bluuebunny/crossref_metadata_2025_split huggingface dataset:
SF3B4 Regulated Alternative Splicing that Produced an Oncogenic Isoform of HOXA11-AS to Promote Glycolytic Reprogramming and ESCC Progression
Authors: li xiaoya , Dai Suli , Wei Sisi , Zhang Cong , Shan Changliang , Liu Yueping , Lv Huilai , Shan Baoen , Zhao Lianmei 
Published: July 2023

Abstract
Background
Dysregulated splicing factors (SFs) and aberrant alternative splicing (AS) events are involved in tumor progression. However, the AS landscape underlying SFs dysregulation and the further signal transduction network were unraveled in esophageal squamous cell carcinoma (ESCC). This study revealed the biological function of splicing factor 3b subunit 4 (SF3B4) in non-coding RNA AS and glycolytic reprogramming, and proposed a novel diagnostic pannel and therapeutic targets for ESCC.
Methords
The expression, diagnostic efficiency and prognostic value of SF3B4 were investigated by bioinformatics, real-time fluorescent quantitative PCR and immunohistochemistry assays. The biological functions of SF3B4 in ESCC were analyzed in vivo and in vitro by loss-of-function studies. RNA sequencing, minigene reporter, RNA immunoprecipitation and correlation analysis were performed to elucidate SF3B4-regulated AS isoforms and SF3B4-interaction motif. Seahorse metabolism assays and high performance liquid chromatography-mass spectrometry analysis were conducted to explore the potential molecular mechanism of SF3B4 and downstream AS isoforms in driving ESCC development.
Results
SF3B4 was significantly up-regulated in ESCC and facilitated cell proliferation, survival, cycle progression and cisplatin resistance. Mechanically, SF3B4 increased proportion of the tumorigenic splicing isoform (HOX-L) of long noncoding RNA homeobox A11 antisense (HOXA11-AS), which resulted in enhanced glycolysis and elevated transcription of glycolytic enzyme PKM2, ENO1, HK2, GLUT1, LDHA and PGK1, through promoting phosphorylation of β-catenin at serine 675 and activation of Wnt pathway. Remarkably, inhibition of glycolysis reversed the malignant phenotype induced by the SF3B4-HOX-L axis. Moreover, the RNA levels of SF3B4 and HOX-L were positively correlated with ESCC tumor volume, and high SF3B4 expression demonstrated significant poor survival for ESCC patients. Additionally, the combination of SF3B4 and HOXA11-AS expression also showed good diagnostic performance.
Conclusions
These findings highlighted the oncogenic role of the SF3B4-HOX-L- Wnt-β-catenin-glycolytic enzyme axis in ESCC development, and proposed SF3B4 and HOX-L splicing isoform as novel therapeutic targets and diagnostic biomarkers for ESCC.
Morphology Analysis of Descending Tracts in the Mouse Spinal Cord Using Tissue Clearing, Tissue Expansion and Tiling Light Sheet Microscopy Techniques
Authors: Xie Jiongfang , Feng Ruili , Chen Yanlu , Gao Liang 
Published: July 2023

Abstract
Descending tracts are the pathways to send motor signals from the brain to spinal cord playing an important role in movement coordination. However, there are rare reports to show the full view of the tracts with a long span projection and variety axon morphologies from 3D perspective. Herein, five less-studied descending tracts in the mouse spinal cord were analyzed that originate from midbrain, cerebellum or brainstem individually by the tissue clearing method in combination with tiling light sheet microscopy. By tracing dozens of axons in spinal cord, we reported several novel findings of the different tracts: the collateral “sister” branches occurred only in the axons originating from the cerebellospinal tracts among these five tracts; the axons from superior colliculus terminated only at upper cervical but with abundant branches in the brainstem; the SP5 (spinal trigeminal nucleus) axons crossed the midline of spinal cord to contralateral while the MdV (medullary reticular formation, ventral part) axons had plenty of branches in both cervical and lumbar segments. The characteristics of these branch features in different tracts hinted the function of specific descending pathway. Furthermore, we preliminarily investigated the synaptic connection between the tracts and motor neurons in the spinal cord through hydrogel-based tissue expansion and found several synaptic connections between MdV axons and motor neurons. The present study, for the first time, described the full-view of the projection patterns and axon morphologies of five descending tracts in the mouse spinal cord under 3D view, which was essential for better understanding the function of these descending tracts. Meanwhile, we developed a new method for future study of descending tracts by three-dimensional imaging.
Future Land Use Land Cover Scenario Simulation Using Open-Source GISFor The Saroor Nagar Watershed, Telangana, India
Authors: Vaddiraju Shiva Chandra , Talari Reshma , K Bhavana , S Apsana 
Published: July 2023

