bluuebunny/crossref_metadata_2025_split huggingface dataset:
Berberrubine protects against cisplatin-induced ototoxicity by promoting folate biosynthesis
Authors: Miao Zhuang , Chang Danyang , Du Xiaodong , Sun Changling 
Published: January 2025

ObjectiveThis research investigated the possible shielding properties of BB (Berberrubine) against the harmful auditory effects of cisplatin, preliminarily delving into the underlying mechanisms responsible for this protection.MethodsHEI-OC1 cell viability was determined using a Cell Counting Kit-8 (CCK-8). The impact of BB on cochlear hair cells was studied through in vitro cochlear explants culture. Apoptosis levels were measured through Annexin V-PI, Cleaved Caspase-3, and TUNEL staining. The level of ROS (reactive oxygen species) was measured through the application of DCFH-DA, MitoSOX, and JC-1 fluorescent dyes for staining. Immunofluorescence analysis of cochlear samples from mice was conducted to quantify the hair cell count, and concurrently, ABR (Auditory Brainstem Response) testing was utilized to evaluate auditory function. The mechanism of action of BB was explored using RNA-Seq and qRT-PCR analysis.ResultsBB significantly improved cell survival rates under cisplatin treatment, reduced levels of apoptotic markers (TUNEL, Cleaved Caspase-3, Annexin V-PI), decreased ROS and MitoSOX levels, and improved JC-1 signals in both HEI-OC1 cells and cochlear hair cells in cochlear explants culture. Animal studies demonstrated that treatment with BB enhanced the survival of cochlear hair cells, reduced hearing impairment caused by cisplatin in mice. RNA-seq and qRT-PCR analysis revealed that BB influenced the expression levels of multiple genes (Ccnd2, Reln, Pgf, Mylk3, Ppplr12c, Thbsl), by promoting folate biosynthesis for hearing protection.ConclusionOur findings suggest that BB protects against cisplatin-induced hearing damage by enhancing folate biosynthesis, decreasing intracellular ROS levels, and inhibiting apoptosis.
Urolithin A increases the natural killer activity of PBMCs in patients with prostate cancer
Authors: Rogovskii Vladimir , Murugin Vladimir V. , Vorobyev Nikolay , Popov Sergey , Sturov Nikolay , Krasheninnikov Alexey , Morozov Alexander , Prokhorova Marina 
Published: January 2025

BackgroundThe natural killer (NK) activity of peripheral blood mononuclear cells (PBMCs) is a crucial defense against the onset and spread of cancer. Studies have shown that patients with reduced NK activity are more susceptible to cancer, and NK activity tends to decrease due to cancer-induced immune suppression. Enhancing the natural cytotoxicity of PBMCs remains a significant task in cancer research.MethodsThis study investigates the potential of urolithin A, a polyphenolic metabolite produced by the gut microbiota, to enhance the natural cytotoxicity of PBMCs in prostate cancer patients and healthy subjects. We investigated the possible role of aryl hydrocarbon receptor (AhR) in this capability of urolithin A. We analyzed the ability of PBMCs preincubated with urolithin A, AhR agonist or antagonist to kill K562 (human chronic myelogenous leukemia) target cells.ResultsOur results demonstrate that urolithin A enhances the natural cytotoxicity of PBMCs in a dose-dependent manner. Specifically, at a concentration of 10 μM, urolithin A increased the NK activity of PBMCs from prostate cancer patients by an average of 23% (95% CI, 7%–38%). In addition, urolithin A modulates the level of cytokine production by PBMCs, decreasing the level of fractalkine, IL-8, and MCP-3. An AhR antagonist (CH223191, 1 μM) also increased NK activity, while an AhR agonist (β-naphthoflavone, 10 μM) did not increase NK activity and partially inhibited the urolithin A-induced enhancement.ConclusionUrolithin A increases the NK activity of PBMCs from patients with prostate cancer and healthy subjects, and the AhR may be involved in this capability of urolithin A.
Fertilizer potential of biochar and ryegrass productivity in metal-contaminated soil
Authors: Antonangelo Joao Arthur , Bigatao Souza Joao Luis , Zhang Hailin 
Published: January 2025

