bluuebunny/crossref_metadata_2025_split huggingface dataset:
Nitric Oxide‐Releasing Bioinspired Scaffold for Exquisite Regeneration of Osteoporotic Bone via Regulation of Homeostasis
Authors: Lee Jun‐Kyu , Kim Da‐Seul , Park So‐Yeon , Baek Seung‐Woon , Jung Ji‐Won , Kim Tae‐Hyung , Han Dong Keun 
Published: December 2022

AbstractOsteoporotic bone regeneration is a challenging process which involves the occurrence of sophisticated interactions. Although various polymeric scaffolds have been proposed for bone repair, research on osteoporotic bone regeneration remains practically limited. In particular, achieving satisfactory bone regeneration when using osteoporotic drugs is challenging including bisphosphonates. Here, a novel nitric oxide‐releasing bioinspired scaffold with bioactive agents for the exquisite regeneration of osteoporotic bone is proposed. The bone‐like biomimetic poly(lactic‐co‐glycolic acid) scaffold is first prepared in combination with organic/inorganic ECM and magnesium hydroxide as the base implant material. Nanoparticles containing bioactive agents of zinc oxide (ZO), alendronate, and BMP2 are incorporated to the biomimetic scaffold to impart multifunctionality such as anti‐inflammation, angiogenesis, anti‐osteoclastogenesis, and bone regeneration. Especially, nitric oxide (NO) generated from ZO stimulates the activity of cGMP and protein kinase G; in addition, ZO downregulates the RANKL/osteoprotegerin ratio by suppressing the Wnt/β‐catenin signaling pathway. The new bone is formed much better in the osteoporotic rat model than in the normal model through the regulation of bone homeostasis via the scaffold. These synergistic effects suggest that such a bioinspired scaffold could be a comprehensive way to regenerate exceptionally osteoporotic bones.
Resilience and resistance to the accumulation of amyloid plaques and neurofibrillary tangles in centenarians: An age‐continuous perspective
Authors: Zhang Meng , Ganz Andrea B. , Rohde Susan , Rozemuller Annemieke J.M. , Bank Netherlands Brain , Reinders Marcel J.T. , Scheltens Philip , Hulsman Marc , Hoozemans Jeroen J.M. , Holstege Henne 
Published: December 2022

AbstractIntroductionWith increasing age, neuropathological substrates associated with Alzheimer's disease (AD) accumulate in brains of cognitively healthy individuals—are they resilient, or resistant to AD‐associated neuropathologies?MethodsIn 85 centenarian brains, we correlated NIA (amyloid) stages, Braak (neurofibrillary tangle) stages, and CERAD (neuritic plaque) scores with cognitive performance close to death as determined by Mini‐Mental State Examination (MMSE) scores. We assessed centenarian brains against 2131 brains from AD patients, non‐AD demented, and non‐demented individuals in an age continuum ranging from 16 to 100+ years.ResultsWith age, brains from non‐demented individuals reached the NIA and Braak stages observed in AD patients, while CERAD scores remained lower. In centenarians, NIA stages varied (22.4% were the highest stage 3), Braak stages rarely exceeded stage IV (5.9% were V), and CERAD scores rarely exceeded 2 (4.7% were 3); within these distributions, we observed no correlation with the MMSE (NIA: P = 0.60; Braak: P = 0.08; CERAD: P = 0.16).DiscussionCognitive health can be maintained despite the accumulation of high levels of AD‐related neuropathological substrates.Highlights
Cognitively healthy elderly have AD neuropathology levels similar to AD patients.
AD neuropathology loads do not correlate with cognitive performance in centenarians.
Some centenarians are resilient to the highest levels of AD neuropathology.
MGMT, a risk factor for both genetic and environmental forms of dementia
Authors: Kisby Glen E. , Oakes Heaton , Beckett David , Spencer Peter S. 
Published: December 2022

AbstractMGMT, the gene coding for the DNA‐repair protein O6‐methylguanine methyltransferase, which has been recently shown to be a risk factor for inherited forms of Alzheimer's disease (AD), notably among women, might also be linked to Western Pacific amyotrophic lateral sclerosis and Parkinsonism‐dementia complex (ALS/PDC), one phenotype of which is an AD‐like dementia. Guam ALS/PDC is strongly considered to be an environmental disorder caused by oral exposure to natural toxins (i.e., genotoxic/epigenotoxic chemicals), notably methylazoxymethanol (MAM) that alkylates guanine to form O6‐methylguanine, found in the seed of cycad plants traditionally used for food. Thus, the DNA‐repair protein MGMT might participate in both AD and in the AD‐related disorder ALS/PDC.
Fabrication of whey protein isolate/chitosan complexes and its protective effect on allicin
Authors: Ding Shuang , Zhao Jun , Jiang Zhiyuan , Mu Jing , Huang Liurong , Dai Chunhua 
Published: December 2022

