bluuebunny/crossref_metadata_2025_split huggingface dataset:
Effect of xenon on brain injury, neurological outcome, and survival in patients after aneurysmal subarachnoid hemorrhage—study protocol for a randomized clinical trial
Authors: Laaksonen Mikael , Rinne Jaakko , Rahi Melissa , Posti Jussi P. , Laitio Ruut , Kivelev Juri , Saarenpää Ilkka , Laukka Dan , Frösen Juhana , Ronkainen Antti , Bendel Stepani , Långsjö Jaakko , Ala-Peijari Marika , Saunavaara Jani , Parkkola Riitta , Nyman Mikko , Martikainen Ilkka K. , Dickens Alex M. , Rinne Juha , Valtonen Mika , Saari Teijo I. , Koivisto Timo , Bendel Paula , Roine Timo , Saraste Antti , Vahlberg Tero , Tanttari Juha , Laitio Timo 
Published: June 2023

Abstract
Background
Aneurysmal subarachnoid hemorrhage (aSAH) is a neurological emergency, affecting a younger population than individuals experiencing an ischemic stroke; aSAH is associated with a high risk of mortality and permanent disability. The noble gas xenon has been shown to possess neuroprotective properties as demonstrated in numerous preclinical animal studies. In addition, a recent study demonstrated that xenon could attenuate a white matter injury after out-of-hospital cardiac arrest.

Methods
The study is a prospective, multicenter phase II clinical drug trial. The study design is a single-blind, prospective superiority randomized two-armed parallel follow-up study. The primary objective of the study is to explore the potential neuroprotective effects of inhaled xenon, when administered within 6 h after the onset of symptoms of aSAH. The primary endpoint is the extent of the global white matter injury assessed with magnetic resonance diffusion tensor imaging of the brain.

Discussion
Despite improvements in medical technology and advancements in medical science, aSAH mortality and disability rates have remained nearly unchanged for the past 10 years. Therefore, new neuroprotective strategies to attenuate the early and delayed brain injuries after aSAH are needed to reduce morbidity and mortality.

Trial registration
ClinicalTrials.gov NCT04696523. Registered on 6 January 2021.
EudraCT, EudraCT Number: 2019-001542-17. Registered on 8 July 2020.
Estimating malaria transmission risk through surveillance of human–vector interactions in northern Ghana
Authors: Coleman Sylvester , Yihdego Yemane , Gyamfi Frank , Kolyada Lena , Tongren Jon Eric , Zigirumugabe Sixte , Dery Dominic B. , Badu Kingsley , Obiri-Danso Kwasi , Boakye Daniel , Szumlas Daniel , Armistead Jennifer S. , Dadzie Samuel K. 
Published: June 2023

Abstract
Background
Vector bionomics are important aspects of vector-borne disease control programs. Mosquito-biting risks are affected by environmental, mosquito behavior and human factors, which are important for assessing exposure risk and intervention impacts. This study estimated malaria transmission risk based on vector–human interactions in northern Ghana, where indoor residual spraying (IRS) and insecticide-treated nets (ITNs) have been deployed.

Methods
Indoor and outdoor human biting rates (HBRs) were measured using monthly human landing catches (HLCs) from June 2017 to April 2019. Mosquitoes collected were identified to species level, and Anopheles gambiae sensu lato (An. gambiae s.l.) samples were examined for parity and infectivity. The HBRs were adjusted using mosquito parity and human behavioral observations.

Results
Anopheles gambiae was the main vector species in the IRS (81%) and control (83%) communities. Indoor and outdoor HBRs were similar in both the IRS intervention (10.6 vs. 11.3 bites per person per night [b/p/n]; z = −0.33, P = 0.745) and control communities (18.8 vs. 16.4 b/p/n; z = 1.57, P = 0.115). The mean proportion of parous An. gambiae s.l. was lower in IRS communities (44.6%) than in control communities (71.7%). After adjusting for human behavior observations and parity, the combined effect of IRS and ITN utilization (IRS: 37.8%; control: 57.3%) on reducing malaria transmission risk was 58% in IRS + ITN communities and 27% in control communities with ITNs alone (z = −4.07, P < 0.001). However, this also revealed that about 41% and 31% of outdoor adjusted bites in IRS and control communities respectively, occurred before bed time (10:00 pm). The mean directly measured annual entomologic inoculation rates (EIRs) during the study were 6.1 infective bites per person per year (ib/p/yr) for IRS communities and 16.3 ib/p/yr for control communities. After considering vector survival and observed human behavior, the estimated EIR for IRS communities was 1.8 ib/p/yr, which represents about a 70% overestimation of risk compared to the directly measured EIR; for control communities, it was 13.6 ib/p/yr (16% overestimation).

