bluuebunny/crossref_metadata_2025_split huggingface dataset:
Designing a minimum data set of laboratory data for the electronic summary sheet of pediatric ward in Iran: A cross‐sectional study
Authors: Shahbakhsh Farzaneh , Khajouei Reza , Sabahi Azam , Mehdipour Yousef , Ahmadian Leila 
Published: June 2023

AbstractBackground and AimIranian hospitals are provided with hospital information systems (HISs) from different vendors, which make it hardly possible to summarize laboratory data in an consistent manner. Therefore, there is a need to design a minimum data set of laboratory data that will define standard criteria and reduce potential medical errors. The purpose of this study was to design a minimum data set (MDS) of laboratory data for an electronic summary sheet to be used in the pediatric ward of Iranian hospitals.MethodsThis study consists of three phases. In the first phase, out of 3997 medical records from the pediatric ward, 604 summary sheets were chosen as sample. The laboratory data of these sheets were examined and the recorded tests were categorized. In the second phase, based on the types of diagnosis we developed a list of tests. Then we asked the physicians of the ward to select which ones should be documented for each patient's diagnosis. In the third phase, the tests that were reported in 21%–80% of the records, and were verified by the same percentage of physicians, were evaluated by the experts' panel.ResultsIn the first phase, 10,224 laboratory data were extracted. Of these, 144 data elements reported in more than 80% of the records, and more than 80% of experts approved them to be included in the MDS for patients' summary sheet. After data elements were investigated in the experts' panel, 292 items were chosen for the final list of the data set.ConclusionsThis MDS was designed such that, if implemented in hospital information systems, it could automatically enable registering data in the summary sheet when patient's diagnosis is registered.
Global seroprevalence of Toxoplasma gondii infection among patients with mental and neurological disorders: A systematic review and meta‐analysis
Authors: Bisetegn Habtye , Debash Habtu , Ebrahim Hussen , Mahmood Naunian , Gedefie Alemu , Tilahun Mihret , Alemayehu Ermiyas , Mohammed Ousman , Feleke Daniel Getacher 
Published: June 2023

AbstractBackground and AimToxoplasmosis is the most widespread zoonotic disease that affects one‐third of the world's population, and imposes a major public health problem worldwide. This study aimed to assess the prevalence of toxoplasmosis among patients with neuropsychiatric patients.MethodsElectronic databases PubMed, Google Scholar, Web of Science, Research Gate, and Scopus were thoroughly searched from February to March 2022 to identify all relevant studies. The quality of studies was evaluated using the Newcastle−Ottawa quality scale for case‐control and cross‐sectional studies. Statistical analysis was done using STATA version 12 software. A random effect model was used to compute the global pooled seroprevalence of Toxoplasma gondii infection. Heterogeneity was quantified by using I2 value. Subgroup analysis was done, and publication bias was assessed using a funnel plot and Egger's test.ResultOf 1250 studies, 49 containing 21,093 participants and conducted in 18 countries were included. The global pooled seroprevalence of T. gondii IgG antibody was 38.27% (95% CI: 32.04−44.9) among neuropsychiatric patients and 25.31% (95% CI: 21.53−29.08) in healthy controls with substantial heterogeneity of 98.3%. The prevalence of T. gondii IgG antibody was higher in males (17.52%) than in females (12.35%) neuropsychiatric patients. The highest pooled prevalence of T. gondii IgG antibody was in Europe (57%) followed by Africa (45.25%) and Asia (43%). Time based analysis showed the highest pooled prevalence of T. gondii IgG antibody in 2012−2016 (41.16%).The global pooled seroprevalence T. gondii IgM antibody among neuropsychiatric patients and healthy controls was 6.78% (95% CI: 4.87−8.69) and 3.13% (95% CI: 2.02−4.24), respectively.ConclusionThe pooled prevalence of chronic and acute T. gondii infection among neuropsychiatric patients was 38.27% and 6.78%, respectively. This showed a high burden of toxoplasmosis among neurological and psychiatric patients and urges routine screening of those patients and providing appropriate treatment. It also indicates the need for different stakeholders to develop targeted prevention and control strategies for T. gondii infection.
Scattered‐light‐sheet microscopy with sub‐cellular resolving power
Authors: Subedi Nava R. , Stolyar Sergey , Tuson Sabrina J. , Marx Christopher J. , Vasdekis Andreas E. 
Published: June 2023

