bluuebunny/crossref_metadata_2025_split huggingface dataset:
Investigation of a Dissipating Mesospheric Bore Using Airglow Imager and Direct Numerical Simulation
Authors: Ramachandran Kesava , Sivakandan Mani , Chau Jorge L. , Urco Juan M. , Gerding Michael , Grundmann Sven , Smith Steven M. 
Published: April 2023

AbstractAtmospheric gravity waves play an important role in driving the dynamics of the Mesosphere and Lower Thermosphere and the basic structure of this region is determined by momentum deposition of these waves. Mesospheric bores are a type of non‐linear response that cause the amplification of gravity wave, due to trapping, that is characterized by a propagating step‐like jump followed by undulating waves. They require a stable layer or duct to travel horizontally with little attenuation thereby capable of transporting wave energy and momentum over larger distances. We present a prominent bright undular bore event observed in the mesospheric O(1S), O2, and OH emission layers on 16 March 2021 over Germany. A striking feature of this observation is the capture of bore's rapid dissipation around the center of the imager's field of view. The vertical temperature profile obtained from the satellite data indicates the presence of temperature inversion layer which acted as a thermal duct for the bore propagation. In addition, we have performed idealized two dimensional direct numerical simulations (DNS) of Navier‐Stokes equations under Boussinesq approximation. The DNS results reproduce many important characteristics of the observed airglow event like the nonlinear wave‐steepening, number of trailing waves, and its dissipation by implementing a thermal duct and a wave‐like perturbation. Furthermore, the DNS results also indicate that the duct width and amplitude of the initial perturbation have a considerable effect on the bore morphology.
Intranasal booster using an Omicron vaccine confers broad mucosal and systemic immunity against SARS-CoV-2 variants
Authors: Wang Qian , Yang Chenchen , Yin Li , Sun Jing , Wang Wei , Li Hengchun , Zhang Zhengyuan , Chen Si , Liu Bo , Liu Zijian , Shi Linjing , Liu Xiaolin , Guan Suhua , Wang Chunhua , Qu Linbing , Feng Ying , Niu Xuefeng , Feng Liqiang , Zhao Jincun , Li Pingchao , Chen Ling , Zhong Nanshan 
Published: April 2023

AbstractThe highly contagious SARS-CoV-2 Omicron subvariants severely attenuated the effectiveness of currently licensed SARS-CoV-2 vaccines based on ancestral strains administered via intramuscular injection. In this study, we generated a recombinant, replication-incompetent human adenovirus type 5, Ad5-S-Omicron, that expresses Omicron BA.1 spike. Intranasal, but not intramuscular vaccination, elicited spike-specific respiratory mucosal IgA and residential T cell immune responses, in addition to systemic neutralizing antibodies and T cell immune responses against most Omicron subvariants. We tested intranasal Ad5-S-Omicron as a heterologous booster in mice that previously received intramuscular injection of inactivated ancestral vaccine. In addition to inducing serum broadly neutralizing antibodies, there was a significant induction of respiratory mucosal IgA and neutralizing activities against Omicron subvariants BA.1, BA.2, BA.5, BA.2.75, BF.7 as well as pre-Omicron strains Wildtype, Beta, and Delta. Serum and mucosal neutralizing activities against recently emerged XBB, BQ.1, and BQ.1.1 could also be detected but were much lower. Nasal lavage fluids from intranasal vaccination contained multimeric IgA that can bind to at least 10 spike proteins, including Omicron subvariants and pre-Omicron strains, and possessed broadly neutralizing activities. Intranasal vaccination using Ad5-S-Omicron or instillation of intranasal vaccinee’s nasal lavage fluids in mouse nostrils protected mice against Omicron challenge. Taken together, intranasal Ad5-S-Omicron booster on the basis of ancestral vaccines can establish effective mucosal and systemic immunity against Omicron subvariants and multiple SARS-CoV-2 variants. This candidate vaccine warrants further development as a safe, effective, and user-friendly infection and transmission-blocking vaccine.
A ferritin-based COVID-19 nanoparticle vaccine that elicits robust, durable, broad-spectrum neutralizing antisera in non-human primates
Authors: Weidenbacher Payton A.-B. , Sanyal Mrinmoy , Friedland Natalia , Tang Shaogeng , Arunachalam Prabhu S. , Hu Mengyun , Kumru Ozan S. , Morris Mary Kate , Fontenot Jane , Shirreff Lisa , Do Jonathan , Cheng Ya-Chen , Vasudevan Gayathri , Feinberg Mark B. , Villinger Francois J. , Hanson Carl , Joshi Sangeeta B. , Volkin David B. , Pulendran Bali , Kim Peter S. 
Published: April 2023

