bluuebunny/crossref_metadata_2025_split huggingface dataset:
Upregulation of complement proteins in lung cancer cells mediates tumor progression
Authors: Kleczko Emily K. , Poczobutt Joanna M. , Navarro Andre C. , Laskowski Jennifer , Johnson Amber M. , Korpela Sean P. , Gurule Natalia J. , Heasley Lynn E. , Hopp Katharina , Weiser-Evans Mary C.M. , Gottlin Elizabeth B. , Bushey Ryan T. , Campa Michael J. , Patz Edward F. , Thurman Joshua M. , Nemenoff Raphael A. 
Published: January 2023

IntroductionIn vivo, cancer cells respond to signals from the tumor microenvironment resulting in changes in expression of proteins that promote tumor progression and suppress anti-tumor immunity. This study employed an orthotopic immunocompetent model of lung cancer to define pathways that are altered in cancer cells recovered from tumors compared to cells grown in culture.MethodsStudies used four murine cell lines implanted into the lungs of syngeneic mice. Cancer cells were recovered using FACS, and transcriptional changes compared to cells grown in culture were determined by RNA-seq.ResultsChanges in interferon response, antigen presentation and cytokine signaling were observed in all tumors. In addition, we observed induction of the complement pathway. We previously demonstrated that activation of complement is critical for tumor progression in this model. Complement can play both a pro-tumorigenic role through production of anaphylatoxins, and an anti-tumorigenic role by promoting complement-mediated cell killing of cancer cells. While complement proteins are produced by the liver, expression of complement proteins by cancer cells has been described. Silencing cancer cell-specific C3 inhibited tumor growth In vivo. We hypothesized that induction of complement regulatory proteins was critical for blocking the anti-tumor effects of complement activation. Silencing complement regulatory proteins also inhibited tumor growth, with different regulatory proteins acting in a cell-specific manner.DiscussionBased on these data we propose that localized induction of complement in cancer cells is a common feature of lung tumors that promotes tumor progression, with induction of complement regulatory proteins protecting cells from complement mediated-cell killing.
Skeletal muscle metastases in neuroblastoma share common progenitors with primary tumor and biologically resemble stage MS disease
Authors: Fong Christina , Kushner Brian H. , Di Giannatale Angela , Gundem Gunes , Li Shanita , Roberts Stephen S. , Basu Ellen M. , Price Anita , Cheung Nai-Kong V. , Modak Shakeel 
Published: January 2023

IntroductionWhile subcutaneous metastases are often observed with stage MS neuroblastoma, an entity that usually resolves spontaneously, skeletal muscle metastases (SMM) have been rarely described. The purpose of this retrospective study was to investigate the significance of SMM in neuroblastoma.Patients and methodsSeventeen patients with neuroblastoma SMM were diagnosed at a median age of 4.3 (0.1-15.6) months. All had SMM at diagnosis and metastases at other sites. Fifteen (88%) had ≥ 2 SMM in disparate muscle groups. One, 14, and 2 patients had low, intermediate, and high-risk disease respectively. Fifteen tumors had favorable histology without MYCN amplification, and 2 were MYCN-amplified. Most SMM (80%; n=12/15 evaluated) were MIBG-avid.ResultsOnly 1 patient (with MYCN-non-amplified neuroblastoma) had disease progression. All survive at median follow-up of 47.9 (16.9-318.9) months post-diagnosis. Biological markers (histology, chromosomal and genetic aberrations) were not prognostic. Whole genome sequencing of 3 matched primary and SMM lesions suggested that both primary and metastatic tumors arose from the same progenitor. SMM completely resolved in 10 patients by 12 months post-diagnosis. Of 4 patients managed with watchful observation alone without any cytotoxic therapy, 3 maintain complete remission with SMM resolving by 5, 13, and 21 months post-diagnosis respectively.ConclusionsChildren with neuroblastoma SMM have an excellent prognosis, with a clinical course suggestive of stage MS disease. Based on these results, the initial management of infants with non-MYCN-amplified NB with SMM could be watchful observation, which could eliminate or reduce exposure to genotoxic therapy.
Construction and validation of a prognostic model for hepatocellular carcinoma: Inflammatory ferroptosis and mitochondrial metabolism indicate a poor prognosis
Authors: Han Fang , Cao Dan , Zhu Xin , Shen Lianqiang , Wu Jia , Chen Yizhen , Xu Youyao , Xu Linwei , Cheng Xiangdong , Zhang Yuhua 
Published: January 2023

