bluuebunny/crossref_metadata_2025_split huggingface dataset:
Short-term effect of plant-based Nordic diet versus carbohydrate-restricted diet on glucose levels in gestational diabetes – the eMOM pilot study
Authors: Markussen Lisa Torsdatter , Kivelä Jemina , Lindström Jaana , Ashrafi Reza A. , Heinonen Seppo , Koivusalo Saila , Meinilä Jelena 
Published: March 2023

Abstract
Background

The optimal nutritional treatment for gestational diabetes (GDM) is still a matter of debate. With increasing rates of GDM and potential negative consequences for the health of mother and child, the best treatment should be established. The Nordic diet with emphasis on plant-based protein shows promising health outcomes in other populations but has yet to be investigated in GDM population. The aim of the eMOM pilot study was to compare the short-term effects of healthy Nordic diet (HND) and the currently recommended moderate restriction of carbohydrates diet (MCRD) on glucose and lipid metabolism in women with GDM.

Methods

This was a 14-day randomized crossover diet intervention. In total, 42 pregnant women diagnosed with GDM (< 29 + 0 gestational week) were randomized. The run-in to the crossover was three days of the participants’ regular diet, followed by the first allocated three-day intervention diet. This was followed by the second three-day intervention diet. The intervention diets were separated by a three-day wash-out period consisting of the participants’ regular diet. All participants wore a continuous glucose monitor (CGM, Freestyle Libre®, Abbott, USA) for 14 days, and gave blood samples four times during the crossover. The primary outcome was time spent in glucose target range (TIR, < 7.8 mmol/L). TIR, 3-day mean tissue glucose as well as changes in fasting glucose, homeostatic model of insulin resistance (HOMA-IR) and blood lipids were analyzed with paired samples statistical analyses.

Results

36 women with complete 14 days CGM data were analyzed. Both diet interventions produced a high degree of TIR (98% SD 1.8), without a difference between the diets (p = 0.727). The 3-day mean glucose was significantly lower in HND than in MCRD (p = 0.049). Fasting insulin (p = 0.034), insulin resistance (p = 0.03), total and LDL cholesterol (p = 0.023 and 0.008) reduced more in the MCRD diet than the HND. NS differences in any other measure of CGM or blood tests.

Conclusions

HND and MCRD did not differ in terms of their short-term effect on TIR. A larger study with sufficient power is needed to confirm the differences in short-term mean glucose, insulin resistance and lipid metabolism.
Trial registration: Registered in clinicaltrials.gov (21/09/2018, NCT03681054).
Efficient photo-adsorptive eradication of endocrine disrupting pesticides by chitosan co- decorated metal oxide bio-nanocomposite
Authors: Yadav Jyoti , Rani Manviri , Zhang Tian C , Shanker Uma 
Published: March 2023

Abstract
Extensive consumption, toxicity, and bioaccumulation of malathion (MLT) and lindane (γ-HCH) pesticides collectively attract the world’s attention. Herein, the nanocomposite of chitosan wrapped NiO@ZnO was synthesized by a green methodology using Azadirachta indica leaves extract. Structural and morphological analysis of chitosan-NiO@ZnO showed hollow sphere-flake shaped image adsorbed on a solid chitosan surface with a large surface area of 73 m2g-1. A decrease in values of lattice strain, dislocation density and crystallite size described the imperfection in crystal geometry and new peaks in FT-IR spectra at 698 cm-1 and 448 cm-1 of Ni-N and Zn-N, respectively confirms the coupling. The chitosan- NiO@ZnO nanocomposite with a reduced band gap due to the generation of new energy levels in the vicinity of of conduction and valence band. Chitosan-NiO@ZnO and individuals nanoparticles (NiO and ZnO) were well-characterized and utilized for degradation MLT and γ- HCH under direct sunlight and dark conditions. The highest degradation of pesticides (above 94%) resulted with 2 mg L-1 and 10 mg L-1 of MLT (π_π) and γ- HCH, respectively with a 20 mg catalyst dose, and pH of ~7 under daylight exposure (5 h). Chitosan-NiO@ZnO substantially suppressed the half-life of the targeted pesticides (MLT: 0.48 h; HCH 0.51 h) and demonstrated the first- order kinetics with a high adsorption capacity, Xm (MLT: 14.5 mg g-1 and γ- HCH 20.7 mg g-1), which also confirmed the strong binding with the pesticides, followed by their conversion into safer and smaller metabolites. The charge separation mechanism was elucidated by UV reflectance and photoluminescence data. Hydroxyl radicals were most frequently responsible for the degradation of pesticides as confirmed by scavenger analysis. The synthesized green-nano photocatalyst showed high reusability (up to 10th cycles), sensitivity, and stability within the degradation process, presumably making it suitable for industrial applications.
Experimental study on eye movement characteristics of fatigue of selected college students
Authors: Zhao Qian , Nie Baisheng , Bian Tingting , Ma Xinyu , Lin Shuangshuang , Wang Kedi , Meng Junqing 
Published: March 2023

