bluuebunny/crossref_metadata_2025_split huggingface dataset:
“Single-use peripheral” vs “conventional” reaming in total hip arthroplasty: how to respect native centre of rotation and acetabular offset? A CT study
Authors: Viglietta Edoardo , Previ Leonardo , Giuliani Veronica , Rescigno Giulia , Gugliotta Yuri , Redler Andrea , Iorio Raffaele 
Published: August 2023

Abstract
Purpose
The respect of native hip offset represents a mainstay for satisfying results in total hip arthroplasty (THA). Historically, a great interest has been focused on restoration of femoral offset, while only in recent years, acetabular offset (AO) has been considered. The purpose of the current study was to compare the “single-use peripheral” reaming technique with the “conventional” one for the maintenance of the native COR of the hip and AO in patients undergoing to primary THA.

Methods
Eighty patients affected from primary hip osteoarthritis were prospectively enrolled in the study and were divided in two groups (Group A “single-use peripheral” and Group B “conventional” reaming technique). Pre- and post-operatively, AO, acetabular floor distance (AFd) and acetabular version (AV) were assessed through a CT scan. A comparison between groups for the radiological parameters, surgical time and complications was performed.

Results
The demographic data were similar in both groups. The complications rate and the AV did not differ statistically between groups. Group A presented a statistically significant shorter surgical time and lower variation between pre- and post-operative AO and AFd. Statistical significance was defined as p < 0.05.

Conclusions
The “single-use peripheral” reaming technique demonstrated to be more reliable in reproducing the native COR and AO of patients undergoing to primary THA than the “conventional” one. The operative time was significantly reduced, and it may lead to a reduction in the infection risk even though it was not observed in the current study. Further research could be useful to validate such findings and to assess clinical impact and long-term survival of the implant.
Combined cone-beam CT imaging and microsurgical dissection of cadaver specimens to study cerebral venous anatomy: a technical note
Authors: Krogager Markus E. , Dahl Rasmus H. , Poulsgaard Lars , Fugleholm Kåre , Sehested Tom , Mikkelsen Ronni , Tranum-Jensen Jørgen , Mathiesen Tiit I. , Benndorf Goetz 
Published: August 2023

Abstract
Purpose
Cadaver dissections and X-ray based 3D angiography are considered gold standards for studying neurovascular anatomy. We sought to develop a model that utilize the combination of both these techniques to improve current tools for anatomical research, teaching and preoperative surgical planning, particularly addressing the venous system of the brain.

Materials and methods
Seven ethanol-fixed human cadaveric heads and one arm were injected with a latex-barium mixture into the internal jugular veins and the brachial artery. After the ethanol-based fixation, specimens were scanned by high-resolution cone-beam CT and images were post-processed on a 3D-workstation. Subsequent, microsurgical dissections were performed by an experienced neurosurgeon and venous anatomy was compared with relevant 3D venograms.

Results
Latex-barium mixtures resulted in a homogenous cast with filling of the cerebral venous structures down to 150 μm in diameter. The ethanol-based preparation of the cadaveric brains allowed for near-realistic microsurgical maneuverability during dissection. The model improves assessment of the venous system for anatomical education and hands-on surgical training.

Conclusion
To our knowledge we describe the first preparation method which combines near-realistic microsurgical dissection of human heads with high-resolution 3D imaging of the cerebral venous system in the same specimens.
Environmental drivers of eukaryotic plankton and fish biodiversity in an Arctic fjord
Authors: Marques Virginie , Hassler Christel , Deiner Kristy , Meier Elias , Valentini Alice , Albouy Camille , Pellissier Loïc 
Published: August 2023

AbstractArctic fjords are experiencing rapid environmental shifts due to climate change, which may have significant impacts on marine biodiversity and ecosystem functioning. However, the impact of climate change on fjord biodiversity is difficult to quantify given the low accessibility and high cost to sample these areas. In this study, we sampled locations from inside to outside an Arctic fjord and used environmental DNA metabarcoding to assess how the biodiversity of fish and eukaryotic plankton communities relate with environmental conditions. We detected a total of 12 fish taxa and 872 MOTUs for eukaryotes and found marked gradients of temperature and salinity driven by the distance to the glacier at the terminal part of the fjord and depth. Eukaryotic richness was mainly associated with lower temperature and chlorophyll a. Moreover, co-inertia analyses showed a shared structure between eukaryotes assemblages and the environmental gradients. A partial association between eukaryote and fishes suggest a potential shared effect of environmental gradients among these taxa. Our findings provide a baseline for future studies to assess how these assemblages may be impacted by ongoing environmental changes and highlight how fjord ice loss and warming might shift environmental gradients and species distribution under climate change.
Investigating the impact of structured reporting on the linguistic standardization of radiology reports through natural language processing over a 10-year period
Authors: Vosshenrich Jan , Nesic Ivan , Boll Daniel T. , Heye Tobias 
Published: August 2023

