bluuebunny/crossref_metadata_2025_split huggingface dataset:
Understanding caregiver burden with accessing sickle cell care in the Midwest and their perspective on telemedicine
Authors: Jacob Seethal A. , Bouck Jillian , Daas Roua , Jackson Meghan Drayton , LaMotte Julia E. , Carroll Aaron E. 
Published: May 2023

Abstract
Background
Survival for children with sickle cell disease (SCD) has improved significantly. However, patients with SCD still encounter several impediments to accessing adequate healthcare. Rural and medically underserved areas, such as parts of the Midwest, can exacerbate these barriers, separating children with SCD from subspecialists even further. Telemedicine has been a means to close these gaps in care for children with other special healthcare needs, but few studies have discussed how caregivers of children with SCD perceive its use.

Methods
The objective of this study is to understand the experiences of caregivers of pediatric SCD patients in a geographically diverse area in the Midwest in accessing care, and their perspectives of telemedicine. Caregivers of children with SCD completed an 88-item survey via a secured REDCap link either in-person or via secure text. Descriptive statistics (means, medians, ranges, frequencies) were performed for all responses. Univariate chi square tests were performed to analyze associations, particularly with telemedicine responses.

Results
The survey was completed by 101 caregivers. Nearly 20% of families traveled more than 1 hour to reach the comprehensive SCD center. Other than their SCD provider, caregivers reported their child having at least 2 other healthcare providers. Most barriers caregivers identified were financial or resource based. Almost a quarter of caregivers expressed feeling as though these barriers impacted their and/or their child’s mental health. Ease of access to team members, as well as scheduling, were common facilitators of care cited by caregivers. The majority were willing to participate in telemedicine visits, regardless of how far they lived from the SCD center, though many noted aspects requiring adaptation.

Conclusion
This cross-sectional study describes barriers to care experienced by caregivers of children with SCD, regardless of proximity to an SCD center, as well as caregiver perceptions of the usefulness and acceptability of telemedicine for SCD care.
Effects of care of multimorbid patients in general practices by advanced practice nurses (FAMOUS): study protocol for a nonrandomized controlled trial
Authors: Stemmer Renate , Büchler Britta , Büttner Matthias , Dera-Ströhm Christina , Klein Joachim , Gianicolo Emilio 
Published: May 2023

Abstract
Background
Multimorbidity is a common phenomenon among patients treated in general practices. Key challenges within this group include functional difficulties, polypharmacy, treatment burden, fragmentation of care, reduced quality of life and increased health care utilization. These problems cannot be solved in the short consultation time of a general practitioner (GP) since there is an increasing shortage of GPs. In many countries, advanced practice nurses (APNs) are successfully integrated into primary health care for multimorbid patients.
The objective of this study is to examine whether the integration of APNs in the primary care of multimorbid patients in Germany leads to optimized care of the target group and to a reduction in the workload of the GPs.

Methods
The intervention includes the integration of APNs into the care for multimorbid patients in general practice for twelve months. Qualifications for APNs include a master's level academic degree and 500 hours of project-specific training. Their tasks include in-depth assessment, preparation, implementation, monitoring and evaluation of a person-centred and evidence-based care plan.
In this nonrandomized controlled trial, a prospective multicentre mixed methods study will be performed. The main inclusion criterion was the cooccurrence of three chronic diseases. For data collection in the intervention group (n = 817), routine data from health insurance companies and association of statutory health insurance physicians (ASHIP) will be used, as well as qualitative interviews. In addition, the intervention will be assessed through documentation of the care process and standardized questionnaires using a longitudinal design. The control group (n = 1634) will receive standard care. For the evaluation, routine data from health insurance companies are matched at a ratio of 1:2.
Outcomes will be measured using emergency contacts and GP visits, treatment costs, health status of the patients and the satisfaction of parties involved.
The statistical analyses will include Poisson regression to compare outcomes between the intervention and control groups. Descriptive and analytical statistical methods will be used in the longitudinal analysis of the intervention group data. Cost analysis will compare total costs and subgroup costs between the intervention and control groups. Qualitative data will be analysed using content analysis.

Discussion
Challenges to this protocol could include the political and strategic environment as well as the planned number of participants.

