bluuebunny/crossref_metadata_2025_split huggingface dataset:
Assessing the Inter-rater Reliability of the Usability Model for Software Development Processes and Practices
Authors: Fontdevila Diego , Genero Marcela , Paez Nicolás , Oliveros Alejandro 
Published: July 2023

Abstract
Software processes and practices have a leading role in software development and in the last few decades a wide variety of processes and practices have emerged to face the challenges arising in the software industry. The success of process and practice adoption initiatives depends at least partially on the experience and satisfaction of the people who use them, thus making usability an interesting process quality attribute. This paper describes the inter-rater reliability evaluations performed on the UMP to assess consistency among metric values produced by different evaluators. It presents two inter-rater reliability assessment studies, the Scrum study and the TDD-BDD study. The paper presents four inter-rater reliability statistics for the process and practices under study, a comparative analysis of their strengths and weaknesses, and an analysis of the study results and their interpretation. The results show varying reliability results among the different UMP metrics, which seem to depend mostly on object of evaluation (Scrum, TDD, BDD), metric subjectivity and context sensitivity. The UMP was refined from the data gathered in the studies and a metric categorization is proposed to facilitate metric usage.
Limited Intercarpal Fusion vs. Proximal Row Carpectomy in the treatment of SLAC or SNAC Wrist, results after 3.5 years.
Authors: Gvozdenovic Robert , Schioedt Martina Agerskov , Solgaard Lars , Vadstrup Lars Soelberg , Soee Niels Henrik 
Published: July 2023

Abstract
The present study compares the postoperative clinical, radiological, and patient-reported functional results between the surgical procedures Proximal Row Carpectomy and Limited Carpal Fusion, in the treatment of SLAC and SNAC conditions of the wrist. 15 Proximal Row Carpectomy patients and 45 Limited Carpal Fusion patients were included in the study. Postoperative outcomes were assessed and compared for pain at load, range of motion, grip strength, Quick-DASH, and satisfaction. A radiological assessment was performed at the follow-up.
The Proximal Row Carpectomy patients had a mean age of 60 years (range 31-77) and a mean follow-up of 42 months. The Limited Carpal Fusion patients had a mean age of 58 years (range 35-76) and a mean follow-up of 41 months. The patients treated with Limited Carpal Fusion performed significantly better regarding pain, radial-ulnar motion, and the Quick-DASH (p = 0.002, p = 0.003, and p= 0.002), respectively. The grip strength difference between the treatment groups was stratified for gender and was found significantly better for men in the LCF-treated patients, but not different for women (p = 0.03, p = 0.26), respectively. Differences in volar-dorsal motion between the groups were insignificant (p = 0.525). A higher conversion rate to total wrist fusion was observed in the patients treated with the Proximal Row Carpectomy. All the Proximal Row Carpectomy patients had osteoarthritis at follow-up, whereas it was seen in 19% of the Limited Carpal Fusion patients. The patient-reported satisfaction was substantially better for the Limited Carpal Fusion patients. In conclusion, besides the findings of the grip strength which was most likely affected by different gender dispersion between treatment groups, the findings are in favour of Limited Carpal Fusion compared to Proximal Row Carpectomy, among patients treated for SNAC and SLAC wrist conditions. Further, preferably prospective studies are needed to confirm or reject our findings.
Development of UME CRM 1008: Certified Reference Material for C - reactive protein
Authors: Oztug Merve , Saban Evren , Kucuk Meltem , Isleyen Alper , Akgoz Muslum 
Published: July 2023

Abstract
A certified reference material (CRM) specifically designed for C-reactive protein (CRP) measurement in clinical laboratories was produced in this study. CRP, a protein synthesized by the liver, serves as an indicator of inflammation when present in elevated concentrations in the bloodstream. The primary goal is to ensure accurate measurement and quality control in accredited clinical laboratories. To achieve this, a recombinant CRP solution was selected as the most suitable candidate material for the CRM due to its exceptional purity. The homogeneity and stability of the developed CRM were thoroughly examined using size exclusion chromatography high performance liquid chromatography (SEC-HPLC). Additionally, amino acid analysis coupled with isotope dilution mass spectrometry (ID-LC/MS-AAA) was employed to determine the precise protein concentration of CRP in the CRM solution. The evaluation of uncertainties originating from factors such as characterization, homogeneity, long-term stability, and short-term stability data was incorporated to assess the uncertainty associated with the certified CRP value. Consequently, the certified value of the CRM, denoted as UME CRM 1008, was determined to be 43.2 ± 2.2 µmol kg-1, with an expanded uncertainty at a coverage factor of k = 2. The resulting CRP-certified reference material from this study will serve as an essential standard for validating CRP measurement methods and fulfilling quality control requirements within clinical laboratories. The ultimate aim is to make this reference material widely accessible to both national and international laboratories, thereby promoting accurate and standardized CRP measurements across the world.
Incorporation of nano-hydroxyapatite into experimental resin infiltrant and its performance on color stability and reinforcement in demineralized enamel: in vitro study
Authors: Zago Jade Laísa Gordilio , de Cerqueira Gabriela Alves , de Souza Robson Ferreira , Aguiar Flávio Henrique Baggio , Tabchoury Cínthia Pereira Machado , Marchi Giselle Maria 
Published: July 2023

