bluuebunny/crossref_metadata_2025_split huggingface dataset:
Taxonomic study of Scytosiphon (Phaeophyceae) from temperate coasts of Argentina
Authors: Croce Maria Emilia , Hoshino Masakazu , Gauna Maria Cecilia , Parodi Elisa R. , Kogame Kazuhiro 
Published: January 2023

AbstractScytosiphon is a common intertidal genus widely distributed on temperate coasts worldwide. Recently, eight species have been delimited with molecular tools. Although S. lomentaria is the only species that predominates in the macroalgal literature of the Southwestern Atlantic Ocean (SwAO), unpublished molecular data obtained for a population study of S. lomentaria revealed hidden species diversity of Scytosiphon among the individuals collected from four localities at the SwAO. The aim of this study was to revise the identity and phylogenetic relationships of Scytosiphon from temperate coasts of the SwAO using DNA data. Thalli were collected from the Argentinean coast between 39° S and 43° S, from which cox1 and rbcL gene sequences were obtained. Phylogenies and haplotype networks were inferred and morphology of gametophytes was studied. Four species were recognized, S. lomentaria, S. promiscuus, S. shibazakiorum, and one species that belongs to a complex of species known as “Scytosiphon Atlantic complex.” This complex was known to occur only in the North Atlantic, however, the results found in this study revealed that it has an extended distribution range that includes the southern hemisphere, where its populations have high genetic diversity and unique haplotypes. The morphological differences among the four species were subtle; denoting that previous Scytosiphon records from the SwAO attributed to the renowned S. lomentaria could represent different species. In addition, sex ratio and genome‐wide single nucleotide polymorphisms (SNPs) analyses were done for populations of S. promiscuus presumably introduced to the SwAO, and the results indicated that they included female‐dominant parthenogenetic populations, which were probably introduced from Japan.
Phrase Rhythm and Loss in the Music of Maurice Ravel
Authors: Blättler Damian 
Published: January 2023

ABSTRACTA particular subset of Ravel's output features a phrase‐rhythmic technique wherein tonal and thematic returns are accompanied by surprisingly asymmetrical or ambiguous phrase rhythm. This defies both generic conventions linking thematic reprise and tonal closure to relatively stable phrase rhythm and specific expectations created by these works’ formal processes, and contrasts with trajectories moving from phrase‐rhythmic instability to stability which Ravel deploys in other works. The set of pieces which features this technique includesÀ la manière de … Chabrier, theMenuet sur le nom d'Haydn, pieces fromLe Tombeau de Couperin, the last of theValses nobles et sentimentales, and the ‘Blues’ movement from the Violin Sonata. This study notes how themes of loss and distance connect these pieces, allowing for the phrase‐rhythmic technique to be bound up with interpretative implications which can enhance our understanding of how phrase rhythm can carry expressive freight.
Pathogenicity of phospholipase B1 of Trichosporon asahii in immunosuppressed mice
Authors: Cheng Xiaoxian , Zhu He , Bai Shuang , Zou Yuekun , Xia Zhikuan , Yang Rongya 
Published: January 2023

