bluuebunny/crossref_metadata_2025_split huggingface dataset:
Ketamine Effects on Energy Metabolism, Functional Connectivity and Working Memory in Healthy Humans
Authors: Driesen Naomi R. , Herman Peter , Rowland Margaret A. , Thompson Garth , Qiu Maolin , He George , Fineberg Sarah , Barron Daniel S. , Helgeson Lars , Lacadie Cheryl , Chow Robert , Gueorguieva Ralitza , Straun Teo-Carlo , Krystal John H. , Hyder Fahmeed 
Published: February 2023

AbstractWorking memory (WM) is a crucial resource for temporary memory storage and the guiding of ongoing behavior. N-methyl-D-aspartate glutamate receptors (NMDARs) are thought to support the neural underpinnings of WM. Ketamine is an NMDAR antagonist that has cognitive and behavioral effects at subanesthetic doses. To shed light on subanesthetic ketamine effects on brain function, we employed a multimodal imaging design, combining gas-free calibrated functional magnetic resonance imaging (fMRI) measurement of oxidative metabolism (CMRO2), resting-state cortical functional connectivity assessed with fMRI, and WM-related fMRI. Healthy subjects participated in two scan sessions in a randomized, double-blind, placebo-controlled design. Ketamine increased CMRO2and cerebral blood flow (CBF) in prefrontal cortex (PFC) and other cortical regions. However, resting-state cortical functional connectivity was not affected. Ketamine did not alter CBF-CMRO2coupling brain-wide. Higher levels of basal CMRO2were associated with lower task-related PFC activation and WM accuracy impairment under both saline and ketamine conditions. These observations suggest that CMRO2and resting-state functional connectivity index distinct dimensions of neural activity. Ketamine’s impairment of WM-related neural activity and performance appears to be related to its ability to produce cortical metabolic activation. This work illustrates the utility of direct measurement of CMRO2via calibrated fMRI in studies of drugs that potentially affect neurovascular and neurometabolic coupling.
Optimizing linear ion trap data independent acquisition towards single cell proteomics
Authors: Phlairaharn Teeradon , Ye Zilu , Krismer Elena , Pedersen Anna-Kathrine , Pietzner Maik , Olsen Jesper V. , Schoof Erwin M. , Searle Brian C. 
Published: February 2023

ABSTRACTA linear ion trap (LIT) is an affordable, robust mass spectrometer that proves fast scanning speed and high sensitivity, where its primary disadvantage is inferior mass accuracy compared to more commonly used time-of-flight (TOF) or orbitrap (OT) mass analyzers. Previous efforts to utilize the LIT for low-input proteomics analysis still rely on either built-in OTs for collecting precursor data or OT-based library generation. Here, we demonstrate the potential versatility of the LIT for low-input proteomics as a stand-alone mass analyzer for all mass spectrometry measurements, including library generation. To test this approach, we first optimized LIT data acquisition methods and performed library-free searches with and without entrapment peptides to evaluate both the detection and quantification accuracy. We then generated matrix-matched calibration curves to estimate the lower limit of quantification using only 10 ng of starting material. While LIT-MS1 measurements provided poor quantitative accuracy, LIT-MS2 measurements were quantitatively accurate down to 0.5 ng on column. Finally, we optimized a suitable strategy for spectral library generation from low-input material, which we used to analyze single-cell samples by LIT-DIA using LIT-based libraries generated from as few as 40 cells.
Testing the efficacy of different molecular tools for parasite conservation genetics: a case study using horsehair worms (Phylum Nematomorpha)
Authors: De Vivo Mattia , Chen Wei-Yun , Huang Jen-Pan 
Published: February 2023

