bluuebunny/crossref_metadata_2025_split huggingface dataset:
In situ Forming Cryomicroneedles for Intradermal Cell Delivery
Authors: Xu Chenjie , Zheng Mengjia , Hu Tianli , Yang Huaxin , Zhang Yuyue , Zhang Qizheng , Yang Yating , Liu Wei , Yong Ken-Tye 
Published: September 2023

Abstract
Cryomicroneedles (cryoMNs) offer a convenient and minimally invasive way for the precise intradermal delivery of therapeutic cells in treating local and systematic diseases. They are manufactured by shaping and freezing the cell-containing cryogenic media in the microneedle template, allowing the package of cells in advance and direct usage in clinics. However, the current cryoMNs require cold-chain transportation and storage, and don’t permit the loading of the autologous cells in situ. This article introduces an in situ forming cryoMNs or the second generation of cryoMNs (S-cryoMNs) that address these limitations. Specifically, S-cryoMNs are made through dipping a porous MN scaffold in the cell suspension before cryopreservation. The porous scaffold can be transported at room temperature and researchers can load any cells with the optimized cryogenic medium. As a proof-of-concept, we examined the loading and intradermal delivery of three cell types in clinically relevant in vitro and in vivo models including mesenchymal stem cells for wound healing, melanocytes for vitiligo treatment, and antigen-pulsed dendritic cells for cancer vaccination.
Physicians’ Perspectives on AI in Clinical Decision Support Systems: Interview Study of the CURATE.AI Personalized Dose Optimization Platform
Authors: Vijayakumar Smrithi , Lee V Vien , Leong Qiao Ying , Hong Soo Jung , Blasiak Agata , Ho Dean 
Published: September 2023

Background
Physicians play a key role in integrating new clinical technology into care practices through user feedback and growth propositions to developers of the technology. As physicians are stakeholders involved through the technology iteration process, understanding their roles as users can provide nuanced insights into the workings of these technologies that are being explored. Therefore, understanding physicians’ perceptions can be critical toward clinical validation, implementation, and downstream adoption. Given the increasing prevalence of clinical decision support systems (CDSSs), there remains a need to gain an in-depth understanding of physicians’ perceptions and expectations toward their downstream implementation. This paper explores physicians’ perceptions of integrating CURATE.AI, a novel artificial intelligence (AI)–based and clinical stage personalized dosing CDSSs, into clinical practice.

Objective
This study aims to understand physicians’ perspectives of integrating CURATE.AI for clinical work and to gather insights on considerations of the implementation of AI-based CDSS tools.

Methods
A total of 12 participants completed semistructured interviews examining their knowledge, experience, attitudes, risks, and future course of the personalized combination therapy dosing platform, CURATE.AI. Interviews were audio recorded, transcribed verbatim, and coded manually. The data were thematically analyzed.

Results
Overall, 3 broad themes and 9 subthemes were identified through thematic analysis. The themes covered considerations that physicians perceived as significant across various stages of new technology development, including trial, clinical implementation, and mass adoption.

Conclusions
The study laid out the various ways physicians interpreted an AI-based personalized dosing CDSS, CURATE.AI, for their clinical practice. The research pointed out that physicians’ expectations during the different stages of technology exploration can be nuanced and layered with expectations of implementation that are relevant for technology developers and researchers.
PREDICTION OF HIGH-RISK NSSNPS ASSOCIATED WITH WISP3 GENE EXPRESSION: AN IN SILICO STUDY
Authors: M. S. SAUNDARYA , DINESH SUSHA , SHARMA SAMEER 
Published: September 2023

Objective: The primary aim of this investigation is to comprehensively examine the detrimental effects of non-synonymous single nucleotide polymorphisms (nsSNPs) on the WISP3 gene. This objective will be accomplished through intricate evaluations encompassing protein stability prediction, amino acid conservation analysis, investigation of protein-protein interactions (PPI), scrutiny of post-translational modifications (PTM), and the utilization of bioinformatics tools to forecast the potential association between nsSNPs and various diseases. By implementing these sophisticated methodologies, we aim to unveil the intricate mechanisms by which harmful nsSNPs influence the functionality and pathological implications of the WISP3 gene.
Methods: Retrieved rsIDs of SNPs from the dbSNP database and filtered using 5 in silico programs. Selected nsSNPs were subjected to further analysis i.e., protein stability and conservation analysis, solvent accessibility analysis, PPI and PTM analysis, prediction and evaluation of both native and mutant protein, and identification of cancer association and gene expression analysis.
Results: The study found that seven (C122Y, C145Y, C52Y, C78R, C75G, N233K, and R245I) of the nsSNPs are potentially vulnerable due to their higher conservancy and ability to reduce protein stability. Two (D271N and Q56H) of the nsSNPs from the initial screening were found to be associated with colon adenocarcinoma.
Conclusion: The study's findings could help researchers design experiments to validate the predictions and develop potential treatments for diseases associated with the WISP3 gene.
IMPLICATION OF CENTRAL COMPOSITE DESIGN IN THE DEVELOPMENT OF SIMVASTATIN-LOADED NANOSPONGES
Authors: NOOTHI SADHANA , MALOTHU NARENDER , ARETI ANKARA , DESU PRASANNA KUMAR , KUMAR SARVAN 
Published: September 2023

