bluuebunny/crossref_metadata_2025_split huggingface dataset:
A single-cell atlas of mongolia sheep early embryo
Authors: he Tingyi , guo wenrui , yang guang , su hong , dou aolei , chen lu , ma teng , su jie , liu moning , Le su budeng Ge Ri , qi wangmei , li haijun , mao wei , wang xiumei , li xihe , yang yanyan , song yongli , cao guifang 
Published: January 2023

Abstract
Background
The establishment of different cell types during organogenesis in mouse early embryos has been well studied. However, cell type differentiation and its molecular mechanisms in early embryo development in Mongolian sheep are not well understood.
Results
In this study, we present the first comprehensive single-cell transcriptomic characterization of Ujumqin sheep and Hulunbuir short-tailed sheep at E16. Thirteen major cell types were identified in Ujumqin sheep at E16, and 8 major cell types were identified in Hulunbuir short-tailed sheep at E16. Functional enrichment analysis showed that several pathways were significantly enriched in the notochord cluster, namely, the TGF-beta signaling pathway, Hippo signaling pathway, platelet activation pathway, riboflavin metabolism pathway, Wnt signaling pathway, regulation of actin cytoskeleton, and insulin signaling pathway. In contrast, glutathione metabolism, glyoxylate and dicarboxylate metabolism, citrate cycle, thyroid hormone synthesis, pyruvate metabolism, cysteine and methionine metabolism, thermogenesis, and the VEGF signaling pathway were significantly enriched in the spinal cord cluster. Steroid biosynthesis, riboflavin metabolism, cell cycle, Hippo signaling, Hedgehog signaling, FOXO signaling, JAK-STAT signaling, and Wnt signaling were significantly enriched in the paraxial mesoderm cluster. The notochord cluster, spinal cord cluster and paraxial mesoderm cluster were all strongly related to tail development. Pseudotime analysis demonstrated that mesenchyme can contribute to the notochord in Ujumqin sheep. Further molecular assays showed that the Hippo signaling pathway was enriched in Ujumqin sheep.
Conclusion
This comprehensive single-cell map reveals the contributions of a previously unrecognized signaling pathway and contributes to our understanding of the mechanism of tail development in short-tailed sheep.
Real-time and Efficient EEG Based Driver Drowsiness Detection System with VANET for Safe Driving
Authors: Mohammedi Mohamed , Mokrani Juba , Mouhoubi Abdenour 
Published: January 2023

Abstract
In recent years, the occurrence of vehicular accidents has seen a surge in all societies worldwide. This scourge is considered as one of the most serious issues that society faces, due to the psychological and social damage they cause. This occurrence is mainly due to several reasons, namely, engaging in things while driving, such as eating, writing a text, talking on the cell phone, driving under the influence of alcohol, fatigue, taking medication, etc. Consequently, drowsiness is identified as a factor behind many of these fatalities. For that, it is imperative that all societies pay great attention to the resolution of this issue and try to limit it as much as possible. Therefore, the establishment of a driver drowsiness monitoring system is necessary. Although vehicular communications allow vehicles to disseminate collected data from the vehicle and the driver, existing studies focus primarily on embedded solutions. In this paper, we propose an automated and highly efficient drowsiness detection system using SVM (support vector machine) classifier. The relative power ratio of $\alpha$ wave and $\beta$ wave serve as input to the SVM classifier. As well, the proposed system offer a real-time driver drowsiness detection system with Vehicular Ad hoc NETworks (VANETs) for safe driving. Comprehensive comparisons and implementation results indicate that the proposed system can achieve better accuracy and robustness, and it is feasible to be a subjective detection tool for drowsy level analysis.
Non-Adiabatic Electronic and Vibrational Ring-Opening Dynamics resolved with Attosecond Core-Level Spectroscopy
Authors: Biegert Jens , Severino Stefano , Ziems Karl Michael , Reduzzi Maurizio , Summers Adam , Sun Hung-Wei , Chien Ying-Hao , Gräfe Stefanie 
Published: January 2023