AbstractIn recent years, parasite conservation has become a globally significant issue. Because of this, there is a need for standardised methods for inferring population status and possible cryptic diversity. However, given the lack of molecular data for some groups, it is challenging to establish procedures for genetic diversity estimation. Therefore, universal tools, such as double digest restriction-site associated DNA sequencing (ddRADseq), could be useful when conducting conservation genetic studies on rarely studied parasites. Here, we generated a ddRADseq dataset that includes all three described Taiwanese horsehair worms (Phylum Nematomorpha), possibly one of the most understudied animal groups. Additionally, we produced data for a fragment of the cytochrome c oxidase subunit I (COXI) for said species. We used the COXI dataset in combination with previously published sequences of the same locus for inferring the effective population size (Ne) trends and possible population structure.We found that a larger and geographically broader sample size combined with more sequenced loci resulted in a better estimation of changes inNe. We were able to detect demographic changes associated with Pleistocene events in all the species. Furthermore, the ddRADseq dataset forChordodes formosanusdid not reveal a genetic structure based on geography, implying a great dispersal ability, possibly due to its hosts. We showed that different molecular tools can be used to reveal genetic structure and demographic history at different historical times and geographical scales, which can help with conservation genetic studies in rarely studied parasites.
In VitroAntibacterial Activity of Dinuclear Thiolato-Bridged Ruthenium(II)-Arene Compounds
Authors: Bugnon Quentin , Melendez Camilo , Desiatkina Oksana , de Chaptes Louis Fayolles Chorus , Holzer Isabelle , Păunescu Emilia , Hilty Markus , Furrer Julien 
Published: February 2023

AbstractThe antibacterial activity of 22 thiolato-bridged dinuclear ruthenium(II)-arene compounds was assessedin vitroagainstEscherichia coli, Streptococcus pneumoniaeandStaphylococcus aureus. None of the compounds efficiently inhibited the growth of the threeE. colistrains tested and only compound5exhibited a medium activity against this bacterium (MIC (minimum inhibitory concentration) of 25 μM). However, a significant antibacterial activity was observed againstS. pneumoniae, with MIC values ranging from 1.3 to 2.6 μM for compounds1-3,5and6. Similarly, compounds2,5-7and20-22had MIC values ranging from 2.5 to 5 μM againstS. aureus.The tested diruthenium compounds have a bactericidal effect significantly faster than that of penicillin. Fluorescence microscopy assays performed onS. aureususing the BODIPY-tagged diruthenium complex15showed that this type of metal compound enter the bacteria and do not accumulate in the cell wall of gram-positive bacteria. Cellular internalization was further confirmed by inductively coupled plasma mass spectrometry (ICP-MS) experiments. The nature of the substituents anchored on the bridging thiols and the compounds molecular weight appear to significantly influence the antibacterial activity. Thus, if overall a decrease of the bactericidal effect with the increase of compounds’ molecular weight is observed, however the complexes bearing larger benzo-fused lactam substituents had low MIC values. This first antibacterial activity screening demonstrated that the thiolato-diruthenium compounds exhibit promising activity againstS. aureusandS. pneumoniaeand deserve to be considered for further studies.
Reliability of sucrose preference testing following short or no food and water deprivation - a Systematic Review and Meta–Analysis of rat models of chronic unpredictable stress
Authors: Berrio Jenny P. , Hestehave Sara , Kalliokoski Otto 
Published: February 2023

AbstractThe sucrose preference test is a popular test for anhedonia in the chronic unpredictable stress model of depression. Yet, the test does not always produce consistent results. Long food and water deprivation before the test, while often implemented, confounds the results by introducing unwanted drives in the form of hunger and thirst. We assessed the reliability of the test when only short or no fasting was used. We searched PubMed, Embase, and Web of Science for studies in rats exposed to chronic unpredictable stress that used no more than six hours of food and/or water deprivation before the test. Sweet consumptions, for stressed and control/antidepressant-treated animals, in 132 studies were pooled using random effects models. We found a decrease in sweet consumption in stressed rats, compared to controls, that was halved when a non-caloric sweetener was used and significantly reduced when sucrose consumption was corrected for body weight. What is more, the length of food and water deprivation was found to confound the effect. The effect was reversed when the stressed rats were treated with antidepressants. Methodological strategies meant to control for recognized sources of bias when conducting the test were often missing, and so was a clear and complete report of essential study information. Our results indicate that not only is food and water deprivation before the test unnecessary, but not recommended. Even in absence of long fasting, we found evidence of an additional effect on sweet consumption that is unrelated to anhedonia. Without properly controlling for non-hedonic drivers of consumption, the test is unreliable as a proxy measure of anhedonia. Strengthening the methodological rigor and addressing the confounding effect of metabolic factors in the sucrose preference test prevents misleading conclusions that harm the translatability of the associated research and perpetuates the use of animals for little gain.
The gut lactic acid bacteria metabolite, 10-oxo-cis-6,trans-11-octadecadienoic acid, suppresses inflammatory bowel disease in mice by modulating the NRF2 pathway and GPCR-signaling
Authors: Ando Miki , Nagata Kazuki , Ito Naoto , Noguchi Sakura , Yamamoto Asuka , Kodama Naoki , Yashiro Takuya , Hachisu Masakazu , Ichihara Gaku , Kishino Shigenobu , Yamamoto Masayuki , Ogawa Jun , Nishiyama Chiharu 
Published: February 2023

