bluuebunny/crossref_metadata_2025_split huggingface dataset:
Mechanism of ribosome-associated mRNA degradation during tubulin autoregulation
Authors: Höpfler Markus , Absmeier Eva , Peak-Chew Sew-Yeu , Vartholomaiou Evangelia , Passmore Lori A. , Gasic Ivana , Hegde Ramanujan S. 
Published: January 2023

SUMMARYMicrotubules play crucial roles in cellular architecture, intracellular transport, and mitosis. The availability of free tubulin subunits impacts polymerization dynamics and microtubule function. When cells sense excess free tubulin, they trigger degradation of the encoding mRNAs, which requires recognition of the nascent polypeptide by the tubulin-specific ribosome-binding factor TTC5. How TTC5 initiates decay of tubulin mRNAs is unknown. Here, our biochemical and structural analysis reveals that TTC5 recruits the poorly studied protein SCAPER to the ribosome. SCAPER in turn engages the CCR4-NOT deadenylase complex through its CNOT11 subunit to trigger tubulin mRNA decay. SCAPER mutants that cause intellectual disability and retinitis pigmentosa in humans are impaired in CCR4-NOT recruitment, tubulin mRNA degradation, and microtubule-dependent chromosome segregation. Our findings demonstrate how recognition of a nascent polypeptide on the ribosome is physically linked to mRNA decay factors via a relay of protein-protein interactions, providing a paradigm for specificity in cytoplasmic gene regulation.
Membrane-binding properties of NS1 proteins from Zika and Dengue viruses: Comparative simulations in explicit bilayers reveal significant differences
Authors: Muthukumaran Rajagopalan , Sankararamakrishnan Ramasubbu 
Published: January 2023

AbstractNS1 in flaviviruses is the only non-structural protein that is secretory and interacts with different cellular components. NS1 is localized in endoplasmic reticulum as a dimer to facilitate the viral replication. The crystal structures of NS1 homologs from zika (ZIKV) and dengue (DENV) viruses have revealed the organization of different domains in NS1 dimers. The β-roll and the connector and intertwined loop regions of wing domains of NS1 have been shown to interact with the membranes. The membrane-binding properties and the differences between ZIKV and DENV NS1 homologs in interacting with the membranes have not been investigated. In this study, we have performed molecular dynamics (MD) simulations of ZIKV and DENV NS1 systems in apo and in POPE bilayers with different cholesterol concentrations (0, 20 and 40%). In the simulations with bilayers, the NS1 protein was placed just above the membrane surface. At the end of 600 ns production runs, ZIKV NS1 inserts deeper inside the membrane compared to the DENV counterpart. The conformational landscape sampled by NS1 in the presence of membrane was analyzed. Unlike ZIKV NS1, the orientation of DENV NS1 is asymmetric in which one of the chains in dimer interacts with the membrane while the other is exposed to the solvent. The β-roll region in ZIKV NS1 penetrates beyond the headgroup region and some residues interact with the lipid acyl chains while the C-terminal region barely interacts with the headgroup. Specific residues in the intertwined region deeply penetrate inside the membrane with less interactions with water molecules. Our analysis showed that more charged residues of ZIKV NS1 are involved in stronger interactions with the headgroups than that found for DENV NS1. The role of hydrophobic and aromatic residues in interactions with acyl chain region is also evident. Presence of cholesterol affects the extent of insertion in the membrane and interaction of individual residues. This study clearly shows that the binding, insertion and interaction of ZIKV NS1 with the lipid bilayer significantly differs from its counterpart in DENV.
High frequency soil polarization can phenotype crop roots noninvasively
Authors: Gu Huijie , Cseresnyés Imre , Butnor John R. , Li Baoru , Mary Benjamin , Sun Hongyong , Zhang Xiying , Lu Yang , Liu Xiuwei 
Published: January 2023

