bluuebunny/crossref_metadata_2025_split huggingface dataset:
Extensive prevalence and significant genetic differentiation of Blastocystis in high- and low-altitude populations of wild rhesus macaques in China
Authors: Yu Mengshi , Yao Yongfang , Xiao Hongtao , Xie Meng , Xiong Ying , Yang Shengzhi , Ni Qingyong , Zhang Mingwang , Xu Huailiang 
Published: March 2023

Abstract
Background
Blastocystis is a common intestinal protist with a wide range of hosts. Thus far, 38 subtypes have been identified. In recent years, wild animals have been confronted with habitat fragmentation as well as an increasing risk of zoonotic disease transmission due to human disturbance. Only limited data are available on Blastocystis infection and subtype distribution in wild rhesus macaques in China. The aim of the present study was to investigate the prevalence and genetic diversity of Blastocystis in nine wild rhesus macaque populations in China.

Methods
A total of 276 faecal samples were collected from five high-altitude populations (high-altitude group [HAG]; 2800–4100 m a.s.l.) and four low-altitude populations (low-altitude group [LAG]; 5–1000 m a.s.l) of rhesus macaques. PCR-based analysis, using a new primer pair for the amplification of a 1690-bp sequence of the small subunit ribosomal RNA (SSU rRNA) gene, was used for prevalence and genetic diversity analysis.

Result
Analysis of faecal samples revealed that Blastocystis infection was common in rhesus macaques, with an infection positivity rate of 80.1% (n = 221/276 samples). There was no significant difference (P = 0.121) in positivity rate between the LAG (84.3%) and HAG (76.8%). Overall, 33 haplotypes were obtained and classified into four subtypes (STs), of which three were potentially zoonotic subtypes (ST1, 29.7%; ST2, 16.7%; ST3, 31.9%) and one that was first identified in this study and named ST39 (12.0%). The STs were distributed differently among the rhesus macaque populations, except for ST3, which was found in all populations. Phylogenetic analyses revealed two major divergent clades of ST3 for the HAG and LAG. Genetic diversity analysis showed a high genetic diversity of ST3 (haplotype diversity: 0.846; nucleotide diversity: 0.014) in the rhesus macaques, but a high genetic differentiation (FST > 0.25) and a low gene flow (Nm = 0.09) between the HAG and LAG.

Conclusion
Our study, which is the first investigation on Blastocystis infection in multiple wild rhesus macaque populations in China, indicates a potential risk of zoonotic transmission of Blastocystis in the study areas. Blastocystis ST3 showed high genetic diversity in wild rhesus macaques and significant genetic differentiation between the HAG and LAG. Our results provide fundamental information on the genetic diversity and prevalence of Blastocystis in wild rhesus macaque populations.

Graphical Abstract
Cryptococcus neoformans, a global threat to human health
Authors: Zhao Youbao , Ye Leixin , Zhao Fujie , Zhang Lanyue , Lu Zhenguo , Chu Tianxin , Wang Siyu , Liu Zhanxiang , Sun Yukai , Chen Min , Liao Guojian , Ding Chen , Xu Yingchun , Liao Wanqing , Wang Linqi 
Published: March 2023

AbstractBackgroundEmerging fungal pathogens pose important threats to global public health. The World Health Organization has responded to the rising threat of traditionally neglected fungal infections by developing a Fungal Priority Pathogens List (FPPL). Taking the highest-ranked fungal pathogen in the FPPL,Cryptococcus neoformans, as a paradigm, we review progress made over the past two decades on its global burden, its clinical manifestation and management of cryptococcal infection, and its antifungal resistance. The purpose of this review is to drive research efforts to improve future diagnoses, therapies, and interventions associated with fungal infections.MethodsWe first reviewed trends in the global burden of HIV-associated cryptococcal infection, mainly based on a series of systematic studies. We next conducted scoping reviews in accordance with the guidelines described in the Preferred Reporting Items for Systematic Reviews and Meta-analyses extension for Scoping Reviews using PubMed and ScienceDirect with the keywordCryptococcus neoformans to identify case reports of cryptococcal infections published since 2000. We then reviewed recent updates on the diagnosis and antifungal treatment of cryptococcal infections. Finally, we summarized knowledge regarding the resistance and tolerance ofC. neoformansto approved antifungal drugs.ResultsThere has been a general reduction in the estimated global burden of HIV-associated cryptococcal meningitis since 2009, probably due to improvements in highly active antiretroviral therapies. However, cryptococcal meningitis still accounts for 19% of AIDS-related deaths annually. The incidences of CM in Europe and North America and the Latin America region have increased by approximately two-fold since 2009, while other regions showed either reduced or stable numbers of cases. Unfortunately, diagnostic and treatment options for cryptococcal infections are limited, and emerging antifungal resistance exacerbates the public health burden.ConclusionThe rising threat ofC. neoformansis compounded by accumulating evidence for its ability to infect immunocompetent individuals and the emergence of antifungal-resistant variants. Emphasis should be placed on further understanding the mechanisms of pathogenicity and of antifungal resistance and tolerance. The development of novel management strategies through the identification of new drug targets and the discovery and optimization of new and existing diagnostics and therapeutics are key to reducing the health burden.
Natural world heritage conservation and tourism: a review
Authors: Zhang Zhenzhen , Xiong Kangning , Huang Denghong 
Published: March 2023

