bluuebunny/crossref_metadata_2025_split huggingface dataset:
Hepatitis E virus seropositivity in an ethnically diverse community blood donor population
Authors: Mah Jordan K. , Keck MeiLe , Chu Daniel Y. , Sooryanarain Harini , Sahoo Malaya K. , Lau Patrick , Huang ChunHong , Weber Jenna , Belanger Geoffrey A. , Keck Zhenyong , Shan Hua , Meng Xiang‐Jin , Foung Steven K. H. , Pinsky Benjamin A. , Pham Tho D. 
Published: June 2023

AbstractBackground and ObjectivesHepatitis E virus (HEV) is an underrecognized and emerging infectious disease that may threaten the safety of donor blood supply in many parts of the world. We sought to elucidate whether our local community blood supply is at increased susceptibility for transmission of transfusion‐associated HEV infections.Materials and MethodsWe screened 10,002 randomly selected donations over an 8‐month period between 2017 and 2018 at the Stanford Blood Center for markers of HEV infection using commercial IgM/IgG serological tests and reverse transcriptase quantitative polymerase chain reaction assays (RT‐qPCR). Donor demographic information, including gender, age, self‐identified ethnicity, location of residence and recent travel, were obtained from the donor database and used to generate multivariate binary logistic regressions for risk factors of IgG seropositivity.ResultsA total of 10,002 blood donations from 7507 unique donors were screened, and there was no detectable HEV RNA by RT‐qPCR. The overall seropositivity rate was 12.1% for IgG and 0.56% for IgM. Multivariate analysis of unique donors revealed a significantly higher risk of IgG seropositivity with increasing age, White/Asian ethnicities and residence in certain local counties.ConclusionAlthough HEV IgG seroprevalence in the San Francisco Bay Area is consistent with ongoing infection, the screening of a large donor population did not identify any viraemic blood donors. While HEV is an underrecognized and emerging infection in other regions, there is no evidence to support routine blood screening for HEV in our local blood supply currently; however, periodic monitoring may still be required to assess the ongoing risk.
Cluster and Factor Analyses as Contributions to the Groundwater Quality Monitoring of the Marizal/São Sebastião Aquifer System, Alagoinhas (Bahia, Brazil)
Authors: Da Costa Maíra Sampaio , Gomes Maria da Conceição Rabelo , De Morais Nascimento Sérgio Augusto 
Published: June 2023

The Marizal/São Sebastião aquifer system is the main water supply of the municipality of Alagoinhas in the state of Bahia. However, anthropic interventions contribute to soil and groundwater pollution, increasing the need for related research. Multivariate statistical analysis is a widely used tool, helping in the investigation of groundwater quality while being capable of simultaneously evaluating diverse variables of a sample set. In this study, factor analysis and multivariate cluster analysis methodologies were applied. Ten of the most influential variables for groundwater quality were selected and then grouped into two factors. The first factor included electrical conductivity, salinity, calcium, chloride, sulfate, manganese, and iron, which are indicators of water salinity. The second factor encompassed pH, bicarbonate, and phosphate, indicating anthropic interventions and alkalinity in the environment. The multivariate cluster analysis was applied to the parameters of both factors, resulting in dendrograms with four clusters. The present study showed that the multivariate statistical analysis is an efficient tool for monitoring and can contribute to the management of groundwater quality.
Flight Path and Scheduling Effects on Perceived Jet Lag, Fatigue, and Sleep in Footballers Traveling to and From National Teams
Authors: Clements Ewan , Ehrmann Fabian , Clark Andrew , Jones Mark , McCall Alan , Duffield Rob 
Published: June 2023

Purpose: This study examined posttravel perceptual responses of national-team footballers (soccer) following different flight paths, arrival/departure times, and trip contexts. Methods: Details of 396 flights from national-team players (N = 68) were obtained and verified via an online flight database. Each player provided ratings of perceptual fatigue, sleep, soreness, stress, and jet lag for 2 days before and after each trip. The flight path (continents of departure and arrival), travel context (into vs out of national team), and arrival and departure times were obtained for each trip. Linear mixed models compared the pretravel with posttravel change in perceptual responses based on flight path, context, and schedule. Results: Perceived jet-lag ratings were more responsive to travel variables (R2 = .48) than other perceptual ratings (R2 < .26). Travel from Asia to Europe (P < .05) and Europe to Australia (P < .001) had significantly higher jet-lag ratings than all other paths. Fatigue scores were worst following Asia to Europe (P < .05) and Europe to Australia (P < .05) travel, while sleep scores were worst following Europe to Australia travel (P < .01). Perceptual responses were poorer following travel from national team to club compared with all other travel contexts (P < .05). Arrival during the daytime (11 AM to 5 PM) resulted in better perceptual responses than early-morning or late-night arrivals (P < .05). Conclusions: Perceived jet-lag ratings are more responsive to travel demands than perceptual wellness scales in national-team football athletes. Poorer perceptual responses may be expected when travel is longer in nature, ends later in the day, or involves travel out of the national team back to club.
SHIP1 modulates antimalarial immunity by bridging the crosstalk between type I IFN signaling and autophagy
Authors: Li Hongyu , Yang Shuai , Zeng Ke , Guo Jiayin , Wu Jian , Jiang Huaji , Xie Yingchao , Hu Zhiqiang , Lu Jiansen , Yang Jianwu , Su Xin-zhuan , Cui Jun , Yu Xiao 
Published: June 2023

