bluuebunny/crossref_metadata_2025_split huggingface dataset:
Distribusi Jenis Asing Invasif Melothria pendula L. (Cucurbitaceae) di Kawasan Konservasi Ilmiah Kebun Raya Bogor
Authors: Kekung Cristina Mega Meyfiani , Husaini Iin Pertiwi A , Turhadi Turhadi 
Published: January 2025

Melothria pendula is belong to Cucurbitaceae family and originates from America continent. Previous research reported findings of M. pendula in several regions in Indonesia, such as in Bogor Botanical Gardens (KRB) as invasive species. The appearance of M. pendula in KRB has not yet been inventoried. As invasive species, M. pendula has the potential to be a threat to collection and native plants. This research aims to report the distribution of M. pendula in the scientific conservation area of KRB and measure the abiotic factors in the locations where M. pendula was found. This research was exploratory method which was carried out by exploring and morphological characterization of M. pendula. Measurement of abiotic factors, including temperature, air humidity, light intensity, and wind speed at each site where M. pendula was found. QGIS is used to mapping the distribution of M. pendula. Based on this study showed that there were 40 individuals of M. pendula which found distributed randomly in 15 sites (VG, VD, VB, XXIVA, IIM, IIC, IIP, XVI.VII, IVF, XXIVB, IIO, XVF, XXIIA, IIK, and seedling area) in KRB. M. pendula habitats in KRB characterized by forest land cover type with temperature of 31.1 ± 0.96 ℃, air humidity of 69 ± 2.73%, light intensity 3183.9 ± 3095 lux, and wind speed 0.7 ± 0.51 m/s.
Evaluasi Kemampuan Literasi Biodiversitas Mahasiswa
Authors: Fajri Siti Rabiatul , Citrawathi Desak Made , Adnyana Putu Budi , Arnyana Ida Bagus Putu 
Published: January 2025

Indonesia is one of the countries with the highest biodiversity in the world. However, this high diversity requires individual awareness and responsibility to maintain and utilize it sustainably. Therefore, biodiversity literacy is an important element, especially for students as prospective future generations who will play a role in natural resource management in the future. This study aims to evaluate the level of biodiversity literacy of students of the Biology study program at the University of Education Mandalika, by assessing six dimensions of biodiversity literacy: Conservation and Importance of Biodiversity (CIB), Ethics and Biodiversity (EB), Sustainability and Biodiversity (SB), Taking Action to Protect Biodiversity (TAPB), Biodiversity and Utility (BU), and Conservation and Importance of Species (CIS). The method used was quantitative descriptive, involving 50 students as respondents. The instrument used consisted of 27 items that were analyzed using One Way ANOVA to determine the average score and significant differences based on gender and student scores. The results showed that the average score of biodiversity literacy obtained a score of 2.08 with a less significant category (Low Biodiversity Literacy). Most of the indicators are at an average value below 2.50, only a small number of indicators have an average value above 2.50. In the CIB dimension, only 3 indicators have an average value of > 2.50 out of 5 indicators. In the TAPB dimension, only 1 indicator has an average > 2.50. While in other dimensions, the average value < 2.50. In addition, there was no significant difference in student scores based on gender. Thus, student biodiversity literacy still needs to be improved, especially in the aspects of real action and individual responsibility.
Development of Cooperative Learning-Based Student Worksheets (LKPD) on Chemical Nomenclature Subject Material for 10th Grade Students
Authors: Septiana Tri Merry , Sartika Rody Putra , Rasmawan Rahmat 
Published: January 2025

AbstractResearch has been done to develop a product in the form of student worksheets chemical compounds for SMA/MA grade X. This research aims to develop students ' worksheets of material nomenclature for SMA/MA grade X, knowing the quality of teaching materials based on expert test (expert judgement), knowing student response, and knowing the implementation of learning process. This research and development (R&D) is conducted based on 4-D models that are limited to the develop stage. The subject of this study is the LKPD based on cooperative learning in compound nomenclature. The product is in the quality of one chemical teacher, one Indonesian teacher, and two chemistry lecturers. Based on the results of the expert quality assessment, the LKPD based cooperative learning in compound name matter got very good/very high category with a percentage of 84.27% worth using as a teaching material in the learning process. Keywords: LKPD, Cooperative Learning, feasibility of the product
Characterization of Activated Charcoal Produced Using Green Chemistry Principle Approach Number 7
Authors: Thohir Muhammad Bakhru , Wijaya Terry Esi Putri 
Published: January 2025