Abstract
Fatigue is a complex physiological phenomenon that reflects worsening physical function and can lead to poor work performance, safety and health conditions. The research targets to obtain the degree of eye fatigue of college students in fixed sitting posture. An eye movement detection method was proposed in this paper. Using the wireless transmission eye tracker and its supporting software from the American Laboratory of Applied Sciences (ASL), three basic types of eye movements were examined, including blink, gaze, and saccade. The results show that with the increase of fatigue, participants’ blink time increased first and then decreased. During fatigue, the pupil diameter increased by about 91% and the percentage of fixation time decreased by about 31.31%. Compared with waking state, the average fixation time increased by about 9%, saccade distance decreased by about 40%. The research results can provide reference for fatigue monitoring and accident prevention.
The impact of green credits on high-quality energy development: Evidence from China
Authors: Cai Shuya , Zheng Ziyan , Wang Yi , Yu Maojun 
Published: March 2023

Abstract
The implementation of green credits has become an important engine for China's high-quality energy development (HQED). On the basis of constructing an index of HQED and the panel data of thirty provinces in China from 2008 to 2019, this study empirically investigated the effects of green credits on HQED and the action mechanisms behind it in a multi-dimensional manner using a panel fixed-effects model, mediating-effects model, and spatial Durbin model. The results indicated that green credits had significantly contributed to China's HQED, and that conclusion still held true after a series of robustness tests were conducted. It was found that industrial structures and human capital were important channels through which green credits influenced China’s HQED. Moreover, the spatial spillover effects of green credits on HQED were also confirmed. Finally, in terms of temporal heterogeneity, the positive effects of green credits on HQED were found to have increased significantly after 2012. Also, in terms of regional heterogeneity, this study observed that the positive influence of green credits on HQED was more significantly in central and western China than in eastern China, and in southern China than in northern China. The results obtained in this research investigation will potentially provide some important insights for energy planners and policymakers to further the understanding of the drivers of HQED, and the corresponding transmission mechanisms and regional differences.
Identification and pathogenic (toxicogenic) potential of Staphylococcus haemolyticus and Bacillus paranthracis consortia isolated from bovine mastitis in Russia
Authors: Sokolov Sergei , Brovko Fedor , Solonin Alexander , Nikanova Daria , Fursova Ksenia , Artyemieva Olga , Kolodina Evgenia , Sorokin Anatoly , Shchannikova Margarita , Dzhelyadin Timur , Ermakov Artem , Boziev Khanafy , Zinovieva Natalia 
Published: March 2023

Abstract
Bovine mastitis is one of the most economically important diseases in cattle. Infectious mastitis has a viral or bacterial origin. The most common bacterial agents are Staphylococcus, Streptococcus, coliforms, and Bacillus species. These are opportunistic microorganisms that can switch to a pathogenic form, using various virulence factors with follow various pathologies, both animal and human. Three stable microbial consortia, each composed of Bacillus paranthracis and Staphylococcus haemolyticus strains, were isolated from milk of cows diagnosed with mastitis in three geographically remote regions of Russia. The composition of these consortia remained stable following multiple passages on culture media. Apparently, this stability is due to the structure of the microbial biofilms formed by the communities. The virulence of the consortia depended on the B. paranthracis strains. It seems plausible that the ability of the consortia to cause mastitis in cattle was affected by mutations of the cytK gene of B. paranthracis.
Evaluation of toxicity and biocompatibility of a novel Mg-Nd-Gd-Sr alloy in the osteoblastic cell
Authors: Xie Yadong , yang qinglin , Liu Xiaorong , xie ben , Zhang Xiaobo , wang yongping 
Published: March 2023