ABSTRACT

Stringent control of the type I interferon (IFN-I) signaling is critical for host immune defense against infectious diseases, yet the molecular mechanisms that regulate this pathway remain elusive. Here, we show that Src homology 2 containing inositol phosphatase 1 (SHIP1) suppresses IFN-I signaling by promoting IRF3 degradation during malaria infection. Genetic ablation of
Ship1
in mice leads to high levels of IFN-I and confers resistance to
Plasmodium yoelii nigeriensis
(
P.y.
) N67 infection. Mechanistically, SHIP1 promotes the selective autophagic degradation of IRF3 by enhancing K63-linked ubiquitination of IRF3 at lysine 313, which serves as a recognition signal for NDP52-mediated selective autophagic degradation. In addition, SHIP1 is downregulated by IFN-I-induced miR-155-5p upon
P.y
. N67 infection and severs as a feedback loop of the signaling crosstalk. This study reveals a regulatory mechanism between IFN-I signaling and autophagy, and verifies SHIP1 can be a potential target for therapeutic intervention against malaria and other infectious diseases.

IMPORTANCE

Malaria remains a serious disease affecting millions of people worldwide. Malaria parasite infection triggers tightly controlled type I interferon (IFN-I) signaling that plays a critical role in host innate immunity; however, the molecular mechanisms underlying the immune responses are still elusive. Here, we discover a host gene [Src homology 2-containing inositol phosphatase 1 (SHIP1)] that can regulate IFN-I signaling by modulating NDP52-mediated selective autophagic degradation of IRF3 and significantly affect parasitemia and resistance of
Plasmodium
-infected mice. This study identifies SHIP1 as a potential target for immunotherapies in malaria and highlights the crosstalk between IFN-I signaling and autophagy in preventing related infectious diseases. SHIP1 functions as a negative regulator during malaria infection by targeting IRF3 for autophagic degradation.
Biophysical and proteomic analyses of
Pseudomonas syringae
pv.
tomato
DC3000 extracellular vesicles suggest adaptive functions during plant infection
Authors: Janda Martin , Rybak Katarzyna , Krassini Laura , Meng Chen , Feitosa-Junior Oséias , Stigliano Egidio , Szulc Beata , Sklenar Jan , Menke Frank L.H. , Malone Jacob G. , Brachmann Andreas , Klingl Andreas , Ludwig Christina , Robatzek Silke 
Published: June 2023

ABSTRACT

Vesiculation is a process employed by Gram-negative bacteria to release extracellular vesicles (EVs) into the environment. EVs from pathogenic bacteria play functions in host immune modulation, elimination of host defenses, and acquisition of nutrients from the host. Here, we observed EV production of the bacterial speck disease causal agent,
Pseudomonas syringae
pv.
tomato
(
Pto
) DC3000, as outer membrane vesicle release. Mass spectrometry identified 369 proteins enriched in
Pto
DC3000 EVs. The EV samples contained known immunomodulatory proteins and could induce plant immune responses mediated by bacterial flagellin. Having identified two biomarkers for EV detection, we provide evidence for
Pto
DC3000 releasing EVs during plant infection. Bioinformatic analysis of the EV-enriched proteins suggests a role for EVs in antibiotic defense and iron acquisition. Thus, our data provide insights into the strategies this pathogen may use to develop in a plant environment.

