bluuebunny/crossref_metadata_2025_split huggingface dataset:
Slim the face or not: 3D change of facial soft and hard tissues after third molars extraction: a pilot study
Authors: Wang Xin-Wen , Yang Hui-Fang , Wang En-Bo , Cui Xin-Yu , Zhao Yi-Jiao , Jiang Jiu-Hui 
Published: July 2023

Abstract
Background
Whether slim the face or not after removed third molars is the concern of some orthodontic treatment candidates. The aim of this article is to explore the volume changes of facial soft and hard tissues after third molars extraction, as well as develop a reproducible clinical protocol to precisely assess facial soft tissue volume change.

Methods
A non-randomized, non-blind, self-controlled pilot study was conducted. 24 adults aged 18–30 had ipsilateral third molars extracted. The body weight change was controlled within 2 kg. Structured light scans were taken under a standardized procedure pre-extraction (T0), three (T1), and six (T2) months post-extraction; CBCTs were taken at T0 and T2. The projection method was proposed to measure the soft tissue volume (STV) and the soft tissue volume change (STVC) by the Geomagic software. The hard tissue volume change (HTVC) was measured in the Dragonfly software.

Results
The final sample size is 23, including 5 males (age 26.6 ± 2.5 years) and 18 females (age 27.3 ± 2.5 years). The HTVC was − 2.33 ± 0.46ml on the extraction side. On the extraction side, the STV decreased by 1.396 (95% CI: 0.323–2.470) ml (P < 0.05) at T1, and increased by 1.753 (95% CI: -0.01-3.507) ml (P = 0.05) at T2. T2 and T0 had no difference (P > 0.05). The inter and intra-raters ICC of the projection method was 0.959 and 0.974. There was no correlation between the STVC and HTVC (P > 0.05).

Conclusions
After ipsilateral wisdom teeth extraction, the volume of hard tissue on the extraction side reduces, and the volume of facial soft tissue does not change evidently. However, further research with large sample size is still needed. The STV measurement has excellent repeatability. It can be extended to other interested areas, including forehead, nose, paranasal, upper lip, lower lip and chin, which is meaningful in the field of orthodontics and orthopedics.

Trial registration
ChiCTR, ChiCTR1800018305 (11/09/2018), http://www.chictr.org.cn/showproj.aspx?proj=28868.
A prognostic model using FIGO 2018 staging and MRI-derived tumor volume to predict long-term outcomes in patients with uterine cervical squamous cell carcinoma who received definitive radiotherapy
Authors: Zang Lele , Chen Qin , Lin An , Chen Jian , Zhang Xiaozhen , Fang Yi , Wang Min 
Published: July 2023

Abstract
Background
Uterine cervical carcinoma is a severe health threat worldwide, especially in China. The International Federation of Gynecology and Obstetrics (FIGO) has revised the staging system, emphasizing the strength of magnetic resonance imaging (MRI). We aimed to investigate long-term prognostic factors for FIGO 2018 stage II–IIIC2r uterine cervical squamous cell carcinoma following definitive radiotherapy and establish a prognostic model using MRI-derived tumor volume.

Methods
Patients were restaged according to the FIGO 2018 staging system and randomly grouped into training and validation cohorts (7:3 ratio). Optimal cutoff values of squamous cell carcinoma antigen (SCC-Ag) and tumor volume derived from MRI were generated for the training cohort. A nomogram was constructed based on overall survival (OS) predictors, which were selected using univariate and multivariate analyses. The performance of the nomogram was validated and compared with the FIGO 2018 staging system. Risk stratification cutoff points were generated, and survival curves of low-risk and high-risk groups were compared.

Results
We enrolled 396 patients (training set, 277; validation set, 119). The SCC-Ag and MRI-derived tumor volume cutoff values were 11.5 ng/mL and 28.85 cm3, respectively. A nomogram was established based on significant prognostic factors, including SCC-Ag, poor differentiation, tumor volume, chemotherapy, and FIGO 2018 stage. Decision curve analysis indicated that the net benefits of our model were higher. The high-risk group had significantly shorter OS than the low-risk group in both the training (p < 0.0001) and validation sets (p = 0.00055).