Abstract
Background
We investigated the toxicity and biocompatibility of a novel Mg-3Nd-1Gd-0.3Sr-0.2Zn-0.4Zr (abbreviated to Mg-Nd-Gd-Sr) alloy in the osteoblastic cell line MC3T3-E1 as osteoblasts play an important role in bone repair and remodeling. Methods: We used cytotoxicity tests and evaluation of cell damage and apoptosis to investigate the effects of the Mg-Nd-Gd-Sr alloy on osteoblastic cells. Cell bioactivity, cell adhesion, cell proliferation, mineralization, ALP activity, and expression of BMP-2 and OPG by osteoblastic cells were also used to investigate the biocompatibility of Mg-Nd-Gd-Sr alloy.
Results
The results showed that the Mg-Nd-Gd-Sr alloy had no obvious cytotoxicity, and did not induce apoptosis or cause damage to MC3T3-E1 cells. Compared with the control group, the number of adherent cells within 12 hours was increased significantly in each experimental group (P < 0.05); the OD value of MC3T3-E1 cells was increased significantly in each experimental group on days 1 and 3 of culture (P < 0.05); the number of mineralized nodules formed in each experimental group was significantly increased (P < 0.05), and ALP activity was significantly increased in each experimental group (P < 0.05). RT-PCR results showed that the mRNA expression of BMP-2 and OPG was significantly higher in each experimental group compared with the control group (P < 0.05). Western blotting showed that the Mg-Nd-Gd-Sr alloy extract significantly increased the protein expression of BMP-2 and OPG compared with the control group (P < 0.05).
Conclusions
Our data indicated that the novel Mg-Nd-Gd-Sr-Zn-Zr alloy had no obvious cytotoxic effects, and did not cause apoptosis or damage to MC3T3-E1 cells; meanwhile it promoted cell adhesion, cell proliferation, mineralization, and ALP activity of osteoblasts. During this process, there was an increase in the expressions of BMP-2 and OPG mRNAs and proteins.
Comparison of 7-Year, Real World Clinical Outcomes Between Drug-Coated Balloon Angioplasty Versus Drug-Eluting Stent Implantation in Patients with Drug-Eluting Stent In-stent Restenosis
Authors: Kim Minsu , Jang Albert Youngwoo , Lee Joonpyo , Seo Jeongduk , Shin Yong Hoon , Oh Pyung Chun , Suh Soon Yong , Lee Kyounghoon , Kang Woong Chol , Han Seung Hwan 
Published: March 2023

Abstract
There are no data comparing the real-world, long-term clinical outcomes of drug-eluting balloon (DEB) angioplasty and drug-eluting stent (DES) implantation in DES-in-stent restenosis (ISR) lesions. We aimed to compare the real-world, long-term data between DEB and DES in DES-ISR lesions. We analyzed consecutive DES-ISR lesions (225 lesions from 205 patients; male: 66.3%; mean age: 62.4 years) treated with DEB or DES. The primary endpoint was target lesion revascularization (TLR) and the primary safety endpoint was the lesion-oriented composite outcome (LOCO). LOCO is composed of cardiac death, myocardial infarction, or target lesion thrombosis during follow-up. During the 7-year follow-up period, TLR did not differ significantly between DEB (n=108) and DES (n=117) (hazard ratio, 1.07; 95% CI 0.59–1.93, P=0.83). LOCO was significantly lower in the DEB group than in the DES group (hazard ratio, 0.40; 95% CI 0.16–0.98, P=0.04), which was mainly driven by less myocardial infarction (HR 0.24, 95% CI, 0.06–0.94, P=0.04) and absence of target lesion thrombosis in the DEB group (vs. DES group 6%, P=0.02). Additionally, cardiac death was similar between DEB and DES groups (HR 0.56, 95% CI 0.18–1.75, P=0.32).DEB angioplasty showed favorable safety with similar efficacy to that of DES implantation in DES-ISR lesionsduring the long-term follow-up period.
Green synthesis of copper nanoparticles using Artemisia annua aqueous extract and its characterization, antioxidant and burn wound healing activities
Authors: Karimi Behnaz , Mardani Masoumeh , Kaboutari Jahangir , Javdani Moosa , Albadi Jalal , Shirian Sadegh 
Published: March 2023

