bluuebunny/crossref_metadata_2025_split huggingface dataset:
The NLRP3 activation-related signature predict the diagnosis and indicate immune characteristics in endometriosis
Authors: Nong Weihua , Wei Huimei , Dou Sheng , He Liqiao , Li Tianlong , Lin Luping , Wei Bixiao , Zhang Shun , Huo Peng , Dong Mingyou 
Published: May 2023

Abstract
Endometriosis (EMS) is a common gynecological disease leading to chronic pelvic pain and infertility in women of reproductive age, but its underlying pathogenic genes and effective treatment are still unclear. To date, abnormal expression of NLRP3 activation-related genes has been identified in EMS patients and mouse models. Therefore, this study sought to identify the key genes that could affect the diagnosis and treatment of EMS. The GSE7307 dataset was downloaded from the Gene Expression Omnibus (GEO) database, including 18 EMS samples and 23 control samples. 14 differential genes related to NLRP3 activation and EMS were obtained from the endometrial samples of GSE7307 by differential analysis. GO and KEGG analysis showed that these genes were mainly involved in the production and regulation of the cytokine IL-1β, and the NOD-like receptor signaling pathway. Random Forest (RF) and support vector machine recursive feature elimination (SVM-RFE) algorithms were used to select four diagnostic markers related to NLRP3 activation (NLRP3, IL-1β, LY96 and PDIA3) to construct the EMS diagnostic model. The four diagnostic markers were verified using western blotting and validated in the GSE7305 and GSE23339 datasets. The AUC values showed that the model had a good diagnostic performance. In addition, the infiltration of immune cells in the samples and the correlation between different immune factors and diagnostic markers were further discussed. These results suggest that four diagnostic markers may also play an important role in the immunity of EMS. Finally, 10 drugs targeting to four diagnostic markers were retrieved from the DrugBank database, of which niclosamide proved useful for treating EMS. Overall, we identified four key diagnostic genes for EMS. In addition, large-scale and multicenter prospective cohort studies are necessary to confirm whether these four genes also have valid diagnostic value in blood samples from EMS patients.
Beyond 5 GHz excitation of a ZnO-based high-overtone bulk acoustic resonator on SiC substrate
Authors: Panda Padmalochan , Chatterjee Soumyadip , Tallur Siddharth , Laha Apurba 
Published: May 2023

Abstract
This work reports on the fabrication and characterization of an Au/ZnO/Pt-based high-overtone bulk acoustic resonator (HBAR) on SiC substrates. We evaluate its microwave characteristics comparing with Si substrates for micro-electromechanical applications. Dielectric magnetron sputtering and an electron beam evaporator are employed to develop highly c-axis-oriented ZnO films and metal electrodes. The crystal structure and surface morphology of post-growth layers are characterized using X-ray diffraction (XRD), atomic force microscopy (AFM), and scanning electron microscopy (SEM) techniques. HBAR on SiC substrate results in multiple longitudinal bulk acoustic wave resonances up to 7 GHz, with the strongest excited resonances emerging at 5.25 GHz. The value of f.Q (Resonance frequency. Quality factor) parameter obtained using a novel Q approach method for HBAR on SiC substrate is 4.1 × 10 13 Hz, which to the best of our knowledge, is the highest among all reported values for specified ZnO-based devices.
Four new triterpene saponins from Cephalaria speciosa and their potent cytotoxic and immunomodulatory activities
Authors: Oztunc Ozan , Okkali Gaye Sumer , Zeinali Sevda , Nalbantsoy Ayse , Sarikahya Nazli Boke 
Published: May 2023

