bluuebunny/crossref_metadata_2025_split huggingface dataset:
An update to: Tracking TAU in neurons: How to transfect and track exogenous TAU in primary neurons
Authors: Buchholz Sarah , Bell-Simons Michael , Zempel Hans 
Published: January 2023

Abstract
Primary murine neurons have proved to be an essential tool for the general investigation of neuronal polarity, polarized TAU distribution, and TAU-based neuronal dysfunction in disease paradigms. However, mature primary neurons are notoriously difficult to transfect with non-viral approaches and are very sensitive to cytoskeletal manipulation and imaging. Furthermore, standard non-viral transfection techniques require the use of a supportive glia monolayer or high-density cultures, both of which interfere with microscopy. Here we provide a simple non-viral liposome-based transfection method that enables transfection of TAU in low levels comparable to endogenous TAU. This allows the investigation of e.g., distribution and trafficking of TAU, without affecting other cytoskeleton-based parameters such as microtubule density or microtubule-based transport. Using this protocol, we achieve a profound transfection efficiency, but avoid high overexpression rates. Importantly, this transfection method can be applied to neurons at different ages and is also suitable for very old cultures (up to 18 days in vitro). In addition, the protocol can be used in cultures without glial support and at suitable cell densities for microscopy-based single-cell analysis. In sum, this protocol has proven a reliable tool suitable for most microscopy-based approaches in our laboratory.
Understanding the Potential of Light Absorption in Dots-in-Host Semiconductors
Authors: Alexandre Miguel , Águas Hugo , Fortunato Elvira , Martins Rodrigo , Mendes Manuel J. 
Published: January 2023

Abstract
The outstanding physical properties of dots-in-host (QD@Host) hetero semiconductors demand detailed methods to fundamentally understand the best routes to optimize their potentialities for different applications. In this work, a 4-band k.p-based method was developed for rocksalt QDs that describes the complete optical properties of arbitrary QD@Host systems, trailblazing the way for the full opto-electronic analysis of quantum-structured solar cells. Starting with the determination of the QD bandgap, and validation against well-established literature results, the electron transition rate is then computed and analysed against the main system parameters. This is followed by a multi-parameter optimization, considering intermediate band solar cells as a promising application, where the best QD configuration was determined, together with the corresponding QD@Host absorption spectrum, in view of attaining the theoretical maximum efficiency (~ 50%) of this photovoltaic technology. The results show the creation of pronounced sub-bandgap absorption due to the electronic transitions from/to the quantum-confined states, which enables a much broader exploitation of the sunlight spectrum.
The Study of Absoluteness Overlooked by Special Relativity
Authors: Koh Hyoungseok 
Published: January 2023

Abstract
This study has reinterpreted the relativity of the laws of physics, an axiom of the special theory of relativity. Einstein interpreted that the relativity of the laws of physics is established due to the relativity of the inertial system. However, he did not consider that relativity was established despite the absoluteness of the inertial system being established. In this paper, we have assumed that relativity holds despite the absoluteness of the inertial system. This perspective is called observer relativity and is used distinguish it from the relativity of the inertial system. Relativity between the observers is expressed using the Lorentz and inverse transform since lights are used by the observers in both cases. To prove the absoluteness of the inertial system, a rigid ruler and light are used. If the observers measure the length with a rigid ruler and light, the reference system is consistent but the system of motion is different. This is defined as the absoluteness of the inertial system. To prove this absoluteness, several experiments were conducted to measure the electrostatic force between two electric charges fixed at the same distance. If the isotropy of space is satisfied, a stationary system can be defined. Conversely, it is defined as a constant velocity system. In the inertial system, the relativity between observers and absoluteness of the inertial system coexist.
High-depth whole-genome sequencing identifies structure variants, copy number variants and short tandem repeats associated with Parkinson’s disease
Authors: Chan Piu , Wang Chaodong , Liu Hankui , Li Xu-Ying , Ma Jinghong , Gu Zhuqin , Feng Xiuli , Xie Shu , Tang Beisha , Chen Shengdi , Wang Wei , Wang Jian , Zhang Jianguo 
Published: January 2023

