bluuebunny/crossref_metadata_2025_split huggingface dataset:
Surface plasmon resonance based‐optical biosensor: Emerging diagnostic tool for early detection of diseases
Authors: Singh Abhinay Kumar , Anwar Masroor , Pradhan Rashmita , Ashar Mohd Suhail , Rai Nitish , Dey Sharmistha 
Published: March 2023

AbstractThe development of diagnostic tools remains at the center of the health care system. In recent times optical biosensors have been widely applied in the scientific community, especially for monitoring protein–protein or nucleic acid hybridization interactions. Optical biosensors‐derived surface plasmon resonance (SPR) technology has appeared as a revolutionary technology at the current times. This review focuses on the research work in molecular biomarker evaluation using the technique based on SPR for translational clinical diagnosis. The review has covered both communicable and noncommunicable diseases by using different bio‐fluids of the patient's sample for diagnosis of the diseases. An increasing number of SPR approaches have been developed in healthcare research and fundamental biological studies. The utility of SPR in the area of biosensing basically lies in its noninvasive diagnostic and prognostic feature due to its label‐free high sensitivity and specificity properties. This makes SPR an invaluable tool with precise application in the recognition of different stages of the disease.
Fluorescence attenuated by a thick scattering medium: Theory, simulations and experiments
Authors: Weber Yitzchak , Duadi Hamootal , Rudraiah Pavitra Sokke , Yariv Inbar , Yahav Gilad , Fixler Dror , Ankri Rinat 
Published: March 2023

AbstractFluorescence‐based imaging has an enormous impact on our understanding of biological systems. However, in vivo fluorescence imaging is greatly influenced by tissue scattering. A better understanding of this dependence can improve the potential of noninvasive in vivo fluorescence imaging. In this article, we present a diffusion model, based on an existing master–slave model, of isotropic point sources imbedded in a scattering slab, representing fluorophores within a tissue. The model was compared with Monte Carlo simulations and measurements of a fluorescent slide measured through tissue‐like phantoms with different reduced scattering coefficients (0.5–2.5 mm−1) and thicknesses (0.5–5 mm). Results show a good correlation between our suggested theory, simulations and experiments; while the fluorescence intensity decays as the slab's scattering and thickness increase, the decay rate decreases as the reduced scattering coefficient increases in a counterintuitive manner, suggesting fewer fluorescence artifacts from deep within the tissue in highly scattering media.
Secreted phosphoprotein 24 kD (Spp24) inhibits the growth of human osteosarcoma through the BMP‐2/Smad signaling pathway
Authors: Chen Hongfang , Li Chenshuang , Zhou Tangjun , Li Xunlin , Duarte Maria Eugenia L. , Daubs Michael D. , Buser Zorica , Brochmann Elsa J. , Wang Jeffrey C. , Murray Samuel S. , Jiao Li , Tian Haijun 
Published: March 2023

AbstractAutocrine stimulation of tumor cells is an important mechanism for the growth of skeletal tumors. In tumors that are sensitive, growth factor inhibitors can dramatically reduce tumor growth. In this study, our aim was to investigate the effects of Secreted phosphoprotein 24 kD (Spp24) on the growth of osteosarcoma (OS) cells in the presence and absence of exogenous BMP‐2 both in vitro and in vivo. Our study demonstrated that Spp24 inhibited proliferation and promoted apoptosis of OS cells as confirmed by 3‐(4,5‐dimethylthiazolyl‐2)‐2,5‐diphenyltetrazolium bromide assay and immunohistochemical staining. We found that BMP‐2 increased the mobility and invasiveness of tumor cells in vitro whereas Spp24 inhibited both of these processes alone and in the presence of exogenous BMP‐2. Phosphorylation of Smad1/5/8 and Smad8 gene expression was enhanced by treatment with BMP‐2 but inhibited by treatment with Spp24. Subcutaneous and intratibial tumor models in nude mice demonstrated that BMP‐2 promoted OS growth in vivo, while Spp24 significantly inhibited tumor growth. We conclude that the BMP‐2/Smad signaling pathway is involved in the pathogenesis of OS growth and that Spp24 inhibits the growth of human OS induced by BMP‐2 both in vitro and in vivo. Interruption of Smad signaling and increased apoptosis appear to be the primary mechanisms involved. These results confirm the potential of Spp24 as a therapeutic agent for the treatment of OS and other skeletal tumors.
Prospects for the application of traditional Chinese medicine network pharmacology in food science research
Authors: Su Yuanyuan , Bai Qiong , Tao Hongxun , Xu Bin 
Published: March 2023

