bluuebunny/crossref_metadata_2025_split huggingface dataset:
Enzymatic post-treatment of ozonation: laccase-mediated removal of the by-products of acetaminophen ozonation
Authors: Schmiemann Dorothee , Hohenschon Lisa , Bartels Indra , Hermsen Andrea , Bachmann Felix , Cordes Arno , Jäger Martin , Gutmann Jochen Stefan , Hoffmann-Jacobsen Kerstin 
Published: March 2023

AbstractOzonation is a powerful technique to remove micropollutants from wastewater. As chemical oxidation of wastewater comes with the formation of varying, possibly persistent and toxic by-products, post-treatment of the ozonated effluent is routinely suggested. This study explored an enzymatic treatment of ozonation products using the laccase from Trametes versicolor. A high-performance liquid chromatography coupled with high-resolution mass spectrometry (HPLC-HRMS) analysis revealed that the major by-products were effectively degraded by the enzymatic post-treatment. The enzymatic removal of the by-products reduced the ecotoxicity of the ozonation effluent, as monitored by the inhibition of Aliivibrio fischeri. The ecotoxicity was more effectively reduced by enzymatic post-oxidation at pH 7 than at the activity maximum of the laccase at pH 5. A mechanistic HPLC-HRMS and UV/Vis spectroscopic analysis revealed that acidic conditions favored rapid conversion of the phenolic by-products to dead-end products in the absence of nucleophiles. In contrast, the polymerization to harmless insoluble polymers was favored at neutral conditions. Hence, coupling ozonation with laccase-catalyzed post-oxidation at neutral conditions, which are present in wastewater effluents, is suggested as a new resource-efficient method to remove persistent micropollutants while excluding the emission of potentially harmful by-products.
Graphical abstract
Mesoporous Ag-functionalized magnetic activated carbon-based agro-waste for efficient removal of Pb(II), Cd(II), and microorganisms from wastewater
Authors: Ali Omnia I. , Zaki Eman R. , Abdalla Mohga S. , Ahmed Saber M. 
Published: March 2023

Abstract
Herein, eco-friendly mesoporous magnetic activated carbon-based agro-waste nanosorbents incorporating antimicrobial silver nanoparticles (Mag@AC1-Ag and Mag@AC1-Ag) have been prepared. Various techniques (XRD, SEM/EDX, TEM, FTIR, and BET analysis) were employed to characterize the prepared nanosorbents before being utilized as novel nanosorbents to remove Pb+2 and Cd+2 ions. Mag@AC1-Ag and Mag@AC1-Ag exhibited rapid and excellent uptake of Pb+2 and Cd+2. The pseudo-second-order kinetics and the Langmuir isotherm are more suitable for the explanation of the experimental results. The thermodynamic parameters showed that the Pb+2 and Cd+2 sorption by the nanosorbents was a spontaneous and endothermic reaction. The prepared nanosorbents can be effectively regenerated using HCl and recycled up to the fifth cycle. These nanosorbents’ potential uses for eliminating Pb+2 and Cd+2 from real water samples were evaluated. Moreover, the results revealed that both Mag@AC1-Ag and Mag@AC2-Ag exhibited high antimicrobial activity against fecal coliform (gram-negative) and Bacillus subtilis (gram-positive).
Population growth of phosphine resistant and susceptible populations of Lasioderma serricorne (F.) (Coleoptera:Anobiidae) exposed to different temperatures and commodities
Authors: Sakka Maria K. , Athanassiou Christos G. 
Published: March 2023

Abstract
The aim of this work was to investigate the population growth of Lasioderma serricorne (F.) with two populations with different susceptibility to phosphine (one resistant and one susceptible). Population growth was recorded on different days (35 days, 50 days, 65 days, 80 days, 95 days, and 110 days) in two different commodities: (a) mixed food consisted of wheat flour (10 parts) + cornmeal (10 parts) + brewers’ yeast (1.5 parts) and (b) wheat flour. Our results clearly indicate that both populations preferred mixed food compared to wheat flour for all combinations tested. Moreover, the increase in temperature from 25 to 30 °C showed a positive effect in some combinations in the population growth of both populations. In general, we found some differences in the production of offspring between the susceptible and the resistant population. Based on the results of the present study, population growth may provide critical information for the fitness advantages or disadvantages of each population.
Managerial marketing and behavioral marketing: when myths about marketing management and consumer behavior lead to a misconception of the discipline
Authors: Bruhn Manfred , Gröppel-Klein Andrea , Kirchgeorg Manfred 
Published: March 2023