AbstractSince its first demonstration over 100 years ago, scattering‐based light‐sheet microscopy has recently re‐emerged as a key modality in label‐free tissue imaging and cellular morphometry; however, scattering‐based light‐sheet imaging with subcellular resolution remains an unmet target. This is because related approaches inevitably superimpose speckle or granular intensity modulation on to the native subcellular features. Here, we addressed this challenge by deploying a time‐averaged pseudo‐thermalized light‐sheet illumination. While this approach increased the lateral dimensions of the illumination sheet, we achieved subcellular resolving power after image deconvolution. We validated this approach by imaging cytosolic carbon depots in yeast and bacteria with increased specificity, no staining, and ultralow irradiance levels. Overall, we expect this scattering‐based light‐sheet microscopy approach will advance single, live cell imaging by conferring low‐irradiance and label‐free operation towards eradicating phototoxicity.
Analysis of the adhesive interface of dentine treated with carbodiimide and chitosan before cementation of fiberglass posts with different resin cements
Authors: Assis Helena Cristina de , Bertolini Gunther Ricardo , Sousa‐Neto Manoel Damião , Lopes‐Olhê Fabiane Carneiro 
Published: June 2023

AbstractThe objective of this study is to evaluate the effect of 1‐ethyl‐3‐(3‐dimethylaminopropyl)‐carbodiimide (EDC) and chitosan (CHI) on the adhesive interface of resin cements to root dentine. Forty‐five upper canines were sectioned, endodontically treated, prepared and divided into three groups according to dentine treatment (distilled water—DW, CHI 0.2% and EDC 0.5) and in three subgroups according to resin cement: RelyX ARC, Panavia F 2.0 or RelyX U200. Slices were obtained, with five slices of each third submitted to the analysis of the adaptation of the adhesive interface through scores and the perimeter with gaps in confocal laser scanning microscopy and one slice of each third later evaluated qualitatively in scanning electron microscopy. The results were analyzed using with Kruskal–Wallis and Spearman correlation tests. There was no difference in adaptation for the different resin cements (p = .438). EDC presented better adaptation when compared to the groups treated with DW and CHI (p < .001), while the CHI and DW presented similar adaptation values (p = .365). No difference was observed in the perimeter referring to the gap areas for the different resin cements (p = .510). EDC showed a lower percentage of perimeters with gaps when compared to CHI (p < .001), with the percentage of perimeter with gaps of teeth treated with CHI being lower than DW (p < .001). A positive correlation coefficient equal to 0.763 was obtained between the perimeter with gaps and the adaptation data of the adhesive interface (p < .001). EDC resulted in better adaptation of the adhesive interface and a lower percentage of perimeters with gaps compared to chitosan.
A DFT and kinetic study: Is it possible to prepare epoxides without catalysts using the in‐situ generated peroxy radicals or peroxides by one‐step method?
Authors: Wu Yufeng , Kang Jiajie , Gao Wei , Bi Mingshu , Yang Dongcheng , Ji Runlai , Meng Qingwei , Ma Cunfei 
Published: June 2023

AbstractDFT calculations and kinetic analysis have been employed to comprehensively explore the possibility to prepare epoxides by one‐step method using the in‐situ generated peroxy radicals or hydroperoxides as epoxidizing agents. Computational studies demonstrated that the selectivities for the reaction systems of O2/R2/R1, O2/CuH/R1, O2/CuH/styrene, O2/AcH/R1 were 68.2%, 69.6%, 100% and 93.3%, respectively. The in‐situ generated peroxide radicals, such as HOO˙, CuOO˙ and AcOO˙, could react with R1 or styrene by attacking the CC double bond to form a CO bond and subsequently undergoing a cleavage of OO bond to yield epoxides. Peroxide radicals could abstract a hydrogen atom from methyl group on R1, forming unwanted by‐products. It should be noted that the hydrogen atoms of HOO˙ is easy to be abstracted by CC double bond and simultaneously the oxygen atom is connected to the CH moiety to form an alkyl peroxy radical (Rad11), greatly limiting the selectivity. The comprehensive mechanistic studies provide a deep understanding on preparing epoxides by one‐step method.
T‐cell redirecting bispecific antibodies in multiple myeloma: Current landscape and future directions
Authors: O'Neill Chloe , van de Donk Niels W. C. J. 
Published: June 2023

