bluuebunny/crossref_metadata_2025_split huggingface dataset:
On strictly enforced mass conservation constraints for modelling the Rainfall‐Runoff process
Authors: Frame Jonathan M. , Kratzert Frederik , Gupta Hoshin V. , Ullrich Paul , Nearing Grey S. 
Published: March 2023

AbstractIt has been proposed that conservation laws might not be beneficial for accurate hydrological modelling due to errors in input (precipitation) and target (streamflow) data (particularly at the event time scale), and this might explain why deep learning models (which are not based on enforcing closure) can out‐perform catchment‐scale conceptual and process‐based models at predicting streamflow. We test this hypothesis with two forcing datasets that disagree in total, long‐term precipitation. We analyse the roll of strictly enforced mass conservation for matching a long‐term mass balance between precipitation input and streamflow output using physics‐informed (mass conserving) machine learning and find that: (1) enforcing closure in the rainfall‐runoff mass balance does appear to harm the overall skill of hydrological models; (2) deep learning models learn to account for spatiotemporally variable biases in data (3) however this ‘closure’ effect accounts for only a small fraction of the difference in predictive skill between deep learning and conceptual models.
Activity‐Based Protein Profiling – Finding General Solutions to Specific Problems
Authors: Cravatt Benjamin F. 
Published: March 2023

AbstractIn this retrospective/perspective, I will share thoughts on developing and applying the activity‐based protein profiling (ABPP) technology, an endeavor that has consumed much of our lab's attention over our 25+ year existence. Before doing so, I first wish to thank the colleagues who so kindly contributed to this Special Issue. I am appreciative and humbled that they were willing to share their innovative and impactful science in this format.
Ear disease in pet rabbits
Authors: Keeble Emma 
Published: March 2023

Background: Ear disease is a common presenting condition in pet rabbits in veterinary practice and can involve any or all parts of the ear – the external ear canal, the middle ear and the inner ear. Our knowledge of ear disease in this species has advanced significantly over the past 10 years, with recent and relevant publications reported in the literature. A higher incidence occurs in lop‐eared rabbits, thought to be due to their pinna anatomy (Johnson and Burn 2019).Aim of the article: This article reviews rabbit ear disease, focusing on clinical signs, diagnostics and treatment in pet rabbits. Clinical signs may vary depending on the severity, chronicity and anatomical location and range, from ear scratching and head shaking with otitis externa, to facial muscle contracture and neurological deficits with otitis media. The diagnosis of ear disease is also covered in this article, which is based on clinical signs and clinical examination findings, along with diagnostic imaging, ear cytology and culture. Treatment options for rabbits with ear disease are outlined as well; these vary depending on the clinical signs, disease location and severity. Prompt diagnosis and treatment of ear disease in pet rabbits is essential to avoid progression of disease and clinical signs. This article is a useful overview for all veterinary staff treating pet rabbits in veterinary practice.
Performing surgery in goats. Part 2: surgical techniques
Authors: Dustan Benjamin 
Published: March 2023

Background: Goats, like other ruminant species, will from time to time require surgicial interventions. This could be a caesarean section following dystocia in a doe while kidding, or the surgical removal of an neoplastic eye in an aging pet pygmy goat.Aim of the article: This article, the second in a two‐part series, outlines the common surgical techniques that may be required by the general practitioner when working with caprine patients. It aims to equip veterinary practitioners with the necessary knowledge and top tips to perform some of these techniques in goats.
Pterostilbene loaded poly(vinyl alcohol)‐gelatin cryogels as potential bioactive wound dressing material
Authors: Canatar İpek , Zenger Okan , Özdaş Sibel , Baydemir Peşint Gözde 
Published: March 2023

