bluuebunny/crossref_metadata_2025_split huggingface dataset:
THE NEED TO DEVELOP THE PSYCHOPHYSICAL QUALITIES
OF STUDENTS IN THE CONTEXT OF FUTURE WORK
Authors: VASHKEVICH K. , SALIKOV V. 
Published: June 2023

In the article, the authors analyze the need for the development of psychophysical qualities in students in the
context of future work in their chosen profession, assess the direction of the influence of sports on the formation of professionally
significant skills, compiled professionograms and sportograms, Determine the main advantages of using
sports included in the training program in institutions of higher education and varieties of fitness for the directed development
of psychophysical qualities students. In the course of the study, the authors identify the most accessible and
popular types of sports and fitness among students for the development of important psychophysical qualities in students
in the context of their future professional activities.
THE COURSE "EDUCATION IN THE CONTEXT OF MODERN SOCIO-ECONOMIC PROCESSES"
AS A RESOURCE FOR THE DEVELOPMENT OF THE PROFESSIONAL-PEDAGOGICAL WORLDVIEW
IN MASTER’S STUDENTS
Authors: CHEKINA A. , CHEKH O. 
Published: June 2023

The article provides definitions of the concept of “professional-pedagogical worldview”, proposed by various
researchers in a very small number of works devoted to the study into that intra-personal phenomenon. Based on the analysis
of the definitions, the authors conclude that the content of the teacher's professional training should cover not only the
psychological, pedagogical, methodological and technological aspects of future professional activity, but also the socioeconomic,
legal and political aspects and the grounds for integrating their professional activity into the modern world picture.
According to the authors of the article, a significant resource for the development of the professional and pedagogical
worldview in Master’s students may be the course “Education in the context of modern socio-economic processes”. The
main tasks of teaching students the course are to master the knowledge about the main directions of the functioning of
education in the context of world and national socio-economic realities and prospects, which implies an understanding of
the essence of education as a social practice, the situation of the development of the education system in their own country
and in the world; modern approaches to the education economics and legal regulation for education, key elements of the
economic mechanism of the education sector, its socio-economic effectiveness, as well as the role of the teacher and the
results of teachers’ professional activities for the social and innovative development of society and the state. The article
presents an approximate thematic plan of the course and describes some of its content and methodical aspects.
Implementasi Manajemen Strategis Kepala Madrasah Dalam Menumbuhkan Sikap Keberagamaan Peserta Didik
Authors: Sulhan M , Lessy Zulkipli , Komsatun Kusnul 
Published: June 2023

Penelitian ini bertujuan untuk menganalisis bentuk manajemen strategis kepala madrasah dalam menumbuhkan sikap keberagamaan peserta didik. Pendekatan yang digunakan ialah deskriptif kualitatif, proses perolehan data berdasarkan hasil wawancara, observasi, dan teknik dokumentasi. Adapun analisis data menggunakan teknik reduksi data, penyajian data, dan pengambilan kesimpulan. Temuan yang diperoleh dalam penelitian mendeskripsikan langkah-langkah kebijakan dan upaya strategis kepala madrasah melalui beberapa langkah aplikatif di antaranya adalah pengamatan lingkungan, perencanaan, penyusunan kurikulum, implementasi dan evaluasi kebijakan, serta program-program intra- dan ekstrakurikuler. Analisis jalur menunjukkan bahwa manajemen strategis kepemimpinan memiliki pengaruh terhadap perubahan variabel sikap keberagamaan.
FATTY ACID COMPOSITION AND PROPERTIES OF CAMEL MILK OIL
Authors: БЕЙСЕМБЕКОВА Г.Қ. , ҚАНАЯТ Ш. , НАРМУРАТОВА М.Х. 
Published: June 2023