Charcoal is an important component in the effort to provide clean water. One of the water qualities that need to be considered is the level of hardness. Reducing the level of hardness can be done with activated charcoal. The production of activated charcoal uses many synthetic materials. Therefore, aim of research is to produce, test the performance, and characterization of activated charcoal made with natural acids. The stages of this research are charcoal preparation and natural acid activator, carbonization, activation with natural acids (Averrhoa bilimbi L. and Citrus aurantifolia S.), and characterization. The production results were tested for adsorbing ability and characterization using FTIR, SEM, and XRD to determine the functional groups, structure, and components. The results showed that activated charcoal produced using natural acids had better performance in adsorbing. Characterization with FTIR showed the absorption of C-H functional groups shown at 785 cm-1, 1100 cm-1 for C-O functional groups, 3368 cm-1 for O-H stretching vibration groups. Typical active charcoal groups were also detected at wave numbers 1704 cm-1for C=O functional groups and 1575 cm-1 for C=C groups. Characterization with XRD showed the same pattern between charcoal produced with natural acids and the control. SEM images showed clear pores on the surface of the activated charcoal. Thus, natural acids can really be a candidate for activator replacement in the production of activated charcoal that applies the principles of green chemistry.
Antioxidant Activity of Avocado Folium (Persea americana M.)
Authors: Wusurwut Fahriya , Hasnaeni Hasnaeni , Waris Risda 
Published: January 2025

Free radicals can be formed through normal cell metabolism events, malnutrition and as a result of responses to external influences such as pollution and ultraviolet rays. Free radicals can cause premature aging, therefore antioxidants are needed to prevent premature aging. Avocado folium (Persea americana M.) contain secondary metabolite compounds, namely alkaloids, saponins, tannins, glycosides and flavonoids in the form of quercetin, where flavonoid compounds can act as antioxidants. This study aims to determine the antioxidant activity of avocado leaf extract (P. americana M), using the (2,2-dihphenyl-1-picrylhdrazil) (DPPH) method. The sample used in this research was avocado folium obtained from Southeast Maluku, Maluku Province). DPPH method with a UV-Vis spectrophotometer at a wavelength of 514 nm. The antioxidant activity of ethanol extract of avocado folium in this test is 112.410 µg / mL. The results of this study show that avocado folium extract has moderate antioxidant activity.
Prevalence and predictive factors of testosterone-induced erythrocytosis: a retrospective single center study
Authors: Neidhart Anina , von Wyl Viktor , Käslin Benno , Henzen Christoph , Fischli Stefan 
Published: January 2025

AimThis study analyzes the prevalence and predictive factors of testosterone-induced erythrocytosis (TIE) in patients receiving testosterone replacement therapy (TRT).MethodsRetrospective single-center observational study.Results247 patients were included; median age was 47.0 years (interquartile range (IQR) 32-60) and median follow-up years 2.9 (1.0-5.5). The most common indication for TRT was central hypogonadism (51%) followed by primary hypogonadism (26%). TRT was carried out with testosterone undecanoate (TU) n=194, testosterone enanthate (TE) n=18 and testosterone gel (n=35). Compared to baseline, hematocrit (HCT) values at last follow-up (LFU) increased significantly by +0.04 (95% confidence interval (CI) [0.027, 0.050], p=&lt;0.0001) in all patients (n=92) and +0.06 (95%CI [0.031, 0.057], p&lt;0.0001) in the TU group (n=71). 57% of the patients reached an HCT value&gt;0.46, 23% &gt;0.5 and 5%&gt;0.54. 46% of the patients who have reached an HCT value &gt;0.46 have had their highest HCT measurement within the first year of TRT application. Logistic regression analysis indicated that body mass index (BMI) was significantly associated with the development of an HCT ≥0.5 (p=0.013) and HCT ≥0.46 (p=0.008). There was an association between the baseline HCT measurement and the outcome of a HCT measurement ≥0.46 (p=0.025), patients with high starting values were more likely to develop TIE.ConclusionsTIE appears to be frequent and does not only present within the first year of therapy which indicates a close follow-up of laboratory values within the first year followed by annual controls. Baseline BMI and baseline HCT measurement should be considered in risk stratification of TIE development.
Suppression of MCP-1, IFN-γ and IL-6 production of HNSCC ex vivo by pembrolizumab added to docetaxel and cisplatin (TP) exceeding those of TP alone is linked to improved survival
Authors: Wellhausen Jana , Röhl Louisa , Berszin Michael , Krücken Irene , Zebralla Veit , Pirlich Markus , Stoehr Matthaeus , Wiegand Susanne , Dietz Andreas , Wald Theresa , Wichmann Gunnar 
Published: January 2025

