bluuebunny/crossref_metadata_2025_split huggingface dataset:
Characterization and Assessment of Natural Amazonian Clays for Cosmetics-Industry Applications
Authors: Kieling Antonio Claudio , Silva Cláudia Cândida , Júnior Sérgio Duvoisin , de Macedo Neto José Costa , de Oliveira Melquíades Miécio , del Pino Gilberto Garcia , de Souza Moreira Yago Ono , Panzera Túlio Hallak , da Silva Valenzuela Maria das Graças , Diáz Francisco Rolando Valenzuela 
Published: January 2023

AbstractClays are abundant materials in the Amazon region and have been used historically by ancient Amazonian people to produce ceramic and cosmetics products. The current study aimed to evaluate the potential of four clays from the metropolitan area of Manaus, each with a different color, for cosmetics applications. Two clays were collected in the Ponta Negra region (red and gray in color) in Manaus, one in Careiro (white), and one in Itacoatiara (black). After drying in an oven for 24 h at 105°C, the four clays were characterized by X-ray fluorescence (XRF), X-ray diffraction (XRD), infrared (IR) spectroscopy, thermogravimetry (TGA), differential thermal analysis (DTA), differential scanning calorimetry (DSC), scanning electron microscopy (SEM), particle-size analysis, and detection of microorganisms. The amounts of Al, Si, Cl, K, Ca, Ti, Cr, Fe, Zn, P, and S in all samples were below the limits for use in cosmetics. The main phases identified were kaolinite 1A, quartz, gibbsite, and the rare kaolinite 2M. Approximately 40 wt.% of each sample was in the < 20 μm particle-size range. Analyses by SEM revealed pseudo-hexagonal kaolinite structures with nano-islands and nanocrystallites. The low toxicity, mineralogic compositions, and particle-size findings suggest that Amazonian clays are promising for cosmetics applications.
Clinical application of circulating cell-free lymphoma DNA for fast and precise diagnosis of Burkitt lymphoma: Precision medicine for sub-Saharan Africa
Authors: Chamba Clara , Mbulaiteye Sam M. , Balandya Emmanuel , Schuh Anna 
Published: January 2023

Abstract
Burkitt lymphoma (BL) has a cure rate of around 95% when treated with chemo-immunotherapy that is standard of care in high-income countries (Minard-Colin et al., 2020, New England Journal of Medicine 382, 2207–2219), but currently, more than 50% of children and young adults with endemic BL (Epstein Barr virus driven BL) in sub-Saharan Africa (SSA) do not survive. Treatment for BL is largely free of charge, but there is limited access to reliable diagnostic services leading to significant delays and misdiagnoses. Innovations in histopathology such as whole slide imaging and the use of novel diagnostic approaches, in particular using circulating cell-free viral and/or lymphoma DNA (liquid biopsy), could increase access to timely and reliable diagnosis and improve outcomes in SSA.
ON THE SIZE, SPECTRAL RADIUS, DISTANCE SPECTRAL RADIUS AND FRACTIONAL MATCHINGS IN GRAPHS
Authors: LI SHUCHAO , MIAO SHUJING , ZHANG MINJIE 
Published: January 2023

AbstractWe first establish a lower bound on the size and spectral radius of a graph G to guarantee that G contains a fractional perfect matching. Then, we determine an upper bound on the distance spectral radius of a graph G to ensure that G has a fractional perfect matching. Furthermore, we construct some extremal graphs to show all the bounds are best possible.
Antonio Stoppani's ‘Anthropozoic’ in the context of the Anthropocene
Authors: Luciano Eugenio , Zanoni Elena 
Published: January 2023

