bluuebunny/crossref_metadata_2025_split huggingface dataset:
Structural and biochemical characterization of Schlafen11 N-terminal domain
Authors: Hou Pengjiao , Hao Wei , Qin Bo , Li Mengyun , Zhao Rong , Cui Sheng 
Published: June 2023

Abstract
Schlafen11 (SLFN11) is one of the most studied Schlafen proteins that plays vital roles in cancer therapy and virus-host interactions. Herein, we determined the crystal structure of the Sus scrofa SLFN11 N-terminal domain (NTD) to 2.69 Å resolution. sSLFN11-NTD is a pincer-shaped molecule that shares an overall fold with other SLFN-NTDs but exhibits distinct biochemical characteristics. sSLFN11-NTD is a potent RNase cleaving type I and II tRNAs and rRNAs, and with preference to type II tRNAs. Consistent with the codon usage-based translation suppression activity of SLFN11, sSLFN11-NTD cleaves synonymous serine and leucine tRNAs with different efficiencies in vitro. Mutational analysis revealed key determinates of sSLFN11-NTD nucleolytic activity, including the Connection-loop, active site, and key residues essential for substrate recognition, among which E42 constrains sSLFN11-NTD RNase activity, and all nonconservative mutations of E42 stimulated RNase activities. sSLFN11 inhibited the translation of proteins with a low codon adaptation index in cells, which mainly dependent on the RNase activity of the NTD because E42A enhanced the inhibitory effect, but E209A abolished inhibition. Our findings provide structural characterization of an important SLFN11 protein and expand our understanding of the Schlafen family.
Pervasive effects of RNA degradation on Nanopore direct RNA sequencing
Authors: Prawer Yair D J , Gleeson Josie , De Paoli-Iseppi Ricardo , Clark Michael B 
Published: June 2023

Abstract
Oxford Nanopore direct RNA sequencing (DRS) is capable of sequencing complete RNA molecules and accurately measuring gene and isoform expression. However, as DRS is designed to profile intact RNA, expression quantification may be more heavily dependent upon RNA integrity than alternative RNA sequencing methodologies. It is currently unclear how RNA degradation impacts DRS or whether it can be corrected for. To assess the impact of RNA integrity on DRS, we performed a degradation time series using SH-SY5Y neuroblastoma cells. Our results demonstrate that degradation is a significant and pervasive factor that can bias DRS measurements, including a reduction in library complexity resulting in an overrepresentation of short genes and isoforms. Degradation also biases differential expression analyses; however, we find that explicit correction can almost fully recover meaningful biological signal. In addition, DRS provided less biased profiling of partially degraded samples than Nanopore PCR-cDNA sequencing. Overall, we find that samples with RNA integrity number (RIN) &gt; 9.5 can be treated as undegraded and samples with RIN &gt; 7 can be utilized for DRS with appropriate correction. These results establish the suitability of DRS for a wide range of samples, including partially degraded in vivo clinical and post-mortem samples, while limiting the confounding effect of degradation on expression quantification.
Spinal meningiomas
Authors: Hohenberger Christoph , Hau Peter , Schebesch Karl-Michael , Kölbl Oliver , Riemenschneider Markus J , Pohl Fabian , Proeschold Martin , Schmidt Nils Ole 
Published: June 2023

Abstract
Spinal meningiomas (SM) are lesions with a mostly favorable oncological and surgical prognosis and a low incidence of tumor recurrence. SM account for approximately 1.2–12.7% of all meningiomas and 25% of all spinal cord tumors. Typically, SM are located in the intradural extramedullary space. SM grow slowly and spread laterally into the subarachnoid space, stretching and sometimes incorporating the surrounding arachnoid but rarely the pia. Standard treatment is surgery with the primary aims of achieving complete tumor resection as well as improving and recovering neurologic function. Radiotherapy may be considered in case of tumor recurrence, for challenging surgical cases, and for patients with higher-grade lesions (World Health Organization grade 2 or 3); however, radiotherapy is mostly used as an adjuvant therapy for SM. New molecular and genetic profiling increases the understanding of SM and may uncover additional treatment options.
Metasurfaces: physics and applications in wireless communications
Authors: Ataloglou Vasileios G , Taravati Sajjad , Eleftheriades George V 
Published: June 2023