AbstractWhey protein isolate (WPI) and chitosan (CS) complexes were prepared by complex coacervation method. The effects of different WPI/CS ratios and pH values on the formation of complexes were investigated. And then the protective effect of the WPI/CS complexes on allicin was further analyzed. The turbidity, particle size and zeta potential data showed that optimum soluble complex coacervation occurred at pH 5.0 when the WPI/CS ratio was 4:1. Electrostatic interaction and hydrogen bonding were the main driving forces involved in the coacervation. Changes in differential scanning calorimetry (DSC) curves indicated that WPI/CS complexes exhibited higher thermal denaturation temperature than WPI. The retention ratio of encapsulated allicin was 71.4% after stored at 4°C for 14 weeks, which was increased by 51.6% as compared with that of free allicin. Due to the denaturation of protein, a high‐temperature (90°C) and long‐time (7 h) treatment would result in a decrease of stability for encapsulated allicin. Results of this study would be helpful for designing food particles, which can be used for encapsulation and protection of bioactive molecules under certain conditions.
Importance of precursor complexation for green solvent‐processed perovskite crystals
Authors: Jo Na‐Yeon , Kim Hui‐Seon 
Published: December 2022

AbstractPerovskite solar cells have received great attention as one of the most promising photovoltaics for commercialization. While tremendous efforts have been made to ensure long‐term stability, green solvent for perovskite solution process have been enormously explored because of an increasing importance of the safety and environmental issues for market entry. Methylammonium propionate (MAP), being an ionic liquid, has been considered as a suitable nonhazardous and green solvent to replace conventional toxic solvents such as dimethylformamide and dimethyl sulfoxide. In this study, the aging effect of perovskite precursor solution based on MAP is investigated, where the formation of dimethylammonium (DMA) during the precursor aging plays a key role in the perovskite growth. A complexation of MAP, PbI6, and DMA is expected to promote the crystal growth toward photo‐active black phase, leading to a superior absorbance of the resultant film compared to that from the as‐prepared precursor solution without aging.
Antigen B modulates anti‐inflammatory cytokines in the EAE model of multiple sclerosis
Authors: Mariki Aliakbar , Barzin Zahra , Fasihi Harandi Majid , Karbasi Ravari Kimia , Davoodi Mahboubeh , Mousavi Seyed Mohammad , Rezakhani Soheila , Nazeri Masoud , Shabani Mohammad 
Published: December 2022

AbstractIntroduction: Multiple sclerosis (MS) is characterized by the destruction of the blood–brain barrier, loss of myelin sheath, and contribution of inflammatory interleukins such as TNF‐alpha, interleukin‐17, and interleukin‐6.Methods: The current study investigated the effect of antigen B of hydatid cyst fluid on the reduction of anti‐inflammatory cytokines and nerve conduction velocity in rats with experimental autoimmune encephalomyelitis (EAE)‐induced MS. After isolation of antigen B from sterile cyst fluid, the rats were randomly divided into four groups: saline, EAE, EAE + teriflunomide (EAE + TF), and EAE + antigen B (EAE + AngB). The EAE model was induced using cow spinal cord homogenization, in combination with Freund's complete adjuvant. The serum concentration of cytokines including IL‐1B and IL‐17, IL‐10, IL‐6, and TNF‐X was measured by the ELISA method, and real‐time PCR was performed to study gene expression. Electrophysiological, behavioral, and neuropathological tests were also conducted.Results: Nerve conduction velocity and IL‐10 concentration were increased in the antigen B group. The results of this study showed that antigen B reduced the inflammatory component of the EAE MS animal model by modulating the immune system compared to teriflunomide, which eventually led to a reduction in symptoms at the behavioral and electrophysiological level.Conclusions: It seems that antigen B plays a critical role in regulating immunity and it can be used as a possible therapeutic agent to modulate the immune system in MS patients. It might be rational to consider hydatid cyst fluid antigen as a modifier in MS.
Antigen rapid diagnostic test monitoring for SARS‐CoV‐2 in asymptomatic and fully vaccinated cancer patients: Is it cost‐effective?
Authors: Caccese Mario , Saieva Anna Maria , Guarneri Valentina , Lonardi Sara , Cacco Massimo , Sileni Vanna Chiarion , Gottardi Michele , Mioranza Eleonora , Bergamo Francesca , Brunello Antonella , Zagonel Vittorina , Benini Patrizia 
Published: December 2022