AbstractWhile the rapid development of COVID-19 vaccines has been a scientific triumph, the need remains for a globally available vaccine that provides longer-lasting immunity against present and future SARS-CoV-2 variants of concern (VOCs). Here, we describe DCFHP, a ferritin-based, protein-nanoparticle vaccine candidate that, when formulated with aluminum hydroxide as the sole adjuvant (DCFHP-alum), elicits potent and durable neutralizing antisera in non-human primates against known VOCs, including Omicron BQ.1, as well as against SARS-CoV-1. Following a booster ~one year after the initial immunization, DCFHP-alum elicits a robust anamnestic response. To enable global accessibility, we generated a cell line that can enable production of thousands of vaccine doses per liter of cell culture and show that DCFHP-alum maintains potency for at least 14 days at temperatures exceeding standard room temperature. DCFHP-alum has potential as a once-yearly (or less frequent) booster vaccine, and as a primary vaccine for pediatric use including in infants.
Temperate functional niche availability not resident-invader competition shapes tropicalisation in reef fishes
Authors: Miller Mark G. R. , Reimer James D. , Sommer Brigitte , Cook Katie M. , Pandolfi John M. , Obuchi Masami , Beger Maria 
Published: April 2023

AbstractTemperate reefs are at the forefront of warming-induced community alterations resulting from poleward range shifts. This tropicalisation is exemplified and amplified by tropical species’ invasions of temperate herbivory functions. However, whether other temperate ecosystem functions are similarly invaded by tropical species, and by what drivers, remains unclear. We examine tropicalisation footprints in nine reef fish functional groups using trait-based analyses and biomass of 550 fish species across tropical to temperate gradients in Japan and Australia. We discover that functional niches in transitional communities are asynchronously invaded by tropical species, but with congruent invasion schedules for functional groups across the two hemispheres. These differences in functional group tropicalisation point to habitat availability as a key determinant of multi-species range shifts, as in the majority of functional groups tropical and temperate species share functional niche space in suitable habitat. Competition among species from different thermal guilds played little part in limiting tropicalisation, rather available functional space occupied by temperate species indicates that tropical species can invade. Characterising these drivers of reef tropicalisation is pivotal to understanding, predicting, and managing marine community transformation.
Structure and mechanism of the alkane-oxidizing enzyme AlkB
Authors: Guo Xue , Zhang Jianxiu , Han Lei , Lee Juliet , Williams Shoshana C. , Forsberg Allison , Xu Yan , Austin Rachel Narehood , Feng Liang 
Published: April 2023

AbstractAlkanes are the most energy-rich form of carbon and are widely dispersed in the environment. Their transformation by microbes represents a key step in the global carbon cycle. Alkane monooxygenase (AlkB), a membrane-spanning metalloenzyme, converts straight chain alkanes to alcohols in the first step of the microbially-mediated degradation of alkanes, thereby playing a critical role in the global cycling of carbon and the bioremediation of oil. AlkB biodiversity is attributed to its ability to oxidize alkanes of various chain lengths, while individual AlkBs target a relatively narrow range. Mechanisms of substrate selectivity and catalytic activity remain elusive. Here we report the cryo-EM structure of AlkB, which provides a distinct architecture for membrane enzymes. Our structure and functional studies reveal an unexpected diiron center configuration and identify molecular determinants for substrate selectivity. These findings provide insight into the catalytic mechanism of AlkB and shed light on its function in alkane-degrading microorganisms.
Phosphorylation and stabilization of EZH2 by DCAF1/VprBP trigger aberrant gene silencing in colon cancer
Authors: Ghate Nikhil B. , Kim Sungmin , Shin Yonghwan , Kim Jinman , Doche Michael , Valena Scott , Situ Alan , Kim Sangnam , Rhie Suhn K. , Lenz Heinz-Josef , Ulmer Tobias S. , Mumenthaler Shannon M. , An Woojin 
Published: April 2023