AbstractVarious gut bacteria, includingLactobacillus plantarum, possess several enzymes that produce hydroxy fatty acids (FAs), oxo FAs, conjugated FAs, and partially saturated FAs from polyunsaturated FAs as secondary metabolites. Among these derivatives, we identified 10-oxo-cis-6,trans-11-octadecadienoic acid (γKetoC), a γ-linolenic acid-derived enon FA, as the most effective immunomodulator, which inhibited the antigen-induced immunoactivation and the LPS-induced production of inflammatory cytokines. The treatment with γKetoC markedly increased the protein level of NRF2, a master transcription factor for antioxidant responses, and the mRNA level ofHmox1, a target gene of NRF2, in bone marrow-derived dendritic cells (BMDCs). Although γKetoC significantly suppressed the LPS-induced activation of control BMDCs, particularly the secretion of IL-12/23p40, the suppressive effects of γKetoC were reduced inNrf2-/-BMDCs. GW9508, an agonist of GPR40/GPR120, inhibited the release of cytokines from LPS-stimulated BMDCs without activating the NRF2 pathway. We evaluated the role of NRF2 in the anti-inflammatory effects of γKetoC in a dextran sodium sulfate-induced colitis model. The oral administration of γKetoC significantly reduced body weight loss, improved stool scores, and attenuated atrophy of the colon, in wild-type C57BL/6J andNrf2+/-(C57BL/6N) mice with colitis. In contrast, the pathology of colitis was deteriorated inNrf2-/-mice even with the administration of γKetoC.Collectively, the present results demonstrated the involvement of the NRF2 pathway in γKetoC-mediated anti-inflammatory responses.
More warm‐adapted species in soil seed banks than in herb layer plant communities across Europe
Authors: Auffret Alistair G. , Vangansbeke Pieter , De Frenne Pieter , Auestad Inger , Basto Sofía , Grandin Ulf , Jacquemyn Hans , Jakobsson Anna , Kalamees Rein , Koch Marcus A. , Marrs Rob , Marteinsdóttir Bryndís , Wagner Markus , Bekker Renée M. , Bruun Hans Henrik , Decocq Guillaume , Hermy Martin , Jankowska‐Błaszczuk Małgorzata , Milberg Per , Måren Inger E. , Pakeman Robin J. , Phoenix Gareth K. , Thompson Ken , Van Calster Hans , Vandvik Vigdis , Plue Jan 
Published: February 2023

Abstract

Responses to climate change have often been found to lag behind the rate of warming that has occurred. In addition to dispersal limitation potentially restricting spread at leading range margins, the persistence of species in new and unsuitable conditions is thought to be responsible for apparent time‐lags.

Soil seed banks can allow plant communities to temporarily buffer unsuitable environmental conditions, but their potential to slow responses to long‐term climate change is largely unknown. As local forest cover can also buffer the effects of a warming climate, it is important to understand how seed banks might interact with land cover to mediate community responses to climate change.

We first related species‐level seed bank persistence and distribution‐derived climatic niches for 840 plant species. We then used a database of plant community data from grasslands, forests and intermediate successional habitats from across Europe to investigate relationships between seed banks and their corresponding herb layers in 2763 plots in the context of climate and land cover.

