bluuebunny/crossref_metadata_2025_split huggingface dataset:
Historical Gulfs, Strategic Bridges: A Pragmatic-Symbolic Analysis of the GSOMIA Between Japan and South Korea
Authors: Bronza Ron , Podoler Guy 
Published: January 2025

Abstract
In 2016, Japan and South Korea signed the General Security of Military Information Agreement (GSOMIA)—the first military agreement between the two countries. The agreement called for the sharing of military intelligence concerning North Korea and its missile and nuclear programs. Despite numerous similarities between Japan and South Korea and their shared concerns, the process of signing the GSOMIA was strewn with controversies and setbacks that continue to haunt it long after the signing. This paper aims to understand the interplay between the pragmatic considerations and the symbolic meanings that affected the dynamics surrounding the GSOMIA. The analysis relies on a theoretical framework we offer by linking constructivism with collective memory theory. This approach, we argue, best explains why historical disputes have become extremely influential in Japan-South Korea relations, particularly during the GSOMIA process. It is also argued that, in such a context, the main advantage of the GSOMIA is its limited scope. The agreement is potentially a trust-building mechanism. By partially setting historical differences aside, both countries can build a stronger foundation for regional stability. This, in turn, can serve as a trust-building exercise within a larger process that could help them find an acceptable solution to their difficult shared past.
Aging effects on control ability: an ERP study using the AX continuous performance task in middle-aged and older adults
Authors: Lu Guo , Leng Tian , Chen Li-Ju , Yu Miao , Guan Tao , Liu Yang , Xu Wenhu , Li Dan , Wang Kuo-Pin 
Published: January 2025

Abstract
The dual mechanisms of control model (DMC model) assumes that aging leads to decline in proactive control and reactive control abilities in various aspects of daily life. However, the methods adopted by previous ERP studies limit their capacity to provide in-depth information on the aging effects of neural processes, such as N2 (reactive control), P3b, and CNV (proactive control). This study aims to clarify the aging effects on proactive control and reactive control by controlling additional (potentially confounding) variables (e.g., physical fitness level) and utilizing N2, CNV, and P3b to specify neural processes in the DMC model. To achieve this goal, a total of sixty-two middle-aged and fifty-nine older adults were recruited. Participants underwent the Senior Fitness Test to measure physical fitness level. Furthermore, participants were required to perform the AX-CPT task while ERPs were recorded. The results were primarily focused on the analysis of N2 in AY trials and the analysis of P3b and CNV in BX trials. Notably, older adults exhibited faster reaction times (RT) than middle-aged adults in AY trials. Conversely, in BX trials, older adults displayed slower RT compared with middle-aged adults. Furthermore, our findings indicated that older adults had higher CNV amplitude at the parietal region in comparison with middle-aged adults in BX trials. We suggest that older adults may tend to engage more attentional resources in avoiding the false alarm in AY trials. In contrast, middle-aged adults may engage in a predictive function that directs attention to a particular response in BX trials.
The effect of childhood interpersonal trauma on social anxiety: Implications for using emotion-focused therapy with young adults
Authors: Lee Wooje , Han Dasom , Hagedorn W. Bryce 
Published: January 2025

Abstract
According to emotion-focused therapy (EFT), childhood interpersonal trauma predicts social anxiety in adulthood through internalized shame. While internalized shame is characterized by the negative self-evaluation of the self, the mechanism linking childhood trauma and internalized shame remains unclear. This study examined whether emotional clarity, as posited by EFT, serves as a mediating factor between childhood interpersonal trauma and subsequent internalized shame. Specifically, it investigated the sequential mediating effect of emotional clarity and internalized shame on the relationship between childhood interpersonal trauma and social anxiety among Korean college students. A group of 202 undergraduate students in South Korea participated in the study by completing self-report questionnaires on key variables. The research model was evaluated using structural equation modeling. The results showed that the model fit was acceptable, explaining 46% of the variance for social anxiety. The indirect effect of childhood interpersonal trauma on social anxiety via internalized shame was significant at the p < .05 level. The indirect effect of childhood interpersonal trauma on social anxiety through emotional clarity and internalized shame was also significant at the p < .05 level. The findings support the applicability of an EFT model for working with young adults with social anxiety. Childhood interpersonal trauma can foster a shame-ridden/defective-self scheme, leading to anxiety in social situations where perceived flaws might be ridiculed. Improving one’s emotional clarity is likely to alleviate internalized shame, consequentially reducing social anxiety.
Anaerobic Co-Digestion of Food Waste and Groundnut Shells: Synergistic Impact Assessment and Kinetic Modeling
Authors: Olatunji K. O. , Mootswi K. D. , Olatunji O. O. , Zwane M. I. , van Rensburg N. J. , Madyira D. M. 
Published: January 2025

