bluuebunny/crossref_metadata_2025_split huggingface dataset:
‘Maintaining balance in life’—exploring older adults’ long-term engagement in self-managed digital fall prevention exercise
Authors: Pettersson Beatrice , Lundell Sara , Lundin-Olsson Lillemor , Sandlund Marlene 
Published: July 2023

Abstract
Background
Accidental falls are one of the greatest threats to older adults’ health and well-being. The risk of falling can be significantly reduced with strength and balance interventions. However, there needs to be further knowledge into how older adults can be supported to achieve a maintained exercise behaviour. Therefore, the aim of this study was to explore factors that enabled older adults to maintain their exercise during a 1-year self-managed digital fall prevention exercise intervention.

Methods
This study used a grounded theory methodology. Semi-structured individual interviews were conducted by phone or conference call. Eighteen community-dwelling older adults aged 70 years or more participated. The participants had a self-reported exercise dose of 60 min or more per week during the last three months of participation in a 12-months intervention of self-managed digital fall prevention exercise, the Safe Step randomized controlled trial. Open, axial, and selective coding, along with constant comparative analysis, was used to analyze the data.

Results
The analysis resulted in a theoretical model. We found that the fall prevention exercise habits of adults were developed through three stages: Acting against threats to one’s own identity, Coordinating strategies to establish a routine, and Forming habits through cues and evaluation. The main category of Maintaining balance in life encases the participants transition through the three stages and reflects balance in both physical aspects and in between activities in daily life. The process of maintaining balance in life and desire to do so were mediated both by intrinsic person-dependent factors and the Safe Step application acting as an external mediator.

Conclusion
This study identified three stages of how older adults developed self-managed fall prevention exercise habits, supported by a digital application. The generated theoretical model can inform future interventions aiming to support long-term engagement in digitally supported and self-managed fall prevention interventions.
Histone deacetylase III interactions with BK polyomavirus large tumor antigen may affect protein stability
Authors: Hsu Yueh-Han , Chao Chun-Nun , Huang Hsin-Yi , Zhao Pei-Wen , Hsu Pang-Hung , Shen Cheng-Huang , Chen San-Yuan , Fang Chiung-Yao 
Published: July 2023

Abstract
Background
Human polyomavirus BK (BKPyV) causes associated nephropathy and contributes to urinary tract cancer development in renal transplant recipients. Large tumor antigen (LT) is an early protein essential in the polyomavirus life cycle. Protein acetylation plays a critical role in regulating protein stability, so this study investigated the acetylation of the BKPyV LT protein.

Methods
The BKPyV LT nucleotide was synthesized, and the protein was expressed by transfection into permissive cells. The BKPyV LT protein was immunoprecipitated and subjected to LC-MS/MS analysis to determine the acetylation residues. The relative lysine was then mutated to arginine in the LT nucleotide and BKPyV genome to analyze the role of LT lysine acetylation in the BKPyV life cycle.

Results
BKPyV LT acetylation sites were identified at Lys3 and Lys230 by mass spectrometry. HDAC3 and HDAC8 and their deacetylation activity are required for BKPyV LT expression. In addition, mutations of Lys3 and Lys230 to arginine increased LT expression, and the interaction of HDAC3 and LT was confirmed by coimmunoprecipitation.

Conclusions
HDAC3 is a newly identified protein that interacts with BKPyV LT, and LT acetylation plays a vital role in the BKPyV life cycle.
Individualized lifestyle intervention in PCOS women (IPOS): a study protocol for a multicentric randomized controlled trial for evaluating the effectiveness of an individualized lifestyle intervention in PCOS women who wish to conceive
Authors: Malhotra Neena , Arora Taruna , Suri Vanita , Jena Saubhagya Kumar , Verma Asha , Gowri Mahasampath , Kapoor Nitin , Chalga Manjeet Singh , Kulkarni Bharati , Kamath Mohan S. 
Published: July 2023

Abstract
Background
Polycystic ovary syndrome (PCOS) is a common endocrine condition which affects women in the reproductive age group. South Asian women with PCOS have a higher risk of insulin resistance and metabolic disorder compared to women from other ethnic backgrounds. Lifestyle interventions such as dietary advice and physical exercise are recommended as a first-line management option for infertile women with PCOS. Most of the randomized controlled trials evaluating the role of lifestyle interventions in infertile PCOS women were characterized by methodological issues. The uptake of lifestyle modifications as a treatment strategy in the South Asian population is complicated by a difficult-to-change conventional high-carbohydrate diet and limited availability of space for physical activity in the region.