ABSTRACT
The ever increasing number of wireless devices and systems has led to a crowded spectrum and increased the demand for versatile and multi-functional wireless apparatuses. Recently, metasurfaces have been explored as a prominent technological solution to the current paradigm of spectrum scarcity by opportunistically sharing the spectrum with various users. In general, metasurfaces are passive/dynamic, ultra-compact, multi-functional and programmable structures that are capable of both reciprocal and nonreciprocal signal-wave transmissions. The controllability and programmability of such metasurfaces are governed through DC bias and occasionally a radio-frequency modulation applied to the active components of the unit cells of the metasurface, e.g. diodes and transistors. This article overviews some of the recently proposed passive and dynamic metasurfaces and shows that metasurfaces can enhance the performance of wireless communication systems thanks to their unique physical features such as real-time signal coding, nonreciprocal-beam radiation, nonreciprocal beamsteering amplification and advanced pattern-coding multiple access communication.
Cytisine Versus Varenicline for Smoking Cessation in a Primary Care Setting: A Randomized Non-inferiority Trial
Authors: Oreskovic Tin , Percac-Lima Sanja , Ashburner Jeffrey M , Tiljak Hrvoje , Rifel Janez , Klemenc Ketiš Zalika , Oreskovic Stjepan 
Published: June 2023

Abstract

Introduction
A smoking-cessation program was implemented as a randomized non-inferiority trial in primary care practices in Croatia and Slovenia to investigate whether a standard 4-week treatment with cytisine was at least as effective and feasible as a standard 12-week treatment with varenicline in helping smokers quit.

Aims and Methods
Out of 982 surveyed smokers, 377 were recruited to the non-inferiority trial: 186 were randomly assigned to cytisine and 191 to varenicline treatment. The primary cessation outcome was 7-day abstinence after 24 weeks, while the primary feasibility outcome was defined by adherence to the treatment plan. We also compared the rates of adverse events between the two treatment groups.

Results
The cessation rate after 24 weeks was 32.46% (62/191) in the varenicline group and 23.12% (43/186) in the cytisine group (odds ratio [OR]: 0.63, 95% credible interval [CI]: 0.39 to 0.98). Of 191 participants assigned to varenicline treatment 59.16% (113) were adherent, while 70.43% (131 of 186) were adherent in the cytisine group (OR: 1.65, 95% CI: 1.07 to 2.56). Participants assigned to cytisine experienced fewer total (incidence rate ratio [IRR]: 0.59, 95% CI: 0.43 to 0.81) and fewer severe or more extreme adverse events (IRR: 0.72, 95% CI: 0.35 to 1.47).

Conclusions
This randomized non-inferiority trial (n = 377) found the standard 4-week cytisine treatment to be less effective than the standard 12-week varenicline treatment for smoking cessation. However, adherence to the treatment plan, ie, feasibility, was higher, and the rate of adverse events was lower among participants assigned to cytisine treatment.

Implications
The present study found the standard 12 weeks of varenicline treatment to be more effective than the standard 4 weeks of cytisine treatment for smoking cessation in a primary care setting in Croatia and Slovenia. Participants assigned to cytisine, however, had a higher adherence to the treatment plan and a lower rate of adverse events. Estimates from the present study may be especially suitable for generalizations to high-smoking prevalence populations in Europe. Given the much lower cost of cytisine treatment, its lower rate of adverse events, and higher feasibility (but its likely lower effectiveness with the standard dosage regimen), future analyses should assess the cost-effectiveness of the two treatments for health policy considerations.
Home Care Aides’ Perspectives of Occupational Tobacco Smoke Exposure: A Q Methodology Study
Authors: Huang Su-Fei , Liao Jung-Yu 
Published: June 2023

Abstract

Introduction
The increased growth of older adults has generated demand for home care aides (HCAs). Occupational tobacco smoke exposure (OTSE) may risk their health which should be paid attention to.

Aims and Methods
This study explored the HCAs’ perspectives of OTSE to inform health promotion programs catering to individual needs. A two-stage Q methodology was employed for data collection and analysis. Thirty-nine Q statements were extracted in the first stage and then 51 HCAs with OTSE were recruited to complete Q sorting in the second stage. PQ Method software was used for data analysis. Principal component analysis was performed to determine the most appropriate number of factors.

