bluuebunny/crossref_metadata_2025_split huggingface dataset:
Planting into a living cover crop alters preemergence herbicide dynamics and can reduce soybean yield
Authors: Nunes Jose J. , Arneson Nicholas J. , DeWerff Ryan P. , Ruark Matt , Conley Shawn , Smith Damon , Werle Rodrigo 
Published: June 2023

AbstractCereal rye cover crop (cereal rye) and preemergence (PRE) herbicides are becoming common practices for managing herbicide-resistant weeds in soybean production. Adopting these two practices in combination raises concerns regarding herbicide fate in soil, given that the cereal rye biomass can intercept the herbicide spray solution, preventing it from reaching the soil. Delaying cereal rye termination until soybean planting (planting green) optimizes biomass accumulation but might also increase PRE interception. To better understand the dynamics between cereal rye and PRE herbicides, a field experiment was conducted to evaluate two soil management practices (tillage and no-till) and two cereal rye termination practices in the planting-green system (glyphosate [1,260 g ae ha−1] and roller-crimper) on the spray deposition and fate of PRE herbicides and soybean yield. The spray deposition was assessed by placing water-sensitive paper cards on the soil surface before spraying the PRE herbicides (sulfentrazone [153 g ai ha−1] + S-metolachlor [1,379 g ai ha−1]). Herbicide concentration in soil (0 to 7.6 cm) was quantified 25 d after treatment (DAT). The presence of no-till stubble and cereal rye biomass reduced the spray coverage compared to tillage at PRE application, which reflected in a reduction in the concentration of both herbicides in soil 25 DAT. Soybean yield was reduced in all three years when the cereal rye was terminated with a roller-crimper but only reduced in one year when terminated with glyphosate. Our findings indicate that mainly cereal rye biomass reduced the concentration of PRE herbicides in the soil due to the interception of the spray solution during application. Although higher cereal rye biomass accumulation can provide better weed suppression according to the literature, farmers should be aware that the biomass can lower the concentration of PRE herbicides reaching the soil, thus intensifying field scouting to ensure that weed control is not being negatively affected.
Paleoseismology of a Major Crustal Seismogenic Source Near Mexico City: The Southern Border of the Acambay Graben
Authors: León‐Loya Rodrigo , Lacan Pierre , Ortuño María , Zúñiga F. Ramón , Štěpančíková Petra , Stemberk Jakub , Hernández Flores Ana Paula , Carrera Hernández Jaime J. , Sunyé‐Puchol Ivan , Aguirre‐Díaz Gerardo J. , Audin Laurence 
Published: June 2023

AbstractThe Trans‐Mexican Volcanic Belt is an active continental volcanic arc related to subduction along the Middle America trench. It is characterized by intra‐arc extension resulting into several major arc‐parallel active fault systems and tectonic basins. The Acambay graben, one of the largest of these basins, is located near Mexico City, in the central part of this province. In 1912, a M 6.9 earthquake ruptured the surface along the northern border of the graben together with at least two other faults. In this paper, we analyze the paleoseismic history of the southern border of the Acambay Graben, with new observations made in one natural outcrop and four paleoseismological trenches excavated across branches of the Venta de Bravo Fault at the site where it overlaps with the Pastores Fault. We present evidence of at least two paleo‐earthquakes that occurred between 12,190 ± 175 and 5,822 ± 87 cal year BP and between 647 ± 77 and 250 cal year BP. On one of these branches, we estimate a minimum slip‐rate value between 0.1 and 0.23 mm/year for the last 12 ka and a mean recurrence interval of 8.5 ± 3 ka. By considering several likely rupture lengths along the Venta de Bravo and Pastores faults, we calculated a maximum possible magnitude of Mw 7.01 ± 0.27. Finally, by correlating events recorded along different faults within the Acambay Graben, we discuss several possible rupture coalescent scenarios and related consequences for Mexico City.
Nickel Isotope Fractionation During Magmatic Differentiation
Authors: Yang Xi‐Ming , Wang Shui‐Jiong , Zhang Ya‐Wen , Dong Xu‐Han , Teng Fang‐Zhen , Helz Rosalind T. , Huang Jian , Li Xian‐Hua , Huang Shichun 
Published: June 2023