AbstractT‐cell engaging bispecific antibodies (BsAbs) have substantial activity in heavily pretreated patients with multiple myeloma (MM). The overall response rate obtained with B‐cell maturation antigen (BCMA)‐targeting BsAbs is approximately 60%–70%, including a high proportion of patients achieving very good partial response or complete response. Comparable efficacy is seen with BsAbs targeting GPRC5D or FcRH5. Cytokine release syndrome is frequently observed with BsAb treatment, but mostly during the step‐up doses and the first full dose. Early intervention with IL‐6 receptor blocking antibodies (e.g., tocilizumab) prevents escalation to severe manifestations. Infections are also common during treatment and related to the extent of exposure to immune suppressive anti‐MM agents, as well as development of hypogammaglobulinemia due to elimination of normal plasma cells, and probably because of T‐cell exhaustion resulting from continuous BsAb‐mediated T‐cell activation. Adequate monitoring for infections and institution of infectious prophylaxis are essential. Patients treated with GPRC5D‐targteing BsAbs often develop skin and nail disorders and loss of taste, which is likely related to GPRC5D expression in cells that produce hard keratin. Currently ongoing studies are aiming at further improving these results by evaluating BsAbs in combination with other drugs, such as immunomodulatory agents and anti‐CD38 antibodies, as well as the application of BsAbs in earlier lines of therapy, including patients with newly diagnosed disease. We expect that the outcomes of patients with MM will further improve by the introduction of this novel type of T‐cell immunotherapy.
Use of nonstigmatizing language is associated with improved outcomes in hospitalized people who inject drugs
Authors: Carpenter Joseph E. , Catalanotti Jillian , Notis Melissa , Brokus Christopher , Moran Timothy P. , Akselrod Hana , Burkholder Greer , Eaton Ellen F. , Kuo Irene , Mai William , McGonigle Keanan , Steck Alaina , del Rio Carlos , Saag Michael , Kottilil Shyamasundaran , Masur Henry , Kattakuzhy Sarah , Rosenthal Elana S. 
Published: June 2023

AbstractBackgroundStigma surrounding opioid use disorder (OUD) is a barrier to treatment. The use of stigmatizing language may be evidence of negative views toward patients.ObjectiveWe aimed to identify associations between language and clinical outcomes in patients admitted for infectious complications of OUD.DesignsWe performed a retrospective medical record review.Settings and ParticipantsFour U.S. academic health systems. Participants were patients with OUD admitted for infectious complications of injection opioid use from January 1, 2018, to December 31, 2018, identified through international classification of diseases, 10th revision codes consistent with OUD and acute bacterial/fungal infection.Main Outcome and MeasuresDischarge summaries were reviewed for language, specifically: abuse, addiction, dependence, misuse, use disorder, intravenous drug use, and others. Binary outcomes including medication for OUD, planned discharge, naloxone provision, and an OUD treatment plan were evaluated using logistic regressions and admission duration was evaluated using Gamma regression.ResultsA total of 1285 records were reviewed and 328 met inclusion criteria. Of those, 191 (58%) were male, with a median age of 38 years. The most common term was “abuse” (219, 67%), whereas “use disorder” was recorded in 75 (23%) records. Having “use disorder” in the discharge summary was associated with increased odds of having a documented plan for ongoing OUD treatment (adjusted odds ratio [AOR]: 4.11, 95% confidence interval [CI]: 1.89–8.93) and having a documented plan for addiction‐specific follow‐up care (AOR: 2.31, 95% CI: 1.30–4.09).ConclusionsStigmatizing language was common in this study of patients hospitalized for infectious complications of OUD. Best‐practice language was uncommon, but when used was associated with increased odds of addiction treatment and specialty care referrals.
A mathematical model of SARS‐CoV‐2 immunity predicts paxlovid rebound
Authors: Ranard Benjamin L. , Chow Carson C. , Megjhani Murad , Asgari Shadnaz , Park Soojin , Vodovotz Yoram 
Published: June 2023