Trial registration
DRKS00026172 on DRKS.
Alterations in the microenvironment and the effects produced of TRPV5 in osteoporosis
Authors: Luo Zhi-heng , Ma Jian-xiong , Zhang Wei , Tian Ai-xian , Gong Shu-wei , Li Yan , Lai Yu-xiao , Ma Xin-long 
Published: May 2023

AbstractThe pathogenesis of osteoporosis involves multiple factors, among which alterations in the bone microenvironment play a crucial role in disrupting normal bone metabolic balance. Transient receptor potential vanilloid 5 (TRPV5), a member of the TRPV family, is an essential determinant of the bone microenvironment, acting at multiple levels to influence its properties. TRPV5 exerts a pivotal influence on bone through the regulation of calcium reabsorption and transportation while also responding to steroid hormones and agonists. Although the metabolic consequences of osteoporosis, such as loss of bone calcium, reduced mineralization capacity, and active osteoclasts, have received significant attention, this review focuses on the changes in the osteoporotic microenvironment and the specific effects of TRPV5 at various levels.
Pulmonary adenoid cystic carcinoma: molecular characteristics and literature review
Authors: Chen Zhixin , Jiang Jiapeng , Fan Ying , Lu Hongyang 
Published: May 2023

Abstract
Background
Pulmonary adenoid cystic carcinoma (PACC) is an exceptionally rare salivary gland-type malignant neoplasm. Because of its clinical manifestations, imaging features are not different from other types of non-small cell lung cancer, which is a diagnostic challenge for most doctors.

Conclusions
A review of the literature shows that high amounts of immunohistochemical (IHC) markers, such as CK7, CD117, P63, SMA, CK5/6, and S-100 are helpful for PACC diagnosis. Surgical resection is the main treatment of PACC, but treatment options for advanced PACC patients are limited and the research of molecular targeted drugs is ongoing in advanced cases not eligible for surgery. Currently, research on PACC targeted therapy mainly focuses on the exploration of v-myb avian myeloblastosis virus oncogene homolog (MYB) and its downstream target genes. In addition, median tumor mutation burden and PD-1/PD-L1 were lower in PACC, which may indicate poor efficacy of immunotherapy in PACC patients. This review focuses on the pathologic features, molecular characteristics, diagnosis, treatment and prognosis of PACC to establish a comprehensive understanding of PACC.
Optimization of in-brace corrective force in adolescents with Lenke type 5 curve using finite element model
Authors: Li Kepeng , Wu Jincheng , Yang Dongmei , Xu Hanpeng , Wen Wangqiang , Xu Haoxiang , Ma Guoju , Han Ye 
Published: May 2023

Abstract
Background
Pelvic parameters have been taken into consideration for the evaluation of the outcomes of bracing in AIS. To discuss the stress required to correct the pelvic deformity related to Lenke5 adolescent idiopathic scoliosis (AIS) by finite element analysis, and provide a reference for the shaping of the pelvic region of the brace.

Methods
An three-dimensional (3D) corrective force on the pelvic area was defined. Computed tomography images were used to reconstruct a 3D model of Lenke5 AIS. Computer-aided engineering software Abaqus was used to implement finite element analysis. By adjusting the magnitude and position of corrective forces, coronal pelvic coronal plane rotation (PCPR) and Cobb angle (CA) of lumbar curve in the coronal plane, horizontal pelvic axial plane rotation, and apical vertebra rotation (AVR) were minimized to achieve the best effect on the spine and pelvic deformity correction. The proposed corrective conditions were divided into three groups: (1) forces applied on X-axis; (2) forces applied both in the X- and Y-axis; and (3) forces applied along the X-, Y-, and Z-axis at the same time.

Results
In three groups, CA correction reduced by 31.5%, 42.5%, and 59.8%, and the PCPR changed to 12°, 13°, and 1° from 6.5°, respectively. The best groups of correction forces should simultaneously locate on the sagittal, transverse, and coronal planes of the pelvis.

