bluuebunny/crossref_metadata_2025_split huggingface dataset:
Dynamic SARS-CoV-2-specific B-cell and T-cell responses induced in people living with HIV after a full course of inactivated SARS-CoV-2 vaccine
Authors: Wang Xiuwen , Yang Xiaodong , Zhang Xin , Yan Hongxia , Jin Junyan , Ma Zhenglai , Duan Junyi , Zhang Guanghui , Huang Tao , Li Yongzheng , Wu Hao , Zhang Tong , Zhu Aiwei , Jin Cong , Song Xiangrong , Su Bin 
Published: February 2025

ObjectiveBoth B-cell- and T-cell-mediated immunity are crucial for the effective clearance of viral infection, but little is known about the dynamic characteristics of SARS-CoV-2-specific B-cell and T-cell responses in people living with HIV (PLWH) after a full course of inactivated SARS-CoV-2 vaccination.MethodsIn this study, fifty people living with HIV (PLWH) and thirty healthy controls (HCs) were enrolled to assess B-cell and T-cell responses at the day before the vaccination (T0), two weeks after the first dose (T1), two months after the first dose (T2), the day of the third dose (T3), one month after the third dose (T4), three months after the third dose (T5) and 12 months (T6) after the third dose.ResultsSARS-CoV-2-specific B-cell and T-cell responses were induced in people living with HIV (PLWH), and these responses lasted at least one year after the third vaccine dose. However, the peak frequencies of Spike-specific B-cell and T-cell responses in PLWH were lower than those in HIV-negative controls. In addition, the expansion of activated B cells, memory B cells and plasma cells after primary vaccination was observed, but the percentages of these cells were decreased at T6 and were comparable to those at T0. Additionally, the percentages of activated T cells, exhausted T cells and SARS-CoV-2-specific T cells with enhanced functional activity were increased following the administration of inactivated SARS-CoV-2 vaccine. In addition, PLWH had lower percentages of plasma cells, RBD-specific B cells, circulating Tfh (cTfh) cells and CD38+ cTfh cells, and the percentages of the latter two types of cells were positively correlated with the titer of neutralizing antibodies, indicating these differences may account for the weaker immune responses induced in PLWH.ConclusionThese data suggest that specific B-cell and T-cell responses could be sustained for at least one year after receiving the third vaccination. Our findings emphasize that the weak SARS-CoV-2-specific B-cell and T-cell responses induced in PLWH have implications for clinical decision-making and public health policy for PLWH with respect to SARS-CoV-2 infection.
Big data analysis and machine learning of the role of cuproptosis-related long non-coding RNAs (CuLncs) in the prognosis and immune landscape of ovarian cancer
Authors: Kuang Mingqin , Liu Yueyang , Chen Hongxi , Chen Guandi , Gao Tian , You Keli 
Published: February 2025

BackgroundOvarian cancer (OC) is a severe malignant tumor with a significant threat to women’s health, characterized by a high mortality rate and poor prognosis despite conventional treatments such as cytoreductive surgery and platinum-based chemotherapy. Cuproptosis, a novel form of cell death triggered by copper ion accumulation, has shown potential in cancer therapy, particularly through the involvement of CuLncs. This study aims to identify risk signatures associated with CuLncs in OC, construct a prognostic model, and explore potential therapeutic drugs and the impact of CuLncs on OC cell behavior.MethodsWe analyzed ovarian cancer data (TCGA-OV) from the TCGA database, including transcriptomic and clinical data from 376 patients. Using Pearson correlation and LASSO regression, we identified 8 prognostic CuLncs to construct a risk signature model. Patients were categorized into high- and low-risk groups based on their risk scores. We performed survival analysis, model validation, drug sensitivity analysis, and in vitro experiments to assess the model’s performance and the functional impact of key CuLncs on OC cell proliferation, invasion, and migration.ResultsThe prognostic model demonstrated significant predictive power, with an area under the curve (AUC) of 0.702 for 1-year, 0.640 for 3-year, and 0.618 for 5-year survival, outperforming clinical pathological features such as stage and grade. High-risk OC patients exhibited higher Tumor Immune Dysfunction and Exclusion (TIDE) scores, indicating stronger immune evasion ability. Drugs such as JQ12, PD-0325901, and sorafenib showed reduced IC50 values in the high-risk group, suggesting potential therapeutic benefits. In vitro experiments revealed that knockdown of LINC01956, a key CuLnc in the risk signature, significantly inhibited the proliferation, invasion, and migration of OC cells (P&lt;0.05).ConclusionOur study identified a prognostic risk model based on CuLncs and explored their potential as therapeutic targets in OC. The findings highlight the importance of CuLncs in OC prognosis and immune response, providing new insights for future research and clinical applications.
Malaria case management over 15 years (2006–2021) in Jakiri North-West Region Cameroon
Authors: Ndang Ndong Henry , Mengnjo Njodzela Christian , Netongo Palmer Masumbe , Soniran Olajoju Temidayo , Chu Chuo Ennestine , Chiabi Eugene , Amambua-Ngwa Alfred , Cheng Ndong Ignatius 
Published: February 2025