IntroductionResponse to fertilization with biochar in contaminated soils for forage crops lacks comprehensive understanding. This study delves into the role of biochar in enhancing soil pH and phosphorus (P) and potassium (K) availability for ryegrass (Lolium perenne) in clay and silt loam metal-contaminated soils.MethodsTwo pot experiments were conducted using switchgrass-derived biochar (SGB) and poultry litter-derived biochar (PLB) with varying biochar application rates: one without plants and the other with ryegrass.ResultsResults demonstrated a significant rise in soil pH with increasing biochar rates, particularly notable for the PLB experiment with plants, attributed to PLB’s superior buffer capacity. PLB significantly improved ryegrass productivity, evident in germination percentage, plant population, and biomass, especially at a 0.5-1% biochar application rate. However, excessive biochar application (2-4%) hindered plant growth.DiscussionPLB at 1% application sufficed to barely surpass critical P and K thresholds for optimal ryegrass production, whereas SGB fell short of meeting these thresholds, highlighting the importance of biochar feedstock selection. While biochar shows promise for metal remediation and nutrient enhancement, caution is advised against excessive application, considering potential nutrient contamination risks based on feedstock variations.
Advancing precision agriculture with deep learning enhanced SIS-YOLOv8 for Solanaceae crop monitoring
Authors: Qin Ruiqian , Wang Yiming , Liu Xiaoyan , Yu Helong 
Published: January 2025

IntroductionPotatoes and tomatoes are important Solanaceae crops that require effective disease monitoring for optimal agricultural production. Traditional disease monitoring methods rely on manual visual inspection, which is inefficient and prone to subjective bias. The application of deep learning in image recognition has led to object detection models such as YOLO (You Only Look Once), which have shown high efficiency in disease identification. However, complex climatic conditions in real agricultural environments challenge model robustness, and current mainstream models struggle with accurate recognition of the same diseases across different plant species.MethodsThis paper proposes the SIS-YOLOv8 model, which enhances adaptability to complex agricultural climates by improving the YOLOv8 network structure. The research introduces three key modules: 1) a Fusion-Inception Conv module to improve feature extraction against complex backgrounds like rain and haze; 2) a C2f-SIS module incorporating Style Randomization to enhance generalization ability for different crop diseases and extract more detailed disease features; and 3) an SPPF-IS module to boost model robustness through feature fusion. To reduce the model’s parameter size, this study employs the Dep Graph pruning method, significantly decreasing parameter volume by 19.9% and computational load while maintaining accuracy.ResultsExperimental results show that the SIS-YOLOv8 model outperforms the original YOLOv8n model in disease detection tasks for potatoes and tomatoes, with improvements of 8.2% in accuracy, 4% in recall rate, 5.9% in mAP50, and 6.3% in mAP50-95.DiscussionThrough these network structure optimizations, the SIS-YOLOv8 model demonstrates enhanced adaptability to complex agricultural environments, offering an effective solution for automatic crop disease detection. By improving model efficiency and robustness, our approach not only advances agricultural disease monitoring but also contributes to the broader adoption of AI-driven solutions for sustainable crop management in diverse climates.
How to implement pairing assistance during fighting COVID-19 in China: collaborative governance between local governments under the authoritative regulation
Authors: Wei Changwei , Xu Jiaxi , Wang Zhixiang , Wu Huangyue , Wang Juan 
Published: January 2025

BackgroundThe pairing assistance policy represents a distinctive instrument utilized by the Chinese government to address major public crises. This study examines the development of a pairing assistance policy by the Chinese Government through its central authority to foster collaborative governance among local governments in areas affected by COVID-19.MethodsThe aim of the study was to gain a clear understanding of how the policy of pairing assistance in public health emergencies is successfully implemented through the top-down application of authority. A case study design was used as a methodology to present an explanatory framework for implementing pairing assistance policies during major public crises. We focus on the operational process of pairing assistance, using the assistance provided by Jiangsu Province to Huangshi City in Hubei Province as an illustration.ResultsThis paper finds that responding to a crisis requires the guidance of a central authority and the cooperation of local governments. The process is driven by three key factors: the vertical intervention of the crisis, the inevitability of horizontal cooperation and the policy allocation and incentives of the bureaucracy. The three stages of co-operative governance based on authoritative regulation work together in a step-by-step manner to enhance the effectiveness of crisis response.ConclusionThe results of the study indicate that collaborative governance under the authoritative regulation is the main reason why provincial counterpart support mechanism plays a great role in COVID-19. This study is the first to approach the study of pairing assistance from the perspective of government authority. It broadens the research horizon of local government cooperation and provides a model for future collaboration.
In vitro ovicidal studies on egg-parasitic fungus Pochonia chlamydosporia and safety tests on mice
Authors: Ma Yuan , Lv Jinbao , Jiang Lili , Fan Zhaobin , Hao Luyao , Li Zhengyi , Ma Chengyu , Wang Rui , Luo Hongliang 
Published: January 2025

