bluuebunny/crossref_metadata_2025_split huggingface dataset:
Functional characterization and molecular fingerprinting of potential phosphate solubilizing bacterial candidates from Shisham rhizosphere
Authors: Joshi Samiksha , Gangola Saurabh , Jaggi Vandana , Sahgal Manvika 
Published: April 2023

AbstractPhosphate solubilizing bacteria (PSB) are important role players in plant growth promotion. In the present study, we aimed to screen the functionally active phosphate solubilizing bacteria (PSB) associated with Dalbergia sissoo Roxb. (Shisham) from different provenances. Screening for phosphate solubilization was done on Pikovskaya's agar, and 18 bacteria positive for the tri-calcium phosphate (Ca3(PO4)2 solubilization showing visible dissolution halo zones were identified. All 18 isolates showed zinc solubilization, indole acetic acid (IAA), siderophore, and hydrogen cyanide (HCN) production. The morphological and biochemical characterization with 16S rDNA gene-based phylogenetic analysis identified bacterial strains as belonging to the genus Pseudomonas, Klebsiella, Streptomyces, Pantoea, Kitasatospora, Micrococcus, and Staphylococcus. Among all the isolates, one of the isolates named L4, from Lacchiwala region was the most efficient P solubilizer with a high P solubilization index (4.75 ± 0.06) and quantitative P solubilization activity (891.38 ± 18.55 μg mL−1). The validation of phosphate solubilization activity of PSB isolates was done by amplification of the Pyrroloquinoline quinone (PQQ) genes, pqqA and pqqC. Based on this study, we have selected the bacterial strains which are efficient phosphate solubilizers and could be economical and eco-friendly in plant growth promotion, disease suppression, as an antioxidant, and for subsequent enhancement of yield.
The nematicidal potential of novel fungus, Trichoderma asperellum FbMi6 against Meloidogyne incognita
Authors: Saharan Ritul , Patil J. A. , Yadav Saroj , Kumar Anil , Goyal Vinod 
Published: April 2023

AbstractOne of the most damaging pests in vegetable crops is the root-knot nematode (Meloidogyne incognita) worldwide. The continuous use of nematicide is costly and has unintended consequences for human and environmental health. To minimize nematicides, eco-friendly integrated nematode management is required. Trichoderma, an antagonistic fungus has been explored to control root-knot nematode. The fungal bio-control strain FbMi6 was identified as Trichoderma asperellum (accession no. MT529846.1). T. asperellum FbMi6 showed substantial nematicidal activity in the laboratory, with egg hatch suppression (96.6%) and juvenile mortality (90.3%) of M. incognita. T. asperellum FbMi6 was examined under pot and field conditions (after neem cake enrichment), both alone and in combination, and compared with controls. Application of T. asperellum FbMi6 enriched neem cake (1-ton ha-1) increased (28.3%) the okra yield and decreased (57.1%) nematode population as compared with control. T. asperellum FbMi6 enriched neem cake had higher polyphenol content (resistance enhancer) in okra compared with inoculated check.
Chopped straw and coffee husks affect bedding chemical composition and the performance and foot pad condition of broiler chickens
Authors: Biesek Jakub , Banaszak Mirosław , Grabowicz Małgorzata , Wlaźlak Sebastian 
Published: April 2023

AbstractBedding material is a crucial environmental factor for chickens. Coffee husks are waste from the industry that could be reused. The study aimed to analyze the chemical composition of various bedding types and assess their impact on the performance characteristics of broiler chickens and the incidence of footpad dermatitis (FPD). Ross 308 broilers were used in the study. Birds were divided into 3 groups (216 chickens, 72 per group). Group 1 was kept on chopped straw (S). Group 2 was kept on coffee husks (CHs), and the 3rd group (PB) was kept on pellet bedding made from S and CHs in a 1:1 ratio. The dry matter (DM), crude fiber (CF), nitrogen (N), phosphorus (P), potassium (K) content, and the pH of the bedding were analyzed. Production results were examined during 42 days of rearing. FPD was assessed on a point scale. The tissue composition of the carcasses and qualitative physicochemical characteristics of the meat (pH, color, water holding capacity, drip loss) and bone-breaking strength were analyzed. Straw had a higher CF content. In total, the highest N content in CHs was noticed. P content was lower in the S, and K was the highest in the CHs group, followed by S and PB. The pH of the bedding was lower in the CHs group, higher in PB, and highest in S bedding. The DM was decreasing within the days of rearing. A significant interaction was demonstrated between the type of bedding and rearing days on the bedding (manure) composition. On days 1–14, FCR deteriorated in the PB group compared to the S group. The presence of FPD was significantly lower in the PB group than in the others. In the PB group, chickens had a higher slaughter yield than in the S group and a lower weight and liver share than in the CHs group. The weight and proportion of abdominal fat were higher in the group kept on S than on CHs. It can be concluded that coffee husks as a component of pellets had a beneficial effect on reducing FPD in chickens and their slaughter yield and enriched bedding with nutrients, which with poultry manure, could be a good fertilizer for agricultural soils after rearing. It could be recommended to use pelleted bedding.
Minimal requirements for ISO15189 validation and accreditation of three next generation sequencing procedures for SARS-CoV-2 surveillance in clinical setting
Authors: Maschietto Céline , Otto Gaëtan , Rouzé Pauline , Debortoli Nicolas , Bihin Benoît , Nyinkeu Lesly , Denis Olivier , Huang Te-Din , Mullier François , Bogaerts Pierre , Degosserie Jonathan 
Published: April 2023

