bluuebunny/crossref_metadata_2025_split huggingface dataset:
Pharmaceutical development of sangviritrin spray for the treatment of inflammatory diseases of the oral cavity
Authors: Anurova M. N. , Dorokhina Ya. A. , Gulenkov A. S. , Demina N. B. , Korol L. A. , Mizina P. G. 
Published: January 2025

Introduction. One of the most common bacterial infections includes acute and chronic tonsillitis, affecting 10 to 15 % of adults and approximately 20–25 % of children worldwide, according to the World Health Organization. The focus of this study was an original substance, sangviritrin, containing no less than 96.5 % of the sum of bisulfates of two alkaloids: sanguinarine and chelerythrine. Given the high antimicrobial activity of sangviritrin, the research aimed to develop a local delivery system based on this medicinal substance.Aim. The aim of this work was to develop an oral medicinal form of sangviritrin.Materials and Methods. Sangviritrin, produced by the All-Russian Scientific Research Institute of Medicinal and Aromatic Plants, was used as the active pharmaceutical ingredient. The selection of auxiliary components was carried out in accordance with the methodologies specified in the State Pharmacopoeia XV edition. The organoleptic properties of the substance and spray samples were studied using A. I. Tenzova's method. The local irritant action of the spray was assessed using the conjunctival test method on chinchilla rabbits weighing 3.5–4.3 kg. Quantitative determination of sangviritrin in the medicinal form was performed using spectrophotometric methods at a wavelength of 321 ± 2 nm on a spectrophotometer SPECORD® 200 PLUS (Analytik Jena GmbH+Co. KG, Germany).Results and Discussion. Optimal auxiliary component compositions were selected based on measurements of spray technological parameters. A comprehensive approach was justified for correcting the organoleptic properties of the substance, involving the introduction of a minimal amount of sweeteners and additional use of β-cyclodextrin. Examination of the local irritant action indicated a "weak or absent" degree of local irritation.Conclusion. A local delivery system for sangviritrin has been developed for the treatment of infectious and inflammatory diseases of the oral cavity.
Desafíos y oportunidades del comercio electrónico para las MYPIMES, un enfoque práctico
Authors: Barragán-Quizhpe Christian , Rivadeneira-Ramos Edgar , León-Monar Patricia , Quizhpe-Baculima Víctor 
Published: January 2025

El artículo examina los desafíos y oportunidades que enfrentan las MYPIMES de Bolívar, Ecuador, en la adopción del comercio electrónico en un contexto impulsado por la pandemia de COVID-19 y la globalización. Aunque el comercio electrónico ofrece oportunidades significativas, las MYPIMES enfrentan barreras como la falta de infraestructura digital, limitaciones financieras y escasas habilidades tecnológicas. El estudio tiene como objetivo analizar estas barreras y proponer soluciones prácticas que fortalezcan su competitividad digital.
La metodología emplea un enfoque mixto, combinando revisión documental y encuestas a 334 representantes de MYPIMES en Bolívar. Este enfoque permite triangulación de datos, reforzando la validez de los resultados. Los hallazgos destacan una brecha digital considerable respecto a las grandes empresas; mientras el 78% ha participado en eventos virtuales, solo el 23% usa comercio electrónico para vender. Las dificultades clave incluyen generación de clientes, marketing y acceso a financiamiento.
La investigación concluye que la falta de competencias tecnológicas es un obstáculo relevante para expandir la presencia en línea y diversificar la oferta de productos y servicios. También se subraya que muchas MYPIMES prefieren plataformas gratuitas, lo que resalta la necesidad de reducir costos operativos. Este estudio proporciona una visión amplia de los retos y propone estrategias prácticas que faciliten la integración efectiva de las MYPIMES en el comercio digital.
Recaudación fiscal en el sector de la construcción provincia de Santa Elena: Análisis y tendencias 2019-2023
Authors: Parrales-Chalen Giovanny , Pazmiño-Enríquez José 
Published: January 2025