Abstract
The dynamics of land use and land cover are profoundly affected by the growth, mobility, and demand of people. Thematic maps of land use and land cover (LULC) help planners account for conservation, concurrent uses, and land-use compressions by providing a reference for analysis, resource management, and prediction. The purpose of this research is to identify the transition of land-use changes in the Saroor Nagar Watershed between 2008 and 2014 using the MOLUSCE plugin (MLP-ANN) model and to forecast and establish potential land-use changes for the years 2020 and 2026. To predict how these factors affected LULC from 2008 to 2014, MLP-ANN was trained with maps of DEM, slope, distance from the road, and distance to a waterbody. The projected and accurate LULC maps for 2020 have a Kappa value of 0.70 and a correctness percentage of 81.8%, indicating a high degree of accuracy. Changes in LULC are then predicted for the year 2026 using MLP-ANN, which shows a 17.4% increase in built-up area at the expense of vegetation and barren land. The results contribute to the development of sustainable plans for land use and resource management.
Sphingosine d18:1 Promotes Nonalcoholic Steatohepatitis by Inhibiting Macrophage HIF-2α
Authors: Jiang Changtao , Xia Jialin , Chen Hong , Wang Xiaoxiao , Chen Weixuan , Lin Jun , Xu Feng , Nie Qixing , Ye Chuan , Zhong Bitao , Zhao Min , Yun Chuyu , Zeng Guangyi , Yan Sen , Wang Xuemei , Sun Lulu , Liu Feng , Rao Huiying , Pang Yanli 
Published: July 2023

Abstract

Non-alcoholic steatohepatitis (NASH) is a severe type of the non-alcoholic fatty liver disease (NAFLD). NASH is a growing global health concern due to its increasing morbidity, lack of well-defined biomarkers and lack of clinically effective treatments. Using metabolomic analysis, the most significantly changed active lipid sphingosine d18:1 [So(d18:1)] was selected from NASH patients. So(d18:1) inhibits macrophage HIF-2α as a direct inhibitor and promotes the activation of NLRP3 inflammasome. Macrophage-specific HIF-2α knockout and overexpression mice verified the effect of HIF-2α on NASH progression. Importantly, the HIF-2α stabilizer FG-4592 alleviated liver inflammation and fibrosis in NASH, which indicated that macrophage HIF-2α was a potential drug target for NASH treatment. Overall, this study confirms that So(d18:1) promotes NASH and clarifies that So(d18:1) inhibits the transcriptional activity of HIF-2α in liver macrophages by suppressing the interaction of HIF-2α with ARNT, suggesting that macrophage HIF-2α may be a new target for the treatment of NASH.
Interaction-driven spontaneous broken-symmetry insulator and metals in ABCA tetralayer graphene
Authors: Chen Guorui , Liu Kai , Zheng Jian , Sha Yating , Lyu Bosai , Li Fengping , Park Youngju , Ren Yulu , Watanabe Kenji , Taniguchi Takashi , Jia Jinfeng , Luo Weidong , Shi Zhiwen , Jung Jeil 
Published: July 2023