IntroductionThe control of parasites infections in livestock is an ongoing concern, with parasites developing resistance to commonly used antiparasitic drugs. The current study investigated in vitro the destructive effect of the fungus Pochonia chlamydosporia on the eggs and oocysts of several equine parasites, as well as assessing the safety of the fungus in mice.MethodsS. equinus, P. equorum, Anoplocephala spp eggs and Eimeria spp. oocysts were treated with P. chlamydosporia. The prepared preparation was also administered to mice, and the physiological indexes and lesions of major tissues and organs, as well as pathological sections of tissue, were then observed.ResultsP. chlamydosporia exhibited varying degrees of efficacy in the control of S. equinus, P. equorum, Anoplocephala spp eggs and Eimeria spp. oocysts. The acute toxicity test demonstrated that there was no death or toxicity symptom observed in the mice, with no significant difference in clinical observations, such as respiration, mental state, appetite, or feces, between the control and treated mice after the feeding of the biological preparation of P. chlamydosporia.DiscussionThese findings suggested that administration of P. chlamydosporia would be safe to use in livestock and provided a rationale for its potential clinical application, pending further analyses.
Identification and characterization of multiple novel viruses in fecal samples of cormorants
Authors: Ji Li , Zhao Ran , Pei Yifei , Sun Yijie , Sun Xiaoyi , Ji Likai , Wang Xiaochun , Liu Yuwei , Shen Quan , Yang Shixing , Wang Yan , Zhang Wen 
Published: January 2025

IntroductionCormorants, as protected wild animals by the State Forestry Administration of China, have a broad distribution across China. Previous studies have shown that they can be infected with multiple viruses in the Flaviviridae, Orthomyxoviridae, Paramyxoviridae, and Polyomaviridae families. There is limited knowledge about the other viruses that cormorants may carry and infect.MethodsIn this study, we employed viral metagenomics to identify novel viruses in the fecal samples collected from cormorants in Xiamen City, Fujian Province, China.ResultsTwo novel viruses were identified, including one novel picornavirus named Cormhepa01 and one novel avain hepevirus named CormhepaE. The genome of Cormhepa01 is 7,463 bp in length, which encodes a 2,260 aa polyprotien. Similar to other known picornaviruses, the conserved NTPase, proteinase, and polymerase motifs are presented in the 2C, 3C, and 3D proteins separately. Based on the phylogenetic analysis and amino acid sequence alignment, the CormhepaE may be assigned to a new picornavirus genus. The partial genome of CormhepaE is 6,546 bp in length. Compared with other avian hepatitis E virus strains, CormhepaE has multiple variable sites, which are distributed in motifs of the methyltransferase, helicase, and RdRp domains, separately. Based on the phylogenetic analysis, CormhepaE, together with another strain MG737712 isolated from sparrow, formed a new species of the Avihepevirus genus in the Hepeviridae family.ConclusionWe identified and characterized two novel cormorant viruses in this study. The findings of this study increase our understanding of the diversity of viruses in cormorants and provide practical viral genome information for the prevention and treatment of potential viral diseases affecting this species.
El estado depresivo en los estudiantes universitarios
Authors: Macias Mera Carlos Luis , Egas Reyes María Verónica 
Published: January 2025

El estudio tuvo como objetivo determinar la expresión del estado depresivo en los estudiantes universitarios que acuden al Departamento de Bienestar Estudiantil en el área de Psicología, de la Universidad Técnica de Manabí (UTM) en la etapa post-pandemia. Se desarrolló mediante una metodología cuantitativa, de alcance exploratorio y descriptivo, de tipo transversal, tomando de instrumento al Inventario de Depresión Rasgo-Estado (IDERE). El muestreo utilizado fue no probabilístico de tipo bola de nieve y estuvo conformado por 33 personas. Como resultado se obtuvo que los participantes de la investigación, poseen en su mayoría un porcentaje alto de estado depresivo, en mujeres del 54.54% como depresivo alto y 3.03% en estado depresivo medio, en hombres un 39.39% en estado depresivo alto y 3.03% en estado depresivo medio; además, existieron ciertas expresiones dentro de la escala de estado que fueron más destacadas dentro los parámetros o ítems evaluativos. En conclusión se encontró que la población estudiada expresa un porcentaje alto de estado depresivo, además de la existencia de un índice diferencial en el género de estudiantes más afectados, y que al tomar de referencia las expresiones positivas al estado depresivo de la escala estado del IDERE, se destaca que los participantes demuestran tener un posible apoyo emocional, desmotivación de su realidad, y preocupaciones, situación que se ve expresada en síntomas depresivos.
Estrés percibido en docentes de aulas hospitalarias
Authors: León  Abad Andrea Elizabet , Terán Espinoza Juan Daniel 
Published: January 2025