Abstract
The trade-off and synergy between heritage conservation and tourism has become the focus of natural world heritage research. To gain a better understanding of the global researches on natural World Heritage conservation and tourism, we comprehensively reviewed relevant peer-reviewed research literature based on Web of Science (WOS) and China National Knowledge Infrastructure (CNKI). We find that (1) the theoretical research of natural heritage conservation and tourism has gone through a process from emphasizing the protection of heritage value to pursuing the synergy of heritage protection and tourism development; (2) the main research methods include investigation research methods, indirect research methods and experimental research methods; (3) "3S" technology (remote sensing, geographic information system, global positioning system), three-dimensional laser scanning technology, virtual reality (VR) technology, augmented reality (AR) technology, holographic projection technology and other modern technological means are applied to the protection and tourism development of natural properties; (4) the common coordinated development models include ecological science tourism, community participation in tourism, ecological compensation model, world heritage—buffer zone—surrounding areas coordinated protection model and so on. We analyzed the research progresses through (1) the theories proposed in the literature, (2) the main methods applied to address the issues on natural heritage conservation and tourism, (3) the technologies applied in the researches and (4) the coordinated models of heritage conservation and tourism. Furthermore, we put forward the following research prospects: (1) systematically explore the conservation methods and theories based on world heritage criteria; (2) formulate corresponding conservation systems and ecological restoration standards for different types of world heritage; (3) give full play to the complementary advantages of various research methods and reveal the mutual feedback mechanism between tourism and heritage conservation; (4) develop ecological restoration technology based on biodiversity restoration, establish radial ecological corridor, and expand the benign ecological environment of the properties to wider periphery; (5) build ecological compensation development models based on the perspective of heritage tourism and value realization of world heritage.
Anisotropy of impact ionization in WSe2 field effect transistors
Authors: Kang Taeho , Choi Haeju , Li Jinshu , Kang Chanwoo , Hwang Euyheon , Lee Sungjoo 
Published: March 2023

AbstractCarrier multiplication via impact ionization in two-dimensional (2D) layered materials is a very promising process for manufacturing high-performance devices because the multiplication has been reported to overcome thermodynamic conversion limits. Given that 2D layered materials exhibit highly anisotropic transport properties, understanding the directionally-dependent multiplication process is necessary for device applications. In this study, the anisotropy of carrier multiplication in the 2D layered material, WSe2, is investigated. To study the multiplication anisotropy of WSe2, both lateral and vertical WSe2 field effect transistors (FETs) are fabricated and their electrical and transport properties are investigated. We find that the multiplication anisotropy is much bigger than the transport anisotropy, i.e., the critical electric field (ECR) for impact ionization of vertical WSe2 FETs is approximately ten times higher than that of lateral FETs. To understand the experimental results we calculate the average energy of the carriers in the proposed devices under strong electric fields by using the Monte Carlo simulation method. The calculated average energy is strongly dependent on the transport directions and we find that the critical electric field for impact ionization in vertical devices is approximately one order of magnitude larger than that of the lateral devices, consistent with experimental results. Our findings provide new strategies for the future development of low-power electric and photoelectric devices.
Graphical Abstract
Evaluation of a real-time mobile PCR device (PCR 1100) for the detection of the rabies gene in field samples
Authors: Demetria Catalino , Kimitsuki Kazunori , Yahiro Takaaki , Saito Nobuo , Hashimoto Takehiro , Khan Sakirul , Chu Maria Yna Joyce , Manalo Daria , Mananggit Milagros , Quiambao Beatriz , Nishizono Akira 
Published: March 2023

