bluuebunny/crossref_metadata_2025_split huggingface dataset:
A novel histological staging of hippocampal sclerosis that is evident in gray matter loss in vivo
Authors: Ortega‐Cruz Diana , Uceda‐Heras Alicia , Iglesias Juan Eugenio , Zea‐Sevilla María Ascensión , Strange Bryan , Rabano Alberto 
Published: January 2023

AbstractIntroductionHippocampal sclerosis of aging (HS) is defined by end‐stage histological findings, strongly associated with limbic‐predominant age‐related TAR DNA‐binding protein 43 (TDP‐43) encephalopathy (LATE). We aimed to characterize features of early HS to refine the understanding of its role within combined pathology.MethodsWe studied 159 brain donations from the multimodal Vallecas Alzheimer's Center Study. A staging system (0 to IV) was developed to account for HS progression and analyzed in relation to pre‐mortem cognitive and magnetic resonance imaging (MRI) data.ResultsOur HS staging system displayed a significant correlation with disease duration, cognitive performance, and combined neuropathologies, especially with LATE. Two‐level assessment along the hippocampal longitudinal axis revealed an anterior‐posterior gradient of HS severity. In vivo MRI showed focally reduced hippocampal gray matter density as a function of HS staging.DiscussionThe association of this staging system with clinical progression and structural differences supports its utility in the characterization and potential in vivo monitoring of HS.Highlights
The definition of hippocampal sclerosis of aging (HS) is currently limited to an end‐stage pathological fingerprint.
We characterize early HS histological features to define a complete staging system.

The proposed staging displays a parallel but not identical progression to limbic‐predominant age‐related TAR DNA‐binding protein 43 (TDP‐43) encephalopathy (LATE).

The proposed staging
also reflects the expected demographic and cognitive differences associated with HS.
In vivo magnetic resonance imaging (MRI) showed focal hippocampal gray matter loss as a function of HS staging.
Complementary Design in Multicomponent Electrocatalysts for Electrochemical Nitrogen Reduction: Beyond the Leverage in Activity and Selectivity
Authors: Qiang Siyu , Wu Fan , Yu Jianyong , Liu Yi‐Tao , Ding Bin 
Published: January 2023

AbstractElectrochemical nitrogen reduction reaction (eNRR) is promising in place of the Haber–Bosch process for artificial N2fixation. However, the high activity and selectivity of eNRR are challenging to achieve simultaneously due to the scaling relations. Such “leverage” between activity and selectivity has severely restricted eNRR. To overcome this bottleneck, the complementary design of electronic structures in multicomponent electrocatalysts has been recently pursued, aiming to maximize the advantages of each component and optimize the multistep reactions, which has stood at the cutting edge in this aspect. Here, we present a minireview of the design, performance, and mechanism of multicomponent electrocatalysts with complementary electronic structures. We particularly emphasize the interactions between N2and elements fromd‐,p‐, ands‐blocks, which are essential for understanding how these electrocatalysts are beyond the “leverage” between activity and selectivity.
Ultra‐schnelle Präparation großflächiger Graphdiin‐basierter Membranen mittels Alkin‐Oberflächenmodifizierung
Authors: Yang Xingda , Qu Zhou , Li Sen , Peng Manhua , Li Chunxi , Hua Ruimao , Fan Hongwei , Caro Jürgen , Meng Hong 
Published: January 2023