Abstract
Objective The aim of the study was to evaluate the influence of the incorporation of 10% nano-hydroxyapatite into an experimental resin infiltranton color stability and cross-sectional microhardness.Material and methods 135 blocks were divided into five groups: H: healthy; MB: white spot; I: Icon®; E: experimental; EH: experimental containing 10% nano-hydroxyapatite. For color evaluation (n = 15), CIEL*a*b* values were obtained at the following time points: T0 (before immersion), T1 (14 days after immersion), and T2 (28 days after). Data were applied to CIEDE2000 formula. Cross-sectional microhardness (n = 12) data from the MB, I, E, and EH groups were applied to the mineral loss formula (∆S). Polarized Light Optical Microscopy images were obtained (n = 5) at 40x magnification. Shapiro-Wilk test was used to assess data normality for color stability and mineral loss. One-way ANOVA analysis was performed, followed by Bonferroni's post hoc test (color stability) and Tukey's test (mineral loss).Results In color stability results, regardless of time, there was no significant difference between H and MB groups; at 14 days and at 28 days, MB differed from all infiltrated groups, and H differed from E and EH. For ∆S, MB group showed a significant difference compared to I and EH groups but did not differ from E.Conclusion E and EH showcased similar performance to I regarding color variation. In terms of ∆S, I and EH had less mineral loss, suggesting a reinforcement of the dental structure.Clinical Relevance: Predict color stability and structural reinforcement of resinous infiltrants applied to white spot lesions.
Concurrent inhibition of oncogenic and wild-type RAS-GTP for cancer therapy
Authors: Singh Mallika , Holderfield Matthew , Lee Bianca , Jiang Jingjing , Tomlinson Aidan , Seamon Kyle , Mira Alessia , Patrucco Enricho , Goodhart Grace , Dilly Julien , Gindin Yevgeniy , Dinglasan Nuntana , Wang Ying , Lai Lick Pui , Cai Shurui , Jiang Lingyan , Yang Yu , Nasholm Nicole , Evans James , Montazer Nilufar , Lai Oliver , Shi Jade , Ahler Ethan , Chang Stephanie , Salvador Anthony , Marquez Abby , Cregg Jim , Liu Yang , Milin Anthony , Chen Anqi , Ziv Tamar Bar , Parsons Dylan , Knox John , Roth Jennifer , Rees Matthew , Ronan Melissa , Cuevas-Navarro Antonio , Hu Feng , Lito Piro , Santamaria David , Aguirre Andrew , Waters Andrew , Der Channing , Ambrogio Chiara , Wang Zhengping , Gill Adrian , Koltun Elena , Smith Jacqueline , Wildes David 
Published: July 2023

