bluuebunny/crossref_metadata_2025_split huggingface dataset:
Multi-label transcriptional classification of colorectal cancer reflects tumor cell population heterogeneity
Authors: Cascianelli Silvia , Barbera Chiara , Ulla Alexandra Ambra , Grassi Elena , Lupo Barbara , Pasini Diego , Bertotti Andrea , Trusolino Livio , Medico Enzo , Isella Claudio , Masseroli Marco 
Published: May 2023

Abstract
Background
Transcriptional classification has been used to stratify colorectal cancer (CRC) into molecular subtypes with distinct biological and clinical features. However, it is not clear whether such subtypes represent discrete, mutually exclusive entities or molecular/phenotypic states with potential overlap. Therefore, we focused on the CRC Intrinsic Subtype (CRIS) classifier and evaluated whether assigning multiple CRIS subtypes to the same sample provides additional clinically and biologically relevant information.

Methods
A multi-label version of the CRIS classifier (multiCRIS) was applied to newly generated RNA-seq profiles from 606 CRC patient-derived xenografts (PDXs), together with human CRC bulk and single-cell RNA-seq datasets. Biological and clinical associations of single- and multi-label CRIS were compared. Finally, a machine learning-based multi-label CRIS predictor (ML2CRIS) was developed for single-sample classification.

Results
Surprisingly, about half of the CRC cases could be significantly assigned to more than one CRIS subtype. Single-cell RNA-seq analysis revealed that multiple CRIS membership can be a consequence of the concomitant presence of cells of different CRIS class or, less frequently, of cells with hybrid phenotype. Multi-label assignments were found to improve prediction of CRC prognosis and response to treatment. Finally, the ML2CRIS classifier was validated for retaining the same biological and clinical associations also in the context of single-sample classification.

Conclusions
These results show that CRIS subtypes retain their biological and clinical features even when concomitantly assigned to the same CRC sample. This approach could be potentially extended to other cancer types and classification systems.
Integrable aspects, analytic solutions and their asymptotic analysis for a discrete relativistic Toda lattice system
Authors: Qin Meng-Li , Wen Xiao-Yong 
Published: May 2023

AbstractIn this paper, we investigate a discrete relativistic Toda lattice ($\mathrm{dRTL}_{+}(\alpha )$

dRTL
+

(
α
)
) system, which may describe particle vibrations in lattices with an exponential interaction force. First, we construct its discrete generalized $(m,2N-m)$
(
m
,
2
N
−
m
)
-fold Darboux transformation, from which we can explicitly give its analytic solutions, such as discrete multi-soliton solutions, position controllable rational and semi-rational solutions and their hyperbolic-and-rational mixed solutions, whose properties and dynamics are analyzed and shown graphically. Second, the asymptotic behaviors of diverse exact solutions are analyzed, which shows that the interactions among different solutions are always elastic. In particular, the position of controllable rational solutions and asymptotic state analysis of discrete hyperbolic-and-rational mixed solutions are obtained and discussed for the first time. Finally, we study some integrable properties of this system, such as the integrable hierarchy and relevant Hamiltonian structures and conservation laws from a discrete spectral problem. These results may be helpful for understanding nonlinear lattice dynamics.
COVID-19 pandemic response in the Meuse-Rhine Euroregion: methods, participation and recommendations of a longitudinal cross-border study
Authors: Stabourlos C. , van Bilsen C. J. A. , Brinkhues S. , Moonen C. P. B. , Demarest S. , Hanssen D. A. T. , van Loo I. H. M. , Savelkoul P. H. M. , Philippsen D. , van der Zanden B. A. M. , Dukers-Muijrers N. H. T. M. , Hoebe C. J. P. A. 
Published: May 2023

Abstract
Background
Comparative data collection in transborder areas can contribute to informed decision making processes when dealing with borderless health threats such as pandemics, and thus help minimize the negative health effects for its citizens. To examine the pandemic response over time and the impact of infectious disease control in a cross-border setting, a prospective longitudinal study was conducted in the border area between Germany, Belgium and the Netherlands. In the spring of 2021, a random sample of 26,925 adult citizens selected from governmental registries was invited to collect a blood sample at home for SARS-CoV-2 antibody testing and to fill in an online questionnaire on attitudes and behaviour towards infection prevention measures, cross-border mobility, social network and support, COVID-19 self-reported infection(s) and symptoms, vaccination, general self-reported health and socio-demographics. In autumn 2021, participants were invited for a follow-up round. An online tool was developed to coordinate fieldwork procedures, real-time monitoring of participation and consultation of antibody test results. Furthermore, a helpdesk in all three languages for participants’ support was set up.

