bluuebunny/crossref_metadata_2025_split huggingface dataset:
Multimodal investigation of electronic transport in PTMA and its impact on organic radical battery performance
Authors: Daniel Davis Thomas , Oevermann Steffen , Mitra Souvik , Rudolf Katharina , Heuer Andreas , Eichel Rüdiger-A. , Winter Martin , Diddens Diddo , Brunklaus Gunther , Granwehr Josef 
Published: July 2023

AbstractOrganic radical batteries (ORBs) represent a viable pathway to a more sustainable energy storage technology compared to conventional Li-ion batteries. For further materials and cell development towards competitive energy and power densities, a deeper understanding of electron transport and conductivity in organic radical polymer cathodes is required. Such electron transport is characterised by electron hopping processes, which depend on the presence of closely spaced hopping sites. Using a combination of electrochemical, electron paramagnetic resonance (EPR) spectroscopic, and theoretical molecular dynamics as well as density functional theory modelling techniques, we explored how compositional characteristics of cross-linked poly(2,2,6,6-tetramethyl-1-piperidinyloxy-4-yl methacrylate) (PTMA) polymers govern electron hopping and rationalise their impact on ORB performance. Electrochemistry and EPR spectroscopy not only show a correlation between capacity and the total number of radicals in an ORB using a PTMA cathode, but also indicates that the state-of-health degrades about twice as fast if the amount of radical is reduced by 15%. The presence of up to 3% free monomer radicals did not improve fast charging capabilities. Pulsed EPR indicated that these radicals readily dissolve into the electrolyte but a direct effect on battery degradation could not be shown. However, a qualitative impact cannot be excluded either. The work further illustrates that nitroxide units have a high affinity to the carbon black conductive additive, indicating the possibility of its participation in electron hopping. At the same time, the polymers attempt to adopt a compact conformation to increase radical–radical contact. Hence, a kinetic competition exists, which might gradually be altered towards a thermodynamically more stable configuration by repeated cycling, yet further investigations are required for its characterisation.
Containment of sulfate in leachate as gypsum (CaSO4·2H2O) mineral formation in bio-cemented sand via enzyme-induced carbonate precipitation
Authors: Kim Junghoon , Kim Daehyun , Yun Tae Sup 
Published: July 2023

AbstractEnzymatically induced carbonate precipitation (EICP) using urea hydrolysis is a well-known bio-cementation process that not only promotes the precipitation of calcium carbonate (CaCO3) but can provide excess calcium cations for further reaction depending on the substrate constituents and reaction stage. This study presents the EICP recipe to contain sulfate ions in landfill leachate sufficiently using remaining calcium cations and a series of tests were conducted to validate its ability to retain sulfates. The reaction rate for 1 M CaCl2and 1.5 M urea was identified by controlling the purified urease content and the curing time of the EICP process. The results showed that 0.3 g/L of purified urease produced 46% CaCO3and reduced sulfate ions by 77% after 3 days of curing. The shear stiffness in EICP-treated sand was enhanced 13 times by CaCO3precipitation followed by 1.12 times increment due to subsequent precipitation of gypsum (CaSO4·2H2O) crystals implying sulfate containment. A cost-efficient EICP treatment using soybean crude urease instead of lab-grade purified urease exhibited lower sulfate removal efficiency (i.e., 18%) with only nominal formation of gypsum in the EICP-treated sand. The addition of gypsum powder was effective in increasing sulfate removal by 40% when soybean crude urease was used for EICP.
A model eye for fluorescent characterization of retinal cultures and tissues
Authors: Ferraro G. , Gigante Y. , Pitea M. , Mautone L. , Ruocco G. , Di Angelantonio S. , Leonetti M. 
Published: July 2023

AbstractMany human neural or neurodegenerative diseases strongly affect the ocular and retinal environment showing peculiar alterations which can be employed as specific disease biomarkers. The noninvasive optical accessibility of the retina makes the ocular investigation a potentially competitive strategy for screening, thus the development of retinal biomarkers is rapidly growing. Nevertheless, a tool to study and image biomarkers or biological samples in a human-like eye environment is still missing. Here we report on a modular and versatile eye model designed to host biological samples, such as retinal cultures differentiated from human induced pluripotent stem cells and ex-vivo retinal tissue, but also suited to host any kind of retinal biomarkers. We characterized the imaging performance of this eye model on standard biomarkers such as Alexa Fluor 532 and Alexa Fluor 594.
Molecular determinants of epithelial mesenchymal transition in mouse placenta and trophoblast stem cell
Authors: Jena Shipra Kanti , Das Shreya , Chakraborty Shreeta , Ain Rupasri 
Published: July 2023

