bluuebunny/crossref_metadata_2025_split huggingface dataset:
PENGEMBANGAN MEDIA PEMBELAJARAN MENGGUNAKAN SOFTWARE ADOBE FLASH PROFESIONAL CS8 PADA MATERI SEGI EMPAT
Authors: Ashari Ashar Jurang Penatas 
Published: March 2023

Learning media is important in the learning process, however the use of learning media that is not appropriate to the needs of students will complicate the learning process. At SMPN 1 Mojowarno students are only asked to read textbook then they are given assigments via the whatsapp application. In addition, the rectangular material at the school only explained the formulas without ane explanation of how the formulas could be found, thus make the learning process less meaningfull The aims of this developmen is to produce learning media in the form of Adobe Flash Professioanal CS8 software that is valid, practical, and effective on rectangle material in online learning. The type of research used in this study was Research and Development by adopting the ADDIE development model. The stages carried out in this research are, Analysis, Design, Development, Implementation, and Evaluation. The result of material expert validation showed a percentage of 86,6% with very valid criteria, and the result of media expert validation showed a percentage of 88% with very valid citeria. The result of students response questionnaire showed a percentage of 84,7% in the practical category, and the individual learning outcomes test showed a average 80,9 and percentage of classical test 82,35% with effective category. So it can be concluded that the learningmedia that has been developed using Adobe Flash Professional software on rectangular material in online learning has met valid, practical and effective criteria.
Keywords: Adobe Flash Professional CS8, Rectangel, Online learning
ANALISIS KEMAMPUAN PEMODELAN MATEMATIS ANAK-ANAK DI KAMPUNG ADAT BOTI
Authors: Tanu Melkianus , Ralmugiz Uke , Yusuf St. Muthmainnah 
Published: March 2023

Analysis of the Children’s Mathematical Modeling Ability in Boti Traditional Village, Kupang. Academic Year 2020/2021. Skripsi. Mathematics Education Study Program. Faculty of Teacher Training and Education. University of Muhammadiyah Kupang.
This study aims to determine the mathematical modeling ability of children in the traditional village of Boti, which is very necessary in solving contextual problems completely because they do not understand the problem and do not able to convert the problem into a mathematical model. This research isqualitative research with a descriptive approach. The subjects of this study were 7 children in Boti traditional village. There were 3 interview test respondents who were selected based on the high, medium, and low ability categories. Data collection techniques used were data reduction, data presentation, and drawing conclusion. The validity of the data tested using a triangulation of data technique. Based on the results of 3 essay test items, it showed that the mathematical modeling ability has 3 categories, namely: 1 high-ability child, 4 moderately capable children, and 2 low-ability children.
An Assessment of Crop-Specific Land Cover Predictions Using High-Order Markov Chains and Deep Neural Networks
Authors: Sartore Luca , Boryan Claire , Dau Andrew , Willis Patrick 
Published: March 2023

High-Order Markov Chains (HOMC) are conventional models, based on transition probabilities, that are used by the United States Department of Agriculture (USDA) National Agricultural Statistics Service (NASS) to study crop-rotation patterns over time. However, HOMCs routinely suffer from sparsity and identifiability issues because the categorical data are represented as indicator (or dummy) variables. In fact, the dimension of the parametric space increases exponentially with the order of HOMCs required for analysis. While parsimonious representations reduce the number of parameters, as has been shown in the literature, they often result in less accurate predictions. Most parsimonious models are trained on big data structures, which can be compressed and efficiently processed using alternative algorithms. Consequently, a thorough evaluation and comparison of the prediction results obtain using a new HOMC algorithm and different types of Deep Neural Networks (DNN) across a range of agricultural conditions is warranted to determine which model is most appropriate for operational crop specific land cover prediction of United States (US) agriculture. In this paper, six neural network models are applied to crop rotation data between 2011 and 2021 from six agriculturally intensive counties, which reflect the range of major crops grown and a variety of crop rotation patterns in the Midwest and southern US. The six counties include: Renville, North Dakota; Perkins, Nebraska; Hale, Texas; Livingston, Illinois; McLean, Illinois; and Shelby, Ohio. Results show the DNN models achieve higher overall prediction accuracy for all counties in 2021. The proposed DNN models allow for the ingestion of long time series data, and robustly achieve higher accuracy values than a new HOMC algorithm considered for predicting crop specific land cover in the US.
Mycoremediation of Heavy Metals from Electronic Waste Polluted Water Using Indigenous Fungi and Its Implications
Authors: Majolagbe O. N. , Ariyo O. G. , Bello O. S. , Adeyeni E. G. , Owoseni A. A. , Oluranti O. O. 
Published: March 2023