Abstract
Four new triterpene saponins, namely speciosides A-D (1–4) along with six known saponins were isolated from the n-butanol extract of Cephalaria speciosa. In addition to these, three new prosapogenins (1a-3a) were obtained after alkaline hydrolysis. Elucidation of the structures of the isolated compounds was carried out by 1D, 2D NMR, HR-ESI/MS and GC-MS analyses. Cytotoxic activity was investigated on A549, CCD34-Lu, MDA-MB-231, PC-3, U-87MG, HeLa, HepG-2 cells by MTT method. Additionally, the immunomodulatory effect of compounds was evaluated for macrophage polarization with/without inactivated IBV D274 antigen treatment on THP-1 cells originated macrophage cells in terms of M1 or M2. According to the cytotoxicity results, compound 1 and prosapogenin 2a exhibit significant cytotoxicity than doxorubicin by comparison. The results demonstrated that saponin molecules treated THP-1 originated macrophages were induced M1 and/or M2 polarization. Additionally, macrophage cells treated with/without IBV D274 antigen contained saponin compounds were triggered significantly M2 polarization relative to M1. Notably, monodesmosidic saponins (1 and 2a-4a) in comparison with bisdesmocidic ones (2–4) demonstrated the most effect on M2 polarization. In conclusion, the results showed that all the isolated new saponins and their prosapogenins have immunomodulatory potential on macrophage cells increasing immune response without significant cytotoxic effect on THP-1 originated macrophages.
Assessment of genetic parameters for diversified traits in periwinkle (Catharanthus roseus L.) through diallel analysis
Authors: RAVIKUMAR BOLAGAM , Dhatt K K , Jhanji Shalini 
Published: May 2023

AbstractKnowledge on complexion and level of genetic variability exist in the genotype as well the association among the different characters being indispensable for improvement in various traits. The present investigation was carried out to estimate the nature and level of genetic variability among six different inbred lines of periwinkle such as Vi-15-1, Vi-13-2, Vi-16, Vi-15-2, Vi-29 and Vi-14-3 as well as their thirty crosses combinations for ornamental characters. The highly significant variations between genotypes indicate the presence of variability in the investigated traits. The range was 5.35 to 34.41 in phenotypic coefficient of variations and 3.96 to 34.02 in genotypic coefficient of variations among the parental lines. High phenotypic and genotypic coefficient of variations among cross combinations recorded for plant spread, branches/plant and number of seeds/follicle. Moderate to high heritability together with highper centof genetic advance was recorded for plant height, spread, primary branches per plant, internode length, follicle length and seeds/follicle among the parents. Among the cross combinations plant height, plant spread, branches/plant, internode length, flower diameter, follicle length and number of seeds/follicle have shown moderate to high heritability and high genetic advance (%). It was suggested that these characters were controlled by the additive gene action and further improvement could be achieved by selection for enhancement of ornamental traits.
The Efficacy and Safety of Patiromer for Heart Failure Patients A Systematic Review and Meta-analysis
Authors: Wang  , Gao Yu , Feng Jun , Hou Linlin , Luo Chunmiao , Zhang Zhipeng 
Published: May 2023

Abstract
Purpose
To evaluate the efficacy and safety of patiromer, a novel potassium binder, in reducing the risk of hyperkalemia in patients with heart failure and optimizing the therapy of RAASi on them.
Design
Systematic review and meta-analyses.
Method
The authors conducted a systematic search in the Pubmed, Embase, Web of Science, and Cochrane Library for randomized controlled trials investigating efficacy and safety of patiromer in heart failure patients from inception to 31 January 2023 and updated on 25 March 2023. The primary outcome was the association between reduction of hyperkalemia and patiromer compared with placebo, and the secondary outcome was the association between optimization of RAASi therapy and patiromer.
Results
A total of four Randomized Controlled Trials (n=1163) were included in the study. Patiromer was found capable to reduce the risk of hyperkalemia in heart failure patients by 44% (RR 0.56, 95% CI 0.36 to 0.87; I2= 61.9%), improving tolerance to standard doses of MRA in patients with heart failure (RR 1.15, 95% CI 1.02 to 1.30; I2=49.4%), and decrease the proportion of all-cause discontinuation of RAASi (RR 0.49, 95%CI 0.25 to 0.98; I2=48.4%). However, patiromer therapy was associated with an increased risk of hypokalemia (RR 1.51, 95% CI 1.07 to 2.12; I2=0%), while no other statistically significant adverse events were observed.
Conclusion
Patiromer appears to have a considerable effect on reducing the incidence of hyperkalemia in heart failure patients and on optimizing the therapy of RAASi in those patients.
ORPHAcodes use for the coding of rare diseases: comparison of the accuracy and cross–­­country comparability
Authors: Mazzucato Monica , Pozza Laura Visonà Dalla , Facchin Paola , Angin Céline , Agius Francis , Cavero-Carbonell Clara , Corrochano Virginia , Hanusova Katerina , Kirch Kurt , Lambert Deborah , Lucano Caterina , Maiella Sylvie , Panzaru Monica , Rusu Cristina , Weber Stefanie , Zurriaga Oscar , Zvolsky Miroslav , Rath Ana 
Published: May 2023

