bluuebunny/crossref_metadata_2025_split huggingface dataset:
A Heuristic Autonomous Exploration Method Based on Environmental Information Gain During Quadrotor Flight
Authors: Zhang Tong , Yu Jiajie , Li Jiaqi , Pang Minghui 
Published: February 2023

Abstract
Autonomous exploration is a widely studied fundamental application in the field of quadrotors, which requires them to automatically explore unknown space to obtain complete information about the environment. The frontier-based method, one of the representative works on autonomous exploration, drives autonomous determination by the definition of frontier information so that complete information about the environment is available to the quadrotor. However, existing frontier-based methods are able to accomplish the task but still suffer from inefficient exploration, and how to improve the efficiency of autonomous exploration is the focus of research nowadays. Slow frontier generation affecting real-time viewpoint determination and insufficient determination methods affecting the quality of viewpoints are typical of these problems. Therefore, to overcome the aforementioned problems, this paper proposes a two-level viewpoint determination method for frontier-based autonomous exploration. Firstly, a sampling-based frontier detection method is presented for faster frontier generation, improving the immediacy of environmental representation compared to traditional traversal-based methods. Secondly, we consider the access to environmental information during flight for the first time, and design an innovative heuristic evaluation function to decide on high quality viewpoint as the next local navigation target in each exploration iteration. Extensive benchmark and real-world tests are conducted to validate our method. The results confirm that our method optimizes the frontier search time by 85%, the exploration time by around 20–30%, and the exploration path by 25–35%.
Lycium barbarum polysaccharide promotes the immunoprotective effects of recombinant Lactobacillus plantarum vaccine expressing Trichinella spiralis cathepsin F-like protease 1 gene
Authors: Lu Huinan , Wang Yue , Zhang Tongxuan , Quan Yu , Zhu Zhiyu , Xue Ying , Pan Tianxu , Huang Haibin , Shi Chunwei , Yang Wentao , Jiang Yanlong , Wang Jianzhong , Zeng Yan , Cao Xin , Wang Chunfeng , Wang Nan , Yang Guilian 
Published: February 2023

Abstract
Trichinellosis caused by Trichinella spiralis (T. spiralis) is a zoonotic disease that seriously endangers human health. At present, vaccines that used to prevent trichinellosis was an effective way, but the production of antibody levels and immunogenicity is weakness. Adjuvants has been studied to elevate the antibody levels and immunogenicity of the vaccines. Therefore, it is urgent need to develop an effective adjuvant for the T. spiralis vaccine. The recent studies have indicated that traditional Chinese medicine polysaccharides as adjuvants can play a promote role in vaccine and it is a low-toxicity, and biodegradable compound. In this study, BALB/c mice were orally inoculated with recombinant Lactobacillus plantarum (L. plantarum) vaccine expressing T. spiralis cathepsin F-like protease 1 gene (rTs-CPF1), and it was administered thrice in the same dose at 10 days intervals. Meanwhile, Lycium barbarum polysaccharide (LBP) was orally administration until to 37 days. Mice were infected with 350 T. spiralis muscle larvae (ML) at 37 days after the first immunization. The results showed that LBP enhanced specific IgG and sIgA antibody levels against T. spiralis CPF1 protein in mice immunized with L. plantarum rTs-CPF1 + LBP group compared with L. plantarum rTs-CPF1 group. Moreover, LBP improved the expression levels of IFN-γ and IL-4, and the numbers of intestinal and intramuscular worm burden was significantly decreased in L. plantarum rTs-CPF1 + LBP group compared to L. plantarum rTs-CPF1 group. The reduction rate of adult worm and ML burden reached 47.31% and 68.88% in L. plantarum rTs-CPF1 + LBP group, respectively. In conclusion, LBP have a promoting role on the immunoprotective effects of T. spiralis vaccine and it may be considered as a novel adjuvant against parasitic vaccine.
In Vitro Model stimulated by exposure to single and combined stress factors for Necrotizing Enterocolitis Running title: In Vitro Model stimulated by single and combined factors for NEC
Authors: gao chuchu , Feng Zongtai , Wang Lixia , Yang Zuming , Wang Sannan , Yu Shenglin 
Published: February 2023