Conclusions
Our nomogram predicted long-term outcomes of patients with FIGO 2018 stage II–IIIC2r uterine cervical squamous cell carcinoma. This tool can assist gynecologic oncologists and patients in treatment planning and prognosis.
H3K27me3 of Rnf19a promotes neuroinflammatory response during Japanese encephalitis virus infection
Authors: Zhu Shuo , Tao Mengying , Li Yunchuan , Wang Xugang , Zhao Zikai , Liu Yixin , Li Qi , Li Qiuyan , Lu Yanbo , Si Youhui , Cao Shengbo , Ye Jing 
Published: July 2023

AbstractHistone methylation is an important epigenetic modification that affects various biological processes, including the inflammatory response. In this study, we found that infection with Japanese encephalitis virus (JEV) leads to an increase in H3K27me3 in BV2 microglial cell line, primary mouse microglia and mouse brain. Inhibition of H3K27me3 modification through EZH2 knockdown and treatment with EZH2 inhibitor significantly reduces the production of pro-inflammatory cytokines during JEV infection, which suggests that H3K27me3 modification plays a crucial role in the neuroinflammatory response caused by JEV infection. The chromatin immunoprecipitation-sequencing (ChIP-sequencing) assay revealed an increase in H3K27me3 modification of E3 ubiquitin ligases Rnf19a following JEV infection, which leads to downregulation of Rnf19a expression. Furthermore, the results showed that Rnf19a negatively regulates the neuroinflammatory response induced by JEV. This is achieved through the degradation of RIG-I by mediating its ubiquitination. In conclusion, our findings reveal a novel mechanism by which JEV triggers extensive neuroinflammation from an epigenetic perspective.
Circ-STC2 promotes the ferroptosis of nucleus pulposus cells via targeting miR-486-3p/TFR2 axis
Authors: Xiong Liangping , Li Xiaoyan , Hua Xi , Qian Zhonglai 
Published: July 2023

Abstract
Background
Low back pain (LBP) has become the second leading cause of disability worldwide, which has brought great economic burden to people. It is generally believed that intervertebral disc degeneration (IDD) is the main cause of LBP. This study aimed to explore the role of circ-STC2 in the pathogenesis of IDD.

Methods
Nucleus pulposus cells (NPCs) were treated with T-Butyl Hydrogen Peroxide (TBHP) to establish IDD model in vitro. RT-qPCR was performed to detect mRNA expressions. The cell viability was detected with CCK-8 assay. The levels of lactate dehydrogenase (LDH), malondialdehyde (MDA), Fe2+ and glutathione (GSH) of NPCs were measured by corresponding kits. The protein expressions were determined by western blot. Dual-luciferase reporter and RNA pull-down assays were conducted to verify the relationship between circ-STC2 or transferrin recepto 2 (TFR2) and miR-486-3p.

Results
Circ-STC2 and TFR2 expressions were up-regulated in IDD tissues, and miR-486-3p expression was down-regulated. Knockdown of circ-STC2 promoted the cell viability and inhibited the ferroptosis of the NPCs. The GSH levels, and glutathione peroxidase 4 (GPX4) and solute carrier family 7 member 11 (SLC7A11) protein expressions were increased, the LDH, MDA and Fe2+ levels and achaete-scute complexlike 4 (ASCL4) protein expressions were decreased after circ-STC2 knockdown. Knockdown of miR-486-3p abrogated the si-circ-STC2 effects and overexpression of TFR2 reversed the miR-486-3p mimic effects.