AbstractThe behavior of nickel (Ni) isotopes during magmatic differentiation is not adequately explored. Here, we find that tholeiitic rocks in the Kīlauea Iki (KI) lava lake, Hawai'i, show progressively lighter Ni isotopic compositions with increasing magmatic differentiation, whereas calc‐alkaline rocks from the thick Kamchatka arc (30–45 km), located at the convergent boundary of the Eurasian and Pacific plates show increasing Ni isotope values as MgO and Ni decrease. Forty‐three global intermediate‐felsic continental igneous rocks analyzed in this study display large Ni isotopic variations, with the Eoarchean samples having light Ni isotopic compositions that fall in the trend defined by the KI lavas, and the post‐Eoarchean samples showing systematically heavier Ni isotopic compositions overlapping those of Kamchatka arc rocks. The isotopic dichotomy results from the crystallization of isotopically heavy magnetite during low‐pressure differentiation of KI lavas, whereas the participation of sulfide separation that removes isotopically light Ni during high‐pressure differentiation of magmas traversing thick continental crust. Combined with Rhyolite‐MELTS and sulfur concentration at sulfide saturation simulations, we demonstrate that the Ni isotope fractionation during magmatic differentiation is mainly controlled by the tempo of magnetite crystallization and sulfide formation, which is a function of pressure, oxygen fugacity, and water activity. High‐pressure calc‐alkaline differentiation usually suppresses magnetite crystallization while stabilizing sulfide, leading to heavy Ni isotopic compositions for the evolved magmas, significantly deviating from the low‐pressure fractionation trend seen in the KI lavas. Ni isotopes can be used in the future as a tracer of magmatic differentiation and processes of continent formation and differentiation.
Primary cilia support cartilage regeneration after injury
Authors: Tao Dike , Zhang Lei , Ding Yunpeng , Tang Na , Xu Xiaoqiao , Li Gongchen , Niu Pingping , Yue Rui , Wang Xiaogang , Shen Yidong , Sun Yao 
Published: June 2023

AbstractIn growing children, growth plate cartilage has limited self-repair ability upon fracture injury always leading to limb growth arrest. Interestingly, one type of fracture injuries within the growth plate achieve amazing self-healing, however, the mechanism is unclear. Using this type of fracture mouse model, we discovered the activation of Hedgehog (Hh) signaling in the injured growth plate, which could activate chondrocytes in growth plate and promote cartilage repair. Primary cilia are the central transduction mediator of Hh signaling. Notably, ciliary Hh-Smo-Gli signaling pathways were enriched in the growth plate during development. Moreover, chondrocytes in resting and proliferating zone were dynamically ciliated during growth plate repair. Furthermore, conditional deletion of the ciliary core gene Ift140 in cartilage disrupted cilia-mediated Hh signaling in growth plate. More importantly, activating ciliary Hh signaling by Smoothened agonist (SAG) significantly accelerated growth plate repair after injury. In sum, primary cilia mediate Hh signaling induced the activation of stem/progenitor chondrocytes and growth plate repair after fracture injury.
Therapeutic blood-brain barrier modulation and stroke treatment by a bioengineered FZD4-selective WNT surrogate in mice
Authors: Ding Jie , Lee Sung-Jin , Vlahos Lukas , Yuki Kanako , Rada Cara C. , van Unen Vincent , Vuppalapaty Meghah , Chen Hui , Sura Asmiti , McCormick Aaron K. , Tomaske Madeline , Alwahabi Samira , Nguyen Huy , Nowatzke William , Kim Lily , Kelly Lisa , Vollrath Douglas , Califano Andrea , Yeh Wen-Chen , Li Yang , Kuo Calvin J. 
Published: June 2023