BackgroundAn increasing number of innovations have been discovered for treating hepatocellular carcinoma (HCC or commonly called HCC) therapy, Ferroptosis and mitochondrial metabolism are essential mechanisms of cell death. These pathways may act as functional molecular biomarkers that could have important clinical significance for determining individual differences and the prognosis of HCC. The aim of this study was to construct a stable and reliable comprehensive model of genetic features and clinical factors associated with HCC prognosis.MethodsIn this study, we used RNA-sequencing (fragments per kilobase of exon model per million reads mapped value) data from the Cancer Genome Atlas (TCGA) database to establish a prognostic model. We enrolled 104 patients for further validation. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes enrichment analyses (KEGG) analysis were used for the functional study of differentially expressed genes. Pan-cancer analysis was performed to evaluate the function of the Differentially Expressed Genes (DEGs). Thirteen genes were identified by univariate and least absolute contraction and selection operation (LASSO) Cox regression analysis. The prognostic model was visualized using a nomogram.ResultsWe found that eight genes, namely EZH2, GRPEL2, PIGU, PPM1G, SF3B4, TUBG1, TXNRD1 and NDRG1, were hub genes for HCC and differentially expressed in most types of cancer. EZH2, GRPEL2 and NDRG1 may indicate a poor prognosis of HCC as verified by tissue samples. Furthermore, a gene set variation analysis algorithm was created to analyze the relationship between these eight genes and oxidative phosphorylation, mitophagy, and FeS-containing proteins, and it showed that ferroptosis might affect inflammatory-related pathways in HCC.ConclusionEZH2, GRPEL2, NDRG1, and the clinical factor of tumor size, were included in a nomogram for visualizing a prognostic model of HCC. This nomogram based on a functional study and verification by clinical samples, shows a reliable performance of patients with HCC.
A self-reported cross-sectional study on the oral function and the quality of life in children with stunted growth
Authors: Sianturi Eunike , Primarti Risti Saptarini , Setiawan Arlette Suzy 
Published: January 2023

BackgroundFailure to catch up with growth in toddlerhood will stunt elementary school children, which continues to cognitive decline and oral motor coordination. Verbal motor skills play an essential role in the oral function of the stomatognathic system, which includes mastication, swallowing, and speech. Therefore, early attention to oral function disorders of the stomatognathic system can avoid complications in children's nutritional status and quality of life.ObjectiveThe objective of this study was to analyze the correlation of oral function of the stomatognathic system with Oral Health-Related Quality of Life (OHRQoL) in stunting children.MethodsThis cross-sectional study correlates with 58 children aged 7–12 years with a history of stunting in toddlerhood from the Pasir Jambu District, Bandung Regency. The oral function of the stomatognathic system was evaluated by Adapted Orofacial Myofunctional Assessment Protocol and OHRQoL with Child Oral Health Impact Profile Short Form (COHIP-SF 19).ResultsThe results were statistically analyzed using Spearman's rank correlation and Kendall’s coefficient of concordance correlation tests. The results showed that the research subjects had a significant relationship in each variable and the three variables of the oral function of the stomatognathic system (chewing, swallowing, and speech) with OHRQoL with a p-value &lt;0.05. The study concluded that the oral function of the stomatognathic system (chewing, swallowing, and speech) is related to OHRQoL in children with stunted growth.
Multisystem inflammatory syndrome in children (MIS-C) and “Near MIS-C”: A continuum?
Authors: Khafaja Sarah , Youssef Nour , El Zein Zeinab , Boutros Celina F. , Bou Karroum Samer , Abdel-Halim Nour , Salameh Rim , Hodroj Daniella , El Meski Nour , Nasrallah Oussama , Bidikian Aram , Bou Saba Ghassan , Arabi Mariam T. , Hanna-Wakim Rima , Dbaibo Ghassan S. 
Published: January 2023