AbstractCryogels are support materials which are good at mimicking extracellular matrix due to their excellent hydrophilicity, biocompatibility, and macroporous structure, thus they are useful in facilitating cell activities during healing process. In this study, polyvinyl alcohol‐gelatin (PVA‐Gel) based cryogel membranes loaded with pterostilbene (trans‐3,5‐dimethoxy‐4‐hydroxystilbene; PTS) (PVA‐Gel/PTS) was synthesized as wound dressing materials. PVA‐Gel and PVA‐Gel/PTS were synthesized with the polymerization yields of 96% ± 0.23% and 98% ± 0.18%, respectively, and characterized by swelling tests, Brunauer–Emmett–Teller (BET) and scanning electron microscopy (SEM) analysis. The swelling ratios were calculated as 98.6% ± 4.93% and 102% ± 5.1%, macroporosities were determined as 85% ± 2.13% and 88% ± 2.2%, for PVA‐Gel and PVA‐Gel/PTS, respectively. It was determined that PVA‐Gel and PVA‐Gel/PTS have 17 m2/g ± 0.76 m2/g and 20 m2/g ± 0.92 m2/g surface areas, respectively. SEM studies were demonstrated that they have ~100 μm pore sizes. According to 3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyltetrazolium bromide (MTT), trypan blue exclusion and live‐dead assay results, it was observed that cell proliferation, cell number and cell viability were higher in PVA‐Gel/PTS cryogel at 24, 48, and 72 h compared to PVA‐Gel. A strong and transparent fluorescent light intensity was observed indicating higher cell population in PVA‐Gel/PTS in comparison with PVA‐Gel, according to 4′,6‐diamidino‐2‐phenylindole (DAPI) staining. SEM, F‐Actin, Giemsa staining and inverted‐phase microscope image of fibroblasts in PVA‐Gel/PTS cryogels revealed that dense fibroblast proliferation and spindle‐shaped morphology of cells were preserved. Moreover, DNA agarose gel data demonstrated that PVA‐Gel/PTS cryogels had no effect on DNA integrity. Consequently, produced PVA‐Gel/PTS cryogel can be used as wound dressing material to promote wound therapies, inducing cell viability and proliferation.
Toxicity evaluation of choline ionic liquid‐based nanocarriers of pharmaceutical agents for lung treatment
Authors: Niesyto Katarzyna , Skonieczna Magdalena , Adamiec‐Organiściok Małgorzata , Neugebauer Dorota 
Published: March 2023

AbstractIn vitro cytotoxicity evaluation of linear copolymer (LC) containing choline ionic liquid units and its conjugates with an antibacterial drug in anionic form, that is, p‐aminosalicylate (LC_PAS), clavulanate (LC_CLV), or piperacillin (LC_PIP) was carried out. These systems were tested against normal: human bronchial epithelial cells (BEAS‐2B), and cancers: adenocarcinoma human alveolar basal epithelial cells (A549), and human non‐small cell lung carcinoma cell line (H1299). Cells viability, after linear copolymer LC and their conjugates addition for 72 h, was measured at concentration range of 3.125–100 μg/mL. The MTT test allowed the designation of IC50 index, which was higher for BEAS‐2B, and significantly lower in the case of cancer cell lines. The cytometric analyzes, that is, Annexin‐V FITC apoptosis assay and cell cycle analysis as well as gene expression measurements for interleukins IL6 and IL8 were carried out, and showed pro‐inflammatory activity of tested compounds toward cancer cells, while it was not observed against normal cell line.
Measuring and preliminary modeling of drift interception by plant species
Authors: Dunne Jonnie B. , Grant Shanique L. , Golus Jeffrey A. , Perine Jeffrey W. , Wolf Stefan , Winchell Michael , Ledson T. Mark , Whenham Ian 
Published: March 2023

AbstractCurrently, the concept of plant capture efficiency is not quantitatively considered in the evaluation of off‐target drift for the purposes of pesticide risk assessment in the United States. For on‐target pesticide applications, canopy capture efficiency is managed by optimizing formulations or tank‐mixing with adjuvants to maximize retention of spray droplets. These efforts take into consideration the fact that plant species have diverse morphology and surface characteristics, and as such will retain varying levels of applied pesticides. This work aims to combine plant surface wettability potential, spray droplet characteristics, and plant morphology into describing the plant capture efficiency of drifted spray droplets. In this study, we used wind tunnel experiments and individual plants grown to 10–20 cm to show that at two downwind distances and with two distinct nozzles capture efficiency for sunflower (Helianthus annuus L.), lettuce (Lactuca sativa L.), and tomato (Solanum lycopersicum L.) is consistently higher than rice (Oryza sativa L.), peas (Pisum sativum L). and onions (Allium cepa L.), with carrots (Daucus carota L.) showing high variability and falling between the two groups. We also present a novel method for three‐dimensional modeling of plants from photogrammetric scanning and use the results in the first known computational fluid dynamics simulations of drift capture efficiency on plants. The mean simulated drift capture efficiency rates were within the same order of magnitude of the mean observed rates of sunflower and lettuce, and differed by one to two orders for rice and onion. We identify simulating the effects of surface roughness on droplet behavior, and the effects of wind flow on plant movement as potential model improvements requiring further species‐specific data collection.
Risk factors for postoperative cholangitis after pancreaticoduodenectomy and evaluation of internal stenting on hepaticojejunostomy: A single‐center propensity score‐based analysis
Authors: Sakai Hiroki , Notake Tsuyoshi , Shimizu Akira , Kubota Koji , Masuo Hitoshi , Yoshizawa Takahiro , Hosoda Kiyotaka , Hayashi Hikaru , Yasukawa Koya , Soejima Yuji 
Published: March 2023

