bluuebunny/crossref_metadata_2025_split huggingface dataset:
Genetic polymorphism in European and African sheep breeds reared in Hungary based on 48 SNPs associated with resistance to gastrointestinal parasite infection using KASP-PCR technique
Authors: Astuti Putri Kusuma , Gavojdian Dinu , Ilie Daniela Elena , Wanjala George , Monori István , Bagi Zoltán , Kusza Szilvia 
Published: May 2023

AbstractThis pilot study used an alternative and economically efficient technique, the Kompetitive Allele-Specific Polymerase Chain Reaction (KASP-PCR) to examine 48 SNPs from 11 parasite-resistance genes found on 8 chromosomes in 110 animals from five sheep breeds reared in Hungary; Hungarian Tsigai, White Dorper, Dorper, Ile de France, and Hungarian Merino. Allele and genotype frequencies, fixation index, observed heterozygosity, expected heterozygosity, F statistic, and their relationship with the Hardy–Weinberg equilibrium (WHE) and the polymorphic information content (PIC) were determined, followed by principal component analysis (PCA). As much as 32 SNPs out of the 48 initially studied were successfully genotyped. A total of 9 SNPs, 4 SNPs in TLR5, 1 SNP in TLR8, and 4 SNPs in TLR2 genes, were polymorphic. The variable genotype and allele frequency of the TLRs gene indicated genetic variability among the studied sheep breeds, with the Hungarian Merino exhibiting the most polymorphisms, while Dorper was the population with the most SNPs departing from the HWE. According to the PIC value, the rs430457884-TLR2, rs55631273-TLR2, and rs416833129-TLR5 were found to be informative in detecting polymorphisms among individuals within the populations, whereas the rs429546187-TLR5 and rs424975389-TLR5 were found to have a significant influence in clustering the population studied. This study reported a moderate level of genetic variability and that a low to moderate within-breed diversity was maintained in the studied populations.
A tale of two events: Arctic rain-on-snow meteorological drivers
Authors: Voveris Jessica , Serreze Mark 
Published: May 2023

AbstractArctic rain-on-snow (ROS) events can have significant impacts on Arctic wildlife and socio-economic systems. This study addresses the meteorology of two different Arctic ROS events. The first, occurring near Nuuk, Greenland, generated significant impacts, including slush avalanches. The second, less severe, event occurred within the community of Iqaluit, Nunavut, Canada. This research utilizes atmospheric reanalysis, automated surface observation station data and atmospheric soundings to determine the meteorological conditions driving these events and the differences between each case. In both cases, atmospheric blocking played a leading role in ROS initiation, with atmospheric rivers – narrow bands of high water vapor transport, typically originating from the tropics and subtropics – having both direct and indirect effects. Cyclone-induced low-level jets and resultant ‘warm noses’ of higher air temperatures and moisture transport were other key features in ROS generation. To our knowledge, our study is the first to visualize how the varying strength and manifestation of these coupled features contribute to differences in the severity of Arctic ROS events. The meteorological drivers identified here find support from other studies on Arctic ROS events and are similar to weather features associated with Arctic precipitation events of extreme magnitude.
Antitrust as a Guardrail for Socially Responsible Neurotechnology Design
Authors: Nadler Roland 
Published: May 2023

ABSTRACT:The neurotechnology sector is likely to develop under pressure towards commercialized, nonmedical products and may also undergo market consolidation. This possibility raises ethical, social, and policy concerns about the future responsibility of neurotechnology innovators and companies for high-consequence design decisions. Present-day internet technology firms furnish an instructive example of the problems that arise when providers of communicative technologies become too big for accountability. As a guardrail against the emergence of similar problems, concerned neurotechnologists may wish to draw inspiration from antitrust law and direct efforts, where appropriate, against undue consolidation in the commercial neurotechnology market.
Mapping the Landscape of Equitable Access to Advanced Neurotechnologies in Canada
Authors: Harding Louise , McFarlane Jacob , Honey Christopher R. , McDonald Patrick J. , Illes Judy 
Published: May 2023

