bluuebunny/crossref_metadata_2025_split huggingface dataset:
Be constantly different! How to manage influencer authenticity
Authors: Zniva Robert , Weitzl Wolfgang J. , Lindmoser Christina 
Published: January 2023

AbstractSocial media influencers are increasingly approached by marketers to advocate brands and products. This practice is commonly called ‘influencer marketing’. Influencers can take advantage of their reach and importance for consumers’ decision making by obtaining rewards from marketers. At the same time, consumers are increasingly aware of this practice. In this context, the perception of an influencers´ authenticity is key when it comes to his/her ability to persuade others. In this research, we shed light on the nature of the influencer authenticity construct, its boundaries as well as its relationships with brand-related variables responsible for consumers’ buying decisions. Using an experimental approach (n = 163), we demonstrate that especially influencers’ uniqueness and consistency increase their authenticity. Furthermore, our results show a strong impact of influencer authenticity on purchase intention, which is partially mediated via brand authenticity and brand attitude.
The complete ITS2 barcoding region for Strongylus vulgaris and Strongylus edentatus
Authors: Halvarsson Peter , Tydén Eva 
Published: January 2023

Abstract
Gastrointestinal nematode parasites are of major concern for horses, where Strongylus vulgaris is considered the most pathogenic among the Strongylus species. Diagnosis of S. vulgaris infections can be determined with next generation sequencing techniques, which are inherently dependent on reference sequences. The best marker for parasitic nematodes is internal transcribed spacer 2 (ITS2) and we provide the first complete ITS2 sequences from five morphologically identified S. vulgaris and additional sequences from two S. edentatus. These sequences have high similarity to already published partial sequences and amplicon sequence variants (ASV) based on next generation sequencing (NGS). The ITS2 sequences from S. vulgaris matched available partial ITS2 sequences and the full ASVs, whereas the S. edentatus sequence matched another complete sequence. We also compare Sanger sequencing and NGS methods and conclude that the ITS2 variation is better represented with NGS methods. Based on this, we recommend that further sequencing of morphologically identified specimens of various species should be performed with NGS cover the intraspecific variation in the ITS2.
Mammographic calcifications undergoing percutaneous biopsy: outcome in women with and without a personal history of breast cancer
Authors: Bertani Valeria , Berger Nicole , Eberhard Matthias , Lång Kristina , Urbani Martina , La Grassa Manuela , Balestreri Luca , Boss Andreas , Frauenfelder Thomas , Marcon Magda 
Published: January 2023

Abstract
Purpose
To compare the positive predictive values (PPVs) of BI-RADS categories used to assess pure mammographic calcifications in women with and without a previous history of breast cancer (PHBC).

Materials and methods
In this retrospective study, all consecutive pure mammographic calcifications (n = 320) undergoing a stereotactic biopsy between 2016 and 2018 were identified. Mammograms were evaluated in consensus by two radiologists according to BI-RADS and blinded to patient history and pathology results. Final pathologic results were used as the standard of reference. PPV of BI-RADS categories were compared between the two groups. Data were evaluated using standard statistics, Mann–Whitney U tests and Chi-square tests.

Results
Two hundred sixty-eight patients (274 lesions, median age 54 years, inter-quartile range, 50–65 years) with a PHBC (n = 46) and without a PHBC (n = 222) were included. Overall PPVs were the following: BI-RADS 2, 0% (0 of 56); BI-RADS 3, 9.1% (1 of 11); BI-RADS 4a, 16.2% (6 of 37); BI-RADS 4b, 37.5% (48 of 128); BI-RADS 4c, 47.3% (18 of 38) and BI-RADS 5, 100% (4 of 4). The PPV of BI-RADS categories was similar in patients with and without a PHBC (P = .715). Calcifications were more often malignant in patients with a PHBC older than 10 years (47.3%, 9 of 19) compared to 1–2 years (25%, 1 of 4), 2–5 years (20%, 2 of 10) and 5–10 years (0%, of 13) from the first breast cancer (P = .005).

