bluuebunny/crossref_metadata_2025_split huggingface dataset:
Acute Toxicity of Copper to Three Species of Pacific Salmon Fry in Water with Low Hardness and Low Dissolved Organic Carbon
Authors: Porter Drew E. , Morris Jeffrey M. , Trifari Michelle P. , Wooller Matthew J. , Westley Peter A. H. , Gorman Kristen B. , Barst Benjamin D. 
Published: July 2023

Abstract
Proposed development of a mine within Alaska's Bristol Bay watershed (USA) has raised concerns about the potential impact of copper (Cu) on Pacific salmon (Oncorhynchus spp.). We conducted 96-h flow-through bioassays using low-hardness and low dissolved organic carbon water to determine the acute lethal toxicity of Cu to sockeye (Oncorhynchus nerka), Chinook (Oncorhynchus tshawytscha), and coho salmon (Oncorhynchus kisutch) fry. We aimed to determine Cu toxicity under field-relevant water quality conditions and to assess three methods of calculating ambient Cu criteria: the biotic ligand model (BLM), a multiple linear regression model endorsed by the US Environmental Protection Agency, and the hardness-based model currently used by the State of Alaska. The criteria generated by all models were below 20% lethal Cu concentrations by factors ranging from 2.2 to 54.3, indicating that all criteria would be protective against mortality. The multiple linear regression-based criteria were the most conservative and were comparable to BLM-based criteria. The median lethal concentrations (LC50s) for sockeye, Chinook, and coho were 35.2, 23.9, and 6.3 µg Cu/L, respectively. We also used the BLM to predict LC50s for each species. Model predictions differed from empirical LC50s by factors of 0.7 for sockeye and Chinook salmon, and 1.1 for coho salmon. These differences fell within the acceptable range of ±2, indicating the model's accuracy. We calculated critical lethal Cu accumulation values for each species to account for differing water chemistry in each bioassay; the present study revealed that coho salmon were most sensitive to Cu, followed by sockeye and Chinook salmon. Our findings underscore the importance of considering site- and species-specific factors when modeling Cu toxicity. The empirical data we present may enhance Cu risk assessments for Pacific salmon. Environ Toxicol Chem 2023;42:2440–2452. © 2023 SETAC
Nuciferine ameliorates high‐fat diet‐induced disorders of glucose and lipid metabolism in obese mice based on the gut–liver axis
Authors: Zhu Xiangyang , Hao Rili , Lv Xiaqing , Zhou Xing , Li Dapeng , Zhang Chen 
Published: July 2023

AbstractHigh‐fat diets (HFDs) can trigger lipid accumulation, insulin resistance, and inflammatory responses in the body, causing glucose and lipid metabolism dysfunction. This study aimed to explore the role of nuciferine on HFD‐induced disorders of glucose and lipid metabolism in obesity mice models, and further to identify the possible underlying mechanisms. In our experiments, nuciferine effectively ameliorated HFD‐induced insulin resistance by IRS/PI3K/AKT pathway, and alleviated HFD‐induced lipid accumulation via AMPK‐mediated regulation of FAS and ATGL expression. Nuciferine also suppressed HFD‐induced intestinal pathological damage, as evidenced by the improvement of intestinal barrier integrity and the increasing in expressions of tight junction proteins of claudin, ZO‐1, and occludin. In addition, 16S rRNA gene sequencing of the fecal microbiota suggested that nuciferine changed the diversity and the composition of gut microbiota in HFD‐fed mice. Nuciferine decreased the ratio of Firmicutes/Bacteroidetes, the relative abundance of LPS‐producing genus Desulfovibrio and the bacteria which involved in lipid metabolism. In summary, nuciferine ameliorated the disorders of glucose and lipid metabolism in obese mice via balancing the gut–liver axis.
A method to classify coal pore system by using cumulative amplitude ratio and its dynamic variation
Authors: Zou Mingjun , Cai Ningbo , Wang Keying , Ding Zibin , Yao Linlin 
Published: July 2023

