bluuebunny/crossref_metadata_2025_split huggingface dataset:
Comprehensive analysis of the prognosis and immune infiltrates for the BET protein family reveals the significance of BRD4 in glioblastoma multiforme
Authors: Ye Yintao , Zhong Wei , Qian Junqiang , Zhang Jie , Xu Tingting , Han Ruyi , Han Jiangeng , Wang Chunwei , Song Lichao , Zeng Xianwei , Wang Hong 
Published: January 2023

Background: Glioblastoma multiforme (GBM) is the most common and invasive primary central nervous system tumor. The prognosis after surgery, radiation and chemotherapy is very poor. Bromodomain (BRD) proteins have been identified in oncogenic rearrangements, and play a key role in the development of multiple cancers. However, the relationship between BET proteins and prognosis of GBM are still worth exploring, and the distinct functions of BET proteins and tumor immunology in GBM have not been fully elucidated. Therefore, it is particularly important to develop effective biomarkers to predict the prognosis of GBM patients.Methods: Metascape, David, Kaplan-Meier Plotter, Oncomine, GEPIA, TCGA, TIMER, and LinkedOmics databases were used to assess the expression and prognosis for BET proteins in GBM. ROC analysis of risk model was established to identify the correlation between BET genes and overall survival in GBM patients. TIMER and GEPIA databases were used to comprehensively investigate the correlation between BET genes and tumor immune infiltration cells. Moreover, the image of immunohistochemistry staining of BET proteins in normal tissue and tumor tissue were retrived from the HPA database. In addition, differential analysis and pathway enrichment analysis of BRD4 gene expression profile were also carried out. Finally, immune-fluorescence and Western blot were used to clarify the expression of BRD4 in GBM cells.Results: Bioinformatics analysis showed that the expression levels of BET genes in GBM may play an important role in oncogenesis. Specifically, bioinformatic and immunohistochemistry analysis showed that BRD4 protein was more highly expressed in tumor tissues than that in normal tissues. The high expression of BRD4 was associated with poor prognosis in GBM. The expression of BET genes were closely related to the immune checkpoint in GBM. The correlation effect of BRD4 was significantly higher than other BET genes, which represented negative correlation with immune checkpoint. The expression of BRD4 was positively associated with tumor purity, and negatively associated with immune infiltration abundance of macrophage, neutrophil and CD8+ T-cell, respectively. Cox analysis showed that the model had good survival prediction and prognosis discrimination ability. In addition, the expression levels of BRD4 protein was significantly higher in U-251 MG cells than that in normal cells, which was consistent with the results of bioinformatics data.Conclusion: This study implied that BRD4 could be hopeful prognostic biomarker in GBM. The increased expression of BRD4 may act as a molecular marker to identify GBM patients with high-risk subgroups. BRD4 may be a valuable prognostic biomarker, and a potential target of precision therapy against GBM.
Expression profiling analysis reveals key microRNA–mRNA interactions in patients with transposition of the great arteries and systemic left and right ventricles
Authors: Abu-Halima Masood , Wagner Viktoria , Rishik Shusruto , Raedle-Hurst Tanja , Meese Eckart , Abdul-Khaliq Hashim 
Published: January 2023

