bluuebunny/crossref_metadata_2025_split huggingface dataset:
Rampant Dispersal Without Gene‐Flow: Reproductively and Geographically Isolated Lineages of the Supertramp Lizard Lamprolepis smaragdina Permeate the Lesser Sunda Archipelago
Authors: Reilly Sean B. , Kaiser Hinrich , Karin Benjamin R. , Arifin Umilaela , Stubbs Alexander L. , Arida Evy , Hamidy Amir , Iskandar Djoko T. , McGuire Jimmy A. 
Published: January 2025

ABSTRACTAimWe investigated the biogeographical patterns of a widespread arboreal lizard found throughout Wallacea and the western Pacific islands. Wallacea contains longstanding oceanic currents that flow from the Pacific Ocean into the Indian Ocean, known as the Indonesian Throughflow. Using genetic data, we aim to estimate the timing and sequence of island colonisation in the Lesser Sunda archipelago of southern Wallacea, and to determine the proportion of dispersal events that may have been aided by ocean currents. Levels of gene flow are also explored in the context of island arrangement, past connectivity (ex. Pleistocene land bridges), and ocean currents.LocationSouthern Wallacea (Indonesia, Timor‐Leste).TaxonEmerald Tree Skink (Lamprolepis smaragdina).MethodsWe estimated a mitochondrial DNA phylogeny from southern Wallacean populations to identify divergent populations and to guide sample selection for genomic‐scale data collection. These genomic data were used to estimate (1) phylogenetic relationships of island populations using both concatenated Maximum Likelihood, summary coalescent and multispecies coalescent methods, (2) levels of genetic connectivity between lineages using genetic clustering and migration analyses, and (3) to reconstruct their dispersal history using ancestral range reconstruction analysis.ResultsWe found that the Emerald Tree Skinks of southern Wallacea diverged from a population on Peleng Island ~1.25 Ma and that Lesser Sunda populations diverged between ~1 and 0.4 Ma. MtDNA data are insufficient to estimate the relationships of each population and find one island to be paraphyletic and two to be polyphyletic. Genomic data find that Timor Island is polyphyletic while all other islands are recovered as monophyletic. Gene flow is minimal or absent across the archipelago, even between islands that become periodically land bridged. More than half of the estimated over‐water dispersal events occurred perpendicular to or against ocean currents.Main ConclusionsDespite this species' over‐water dispersal abilities island populations are genetically isolated from one another, even on adjacent islands that become periodically land bridged. This suggests that species formation is complete. The inferred dispersal history involved many paths that would be difficult to complete given the direction of ocean currents, indicating that historical ocean currents are more dynamic than currently understood.
Clinical Practice Guidelines for Topical NSAIDs in the Treatment of Sports Injuries
Authors: Xiong Yan , Liu Yan , Zhou Jingbin , Shang Xiliang , He Hongchen , Li Guoping , Chen Shiyi , Li Jian 
Published: January 2025

ABSTRACTTopical nonsteroidal anti‐inflammatory drugs (NSAIDs) are commonly used to treat sports injuries, but evidence‐based medical guidance for their standardized and rational use is lacking. This guideline working group identified clinically important issues, obtained the full opinions of patients and clinical staff, and discussed them with the expert group. Based on evidence from the literature, the “clinical practice guidelines for topical NSAIDs in the treatment of sports injuries” were formulated following the methods and principles of international guidelines. According to these guidelines, 7 clinical concerns were ultimately selected, and 22 recommendations were formed. These included the status, indications, contraindications, efficacy, combined application, use in special populations, adverse reactions, and countermeasures of topical NSAIDs in the treatment of sports injuries. The purpose of these guidelines is to provide evidence‐based recommendations for practitioners in the fields of orthopedics, sports medicine, rehabilitation medicine, and sports science, as well as other fields, in the treatment of sports injuries to promote more standardized and rational use of topical NSAIDs.
Neuronal TRPV1‐CGRP axis regulates peripheral nerve regeneration through ERK/HIF‐1 signaling pathway
Authors: Che Huiling , Du Yu , Jiang Yixuan , Zhu Zhanfeng , Bai Mingxuan , Zheng Jianan , Yang Mao , Xiang Lin , Gong Ping 
Published: January 2025

