bluuebunny/crossref_metadata_2025_split huggingface dataset:
Crb3 and NF2: A dynamic duo that controls assembly of the apical junctions and barrier function via Rho/ROCK signaling
Authors: Fan S. , Varadarajan S. , Garcia-Hernandez V. , Margolis B. , Parkos C.A. , Nusrat A. 
Published: January 2025

AbstractThe gastrointestinal epithelium serves as a critical barrier separating intestinal lumen contents from the underlying tissue environment. Structure and function of the apical junctional complex (AJC), comprising tight and adherens junctions, are essential for establishing and maintaining a polarized and functional epithelial barrier. In this study, we investigated mechanisms by which an apical polarity protein Crumbs homolog 3 (CRB3) regulates AJC assembly and barrier function in primary murine intestinal epithelial cells. Using primary colonic epithelial cells (colonoids) derived from inducible and conditional Crb3 knockdown (Crb3ERΔIEC) and control mice (Crb3fl/fl), we demonstrate that Crb3 loss leads to compromised epithelial barrier function that was associated with hypercontractile perijunctional actomyosin and defective assembly of the AJC. We identified CRB3 associates with the Band 4.1 family of cytoskeletal linker proteins, Merlin (NF2) via FERM (band4.1/ezrin/radixin/moesin) binding domain (FBD) of CRB3. Interestingly, NF2 knockdown in cultured intestinal epithelial cells phenocopied the effect of CRB3 deletion, supporting a coordinated role in AJC formation and barrier assembly. Moreover, increased active Rho was detected in assembling junctions of Crb3-null cells and inhibition of ROCKII and myosin II alleviated the hypercontractile phenotype, highlighting involvement of Rho/ROCK signaling. Additionally, increased vinculin localization at the AJC seen in Crb3-null epithelial cells indicates elevated tension at junctions. Our findings underscore the important role of Crb3 and NF2 in regulating contractility of the perijunctional actomyosin ring, mechanical tension at the AJC and barrier function via Rho/ROCK signaling during junctional assembly in intestinal epithelial cells.
Immunization with a novel RNA replicon vaccine confers long-lasting protection against H5N1 avian influenza virus in 24 bird species
Authors: Stettler Marion , Hoby Stefan , Wenker Christian , Wyss Fabia , Heiderich Elisabeth , Butticaz Lisa , Ruggli Nicolas , Darpel Karin , Zimmer Gert 
Published: January 2025

AbstractHighly pathogenic avian influenza viruses (HPAIV) of subtype H5N1 (clade 2.3.4.4b) have spread worldwide and caused the death of hundreds of millions of wild birds and domestic poultry. Moreover, spill over of H5N1 HPAIV from infected birds to more than 50 different mammalian species including humans has been recorded. While, licensed vaccines for protection of avian or mammalian species are not yet available, a few candidate vaccines are being trialled. Here, we report on the experimental vaccination of chickens and captive wild birds using a propagation-defective vesicular stomatitis virus (VSV), in which the essential envelope glycoprotein (G) protein gene was replaced by a modified hemagglutinin gene derived from a clade 2.3.4.4b H5N1 isolated in 2022 in the animal park of Bern, Switzerland. VSVΔG(H5mb) was produced on helper cells providing the VSV G proteinin trans. Specific pathogen-free (SPF) chickens that were immunized twice via the intramuscular route with adjuvant-free VSVΔG(H5mb) replicon particles induced high levels of virus-neutralizing serum antibodies and were fully protected against lethal infection by H5N1 HPAIV (clade 2.3.4.4b). Notably, immunized animals did not shed challenge virus from the respiratory or gastrointestinal tract, suggesting that herd immunity can be achieved. The same vaccine was used to immunize a total of 317 captive wild birds at Bern Animal Park and Zoo Basel, representing 24 different species. No vaccine-associated side effects were observed. Birds without previous contact to H5Nx viruses produced high to very high H5-specific neutralizing antibody titers following the second immunization, while birds showing H5-specific antibodies prior to vaccination, already developed high neutralising antibody titers after a single immunization. One year after vaccination, most animals still showed significant neutralizing antibody titers, indicating that VSVΔG(H5mb) is able to induce a long-lasting protective immune response. Our results indicate that VSVΔG(H5mb) is an extraordinary safe and highly efficacious vaccine to stop H5N1 replication in various avian species.
PI31 is a positive regulator of 20S immunoproteasome assembly
Authors: Wang Jason , Kjellgren Abbey , DeMartino George N. 
Published: January 2025

