bluuebunny/crossref_metadata_2025_split huggingface dataset:
Efficacy and safety of CT-guided 125I seed implantation by coplanar template as a salvage therapy for vertebral metastases after failure of external beam radiation therapy: a retrospective study
Authors: Li Peishun , Bai Yunling , Yuan Qianqian , Man Qirong , Xing Chao , Ren Yanchen , Zhang Kaixian 
Published: April 2023

PurposeTo evaluate the efficacy safety of computed tomography (CT)-guided 125I seed implantation by coplanar template for vertebral metastases after failure of external beam radiation therapy (EBRT).Material and methodsRetrospective analysis of the clinical outcomes of 58 patients with vertebral metastases after failure of EBRT, who underwent 125I seed implantation as a salvage treatment with a CT-guided coplanar template-assisted technique from January 2015 to January 2017.ResultsThe mean post-operative NRS score decreased significantly at T4w (3.5 ± 0.9, p&lt;0.01), T8w (2.1 ± 0.9, p&lt;0.01), T12w (1.5 ± 0.7, p&lt; 0.01) and T6m (1.2 ± 0.6, p&lt; 0.01) respectively. The local control rates after 3, 6, 9 and 12 months were 100% (58/58), 93.1% (54/58), 87.9% (51/58), and 81% (47/58), respectively. The median overall survival time was 18.52months (95% CI, 16.24-20.8), and 1- and 2-year survival rates were 81% (47/58) and 34.5% (20/58), respectively. By performing a paired t-test analysis, there was no significant difference in D90, V90, D100, V100, V150, V200, GTV volume, CI, EI and HI between preoperative and postoperative (p&gt;0.05).Conclusions125I seed implantation can be used as a salvage treatment for patients with vertebral metastases after failure of EBRT.
Ion channel gene GJB2 influences the intercellular communication by Up-regulating the SPP1 signaling pathway identified by the single-cell RNA sequencing in lung adenocarcinoma
Authors: Liu Zuo , Xiao Zengtuan , Wang Xiaofei , Zhang Lianmin , Zhang Zhenfa 
Published: April 2023

ObjectiveFirstly, observe the prognostic significance and the biological functional effects of gap junction protein beta 2 (GJB2 or Cx26) in lung adenocarcinoma (LUAD). Subsequently, explore the role played by GJB2 in intercellular communication by single-cell RNA sequencing.MethodWe made a differential analysis of GJB2 expression through public databases and investigated the clinical characteristics and prognostic significance. ESTIMATE analysis and Tumor Immune Estimation Resource (TIMER) database were utilized to illustrate the association of GJB2 with immune infiltration and components of the tumor microenvironment. Gene Ontology (GO), Kyoto encyclopedia of genes and genomes (KEGG), and Gene set enrichment analysis (GSEA) were performed to study the biological function of GJB2. Cell-cell communication was analyzed using the CellChat R package through sc-RNA data.ResultsGJB2 has an outstanding prognosis value in LUAD and a close relationship was found between GJB2 and immune infiltration in LUAD. GJB2 could participate in several tumor biological processes, including extracellular matrix remodeling and upregulation of multiple cancer-related active pathways. GJB2 related hub-genes influence intercellular communication through the SPP1 signaling pathway.ConclusionOur study illustrates one mechanism by which GJB2 exerts its cancer-specific relevant effects, that is, causing changes in intercellular communication through the SPP1 signaling pathway. Blockade of this pathway may limit the functional role of GJB2 and provide us with promising new perceptions for LUAD treatment.
Formulation and validation of a baseline prognostic score for osteosarcoma treated uniformly with a non-high dose methotrexate-based protocol from a low middle income healthcare setting: a single centre analysis of 594 patients
Authors: Ganguly Shuvadeep , Sasi Archana , Khan Shah Alam , Kumar Venkatesan Sampath , Kapoor Love , Sharma Mehar Chand , Mridha Asit , Barwad Adarsh , Thulkar Sanjay , Pushpam Deepam , Bakhshi Sameer 
Published: April 2023

