bluuebunny/crossref_metadata_2025_split huggingface dataset:
Immediate placement and restoration of implants combined with guided bone regeneration to rehabilitate the partially edentulous anterior mandible. A retrospective clinical study with an up to 8-year follow-up
Authors: Kolerman Roni , Rabie Hanan Abu , Sculean Anton D. , Liat Chaushu , Szmukler-Moncler Serge , Tagger-Green Nirit 
Published: June 2023

Abstract
Objectives: This retrospective study aimed to determine the survival and aesthetic outcomes of implants immediately placed and restored in the anterior mandible.
Materials and Methods: The study included 75 implants that were inserted along with guided bone regeneration in 42 patients. Over a follow-up period of 3 to 8 years (mean of 6.95 ± 1.78 years), the study evaluated aesthetic, marginal bone loss (MBL), and biological and prosthetic complications.
Results: No failures were recorded during the follow-up period; after eight years, the survival rate was 100%. Peri-implantitis was diagnosed in 6/29 (20.7%). Patient's age and gender did not have a statistically significant impact on MBL, but smokers had a greater MBL than non-smokers at the 8-year (2.98mm vs. 1.23mm, respectively, p = 0.016) time-point. At 3 years, only 13.3% of the implants had mesial papillae, 36.0% had distal papillae, and 16.0% had the cervical metallic part of the abutment exposed. Peri-implantitis was diagnosed in 20.7% of the patients at the 8-year follow-up time.
Conclusions: Immediate placement and restoration can be considered a feasible treatment option for replacing hopeless anterior mandibular teeth, provided that certain prerequisites are met. However, complete papillae formation may not be achieved.
Clinical relevance: Long-term evidence, on the feasibility of immediate implant placement and restoration of the mandibular incisors .
Particulate FDBA material and collagen membranes promoted successful wound healing in a trans-gingival environment. Nevertheless, complete papillae formation is not a feasible outcome.
The AL-ao379 gene plays a role in promoting the invasion stage of Bursaphelenchus xylophilus trapped by Arthrobotrys cladodes
Authors: Jia Hanqi , Wang Feng , Liu Xuefeng , Li Danlei 
Published: June 2023

Abstract
BACKGROUND: Nematode-trapping fungi can be used to develop specialized trapping devices to trap and kill nematodes, and the use of such fungi, with high efficiency and no toxicity to the environment, as biological control agents is very promising. Moreover, an understanding of the trap formation mechanism and the discovery of key pathogenic genes can help improve the efficacy of biocontrol agents.
RESULTS: In this study, we used RNA-Seq to reveal the transcriptome characteristics of Arthrobotrys cladodes under Bursaphelenchus xylophilus induction. When many traps were observed to be produced, mycelia were collected and subjected to differential expression analysis.
Differentially expressed genes were screened. AL-ao379 was identified by BLAST analysis and cloned by PCR. The results indicated that the AL-ao379 CDS was 1206 bp and encoded 402 amino acids. The expression of the AL-ao379 gene in different trapping stages was further compared by RT‒PCR. It was verified that the expression of the chitinase gene AL-ao379 increased significantly with the approach of the invasion stage and then decreased after reaching the highest levels in the invasion and predigestion stage.
CONCLUSION: The chitinase gene AO-379 has been shown to affect the trapping responses of A. oligospora in soils, but to our knowledge, the effect of the chitinase on arboreal nematode-trapping fungi has not been previously reported. Our results demonstrated that the AL-ao379 gene was a key gene and a potential control target involved in trapping and that it was significantly expressed in invasion stages. In addition, we proposed a model of infestation mechanism of B. xylophilus trapping by arboreal nematode-trapping fungi.
Increasing trend of antibiotic resistance in Shigella in Bangladesh: a plasmid-mediated transfer of mphA macrolide resistance gene
Authors: Asad Asaduzzaman , Jahan Israt , Munni Moriam Akter , Begum Ruma , Mukta Morium Akter , Saif Kazi , Faruque Shah Nayeem , Hayat Shoma , Islam Zhahirul 
Published: June 2023