AbstractRapid and recurrent breakthroughs of new SARS-CoV-2 strains (variants) have prompted public health authorities worldwide to set up surveillance networks to monitor the circulation of variants of concern. The use of next-generation sequencing technologies has raised the need for quality control assessment as required in clinical laboratories. The present study is the first to propose a validation guide for SARS-CoV-2 typing using three different NGS methods fulfilling ISO15189 standards. These include the assessment of the risk, specificity, accuracy, reproducibility, and repeatability of the methods. Among the three methods used, two are amplicon-based involving reverse transcription polymerase chain reaction (Artic v3 and Midnight v1) on Oxford Nanopore Technologies while the third one is amplicon-based using reverse complement polymerase chain reaction (Nimagen) on Illumina technology. We found that all methods met the quality requirement (e.g., 100% concordant typing results for accuracy, reproducibility, and repeatability) for SARS-CoV-2 typing in clinical setting. Additionally, the typing results emerging from each of the three sequencing methods were compared using three widely known nomenclatures (WHO, Pangolineage, and Nextclade). They were also compared regarding single nucleotide variations. The outcomes showed that Artic v3 and Nimagen should be privileged for outbreak investigation as they provide higher quality results for samples that do not meet inclusion criteria for analysis in a clinical setting. This study is a first step towards validation of laboratory developed NGS tests in the context of the new European regulation for medical devices and in vitro diagnostics.
Male germ cell proliferation and apoptosis in sexually immature meagre Argyrosomus regius (Asso, 1801) treated with recombinant follicle stimulating hormone
Authors: Zupa Rosa , Duncan Neil , Giménez Ignacio , Mylonas Constantinos C. , Pousis Chrysovalentinos , Passantino Letizia , Cuko Rezart , Corriero Aldo 
Published: April 2023

AbstractThe meagre Argyrosomus regius (Asso, 1801) is a marine fish species that has an increasing aquaculture production in Europe. Lowering the age at maturity of hatchery-produced juveniles would support meagre aquaculture by reducing time between generations in selective breeding programs and reducing industrial costs for broodstock maintenance. The aim of this work was to assess the effects of a treatment with recombinant follicle stimulating hormone (rFsh), produced in ovarian cells of Chinese hamsters, on male germ cell proliferation and apoptosis in sexually immature meagre. The rFsh-treated fish had higher gonadosomatic index, larger seminiferous tubules, more abundant luminal spermatozoa, a lower density of anti-PCNA positive single A spermatogonia, a higher density of anti-PCNA positive spermatocysts and a lower incidence of germ cell apoptosis than control groups. The present study demonstrated the effectiveness of the produced rFsh in stimulating testis development and spermatogenesis in pre-pubertal meagre. Moreover, the rFsh treatment proved to be highly efficient in removing the apoptotic block of spermatogenesis observed in juvenile meagre, allowing spermatogonial survival and progress towards meiosis. The administration of rFsh did not stimulate spermatogonial self-renewal, a process whose control still needs to be elucidated.
On-admission and dynamic trend of laboratory profiles as prognostic biomarkers in COVID-19 inpatients
Authors: Siavoshi Fatemeh , Safavi-Naini Seyed Amir Ahmad , Shirzadeh Barough Siavash , Azizmohammad Looha Mehdi , Hatamabadi Hamidreza , Ommi Davood , Jalili Khoshnoud Reza , Fatemi Alireza , Pourhoseingholi Mohamad Amin 
Published: April 2023

