bluuebunny/crossref_metadata_2025_split huggingface dataset:
The complexity and dynamics ofin organellotranslation assessed by high-resolution mitochondrial ribosome profiling
Authors: Wakigawa Taisei , Mito Mari , Yamashiro Haruna , Tomuro Kotaro , Tani Haruna , Tomizawa Kazuhito , Chujo Takeshi , Nagao Asuteka , Suzuki Takeo , Wei Fan-Yan , Shichino Yuichi , Suzuki Tsutomu , Iwasaki Shintaro 
Published: July 2023

AbstractSince mitochondrial translation serves the essential subunits of the OXPHOS complex that produces ATP, exhaustive, quantitative, and high-resolution delineation of mitoribosome traversal is needed. Here, we developed a technique for high-resolution mitochondrial ribosome profiling and revealed the intricate regulation of mammalsin organellotranslation. Our approach assessed the stoichiometry and kinetics of mitochondrial translation flux, such as the number of mitoribosomes on a transcript and the elongation rate, initiation rate, and lifetime rounds of translation of individual transcripts. We also surveyed the impacts of modifications at the anticodon stem loop in mt-tRNAs, including all possible modifications at the 34th position, by deleting the corresponding enzymes and harnessing patient-derived cells. Moreover, a retapamulin-assisted derivative and mito-disome profiling revealed cryptic translation initiation sites at subcognate codons and programmed mitoribosome collision sites across the mitochondrial transcriptome. Our work provides a useful platform for investigating protein synthesis within the energy powerhouse of the cell.
Cytosolic linear DNA plasmids inSaccharomycopsisspecies
Authors: Cinnéide Eoin Ó , Heneghan Padraic G. , Rajkumar Arun S. , Morrissey John P. , Wolfe Kenneth H. 
Published: July 2023

AbstractSome budding yeast species contain cytosolic linear DNA plasmids (also called virus-like elements, VLEs) that code for killer toxins that can kill other yeasts. The toxins are anticodon nucleases that cleave a specific tRNA in the cells being attacked, stopping translation. The best known plasmids of this type are the pGKL1/pGKL2 system ofKluyveromyces lactis. pGKL1 is a killer plasmid encoding the toxin zymocin (γ-toxin) which cleaves tRNA-Glu, and pGKL2 is a helper plasmid required for replication and transcription of pGKL1. Here, we investigated similar plasmids in the genusSaccharomycopsisthat were originally described in the 1980s.Saccharomycopsishas undergone an evolutionary change of its genetic code, from CUG-Leu to CUG-Ser translation, which we hypothesized could have been driven by a tRNA-cleaving toxin encoded by a cytosolic plasmid. We sequenced a three-plasmid system inS. crataegensis, consisting of apparent killer, immunity, and helper plasmids. The killer plasmid contains genes coding for putative α/β (chitin-binding) and γ (ribonuclease) toxin subunits, but the γ-toxin gene is damaged in all the isolates we examined. We inferred the sequence of the intactS. crataegensisγ-toxin and expressed it inSaccharomyces cerevisiaeandKluyveromyces marxianus, but it did not cause a growth defect. We also identified free plasmids, or plasmids integrated into the nuclear genome, in nine otherSaccharomycopsisspecies, including a case of recent interspecies transfer of a plasmid. Our results show that many yeasts in the CUG-Ser2 clade contain, or have in the past contained, plasmids related to those that carry anticodon nucleases.
Characterization of thePlasmodium bergheiregulatory AAA-ATPase subunit Rpt3 as an activator of Protein Phosphatase 1: direct and indirect evidence
Authors: Lainé Claudianne , De Witte Caroline , Martoriati Alain , Farce Amaury , Metatla Inès , Guerrera Ida Chiara , Cailliau Katia , Khalife Jamal , Pierrot Christine 
Published: July 2023

