bluuebunny/crossref_metadata_2025_split huggingface dataset:
Pregnancy and the Risk for Cancer in Neurofibromatosis 1
Authors: Kallionpää Roope A. , Määttänen Juha , Leppävirta Jussi , Peltonen Sirkku , Peltonen Juha 
Published: February 2025

ABSTRACTBackgroundNeurofibromatosis type 1 (NF1) is associated with a high risk for cancer. Benign cutaneous neurofibromas of women with NF1 may increase in size and number during pregnancy. However, it is not known whether pregnancy affects the risk for cancer in NF1.MethodsWe retrieved the pregnancies of women in the Finnish NF1 cohort and in a 10‐fold control cohort from the Finnish Medical Birth Register. Cancers occurring during or after pregnancy were obtained from the Finnish Cancer Registry and summarized using standardized incidence ratio (SIR). The cancer incidence of nonNF1 mothers of individuals with NF1 was also estimated.ResultsTotals of 263 pregnancies in 136 women with NF1 and 3176 pregnancies in 1720 controls were observed. In the NF1 group, two cancers were identified during pregnancy and the year following the delivery (SIR 6.44, 95% CI 1.07–19.89). Among controls, the SIR was markedly lower (0.25, 95% CI 0.01–1.08). Within 1–10 years after pregnancy, the SIR of women with NF1 was 7.54 (95% CI 4.15–12.41). The SIR of women with NF1 aged 20–49 years, and without a known history of deliveries was 8.63 (95% CI 6.08–11.81). The nonNF1 mothers displayed a SIR of 0.81 (95% CI 0.66–1.00) after giving birth to a child with NF1.ConclusionsThe pregnancy‐related cancer incidence in women with NF1 is similar to women with NF1 aged 20–49 years overall, although notably higher than in the general population. Giving birth to a child with NF1 does not affect the risk for cancer in women without NF1.
Thermoresponsive Nanocarriers Transduced by Inorganic Nanoparticles: Design Considerations and Applications in Drug Delivery
Authors: Nikoletić Anamarija , Maleković Mirela , Kozalak Gül , Palivan Cornelia G. , Tagit Oya 
Published: February 2025

AbstractThermoresponsive polymers, which undergo phase transitions within physiologically tolerated temperatures, are key to developing drug delivery systems (DDS) with precise spatial and temporal control, potentially addressing challenges associated with the treatment of complex diseases. Inorganic nanoparticles with unique optical, electronic, and magnetic properties serve as efficient transducers, converting external stimuli into localized heat to trigger thermoresponsive nanocarriers. This review explores the design and application of thermoresponsive nanocarriers transduced by inorganic nanoparticles as DDS. Following a brief description of temperature‐triggered phase transition of polymers and heat generation mechanisms by inorganic nanoparticles, strategies to integrate these components into hybrid systems are described. Examples demonstrating the utility of these hybrid systems as advanced DDS are discussed, highlighting their potential for precise drug release alongside theranostic capabilities by combining therapy with imaging. Despite the challenges in design, synthesis, and biological applications, thermoresponsive polymer‐inorganic hybrids hold immense promise for transforming drug delivery and biomedical innovations.
Transition to Agroecological Practices: Positive Effects on Streams and Biofilms
Authors: Mujica Micaela A. , De Gerónimo Eduardo , Gelis María Mercedes Nicolosi , Pecile Maria Agostina , del Puerto Juan Martin Paredes , Mosca Agustin , Fanelli Silvia L. , Jensen Roberto , Cordero Tomas , Paracampo Ariel H. , Cochero Joaquin 
Published: February 2025

ABSTRACTTransformations in agricultural practices, particularly regarding chemical inputs such as pesticides, affect physical and chemical characteristics and biotic communities of streams. Currently, many conventional production systems are transitioning to economically and environmentally more sustainable production, which aims to reduce the use of synthetic inputs. However, it remains unclear whether agroecological practices positively impact the environment. Here, we compared physical and chemical characteristics and the toxicity of sediments from streams in areas of conventional and agroecological transition horticultural practices as well as reference areas. Additionally, the effects of horticultural practices on stream biofilms were assessed in 7‐day bioassays, measuring chlorophyll‐a, extracellular polymeric substance concentrations, ash‐free dry weight, and diatom motility. In areas with conventional horticulture, pesticides were more frequent and had higher concentrations than in areas with agroecological horticulture. This was reflected in biofilms, with lower algal biomass and higher diatom cell motility under conventional horticulture. Our study suggested that the transition to agroecological practices, even in the early stages, had positive effects on aquatic ecosystems, and we found similarities in pesticide use and biological characteristics between sites with agroecological horticulture and less‐impact sites, for example, grasslands sites. This study reports and integrates the effects of a complex mixture of contaminants used in intensive horticulture, providing a more realistic assessment of their environmental consequences.
Assessment of the Cytotoxicity Mechanism of Diazinon on HFFF2 Cells: A Bioinformatic and Experimental Study
Authors: Sarailoo Mehdi , Asghariazar Vahid , Seifimansour Sina , Kadkhodayi Mahtab , Zare Erfan , Vajdi Parnia , Vostakolaei Mehdi Asghari 
Published: February 2025