IntroductionThe outcomes of osteosarcoma in low middle income countries (LMICs) are different due to patients presenting in advanced stages, resource constraints and the use of non-high-dose-methotrexate (HDMTX)-based regimens. This study derived and validated a prognostic score for osteosarcoma that integrates biologic and social factors and is tailored for patients from an LMIC setting using a non-HDMTX-based protocol.Materials and methodsA retrospective study including osteosarcoma patients enrolled for treatment at a single tertiary care centre in India between 2003-19 was conducted. Baseline biologic and social characteristics were extracted from medical records and survival outcomes were noted. The cohort was randomised into a derivation and validation cohort. Multivariable Cox regression was used to identify baseline characteristics that were independently prognostic for survival outcomes in the derivation cohort. A score was derived from the prognostic factors identified in the derivation cohort and further validated in the validation cohort with estimation of its predictive ability.Results594 patients with osteosarcoma were eligible for inclusion in the study. Around one-third of the cohort had metastatic disease with 59% of the patients residing in rural areas. The presence of metastases at baseline (HR 3.39; p&lt;0.001; score=3), elevated serum alkaline phosphatase (SAP) &gt;450 IU/L (HR 1.57; p=0.001; score=1) and baseline tumour size &gt; 10 cm (HR 1.68; p&lt;0.001; score=1) were identified to be independent factors predicting inferior event free survival (EFS) and were included in development of the prognostic score. Patients were categorized as low risk (score 0), intermediate risk (score 1-3) and high risk (4-5). Harrell’s c-indices for the score were 0.682, 0.608 and 0.657 respectively for EFS in the derivation, validation and whole cohort respectively. The timed AUC of ROC was 0.67 for predicting 18-month EFS in the derivation, validation and whole cohorts while that for 36-month EFS were 0.68, 0.66 and 0.68 respectively.ConclusionsThe study describes the outcomes among osteosarcoma patients from an LMIC treated uniformly with a non-HDMTX-based protocol. Tumor size, baseline metastases and SAP were prognostic factors used to derive a score with good predictive value for survival outcomes. Social factors did not emerge as determinants of survival.
Surface electromyographic characteristics of forearm muscles after ulnar and radius fracture inchildren
Authors: Qiu Hailing , Li Fanling , Zhang Siqi , Xiao Sheng , Liu Haobo , Chen Shuangxi , Li Xin , Fang Ke , Wen Jie , Li Tingzhi 
Published: April 2023

ObjectiveTo evaluate the characteristics of forearm muscle activity in children with ulnar and radius fractures during different follow-up periods by surface electromyography.MethodsA retrospective analysis was performed on 20 children with ulnar and radius fractures treated with an elastic intramedullary nail from October 2020 to December 2021. All children were treated with transcubital casts after surgery. At 2 months and before taking out the elastic intramedullary nail, surface electromyographic signals were collected on the flexor/extension of the wrist and the maximum arbitrary isometric contraction of the grip strength in the forearm flexor and extensor muscles of the forearm. The root-mean-square values and integrated EMG values of the superficial flexor and extensor digitalis of the healthy side and the affected side were collected at the last follow-up and 2 months after surgery, and the co-systolic ratio was calculated. The root-mean-square values and co-systolic ratio were compared and analyzed, and the Mayo wrist function score was evaluated.ResultsThe mean follow-up time was (8.4 ± 2.85) months. Mayo scores were (87.42 ± 13.01) and (97.69 ± 4.50) points at the last follow-up and two months after surgery, respectively (p &lt; 0.05). In the test of grip strength, 2 months after surgery, the grip strength of the affected side was lower than that of the healthy side (p &lt; 0.05), and the maximum and mean values of the superficial flexor of the affected side were lower than those of the healthy side (p &lt; 0.05). At the last follow-up, there was no difference in the grip strength between the affected side and the healthy side (p &gt; 0.05), and no difference in the maximum RMS, mean RMS and cooperative contraction ratio of the superficial flexor and digital extensor muscles between the affected side and the healthy side (p &gt; 0.05).ConclusionSatisfactory results can be obtained after elastic intramedullary napping in children with ulnar and radius fractures. However, 2 months after surgery, the grip strength of the affected side is small, and the electrical activity of the forearm muscle is low during flexion and extension activities of the wrist joint, which has not returned to normal, suggesting that children orthopaedic clinicians should remind children to conduct timely and effective rehabilitation training after the removal of the cast.
Population pharmacokinetic analysis and dosing regimen optimization of teicoplanin in critically ill patients with sepsis
Authors: Chen Chao‐Yang , Xie Min , Gong Jun , Yu Ning , Wei Ran , Lei Li‐Li , Zhao Si‐Miao , Li Ruo‐Ming , Dong Xiu , Zhang Xiang‐Lin , Zhou Ying , Li Shuang‐Ling , Cui Yi‐Min 
Published: April 2023