BackgroundMalaria remains a serious health challenge; thus, the need for continual improvements in treatment regimens and the adoption of new treatment guidelines to enhance case management is vital. We report trends in malaria case management over 15 years at the Jakiri Health Centre in Cameroon, an area that has been plagued with civil unrest since 2016.MethodsData were collected retrospectively from consultation, laboratory, antenatal care (ANC) and prescription registers, and double entered into Excel. Analysis was conducted using SPSS statistics and Microsoft Excel.ResultsA total of 3,800 febrile patients visited the outpatient department (OPD), and patients received 8,324 prescriptions. Of these, 11.6% (964/8324) were anti-malarials, 3.5% (291/8324) were ferrous sulfate (FS), 40.8% (3,396/8324) were antibiotics (AB), and 44.1% (3,673/8324) were analgesics. The antimalarials prescribed were artemisinin-based combination therapies (ACTs) 83.2% (802/964) and quinine (Q) 16.8% (162/964). No ACTs were prescribed between 2006 and 2011. The 5 to &lt;15 years age group received the most proportion of ACTs 42.3% (189/447), followed by the 1 to &lt;5 years age group 31.8% (127/399). Males were more likely to be prescribed ACTs than females were (OR= 1.336, 95% CI 1.141–1.564; p&lt;0.0001). Between 2006 and 2013, the number of quinine prescriptions increased from 17.2% (21/122) in 2006 to a peak of 26.1% (30/115) in 2009. Antibiotic prescription rates were high across all age groups. The prescription of antimalarials to malaria-negative patients was relatively low over time. ACTs were prescribed to all participants attending the ANC who were confirmed malaria-positive. 97.7% of SP was prescribed as intermittent preventive treatment in pregnancy (IPTp) with highest prescription rates observed among women in their third trimester 96.2% (76/79).ConclusionThis study demonstrated that recommended malaria treatment guidelines have been adopted by the Jakiri Health Centre, which has transitioned from quinine as a first-line treatment to ACTs. ACTs were not used in the facility until 2012. Though anti-malaria prescription was largely appropriate, antibiotics and analgesics were highly over prescribed. There is great need for continuous monitoring and refresher training for health workers to enforce adherence to the guidelines.
Nomograms of risk prediction and prognosis for the T1-T2 stage gastric cancer with lymph node metastasis: a population-based study
Authors: Nie Guo-Le , Geng Longlong , Zhang Hao , Chu Shicheng , Jiang Hong 
Published: February 2025

Background and aimsLymph node metastasis plays a crucial role in determining the appropriate treatment approach for patients with gastric cancer (GC), particularly those in the T1–T2 stage. Currently available diagnostic strategies for GC with lymph nodes have limited accuracy. The present research aimed to create and validate diagnostic and prognostic nomograms specifically tailored for the T1–T2 stage GC patients with LNM.MethodsWe derived clinicopathological characteristics of patients diagnosed with GC from the Surveillance, Epidemiology, and End Results (SEER) database. We utilized univariate and multivariate logistic analyses to examine the risk factors linked with the occurrence of lymph node metastasis (LNM) in GC patients within the T1–T2 stage. Furthermore, the prognostic factors related to the T1–T2 stage GC patients with LNM were explored by univariate and multivariate cox analyses. Two nomograms were built by the risk factors screened above.ResultsUltimately, our study included 5,350 patients with T1–T2 stage GC. After identifying age, T stage, tumor size, primary site, grade, and histological type as risk factors for the LNM occurrence, we successfully developed a diagnostic nomogram utilizing these variables. Age, T stage, M stage, tumor size, primary site, grade, radiation, surgery, and chemotherapy were all independent prognostic factors that related to the T1 – T2 GC patients with LNM. The results of the AUC, calibration curve and decision curve analysis (DCA) showed excellent calibration performance and clinical applicability of the two nomograms. The Kaplan–Meier (K-M) curves clearly demonstrated a notable distinction in overall survival between low-risk and high-risk groups, highlighting the prognostic significance of the nomogram.ConclusionThe establishment and validation of the two nomograms for T1-T2 GC patients with LNM were successful, serving as valuable tools for clinical decision-making and the formulation of personalized treatment approaches.
Effect of acupuncture in patients with postprandial distress syndrome: study protocol for a randomized controlled trial
Authors: Yan Zhaobo , Xu Xuan , Liu Mailan , MuRong Zhimiao , Zhong Huan , Luo Rong , He Haolong , Liu Weiai , Du Geshu , Liu Mi 
Published: February 2025