ABSTRACTPesticide exposure can cause many skin diseases such as hypopigmentation and contact dermatitis, but the underlying mechanisms remain unclear. Furthermore, Organophosphate pesticides (OPs) including Diazinon (DZN) can affect cellular pathways like ATPase, leading to mitochondrial energy deficit and even apoptosis in the cell's functions. Following cell exposure to the OP pesticide DZN through treatment, we evaluated alteration in gene expression and DNA damage. Bioinformatic analysis was performed based on the AutoDock, Protein Data Bank, STRING, Way2Drug, and Comparative Toxicogenomics databases and tools. The MTT assay, wound healing, DAPI staining, flow cytometry, and real‐time PCR were applied in the current study. The results showed that the viability and migration capacity of HFFF2 cells decreased, and the apoptosis rate increased in the DZN‐treated group. These findings revealed that DZN regulated the expression of the apoptotic genes in DZN cells.
HOXA10‐AS Enhances Gastric Cancer Cell Proliferation, Migration, and Invasion via the p38 MAPK/STAT3 Signaling Pathway
Authors: Hu Yu , Zhang Ying , Ding Meng , Xu Ruisi 
Published: February 2025

ABSTRACTGastric cancer (GC) represents a major global health concern, with over 1 million new cases diagnosed annually worldwide. Emerging studies have highlighted the significant correlation between long noncoding RNAs (lncRNAs) and the progression of GC. The objective of the current study is to investigate the roles and mechanism of lncRNA homeobox A10 antisense RNA (HOXA10‐AS) in modulating malignant properties of GC cells. RT‐qPCR was employed to detect HOXA10‐AS expression in GC cells or human normal gastric epithelium cells. The cellular localization of HOXA10‐AS and mRNA HOXA10 were detected using RNA fractionation assays. Colony forming assays and Transwell assays were performed to assess the proliferative, invasive, and migratory capabilities of GC cells. Western blot analysis was used to determine protein levels of epithelial mesenchymal transition (EMT) markers in GC cells. RNA immunoprecipitation, RNA pulldown assays and luciferase assays were conducted to explore gene interaction. As shown by experimental results, HOXA10‐AS showed high expression in GC cells. The silencing of HOXA10‐AS led to weakened proliferative, invasive, and migratory abilities of GC cells, as well as inhibition of the EMT process. Moreover, HOXA10‐AS positively regulated HOXA10 expression by interacting with miR‐29a/b/c‐3p. Additionally, overexpression of HOXA10 counteracted the repressive impacts on malignant cellular process caused by the knockdown of HOXA10‐AS. Furthermore, HOXA10‐AS activated the p38 MAPK/STAT3 signaling pathway via upregulation of HOXA10. In conclusion, HOXA10‐AS upregulates HOXA10 expression through interaction with miR‐29a/b/c‐3p. The resultant increase in HOXA10 expression activates the p38 MAPK/STAT3 signaling, thereby promoting GC cell growth, migration, invasion, and EMT process.
Methyl Donor Ameliorates CCl4‐Induced Nephrotoxicity by Inhibiting Oxidative Stress, Inflammation, and Fibrosis Through the Attenuation of Kidney Injury Molecule 1 and Neutrophil Gelatinase‐Associated Lipocalin Expression
Authors: Manhar Nirmal , Singh Sumeet Kumar , Yadav Poonam , Bishnolia Manish , Khurana Amit , Bhatti Jasvinder Singh , Navik Umashanker 
Published: February 2025