AbstractThe 26S proteasome is the main proteolytic machine involved in protein degradation, thus contributing to homeostasis or stress response of eukaryotic cells. This macromolecular complex, consisting of a 20S core particle assembled with one or two 19S regulatory particles, is highly regulated by phosphorylation. Here we describe thePlasmodium bergheiproteasome AAA-ATPase regulatory subunit Rpt3 and show that it binds to protein phosphatase 1, the major parasite phosphatase. In addition, PbRpt3 regulates the activity of the phosphatase both in vitro and in a heterologous model ofXenopusoocytes. Using mutagenesis approaches, we observed that the RVXF motifs of PbRpt3 are involved in this binding and activity. Further use ofXenopusoocyte model and mutagenesis based on the 3D model that we established revealed that the binding capacity of PbRpt3 to ATP may also contribute to its phosphatase-regulating activity. In the parasite, reverse genetic studies suggested an essential role for PbRpt3 since no viable knock-out line could be obtained. Additionally, immunoprecipitation assays followed by mass spectrometry analyses using transgenic PbRpt3-tagged parasites not only confirmed that PbRpt3 belongs to the 19S regulatory particle of the proteasome, but also revealed potential interaction with proteins already shown to play a role in the phospholipid membrane dynamics.
Loss of REP1 impacts choroidal melanogenesis in choroideremia
Authors: Sakar Hajrah , Tracey-White Dhani , Hagag Ahmed M. , Burgoyne Thomas , Jensen Lasse D. , Edwards Malia M. , Moosajee Mariya 
Published: July 2023

AbstractChoroideremia (CHM) is a rare X-linked chorioretinal dystrophy affecting the photoreceptors, retinal pigment epithelium (RPE) and choroid, however, the involvement of the choroid in disease progression is not fully understood. CHM is caused by mutations in theCHMgene, encoding the ubiquitously expressed Rab escort protein 1 (REP1). REP1 plays an important role in intracellular trafficking of vesicles, including melanosomes. In this study, we examined ultrastructure of the choroid inchmru848fish andChmnull/WTmouse models using transmission electron microscopy. Significant pigmentary disruptions were observed, with a lack of melanosomes in the choroid ofchmru848fish from 4 days post fertilisation (4dpf). Total melanin and expression of melanogenesis genestyr,tryp1a,mitf, dctandpmelwere also reduced from 4dpf. InChmnull/WTmice, choroidal melanosomes were significantly smaller at 1 month and at 1 year, eumelanin was reduced, and the choroid was thicker. The choroid in CHM patients was also examined using optical coherence tomography (OCT) and OCT- angiography (OCT-A) and the area of preserved choriocapillaris (CC) was found to be smaller than that of overlying photoreceptors, indicating that the choroid is degenerating at a faster rate. Histopathology of an enucleated eye from a 74-year-old CHM male patient revealed isolated areas of RPE but no associated underlying CC. Significant degenerative changes in the choroid of CHM patients and animal models are seen, highlighting the importance of administrative routes involving the choroid, such as suprachoroidal delivery. Pigmentary disruptions in CHM animal models reveal an important role for REP1 in melanogenesis, and drugs that improve melanin production represent a potential novel therapeutic avenue.
The hGIDGID4E3 ubiquitin ligase complex targets ARHGAP11A to regulate cell migration
Authors: Bagci Halil , Winkler Martin , Uliana Federico , Boulais Jonathan , Mohamed Weaam I , Park Sophia L , Côté Jean-François , Peter Matthias 
Published: July 2023

AbstractThe human CTLH/GID (hGID) complex emerged as an important E3 ligase regulating multiple cellular processes, including cell cycle progression and metabolic activity. However, the range of biological functions controlled by hGID remains unexplored. Here, we show that the hGID substrate receptor GID4 regulates cell growth and migration. Biochemical and cellular assays combined with proximity-dependent biotinylation (BioID2) revealed that the hGIDGID4E3-ligase targets the Rho-GAP ARHGAP11A for degradation. Depletion of GID4 or impeding the GID4 substrate binding pocket impairs motility and directed cell movement, whereas knockdown of ARHGAP11A significantly restores the cell migration defect. We found that GID4 controls cell migration by degrading ARHGAP11A thereby preventing its accumulation at the cell periphery where it inactivates RhoA activity. Together, we identified a unique function for GID4, as well as a wide range of substrate profiles beyond Pro/N-degron motifs, which pave the way for deciphering additional pathways regulated by hGID E3 ligase activity through its GID4 substrate receptor.
Hybrid strategy and firm performance: a time-series qualitative comparative analysis of the Chinese ICT sector
Authors: Tao Yan , Ke Hongyan , Zhang Ziye 
Published: July 2023