Мақалада түйе сүті табиғи функционалды өнім ретінде сипатталады, оның химиялық құрамы, физикалық қасиеті басқа жануарлар сүтінен ерекшелейді. Сүт құрамында адам ағзасында синтезделмейтін маңызды полиқанықпаған май қышқылдарының, триацилглицеридтердің, диацилглицеридтердің, көптеген фосфолипидтердің биологиялық құндылығы және антиканцерогенді, антимикробтық, қабынуға қарсы және иммуносупрессивті қасиеттері расталды. Бүгінгі таңда инфекциялық аурулардың өршуі және антибиотиктерге қарсы микроорганизмдердің төзімділігінің артуына байланысты, май қышқылдарының антимикробтық белсенділігін ауруларды емдеуде және профилактикада қолдануда таптырмас өнім ретінде ұсынылады. Бұл жұмысты зерттеудің мақсаты – түйе сүтін емдік мақсатта қолдану мүмкіндіктері және май қышқылдық құрамының ерекшеліктері туралы әдебиет деректерін талдау.
Milk is considered one of the most important food products for humans and animals and acts as a complete food due to the presence of important components in it, such as carbohydrates, proteins, fats, vitamins and minerals. The main energy component in the composition of milk is milk fat, which determines the economic value, special taste and physico-chemical properties of dairy products. Milk fat is an indispensable source of energy, fat-soluble nutrients and bioactive lipids for mammals, as well as an important medium for the delivery of nutrients, including fat-soluble vitamins. Bioactive lipids in milk contain essential polyunsaturated fatty acids that are not synthesized in the human body, such as short-chain fatty acids, triacylglycerides, diacylglycerides, many phospholipids and fat-soluble vitamins. The benefits of milk lipids include antitumor, antimicrobial, anti-inflammatory and immunosuppressive properties.
The article describes camel milk as a natural functional product, it is chemical composition and physical properties differ from the milk of other animals. Milk has confirmed the biological value of essential polyunsaturated fatty acids, triacylglycerides, diacylglycerides, many phospholipids and anti-carcinogenic, antimicrobial, anti-inflammatory and immunosuppressive properties that cannot be synthesized in the human body. Today, due to the development of infectious diseases and increased resistance of microorganisms to antibiotics, antimicrobial activity of fatty acids is recommended as an indispensable product in the treatment and prevention of diseases. The purpose of this study is to analyze the literature data on the possibilities of using camel milk for medicinal purposes and the features of its fatty acid composition.
SPECTRUM OF VOLATILE COMPOUNDS OF THE PROBIOTIC CONSORTIUM FOR FISH IN AQUACULTURE
Authors: ЧИЖАЕВА А.В. , АЛИМЖАНОВА М.Б. , АШИМУЛЫ Қ. , САДАНОВ А.К. , АЛЫБАЕВА А.Ж. , ЕРМЕКБАЙ Ж.Н. , АМАНГЕЛДІ А.А. , ЕЛУБАЕВА М.Е. 
Published: June 2023

Летучие органические соединения представляют интерес во многих областях. Среди них анализ продуктов питания и ароматизаторов, исследования окружающей среды и атмосферы, промышленное применение, безопасность или медицина и наука о жизни. Характеристика этих соединений в основном выполнялась путем сбора образцов и анализа за пределами предприятия с помощью газовой хроматографии в сочетании с масс-спектрометрией в качестве золотого стандарта. С целью определения химической природы метаболитов консорциума антагонистически активных микроорганизмов Lacticaseibacillus paracasei Wf-2, Limosilactobacillus pontis Wf-6, Lacticaseibacillus casei Wf-10, Lacticaseibacillus paracasei Wf-20 и Propionibacterium freudenreichii P-8 был исследован спектр летучих соединений консорциума с применением газовой хроматографии с масс-спектрометрическим детектированием. Обнаружены метаболиты, обладающие антибактериальной и противогрибковой активностью – органические кислоты, ароматические кислоты (префеновая кислота, гидроксифениллактат, пирролидонкарбоновая кислота и полимолочная кислота), уксусная кислота и другие летучие вещества, а именно диацетил, ацетоин и др.
Volatile organic compounds are of interest in many fields. These include food and flavor analysis, environmental and atmospheric research, industrial applications, safety or medicine, and life sciences. The characterization of these compounds was primarily performed by off-site sampling and analysis using gas chromatography combined with mass spectrometry as the gold standard. In order to determine the chemical nature of the metabolites of the consortium of antagonistically active microorganisms Lacticaseibacillus paracasei Wf-2, Limosilactobacillus pontis Wf-6, Lacticaseibacillus casei Wf-10, Lacticaseibacillus paracasei Wf-20 and Propionibacterium freudenreichii P-8, the spectrum of volatile compounds of the consortium was studied using gas chromatography with mass spectrometric detection. Metabolites with antibacterial and antifungal activity were found - organic acids, aromatic acids (prefenic acid, hydroxyphenyl lactate, pyrrolidonecarboxylic acid and polylactic acid), acetic acid and other volatile substances, namely diacetyl, acetoin, etc.
TECHNOLOGY OF PRODUCTION OF BAKERY PRODUCTS MADE OF TRITICALE FLOUR USINGA STARTER CULTURE BASED ON LACTIC ACID BACTERIA
Authors: УМИРАЛИЕВА Л.Б. , АБУОВА А.Б. , КАНДРОКОВ Р.Х. , ИСАБЕКОВА М.С. , ХАЛМУХАМЕДОВА М. 
Published: June 2023