Objective: The present study’s objective was to apply a central composite design to develop the simvastatin-loaded nanosponge formulation to improve its oral bioavailability. Methods: With the help of a design expert (State-Ease version 13.0.1), a central composite design was selected for the formulation of simvastatin-loaded nanosponges by using a defined concentration of Eudragit L-100 (X1) and PVA (X2) as independent variables and particle size (Y1), percent (%) entrapment efficiency (EE) (Y2), in vitro drug release (Y3) as dependent variables. Fourteen (SF1-SF14) formulations were prepared using the emulsion solvent evaporation and evaluated for surface morphology, particle size, drug-excipient compatibility, %EE, and % drug release. The optimized model (SF14) obtained from a design expert was evaluated for in vivo pharmacokinetics in animal models. Results: SF14 was formulated and evaluated for morphology (shape and size) of the particle, % EE, in vitro % drug release, and its kinetics. The formulation showed particle size of 163±0.45 nm, 80.54 %±0.57 of EE, and 97.13%±0.38 of drug release at 8h. The release kinetics followed the zero-order and Higuchi mechanisms with non-fiction diffusion. In vivo results showed Cmax, Tmax, AUC0-t, AUC0-α, and MRT0-α for nanosponges were 0.175 µg/ml, 6 h, 1.561 µg/mlh, 1.755 µg/mlh, 11.77 h, respectively. Conclusion: The results indicated a significant increase in the bioavailability of the drug in nanosponges compared with standard drugs. The experimentally designed nanosponge formulations have been successfully developed, and evaluated parameters show that the nanosponge formulation of Simvastatin is a promising delivery through the oral route.
Comparing Clock Test for Cognitive Screening: Quantitative Analysis of Five Scoring Methods – What Is Optimal?
Authors: Gea Pandhita  , Bambang Sutrisna  , Samekto Wibowo  
Published: September 2023

Objectives: The clock drawing test (CDT) is a widely used cognitive screening tool that has been well accepted. However, it is still questionable which scoring system is the best. We assessed the correlation of five common scoring methods of the CDT with Mini Mental State Examination (MMSE), and intercorrelations between each different scoring system.
Methods: CDT scores of different scoring systems from participants from the geriatric club in Pondok Kopi Jakarta Islamic Hospital, were compared. The CDT was scored according to the methods of Rouleau, Babins, Mendez, Lin, and Shulman. Correlation coefficients between the five scoring methods of the CDT with MMSE were calculated, as well as the intercorrelations between each different scoring system.
Results: The study sample consisted of 106 non-demented subjects, aged between 60 and 77 years. The five scoring methods of the CDT correlated significantly with MMSE, but correlation coefficients were moderate (r = 0.43 to r = 0.46). The highest was Lin's method (r = 0.46). Especially, correlation coefficients between the five scoring methods of the CDT with MMSE attention domain were higher (r = 0.42 to r = 0.52). The intercorrelations between each different scoring system were high (r = 0.87 to r = 0.99). The correlation coefficients between Rouleau's method with Mendez's method was the highest (r = 0.99).
Conclusions: The correlation of five common scoring methods of the CDT with MMSE were moderate positive. The CDT scored according to the method of Lin was the highest. The intercorrelations between each different scoring system were high.
Analisis Kadar Nrf2 plasma Pada Tikus dengan Defisisensi Vitamin B12
Authors: Stujanna Endin Nokik , Ujianti Irena , Mustifah Etty Farida 
Published: September 2023