AbstractBackgroundTrichosporon asahii is an opportunistic pathogenic yeast‐like fungus. Phospholipase B1 (PLB1) is an important virulence factor of pathogenic fungi such as Candida albicans and Cryptococcus neoformans, and there are few studies on the role of PLB1 in the pathogenicity of T. asahii.ObjectivesTo investigate the role of PLB1 in the pathogenicity of T. asahii.MethodsA strain with low secretion of PLB1 (4848) was screened, a PLB1 overexpression strain (PLB1OX) was constructed, and the differences in histopathology, fungal load of organ, survival time of mice, the levels of IL‐6, IL‐10, TNF‐α, and GM‐GSF in the serum and organs caused by the two strains were compared.ResultsHistopathology showed that spores and hyphae were observed in both groups, and PLB1OX led to more fungal invasion. The fungal loads in the kidney, lung, spleen and liver in the PLB1OXgroup were significantly higher than those in the 4848 group, and the survival time of mice was significantly lower than that in the 4848 group. The levels of TNF‐α in the serum, liver, spleen, lung and kidney of the PLB1OX group were lower than those of the 4848 group, while the level of IL‐10 in the serum was higher than that of the 4848 group.ConclusionsThese results suggest that PLB1 can enhance the invasive function of T. asahii and affect the secretion of TNF‐α and IL‐10 which may affect the host antifungal immune response, providing evidence that PLB1 plays a role in the pathogenic infection of T. asahii.
Continuous dosing of nikkomycin Z against systemic candidiasis, in vivo and in vitro correlates
Authors: Sass Gabriele , Larwood David J. , Martinez Marife , Stevens David A. 
Published: January 2023

AbstractBackgroundCandidiasis is the most common cause of fungal sepsis, and new agents are of interest to ameliorate current deficiencies in therapy. Nikkomycin Z (NIKZ) is an inhibitor of chitin synthase, interfering with fungal cell wall development.Objectives/MethodsWe studied NIKZ therapy of disseminated murine candidiasis, via continuous drug exposure, in drinking water, to compensate for rapid clearance of the drug.ResultsDrinking, and thus drug intake in the NIKZ groups, as well as body weight, was affected by the degree of illness. NIKZ effect on survival, despite reduced drinking initially after infection, was highly efficacious and dose‐related, and comparable to fluconazole, though neither were curative with the regimens employed. The challenge was rapidly lethal to all untreated animals, whereas NIKZ groups achieved >50% survival. Assays of residual fungal infection were consistent with impressions of efficacy based on survival. Although NIKZ MIC for Candida albicans appeared unpromising, mycelial formation assays more closely correlated with in vivo observations.ConclusionsIn vitro‐in vivo disparity may be explained by NIKZ tissue concentration in the target tissue and/or by enhanced NIKZ action on mycelial formation, a morphological change in vivo wherein chitin synthesis is more critical, compared to NIKZ activity in inhibiting planktonic growth. A sustained release oral form of NIKZ in drug development for humans could hold promise, possibly also in future exploring previously demonstrated synergy in vitro with other antifungals.
Clinical diagnostic criteria of benign adult familial myoclonus epilepsy type 1 are highly concordant with genetic testing
Authors: Ishibashi Haruka , Kobayashi Katsuya , Tojima Maya , Neshige Shuichiro , Hitomi Takefumi , Ishiura Hiroyuki , Tsuji Shoji , Maruyama Hirofumi , Takahashi Ryosuke , Ikeda Akio 
Published: January 2023

AbstractBackgroundThe clinical diagnostic criteria for benign adult familial myoclonus epilepsy (BAFME) originally included (1) cortical tremor and infrequent generalized seizures, (2) autosomal dominant inheritance, (3) lack of cognitive decline and other neurological symptoms, (4) electrophysiological findings of cortical reflex myoclonus, and (5) lack of clear clinical progression (BAFME criteria‐1). It was revised such that (1) included partial seizures, and (3) and (5) may develop among middle‐aged patients (Revised criteria‐2). The Japanese Ministry of Health, Labor and Welfare proposed their criteria, which included the EEG and MRI findings (MHLW criteria‐3). Recently, high‐frequency oscillations, superimposed on the giant somatosensory evoked potential P25 component (P25‐HFOs), have been found useful as a biomarker for BAFME diagnosis.AimWe examined the genetic diagnosis of BAFME type 1 and its consistency with the three diagnostic criteria and P25‐HFOs.MethodsTwenty‐four Japanese patients, who underwent BAFME genetic testing, were rated using three independent diagnostic criteria and P25‐HFOs.ResultsTwenty‐one patients were genetically diagnosed with BAFME type 1. Nineteen patients fulfilled BAFME‐1 (sensitivity 90%), and 21 fulfilled Revised‐2 and MHLW criteria‐3 (sensitivity 100%). We could evaluate P25‐HFOs in 19 of the 21 gene‐positive patients, and 17 of the 19 patients showed P25‐HFOs. Three patients with negative genetic testing did not meet any of the criteria and had no P25‐HFOs.ConclusionsThe three available clinical diagnostic criteria for BAFME were highly concordant with the positive result for genetic testing.
Rickettsial infection‐related encephalitis/encephalopathy with reversible splenial lesion of the corpus callosum
Authors: Houssem Slimène , Khadija Sonda Moalla , Salma Sakka , Nouha Farhat , Mariem Damak , Chokri Mhiri 
Published: January 2023

