bluuebunny/crossref_metadata_2025_split huggingface dataset:
Can Sharing Improve Caring? A Call to Prioritize Shared Decision Making in Pediatric Pain Management
Authors: MacKenzie Nicole E. , Tutelman Perri R. , Chambers Christine T. , Parker Jennifer A. , Boland Laura , Lipstein Ellen A. , Jordan Isabel , Jordan Zachary , Finley G. Allen , Toupin April Karine , Witteman Holly O. , Birnie Kathryn A. 
Published: August 2023

Objective: Families and children are not consistently included in pediatric pain management decisions. Shared decision making (SDM) is a collaborative process where health care professionals (HCPs) and families exchange information about treatment options along with families’ preferences to make an optimal health decision. SDM is recommended and beneficial for children, parents, and HCPs alike; however, the process of SDM has not been routinely integrated into pediatric pain management, despite families’ desire to be involved in these decisions. In this commentary, we discuss the implications of the dearth of literature about SDM in pediatric pain on clinical practice and highlight the potential benefits of engaging in SDM to improve pain management. Method: This commentary will describe clinical approaches and considerations to implementing SDM in pediatric pain management, such as consideration of families’ preferences for SDM, the quality of the evidence, and decisional complexity. Results: This commentary will propose recommendations to further advance the understanding and utility of SDM in pediatric pain, such as identifying opportunities to engage in SDM as well as factors that support its implementation. Conclusions: SDM provides a rich and structured opportunity to engage children and families in their care, while also creating opportunities for HCPs to engage in evidence-based practice. As such, SDM should be recognized as a key priority when engaging in best practices for pediatric pain management. Implications for Impact Statement Shared decision making (SDM) facilitates the engagement of children and families in pain management decisions. SDM is not yet common practice in pediatric pain; however, it holds promise to improve pain outcomes for children by increasing application of evidence-based practices as well as satisfaction with care.
CircMIB2 therapy can effectively treat pathogenic infection by encoding a novel protein
Authors: Zheng Weiwei , Wang Linchao , Geng Shang , Yang Liyuan , Lv Xing , Xin Shiying , Xu Tianjun 
Published: August 2023

AbstractThe mRNA therapy is widely used in the treatment of diseases due to its efficient characteristics, and the COVID-19 vaccine is the application of mRNA therapy. However, due to the instability of mRNA, mRNA vaccines often need lots of modifications to ensure its stability. Recent research shows that circRNA with stable RNA structure can encode protein, which provides a new direction for mRNA therapy. Here, we discovered a novel circRNA (circMIB2) derived from E3 ubiquitin-protein ligase MIB2 (MIB2) gene in lower vertebrate fish, which can translate into a 134 amino acid protein (MIB2-134aa) through m6A modification, and is involved in innate immunity. MIB2-134aa is completely consistent with the amino acid sequence of the two domains of host gene MIB2 protein; host gene MIB2 can target TRAF6 through the two domains and inhibit the innate immune response by promoting the ubiquitination degradation of the K11-link of TRAF6, MIB2-134aa also targets TRAF6 through these same domains. Interestingly, MIB2-134aa greatly reduced the degradation of TRAF6 by its host gene MIB2. More importantly, we found that circRNA therapy of circMIB2 can significantly inhibit the colonization of Vibrio anguillarum in zebrafish, and it provides a new direction for the treatment of pathogenic diseases of fish.
A combination of a cell penetrating peptide and a protein translation inhibitor kills metastatic breast cancer cells
Authors: Rowland Linda , Marjault Henri-Baptiste , Karmi Ola , Grant DeAna , Webb Lauren J. , Friedler Assaf , Nechushtai Rachel , Elber Ron , Mittler Ron 
Published: August 2023