We found that species from warmer climates and with broader distributions are more likely to have a higher seed bank persistence, resulting in seed banks that are composed of species with warmer and broader climatic distributions than their corresponding herb layers. This was consistent across our climatic extent, with larger differences (seed banks from even warmer climates relative to vegetation) found in grasslands.

Synthesis. Seed banks have been shown to buffer plant communities through periods of environmental variability, and in a period of climate change might be expected to contain species reflecting past, cooler conditions. Here, we show that persistent seed banks often contain species with relatively warm climatic niches and those with wide climatic ranges. Although these patterns may not be primarily driven by species' climatic adaptations, the prominence of such species in seed banks might still facilitate climate‐driven community shifts. Additionally, seed banks may be related to ongoing trends regarding the spread of widespread generalist species into natural habitats, while cool‐associated species may be at risk from both short‐ and long‐term climatic variability and change.
Women's preconception health in England: a report card based on cross‐sectional analysis of national maternity services data from 2018/2019
Authors: Schoenaker Danielle A. J. M. , Stephenson Judith , Smith Helen , Thurland Kate , Duncan Helen , Godfrey Keith M. , Barker Mary , Singh Claire , Alwan Nisreen A. ,   for the UK Preconception Partnership
Published: February 2023

AbstractObjectiveTo present the first national‐level report card on the state of women's preconception health in England.DesignCross‐sectional population‐based study.SettingMaternity services, England.PopulationAll pregnant women in England with a first antenatal (booking) appointment recorded in the national Maternity Services Dataset (MSDS) from April 2018 to March 2019 (n = 652 880).MethodsWe analysed the prevalence of 32 preconception indicator measures in the overall population and across socio‐demographic subgroups. Ten of these indicators were prioritised for ongoing surveillance based on modifiability, prevalence, data quality and ranking by multidisciplinary UK experts.ResultsThe three most prevalent indicators were the proportion of the 22.9% of women who smoked 1 year before pregnancy who did not quit smoking before pregnancy (85.0%), those who had not taken folic acid supplementation before pregnancy (72.7%) and previous pregnancy loss (38.9%). Inequalities were observed by age, ethnicity and area‐based deprivation level. The ten indicators prioritised were not taking folic acid supplementation before pregnancy, obesity, complex social factors, living in the most deprived areas, smoking around the time of conception, overweight, pre‐existing mental health condition, pre‐existing physical health condition, previous pregnancy loss and previous obstetric complication.ConclusionsOur findings suggest important opportunities to improve the state of preconception health and reduce socio‐demographic inequalities for women in England. In addition to MSDS data, other national data sources that record further and possibly better quality indicators could be explored and linked to build a comprehensive surveillance infrastructure.
Assessment of subclinical left ventricular systolic dysfunction in patients with type 2 diabetes: Relationship with HbA1c and microvascular complications
Authors: Chen Yanyan , Zhang Ying , Wang Yi , Ta Shengjun , Shi Min , Zhou Yingni , Li Mengying , Fu Jianfang , Wang Li , Liu Xiangyang , Lu Zuowei , Liu Liwen , Li Zeping , Zhou Jie , Li Xiaomiao 
Published: February 2023

AbstractBackgroundWe aimed to examine the association between glycated hemoglobin (HbA1c), microvascular complications, and subclinical left ventricular (LV) systolic dysfunction, and to determine the strength of the correlation in asymptomatic patients with type 2 diabetes mellitus (T2DM).MethodsGlobal longitudinal strain (GLS) was employed to assess the subclinical LV function of 152 enrolled T2DM patients with preserved LV ejection fraction, with the cutoff for subclinical LV systolic dysfunction predefined as GLS < 18%.ResultsAccording to univariate analysis, the reduced GLS exhibited association with the clinical features including HbA1c, triglyceride, systolic blood pressure, fasting glucose, heart rate, diabetic retinopathy, and urinary albumin creatinine ratio (UACR) (all p < .05). After the factors of gender, age, and related clinical covariables adjusted, multiple logistic regression analysis revealed the HbA1c (odds ratio [OR] 1.66; 95% confidence interval [CI] 1.30–2.13; p < .001), UACR (OR 2.48; 95% CI 1.12–5.47; p = .025) and triglyceride (OR 1.84; 95% CI 1.12–3.03; p = .017) as the independent risk factors for the reduced GLS. Receiver operating characteristic curve showed a predictive value of the HbA1c for the subclinical LV systolic dysfunction (area under curve: 0.74; p < .001).ConclusionsIn asymptomatic T2DM patients, subclinical LV systolic dysfunction was associated with HbA1c, diabetic complications, and triglyceride. More prominently, HbA1c may exert a prognostic significance for the progression of myocardial damage.
The use of different 16S rRNA gene variable regions in biogeographical studies
Authors: Varliero Gilda , Lebre Pedro H. , Stevens Mark I. , Czechowski Paul , Makhalanyane Thulani , Cowan Don A. 
Published: February 2023