AbstractOur recent work has shown that DCAF1 (also known as VprBP) is overexpressed in colon cancer and phosphorylates histone H2AT120 to drive epigenetic gene inactivation and oncogenic transformation. We have extended these observations by investigating whether DCAF1 also phosphorylates non-histone proteins as an additional mechanism linking its kinase activity to colon cancer development. We now demonstrate that DCAF1 phosphorylates EZH2 at T367 to augment its nuclear stabilization and enzymatic activity in colon cancer cells. Consistent with this mechanistic role, DCAF1-mediated EZH2 phosphorylation leads to elevated levels of H3K27me3 and altered expression of growth regulatory genes in cancer cells. Furthermore, our preclinical studies using organoid and xenograft models revealed that EZH2 requires phosphorylation for its oncogenic function, which may have therapeutic implications for gene reactivation in colon cancer cells. Together, our data define a mechanism underlying DCAF1-driven colonic tumorigenesis by linking DCAF1-mediated EZH2 phosphorylation to EZH2 stability that is crucial for establishing H3K27me3 and gene silencing program.
Inactivated vaccine-elicited potent antibodies can broadly neutralize SARS-CoV-2 circulating variants
Authors: Liu Yubin , Wang Ziyi , Zhuang Xinyu , Zhang Shengnan , Chen Zhicheng , Zou Yan , Sheng Jie , Li Tianpeng , Tai Wanbo , Yu Jinfang , Wang Yanqun , Zhang Zhaoyong , Chen Yunfeng , Tong Liangqin , Yu Xi , Wu Linjuan , Chen Dong , Zhang Renli , Jin Ningyi , Shen Weijun , Zhao Jincun , Tian Mingyao , Wang Xinquan , Cheng Gong 
Published: April 2023

AbstractA full understanding of the inactivated COVID-19 vaccine-mediated antibody responses to SARS-CoV-2 circulating variants will inform vaccine effectiveness and vaccination development strategies. Here, we offer insights into the inactivated vaccine-induced antibody responses after prime-boost vaccination at both the polyclonal and monoclonal levels. We characterized the VDJ sequence of 118 monoclonal antibodies (mAbs) and found that 20 neutralizing mAbs showed varied potency and breadth against a range of variants including XBB.1.5, BQ.1.1, and BN.1. Bispecific antibodies (bsAbs) based on nonoverlapping mAbs exhibited enhanced neutralizing potency and breadth against the most antibody-evasive strains, such as XBB.1.5, BQ.1.1, and BN.1. The passive transfer of mAbs or their bsAb effectively protected female hACE2 transgenic mice from challenge with an infectious Delta or Omicron BA.2 variant. The neutralization mechanisms of these antibodies were determined by structural characterization. Overall, a broad spectrum of potent and distinct neutralizing antibodies can be induced in individuals immunized with the SARS-CoV-2 inactivated vaccine BBIBP-CorV, suggesting the application potential of inactivated vaccines and these antibodies for preventing infection by SARS-CoV-2 circulating variants.
Copper(I)-catalyzed asymmetric alkylation of α-imino-esters
Authors: Liu Zong-Ci , Wang Zi-Qing , Zhang Xuan , Yin Liang 
Published: April 2023

AbstractAsymmetric alkylation of enolates is one of the most direct and important reactions to prepare α-chiral carbonyl compounds. Except for the classical methods that rely on the use of chiral auxiliaries, asymmetric catalysis emerged as a powerful tool, especially asymmetric phase-transfer catalysis. However, in the field of transition metal catalysis, only limited success with asymmetric alkylation of enolates was achieved. Hereby, we disclose a copper(I)-catalyzed asymmetric alkylation of α-imino-esters with various alkyl halides, including allyl bromides, propargyl bromide, benzyl bromides, α-bromo carbonyl compounds, and alkyl iodides. Both linear and cyclic α-imino-esters serve as competent pronucleophiles in the alkylation, which affords α-amino acid derivatives bearing either a trisubstituted or a tetrasubstituted stereogenic carbon center in high to excellent enantioselectivity. Control experiments indicate that the α-imino-ester is activated by a chiral copper(I)-phosphine complex through coordination, thus enabling facile deprotonation to provide a stabilized copper(I)-enolate in the presence of a mild base. Finally, the mildly basic nature allows the asymmetric alkylation of chiral dipeptides with excellent both chemo- and enantioselectivities.
The median time to stopover exclusive breastfeeding among employed and unemployed mothers of infants aged 6–12 months in Ethiopia, 2019
Authors: Adugnaw Emebet , Gizaw Getu , Girma Merom , Arage Getachew , Libanos Mebratu , Emrie Wondosen Addis , Chanie Sewnet Sisay , Chanie Ermias Sisay 
Published: April 2023