Conclusions
Circ-STC2 inhibits the cell viability, induced the ferroptosis of the TBHP treated NPCs via targeting miR-486-3p/TFR2 axis.
DCF versus doublet chemotherapy as first-line treatment of advanced squamous anal cell carcinoma: a multicenter propensity score-matching study
Authors: Kim Stefano , Vendrely Véronique , Saint Angélique , André Thierry , Vaflard Pauline , Samalin Emmanuelle , Pernot Simon , Bouché Oliver , Zubir Mustapha , Desrame Jérôme , de la Fouchardière Christelle , Smith Denis , Ghiringhelli François , Vienot Angélique , Jacquin Marion , Klajer Elodie , Nguyen Thierry , François Éric , Taieb Julien , Le Malicot Karine , Vernerey Dewi , Meurisse Aurélia , Borg Christophe 
Published: July 2023

AbstractTriplet DCF (docetaxel, cisplatin and 5-flurouracil) and doublet CP/CF (carboplatin and paclitaxel/cisplatin and 5-fluorouracil) regimens were prospectively evaluated in advanced squamous anal cell carcinoma (SCCA), and validated as standard treatments. Even though the high efficacy and good tolerance of DCF regimen were confirmed in 3 independent prospective trials, doublet CP regimen is still recommended in several guidelines based in its better safety profile with similar efficacy compared to CF regimen. We performed a propensity score-adjusted method with inverse probability of treatment weighted (IPTW) and matched case control (MCC) comparison among patients with metastatic or non-resectable locally advanced recurrent SCCA, treated with chemotherapy as first line regimen. The primary endpoint was the overall survival (OS), and the secondary endpoint was the progression-free survival (PFS). 247 patients were included for analysis. 154 patients received DCF and 93 patients received a doublet regimen. The median OS was 32.3 months with DCF and 18.3 months with doublet regimens (HR 0.53, 95%CI 0.38–0.74; p = 0.0001), and the median PFS was 11.2 months with DCF versus 7.6 months with doublet regimens (HR 0.53, 95%CI 0.39–0.73; p < 0.0001). The hazard ratios by IPTW and MCC analyses were 0.411 (95% CI, 0.324–0.521; p < 0.0001) and 0.406 (95% CI, 0.261–0.632; p < 0.0001) for OS, and 0.466 (95% CI, 0.376–0.576; p < 0.0001) and 0.438 (95% CI, 0.298–0.644; P < 0.0001) for PFS. The triplet DCF regimen provides a high and significant benefit in OS and PFS over doublet regimens, and should be considered as upfront treatment for eligible patients with advanced SCCA.
ANALISA KEKUATAN PONDASI Z – PELLER KARENA ADANYA PERUBAHAN KONSTRUKSI PADA KAPAL TUG BOAT ANOMAN V DENGAN METODE ELEMEN HINGGA
Authors: Jatmiko Sukanto , Handoyo Tunjung Widyatmo 
Published: July 2023

Kapal Tug Boat Anoman V adalah kapal tandu milik PT. Pelabuhan Indonesia III yang beroperasi di Pelabuhan Tanjung Perak Surabaya. Kapal tersebut mempunyai mesin induk sebesar 2 x 1000 HP untuk menunjang operasionalnya yang di dukung oleh sistem propulsi Z – peller sebagai penggeraknya. Untuk menopang Z – peller tersebut di gunakan pondasi yang disusun oleh beberapa pelat lingkar dari material KI-A 36. Pada Tugas Akhir ini akan dilakukan analisa local stress yang terjadi pada pondasi Z - peller kapal Tug boat Anoman V dengan bantuan program numerik finite element method (FEM). Analisa yang digunakan adalah analisa beban statis yang berasal dari berat permesinan Z – peller dan beban dari hambatan propulsi kapal. Analisa tersebut bertujuan untuk mengetahui karakteristik dan letak tegangan terbesar dari struktur konstruksi pondasi Z - peller berdasarkan dua variasi kondisi keadaan kapal yaitu pada saat sandar dan pada kecepatan maksimum. Analisa tersebut menggunakan metode elemen hingga dengan menggunakan matrik tetrahedral untuk membagi – bagi menjadi elemen yang kecil. Hasil analisa menggunakan program numerik FEM didapatkan hasil makimum stress pada sistem konstruksi pondasi Z - peller sebesar (σ) = 22,7 N/mm2 dan deformasi sebesar 0,00763 mm. Perhitungan faktor keamanan didapat nilai sebesar 17,2 pada lokasi node 3459 pada saat kondisi kecepatan maksimum. Kondisi ini aman karena nilai faktor keamanan lebih dari 1 berdasarkan ketentuan dari BKI. Hasil dari perhitungan analisa tegangan maksimum tersebut dilakukan validasi model, software, dan analitik untuk mengetahui tingkat ralat nisbi dari analisa kekuatan pondasi Z – peller.
ANALISA PENGARUH ALIRAN FLUIDA YANG DITIMBULKAN OLEH GERAKAN PUTARAN PROPELLER PADA KAPAL IKAN TERHADAP TEKANAN PROPELLER DENGAN PENDEKATAN CFD
Authors: Samuel Samuel , Hafiz Dian 
Published: July 2023