IntroductionReports of multisystem inflammatory syndrome in children (MIS-C), following severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection, have been increasing worldwide, with an incidence varying significantly across studies based on the definition used for the diagnosis. At our tertiary medical center in Lebanon, we encountered several cases that presented a diagnostic challenge because they mimicked MIS-C but did not meet the US Centers for Disease Control and Prevention (CDC) definition. We decided to review these cases and describe their features in comparison with cases that met the CDC criteria of MIS-C and those that had an alternative diagnosis.MethodsThis is a retrospective chart review of subjects aged &lt;19 years old admitted to the American University of Beirut Medical Center (AUBMC) between March 1, 2020, and May 31, 2021, with suspected or confirmed MIS-C, following documented COVID-19 infection, with sufficient or insufficient criteria for diagnosis. Subjects were classified into 3 groups: “MIS-C”, “Near MIS-C” and “Alternative Diagnosis”.ResultsA total number of 29 subjects were included in our cohort. Fever was present in all subjects. In the MIS-C group, evidence for cardiovascular system involvement was the most common feature followed by the mucocutaneous and gastrointestinal systems. In the “Near MIS-C” and “Alternative Diagnosis” group, gastrointestinal symptoms were the most common with only one patient with cardiac abnormalities and none with coagulopathy. Subjects with typical MIS-C presentation had higher inflammatory markers when compared to subjects in the other groups. Almost all the subjects had positive IgG for SARS-CoV-2. Of the 29 subjects, the Royal College of Paediatrics and Child Health (RCPCH) case definition would have identified all suspected cases without an alternative diagnosis as MIS-C, whereas the World Health Organization (WHO) and the CDC definitions would have excluded 6 and 10 subjects, respectively.ConclusionMIS-C presents a diagnostic challenge due to the nonspecific symptoms, lack of pathognomonic findings, and potentially fatal complications. More research is needed to fully understand its pathogenesis, clinical presentation spectrum, and diagnostic criteria. Based on our experience, we favor the hypothesis that MIS-C has a continuum of severity that necessitates revisiting and unifying the current definitions.
Pharmacokinetics effects of chuanxiong rhizoma on warfarin in pseudo germ-free rats
Authors: Li Haigang , Zhou Yi , Liao Luanfeng , Tan Hongyi , Li Yejun , Li Zibo , Zhou Bilan , Bao Meihua , He Binsheng 
Published: January 2023

Aim: In China, warfarin is usually prescribed with Chuanxiong Rhizoma for treating thromboembolism diseases. However, the reason for their combination is still being determined. The present study explored the pharmacokinetics interactions of warfarin, Chuanxiong Rhizoma, and gut microbiota in the rat model of middle cerebral artery occlusion (MCAO).Methods: A total of 48 rats were randomly divided into six groups: MCAO rats orally administered warfarin (W group), pseudo germ-free MCAO rats orally administered warfarin (W-f group), MCAO rats co-administered Chuanxiong Rhizoma and warfarin (C + W group), pseudo germ-free MCAO rats co-administered Chuanxiong Rhizoma and warfarin (C + W-f group), MCAO rats co-administered warfarin and senkyunolide I (S + W group); pseudo germ-free MCAO rats co-administered warfarin and senkyunolide I (S + W-f group). After treatment, all animals’ blood and stool samples were collected at different time points. The stool samples were used for 16S rRNA sequencing analysis. Ultra-performance liquid chromatography coupled to tandem mass spectrometry (UPLC-MS/MS) method was established to quantify warfarin, internal standards, and the main bioactive components of Chuanxiong in blood samples. The main pharmacokinetics parameters of warfarin were calculated by DAS 2.1.1 software.Results: The relative abundance of Allobaculum and Dubosiella in the pseudo germ-free groups (W-f, C + W-f, S + W-f) was lower than that in the other three groups (W, C + W, S + W). The relative abundance of Lactobacillus in the W-f group was higher than that of the W group, while the relative abundance of Akkermansia decreased. The relative abundance of Ruminococcaceae_UCG-014 and Ruminococcaceae_NK4A214_group in the S + W-f group was lower than in the S + W group. Compared to the W group, the AUC0-t and Cmax of warfarin in the W-f group increased significantly to 51.26% and 34.58%, respectively. The AUC0-t and Cmax in the C + W group promoted 71.20% and 65.75% more than the W group. Compared to the W group, the AUC0-t and Cmax increased to 64.98% and 64.39% in the S + W group.Conclusion: Chuanxiong Rhizoma and senkyunolide I (the most abundant metabolites of Chuanxiong Rhizoma aqueous extract) might affect the pharmacokinetics features of warfarin in MCAO rats through, at least partly, gut microbiota.
Tipping points of ancient Japanese Jomon trade networks from social network analyses of obsidian artifacts
Authors: Sakahira Fumihiro , Tsumura Hiroomi 
Published: January 2023

