bluuebunny/crossref_metadata_2025_split huggingface dataset:
The Application of 3base™ Technology to Diagnose Eight of the Most Clinically Important Gastrointestinal Protozoan Infections
Authors: Aghazadeh Mahdis , Jones Meghan , Perera Suneth , Nair Jiny , Tan Litty , Clark Brett , Curtis Angela , Jones Jackson , Ellem Justin , Olma Tom , Stark Damien , Melki John , Coulston Neralie , Baker Rohan , Millar Douglas 
Published: August 2023

Globally, over 3.5 billion people are infected with intestinal parasites each year, resulting in over 200,000 deaths. Three of the most common protozoan pathogens that affect the gastrointestinal tract of humans are Cryptosporidium spp., Giardia intestinalis, and Entamoeba histolytica. Other protozoan agents that have been implicated in gastroenteritis in humans include Cyclospora cayetanensis, Dientamoeba fragilis, Blastocystis hominis, and the microsporidia Enterocytozoon bieneusi and Encephalitozoon intestinalis. Genetic Signatures previously developed a 3base™ multiplexed Real-Time PCR (mRT-PCR) enteric protozoan kit (EP001) for the detection of Giardia intestinalis/lamblia/duodenalis, Cryptosporidium spp., E. histolytica, D. fragilis, and B. hominis. We now describe improvements to this kit to produce a more comprehensive assay, including C. cayetanensis, E. bieneusi, and E. intestinalis, termed EP005. The clinical performance of EP005 was assessed using a set of 380 clinical samples against a commercially available PCR test and other in-house nucleic acid amplification tests where commercial tests were not available. All methods provided at least 90% agreement. EP005 had no cross-reactivity against 82 organisms commonly found in the gut. The EP005 method streamlines the detection of gastrointestinal parasites and addresses the many challenges of traditional microscopic detection, resulting in cost savings and significant improvements in patient care.
Evaluation of Indicators of the Immune System in Patients Suffering from Chronic Viral Hepatitis B
Authors: Мухамедов Б.И. , Мухамедов И.М. , Ризаев Ж.А. , Колдарова Э.В. , Алиев А.Ш. 
Published: August 2023

Проведен анализ показателей неспецифической и специфической резистентности у пациентов с хроническим вирусным гепатитом В. Представлены относительные показатели СD3 и CD19 регулярных субпопуляций Т-звена иммунитета (CD4 и CD8), N-лимфоцитов, иммунорегуляторного индекса (CD4/CD8). После лечения бификолом на фоне традиционной терапии у пациентов с хроническим вирусным гепатитом В было отмечено, что все показатели клеточного и гуморального иммунитета, а также параметры неспецифической резистентности были достоверно выше, чем у пациентов, не получавших пробиотик.
The analysis of indicators of nonspecific and specific resistance in patients with chronic viral hepatitis B was carried out. Relative indicators of CD3 and CD19 of regular subpopulations of the T-link of immunity (CD4 and CD8), N-lymphocytes, immunoregulatory index (CD4/ CD8) are presented. After treatment with bificol against the background of traditional therapy in patients with chronic viral hepatitis B, it was noted that all indicators of cellular and humoral immunity, as well as parameters of nonspecific resistance, were significantly higher than in patients who did not receive the probiotic.
Studi pengaruh penuaan minyak trafo terhadap karakteristik dielektrik
Authors: Saodah Siti , Eliana Bella , Jakariya Jakariya 
Published: August 2023