ABSTRACTPI31 (Proteasome Inhibitor of31,000 Da) is a 20S proteasome-binding protein originally identified as an inhibitor ofin vitro20S proteasome activity. Although recent studies have provided a detailed structural basis for this activity, the physiologic significance of PI31-mediated proteasome inhibition remains uncertain and alternative cellular roles for PI31 have been described. Here we report a role for PI31 as a positive regulator for the assembly of the 20S immuno-proteasome (20Si), a compositionally and functionally distinct isoform of the proteasome that is poorly inhibited by PI31. Genetic ablation of PI31 in mammalian cells had no effect on the cellular content or activity of constitutively expressed proteasomes but reduced the cellular content and activity of interferon-γ-induced immuno-proteasomes. This selective effect is a consequence of defective 20Si assembly, as indicated by the accumulation of 20Si assembly intermediates. Our results highlight a distinction in the assembly pathways of constitutive and immuno-proteasomes and indicate that PI31 plays a chaperone-like role for the selective assembly of 20S immunoproteasomes.
Structural mechanism for recognition of E2F1 by the ubiquitin ligase adaptor Cyclin F
Authors: Ngoi Peter , Wang Xianxi , Putta Sivasankar , Da Luz Ricardo F. , Serrão Vitor Hugo B. , Emanuele Michael J. , Rubin Seth M. 
Published: January 2025

AbstractCyclin F, a non-canonical member of the cyclin protein family, plays a critical role in regulating the precise transitions of cell-cycle events. Unlike canonical cyclins, which bind and activate cyclin-dependent kinases (CDKs), Cyclin F functions as a substrate receptor protein within the Skp1-Cullin-F box (SCF) E3 ubiquitin ligase complex, enabling the ubiquitylation of target proteins. The structural features that distinguish Cyclin F as a ligase adaptor and the mechanisms underlying its selective substrate recruitment over Cyclin A, which functions in complex with CDK2 at a similar time in the cell cycle, remain largely unexplored. We utilized single-particle cryo-electron microscopy to elucidate the structure of a Cyclin F-Skp1 complex bound to an E2F1 peptide. The structure and biochemical analysis reveal important differences in the substrate-binding site of Cyclin F compared to Cyclin A. Our findings expand on the canonical cyclin-binding motif (Cy or RxL) and highlight the importance of electrostatics at the E2F1 binding interface, which varies for Cyclin F and Cyclin A. Our results advance our understanding of E2F1 regulation and may inform the development of inhibitors targeting Cyclin F.
Growth in Low Carbon Conditions Reveals Amino-Acid-Coupled Iron Uptake
Authors: Lara-Gutiérrez Juanita , Nguyen Jen , McIlvin Matthew R. , Sugiyama Ichiko , Landry Zachary , Alcolombri Uria , Keegstra Johannes M. , Pontrelli Sammy , Jiménez-Martínez Joaquín , Sauer Uwe , Hwa Terence , Saito Mak A. , Stocker Roman 
Published: January 2025

AbstractBacteria in nature encounter substrates at widely varying concentrations, yet studies of bacterial physiology have focused more on nutrient type than concentration, partly due to challenges in maintaining low concentrations. We developed a Millifluidic Continuous Culture Device (MCCD) to culture bacteria under precisely controlled nutrient conditions, including very low concentrations, in a manner suitable for proteomic analysis. Using the MCCD, we culturedEscherichia coliwith a mixture of amino acids as the sole carbon source at three concentrations supporting growth rates spanning a fivefold range. Surprisingly, at the lowest concentration, cells exhibited proteomic signatures of iron shortage despite equal iron levels across conditions. We observed the uptake of labeled iron-histidine and iron-cysteine complexes, indicating that amino acids facilitated iron acquisition and that amino-acid-bound iron is bioavailable toE. coli. These findings reveal a previously unknown mechanism of bacterial iron acquisition that emerged under the flow imposed by the MCCD, which likely diluted the siderophore pool and reduced their efficacy. This work highlights the importance of studying bacterial physiology under low nutrient concentrations and demonstrates how physical conditions, such as flow, shape microbial nutrient acquisition strategies.
Parenclitic network mapping predicts survival in critically ill patients with sepsis
Authors: Ito Emily , Oyelade Tope , Wikner Matthew , Liu Jinyuan , Lilaonitkul Watjana , Mani Ali R 
Published: January 2025

