bluuebunny/crossref_metadata_2025_split huggingface dataset:
Menstrual Blood–Derived Mesenchymal Stem Cells Encapsulated in Autologous Platelet-Rich Gel Facilitate Rotator Cuff Healing in a Rabbit Model of Chronic Tears
Authors: Song Ya , Li Ping , Xu Yan , Lin Zhangyuan , Deng Zhenhan , Chen Can 
Published: May 2023

Background: Successful management of chronic rotator cuff (RC) tears remains a challenge owing to its limited intrinsic healing capacity and unsatisfactory failure rate. Menstrual blood–derived mesenchymal stem cells (MenSCs) have the potential to differentiate into the chondrogenic or osteogenic lineage. Autologous platelet-rich gel (APG), a gel material derived from platelet-rich plasma (PRP), can be applied as a carrier system for cell delivery and also as a releasing system for endogenous growth factors. Purpose: To investigate the effect of human MenSCs encapsulated in APG (MenSCs@APG) on the healing of chronic RC tears in a rabbit model. Study Design: Controlled laboratory study. Methods: After evaluation of the effect of PRP on MenSC proliferation or differentiation, the stem cells were encapsulated in APG for in vivo injection. Supraspinatus tenotomy from the right greater tuberosity was performed on 45 New Zealand White rabbits. After 6 weeks, these rabbits were randomly allocated to 3 supplemental treatments during supraspinatus repair: saline injection (control [CTL] group), APG injection (APG group), and MenSCs@APG injection (MenSCs@APG group). At week 18, these rabbits were sacrificed to harvest the humerus–supraspinatus tendon complexes for micro–computed tomography (CT), histological evaluation, tensile test, and MenSC tracking. Results: In vitro results showed that APG can stimulate MenSC proliferation and enhance chondrogenic or osteogenic differentiation. In vivo results showed that APG can act as a carrier for delivering MenSCs into the healing site, and also as a stimulator for enhancing the in vivo performance of MenSCs. Micro-CT showed that bone volume/total volume and trabecular thickness of the new bone in the MenSCs@APG group presented significantly larger values than those of the APG or CTL group ( P < .05 for all). Histologically, compared with the CTL or APG group, significantly more mature fibrocartilage regenerated at the healing site in the MenSCs@APG group. A large number of human nuclei–stained cells were observed in the MenSCs@APG group, presenting a similar appearance to fibrochondrocytes or osteocytes. Biomechanically, the MenSCs@APG group showed significantly higher failure load and stiffness than the APG or CTL group ( P < .05 for all). Conclusion: Human MenSCs@APG facilitated RC healing in a rabbit model of chronic tears. Clinical Relevance: Autogenous MenSCs@APG may be a new stem cell–based therapy for augmenting RC healing in the clinic.
Recruitment and Retention Strategies for Parents Experiencing Vulnerability: Lessons from an Online Parenting Program
Authors: Komanchuk Jelena , Letourneau Nicole , Duffett-Leger Linda , Cameron Judy L. 
Published: May 2023

Background Supportive parenting programs can promote parent-child interactions and children's development. However, families experiencing vulnerability (e.g., low socioeconomic status) report barriers (e.g., transportation, distrust of researchers) to research participation, and attrition rates of 40% and higher have been reported in parenting research. In response, we conducted a longitudinal evaluation of a digital parenting program in a major metropolitan centre in western Canada and retained 99% of our sample. Aim Review recruitment and retention strategies employed in the First Pathways study and evaluate associations between sociodemographic (e.g., income) and psychosocial (e.g., parental depression) factors with recruitment and retention strategies. Methods and Findings In collaboration with community agencies, we commenced recruitment of 100 families experiencing vulnerability (e.g., low-income) in June 2021. We utilized strategies to engage staff (e.g., presentations, gift cards, updates) and employed snowball sampling. Families recruited through community agencies were significantly more likely to experience vulnerability (e.g., low income and education, high adverse experiences) compared to families in the snowball sample. We incorporated strategies to minimize participant burden (e.g., choice of online or in-person meetings), promoted rapport (e.g., holiday texts, nonjudgmental environment), incorporated trauma-informed practices (e.g., sensitive inquiry), and demonstrated appreciation for participants’ contributions (i.e., honorarium). Family experiences of vulnerability (i.e., low income, depressive symptoms, adversity) were correlated with higher participant rescheduling. Conclusion Nurses need knowledge of strategies to promote equitable access to research for families experiencing vulnerability. Digital programs with protocols designed to establish rapport, include trauma-informed practices, and minimize participant burden will likely optimize participation and retention.
Non-stoichiometric effect in the superacid-catalyzed polyhydroxyalkylation of biphenyl and 1-propyl isatin
Authors: Cruz-Rosado Alfredo , Romero-Hernández Juan Enrique , Rios-López Marlene , López-Morales Salvador , Cedillo Gerardo , Rios-Ruiz Lucero Mayra , Cetina-Mancilla Enoc , Palacios-Alquisira Joaquín , Zolotukhin Mikhail G , Vivaldo-Lima Eduardo 
Published: May 2023

