bluuebunny/crossref_metadata_2025_split huggingface dataset:
Arginase Activity Inhibition With Thymoquinone Induces a Hybrid Type of Cell‐Death in MDA‐MB‐231 Cell Line
Authors: Bday Jaweher , Souid Moufida , Pires Vivien , Gabbouj Sallouha , Véjux Anne , Lizard Gérard , Hassen Elham 
Published: January 2025

ABSTRACTArginase plays a crucial role in the urea cycle; it also has immunosuppressive and pro‐tumor effects. The present study aimed to assess the effects of arginase inhibition by thymoquinone (2‐Isopropyl‐5‐methyl‐1,4‐benzoquinone), an active compound of Nigella sativa, on cell death in the MDA‐MB‐231 triple‐negative breast tumor cell line. Cell viability assays, Western blot analysis, and flow cytometry analysis were used to characterize oxidative stress and cell death. Our results showed that inhibition of arginase activity with thymoquinone significantly increased intracellular nitric oxide levels and resulted in overproduction of cellular and mitochondrial reactive oxygen species. Reductions in cell viability, cycle arrest, and increased cell death were also observed. Loss of transmembrane mitochondrial potential, activation of caspase‐3, ‐7, and ‐9, cleavage of PARP, condensation and/or fragmentation of the nuclei, suggest that this cell death involved apoptosis. Furthermore, a cytoplasm vacuole formation and an increase in the ratio of [LC3‐II/LC3‐I] suggests a concomitant activation of autophagy with apoptosis. Altogether, the present study highlighted that arginase inhibition with thymoquinone induces a hybrid type of cell death defined as oxiapoptophagy. Thus, arginase inhibition with thymoquinone in the MDA‐MB‐231 cell line could be, in part, involved in the anticancer effect of thymoquinone.
Synthesis and Characterization of Schiff Bases and Their Ag(I) Complexes Containing 2,5,6‐Trisubstituted Imidazothiadiazole Derivatives: Molecular Docking and In Vitro Cytotoxic Effects Against Nonsmall Lung Cancer Cell Line
Authors: Mirghani Ahmed Hamdi , Pehlivanoglu Suray , Alici Hakan , Tahtaci Hakan , Uysal Saban 
Published: January 2025

ABSTRACTIn this study, four novels 2,5,6‐trisubstituted imidazothiadiazole derivative ligands and their Ag(I) complexes were synthesized and characterized using various spectroscopic analysis techniques. First, imidazo[2,1‐b][1,3,4]thiadiazole derivative (3) was obtained from the reaction of 5‐amino‐1,3,4‐thiadiazole‐2‐thiol with benzyl bromide in the presence of KOH in an ethanolic medium. In the next step, the resultant compound reacted sequentially with four substituted phenacyl bromide derivatives (4a–4d) under refluxed ethanol for 24 h to obtain substituted 2‐(benzylthio)‐6‐phenylimidazo[2,1‐b][1,3,4]thiadiazole derivatives (5–8). Compounds (9–12) were obtained by attaching a carbonyl group to carbon number 5 of the imidazothiadiazole group in these compounds with the help of Vilsmeier–Haack reagent. The resultant compounds were reacted in an ethanolic medium to synthesize the novel (13–16) ligands by adding ethylenediamine in a 1:2 molar ratio. The Ag(I) complexes of the resultant ligands were synthesized by mixing silver acetate with the ligands in a dimethyl sulfoxide medium to obtain (17–20) complexes. All the synthesized compounds were analyzed using FTIR, 1H NMR, 13C NMR, mass spectroscopy, magnetic susceptibility, ICP‐OES, and thermogravimetric analysis techniques. The study also investigates the in vitro cytotoxic effect of the ligands and complexes on A549 (nonsmall cell lung cancer) cells using the MTT assay and shows that the 13, 15, and 16 ligands, together with their complexes, exhibit potent cytotoxicity. In addition, in silico molecular docking simulations were conducted both to support the in vitro cytotoxicity experiments and to ascertain the active binding sites and interactions of the ligands and complexes on the EGFR receptor. The result indicates that ligands and complexes may serve as promising candidates for further investigation as anticancer agents.
Safety and Efficacy of DPMAS in Patients With Acute‐on‐Chronic Liver Failure Based on Different Platelet Count Levels
Authors: Niu Lingyun , Zhou Jiamei , Zhao Ninghui , Zhang Aiqing , Ye Qing , Lian Jia , Xu Baiguo , Wen Jing , Yao Jia , Han Tao 
Published: January 2025

