bluuebunny/crossref_metadata_2025_split huggingface dataset:
Purchasing for high-quality care using National Health Insurance: evidence from Zambia
Authors: Osei Afriyie Doris , Masiye Felix , Tediosi Fabrizio , Fink Günther 
Published: April 2023

Abstract
Improving the quality of care is essential for progress towards universal health coverage. Health financing arrangements offer opportunities for governments to incentivize and reward improvements in the quality of care provided. This study examines the extent to which the purchasing arrangements established within Zambia’s new National Health Insurance can improve equitable access to high-quality care. We adopt the Strategic Purchasing Progress and the Lancet Commission for High-Quality Health Systems frameworks to critically examine the broader health system and the purchasing dimensions of this insurance scheme and its implications for quality care. We reviewed policy documents and conducted 31 key-informant interviews with stakeholders at national, subnational and health facility levels. We find that the new health insurance could boost financial resources in higher levels of care, improve access to high-cost interventions, improve care experiences for its beneficiaries and integrate the public and private sectors. Our findings also suggest that health insurance will likely improve some aspects of structural quality but may not be able to influence process and outcome measures of quality. It is also not clear if health insurance will improve the efficiency of service delivery and whether the benefits realized will be distributed equitably. These potential limitations are attributable to the existing governance and financial challenges, low investments in primary care and shortcomings in the design and implementation of the purchasing arrangements of health insurance. Although Zambia has made progress in a short span, there is a need to improve its provider payment mechanisms, and monitoring and accounting for a higher quality of care.
Hyperbolic Anderson Model 2: Strichartz Estimates and Stratonovich Setting
Authors: Chen Xia , Deya Aurélien , Song Jian , Tindel Samy 
Published: April 2023

Abstract
We study a wave equation in dimension $d\in \{1,2\}$ with a multiplicative space-time Gaussian noise. The existence and uniqueness of the Stratonovich solution is obtained under some conditions imposed on the Gaussian noise. The strategy is to develop some Strichartz-type estimates for the wave kernel in weighted Besov spaces, by which we can prove the well-posedness of an associated Young-type equation. Those Strichartz bounds are of independent interest.
The Impact of the COVID-19 Pandemic on Human–Computer Interaction Empirical Research
Authors: Costagliola Gennaro , De Rosa Mattia , Fuccella Vittorio , Tabari Parinaz 
Published: April 2023

Abstract
The purpose of this paper is to assess the effects that the COVID-19 pandemic caused in the human–computer interaction (HCI) research field. Specifically, we aim to investigate how the HCI empirical research methodology changed due to the restrictions caused by COVID-19. For this reason, we analyzed all the papers published in the 2021 edition of The ACM Conference on Human Factors in Computing Systems (CHI 2021), which is generally considered the premier international conference for the field of HCI. Through the analysis of CHI papers, we identified four main effects of the COVID-19 pandemic on HCI research: influence on participants; influence on apparatus; influence on experiment procedure; other influences. These effects are described in detail and broken down into additional subcategories. Moreover, papers on pandemic-related topics were also identified. In addition, we performed some comparisons with the previous and successive edition of the conference, and extended some analysis, e.g categorization, to CHI 2022 papers. The analysis found that 23% of CHI 2021 papers and 36% of CHI 2022 papers reported some influences of the pandemic, the most common being a change in the procedures researchers used to interact with participants in their studies, in most cases based on remote communication technologies.
Intravesical aminoglycoside instillations as prophylaxis for recurrent urinary tract infection: patient satisfaction, long-term safety and efficacy
Authors: Bilsen Manu P , van Uhm Janneke I M , Stalenhoef Janneke E , van Nieuwkoop Cees , Groenwold Rolf H H , Visser Leo G , Lambregts Merel M C 
Published: April 2023