Abstract
Shigellosis remains a common gastrointestinal disease mostly in children <5 years of age in developing countries. Azithromycin (AZM), a macrolide, is currently the first-line treatment for shigellosis in Bangladesh; ciprofloxacin (CIP) and ceftriaxone (CRO) are also used frequently. We aimed to evaluate the current epidemiology of antimicrobial resistance (AMR) and mechanism(s) of increasing macrolide resistance in Shigella in Bangladesh. A total of 2407 clinical isolates of Shigella from 2009 to 2016 were studied. Over the study period, Shigella sonnei was gradually increasing and become predominant (55%) over Shigella flexneri (36%) by 2016. We used CLSI-guided epidemiological cut-off value (ECV) for AZM in Shigella to set resistance breakpoints (zone-diameter ≤ 15 mm for S. flexneri and ≤ 11 mm for S. sonnei). Between 2009 and 2016, AZM resistance increased from 22% to approximately 60%, CIP resistance increased by 40%, and CRO resistance increased from zero to 15%. The mphA gene was the key macrolide resistance factor in Shigella; a 63MDa conjugative middle-range plasmid was harboring AZM and CRO resistance factors. Our findings show that, especially after 2014, there has been a rapid increase in resistance to the three most effective antibiotics. The rapid spread of macrolide (AZM) resistance genes among Shigella are driven by horizontal gene transfer rather than direct lineage.
Resveratrol and Quercetin Protect From Benzo(a)pyrene-induced Autophagy in Retinal Pigment Epithelial Cells
Authors: Kasikci Murat , Sen Serkan 
Published: June 2023

Abstract
Purpose
This study aims to investigate the role of Resveratrol (RES) and Quercetin (QR) treatments against Benzo(a)pyrene (B(a)p)-induced autophagy in retinal pigment epithelial cells.
Methods
The IC50 doses of B(a)p,RES and QR in retinal pigment epithelial cells were determined by MTT assay and the relevant agents were administered singly or in combinations to ARPE-19 cells for 24 hours. Occurrence of autophagy in the cells was verified by detection of autophagosomes under fluorescence microscope. Also, the mRNA expression levels of LC3 and Beclin 1 genes were analyzed by RT-PCR to collect further data on autophagy. Caspase-3 and IL-1β levels in lysed cells were analyzed by ELISA.
Results
Autophagosomes were detected in B(a)p-treated ARPE-19 cell lines, as well as a 1.787-fold increase in LC3 mRNA expression levels. No autophagosome occurred in RES and QR treatments, and a significant decrease in theirpercentage amounts were observed in B(a)p + RES and B(a)p + QR. The mRNA expression levels of LC3 and Beclin 1 also supported these findings.B(a)p had no effect on Caspase-3 levels in ARPE-19 cells, but combined with RES and QR, it increased Caspase-3 levels significantly.IL-1β levels were higher in B(a)p, B(a)p + QR, B(a)p + RES, RES and QR than control group. This rise in IL-1β levels was correlated with suppression of mRNA expression levels of Beclin 1.
Conclusion
B(a)p exposure caused autophagy in ARPE-19 cells, but did not induce apoptosis. RES and QR treatmentsprevented B(a)p-induced autophagy. Therefore, RES and QR treatments showedprotective effect against potential degenerative diseases caused by chronic exposure to B(a)p.
Nurse-patient Relationships: Implications for Retention in the PMTCT of HIV Programme
Authors: Abraham Susanna Aba , Clow Sheila Elizabeth 
Published: June 2023

Abstract
Background
Relationships established between nurses and midwives, and their patients have far-reaching implications; the most significant being its impact on the health-related outcome of patients.
These relationships are especially relevant in the Prevention of Mother-to-Child Transmission (PMTCT) of Human Immunodeficiency Virus (HIV) programme as women, diagnosed with HIV navigate the emotional and psychological effects of their diagnosis while carrying pregnancies. This study aimed to explore the relationships between nurses, midwives and mothers diagnosed with HIV and its impact on retention in the PMTCT Programme.
Methods
An Appreciative Inquiry approach that employed qualitative research methods was conducted among twenty-four participants made up of 12 HIV positive mothers, and eight midwives and four community health nurses engaged in the PMTCT programme. Individual generative interviews were conducted among the mothers while paired interviews were conducted among the health professionals. Thematic analysis guided by Colaizzi’s approach was conducted.
Results
Three main themes emerged; Establishing Rapport, Journeying Together and Celebrating the Spoils. Nurse-patient relationships evolved as the women progressed along the PMTCT cascade. Establishing trust, setting mutuals goals, sharing emotional experiences and personal stories, and building clients’ self-worth positively impacted commitment to the nurse-patient relationship and retention in the programme. Termination of care was unsuccessful in some relationships as professional boundaries were blurred.
Conclusions
The nurse-patient relationship in the PMTCT programme evolved as the relationship progressed along the PMTCT cascade. Strengthening of the nurse-patient relationships was underscored by building trust through maintenance of confidentiality, setting mutuals goals, shared emotional experiences and personal stories, and building clients self-worth. Therefore, there is a need to ensure that professional boundaries are set and maintained to reduce the occurrence of overdependence of the clients and burnout of the nurses.
Selection of M7G-related lncRNAs in kidney renal clear cell carcinoma and their prediction of immunotherapeutic efficacy
Authors: Zhong Shuangze , Chen Shangjin , Lin Hansheng , Luo Yuancheng , He Jingwei 
Published: June 2023