AbstractThe figure of Antonio Stoppani (1824–91), an Italian priest, geologist and patriot, has re-emerged in the last decade thanks to discussions gravitating around the ‘Anthropocene’ – a term used to designate a proposed geological time unit defined and characterized by the mark left by anthropogenic activities on geological records. Among these discussions, Stoppani is often considered a precursor for popularizing the term ‘Anthropozoic’, which he used to describe and characterize the latest ‘era’ of Earth's geological time. His writings, largely unknown to an international audience before the ‘Anthropocene saga’, have been particularly in the spotlight after Valeria Federighi translated excerpts of his main geological work, Corso di Geologia, originally published in three volumes between 1871 and 1873. In the first edition of Corso di Geologia, namely Note ad un Corso Annual di Geologia, or simply Note, published between 1865 and 1870, Stoppani characterized the Anthropozoic in stratigraphic terms. In particular, Chapter 15 of the second volume of Note (1867) represents the first stratigraphic characterization that the author provides of the Anthropozoic. Our contribution here brings, for the first time, a translation of Chapter 15 to a broader international audience, accompanied by a critical commentary elucidating the broader social, political, religious and scientific context wherein the notion of Anthropozoic emerged in Stoppani's writings. The text of the original document and its translation can be found in the supplementary material tab at https://doi.org/10.1017/S0007087422000590.
Relationship between histopathological lesions and oxidative stress in mice infected with Angiostrongylus costaricensis
Authors: Zorzi N. R. , Benvegnú E. , Hermes C. , Rodriguez R. , Freddo N. , Webber B. , Amaral F. U. I. do , Scariot C. A. , Costa M. , Rossato-Grando L. G. , Vieira M. I. B. 
Published: January 2023

Abstract
This study describes changes in oxidative stress (OS) parameters in mice experimentally infected with Angiostrongylus costaricensis, which causes abdominal angiostrongyliasis. For this, 28 Swiss mice were used, divided into two groups (G1 and G2), with 14 animals each. Of these, eight were infected with ten infective larvae each, by gavage, and six were used as a control group. Mice from G1 and G2 were euthanized at 14 days and 24 days post-infection, respectively. Tissue samples were used for histopathological analysis and blood (serum) samples were taken to assess the levels of proteins, non-protein thiols (NPTs) and nitric oxide (NO), from centrifugation and subsequent collection of aliquots of the supernatant. Among OS parameters, infected mice in both groups had higher NO levels than the control group, due to the presence of: eosinophil infiltrate in the liver and intestine; pancreatitis; and intestinal granuloma. However, the infected mice of both groups showed a reduction in the levels of NPTs, in relation to the control group, due to the presence of: eosinophilic infiltrate in the liver and intestine; and intestinal granuloma. Our results suggest that A. costaricensis infection has important effects on the intestine, liver and pancreas, and the analyses were performed from the tissue of these organs. The mechanisms for these changes are related to the decrease in the body's main antioxidant defences, as demonstrated by the reduction of NPTs, thus contributing to the development of more severe tissue damage. Thus, the objective of the present study was to evaluate the relationship between histopathological lesions and markers for OS.
Observed and Parameterized Roughness Lengths for Momentum and Heat Over Rough Ice Surfaces
Authors: van Tiggelen Maurice , Smeets Paul C. J. P. , Reijmer Carleen H. , van den Broeke Michiel R. , van As Dirk , Box Jason E. , Fausto Robert S. 
Published: January 2023

AbstractTurbulent heat fluxes, that is, the sensible heat flux and latent heat flux, are important sources/sinks of energy for surface melt over glaciers and ice sheets. Therefore, credible simulations of for example, future Greenland Ice Sheet mass loss need an accurate description of these fluxes. However, the parameterization of surface turbulent heat fluxes in climate models requires knowledge about the surface roughness lengths for momentum, heat and moisture, which are currently either unknown or tuned to indirect observations. In this study we take advantage of a large data set of eddy covariance observations acquired during multiple years and at multiple sites over the Greenland Ice Sheet. These in‐situ observations are used to develop an improved parameterization for the roughness length for momentum, and update the parameterization for the roughness lengths for heat and moisture over rough ice surfaces. The newly derived parameterizations are implemented in a surface energy balance model that is used to compute surface melt. Sensitivity experiments confirm the high sensitivity of surface melt to the chosen roughness length models. The new parameterization models the sensible heat flux to within 10 W m−2, and the cumulative ice ablation within 10% at three out of four sites. Two case studies demonstrate the important contribution of the turbulent heat fluxes to surface ablation. The presented roughness parameterizations can be implemented in climate models to improve the physical representation of surface roughness over rough snow and ice surfaces, which is expected to improve the modeled turbulent heat fluxes and thus surface melt.
Impact of the Low Wavenumber Structure in the Initial Vortex Wind Analyses on the Prediction of the Intensification of Hurricane Patricia (2015)
Authors: Zhong Quanjia , Wang Xuguang , Ding Ruiqiang , Lu Xu , Huang Yongjie , Duan Wansuo , Liu Lei 
Published: January 2023