AbstractBackground/PurposeThis retrospective study aimed to investigate the risk factors for postoperative cholangitis (POC) after pancreaticoduodenectomy (PD) and the efficacy of stenting on hepaticojejunostomy (HJ).MethodsWe investigated 162 patients. Postoperative cholangitis occurring before and after discharge was defined as early‐onset POC (E‐POC) and late‐onset POC (L‐POC), respectively. Risk factors for E‐POC and L‐POC were identified using univariate and multivariate logistic regression analyses. Propensity score matching (PSM) between the stenting group (group S) and the non‐stenting group (group NS), and subgroup analysis in patients with risk factors were performed to evaluate the efficacy of stenting on HJ in preventing POC.ResultsBody mass index (BMI) ≥ 25 kg/m2 and preoperative non‐biliary drainage (BD) were risk factors for E‐POC and L‐POC, respectively. PSM analysis revealed that E‐POC occurrence was significantly higher in group S than in group NS (P = .045). In the preoperative non‐BD group (n = 69), E‐POC occurrence was significantly higher in group S than in group NS (P = .025).ConclusionsBMI ≥ 25 kg/m2 and preoperative non‐BD status were risk factors for E‐POC and L‐POC, respectively. Stenting on HJ implants did not prevent POC after PD.
Unbalance between working memory task‐activation and task‐deactivation networks in epilepsy: Simultaneous EEG‐fMRI study
Authors: Qin Yun , Jiang Sisi , Xiong Siwei , Li Sipei , Fu Qiankun , Yang Lili , Du Peishan , Luo Cheng , Yao Dezhong 
Published: March 2023

AbstractWorking memory (WM) is a cognitive function involving emergent properties of theta oscillations and large‐scale network interactions. The synchronization of WM task‐related networks in the brain enhanced WM performance. However, how these networks regulate WM processing is not well known, and the alteration of the interaction among these networks may play an important role in patients with cognitive dysfunction. In this study, we used simultaneous EEG‐fMRI to examine the features of theta oscillations and the functional interactions among activation/deactivation networks during the n‐back WM task in patients with idiopathic generalized epilepsy (IGE). The results showed that there was more enhancement of frontal theta power along with WM load increase in IGE, and the theta power was positively correlated with the accuracy of the WM tasks. Moreover, fMRI activations/deactivations correlated with n‐back tasks were estimated, and we found that the IGE group had increased and widespread activations in high‐load WM tasks, including the frontoparietal activation network and task‐related deactivation areas, such as the default mode network and primary visual and auditory networks. In addition, the network connectivity results demonstrated decreased counteraction between the activation network and deactivation network, and the counteraction was correlated with the higher theta power in IGE. These results indicated the important role of the interactions between activation and deactivation networks during the WM process, and the unbalance among them may indicate the pathophysiological mechanism of cognitive dysfunction in generalized epilepsy.
Lactobacillus plantarumHF02 alleviates lipid accumulation and intestinal microbiota dysbiosis in high‐fat diet‐induced obese mice
Authors: Chen Haoran , Zhao Haiding , Qi Xiaofen , Sun Yue , Ma Ying , Li Qiming 
Published: March 2023