Methods
The study is designed as an open-label, multicentre, randomized controlled trial in South Asian women with PCOS. Women attending the fertility clinic will be screened for eligibility, and women aged between 19 and 37 years who have been diagnosed with PCOS and wishing to conceive will be invited to participate in the trial. We will include women with body mass index (BMI) between ≥ 23 and ≤ 35 kg/m2 and duration of infertility ≤ 3 years. We plan to randomize women with PCOS into two groups: group A will receive the intervention which will consist of individualized advice on diet and physical exercise along with a telephonic reminder system and follow-up visits, and group B (control) will receive one-time advice on diet and physical exercise. Both groups will receive up to three cycles of ovulation induction with letrozole after 3 months of randomization during the 6-month treatment period. The primary outcome of the trial will be the live birth following conception during the intervention period. The secondary outcomes include clinical pregnancy rate, ongoing pregnancy rate, miscarriage rate, ectopic pregnancy rate, stillbirth, time to pregnancy, mean weight loss, differences in anthropometric parameters, improvement in menstrual regularity and quality of life score.

Discussion
The IPOS trial results could help clarify and provide more robust evidence for advocating an individualized lifestyle intervention in PCOS women who wish to conceive.

Trial registration
Clinical Trial Registry of India CTRI/2023/04/051620. Registered on 13 April 2023.
Fibrinolytic and antibiotic treatment of prosthetic vascular graft infections in a novel rat model
Authors: Johansen Mikkel Illemann , Rahbek Søren Jensen , Jensen-Fangel Søren , Minero Gabriel Antonio S. , Jensen Louise Kruse , Larsen Ole Halfdan , Erikstrup Lise Tornvig , Seefeldt Anders Marthinsen , Østergaard Lars , Meyer Rikke Louise , Jørgensen Nis Pedersen 
Published: July 2023

Objectives
We developed a rat model of prosthetic vascular graft infection to assess, whether the fibrinolytic tissue plasminogen activator (tPA) could increase the efficacy of antibiotic therapy.

Materials and methods
Rats were implanted a polyethylene graft in the common carotid artery, pre-inoculated with approx. 6 log10 colony forming units (CFU) of methicillin resistant Staphylococcus aureus. Ten days after surgery, rats were randomized to either: 0.9% NaCl (n = 8), vancomycin (n = 8), vancomycin + tPA (n = 8), vancomycin + rifampicin (n = 18) or vancomycin + rifampicin + tPA (n = 18). Treatment duration was seven days. Approximately 36 hours after the end of treatment, the rats were euthanized, and grafts and organs were harvested for CFU enumeration.

Results
All animals in the control group had significantly higher CFU at the time of euthanization compared to bacterial load found on the grafts prior to inoculation (6.45 vs. 4.36 mean log10 CFU/mL, p = 0.0011), and both the procedure and infection were well tolerated.
Vancomycin and rifampicin treatment were superior to monotherapy with vancomycin, as it lead to a marked decrease in median bacterial load on the grafts (3.50 vs. 6.56 log10 CFU/mL, p = 0.0016). The addition of tPA to vancomycin and rifampicin combination treatment did not show a further decrease in bacterial load (4.078 vs. 3.50 log10 CFU/mL, p = 0.26). The cure rate was 16% in the vancomycin + rifampicin group vs. 37.5% cure rate in the vancomycin + rifampicin + tPA group. Whilst interesting, this trend was not significant at our sample size (p = 0.24).

Conclusion
We developed the first functional model of an arterial prosthetic vascular graft infection in rats. Antibiotic combination therapy with vancomycin and rifampicin was superior to vancomycin monotherapy, and the addition of tPA did not significantly reduce bacterial load, nor significantly increase cure rate.
A Randomized, Controlled Trial Comparing the Total Enteroscopy Rate and Diagnostic Efficacy of Novel Motorized Spiral Enteroscopy and Single-Balloon Enteroscopy in Patients With Small-Bowel Disorders: The Motor Trial (NCT 05548140)
Authors: Rughwani Hardik , Singh Aniruddha Pratap , Ramchandani Mohan , Jagtap Nitin , Pal Partha , Inavolu Pradev , Reddy P. Manohar , Januszewicz Wladyslaw , Sekaran Anuradha , Nabi Zaheer , Patel Rajendra , Lakhtakia Sundeep , Banerjee Rupa , Memon Sana Fathima , Balram Putta , Darishetty Santosh , Rao Guduru Venkat , Reddy D. Nageshwar 
Published: July 2023

INTRODUCTION:
Device-assisted enteroscopy has revolutionized the management of small-bowel disorders (SBD). No study to date has compared both novel motorized spiral enteroscopy (NMSE) and single-balloon enteroscopy (SBE) as a randomized controlled trial. Hence, this study was planned to include patients having SBD with the primary aim to compare the total enteroscopy rate (TER).