ABSTRACTTo investigate the effect of different platelet (PLT) counts on the safety and efficacy of the double‐plasma molecular absorption system (DPMAS) in patients with acute‐on‐chronic liver failure (ACLF). A total of 156 patients with ACLF receiving DPMAS were divided into the observed group (40 × 109/L ≤ PLT < 50 × 109/L) and the control group (PLT ≥ 50 × 109/L) according to PLT count level. The safety and efficacy indices of bleeding‐related complications, PLT reduction rate, total bilirubin (TBIL) reduction rate, and 28‐days survival rate after DPMAS were analyzed and compared between the two groups. The incidence of bleeding complications during and after DPMAS in the observed and control groups (14.3% vs. 14.9%, p = 0.922), the decline rate of PLT immediately and 24 h after treatment (0.13 vs. 0.11/0.05 vs. 0.09, p = 0.256/0.161), and the 28‐days survival rate after treatment (76.2% vs. 75.4%, p = 0.923) were not significantly different. The thromboelastogram before DPMAS showed no significant difference in PLT function between the two groups (p > 0.05). Although the TBIL level of the two groups rebounded 24 h after treatment compared with immediately after treatment, it decreased significantly immediately and 24 h after treatment compared with pre‐treatment levels (p < 0.05). There was no significant difference in the rate of decline of serum TBIL immediately after treatment and 24 h after treatment between the two groups (0.33 vs. 0.35/0.14 vs. 0.16, p = 0.193 and 0.653, respectively). DPMAS is safe and effective in patients with ACLF with 40 × 109/L ≤ PLT count < 50 × 109/L.
Prenatal Corticosterone Impacts Nestling Condition and Immunity in Eastern Bluebirds
Authors: Miller Taylor M. , Navara Kristen J. 
Published: January 2025

ABSTRACTExposure of avian mothers to stressful conditions permanently alters offspring behavior and physiology. Yet, the effects of maternal stress on the development of offspring immunity in birds remain unclear, particularly in wild species. We injected Eastern bluebird (Sialia sialis) eggs with either a corticosterone or control solution, then measured the impacts on nestling morphology and two measures of immunity, bactericidal capacity and swelling responses to phytohemagglutinin. Nestlings from corticosterone‐treated eggs had lower condition indices at hatch but quickly caught up to their control counterparts by Day 5 posthatch and until fledging. Corticosterone‐exposed nestlings also mounted smaller swelling responses to phytohemagglutinin, whereas there were no effects on bactericidal capacity. These results indicate that maternal stress can impact offspring immunocompetence, fitness prospects, and potentially their ability to fend off parasites and pathogens.
Transcriptomic Landscape of Polypide Development in the Freshwater Bryozoan Cristatella mucedo: From Budding to Degeneration
Authors: Kvach A. Yu. , Kutyumov V. A. , Starunov V. V. , Ostrovsky A. N. 
Published: January 2025

ABSTRACTColonial invertebrates consist of iterative semi‐autonomous modules (usually termed zooids) whose lifespan is significantly shorter than that of the entire colony. Typically, module development begins with budding and ends with degeneration. Most studies on the developmental biology of colonial invertebrates have focused on blastogenesis, whereas the changes occurring throughout the entire zooidal life were examined only for a few tunicates. Here we provide the first description of transcriptomic changes during polypide development in the freshwater bryozoan Cristatella mucedo. For the first time for Bryozoa, we performed bulk RNA sequencing of six polypide stages in C. mucedo (buds, juvenile polypides, three mature stages, and degeneration stage) and generated a high‐quality de novo reference transcriptome. Based on these data, we analyzed clusters of differentially expressed genes for enriched pathways and biological processes that may be involved in polypide budding, growth, active functioning, and degradation. Although stem cells have never been described in Bryozoa, our analysis revealed the expression of conservative “stemness” markers in developing buds and juvenile polypides. Our data also indicate that polypide degeneration is a complex regulated process involving autophagy and other types of programmed cell death. We hypothesize that the mTOR signaling pathway plays an important role in regulating the polypide lifespan.
Pesticidal Efficacy of Innovative Heterocyclic Tosyl Esters: Design, Synthesis, Biochemical Assessment, and DFT Calculation
Authors: Metwally Heba M. , Abdel‐Latif Ehab , Mostafa Mohamed E. , Said Gehad E. 
Published: January 2025

