bluuebunny/crossref_metadata_2025_split huggingface dataset:
Czego uczy dyskusja wokół anglosaskich komisji etycznych? Analiza wybranych kwestii spornych
Authors: Surmiak Adrianna 
Published: June 2023

W krajach anglosaskich komisje etyczne mają długą historię i silną pozycję, chociaż jednocześnie ich funkcjonowanie wywołuje dyskusje w środowisku badaczy społecznych. W artykule analizuję wybrane kwestie sporne, które koncentrują się wokół trzech pytań: 1) Kogo chronią i powinny chronić komisje etyczne? 2) Czy komisje etyczne powinny tak samo chronić wszystkich uczestników badań? 3) Kiedy komisje etyczne mogą ingerować w metodologię ocenianego projektu badawczego? Przyjrzenie się tym sporom jest istotne, gdyż może pomóc w lepszym funkcjonowaniu komisji etycznych dla badań społecznych w Polsce. W ostatniej dekadzie ich liczba znacząco wzrosła, jednak nie towarzyszył temu szerszy namysł ani nad ich działaniem, ani nad wątpliwościami związanymi z taką kontrolą.
FBXO5 acts as a novel prognostic biomarker for patients with cervical cancer
Authors: Jiang Shan , Zheng Jianfeng , Cui Zhaolei , Li Yanhong , Wu Qiaoling , Cai Xintong , Zheng Chaoqiang , Sun Yang 
Published: June 2023

Background: Cervical cancer (CC) remains one of the most common and deadly malignancies in women worldwide. FBXO5, a protein-coding gene, is highly expressed in a variety of primary tumors and promotes tumor progression, however, its role and prognostic value in CC remain largely unknown.Methods: A key differential gene, FBXO5, was screened according to WGCNA based on immunohistochemical assays of clinical samples, multiple analyses of the Cancer Genome Atlas (TCGA) and Genotype-Tissue Expression (GTEx) databases, including survival analysis, tumor mutational burden, GO, KEGG, tumor immune infiltration, and chemotherapeutic drug sensitivity, to explore the expression and prognostic value of FBXO5 in CC. The migration and invasiveness of cervical cancer cells following FBXO5 knockdown and overexpression were examined using wound healing and transwell assays, and the viability of cancer cells was assessed using CCK8 and EdU assays.Results:FBXO5 was discovered to be substantially expressed in CC tissues using data from our CC cohort and the TCGA database, and a survival analysis indicated FBXO5 as a predictive factor for poor overall survival in CC patients. In vitro, CC cells were more inclined to proliferate, migrate, and invade when FBXO5 was upregulated as opposed to when it was knocked down.
Using multiple indicators to predict the risk of surgical site infection after ORIF of tibia fractures: a machine learning based study
Authors: Ying Hui , Guo Bo-Wen , Wu Hai-Jian , Zhu Rong-Ping , Liu Wen-Cai , Zhong Hong-Fa 
Published: June 2023

ObjectiveSurgical site infection (SSI) are a serious complication that can occur after open reduction and internal fixation (ORIF) of tibial fractures, leading to severe consequences. This study aimed to develop a machine learning (ML)-based predictive model to screen high-risk patients of SSI following ORIF of tibial fractures, thereby aiding in personalized prevention and treatment.MethodsPatients who underwent ORIF of tibial fractures between January 2018 and October 2022 at the Department of Emergency Trauma Surgery at Ganzhou People’s Hospital were retrospectively included. The demographic characteristics, surgery-related variables and laboratory indicators of patients were collected in the inpatient electronic medical records. Ten different machine learning algorithms were employed to develop the prediction model, and the performance of the models was evaluated to select the best predictive model. Ten-fold cross validation for the training set and ROC curves for the test set were used to evaluate model performance. The decision curve and calibration curve analysis were used to verify the clinical value of the model, and the relative importance of features in the model was analyzed.ResultsA total of 351 patients who underwent ORIF of tibia fractures were included in this study, among whom 51 (14.53%) had SSI and 300 (85.47%) did not. Of the patients with SSI, 15 cases were of deep infection, and 36 cases were of superficial infection. Given the initial parameters, the ET, LR and RF are the top three algorithms with excellent performance. Ten-fold cross-validation on the training set and ROC curves on the test set revealed that the ET model had the best performance, with AUC values of 0.853 and 0.866, respectively. The decision curve analysis and calibration curves also showed that the ET model had the best clinical utility. Finally, the performance of the ET model was further tested, and the relative importance of features in the model was analyzed.ConclusionIn this study, we constructed a multivariate prediction model for SSI after ORIF of tibial fracture through ML, and the strength of this study was the use of multiple indicators to establish an infection prediction model, which can better reflect the real situation of patients, and the model show great clinical prediction performance.
Exploring PANoptosis in breast cancer based on scRNA-seq and bulk-seq
Authors: He Puxing , Ma Yixuan , Wu Yaolu , Zhou Qing , Du Huan 
Published: June 2023