BackgroundAdding pembrolizumab, an anti-PD-1 antibody approved for treatment of head and neck squamous cell carcinoma (HNSCC) to neoadjuvant (induction-) chemotherapy utilizing docetaxel and cisplatin (TP) followed by radiotherapy may improve outcome in larynx organ-preservation (LOP) that is investigated in the European Larynx-Organ preservation Study (ELOS). As biomarkers for response to TP and pembrolizumab +TP are missing but may include cytokines, this work aims on determining cytokines potentially linked to outcome as prognostic markers sufficient to predict and/or monitor response to successful LOP.MethodsCollagenase IV digests were generated from 47 histopathological confirmed HNSCC tumor samples and seeded in 96-well plates containing pembrolizumab, docetaxel, cisplatin either solely or in binary or ternary combination. According to the FLAVINO protocol, supernatants were collected after 3 days, adherent cells fixed using ethanol, air-dried and pan-cytokeratin positive epithelial cells counted using fluorescence microscopy. The cytokines IL-6, IL-8, IFN-γ, IP-10, MCP-1, TNF-α, and VEGF in the supernatant were quantified by sandwich ELISA.ResultsThe mode of interaction between pembrolizumab and TP was assessed and correlated to outcome (overall, disease-specific and progression-free survival of patients). Suppression of MCP-1, IFN-γ and IL-6 production by pembrolizumab + TP exceeding the suppressive effect of TP was detected in the majority of samples and linked to improved survival. Multivariate Cox proportional hazard regression modeling revealed MCP-1, IFN-γ and IL-6 as independent outcome predictors.ConclusionsComparing response to TP vs. pembrolizumab vs. TP + pembrolizumab may allow for identification of patients with superior outcome independent from treatment applied.
Acute kidney injury and ANCA positivity in a patient treated with glecaprevir/pibrentasvir: a case report
Authors: Kwon Lawrence Hyun , Griffiths Jennifer , DiFranza Lanny 
Published: January 2025

BackgroundGlecaprevir/pibrentasvir is an effective antiviral therapy for hepatitis C virus infection and is generally regarded safe in patients with renal impairment. However, renal complications are a notable, albeit rare, concern.Case presentationWe report a case of acute kidney injury in a man in his 50s with chronic hepatitis C virus, chronic obstructive pulmonary disease, morbid obesity, a history of heroin dependence, and untreated type 2 diabetes mellitus. About four weeks into an eight-week glecaprevir/pibrentasvir regimen he developed progressive lower extremity edema, bullae, and skin ulcers with worsening renal function. His serum creatinine rose to 4.46 mg/dL and blood urea nitrogen to 44 mg/dL. ANCA serology revealed dual perinuclear and cytoplasmic positivity, though anti-proteinase 3 and anti-myeloperoxidase antibody tests were negative. Kidney biopsy revealed diffuse tubulointerstitial injury with erythrocyte casts indicative of glomerular bleeding into the distal nephrons, though without glomerular crescent formation.ConclusionThis case illustrates the potential for glecaprevir/pibrentasvir to induce acute kidney injury, acute interstitial nephritis and possibly ANCA-associated vasculitis. Recognizing these adverse renal effects is critical for making timely diagnosis and management in hepatitis C virus patients undergoing antiviral therapy.
Evolution of artificial intelligence in healthcare: a 30-year bibliometric study
Authors: Xie Yaojue , Zhai Yuansheng , Lu Guihua 
Published: January 2025

