bluuebunny/crossref_metadata_2025_split huggingface dataset:
Higher Fasting Pretransplant C-peptide Levels in Type 2 Diabetics Undergoing Simultaneous Pancreas-kidney Transplantation Are Associated With Posttransplant Pancreatic Graft Dysfunction
Authors: Parajuli Sandesh , Mandelbrot Didier , Aufhauser David , Kaufman Dixon , Odorico Jon 
Published: January 2023

Background.
Among selected patients with type 2 diabetes mellitus (T2DM), simultaneous pancreas and kidney (SPK) transplants can be an effective option. However, data are limited about outcomes in T2DM SPK recipients based on the pretransplant C-peptide levels.

Methods.
In this study, we reviewed all T2DM SPK recipients and categorized them based on the pretransplant fasting C-peptide levels into 3 groups: low (≤2 ng/mL), medium (>2–8 ng/mL), and high (>8 ng/mL). Several measures of graft failures (GFs), graft dysfunction, and composite outcomes were of interest.

Results.
There were a total of 76 SPK recipients (low, n = 14; medium, n = 47; high, n = 15). At the last follow-up, the low group did not reach any outcome; in contrast, 11 (23%) in the medium group and 5 (33%) in the high group reached the uncensored composite outcome; 6 (13%) in the medium group and 2 (13%) in the high group had GF; and 8 (17%) in the medium group and 4 (26.7%) in the high group reached the death-censored composite outcomes. In a fully adjusted model, each pretransplant C-peptide unit was not associated with an increased risk of the composite outcome, GF, or death-censored composite outcomes. However, in multivariate analysis with limited adjustment, pretransplant C-peptide was associated with the composite outcome (hazard ratio: 1.18, 95% confidence interval, 1.01-1.38; P = 0.03) and death-censored composite outcome (hazard ratio: 1.20; 95% confidence interval, 1.01-1.42; P = 0.03).

Conclusions.
Although limited by the small sample size, we found excellent outcomes among T2DM SPK recipients overall. However, higher levels of pretransplant C-peptide may be associated with inferior posttransplant outcomes that include graft dysfunction.
Melaminivora suipulveris sp. nov., isolated from pigpen dust
Authors: Won Miyoung , Heo Jun , Lee Daseul , Han Byeong-Hak , Hong Seung-Beom , Kwon Soon-Wo 
Published: January 2023

A bacterial strain designated SC2-9T was isolated from the dust collector of a pigpen located in Wanju-gun, Jeollabuk-do, Republic of Korea. Cells were strictly aerobic, Gram-stain-negative, flagellated and rod-shaped. The strain was catalase- and oxidase-positive, and grew optimally 28–30 °C, pH 8.0 and 0 % NaCl (w/v). Phylogenetic analysis based on 16S rRNA gene sequences showed 99.1 and 98.3 % similarities to

Melaminivora jejuensis

KBB12T and

Melaminivora alkalimesophila

CY1T, and revealing less than 97 % similarity to other validly named species. The genomic DNA G+C content of strain SC2-9T was 68.2 %. The orthologous average nucleotide identity and dDDH values of strain SC2-9T with the closest species

Melaminivora jejuensis

KCTC 32230T were 85.6 and 29.3 %, respectively. The polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, three unidentified aminolipids and one unidentified lipid. The major fatty acids (>10 %) were summed feature 3 (C16 : 1
ω6c and/or C16 : 1
ω7c), C16 : 0 and summed feature 8 (C18 : 1
ω6c and/ or C18 : 1
ω7c). The predominant isoprenoid quinone was ubiquinone-8. Based on phenotypic, chemotaxonomic and phylogenetic data, strain SC2-9T should be assigned as a novel species of the genus

Melaminivora

, for which the name Melaminivora suipulveris sp. nov. is proposed. The type strain is SC2-9T (=KACC 19310T=NBRC 113103T).
ABERRANT IMMUNE PROGRAMMING IN NEUTROPHILS IN CYSTIC FIBROSIS
Authors: Hu Yawen , Bojanowski Christine M. , Britto Clemente J. , Wellems Dianne , Song Kejing , Scull Callie , Jennings Scott , Li Jianxiong , Kolls Jay K. , Wang Guoshun 
Published: January 2023