Abstract16S rRNA gene amplicon sequencing is routinely used in environmental surveys to identify microbial diversity and composition of the samples of interest. The dominant sequencing technology of the past decade (Illumina) is based on the sequencing of 16S rRNA hypervariable regions. Online sequence data repositories, which represent an invaluable resource for investigating microbial distributional patterns across spatial, environmental or temporal scales, contain amplicon datasets from diverse 16S rRNA gene variable regions. However, the utility of these sequence datasets is potentially reduced by the use of different 16S rRNA gene amplified regions. By comparing 10 Antarctic soil samples sequenced for five different 16S rRNA amplicons, we explore whether sequence data derived from diverse 16S rRNA variable regions can be validly used as a resource for biogeographical studies. Patterns of shared and unique taxa differed among samples as a result of variable taxonomic resolutions of the assessed 16S rRNA variable regions. However, our analyses also suggest that the use of multi‐primer datasets for biogeographical studies of the domain Bacteria is a valid approach to explore bacterial biogeographical patterns due to the preservation of bacterial taxonomic and diversity patterns across different variable region datasets. We deem composite datasets useful for biogeographical studies.
Sleep disturbances in Norwegian children with fetal alcohol spectrum disorders (FASD) with and without a diagnosis of attention‐deficit hyperactivity disorder or epilepsy
Authors: Gerstner Thorsten , Sævareid Hans Inge , Johnsen Åse Ribe , Løhaugen Gro , Skranes Jon 
Published: February 2023

AbstractBackgroundFetal alcohol spectrum disorder (FASD) describes a combination of developmental, cognitive, and behavioral disabilities in children with prenatal exposure to alcohol. The literature suggests that there are higher rates of sleep disturbances in these children. Few studies have investigated sleep disturbances in relation to common comorbidities of FASD. We examined the prevalence of disturbed sleep and the relationship between parent‐reported sleep problems in different FASD subgroups and comorbidities like epilepsy or attention‐deficit hyperactivity disorder (ADHD) and impact on clinical functioning.MethodsIn this prospective cross‐sectional survey, caregivers of 53 children with FASD completed the Sleep Disturbance Scale for Children (SDSC). Information about comorbidities was collected, and EEG and assessment of IQ, daily‐life executive and adaptive functioning were performed. Group comparisons and ANCOVA interaction models were used to test the associations between different sleep disturbances and clinical factors that could interfere with sleep.ResultsAn abnormal sleep score on the SDSC was very common, affecting 79% of children (n = 42) with equal prevalence in all FASD subgroups. Difficulty falling asleep was the most common sleep problem, followed by difficulty staying asleep and waking early. The incidence of epilepsy was 9.4%, with an abnormal EEG seen in 24.5%, and a diagnosis of ADHD in 47.2% of children. The distribution of these conditions was equal in all FASD subgroups. Children with signs of sleep disturbance had poorer working memory, executive function, and adaptive functioning. Children with ADHD had a greater prevalence of sleep disturbance than those without ADHD (OR 1.36; 95% CI 1.03 to 1.79).ConclusionProblems with sleep are very common in FASD children and seem independent of FASD subgroup and the presence of epilepsy or a pathological EEG finding, while those with ADHD had more sleep problems. The study underscores the importance of screening for sleep disturbances in all children with FASD as these problems may be treatable.