bluuebunny/crossref_metadata_2025_split huggingface dataset:
Integrative Korean medicine for recurrent lumbar disc herniation after coronavirus disease vaccination: A case report and literature review
Authors: Koh Ah-Ra , Kim Hyun-Woo , Lee Young-Jin , Jo Hye-Jeong , Chae Go-Eun , Kim Dong-Woo , Ha In-Hyuk , Kim Doori 
Published: January 2025

Rationale:
Many side effects have been associated with the coronavirus disease (COVID-19) vaccine. While most adverse events (AEs) are mild, serious adverse events are occasionally observed in the neurological and musculoskeletal systems. Nevertheless, articles reporting such serious adverse events after COVID-19 vaccination are limited, and only few case reports with detailed descriptions are available in the literature.

Patient concerns:
Herein, we report the case of a 41-year-old male office worker who developed symptoms of recurrent disc herniation 2 days after COVID-19 vaccination; the patient had no other factors that may have caused the recurrence of disc herniation, such as excessively vigorous activities, following vaccination.

Diagnoses:
Consequently, the patient was suspected of having cauda equina syndrome owing to recurrent lumbar disc herniation, and he underwent surgery.

Interventions:
The patient underwent integrative Korean medicine treatment, including acupuncture, pharmacopuncture, and Chuna manual therapy, for 8 months postoperatively.

Outcomes:
After treatment, the patient’s postoperative complications improved; the Numerical Rating Scale score changed from 5 to 1, and the Oswestry Disability Index score changed from 30 to 3. A literature review showed various cases of adverse events related to musculoskeletal inflammation or immune-mediated pathogenesis.

Lessons:
This paper confirmed the possibility that COVID vaccination is related to lumbar disc herniation recurrence and the possibility of integrative Korean medicine as an effective treatment option after lumbar disc herniation surgery.
How cesarean section rates can be reduced through an effective financial strategy: A protocol for systematic review
Authors: Sugiyo Dianita , Kyvernitakis Ioannis , Bahlmann Franz , Brüggmann Dörthe , Al Naimi Ammar 
Published: January 2025

Background:
The incidence of cesarean section (c-section) has been increasing after the introduction of national health coverage. There is potential evidence that unnecessary c-sections can be reduced through an effective financial strategy, which would make it possible to increase health equity in the future. Consistent with global trends, the rate of c-section in Indonesia increased from 1.6% in 1991 to 17.6% in 2017, while the World Health Organization standard rate is 10% to 15%. This study aims to explore and analyze strategies to reduce c-section rates and to report evidence-based research on an effective financial strategy model for reducing these rates.

Methods:
We used a systematic review framework involving electronic databases including PubMed, ProQuest, and ScienceDirect. The following literature search terms were used: “cost-benefit analysis,” “universal health care,” “cost controls,” “health expenditures,” “out-of-pocket expenses,” “c-section,” and “abdominal delivery.” The Joanna Briggs Institute critical appraisal checklist was used to independently assess the methodological quality. The findings were compiled using a meta-aggregation approach to summarize quantitative analysis results potentially based on different methodologies.

Results:
Among 883 database records, 26 studies were retained for full-text review. C-section risk factors, the role of financial system evaluation, and the application of the clinical audit cycles with assessments using Robson classification were discussed in the included papers. Several studies highlighted the crucial function of evaluating reward reimbursement schemes, suggesting that decreased c-section rates and other maternal-neonatal outcomes should be used as indicators.

Discussion:
This study identified an evidence base that suggests using Robson classification in clinical audit cycles to reduce c-section rates and avoid unnecessary c-sections. The other proposals for decreasing the rate were mainly focused on financial and nonfinancial strategies applied nationally and locally in hospital settings.
Primary sclerosing cholangitis with PLKR and UGT1A1 mutation manifested as recurrent bile duct stones: A case report
Authors: Zhou Yijun , Shen Wei , Cui Yusheng , Li Yunjiang , Liu Hong , Bao Jianfeng , Jin Qiaofei 
Published: January 2025

Rationale:
Primary sclerosing cholangitis (PSC) is characterized by idiopathic intra- and extrahepatic bile duct inflammation and biliary fibrotic changes. Recurrent bile duct stones due to PLKR and UGT1A1 mutation is an extremely rare complications of PSC.

Patient concerns:
A 26-year-old male patient complains a history of recurrent yellow skin and urine for over a year.

Diagnoses:
Following dynamic magnetic resonance cholangiopancreatography imaging, colonoscopic manifestation, liver biopsy and whole exome sequencing, the patient was finally diagnosed with PSC - ulcerative colitis with PLKR and UGT1A1 mutation.

Interventions:
Following resolution of the obstruction, a long-term regimen of 1000 mg/d ursodeoxycholic acid in combination with 10 mg/d obeticholic acid to improve cholestasis, 8 g/d colestyramine to facilitate adsorption of excess bile acids and 1.2 g/d rifaximin to prevent biliary tract infection were prescribed.

Outcomes:
The patient’s liver biochemical parameters have improved significantly. His condition is stable and has not undergone liver transplantation at this time.

