bluuebunny/crossref_metadata_2025_split huggingface dataset:
Predicting depressive symptoms and psychological distress by circulating inflammatory mediators: A 16-month prospective study in Japanese white-collar employees
Authors: Soeishi Takahiro , Nakata Akinori , Nagata Tomohisa , Akutsu Satoshi , Tondokoro Tsukumi , Inoue Yukiko , Katsumura Fumiaki , Kikunaga Kazuki 
Published: January 2025

ABSTRACT

Objective
Although increasing evidence suggests that depression/distress involves inflammatory processes, its potential sex differences and the temporal directions for this association remain elusive.

Methods
We examined the temporal association between serum inflammatory mediators and depression/distress as measured by the Center for Epidemiologic Studies Depression Scale (CES-D) and the Kessler Psychological Distress Scale (K6), in non-depressed working men and women (n = 61 and 43, respectively) by a 16-month prospective design.

Results
Fully-adjusted partial correlation analyses revealed that in men, a lower IFN-γ predicted subsequent increases in CES-D and K6 scores, while a higher TNF-α predicted increased K6 scores. In women, a higher IFN-γ predicted a subsequent increase in the CES-D score. CES-D and K6 scores did not predict inflammatory mediators at follow-up.

Conclusions
The finding suggests that inflammatory activation precedes depression/distress with distinct sex differences.
The Relationship Between Longest-Held Occupation and Hand Function in Older Adults
Authors: Levinson Jaden E. , Logue Cook Rachel N. , Brown Susan H. 
Published: January 2025

ABSTRACT

Objective: Hand function, an important component of daily functioning, declines with age, yet the degree to which occupation modifies such declines is largely unknown.

Methods: Older adults (≥65) completed an online cross-sectional survey containing a standardized hand function questionnaire, occupation-related questions, and demographic information. Participants were then categorized by their longest-held occupation as Blue Collar or White Collar.

Results: Hand impairments were more common in the Blue Collar group (51.5% versus 28.9%, p < 0.05). Odds ratios indicated that Blue Collar workers were 2.71 times more likely to report hand impairments in older adulthood than White Collar workers.

Conclusions: Our findings demonstrated strong associations between occupation type and hand function, underscoring the importance of implementing hand-specific preventative workplace measures and highlighting the need to consider additional risk factors for hand impairments, including occupation, during routine clinical exams.
Predictors of Ever Using Marijuana among U.S. First Responders
Authors: Testoff Addison C. , Koru-Sengul Tulay , Solle Natasha Schaefer , Vidot Denise C. , Lee David J. , Kobetz Erin N. , Caban-Martinez Alberto J. 
Published: January 2025

ABSTRACT

Objective
Estimate ever using marijuana in a sample of U.S. career first responders.

Methods
Health survey cohort data collected between 2018-2024 from employed and retired U.S. first responders was analyzed for frequency of ever using marijuana. Binary logistic regression models estimated the association between ever using marijuana and first responder demographics, employment status, and risky health behaviors.

Results
Among 1,310 current/retired first responders, 15.3% reported ever using marijuana (80.0% seldom, 18.0% often, and 2.0% always). Currently employed firefighter/paramedic/EMT’s had the highest (45.5%) while Battalion/EMS/Deputy/Fire Chiefs had the lowest use (3.0%). First responders who binge drink (adjusted OR = 1.45;[95%CI:1.03-2.03];p = 0.033), former-smoker (2.42;[1.68-3.47];p < 0.001) or current-smokers (3.61;[1.01-12.93];p = 0.049), and were retired (AOR = 1.85;[1.20-2.87] were significantly more likely to report ever using marijuana.

Conclusion
Ever using marijuana was highest among those rank firefighter/paramedics/EMT and significantly associated with binge drinking, retirement and smoking behaviors.
The association between work-life conflict and mental health - A cohort study
Authors: Thorup Lotte , Sørensen Christine Leonhard Birk , Biering Karin 
Published: January 2025

Abstract

Objective
Mental health problems are increasing worldwide, and research has shown that it can be affected by work-life conflict (WLC). The aim of the present study is to examine the association between WLC and both stress and depressive symptoms in early adulthood.

