bluuebunny/crossref_metadata_2025_split huggingface dataset:
Food taboos and animal conservation: a systematic review on how cultural expressions influence interaction with wildlife species
Authors: Landim André Santos , de Menezes Souza Jeferson , dos Santos Lucrécia Braz , de Freitas Lins-Neto Ernani Machado , da Silva Daniel Tenório , Ferreira Felipe Silva 
Published: July 2023

Abstract
Background
Human societies have food taboos as social rules that restrict access to a particular animal. Taboos are pointed out as tools for the conservation of animals, considering that the presence of this social rule prevents the consumption of animals. This work consists of a systematic review that aimed to verify how food taboos vary between different animal species, and how this relationship has influenced their conservation.

Methods
For this systematic review, the search for articles by keywords took place in the databases “Science Direct,” Scopus,” “SciELo” and “Web of Science,” associating the term “taboo” with the taxa “amphibians,” “birds,” “mammals,” “fish” and “reptiles.” From this search, 3959 titles were found related to the key terms of the research. After the entire screening process carried out by paired reviewers, only 25 articles were included in the search.

Results
It was identified that 100 species of animals are related to some type of taboo, and segmental taboos and specific taboos were predominant, with 93 and 31 citations, respectively. In addition, the taxon with the most taboos recorded was fish, followed by mammals. Our findings indicate that the taboo protects 99% of the animal species mentioned, being a crucial tool for the conservation of these species.

Conclusions
The present study covered the status of current knowledge about food taboos associated with wildlife in the world. It is noticeable that taboos have a considerable effect on animal conservation, as the social restrictions imposed by taboos effectively contribute to the local conservation of species.
Association between physical activity and bone mineral density in postmenopausal women: a cross-sectional study from the NHANES 2007–2018
Authors: Ji Jiazhong , Hou Yue , Li Zhaoyang , Zhou Ying , Xue Huaming , Wen Tao , Yang Tao , Xue Long , Tu Yihui , Ma Tong 
Published: July 2023

Abstract
Background
Physical activity (PA) is generally encouraged for the treatment of osteoporosis. However, epidemiological statistics on the level of physical activity required for bone health are scarce. The purpose of this research was to analyze the association between PA and total spine bone mineral density (BMD) in postmenopausal women.

Methods
The research study included postmenopausal women aged ≥ 50 from the National Health and Nutrition Examination Survey. The metabolic equivalent (MET), weekly frequency, and duration of each activity were used to calculate PA. Furthermore, the correlations between BMD and PA were investigated by multivariable weighted logistic regression.

Results
Eventually, 1681 postmenopausal women were included, with a weighted mean age of 62.27 ± 8.18 years. This study found that performing ≥ 38MET-h/wk was linked to a lower risk of osteoporosis after controlling for several covariates. Furthermore, the subgroup analysis revealed that the connection between total spine BMD and moderate-to-vigorous PA was more obvious among postmenopausal women aged < 65 years or individuals with normal BMI (< 25 kg/m2).

Conclusion
Physical activity ranging from moderate to vigorous was linked to higher total spine BMD in postmenopausal women.
Biceps-based 3-layer reconstruction of the irreparable rotator cuff: a technical note on biceps tendon as a common local graft for in-situ superior capsular reconstruction, partial cuff repair, and middle trapezius tendon transfer
Authors: Kandeel Amr Abdel-Mordy 
Published: July 2023

Abstract
Background
For irreparable rotator cuff tears, 3-layer tendon reconstruction (in which in-situ superior capsular reconstruction-reinforced partial rotator cuff repair was augmented with hamstring-sheet-lengthened middle trapezius tendon transfer) was recently reported to achieve satisfactory postoperative outcomes. To avoid hamstring graft-related drawbacks, the current note describes a technical modification of that reconstruct; wherein long head of biceps tendon is used as a cornerstone structure for simultaneously reconstructing the superior capsule; lengthening the transferred middle trapezius tendon; and augmenting the partially-repaired rotator cuff.