AbstractTrophectoderm cells of the blastocyst are the precursor of the placenta that is comprised of trophoblast, endothelial and smooth muscle cells. Since trophoectoderm cells are epithelial in nature, epithelial mesenchymal transition (EMT) of trophoblast stem (TS) cells might play pivotal role in placental morphogenesis. However, the molecular regulation of EMT during placental development and trophoblast differentiation still remained elusive. In this report, we sought to identify the molecular signature that regulates EMT during placental development and TS cell differentiation in mice. On E7.5 onwards the TS cells, located in the ectoplacental cone (EPC), rapidly divide and differentiate leading to formation of placenta proper. Using a real time PCR based array of functional EMT transcriptome with RNA from mouse implantation sites (IS) on E7.5 and E9.5, it was observed that there was an overall reduction of EMT gene expression in the IS as gestation progressed from E7.5 to E9.5 albeit the levels of EMT gene expression were substantial on both days. Further validation of array results using real time PCR and western blot analysis showed significant decrease in EMT-associated genes that included (a) transcription factors (Snai2, Zeb1, Stat3 and Foxc2), (b) extracellular matrix and cell adhesion related genes (Bmp1, Itga5, Vcan and Col3A1), (c) migration and motility- associated genes (Vim, Msn and FN1) and (d) differentiation and development related genes (Wnt5b, Jag1 and Cleaved Notch-1) on E9.5. To understand whether EMT is an ongoing process during placentation, the EMT-associated signatures genes, prevalent on E 7.5 and 9.5, were analysed on E12.5, E14.5 and E17.5 of mouse placenta. Interestingly, expression of these EMT-signature proteins were significantly higher at E12.5 though substantial expressions was observed in placenta with progression of gestation from mid- to late. To evaluate whether TS cells have the potential to undergo EMT ex vivo, TS cells were subjected to EMT induction, which was confirmed using morphological analysis and marker gene expression. Induction of EMT in TS cells showed similar gene expression profile of placental EMT. These results have broad biological implications, as inadequate mesenchymal transition leading to improper trophoblast-vasculogenic mimicry leads to placental pathophysiology and pregnancy failure.
Association of constipation with increased risk of hypertension and cardiovascular events in elderly Australian patients
Authors: Judkins Courtney P. , Wang Yutang , Jelinic Maria , Bobik Alex , Vinh Antony , Sobey Christopher G. , Drummond Grant R. 
Published: July 2023

AbstractThe association between constipation and cardiovascular risk is unclear. This population-level matched cohort study compared the association of constipation with hypertension and incident cardiovascular events in 541,172 hospitalized patients aged ≥ 60 years. For each constipation admission, one exact age-matched non-constipated admission was randomly selected from all hospitalizations within 2 weeks to form the comparison cohort. The association of constipation with hypertension and cardiovascular events (myocardial infarction, angina, stroke and transient ischemic attack) were analysed using a series of binary logistic regressions adjusting for age, sex, cardiovascular risk factors, gastrointestinal disorders and sociological factors. Patients with constipation had a higher multivariate-adjusted risk for hypertension (odds ratio [OR], 1.96; 95% confidence interval [CI] 1.94–1.99; P < 0.001). Compared to patients with neither constipation nor hypertension, there was a higher multivariate-adjusted risk for cardiovascular events in patients with constipation alone (OR, 1.58; 95% CI 1.55–1.61; P < 0.001) or hypertension alone (OR, 6.12; 95% CI 5.99–6.26; P < 0.001). In patients with both constipation and hypertension, the risk for all cardiovascular events appeared to be additive (OR, 6.53; 95% CI 6.40–6.66; P < 0.001). In conclusion, among hospital patients aged 60 years or older, constipation is linked to an increased risk of hypertension and cardiovascular events. These findings suggest that interventions to address constipation may reduce cardiovascular risk in elderly patients.
Flow cytometric detection of vancomycin-resistant Enterococcus faecium in urine using fluorescently labelled enterocin K1
Authors: Oftedal Thomas F. , Diep Dzung B. 
Published: July 2023

