bluuebunny/crossref_metadata_2025_split huggingface dataset:
Hedonic perception group intervention in adolescent depression: a randomized controlled trial
Authors: Huang Zhen , Tao Mengjun , Li Jinyan , Cheng Jia 
Published: August 2023

Abstract
Evidence from the pleasure perception community has been applied primarily to adults with anxiety and depression. Less is known about the effects of depression and anxiety in adolescents.To determine the efficacy of pleasure perception combined with standard therapy in the treatment of adolescent depression.Participants with ICD-10-diagnosed depression (n = 108) were randomized to receive hedonic perception group therapy combined with standard care (2 weeks, 10 treatments) and the control group received only standard care and were followed up at baseline, 2 weeks (postintervention) and 7 weeks with clinical measures that included depression, anxiety, and somatic symptoms.In the analysis of missing data by maximization, there were significant differences in GAD-7 (P < 0.01), PHQ-9, and PHQ-15 (P < 0.001) between and within groups between the experimental and control groups at 2 and 7 weeks of follow-up. Participants in the experimental group showed better treatment outcomes, and the effect size (Cohen d) of the experimental group was greater than that of the control group. The effective remission rate of the pleasure perception group plus standard treatment group was significantly higher than that of the standard treatment group alone (P < 0.05).The combination of pleasure perception groups and standard treatment is an effective treatment method for adolescent depression, which shows certain clinical application prospects.
Media Matters: Culture Medium-Dependent Hypervariable Phenotype of Mesenchymal Stromal Cells
Authors: Fitzgerald Joan C. , Shaw Georgina , Murphy J Mary , Barry Frank 
Published: August 2023

Abstract
Background
Despite a long history of investigation and sustained efforts in clinical testing, the number of market authorisations for mesenchymal stromal cell (MSC) therapies remains limited, with none approved by the United States Food and Drug Administration (FDA). Several barriers are impeding the clinical progression of MSC therapies, to the forefront of these is a lack of standardised manufacturing protocols which is further compounded by an absence of biologically meaningful characterisation and release assays. A look at clinical trial registries demonstrates the diversity of MSC expansion protocols with variabilities in cell source, isolation method and expansion medium, amongst other culture variables, making it extraordinarily difficult to compare study outcomes. Current identification and characterisation standards are insufficient; they are not specific to MSCs and do not indicate cell function or therapeutic action.
Methods
This work analysed the influence of five widely used culture media formulations on the colony forming potential, proliferation kinetics, trilineage differentiation potential and immunomodulatory potential of human bone marrow derived MSCs (BM-MSCs). The surface marker expression profiles were also characterised using a high-content flow cytometry screening panel of 243 markers.
Results
Significant differences in the biological attributes of BM-MSCs including clonogenicity, proliferation, differentiation propensity and immunomodulatory capacity were revealed in response to the composition of the culture medium. Despite their biological differences, all cell preparations uniformly and strongly expressed the standard positive markers proposed for BM-MSCs; CD73, CD90 and CD105. Immunophenotypic profiling revealed that the culture medium also had a significant influence on the surface proteome, with one third of tested markers exhibiting variable expression profiles. Principal component analysis demonstrated that BM-MSCs isolated and expanded in a proprietary xeno- and serum-free medium displayed the most consistent cell phenotypes with little variability between donors compared to platelet lysate (PL) and fetal bovine serum (FBS)-containing media.
Conclusions
These data suggest that media composition has a highly significant impact on the biological attributes of MSCs, but standard surface marker tests conceal these differences. The results indicate a need for (1) standardised approaches to manufacturing, with an essential focus on defined media and (2) new biologically relevant tests for MSC characterisation and product release.
A k-nearest neighbor query method of privacy protection for university library's self-built featured database based on real time national secret algorithm
Authors: Wu Nan 
Published: August 2023

