bluuebunny/crossref_metadata_2025_split huggingface dataset:
Unlocking the wealth of Dutch pollen data for future research and education
Authors: de Wolf Iris K. , Donders Timme H. , Hoek Wim Z. , Gouw-Bouman Marjolein T.I.J. , Giesecke Thomas 
Published: September 2023

AbstractNearly 100 years ago, scientists started analysing fossil pollen and spores in the Netherlands. Since then, an enormous number of fossil pollen datasets have been generated by several researchers at different research institutes. Until recently, most pollen data from the Netherlands was not publicly available. With this communication we like to advertise that this situation has changed and present progress on the Dutch pollen database project. The data are accessible as part of the European Pollen Database in the NEOTOMA palaeoecological database. Now 600 datasets are available, which include pollen counts from the pioneers of pollen analysis as well as recent studies in the Netherlands. We provide a brief history of pollen analysis in the Netherlands to put the origin of these datasets into perspective. The historical overview does not attempt completeness but highlights the main developments throughout the last 100 years of research.
Palliative Effects of Kaolin on Citrus Plants Under Controlled Stress Conditions of High Temperature and High Light Intensity
Authors: Terán Fátima , Vives-Peris Vicente , López-Climent María F. , Gómez-Cadenas Aurelio , Pérez-Clemente Rosa M. 
Published: September 2023

AbstractUnder field conditions high temperatures are usually associated to high light intensity for periods of time that are getting longer because of global warming caused by climate change. These adverse conditions lead to significant reductions in yield and fruit quality in crops of great economic relevance such as citrus. In this work, the effect of high temperatures and high light intensity occurring alone or simultaneously has been studied in Carrizo citrange plants, a citrus genotype widely used as a rootstock, and the mitigating effect of kaolin (KL) evaluated. The combined stress conditions affected the plants in a unique manner at both, physiological and hormonal regulation levels, different to the effects of each individual stress. KL alleviated the deleterious effect of combined stress in different ways: (i) reducing leaf damage and abscission, (ii) improving physiological and gas exchange parameters, (iii) decreasing foliar proline content, (iv) increasing chlorophyll content, (v) preventing carotenoid degradation, and (vi) modulating levels of hormones and their precursors involved in plant responses to stress [abscisic acid (ABA), salicylic acid (SA), cinnamic acid (CA), indole-3-acetic acid (IAA), jasmonic acid (JA) and 12-oxophytodienoic acid (OPDA)].
Coronary microvascular dysfunction is a hallmark of all subtypes of MINOCA
Authors: Milzi Andrea , Dettori Rosalia , Lubberich Richard Karl , Reith Sebastian , Frick Michael , Burgmaier Kathrin , Marx Nikolaus , Burgmaier Mathias 
Published: September 2023

Abstract
Introduction
Myocardial infarction without obstructive coronary artery disease (MINOCA) is a heterogeneous clinical condition presenting with myocardial necrosis not due to an obstruction of a major coronary artery. Recently, a relevant role of coronary microvascular dysfunction (CMD) in the pathogenesis of MINOCA has been suggested; however, data on this are scarce. Particularly, it is unclear if CMD is equally present in all subtypes of MINOCA or differentially identifies one or more of these conditions. Therefore, the aim of this study was to assess CMD in all three coronary vessels of MINOCA patients, relating it with the clinical subtype.

Methods
We retrospectively assessed coronary microvascular function in all three coronary territories by means of angiography-based index of microvascular resistance (aIMR) in 92 patients (64 with working diagnosis of MINOCA, 28 control patients). To further assess the association of CMD with MINOCA subtypes, MINOCA patients were subdivided according to clinical data in coronary cause (n = 13), takotsubo (n = 13), infiltrative or inflammatory cardiomyopathy (n = 9) or unclear (n = 29).

Results
Patients with working diagnosis of MINOCA showed a significantly elevated average aIMR compared to control patients (30.5 ± 7.6 vs. 22.1 ± 5.9, p < 0.001) as a marker of a relevant CMD; these data were consistent in all vessels. Among MINOCA subtypes, no significant difference in average aIMR could be detected between patients with coronary cause (33.2 ± 6.6), takotsubo cardiomyopathy (29.2 ± 6.9), infiltrative or inflammatory cardiomyopathy (28.1 ± 6.8) or unclear cause (30.6 ± 8.5; p = 0.412). Interestingly, aIMR was significantly elevated in the coronary vessel supplying the diseased myocardium compared with other vessels (31.9 ± 11.4 vs. 27.8 ± 8.2, p = 0.049).