Abstract
Background: Estimates of rare disease (RD) population impact in terms of number of affected patients and accurate disease definition is hampered by their under-representation in current coding systems. This study tested the use of a specific RD codification system (ORPHAcodes) in five European countries/regions (Czech Republic, Malta, Romania, Spain, Veneto region-Italy) across different data sources over the period January 2019-September 2021.
Results: Overall, 3,133 ORPHAcodes were used to describe RD diagnoses, mainly corresponding to the disease/subtype of disease aggregation level of the Orphanet classification (82.2%). More than half of the ORPHAcodes (53.6%) described diseases having a very low prevalence (<1 case per million), and most commonly captured rare developmental defects during embryogenesis (31.3%) and rare neurological diseases (17.6%). ORPHAcodes described disease entities more precisely than corresponding ICD-10 codes in 83.4% of cases.
Conclusions: ORPHAcodes were found to be a versatile resource for the coding of RD, able to assure consistency of use and inter-country comparability across population and hospital databases. Future research on the impact of ORPHAcoding as to the impact of numbers of RD patients with improved coding in health information systems is needed to inform on the real magnitude of this public health issue.
The investigation of dynamical behaviour variation of (2+1)-dimensional Extended Calogero-Bogoyavlenskii-Schiff Equation with different fractional operators
Authors: Mahmood Ayesha , Abbas Muhammad , Huntul M. J. , Elkenany Elkenany B. 
Published: May 2023

Abstract
The majority of scientific fields employ differential equations involving fractional-order derivatives to understanda wide range of physical events. Fractional derivatives and integrals have the ability to cop with complicatedproblems. Various types of fractional-order derivative have been dicovered, like -fractional and M-truncatedderivative. In this article the Extended Calogero-Bogoyavlenskii-Schiff Equation (CBSE) is used in its fractionalform to extract the soliton solutions, as well as to observe the behaviour of -fractional and M-truncated derivative.CBSE equation has been used to analyse various events in many fields of research. The analytical solutionsare drawn by using the Jacobi elliptic function (JEF) and the expanded tanh-coth techniques. These analyticalsolutions exhibit solitary and periodic wave behaviours. The perposed techniques are one of the effective methodsto obtain soliton solutions of partial differential equations (PDEs). The results are depicted graphically usingdifferent parametric values and the fractional parameters. The wave behaviour is observed, and shown by 3D and 2D plots.
Manifold-based Sparse Representation for Opinion Mining
Authors: Karimi Zohre 
Published: May 2023

Abstract
This paper focuses on how to enhance feature representation for opinion mining. The classical feature representation methods suffer from high dimensionality, sparsity , noisy, irrelevant and redundant information. It is proposed to exploit the manifold assumption and sparse property as prior knowledge for opinion representation to learn effective features. First, the graph representation of user reviews based on the mentioned prior knowledge is learned. Then, the spectral properties of the learned graph are exploited to present data in a new feature space. The proposed algorithm is applied to four various common input features on two benchmark datasets, Internet Movie Database (IMDB) and Amazon review dataset. Our experiments reveal that the proposed algorithm yields considerable enhancements in terms of F-measure, accuracy, and other standard performance measures compared to the combination of state-of-the-art features with various classifiers. The highest classification accuracies of 99.15 and 91.97 are obtained in the proposed method on IMDB and Amazon exploiting linear SVM classifier, respectively. The impact of parameters of the proposed algorithm is also investigated in this paper.
Will you take my (s)crap? Waste havens in the global plastic waste trade
Authors: Chunsuttiwat Pukitta , Coxhead Ian 
Published: May 2023