Abstract
Consumers’ consumption patterns and the global population’s livelihood have led to an increase in waste streams. Food waste (FW), agricultural residues, and livestock wastes are some of the major waste streams that require effective and economical management. This study investigated the biomethane potential, biodegradability, and synergistic effect index of FW and groundnut shells (GS) co-digestion at mesophilic temperature. FW and GS were co-digested at different mixing ratios. In addition, three kinetic models were evaluated and compared for mono and co-digestion. It was discovered that anaerobic co-digestion of FW and GS improved the biomethane released compared to mono-digestion, and the optimum biomethane yield was noticed at 50: 50 with a 32.28% increase. The C/N ratio and biodegradability of FW were enhanced by adding GS as a co-substrate. Co-digestion of the substrates showed a good synergistic influence and reduced the retention time. Pretreatment of GS may be needed to increase the efficiency of the process. Cumulative biomethane yield was fitted with Gompertz, modified Gompertz, and Schnute models, and all the models fit well for both mono and co-digestion of FW and GS. The model production rate is 23.10, 27.78, 23.49, 18.53, and 16.45 mld− 1, and the coefficient of correlation (R2) ranges between 0.97 and 0.99. Anaerobic co-digestion of FW and GS was found to be a suitable combination for improved biomethane production, and kinetic models were considered to have the capacity to describe the anaerobic digestion of FW and GS.

Graphical Abstract
Robust peptide/RNA complexes prepared with microfluidic mixing for pulmonary delivery by nebulisation
Authors: Ma Cheng , Chow Michael Y. T. , Zhang Chengyang , Goldbaum Paulina , Hsieh Jamie Chien-Ming , Lam Jenny K. W. 
Published: January 2025

Abstract
Small interfering RNA (siRNA) and messenger RNA (mRNA) have drawn considerable attention in recent years due to their ability to modulate the expression of specific disease-related proteins. However, it is difficult to find safe, robust, and effective RNA delivery systems suitable for pulmonary delivery to treat lung diseases. In this study, two cationic peptides, namely LAH4-L1 and PEG12KL4, were employed as non-viral vectors for siRNA and mRNA delivery. Four formulations (i.e. LAH4-L1/siRNA; PEG12KL4/siRNA; LAH4-L1/mRNA and PEG12KL4/mRNA) were investigated. Microfluidic mixing method was utilised to fabricate RNA complexes in a controllable and reproducible manner. Upon optimisation of the microfluidic mixing protocol, a vibrating mesh nebuliser was employed to aerosolise the RNA complexes, and their transfection efficiency was evaluated on A549 and BEAS-2B cells. Following nebulisation, inhalable mist was generated for all RNA formulations with mass median aerodynamic diameter below 5 μm. Although the hydrodynamic particle sizes of the RNA complexes were significantly reduced to around 100 nm after nebulisation regardless of the original size of the complexes prior to nebulisation, the RNA binding efficiency and the in vitro RNA transfection ability of all the peptide formulations were successfully preserved with no significant differences compared to the same system before nebulisation. The current result indicates that both LAH4-L1 and PEG12KL4 hold significant potential for future clinical application for pulmonary siRNA and mRNA delivery through nebulisation.

Graphical Abstract
Low compression smart clothing for respiratory rate monitoring using a bending angle sensor based on double-layer capacitance
Authors: Kobayashi Tatsuya , Goto Daisuke , Sakaue Yusuke , Okada Shima , Shiozawa Naruhiro 
Published: January 2025