Abstract
Background
The Philippines is ranked among the top countries with 200–300 annual deaths due to rabies. Most human rabies cases have been reported in remote areas, where dog surveillance is inadequate. Therefore, a strategy to effectively improve surveillance in remote areas will increase the number of detections. Detecting pathogens using portable real-time reverse transcription-polymerase chain reaction (RT-PCR) has the potential to be accepted in these areas. Thus, we aimed to develop an assay to detect the rabies virus (RABV) genome by combining the robust primer system LN34 with the PicoGene PCR1100 portable rapid instrument targeting RABV RNA (PCR1100 assay).

Methods
Procedures were optimised using an LN34 primer/probe set, KAPA3G Plant PCR Kit (KAPA Biosystems), FastGene Scriptase II (NIPPON Genetics), and an artificial positive control RNA.

Results
Positive control RNA showed an analytical limit of detection of 10 copies/µL without false positivity, generating results in approximately 32 min. Compared to dFAT or RT-qPCR using field samples, the sensitivity and specificity of the PCR1100 assay were 100%, and even lower copy numbers (approximately 10 copies/µL) were detected.

Conclusions
This study demonstrated that the developed assay can detect rabies RNA in field samples. Because dog-mediated rabies is endemic in remote areas, the rapidity, mobility, and practicality of the PCR1100 assay as well as the high sensitivity of the LN34 system make it an ideal tool for the confirmation of rabies in these areas.
Wild mushroom consumption in the P’urhépecha Plateau at Michoacán, México: social, ethnomycological and nutritional issues
Authors: Torres-Gómez Mariano , Gómez-Peralta Marlene , Vázquez-Marrufo Gerardo 
Published: March 2023

AbstractAmong Mesoamerican cultures, P’urhépecha settlers inhabiting Michoacán State in Central Mexico preserve ancestral tradition on mushrooms eating. Most mycological knowledge available for this ethnical group has been addressed in the Pátzcuaro’s Lake zone, whereas P’urhépecha communities in other cultural and ecological relevant geographic areas remain unstudied. Thus, this work describes the ethnomycological knowledge of a scarcely analyzed Arantepacua P’urhépecha community at Michoacán Plateau region. Through structured interviews and field collections, data on the use, knowledge, biomass extraction and nomenclature of wild mushrooms were obtained. A list of P’urhépecha names to design 16 wild mushrooms species and 11 fruitbody components is documented and studied community used Spanish popular names to describe 21 wild mushrooms species and 11 fruitbody structures. Most valuable consumed mushroom species for Arantepacua settlers belong to the Amanita, Boletus, Hypomyces and Ramaria genera. Gender roles on mushrooms collect and cooking, new terms in P’urhépecha dialect to refer the parts of a fruitbody and vegetative mycelium, as well as local recipes and nutritional relevance of the mushroom species consumed by studied community are addressed and discussed. The ethnomycological knowledge documented contributes new terms in P’urhépecha to name the parts of an agarical fruitbody, the mycelium and empirical knowledge about mycorrhizal associations. This was the first P’urhépecha ethnomycological study made outside the Lake Pátzcuaro basin.
The diagnostic accuracy of an inclusive three-window ultrasonography assessment for the rapid authentication of endotracheal tube position in RICU
Authors: Sayed Islam G. , Salama Suzan , Abdallah Marwa 
Published: March 2023

