bluuebunny/crossref_metadata_2025_split huggingface dataset:
Life‐stage‐dependent effects of multiple flood‐associated stressors on a coastal foundational species
Authors: Pruett Jessica L. , Pandelides Ann Fairly , Keylon Jaycie , Willett Kristine L. , Showalter Otts Stephanie , Gochfeld Deborah J. 
Published: December 2022

AbstractGlobal changes in precipitation patterns have increased the frequency and duration of flooding events. Freshwater inflows into estuaries reduce salinity levels and increase nutrient inputs, which can lead to eutrophication and impaired water quality. Oysters are important ecosystem engineers in coastal environments that are vulnerable to co‐occurring environmental stressors associated with freshwater flooding events. Successful recruitment is necessary to maintain adult oyster populations, but early life stage responses to multiple stressors are not well understood. Flood‐associated stressor conditions were observed near oyster habitats at multiple locations across the northern Gulf of Mexico during peak recruitment months in the spring and summer of 2021. In the laboratory, we examined the interactive effects of acidification, hypoxia, and low salinity on larval and juvenile life stages of the eastern oyster (Crassostrea virginica) to better understand the impact of flooding events on oyster development and survival. Salinity stress in isolation reduced larval growth and settlement, and decreased survival and growth at the juvenile stage. Hypoxia was more stressful to oyster larvae than to juveniles, whereas low pH had negative effects on juvenile growth. There were no synergistic effects of multiple flood‐associated stressors on early oyster life stages and effects were either additive or predicted by the salinity stress response. The negative impacts of flooding disturbances on recruitment processes in benthic populations need to be considered in restoration planning and flood control mitigation strategies as the frequency and intensity of extreme freshwater events continue to rise worldwide.
A simple metric for predicting the timing of river phytoplankton blooms
Authors: Bruns Nicholas E. , Heffernan James B. , Ross Matthew R. V. , Doyle Martin 
Published: December 2022

AbstractIn rivers, phytoplankton populations are continuously exported by unidirectional, advective flow. Both transport and growth conditions determine periods of excess phytoplankton growth, or blooms, in a given reach. Phytoplankton abundance, however, has mainly been compared to the state of either growth or transport conditions alone rather than in tandem. Previous studies have not yielded consistent driver–response relationships, limiting our ability to predict the timing of riverine phytoplankton blooms based on environmental factors. Here, we derive a simple joint metric that combines the state of growth and transport conditions, specifically the ratio of temperature and discharge (). We then compare the metric to biomass abundance data (daily, sensor‐based chlorophyll a [chl a] data) from a mid‐sized Great Plains river (the Kansas). was an excellent predictor of low to high biomass, outperforming either variable alone. However, it could not differentiate between very high biomass values, values well above the biomass threshold designating bloom conditions. Our findings of reduced performance at the highest values of T/Q indicated that could predict the occurrence (timing) but not magnitude of phytoplankton blooms, and we used T/Q to correctly predict 71% of days when bloom conditions occurred. Analyzing chl a abundance with also revealed likely switching from transport and temperature to nutrient control. offers a simple tool for (1) predicting the timing of river phytoplankton blooms, (2) forecasting how river ecosystems will respond to surrounding environmental changes, and (3) determining which environmental factors shape phytoplankton blooms at specific locations along a river.
A Fujiwara‐Moritani‐Type Alkenylation Using a Traceless Directing Group Strategy: A Rare Example of C−C Bond Formation towards the C2‐Carbon of Terminal Alkenes
Authors: Pourkaveh Raheleh , Podewitz Maren , Schnürch Michael 
Published: December 2022

AbstractHerein we report, a rhodium‐catalyzed Fujiwara‐Moritani‐type reaction of unactivated terminal alkenes and benzoic acid derivatives bearing electron donating residues under mild conditions. The acid functionality acts as a traceless directing group delivering products alkenylated in meta‐position to the electron donating substituent in contrast to the usually obtained ortho‐ and para‐substitution in Friedel‐Crafts‐type reactions. Remarkably, the new C−C bond is formed to the C2 of the terminal olefin, in contrast to similar reported transformations. Initially formed mixtures of exo‐ and endo‐double bond isomers can be efficiently isomerized to the more stable endo‐products.
Forecasting swap rate volatility with information from swaptions
Authors: Liu Xiaoxi , Xie Jinming 
Published: December 2022