AbstractA urinary tract infection (UTI) occurs when bacteria enter and multiply in the urinary system. The infection is most often caused by enteric bacteria that normally live in the gut, which include Enterococcus faecium. Without antibiotic treatment, UTIs can progress to life-threatening septic shock. Early diagnosis and identification of the pathogen will reduce antibiotic use and improve patient outcomes. In this work, we develop and optimize a cost-effective and rapid (< 40 min) method for detecting E. faecium in urine. The method uses a fluorescently labelled bacteriocin enterocin K1 (FITC-EntK1) that binds specifically to E. faecium and is then detected using a conventional flow cytometer. Using this detection assay, urine containing E. faecium was identified by an increase in the fluorescent signals by 25–73-fold (median fluorescence intensity) compared to control samples containing Escherichia coli or Staphylococcus aureus. The method presented in this work is a proof of concept showing the potential of bacteriocins to act as specific probes for the detection of specific bacteria, such as pathogens, in biological samples.
SERPINA3-ANKRD11-HDAC3 pathway induced aromatase inhibitor resistance in breast cancer can be reversed by HDAC3 inhibition
Authors: Zhou Jing , Zhu Mengdi , Wang Qi , Deng Yiyuan , Liu Nianqiu , Liu Yujie , Liu Qiang 
Published: July 2023

AbstractEndocrine resistance is a major challenge for breast cancer therapy. To identify the genes pivotal for endocrine-resistance progression, we screened five datasets and found 7 commonly dysregulated genes in endocrine-resistant breast cancer cells. Here we show that downregulation of serine protease inhibitor clade A member 3 (SERPINA3) which is a direct target gene of estrogen receptor α contributes to aromatase inhibitor resistance. Ankyrin repeat domain containing 11 (ANKRD11) works as a downstream effector of SERPINA3 in mediating endocrine-resistance. It induces aromatase inhibitor insensitivity by interacting with histone deacetylase 3 (HDAC3) and upregulating its activity. Our study suggests that aromatase inhibitor therapy downregulates SERPINA3 and leads to the ensuing upregulation of ANKRD11, which in turn promotes aromatase inhibitor resistance via binding to and activating HDAC3. HDAC3 inhibition may reverse the aromatase inhibitor resistance in ER-positive breast cancer with decreased SERPINA3 and increased ANKRD11 expression.
A robust ultra-microporous cationic aluminum-based metal-organic framework with a flexible tetra-carboxylate linker
Authors: Nandi Shyamapada , Mansouri Asma , Dovgaliuk Iurii , Boullay Philippe , Patriarche Gilles , Cornu Ieuan , Florian Pierre , Mouchaham Georges , Serre Christian 
Published: July 2023

AbstractAl-based cationic metal-organic frameworks (MOFs) are uncommon. Here, we report a cationic Al-MOF, MIP-213(Al) ([Al18(μ2-OH)24(OH2)12(mdip)6]6Cl·6H2O) constructed from flexible tetra-carboxylate ligand (5,5'-Methylenediisophthalic acid; H4mdip). Its crystal structure was determined by the combination of three-dimensional electron diffraction (3DED) and high-resolution powder X-ray diffraction. The structure is built from infinite corner-sharing chains of AlO4(OH)2 and AlO2(OH)3(H2O) octahedra forming an 18-membered rings honeycomb lattice, similar to that of MIL-96(Al), a scarce Al-polycarboxylate defective MOF. Despite sharing these structural similarities, MIP-213(Al), unlike MIL-96(Al), lacks the isolated μ3-oxo-bridged Al-clusters. This leads to an ordered defective cationic framework whose charge is balanced by Cl- sandwiched between two Al-trimers at the corner of the honeycomb, showing strong interaction with terminal H2O coordinated to the Al-trimers. The overall structure is endowed by a narrow quasi-1D channel of dimension ~4.7 Å. The Cl- in the framework restrains the accessibility of the channels, while the MOF selectively adsorbs CO2 over N2 and possesses high hydrolytic stability.
Human resident liver myeloid cells protect against metabolic stress in obesity
Authors: Barreby Emelie , Strunz Benedikt , Nock Sebastian , Naudet Léa , Shen Joanne X. , Johansson Helene , Sönnerborg Isabella , Ma Junjie , Urgard Egon , Pallett Laura J. , Hu Yizhou , Fardellas Achilleas , Azzimato Valerio , Vankova Ana , Levi Laura , Morgantini Cecilia , Maini Mala K. , Stål Per , Rosshart Stephan P. , Coquet Jonathan M. , Nowak Greg , Näslund Erik , Lauschke Volker M. , Ellis Ewa , Björkström Niklas K. , Chen Ping , Aouadi Myriam 
Published: July 2023