Trafo adalah peralatan penting dalam transmisi tenaga listrik. Berbagai jenis bahan insulasi yang digunakan dalam transformator diantaranya minyak mineral atau minyak transformator digunakan untuk insulasi. Dalam upaya mengurangi persentase kegagalan transformator, perlu untuk menjaga kemurnian minyak. Pada penelitian ini menyelidiki karakteristik dielektrik minyak transformator yang telah menglami penuaan dan minyak transformator murni. Sampel minyak trafo yang mengalami penuaan dibuat dengan menggunakan minyak transformator murni yang dipanaskan pada suhu tertentu dan dalam siklus yang telah ditentukan. Electric field intensity minyak trafo diidentifikasi untuk melihat dielectric strength dari minyak trafo yang mengalami penuaan. Dalam mengidentifikasi electric field intensity, analisis FEM dilakukan. Hasil penelitian menunjukkan bahwa dielectric strength minyak trafo baik pada tipe Shell Diala maupun Triapar mengalami degradasi sekitar 20% dan 40% pada suhu penuaan 130 °C dan 150 °C secara berurutan pada penerapan tegangan AC. Dengan begitu, pemberian tekanan thermal memengaruhi sifat dielektrik dari minyak trafo.
Efek Antibakteri Ekstrak Kulit Kelengkeng (Dimocarpus Longan L.) terhadap Porphyromonas gingivalis
Authors: Tobaq Fritzia R. , Mandalas Henry Y. , Sugiaman Vinna K. 
Published: August 2023

Abstract: Porphyromonas gingivalis is one of the microorganisms that cause periodontitis. Chlorhexidine is proven to have an antibacterial effect but it can cause side effects inter alia discolored teeth, pain, and xerostomia. Longan peel is a natural product that can act as an antibacterial because of its active compounds such as phenolics, tannins, flavonoids, and triterpenoids. This study aimed to determine the antibacterial effect of longan peel extract on Porphyromonas gingivalis ATCC 33277 by measuring the average diameter of the inhibition zone. This was a laboratory experimental study using experimental groups at concentrations of 3.125%, 6.25%, 12.5%, 25%, 50%, 75%, and 100%. The results showed that the experimental group at a concentration of 100% extract produced an inhibition zone of 10.08 mm but not greater than 0.2% chlorhexidine which had 11.96 mm. While the smallest inhibition zone was conducted by longan peel with 25% extract which was 4.05 mm. In conclusion, there is an antibacterial effect of the ethanol extract of longan peel (Dimocarpus longan L) against Porphyromonas gingivalis.
Keywords: chronic periodontitis; longan peel extract (Dimocarpus longan L.); well diffusion method; antibacterial effectiveness

Abstrak: Porphyromonas gingivalis merupakan salah satu mikroorganisme penyebab periodontitis. Penggunaan chlorhexidine terbukti dapat memberikan efek antibakteri, tetapi dapat menyebabkan efek samping berupa perubahan warna gigi, nyeri, dan xerostomia. Kulit kelengkeng sebagai bahan alam dengan kandungan senyawa aktifnya seperti fenolik, tanin, flavonoid, dan triterpenoid dapat berperan sebagai antibakteri. Penelitian ini bertujuan untuk mengetahui efek antibakteri ekstrak kulit kelengkeng terhadap bakteri Porphyromonas gingivalis ATCC 33277 dengan cara mengukur rerata diameter dari zona hambat. Penelitian ini menggunakan metode eksperimental laboratorium dengan kelompok perlakuan pada konsentrasi ekstrak 3,125%, 6,25%, 12,5%, 25%, 50%, 75%, dan 100%. Hasil penelitian memperlihatkan bahwa pada kelompok perlakuan dengan konsentrasi ekstrak 100% menghasilkan zona hambat 10,08 mm tetapi tidak lebih besar dari chlorhexidine 0,2% yang memiliki zona hambat 11,96 mm. Zona hambat terkecil dihasilkan oleh kelompok ekstrak 25% yaitu sebesar 4,05 mm. Simpulan penelitian ini ialah terdapat efek antibakteri ekstrak etanol kulit kelengkeng (Dimocarpus longan L) terhadap Porphyromonas gingivalis.
Kata kunci: periodontitis kronis; ekstrak kulit kelengkeng (Dimocarpus longan L.); metode difusi sumuran; efektivitas antibakteri
DIVA MOLECULAR DETECTION OF SUSPECTED CASE OF NEWCASTLE AND ENCEPHALOMYLITIS DISEASE IN LAYERS
Authors: Amjed. H. Ulaiwi  
Published: August 2023