Methods
Via sub-pectoral approach, long head of biceps tendon is distally-tenotomized. Through McKenzie approach, proximal stump of the tenotomized long head of biceps is retrieved to the sub-acromial space where double-row biceps tenodesis (into a trough at the greater tuberosity) is performed for reconstructing the superior capsule. Next, postero-superior rotator cuff is partially repaired, and side-to-side sutured to the reconstructed capsule. Through a 7–8-cm skin incision over the medial scapular spine, middle trapezius tendon is released. Portion of long head of biceps tendon distal to the tenodesis site is retrieved via a sub-trapezius/sub-acromial corridor to the scapular wound where it is re-attached to the released middle trapezius tendon.

Results
Use of long head of biceps tendon as a common local graft (for simultaneously reconstructing the superior capsule, lengthening the transferred middle trapezius tendon, and augmenting the partially-repaired rotator cuff) is technically feasible provided that the harvested tendon stump is at least 10 cm in length.

Conclusion
While avoiding hamstring graft-related complications, the currently-reported biceps-based 3-layer rotator cuff tendon reconstruction might offer the advantages of reproducibility, safety, simplicity and quickness; however, it should be validated via further studies.
Trial registration The present study was approved by the Institutional Committee of Scientific Research and Ethics (3-2023Orth10-1).
Traditional Chinese Medicine Shi-Bi-Man regulates lactic acid metabolism and drives hair follicle stem cell activation to promote hair regeneration
Authors: Du Haojie , Zhang Tao , Wang Qiao , Cao Xinran , Zheng Huiwen , Li Jiabin , Zhu Jianxia , Qu Jiao , Guo Lehang , Sun Yang 
Published: July 2023

Abstract
Background
As a supplement for promoting hair health, Shi-Bi-Man (SBM) is a prescription comprising various traditional Chinese medicines. Though SBM has been reported to promote hair regeneration, its molecular mechanism remains unclear. Cynomolgus monkeys (Macaca fascicularis) are non-human primates with a gene expression profile similar to that of humans. The purpose of this research is to evaluate the effect of SBM on promoting hair regeneration in cynomolgus monkeys and to reveal the underlying mechanism.

Methods
The effect of SBM on hair regeneration was observed by skin administration on 6 cynomolgus monkeys with artificial back shaving. The molecular mechanism of SBM was studied using single-cell RNA sequencing (scRNA-seq) in combination with quantitative polymerase chain reaction (qPCR) detection for gene transcription level, and immunofluorescence staining verification for protein level.

Results
SBM significantly induced hair regeneration in cynomolgus monkeys, increased hair follicle number and facilitated hair follicle development. ScRNA-seq revealed an increase in the number of hair follicle stem cells (HFSCs) with a higher activation state, as evidenced by the higher expression of activation marker LDHA related to metabolism and the proliferation marker MKI67. Immunofluorescence analysis at the protein level and qPCR at the mRNA level confirmed the sequencing data. Cellchat analysis revealed an enrichment of ligand-receptor pairs involved in intercellular communication in Laminin-related pathways.

Conclusion
SBM significantly promotes hair regeneration in cynomolgus monkeys. Mechanically, SBM can up-regulate LDHA-mediated lactic acid metabolism and drive HFSC activation, which in turn promotes the proliferation and differentiation of HFSCs.
Primary healthcare delivery models in African conflict-affected settings: a systematic review
Authors: Omam Lundi-Anne , Jarman Elizabeth , O’Laughlin Kelli N. , Parkes-Ratanshi Rosalind 
Published: July 2023

Abstract
Background
In conflict-affected settings, access to primary healthcare for displaced populations is constrained by multiple challenges. These include geographical, cultural, communication, logistical and financial barriers, as well as risks posed to health workers and the population by insecurity. Different models of care are used to provide primary healthcare to affected communities. However, there is a paucity of evidence on how these models are selected and implemented by organisations working in conflict and displacement-affected settings. Our aim was to explore the different primary healthcare delivery models used in conflict-affected settings to understand gaps in existing healthcare delivery models.

Methods
We conducted a systematic review using the Preferred Reporting Items for Systematic Reviews and Meta-analyses guidelines. The review protocol was registered with the International Prospective Register of Systematic Reviews. We searched six databases for manuscripts published from January 1992 to December 2020. Publications were included if they reported primary healthcare models of care in conflict-affected settings of Africa. Data was analyzed descriptively and thematically using tables, charts and text.

