bluuebunny/crossref_metadata_2025_split huggingface dataset:
Metastable CrMnNi steels processed by laser powder bed fusion: experimental assessment of elementary mechanisms contributing to microstructure, properties and residual stress
Authors: Richter J. , Bartzsch G. , Scherbring S. , Bolender A. , Vollmer M. , Mola J. , Volkova O. , Niendorf T. 
Published: December 2022

AbstractThe complex thermal history imposed by the laser-based powder bed fusion of metals (PBF-LB/M) process is known to promote the evolution of unique microstructures. In the present study, metastable CrMnNi steels with different nickel contents and, thus, different phase stabilities are manufactured by PBF-LB/M. Results clearly reveal that an adequate choice of materials will allow to tailor mechanical properties as well as residual stress states in the as-built material to eventually redundantize any thermal post-treatment. The chemical differences lead to different phase constitutions in as-built conditions and, thus, affect microstructure evolution and elementary deformation mechanisms upon deformation, i.e., twinning and martensitic transformation. Such alloys designed for additive manufacturing (AM) highlight the possibility to tackle well-known challenges in AM such as limited damage tolerance, porosity and detrimental residual stress states without conducting any post treatments, e.g., stress relieve and hot isostatic pressing. From the perspective of robust design of AM components, indeed it seems to be a very effective approach to adapt the material to the process characteristics of AM.
Cataract progression following lens-sparing pars plana vitrectomy for rhegmatogenous retinal detachment
Authors: Bellucci Carlo , Benatti Lucia , Rossi Maurizio , Tedesco Salvatore Antonio , Carta Arturo , Calzetti Giacomo , Gandolfi Stefano , Mora Paolo 
Published: December 2022

AbstractLens-sparing pars plana vitrectomy (PPV) is often followed by cataract development. However, there have been few prospective studies evaluating the timing of cataract progression and potential associated factors. This was an observational study conducted at the Ophthalmology Unit of the University Hospital of Parma (Parma, Italy). Patients presenting with rhegmatogenous retinal detachment (RRD), who underwent PPV with preservation of the lens, were examined according to a scheduled follow-up (3, 6 and 12 months after PPV) and then preoperatively when cataract extraction surgery (CES) was indicated, or at the end of the study follow-up period (May 2021). The primary outcome was the interval between PPV and CES indication (based on predefined refractive criteria). A total of 36 eyes of 36 patients (mean age: 52 ± 10 years) were included in the study. Nineteen eyes (53%) were indicated for CES a median of 14.5 months (IQR: 12.0–24.8) after PPV. The nuclear and posterior subcapsular forms of cataract progressed significantly starting at 6 months after PPV. Older age at the time of PPV, silicone oil tamponade and RRD without macular involvement were significantly and independently associated with an earlier indication for CES. Patient age and the use of silicone oil tamponade must be taken into consideration when evaluating the risk of cataract development after PPV.
Ventral premotor cortex encodes task relevant features during eye and head movements
Authors: Smalianchuk Ivan , Gandhi Neeraj J. 
Published: December 2022

AbstractVisual exploration of the environment is achieved through gaze shifts or coordinated movements of the eyes and the head. The kinematics and contributions of each component can be decoupled to fit the context of the required behavior, such as redirecting the visual axis without moving the head or rotating the head without changing the line of sight. A neural controller of these effectors, therefore, must show code relating to multiple muscle groups, and it must also differentiate its code based on context. In this study we tested whether the ventral premotor cortex (PMv) in monkey exhibits a population code relating to various features of eye and head movements. We constructed three different behavioral tasks or contexts, each with four variables to explore whether PMv modulates its activity in accordance with these factors. We found that task related population code in PMv differentiates between all task related features and conclude that PMv carries information about task relevant features during eye and head movements. Furthermore, this code represents both lower-level (effector and movement direction) and higher-level (context) information.
Establishment and validation of a redox-related long non-coding RNAs prognostic signature in head and neck squamous cell carcinoma
Authors: Liu Kaitai , Huang Tianyi , Zhang Hui , Deng Hongxia , Tang Ming 
Published: December 2022

