bluuebunny/crossref_metadata_2025_split huggingface dataset:
Mentoring as an effective form of formation and development of a young teacher's personality
Authors: Malinin V. A. , Povshednaya F. V. , Lebedeva O. V. , Pugachev A. V. 
Published: April 2023

Introduction. The proposed article is one of the opportunities to show the need to revive the institution of mentoring (tutoring) in the modern socio-economic conditions of the development of the national education system. Based on the research of scientists, the authors give a description of the main categories of research "mentoring" and "tutoring"; rethink different approaches to understanding their essence. Mentoring is defined by the authors as an effective form of formation and development of the personality of a young teacher. Materials and Methods. As research methods, the method of analysis and systematization of psychological-pedagogical theoretical-experimental and scientific-methodological studies of domestic scientists on the problem of mentoring, tutoring, support was used. Results. The historical and pedagogical analysis of the formation and development of the institute of mentoring in pre-revolutionary Russia, during the Soviet period of the development of the domestic education system and shows the possibilities of the revival of mentoring in modern Russia; the goals and objectives of the "School of Mentoring" in the conditions of the region are defined. The study of the problem of mentoring and tutoring forces a new assessment of their role and importance in the formation and development of the personality of a modern professional teacher, mentor, tutor, who can provide significant assistance in self-determination and adaptation to modern society of each student. Discussion and Conclusions. The article presents the results of the activity of the "Mentoring School" operating on the basis of the MAOU "School No. 187" of Nizhny Novgorod. Special attention in the activities of the "School of Mentoring" was paid to rethinking the system of methodological work, improving the research and methodological culture of the teacher, developingand implementing methodological support for generalizing the experience of mentoring, which ultimately contributes to the self-development of the teacher's personality, his pedagogical skills and career growth.
A survey on antimicrobial resistance genes of frequently used probiotic bacteria, 1901 to 2022
Authors: Tóth Adrienn Gréta , Judge Maura Fiona , Nagy Sára Ágnes , Papp Márton , Solymosi Norbert 
Published: April 2023

Background
Antimicrobial resistance (AMR) is caused by AMR determinants, mainly genes (ARGs) in the bacterial genome. Bacteriophages, integrative mobile genetic elements (iMGEs) or plasmids can allow ARGs to be exchanged among bacteria by horizontal gene transfer (HGT). Bacteria, including bacteria with ARGs, can be found in food. Thus, it is conceivable that in the gastrointestinal tract, bacteria from the gut flora could take up ARGs from food.

Aim
The study objective was to gain insight into the ARG set carried by commonly used probiotic bacteria that may enter the human body with non-fermented foods, fermented foods, or probiotic dietary supplements (FFPs) and to assess ARG mobility.

Methods
Next generation sequencing whole genome data from 579 isolates of 12 commonly employed probiotic bacterial species were collected from a public repository. Using bioinformatical tools, ARGs were analysed and linkage with mobile genetic elements assessed.

Results
Resistance genes were found in eight bacterial species. The ratios of ARG positive/negative samples per species were: Bifidobacterium animalis (65/0), Lactiplantibacillus plantarum (18/194), Lactobacillus delbrueckii (1/40), Lactobacillus helveticus (2/64), Lactococcus lactis (74/5), Leucoconstoc mesenteroides (4/8), Levilactobacillus brevis (1/46), Streptococcus thermophilus (4/19). In 66% (112/169) of the ARG-positive samples, at least one ARG could be linked to plasmids or iMGEs. No bacteriophage-linked ARGs were found.

Conclusion
The finding of potentially mobile ARGs in probiotic strains for human consumption raises awareness of a possibility of ARG HGT in the gastrointestinal tract. In addition to existing recommendations, screening FFP bacterial strains for ARG content and mobility characteristics might be considered.
HIV Counseling and Testing Uptake, Knowledge and Attitude and Influencing Factors among Student Nurses and Midwives in The Gambia: An Institutional-based Cross-sectional Study
Authors: Mendy Sang , Bajinka Ousman , Barrow Amadou , Mei Sun 
Published: April 2023

