bluuebunny/crossref_metadata_2025_split huggingface dataset:
Senescent cells deposit intracellular contents through adhesion-dependent fragmentation
Authors: Durik Matej , Gonçalves Daniel Sampaio , Spiegelhalter Coralie , Messaddeq Nadia , Keyes William M. 
Published: January 2023

ABSTRACTCellular senescence is a complex cell state with roles in tumor suppression, embryonic development and wound repair. However, when misregulated, senescence contributes to aging and disease. Here we identify that senescent cells generate/break off large membranebound fragments of themselves through cell-to-cell adhesion. We designate these as senescent-cell adhesion fragments (SCAFs) which were present in all types of senescent cell examined. We show they contain many organelles from the original cell, but without nuclear material. Quantitative and dynamic profiling shows that SCAFs are large, may persist for a number of days, but rupture and release their contents onto neighboring cells. Protein profiling identifies that SCAFs contain a complex proteome including immune recruitment factors and damage-associated molecular patterns (DAMPs). Functional studies reveal that SCAFs activate signatures related to wound healing and cancer, and promote invasion and migration. Altogether, we uncover an additional cellular feature of senescent cells, by which they deposit intracellular contents on other cells. We speculate this may aid in boosting immune responses, but in chronic situations, may contribute to debris buildup, inflammaging and age-associated changes.
High qualityde novogenome assembly of non-conventional yeastKazachstania bulderia new potential low pH production host for biorefineries
Authors: Balarezo-Cisneros L. N. , Timouma S. , Hanak A. , Currin A. , Valle F. , Delneri D 
Published: January 2023

AbstractKazachstania bulderiis a yeast species belonging to a ubiquitous group of non-conventional yeasts which has the ability to grow efficiently on glucose and δ-gluconolactone at low pH. This unique trait makesK. bulderian ideal candidate as a new host for low pH fermentation processes for sustainable production of green chemicals such as organic acids. To accelerate strain development with this species, detailed information of its genetics is needed. Here, by employing high accuracy long read sequencing we report a high-quality phased genome assembly for three strains belonging toK. bulderispecies, including the type strain. The sequences were assembled into 12 chromosomes with a total length of 14Mb, and the genome was fully annotated at structural and functional levels, including allelic and structural variants, ribosomal array, centromeres and mating type locus. This high-quality reference genome provides an essential resource to advance our fundamental knowledge of biotechno-logically relevant non-conventional yeasts and to support the development of genetic tools for manipulating such strains towards their use as production hosts biotechnological processes.
Transcriptional signatures of heroin intake and seeking throughout the brain reward circuit
Authors: Browne Caleb J , Futamura Rita , Minier-Toribio Angélica , Hicks Emily M , Ramakrishnan Aarthi , Martínez-Rivera Freddyson , Estill Molly , Godino Arthur , Parise Eric M , Torres-Berrío Angélica , Cunningham Ashley M , Hamilton Peter J , Walker Deena M , Huckins Laura M. , Hurd Yasmin L , Shen Li , Nestler Eric J 
Published: January 2023

AbstractOpioid use disorder (OUD) looms as one of the most severe medical crises currently facing society. More effective therapeutics for OUD requires in-depth understanding of molecular changes supporting drug-taking and relapse. Recent efforts have helped advance these aims, but studies have been limited in number and scope. Here, we develop a brain reward circuit-wide atlas of opioid-induced transcriptional regulation by combining RNA sequencing (RNAseq) and heroin self-administration in male mice modeling multiple OUD-relevant conditions: acute heroin exposure, chronic heroin intake, context-induced drug-seeking following prolonged abstinence, and heroin-primed drug-seeking (i.e., “relapse”). Bioinformatics analysis of this rich dataset identified numerous patterns of molecular changes, transcriptional regulation, brain-region-specific involvement in various aspects of OUD, and both region-specific and pan-circuit biological domains affected by heroin. Integrating RNAseq data with behavioral outcomes using factor analysis to generate an “addiction index” uncovered novel roles for particular brain regions in promoting addiction-relevant behavior, and implicated multi-regional changes in affected genes and biological processes. Comparisons with RNAseq and genome-wide association studies from humans with OUD reveal convergent molecular regulation that are implicated in drug-taking and relapse, and point to novel gene candidates with high therapeutic potential for OUD. These results outline broad molecular reprogramming that may directly promote the development and maintenance of OUD, and provide a foundational resource to the field for future research into OUD mechanisms and treatment strategies.
Trophoblast organoids with physiological polarity model placental structure and function
Authors: Yang Liheng , Liang Pengfei , Yang Huanghe , Coyne Carolyn B. 
Published: January 2023