AbstractThis large-scale study aimed to investigate the trend of laboratory tests of patients with COVID-19. Hospitalized confirmed and probable COVID-19 patients in three general hospitals were examined from March 20, 2020, to June 18, 2021. The confirmed and probable COVID-19 patients with known outcomes and valid laboratory results were included. The least absolute shrinkage and selection operator (LASSO) and Cox regression were used to select admittance prognostic features. Parallel Pairwise Comparison of mortality versus survival was used to examine the trend of markers. In the final cohort, 11,944 patients were enrolled, with an in-hospital mortality rate of 21.8%, mean age of 59.4 ± 18.0, and a male-to-female ratio of 1.3. Abnormal admittance level of white blood cells, neutrophils, lymphocytes, mean cellular volume, urea, creatinine, bilirubin, creatine kinase-myoglobin binding, lactate dehydrogenase (LDH), Troponin, c-reactive protein (CRP), potassium, and creatinine phosphokinase reduced the survival of COVID-19 inpatients. Moreover, the trend analysis showed lymphocytes, platelet, urea, CRP, alanine transaminase (ALT), and LDH have a dissimilar trend in non-survivors compared to survived patients. This study proposed a novel approach to find serial laboratory markers. Serial examination of platelet count, creatinine, CRP, LDH, and ALT can guide healthcare professionals in finding patients at risk of deterioration.
Peptide microarray-based identification of dormancy-associated Mycobacterium tuberculosis antigens inducing immune responses among latent tuberculosis infection individuals in Thailand
Authors: Hanthamrongwit Jariya , Aruvornlop Panicha , Saelee Chutiphon , Wanta Nattiya , Poneksawat Passarun , Soe Phyu Thwe , Kyaw Soe Paing , Khaenam Prasong , Warit Saradee , Valentini Davide , Mahasirimongkol Surakameth , Dhepakson Panadda , Soonthornchartrawat Sakulrat , Chootong Patchanee , Leepiyasakulchai Chaniya 
Published: April 2023

AbstractMulti-stage tuberculosis (TB) vaccines composed of active- and dormancy-associated antigens are promising to trigger the immune protection against all TB stages. However, scientists are still in quest of the suitable vaccine candidates. In this study, we identified the potential targets for this vaccine in a high TB burden country, Thailand. Peptide microarray was applied to gauge IgA and IgG antibodies specific to 16,730 linear epitopes of 52 dormancy-associated Mycobacterium tuberculosis (M. tb) proteins in three study groups: active tuberculosis (ATB), latent tuberculosis infection (LTBI) and endemic healthy control (EHC). Preferential IgA recognition against epitopes of dormancy-associated proteins was identified in LTBI group. Validation of these findings revealed that LTBI subjects exhibited the greater levels of Rv2659c- and Rv1738-specific IgA than those of household contacts, but less than did ATB subjects. Frequencies of IFNγ-producing CD4+ and CD8+ T cells induced by proteins Rv2659c and Rv1738 were higher in LTBI than ATB individuals. The results indicated that LTBI group in a high TB burden country demonstrated cell-mediated immune response to proteins Rv2659c and Rv1738 stronger than those of ATB. These immune responses likely contribute to natural protection against dormant M. tb and might be potential targets for a multi-stage TB vaccine.
Comparative analysis and improved design of LLC inverters for induction heating
Authors: Esteve Vicente , Jordán José , Dede Enrique J. , Martinez Pedro J. , Ferrara Kevil J. , Bellido Juan L. 
Published: April 2023

AbstractThis work presents a comparative analysis and design procedure of a converter based on an LLC resonant inverter used for induction heating applications depending on the transistor technology used and the selected operating frequency. A major objective of the work is to determinate the inverter design requirements leading to the optimal sizing of the components, improving its operation and minimizing power losses. Additionally, it proposes a design to obtain a great insensitivity of the inverter operation against large variations of load impedance. This article analyzes the operation of the inverter where the power regulation is carried out by phase‐shift (PS) modulation. Simple rules are formulated for the design of the resonant circuit components. The design has been verified testing a 25‐kW inverter in an industrial application of induction hardening operating at 500 kHz. Inverter efficiency is around 98.5% when using Silicon Carbide (SiC) MOSFETs.
A time‐scale adaptive a forecasting and dispatching integration strategy of the combined heat and power system considering thermal inertia
Authors: Wang Xiaojing , Han Li , Li Mengjie , Lu Panpan 
Published: April 2023