AbstractBackgroundRecurrent urinary tract infections (UTIs) are common, especially in women. When oral antimicrobial prophylaxis is ineffective or not possible due to allergies or antimicrobial resistance, intravesical aminoglycoside instillations (IAIs) are a non-systemic alternative.ObjectivesTo assess treatment satisfaction, long-term safety and efficacy of IAIs for recurrent UTI.MethodsWe conducted a cohort study using data collected between January 2013 and June 2022 at the Leiden University Medical Center. Adult patients with recurrent UTI who received prophylactic IAI were eligible for inclusion. Treatment satisfaction was assessed through a survey. Data on serum aminoglycoside concentrations, cystoscopy results and number of recurrences were obtained through chart review. Number of recurrences and UTI characteristics were compared between patients on and off IAI using Poisson and logistic mixed effects models.ResultsForty-four patients were included (median follow-up time 976 days) and 323 UTIs occurred during follow-up. Overall treatment satisfaction was high (median 79.2/100). All but one patient had undetectable serum aminoglycoside levels and no malignancies were found on follow-up cystoscopy. IAI increased the time to first recurrence (102 days versus 36 days, P = 0.02), reduced the number of recurrences (rate ratio 0.75, 95% CI 0.56–0.99, P = 0.04) and the necessity for systemic antibiotics (OR 0.33, 95% CI 0.13–0.86, P = 0.02).ConclusionsIn patients with recurrent UTI, IAI was associated with high treatment satisfaction, and was found to be a safe and effective alternative to oral antimicrobial prophylaxis.
Validation of an HPLC–HR-MS Method for the Determination and Quantification of Six Drugs (Morphine, Codeine, Methadone, Alprazolam, Clonazepam and Quetiapine) in Nails
Authors: Buratti Erika , Cippitelli Marta , Mietti Gianmario , Scendoni Roberto , Froldi Rino , Cerioni Alice , Cingolani Mariano 
Published: April 2023

Abstract
Keratinized matrices, including nails, are among the most resistant matrices that can be analyzed in cases where remains are deeply decomposed and relatively non-invasive for living people. In order to exploit these new matrices in the search for exogenous substances, it is necessary to develop analytical technologies capable of achieving high levels of sensitivity. In this technical note, an easy method is presented for the simultaneous extraction and quantification of three narcotic substances (morphine, codeine and methadone), two benzodiazepines (BDZs) (clonazepam and alprazolam) and an antipsychotic (quetiapine) from nail matrix by analysis in ultra-high-performance liquid chromatography at high-resolution mass spectrometry. The method has been validated following the Standard Practices for Method Validation in Forensic Toxicology of the Scientific Working Group for Forensic Toxicology. Nail specimens from eight authentic postmortem (PM) cases and 13 living donor samples were extracted and analyzed. Of the eight PM samples, five resulted positive for at least one of the three substances searched. Ten of the 13 living donor specimens were positive for at least one of the targeted BDZs or quetiapine.
Interaction of FlhF, SRP-like GTPase with FliF, MS ring component assembling the initial structure of flagella in marine Vibrio
Authors: Fukushima Yuria , Homma Michio , Kojima Seiji 
Published: April 2023

Abstract
Vibrio alginolyticus forms a single flagellum at its cell pole. FlhF and FlhG are known to be the main proteins responsible for the polar formation of single flagellum. MS-ring formation in the flagellar basal body appears to be an initiation step for flagellar assembly. The MS-ring is formed by a single protein, FliF, which has two transmembrane (TM) segments and a large periplasmic region. We had shown that FlhF was required for the polar localization of Vibrio FliF, and FlhF facilitated MS-ring formation when FliF was overexpressed in Escherichia coli cells. These results suggest that FlhF interacts with FliF to facilitate MS-ring formation. Here, we attempted to detect this interaction using Vibrio FliF fragments fused to a tag of Glutathione S-transferase in E. coli. We found that the N-terminal 108 residues of FliF, including the first TM segment and the periplasmic region, could pull FlhF down. In the first step, signal recognition particle (SRP) and its receptor are involved in the transport of membrane proteins to target them, which delivers them to the translocon. FlhF may have a similar or enhanced function as SRP, which binds to a region rich in hydrophobic residues.
Bioinformatics-Led Identification of Potential Biomarkers and Inflammatory Infiltrates in Burn Injury
Authors: Niu Zehao , Wei Guoxing , Liang Hao , Wang Xin , Yang Wenjuan , Wei Gang , Guo Jiachang , Chen Yingen , Tao Ran , Niu Jun 
Published: April 2023