AbstractMarketing has become accepted and proven in science and practice over the past decades. Many principles of marketing have become self-evident, but have led to gross generalizations and simplified thought patterns. This article examines myths and metaphors in marketing. A distinction is made between myths of managerial marketing and of behavioral marketing. In both sub-disciplines, we are often dealing with complex and invisible phenomena that occur in a variety of contexts. Attempts to explain these phenomena are therefore particularly prone to the emergence of myths or misconceptions. From a managerial perspective, topics such as the philosophy and leadership role of marketing, the generation of innovation success, the development of strategic competitive advantages, and brand management are reflected. Since the change from a sellers’ market to a buyers’ market, consumer behavior research and marketing have been closely intertwined. Therefore, findings from consumer behavior research form a central basis for successful decisions in marketing management. Based on this background, the second part of the article focuses on myths of consumer behavior research, here addressing unconscious phenomena in consumer behavior, mainly the discussion of subliminal priming (and its impact on manipulation) and unconscious perception as well as of unconscious motives, and approaches to implicite attitude measurement. Finally, implications are derived as to what extent the presented phenomena and the unveiling of myths have an impact on marketing management and on consumer behavior research and what roles these disciplines should take in the future. In the era of climate change and digital transformation, particular challenges are emerging. Thus, facts and not myths should determine the future path of the marketing discipline.
A consumer perspective on Corporate Digital Responsibility: an empirical evaluation of consumer preferences
Authors: Carl K. Valerie , Mihale-Wilson Cristina , Zibuschka Jan , Hinz Oliver 
Published: March 2023

AbstractWhile digitalization offers numerous new possibilities for value creation, managers have to overcome a number of threats and obstacles that it harbors. In this context, the concept of Corporate Digital Responsibility (CDR) is of increasing interest to practitioners. Drawing on the well-established paradigm of Corporate Social Responsibility, CDR comprises a set of principles designed to encourage the ethical and conscientious development, adoption, and utilization of digital technologies. This work aims at contributing to the evolving research base by empirically assessing consumer preferences and a consumer segmentation approach with regard to companies’ concrete CDR activities, thus supporting the operationalization of CDR. Hence, this work provides concrete guidance for firms’ CDR activities in practice. To this end, a series of Best–Worst Scaling and dual response studies with a representative sample of 663 German-speaking participants assesses consumers’ perspectives on firms’ concrete (possible) activities within several CDR dimensions. Both DURE studies reveal the potential halo effect of data privacy and security activities on the perception of the CDR engagement at large, suggesting a more holistic approach to digital responsibilities. Besides, the findings reveal that in case of CDR one size does not fit all. Especially in terms of informational approaches, consumer preferences are rather heterogeneous suggesting that consumer segmentation is beneficial for companies. Additionally, the high importance of price for the consumers’ evaluation shows that it can be useful to offer a slimmed-down version in terms of CDR activities for more price-conscious consumers.
Red Alarm for Pre-trained Models: Universal Vulnerability to Neuron-level Backdoor Attacks
Authors: Zhang Zhengyan , Xiao Guangxuan , Li Yongwei , Lv Tian , Qi Fanchao , Liu Zhiyuan , Wang Yasheng , Jiang Xin , Sun Maosong 
Published: March 2023

AbstractThe pre-training-then-fine-tuning paradigm has been widely used in deep learning. Due to the huge computation cost for pre-training, practitioners usually download pre-trained models from the Internet and fine-tune them on downstream datasets, while the downloaded models may suffer backdoor attacks. Different from previous attacks aiming at a target task, we show that a backdoored pre-trained model can behave maliciously in various downstream tasks without foreknowing task information. Attackers can restrict the output representations (the values of output neurons) of trigger-embedded samples to arbitrary predefined values through additional training, namely neuron-level backdoor attack (NeuBA). Since fine-tuning has little effect on model parameters, the fine-tuned model will retain the backdoor functionality and predict a specific label for the samples embedded with the same trigger. To provoke multiple labels in a specific task, attackers can introduce several triggers with predefined contrastive values. In the experiments of both natural language processing (NLP) and computer vision (CV), we show that NeuBA can well control the predictions for trigger-embedded instances with different trigger designs. Our findings sound a red alarm for the wide use of pre-trained models. Finally, we apply several defense methods to NeuBA and find that model pruning is a promising technique to resist NeuBA by omitting backdoored neurons.
One-pot synthesis of N-doped carbon dots from microwave-irradiated egg white: application to raspberry ketone assay by photo-induced charge transfer fluorescence sensing
Authors: Abdelaal Sarah H. , El-Kosasy Amira M. , Abdelrahman Mona H. 
Published: March 2023