HIV counseling and testing (HCT) has become paramount in the prevention and control of HIV/AIDS worldwide. However, the uptake of HCT has been very slow globally, especially in sub-Saharan Africa. Student nurses formed the largest group undergoing health care training in the country compared to doctors and other health cadres. According to WHO, they are part of the most vulnerable group to HIV infection, judging by the fact that they interact more with patients/clients than other healthcare professionals. This study aimed to determine the prevalence of HCT uptake, knowledge, and attitude and evaluate influencing factors among student nurses and midwives in public nursing schools. An institutional-based cross-sectional study design was employed to collect data from 305 randomly selected nursing students and midwives using a validated and reliable self-administered questionnaire. Descriptive statistics (percentages, mean and standard deviation) and inferential statistics (chi square, logistics regression, one-way ANOVA and independent samples t-test) were used for data analysis using SPSS version 25.0. A p-value < 0.05 was considered for statistical significance. Out of the 305 students recruited for the study, 60.98% were females, with a mean age of 25.5 years old. About 58.4% of the participants had tested for HIV in the past. About 95.7% acknowledged the importance of HCT in the prevention and control of HIV/AIDS. HCT uptake among student nurses and midwives was influenced by factors ranging from an individual that are interpersonal challenges (such as concerns of friends), perceived susceptibility to the disease, lack of confidentiality, stigma and discrimination from health service providers. Therefore, these barriers can be addressed through an organized targeted health education intervention and advocacy programs across health training institutions in the Gambia and beyond.
Susceptibility of various cell lines to the <i>Chikungunya virus</i> and method selection for commercial-scale production of viral material
Authors: Kaa K. V. , Ignatyev G. M. , Sinyugina A. A. , Ishmukhametov A. A. 
Published: April 2023

An increase in cases of chikungunya fever is reported in the Caribbean, Central and South America, and Southeast Asia. As there is no specific treatment for this disease and the only available treatment is symptomatic, it is very relevant to develop vaccines against chikungunya fever. To develop an inactivated whole-virion vaccine against the disease, it is important to choose a susceptible cell culture that both provides high virus yields and is used for vaccine production.The aim of the study was to evaluate the susceptibility of multiple cell lines to Chikungunya virus infection and to select the monolayer culture method with the highest virus accumulation and yield.Materials and methods. The study used the CHIKV_Nic strain of the Chikungunya virus and cell lines C6/36 (for virus titration), CEF, MRC-5, Vero, and 4647. While choosing the culture method, the authors used culture flasks, a cell factory, and roller bottles. The authors determined the susceptibility of the cell lines to viral infection by the degree of accumulation of the infectious agent in the culture fluid. The results of virus titration were calculated on day 5 on the basis of a pronounced viral cytopathic effect.Results. The Vero and 4647 cell lines demonstrated the highest susceptibility to infection and virus concentrations in the culture fluid. The СEF and MRC-5 cell lines accumulated the virus at lower concentrations. The maximum virus titres (7.10–7.75 log10 TCID50/mL) were observed in the culture fluid 48 h after infection. The optimal multiplicity of infection (MOI) ranged between 0.001 and 0.0001 MOI/cell. At 0.0001 MOI/cell, the virus accumulated in the Vero cells cultured in roller bottles on day 2, with the maximum virus titre being 8.6±0.2 log10 TCID50/mL.Conclusions. Vero cells meet the safety and stability requirements set for the production of chikungunya vaccines. The study determined the minimum MOI of the Chikungunya virus for cell culture. The roller bottle culture method provides the highest cell culture yield and the highest titre of the virus in the culture fluid.
The association between bone mineral density and proximal junctional kyphosis in adult spinal deformity: a systematic review and meta-analysis
Authors: Chen Jeffrey W. , McCandless Martin G. , Bhandarkar Archis R. , Flanigan Patrick M. , Lakomkin Nikita , Mikula Anthony L. , Michalopoulos Giorgos D. , Bydon Mohamad 
Published: April 2023

OBJECTIVE
Proximal junctional kyphosis (PJK) is a complication of surgical management for adult spinal deformity (ASD) with a multifactorial etiology. Many risk factors are controversial, and their relative importance is not fully understood. The authors aimed to elucidate the association between bone mineral density (BMD) and PJK.