Conclusion
Indoor residual spraying significantly impacted entomological indicators of malaria transmission. The results of this study indicate that vector bionomics alone do not provide an accurate assessment of malaria transmission exposure risk. By accounting for human behavior parameters, we found that high coverage of ITNs alone had less impact on malaria transmission indices than combining ITNs with IRS, likely due to observed low net use. Reinforcing effective communication for behavioral change in net use and IRS could further reduce malaria transmission.

Graphical Abstract
Prevalence and resistance profile of bacteria isolated from wound infections among a group of patients in upper Egypt: a descriptive cross-sectional study
Authors: Ahmed Eman Farouk , Rasmi Asia Helmi , Darwish Abdou M. A. , Gad Gamal Fadl Mahmoud 
Published: June 2023

Abstract
Aim
This cross-sectional survey aimed to identify aerobic bacteria, antimicrobial resistance, and multi-drug resistance profiles of bacteria isolated from different wound infections among a group of Egyptian patients.

Results
Of 120 positive samples, 170 isolates were identified. Polymicrobial infections were determined in 55% of samples. The dominant Gram-positive isolated strains were Staphylococcus aureus, especially from wound infections because of accidents (71.8%). Piperacillin, methicillin, ampicillin/sulbactam, and amoxicillin/clavulanic acid were all highly resistant to S. aureus and Coagulase-negative Staphylococci. The prevalence of methicillin-resistant S. aureus in wound infections was 89.9%. S. aureus showed superior sensitivity to vancomycin (85.3%) and linezolid (81.3%). The highest prevalence of Gram-negative isolates was for Pseudomonas aeruginosa (40%), which was highly sensitive to ciprofloxacin (79.2%) and highly resistant to levofloxacin (83.3%). Several isolates revealed a multi-drug resistance profile (52.4%). The overall MDR rate of Gram-positive and Gram-negative isolates were 50% and 54.9%, respectively.

Conclusion
The prevalence of MRSA isolated from various wound infections and MDR is a warning issue in Upper Egypt. It should implement a health education strategy and hygiene measures to prevent the spread of wound infection-causing organisms in the community.
Circulating plasma NT-proBNP predicts subclinical coronary atherosclerosis on CT angiography among older adults in Uganda
Authors: Shakil Saate S , Temu Tecla M , Kityo Cissy , MMed Geoffrey Erem MBChB , Bittencourt Marcio S , Longenecker Chris T 
Published: June 2023

Abstract
Objective
Phenotypes and mechanisms of cardiovascular disease (CVD) may differ across global populations. In sub-Saharan Africa (SSA), distinct environmental determinants may influence development and progression of atherosclerotic coronary artery disease (CAD).

Methods
We investigated associations between 6 established markers of myocardial stress and subsequent subclinical CAD (sCAD), defined as presence of any atherosclerosis on coronary CT angiography (CCTA) in a 2-year prospective cohort of Ugandan adults enriched for cardiometabolic risk factors (RFs) and HIV. Six plasma biomarkers were measured baseline among 200 participants (50% with HIV) aged ≥ 45 years with ≥ 1 cardiovascular RF. At 2-year follow-up, 132 participants (52% with HIV) who returned underwent coronary CCTA.

Results
In logistic regression models adjusted for cardiovascular RFs (age, diabetes, hypertension, hyperlipidemia, smoking, obesity) and non-traditional RFs (HIV, chronic kidney disease), only NT-proBNP predicted subsequent subclinical CAD (p < 0.008, Bonferroni correction for multiple testing). In sensitivity analyses adjusted for ASCVD risk category (instead of individual RFs) in the baseline cohort with multiple imputation applied to missing year 2 CCTA data (n = 200), NT-proBNP remained significantly associated with subsequent CAD (p < 0.008).

Conclusions
NT-proBNP consistently predicted subclinical CAD in Uganda in the absence of such an association among other markers of myocardial stress, suggesting a role for NT-proBNP in atherosclerosis independently of coronary microvascular dysfunction.
An overview of meta-analyses on radiomics: more evidence is needed to support clinical translation
Authors: Zhong Jingyu , Lu Junjie , Zhang Guangcheng , Mao Shiqi , Chen Haoda , Yin Qian , Hu Yangfan , Xing Yue , Ding Defang , Ge Xiang , Zhang Huan , Yao Weiwu 
Published: June 2023

Abstract
Objective
To conduct an overview of meta-analyses of radiomics studies assessing their study quality and evidence level.