Results
In the first round, 6,006 citizens in the Meuse-Rhine Euroregion participated. 15.3% of the invited citizens on the Belgian side of the border participated. In the Netherlands and Germany this was respectively 27% and 23.7%. In the follow-up round 4,286 (71.4%) citizens participated for the second time. The participation rate was highest in the age group 50–69 years and lowest in > 80 in all sub regions of the Meuse-Rhine Euroregion. More women participated than men. Overall, more blood samples were returned than completed questionnaires. In total, 3,344 citizens in the Meuse-Rhine Euroregion completed all components of participation in both rounds.

Conclusions
The collection of comparative data can help better assess the pandemic response and the impact of infectious disease control in a cross-border area. Recommendations for a longitudinal cross-border study include a centralized online environment, mapping out potential challenges related to national regulations in the preparation phase and organizing regional coordination centres to create more familiarity and trust towards the involved organisations.
FVC/DLCO identifies pulmonary hypertension and predicts 5-year all-cause mortality in patients with COPD
Authors: Li Yuer , Zhang Rui , Shan Hu , Shi Wenhua , Feng Xiaoli , Chen Haijuan , Yang Xia , Li Yali , Zhang Jie , Zhang Ming 
Published: May 2023

Abstract
Background
Pulmonary hypertension (PH) is a common complication of chronic obstructive pulmonary disease (COPD). However, it is unknown whether the ratio of forced vital capacity (FVC) to diffusing lung capacity for carbon monoxide (DLCO) can identify PH in the patients with COPD and predict its prognosis.

Methods
The study population I included 937 COPD patients who were admitted to inpatient treatments from 2010 to 2017, and finally 750 patients were available to follow-up the 5-year all-cause mortality (study population II). Clinical characteristics of the study population were recorded.

Results
COPD patients with PH had a higher FVC/DLCO value compared with the patients without PH. The threshold for FVC/DLCO to identify PH in COPD patients was 0.44 l/mmol/min/kPa. Multivariate logistic regression analysis showed that FVC/DLCO was a significant predictor for PH in the patients with COPD. The study population II showed that the 5-year all-cause mortality of COPD patients was significantly higher in combined with PH group than without PH group. Compared with the survivor group, FVC/DLCO value was significantly increased in non-survivor group. The threshold for FVC/DLCO to predict 5-year all-cause mortality was 0.41 l/mmol/min/kPa. Kaplan–Meier survival curves showed that 5-year cumulative survival rate for COPD patients were significantly decreased when the value of FVC/DLCO was ≥ 0.41 l/mmol/min/kPa. Multivariate cox regression analysis showed that FVC/DLCO was an independent prognostic factor for 5-year all-cause mortality in COPD patients.

Conclusion
FVC/DLCO could identify PH in the patients with COPD and was an independent predictor for 5-year all-cause mortality of COPD.
Association between corrected serum calcium levels after dialysis and post-dialysis fatigue risk: a hospital-based case–control study
Authors: Xiao Ping , Wang Zhi-Hong , Lu Yan , Zhang Shuang , Jin Yu-Xin , Liu Xin , Jiang Zhen-Li , Liu Shu-Xin 
Published: May 2023

Abstract
Objective
Post-dialysis fatigue (PDF) is an important problem in patients undergoing maintenance hemodialysis (MHD); however, evidence of the association between serum calcium after dialysis and the risk of PDF is limited and controversial. We explored this association among patients receiving MHD.

Methods
We carried out a case–control study of patients in the dialysis unit of Dalian Municipal Central Hospital between December 2019 and January 2020, including 340 patients with PDF and 270 patients without PDF. PDF was assessed by a \validated self-administered questionnaire. Clinical variables were tested for multicollinearity using variance inflation factor analysis. Corrected serum calcium levels were categorized into three groups, with the lowest tertile used as the reference category. The odds ratios (ORs) and corresponding 95% confidence intervals (CIs) for PDF risk were estimated using an unconditional logistic regression model.