Abstract
the current study reports a green method for synthesizing copper nanoparticles (CuNPs) using aqueous extract of Artemisia annua in a simple, and eco-friendly manner. The green synthesized CuNPs were characterized using UV–Vis, FT-IR spectroscopy, X-ray diffraction (XRD), scanning electron microscopy (SEM) and Energy Dispersive X-ray analysis (EDX). The UV-Vis spectroscopy showed maximum absorption at 250 nm, and the average diameter of the synthesized NPs was found to be 35 nm. FTIR showed the presence of functional groups in bioactive constituents responsible for reducing Cu+ ions. The antioxidant activity of the synthesized NPs was evaluated by DPPH assay, and at 100 µg/ml green synthesized CuNPs, the highest antioxidant activity was obtained, which was 67 % and an IC50 value of 73.48 µg/ml. In the in vivo experiment, after inducing 2nd degree burn wounds, the rats were randomly divided into five group including the untreated control, treatment with eucerin basal ointment, treatment with 0.2 % CuNP ointment, treatment with 0.2 % CuNP-loaded ointment, and treatment with 5 % Artemisia annua extract twice a day for 21 days. Application of biosynthesized CuNPs ointment significantly improved the healing percentage and hydroxyproline content compared to the control. Additionally, histopathology studies showed significant progress in re-epithelization, collagen fiber deposition, and angiogenesis in the biosynthesized CuNPs in comparison to the control. The greenly synthesized CuNPs had enhanced antioxidant, and wound healing properties and, therefore, could be considered as a promising candidate in the biomedical field.
Ground strains induced by the 2022 Hunga-Tonga volcanic eruption, observed by a 1500-m laser strainmeter in Kamioka, Japan
Authors: Takamori Akiteru , Araya Akito , Miyo Kouseki , Washimi Tatsuki , Yokozawa Takaaki , Hayakawa Hideaki , Ohashi Masatake 
Published: March 2023

Abstract
In this study, we detected the horizontal ground strains, induced by the atmospheric Lamb wave emitted from the 2022 eruption of the Tong–Hunga Ha’apai undersea volcano, at an underground observatory in Kamioka, Japan. The observed strains were in the range of 10-11 to 10-10 and precisely measured using a 1500-m laser strainmeter with a high resolution of the order of 10-12. The strainmeter was constructed in a tunnel of KAGRA gravitational-wave telescope. Our observations showed that strain and atmospheric pressure were clearly correlated, resulting in a regression coefficient of -(2.3 –3.7) × 10-10 strain/hPa. This finding was compared with the responses under regular pressure conditions and the estimations by the local deformation model and the traveling wave model. The observed coefficients for the Lamb wave had smaller magnitudes than those under regular conditions and has a value between that of the two theoretical models. These results reflect the intermediate scale of pressure distribution of the Lamb wave between the assumptions of the simple models. The strain variations were also found to have started earlier than the corresponding pressure changes at the observation site with a characteristic time shifts ranging in 25–155 seconds. This finding indicates that the remote pressure loading caused the ground strains, and such interpretation was enabled by the specific pressure distribution of the Lamb wave.
Interactions between self-reported pain intensity and affects in daily life: a pilot study comparing individuals with chronic pain with primary and secondary pain disorders
Authors: Ledermann K. , Piccolo Mayron , Recabarren R. , Blanc J. , Martin-Soelch Ch. , Sprott H. 
Published: March 2023

