bluuebunny/crossref_metadata_2025_split huggingface dataset:
The genetic landscape of copy number variation in a Vietnamese cohort of 5008 fetuses with clinical anomalies during pregnancy
Authors: Tran Danh-Cuong , Dao Hong-Thuy Thi , Nguyen Hong-Dang Luu , Nguyen Duy-Anh , Le Quang Thanh , Hoang Diem-Tuyet Thi , Tran Nhat Thang , Ha Thi Minh Thi , Dinh Thuy Linh , Nguyen Canh Chuong , Doan Kim Phuong Thi , Luong Lan Anh Thi , Vo Ta Son , Trinh Thu Huong Nhat , Nguyen Van Thong , Vo Phuong-Anh Ngoc , Nguyen Yen-Nhi , Dinh My-An , Phan Minh Ngoc , Doan Phuoc-Loc , Do Thanh-Thuy Thi , Nguyen Quynh-Tho Thi , Truong Dinh-Kiet , Nguyen Hoai-Nghia , Phan Minh-Duy , Tang Hung-Sang , Giang Hoa 
Published: January 2023

Abstract
Copy number variation (CNV) analysis is a powerful tool for discovering structural genomic variation. Still, no program uses this tool to analyze chromosomal aneuploidies in the Vietnamese population. Pregnant women attending routine prenatal checkups in Vietnam from October 2018 to May 2021 were included in this study and contributed fetal tissue to test the utility of CNV analysis for prenatal screening. Among 5,008 women screened, 958 (19.13%) harbored at least one CNV, comprising segmental aneuploidy (8.49%), trisomy (6.91%), multiple anomalies (2.10%), and sex chromosome abnormality (1.64%). The rate of segmental aneuploidy detection increased with gestational age, but trisomy and sex chromosomal abnormalities detection decreased as the pregnancy continued. This study also found an association between abnormal CNVs and several phenotypic markers. For ultrasound soft markers, an increased nuchal fold thickness correlated with a higher risk of abnormal CNVs. In addition, many soft indicators or structural abnormalities were significantly associated with an increased likelihood of abnormal CNVs. This work highlights the importance of CNV analysis for the early detection of prenatal congenital abnormalities, especially in the first trimester. This study’s findings will meaningfully aid policymakers in developing cost-effective genetic prenatal screening programs.
Cross-species neuroimaging intermediate phenotypes deepen our understanding of depression
Authors: Guo Huiling , Dong Shuai , Xiao Yao , Yang Jingyu , Zhao Pengfei , Zhao Tongtong , Cai Aoling , Wang Hui , Hua Ruifang , Liu Rongxun , Wei Yange , Sun Dandan , Liu Zhongchun , Xia Mingrui , He Yong , Wu Yankun , Si Tianmei , Womer Fay Y , Xu Fuqiang , Wang Jie , Zhang Weixiong , Zhang Xizhe , Wang Fei 
Published: January 2023

Abstract
Multiple genetic variants and their interplay with environmental factors have hindered the progress of mental disease research and the development of effective markers of neuropsychiatric disorders. Intermediate phenotypes like neuroimaging brain patterns offer unique opportunities to understand multifaceted etiologies of neuropsychiatric diseases such as depression. Neuroimaging intermediate phenotypes bridging etiologic differences and disease behavioral features may facilitate translational applications of animal models to humans with depression. We identified cross-species neuroimaging patterns of the amplitude of low-frequency fluctuations (ALFF) that correlated with anhedonia in rodent genetic and stress models of depression and depressed individuals. Compared to controls, converse ALFF patterns in subcortical and sensorimotor regions were found between P11 knockout mice and chronic unpredictable mild stress rats. Similarly, two ALFF subtypes with converse patterns in frontal, subcortical, and sensorimotor regions were identified and validated in two independent human cohorts for depression. Importantly, anhedonia was significantly increased across all rodent models and human subtypes when compared to controls, despite differences in ALFF patterns. Further, anhedonia correlated with subcortical-sensorimotor ALFF in rodent models and human cohorts. Thus, subcortical-sensorimotor ALFF may serve as an intermediate phenotype that bridges etiologic differences and anhedonia in depression. These results deepened our knowledge of disease mechanisms underlying depression which can be explored in translational research and clinical applications treating depression and other psychiatric disorders.
Novel strategy to enhance the biological treatment of coal chemical wastewater by nano-zero valent iron loaded fly ash-based activated carbon assisted activated sludge process
Authors: Ma Weiwei , Zhang Xiaoqi , Han Hongjun , Shi Xueqing , Kong Qiaoping , Yu Tong , Zhao Fei 
Published: January 2023