Background: Heavy metals (HM) pollutants are crucial environmental and public health problems due to their toxicity, which has implications on public health. The site of study has been reported to be densely polluted with heavy metals as a result of dumping used electronic wastes into the water body.
Aim: This study was carried out to determine the biosorption potentials of indigenous fungi isolates in reducing heavy metal present in electronic wastes polluted water body.
Study Design: This was a laboratory based study.
Place and Duration of Study: The study was conducted at the Microbial Resources Research Laboratory, Department of Pure and Applied Biology, Ladoke Akintola University of Technology Ogbomoso, Nigeria from July 2021 to June 2022.
Methodology: Heavy metal polluted water sample was collected near the dumpsite of e-waste in Alaba International market, Lagos Nigeria. Fungi were isolated from the polluted water sample by carrying out serial dilution. Pure colonies were obtained and stored at 4°C. Media formulations (MF) trials for the biosorption process was achieved using brewery waste and honeycomb extracts. Exactly 200 ml of MF were dispensed into a 500 ml Elmeryer flask containing 100 ml of the e-waste polluted water and fugal discs. Atomic absorption spectrophotometer was used to determine the heavy metal concentration in the water samples. The biological interactions of the fungi with the polluted water sample was monitored using Fourier transform infrared (FTIR) spectroscopy. The pH, electrical conductivity and other physicochemical parameters were also determined.
Results: Trichoderma harzianum, T. viride, Fusarium oxysporum, Aspergillus flavus and A. niger were isolated from the heavy metal polluted water samples. Heavy metal such as Pb (13.30 mg/L), Cd (16.50 mg/L), Cr (6.41 mg/L), Ni (3.81 mg/L), Zn (8.85 mg/L), Cu (8.33 mg/L), Fe (5.60 mg/L) had values which were higher than the acceptable limits. Biosorption efficiency of each of the fungus in reducing the metals present in the sample was in the increasing order of A. niger<A. flavus<F. oxysporum<T. viride<T. harzianum. FTIR showed that some peaks were shifted to lower wavenumber as a result of the interactions of the fungus with the heavy metal in the water sample. This study revealed that T. harzianum had the highest biosorption efficiency for the removal of heavy metal in the polluted water sample.
Conclusion: It is essential that heavy metals be removed from polluted water body to avoid its deleterious effects on public health. This can be achieved biologically using any of the fungi isolated in this work.
Quantitative determination of SLC2A1 variant functional effects in GLUT1 deficiency syndrome
Authors: Tayebi Naeimeh , Leon‐Ricardo Brian , McCall Kevin , Mehinovic Elvisa , Engelstad Kristin , Huynh Vincent , Turner Tychele N. , Weisenberg Judy , Thio Liu L. , Hruz Paul , Williams Robin S. B. , De Vivo Darryl C. , Petit Vincent , Haller Gabe , Gurnett Christina A. 
Published: March 2023

AbstractObjectiveThe goal of this study is to demonstrate the utility of a growth assay to quantify the functional impact of single nucleotide variants (SNVs) in SLC2A1, the gene responsible for Glut1DS.MethodsThe functional impact of 40 SNVs in SLC2A1 was quantitatively determined in HAP1 cells in which SLC2A1 is required for growth. Donor libraries were introduced into the endogenous SLC2A1 gene in HAP1‐Lig4KO cells using CRISPR/Cas9. Cell populations were harvested and sequenced to quantify the effect of variants on growth and generate a functional score. Quantitative functional scores were compared to 3‐OMG uptake, SLC2A1 cell surface expression, CADD score, and clinical data, including CSF/blood glucose ratio.ResultsNonsense variants (N = 3) were reduced in cell culture over time resulting in negative scores (mean score: −1.15 ± 0.17), whereas synonymous variants (N = 10) were not depleted (mean score: 0.25 ± 0.12) (P < 2e‐16). Missense variants (N = 27) yielded a range of functional scores including slightly negative scores, supporting a partial function and intermediate phenotype. Several variants with normal results on either cell surface expression (p.N34S and p.W65R) or 3‐OMG uptake (p.W65R) had negative functional scores. There is a moderate but significant correlation between our functional scores and CADD scores.InterpretationCell growth is useful to quantitatively determine the functional effects of SLC2A1 variants. Nonsense variants were reliably distinguished from benign variants in this in vitro functional assay. For facilitating early diagnosis and therapeutic intervention, future work is needed to determine the functional effect of every possible variant in SLC2A1.
Crocetin Regulates Functions of Neural Stem Cells to Generate New Neurons for Cerebral Ischemia Recovery
Authors: Liu Zhongqing , Wang Zhaojun , Zhu Zhanchi , Hong Jing , Cui Leisha , Hao Ying , Cheng Guosheng , Tan Rui 
Published: March 2023