AbstractIn this article, we designed one-step economic eco-harmonious microwave-assisted procedure to prepare nitrogen-doped carbon dots. We selected egg white as a cheap glycoprotein-based carbon source without the assistance of any chemicals. The synthetic process requires only 3 min during which carbonization and nitrogen doping are realized at the same time. The fabricated carbon dots were characterized for particle size, structure and photoluminescence behaviour. The nanodots were amorphous carbon-rich naturally nitrogen-doped particles with plentiful attached hydrophilic functional groups. They had average particle size 2.98 ± 1.57 nm, emitted strong blue fluorescence and showed excitation-dependant emission behaviour. What is more, the practical use of this system for raspberry ketone determination in commercially available weight loss dietary supplement product is demonstrated successfully. In ethylene glycol medium, the addition of raspberry ketone enhances the emission intensity of the synthesized carbon dots. The effect of reaction time and solvent was investigated. After optimization, the intensity enhancement was linear to the amount of raspberry ketone added to the assay solution in the concentration range of 100–1000 ng/ml, with detection and quantitation limits of 15.10 and 45.45 ng/ml, respectively. The method was validated in accordance to International Conference on Harmonization (ICH) guidelines and further applied to raspberry ketone capsules showing excellent results.
Graphical abstract
HucMSC-EVs Facilitate In Vitro Development of Maternally Aged Preantral Follicles and Oocytes
Authors: Zhang Ying-Yi , Yang Weijie , Zhang Yi , Hu Zhanhong , Chen Yingyan , Ma Yerong , Yang Anran , Shi Zhan , Zhou Hanjing , Ren Peipei , Shi Libing , Jin Jiamin , Rong Yan , Tong Xiaomei , Zhang Yin-Li , Zhang Songying 
Published: March 2023

AbstractFollicle developmental capacity and oocyte quality decline with advanced maternal age. Extracellular vesicles from human umbilical cord mesenchymal stem cells (HucMSC-EVs) act as a potential therapeutic product in the treatment of age-related ovarian dysfunction. In vitro culture (IVC) of preantral follicles is a useful method for understanding the mechanism of follicle development and is a promising means for improving female fertility. However, whether HucMSC-EVs have beneficial effects on aged follicle development during IVC has not yet been reported. Our research demonstrated that follicular development with single-addition withdrawal of HucMSC-EVs was better than that with continuous treatment with HucMSC-EVs. HucMSC-EVs facilitated the survival and growth of follicles, promoted the proliferation of granulosa cells (GCs), and improved the steroid hormone secretion of GCs during IVC of aged follicles. Both GCs and oocytes could uptake HucMSC-EVs. Moreover, we observed elevated cellular transcription in GCs and oocytes after treatment with HucMSC-EVs. The RNA sequencing (RNA-seq) results further validated that the differentially expressed genes are related to the promotion of GC proliferation, cell communication, and oocyte spindle organization. Additionally, the aged oocytes displayed a higher maturation rate, presented less aberrant spindle morphology, and expressed a higher level of the antioxidant protein Sirtuin 1 (SIRT1) after treatment with HucMSC-EVs. Our findings suggested that HucMSC-EVs can improve the growth and quality of aged follicles and oocytes in vitro through the regulation of gene transcription, which provides evidence for HucMSC-EVs as potential therapeutic reagents to restore female fertility with advanced age.
Graphical Abstract
Modeling cancer’s ecological and evolutionary dynamics
Authors: Bukkuri Anuraag , Pienta Kenneth J. , Hockett Ian , Austin Robert H. , Hammarlund Emma U. , Amend Sarah R. , Brown Joel S. 
Published: March 2023