Abstract
The study was to explore the performance of nanoscale zero valent iron loaded fly ash-based activated carbon (nZVI@FABAC) enhanced activated sludge (AS) process for the removal of toxic organic compounds in coal chemical wastewater (CCW). The results demonstrated that the removal efficiencies of COD and TPh in nZVI@FABAC/AS process reached about 83.96% and 85.17%, respectively, which were significantly increased compared with the single AS process. And the acute toxic units value of CCW was effectively reduced by 88.24% after nZVI@FBAC/AS process treatment. Microbial community analysis revealed that nZVI@FBAC might stimulate the AS system to enrich the various functional bacteria including phenol degrading bacteria (Comamonas and Acinetobacter), electroactive bacteria (Geobacter) and iron reduction bacteria (Geothrix), which provided various electron transfer pathways and facilitated the interspecies cooperation of different bacteria for improving degradation of toxic organic compounds in CCW. From the engineering, economic, environmental perspective, the nZVI@FBAC/AS process has a promising application for industrial wastewater treatment.
Left parathyroid carcinoma with secondary hyperparathyroidism: a case report
Authors: Yokoyama Ko , Suganuma Nobuyasu , Rino Yasushi 
Published: January 2023

Abstract
Background: Parathyroid carcinoma is a rare disease with a frequency of 0.005% of all malignancies [1,2]. Furthermore, cases with secondary hyperparathyroidism are fewer. In this case report, we describe a case of left parathyroid carcinoma with secondary hyperparathyroidism.
Case presentation: The patient was a 54-year-old woman that had been on hemodialysis since the age of 40 years. At 53 years of age, her calcium levels were high, and she was diagnosed with drug-resistant secondary hyperparathyroidism and was referred to our hospital for surgical treatment. Blood tests revealed calcium levels of 11.4 mg/dL and intact parathyroid hormone (PTH) levels of 1007 pg/mL. Neck ultrasonography revealed a 22-mm large round hypoechoic mass, partially indistinct margins, and D/W ratio > 1 at the left thyroid lobe. Computed tomography (CT) scans revealed a 20-mm nodule at the left thyroid lobe. No enlarged lymph nodes or distant metastasis were noted. 99mTc-hexakis-2-methoxyisobutylisonitrile (MIBI) scintigraphy revealed an accumulation at the superior pole of the left thyroid lobe. Laryngeal endoscope revealed paralysis of the left vocal cord, signifying recurrent nerve palsy due to parathyroid carcinoma. Based on these results, a diagnosis of secondary hyperparathyroidism and suspected left parathyroid carcinoma was made, and the patient underwent surgery. Pathology results revealed hyperplasia in the right upper and lower parathyroid glands in addition to capsular and venous invasion in the left upper parathyroid gland. The diagnosis was left parathyroid carcinoma. At 5 months post-surgery, calcium levels improved to 10.3 mg/dL and intact PTH levels to 9 pg/mL, with no signs of recurrence.
Conclusions: we report a case of left parathyroid carcinoma associated with secondary hyperparathyroidism. Concomitant secondary hyperparathyroidism may cause mild hypercalcemia compared to parathyroid carcinoma alone due to the added modification of dialysis. Although our patient also presented with mild hypercalcemia, a D/W ratio > 1 on preoperative echocardiography and presence of recurrent nerve palsy on laryngoscopy led to the suspicion and treatment of parathyroid carcinoma preoperatively.
Synthesis, Solvatochromism and Fluorescence Quenching Studies of Naphthalene Diimide Dye by Nanographene Oxide
Authors: Mahdiani Mojgan , Rouhani Shohre , Zahedi Payam 
Published: January 2023