AbstractNirmatrelvir/ritonavir (Paxlovid), an oral antiviral medication targeting SARS‐CoV‐2, remains an important treatment for COVID‐19. Initial studies of nirmatrelvir/ritonavir were performed in SARS‐CoV‐2 unvaccinated patients without prior confirmed SARS‐CoV‐2 infection; however, most individuals have now either been vaccinated and/or have experienced SARS‐CoV‐2 infection. After nirmatrelvir/ritonavir became widely available, reports surfaced of “Paxlovid rebound,” a phenomenon in which symptoms (and SARS‐CoV‐2 test positivity) would initially resolve, but after finishing treatment, symptoms and test positivity would return. We used a previously described parsimonious mathematical model of immunity to SARS‐CoV‐2 infection to model the effect of nirmatrelvir/ritonavir treatment in unvaccinated and vaccinated patients. Model simulations show that viral rebound after treatment occurs only in vaccinated patients, while unvaccinated (SARS‐COV‐2 naïve) patients treated with nirmatrelvir/ritonavir do not experience any rebound in viral load. This work suggests that an approach combining parsimonious models of the immune system could be used to gain important insights in the context of emerging pathogens.
NFX1‐123: A potential therapeutic target in cervical cancer
Authors: Chintala Sreenivasulu , Dankoski Maura A. , Anbarasu Anand , Ramaiah Sudha , Miryala Sravan Kumar , Katzenellenbogen Rachel A. 
Published: June 2023

AbstractNFX1‐123 is a splice variant isoform of the NFX1 gene. It is highly expressed in cervical cancers caused by HPV, and NFX1‐123 is a protein partner with the HPV oncoprotein E6. Together, NFX1‐123 and E6 affect cellular growth, longevity, and differentiation. The expression status of NFX1‐123 in cancers beyond cervical and head and neck cancers, and its potential as therapeutic target, have not been investigated. TSVdb of TCGA was used to quantify NFX1‐123 expression in 24 cancers compared with normal tissues. The NFX1‐123 protein structure was predicted and then submitted to retrieve suitable drug molecules. The top four compounds, found to bind in silico to NFX1‐123, were tested experimentally to determine their effects on NFX1‐123‐related cellular growth, survival, and migration. 46% of cancers (11 of 24 had significant differences in NFX1‐123 expression, with nine having had greater NFX1‐123 expression, when compared with adjacent normal tissues. Bioinformatics and proteomic predictive analysis modeled the three‐dimensional structure of NFX1‐123, and drug libraries were screened for high‐binding affinity compounds using this modeled structure. Seventeen drugs with binding energies ranging from −1.3 to −10 Kcal/mol were identified. The top four compounds were used to treat HPV‐ and HPV+ cervical cancer cell lines, three of which (Ropitoin, R428 and Ketoconazole) reduced NFX1‐123 protein levels, inhibited cellular growth, survival, and migration, and enhanced the cytotoxicity of Cisplatin. These findings highlight cancers expressing high levels of NFX1‐123, and drugs that target it, may reduce cellular growth, survival, and migration, making NFX1‐123 a potential novel therapeutic target.
Diverse intratumoral heterogeneity and immune microenvironment of two HPV‐related cervical cancer types revealed by single‐cell RNA sequencing
Authors: Li Chunbo , Liu Danyang , Zhao Yu , Ding Yan , Hua Keqin 
Published: June 2023

AbstractCervical squamous cell carcinoma (SCC) and adenocarcinoma (AD) are the main histological types of human papillomavirus‐related cervical cancer. However, there are few reports on cell type‐specific molecular differences between SCC and AD. Here, we used unbiased droplet‐based single‐cell RNA sequencing to elucidate the cellular differences between SCC and AD in tumor heterogeneity, and tumor microenvironment (TME). A total of 61 723 cells from three SCC and three AD patients, were collected and divided into nine cell types. Epithelial cells exhibited high intra‐ and interpatient heterogeneity and functional diversity. Signaling pathways, such as epithelial‐to‐mesenchymal‐transition (EMT), hypoxia and inflammatory response were upregulated in SCC, while cell cycle‐related signaling pathways were highly enriched in AD. SCC was associated with high infiltration of cytotoxicity CD8 T, effector memory CD8 T, proliferative natural killer (NK), and CD160+ NK cells as well as tumor‐associated macrophages (TAMs) with high major histocompatibility complex‐II genes. AD exhibited a high proportion of naive CD8 T, naive CD4 T, Treg CD4, central memory CD8, and TAMs with immunomodulatory functions. Additionally, we also observed that the majority of cancer‐associated fibroblasts (CAFs) were from AD, and participated in inflammation regulation, while SCC‐derived CAFs exhibited similar functions to tumor cells, such as EMT and hypoxia. This study revealed the widespread reprogramming of multiple cell populations in SCC and AD, dissected the cellular heterogeneity and characteristics in TME, and proposed potential therapeutic strategies for CC, such as targeted therapy and immunotherapy.
Spatiotemporal variation and multivariate controls of short‐cycle drought–flood abrupt alteration: A case in the Qinling‐Daba Mountains of China
Authors: Song Xiaoyan , Lei Xiaoping , Ma Rui , Hou Jingzhi , Liu Wenbin 
Published: June 2023