AbstractNoninvasive and nondestructive root phenotyping techniques under field conditions are sorely needed to advance plant root science. Soil polarization measured by electrical capacitance (ECsoil) has the potential to meet this requirement, but whether it specifically detects root properties remains unexplored. We carried out manipulative experiments where wheat (Triticum aestivumL.) and maize (Zea maysL.) roots were buried in soil or immersed in hydroponic solution combined with pot trials to reveal the mechanism of root trait detection by ECsoil, while a field experiment was conducted to test its feasibility to determine root depth distribution. We found that ECsoilmeasured at low current frequency (< 1 kHz) was not significantly affected by the addition of roots to the system either by burying roots in soil or immersing them in solution. At frequency greater than10 kHz a shift occurred, and root polarization contributed more to ECsoilwhich was positively correlated with root volume. When ECsoilwas measured at high frequency (30 kHz −100 kHz) it was well correlated with root volume vertical distribution in the field. The measurement error after soil moisture calibration at depths of 10 cm, 20 cm, 30 cm and 40 cm was 0.4%, 12.0%, 1% and 34%, respectively. Our results demonstrate that ECsoilis a robust method to measurein situroot distribution and we believe the newly available high frequency measurement equipment combined with novel root prediction models will enable ECsoilto be widely used for root phenotyping in the future.
Pathogenesis of Breakthrough Infections with SARS-CoV-2 Variants in Syrian Hamsters
Authors: Plunkard Jessica , Mulka Kathleen , Zhou Ruifeng , Tarwater Patrick , Zhong William , Lowman Margaret , Wong Amanda , Pekosz Andrew , Villano Jason 
Published: January 2023

AbstractSevere acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the causative agent of COVID-19, has evolved into multiple variants. Animal models are important to understand variant pathogenesis, particularly for those with mutations that have significant phenotypic or epidemiological effects. Here, cohorts of naïve or previously infected Syrian hamsters (Mesocricetus auratus) were infected with variants to investigate viral pathogenesis and disease protection. Naïve hamsters infected with SARS-CoV-2 variants had consistent clinical outcomes, tissue viral titers, and pathology, while hamsters that recovered from initial infection and were reinfected demonstrated less severe clinical disease and lung pathology than their naïve counterparts. Males had more frequent clinical signs than females in most variant groups, but few sex variations in tissue viral titers and lung pathology were observed. These findings support the use of Syrian hamsters as a SARS-CoV-2 model and highlight the importance of considering sex differences when using this species.ImportanceWith the continued circulation and emergence of new severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) variants, understanding differences between the initial and a subsequent reinfection on disease pathogenesis is critical and highly relevant. This study characterizes Syrian hamsters as an animal model to study reinfection with SARS-CoV-2. Previous infection reduced the disease severity of reinfection with different SARS-CoV-2 variants.
Effectiveness of Paxlovid - a review
Authors: Paltra Sydney , Conrad Tim 
Published: January 2023

AbstractPaxlovid is an oral treatment for mild to moderate COVID-19 cases with a high risk for severe course of the disease. For this review, we have performed a comprehensive literature review. We present a summary of currently available data on Paxlovid’s ability to reduce the risk of progressing to a severe disease state. Our findings can be concluded as follows: data from the time when the Delta-variant was dominant shows that Paxlovid reduced the risk of hospitalization or death by 87.8% for unvaccinated, non-hospitalized high-risk individuals. Data from the time when the Omicron variant was dominant found decreased risk reductions, varying between 41% and 46%, combining various vaccination statuses. However, one study, which differentiated by age, found that the administration of Paxlovid reduced the risk of hospitalization by 67% for individuals aged 65 and older, but only by 27% for individuals aged 40-64. From the available data, one can conclude that Paxlovid cannot substitute vaccination, but its low manufacturing cost as well as its easy administration make it a valuable tool in fighting COVID-19, especially for countries with a low vaccination rate.
TGFB1 Induces Fetal Reprogramming and Enhances Intestinal Regeneration
Authors: Chen Lei , Dupre Abigail , Qiu Xia , Pellon-Cardenas Oscar , Walton Katherine D. , Wang Jianming , Perekatt Ansu O. , Hu Wenwei , Spence Jason R. , Verzi Michael P. 
Published: January 2023