El sector de la construcción ha sido considerado un actor importante en el desarrollo socio-económico de la provincia de Santa Elena, Ecuador. Este estudio examina las tendencias de recaudación fiscal en este sector comprendidos en el período 2019-2023, con base en datos estadísticos oficiales. Se analizaron con profundidad los patrones de ingresos fiscales, con una determinación de factores clave que tienen un nivel de influencia en los ingresos especialmente en el periodo de la pandemia COVID 19. El objetivo del estudio fue examinar las tendencias de ingresos fiscales en el ámbito de la construcción en la provincia de Santa Elena entre 2019 y 2023, con el fin de mejorar las políticas fiscales y fomentar un crecimiento sostenible en el sector. La metodología abarca análisis descriptivos e inferenciales de datos oficiales sobre la recaudación fiscal. Los resultados muestran que hay una evidente tendencia al alza en la recaudación fiscal, mientras que la pandemia representó un grave reto para las capacidades institucionales.. Esto coadyuvara a la generación de políticas y estrategias de eficiencia en la gestión de ingresos fiscales y fomentar un crecimiento equitativo del sector de la construcción en la provincia de Santa Elena.
Hybridized triboelectric nanogenerators for simultaneously scavenging droplet and wind energies
Authors: Hu Chaosheng , Bao Chengmin , Liu Yang , Yan Yingzhan , Bai Yanan , Xu Qian 
Published: January 2025

Triboelectric nanogenerators (TENGs) have attracted great attention due to the simple manufacturing process, low cost, and diverse forms of energy harvesting. However, the energy collected by individual TENG is relatively limited, making it necessary to develop new method to enhance the energy harvesting capability of TENG. Here, we design a hybridized TENG that integrates a droplet-driven TENG and a wind-driven TENG, which exhibits excellent electrical performance. Under the simulated environment of medium rain with medium breeze, the hybridized TENG generates an output voltage of 95.10 V, a maximum average power of 18.15 μW, an energy of 181.54 μJ in 10 s, and charges a 1 μF capacitor to 43.29 V in 120 s. This work enables the harvesting of dispersed wind and droplet energy from the environment, providing new ideas and possibilities for online monitoring in remote areas and the construction of Internet of Things systems.
The potential of integrating conscious living into education for generation Z in the light of primary data
Authors: Garai-Fodor Mónika , Huszák Nikolett 
Published: January 2025

IntroductionIn this study, we investigate the cognitive aspects of conscious consumer behaviour among Generation Z members. We argue that conscious consumption can greatly help to foster social responsibility, environmental and health-conscious behaviour and ethical consumerism. We believe that it is an important educational task to promote and reinforce consumer behaviour among young people that increases and creates community value.MethodsIn this study, we analysed the dimensions of young people’s conscious consumer behaviour and its manifestation in concrete forms of behaviour, purchasing and consumer decisions. The primary research used a pre-tested standardised questionnaire online survey using a snowball sampling technique. Data were evaluated using bivariate and multivariate analyses in addition to descriptive statistics.ResultsThe results allowed us to identify exactly what conscious living means to young people, what behavioural elements they associate with it and what activities they themselves carry out in this context. The results show that conscious living among young people is most closely linked to healthy lifestyle activities and is closely related to good self-awareness.DiscussionThe results show that the vast majority of the Generation Z respondents surveyed do not have a realistic picture of themselves in terms of a conscious lifestyle, although self-awareness and self-identity can be seen as the basis of a conscious lifestyle. Therefore, we believe that there is a great need to adequately integrate conscious living into education. Authentic, targeted education campaigns for Generation Z can best help to equip young people with the right self-awareness and the right information about conscious living.
Association between the triglyceride/high-density lipoprotein (TG/HDL) ratio and incidence of gout: A nationwide cohort study
Authors: Chang Yoonkyung , Park Ju-young , Song Tae-Jin 
Published: January 2025

IntroductionThe global burden of gout, a severe and painful arthralgia, is of note and is expected to increase in the future. We aimed to investigate the association between the triglyceride/high-density lipoprotein (TG/HDL) ratio, a simple and validated biomarker for insulin resistance, and the incidence of gout in a longitudinal setting in the general population.MethodsOur study was conducted using the National Health Insurance Service-Health Screening Cohort database of Republic of Korea (2002–2019). We included 300,107 participants who had no previous history of gout and had data for more than three repeated measurements of the triglyceride-glucose (TyG) index. The incidence of gout was determined using at least two or more claims of the ICD-10 code M10.ResultsDuring a median 9.62 years (interquartile range 8.72–10.53), 14,116 individuals (4.72%) had a reported incidence of gout. In a fully adjusted multivariable time-dependent Cox proportional hazards model with repeated measures of the TyG index, a unit increase in the index significantly increased the risk of gout in the entire cohort (hazard ratio (HR) = 1.150, 95% confidence interval (CI) 1.116–1.184). In a multivariable Cox proportional model of average TyG index quartiles, comparison of the lowest (Q1) and highest quartiles (Q4) indicated a significant positive association with the incidence of gout (HR: 1.326, 95% CI: 1.260–1.397). This association was non-linear (J-shape) when assessing the entire cohort and the diabetes and non-diabetes cohorts.ConclusionOur study demonstrated that increased TyG index was associated with an incidence risk of gout in the general population. Additionally, this association was non-linear (J-shape) not only in the entire cohort, but also in diabetes mellitus and non-diabetes mellitus cohorts. The TyG index may be an important predictor of gout.
Ecological restoration in high-altitude mining areas: evaluation soil reconstruction and vegetation recovery in the Jiangcang coal mining area on the Qinghai-Tibet Plateau
Authors: Feng Shaohua , Li Zhiwei , Zhang Ce , Qi Ran , Yang Liya 
Published: January 2025

