bluuebunny/crossref_metadata_2025_split huggingface dataset:
Characterization of the bioaccumulation and toxicity of copper pyrithione, an antifouling compound, on juveniles of rainbow trout
Authors: Bourdon Charlotte , Cachot Jérôme , Gonzalez Patrice , Couture Patrice 
Published: February 2023

AbstractSince the global ban on tributyltin in antifouling paints in 2008 by the International Maritime Organization, new products have been developed and brought to the market. Among them, copper pyrithione (CuPT) is used, but its mechanisms of toxicity remain little known. This project aimed to identify and measure the impacts of aqueous exposure to CuPT, an organic compound, and compare it to ionic Cu2+added in the form of its inorganic salt CuSO4, in equivalent Cu2+molar concentrations, on rainbow trout (Oncorhynchus mykiss) juveniles under controlled laboratory conditions. A 24-hour acute exposure was performed with nominal concentrations of 50 and 100 µg/L Cu from either CuSO4or CuPT (labelled CuSO4_50, CuSO4_100, CuPT_50 and CuPT_100, respectively). The CuPT_100 condition induced 85 % mortality in 15 hours and the CuPT_50 condition induced 5 % mortality in the same period. A chronic exposure was then performed with nominal concentrations of 1 and 10 µg/L Cu from CuPT and 10 µg/L Cu2+from CuSO4(labelled CuSO4_1, CuSO4_10, CuPT_1 and CuPT_10, respectively). Measured aqueous concentrations of Cu2+were slightly higher than nominal concentrations for the lower concentrations, but lower for the CuPT_10 condition. The 8- and 16-day toxicokinetics showed a greater accumulation of copper in the gills of fish exposed to CuPT compared to fish exposed to Cu2+from CuSO4. The CuPT_10 condition induced 35 and 38 % mortality after 8 and 16 days of exposure, while no mortality was observed in the CuSO4_10 condition. The growth of juveniles was not impacted during the 16 days of exposure for any condition. The activity of antioxidant enzymes (CAT, SOD, GPx) did not respond to exposure to either contaminant. The expression of genes involved in the antioxidant response (sod1,sod2,gpx), detoxification (cyp1a,mt1x,mt2x), Cu transport (ctr1,ctr2,slc11a2), energy metabolism (AcoAc,cox, 12S) and cell cycle regulation (bax) strongly decreased at Day 8 in the gills and at Day 16 in the liver of CuPT-exposed fish in comparison to controls at the same time point. This study clearly showed that the toxicity of Cu in the form of CuPT was much higher than that of ionic Cu from CuSO4and provides new information on the compound that will be useful to develop regulations concerning its use and release in the aquatic environment.Credit author statementCharlotte Bourdon: Methodology, Validation, Investigation, Writing – Original Draft. Jérôme Cachot: Conceptualization, Methodology, Validation, Investigation, Writing – Original Draft, Supervision. Patrice Gonzalez: Validation, Investigation, Writing – Original Draft, Supervision, Funding acquisition. Patrice Couture: Conceptualization, Methodology, Validation, Investigation, Writing – Original Draft, Supervision, Project administration, Funding acquisition.
Functional Trajectories during innate spinal cord repair
Authors: Jensen Nicholas O. , Burris Brooke , Zhou Lili , Yamada Hunter , Reyes Catrina , Mokalled Mayssa H. 
Published: February 2023

AbstractAdult zebrafish are capable of anatomical and functional recovery following severe spinal cord injury. Axon growth, glial bridging and adult neurogenesis are hallmarks of cellular regeneration during spinal cord repair. However, the correlation between these cellular regenerative processes and functional recovery remains to be elucidated. Whereas the majority of established functional regeneration metrics measure swim capacity, we hypothesize that gait quality is more directly related to neurological health. Here, we performed a longitudinal swim tracking study for sixty individual zebrafish spanning eight weeks of spinal cord regeneration. Multiple swim parameters as well as axonal and glial bridging were integrated. We established rostral compensation as a new gait quality metric that highly correlates with functional recovery. Tensor component analysis of longitudinal data supports a correspondence between functional recovery trajectories and neurological outcomes. Moreover, our studies predicted and validated that a subset of functional regeneration parameters measured 1 to 2 weeks post-injury is sufficient to predict the regenerative outcomes of individual animals at 8 weeks post-injury. Our findings established new functional regeneration parameters and generated a comprehensive correlative database between various functional and cellular regeneration outputs.
Insects pollinated flowering plants for most of angiosperm evolutionary history
Authors: Stephens Ruby E. , Gallagher Rachael V. , Dun Lily , Cornwell Will , Sauquet Hervé 
Published: February 2023