IMPORTANCE

The release of extracellular vesicles (EVs) into the environment is ubiquitous among bacteria. Vesiculation has been recognized as an important mechanism of bacterial pathogenesis and human disease but is poorly understood in phytopathogenic bacteria. Our research addresses the role of bacterial EVs in plant infection. In this work, we show that the causal agent of bacterial speck disease,
Pseudomonas syringae
pv.
tomato
, produces EVs during plant infection. Our data suggest that EVs may help the bacteria to adapt to environments, e.g., when iron could be limiting such as the plant apoplast, laying the foundation for studying the factors that phytopathogenic bacteria use to thrive in the plant environment.
Akkermansia muciniphila
and
Lactobacillus plantarum
ameliorate systemic lupus erythematosus by possibly regulating immune response and remodeling gut microbiota
Authors: Guo Mengchen , Lu Mei , Chen Kun , Xu Rui , Xia Yumin , Liu Xingyin , Liu Zhi , Liu Qisha 
Published: June 2023

ABSTRACT

Systemic lupus erythematosus (SLE), characterized by persistent inflammation, is a complex autoimmune disorder that affects all organs, challenging clinical treatment. Dysbiosis of gut microbiota promotes autoimmune disorders that damage extraintestinal organs. Modulating the gut microbiome is proposed as a promising approach for fine-running parts of the immune system, relieving systematic inflammation in multiple diseases. This study demonstrated that the administration of
Akkermansia muciniphila
and
Lactobacillus plantarum
contributed to an anti-inflammatory environment by decreasing IL-6 and IL-17 and increasing IL-10 levels in the circulation. The treatment of
A. muciniphila
and
L. plantarum
restored the intestinal barrier integrity to a different extent. In addition, both strains reduced the deposit of IgG in the kidney and improved renal function significantly. Further studies revealed distinct remodeling roles of
A. muciniphila
and
L. plantarum
administration on the gut microbiome. This work demonstrated essential mechanisms of how
A. muciniphila
and
L. plantarum
remodel gut microbiota and regulate the immune responses in the SLE mice model.

IMPORTANCE

Several pieces of research have demonstrated that certain probiotic strains contribute to regulating excessive inflammation and restoring tolerances in the SLE animal model. More animal trials combined with clinical studies are urgently needed to further elucidate the mechanisms for the effect of specific probiotic bacteria in preventing SLE symptoms and developing novel therapeutic targets. In this study, we explored the role of
A. muciniphila
and
L
.
plantarum
in ameliorating the SLE disease activity. Both
A. muciniphila
and
L. plantarum
treatment relieved the systemic inflammation and improved renal function in the SLE mouse model. We demonstrated that
A. muciniphila
and
L. plantarum
contributed to an anti-inflammatory environment by regulating cytokine levels in the circulation, restoring the intestinal barrier integrity, and remodeling the gut microbiome, however, to a different extent.
Can mediation avoid litigation in conflicts about medical treatment for children? An analysis of previous litigation in England and Wales
Authors: Neefjes Veronica 
Published: June 2023

ObjectiveTo investigate the reasons why parents disagree with their clinicians in cases reaching the court and to estimate the number of cases in which mediation might have avoided litigation.DesignAnalysis of 83 published cases regarding medical treatment decisions for children initiated either by an NHS Trust or Local Authority between 1990 and 1 July 2022.ResultsThe analysis found that the main areas of contention are different value judgements, different interpretations of observable events such as the health of the child, their quality of life or burden of treatment and relational issues (ie, loss of trust). More than half of the cases are estimated not to have been preventable by mediation because either no conflict existed (n=13) or the parental decision was based on strongly held, mostly faith-based, views unlikely to be open for discussion (n=31).ConclusionThe potential of mediation to avoid future litigation may be more limited than hoped for.
Mask ventilation using volume-targeted neonatal ventilator for neonatal resuscitation: a randomised cross-over simulation study
Authors: Law Brenda Hiu Yan , Madani Kia Tina , Trinh Faith , Schmölzer Georg M 
Published: June 2023