Abstract
In order to avoid privacy information leakage, a k-nearest neighbor query method of privacy protection for university libraries' self-built featured databases based on the national secret algorithm is studied. Through a parallel clustering algorithm based on grid density and locally sensitive hash functions, hash quantization processes the original dataset in the self-built featured database to obtain average bucket clustering results; Based on the average bucket label, a secure index structure for a one-way dictionary is established to generate a key for encrypting and decrypting the subset of data managed in each average bucket through the SM4 algorithm and SM2 algorithm in the hybrid national security algorithm. Using the secure index structure of a one-way dictionary and the key generated by the national secret algorithm, it can quickly search for k-nearest neighbors of privacy protection and efficiently add, delete, and modify data in the featured database built by university libraries. The experiment proves that this method can effectively encrypt and decrypt the data in the featured database built by university libraries, and achieve the k-nearest neighbor query of privacy protection for the featured database; In different data dimensions, the k-nearest neighbor query efficiency of this method is faster; For different databases, the maximum query inflation of this method is around 9.1, which is within a reasonable range, that is, the security of k-nearest neighbor queries is better.
Stress-resilience in Parkinson's disease: predictors and associations with symptom progression
Authors: Heide Anouk van der , Dommershuijsen Lisanne , Puhlmann Lara , Kalisch Raffael , Bloem Bastiaan , Speckens Anne , Helmich Rick 
Published: August 2023

Abstract
Background: People with Parkinson’s disease (PD) are very sensitive to psychological stress, but not everybody reacts the same to adversity. The ability to maintain mental health despite adverse events has been conceptualized as stress-resilience. It is unclear whether stress-resilience in PD is predominantly determined by dopamine deficiency, psychosocial factors, or both. In animal models of PD, chronic stressors accelerate disease progression, but evidence in humans is lacking.
Objectives: (1) Distinguish stress-reactive from stress-resilient PD patients, (2) identify predictors for stress-resilience (resilience factors), and (3) compare symptom progression between stress-reactive and resilient patients.
Methods: We conducted a longitudinal survey in Personalized Parkinson Project participants (N=350 PD). The COVID-19 pandemic was used as a model of a stressor that was aligned in time for the entire cohort. COVID-19-related stressors, perceived stress, and severity of PD symptoms were assessed at 11 timepoints (April-October 2020). In 322 patients, both pre-COVID and in-COVID objective clinical assessments were available. We quantified individual stressor-reactivity as the residual between actual and predicted perceived stress relative to COVID-19-related stressors, and modeled trajectories of stressor-reactivity across timepoints. We explored pre-COVID predictors of 6-month average stressor-reactivity and tested whether high/low average stressor-reactivity was prospectively associated with one-year clinical progression rates.
Results: Latent class trajectory models distinguished patients with high (N=227) or low (N=123) stressor-reactivity. Pre-existing anxiety, rumination and non-motor symptom severity predicted high stressor-reactivity (risk factors), whereas quality of life, social support, positive appraisal style and cognitive abilities predicted low stressor-reactivity (resilience factors). PD-specific factors, such as disease duration, motor symptom severity and levodopa use, did not predict stressor-reactivity. During the pandemic, motor symptom progression was not accelerated, but depressive symptoms increased specifically in stress-reactive patients.
Conclusion: High stressor-reactivity in PD relates to non-motor symptoms and psychosocial factors, rather than to PD-specific factors. Stressor-reactivity is associated with larger increases in depression but not motor symptoms during follow-up.
Melatonin relieves the S-nitrosylation of plasma membrane H+-ATPase 2 to enhance saline-alkali tolerance in tomato
Authors: Gong Biao , Wei Jin-Wei , Liu Minghui , Cao Bili , Shan Qing , Liu Xiaoqian , Liu Wei , Shi Qinghua , Liu Derui 
Published: August 2023