SummaryPollination is a fundamental process driving the speciation of angiosperms (flowering plants). Most contemporary angiosperms are insect pollinated, but abiotic pollination by wind or water and vertebrate pollination by birds or mammals occurs in many lineages. We model the evolution of pollination across angiosperms and quantify the timing and environmental associations of pollination shifts.We use a robust dated phylogeny and trait-independent species-level sampling across all families of angiosperms to model the evolution of pollination modes. Data on the pollination system or syndrome of 1160 species were collated from primary literature.Angiosperms were ancestrally insect pollinated, and insects have pollinated angiosperms for approximately 86% of angiosperm evolutionary history. Wind pollination evolved at least 42 times, with few reversals back to animal pollination. Transitions between insect and vertebrate pollination were more frequent: vertebrate pollination evolved at least 39 times from an insect pollinated ancestor with at least 26 reversals. The probability of wind pollination increases with habitat openness (measured by Leaf Area Index) and with distance from the equator.Our reconstruction of pollination across angiosperms sheds light on a key question in angiosperm macroevolution, highlighting the long history of interactions between insect pollinators and angiosperms still vital to global biodiversity today.
Two teosintes made modern maize
Authors: Yang Ning , Wang Yuebin , Liu Xiangguo , Jin Minliang , Vallebueno-Estrada Miguel , Calfee Erin , Chen Lu , Dilkes Brian P. , Gui Songtao , Fan Xingming , Harper Thomas K. , Kennett Douglas J. , Li Wenqiang , Lu Yanli , Luo Jingyun , Mambakkam Sowmya , Menon Mitra , Snodgrass Samantha , Veller Carl , Wu Shenshen , Wu Siying , Xiao Yingjie , Yang Xiaohong , Stitzer Michelle C. , Runcie Daniel , Yan Jianbing , Ross-Ibarra Jeffrey 
Published: February 2023

AbstractDespite its global importance as a crop with broad economic, dietary, and cultural importance, the origins of maize and its closest wild relatives remained the topic of vigorous debate for nearly a century. Molecular analyses ultimately concluded that maize was domesticated once from a common ancestor with its closest extant relative, the lowland wild grassZea maysssp.parviglumis. But neither the current genetic model nor earlier models based on archaeological data account for the totality of available data, and recent work has highlighted the potential contribution of a second wild relative, the highlandZea maysssp.mexicana. Here we present a detailed population genetic analysis of the contributions of both wild taxa to modern maize diversity using the largest sample of traditional maize varieties sequenced to date. We show that all modern maize can trace its origin to an ancient admixture event between domesticated ancient maize andZea maysssp.mexicanain the highlands of Mexico ca 5300 cal BP, some 4,000 years after domestication began. We show that variation in admixture is a key component of modern maize genetic and phenotypic diversity, both at the level of individual loci and as a factor driving a substantial component of additive genetic variation across a number of agronomic traits. Our results clarify the long-debated origin of modern maize, highlight the potential contributions of crop wild relatives to agronomic improvement, and raise new questions about the anthropogenic mechanisms underlying multiple waves of dispersal throughout the Americas.One-Sentence SummaryOur results clarify the long-debated origin of modern maize and highlight the contributions of crop wild relatives to the agronomic improvement of modern varieties.
Live imaging of excitable axonal microdomains in ankyrin-G-GFP mice
Authors: Thome Christian , Janssen Jan Maximilian , Karabulut Seda , Acuna Claudio , D’Este Elisa , Soyka Stella J. , Baum Konrad , Bock Michael , Lehmann Nadja , Roos Johannes , Stevens Nikolas A. , Hasegawa Masashi , Ganea Dan A. , Benoit Chloé M. , Gründemann Jan , Min Lia , Bird Kalynn M. , Schultz Christian , Bennett Vann , Jenkins Paul M. , Engelhardt Maren 
Published: February 2023