Methods
A cross-sectional and a 4-year follow-up study was conducted using register data and questionnaire data from The West Jutland Cohort Study (VestLiv), Denmark. 1296 individuals (age 28) were included in 2017, and 679 were included in 2021. Validated scales were used to measure both WLC, stress and depressive symptoms. Linear and logistic regression were performed.

Results
An association was found between WLC and both stress and depressive symptoms in both genders.

Conclusion
It is relevant to consider WLC a contributing factor for mental health of individuals in today’s workforce.
Topical review: Ocular surface abnormalities in neurodegenerative disorders
Authors: Waller Sophie E. , Stockwell Joseph B. , Fung Victor S. C. , Anstey Kaarin J. , Colebatch James G. , Markoulli Maria , Krishnan Arun V. 
Published: January 2025

SIGNIFICANCE
In an aging population, the number of people living with neurodegenerative disease is projected to increase. It is vital to develop reliable, noninvasive biomarkers to detect disease onset and monitor progression, and there is a growing body of research into the ocular surface as a potential source of such biomarkers.

BACKGROUND
This article reviews the potential of in vivo corneal confocal microscopy and tear fluid analysis as tools for biomarker development. Corneal confocal microscopy, traditionally used for studying corneal health, offers high-resolution imaging of corneal nerves and has shown promise for examining systemic diseases such as Alzheimer disease and Parkinson's disease. Complementarily, tear fluid analysis, known for its ease of collection, reflects systemic changes in neurodegenerative conditions.

CONCLUSION
Together, these noninvasive techniques provide insights into disease onset and progression and hold potential for advancing diagnostic and treatment strategies.
Health-related Quality of Life and Exercise Capacity in Double Lung Transplant Recipients With Baseline Lung Allograft Dysfunction
Authors: Rullay Alisha , Kaur Karina , Holman Jennifer , van den Bosch Laura C. , Weinkauf Justin G. , Nagendran Jayan , Varughese Rhea A. , Hirji Alim S. , Lien Dale C. , Weatherald Jason C. , Halloran Kieran M. 
Published: January 2025

Background.
Baseline lung allograft dysfunction (BLAD) after lung transplant is associated with an increased risk of dying, but the association with health-related quality of life (HRQL) and exercise capacity is not known. We hypothesized that BLAD would be associated with reduced HRQL and 6-min walk distance (6MWD) at 1 y post–lung transplant.

Methods.
We analyzed patients who underwent lung transplants in our program from 2004 to 2018 who completed 1-y 36-item Short Form (SF-36) questionnaire and 6MWD testing. We secondarily analyzed the Beck Depression Inventory and Borg dyspnea scores in patients using the available data. We defined BLAD as a failure of both forced expiratory volume in 1 s and forced vital capacity to reach ≥80% predicted of a healthy reference population’s lung function on 2 consecutive tests ≥3 wk apart at any time point posttransplant. We tested the relationship between BLAD status and SF-36 physical component summaries and 6MWD using least squares regression, adjusting for age at transplant, sex at birth, and primary lung disease.

Results.
Two hundred sixty-four patients were included, 96 (36%) of whom met the criteria for BLAD. Patients with interstitial lung disease as an indication for transplant and those who received older, female, and heavy smoking donors were at increased risk of BLAD. SF-36 physical component summary scores were lower in patients with BLAD (75 versus 85; P = 0.0076), as were 6MWD values (528 versus 572 m; P = 0.0053). BLAD was associated with lower SF-36 scores (P = 0.0025) and 6MWD (P = 0.0008) in adjusted regression models at 1 y posttransplant. We did not observe differences in Beck Depression Inventory or Borg scores.