AbstractMild encephalopathy with reversible splenial lesion (MERS) is a rare clinicoradiological entity characterized by a transient mild encephalopathy and MRI findings of a reversible lesion in the splenium of the corpus callosum. Here, we report an atypical case of MERS in a 22‐year‐old woman who presented with 2‐day history of headaches and confusion associated with fever and who completely recovered within 7 days. Exhaustive biological tests panel revealed positive IgM rickettsial antibody, and the final diagnosis was MERS caused by rickettsial infection. Although viruses are the most common pathogen of MERS, for proper management, bacteria should be considered, as they may also lead to this condition.
Artificial intelligence‐assisted determination of available sites for palatal orthodontic mini implants based on palatal thickness through CBCT
Authors: Tao Tianjin , Zou Ke , Jiang Ruiyi , He Ketai , He Xian , Zhang Mengyun , Wu Zhouqiang , Shen Xiaojing , Yuan Xuedong , Lai Wenli , Long Hu 
Published: January 2023

AbstractObjectivesTo develop an artificial intelligence (AI) system for automatic palate segmentation through CBCT, and to determine the personalized available sites for palatal mini implants by measuring palatal bone and soft tissue thickness according to the AI‐predicted results.Materials and MethodsEight thousand four hundred target slices (from 70 CBCT scans) from orthodontic patients were collected, labelled by well‐trained orthodontists and randomly divided into two groups: a training set and a test set. After the deep learning process, we evaluated the performance of our deep learning model with the mean Dice similarity coefficient (DSC), average symmetric surface distance (ASSD), sensitivity (SEN), positive predictive value (PPV) and mean thickness percentage error (MTPE). The pixel traversal method was proposed to measure the thickness of palatal bone and soft tissue, and to predict available sites for palatal orthodontic mini implants. Then, an example of available sites for palatal mini implants from the test set was mapped.ResultsThe average DSC, ASSD, SEN, PPV and MTPE for the segmented palatal bone tissue were 0.831%, 1.122%, 0.876%, 0.815% and 6.70%, while that for the palatal soft tissue were 0.741%, 1.091%, 0.861%, 0.695% and 12.2%, respectively. Besides, an example of available sites for palatal mini implants was mapped according to predefined criteria.ConclusionsOur AI system showed high accuracy for palatal segmentation and thickness measurement, which is helpful for the determination of available sites and the design of a surgical guide for palatal orthodontic mini implants.
Treatment with an inhibitor of matrix metalloproteinase 9 or cathepsin K lengthens embryonic lower jaw bone
Authors: Houchen Claire J. , Castro Bethany , Hahn Leat Portia , Mohammad Nashwa , Hall‐Glenn Faith , Bumann Erin E. 
Published: January 2023