ABSTRACTHuman trophoblast organoids (TOs) are a three-dimensionalex vivoculture model that can be used to study various aspects of placental development, physiology, and pathology. Previously, we showed that TOs derived from full-term human placental tissue could be used as models of trophoblast innate immune signaling and teratogenic virus infections. Here, we developed a method to culture TOs under conditions that recapitulate the cellular orientation of chorionic villiin vivo, with the multi-nucleated syncytiotrophoblast (STB) localized to the outer surface of organoids and the proliferative cytotrophoblasts (CTBs) located on the inner surface. We show that standard TOs containing the STB layer inside the organoid (STBin) develop into organoids containing the STB on the outer surface (STBout) when cultured in suspension with gentle agitation. STBoutorganoids secrete higher levels of select STB-associated hormones and cytokines, including human chorionic gonadotropin (hCG) and interferon (IFN)-λ2. Using membrane capacitance measurements, we also show that the outermost surface of STBoutorganoids contain large syncytia comprised of >50 nuclei compared to STBinorganoids that contain small syncytia (<10 nuclei) and mononuclear cells. The growth of TOs under conditions that mimic the cellular orientation of chorionic villiin vivothus allows for the study of a variety of aspects of placental biology under physiological conditions.
The psychosis risk factorRBM12encodes a novel repressor of GPCR/cAMP signal transduction
Authors: Semesta Khairunnisa M. , Garces Angelica , Tsvetanova Nikoleta G. 
Published: January 2023

AbstractRBM12is a high-penetrance risk factor for familial schizophrenia and psychosis, yet its precise cellular functions and the pathways to which it belongs are not known. We utilize two complementary models, HEK293 cells and human iPSC-derived neurons, and delineate RBM12 as a novel repressor of the G protein-coupled receptor/cyclic AMP/protein kinase A (GPCR/cAMP/PKA) signaling axis. We establish that loss of RBM12 leads to hyperactive cAMP production and increased PKA activity as well as altered neuronal transcriptional responses to GPCR stimulation. Notably, the cAMP and transcriptional signaling steps are subject to discrete RBM12-dependent regulation. We further demonstrate that the twoRBM12truncating variants linked to familial psychosis impact this interplay, as the mutants fail to rescue GPCR/cAMP signaling hyperactivity in cells depleted of RBM12. Lastly, we present a mechanism underlying the impaired signaling phenotypes. In agreement with its activity as an RNA-binding protein, loss of RBM12 leads to altered gene expression, including that of multiple effectors of established significance within the receptor pathway. Specifically, the abundance of adenylyl cyclases, phosphodiesterase isoforms, and PKA regulatory and catalytic subunits is impacted by RBM12 depletion. We note that these expression changes are fully consistent with the entire gamut of hyperactive signaling outputs. In summary, the current study identifies a previously unappreciated role for RBM12 in the context of the GPCR/cAMP pathway that could be explored further as a tentative molecular mechanism underlying the functions of this factor in neuronal physiology and pathophysiology.
Effect of innovation practices of banks on customer loyalty: an SEM-ANN approach
Authors: Tiwari Pankaj 
Published: January 2023

PurposeThe purpose of this study is to examine the effects of banking innovations (INNs) on customer experience (EXP), satisfaction (SAT) and loyalty (LOY).Design/methodology/approachThe author evaluated the data using a structural equation method-artificial neural network (SEM-ANN) method. The author’s results show the presence of relationship between INN, EXP, SAT and LOY. In this study, the node layers of ANNs add an input layer, hidden layers and an output layer. Each “node” acts as an artificial neuron that communicates with others. The ANN model takes the variables from the SEM analysis as input neurons.FindingsThe author observed the significant effects between INN, EXP, SAT and LOY using the normalised importance generated by the multilayer perceptron used in the feed-forward back propagation of the ANN methodology. In this study, the ANN model can predict LOY through service innovation, with a forecast accuracy of 77.6%.Originality/valueBy applying neural network modelling, this research helps us understand how service innovation affects customer behaviour. For the first time, the author examined service innovations' direct and indirect impact on loyalty through EXP and SAT. The author made a significant conceptual contribution by using a non-compensatory model of ANNs to circumvent the limitations of linear models.
Does board diversity affect the cost of debt financing? Empirical evidence from Turkey
Authors: Aksoy Mine , Yilmaz Mustafa Kemal 
Published: January 2023

Purpose
This study aims to investigate the effects of board characteristics on the cost of debt for non-financial companies in the Turkish capital markets.

Design/methodology/approach
Using a sample of 211 non-financial companies listed on Borsa Istanbul, this study examines how chairperson gender and board characteristics affect the cost of debt by using panel data analysis over the period of 2016–2020. A system generalized method of moments model is also applied to test the endogeneity issue.