PurposeThe paper examines whether the hybrid strategy can generate high performance and what hybrid strategy configurations are more conducive to high performance.Design/methodology/approachThis paper explores the complex causal relationships between six strategic elements (marketing, growth, R&D, capital, efficiency, stability) and firm performance. From a configurational approach, the authors utilize necessary condition analysis, time-series qualitative comparative analysis, and typical case extraction techniques to analyze 944 balanced panel data from 118 Chinese ICT firms during 2013–2020.FindingsChinese ICT sector firms do not rely on pure strategies (prospector or defender) to achieve high performance. The hybrid strategy is conducive to high performance. Only specific hybrid strategy configurations, including stable growth, innovative efficiency, and two-way player types, could enable firms to perform well. Six strategic elements do not constitute a necessary condition for high performance.Originality/valueThis paper proposed an integrated qualitative comparative analysis scheme, proved the effectiveness of the hybrid strategy on firm performance, and revealed how hybrid strategy configurations generate high performance.
Diminishing returns or inverted U? The curvilinear relationship between eco-innovation and firms' sustainable business performance: the impact of market turbulence
Authors: Xuhua Hu , Larbi-Siaw Otu , Thompson Erika Tano 
Published: July 2023

PurposeEco-innovations (EIs) are intended to benefit not only the environment but society and firms, but how the relationship is reconciled is unclear, particularly in emerging economies. The advancement of EI has resulted in both positive and negative relationships with sustainability, indicating that the association is more complex than a simple linear one.Design/methodology/approachThus, the authors hypothesize that EI has a curvilinear relationship with sustainable business performance (SPB) and that market turbulence (MT) exerts stimulus that reinforces EIs. Accordingly, using the Stata software, the authors apply a moderated regression to a sample size data of 511 manufacturing firms to test the hypothesized assumptions.FindingsAlthough the results attest to a positive relationship between EI and SBP, the results are synonymous with an inverted “U” shape that renders EIs unprofitable beyond a certain threshold (rebound effect). Additionally, the authors observe that the moderation stimulus of technology turbulence flattens the inverted U-shaped curve.Originality/valueBuilt on the foundations of natural-resource-based view (NRBV) and contingency theory, the authors identify the rebound effect point of EI and SBP and the reinforcing stimulus of MT.
Minor‐focused sextortion by adult strangers: A crime script analysis of newspaper and court cases
Authors: O'Malley Roberta Liggett , Holt Karen , Holt Thomas J. , Rodriguez Joy 
Published: July 2023

AbstractSummarySextortion is a form of image‐based sexual abuse in which an offender threatens to distribute sexually explicit images, videos, or information to gain victim compliance. In some sextortion incidents involving minor children, offenders are adult strangers who coerce victims into self‐producing child sexually abusive materials (CSAM). Despite the growing concern regarding minor victims of sextortion, there is a lack of empirical investigation of adult offenders who exclusively target minor children for the production of CSAM and the steps and decisions these offenders take to commit sextortion. Utilizing a sample of 130 minor‐focused sextortion cases gathered from news media and court documents, the current study employed crime script analysis and inductive qualitative methodology to investigate how adult offenders commit sextortion. The results indicated that adult offenders specifically targeting minor children move through several crime scripts: preparation, entry, instrumental actions, crime commission, and exit.Policy ImplicationsMinor‐focused sextortion perpetrated by adult offenders is executed in stages, each with accompanying preventive solutions. Investing in education that teaches youth to recognize grooming, develops safety practices online, and removes barriers to reporting should be a priority for schools and parents. Increased adoption of technology and policies that would allow social media platforms to detect CSAM, remove fraudulent accounts, and streamline reporting would also disrupt these crimes.
Epidemiological characteristics of cases with scrub typhus and their correlation with chigger mite occurrence (2019–2021): A focus on case occupation and activity locations
Authors: Jeong Se‐Jin , Jeon Jin‐Hwan , Hwang Kyung won 
Published: July 2023