METHODS:
This study was conducted at the Asian Institute of Gastroenterology (AIG Hospitals), Hyderabad, India, from September 20, 2022, to December 15, 2022. All consecutive patients, older than 18 years with suspected SBD, and planned for total enteroscopy were screened for inclusion. The primary outcome was to compare the TER, and secondary outcomes were to compare the technical success, time taken to reach the depth of maximal insertion, withdrawal time, total procedure time, diagnostic yield, therapeutic success, and adverse events (AE).

RESULTS:
Seventy-two patients of the 110 patients screened were randomized in either NMSE (n = 35) or SBE (n = 37) group. The most common indication for the procedures was obscure gastrointestinal bleed (48%), others being unexplained abdominal pain with indeterminate radiologic findings (32%) and chronic diarrhea (20%). In NMSE group, the TER was 71.4%, whereas in the SBE group, it was 10.8% (P < 0.0001). The total procedure time (minutes) was much lesser with NMSE (58.17 ± 21.5 minutes) vs SBE (114.2 ± 33.5 minutes) (P < 0.0001). The diagnostic yield of NMSE (80%) was comparatively higher than SBE (62.1%) (P = 0.096). Minor AE (grade I) were observed in both the groups: NMSE 8.5% (3/35) and SBE 5.4% (2/37).

DISCUSSION:
This randomized controlled trial shows that with NMSE higher TER can be achieved in shorter duration with minimal AE, compared with SBE.
Perspectivas investigativas sobre Lectura Literaria y Mediación Lectora en Contextos Escolares
Authors: Restrepo Álvarez María Camia , Alzate Alzate Natalia Andrea , Londoño Vásquez David Alberto 
Published: July 2023

Objective: this review article is focused on the theoretical stakes that have been developed in publications from Spanish speaking countries regarding literary reading and reading mediation in school contexts, aiming to the comprehension of the topic from the relationship that it establishes with the National reading plans and the strategies to motivate the literary reading in the school. Method: for this, a qualitative bibliographic review was done from the perspective of hermeneutical analysis, with a descriptive scope. The consolidated corpus comes from research reports type publications available in Scielo, Scopus, and Ebsco, and Master and doctoral theses published in institutional repositories between 2014 and 2020. The texts found were systematized and analyzed by the means of a previously validated matrix. Conclusions and discussion: the approaches and perspectives found put the emphasis on the importance of the reading of literature in school contexts, but at the same time they draw attention to the danger of reducing the strategies and mediations only to academic and curricular aspects.
Morphology and SSR markers-based genetic diversity analysis of sesame varieties released in China
Authors: Wang Zhen , Zhou Fang , Tang Xuehui , Yang Yuanxiao , Zhou Ting , Liu Hongyan 
Published: July 2023

Abstract
Background Sesame, one of the most important oil crops in the world, is rich in sesamol, sesamin, and other antioxidant substances, and can be consumed as oil, food, and vegetable. It has a high nutritional and economic value. To reveal the genetic diversity of the sesame varieties at the molecular and morphological levels, 25 pairs of simple sequence repeat (SSR) primers and 56 biological traits were applied to 101 Chinese sesame varieties released in recent decades, as well as 62 Chinese landraces and 20 alien accessions.
Results The results indicated that a total of 166 SSR bands were detected, with an average of 6.64 per marker; the total alleles were 40.35, with an average of 1.614 per primer pair. Among them, the marker LG141 detected a maximum of 1.85 alleles, and SB393 had a minimum of 1.23 alleles. Shannon’s information index ranged from 0.2732 to 0.6497 with an average value of 0.5292; the polymorphic information index ranged from 0.0859 to 0.6357, with an average value of 0.5254, and the average heterozygosity (He) was 0.3246, suggesting that these sesame accessions have a narrow genetic diversity. The results of the unweighted pair-group method with arithmetic averaging (UPGMA) clustering were consistent with those of the binary principal component analysis, and the 183 sesame lines were categorized into three UPGMA groups. The genetic diversity coefficient of sesame germplasm is generally small, and the genetic relativeness is close. The genetic diversity coefficient of Chinese accessions is not much different from that of foreign resources. Moreover, a DNA fingerprint database of 183 sesame accessions including varieties and germplasm resources was constructed, and a corresponding two-dimensional code was developed.
Conclusion The results of this study provide data reference for the breeding and promotion of new sesame varieties in China, the protection of new variety rights, the inquiry and identification of DNA genetic information of varieties, and the development and utilization of germplasm resources domestically and abroad.
Potential Drugs in COVID-19 Management
Authors: Gasmi Amin , Noor Sadaf , Menzel Alain , Khanyk Nataliia , Semenova Yuliya , Lysiuk Roman , Beley Nataliya , Bolibrukh Liliia , Gasmi Benahmed Asma , Storchylo Olha , Bjørklund Geir 
Published: July 2023