Result
After adjusting for potential confounders, corrected serum calcium levels showed a significant positive association with the risk of PDF (ORT3vs.T1 = 1.61, 95% CI = 1.01–2.58). Notably, after stratification by age, corrected serum calcium was also positively associated with the risk of PDF in patients aged ≥ 65 years (ORT3vs.T1  = 4.25, 95% CI 1.66–11.46). Furthermore, a significant linear trend and interaction were also observed (P < 0.05).

Discussion
Higher corrected serum calcium levels after dialysis might increase the risk of PDF among MHD patients. However, further studies are warranted to confirm these findings.
Disordered eating and considerations for the transgender community: a review of the literature and clinical guidance for assessment and treatment
Authors: McGregor Kerry , McKenna John L. , Barrera Ellis P. , Williams Coleen R. , Hartman-Munick Sydney M. , Guss Carly E. 
Published: May 2023

Abstract
Background
It has been well established that individuals who identify as lesbian, gay, bisexual, transgender, and queer are at increased risk for mental health pathology, including eating disorders/disordered eating behaviors (ED/DEB). However, less is understood about the unique experiences of transgender and gender diverse (TGD) people who struggle with ED/DEB.

Aims
The purpose of this literature review is to examine the literature regarding the unique risk factors for TGD individuals who experience ED/DEB through a lens informed by the minority stress model. Additionally, guidance around the assessment and clinical management of eating disorders for TGD individuals will be presented.

Results
TGD people are at increased risk for developing ED/DEB due to a number of factors including: gender dysphoria, minority stress, the desire to pass, and barriers to gender affirming care.

Conclusion
While guidance around assessment and treatment of ED/DEB for TGD individuals is still limited, adhering to a gender affirmative care model is essential.
Age similarities in the anchoring effect in emotion intensity judgment
Authors: Jin Menghan , Peng Huamao , Wang Dahua 
Published: May 2023

Abstract
Background
The anchoring effect refers to the tendency that an individual’s numerical judgment would assimilate to an anchor (a numerical value) that appears before that judgment. This study investigated whether the anchoring effect exists in the emotion judgment of younger and older adults and observed the age-related characteristics. This could not only broaden the explanation of the anchoring effect but also link this classic judgment bias with daily emotion judgment to refresh our understanding of older adults’ ability in emotional perspective taking.

Method
Participants (older adults: n = 64, age range: 60–74, 27 males; younger adults: n = 68, age range: 18–34, 34 males) read a brief emotional story and compared the protagonist’s emotion intensity to a given numerical anchor (lower or higher than the anchor) and then estimated the protagonist’s possible emotion intensity in that story. The task was divided into two cases according to anchor relevance (anchors are relevant or irrelevant relative to the judgment target).

Results
The results showed that the estimates were higher under high-anchor than low-anchor conditions, suggesting the robust anchoring effect. Further, the anchoring effect was greater for anchor-relevant than anchor-irrelevant tasks and for negative rather than positive emotions. No age differences were found.

Discussion and conclusions
The results indicated that the anchoring effect is robust and stable for younger and older adults, even though the anchor information seemed irrelevant. Finally, perceiving others’ negative emotions is a crucial but rather difficult aspect of empathy, which could be a challenge and requires more caution for accurate interpretation.
Cleaning testing of nineteenth-century plaster surface models with thin polyacrylamide-based gel layers attached to flexible polyethylene films
Authors: Theodorakopoulos Charis , Risdonne Valentina , Freese Silvia , Diraoui Samar , Jonas Ulrich 
Published: May 2023

Abstract
This paper explores the cleaning efficacy of polyethylene-supported 15-min photo-crosslinked poly (acrylamide-co-benzophenone) surface-attached gels (SAGs) on gypsum plaster surface models inspired by nineteenth-century casts. Cleaning tests were performed on plaster surface models with and without organic and inorganic coatings, which had been exposed to accelerated ageing by heat, humidity, and light after artificial soiling. The specific types of SAG systems were selected based on their water loading and dehydration capacities. The SAGs were loaded with customized solutions and applied on the plaster models for one minute. Cleaning efficacy was evaluated with visible reflectance, UV fluorescence photography, scanning electron microscopy, colorimetry, UV/Vis spectrophotometry, glossimetry, high resolution 3D microscopy, attenuated total reflection Fourier transform infrared spectroscopy and 2D Fourier transform infrared imaging. The SAGs provided fast and minimal wetting of the substrates, prevented excessive liquid spreading and allowed the effective liquid contact with the soiled gypsum plaster surface. A striking removal of soils from the gypsum plaster surface models was observed, which suggests convenient application of the SAG systems for the cleaning of historical plaster objects.
Parameter identification and range restriction through sensitivity analysis for a high-temperature heat injection test
Authors: Heldt Stefan , Wang Bo , Bauer Sebastian 
Published: May 2023