SUMMARYThe adult gut epithelium has a remarkable ability to recover from damage. To achieve cellular therapies aimed at restoring and/or replacing defective gastrointestinal tissue, it is important to understand the natural mechanisms of tissue regeneration. We employed a combination of high throughput sequencing approaches, mouse genetic models, and murine and human organoid models, and identified a role for TGFB signaling during intestinal regeneration following injury. At 2 days following irradiation (IR)-induced damage of intestinal crypts, a surge in TGFB1 expression is mediated by monocyte/macrophage cells at the location of damage. Depletion of macrophages or genetic disruption of TGFB-signaling significantly impaired the regenerative response following irradiation. Murine intestinal regeneration is also characterized by a process where a fetal transcriptional signature is induced during repair. In organoid culture, TGFB1-treatment was necessary and sufficient to induce a transcriptomic shift to the fetal-like/regenerative state. The regenerative response was enhanced by the function of mesenchymal cells, which are also primed for regeneration by TGFB1. Mechanistically, integration of ATAC-seq, scRNA-seq, and ChIP-seq suggest that a regenerative YAP-SOX9 transcriptional circuit is activated in epithelium exposed to TGFB1. Finally, pre-treatment with TGFB1 enhanced the ability of primary epithelial cultures to engraft into damaged murine colon, suggesting promise for the application of the TGFB-induced regenerative circuit in cellular therapy.GRAPHIC ABSTRACT
IL-17 undermines longevity and stress tolerance by inhibiting a protective transcriptional network
Authors: Lu Qiongxuan , Vladareanu Ioana , Zhao Lina , Nilsson Lars , Henriksson Johan , Chen Changchun 
Published: January 2023

AbstractAberrant cytokine secretion contributes to the pathogenesis of autoimmune diseases and age-related disorders, but the molecular mechanism underlying this is not entirely clear. Here, we elucidate how interleukin-17 (IL-17) overactivation shortens lifespan and damages defense mechanisms against stress inC. elegans. Our analysis reveals that NHR-49, theC. elegansortholog of human PPARα and HNF4, is the central component in the transcriptional network undermined by increased IL-17 signaling. Both NHR-49 and its coactivator MDT-15 physically interact with the downstream components of IL-17 pathway, and their expression is significantly decreased when IL-17 signaling is enhanced. IL-17 overactivation also induces the expression and nucleus entry of theC. elegansortholog of NF-κB inhibitor NFKI-1/IκBζ to repress the activity of transcriptional coactivator MDT-15 and CBP-1. IL-17 signaling acts on neurons to modulate the activity of NFKI-1/IκBζ and NHR-49. In addition, persistent IL-17 activation decreases the expression of HLH-30/TFEB, leading to the reduced transcription of lysosomal lipase genes in the distal tissues. All these jointly contribute to the increased sensitivity to oxidative stress of animals with enhanced IL-17 signaling. Collectively, our work illustrates a transcription system undermined by IL-17 overactivation in the animals without NF-κB, and provides mechanistic insight into the pathogenesis of abnormal IL-17 secretion.
Investigating the influence of total productive maintenance key success factors on the social sustainability dimension of manufacturing SMEs
Authors: Samadhiya Ashutosh , Agrawal Rajat , Garza-Reyes Jose Arturo 
Published: January 2023