METHODS
A systematic literature search was performed using PubMed and Web of Science keywords of "Proximal Junctional Kyphosis [MeSH] OR Proximal Junctional Failure [MeSH]" AND "Bone Mineral Density [MeSH] OR Hounsfield Units [MeSH] OR DEXA [MeSH]" set to the date range of January 2002 to July 2022. Studies required a minimum of 10 patients and 12 months of follow-up. Articles were included if they were in the English language and presented a primary retrospective cohort that included a comparison of patients with and without PJK, as well as a radiographic biomarker for BMD, such as Hounsfield units (HU) or T-score.

RESULTS
A total of 18 unique studies with 2185 patients who underwent ASD surgery were identified. Of these, 537 patients (24.6%) developed PJK. Eight studies provided T-scores that were amenable to comparison, which found that patients who developed PJK were found to have lower BMD T-scores by a mean of −0.69 (95% CI −0.88 to −0.50; I2 = 63.9%, p < 0.001). The HU at the UIV among patients with the PJK group (n = 101) compared with the non-PJK group (n = 156) was found to be significantly lower (mean difference −32.35, 95% CI −46.05 to −18.65; I2 = 28.7%, p < 0.001).

CONCLUSIONS
This meta-analysis suggests that low preoperative BMD as measured by T-score and a diagnosis of osteoporosis were associated with higher postoperative PJK. Additionally, lower HU on CT at the UIV were found to be significant risk factors for postoperative PJK as well. These findings suggest that more attention to preoperative BMD is a risk factor for PJK among ASD patients is warranted.
Improvement of pulmonary function and reconstructed 3D lung volume after deformity correction for thoracic spinal posttubercular kyphosis: a multicenter study
Authors: Lin Sijian , Ma Shengbiao , Zhou Zhenhai , Ge Zhaohui , Wang Yingsong , Yang Cao , Huang Zhongren , Cao Kai 
Published: April 2023

OBJECTIVE
The aim of this study was to investigate the influence of corrective surgery on thoracic spinal posttubercular kyphosis (PTK) with respect to lung volume and pulmonary function.

METHODS
This was a retrospective study of 126 patients (72 males and 54 females) who underwent posterior vertebral column resection (PVCR) for severe thoracic spinal PTK between September 2013 and June 2020. The patients’ spinal parameters, results of their pulmonary function test (PFT), and CT-based 3D lung volume were recorded and analyzed preoperatively and at final follow-up. The correlation of kyphosis correction with the PFT and lung volume was evaluated.

RESULTS
The mean local kyphosis decreased from 112.5° to 37.2°, and the mean local scoliosis decreased from 20.9° to 5.2°; C2–7 lordosis, thoracic kyphosis, and lumbar lordosis also significantly improved after surgery. The mean CT-based lung volume significantly increased from 2.9 L preoperatively to 3.6 L at the final follow-up. The indices of PFT, including forced vital capacity (FVC), percent predicted FVC, total lung capacity, and forced expiratory volume in 1 second, were also significantly improved, and 60 patients with pulmonary dysfunction recovered to normal at the final follow-up. The correlation analysis revealed that the correction of local kyphosis was closely correlated with the improvement in PFT and the increase in lung volume.

CONCLUSIONS
PVCR cannot only effectively realign the spine in patients with severe thoracic spinal PTK deformity but also significantly improve pulmonary function. Adequate local kyphosis correction should be highly valued, as it is a key factor in increasing lung volume.
Clinical features and therapeutic outcome of 30 patients diagnosed with primary myelofibrosis at the national center of hematology
Authors: Alwan Alaa F. 
Published: April 2023