AbstractAlthough multiple populations of macrophages have been described in the human liver, their function and turnover in patients with obesity at high risk of developing non-alcoholic fatty liver disease (NAFLD) and cirrhosis are currently unknown. Herein, we identify a specific human population of resident liver myeloid cells that protects against the metabolic impairment associated with obesity. By studying the turnover of liver myeloid cells in individuals undergoing liver transplantation, we find that liver myeloid cell turnover differs between humans and mice. Using single-cell techniques and flow cytometry, we determine that the proportion of the protective resident liver myeloid cells, denoted liver myeloid cells 2 (LM2), decreases during obesity. Functional validation approaches using human 2D and 3D cultures reveal that the presence of LM2 ameliorates the oxidative stress associated with obese conditions. Our study indicates that resident myeloid cells could be a therapeutic target to decrease the oxidative stress associated with NAFLD.
A NiS2/C composite as an innovative anode material for sodium-ion batteries: ex situ XANES and EXAFS studies to investigate the sodium storage mechanism
Authors: Shaikh Shoyebmohamad F. , Aftab Sikandar , Pandit Bidhan , Al-Enizi Abdullah M. , Ubaidullah Mohd , Ekar Satish , Hussain Sajjad , Khollam Yogesh B. , More Pravin S. , Mane Rajaram S. 
Published: July 2023

Ex situ XANES and EXAFS studies of NiS2/C electrode to investigate sodium storage mechanism.
Distributed parameter identification algorithm for large‐scale interconnected systems
Authors: Hamdi Mounira , Idomhgar Lhassane , Kamoun Samira , Chaoui Mondher , Kachouri Abdenaceur 
Published: July 2023

AbstractThis paper deals with parameter estimation problem of large‐scale systems. A recursive distributed parameter estimation algorithm, based on the minimization of the prediction estimation error method, is developed. Specifically, the class of large‐scale systems that are composed of several interconnected sub‐systems is considered. Each interconnected sub‐system is modelled by a linear discrete‐time state space mathematical model with unknown parameters. The convergence analysis is then achieved using the Lyapunov approach. The theoretical analysis and simulation results prove the effectiveness of the proposed algorithm.
Particle motion in ultra-strong electromagnetic fields of neutron stars: The influence of radiation reaction
Authors: Tomczak Ivan , Pétri Jérôme 
Published: July 2023