Conclusions.
BLAD was associated with reduced HRQL and 6MWD scores at 1 y posttransplant in adjusted models. This suggests that poor posttransplant lung function could contribute to lower HRQL and exercise capacity in lung recipients and is worthy of further exploration in terms of causes, prevention, and treatment.
Impact of Granulocyte Colony-Stimulating Factor (G-CSF) on Clinical Outcomes in Allogeneic Hematopoietic Cell Transplantation: Does Speeding Up Neutrophil Engraftment Make a Difference?
Authors: Alnughmush Ahmed , Sayyed Ayman , Remberger Mats , AL-Shaibani Eshrak , Chen Carol , Chiarello Caden , Pasic Ivan , Novitzky-Basso Igor , Law Arjun Datt , Lam Wilson , Kim Dennis (Dong Hwan) , Gerbitz Armin , Viswabandya Auro , Kumar Rajat , Michelis Fotios V. , Mattsson Jonas 
Published: January 2025

Background.
Despite decades of post–allogeneic hematopoietic cell transplantation (HCT) growth factor utilization, its role remains undefined, leading to ongoing debates and research. The theoretical impacts of growth factors have been challenged in numerous studies.

Methods.
In this retrospective cohort study conducted at the Princess Margaret Cancer Centre, we analyzed the clinical outcomes of 509 patients who underwent allogeneic HCT between May 1, 2019, and May 31, 2022. This study aimed to assess the impact of granulocyte colony-stimulating factor (G-CSF) administration posttransplantation on neutrophil and platelet engraftment, incidence of bloodstream infections (BSIs), graft-versus-host disease, engraftment syndrome (ES), and survival metrics including overall survival, nonrelapse mortality, and graft-versus-host disease–free/relapse-free survival.

Results.
Our findings indicate that G-CSF administration expedited neutrophil engraftment (16 versus 18 d, P = 0.009) and was associated with a decreased incidence of BSI (9.4% versus 31.3%, P = 0.014). However, this benefit was counterbalanced by a significant delay in platelet engraftment (21 versus 17 d, P < 0.001). Multivariate logistic regression analysis identified mismatched donors (odds ratio, 1.72; 95% confidence interval, 1.03-2.88; P = 0.038) and the duration of G-CSF therapy (odds ratio, 1.04; 95% confidence interval, 1.00-1.09; P = 0.038) as independent predictors for the development of ES. Despite these hematological impacts, there was no observed advantage in overall survival, nonrelapse mortality, or graft-versus-host disease–free/relapse-free survival among patients who received G-CSF compared with those who did not.

Conclusions.
Although G-CSF post-HCT expedited neutrophil engraftment and reduced BSI risk, it did not result in a survival advantage. The association with ES necessitates careful consideration.
Autoimmune Encephalitis in Catatonic and Treatment-Resistant Psychotic Patients Referred to Electroconvulsive Therapy
Authors: Beatino Maria Francesca , Weiss Francesco , Torrigiani Samuele , Caruso Valerio , Elefante Camilla , Medda Pierpaolo , Brancati Giulio Emilio , Perugi Giulio 
Published: January 2025

Abstract
Autoimmune encephalitis (AE) tends to manifest as a mixture of neuropsychiatric and somatic symptoms, either of which may predominate, and often shows a progressive clinical course sometimes leading to life-threatening conditions. Catatonic and psychotic syndromes, regardless of whether associated with dysautonomia, are common manifestations of AE, especially concerning the anti-NMDAR subtype. Several autoantibodies targeting different neuronal epitopes have been linked to specific clinical manifestations and their detection is embedded in some of the diagnostic criteria for AE. Therapeutical management of AE is challenged by limited diagnostic abilities and poor understanding of the underlying pathophysiology for most of its subtypes. Although the prompt delivery of disease-modifying therapies represents the cornerstone of treatment and primarily affects prognosis, less is known about the role of symptom specific supportive measures like electroconvulsive therapy (ECT). Based on a systematic review of 26 patient-level descriptions of individuals, each with a diagnosis of AE treated with ECT, a favorable clinical response was found in more than ¾ of the revised cases (76.9%). The most common indications for ECT administration were catatonic and psychotic syndromes, often nonresponsive to prior pharmacotherapy with benzodiazepines, antipsychotic, and other psychotropic drugs. Noteworthy side effects were only reported for 3 of 26 patients. Though the low number of cases and publication bias should be considered as major limitations, current available reports are in support of the inclusion of ECT in the integrated therapeutic algorithm of AE to address psychiatric conditions such as severe psychosis and catatonia.
Use of Electroconvulsive Therapy Treatment in Adolescents in Singapore
Authors: Chen Birong , Fu Yuqian , Li Ziqiang , Tan Xiaowei , Li Juanjuan , Herrera Manoza Michaela , Koh Doris Sok Hian , Tor Phern Chern 
Published: January 2025

Background
Electroconvulsive therapy (ECT) is a highly effective treatment for schizophrenia and mood disorders; however, most evidence is derived from the adult population, with less evidence in adolescents. We sought to determine the use of ECT in adolescents in the Institute of Mental Health (IMH) and evaluate the treatment outcome.