AbstractThe axon initial segment (AIS) constitutes not only the site of action potential initiation, but also a hub for activity-dependent modulation of output generation. Recent studies shedding light on AIS function used predominantly post-hoc approaches since no robust murinein vivolive reporters exist. Here, we introduce a reporter line in which the AIS is intrinsically labeled by an ankyrin-G-GFP fusion protein activated by Cre recombinase, tagging the nativeAnk3gene. Using confocal, superresolution, and two-photon microscopy as well as whole-cell patch-clamp recordingsin vitro, ex vivo, andin vivo, we confirm that the subcellular scaffold of the AIS and electrophysiological parameters of labeled cells remain unchanged. We further uncover rapid AIS remodeling following increased network activity in this model system, as well as highly reproduciblein vivolabeling of AIS over weeks. This novel reporter line allows longitudinal studies of AIS modulation and plasticityin vivoin real-time and thus provides a unique approach to study subcellular plasticity in a broad range of applications.
An α-catenin actin-binding domain unfolding mutant designed to lower force-dependent actin-binding promotes epithelial strength but interferes with cell-cell coordination
Authors: Wood Megan , Wang Yuou , Flozak Annette S. , Yemelyanov Alex , Gottardi Cara J. 
Published: February 2023

ABSTRACTα-catenin (α-cat) displays force-dependent binding to actin filaments, but the features of adherens junction structure and function most vulnerable to loss of this allosteric mechanism remain to be generalized across epithelial cell-types. By reconstituting an α-cat F-actin-binding domain unfolding mutant known to exhibit enhanced binding to actin (α-cat-H0-FABD+) into α-cat knock-out Madin Darby Canine Kidney (MDCK) cells, we show that partial loss of the α-cat catch bond mechanism (via an altered H0 α-helix) leads to stronger epithelial sheet integrity with greater co-localization between the α-cat-H0-FABD+mutant and actin. α-cat-H0-FABD+- expressing cells are less efficient at closing scratch-wounds or uniformly packing, suggesting reduced capacity for more dynamic cell-cell coordination. Evidence α-cat-H0-FABD+is equally accessible to the conformationally sensitive α18 antibody epitope as WT α-cat suggests this mutant favors binding to lower tension cortical actin networks. These data suggest signals that reduce the force-sensitivity of the α-cat-cortical actin interaction might improve epithelial monolayer strength through enhanced coupling to lower tension cortical actin networks, but that such association would render α-cat less selective of higher-tension actin networks, extracting a cost for dynamic processes.
Assessment on fuel economy of MoM transport aircraft using empirical approach: modelling with a case study
Authors: Dhara Aishwarya , Muruga Lal Jeyan J.V. 
Published: February 2023

Purpose
This research is associated with the real-time parameters of wide- and narrow-body aircraft to recognize the quantitative relationship framework. This paper aims to find the superiority of aircraft design technology which triggers the reduction in specific fuel consumption (SFC) and economic competitiveness.

Design/methodology/approach
The real case study is performed with 22 middle-of-the-market (MoM) aircraft. This paper develops a fuel burn mathematical model for mid-size transport aircraft by a multi-linear regression approach. In addition, sensitivity analysis is performed to establish the authentication of the fuel burn model.

Findings
The study reveals that the MoM aircraft would be the future aircraft design in terms of better fuel economy and carbon footprint. From the multi-regression analysis, it is observed that the logarithmic regression model is the best fit for estimating the SFC. Moreover, fineness ratio, aspect ratio, gross weight, payload weight fraction, empty weight fraction), fuel weight fraction, payload, wing loading, thrust loading, range, take-off distance, cruise speed and rate of climb are observed as the suitable parameters which provide the best fitness value as 0.9804.