Abstract
Purpose
Necrotizing enterocolitis (NEC) is a severe gastrointestinal disease in neonates, with high death rate. The pathogenesis of NEC is particularly complex, mainly involving inflammation and hypoxic damage. In vitro cell model is an indispensable tool to study the pathogenesis of NEC. This study explored the effects of different stress factors on intestinal injury in vitro.
Methods
IEC-6 cells were stimulated by exposure to different stressors, including lipopolysaccharide (LPS), cobalt chloride (CoCl2), and a combination of both. Cell viability was detected by CCK-8 assay. The expression of inflammatory cytokines (IL-6 and TNFα) at the gene and protein levels were measured by quantitative reverse transcription-polymerase chain reaction (qRT-PCR) and enzyme-linked immune-sorbent assay (ELISA). While the expression of tight junction proteins (Claudin-1 and zonula occludens [ZO]-1) were evaluated by qRT-PCR and western blotting, respectively.
Results
The decrease in IEC-6 cell viability was observed after stimulation by CoCl2 alone or in combination with LPS, but not after stimulation with LPS alone. The expression of IL-6 and TNFα increased in each group, especially in the combined stimulation group. After stimulation with CoCl2 alone or in combination with LPS, a decrease in Claudin-1 was observed, but an increase was detected after stimulation with LPS alone. ZO-1 decreased in both mRNA and protein levels after combined stimulation.
Conclusion
The combined stimulation of CoCl2 and LPS on IEC-6 cells could simultaneously induce severe inflammation and barrier damage, which may better simulate the pathological process of NEC. Further research is needed to determine whether this in vitro model can be used to study the pathogenesis of NEC.
Limited BA.5 neutralization by pre-exposure prophylaxis with tixagevimab/cilgavimab in CAR-T treated people
Authors: Vergori Alessandra , Matusali Giulia , Metafuni Elisabetta , Bordi Licia , Borrelli Paola , Loiacono Laura , Pansini Ilaria , Sorà Federica , Mazzotta Valentina , Paulicelli Jessica , Girardi Enrico , Cingolani Antonella , Vaia Francesco , Sica Simona , Maggi Fabrizio , Antinori Andrea 
Published: February 2023

Abstract
CAR T-cell recipients experience profound B-cell aplasia and hypogammaglobulinemia, being unable to mount any humoral response and at higher risk for severe COVID-19. Tixagevimab/cilgavimab has been approved for COVID-19 pre-exposure prophylaxis (PrEP) in immunocompromised people. 150/150 mg of tixagevimab/cilgavimab does not adequately neutralize against Omicron BA.5 and these results support recommendations on dose increase to 300/300 mg for prophylaxis in order to enhance effectiveness probability, until the European regulatory agency makes a decision on the usability of this compound as the FDA has already done
An Investigation on Routing Techniques and Devolopment of a Secured Routing Techniques for Improving Performance and Security of Manets
Authors: SARKAR SAPTARSHI KUMAR , GHOSH JAYEETA , BISWAS RAKTIMA , FAIZAN MD 
Published: February 2023

Abstract
In this part, the requirement of conviction similarly to the need of Energy over the existent Ad hoc On Demand Distance Vector protocol (AODV) has caused the development of the proposed set of rules termed as, “The Refined Trust and Energy primarily based totally Adhoc on Demand Distance Vector set of rules (ReTE-AODV)” that has been a prominent technique for winning conviction except Energy structured Ad hoc On Demand Distance Vector algorithms (TEAODV) and the classical The projected set of rules shall effectively path packets from supply to vacation spot with a choice of dependable host via the shortest viable path that could devour lesser power for traversal of packets. Clustering Algorithms are extensively used to control the nodes correctly in huge scale networks. In order to limit this overhead a brand new set of rules referred to as B-CLUSTER is proposed in those researches which has a precept of electing heads at a time in a single election and feature the 2d CH as Reserved CH. When the primary CH leaves the Cluster, re-election isn't always required with-inside the proposed version because the reserve CH is said as the brand new CH. In summary, for any given wi-fi community, new routing and safety set of rules are proposed on this studies paintings for infrastructure primarily based totally and infrastructure much less networks. The ReTE-AODV and B-CLUSTER algorithms are proposed on this studies paintings are beneficial for secured routing in infrastructure much less.
Community VAT implementation în the Romanian tax system
Authors: Tilea Doina Maria , Nicolau Irina Ana , Dinu Ana-Maria 
Published: February 2023