Abstract

While numerous single nucleotide variants and small indels have been identified in Parkinson’s disease (PD), the genome-wide contribution of structural variants (SVs), copy number variants (CNVs) and short tandem repeats (STRs) remains poorly understood. Here we investigated the association between these variants and PD using the high-depth whole-genome sequencing data from 466 PD patients and 513 healthy elderlies. A total of 29,561 SVs, 32,153 CNVs and 174,905 STRs were detected. Overall, CNV deletions were significantly enriched in the end-proportion of autosomal chromosomes in PD. Genome-wide association study identified 11 novel signals (1 SV, 4 CNVs and 6 STRs) reaching genome-wide significance. Among these, the deletion nearby MUC19 and the 5-copy GGGAAA repeat in SLC2A13 reduced the penetrance of LRRK2 G2385R variant. Moreover, genes with these variants were specifically expressed in dopaminergic neurons and highly dosage-sensitive. These data provided novel insights into the genetic architecture of PD.
Unprecedented 2022 Yangtze River Valley heatwaves linked to the record-breaking Pakistan flood and triple-dip La Niña
Authors: Qiao Shaobo , Tang Shankai , Wang Bin , Liu Fei , Feng Taichen , Yang Jie , He Meng , Chen Dong , Cheng Jianbo , Feng Guolin , Dong Wenjie 
Published: January 2023

Abstract
In July-August 2022, Yangtze River valley (YRV) experienced unprecedented hot summer, with the number of heatwave days exceeding climatology by four standard deviations. The heatwaves and associated severe droughts affected about 38 million people and caused devastating economic losses of about five billion US dollars. Here we present convergent empirical and modelling evidence to show that the record-breaking Pakistan rainfall, along with the 2022 tripe-dip La Niña, produces anomalous high pressure over YRV, causing intense heatwaves. The La Niña-induced second-highest sea surface temperature gradient in the equatorial western Pacific suppresses western Pacific convection and extends the subtropical high westward. More importantly, the tremendous diabatic heating associated with the unprecedented Pakistan rainfall reinforces the downstream Rossby wave train, extending the upper-level South Asia High eastward and controlling the entire YRV. The overlay of the two high-pressure systems sustains sinking motion and increases solar radiation reaching the ground, causing recurrent heat waves.
Proportion of vitamin D deficiency in children/adolescents with type 1 diabetes: A systematic review and meta-analysis
Authors: Yang Xin , Chai Min , Lin Meng 
Published: January 2023

Abstract
Background: The impact of vitamin D on type 1 diabetes has been a controversial topic in public health. Furthermore, significant differences in the proportion of vitamin D have been noted. The purpose of this systematic review was to determine the overall proportion of vitamin D deficiency in children/adolescents with type 1 diabetes (T1D).
Methods: Based on six electronic databases (PubMed, Web of Science, Embase, Ovid Medline, ProQuest, and Cochrane Library), eligible studies since the databases’ inception up to April 2022 were searched. Reference lists were also manually searched to identify additional studies. Overall, studies with statistical information on vitamin D deficiency in children and adolescents with T1D were included, and a random effects model was applied for the meta-analysis. In addition, subgroup and sensitivity analyses were carried out to evaluate heterogeneity, and publication bias was evaluated by using Egger’s test.
Results: A total of 45 studies involving 6,995 participants were selected for the meta-analysis. The proportion of vitamin D deficiency in children/adolescents with T1D was found to be 45% (95% confidence interval [CI] 37-54%, I2 = 97.94%). Subgroup analysis further showed that the publication year, study design, classification of vitamin D, different seasons and geographical region could significantly contribute to variations in the reported proportion of vitamin D deficiency.
Conclusions: This systematic review analyzed the available literature and revealed that the proportion of vitamin D deficiency among T1D children/adolescents was 45%. In addition, the proportion remains higher, which has important implications for adapting health and social care systems.
A Large-Dynamic-Range Violet Phosphorus Heterostructure Optoelectronic Synapse for High-Complexity Neuromorphic Computing
Authors: Liu Xiaoxian , Wang Shuiyuan , Di Ziye , Wu Haoqi , Liu Chunsen , Zhou Peng 
Published: January 2023