PurposeKey success factors (KSFs) of total productive maintenance (TPM) have historically played a vital role in attaining economic and ecological sustainability but have overlooked social sustainability. Hence, this study analyses and ranks the most significant TPM KSFs for attaining social sustainability in manufacturing small and medium enterprises (SMEs).Design/methodology/approachThe research employs a deductive methodology to identify the relevant TPM KSFs and social sustainability indicators and then uses Fuzzy Technique for Order of Preference by Similarity to Ideal Solution (TOPSIS) to rank the TPM KSFs in order to achieve social sustainability, followed by a sensitivity analysis to assess the methodological robustness.FindingsThe findings indicate that the top five TPM KSFs influencing social sustainability are employee health and safety, organizational culture, top management commitment, employee engagement and effective communication and effective workplace management. In addition, the results indicate that effective equipment utilization is the least significant TPM key factor affecting social sustainability.Research limitations/implicationsSME manufacturing managers do not need to worry about all of the TPM KSFs if they only concentrate on the ones that will have the most impact. If managers use the top 5 TPM KSFs as a starting point, they may create customized TPM training programs for their companies. As a result, this will facilitate the efforts of their personnel toward social sustainability.Originality/valueIn the existing literature, little emphasis has been paid to social sustainability and how SMEs may implement these practices. This research adds to the current theory of TPM and social sustainability and sheds light on how SMEs might use TPM to advance toward more socially sustainable operations.
Integrating distribution, sales and services in manufacturing: a comparative case study
Authors: Andersen Torben Juul , Bering Søren 
Published: January 2023

PurposeThe aim of this study is to gain important insights on integration oriented servitization identifying essential dimensions of effective structures, coordination approaches and management controls adopted by manufacturing firms that integrate forward towards distribution, sales and services.Design/methodology/approachThe study adopts a theory-guided qualitative abductive methodology to conduct a comparative case-study of two manufacturing firms in the same industry integrating forward to enhance servitization but with significantly different performance outcomes. The findings are uncovered from a broad spectrum of primary and secondary data spanning two decades.FindingsThe consistently high-performing firm puts equal emphasis on production and downstream distribution, sales and services and motivate individuals to engage in entrepreneurial efforts to develop combined product-services offerings that are valued by customers. The underperforming firm prioritizes operating efficiency driven by engineering prowess and managed through planning, standardization, authority and central controls.Research limitations/implicationsThe study is based on two representative firms operating in a specific industry context, which has ramifications for the generalizability of results and calls for replication studies to substantiate and extend findings.Practical implicationsForward integration from manufacturing into distribution, sales and services represents a specific servitization strategy that needs structure and particular coordination approaches to be effective in complex dynamic product-markets. The characteristics of the outperforming case company provide useful insights on effective integrated servitization efforts.Social implicationsForward integration is a commonly adopted strategy among manufacturing firms that constitute the backbone of modern economies and effective governance of these integration oriented servitization efforts has important implications for societal value creation.Originality/valueThis study builds on rationales from management science including economic theory, corporate strategy and different micro-foundational lenses and thereby hone recent calls for broader theoretical foundations to enlighten studies of the servitization puzzle.
Customer involvement and servitization in hybrid offerings: moderating role of digitalization and co-creation
Authors: Behl Abhishek , Kamboj Shampy , Sarmah Bijoylaxmi , Pereira Vijay , Sharma Kirti , Rammal Hussain Gulzar , Arrigo Elisa 
Published: January 2023

PurposeThis study examines the impact of customer involvement (CI), technology strategy, firm internationalization and servitization on product and service innovation performance (SIP) in hybrid offerings. In addition, it investigates the moderating role of digitization and co-creation in the relationship mentioned above.Design/methodology/approachA research framework was developed through the lens of service-dominant (S-D) logic theory, and the proposed research hypotheses were empirically tested. Primary data were collected via the survey method, and structural equation modeling was used to analyze the data.FindingsFindings of this study suggest that the S-D logic theory effectively explains CI and servitization in hybrid offerings. Furthermore, digitization is a crucial driver of SIP. Additionally, this paper finds that co-creation moderates between servitization and innovation performance of hybrid offerings.Practical implicationsBesides theoretical contributions, this study presents valuable insights to manage service networks during servitization.Originality/valueFirst, this work proposes a comprehensive framework of hybrid offerings' driving factors (i.e. CI, firm internationalization, technology strategy and servitization) and their impact on product and SIP. Second, it tests the moderating effects of digitalization and co-creation in the context of hybrid offerings.
Institutional isomorphism under the test of Non-financial Reporting Directive. Evidence from Italy and Spain
Authors: Posadas Stefanía Carolina , Ruiz-Blanco Silvia , Fernandez-Feijoo Belen , Tarquinio Lara 
Published: January 2023

Purpose
This paper aims to analyse the impact of the European Union (EU) Directive on the quality of sustainability reporting under the institutional theory lens. Specifically, the authors evaluate what kind of institutional pressure has the highest impact on the quality of corporate disclosure on sustainability issues.

