bluuebunny/crossref_metadata_2025_split huggingface dataset:
A comparative evaluation of CRISPR-Cas9 allele editing systems inCandida auris: challenging research in a challenging bug
Authors: Sofras Dimitrios , Carolus Hans , Subotić Ana , Romero Celia Lobo , Ennis Craig L. , Hernday Aaron D. , Nobile Clarissa J. , Rybak Jeffrey M. , Van Dijck Patrick 
Published: January 2025

AbstractCandida aurisis an emergent fungal pathogen of significant interest for molecular research because of its unique nosocomial persistence, high stress tolerance and common multidrug resistance. To investigate the molecular mechanisms of these or other phenotypes, a handful of CRISPR-Cas9 based allele editing tools have been optimized forC. auris. Nonetheless, allele editing in this species remains a significant challenge, and different systems have different advantages and disadvantages. In this work, we compare four systems to introduce the genetic elements necessary for the production of Cas9 and the guide RNA molecule in the genome ofC. auris,replacing theENO1,LEU2andHIS1loci respectively, while the fourth system makes use of an episomal plasmid. We observed that the editing efficiency of all four systems was significantly different and strain dependent. Alarmingly, we did not detect correct integration of linear CRISPR cassette constructs in integration-based systems, in over 4,900 screened transformants. Still, all transformants, whether correctly edited or not, grew on selective nourseothricin media, suggesting common random ectopic integration of the CRISPR cassette. Although the plasmid-based system showed a low transformation success compared to the other systems, it has the highest editing efficiency with 41.9% correct transformants on average. In an attempt to improve editing efficiencies of integration-based systems by silencing the non-homologous end joining (NHEJ) DNA repair pathway, we deleted two main NHEJ factors,KU70andLIG4. However, no improved editing or targeting efficiencies were detected inku7011, lig411,orku7011/lig411backgrounds. Our research highlights important challenges in precise genome editing ofC. aurisand sheds light on the advantages and limitations of several methods with the aim to guide scientists in selecting the most appropriate tool for molecular work in this enigmatic fungal pathogen.Author summaryCandida aurisis a rapidly emerging fungal pathogen that poses serious challenges to global healthcare. Understanding the genetic mechanisms that underlie its nosocomial persistence, virulence, multidrug resistance and other traits is essential for developing new treatments and preventing the spread and burden ofC. aurisinfections. However, precise genetic manipulation inC. aurishas proven difficult due to inefficient genome editing tools. This study compares four different CRISPR-based allele editing systems inC. auris, identifying their strengths and limitations. The findings provide crucial insights into selecting the best tools for genetic research inC. auris, guiding future efforts to combat this formidable pathogen.
A switch in the mode of tissue growth extends the growth phase ofDrosophilawing primordia during early pupal development
Authors: El Marzkioui Khaoula , Gaugué Isabelle , De Giorgio Ettore , Léopold Pierre , Boulan Laura 
Published: January 2025

ABSTRACTUnderstanding how final organ size is established during development raises two key questions: how organs increase their mass and how they stop growing upon reaching an appropriate size. While organ growth is driven by conserved signaling pathways, the mechanisms underlying growth arrest remain elusive. Studies onDrosophilaimaginal wing discs have provided a model whereby final organ size coincides with an arrest of cell proliferation at the end of the larval phase. Here, through 3D reconstruction and volume measurements, we show that wing discs grow continuously throughout the larva-to-pupa (L/P) transition and proceed to growth arrest later during the pupal period. This supplemental growth phase involves a switch at the L/P transition that uncouples proliferation from tissue growth, with an important contribution from Insulin/IGF signaling. These findings challenge the existing model of imaginal wing development and open new avenues for our understanding of growth arrest and organ size determination.
Clinical outcomes of Lassa fever in West Africa: A systematic review and meta-analysis
Authors: Okwuraiwe Azuka Patrick , Onyeaghala Chizaram Anselm , Ozoude Obiageli Theresa , Suleiman Muritala Odidi , Abdu-Aguye Samirah Nndwan , Ezekwelu Nkolika Jacinta , Oyeniyi Tolulope Amos , Jegede Ayodapo Oluwadare , Egwudo Adaeze Elfrida , Okeke Oluchukwu Perpetua , Abodunrin Olunuke Rebecca , Akinsolu Folahanmi Tomiwa , Sobande Olajide Odunayo 
Published: January 2025