Conclusion
PPV of mammographic calcifications is similar in women with or without PHBC when BI-RADS classification is strictly applied. A higher risk of malignancy was observed in patients with a PHBC longer than 10 years.
Microstructural, Mechanical and Wear Properties of Atmospheric Plasma-Sprayed and High-Velocity Oxy-Fuel AlCoCrFeNi Equiatomic High-Entropy Alloys (HEAs) Coatings
Authors: Silvello A. , Torres Diaz E. , Rúa Ramirez E. , Garcia Cano I. 
Published: January 2023

AbstractIn this investigation, atmospheric plasma spray (APS) and high-velocity oxy-fuel (HVOF) techniques were used to produce AlCoCrFeNi coatings. High-entropy alloys (HEAs), due to their mechanical, chemical, and physical properties are capturing the attention of the international scientific community. Starting feedstock powders were characterized in terms of size, phase, and size, and corrosion test in NaCl, ball on disk, rubber wheel, and jet erosion tests was carried out on the obtained coatings. The results of the tribological investigation show that in the case of APS coatings, corrosion and wear behavior depend on the microstructure phases of the coating, as well as the amount of oxides. In particular, the wear morphology of APS surfaces is characterized by brittle fracture, with the presence of pores, cracks, and grooves. For HVOF coatings, further investigations on process parameters are needed because of the poor adhesion strength between the coating and the substrate. Anyway, the obtained corrosion resistance of HVOF coating is greater than that of the C steel substrate used to benchmark the results, and in addition, it ensures better performances in rubber wheel and jet erosion tests, but its wear resistance in the ball-on-disk test is worse because of the debris remaining in the wear track.
Sharp systolic inequalities for rotationally symmetric 2-orbifolds
Authors: Lange Christian , Soethe Tobias 
Published: January 2023

AbstractWe show that suitably defined systolic ratios are globally bounded from above on the space of rotationally symmetric spindle orbifolds and that the upper bound is attained precisely at so-called Besse metrics, i.e. Riemannian orbifold metrics all of whose geodesics are closed. The first type of systolic ratios that we consider are defined in terms of closed geodesics that lift to contractible loops on certain covers of the unit sphere bundle. The second type of systolic ratios are defined in terms of the kth shortest closed geodesic, where the number k depends on the underlying orbifold. Our results generalize a corresponding result of Abbondandolo, Bramham, Hryniewicz and Salomão for spheres of revolution, even in the manifold case. Moreover, they complement recent results by Abbondandolo, Mazzucchelli and the first named author on local systolic inequalities for Besse Reeb flows on closed 3-manifolds.
Creating interior support structures with Lightweight Voronoi Scaffold
Authors: Piros Attila , Trautmann Laura 
Published: January 2023

AbstractNowadays product designers have possibilities to design complex geometries, since for instance with additive manufacturing, there is less technological limits then before. However, besides that they have geometric freedom, it is essential to pay attention to engineering aspects, such as efficient material usage, stiffness and so on. This article is dealing with internal support structures and introduces a new lattice, called Lightweight Voronoi Scaffold. The scaffolds as 3-dimensional structures are well known in numerous fields of science. These structures provide mechanical stiffness for bones and place for biomolecules as well. The aim of this research was testing this new structure in case of complex geometry with multiaxial load case. Therefore, the arrangement of Voronoi scaffold is not regular, random sampling-based Monte Carlo method was applied in order to provide proper distribution of generation of geometric instances. Although the random point seed generates a high number of improper geometries, the remaining ones always include notable solutions. Lightweight Voronoi Scaffold was compared to some common regular beam lattices, and results shown that Lightweight Voronoi Scaffold was lighter in each case, that may open new opportunities in the field of additive manufacturing.
Protective Effect of Sulforaphane on Oxidative Stress and Mitochondrial Dysfunction Associated with Status Epilepticus in Immature Rats
Authors: Folbergrová Jaroslava , Ješina Pavel , Otáhal Jakub 
Published: January 2023