AbstractEarly discontinuation of breastfeeding is known to be associated with avoidable childhood morbidity and mortality. The effect of maternal employment on the duration of exclusive breastfeeding and its determinants has not been addressed adequately in in Ethiopia in general and in the stud area in particular. Hence, this study was aimed to compare the time to stop exclusive breastfeeding and its determinants among employed and unemployed mothers of infants 6–12 months of age. A community-based comparative cross-sectional study was conducted from March 1 to 30, 2019. A total of 426 mothers were recruited using a simple random sampling technique. The Kaplan–Meier curve with log-rank test was used to compare the difference in cessation of exclusive breastfeeding before 6 months. Bivariate and Cox proportional hazards model were computed. Hazard ratios and their 95% confidence intervals were computed to determine the level of significance. Four hundred twenty-six (213 employed and 213 un-employed) mothers were included in the final analysis. The median duration of exclusive breastfeeding was 4 months and 6 months for infants of employed and unemployed mothers, respectively. The likelihood of ceasing of exclusive breastfeeding before 6 months of age was significantly associated with family support of exclusive breastfeeding [AHR = 3.99, 95% CI (1.9, 8.3)], and lack of exclusive breastfeeding counseling during postnatal care [AHR = 7.76, 95% CI (2.99, 20.1)], primipara mothers [AHR = 1.5, 95% CI (1.14, 2.04)], maternity leave of 4 months [AHR = 7, 95% CI 2.2, 22.2)] and employed mothers [AHR = 3.77, 95% CI (2.4, 5.9)]. The median duration of exclusive breastfeeding was shorter among employed mothers than un-employed mothers. It is clear from this study that cessation of exclusive breastfeeding was associated with the duration of paid maternity leave for employed mothers. Family support and perceived adequacy of breast milk were associated with cessation of exclusive breastfeeding before 6 months among unemployed mothers.
First-principles calculations of equilibrium Ga isotope fractionations between several important Ga-bearing minerals and aqueous solutions
Authors: Zhang Jixi 
Published: April 2023

AbstractThis study predicts the equilibrium isotope fractionation factors for some important Ga-bearing species, including major minerals, aqueous solutions and gas phase systems. Equilibrium isotope fractionations of Ga were investigated by using the first-principles quantum chemistry method at the B3LYP/6-311+G(d) level. The 103ln(RPFR) values of orthoclase, albite, quartz, kaolinite, forsterite, montmorillonite, gibbsite, cassiterite, aragonite, sphalerite and calcite were calculated with the volume variable cluster model. The 103ln(RPFR)s of these minerals decrease in the following order: orthoclase > albite > quartz > kaolinite > forsterite > montmorillonite > gibbsite > cassiterite > aragonite > sphalerite > calcite. The solvation effect of Ga3+-bearing aqueous species is modeled by the water-droplet method, and the 103ln(RPFR)s of Ga3+-bearing aqueous species decrease in the following order: [Ga(OH)4]− > [Ga(OH)3] > [Ga(OH)]2+ > [Ga(OH)2]+ > [Ga(H2O)6]3+. The calculation results show that equilibrium isotope fractionations of Ga between different minerals, solutions and gas phases are appreciable. Among minerals, Ga isotope fractionation exhibits the largest value between orthoclase and calcite. Ga isotopic fractionation factor between these two minerals can reach 3.18 per mil at 100 °C. Ga isotope fractionations between Ga-bearing aqueous species and minerals are important for obtaining information about the different geochemical processes, such as surficial geochemistry. This study has provided important Ga isotope fractionation factors.
Effectiveness of emotion regulation strategies measured by self-report and EMG as a result of strategy used, negative emotion strength and participants’ baseline HRV
Authors: Kobylińska Dorota , Lewczuk Karol , Wizła Magdalena , Marcowski Przemysław , Blaison Christophe , Kastendieck Till , Hess Ursula 
Published: April 2023