Abstract
A naphthalene diimide dye with two side amine arm was prepared. Uv-Vis and fluorescence spectroscopic techniques studied its photophysical and solvatochromic characteristics in different solvents. Its fluorescence quenching mechanism has been carried out as a fluorescence probe model with graphene oxide as an efficient quencher. The results indicated that graphene plays an effective quencher against the naphthalene diimide molecule. The Stern-Volmer analysis results show a combination of two static and dynamic quenching mechanisms via an energy transfer process.
Light and heavy chain deposition disease with focal amyloid deposition diagnosed with mass spectrometry: A case report
Authors: Shimamoto Yuki , Takahashi Naoki , Katoh Nagaaki , Matsui Yuki , Mochizuki Yusuke , Ito Masanori , Yazaki Masahide , Kametani Fuyuki , Kasuno Kenji , Sekijima Yoshiki , Naiki Hironobu , Iwano Masayuki 
Published: January 2023

Abstract
Background

Light and heavy chain deposition disease (LHCDD) is a rare condition characterised by deposition of immunoglobulin components in the kidneys. Amyloidosis is also caused by the deposition of immunoglobulins, which are folded into amyloid fibrils characterised by Congophilic deposits that exhibit apple-green birefringence under polarised light. Reports of LHCDD with amyloid fibril deposition have been published, but details, such as the components of the deposits, have not been described.

Case presentation

We report the case of a 79-year-old Japanese woman who presented with nephrotic syndrome. Bone marrow examination revealed monoclonal gammopathy of unknown significance. Renal biopsy revealed amyloid-like deposits in the glomerulus that were positive for IgA and kappa on immunofluorescence. Congo red staining of the deposits was faintly positive, and only slight birefringence was detected. Electron microscopy revealed fine fibrillar structures and non-amyloid deposits. Mass spectrometry revealed abundant amounts of light chains with small amounts of heavy chains. Hence, the patient was diagnosed with LHCDD and focal amyloid deposition. Chemotherapy was initiated, which resulted in mild response. Under polarised light, faint birefringence with Congo red staining and periodic acid-methenamine silver positivity indicated that the deposits were mostly non-amyloid fibrils with amyloid fibrils. Heavy- and light-chain amyloidosis is diagnosed in cases wherein heavy chain deposition is greater than light chain deposition. However, in the present case, light-chain deposition was greater than heavy-chain deposition, which is not consistent with the definition of heavy- and light-chain amyloidosis.

Conclusion

This is the first case of LHCDD with focal amyloid deposition that was diagnosed by analysis of glomerular deposits using mass spectrometry.
Sex chromosome complement and organizational hormonal effects on the vasopressinergic sexual dimorphic pressor and antidiuretic responses
Authors: Lihue Gonzalez , Dadam Florencia María , Vivas Laura , Godino Andrea , Caeiro Ximena Elizabeth 
Published: January 2023

Abstract

The present study aimed to analyze the role of the sex chromosomes (SCC:XX/XY) and the interaction with the organizational effects of gonadal steroids on the vasopressinergic sexually dimorphic antidiuretic and pressor responses. For this purpose, we used transgenic mice of the "four core genotypes" model, in which the effect of gonadal sex and SCC are dissociated. To study vasopressin dimorphism in the antidiuretic response, mice were gonadectomized at 60 days old, and six weeks later were subcutaneously injected with desmopressin (V2R agonist, 1 mg/kg) and vehicle solution (isotonic saline solution). Four hour later urine samples were obtained for urinary osmolality determination. In a different group of animals, kidneys were excised for Avpr2 mRNA evaluation by qPCR. We also evaluated changes in blood pressure and heart rate in anesthetized mice in response to a 30-minute vasopressin (0.2 UI/ml, infusion volume 100µl) continuous infusion. As expected, desmopressin administration induced a significant effect of treatment {F(1,37) = 439,63}, however no SCC nor organizational hormonal effects were observed in absence of the activational hormonal effects. Furthermore, no differences in Avpr2 mRNA levels were found. However, the statistical analysis of the percentage changes in mean arterial pressure due to continuous acute vasopressin infusion demonstrated an interaction between the organizational hormonal effect and SCC {F(6,144) = 3,891, p = 0,0012}. Male mice with XX-SCC background showed a greater pressor response than the other genotypes. Our data may contribute to the understanding in hydromineral and blood pressure regulation of the complex interplay between vasopressin and SCC/organizational hormonal backgrounds.
Comparison of Outcomes of Subsequent Delivery Among Previous Vacuum and Previous Second Stage Cesarean Section Delivery Mothers at Kawempe National Referral Hospital: A Retrospective Cohort Study
Authors: Kamwesigye Assen , Kayiga Herbert , Beyeza-Kashesya Jolly , Nolens Barbara , Muriuki Moses 
Published: January 2023