AbstractWe examine the predictability of the model‐free implied volatility from swaptions on future realized volatility of the underlying swap rates. The model‐free implied volatility demonstrates significant predictability on future realized volatility of swap rates along a wide cross‐section of tenors. The predictive power of the model‐free implied volatility is superior to the predictability of lagged realized volatility and generalized autoregressive conditional heteroskedasticity‐type conditional volatility. The superior predictive power of the model‐free implied volatility also holds out‐of sample, in different market states and with longer forecasting horizons.
Plant genome editing: CRISPR, base editing, prime editing, and beyond
Authors: Xie Yujie , Haq Syed Inzimam Ul , Jiang Xingyu , Zheng Dianfeng , Feng Naijie , Wang Wenying , He Jin‐Sheng , Qiu Quan‐Sheng 
Published: December 2022

AbstractThe clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR‐associated protein 9 (Cas9) system is a fast‐growing, genome editing technology that has wide applications in identifying gene functions as well as improving agricultural production and crop breeding. Here, we summarized recent advances in the development and applications of genome editing technologies in plants. We briefly described CRISPR/Cas9 technology and examined the base and prime editing techniques that have been developed from CRISPR technology. Some new prime editing‐derived techniques were assessed.
Effect of magnesium supplementation in improving hyperandrogenism, hirsutism, and sleep quality in women with polycystic ovary syndrome: A randomized, placebo‐controlled clinical trial
Authors: Gholizadeh‐Moghaddam Mahsa , Ghasemi‐Tehrani Hatav , Askari Gholamreza , Jaripur Mahsima , Clark Cain C. T. , Rouhani Mohammad Hossein 
Published: December 2022

AbstractBackground and AimsPolycystic ovary syndrome (PCOS) is one of the common endocrine disorders in women, which causes numerous symptoms in women. The relationship of many micronutrients with this syndrome has been investigated. This study was conducted to examine the effects of magnesium supplementation on hyperandrogenism, hirsutism, and sleep quality in women with PCOS.MethodsIn this parallel randomized clinical trial, 64 women with PCOS were randomly assigned to the magnesium group (n = 32) or placebo group (n = 32) for 10 weeks. Patients in the magnesium group received one 250 mg magnesium oxide tablet, per day. Hyperandrogenism, hirsutism, and sleep quality were measured at the beginning and end of the study. This randomized clinical trial was registered at https://www.IRCT.ir (IRCT20130903014551N8).ResultsMagnesium supplementation had no significant effect on hyperandrogenism (p = 0.51 for dehydroepiandrosterone sulfates, p = 0.27 for testosterone), hirsutism (p = 0.23), and sleep quality (p = 0.85) compared with placebo.ConclusionsThe present study showed that a single dose of magnesium supplementation elicited no beneficial effects on the mentioned symptoms in polycystic women. It is possible that the positive effects of magnesium observed in the former studies were due to the synergistic effects of other vitamins or minerals. More studies are needed in this area.
Demographic efficiency drivers in the Chinese energy production chain: A hybrid neural multi‐activity network data envelopment analysis
Authors: Zhao Yue , Antunes Jorge , Tan Yong , Wanke Peter 
Published: December 2022

AbstractFor meeting the external requirements of the Paris Agreement and reducing energy consumption per gross domestic product, China needs to improve its energy efficiency. Although the existing studies have attempted to investigate energy efficiency from different perspectives, little effort has yet been made to consider the collaboration among different stages in the production chain to produce energy outputs. In addition, various studies have also examined the determinants of energy efficiency, however, they mainly focused on technology and economic factors, no study has yet proposed and considered the influence of geographical factors on energy efficiency. In this article, we fill in the gap and make theoretical and empirical contributions to the literature. In this study, a two‐stage analysis method is used to analyse energy efficiency and the influencing factors in China between 2009 and 2021. More specifically, from the theoretical/methodological perspective, a multi‐activity network data envelopment analysis model is used to measure energy efficiency of different processes in the energy production chain. From the empirical perspective, we attempt to investigate the influence of geographical factors on energy efficiency through a neural network analysis. Meanwhile, the comparisons among different provinces are made. The result shows that the overall energy efficiency is low in China, and China relies more on the traditional energy industry than the clean energy industry. The efficiency level experiences a level of volatility over the examined period. Finally, we find that raw fuel pre‐process and industry have a significant and positive impact on energy efficiency in China.
Beyond access to adequate WASH facilities: Menstrual hygiene practices of high school adolescent girls
Authors: Usman Adam Muwaffaq , Kpeebi Yetimoni , Usman Adam Bahija , Adams Aisha , Sahabi Sakina 
Published: December 2022