AbstractEnsemble clustering analysis was performed to explore the role of the initial hurricane vortex‐scale wind structure in the prediction of the intensification of Hurricane Patricia (2015). Convection‐allowing ensemble forecasts were classified into spin‐down (SPD) and spin‐up (SPU) groups. Specifically, 10 members with an intensification rate >0 m/s and 10 members with an intensification rate <0 m/s for the first 6 hr were defined as the SPD and SPU members. The result showed that the tangential winds outside the inner‐core region in the SPD members were weaker compared to the SPU members. Additionally, the SPD members had a weaker inflow near the surface and a weaker outflow between the heights of 8 and 12 km than the SPU members. The SPU members showed more significant azimuthal asymmetry than the SPD members in the surface, tangential and radial winds. Wavenumber analysis showed that the low wavenumber components dominated the differences between the SPD and SPU members. Numerical experiments were conducted to test the hypothesis generated by the clustering analysis. It was found that the storm's maximum wind speed (MWS) intensified during the first 6 hr of the model forecast if only the low wavenumber structure in the SPU members was included in the initial conditions, whereas it decayed during the first 6 hr if only the low wavenumber structure in the SPD members was included. This result confirms that the low wavenumber structure of the initial wind analyses was important in predicting the intensity changes of Hurricane Patricia (2015).
Model to Scale Rain Attenuation Time Series With Link Elevation Angle for LEO Satellite Based Systems
Authors: Tomaz L. M. , Capsoni C. , Luini L. 
Published: January 2023

AbstractA model to scale time series of rain attenuation along an Earth‐to‐satellite path with variable elevation angle is proposed. Starting from the time series at the lowest elevation the algorithm scales up each sample to the elevation of interest while maintaining the space and time correlation of the rain process and by correctly reproducing the local long‐term rain attenuation statistics. Because of these peculiarities, the model represents a key element for the development of attenuation time series synthesizers for LEO satellite‐based applications, where the time decorrelation of the rain event during the quick spacecraft passage over the ground station (roughly 10 min) is quite limited. Moreover, the comparison with the simple geometric scaling (i.e., the cosecant law) carried out among the attenuation Complementary Cumulative Distribution Functions shows a definite improvement in the prediction accuracy, even more so when the elevation angle falls below 20°.
Micrococcus luteus-derived extracellular vesicles attenuate neutrophilic asthma by regulating miRNAs in airway epithelial cells
Authors: Sim Soyoon , Lee Dong-Hyun , Kim Kwang-sun , Park Hyeon Ju , Kim Yoon-Keun , Choi Youngwoo , Park Hae-Sim 
Published: January 2023

AbstractBacterial extracellular vesicles (EVs) have been shown to regulate various pulmonary diseases, but their functions in asthma remain uncertain. To demonstrate the clinical significance of Micrococcus luteus-derived EVs (MlEVs) in asthma, we enrolled 45 asthmatic patients (20 patients with neutrophilic asthma [NA], 25 patients with eosinophilic asthma [EA]) and 40 healthy controls (HCs). When the prevalence of IgG1 and IgG4 specific to MlEVs was evaluated in serum by ELISA, lower levels of MlEV-specific IgG4 (but not IgG1) were noted in asthmatic patients than in HCs. Among asthmatic patients, significantly lower levels of MIEV-specific IgG4 were noted in patients with NA than in those with EA. Moreover, there was a positive correlation between serum MlEV-specific IgG4 levels and FEV1 (%) values. In asthmatic C57BL/6 mice, MlEVs significantly attenuated neutrophilic airway inflammation by reducing the production of IL-1β and IL-17 in bronchoalveolar lavage fluid as well as the number of group 3 innate lymphoid cells (ILC3s) in lung tissues. To clarify the functional mechanism of MlEVs in NA, the effect of MlEVs on airway epithelial cells (AECs) and immune cells was investigated ex vivo. According to microarray analysis, MlEVs upregulated hsa-miR-4517 expression in AECs. Moreover, this miRNA could suppress IL-1β production by monocytes, resulting in the inhibition of ILC3 activation and neutrophil recruitment. These findings suggest that MlEVs could be a novel therapeutic agent for managing unresolved NA by regulating miRNA expression in AECs.
Potential for mercury methylation by Asgard archaea in mangrove sediments
Authors: Zhang Cui-Jing , Liu Yu-Rong , Cha Guihong , Liu Yang , Zhou Xin-Quan , Lu Zhongyi , Pan Jie , Cai Mingwei , Li Meng 
Published: January 2023

