adult living preferences and its generational differences, which is not just “boomeranging” as identified by previous literature, contributing to the growing interest in the housing research on the effect of ethnic diversity and culture on millennials’ homeownership rates.
Increasing stakeholder engagement in research projects through standardization activities
Authors: Lindner René , Hernantes Josune , Jaca Carmen 
Published: June 2023

PurposeThis research assesses the implications of integrating standardization activities into European research projects to foster the engagement of project internal and external stakeholders and into different project stakeholder management theories.Design/methodology/approachThis paper analyzes the integration of standardization and the engagement of project internal and external stakeholders in standardization activities in a multi-case study of four European Framework Program projects and with the projects Advancing Resilience of Historic Areas Against Climate-Related and Other Hazards (ARCH) and Smart Mature Resilience (SMR) in two separate case studies more deeply. The multi-case study mainly evaluates the stakeholder participation in 10 CEN Workshop Agreements. While in the two case studies, among other things, two project surveys are used to investigate how stakeholder engagement was supported by standardization activities.FindingsThe results show that standardization significantly supports stakeholder engagement and lead to a proposal on how standardization can support achieving stakeholder engagement goals in the different research project phases.Originality/valueThis research provides practical information for policy-makers who support standardization as a tool for research, as well as for researchers and project managers who want to use standardization activities efficiently in research projects.
More burden less access: a study of sanitation issues in urban slums of Aligarh district
Authors: Iqrar Sanoobia , Musavi Azra 
Published: June 2023

PurposeThe present study intends to highlight the issues and challenges pertinent to sanitation practices among urban slum women in India.Design/methodology/approachThe primary qualitative data has been collected from 100 households sampled by stratified random and purposive sampling through in-depth interviews, and a structured interview schedule and themes were developed accordingly.FindingsThe main theme-based results of the study included the challenges of open defecation in slums, public toilets as an alternative, sanitation-related stress in slum women, compromised state of health of women and children, menstrual hygiene among women in slums and increasing burden on women in terms of water management. The study recommends the creation of toilets in every slum locality with maintenance and awareness to be raised.Originality/valueUrban sanitation has received less research than rural sanitation. The present study focused on the issues of urban sanitation, which are often neglected. To ensure the study's uniqueness, researchers visited and mentioned each open defecation site to understand the situation.Peer reviewThe peer-review history for this article is available at: https://publons.com/publon/10.1108/IJSE-12-2022-0782
Simulation experiment study on friction and wear of materials under high humidity conditions
Authors: Zhang Meixian , Ou Yiding , Yuan Haibing , Pan Junlin , Ma Lei 
Published: June 2023

Purpose
The purpose of this paper is to examine the practicability of the self-designed ambient humidity controllable pin-disc/rolling multifunctional friction and wear test device and to evaluate the friction and wear characteristics of materials under diverse ambient humidity conditions in different contact forms.

Design/methodology/approach
The practicability of the self-designed multifunctional friction tester was examined by the friction and wear tests of materials under different ambient humidity conditions [65%RH, 98%RH (relative humidity)] in diverse contact forms (pin/disc and rolling). Meanwhile, the friction and wear properties of pin/disc samples also rolling samples were assessed from three aspects: average friction coefficient, wear mass and wear morphology.

Findings
The results prove that the self-designed multifunctional friction tester has practicability. Therefore, it can be used to simulate the friction and wear tests of materials under diverse ambient humidity conditions in different contact forms. Besides, it is evident that the wear damage of pin/disc and rolling samples are greatly improved under high ambient humidity conditions. And when other conditions are identical, the higher the ambient humidity, the smaller the average friction coefficient, wear mass and wear damage degree of pin/disc also rolling samples.

Originality/value
This paper offers a self-designed multifunctional friction and wear test device. And the tester not only can realize the control of test ambient humidity, but also achieve the wear test of pin/disc or rolling contact forms. The design and production of the tester can offer convenience for the research of tribology, and provide fundamental guidance for the study of materials under high humidity condition in diverse contact forms.
HyFlex pedagogy: six strategies supported by design-based research
Authors: Howell Emily , Hubbard Koti , Linder Sandra , Madison Stephanie , Ryan Joseph , Bridges William C. 
Published: June 2023

PurposeThis study investigates the following research question: What pedagogical strategies are necessary for the success of HyFlex course design? The findings to this question are based in new media literacies and help to further pedagogy in an emerging HyFlex model while also grounding in needed theorization.Design/methodology/approachThis study uses design-based research (DBR) across two iterations and four doctoral, higher education courses, using mixed methods of data collection and analysis.FindingsSix pedagogical strategies influential for HyFlex research are presented, each grounded in a new media literacy skill.Originality/valueThese six pedagogical strategies help practitioners grappling with the HyFlex or blended learning model merge traditional pedagogy with how this might be tailored for students entrenched in a participatory culture.
An empirical assessment of the effects of background, institutional support and peer effects on Black college student success
Authors: Mao En , Meder Martin E. , Zhang Jing 
Published: June 2023

PurposeThis research explores the key factors that contribute to the success of Black students in a predominantly White institution (PWI). Two measures of success are examined: cumulative grade point average (GPA) and graduation status.Design/methodology/approachUsing student-level data from a southeastern university, this research estimates education production functions using ordinary least squares regression.FindingsWhile the negative effect of being Black is significant for both cumulative GPA and graduation status, the effect becomes overshadowed when peer effects are added. The authors also found the critical effect of institutional support on student success.Research limitations/implicationsThe student-level data are restricted to a single institution over a relatively short period of time, which limits the authors' ability to analyze institution-level factors.Practical implicationsThis research provides a broad view of many significant factors for student success with particular highlights on the importance of encouraging Black students to utilize institutional support.Originality/valueThis study is an extension of the education production function model in the field of student success. The study identified peer effects and institutional support as more powerful determinants of student success than race.