AbstractGraphdiine (engl. graphdiynes=GDYs) sind zweidimensionale graphenähnliche Kohlenstoffsysteme und gelten aufgrund ihrer einzigartigen physikalisch‐chemischen Eigenschaften als potenzielles Membranmaterial. Die Maßstabvergrößerung der Präparation von GDY‐Membranen ist jedoch eine technologische Herausforderung. Wir berichten hier über eine einfache und effiziente alkinierte oberflächenvermittelte Strategie zur Herstellung von wasserstoffsubstituierten Graphdiin[1c] (HsGDY)‐Membranen auf kommerziellen Aluminiumoxidrohren, die zuvor Alkin‐modifiziert wurden. Die Oberflächenalkinierung der Keramik erlaubt eine oberflächenbegrenzte Kupplungsreaktion in Gegenwart eines Kupferkatalysators und ermöglicht so das epitaktische Wachstum von HsGDY im Nanomaßstab. Eine kontinuierliche und ultradünne HsGDY‐Membran (∼100 nm) kann innerhalb von 15 min hergestellt werden. Die resultierenden Membranen weisen eine sehr hohe Molekularsiebung zusammen mit hervorragender Wasserpermeanz (ca. 1100 L m−2 h−1 MPa−1) auf und zeigen aufgrund der superhydrophilen Oberfläche und hydrophoben Porenwände ein stabiles Trennverhalten in der Cross‐Flow‐Nanofiltration.
Understanding Synergistic Catalysis on Cu‐Se Dual Atom Sites viaOperandoX‐ray Absorption Spectroscopy in Oxygen Reduction Reaction
Authors: Sun Zhiguo , Zhang Huijuan , Cao Linlin , Liu Xiaokang , Wu Dan , Shen Xinyi , Zhang Xue , Chen Zihang , Ru Sen , Zhu Xiangyu , Xia Zhiyuan , Luo Qiquan , Xu Faqiang , Yao Tao 
Published: January 2023

AbstractThe construction and understanding of synergy in well‐defined dual‐atom active sites is an available avenue to promote multistep tandem catalytic reactions. Herein, we construct a dual‐hetero‐atom catalyst that comprises adjacent Cu‐N4and Se‐C3active sites for efficient oxygen reduction reaction (ORR) activity.OperandoX‐ray absorption spectroscopy coupled with theoretical calculations provide in‐depth insights into this dual‐atom synergy mechanism for ORR under realistic device operation conditions. The heteroatom Se modulator can efficiently polarize the charge distribution around symmetrical Cu‐N4moieties, and serve as synergistic site to facilitate the second oxygen reduction step simultaneously, in which the key OOH*‐(Cu1‐N4) transforms to O*‐(Se1‐C2) intermediate on the dual‐atom sites. Therefore, this designed catalyst achieves satisfied alkaline ORR activity with a half‐wave potential of 0.905 V vs. RHE and a maximum power density of 206.5 mW cm−2in Zn‐air battery.
Macromolecular Information Transfer
Authors: Samokhvalova Svetlana , Lutz Jean‐François 
Published: January 2023

AbstractMacromolecular information transfer can be defined as the process by which a coded monomer sequence is communicated from one macromolecule to another. In such a transfer process, the information sequence can be kept identical, transformed into a complementary sequence or even translated into a different molecular language. Such mechanisms are crucial in biology and take place in DNA→DNA replication, DNA→RNA transcription and RNA→protein translation. In fact, there would be no life on Earth without macromolecular information transfer. Mimicking such processes with synthetic macromolecules would also be of major scientific relevance because it would open up new avenues for technological applications (e.g. data storage and processing) but also for the creation of artificial life. In this important context, this minireview summarizes recent research about information transfer in synthetic oligomers and polymers. Medium‐ and long‐term perspectives are also discussed.
Synthesis and Reactivity of a Cyclooctatetraene‐Like Polyphosphorus Ligand Complex [Cyclo‐P8]
Authors: Riesinger Christoph , Dielmann Fabian , Szlosek Robert , Virovets Alexander V. , Scheer Manfred 
Published: January 2023