ABSTRACT:Geographic, social, political, and economic factors shape access to advanced neurotechnologies, yet little previous research has explored the barriers, enablers, and areas of opportunity for equitable and meaningful access for diverse patient communities across Canada. We applied a mixed-mode approach involving semi-structured interviews and rating scale questions to consult with 24 medical experts who are involved in the care of patients who undergo functional neurosurgery targeting the brain. Seven major themes emerged from the qualitative analysis: Health care system, Neurotechnology features, Patient demographics, Target condition features, Ethics, Upstream barriers and enablers, and Areas of opportunity. Descriptive statistics of the Likert-scale responses suggest that interviewees perceive a disparity between the imperative of access to advanced neurotechnologies for people living in rural and remote areas and the likelihood of achieving such access. The results depict a complex picture of access to functional neurosurgery in Canada with pockets of excellence and a motivation to improve the availability of care for vulnerable populations through the expansion of distributed care models, improved health care system efficiencies, increasing funding and support for patient travel, and increasing awareness about and advocacy for advanced neurotechnologies.
Concurrent dose-finding of a novel cancer drug with and without a second agent
Authors: Wages Nolan A. , Saleh Ramy R. , Braun Thomas M. 
Published: May 2023

Abstract

Introduction:
More complex research questions are being posed in early-phase oncology clinical trials, necessitating design strategies tailored to contemporary study objectives. This paper describes the proposed design of a Phase I trial concurrently evaluating the safety of a hematopoietic progenitor kinase-1 inhibitor (Agent A) as a single agent and in combination with an anti-PD-1 agent in patients with advanced malignancies. The study’s primary objective was to concurrently determine the maximum tolerated dose (MTD) of Agent A with and without anti-PD-1 therapy among seven possible study dose levels.

Methods:
Our solution to this challenge was to apply a continual reassessment method shift model to meet the research objectives of the study.

Results:
The application of this method is described herein, and a simulation study of the design’s operating characteristics is conducted. This work was developed through collaboration and mentoring between the authors at the American Association for Cancer Research (AACR) and the American Society of Clinical Oncology (ASCO) annual AACR/ASCO Methods in Clinical Cancer Research Workshop.

Conclusions:
The aim of this manuscript is to highlight examples of novel design applications as a means of augmenting the implementation of innovative designs in the future and to demonstrate the flexibility of adaptive designs in satisfying modern design conditions. Although the design is presented using an investigation of Agent A with and without anti-PD-1 therapy as an illustrative example, the approach described is not specific to these agents and could be applied to other concurrent monotherapy and combination therapy studies with well-defined binary safety endpoints.
Dealing with the Facts of Life: The Management of Intra-Party Factionalism in the Iberian Radical Left Parties
Authors: Lourenço Pedro , Conceição Tiago , Jalali Carlos 
Published: May 2023

AbstractFactionalism is an important element in parties' internal life, affecting their policies, strategies, electoral performance and even survival. Yet little is known about how parties manage factionalism. This study examines how radical left parties (RLPs) manage factionalism, drawing on a comparative case study of four RLPs – the Spanish Podemos and United Left (IU), and the Portuguese Left Bloc (BE) and Communist Party (PCP) – from 2010 to 2019. Drawing on original data collection, we find that parties adopt both formal and informal mechanisms to address factionalism. However, their approaches differ significantly. We identify two main approaches towards factionalism: apermissiveapproach, which allows internal pluralism, in the BE and IU; and aprohibitionistapproach, which actively fights factionalism, in Podemos and the PCP, withcompetitiveprohibitionism in the former andpre-emptiveprohibitionism in the latter. These approaches strongly correlate with parties' origins and political orientation, but neither fully prevents intra-party conflict or splintering.
SEMI-EQUATIONAL THEORIES
Authors: CHERNIKOV ARTEM , MENNEN ALEX 
Published: May 2023