The aim of the study investigates the molecular diagnosis between (the Avian encephalomyelitis virus and Newcastle disease virus and differentiating the infected from vaccinated animals (DIVA) strategies. The 1st detection by RT-PCR for(PMV-1) as primary detection then, the samples were prepared as in FTA card Whatman® and sent to (AniCon Labs) Germany to detect by one step RT-PCR (DIVA technique) between NDV and AEV genotype. The result of the molecular investigation as primary detection revealed three samples were positive with Ct values (34.0, 26.6, and 35.8) respectively. Then, in the detection of AEV the result showed all samples were negative. finally, by using (DIVA) with two primers to detect general (PMV1-9) by (M-gene) all samples were positive at Ct values (20.8, 20.1, and 25.2) respectively, and then path type by (F gene) to differentiate infected from vaccinated samples also all samples were positive with Ct values (23.8, 23.3 and 23.1) respectively. the study concluded that the samples can differentiate between AEV and NDV by using DIVA strategies to find pathotypes and differentiating between infected and vaccinal isolate based on certain amino acids in the primers.
STUDYING THE FORMATION AND STABILIZATION OF CUR-AL+3 COMPLEXES BY USING CITRIC ACID AS CATALYST
Authors: Zina B. Muhammed  , Tariq N. Musa  
Published: August 2023

Cooking ware that is made of aluminum is used in different nations, particularly developing nations, and are a free source of aluminum. Due to its possible harmful effects on human health, aluminum is acknowledged as a public health problem. The goal of the current investigation is to evaluate curcumin's (CUR) capacity to decrease aluminium’s toxicity by forming stable complexes. Rhizomes that are sold in the local marketplaces have an 8.5% crude pigment content of CUR. At various pH levels, CUR's maximum absorbance was identified. Citric acid was used as a catalyst to create the CUR-Al+3 combination at various pH levels, which correspond to the rates at which Al+3 was eliminated from the solution. At pH 2.5, 3.0, 3.5, and 4.0, the highly complexation was seen. At such pH levels, the Al+3 elimination was 79.22, 78.28, 79.26, and 79.41%, respectively. The CUR-Al+3 complexes’ stability represent, respectively, 96.94, 96.29, 95.55, and 95.52% of the initial concentration at 25o, 50o, 75o, and 100o C for 60 min at pH value of 2.50. Using Fourier-transform infrared spectroscopy (FT-IR), it has been possible to identify CUR and its complex with Al+3. At varied pH levels, 1.5, 2.5, 3.5, 4.5, 5.5, and 6.5 at boiling point, the leached aluminium from 3 regions of the aluminium cooking ware (Syrian, Iranian and local) was specified. Comparatively to Syrian and local cooking wares, Iranian cooking ware generally leached more aluminum. In comparison to other pH values, the percentages of the elimination of the aluminum that is leached by utilizing the most leaching cookware (Iranian) have been 79.23 and 79.26% at pH values of 2.50 and 3.50, respectively.
BIODIVERSITY AND ABUNDANCE OF PHYTOPLANKTON IN RICE-FISH FARMING SYSTEM
Authors: Mustika Palupi  , Kasprijo  , Rudi Wijaya  , Rizky Ade Chanda  , Rais Fikri Azhari  , Ren Fitriadi  
Published: August 2023

Rice-fish farming system that is purposed to maximize land use by adding fish farming to its systems. In the Rice-fish farming system, the fish farm serves as a solution to overcome pests so it can provide benefits for the farmers. Fish farm maintenance is considered to be a solution to reduce farmers expenditures in the use of fertilizers. The reason is in the fish-maintaining process, fish will naturally excrete dirt which has a function as organic fertilizer which will increase the fertility of the waters. The purpose of this study was to determine the composition of phytoplankton as well as the physical and chemical quality of the water in several Rice-fish farming system stations in Panembangan Village so that the condition of the ecosystem can be detected whether it is in good or bad condition. The Results showed that the diversity index value ranged from 0.61-1.99. The uniformity index value was obtained in the range of 0.15 – 0.50 and the dominance index value was obtained in the range of 0.20 – 0.72. While, the four stations still have good water quality, the conditions in the Rice-fish farming system area of Panembangan Village are still suitable for the growth of organisms.
ANALISIS SALURAN DAN EFISIENSI PEMASARAN PESTISIDA DI KABUPATEN KONAWE (Studi Kasus Pada Toko Sahabat Tani)
Authors: Saranani Milawati 
Published: August 2023