The Jomon period of ancient Japan, characterized by hunting and gathering, lasted from 16,000 to 2,400 years cal BP. In this ∼13,000-year period, the geographical range of trade is known to have been extensive but may not have always been constant. We conducted obsidian social network analyses on a large dataset to explore the dynamics of trade networks and their tipping points during the Jomon period. This study clustered sites by geographical location and summarized obsidian artifacts in aggregate values by region to increase regional representativeness. This improved the readability and interpretability of the analysis results and decreased the distortion of results owing to a small sample of sites. The results showed that, for sites adjacent to one another, it is reasonable to group the total values by region and assess the regional representativeness of the findings. Framing the provenance and consumption areas as a bipartite graph and using network analyses among consumption areas revealed that the obsidian trade network expanded throughout the Kanto region in the middle Jomon period (5,500–4,500 years cal BP) but regionalized in the late Jomon period (4,500–3,200 years cal BP). These periods were extracted as tipping points in the Jomon trade network. The timing of these tipping points possibly occurred during a period of major climate change. Therefore, these tipping points of obsidian trade networks may have resulted from population decline and migration caused by shifting coastlines and living infrastructure owing to climate change.
An unstable 0D model of ionization oscillations in Hall thruster plasmas
Authors: Leporini L. , Giannetti V. , Camarri S. , Andreussi T. 
Published: January 2023

The breathing mode is an instability typical of Hall thrusters, which is characterized by oscillations of the discharge current with amplitude of the order of its mean value and frequency in the 5–30 kHz range. The strong link between this instability and the ionization processes is generally recognized. If, on one hand, 1D simulations have shown to be able to reproduce the breathing mode, on the other hand 0D models fell short in recovering self sustained oscillations, making it hard to identify the core physical mechanism governing their formation. In this work an original 0D model is presented and characterized by means of linear stability analysis and direct numerical integration. The electric field is allowed to vary in response to variations of the neutral density, acting on the ionization rate via the electron temperature and the ion dynamics. It is shown that the model is able to reproduce self-sustained oscillations with the typical characteristics of the breathing mode, even when fluctuations of the electron temperature are neglected. The stability of the model is strictly determined by the rigidity with which variations of neutral density reflect into variations of electron mobility.
The effect of loss of foot sole sensitivity on H-reflex of triceps surae muscles and functional gait
Authors: Zhang Fangtong , Sun Mengzi , Qu Feng , Lewis Kelsey , Choi Jung Hun , Song Qipeng , Li Li 
Published: January 2023

