bluuebunny/crossref_metadata_2025_split huggingface dataset:
Prevention of cleft lip and/or palate in A/J mice by licorice solution
Authors: Chimedtseren Ichinnorov , Niimi Teruyuki , Inoue Makoto , Furukawa Hiroo , Imura Hideto , Minami Katsuhiro , Garidkhuu Ariuntuul , Gantugs Anar‐Erdene , Natsume Nagato 
Published: June 2023

AbstractCleft lip and/or palate anomalies (CL ± P) are the most frequent birth defects affecting the orofacial region in humans. Although their etiology remains unclear, the involvement of environmental and genetic risk factors is known. This observational study aimed to investigate how the use of crude drugs with estrogen activity influenced an animal model's ability to prevent CL ± P. A/J mice were randomly divided into six experimental groups. Five of these groups consumed a drink containing crude drug licorice root extract, with the following weights attributed to each group: 3 g in group I, 6 g in group II, 7.5 g in group III, 9 g in group IV, and 12 g in group V, whereas a control group consumed tap water. The effect of licorice extract was examined for fetal mortality and fetal orofacial cleft development compared to the control group. The rates for fetal mortality were 11.28%, 7.41%, 9.18%, 4.94%, and 7.90% in groups I, II, III, IV, and V, respectively, compared to 13.51% in the control group. There were no significant differences in the mean weight of alive fetuses in all five groups compared to the control group (0.63 ± 0.12). Group IV showed the lowest orafacial cleft occurrence of 3.20% (8 fetuses) with statistical significance (p = 0.0048) out of 268 live fetuses, whereas the control group had the occurrence of 8.75% (42 fetuses) among 480 live fetuses. Our study showed that the dried licorice root extract may reduce orofacial birth defects in experimental animal studies.
Effects of crown contour on artificial biofilm removal efficacy with interdental cleaning aids: An in vitro study
Authors: Sirinirund Benyapha , Siqueira Rafael , Li Junying , Mendonça Gustavo , Zalucha Janet , Wang Hom‐Lay 
Published: June 2023

AbstractObjectivesTo evaluate the efficacy of various interdental cleaning aids for artificial biofilm removal on different implant‐supported crown designs.MethodsMandibular models with missing first molar were fabricated and installed with single implant analogs and loaded with crowns of different designs (concave, straight, and convex). Artificial biofilm was made with occlusion spray. Thirty volunteers (periodontists, dental hygienists, and laypersons) were asked to clean the interproximal areas. The crowns were unscrewed and photographed in a standardized setting. The outcome was measured by the cleaning ratio which represents the cleaned surfaces in relation to the area of the tested surface.ResultsA significant difference in favor of concave crown (p < .001) on the basal surface was cleaned by all tools, except the water flosser. There was evidence of an overall effect of “cleaning tool,” “surface,” and “crown design” (p < .0001) except for the “participant” factor. The mean cleaning ratio for each cleaning tool and overall combined surfaces were (in%): dental floss: 43.02 ± 23.93, superfloss: 42.51 ± 25.92, electric interspace brush: 36.21 ± 18.78, interdental brush: 29.10 ± 15.95, and electric water flosser: 9.72 ± 8.14. Dental floss and superfloss were significantly better (p < .05) than other tools in removing plaque.ConclusionsConcave crown contour had the greatest artificial biofilm removal, followed by straight and convex crowns at the basal surface. Dental floss and superfloss were the most effective interdental cleaning devices for artificial biofilm removal. None of the tested cleaning devices were able to completely remove the artificial biofilm from the interproximal/basal surfaces.
Single‐cellRNA‐sequencing analysis reveals enhanced non‐canonical neurotrophic factor signaling in the subacute phase of traumatic brain injury
Authors: Qiu Xuecheng , Guo Yaling , Liu Ming‐Feng , Zhang Bingge , Li Jingzhen , Wei Jian‐Feng , Li Meng 
Published: June 2023