Findings
The findings show that the presence of female chairperson and female directors on board reduces the cost of debt and the perceptions of default risk by fund providers, while board independence and board size do not have a significant impact on the cost of debt. The results provide insightful information for companies and policymakers. Companies can alter board composition through gender diversity, while policymakers can introduce new policies in encouraging the presence of female directors on boards.

Originality/value
This study primarily enriches the literature on the effect of board diversity on debt financing cost in a leading emerging market, enabling companies in emerging markets to better mitigate agency costs and finance their investment through effective board composition. Second, it provides evidence that financial institutions consider companies with chairwomen and women directors on the boards less risky and charge them less for debt financing than they do for companies with man chairperson. Finally, the results support policymakers to take actions to increase female presence on board.
Determinants of ESG disclosure among listed firms under voluntary and mandatory ESG disclosure regimes in Hong Kong
Authors: Chung Ricky , Bayne Lyndie , Birt Jacqueline Louise 
Published: January 2023

PurposeThe authors examine the determinants of ESG disclosure and differentiate between voluntary and mandatory disclosure regimes in Hong Kong.Design/methodology/approachThe authors analyse both Bloomberg ESG scores and a disclosure index score, manually constructed according to the 2019 Hong Kong Exchange ESG Guide using regression tests.FindingsThe results indicate that the level of concentrated ownership is negatively associated with the quantity of ESG disclosure only in the voluntary disclosure period, suggesting that agency problems are alleviated when ESG reporting is mandatory. The findings also show that larger firms significantly disclose higher levels of ESG information in both voluntary and mandatory disclosure periods. Furthermore, the extent of ESG disclosure significantly increases when firms' sustainability reports are audited by Big 4 accounting firms only in the voluntary disclosure period. Finally, the control variables are significantly related to the level of ESG disclosure showing that ESG disclosure increased over time and is significantly different among industries.OriginalityThe authors make contributions to the literature on non-financial disclosure in relation to ESG reporting by examining the relationship between firm characteristics and ESG disclosure in the Hong Kong context under both voluntary and mandatory disclosure regimes. This study also provides important implications for other stock markets and relevant stakeholders including preparers, users and the sustainability profession.
Work engagement and job performance among nurses in the public healthcare sector in the United Arab Emirates
Authors: Al Badi Faitma Mohammed , Cherian Jacob , Farouk Sherine , Al Nahyan Moza 
Published: January 2023

Purpose
Nurses who are more engaged in their work, and have the right job characteristics and positive organizational factors, are expected to perform better. The purpose of this study is to improve the performance in the healthcare sector in the United Arab Emirates (UAE), thus this study explored the job characteristics and organizational factors that affect work engagement and job performance of nurses.

Design/methodology/approach
Nurses (N = 2,369) working in the public healthcare sector in the UAE were asked to provide their perceptions on work engagement and its antecedents, their performance and how they perceive justice in their workplace.

Findings
Regardless of job demands, nurses’ job performance remained unaffected by demographic factors, which was a striking finding: nurses provide quality services and manage to accomplish their tasks, at any level of demand. Justice acted as a moderator of the relationship between job resources and work engagement, which was a new addition to the literature. Nurses with low overall perceptions of justice had stronger links between job resources and work engagement. Even if the level of justice was perceived as low, work engagement remained unaffected.

Originality/value
Work engagement is a critical issue, but has received little attention, with most focusing on its relationship with performance as the outcome variable. This paper has therefore enriched the literature and is significant in both country and sector.
Evaluation of risks affecting international construction joint venture (ICJV) projects in Ghana
Authors: Akunyumu Stephen , Fugar Frank , Adinyira Emmanuel 
Published: January 2023

Purpose
The failure rate of international construction joint venture (ICJV) projects has been noted to be high in developing countries due to the complexity and risky nature of construction projects in the international market. The purpose of this study is to identify and evaluate the risks facing ICJV projects in Ghana.

Design/methodology/approach
A risk register was developed through a comprehensive literature review. The identified risks were then used in a questionnaire survey involving local and foreign partners in ICJV projects in Ghana.

Findings
From a total of 74 risks identified, categorized into country-level risks, market-level risks and project-level risks, the “top ten” risks found to be the most critical risks facing ICJV projects in Ghana include unstable currency exchange rates, inflation, design changes, high-interest rate, budget overrun, cash flow problems of the client, economy fluctuation, difficulty in obtaining approval of projects from host government authorities/bureaucracy, potential financial distress of JV partner and bribery and corruption.