AbstractMany neurons undergo apoptosis after ischemic stroke. In the brain, neurogenesis has the potential for neuronal replacement and can be activated by external conditions to repair the injury. Crocetin (CRO), naturally extracted from the plant saffron, acts as a neuroprotective agent for ischemic stroke. However, the underlying mechanism remains unknown. In this work, the effect of CRO on neural stem cell (NSC) behaviors and subventricular zone neurogenesis is investigated. Initially, NSCs are incubated with different concentrations of CRO to detect the cell proliferation and differentiation in vitro. Second, ischemic stroke induced rats are treated with CRO using nimodipine (NMDP) as a comparison. The behavioral functions, infarcted volume, and apoptotic Nissl bodies of rats are noticeably improved after CRO‐treatment, comparable to those of NMDP. In addition, the increased regional cerebral blood flow and promoted neuronal differentiation are achieved by CRO‐treatment. Brain tissue examination shows significantly increased neuronal regeneration in the focal ischemic injury area. Meanwhile, the length of neurites is prolonged, indicating that CRO could potentially promote neurite extension to enhance cell–cell communication. These findings demonstrate that CRO facilitated the neuronal differentiation of NSCs by activating subventricular zone neurogenesis in damaged cortex and striatum sites to repair ischemic stroke.
The case for remote supervision
Authors: Boulanger Ghislaine , Rosenberg Larry 
Published: March 2023

AbstractAs the necessity of meeting state regulations has continued to mount in the public mental health sector, and insurance companies limit the number of therapy sessions covered in a year, the emphasis on evidence‐based treatments, most often in the guise of some form of Cognitive Behavioral Therapy, continues unabated. Meanwhile, knowledge about and the ability to practice psychodynamically have all but vanished from most community mental health clinics in the United States. With shrinking funds, increasing demand for services, and increasing costs, therapists and their supervisors in these settings are expected to meet productivity and documentation requirements imposed by state and federal regulations and by the clinics themselves. These demands leave little time for therapists to discuss actual cases with their supervisors, to reflect on their experiences with patients and to wonder about the patient's experience with them, skills that are in themselves among the essential tools of psychodynamic clinical training and are crucial to patient care. This paper summarizes the qualitative data collected at the conclusion of a pilot study in which a dozen staff therapists in a community mental health center met weekly individually with psychodynamic supervisor/consultant volunteers to discuss their ongoing cases.
ITGA5 promotes tumor angiogenesis in cervical cancer
Authors: Xu Xiaohan , Shen Lulu , Li Wenhan , Liu Xiaoli , Yang Ping , Cai Jing 
Published: March 2023