Objectives: Teicoplanin has been extensively used in the treatment for infections caused by gram-positive bacteria including methicillin-resistant Staphylococcus aureus (MRSA). However, current teicoplanin treatment is challenging due to relatively low and variable concentrations under standard dosage regimens. This study aimed to investigate the population pharmacokinetics (PPK) characteristics of teicoplanin in adult sepsis patients and provide recommendations for optimal teicoplanin dosing regimens.Methods: A total of 249 serum concentration samples from 59 septic patients were prospectively collected in the intensive care unit (ICU). Teicoplanin concentrations were detected, and patients’ clinical data were recorded. PPK analysis was performed using a non-linear, mixed-effect modeling approach. Monte Carlo simulations were performed to evaluate currently recommended dosing and other dosage regimens. The optimal dosing regimens were defined and compared by different pharmacokinetic/pharmacodynamic parameters, including trough concentration (Cmin), the ratio of 24-h area under the concentration-time curve to the minimum inhibitory concentration (AUC0-24/MIC), as well as the probability of target attainment (PTA) and the cumulative fraction of response (CFR) against MRSA.Results: A two-compartment model adequately described the data. The final model parameter estimates for clearance, central compartment volume of distribution, intercompartmental clearance and peripheral compartment volume were 1.03 L/h, 20.1 L, 3.12 L/h and 101 L, respectively. Glomerular filtration rate (GFR) was the only covariate that significantly affected teicoplanin clearance. Model-based simulations revealed that 3 or 5 loading doses of 12/15 mg/kg every 12 h followed by a maintenance dose of 12/15 mg/kg every 24 h–72 h for patients with different renal functions were required to achieve a target Cmin of 15 mg/L and a target AUC0-24/MIC of 610. For MRSA infections, PTAs and CFRs were not satisfactory for simulated regimens. Prolonging the dosing interval may be easier to achieve the target AUC0-24/MIC than reducing the unit dose for renal insufficient patients.Conclusion: A PPK model for teicoplanin in adult septic patients was successfully developed. Model-based simulations revealed that current standard doses may result in undertherapeutic Cmin and AUC, and a single dose of at least 12 mg/kg may be needed. AUC0-24/MIC should be preferred as the PK/PD indicator of teicoplanin, if AUC estimation is unavailable, in addition to routine detection of teicoplanin Cmin on Day 4, follow-up therapeutic drug monitoring at steady-state is recommended.
Binary classification of non-specific low back pain condition based on the combination of B-mode ultrasound and shear wave elastography at multiple sites
Authors: Yu Xiaocheng , Xu Xiaohua , Huang Qinghua , Zhu Guowen , Xu Faying , Liu Zhenhua , Su Lin , Zheng Haiping , Zhou Chen , Chen Qiuming , Gao Fen , Lin Mengting , Yang Shuai , Chiang Mou-Hsun , Zhou Yongjin 
Published: April 2023

Introduction: Low back pain (LBP) is a prevalent and complex condition that poses significant medical, social, and economic burdens worldwide. The accurate and timely assessment and diagnosis of LBP, particularly non-specific LBP (NSLBP), are crucial to developing effective interventions and treatments for LBP patients. In this study, we aimed to investigate the potential of combining B-mode ultrasound image features with shear wave elastography (SWE) features to improve the classification of NSLBP patients.Methods: We recruited 52 subjects with NSLBP from the University of Hong Kong-Shenzhen Hospital and collected B-mode ultrasound images and SWE data from multiple sites. The Visual Analogue Scale (VAS) was used as the ground truth to classify NSLBP patients. We extracted and selected features from the data and employed a support vector machine (SVM) model to classify NSLBP patients. The performance of the SVM model was evaluated using five-fold cross-validation and the accuracy, precision, and sensitivity were calculated.Results: We obtained an optimal feature set of 48 features, among which the SWE elasticity feature had the most significant contribution to the classification task. The SVM model achieved an accuracy, precision, and sensitivity of 0.85, 0.89, and 0.86, respectively, which were higher than the previously reported values of MRI.Discussion: In this study, we aimed to investigate the potential of combining B-mode ultrasound image features with shear wave elastography (SWE) features to improve the classification of non-specific low back pain (NSLBP) patients. Our results showed that combining B-mode ultrasound image features with SWE features and employing an SVM model can improve the automatic classification of NSLBP patients. Our findings also suggest that the SWE elasticity feature is a crucial factor in classifying NSLBP patients, and the proposed method can identify the important site and position of the muscle in the NSLBP classification task.
Experimental investigation of training schedule on home-based working memory training in healthy older adults
Authors: Booth Samantha J. , Brown Laura J. E. , Taylor Jason R. , Pobric Gorana 
Published: April 2023