Abstract
One Stop Shop became operational from July 1st, 2021. There are three types of regime: Union States regime, Non-Union States regime and Import regime. All distance sales of services and intra-Community sales of goods are now covered by the States of the Union regime.
JEL codes: M41, E62, H32
Binding analysis of horse and bovine ferritins using beads immobilized with alpha-casein-: detection of ferritin binding or nonbinding with heme using alpha-casein-immobilized beads
Authors: Hasegawa Ryo , Takahashi Ayana , Yoshikawa Yasunaga , Orino Koichi 
Published: February 2023

Abstract
Ferritin, iron storage protein, binds heme on its surface although it’s physiological role has not been elucidated yet. Firstly, this study analyzed the bindings of bovine and horse spleen ferritins to beads immobilized with α-casein (CasB) with the heme-mediated ferritin-binding capacity of α-casein (Cas). These spleen ferritins were added to solutions containing CasB, and the supernatant and beads obtained by centrifugation was subjected to polyacrylamide gel electrophoresis (SDS-PAGE or Native-PAGE) to detect ferritin. In case of horse spleen ferritin, CasB partly bound ferritin, and CasB-bound ferritin was detected only in the pelleted beads before the second addition of CasB to the first supernatant obtained before washing the beads, while the amount of ferritin detected in the first and second supernatants was similar. After addition of bovine spleen ferritin to CasB, some ferritin was also detected in the pelleted beads as CasB-binding protein, and the other ferritin was also detected in the supernatant as ferritin with no heme. On the other hand, the serum ferritin in fetal bovine serum showed little binding in the binding with CasB. These results suggest the following: CasB selectively detects heme-binding ferritin in tissue and serum ferritin, circulating ferritin contains little heme, and ferritin is intracellularly compartmented in heme catabolism as well as protection against its oxidative stress.
Insights of the degradation of plastics PET and PE type under composting conditions using coffee pulp as substrate
Authors: Dussán Mariana , García Andrés A. , López-Gonzalez Mar , Ruiz Christhy V. 
Published: February 2023

Abstract
The indiscriminate use, the alarming accumulation of plastic, and its subsequent poor well-disposal and management have generated the current environmental problem. As an environmentally friendly and effective strategy, composting processes arise like a method for waste treatment. This study explored the degradation of polyethylene terephthalate (PET) and low-density polyethylene (PE) plastic types under composting conditions for 90 days using coffee pulp as substrate into the compost. The degradation was estimated from weight variation over time, the thermal stability of the PET and PE samples was carried out using thermogravimetric analysis (TGA/DTA) whereas the chemical and morphological changes on the samples were assessed by FT-IR and SEM. The bacterial communities of composting were also investigated by 16S rRNA gene clone analysis. The results showed a partial degradation of PET and PE samples under composting conditions for the period of time considered. However, a strong adhesion effect was observed for PE samples which give place to a variation on the thermal stability of the samples. 16S RNA gene clone analysis showed that Sphingobacterium was the dominant bacteria, which is not related with the degradation of plastic material on composting. This result could be related with a reduced degradation during the composting treatment.
Developing research skills in medical students online using an active research study
Authors: Asghar Aziz U R , Aksoy Murat , Graham Alison I , Baseler Heidi A 
Published: February 2023

Abstract
Developing research skills and scholarship are key components of medical education. The COVID-19 pandemic necessitated that all teaching be delivered online. The purpose of our study was to use an online questionnaire to quantitatively evaluate medical student perspectives on development of their research and scholarship skills, teaching content and format, and tutor performance. In addition, we captured free text responses from both students and tutors on the positives and negatives of online teaching and suggested improvements. We also compared summative assessment marks for the online course with those obtained from previous in-person teaching sessions. Students were largely positive about most aspects of the online course utilising an active research study. Students agreed that they were able to acquire research skills, particularly related to data analysis, transferable skills, and giving scientific presentations. The assessment marks for the online course were comparable between the online course and the in-person laboratory teaching from previous years. Students enjoyed the convenience of online teaching and the availability of course resources, but least liked the lack of in-person interaction and laboratory training. Tutors enjoyed the collaborative aspects of online teaching, but least liked the lack of face-to-face interactions with students. Our study demonstrates that delivering online teaching, which involves students in active research, engages and motivates them to develop their research and scholarship skills. We recommend that educators consider incorporating a current research study in their undergraduate courses as this can enhance the student learning experience as well as the research project itself.
Chronic Non-toxic Megacolon Secondary to Diverticulitis: a case report
Authors: Donnarumma Biagio , Maio Rossella Di , Izzo Carmine , Pilone Vincenzo 
Published: February 2023