AbstractIn this didactic paper, we present a theoretical modeling framework, called the G-function, that integrates both the ecology and evolution of cancer to understand oncogenesis. The G-function has been used in evolutionary ecology, but has not been widely applied to problems in cancer. Here, we build the G-function framework from fundamental Darwinian principles and discuss how cancer can be seen through the lens of ecology, evolution, and game theory. We begin with a simple model of cancer growth and add on components of cancer cell competition and drug resistance. To aid in exploration of eco-evolutionary modeling with this approach, we also present a user-friendly software tool. By the end of this paper, we hope that readers will be able to construct basic G function models and grasp the usefulness of the framework to understand the games cancer plays in a biologically mechanistic fashion.
TGF-β Activated Kinase 1 (TAK1) Is Activated in Microglia After Experimental Epilepsy and Contributes to Epileptogenesis
Authors: Khan Dilaware , Bedner Peter , Müller Julia , Lülsberg Fabienne , Henning Lukas , Prinz Marco , Steinhäuser Christian , Muhammad Sajjad 
Published: March 2023

AbstractIncreasing evidence suggests that inflammation promotes epileptogenesis. TAK1 is a central enzyme in the upstream pathway of NF-κB and is known to play a central role in promoting neuroinflammation in neurodegenerative diseases. Here, we investigated the cellular role of TAK1 in experimental epilepsy. C57Bl6 and transgenic mice with inducible and microglia-specific deletion of Tak1 (Cx3cr1CreER:Tak1fl/fl) were subjected to the unilateral intracortical kainate mouse model of temporal lobe epilepsy (TLE). Immunohistochemical staining was performed to quantify different cell populations. The epileptic activity was monitored by continuous telemetric electroencephalogram (EEG) recordings over a period of 4 weeks. The results show that TAK1 was activated predominantly in microglia at an early stage of kainate-induced epileptogenesis. Tak1 deletion in microglia resulted in reduced hippocampal reactive microgliosis and a significant decrease in chronic epileptic activity. Overall, our data suggest that TAK1-dependent microglial activation contributes to the pathogenesis of chronic epilepsy.
Effective Fermentation of Sugarcane Bagasse Whole Slurries Using Robust Xylose-Capable Saccharomyces cerevisiae
Authors: Mokomele Thapelo , Brandt Bianca A. , Görgens Johann F. 
Published: March 2023

AbstractThe pre-treatment of lignocellulose material towards cellulosic bioethanol production releases microbial inhibitors that severely limit the fermentation ability of Saccharomyces cerevisiae. This study evaluated to what degree robust xylose-capable strains may improve the fermentability of non-detoxified sugarcane bagasse (SCB) slurries derived from steam explosion (StEX) and further compared this to slurries derived from ammonia fibre expansion (AFEX) pre-treatment. Initial screening in separate hydrolyses and co-fermentation processes using StEx-SCB hydrolysates identified S. cerevisiae TP-1 and CelluXTM4 with higher xylose consumption (≥ 88%) and ethanol concentrations (≥ 50 g/L), and ethanol metabolic yields (≥89% relative to theoretical maximum), even in the presence of approximately 8 g/L of acetic acid. Under industrially relevant pre-hydrolysis simultaneous saccharification and co-fermentation (PSSCF) conditions of high solids loading (15%, w/w) and low enzyme dosage (8 mg protein per gram untreated biomass), the fermentation of StEx-treated SCB whole slurry achieved ethanol yields of 208 and 224 L per Mg raw dry SCB using S. cerevisiae TP-1 and CelluXTM4, respectively. Under the same solids loading and enzyme dosages, the PSSCF of ammonia fibre expansion (AFEXTM) pre-treated SCB achieved ethanol yields of 234 and 251 L per Mg raw dry SCB using TP-1 and CelluXTM4, respectively. The study achieved non-detoxified whole-slurry co-fermentation using StEx pre-treated SCB, with higher ethanol yields than previously reported, by utilising robust xylose-capable strains. The results of this work provide insights into the potential use of inhibitor-tolerant S. cerevisiae strains TP-1 and CelluXTM4 as ethanologens for the fermentation of steam-exploded and undetoxified SCB whole slurries.
Finite element model to simulate impact on a soft tissue simulant
Authors: Imam Syed Adil , Hughes Angus C. , Carré Matthew J. , Driscoll Heather , Winwood Keith , Venkatraman Prabhuraj , Allen Tom 
Published: March 2023