ObjectiveTo use simulations to compare a novel mask ventilation method using a neonatal ventilator, with mask ventilation using a T-piece resuscitator, to study human factors prior to clinical testing.DesignProspective randomised cross-over simulation study. Participants were briefly trained to use a neonatal ventilator for mask ventilation. Each participant was fitted with eye-tracking glasses to record visual attention (VA) and performed two simulated preterm neonatal resuscitations in a randomised sequence.SettingIn situ in a neonatal resuscitation room within a Level 3 neonatal intensive care unit.ParticipantsHealthcare professionals (HCPs) trained in neonatal resuscitation with experience as team leaders.InterventionsSemiautomated, ventilator-based, volume-targeted positive pressure mask ventilation (VTV-PPV) versus manual mask ventilation via T-piece device (T-piece PPV).Main outcome measuresSubjective workload (Surgical Task Load Index, SURG-TLX), VA, quantitative and qualitative postsimulation survey responses.ResultsThirty HCPs participated. HCPs reported higher total SURG-TLX scores (43.5/120 vs 33.8/120) and higher scores in mental demand (8.2/20 vs 5.6/20), physical demand (6.6/20 vs 5.1/20), task complexity (8.2/20 vs 6/20) and situational stress (8.3/20 vs 5.9/20) for VTV-PPV. Temporal demand and distraction scores were similar. While participants took longer to complete VTV-PPV simulations, participants dedicated similar a %VA to the mannikin and T-piece gauges or ventilator screen. More participants increased the rate of ventilation during VTV-PPV; other corrective steps were similar. Overall, participants rated VTV-PPV positively. Participants identified potential challenges with physical ergonomics, cognition and teamwork.ConclusionUsing a neonatal ventilator to perform volume-targeted PPV is feasible, but human factors need to be considered.
Epidemiology of Robin sequence in the UK and Ireland: an active surveillance study
Authors: Wright Marie FA , Knowles Rachel L , Cortina-Borja Mario , Javadpour Sheila , Mehendale Felicity V , Urquhart Donald S 
Published: June 2023

BackgroundBirth prevalence of Robin sequence (RS) is commonly reported as 1 case per 8000–14 000 live births. These estimates are based on single-source case ascertainment and may miss infants who did not require hospital admission or those without overt upper airway obstruction at birth.ObjectivesTo identify the true birth prevalence of RS with cleft palate in the UK and Ireland from a population-based birth cohort with high case ascertainment.MethodsActive surveillance of RS with cleft palate was carried out in the UK/Ireland using dual sources of case ascertainment: British Paediatric Surveillance Unit (BPSU) reporting card and nationally commissioned cleft services. Clinical data were collected from notifying clinicians at two time points.Results173 live-born infants met the surveillance case definition, giving a birth prevalence of 1 case per 5250 live births (19.1 per 100 000 (95% CI 16.2 to 21.9)), and 1:2690 in Scotland. 47% had non-isolated RS, with Stickler syndrome the most common genetic diagnosis (12% RS cases). Birth prevalence derived from the combined data sources was significantly higher than from BPSU surveillance alone.ConclusionsBirth prevalence of RS in the UK/Ireland derived from active surveillance is higher than reported by epidemiological studies from several other countries, and from UK-based anomaly registries, but consistent with published retrospective data from Scotland. Dual case ascertainment sources enabled identification of cases with mild or late-onset airway obstruction that were managed without hospital admission. Studies of aetiology and equivalent well-designed epidemiological studies from other populations are needed to investigate the identified geographical variability in birth prevalence.
Yoga-based interventions may reduce anxiety symptoms in anxiety disorders and depression symptoms in depressive disorders: a systematic review with meta-analysis and meta-regression
Authors: Martínez-Calderon Javier , Casuso-Holgado María Jesús , Muñoz-Fernandez Maria Jesus , Garcia-Muñoz Cristina , Heredia-Rizo Alberto Marcos 
Published: June 2023

ObjectiveTo summarise the effect of mind–body exercises on anxiety and depression symptoms in adults with anxiety or depressive disorders.DesignSystematic review with meta-analysis and meta-regression.Data sourcesFive electronic databases were searched from inception to July 2022. Manual searches were conducted to explore clinical trial protocols, secondary analyses of clinical trials and related systematic reviews.Eligibility criteriaRandomised clinical trials evaluating qigong, tai chi or yoga styles with anxiety or depression symptoms as the outcomes were included. No intervention, waitlist or active controls were considered as control groups. The risk of bias and the certainty of the evidence were assessed. Meta-analyses, meta-regressions and sensitivity analyses were performed.Results23 studies, comprising 22 different samples (n=1420), were included. Overall, meta-analyses showed yoga interventions were superior to controls in reducing anxiety symptoms in anxiety disorders. Furthermore, yoga-based interventions decreased depression symptoms in depressive disorders after conducting sensitivity analyses. No differences between groups were found in the rest of the comparisons. However, the certainty of the evidence was judged as very low for all outcomes due to concerns of high risk of bias, indirectness of the evidence, inconsistency and imprecision of the results. In addition, there was marked heterogeneity among yoga-based interventions and self-reported tools used to evaluate the outcomes of interest.ConclusionAlthough yoga-based interventions may help to improve mental health in adults diagnosed with anxiety or depressive disorders, methodological improvements are needed to advance the quality of clinical trials in this field.PROSPERO registration numberCRD42022347673.
Prehospital transdermal glyceryl trinitrate in patients with ultra-acute presumed stroke (RIGHT-2): effects on outcomes at day 365 in a randomised, sham-controlled, blinded, phase III, superiority ambulance-based trial
Authors: Woodhouse Lisa J , Appleton Jason P , Ankolekar Sandeep , England Timothy J , Mair Grant , Muir Keith , Price Christopher I , Pocock Stuart , Randall Marc , Robinson Thompson G , Roffe Christine , Sandset Else C , Saver Jeffrey L , Siriwardena Aloysius Niroshan , Sprigg Nikola , Wardlaw Joanna M , Bath Philip M 
Published: June 2023