Abstract
Objectives
To investigate how a transition from free text to structured reporting affects reporting language with regard to standardization and distinguishability.

Methods
A total of 747,393 radiology reports dictated between January 2011 and June 2020 were retrospectively analyzed. The body and cardiothoracic imaging divisions introduced a reporting concept using standardized language and structured reporting templates in January 2016.
Reports were segmented by a natural language processing algorithm and converted into a 20-dimension document vector. For analysis, dimensionality was reduced to a 2D visualization with t-distributed stochastic neighbor embedding and matched with metadata. Linguistic standardization was assessed by comparing distinct report types’ vector spreads (e.g., run-off MR angiography) between reporting standards. Changes in report type distinguishability (e.g., CT abdomen/pelvis vs. MR abdomen) were measured by comparing the distance between their centroids.

Results
Structured reports showed lower document vector spread (thus higher linguistic similarity) compared with free-text reports overall (21.9 [free-text] vs. 15.9 [structured]; − 27.4%; p < 0.001) and for most report types, e.g., run-off MR angiography (15.2 vs. 1.8; − 88.2%; p < 0.001) or double-rule-out CT (26.8 vs. 10.0; − 62.7%; p < 0.001). No changes were observed for reports continued to be written in free text, e.g., CT head reports (33.2 vs. 33.1; − 0.3%; p = 1).
Distances between the report types’ centroids increased with structured reporting (thus better linguistic distinguishability) overall (27.3 vs. 54.4; + 99.3 ± 98.4%) and for specific report types, e.g., CT abdomen/pelvis vs. MR abdomen (13.7 vs. 37.2; + 171.5%).

Conclusion
Structured reporting and the use of factual language yield more homogenous and standardized radiology reports on a linguistic level, tailored to specific reporting scenarios and imaging studies.

Clinical relevance
Information transmission to referring physicians, as well as automated report assessment and content extraction in big data analyses, may benefit from standardized reporting, due to consistent report organization and terminology used for pathologies and normal findings.

Key Points
• Natural language processing and t-distributed stochastic neighbor embedding can transform radiology reports into numeric vectors, allowing the quantification of their linguistic standardization.
• Structured reporting substantially increases reports’ linguistic standardization (mean: − 27.4% in vector spread) and distinguishability (mean: + 99.3 ± 98.4% increase in vector distance) compared with free-text reports.
• Higher standardization and homogeneity outline potential benefits of structured reporting for information transmission and big data analyses.
Comprehensive analysis of CPSF4-related alternative splice genes in hepatocellular carcinoma
Authors: Yuemaierabola Anwaier , Guo Jun , Sun Lili , Yeerkenbieke Buerlan , Liu Fuzhong , Ye Dilinaer , Zhai Xiaoyi , Guo Wenjia , Cao Yan 
Published: August 2023

Abstract
Background
An important stage in controlling gene expression is RNA alternative splicing (AS), and aberrant AS can trigger the development and spread of malignancies, including hepatocellular carcinoma (HCC). A crucial component of AS is cleavage and polyadenylation-specific factor 4 (CPSF4), a component of the CPSF complex, but it is unclear how CPSF4-related AS molecules describe immune cell infiltration in the total tumor microenvironment (TME).

Methods
Using RNA-sequencing data and clinical data from TCGA-LIHC from the Cancer Genome Atlas (TCGA) database, the AS genes with differential expression were found. The univariate Cox analysis, KM analysis, and Spearman analysis were used to identify the AS genes related to prognosis. Screening of key AS genes that are highly correlated with CPSF4. Key genes were screened using Cox regression analysis and stepwise regression analysis, and prognosis prediction models and the topography of TME cell infiltration were thoroughly analyzed.