Abstract
Non-adiabatic dynamics and conical intersections play a central role in the chemistry of most polyatomic molecules, ranging from isomerization to heterocyclic ring opening and avoided photo-damage of DNA. Studying the underpinning correlated dynamics of electronic and nuclear wave packets is a major challenge in real-time and many times involves optically dark transient states. We show that attosecond core-level spectroscopy reveals the pathway dynamics of neutral furan across its conical intersections and dark states. Our method measures electronic-nuclear correlations to detect the dephasing of electronic coherence due to nuclear motion and identifies the ring-opened isomer as the dominant product. These results demonstrate the efficacy of attosecond core level spectroscopy as a potent method to investigate the real-time dynamics of photochemical reaction pathways in complex molecular systems.
Root and canal anatomy of mandibular first molars using micro-computed tomography: A systematic review
Authors: AL-Rammahi Hasan M. , Chai Wen Lin , Nabhan Mohamed Shady , Ahmed Hany M. A. 
Published: January 2023

Abstract
Background
A thorough understanding of root and canal anatomy is crucial for successful root canal treatment outcomes. This systematic review aims to explore the published micro-CT based literature about the anatomy of root and canal system in permanent mandibular first molars.
Method
An electronic search was performed on Web of science, PubMed, and Scopus. Published micro-CT journal articles in English that present the root and canal anatomy of permanent 2-rooted mandibular first molars were included. Data on study characteristics, objectives of interest, specifications of the studies, and micro-CT specifications were extracted following the PRISMA statement guidelines. Risk of bias assessment of the included studies was performed using Anatomical Quality Assessment (AQUA) tool. The extracted data were presented in tables or figures to present and synthesise the results. A meta-analysis was performed for the studies related to the prevalence of Vertucci's canal configurations, middle mesial canal (MMC) configurations, and Fan's isthmus types.
Results
Amongst 1358 identified studies, thirty met the inclusion criteria. In terms of the objectives, the selected studies showed high anatomical variability in mandibular first molars. Twenty-two (73%), 25 (83%), and 12 (40%) of the studies reported the population/ethnicity, micro-CT specifications, and ethical approval, respectively. 28 (93%) studies did not disclose the method of sample size estimation. In only 6 (20%) of the studies, the authors had calibrated the assessment approaches. Mostly, a potential risk of bias was reported in domain 1 (objective(s) and subject characteristics) and domain 3 (methodology characterization). Whilst, low risk was reported in domains 2 (study design), 4 (descriptive anatomy), and 5 (reporting of results). Meta-analysis results showed high levels of heterogeneity among the studies related to MMCs (I2 = 86%) and Fan's isthmus (I2 = 87%). As for the root canal configuration, pooled prevalence showed that Vertucci type IV and type I were the most prevalent in mesial and distal root canals, respectively.
Conclusion
There is a considerable heterogeneity in the methodological procedures used to study root canal anatomy in micro-CT studies, however, all involved studies showed that the root canal anatomy of the permanent mandibular first molar is highly variable.
Protocol and registration
The protocol of this systematic review was prospectively registered in the Open Science Framework database (https://osf.io) on 2022-06-20 with the registration number 10.17605/OSF.IO/EZP7K.
Zinc release from mitochondria contributes to MPP+-induced lysosomal disruption and neuronal death
Authors: Kim Yang-Hee , Lee Hyun-Seung , Kang Sun-Ah , Eom Jae-Won 
Published: January 2023

Abstract
Autophagy dysregulation and lysosomal dysfunction are critical in Parkinson’s disease. However, the cause and pathogenic signaling of the lysosomal functional deficiency is unknown. Here, we report on the role of zinc as a link between mitochondrial damage and lysosomal depletion. A mitochondrial toxin, 1-methyl-4 phenylpyridinium (MPP⁺), increased reactive oxygen species (ROS) and intracellular zinc ([Zn2+]i), causing lysosomal membrane permeabilization (LMP) and cell death. Supporting this, antioxidant or zinc chelator significantly reduced MPP⁺-induced LMP and neuronal death, whereas lysosomal protease inhibitors attenuated neuronal death but not ROS and [Zn2+]i. Whereas H₂O₂ toxicity was almost completely attenuated in Metallothionein-3 (MT-3) knock-out (KO) astrocytes, zinc overload- or MPP⁺-induced toxicity increased in MT-3 KO astrocyte cultures, suggesting that MT-3 modulates excessive zinc rather than providing a source of zinc after MPP⁺ treatment. Next, mitochondria-deficient Rho 0 cells were used to determine whether mitochondria are a source of zinc. No increase in ROS, [Zn2+]i, LMP, or MPP⁺ toxicity was observed in Rho 0 cells compared to wild-type cells, suggesting that increased ROS and [Zn2+]i by MPP⁺ originated from mitochondria. Taken together, we suggest that LMP is induced by the release of zinc after mitochondrial damage, eventually leading to neuronal death and lysosomal deficiency. Conduct of future studies will be needed to determine whether zinc is involved in MPP+-induced blocking of autophagic flux and accumulation of α-synuclein.
Innovative City Policy and Water Pollution: Evidence from a Quasi-natural Experiment in China
Authors: He Lingyun , Yuan Enyu , Zhang Yuanmeng , Zhu Zhenghong 
Published: January 2023