Originality/value
Several existing literature reveals that a few research has been performed on the MoM aircraft with wide-body configuration. Moreover, mathematical modelling on the fuel consumption was insignificantly found. This study examines several parameters which affect the fuel consumption of a wide-body aircraft. A real-case study for design configurations, propulsive systems, performance characteristics and structural integrity parameters of 22 different MoM aircraft are performed. Moreover, multi-regression modelling is developed to establish the relation between SFC and other critical parameters.
Three-dimensional simulation effects of trailing-edge actuation on a morphing A320 wing by means of hybrid turbulence modelling
Authors: Marouf Abderahmane , Hoarau Yannick , Rouchon Jean-François , Braza Marianna 
Published: February 2023

Purpose
This study aims to investigate the effects of electroactive morphing on the Airbus A320 Reduced Scale prototype of the H2020 N° 723402 European Research project smart morphing and sensing (SMS) for aeronautical configurations [1],[2].

Design/methodology/approach
The flow regimes correspond to low subsonic take-off conditions. The morphing is applied through the vibration and slight deformation of the near trailing edge region; respecting the way, this actuation has been applied on the experimental prototype using micro fibre composite actuators. Optimal frequency range has been used, associated with low amplitudes of deformation with the Arbitrary Lagrangian Eulerian methodology. This study used an adapted turbulence modelling with the organised eddy simulation (OES) as well as a hybrid approach delayed detached eddy simulation – with embedded OES (DDES–OES), able to sensitise and keep up the coherent structures development.

Findings
The morphing at an optimal frequency (300 Hz) and amplitude (0.7 mm), applied on a length (3.5 cm) near the trailing edge, has been studied at Reynolds number 1 million and incidence of 10°. The effects on the main flow instabilities and on the turbulent vortex structures are analysed using proper orthogonal decomposition. A modification of the wake structures and a formation of organised rows of vortices along the shear layer are obtained. This leads to a quasi-two-dimensional wake, benefits on the aerodynamic performance and a decrease of the frequency peaks in the spectrum, corresponding to an attenuation of the coherent structures.

Originality/value
This study provides a fundamental understanding of how the actuation modifies the coherent and turbulent vortex structures around the wing and in the wake.
Environmental Kuznets curve, balanced growth, and influencing factors: evidence from economic development in China
Authors: Li Jing 
Published: February 2023

Purpose
The aggregate index and per capita index have different meanings for some countries or regions. CO2 emissions per capita matters for China because of its huge population. Therefore, this study aims to deepen the understanding of Kuznets curve from the perspective of CO2 emissions per capita. In this study, mathematical formulas will be derived and verified.

Design/methodology/approach
First, this study verified the existing problems with the environmental Kuznets curve (EKC) through multiple regression. Second, this study developed a theoretical derivation with the Solow model and balanced growth and explained the underlying principles of the EKC’s shape. Finally, this study quantitatively analyzed the influencing factors.

Findings
The CO2 emission per capita is related to the per capita GDP, nonfossil energy and total factor productivity (TFP). Empirical results support the EKC hypothesis. When the proportion of nonfossil and TFP increase by 1%, the per capita CO2 decrease by 0.041 t and 1.79 t, respectively. The growth rate of CO2 emissions per capita is determined by the difference between the growth rate of output per capita and the sum of efficiency and structural growth rates. To achieve the CO2 emission intensity target and economic growth target, the growth rate of per capita CO2 emissions must fall within the range of [−0.92%, 6.1%].

Originality/value
Inspired by the EKC and balanced growth, this study investigated the relationships between China’s environmental variables (empirical analysis) and developed a theoretical background (macro-theoretical derivation) through formula-based derivation, the results of which are universally valuable and provide policymakers with a newly integrated view of emission reduction and balanced development to address the challenges associated with climate change caused by energy.
Development of convenient flood risk assessment method to community level: the case in the Deduru Oya basin in Sri Lanka
Authors: Wanninayake Sisira Bandara , Nianthi Rekha , Dayarathne Banda Og 
Published: February 2023

Purpose
Floods have been identified as the most frequent and threatening disaster in Sri Lanka amidst an increasing trend of natural and man-made disasters in the world. Subject experts state that disaster risk management should be based on the results of risk assessments, but flood risk management in Sri Lanka is seemingly not based on community-level flood risk assessments. Accordingly, the purpose of this paper is to introduce a community-level flood risk assessment method to the local context of Sri Lanka.

Design/methodology/approach
The sample (n = 425) for the study was selected using the stratified random sampling method, and the Deduru Oya basin was selected as the study area. The risk assessment model introduced by Bollin et al. (2003) was used for the current study, but with some modifications. Accordingly, 16 variables were selected for the risk assessment. Descriptive data analysis methods were used in the study.