Abstract
Interactions among charge carriers in graphene can lead to the spontaneous breaking of multiple degeneracies. When increasing the number of graphene layers following rhombohedral stacking, the dominant role of Coulomb interactions becomes pronounced due to the significant reduction in kinetic energy. In this study, we employ phonon-polariton assisted near-field infrared imaging to determine the stacking orders of tetralayer graphene devices. Through quantum transport measurements, we observe a range of spontaneous broken-symmetry states and their transitions, which can be finely tuned by carrier density n and electric displacement field D. Specifically, we observe a layer antiferromagnetic insulator at n = D = 0 with a gap of approximately 15 meV. Increasing D allows for a continuous phase transition from a layer antiferromagnetic insulator to a layer polarized insulator. By simultaneously tuning nand D, we observe isospin polarized metals, including spin-valley-polarized and spin-polarized metals. These transitions are associated with changes in Fermi surface topology and are consistent with the Stoner criteria. Our findings highlight the efficient fabrication of specially stacked multilayer graphene devices and demonstrate that crystalline multilayer graphene is an ideal platform for investigating a wide range of broken symmetries driven by Coulomb interactions.
Study protocol for a prospective observational study to evaluate the efficacy of fosnetupitant for long-delayed chemotherapy-induced nausea and vomiting in patients receiving platinum- based chemotherapy (LODEC-N)
Authors: Iimura Yohei , Iihara Hirotoshi , Aoyama Takeshi , Ishibashi Masaaki , Sasuga Chieko , Furukawa Naoki , Anzai Eri , Ijichi Yuki , Takahashi Sayuri , Tabata Mariko , Niimi Fusako , Kaneko Jun , Izukuri Kazuyoshi , Baba Keisuke , Boku Narikazu , Kuroda Seiichiro 
Published: July 2023

Abstract
Background
The efficacy of fosnetupitant (FosNTP) in combination with palonosetron and dexamethasone for preventing highly emetogenic chemotherapy-induced nausea and vomiting (CINV) was demonstrated in a phase III study (CONSOLE study). Although the exploratory analysis of the CONSOLE study suggested the efficacy of triplet antiemetic therapy, including FosNTP, in the extended overall phase (0–168 h), its efficacy in the long-delayed phase (> 168 h) has not been evaluated. Additionally, the efficacy of FosNTPs in moderately emetogenic chemotherapy has not yet been elucidated. Therefore, this study aims to prospectively assess the efficacy of FosNTP for CINV in the long-delayed phase (> 168 h) in patients receiving platinum-based chemotherapy (cisplatin, carboplatin, and oxaliplatin).
Methods
This is a single-center, single-arm, prospective observational study. Patients scheduled to receive platinum-based chemotherapy will be enrolled. Clinical pharmacists and attending physicians will evaluate all adverse events. The primary endpoint is a long-delayed (120–336 h) complete control (CC) rate, defined as the proportion of patients experiencing no emetic episodes and no moderate or severe nausea without rescue medication. The main secondary endpoints include a long-delayed complete response (CR) rate, defined as the proportion of patients experiencing no emesis without rescue medication, and an overall (0–336 h) CC, CR, and total control rates, defined as the proportion of patients experiencing no vomiting and nausea without rescue medication in the extended overall phase (0–336 h). A subset analysis is planned according to the CINV risk of chemotherapy for each endpoint and time-to-treatment failure for each agent.
Discussion
This study aims to elucidate the efficacy of triplet antiemetic therapy, including FosNTP, and identify risk factors for CINV in the long-delayed phase in patients receiving platinum-based chemotherapy.
Trial Registration:
This trial was registered in the Japan Registry of Clinical Trials (jRCT) as jRCT1030230130.
Protocol version
V.1.0, March 19, 2023.
Landslide Hazard Mapping in Son La Province, Vietnam Under Climate Change Scenarios for 2025 &amp; 2050
Authors: Tuan Le Canh , Khanh Nguyen Quoc , Giang Nguyen Khac Hoang 
Published: July 2023

Abstract
In recent years, landslides in mountainous provinces are increasing. Whenever the rainy season comes, landslides occur, causing casualties to people and damages to the infrastructure. Son La, a mountainous province in the North of Vietnam, has suffered significant damage from landslides.
Therefore, the purpose of this research is to introduce an application of the analytic hierarchy process (AHP) method with the integration of representative concentration pathways (RCP) scenario 4.5 to study landslides in Son La province, Vietnam. With the integration of the digital elevation model (DEM) and collected data into ArcGIS software, we obtained different factor maps: Slope map, Aspect map, Cleavage density map, River density map, Rainfall map, Lithology map, Fault density map, Vegetation cover map, Distance to roads. These were the important input parameters for the AHP method. The data collected from 2009 to the present and the research documents of the authors based on the field investigations in 2018, 2019, 2020, and 2021 were the basis for us to complete this article. The landslide risk maps with the integration of maximum daily rainfall from the climate change scenario for 2025 and 2050 were produced. The results revealed that the landslide risk in Son La province increases with time. The results from this research will assist local authorities in land use planning, disaster control, and achieving sustainable development goals.
Conserved transcriptomic signatures and protein markers in cellular senescence models
Authors: Skea Dionysia , Fotis Christos , Tsolakos Nikos , Pliaka Vicky , Verrou Kleio-Maria , Alexopoulos Leonidas G. 
Published: July 2023