IntroductionThis study evaluates the effectiveness of soil reconstruction and restoration in the Jiangcang coal mining area on the Qinghai-Tibet Plateau, where harsh environmental conditions pose significant challenges to ecological restoration.MethodsTwo phases of ecological restoration were implemented, with outcomes assessed based on vegetation coverage, species diversity, biomass, soil properties, and community similarity.ResultsThe results demonstrate that soil reconstruction significantly improved soil fertility, vegetation coverage, and community stability without noticeable degradation over time. The use of sheep manure increased species diversity by introducing native seeds, addressing the shortage of suitable grass species in alpine areas. Comparatively, the second phase of restoration, which included soil reconstruction, has elevated the vegetation coverage to 80%, matching natural background levels, and has also demonstrated superior outcomes in terms of soil stability, nutrient content, and other aspects compared to traditional methods. While aboveground biomass showed rapid recovery, belowground biomass lagged, indicating a need for longer-term restoration. Restored slopes exhibited higher similarity to natural alpine meadows compared to platforms, suggesting the dominance of the artificially seeded species on the platform areas hinders the reproduction of other species, which is unfavorable for the evolution of vegetation diversity.DiscussionThis study emphasizes the effectiveness of soil reconstruction, organic amendment, and other restoration measures, providing important experience and reference for mine ecological restoration in similar high-altitude mining areas.
NLRP3 deficiency aggravated DNFB-induced chronic itch by enhancing type 2 immunity IL-4/TSLP-TRPA1 axis in mice
Authors: Huang Si-Ting , Chen Zuo-Ming , Peng Zhe , Wang Yu , Yang Fan , Tang Yang , Li Zi , Wan Li 
Published: January 2025

BackgroundThe nod-like receptor family pyrin domain-containing 3 (NLRP3) has been implicated in various skin diseases. However, its role in mediating 2, 4-dinitrofluorobenzene (DNFB)-induced chronic itch remains unclear.MethodsWidetype (WT) and Nlrp3 deletion (Nlrp3-/-)mice, the expression of transient receptor potential (TRP) ankyrin 1 (TRPA1) inhibitor or recombinant mice interleukin-18 (IL-18) were used to establish and evaluate the severity of DNFB-mediated chronic itch. Quantitative real-time PCR, western blotting, immunohistochemistry staining, immunofluorescence staining and enzyme-linked immunosorbent assay (ELISA) was used to examine the expression of NLRP3 inflammasome, type 2 immunity and receptors in dorsal root ganglion (DRG) neurons related with chronic itch. Flow cytometry was performed to quantify the frequency of type 2 immune cells.ResultsThis study revealed that the NLRP3 inflammasome was activated in the skin of DNFB-induced chronic itch mice. Surprisingly, the absence of Nlrp3 exacerbated itch behavior. In Nlrp3-/- mice, IL-18 expression was downregulated, whereas markers of type 2 immunity, such as IL-4 and thymic stromal lymphopoietin (TSLP), were significantly upregulated in the skin. Furthermore, TRPA1 and its colocalization with the IL-4 receptor were increased in the DRG. Inhibition of TRPA1 or administration of recombinant IL-18 significantly reduced DNFB-induced itch behavior in Nlrp3-/- mice. Recombinant IL-18 also decreased the expression of TRPA1, IL-4, and TSLP.DiscussionThese findings suggested that the absence of Nlrp3 aggravated DNFB-induced chronic itch by exacerbating type 2 immunity in the skin and enhancing the IL-4/TSLP-TRPA1 axis, potentially driven by reduced IL-18 levels.
Nationwide multi-centric prospective study for the identification of biomarkers to predict the treatment responses of nivolumab through comprehensive analyses of pretreatment plasma exosome mRNAs from head and neck cancer patients (BIONEXT study)
Authors: Sato Kuniaki , Toh Satoshi , Murakami Taku , Nakano Takafumi , Hongo Takahiro , Matsuo Mioko , Hashimoto Kazuki , Sugasawa Masashi , Yamazaki Keisuke , Ueki Yushi , Nakashima Torahiko , Uryu Hideoki , Ono Takeharu , Umeno Hirohito , Ueda Tsutomu , Kano Satoshi , Tsukahara Kiyoaki , Watanabe Akihito , Ota Ichiro , Monden Nobuya , Iwae Shigemichi , Maruo Takashi , Asada Yukinori , Hanai Nobuhiro , Sano Daisuke , Ozawa Hiroyuki , Asakage Takahiro , Fukusumi Takahito , Masuda Muneyuki 
Published: January 2025