Results
The five factors identified from HCAs’ perspective of OTSE explained 51% of the variance. The HCAs agreed that OTSE could increase cancer risk. The HCAs with factor I did not care about OTSE, tending to complete their work. The HCAs with factor II agreed with the health hazards of OTSE, but did not know how to help their clients stop smoking. The HCAs with factor III cared about OTSE but were afraid of disrupting the client-provider relationship. The HCAs with factor IV regarded OTSE as a priority for occupational interventions whereas the HCAs with factor V did not think OTSE was an issue and believed that they could balance work and OTSE health hazards.

Conclusions
Our findings will inform the design of home care pre-service and on-the-job training courses. Long-term care policies should be developed to promote smoke-free workplaces.

Implications
There are five types of HCAs’ perspectives on OTSE. The tailor interventions can be developed to help them avoid the OTSE (eg, opening windows for ventilation or using air purification equipment) and have an OTSE-free space.
Inundative, Dry-Powder, Inhaled Measles Vaccination to Prevent Deaths of Young Children in War-torn Regions
Authors: Sievers Benjamin L , Sievers Robert E , Sievers Eric L 
Published: June 2023

Abstract
Children living in war-torn and geographically remote regions often die from measles due to undervaccination. Protective community immunity could be safely improved through the comprehensive use of small, inexpensive, easy-to-use, dry-powder aerosolized measles vaccination inhalers. Influential local community members could be engaged to provide risk counseling and inform their peers of measles risks to inspire vaccine uptake. Vaccination by inhaled live attenuated measles vaccine has been shown to be safe and protective among several million research subjects and omits (1) needles, syringes, glass vials, and specialized disposal systems; (2) deadly vaccine reconstitution errors; (3) cold chain technology to protect temperature-sensitive vaccine; (4) vaccine wastage associated with suboptimal use of multidose vials; (5) trained vaccinators; (6) food, housing, and transportation costs associated with centralized vaccination campaigns; and (7) risk of violence to vaccinators and associated staff. All elements for inhaler-based measles vaccination are readily available. Dry-powder measles vaccine inhalers can be assembled and distributed to save lives.
Latent Tuberculosis Screening Cascade for Non–US-Born Persons in a Large Health System
Authors: Shapiro Adrienne E , Gupta Ayushi , Lan Kristine , Kim H Nina 
Published: June 2023

Abstract
Review of electronic health records revealed substantial drop-off at each stage of the latent tuberculosis infection (LTBI) care cascade among non–US-born persons in an academic primary care system. Of 5148 persons eligible for LTBI screening, 1012 (20%) had an LTBI test, and 140 (48%) of 296 LTBI-positive persons received LTBI treatment.
An Analysis of Clinical and Pathologic Features, RecurIndex Genomic Profiles, and Survival Outcomes in HER2-Low Breast Cancer
Authors: Hui Tianli , Li Sainan , Wang Huimin , Ma Xuejiao , Du Furong , Gao Wei , Yang Shan , Sang Meixiang , Li Ziyi , Ding Ran , Liu Yueping , Geng Cuizhi 
Published: June 2023

Abstract

Background
In recent years, breast cancer has become the most common cancer in the world, increasing women’s health risks. Approximately 60% of breast cancers are categorized as human epidermal growth factor receptor 2 (HER2)-low tumors. Recently, antibody-drug conjugates have been found to have positive anticancer efficacy in patients with HER2-low breast cancer, but more studies are required to comprehend their clinical and molecular characteristics.

Methods
In this study, we retrospectively analyzed the data of 165 early breast cancer patients with pT1-2N1M0 who had undergone the RecurIndex testing. To better understand HER2-low tumors, we investigated the RecurIndex genomic profiles, clinicopathologic features, and survival outcomes of breast cancers according to HER2 status.

Results
First, there were significantly more hormone receptor (HR)-positive tumors, luminal-type tumors, and low Ki67 levels in the HER2-low than in the HER2-zero. Second, RI-LR (P = .0294) and RI-DR (P = .001) scores for HER2-low and HER2-zero were statistically significant. Third, within HER2-negative disease, HR-positive/HER2-low tumors showed highest ESR1, NFATC2IP, PTI1, ERBB2, and OBSL1 expressions. Fourth, results of the survival analysis showed that lower expression of HER2 was associated with improved relapse-free survival for HR-positive tumors, but not for HR-negative tumors.