AbstractA comprehensive understanding of the patterns of drought and flood alternation in adjacent months is essential for enabling climate adaptation and mitigation strategies. However, there is a paucity of knowledge regarding short‐cycle drought–flood abrupt alteration (s‐DFAA) and its responses to multiple environmental factors at various timescales. This is because of the inadequate formula construction of the existing short‐cycle drought–flood abrupt alteration index (SDFAI), such as the inability to introduce drought standards. To accurately capture the s‐DFAA's characteristics in the Qinling‐Daba Mountains (Qinba Mountains), we proposed a revised SDFAI (R‐SDFAI), which incorporates the standardized precipitation index (SPI) and allows for the customization of drought standards. Next, we used wavelet transform coherence and multiple wavelet coherence to investigate the timescale relationships between s‐DFAA events and their influence factors such as relative humidity, sunshine duration, temperature, evaporation, Arctic Oscillation (AO), Niño3.4 SSTA Index (Niño3.4), Total Sunspot Number Index (TSNI) and Pacific Decadal Oscillation Index (PDO). Our results showed that the R‐SDFAI outperformed traditional SDFAI in capturing s‐DFAA events and characterizing their severity. Furthermore, s‐DFAA events identified by R‐SDFAI at different levels (i.e., mild, moderate, severe, extreme and total) displayed insignificant downward trends. Spatially, there were more s‐DFAA events in the east than the west. Wavelet analysis indicated that meteorological factors and teleconnections significantly impact s‐DFAA events at large timescales, though their driving mechanisms differed substantially. Among meteorological factors, single relative humidity and its related combinations exhibited relatively high percent area of significant coherence (PASC, ranging from 17.47 to 29.46). Each teleconnection and its combinations are irreplaceable, with PASC values always increasing with the number of variables. The PASC ranges from 8.3 to 10.29 for one factor, 12.38 to 18.65 for two factors, 24.02 to 30.58 for three factors and 40.88 for four factors, respectively.
Influence of boreal summer intraseasonal oscillation on summer precipitation in North China
Authors: Hao Lisheng , Li Tim , Zhang Lujun , Ma Ning 
Published: June 2023

AbstractTo understand the impact of the boreal summer intraseasonal oscillation (BSISO) propagation on summer precipitation in North China, this study analysed the quantitative relationship between the BSISO and summer precipitation in North China and its influence mechanism using summer precipitation data from 1981 to 2020, the NCEP/NCAR reanalysis data and the NOAA outgoing longwave radiation (OLR). The results show that there is a strong correlation between summer precipitation in North China and the BSISO. Over the past 40 years, the variance in summer precipitation anomalies in North China caused by BSISO1 accounted for 16.8%, whereas the impact of BSISO2 accounted for 13.1%. Their combined impact accounted for 30% of the variance in summer precipitation in North China. The BSISO influences summer precipitation process in North China primarily through upper tropospheric circulation and lower‐level water vapour transport. At 500 and 200 hPa, the BSISO excited some disturbance centres of a deep barotropic structure propagating from west to east along the upper‐tropospheric westerly jet. North China is controlled by the blocking circulation pattern of “high in the east and low in the west” while there is a negative (positive) anomaly near Lake Baikal and a positive (negative) anomaly near the Korean Peninsula. At 850 hPa, the BSISO stimulates an anticyclonic circulation anomaly on the north side of its convective area, and the southerly winds on the west side of the anticyclone enhance water vapour transport to North China. This high‐ and low‐level circulation configuration is favourable for precipitation processes in North China. The results suggest that the influence of both low‐frequency signals in the middle and high latitudes and the BSISO signal in the tropics should be considered to make extended‐range forecasts of precipitation processes in North China.