bluuebunny/crossref_metadata_2025_split huggingface dataset:
ProvitaminA, lysine and tryptophan enrichment in shrunken2-based sweet corn genotypes through genomics-assisted breeding for crtRB1 and opaque2 genes
Authors: Singh Bhavna , Zunjare Rajkumar U. , Srivast Smriti , Chand Gulab , Gain Nisrita , Bhatt Vinay , Muthusamy Vignesh , Hossain Firoz 
Published: February 2023

Abstract
Background Malnutrition affects large section of population worldwide. Vitamin-A and protein deficiencies have emerged as the major global health-issue. Traditional shrunken2 (sh2)-based sweet corn is deficient in provitaminA (proA), lysine and tryptophan. Natural variant of β-carotene hydroxylase1 (crtRB1) and opaque2 (o2) enhances proA, lysine and tryptophan in maize. So far, no sweet corn hybrid rich in these nutrients has been released elsewhere. Development of biofortified sweet corn hybrids would help in providing the balanced nutrition.
Methods and Results We targeted three sh2-based sweet corn inbreds (SWT-19, SWT-20 and SWT-21) for introgression of mutant crtRB1 and o2 genes using molecular breeding. The gene-based 3′TE-InDel and SSR (umc1066) markers specific to crtRB1 and o2, respectively were utilized in foreground selection in BC1F1, BC2F1 and BC2F2. Segregation distortion was observed for crtRB1 and o2 genes in majority of populations. Background selection using 91-100 SSRs revealed recovery of recurrent parent genome (RPG) up to 96%. The introgressed progenies possessed significantly higher proA (13.56 µg/g), lysine (0.336%) and tryptophan (0.082%) over original versions (proA: 2.70 µg/g; lysine: 0.154% and tryptophan: 0.038%). Kernel sweetness among introgressed progenies (17.3%) was comparable to original sweet corn (17.4%). The introgressed inbreds exhibited higher resemblance with their recurrent parents for yield and morphological characters.
Conclusion These newly developed biofortified sweet corn genotypes hold immense promise to alleviate malnutrition.
Exploration on Ability of Printable modified papers for the application in heat sublimation transfer printing of polyester fabric
Authors: Adel Abeer , Ahmed Nivin , Diab Mohamed , El-Shall Fatma , El-Shinnawy Nabila 
Published: February 2023

Abstract
Heat transfer papers in this work were loaded with a new core-shell pigment based on precipitating thin shell of titanium dioxide on a core of rice husk silica ash TiO2/RHSA to be applied in dye sublimation printing of textile fabrics. Besides, 0.1%(w/w) cationic polyacrylamide (CPAM) and 1%(w/w) bentonite (Bt) were also added sequentially to improve drainage and filler retention of the paper hand-sheets made from bleached kraft bagasse pulps. The effect of the new core-shell pigment on the mechanical and barrier properties, thermal stability and surface morphology of modified paper sheets were investigated. In addition, the study of transfer printability and ease of dye release from paper to fabric in this heat transfer printing of polyester fabrics using silkscreen printing under different transfer parameters were studied. In addition, fastness measurements including washing, light and perspiration of printing polyester fabric were also estimated.
Blow up solutions for 2-dimensional semilinear elliptic of liouville type with nonlinear gradient term
Authors: Baraket Sami , Ghorbal Anis Ben , Mtiri Foued 
Published: February 2023

Abstract
The existence of singular limit solutions are investigated by establishing a new Liouville type theorem for nonlinear elliptic system by using the nonlinear domain decomposition method.
Removal of calciprotein particles from the blood using an adsorption column improves prognosis of hemodialysis miniature pigs
Authors: Miura Marina , Miura Yutaka , Iwazu Yoshitaka , Mukai Hideyuki , Sugiura Takahiro , Suzuki Yuji , Kato Masami , Kano Mayumi , Nagata Daisuke , Shiizaki Kazuhiro , Kurosu Hiroshi , Kuro-o Makoto 
Published: February 2023

