bluuebunny/crossref_metadata_2025_split huggingface dataset:
Suppression of tomato wilt by cell-free supernatants of Acinetobacter baumannii isolates from wild cacao from the Colombian Amazon
Authors: Pisco-Ortiz Carolina , González-Almario Adriana , Uribe-Gutiérrez Liz , Soto-Suárez Mauricio , Amaya-Gómez Carol V. 
Published: September 2023

Abstract
Tomato vascular wilt caused by Fusarium oxysporum f. sp. lycopersici (Fol) is one of the most limiting diseases of this crop. The use of fungicides and varieties resistant to the pathogen has not provided adequate control of the disease. In this study, siderophore-producing bacteria isolated from wild cocoa trees from the Colombian Amazon were characterized to identify prominent strategies for plant protection. The isolates were taxonomically classified into five different genera. Eight of the fourteen were identified as bacteria of the Acinetobacter baumannii complex. Isolates CBIO024, CBIO086, CBIO117, CBIO123, and CBIO159 belonging to this complex showed the highest efficiency in siderophore synthesis, producing these molecules in a range of 91–129 µmol/L deferoxamine mesylate equivalents. A reduction in disease severity of up to 45% was obtained when plants were pretreated with CBIO117 siderophore-rich cell-free supernatant (SodSid). Regarding the mechanism of action that caused antagonistic activity against Fol, it was found that plants infected only with Fol and plants pretreated with SodSid CBIO117 and infected with Fol showed higher levels of PR1 and ERF1 gene expression than control plants. In contrast, MYC2 gene expression was not induced by the SodSid CBIO117 application. However, it was upregulated in plants infected with Fol and plants pretreated with SodSid CBIO117 and infected with the pathogen. In addition to the disease suppression exerted by SodSid CBIO117, the results suggest that the mechanism underlying this effect is related to an induction of systemic defense through the salicylic acid, ethylene, and priming defense via the jasmonic acid pathway.

Graphical abstract
Towards facing uncertainties in biofuel supply chain networks: a systematic literature review
Authors: Habibi Farhad , Chakrabortty Ripon K. , Abbasi Alireza 
Published: September 2023

AbstractBiofuel supply chains (BSCs) face diverse uncertainties that pose serious challenges. This has led to an expanding body of research focused on studying these challenges. Hence, there is a growing need for a comprehensive review that summarizes the current studies, identifies their limitations, and provides essential advancements to support scholars in the field. To overcome these limitations, this research aims to provide insights into managing uncertainties in BSCs. The review utilizes the Systematic Reviews and Meta-Analyses (PRISMA) method, identifying 205 papers for analysis. This study encompasses three key tasks: first, it analyses the general information of the shortlisted papers. Second, it discusses existing methodologies and their limitations in addressing uncertainties. Lastly, it identifies critical research gaps and potential future directions. One notable gap involves the underutilization of machine learning techniques, which show potential for risk identification, resilient planning, demand prediction, and parameter estimations in BSCs but have received limited attention. Another area for investigation is the potential of agent-based simulation, which can contribute to analysing resilient policies, evaluating resilience, predicting parameters, and assessing the impact of emerging technologies on BSC resilience in the twenty-first century. Additionally, the study identifies the omission of various realistic assumptions, such as backward flow, lateral transshipments, and ripple effects in BSC. This study highlights the complexity of managing uncertainties in BSCs and emphasizes the need for further research and attention. It contributes to policymakers’ understanding of uncertain sources and suitable approaches while inspiring researchers to address limitations and generate breakthrough ideas in managing BSC uncertainties.
The Grounds of Excuses
Authors: van Loon Marie 
Published: September 2023

AbstractAccording to a popular view, excuses undermine blameworthiness. At the same time, philosophers commonly accept that blameworthiness is composed of two necessary conditions: a moral objectionability condition and a responsibility condition. For excuses to do their job, they must undermine at least one of these conditions. In this paper, I conclude that excuses do neither. By inference to the best explanation, I propose a view that reconciles this conclusion with the function of excuses.
Plasmonics: An Optical Approach to Study Plant Epigenetics
Authors: Mamián-López Mónica Benicia , Begcy Kevin 
Published: September 2023

