bluuebunny/crossref_metadata_2025_split huggingface dataset:
Lack of IFN-γ Receptor Signaling Inhibits Graft-versus-Host Disease by Potentiating Regulatory T Cell Expansion and Conversion
Authors: Zhai Naicui , Liu Wentao , Jin Chun-Hui , Ding Yanan , Sun Liguang , Zhang Donghui , Wang Zhaowei , Tang Yang , Zhao Wenjie , LeGuern Christian , Mapara Markus Y , Wang Hui , Yang Yong-Guang 
Published: July 2023

Abstract
IFN-γ is a pleiotropic cytokine that plays a controversial role in regulatory T cell (Treg) activity. In this study, we sought to understand how IFN-γ receptor (IFN-γR) signaling affects donor Tregs following allogeneic hematopoietic cell transplant (allo-HCT), a potentially curative therapy for leukemia. We show that IFN-γR signaling inhibits Treg expansion and conversion of conventional T cells (Tcons) to peripheral Tregs in both mice and humans. Mice receiving IFN-γR–deficient allo-HCT showed markedly reduced graft-versus-host disease (GVHD) and graft-versus-leukemia (GVL) effects, a trend associated with increased frequencies of Tregs, compared with recipients of wild-type allo-HCT. In mice receiving Treg-depleted allo-HCT, IFN-γR deficiency–induced peripheral Treg conversion was effective in preventing persistent GVHD while minimally affecting GVL effects. Thus, impairing IFN-γR signaling in Tcons may offer a promising strategy for achieving GVL effects without refractory GVHD. Similarly, in a human PBMC-induced xenogeneic GVHD model, significant inhibition of GVHD and an increase in donor Tregs were observed in mice cotransferred with human CD4 T cells that were deleted of IFN-γR1 by CRISPR/Cas9 technology, providing proof-of-concept support for using IFN-γR–deficient T cells in clinical allo-HCT.
The NQR Complex Regulates the Immunomodulatory Function of Bacteroides thetaiotaomicron
Authors: Engelhart Morgan J , Glowacki Robert W P , Till Jessica M , Harding Clifford V , Martens Eric C , Ahern Philip P 
Published: July 2023

Abstract
The gut microbiome and intestinal immune system are engaged in a dynamic interplay that provides myriad benefits to host health. However, the microbiome can also elicit damaging inflammatory responses, and thus establishing harmonious immune–microbiome interactions is essential to maintain homeostasis. Gut microbes actively coordinate the induction of anti-inflammatory responses that establish these mutualistic interactions. Despite this, the microbial pathways that govern this dialogue remain poorly understood. We investigated the mechanisms through which the gut symbiont Bacteroides thetaiotaomicron exerts its immunomodulatory functions on murine- and human-derived cells. Our data reveal that B. thetaiotaomicron stimulates production of the cytokine IL-10 via secreted factors that are packaged into outer membrane vesicles, in a TLR2- and MyD88-dependent manner. Using a transposon mutagenesis-based screen, we identified a key role for the B. thetaiotaomicron–encoded NADH:ubiquinone oxidoreductase (NQR) complex, which regenerates NAD+ during respiration, in this process. Finally, we found that disruption of NQR reduces the capacity of B. thetaiotaomicron to induce IL-10 by impairing biogenesis of outer membrane vesicles. These data identify a microbial pathway with a previously unappreciated role in gut microbe–mediated immunomodulation that may be targeted to manipulate the capacity of the microbiome to shape host immunity.
PD-L1/BTLA Checkpoint Axis Exploited for Bacterial Immune Escape by Restraining CD8+ T Cell–Initiated Adaptive Immunity in Zebrafish
Authors: Hu Chong-bin , Huang Chen , Wang Jie , Hong Yun , Fan Dong-dong , Chen Ye , Lin Ai-fu , Xiang Li-xin , Shao Jian-zhong 
Published: July 2023