Methods
A systematical search was updated via peer-reviewed electronic databases, preprint servers, and systematic review protocol registers until 15 November 2022. Systematic reviews with meta-analysis of primary radiomics studies were included. Their reporting transparency, methodological quality, and risk of bias were assessed by PRISMA (Preferred Reporting Items for Systematic reviews and Meta-Analyses) 2020 checklist, AMSTAR-2 (A MeaSurement Tool to Assess systematic Reviews, version 2) tool, and ROBIS (Risk Of Bias In Systematic reviews) tool, respectively. The evidence level supporting the radiomics for clinical use was rated.

Results
We identified 44 systematic reviews with meta-analyses on radiomics research. The mean ± standard deviation of PRISMA adherence rate was 65 ± 9%. The AMSTAR-2 tool rated 5 and 39 systematic reviews as low and critically low confidence, respectively. The ROBIS assessment resulted low, unclear and high risk in 5, 11, and 28 systematic reviews, respectively. We reperformed 53 meta-analyses in 38 included systematic reviews. There were 3, 7, and 43 meta-analyses rated as convincing, highly suggestive, and weak levels of evidence, respectively. The convincing level of evidence was rated in (1) T2-FLAIR radiomics for IDH-mutant vs IDH-wide type differentiation in low-grade glioma, (2) CT radiomics for COVID-19 vs other viral pneumonia differentiation, and (3) MRI radiomics for high-grade glioma vs brain metastasis differentiation.

Conclusions
The systematic reviews on radiomics were with suboptimal quality. A limited number of radiomics approaches were supported by convincing level of evidence.

Clinical relevance statement
The evidence supporting the clinical application of radiomics are insufficient, calling for researches translating radiomics from an academic tool to a practicable adjunct towards clinical deployment.

Graphical Abstract
Integrating host transcriptomic signatures for distinguishing autoimmune encephalitis in cerebrospinal fluid by metagenomic sequencing
Authors: Fan Siyuan , He Xiangyan , Zhu Zhongyi , Chen Lu , Zou Yijun , Chen Zhonglin , Yu Jialin , Chen Weijun , Guan Hongzhi , Ma Jinmin 
Published: June 2023

AbstractBackgroundThe early accurate diagnoses for autoimmune encephalitis (AE) and infectious encephalitis (IE) are essential since the treatments for them are different. This study aims to discover some specific and sensitive biomarkers to distinguish AE from IE at early stage to give specific treatments for good outcomes.ResultsWe compared the host gene expression profiles and microbial diversities of cerebrospinal fluid (CSF) from 41 patients with IE and 18 patients with AE through meta-transcriptomic sequencing. Significant differences were found in host gene expression profiles and microbial diversities in CSF between patients with AE and patients with IE. The most significantly upregulated genes in patients with IE were enriched in pathways related with immune response such as neutrophil degranulation, antigen processing and presentation and adaptive immune system. In contrast, those upregulated genes in patients with AE were mainly involved in sensory organ development such as olfactory transduction, as well as synaptic transmission and signaling. Based on the differentially expressed genes, a classifier consisting of 5 host genes showed outstanding performance with an area under the receiver operating characteristic (ROC) curve (AUC) of 0.95.ConclusionsThis study provides a promising classifier and is the first to investigate transcriptomic signatures for differentiating AE from IE by using meta-transcriptomic next-generation sequencing technology.
How can ecosystem engineer plants boost productivity in east Mediterranean drylands
Authors: Constantinou Elena , Sarris Dimitrios , Psichoudaki Magda , Cabello Javier , Vogiatzakis Ioannis N. 
Published: June 2023

Abstract
Background
Water availability is the key limiting factor for plant productivity in drylands covering ca. 40% of Earth’s land surface. For such ecosystems to retain productivity and biodiversity under climatic change, it would be valuable to identify/promote keystone plant species that (i) have developed strategies to more efficiently utilize moisture resources not easily accessible and (ii) improve moisture conditions for neighboring plants. The very deep-rooted Ziziphus lotus, considered an ecosystem engineer, is one such example. However, it is not known which biotic traits: (a) canopy interception of moisture/rainfall, (b) hydraulic redistribution of deep ground moisture by roots, or non-biotic factors: (c) soil’s volume, and (d) organic matter content, Z. lotus activates/modulates to play such a role. We, thus, selected dryland ecosystems where the plant dominates and measured for potential effects on the less deep-rooted Thymbra capitata. For assessing impacts on ecosystem productivity, we measured the spatial aggregation of ca. 3600 T. capitata plants. As a proxy for soil moisture availability and its spatial variability, we conducted a 7-year-long study using thymes’ nighttime rehydration. Sampling extended up to 15 m away from Z. lotus.