Abstract
Purpose: There are very few known cases of secondary non-toxic megacolon caused by complicated diverticulitis in literature and none of these have ever presented ab-extrinsic compression.
Methods: We present the case of a 65-year-old female patient with an unknown history of diverticulosis who presented to our hospital with a distended abdomen secondary to the obstruction of the sigmoid-rectum junction identified by an abdominal CT scan.
Results: Complicated diverticulitis created a fibrous string from the sigmoid-rectum junction to the homolateral ovary which caused an extrinsic compression leading to intestinal obstruction and non-toxic megacolon.
Conclusion: The purpose of this report is to share our experience and therapeutic choices to enrich the literature and prmote a better understanding of the disease.
Telomerase Expression Related with Poor Immune Response to HCV Core Antigen in Egyptian HCV Patients’ PBMCs
Authors: Ibrahim Iman Hassan , Ali Ola Sayed M. , El-Sahar Adel Ahmed , Elrefaei Mohamed , El-Sheikh Nabila 
Published: February 2023

Abstract
Hepatocellular carcinoma (HCC) is the most common primary malignant tumor of the liver. This study aimed to assess serum hTERT level and its relation to the progression of liver disease. Also, it aimed to assess the effect of HCV core protein on memory T-cells in HCV patients with or without HCC, and the correlation between memory cells phenotype and the progression of the disease in the same patients. hTERT level in serum was assessed through relative quantitative RT-PCR. Flow cytometric analysis was used to assess T-cells responsiveness (as IFN-γ secretion) before and after stimulation with HCV core protein, and memory CD8+ cells phenotype using several differentiation markers. hTERT was found to be increased in a stepwise manner upon comparing its level in controls, chronic hepatitis patients, cirrhotic patients, and HCC patients. T-cells showed a similar manner of stepwise decrease in response (decreased IFN-γ secretion) in HCC patients compared to HCV patients without HCC and controls. Also, late differentiated memory cells (CD8+ CD27- CD28- CD45RA+CCR7-) were depleted in HCC patients compared to HCV patients without HCC. In conclusion, these results suggest a negative correlation between hTERT and IFN-γ secretion by T- cells in HCV patients and that this relationship along with the depletion of late differentiated memory cells could help the progression of liver disease to HCC.
Establishment of a consensus protocol to explore the brain pathobiome in patients with mild cognitive impairment and Alzheimer's disease: Research outline and call for collaboration
Authors: Lathe* Richard , Schultek* Nikki M. , Balin Brian J. , Ehrlich Garth D. , Auber Lavinia Alberi , Perry George , Breitschwerdt Edward B. , Corry David B. , Doty Richard L. , Rissman Robert A. , Nara Peter L. , Itzhaki Ruth , Eimer William A. , Tanzi Rudolph E. , Collaborators Intracell Research Group Consortium 
Published: February 2023

Abstract
Microbial infections of the brain can lead to dementia, and for many decades microbial infections have been implicated in Alzheimer's disease (AD) pathology. However, a causal role for infection in AD remains contentious, and the lack of standardized detection methodologies has led to inconsistent detection/identification of microbes in AD brains. There is a need for a consensus methodology; the Alzheimer's Pathobiome Initiative aims to perform comparative molecular analyses of microbes in post-mortem brain versus cerebrospinal fluid (CSF), blood, olfactory neuroepithelium, oral/nasopharyngeal tissue, bronchoalveolar, urinary, and gut/stool samples. Diverse extraction methodologies, PCR and sequencing techniques, and bioinformatic tools will be evaluated, in addition to direct microbial culture and metabolomic techniques. The goal is to provide a roadmap for detecting infectious agents in patients with mild cognitive impairment or AD. Positive findings would then prompt tailoring of antimicrobial treatments that might attenuate or remit mounting clinical deficits in a subset of patients.
CELA1 Mediates Progressive Emphysema in Alpha-1 Antitrypsin Deficiency
Authors: Devine Andrew J. , Smith Noah J. , Joshi Rashika , Fan Qiang , Borchers Michael T. , Clair Geremy C. , Adkins Joshua N. , Varisco Brian M. 
Published: February 2023