AbstractBACKGROUNDObesity is closely associated with lipid accumulation and intestinal microbiota dysbiosis. It has been proved that probiotics supplement contributes to alleviate obesity. The objective of this study was to investigate the mechanism by which Lactobacillus plantarum HF02 (LP‐HF02) alleviated lipid accumulation and intestinal microbiota dysbiosis in high‐fat diet‐induced obese mice.RESULTSOur results showed that LP‐HF02 ameliorated body weight, dyslipidemia, liver lipid accumulation, and liver injury in obese mice. As expected, LP‐HF02 inhibited pancreatic lipase activity in small intestinal contents and increased fecal triglyceride levels, thereby reducing dietary fat hydrolysis and absorption. Moreover, LP‐HF02 ameliorated the intestinal microbiota composition, as evidenced by the enhanced ratio of Bacteroides to Firmicutes, the decreased abundance of pathogenic bacteria (including Bacteroides, Alistipes, Blautia, and Colidextribacter) and the increased abundance of beneficial bacteria (including Muribaculaceae, Akkermansia, Faecalibaculum, and Rikenellaceae_RC9_gut_group). LP‐HF02 also increased fecal short‐chain fatty acids (SCFAs) levels and colonic mucosal thickness, and subsequently decreased serum lipopolysaccharide (LPS), interleukin‐1β (IL‐1β), and tumor necrosis factor‐α (TNF‐α) levels in obese mice. Additionally, reverse transcription quantitative polymerase chain reaction (RT‐qPCR) and Western blot results demonstrated that LP‐HF02 ameliorated hepatic lipid accumulation via activating the adenosine monophosphate (AMP)‐activated protein kinase (AMPK) pathway.CONCLUSIONTherefore, our results indicated that LP‐HF02 could be considered as a probiotic preparation for preventing obesity. © 2023 Society of Chemical Industry.
Nutritional significance, value‐added applications, and consumer perceptions of food legumes: A review
Authors: Affrifah Nicole S. , Uebersax Mark A. , Amin Samir 
Published: March 2023

AbstractLegume crops are widely grown worldwide and a primary source of proteins across many least developed countries. These food crops are inherently produced in environmentally sustainable manner and are also an economical source of plant‐based proteins versus animal‐based proteins. The nutrient composition of legumes is very rich, that is, high content of protein, fiber, and bioactive compounds and relatively lower carbohydrates than cereal crops. Although food legumes are a staple in more than 70 countries mostly in Asian, African, and South American regions, the per capita consumption in the Western countries continues to be low in spite of legumes' demonstrated health benefits. However, emerging consumer trends and preferences toward heathy foods, animal protein alternatives, and environmental concerns can enhance the consumption of legume‐based foods. The functional attributes of legume ingredients (e.g., thickening, water‐holding index, gelation, emulsification, and foaming capacity) make legumes suitable for replacing proteins from other sources. The development and marketing of legume‐based ingredients for use in various value‐added food applications have been increasing. The value‐added use of pulse protein isolates and concentrates is expanding fast with substantial use as meat extenders/replacers and meat analogs, milk substitutes, and gluten‐free applications. To increase the utilization of legumes, including legume‐based foods, it is important to focus on research and development efforts that promote “easy‐to‐cook”/prepare foods for consumers, who often avoid legumes due to long cooking times. Addressing this aspect of convenience is particularly critical in developed countries due to the busy lifestyles of consumers and potentially increase legume consumption.
Real‐world burden and treatment of chronic rhinosinusitis in Japan: A retrospective claims database analysis
Authors: Yoshikawa Mamoru , Sunaga Yoshinori , Koshiba Ryuji , Inukai Miho , Takeuchi Makiko 
Published: March 2023