AbstractWe introduce and study (weakly) semi-equational theories, generalizing equationality in stable theories (in the sense of Srour) to the NIP context. In particular, we establish a connection to distality via one-sided strong honest definitions; demonstrate that certain trees are semi-equational, while algebraically closed valued fields are not weakly semi-equational; and obtain a general criterion for weak semi-equationality of an expansion of a distal structure by a new predicate.
Ancient Human Mitogenome of the Beagle Channel (Tierra del Fuego): An Argentine Collaborative Project
Authors: Arencibia Valeria , Muñoz Hidalgo Marianne , Crespo Cristian , Maldonado Lucas , Lichtenstein Gabriel , Kamenetzky Laura , Vera Pablo , Zangrando Atilio F. , Tessone Augusto , Avena Sergio , Lia Verónica , Puebla Andrea , Dejean Cristina 
Published: May 2023

AbstractThe increasing use of massively parallel sequencing in the study of current and ancient human populations has enabled new approaches to bioanthropological and archaeological issues; however, its application to archaeological samples requires the use of technologies that are not easily accessible outside US and European research centers. To obtain an ancient mitogenome in Argentina, several institutions collaborated to apply massively parallel sequencing and bioinformatic methodologies on an enriched ancient DNA library of an individual from the Beagle Channel (dated 1504 ± 46 years BP), a region of particular interest for this line of inquiry. Phylogenetic reconstruction showed a close relationship with a Yamana from Navarino Island and an individual from Hoste Island (Chilean Antarctic Province): the three shared an ancestor who lived between 203 and 4,439 years ago. These three have mutations reported only for current and ancient individuals from the Beagle Channel, and their relationship with the rest of the D1g sub-haplogroups is unclear. The results obtained here are consistent with the reduction of mobility in the Fuegian archipelago around 4500 years BP that has been proposed based on archaeological evidence.
Wheat seed demand assessment assisted by genotyping in Ethiopia
Authors: Habte Endeshaw , Yirga Chilot , Jaleta Moti , Tesfaye Kindie , Bekele Adam , Erenstein Olaf 
Published: May 2023

Summary
This study examines the extent to which wheat varieties supplied by the formal seed system align with the varieties demanded and used by farmers in Ethiopia. The framework of stated and revealed preferences drawn from the consumer preference theory is used to analyze farmer demand for different wheat varieties. We used official data from the formal seed sector and representative survey data from wheat farm households in Ethiopia. The survey data allow to contrast the farmer reported varietal use with genotyping by sequencing (also known as DNA fingerprinting). Farmers’ reliance on informal seed sources and own saved seed, among others, contributes to the misidentification of the varieties they grow. Consequently, farmers are likely to misinform the formal seed demand assessment leading to either an over- or underestimation of actual seed demand for specific wheat varieties. Genotyping by sequencing, as opposed to farmer reports, established the persistence of old varieties. This also implies vulnerability of wheat production to disease dynamics depending on the longevity of disease resistance by the variety in use. Apart from narrowing the gap between the actual and stated demand and ensuring timely replacement of wheat varieties, genotyping-assisted estimates can save seed carry-over cost. Genotyping by sequencing is increasingly used as the new benchmark and gold standard for identifying and tracking the adoption of crop varieties. The technique has potential to enhance the performance of the seed sector through effective planning that can optimize resource commitments and accelerate the rate of varietal replacement.
Joint Inversion of Geodetic and Strong Motion Data for the 2012, Mw 6.1–6.0, May 20th and May 29th, Northern Italy Earthquakes: Source Models and Seismotectonic Interpretation
Authors: Improta L. , Cirella A. , Pezzo G. , Molinari I. , Piatanesi A. 
Published: May 2023