AbstractReduction and oxidation (redox) reactions occur in living organisms as part of normal cellular metabolism. Here, we established a novel redox-related long non-coding RNAs (rrlncRNAs) signature to predict the prognosis and therapeutic response in Head and neck squamous cell carcinoma (HNSCC). The expression profile and clinical information were obtained from the TCGA project. In total, 10 differently expressed rrlncRNAs associated with prognosis were identified and involved in a prognostic risk score signature by the least absolute shrinkage and selection operator penalized Cox analysis. The area under the receiver operating characteristic curves of the survival rates predicted by the rrlncRNAs signature over one, two, and three years were found to be 0.651, 0.670, and 0.679. Following the completion of the Kaplan–Meier survival study, we discovered that the lower-risk cohort exhibited a much longer overall survival period in contrast with the higher-risk cohort. Univariate and multivariable Cox regression analyses demonstrated that the risk score independently served as a significant predictive factor. GO annotation and KEGG pathway analyses illustrated that the rrlncRNAs signature was strongly associated with immune-related functions as well as signaling pathways. The tumor-infiltrating immune cells, tumor microenvironment, immune-related functions, HLA gene family expression, immune checkpoint genes expression, and somatic variants differed substantially between the low- and high-risk cohorts. Moreover, patients in low-risk group were predicted to present a favorable immunotherapy responsiveness, while in contrast, the high-risk group patients might have a stronger sensitivity to “docetaxel”. According to our findings, the rrlncRNAs signature showed an excellent prognosis predictive value and might indicate therapeutic response to immunotherapy in HNSCC.
Sensitivity of transbronchial lung cryobiopsy in the diagnosis of different interstitial lung diseases
Authors: Zaizen Yoshiaki , Tachibana Yuri , Ozasa Mutsumi , Yamano Yasuhiko , Takei Reoto , Kohashi Yasuo , Kataoka Kensuke , Saito Yuji , Tabata Kazuhiro , Okamoto Masaki , Tominaga Masaki , Fujimoto Kiminori , Sakamoto Noriho , Ashizawa Kazuto , Mukae Hiroshi , Bychkov Andrey , Johkoh Takeshi , Hoshino Tomoaki , Kondoh Yasuhiro , Fukuoka Junya 
Published: December 2022

AbstractThe accuracy of transbronchial lung cryobiopsy (TBLC) in each disease for pathological and multidisciplinary discussion (MDD) diagnosis is not yet established. Method: We investigated 431 patients who were classified by MDD diagnosis and were grouped into the disease categories. For each category or disease, we used TBLC samples to calculate the sensitivities of the pathological diagnosis compared with MDD diagnoses. Further, we compared these sensitivities to pathological diagnoses with all clinical/radiological information. Result: The sensitivity for diagnosing idiopathic interstitial pneumonia (IIPs) with TBLC was higher than connective tissue disease associated ILD (CTD-ILD). Idiopathic nonspecific interstitial pneumonia (iNSIP), fibrotic hypersensitivity pneumonitis, and some CTD-ILDs were diagnosed with lower sensitivities compared to IPF. The sensitivity of pathological diagnosis with all clinical/radiological information in IPF was higher than in iNSIP, but not significantly different from other diseases. The overall sensitivity of the pathological diagnosis with clinical/radiological information was 69.0%, significantly higher than without clinical/radiological information. Conclusion: The sensitivity of pathological diagnosis with TBLC was low for some diseases except IPF. The addition of all clinical/radiological information increased the sensitivity of pathology diagnosis by TBLC, which was no less sensitive than IPF for all diseases except iNSIP.
Analysis of the oral microbiome during hormonal cycle and its alterations in menopausal women: the “AMICA” project
Authors: Tramice A. , Paris D. , Manca A. , Guevara Agudelo F. A. , Petrosino S. , Siracusa L. , Carbone M. , Melck D. , Raymond F. , Piscitelli F. 
Published: December 2022