AbstractSevere trauma frequently leads to nerve damage. Peripheral nerves possess a degree of regenerative ability, and actively promoting their recovery can help restore the sensory and functional capacities of tissues. The neuropeptide calcitonin gene‐related peptide (CGRP) is believed to regulate the repair of injured peripheral nerves, with neuronal transient receptor potential vanilloid type 1 (TRPV1) potentially serving as a crucial upstream factor. In this study, we established a mouse model of sciatic nerve (SN) crush injury and found that intrathecal injection of capsaicin (Cap) activated the neuronal TRPV1‐CGRP axis, thereby promoting SN repair. Conversely, the application of capsazepine (Cpz), which inhibits the neuronal TRPV1‐CGRP axis, delayed SN repair. Local restoration of CGRP expression at the injury site enhanced the repair process. In vitro experiments, we employed the rat Schwann cell (SC) line RSC96 to establish an indirect co‐culture model of neurons and SCs. We observed that the proliferation, migration, expression of myelination‐associated proteins, and neurotrophic secretion functions of RSC96 cells are positively correlated with the degree of activation of neuronal TRPV1. Inhibition of neuronal TRPV1, followed by the restoration of CGRP levels, improved these functions in RSC96 cells. Furthermore, activation of the neuronal TRPV1‐CGRP axis resulted in an upregulation of extracellular signal‐regulated kinases 1/2 (ERK1/2) phosphorylation levels and an increase in hypoxia‐inducible factor 1α (HIF‐1α) accumulation in RSC96 cells, thereby promoting their proliferation and migration. In summary, this study demonstrates that neuronal TRPV1‐CGRP axis can regulate biological behavior of SCs and axon regeneration by activating the ERK/HIF‐1 signaling pathway following peripheral nerve injury. This finding clarifies the role of CGRP in neuroregulatory networks and provides a novel reference point for the development of drugs and biomaterials for treating nerve damage.image
AENK ameliorates cognitive impairment and prevents Tau hyperphosphorylation through inhibiting AEP‐mediated cleavage of SET in rats with ischemic stroke
Authors: Liu Yi , Liu Wei , Guo Cuiping , Bao Jian , Zhang Huiliang , Li Wensheng , Li Youwei , Gui Yurang , Zeng Yi , Wang Xiaochuan , Peng Caixia , Shu Xiji , Liu Rong 
Published: January 2025

AbstractBrain damage induced by ischemia promotes the development of cognitive dysfunction, thus increasing the risk of dementia such as Alzheimer's disease (AD). Studies indicate that cellular acidification‐triggered activation of asparagine endopeptidase (AEP) plays a key role in ischemic brain injury, through multiple molecular pathways, including cleavage of its substrates such as SET (inhibitor 2 of PP2A, I2PP2A) and Tau. However, whether direct targeting AEP can effectively prevent post‐stroke cognitive impairment (PSCI) remains unanswered. Here, we explored the therapeutic effect and underlying mechanism of the AEP inhibitor AENK on cognitive impairment of the rats with middle cerebral artery occlusion (MCAO) and on neuronal damage in cultured primary neurons exposed to oxygen and glucose deprivation (OGD). We found that the administration of AENK significantly reduces activated AEP levels in ischemic rat brains, attenuates cognitive deficits, and rescues synaptic dysfunction. For the mechanism, with AEP inhibition, cleavage of SET, inhibition of protein phosphatase 2A (PP2A), and Tau hyperphosphorylation resulted from PP2A inhibition, were all completely or partially reversed. In primary neurons, AENK effectively prevents AEP activation, SET cleavage and cytoplasmic retention, tau hyperphosphorylation and synaptic damage induced by OGD. We conclude that AENK ameliorates cognitive impairment and prevents tau hyperphosphorylation, through inhibiting AEP‐mediated cleavage of SET in ischemic brain injury, and direct inhibition of AEP might be a potential therapeutic strategy for preventing synaptic damage and cognitive impairment after stroke.image
Synthetic Dermal Fillers in Treating Acne Scars: A Comparative Systematic Review
Authors: Albargawi Salma 
Published: January 2025