ABSTRACTDeveloping new pesticide led compounds to minimize the escalating resistance of agricultural pests which raised by the extensive use of agrochemicals is highly desirable. A series of novel heterocyclic tosyl esters was designed, synthesized, and characterized via different spectral analyses. The pesticidal potential of the thiazolidinone, thiazole, thiophene, and chromene derivatives against Aphis gossypii (Glover) and Tetranychus urticae (Koch) was assessed. The effect of the most potent derivatives on the enzyme activity of both tested pests was also examined. Thiazolidinone derivative 4 was the most effective against either pests, exhibiting significant pesticidal activity that was comparable to or superior to that of standard pesticides. The DFT study was performed for the most potent compounds. The results indicate that these recently synthesized compounds could be effective alternatives in pest controlling strategies.
Patient physical violence toward healthcare workers at a US children's hospital
Authors: DePorre Adrienne G. , Richardson Troy , Puls Henry T. , Bernstein Alec M. , Ebbers Rebecca , Nadler Cy 
Published: January 2025

AbstractAn improved understanding of patient‐related violent events toward healthcare workers (HCWs) is a critical step in mitigating patient violence in the pediatric medical hospital setting. Therefore, we sought to describe the timing/setting, potential antecedents to, and management of pediatric patient‐related violence toward HCW. Using our electronic health record (EHR), we performed a retrospective study of patient‐related physical violent events from 2017 to 2022 among youth hospitalized at our free‐standing children's hospital. We identified 144 violent events associated with 75 patients. Most (66.7%) events occurred after a youth was medically cleared for discharge, and most (55%) events were preceded by an aversive experience the youth was trying to avoid. Most (77.1%) youth received medications for de‐escalation, and nearly one‐half (47.9%) experienced mechanical restraints. Our results highlight the challenges hospitals face while caring for youth at risk for behavioral escalations and support the need for both comprehensive in‐patient behavioral health teams.
The Effect of Flexible Flatfoot on the Running Function in School‐Age Children
Authors: Li Yang , Yan Juntao , Li Yiyang , Yu Yan , Lu Xiaoying , Zhang Jin , Jiang Shuyun 
Published: January 2025

ABSTRACTFlexible flatfoot is common among school‐age children and significantly affects walking efficiency, balance stability, and joint‐movement coordination in children. The demands on the skeletal structure and muscle function are increased during running; however, the impact of a flexible flatfoot on children's running capabilities is unclear. In this study, we aimed to investigate the effects of flexible flatfoot on the running function of school‐age children. Participants with flat feet (n = 28) and typical feet (n = 27) ran on a flat surface at their chosen maximum pace. At the same time, the kinematic and dynamic parameters of their lower limb joints were monitored. A two‐sample statistical analysis assessed the differences in the lower limbs' three‐dimensional kinematic and dynamic parameters during running. The findings revealed a significant reduction in running velocity, stride length, and frequency, and an increased proportion in the support phase (p < 0.05) in children with flexible flat feet. The navicular drop time decreased, whereas the dynamic navicular drop height increased (p < 0.05). A notable decrease in the maximum plantar flexion and eversion torque, power, and power absorption of the ankle joint was observed (p < 0.01). Furthermore, the maximum flexion torque of the knee and hip joints and hip joint power absorption decreased (p < 0.05). The peak ground reaction force in the anteroposterior directions was reduced (p < 0.01). These results indicate that flexible flatfoot can impair the running efficiency of school‐age children and lead to diminished motor stability and reduced propulsive and braking capabilities.
Association between oral feeding versus enteral feeding and cerumen impaction in older hospitalized adults: A retrospective cohort study
Authors: Mansour Asil , Ben‐David Boaz M. , Sasson Ady , Farraj Jalal , Mansour Anwar , Roth Yehonatan , Icht Michal 
Published: January 2025