AbstractThe maintenance of human health is dependent on a symbiotic relationship between humans and associated bacteria. The diversity and abundance of each habitat’s signature microbes vary widely among body areas and among them the oral microbiome plays a key role. Significant changes in the oral cavity, predominantly at salivary and periodontal level, have been associated with changes in estrogen levels. However, whether the oral microbiome is affected by hormonal level alterations is understudied. Hence the main objective pursued by AMICA project was to characterize the oral microbiome (saliva) in healthy women through: profiling studies using "omics" technologies (NMR-based metabolomics, targeted lipidomics by LC–MS, metagenomics by NGS); SinglePlex ELISA assays; glycosidase activity analyses and bioinformatic analysis. For this purpose, thirty-nine medically healthy women aged 26–77 years (19 with menstrual cycle and 20 in menopause) were recruited. Participants completed questionnaires assessing detailed medical and medication history and demographic characteristics. Plasmatic and salivary levels of sexual hormones were assessed (FSH, estradiol, LH and progesteron) at day 3 and 14 for women with menstrual cycle and only once for women in menopause. Salivary microbiome composition was assessed through meta-taxonomic 16S sequencing and overall, the salivary microbiome of most women remained relatively stable throughout the menstrual cycle and in menopause. Targeted lipidomics and untargeted metabolomics profiling were assessed through the use of LC–MS and NMR spectroscopy technologies, respectively and significant changes in terms of metabolites were identified in saliva of post-menopausal women in comparison to cycle. Moreover, glycosyl hydrolase activities were screened and showed that the β-D-hexosaminidase activity was the most present among those analyzed. Although this study has not identified significant alterations in the composition of the oral microbiome, multiomics analysis have revealed a strong correlation between 2-AG and α-mannosidase. In conclusion, the use of a multidisciplinary approach to investigate the oral microbiome of healthy women provided some indication about microbiome-derived predictive biomarkers that could be used in the future for developing new strategies to help to re-establish the correct hormonal balance in post-menopausal women.
Aberrant glial activation and synaptic defects in CaMKIIα-iCre and nestin-Cre transgenic mouse models
Authors: Alia Alia O. , Jeon Sohee , Popovic Jelena , Salvo Miranda A. , Sadleir Katherine R. , Vassar Robert , Cuddy Leah K. 
Published: December 2022

AbstractCurrent scientific research is driven by the ability to manipulate gene expression by utilizing the Cre/loxP system in transgenic mouse models. However, artifacts in Cre-driver mouse lines that introduce undesired effects and confound results are increasingly being reported. Here, we show aberrant neuroinflammation and synaptic changes in two widely used Cre-driver mouse models. Neuroinflammation in CaMKIIα-iCre mice was characterized by the activation and proliferation of microglia and astrocytes in synaptic layers of the hippocampus. Increased GFAP and Iba1 levels were observed in hippocampal brain regions of 4-, 8- and 22-month-old CaMKIIα-iCre mice compared to WT littermates. Synaptic changes in NMDAR, AMPAR, PSD95 and phosphorylated CaMKIIα became apparent in 8-month-old CaMKIIα-iCre mice but were not observed in 4-month-old CaMKIIα-iCre mice. Synaptophysin and synaptoporin were unchanged in CaMKIIα-iCre compared to WT mice, suggesting that synaptic alterations may occur in excitatory postsynaptic regions in which iCre is predominantly expressed. Finally, hippocampal volume was reduced in 22-month-old CaMKIIα-iCre mice compared to WT mice. We tested the brains of mice of additional common Cre-driver mouse models for neuroinflammation; the nestin-Cre mouse model showed synaptic changes and astrocytosis marked by increased GFAP+ astrocytes in cortical and hippocampal regions, while the original CaMKIIα-Cre T29-1 strain was comparable to WT mice. The mechanisms underlying abnormal neuroinflammation in nestin-Cre and CaMKIIα-iCre are unknown but may be associated with high levels of Cre expression. Our findings are critical to the scientific community and demonstrate that the correct Cre-driver controls must be included in all studies using these mice.
The potential of multistress tolerant yeast, Saccharomycodes ludwigii, for second-generation bioethanol production
Authors: Pilap Warayutt , Thanonkeo Sudarat , Klanrit Preekamol , Thanonkeo Pornthap 
Published: December 2022