AbstractAs coal pore development is decisive for choosing the engineering site and predicting the CO2 storage capacity, this paper provides a new method to define the double T2 cutoff values by using cumulative amplitude ratio measured by nuclear magnetic resonance measurements, classifies the coal pore systems, and analyzes the influences on cumulative amplitude ratio. The following cognitions are achieved. The minimum ratio always varies narrowly and ranges from 0.9 to 1.1, which is quite stable and approximately equals to 1. Ranges of maximum and average ratios are 1.2–3.5 and 1.1–1.8, respectively. T2c1 represents the dividing point of diffusion pore and permeation pore, and its average value is about 4.1 ms. T2c2 represents the dividing point of permeation pore and cleat, with an average value of about 81.9 ms. The volumetric proportions of diffusion pore range from 1.5% to 76.2%, with an average value of 34.6%; the volumetric proportions of permeation pore are from 14.9% to 98.5%, with an average of 46.8%; while the volumetric proportions of cleat are between 8.4% and 57.5%, with an average of 26.6%. According to the different influencing degrees on maximum and average ratios, three types of parameters can be divided. The first type is strong correlation parameters and includes permeability, volumetric percentage of cleat, and relative volumetric percentage of cleat. The second type is medium correlation parameters, such as volumetric percentage of diffusion pore. The third type is weak correlation parameters, including T2 cutoff values, porosity, and maximum vitrinite reflectance. © 2023 Society of Chemical Industry and John Wiley & Sons, Ltd.
Prophylactic low‐molecular‐weight heparin in women with thrombophilia undergoing in vitro fertilization
Authors: Lambert José Roberto , Ueno Joji , Fernandes César Eduardo , Pompei Luciano Melo 
Published: July 2023

AbstractObjectiveTo verify whether low‐molecular‐weight heparin (LMWH) could increase pregnancy rates and/or decrease abortion rates in women with thrombophilia undergoing assisted reproduction cycles.MethodsCross‐sectional study with patients undergoing in vitro fertilization (IVF) (N = 104). Women without thrombophilia (control group, n = 20), women with thrombophilia who did not receive LMWH (untreated group, n = 30), and women with thrombophilia, treated with daily enoxaparin from the day of embryo transfer until week 36 of gestation (treated group, n = 54). All women underwent controlled ovarian hyperstimulation. IVF was performed by intracytoplasmic sperm injection, and embryos were transferred on day 3. Pregnancy was detected by β‐human chorionic gonadotropin (biochemical pregnancy) and fetal heartbeat at week 5 to 6. Ongoing pregnancy was determined by ultrasound on week 12.ResultsPatients in the untreated thrombophilia group presented with significantly lower ongoing pregnancy rates and live birth rates and significantly higher early pregnancy loss and abortion rates when compared with the control and the treated thrombophilia groups.ConclusionsIn women with diagnosed coagulation disorders, use of LMWH is important to avoid miscarriages.
Elastic modulus of hydrogel regulates osteogenic differentiation via liquid–liquid phase separation of YAP
Authors: Tan Kuang , Yang Qiaolin , Han Yineng , Zhuang Ziyao , Zhao Yi , Guo KunYao , Tan Anqi , Zheng Yunfei , Li Weiran 
Published: July 2023

AbstractCraniofacial bone defects induced by congenital malformations, trauma, or diseases frequently challenge the orthodontic or restorative treatment. Stem cell‐based bone regenerative approaches emerged as a promising method to resolve bone defects. Microenvironment physical cues, such as the matrix elastic modulus or matrix topography, regulate stem cell differentiation via multiple genes. We constructed gelatin methacryloyl (GelMA), a well‐known scaffold, to investigate the impact of elastic modulus on osteogenic differentiation in a three‐dimensional environment. Confocal microscope was used to observe and assess the condensates fission and fusion. New bone formation was evaluated by micro‐computed tomography at 6 weeks in calvarial defect rat. We found that the light curing increased elastic modulus of GelMA, and the pore size of GelMA decreased. The expression of osteogenic markers was inhibited in hBMSCs cultured in the low‐elastic‐modulus GelMA. In contrast, the expression of YAP, TAZ and TEAD was increased in the hBMSCs in the low‐elastic‐modulus GelMA. Furthermore, YAP assembled via liquid–liquid phase separation (LLPS) into condensates that were sensitive to 1′6‐hexanediol. YAP recruit TAZ and TEAD4, but not RUNX2 into the condensates. In vivo, we also found that hBMSCs in high‐elastic‐modulus GelMA was more apt to form new bone. This study provides new insight into the mechanism of osteogenic differentiation. Reagents that can regulate the elastic modulus of substrate or LLPS may be applied to promote bone regeneration.
Electrochemical evaluation of magnetic reduced graphene oxide nanosheet‐modified glassy carbon electrode on dopamine electrochemical sensor for Parkinson's diagnostic application
Authors: Hardiansyah Andri , Saputra Gardin Muhammad Andika , Hikmat Hikmat , Kusfarida Yuniar Elfira , Septiani Ni Luh Wulan , Randy Ahmad , Hermawan Angga , Yuliarto Brian , Liu Ting‐Yu , Kida Tetsuya 
Published: July 2023