AbstractIntroductionLassa fever is an acute viral haemorrhagic fever that poses a substantial public health security threat in West Africa. Due to its non-specific clinical manifestations and the absence of a reliable point-of-care test, diagnosis could be delayed, leading to severe complications and mortality during epidemics. This systematic review aimed to determine the clinical outcomes of LF in West Africa.MethodsA systematic review and meta-analyses were performed by conducting an extensive online search using PubMed, Web of Science, Scopus, CINAHL, and Google Scholar (PROSPERO protocol CRD42024587426). Only articles written in English were included in publications from 2014 to 2024. The analysis followed PRISMA guidelines. The mortality rate of LF was pooled using a random effects model.ResultsWe included 19 studies that contained data from 4177 patients hospitalized with LF of any age. Most of the studies were of retrospective cohort (16/19; 84.2%) study design and were predominantly conducted in Nigeria (16/19; 84.2%). The mortality rate was highest in a Sierra Leonean study (63.0%), whereas group-based analysis among the Nigerian studies identified Owo as having the highest mortality rate of 13% (95% CI: 06-23; I2=98%). The pooled LF mortality rate was 19% (95% confidence interval [CI]:10-32). The most common complications of LF are acute kidney injury (AKI) at a pooled proportion of 19% (95% CI; 13-26; I2=89%)), followed by abnormal bleeding at a pooled proportion of 17% (95% CI; 9-30; I2=98%), and CNS manifestations at a pooled proportion of 15% (95% CI; 6-32; I2=98%)).ConclusionWith one out of every five hospitalized Lassa Fever patients likely to die in West Africa, accelerating the development of rapid diagnostic tests, licensed vaccines, and novel therapeutics is crucial. Strengthening community engagement, risk communication, and healthcare worker training will enhance early diagnosis and effective case management thereby reducing mortality and severe complications.
Assessing mosquito dynamics and dengue transmission in Foz do Iguaçu, Brazil through an enhanced temperature-dependent mathematical model
Authors: Rauh C. S. , Araujo E. C. , Ganem F. , Lana R. M. , Leandro A. S. , Martins C. A. , Coelho F. C. , Codeço C. T. , Bastos L. S. , Pinho S. T. R. 
Published: January 2025

AbstractDengue fever is a public health concern that demands efforts to mitigate its impact. We aim to investigate the influence of key parameters temperature-dependent on dengue transmission dynamics in Foz do Iguaçu, a triple border municipality in south Brazil, applying a mathematical model composed by systems of ordinary differential equations. Adjusted model simulation is consistent with the observed data. The effective reproduction number was calculated for detecting changes in dengue transmission over time and to timely detect the beginning of epidemics. Additionally, we explore the potential effects of climate variability on dengue dynamics. Our findings show the importance of vector population dynamics, climate and incidence, contributing to a deeper understanding of dengue transmission dynamics in Foz do Iguaçu and providing a foundation for optimizing intervention strategies, also, in other cities, enhancing our ability to predict and manage dengue outbreaks and supporting public health efforts to control measures.
Benchmarking the best practices of Lean Six Sigma in aerospace industry in India: a longitudinal multiple case analysis and roadmap for deployment
Authors: Bhat Shreeranga , Gijo E V , Bhat Vinayambika S 
Published: January 2025