AbstractBackgroundAssurance of proper endotracheal tube (ETT) location is crucial immediately after intubation as undiagnosed esophageal intubation can be catastrophic. The primary purpose for this study is to determine the diagnostic accuracy of to evaluate the accuracy of an inclusive three-window ultrasonography assessment for the rapid authentication of endotracheal tube position in the intensive care unit (RICU) with reference to Co2monitors (capnography) (the gold standard technique), The study included 100 patients who needed emergency intubation in the ICU of Aswan university hospital. Upon entrance to ICU, intubations was done by the residents and collective bedside three-windows ultra-sonography (tracheal, lung, diaphragmatic ultra-sonography) was carried out instantly after intubation Subsequently, the correct position of the endotracheal tube was established by the resident investigator via the use a capnometer.ResultsWaveform capnography revealed endotracheal intubation in 80 cases (80%) and esophageal intubation in 20 cases (20%). However, trans-tracheal ultra-sonography (TUS) was able to detect endotracheal intubation in 78 cases (78%) and esophageal intubation in 22 cases (22%) patients. SLS detected only 17 esophageal intubations from 20 cases detected by CO2 detectors. DUS was able to detect proper endotracheal intubation in 77 cases (77%) and esophageal intubation in 23 cases (23%). However, it detected only 17 esophageal intubations from 20 cases detected by Co2detectors.ConclusionsUltra-sonography, as recently introduced practice for validation of correct endotracheal tube location has both high accuracy and safety profile and can be used as a primary authentication technique.Trial registrationNCT05747248
Long-Term Performance of an Image-Based Short-Term Risk Model for Breast Cancer
Authors: Eriksson Mikael , Czene Kamila , Vachon Celine , Conant Emily F. , Hall Per 
Published: March 2023

PURPOSE Image-derived artificial intelligence–based short-term risk models for breast cancer have shown high discriminatory performance compared with traditional lifestyle/familial-based risk models. The long-term performance of image-derived risk models has not been investigated. METHODS We performed a case-cohort study of 8,604 randomly selected women within a mammography screening cohort initiated in 2010 in Sweden for women age 40-74 years. Mammograms, age, lifestyle, and familial risk factors were collected at study entry. In all, 2,028 incident breast cancers were identified through register matching in May 2022 (206 incident breast cancers were found in the subcohort). The image-based model extracted mammographic features (density, microcalcifications, masses, and left-right breast asymmetries of these features) and age from study entry mammograms. The Tyrer-Cuzick v8 risk model incorporates self-reported lifestyle and familial risk factors and mammographic density to estimate risk. Absolute risks were estimated, and age-adjusted AUC model performances (aAUCs) were compared across the 10-year period. RESULTS The aAUCs of the image-based risk model ranged from 0.74 (95% CI, 0.70 to 0.78) to 0.65 (95% CI, 0.63 to 0.66) for breast cancers developed 1-10 years after study entry; the corresponding Tyrer-Cuzick aAUCs were 0.62 (95% CI, 0.56 to 0.67) to 0.60 (95% CI, 0.58 to 0.61). For symptomatic cancers, the aAUCs for the image-based model were ≥0.75 during the first 3 years. Women with high and low mammographic density showed similar aAUCs. Throughout the 10-year follow-up, 20% of all women with breast cancers were deemed high-risk at study entry by the image-based risk model compared with 7.1% using the lifestyle familial-based model ( P < .01). CONCLUSION The image-based risk model outperformed the Tyrer-Cuzick v8 model for both short-term and long-term risk assessment and could be used to identify women who may benefit from supplemental screening and risk reduction strategies.
Approach to the Patient With a Suboptimal Statin Response: Causes and Algorithm for Clinical Management
Authors: Sun Lufan , Wolska Anna , Amar Marcelo , Zubirán Rafael , Remaley Alan T 
Published: March 2023

Abstract

Context
Statins are the lipid-lowering therapy of choice for the prevention of atherosclerotic cardiovascular disease (ASCVD) but their effectiveness in lowering low-density lipoprotein cholesterol (LDL-C) can substantially differ between individuals. In this mini-review, we describe the different causes for a suboptimal statin response and an algorithm for the diagnosis and clinical management of these patients.

Evidence Acquisition
A PubMed search using the terms “statin resistance,” “statin sensitivity,” “statin pharmacokinetics,” “cardiovascular disease,” and “lipid-lowering therapies” was performed. Published papers in the past 10 years that were relevant to the topic were examined to provide content for this mini-review.

Evidence Synthesis
Suboptimal lowering of LDL-C by statins is a major problem in the clinical management of patients and limits the value of this therapeutic approach. There are multiple causes of statin hyporesponsiveness with compliance being the most common explanation. Other causes, such as analytical issues with LDL-C measurement and the presence of common lipid disorders (familial hypercholesterolemia, elevated lipoprotein[a] and secondary dyslipidemias) should be excluded before considering primary statin resistance from rare genetic variants in lipoprotein-related or drug-metabolism genes. A wide variety of nonstatin lipid-lowering drugs are now available and can be added to statins to achieve more effective LDL-C lowering.