BackgroundPANoptosis, a cell death pathway involving pyroptosis, apoptosis, and necroptosis, is pivotal in the development of malignancy. However, in the field of breast cancer, the interaction between PANoptosis and tumor cells has not been thoroughly explored.MethodsWe downloaded breast cancer data and GSE176078 single-cell sequencing dataset from Gene Expression Omnibus (GEO) and The Cancer Genome Atlas (TCGA) databases to obtain PANoptosis-associated genes. To construct prognostic models, COX and LASSO regression was used to identify PANoptosis-associated genes with prognostic value. Finally, immune infiltration analysis and differential analysis of biological functions were performed.ResultsRisk grouping was performed according to the prognostic model constructed by COX regression and LASSO regression. The low-risk group showed a better prognosis (P &lt; 0.05) and possessed higher levels of immune infiltration and expression of immune checkpoint-related genes. In addition, the lower the risk score, the higher the degree of microsatellite instability (MSI). Meanwhile, radixin (RDX), the gene with the highest hazard ratio (HR) value among PANoptosis prognosis-related genes, was explicitly expressed in artery Iendothelial cells (ECs) and was widely involved in signaling pathways such as immune response and cell proliferation, possessing rich biological functions.ConclusionWe demonstrated the potential of PANoptosis-based molecular clustering and prognostic features in predicting the survival of breast cancer patients. Furthermore, this study has led to a deeper understanding of the role of PANoptosis in breast cancer and has the potential to provide new directions for immunotherapy of breast cancer.
Effects of meiotic stage-specific oocyte vitrification on mouse oocyte quality and developmental competence
Authors: Deng Dongmei , Xie Juan , Tian Yin , Zhu Ling , Liu Xuemei , Liu Junxia , Huang Guoning , Li Jingyu 
Published: June 2023

IntroductionAcquisition of germinal vesicle (GV) stage oocytes for fertility preservation (FP) offers several benefits over in vivo matured oocyte cryopreservation following ovarian stimulation, particularly for cancer patients necessitating immediate treatment. Two FP approaches for GV oocytes are available: vitrification before in vitro maturation (IVM) at the GV stage (GV-VI) or post-IVM at the metaphase II (MII) stage (MII-VI). The optimal method remains to be determined.MethodsIn this study, mouse oocytes were collected without hormonal stimulation and vitrified either at the GV stage or the MII stage following IVM; non-vitrified in vitro matured MII oocytes served as the control (CON). The oocyte quality and developmental competence were assessed to obtain a better method for immediate FP.ResultsNo significant differences in IVM and survival rates were observed among the three groups. Nevertheless, GV-VI oocytes exhibited inferior quality, including abnormal spindle arrangement, mitochondrial dysfunction, and early apoptosis, compared to MII-VI and CON oocytes. Oocyte vitrification at the GV stage impacted maternal mRNA degradation during IVM. In addition, the GV-VI group demonstrated significantly lower embryonic developmental competence relative to the MII-VI group. RNA sequencing of 2-cell stage embryos revealed abnormal minor zygotic genome activation in the GV-VI group.ConclusionVitrification at the GV stage compromised oocyte quality and reduced developmental competence. Consequently, compared to the GV stage, oocyte vitrification at the MII stage after IVM is more suitable for patients who require immediate FP.
Food anticipatory behaviour on European seabass in sea cages: activity-, positioning-, and density-based approaches
Authors: Chen I-Hao , Georgopoulou Dimitra G. , Ebbesson Lars O. E. , Voskakis Dimitris , Lal Pradeep , Papandroulakis Nikos 
Published: June 2023