BackgroundNivolumab paved a new way in the treatment of patients with recurrent or metastatic (RM) head and neck squamous cell carcinoma (RM-HNSCC). However, the limited rates of long-term survivors (&lt; 20%) demand a robust prognostic biomarker. This nationwide multi-centric prospective study aimed to identify a plasma exosome (PEX) mRNA signature, which serves as a companion diagnostic of nivolumab and provides a biological clue to develop effective therapies for a majority of non-survivors.MethodsPre-treatment plasmas (N = 104) of RM-HNSCC patients were subjected to comprehensive PEX mRNA analyses for prognostic marker discovery and validation. In parallel, paired treatment-naïve tumor and plasma samples (N = 20) were assayed to elucidate biological implications of the PEX mRNA signature.ResultsAssays for pre-treatment blood samples (N = 104) demonstrated that a combination of 6 candidate PEX mRNAs plus neutrophil-to-lymphocyte ratio precisely distinguished non-survivors from &gt;2-year survivors (2-year OS; 0% vs 57.7%; P = 0.000124) with a high hazard ratio of 2.878 (95% CI 1.639-5.055; P = 0.0002348). Parallel biological assays demonstrated that in the paired treatment-naïve HNSCC tumor and plasma samples (N = 20), PEX HLA-E mRNA (a non-survivor-predicting marker) was positively corelated with overexpression of HLA-E protein (P = 0.0191) and the dense population of tumor-infiltrating NK cells (P = 0.024) in the corresponding tumor, suggesting that the HLA-E-NKG2A immune checkpoint may inhibit the antitumor effect of PD-1blockade.ConclusionThe PEX mRNA signature could be useful as a companion diagnostic of nivolumab. The combination of an anti-NKG2A antibody (i.e., monalizumab) and nivolumab may serve as a treatment option for non-survivors predicted by a RT-qPCR-based pre-treatment measurement of PEX mRNAs.
Preparation and application of a Brucella multiepitope fusion protein based on bioinformatics and Tandem Mass Tag-based proteomics technology
Authors: Wu Qi , Yuan Yuan , Guo Liping , Xie Yujia , Yao Meixue , Yin Dehui 
Published: January 2025

IntroductionBrucellosis is a widespread zoonotic disease that poses a considerable challenge to global public health. Existing diagnostic methods for this condition, such as serological assays and bacterial culture, encounter difficulties due to their limited specificity and high operational complexity. Therefore, there is an urgent need for the development of enhanced diagnostic approaches for brucellosis.MethodsTandem mass tag (TMT) proteomic analysis was conducted on the wild-type strain Brucella abortus (B. abortus) DT21 and the vaccine strain B. abortus A19 to identify proteins with high expression levels. The proteins that exhibited high expression in the wild-type strain were selected based on the proteomic results. Subsequently, B-cell linear epitopes were predicted using multiple computational tools, including ABCpred, SVMTriP, BCPred, and Bepipred Linear Epitope Prediction 2.0. These epitopes were concatenated to construct a multiepitope fusion protein. Following prokaryotic expression and purification, an indirect enzyme-linked immunosorbent assay (iELISA) was developed. A total of 100 positive serum samples, 96 negative serum samples, and 40 serum samples from patients infected with other pathogens were collected and analyzed using the established iELISA. Furthermore, the protein was assessed for its capability to differentiate human brucellosis from lipopolysaccharide (LPS).ResultsProteomic analysis revealed the presence of 152 proteins with high expression levels in the wild-type strains. A multiepitope fusion protein, comprising a total of 32 predicted B-cell linear epitopes, was successfully prepared. The results from the iELISA indicated that the multiepitope fusion protein exhibited exceptional diagnostic performance, evidenced by an area under the receiver operating characteristic curve (AUC) of 0.9576, a sensitivity of 0.9300, and a specificity of 0.8542. In comparison to the commonly utilized LPS antigen, the fusion protein demonstrated a reduced level of cross-reactivity.ConclusionsA novel multiepitope fusion protein has been successfully developed utilizing bioinformatics and TMT proteomics technology. This fusion protein demonstrates significant potential as a diagnostic antigen for brucellosis, exhibiting high sensitivity and specificity.
The efficacy and safety of PD-1 inhibitors combined with chemotherapy treatment for advanced esophageal cancer: a network meta-analysis
Authors: Tian Jian-Zhou , Zhang Li , Lin Fu-Yong , He Ren-Jiao , Tian Wen-Rong , Yan Liu , Huang Guo-Xin , Ai Jin-Wei , Pei Bin , Li De-Sheng 
Published: January 2025