IntroductionIn recent years, the development of artificial intelligence (AI) technologies, including machine learning, deep learning, and large language models, has significantly supported clinical work. Concurrently, the integration of artificial intelligence with the medical field has garnered increasing attention from medical experts. This study undertakes a dynamic and longitudinal bibliometric analysis of AI publications within the healthcare sector over the past three decades to investigate the current status and trends of the fusion between medicine and artificial intelligence.MethodsFollowing a search on the Web of Science, researchers retrieved all reviews and original articles concerning artificial intelligence in healthcare published between January 1993 and December 2023. The analysis employed Bibliometrix, Biblioshiny, and Microsoft Excel, incorporating the bibliometrix R package for data mining and analysis, and visualized the observed trends in bibliometrics.ResultsA total of 22,950 documents were collected in this study. From 1993 to 2023, there was a discernible upward trajectory in scientific output within bibliometrics. The United States and China emerged as primary contributors to medical artificial intelligence research, with Harvard University leading in publication volume among institutions. Notably, the rapid expansion of emerging topics such as COVID-19 and new drug discovery in recent years is noteworthy. Furthermore, the top five most cited papers in 2023 were all pertinent to the theme of ChatGPT.ConclusionThis study reveals a sustained explosive growth trend in AI technologies within the healthcare sector in recent years, with increasingly profound applications in medicine. Additionally, medical artificial intelligence research is dynamically evolving with the advent of new technologies. Moving forward, concerted efforts to bolster international collaboration and enhance comprehension and utilization of AI technologies are imperative for fostering novel innovations in healthcare.
Superiority of native seed core microbiomes in the suppression of bacterial wilt disease
Authors: Dong Yanling , Gong Jie , Yang Lei , Jiang Qipeng , Wen Chengzhi , Zhang Jidan , Yang Ruiyu , Wang Yao , Dai Yuhao , Gao Gui , Li Shili , Cao Yi , Ding Wei 
Published: January 2025

IntroductionNative endophytic microorganisms in tobacco seeds are closely related to their resistance to Ralstonia solanacearum (R. solanacearum) infections. However, the role of the native seed core microbiome in the suppression of bacterial wilt disease (BWD) remains underexplored.MethodsThe characteristics of endophytic bacterial communities in both resistant and susceptible tobacco varieties were characterized using high-throughput sequencing technology.ResultsThis study found Paenibacillus as a potential microbial antagonist against BWD based on its significantly greater presence in BWD-resistant tobacco varieties, with a relative abundance that was 83.10% greater in the seeds of resistant tobacco than in those of susceptible varieties. Furthermore, a Paenibacillus strain identified as Paenibacillus odorifer 6036-R2A-26 (P. odorifer 26) was isolated from the seeds of the resistant variety. Following irrigation treatment with P. odorifer 26, the BWD index was reduced by 51.08%. Additionally, this strain exhibited significant growth-promoting effects on tobacco. It significantly increased the fresh weight of the tobacco plants by 30.26% in terms of aboveground weight, 37.75% in terms of underground weight, and 33.97% in terms of aboveground dry weight. This study highlights the critical role of Paenibacillus in tobacco seeds in the suppression of BWD, which may result from its antagonistic and growth-promoting properties.DiscussionThe results of this study revealed differences in the structural characteristics of endophytic bacterial communities between resistant and susceptible tobacco varieties, with groups such as Paenibacillus potentially playing significant roles in resisting BWD. These findings highlight the superiority of seed endophytic microorganisms. In the context of declining plant disease resistance and the spread of bacterial wilt, core endophytic microorganisms in seeds may emerge as a viable option for enhancing the productivity of agricultural ecosystems.
Potential geographical distribution of Cordyceps cicadae and its two hosts in China under climate change
Authors: Chen Junyi , He Donglan 
Published: January 2025

IntroductionThe fungus Cordyceps cicadae is both edible and medicinal.MethodsTo acquire a thorough comprehension of its distribution in China, two host insects, Macrosemia pieli and Platypleura kaempferi, were selected as biological factors potentially associated with its distribution, the ENMTools program was utilized to ascertain the principal environmental factors affecting the distribution of potentially suitable habitats. The possible geographic distributions in the present as well as in the 2030s, 2050s, and 2070s were then predicted using the optimized MaxEnt model.ResultsThe primary environmental variables were soil pH, mean diurnal range, annual precipitation, precipitation seasonality, annual mean temperature and precipitation of the driest month. C. cicadae thrived on steep slopes. and some of which also significantly affect the distribution of its two hosts. Most of the suitable habitats of C. cicadae and M. pieli were currently found in the subtropical monsoon zone. The SSP126, SSP370, and SSP585 scenarios were associated with positive, stable, and unfavorable impacts on the extent of suitable habitats for C. cicadae, respectively, and the suitability of P. kaempferi decreased under three different conditions. The expansion of the C. cicadae was observed in provinces bordering the middle and lower reaches of the Yellow River, as well as in Zhanjiang, Guangdong Province, and northern Yunnan Province. Conversely, its habitat contraction was mainly found in western Guangdong, southern Guangxi, northern Hainan, southwestern Yunnan, and areas bordering eastern Sichuan. The shared contraction regions with its two hosts were primarily located in western Guangdong, southern Guangxi, and southern Sichuan. Moreover, the future centroids were found at higher elevations than the present ones in the provinces of Jiangxi and Hunan.DiscussionIn light of climate change, this research held significance for the conservation and sustainable utilization of C. cicadae.
Analysis of study Global Burden of Disease in 2021: global, regional, and national burden of nutritional deficiency from 1990 to 2021
Authors: Chen Jing , Li Zedong , Liu Hong 
Published: January 2025