Abstract
This study evaluates the impact of China’s innovative city pilot policy on urban water pollution. Based on panel data of 278 cities in China from 2005 to 2018, we employ the difference-in-differences method and find that the policy can significantly reduce water pollution in pilot cities. The lags exist in the effect of innovative city pilot policy, but the effect remains significant in the long term. Heterogeneity analysis shows that the effect is more pronounced for cities in eastern and central regions of China, and the policy is particularly effective among cities at prefecture level. An examination of the underlying mechanisms shows that promotion in urban technological innovation, human capital accumulation and the advancement of industrial structure associated with the innovative city pilot policy may play a role. Our findings indicate that establishing an innovation-driven development model through policy support and enhancing urban innovation level are beneficial to reducing water pollution and achieving green development.
JEL: C33; Q55; R58
A simple self-powered sensor for the detection of D2O and other isotopologues of liquid water
Authors: Zhao Xiaoye , Yang Hanwen , Duley Walter , Zheng Shuo , Guo Tao , Tan Caiwang , Song Xiaoguo , Feng Jicai , Zhou Norman 
Published: January 2023

AbstractWe report the development and evaluation of a simple, inexpensive sensor capable of detecting liquid D2O and other isotopologues of liquid water through the measurement of electrical signals generated from a nano-porous alumina film. This electrical output, consisting of a sharp voltage pulse followed by a separate broad voltage pulse, is present during the application of microliter volumes of liquid. The amplitude and temporal characteristics of these pulses have been combined to enable four diagnostic parameters used for the sensing of D2O and H218O. The sensing mechanism is based on spatially localized variations in the surface potential of alumina, induced by isotopically substituted water molecules, combined with the effect of isotopic composition on charge transfer to the sensor. As a proof-of concept demonstration, a sensing system has been developed that provides real-time detection of liquid D2O in a stand-alone system.
Prevalence and concentration of ochratoxin A in spices: a global systematic review and meta-analysis study
Authors: Safaei Payam , Mohajer Afsaneh , Sadighara Parisa , Ghanati Kiandokht 
Published: January 2023

Abstract
Ochratoxin A is one of the most important toxic metabolites of fungi that can be found in agricultural products. This study aimed to estimate the prevalence and concentration of OTA in spices through meta-analysis. Therefore, online databases including PubMed, Web of Science, and Scopus were screened systematically from 1995 to 2022 to collect the related data. After assessing for eligibility, 36 articles with 1686 samples were included in the study. According to findings, the global pooled prevalence of OTA was counted as 50% (95% CI: 47–52%). Moreover, the highest and lowest concentrations of OTA in spices were noted in paprika (50.66 ng/g) and cinnamon (3.4 ng/g), respectively. The outcome of this meta-analysis can be used for risk assessment model development, aiming to help the government and industries for finding a specific way to reduce the prevalence of OTA spice products.
Multicentric study for estimation of prevalence of microsatellite instability and Lynch syndrome amongst colorectal cancer patients in India
Authors: Sheth Harsh , Jain Abhinav , Shah Mithun , Shah Pankaj , Advani Suresh , Thomas Lidiya , Yagnik Vipul D. , Tank Avinash , Shah Chirag , Thakkar Bhavesh , Bhansali Darshan , Gandhi Manish , Patel Tarang , Patel Natoo , Patel Ashok , Patel Ruchir , Gaadhe Ravindra , Shah Chintan , Burn John , Eswaran Jeyanthy , Patel Chandni , Soni Prachi , Sheth Frenny , Sheth Jayesh , Trivedi Sunil , Ramprasad Vedam , Mannan Ashraf U. 
Published: January 2023