Conclusions
For Lenke5 AIS, 3D correction forces can sufficiently reduce scoliosis and pelvic asymmetrical state. Force applied along the Z-axis is vital to correct the pelvic coronal pelvic tilt associated with Lenke5 AIS.
RabbitTClust: enabling fast clustering analysis of millions of bacteria genomes with MinHash sketches
Authors: Xu Xiaoming , Yin Zekun , Yan Lifeng , Zhang Hao , Xu Borui , Wei Yanjie , Niu Beifang , Schmidt Bertil , Liu Weiguo 
Published: May 2023

AbstractWe present RabbitTClust, a fast and memory-efficient genome clustering tool based on sketch-based distance estimation. Our approach enables efficient processing of large-scale datasets by combining dimensionality reduction techniques with streaming and parallelization on modern multi-core platforms. 113,674 complete bacterial genome sequences from RefSeq, 455 GB in FASTA format, can be clustered within less than 6 min and 1,009,738 GenBank assembled bacterial genomes, 4.0 TB in FASTA format, within only 34 min on a 128-core workstation. Our results further identify 1269 redundant genomes, with identical nucleotide content, in the RefSeq bacterial genomes database.
The influence of the environment on the patient-centered therapeutic relationship in physical therapy: a qualitative study
Authors: Morera-Balaguer Jaume , Martínez-González Mª Carmen , Río-Medina Sonia , Zamora-Conesa Víctor , Leal-Clavel Marina , Botella-Rico José Martín , Leirós-Rodríguez Raquel , Rodríguez-Nogueira Óscar 
Published: May 2023

Abstract
Background
Currently, in the scientific literature there is a great interest on the study of strategies to implement patient-centered care. One of the main tools for this is the therapeutic relationship. Some studies suggest that the perception of the environment in which the treatment takes place can influence the perception of its quality, but this is not explored in physical therapy. For all these reasons, the aim of this study was to understand the influence of the environment in which physical therapy treatment takes place on the patients’ perception of the quality of the patient-centered therapeutic relationship in public health centers in Spain.

Methods
A qualitative study analysed thematically using a modified grounded theory approach. Data collection used semistructured interviewing during focus groups.

Results
We conducted four focus groups. The size of the focus groups ranged from six to nine participants. In total, 31 patients participated in these focus groups. Participants described a series of specific experiences and perceptions relating to the environment, which they felt were influential in the establishment of therapeutic patient-centered relationships, including six physical factors (Architectural barriers, Furniture, Use of the computer, Physical space, Ambiet conditions, and Privacy) and six organizational factors (Patient-physical therapist ratio, Treatment interruptions, Social factors, Continuity with the professional, Lack of professional autonomy, and Coordination or communication among team members).

Conclusion
The results of this study highlight environmental factors that affect the quality of the therapeutic patient-centered relationship in physical therapy from the patient’s point of view, and emphasize the need for physical therapists and administrators to underline the need to review these factors and take them into account in their service delivery.
Decoupling effect and driving factors of carbon footprint in megacity Wuhan, Central China
Authors: Pan Gao , Li Xinhang , Pan Deng , Liu Wensheng 
Published: May 2023

Abstract
Background
China’s 35 largest cities, including Wuhan, are inhabited by approximately 18% of the Chinese population, and account for 40% energy consumption and greenhouse gas emissions. Wuhan is the only sub-provincial city in Central China and, as the eighth largest economy nationwide, has experienced a notable increase in energy consumption. However, major knowledge gaps exist in understanding the nexus of economic development and carbon footprint and their drivers in Wuhan.

Methods
We studied Wuhan for the evolutionary characteristics of its carbon footprint (CF), the decoupling relationship between economic development and CF, and the essential drivers of CF. Based on the CF model, we quantified the dynamic trends of CF, carbon carrying capacity, carbon deficit, and carbon deficit pressure index from 2001 to 2020. We also adopted a decoupling model to clarify the coupled dynamics among total CF, its accounts, and economic development. We used the partial least squares method to analyze the influencing factors of Wuhan’s CF and determine the main drivers.

Results
The CF of Wuhan increased from 36.01 million t CO2eq in 2001 to 70.07 million t CO2eq in 2020, a growth rate of 94.61%, which was much faster than that of the carbon carrying capacity. The energy consumption account (84.15%) far exceeded other accounts, and was mostly contributed by raw coal, coke, and crude oil. The carbon deficit pressure index fluctuated in the range of 8.44–6.74%, indicating that Wuhan was in the relief zone and the mild enhancement zone during 2001–2020. Around the same time, Wuhan was in a transition stage between weak and strong CF decoupling and economic growth. The main driving factor of CF growth was the urban per capita residential building area, while energy consumption per unit of GDP was responsible for the CF decline.