Abstract

Chymotrypsin-like elastase 1 (CELA1) is a serine protease that is neutralized by α1-antitrypsin (AAT) and prevents emphysema in a murine antisense oligonucleotide model of AAT-deficient emphysema. Mice with genetic ablation of AAT do not have emphysema at baseline but develop emphysema with injury and aging. We tested the role of CELA1 in emphysema development in this genetic model of AAT-deficiency following tracheal lipopolysacharide (LPS), 8 months of cigarette smoke (CS) exposure, aging, and a low-dose tracheal porcine pancreatic elastase (LD-PPE) model. In this last model, we performed proteomic analysis to understand differences in lung protein composition. We were unable to show that AAT-/- mice developed more emphysema than wild type with LPS. In the LD-PPE model, AAT-/- mice developed progressive emphysema from which Cela1-/-&AAT-/- mice were protected. In the CS model, Cela1-/-&AAT-/- mice had worse emphysema than AAT-/-, and in the aging model, 72-75 week-old Cela1-/-&AAT-/- mice had less emphysema than AAT-/- mice. Proteomic analysis of AAT-/- vs. wildtype lungs in the LD-PPE model showed reduced amounts of AAT proteins and increased amounts of proteins related to Rho and Rac1 GTPases and protein oxidation. Similar analysis of Cela1-/-&AAT-/- vs. AAT-/- lungs showed differences in neutrophil degranulation, elastin fiber synthesis, and glutathione metabolism. Thus, Cela1 prevents post-injury emphysema progression in AAT-deficiency, but it has no effect and potentially worsens emphysema in response to chronic inflammation and injury. Prior to developing anti-CELA1 therapies for AAT-deficient emphysema, an understanding of why and how CS exacerbates emphysema in Cela1 deficiency is needed.
N-BEATS Perceiver: A Novel Approach for Robust Cryptocurrency Portfolio Forecasting
Authors: Sbrana Attilio , de Castro Paulo André Lima 
Published: February 2023

Abstract

Cryptocurrencies are well-known for their high volatility and unpredictability, posing a challenge for forecasting using traditional methods. To address this issue, we explore variations of the N-BEATS deep learning (DL) architecture by adding convolutional network layers, Transformer mechanisms, and the Mish activation function, and propose a novel approach for forecasting cryptocurrency portfolios. Our comprehensive evaluation demonstrates that our model variations outperform other DL and traditional forecasting methods in numerous evaluation metrics, making them powerful tools for predicting cryptocurrency prices and portfolios in the rapidly-evolving cryptocurrency market. Furthermore, our newly proposed N-BEATS Perceiver model, a Transformer-based N-BEATS variation, exhibits a robust risk profile with less downside compared to other models and performs exceptionally well when evaluated using the TOPSIS method across a wide range of portfolio evaluation parameters. These results underscore the potential of our approach and specifically highlight the N-BEATS Perceiver's potential for selecting portfolios and forecasting cryptocurrency prices, offering valuable insights into the development of more accurate and reliable models for cryptocurrency forecasting.
Single-cell RNA sequencing applications in abdominal aortic aneurysm: A mini review
Authors: Algabri Yousif A. 
Published: February 2023

Abstract
In the past decade, advances in single-cell RNA sequencing (scRNA-seq) technology have revolutionized biomedical research, enabling the characterization and analysis of the transcriptomes of single cells with remarkable precision and throughput. Specifically, scRNA-seq has assisted in the identification of novel or rare cell types, the analysis of single-cell trajectory formation and stem or progenitor cell development, and the single-cell resolution comparison of healthy and disease-related tissues. The generation of cell atlases of the mammalian heart and blood vessels, as well as the elucidation of mechanisms involved in cardiovascular development and stem or progenitor cell differentiation, demonstrate the importance of these applications to the advancement of cardiovascular research over the past decade. This review examines the applications of scRNAseq in AAA research and discusses the molecular pathways of cardiovascular development and diseases uncovered by scRNA-seq analysis that could be implemented in clinical practice.