PurposeThis article intends to comprehend the Lean Six Sigma (LSS) approach adopted in the aerospace industry in India for process improvement. This research has the objective of determining LSS deliverables such as Voice of Customer (VOC), Key Performance Indicators (KPIs), Critical-to-Quality (CTQ), project approach, deployment strategies and tools and techniques used to execute the project.Design/methodology/approachThe study adopted an exploratory research methodology and a multiple case study analysis to draw robust inferences. The research is carried out in the Indian aerospace industry and analyses five case studies. The case studies were collated from the company via a case study protocol with pre-defined criteria. Also, a semi-structured interview is conducted with the stakeholders of each case study to determine the deployment strategies followed during the respective projects.FindingsIt is reconfirmed that LSS is crucial in the aerospace industry, particularly in engine and gear shops, to reduce rework and rejections. Also, it was found that cost and time savings are essential KPIs. Some LSS projects require multiple CTQs for process improvement in aero industries. The DMAIC approach is used for project execution, with the Design of Experiment (DOE) being an essential tool. Top management engagement, effective HRM practices, customer focus, cross-functional collaboration and clear roles are essential for successful LSS projects. Eventually, a road map was developed based on the analysis of multiple case studies.Research limitations/implicationsThe study is focused on the aerospace industry in India, which may limit the generalizability of the findings to other industries or regions. The small sample size and reliance on qualitative data through semi-structured interviews may introduce subjectivity. Additionally, the long-term effects of LSS implementation, particularly in the context of evolving technologies, were not fully explored.Practical implicationsThis study provides actionable insights for aerospace companies and related organisations to enhance quality and operational performance. The developed roadmap offers a practical guide for LSS deployment, helping organisations improve efficiency and competitiveness, especially in an era of economic slowdown and high competition.Originality/valueThe study reveals similarities and differences in LSS deliverables in Indian aerospace industries, creating a roadmap and tool matrix for project execution and serving as a template for manufacturing industries.
Harnessing innovation and data: how do digital M&As fuel new quality productive forces
Authors: Xu Jinhua , Zhang Jiaye , Li Xiaoxia 
Published: January 2025

PurposeNew quality productive forces (NQPF) are critical for high-quality economic development. As digital mergers and acquisitions (M&As) gain prominence in corporate digital transformation, understanding their impact on NQPF is essential. This study explores whether digital M&As enhance NQPF in firms and identifies key mechanisms that drive this effect.Design/methodology/approachThis study investigates the impact of corporate digital M&As on NQPF using a multi-period difference-in-difference (DID) methodology. Analyzing a sample of Chinese listed firms from 2011 to 2021, the study explores how digital M&As contribute to NQPF, identifying firm innovation and data assets as key mechanisms. It also examines how external factors, such as industrial structure, urban human capital and economic policy uncertainty, moderate the effect of digital M&As on NQPF.FindingsThe study reveals three key findings: (1) Digital M&As significantly enhance corporate NQPF; (2) innovation and data assets serve as key mechanisms through which digital M&As drive NQPF and (3) external factors, including industrial structure, urban human capital and economic policy uncertainty, amplify the positive effects of digital M&As on NQPF.Practical implicationsFirms should leverage digital M&As as a strategic tool for improving NQPF, focusing on innovation and data assets. Policymakers can support this transformation by fostering an environment that enhances the positive impact of digital M&As on economic development.Originality/valueThis paper introduces a novel NQPF index, offering a comprehensive measurement of the concept. It provides new insights into how digital M&As affect NQPF, filling a gap in the literature on digital transformation and offering actionable recommendations for firms and policymakers.
Facebook’s investment in Jio Platform, India: the big deal
Authors: Aggarwal Shalini , Pahuja Anurag , Jha Suchita , Sethi Madhvi 
Published: January 2025

Learning outcomes
After completion of the case study, the students will be able to analyze the overall competitive environment for telecom sector in India with the use of Herfindahl–Hirschman index tool, execute the fundamental analysis of Jio Platforms Limited (JPL) company, understand the concept of net neutrality and its implications for India market, understand the concept of “Zero debt company” and its implication for companies and understand data privacy concerns.