Abstract
Programmed death-ligand 1/programmed cell death 1 (PD-L1/PD-1) is one of the most important immune checkpoints in humans and other mammalian species. However, the occurrence of the PD-L1/PD-1 checkpoint in evolutionarily ancient vertebrates remains elusive because of the absence of a PD-1 homolog before its appearance in tetrapods. In this article, we identified, to our knowledge, a novel PD-L1/B and T lymphocyte attenuator (BTLA) checkpoint in zebrafish by using an Edwardsiella tarda–induced bacterial infection model. Results showed that zebrafish (Danio rerio) PD-L1 (DrPD-L1) and BTLA (DrBTLA) were differentially upregulated on MHC class II+ macrophages (Mϕs) and CD8+ T cells in response to E. tarda infection. DrPD-L1 has a strong ability to interact with DrBTLA, as shown by the high affinity (KD = 5.68 nM) between DrPD-L1/DrBTLA proteins. Functionally, the breakdown of DrPD-L1/DrBTLA interaction significantly increased the cytotoxicity of CD8+BTLA+ T cells to E. tarda–infected PD-L1+ Mϕ cells and reduced the immune escape of E. tarda from the target Mϕ cells, thereby enhancing the antibacterial immunity of zebrafish against E. tarda infection. Similarly, the engagement of DrPD-L1 by soluble DrBTLA protein diminished the tolerization of CD8+ T cells to E. tarda infection. By contrast, DrBTLA engagement by a soluble DrPD-L1 protein drives aberrant CD8+ T cell responses. These results were finally corroborated in a DrPD-L1–deficient (PD-L1−/−) zebrafish model. This study highlighted a primordial PD-L1/BTLA coinhibitory axis that regulates CD8+ T cell activation in teleost fish and may act as an alternative to the PD-L1/PD-1 axis in mammals. It also revealed a previously unrecognized strategy for E. tarda immune evasion by inducing CD8+ T cell tolerance to target Mϕ cells through eliciting the PD-L1/BTLA checkpoint pathway.
Molecular Mechanisms of Lupus Susceptibility Allele PBX1D
Authors: Park Yuk Pheel , Roach Tracoyia , Soh Sujung , Zeumer-Spataro Leilani , Choi Seung-Chul , Ostrov David A , Yang Young , Morel Laurence 
Published: July 2023

Abstract
Pre–B cell leukemia homeobox 1 (PBX1) controls chromatin accessibility to a large number of genes in various cell types. Its dominant negative splice isoform, PBX1D, which lacks the DNA and Hox-binding domains, is expressed more frequently in the CD4+ T cells from lupus-prone mice and patients with systemic lupus erythematosus than healthy control subjects. PBX1D overexpression in CD4+ T cells impaired regulatory T cell homeostasis and expanded inflammatory CD4+ T cells. In this study, we showed that PBX1 message expression is downregulated by activation in CD4+ T cells as well as in B cells. PBX1D protein was less stable than the normal isoform, PBX1B, and it is degraded through the ubiquitin-proteasome–dependent pathway. The DNA binding domain lacking in PBX1D has two putative ubiquitin binding sites, K292 and K293, that are predicted to be in direct contact with DNA. Mutation of K292-293 reduced PBX1B stability to a level similar to PBX1D and abrogated DNA binding. In addition, contrary to PBX1B, PBX1D is retained in the cytoplasm without the help of the cofactors MEIS or PREP1, indicating a different requirement for nuclear translocation. Overall, these findings suggest that multiple post-transcriptional mechanisms are responsible for PBX1D loss of function and induction of CD4+ T cell inflammatory phenotypes in systemic lupus erythematosus.
Clinicoradiological Profile of COVID-19–Associated Rhino-Orbital Cerebral Mucormycosis with a Focus on Computed Tomography: A Clinical Case Series and Review
Authors: Prabhakar Anuj , Bansal Sandeep , Vyas Sameer , Bhatia Vikas , Kumar Ajay , Patro Sourabha , Singh Usha , Panda Naresh , Singh Paramjeet , Bhalla Ashish , Puri Goverdhan Dutt , Ahuja Chirag Kamal 
Published: July 2023