AbstractCell Penetrating Peptides (CPPs) are promising anticancer and antimicrobial drugs. We recently reported that a peptide derived from the human mitochondrial/ER membrane-anchored NEET protein, Nutrient Autophagy Factor 1 (NAF-1; NAF-144-67), selectively permeates and kills human metastatic epithelial breast cancer cells (MDA-MB-231), but not control epithelial cells. As cancer cells alter their phenotype during growth and metastasis, we tested whether NAF-144–67would also be efficient in killing other human epithelial breast cancer cells that may have a different phenotype. Here we report that NAF-144–67is efficient in killing BT-549, Hs 578T, MDA-MB-436, and MDA-MB-453 breast cancer cells, but that MDA-MB-157 cells are resistant to it. Upon closer examination, we found that MDA-MB-157 cells display a high content of intracellular vesicles and cellular protrusions, compared to MDA-MB-231 cells, that could protect them from NAF-144–67. Inhibiting the formation of intracellular vesicles and dynamics of cellular protrusions of MDA-MB-157 cells, using a protein translation inhibitor (the antibiotic Cycloheximide), rendered these cells highly susceptible to NAF-144–67, suggesting that under certain conditions, the killing effect of CPPs could be augmented when they are applied in combination with an antibiotic or chemotherapy agent. These findings could prove important for the treatment of metastatic cancers with CPPs and/or treatment combinations that include CPPs.
Autocrine TGF-β-positive feedback in profibrotic AT2-lineage cells plays a crucial role in non-inflammatory lung fibrogenesis
Authors: Enomoto Yasunori , Katsura Hiroaki , Fujimura Takashi , Ogata Akira , Baba Saori , Yamaoka Akira , Kihara Miho , Abe Takaya , Nishimura Osamu , Kadota Mitsutaka , Hazama Daisuke , Tanaka Yugo , Maniwa Yoshimasa , Nagano Tatsuya , Morimoto Mitsuru 
Published: August 2023

AbstractThe molecular etiology of idiopathic pulmonary fibrosis (IPF) has been extensively investigated to identify new therapeutic targets. Although anti-inflammatory treatments are not effective for patients with IPF, damaged alveolar epithelial cells play a critical role in lung fibrogenesis. Here, we establish an organoid-based lung fibrosis model using mouse and human lung tissues to assess the direct communication between damaged alveolar type II (AT2)-lineage cells and lung fibroblasts by excluding immune cells. Using this in vitro model and mouse genetics, we demonstrate that bleomycin causes DNA damage and activates p53 signaling in AT2-lineage cells, leading to AT2-to-AT1 transition-like state with a senescence-associated secretory phenotype (SASP). Among SASP-related factors, TGF-β plays an exclusive role in promoting lung fibroblast-to-myofibroblast differentiation. Moreover, the autocrine TGF-β-positive feedback loop in AT2-lineage cells is a critical cellular system in non-inflammatory lung fibrogenesis. These findings provide insights into the mechanism of IPF and potential therapeutic targets.
Portosystemic shunt placement reveals blood signatures for the development of hepatic encephalopathy through mass spectrometry
Authors: Dantas Machado Ana Carolina , Ramos Stephany Flores , Gauglitz Julia M. , Fassler Anne-Marie , Petras Daniel , Aksenov Alexander A. , Kim Un Bi , Lazarowicz Michael , Barnard Giustini Abbey , Aryafar Hamed , Vodkin Irine , Warren Curtis , Dorrestein Pieter C. , Zarrinpar Ali , Zarrinpar Amir 
Published: August 2023

AbstractElective transjugular intrahepatic portosystemic shunt (TIPS) placement can worsen cognitive dysfunction in hepatic encephalopathy (HE) patients due to toxins, including possible microbial metabolites, entering the systemic circulation. We conducted untargeted metabolomics on a prospective cohort of 22 patients with cirrhosis undergoing elective TIPS placement and followed them up to one year post TIPS for HE development. Here we suggest that pre-existing intrahepatic shunting predicts HE severity post-TIPS. Bile acid levels decrease in the peripheral vein post-TIPS, and the abundances of three specific conjugated di- and tri-hydroxylated bile acids are inversely correlated with HE grade. Bilirubins and glycerophosphocholines undergo chemical modifications pre- to post-TIPS and based on HE grade. Our results suggest that TIPS-induced metabolome changes can impact HE development, and that pre-existing intrahepatic shunting could be used to predict HE severity post-TIPS.
Purinergic signaling mediates neuroglial interactions to modulate sighs
Authors: Severs Liza J. , Bush Nicholas E. , Quina Lely A. , Hidalgo-Andrade Skyler , Burgraff Nicholas J. , Dashevskiy Tatiana , Shih Andy Y. , Baertsch Nathan A. , Ramirez Jan-Marino 
Published: August 2023