ABSTRACTCarbon tetrachloride (CCl4), a volatile organic compound, is harmful to multi‐organs, including the liver, lungs, muscles, and kidneys. Methyl donors, such as methionine, choline, betaine, and folic acid, are vital to one‐carbon metabolism and have great potential to alleviate oxidative stress and inflammation, thus mitigating disease onset. Hence, the current study aims to examine the therapeutic effect of methyl donors against CCl4‐induced nephrotoxicity. Nephrotoxicity was developed in male Sprague Dawley rats using CCl4 at a dose of 1 mL/kg (4‐week model induction) twice a week via the intraperitoneal route. Thereafter, methyl donor treatments through oral gavage were given for the next 6 weeks with a continuation of CCl4 administration. Biochemical, oxidative stress parameters, histopathological, and qRT‐PCR analyses were done at the completion of the 10‐week. Biochemical analyses revealed that CCl4 induces nephrotoxicity, as evidenced by increased urea and creatinine levels and decreased albumin levels. These detrimental effects were significantly ameliorated by methyl donor treatment. Moreover, CCl4 decreased the antioxidant enzyme activity (superoxide dismutase; SOD and catalase; CAT) while increasing oxidative stress markers (malondialdehyde; MDA and nitrite). Methyl donor treatment effectively mitigated these oxidative changes. Histopathological analysis demonstrated the nephroprotective effect of methyl donors against CCl4‐induced nephrotoxicity, showing reduced tissue damage and protection of renal architecture. At the molecular level, methyl donor treatment alleviated the CCl4‐induced increase in kidney injury biomarkers (Kidney injury molecule 1; KIM‐1 and Neutrophil gelatinase‐associated lipocalin; NGAL), as well as inflammatory (IL‐6 and TNF‐α) and fibrosis‐related genes (Acta‐2 and TGF‐β). In conclusion, our findings suggest that methyl donors possess anti‐inflammatory and anti‐fibrotic properties. They protect against CCl4‐induced oxidative damage to renal cells, likely due to their reactive oxygen species scavenging capabilities and their ability to restore key early renal injury biomarkers (KIM‐1 and NGAL). Methyl donors hold great promise as a cutting‐edge therapy approach for preventing CCl4‐induced nephrotoxicity.
Low‐Power Blue LED Modulates NF‐κB and Proinflammatory Cytokines in Doxorubicin‐Treated MDA‐MB‐231 Cells
Authors: Fonseca Teixeira Adilson , Pires Bruno Ricardo Barreto , Panis Carolina , Monte‐Alto‐Costa Andréa , da Fonseca Adenilson de Souza , Mencalha Andre Luiz 
Published: February 2025

ABSTRACTDoxorubicin is a crucial chemotherapy used in the treatment of triple‐negative breast cancer (TNBC) patients, but elevated doxorubicin doses may induce therapeutic resistance. To overcome this limitation, we have previously established a photodynamic therapeutic (PDT)‐like strategy that irradiates doxorubicin‐treated cells with a low‐power nonionizing blue LED device. This combined treatment increases the production of reactive oxygen species to promote cell death, consequently enabling reduced doxorubicin dosages. Yet, precisely determining the molecular mechanisms that drive this outcome is still required for advancing such PDT‐like approach. Here, we aimed to correlate the expression of the inflammatory markers NF‐κB, IL‐8, IL‐6, and IL‐1β with the survival of TNBC cells submitted to our PDT‐like protocol. Our results show that NF‐κB/p65 nuclear levels were enhanced in MDA‐MB‐231 cells treated with doxorubicin and blue LED. Moreover, this PDT‐like strategy increased IL‐6 mRNA levels in MDA‐MB‐231 cells. IL‐1β and IL‐8 mRNA were upregulated in samples incubated with doxorubicin regardless of concomitant irradiation with blue LED. These results show that our PDT‐like protocol is effective in elevating inflammatory signals, shedding light on the molecular mechanisms that underlie the efficacy of this innovative anticancer therapeutic approach.
Decoding SARS‐CoV‐2 Inhibition: Insights From Molecular Dynamics Simulation of Condensed Amino Thiourea Scaffold Small Molecules
Authors: Shen Xiaoli , Zhang Hao , Zhang Pengyin , Zhao Xuerui , Liu Chang , Ju Jianan , Liu Aijun , Wang Song 
Published: February 2025