AbstractPurposeIntegrins are critical to cancer progression. Integrin alpha 5 (ITGA5) is correlated with the prognosis of cervical cancer patients. However, whether ITGA5 plays an active role in cervical cancer progression or not remains unknown.MethodsITGA5 protein expression was detected in 155 human cervical cancer tissues by immunohistochemistry. Data from The Cancer Genome Atlas were utilized to identify risk factors for the overall survival of cervical cancer patients and ITGA5‐associated differentially expressed genes. Analyses of single‐cell RNA‐seq based on Gene Expression Omnibus datasets were performed to show the coexpression of ITGA5 and angiogenesis factors. Tube formation assay, 3D spheroid sprout assay, qRT‐PCR, Western Blotting, ELISA, and immunofluorescence were conducted to explore the angiogenic function of ITGA5 in vitro and underlying mechanisms.ResultsHigh ITGA5 level was significantly correlated with increased risk in terms of overall survival and advanced disease stage in cervical cancer patients. ITGA5‐associated differentially expressed genes linked ITGA5 to angiogenesis, and immunohistochemistry showed a positive correlation between ITGA5 and microvascular density in cervical cancer tissues. Moreover, tumor cells transfected with ITGA5‐targeting siRNA decreased ability to promote endothelial tube formation in vitro. ITGA5/VEGFA coexpression was observed in a tumor cell subpopulation and the decreased endothelial angiogenesis by downregulating ITGA5 could be reversed by VEGFA. Bioinformatics analysis highlighted the PI3K‐Akt signaling pathway as downstream of ITGA5. Downregulation of ITGA5 in tumor cells significantly decreased p‐AKT and VEGFA levels. Fibronectin (FN1) coated cells or transfected with FN1‐targeting siRNA showed fibronectin may play a critical role on ITGA5‐mediated angiogenesis.ConclusionITGA5 promotes angiogenesis and possibly be a potential predictive biomarker for poor survival of patients in cervical cancer.
A semiautomatic segmentation method framework for pelvic bone tumors based on CT‐MR multimodal images
Authors: Ge Qi , Xia Tienan , Qiu Yan , Liu Jinxin , Shang Guanning , Liu Bin 
Published: March 2023

AbstractThe pelvic structure is complex and the tumor is poorly defined from the surrounding tissues. Finding the exact tumor resection margin based on the surgeon's clinical experience alone is a time‐consuming and difficult task, which is a major factor leading to surgical failure. An accurate method for segmenting pelvic bone tumors is needed. In this paper, a semiautomatic segmentation method for pelvic bone tumors based on CT‐MR multimodal images is presented. The method combines multiple medical prior knowledge and image segmentation algorithms. Finally, the segmentation results are visualized in three dimensions. We tested the proposed method on a collection of 10 cases (97 tumor MR images in total). The segmentation results were compared with the manual annotation of the physicians. On average, our method has an accuracy of 0.9358, a recall of 0.9278, an IOU value of 0.8697, a Dice value of 0.9280, and an AUC value of 0.9632. The average error of the 3D model was within the allowable range of the surgery. The proposed algorithm can accurately segment bone tumors in pelvic MR images regardless of tumor location, size, and other factors. It provides the possibility to assist pelvic bone tumor preservation surgery.
Fine needle aspiration cytology of follicular dendritic cell sarcoma of the stomach masquerading as gastrointestinal stromal tumor: A case report of a unique entity with emphasis on cytomorphology
Authors: Shacklette Alex H. , Chen Shaoxiong , DeWitt John M. , Patel Krunal S. , Saeed Omer A. 
Published: March 2023

AbstractFollicular dendritic cell sarcoma (FDCS) is a rare malignant neoplasm, postulated to arise from follicular dendritic cells, with approximately 343 reported cases. Less than 100 cases of FDCS were in the gastrointestinal tract, with only four cases described in the stomach, none of them diagnosed on fine needle aspiration (FNA) cytology. We report here the first case of FDCS of the stomach diagnosed on FNA. Our patient is a 31‐year‐old male who presented with several years history of intermittent abdominal pain prompting occasional emergency‐room visits. Imaging showed a 10.6 cm mass arising from the stomach, concerning for gastrointestinal stromal tumor. FNA cytology was performed using five passes with a 22‐gauge needle. The smears were moderately cellular consisting of sheets and large, loosely cohesive clusters of ovoid to spindle cells with indistinct cytoplasmic borders and abundant cytoplasm, peppered with numerous small mature lymphocytes. The nuclei of the tumor cells were oval with finely granular chromatin with frequent nuclear grooves, pseudoinclusions, and easily recognizable mitotic figures. The tumor cells were positive for FDCS markers (CD21, CD23, and CD35).
Characterizing expression pattern of Six2Cre during mouse craniofacial development
Authors: Umar Meenakshi , Dong Chunmin , He Fenglei 
Published: March 2023