BackgroundPatients with transposition of the great arteries (TGA) have different connected systemic chambers and this determines the long-term morbidities and survival. Limited findings have been reported to systematically identify miRNA and mRNA expression levels in such cohorts of patients. In this study, we aimed to characterize miRNAs, mRNAs, and miRNA–mRNA interaction networks in patients with TGA, with a systemic left (LV) and right ventricle (RV).Materials and methodsLarge panel of human miRNA and mRNA microarrays were conducted to determine the genome-wide expression profiles in the blood of 16 TGA-RV patients, 16 TGA-LV patients, and 16 age and gender-matched controls. Using real-time quantitative PCR (RT-qPCR), the differential expression level of a single miRNA was validated. Enrichment analyses of altered miRNA and mRNA expression levels were identified using bioinformatics tools.ResultsAltered miRNA and mRNA expression levels were observed between TGA-RV and TGA-LV patients, together or separated, compared to controls. Among the deregulated miRNAs and mRNAs, 39 and 101 miRNAs were identified as significantly differentially expressed in patients with TGA (both TGA-RV and TGA-LV) and TGA-RV, when compared to matched controls. Furthermore, 51 miRNAs were identified as significantly differentially expressed in patients with TGA-RV when compared to patients with TGA-LV. RT-qPCR relative expression level was highly consistent with microarray analysis results. Similarly, 36 and 164 mRNAs were identified as significantly differentially expressed in patients with TGA (both TGA-RV and TGA-LV) and TGA-RV, when compared to matched controls. Additionally, miR-140-3p showed a higher expression level in patients with overt heart failure (FC = 1.54; P = 0.001) and miR-502-3p showed a higher expression level in patients died due to cardiac death (FC = 1.41; P = 0.011). Integrative analysis resulted in 21 and 23 target genes with higher and lower expression levels, respectively (r ≥ 0.50 and P &lt; 0.05). These target genes (i.e., 21 and 23 target genes) showed an inverse direction of regulation with miRNA and exhibited a miRNA binding site position within the 3′UTR of the target gene.ConclusionOur findings provide new insights into a potential molecular biomarker(s) for patients with TGA that may guide better risk stratification and the development of novel targeting therapies. Future studies are needed to investigate the potential significance of miRNAs and mRNAs in TGA-related cardiovascular diseases.
Association between radiotherapy and risk of death from cardiovascular diseases in lung and bronchus cancer
Authors: Yi Zhong , Zhang Yu , Wang Yu , Gao Yun , Wang Yanhong , Li Xiangnan , Ru Songwei , Guo Na , Qiu Jingxuan , Zhang Meng 
Published: January 2023

BackgroundRadiotherapy plays an important role in the treatment of lung cancer. However, radiation-related deaths from cardiovascular disease (CVD) are a concern in these patients, and few studies have examined CVD-related death associated with lung cancer. We aimed to evaluate the risk of CVD-related death after radiotherapy in patients with lung and bronchus cancer.MethodsData were extracted from the surveillance, epidemiology, and end results database. Propensity score matching (PSM) was applied to reduce possible bias between patients who received radiotherapy and those who did not. The Kaplan–Meier method was used to estimate cardiovascular-specific survival (CVSS), and the log-rank test was used to compare CVSS between the radiotherapy and no radiotherapy groups. Cox proportional hazards regression analysis was performed to estimate the hazard ratio (HR) of CVD-related death.ResultsA total of 225,570 patients with lung and bronchus cancer were included, and 201,282 patients remained after PSM. Radiotherapy was identified as an independent risk factor for CVSS among patients with lung and bronchus cancer before PSM (HR: 1.18, P &lt; 0.001) and after PSM (HR: 1.18, P &lt; 0.001). Patients treated with radiotherapy had a significantly worse CVSS than those who did not receive radiotherapy before PSM (25-year CVSS: 49.9 vs. 56.4%, P = 0.002) and after PSM (25-year CVSS: 48.4 vs. 56.7%, P &lt; 0.001). Radiotherapy was associated with more deaths from heart disease before PSM (81.9 vs. 77.2%, P &lt; 0.001) and after PSM (83.0 vs. 78.7%, P &lt; 0.001).ConclusionRadiotherapy is associated with an increased risk of CVD-related death, especially death from heart disease, in patients with lung and bronchus cancer. More efforts are needed to monitor cardiovascular health after radiotherapy.
The value of HDL subfractions in predicting cardiovascular outcomes in untreated, diabetic patients with stable coronary artery disease: An age- and gender-matched case-control study
Authors: Zhang Wei , Jin Jing-Lu , Zhang Hui-Wen , Zhu Ya-Xin , Dong Qian , Sun Jing , Guo Yuan-Lin , Dou Ke-Fei , Xu Rui-Xia , Li Jian-Jun 
Published: January 2023

ObjectiveThe aim of the present study was to examine the value of high-density lipoprotein (HDL) subfractions for predicting cardiovascular events (CVEs) in untreated type 2 diabetes mellitus (T2DM) patients with stable coronary artery disease (SCAD) using an age- and gender-matched case-control study.MethodsIn total, 185 SCAD patients and 185 T2DM patients with SCAD were enrolled and subjected to a clinical follow-up of CVEs. HDL subfractions were analyzed using the Quantimetrix Lipoprint System. The relationship between HDL subfractions and CVEs in T2DM patients with SCAD was evaluated by Kaplan–Meier analysis and Cox proportional hazard models.ResultsDuring a median 37.7-month follow-up, T2DM patients with SCAD had a higher percentage of CVEs compared to SCAD patients (p=0.039). The concentration of the combined intermediate and small HDL-C subfraction (defined as the mixed HDL subfraction) was related to the event incidence in T2DM patients with SCAD (p=0.004), and it was positively associated with increased CVEs even after adjustment in three models. Kaplan-Meier curve analysis indicated that T2DM patients with SCAD in the high mixed HDL subfraction group (&gt;28 mg/dL) had lower event-free survival rates (p=0.008).ConclusionsElevated concentration of the mixed HDL subfraction predicts events in T2DM patients with SCAD.
Decarbonization roadmap for China’s energy sector in the context of carbon futures trading: An energy-environment-economy assessment perspective
Authors: Li Jidong , Li Xiaojing , Wei Chao , Deng Jian , Chen Shuangyin , Cao Yuwei , Chen Yingqi 
Published: January 2023