IntroductionThe efficacy of working memory training (WMT) for cognitive enhancement in healthy older adults has been extensively investigated. Typically, WMT results in improved performance on the training task, but limited or no transfer of improvement to other cognitive tasks. Accordingly, there is a need to identify optimal intervention parameters to maximize training and transfer task effects of WMT. The current study aimed to investigate the effect of training schedule on training and transfer task performance of WMT in healthy older adults. A secondary aim was to examine the feasibility of participants performing the intervention online at home, unsupervised, and using their personal devices.MethodsParticipants (N = 71; mean age: 66 years) completed sixteen WMT or active-control sessions over eight (distributed) or four (intensive) weeks. Adaptive verbal and spatial n-back tasks were used as the WMT tasks. We tested near transfer effects to a digit-span task and far transfer effects to an abstract relational reasoning task.ResultsParticipants successfully performed the cognitively demanding intervention using their own devices, online at home, and with minimal contact with the researcher. We observed a significant improvement in WMT task performance in the WMT group relative to active-controls, but no evidence of near or far transfer. Similar training effects were observed irrespective of the intensity of the training schedule.DiscussionOur results suggest that comparable benefits could be observed when using less intensive schedules that may be more easily accommodated into everyday life.
Sensor-Generated Data for Evaluation of Subclinical Mastitis Treatment Effectiveness with Garlic Extract (Allicin) in Dairy Cattle
Authors: Antanaitis Ramūnas , Anskienė Lina , Džermeikaitė Karina , Bačėninaitė Dovilė , Januškauskas Aloyzas , Sincevičius Kęstutis , Baumgartner Walter , Klein Anton 
Published: April 2023

The aim of this study was to determine the impact of subclinical mastitis treatment in dairy cattle on biomarkers registered with in-line sensors such as milk yield (MY), electric milk conductivity (EC), rumination time (RT), and somatic cell count (SCC). At the start of the experiment, all cows according to SCC level were divided into two groups: healthy cows (n = 30, with SCCs less than 200,000 per mL and without the growth of bacteria in the milk samples) and cows with subclinical mastitis (n = 32), with SCC levels greater than 200,000 per mL and with growth of bacteria. Streptococcus spp. was found in 15 samples, and Strep. uberis was found in 17 samples. Streptococcus spp. and Strep. uberis were sensitive to amoxicillin and calvulanic acid. According to these results, 32 cows with subclinical mastitis were treated with two treatment protocols: one 1 (n = 16) and two (n = 16). In the first protocol, we used SCC boluses and nonsteroidal anti-inflammatory drugs (SCCB and NSAID). The second protocol consists of intramammary antibiotics and anti-inflammatory medications (Synulox LC and NSAIDs). All parameters (MY, EC, RT, and SCC) were recorded with Lely Astronaut® A3 milking robots on the day of mastitis diagnosis (0 day) and 14 days after treatment began. All animal experimental procedures were approved by the ethical committee; the approval number is PK01696. On the basis of our findings, we may infer that SCC boluses and NSAIDs are effective in treating subclinical mastitis. After 14 days of treatment, the electrical conductivity of milk in cows treated with AB and NSAID was also higher in all quarters of the udder compared to cows treated with SCCB + NSAID. The RT of cows on disease diagnosis day of cows treated with AB and NSAID was 11.41% lower compared to cows treated with SCCB and NSAID, while the RT of cows after 14 days treated with AB and NSAID was 7.01% lower compared to cows treated with SCCB and NSAID. On the practical side, for treatment of subclinical mastitis, we recommend using a feed supplement SCC bolus (one per os) with a composition containing Meloxicam 20 mg with a single subcutaneous injection at a dosage of 2.5 mL per 100 kg body weight.
Acute and long-term pathological effects of COVID-19 on the placenta during second trimester and labor.
Authors: Taş Fatih , Erdemci̇ Fikri , Yilmaz Mehmet , Aşir Fırat , Özüdoğru Osman , Deveci̇ Engin 
Published: April 2023