Conclusions
The evaluation of statin hyporesponsiveness is a multistep process that can lead to the optimization of lipid-lowering therapy for the prevention of ASCVD. It may also lead to the identification of distinct types of dyslipidemias that require specific therapies and/or the genetic screening of family members.
The Changing Face of Multiple Endocrine Neoplasia 2A: From Symptom-Based to Preventative Medicine
Authors: Machens Andreas , Lorenz Kerstin , Brandenburg Tim , Führer-Sakel Dagmar , Weber Frank , Dralle Henning 
Published: March 2023

Abstract

Context
Early genetic association studies yielded too high risk estimates for multiple endocrine neoplasia (MEN2A), suggesting a need for extended surgery.

Objective
The objective was to delineate temporal changes in MEN2A presentation by birth cohort analyses.

Methods
Birth cohort analyses (10-year increments; ≤1950 to 2011-2020) of carriers of rearranged during transfection (RET) mutations who underwent surgery for MEN2A.

Results
Included in this study were 604 carriers (155 index, 445 nonindex, 4 additional patients), with 237 carriers harboring high-risk mutations, 165 carriers moderate–high risk mutations, and 202 carriers low–moderate risk mutations. With increasing recency of birth cohorts, there was a continual decline in index patients from 41-74% to 0% (P &lt; .001) and of medullary thyroid cancer (MTC) from 96-100% to 0-33% (P &lt; .001). Node metastases diminished from 62-70% to 0% (P ≤ .001; high and low–moderate risk mutations), whereas biochemical cure after thyroidectomy surged from 17-33% to 100% (P ≤ .019; high and low–moderate mutations). Surgical interventions for MEN2A-related tumors were performed increasingly earlier, causing median carrier age to fall: from 51-63 to 3-5 years at thyroidectomy (P &lt; .001); from 46-51 to 24-25 years at first adrenalectomy (P ≤ .013; high and moderate–high risk mutations); and from 43.5-66 to 16.5-32 years at parathyroidectomy. MTC diameters were more effectively decreased from 14-32 to 1-4 mm (P ≤ 002) than pheochromocytoma diameters (nonsignificant).

Conclusion
These insights into MEN2A presentation, adjusted by birth year, illustrate the shift from reactive to preventative medicine, enabling less extensive risk-reducing surgery.
Altered Emotion Perception Linked to Structural Brain Differences in Youth With Congenital Adrenal Hyperplasia
Authors: Omary Adam , Khalifeh Noor , Cotter Devyn L , Kim Mimi S , Choudhury Farzana , Ahmadi Hedyeh , Geffner Mitchell E , Herting Megan M 
Published: March 2023

Abstract

Context
Congenital adrenal hyperplasia (CAH) is a genetic disorder that results in hormonal imbalances and decreased brain volumes in regions important for emotional processing.

Objective
To examine whether emotion perception differs between youth with CAH and control youth, and if these differences relate to brain volumes.

Methods
In this cross-sectional study of 27 youths with CAH (mean age = 12.63 years, 16 female) and 35 age- and sex-matched controls (mean age = 13.03 years, 20 female), each participant rated picture stimuli and completed a 3T structural brain scan. Valence and arousal ratings and reaction times of 61 affective images were assessed. Gray matter volumes were measured by MRI.

Results
Youth with CAH had lower valence ratings for negative (P = .007) and neutral (P = .019) images. Controls showed differences in reaction times and arousal ratings across stimuli conditions, but youth with CAH did not. Brain volumes of the right amygdala (P = .025) and left hippocampus (P = .002) were associated with valence ratings. Left rostral middle frontal (P &lt; .001) and right medial orbitofrontal cortex (P = .002) volumes were negatively related to valence scores only in youth with CAH, whereas left medial orbitofrontal cortex (P &lt; .001) volumes were associated with valence scores positively in youth with CAH and negatively in controls.

Conclusion
Findings suggest that youth with CAH perceive emotive stimuli as more unpleasant. Decreased brain volumes in the amygdala, hippocampus, and prefrontal cortex are associated with these measures of altered emotion perception in youth with CAH.