Abstract
Soil salinity-alkalinity is a global problem with special relevance to land resources. Although melatonin confers multiple tolerances to plants, its role in saline-alkali tolerance remains unclear. Here, we found that excess nitric oxide (NO) accumulation accounts for saline-alkali stress by genetically manipulating the NO metabolic enzyme of S-nitrosoglutathione reductase (GSNOR). A caffeic acid O-methyltransferase (COMT) was identified to enhance melatonin synthesis and NO scavenging to improve saline-alkali tolerance. As a target protein participating in saline-alkali tolerance, plasma membrane H+-ATPase 2 (HA2) was identified to be S-nitrosylated at Cys206. Further, the S-nitrosylation of HA2 was induced by saline-alkali stress, and was aggravated by GSNOR-knockdown, but was alleviated by COMT-overexpression. The S-nitrosylation of HA2 at Cys206 suppressed its interaction with 14-3-3 protein 1 (TFT1), culminating in the inhibition of its enzymatic activity and saline-alkali tolerance. Under physiological status, melatonin and NO jointly act as a redox switch of HA2 to regulate root H+ and Na+ efflux to affect saline-alkali tolerance. Thus, the COMT-, GSNOR-, and HA2-transgenic grafting significantly improved tomato productivity in natural saline-alkali soil. Together, this study unravels the molecular basis of the melatonin-NO-HA2 module and provides effective solution to agricultural applications.
Identifying internal distributions and multi-scenario simulation of ecosystem service value in Liaohe basin based on Geodetector and PLUS model
Authors: Jia Changgeng , Wei Chaoxiang , Luo Kunyu , Li Sihui , Fan Yu 
Published: August 2023

Abstract
Ecosystem services value (ESV) can assess the level of basin ecological restoration and provide a basis for ecological management decision-making. This study object selects Liaohe River Basin (LHB), a typical basin in Northeast China. The ecosystem service value variation caused by changes in land use cover and the spatial distribution characteristics was evaluated by employing the benefit transfer method, which is based on data sets for land use over 2000–2020. Meanwhile, geographical detector was employed to investigate the impacts and interactions of various factors driving the ESV, and predicted future changes in land and ESV by PLUS model. The results showed the following: 1) LHB land transformations mainly concentrated in the transformation between grassland, farmland and forestland. ESV in LHB decreased and then increased between 2000 and 2020 (1224 billion-928 billion-1238 billion), 2) Ecosystem service value exhibited a strong positive spatial autocorrelation, high ESV was mainly distributed in the eastern and western regions of LHB, and low ESV in the central region. 3) The variation in ecosystem service value mainly arises from the human activity intensity index of human factors. 4) In the future, the ecological protection priority scenario can improve the ESV in LHB, and the natural development priority and economic development priority scenario is not conducive to the improvement of ESV. The results show that the ecological restoration effect of the LHB is obvious with the ecosystem service value is significantly improved. In the future, attention should be paid to control human activities and strengthen ecological protection in ESV hotspots.
Genetic structure of brine shrimp Artemia in central-west China: cumulative effects of ancient geological events, climatic changes and recent human activities
Authors: Li Ke , Zhang Rui , Sui Liying , Zhang Chi , Han Xuekai 
Published: August 2023

Abstract
Brine shrimp Artemia is the crucial live food in fish and crustacean larviculture. With the decrease of Artemia resources and the increase in aquaculture demand, it is necessary to conduct Artemia population genetics and to manage this important resource efficiently. In this study, the genetic diversity and distribution pattern of Artemia populations originated from ten inland salt lakes in central-west China were studied using a large number of single nucleotide polymorphisms (SNPs) obtained by high-throughput sequencing. The results showed that Tibetan populations had the highest level of genetic diversity, while Shanxi population had the lowest value. Genetic structure and principal component analysis further revealed these populations can be classified into four geographically and genetically distinct groups, and Tibetan populations were further divided into two subgroups and showed a trend of higher in the west and lower in the east at the level of genetic diversity. In addition, a certain amount of gene flow was observed among these 10 populations, and A. sinica had a unidirectional gene flow to all populations in the eastern Nagri region. Finally, species distribution models indicated that Bio1, Bio4, and Bio12 were the main environmental factors affecting the distribution of Artemia, and climate fluctuations had a strong influence on the distribution of Artemia. The findings revealed Tibetan populations will be further reduced in the future, and it is necessary to adopt a series of measures to strengthen Artemia protection and promote rational exploitation and utilization of this natural resources. This study further improves our understanding of the genetic structure of Artemia in central-west China, and provides certain guidance for the protection of Artemia germplasm resources.
Exhaustion, rather than lack of infiltration and persistence, of CAR-T cells hampers the efficacy of CAR-T therapy in an orthotopic PDAC xenograft model
Authors: Takeuchi Yuta , Wang Yizheng , Sasaki Katsunori , Sato Osamu , Tsuchikawa Takahiro , Wang Linan , Amaishi Yasunori , Okamoto Sachiko , Mineno Junichi , Hirokawa Yoshifumi , Hatanaka Kanako C. , Hatanaka Yutaka , Kato Takuma , Shiku Hiroshi , Hirano Satoshi 
Published: August 2023