ABSTRACTCystic fibrosis (CF) is a life-shortening genetic disorder, caused by mutations in the gene that encodes Cystic Fibrosis Transmembrane-conductance Regulator (CFTR), a cAMP-activated chloride and bicarbonate channel. Although multiple organ systems can be affected, CF lung disease claims the most morbidity and mortality due to chronic bacterial infection, persistent neutrophilic inflammation, and mucopurulent airway obstruction. Despite the clear predominance of neutrophils in these pathologies, how CFTR loss-of-function affects these cellsper seremains incompletely understood. Here, we report the profiling and comparing of transcriptional signatures of peripheral blood neutrophils from CF participants and healthy human controls (HC) at the single-cell level. Circulating CF neutrophils had an aberrant basal state with significantly higher scores for activation, chemotaxis, immune signaling, and pattern recognition, suggesting that CF neutrophils in blood are prematurely primed. Such an abnormal basal state was also observed in neutrophils derived from an F508del-CF HL-60 cell line, indicating an innate characteristic of the phenotype. LPS stimulation drastically shifted the transcriptional landscape of HC circulating neutrophils towards a robust immune response, however, CF neutrophils were immune-exhausted. Moreover, CF blood neutrophils differed significantly from CF sputum neutrophils in gene programming with respect to neutrophil activation and aging, as well as inflammatory signaling, highlighting additional environmental influences on the neutrophils in CF lungs. Taken together, loss of CFTR function has intrinsic effects on neutrophil immune programming that leads to premature priming and dysregulated response to challenge.
Shielding under endemic SARS-CoV-2 conditions is easier said than done: a model-based analysis
Authors: Stoddard Madison , Yuan Lin , Sarkar Sharanya , Mazewski Matthew , van Egeren Debra , Mangalaganesh Shruthi , Nolan Ryan P. , Rogers Michael S. , Hather Greg , White Laura F. , Chakravarty Arijit 
Published: January 2023

AbstractAs the COVID-19 pandemic continues unabated, many governments and public-health bodies worldwide have ceased to implement concerted measures for limiting viral spread, placing the onus instead on the individual. In this paper, we examine the feasibility of this proposition using an agent-based model to simulate the impact of individual shielding behaviors on reinfection frequency. We derive estimates of heterogeneity in immune protection from a population pharmacokinetic (pop PK) model of antibody kinetics following infection and variation in contact rate based on published estimates. Our results suggest that individuals seeking to opt out of adverse outcomes upon SARS-CoV-2 infection will find it challenging to do so, as large reductions in contact rate are required to reduce the risk of infection. Our findings suggest the importance of a multilayered strategy for those seeking to reduce the risk of infection. This work also suggests the importance of public health interventions such as universal masking in essential venues and air quality standards to ensure individual freedom of choice regarding COVID-19.
A Probabilistic Approach to Physician-Assisted Suicide
Authors: Nov Oded 
Published: January 2023