AbstractThere has always been a particular difficulty with in‐depth research on the mechanisms of food nutrition and bioactivity. The main function of food is to meet the nutritional needs of the human body, rather than to exert a therapeutic effect. Its relatively modest biological activity makes it difficult to study from the perspective of general pharmacological models. With the popularity of functional foods and the concept of dietary therapy, and the development of information and multi‐omics technology in food research, research into these mechanisms is moving towards a more microscopic future. Network pharmacology has accumulated nearly 20 years of research experience in traditional Chinese medicine (TCM), and there has been no shortage of work from this perspective on the medicinal functions of food. Given the similarity between the concept of ‘multi‐component‐multi‐target’ properties of food and TCM, we think that network pharmacology is applicable to the study of the complex mechanisms of food. Here we review the development of network pharmacology, summarize its application to ‘medicine and food homology’, and propose a methodology based on food characteristics for the first time, demonstrating its feasibility for food research. © 2023 Society of Chemical Industry.
Analysis of Complications in (Crico‐) Tracheal Resection Anastomosis in Adults: A Multicenter Study
Authors: Fiz Ivana , Filauro Marta , Sampieri Claudio , Ioppi Alessandro , Vallin Alberto , Fiz Francesco , Koelmel Jan Costantin , Lancini Davide , Piazza Cesare , Sittel Christian , Peretti Giorgio 
Published: March 2023

ObjectivesThe gold standard treatments for advanced laryngotracheal stenosis (LTS) are represented by partial crico‐tracheal (PCTRA) or tracheal resection and anastomosis (TRA). These procedures are potentially burdened by high postoperative complication rates. We investigated the impact of the most common stenosis and patient‐related characteristics on the onset of complications in a multicentric cohort.MethodsWe retrospectively analyzed patients who underwent PCTRA or TRA for LTS of different etiologies in three referral centers. We tested the effectiveness of these procedures, the impact of complications on the outcomes, and identified factors causing postoperative complications.ResultsA total of 267 patients were included in the study (130 females; mean age, 51.46 ± 17.64 years). The overall decannulation rate was 96.4%. Altogether, 102 (38.2%) patients presented at least one complication, whereas 12 (4.5%) had two or more. The only independent predictor of post‐surgical complications was the presence of systemic comorbidities (p = 0.043). Patients experiencing complications needed additional surgery more frequently (70.1% vs. 29.9%, p < 0.001), and had a longer duration of hospitalization (20 ± 10.9 vs. 11.3 ± 4.1 days, p < 0.001). Six of 102 (5.9%) patients with complications had restenosis, although this event did not occur among patients without complications.ConclusionPCTRA and TRA have an excellent success rate even when performed for high‐grade LTS. However, a significant percentage of patients may experience complications associated with a longer duration of hospitalization or the need for additional surgeries. The presence of medical comorbidities was independently related to an increased risk of complications.Level of evidence4 Laryngoscope, 133:2910–2919, 2023
Online Searches Related to Total Laryngectomy
Authors: Wu Michael P , Miller Lauren E , Meyer Charles D , Feng Allen L , Richmon Jeremy D 
Published: March 2023