AbstractEssential factors that control gene stability and expression are collectively known as epigenetics. Within the most well-studied epigenetic mechanisms are DNA methylation and histone modifications. A broad range of methods has been used for identifying differentially methylated regions, including biotechnological and enzymatic techniques. Nevertheless, in the last decade, there has been a proliferation of techniques called plasmonics which have emerged as an alternative to studying epigenetics. They take advantage of the different chemical composition of methylated compared to unmethylated histones and nucleotides to quantify their optical properties. Here, we introduce the basics of plasmonics and present a detailed description of how these techniques work. We also provide an outlook on the application of plasmonics in plant epigenetics.
Local signal variability and functional connectivity: Sensitive measures of the excitation-inhibition ratio?
Authors: van Nifterick Anne M. , Scheijbeler Elliz P. , Gouw Alida A. , de Haan Willem , Stam Cornelis J. 
Published: September 2023

AbstractA novel network version of permutation entropy, the inverted joint permutation entropy (JPEinv), holds potential as non-invasive biomarker of abnormal excitation-inhibition (E-I) ratio in Alzheimer’s disease (AD). In this computational modelling study, we test the hypotheses that this metric, and related measures of signal variability and functional connectivity, are sensitive to altered E-I ratios. The E-I ratio in each neural mass of a whole-brain computational network model was systematically varied. We evaluated whether JPEinv, local signal variability (by permutation entropy) and functional connectivity (by weighted symbolic mutual information (wsMI)) were related to E-I ratio, on whole-brain and regional level. The hub disruption index can identify regions primarily affected in terms of functional connectivity strength (or: degree) by the altered E-I ratios. Analyses were performed for a range of coupling strengths, filter and time-delay settings. On whole-brain level, higher E-I ratios were associated with higher functional connectivity (by JPEinv and wsMI) and lower local signal variability. These relationships were nonlinear and depended on the coupling strength, filter and time-delay settings. On regional level, hub-like regions showed a selective decrease in functional degree (by JPEinv and wsMI) upon a lower E-I ratio, and non-hub-like regions showed a selective increase in degree upon a higher E-I ratio. These results suggest that abnormal functional connectivity and signal variability, as previously reported in patients across the AD continuum, can inform us about altered E-I ratios.
Zur Bedeutsamkeit der Unterrichtsqualität für die Entwicklung von Rechtschreibkompetenzen in der Grundschule
Authors: Jaeuthe Jessica , Lenkeit Jenny , Bosse Stefanie , Spörer Nadine 
Published: September 2023

ZusammenfassungDie Entwicklung der Rechtschreibkompetenz wird in theoretischen Modellen mit Hilfe von aufeinander aufbauenden Kompetenzniveaus beschrieben, in denen die Schülerinnen und Schüler zu Beginn nicht lautgetreu, dann lautgetreu und zuletzt orthographisch korrekt schreiben. Diese Entwicklung kann theoretisch durch zahlreiche Merkmale beeinflusst werden. Dabei mangelt es an empirischen Studien, die Merkmale der Unterrichtsqualität berücksichtigen. Hier setzt die vorliegende Studie an, in der die Rechtschreibkompetenz der Schülerinnen und Schüler zu drei Messzeitpunkten (Anfang Klasse 2, Ende Klasse 2, Ende Klasse 3) erhoben wurde, um mit Hilfe von latenten Transitionsanalysen die Rechtschreibkompetenz drei Profilen zuzuordnen. Anschließend wurden die Einschätzungen der Schülerinnen und Schüler zur Unterrichtsqualität in multinomialen logistischen Mehrebenen-Regressionsmodellen analysiert. Es zeigte sich, dass die Unterrichtsqualität die Rechtschreibkompetenzentwicklung nicht wie vermutet vorhersagen konnte. So zeigte sich zwar ein signifikanter Befund für den Zusammenhang der Rechtschreibkompetenz mit der kognitiven Aktivierung, jedoch fiel dieser entgegen der Erwartung negativ aus. Es wird diskutiert, inwiefern dies durch die methodische Anlage der Studie bedingt sein könnte und welche methodischen Hinweise dies für zukünftige Studien liefert.
Multimorbidity in Heart Failure: Leveraging Cluster Analysis to Guide Tailored Treatment Strategies
Authors: van de Veerdonk Mariëlle C. , Savarese Gianluigi , Handoko M. Louis , Beulens Joline W.J. , Asselbergs Folkert , Uijl Alicia 
Published: September 2023

Abstract
Review Purpose
This review summarises key findings on treatment effects within phenotypical clusters of patients with heart failure (HF), making a distinction between patients with preserved ejection fraction (HFpEF) and reduced ejection fraction (HFrEF).