AbstractScrub typhus, transmitted through chigger mite bites, is a febrile disease associated with agricultural activities and primarily occurs in autumn. To address the increasing number of cases with scrub typhus over the past 3 years, we analyzed activity, visit history, and occupation data of 12,462 cases reported to the Korea Disease Control and Prevention Agency (KDCA) between January 2019 and December 2021. We examined changes in incidence and general characteristics by visualizing the incidence and eschar bite locations using population data and a geographic information system. Gyeongsangnam‐do had the highest overall incidence rate (22.7%), followed by Jeollanam‐do (19.0%) and Jeollabuk‐do (11.2%), during the study period. However, when considering the incidence rate per 100,000 people, Jeollanam‐do had the highest number of cases at 129.1, followed by Gyeongsangnam‐do (85.1 cases) and Jeollabuk‐do (78.1 cases). Most cases were women (61.0%) in their 70s (28.4%), and the highest incidence occurred in the agricultural worker group during autumn (October–December). Bites occurred most frequently in the abdomen (18.1%), and fever (79.2%) and muscle pain (49.8%) were the main symptoms. We also found a positive correlation (r = 0.848) between chigger mite populations and disease outbreaks over 3 years. In conclusion, it is crucial to provide education on identifying the habitats of chigger mites and rodents (vectors), as well as the appropriate application of repellents. Promoting awareness and preventive measures against infectious diseases is of utmost importance for rural areas and the general public who frequently come into contact with natural environments.
Measures of fetal growth and preterm birth and risk of endometriosis and adenomyosis in adult life: a systematic review and meta‐analysis
Authors: Brønd Marie , Breintoft Kjerstine , Forman Axel , Henriksen Tine Brink , Ramlau‐Hansen Cecilia Høst , Rytter Dorte , Arendt Linn Håkonsen 
Published: July 2023

AbstractIntroductionExposures in utero are suggested to play a role in the etiology of endometriosis and adenomyosis, although the current evidence is inconclusive. Knowledge about potential prenatal programming and early life exposures that may affect this risk is of high importance, to focus potential preventive strategies for the diseases already during pregnancy. The aim of this study was to review systematically the literature of the association between measures of fetal growth and preterm birth and endometriosis and adenomyosis in adult life.Material and methodsA systematic review according to Preferred Reporting Items for Systematic Reviews and Meta‐analysis (PRISMA) guidelines and by search on PubMed and EMBASE was carried out. We included published case–control and cohort studies. We excluded studies without a reference group, eg case series, case reports as well as commentaries, letters and editorials. The quality of the studies was assessed using the Newcastle–Ottawa Scale. Meta‐analyses using a random‐effect inverse variance weighted model were performed. PROSPERO registration number is CRD42021249322.ResultsA total of 11 studies were included. In general, the quality scores of the studies were moderate. We found that the risk of endometriosis was 26% higher in women born with a birthweight <2.5 kg (pooled odds ratio [pOR] 1.26, 95% confidence interval [CI] 1.05–1.52) and 32% higher in women born preterm (pOR 1.32, 95% CI 1.01–1.72) than in the reference groups. The studies on adenomyosis pointed towards no association, but a meta‐analysis was unfeasible due to the small number of studies.ConclusionsThis systematic review and meta‐analysis found that low birthweight and being born preterm were associated with endometriosis in adult life, but the results must be interpreted cautiously. No solid conclusion could be made regarding adenomyosis due to a limited number of published studies, but the studies included found no association. The results support the hypothesis of a potential early programming effect of endometriosis. However, the body of evidence is sparse and this hypothesis needs to be investigated further.
Three‐dimensional endovaginal ultrasound assessment using the levator ani deficiency score in primiparas: A replication study
Authors: Rotstein Emilia , Ullemar Vilhelmina , Starck Marianne , Tegerstedt Gunilla 
Published: July 2023