AbstractThe thermolysis of Cp′′′Ta(CO)4 with white phosphorus (P4) gives access to [{Cp′′′Ta}2(μ,η2 : 2 : 2 : 2 : 1 : 1‐P8)] (A), representing the first complex containing a cyclooctatetraene‐like (COT) cyclo‐P8 ligand. While ring sizes of n >6 have remained elusive for cyclo‐Pn structural motifs, the choice of the transition metal, co‐ligand and reaction conditions allowed the isolation of A. Reactivity investigations reveal its versatile coordination behaviour as well as its redox properties. Oxidation leads to dimerization to afford [{Cp′′′Ta}4(μ4,η2 : 2 : 2 : 2 : 2 : 2 : 2 : 2 : 1 : 1 : 1 : 1‐P16)][TEF]2 (4, TEF=[Al(OC{CF3}3)4]−). Reduction, however, leads to the fission of one P−P bond in A followed by rapid dimerization to form [K@[2.2.2]cryptand]2[{Cp′′′Ta}4(μ4,η2 : 2 : 2 : 2 : 2 : 2 : 2 : 2 : 1 : 1 : 1 : 1‐P16)] (5), which features an unprecedented chain‐type P16 ligand. Lastly, A serves as a P2 synthon, via ring contraction to the triple‐decker complex [{Cp′′′Ta}2(μ,η6 : 6‐P6)] (B).
Multifunctional poly(vinylidene fluoride–co‐hexafluoropropylene) ‐ zinc stannate nanocomposite for high energy density capacitors and piezo‐phototronic switching
Authors: Kumar Ajay , Mandal Dipankar 
Published: January 2023

AbstractElectroactive multifunctional nanocomposite films of poly(vinylidene fluoride – co ‐ hexafluoropropylene) (P(VDF‐HFP)) and different zinc stannate (ZnSnO3) filler concentrations were processed by hot pressing to achieve multilayer structure formation. A notable increase in the dielectric constant (K ≈ 30 at 1 kHz) was achieved in the three‐layered hot‐pressed composite film. The resultant relaxation time, that is, the time required to reach the remnant polarization (Pr) from saturation polarization (Ps) of hot‐pressed films reduced to 1/20th time to that of its single‐layer film. Furthermore, ferroelectric loop data of hot‐pressed structure shows the lower value of remnant polarization (0.20 μC/cm2) as compared to nanocomposite film (0.28 μC/cm2) under similar measurement conditions, which further leads to an increase in energy density (2900 mJ/cm3 at 250 MV/m vs 500 mJ/cm3 at 150 MV/m). The study shows the dependency of energy storage and remnant polarization on the relaxation time of dielectric that can be further explored to enhance the energy density of dielectric capacitors. Thereafter, the multifunctional nature of nanocomposite film is shown by coupling semiconducting, light and piezoelectric effects in piezo‐phototronic switching under tensile and compressive strain in dark and light conditions.
In Situ Reconstructed Mo‐doped Amorphous FeOOH Boosts the Oxygen Evolution Reaction
Authors: Jia Zhichao , Lyu Xiang , Zhao Mingsheng , Dang Jianan , Zhu Linge , Guo Xiaowei , Wang Xiaobing , Bai Zhengyu , Yang Lin 
Published: January 2023

AbstractDeveloping a fast and highly active oxygen evolution reaction (OER) catalyst to change energy kinetics technology is essential for making clean energy. Herein, we prepare three‐dimensional (3D) hollow Mo‐doped amorphous FeOOH (Mo‐FeOOH) based on the precatalyst MoS2/FeC2O4 via in situ reconstruction strategy. Mo‐FeOOH exhibits promising OER performance. Specifically, it has an overpotential of 285 mV and a durability of 15 h at 10 mA cm−2. Characterizations indicate that Mo was included inside the FeOOH lattice, and it not only modifies the electronic energy levels of FeOOH but also effectively raises the inherent activity of FeOOH for OER. Additionally, in situ Raman analysis indicates that FeC2O4 gradually transforms into the FeOOH active site throughout the OER process. This study provides ideas for designing in situ reconstruction strategies to prepare heteroatom doping catalysts for high electrochemical activity.
Enhancing recombinant protein and viral vector production in mammalian cells by targeting the YTHDF readers of N6‐methyladenosine in mRNA
Authors: Lao Nga , Barron Niall 
Published: January 2023