Abstract
Hyperphosphatemia is a major risk for poor prognosis in patients with end-stage renal disease1. However, the molecular mechanism behind this link remains elusive. We and others have demonstrated that serum phosphorus levels correlate positively with circulating levels of calciprotein particles (CPPs)2,3. CPPs are colloidal mineral-protein complexes containing insoluble calcium-phosphate precipitates and have been reported to induce calcification in cultured vascular smooth muscle cells and inflammatory responses in cultured macrophages4,5. Hence, we hypothesize that CPPs may be responsible for disorders associated with hyperphosphatemia. Using hyperphosphatemic miniature pigs receiving hemodialysis, here we show that removal of CPPs from the blood with a newly developed CPP adsorption column improves survival and alleviates complications including coronary artery calcification, vascular endothelial dysfunction, metastatic pulmonary calcification, left ventricular hypertrophy, and chronic inflammation. The present study identifies CPPs as an effective therapeutic target and justifies clinical trials to determine whether the CPP adsorption column may be useful as a medical device for improving clinical outcomes of hemodialysis patients.
Analysis and Experimental Study on Resistance-increasing Behavior of Compound High Efficiency Autonomous Inflow Control Device
Authors: Dong LL. , Zhang YL. 
Published: February 2023

Abstract
Bottom water coning is the main reason to reduce the recovery of horizontal bottom water reservoir. By water coning, we mean the oil-water interface changes from a horizontal state to a mound-shaped cone and breaks through to the wellbore. Autonomous inflow control device (AICD) is an important instrument maintain normal production after bottom water coning, however, the water restricted effect is seriously affected by its insufficient resistance-increasing ability to water. Aiming this problem, this paper designs a multi-stage resistance-increasing and composite AICD. The separation mechanism of oil-water two phases in this structure, the resistance form of oil-water single phase and the resistance-increasing principle of water phase are analyzed. Establish the dual-phase multi-stage separation and resistance-increasing model, and verified by measuring the throttling pressure drop and oil-water volume fraction of the AICD. Structural parameters of the AICD and different fluid properties in how to affect the throttle pressure and oil -water volume fraction are systematically investigated in this paper. The results show that the higher the water content of fluid is, the better the water-resistance effect of the device is, at the same time, the device has good adaptability to oil viscosity and density. After optimization analysis, better structural parameters of AICD are obtained. The simulation calculation results show that, compared with the existing spiral-flow type AICD, water control performance of the composite AICD is improved, the water throttling pressure drop is increased by 4.5 times on average, and the oil-water volume fraction ratio is increased by 5.3 times on average. This study provides a method for improving recovery efficiency of bottom water reservoir and a water control device.
Ball end micro milling of brass: Wear behavior in field-use
Authors: Klauer-Dobrowolski Katja , Eifler Matthias , Kirsch Benjamin , Seewig Jörg , Aurich Jan C. 
Published: February 2023

Abstract
Micro milling is a precise method for micro structuring component surfaces. When complex or free-form structures are to be manufactured, ball end milling tools are particularly suitable. In preliminary studies by the authors, areal material measures for the calibration of optical topography measuring instruments were manufactured via micro milling. The demands on the surface quality of the material measures are extremely high. Therefore, worn tools must not be used if the wear has a direct effect on the resulting topography of the manufactured material measures. At the same time, the micro milling tools used are very costly and should therefore be used as long as possible. A comprehensive experimental study was carried out on the wear behavior of different ball end micro milling tools. In the investigations, brass CuZn39Pb3 was machined and different areal material measures were manufactured. The tool life dependent wear was determined and critical wear was identified.
Behavioural contagion in wild spider monkeys (Ateles geoffroyi)
Authors: Valdivieso-Cortadella Sara , Bernardi-Gómez Chiara , Aureli Filippo , Llorente Miquel , Amici Federica 
Published: February 2023