Dalam operasinya dilaut, suatu kapal harus memiliki kemampuan untuk mempertahankan kecepatan dinas (Vs) seperti yang direncanakan. Hal ini mempunyai arti bahwa, kapal haruslah mempunyai rancangan sistem propulsi yang dapat mengatasi keseluruhan gaya-gaya hambat yang terjadi agar memenuhi standar kecepatan dinasnya. Penelitian untuk meningkatkan daya dorong propeller dari tahun ketahun terus dilakukan dengan meningkatakan efisiensi propeller. Tapi dari sisi lain usaha untuk meneliti dimana konsentrasi tegangan yang diakibatkan daya dorong propeller masih kurang dilakukan Untuk tugas akhir ini analisa yang dilakukan adalah untuk mengetahui distribusi tekanan pada propeller dan juga maximum stress yang terjadi pada propeller. Proses pembuatan model geometri dibantu dengan menggunakan software CAD dan analisanya dilakukan dengan mengunakan pendekatan CFD (Computational Fluid Dynamic) sebagai sarana visualisasi.Analisa dilakukan dengan memvariasikan putaran propeller pada 100, 200, 300, 400, 500 rpm. Berdasarkan hasil perhitungan dan hasil running software CFD yang telah dilakukan didapatkan hasil perbedaan tekanan dimana pada tiap putarannya semakin meningkat. Tekanan paling besar terjadi pada putaran 500 rpm yaiutu sebesar 28169,72 N. Dengan menggunakan software MSC NASTRAN didapatkan nilai maksimum strees sebesar 13,1 N/mm2 akan tetapi material ini masih dalam batas aman karena hasil perhitungan safety factor material bahan didapat nilai 33,87.
Dissimilar effects of stereoisomers and racemic hydroxychloroquine on Ca2+ oscillations in human induced pluripotent stem cell‐derived cardiomyocytes
Authors: Janicki Piotr K. , Singh Amandeep , Sharma Arun K. , Ruiz‐Velasco Victor 
Published: July 2023

AbstractAll currently employed pharmaceutical formulations of hydroxychloroquine (HCQ) sulfate are a racemate, consisting of equal parts mixture of two stereoisomers: R(−)HCQ and S(+)HCQ sulfates. The aims of the current study were first, to obtain and characterize pure HCQ enantiomers. The separation and purification of free base HCQ enantiomers from the racemate form were performed using semi‐preparative chiral high‐performance liquid chromatography. Second, we compared the pharmacological properties of both optical isomers and racemic mixture on the intracellular Ca2+ oscillations employing an in vitro model of human cardiomyocytes derived from induced pluripotent stem cells (iPSCs). The results of the pharmacological investigations indicate that the racemic and pure stereoisomer forms of HCQ sulfate exhibited a dose‐dependent inhibition of spontaneous Ca2+ oscillations (as measured from their frequency and Ca2+ peak widths) in cardiomyocytes 5–45 min following exposure. In addition, the concentration‐response relationships for all three compounds indicated that the rank order of potency (IC50) was R(−)HCQ >racemic HCQ >S(+)HCQ for the frequency of the Ca2+ oscillations and width of Ca2+ peaks for all time points examined. These studies indicate that both R(−) and S(+) stereoisomers exhibit differing pharmacological actions on hiPSC cardiomyocytes, with the former effecting a greater potency on cell Ca2+ handling.
Introduction to bioimaging‐based spatial multi‐omic novel methods
Authors: Yan Yan , Yang Liheng , Meng Leyuan , Su Haochen , Zhou Cheng , Yu Le , Li Zhengtu , Zhang Xu , Cai Huihua , Gao Juntao 
Published: July 2023