An experimental study on the superacid-catalyzed polyhydroxyalkylation of biphenyl (A2 monomer) and 1-propyl isatin (B2 monomer) at non-stoichiometric conditions is presented. The produced high-performance polymers were characterized by gel permeation chromatography matrix-assisted laser-desorption/ionization-time-of-flight (MALDI-TOF), nuclear magnetic resonance and diffusion-ordered spectroscopy (DOSY). High molecular weights (Mw > 150,000 Da) and ultra-high molecular weights (Mw ∼ 900,000 Da) are obtained when B2 is used in excess, which agrees with the behavior observed at non-stoichiometric conditions for other superacid catalyzed polyhydroxyalkylations, contrary to the case when A2 is used in excess, where a reduction in molecular weight is obtained, as reported in a previous study from our group. A, B, and M types of linear polymer molecules, as well as C-type cycles, were observed from the MALDI-TOF data.
Diagnostic Dilemma of ANA-negative Pediatric Systemic Lupus Erythematosus in a South Asian Female
Authors: Khan Qaisar Ali , khan Tehmina , Abdi Parsa , Farkouh Christopher , Anthony Michelle , Hadi Faiza Amatul , Iram Sumaira 
Published: May 2023

Background: Systemic lupus erythematosus (SLE) is an autoimmune disorder affecting multiple organs with different degrees of severity. SLE is typically diagnosed based on the presence of antinuclear antibodies (ANA) in the serum. However, seronegative SLE is rare and is diagnosed by clinicians when the patient’s ANA is negative but fulfills other diagnostic criteria. Case report: We report a case of a 15-year-old South Asian female with SLE who had negative antinuclear antibodies yet displayed the typical clinical presentations of photosensitive maculopapular rash, joint pain, alopecia, anemia, and thrombocytopenia. Clinical evaluations in conjunction with lab results were used to establish a diagnosis of ANA-negative SLE. Conclusion: ANA positivity is an entry criterion for SLE; rarely, cases of ANA-negative SLE may present. A typical clinical presentation may help determine the diagnosis in such a scenario. However, still, the physician should rule out immunodeficiency and other systemic illnesses before reaching a diagnosis of ANA-negative pediatric SLE.
Durability of bond between carbon/glass hybrid fiber-reinforced polymer (HFRP) bar and concrete in water
Authors: Xu Gaojie , Yu Yixun , Pan Yunfeng , Li Biao 
Published: May 2023

Glass-fiber-reinforced polymer (GFRP) bars have been widely used as a reinforcement in concrete, and glass fibers are susceptible to reacting with alkali ions in concrete pores. Thus, carbon fibers are wrapped around GFRP bars to produce carbon/glass hybrid fiber-reinforced polymer (HFRP) bars so as to promote the durability of the internal GFRP bars in concrete. This work studies the durability of the interfacial bond between GFRP bars/HFRP bars and concrete using pullout tests. The specimens are tested after immersion in distilled water for 0, 150, 255, and 544 days. The failure of the GFRP bar–concrete composite is due to damage to the GFRP bar, while the HFRP bar–concrete composite fails because of a combination of the scratching of the HFRP bar and the failure of the concrete. Both HFRP and GFRP bars are scratched as the immersion period extends. Further, the strength of the bond between the HFRP bar and concrete is lower than that of the bond between the GFRP bar and concrete, while the bond stiffness of the HFRP bar–concrete composite is higher than that of the GFRP bar–concrete composite. The bond strength of the HFRP bar–concrete and GFRP bar–concrete composites increases in the early immersion periods but decreases after 544 days of immersion in water. The retention of the bond strength of the HFRP bar–concrete composite is superior to that of the GFRP bar–concrete composite. The carbon fiber coat is proven to improve the durability of the bond between HFRP bars and concrete in water. A formula for predicting the bond strength of HFRP bar–concrete and GFRP bar–concrete composites in water is also developed. Finally, the modeling results demonstrate that the bond strength retention of the HFRP bar–concrete and GFRP bar–concrete composite is 76% and 46% after 50 years of service time, respectively.
Exploring the benefits of nature-based interventions in socio-economically deprived communities: a narrative review of the evidence to date
Authors: Harrison H , Burns M , Darko N , Jones C 
Published: May 2023