AbstractWe present the first rupture models of the two mainshocks of the 2012 northern Italy sequence, determined by jointly inverting seismic and geodetic data. We aim at providing new insights into the mainshocks for which contrasting seismotectonic interpretations are proposed in literature. Sources' geometric parameters were constrained by seismic reflection profiles, 3‐D relocations and focal mechanisms of mainshocks/aftershocks. Site‐specific velocity profiles were used to model accelerograms affected by strong propagation effects related to the Po basin. Our source models differ significantly from previous ones relying on either seismic or geodetic data. Their comparison against geological sections and aftershock distribution provides new insights about the ruptured thrust faults. The May 20th Mw6.1 mainshock activated the Middle Ferrara thrust‐ramp dipping ∼45° SSW‐wards, breaking a main eastern slip patch 4–15 km deep in Mesozoic carbonates (maximum slip 0.7–0.8 m) and Paleozoic‐Triassic basement rocks, and a small western patch in the basement. The May 29th Mw6.0 mainshock featured two separated asperities along the Mirandola thrust‐ramp dipping ∼42° S‐wards: an eastern asperity 4–15 km deep in Mesozoic carbonates and basement rocks (maximum slip 0.7 m) and a deeper western one (7–16 km depth) mainly in the basement (slip peak 0.8 m). On‐fault aftershocks were concentrated within the basement and Mesozoic carbonates, devoiding high‐slip zones. Slip and aftershock distribution was controlled by the rheological transition between Mesozoic carbonates and Cenozoic sediments. Unlike previous thin‐skinned tectonic interpretations, our results point to a complex rupture process along moderately dipping (40°–45°) thrust‐ramps deeply rooted into the Paleozoic crystalline basement.
A Tropical Cocktail of Organic Matter Sources: Variability in Supraglacial and Glacier Outflow Dissolved Organic Matter Composition and Age Across the Ecuadorian Andes
Authors: Holt Amy D. , Kellerman Anne M. , Battin Tom. I. , McKenna Amy M. , Hood Eran , Andino Patricio , Crespo‐Pérez Verónica , Peter Hannes , Schön Martina , De Staercke Vincent , Styllas Michail , Tolosano Matteo , Spencer Robert G. M. 
Published: May 2023

AbstractThe biogeochemistry of rapidly retreating Andean glaciers is poorly understood, and Ecuadorian glacier dissolved organic matter (DOM) composition is unknown. This study examined molecular composition and carbon isotopes of DOM from supraglacial and outflow streams (n = 5 and 14, respectively) across five ice capped volcanoes in Ecuador. Compositional metrics were paired with streamwater isotope analyses (δ18O) to assess if outflow DOM composition was associated with regional precipitation gradients and thus an atmospheric origin of glacier DOM. Ecuadorian glacier outflows exported ancient, biolabile dissolved organic carbon (DOC), and DOM contained a high relative abundance (RA) of aliphatic and peptide‐like compounds (≥27%RA). Outflows were consistently more depleted in Δ14C‐DOC (i.e., older) compared to supraglacial streams (mean −195.2 and −61.3‰ respectively), perhaps due to integration of spatially heterogenous and variably aged DOM pools across the supraglacial environment, or incorporation of aged subglacial OM as runoff was routed to the outflow. Across Ecuador, Δ14C‐DOC enrichment was associated with decreased aromaticity of DOM, due to increased contributions of organic matter (OM) from microbial processes or atmospheric deposition of recently fixed and subsequently degraded OM (e.g., biomass burning byproducts). There was a regional gradient between glacier outflow DOM composition and streamwater δ18O, suggesting covariation between regional precipitation gradients and the DOM exported from glacier outflows. Ultimately, this highlights that atmospheric deposition may exert a control on glacier outflow DOM composition, suggesting regional air circulation patterns and precipitation sources in part determine the origins and quality of OM exported from glacier environments.
Thank You to Our 2022 Peer Reviewers
Authors: Griffies Stephen M. , Fan Jiwen , MacBean Natasha , Schneider Tapio 
Published: May 2023

AbstractThe editors of Journal of Advances in Modeling Earth Systems thank the 702 reviewers who provided 1362 reviews during 2022. Their hard work and insights, typically done anonymously, benefits authors, readers, and the broader science community.
Design improvement of the conversion mechanism from balloon inflation to bending motion for inflatable film actuators
Authors: Hori Y. , Konishi S. 
Published: May 2023