Abstract
Purpose
Colorectal cancer (CRC) is the fifth most common cancer in India, however, there is a paucity of systematically collected data related to its molecular epidemiology, specifically related to tumour microsatellite instability (MSI) and Lynch syndrome prevalence.
Methods
We prospectively recruited 207 unrelated patients who were diagnosed with CRC from whom primary tumour biopsy along with a matched blood sample was obtained. A sequential genetic testing approach for Lynch syndrome detection in colorectal cancer patients in accordance with the UK’s National Institute of Health and Care Excellence’s guideline (DG27) was utilised. Briefly, DNA from tumour biopsies were tested for MSI status followed BRAF V600E testing in samples which showed MSI-high result. Germline testing for the mismatch repair genes was carried in patients who had MSI-high and BRAF V600E negative tumours. Seventeen patients recanted their consent to participate in the study and therefore, results from 190 out of 207 patients is presented here.
Results
Mean age at cancer diagnosis across the cohort was 52.3 years with male to female ratio of 2:1 and 57.3% of the patients had tumours in the descending colon or rectum. MSI-high status was observed in 79 patients (42.6%) and, was inversely associated with age (OR = 0.95, 95% CI = 0.92–0.97, p = < 0.001) and cancers in distal colon and rectum (OR = 0.42, 95% CI = 0.22–0.81, p = 0.009 for distal colon; OR = 0.13, 95% CI = 0.04–0.40, p < 0.001 for rectum). Of these, 76 patients had BRAF V600E negative mutation status (96%) and of these, 48 were diagnosed with Lynch syndrome (63%; MLH1 = 38, MSH2 = 4, MSH6 = 4, PMS2 = 1, EPCAM = 1). The variants c.154del and c.306G > T in the MLH1 gene were most commonly observed across Lynch syndrome patients in our cohort.
Conclusions
This is the first systematic evaluation of the molecular epidemiology of CRC in India. We observe a high proportion of patients with young onset CRC coupled with high prevalence of MSI-high status and Lynch syndrome. The study provides a unique opportunity to explore development of novel Lynch syndrome detection and cancer prevention pathway in Indian healthcare settings.
Use of complementary and alternative medicine in Shanghai outpatients with chronic kidney disease: a cross-sectional survey
Authors: Xiao Yao , Zhang Li , Zhang Jing , Zhou Liuliu , Lu Yeyun , Jiang Yufeng , Zheng Xiaodong 
Published: January 2023

Abstract
Background: Complementary and alternative medicine (CAM) measures are widely used by patients with chronic kidney disease (CKD), but their safety and efficacy have also been questioned. The purpose of this study was to determine the extent of CAM use by patients with CKD and to analyze the CAM-related information.
Methods: This was a cross-sectional study performed in Shanghai. In brief, 491 patients with CKD were studied in three hospitals from June to August 2022. Patients were selected using convenience sampling. The questionnaire was comprised of standard demographic questions as well as questions related to the use of CAM measures.
Results: Most CKD patients (80.58%) reported using CAM therapy, and the most common measures were medicinal herbs (87%) and acupuncture (31%). In general, CAM measures were used to treat co-morbidities (46.89%) and CKD (36.01%). Furthermore, 47.15% of patients reported using CAM therapy for 6 months, and 77.98% of patients did not disclose the use of CAM therapy to their physicians because they were not asked about it (69.77%). Approximately two-thirds of patients reported CAM to have benefits, and side effects were mentioned by 21.5% of patients. CAM therapy did not cause financial burdens for 70% of patients, and they would recommend CAM to relatives and friends.
Conclusion: CAM is widely used by patients with CKD, and medicinal herbs are the most common measure. Patients initiated CAM therapy after receiving information from relatives and friends. Healthcare professionals should be aware of the use of CAM measures to control and prevent adverse effects when combined with conventional medicines and treatments.
Clinical outcomes of surgical treatment of type III laryngotracheoesophageal clefts
Authors: Tan Letian , Xie Yi , Wu Xiufa , Li Qi , Chen Chao 
Published: January 2023