ABSTRACTThe main protease (Mpro) of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS‐CoV‐2) plays a crucial role in viral replication. In this study, the binding modes and inhibitory mechanisms of eight condensed amino thiourea scaffold inhibitors of Mpro in proteins were investigated using a combination of molecular docking, molecular dynamics simulations, and MM/PBSA binding free energy calculations. The results indicated that the para‐hydroxyl group on the benzene ring at the head of the inhibitor has a decisive influence on the initial docking pose and binding free energy strength of the inhibitor. Additionally, the position and length of the hydrophobic side chain on the tail six‐membered ring significantly impacted the final binding pose of the inhibitor. The presence of a long hydrophobic side chain in the ortho position of this ring, through its interaction with the P4 hydrophobic pocket, led to an opposite binding mode in the protein compared with when it was present with or without the para‐side chain. Different lengths of para‐substituted side chains affected the positioning of the inhibitors in the enzyme. These different binding modes led to variations in the binding free energy between the inhibitor and the protein, which in turn gave rise to differences in inhibitory capability.
A comprehensive review on progression and innovations in microwave assisted extraction technology for essential oils
Authors: Dhotre Irsha 
Published: February 2025

AbstractThis Review Paper provides a comprehensive analysis of extraction methods for essential oils, focusing particularly on the innovative Microwave Assisted Extraction (MAE) technology. Essential oils, derived from various plant parts, are highly valued for their aromatic and therapeutic properties, finding applications in industries such as perfumery, aromatherapy, cosmetics, and food flavoring. Traditional extraction methods often face challenges, including prolonged extraction times, excessive solvent usage, and the risk of degrading sensitive compounds. In contrast, MAE has emerged as a superior alternative, offering significant advantages such as expedited extraction processes, reduced solvent consumption, and enhanced preservation of volatile compounds. This study highlights key findings, including the optimal parameters for MAE, such as microwave power, extraction time, and solvent‐to‐raw material ratios, which collectively influence the yield and quality of essential oils. Notably, findings of this survey, indicates that MAE can achieve up to a 40% increase in essential oil yield compared to conventional methods. The review also discusses the evolution of MAE techniques, from initial simple heating methods to advanced systems incorporating infrared temperature sensors and magnetic stirrers, which improve precision and efficiency. By elucidating these advancements, this paper underscores the transformative impact of MAE on the extraction of essential oils, paving the way for future research and development in this field. © 2025 Society of Chemical Industry (SCI).
On the Fila Olfactoria and the Cribriform Region of the Crocodylia
Authors: Dille Matthew , Cramberg Michael , DeLeeuw Hadyn , Pick Emily , Thompson Mary , Young Bruce A. 
Published: February 2025

ABSTRACTIn mammals the fila olfactoria, fascicles of axons coursing from sensory neurons in the olfactory epithelium to the glomeruli of the olfactory bulb, not only have a topographic projection pattern but also serve as routes for cerebrospinal fluid (CSF) drainage from around the brain. Les is known about the fila olfactoria in nonmammalian taxa. This work explores the fila olfactoria of the American alligator (Alligator mississippiensis) using a combination of gross dissection, histology, Diffusible Iodine‐based contrast‐enhanced computed tomography, latex corrosion casting, and India ink tracers. In Crocodylians, as in other nonmammalian vertebrates, the fila olfactoria courses through a foramen in the nasal capsule rather than an ethmoidal cribriform plate. In Alligator this foramen is filled by dense connective tissue; prominent perineural spaces extend through the connective tissue, effectively making it perforate like the cribriform plate. Latex or India ink introduced into the cranial CSF pass through the dense connective to reach the submucosa of the olfactory epithelium, suggesting that Crocodylians have the same cranial CSF drainage pattern as mammals. In Alligator, the fila olfactoria is asymmetric, with more fascicles entering the ventral and lateral surfaces of the olfactory bulb than the dorsal or medial surfaces. If individual fascicles of the fila olfactoria are traced in Alligator, a clear topographic projection emerges; with medial and lateral polarity maintained between olfactory epithelium and olfactory bulb, and a rostral‐caudal polarity projecting as dorsal‐ventral on the olfactory bulb.
A Novel Forecasting Approach to Schedule Electric Vehicle Charging Using Real‐Time Data
Authors: Singh Arpana , Nangia Uma , Rizwan M. 
Published: February 2025