In this review, we introduced five different multiplex FISH methods used for image‐based spatial multi‐omics: seqFISH+, merFISH, DNA seqFISH+, DNA merFISH, and MINA. We provided a systematic collective perspective to review these FISH methods that could significantly benefit researchers on conducting their studies in the field. Our study provided an informative survey on these multiplex FISH methods. Therefore, this review would provide better understanding for researchers in the community to help them select the proper method, in order to understand the molecular mechanism in life sciences.BackgroundSpatial multi‐omics are demonstrated to be a powerful method to assist researchers on genetic studies. In this review, bioimaging‐based spatial multi‐omics techniques such as seqFISH+, merFISH, integrated DNA seqFISH+, DNA merFISH, and MINA are introduced along with each technique’s probe design, development, and imaging processes.ResultsseqFISH employed 4–5 fluorophores to barcode and conducted multiple rounds of hybridization, in order that mRNA can be identified through color‐coding. seqFISH+ added 60 pseudo‐color and distributed them equally into three channels to enhance imaging power, in order that i.e., 24,000 genes can be imaged in total. merFISH utilized 4 out 16 Hamming distance to innovatively provide a robust error‐detecting method. MINA, a methodology combining merFISH (multiplexed error‐robust fluorescence in situ hybridization) and chromosomal tracing, enabled multiplexed genomic architecture imaged in mammalian single cells. Optical reconstruction of chromatin architecture (ORCA) a method that could conduct DNA path tracing in nanoscale manner with kilobase resolution, an FISH variation that improved genetic resolution, enable high‐precision fiducial registration and sequential imaging, and utilized Oligopaint probe to hybridize the short genomic region ranging from 2 to 10 kilobase. ORCA then prescribes these short section primary probes with individual barcodes to attach fluorophore and to be imaged.ConclusionThis review concentrated on providing a comprehensive overview for these spatial‐multi‐omics techniques with the intention on helping researchers on selecting appropriate technique for their research.
Chlamydia trachomatis and the HLA involvement in the development of infection and disease: a narrative review
Authors: Pintea-Trifu Martina-Luciana , Bâlici Ștefana , Siserman Costel-Vasile , Vică Mihaela-Laura , Matei Horea-Vladi 
Published: July 2023

Introduction. CT (Chlamydia trachomatis) is among the most common pathogens leading to sexually transmitted diseases. Considering the uncertain mechanism by which HLA polymorphisms influence the CT infection, reinfection, comorbidities or evolution and because there is no consensus regarding the alleles involved in the pathogenesis of the infection, we considered necessary to perform a review to summarize the current knowledge of HLA related to CT.
Methods. Pubmed was researched using key terms. Out of the 198 results found, we analyzed articles of all types which describe how the MHC, through HLA alleles, participates in the different stages of CT penetration in the body, including studies about cells or other molecules involved in the process.
Results. Almost 40% of the variation in the clinical course of CT infection depends on host genetic factors. There are haplotypes that influence the infection susceptibility/resistance, haplotypes that are involved in the recurrence of the infection, haplotypes that are related to tubal infertility, pelvic inflammatory disease development or trachoma. Antibody to Chsp60 (influenced by MHC genes) has been observed to correlate with late tissue-damaging sequelae. Toll-like receptors were found to increase the susceptibility to CT. The association of HLA-B27 creates susceptibility of reactive arthritis in the organisms infected by CT, but does not influence the carriage of CT.
Conclusion. We identified HLA haplotypes belonging both to MHC class l and ll, which influence different stages of CT infection. Genetic risk factors still need research, especially on Caucasians. Studies are moving towards designing a safe and effective vaccine.
Tensiones éticas, causas y consecuencias del síndrome de burnout en el personal de salud en tiempos de la Covid-19
Authors: García Uribe John Camilo , Espinal Espinal Luz Adriana , Canestero Montoya Irma del Carmen , Henao Villegas Santiago , Garzón Duque Maria Osley 
Published: July 2023