AbstractIn order to compensate for the variable mismatch between heat demand and heat production from renewable sources or waste heat, high-temperature aquifer thermal energy storage (HT-ATES) is a promising option. A reliable prediction of the energetic performance as well as thermal and hydraulic impacts of a HT-ATES requires a suitable model parameterization regarding the subsurface properties. In order to identify the subsurface parameters on which investigation efforts should be focused, we carried out an extensive sensitivity analysis of the thermal and hydraulic parameters for a high-temperature heat injection test (HIT) using numerical modeling of the governing coupled thermo-hydraulic processes. The heat injection test was carried out in a quaternary shallow aquifer using injection temperatures of about 75 °C over 5 days, accompanied by an extensive temperature monitoring. The sensitivity analysis is conducted for parameter ranges based on literature values, based on site investigation at the HIT site and based on a model calibrated to the measured temperature distribution following the heat injection. Comparing the parameter ranges thus obtained in this three-step approach allows to identify those parameters, for which model prediction uncertainty decreased most, which are also the parameters, that strongly affect the thermal behavior. The highest sensitivity is found for vertical and horizontal hydraulic conductivity as well as for groundwater flow velocity, indicating that investigation efforts for HT-ATES projects should focus on these parameters. Heat capacity and thermal conductivity have a smaller impact on the temperature distribution. Our work thus yields a consistent approach to identifying the parameters which can be best restricted by field investigations and subsequent model calibration. Focusing on these during field investigations thus enable improved model predictions of both HT-ATES operation and induced impacts.
Effects of combinational mycelia obtained via in vitro culture on ageing and reproduction in Drosophila melanogaster and their mechanisms
Authors: Huang Yaqin , Li Jinzhe , Wang Dezhi , Zhu Nailiang , Qiao Xinrong 
Published: May 2023

AbstractThis research serves to investigate the effects of mycelium combinations obtained by in vitro culture of Amanita virgineoides Bas, Phallus rubicundus (Bosc) Fr., and Lepista nuda (Bull.) Cooke fruiting bodies on ageing and reproduction of Drosophila melanogaster and their underlying mechanisms. The ageing effect was most pronounced in the A. virgineoides + P. rubicundus combination group, with a maximum mortality rate of 18.33% at 6 h. The catalase (CAT) and superoxide dismutase (SOD) activities, as well as the transcripts of the longevity genes Nrf2 and Hep, were significantly downregulated to minima at 9 and 18 h, respectively. The malondialdehyde (MDA) content and transcripts of the signalling pathway-related genes Keap-1, TOR, and S6K as well as the longevity gene MTH were significantly upregulated to maximum levels. This corrected mortality reached 55.49%, with the lowest performance in terms of mean lifespan, maximal mean lifespan, median lethal time and flyability (15.00%) observed in the offspring at 24 h. In the group receiving a combination of A. virgineoides + P. rubicundus + L. nuda, the adult pupae quantity, adult fly quantity, and adult fly rate were the lowest at 14.33%, 4.00%, and 27.63%, respectively. In addition, the adult fly rate reached only 32.32% of that of the control group, and the highest aberration rate (47.78%) was also observed in the offspring. The mycelia of A. virgineoides, P. rubicundus, and L. nuda all demonstrated toxicity against D. melanogaster, and the combinations of different macrofungal mycelia had a synergistic effect regarding their toxicity. The data reported in the present study—particularly when using A. virgineoides and P. rubicundus with potential insecticidal activity against D. melanogaster—are novel.
Graphical Abstract
2D resistivity model around the rupture area of the 2011 Tohoku-oki earthquake (Mw 9.0)
Authors: Ichihara Hiroshi , Kasaya Takafumi , Baba Kiyoshi , Goto Tada-nori , Yamano Makoto 
Published: May 2023