Originality/value
This study provides a comprehensive list of risks ICJV partners are likely to encounter on their projects in developing countries. Furthermore, this study improves on one of the major limitations of previous ICJV studies by collecting data from both partners of the ICJV, appropriate for cross-cultural examination and comparison.
How contemptuous leaders might harm their organization by putting high-performing followers in their place
Authors: De Clercq Dirk , Azeem Muhammad Umer , Haq Inam Ul 
Published: January 2023

PurposeThis study investigates how leaders react when they perceive a threat to their hierarchical position, such as by engaging in abusive supervision in ways that diminish followers’ organizational citizenship behavior. It also tests for a dual harmful role of leaders’ dispositional contempt in this process.Design/methodology/approachThree-wave survey data were collected among 231 leader–follower dyads across different industry sectors.FindingsLeaders’ beliefs that their authority is being threatened by high-performing followers can lead followers to halt their voluntary work behaviors, because leaders engage in verbal abuse. The harmful role of leaders’ dispositional contempt in this process is twofold: It enhances abusive supervision directly, and it operates as an indirect catalyst of the mediating role of abusive supervision.Practical implicationsOrganizations would be better placed to decrease the risk that disruptions of the hierarchical order, as perceived by leaders, escalate into diminished work-related voluntarism among employee bases by promoting leadership approaches that consider employees deserving of respect instead of disdain.Originality/valueThis study details how and when leaders who fear they may lose authority, evoked by the strong performance of their followers, actually discourage followers from doing anything more than their formal job duties.
Practice Briefing Analysis of the emergence and initial performance of REITs in India
Authors: Walia Siddhant , Sarkar Sankersan , Mohanty Birajit , Pal Subhabaha 
Published: January 2023

PurposeIn 2014, real estate investment trust (REIT) emerged as a new alternative investment option in India. This research aims to give an empirical authentication of the Indian REITs performance from April 2019 to July 2022 across a range of investment variables.Design/methodology/approachUsing monthly total returns in Indian Rupee, risk-adjusted Indian REIT performance and investment portfolio characteristics are examined. Indian REITs' potential in a diversified multi-asset portfolio is analysed using the mean-variance analysis, asset allocation diagram and efficient frontier.FindingsDuring April 2019–July 2022, Indian REITs provided a lower return than stocks but outperformed bonds despite coronavirus disease 2019 (COVID-19) lockdowns, which hurt the traditional working from office concept. The study also examined REIT allocation to an Indian mixed-asset portfolio and the benefits of a diversified portfolio.Practical implicationsIndian REITs provide a liquid, transparent alternative to direct property for investors seeking exposure to Indian real estate markets. Indian REITs gave real estate companies an extra funding source and investors an alternate asset. This paper explores Indian REITs' potential opportunities, given that domestic and foreign investors' demand for transparent property investment in India. The analysis found a positive early performance despite a challenging environment.Originality/valueThis paper offers the first empirical performance validation of Indian REITs as a way to obtain exposure to commercial property in India and the REITs' role in a diversified asset portfolio. The authors' study improves investors' decision-making abilities by providing empirically validated, valuable and practicable property investing insights.
Helping the helpers: the role of organizational support and peer influence on police officer receptivity to employee assistance programs
Authors: Quick Kenneth M. 
Published: January 2023

PurposeThis study aims to investigate critical differences between police officer willingness to use and recommend an employee assistance program (EAP) to a peer, including the relationship between officer perceptions of macro-level organizational support and micro-level EAP support.Design/methodology/approachA survey of 213 police officers from a large, urban police department in the Northeast United States of America is used to evaluate the relationship between officer perceptions of the EAP and the officers' willingness to use and recommend the EAP to peers. Generalized linear regression models are used to evaluate the moderating effect of perceived organizational support (POS) on perceived EAP support.FindingsThe findings of this study suggest that POS strongly predicts willingness to use the EAP, but POS has no significant relationship with willingness to recommend the EAP. There is evidence that POS moderates the relationship of EAP support on willingness to recommend the EAP to a peer. When POS decreases, the relationship of EAP support with willingness to recommend the EAP to peers becomes stronger.Research limitations/implicationsThe current study is limited by the study's focus on only one police department and the department's use of cross-sectional data, which may limit the generalizability of the results to agencies that differ in size and type.Practical implicationsThis research has practical implications for those who seek to improve officer receptivity to mental health support and improve the quality of police services provided to the public. The findings demonstrate that improving officer willingness to recommend the EAP to peers may be a more sustainable pathway to addressing officer mental health than willingness to use the EAP considering the current movement toward police reform.Originality/valueThe research unveils significant differences in police officer decision-making between using the EAP and recommending it to a peer. The research also adds empirical evidence to existing research on the role of POS in policing and the altruistic, peer-focused nature of the police subculture.