BackgroundPostprandial distress syndrome (PDS) is the prominent subtype in patients with functional dyspepsia (FD) and currently lacks a satisfactory treatment. Acupuncture has become a promising alternative and complementary therapy for managing FD. However, high-level clinical evidence supporting the use of acupuncture for FD is limited.MethodsThis study is a multicentre, double-dummy, single-blind, randomized, active-controlled trial. Two hundred and one eligible participants will be randomly assigned into three groups: a verum acupuncture plus placebo group, an itopride plus sham acupuncture group, and a sham acupuncture plus placebo group. This study consists of a 1-week screening period, a 4-week treatment period, and a 12-week follow-up period. During the intervention period, participants will receive 12 sessions of verum or sham acupuncture treatment (one session per day, three sessions per week, for 4 weeks) along with 50 mg itopride tablets or 50 mg itopride placebo tablets 3 times a day for 20 days (5 continual days a week for 4 weeks). The response rate (patients who had adequate relief of gastric symptoms will be considered positive responders) and the elimination rate of cardinal symptoms (postprandial fullness and early satiation) are the primary indicators to evaluate the overall acupuncture effect for PDS. Secondary outcome measures will include the Nepean Dyspepsia Symptom Index (NDSI), the short form-Nepean Dyspepsia Life Quality Index (SF-NDLQI), the Hospital Anxiety and Depression Scale (HADS), and related hormone concentrations. Participants’ expectations toward acupuncture treatment will also be assessed, and adverse events will be recorded for safety assessment. All analyses will adhere to an intention-to-treat principle.DiscussionIn conclusion, this trial will determine the efficacy and safety of acupuncture for PDS and provide more high-level evidence to support its application in treating FD.Trial registrationIdentifier [ITMCTR2024000510].
Perceived value and barriers of nursing specialty certifications among clinical nurses in Saudi Arabia: a cross-sectional study
Authors: AlSadah Alawiah T. , Aboshaiqah Ahmad E. , Alanazi Naif H. 
Published: February 2025

IntroductionSpecialty nursing certifications reflect nurse’s knowledge and competence in certain areas. Obtaining certification allows them to advance their careers and enhance patient care standards as their role and scope of responsibility expands. This study aimed to understand how nurses view specialty certification and related challenges in three university hospitals in Riyadh, Saudi Arabia.MethodsA cross-sectional, correlational design was used. A survey questionnaire (Perceived Value of Certification-12 Tool) was sent through email, and it had one question for each barrier.ResultsThe majority of participants valued certification to verify specialist knowledge (93.9%), enhance achievement (82.8%), and increase competence (79.1%). Certification was associated extrinsically with hiring preference (54.7%), recognition from colleagues (52.3%), and professional autonomy (51.7%). The primary barriers were lack of institutional reward (72.7%) and examination cost (64.8%), with not passing earlier being the least mentioned barrier (2%). The biggest obstacles faced by clinical nurses in obtaining certification include limited institutional rewards because their organization did offer incentives, such as promotions or salary bumps. Age and hospital significantly predicted clinical nurses’ intrinsic value of certification, while age, gender, nationality, and hospital significantly predicted their extrinsic value. Age and gender significantly influenced their overall perceived value of certification.DiscussionOverall, nurses in Riyadh recognize the value of certification. However, focused interventions must be implemented to overcome identified challenges and amplify the professional benefits of certification. Recommended measures include implementing tailored support initiatives and financial aid programs to address these barriers. A collaborative approach should be developed to provide support and actively promote the pursuit of nursing certification. Future studies should adopt longitudinal and qualitative designs to evaluate the impact of focused strategies.
Whole-genome sequencing-based species classification, multilocus sequence typing, and antibiotic resistance mechanisms of the clinical Aeromonas complex
Authors: Lu Junwan , Zhang Lei , Yan Chunxia , Lin Naru , Zhang Yuan , Sha Yuning , Zhao Jingxuan , Lu Jun , Bao Qiyu , Zhang Guozhi 
Published: February 2025