Results
A model consisting of seven AS genes (STMN1, CLSPN, MDK, RNFT2, PRR11, RNF157, GHR) was constructed that was aimed to predict prognostic condition. The outcomes of the HCC samples in the high-risk group were considerably worse than those in the lower risk group (p < 0.0001), and different risk patient groups were formed. According to the calibration curves and the area under the ROC curve (AUC) values for survival at 1, 2, and 3 years, the clinical nomogram performs well in predicting survival in HCC patients. These values were 0.76, 0.70, and 0.69, respectively. Moreover, prognostic signature was markedly related to immune infiltration and immune checkpoint genes expression.

Conclusion
By shedding light on the function of CPSF4 and the seven AS genes in the formation and progression of HCC, this research analysis contributes to the development of more useful prognostic, diagnostic, and possibly therapeutic biomarkers.
Implementing precision oncology for sarcoma patients: the CCCLMUmolecular tumor board experience
Authors: Berclaz Luc M. , Burkhard-Meier Anton , Lange Philipp , Di Gioia Dorit , Schmidt Michael , Knösel Thomas , Klauschen Frederick , von Bergwelt-Baildon Michael , Heinemann Volker , Greif Philipp A. , Westphalen C. Benedikt , Heinrich Kathrin , Lindner Lars H. 
Published: August 2023

Abstract
Purpose
Due to poor outcomes and limited treatment options, patients with advanced bone and soft tissue sarcomas (BS/STS) may undergo comprehensive molecular profiling of tumor samples to identify possible therapeutic targets. The aim of this study was to determine the impact of routine molecular profiling in the setting of a dedicated precision oncology program in patients with BS/STS in a German large-volume sarcoma center.

Methods
92 BS/STS patients who received comprehensive genomic profiling (CGP) and were subsequently discussed in our molecular tumor board (MTB) between 2016 and 2022 were included. Patient records were retrospectively reviewed, and the clinical impact of NGS-related findings was analyzed.

Results
89.1% of patients had received at least one treatment line before NGS testing. At least one molecular alteration was found in 71 patients (82.6%). The most common alterations were mutations in TP53 (23.3% of patients), followed by PIK3CA and MDM2 mutations (9.3% each). Druggable alterations were identified, and treatment recommended in 32 patients (37.2%). Of those patients with actionable alterations, ten patients (31.2%) received personalized treatment and six patients did benefit from molecular-based therapy in terms of a progression-free survival ratio (PFSr) > 1.3.

Conclusion
Our single-center experience shows an increasing uptake of next-generation sequencing (NGS) and highlights current challenges of implementing precision oncology in the management of patients with BS/STS. A relevant number of patients were diagnosed with clinically actionable alterations. Our results highlight the potential benefit of NGS in patients with rare cancers and currently limited therapeutic options.
Acupuncture improves immunity and fatigue after chemotherapy in breast cancer patients by inhibiting the Leptin/AMPK signaling pathway
Authors: Li Jinxia , Fu Ruiyang , Guo Xiaoqing , Pan Zhongqiang , Xie Jingjun 
Published: August 2023

Abstract
Objective
Acupuncture has become a popular complementary treatment in oncology. This study is based on RNA-Seq transcriptome sequencing technology to investigate the molecular mechanisms underlying the effect of acupuncture-mediated regulation of the Leptin/AMPK signaling pathway on mitochondrial dysfunction-induced fatigue in breast cancer patients after chemotherapy.

Methods
Peripheral blood samples from 10 patients with post-operative chemotherapy for breast cancer were selected for transcriptome sequencing to screen the key molecular pathways involved in fatigue after chemotherapy in breast cancer patients. Besides, peripheral blood samples were collected from 138 post-operative chemotherapy patients with breast cancer to study the composite fatigue and quality of life scores. Flow cytometry was used to detect T lymphocyte subsets in peripheral blood-specific immune cells. In addition, a blood cell analyzer was used to measure peripheral blood leukocyte counts, and MSP-PCR was used to detect mitochondrial DNA mutations in peripheral blood leukocytes.