ObjectiveTo identify the most frequently asked questions regarding “laryngectomy” through an assessment of online search data.MethodsGoogle Search data based on the search term “laryngectomy” were analyzed using Google Trends and Search Response. The most common People Also Ask (PAA) questions were identified and classified by the concept. Each website linked to its respective PAA question was rated for understandability, ease of reading, and reading grade level.ResultsSearch popularity for the term “laryngectomy” remained stable between 2017 and 2022. The most popular PAA themes were post‐laryngectomy speech, laryngectomy comparison to tracheostomy, stoma and stoma care, survival/recurrence, and post‐laryngectomy eating. Of the 32 websites linked to the top 50 PAA's, eleven (34%) were at or below an 8th grade reading level.ConclusionPost‐laryngectomy speech, eating, survival, the stoma, and the difference between laryngectomy and tracheostomy are the most common topics searched online in relation to “laryngectomy.” These are important areas for both patient and healthcare provider education.Level of EvidenceN/A Laryngoscope, 133:2971–2976, 2023
Assessment of adverse events for a home‐use intense pulsed light hair removal device using postmarketing surveillance
Authors: Hattersley Anne M. , Kiernan Michael , Goldberg David , Dierickx Christine , Sliney David H. , Haedersdal Merete , Nash J. Frank 
Published: March 2023

AbstractBackground and objectivesHome‐use intense pulsed light (IPL) hair removal devices are convenient for consumers. Consumer safety associated with home‐use IPL devices, however, remains a subject of interest. In this descriptive analysis, we assessed the most commonly reported adverse events (AEs) for a home‐use IPL device from postmarketing surveillance and qualitatively compared these with AEs from clinical studies and medical device reports of home‐use IPL treatments.Materials and methodsFor this analysis of voluntary reports, we queried a distributor′s postmarketing database for IPL devices for the period beginning January 1, 2016, to December 31, 2021. All sources of comments, for example, phone, e‐mail, company‐sponsored web sites, were included in the analysis. AE data were coded according to the Medical Dictionary for Regulatory Activities (MedDRA) terminology. Also, we conducted a PubMed search to identify AE profiles from existing literature on home‐use IPL devices and we searched the Manufacturer and User Facility Device Experience (MAUDE) database for reports on home‐use IPL devices. These results were qualitatively compared to the data in the postmarketing surveillance database.ResultsA total of 1692 cases involving IPL were identified from voluntary reports of AEs between 2016 and 2021. The shipment‐adjusted reporting rate for AE cases (number of AE cases/100,000 shipped IPL devices) was 67/100,000 during this 6‐year period. The most commonly reported AEs were pain of skin 27.8% (470/1692), “thermal burn” 18.7% (316/1692), and erythema 16.0% (271/1692). Among the top 25 AEs reported, no unexpected health events were observed. The reported AEs were qualitatively similar to the pattern seen in clinical studies and the MAUDE database associated with such home‐use IPL treatments.ConclusionThis is the first such report documenting AEs for home‐use IPL hair removal from a postmarketing surveillance program. These data are supportive of the safety of such home‐use low‐fluence IPL technology.
Combining Imaging Sonar Counting and Underwater Camera Species Apportioning to Estimate the Number of Atlantic Salmon and Striped Bass in the Miramichi River, New Brunswick, Canada
Authors: Helminen J. , Linnansaari T. 
Published: March 2023

Abstract
A combined method incorporating an imaging sonar and underwater cameras was tested for assessing the size of adult Atlantic Salmon Salmo salar and Striped Bass Morone saxatilis populations in one of the main tributaries of the Miramichi River, New Brunswick, Canada. The number of fish recorded with the sonar in October 2019 was apportioned using the species ratio from the underwater camera data. The combined method estimated 358 Atlantic Salmon and 255 Striped Bass when the species ratio was applied every day and 274 Atlantic Salmon and 337 Striped Bass when the monthly species ratio was applied. The counts were compared to catches in a downstream index trap net using estimated values for trap-net catchability and for the proportion of fish ascending to the same tributary. Depending on the estimated values, the sonar–camera counts were between 40% and 190% of the estimated Atlantic Salmon numbers in the index net. For Striped Bass, the same estimated catchability and proportion values produced a lower agreement (sonar–camera count = 5–24% of the adjusted catch) because unlike Atlantic Salmon, Striped Bass do not deterministically migrate up the tributary in autumn. The fish were mostly detected overnight, and the trends in daily numbers of fish detected with the combined sonar–camera method were similar to the catches in the index net, with most Atlantic Salmon being detected mid-month and most Striped Bass being detected at the end of the month. The similarity of the fish counts with the adjusted trap-net catch and the new information about migration timing demonstrate that the sonar–underwater camera combination can provide tributary-specific and timely information on the Atlantic Salmon population in the Miramichi River.
‘Sometimes you need an eye‐opener’: A qualitative study on nursing assistants' experiences of developing communication skills through an educational intervention on person‐centred communication
Authors: Gustafsson Tanja , Maurin Söderholm Hanna , Sundler Annelie J. , Karlsson Pernilla , Lindberg Elisabeth 
Published: March 2023