Abstract
Neuromorphic computing can efficiently handle data-intensive tasks and address the redundant data interaction required by traditional von Neumann architectures. Synaptic devices are essential components for neuromorphic computation. For high computational accuracy, synaptic devices need to retain good conductance linearity, but this leads to a limited dynamic range (10 ~ 100) and weight states, which impedes their processing of high-complexity tasks and restricts further advances in accuracy. Two-dimensional materials, such as transition metal disulfides and phosphorene, hold promise for the construction of synaptic devices with large dynamic ranges due to their strong light-matter interactions, while the stability of phosphorene remains an issue. Here, for the first time, we use the most stable violet phosphorene for device applications. The combination of violet phosphorene and molybdenum disulfide demonstrates an optoelectronic synapse with a record dynamic range of over 106, benefiting from a significant threshold shift due to charge transfer and trapping in the heterostructure. Remarkable synaptic properties are demonstrated, including 128 distinguishable conductance states, electro-optical dependent plasticity, short-term paired-pulse facilitation, and long-term potentiation/depression. High-precision image classification with accuracies of 95.23% and 79.65% is achieved for MNIST and high-complexity Fashion-MNIST datasets, which is close to the ideal device (95.47%, 79.95%), indicating the potential of dynamic range and multi-states for optimizing accuracy. This work fills the device application gap of violet phosphorene and provides a strategy for building synaptic devices with large dynamic range to facilitate neuromorphic computing.
CD4highCD8low double-positive T cells with high differentiation mediate incomplete immune reconstitution in HIV-infected patients
Authors: Zhao Yu , Jia Jie , Zhang Yizhi , Mou Tangwei , Zhao Qi-Hui , Kong Deshenyue , Li Shao-You , Yang Ji-Qun , Mao Jun-Hong , Zhang Xiu-Ling , Su Bin , Kuang Yi-Qun 
Published: January 2023

Abstract
Antiretroviral therapy (ART) is the most generally used to treat human immunodeficiency virus (HIV)-infected patients. However, some patients experience incomplete immune reconstitution and fail to restore CD4+ T cell counts after treating with ART. Herein, we investigated the characters of CD4+CD8+ double-positive (DP) T cells at the transcriptomic level by analyzing single-cell RNA sequencing of peripheral blood mononuclear cells from HIV-infected immunological non-responders (INRs) and immunological responders (IRs). We identified eight DP T cell clusters and grouped into three populations CD4highCD8low, CD4lowCD8high, and CD4lowCD8low, respectively. Decreased proportion of DP T cells was detected in INRs, including CD4highCD8low DP T cells in the process of cellular differentiation, the latter demonstrated a reduced source of DP T cells in INRs. CD4highCD8low DP T cells in IRs and INRs as a heterogenous population, were distinct on the expression of GZMA/B/H, LAG3, NKG7 and GNLY, which related to the function of cell activation and cellular differentiation, cytotoxicity and programmed cell death. These data provide a comprehensive analysis of gene signatures of CD4highCD8low DP T cells associated with failed immune reconstitution after HIV infection, which could be useful in developing new cure strategies for HIV-infected patients.
Low-cost pathways to synthesize silica-smectite clay-based composites
Authors: Kepdieu Jean Marie , Djangang Chantale Njiomou , Njimou Jacques Romain , Maicaneanu Sanda Andrada , Mache Jacques Richard 
Published: January 2023

Abstract
The present study aimed at using rice husk as a natural silica precursor in the fabrication of silica-smectite composites. A local smectite clay was respectively mixed with 1) silica sludge from rice husk ashes after an acid treatment, 2) an aqueous sodium silicate solution from alkaline dissolution of silica sludge and 3) a nanosilica powder obtained after hydroxylation/polymerisation of a sodium silicate solution. Products from the three different synthesis pathways were investigated by XRD, infrared spectrometry, SEM/EDX and BET specific surface measurement. They show a heterogeneous morphology where the distribution of silica particles in the clay matrix change with each synthesis pathway. For silica sludge synthesis pathway, a predominated three-dimensional-like structure with a phyllosilicate matrix skeleton was obtained. For the pathway using a silicate solution, we obtained an amorphous compound having a limited intergranular cohesion and containing silicate agglomerates that are intercalated between clay sheets. It is different for the nanosilica reinforced pathway that led to a packed morphology with a regular distribution of silica phases in the clay matrix. In all the synthesized composites, we observe the occurrence of amorphous silica phases that favours both the existence of porosity and the presence of chemically active sites. Consequently, obtained products are potential multifunctional materials.
Pyruvate dehydrogenase complex integrates the metabolome and epigenome in memory T cell differentiation in vitro
Authors: Tarasenko Tatiana N. , Banerjee Payal , Gomez-Rodriguez Julio , Gildea Derek , Zhang Suiyuan , Wolfsberg Tyra , Jenkins Lisa M. , Program NISC Comparative Sequencing , McGuire Peter J. 
Published: January 2023