AbstractBackgroundTraumatic brain injury (TBI) is a leading cause of long‐term disability in young adults and induces complex neuropathological processes. Cellular autonomous and intercellular changes during the subacute phase contribute substantially to the neuropathology of TBI. However, the underlying mechanisms remain elusive. In this study, we explored the dysregulated cellular signaling during the subacute phase of TBI.MethodsSingle‐cell RNA‐sequencing data (GSE160763) of TBI were analyzed to explore the cell–cell communication in the subacute phase of TBI. Upregulated neurotrophic factor signaling was validated in a mouse model of TBI. Primary cell cultures and cell lines were used as in vitro models to examine the potential mechanisms affecting signaling.ResultsSingle‐cell RNA‐sequencing analysis revealed that microglia and astrocytes were the most affected cells during the subacute phase of TBI. Cell–cell communication analysis demonstrated that signaling mediated by the non‐canonical neurotrophic factors midkine (MDK), pleiotrophin (PTN), and prosaposin (PSAP) in the microglia/astrocytes was upregulated in the subacute phase of TBI. Time‐course profiling showed that MDK, PTN, and PSAP expression was primarily upregulated in the subacute phase of TBI, and astrocytes were the major source of MDK and PTN after TBI. In vitro studies revealed that the expression of MDK, PTN, and PSAP in astrocytes was enhanced by activated microglia. Moreover, MDK and PTN promoted the proliferation of neural progenitors derived from human‐induced pluripotent stem cells (iPSCs) and neurite growth in iPSC‐derived neurons, whereas PSAP exclusively stimulated neurite growth.ConclusionThe non‐canonical neurotrophic factors MDK, PTN, and PSAP were upregulated in the subacute phase of TBI and played a crucial role in neuroregeneration.
Comprehensive analyses of brain cell communications based on multiple scRNA‐seq and snRNA‐seq datasets for revealing novel mechanism in neurodegenerative diseases
Authors: Zhang Chunlong , Tan Guiyuan , Zhang Yuxi , Zhong Xiaoling , Zhao Ziyan , Peng Yunyi , Cheng Qian , Xue Ke , Xu Yanjun , Li Xia , Li Feng , Zhang Yunpeng 
Published: June 2023

AbstractAimsComplex cellular communications between glial cells and neurons are critical for brain normal function and disorders, and single‐cell level RNA‐sequencing datasets display more advantages for analyzing cell communications. Therefore, it is necessary to systematically explore brain cell communications when considering factors such as sex and brain region.MethodsWe extracted a total of 1,039,459 cells derived from 28 brain single‐cell RNA‐sequencing (scRNA‐seq) or single‐nucleus RNA‐sequencing (snRNA‐seq) datasets from the GEO database, including 12 human and 16 mouse datasets. These datasets were further divided into 71 new sub‐datasets when considering disease, sex, and region conditions. In the meanwhile, we integrated four methods to evaluate ligand–receptor interaction score among six major brain cell types (microglia, neuron, astrocyte, oligodendrocyte, OPC, and endothelial cell).ResultsFor Alzheimer's disease (AD), disease‐specific ligand–receptor pairs when compared with normal sub‐datasets, such as SEMA4A‐NRP1, were identified. Furthermore, we explored the sex‐ and region‐specific cell communications and identified that WNT5A‐ROR1 among microglia cells displayed close communications in male, and SPP1‐ITGAV displayed close communications in the meninges region from microglia to neurons. Furthermore, based on the AD‐specific cell communications, we constructed a model for AD early prediction and confirmed the predictive performance using multiple independent datasets. Finally, we developed an online platform for researchers to explore brain condition‐specific cell communications.ConclusionThis research provided a comprehensive study to explore brain cell communications, which could reveal novel biological mechanisms involved in normal brain function and neurodegenerative diseases such as AD.
A preliminary transcriptomic analysis of the orbitofrontal cortex of antisocial individuals
Authors: Piras Ignazio S. , Braccagni Giulia , Huentelman Matthew J. , Bortolato Marco 
Published: June 2023