ABSTRACT.
COVID-19–associated rhino-orbital cerebral mucormycosis (ROCM) has a rapidly evolving course with high morbidity and mortality. We describe imaging features of COVID-19–associated ROCM based on noncontrast computed tomography (NCCT). This retrospective single-center observational study included 50 patients with COVID-19 from January 1, 2021 to June 30, 2021 who subsequently developed ROCM confirmed by fungal culture studies. All patients underwent NCCT of the paranasal sinuses as the diagnostic workup. The involvement of the nasal cavity, paranasal sinuses, orbits, and intracranial cavity was identified and graded. The ethmoid sinuses were most commonly involved [right (n = 46 of 50) > left (n = 45 of 50)], followed by the maxillary, sphenoid, and frontal sinuses. Thinning and erosions of the hard palate were noted in 18% of patients (n = 9), whereas 34% (n = 17) showed dehiscence of the lamina papyracea. Retromaxillary fat stranding was noted in 68% of patients (n = 34). Severe ethmoid sinusitis was associated significantly with ipsilateral pterygopalatine fossa involvement. The extraocular muscles were involved in 64% of patients (n = 32), with 84% (n = 42) showing orbital fat stranding. Proptosis of the affected eye was seen in 66% of patients, optic nerve involvement in 52%, and irregularity of globe contour in 12% (n = 6). The cavernous sinuses were affected in 10% of patients (n = 5), with three of them having temporal infarcts. COVID-19–associated ROCM is an acute, invasive fungal disease characterized by multisinus involvement, often with orbital and intracranial extension. Bilateral involvement with rapid progression should alert one to underlying COVID-19 disease.
Case Report: Extensive Facial Cutaneous Leishmaniasis in a Neonate
Authors: Itzhaki Gabay Sapir , Valdman-Grinshpoun Yuliya , Ling Galina , Ben-Shimol Shalom , Test Gidon , Kaplan Or , Horev Amir 
Published: July 2023

ABSTRACT.
Cutaneous leishmaniasis (CL) is a skin infection caused by various species of the Leishmania parasite and is spread by the bite of an infected female sandfly. In southern Israel, CL caused by Leishmania major is endemic. Cutaneous leishmaniasis is considered a self-limiting disease, characterized by progressive, long-lasting nodulo-ulcerative skin lesions, which usually resolve in several months to years, and leads to scarring, cosmetic disfigurement, and future stigmatization. Although CL is a common disease among children, reports of CL in children younger than 1 year are rare. We present a case of extensive facial CL in an infant whose initial lesions appeared only 25 days after birth. The patient was treated with intravenous liposomal amphotericin B. Two months later, marked improvement was seen, with complete resolution of the inflammation and atrophic scar formation. To our knowledge, this is the earliest age of CL published to date.
Detection and Isolation of Escherichia coli O157:H7 in Beef from Food Markets and Fecal Samples of Dairy Calves in the Peruvian Central Highlands
Authors: Gonzales Brenda L. , Andrade Daniel A. , Valdivia Cesar A. , Ho-Palma Ana C. , Munguia Astrid , Yucra Dora , Escobedo Max , Crotta Matteo , Limon Georgina , Gonzalez Armando , Guitian Javier , Gonzales-Gustavson Eloy 
Published: July 2023

ABSTRACT.
Shiga toxin-producing Escherichia coli (STEC) O157:H7 is a food and waterborne pathogen with severe public health implications. We report the first-time isolation of this pathogen in the Central Highlands of Peru through standardized culture procedures and polymerase chain reaction (PCR). Escherichia coli strains were cultured from rectal-anal swabs from dairy calves and beef from food markets. The latex agglutination test was used to detect O157 and H7 antigens, and multiplex real-time PCR was carried out to detect virulence-related genes. The STEC O157:H7 strains were isolated from 3.5% (1/28) of beef samples and from 6.0% (3/50) of dairy calves that also carried both eaeA and stx1 genes. Therefore, this pathogen is a potential cause of food/waterborne disease in the region, and its surveillance in both livestock and their products should be improved to characterize the impact of its zoonotic transmission. From 2010 to 2020, E. coli was suspected in 10 outbreaks reported to the Peruvian Ministry of Health. Isolates from future outbreaks should be characterized to assess the burden posed by STEC O157:H7 in Peru.
Analysis of factors affecting Micro Small and Medium Enterprises using the marketplace in the New Normal
Authors: Fadhilah Najla Najmatul , Fitri Atiyah 
Published: July 2023

This study aims to analyze the factors that influence the use of the marketplace by MSMEs in Tangerang City in facing the new normal seen from (1) knowledge (2) Trust (3) Motivation (4) Technology. The object of this research is the people of Tangerang City, while the sample of this research is MSME actors. The data used are primary data obtained from survey results by distributing questionnaires to 100 respondents in Tangerang City. The data analysis technique used in this study used the binary logistic regression method with a significance level of 5%. The results of this study show simultaneously knowledge, belief, motivation, and technology. Has an influence on the use of matketplace by MSMEs in Tangerang City. Meanwhile, partially only knowledge (0.001) and belief (0.009) have a significant effect. Other variables, motivation (0.128) and technology (0.369) did not significantly influence the use of the marketplace by MSMEs in the city of Tangerang in facing the new normal era. Binary Logistic Regression, Marketplace, MSMEs, Utilization
Dampak Penerapan Elfan Bookless Library System di Dinas Perpustakaan dan Kearsipan Daerah Kabupaten Sinjai
Authors: Congge Umar 
Published: July 2023