Abstract
Introduction: The increasing trends in cesarean section (CS) with a decrease in operative vaginal deliveries is a major concern in health care system all over the world, particularly in low-resource settings. Studies show that a first delivery by CS is associated with an increased risk of repeat CS in the subsequent deliveries. In addition, CS compared to operative vaginal delivery (OVD), attracts higher health service costs yet resource-constrained countries have low rates of OVD compared to high-income countries. The aim of this study was to compare outcomes of the subsequent delivery among previous vacuum and previous second stage cesarean section delivery mothers at Kawempe National Referral Hospital.
Methods: This was a retrospective cohort study that involved physical or phone interviews of 81 mothers who had an operative delivery in their index pregnancy at Mulago hospital between November 2014 to July 2015. Maternal and neonatal outcomes in the subsequent delivery were compared among participants who had a previous vacuum extraction with those that previouslyhad a second stage cesarean section using Chi-2 square test and a Fisher’s exact test with a 0.05 level of statistical significance. A multivariate logistic regression was performed to determine association between mode of index delivery and maternal and neonatal outcomes in the subsequent delivery.
Results: Higher rates of vaginal delivery were achieved among women who had a vacuum extraction (78.4%) compared to those that had a second stage cesarean section in their index delivery (38.6%), p-value =< 0.001. The risk of neonatal injuries was higher in the group of women with a previous second stage cesarean delivery compared to those with a previous vacuum extraction (11.4% compared to 0%, p value of 0.034).
Conclusions and recommendations: Vacuum extraction does not only provide a safe and effective way to prevent the increasing rates of primary cesarean section, but also reduces the risk of repeat cesarean section and the complications associated with it. Health workers need to continue to offer choice of vacuum extraction in the second stage of labor among prime gravidas that fulfill its indication. This will help curb the up-surging rates in cesarean section.
Scansys Versus Pentacam HR: Evaluating the Possible Interchangeability in Healthy Subjects
Authors: khodaparast Mehdi , Hashemian Hesam , Mohmmed Marrwan Hisham , Ahmadzadeh Homan , Hamedani Majid Akhzari , Khorrami-Nejad Masoud 
Published: January 2023

Abstract

Purpose To determine the interchangeability of Scansys (MediWorks, Shanghai, China) and Pentacam HR (Oculus, Wetzlar, Germany) in healthy participants. Methods Eighty-nine eyes of 45 normal individuals underwent three consecutive scans of Scansys and Pentacam HR. Anterior segment measurements included anterior and posterior K-reading, central corneal thickness (CCT), thinnest corneal thickness (TCT), pupil center pachymetry, corneal volume, chamber volume, anterior chamber depth (ACD), anterior chamber angle (ACA), intraocular pressure (IOP), sphericity (Q-value), pupil diameter, and horizontal white-to-white (HWTW). Infraclass correlations (ICC) for reliability and Bland-Altman plots for the agreement between the mean findings of each device were analyzed. Results The mean age of participants was 31.5 ± 7.3 (range, 19–49) years (male/female = 21/24). Most anterior segment parameters had excellent or good reliability in ICC analysis. However, ACA and HWTW were two parameters with moderate reliability. Analysis of Bland-Altman plots showed that the bias line for all measurements was close to zero, except for posterior flat-k and steep-k readings; however, the discrepancy was not large enough to be clinically important. Analysis of the limit of agreement in Bland-Altman plots also displayed narrow limits and clinically irrelevant differences in 95% LoA for anterior and posterior keratometry readings, CCT, TCT, and ACD. Conclusion The findings provided by this study highlight that Scansys and Pentacam HR can be used interchangeably for anterior segment evaluation regarding most of the examined parameters.
Application of ERA5-Land reanalysis data in climate risk zoning for maize in the state of Bahia - Brazil
Authors: Matsunaga Wendy Kaori , Sales Eliton Sancler Gomes , Júnior Gervásio Costa Assis , Silva Madson Tavares , Lacerda Francinete Francis , Lima Evaldo Paiva , Santos Carlos Antonio Costa , Brito José Ivaldo Barbosa 
Published: January 2023