AbstractAimTo explore nursing assistants' (NAs') experiences of developing communication skills while participating in an educational intervention on person‐centred communication.DesignA descriptive qualitative study was conducted.MethodsData were collected from interviews and written assignments before, during and after an educational intervention on person‐centred communication targeting NAs in home care services. The data were analysed using a phenomenological approach. A total of 25 NAs participated in the study.ResultsThe findings describe NAs' experiences concerning the communication skills needed for building relationships with older persons and handling emotionally challenging situations. The educational intervention increased their knowledge and awareness of the importance of communication skills and how such skills are developed and refined.
Deep learning system to predict the three‐dimensional contact status between the mandibular third molar and mandibular canal using panoramic radiographs
Authors: Fukuda Motoki , Kise Yoshitaka , Nitoh Munetaka , Ariji Yoshiko , Fujita Hiroshi , Katsumata Akitoshi , Ariji Eiichiro 
Published: March 2023

AbstractAimWe aim to evaluate the diagnostic performance of a deep learning (DL) system for determining the three‐dimensional contact status between the mandibular third molar and canal on panoramic radiography images.MethodsA total of 800 image patches consisting of 400 patches of low‐ and high‐risk groups, each verified by computed tomography (CT) or cone‐beam CT for dental use, were cropped from downloaded panoramic images and input into a DL system. Seven hundred of these patches (350 high‐risk and 350 low‐risk group patches) were randomly assigned to the training and validation datasets, and 100 (50 high‐risk and 50 low‐risk group patches) were assigned to the test datasets. Using data augmentation for the training datasets, the training process was carried out twice. Receiver operating characteristic (ROC) analysis was used to compare the performance of two kinds of observers (residents and radiologists) with the same test images. The interclass correlation coefficients (ICCs) were determined to evaluate the diagnostic consistency.ResultsThe area under the ROC curves (AUCs) of the DL model, residents, and radiologists were 0.85, 0.55, and 0.81, respectively. Significant differences were observed between the DL model and residents, and between the residents and radiologists. The ICCs of the DL model, residents, and radiologists were 0.69, 0.19, and 0.54, respectively.ConclusionsThe DL model has potential for use in diagnostic support in the evaluation of the three‐dimensional contact status between the mandibular third molar and canal on panoramic images.
Formation of nanoparticle aggregates and agglomerates in polymer nanocomposites and their distinct impacts on the mechanical properties
Authors: Sharifzadeh Esmail , Karami Mahshad , Ader Fiona 
Published: March 2023

AbstractIn this study, the different effects of nanoparticle aggregates and agglomerates on the mechanical properties of polymer nanocomposites are comprehensively investigated. To this end, a specific strategy, based on the equilibrium between the dispersion and cohesion energies in the mixing stage, is proposed using which the content and size of aggregates/agglomerates can be defined. The aggregated/agglomerated networks are considered to place in constrained volumes (CVs), having co‐continuous morphology. An equivalent box model (EBM), corresponding to the system, is used to predict the tensile modulus of the nanocomposite. Different test results of HDPE nanocomposite samples, containing 1–3 wt.% of surface‐modified silica nanoparticles, prepared by a semi‐industrial single screw extruder, are applied to validate the model. Moreover, other data from the literature are also used to further evaluate the accuracy and capability of the proposed analytical method.
Functional analysis of protein post‐translational modifications using genetic codon expansion
Authors: Peng Tao , Das Tandrila , Ding Ke , Hang Howard C. 
Published: March 2023