By virtue of the hedging and price discovery function, carbon futures trading may help carbon market function more effectively. Is it necessary to establish carbon futures trading in China? The authorities have endorsed the idea since 2015, however, the scheme has not yet started; no antecedent pre-assessment quantitative research has been carried out. Therefore this study that attempts to fill this gap in the literature, could be of real significance. Through deriving a potential decarbonization roadmap, this study tries to give some clues pertaining to the converted mitigation strategy imposed by carbon futures trading in China. A model chain has been proposed, which is composed of the Optimal Production Decision-making Model for Producers, Life Cycle Impact Assessment, Monetization, and Genetic Algorithm based optimization, to quantify the environmental benefits (including improvements to human health, ecosystem damage and increased temperature induced GDP losses) of the assumed mitigation trajectories. By setting the maximization of environmental benefits as the objective, the optimal decarbonization roadmap with carbon futures trading is derived. Results show that the optimal emission reductions for power enterprises (covered by the carbon market) for the next 10 years (2021–2030) are around 3.27 billion tonnes CO2e. If 36% of this amount is assigned to previously discussed mitigation trajectories, it is found that 106.98 GW ultra-super critical units, 160.85 GW mono-Si PV facilities and 167.26 GW doubly-fed induction generator wind capacity should be installed. Overall environmental benefits are 4.6 trillion CNY2018, over 5% of China’s 2018 GDP. Results demonstrate the optimal emission reductions and potential decarbonization roadmap for China’s power enterprises (those covered by the carbon market) under the context of carbon futures trading, which can be an important reference for the authorities and therefore encourage the establishment of the scheme.
Clinical characteristics and long-term outcome of patients with
gastrointestinal involvement in eosinophilic granulomatosis with polyangiitis
Authors: Li Rongli , Chen Yingying , Zhang Shangzhu , Peng Linyi , Zhou Jiaxin , Fei Yunyun , Zhang Wen , Zhao Yan , Zeng Xiaofeng 
Published: January 2023

ObjectiveThis study aims to investigate clinical characteristics, potential risk factors, as well as long-term outcome in EGPA patients with GI involvement.MethodsA total of 94 EGPA patients were included in this cohort study. We retrospectively reviewed the clinical data, treatment, and outcome of 21 EGPA patients with GI involvement and compared them with other 73 EGPA patients without GI involvement. Multivariate logistic regression was used to find potential risk factors associated with GI involvement in EGPA patients.ResultsCompared with EGPA patients without GI involvement, EGPA patients with GI involvement had higher level of hs-CRP (65.1 (24.5-138.9) vs. 21.3 (5.7-39.1) mg/L, p=0.005), higher grades of Birmingham vasculitis activity score (BVAS) (20 (13-29.5) vs. 12 (16-19), p=0.022), higher Five Factor Score (FFS) (1 (1-2) vs. 0 (0-1), p&lt;0.001), and were more likely to have weight loss (66.7% vs. 38.4%, p=0.021) at baseline. In EGPA patients with GI involvement, the most common gastrointestinal symptoms were abdominal pain (90.5%) and diarrhea (42.9%). Weight loss was identified as a potential risk factor for GI involvement in EGPA patients (OR = 4.304, 95% CI 1.339–13.841). During follow-up, EGPA patients with GI involvement showed lower 1-year cumulative survival rate (75.2% vs. 100.0%, P &lt;0.0001) and 3-year cumulative survival rate (67.7% vs. 100.0%, P&lt;0.0001), lower long-term remission rate (33.3% vs. 86.3%, P&lt;0.001), but higher 1-year cumulative relapse rate (19.2% vs. 3.8%, P=0.03) and 3-year cumulative relapse rate (54.6% vs. 13.1%, P&lt;0.001) compared with patients without GI involvement.ConclusionEGPA patients with GI involvement had distinct features from those without GI involvement, including higher hs-CRP level, higher BVAS and FFS scores. EGPA patients with GI involvement showed lower cumulative survival rate, lower long-term remission rate and higher cumulative relapse rate compared with those without GI involvement.
Effectiveness and safety of anakinra in gouty arthritis: A case series and review of the literature
Authors: Jeria-Navarro Sicylle , Gomez-Gomez Alejandro , Park Hye Sang , Calvo-Aranda Enrique , Corominas Hector , Pou Maria Antonia , Diaz-Torne Cesar 
Published: January 2023