Findings
Community-level flood risk assessment method was introduced. Variable index, flood risk index and flood risk map were developed for the study area. The Grama Niladari Divisions (GNDs) were grouped into five categories from very high risk to very low risk. The GNDs named Wirakumandaluwa, Thimbilla, Deduru Oya, Bangadeniya and Elivitiya were ranked as the most flood-risk GNDs, respectively.

Originality/value
This paper produces a flood risk assessment method for the local context. Flood risk in the study area was assessed based on people’s perceptions. Accordingly, the flood risk index and flood risk map for the study area were developed based on the empirical data. GNDs were ranked based on the flood risk index.
Market-based organizational learning, pricing capability and business performance: the moderating effects of coordination mechanism and environmental dynamism
Authors: Ranjan Piyush , Nayak Jogendra Kumar 
Published: February 2023

Purpose
This study aims to present a conceptual framework for understanding the dual orientations of market-based organizational learning (MBOL), namely, market orientation (MO) and learning orientation (LO), in the development of pricing capability (PC) with the goal of improving business performance (BP). This framework further explores the moderating effects of coordination mechanism (CM) and environmental dynamism (ED) on the PC–BP link and the mediating role of PC on the relationship between MBOL and BP.

Design/methodology/approach
This study applied the partial least squares structural equation modeling on survey data from 298 Indian small- and medium-sized enterprises (SMEs) operating in manufacturing and service sectors.

Findings
The findings indicate that MBOL significantly contributes to PC development, which in turn improves BP. Interestingly, PC acts as a partial mediator in the MO–BP link, as well as LO–BP link. Moreover, CM and ED strengthen the effect of PC on BP. Finally, MO and LO have substantial and distinct effects on PC and BP.

Research limitations/implications
This study examines only one market-related capability, i.e. PC, considers multi-industry SMEs rather than specific large industries and uses cross-sectional instead of longitudinal data.

Practical implications
These findings are crucial from managerial standpoints because SMEs need to understand the MBOL dimensions, including MO and LO, and their significance in improving PC and BP.

Originality/value
Understanding how MBOL adoption contributes to superior performance is critical, but research in the SMEs context is still lacking. This study addresses a research gap by examining the impact of MBOL on BP, both directly and indirectly, through PC in the context of SMEs.
Synthesis of critical factors influencing indoor environmental quality and their impacts on building occupants health and productivity
Authors: Mewomo Modupe Cecilia , Toyin James Olaonipekun , Iyiola Comfort Olubukola , Aluko Olusola Raphael 
Published: February 2023

Purpose
The present shift and change in the human lifestyle across the world are undeniable. Currently, individuals spend a substantial amount of time indoors due to the global COVID-19 pandemic that strikes the entire world. This change in human lifestyle has devastating effects on human health and productivity. As a result, the influence of indoor environmental quality (IEQ) on the health and productivity of building users becomes a critical field of research that requires immediate attention. As a result, the purpose of this study is to review the state-of-the-art literature by establishing a connection between the factors that influence health and productivity in any given indoor environment.

Design/methodology/approach
The methodology involves a thorough review of selected published journals from 1983 to 2021, and the result was analysed through content analysis. The search included journal articles, books and conference proceedings on the critical factors influencing IEQ and their impact on building occupants, which was sourced from different databases such as ScienceDirect, Taylor, GoogleScholar and Web of Science.

Findings
The findings from the 90 selected articles revealed four critical factors influencing the quality of the indoor environment and are categorised into; indoor air quality, indoor thermal comfort, visual comfort and acoustic comfort. The findings suggested that when developing a system for controlling the quality of the indoor environment, the indoor air quality, indoor thermal comfort, visual comfort and acoustic comfort should be taken into account.

Originality/value
The indoor environment deeply impacts the health of individuals in their living and work environments. Industry must have a moral responsibility to provide health facilities in which people and workers feel satisfies and give conditions for prosperity. Addressing these essential aspects will not only help the decision-making process of construction professionals but also encourages innovative construction techniques that will enhance the satisfaction, wellness and performance of building occupants.