Abstract
For two decades until 2017, China imported more than half of the world’s traded plastic waste. Starting in 2018, however, China banned further imports of post-consumer plastic waste. The ban forced many countries to seek new ways to deal with plastic waste, including new destinations for exports. These changes may have significant impact on global social welfare and the environment. In this study we first ask whether trade in plastic waste follows a waste haven pattern, shifting environmental burden from richer countries and those with better environmental regulations to poorer countries and those with with weaker regulations. Second, we evaluate how China’s import ban altered the plastic waste trade. Empirical analysis using a gravity model reveals that the plastic waste trade follows a waste haven pattern, and the ban exacerbated this relationship. Differences in per capita GDP drove bilateral trade both before and after the ban, and disparities in stringency of environmental regulations became influential following the ban. Given that post-ban import volumes far exceeded pre-ban volumes in many countries, these results raise two concerns. First, post-ban trade increases were seemingly driven by exporters’ demand for disposal services rather than importers’ demand for plastic waste, thereby increasing environmental burden in poorer countries. Second, because countries with weak environmental regulations likely have poor waste management systems, this pattern of plastic waste redistribution may worsen the existing global plastic waste pollution crisis.|
JEL codes: F14, F18, F64, O19
Effect of treatment delay on tumor stage among symptomatic patients with earlyonset colorectal cancer: a retrospective cohort study
Authors: Sun Yaya , Jiang Guangqing , Xu Zhaiyue , Zhang Yihong , Ling Yanxuan , Shi Xin 
Published: May 2023

Abstract
Background: The incidence of colorectal cancer (CRC) among adults younger than 50 years of age has continued to increase in many countries over the past two decades. Among younger patients with CRC, a lower awareness of symptoms may contribute to treatment delay, which perhaps leads to adverse consequence on tumor stage. Therefore, the purpose of this study was to determine whether delayed treatment influences the staging of CRC among symptomatic patients under the age of 50 years.
Methods: In a retrospective cohort study, all pathologically diagnosed early-onset colorectal cancer patients (<50 years) were selected and a part of colorectal cancer patients aged 50 years and older were randomly selected by the proportion of 1:4 in Zhongda Hospital affiliated to Southeast University from March 1, 2014 to September 31, 2021. We compared clinical symptoms, pathological features, treatment delay and tumor stages between younger patients (n= 121) and older patients (n=443).
Results: Patients with early-onset CRC had higher rates of dMMR (20.0% vs 10.9% in older patients, p=0.012), high-grade adenocarcinoma (17.3% vs 9.4% in older patients, p=0.021), and mucinous adenocarcinoma (11.6% vs 5.6% in older patients, p=0.023). The median treatment delay among CRC patients was slightly longer in the younger group than in the older group (80 days [IQR 32-167] vs 53 days [IQR 20-159], p=0.028), and treatment delay was mainly caused by patient's delay. However, the proportion of stage III and stage IV tumors in younger patients (52.9%) was similar to that in older patients (46.0%) (p=0.182). In multivariable analyses, age was not a factor contributing to treatment delay in patients with CRC. Among early-onset CRC patients, 57.8% of patients with treatment delay of less than 3 months had stage III and stage IV tumors, while the proportion of stage III and stage IV tumors in patients with treatment delay of more than 3 months was 47.4%. There was no significant difference between the two groups (p=0.251).
Conclusions: In the retrospective analyses of patients with CRC in our center, we found that although treatment delay of patients with early-onset CRC was moderately longer than that of patients aged 50 years and older, age was not a factor leading to the delay in treatment. In addition, this level of delayed treatment didn't have a negative impact on tumor stage.
SARS-CoV-2 Omicron Infection in a Cohort of Hospitalized Kidney Transplant Recipients: Risk Factors of Severity
Authors: Cai Zhitao , Wang Tianyu 
Published: May 2023