Penelitian ini bertujuan untuk menganalisa saluran pemasaran pestisida Pada Toko Sahabat Tani di Kecamatan Unaaha Kabupaten Konawe dan untuk menganalisa tingkat efisiensi pemasaran pestisida Pada Toko Sahabat Tani di Kecamatan Unaaha Kabupaten Konawe. Penentuan sampel yang dilakukan yaitu menentukan informan kunci (key person) dan teknik Snow Ball Sampling. Responden dalam penelitian adalah distributor UD. Sahabat Tani, 4 kios tani di wilayah pemasaran pestisida, serta 12 orang petani sebagai konsumen. Berdasarkan hasil penelitian saluran pemasaran pestisida yang terjadi dalam wilayah penelitian ini terdapat 2 jenis saluran pemasaran yaitu Saluran I = dari Toko Sahabat Tani sebagai distributor ke pedagang pengecer selanjutnya ke petani, dan saluran II dari Toko Sahabat Tani selanjutnya ke konsumen/petani. Nilai efisiensi pemasaran pestisida memperlihatkan bahwa distributor diperoleh nilai efisiensi sebesar 84% sedangkan pedagang pengecer memperoleh nilai efisien sebesar 82%. Hal ini menunjukkan bahwa distributor dan pedagang pengecer sudah efisien dalam melakukan pemasaran Pestisida.
Kata Kunci : Saluran, Efisiensi, Pemasaran, Pestisida.

ABSTRACT

This study aims to analyze the pesticide marketing channel at Sahabat Tani Stores in Unaaha District, Konawe Regency and to analyze the efficiency level of pesticide marketing at Sahabat Tani Stores in Unaaha District, Konawe Regency. The sample determination was carried out by determining key informants and the Snow Ball Sampling technique. Respondents in the study were UD distributors. Sahabat Tani, 4 farmer stalls in the pesticide marketing area, and 12 farmers as consumers. Based on the research results of pesticide marketing channels that occur in this research area, there are 2 types of marketing channels, namely Channel I = from Sahabat Tani Shops as distributors to retailers then to farmers, and channel II from Sahabat Tani Shops to consumers/farmers. The efficiency value of pesticide marketing shows that the distributor obtained an efficiency value of 84% while the retailer obtained an efficiency value of 82%. This shows that distributors and retailers are efficient in marketing pesticides.
Keywords: Channels, Efficiency, Marketing, Pesticides.
PREFERENSI PETANI PADI DALAM PEMANFAATAN E-COMMERCE SEBAGAI SARANA PEMASARAN
Authors: Haryanto Yoyon , Harniati Harniati , Cubeba Farah Az-zahra , Wibowo Haris Tri 
Published: August 2023

Perubahan perilaku konsumsi masyarakat Indonesia sebagai dampak dari adanya pandemi seperti dalam hal mencari informasi, menggunakan, maupun membeli barang maupun jasa. Penggunaan teknologi informasi dalam perdagangan dan jasa salahsatunya dengan memanfaatkan e-commerce untuk mengakses peluang pasar yang lebih. Sistem penjualan konvensional cenderung minim akan informasi khususnya dalam hal persaingan harga pasaran.Para petani padi di Kabupaten Cianjur masih menggunakan sistem konvensional dalam menjual hasil panennya. Pengkajian ini dilakukan untuk mendeskripsikan tingkat preferensi petani padi (Oryza sativa L.) dalam penggunaan platform e-commerce sebagai sarana pemasaran, menganalisis faktor-faktor yang mempengaruhi preferensi petani padi (Oryza sativa L.) dalam pemilihan e-commerce, menentukan strategi dalam meningkatkan preferensi petani padi (Oryza sativa L.) dalam pemanfaatan e-commerce sebagai sarana pemasaran.Berdasarkan hasil pengkajian dari 63orang sampel responden, menjelaskan bahwa tingkat preferensi petani padi terhadap penggunaan e-commerce sebagai sarana pemasaran berada kategori rendah (7%).Faktor yang berpengaruh terhadap preferensi petani padi (Oryza sativaL.) dalam pemanfaatan e-commercesebagai sarana pemasaran secara signifikan yaitu kesesuaian dengan kebutuhan (Sig.0,05).
COGNITIVE BEHAVIOR THERAPY UNTUK MENGURANGI KECEMASAN PEMBELAJARAN JARAK JAUH
Authors: Ayu Nanda Oktavia Dira , Moesarofah  
Published: August 2023