AbstractSepsis is a complex disease involving multiple organ systems. A network physiology approach to sepsis may reveal collective system behaviours and intrinsic organ interactions. However, mapping functional connectivity for individual patients has been challenging due to the lack of analytical methods for evaluating physiological networks using routine clinical and laboratory data. This study explored the use of parenclitic network mapping to assess organ connectivity and predict sepsis outcomes based on routine laboratory data. Data from 162 sepsis patients meeting Sepsis-3 criteria were retrospectively analysed from the MIMIC-III database, focusing on 48-hour deterioration and 30-day survival. Fifteen physiological variables representing organ systems were used to construct organ network connectivity through correlation analysis. Correlation analysis identified 7 organ interactions linked to 30-day survival and 9 related to 48-hour deterioration. Parenclitic network analysis was used to measure deviations in individual patients’ correlations between organ systems from the reference physiological interactions observed in survivors. Parenclitic deviations in the pH-bicarbonate axis (hazard ratio=2.081, p<0.001) and pH-lactate axis (hazard ratio=2.773, p=0.024) significantly predicted 30-day mortality, independent of the Sequential Organ Failure Assessment (SOFA) score and ventilation status. However, parenclitic deviations did not predict 48-hour deterioration. This study highlights the potential of parenclitic network mapping to provide insights into sepsis pathophysiology and differences in organ system connectivity between survivors and non-survivors independent of sepsis severity and mechanical ventilation status. Integrating physiological network mapping with current prognostic tools may enhance personalized sepsis management and outcome prediction in clinical settings.New and NoteworthyThis study employs an integrative network analysis approach to investigate sepsis, aiming to leverage routine laboratory data for prognostication and to enhance understanding of the pathophysiology of sepsis. Using parenclitic network mapping, we assessed individual organ connectivity patterns that distinguish between survivors and non-survivors. Our findings demonstrate that physiological network mapping in sepsis offers prognostic insights independent of disease severity and mechanical ventilation status in critically ill patients with sepsis.
How do vocational students evaluate vocational education internships – an evaluation scale development in China
Authors: Yan Cailing 
Published: January 2025

PurposeThis research aims to construct an Evaluation Scale for Vocational Education Internships (ESVEI) tailored to assess the vocational educational internship experiences from the perspectives of vocational students. It also seeks to investigate the influence of certain factors – such as gender, family background, educational attainment, chosen major and the duration of the internship – on the assessment of these internships.Design/methodology/approachIn the development of ESVEI, a combination of methods was employed, including literature analysis, item refinement, exploratory factor analysis, confirmatory factor analysis, multiple group analysis and reliability analysis. A total of 1,114 vocational students who had finished their internships completed questionnaires formulated based on ESVEI.FindingsA two-dimensional evaluation tool called the ESVEI, consisting of the “Internship Content Evaluation” (ICE) and “Internship Supervisor Evaluation” (ISE) with seven items, has been created. Based on this, the corresponding questionnaires’ results indicate that female respondents, those from urban areas and those in the medical and nursing fields generally rated their internships more favorably compared to their male, rural and engineering and manufacturing counterparts. Additionally, when the internships last less than six months, the longer the internship, the more favorable evaluations.Research limitations/implicationsBecause of the limited number of respondents, the research results may lack generalizability. Therefore, researchers are encouraged to test the proposed evaluation scale further.Practical implicationsThe scale developed in this study serves as a tangible instrument for assessing the vocational education internship experiences of vocational students. Furthermore, this analysis can provide valuable insights for identifying areas that require further growth and improvement based on the dimensions of internship content and the trainers involved.Originality/valueThis is one of the first studies to develop an evaluation scale for vocational education internships in China, which not only clarifies the evaluation dimensions of internships but also forms a tool for future research.
Green exploitation and exploration innovation as responses to environmental performance shortfalls: integration of performance feedback, threat-rigidity and ambidexterity perspectives
Authors: Lee Hanna 
Published: January 2025

Purpose
Building on performance feedback and threat-rigidity theories, this study aims to argue that environmental performance shortfalls are powerful motivators for shaping green innovation strategies. To examine our argument, this study extends the theoretical logic to organizational ambidexterity and examines green exploitation and exploration innovation and relative green ambidexterity as organizational responses to environmental performance shortfalls. This study addresses how environmental performance shortfalls affect the implementation of green exploitation and exploration innovation and relative green ambidexterity and how this chosen green innovation strategy affects corporate environmental performance.

Design/methodology/approach
The authors test their theory using 145 sample firms’ environmental performance data spanning 2015 to 2021 and conduct a content analysis of their 697 sustainability reports spanning 2017 to 2021.

Findings
This paper finds that environmental performance shortfalls positively affect green exploitation innovation and relative green exploitation innovation and negatively affect green exploration innovation and relative green exploration innovation. It also finds that green exploration innovation and relative green exploration innovation positively affect environmental performance but that green exploitation innovation and relative green exploitation innovation negatively affect environmental performance.