BackgroundGout is the most common type of inflammatory arthritis. Nonsteroidal anti-inflammatory drugs, corticosteroids, and colchicine are the first-line agents, although they are contraindicated in many patients. Blockade of IL-1 with anakinra can be an alternative.ObjectiveTo present a case series of 10 difficult-to-treat gout patients treated with anakinra and perform a scoping review of the effectiveness and safety of anakinra in gout patients.MethodsA total of 1,519 citations were screened. The reviewers ran a two-stage screening process by title/abstract and full-text reading. Thirty-eight articles finally met the selection criteria and were included for data extraction and synthesis. Experience in difficult-to treat and complex clinical scenarios, such as active infection, hemodialysis, and transplantation, were specifically described.ResultsThe study sample comprised 551 patients, from whom 648 flares were finally analyzed. The mean age was 57.9 years, and 82.9% were men. The clinical presentation was polyarticular in 47.5% and tophaceous in 66.9%. Sixty-five patients with an active infection, 41 transplanted patients and 14 in haemodyalisis treated with anakinra are described. More than half of the patients had &gt;1 associated comorbidity. Anakinra was effective both for flares (94%) and for long-term treatment (91%) and well tolerated. In the case of flares, 34 (6.7%) adverse effects were registered. Adverse events were more prevalent in long-term treatment.ConclusionAnakinra was effective and safe for management of gout flares in difficult-to-treat patients. It has been used in multiple complex scenarios, such as active infections, dialysis, transplantation, chronic kidney disease, and polyarticular gout. Anakinra has also proven effective as long-term treatment, although there are more concerns about its safety.
A spiking network model for clustering report in a visual working memory task
Authors: Lei Lixing , Zhang Mengya , Li Tingyu , Dong Yelin , Wang Da-Hui 
Published: January 2023

IntroductionWorking memory (WM) plays a key role in many cognitive processes, and great interest has been attracted by WM for many decades. Recently, it has been observed that the reports of the memorized color sampled from a uniform distribution are clustered, and the report error for the stimulus follows a Gaussian distribution.MethodsBased on the well-established ring model for visuospatial WM, we constructed a spiking network model with heterogeneous connectivity and embedded short-term plasticity (STP) to investigate the neurodynamic mechanisms behind this interesting phenomenon.ResultsAs a result, our model reproduced the clustering report given stimuli sampled from a uniform distribution and the error of the report following a Gaussian distribution. Perturbation studies showed that the heterogeneity of connectivity and STP are necessary to explain experimental observations.ConclusionOur model provides a new perspective on the phenomenon of visual WM in experiments.
What is the level of nutrition literacy of Saudi adolescents? A national wide exploratory cross-sectional study
Authors: Bookari Khlood 
Published: January 2023