Results
Forty-eight primary research articles were included for analysis from which thirty-three were rated as “high” quality. The results showed that the models of care in place in these conflict-affected settings include health facility-based, community-based, mobile clinics, outreach and home visits. Primary healthcare for internally displaced persons and refugees is provided by a wide range of actors including national and international organisations. A range of services is offered, most commonly nutrition, mental health and sexual/reproductive health. Some organisations offer vertical (stand-alone) services, while others use an integrated service delivery model. Multiple cadres of healthcare workers provide services, frequently lay healthcare workers such as Community Health Workers.

Conclusion
Understanding the different modalities of primary healthcare delivery in conflict-affected settings is important to identify existing practices and gaps in service delivery. Service delivery using community health workers in conflict-affected settings is a low-cost primary care delivery strategy that may help optimize contributions of existing personnel through task shifting.
Effectiveness of individualized counseling on the duration of exclusive breastfeeding: study protocol for a multicenter, randomized, parallel, and open clinical trial
Authors: Ruiz Mariana Torreglosa , Rodrigues Elisa da Conceição , da Silva Karine Emanuelle Peixoto Oliveira , de Resende Cynthya Viana , Cavalcanti Michele Curcino , dos Santos Luciano Marques , Wernet Monika , Gomes Ana Letícia Monteiro , Christoffel Marialda Moreira , Raponi Maria Beatriz Guimarães , da Silva Jéssica Aparecida , de Oliveira Jacqueline Faria , Contim Divanice , Linares Ana Maria 
Published: July 2023

Abstract
Background
Despite the benefits of breastfeeding, early weaning is a reality, so less than 50% of children worldwide and in Brazil are on exclusive breastfeeding in the sixth month of life. A strategy to counteract this scenario is breastfeeding counseling. This study aims to verify the effectiveness of individualized counseling by nurses trained in breastfeeding counseling, on the duration of exclusive breastfeeding, compared to standard care.

Methods
Multicenter, randomized, parallel, and open clinical trial, with primiparous women aged over 18 years, hospitalized in rooming-in wards at participating centers and hemodynamically stable, aware, and oriented, who had a single-fetus pregnancy and gave birth, regardless of the type of delivery, with live child, gestational age of 37 to 42 weeks and birth weight greater than 2500 g. The women will be initially approached in rooming-in wards and, upon consent to participate in the study, will be allocated through randomization by blocks composed of eight participants in two groups: intervention and control. The randomization lists will be organized by a central without involvement with the study, which will manage the allocation groups and be prepared in the Randon® program. Women allocated to the intervention group will receive breastfeeding counseling by trained nurses, and those in the control group will receive standard care at the center participating in the study.

Discussion
The results can contribute to breastfeeding by evidencing possible exclusivity and duration of the counseling trained nurses provide.

Trial registration
REBEC RBR-4w9v5rq (UTN: U1111-1284–3559) (https://ensaiosclinicos.gov.br/rg/RBR-4w9v5rq). Posted on March 20, 2023.
Chromatographic reversed HPLC and TLC-densitometry methods for simultaneous determination of serdexmethylphenidate and dexmethylphenidate in presence of their degradation products—with computational assessment
Authors: Kelani Khadiga M. , Nassar Ahmed M. W. , Omran Gamal A. , Morshedy Samir , Elsonbaty Ahmed , Talaat Wael 
Published: July 2023