Aim: Socio-economic deprivation encompasses the relative disadvantage experienced by individuals or communities in relation to financial, material or social resources. Nature-based interventions (NBIs) are a public health approach that promote sustainable, healthy communities through engagement with nature and show potential to address inequalities experienced by socio-economically deprived communities. This narrative review aims to identify and evaluate the benefits of NBIs in socio-economically deprived communities. Method: A systematic literature search of six electronic publication databases (APA PsycInfo, CENTRAL, CDSR, CINAHL, Medline and Web of Science) was conducted on 5 February 2021 and repeated on 30 August 2022. In total, 3852 records were identified and 18 experimental studies (published between 2015 and 2022) were included in this review. Results: Interventions including therapeutic horticulture, care farming, green exercise and wilderness arts and craft were evaluated in the literature. Key benefits were observed for cost savings, diet diversity, food security, anthropometric outcomes, mental health outcomes, nature visits, physical activity and physical health. Age, gender, ethnicity, level of engagement and perception of environment safety influenced the effectiveness of the interventions. Conclusion: Results demonstrate there are clear benefits of NBIs on economic, environmental, health and social outcomes. Further research including qualitative analyses, more stringent experimental designs and use of standardised outcome measures is recommended.
Thrombectomy for Anterior Circulation Stroke in a Witnessed Late Time Window Versus Early Time Window
Authors: de Castro-Afonso Luís H. , Fornazari Vitor R. , Machado João P. , Nakiri Guilherme S. , Abud Thiago G. , Monsignore Lucas M. , Pontes-Neto Octávio M. , Abud Daniel G. 
Published: May 2023

Introduction Thrombectomy is the standard treatment for anterior circulation stroke due to large vessel occlusions in a late time window (6 to 24 hours) for patients selected based on perfusion imaging. Most patients treated in late time window studies presented as unwitnessed or wake-up strokes. Whether patients presenting with unwitnessed stroke have an actual time window greater than 6 hours is unclear. The aim of this study was to assess the outcomes of thrombectomy in the treatment of patients presenting with anterior circulation large vessel stroke in an actual late time window of more than 6 hours. Methods This single-center registry of thrombectomy in the treatment of stroke caused by anterior circulation large vessel occlusions (LVOs) included 430 patients treated between 2011 and 2019. Patients were divided into 2 groups: an early time window (≤ 6 hours) group and a late time window group (> 6 hours). Results Outcomes of the early and the late time window groups, respectively, were recanalization of 86.8% vs 82.7% ( P = .29), symptomatic intracranial hemorrhage of 8.2% vs 5.7% ( P = .40), good clinical outcome of 45.4% vs 41.3% ( P = .46), and mortality of 20.2% vs 25% ( P = .30) at 3 months. Conclusions Thrombectomy for anterior circulation large vessel occlusions after 6 hours of symptoms onset seems to be as safe and effective as the standard thrombectomy within 6 hours from symptoms onset, even without perfusion analysis. Randomized trials are needed to confirm these findings.
A staghorn kidney stone or extraskeletal osteosarcoma of the kidney? A case report and literature review
Authors: Emadi Torghabeh Ali , Gharib Masoumeh , Zahed Anaraki Siavash , Rabiei Parisa 
Published: May 2023

Extraskeletal osteosarcoma (ESOS) is a very rare entity among renal malignancies. There are few reports of renal ESOS in the database. Renal ESOS was found to have a high rate of local recurrence and distant metastasis. In most reports, the overall survival of patients was less than 1 year. We present a 51-year-old man who presented with gross hematuria and a clinical diagnosis of a staghorn stone in the left kidney. He underwent radical nephrectomy. The pathologic diagnosis of osteosarcoma was evident.
Risk factors for the long-term incidence and progression of knee osteoarthritis in older adults: role of nonsurgical injury
Authors: Pelletier Jean-Pierre , Paiement Patrice , Dorais Marc , Raynauld Jean-Pierre , Martel-Pelletier Johanne 
Published: May 2023