Abstract
Burn injury is a life-threatening disease with a poor prognosis. The immune change and underlying mechanisms remain largely unknown. Thus, this study aims to find potential biomarkers and analyze the immune infiltrates after burn injury. Gene expression data of burn patients were obtained from the Gene Expression Omnibus database. Key immune-related genes (IRGs) were screened by differential and least absolute shrinkage and selection operator (LASSO) regression analysis. Based on key IRGs, patients were divided into two clusters by consensus cluster analysis. Immune infiltration was analyzed by the single sample gene set enrichment analysis (GSEA) method and the immune score was calculated by the principal component analysis method. A nomogram model was constructed based on the calculated immune score and clinical features. Finally, the expression of screened key genes was validated by an external cohort and quantitative polymerase chain reaction experiment. Fifty-nine IRGs were differently expressed in burn patients. After LASSO regression analysis, 12 key genes remained, namely AZU1, OLR1, RNASE2, FGF13, NR1D2, NR2E1, TLR5, CAMP, DEFA4, PGLYRP1, CTSG, and CCR3. Then, patients were divided into two clusters. Immune infiltration analysis revealed that more immune cells were infiltrated and more pathways were activated in cluster A, in which patients showed high immune scores. Finally, a nomogram model was constructed and showed high accuracy and reliability. The expression pattern of 12 key genes in an external cohort and clinical samples was in accordance with the theoretical analysis results. In conclusion, this research elucidated the key role of immune response in burns and could be used as a guide for burn treatment.
Dominance versus Prestige Hierarchies: How Social Hierarchy Base Shapes Conspicuous Consumption
Authors: Desmichel Perrine , Rucker Derek D 
Published: April 2023

Abstract
Consumers are known to seek out and display conspicuous goods—items that are exclusive and signal wealth and high social standing. Though many factors can drive such conspicuous consumption, the present work looks at an unexplored element: whether consumers find themselves in a dominance versus prestige-based hierarchy. Dominance-based hierarchies encourage consumers to use threatening, assertive, and manipulative behaviors to navigate the hierarchy. In contrast, prestige-based hierarchies encourage consumers to show their talent, skills, and motivation to operate within the hierarchy. The present research reveals how these two main bases of hierarchy shape consumers’ consumption of conspicuous goods. Specifically, dominance, relative to prestige hierarchies, is proposed to foster greater social anxiety, which leads people to seek conspicuous goods as a means of psychological security. Consequently, dominance-based hierarchies are more likely to encourage conspicuous consumption relative to prestige-based hierarchies. Multiple experiments, which utilize real employees’ behaviors, experimental manipulations of hierarchy, as well as incentivize-compatible decisions, reveal that dominance-based hierarchies, relative to prestige-based hierarchies, increase consumers’ preference for conspicuous goods. Moreover, evidence for a psychological mechanism in the form of social anxiety is demonstrated via both mediation and moderation.
Tissue clearing to examine glioma complexity in 3 dimensions
Authors: Yang Xinyi , Ye Zhaoyu , Zhang Minglin , Ouyang Ye , He Dian , Wang Xinyue , Lai Hei Ming , Sun Haitao 
Published: April 2023

Abstract
In recent years, tissue clearing has revolutionized the way we view biological materials. This has resulted in considerable advances in neuropathology and brain imaging. Its application to gliomas has the potential to increase understanding of tumor architecture, reveal mechanisms of tumor invasion, and provide valuable insights into diagnostics and treatments. This review outlines numerous tissue-clearing applications and recent developments in glioma research and delineates the limitations of existing technology and potential applications in experimental and clinical oncology.
DAP3-mediated cell cycle regulation and its association with radioresistance in human lung adenocarcinoma cell lines
Authors: Sato Yoshiaki , Yoshino Hironori , Sato Kota , Kashiwakura Ikuo , Tsuruga Eichi 
Published: April 2023