Abstract
Behavioural contagion is a widespread phenomenon in animal species, which is thought to promote coordination and group cohesion. Among non-human primates, however, evidence of behavioural contagion is currently limited to few Catarrhine species. Here, we investigated whether behavioural contagion is also present in Platyrrhines (i.e. primates from South and Central America), by assessing yawning and scratching contagion in a wild group (N = 49) of Geoffroy’s spider monkeys (Ateles geoffroyi). We conducted focal samples to examine whether individuals observing the triggering event (i.e. a naturally occurring yawning or scratching event in the group) would be more likely to yawn or scratch in the following three minutes, as compared to individuals who did not observe the triggering event. We ran generalized linear mixed models using a Bayesian approach, and found that the probability of yawning and scratching was higher for individuals observing others yawning and scratching, respectively, as compared to individuals who did not observe such an event. However, behavioural contagion did not vary depending on the observer’s sex, kinship or relationship quality with the individual performing the triggering event. These findings provide the first evidence for yawning and scratching contagion in a wild group of spider monkeys, and importantly contribute to the debate about the evolutionary origins of behavioural contagion in primates, which is likely to have emerged before the evolutionary split between Platyrrhines and Catarrhines.
Supraspinal facilitation of nociception by glutamatergic innervation from the retrosplenial cortex to anterior cingulate cortex
Authors: Xue Man , Hao Shun , Chen Qi-Yu , Shi Wan-Tong , Li Xu-hui , Lu Jing-Shan , Xu Fang , Bi Guo-Qiang , Zhuo Min 
Published: February 2023

Abstract

Anterior cingulate cortex (ACC) is critical for pain perception; while the retrosplenial cortex (RSC) plays an important role in navigation and memory. It is known that RSC projects to ACC, less is known about the function of this projection. Here we used integrative approaches to show that there is direct excitatory glutamatergic projections from the RSC to the ACC, and postsynaptic excitatory responses are mediated by AMPA receptors. Activation of RSC-ACC by optogenetics significantly facilitated behavioral responses to nociceptive stimuli (mechanical and thermal) in freely moving mice. By contrast, spinal nociceptive tail-flick and anxiety-like activities were not affected. Our results suggest that RSC to ACC projection preferentially affects nociceptive process in supraspinal cortical region. Our results have identified a new facilitatory cortico-cortical circuit for sensory nociceptive process, and this pathway serves to link memory ‘engram’ to pain perception in humans and animals.
Hcy regulates SIRT1-STAT3 phosphorylation by upregulating WWP2 expression and induce VSMCs proliferation, migration, and phenotypic switch.
Authors: Wang Xiuyu , Ma Xing , Zeng Yue , Pokharel Smritee , zhang minghao 
Published: February 2023

Abstract

The proliferation, migration, and cellular morphology of vascular smooth muscle cells (VSMCs) play important roles in the pathogenesis of atherosclerosis (AS). Homocysteine (Hcy) is a sulfur-containing amino acid, which is an intermediate product of methionine metabolism. Hcy can induce proliferation, migration, and phenotypic switch of VSMCs, but details of these mechanisms are still unclear. NAD-dependent deacetylase sirtuin-1 (SIRT1-STAT3) signaling pathway is involved in various cellular functions. Here, we sought to determine if this multifunctional pathway played a role in Hcy-induced proliferation, migration, and phenotypic transformation of VSMCs, which has not been previously reported. NEDD4-like E3 ubiquitin-protein ligase WWP2 (WWP2) is involved in VSMCs phenotypic modulation and can be a potential target in the treatment of various cardiovascular diseases. In our study, Serum Hcy of the ApoE-/-mice fed 2% high-methionine diet was significantly higher than that of the normal diet group (P < 0.01), and the serum Hcy concentrations exceeded 20 µmol/L, indicating that the HHcy animal model was successfully replicated. ApoE-/-+HMD mouse showed changes in the expression level of aortic proteins, α-SMA and SM22α were decreased (P < 0.05) in aortic VSMCs, while osteopontin (OPN) increased (P < 0.05). Similarly, WWP2 mRNA and protein expression were increased (P < 0.01) in the aortic vasculature of ApoE-/-+HMD. In vitro, WWP2 mRNA and protein expressions were higher in the Hcy group (P < 0.01), and the results were consistent with the animal model. Overexpression of WWP2 promoted Hcy-induced VSMCs proliferation, migration, and transformation from contractile to synthetic phenotype, while interference with WWP2 had the opposite effect. The protein expression of SIRT1 in the Hcy group was decreased (P < 0.01) and p-STAT3 increased (P < 0.05). Overexpression of WWP2 significantly decreased (P < 0.05) SIRT1 and increased (P < 0.05) p-STAT3, while the inhibition of WWP2 had the opposite effect. Inhibition of SIRT1 increased (P < 0.01) p-STAT3 in contrast to the inhibition (P < 0.01) of p-STAT3 following SIRT1 stimulation in the Hcy group. Taken together, the results of the present study indicate that WWP2 promotes Hcy-induced VSMC proliferation, migration, and phenotype switch by regulating SIRT1-STAT3 phosphorylation.
Hospital and urban wastewaters shape the structure and active resistome of environmental biofilms
Authors: Buelow Elena , Dauga Catherine , Carrion Claire , Mathé-Hubert Hugo , Achaibou Sophia , Gaschet Margaux , Jové Thomas , Chesneau Olivier , Kennedy Sean P. , Ploy Marie-Cecile , Re Sandra Da , Christophe Dagot 
Published: February 2023