Conclusion
Coronary microvascular dysfunction is a hallmark of all MINOCA subtypes. This study adds to the pathophysiological understanding of MINOCA and sheds light into the role of CMD in MINOCA.
Plant-based dietary indices and biomarkers of chronic low-grade inflammation: a cross-sectional analysis of adults in Ireland
Authors: Kharaty Soraeya , Harrington Janas M. , Millar Seán R. , Perry Ivan J. , Phillips Catherine M. 
Published: September 2023

Abstract
Purpose
There is increasing interest in the health benefits of plant-based diets (PBDs). Evidence reports favourable associations with inflammatory profiles and reduced cardiovascular disease risk. However, limited studies have examined relationships between PBD indices (PDIs) and inflammatory biomarkers. We explored overall PDI, healthful PDI (hPDI) and unhealthful PDI (uPDI) associations with inflammatory biomarker profiles.

Methods
This cross-sectional analysis included 1986 middle- to older-aged adults from the Mitchelstown Cohort. PDI scores were calculated using validated food frequency questionnaires. PDI score associations with inflammatory biomarkers were assessed via linear regression analysis, with adjustment for potential confounders.

Results
Comparison of quintiles (Q5 vs Q1) revealed lower concentrations of C-reactive protein (CRP), interleukin 6 (IL-6), white blood cells (WBCs), neutrophils and monocytes, and the leptin-to-adiponectin ratio (PDI and hPDI P < 0.05); lower leptin (PDI, P < 0.05), and complement component 3 (C3), tumour necrosis factor alpha (TNF-α), plasminogen activator inhibitor 1, lymphocytes and eosinophils (hPDI, P < 0.05); and higher concentrations of adiponectin (PDI and hPDI, P < 0.05). Conversely, higher concentrations of C3, CRP, IL-6, TNF-α, resistin, WBCs, neutrophils, lymphocytes, monocytes and eosinophils, and the neutrophil-to-lymphocyte ratio, and lower adiponectin concentrations were observed comparing uPDI quintiles (P < 0.05). In fully adjusted regression models, higher hPDI scores were associated with lower concentrations of C3, TNF-α, WBCs, neutrophils and monocytes (all P < 0.01). Higher uPDI scores were associated with higher C3 and TNF-α concentrations (all P < 0.01).

Conclusion
This study provides evidence that a more healthful PBD is associated with a more favourable inflammatory profile and that a more unhealthful PBD is associated with the reverse.
Factors influencing choice of treatment for proximal humeral fractures elaborated in a Delphi consensus process
Authors: Klute Lisa , Henssler Leopold , Schliemann Benedikt , Königshausen Matthias , Weber Nadine , Alt Volker , Kerschbaum Maximilian 
Published: September 2023

Abstract
Introduction
Hitherto, the decision-making process for treatment of proximal humerus fractures (PHF) remains controversial, with no established or commonly used treatment regimens. Identifying fracture- and patient-related factors that influence treatment decisions is crucial for the development of such treatment algorithms. The objective of this study was to define a Delphi consensus of clinically relevant fracture- and patient-related factors of PHF for clinical application and scientific research.

Methods
An online survey was conducted among an international panel of preselected experienced shoulder surgeons. An evidence-based list of fracture-related and patient-related factors affecting treatment outcome after PHF was generated and reviewed by the members of the committee through online surveys. The proposed factors were revised for definitions, and suggestions from the first round mentioned in the free text were included as possible factors in the second round of surveys. Consensus was defined as having at least a two-thirds majority agreement.

Results
The Delphi consensus panel consisted of 18 shoulder surgeons who completed 2 rounds of online surveys. There was an agreement of more than two-thirds of the panel for three fracture-related factors affecting treatment decision in the case of PHF: head-split fracture, dislocated tuberosities, and fracture dislocation. Of all patient-related factors, a two-thirds consensus was reached for two factors: age and rotator cuff tear arthropathy.