AbstractSighs prevent the collapse of alveoli in the lungs, initiate arousal under hypoxic conditions, and are an expression of sadness and relief. Sighs are periodically superimposed on normal breaths, known as eupnea. Implicated in the generation of these rhythmic behaviors is the preBötzinger complex (preBötC). Our experimental evidence suggests that purinergic signaling is necessary to generate spontaneous and hypoxia-induced sighs in a mouse model. Our results demonstrate that driving calcium increases in astrocytes through pharmacological methods robustly increases sigh, but not eupnea, frequency. Calcium imaging of preBötC slices corroborates this finding with an increase in astrocytic calcium upon application of sigh modulators, increasing intracellular calcium through g-protein signaling. Moreover, photo-activation of preBötC astrocytes is sufficient to elicit sigh activity, and this response is blocked with purinergic antagonists. We conclude that sighs are modulated through neuron-glia coupling in the preBötC network, where the distinct modulatory responses of neurons and glia allow for both rhythms to be independently regulated.
Uncovering spin-orbit coupling-independent hidden spin polarization of energy bands in antiferromagnets
Authors: Yuan Lin-Ding , Zhang Xiuwen , Acosta Carlos Mera , Zunger Alex 
Published: August 2023

AbstractMany textbook physical effects in crystals are enabled by some specific symmetries. In contrast to such ‘apparent effects’, ‘hidden effect X’ refers to the general condition where the nominal global system symmetry would disallow the effect X, whereas the symmetry of local sectors within the crystal would enable effect X. Known examples include the hidden Rashba and/or hidden Dresselhaus spin polarization that require spin-orbit coupling, but unlike their apparent counterparts are demonstrated to exist in non-magnetic systems even in inversion-symmetric crystals. Here, we discuss hidden spin polarization effect in collinear antiferromagnets without the requirement for spin-orbit coupling (SOC). Symmetry analysis suggests that antiferromagnets hosting such effect can be classified into six types depending on the global vs local symmetry. We identify which of the possible collinear antiferromagnetic compounds will harbor such hidden polarization and validate these symmetry enabling predictions with first-principles density functional calculations for several representative compounds. This will boost the theoretical and experimental efforts in finding new spin-polarized materials.
Protein engineering and iterative multimodule optimization for vitamin B6 production in Escherichia coli
Authors: Liu Linxia , Li Jinlong , Gai Yuanming , Tian Zhizhong , Wang Yanyan , Wang Tenghe , Liu Pi , Yuan Qianqian , Ma Hongwu , Lee Sang Yup , Zhang Dawei 
Published: August 2023

AbstractVitamin B6 is an essential nutrient with extensive applications in the medicine, food, animal feed, and cosmetics industries. Pyridoxine (PN), the most common commercial form of vitamin B6, is currently chemically synthesized using expensive and toxic chemicals. However, the low catalytic efficiencies of natural enzymes and the tight regulation of the metabolic pathway have hindered PN production by the microbial fermentation process. Here, we report an engineered Escherichia coli strain for PN production. Parallel pathway engineering is performed to decouple PN production and cell growth. Further, protein engineering is rationally designed including the inefficient enzymes PdxA, PdxJ, and the initial enzymes Epd and Dxs. By the iterative multimodule optimization strategy, the final strain produces 1.4 g/L of PN with productivity of 29.16 mg/L/h by fed-batch fermentation. The strategies reported here will be useful for developing microbial strains for the production of vitamins and other bioproducts having inherently low metabolic fluxes.
Birds multiplex spectral and temporal visual information via retinal On- and Off-channels
Authors: Seifert Marvin , Roberts Paul A. , Kafetzis George , Osorio Daniel , Baden Tom 
Published: August 2023