AbstractPost‐translational modifications (PTMs) of proteins not only exponentially increase the diversity of proteoforms, but also contribute to dynamically modulating the localization, stability, activity, and interaction of proteins. Understanding the biological consequences and functions of specific PTMs has been challenging for many reasons, including the dynamic nature of many PTMs and the technical limitations to access homogenously modified proteins. The genetic code expansion technology has emerged to provide unique approaches for studying PTMs. Through site‐specific incorporation of unnatural amino acids (UAAs) bearing PTMs or their mimics into proteins, genetic code expansion allows the generation of homogenous proteins with site‐specific modifications and atomic resolution both in vitro and in vivo. With this technology, various PTMs and mimics have been precisely introduced into proteins. In this review, we summarize the UAAs and approaches that have been recently developed to site‐specifically install PTMs and their mimics into proteins for functional studies of PTMs.
A facile one‐pot route to fabricate clothianidin‐loaded ZIF‐8 nanoparticles with biocompatibility and long‐term efficacy
Authors: Ma Yue , Zhao Kefei , Ding Yi , Wu Shuai , Liao Xun , Yin Xianhui , Li Zilu , Li Rongyu , Long Youhua , Du Fengpei 
Published: March 2023

AbstractBACKGROUNDNeonicotinoids are among the most essential chemical insecticides worldwide because of their high activity against many important pests and wide application. However, their application is limited by their toxicity to honeybees. Therefore, the development of a facile route to fabricate efficient and eco‐friendly pesticide formulations is of great significance.RESULTSIn this study, clothianidin‐loaded zeolitic imidazolate framework‐8 (CLO@ZIF‐8) nanoparticles were fabricated by a facile one‐pot route using zinc nitrate as a Zn2+ source and characterized by scanning electron microscopy, transmission electron microscopy, X‐ray diffraction, thermogravimetric analysis, energy‐dispersive spectrometry and Fourier transform infrared spectroscopy. Based on the pH response of ZIF‐8, a ‘burst release effect’ was observed for CLO@ZIF‐8 at pH 3 and 5 within 12 h, in contrast to the slow and sustainable release at pH 8. CLO@ZIF‐8 improved the retention ability of the pesticide liquid and remained 70% control efficacy on Nilaparvata lugens after water rinsed of sprayed CLO@ZIF‐8. The pH response of CLO@ZIF‐8 allowed it to maintain 43% control efficacy against N. lugens after 10 days of application, which was twice the efficacy of clothianidin solution (SCA). Moreover, CLO@ZIF‐8 reduced the acute toxicity to honeybees (Apis mellifera) by ≥120‐fold compared with SCA.CONCLUSIONThis study provides new insights into the application of ZIF‐8 to neonicotinoids and suggests the need for the development of a biocompatible and eco‐friendly pesticide formulation. © 2023 Society of Chemical Industry.
Novel 1‐Indanone derivatives containing oxime and oxime ether moieties as immune activator to resist plant virus
Authors: Lan Shichao , Zhang Wei , Gan Xiuhai 
Published: March 2023

AbstractBackgroundVegetable viruses are difficult to prevent and control in the field, causing massive economic losses of agricultural production in the world. A new natural product‐based antiviral agent would be an effective means to control viral diseases. As a class of natural products, 1‐indanones present various pharmacologically actives, while their application in agriculture remains to be found.ResultsA series of novel 1‐indanone derivatives were designed and synthesized and the antiviral activities were systematically evaluated. Bioassays showed that most compounds exhibited good protective activities against cucumber mosaic virus (CMV), tomato spotted wilt virus (TSWV), and pepper mild mottle virus (PMMoV). Notably, compound 27 exhibited the best protective effects against PMMoV with EC50 values of 140.5 mg L−1, superior to ninanmycin (245.6 mg L−1). Compound 27 induced immunity responses through multilayered regulation on mitogen‐activated protein kinase, plant hormone signal transduction and phenylpropanoid biosynthesis pathways.ConclusionThese 1‐indanone derivatives especially compound 27 can be considered as potential immune activators to resist plant virus. © 2023 Society of Chemical Industry.