AbstractIntroductionMenstrual hygiene has been a major problem facing Adolescent Girls at all levels of education in developing countries. Yet, it has not received the needed attention.MethodologyThis study uses univariate analysis to investigate the menstrual hygiene practices of adolescent girls in Ghana, with the adoption of the Menstrual Practices Questionnaire (MPQ). A sample of 167 randomly selected adolescent girls (mean age of 14.5) from five high schools in Ghana were surveyed one time. The survey was administered in a community where access to adequate WASH facilities is sorely lacking.ResultsDespite inadequate access to WASH facilities, majority of the girls' menstrual practices are hygienic. However, for a significant proportion of the girls, changing of menstrual materials (47.5%), washing of genitals (70.3%) and hands (70.9%), disposal of menstrual wastes (27.7%), sterilization of menstrual materials (31.4%), and management of cramps (64.5%) were found to be inadequate, poor, unhygienic, and unhealthy. Bridging this gap requires community‐wide sensitization and adequate provision of WASH facilities.ConclusionInadequate access to WASH facilities may not be as huge a hindrance to observing good menstrual hygiene among adolescent girls as reported in conventional literature. Sociocultural factors such as religion and culture might explain this phenomenon. The study therefore recommends further studies to explore factors influencing this phenomenon which would hopefully provide an idiosyncratic genesis that might go a long way in shaping the otherwise “biased” perspectives with which religion and culture are viewed in the menstrual hygiene discourse.
Biopharmaceutical and nanotoxicological aspects of cyclodextrins for non‐invasive topical treatments: A critical review
Authors: Riccio Bruno Vincenzo Fiod , Meneguin Andréia Bagliotti , Baveloni Franciele Garcia , de Antoni Juliana Aenishanslin , Robusti Leda Maria Gorla , Gremião Maria Palmira Daflon , Ferrari Priscileila Colerato , Chorilli Marlus 
Published: December 2022

AbstractCyclodextrins are nanometric cyclic oligosaccharides with amphiphilic characteristics that increase the stability of drugs in pharmaceutical forms and bioavailability, in addition to protecting them against oxidation and UV radiation. Some of their characteristics are low toxicity, biodegradability, and biocompatibility. They are divided into α‐, β‐, and γ‐cyclodextrins, each with its own particularities. They can undergo surface modifications to improve their performances. Furthermore, their drug inclusion complexes can be made by various methods, including lyophilization, spray drying, magnetic stirring, kneading, and others. Cyclodextrins can solve several problems in drug stability when incorporated into dosage forms (including tablets, gels, films, nanoparticles, and suppositories) and allow better topical biological effects of drugs at administration sites such as skin, eyeballs, and oral, nasal, vaginal, and rectal cavities. However, as they are nanostructured systems and some of them can cause mild toxicity depending on the application site, they must be evaluated for their nanotoxicology and nanosafety aspects. Moreover, there is evidence that they can cause severe ototoxicity, killing cells from the ear canal even when applied by other administration routes. Therefore, they should be avoided in otologic administration and should have their permeation/penetration profiles and the in vivo hearing system integrity evaluated to certify that they will be safe and will not cause hearing loss.
A first insight into haemocytes of Pinctada imbricata radiata: A morpho‐functional characterization
Authors: Pagano Maria , Fabrello Jacopo , Multisanti Cristiana Roberta , Zicarelli Giorgia , Ciscato Maria , Boldrin Francesco , Giacobbe Salvatore , Matozzo Valerio , Faggio Caterina 
Published: December 2022