AbstractObjectivesChronic rhinosinusitis (CRS) is a heterogeneous disease, which can be subdivided into CRS with (CRSwNP) or without (CRSsNP) nasal polyps. An intractable form of CRSwNP that is associated with an eosinophil‐dominant inflammatory cell infiltration (eosinophilic CRS) has become more prevalent in Japan. There is currently limited information on the burden of CRS in Japan and treatment approaches used in real‐world practice.MethodsThis retrospective, observational, comparative cohort study used information from the Japanese JMDC insurance claims database (study period April 1, 2015, to March 31, 2020). A CRS cohort was identified and matched with a control group without CRS. The primary objective was to clarify disease burden and treatment approaches by comparing comorbidities, healthcare resource utilization (HRU), and drug prescriptions in the CRS and non‐CRS groups.ResultsIn total, 23,256 individuals with CRS (1762 with CRSwNP and 21,494 with CRSsNP) were matched with 23,256 controls. The mean age was 45 years and the majority of individuals were male (57%). Individuals with CRS had a higher disease burden than controls, with more frequent comorbidities (particularly, type 2 inflammatory disease [e.g., allergic rhinitis and asthma], and those caused by systemic corticosteroids [SCS]), and higher HRU (including outpatient visits, laboratory examinations and surgical procedures). Further, individuals with CRS were prescribed more medications, both for CRS (including SCS) and non‐CRS conditions, than controls.ConclusionIn Japan, CRS is associated with a high disease burden, and multiple treatment approaches are used in affected individuals, including long‐term SCS, which is generally not recommended.Level of Evidence3
Structure–Property Relationships in Epoxyurethane Polymers Based on Erythritol Dicarbonate
Authors: Guo Xin , Fang Xinxin , Fan Songhong , Gao Junfei , Gu Qun 
Published: March 2023

AbstractThe highly reactive erythritol dicarbonate (EDC) allows facile design and preparation of solvent‐ and catalyst‐free epoxyurethane polymers. Diglycidyl ether of bisphenol‐A (DGEBA) and oligo polyhydroxyurethanes are used to produce epoxyurethane polymers (o‐PHUs). The o‐PHUs are prepared using cyclic carbonates derived from erythritol and various diamines (aliphatic amine and polyether amine) or polyamines (diethylene triamine (DETA) and triethylne tetramine (TETA)). Active secondary amino groups derived from o‐PHUs polyamines form hydrogen bonds between urethane carbonyls in epoxy hybrid networks with the highest cross‐linking density (1293 mol m−3), glass transition temperature Tg (93 °C), and ultimate tensile strength (34 MPa). And the ether bond provides a significant potential for hydrogen bonding between hydroxyl group and ether oxygen, forming strong hydrogen bond cross‐linking between molecular chains, with cross‐linking density and ultimate tensile strength comparable to polyepoxyurethane derived from polyamines, except for a lower Tg (27 °C). In addition, these polyepoxyurethanes have superior adhesion to wood, aluminum alloy, and polymethyl methacrylate (PMMA) substrates. This versatile oligo PHUs would provide a wide range of fabrication options for adhesive epoxyurethane polymers.
Chromosome Microarray Analysis for the Investigation of Deletions in Pediatric Movement Disorders: A Systematic Review of the Literature
Authors: Soliani Luca , Alcalá San Martín Adrián , Balsells Sol , Hernando‐Davalillo Cristina , Ortigoza‐Escobar Juan Darío 
Published: March 2023

AbstractBackgroundChromosome microarray analysis (CMA) can detect copy number variants (CNV) beyond the resolution of standard G‐banded karyotyping. De novo or inherited microdeletions may cause autosomal dominant movement disorders.ObjectivesThe purpose of this study was to analyze the clinical characteristics, associated features, and genetic information of children with deletions in known genes that cause movement disorders and to make recommendations regarding the diagnostic application of CMA.MethodsClinical cases published in English were identified in scientific databases (PubMed, ClinVar, and DECIPHER) from January 1998 to July 2019 following Preferred Reporting Items for Systematic Reviews and Meta‐Analyses guidelines. Cases with deletions or microdeletions greater than 300 kb were selected. Information collected included age, sex, movement disorders, associated features, and the size and location of the deletion. Duplications or microduplications were not included.ResultsA total of 18.097 records were reviewed, and 171 individuals were identified. Ataxia (30.4%), stereotypies (23.9%), and dystonia (21%) were the most common movement disorders. A total of 16% of the patients demonstrated more than one movement disorder. The most common associated features were intellectual disability or developmental delay (78.9%) and facial dysmorphism (57.8%). The majority (77.7%) of microdeletions were smaller than 5 Mb. We find no correlation between movement disorders, their associated features, and the size of microdeletions.ConclusionsOur results support the use of CMA as an investigational test in children with movement disorders. As the majority of identified articles were case reports and small case series (low quality), future efforts should focus on larger prospective studies to examine the causation of microdeletions in pediatric movement disorders.