AbstractBackgroundRoutine testing for cancer patients not presenting COVID‐19‐related symptoms and fully vaccinated for SARS‐CoV‐2 prior to cancer treatment is controversial.MethodsIn this retrospective study we evaluated whether antigen‐rapid‐diagnostic‐test (Ag‐RDT) monitoring for SARS‐CoV‐2 in a large cohort of consecutive asymptomatic (absence of SARS‐CoV‐2‐related symptoms such as fever, cough, sore throat or nasal congestion) and fully vaccinated cancer patients enrolled in a short period during cancer treatment has an impact on the therapeutic path of cancer patients.ResultsFrom December 27, 2021, to February 11, 2022, 2439 cancer patients were screened through Ag‐RDT for SARS‐CoV‐2 before entering the hospital for systemic treatment. Fifty‐three patients (2.17%) tested positive, of whom 7 (13.2%) subsequently developed COVID‐related symptoms, generally mild. Cancer treatment was discontinued, as a precaution, in 49 patients (92.5%) due to the test positivity.ConclusionSARS‐CoV‐2 screening in asymptomatic and fully vaccinated cancer patients during systemic treatment appeared to be not cost‐effective: the low rate of SARS‐CoV‐2 positive patients and the low percentage of overt associated infection do not seem proportional to the direct costs (nursing work for swabs, costs of materials and patient monitoring) and indirect costs (dedicated rooms, extension of waiting times for patients and oncologists in delivering therapy as well as its discontinuation in the positive ones). It can, on the other hand, be detrimental when systemic cancer treatment is suspended as a precaution. Given the small number of patients testing positive and the rapid and favorable trend of the infection, it is recommended to always consider continuing systemic oncological treatment, especially when this impacts patient survival as in the adjuvant or neoadjuvant setting.
Circulating gamma‐glutamyl transpeptidase and risk of pancreatic cancer: A prospective cohort study in the UK Biobank
Authors: Liao Weiting , Yang Yu , Yang Huazhen , Qu Yuanyuan , Song Huan , Li Qiu 
Published: December 2022

AbstractBackgroundTo determine whether serum gamma‐glutamyl transpeptidase (GGT) level is associated with pancreatic cancer risk in a large prospective cohort.MethodsThe study analyzed serum GGT concentration at baseline of 421,032 participants recruited in the UK Biobank since 2006 through 2010. Information on incidence of pancreatic cancer was obtained from cancer and death registers, updated until 2015 in Scotland or 2016 in England and Wales. Adjusted Cox proportional hazards models were used to measure the association between serum GGT and pancreatic cancer risk.ResultsThe study identified 586 cases of pancreatic cancer over a median follow‐up period of 7.16 years. In the multivariable‐adjusted Cox model, serum GGT level was associated with 14% higher pancreatic cancer risk (hazard ratio (HR) per one standard deviation increment of log2 GGT level = 1.14, 95% confidence interval (CI) 1.02–1.28, p = 0.025). In the total population, the HR for the highest GGT group was 1.68 (95%CI: 1.22–2.30) versus the lowest GGT group. The HR for the highest GGT group in men (≥50.2 U/L) was 1.72 (95%CI: 1.14–2.61) and that in women (≥31.6 U/L) was 1.75 (95%CI: 1.06–2.88) versus the lowest GGT group.ConclusionOur findings suggested a positive association of serum GGT in pancreatic cancer etiology, implying the potential of monitoring GGT level for identifying at‐risk individuals for pancreatic cancer.
MFAP2, upregulated by m1A methylation, promotes colorectal cancer invasiveness via CLK3
Authors: Xue Meng , Mi Shuyi , Zhang Zizhen , Wang Hao , Chen Wenwen , Wei Wei , Lou Guochun 
Published: December 2022