AbstractPhysician-Assisted Suicide (PAS) is considered by some patients who learn they are at risk for a cognitive decline owing to Alzheimer’s Disease. At the same time, the prospect of PAS may raise patients’ fear of imminent death. Can PAS be offered in a way that is preference-sensitive on one hand, and mitigates patients’ fear of imminent death on the other? A thought experiment of aProbabilistic Approach to PAS(Probabilistic PAS) is proposed here as a possible solution in jurisdictions where PAS is legal.Consider the following scenario: A patient diagnosed with Alzheimer’s Disease who is considering PAS might request that when he or she can no longer recognize their loved ones, their doctor will give them a lethal pill that has a 1/100 daily probability of being activated. As a result, after taking the pill, every day the patient will have a 1/100 probability of dying. The likelihood of the patient dying within a year from taking the pill is 97.4%, and within two years, it is 99.9%. As such, a Probabilistic PAS with which on any given day the probability of dying is low, can help patients avoid the fear of imminent death – which traditional PAS entails – while respecting their preference to end their lives.To examine the potential reception of a Probabilistic PAS, a survey was administered to a nationally-representative sample of US residents, using Prolific, a research participant recruitment platform. 499 participants were presented with a short description of a patient who was diagnosed with Alzheimer’s Disease, is writing an advance directive and is considering ways to end their life painlessly when they can no longer recognize their loved ones. Participants were asked about their own preferences in case they were to face a similar situation, and whether helping administer Probabilistic PAS would be ethical for the patient’s provider.498 participants responded to the question about their own preference. 73.5% indicated that they would choose one of the two PAS options. Among those, 9.8% preferred a Probabilistic PAS over traditional PAS. Men were more likely than women to favor Probabilistic PAS for themselves. 482 participants indicated which option would be most ethical for the patient’s provider to administer. 48.1% indicated one of the two PAS options as most ethical. Among those, 10.3% considered Probabilistic PAS to be more ethical than traditional PAS. Men were more likely than women to consider the provider administering Probabilistic PAS to be most ethical.A version of the Probabilistic PAS proposed here should be considered as a preference-sensitive option presented by healthcare providers to patients considering advance care planning in places where PAS is available.
Lost in space(s): multimodal neuroimaging of disorientation along the Alzheimer’s disease continuum
Authors: Peters-Founshtein Gregory , Gazit Lidor , Naveh Tahel , Domachevsky Liran , Korczyn Amos , Bernstine Hanna , Groshar David , Marshall Gad A. , Arzy Shahar 
Published: January 2023

AbstractOrientation is a fundamental cognitive faculty, allowing the behaving self to link his/her current state to their internal representations of the external world. Once exclusively linked to knowledge of the current place and present time, in recent years, the concept of orientation has evolved to include processing of social, temporal, and abstract relations. Concordantly with the growing focus on orientation, spatial disorientation has been increasingly recognized as a hallmark symptom of Alzheimer’s disease (AD). However, few studies have sought to explore disorientation along the AD continuum beyond the spatial domain.51 participants along the AD continuum performed an orientation task in the spatial, temporal and social domains. Under functional magnetic resonance imaging (fMRI), participants determined which of two familiar places/events/people is geographically/chronologically/socially closer to them, respectively. A series of analyses revealed disorientation along the AD-continuum to follow a three-way association between (1) orientation domain, (2) brain region, and (3) disease stage. Specifically, participants with MCI exhibited impaired spatio-temporal orientation and reduced task-evoked activity in temporoparietal regions, while participants with AD dementia exhibited impaired social orientation and reduced task-evoked activity in frontoparietal regions. Furthermore, these patterns of hypoactivation coincided with Default Mode Network (DMN) sub-networks, with spatio-temporal orientation activation overlapping DMN-C and social orientation with DMN-A. Finally, these patterns of disorientation-associated hypoactivations coincided with patterns of fluorodeoxyglucose (FDG) hypometabolism and cortical atrophy characteristic to AD-dementia.Taken together, our results suggest that AD may constitute a disorder of orientation, characterized by a biphasic process as (1) early spatio-temporal and (2) late social disorientation, concurrently manifesting in task-evoked and neurodegenerative changes in temporoparietal and parieto-frontal brain networks, respectively. We propose that a profile of disorientation across multiple domains offers a unique window into the progression of AD.
Identification and characterization of an HtrA sheddase produced byCoxiella burnetii
Authors: Osman Ikram Omar , Caputo Aurelia , Pinault Lucile , Mege Jean-Louis , Levasseur Anthony , Devaux Christian A. 
Published: January 2023