AbstractBackgroundDopamine is an important catecholamine neurotransmitter that plays a critical function as a chemical messenger that aids in the signal transmission in the brain and other important places. Dopamine is known to play an important role in several neurological diseases such as schizophrenia and Parkinson's disease in which a key factor in the development of Parkinson's disease is abnormally lower levels of dopamine compared to normal levels. However, these methods are expensive, requires manual labor and resources, and demands large number of reagents and chemicals. Therefore, it is essential to develop dopamine detection methods that are less costly, more stable, and easier to perform.ObjectiveTo develop a magnetic reduced graphene oxide (MRGO) nanosheet‐modified glassy carbon electrode (GCE) for the electrochemical detection of dopamine.MethodsMagnetic reduced graphene oxide (MRGO) nanosheet were prepared by the in situ coprecipitation of Fe3+ and Fe2+ in the GO solution. To prepare the MRGO nanosheet ink, 38 mg of MRGO nanosheet was mixed with 2 mg of acetylene black and 80 μL of Nafion solution (5%). The mixture was then mixed with 210 μL of deionized water and 210 μL of 2‐propanol and subjected to ultrasonication for 120 min using a sonicator to obtain a homogenous MRGO nanosheet ink. Furthermore, 3 μL of MRGO ink was dripped onto the surface of the GCE.ResultsMRGO consists of graphene layered tethered with the spherical Fe3O4. The oxygen‐based functional groups on the surface of MRGO could act as chemically active sites for the attachment of dopamine. The MRGO nanosheet has been proven to be an effective GCE modifier with a remarkable electroactive surface area of approximately 0.0104 cm2, demonstrating stable and sensitive electrochemical performance towards dopamine.ConclusionsThe MRGO nanosheets‐modified GCE presented a great potential as an electrocatalyst for dopamine detection, especially in the presence of interfering compounds like uric acid. By effectively discriminating dopamine and uric acid, MRGO nanosheets‐modified GCE have demonstrated a sensitive, stable, and specific electrochemical performance. This remarkable result underlines the immense potential of MRGO nanosheets‐modified GCE for real‐world applications, particularly in the medical field, where the ability to detect dopamine precisely and accurately is critical.
Need for additional training to be a laboratory genetic counselor—A qualitative exploration
Authors: Schwartz Lisa , Mackall Mia S. , Arjunan Aishwarya , Goodenberger McKinsey , Mills Rachel , Witherington Sarah 
Published: July 2023

AbstractOpportunities for genetic counselors to work in the laboratory have grown exponentially, yet the professional development needed to serve in these roles had not been previously explored. This study aimed to identify competencies required for entry‐level genetic counselors working in the laboratory, explore the perceived level of preparation of these competencies as noted by experts in the laboratories, and assess the perceived value of additional credentialing for genetic counselors practicing in these settings. Twenty genetic counselors working in the laboratory setting and five MD or PhD laboratory managers, identified through purposeful and snowball sampling and with at least 5 years of experience working in a laboratory, were interviewed using a semi‐structured protocol. Transcripts were analyzed thematically using deductive and inductive coding. Key findings included the distinction of laboratory and industry roles as involving nondirect patient care and differing from genetic counseling roles in the clinical setting. Genetic counselors working in the laboratory feel well prepared to transition into this setting and provide a unique patient‐focused perspective to laboratory roles, including variant interpretation, marketing, and product development. Practice‐based competencies (PBCs) were translatable to those used in the laboratory, yet variant interpretation, limitations of genomics‐based tests, and the business of health care were noted as important to these roles but not fully addressed in the PBCs. Additional skills were often developed through on‐the‐job training and interdisciplinary collaboration, but more exposure to diverse roles in genetic counseling programs' didactic and field training was recommended. The majority felt that requiring an additional post‐master's credential to work in the laboratory setting may restrict movement into these roles. Several questioned their identity as genetic counselors as they were no longer providing direct patient care and/or had been dissuaded by others from pursuing a laboratory position. Research focused on professional identity among genetic counselors working in nondirect patient care roles is warranted.
Group concept mapping – bridging the gap between conceptual papers and empirical research
Authors: Homer Stephen T. 
Published: July 2023