Abstract:
The SARS-CoV-2 virus first emerged in China in December 2019 and quickly
spread worldwide. Despite the absence of a vaccination or authorized drug specifically
developed to combat this infection, certain medications recommended for other diseases
have shown potential effectiveness in treating COVID-19, although without definitive
confirmation. This review aims to evaluate the existing literature on the efficacy of these
medications against COVID-19. The review encompasses various potential treatments, including
antiviral medications, anti-malaria and anti-rheumatic drugs, vaccines, corticosteroids,
non-steroidal anti-inflammatory drugs (NSAIDs), antipyretic and analgesic
medicines, antiparasitic drugs, and statins. The analysis also addresses the potential benefits
and drawbacks of these medications, as well as their effects on hypertension and diabetes.
Although these therapies hold promise against COVID-19, further research, including
suitable product production or clinical testing, is needed to establish their therapeutic
efficacy.
Recent Updates on Peptide Molecules in Drug and Vaccine Development
Authors: Sarfaraz Mamoona , Anjum Faiqa , Zahra Duaa , Maqsood Ayesha , Ashfaq Usman Ali 
Published: July 2023

Abstract:
Peptides are of great importance in the areas of science because they can act as drug carriers and
their therapeutic effect and their ability to generate an immune response. As culturing of viral agents for drugs
and vaccine development is harmful, therefore, peptide-based drugs and vaccines have achieved great importance.
Large immunoglobulins cannot pass the plasma membrane, so peptides are used to study this interaction
because of their small size. Peptides with substituted amino acid sequences are also stable in blood serum,
which makes them significant for drug development. Peptides with substituted amino acid sequences are stable
in blood serum hence, their stability, small size, easy screening, cost-effectiveness, ease of administration and
particularity (target specificity) make them effective to be used in pharmaceutical companies. Mostly branched
peptides are used for the development of drugs because they are not prone to be degraded by proteolytic enzymes.
In peptide-based vaccines, protein acts as the main constituent from which the main component that
causes the infection is deleted by recombinant DNA technology, and these peptides act as antigens to stimulate
the immune response. Self-assembled peptides have the main role in the delivery of drugs and vaccine
molecules inside the living cells because they may also assemble into nano technological structures to improve
their efficiency. This review focuses on the characteristics of peptides that make them effective to develop
drugs and vaccines. Different peptides like synthetic peptides, antimicrobial peptides, signal peptides, carrier
peptides, and their role against various viral, pathogenic, and microbial diseases and in cosmetics are described
briefly.
Protective Effects of Liriodendrin on Myocardial Infarction-Induced
Fibrosis in Rats via the PI3K/Akt Autophagy Pathway: A Network
Pharmacology Study
Authors: Zhang Ping , Liu Xuanming , Yu Xin , Zhuo Yuzhen , Li Dihua , Yang Lei , Lu Yanmin 
Published: July 2023

Background:
Liriodendrin (LIR) has been reported to improve cardiac function in rats
following myocardial infarction. However, its role and mechanism in reparative myocardial fibrosis
remain unclear.

Methods:
In this study, a rat model of myocardial fibrosis was established via left anterior descending
artery ligation and randomly divided into three groups (n = 6 per group): sham-operated,
myocardial infarction, and LIR intervention (100 mg/kg/day) groups. The pharmacological effects
of LIR were assessed using echocardiography, hematoxylin, and eosin (H&E) staining, and Masson
staining. Network pharmacology and bioinformatics were utilized to identify potential mechanisms
of LIR, which were further validated via western blot analysis.