AbstractEthanol production at high temperatures using lignocellulosic biomass as feedstock requires a highly efficient thermo and lignocellulosic inhibitor-tolerant ethanologenic yeast. In this study, sixty-three yeast isolates were obtained from tropical acidic fruits using a selective acidified medium containing 80 mM glacial acetic acid. Twenty-nine of the yeast isolates exhibited significant thermo and acetic acid-tolerant fermentative abilities. All these isolates were classified into three major yeast species, namely Saccharomycodes ludwigii, Pichia kudriavzevii, and P. manshurica, based on molecular identification. Saccharomycodes ludwigii APRE2 displayed an ability to grow at high temperatures of up to 43 °C and exhibited significant multistress tolerance toward acetic acid, furfural, 5-hydroxymethyl furfural (5-HMF), and ethanol among the isolated yeast species. It can produce a maximum ethanol concentration of 63.07 g/L and productivity of 1.31 g/L.h in yeast extract malt extract (YM) medium containing 160 g/L glucose and supplemented with 80 mM acetic acid and 15 mM furfural as a cocktail inhibitor. When an acid-pretreated pineapple waste hydrolysate (PWH) containing approximately 106 g/L total sugars, 131 mM acetic acid, and 3.95 mM furfural was used as a feedstock, 38.02 g/L and 1.58 g/L.h of ethanol concentration and productivity, respectively, were achieved. Based on the results of the current study, the new thermo and acetic acid-tolerant yeast S. ludwigii APRE2 exhibited excellent potential for second-generation bioethanol production at high temperatures.
FANCD2 promotes mitotic rescue from transcription-mediated replication stress in SETX-deficient cancer cells
Authors: Said Maha , Barra Viviana , Balzano Elisa , Talhaoui Ibtissam , Pelliccia Franca , Giunta Simona , Naim Valeria 
Published: December 2022

AbstractReplication stress (RS) is a leading cause of genome instability and cancer development. A substantial source of endogenous RS originates from the encounter between the transcription and replication machineries operating on the same DNA template. This occurs predominantly under specific contexts, such as oncogene activation, metabolic stress, or a deficiency in proteins that specifically act to prevent or resolve those transcription-replication conflicts (TRCs). One such protein is Senataxin (SETX), an RNA:DNA helicase involved in resolution of TRCs and R-loops. Here we identify a synthetic lethal interaction between SETX and proteins of the Fanconi anemia (FA) pathway. Depletion of SETX induces spontaneous under-replication and chromosome fragility due to active transcription and R-loops that persist in mitosis. These fragile loci are targeted by the Fanconi anemia protein, FANCD2, to facilitate the resolution of under-replicated DNA, thus preventing chromosome mis-segregation and allowing cells to proliferate. Mechanistically, we show that FANCD2 promotes mitotic DNA synthesis that is dependent on XPF and MUS81 endonucleases. Importantly, co-depleting FANCD2 together with SETX impairs cancer cell proliferation, without significantly affecting non-cancerous cells. Therefore, we uncovered a synthetic lethality between SETX and FA proteins for tolerance of transcription-mediated RS that may be exploited for cancer therapy.
Reproducibility in Small-
N
Treatment Research: A Tutorial Using Examples From Aphasiology
Authors: Cavanaugh Robert , Quique Yina M. , Swiderski Alexander M. , Kallhoff Lydia , Terhorst Lauren , Wambaugh Julie , Hula William D. , Evans William S. 
Published: December 2022

Purpose:

Small-
N
studies are the dominant study design supporting evidence-based interventions in communication science and disorders, including treatments for aphasia and related disorders. However, there is little guidance for conducting reproducible analyses or selecting appropriate effect sizes in small-
N
studies, which has implications for scientific review, rigor, and replication. This tutorial aims to (a) demonstrate how to conduct reproducible analyses using effect sizes common to research in aphasia and related disorders and (b) provide a conceptual discussion to improve the reader's understanding of these effect sizes.

Method:

We provide a tutorial on reproducible analyses of small-
N
designs in the statistical programming language R using published data from Wambaugh et al. (2017). In addition, we discuss the strengths, weaknesses, reporting requirements, and impact of experimental design decisions on effect sizes common to this body of research.

Results:
Reproducible code demonstrates implementation and comparison of within-case standardized mean difference, proportion of maximal gain, tau-U, and frequentist and Bayesian mixed-effects models. Data, code, and an interactive web application are available as a resource for researchers, clinicians, and students.