BackgroundMultidrug-resistant strains of the genus Aeromonas can produce various β-lactamases that confer resistance to a broad spectrum of β-lactams, which poses a significant public health threat due to their emergence and spread in clinical settings and natural environments. Therefore, a comprehensive investigation into the antibiotic resistance mechanisms of Aeromonas is scientifically significant.MethodsBetween 2018 and 2021, 78 clinical Aeromonas isolates were collected from human clinical specimens. The MicroScan WalkAway system and average nucleotide identity (ANI) analyses were used to classify the bacterial species. Antibiotic susceptibility was determined through the minimum inhibitory concentration (MIC) test via the agar dilution method. To determine the resistance mechanism and the structure of the resistance gene-related sequences, molecular cloning, whole-genome sequencing and bioinformatic analysis were performed.ResultsAmong the 78 Aeromonas isolates studied in this work, obtained from various specimens from different clinical departments, 77 were classified into seven known species by ANI analysis. Most of the isolates were A. caviae (34.6%, 27/78), followed by A. hydrophila (25.6%, 20/78). Multilocus sequence typing (MLST) revealed that they belonged to 72 sequence types (STs), including 52 new STs. A total of 334 resistance genes of 30 antibiotic resistance genotypes were identified from the genomes, more than half (55.99%, 187/334) of which were β-lactamase genes. The isolates showed much higher rates of resistance to penicillins (penicillin G, 98.7%) and first-generation cephalosporins (cefazolin, 96.2%), but lower resistance rates to fourth-generation cephalosporins (cefepime, 6.4%), monobactams (aztreonam, 5.1%), and carbapenems (imipenem, 1.3% and meropenem, 5.1%). Structural analyses of some β-lactamase genes (such as blaNDM-1 and blaPER-3) related sequences revealed that they were generally associated with mobile genetic elements.ConclusionThe investigation of the correlation between the distribution of β-lactamase genes and Aeromonas resistance phenotypes in this study suggested an urgent need for rigorous monitoring and control to counteract the escalating public health threat posed by the increase in Aeromonas strains harboring extended-spectrum β-lactamase and metallo-β-lactamase genes.
Imaging markers of cerebral small vessel disease are associated with Alzheimer’s disease: a systematic review and meta-analysis
Authors: Wu Qi , Zhang Jupeng , Lei Peng , Zhu Xiqi , Huang Changhui 
Published: February 2025

ObjectiveThis study aims to assess the correlation between cerebral small vessel disease (CSVD) imaging markers and the risk of Alzheimer’s disease (AD) through a systematic review and meta-analysis.MethodsIn July 2024, two researchers independently conducted a thorough literature search across databases such as PubMed, Embase, Web of Science, and the Cochrane Library. The selected studies investigated the correlations between white matter hyperintensities (WMHs), cerebral microbleeds (CMBs), lacunar infarction (LI), and enlarged perivascular spaces (EPVS) and the risk of AD. The Newcastle-Ottawa Scale (NOS) was employed to assess the risk of bias in the included cohort and case–control studies.ResultsA total of 6,487 articles were identified, with 15 articles meeting the inclusion criteria. Pooled analyses showed that WMHs (HR: 1.38, 95% CI: 1.10–1.74, N = 7,661), CMBs (HR: 1.60, 95% CI: 1.07–2.40, N = 6,567), and EPVS (HR: 1.84, 95% CI: 1.24–2.72, N = 3,045) were associated with an increased risk of AD, with EPVS showing the strongest correlation. LI did not show a statistically significant association with an increased risk of AD (HR: 1.41, 95% CI: 0.98–2.01, N = 4,014).ConclusionWMHs, CMBs, and EPVS are associated with an elevated risk of AD, whereas LI is considered a potential risk factor. However, additional studies are required to determine the role of CSVD markers in AD progression.
A new method for identifying and evaluating depressive disorders in young people based on cognitive neurocomputing: an exploratory study
Authors: Liu Jiakang , Li Kai , Li Shuwu , Liu Shangjun , Wang Chen , Huang Shouqiang , Tu Yuting , Wang Bo , Zhang Pengpeng , Luo Yuntian , Sun Guanqun , Chen Tong 
Published: February 2025