Background: Primary myelofibrosis is characterized by clonal expansion of hematopoietic stem cell with a non-reactive clonal proliferation of fibroblasts and bone marrow fibrosis, which occurs at an extramedullary hematopoiesis. The clinical features of Primary myelofibrosis include anemia, marked splenomegaly and constitutional symptoms. Ineffective erythropoiesis and extra-medullary hematopoiesis are the main causes of anemia and organomegaly, respectively Objectives : the aim of this study was to evaluate the clinical features, diagnostic tools and the treatment outcome of patients with primary myelofibrosis.Patients and methods: this is a prospective study conducted at the national center of hematology in Baghdad during the period between October 2009 and October 2013. It includes 30 adult patients diagnosed with primary myelofibrosis. Diagnosis was based primarily on bone marrow aspirate and biopsy. The patients were followed up and assessed monthly with complete blood picture and liver function test and renal function test with serum lactate dehydrogenase level.Results: thirty patients were included in this study. There were 16 male and 14 female, the median age was 58 year with range (26-75) years. The most frequent presentation was in the group older than 60 years. Twenty four (80 %) patients had symptoms at diagnosis. The most frequent presentation were the fatigue, left upper quadrant swelling, night sweats, weight loss and fever. The main clinical features were huge splenomegaly which is found in 29 (97%) of patients, while the main finding in blood count at presentation were anemia followed by leukocytosis with leukoerythroblastic blood picture in 22 (73 %) of cases. The level of LDH was high in about 23 (77 %). The bone marrow biopsy showed fibrosis stage 2 in 24 (80 %).Patients who died were in the highest risk groups. The median survival was approximately 28 months.Conclusion: Primary myelofibrosis is part of myeloproliferative neoplasia; it usually runs chronic course. Till now none of the therapeutic modalities approved for treatment of primary myelofibrosis proved to be superior to others, yet the 5 year survival of this disease considered good.
Full-Length SMN Transcript in Extracellular Vesicles as Biomarker in Individuals with Spinal Muscular Atrophy Type 2 Treated with Nusinersen
Authors: Trifunov Selena , Natera-de Benito Daniel , Carrera-García Laura , Codina Anna , Expósito-Escudero Jesica , Ortez Carlos , Medina Julita , Torres Alcala Soraya , Bernal Sara , Alias Laura , Badosa Carmen , Balsells Sol , Alcolea Daniel , Nascimento Andres , Jimenez-Mallebrera Cecilia 
Published: April 2023

Background: Three therapeutic strategies have radically changed the therapeutic scenario for spinal muscular atrophy (SMA). However, therapeutic response differs between individuals. There is a need to identify biomarkers to further assess therapeutic response and to better understand which variables determine the extent of response. Methods: We conducted a study using an optimized digital droplet PCR-based method for the ultra-sensitive detection of SMN transcript in serum EVs from SMA 2 individuals treated with nusinersen over 14 months. In parallel, we investigated levels of serum and CSF neurofilament heavy chain (pNF-H) in the same cohort. Results: Expression of flSMN transcript in EVs of SMA 2 individuals prior to nusinersen was lower than in controls (0.40 vs 2.79 copies/ul; p < 0.05) and increased after 14 months of nusinersen (0.40 vs 1.11 copies/ul; p < 0.05). The increase in flSMN with nusinersen was significantly higher in younger individuals ( p < 0.05). Serum pNF-h was higher in non-treated individuals with SMA 2 than in controls (230.72 vs 22.88 pg/ml; p < 0.05) and decreased with nusinersen (45.72 pg/ml at 6 months, 39.02 pg/ml at 14 months). CSF pNF-h in SMA 2 individuals also decreased with nusinersen (248.04 pg/ml prior to treatment, 197.10 pg/dl at 2 months, 104.43 pg/dl at 6 months, 131.03 pg/dl at 14 months). Conclusions: We identified an increase of flSMN transcript in serum EVs of SMA 2 individuals treated with nusinersen that was more pronounced in the younger individuals. Our results indicate that flSMN transcript expression in serum EVs is a possible biomarker in SMA to predict or monitor the response to treatment.
INFLUENCES OF FEED AND SEASON ON BREED AND REPRODUCTIVE TRAITS IN WEST AFRICAN DWARF AND SAHEL BUCKS IN TARABA STATE
Authors: WABA Y.E. , UMARU M. G. , MOHAMMED A.U 
Published: April 2023

The study evaluated the influence of feed on the reproductive parameters of West African Dwarf and Sahel goats at theTeaching and Research farm of the Federal Polytechnic Bali, Taraba State. A total of 8 bucks from two goats breeds (West African Dwarf and Sahel) were used for the experiment. The 8 bucks were later moved to Vom, for semen collection, using electro-ejaculator. The study lasted for 12 calendar months. They were fed Gmelina and cassava peel meal, Gmelina and cowpea husk, Ficus and cassava peel meal and, Ficus and cowpea husk as experimental treatments (T1-T4), respectively. Goats according to breed were allotted to treatments randomly and treated as factorial experiments. Season had significant (P<0.01) influence on buck parameters (scrotal length and semen qualities). The overall samples collected was 192 on both the breeds on the following parameters; scrotal circumference, semen volume, semen pH, sperm concentration, sperm motility and live cells and the results were 11.91cm, 0.47ml, 6.78ph, 414.92x106, 83.02% and 92.33% respectively. The result was significant for breed, season and treatment for all parameters except live and dead cells for breeds and treatment. Feeding Ficus containing diets resulted in higher semen quality values. Also, semen colour using likert scale was analyzed which resulted to three types of colour (creamy, milky and watery). The colour was evaluated under based on breed, seasons and treatment diets (feeds). The overall results show insignificant differences for all the parameters (feeds, seasons, breeds and reproductive traits) observed.
Students’ Performance in Speaking Skill and Self-Efficacy : An Correlational Study
Authors: Khotimah Khusnul , Amumpuni Rengganis Siwi , Arifin Samsul 
Published: April 2023