Abstract
In chronic respiratory diseases, continuous self-monitoring of vital signs such as respiratory rate aids in the early detection of exacerbations. In recent years, the development of smart clothing, such as garments equipped with sensors to measure respiratory rate, has been a focus of research. However, the usability and adoption of smart clothing are often compromised owing to the discomfort caused by compression pressure during wear. This study developed smart clothing designed to measure respiratory rate using a low compression pressure. This was achieved by integrating a bending angle sensor, based on double-layer capacitance, into the rib cage and abdomen areas. The accuracy of the respiratory rate measurement was evaluated in 20 healthy male subjects without respiratory diseases. Breathing was measured while the subjects wore the smart clothing and performed breathing exercises in sitting, supine, and lateral postures, following a metronome set between 12 and 30 bpm. To assess accuracy, the respiratory rate measured by the smart clothing was compared with that measured by a spirometer. The recorded compression pressure was 0.77 ± 0.21 kPa, with no subjects reporting discomfort. Correlation coefficients for respiratory rate in the different postures ranged within 0.97–0.99. The mean difference between the smart clothing and spirometer measurements was less than 0.1 bpm. The low mean difference indicated that the proposed low compression pressure wearable respiration sensor, employing a bending angle sensor based on double-layer capacitance, could measure respiratory rate accurately without causing discomfort and within an acceptable error range.
Near‐Automated Estimate of City Nitrogen Oxides Emissions Applied to South and Southeast Asia
Authors: Lu Gongda , Marais Eloise A. , Vohra Karn , Horner Rebekah P. , Zhang Dandan , Martin Randall V. , Guttikunda Sarath 
Published: January 2025

AbstractCities in South and Southeast Asia are developing rapidly without routine, up‐to‐date knowledge of air pollutant precursor emissions. This data deficit can potentially be addressed for nitrogen oxides (NOx) by deriving city NOx emissions from satellite observations of nitrogen dioxide (NO2) sampled under windy conditions. NO2 plumes of isolated cities are aligned along a consistent wind‐rotated direction and a best‐fit Gaussian is applied to estimate emissions. This approach currently relies on non‐standardized choice of upwind, downwind, and across‐wind distances from the city center, resulting in fits that often fail or yield non‐physical parameters. Here, we propose an automated approach that defines many combinations of distances yielding 54 distinct sampling boxes that we test with TROPOspheric Monitoring Instrument (TROPOMI) NO2 observations over 19 isolated cities in South and Southeast Asia. Our approach is efficient, uses open‐source software, is adaptable to many cities, standardizes and eliminates sensitivity to sampling box choice, increases success of deriving emissions from 40% to 60% with one sampling box to 100% (all 19 cities) with 54, and yields emissions consistent with the current manual approach. We estimate that the annual emissions range from 15 ± 5 mol s−1 for Bangalore (India) to 125 ± 41 mol s−1 for Dhaka (Bangladesh). With enhanced success of deriving top‐down emissions, we find support from comparison to past studies and inventory estimates that top‐down emissions may be biased, as the method does not adequately account for spatial and seasonal variability in NOx photochemistry. Further methodological development is needed for enhanced accuracy and use to derive sub‐annual emissions.
Optimising CPAP and oxygen levels to support spontaneous breathing in preterm rabbits
Authors: Cannata Ebony R. , Crossley Kelly J. , McGillick Erin V. , Wallace Megan J. , Croughan Michelle K. , Jurkschat Dominic , Cramer Sophie J. E. , te Pas Arjan B. , Hooper Stuart B. , Kitchen Marcus J. 
Published: January 2025

Abstract

Background
Very preterm infants often require respiratory support after birth with current recommendations suggesting the use of continuous positive airway pressure (CPAP) of 4–8 cmH2O and an initial fraction of inspired oxygen (FiO2) of 0.21–0.3. We have examined the interaction of high and low CPAP and FiO2 levels on breathing rates and lung aeration in preterm rabbits.

Methods
Prematurely delivered rabbits (29/32 days gestation) received CPAP of either 5cmH2O (5CPAP; n = 12) or 15 cmH2O (15CPAP; n = 14), and a FiO2 of either 0.3 (5CPAP/0.3, n = 6 or 15CPAP/0.3, n = 7) or 0.6 (5CPAP/0.6, n = 6 or 15CPAP/0.6, n = 7). Breathing rates, lung aeration (functional residual capacity; FRC), lung bulging and air accumulation in the stomach were measured using phase-contrast X-ray imaging.

Results
Kittens receiving 0.6 FiO2 had higher breathing rates (5CPAP/0.6: 32.6±6.4 breaths/min; p = 0.0064 and 15CPAP/0.6: 36.9±3.5breaths/min; p = 0.0010) than 5CPAP/0.3 kittens (11.8±4.1breaths/min). Kittens receiving 15CPAP/0.6 tended to have higher FRC volumes (34.9±4 mL/kg) than kittens receiving 5 cmH2O CPAP (5CPAP/0.3: 13.1±6mL/kg; p = 0.0675 and 5CPAP/0.6: 13.5±6 mL/kg; p = 0.1720) and 15CPAP/0.3 (22.5 ± 6.6 mL/kg; p = 0.4245). Lung bulging and air accumulation in the stomach were not different between groups.