AbstractWith the integration of wind, electricity and heat, the combined heat and power system has the characteristic of multi‐time scales, in which the wind power has high uncertainty, the thermal system is difficult to synchronize with the power system for the large inertia and delay. Therefore, the fixed time‐scale dispatching is unable to guarantee the reliability of the system. Given the above problems, this paper proposes a time‐scale adaptive dispatching strategy of the combined heat and power system considering thermal inertia. This strategy analyzes the time characteristics of wind power forecasting error and the thermal inertia, while later establishing time‐scale adaptive dispatching sub‐main model of combined heat and power system. Specifically, in the time‐scale adaptive method, the system available reserve model is improved due to the power support of the thermal inertia. Based on the existing fixed time‐scale dispatching, this method dynamically adjusts the steps of forecasting and the time‐scales of dispatching when the system available reserve is inadequate to compensate for the wind power forecasting error. Taking the 4‐h fixed time‐scale dispatching as an example, the simulation results suggest that the proposed strategy achieves the dynamic dispatching of the combined heat and power system, while improving its reliability and stability.
Foreign Body Accidents in a Pediatric Emergency Department
Authors: Bohadana Saramira Cardoso , Santos Rayza Gaspar dos , Magalhães Mirella Kalyne Cavalcante , Cesar Regina Grigolli 
Published: April 2023

Abstract
Introduction Foreign body accidents (FBAs) are frequent in children and can be severe, being a common cause of morbidity and mortality and a public health problem. As these accidents are multifactorial, their cause can only be determined by analyzing the clinical details and characteristics of the object. Knowing the associated mechanisms and factors is essential to determine a risk profile and have a preventive, therapeutic, and diagnostic purpose.
Objective The present study aimed to describe the incidence of FBAs in otorhinolaryngology according to their anatomical location, focusing on the aerodigestive system.
Methods This is a retrospective study performed by reviewing the medical records of 668 cases seen in the emergency department of a tertiary pediatric hospital in São Paulo, state of São Paulo, Brazil, between 2014 and 2017.
Results Foreign bodies (FBs) were found in the digestive system (238/668), in the nasal cavities (206/668), in the ears (182/668), in the oropharynx (34/668), and in the respiratory system (8/668). A total of 91.77% of the patients were treated in the emergency room. The main age group affected was < 5 years old, with no difference between genders. The most frequent complications affected the digestive system and the most serious occurred in cases involving the respiratory system.
Conclusion Multidisciplinary teams should be ready in the emergency room to provide adequate care in FBAs. Early diagnosis, FB removal in the emergency room or the surgical center and follow-up are essential. Developing prevention campaigns including a risk profile for certain products and/or materials, helping to ensure safety for consumers, is necessary. For this, a national database with compulsory notification containing relevant information on FBAs in the pediatric population should be created.
Inpatient Decongestive Therapy for Lymphedema in Acute Postsurgical Head and Neck Cancer Patients*
Authors: Lemoine John Christian , Vaitaitis Vilija , Jarreau Trisha , Germain Joel St. , Son Leslie , Pou Anna M. , Mays Ashley 
Published: April 2023

Abstract
Introduction Head and neck lymphedema is an omnipresent morbidity related to head and neck cancer therapies. Studies on therapy for these patients in the acute postsurgical population have not been published to date.
Objective To assess changes in the measurements of lymphedema in surgical head and neck cancer patients during the hospital stay with implementation of modified decongestive therapy (MDT).
Methods Patients aged > 18 years undergoing neck dissection with or without primary-site resection or laryngectomy between 2016 and 2019 were included. Facial measurements were obtained prior to beginning MDT and again prior to discharge. A total facial composite measurement was calculated and used to assess change over time. Rates ≥ 2% of change were considered significant.
Results A total of 38 patients were included (subsites: larynx = 27; thyroid = 4; oral cavity = 3; and neck = 4). The mean number of days between surgery and the start of lymphedema therapy was 3.0 days. The mean number of days between measurements was 5.2 days. Reduction in the total composite score was observed in 37 (97%) patients, and 35 (92%) patients had a total composite reduction score > 2%. Tumor subsite and surgery type did not portend toward greater percent change, except for those patients treated with total laryngectomy, regional flap reconstruction, and neck dissection (p = 0.02).
Conclusion Acute postsurgical inpatient MDT was associated with reduced total composite measurements in patients after head and neck surgery. As the first published study on lymphedema therapy in this acute postsurgical period, further prospective case-control studies are warranted to explore further benefits of acute therapy.
PET/MRI Applications in Pediatric Epilepsy
Authors: Pedersen Christian , Aboian Mariam , Messina Steven A. , Daldrup-Link Heike , Franceschi Ana M. 
Published: April 2023