Conclusion
This study successfully conducted a Delphi consensus on factors influencing decision-making in the treatment of proximal humeral fractures. The documented factors will be useful for clinical evaluation and scientific validation in future studies.
Frequency and predictors of poststroke epilepsy after mechanical thrombectomy for large vessel occlusion stroke: results from a multicenter cohort study
Authors: Gruber Joachim , Gattringer Thomas , Mayr Georg , Schwarzenhofer Daniel , Kneihsl Markus , Wagner Judith , Sonnberger Michael , Deutschmann Hannes , Haidegger Melanie , Fandler-Höfler Simon , Ropele Stefan , Enzinger Christian , von Oertzen Tim 
Published: September 2023

Abstract
Background
Poststroke epilepsy (PSE) represents an important complication of stroke. Data regarding the frequency and predictors of PSE in patients with large-vessel occlusion stroke receiving mechanical thrombectomy (MT) are scarce. Furthermore, information on acute and preexisting lesion characteristics on brain MRI has not yet been systematically considered in risk prediction of PSE. This study thus aims to assess PSE risk after acute ischemic stroke treated with MT, based on clinical and MRI features.

Methods
In this multicenter study from two tertiary stroke centers, we included consecutive acute ischemic stroke patients who had received MT for acute intracranial large vessel occlusion (LVO) between 2011 and 2017, in whom post-interventional brain MRI and long term-follow-up data were available. Infarct size, affected cerebrovascular territory, hemorrhagic complications and chronic cerebrovascular disease features were assessed on MRI (blinded to clinical information). The primary outcome was the occurrence of PSE (> 7 days after stroke onset) assessed by systematic follow-up via phone interview or electronic records.

Results
Our final study cohort comprised 348 thrombectomy patients (median age: 67 years, 45% women) with a median long-term follow-up of 78 months (range 0–125). 32 patients (9%) developed PSE after a median of 477 days (range 9–2577 days). In univariable analyses, larger postinterventional infarct size, infarct location in the parietal, frontal or temporal lobes and cerebral microbleeds were associated with PSE. Multivariable Cox regression analysis confirmed larger infarct size (HR 3.49; 95% CI 1.67–7.30) and presence of cerebral microbleeds (HR 2.56; 95% CI 1.18–5.56) as independent predictors of PSE.

Conclusion
In our study, patients with large vessel occlusion stroke receiving MT had a 9% prevalence of PSE over a median follow-up period of 6.5 years. Besides larger infarct size, presence of cerebral microbleeds on brain MRI predicted PSE occurrence.
Molecular neuropathology: an essential and evolving toolbox for the diagnosis and clinical management of central nervous system tumors
Authors: Bertero Luca , Mangherini Luca , Ricci Alessia Andrea , Cassoni Paola , Sahm Felix 
Published: September 2023

AbstractMolecular profiling has transformed the diagnostic workflow of CNS tumors during the last years. The latest WHO classification of CNS tumors (5th edition), published in 2021, pushed forward the integration between histopathological features and molecular hallmarks to achieve reproducible and clinically relevant diagnoses. To address these demands, pathologists have to appropriately deal with multiple molecular assays mainly including DNA methylation profiling and DNA/RNA next generation sequencing. Tumor classification by DNA methylation profiling is now a critical tool for many diagnostic tasks in neuropathology including the assessment of complex cases, to evaluate novel tumor types and to perform tumor subgrouping in hetereogenous entities like medulloblastoma or ependymoma. DNA/RNA NGS allow the detection of multiple molecular alterations including single nucleotide variations, small insertions/deletions (InDel), and gene fusions. These molecular markers can provide key insights for diagnosis, for example, if a tumor-specific mutation is detected, but also for treatment since targeted therapies are progressively entering the clinical practice. In the present review, a brief, but comprehensive overview of these tools will be provided, discussing their technical specifications, diagnostic value, and potential limitations. Moreover, the importance of molecular profiling will be shown in a representative series of CNS neoplasms including both the most frequent tumor types and other selected entities for which molecular characterization plays a critical role.
Cardiovascular outcomes improve in children with renovascular hypertension following endovascular and surgical interventions
Authors: Redhead Emily C. G. , Paessler Alicia , Arslan Zainab , Patel Premal , Minhas Kishore , Forman Colin , Hollis Paolo , Lava Sebastiano , Ionescu Florin , Manuel Devi , Ray Samiran , Kessaris Nicos , Giardini Alessandro , Ratnamma Vineetha , Dobby Nadine , Tullus Kjell , Simmonds Jacob , Stojanovic Jelena 
Published: September 2023

Abstract
Background
Renovascular hypertension (RenoVH) is a cause of hypertension in children. A common cause of RenoVH is renal artery stenosis which acts by reducing blood supply to renal parenchyma and activating the renin–angiotensin–aldosterone axis, often leading to cardiac remodelling. This longitudinal observational study aims to describe occurrence of cardiovascular changes secondary to RenoVH and also any improvement in cardiac remodelling after successful endovascular and/or surgical intervention.