Abstract
Background
Demonstration of the transfer, dynamics, and regulation of antibiotic resistance genes (ARGs) and mobile genetic elements (MGEs) in a complex environmental matrix is yet experimentally challenging, with many essential open questions such as how and where transfer and dissemination of ARGs happens in nature. The extent and conditions of MGEs transfer that carry ARGs is still largely unexplored in natural environments and microbial communities. Biofilms are structures that include high density multi-species bacterial communities embedded in self-produced extracellular polymeric substances (EPS) constituting a matrix that facilitates gene transfer and where bacteria exhibit high tolerance to stress and to antibiotics. In this study we implemented a sampling and analysis approach that allows phenotypic and genomic analyses of in situ and reconstituted in vitro hospital and urban wastewater (WW) biofilms. To assess the potential of hospital and urban WW biofilms to efficiently disseminate ARGs in the WW system, we explored the EPS within the biofilm matrix and assessed the expression of the resistome (ARGs) and mobilome (MGEs) by metatranscriptomics.
Results
We first showed that a) the composition of EPS differs depending on their growth environment (in situ and in vitro) and their sampling origin (hospital vs urban WW) and that b) a low amount of ciprofloxacin impacted the composition of the EPS. Next, the metatranscriptomic approach showed that a) expression of ARGs and MGEs increase upon adding a low amount of ciprofloxacin for biofilms from hospital WW but not for those from urban WW and b) that expression of specific plasmids that carry individual or multiple ARGs varies depending on the WW origins of the biofilms. When the same plasmids were expressed in both, urban and hospital WW biofilms, they carried and expressed different ARGs.
Conclusion
We show that hospital and urban wastewaters shape the structure and active resistome of environmental biofilms, and we confirmed that hospital WW is an important hot spot for the dissemination and selection of AMR. The different responses to antibiotic pressure in hospital vs urban biofilms, coupled with differences in biofilm structure helps delineate distinct characteristics of hospital and urban WW biofilms highlighting the relationships between the resistome and its expression in environmental biofilms and their surrounding ecosystems.
The effect of healthy lifestyle behaviors on orthorexia nervosa in pregnant women
Authors: Ke Müjde , Kaplan Mehmet 
Published: February 2023

Abstract
Purpose
In order to protect maternal and fetal health during pregnancy, nutrient intake must be adequate and balanced. Inadequate and unbalanced nutrition during pregnancy negatively affects maternal and fetal health and causes maternal and infant mortality as well as low birthweight and prematurity. Therefore, the aim of this study was to determine whether the sociodemographic characteristics and healthy lifestyle behaviors of pregnant women have an effect on the level of Orthorexia Nervosa.
Method
The study was planned in a descriptive-relational design. The study sample consisted of 234 pregnant women aged 18 years and over who applied to a state hospital in a provincial center located in Eastern Turkey. The data were collected using the socio-demographic information form, the Orthorexia Nervosa Scale, and the Healthy Lifestyle Behavior Scale.
Results
The majority of the pregnant women participating in the study were in the 3rd trimester and non-smokers, 56.6% had a BMI within the normal ranges and the prevalence of Orthorexia Nervosa was found to be 45%. It was determined that there was a significant positive correlation between BMI and orthorexia nervosa and a significant negative correlation between healthy lifestyle behaviors and orthorexia nervosa.
Conclusion
Healthy lifestyle behaviors were found to affect the occurrence of orthorexia nervosa at a rate of 22.4%. In addition, it was determined that BMI, age, smoking, education level, and working status were effective in exhibiting orthorexic behaviors of pregnant women.
Level of Evidence:
Level V, cross-sectional descriptive study
A safe production process of alkylene oxides from alkylene carbonates
Authors: Polotti Gianmarco , Barbieri Massimo , Moscatelli Davide 
Published: February 2023