Digital libraries are one of the government's innovative products in an effort to increase interest in literacy and provide a place of reference for the community which is expected to function better. This digital library has been implemented in several regions in Indonesia, one of which is in Sinjai Regency. the policy of implementing digital libraries in Sinjai Regency began to be implemented in 2019. This article uses qualitative research with a descriptive approach. The data obtained is then selected, presented and analyzed using the content analysis method. The results of the study show thatElfan Bookless Library System contains approximately 5000 books that can be accessed by users. The Sinjai Regional Library and Archives Service provides services to visitors by using a QR Code that can be scanned at the Sinjai Regional Library and Archives Service. In addition, the free internet network also makes it easy for visitors to access these services. However, the majority of visitors are not aware of any information on these digital library services which is presented side by side with POCADI (Digital Reading Corner).
Комп’ютерне моделювання потоків у плоских каналах
Authors: Chelabchi V.M. , Tuzova I.A. , Panchenko T.D. , Starodub V.I. , Tuzov O.V. , Chelabchi V.V. 
Published: July 2023

У статті розглядається дослідження процесів перенесення в економічному охолоджувачі повітря непрямого випарного типу. Принцип роботи пропонованого охолоджувача повітря ‒ використання ефекту випарного охолодження. Вода (прісна або морська) є робочим агентом, що витрачається. Охолоджувачі повітря випарного типу відрізняються низькою собівартістю та малими експлуатаційними витратами. Матриця охолоджувача організована системою щілинних каналів. Для виготовлення насадки використовуються дешеві, екологічно безпечні та поширені матеріали: пластини або плівки на основі поліетилену, полістиролу чи поліпропілену та пористі неткані матеріали на основі поліпропілену або целюлози (флізелін). Описано організацію комп'ютерного моделювання тепло-масоперенесення у системі плоских каналів складного (у плані) профілю за малої величини зазору, який у 15-50 разів менше ширини каналу. В якості математичної моделі приймається система рівнянь: нерозривність, Нав'є-Стокс в проекції на координатні осі, тиску і Фур'є-Кірхгофа. Для спрощення постановки завдання запропоновано прийом усереднення величини швидкості третьої координати (за зазором в каналі). Зосередження проводиться з урахуванням профілю швидкості. Таким чином, здійснюється перехід від тривимірної до двовимірної математичної моделі. Для вирішення отриманої системи рівнянь використано модифікований різницевий метод розв'язання. У цьому випадку використовується апроксимація за триточковою схемою одночасно членів рівняння з другою та першою похідними. Вирази для коефіцієнтів апроксимуючої формули виходять методом аналітичного рішення. Схема має абсолютну стійкість. Під час комп'ютерного моделювання виділяються дві задачі. Одна ‒ спільне вирішення рівнянь нерозривності та Нав'є-Стокса. Друга ‒ розрахунок поля тиску. Виконується ітераційне узгодження рішень щодо визначення полів тисків та швидкостей потоків. Проводились дослідження функціонування охолоджувача повітря, зібраного за схемою перехресного струму (схема Мунтерса). Описано організацію натурного експерименту на макеті охолоджувача повітря. Наводяться результати натурного та обчислювального експериментів, проведених авторами. Зазначається гарне узгодження результатів експериментів.
Kepemimpinan Pendidikan Masa Depan dan Kekinian Berbasis Agama, Filsafat, Psikologi dan Sosiologi;Implementasinya dalam Lembaga Pendidikan
Authors: Koswara Nandang , Aryani Wiwik Dyah , Yulianti Lia , Armiyanti Armiyanti , Lova Nova Rati 
Published: July 2023

This study aims to examine the future educational leadership model based on the perspectives of religion, philosophy, psychology, and sociology, as well as their implications in educational institutions. The locus of this research is the Prince SMPN 1 Purwakarta Jawa Barat. This study uses a qualitative approach with descriptive analysis method. Interviews were conducted directly with informants, consisting of the school principal, the vice principal, and educational staffs in the educational institution. The results of observations and documentation were obtained from various studies of documents, manuscripts, and archives related to the implementation of educational leadership at SMPN 1 Purwakarta Jawa Barat. The results of the study indicate that the educational leadership model for the future in SMPN 1 Purwakarta after direct interviews with all parties involved in educational institutions can be explained in the discussion of this study
Conflict Management and Performance of Health Care Professionals in Teaching Hospitals in Rivers State
Authors: Adim Chidiebere , Odili Chukwuemeka , Aigboje Patrick 
Published: July 2023