Methods
All patients with RenoVH referred to our centre, who received ≥ 1 endovascular intervention (some had also undergone surgical interventions) were included. Data were collected by retrospective database review over a 22-year period. We assessed oscillometric blood pressure and eight echocardiographic parameters pre- and post-intervention.

Results
One hundred fifty-two patients met inclusion criteria and had on average two endovascular interventions; of these children, six presented in heart failure. Blood pressure (BP) control was achieved by 54.4% of patients post-intervention. Average z-scores improved in interventricular septal thickness in diastole (IVSD), posterior Wall thickness in diastole (PWD) and fractional shortening (FS); left ventricular mass index (LVMI) and relative wall thickness (RWT) also improved. PWD saw the greatest reduction in mean difference in children with abnormal (z-score reduction 0.25, p < 0.001) and severely abnormal (z-score reduction 0.23, p < 0.001) z-scores between pre- and post-intervention echocardiograms. Almost half (45.9%) had reduction in prescribed antihypertensive medications, and 21.3% could discontinue all antihypertensive therapy.

Conclusions
Our study reports improvement in cardiac outcomes after endovascular + / − surgical interventions. This is evidenced by BP control, and echocardiogram changes in which almost half achieved normalisation in systolic BP readings and reduction in the number of children with abnormal echocardiographic parameters.

Graphical abstract
Kidney transplantation in low- and middle-income countries: the Transplant Links experience
Authors: Roberts Lydia E. , Kaur Amrit , Jewitt-Harris Jennie , Ready Andrew , Milford David V. 
Published: September 2023

AbstractPaediatric kidney failure is a global problem responsible for significant childhood morbidity and mortality. The gold-standard treatment is kidney transplantation. However, the availability of kidney transplantation remains limited in some low- and middle-income countries (LMICs). Transplant Links Community (TLC) is a UK-based charity that mentors units in LMICs wishing to start kidney transplantation; the ultimate goal is for these units to become self-sufficient. TLC provides this support through in-person training visits and skill transfer, plus direct mentorship from the UK that is maintained over many years. From such mentoring programmes, it is evident that there are numerous challenges in the initial establishment and long-term maintenance of kidney transplant services, with specific and unique barriers applying to setting up paediatric transplant programmes compared to their adult counterparts. This review summarises TLC’s first-hand experience of developing paediatric kidney transplantation services in LMICs over the past 15 years, the challenges encountered, and the major ongoing barriers that must be addressed to facilitate further progress in delivering transplantation services to children globally.
How does pollen production of allergenic species differ between urban and rural environments?
Authors: Jetschni Johanna , Fritsch Markus , Jochner-Oette Susanne 
Published: September 2023

AbstractPollen production is one plant characteristic that is considered to be altered by changes in environmental conditions. In this study, we investigated pollen production of the three anemophilous species Betula pendula, Plantago lanceolata, and Dactylis glomerata along an urbanization gradient in Ingolstadt, Germany. We compared pollen production with the potential influencing factors urbanization, air temperature, and the air pollutants nitrogen dioxide (NO2) and ozone (O3). While we measured air temperature in the field, we computed concentration levels of NO2 and O3 from a land use regression model. The results showed that average pollen production (in million pollen grains) was 1.2 ± 1.0 per catkin of Betula pendula, 5.0 ± 2.4 per inflorescence of Plantago lanceolata, and 0.7 ± 0.5 per spikelet of Dactylis glomerata. Pollen production was higher in rural compared to urban locations on average for B. pendula (+ 73%) and P. lanceolata (+ 31%), while the opposite was the case for D. glomerata (− 14%). We found that there was substantial heterogeneity across the three species with respect to the association of pollen production and environmental influences. Pollen production decreased for all species with increasing temperature and urbanization, while for increasing pollutant concentrations, decreases were observed for B. pendula, P. lanceolata, and increases for D. glomerata. Additionally, pollen production was found to be highly variable across species and within species—even at small spatial distances. Experiments should be conducted to further explore plant responses to altering environmental conditions.
An improved rough set strategy-based sine cosine algorithm for engineering optimization problems
Authors: Rizk-Allah Rizk M. , Elsodany E. 
Published: September 2023