Abstract
Methylmercury (MeHg) is a potent neurotoxin that bioaccumulates along food chains. The conversion of MeHg from mercury (Hg) is mediated by a variety of anaerobic microorganisms carrying hgcAB genes. Mangrove sediments are potential hotspots of microbial Hg methylation; however, the microorganisms responsible for Hg methylation are poorly understood. Here, we conducted metagenomic and metatranscriptomic analyses to investigate the diversity and distribution of putative microbial Hg-methylators in mangrove ecosystems. The highest hgcA abundance and expression occurred in surface sediments in Shenzhen, where the highest MeHg concentration was also observed. We reconstructed 157 metagenome-assembled genomes (MAGs) carrying hgcA and identified several putative novel Hg-methylators, including one Asgard archaea (Lokiarchaeota). Further analysis of MAGs revealed that Deltaproteobacteria, Euryarchaeota, Bacteroidetes, Chloroflexi, and Lokiarchaeota were the most abundant and active Hg-methylating groups, implying their crucial role in MeHg production. By screening publicly available MAGs, 104 additional Asgard MAGs carrying hgcA genes were identified from a wide range of coast, marine, permafrost, and lake sediments. Protein homology modelling predicts that Lokiarchaeota HgcAB proteins contained the highly conserved amino acid sequences and folding structures required for Hg methylation. Phylogenetic tree revealed that hgcA genes from Asgard clustered with fused hgcAB genes, indicating a transitional stage of Asgard hgcA genes. Our findings thus suggest that Asgard archaea are potential novel Hg-methylating microorganisms and play an important role in hgcA evolution.
General transcription factor TAF4 antagonizes epigenetic silencing by Polycomb to maintain intestine stem cell functions
Authors: Säisä-Borreill Susanna , Davidson Guillaume , Kleiber Thomas , Thevenot Andréa , Martin Elisabeth , Mondot Stanislas , Blottière Hervé , Helleux Alexandra , Mengus Gabrielle , Plateroti Michelina , Duluc Isabelle , Davidson Irwin , Freund Jean-Noel 
Published: January 2023

AbstractTaf4 (TATA-box binding protein-associated factor 4) is a subunit of the general transcription factor TFIID, a component of the RNA polymerase II pre-initiation complex that interacts with tissue-specific transcription factors to regulate gene expression. Properly regulated gene expression is particularly important in the intestinal epithelium that is constantly renewed from stem cells. Tissue-specific inactivation of Taf4 in murine intestinal epithelium during embryogenesis compromised gut morphogenesis and the emergence of adult-type stem cells. In adults, Taf4 loss impacted the stem cell compartment and associated Paneth cells in the stem cell niche, epithelial turnover and differentiation of mature cells, thus exacerbating the response to inflammatory challenge. Taf4 inactivation ex vivo in enteroids prevented budding formation and maintenance and caused broad chromatin remodeling and a strong reduction in the numbers of stem and progenitor cells with a concomitant increase in an undifferentiated cell population that displayed high activity of the Ezh2 and Suz12 components of Polycomb Repressive Complex 2 (PRC2). Treatment of Taf4-mutant enteroids with a specific Ezh2 inhibitor restored buddings, cell proliferation and the stem/progenitor compartment. Taf4 loss also led to increased PRC2 activity in cells of adult crypts associated with modification of the immune/inflammatory microenvironment that potentiated Apc-driven tumorigenesis. Our results reveal a novel function of Taf4 in antagonizing PRC2-mediated repression of the stem cell gene expression program to assure normal development, homeostasis, and immune-microenvironment of the intestinal epithelium.
Biomechanical analysis analyzing association between bone mineral density and lag screw migration
Authors: Lee Hyeonjoon , Kim Soo Ah , Jo Sungmin , Jo Suenghwan 
Published: January 2023

AbstractA proximal femoral nail using a helical blade (HB) is commonly utilized to treat proximal femoral fracture but cut through failure of the lag screws is one of the devastating complications following the surgery. While controversial, one of the potential risk factors for cut through failure is poor bone strength which can be predicted by measuring bone mineral density (BMD). In this study, we performed a biomechanical test on the fractured femoral head to validate whether the indirectly measured BMD from the contralateral hip or that measured directly from the retrieved femoral head can elucidate the structural strength of the fractured femoral head and thereby can be used to predict migration of lag screws. Our result showed that directly measured BMD has a significant correlation with the HB migration on the osteoporotic femoral head. However, while the BMDs measured from the contralateral femoral neck or total hip is the most widely used parameter to predict the bone strength of the fractured femur, this may have limited usability to predict HB migration.
Insufficient adenosine-induced hyperemia is a major determinant of discordance between non-hyperemic pressure ratio and fractional flow reserve
Authors: Matsumoto Hidenari , Higuchi Satoshi , Tanaka Hideaki , Masaki Ryota , Kondo Seita , Tsujita Hiroaki , Shinke Toshiro 
Published: January 2023