Results
The density of T. capitata plants growing up to 5 m around Z. lotus vs. thymes growing 10–15 m away was found significantly increased (2.5–4.5 times), while their stem/leaf moisture was ca. 10% higher at predawn compared to nightfall during the dry season. This suggests that ecosystem productivity is driven by a greater soil moisture availability around Z. lotus permitting more thyme daytime transpiration, in contrast to thymes growing further away. The phenomenon appeared only under dry topsoil (during the dry season; becoming stronger during dry years). Morning dew/rainfall interception from the canopy or soil depth/organic matter did not show significant effects, leaving only the hydraulic lift properties of Z. lotus as the most likely driver for soil moisture availability.

Conclusions
The deep-rooting properties and hydraulic lift potential of Z. lotus may be the key in permitting it to boost ecosystem productivity. Such hydraulic plant traits require more attention as they may prove valuable in combating desertification and restoring ecosystems in arid/semiarid regions threatened by climate change.
Sanitary installations and wastewater plumbing as reservoir for the long-term circulation and transmission of carbapenemase producing Citrobacter freundii clones in a hospital setting
Authors: Hamerlinck Hannelore , Aerssens Annelies , Boelens Jerina , Dehaene Andrea , McMahon Michael , Messiaen Anne-Sophie , Vandendriessche Stien , Velghe Anja , Leroux-Roels Isabel , Verhasselt Bruno 
Published: June 2023

Abstract
Background
Accumulating evidence shows a role of the hospital wastewater system in the spread of multidrug-resistant organisms, such as carbapenemase producing Enterobacterales (CPE). Several sequential outbreaks of CPE on the geriatric ward of the Ghent University hospital have led to an outbreak investigation. Focusing on OXA-48 producing Citrobacter freundii, the most prevalent species, we aimed to track clonal relatedness using whole genome sequencing (WGS). By exploring transmission routes we wanted to improve understanding and (re)introduce targeted preventive measures.

Methods
Environmental screening (toilet water, sink and shower drains) was performed between 2017 and 2021. A retrospective selection was made of 53 Citrobacter freundii screening isolates (30 patients and 23 environmental samples). DNA from frozen bacterial isolates was extracted and prepped for shotgun WGS. Core genome multilocus sequence typing was performed with an in-house developed scheme using 3,004 loci.

Results
The CPE positivity rate of environmental screening samples was 19.0% (73/385). Highest percentages were found in the shower drain samples (38.2%) and the toilet water samples (25.0%). Sink drain samples showed least CPE positivity (3.3%). The WGS data revealed long-term co-existence of three patient sample derived C. freundii clusters. The biggest cluster (ST22) connects 12 patients and 8 environmental isolates taken between 2018 and 2021 spread across the ward. In an overlapping period, another cluster (ST170) links eight patients and four toilet water isolates connected to the same room. The third C. freundii cluster (ST421) connects two patients hospitalised in the same room but over a period of one and a half year. Additional sampling in 2022 revealed clonal isolates linked to the two largest clusters (ST22, ST170) in the wastewater collection pipes connecting the rooms.

Conclusions
Our findings suggest long-term circulation and transmission of carbapenemase producing C. freundii clones in hospital sanitary installations despite surveillance, daily cleaning and intermittent disinfection protocols. We propose a role for the wastewater drainage system in the spread within and between rooms and for the sanitary installations in the indirect transmission via bioaerosol plumes. To tackle this problem, a multidisciplinary approach is necessary including careful design and maintenance of the plumbing system.
Update on the pathological roles of prostaglandin E2 in neurodegeneration in amyotrophic lateral sclerosis
Authors: Nango Hiroshi , Tsuruta Komugi , Miyagishi Hiroko , Aono Yuri , Saigusa Tadashi , Kosuge Yasuhiro 
Published: June 2023