AbstractWith growing internationalism, there is a shift in research patterns in developing countries, especially China and India, generating vital and contemporary research areas that are beginning to challenge the existing Western‐dominated research literature in social sciences. Yet, many of the new ideas within conceptual papers by the social sciences are not empirically validated, let alone operationalized. This is where the group concept mapping method can play a role in bridging the gap between phenomenal conceptualization and having an empirically valid model that can then be operationalized. The group concept mapping process involves five steps: create statements, sort statements, run multidimensional scaling (MDS) of sorted units, run cluster analysis, and label the clusters. This approach allows for the collective thoughts of a pre‐defined group to be collected and organized into a tangible output with academic rigor. This paper offers an overview of the group concept mapping methodology, discussing the processes of the method, how the method can be utilized fully within the business and broader social science context, and the strengths, weaknesses, and practical implications of group concept mapping.
Human Histology after Structure Preservation Cochlear Implantation via Round Window Insertion
Authors: Geerardyn Alexander , Zhu MengYu , Klabbers Tim , Huinck Wendy , Mylanus Emmanuel , Nadol Joseph B. , Verhaert Nicolas , Quesnel Alicia M. 
Published: July 2023

ObjectivesCurrent surgical techniques aim to preserve intracochlear structures during cochlear implant (CI) insertion to maintain residual cochlear function. The optimal technique to minimize damage, however, is still under debate. The aim of this study is to histologically compare insertional trauma and intracochlear tissue formation in humans with a CI implanted via different insertion techniques.MethodsOne recent temporal bone from a donor who underwent implantation of a full‐length CI (576°) via round window (RW) insertion was compared with nine cases implanted via cochleostomy (CO) or extended round window (ERW) approach. Insertional trauma was assessed on H&E‐stained histological sections. 3D reconstructions were generated and virtually re‐sectioned to measure intracochlear volumes of fibrosis and neo‐ossification.ResultsThe RW insertion case showed electrode translocation via the spiral ligament. 2/9 CO/ERW cases showed no insertional trauma. The total volume of the cochlea occupied by fibro‐osseous tissue was 10.8% in the RW case compared with a mean of 30.6% (range 8.7%–44.8%, N = 9) in the CO/ERW cases. The difference in tissue formation in the basal 5 mm of scala tympani, however, was even more pronounced when the RW case (12.3%) was compared with the cases with a CO/ERW approach (mean of 93.8%, range 81% to 100%, N = 9).ConclusionsFull‐length CI insertions via the RW can be minimally traumatic at the cochlear base without inducing extensive fibro‐osseous tissue formation locally. The current study further supports the hypothesis that drilling of the cochleostomy with damage to the endosteum incites a local tissue reaction.Level of Evidence4: Case–control study Laryngoscope, 134:945–953, 2024
Fabrication of Zein‐Based Fibrous Scaffolds for Biomedical Applications—A Review
Authors: Rahman Mustafijur , Dip Tanvir Mahady , Haase Tina , Truong Yen Bach , Le Tu C. , Houshyar Shadi 
Published: July 2023

AbstractZein, which accounts for around 80% of the total protein composition in corn, is a biocompatible and biodegradable substance derived from renewable sources. Although insoluble in water, its amphiphilic characteristics are utilized to generate nanoparticles, nanofibers, microparticles, and even films. Numerous recent studies have demonstrated the potential of zein as a prospective biomaterial to develop fibrous scaffolds for biomedical functions owing to its biocompatibility, fibrous formation, and encapsulating qualities. Fabrication of zein‐based fibrous scaffolds for biomedical applications is achieved by a wide variety of techniques, including electrospinning, wet spinning, freeze drying, and additive manufacturing. This article overviews current advancements in manufacturing techniques for zein‐based fibrous scaffolds. In addition, it summarizes the most recent biomedical applications and research activities utilizing zein‐based fibrous scaffolds. Overall, zein is proposed as a potential biomaterial for the production of fibrous scaffolds that stimulate cell adhesion and proliferation in a number of exciting biomedical applications due to its biodegradability, biocompatibility, and other unique features related to its structure.
Targeting BRPF3 moderately reverses olaparib resistance in high grade serous ovarian carcinoma
Authors: Bitler Benjamin G. , Bailey Courtney A. , Yamamoto Tomomi M. , McMellen Alexandra , Kim Hyunmin , Watson Zachary L. 
Published: July 2023