BackgroundDepressive disorders are one of the most common mental disorders among young people. However, there is still a lack of objective means to identify and evaluate young people with depressive disorders quickly. Cognitive impairment is one of the core characteristics of depressive disorders, which is of great value in the identification and evaluation of young people with depressive disorders.MethodsThis study proposes a new method for identifying and evaluating depressive disorders in young people based on cognitive neurocomputing. The method evaluates cognitive impairments such as reduced attention, executive dysfunction, and slowed information processing speed that may exist in the youth depressive disorder population through an independently designed digital evaluation paradigm. It also mines digital biomarkers that can effectively identify these cognitive impairments. A total of 50 young patients with depressive disorders and 47 healthy controls were included in this study to validate the method’s identification and evaluation capability.ResultsThe differences analysis results showed that the digital biomarkers of cognitive function on attention, executive function, and information processing speed extracted in this study were significantly different between young depressive disorder patients and healthy controls. Through stepwise regression analysis, four digital biomarkers of cognitive function were finally screened. The area under the curve for them to jointly distinguish patients with depressive disorders from healthy controls was 0.927.ConclusionThis new method rapidly characterizes and quantifies cognitive impairment in young people with depressive disorders. It provides a new way for organizations, such as schools, to quickly identify and evaluate the population of young people with depressive disorders based on human-computer interaction.
Integrated multi-biomarker panel of CXCL13, HS-CRP, and WBC counts predicts outcomes in stroke neurosyphilis patients treated with HBO and TUS-NMES
Authors: He Wenchao , Chen Shuangshuang , Chen Ruyang , Zhang Jun , Zhang Xuehua , Wu Minzhi , Zhang Dan , Zhu Fengfeng , He Fanghua , Xv Yating , Lei Na , Zheng Wenhui , Shan Xinyi , Dai Jun 
Published: February 2025

BackgroundNeurosyphilis results from Treponema pallidum invading the central nervous system, leading to severe neurological issues like stroke. Combining hyperbaric oxygen (HBO) therapy and transcranial ultrasound neuromuscular stimulation (TUS-NMES) shows promise in improving outcomes.ObjectiveThis study evaluates the predictive value and clinical significance of CXCL13, WBC, and Hs-CRP levels in neurosyphilis patients undergoing HBO and TUS-NMES therapy.MethodsThe study included 158 neurosyphilis stroke patients treated from June 2022 to January 2024. Assessments of limb motor, cognitive functions, daily living abilities, and cerebrospinal fluid biomarkers were conducted pre- and post-four weeks of combined therapy.ResultsAfter treatment, there was a significant improvement in FMA, MoCA, and MBI scores (p &lt; 0.001). CXCL13 levels significantly decreased post-treatment, correlating with improved patient outcomes. The study found strong predictive values for CXCL13 levels in determining the efficacy of rehabilitation, with the combination of CXCL13, WBC, and Hs-CRP showing the highest predictive accuracy.ConclusionHBO and TUS-NMES significantly enhance recovery in neurosyphilis stroke patients. CXCL13, WBC, and Hs-CRP effectively predict rehabilitation outcomes, highlighting their value in clinical management.
Retrobulbar/intraconal tube placement in patients with glaucoma: Ahmed FP-7 and tube extender case series with 1 year follow-up
Authors: Laroche Daniel , Grodecki Brian , Grimes Kara Rickford , Ng Chester 
Published: February 2025

PurposeThe Ahmed FP-7 valve is useful in the management of refractory glaucoma. However, this can often have ocular hypertensive phase and subconjunctival fibrosis that can lead to increased medication use and failure. We report how retrobulbar and intraconal plate placement with tube extension can avoid the ocular hypertensive phase, lower intraocular pressure, and reduce medication burden.Patients and methodsThis is a retrospective case series of 4 patients with glaucoma who underwent Ahmed FP-7 valve and retrobulbar/intraconal tube placement with a one-year follow-up.ResultsOne-year results in 4 patients with advanced glaucoma and pseudophakia revealed a pre-operative intraocular pressure of 21 mmHg on 5.5 medications. At one year, the post-operative intraocular pressure was 10.25 on 0 medications. The vision and visual fields were stable. One patient required drainage of a choroidal effusion and one patient required burping of viscoelastic on post-operative day one.ConclusionsThe combined insertion of the Ahmed FP-7 valve and silicone tube inserted into the retrobulbar/intraconal space has been shown to prevent ocular hypertensive phase, lower intraocular pressure, and reduce medication burden at one year.