Abstract
Background
Modulation of metabolic flux through pyruvate dehydrogenase complex (PDC) plays an important role in T cell activation and differentiation. PDC sits at the transition between glycolysis and the tricarboxylic acid cycle and is a major producer of acetyl-CoA, marking it as a potential metabolic and epigenetic node.
Methods
To understand the role of pyruvate dehydrogenase complex in T cell differentiation, we generated mice deficient in T cell pyruvate dehydrogenase E1A (Pdha) subunit using a CD4-cre recombinase-based strategy. To control for the contribution of exogenous metabolites in vivo, we conducted our T cell functional studies in vitro. T cells were differentiated into memory and effector T cells using standardized protocols. Cells were analyzed using stable isotopic tracing studies, metabolomics, RNAseq, ATACseq, ChIPseq and histone proteomics.
Results
Herein, we show that genetic ablation of PDC activity in T cells (TPdh-/-) leads to marked perturbations in glycolysis, the tricarboxylic acid cycle, and OXPHOS. Due to depressed OXPHOS, TPdh-/- T cells became dependent upon substrate level phosphorylation via glycolysis. Due to the block of PDC activity, histone acetylation was reduced, as were most other types of post translational modifications. Transcriptional and functional profiling revealed abnormal CD8+ memory T cell differentiation in vitro.
Conclusions
Collectively, our data indicate that PDC integrates the metabolome and epigenome in memory T cell differentiation. Targeting this metabolic and epigenetic node can have widespread ramifications on cellular function.
SET-mediated epigenetic dysregulation of p53 impairs Trichloroethylene-induced DNA damage response
Authors: ren Xiaohu , Ruan Jiawen , Yang Sixia , Lan Xuerao , Wu Desheng , Huang Xinfeng , Zhang Hongyu , Huang Haiyan , Liu Jianjun 
Published: January 2023

Abstract
Trichloroethylene (TCE) is a major ground water pollutant which causes severe hepatic toxicity. Previously, we have identified histone chaperon, SET as a key mediator of TCE-induced liver cell apoptosis. DNA damage with p53 suppression and reduced tri-methylation at histone H3K79 (H3K79me3) were also found under the treatment of TCE. However, epigenetic mechanisms in TCE-associated DNA damage response still remain largely unknown. In this study, we evaluated TCE-induced DNA damage of hepatic cells in the absence of SET, analyzed levels of H3K79me3 and p53 in hepatic cells and in mice livers with carcinogenesis. Results suggested that SET partially suppressed H3K79me3 and interfering with DNA response via mediating down-regulation of p53 under treatment of TCE. To further figure out the regulatory cascade, DoT1L (regulator of H3K79me3) and p53 were knocked down in L-02 cells respectively. Additionally, extent of DNA damage along with alterations of H3K79me3 and p53 were also evaluated. Results indicated that reduced H3K79me3 could lead to down-regulation of p53 which further exacerbated TCE-induced DNA injury. These findings demonstrated that SET-H3K79me3-p53 served as an epigenetic regulatory axis involved in TCE-induced DNA damage response.
Trichomes on Cotton Leaf Surface Affect the Feeding Behaviors of Cotton Aphids
Authors: Yang Muzhi , Liu Chunyan , Zhao Tianyi , Li Yichao , Liu Huayan , Ren Zhentao , Xue Kun 
Published: January 2023

Abstract
Aphis gossypii Glover is one of the most important pests in cotton seedling. The specialized mouthpart and short generation time allow them seriously restricted the quality and yield of cotton. With the electrical penetration graph (EPG), optical microscope, and scanning electron microscope, the physical characteristics of trichomes on the cotton leaf surface and their effects on the feeding behaviors of cotton aphids were investigated in this study. Two types of leaf trichomes, glandular trichomes, and asteroid non-glandular trichomes were observed on the surface of cotton leaves under optical and scanning electron microscopes. As a whole, EPG results mainly showed differences in a non-probe waveform which meant searching on the leaf surface, and a potential drop waveform which meant intracellular penetration. Whereas pathway waveform, xylem waveform, and phloem waveform showed inconspicuous differences. Detailed indicators were slightly different at different periods. Our results confirmed that the structure of trichomes may directly affect the searching behaviors of herbivorous insect pests and hence regulate the plant-environment interactions.
Prediction of construction material prices using ARIMA and multiple regression models
Authors: Hosny Suad , Elsaid Elshaimaa , hosny Hossam 
Published: January 2023