AbstractThe pearl oyster Pinctada imbricata radiata (Leach, 1814), from the Pacific Ocean, was one of the first species to reach via Suez the Mediterranean, colonizing the eastern basin and recently spreading to the western. The species showed to be able to adapt to a wide range of climatic, hydrological, and ecological conditions. Since 2000 it reached the Strait of Messina, where is now infesting the transitional waters of the oriented natural reserve “Laguna di Capo Peloro.” Due to such resistance and adaptation ability, various assays were performed. Haemocyte morpho‐functional aspects were evaluated in haemolymph samples fixed with 1% and 2% glutaraldehyde for optical and electron microscopy (TEM). The following assays were carried out: cell characterization using several dyes, detection of intra‐ and extracellular lipids, the capability of phagocytosis using the yeast Saccharomyces cerevisiae and to produce superoxide anion (O2−). Detection of several enzymes, such as acid and alkaline phosphatase, arylsulfatase, chloro‐acetylesterase and β‐glucuronidase was also assessed. Cell count was demonstrated to be abundant with a mean of 8.263 × 106 mm2 ± 0.935 × 106 (SD). Two main cell populations were noticed: granulocytes and hyalocytes, both competent for phagocytosis, to produce O2−, and characterized by lipids. Based on the granule analysis, enzymatic activity was also demonstrated. The observations under TEM confirmed all the results obtained. This study supports the hypothesis that P. imbricata radiata can be usefully employed as a model organism in environmental biomonitoring. Moreover, since the species represent potential threats to native species and ecosystems, further insights into its biological adaptations in invaded ecosystems are recommended.
Human glial müller and umbilical vein endothelial cell coculture as an in vitro model to investigate retinal oxidative damage. A morphological and molecular assessment
Authors: Astolfi Gloria , Ciavarella Carmen , Valente Sabrina , Coslovi Chiara , Iannetta Danilo , Fontana Luigi , Pasquinelli Gianandrea , Versura Piera 
Published: December 2022

AbstractThe aim of this study was to optimize a coculture in vitro model established between the human Müller glial cells and human umbilical vein endothelial cells, mimicking the inner blood‐retinal barrier, and to explore its resistance to damage induced by oxidative stress. A spontaneously immortalized human Müller cell line MIO‐M1 and human umbilical vein endothelial cells (HUVEC) were plated together at a density ratio 1:1 and maintained up to the 8th passage (p8). The MIO‐M1/HUVECs p1 through p8 were treated with increasing concentrations (range 200–800 μM) of H2O2 to evaluate oxidative stress induced damage and comparing data with single cell cultures. The following features were assayed p1 through p8: doubling time maintenance, cell viability using MTS assay, ultrastructure of cell–cell contacts, immunofluorescence for Vimentin and GFAP, molecular biology (q‐PCR) for GFAP and CD31 mRNA. MIO‐M1/HUVECs cocultures maintained distinct cell cytotype up to p8 as shown by flow cytometry analysis, without evidence of cross activation, displaying cell–cell tight junctions mimicking those found in human retina, only acquiring a slight resistance to oxidative stress induction over the passages. This MIO‐M1/HUVECs coculture represents a simple, reproducible and affordable model for in vitro studies on oxidative stress‐induced retinal damages.
A novel, ultrafast, ultrasensitive diagnosis platform for the detection of SARS‐COV‐2 using restriction endonuclease‐mediated reverse transcription multiple cross displacement amplification
Authors: Huang Junfei , Yang Xinggui , Ren Lijuan , Jiang Weijia , Huang Yan , Liu Ying , Liu Chunting , Chen Xu , Li Shijun 
Published: December 2022