Conclusions
Our research highlights the interaction of urban ecological and economic systems, and that Wuhan’s CF changes were mainly affected by four factors: city size, economic development, social consumption, and technological progress. The findings are of realistic significance in promoting low-carbon urban development and improving the city’s sustainability, and the related policies can offer an excellent benchmark for other cities with similar challenges.
Appropriate antibiotic use and antimicrobial resistance: knowledge, attitudes and behaviour of medical students and their needs and preferences for learning
Authors: Wiese-Posselt Miriam , Lâm Thiên-Trí , Schröder Christin , Schneider Sandra , Kurzai Oliver , Feufel Markus A. , Gastmeier Petra 
Published: May 2023

Abstract
Background
The impact of an appropriate use of antibiotics on the prevention of antimicrobial resistance (AMR) has been demonstrated. Surveys have shown, however, that medical students do not feel sufficiently trained to use antibiotics wisely. The aims of our study were (1) to describe what medical students currently know about appropriate antibiotic use, and (2) to identify students’ learning preferences as a basis for developing student-centred teaching modules to convey the basics of AMR prevention.

Methods
We performed an online survey at Charité Universitätsmedizin Berlin and the Julius-Maximilians-University Würzburg on the knowledge, attitudes, and behaviour (KAB) of medical students concerning AMR, antibiotic treatment options, and their perceptions of AMR topics addressed in the medical curriculum. Participants were able to fill out an online questionnaire between December 2019 and February 2020. In addition, we conducted focus group discussions with lecturers and medical students in winter 2019/2020 to identify AMR-related learning needs and preferences. Data were analysed descriptively.

Results
Overall, 356 students (response rate 5.1%) participated in the KAB survey. Of these, 192 (54%) strongly agreed that the topic of AMR is relevant to students’ clinical practice and 48% (171/355) stated that their future antibiotic prescription behaviour will have an influence on AMR development in their region. Participating students seemed to be interested in the topic of AMR and antibiotic therapy. But even of them, only 46% answered the question about the length of antibiotic use for community-acquired pneumonia correctly and 57% the question about the appropriate use of antibiotics in Staphylococcus aureus infections. Focus group discussions with students (n = 7) and lecturers (n = 9) identified a lack of competence in the responsible use of antibiotics and the prevention of AMR. Respondents stated that the teaching formats and AMR-related content should emphasize clinical applications, interaction with peers/clinicians, and repeated formative feedback from instructors.

Conclusions
Our results show that even medical students who were interested in the AMR problem were not able to use antibiotics appropriately due to gaps in knowledge and a lack of clinical skills. Based on the insights gained in the learning preferences of students and their content priorities, improved student-centred teaching materials should be developed.
Activation of a hypothalamus-habenula circuit by mechanical stimulation inhibits cocaine addiction-like behaviors
Authors: Jang Han Byeol , Ahn DanBi , Chang Suchan , Kim Hyung Kyu , Lee Bong Hyo , Kim Sang Chan , Steffensen Scott C. , Bills Kyle B. , Lee Hubert , Kim Hee Young 
Published: May 2023

Abstract
Background
Mechanoreceptor activation modulates GABA neuron firing and dopamine (DA) release in the mesolimbic DA system, an area implicated in reward and substance abuse. The lateral habenula (LHb), the lateral hypothalamus (LH), and the mesolimbic DA system are not only reciprocally connected, but also involved in drug reward. We explored the effects of mechanical stimulation (MS) on cocaine addiction-like behaviors and the role of the LH-LHb circuit in the MS effects. MS was performed over ulnar nerve and the effects were evaluated by using drug seeking behaviors, optogenetics, chemogenetics, electrophysiology and immunohistochemistry.

Results
Mechanical stimulation attenuated locomotor activity in a nerve-dependent manner and 50-kHz ultrasonic vocalizations (USVs) and DA release in nucleus accumbens (NAc) following cocaine injection. The MS effects were ablated by electrolytic lesion or optogenetic inhibition of LHb. Optogenetic activation of LHb suppressed cocaine-enhanced 50 kHz USVs and locomotion. MS reversed cocaine suppression of neuronal activity of LHb. MS also inhibited cocaine-primed reinstatement of drug-seeking behavior, which was blocked by chemogenetic inhibition of an LH-LHb circuit.