Esta es una revisión bibliométrica sobre el síndrome de burnout que afectó a profesionales de la salud en tiempos de la Covid-19, los conflictos éticos que lo preceden y suceden, y las estrategias para combatirlo. Metodología/ enfoque: se hizo una revisión bibliométrica en la que se identificaron, de manera sistemática, artículos de revisión, estudios transversales, metaanálisis y estudios cualitativos de todo el mundo. Una vez aplicados los filtros de selección, se analizaron los resultados con el método deliberativo de Diego Gracia. Resultados: se identificaron cuatro subtemas principales, incidencia del burnout durante la pandemia de la Covid-19, estresores éticos que favorecen al síndrome de burnout, consecuencias de este síndrome y estrategias para prevenirlo y combatirlo. Cada subtema se describe desde los ámbitos individuales, institucionales y sociales. Discusión/conclusiones: se argumenta con la teoría de Diego Gracia y se presenta una jerarquización de principios éticos implicados en el burnout; existen factores que favorecen la aparición de esta enfermedad profesional con un profundo calado bioético, en el que subyacen problemas de reconocimiento moral y económico, equidad, seguridad laboral, deber de cuidar, buena práctica médica y el bienestar del paciente, la familia y la sociedad en general. De igual, forma, las consecuencias del síndrome tienen repercusiones en los ámbitos sociales, institucionales e individuales, por lo que las intervenciones para su prevención y atención deben de igual forma tener un enfoque pluralista y diverso desde lo macro, meso y micro. En este sentido, es necesario fortalecer las estrategias de investigación y educación, para comprender, explicar y abordar el fenómeno de una forma integral.
The Influence of Calcium and Iron Supplementation in Pregnant Women to Affect Newborn Body Length in Bengkulu
Authors: Riyadi Agung , Ningsih Lisma , Jumiyati Jumiyati , Rahmadi Antun 
Published: July 2023

In 2021, the rate of stunting in Indonesia was 24.4%, and 21.1% happened in Bengkulu province. In 2020, the proportion of pregnant women who receive iron tablets in Bengkulu Province was 97%. Some regencies have a lower proportion, such as North Bengkulu at 87%. Pregnant women who suffer from the lack of iron and folic acid intake may experience anemia and impaired fetal growth. The aim of this study is to investigate the effect of calcium and iron supplementation in pregnant women on newborn body length in Bengkulu Province. This study used an experimental design with a post-test-only control group. The population was third semester pregnant women in Bengkulu Province. Subjects were taken using purposive sampling technique. The number of subjects involved was 29 in intervention group and control group. Instruments used ini this study were food frequency questionaire (FFQ), mid upper arm circumference tape, and a respondent characteristics questionnaire. Data analysis methods include independent t-test, correlation, and double linear regression. The consumption of iron tablets and calcium tablets (p=0.0001), as well as the nutritional status of pregnant women (p=0.0001), have significant effect on newborns body length. Calcium and iron tablets supplementation improve the nutritional status of pregnant women and increase newborn body length. However, it should be measured by using same controlling variables such as education, age, parity, protein intake, vitamin C intake, knowledge and gestational age of childbirth.