ABSTRACTBackgroundAcne is a common condition observed in adolescents and in most severe acne the scars develop. There are numerous treatment options for acne scars. However, no standardized guidelines have been established to guide physicians in the optimal treatment of acne scars.AimsThe objective of this systematic review is to evaluate the existing evidence on various fillers used for the treatment of acne scars and to compare their effectiveness with one another.MethodsThe study was designed following PRISMA guidelines, and the information was retrieved in May 2024 using the PubMed database and ClinicalTrials.gov registry. The inclusion criteria were that studies involving patients of any age or gender with acne scars of any type treated with synthetic dermal fillers, and studies published in English. The exclusion criteria were studies with less than 10 participants and studies that did not use synthetic dermal fillers. To assess the risk of bias in the included studies, the Cochrane Collaboration's Risk of Bias tool was used for randomized controlled trials, and in observational studies, the Newcastle–Ottawa Scale was used.ResultsTwenty‐six studies were included with a total of 1121 participants. Fourteen studies evaluated HA on 372 subjects, five studies focused on PMMA on 305 subjects, four on CaHA on 392 subjects, two on PLLA on 42 subjects, and one on PCL on 10 subjects.ConclusionsMost of the studies included in this review were of low quality, as indicated by their scores on quality assessments, lack of high‐quality RCTs, and small sample sizes. Future research should focus on conducting randomized, controlled, split‐face studies with an adequate number of participants and a detailed examination of different scar subtypes.
The Impact of an Orofacial Muscle Strengthening Program on Temporomandibular Muscles and on the Performance of Wind Instrumentalists
Authors: de Sá Joana Salomé Nunes , da Silva Cavalheiro Luis Manuel Neves , Silva Carla Sofia Duarte Matos 
Published: January 2025

ABSTRACTIntroductionPlaying a wind instrument is one of the most complex tasks for the musculoskeletal system.ObjectiveVerify the effectiveness of a strengthening exercise programme on musical performance and the strength of the temporomandibular muscles.MethodsThe sample was 60 participants (36 men; 24 women), with a mean age of 24.67 ± 13.06 years, divided into experimental group (EG) (16 brass; 15 woodwind) and control group (CG) (15 brass; 14 woodwind). The initial assessment (T0) consisted of a sociodemographic questionnaire, the EQ‐5D‐5L, an assessment of maximum bite force and endurance time and sound recording, before (T0) and after a musical practice of 8.5 min (T1). The EG, during 5 weeks, underwent 10 sessions of a temporomandibular muscle strengthening program, consisting of a series of 25 repetitions of isometric bite contraction, followed by a reassessment (T2) and sound recording (T2 and T3).ResultsStatistically significant differences were found between groups in maximum bite force between T0 and T2, explained by the improvement in the EG (p = 0.014, bilaterally). We found no differences in endurance. In musical performance, statistically significant differences were found in the mean intensity values. There was an improvement in performance in the brass EG, with statistically significant differences in the high‐piano note (p = 0.025) and in the woodwind EG all the notes were played at a significantly lower intensity (p ≤ 0.05), compared to the CG, where all the notes increased in intensity.ConclusionThe isometric exercise improves the maximum bite force, accompanied by an improvement in the high note's performance in brass and greater sound stability in woodwinds.
Interactive Effects of COVID‐19 Infection and Psychological Factors on Oral Health‐Related Quality of Life in Myanmar
Authors: Thwin Kaung Myat , Takehara Sachiko , Lin Wa Than , Ogawa Hiroshi 
Published: January 2025