Findings
Treatment response differed among clusters; ACE inhibitors were beneficial in all HFrEF phenotypes, while only some studies show similar beneficial prognostic effects in HFpEF patients. Beta-blockers had favourable effects in all HFrEF patients but not in HFpEF phenotypes and tended to worsen prognosis in older, cardiorenal patients. Mineralocorticoid receptor antagonists had more favourable prognostic effects in young, obese males and metabolic HFpEF patients. While a phenotype-guided approach is a promising solution for individualised treatment strategies, there are several aspects that still require improvements before such an approach could be implemented in clinical practice.

Summary
Stronger evidence from clinical trials and real-world data may assist in establishing a phenotype-guided treatment approach for patient with HF in the future.
Effect of Dielectric Barrier Discharge Cold Plasma on the Bio-nanocomposite Film and its Potential to Preserve the Quality of Strawberry under Modified Atmosphere Packaging
Authors: Rashvand Mahdi , Matera Attilio , Altieri Giuseppe , Genovese Francesco , Nikzadfar Mehrad , Feyissa Aberham Hailu , Di Renzo Giovanni Carlo 
Published: September 2023

AbstractThe present study aimed to investigate the effect of cold plasma on the developed bio-nanocomposite film. A dielectric barrier discharge system was utilized to generate the cold plasma. The films were treated under three different times (5, 10 and 15 min) and the characteristics of the films were evaluated. The cold plasma treatment influence the thermal stability and crystallization of the films. Results showed that the mechanical properties (tensile strength and elongation at break), water vapor permeability, oxygen transmission rate, moisture content and water contact angle characteristics were improved up to 69%, 31%, 34%, 3% and 28%, respectively by cold plasma treatment. In consequence, the average O2 and CO2 concentration of the packed strawberries decreased from 5 and 10% to 4.2% and 5.1%, respectively after 15 days. Finally, the results revealed that the mechanical properties, chemical attributes, physical characteristics and microbial activities of the samples were affected by the treated films. As a result, cold plasma modification can be applied as an effective method to maintain and preservation of fresh fruit.
Predicting the thermal performance of screen mesh wick heat pipe with alumina nanofluids using response surface methodology
Authors: Lakshmi Reddy P. , Sreenivasa Reddy B. , Govindarajulu K. , Bandhu Din , Saxena Ashish 
Published: September 2023

AbstractThis study aims to investigate the effect of heat load, tilt angle, and volume concentration of alumina nanofluid on the thermal performance enhancement of cylindrical screen mesh wick heat pipe in terms of thermal resistance(R) and thermal conductivity(k). Response surface methodology based on the Box–Behnken design was implemented to investigate the influence of heat input (100–200 W), tilt angle (0–90°), and volume concentration (0.05–0.25 vol%) of nanofluid as the independent variables. Second-order polynomial equations were established to predict the responses, ‘R’, and ‘k’. The significance of the models was tested by means of analysis of variance (ANOVA). In addition, a correlation between the independent variables was derived in this study. The results revealed that the optimum heat input, inclination angle, and concentration of nanofluid were determined as 200 W, 52.72°, and 0.1773 vol. % respectively for both ‘R’ and ‘k’. SEM analysis was performed to observe the thermal performance phenomena of the heat pipe before and after experimentation.
Zinc Deficiency as a General Feature of Cancer: a Review of the Literature
Authors: Sugimoto Rie , Lee Lingaku , Tanaka Yuki , Morita Yusuke , Hijioka Masayuki , Hisano Terumasa , Furukawa Masayuki 
Published: September 2023

AbstractTrace elements are minerals that are present in very low concentrations in the human body and yet are crucial for a wide range of physiological functions. Zinc, the second most abundant trace element, is obtained primarily from the diet. After being taken up in the intestine, zinc is distributed to various target organs, where it plays key roles in processes such as immunity, protein folding, apoptosis, and antioxidant activity. Given the important role of zinc in a wide range of enzymatic reactions and physiological processes, zinc deficiency has been identified in a variety of diseases, notably cancer. In recent years, multiple meta-analyses and reviews looking at zinc levels in individual cancer types have been published, as have a plethora of primary studies demonstrating a link between low zinc levels and specific types of cancer. In this review, we summarize recent evidence implicating low zinc concentrations in serum or tissues as a characteristic in a wide range of cancers. We also discuss preliminary findings indicating that zinc level measurement could ultimately become a useful clinical tool for cancer diagnosis and predicting outcomes in patients with cancer. Finally, we suggest future directions for further elucidating the role of zinc deficiency in cancer development and progression.
Inhibited Expression of NLRP12 Promotes the Development of Triple-Negative Breast Cancer by Activating the NF-κB Pathway
Authors: Kuang Wenbin , Gu Qingdan , Zhou Ying , Xiao Xiaoqin , He Dabao , Deng Qiuchan 
Published: September 2023