Conclusion
Preterm rabbits supported with both 15 cmH2O CPAP and 0.6 FiO2 increased spontaneous breathing rates and lung aeration without increasing the risk of air in the stomach or lung bulging.

Impact

While current guidelines recommend the use of low CPAP (4–8 cmH2O) and low FiO2 levels (0.21–0.3 FiO2) to support preterm infants at birth, the optimum levels are unknown.

This study has shown that 15 cmH2O of CPAP and FiO2 of 0.6 improved lung aeration and breathing in preterm rabbits, compared with a CPAP of 4 cmH2O and FiO2 of 0.3.

These results add to the evidence indicating that initial high CPAP and high FiO2 levels, followed by titration of both, enhance respiratory support for preterm newborns.
piR-26441 inhibits mitochondrial oxidative phosphorylation and tumorigenesis in ovarian cancer through m6A modification by interacting with YTHDC1
Authors: Yuan Jing , Xie Bu-Min , Ji Yu-Meng , Bao Hai-Juan , Wang Jie-Lin , Cheng Jia-Chen , Huang Xiang-Chun , Zhao Yang , Chen Shuo 
Published: January 2025

Abstract
Ovarian cancer (OC) is a heterogeneous cancer. In contrast to other tumor cells, which rely primarily on aerobic glycolysis (Warburg effect) as their energy source, oxidative phosphorylation (OXPHOS) is also one of its major metabolic modes. Piwi-interacting RNAs (piRNAs) play a regulatory function in various biological processes in tumor cells. However, the role and mechanisms of piRNAs in OC and mitochondrial OXPHOS remain to be elucidated. Here, we found that piR-26441 was aberrantly downregulated in OC, and its overexpression suppressed the malignant features of OC cells and tumor growth in a xenograft model. Moreover, overexpression of piR-26441 significantly reduced mitochondrial OXPHOS levels in OC cells. Furthermore, piR-26441 directly binds to and upregulates the expression of YTHDC1 in OC cells. piR-26441 also increased m6A levels, thereby interacting with YTHDC1 to destabilize the mRNA of TSFM. The resultant TSFM loss reduced mitochondrial complex I activity and mitochondrial OXPHOS, leading to mitochondrial dysfunction in OC cells, increased reactive oxygen species levels, and thus, DNA damage and apoptosis in OC cells, thereby inhibiting OC progression. Additionally, ago-piR-26441 suppressed tumor growth and mitochondrial metabolism in the patient-derived organoid model. Altogether, piR-26441 could inhibit OC cell growth via the YTHDC1/TSFM signaling axis, underscoring its significant importance in the context of OC, as well as offering potential as a therapeutic target.
CDK14 regulates the development and repair of lung
Authors: Chen Jian-wei , Wang Yu-xiang , Gao Rong-rong , Ma Lan-yue , Zhong Jing , Yang Jia-xin , Deng Zhao-hua , Li Yu-yan , Li Xiao-ling , Shu Ya-hai , Guo Wen-jing , Zhou Zi-yuan , Tian Xiao Yu , Ma Jinjin , Liu Yang , Chen Qi 
Published: January 2025

Abstract
Cyclin-dependent kinases (CDK) 14 regulates cell cycle, tumor expansion by influencing the downstream targets of the canonical Wnt signaling pathway. However, the function of CDK14 during organ development and regeneration has not been investigated in genetically-modified animals. Here, we found that genetic ablation of Cdk14 influenced pulmonary vascular endothelial cells and alveolar epithelial cells during mice embryonic development as well as repair of lung after bleomycin or lipopolysaccharide induced injury. Genetic knockout of Cdk14 and the CDK14 covalent inhibitor FMF-04-159-2 resulted in reduction of pulmonary vessel covered area and epithelial cell number, exhibiting increased mortality and more severe lung damage after injury. Mechanistically, Cdk14 ablation inhibited the proliferation of epithelial and vascular endothelial cells, inducing cell cycle arrest at the G2/M phase. Through RNA-seq analysis of both endothelial and epithelial cells, we found that knockdown of Cdk14 controls the expression of signal transducers and activator of transcription 1 (STAT1) as well as associated genes in interferon signaling. Disruption of Cdk14 interferes with IFN-γ induced lung repair in vivo, suggesting potential crosstalk of CDK14 signaling and IFN-γ pathway. Our work highlights the importance of Cdk14 in lung development and regenerative repair through an uncharacterized CDK14- IFN-γ signaling axis.
Numerous rRNA molecules form the apicomplexan mitoribosome via repurposed protein and RNA elements
Authors: Shikha Shikha , Tobiasson Victor , Ferreira Silva Mariana , Ovciarikova Jana , Beraldi Dario , Mühleip Alexander , Sheiner Lilach 
Published: January 2025