ObjectiveThis study systematically evaluated the efficacy of programmed death 1 (PD-1) inhibitors combined with chemotherapy for advanced esophageal cancer (EC).MethodsPubMed, Embase, Web of Science, Scopus, and Cochrane Library were searched to identify related randomized controlled trials (RCTs).ResultsSeven RCTs involving 4,363 participants were included. The results of the direct comparison showed that, compared with chemotherapy alone, PD-1 inhibitors combined with chemotherapy significantly improved overall survival (OS) (HR = 0.69, 95%CI = 0.63–0.74), progression-free survival (PFS) (HR = 0.63, 95%CI = 0.58–0.67), objective response rate (ORR) (RR = 1.41, 95%CI = 1.28–1.57), but were associated with a slight increase in treatment-related adverse events (AEs) (RR = 1.08, 95%CI = 1.03–1.14). The results of the network meta-analysis showed that toripalimab, sintilimab or camrelizumab, and nivolumab combined with chemotherapy were the best in OS, PFS, and ORR, respectively, with camrelizumab showing the lowest incidence of AEs.ConclusionThese results suggest that PD-1 inhibitors combined with chemotherapy provide superior clinical benefits over chemotherapy alone, albeit with a moderate increase in AEs. However, further verification through multi-center, high-quality RCTs with larger sample sizes is needed to confirm these findings.Systematic review registration: https://www.crd.york.ac.uk/prospero/display_record.php?ID=CRD42024627485.
Predictive model for assessing the prognosis of rhabdomyolysis patients in the intensive care unit
Authors: Xiong Yaxin , Shi Hongyu , Wang Jianpeng , Gu Quankuan , Song Yu , Kong Weilan , Lyu Jun , Zhao Mingyan , Meng Xianglin 
Published: January 2025

BackgroundRhabdomyolysis (RM) frequently gives rise to diverse complications, ultimately leading to an unfavorable prognosis for patients. Consequently, there is a pressing need for early prediction of survival rates among RM patients, yet reliable and effective predictive models are currently scarce.MethodsAll data utilized in this study were sourced from the MIMIC-IV database. A multivariable Cox regression analysis was conducted on the data, and the performance of the new model was evaluated based on the Harrell’s concordance index (C-index) and the area under the receiver operating characteristic curve (AUC). Furthermore, the clinical utility of the predictive model was assessed through decision curve analysis (DCA).ResultsA total of 725 RM patients admitted to the intensive care unit (ICU) were included in the analysis, comprising 507 patients in the training cohort and 218 patients in the testing cohort. For the development of the predictive model, 37 variables were carefully selected. Multivariable Cox regression revealed that age, phosphate max, RR mean, and SOFA score were independent predictors of survival outcomes in RM patients. In the training cohort, the AUCs of the new model for predicting 28-day, 60-day, and 90-day survival rates were 0.818 (95% CI: 0.766–0.871), 0.810 (95% CI: 0.761–0.855), and 0.819 (95% CI: 0.773–0.864), respectively. In the validation cohort, the AUCs of the new model for predicting 28-day, 60-day, and 90-day survival rates were 0.840 (95% CI: 0.772–0.900), 0.842 (95% CI: 0.780–0.899), and 0.842 (95% CI: 0.779–0.897), respectively.ConclusionThis study identified crucial demographic factors, vital signs, and laboratory parameters associated with RM patient prognosis and utilized them to develop a more accurate and convenient prognostic prediction model for assessing 28-day, 60-day, and 90-day survival rates.Implications for clinical practiceThis study specifically targets patients with RM admitted to ICU and presents a novel clinical prediction model that surpasses the conventional SOFA score. By integrating specific prognostic indicators tailored to RM, the model significantly enhances prediction accuracy, thereby enabling a more targeted and effective approach to managing RM patients.