BackgroundNutrient deficiency disorders (NDs) harm growth, causing economic losses. Addressing NDs is a global priority, yet recent data is limited. This study examines latest NDs data across 204 countries and 21 regions from 1990 to 2021.MethodsData from the 2021 Global Burden of Disease (GBD) study were used to analyze NDs-related incidence, prevalence, deaths, and disability-adjusted life years (DALYs) at global, national, and regional levels. Joinpoint regression analysis was applied to evaluate temporal trends, with Estimated Annual Percentage Change (EAPC) assessing long-term patterns.ResultsIn 2021, the global burden of NDs remained substantial, with a total of 1,845,246,558 cases with an ASPR of 23,858.99 cases per 100,000 individuals (95% UI: 23,445.77–24,320.82). The ASIR was 7,725.1 per 100,000 people (95% UI: 7,404.01–8,109.01), while the ASMR was 3.03 per 100,000 persons (95% UI: 2.69–3.4). Additionally, age-standardized DALYs rate was 657.62 per 100,000 individuals (95% UI: 489.93–869.58). Regionally, areas with low SDI exhibited the greatest ASPR, ASIR, ASDR, and age-standardized DALYs rates, whereas high SDI regions had the lowest rates.ConclusionsAlthough global NDs burden has declined from 1990 to 2021, regional and demographic disparities remain. Enhanced healthcare access in high-risk SDI regions is essential to further mitigate NDs's global impact.
Intratumor microbiome-derived butyrate promotes chemo-resistance in colorectal cancer
Authors: Xu Linsheng , Hu Bingde , He Jingli , Fu Xin , Liu Na 
Published: January 2025

IntroductionColorectal cancer (CRC) is a leading cause of cancer-related mortality globally. Although tumor immunotherapy is widely recognized for treating unresectable CRC, challenges such as ineffective immunotherapy and drug resistance remain prevalent. While intratumor microbiome-derived butyrate has been implicated in promoting lung cancer metastasis, its role in CRC chemoresistance is not well understood. This study aimed to explore the relationship between intratumor butyrate and chemoresistance in CRC.MethodsWe performed a comprehensive analysis of the microbiome composition in CRC patients with varying resistance-free survival (RFS) durations, utilizing 16S rRNA sequencing. Furthermore, we assessed the prognostic significance of circulating microbiome DNA (cmDNA) and examined the effects of exogenous butyrate supplementation on the chemosensitivity of CRC cell lines.ResultsOur 16S sequencing analysis revealed a reduction in microbial diversity within tumor samples of patients with resistance, as indicated by metrics such as observed taxonomic units, Shannon, and Simpson indices. Notably, Roseburia and Fusobacteria emerged as prominent biomarkers for the resistance group, whereas Bifidobacterium, Helicobacter, and Akkermansia were identified as biomarkers for the non-resistant group. Utilizing a Lasso regression model, we identified six genera-Roseburia, Helicobacter, Gardnerella, Flavonifractor, Coprococcus, and Anaerostipes-that significantly correlated with recurrence-free survival. Furthermore, both the intratumor microbiome signature and circulating microbiome DNA were effective in accurately predicting CRC resistance. Experimental assays, including CCK8 and wound-healing, demonstrated that intratumor microbiome-derived butyrate enhances the proliferation and migration of HCT15 cells in a time- and concentration-dependent manner. Cell survival analysis further indicated that butyrate treatment significantly increased the IC50 value, suggesting heightened drug resistance in HCT15 cells. Mechanistically, this resistance was attributed to butyrate’s activation of the PI3K-AKT signaling pathway.ConclusionOur results suggest that intratumor microbiome-derived butyrate contributes to chemoresistance in colorectal cancer, highlighting the potential prognostic and therapeutic significance of the intratumor microbiome.