AbstractCoronavirus disease 2019 (COVID‐19) is a highly infectious disease caused by severe acute respiratory syndrome coronavirus 2 (SARS‐COV‐2). Though many methods have been used for detecting SARS‐COV‐2, development of an ultrafast and highly sensitive detection strategy to screen and/or diagnose suspected cases in the population, especially early‐stage patients with low viral load, is significant for the prevention and treatment of COVID‐19. In this study, a novel restriction endonuclease‐mediated reverse transcription multiple cross displacement amplification (MCDA) combined with real‐time fluorescence analysis (rRT‐MCDA) was successfully established and performed to diagnose COVID‐19 infection (COVID‐19 rRT‐MCDA). Two sets of specific SARS‐COV‐2 rRT‐MCDA primers targeting opening reading frame 1a/b (ORF1ab) and nucleoprotein (NP) genes were designed and modified according to the reaction mechanism. The SARS‐COV‐2 rRT‐MCDA test was optimized and evaluated using various pathogens and clinical samples. The optimal reaction condition of SARS‐COV‐2 rRT‐MCDA assay was 65°C for 36 min. The SARS‐COV‐2 rRT‐MCDA limit of detection (LoD) was 6.8 copies per reaction. Meanwhile, the specificity of SARS‐COV‐2 rRT‐MCDA assay was 100%, and there was no cross‐reaction with nucleic acids of other pathogens. In addition, the whole detection process of SARS‐COV‐2 rRT‐MCDA, containing the RNA template processing (15 min) and real‐time amplification (36 min), can be accomplished within 1 h. The SARS‐COV‐2 rRT‐MCDA test established in the current report is a novel, ultrafast, ultrasensitive, and highly specific detection method, which can be performed as a valuable screening and/or diagnostic tool for COVID‐19 in clinical application.
Improved mapping and understanding of land system states for achieving land degradation neutrality using remote sensing surface endmembers—temperature space: A case‐study in Minqin, China
Authors: Jiao Xin , Lin Xin , Duan Wenkai , Sun Qiangqiang , Chen Yong , Wen Na , Sun Minxuan , Zhang Ping , Zhang Yongxiang , Lun Fei , Sun Danfeng 
Published: December 2022

AbstractThe identification and understanding of land system states are critical for achieving land degradation neutrality (LDN). However, present conventional approaches can hardly generate a state space with both complex linear and abrupt shift relationships. Therefore, with the remote sensing surface endmembers—temperature space, this study developed a framework to identify, map and understand land system states and their associated trajectories based on the alternative state theory. Combined with three variables of land cover and landscape, our proposed vegetation productivity index (VPI) can highly improve the mapping accuracy of land system states in Minqin, without considering the variable of soil type. We built the three‐dimensional state catastrophe space with variables of soil organic matter (SOM), VPI, and cover function index (LCI). Thanks to the ball‐and‐cup model, two alternative stable states and five unstable states were identified at the landscape level in Minqin; then, we further explored the desired threshold of VPI and LCI were 13.11 and 0.39, while their undesired threshold were 5.15 and 0.76, respectively. The state space can reveal the linear or non‐linear relationship between VPI/LCI and SOM; besides, it can also present the hysteresis of land system state. Moreover, our established model in 2015 proved to be stable and thus can be used to accurately estimate the states in 2018. Therefore, our proposed framework can be an effective way of mapping and understanding present and future land system states, which can provide useful information on proactive conservation and restorative interventions for LDN management.
How perceived life control shapes sustainable consumption: The role of outcome efficacy
Authors: Zhang Jin , Sheng Shibin , Xu Xiaobing 
Published: December 2022

AbstractSustainable consumption helps solve today's critical environmental problems, but such behavior tends to be altruistic, mainly benefiting the environment and other people. Thus, understanding precursors that drive a consumer to engage in sustainable consumption is important for scholars and green product marketers. This study examines how perceived life control—a factor not directly related to environmental decisions—influences sustainable purchase. Data from two waves of the World Value Survey indicate that perceived life control is positively associated with pro‐environmental attitudes (which is a precondition for sustainable purchases). Five subsequent studies consistently show that perceived life control increases purchase intention for green products, and this happens because consumers with greater life control believe their purchases will be more effective in solving environmental problems (i.e., outcome efficacy). We further reveal that framing the benefits of a green product as temporally present (vs. future) attenuates the observed effect. These findings suggest that the practice of sustainable consumption may be explained by perceived life control in individuals and provide insights for environmental communication regarding the alignment between sustainable product appeals and individual differences.