Abstract
Background and objectives:
Chronic pain is frequently associated with depression and anxiety disorders. A relationship between affects and pain intensity in everyday life has been previously documented using Ecological momentary assessment technique (EMA) with a particular focus on the exacerbating effect of stress and depressive symptomatology on pain intensity in daily life of specific samples of chronic pain patients. With the introduction of the new category of chronic primary pain in the ICD-11, which is strongly characterized by emotional distress, it is interesting to compare primary and secondary chronic pain patients with regard to the relationships between pain intensity and affects in everyday life.
Methods:
Here, 16 individuals with chronic pain (primary pain, N=10; secondary pain, N=6) completed a pain diary during 2 consecutive weeks, 3 times a day, within fixed time periods (morning, midday, evening). Self-report questionnaires were used to measure depressive symptomatology and anxiety. Momentary affects were grouped as positive and negative affects. Data analyses were performed using mixed model and Pearson’s correlation analyses.
Results:
Independently of group, individuals reporting more positive affect reported significantly lower pain intensity. Individuals in the primary pain group tended to report higher pain intensity compared to the secondary pain group. Exploratory analyses indicated significant correlations of pain intensity and depressive symptoms, and of pain intensity and affects only in the group with primary pain.
Conclusions:
Despite their preliminary nature due to the small sample size, our findings indicate a general beneficial effect of positive momentary affects on pain intensity in daily life. They also provide first evidence for differences in the perception of pain intensity between primary and secondary chronic pain patients with regard to the relationship between pain intensity and affects.
Commensal adaptation of yeast Saccharomyces cerevisiae to grape-skin environment
Authors: Watanabe Daisuke , Hashimoto Wataru 
Published: March 2023

Abstract
Saccharomyces cerevisiae, an essential player in alcoholic fermentation during winemaking, is rarely found in intact grapes. Although grape-skin environment is unsuitable for S. cerevisiae’s stable residence, S. cerevisiae or its relatives can increase population on grape berries after colonization during raisin production. Here, we addressed commensal adaptation of S. cerevisiae to grape-skin ecosystem. The yeast-like fungus Aureobasidium pullulans, a major grape-skin resident, exhibited broad spectrum assimilation of plant-derived carbon sources, including ω-hydroxy fatty acid, arising from degradation of plant cuticles. In fact, A. pullulans encoded and expressed esterase for cuticle degradation. When intact grape berries were used as a sole carbon source, non-fermentative A. pullulans or other residents accelerated S. cerevisiae’s alcoholic fermentation via increasing carbon availability. Thus, degradation and utilization of grape-skin materials by resident microbiota may account for their indigenous and S. cerevisiae’s commensal behaviors. Such plant-microbe symbiotic interaction may be a prerequisite for triggering spontaneous food fermentation.
HuR/miR-124-3p/VDR complex bridges lipid metabolism and tumor development in colorectal cancer
Authors: Huang Fengxing , Bu Luping , Li Mengting , Wang Youwei , Zhang Runan , Shao Yu , Lin Kun , Yang Hong , Zhao Qiu , Liu Lan 
Published: March 2023

AbstractBackground:Maintaining lipid homeostasis to prevent lipotoxicity is crucial for various tumors, including colorectal cancer (CRC). Hu-antigen R (HuR) is a member of the RNA binding protein family and overexpressed in many cancer types, which implicted that in regulating cell proleferation, migration, invasion, and lipid metabolism. However, the role of HuR in regulating abnormal lipid metabolism of CRC is unknown.Methods:Western blot was performed to screen differentially expressed HuR between CRC tissues and adjacent normal tissues. Lipidomic profiling, RNA sequencing (RNA-seq), Cell Counting Kit-8 (CCK-8), total cholesterol and triglycerides assays testified the critical role of HuR/miR-124-3p/VDR complex in CRC cells. RNA pull-down and luciferase reporter Assays were performed to verify the interaction between HuR protein and the VDR mRNA. We also conducted a mouse xenograft model to elucidate the effect of HuR on lipid homeostasis and proliferation in vivo.Results:Our study identified that HuR promotes the expression of VDR, then modulates lipid homeostasis by enhancing TG and TC levels in CRC. Here, our study demonstrated that overexpressing HuR enhanced the expression of VDR through directly binding to its CDS and 3’-UTR. Simultaneously, HuR also indirectly affecting VDR by inhibiting miR-124-3p. We identified that HuR can suppress the expression of miR-124-3p, while miR-124-3p can bind to 3’-UTR of VDR to inhibit the expression of VDR. Moreover, xenograft models showed that targeting HuR suppressed the expression of VDR, blocked TG and TC formation, then suppressed CRC growth.Conclusion:Our findings propose a regulatory connection between HuR, miR-124-3p and VDR in CRC cells. We suggested that HuR/miR-124-3p/VDR complex modulates lipid homeostasis by influencing TG and TC formation in CRC, and may provide a potential target for CRC treatment and prevention.