Results
Transcriptome bioinformatics analysis screened 147 up-regulated mRNAs and 160 down-regulated mRNAs. Leptin protein was confirmed as the key factor. Leptin was significantly higher in the peripheral blood of breast cancer patients who developed fatigue after chemotherapy. Acupuncture treatment effectively improved post-chemotherapy fatigue and immune status in breast cancer patients, suppressed the expression of Leptin/AMPK signaling pathway-related factor and leukocyte counts, and significantly reduced the rate of mitochondrial DNA mutations in peripheral blood leukocytes.

Conclusion
The Leptin/AMPK signaling pathway may be the key molecular pathway affecting the occurrence of fatigue after chemotherapy in breast cancer patients. Leptin may improve post-chemotherapy fatigue in breast cancer patients by activating AMPK phosphorylation and alleviating mitochondrial functional impairment.
Coefficients of Multilinear Forms on Sequence Spaces
Authors: Raposo Anselmo , Serrano-Rodríguez Diana M. 
Published: August 2023

AbstractThe investigation of regularity/summability properties of the coefficients of bilinear forms in sequence spaces was initiated by Littlewood in 1930. Nowadays, this topic has important connections with other fields of Pure and Applied Mathematics. In this paper we explore a regularity technique to obtain optimal parameters for several results in this framework, extending/generalizing theorems of Osikiewicz and Tonge (Linear Algebra Appl 331:1–9, 2001), Albuquerque et al. (J Funct Anal 266:3726–3740, 2016), Aron et al. (Linear Algebra Appl 531:399–422, 2017), Albuquerque and Rezende (Commun Contemp Math 20:1750087, 2018), Paulino (Rend Circ Mat Palermo 69:369–380, 2020), among others.
Molecular signature of excessive female aggression: study of stressed mice with genetic inactivation of neuronal serotonin synthesis
Authors: Strekalova Tatyana , Moskvin Oleg , Jain Aayushi Y. , Gorbunov Nikita , Gorlova Anna , Sadovnik Daria , Umriukhin Aleksei , Cespuglio Raymond , Yu Wing Shan , Tse Anna Chung Kwan , Kalueff Allan V. , Lesch Klaus-Peter , Lim Lee Wei 
Published: August 2023

AbstractAggression is a complex social behavior, critically involving brain serotonin (5-HT) function. The neurobiology of female aggression remains elusive, while the incidence of its manifestations has been increasing. Yet, animal models of female aggression are scarce. We previously proposed a paradigm of female aggression in the context of gene x environment interaction where mice with partial genetic inactivation of tryptophan hydroxylase-2 (Tph2+/− mice), a key enzyme of neuronal 5-HT synthesis, are subjected to predation stress resulting in pathological aggression. Using deep sequencing and the EBSeq method, we studied the transcriptomic signature of excessive aggression in the prefrontal cortex of female Tph2+/− mice subjected to rat exposure stress and food deprivation. Challenged mutants, but not other groups, displayed marked aggressive behaviors. We found 26 genes with altered expression in the opposite direction between stressed groups of both Tph2 genotypes. We identified several molecular markers, including Dgkh, Arfgef3, Kcnh7, Grin2a, Tenm1 and Epha6, implicated in neurodevelopmental deficits and psychiatric conditions featuring impaired cognition and emotional dysregulation. Moreover, while 17 regulons, including several relevant to neural plasticity and function, were significantly altered in stressed mutants, no alteration in regulons was detected in stressed wildtype mice. An interplay of the uncovered pathways likely mediates partial Tph2 inactivation in interaction with severe stress experience, thus resulting in excessive female aggression.
A cross-platform application for the ecological and remote assessment of memory impairment in aging: ECO-MEMORY
Authors: Bruni Francesca , Mancuso Valentina , Greci Luca , Arlati Sara , Cavallo Marco , Riva Giuseppe , Goulene Karine , Stramba-Badiale Marco , Pedroli Elisa 
Published: August 2023