Abstract
RAS oncogenes (collectively NRAS, HRAS, and especially KRAS) are among the most frequently mutated genes in cancer, with common driver mutations occurring at codons 12, 13 and 611. Small molecule inhibitors of the KRASG12C oncoprotein have demonstrated clinical efficacy in patients with multiple cancer types and have led to regulatory approvals for the treatment of non-small-cell lung cancer (NSCLC)2,3. Nevertheless, KRASG12C mutations account for only ~14% of KRAS mutated cancers4 and there are no approved KRAS inhibitors for the majority of patients with tumors harboring other common RAS mutations. Here, we describe RMC-7977, a reversible, tri-complex RAS inhibitor with broad spectrum activity for both mutant and wild-type (WT) KRAS, NRAS, and HRAS variants (a RASMULTI(ON) inhibitor). Preclinically, RMC-7977 demonstrated potent activity against RAS-addicted tumors carrying various RAS genotypes, particularly cancer models with KRAS codon 12 mutations (KRASG12X). RMC-7977 led to tumor regressions and was well tolerated in diverse RAS-addicted preclinical cancer models. Additionally, RMC-7977 inhibited the growth of KRASG12C cancer models that are resistant to KRASG12C inhibitors due to restoration of RAS pathway signaling. Thus, RASMULTI(ON) inhibitors can target multiple oncogenic and WT RAS isoforms and hold the potential to treat a wide range of RAS-addicted cancers with high unmet clinical need. A related RASMULTI(ON) inhibitor, RMC-6236, is currently under clinical evaluation in patients with KRASG12X mutant solid tumors (NCT05379985).
Gut microbiota-derived 5-hydroxyindoleacetic acid from Pumpkin Polysaccharides supplementation alleviates colitis through Epac/Rap1 signaling activation
Authors: Zhang Qiao , Wang Qi , Li Xiaodong , Wu Minglan , Wu Xia , Zhao Qingwei , Li Weifen , Hu Xingjiang 
Published: July 2023

Abstract
Aims
Polysaccharides from Pumpkin (Cucurbita moschata Duchesne) (PP) have many pharmacological activities, including anti-oxidant, immune, and intestinal microbiota regulation. These activities have provided some reminders of its potential therapeutic effect on ulcerative colitis (UC), but this has not yet been confirmed. This study explores the potential modulatory roles of gut microbial metabolites (5-hydroxyindole acetic acid, 5-HIAA) after PP treatment in protecting against UC and to elucidate the underlying molecular mechanisms.
Results
The average molecular weight of PP was 3.10 × 105 Da, and PP mainly comprised Mannose, Rhamnose, Galacturonic acid, Galactosamine, Glucose, and Xylose with molar ratios of 1.58:3.51:34.54:1.00:3.25:3.02. PPs (50, 100 mg/kg) could significantly resist dextran sodium sulfate (DSS) induced UC on C57BL/6 mice by improving gut microbiota dysbiosis and intestinal mucosal barrier function. Metabolomic profiling analysis showed that PP supplementation resulted in the enrichment of 5-HIAA, which exhibited individual and synergistic anti-UC activities. The results of the transcriptome and confirmatory experiments suggested that Rap1 pathway activation may participate in the anti-UC effect of PPs. 8-pCPT-2’-O-Me-cAMP (Epac/Rap1 signaling antagonist) showed similar anti-UC activity to PP and 5-HIAA, while the effect of ESI-05 (Epac/Rap1 signaling inhibitor) was opposite. Furthermore, exogenous treatment with 5-HIAA activated the Epac/Rap1 signaling pathway both in vitro and in vivo, which may be related to elevated cAMP levels in the colon.
Conclusions
This study revealed that PP could be developed to treat UC and other diseases associated with an imbalance in the intestinal microbiota. The gut microbiota-5-HIAA-Epac/Rap1 axis plays an important role in intestinal homeostasis and may provide a novel approach for the prevention of UC by manipulating gut microbial serotonin metabolites.
Prognostic value of moderate or massive ascites in patients with advanced gastric cancer
Authors: Iwai Naoto , Ohara Tomoya , Okuda Takashi , Oka Kohei , Sakai Hiroaki , Kajiwara-Kubtota Mariko , Tsuji Toshifumi , Sakagami Junichi , Kagawa Keizo , Doi Toshifumi , Inoue Ken , Dohi Osamu , Yoshida Naohisa , Uchiyama Kazuhiko , Ishikawa Takeshi , Takagi Tomohisa , Konishi Hideyuki , Itoh Yoshito 
Published: July 2023