Case overview/synopsis
In early September of 2020, Ashish Aggarwal, a businessman in the northern state of Punjab, India with his usual habit of turning the pages of newspaper on hand and sipping morning chai got excited while reading newspaper with recent investment of Meta Platforms via Facebook buying 9.99% stake in reliance JPL. He explored and saw the potential for small businesses to invest in this and earn money, as a finance-inclined individual Aggarwal thought why not invest and earn from this opportunity. So he started googling and saw all the reviews of analysts on investment site and investors predicted that the deal could be a game changer that would further transform the existing telecom and social media platforms in India. The deal would further open doors for a new market “JioMart” which could be a futuristic design for a “one-stop-shop for e-commerce, social media consumption, instant messaging, and also digital payments”. Mukesh Ambani’s intentions to make the company zero net debt company within next 18 months fascinated him too. All this made Aggarwal interested in investing in JPL on behalf of his company as this investment option was only for business investors. So he called his financial manager, Mr Anish Mahajan for discussing the issue and both started discussing what could be the future of investment, then he told Mahajan to do the complete analysis. Also, Aggarwal was perplexed about the impact that Meta’s investment in Jio would have on consumers, especially in India. How would the telecom regulatory authority in India view it? Aggarwal felt that how the telecommunication industry would plunge into disruption mode in future. What would be the competitors’ stance in India? Would it trigger other players for strategic alliances? Aggarwal was in a dilemma whether he should invest his money in JPL company or not with controversial discussion on net neutrality and data privacy concern?

Complexity academic level
This case study is suited to master degree programs.

Supplementary materials
Teaching notes are available for educators only.

Subject code
CSS 1: Accounting and Finance.
Digital transformation and the spillover of green innovation: evidence from peer firms in heavily polluting industries
Authors: Pei Xudong , Song Juan , Li Na , Cao Borui 
Published: January 2025

PurposeIt is found that previous studies only focus on how digital transformation contributes to individual firms’ green innovation performance while ignoring the important role that it plays in the spillover and diffusion of green innovations among peer firms. Therefore, this study aims to investigate the influence of focal firms’ digital transformation on the spillover of green innovation among peer firms in heavily polluting industries mediated by environmental, social and governance (ESG) performance and agency conflict. Further, this study is also expected to explore the effects of digital transformation’s green innovation spillover.Design/methodology/approachThis study chooses 6,438 A-share heavily polluting listed firms in the stock exchanges based in Shanghai and Shenzhen in China during 2010–2020 as samples and tests the hypothesis with ordinary least squares (OLS) regression. Results prove to be robust to a battery of robustness analyses the authors performed to take care of endogeneity.FindingsThe results show that the focal firm’s digital transformation may trigger their peer firms’ green innovation spillover and prompt them to engage in green innovation activities actively. The mechanism test shows that peer firms’ ESG performance and agency conflict mediate the influence path between digital transformation and peer firms’ green innovation spillover. Finally, among heavily polluting firms with high industry competition and large scale, digital transformation’s green innovation spillover effects are more significant in conventional energy-based source control, end-of-pipe treatment and substantive green innovation.Originality/valueThis study is possible to provide a potential driving mechanism of green innovation spillovers. The findings lay a sound foundation for future research, providing important theoretical support and practical insights for digital transformation to empower heavily polluting industries to achieve green transformation and low-carbon development.
International experience of community support for national park development and its implications
Authors: Kong Shuyi , Xie Mengling , Zhang Wei , Xia Chunfeng , Yi Xie , Solomon Tamirat , Yin Xinan , Liu Haifei , Wang Changhai 
Published: January 2025