Abstract
Background:
Kidney renal clear cell carcinoma (KIRC) is a common malignant tumor of the urinary system. This study aims to develop new biomarkers for KIRC and explore the impact of biomarkers on the immunotherapeutic efficacy for KIRC, providing a theoretical basis for the treatment of KIRC patients.
Methods:
Transcriptome data for KIRC was obtained from the The Cancer Genome Atlas (TCGA) and International Cancer Genome Consortium (ICGC) databases. Weighted gene co-expression network analysis identified KIRC-related modules of long noncoding RNAs (lncRNAs). Intersection analysis was performed differentially expressed lncRNAs between KIRC and normal control samples, and lncRNAs associated with N(7)-methylguanosine (m7G), resulting in differentially expressed m7G-associated lncRNAs in KIRC patients (DE-m7G-lncRNAs). Machine Learning was employed to select biomarkers for KIRC. The prognostic value of biomarkers and clinical features was evaluated using Kaplan-Meier (K-M) survival analysis, univariate and multivariate Cox regression analysis. A nomogram was constructed based on biomarkers and clinical features, and its efficacy was evaluated using calibration curves and decision curves. Functional enrichment analysis was performed to investigate the functional enrichment of biomarkers. Correlation analysis was conducted to explore the relationship between biomarkers and immune cell infiltration levels and common immune checkpoint in KIRC samples.
Results:
By intersecting 575 KIRC-related module lncRNAs, 1773 differentially expressed lncRNAs, and 62 m7G-related lncRNAs, we identified 42 DE-m7G-lncRNAs. Using XGBoost and Boruta algorithms, 8 biomarkers for KIRC were selected. Kaplan-Meier survival analysis showed significant survival differences in KIRC patients with high and low expression of the PTCSC3 and RP11-321G12.1. Univariate and multivariate Cox regression analyses showed that AP000696.2, PTCSC3 and clinical characteristics were independent prognostic factors for patients with KIRC. A nomogram based on these prognostic factors accurately predicted the prognosis of KIRC patients. The biomarkers showed associations with clinical features of KIRC patients, mainly localized in the cytoplasm and related to cytokine-mediated immune response. Furthermore, immune feature analysis demonstrated a significant decrease in immune cell infiltration levels in KIRC samples compared to normal samples, with a negative correlation observed between the biomarkers and most differentially infiltrating immune cells and common immune checkpoints.
Conclusion:
In summary, this study discovered eight prognostic biomarkers associated with KIRC patients. These biomarkers showed significant correlations with clinical features, immune cell infiltration, and immune checkpoint expression in KIRC patients, laying a theoretical foundation for the diagnosis and treatment of KIRC.
Evaluation of Downy Mildew Resistance in Spinach (Spinacia oleracea)
Authors: Olaoye Dotun , Bhattarai Gehendra , Feng Chunda , Correll Jim , Shi Ainong 
Published: June 2023

Abstract
Spinach (Spinacia oleracea) is an economically important leafy vegetable grown in the United States and world-wide. The downy mildew pathogen, Peronospora effusa (Pfs), is a major biotic constraint impacting spinach production and quality. The use of resistant cultivars is an economical management option especially in organic production systems. As new races of the pathogen continue to appear, there is a need to continue to select for resistance to the emerging races. The objectives of this study were to evaluate a set of spinach hybrids and F2 breeding populations for resistance to Pfs race 5 to develop a better understanding of the genetics of downy mildew resistance. Also, we screened 39 commercial spinach cultivars for resistance to a recently identified race, Pfs race 19. The genetics of resistance to Pfs 5 was determined by greenhouse inoculations of F1 progeny (individual crosses between near isogenic lines, NIL3 or NIL1 and susceptible genotype, Viroflay) and F2 population progeny (cross between Califlay and susceptible Viroflay). Two hybrids were examined for resistance to Pfs 5. The results indicated that resistance conferred at the RPF1 or the RPF3 loci in a heterozygous (Rr) condition to Pfs 5 was completely dominant. Also, Chi-square analysis of the segregation pattern in the F2 population showed that resistance to Pfs race 5 was conferred by a single dominant gene. A total of 22 out of 39 spinach commercial cultivars were resistant to the newly reported Pfs race 19 and could be used in breeding programs to develop new cultivars with resistance to Pfs 19.
Global estimates on the number of people blind or visually impaired by glaucoma: A meta-analysis from 2000 to 2020
Authors: Bourne Rupert , Bourne Rupert 
Published: June 2023