Abstract
Mitochondria play important roles in the cellular response to various types of stress, including that triggered by ionizing radiation. We have previously reported that the mitochondrial ribosomal protein death-associated protein 3 (DAP3) regulates the radioresistance of human lung adenocarcinoma (LUAD) cell lines A549 and H1299. However, the underlying mechanism of this regulation remains to be elucidated. To this end, we have herein investigated the role of DAP3 in the cell cycle regulation after irradiation. Notably, the DAP3 knockdown attenuated the radiation-induced increase of the G2/M cell population. Furthermore, western blotting analysis has revealed that the DAP3 knockdown decreased the expression of proteins related to the G2/M arrest, such as those of the phosphorylated cdc2 (Tyr15) and the phosphorylated checkpoint kinase 1 (Ser296), in irradiated A549 cells and H1299 cells. Moreover, by using a chk1 inhibitor, we were able to demonstrate that chk1 is involved in the radiation-induced G2/M arrest in both A549 and H1299 cells. Notably, the chk1 inhibitor was able to enhance the radiosensitivity of H1299 cells, while both chk1 inhibitor-abolished G2 arrest and inhibition of chk2-mediated events such as downregulation of radiation-induced p21 expression were required for enhancing radiosensitivity of A549 cells. Collectively, our findings reveal a novel role of DAP3 to regulate G2/M arrest through pchk1 in irradiated LUAD cells and suggest that chk1-mediated G2/M arrest regulates the radioresistance of H1299 cells, whereas both the chk1-mediated G2/M arrest and the chk2-mediated events contribute to the radioresistance of A549 cells.
Coronary reoperation with a free internal mammary artery connected to the right coronary artery as an inflow site; a coronary-to-coronary bypass
Authors: Ushioda Ryohei , Shirasaka Tomonori , Yoongtong Dit , Sakboon Boonsap , Cheewinmethasiri Jaroen , Kamiya Hiroyuki , Arayawudhikul Nuttapon 
Published: April 2023

AbstractSecond-time coronary artery bypass grafting is sometimes technically challenging due to severe adhesion of the heart, difficulty of identifying target coronary arteries, advanced sclerosis of the ascending aorta and limited availability of graft vessels. Here we report a patient, in whom a coronary-to-coronary bypass grafting from the native right coronary artery to the left anterior descending artery using a free right internal mammary artery was used as a graft conduit.
Sense in sensitivity: difference in the meaning of photoperiod insensitivity between wheat and barley
Authors: Slafer Gustavo A , Casas Ana M , Igartua Ernesto 
Published: April 2023

Abstract
The description of long photoperiod sensitivity in wheat and barley is a cause of confusion for researchers working with these crops, usually accustomed to free exchange of physiological and genetic knowledge of such similar crops. Indeed, wheat and barley scientists customarily quote studies of either crop species when researching one of them. Among their numerous similarities, the main gene controlling the long photoperiod sensitivity is the same in both crops (PPD1; PPD-H1 in barley and PPD-D1 in hexaploid wheat). However, the photoperiod responses are different: (i) the main dominant allele inducing shorter time to anthesis is the insensitive allele in wheat (Ppd-D1a) but the sensitive allele in barley (Ppd-H1) (i.e. sensitivity to photoperiod produces opposite effects on time to heading in wheat and barley); (ii) the main ‘insensitive’ allele in wheat, Ppd-D1a, does confer insensitivity, whilst that of barley reduces the sensitivity but still responds to photoperiod. The different behaviour of PPD1 genes in wheat and barley is put in a common framework based on the similarities and differences of the molecular bases of their mutations, which include polymorphism at gene expression levels, copy number variation, and sequence of coding regions. This common perspective sheds light on a source of confusion for cereal researchers, and prompts us to recommend accounting for the photoperiod sensitivity status of the plant materials when conducting research on genetic control of phenology. Finally, we provide advice to facilitate the management of natural PPD1 diversity in breeding programmes and suggest targets for further modification through gene editing, based on mutual knowledge on the two crops.
The dynamics of H2A.Z on SMALL AUXIN UP RNAs regulate abscisic acid–auxin signaling crosstalk in Arabidopsis
Authors: Yin Chunmei , Sun Aiqing , Zhou Ying , Liu Kunpeng , Wang Pan , Ye Wenjing , Fang Yuda 
Published: April 2023