Abstract
Background: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) Omicron is a major coronavirus variant, which was prevalent in China at the end of 2022 and caused widespread infection. As a low-immune group, renal transplant recipients with SARS-CoV-2 infection are prone to developing serious pneumonia or an adverse outcome event if the infection is not treated in time. Here, we analyze the possible risk factors of infection severity.
Methods: Ninety-two cases of moderate and severe SARS-CoV-2 infection after renal transplantation were collected. Statistical methods, including Fisher’s tests, F test, Spearman relative values, and multi-parameter logistic regression models, were used to analyze the risk factors for severe SARS-CoV-2 infection in renal transplant recipients.
Results: Forty-four cases complicated with hypertension wereobserved in the study cohort, among whom 30 were severe (OR: 4.63, P < 0.001). Meanwhile, 30 male patients presented with severe SARS-CoV-2 (OR: 2.45, P = 0.039) out of 51 male patients infected with Omicron. In renal transplant patients, hypertension comorbidity was closely correlated with clinical presentation (R = 0.369, P < 0.001). Interestingly, we found an increased risk of death in renal transplant patients with diabetes (OR: 5.53, P = 0.052), albeit with no significance. Blood routine test, chemistries, and additional indices showed increased neutrophils and C-reactive protein in patients with severe disease compared with themoderate group according to one-way analysis of variance (P = 0.004), while CD3 (P = 0.02) and CD4 (P = 0.04) showed lower expressional levels.Moreover, there was an evident correlation between clinical presentation and outcomes (R = 0.315, P = 0.002), which indicated that a severe status triggers worse outcomes or death events. We also observed meaningful correlations between neutrophil levels and hypertension comorbidity (R = 0.222, P = 0.034) and between interleukin-6 (IL-6) levels and diabetes comorbidity (R = 0.315, P = 0.011), with IL-6 considered a key factor in the context of coronavirus disease. Finally, urea nitrogen (P = 0.03) levels were significantly higher in patients with severe disease than in those with moderate disease, while serum creatinine (P = 0.06) levels showed no significant differences between patients with severe and moderate disease.
Conclusions: Renal transplant recipients were generally susceptible to infection with the Omicron variant, with a more pronounced incidence of severe illness observed in men than in women. Hypertension in renal transplant recipients may increase the rate of severe disease, and diabetes mellitus may further increase mortality.
HLA-identical sibling hematopoietic stem cell transplantation following reduced-toxicity myeloablative conditioning regimen in sickle cell disease
Authors: Costa Thalita , Darrigo-Junior Luiz , Grecco Carlos , Pieroni Fabiano , Faria Joana , Stracieri Ana Beatriz , Dias Juliana , Vieira Ana Carolina , Mesquita Camila , Laurindo Patrícia , Pires Bruno , Valeri Pedro , Silva Gabriela , Moraes Daniela , Loterio Lucas , Oliveira-Cardoso Érika , Santos Manoel Antônio dos , Santos Flávia , Pinto Ana Cristina , Guerino-Cunha Renato , Oliveira Maria Carolina , Traina Fabiola , Santos Antonio , De Santis Gil , Simões Belinda 
Published: May 2023

Abstract
Allogeneic hematopoietic stem cell transplantation (HSCT) is the only widely available curative treatment for sickle cell disease (SCD). Myeloablative conditioning regimens are associated with excellent outcomes in children with HLA-identical sibling donors but are limited by organ toxicity in adults. Here we report 48 children and adults who underwent HLA-identical sibling HSCT for SCD using a reduced toxicity conditioning (RTC) regimen (fludarabine, busulfan, and anti-thymocyte globulin), followed by cyclosporine plus methotrexate for graft-versus-host disease (GVHD) prophylaxis. Median (range) age at transplant and duration of follow-up were 16.5 (7–35) years and 77.5 (1-169) months, respectively. Indication for HSCT included neurological complications in 25 (52.1%) patients and 10 (20.8%) were alloimmunized against red blood cell antigens. All patients achieved engraftment, except one who died before engraftment period. Secondary graft failure, grade ≥ 2 acute GHVD and chronic GVHD were present in 7 (14.6%), 10 (20.8%) and 7 (14.6%) patients, respectively. Five-year overall survival (OS) and event-free survival (EFS) (95% CI) were 91% (77.8–96.5) and 80.3% (65.5–89.2), respectively. Survival curves were not different between children and adults (p = 0.37 and p = 0.33, respectively). RTC regimen is safe and effective, with acceptable toxicity and incidence of GVHD, in children and adults with SCD.