AbstractN6‐methyladenosine (m6A) is the most abundant internal modification on eukaryotic mRNA and has been implicated in a wide range of fundamental cellular processes. This modification is regulated and interpreted by a set of writer, eraser, and reader proteins. To date, there have been no reports on the potential of mRNA epigenetic regulators to influence recombinant protein expression in mammalian cells. In this study, the potential of manipulating the expression of the m6A YTH domain‐containing readers, YTHDF1, 2 and 3 to improve recombinant protein yield based on their role in regulating mRNA stability and promoting translation were evaluated. Using siRNA‐mediated gene depletion, cDNA over‐expression, and methylation‐specific RNA immunoprecipitation, it is demonstrated that (i) knock‐down of YTHDF2 enhances (~2‐fold) the levels of recombinant protein derived from GFP and EPO transgenes in CHO cells; (ii) the effects of YTHDF2 depletion on transgene expression is m6A‐mediated; and (iii) YTHDF2 depletion, or over‐expression of YTHDF1 increases viral protein expression and yield of infectious lentiviral (LV) particles (~2–3‐fold) in HEK293 cells. We conclude that various transgenes can be subjected to regulation by m6A regulators in mammalian cell lines and that these findings demonstrate the utility of epitranscriptomic‐based approaches to host cell line engineering for improved recombinant protein and viral vector production.
An effective and stability‐indicating method development and optimization utilizing the Box–Behnken design for the simultaneous determination of acetaminophen, caffeine, and aspirin in tablet formulation
Authors: Yenda Parvateesam , Katari Naresh Kumar , Ettaboina Santhosh Kumar , Satheesh Balasubramanian , Muchakayala Siva Krishna , Gundla Rambabu 
Published: January 2023

AbstractAnalytical techniques must be sensitive, specific, and accurate to assess the active pharmaceutical ingredients in pharmaceutical dosage forms. The quality‐by‐design (QbD) application has proven to be a practical method for magnifying HPLC operations. This article discusses the successfully developed QbD‐based stability‐indicative LC method for evaluating acetaminophen, caffeine, and aspirin (ASP) in tablet dosage form. To achieve the necessary chromatographic separation, Milli‐Q water, methanol, and glacial acetic acid were employed in the following ratios: 63:35:2 (v/v/v) for mobile phase A and 18:80:2 (v/v/v) for mobile phase B. The flow rate, column temperature, and detecting wavelength were 1.0 ml/min, 40°C, and 275 nm, respectively, and an InertSustain C18 analytical column (150 × 4.6 mm, 3 μm) was used. Linearity was between 10.0 and 150.0 μg/ml for ASP and acetaminophen and between 2.6 and 39.0 μg/ml for caffeine. The accuracy findings were more than 97%, and the correlation coefficient for all three components was found to be greater than 0.999. The validated HPLC method yielded reliable and accurate results. ASP was shown to be vulnerable to both acid and alkaline hydrolysis in the forced degradation study. The described method is capable of separating the degradants produced during stress testing and is regarded as stability indicating. The proposed method can be used for a wider range of other formulations with an appropriate diluent selection and sample preparation procedure optimization.
Studying Reversible Protein Post‐translational Modification through Co‐translational Modification
Authors: Wu Dan , Liu Tao 
Published: January 2023

AbstractUnderstanding the post‐translational modifications of targeted proteins is of great significance for manipulating the physiological processes of eukaryotes. Chemical biology tools have been used to investigate the biological roles of those post‐translational modifications at particular sites, especially genetic code expansion technology, which can also be combined with the concept of synthetic biology to generate a genetically modified organism with a synthetic auxotroph for co‐translational modification components. In this concept, we will introduce applications, limitations, and perspectives of genetic code expansion technology for studying post‐translational modification based on recent progresses. Future perspectives of genetically modified organisms also will be discussed in regard to the application of post‐translational modification research.
Comparison of the effects of buffalo LIF and mouse LIF on the in vitro culture of buffalo spermatogonia
Authors: Liu Ya Ru , Li Wang Chang , Hu Jia Hao , Li Qi Qi , Zhang Ya Ping , Lu Ke Huan , Xu Hui Yan , Liang Xing Wei , Lu Yang Qing , Yang Xiao Gan 
Published: January 2023