AbstractObjectiveTo describe the clinical outcomes of patients who underwent transcervical repair with a posterior cartilage graft for type III laryngotracheoesophageal cleft (LTEC).MethodsA chart view was performed on patients with type III LTEC between May 2017 and May 2022. Demographic features and surgical outcomes were collected and analyzed.ResultsFive patients were finally included, and all patients survived and thrived postoperatively. Three patients were able to successfully extubate with acceptable voice, and two patients were tracheostomized. Four patients were deemed safe for all consistencies and one was safe for thickened.ConclusionsAn anterior laryngofissure approach to the cleft repair with a posterior cartilage graft is an effective and safe treatment for type III LTEC, whichenables closure of LTEC and reconstruction of cricoid plate in order to avoid tracheoesophageal fistula formation or subglottic stenosis postoperatively. Severe tracheomalacia and GERD are two main causes for surgical failure.
Prognostic significance of pretreatment albumin-bilirubin (ALBI) grade and platelet-albumin-bilirubin (PALBI) grade in patients with small cell lung cancer
Authors: Kut Engin , Menekşe Serkan 
Published: January 2023

Abstract
Small cell lung cancer (SCLC) is one of the most common cancers worldwide. Despite advances in diagnosis and treatment, the prognosis is still poor. There is no effective biomarker other than stage in daily practice. However, in daily practice, patients may have different features and survival times even though they have the same stage. Previously, albumin-bilirubin (ALBI) grade, platelet-albumin-bilirubin (PALBI) grade were used to determine the prognosis of acute-chronic liver failure and acute upper gastrointestinal bleeding in liver cirrhosis. In subsequent studies, they were found to be associated with prognosis in hepatocellular carcinoma (HCC) and other solid cancers.However, the prognostic relationship between ALBI grade, PALBI grade, and SCLC is unknown. Therefore, we conducted this study to examine the relationship between ALBI grade and PALBI grade and prognosis in SCLC patients. Data of 138 patients with advanced SCLC at diagnosis between 2009 and 2020 were analyzed retrospectively. The results of the multivariate analysis were as follows: ALBI grade 1 vs 2, hazard ratio (HR) = 2.905, p < 0.001 for overall survival (OS) and HR = 1.800, p < 0.001 for progression-free survival (PFS); ALBI grade 1 vs 3, HR = 4.340, p < 0.001 for OS and HR = 3.105, p < 0.001 for PFS; PALBI grade1 vs 2, HR = 2.031, p = 0.002 for OS and HR = 1.674, p < 0.001 for PFS; and PALBI grade 1 vs 3 (HR = 4.045, p < 0.001 for OS and HR = 2.972, p < 0.001 for PFS). In conclusion, the ALBI and PALBI grades were determined to be associated with the prognosis of SCLC, and they can be used as easy, inexpensive, and practical markers in determining the follow-up treatment and prognosis of SCLC patients.
The Extra length of stay, costs, and mortality associated with healthcare-associated infections (HCAI) at a referral hospital: a prospective nested case control study
Authors: najafpour zhila , moradi samaneh , cheraghian Bahman , keliddar iman , mombeyni razieh 
Published: January 2023

AbstractBackground:healthcare-associated infections (HCAIs) are one of the real risks in any health system and have a considerable effect on increased morbidity, mortality, and financial burden. The present study aims to investigate the mortality rate, length of stay, and hospitalization cost in patients with and without HCAI.Methods:A prospective cohort study was conducted on 396 Patients with and without HCAI. They were matched with the patients in the same ward and at the same time. This study was done in an educational hospital in Ahvaz with 800 beds for five months. Descriptive analyses were done based on total direct costs, LOS, and mortality rates in patients with and without HCAI. The magnitude of the relationship between risk factors and HCAI was quantified using the odds ratio (OR). Logistic regression was used to calculate the OR.Results:The most common HCAI and microorganisms were UTIs and E. coli. Infected patients had longer hospitalization times (mean 19.58 vs. 7.62, P < 0.05). The mortality rate in cases increased significantly compared with the uninfected group (22% vs. 4.7% P < 0.05). When compared with those who did not develop an infection (control group), infection was associated with significantly higher treatment cost (7399.13±9631.98) (2765.19±2999.33), (P< 0.001).Conclusions:An infection acquired during a hospital stay was associated with higher hospitalization costs, prolonged hospitalization, and a significant increase in the rate of mortality.