AbstractNLRP12 can affect the progression of different diseases, including hepatocellular carcinoma. However, no report on triple-negative breast cancer (TNBC) has been found. Thus, this study aimed to explore the role of NLRP12 in TNBC. In our study, immunohistochemistry, real-time quantitative PCR (qPCR), and Western blot assays were used to evaluate NLRP12 expression in TNBC tissues and cells. Then, NLRP12 lentivirus was constructed and infected into MDA-MB-231 and MDA-MB-157 cells with or without PTD-p65-P1 treatment. Next, cells were collected for cell function detection using the following procedures: colony formation assay for proliferation, Transwell for migration and invasion, and Western blot for NF-κB and MAPK pathway-associated proteins. Finally, a xenograft mouse model was applied; the tumor volume and weight were determined, and NLRP12, p-IκBb-α, and p-IκBb-α expressions were evaluated using qPCR and Western blot. Results indicated that NLRP12 was lowly expressed in TNBC tissues and cells. The inhibition of NLRP12 could induce the proliferation, migration, and invasion of TNBC cells, which also could be reversed by inhibiting the NF-κB pathway (PTD-p65-P1). Moreover, silencing of NLRP12 could upregulate p-IκBb-α, while IκBb-α, p-ERK, ERK, p-p38, p38, p-JNK, and JNK expressions remained unchanged, thereby indicating that only the NF-κB pathway could be activated by NLRP12 silencing. Furthermore, the xenograft mouse model confirmed the abovementioned findings. Therefore, the low expression of NLRP12 promoted the proliferation, migration, and invasion in TNBC cells by activating the NF-κB pathway. This study might provide insights into TNBC therapy.
Clinical Correlation of Altered Molecular Signatures in Epileptic Human Hippocampus and Amygdala
Authors: Modarres Mousavi Sayed Mostafa , Alipour Fatemeh , Noorbakhsh Farshid , Jafarian Maryam , Ghadipasha Masoud , Gharehdaghi Jaber , Kellinghaus Christoph , Speckmann Erwin-Josef , Stummer Walter , Khaleghi Ghadiri Maryam , Gorji Ali 
Published: September 2023

AbstractWidespread alterations in the expression of various genes could contribute to the pathogenesis of epilepsy. The expression levels of various genes, including major inhibitory and excitatory receptors, ion channels, cell type-specific markers, and excitatory amino acid transporters, were assessed and compared between the human epileptic hippocampus and amygdala, and findings from autopsy controls. Moreover, the potential correlation between molecular alterations in epileptic brain tissues and the clinical characteristics of patients undergoing epilepsy surgery was evaluated. Our findings revealed significant and complex changes in the expression of several key regulatory genes in both the hippocampus and amygdala of patients with intractable epilepsy. The expression changes in various genes differed considerably between the epileptic hippocampus and amygdala. Different correlation patterns were observed between changes in gene expression and clinical characteristics, depending on whether the patients were considered as a whole or were subdivided. Altered molecular signatures in different groups of epileptic patients, defined within a given category, could be viewed as diagnostic biomarkers. Distinct patterns of molecular changes that distinguish these groups from each other appear to be associated with epilepsy-specific functional consequences.
Alisol A Exerts Neuroprotective Effects Against HFD-Induced Pathological Brain Aging via the SIRT3-NF-κB/MAPK Pathway
Authors: Lu Taotao , Ding Linlin , Zheng Xiaoqing , Li Yongxu , Wei Wei , Liu Weilin , Tao Jing , Xue Xiehua 
Published: September 2023