AbstractAdenosine occasionally overestimates fractional flow reserve (FFR) values (i.e., insufficient adenosine-induced hyperemia), leading to low non-hyperemic pressure ratios (NHPR)–high FFR discordance. We investigated the impact of insufficient adenosine-induced hyperemia on NHPR–FFR discordance and the reclassification of functional significance. We measured resting distal-to-aortic pressure ratio (Pd/Pa) and FFR by using adenosine (FFRADN) and papaverine (FFRPAP) in 326 patients (326 vessels). FFRADN overestimation was calculated as FFRADN − FFRPAP. We explored determinants of low Pd/Pa − high FFRADN discordance (Pd/Pa ≤ 0.92 and FFRADN > 0.80) versus high Pd/Pa − low FFRADN discordance (Pd/Pa > 0.92 and FFRADN ≤ 0.80). Reclassification of functional significance was defined as FFRADN > 0.80 and FFRPAP ≤ 0.80. Multivariable analysis identified FFRADN overestimation (p = 0.002) and heart rate at baseline (p = 0.048) as independent determinants of the low Pd/Pa–high FFRADN discordance. In the low Pd/Pa–high FFRADN group (n = 26), papaverine produced a further decline in the FFR value in 21 vessels (81%) compared with FFRADN, and the reclassification was observed in 17 vessels (65%). Insufficient adenosine-induced hyperemia is a major determinant of the low resting Pd/Pa–high FFR discordance. Physicians should bear in mind that the presence of low NHPR–high FFR discordance may indicate a false-negative FFR result.
Variational autoencoder-based estimation of chronological age and changes in morphological features of teeth
Authors: Joo Subin , Jung Won , Oh Seung Eel 
Published: January 2023

AbstractThis study led to the development of a variational autoencoder (VAE) for estimating the chronological age of subjects using feature values extracted from their teeth. Further, it determined how given teeth images affected the estimation accuracy. The developed VAE was trained with the first molar and canine tooth images, and a parallel VAE structure was further constructed to extract common features shared by the two types of teeth more effectively. The encoder of the VAE was combined with a regression model to estimate the age. To determine which parts of the tooth images were more or less important when estimating age, a method of visualizing the obtained regression coefficient using the decoder of the VAE was developed. The developed age estimation model was trained using data from 910 individuals aged 10–79. This model showed a median absolute error (MAE) of 6.99 years, demonstrating its ability to estimate age accurately. Furthermore, this method of visualizing the influence of particular parts of tooth images on the accuracy of age estimation using a decoder is expected to provide novel insights for future research on explainable artificial intelligence.
Mitochondrial Ca2+ handling as a cell signaling hub: lessons from astrocyte function
Authors: Cabral-Costa João Victor , Kowaltowski Alicia J. 
Published: January 2023

Abstract
Astrocytes are a heterogenous population of macroglial cells spread throughout the central nervous system with diverse functions, expression signatures, and intricate morphologies. Their subcellular compartments contain a distinct range of mitochondria, with functional microdomains exhibiting widespread activities, such as controlling local metabolism and Ca2+ signaling. Ca2+ is an ion of utmost importance, both physiologically and pathologically, and participates in critical central nervous system processes, including synaptic plasticity, neuron–astrocyte integration, excitotoxicity, and mitochondrial physiology and metabolism. The mitochondrial Ca2+ handling system is formed by the mitochondrial Ca2+ uniporter complex (MCUc), which mediates Ca2+ influx, and the mitochondrial Na+/Ca2+ exchanger (NCLX), responsible for most mitochondrial Ca2+ efflux, as well as additional components, including the mitochondrial permeability transition pore (mtPTP). Over the last decades, mitochondrial Ca2+ handling has been shown to be key for brain homeostasis, acting centrally in physiopathological processes such as astrogliosis, astrocyte–neuron activity integration, energy metabolism control, and neurodegeneration. In this review, we discuss the current state of knowledge regarding the mitochondrial Ca2+ handling system molecular composition, highlighting its impact on astrocytic homeostasis.