Conclusions
The present study highlights the unique features of HER2-low tumors in terms of their clinical characteristics as well as their gene expression profiles. HR status may influence the prognosis of patients with HER2-low expression, and patients with HR-positive/HER2-low expression may have a favorable outcome.
A Single-Arm, Open-Label Phase II Study of ONC201 in Recurrent/Refractory Metastatic Breast Cancer and Advanced Endometrial Carcinoma
Authors: Atkins Sarah L P , Greer Yoshimi Endo , Jenkins Sarah , Gatti-Mays Margaret E , Houston Nicole , Lee Sunmin , Lee Min-Jung , Rastogi Shraddha , Sato Nahoko , Burks Christina , Annunziata Christina M , Lee Jung-Min , Nagashima Kunio , Trepel Jane B , Lipkowitz Stanley , Zimmer Alexandra S 
Published: June 2023

Abstract

Background
ONC201 is a small molecule that can cause nonapoptotic cell death through loss of mitochondrial function. Results from the phase I/II trials of ONC201 in patients with refractory solid tumors demonstrated tumor responses and prolonged stable disease in some patients.

Methods
This single-arm, open-label, phase II clinical trial evaluated the efficacy of ONC201 at the recommended phase II dose (RP2D) in patients with recurrent or refractory metastatic breast or endometrial cancer. Fresh tissue biopsies and blood were collected at baseline and at cycle 2 day 2 for correlative studies.

Results
Twenty-two patients were enrolled; 10 patients with endometrial cancer, 7 patients with hormone receptor–positive breast cancer, and 5 patients with triple-negative breast cancer. The overall response rate was 0%, and the clinical benefit rate, defined by complete response (CR) + partial response (PR) + stable disease (SD), was 27% (n = 3/11). All patients experienced an adverse event (AE), which was primarily low grade. Grade 3 AEs occurred in 4 patients; no grade 4 AEs occurred. Tumor biopsies did not show that ONC201 consistently induced mitochondrial damage or alterations in tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) or the TRAIL death receptors. ONC201 treatment caused alterations in peripheral immune cell subsets.

Conclusion
ONC201 monotherapy did not induce objective responses in recurrent or refractory metastatic breast or endometrial cancer at the RP2D dose of 625 mg weekly but had an acceptable safety profile (ClinicalTrials.gov Identifier: NCT03394027).
Pearls of wisdom: Updated skill-specific parenting strategies in the first 6 years
Authors: Dosman Cara , Koscielnuk Dorrie , Gallagher Sheila 
Published: June 2023

Abstract
This article provides knowledge translation on up-to-date parenting strategies (pearls of wisdom). These pearls support the development of specific skills in children from birth through 5 years of age. Paediatricians have indicated that they feel inadequately trained in providing parenting guidance. This article could be used by family physicians, community health nurses, nurse practitioners, and paediatricians as an office reference when providing anticipatory parenting guidance and when there are parent or clinician concerns that relate to various developmental stages. Included are general tips for guiding parents and pearls that support self-regulation, attachment, perspective taking, and developing the child’s sense of self. The present review links pearls to specific reference sources in the literature which cite developmental standards and advice. Evidence-based parenting programs are cited for children with more complex presentations due to developmental, regulatory, or behavioural disorders, temperament individual differences, or limited parenting support. Website links for parents are recommended, and Parenting Pearls handouts provided, to solidify understanding and follow through.
Assemblages of pelagic thaliaceans in oceanographic features at the tropical-temperate transition zone of a western boundary current
Authors: Pitt Kylie A , Lawley Jonathan W , Hinchliffe Charles , Matis Paloma A , OlguÍn-Jacobson Carolina , Arafeh-Dalmau Nur , Lindholm Pauline , Arnold Jade , Suthers Iain M 
Published: June 2023