Abstract
Background
Advanced gastric cancer is one of highly aggressive malignancies. The available literature does not provide the prognostic value of ascites based on its degree because most clinical trials exclude patients who present with massive ascites. Therefore, this study aimed to examine whether the presence or degree of ascites has a prognostic value in patients with advanced gastric cancer in clinical settings.
Methods
Totally, 124 patients with advanced gastric cancer were recruited. The degree of ascites was assessed using computed tomography (CT) and classified as none, small, moderate, or massive. The overall survival (OS) was compared based on the presence or degree of ascites. Furthermore, the Cox proportional hazards analysis was performed to ascertain the predictors of OS.
Results
The cumulative one year, and two year OS rates in patients without ascites were 43.5%, and 20.2%, respectively, whereas those in patients with ascites were 29.1%, and 13.6%, respectively (P = 0.116). The cumulative one year, and two year OS rates in patients without moderate or massive ascites were 39.5%, and 20.9%, respectively, whereas those in patients with moderate or massive ascites were 28.0%, and 4.0%, respectively (P = 0.027). In the multivariate analysis, diffuse-type (hazard ratio [HR], 1.532; 95% confidence interval [CI], 1.002–2.343; P = 0.049), moderate or massive ascites (HR, 2.153; 95% CI, 1.301–3.564; P = 0.003), and chemotherapy (HR, 0.189; 95% CI, 0.101–0.352; P < 0.001) were significant predictive factors of OS.
Conclusion
We determined that moderate or massive ascites influenced OS of patients with advanced gastric cancer.
Metagenomics analysis reveals that carbon degradation rather than carbon fixation is dominant during animal carcass decay
Authors: Wang Xiaochen , Sun Xiaofang , Yu Qiaoling , Zhang Xiao , Shi Yu , Heděnec Petr , Ma Tengfei , Qian Han , Yang Jiawei , Li Huan 
Published: July 2023

Abstract
Background Temperature shapes microbial functional genes associated with carbon (C) cycles. However, the effects of temperature on microbial C cycle genes associated with carcass-contaminated water remains poorly understood. To fill this gap, we explored the variation of microbial carbon cycling genes in polluted water caused by animal corpse decay at five different temperature gradients (23, 26, 29, 32, and 35℃) by metagenomic sequencing.
Results Corpse decay increased the total carbon by 86.6%, but temperature rise had no significant effect. The dominant phyla of C-cycle microbes were Proteobacteria, followed by Actinobacteria and Bacteroidetes. Approximately a half of carbon-cycling genes and 37.59% of carbohydrate-active enzyme genes can be predicted by temperature, while others were not sensitive to temperature. The alpha diversity of carbon cycling genes decreased with rising temperature, and beta diversity was affected by temperature and cadaver decomposition. High temperature led to an increase of those genes encoding decomposed starch, carbohydrate esters, pectin, lignin, chitin, cellulose, oligosaccharide, debranching enzymes and hemicellulose in control group, while only carbohydrate esters decomposition increased with temperature in the corpse group. The concentration of total carbon was positively related with easily degradable carbon genes (e.g., decomposed starch), but lacked correlations with refractory carbon genes (e.g., cellulose, hemicellulose and lignin).
Conclusions We found that carbon degradation rather than carbon fixation was dominated during animal carcass decay, and microbes may prioritize use the degradable carbon, such as easily decomposed amylose. Our research finds that carcass decomposition regulates carbon cycle pathway, and provides possibility for predicting carbon cycle genes under global warming.
Exosomal microRNA-122 from HCC inhibits hepatic stellate cell fibrosis activation via AMPK signaling.
Authors: Wang Ziyu , Chen Zhaozheng , Li Ziyi , Jin Yong , Tao Qianqian , Peng Hui , Yu Qingsheng , Yang Wen , Feng Qiyu , Wang Hongyang 
Published: July 2023

Abstract
sEVs are extracellular vesicles with nanoscale bilayer membranes that deliver cell-specific proteins and nucleic acids (including mRNA and miRNA) to regulate intracellular signaling pathways. The development of hepatic fibrosis is closely related to sEV and its miRNA, which regulate the activation, proliferation, apoptosis, and migration of hepatic stellate cells. In this study, we report on the regulation of human hepatic stellate cell (HSC) LX-2 cell line by sEVs derived from serum of liver cancer patients through miR-122 and its potential signaling pathway. The effect of miR-122 on mRNA and protein expression of fibrosis markers was evaluated in human hepatic stellate cell LX-2 cell line transfected with miR-122 mimics or added serum-derived sEVs from liver cancer patients using QRT-PCR and western blot analysis. The effect of AMPK on LX-2 cell activation was validated using metformin or AMPK inhibitor. Results showed that miRNA-122 was expressed at low levels in activated LX-2 cells, but serum-derived sEVs and miR-122 mimics from liver cancer patients up-regulated miR-122 levels in activated LX-2 cells and reduced the expression of fibrosis marker proteins. The phosphorylation of AMPK decreased after activation of LX-2 cells, and the level of miR-122 was positively correlated with the phosphorylation of AMPK upon verification, suggesting that sEVs derived from serum of liver cancer patients can up-regulate miR-122 levels in LX-2 cells, change the energy status of cells, and inhibit the activation of HSC. This finding may provide an explanation for the reduced degree of fibrosis observed in HCC patients.
The biosocial correlates and predictors of emotion dysregulation in autistic adults compared to borderline personality disorder and nonclinical controls
Authors: Bemmouna Doha , Lagzouli Amine , Weiner Luisa 
Published: July 2023