IntroductionFarmed fish like European seabass (Dicentrarchus labrax) anticipate meals if these are provided at one or multiple fixed times during the day. The increase in locomotor activity is typically known as food anticipatory activity (FAA) and can be observed several hours prior to feeding. Measuring FAA is often done by demand feeders or external sensors such as cameras or light curtains. However, purely locomotor-activity-based FAA may provide an incomplete view of feeding and prefeeding behaviour.MethodsHere, we show that FAA can be measured through passive acoustic telemetry utilising three different approaches and suggest that adding more means to food anticipation detection is beneficial. We compared the diving behaviour, acceleration activity, and temperature of 22 tagged individuals over the period of 12 days and observed FAA through locomotor activity, depth position, and density-based unsupervised clustering (i.e., DBSCAN).ResultsOur results demonstrate that the position- and density-based methods also provide expressions of anticipatory behaviour that can be interchangeable with locomotor-driven FAA or precede it.DiscussionWe, therefore, support a unified framework for food anticipation: FAA should only describe locomotor-driven FAA. Food anticipatory positioning (FAP) should be a term for position-based (P-FAP) and density-based (D-FAP) methods for food anticipation. Lastly, FAP, together with the newly defined FAA, should become part of an umbrella term that is already in use: food anticipatory behaviour (FAB). Our work provides data-driven approaches to each FAB category and compares them with each other. Furthermore, accurate FAB windows through FAA and FAP can help increase fish welfare in the aquaculture industry, and the more approaches available, the more flexible and more robust the usage of FAB for a holistic view can be achieved.
High-sensitivity cardiac SPECT system design with collimator-less interspaced mosaic-patterned scintillators
Authors: Wang Rui , Zhang Debin , Hu Yifan , Lyu Zhenlei , Ma Tianyu 
Published: June 2023

PurposeSingle-photon emission computed tomography (SPECT) is an important tool for myocardial perfusion imaging (MPI). Mechanical collimators cause the resolution-sensitivity trade-off in the existing cardiac SPECT systems, which hinders fast cardiac scan capability. In this work, we propose a novel collimator-less cardiac SPECT system with interspaced mosaic-patterned scintillators, aiming to significantly improve sensitivity and reduce scan time without trading-off image resolution.MethodsWe propose to assemble a collimator-less cardiac SPECT with 7 mosaic-patterned detector modules forming a half-ring geometry. The detector module consists of 10 blocks, each of which is assembled with 768 sparsely distributed scintillators with a size of 1.68 mm × 1.68 mm × 20 mm, forming a mosaic pattern in the trans-axial direction. Each scintillator bar contains 5 GAGG(Ce) scintillators and 5 optical-guide elements, forming a mosaic pattern in the axial direction. In the Monte Carlo simulations, the in-plane resolution and axial resolution are evaluated using a hot-rod phantom and 5 disk phantoms, respectively. We simulate a cardiac phantom that is placed in a water-filled cylinder and evaluate the image performance with different data acquisition time. We perform image reconstruction with the expectation–maximization algorithm using system matrices derived from the simulation of a uniform cylindrical source filling the field-of-view (FOV). Besides, a 2-D prototype system is designed to demonstrate the feasibility of the collimator-less imaging concept.ResultsIn the simulation system, the sensitivity is 16.31% ± 8.85% in a 180 mm (Φ) × 100  mm (L) FOV. The 6-mm rods in the hot rod phantom and the 5-mm disks in the disk phantom are clearly separable. Satisfactory MPI image quality is achieved in the cardiac phantom study with an acquisition time of 30 s. In prototype experiments, the point sources with an 8 mm center-to-center distance are clearly separable at different positions across the FOV.ConclusionThe study reveals a promising approach to high-sensitivity SPECT imaging without a heavy-metal collimator. In cardiac imaging, this approach opens the way to a very fast cardiac scan with good resolution. Further works are ongoing to build a practical 3-D imaging system based on the existing design.
The risk factors and impact of subchorionic hematoma in the first trimester in IVF twin pregnancies: a prospective cohort study
Authors: Mei Youwen , Lin Yonghong , Guo Xiaoli , Zhang Yangping , Wang Fang 
Published: June 2023