Пшенично-ржаной гибрид - тритикале, обладая богатым аминокислотным составом и пониженным содержанием глютена в составе муки, на сегодняшний день является перспективной зерновой культурой для использования в хлебопекарном производстве. Однако традиционные рецептуры, которые применяются для выпечки пшеничного и ржаного хлеба, не подходят для тритикалевого, требуя нового подхода к технологии приготовления. Для улучшения свойств теста и готовой выпечки использована закваска на основе консорциума молочнокислых бактерий. В состав консорциума вошли три штамма лактобактерий: Limosilactobacillus fermentum 3Ш1, Limosilactobacillus pontis 9K3, Lacticaseibacillus paracasei 126. На основе консорциума были разработаны несколько видов закваски, содержащих тритикалевую и пшеничную муку, воду и консорциум. Были определены кислотность, антагонистическая и ферментативная активность закваски. Осуществлена пробная выпечка хлеба с разным содержанием закваски, в ходе которой было определено, что добавление закваски в количестве 10% в технологическом процессе выработки тритикалевого хлеба позволяет сократить продолжительность расстойки на 12 минут, улучшает качество готового продукта и увеличивает сроки хранения готовых изделий до 5-6 суток, а также повышает устойчивость хлеба к «картофельной болезни» и плесневению за счет высокой антагонистической активности пробиотических микроорганизмов закваски.
Wheat-rye hybrid triticale, having a rich amino acid composition and a reduced gluten content in flour, is currently a promising grain crop for use in bakery production. However, traditional recipes that are used for baking wheat and rye bread are not suitable for triticale, requiring a new approach to cooking technology. To improve the properties of the dough and the finished baking, a starter culture based on a consortium of lactic acid bacteria was used. The consortium includes three strains of lactobacilli: Limosilactobacillus fermentum 3Н1, Limosilactobacillus pontis 9K3, Lacticaseibacillus paracasei 126. Based on the consortium, several types of starter cultures containing triticale and wheat flour, water, and the consortium were developed. The acidity, antagonistic and enzymatic activity of the starter were measured. A trial baking of bread with different sourdough content was carried out, during which it was determined that the addition of sourdough in an amount of 10% in the technological process of producing triticale bread reduces the duration of proofing by 12 minutes, improves the quality of the finished product and increases the shelf life of finished products up to 5-6 days, and also increases the resistance of bread to "potato disease" and mold formation due to the high antagonistic activity of probiotic microorganisms of the starter culture.
Mensajes furtivos
Authors: García de Vicuña Gortazar Harriet 
Published: June 2023

El texto reflexiona sobre la paradoja implícita en las cartas codificadas. Dicha incongruencia es consecuencia del problema de la apertura de la escritura, es decir, de la imposibilidad del escrito de discriminar a sus lectores: un texto está expuesto a todos los ojos.
La paradoja se acentúa cuando el escrito tiene un destinatario concreto y determinado: la intención, la necesidad de excluir de la lectura del texto a todos aquellos que se consideran inadecuados obliga al remitente a enfrentarse al siguiente problema: ¿Cómo escribir un texto que discrimine de manera selectiva, es decir, que “elija” al receptor adecuado?
La reflexión sobre este tema se orienta mediante la exposición y el análisis de ejemplos en los que el mantenimiento del secreto es una cuestión de vida o muerte: fallar en la codificación del escrito puede llevar al remitente a la muerte, la cárcel o el exilio. La indiscreta apertura del texto se convierte en el problema central a la hora de analizar las prácticas de comunicación, en el contexto de las organizaciones que hacen del secreto fundamento del propio existir.
Analysis of immune response of stressed male mice during Chlamydia muridarum lung infection
Authors: Woart Nathasha 
Published: June 2023