AbstractBackgroundChewing involves jaw movements that propel cerumen along the ear canal. This mechanism may be reduced in dysphagia, especially for older individuals who are enterally fed. Those patients may be at a higher risk for cerumen impaction and may require longer hospital stays. Examining the relationship between diet type, cerumen impaction, and hospital stay duration was the focus of the present study.MethodsWe performed a retrospective cohort study (not registered) among 114 hospitalized older adults. Data were collected on diet type: (1) oral feeding (individuals fed a solid diet or a pureed diet) or (2) enteral feeding (individuals fed via a feeding tube). The results of an otoscopy that quantified cerumen were recorded, as well as hospital stay duration.ResultsIn a mediation analysis, a hospital stay of >1 month was associated with an increased risk of enteral feeding, which in turn, increased the risk of cerumen impaction. Analysis indicated that the link between longer hospitalization and a more severe level of cerumen impaction was fully mediated by diet type (enteral feeding).ConclusionsEnteral feeding seems to be a risk factor for cerumen impaction, rather than merely hospitalization length, in our sample of geriatric patients. These results highlight the importance of continuous monitoring by ear, nose, and throat specialists, as well as regular auditory assessments for patients who are enterally fed for early detection and treatment of cerumen impaction. Particular attention should be paid to cases of prolonged hospitalization, which is associated with the severity of dysphagia.
Lettuce fortification through vitamin B12‐producing bacteria – proof of concept study
Authors: Pipponzi Sara , Primisser Stefanie , Antonielli Livio , Stefani Emilio , Compant Stephane , Sessitsch Angela , Kostic Tanja 
Published: January 2025

AbstractBACKGROUNDVitamin B12 (cobalamin) can be produced de novo only by certain bacteria and archaea. It plays a crucial role in the health of animals and humans, which obtain it only through diet, mainly from animal products. This study aimed to identify endophytic bacterial strains capable of synthesizing vitamin B12 and enriching edible plants with it as a potential solution for vitamin B12 deficiency in vegetarians, vegans, and people with poor diets.RESULTSAn in silico genome analysis was performed on 66 bacterial genomes, including the reference strain Pseudomonas denitrificans ATCC 13867, a known vitamin B12 producer. The genomes were analyzed using the Rapid Annotations using Subsystems Technology (RAST) server and the MetaCyc database to verify the presence and completeness of the vitamin B12 metabolic pathway. The ability of the strains to produce vitamin B12 was confirmed with a high‐performance liquid chromatography with diode‐array detection (HPLC‐DAD) analysis of pure culture extracts. Eleven strains produced detectable amounts of vitamin B12 under tested conditions. The best performing candidates were further tested for their efficacy in producing vitamin B12 in lettuce grown under sterile conditions on Murashige and Skoog (MS) medium with or without CoCl2 supplementation. Methylobacterium sp. strain P1‐11 produced detectable amounts of vitamin B12 in planta: 1.654 and 2.559 μg per g of dry weight without and with CoCl2 supplementation, respectively.CONCLUSIONThis is the first time a bacterial endophyte was used to produce vitamin B12 in planta, suggesting that bacterial endophytes could be utilized to enhance the nutraceutical values of plant‐based foods. © 2025 The Author(s). Journal of the Science of Food and Agriculture published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.
Effect of ultrasonic‐assisted enzymatic extraction on proso millet bran protein: extraction rate, structural and functional properties
Authors: Li Mengqing , Dang Yueyi , Liu Yangjin , Jiang Fan , Yu Xiuzhu , Du Shuang‐kui 
Published: January 2025

AbstractBACKGROUNDProso millet bran protein (PMBP), derived from agricultural by‐products, possesses high nutritional value, despite its challenging extraction process. The present study proposes an extraction method for PMBP using ultrasound‐assisted cellulase technology (UAE), and optimizes the process parameters. Non‐waxy (N‐PMBP) and waxy (W‐PMBP) PMBPs, extracted through alkaline solubilization and acid precipitation (conventional treatment, CT), served as control groups to assess the impact of UAE on the structure and functionality of PMBP, as well as the distinctions between N‐PMBPs and W‐PMBPs.RESULTSAt an ultrasonic power of 420 W, cellulase concentration of 80 U g−1, enzymolysis pH of 5.0, enzymolysis temperature of 55 °C and enzymolysis time of 3 h, the maximum PMBP extraction rate was achieved at 76.80%. Compared to CT, UAE extraction resulted in enhancements in α‐helix and random content, particle size, surface hydrophobicity (H0) and ξ‐potential value, leading to improved functionality of the PMBPs. Additionally, significant functional and structural disparities were observed between N‐PMBPs and W‐PMBPs. Although no significant difference in molecular weight was detected, each exhibited three major bands. W‐PMBPs exhibited significantly higher beta‐sheet content compared to N‐PMBPs, whereas the reverse trend was observed for α‐helix content. W‐PMBPs displayed strong solubility and water/oil holding capacity compared to N‐PMBPs, albeit with lower emulsifying properties and H0.CONCLUSIONUAE could enhance PMBP extraction, inducing structural and functional changes in PMBPs compared to CT. Significant differences in functionality and structure were observed between N‐PMBPs and W‐PMBPs, providing fundamental data and a theoretical basis for PMBP development and utilization. © 2025 Society of Chemical Industry.
Glycation‐mediated pea protein isolate‐curcumin conjugates for uniform walnut oil dispersion: enhancing oxidative stability and shelf life
Authors: Tan Zhi‐feng , Zhao Guan‐hua , Zhou Da‐yong , Shao Zhen‐wen , Song Liang 
Published: January 2025