AbstractIn this paper, a hybrid algorithm called rough sine cosine algorithm (RSCA) is introduced for solving engineering optimization problems by merging the sine cosine algorithm (SCA) with the rough set theory concepts (RST). RSCA combines the benefits of SCA and RST to focus the search for a promising region where the global solution can be found. Due to imprecise information on the optimization problems, efficient algorithms roughly identify the optimal solution for this type of uncertain data. The fundamental motive for adding the RST is to deal with the imprecision and roughness of the available information regarding the global optimal, especially for large dimensional problems. The cut concept of RST targeted the more interesting search region so the optimal operation could be sped up, and the global optimum could be reached at a low computational cost. The proposed RSCA algorithm is tested on 23 benchmark functions and 3 design problems. RSCA’s obtained results are mainly compared to the SCA, which is used as a first level of the proposed algorithm in this work and those of other algorithms in the literature. According to the comparisons, the RSCA can provide very competitive performance with different algorithms.
Prevalence and consequences of spinal pain among people with type 1 and type 2 diabetes mellitus in Denmark
Authors: Liaghat Behnam , Folkestad Lars , Skou Søren T. , Koes Bart , Stammerjohan Amalie Frost , Hartvigsen Jan 
Published: September 2023

Abstract
Purpose
To describe 1-week and 1-year prevalence of spinal pain and its consequences in relation to leisure activity, work-life, and care-seeking in people with type 1 and 2 diabetes mellitus (DM).

Methods
A cross-sectional survey including adults diagnosed with DM from two Danish secondary care centres. Using the Standardised Nordic Questionnaire, spinal pain prevalence (cervical, thoracic, lumbar) and its consequences were evaluated (proportions, 95% confidence intervals) and compared to the general population.

Results
Among 3767 people, 1-week and 1-year spinal pain prevalence were 11.6–32.4 and 18.5–49.6%, respectively, highest for lumbar pain (24.6–49.6%). The prevalence was similar between DM types for cervical and thoracic pain, but higher in type 2 for lumbar spine. Women had higher pain prevalence across spinal regions and DM types, while cervical and thoracic pain estimates were higher for age < 60 vs. ≥ 60. Within the past year, > 50% reported pain > 30 days, high proportions had reduced their activities (leisure time, 43.7–63.9%; work, 20.7–33.3%), 13.3–28.1% reported sick-leave > 30 days, and 44.3–48.5% had sought care due to spinal pain.

Conclusion
Spinal pain is common in people with type 1 and 2 DM, resulting in considerable consequences for work/leisure activities, sick-leave, and healthcare utilisation as compared to the general population.
Prospective randomized controlled trial: early weight bearing after conservative treatment of Weber B ankle fractures (pancake trial)
Authors: Stassen R. C. , Franssen S. , Meesters B. , Boonen B. , de Loos E. R. , van Vugt R. 
Published: September 2023

Abstract
Purpose
Different studies have shown that weightbearing is safe in stable transsyndesmotic, isolated lateral simple ankle fractures. Despite this evidence, AO guidelines still recommend immobilization with above-the-knee cast for 4–6 weeks for these fractures. The objective of this study was to compare the outcomes of mobilization and weightbearing to those of immobilization and non-weightbearing in patients with stable transsyndesmotic, lateral isolated simple ankle fractures.

Methods
Fifty patients were randomly assigned to permissive weightbearing in a walking boot or non-weightbearing immobilization using a below-the-knee cast. Primary outcome was ankle functionality as scored by the Olerud-Molander Ankle Score (OMAS). Secondary outcomes were radiological displacement of fracture, range of motion (ROM), calf circumference, and RAND 36-item health survey. Patients were in follow-up for 24 months.

Results
Ankle functionality after six and twelve weeks was significantly higher for the intervention group, with respectively 30 points (p = 0.001) and 10 points (p = 0.015) of difference. ROM improved significantly in the intervention group after six weeks. All fractures showed radiological progression of fracture healing. RAND 36-item showed differences in both physical (60.3 vs. 46.3, p = 0.017) and mental (78.5 vs. 58.2, p = 0.034) components in favor of the intervention group. In 16% of patients who initially showed stable fractures on radiographic imaging, joint dislocation was identified on weightbearing radiographs prior to randomization, leading to exclusion.

Conclusion
Weightbearing and mobilization using a walking boot may be a safe treatment for patients with stable Weber B fractures.