ABSTRACTBackgroundThe complex association between COVID‐19 and psychological factors may significantly impact oral health, including oral health‐related quality of life (OHRQoL). Despite their interconnected nature, literature regarding their collective impact on OHRQoL is limited.ObjectiveThis study aims to assess the independent and interactive effects of COVID‐19 infection and psychological factors on OHRQoL.Materials and MethodsThe cross‐sectional study included 158 participants from a health centre in Yangon city. Participants were categorised into three groups based on their COVID‐19 infection history, hospitalisation and oxygen supply: ‘not experienced’, ‘experienced, non‐hospitalised’ and ‘experienced, hospitalised’. Validated instruments, including Depression, Anxiety and Stress Scale‐21 (DASS‐21) to measure emotional states and the Oral Health Impact Profile‐14 (OHIP‐14) to assess OHRQoL, were employed. Descriptive statistics, Mann–Whitney U test, Kruskal–Wallis test, linear regressions and interaction analysis were computed.ResultsThe median OHIP‐14 score was 7.2. Among 85 confirmed COVID‐19 cases, 38.8% (n = 33) received hospitalisation and oxygen supply. Multivariable regression analysis identified ‘experienced, hospitalised’ (95% CI: 6.36, 11.76), difficulty falling asleep (95% CI: 2.65, 7.18), depressive symptoms (95% CI: 7.38, 12.44), anxiety symptoms (95% CI: 2.93, 7.52) and stress symptoms (95% CI: 4.55, 11.11) as predictors of OHIP‐14. Interaction analysis revealed their associations varied by hospitalisation history. In non‐hospitalised individuals, depressive symptoms were positively interacted with OHRQoL (95% CI: 1.34, 9.02). Significant associations were observed between ‘experienced, hospitalised’ × ‘depressive, anxiety and stress symptoms’ and OHRQoL in both univariable and multivariable analyses.ConclusionA COVID‐19 infection history and psychological distress significantly affect OHRQoL in Myanmar participants. Moreover, a strong interactive association was observed between hospitalisation during infection and symptoms of psychological distress.
Liver Disease Complicating Familial Mediterranean Fever: A Study on 66 Patients Out of 533 Adult From the JIR Cohort
Authors: Marion Delplanque , Xavier Amiot , Dominique Wendum , François Rodrigues , Aknouche Zohra , Bourguiba Rim , Benoit Terris , Christophe Duvoux , Pierre Bedossa , Didier Lebrec , Philippe Sogni , Lucia Parlati , Frederic Charlotte , Vlad Ratziu , Stéphane Mouly , Jeremy Augustin , Julien Calderaro , Giovanna Scoazec , Michel Vignaud Jean , Arnaud Seyrig Jacques , Gilles Grateau , Léa Savey , Sophie Georgin‐Lavialle 
Published: January 2025