Abstract
Objectives: To estimate global and regional trends from 2000 to 2020 of the number of persons visually impaired by glaucoma and their proportion of the total number of vision-impaired individuals.
Methods: A systematic review and meta-analysis of published population studies and grey literature from 2000 to 2020 was carried out to estimate global and regional trends. Moderate or severe vision loss (MSVI) was defined as a visual acuity of 6/60 or better but <6/18 (moderate vision loss) and visual acuity of 3/60 or better but <6/60 (severe vision loss). Blindness was defined as presenting visual acuity <3/60 or less than 10° visual field around central fixation.
Results: Globally, in 2020, 3.61 million people were blind and nearly 4.14 million were visually impaired by glaucoma. Glaucoma accounted for 8.39% (95% uncertainty intervals [UIs]: 6.54, 10.29) of all blindness and 1.41% (95% UI: 1.10, 1.75) of all MSVI. Regionally, the highest proportion of glaucoma-related blindness was found in high-income countries (26.12% [95% UI: 20.72, 32.09]), while the region with highest age-standardized prevalence of glaucoma-related blindness and MSVI was Sub-Saharan Africa. Between 2000 and 2020, global age-standardized prevalence of glaucoma-related blindness among adults ≥50 years decreased by 26.06% among males (95% UI: 25.87, 26.24), and by 21.75% among females (95% UI: 21.54, 21.96), while MSVI due to glaucoma increased by 3.7% among males (95% UI: 3.42, 3.98), and by 7.3% in females (95% UI: 7.01, 7.59).
Conclusions: Within the last two decades glaucoma has remained a major cause of blindness globally and regionally.
Evaluation of Microphysical Schemes of High-Resolution WRF-ARW Model in Windspeed Forecasting over a Complex Terrain Wind-farm Cluster in India
Authors: Choudhury Devanil , Keshav Bibhuti Sharan 
Published: June 2023

Abstract
Microphysical sensitivity experiments were conducted to forecast complex terrain windspeed over a windfarm cluster in Maharashtra, India using high-resolution WRF-ARW model with 6 km outer and 2 km inner domain during the month of June 2022. The simulations were compared to hub-height wind measurements from wind turbines data. Results showed that the WSM5 microphysical scheme produced the minimum absolute error for complex mountainous terrain, while Ferrier performed the worst, and produced the largest error in wind speed at a height of 120 meters. Moreover, planetary boundary layer and topographic representation also play a vital role in modeling complex terrain wind forecasts. The performance of other physical variables for different microphysical schemes remained almost similar with minor fluctuations. Our experiments suggest that the adopting high-resolution WRF-ARW model with suitable combinations of physical parameterizations especially WSM5 microphysical schemes can significantly improve windspeed forecasting over complex terrain wind sites.
Hemostatic capability of ultrafiltrated fresh frozen plasma compared to cryoprecipitate
Authors: Ichikawa Junko , Iba Toshiaki , Okazaki Ryouta , Fukuda Tomoki , Kodaka Mitsuharu , Komori Makiko , Levy Jerrold H. 
Published: June 2023