Originality/value
The findings support the premise that environmental performance feedback guides the direction of strategic choices and actions related to green innovation and confirm the decisive role of green exploration innovation in improving environmental performance. This study augments performance feedback, threat-rigidity and organizational ambidexterity theories in the environmental management context.
Do we need harsh punishment? The effect of coercive power in collaborative consumption services
Authors: Bhalla Saubhagya , Kansal Purva 
Published: January 2025

Purpose
The study aims to investigate factors impacting consumers’ intention to adopt collaborative consumption (CC, hereafter) services. It extends the existing knowledge of CC services by testing and validating the moderating role of coercive power and the mediating role of attitude.

Design/methodology/approach
Applying random sampling, the data was collected through the mall intercept method across four cities in India and was analyzed using partial least square-structural equation modeling and artificial neural network to test the proposed hypotheses. A follow-up qualitative study consisting of 15 in-depth structured interviews helped validate the results of the quantitative research.

Findings
Results of the conducted survey revealed that a sense of community, reason-based trust and enjoyment positively impact consumers’ attitudes toward CC services. Attitude positively impacts intention and shows a partial mediating influence on the relationship between motivations and intentions. Furthermore, the results revealed the moderation of the perceived coercive power of the service provider. The results of the follow-up qualitative study validated the results of the quantitative research.

Practical implications
Managers of CC firms must understand the relative importance of intrinsic and extrinsic motivations in formulating the attitudes of consumers and intentions toward CC services. CC managers should acknowledge the influence of the consumer’s perceived coercive power of service providers and consumer’s reason-based trust.

Originality/value
To the best of the authors knowledge, the current research is the first of its kind. It has justifiably and logically applied self-determination theory and a slippery slope framework in a single context. By testing the moderating impact of coercive power, the research extends the existing literature on CC and the applicability of coercive power in CC. The present study extends the knowledge regarding the consumer’s perception of reason-based trust and the perceived coercive power of service providers in CC.
The extent and determinants of SDG disclosures in Vietnamese listed firms
Authors: Nguyen Huu Cuong , Duong Hien Khanh 
Published: January 2025

Purpose
The purpose of this study is to assess the extent of Sustainable Development Goals (SDGs) disclosures among Vietnamese listed firms and identify key influencing factors.

Design/methodology/approach
The authors analyse SDG-related disclosures of the top 100 listed firms by market capitalisation on the Hanoi and Ho Chi Minh stock exchanges as of December 31, 2023, using an established reporting methodology. Data were sourced from annual, corporate governance, ESG, financial and sustainability reports. A regression model was used to examine factors influencing SDG disclosure.

Findings
SDG disclosure among Vietnamese firms is relatively low. Corporate governance, firm size, government ownership, industry and Global Reporting Initiative (GRI) usage positively influence disclosure levels, while auditing firm type and firm age show a negative association. Financial firms tend to use sustainable development reports and GRI indicators more frequently.

Practical implications
Practically, strengthening governance frameworks and promoting GRI adoption can improve the quality and extent of sustainability reporting among Vietnamese firms. Socially, enhanced SDG disclosure supports improved corporate practices that align with the United Nations SDGs, fostering a more sustainable and transparent economy in Vietnam.

Originality/value
To the best of the authors’ knowledge, this is the first study examining SDG disclosure and influencing factors in Vietnamese listed (2021–2023), using the GRI (2016) standard. This study contributes to transparency in Vietnam’s financial markets and sustainability practices, offering insights for preparers and policymakers.
Unpacking role of environmental awareness in the triad relationship of religious commitment, environmental values and intentions in Indian textile industry
Authors: Singh Sandeep , Arora Muskaan , Kaur Rajinder , Rani Amita , Kathuria Payal 
Published: January 2025

Purpose
This study aims to examine the relationship between religious commitment (RC), environmental values (EVs), behaviour intentions and awareness. In this study, EV is studied as a mediator between the relationship of RC and environmental behavioural intentions. Finally, this study examines the conditional effect of environmental awareness on this mediation.

Design/methodology/approach
This study was conducted in the textile industry in Punjab, India. The sample for this study was 259 managers of different textile firms. The data was collected on standardised scales. Partial least squares structural equation modelling was used to examine the direct and indirect effects in this study.

Findings
It was found that the indirect effect between RC and environmental behaviour intention was significant through EV. This study confirmed that the mediation between the variables was moderated by environmental awareness.

Practical implications
Based on the tested model, textile organisations can improve their corporate social responsibility practices. This study highlights the importance of encouraging environmental awareness among textile industry employees, especially those with religious convictions, to reduce environmental impacts. It suggests ecological training, education initiatives and implementing eco-friendly policies.

Originality/value
This study provides a model based on social and psychological factors. The findings of this study can be applied in the textile industry to augment environmentally friendly behaviour among managers. The perspective of moderated meditation among the relationships of variables carries a unique value in this study. Furthermore, this study combines social factors like religion with environmental psychology within business settings. In this way, study carries a unique value.