AbstractDerangements of the blood-brain barrier (BBB) or blood-retinal barrier (BRB) occur in disorders ranging from stroke, cancer, diabetic retinopathy, and Alzheimer’s disease. The Norrin/FZD4/TSPAN12 pathway activates WNT/β-catenin signaling, which is essential for BBB and BRB function. However, systemic pharmacologic FZD4 stimulation is hindered by obligate palmitoylation and insolubility of native WNTs and suboptimal properties of the FZD4-selective ligand Norrin. Here, we develop L6-F4-2, a non-lipidated, FZD4-specific surrogate which significantly improves subpicomolar affinity versus native Norrin. In Norrin knockout (NdpKO) mice, L6-F4-2 not only potently reverses neonatal retinal angiogenesis deficits, but also restores BRB and BBB function. In adult C57Bl/6J mice, post-stroke systemic delivery of L6-F4-2 strongly reduces BBB permeability, infarction, and edema, while improving neurologic score and capillary pericyte coverage. Our findings reveal systemic efficacy of a bioengineered FZD4-selective WNT surrogate during ischemic BBB dysfunction, with potential applicability to adult CNS disorders characterized by an aberrant blood-brain barrier.
Rain triggers seasonal stratification in a temperate shelf sea
Authors: Jardine J. E. , Palmer M. , Mahaffey C. , Holt J. , Wakelin S. L. , Düsterhus A. , Sharples J. , Wihsgott J. 
Published: June 2023

AbstractThe North Atlantic Storm Track acts as a conveyor belt for extratropical cyclones that frequently deliver high winds and rainfall to northwest European shelf seas. Storms are primarily considered detrimental to shelf sea stratification due to wind-driven mixing countering thermal buoyancy, but their impact on shelf scale stratification cycles remains poorly understood. Here, we show that storms trigger stratification through enhanced surface buoyancy from rainfall. A multidecadal model confirms that rainfall contributed to triggering seasonal stratification 88% of the time from 1982 to 2015. Stratification could be further modulated by large-scale climate oscillations, such as the Atlantic Multidecadal Variability (AMV), with stratification onset dates being twice as variable during a positive AMV phase than a negative one. Further insights into how changing storm activity will impact shelf seas are discussed beyond the current view of increasing wind-driven mixing, with significant implications for marine productivity and ecosystem function.
Structural insights into perilipin 3 membrane association in response to diacylglycerol accumulation
Authors: Choi Yong Mi , Ajjaji Dalila , Fleming Kaelin D. , Borbat Peter P. , Jenkins Meredith L. , Moeller Brandon E. , Fernando Shaveen , Bhatia Surita R. , Freed Jack H. , Burke John E. , Thiam Abdou Rachid , Airola Michael V. 
Published: June 2023

AbstractLipid droplets (LDs) are dynamic organelles that contain an oil core mainly composed of triglycerides (TAG) that is surrounded by a phospholipid monolayer and LD-associated proteins called perilipins (PLINs). During LD biogenesis, perilipin 3 (PLIN3) is recruited to nascent LDs as they emerge from the endoplasmic reticulum. Here, we analyze how lipid composition affects PLIN3 recruitment to membrane bilayers and LDs, and the structural changes that occur upon membrane binding. We find that the TAG precursors phosphatidic acid and diacylglycerol (DAG) recruit PLIN3 to membrane bilayers and define an expanded Perilipin-ADRP-Tip47 (PAT) domain that preferentially binds DAG-enriched membranes. Membrane binding induces a disorder to order transition of alpha helices within the PAT domain and 11-mer repeats, with intramolecular distance measurements consistent with the expanded PAT domain adopting a folded but dynamic structure upon membrane binding. In cells, PLIN3 is recruited to DAG-enriched ER membranes, and this requires both the PAT domain and 11-mer repeats. This provides molecular details of PLIN3 recruitment to nascent LDs and identifies a function of the PAT domain of PLIN3 in DAG binding.
Lipid droplets are a metabolic vulnerability in melanoma
Authors: Lumaquin-Yin Dianne , Montal Emily , Johns Eleanor , Baggiolini Arianna , Huang Ting-Hsiang , Ma Yilun , LaPlante Charlotte , Suresh Shruthy , Studer Lorenz , White Richard M. 
Published: June 2023