Abstract
Mitochondrial ribosomes (mitoribosomes) are essential, and their function of synthesising mitochondrial proteins is universal. The core of almost all mitoribosomes is formed from a small number of long and self-folding rRNA molecules. In contrast, the mitoribosome of the apicomplexan parasite Toxoplasma gondii assembles from over 50 extremely short rRNA molecules. Here, we use cryo-EM to discover the features that enable this unusual mitoribosome to perform its function. We reveal that poly-A tails added to rRNA molecules are integrated into the ribosome, and we demonstrate their essentiality for mitoribosome formation and for parasite survival. This is a distinct function for poly-A tails, which are otherwise known primarily as stabilisers of messenger RNAs. Furthermore, while ribosomes typically consist of unique rRNA sequences, here nine sequences are used twice, each copy integrated in a different mitoribosome domain, revealing one of the mechanisms enabling the extreme mitochondrial genome reduction characteristic to Apicomplexa and to a large group of related microbial eukaryotes. Finally, several transcription factor-like proteins are repurposed to compensate for reduced or lost critical ribosomal domains, including members of the ApiAP2 family thus far considered to be DNA-binding transcription factors.
Structural studies of the IFNλ4 receptor complex using cryoEM enabled by protein engineering
Authors: Grubbe William S. , Zhang Bixia , Kauffman Aileen , Byléhn Fabian , Padoł Kasia , Jung Hae-Gwang , Park Seung Bum , Priest Jessica M. , Özkan Engin , de Pablo Juan J. , Liang T. Jake , Zhao Minglei , Mendoza Juan L. 
Published: January 2025

Abstract
IFNλ4 has posed a conundrum in human immunology since its discovery in 2013, with its expression linked to complications with viral clearance. While genetic and cellular studies revealed the detrimental effects of IFNλ4 expression, extensive structural and functional characterization has been limited by the inability to express and purify the protein, complicating explanations of its paradoxical behavior. In this work, we report a method for robust production of IFNλ4. We then use yeast surface display to affinity-mature IL10Rβ and solve the 72 kilodalton structures of IFNλ4 (3.26 Å) and IFNλ3 (3.00 Å) in complex with their receptors IFNλR1 and IL10Rβ using cryogenic electron microscopy. Comparison of the structures highlights differences in receptor engagement and reveals a distinct 12-degree rotation in overall receptor geometry, providing a potential mechanistic explanation for differences in cell signaling, downstream gene induction, and antiviral activities. Further, we perform a structural analysis using molecular modeling and simulation to identify a unique region of IFNλ4 that, when replaced, enables secretion of the protein from cells. These findings provide a structural and functional understanding of the IFNλ4 protein and enable future comprehensive studies towards correcting IFNλ4 dysfunction in large populations of affected patients.
The first geospatial dataset of irrigated fields (2020–2024) in Vojvodina (Serbia)
Authors: Radulović Mirjana , Marković Miljana , Brdar Sanja , Athanasiadis Ioannis , Mimić Gordan 
Published: January 2025

Abstract
Irrigation is a cornerstone of global food security, enabling sustainable agricultural production and helping to ensure that food is available for people around the world, now and in the future. Mapping irrigated fields provides valuable information for sustainable water management, agricultural development, and environmental conservation efforts. However, the collection of high-quality training data, which is necessary for accurate irrigation mapping remains costly and labour-intensive. To address this, we created a georeferenced regional dataset consisting of location, crop type, and occurrence of the irrigation equipment which are essential information for mapping irrigated fields. Four main irrigated crops were considered: maize, soybean, sugar beet, and wheat. The dataset, consisting of a total of 1256 parcels, is created for Vojvodina, the main agricultural area in Serbia, spanning the period of five years (2020–2024). This study’s goal is to give accessibility to our dataset which further can be explored and used for building or fine-tuning machine learning and deep learning models for the automatic detection of irrigated fields using satellite imagery.