Pembelajaran jarak jauh memiliki perbedaan dengan pembelajaran konvensional. Pembelajaran jarak jauh menuntut siswa menerapkan blended learning secara mandiri. Tujuan penelitian adalah melihat adanya pengaruh Cognitive Behavior Therapy dalam konseling kelompok dengan teknik menghentikan pikiran negatif untuk menurunkan kecemasan pembelajaran jarak jauh. Desain penelitian adalah pra-eksperimental one group pretest-postest design. Sampel penelitian adalah siswa kelas XI SMAN Krian Sidoarjo, sebanyak 11 orang. Pengumpulan data mengadaptasi skala pengukuran kecemasan dari Beck Anxiety Inventory (BAI), dan teknik analisis data menggunakan uji wilcoxon. Hasil statistik menunjukkan bahwa nilai signifikansi sebesar 0,003 (Sig. < 0.05), sehingga hipotesis alternatif (Ha) diterima, dan disimpulkan terjadi penurunan signifikan tingkat kecemasan setelah diberikan Cognitive Behavior Therapy pada siswa. Penelitian ini dapat menjadi informasi bagi konselor dalam memberikan layanan pada siswa yang mengalami kecemasan pembelajaran jarak jauh serta menjadi sumber pengetahuan dalam penelitian khususnya mengatasi kecemasan akademik.

Kata Kunci: Cognitive Behavior Therapy, konseling kelompok, kecemasan pembelajaran jarak jauh.
O papanicolau como exame de rastreio do câncer de colo de útero e suas implicações na pandemia
Authors: Saturnino Aline de Fátima Rodrigues , Ferreira Alexandre Júnio Silva , Bráz Alzira Aparecida de Souza , Martins Deivson de Oliveira , Barbosa Letícia Nascimento , Fonseca Isabelle Felix , De Paula Mateus Gonçalves , Resende Fernanda Amaral 
Published: August 2023

O câncer de colo uterino é, em sua grande maioria, causado pela infecção do Papiloma vírus humano e é uma doença de progressão lenta que pode não manifestar sintomas em suas fases iniciais. Tal infecção é frequente e pode provocar alterações celulares que evoluem para o câncer. Essas alterações são facilmente identificadas por meio do exame preventivo que é usado para rastreamento da doença . Dessa forma, tem-se a importância da realização periódica deste exame. Portanto, é importante relatar o impacto da pandemia no ano de 2020 sobre a realização de exames citopatológicos de colo de útero. Assim, este estudo é classificado como ecológico do tipo retrospectivo, com abordagem de série temporal e censitária através da análise de dados quantitativos da cidade de Sinop, sobre o número de exames citopatológicos de colo de útero realizados no município, coletados no SISCAN do sistema DATASUS. Foi observado por meio deste estudo que no ano de 2020, devido ao início da Pandemia de COVID-19, houve um impacto negativo no número de consultas eletivas e dificuldade de acesso às unidades de saúde. dessa forma, pode-se atestar, de acordo com dados do SISCAN no ano de 2020, que um dos impactos da pandemia foi a não realização do exame citopatológico pela população. Podendo concluir que de 2014 a 2019 houve um aumento significativo da realização de exames Papanicolau, na cidade de Sinop-MT. Porém, em 2020 houve uma redução da realização desse exame, e aliado a isso teve o início da pandemia por covid-19, impactando diretamente no rastreio de câncer de colo de útero. Consequentemente, diminui o número de diagnósticos desse câncer em estágios iniciais.
Kualitas Air Bersih pada Sumur Bor di Desa Sumber Rejo Kabupaten Banyuasin
Authors: Ulfa Maria Ulfah , Wisnu Sugiri  
Published: August 2023