SummaryCraniofacial development is a complex process involving diverse cell populations. Various transgenic Cre lines have been developed to facilitate studying gene function in specific tissues. In this study, we have characterized the expression pattern of Six2Cre mice at multiple stages during craniofacial development. Our data revealed that Six2Cre lineage cells are predominantly present in frontal bone, mandible, and secondary palate. Using immunostaining method, we found that Six2Cre triggered reporter is co‐expressed with Runx2. In summary, our data showed Six2Cre can be used to study gene function during palate development and osteogenesis in mouse models.
The Ontario Line, another milestone in developing Toronto's public transit system
Authors: Neugebauer Erich , Chesser David , Meyer Veronica , Fromberger Thomas 
Published: March 2023

AbstractThe new Ontario Line is one of four priority transit projects announced by the province of Ontario in 2019 for the Greater Toronto Area (GTA). It will be a 15.6 km stand‐alone rapid transit line that will run through the city from Exhibition/Ontario Place in the southwest, through the heart of downtown Toronto, all the way to the Ontario Science Centre in the northeast. Over half of the route (9 km) is planned to run underground through new tunnels, with the remainder running along elevated and at‐grade rail corridor sections of track. Fifteen new stations are proposed, whereby eight are planned as deep underground structures. Most of these underground stations will be built in the downtown core, with numerous connections to the broader transit network of the GTA, including GO Transit rail services, the Toronto Transit Commission's (TTC) existing subway Lines 1 and 2, the Line 5 (Eglinton Crosstown LRT) currently under construction, as well as numerous bus and streetcar routes. The expected project benefits of Ontario Line are as follows:

A faster and more reliable access to rapid transit system with more than 227,500 people living within walking distance to the new line.
Reduction in crowding of existing Line 1 subway.
Up to 47,000 jobs accessible by transit in 45 min or less for Toronto residents.
Economic and community growth along the future transit line and significant reduction in traffic congestion and greenhouse gases by providing alternative transportation options.

The Ontario Line is currently being delivered through a number of contracts with different procurement approaches.
Circ_0073228 serves as a competitive endogenous ribonucleic acid to facilitate proliferation and inhibit apoptosis of hepatocellular carcinoma cells by the miR‐139‐5p/deoxyribonuclease II axis
Authors: Bai Lu , Yu Zujiang , Li Qinggang , Hu Xiaobo , Liu Xiaorui , Li Fulei , Li Shasha , Chen Yanling 
Published: March 2023

AbstractBackgroundHepatocellular carcinoma (HCC) is a common malignancy and has extremely poor prognosis and outcome. Homo sapiens deoxyribonuclease II (DNASE2) has been reported to participate in HCC progression. Here, the role of DNASE2 in HCC cells and the putative upstream circRNA that mediates DNASE2 expression was investigated.MethodsThe expression of RNAs in liver hepatocellular carcinoma (LIHC) samples was analyzed by bioinformatic analysis. The proliferation, apoptosis, migration, invasion, and gene expression in HCC cells were investigated using a Cell Counting Kit ‐8, colony formation, flow cytometry analysis, wound healing, transwell, western blotting, and a quantitative reverse transcriptase‐PCR. The binding relationship among circ_0073228, miR‐139‐5p and DNASE2 was measured by RNA pulldown and luciferase reporter assays.ResultsDNASE2 knockdown inhibited proliferation and promoted apoptosis of HCC cells, whereas DNASE2 overexpression showed the opposite results. miR‐139‐5p targeted DNASE2 and suppressed its expression. Overexpression of miR‐139‐5p inhibited malignant phenotypes of HCC cells. RPS23‐derived circ_0073228, which bound to miR‐139‐5p, was found to be upregulated in HCC cells. Inhibition of miR‐139‐5p or overexpression of DNASE2 counteracted the inhibitory effects of circ_0073228 knockdown on HCC cell progression.Conclusionscirc_0073228 serves as an oncogene to facilitate growth and inhibit apoptosis of HCC cells by regulating the miR‐139‐5p/DNASE2 axis.
Conversations of life and death: Experiences of death awareness and Death Café participation
Authors: Seifu Feven , Morgan Michael M. , DeDiego Amanda C. , Rassier Ayn 
Published: March 2023

AbstractTo understand the experiences of Death Café attendees and facilitators, the current qualitative study entailed interviews with nine participants focused on mortality awareness. Discussion of findings explores how mortality awareness influences culture and values. Implications explore how death and mortality can be addressed in counseling practice.