AbstractObjectivesSkeletal malocclusions are common, and severe malocclusions are treated by invasive surgeries. Recently, jaw bone length has been shown to be developmentally controlled by osteoclasts. Our objective was to determine the effect of inhibiting osteoclast‐secreted proteolytic enzymes on lower jaw bone length of avian embryos by pharmacologically inhibiting matrix metalloproteinase‐9 (MMP9) or cathepsin K (CTSK).MethodsQuail (Coturnix coturnix japonica) embryos were given a single dose of an inhibitor of MMP9 (iMMP9), an inhibitor CTSK (iCTSK), or vehicle at a developmental stage when bone deposition is beginning to occur. At a developmental stage when the viscerocranium is largely calcified, the heads were scanned via micro‐computed tomography and reproducible landmarks were placed on 3D‐reconstructed skulls; the landmark coordinates were used to quantify facial bone dimensions.ResultsApproximately half of the quail given either iMMP9 or iCTSK demonstrated an overt lower jaw phenotype, characterized by longer lower jaw bones and a greater lower to upper jaw ratio than control embryos. Additionally, iMMP9‐treated embryos exhibited a significant change in midface length and iCTSK‐treated embryos had significant change in nasal bone length.ConclusionMMP9 and CTSK play a role in osteoclast‐mediated determination of lower jaw bone length. Pharmacological inhibition of MMP9 or CTSK may be a promising therapeutic alternative to surgery for treating skeletal jaw malocclusions, but more preclinical research is needed prior to clinical translation.
Genetic associations between gene polymorphisms on 3p25 and oral squamous cell carcinoma
Authors: Yuan Minyan , Zheng Xueqing , Jing Jiaojiao , Li Yang , Liu Nianke , Song Yaling 
Published: January 2023

AbstractObjectivesTo evaluate the association of SYN2, PPARG, RAF1, TIMP4, and IQSEC1 polymorphisms in 3p25 with oral squamous cell carcinoma (OSCC) in the Chinese Han population.Subjects and MethodsGenomic DNA was extracted from 494 subjects with or without OSCC. Basic information on the subjects, clinical data, and prognoses were collected. Fifteen candidate single nucleotide polymorphisms (SNPs) were selected and genotyped. The statistical analyses included descriptive statistics, logistic regression, survival, and functional annotation was performed.ResultsIQSEC1‐rs2686742 correlated with OSCC occurrence. In addition, RAF1‐rs1051208, PPARG‐rs10865710, PPARG‐rs3856806, IQSEC1‐rs2686742, PPARG‐rs1175544, IQSEC1‐rs9211, and IQSEC1‐rs2600322 were significantly associated with the clinical characteristics of patients with OSCC. The log‐rank test showed that IQSEC1‐rs2600322 may play an important role in the survival of patients with OSCC. The Cox regression analysis suggested that PPARG‐rs10865710, PPARG‐rs7649970, IQSEC1‐rs9211, IQSEC1‐rs2600322, and IQSEC1‐rs12487715 influenced survival outcomes. The functional annotation indicated that the transcript levels of IQSEC1 were upregulated in head and neck squamous cell carcinoma tissues, whereas PPARG gene transcription was downregulated.ConclusionsIQSEC1‐rs2686742 may be closely associated with OSCC onset. Multiple SNPs in IQSEC1 and PPARG genes correlated with the clinical characteristics of OSCC, among which PPARG‐rs10865710, IQSEC1‐rs9211, and IQSEC1‐rs2600322 were associated with cancer prognosis.
Relationship between oral lesions and severe SARS‐CoV‐2 infection in intensive care unit patients
Authors: Favia Gianfranco , Barile Giuseppe , Tempesta Angela , Copelli Chiara , Novielli Gianluigi , Dell'Olio Fabio , Capodiferro Saverio , Spirito Francesca , Brienza Nicola , Ribezzi Mario , Vestito Maria Concetta , Corriero Alberto , Carpagnano Elisiana , Moschetta Antonio , Chironna Maria , Loconsole Daniela , Centrone Francesca , Quadri Mir Faeq Ali , Tartaglia Gianluca Martino , Limongelli Luisa 
Published: January 2023