Results:
Our findings demonstrated that LIR improved cardiac function, histology scores, and col
lagen volume fraction. Moreover, LIR downregulated the expression of Beclin-1, LC3-II/LC3-I
while upregulating the expression of p62, indicating LIR-inhibited autophagy in the heart after
myocardial infarction. Further analysis revealed that the PI3K/Akt signaling pathway was significantly
enriched and validated by western blot. This analysis suggested that the ratios of p-
PI3K/PI3K, p-Akt/Akt, and p-mTOR/mTOR were significantly increased.

Conclusion:
LIR may attenuate myocardial infarction-induced fibrosis in rats by inhibiting excessive
myocardial autophagy, with the potential mechanism involving the activation of the
PI3K/Akt/mTOR pathway.
Impact of HLA Class I Antigen, Killer Inhibitory Receptor, and
FCGR3A Genotypes on Breast Cancer Susceptibility and Tumor
Stage
Authors: Canossi Angelica , Aureli Anna , Del Beato Tiziana , Novelli Giorgio , Buonomo Oreste , Rossi Piero , Venditti Adriano , Papola Franco , Sconocchia Giuseppe 
Published: July 2023

Background:
The identification in breast cancer (BC) of novel genetic
biomarkers regulating natural killer (NK) cell function, including the HLA, KIR, and
CD16A (FCGR3A), may be still a challenge.

Objective:
We aimed to evaluate whether the combined effect of these polymorphisms
has an impact on BC susceptibility and progression.

Methods:
47 BC Italian patients and healthy individuals (39 females and 66 males/
females) were genotyped by Sanger sequencing (HLA-C exon 2-4 and FCGR3A-
158V/F, 48L/R/H) and PCR-SSP typing (KIR genes).

Results:
HLA-C gene allele analysis showed the group C1, with HLA-C*07:02:01 allele,
to be significantly associated with tumor progression (16.7% vs. 4.0%, p=0.04,
OR=4.867), and instead, group C2, with HLA-C*05:01:01, was protective against
disease susceptibility (0.0% vs. 7.2%, p=0.019, OR=0.087). In addition, we highlighted a
significant reduction of the KIR2DS4ins in BC patients (pcorr.=0.022) and an increased
combined presence of KIR2DL1 and KIR2DS1 genes in advanced BC patients
compared to earlier stages (66.7% vs. 19.2%, p=0.002). The concurrent lack of
KIR2DL2 and KIR2DS4 genes in the presence of HLA-C2 alleles was significantly
associated with increased susceptibility to BC (p=0.012, OR=5.020) or with lymph node
involvement (p=0.008, OR=6.375). Lastly, we identified different combinations of the
FCGR3A-48/158 variants and KIR genes in BC patients compared to controls.

Conclusion:
Our findings suggest that in the development of BC probably exists a
disorder of the NK innate immunity influenced by KIR/HLA-C gene content and
FCGR3A-158 polymorphisms and that the combined analysis of these biomarkers might
help predict genetic risk scores for tailored screening of BC patients in therapy.
Faktor-Faktor yang Mempengaruhi Kinerja Keuangan Pemerintah Daerah di Kabupaten Merangin
Authors: Nurarifah Rina , Afrizal Afrizal , L Tona Aurora 
Published: July 2023

Penelitian ini merupakan studi analisis dan menjelaskan hubungan variabel Akuntabilitas, Transparansi, Sistem Pengendalian, Budaya Organisasi, Kompetensi Aparat dan Pengenolaan Keuangan Daerah yang mempengaruhi Kinerja Keuangan Pemerintah Daerah di Kabupaten Merangin. Metode yang digunakan dalam penelitian ini adalah Survey Explanatory. Kriteria pemilihan sampel adalah aparat pengelola keuangan di intansi pemerintahan di Kabupaten Merangin. Sampel penelitian ini adalah 188 responden. Analisis data dilakukan dengan menggunakan analisis statistik deskriptif berupa pencapaian kriteria indikator, dan analisis statistic inferensial menggunakan analisis kausalitas Structural Equation Modeling (SEM) berbasis component. Hasil penelitian menunjukkan bahwa Akuntabilitas tidak memiliki pengaruh positif terhadap kinerja keuangan pemerintah daerah. Transparansi tidak memiliki pengaruh positif terhadap kinerja keuangan pemerintah daerah Sistem Pengendalian internal tidak memiliki pengaruh positif terhadap kinerja keuangan pemerintah daerah. Budaya Organisasi tidak memiliki pengaruh positif terhadap kinerja keuangan pemerintah daerah. Kompetensi Aparat memiliki pengaruh positif terhadap kinerja keuangan pemerintah daerah. Pengelolaan Keuangan Daerah berpengaruh positif terhadap kinerja keuangan pemerintah daerah.