AbstractThe 2011 Tohoku-oki earthquake (Mw 9.0) was characterized by a huge fault slip on the shallowest part of the plate interface, where fault behavior had been believed to be aseismic. In this study, we modeled the two-dimensional resistivity distribution across the slip area based on ocean-bottom electromagnetic measurements to understand the physical properties around the plate interface controlling fault rupture processes. The optimal 2D resistivity model showed a conductive area around the shallowest plate interface where the huge coseismic slip was observed, whereas the deeper plate interface where the fault rupture was nucleated was relatively more resistive. The shallowest plate interface was interpreted to have a high pore seawater fraction, whereas the deeper interface was interpreted as a dry area. These findings are consistent with the hypothesis that aseismic frictional conditions changed to conditions enhancing fault rupture when the rupture propagated to the wet, clay-rich shallowest plate area. The optimal resistivity model also revealed a conductive area under the outer-rise area of the Pacific Plate. This finding supports the existence of a hydrated oceanic crust that supplied aqueous water to the subduction zone, including to the huge fault slip area.
Graphical Abstract
Negotiating filial care in transitions: an ethnographic study of family involvement in China’s nursing homes
Authors: Wu Xinyue 
Published: May 2023

AbstractBased on ethnographic research conducted in two nursing homes in China, this article examines how institutional eldercare reshapes the expectations and practices of filial piety. It finds that families accept institutional care as a solution to the elderly care deficit. They expect a new division of care between labor and love, assigned to paid care workers and family members, respectively. This ideal of care division is rooted in the “intimate turn” in Chinese family life. Nevertheless, many family members go beyond this care division and remain deeply involved in nursing homes. On the one hand, adult children take on the responsibility to manage surrogate caretakers to improve the quality of care. On the other hand, they continue to provide personal care and companionship. Sharing family time is made the highest priority, especially in the face of impending death. This study goes beyond the binary division between commercial care and family care and sheds light on the transformation of filial piety in the commodification of eldercare in contemporary China.
Induction chemotherapy and hepatic artery embolization followed by extended resection for locally advanced gallbladder cancer: a case report
Authors: Takagi Chisato , Sato Michio , Tomita Masato , Sugita Atsushi , Tokuda Toshiki , Fujiwara Koki , Ando Nobutoshi 
Published: May 2023

AbstractBackgroundSurgical resection plays a critical role in the curative therapy of patients with gallbladder cancer. However, extended resection for locally advanced gallbladder cancer is a controversial procedure because of the high operative morbidity, mortality, and poor prognosis after surgery, without consensus of its suitability. Several reports have described preoperative treatment modalities to reduce the risk of mortality and morbidity and improve the curability of surgery for locally advanced GBCA. However, only a few well-designed studies have verified the benefits of these preoperative strategies.Case presentationA 62-year-old male patient presented to our department with a gallbladder tumor detected on abdominal ultrasound during an annual medical checkup. Multi-phase enhanced CT revealed a gallbladder tumor with a maximum diameter of 34 mm, invading the right hepatic artery, pancreatic head, hepatic flexure of the colon, and first portion of the duodenum. We diagnosed gallbladder carcinoma as cT4 cN0 cM0 cStage IVA in the Union for International Cancer Control (UICC) classification 8th edition. After administration of 12 cycles of gemcitabine and cisplatin plus S-1 regimen, tumor shrinkage was observed on computed tomography, and elevated serum CA19-9 levels were reduced to normal limits. After preoperative hepatic artery embolization, we performed gallbladder bed resection with pancreaticoduodenectomy (minor hepatopancreatoduodenectomy) and combined resection of the right hepatic artery and hepatic flexure of the colon. Histological examination revealed no evidence of lymph node metastasis (ypT4 ypN0 ycM0 yp Stage IVA in the 8th edition of the UICC). The proximal bile duct and dissected margins were negative.ConclusionsThe combination of induction chemotherapy and preoperative hepatic artery embolization, followed by minor hepatopancreatoduodenectomy and combined resection of the involved arteries and partial colon, could be a feasible treatment strategy for patients with locally advanced gallbladder cancer invading neighboring organs.