Abstract
Cellular senescence is described as an irreversible cell cycle arrest induced in response to various stresses. Senescent cells are characterised by heterogeneous signalling alterations, complex secretory phenotype, known as senescence-associated secretory phenotype (SASP), and diverse transcriptomic profile. With the aim to investigate senescence heterogeneity and identify conserved transctiptomic alterations and senescence markers, we performed RNA-seq and multiplex proteomic analysis in proteasome inhibition-induced and stress-induced premature senescence models of HFL1 and BJ human fibroblasts. Our data revealed diverse transcriptomic signatures, but also, 231 common differentially expressed genes related to cell division and ECM remodelling, and enriched pathways that remained conserved among the different models with senescence onset. Moreover, we identified a subset of conserved protein senescence markers and validated them in replicative senescent models. These proteins are involved in cell cycle arrest and promote a pro-inflammatory environment in premature and replicative senescence models. We suggest that the simultaneous analysis of p21, p-c-JUN, BCL-xL and survivin in cellular lysates, and IL-8, GM-CSF, GDF-15 and GROa in culture supernatants can provide a powerful tool for the identification and monitoring of senescent cells and can support the assessment of the efficacy of potential senotherapeutic approaches.
Reducing the rate of central line-associated bloodstream infections; a quality improvement project
Authors: Odada David , Munyi Hellen , Gatuiku Japhet , Thuku Ruth , Nyandigisi Jared , Wangui Anne , Ashihundu Emilie , Nyakiringa Beatrice , Kimeu Jemimah , Musumbi Martin , Adam Rodney 
Published: July 2023

Abstract
Background
The burden of central line-associated bloodstream infections is significant and has negative implications for healthcare, increasing morbidity and mortality risks, increasing inpatient hospital stays, and increasing the cost of hospitalization. Efforts to reduce the incidence of central line-associated bloodstream infections have utilized quality improvement projects that implement, measure, and monitor outcomes. However, variations in location, healthcare organization, patient risks, and practice gaps are key to the success of interventions and approaches. This study aims to evaluate interventions of a quality improvement project on the reduction of central line-associated bloodstream infection rates at a university teaching hospital.
Methods
This was a retrospective review of a quality improvement project that was implemented using the Plan-Do-Study-Act quality improvement cycle. Active surveillance of processes and outcomes was performed in the critical care areas; compliance to central line care bundles, and central line-associated bloodstream infections. Interrupted time series was used to analyze trends pre and post-intervention and regression modeling to estimate data segments preceding and succeeding the interventions.
Results
There were 350 central line insertions, 3912 catheter days, and 20 central line-associated bloodstream infection events during the intervention period. Compliance with central line care bundles was at 94%. There was a trend in the reduction of central line-associated bloodstream infections by 18% that did not reach statistical significance (p = 0.252).
Conclusions
Improvement projects to reduce central line-associated bloodstream infections face challenges and complexities associated with implementing interventions in real-world healthcare settings. There is a great need to continuously monitor, evaluate, readjust, and adapt interventions to achieve desired results, sustain improvements in patient outcomes, and investigate reasons for non-adherence as keys to achieving desired outcomes.
Empirical Ground-Motion Relations of peak ground acceleration in the region of the Gulf of California
Authors: Villalobos-Escobar Gina Paola , Castro Raúl R. , Peña-Alonso Tomás A. 
Published: July 2023