AbstractWe investigated how emotion regulation (ER) effectiveness—on both a self-reported rating as well as emotional expression (corrugator supercilii muscle activity) level—is affected by the characteristics of the situation (low vs. high negativity), the strategy used (reinterpretation, distraction, suppression, no regulation control condition) and individual dispositions (low vs. high baseline Heart Rate Variability) as well as their interaction. For this purpose, 54 adult women participated in a laboratory study. All the included factors significantly influenced both corrugator activity and appraisals of pictures’ negativity (in specific experimental conditions). For example, for high HRV participants, (1) distraction, suppression and reinterpretation significantly decreased corrugator activity compared to the control condition, and (2) distraction decreased appraised picture negativity for high negativity photos. For low HRV participants, distraction and suppression were most effective in decreasing corrugator responses, while suppression was more effective than reinterpretation in decreasing perceived picture negativity in the high negativity condition. Subjectively reported effort and success in applying ER strategies were also dependent on manipulated and dispositional factors. Overall, our results lend support to the flexible emotion regulation framework, showing that emotion regulation effectiveness relies on situational context as well as individual dispositions and their interaction.
Chronic Mycobacterium avium infection differentially affects the cytokine expression profile of three mouse strains, but has no effect on behavior
Authors: Roque Susana , de Sá-Calçada Daniela , Cerqueira-Rodrigues Bruno , Monteiro Susana , Guerreiro Susana G. , Palha Joana A. , Correia-Neves Margarida 
Published: April 2023

AbstractOne of the most remarkable findings in the immunology and neuroscience fields was the discovery of the bidirectional interaction between the immune and the central nervous systems. This interplay is tightly regulated to maintain homeostasis in physiological conditions. Disruption in this interplay has been suggested to be associated with several neuropsychiatric disorders. Most studies addressing the impact of an immune system disruption on behavioral alterations focus on acute pro-inflammatory responses. However, chronic infections are highly prevalent and associated with an altered cytokine milieu that persists over time. Studies addressing the potential effect of mycobacterial infections on mood behavior originated discordant results and this relationship needs to be further addressed. To increase our understanding on the effect of chronic infections on the central nervous system, we evaluated the role of Mycobacterium avium infection. A model of peripheral chronic infection with M. avium in female from three mouse strains (Balb/c, C57BL/6, and CD-1) was used. The effect of the infection was evaluated in the cytokine expression profile (spleen and hippocampus), hippocampal cell proliferation, neuronal plasticity, serum corticosterone production and mood behavior. The results show that M. avium peripheral chronic infection induces alterations not just in the peripheral immune system but also in the central nervous system, namely in the hippocampus. Interestingly, the cytokine expression profile alterations vary between mouse strains, and are not accompanied by hippocampal cell proliferation or neuronal plasticity changes. Accordingly, no differences were observed in locomotor, anxious and depressive-like behaviors, in any of the mouse strains used. We conclude that the M. avium 2447 infection-induced alterations in the cytokine expression profile, both in the periphery and the hippocampus, are insufficient to alter hippocampal plasticity and behavior.
Interrelationships between urban travel demand and electricity consumption: a deep learning approach
Authors: Movahedi Ali , Parsa Amir Bahador , Rozhkov Anton , Lee Dongwoo , Mohammadian Abolfazl Kouros , Derrible Sybil 
Published: April 2023

AbstractThe analysis of infrastructure use data in relation to other components of the infrastructure can help better understand the interrelationships between infrastructures to eventually enhance their sustainability and resilience. In this study, we focus on electricity consumption and travel demand. In short, the premise is that when people are in buildings consuming electricity, they are not generating traffic on roads, and vice versa, hence the presence of interrelationships. We use Long Short Term Memory (LSTM) networks to model electricity consumption patterns of zip codes based on the traffic volume of the same zip code and nearby zip codes. For this, we merge two datasets for November 2017 in Chicago: (1) aggregated electricity use data in 30-min intervals within the city of Chicago and (2) traffic volume data captured on the Chicago expressway network. Four analyses are conducted to identify interrelationships: (a) correlation between two time series, (b) temporal relationships, (c) spatial relationships, and (d) prediction of electricity consumption based on the total traffic volume. Overall, from over 250 models, we identify and discuss complex interrelationships between travel demand and electricity consumption. We also analyze and discuss how and why model performance varies across Chicago.