AbstractLeukemia inhibitory factor (LIF) is an important growth factor that supports the culture and maintenance of spermatogonial stem cells (SSCs) by suppressing spontaneous differentiation. Different LIF sequences may lead to differences in function. The protein sequences of buffalo LIF and mouse LIF differed by 65.5% according to MEGA software analysis. The PB‐LIF‐GFP‐Puro vector was constructed, and the CHO‐K1 cell line was established. The final LIF protein concentration in the CHO‐K1 cell culture medium was approximately 4.268 ng/mL. Here, we report that buffalo LIF effectively maintains the self‐renewal of buffalo spermatogonia during culture. Buffalo spermatogonia were cultured in conditioned medium containing no LIF (0 ng/mL), mouse LIF (1 ng/mL), mouse LIF (10 ng/mL), or buffalo LIF (1 ng/mL). Furthermore, the effects of mouse LIF and buffalo LIF culture on the maintenance of buffalo spermatogonia were determined by analyzing cell colony formation, quantitative real‐time polymerase chain reaction, cell immunofluorescence, and cell counting. The buffalo LIF (1 ng/mL) group showed similar maintenance of the proliferation of buffalo spermatogonia to that in the mouse LIF (10 ng/mL) group. These results demonstrated that the proliferation of buffalo spermatogonia can be maintained in vitro by adding a low dose of buffalo LIF. This study provides a foundation for the further optimization of in vitro buffalo SSC culture systems.
Novel dual guiding catheter technique for kissing balloon inflation in percutaneous coronary intervention for bifurcated chronic total occlusive lesion
Authors: Kawamura Yota , Yoshimachi Fuminobu , Kasai Satoshi , Ikari Yuji 
Published: January 2023

AbstractTraditionally, two bigger‐sized guiding catheters (GCs) via the femoral artery have been used in percutaneous coronary intervention (PCI) for chronic total occlusion (CTO). However, those GCs are associated with an increased incidence of bleeding. Therefore, the use of smaller GCs (e.g., 5‐Fr) may lessen the invasiveness of this procedure. However, the use of 5‐Fr GCs in PCI is complicated by device limitations. A novel technique was developed to overcome the challenges with regard to kissing balloon inflation. This approach involves simultaneous balloon inflation through a dual 5‐Fr GC technique for CTO‐PCI. In our hospital, this technique was successfully performed in 9 cases of CTO with 10 bifurcated lesions (including four left main‐related bifurcations). During the study period, there was no requirement for an additional invasive procedure or occurrence of complications related to the PCI procedure. This technique may be a good option for performing KBI during CTO‐PCI through the use of two 5‐Fr GCs.
Heart rate response during cardiopulmonary exercise in the denervated heart
Authors: Ono Ryohei , Iwahana Togo , Kato Hirotoshi , Kobayashi Yoshio 
Published: January 2023

AbstractThe patients after heart transplantation usually present resting tachycardia, a slower increase in heart rate (HR) at the onset of exercise, a blunted chronotropic response to exercise in general, maximal HR being attained in the recovery period rather than at peak exercise, and a slower decline in HR after exercise.
Sustainable development of supplier performance. An empirical analysis of relationship characteristics in the automotive sector
Authors: Naffin Jule , Klewitz Johanna , Schaltegger Stefan 
Published: January 2023