AbstractBackgroundDistant metastasis is the main cause of mortality in colorectal cancer (CRC) patients. N1‐methyladenosine (m1A) is a type of epitranscriptome modification. While its regulatory effect on mRNA and its role in CRC metastasis remain unclear.MethodsThe m1A methylation profile of mRNAs in CRC was revealed by m1A methylated RNA immunoprecipitation sequencing. The expression of MFAP2 in tumor tissues was measured by immunohistochemistry and then correlated with the clinical characteristics and prognosis of CRC patients. The role of MFAP2 in the invasiveness of CRC cells was evaluated by transwell assays and peritoneal metastatic model in nude mice. The downstream targets of MFAP2 was screened by mass spectrometry analysis. Then the role of MFAP2‐CLK3 signaling axis was verified by cotransfecting MFAP2 siRNA and CLK3 plasmid in CRC cells.ResultsMicrofibril associated protein 2 (MFAP2) mRNA was overexpressed and m1A‐hypermethylated in CRC. High expression of MFAP2 was closely related to lymph node metastasis and distant metastasis, leading to poor prognosis in patients with CRC. In vivo and in vitro studies showed that silencing of MFAP2 inhibited the migration, invasion and metastasis of CRC cells. CDC Like Kinase 3 (CLK3) was a potential downstream target of MFAP2. Further studies showed that MFAP2 depletion might induce autophagic degradation of CLK3, and the role of MFAP2 in the invasiveness of CRC cells was dependent on CLK3.ConclusionsOur results uncover a newly identified MFAP2‐CLK3 signaling axis, which is a potential therapeutic target for CRC metastasis.
Xanthones and Phenylpropanoids from the Whole Herb of Swertia pseudochinensis and Their Anti‐Inflammatory Activity
Authors: Yan Yushu , Li Yuxia , Sa Kuiru , Sun Dejuan , Li Hua , Chen Lixia 
Published: December 2022

AbstractAn undescribed xanthone dimer, 1,3,5,8‐tetrahydroxy‐7‐(1′,5′,8′‐trihydroxy‐3′‐methoxy‐2′‐xanthonyl)xanthone (1) was separated together with eleven known compounds (2–12) from the dried whole herb of Swertia pseudochinensis. It was the first time that the compounds 8–12 were isolated from the Swertia genus. The structure of compound 1 was illuminated based on chemical evidence and spectral data analysis (UV, 1D and 2D‐NMR, HR‐ESI‐MS). Moreover, the inhibitory effects of all compounds on NO production in LPS‐induced RAW 264.7 cells were tested, compounds 8, 9, 10, 11 and 12 showing significant inhibition. The IC50 value of compound 12 was 3.05±1.10 μM. Using target screening and molecular docking, we hypothesized that compound 12 may bind neutrophil elastase to exert its anti‐inflammatory effects.
IrGa Bimetallic Catalyst with Atomical Metal Dispersion for Propane Dehydrogenation with High Stability
Authors: Zeng Lei , Li Wei , Wei Xueer , Cheng Jiawei , Zhou Wei , Zheng Yanping , Cai Fan , Liu Yuefeng , Cheng Kang , Zhang Qinghong , Wang Ye 
Published: December 2022

AbstractIndustrial catalysts for alkane dehydrogenation to olefins suffer from fast deactivation due to the coking and sintering of active metal under harsh reaction conditions. Herein, iridium, a noble metal rarely applied in C−H activation, has been employed to construct an ultra‐stable IrGa/Al2O3 catalyst with atomical metal dispersion for non‐oxidative propane dehydrogenation (PDH). A synergetic effect between metallic Ir and GaOx is discovered which contributes to both high PDH activity and propylene selectivity. Notably, the Ir0.5Ga2/Al2O3 with 0.5 wt % of Ir and 2.0 wt % of Ga exhibited a propylene selectivity of 98 % at a 19 % propane conversion at 550 °C and 86 % at a 35 % propane conversion at 600 °C. A stable PDH performance of 100 h was observed after a deactivation process at the initial reaction stage. The catalytic performance of the IrGa/Al2O3 is comparable to that of Pt‐based catalysts, endowing the Ir‐based catalyst with great potential in dehydrogenation reactions.
Optimized Reduction of a Graphene Oxide‐MWCNT Composite with Electrochemically Deposited Copper Nanoparticles on Screen Printed Electrodes for a Wide Range of Detection of Nitrate
Authors: Anurag Adiraju , Al‐Hamry Ammar , Attuluri Yashwanth , Palaniyappan Saravanan , Wagner Guntram , Dentel Doreen , Tegenkamp Christoph , Kanoun Olfa 
Published: December 2022

AbstractIn this report, we demonstrate the capability of electrochemically deposited copper (Cu) nanoparticles on electrochemically reduced graphene oxide (ERGO)‐multiwalled carbon nanotubes (MWCNT) composite on screen printed carbon electrodes (SPCE) for electrochemical detection of nitrate. Prior to the detection, extensive fundamental investigations on the electrochemical reduction of GO on SPCE and role of MWCNT in the reduction process and the degree of reduction have been carried out which has not been previously reported. Profiting from the complementary information obtained from electrochemical impedance spectroscopy, Raman spectroscopy, and cyclic voltammetry (CV), the role of MWCNT and optimal number of scans in CV (15 scans) for the reduction was obtained. The determination of nitrate on Cu/ERGO‐MWCNT/SPCE was performed by square wave voltammetry and shows a wide linear range from 10 to 750 μM and low limit of detection of 3.3 μM, thereby enhancing the applicability of the developed electrode in the regions of low and high concentrations as well. The electrode was tested in tap water and the analytical capability was compared using F‐test and matrix effect (4.8 %), which highlights excellent analytical ability of the Cu/ERGO‐MWCNT modified electrodes to detect nitrate.
PIDA‐mediated Oxidative Decarboxylation of Oxamic Acids. The Role of Radical Acidity Enhancement.
Authors: Ogbu Ikechukwu Martin , Kurtay Gülbin , Badufle Margaux , Robert Frédéric , Lopez Carlos Silva , Landais Yannick 
Published: December 2022