Methods
We conducted a retrospective naturalistic analysis of ECT registry data of patients aged from 10 to 19 years from March 2017 to March 2023. Descriptive analysis was used to analyze the demographics and clinical characteristics. Paired t tests were used to compare the change in clinical outcome scores, including the Brief Psychiatric Rating Scale (BPRS), Montgomery-Asberg Depression Rating Scale (MADRS), Clinical Global Impressions Scale — Severity (CGI-S), and Montreal Cognitive Assessment (MoCA) before and after 2 weeks of ECT treatment.

Results
Fifty-five patients were included for analysis. There was a significant improvement in BPRS (P < 0.001), MADRS (P = 0.005), and CGI-S (P < 0.001), and the average CGI-I score was 2.275 (SD, 0.81), which is equivalent to “much improved” after 6 sessions of treatment. Of all patients, 48.5% showed significant clinical improvement. There was no significant change in MoCA scores (P = 0.218).

Conclusions
Our preliminary findings show that ECT is a safe, rapid, and effective treatment for psychotic and mood disorders in adolescents. Further studies with a larger sample size and specific subgroup analysis are needed to establish the effectiveness of ECT and identify predictors of response in this population.
ZapA employs a two-pronged mechanism to facilitate Z ring formation inEscherichia coli
Authors: Cui Yuanyuan , Gong Han , Yan Di , Li Hao , Yang Wenjie , Li Ying , Chen Xiangdong , Lutkenhaus Joe , Huang Sheng-You , Yang Xinxing , Du Shishen 
Published: January 2025

AbstractThe tubulin-like protein FtsZ assembles into the Z ring which leads to the assembly and activation of the division machinery in most bacteria. ZapA, a widely conserved protein that interacts with FtsZ, plays a pivotal role in organizing FtsZ filaments into a coherent Z ring. Previous studies revealed that ZapA forms a dumbbell-like tetramer that binds cooperatively to FtsZ filaments and aligns them in parallel, leading to the straightening and organization of FtsZ filament bundles. However, how ZapA interacts with FtsZ remains obscure. In this study, we uncover how ZapA interacts with FtsZ to facilitate Z ring formation inEscherichia coli. We find that mutations affecting surface exposed residues at the junction between adjacent FtsZ subunits in a filament as well as in an N-terminal motif of FtsZ weaken its interaction with ZapAin vivoandin vitro, indicating that ZapA binds to these regions of FtsZ. Consistent with this, ZapA prefers FtsZ polymers over monomeric FtsZ molecules and site-specific crosslinking confirmed that the dimer head domain of ZapA is in contact with the junction of FtsZ subunits. As a result, disruption of the putative interaction interfaces between FtsZ and ZapA abolishes the midcell localization of ZapA. Taken together, our results suggest that ZapA tetramers grab the N-terminal tails of FtsZ and then bind to the junctions between FtsZ subunits in the filament to straighten and crosslink parallel FtsZ filaments into the Z ring.SignificanceZapA is a widely conserved FtsZ-associated protein that promotes the organization of the Z ring, the key cytoskeletal element in the bacterial divisome. Although ZapA is known to crosslink FtsZ filaments, how it interacts with FtsZ remains enigmatic. In this study, we find thatE. coliZapA utilizes a dual binding mode in which it binds the junction between FtsZ subunits in a filament and to an N-terminal motif in FtsZ so that it can straighten and crosslink parallel FtsZ filaments simultaneously. Since the junction is formed when FtsZ polymerizes and falls apart when FtsZ depolymerizes, this interaction mode indicates that ZapA employs the polymerization dynamics of FtsZ to organize the filaments into the Z ring.
Massive culture-based approach for the screening of AmpC-, ESBL- and carbapenemase producers from complex samples
Authors: Taddeucci-Rocha Gabriel , de Oliveira Costa Victoria , da Silva Sarah Vitória Martins , Gonçalves Jéssica Britto , da Silva Natalia Chilinque Zambão , Garnica Marcia Maiolino , Picão Renata Cristina 
Published: January 2025