Background: COVID-19 pandemic has affected all the world. The consequence of the COVID-19 infection causes many disorders in many organs, one of them is the placenta. COVID-19 disease has long-term effects on various tissues after recovery. The aim of this study was to investigate the placentas of pregnant women with healthy, COVID-19 positive during the second trimester and labor.
Material and methods: A total of twenty-four pregnant women (8 patients per each group) were included in the study. Their placentas were processed for routine paraffin wax embedding protocol. The blood parameters of patients were recorded. Placental tissues were stained with hematoxylin-eosin dye and immune stained with TNF-α and ADAMTS-8. Statistical analysis was performed on blood and histological parameters.
Results: AST and CRP values of biochemical parameters were higher in women with second trimester and labor groups than in the healthy group. Also, a significant increase in ALT values was observed in the labor group. Normal histology was observed in the placentas of healthy patients. More histopathology was recorded in the placentas of COVID-19 infected women compared to healthy placentas. The expression of TNF-α and ADAMTS-8 were found significantly higher in the COVID-19 placentas compared to the non-COVID-19 group.
Conclusions: COVID-19 infection can cause pathological changes during pregnancy and labor. This study shows that COVID-19 not only acutely has adverse effects on placental pathologies but also has long-term effects. TNF-α and ADAMTS-8 primary antibodies can be a guide in demonstrating these effects.
Strategi Pemasaran Pony Plop PT. XYZ pada Periode 2022-2024 di Jabodetabek
Authors: Citra Sonia Fergina , Nadila Karina , Kurnia Pepey Riawati , Puspita Anggun Pesona Intan 
Published: April 2023

Nowadays, Many Indonesians are more aware of living a healthy lifestyle. Thus, such conditions opened many opportunities in the food industry, starting with the emergence of healthy catering companies, healthy snacks, and now healthy snacks made especially for toddlers. Providing snacks for toddlers is very important in their development days as their hunger booster and nutritional support supplement. PT. XYZ was founded in 2018 to sell healthy toddler snacks made from fresh ingredients from nearby farmers. Many of their products have become parent’s and toddler’s favorites, yet their Pony Plop product, launched in 2020, has decreased sales despite many awards won. Pony Plop is similar to the meringue (a dessert made from whipped egg white), which is quite unfamiliar to many Indonesians, although it has considerable nutritional value. Their marketing strategy for Pony Plop has not yet gained sufficient awareness, resulting in poor sales. By conducting an internal analysis, SWOT, and portfolio analysis, suitable marketing strategies were chosen for PT. XYZ. Those strategies are: Improve customer loyalty and retention, Build marketing advantage, and Improve differentiation advantage. Implementing these strategies increased awareness and sales of PT.XYZ is to be expected.
NATIONAL MINORITIES IN THE EMIGRATION MOVEMENT FROM POLAND TO LATVIA (1928-1939)
Authors: Grzybowski J. 
Published: April 2023

The aim of the article is to comprehensively show the participation of representatives of national minorities among Polish economic migrants in Latvia in the years 1928 - 1939.
Methodology. When writing the article, the basic principles of historical knowledge were used: historicism, scientificity, objectivity. Specific search tasks of the research were solved by problem-chronological means (optimal involvement of the thematic literature and source base); historical-comparative (analysis of various sources and formation of relevant research provisions and conclusions); critical analysis (critical attitude to various sources) methods.
The scientific noveltyconsists of a given publication is the introduction into circulation of a significant amount of hitherto unknown scientific sources concerning the nationality structure of Polish economic migrants in Latvia in the discussed period.
Conclusions. The economic emigration from Poland to Latvia was a consequence of the socio-economic situation in both countries. The internal determinants of emigration were agrarian overpopulation and poverty in the Polish countryside. In this situation, many people saw a chance to improve their lives abroad. The problem of the excess of hands to work in the Polish countryside coincided with a period of economic growth in Latvia. At a time when Poland was struggling with overpopulation and an excess of labor in the countryside, the Baltic country faced scarcity of hands in agriculture. The emigration movement to Latvia lasted from 1928 to 1939. The number of Polish workers employed in Latvian agriculture is estimated at over 100,000 people. Polish emigrants were characterized by great ethnic diversity. Poles, Belarusians and Lithuanians predominated in numbers, who during their stays abroad became the object of interest of representatives of the Lithuanian and Belarusian diaspora in Latvia.