ABSTRACTThe rapid adoption of electric vehicle (EV) has increased the need for precise demand estimates to ensure grid stability, reduce operational costs, and strategically plan the expansion of charging stations. Existing forecasting approaches struggle to capture the complexity and change of the EV load patterns, especially over time. The effective development and optimization of the charging infrastructure are critically dependent on accurate EV load forecasting. This paper proposes a hybrid forecasting approach that combines long short‐term memory models with advanced decomposition methods like empirical mode decomposition, ensemble empirical mode decomposition, and complete ensemble empirical mode decomposition using adaptive noise and seasonal‐trend decomposition to address this challenge. The proposed framework is tested for 15, 30, 60, and 120 min to show its adaptability and robustness. Statistical evaluations show that decomposition approaches using long short‐term memory increase predicting accuracy across all time intervals. STL‐LSTM reduces the forecast error by 52.38% between hybrid methods. Kolmogorov–Smirnov, Shapiro–Wilk, and t‐tests confirm the results, improving consistency and dependability. This paper shows that hybrid decomposition‐based forecasting models can scale and accurately manage future EV charging demands, overcoming the limits of traditional techniques.
Metabolomic Profiling and Genome‐Wide Analysis of Bacillus subtilis NBAIR‐BSWG1 Reveals Cyclic Lipopeptides as Key Antagonists
Authors: Surendra Ankitha Karegowdru , Kumaraswamy Radha Tumkur , Seegenahalli Ruqiya , Kukreti Aditya , Manjunatha Lavanya Siddanahalli , Aravindaram Kandan , Gopalsamy Sivakumar , Syamala Ramya Ramesan , Channappa Manjunatha 
Published: February 2025

ABSTRACTUnderstanding bacterial genetics and metabolism is vital for developing biopesticides. This study investigates Bacillus subtilis NBAIR‐BSWG1, a strain well known for its antagonistic potential. Crude lipopeptides extracted from the strain were evaluated for in vitro activity, showing complete inhibition of Rhizoctonia solani at a concentration of 50 μL/mL potato dextrose agar. To delve deeper into its antagonistic mechanisms, we conducted whole‐genome sequencing of NBAIR‐BSWG1 using Illumina NextSeq 500. Subsequent analysis with the BlastX diamond tool revealed 19 key biosurfactant genes, including surfactin (srfAA, srfAC, srfAD, srfP), fengycin (ppsE, ppsD, ppsC, ppsB), and putisolvin (dnaK), which were further confirmed by PCR using specific primers. Meanwhile, antiSMASH analysis revealed gene clusters with 100% similarity to those responsible for the synthesis of fengycin, bacilaene, bacillibactin, subtilosin A, and bacilysin, as well as clusters with 82% similarity to surfactin synthesis genes. Additionally, liquid chromatography‐mass spectrometry was performed to analyze the cell‐free extract produced by NBAIR‐BSWG1, revealing the presence of various cyclic lipopeptides, including multiple peaks corresponding to surfactin, iturin, and several novel lipopeptide compounds. This study highlights B. subtilis NBAIR‐BSWG1 cyclic lipopeptides as a key to broad‐spectrum bio‐control and establishes the strain as highly potent.
Human Blood Investigation of Ta‐Coated 316L Stainless Steel to Evaluate Corrosion and Wear Behaviour Against Other Artificial Fluids for Orthopaedic Implants
Authors: Pathote Dileep , Ranjan Ashwani , Mandal Abhijit , Kumar Sandeep , Gautam R. K. , Behera C. K. 
Published: February 2025

ABSTRACTStainless steel (316L) is widely used in biomedical applications, but concerns persist regarding its long‐term surface properties, particularly in varying patient environments over time. This study aims to enhance the surface properties of 316L stainless steel through a 1.504 μm tantalum (Ta) coating applied via DC magnetron sputtering. The corrosion and wear behaviours were evaluated in different mediums, including human blood (HB), artificial saliva (AS), Ringer's solution (RS), and simulated body fluid (SBF). The Ta‐coated 316L SS exhibited a shift toward nobler corrosion behaviour, with a corrosion rate of 0.146 × 10−2 mm/year in human blood, compared to the uncoated 316L SS, which showed corrosion rates of 0.325, 0.556, 0.779, and 1.425 × 10−2 mm/year in HB, RS, AS, and SBF, respectively. Electrochemical impedance spectroscopy (EIS) results indicated higher corrosion resistance of Ta‐coated 316L SS in human blood than in other fluids. Potentiodynamic polarisation (PDP) results reveal that corrosion rate and current increased as the fluid environment shifted from human blood to simulated body fluid. Wear tests further demonstrated the superior wear resistance of the Ta‐coated 316L SS, with a minimum wear rate of 0.086 × 10−5 mm³/Nm, compared to the uncoated 316L SS, which exhibited a maximum wear rate of 0.25 × 10−5 mm³/Nm. The microstructural analysis confirmed significant improvements in the surface properties of Ta‐coated 316L SS, particularly in the human blood environment.