Background. Vitamin B12 deficiency is a significant problem, particularly in developing countries. The increasing complexity of the Vitamin B12 molecule, the deficiency condition, causes a complex cell level disorder. The clinical manifestation of vitamin B12 deficiency is anemia, but it is believed that due to the complexity of vitamin B12, there are still other molecular disorders. This study aims to establish the pathway of damage caused by vitamin B12 deficiency in the Nrf2 molecule.
Methods. An animal experiment involving 12 male Sprague Dawley rats were randomly divided into control and 16-week treatment. There are two research groups, namely the control group and the treatment group. The control group was given a standard diet, while the treatment group was assigned a vitamin B12 deficiency diet. At the end of treatment, The levels of vitamin B12 in the liver tissue and plasma NRF2 were measured.
Statistical Analysis. Nrf2 and vitamin B12 levels were statistically analyzed using the Student's T-test, and the correlation between vitamin B12 and Hb was analyzed using the Spearmen test. Data that cannot use by Independent T-test were tested using the Mann Whitney test.
Results/Conclusion. There was no significant difference between the control and treatment groups on plasma Nrf2 levels (4.02 ± 0.59 pg/ml vs 3.34 ± 0.55 pg/ml; p = 0.07) and tissue vitamin B12 (0.0035 ± 0.0016 µg/ml vs 0.0030 ± 0.0006 µg/ml ; p = 0.8).
Keywords: Nrf2 Plasma, Defisiensi, Vitamin B12
Gambaran Hematologi pada Defisiensi Vitamin B12
Authors: Ujianti Irena 
Published: September 2023

Background and Aims. Anemia is a condition when the number of red blood cells or hemoglobin is lower than the normal number. The main cause of anemia is a lack of factors for the formation and maturation of red blood cells. Vitamin B12 plays a role in the synthesis and maturation of red blood cells, a lack of this vitamin will cause the formation and maturation of red blood cells to be disrupted.
Method. This was an experimental animal study with twelve rats with frederer's family. The rat was fed a Vitamin B12 Deficient Diet for 16 weeks as the treatment group and standard diet as the control group. Vitamin B12 in the liver was analyzed in rats for 16 weeks. Hemoglobin and MCV were analyzed in plasma for 16 weeks. Results are expressed as means of control, and test animals were established by ANOVA using SPSS 20.0 software
Results. In the present study, we found that liver vitamin B12 level was decreased in treatments groups compares to the positive control group at sixteen weeks (p<0.01). Hemoglobin was decreased in the treatment group compare positive control group at sixteen weeks (p<0.001). Mean Corpuscular Volume (MCV) was increased in the treatment group's compare positive control group at sixteen weeks (p<0.01)
Conclusions. Our findings demonstrate that vitamin B12 deficiency decreased hemoglobin and increased MCV.
Difficulties Differentiating Between Basal Cell Carcinoma and Trichoepithelioma: A Case Report
Authors: Nurusshofa Zahra , Irena ujianti  
Published: September 2023

Background. Basal cell carcinoma (BCC) is the most common type of malignant skin tumor with basal cell differentiation. Trichoepithelioma (TE), however, is a rare benign skin tumor with follicular differentiation. Both types of tumor have commonalities in terms of clinical and histopathological features. Some cases, in fact, require ancillary studies to distinguish between the two. Confusing the two tumors may result in different future outcomes for the patient, due to their vastly different prognoses and treatments. This case report outlines two different cases, and discusses how to distinguish between the two types of tumor without the use of an ancillary study.Method. We examined the cases of one patient with basal cell carcinoma and one patient with trichoepithelioma, comparing their clinical manifestations and characteristic histological features.Results. Trichoepithelioma and BCC share several overlapping histopathological features. Trichoepithelioma and BCC share several overlapping histopathological features. The TE in this case report also exhibited a nodular pattern, connection to the epidermis, stromal cleft, and prominent nuclear palisading, which are more commonly found in BCC. However, scanty mitotic activity, the presence of non-atypia cells, and symmetry of the lesion, are favor TE.Conclusion. Differentiation between BCC and TE is only possible using cytomorphological assessment with a high-power field of view.
PENATALAKSANAAN KOMPREHENSIF LANSIA DENGAN PENURUNAN FUNGSI KOGNITIF DAN DEMENSIA
Authors: Pandhita S Gea , Laksmi Purwita W , Marfianti Erlina 
Published: September 2023