Abstract
Background The impressive success of chimeric antigen receptor (CAR)-T therapy in the treatment of hematologic tumors is leading researchers/clinicians to broaden the possible application of this approach to patients with solid tumors, especially patients with pancreatic ductal adenocarcinoma (PDAC). However, CAR-T cell therapy faces disappointing clinical results in solid tumors due to hurdles unique to solid, but not hematologic, tumors, which include restricted trafficking and limited infiltration into tumors, non-durable persistence, and T cell exhaustion. Since the tumor microenvironment composition varies between tumor type meaning CAR-T cells again face a unique set of challenges in each solid tumor, the major hurdle imposed by PDAC on CAR-T cell therapy remains elusive.Methods In this study, we used immunodeficient NOD/Shi-scid, IL-2RγKO Jic mice orthotopically transplanted with human pancreatic tumor cell line, BxPC-3, and transferred with CAR-T cells specific to carcinoembryonic antigen (CEA), a model that allows analysis of quantitative, spatial and functional dynamics of CAR-T cells in tumor tissues unique to PDAC.Results CAR-T cells suppressed tumor growth of orthotopic human PDAC tumors, but only within a limited time window. Contrary to previous studies that demonstrated a limited persistency and infiltration of CAR-T cells in many solid tumors, they persist and accumulated in PDAC tumor tissues, but CAR-T cells become progressively lost ability to control tumor growth. Ex vivo analysis revealed that CAR-T cells that had been recovered at different time points from mice bearing an orthotopic PDAC tumor exhibited a gradual loss of tumor reactivity. This loss of tumor reactivity of CAR-T cells was associated with the increased expression of AMP-activated protein kinase and Mitofusin1.Conclusions The present results demonstrate the potential effectiveness of CAR-T cells in targeting CEA for the treatment of PDAC. However, optimization of CAR-T cells, especially in the areas of resistance to exhaustion is required.
Realization of Collimated Specific Profiles in Rotation-Symmetrical Beam Shaping System with Divergent Light Source
Authors: Tsai Cheng-Mu , Yu Tzu-Chen , Han Pin , Fang Yi-Chin 
Published: August 2023

Abstract
A simple numerical method is proposed for the design of two freeform surfaces, each comprising multiple segmented refractive planes, for generating a collimated beam with a specific irradiance profile in a beam shaping system with a divergent light source. However, in real-world manufacturing, this performance improvement is obtained at the expense of a greater cost and complexity. Accordingly, a second algorithm is proposed which maximizes the number of rays passing through the central regions of the refractive planes in the second freeform surface and hence minimizes the total number of segments required to achieve the same beam shaping performance. The feasibility of the proposed method is demonstrated through the design of two freeform lenses for generating collimated output beams with ring- and triangle-like irradiance profiles, respectively. The experimental results show that the beam profiles are in close agreement with the desired irradiance distributions. In general, the results indicate that the proposed method provides a versatile and efficient approach for achieving the desired collimated profile in beam forming systems with a divergent light source.
A Standardized Rat Model to Study Percutaneous/permucosal Osseointegrated Implants Peri-implantitis
Authors: Liu Xingchen , Deng Shudan , Li Xiyan , Liu Haiwen , Li Zhixin , Wu You , Luo Pu , Zhong Xinyi , Huang Ruoxuan , Liu Runheng , Wu Xiayi , Huang Baoxin , Chen Zetao , Chen Zhuofan , Chen Shoucheng 
Published: August 2023