Background: For one of the most chronic medical conditions, osteoarthritis, uncertainties remain on the impact of injury chronology, the role of repeat injury on the incidence/progression of this disease and the need for knee arthroplasty. Objectives: To explore, in an older adult population, how nonsurgical knee injuries relate to osteoarthritis incidence/progression and the weight of independent risk factors for arthroplasty. Design: A cohort study design evaluates the long-term impact of injuries on knee osteoarthritis outcomes. Methods: Knees with no prior injury ( n = 6358) and with at least one injury ( n = 819) ⩽20 years before study inclusion were from the Osteoarthritis Initiative cohort. Sociodemographic, clinical and structural [X-ray, magnetic resonance imaging (MRI)] data at study inclusion and changes within 96 months were analysed. Statistics included a mixed model for repeated measurements, generalized estimating equations and multivariable Cox regression with covariates. Results: At inclusion, knees with prior injury demonstrated greater incidence and severity of osteoarthritis ( p ⩽ 0.001). At 96 months, there was a greater increase in symptoms [Western Ontario and McMaster Universities Osteoarthritis Index (WOMAC) pain, p = 0.002], joint space width (JSW, p = 0.039) loss, medial cartilage volume loss (CVL, p ⩽ 0.001) and bone marrow lesion size (BML, p ⩽ 0.049). Knees with/without injury at inclusion but with new ones over time had a pronounced increase in symptoms (all WOMAC scores, p ⩽ 0.001), JSW loss, lateral (without) and medial CVL, lateral (without) and medial meniscal extrusion and medial BML (without; all p ⩽ 0.030). Levels of lateral and medial meniscal extrusion (without) and symptoms (with/without; all WOMAC scores, p ⩽ 0.001) were all accentuated with a repeated new injury. Risk factors associated with the highest knee arthroplasty occurrence are new meniscal extrusion and new injury ( p ⩽ 0.001). Conclusion: This study highlights the importance of nonsurgical knee injury in older adults as an independent risk factor for knee osteoarthritis and arthroplasty. These data will be beneficial in clinical practice as they will help identify individuals at greater risk of significant disease progression and worst disease outcomes for a customized therapeutic approach.
Clinicians’ knowledge and attitude towards early mobilization in intensive care units in Ethiopian tertiary hospitals: A multi-centre study
Authors: Dagnachew Tasew Kelemu , Woldegerima Berhe Yophtahe , Yalew Mustofa Salh , Birlie Chekol Wubie 
Published: May 2023

Objectives: To assess clinicians’ knowledge, attitude and associated factors towards patients’ early mobilization in intensive care units in the tertiary hospitals in Northwest Ethiopia. Methods: A multi-centre, cross-sectional study was conducted at the tertiary hospitals in Northwest Ethiopia from April to June, 2022. Data were collected by using self-administered, structured questionnaire; ordinal logistic regression analysis was performed and associations were described in adjusted odds ratios. Results: A total of 304 clinicians were included (response rate of 89.7%). The proportions of poor, fair and good knowledge towards early mobilization in intensive care unit among clinicians were 16.8%, 57.9% and 25.3%, respectively; while that of negative, fair and positive attitude were 16.4%, 60.2% and 23.4%, respectively. Factors associated with better knowledge were being a physiotherapist (adjusted odds ratio = 2.9, confidence interval = 1.2–6.7), having a total work experience >5 years (adjusted odds ratio = 4.6, confidence interval = 1.7–12.1), having an intensive care unit work experience >5 years (adjusted odds ratio = 2.8, confidence interval = 1.1–6.8), previous in-service training (adjusted odds ratio = 1.8, confidence interval = 1.1–3.0) and reading guidelines (adjusted odds ratio = 1.9, confidence interval = 1.1–3.2). Better attitude was associated with in-service training (adjusted odds ratio = 1.9, confidence interval = 1.2–3.1), attending early mobilization courses (adjusted odds ratio = 1.8, confidence interval = 1.1–3.0), presence of mobilization advocators (adjusted odds ratio = 1.7, confidence interval = 1.0–2.8), good knowledge (adjusted odds ratio = 2.6, confidence interval = 1.2–5.8) and fair knowledge (adjusted odds ratio = 2.5, confidence interval = 1.3–4.8). Conclusion: Most of the clinicians had demonstrated fair knowledge and attitude towards early mobilization in intensive care unit. However, there were significant proportion of clinicians who had poor knowledge and negative attitude. We recommended active engagement of physiotherapists and experienced clinicians in intensive care units. Clinicians need to have self-learning habits and attend regular training/courses related to early mobilization in intensive care unit.
CRISPR-cas9 screening identified lethal genes enriched in Hippo kinase pathway and of predictive significance in primary low-grade glioma
Authors: Mijiti Maimaitili , Maimaiti Aierpati , Chen Xiaoqing , Tuersun Maidina , Dilixiati Miershayiti , Dilixiati Yilidanna , Zhu Guohua , Wu Hao , Li Yandong , Turhon Mirzat , Abulaiti Aimitaji , Maimaitiaili Nuerailijiang , Yiming Nadire , Kasimu Maimaitijiang , Wang Yongxin 
Published: May 2023