Abstract
Extreme environmental changes threaten plant survival and worldwide food production. In response to osmotic stress, the plant hormone abscisic acid (ABA) activates stress responses and restricts plant growth. However, the epigenetic regulation of ABA signaling and crosstalk between ABA and auxin are not well known. Here, we report that the histone variant H2A.Z-knockdown mutant in Arabidopsis Col-0, h2a.z-kd, has altered ABA signaling and stress responses. RNA-sequencing data showed that a majority of stress-related genes are activated in h2a.z-kd. In addition, we found that ABA directly promotes the deposition of H2A.Z on SMALL AUXIN UP RNAs (SAURs), and that this is involved in ABA-repression of SAUR expression. Moreover, we found that ABA represses the transcription of H2A.Z genes through suppressing the ARF7/19–HB22/25 module. Our results shed light on a dynamic and reciprocal regulation hub through H2A.Z deposition on SAURs and ARF7/19–HB22/25-mediated H2A.Z transcription to integrate ABA/auxin signaling and regulate stress responses in Arabidopsis.
Delmopinol hydrochloride inhibitsCampylobacter jejunion skinless poultry meat, stainless steel, and high-density polyethylene food contact surfaces
Authors: Waldron Calvin , Eifert Joseph , O'Keefe Sean , Williams Robert , Le Tuan 
Published: April 2023

AbstractDelmopinol hydrochloride (delmopinol) is a cationic surfactant that is effective for treating and preventing gingivitis and periodontitis. This study evaluated the effectiveness of delmopinol for reducing attachment of Campylobacter jejuni to chicken meat, stainless steel, and high-density polyethylene (HDPE). These test materials were spot-inoculated with a C. jejuni culture. After 10 min, samples were sprayed with 0.5% or 1.0% delmopinol, 0.01% sodium hypochlorite, or distilled water. After a 1, 10, or 20 min contact time, samples were rinsed, which were serially diluted onto Campy-Cefex Agar. For additional samples, solutions were applied before inoculation with C. jejuni. Cultures remained undisturbed for 1, 10, or 20 min. Samples were then rinsed and plated as above. When C. jejuni was inoculated before treatments, 1% delmopinol application led to mean log reductions of 1.26, 3.70, and 3.72 log cfu ml−1, greater than distilled water alone, for chicken, steel and HDPE, respectively. When C. jejuni was inoculated after spray treatments, 1% delmopinol reduced C. jejuni by 2.72, 3.20, and 3.99 mean log cfu ml−1 more than distilled water for chicken, steel and HDPE, respectively. Application of 1% delmopinol, resulted in a significantly (P &lt; .05) greater log reduction than a 0.01% sodium hypochlorite or distilled water application.
A workflow model for holistic data management and semantic interoperability in quantitative archival research
Authors: Fafalios Pavlos , Marketakis Yannis , Axaridou Anastasia , Tzitzikas Yannis , Doerr Martin 
Published: April 2023

Abstract
Archival research is a complicated task that involves several diverse activities for the extraction of evidence and knowledge from a set of archival documents. The involved activities are usually unconnected, in terms of data connection and flow, making difficult their recursive revision and execution, as well as the inspection of provenance information at data element level. This article proposes a workflow model for holistic data management in archival research: from transcribing and documenting a set of archival documents, to curating the transcribed data, integrating it to a rich semantic network (knowledge graph), and then exploring the integrated data quantitatively. The workflow is provenance-aware, highly recursive and focuses on semantic interoperability, aiming at the production of sustainable data of high value and long-term validity. We provide implementation details for each step of the workflow and present its application in maritime history research. We also discuss relevant quality aspects and lessons learned from its application in a real context.
Chemical Warfare in Ancient Greek Ionia: Mass Intoxication With Hecate’s Deleterium
Authors: Tsoucalas Gregory , Symvoulakis Emmanouil K , Michaleas Spyros N , Tzatzarakis Manolis N , Karamanou Marianna 
Published: April 2023

ABSTRACT
The rich land of Erythrae in the coast of Asia Minor attracted the attention of the Ionian king Codrus. An oracle demanded the presence of the murky deity Hecate for him to conquer the city. Priestess Chrysame was sent by Thessalians to set the strategy of the clash. The young sorceress poisoned a sacred bull who turned mad, later to be released toward the camp of Erythraeans. The beast was captured and sacrificed. In the feast that followed, all ate a piece of his flesh and went crazy, stimulated by the poison, an easy prey for the army of Codrus. The deleterium used by Chrysame is unknown, but her strategy shaped the origin of biowarfare.