AbstractHaving previously shown that soluble E-cadherin (sE-cad) is found in sera of Q fever patients, and that infection of BeWo cells byC. burnetiileads to modulation of the E-cad/β-cat pathway, our purpose was to identify which sheddase(s) might catalyze the cleavage of E-cad. Here, we searched for a direct mechanism of cleavage initiated by the bacterium itself, assuming the possible synthesis of a sheddase encoded in the genome ofC. burnetiior an indirect mechanism based on the activation of a human sheddase. Using a straightforward bioinformatics approach to scan the complete genomes of four laboratory strains ofC. burnetii, we demonstrate thatC. burnetiiencodes a 451 amino acid sheddase (CbHtrA) belonging to the HtrA family and differently expressed according to the bacterial virulence. An artificial CbHtrA gene (CoxbHtrA) was expressed and the CoxbHtrA recombinant protein was found to have sheddase activity. We also found evidence that theC. burnetiiinfection triggers an over-induction of the human HuHtrA gene expression. Finally, we demonstrate that cleavage of E-cad by CoxbHtrA on THP-1-cells leads to an M2 polarization of the target cells and the induction of their secretion of IL-10, which ‘disarms’ the target cells and improvesC. burnetiireplication. Taken together these results demonstrate that the genome ofC.burnetiiencodes a functional HtrA sheddase and establish a link between the HtrA sheddase-induced cleavage of E-cad, the M2 polarization of the target cells and their secretion of IL-10, and the intracellular replication ofC. burnetii.
Preparation and Characterization of Inactivated Tick-Borne Encephalitis Virus Samples for Single Particle Imaging at European XFEL
Authors: Vorovitch Mikhail F. , Samygina Valeriya R , Pichkur Evgeny , Konarev Peter V , Peters Georgy , Khvatov Evgeny V , Ivanova Alla L , Tuchynskaya Ksenia K. , Konyushko Olga I. , Fedotov Anton Y. , Armeev Grigory , Shaytan Konstantin V , Maia Filipe R N C , Kovalchuk Mikhail V. , Osolodkin Dmitry I. , Ishmukhametov Aydar A. , Egorov Alexey M. 
Published: January 2023

AbstractX-ray imaging of virus particles at European XFEL could eventually allow solving their complete structure, potentially approaching resolution of other structural virology methods. To achieve this ambitious goal with today’s technologies, several mL of purified virus suspension containing at least 1012particles per mL are required. Such large amounts of concentrated suspension have never before been obtained for enveloped viruses. Tick-borne encephalitis virus (TBEV) represents an attractive model system for the development of enveloped virus purification and concentration protocols, given the availability of large amounts of inactivated virus material provided by vaccine manufacturing facilities. Here we present the development of a TBEV vaccine purification and concentration scheme combined with a quality control protocol allowing substantial amounts of highly concentrated non-aggregated suspension to be obtained. Preliminary single particle imaging experiments were performed for this sample at European XFEL, showing distinct diffraction patterns.
Supplier–supplier coopetition and buyer innovation: a perspective of learning and competitive tension within the focal buyer's supplier network
Authors: Guo Bin , Li Xi , Liu Tanfei , Wu Dong 
Published: January 2023

PurposeSupplier–supplier coopetition is vital to buyer innovation in reality. However, it has not received enough attention in prior research. Integrating the ability-motivation-opportunity framework of organizational learning perspective and the awareness-motivation-capability framework of competitive dynamics theory, this paper investigates the effect of supplier–supplier coopetition within supplier network on buyer innovation, as well as the contingent role of the relational attributes -- duration and tie strength dispersion of buyer–suppliers relationship at the supplier network level.Design/methodology/approachTesting this model on the secondary data of supply networks formed by 204 US listed buyer firms in SIC code 28, 35, 36 during 2008–2019, the authors utilize a fixed-effect regression model to investigate the relationship between supplier–supplier coopetition and the focal buyer's innovation.FindingsThe authors provide support for the positive influence of supplier–supplier cooperation on buyer innovation and an inverted U-shaped relationship between supplier–supplier competition and the focal buyer's innovation. The buyer–suppliers tie strength dispersion amplified the above two effects, and supplier–supplier cooperation mitigates the effect of supplier–supplier competition on the focal buyer's innovation.Originality/valueExtending the traditional dyadic view to a network-level view via linking the supplier–supplier dyad and the buyer–suppliers dyad, this paper contributes to a better understanding of supplier–supplier coopetition and its impact on buyer innovation with learning and competitive tension as the underlying explanations, and validates the contingent role of buyer–suppliers relational attributes.
Peer victimization and eating behaviors in college students: the mediating role of perceived stress
Authors: Rosen Lisa H. , Scott Shannon , Paulman Briana E. 
Published: January 2023

Purpose
This study aims to examine whether peer victimization predicted disordered eating behaviors during emerging adulthood, and if this relationship was mediated by perceived stress.