AbstractTwo Chromatographic methods have been established and optimized for simultaneous determination of serdexmethylphenidate (SER.DMP) and dexmethylphenidate (DMP) in the presence of their degradation products. The first method is a reversed phase high performance liquid chromatography with diode array detection (HPLC–DAD). Isocratic separation was carried out on Waters X-bridge Shield RP18 column (150×3.9×5 μm particle size) using a mixture of 5 mM phosphate buffer (pH 5.5): acetonitrile (40:60, v/v) as a mobile phase, flow rate 1 mL/min and detection at 220 nm. The second method is a thin-layer chromatography (TLC)—densitometry method using methanol: chloroform (70:30, v/v) as a mobile phase and UV scanning at 220 nm. In HPLC method, the linearity range of SER.DMP was (2.5–25 μg/mL); with LOD (0.051 μg/mL) and LOQ (0.165 μg/mL) while for DMP was (2.5–25 μg/mL); with LOD and LOQ of (0.098 μg/mL) and (0.186 μg/mL), respectively. For TLC method the sensitivity range of SER.DMP was (5–25 μg/mL), LOD was (0.184 μg/spot), while LOQ was (0.202 μg/ spot) whereas for DMP the sensitivity range was (5–25 μg/mL) with LOD of (0.115 μg/ spot) and LOQ of (0.237 μg/ spot), respectively. SER.DMP was found to be equally labile to acidic and alkaline hydrolysis, whereas DMP was sensitive to acidic hydrolysis only. Both drugs were successfully determined in presence of acidic and basic degradants by the two developed methods (stability indicating assay method). Chromatographic separation of the degradation products was carried out on TLC aluminum silica plates 60 F254, as a stationary phase, using methanol: dichloroethane: acetonitrile (60:20:20 v/v), as a mobile phase. The degradation pathway was confirmed using TLC, IR, 1H-NMR and mass spectroscopy; moreover, the separation power was correlated to the computational results by applying molecular dynamic simulation. The developed methods were validated according to the International Conference on Harmonization (ICH) guidelines demonstrating good accuracy and precision. They were successfully applied for quantitation of SER.DMP and DMP in pure and capsule forms. The results were statistically compared with those obtained by the reported method in terms of accuracy, precision and robustness, and no significant difference was found.
Modification of conducting arylidene copolymers by formation of inclusion complexes: synthesis, characterization, and applications as highly corrosion inhibitors for mild steel
Authors: Dardeer Hemat M. , Abdel-Hakim Mohamed , Aly Kamal I. , Sayed Marwa M. 
Published: July 2023

AbstractModifying the metal surface is one solution to the industry’s growing corrosion problem. Thus, via threading approach and insertion of copolymers (CoP5-7) containing polyarylidenes through the internal cavity beta-cyclodextrin β-CD, novel pseudopolyrotaxanes copolymers (PC5-7) are developed, resulting in mild steel corrosion inhibition. Inhibitors of corrosion based on β-CD molecules adsorb strongly to metal surfaces because of their many polar groups, adsorption centers, many linkages of side chains, and benzene rings. The corrosion inhibition efficiencies IE % statistics have been revised via the Tafel polarization method and Spectroscopy based on the electrochemical impedance (EIS), with PC7 achieving the highest 99.93% in 1.0 M H2SO4; they are mixed-type inhibitors. The chemical composition of the resulting PCs is determined with Fourier transform infrared spectroscopy (FTIR), Scanning electron microscopy (SEM) is utilized to examine the morphological structure of the produced polymers, and X-ray diffraction is employed to identify crystallinity. Encapsulating CoP5-7 with β-CD changes the morphological structures and increases the generated PCs' crystallinity. The thermal stability of PCs is studied, indicating the presence of these CoPs within the β-CD cavities enhances their thermal stability. This research will be a stepping stone for developing high-efficiency anti-corrosion coatings and various industrial applications.
Transcriptional insights into Chlorella sp. ABC-001: a comparative study of carbon fixation and lipid synthesis under different CO2 conditions
Authors: Koh Hyun Gi , Cho Jun Muk , Jeon Seungjib , Chang Yong Keun , Lee Bongsoo , Kang Nam Kyu 
Published: July 2023

Abstract
Background
Microalgae's low tolerance to high CO2 concentrations presents a significant challenge for its industrial application, especially when considering the utilization of industrial exhaust gas streams with high CO2 content—an economically and environmentally attractive option. Therefore, the objectives of this study were to investigate the metabolic changes in carbon fixation and lipid accumulation of microalgae under ambient air and high CO2 conditions, deepen our understanding of the molecular mechanisms driving these processes, and identify potential target genes for metabolic engineering in microalgae. To accomplish these goals, we conducted a transcriptomic analysis of the high CO2-tolerant strain, Chlorella sp. ABC-001, under two different carbon dioxide levels (ambient air and 10% CO2) and at various growth phases.