AbstractAmyotrophic lateral sclerosis (ALS) is a progressive neurodegenerative disease characterized by selective degeneration of upper and lower motor neurons. The pathogenesis of ALS remains largely unknown; however, inflammation of the spinal cord is a focus of ALS research and an important pathogenic process in ALS. Prostaglandin E2 (PGE2) is a major lipid mediator generated by the arachidonic-acid cascade and is abundant at inflammatory sites. PGE2 levels are increased in the postmortem spinal cords of ALS patients and in ALS model mice. Beneficial therapeutic effects have been obtained in ALS model mice using cyclooxygenase-2 inhibitors to inhibit the biosynthesis of PGE2, but the usefulness of this inhibitor has not yet been proven in clinical trials. In this review, we present current evidence on the involvement of PGE2 in the progression of ALS and discuss the potential of microsomal prostaglandin E synthase (mPGES) and the prostaglandin receptor E-prostanoid (EP) 2 as therapeutic targets for ALS. Signaling pathways involving prostaglandin receptors mediate toxic effects in the central nervous system. In some situations, however, the receptors mediate neuroprotective effects. Our recent studies demonstrated that levels of mPGES-1, which catalyzes the final step of PGE2 biosynthesis, are increased at the early-symptomatic stage in the spinal cords of transgenic ALS model mice carrying the G93A variant of superoxide dismutase-1. In addition, in an experimental motor-neuron model used in studies of ALS, PGE2 induces the production of reactive oxygen species and subsequent caspase-3-dependent cytotoxicity through activation of the EP2 receptor. Moreover, this PGE2-induced EP2 up-regulation in motor neurons plays a role in the death of motor neurons in ALS model mice. Further understanding of the pathophysiological role of PGE2 in neurodegeneration may provide new insights to guide the development of novel therapies for ALS.
Facing ostracism: micro-coding facial expressions in the Cyberball social exclusion paradigm
Authors: Mulder Rosa H. , Bakermans-Kranenburg Marian J. , Veenstra Johan , Tiemeier Henning , van IJzendoorn Marinus H. 
Published: June 2023

AbstractBackgroundSocial exclusion is often measured with the Cyberball paradigm, a computerized ball-tossing game. Most Cyberball studies, however, used self-report questionnaires, leaving the data vulnerable to reporter bias, and associations with individual characteristics have been inconsistent.MethodsIn this large-scale observational study, we video-recorded 4,813 10-year-old children during Cyberball and developed a real-time micro-coding method measuring facial expressions of anger, sadness and contempt, in a multi-ethnic population-based sample. We estimated associations between facial expressions and self-reported negative feelings, explored associations of child characteristics such as sex and parental national origin with observed and self-reported feelings during social exclusion, and tested associations of observed and self-reported feelings during social exclusion with behavior problems at age 14.ResultsFacial expressions of sadness and anger were associated with self-reported negative feelingsduringthe game, but not with such feelingsafterthe game. Further, girls reported to have had less negative feelings during the game than boys, but no such sex-differences were found in total observed emotions. Likewise, children with parents of Moroccan origin reported less negative feelings during the game than Dutch children, but their facial expressions did not indicate that they were differently affected. Last, observed emotions related negatively to later internalizing problems, whereas self-report on negative feelings during the game related positively to later internalizing and externalizing problems.ConclusionsWe show that facial expressions are associated with self-reported negative feelings during social exclusion, discuss that reporter-bias might be minimized using facial expressions, and find divergent associations of observed facial expressions and self-reported negative feelings with later internalizing problems.
Metal Trade and National Integration: bronze technology and metal resources of Yue Style Bronzes from Hunan (8 ~ 5 C. BCE)
Authors: Ma Jiangbo , Wu Xiaotong , Yan Xiansheng 
Published: June 2023

Abstract
A large number of Yue style bronzes with regional cultural characteristics were unearthed in Hunan, which is of great significance for studying the cross-regional circulation of bronze technology and metal resources in the south of the Yangtze River during the Late Bronze Age (8 ~ 5 C. BCE) in China. In this study, 30 Yue style bronzes and 3 Chu style bronzes unearthed from five regions in Hunan Province were analyzed for chemical composition, metallography and lead isotopes. The results show that the alloy materials of Hunan Yue style bronze ware are diverse. The containers are mainly leaded tin bronze, with both tin bronze and copper. The weapons or tools are mostly tin bronze, and the alloy composition is primarily tin. The lead isotope ratio analysis results showed three main ore sources: polymetallic deposits in the Nanling Mountains, the eastern Hubei-northern Jiangxi metallogenic belt and the western Henan Qinling-Dabie metallogenic belt. The extensive source of minerals reflects the frequent trade of metal resources between Yue people and the Chu state, which is not only the economic basis for the close relationship between Hunan Yue people and Chu State but also an important driving force for the southward expansion of the Chu state and national integration in Hunan.