ObjectiveThis study aimed to identify the risk factors for subchorionic hematoma (SCH) in the first trimester of in vitro fertilization (IVF) twin pregnancies and investigate the impact of SCH on pregnancy outcomes.Study designA prospective cohort study was conducted at Chengdu Women and Children's Central Hospital. The study recruited patients who were identified with twin pregnancies in the first trimester, undergoing IVF treatment from January 2020 to May 2021. The demographic characteristics and pregnancy outcomes were compared between the SCH and the non-SCH groups. A logistic regression analysis was used to determine the risk factors for SCH and adverse pregnancy outcomes.ResultsIn the first trimester, 38% of patients developed SCH. The independent risk factors for SCH included male factor, hydrosalpinx, polycystic ovary syndrome (PCOS), previous miscarriage, and adenomyosis. With respect to the pregnancy outcomes, only the rate of twin pregnancy loss before 20 gestational weeks was significantly higher in the SCH group than in the non-SCH group. After adjusting for the confounding factors, the presence of SCH diminished the ovarian reserve, and previous miscarriage was independently related to twin pregnancy loss before 20 gestational weeks.ConclusionThis may be the first study to evaluate the risk factors of SCH in twin pregnancies who underwent IVF-ET/FET treatment, which may provide some theoretical basis for clinical practice in the future. Furthermore, it was found that the occurrence of SCH was associated with the loss of both pregnancies before 20 gestational weeks. Therefore, these patients should be offered increased surveillance and timely treatment.
What defines a great surgeon? A survey study confronting perspectives
Authors: Luscan Romain , Malheiro Emeline , Sisso Fiona , Wartelle Sébastien , Parc Yann , Fauroux Brigitte , Bégué Thierry , Johanet Hubert , Denoyelle Françoise , Garabédian Erea-Noël , Simon François 
Published: June 2023

BackgroundThe definition of a great surgeon is usually reported by surgeons themselves. The objective of the study was to define a multifaceted definition of a great surgeon, by confronting patients', healthcare workers', and surgeons' perspectives.Study designAn online open-ended questionnaire was created to identify three qualities and three shortcomings defining a great surgeon. Age, gender, and profession of respondents were collected. Responses with a similar meaning were combined into word groups and labeled within four themes: human qualities, technical surgical skills (TSS), non-technical skills (NTS), and knowledge. Multivariate analyses were conducted between themes and respondent characteristics.ResultsFour thousand seven hundred and sixty qualities and 4,374 shortcomings were obtained from 1,620 respondents including 385 surgeons, 291 patients, 565 operating theater (OT) health professionals, and 379 non-OT health professionals. The main three qualities were dexterity (54% of respondents), meticulousness (18%), and empathy (18%). There was no significant difference between professional categories for TSS. Compared with surgeons, non-OT health professionals and patients put more emphasis on human qualities (29 vs. 39% and 42%, respectively, p &lt; .001). OT health professionals referred more to NTS than surgeons (35 vs. 22%, p &lt; 0.001). Knowledge was more important for surgeons (19%) than for all other professional categories (p &lt; 0.001).ConclusionsThis survey illustrates the multifaceted definition of a great surgeon. Even if dexterity is a major quality, human qualities are of paramount importance. Knowledge seems to be underestimated by non-surgeons, although it essential to understand the disease and preparing the patient and OT team for the procedure.
Wood fibers are a crucial microhabitat for cellulose- and xylan- degrading bacteria in the hindgut of the wood-feeding beetle Odontotaenius disjunctus
Authors: Schwarz Melbert , Beza-Beza Cristian F. , Mikaelyan Aram 
Published: June 2023