Abstract

Agricultural Zoning of Climate Risk (AZCR) is a fundamental tool for agricultural activities because it identifies regions and times of lower climate risk for planting and sowing crops, aiming to minimize the agricultural losses arising from climate hazards. In Brazil, AZCR has been used since 1996 as an official agricultural policy instrument. However, optimal AZCR representative of large areas require a network of meteorological stations with excellent spatial distribution, routine and accurate observations, and reliable data. Precipitation gauges that perform reliable measurements of precipitation have a good distribution in the Northeast region of Brazil. However, weather stations in this region are scarce. When weather stations are absent at a given location, input variables for AZCR are interpolated, which can lead to errors. Thus, seeking to solve the problem of the shortage of weather stations and improve the spatial distribution of information on meteorological variables, this study aimed to validate data from the ERA5-Land reanalysis of the European Center for Medium-Range Weather Forecasts and the CPC Precipitation Project. Once the data were validated, an AZCR was performed for the maize crop in the entire state of Bahia, in a 0.5° x 0.5° grid. It was observed that, with the exception of the far north mesoregion and a small area in the valley of Paraguaçu, the state of Bahia presented large areas suitable for planting of maize crops at different times of the year, with emphasis on areas in the southeast of the state that are suitable for planting all year round.
The sustained psychological impact of coronavirus disease 2019 pandemic on hospital workers 2 years after the outbreak: a repeated cross-sectional study in Kobe
Authors: Fukushima Haruko , Imai Hissei , Miyakoshi Chisato , Naito Atsumi , Otani Kyohei , Matsuishi Kunitaka 
Published: January 2023

Abstract
Background
Healthcare workers who are exposed to coronavirus disease 2019 are psychologically distressed. This study aimed to evaluate the mental health outcomes of hospital workers 2 years after the outbreak of coronavirus disease 2019 and to identify changes in the stress of hospital workers and predicted risk factors.
Methods
This survey was conducted 2 years after the initial evaluation performed under the first emergency declaration of the coronavirus disease 2019 pandemic among hospital workers at the same hospital in an ordinance-designated city in Japan from June to July 2022. Sociodemographic data, 19 stress-related question responses, the Impact of Event Scale-Revised, and the Maslach burnout inventory-general survey were collected. Multiple regression models were used to identify factors associated with each of the mental health outcomes 2 years after the coronavirus disease 2019 outbreak.
Results
We received 719 valid responses. Between 2020 and 2022, hospital workers’ anxiety about infection decreased, whereas their exhaustion and workload increased. Multiple regression analysis revealed that 2 years after the coronavirus disease 2019 outbreak, nurses and young people were at a higher risk of experiencing stress and burnout due to emotional exhaustion, respectively.
Conclusions
This is the first study to examine the long-term stress of hospital workers measured in Japan. Exhaustion and workload were worsened 2 years into the pandemic. Therefore, health and medical institutions should continuously monitor the physical and psychological health of staff members.
Modelling six sustainable development transformations and their accelerators, impediments, enablers, and interlinkages
Authors: Allen Cameron , Biddulph Annabel , Wiedmann Thomas , Pedercini Matteo , Malekpour Shirin 
Published: January 2023

Abstract

There is an urgent need to accelerate progress on the Sustainable Development Goals (SDGs) and recent research has identified six critical transformations. However, studies are yet to demonstrate how these transformations could be practically accelerated in a national context and what their combined effects would be. Here we deploy integrated systems modelling with transition storylines to elaborate and project six transformation pathways to the SDGs in Australia. By combining quantitative accelerators in the form of decisive policies and investments with storylines that diagnose common impediments and identify enabling conditions for systems change, our study advances knowledge on how the six transformations could be unlocked and accelerated. We find that conditions for transformation are emerging due to recent cascading crises, that feasible and affordable interventions and solutions are readily available that could trigger ‘S-shaped’ acceleration by 2030, and that continued long-term investment in climate action and resilience could stabilize progress towards sustainable wellbeing targets by 2050.