AbstractBackground:Percutaneous/permucosal osseointegrated implants(POI) are increasingly utilized in clinical practices. However, POI peri-implantitis because of bacterial colonization remains an unsolved problem. With the high incidence rate, distinctive implant characteristic and unique infection pattern, POI peri-implantitis requires a specially designed implant animal model for the researches on the pathogenesis and treatments. Although POI peri-implantitis animal models in forelimb or oral cavity have been utilized in several studies, their high variability in terms of implant site selection, implant design and surgical procedures,etc., have led to unnecessary tissue damage and less effectivity. Herein, a quantitative-analysis-based standardized rat model for POI related research was proposed.Methods:The hard and soft tissue anatomical structures in region anterior to maxillary molar of rat were systematically evaluated by micro-computed tomography (micro-CT) and vascular perfusion to obtain the best surgical site. We then managed to standardize the model by controlling the age, weight, gender of rats and designing implant based on the quantitative-analysis of available volume of bone and soft tissue. Besides,customized instruments andstep-by-step surgical procedures were developed to avoid damage important structures and reduce the variations.Finally, comprehensive assessments including the expression of biomarkers, micro-CT, and histological images were conducted to verify the model.Results:After dissecting the anatomic structures of rat maxilla, we found that placing the implant anterior to the first molar could reduce experimental period and improve animal welfare. We standardized the model by including Sprague-Dawley male rats weighing 450–500g and aged 12–13 weeks. The customized implant size, shape, and a series of matched surgical instruments were appropriately designed. A clear,step by step, surgical process was established. The aforementioned designs ensured the success rate, stability, and replicability of the model.Each validation method confirmed the successful construction of the model.Conclusions:This study proposed a quantitative-analysis-based standardized POI peri-implantitis rat model with improved animal welfare and reliable procedures. This model could provide efficientin vivoinsights to study the pathogenesis and treatmentsof POI peri-implantitis and preliminary screening data for further large animal and clinical trials.
Preliminary utility of group acceptance and commitment therapy for patients with intractable chronic pain presenting to a multidisciplinary pain center in Japan: a single-arm pre- and post-comparative study
Authors: Sakai Mie , Kondo Masaki , Muto Takashi , Sugiura Takeshi , Akechi Tatsuo 
Published: August 2023

Abstract
Background: Multidisciplinary treatments, including psychological approaches such as Acceptance and Commitment Therapy (ACT), are useful for treating chronic pain. However, the few studies investigating the use of ACT on patients with chronic pain in Japan have been limited to case studies and case series. There is a need to develop a Japanese version of a standardized intervention program to expand the use of ACT for patients with chronic pain in Japan. Therefore, the present study examined the preliminary utility of an ACT program for Japanese patients with chronic pain with the ultimate aim of developing a standardized intervention program.
Methods: This was a single-arm, pre-test, and post-test comparative study including 23 patients with chronic pain. Patients attended seven weekly group therapy sessions. Psychological scales were administered before and after the intervention, and results were compared using t-tests.
Results: Following the intervention, participants displayed more positive attitudes towards pain (Chronic Pain Acceptance and Action Questionnaire-pain willingness, d = 0.83), pain catastrophic thinking (Pain Catastrophizing Scale, d = 0.71), receptive attitudes towards pain (Chronic Pain Acceptance and Action Questionnaire-total, d = 0.60), and the degree of pain interference (Brief Pain Inventory-interference, d = 0.55). The intervention had a moderate effect on pain intensity (Brief Pain Inventory-intensity, d = 0.43) and progress along the value (Valuing Questionnaire, d= 0.67).
Conclusions: The preliminary value of the program was demonstrated by its significant effect on pain acceptance. Further efficacy studies comparing groups are required.