Los docentes de aulas hospitalarias se desempeñan en condiciones marcadas por una carga afectiva y demanda en ocasiones intensas, por lo que perciben un nivel de estrés, que de no ser gestionado de manera apropiada podrían verse afectados en su salud física y mental. El objetivo que se plantea este estudio es el conocer los principales aspectos en los que se manifiesta el estrés percibido por los docentes en su cotidianidad. Para llevar a cabo este proceso se consideró un diseño no experimental, con un enfoque cuantitativo, de alcance descriptivo, de corte transversal, los métodos que considera este estudio son bibliográfico, analítico-sintético y estadística descriptiva; para la evaluación se utilizó la Escala de Estrés Percibido que fue aplicada a 12 profesionales que pertenecen a la Dirección Distrital de Educación 17D05, en conclusión de acuerdo con los resultados, el 58,3% de la población se enmarca en una percepción de estrés, considerada como moderada; lo que, podría ser consecuencia de la labor y entorno en el que desenvuelven; en este sentido, se hace evidente la necesidad de gestionar un espacio que permita la descarga emocional y se brinde estrategias de afrontamiento minimizando el riesgo y mejorando la calidad de vida.
The Incidence and Outcomes of Acute Kidney Disease in Critically Ill Children
Authors: Mauroner Lillian , Kellum John A. , Levey Andrew S. , Formeck Cassandra , Fuhrman Dana Y. 
Published: January 2025

Background:
Acute kidney disease (AKD) includes abnormalities of kidney function present for <90 days. Acute kidney injury (AKI) is defined as a subset of AKD, with onset within seven days. There is scant data on the rates of AKD in children and its association with outcomes. Our primary objective was to examine the rates of AKD with and without AKI and compare major adverse events (MAKE) in children in the pediatric intensive care unit (PICU).

Methods:
This is a retrospective cohort study of patients ≤18 years old who were admitted to a quaternary care PICU between 2009 and 2016 using the high-density pediatric database. All patients included in the primary analysis had a known baseline serum creatinine. Patients who had a baseline estimated glomerular filtration rate (eGFR) <60 ml/min/1.73 m2 or a history of dialysis dependence or kidney transplant were excluded. AKI and AKD were defined by Kidney Disease: Improving Global Outcomes definitions. MAKE-90 was defined as a composite outcome of death, dialysis, or persistent kidney dysfunction 90 days after PICU admission.

Results:
Among 5,922 children included in this study, 1,199 (20.2%) had AKD, of which 1,092 (91%) had AKD with AKI and 107 (8.9%) had AKD without AKI. MAKE-90 occurred in 26% (308/1,199) of those with AKD compared to 3.6% (172/4723) without (p=<0.001). MAKE-90 occurred in 26% (279/1,092) of AKD with AKI and 27% (29/107) of AKD without AKI. After adjusting for age, sex, and illness severity, compared to patients that had no AKD, patients with AKD with AKI (aOR: 14.39, 95% CI: 11.06-18.72) and patients with AKD without AKI (aOR: 7.83, 95% CI: 4.54-13.51) had a greater odds of MAKE-90.