AbstractChronic consumption of a high-fat diet (HFD) has profound effects on brain aging, which is mainly characterized by cognitive decline, inflammatory responses, and neurovascular damage. Alisol A (AA) is a triterpenoid with therapeutic potential for metabolic diseases, but whether it has a neuroprotective effect against brain aging caused by a HFD has not been investigated. Six-month-old male C57BL6/J mice were exposed to a HFD with or without AA treatment for 12 weeks. Behavioral tasks were used to assess the cognitive abilities of the mice. Neuroinflammation and changes in neurovascular structure in the brains were examined. We further assessed the mechanism by which AA exerts neuroprotective effects against HFD-induced pathological brain aging in vitro and in vivo. Behavioral tests showed that cognitive function was improved in AA-treated animals. AA treatment reduced microglia activation and inflammatory cytokine release induced by a HFD. Furthermore, AA treatment increased the number of hippocampal neurons, the density of dendritic spines, and the expression of tight junction proteins. We also demonstrated that AA attenuated microglial activation by targeting the SIRT3-NF-κB/MAPK pathway and ameliorated microglial activation-induced tight junction degeneration in endothelial cells and apoptosis in hippocampal neurons. The results of this study show that AA may be a promising agent for the treatment of HFD-induced brain aging.
Protective and vulnerability factors of posttraumatic growth during the COVID-19 pandemic
Authors: Noriega Cristina , Sánchez-Cabaco A. , López J. , Pérez-Rojo G. , Sitges E. , Bonete-López B. 
Published: September 2023

AbstractBackground and objectives: The COVID-19 pandemic is a major life event that has brought new challenges and threats. Some people may experience positive changes, such as post-traumatic growth (PTG). Several factors may enhance or hinder this possibility of development. There is extensive literature considering protective and vulnerability factors separately. However, there is a lack of studies considering both factors based on a holistic approach. This study aims to determine the weight of two protective factors (resilience and life purpose) and two vulnerability ones (anxiety and depression) in PTG, considering the COVID-19 affectation. Design and Methods: 749 Spanish people aged 18–84 years old completed a survey that included sociodemographic data, direct/indirect affectation by COVID-19, anxiety, depression, resilience, life purpose and PTG. Path analysis was used to test the predictors of PTG. Results: Women showed higher levels of PTG. The effect of COVID-19 symptoms on PTG is mainly mediated by life purpose, and anxiety to a lesser extent. Two indirect effects were found: (1) the negative effect of depression on PTG through resilience and life purpose assessed simultaneously; (2) the positive effect of resilience on PTG through life purpose. Conclusions:The originality of the approach of this research lies in the integration of vulnerability and protection factors to determine PTG. Identifying protective and vulnerability factors is crucial to prevent the development of mental disorders in risk populations. The development of interventions considering depressive disorders as PTG inhibitors while highlighting life purpose to strengthen mental health is needed.
Network analysis of negative emotion and self-management in Chinese patients with early chronic kidney disease
Authors: Cui Yi , Guo Zhihua , Yang Tianqi , Liu Qin , Liu Na , Yang Haoshuang , Zhang Lihua , Mu Hezi , Zhang Lanfang , Zhang Yinling 
Published: September 2023

AbstractThe number of patients with chronic kidney disease (CKD) is increasing worldwide, and the disease carries a serious physical and psychological burden that creates negative emotions among patients. The negative emotions limits patients’ ability to manage their disease and prevents them from effectively delaying disease progression. In this study, we applied a network analysis to explore the network relationship between negative emotions and self-management in patients with CKD and to investigate the risk or protective effects of different components of negative emotions on self-management. The study was conducted from September 2021 to March 2022 in three tertiary hospitals in China, with data gathered via a convenience sampling method. 360 patients with CKD at stages 1 ~ 3 in the department of nephrology completed the Sociodemographic Questionnaire, Positive Affect and Negative Affect Scale and Chronic Kidney Disease Self-management Instrument. We used R4.1.1 software to estimate the network model and calculate the related indicators. The network showed that among negative emotions “irritated” was negatively correlated with “self-integration”, “problem solving”, “seeking social support” and “adherence to recommended regimen” in self-management. The correlations between “scared” and “self-integration”, “nervous” and “problem solving”, and “ashamed” and “seeking social support” were all positive. The bridge expected influence of “irritated” and “adherence to recommended regimen” were the highest, with values of -0.19 and 0.13, respectively. “Irritated” has a more obvious risk effect on self-management. This study provides an important target for interventions to reduce negative emotions and improve self-management ability in patients with CKD.