Abstract
The present study aimed to elucidate the effect of sulforaphane (a natural isothiocyanate) on oxidative stress and mitochondrial dysfunction during and at selected periods following status epilepticus (SE) induced in immature 12-day-old rats by Li-pilocarpine. Dihydroethidium was employed for the detection of superoxide anions, immunoblot analyses for 3-nitrotyrosine (3-NT) and 4-hydroxynonenal (4-HNE) levels and respiratory chain complex I activity for evaluation of mitochondrial function. Sulforaphane was given i.p. in two doses (5 mg/kg each), at PD 10 and PD 11, respectively. The findings of the present study indicate that both the acute phase of SE and the early period of epileptogenesis (1 week and 3 weeks following SE induction) are associated with oxidative stress (documented by the enhanced superoxide anion production and the increased levels of 3-NT and 4-HNE) and the persisting deficiency of complex I activity. Pretreatment with sulforaphane either completely prevented or significantly reduced markers of both oxidative stress and mitochondrial dysfunction. Since sulforaphane had no direct anti-seizure effect, the findings suggest that the ability of sulforaphane to activate Nrf2 is most likely responsible for the observed protective effect. Nrf2-ARE signaling pathway can be considered a promising target for novel therapies of epilepsy, particularly when new compounds, possessing inhibitory activity against protein–protein interaction between Nrf2 and its repressor protein Keap1, with less “off-target” effects and, importantly, with an optimal permeability and bioavailability properties, become available commercially.
Replies to the Critics of Knowing and Checking: an Epistemological Investigation
Authors: Melchior Guido 
Published: January 2023

AbstractThis paper replies to the comments made in Acta Analytica by Peter Baumann, Kelly Becker, Marian David, Nenad Miščević, Wes Siscoe, and Danilo Šuster on my Knowing and Checking: An Epistemological Investigation (Routledge 2019), hereinafter abbreviated as KC. These papers resulted from a workshop organized by the department of philosophy of the University of Maribor. I am very thankful to the organizers of the workshop and to the authors for their comments.
Freshwater Flow Affects Subsidies of a Copepod (Pseudodiaptomus forbesi) to Low-Salinity Food Webs in the Upper San Francisco Estuary
Authors: Hassrick Jason L. , Korman Josh , Kimmerer Wim J. , Gross Edward S. , Grimaldo Lenny F. , Lee Calvin , Schultz Andrew A. 
Published: January 2023

AbstractProductivity of the food web supporting small pelagic fishes in the upper San Francisco Estuary is chronically low, and some of the native fish species are in a long-term decline. The low-salinity (oligohaline) zone (LSZ) is particularly depauperate in phytoplankton and zooplankton. Based on prior empirical studies, it is hypothesized that freshwater flow increases the subsidy of a key copepod prey species (Pseudodiaptomus forbesi) from its freshwater population center into the LSZ. We combined hydrodynamic and particle-tracking modeling with Bayesian analysis in a box-model approach to estimate the magnitude of this subsidy and its dependence on freshwater flow rates. Net gains of P. forbesi into the LSZ came mostly from freshwater, landward regions of higher copepod abundance. The subsidy increased with freshwater flow, a finding that supports previous empirical analyses. However, in the context of persistent drought and ongoing climate change, the levels required to achieve a detectable net gain may be difficult and costly to achieve.
Tracing rail transformation: the case of passenger services in Slovenia from 1975 to 2015
Authors: Hudak Stefan Christian Alexander , Brezina Tadej , Kehrer Johannes , Schopf Josef Michael 
Published: January 2023

AbstractIn the 1990s, Slovenia was part and neighbor of a series of disruptive events, undergoing a change from member state of a diverse socialist federation to a small, independent market economy country. These comprehensive changes were followed (or accompanied by) partially dramatic changes in the transport regime. In order to trace the change of the passenger railway service a method for the analysis and characterization of the timetable offer in public transport is developed and applied to ten timetable years between 1975 and 2015 on the main lines between Ljubljana, Maribor and Zagreb. This method is based on a cluster analysis of origin–destination relations with the three variables of daily direct connections, the number of trains with synchronized intervals as well as the overall regularity of travel times. In general, an overall increase of the quality of the rail service supply is shown, focusing on the decades from 1975 to the end of the 1990s. From 2000 to 2015 relatively little changes were identified. While a very dense offer was developed in regional traffic within the wider urban areas of Ljubljana, Celje and Maribor, the direct cross-border inter-city connections to/from Zagreb have been increasingly neglected. The example of the connection between Ljubljana and Maribor demonstrates the development towards a supply-oriented integral timetable until the 1990s. Thereafter, the timetable was thinned out and gradually developed with a demand-driven principle, where a higher train density was offered to Ljubljana in the morning and to Maribor in the afternoon.
Municipal sewage sludge, aquatic weed compost on soil enzymatic activity and heavy metal accumulation in Kale (Brassica oleracea L.)
Authors: Dar Zubair Ahmad , Bhat Javeed Iqbal Ahmad , Qazi Gazala , Ganie Sartaj Ahmad , Amin Azra , Farooq Shabeena , Nazir Arifa , Rasool Akhtar 
Published: January 2023