AbstractIntroductionIt is essential to assess the levator ani properly as part of clinical care in patients presenting with pelvic floor dysfunction. The levator ani deficiency scoring system is a previously published method to assess levator ani defects with three‐dimensional endovaginal ultrasound. The primary aim of this study was to determine the intra‐ and interrater reliability of the levator ani deficiency score in a cohort of non‐instrumentally delivered primiparas.Material and methodsPrimiparas (n = 141) were examined at least 1 year after vaginal birth. Three‐dimensional endovaginal ultrasound volumes were acquired by a single examiner using two different automated ultrasound probes. The volumes were analyzed by two separate raters who were blinded to each other's assessments. Descriptive statistics were calculated for levator ani deficiency score and categorized into three levels (mild, moderate, severe). Kendall's tau‐b was calculated for intra‐ and interrater comparisons.ResultsIntrarater comparisons of levator ani deficiency score and levator ani deficiency category were high (Kendall's tau‐b ≥0.80 for Rater 1; >0.79 for Rater 2). Interrater comparisons of levator ani deficiency score and levator ani deficiency category were also high (Kendall's tau‐b >0.9 for assessment 1 and >0.78 for assessment 2). Varying by rater, probe and assessment, 75.9%–80.1% of the study population had no/mild deficiency, 6.4%–9.2% had moderate deficiency, and 4.3%–6.4% had severe levator ani deficiency.ConclusionsThe levator ani deficiency scoring system is a feasible method to assess defects of the levator ani muscle and can be reproduced with high intra‐ and interrater correlations. Using the scoring system in clinical practice may facilitate concordant assessment between different examiners. However, the system should be used to support clinical findings and symptomatology and not as a screening tool, as the score is lacking the category of no levator ani deficiency.
Photobiomodulation reduces neuropathic pain after spinal cord injury by downregulating CXCL10 expression
Authors: Zhang Zhihao , Zhu Zhijie , Zuo Xiaoshuang , Wang Xuankang , Ju Cheng , Liang Zhuowen , Li Kun , Zhang Jiawei , Luo Liang , Ma Yangguang , Song Zhiwen , Li Xin , Li Penghui , Quan Huilin , Huang Peipei , Yao Zhou , Yang Ning , Zhou Jie , Kou Zhenzhen , Chen Beiyu , Ding Tan , Wang Zhe , Hu Xueyu 
Published: July 2023

AbstractBackgroundMany studies have recently highlighted the role of photobiomodulation (PBM) in neuropathic pain (NP) relief after spinal cord injury (SCI), suggesting that it may be an effective way to relieve NP after SCI. However, the underlying mechanisms remain unclear. This study aimed to determine the potential mechanisms of PBM in NP relief after SCI.MethodsWe performed systematic observations and investigated the mechanism of PBM intervention in NP in rats after SCI. Using transcriptome sequencing, we screened CXCL10 as a possible target molecule for PBM intervention and validated the results in rat tissues using reverse transcription‐polymerase chain reaction and western blotting. Using immunofluorescence co‐labeling, astrocytes and microglia were identified as the cells responsible for CXCL10 expression. The involvement of the NF‐κB pathway in CXCL10 expression was verified using inhibitor pyrrolidine dithiocarbamate (PDTC) and agonist phorbol‐12‐myristate‐13‐acetate (PMA), which were further validated by an in vivo injection experiment.ResultsHere, we demonstrated that PBM therapy led to an improvement in NP relative behaviors post‐SCI, inhibited the activation of microglia and astrocytes, and decreased the expression level of CXCL10 in glial cells, which was accompanied by mediation of the NF‐κB signaling pathway. Photobiomodulation inhibit the activation of the NF‐κB pathway and reduce downstream CXCL10 expression. The NF‐κB pathway inhibitor PDTC had the same effect as PBM on improving pain in animals with SCI, and the NF‐κB pathway promoter PMA could reverse the beneficial effect of PBM.ConclusionsOur results provide new insights into the mechanisms by which PBM alleviates NP after SCI. We demonstrated that PBM significantly inhibited the activation of microglia and astrocytes and decreased the expression level of CXCL10. These effects appear to be related to the NF‐κB signaling pathway. Taken together, our study provides evidence that PBM could be a potentially effective therapy for NP after SCI, CXCL10 and NF‐kB signaling pathways might be critical factors in pain relief mediated by PBM after SCI.
Perspective: Is re‐interpretation enough? Dismantling violence in the natural history museum
Authors: Heller Nicole E. 
Published: July 2023

AbstractThe taxidermy assemblage “Lion Attacking a Dromedary” (LAD) has been critiqued as a piece of French colonial propaganda, containing unethically sourced human remains, that desensitizes visitors to violence against people of color and lacks value for science education. It has also been on display continuously since 1899 at the Carnegie Museum of Natural History and has sentimental and pedagogical value for local community, thus making it complicated to remove. To address its difficult legacy while maintaining its display, LAD has been re‐interpreted multiple times with new descriptive labels. This approach is of limited success, however. Re‐interpretation cannot sufficiently counter the visual narrative of the diorama itself. In addition to the aforementioned critiques, the figures in LAD are posed to tell a harmful “man versus nature” story. LAD points toward a broader narrative bias in natural history, namely an overemphasis on competition and an underemphasis on cooperation. For ethical and effective contemporary science education, and for galvanizing action on climate change and other sustainability and justice crises in the 21st‐century natural history museum, there is an urgent need for radical deconstruction of dioramas like LAD.