Abstract
We present a Ground Motion Prediction Equation (GMPE) of peak ground acceleration (PGA) for the Gulf of California (GoC), Mexico derived from PGA values of 720 earthquakes recorded between 2015 and 2018 by the Red Sismológica de Banda Ancha del Golfo de California (RESBAN), operated by the Centro de Investigación Científica y de Educación Superior de Ensenada, Baja California (CICESE).
We evaluated and adjusted two different parametric forms of GMPE developed for transcurrent tectonic regimes that resemble the tectonic setting in the GoC, along with an additional parametric form developed for central Mexico that incorporates the focal depth as an independent variable. All three adjustments exhibit appropriate behavior in terms of residual analysis and variables stability, with the parametrical form of Joyner and Boore (1981) providing the best overall performance.
The proposed GMPE is:
[[EQUATION]]
Where PGA is peak horizontal acceleration in cm/s2, M is magnitude and d is epicentral distance. [[EQUATION]]A = 0.18 and [[EQUATION]]E = 0.4 correspond to the intra-event and inter-event standard deviations respectively.
While the inclusion of focal depth as an independent variable has improved the adjustments in regions with different tectonic regimes, its incorporation did not provide any specific advantage in this case. The depth of the events was considered by incorporating a fixed value representing the average focal depth into the distance definition (11.86 km). This approach proved effective in capturing the seismic characteristics of the studied region, highlighting the importance of considering regional-specific factors when developing and refining GMPEs.
Outcomes and complications of prophylactic incisional gastropexy in 766 dogs (2009-2019)
Authors: Vega Miranda de la , Ralphs S. Christopher 
Published: July 2023

Abstract

Objective To report the outcomes and complications associated with prophylactic incisional gastropexy performed in dog breeds at risk for GDV. Results Seven hundred and sixty-six dogs underwent prophylactic incisional gastropexy of which 61 were electively performed at the time of castration or spay and 705 were adjunctively performed at the time of emergency abdominal surgery. All dogs had short-term follow-up, and 446 dogs (58.2%) had additional follow-up with a median long-term follow-up time of 876 days (range 58-4450). Only 3 dogs (0.4%) had a direct complication associated with the gastropexy site including hemorrhage causing hemoabdomen (2) and infection with partial dehiscence (1). No dogs with long-term follow-up experienced gastric dilatation (GD), gastric dilatation volvulus (GDV), or persistent GI signs following gastropexy. Results of this study found that complications directly associated with prophylactic gastropexy were rare and limited to hemorrhage causing hemoabdomen and infection with partial dehiscence. Transient postoperative GI signs may occur. Gastropexy malpositioning and bowel entrapment were not encountered. There was no occurrence of GD or GDV.
A novel primary antibiotic cement-coated locking plate as a temporary fixation for the treatment of open tibial fracture
Authors: Kang Yongqiang , Wu Yongwei , Ma Yunhong , Jia Xueyuan , Zhang Mingyu , Zhou Ming , Lin Fang , Rui Yongjun 
Published: July 2023

Abstract
Despite advances in Masquelet techniques of bone transfer, complex lower extremity trauma reconstruction remains a challenge. Herein, we present the use of a modified version of the Acute Masquelet technique to treat Grade III open fractures of the lower extremity. This modification can reduce the treatment period and complications of external fixation. We aimed to assess the outcomes of this technique for open fractures with bone and soft tissue defects. Between January 2018 and March 2021, 20 patients with Gustilo grade IIIB/C open fractures were treated with an antibiotic cement-coated locking plate as a temporary internal fixator during the initial surgery stage. Thorough debridement was performed in the emergency department, and temporary internal fixation was performed with a 3.5-mm system antibiotic cement-coated locking plate. Ten patients required free bone fragment removal, followed by bone cement packing. The final stage involved internal fixation and wound repair with a free anterolateral thigh flap. Clinical and imaging results were retrospectively analysed. The repair time ranged from 1 to 7 days. All flaps survived. Two patients experienced wound infection, and one developed severe bone infection 3 months after three-stage bone graft surgery. Autologous cancellous bone grafting was performed on ten patients with bone defects 6 weeks after surgery. Bone union was universally achieved after 1 year. This method proved to be safe and effective, with the repair of Grade III open fractures of the lower extremity achieved 1 to 7 days after treatment.