AbstractEnormous quantities of organic wastes such as sewage sludge (SS) and aquatic weed compost (AWC) are produced in large quantities on the banks of Dal Lake Kashmir. It is a challenging task for authorities to manage them properly. Therefore, the study’s purpose was to evaluate these organic wastes agricultural use potential. The experiment was laid out in a randomized complete block design with three replications comprised of nine treatment combinations of SS, AWC and inorganic fertilizers. In the present study, the conjoint use of SS with chemical fertilizer recorded maximum build-up of soil microbial biomass carbon (MCB), urease and dehydrogenase activity with treatment T1. There were significant correlations between soil MCB and from urease and dehydrogenase activity (r2 = 0.95 and 0.97; P < 0.05), respectively. The micronutrient and heavy metal concentrations in kale exposed to SS and AWC were significantly higher than those in the untreated plants, with the highest concentration found in sole application of SS (T7). However, heavy metal concentrations were within the acceptable limits and did not overcome the maximum phytotoxic levels. The study’s finding leads to conclusion that SS along with chemical fertilizers (T1) can improve the enzymatic activity in soil, quality parameters and nutrient content in plants thereby enhancing the yield.
The Breast Cancer Single-Cell Atlas: Defining cellular heterogeneity within model cell lines and primary tumors to inform disease subtype, stemness, and treatment options
Authors: Dave Arpit , Charytonowicz Daniel , Francoeur Nancy J. , Beaumont Michael , Beaumont Kristin , Schmidt Hank , Zeleke Tizita , Silva Jose , Sebra Robert 
Published: January 2023

Abstract
Purpose
Breast Cancer (BC) is the most diagnosed cancer in women; however, through significant research, relative survival rates have significantly improved. Despite progress, there remains a gap in our understanding of BC subtypes and personalized treatments. This manuscript characterized cellular heterogeneity in BC cell lines through scRNAseq to resolve variability in subtyping, disease modeling potential, and therapeutic targeting predictions.

Methods
We generated a Breast Cancer Single-Cell Cell Line Atlas (BSCLA) to help inform future BC research. We sequenced over 36,195 cells composed of 13 cell lines spanning the spectrum of clinical BC subtypes and leveraged publicly available data comprising 39,214 cells from 26 primary tumors.

Results
Unsupervised clustering identified 49 subpopulations within the cell line dataset. We resolve ambiguity in subtype annotation comparing expression of Estrogen Receptor, Progesterone Receptor, and Human Epidermal Growth Factor Receptor 2 genes. Gene correlations with disease subtype highlighted S100A7 and MUCL1 overexpression in HER2 + cells as possible cell motility and localization drivers. We also present genes driving populational drifts to generate novel gene vectors characterizing each subpopulation. A global Cancer Stem Cell (CSC) scoring vector was used to identify stemness potential for subpopulations and model multi-potency. Finally, we overlay the BSCLA dataset with FDA-approved targets to identify to predict the efficacy of subpopulation-specific therapies.

Conclusion
The BSCLA defines the heterogeneity within BC cell lines, enhancing our overall understanding of BC cellular diversity to guide future BC research, including model cell line selection, unintended sample source effects, stemness factors between cell lines, and cell type-specific treatment response.
Alkaloids as potential antivirals. A comprehensive review
Authors: Faisal Shah , Badshah Syed Lal , Kubra Bibi , Emwas Abdul-Hamid , Jaremko Mariusz 
Published: January 2023