Abstract
Background
Emotion dysregulation (ED) is a core symptom of borderline personality disorder (BPD), whose etiology has been attributed to biosocial factors. In autism spectrum disorder (ASD), although ED is prevalent and is associated with decreased well-being (e.g., self-harm, suicidality), it has been understudied, especially in adults. It is therefore crucial to further understand ED in autistic adults to improve its treatment. Our study investigates ED, its behavioural correlates (e.g., self-harm, suicidality) and biosocial predictors in autistic adults relative to BPD and nonclinical controls (NC).
Methods
724 participants (ASD = 154; BPD = 111; NC = 459) completed 11 self-reported questionnaires assessing ED, ASD and BPD traits, co-occurring disorders, alexithymia, emotional vulnerability and invalidating experiences (e.g., bullying, autistic camouflaging). The occurrence of ED behavioural correlates (i.e., self-harm, history of suicide attempts, and psychiatric hospitalizations) was collected. In addition, between-groups analyses, linear regressions and machine learning models (ML) were used to identify ED predictors in each group.
Results
ED and its behavioural correlates were higher in ASD compared to NC, but milder than in BPD. While gender did not predict ED scores, autistic women had increased risk factors to ED, including sexual abuse and camouflaging. Interestingly, BPD traits, emotional vulnerability, and alexithymia strongly predicted ED scores across the groups. Using ML models, sensory sensitivity and autistic camouflaging were associated with ED in ASD, and ADHD symptoms with ED in BPD.
Limitations:
ASD and BPD diagnoses were self-reported, which did not allow us to check their accuracy. Additionally, we did not explore the transactional and the moderating/mediating relationships between the different variables. Moreover, our research is cross-sectional and cannot draw conclusions regarding the direction and causality of relationships between ED and other clinical dimensions.
Conclusions
ED and its behavioural correlates are heightened in BPD compared to ASD and nonclinical controls. In the ASD group, there were no gender differences in ED, despite the heightened exposure of autistic women to ED risk factors. BPD traits, emotional vulnerability, and alexithymia are core to ED regardless of diagnosis. Although less central, sensory sensitivity and autistic camouflaging seem to be specific predictors of ED in autistic adults.
Soil Erosion risk Assessment in Moridhal watershed of Assam, India using Universal Soil Loss Equation and GIS
Authors: Bharteey Prem Kumar , Deka Bipul , Patgiri D. K. , Dutta Marami , Goswami Jonali , Saikia Rituparna 
Published: July 2023

Abstract

Soil erosion is one of the major factors affecting sustainability of agricultural production in watershed. The objective of this paper is to estimate soil loss using the universal soil loss equation (USLE) model and GIS and to suggest soil conservation practices in Moridhal watershed. Soil loss was estimated by USLE. In addition, measurements of randomly selected soil and water conservation structures were done at four physiographic units of watershed. The erodibility of the studied soils was assessed by computing various erodibility indices like clay ratio, silt clay ratio, modified clay ratio, dispersion ratio, erosion ratio and erosion index. The soil loss of watershed was varied from very slight to very severe (range 0.87–67.77 t ha-1 yr-1). Among the physiographic units, the soil loss in the upper piedmont plain area was moderately severe to very severe with a value varying from 19.9–67.8 t ha-1 yr-1. The dispersion ratio of the soils varied from 0.06 to 1.18. It was observed that 48.82 per cent of the total studied soil samples had dispersion ratio values above 0.15 which may be considered as erodible. The erosion ratio and erosion index of studied soils varied from varied from 0.01–0.55 and 0.03–0.71, respectively. Based on the study it was found that the soils in different physiographic units are suffering from varying degree of soil erosion. The upper piedmont plain areas of the watershed require management practices which could improve the erosion status and soil productivity. The results of soil loss obtained from the study could be effectively used by the planners, watershed managers and policy makers to formulate site specific conservation plans in order to minimize soil loss for sustaining productivity.