AbstractMelanoma exhibits numerous transcriptional cell states including neural crest-like cells as well as pigmented melanocytic cells. How these different cell states relate to distinct tumorigenic phenotypes remains unclear. Here, we use a zebrafish melanoma model to identify a transcriptional program linking the melanocytic cell state to a dependence on lipid droplets, the specialized organelle responsible for lipid storage. Single-cell RNA-sequencing of these tumors show a concordance between genes regulating pigmentation and those involved in lipid and oxidative metabolism. This state is conserved across human melanoma cell lines and patient tumors. This melanocytic state demonstrates increased fatty acid uptake, an increased number of lipid droplets, and dependence upon fatty acid oxidative metabolism. Genetic and pharmacologic suppression of lipid droplet production is sufficient to disrupt cell cycle progression and slow melanoma growth in vivo. Because the melanocytic cell state is linked to poor outcomes in patients, these data indicate a metabolic vulnerability in melanoma that depends on the lipid droplet organelle.
NOTCH4ΔL12_16 sensitizes lung adenocarcinomas to EGFR-TKIs through transcriptional down-regulation of HES1
Authors: Zhang Bin , Dong Shaowei , Wang Jian , Huang Tuxiong , Zhao Pan , Xu Jing , Liu Dongcheng , Fu Li , Wang Lingwei , Wang Guangsuo , Zou Chang 
Published: June 2023

AbstractResistance to epidermal growth factor tyrosine kinase inhibitors (EGFR-TKI) remains one of the major challenges in lung adenocarcinoma (LUAD) therapy. Here, we find an increased frequency of the L12_16 amino acid deletion mutation in the signal peptide region of NOTCH4 (NOTCH4ΔL12_16) in EGFR-TKI-sensitive patients. Functionally, exogenous induction of NOTCH4ΔL12_16 in EGFR-TKI -resistant LUAD cells sensitizes them to EGFR-TKIs. This process is mainly mediated by the reduction of the intracellular domain of NOTCH4 (NICD4) caused by the NOTCH4ΔL12_16 mutation, which results in a lower localization of NOTCH4 in the plasma membrane. Mechanistically, NICD4 transcriptionally upregulates the expression of HES1 by competitively binding to the gene promoter relative to p-STAT3. Because p-STAT3 can downregulate the expression of HES1 in EGFR-TKI-resistant LUAD cells, the reduction of NICD4 induced by NOTCH4ΔL12_16 mutation leads to a decrease in HES1. Moreover, inhibition of the NOTCH4-HES1 pathway using inhibitors and siRNAs abolishes the resistance of EGFR-TKI. Overall, we report that the NOTCH4ΔL12_16 mutation sensitizes LUAD patients to EGFR-TKIs through transcriptional down-regulation of HES1 and that targeted blockade of this signaling cohort could reverse EGFR-TKI -resistance in LUAD, providing a potential approach to overcome resistance to EGFR-TKI -therapy.
Topological quadratic-node semimetal in a photonic microring lattice
Authors: Gao Zihe , Zhao Haoqi , Wu Tianwei , Feng Xilin , Zhang Zhifeng , Qiao Xingdu , Chiu Ching-Kai , Feng Liang 
Published: June 2023