Abstract
Construction Material Prices (CMP) variations have become a major issue in properly budgeting construction projects. Inability to accurately forecast CMP volatility can also lead to price overestimation or underestimation. Enhancing the accuracy of predictions of CMP can also enhance the accuracy of predictions of total construction costs. The purpose of this study is to present a model for predicting construction material prices that assist decision-makers to make better decisions over the life cycle of a project. The price records for CMP namely; steel, cement, brick, ceramic, and gravel, and the indicators affecting them in Egypt were used for the prediction procedures. The practical methods for using the Box-Jenkins approach Autoregressive Integrated Moving Average (ARIMA) time series and multiple regression models for forecasting building material prices are outlined in this research. Out-of-sample predictions are used to evaluate the provided model's performance in predicting future prices. The models are compared according to the Mean Absolute Percentage Errors (MAPE). The generated models show good results in predicting month-to-month variations in material prices, with MAPE ranging from 1.4 to 2.8 percent for the selected models. This research can assist both owners and contractors in improving their budgeting processes, and preparing more accurate cost estimates.
Simple and selective extraction of quercetin with MEPS method using modified glass powder by a molecularly imprinted polymer followed by spectrophotometric determination
Authors: sharifi Arman , hallaj Rahman , bahar Soleiman 
Published: January 2023

Abstract
A new sample preparation method based on microextraction in packed syringe (MEPS) was
developed for preconcentration of quercetin prior to its spectrophotometric determination. Molecularly imprinted polymers (MIPs) as packing material was used for higher extraction efficiency. First, Glass powder as support material because of low-cost and available substrate were modified and then MIPs synthesized by the sol-gel method using 3 aminopropyltriethoxysilane (APTES) as a functional monomer and tetraethyl orthosilicate (TEOS) as cross-linker. The combination of a molecularly imprinted polymers (MIPs) and microextraction in packed syringe (MEPS) increased the selectivity and sensitivity. The surface morphology and functionality of the prepared MIPs was characterized using Fourier-transform infrared spectroscopy (FTIR), field emission scanning electron microscopy (FESEM) and Thermogravimetric analysis (TGA). Different influencing parameters on extraction efficiency such as effect of the number of sample sorption/desorption cycles, type and volume of desorption solvent, pH of the sample solution and MIP amounts were optimized. Under the optimum condition, the proposed method displayed a linear range from 0.01 to 5 μg mL-1 and detection limit of 3.68 ng mL-1. Relative standard deviations (RSD) for 3 replicate determination of 1 μg mL-1 quercetin was 2.1 %. The proposed method was applied successfully for the selective extraction of quercetin from tea and coffee samples.
Hair cortisol as a biomarker for mood in bipolar disorder: a pilot study
Authors: Maimon Lior , Milo Tomer , Haran Dafna , Cohen Ben , Danon Tamar , Bren Anat , Mayo Avi , Rappaport Gadi Cohen , Alon Uri 
Published: January 2023

Abstract
Background
Bipolar disorder (BD) is characterized by episodes of manic, depressive and mixed mood states which have profound effects on the patient's quality of life. BD is diagnosed and followed up clinically using psychiatric assessment, with currently no biomarkers in clinical use for diagnosis or follow-up of the disorder. One potential biomarker is the stress hormone cortisol, but this has not been tested by comparing mood and cortisol within a cohort of people with BD. Here we asked whether hair cortisol might serve as a biomarker for mood in BD, with its advantage of averaging cortisol over months, bypassing concerns of circadian rhythm and acute stresses.
Methods
We measured cortisol in 2cm hair segments proximal to the scalp, which correspond to two months of hair growth. At the time hair was harvested, participants with BD were scored for depression and anxiety using the Hamilton Depression Rating Scale (HDRS), Beck Depression Inventory (BDI) and Hamilton Anxiety Rating Scale (HAM-A). We calculated partial Pearson correlations between mood scales and hair cortisol levels, accounting for participant’s age, gender, family status, education and medication.
Results
Hair cortisol correlated positively with mood scores in all three scales (r = 0.45–0.55, n = 38, p < = 0.01).
Conclusions
We conclude that hair cortisol may be a useful candidate biomarker for mood of BD patients over recent months.