Conclusion
These findings suggest that peripheral mechanical stimulation activates LH-LHb pathways to attenuate cocaine-induced psychomotor responses and seeking behaviors.
Natural occurrence of Beauveria bassiana (Ascomycota: Hypocreales) infecting Spodoptera frugiperda (J. E. Smith) (Lepidoptera: Noctuidae) and earwig in eastern DR Congo
Authors: Cokola Marcellin C. , Ben Fekih Ibtissem , Bisimwa Espoir B. , Capparos Megido Rudy , Delvigne Frank , Francis Frédéric 
Published: May 2023

AbstractBackgroundThe fall armyworm (FAW),Spodoptera frugiperda(J. E. Smith) (Lepidoptera: Noctuidae), poses a threat to the food security of populations in sub-Saharan Africa because of its damage to maize crops. As alternative to the use of hazardous pesticides, microbial control is one of the most promising sustainable approaches adopted to limit the damages caused byS. frugiperda. The sampling targeted mainly larvae ofS. frugiperda; however, during the survey, cadavers of earwig found on the same sampling sites were also collected and involved in the study. Cadavers of targeted insects, with and without sign of fungal infection, were sampled from 3 localities in eastern DR Congo. Culture of fungal isolates was performed in selective Sabouraud dextrose agar media.ResultsMorphological study of fungal features such as conidia (shape and size) and conidiophores showed that the isolates were from the genusBeauveria. Conidial measurements were highly variable and ranged from 2.4 to 3.6 µm in length and from 1.75 to 3.0 µm in width. Molecular characterization and phylogenetic analysis of the 2Beauveriaisolates based on DNA sequencing of ITS-5.8S region confirmed that both isolates belong toBeauveria bassiana. The 2 isolates ofB. bassianaP5E (OP419735.1) and KA14 (OP419734.1) were isolated from cadavers of FAW and earwig, respectively. The alignment with different sequences ofB. bassianafrom different continent showed that P5E belonged to the same clade of previous isolates reported from Iran and Mexico, while KA14 was with the same clade as isolates from Kenya and China.ConclusionTo our knowledge, this is the first study reporting the occurrence ofB. bassianainfecting FAW and earwig in eastern DR Congo and in Africa.
Molecular profiling of resistance alleles in Pectinophora gossypiella (Saunders) (Lepidoptera: Gelechiidae) collected from different locations
Authors: Likhitha P. , Naik V. Chinna Babu , Moharil M. P. , Undirwade D. B. , Kulkarni U. S. , Kolhe A. V. 
Published: May 2023

Abstract
Background
After the commercialization of insect-resistant transgenic Bt cotton Bollgard I & II, India ranks first in the world in cotton production. Cotton insecticide consumption was drastically reduced as nearly 95% of the cotton area was replaced with Bollgard II. However, the benefits of transgenic cotton appear to have been diminished as the pink bollworm, Pectinophora gossypiella (Saunders) (Lepidoptera: Gelechiidae) developed field resistance to Cry1Ac expressing Bt cotton in India in 2008. In 2015, there was an unusual survival of P. gossypiella on Bollgard II cotton in some parts of Gujarat and Maharashtra, which is a cause for concern.

Results
In the present study, PCR analysis and gel visualization of BGII resistant field population of P. gossypiella showed that the presence of r1, r2, r3, r1s, r2s, r3s, and ss mutated cadherin alleles, which produced amplicon sizes of 750 bp, 700 bp, 730 bp, 12,700 bp, 750 bp, 980 bp, 600 bp and 1600 bp, respectively, when seven different types of cadherin allele(s) specific primers were used. The r1 mutant allele was detected in Amaravati, Wardha, Yavatmal, and Nagpur, but not in Akola and Buldhana, using primers r1disfor and Int-540. The r2 mutant allele was detected in Akola, Wardha, and Nagpur, but not in Buldhana, Amaravati, and Yavatmal, using primers r2disback and Cad2366. The r3 mutant allele was detected in all locations using primers r3disback and Cad3221. Amplicons of sizes 750 bp, 700 bp and 730 bp were obtained for r1, r2, and r3 mutant alleles, respectively. The sizes of the amplicons were 1270 bp for r1s, 750 bp for r2s, and 980 bp for r3s.The absence of bands for r1, r2, and r3 cadherin alleles in individuals indicated the possibility of having the ss allele, which was confirmed using primers Cad3324 and Int-651. The presence of ss mutant allele was observed in field-collected P. gossypiella populations from BG II cotton in various locations, with a 600 bp and 1600 bp size amplicons produced using the same primers.

Conclusions
PCR analysis revealed the presence of r1, r2, r3, r1s, r2s, r3s, and ss mutated cadherin alleles in field-collected populations of Vidarbha which provide evidence to field-evolved resistance of P. gossypiella to BGII cotton.