AbstractObjectiveOral lesions received increased attention as likely new signs or secondary manifestations of COVID‐19. Therefore, we clinically examined oral cavity of patients with COVID‐19 and investigated oral lesions and patient comorbidities as possible risk factors of COVID‐19 disease outcome.MethodsFrom January to March 2022, a prospective study was conducted by recruiting all COVID‐19 patients admitted to the Intensive Care Unit and Respiratory Intensive Care Unit of Maxi‐Emergencies Hospital in Bari, Italy.ResultsFrom the enrolled 103 COVID‐19 patients, 46.6% were females and 53.4% were males. Findings show that risk of presenting with severe COVID‐19 disease was higher in patients who developed oral lesions related to COVID‐19 than those with no oral lesions (RR = 7.998, p = .002). Next, patients with concomitant autoimmune diseases were at higher risk of a negative COVID‐19 disease outcome than those without comorbidities (OR = 8.838, p = .026).ConclusionsCOVID‐19‐related lesions of oral mucosa should not be ignored as they can be early and easily detectable signs of severe COVID‐19 disease condition, thus, serving as a prevention measure for any potential unfortunate event. Findings of this study, without implying causation, offer a direction for future investigations that aim to confirm the presence of specific oral lesions in COVID‐19 patients as signs of severe disease progression.
Psychological distress with relationship satisfaction is moderated by anticipatory relational savoring among non‐deployed military partners
Authors: Froidevaux Nicole M. , Sanchez Hernandez Hugo , Pourmand Vida , Yunusova Asal , Sbarra David A. , Borelli Jessica L. 
Published: January 2023

AbstractThis study recruited participants whose partners were deployed for active‐duty military service to examine whether anticipatory relational savoring moderated the association of psychological distress with relationship satisfaction. Two weeks prior to their partner's deployment (T1), participants (N = 73) completed a self‐report measure of relationship satisfaction. Then, 2 weeks into their partner's deployment (T2), participants completed self‐report measures of stress, loneliness, and depression (combined into a composite index of psychological distress), and relationship satisfaction. Participants also completed a stream‐of‐consciousness task at T2 in which they imagined and discussed their partner's return from deployment. We coded the stream‐of‐consciousness task for anticipatory relational savoring regarding their upcoming reunion with their deployed partner. We found that anticipatory relational savoring moderated the association of psychological distress with during‐deployment relationship satisfaction after adjusting for demographics, interpersonal variables, and deployment‐specific variables; the association did not hold after adjusting for pre‐deployment relationship satisfaction, and thus was robust when considering the distress‐satisfaction association during the deployment but was not when considering changes in relationship satisfaction from pre‐ to during‐deployment. We discuss the potential importance of anticipatory relational savoring for this unique population.
Expanding growers' choice of plant disease management options can promote suboptimal social outcomes
Authors: Murray‐Watson Rachel E. , Cunniffe Nik J. 
Published: January 2023

AbstractPrevious models of growers' decision‐making during epidemics have unrealistically limited disease management choices to just two options. Here, we expand previous game‐theoretic models of grower decision‐making to include three control options: a crop that is tolerant, resistant or susceptible to disease. Using tomato yellow leaf curl virus (TYLCV) as a case study, we investigate how growers can be incentivized to use different control options to achieve socially optimal outcomes. To do this, we consider the efforts of a ‘social planner’ who moderates the price of crops. We find that subsidizing a tolerant crop costs the social planner more in subsidies, as its use encourages selfishness and widespread adoption. Subsidizing a resistant crop, however, provides widespread benefits by reducing the prevalence of disease across the community of growers, including those that do not control, reducing the number of subsidies required from the social planner. We then use Gini coefficients to measure equitability of each subsidization scheme. This study highlights how grower behaviour can be altered using crop subsidies to promote socially optimal outcomes during epidemics.
Manifestations of B‐cell expansion with NF‐κB and T‐cell anergy disease overlapping with hemophagocytic lymphohistiocytosis
Authors: Xiao Haijuan , Mou Wenjun , Zhang Rui , Guo Xin , Chen Heying , Zhang Liqiang , Liu Gang , Gui Jingang 
Published: January 2023