AbstractAimsAntisocial personality disorder (ASPD) and conduct disorder (CD) are characterized by a persistent pattern of violations of societal norms and others' rights. Ample evidence shows that the pathophysiology of these disorders is contributed by orbitofrontal cortex (OFC) alterations, yet the underlying molecular mechanisms remain elusive. To address this knowledge gap, we performed the first‐ever RNA sequencing study of postmortem OFC samples from subjects with a lifetime diagnosis of ASPD and/or CD.MethodsThe transcriptomic profiles of OFC samples from subjects with ASPD and/or CD were compared to those of unaffected age‐matched controls (n = 9/group).ResultsThe OFC of ASPD/CD‐affected subjects displayed significant differences in the expression of 328 genes. Further gene‐ontology analyses revealed an extensive downregulation of excitatory neuron transcripts and upregulation of astrocyte transcripts. These alterations were paralleled by significant modifications in synaptic regulation and glutamatergic neurotransmission pathways.ConclusionThese preliminary findings suggest that ASPD and CD feature a complex array of functional deficits in the pyramidal neurons and astrocytes of the OFC. In turn, these aberrances may contribute to the reduced OFC connectivity observed in antisocial subjects. Future analyses on larger cohorts are needed to validate these results.
USP14 inhibition promotes recovery by protecting BBB integrity and attenuating neuroinflammation in MCAO mice
Authors: Hou Wenzhong , Yao Jianping , Liu Junjie , Lin Xiaohong , Wei JueXian , Yin Xiaofan , Huang Hongbiao , Chen Xiaohui , Yang Guo‐Yuan , He Xiaosong 
Published: June 2023

AbstractAimBlood–brain barrier (BBB) dysfunction is one of the hallmarks of ischemic stroke. USP14 has been reported to play a detrimental role in ischemic brain injury. However, the role of USP14 in BBB dysfunction after ischemic stroke is unclear.MethodsIn this study, we tested the role of USP14 in disrupting BBB integrity after ischemic stroke. The USP14‐specific inhibitor IU1 was injected into middle cerebral artery occlusion (MCAO) mice once a day. The Evans blue (EB) assay and IgG staining were used to assess BBB leakage 3 days after MCAO. FITC‐detran test was slected to examine the BBB leakage in vitro. Behavior tests were conducted to evaluate recovery from ischemic stroke.ResultsMiddle cerebral artery occlusion increased endothelial cell USP14 expression in the brain. Furthermore, the EB assay and IgG staining showed that USP14 inhibition through IU1 injection protected against BBB leakage after MCAO. Analysis of protein expression revealed a reduction in the inflammatory response and chemokine release after IU1 treatment. In addition, IU1 treatment was found to rescue neuronal loss resulting from ischemic stroke. Behavior tests showed a positive effect of IU1 in attenuating brain injury and improving motor function recovery. In vitro study showed that IU1 treatment could alleviate endothelial cell leakage induced by OGD in cultured bend.3 cells through modulating ZO‐1 expression.ConclusionsOur results demonstrate a role for USP14 in disrupting the integrity of the BBB and promoting neuroinflammation after MCAO.
Association of stigma, diabetes distress and self‐efficacy with quality of life in adolescents with type 1 diabetes preparing to transition to adult care
Authors: Soufi Arij , Mok Elise , Henderson Mélanie , Dasgupta Kaberi , Rahme Elham , Nakhla Meranda 
Published: June 2023

AbstractAimsIn type 1 diabetes (T1D), psychosocial factors may impact quality of life (QOL) and clinical outcomes, but remain understudied, particularly during late adolescence. Our aim was to determine whether stigma, diabetes distress and self‐efficacy are associated with QOL in adolescents with T1D as they are preparing to transition to adult care.MethodsWe conducted a cross‐sectional study of adolescents (ages 16–17 years) with T1D participating in the Group Education Trial to Improve Transition (GET‐IT) in Montreal, Canada. Participants completed validated questionnaires on stigma using the Barriers to Diabetes Adherence (BDA) stigma subscale, self‐efficacy (Self‐Efficacy for Diabetes Self‐Management Measure [SEDM], score 1–10), diabetes distress (Diabetes Distress Scale for Adults with type 1 diabetes) and QOL (Pediatric Quality of Life Inventory [PedsQL] 4.0 Generic Core Scale and PedsQL 3.2 Diabetes Module). We examined associations of stigma, diabetes distress and self‐efficacy with QOL using multivariate linear regression models adjusted for sex, diabetes duration, socioeconomic status and HbA1c.ResultsOf 128 adolescents with T1D, 76 (59%) self‐reported having the diabetes‐related stigma and 29 (22.7%) reported experiencing diabetes distress. Those with stigma had lower diabetes‐specific and general QOL scores compared with those without stigma, and stigma and diabetes distress were both associated with lower diabetes‐specific QOL and lower general QOL. Self‐efficacy was associated with higher diabetes‐specific and general QOL.ConclusionsStigma and diabetes distress are associated with lower QOL, whereas self‐efficacy is associated with higher QOL in adolescents with T1D preparing to transfer to adult care.
Effect of four plant extracts on the mortality, population growth, and fluctuating asymmetry of Sitophilus oryzae
Authors: Ačanski Jelena , Gvozdenac Sonja , Radenković Marko , Prvulović Dejan , Tanasković Snežana , Horvatović Mladen 
Published: June 2023