Pasien Geriatri mempunyai karakteristik yang khusus, berbeda dengan karakteristik pasien pada golongan usia yang lain. Pasien geriatri antara lain ditandai dengan karakteristik memiliki multi-morbiditas atau multi-penyakit, kapasitas fisiologis yang menurun, manifestasi klinis penyakit yang tidak khas, status fungsional menurun, dan malnutrisi. Kondisi ini akan menimbulkan masalah kesehatan spesifik yang lazim ditemui pada pasien geriatri, yaitu: Frailty, Sarkopenia, Delirium, Jatuh, Gangguan Tidur, Dizziness, Syncope, Ulkus Dekubitus, Inkontinensia, dan Elder mistreatment. Beberapa masalah kesehatan yang sering dialami oleh pasien geriatri adalah Instability, Immobility, Infection, Incontinence, Intellectual impairment (Mild/Vascular Cognitive Impairment, Dementia), Impairment of hearing & vision, Impaction (konstipasi), Isolation (depresi), Inanition (malnutrisi), Impecunity (kemiskinan), Iatrogenic, Insomnia, Immune deficiency, dan Impotence. Berbagai syndrome geriatri ini saling berinteraksi secara kompleks. Oleh karena itu perlu pendekatan khusus dalam penilaian dan penatalaksanaan kasus geriatri,. Pendekatan ini sering dikenal sebagai Comprehensive Geriatric Assessment (CGA).
Chronic Limb Ischemia: Awareness and Treatment in Primary Care
Authors: Purwowiyoto Sidhi Laksono , Halomoan Reynaldo 
Published: September 2023

Chronic limb ischemia (CLI) is a type of peripheral arterial disease (PAD) that is still underdiagnosed and undertreated despite the increasing incidence, thus becoming a global health burden. The prevalence of PAD is quite high, approximately around 200 million globally in the adult population and increased drastically in older age population. It might be difficult to diagnose at an early stage because around 20-50% patients may be asymptomatic. More than 70% of primary physicians also did not notice that the diagnosis was already established during screening of the PAD patients. All these problems could increase the incidence of PAD in the following year. The untreated conditions will develop into a more severe form of PAD known as chronic limb-threatening ischemia (or critical limb ischemia), and patients are at a higher risk of having limb loss, and also increased morbidity and mortality. The primary physicians in the primary health facilities hold an important role in the early diagnosis and management of patients with CLI symptoms or with risk factors of CLI. Due to the limitation of diagnostic testing modality at primary health facilities, the physician can assess the ankle-brachial index (ABI) to determine the presence of CLI. Management of the disease is different for every patient and is customized based on the other comorbidities. Risk factors should be controlled in order to achieve a better outcome. A good management strategy will improve the condition. This review aims to describe how to give an early diagnosis and management for CLI patients in primary health care.
Korelasi Lama Menjalani Hemodialisis Dengan Kualitas Hidup Pada Penderita Nefropati Diabetik Di RS Dinda Tangerang Dalam Periode 3 Tahun
Authors: Nugraha Dhiki , Hasan Fahad , Gunawandjati Dedy , Purwowiyoto Sidhi Laksono , Stujana Endin Nokik , Hidayat Ahmad 
Published: September 2023

Pendahuluan: Salah satu komplikasi mikrovaskular dari diabetes yaitu nefropati diabetik. Penderita nefropati diabetik yang menjalani hemodialisis membutuhkan waktu yang lama dan membutuhkan kepatuhan dalam mengobati komorbid yang mendasarinya. Hal ini akan memberikan stresor baik fisiologis maupun psikologis penderita yang akan mempengaruhi kualitas hidup. Tujuan penelitian ini menentukan korelasi antara lamanya menjalani terapi hemodialisis pada penderita nefropatik diabetik dengan kualitas hidup di RS Dinda Tangerang.
Metode: metode penelitian menggunakan analisis parametrik dengan metode pendekatan non parametrik. sampel penelitian ini menggunakan consecutive sampling yang melibatkan 40 penderita nefropati diabetik. data di analisis menggunakan korelasi spearman. Teknik pengumpulan data menggunakan kuesioner KDQOL SF 1.3.
Hasil: berdasarkan hasil penelitian didapatkan 23 penderita 57.5% yang menjalani hemodialisis 1- 3 tahun dan 6 di antara nya penderita yang memiliki kualitas hidup kurang sebanyak 2 orang, dan yang memiliki kualitas hidup cukup sebanyak 2 orang serta sebanyak 15 orang lainya memiliki kualitas hidup baik.
Simpulan: kesimpulan penelitian ini terdapat korelasi yang bermakna p=0,043 antara lama menjalani hemodialisis dengan kualitas hidup dengan nilai (r): 0.309 yang mana mengarah ke arah positif sehingga ketika penderita telah lama melakukan terapi hemodialisis maka semakin baik kualitas hidup dari penderita.
Actuar de Enfermería en el parto: dilemas del deber ser y hacer
Authors: Amézquita-Cárdenas Luisa Fernanda 
Published: September 2023