ABSTRACTBackgroundFamilial Mediterranean fever (FMF) is the most common monogenic autoinflammatory disease, associated with MEFV mutations. FMF patients can experience liver involvement, potentially leading to cirrhosis.ObjectivesThis study aimed to evaluate liver involvement in FMF patients at a French tertiary centre for adult FMF.MethodsWe conducted an observational study with FMF patients displaying 2 pathogenic MEFV mutations at the National Reference Center for Autoinflammatory Diseases and Inflammatory Amyloidosis (CEREMAIA) in Paris and included in the JIR cohort. MEFV heterozygous patients and those with other liver disease causes were excluded.ResultsAmong 533 FMF patients 12.4% had chronic liver abnormalities, with 30% who developed cirrhosis 54 years [36–57] in median after disease onset. Forty‐seven per cent were colchicine resistant, and 41% received interleukin‐1 inhibitors. Cirrhotic patients experienced delayed hepatopathy diagnosis, prolonged FMF diagnosis delay and late‐onset treatment initiation compared to those with only liver function test abnormalities. Colchicine resistance and interleukin‐1 inhibitor use were more common in cirrhotic patients. Body mass index and AA amyloidosis rates did not differ significantly between groups. Twenty‐one patients had undergone liver biopsies including 14 cirrhotic patients revealing steatohepatitis in 12 cases and probable steatohepatitis in 4. Other lesions, like iron overload and sinusoidal dilatation, were sporadically observed.ConclusionFMF patients are at risk of chronic liver disease. Regular liver function monitoring is crucial, particularly in case of persistent inflammation, due to the risk of progression to cirrhosis and its associated morbidity and mortality.
The Autologous Fascial Pubovaginal Sling for Recurrent Stress Incontinence: A Retrospective Study
Authors: Luo Jin‐Yang , Shen Si‐Hong , Ye Zhen‐Yang , Xu Li‐Jing , Chen Jia‐Wei , Peng Liao , Shen Hong , Luo De‐Yi 
Published: January 2025

ABSTRACTAimThis study aimed to analyze the clinical efficacy and safety of autologous fascial pubovaginal sling (AFPVS) surgery in treating recurrent stress urinary incontinence (SUI) following the failure of mid‐urethral sling procedures.MethodsA retrospective analysis was conducted on the clinical data of SUI patients who underwent AFPVS at our hospital between 2008 June and 2024 June following the failure of mid‐urethral sling procedures. The analysis included basic information, surgical parameters, and postoperative complications. Additionally, telephone interviews using the International Consultation on Incontinence Questionnaire‐Short Form (ICI‐Q‐SF) and the Incontinence Quality of Life Questionnaire (I‐QOL) was conducted at 6‐month follow‐up.ResultsThirty‐four patients with the mean age of 54.8 ± 15.7 years old were reviewed, and the median disease duration was 3 (2–10) years. Of the 34 patients, 20 (58.8%) were cured, 8 (23.5%) showed improvement, resulting in an overall effectiveness rate of 82.3%. Three cases experienced bladder injury, which resolved after 2 weeks of catheterization, but no patients had urethral or vaginal injuries intraoperatively. Postoperative scores on the ICI‐Q‐SF significantly decreased (p < 0.001), and postoperative I‐QOL scores significantly increased (p < 0.001) compared to preoperative scores.ConclusionAFPVS is a safe and effective salvage procedure for recurrent SUI following the failure of mid‐urethral sling procedures.
Tip‐to‐base bark cross‐sectional areas contribute to understanding the drivers of carbon allocation to bark and the functional roles of bark tissues
Authors: Vázquez‐Segovia Karen , Olson Mark E. , Campo Julio , Ángeles Guillermo , Martínez‐Garza Cristina , Vetter Susanne , Rosell Julieta A. 
Published: January 2025

Summary
Along their lengths, stems experience different functional demands. Because bark and wood traits are usually studied at single points on stems, it remains unclear how carbon allocation changes along tip‐to‐base trajectories across species.
We examined bark vs wood allocation by measuring cross‐sectional areas of outer and inner bark (OB and IB), IB regions (secondary phloem, cortex, and phelloderm), and wood from stem tips to bases of 35 woody angiosperm species of diverse phylogenetic lineages, climates, fire regimes, and bark morphologies. We examined how varied bark vs wood allocation was and how it was affected by precipitation, temperature, soil fertility, leaf habit, and fire regime.
Allocation to phloem (relative to wood) varied little across species, whereas allocation to other tissues, strongly affected by the environment or shed in ontogeny, varied widely. Allocation to parenchyma‐rich cortex and phloem was higher at drier sites, suggesting storage. Higher allocation to phloem and cortex also occurred on infertile soils, and to phloem in drought‐deciduous vs cold‐deciduous and evergreen species. Allocation to OB was highest at sites with frequent fires and decreased with fire frequency.
Our approach contextualizes inferences from across‐species studies, allows testing functional hypotheses, and contributes to disentangling the functional roles of poorly understood bark tissues.
Validity and Utility of a Risk Prediction Model for Wound Infection After Lower Third Molar Surgery
Authors: Yamagami Akira , Narumi Katsuya , Saito Yoshitaka , Furugen Ayako , Imai Shungo , Okamoto Keisuke , Kitagawa Yoshimasa , Ohiro Yoichi , Takagi Ryo , Takekuma Yoh , Sugawara Mitsuru , Kobayashi Masaki 
Published: January 2025