AbstractMesoscale oceanographic features influence the composition of zooplankton. Cyclonic eddies can promote upwelling and production of gelatinous zooplankton, which play critical roles in ocean biogeochemical cycling. We examined variation in assemblages of thaliaceans (salps, doliolids and pyrosomes) among mesoscale oceanographic features at the tropical-temperate boundary of the East Australian Current (EAC) in Spring 2019 and Autumn 2021. The influence of cyclonic eddies was examined in a large offshore cyclonic eddy in 2019 and a newly formed frontal eddy in 2021. Pyrosomes were most abundant in the offshore EAC jet, and salps and doliolids were most abundant in coastal features, including within eddies that were transported offshore. In 2019, Salpa fusiformis increased 4-fold over 8 days in the large cyclonic eddy, and in 2021, doliolids increased &gt; 50-fold over 2 weeks in a chlorophyll-rich coastal eddy while abundances of other thaliaceans remained unchanged or decreased. Correlations between abundances of thaliaceans and chlorophyll-a concentrations across the 102 samples collected during both voyages revealed that doliolids occupy a wider range of chlorophyll-a concentrations than salps. Our observations indicate that doliolids thrive in productive shelf environments, salps occur in less productive shelf waters and pyrosomes are most abundant in oligotrophic waters of the south Coral Sea.
A systematic review of the relationship between household food insecurity and childhood undernutrition
Authors: Lye Chuan Way , Sivasampu Sheamini , Mahmudiono Trias , Majid Hazreen Abdul 
Published: June 2023

AbstractBackgroundThis systematic review aims to investigate the association between household food insecurity (HFIS) and undernutrition in children under 5 years old, thereby informing policymakers on the vital factors needed to be considered in tailoring an effective strategy to tackle childhood undernutrition and ultimately HFIS.MethodsWe performed a systematic review that examined household food insecurity among the undernourished under-five children. PubMed, Cochrane, EBSCO Host, Web of Science and Cumulative Index to Nursing and Allied Health Literature were searched from 1 January 2012 to 1 April 2022, for relevant articles. Outcome measures were stunting, underweight or wasting. Of the 2779 abstracts screened, 36 studies that fulfilled the inclusion and exclusion criteria were included. A range of tools had been used to measure HFIS, the most common being Household Food Insecurity Access Scale. HFIS has been found to be significantly associated with undernutrition, particularly stunting and underweight. This is observed proportionately across all national income levels.ConclusionsSustainable and inclusive economic growth, which aims to reduce income, education and gender inequality, should be a key policy goal in the minimizing food insecurity and childhood undernutrition. Multisectoral interventions are needed to address these issues.
The course of cytokine and chemokine gene expression in clinically suspect arthralgia patients during progression to inflammatory arthritis
Authors: Heutz Judith W , Rogier Cleo , Niemantsverdriet Ellis , van den Eeden Susan J F , de Jong Pascal H P , Lubberts Erik , Geluk Annemieke , van der Helm-van Mil Annette H M 
Published: June 2023

Abstract

Objectives
Autoantibody responses increase years before the onset of inflammatory arthritis (IA) and are stable during transitioning from clinically suspect arthralgia (CSA) to IA. Cytokine and chemokine levels also increase years before IA onset. However, the course in the at-risk stage of CSA during progression to disease or non-progression is unknown. To increase the understanding of processes mediating disease development, we studied the course of cytokine, chemokine and related receptors gene expression in CSA patients during progression to IA and in CSA patients who ultimately did not develop IA.

Methods
Whole-blood RNA expression of 37 inflammatory cytokines, chemokines and related receptors was determined by dual-colour reverse transcription multiplex ligation-dependent probe amplification in paired samples of CSA patients at CSA onset and either at IA development or after 24 months without IA development. ACPA-positive and ACPA-negative CSA patients developing IA were compared at CSA onset and during progression to IA. Generalised estimating equations tested changes over time. A false discovery rate approach was applied.

Results
None of the cytokine/chemokine genes significantly changed in expression between CSA onset and IA development. In CSA patients without IA development, G-CSF expression decreased (P = 0.001), whereas CCR6 and TNIP1 expression increased (P &lt; 0.001 and P = 0.002, respectively) over a 2 year period. Expression levels in ACPA-positive and ACPA-negative CSA patients who developed IA were similar.

Conclusion
Whole-blood gene expression of assessed cytokines, chemokines and related receptors did not change significantly from CSA to IA development. This suggests that changes in expression of these molecules may not be related to the final process of developing chronicity and may have occurred preceding CSA onset. Changes in gene expression in CSA patients without IA development may provide clues for processes related to resolution.