El parto es un proceso natural, sin embargo las intervenciones medicalizadas e injustificadas hicieron que este proceso se patologizara y esa clase de actos hacen que el proceso del parto para las mujeres se conviertan en experiencias negativas, ya que, pasan por momentos donde se les vulnera su autonomía y protagonismo. Por lo que en este artículo se busca reflexionar sobre el parto humanizado desde la perspectiva de un estudiante durante sus prácticas formativas, además, de tener en cuenta las filosofías y miradas antropológicas que marcan la pauta en el actuar del profesional, el biopoder del cuerpo de una mujer en labor de parto que es sometida al paternalismo de quienes la atienden. Por otro lado, cómo la infraestructura del lugar donde se atiende a las mujeres y la falta de habilidades como lo es la empatía en el profesional de salud son determinantes durante su atención y cómo impacta en la falta de humanización.
Conocimientos, actitudes y prácticas del uso de métodos anticonceptivos en mujeres en edad reproductiva.
Authors: Rojas-Rojas Laura Alejandra , Pinto-Rosas Erika Marlen , Quemba-Mesa Monica Paola 
Published: September 2023

Objetivo: Determinar los conocimientos, las actitudes y las prácticas en el uso de métodos anticonceptivos en mujeres en edad reproductiva del barrio las Delicias de la ciudad de Duitama – Boyacá en el año 2021. Materiales y métodos: Estudio cuantitativo descriptivo – transversal, contando con una muestra calculada de 88 mujeres con muestreo a conveniencia, incluyendo a mujeres entre los 18 y 54 años, aplicando la encuesta validada “Conocimientos, actitudes y prácticas sobre los métodos anticonceptivos” y el análisis estadístico fue de tipo univariado por medio del Software SPPS versión 23. Resultados: Las participantes tenían una edad promedio de 30.67 años (DE: 9,5), la mayoría tenía como estado civil ser soltera (48%; n: 43) y acudían a los programas de planificación familiar (71,6%; n: 63). Todas manifestaron conocer los métodos anticonceptivos (100%; n:88), sin embargo, el 40% (n:36) no tiene claridad sobre las reacciones adversas de la píldora; además el 43,1% (n:38) consideran que al usar el preservativo se disfruta menos de la relación sexual. En mujeres jóvenes predomina la preferencia de métodos de corta duración y hormonales, a diferencia de mujeres con mayor edad en donde predominaron los métodos larga duración o definitivos. Conclusiones: Los profesionales de la salud juegan un papel importante en la asesoría en planificación familiar al detectar las necesidades de la mujer, fortaleciendo la educación en derechos sexuales y reproductivos, para que esta población logre el disfrute de su vida sexual al adquirir una educación integral y con acceso a los métodos anticonceptivos.
Mortalidad infantil en Antioquia durante el año 2021: hacia un enfoque sindemico
Authors: García-Uribe John Camilo , Chavarría-Ramírez Vanessa Catherine 
Published: September 2023

En Colombia, la mortalidad infantil ha seguido una tendencia marcada al descenso en los últimos años. Sin embargo, tras la llegada del coronavirus se ha identificado un discreto aumento en los casos de mortalidad infantil. El objetivo es describir los factores relacionados con la mortalidad infantil y las estrategias implementadas durante el 2021. Materiales y métodos: estudio descriptivo retrospectivo, sobre casos confirmados de mortalidad infantil en Antioquia durante el 2021, las fuentes información fueron los reportes de notificación epidemiológica y las transcripciones de las entrevistas de campo de análisis de los eventos. Para el análisis de las fichas epidemiológicas se utilizaron estadísticos descriptivos y para las entrevistas análisis de contenido cualitativo. Resultados: En Antioquia, para el año 2021, se presentaron 43 muertes en menores de 5 años por Insuficiencia Respiratoria Aguda (IRA) , Enfermedad Diarreica Aguda (EDA) y Desnutrición (DNT), de los cuales la mayor incidencia la aportó la mortalidad por IRA con 24 casos, le sigue la mortalidad por desnutrición con 10 casos y por último, la mortalidad por enfermedad diarreica aguda presentó 9 casos. Se analizaron 10 entrevistas a madres y familiares de los menores fallecidos se identificaron las siguientes categorías de análisis sindemia y mortalidad; salud mental materna y mortalidad infantil; y calidad de la atención en salud. Con base en los problemas identificados se desarrollaron dos eventos educativos con enfoque de AIEPI comunitario. Conclusión: Un enfoque de sindemia, posibilita comprender el impacto de la pandemia en la mortalidad infantil, entender es el primer paso para intervenir. El aumento de las cifras de mortalidad infantil es solo la punta del iceberg, es necesario considerar otros efectos a largo plazo en la salud infantil.