Objective: To investigate the effects of foot sole insensitivity on the outcomes of the triceps surae muscle H-reflex and functional gait.Material and Methods: People with peripheral neuropathy were recruited and divided into two groups: people with more (n = 13, 73.3 ± 4.3 years old) or less (n = 10, 73.5 ± 5.3) sensitive tactile sensation. Their monofilament testing scores were 9.0 ± 1.5 (range: 7–10) and 2.3 ± 2.4 (range: 0–6) out of 10, respectively. H-reflex of the triceps surae muscles during quiet standing and their relationship with functional gait, 6 min walking distance (6MWD), and timed-up-and-go duration (TUG), were compared between groups.Results: No significant difference was detected for H-reflex parameters between the groups. The less sensitive group showed reduced (p &lt; .05) functional gait capacity compared to the other group, 38.4 ± 52.7 vs. 463.5 ± 47.6 m for 6MWD, and 9.0 ± 1.5 vs. 7.2 ± 1.1s for TUG, respectively. A significant correlation (p &lt; .05), worse functional gait related to greater H/M ratio, was observed in the less sensitive group, not the other group.Conclusion: Although there was no significant H-reflex difference between the groups, more pronounced tactile sensation degeneration affected functional gaits and their relationship with H-reflex.
Integration of organics in nutrient management for rice-wheat system improves nitrogen use efficiency via favorable soil biological and electrochemical responses
Authors: Bhardwaj Ajay Kumar , Malik Kapil , Chejara Sukirtee , Rajwar Deepika , Narjary Bhaskar , Chandra Priyanka 
Published: January 2023

IntroductionThe contrasting soil management in flooded-transplanted rice (Oryza sativa) and dry-tilled wheat (Triticum aestivum) poses a challenge for improving low nitrogen use efficiency (NUE) of the rice-wheat system. Integration of organics in nutrient management can bring in changes favoring efficient N uptake via changes in growing conditions and soil responses.Materials and methodsThis study reported the results of a 15-year-long experiment on integrated nutrient management (INM) systems for rice-wheat cropping. The INM included substituting ~50% of chemical fertilizers via (i) including a legume crop (Vigna radiata) in the sequence and its biomass incorporation (LE), (ii) green manuring with Sesbania aculeata (GM), (iii) farmyard manure application (FYM), (iv) 1/3 wheat stubble in situ retention (WS), and (v) 1/3 rice stubble in situ retention.Results and DiscussionThe INM strategies resulted in improved NUE compared to 100% chemical fertilizers (F). The INM had significantly higher net N mineralization and improved biological activity aligning with the NUE trends. The reductions in redox potential (Eh) and pH during rice season improved NUE under integrated management. Highly reduced conditions favored N mineralization and plant availability in form of NH4+−N resulting in enhanced uptake efficiency, in rice crop. The soil organic carbon (C) significantly increased in INM, and an effect of the active C fractions was evident on the NUE of the wheat crop.ConclusionThe results showed that these INM strategies can immensely benefit the rice-wheat system via improvement in biological health along with electrochemical changes for flooded rice, and labile-C-assisted improvement in soil conditions for wheat.
Effect of monopolar capacitive resistive radiofrequency in treating stress urinary incontinence: A pilot randomized control trial
Authors: Elhosary Eman A. , Ahmed Hamada Hamada , Ali AlMubali Fatimah , López Sánchez Guillermo F. , Ahmed Sara M. 
Published: January 2023

ObjectiveTo assess the effectiveness of 448 kHz monopolar capacitive resistive radiofrequency (MCRR) in the treatment of females with stress urinary incontinence (SUI).Materials and methodsForty females with SUI complaints were separated randomly into two equal groups. Group A with 20 females received the MCRR therapy for 20 min and performed pelvic floor exercises for 20 min. Group B with 20 females received placebo treatment by applying the same application as in Group A without emitting any waves for 20 min, three times a week, for 4 weeks. The patients in both groups were instructed to pause the treatment during their menstruation; the patients were instructed to maintain home pelvic floor exercises. Both groups were assessed by a perineometer that was used to assess the strength of the pelvic floor muscles (PFM), the visual analogue scale (VAS), and the Incontinence Symptom Severity Index to assess the frequency of urinary incontinence symptoms as described by each patient before treatment and after 4 weeks of treatment.ResultsThere was a significant reduction (p &lt; 0.05) in VAS and the Incontinence Symptom Severity Index and a significant increase in the strength of the PFM in both groups post-treatment compared with the pre-treatment. Regarding between-subject effects, there was a significant difference in VAS, the Incontinence Symptom Severity Index, and the perineometer between both groups (p &lt; 0.05), and this significant improvement favored Group A.ConclusionMCRR and pelvic floor exercises are more effective methods for the treatment of SUI than just pelvic floor exercises of females with SUI.Clinical Trial RegistrationClinicalTrials.gov. Identifier: NCT04612205.