Conclusions:

Pursuing reproducible research is key to promoting transparency in small-
N
treatment research. Researchers and clinicians must understand the properties of common effect size measures to make informed decisions in order to select ideal effect size measures and act as informed consumers of small-
N
studies. Together, a commitment to reproducibility and a keen understanding of effect sizes can improve the scientific rigor and synthesis of the evidence supporting clinical services in aphasiology and in communication sciences and disorders more broadly.

Supplemental Material and Open Science Form:

https://doi.org/10.23641/asha.21699476
Secure practical tracking of an uncertain robotic system under coarse conditions on reference trajectory and obstacles
Authors: Li Jian , Xu Wenqing , Wu Zhaojing 
Published: December 2022

AbstractThis article investigates practical tracking joint with obstacle‐avoidance for a class of uncertain robotic systems. The remarkable features are revealed from two aspects. First, more serious system uncertainties are allowed than the related literature due to the presence of unknown system matrices and mismatched disturbance in the investigated system. Second, only coarse conditions are required on reference trajectory and obstacles since their time derivatives are not necessarily to be available for feedback but those of the related literature muse be, while the reference trajectory is only required to be first rather than second order continuously differentiable. For this, a secure adaptive control framework is established by incorporating adaptive dynamic compensation mechanism into the backstepping procedure with the smart choice of adaptive law and the skillful injection of a barrier function. Consequently, a state‐feedback controller is explicitly designed which guarantees the obstacle‐avoidance of the robotic system while all the signals of the resulting closed‐loop system are bounded with system output practically tracking the reference trajectory. Finally, simulation results are provided for two specified robot systems to validate the effectiveness of the theoretical results.
An end‐to‐end steel surface defect detection approach via Swin transformer
Authors: Tang Bo , Song Zi‐Kai , Sun Wei , Wang Xing‐Dong 
Published: December 2022

AbstractDifferent from most current studies using convolutional neural network (CNN), a deep learning detection method for steel plate surface defects based on Transformer is researched. This paper presents a process, network structure and detection method for steel strip surface defect detection. A Swin Transformer was used to extract hierarchical features in the detection system. Then feature pyramid networks (FPN) was used to fuse the above features to form multi‐scale feature maps, and region proposal network (RPN) was adopted to generate the generate region of interest (ROI) of defects. Finally, the ROI head was used to generate defect category information and its precise location. Here, ablation experiments were conducted to explore the impact of different backbone networks and the number of stages of Swin Transformer and FPN on target detection performance to prove the rationality and efficiency of the network. On the NEU‐DET dataset, the proposed algorithm achieved 81.1% mAP, which is higher than that of classic CNN detection methods such as Faster R‐CNN, SSD, Yolo v3 and RepPoints.
A worker‐and‐system trade‐off model for rebalancing free‐float bike sharing systems: A mixed rebalancing strategy
Authors: Wei Zhonghua , Wang Mingqian , Wang Shaofan 
Published: December 2022

AbstractBecause of imbalanced spatial‐temporal user demands, situations with no available bikes or docks often occur. In order to better response to user demands, many researchers are devoted to rebalancing bike sharing systems (BSS). Existing researches focus primarily on rebalancing BSS by trucks or by workers individually, resulting in two issues: (a) the high cost and massive emissions associated with trucks‐based rebalancing; and (b) the low efficiency associated with workers‐based rebalancing. This paper combines two rebalancing methods together and proposes a mixed rebalancing strategy to tackle the aforementioned issues. This strategy rebalances free‐floating BSS (FBSS) by trucks on the eve of peak hours and by workers during peak hours, in which the worker‐based rebalancing plan is given by a worker‐and‐system trade‐off model which simultaneously considers the impact of workers' costs and real‐time inventory of stations. A case study is conducted on two scenarios of the Beijing Mobike dataset: the Beigongdaximen subway station and the Jinyijiayuan residential area. The results show that our mixed rebalancing strategy can effectively rebalance FBSS by improving the following four indices: rebalancing cost, usage rate of shared bikes and docks, survival time of stations, and demand satisfaction.