PurposeThis article aims to explore the key role of community participation in the protection and development of national parks under the global trend of national park development and provide reference for the construction of China’s national park system by analyzing international successful cases and experiences.Design/methodology/approachThe study on “International Experience of Community Support for National Park Development” integrates multimethods, from data mining reports, journals and policy docs from WB, UNEP, to case analyses. In-depth interviews with policymakers, academics and farmers reveal needs, challenges and best practices. Comparative analysis tailors findings to China’s context, offering recommendations for enhancing community support. This hybrid approach ensures practical insights for China’s application.FindingsThe results of this study underscore the paramount significance of community participation as a cornerstone in advancing sustainable development and safeguarding national parks amidst a growing global awareness of environmental stewardship. Through a thorough examination of international National Parks such as Yellowstone, Maasai Mara, Uluru-Kata Tjuta and Sanjiangyuan, we reveal a blueprint of success that hinges on robust policy support, empowerment of local communities, strategic economic incentives and multifaceted cross-sectoral collaborations. In the context of domestic hurdles, including inadequate legal frameworks, narrow participation avenues and resource scarcities, our analysis outlines actionable recommendations aimed at fortifying policy and legal frameworks, establishing efficient engagement modalities, bolstering community capacity-building initiatives and fostering economic sustainability. This comprehensive approach presents a visionary roadmap for World’s national park system, guiding it towards achieving an optimal equilibrium where ecological integrity and community prosperity coexist harmoniously.Originality/valueThe article underscores the originality in illuminating the pivotal role of community participation in national park protection and development amidst a global shift. By delving into international exemplars like Yellowstone, Maasai Mara, Uluru-Kata Tjuta, Sanjiangyuan and Panda, it uncovers novel insights on policy frameworks, community empowerment, economic incentives and collaborative models. This work contributes to the burgeoning discourse on balancing ecological conservation with socioeconomic development, providing a blueprint for sustainable national park management of all the world.
An examination of the coaching quality checklist using confirmatory factor analysis
Authors: Hardy Jessica K. , Marcoulides Katerina , Grifenhagen Jill , McLeod Ragan H. , Hemmeter Mary Louise 
Published: January 2025

PurposeWe developed and studied an approach to measuring the quality of coaching meetings. Coaching is a professional development approach that has been implemented in education settings for several decades to support teachers and other practitioners in providing effective instruction. As coaching has become more prevalent, it has become clear that the field needs tools to measure coaching quality.Design/methodology/approachThe coaching quality checklist (CQC) is a measure based on the empirical and theoretical literature on coaching. It has 26 items designed to measure three constructs: foundational, supportive and change-oriented coaching skills. In this study, we conducted a confirmatory factor analysis of the CQC.FindingsWe found the one-factor model fit the data well. The hypothesized higher-order three subfactor model fit the data better but not significantly so. Additional research is needed to further validate the CQC using a larger sample and examine different types of validity.Originality/valueThe CQC is a promising tool for measuring coaching quality, which can help ensure that teachers are provided with high-quality professional development to support their use of interventions.
Information adoption of brand messages among Generation Z in trending topics
Authors: Xin Rujing , Lim Yi Jing 
Published: January 2025

PurposeThis study investigated how Generation Z adopted brand messages through trending topics and analysed the central and peripheral routes of information adoption. It explored the roles of informational and emotional supports in influencing information adoption through perceived information usefulness and considered the adoption of brand messages within trending topic discussions.Design/methodology/approachDrawing on the extension information adoption model, this study employed a quantitative approach by utilising covariance-based structural equation modelling to analyse 400 valid web-based questionnaire responses from Sina Microblog users among Generation Z in China.FindingsAdopting brand messages via trending topics, with cues emphasising information quality as the central route and information credibility as the peripheral route, enhances perceived information usefulness. Notably, Generation Z can derive informational and emotional support from trending topic discussions, influencing their perceived information usefulness. This process operates independently of the dual routes of information adoption.Research limitations/implicationsMarketers can leverage influencers and users’ comments to motivate Generation Z to adopt brand messages through trending topics, but this study was limited by the demographic diversity of its sample and the specific types of brands analysed. Incorporating cross-cultural comparisons, group analyses, potential moderating factors and control variables could improve the model’s generalisability.Originality/valueCompared to information adoption models rooted in the organisational information exchange, the originality of this study lies in confirming that, within the context of trending topics, which blend social connections with an anonymous environment, informational and emotional supports can serve as additional cues influencing brand information adoption via perceived information usefulness.