Conclusions:
More than a quarter of pediatric critically ill patients with AKD develop MAKE-90. Even in the absence of AKI, AKD is an independent risk factor for MAKE-90.
Revisão de literatura sobre a menopausa em mulheres com diagnóstico de endometriose pélvica
Authors: Lacerda Anna Gabriela Santana de 
Published: January 2025

A endometriose pélvica é caracterizada pela presença de focos ativos ou tecido endometrial (células glandulares e estroma) fora de sua cavidade, que acomete 10% da população feminina. Segundo, Barbieri, concentrações séricas de estradiol menores que 20pg/ml promovem atrofia das lesões endometrióticas, assim como, valores superiores a 100pg/ml ocorrem ativação das lesões. O diagnóstico padrão-ouro é o anatomo-patológico, porém, atualmente, se aceitam os métodos por imagem como a ressonância magnética ou da ultrassonografia pélvica transvaginal com preparo intestinal para o diagnóstico. O tratamento deve ser preferencialmente cirúrgico, porém, voltado para o desejo reprodutivo da paciente, onde a taxa de recidiva após 12 meses pode ocorrer em 50% dos casos. Para diminuir a recidiva, devemos realizar o tratamento complementar com hormônios combinados ou somente contendo progestagênios após a intervenção cirúrgica. Na transição menopausal, o declínio da quantidade e qualidade dos folículos é caracterizada por irregularidade do ciclo menstrual com diminuição dos níveis de estradiol. Muitos tratamentos hormonais levam a amenorreia e dificultam o diagnóstico da menopausa, mesmo na presença de sintomas vasomotores. As cirúrgias ovarianas conservadoras também podem culminar com perdas da reserva folicular. A menopausa fisiológica ocorre por volta dos 50 anos de idade entre mulheres de países desenvolvidos, sendo que, e ainda não existe uma determinação muito bem esclarecida sobre a influência da endometriose pélvica sobre a mudança dessa cronologia.
ИСТОРИЯ СОВЕТСКОЙ АРХЕОЛОГИИ В ЛИЧНОСТНОМ ИЗМЕРЕНИИ: ВЛАДИМИР ФЕДОРОВИЧ ГЕНИНГ (К 100-ЛЕТИЮ СО ДНЯ РОЖДЕНИЯ)
Authors: Мельникова Ольга Михайловна 
Published: January 2025

Статья посвящена осмыслению деятельности выдающегося советского археолога, педагога, организатора науки Владимира Фёдоровича Генинга в связи со 100-летием со дня его рождения. В работе рассмотрены основные вехи жизненного пути ученого. В соответствии с выделенными периодами научной биографии археолога - пермским, ижевским, казанским, свердловским, киевским - охарактеризованы ключевые достижения исследователя, его вклад в развитие археологии Прикамья, Урала, в формирование новых предметных областей советской археологии. Они соотнесены с ключевыми задачами, которые решались советской археологией в те или иные периоды ее развития. В заключение автор, будучи ученицей В. Ф. Генинга, предлагает свое видение вклада исследователя в развитие советской археологической науки, рассуждает о спорных моментах в его научном творчестве, а также о взглядах ученого, не прошедших с точки зрения новейшего научного знания проверку временем, но ставших значимыми фактами истории советской археологии.
The article is devoted to understanding the activities of the outstanding Soviet archaeologist, teacher, organizer of science Vladimir Fedorovich Gening in connection with the 100th anniversary of the scientist's birth. The author reflects on the importance of celebrating anniversaries for the modern scientific community. The paper presents the main milestones of the scientist's life path. In accordance with the selected periods of the archaeologist's scientific biography - Perm, Izhevsk, Kazan, Sverdlovsk, Kiev - the key achievements of the researcher, his contribution to the development of the archeology of the Kama region, the Urals, and the formation of new subject areas of Soviet archaeology are highlighted and characterized. They are correlated with the key tasks that were solved by Soviet archaeology in various periods of its development. In conclusion, the author, being a student of the scientist, offers her vision of V. F. Gening's contribution to the development of Soviet archaeological science, discusses controversial points in his scientific work, as well as the scientist's research views, which have not passed the test of time from the point of view of modern scientific knowledge, but have become significant facts in the history of Soviet archaeology.
A 0.67 A mm−2 package integrated CMOS class-D LC oscillator converter
Authors: Kakuta Atsuya , Miyaji Kousuke 
Published: January 2025

Abstract
In this paper, a CMOS class-D LC oscillator converter is proposed and fully integrated into a Cu core package EOMINTM including inductors and capacitors to achieve high current density power delivery. The proposed CMOS class-D LC oscillator cell can be realized by only using low voltage 1.2 V core transistors in a 65 nm CMOS process. On-die capacitors are adopted for flying capacitors, while input and output MLCC capacitors and two air core package trace inductors are integrated into a Cu core package. From the measurements, a maximum efficiency of 57% and maximum current density of 0.67 A mm−2 are achieved.