BackgroundThe Rapid Intervention with Glyceryl Trinitrate in Hypertensive Stroke Trial-2 (RIGHT-2) reported no overall treatment difference between glyceryl trinitrate (GTN) and sham at day 90. Here we assess participants’ outcomes 1 year after randomisation.MethodsRIGHT-2 was an ambulance-based prospective randomised controlled trial where patients with presumed stroke and systolic blood pressure (BP) of >120 mm Hg received either GTN (5 mg/day) or sham patch. Centralised blinded telephone follow-up was performed at days 90 (primary endpoint) and 365 (secondary endpoint). The lead outcome was dependency assessed with the modified Rankin Scale (mRS).Results1149 patients were recruited to RIGHT-2 between October 2015 and May 2018, and 1097 (95.5%) had outcome data recorded at day 365. At baseline, the patients were; female (48%), had a mean age of 73 (15) years, BP of 162 (25)/92 (18) mm Hg, onset to randomisation of 70 (45–115) min, diagnosis of ischaemic stroke (52%), intracerebral haemorrhage (ICH) (13%), transient ischaemic attack (TIA) (9%) and mimics (26%). There was no effect of GTN on mRS score at day 365 in participants with confirmed stroke/TIA (adjusted common odds ratio (acOR) 1.10, 95% CI 0.86 to 1.42) or in all patients. In patients randomised to GTN, mRS at day 365 tended to be worse in those with ICH (acOR 1.65, 95% CI 0.84 to 3.25) and better in those with a mimic diagnosis (acOR 0.53, 95% CI 0.33 to 0.84).ConclusionAt 1 year post randomisation, dependency did not differ between GTN and sham treatment in either the target population or overall. In prespecified subgroup analyses, GTN was associated with reduced dependency in participants with a final diagnosis of mimic and a non-significant worse outcome in participants with ICH.Trial registration numberISRCTN26986053.
Association between chronic obstructive pulmonary disease and periodontal disease: a systematic review and meta-analysis
Authors: Yang Mei , Peng Ran , Li Xiaoou , Peng Junjie , Liu Lin , Chen Lei 
Published: June 2023

ObjectivesStudies have suggested contradictory results on the relationship between chronic obstructive pulmonary disease (COPD) and periodontal disease (PD). The aim of this study was to determine whether PD increased the risk of COPD and COPD-related clinical events.DesignA systematic review and meta-analysis.Data sourcesPubMed, Ovid EMBASE and Ovid CENTRAL were searched from inception to 22 February 2023.Eligibility criteria for studiesWe included trials and observational studies evaluating association of PD with the risk of COPD or COPD-related events (exacerbation and mortality), with statistical adjustment for smoking.Data extraction and synthesisTwo investigators independently extracted data from selected studies using a standardised Excel file. Quality of studies was evaluated using the Newcastle-Ottawa Scale. OR with 95% CI was pooled in a random-effect model with inverse variance method.Results22 observational studies with 51 704 participants were included. Pooled analysis of 18 studies suggested that PD was weakly associated with the risk of COPD (OR: 1.20, 95% CI 1.09 to 1.32). However, in stratified and subgroup analyses, with strict adjustment for smoking, PD no longer related to the risk of COPD (adjusting for smoking intensity: OR: 1.14, 95% CI 0.86 to 1.51; smokers only: OR: 1.46, 95% CI 0.92 to 2.31; never smokers only: OR: 0.93, 95% CI 0.72 to 1.21). Moreover, PD did not increase the risk of COPD-related exacerbation or mortality (OR: 1.18, 95% CI 0.71 to 1.97) in the pooled result of four studies.ConclusionsThis study demonstrates PD confers no risk for COPD and COPD-related events when strictly adjusted by smoking. Large-scale prospective cohort studies with control of potential confounding factors are warranted to validate the present findings.