Abstract
This in vitro study evaluated the potential hemostatic effect of fresh frozen plasma (FFP) ultrafiltration on clotting factors, coagulation parameters, and plasma properties. ABO-specific units of FFP (n = 40) were prepared for the concentrated FFP and cryoprecipitate. Plasma water was removed from FFP by ultrafiltration using a dialyzer with a pump running at a 300 ml/min. The aliquot of each concentrated FFP after 50, 100, 200, and 250 ml of fluid removal were served for the standard coagulation assay, measurement of clotting activity, and plasma properties to compare those parameters of cryoprecipitate. Concentrated FFP contained 36.5% of fibrinogen in FFP with a mean concentration of 7.2 g/L, lower than the cryoprecipitate level. The levels of factor Ⅷ (FⅧ), von Willebrand factor (vWF): antigen (Ag), and vWF: ristocetin cofactor (Rco) were also lower in concentrated FFP, whereas the levels of factor Ⅴ, factor Ⅸ, factor ⅩⅢ, antithrombin and albumin was higher in concentrated FFP. Maximum clot firmness (MCF) in thromboelastometry was approximately one-half of that in cryoprecipitate. Although the levels of vWF: Ag, vWF: Rco, and FⅧ differed depending on the ABO blood types, fibrinogen levels, and MCF were not significantly different among the ABO blood groups in FFP and concentrated FFP.
Alterations of the intestinal microbiome and metabolome in women with rheumatoid arthritis
Authors: Yun Haifeng , Wang Xinxin , Wei Changjiang , Liu Qiuhong , Yan Lixian , Li Na , Zhang Guoxing , Cui Dawei , Liu Rui 
Published: June 2023

Abstract
Objectives: Rheumatoid arthritis (RA) is more common in women, and many reports of sex differences have been reported in various aspects of RA. However, there has been a lack of specific research on women's gut flora. To assess the association between the gut flora and RA patients, this study combined the microbiome with metabolomics.
Methods: Fecal samples from RA patients and healthy controls were collected for 16S rRNA sequencing. Nontargeted liquid chromatography‒mass spectrometry was used to detect metabolites in fecal samples. We comprehensively used various analytical methods to reveal changes in intestinal flora and metabolites in female patients.
Results: The gut flora of RA patients was significantly different from that of healthy women. The abundance of Bacteroides, Megamonas and Oscillospira was higher in RA patients, while the abundance of Prevotella, Gemmiger and Roseburia was lower than that of healthy women. Gemmiger, Bilophila and Odoribacter represented large differences in microflora between RA and healthy women and could be used as potential microorganisms in the diagnosis. Fatty acid biosynthesis was significantly different between RA patients and healthy women in terms of metabolic pathways. There were different degrees of correlation between the gut flora and metabolites. Lys-Phe-Lys and heptadecasphin-4-enine can be used as potential markers for RA diagnosis. There was an extremely significant positive correlation between Megamonas, Dialister and rheumatoid factors (RF), which was found for the first time.
Conclusions: These findings indicated that alterations of these gut microbiome and metabolome may contribute to the diagnosis and treatment of RA patients.
The Antidepressant Effect of Magnolol on Depression-like Behavior of CORT-treated Mice
Authors: Xu Chi , Ye Jiayu , Sun Yanting , Sun Xiujian , Liu Jing-gen 
Published: June 2023

Abstract
Although the antidepressant effect of magnolol has been revealed in previous reports, the mechanism remains unclear. In this study, the antidepressant effect of magnolol on corticosterone-induced (CORT-induced) depressed mice was investigated in vivo. After 21 days of CORT induction, the mice showed marked depressive-like behaviors, with a decrease in sucrose preference score and an increase in immobility time in tail suspension test (TST) and forced swimming test (FST). Pretreatment with either magnolol (50 mg/kg, i.p.) or the kappa opioid receptor (KOR) antagonist nor-BNI (10 mg/kg, i.p.) prevented CORT-induced depression-like behavior and reduced CORT-induced dynorphin (DYN A) elevation in the hippocampal DG. However, no depression-like behavior was observed in mice with KOR downregulation in the DG. We further found that upregulation of DYN A in the DG caused depression, which was blocked by intraperitoneal injection of nor-BNI and modulated by magnolol. The present study demonstrated that magnolol could ameliorate CORT-induced depression-like behaviors, by modulating the DYN A/KOR system in the DG of the hippocampus.
Suicide deaths during the COVID-19 pandemic in the United States, by region, gender and age, March 1, 2020-June 30, 2022
Authors: Faust Jeremy , Renton Benjamin , Du Chengan , Shah Sejal , Chen Alexander , Li Shu-Xia , Lin Zhenqiu , Black Tyler , Krumholz Harlan 
Published: June 2023

Abstract
The COVID-19 pandemic imposed substantial mental health stressors leading to concerns about an increased suicide risk. To investigate this issue, we investigated suicide mortality rates in the United States from March 1, 2020, through June 30, 2022, comparing them with data from the pre-pandemic period of January 2015 through February 2020. Suicide mortality in the United States was 3% below expected levels during the study period. However, there was an increased suicide incidence in adults ages 18–34 years. The concerns that the pandemic contributed to an overall marked increase in suicide risk is not supported by this analysis, but young adults did experience an increase.