AbstractSustainability performance of suppliers has become increasingly important for both corporate practice and research. While the importance of relationships between the focal company and its suppliers has been acknowledged for improving sustainability performance of suppliers, little is known about the role of relation specific factors in practice. This research fills this gap by empirically analyzing the relevance of relationspecific factors for the sustainability performance of suppliers based on a unique data set of 736 first‐tier suppliers of a German original equipment manufacturer in the automotive industry. The analysis reveals that the sustainability performance of suppliers relates positively to transaction volume and relationship length as specific forms of relation specific characteristics as well as strategic partnership as a proxy of incentivized, in‐depth cooperation and knowledge exchange. Each of the investigated relation‐specific characteristics show a link with either environmental or with social performance. Conclusions are drawn for research and management.
Cytology as the guiding light: Prompt cytomorphologic diagnosis of cystic metastases from a jejunal neuroendocrine tumor masquerading as hepatic abscesses
Authors: Tom Jesty Pullattu , Singh Aditya , Gupta Parikshaa , Gupta Nalini , Basher Rajender Kumar , Suri Vikas 
Published: January 2023

AbstractHepatic cystic lesions are commonly seen in radiology and can occur due to infective or neoplastic causes. It is imperative to determine the precise nature of these hepatic cysts owing to significant therapeutic and prognostic differences. Hepatic metastases from gastrointestinal neuroendocrine tumors (NETs) mostly present as solid hypervascular lesions. Cystic presentation of the metastatic NETs is extremely rare. A 64‐year‐old woman presented with an abdominal lump of 3 months duration and high‐grade fever from the last 2 days. An ultrasound abdomen revealed multiple hypoechoic liver lesions suggestive of liver abscesses. An ultrasound‐guided fine‐needle aspiration (FNA) from the cystic liver lesions revealed a NET confirmed by immunocytochemistry. The Ki‐67 index was 5%; hence, a final diagnosis of metastatic NET, grade 2, was given with advice to work up for localizing the primary tumor. Subsequently, a contrast‐enhanced computerized tomography of the abdomen revealed a polypoidal lesion in the proximal jejunum, which showed intense expression on the whole‐body DOTANOC scan (SUVmax‐76.5), indicating it to be the likely primary tumor along with somatostatin receptor‐expressing multiple lymph nodes and hypodense liver lesions. Thus, a final diagnosis of a jejunal NET with abdominal lymph node and liver metastases was made. The present report highlights the importance of FNA as a reliable modality in diagnosing metastatic NETs presenting as unusual cystic hepatic metastases masquerading as liver abscesses. Additionally, the cell block helps confirm the cytologic diagnosis and enables upfront tumor grading, thereby helping in prognostication and therapeutic decision‐making.
Design, synthesis, and antitumor evaluation of trimethoxyflavonoid with arylurea structure against hepatocellular carcinoma
Authors: Sun Xueyan , Zhao Yin , Zhao Jingduo , Xie Zhizhong , Lei Xiaoyong , Liu Xingyun , Li Yong , Huang Sheng , Wang Zhe , Tang Guotao 
Published: January 2023

AbstractSimultaneous targeting of tumor vasculature and inhibitors of tumor cell glycolysis may be a promising antitumor strategy. Here, we reported the total synthesis and biological evaluation of A‐ring arylurea flavonoid derivatives with B‐ring trimethoxy group, which exhibited potent antitumor activity against a variety of tumor cells in vitro. Most of the derivatives showed in vitro antitumor activity on HepG‐2, HGC‐27, MDA‐MB‐231, and A549 cells. Among them, compounds 8e, 8f, 8g, 8h, 8j, and 8l also exhibited significant anti‐proliferation effects on liver tumor cell subtypes BEL‐7402 and SMMC‐7721. Compound 8l had the lowest IC50 value (5.61 ± 0.39 μM) on HepG‐2 cells, and showed the effects of inhibiting colony formation, arresting the cell cycle in G0/G1 phase, and inducing apoptosis in a concentration‐dependent manner. In addition, the toxicity of compound 8l on human normal cells LO2 and GES‐1 was lower than that of sorafenib. The inhibitory effects of compound 8l on the expression of glycolytic rate‐limiting enzymes HKII, PFK‐1, PKM2 and vascular endothelial growth factor were further evaluated. Corresponding reduction in intracellular lactate was also detected after compound 8 treatment. Our results support an antitumor strategy targeting tumor vasculature and glycolysis to discover and develop a new generation of antitumor drugs.