ABSTRACTGram-negative bacilli producing beta-lactamases are major causes of difficult-to-treat infections, especially the AmpC, ESBL, and carbapenemase types. Their spread within and outside hospital settings demands effective detection and monitoring in various environments, but current methods for this purpose often neglect important groups of beta-lactamases or are expensive and time consuming. We aimed to develop and test a massive culture approach to detect and differentiate between beta-lactamase producers from complex samples. The method involved an enrichment step on MacConkey agar supplemented with ceftriaxone to select for AmpC, ESBL, and carbapenemase producers, followed by replica plating under selective pressures (cefoxitin, cefepime, imipenem) to differentiate them. The massive culture approach effectively differentiated strains producing different beta-lactamases in mixed cultures. In tests with rectal swabs, our method demonstrated 100% sensitivity, higher specificity, and greater accuracy for ESBL detection compared to the reference method. Additionally, it identified a broader spectrum of beta-lactamase producers, including AmpC and carbapenemase. The massive culture approach is a promising tool for detecting and differentiating gram-negative bacilli producing beta-lactamases from rectal swabs. Due to the additional time required to produce results, this method is most suitable for central and research laboratories and enhances surveillance capabilities for antimicrobial resistance.IMPORTANCEThe dissemination of multidrug-resistant bacteria producing beta-lactamases in healthcare and community settings demands effective detection and monitoring. Existing methods may miss key beta-lactamase groups or are costly and time-consuming. In this study, a comprehensive culture-based approach using replica plating was developed and used to detect and differentiate beta-lactamase producers from rectal swabs. It showed higher specificity and greater accuracy for ESBL detection compared to the reference method, while identifying a broader range of beta-lactamase producers, including AmpC and carbapenemase. Despite requiring additional processing time, this method is suitable for central and research laboratories, enhancing antimicrobial resistance surveillance.
The subunit 3 of the SUPERKILLER (SKI) complex mediates miR172-directed cleavage ofNodule Number Control 1(NNC1) to modulate nodulation inMedicagotruncatula
Authors: Traubenik Soledad , Reynoso Mauricio Alberto , Sánchez-Rodríguez Francisco , Yacullo Milagros , Christ Aurelie , Hummel Maureen , Blein Thomas , Crespi Martín , Bailey-Serres Julia , Blanco Flavio Antonio , Zanetti María Eugenia 
Published: January 2025

AbstractLegumes and rhizobia establish a nitrogen-fixing symbiosis that involves the formation of a lateral root organ, the nodule, and the infection process that allows intracellular accommodation of rhizobia within nodule cells. This process involves significant gene expression changes regulated at the transcriptional and post-transcriptional levels. We have previously shown that a transcript encoding the subunit 3 of the Superkiller Complex (SKI), which guides mRNAs to the exosome for 3′-to-5′ degradation, is required for nodule formation and bacterial persistence within the nodule, as well as the induction of early nodulation genes (e.g.,MtENOD40) during theMedicago truncatula-Sinorhizobium melilotisymbiosis. Here, we reveal through transcript degradome and small RNA sequencing analysis that knockdown ofMtSKI3impairs the miR172-directed endonucleolytic cleavage of the mRNA encoding Nodule Number Control 1 (MtNNC1), an APETALA2 transcription factor that negatively modulates nodulation. Knockdown ofMtNNC1enhances nodule number, bacterial infection, and the induction ofMtENOD40upon inoculation withS. melilotiwhereas overexpression of a miR172-resistant form ofMtNNC1significantly reduces nodule formation. This work identifies miR172 cleavage ofMtNNC1and its control by MtSKI3, a component of the 3′-to-5′mRNA degradation pathway, as a new regulatory hub controlling indeterminate nodulation.