AbstractThis work aims to present the first step of a creation of an instrument to assess memory deficits responding to the needs imposed by the inability to access clinical care, such as physical or geographical constraints or still limitations imposed during the pandemic era. The older population, who would benefit from these services, may be at risk as access to services that support psychological and neuropsychological needs, which are not considered essential, has frequently been restricted in recent years. Moreover, because deficits are commonly mistaken for the effects of physiological aging, the early signs of cognitive decline might be ignored. On these bases, we used the potential of 360-degree media to create an application for memory assessment without the physical presence of clinicians: ECO-MEMORY. Firstly, we developed the application and evaluated its usability. ECO-MEMORY is divided into four sections, each addressing a different memory task: recognizing objects and faces, learning a path, and creating an allocentric map. Thirteen older adults who used the tablet application provided usability data as well as qualitative feedback on their experience. After the performance, the System Usability Scale, the Senior Technology Acceptance Model, and the Independent Television Commission Sense of Presence were administered. We performed a qualitative analysis and descriptive statistics, which showed that ECO-MEMORY is a usable instrument. Also, it was enjoyable for users who generally accepted technology in their life. ECO-MEMORY may therefore offer a promising approach to memory evaluation by including real-world scenarios.
Integrated multiomics analysis of chromosome 19 miRNA cluster in bladder cancer
Authors: Ware Akshay Pramod , Satyamoorthy Kapaettu , Paul Bobby 
Published: August 2023

AbstractWith 46 microRNAs (miRNAs) embedded tandemly over a distance of ~100 kb, chromosome 19 microRNA cluster (C19MC) is the largest miRNA cluster in the human genome. The C19MC is transcribed from a long noncoding genomic region and is usually expressed simultaneously at a higher level. Hence, we performed an integrative multiomics data analysis to examine C19MC regulation, expression patterns, and their impact on bladder cancer (BCa). We found that 43 members of C19MC were highly expressed in BCa. However, its co-localization with recurrent copy number variation (CNV) gain was not statistically significant to implicate its upregulation. It has been reported that C19MC expression is regulated by a well-established CpG island situated 17.6 kb upstream of the transcription start site, but we found that CpG probes at this island were hypomethylated, which was not statistically significant in the BCa cohort. In addition, the promoter region of C19MC is strongly regulated by a group of seven transcription factors (NR2F6, SREBF1, TBP, GATA3, GABPB1, ETV4, and ZNF444) and five chromatin modifiers (SMC3, KDMA1, EZH2, RAD21, and CHD7). Interestingly, these 12 genes were found to be overexpressed in BCa patients. Further, C19MC targeted 42 tumor suppressor (TS) genes that were downregulated, of which 15 were significantly correlated with patient survival. Our findings suggest that transcription factors and chromatin modifiers at the promoter region may regulate C19MC overexpression. The upregulated C19MC members, transcription regulators, and TS genes can be further exploited as potential diagnostic and prognostic indicators as well as for therapeutic management of BCa.
Development and verification of a physiologically motivated internal controller for the open-source extended Hill-type muscle model in LS-DYNA
Authors: Martynenko Oleksandr V. , Kempter Fabian , Kleinbach Christian , Nölle Lennart V. , Lerge Patrick , Schmitt Syn , Fehr Jörg 
Published: August 2023

AbstractNowadays, active human body models are becoming essential tools for the development of integrated occupant safety systems. However, their broad application in industry and research is limited due to the complexity of incorporated muscle controllers, the long simulation runtime, and the non-regular use of physiological motor control approaches. The purpose of this study is to address the challenges in all indicated directions by implementing a muscle controller with several physiologically inspired control strategies into an open-source extended Hill-type muscle model formulated as LS-DYNA user-defined umat41 subroutine written in the Fortran programming language. This results in increased usability, runtime performance and physiological accuracy compared to the standard muscle material existing in LS-DYNA. The proposed controller code is verified with extensive experimental data that include findings for arm muscles, the cervical spine region, and the whole body. Selected verification experiments cover three different muscle activation situations: (1) passive state, (2) open-loop and closed-loop muscle activation, and (3) reflexive behaviour. Two whole body finite element models, the 50th percentile female VIVA OpenHBM and the 50th percentile male THUMS v5, are used for simulations, complemented by the simplified arm model extracted from the 50th percentile male THUMS v3. The obtained results are evaluated additionally with the CORrelation and Analysis methodology and the mean squared error method, showing good to excellent biofidelity and sufficient agreement with the experimental data. It was shown additionally how the integrated controller allows simplified mimicking of the movements for similar musculoskeletal models using the parameters transfer method. Furthermore, the Hill-type muscle model presented in this paper shows better kinematic behaviour even in the passive case compared to the existing one in LS-DYNA due to its improved damping and elastic properties. These findings provide a solid evidence base motivating the application of the enhanced muscle material with the internal controller in future studies with Active Human Body Models under different loading conditions.