AbstractEpilepsy neuroimaging assessment requires exceptional anatomic detail, physiologic and metabolic information. Magnetic resonance (MR) protocols are often time-consuming necessitating sedation and positron emission tomography (PET)/computed tomography (CT) comes with a significant radiation dose. Hybrid PET/MRI protocols allow for exquisite assessment of brain anatomy and structural abnormalities, in addition to metabolic information in a single, convenient imaging session, which limits radiation dose, sedation time, and sedation events. Brain PET/MRI has proven especially useful for accurate localization of epileptogenic zones in pediatric seizure cases, providing critical additional information and guiding surgical decision making in medically refractory cases. Accurate localization of seizure focus is necessary to limit the extent of the surgical resection, preserve healthy brain tissue, and achieve seizure control. This review provides a systematic overview with illustrative examples demonstrating the applications and diagnostic utility of PET/MRI in pediatric epilepsy.
The Value of Multiple Medication Trials Prior to Balloon Sinuplasty in the Management of Chronic Sinonasal Disease
Authors: Shah Anand G. , Ojeaga Macaulay , Novak Josephine , Honrubia Vincent 
Published: April 2023

Abstract
Introduction In patients with chronic rhinosinusitis, conservative interventions with extended medical trials are often attempted prior to procedural treatment. Balloon sinuplasty (BSP) is an established procedure for symptomatic relief from chronic rhinosinusitis. However, data suggesting the suboptimal efficacy of prolonged medication management trials, prior to BSP, is lacking.
Objectives The purpose of this study was to evaluate the efficacy of prolonged medication management trials, prior to BSP, for patients with chronic rhinosinusitis.
Methods A retrospective review was performed for all adults with chronic rhinosinusitis who received extended medical management prior to their BSP at two outpatient clinics, from November 1, 2013, to June 31, 2018. The patients' Sino-Nasal Outcome Test (SNOT) scores were compared between baseline, post-medication trials, and post-BSP.
Results The SNOT scores of a total of 64 patients were collected. Overall, patients showed a significant worsening of symptoms during the medication management trials from baseline (p = 0.002126) but significant improvement of symptoms after undergoing BSP (p < 0.0001).
Conclusion The patient symptom burden worsened and prolonged during medication management trials. The BSP procedure alone showed significant improvement in the quality of life for chronic rhinosinusitis patients, when considering their SNOT scores. The worsening of patients' symptoms during medication management may invalidate the necessity of prolonged medication management trials.
Theoretical study of the spectroscopic constants of the ground state of the diatomic Ba-RG (RG = Kr, Xe, Rn) based on the coupled cluster theory with spin–orbit coupling
Authors: Xian Wei-Qi , Zhang Zhi-Peng , Tu Zhe-Yan , Zhou Hu , Li Lian-Bi , Chen Ai-Min 
Published: April 2023

Abstract
The spectroscopic constants including equilibrium distance, harmonic frequency and binding energy of the ground state of the diatomic Ba-RG (RG = Kr, Xe, Rn) are studied by using the closed-shell coupled-cluster theory with spin–orbit coupling (SOC) at the singles, doubles, and non-iterative triples level [CCSD(T)] based on the two-component relativistic pseudo-potentials. The advantage of the adopted computational protocol is that the SOC is incorporated in the post-Hartree–Fock part (i.e. the couple-cluster iteration) which makes it possible to significantly improve the computational efficiency. The extrapolation to the complete basis set (CBS) limit is used to provide the most accurate computational values in the framework of the adopted theoretical approach. The computational values to the CBS limit show that the SOC effect decreases the equilibrium distance by 0.067 Å while the binding energy increases by 21.023 cm−1 for the heaviest Ba-Rn, but not significant in the Ba-Kr and Ba-Xe. To date, both experimental and theoretical spectroscopic constants for Ba-Rn are unavailable, the present work thus provides the reliable theoretical results of the ground state of Ba-Rn for the future investigations.