Abstract
Alkylene oxides of low molecular weight, ethylene, and propylene oxides, have a widespread use in industry. They are used to produce other chemicals and products such as solvents, surfactants, antifreeze, adhesives, polyurethane foams, and pharmaceuticals. They are also used as fumigants in agricultural products, and as a sterilant for medical equipment and supplies. Unfortunately, it is worth noting that alkylene oxides also possess several physical and health hazards: They are flammable, explosive, and their storage and transportation warrant stringent safety rules. The obvious solution to reduce the risk is to generate them from intermediate safety materials and only when and where they are necessary. The literature suggests that correspondent carbonates are very stable precursors. The scope of our study is that of identifying the optimal conditions to generate ethylene and propylene oxide in the moment just before their reaction and eventual use. The patent deals with some equipment and process conditions that guarantee the fast and reliable dissociation of carbonates in their correspondent oxides. The natural output of the patent is a unit able to produce the dangerous material in situ, under controlled conditions. As a result, all the risks related to transportation and storage are eliminated.
A Method for Evaluation of e-Government systems information Agility: Case of e-Algeria
Authors: Soumia Aggoune , Amine Riahla Mohamed 
Published: February 2023

Abstract
Development of e-government evaluation frameworks began around 2000s. Most of the developed approaches are technologically driven; they focus on “e” rather than “government”. Moreover, they tend to evaluate tangible measures (such as costs, benefits, etc.) and neglects important intangible measures (such as agility, sustainability, etc.). Evaluation of agility within e-government has proved to be important but complex. The importance is due to the increasing need for governments to justify investments, assess impacts and monitor progress in the ever-changing environment. The complexity is due to multi-disciplinary of the concept, the difficulty inherent in quantifying its –intangible- dimensions and developing appropriate evaluative parameters and metrics. This paper aims make a contribution to the journal by addressing all these considerations through a practical method for agility evaluation. This method is applied within a real case study, e-Algeria project; as part of the review of 10 years since its launch in 2013. The objective was to evaluate the agility of TAWASSOL framework that is designed to be one-stop-shop for government services.
Odd presentation of shunt malfunction: a case series and review of literature
Authors: Kankam Samuel Berchi , Karami Sajedeh , Nejat Amirhosein , Meybodi Keivan Tayyebi , Habibi Zohreh , Nejat Farideh 
Published: February 2023

Abstract
Objective
Children with previous ventriculoperitoneal shunt (VPS) insertion may refer to the hospital with various clinical complaints. Shunt malfunction is frequently diagnosed in these children necessitating shunt revision. Although increased head circumference, setting sun eye in younger children and headache, nausea/ vomiting, loss of consciousness, visual disturbance, and so on are common clinical manifestations of shunt malfunction, some patients may present with odd or unusual presentation. Here, we present a series of patients with previous shunt insertions who presented with odd and unexpected clinical manifestations.
Methods
Eight children with shunt malfunction were enrolled in this series. The age, sex, age of shunting, etiology of hydrocephalus and management, post-shunt insertion symptoms/sign, revision surgery, outcome and follow-up were evaluated.
Results
Patients were aged from 1 to 13 years (mean, 6.38 years). There were 5 males and 3 females. The odd presentation associated with shunt malfunction included facial palsy in three children, ptosis in 3 children, and torticollis and dystonia in 2 children. All patients underwent shunt revision except for one patient in whom a new shunt was inserted. Follow-up showed improved clinical presentation in all patients.
Conclusion
In this series, we reported eight patients with unusual signs and symptoms following shunt malfunction that were successfully diagnosed and managed.