AbstractGraphene, with its two linearly dispersing Dirac points with opposite windings, is the minimal topological nodal configuration in the hexagonal Brillouin zone. Topological semimetals with higher-order nodes beyond the Dirac points have recently attracted considerable interest due to their rich chiral physics and their potential for the design of next-generation integrated devices. Here we report the experimental realization of the topological semimetal with quadratic nodes in a photonic microring lattice. Our structure hosts a robust second-order node at the center of the Brillouin zone and two Dirac points at the Brillouin zone boundary—the second minimal configuration, next to graphene, that satisfies the Nielsen–Ninomiya theorem. The symmetry-protected quadratic nodal point, together with the Dirac points, leads to the coexistence of massive and massless components in a hybrid chiral particle. This gives rise to unique transport properties, which we demonstrate by directly imaging simultaneous Klein and anti-Klein tunnelling in the microring lattice.
Fast synthesis of large-area bilayer graphene film on Cu
Authors: Zhang Jincan , Liu Xiaoting , Zhang Mengqi , Zhang Rui , Ta Huy Q. , Sun Jianbo , Wang Wendong , Zhu Wenqing , Fang Tiantian , Jia Kaicheng , Sun Xiucai , Zhang Xintong , Zhu Yeshu , Shao Jiaxin , Liu Yuchen , Gao Xin , Yang Qian , Sun Luzhao , Li Qin , Liang Fushun , Chen Heng , Zheng Liming , Wang Fuyi , Yin Wanjian , Wei Xiaoding , Yin Jianbo , Gemming Thomas , Rummeli Mark. H. , Liu Haihui , Peng Hailin , Lin Li , Liu Zhongfan 
Published: June 2023

AbstractBilayer graphene (BLG) is intriguing for its unique properties and potential applications in electronics, photonics, and mechanics. However, the chemical vapor deposition synthesis of large-area high-quality bilayer graphene on Cu is suffering from a low growth rate and limited bilayer coverage. Herein, we demonstrate the fast synthesis of meter-sized bilayer graphene film on commercial polycrystalline Cu foils by introducing trace CO2 during high-temperature growth. Continuous bilayer graphene with a high ratio of AB-stacking structure can be obtained within 20 min, which exhibits enhanced mechanical strength, uniform transmittance, and low sheet resistance in large area. Moreover, 96 and 100% AB-stacking structures were achieved in bilayer graphene grown on single-crystal Cu(111) foil and ultraflat single-crystal Cu(111)/sapphire substrates, respectively. The AB-stacking bilayer graphene exhibits tunable bandgap and performs well in photodetection. This work provides important insights into the growth mechanism and the mass production of large-area high-quality BLG on Cu.
A strong bimetal-support interaction in ethanol steam reforming
Authors: Meng Hao , Yang Yusen , Shen Tianyao , Liu Wei , Wang Lei , Yin Pan , Ren Zhen , Niu Yiming , Zhang Bingsen , Zheng Lirong , Yan Hong , Zhang Jian , Xiao Feng-Shou , Wei Min , Duan Xue 
Published: June 2023

AbstractThe metal-support interaction (MSI) in heterogeneous catalysts plays a crucial role in reforming reaction to produce renewable hydrogen, but conventional objects are limited to single metal and support. Herein, we report a type of RhNi/TiO2 catalysts with tunable RhNi-TiO2 strong bimetal-support interaction (SBMSI) derived from structure topological transformation of RhNiTi-layered double hydroxides (RhNiTi-LDHs) precursors. The resulting 0.5RhNi/TiO2 catalyst (with 0.5 wt.% Rh) exhibits extraordinary catalytic performance toward ethanol steam reforming (ESR) reaction with a H2 yield of 61.7%, a H2 production rate of 12.2 L h−1 gcat−1 and a high operational stability (300 h), which is preponderant to the state-of-the-art catalysts. By virtue of synergistic catalysis of multifunctional interface structure (Rh-Niδ−-Ov-Ti3+; Ov denotes oxygen vacancy), the generation of formate intermediate (the rate-determining step in ESR reaction) from steam reforming of CO and CHx is significantly promoted on 0.5RhNi/TiO2 catalyst, accounting for its ultra-high H2 production.
Microstructural and functional plasticity following repeated brain stimulation during cognitive training in older adults
Authors: Antonenko Daria , Fromm Anna Elisabeth , Thams Friederike , Grittner Ulrike , Meinzer Marcus , Flöel Agnes 
Published: June 2023