Abstract
Background
Low-grade gliomas (LGG) are a type of brain tumor that can be lethal, and it is essential to identify genes that are correlated with patient prognosis. In this study, we aimed to use CRISPR-cas9 screening data to identify key signaling pathways and develop a genetic signature associated with high-risk, low-grade glioma patients.

Methods
The study used CRISPR-cas9 screening data to identify essential genes correlated with cell survival in LGG. We used RNA-seq data to identify differentially expressed genes (DEGs) related to cell viability. Moreover, we used the least absolute shrinkage and selection operator (LASSO) method to construct a genetic signature for predicting overall survival in patients. We performed enrichment analysis to identify pathways mediated by DEGs, overlapping genes, and genes shared in the Weighted correlation network analysis (WGCNA). Finally, the study used western blot, qRT-PCR, and IHC to detect the expression of hub genes from signature in clinical samples.

Results
The study identified 145 overexpressed oncogenes in low-grade gliomas using the TCGA database. These genes were intersected with lethal genes identified in the CRISPR-cas9 screening data from Depmap database, which are enriched in Hippo pathways. A total of 19 genes were used to construct a genetic signature, and the Hippo signaling pathway was found to be the predominantly enriched pathway. The signature effectively distinguished between low- and high-risk patients, with high-risk patients showing a shorter overall survival duration. Differences in hub gene expression were found in different clinical samples, with the protein and mRNA expression of REP65 being significantly up-regulated in tumor cells. The study suggests that the Hippo signaling pathway may be a critical regulator of viability and tumor proliferation and therefore is an innovative new target for treating cancerous brain tumors, including low-grade gliomas.

Conclusion
Our study identified a novel genetic signature associated with high-risk, LGG patients. We found that the Hippo signaling pathway was significantly enriched in this signature, indicating that it may be a critical regulator of tumor viability and proliferation in LGG. Targeting the Hippo pathway could be an innovative new strategy for treating LGG.
Sediment Erosion on Pelton Turbines: A Review
Authors: Ge Xinfeng , Sun Jie , Chu Dongdong , Liu Juan , Zhou Ye , Zhang Hui , Zhang Lei , Chen Huixiang , Kan Kan , Binama Maxime , Zheng Yuan 
Published: May 2023

AbstractThe Pelton turbine has been widely used to develop high-head water resources with sediments because of its advantages in life cycle costs. When a flood or monsoon season occurs, the sediment concentration in the river increases suddenly, causing severe erosion to the nozzle, needle, and runner of Pelton turbines. After decades of development, researchers have developed practical engineering experience to reduce the sediment concentration of the flow through the turbine and ensure the safety and efficiency of power generation. Research on the mechanism of sediment erosion, development of anti-erosion materials, and establishment of erosion prediction models have attracted scholarly interest in recent years. Extensive research has been conducted to determine a complete and valuable syndication erosion model. However, owing to the complexity of the flow and wear mechanisms, the influence of specific parameters of erosion and the syndication effect is still difficult to determine. Computational fluid dynamics and erosion monitoring technology have also been evaluated and applied. This paper presents a comprehensive review of the erosion of Pelton turbines, some of the latest technical methods, and possible future development directions.