AbstractManagement of storage pest insects relies heavily on chemical control, and there is a need to develop more sustainable management practices. Here, we evaluated the impact of 2% ethanol plant extracts of Ajuga reptans L., Ajuga pyramidalis L. (both Lamiaceae), Urtica dioica L. (Urticaceae), and Cannabis sativa L. (Cannabaceae) plants on mortality, population growth, and developmental stability [measuring fluctuating asymmetry (FA)] of the rice weevil, Sitophilus oryzae (L.) (Coleoptera: Curculionidae), a worldwide stored product pest. FA refers to small, random deviations occurring between the left and right sides of bilaterally symmetrical organisms; these deviations increase in response to environmental stress, making FA a reliable method to measure the impact of stress. FA was measured by means of geometric morphometrics, a method that allows for analyzing the whole landmark configuration of the insect, rather than taking single measurements. Extracts of the mentioned plants were used to treat maize (Zea mays L., Poaceae) kernels on which experimental populations of the rice weevil were grown, and we assessed mortality after 24–72 h, population growth after 30–90 days, and developmental stability after 90 days. Screening bioassays showed that S. oryzae adults were most affected by Ajuga extracts; Ajuga spp., especially A. reptans, significantly reduced population growth. In concordance, Ajuga extracts increased FA. The effects of U. dioica and C. sativa extract were less pronounced. None of the extracts significantly affected insect mortality. Overall, it can be concluded that A. reptans and A. pyramidalis are potential sources of bioactive compounds that may be further used for S. oryzae control. The results obtained confirm that variation in body shape asymmetry can be used as an indicator of population disturbance when insects are exposed to different types of stressors.
Random fatigue life analysis of carbon fiber‐reinforced plastic for automotive drive shaft with environment temperature data
Authors: Yang Xizhou , Liu Xintian , Wang Jinbo 
Published: June 2023

AbstractAutomotive drive shaft is an indispensable key component of the transmission system, and the use of composite drive shafts has shown great promise. The epoxy matrix of the shaft is susceptible to the effects of temperature in comparison to carbon fiber. To investigate the influence of the environmental temperature on the fatigue life of a carbon fiber‐reinforced plastic drive shaft, a temperature‐based property curve and performance fraction model for the shaft are established. The temperature coefficient is calculated and introduced by combining the collected temperature data with the property fraction function. A generalized S‐N surface of the composite drive shaft is proposed based on experimental data. The fatigue life under collected and processed random loads of the carbon fiber‐reinforced plastic drive shaft is predicted by modified Miner's law. The effect of mean, amplitude of load and ambient temperature on fatigue life are taken into account.
Pre‐medical assessment of root–canal–filled teeth with asymptomatic apical periodontitis—A multifaceted balancing act
Authors: Olsson Jenny , Wolf Eva , Ljunggren Anna 
Published: June 2023