The objective of this study is to investigate the extent to which students' levels of self-efficacy are correlated with their levels of success in speaking skill assessments of the students of MAN 1 Madiun. The research method used quantitative research. The population in this study are all the eleventh grade students. The sample for this study are students from class XI MIA 2 consists of 22 students. The researcher uses using purposive sampling technique. The researcher uses questionnaires to students to determine the level of students’ self-efficacy and uses speaking test. Pearson Product Moment (r) formula using t-test is used in this research. The results show that the highest score is 95. Meanwhile, the lowest score is 82. The result of the Pearson product moment correlation is.052 with a negative sign. The conclusion that can be drawn from these findings is that there is a negative correlation between speaking ability and self-efficacy, as well as the results of significance levels above 0.05.This is what causes scores on students to be lacking. Besides self-efficacy, it has a very positive effect on each student. The implication of this research is to describe the correlation between students’ self-efficacy and their performance in speaking skill so the teacher and other researchers can get information related with that.
IMPLEMENTATION OF THE SMART METHOD IN THE DECISION SUPPORT SYSTEM FOR PENALTY RECOMMENDATIONS
Authors: Ginting Novita Br , Widhyaestoeti Dahlia , Jaenuddin Jejen , Rachmawati Fitria , Maolani Yusup 
Published: April 2023

Sanctions need to be given to students who do not obey school rules, and the purpose is to establish student discipline and ethics. SMK Gazza Wiguna 1 Megamendung Bogor district also imposes sanctions for students who violate the rules of conduct. The problem is that determining the violation point has not been done objectively. To overcome this, applying the Simple Multi-Attribute Rating Technique Method (SMART) method to the decision support system to determine recommendations for sanctions for students who commit violations. This system is designed to use UML tools and is built on a web basis. The system uses an alternative oral warning, first warning letter, second warning letter, third warning letter, and returned to parents (DO). The criteria include violation of school uniforms, tidiness, discipline, cleanliness, order, hospitality, commemorating national holidays, and additional provisions. The type of criteria used is the cost criteria because it is expected that students do not violate school rules; if students commit violations, it is hoped that students do not commit serious transgressions.
Application in the analysis of the occlusal force of free-end missing tooth implant restoration with T-SCAN III
Authors: Wu Ming-Le , Lai Peng-Yu , Cheong Fan , Zhou Wen-Cheng , Xu Sang-Hui , Li Hui , Shen Shan 
Published: April 2023

Introduction: The occlusal force of the teeth in the dental arch and the remaining adjacent natural teeth will change after implant restoration with a free-end missing tooth. This study intends to use the T-SCAN III scanner to collect dynamic quantitative data before and after the restoration of free-end implants and to explore the application of the T-SCAN III in redistributing the occlusal force of free-end implants.Methods: In this study, 24 patients with free-end implant restoration were selected, and their occlusion was tested before, immediately after, and 3 months after implant restoration.Results: In all 24 cases, the bite force of the first natural tooth adjacent to the implanted tooth after restoration changed from 19.12% ± 9.48%–12.93% ± 11.47% (p &lt; 0.01). For additional data analysis, all cases were further subdivided by single implant and fixed bridge restorations. In 17 cases, there was a successful follow-up after 3 months. The percentage of the total bite force of dental arch with implant increased from 41.92% ± 10.78%–53.06% ± 10.71% (p &lt; 0.01).Discussion: This study shows that the free-end implant restoration protects the remaining natural teeth, and the patient’s missing dental arch bite force improves within 3 months of implant restoration.