Design/methodology/approach
The authors build a quality index based on the content analysis of sustainability information disclosed, before and after the transposition of the Directive, by Italian and Spanish companies belonging to different industries. The authors use an OLS regression model to analyse the effect of coercive, normative and mimetic forces on the quality of the sustainability reports.

Findings
The results highlight that normative and mimetic mechanisms positively affect the quality of sustainability reporting, whereas there is no evidence regarding coercive mechanisms, indicating that the new requirements do not provide a significant contribution to the development of better reporting practices, at least in the two analysed countries.

Originality/value
To the best of the authors’ knowledge, this is one of the few studies assessing the quality of sustainability reporting through an analysis involving the period before and after the implementation of the EU Directive. It enriches the literature on institutional theory by analysing how the different dimensions of isomorphism affect the quality of information disclosed by companies according to the EU requirements. It contributes to a better understanding of the impact of the non-financial information Directive, and the results of this paper can be relevant for regulators, practitioners and academia, especially in view of the adoption of the new Corporate Sustainability Reporting Directive proposal.
Facile ligand-exchange strategy to promote low-temperature nano-sintering of oleylamine-capped Ag nanoparticles
Authors: Li Liyun , Zhang Yu , Xia Shiyu , Sun Zhefei , Yuan Junjie , Su Dongchuan , Cao Hunjun , Chai Xiaoming , Wang Qingtian , Li Jintang , Zhang Zhihao 
Published: January 2023

Purpose
This study aims to develop a facile ligand-exchange strategy to promote nano-sintering of oleylamine (OAM)-capped silver nanoparticles (AgNPs). By using ligand exchange process with NH4OH to remove OAM from the surface of AgNP, this study reports effectively reducing the sintering temperature of AgNPs to achieve low-temperature nano-sintering. Compared with untreated AgNPs of OAM-capped, NH4OH-treated AgNPs possess superior sintering performance that could be applied to a fractional generator device as conductor and in favour of the fabrication of flexible circuit modules.

Design/methodology/approach
First, oleylamine is used as reductant to synthesize monodisperse AgNPs by a simple one-step method. Then ligand exchange is used with NH4OH at different treating times to remove OAM, and micro-Fourier transform infrared spectroscopy and contact angle test are applied to clear the mechanism and structure characteristics of these processes. Finally, NH4OH-treated AgNPs sediment sintering is used at different temperatures to test electrical resistivity and use ex situ scanning electron microscopy combined with in situ X-ray diffraction to study changes in microstructure in the whole nano-sintering process.

Findings
The AgNPs are always capped by organic ligands to prevent nanoparticles agglomeration. And oleylamine used as reductant could synthesize desirable size distributions of 8–32 nm with monodisperse globular shapes, but the low-temperature nano-sintering seemed not to be achieved by the oleylamine-capped AgNPs because OAM is an organic with long C-chain. The ligand exchange approach was enabled to replace the original organic ligands capped on AgNPs with organic ligands of low thermal stability which could promote nano-sintering. After ligand exchange treated AgNPs could be sintered on photo paper, polydimethylsiloxane (PDMS) and polyethylene terephthalate flexible substrates at low temperature.

Originality/value
In this research, the method ligand exchange is used to change the ligand of AgNPs. During ligand exchange, NH4OH was used to treat AgNPs. Through the treatment of NH4OH, the change of hydrophilic and hydrophobic properties of AgNPs was successfully realized. The sintering temperature of AgNPs can also be reduced and the properties can be improved. Finally, the applicability of the AgNPs sediment with this nano-sintering process at low temperature for obtaining conductive patterns was evaluated using PDMS as substrates.