Context. Neutron stars are known to be efficient accelerators that produce particles with ultra-relativistic energies. As a by-product, they also emit copious amounts of photons from radio wavelengths up to gamma rays.
Aims. As a follow-up to our previous work on particle acceleration simulation near neutron stars, in this paper, we discuss the impact of radiation reaction on test particles injected into their magnetosphere. We therefore neglect the interaction between particles through the electromagnetic field as well as gravitation.
Methods. We integrate numerically the reduced Landau-Lifshitz equation for electrons and protons in the vacuum field of a rotating magnetic dipole based on analytical solutions in a constant electromagnetic field. These expressions are simple in a frame where the electric and magnetic field are parallel. Lorentz transforms are used to switch back and forth between this frame and the observer frame.
Results. We found that, though due solely to the Lorentz force, electrons reach Lorentz factors up to γ = 1014 and protons reach them up to γ = 1010.7. When radiation reaction is enabled, electrons reach energies up to γ = 1010.5 and protons reach energies up to γ = 108.3. The second set of values are more realistic since the radiation reaction feedback is predominant within the magnetosphere. Moreover, as expected, symmetrical behaviours between the north and south hemispheres are highlighted, either with respect to the location around the neutron star or with respect to particles of opposite charge to mass ratio (q/m). Consequently, it is useless to simulate the full set of geometrical parameters in an effort to obtain an overview of all possibilities.
Conclusions. The study of the influence of the magnetic dipolar moment inclination shows similar behaviours regardless of whether radiation reaction is enabled. Protons (respectively electrons) impact the surface of the neutron star less as the inclination angle increases (decreases for electrons), while if the rotation and magnetic axes are aligned, all the protons impact the neutron star, and all the electrons impact the surface if the rotation and magnetic axes are anti-aligned. Similarly, we still find that particles are ejected away from the neutron star, in some preferred directions and Lorentz factors.
Observations of neutral carbon in 29 high-z lensed dusty star-forming galaxies and the comparison of gas mass tracers
Authors: Gururajan G. , B’ethermin M. , Sulzenauer N. , Theulé P. , Spilker J. S. , Aravena M. , Chapman S. C. , Gonzalez A. , Greve T. R. , Narayanan D. , Reuter C. , Vieira J. D. , Weiss A. 
Published: July 2023

Context. The nature and evolution of high-redshift dusty star-forming galaxies (high-z DSFGs) remain open questions. Their massive gas reservoirs play an important role in driving the intense star formation rates hosted in these galaxies.
Aims. Our aim is to estimate the molecular gas content of high-z DSFGs by using various gas mass tracers, such as the [CI], CO, [CII] emission lines and the dust content. These tracers need to be well calibrated as they are all limited by uncertainties on factors such as αCO, XCI, α[CII], and δGDR, thereby affecting the accurate determination of the gas mass. The main goal of our work is to check the consistency between the gas mass tracers and to cross-calibrate the uncertain factors.
Methods. We observed the two [CI] line transitions for 29 South Pole telescope Submillimeter Galaxies (SPT-SMGs) with the Atacama Large Millimeter/submillimeter Array–Atacama Compact Array (ALMA-ACA). Additionally, we also present new Atacama Pathfinder EXperiment (APEX) observations of [CII] line for nine of these galaxies. We combined our observations with the rich ancillary data of low- and mid-J CO lines, ancillary [CII] line, and dust mass estimations for these galaxies.
Results. We find a nearly linear relation between the infrared luminosity and [CI] luminosity if we fit the starbursts and main-sequence galaxies separately. We measure a median [CI]-derived excitation temperature of 34.5 ± 2.1 K. We probed the properties of the interstellar medium (ISM), such as density and radiation field intensity, using [CI] to mid- or high-J CO lines and the [CI]-to-infrared luminosity ratio, and find similar values to the SMG populations in the literature. Finally, the gas masses estimated from [CI], CO, dust, and [CII] do not exhibit any significant trend with the infrared luminosity or the dust temperature. We provide the various cross-calibrations between these tracers.
Conclusions. Our study confirms that [CI] is a suitable tracer of the molecular gas content, and shows an overall agreement between all the classical gas tracers used at high redshift. However, their absolute calibration, and thus the gas depletion timescale measurements, remain uncertain.
Adherence to Positive Airway Pressure Therapy in a Cohort of Colombian Patients with Obstructive Sleep Apnea Syndrome
Authors: Ortiz Judith L. , Rosselli Diego 
Published: July 2023

Abstract
Objective To describe the adherence to the use of positive air pressure (PAP) devices in a cohort of patients with sleep apnea syndrome in Colombia.
Material and Methods Descriptive cross-sectional study of adult patients treated between January 2018 and December 2019 in the sleep clinic of a private insurer in Colombia.
Results The analysis included 12,538 patients (51.3% women) with a mean age of 61.3 years; 10,220 patients (81.5%) use CPAP and 1,550 (12.4%) BIPAP. Only 37% are adherent (> 70% of use for 4 hours or more), adherence rates were highest in the >65 years age groups. 2,305 patients (18.5%) were hospitalized, on average 3.2 times; 515 of these (21.3%) had one or more cardiovascular comorbidities.
Conclusion Adherence rates in this sample are lower than those reported elsewhere. They are similar in males and females and tend to improve with age.