AbstractVarious soft actuators have been investigated to overcome the drawbacks of conventional solid machines and explore the applications of soft robotics. In particular, and because they are expected to be applicable in minimally invasive medicine because of their safety, soft inflatable microactuators using an actuation conversion mechanism from balloon inflation to bending motion have been proposed for high-output bending motion. These microactuators could be applied to create an operation space by safely moving organs and tissues; however, the conversion efficiency could be further improved. This study aimed to improve conversion efficiency by investigating the design of the conversion mechanism. The contact conditions between the inflated balloon and conversion film were examined to improve the contact area for force transmission, with the contact area dependent on the length of the contact arc between the balloon and force conversion mechanism and on the amount of balloon deformation. In addition, surface contact friction between the balloon and film, which affects actuator performance, was also investigated. The generated force of the improved device is 1.21 N at 80 kPa when it bends 10 mm, which is 2.2 times the generated force of the previous design. This improved soft inflatable microactuator is expected to assist in performing operations in a limited space, such as in endoscopic or laparoscopic operations.
Glioma immunotherapy enhancement and CD8-specific sialic acid cleavage by isocitrate dehydrogenase (IDH)-1
Authors: Cordner Ryan , Jhun Michelle , Panwar Akanksha , Wang HongQiang , Gull Nicole , Murali Ramachandran , McAbee Joseph H. , Mardiros Armen , Sanchez-Takei Akane , Mazer Mia W. , Fan Xuemo , Jouanneau Emmanuel , Yu John S. , Black Keith L. , Wheeler Christopher J. 
Published: May 2023

AbstractThe promise of adaptive cancer immunotherapy in treating highly malignant tumors such as glioblastoma multiforme (GBM) can only be realized through expanding its benefits to more patients. Alleviating various modes of immune suppression has so far failed to achieve such expansion, but exploiting endogenous immune enhancers among mutated cancer genes could represent a more direct approach to immunotherapy improvement. We found that Isocitrate Dehydrogenase-1 (IDH1), which is commonly mutated in gliomas, enhances glioma vaccine efficacy in mice and discerns long from short survivors after vaccine therapy in GBM patients. Extracellular IDH1 directly enhanced T cell responses to multiple tumor antigens, and prolonged experimental glioma cell lysis. Moreover, IDH1 specifically bound to and exhibited sialidase activity against CD8. By contrast, mutant IDH1R132H lacked sialidase activity, delayed killing in glioma cells, and decreased host survival after immunotherapy. Overall, our findings identify IDH1 as an immunotherapeutic enhancer that mediates the known T cell-enhancing reaction of CD8 desialylation. This uncovers a new axis for immunotherapeutic improvement in GBM and other cancers, reveals novel physiological and molecular functions of IDH1, and hints at an unexpectedly direct link between lytic T cell function and metabolic activity in target cells.
Lysogenic bacteriophages encoding arsenic resistance determinants promote bacterial community adaptation to arsenic toxicity
Authors: Tang Xiang , Zhong Linrui , Tang Lin , Fan Changzheng , Zhang Baowei , Wang Mier , Dong Haoran , Zhou Chengyun , Rensing Christopher , Zhou Shungui , Zeng Guangming 
Published: May 2023

Abstract
Emerging evidence from genomics gives us a glimpse into the potential contribution of lysogenic bacteriophages (phages) to the environmental adaptability of their hosts. However, it is challenging to quantify this kind of contribution due to the lack of appropriate genetic markers and the associated controllable environmental factors. Here, based on the unique transformable nature of arsenic (the controllable environmental factor), a series of flooding microcosms was established to investigate the contribution of arsM-bearing lysogenic phages to their hosts’ adaptation to trivalent arsenic [As(III)] toxicity, where arsM is the marker gene associated with microbial As(III) detoxification. In the 15-day flooding period, the concentration of As(III) was significantly increased, and this elevated As(III) toxicity visibly inhibited the bacterial population, but the latter quickly adapted to As(III) toxicity. During the flooding period, some lysogenic phages re-infected new hosts after an early burst, while others persistently followed the productive cycle (i.e., lytic cycle). The unique phage-host interplay contributed to the rapid spread of arsM among soil microbiota, enabling the quick recovery of the bacterial community. Moreover, the higher abundance of arsM imparted a greater arsenic methylation capability to soil microbiota. Collectively, this study provides experimental evidence for lysogenic phages assisting their hosts in adapting to an extreme environment, which highlights the ecological perspectives on lysogenic phage-host mutualism.