Chlamydia lung infection caused by Chlamydia trachomatis is a serious lung infection particularly in infants but there are a few studies about lung infection. Chlamydial genital and lung infections in mice is frequently done with Chlamydia muridarum. The purpose of our study was to explore the effect of stress in lung chlamydia infection in male mice. We hypothesize that stress changes the level of cytokine production in monocytes during lung infection. As a The result from these studies include counting of live cells, differentiation of proliferation of bone marrow derived monocytes where the result showed that non-stressed dendritic cells (DCs) WT mice and stressed macrophages (MO) WT mice had sufficient live cells to study. No difference in IL-b production of macrophages of stressed and non-stress mice was observed. Beta2-adrenergic receptor (b2-AR agonist (fenoterol) and antagonist (ICI 118 551) treatment resulted in difference in cytokine production the effect of agonists and antagonists. TNF-alpha production was high in stressed mice compared to non-stress mice for MO. LPS stimulated TNF-alpha production in DC but showed no difference in stressed and non-stressed mice. However, TNF-alpha production in macrophages of non-stressed mice was decreased. The production of IFN-g, in Con A- and LPS-treated splenic T cells. In contrast IL-5, IL-10, and IL-23 production was high in T cells of stressed mice. T cells treated with norepinephrine and bet2-adrenergic receptor agonist, Fenoterol. Overall, the production of cytokines in stressed and non-stressed mice shows variation that may have roles in enhancing protection or increased lung infection.
Identification by DNA barcode of viable fungi captured in a teaching laboratory in West Virginia
Authors: Harsh Robert , Robertson Laura 
Published: June 2023

Fungi are ubiquitous and their spores are found in the air outside and within the built environment. We investigated the fungal species present in a teaching laboratory at Shepherd University. Airborne fungi were captured passively at three sites within the teaching laboratory on two different culture media. Viable fungi were isolated to pure culture and then identified by DNA barcode using the internal transcribed spacer region of the ribosomal DNA. Twenty-four isolates were identified to order, family, genus, or species; most isolates were not resolved to species by DNA barcode. Captured fungi belonged to Helotiales (1 isolate), Pleosporales (2 isolates), Hypocreales (1 isolate), Didymellaceae (1 isolate), Cladosporiaceae (5 isolates), Aspergillaceae (1 isolate), Sporocadaceae (1 isolate), Peniophora (1 isolate), Trichoderma (1 isolate), Penicillium (4 isolates), Fusarium (2 isolates), and Myrmecridium (1 isolate). Three fungal isolates were identified as Xenoacrodontium juglandis. These results are similar to other studies investigating the fungal community in the built environment, where Cladosporium, Penicillium, and Aspergillus are the most commonly identified genera.
Deletion of FTL_1199 to determine the role of this gene in erythrocyte invasion by Francisella tularensis.
Authors: Delatore III Elio , Horzempa Joseph , Cantlay Stuart , Roberts Elle 
Published: June 2023

Francisella tularensis is a bacterium that induces the zoonotic disease tularemia. In the course of infection, F. tularensis bacteria invade erythrocytes, a phenomenon that heightens the colonization of ticks after a blood meal. To better understand the mechanism of erythrocyte invasion, we hypothesized that transcription of bacterial genes significant in erythrocyte invasion would be upregulated upon exposure to these host cells. An RNA-seq unveiled that transcription of 7% of F. tularensis genes augment when in erythrocyte presence. Of these, we pinpointed a putative transcriptional regulator, FTL_1199. The goal was to delete FTL_1199 in F. tularensis LVS. SOE PCR amplified and duplicated the up and downstream regions of the target gene in tandem into a shuttle vector that is insecure within F. tularensis. This newly generated plasmid, pDEL1199, was mobilized inside of F. tularensis by conjugation. Merodiploid strains generated by homologous recombination were isolated and transformed with pGUTS. Expression of I-Sce1 within the merodiploid produces a double-stranded break. This breakage resulted in a second recombination that either ensued to wild-type or deletion of FTL_1199 deduced through a PCR. Finally, in DFTL_1199 strains, pGUTS was cured by successive cultivation in the absence of selection followed by replica-plating on chocolate II agar ± kanamycin. Gentamicin protection assays showed reduced levels of erythrocyte invasion for F. tularensis DFTL_1199 compared to wild type bacteria. However, complementation of FTL_1199 to the deletion mutant restored this strain’s ability to invade red blood cells. These findings demonstrate that FTL_1199 is important for erythrocyte invasion by F. tularensis.