ABSTRACTObjectivesTo externally validate a clinical prediction model for surgical site infection (SSI) after lower third molar (L3M) surgery and evaluate its clinical usefulness.MethodsWe conducted a retrospective cohort study of patients who underwent L3M surgery at Hokkaido University Hospital. The study was designed to evaluate the historical and methodological transportability. Clinical usefulness was evaluated using decision curve analysis on the data of the non‐antibiotic‐treated patients.ResultsWe obtained 2543 validation cohorts from April 2020 to March 2023, and 640 non‐antibiotic cohorts from July 2010 to September 2023. The incidences of SSI after L3M surgery were 5.3% (135/2543) and 7.7% (49/640) in the validation and non‐antibiotic cohorts, respectively. The discrimination ability of the prediction model was acceptable for the external validation cohort (c‐statistic: 0.67; 95% CI: 0.62–0.71) and adequate for the non‐antibiotic cohort (c‐statistic: 0.72; 95% CI: 0.63–0.79). In both cohorts, the model showed excellent calibration between the observed and predicted probabilities. Decision curve analysis showed increased net benefit across a range of meaningful risk thresholds.ConclusionA simple risk prediction model for SSI after L3M surgery demonstrated clinical transportability and usefulness. This model may help surgeons/clinicians determine the appropriateness of prophylactic antibiotics administration for patients in L3M surgery.
Simplified Jaw Functional Limitation Scale for Temporomandibular Disorders: Psychometric Evaluation
Authors: Sun Ruonan , Liu Tiqian , Liu Yiwei , Zhang Shuyuan , Yue Yuan , Jiang Nan , Zhang Liming , Xiong Xin 
Published: January 2025

ABSTRACTObjectiveThis study aimed to simplify the Jaw Functional Limitation Scale (JFLS) and evaluate its reliability and validity in assessing jaw function in patients with temporomandibular disorders (TMD) in primary care.MethodsTMD patients and non‐TMD participants were assessed using Diagnostic Criteria for Temporomandibular Disorders (DC/TMD). Demographic data and the 8‐item JFLS questionnaire were collected to quantify jaw functional restriction. Confirmatory factor analysis tested the validity of JFLS‐8 using chi‐square, comparative fit index (CFI), root mean square error of approximation (RMSEA), and root mean square residual (RMR). The study selected key items from each domain to create a 4‐item JFLS for further testing. Exploratory factor analysis, reliability, and validity tests were conducted.ResultsAnalysis included 671 valid questionnaires (502 TMD patients, 169 non‐TMD participants). JFLS‐8 exhibited satisfactory fit among TMD patients (RMSEA = 0.143, CFI = 0.881, RMR = 0.567). JFLS‐4 retained items with high factor loadings: JFLS1 (0.935), JFLS2 (0.923), JFLS7 (0.766), and JFLS5 (0.713). The simplified JFLS‐4 showed good reliability (Cronbach's alpha 0.714) and validity (Kaiser‐Meyer‐Olkin 0.558, Bartlett's test < 0.001) among TMD patients.ConclusionA 4‐item JFLS was developed and validated, demonstrating good reliability and validity in assessing mandibular function in TMD patients, exhibiting the potential to streamline evaluation processes in clinical practice.