AbstractIn vertebrate vision, early retinal circuits divide incoming visual information into functionally opposite elementary signals: On and Off, transient and sustained, chromatic and achromatic. Together these signals can yield an efficient representation of the scene for transmission to the brain via the optic nerve. However, this long-standing interpretation of retinal function is based on mammals, and it is unclear whether this functional arrangement is common to all vertebrates. Here we show that male poultry chicks use a fundamentally different strategy to communicate information from the eye to the brain. Rather than using functionally opposite pairs of retinal output channels, chicks encode the polarity, timing, and spectral composition of visual stimuli in a highly correlated manner: fast achromatic information is encoded by Off-circuits, and slow chromatic information overwhelmingly by On-circuits. Moreover, most retinal output channels combine On- and Off-circuits to simultaneously encode, or multiplex, both achromatic and chromatic information. Our results from birds conform to evidence from fish, amphibians, and reptiles which retain the full ancestral complement of four spectral types of cone photoreceptors.
Toughening colloidal gels using rough building blocks
Authors: Müller Florence J. , Isa Lucio , Vermant Jan 
Published: August 2023

AbstractColloidal gels, commonly used as mesoporous intermediates or functional materials, suffer from brittleness, often showing small yield strains on the order of 1% or less for gelled colloidal suspensions. The short-range adhesive forces in most such gels are central forces—combined with the smooth morphology of particles, the resistance to yielding and shear-induced restructuring is limited. In this study, we propose an innovative approach to improve colloidal gels by introducing surface roughness to the particles to change the yield strain, giving rise to non-central interactions. To elucidate the effects of particle roughness on gel properties, we prepared thermoreversible gels made from rough or smooth silica particles using a reliable click-like-chemistry-based surface grafting technique. Rheological and optical characterization revealed that rough particle gels exhibit enhanced toughness and self-healing properties. These remarkable properties can be utilized in various applications, such as xerogel fabrication and high-fidelity extrusion 3D-printing, as we demonstrate in this study.
Stimuli-responsive rotaxane-branched dendronized polymers with tunable thermal and rheological properties
Authors: Zhu Yu , Jiang Hanqiu , Wu Weiwei , Xu Xiao-Qin , Wang Xu-Qing , Li Wei-Jian , Xu Wei-Tao , Liu GengXin , Ke Yubin , Wang Wei , Yang Hai-Bo 
Published: August 2023

AbstractAiming at the creation of polymers with attractive dynamic properties, herein, rotaxane-branched dendronized polymers (DPs) with rotaxane-branched dendrons attached onto the polymer chains are proposed. Starting from macromonomers with both rotaxane-branched dendrons and polymerization site, targeted rotaxane-branched DPs are successfully synthesized through ring-opening metathesis polymerization (ROMP). Interestingly, due to the existence of multiple switchable [2]rotaxane branches within the attached dendrons, anion-induced reversible thickness modulation of the resultant rotaxane-branched DPs is achieved, which further lead to tunable thermal and rheological properties, making them attractive platform for the construction of smart polymeric materials.
The impact of minor Cr additions in low alloy steel on corrosion behavior in simulated well environment
Authors: Gupta Kapil K. , Haratian Saber , Mishin Oleg V. , Ambat Rajan 
Published: August 2023

AbstractLow alloy steel samples with different Cr content (0‒3 wt%) have been exposed to simulated well environment. It is revealed that the 3%Cr sample initially has the highest corrosion resistance. However, due to faster formation of a FexCayCO3 protective scale in the 0%Cr sample, this sample demonstrates the highest corrosion resistance after 2 days of exposure. While the FexCayCO3 scale is also formed in the 1%Cr sample, the scale is weakly adhered and porous, which does not enable good corrosion resistance. Although the scale formation is delayed in a sample with 3 wt%Cr, once it is formed, the presence of Cr-rich phase in this scale provides greater long-term corrosion protection. Localized corrosion attack is observed in the samples with 0% Cr and 1%Cr, whereas the 3%Cr sample shows no sign of localized attack due to initial pre-passivation and the ability to rebuild the protective scale.
Profiling of repetitive RNA sequences in the blood plasma of patients with cancer
Authors: Reggiardo Roman E. , Maroli Sreelakshmi Velandi , Peddu Vikas , Davidson Andrew E. , Hill Alexander , LaMontagne Erin , Aaraj Yassmin Al , Jain Miten , Chan Stephen Y. , Kim Daniel H. 
Published: August 2023