AbstractB cell expansion with NF‐κB and T cell anergy (BENTA) is a disease genetically linked with heterozygous gain‐of‐function (GOF) mutations in the CARD11 gene with an autosomal dominant expression. Hemophagocytic lymphohistiocytosis (HLH) is a heterogeneous group of disorders characterized by systemic inflammation and hypercytokinaemia. Some BENTA patients share similar clinical manifestations as HLH in various aspects, including fever and splenomegaly. In this study, we reported a 15‐month‐old boy diagnosed as BENTA meeting with diagnostic criteria of HLH. The complications were resolved by antibiotics for controlling severe infection, together with the reduced format of dexamethasone and etoposide for subsiding HLH activities. While the patient was not subjected to disease recurrence and maintained free of infection, a persistent lymphocytosis derived mainly from the expansion of polyclonal B cells was ascertained. Flow cytometry analysis indicated that the subdued degranulation of NK cells prior to treatment had been restored as the HLH‐related complications waned. With largely reduced number and ratios in CD4 and CD8 T cells, their proliferation and Vβ diversity remained in normal ranges. In vitro stimulation experiment revealed a functional abbreviation of T cells as the percentage of IFNγ‐releasing CD3+CD4+ T cells augmented while the percentage reduced in CD3+CD4− T cells. Whole exome sequencing revealed a de novo G123D missense mutation in the CARD11 gene. This new case of BENTA showcased a scenario of predominant HLH activities accompanied by a severe infection normally associated with BENTA. In addition, a brief treatment quenching HLH complication in cooperation with antibiotics for infection control was not able to solve the underlined T cell abnormality as well as B cell expansion caused by CARD11 mutation. A haematopoietic stem cell transplantation or gene therapy is still a pursuit to remedy this inborn error of immunity.
The accuracy of markerless motion capture combined with computer vision techniques for measuring running kinematics
Authors: Van Hooren Bas , Pecasse Noah , Meijer Kenneth , Essers Johannes Maria Nicolaas 
Published: January 2023

AbstractBackgroundMarkerless motion capture based on low‐cost 2‐D video analysis in combination with computer vision techniques has the potential to provide accurate analysis of running technique in both a research and clinical setting. However, the accuracy of markerless motion capture for assessing running kinematics compared to a gold‐standard approach remains largely unexplored.ObjectiveHere, we investigate the accuracy of custom‐trained (DeepLabCut) and existing (OpenPose) computer vision techniques for assessing sagittal‐plane hip, knee, and ankle running kinematics at speeds of 2.78 and 3.33 m s−1 as compared to gold‐standard marker‐based motion capture.MethodsDifferences between the markerless and marker‐based approaches were assessed using statistical parameter mapping and expressed as root mean squared errors (RMSEs).ResultsAfter temporal alignment and offset removal, both DeepLabCut and OpenPose showed no significant differences with the marker‐based approach at 2.78 m s−1, but some significant differences remained at 3.33 m s−1. At 2.78 m s−1, RMSEs were 5.07, 7.91, and 5.60, and 5.92, 7.81, and 5.66 degrees for the hip, knee, and ankle for DeepLabCut and OpenPose, respectively. At 3.33 m s−1, RMSEs were 7.40, 10.9, 8.01, and 4.95, 7.45, and 5.76 for the hip, knee, and ankle for DeepLabCut and OpenPose, respectively.ConclusionThe differences between OpenPose and the marker‐based method were in line with or smaller than reported between other kinematic analysis methods and marker‐based methods, while these differences were larger for DeepLabCut. Since the accuracy differed between individuals, OpenPose may be most useful to facilitate large‐scale in‐field data collection and investigation of group effects rather than individual‐level analyses.