According to WHO calculations, in developed countries each person needs between 60-120 liters of water per day. One of the most important uses of water is the need for drinking. Based on field observations, the area in Sumber Rejo Village, Banyuasin Regency is a water area that allows the water quality of drilled wells in the area to not meet the standard, therefore this study aims to determine the iron content (Fe) in drilled wells. This research is descriptive quantitative. The population in this study were 12 drilled wells in the village of Sumber Rejo, Banyuasin Regency and the sampling technique was purposive sampling. The data analysis used in this study was univariate. This research was carried out 7july-7august 2021. The results showed that the content of iron (Fe) in the drilled well contained 1 of 12 samples that did not meet the requirements, the taste of the water 100% met the requirements for clean water, the turbidity of the water 100% met the requirements for clean water, and the color in the drilled well there is 1 of 12 samples that do not meet the requirements based on the Regulation of the Minister of Health of the Republic of Indonesia NO. 416/MENKES/PER/Lx/1990. It was concluded from 12 samples of drilled well water, it can be concluded that 90% of drilled well water has met the requirements of PERMENKES RI No. 416 of 1990. It is recommended that in the future the community has or uses water filters/filters before using bore well water to make it cleaner.
Gambaran Karakteristik, Pengetahuan dan Dukungan Keluarga pada Ibu Hamil dengan Anemia
Authors: Alifia Putri Crismonica 
Published: August 2023

Abstract
Anemia in pregnancy is condition that the level of hemoglobin is under 11 g/dL in first and third trimester and under 10,5 g/dL in second trimester. Anemia in pregnancy can cause abortion, low birth weight and death. This study aims to find out the descriptive of characteristic, knowledge and family support in pregnant women. This research is simple descriptive research using purposive sampling. The 94 samples were recruited with purposive sampling. The measuring instrument used questionnare that have been tested for validity and reability and distributed online used google form. The analysis used the descriptive analysis to know the frequency distribution of characteristic, knowledge and family support. Majority of age were 20-35 years as many as 87,2%, majority of maternal age were third trimester as many as 59,6%, majority of pregnancy were primigravida as many as 51,1%, majority of educational were university as many as 53,2%, majority of working status were not working as many as 63,8%, majority of economic status were low as many as 43,6%, majority of knowledge were moderate as many as 53,2% and majority of family support were good as many as 51%. Pregnant women with anemia in this research had characteristics 20-35 years, third trimester pregnancy, primigravida pregnancy, university education, not working status, low economic status, had moderate knowledge and good family support. It’s suggested for nurse to give health education about anemia more frequent to increase women pregnancy knowledge about anemia and the family can give good support to pregnant women.
Keywords: Anemia, Knowledge, Family Support, Preganncy
Identifikasi Keterpaparan Promosi Susu Formula Terhadap Pemberian Asi Eksklusif
Authors: Putri Yuliantie  , Hairiana Kusvitasari  , Frani Mariana  
Published: August 2023

Pemberian ASI Eksklusif merupakan salah satu program pembangunan secara global, Tercapainya target pemerintah Indonesia tentang ASI eksklusif berarti ikut membantu dunia dalam menyukseskan tujuan dari SDGs. Tujuan yang paling erat kaitannya dengan ASI eksklusif adalah tujuan SDGs nomor dua yaitu tentang kelaparan. ketidakberhasilan ASI Eksklusif dipengaruhi oleh berbagai faktor salah satunya dengan kosumsi susu formula. Maraknya iklan susu formula menyebabkan banyak ibu beranggapan bahwa susu formula bukan sekedar makanan, tetapi juga sebagai obat bagi anak. Hal ini diyakini oleh para ibu yang mempunyai pengetahuan kurang tentang ASI yang beranggapan bahwa susu formula lebih baik dari pada ASI karena bersifat praktis dan kandungan zat gizi penting yang tertera pada iklan susu formula. Tujuan dalam penelitian untuk mengidentifikasi keterpaparan promosi susu formula terhadap pemberian ASI Ekkslusif. Desain penelitian ini adalah survei analitik dengan pendekatan Cross sectional. Populasi pada penelitian ini adalah 159 ibu yang bayinya berumur 6-12 bulan di wilayah kerja Puskesmas Sleman. Hasil Uji statistic analisis bivariat tingkat pendidikan p-value 0.808, status pekerjaan p-value 0,001 dan keterpaparan promosi susu formula p-value 0.813.