AbstractThe combination of repeated behavioral training with transcranial direct current stimulation (tDCS) holds promise to exert beneficial effects on brain function beyond the trained task. However, little is known about the underlying mechanisms. We performed a monocenter, single-blind randomized, placebo-controlled trial comparing cognitive training to concurrent anodal tDCS (target intervention) with cognitive training to concurrent sham tDCS (control intervention), registered at ClinicalTrial.gov (Identifier NCT03838211). The primary outcome (performance in trained task) and secondary behavioral outcomes (performance on transfer tasks) were reported elsewhere. Here, underlying mechanisms were addressed by pre-specified analyses of multimodal magnetic resonance imaging before and after a three-week executive function training with prefrontal anodal tDCS in 48 older adults. Results demonstrate that training combined with active tDCS modulated prefrontal white matter microstructure which predicted individual transfer task performance gain. Training-plus-tDCS also resulted in microstructural grey matter alterations at the stimulation site, and increased prefrontal functional connectivity. We provide insight into the mechanisms underlying neuromodulatory interventions, suggesting tDCS-induced changes in fiber organization and myelin formation, glia-related and synaptic processes in the target region, and synchronization within targeted functional networks. These findings advance the mechanistic understanding of neural tDCS effects, thereby contributing to more targeted neural network modulation in future experimental and translation tDCS applications.
Asymmetry of carbon sequestrations by plant and soil after forestation regulated by soil nitrogen
Authors: Hong Songbai , Ding Jinzhi , Kan Fei , Xu Hao , Chen Shaoyuan , Yao Yitong , Piao Shilong 
Published: June 2023

AbstractForestation is regarded as an effective strategy for increasing terrestrial carbon sequestration. However, its carbon sink potential remains uncertain due to the scarcity of large-scale sampling data and limited knowledge of the linkage between plant and soil C dynamics. Here, we conduct a large-scale survey of 163 control plots and 614 forested plots involving 25304 trees and 11700 soil samples in northern China to fill this knowledge gap. We find that forestation in northern China contributes a significant carbon sink (913.19 ± 47.58 Tg C), 74% of which is stored in biomass and 26% in soil organic carbon. Further analysis reveals that the biomass carbon sink increases initially but then decreases as soil nitrogen increases, while soil organic carbon significantly decreases in nitrogen-rich soils. These results highlight the importance of incorporating plant and soil interactions, modulated by nitrogen supply in the calculation and modelling of current and future carbon sink potential.
Two RhoGEF isoforms with distinct localisation control furrow position during asymmetric cell division
Authors: Montembault Emilie , Deduyer Irène , Claverie Marie-Charlotte , Bouit Lou , Tourasse Nicolas J. , Dupuy Denis , McCusker Derek , Royou Anne 
Published: June 2023

AbstractCytokinesis partitions cellular content between daughter cells. It relies on the formation of an acto-myosin contractile ring, whose constriction induces the ingression of the cleavage furrow between the segregated chromatids. Rho1 GTPase and its RhoGEF (Pbl) are essential for this process. However, how Rho1 is regulated to sustain furrow ingression while maintaining correct furrow position remains poorly defined. Here, we show that during asymmetric division of Drosophila neuroblasts, Rho1 is controlled by two Pbl isoforms with distinct localisation. Spindle midzone- and furrow-enriched Pbl-A focuses Rho1 at the furrow to sustain efficient ingression, while Pbl-B pan-plasma membrane localization promotes the broadening of Rho1 activity and the subsequent enrichment of myosin on the entire cortex. This enlarged zone of Rho1 activity is critical to adjust furrow position, thereby preserving correct daughter cell size asymmetry. Our work highlights how the use of isoforms with distinct localisation makes an essential process more robust.