AbstractLiquid biopsies provide a means for the profiling of cell-free RNAs secreted by cells throughout the body. Although well-annotated coding and non-coding transcripts in blood are readily detectable and can serve as biomarkers of disease, the overall diagnostic utility of the cell-free transcriptome remains unclear. Here we show that RNAs derived from transposable elements and other repeat elements are enriched in the cell-free transcriptome of patients with cancer, and that they serve as signatures for the accurate classification of the disease. We used repeat-element-aware liquid-biopsy technology and single-molecule nanopore sequencing to profile the cell-free transcriptome in plasma from patients with cancer and to examine millions of genomic features comprising all annotated genes and repeat elements throughout the genome. By aggregating individual repeat elements to the subfamily level, we found that samples with pancreatic cancer are enriched with specific Alu subfamilies, whereas other cancers have their own characteristic cell-free RNA profile. Our findings show that repetitive RNA sequences are abundant in blood and can be used as disease-specific diagnostic biomarkers.
Putting conservation gardening into practice
Authors: Munschek Marius , Witt Reinhard , Kaltofen Katrin , Segar Josiane , Wirth Christian , Weigelt Alexandra , Engelmann Rolf A. , Staude Ingmar R. 
Published: August 2023

AbstractConservation gardening (CG) represents a socio-ecological approach to address the decline of native plant species and transform the gardening industry into an innovative conservation tool. However, essential information regarding amenable plants, their ecological requirements for gardening, and commercial availability remains limited and not readily available. In this study, we present a workflow using Germany as a case study to bridge this knowledge gap. We synthesized the Red Lists of all 16 federal states in Germany, and text-mined a comprehensive platform for garden plants, as well as multiple German producers of native plants. To provide accessible information, we developed a user-friendly app (https://conservation-gardening.shinyapps.io/app-en/) that offers region-specific lists of CG plants, along with practical guidance for planting and purchasing. Our findings reveal that a median of 845 plant species are red-listed across federal states (ranging from 515 to 1123), with 41% of these species amenable to gardening (ranging from 29 to 53%), resulting in a total of 988 CG species. Notably, 66% of these species (650) are already available for purchase. Additionally, we observed that many CG plants exhibit drought tolerance and require less fertilizer on average, with implications for long-term urban planning and climate adaptation. Collaborating with gardening experts, we present a selection of purchasable CG balcony plants for each federal state, highlighting the feasibility of CG even for individuals without gardens. With a multitude of declining plants amenable to gardening and the vital role of gardens as refuges and green corridors, CG holds substantial potential to catalyze transformative change in bending the curve of biodiversity loss.
The influence of permeability and heterogeneity on chemical flooding efficiency and remaining oil distribution—based on NMR displacement imaging
Authors: Zhang Yang , Gai Changcheng , Song Binghui , Jiang Jiguo , Wang Zhiqiang 
Published: August 2023

AbstractTo investigate the impact of permeability and heterogeneity on oil displacement efficiency and remaining oil distribution of chemical flooding, three groups of high and ultrahigh permeability core samples from an ultrahigh water-cut oilfield in western China were selected as the research objects in this study. High-pressure mercury injection, scanning electron microscopy, wettability test, and other methods were used to characterize the reservoir properties of core samples. Six groups of experiments were performed using the nuclear magnetic resonance (NMR) displacement imaging technology to simulate the oilfield development process considering the economic benefits. The displacement stage with the best oil displacement effect in the process of waterflooding, chemical flooding and then waterflooding was defined, and the control effect of permeability and heterogeneity on the improvement of oil displacement efficiency by polymer-surfactant binary flooding was discussed. The distribution position of remaining oil in different displacement stages was quantitatively and visually displayed, and its control factors were revealed. The research shows that during the simulation process of first waterflooding followed by chemical flooding and then waterflooding in the oilfield, the T2 spectrum signal amplitude increases the most in the two stages, one is from saturated oil flooding to 50% water cut and the other one is from 95% water cut to the end of 1 PV polymer flooding. The oil displacement efficiency increases the most, and the oil is primarily discharged from pore throats larger than 90 ms (or with pore throat radius of 8.37 μm). Compared with heterogeneity, permeability plays a more obvious controlling role in improving the oil displacement efficiency of polymer-surfactant binary flooding. The influence of fingering phenomenon on the distribution of remaining oil is most obvious in the second waterflooding, and the distribution of remaining oil with polymer slug is more obviously affected by the fingering phenomenon than that with polymer-surfactant slug. The study results provide theoretical guidance for tapping the remaining oil potential of old oilfields with high to ultrahigh permeabilities.