AbstractPARP inhibitors (PARPi) kill cancer cells by stalling DNA replication and preventing DNA repair, resulting in a critical accumulation of DNA damage. Resistance to PARPi is a growing clinical problem in the treatment of high grade serous ovarian carcinoma (HGSOC). Acetylation of histone H3 lysine 14 (H3K14ac) and associated histone acetyltransferases (HATs) and epigenetic readers have known functions in DNA repair and replication. Our objectives are to examine their expression and activities in the context of PARPi‐resistant HGSOC, and to determine if targeting H3K14ac or associated proteins has therapeutic potential. Using mass spectrometry profiling of histone modifications, we observed increased H3K14ac enrichment in PARPi‐resistant HGSOC cells relative to isogenic PARPi‐sensitive lines. By reverse‐transcriptase quantitative PCR and RNA‐seq, we also observed altered expression of numerous HATs in PARPi‐resistant HGSOC cells and a PARPi‐resistant PDX model. Knockdown of HATs only modestly altered PARPi response, although knockdown and inhibition of PCAF significantly increased resistance. Pharmacologic inhibition of HBO1 depleted H3K14ac but did not affect PARPi response. However, knockdown and inhibition of BRPF3, a bromodomain and PHD‐finger containing protein that is known to interact in a complex with HBO1, did reduce PARPi resistance. This study demonstrates that depletion of H3K14ac does not affect PARPi response in HGSOC. Our data suggest that the bromodomain function of HAT proteins, such as PCAF, or accessory proteins, such as BRPF3, may play a more direct role compared to direct HATs function in PARPi response.
Senescence‐based colorectal cancer subtyping reveals distinct molecular characteristics and therapeutic strategies
Authors: Lv Min‐Yi , Cai Du , Li Cheng‐Hang , Chen Junguo , Li Guanman , Hu Chuling , Gai Baowen , Lei Jiaxin , Lan Ping , Wu Xiaojian , He Xiaosheng , Gao Feng 
Published: July 2023

AbstractCellular senescence has been listed as a hallmark of cancer, but its role in colorectal cancer (CRC) remains unclear. We comprehensively evaluated the transcriptome, genome, digital pathology, and clinical data from multiple datasets of CRC patients and proposed a novel senescence subtype for CRC. Multi‐omics data was used to analyze the biological features, tumor microenvironment, and mutation landscape of senescence subtypes, as well as drug sensitivity and immunotherapy response. The senescence score was constructed to better quantify senescence in each patient for clinical use. Unsupervised learning revealed three transcriptome‐based senescence subtypes. Cluster 1, characterized by low senescence and activated proliferative pathways, was sensitive to chemotherapeutic drugs. Cluster 2, characterized by intermediate senescence and high immune infiltration, exhibited significant immunotherapeutic advantages. Cluster 3, characterized by high senescence, high immune, and stroma infiltration, had a worse prognosis and maybe benefit from targeted therapy. We further constructed a senescence scoring system based on seven senescent genes through machine learning. Lower senescence scores were highly predictive of longer disease‐free survival, and patients with low senescence scores may benefit from immunotherapy. We proposed the senescence subtypes of CRC and our findings provide potential treatment interventions for each CRC senescence subtype to promote precision treatment.
Extension of the contour‐path effective‐permittivity finite‐difference time‐domain method to three‐dimensional problems for the analysis of arbitrarily shaped dielectric media
Authors: Takeya Kazuma , Iwamoto Tetsuya , Shibayama Jun , Yamauchi Junji , Nakano Hisamatsu 
Published: July 2023

AbstractThe conventional finite‐difference time‐domain (FDTD) method has been formulated in the Cartesian coordinate system. In this case, the staircase approximation should be used even for the analysis of arbitrarily shaped dielectric media. As a result, small meshes are required to accurately model these media. In this letter, we extend the contour‐path effective‐permittivity (CP‐EP) technique to three‐dimensional (3D) problems in which a reasonably accurate solution can be obtained with larger meshes for arbitrarily shaped dielectric media. The formulation is performed with the position of the dielectric medium being considered in each cell, leading to its effective permittivity. We compare the result from the present method with the Mie solution and that from the conventional staircase FDTD method to confirm the effectiveness of the 3D CP‐EP technique. The relative error of the scattering cross‐section is reduced to about half that of the staircase FDTD method.