AbstractAlkaloids are a diverse group of natural phytochemicals. These phytochemicals in plants provide them protection against pests, and herbivorous organisms and also control their development. Numerous of these alkaloids have a variety of biological effects, and some have even been developed into medications with different medicinal properties. This review aims to provide a broad overview of the numerous naturally occurring alkaloids (isolated from both terrestrial and aquatic species) along with synthetically produced alkaloid compounds having prominent antiviral properties. Previous reviews on this subject have focused on the biological actions of both natural and synthetic alkaloids, but they have not gone into comprehensive detail about their antiviral properties. We reviewed here several antiviral alkaloids that have been described in the literature in different investigational environments i.e. (in-vivo, in-ovo, in-vitro, and in-silico), and found that these alkaloid compounds have significant antiviral properties against several infectious viruses. These alkaloids repressed and targeted various important stages of viral infection at non-toxic doses while some of the alkaloids reported here also exhibited comparable inhibitory activities to commercially used drugs. Overall, these anti-viral effects of alkaloids point to a high degree of specificity, implying that they could serve as effective and safe antiviral medicines if further pursued in medicinal and pharmacological investigations.
The Efficacy and Safety of a Human Perirenal Adipose Tissue-Derived Stromal Vascular Fraction in an Interstitial Cystitis Rat Model
Authors: Ha Ji Yong , Lee Eun Hye , Chun So Young , Lee Jun Nyung , Ha Yun-Sok , Chung Jae-Wook , Yoon Bo Hyun , Jeon Minji , Kim Hyun Tae , Kwon Tae Gyun , Yoo Eun Sang , Kim Bum Soo 
Published: January 2023

Abstract
Background:
Interstitial cystitis (IC) is a chronic and intractable disease that can severely deteriorate patients’ quality of life. Recently, stem cell therapy has been introduced as a promising alternative treatment for IC in animal models. We aimed to verify the efficacy and safety of the human perirenal adipose tissue-derived stromal vascular fraction (SVF) in an IC rat model.

Methods:
From eight-week-old female rats, an IC rat model was established by subcutaneous injection of 200 μg of uroplakin3A. The SVF was injected into the bladder submucosal layer of IC rats, and pain scale analysis, awakening cytometry, and histological and gene analyses of the bladder were performed. For the in vivo safety analysis, genomic DNA purification and histological analysis were also performed to check tumorigenicity and thrombus formation.

Results:
The mean pain scores in the SVF 20 μl group were significantly lower on days 7 and 14 than those in the control group, and bladder intercontraction intervals were significantly improved in the SVF groups in a dose-dependent manner. Regeneration of the bladder epithelium, basement membrane, and lamina propria was observed in the SVF group. In the SVF groups, however, bladder fibrosis and the expression of inflammatory markers were not significantly improved compared to those in the control group.

Conclusion:
This study demonstrated that a perirenal adipose tissue-derived SVF is a promising alternative for the management of IC in terms of improving bladder pain and overactivity.
A Proof of the Lieb–Thirring Inequality via the Besicovitch Covering Lemma
Authors: Nam Phan Thành 
Published: January 2023

AbstractWe give a proof of the Lieb–Thirring inequality on the kinetic energy of orthonormal functions by using a microlocal technique, in which the uncertainty and exclusion principles are combined through the use of the Besicovitch covering lemma.
‘A different color on an artistic palette’: teaching styles and beliefs
Authors: Çakmak İşitmez Özge , Özgür Küfi Elmaziye , Musayeva Vefalı Gülşen 
Published: January 2023

AbstractThis descriptive study attempted to explore teaching styles by eliciting beliefs of a group of English language teachers through Peacock’s (Int J Appl Linguist 11:1–20, 2001) Perceptual Teaching Style Preference Questionnaire and interviews based on Reid’s hypotheses (TESOL Q 21:87–111, 1987; Learning styles in the ESL/EFL classroom. Boston: Heinle & Heinle, 1995) on learning styles and Doyle and Rutherford’s (Theory Pract 23:20–25, 1984) suggestions on matched instructional designs. The mixed methods research design was adopted in the current study, and concurrent triangulation was used to corroborate the findings. Study findings indicated that the participants reportedly applied the group teaching style more frequently than the other styles and favored a repertoire of the visual and auditory perceptual teaching styles. Although participants were mostly positive about the importance of learning styles, effects of perceptual teaching styles on learning, and the matched instructional design, they did not believe that their students were aware of their own learning styles and noted various curricular and administrative constraints in relation to implementation of the matched instruction. Consequently, the current study suggested that the language teachers and their line managers should consider effective planning and implementation of the entire teaching operation in relation to the matched instructional design in their classrooms. Also, it is recommended that as part of their professional development, the classroom practitioners are encouraged to explore their students’ “voices” and raise their learners’ awareness regarding their learning styles for more effective teaching–learning processes.