Purpose: The purpose of this study is to conflict management and performance of health care professionals in Teaching Hospitals in Rivers State.
Methodology: The study used a cross sectional research design involving medical doctors, nurses, medical laboratory scientists and pharmacists in University of Port Harcourt Teaching Hospital and Rivers State University Teaching Hospital. A Multi-stage sampling was employed to select 165 healthcare professionals from both hospitals. The reliability of the instrument was achieved by the use of the Cronbach Alpha coefficient with all the items scoring above 0.70. The hypotheses were tested using the Spearman’s Rank Order Correlation Coefficient with the aid of Statistical Package for Social Sciences version 23.0. The tests were carried out at a 95% confidence interval and a 0.05 level of significance.
Findings: Results from analysis of data revealed that conflict management significantly predict performance of health care professionals in Teaching Hospitals in Rivers State.
Recommendation: The study recommends that health care professionals’ collaboration should be adopted in Teaching Hospitals. This includes sharing of patient information, joint participation in the care and in the decision-making process, and degree of cooperation, must be carefully scrutinized and implemented with the added input of nurses, physicians and other paramedics.
Um jogo didático para o ensino das Leis de Newton
Authors: Bonagúrio de Oliveira Jéssica , Santiago de Oliveira Cleidson , Baptestini Elizabeth 
Published: July 2023

Este trabalho descreve como a utilização de um jogo didático contribui para motivar os estudantes nas aulas de Física no Ensino Médio. O jogo de tabuleiro chamado “Corrida Espacial” explora de forma lúdica as Leis de Newton e suas aplicações. O jogo foi aplicado aos estudantes do 1° Ano do Ensino Médio Integrado aoTécnico em Química do IFSP –Capivari, que, após participarem da atividade, responderam um questionário contemplando vários aspectos do jogo. Os resultados obtidos mostram que os estudantes se sentiram motivados e envolvidos no processo de aprendizagem.

Abstract
This paper describes how the use of a didatic game contribuites to motivate High School stundents in Physics class and ground the importance of new methodologies in the teaching-learning process. The board game called “Space Race” explores Newton’s Laws and their aplications in a playful way. The game was applied to the students from the 1st Year of High School Integrated to the Chemistry Technician at IFSP -Capivari, who after play the game responded to a survey about it. The results show that the students felt motivated and involved in the learning process.

Keywords: Educative games; Learning; Motivation; Physics teaching; Newton’s Laws.
Câmpus Capivari
Authors: Lucca Waldo Luis de 
Published: July 2023

Este texto retraça, pela perspectiva de Waldo Luis de Lucca, diretor do IFSP – Câmpus Capivari (Diretor Geral de 2010 a 2020), o histórico do Câmpus Capivari. A partir de dados pessoais e do arcabouço legal, Lucca apresenta um retrospecto institucional.

Abstract
This article retraces, from the perspective of Waldo Luis de Lucca, school principal of IFSP –Câmpu Capivari (from 2010 to 2020), the history of Capivari Campus. Based on personal data and the legal framework, Waldo presents an institutional background.

Keywords: Capivari Campus; Ten years.
História pública e ensino de história
Authors: Vieira Felipe 
Published: July 2023

O presente texto tem por objetivo apresentar uma breve discussão sobre história pública e suas implicações no ensino de História. A discussão é fundamentada na experiência de um projeto de ensino colocado em prática com alunos do Ensino Médio no IFSP-Câmpus Capivari. Considerando as discussões sobre direitos humanos e educação antirracista, o projeto resultou na produção de curtas-metragens que tinham como problemática central pensar o protagonismo de homens e mulheres negros dentro da narrativa da História.

Abstract
This text aims to present a brief discussion about public history and its implications for the teaching of History. The discussion is based on the experience of a teaching project put into practice with high school students at the IFSP-Campus Capivari. Considering the discussions on human rights and anti-racist education, the project resulted in the production of short films thathad as central problem to think the protagonism of black men and women inside the History’s narrative.
Keywords: Public History, anti-racist education, short films.