Design/methodology/approach
Participants included undergraduate females from a diverse university in the Southwestern USA who reported on their experiences of peer victimization, perceived stress and eating behaviors.

Findings
Mediation analysis revealed that perceived stress partially mediated the association between peer victimization and perceived stress. Peer victimization significantly predicted eating behaviors even after controlling for perceived stress. An exploratory analysis of the EAT-26 subscales was also conducted.

Research limitations/implications
The present study adds to the literature on peer victimization, eating behaviors and stress by allowing researchers to understand the complexity of these relationships within an emerging adulthood population. The present results can assist individuals working with this population in interventions to prevent instances of victimization, reduce stress and provide psychoeducation for eating disorders.

Originality/value
The present study adds to the literature on peer victimization, eating behaviors and stress by allowing researchers to understand the complexity of these relationships within a college population.
Reconfiguring the service system for resilience: lessons learned in the higher education context
Authors: Walletzký Leonard , Carrubbo Luca , Badr Nabil Georges , Dragoicea Monica , Toli Angeliki Maria , Badawi Salem 
Published: January 2023

Purpose
The COVID-19 pandemic started a new era in understanding the topic of resilience and adaptability. The human society has not faced such a widespread global challenge until now. This paper aims to address a context change influenced by the COVID-19 pandemic, using a case study in high education. While the character of the issues emerging is the same as in any other domain, in high education, the principles and consequences can be more directly studied and analyzed.

Design/methodology/approach
This paper describes a framework to evaluate how the context of the tertiary education service has been disrupted and the influence on the adherence of the students to the educational process, via primary quantitative data collection. This paper tackles the problem of distinguishing the change in context and context change and the possibility of system reconfiguration.

Findings
To properly face the evolving conditions induced by the pandemic, the education service system must be aligned to the imposed emergency situations, trying to “find” where the changes have emerged, i.e. what kind of reconfiguration is, whether it appears in the goals or in the service system itself. Furthermore, this study discusses how the findings can be valuable and applied to situations beyond the pandemic, in other cases of context disruption to highlight how general the service activities are within our reconfiguration approach.

Originality/value
From a theoretical point of view, this work is in line with main assumptions of system thinking, by confirming several insights of service systems’ behavior, even in a logic of B2B interactions (from the offer side); first in terms of openness and adaptation, in addition to readiness to change and – when and how – this change can occur. From a practical point of view, this paper’s contribution is directed toward achieving the more successful change management process, as reached together by motivated partners working hard for a common final goal. Realizing that the pandemic has brought a completely new context of education, managers should focus now on monitoring all aspects of the education business, not only directly affected projects and processes.
From values to valuing: an ethnographic approach to get a grip on the implicit disclosure of built heritage
Authors: De Ridder Roel , Van Gils Hanne , Timmermans Bert 
Published: January 2023

PurposeThe purpose of this paper is to map the process of (social) valuing by people encountering built heritage in their daily environments. Value-based approaches are not well researched and formalized in Flemish policy context. New questions and issues are emerging in relation to values-based heritage management and the (adaptive) reuse of heritage within a context of spatial development and urban renewal practices. This paper firstly focus on what factors influence the process of (social) valuing, secondly on the hybrid character of the process and finally at the conflicts between the values frames of the different actors. This way it also inquires the potentials of participatory design supporting alternative regimes of care.Design/methodology/approachWithin the research trajectory, the authors approached built heritage as a social construction and a social product, where there are as many stories as users. What heritage is and how heritage is dealt with, forms the basis of negotiation and valuation processes. An ethnographic approach was embarked on to get a grip on the socio-cultural significance of immovable property heritage in Flanders.FindingsThis paper describes the process of (social) valuing of by people encountering built heritage in their daily environments and offers an integrated conceptual framework for this kind of dynamic processes.Originality/valueNew questions and issues are emerging in relation to values-based heritage management and the (adaptive) reuse of heritage within a context of spatial development and urban renewal practices. This paper firstly focuses on what factors influence the process of (social) valuing, secondly on the hybrid character of the process and finally at the conflicts between the values frames of the different actors.