AbstractBackgroundTraditional methods for fabricating protein‐polyphenol conjugates have not preserved the structural and functional integrity essential for the food industry effectively. This research introduces an advanced encapsulation methodology designed to overcome these limitations, with the potential to enhance the stability of edible oil matrices significantly, leading to improved preservation techniques and extended shelf life.ResultsGlycated pea protein isolate‐curcumin conjugates (gPPI‐CUR) were developed, demonstrating a marked improvement in the oxidative stability of walnut oil (WO), a proxy for edible oil matrices. Characterized by a Z‐average diameter of 158.37 nm and an encapsulation efficiency of 80.94%, these conjugates demonstrated exceptional performance in reducing lipid oxidation and aldehyde formation. Molecular docking analysis confirmed the formation of robust bonds with curcumin, thereby amplifying antioxidant activity. The uniform distribution of gPPI‐CUR throughout the walnut oil matrix, as validated by confocal microscopy, ensured sustained bioactivity and mitigated the risk of localized oxidation. Electron spin resonance spectroscopy corroborated the superior antioxidant properties of the conjugates, which translated into a substantial 19‐day increase in the shelf‐life of the oil.ConclusionThe gPPI‐CUR conjugates enhanced the oxidative stability of walnut oil significantly, as demonstrated by the increased shelf life and reduced lipid oxidation. This study introduced an effective encapsulation method that improved the stability and extended the shelf life of edible oils, aligning with consumer demands for high‐nutrition food products. The results indicate that the gPPI‐CUR conjugates could serve as a promising antioxidant strategy for food preservation, offering a practical approach to enhance food quality and safety. © 2025 Society of Chemical Industry.
A Comparison of Therapy Adherence for Vocal Fold Atrophy and Chronic Cough
Authors: Schuman Ari D. , Buell Jane B. , Varghese Varsha , Brungardt Madeline E. , Blumhardt Sarah L. , Ongkasuwan Julina , Szymanowski Adam R. 
Published: January 2025

IntroductionTherapy is a common treatment for dysphonia attributable to vocal fold atrophy and chronic cough with the goal of decreasing procedural intervention. We compared the rates of therapy adherence and the factors associated with therapy adherence across groups.MethodsRetrospective chart review at a single institution since 2019. Groups were compared with chi‐square and relative risk.ResultsAcross both diagnoses, 263 patients were identified; 131 (49.8%) with vocal fold atrophy and 132 (50.2%) with chronic cough. 173 patients (66%) were referred to speech therapy (81/131 atrophy, 61.8%; 91/132 cough, 69.7%; p = 0.207). Patients were evaluated in a multidisciplinary setting 30.1% of the time. Among those referred to therapy, patients who underwent their initial evaluation the same day within a multidisciplinary clinic were more likely to complete their course (30.7% vs 10.7%, p = 0.001). This association held on multivariable analysis (relative risk [RR] 2.39, 95% confidence interval 1.13–5.07, p = 0.02). Patients with atrophy and cough had similar therapy completion rates (22.2% vs 11.9%, p = 0.07). Male patients were more likely to complete therapy on multivariable analysis (RR 2.35, 1.16–4.47, p = 0.017). Patients who completed a procedure prior to therapy were not more likely to complete their course (p = 0.52).ConclusionsMultidisciplinary clinic with a speech pathologist and laryngologist is associated with an increased rate of therapy adherence in both vocal fold atrophy and chronic cough, regardless of diagnosis. Pre‐therapy procedural interventions were not associated with increased therapy adherence.Level of Evidence3 Laryngoscope, 2025