Results
Cells cultivated with 10% CO2 exhibited significantly better growth and lipid accumulation rates, achieving up to 2.5-fold higher cell density and twice the lipid content by day 7. To understand the relationship between CO2 concentrations and phenotypes, transcriptomic analysis was conducted across different CO2 conditions and growth phases. According to the analysis of differentially expressed genes and gene ontology, Chlorella sp. ABC-001 exhibited the development of chloroplast organelles during the early exponential phase under high CO2 conditions, resulting in improved CO2 fixation and enhanced photosynthesis. Cobalamin-independent methionine synthase expression was also significantly elevated during the early growth stage, likely contributing to the methionine supply required for various metabolic activities and active proliferation. Conversely, the cells showed sustained repression of carbonic anhydrase and ferredoxin hydrogenase, involved in the carbon concentrating mechanism, throughout the cultivation period under high CO2 conditions. This study also delved into the transcriptomic profiles in the Calvin cycle, nitrogen reductase, and lipid synthesis. Particularly, Chlorella sp. ABC-001 showed high expression levels of genes involved in lipid synthesis, such as glycerol-3-phosphate dehydrogenase and phospholipid-diacylglycerol acyltransferase. These findings suggest potential targets for metabolic engineering aimed at enhancing lipid production in microalgae.

Conclusions
We expect that our findings will help understand the carbon concentrating mechanism, photosynthesis, nitrogen assimilation, and lipid accumulation metabolisms of green algae according to CO2 concentrations. This study also provides insights into systems metabolic engineering of microalgae for improved performance in the future.
Cryptosporidium mortiferum n. sp. (Apicomplexa: Cryptosporidiidae), the species causing lethal cryptosporidiosis in Eurasian red squirrels (Sciurus vulgaris)
Authors: Tůmová Lenka , Ježková Jana , Prediger Jitka , Holubová Nikola , Sak Bohumil , Konečný Roman , Květoňová Dana , Hlásková Lenka , Rost Michael , McEvoy John , Xiao Lihua , Santín Monica , Kváč Martin 
Published: July 2023

Abstract
Background
Cryptosporidium spp. are globally distributed parasites that infect epithelial cells in the microvillus border of the gastrointestinal tract of all classes of vertebrates. Cryptosporidium chipmunk genotype I is a common parasite in North American tree squirrels. It was introduced into Europe with eastern gray squirrels and poses an infection risk to native European squirrel species, for which infection is fatal. In this study, the biology and genetic variability of different isolates of chipmunk genotype I were investigated.

Methods
The genetic diversity of Cryptosporidium chipmunk genotype I was analyzed by PCR/sequencing of the SSU rRNA, actin, HSP70, COWP, TRAP-C1 and gp60 genes. The biology of chipmunk genotype I, including oocyst size, localization of the life cycle stages and pathology, was examined by light and electron microscopy and histology. Infectivity to Eurasian red squirrels and eastern gray squirrels was verified experimentally.

Results
Phylogenic analyses at studied genes revealed that chipmunk genotype I is genetically distinct from other Cryptosporidium spp. No detectable infection occurred in chickens and guinea pigs experimentally inoculated with chipmunk genotype I, while in laboratory mice, ferrets, gerbils, Eurasian red squirrels and eastern gray squirrels, oocyst shedding began between 4 and 11 days post infection. While infection in mice, gerbils, ferrets and eastern gray squirrels was asymptomatic or had mild clinical signs, Eurasian red squirrels developed severe cryptosporidiosis that resulted in host death. The rapid onset of clinical signs characterized by severe diarrhea, apathy, loss of appetite and subsequent death of the individual may explain the sporadic occurrence of this Cryptosporidium in field studies and its concurrent spread in the population of native European squirrels. Oocysts obtained from a naturally infected human, the original inoculum, were 5.64 × 5.37 μm and did not differ in size from oocysts obtained from experimentally infected hosts. Cryptosporidium chipmunk genotype I infection was localized exclusively in the cecum and anterior part of the colon.

Conclusions
Based on these differences in genetics, host specificity and pathogenicity, we propose the name Cryptosporidium mortiferum n. sp. for this parasite previously known as Cryptosporidium chipmunk genotype I.

Graphical Abstract