AbstractThe PIDA‐mediated oxidative decarboxylation of oxamic acids in the presence of alcohols is shown to afford the corresponding urethanes under thermal conditions. Computational and experimental mechanistic exploration allows to rationalize the different reactivity of PIDA as compared to related cyclic BI‐OAc and highlights the importance of the enhanced acidity of the proton in the carbamoyl radical intermediate.
Stabilization of 2D Imine‐Linked Covalent Organic Frameworks: From Linkage Chemistry to Interlayer Interaction
Authors: Jiang Guoxing , Zou Wenwu , Ou Zhaoyuan , Zhang Weifeng , Liang Zhenxing , Du Li 
Published: December 2022

AbstractImine‐linked covalent organic frameworks (imine‐COFs) represent the most sought‐after class of COFs due to their broad monomer scope and ease of synthesis. Owing to the reversible nature of imine linkages, however, the chemical stability of most imine‐COFs is still far from adequate. In this context, emerging strategies, ranging from linkage chemistry to interlayer interaction, have been employed to construct stable imine‐COFs for their applications in electronics, sensing, and energy storage devices. This Concept article summarizes the latest advances aimed at tuning the structural stability of imine‐COFs. Furthermore, this Concept provides a prospective for the precise design of stable imine‐COFs based on the characteristics of structure, physical properties, and chemical functions, as well as the mechanism of structure locking and stabilization during crystal growth.
Incidence of abnormal anal cytology in HIV‐infected and HIV‐uninfected men who have sex with men
Authors: Benevolo Maria , Latini Alessandra , Rollo Francesca , Giuliani Massimo , Giglio Amalia , Giuliani Eugenia , Cristaudo Antonio , Morrone Aldo , Donà Maria Gabriella 
Published: December 2022

AbstractIntroductionAnal cytology is used in the prevention of anal cancer, which disproportionally affects men who have sex with men (MSM). Data on the incidence of cytologic abnormalities in these individuals are scant.MethodsMSM with baseline negative anal cytology and at least one further adequate cytology were included. Incidence rate for positive atypical squamous cells of undetermined significance (ASC‐US+) was calculated. Kaplan–Meier curves were compared by log‐rank test according to HIV status, baseline high‐risk human papillomavirus (HPV) (high‐risk HPV‐negative, HPV16‐positive, other high‐risk HPV‐positive [non‐HPV16]) and high‐risk HPV persistence (positive from baseline to the first ASC‐US+ or last visit for those who remained cytologically negative). Cox univariate and multivariate analyses were performed.ResultsA total of 250 MSM were included: 52/153 (34.0%) HIV‐uninfected MSM had an ASC‐US+ report at follow‐up (incidence: 13.1 × 100 person‐years; 95% CI, 9.8–17.2); 48/97 (49.5%) HIV‐infected MSM developed cytologic abnormalities (incidence: 16.0 × 100 person‐years; 95% CI, 11.8–21.2). ASC‐US+ incidence in HIV‐uninfected and HIV‐infected MSM did not differ significantly (p = .32). Kaplan–Meier curves did not differ significantly according to baseline high‐risk HPV. Differences were significant between those with and without persistent high‐risk HPVs, both among HIV‐uninfected (p = .03) and HIV‐infected MSM (p = .008). Age (adjusted hazard ratio [aHR], 0.98; 95% CI, 0.96–0.99), high‐risk HPV persistence (aHR, 1.57; 95% CI, 1.02–2.39), and condomless receptive anal sex (aHR, 1.99; 95% CI, 1.31–3.03) were predictors for incident ASC‐US+.ConclusionsDespite the limited number of subjects, in our study HIV‐uninfected and HIV‐infected MSM have a similar ASC‐US+ incidence. Occurrence of ASC‐US+ was significantly affected by age, high‐risk HPV persistence, and condomless receptive anal sex. The assessment of HPV persistence might identify those MSM at higher risk for anal lesions.