BackgroundDespite being a prolific research topic, studies evaluating nutrition literacy in the Arab world are scant and still nonexistent in the Kingdom of Saudi Arabia. Therefore, a national study was launched with the aim to (1) assess nutrition literacy among Saudi adolescents aged 10–19 years old and (2) determine its correlates.MethodsA cross-sectional study was launched across all Saudi provinces between 29 April and 6 June 2022, enrolling a convenience sample of 2,115 adolescents (mean age = 16.8 ± 2.5; girls: 48.7%). An online self-administered questionnaire was disseminated to eligible participants to collect relevant data.ResultsStudy findings showed that nearly half of the adolescents (44.6%) had poor nutrition literacy. The male gender doubled the risk of adolescents having poor nutrition literacy (OR = 2.0, CI = 1.5-2.5, p &lt; 0.001). Northern border residents were 14 times more likely to be nutritionally illiterate, in contrast to adolescents residing in Riyadh (OR = 14.0, CI = 7.3–28.0, p &lt; 0.001). Adolescents were more likely to be nutritionally illiterate if they were underweight or overweight (OR = 2.7, CI = 1.6–4.7, p &lt; 0.001; OR = 2.2, CI = 1.7–2.9, p &lt; 0.001). School students had 2 times more risk of having poor nutrition literacy, in contrast to those who were enrolled in universities (OR = 1.8, CI = 1.4–2.4, p &lt; 0.001). Nutrition illiteracy was 4 times higher among adolescents having caregivers else than their parents (OR = 3.9, CI = 2.2–6.9, p &lt; 0.001). Parental education level also contributed to determining adolescents' nutrition literacy status.ConclusionsIt has become essential to courage the development of supportive school environments in Saudi Arabia to promote nutrition education and improve adolescents' nutrition literacy. Without a doubt, this calls for taking a holistic approach on the part of education and health ministries, nutrition professionals, educators, parents, and, most importantly, the teenagers themselves, who must have the desire and motivation to learn.
Research trends and hotspots of neurodegenerative diseases employing network pharmacology: A bibliometric analysis
Authors: Zhu Jie , Liang Qingchun , He Siyi , Wang Chen , Lin Xiafei , Wu Duozhi , Lin Guanwen , Wang Zhihua 
Published: January 2023

Background: Employing network pharmacology in neurodegenerative diseases (NDs) has been extensively studied recently. However, no comprehensive study has conducted on this subject employing bibliometrics so far. The purpose of this study was to find out the developmental trends and hotspots, and to predict potential research directions in this filed.Methods: Relevant research were collected from the Web of Science Core Collection Bibliometrics and visual analysis were executed using CiteSpace, VOSviewer, Histcite and R-bibliometrix.Results: A total of 420 English articles on network pharmacology in NDs published in 2008–2022 were obtained from the WOSCC database. From 2008 to 2022, annual publications showed a steady growing trend, especially in 2014–2022. China, Beijing Univ Chinese Med, Frontiers in Pharmacology, and Geerts H are the most prolific country, institution, journal, and author, respectively. China, Nucleic Acids Research, and Hopkins AL are the most highly cited country, journal, and author, respectively. Moreover, network pharmacology and Alzheimer’s disease are the focal areas of current researches according to analysis of co-cited references and keywords. Finally, in the detection of burst keywords, systems pharmacology and database are new approaches to disease and drug research, while traditional Chinese medicine (TCM) and Alzheimer’s disease are hot research directions. The above keywords are speculated to be the research frontiers.Conclusion: Network pharmacology and Alzheimers’ disease are the main topics of researches on network pharmacology in NDs. Network pharmacology and the TCM treatment of Alzheimer’s disease have been the recent research hotspots. To sum up, the potential for exploring TCM treatment of AD with network pharmacology is huge.
Piper sarmentosum Roxb. methanolic extract prevents stress-induced gastric ulcer by modulating oxidative stress and inflammation
Authors: Akmal Muhamad Nurul , Abdel Aziz Ibrahim , Nur Azlina Mohd Fahami 
Published: January 2023

This study investigated the gastroprotective effect of Piper sarmentosum (PS) on stress-induced gastric ulcers in rats by measuring its effect on oxidative stress, gastric mucosal nitric oxide (NO), and inflammatory biomarkers. Twenty-eight male Wistar rats were randomly divided into four groups; two control groups (non-stress and stress) and two treated groups supplemented with either methanolic PS extract (500 mg/kg body weight) or omeprazole (OMZ; 20 mg/kg) orally. After 28 days of treatment, the stress control, PS, and OMZ groups were subjected to water-immersion restrain stress (WIRS) for 3.5 h. Gastric tissue malondialdehyde (MDA), NO, superoxide dismutase (SOD), inducible NO synthase (iNOS), SOD mRNA, tumor necrosis factor (TNF)-α, interleukin (IL)-1β, and IL-6 levels were measured. WIRS significantly increased gastric MDA, NO, and pro-inflammatory cytokine levels compared to the non-stressed control group. PS and omeprazole supplementation significantly reduced WIRS-exposure-induced gastric ulcers and MDA, iNOS, and IL-1β levels. However, only PS reduced NO, TNF-α, and IL-6 levels, which were upregulated in this ulcer model. In conclusion, the gastroprotection afforded by PS is possibly mediated by gastric mucosal NO normalization through reduced iNOS expression and attenuation of inflammatory cytokines. PS showed a greater protective effect than omeprazole in reducing gastric lesions and NO, TNF-α, and IL-6 levels, and iNOS expression.