(Supported by NIH Grant P20GM103434 to the West Virginia IDeA Network for Biomedical Research Excellence, R15HL14735 from NHLBI, and funds from the NASA West Virginia Space Grant Consortium).
Analyzing the contribution to pathogenesis by three putative transcriptional regulators of Francisella tularensis using the chicken embryo infection model.
Authors: Shania Davis  
Published: June 2023

SHANIA DAVIS, MARIAH CASHBAUGH, ELIO DELATORE & JOSEPH HORZEMPA Dept of Biomedical Sciences, West Liberty University, West Liberty, WV, 26074. Analyzing the contribution to pathogenesis by three putative transcriptional regulators of Francisella tularensis using the chicken embryo infection model.
Francisella tularensis is a highly pathogenic bacterium that can be used as a biological weapon. This bacterium causes tularemia which is also known as Rabbit Fever. Previously, our laboratory identified three F. tularensis loci predicted to encode transcriptional regulators (FTL_0671, FTL_1199, and FTL_1665) that exhibited transcriptional changes in the presence of human erythrocytes. We hypothesized that because transcriptional modulations were associated with the presence of host cues, these potential transcriptional regulators may be important for pathogenesis. Therefore, mutants of these three loci and the isogenic wild-type bacterium (strain LVS) were used to infect chicken embryos. Healthy chicken embryos were injected with 104, 105, or 106 colony forming units of each strain, and chicken embryoys were candled daily for viability. The chicken embryos were housed in an egg incubator (~37°C with occasional rocking) and were observed over a span of 10 days. Neither FTL_0671 nor FTL_1199 affected the pathogenesis of F. tularensis. However, chicken embryos infected with the FTL_1665 deletion strain exhibited mortality more rapidly compared to those infected with wild-type bacteria suggesting that this locus may be repressing pathogenesis. Future studies will evaluate the function of FTL_1665 to determine if the protein encoded by this locus truly mediates transcriptional regulation.
The Efficacy, Toxicity & Pharmacology of Dillapiole: a potential new treatment for Tularemia
Authors: Delatore III Elio , Horzempa Joseph , Clark Zachary , Ayala Megan 
Published: June 2023

Francisella tularensis, a highly infectious bacterium, is the causative agent of Tularemia (rabbit fever). Categorized by the Center for Disease Control and Prevention as a Category A bioterrorism agent, Francisella tularensis is of the highest level of concern. Previously, we identified that dillapiole, a compound extracted from fennel, dampens F. tularensis virulence gene expression. While having no apparent effect on the viability of F. tularensis, treatment with this compound leads to reduced bacterial viability during in vitro infection of THP-1 monocytes and RAW 264.7 macrophages. In this study, we sought to determine if dillapiole exhibited a therapeutic effect in vivo, and to characterize the toxicity and pharmacology of this compound. In a murine tularemia model, female mice treated with dillapiole trended toward increased survival compared to those treated with the vehicle. However, dillapiole- or vehicle-treated male mice showed increased mortality compared to the females, suggesting gender-specific differences in the murine immune response to F. tularensis. Dillapiole was not toxic to HEK-293 cells in vitro, nor was this compound toxic to primary human hepatocytes when tested up to a concentration of 11 mg/ml (50 mM). Dillapiole was shown to be relatively stable in human, rat, and mouse plasma with a half-life greater than 120 minutes in all cases. However, this compound showed moderately high binding to plasma proteins (86% in human plasma and 75% in mouse plasma). In addition, while dillapiole showed moderate clearance by human and rat liver microsomes, mouse liver microsomes exhibited high clearance. Collectively, these data could explain the minimal efficacy observed in vivo. Therefore, future investigations should involve the rat infection model to determine the potential efficacy of dillapiole as a novel treatment for tularemia.
Taxonomic Standing and Genetic Surveillance of the Spiny Scale Crayfish (Cambarus jezerinaci) in Southeastern Kentucky
Authors: Gebhardt Cassidy , Loughman Zachary 
Published: June 2023

In Southeastern Kentucky a small, cold stream-dwelling crayfish, Cambarus jezerinaci, located in the Kentucky and Cumberland River basin may be a part of a species complex. A previous study done in 2008 on the cytochrome oxidase 1 gene (CO1) of C. jezerinaci and Cambarus parvoculus, a sister species in the region, identified a third genetically distinct lineage most similar to C. jezerinaci currently retained as Group B C. jezerinaci. Group B is as different from the true species as the true species is to C. parvoculus, a total CO1 difference greater than 5%. Morphological characteristics were also compared between C. jezerinaci and C. parvoculus with only rostral states being significant to species identification. However, in light of new genetic lineages previous research suggested the separation of them before morphological comparisons. In 2022 and 2023 research was conducted on both the genetics and morphological differences of C. jezerinaci within two river basins to better understand the taxonomic standing of this crayfish. Determining the true status of C. jezerinaci within these two river basins is important to the conservation of these animals. Continuing genetic research helps us to grow our understanding of what it means to be a species in the modern age and also allows us to learn about the biodiversity and speciation process itself. Given the rapidly changing climate, it is imperative to catalog the true diversity of our freshwater streams and develop conservation action plans for endemic species such as C. jezerinaci.