IntroductionWood digestion in insects relies on the maintenance of a mosaic of numerous microhabitats, each colonized by distinct microbiomes. Understanding the division of digestive labor between these microhabitats- is central to understanding the physiology and evolution of symbiotic wood digestion. A microhabitat that has emerged to be of direct relevance to the process of lignocellulose digestion is the surface of ingested plant material. Wood particles in the guts of some termites are colonized by a specialized bacterial fiber-digesting microbiome, but whether this represents a widespread strategy among insect lineages that have independently evolved wood-feeding remains an open question.MethodsIn this study, we investigated the bacterial communities specifically associated with wood fibers in the gut of the passalid beetle Odontotaenius disjunctus. We developed a Percoll-based centrifugation method to isolate and enrich the wood particles from the anterior hindgut, allowing us to access the wood fibers and their associated microbiome. We then performed assays of enzyme activity and used short-read and long-read amplicon sequencing of the 16S rRNA gene to identify the composition of the fiber-associated microbiome.ResultsOur assays demonstrated that the anterior hindgut, which houses a majority of the bacterial load, is an important site for lignocellulose digestion. Wood particles enriched from the anterior hindgut contribute to a large proportion of the total enzyme activity. The sequencing revealed that O. disjunctus, like termites, harbors a distinct fiber-associated microbiome, but notably, its community is enriched in insect-specific groups of Lactococcus and Turicibacter.DiscussionOur study underscores the importance of microhabitats in fostering the complex symbiotic relationships between wood-feeding insects and their microbiomes. The discovery of distinct fiber-digesting symbionts in O. disjunctus, compared to termites, highlights the diverse evolutionary paths insects have taken to adapt to a challenging diet.
Hypovirus infection induces proliferation and perturbs functions of mitochondria in the chestnut blight fungus
Authors: Wang Jinzi , Quan Rui , He Xipu , Fu Qiang , Tian Shigen , Zhao Lijiu , Li Shuangcai , Shi Liming , Li Ru , Chen Baoshan 
Published: June 2023

IntroductionThe chestnut blight fungus, Cryphonectria parasitica, and hypovirus have been used as a model to probe the mechanism of virulence and regulation of traits important to the host fungus. Previous studies have indicated that mitochondria could be the primary target of the hypovirus.MethodsIn this study, we report a comprehensive and comparative study comprising mitochondrion quantification, reactive oxygen species (ROS) and respiratory efficiency, and quantitative mitochondrial proteomics of the wild-type and virus-infected strains of the chestnut blight fungus.Results and discussionOur data show that hypovirus infection increases the total number of mitochondria, lowers the general ROS level, and increases mitochondrial respiratory efficiency. Quantification of mitochondrial proteomes revealed that a set of proteins functioning in energy metabolism and mitochondrial morphogenesis, as well as virulence, were regulated by the virus. In addition, two viral proteins, p29 and p48, were found to co-fractionate with the mitochondrial membrane and matrix. These results suggest that hypovirus perturbs the host mitochondrial functions to result in hypovirulence.
Factors associated with stroke after COVID-19 vaccination: a statewide analysis
Authors: Nahab Fadi , Bayakly Rana , Sexton Mary Elizabeth , Lemuel-Clarke Manet , Henriquez Laura , Rangaraju Srikant , Ido Moges 
Published: June 2023

BackgroundThe objective of our study was to evaluate vaccine type, COVID-19 infection, and their association with stroke soon after COVID-19 vaccination.MethodsIn a retrospective cohort study, we estimated the 21-day post-vaccination incidence of stroke among the recipients of the first dose of a COVID-19 vaccine. We linked the Georgia Immunization Registry with the Georgia Coverdell Acute Stroke Registry and the Georgia State Electronic Notifiable Disease Surveillance System data to assess the relative risk of stroke by the vaccine type.ResultsApproximately 5 million adult Georgians received at least one COVID-19 vaccine between 1 December 2020 and 28 February 2022: 54% received BNT162b2, 41% received mRNA-1273, and 5% received Ad26.COV2.S. Those with concurrent COVID-19 infection within 21 days post-vaccination had an increased risk of ischemic (OR = 8.00, 95% CI: 4.18, 15.31) and hemorrhagic stroke (OR = 5.23, 95% CI: 1.11, 24.64) with no evidence for interaction between the vaccine type and concurrent COVID-19 infection. The 21-day post-vaccination incidence of ischemic stroke was 8.14, 11.14, and 10.48 per 100,000 for BNT162b2, mRNA-1273, and Ad26.COV2.S recipients, respectively. After adjusting for age, race, gender, and COVID-19 infection status, there was a 57% higher risk (OR = 1.57, 95% CI: 1.02, 2.42) for ischemic stroke within 21 days of vaccination associated with the Ad26.COV2.S vaccine compared to BNT162b2; there was no difference in stroke risk between mRNA-1273 and BNT162b2.ConclusionConcurrent COVID-19 infection had the strongest association with early ischemic and hemorrhagic stroke after the first dose of COVID-19 vaccination. Although not all determinants of stroke, particularly comorbidities, were considered in this analysis, the Ad26.COV2.S vaccine was associated with a higher risk of early post-vaccination ischemic stroke than BNT162b2.