AbstractAimPrior to certain medical therapies, dental assessment and treatment of oral foci of infection are recommended. The aim of the present study was to acquire a deeper understanding of the decision‐making process regarding the pre‐medical management of root–canal–filled teeth with asymptomatic apical periodontitis (AAP).MethodologyHospital‐affiliated dentists in Sweden were contacted for a semi‐structured, in‐depth interview. The absolute inclusion criterion was that the dentists had experienced and could recount at least two authentic cases involving root–canal–filled teeth with AAP—one case having resulted in pre‐medical treatment, and one having resulted in expectancy. Fourteen interviews, with fourteen informants, were conducted and included in the study. During the interviews, open‐ended questions and comments encouraging the informants to elaborate and clarify their experiences were offered. The interviews were digitally recorded, transcribed verbatim and analysed using Qualitative Content Analysis with an inductive approach.ResultsA theme describing the latent content was identified through interpretation of the collected data: A multifaceted balancing act characterized by a sometimes‐difficult risk–benefit‐estimation, where an increased uncertainty entails an increased reliance on external opinions. Three main categories, comprising four sub‐categories, describing the manifest content were recognized: The tipping scale, The team effort and The frame of reference.ConclusionsThe current interview study found pre‐medical decision‐making regarding root–canal–filled teeth with AAP to be a multifactorial and contextual process marked by uncertainty and collaborative measures. Further research, resulting in the development of evidence‐based treatment guidelines, is suggested necessary.
Building professional holding environments for crowd work job crafting through online communities
Authors: Strunk Kim Simon , Strich Franz 
Published: June 2023

AbstractWork is increasingly being organised via online platforms outside guiding organisational structures. Instead of having colleagues at work, crowd workers connect in online communities. We investigate how crowd workers build professional holding environments in online communities to compensate for the lack of organisational structures and we consider how they craft their crowd work activities to enhance their work experience and reduce its long‐term precarity. Following a qualitative research design, this paper uses 675 forum interactions collected across six online communities. Based on our findings, we propose the concept of professional holding environments and provide a model for building such holding environments and job crafting in online communities. We thereby expand previous research on holding environments comprised of family members and friends by revealing the impact of professional online communities and their role in professionalisation and crafting supportive social structures in online crowd work.
Lifting Stress from the Day: A Women's Well‐Being Online Community Art Project
Authors: Tutchell Suzy 
Published: June 2023

AbstractThis article tells the story of a university community engagement project that began in the late spring of 2020 when the world went into lockdown. Increased concern over women's welfare and well‐being was brought into question in relation to those who are vulnerable with complex needs and had suffered societal‐induced hardships. In conjunction with an innovative women's community project, this study considered the value of art‐based online support as a platform from which to offer a non‐judgemental and mindful space with a focus on identity and empowerment. The study is based on weekly art drop‐in online workshops for vulnerable women and considers the value of hybrid ‘spaces’ as a pedagogical and practical model of communicative support and collective care. Findings from this community‐engaged project highlight the responses and shifts within subjectivities through situated art experiences. As art educators, it offers us an understanding of the potential for cumulative and affective artmaking to build communities of care as an inclusive and emancipatory learning tool.
Transcriptomic analyses revealed detoxification genes responsive to solanine treatment in Phthorimaea operculella
Authors: Yan Han , Dong Wenbo , Wang Yuquan , Ning Hengheng , Gao Yulin , Li Fen , Wu Shaoying 
Published: June 2023

AbstractPhthorimaea operculella attacks potatoes worldwide, causing great losses in potatoes' quantity and quality. P. operculella feed on potatoes with a relatively high concentration of steroidal glycoalkaloids called solanine, which potatoes produce as secondary metabolites. The potential genes involved in solanine‐induced response in P. operculella were identified by performing next‐generation sequencing and Illumina RNA‐seq analysis on solanine‐treated P. operculella to explore differentially expressed genes. Four major families of detoxification enzymes were identified, including 71 cytochrome P450 monooxygenases (CYPs), 22 glutathione S‐transferases (GSTs), 58 esterases (ESTs) and 16 UDP‐glucuronosyltransferases (UGTs). The Illumina RNA‐Seq data showed 2875 and 1625 differentially expressed genes (DEGs) from the P. operculella larvae after exposure to the LC25 and LC75 of solanine compared with the untreated controls. Furthermore, 21 upregulated DEGs, including 13 CYPs, 3 GSTs, 4 ESTs and 1 UGT, and 20 downregulated DEGs, including 5 CYPs, 3 GSTs, 9 ESTs and 3 UGTs, were detoxification genes that may be related to solanine‐induced response and needed to be further verified. The RT‐qPCR results among the six upregulated P450 unigenes after solanine treatment were consistent with RNA‐Seq data, confirming the accuracy and reproducibility of the gene expression profiles generated from RNA‐seq. This study provides new information about the detoxification genes involved in solanine‐induced response in P. operculella.