AbstractA finite element model of an impact test on a soft tissue simulant, used as part of a shoulder surrogate, was developed in Ansys© LS-DYNA®. The surrogate consisted of a metal hemicylindrical core, with a diameter of 75 mm, covered with a 15 mm thick relaxed muscle simulant. The muscle simulant consisted of a 14 mm thick layer of silicone covered with 1 mm thick chamois leather to represent skin. The material properties of the silicone were obtained via quasi-static compression testing (curve fit with hyperelastic models) and compressive stress relaxation testing (curve fit with a Prony series). Outputs of the finite element models were compared against experimental data from impact tests on the shoulder surrogate at energies of 4.9, 9.8 and 14.7 J. The accuracy of the finite element models was assessed using four parameters: peak impact force, maximum deformation, impact duration and impulse. A 5-parameter Mooney-Rivlin material model combined with a 2-term Prony series was found to be suitable for modelling the soft tissue simulant of the shoulder surrogate. This model had under 10% overall mean deviation from the experimental values for the four assessment parameters across the three impact energies. Overall, the model provided a repeatable test method that can be adapted to help predict injuries to skin tissue and the performance/efficacy of personal protective equipment.
NL-EVDR: Netherlands—ExtraVascular Device Registry
Authors: Tjong Fleur V. Y. , Breeman Karel T. N. , Boersma Lucas V. A. , Yap Sing-Chien , van Erven Lieselot , van der Stoel Michelle D. , van Dijk Vincent F. , Maass Alexander H. , Knops Reinoud E. ,   NL-EVDR Working Group and NHR Device Registration Committee
Published: March 2023

AbstractCardiac implantable electronic device (CIED) therapy is an essential element in treating cardiac arrhythmias. Despite their benefits, conventional transvenous CIEDs are associated with a significant risk of mainly pocket- and lead-related complications. To overcome these complications, extravascular devices (EVDs), such as the subcutaneous implantable cardioverter-defibrillator and intracardiac leadless pacemaker, have been developed. In the near future, several other innovative EVDs will become available. However, it is difficult to evaluate EVDs in large studies because of high costs, lack of long-term follow-up, imprecise data or selected patient populations. To improve evaluation of these technologies, real-world, large-scale, long-term data are of utmost importance. A Dutch registry-based study seems to be a unique possibility for this goal due to early involvement of Dutch hospitals in novel CIEDs and an existing quality control infrastructure, the Netherlands Heart Registration (NHR). Therefore, we will soon start the Netherlands—ExtraVascular Device Registry (NL-EVDR), a Dutch nationwide registry with long-term follow-up of EVDs. The NL-EVDR will be incorporated in NHR’s device registry. Additional EVD-specific variables will be collected both retrospectively and prospectively. Hence, combining Dutch EVD data will provide highly relevant information on safety and efficacy. As a first step, a pilot project has started in selected centres in October 2022 to optimise data collection.
Samen evalueren met de doelgroep: lessen uit de ontwikkeling van een participatief evaluatie-instrument
Authors: Huiberts Irma , Pellegrom Sanne , Collard Dorine , Singh Amika , van Hartingsveldt Margo , den Broeder Lea 
Published: March 2023

SamenvattingBij het evalueren van gezondheidsbevordering is het van belang de beoogde doelgroep erbij te betrekken. In de praktijk wordt participatieve evaluatie echter nog onvoldoende ingezet. Om professionals te helpen de doelgroep bij de evaluatie te betrekken werd hiervoor in het kader van de JOGG-aanpak (Gezonde Jeugd, Gezonde Toekomst, voorheen: Jongeren Op Gezond Gewicht) een instrument ontwikkeld. Hoewel het samen met de JOGG-professionals is ontwikkeld, bleek dit instrument niet goed aan te sluiten bij hun behoeften. In dit artikel reflecteren we op hoe dit komt en delen we de geleerde lessen. Ongelijkwaardige samenwerking tussen onderzoekers en professionals heeft er aan bijgedragen dat praktijkbehoeften onvoldoende in het evaluatie-instrument zijn meegenomen. Daarnaast ervaren professionals zelf verschillende uitdagingen bij participatieve evaluatie, omdat de context waarin zij werken hen hierin onvoldoende faciliteert.