Lessons:
Close and dynamic detection of the patient’s biliary ductal lesions play an important role in the diagnosis of PSC. In the event of relatively rare biliary complications, attention should be paid to the presence of gene mutation.
Addressing Late-arriving Surgeons in Support of First-case On-time Starts
Authors: Ida Jonathan B. , Schechter Jamie H. , Olmstead John , Menon Archana , Iafelice Mary Beth , Sawardekar Amod , Leavitt Olga , Lavin Jennifer M. 
Published: January 2025

Introduction:
First-case on-time starts (FCOTS) is an established metric of perioperative efficiency, impacting global perioperative throughput. Late-arriving surgeons are a common cause of late operating room (OR) starts. This project reflects a quality improvement effort to reduce late surgeon arrivals by 30% for 24 months and improve FCOTS.

Methods:
A multidisciplinary perioperative leadership team developed clear expectations, including tracking, roles, review processes, and consequences. These were broadly communicated among stakeholders, and feedback was incorporated. A new same-day surgeon-to-surgeon feedback mechanism was instituted for late surgeon arrivals, allowing for surgeon feedback and reiteration of expectations. Results were prospectively tracked for 24 months before and following implementation.

Results:
Late surgeon arrivals decreased by 45%, from 23.6 to 13 per month for 24 months before and following implementation, respectively (P < 0.001). Balancing measures did not see increases for the same periods. FCOTS increased from 66% to 72% postimplementation (P < 0.001). Statistical process control P-charts demonstrated centerline shifts for both metrics.

Conclusions:
Development and communication of a clear framework of expectations, review, and consequences, with ongoing monitoring, clear performance expectations, and timely feedback, can reduce late surgeon arrival and improve FCOTS. Direct and timely communication provided immediate feedback to late surgeons and indicated reporting errors, providing more accurate data on late starts. Consistent policy enforcement is critical for credibility.
Lesion-Network Mapping of Post-Stroke Depressive Symptoms: Evidence from Two Prospective Ischemic Stroke Cohorts
Authors: Ríos Ana Sofía , Temuulen Uchralt , Khalil Ahmed , Villringer Kersten , Ali Huma Fatima , Akdeniz Asli , Grittner Ulrike , Becher Matthias , Rackoll Torsten , Nave Alexander Heinrich , Sperber Pia S. , Liman Thomas , Otte Christian , Endres Matthias , Kufner Anna 
Published: January 2025

AbstractBackgroundPost-stroke depression (PSD) affects up to one-third of stroke survivors, significantly impacting rehabilitation success and quality of life. However, its underlying pathophysiology remains unclear.MethodsWe analyzed two independent, prospective ischemic stroke cohorts (PROSCIS-BNCT01363856and BAPTISeNCT01954797; total N=377) to identify brain regions and networks associated with depressive symptoms post-stroke. Lesion-symptom mapping (LSM) assessed associations between lesion location and depressive symptoms measured via the Center for Epidemiologic Studies Depression Scale (CES-D) up to 12 months post-stroke, while lesion network mapping (LNM) evaluated lesion connectivity with brain networks. We explored correlations between spatial similarity to the LNM-identified network and CES-D scores using linear regression models.ResultsLSM revealed no significant associations between lesion location and depressive symptoms. In contrast, LNM showed that lesion connectivity to brain regions—including the frontal pole, middle and inferior frontal gyri, inferior temporal gyrus, supramarginal gyrus, angular gyrus, frontal orbital cortex, and thalamus—correlated with CES-D scores (r=0.12, p=0.02). These regions overlapped with canonical resting-state networks, such as the frontoparietal (Dice coefficient [DC] = 0.28), salience (DC = 0.27), and default-mode networks (DC = 0.20), as well as a previously published depression circuit (DC = 0.43).ConclusionsLesion location alone was not associated with depressive symptoms post- stroke. However, lesion connectivity analysis revealed associations with brain networks, particularly the frontoparietal, salience, and default-mode networks, suggesting that disruption to these circuits may contribute to the development of PSD up to one-year post-stroke.
Different mechanisms link gain and loss of kinesin functions to axonal degeneration
Authors: Liew Yu-Ting , Voelzmann André , Owens Milli , Day Maureece , Cairns William , Jones Ella , Dajas-Bailador Federico , McCann Sophie , Murphy Thomas , Tortoishell Haydn , Parkin Jill , Bailey David M.D. , Landgraf Matthias , Prokop Andreas 
Published: January 2025

AbstractAxons are the slender, often meter-long projections of neurons that form the biological cables wiring our bodies. Most of these delicate structures must survive for an organism’s lifetime, meaning up to a century in humans. Long-term maintenance and sustained functionality of axons requires motor protein-driven transport distributing life-sustaining materials and organelles to places of need. It seems therefore plausible that loss of motor function can cause axon degeneration; however, also gain-of-function conditions were linked to disorders including motor neuron disease or spastic paraplegia. To understand this phenomenon, we studied ∼40 genetic manipulations of motor proteins, cargo linkers and regulators of reactive oxygen species in one standardisedDrosophilaprimary neuron system. Using axonal microtubule bundle organisation as a relevant readout reflecting the state of axon integrity, we found that losses of Dynein heavy chain, KIF1A/Unc-104 and KIF5/Kinesin heavy chain (Khc) all cause bundle disintegration in the form of chaotically curled microtubules. Detailed functional studies of Khc and its adaptor proteins revealed that losses of mitochondrial or lysosomal transport cause ROS dyshomeostasis, which is a condition that inducing MT-curling in fly and mouse neurons alike. We find that hyper-activated Khc induces the same MT-curling phenotype, not through ROS but directly through enhanced mechanical forces. Studies with loss of Unc-104 and expression of an ALS-linked mutant form of the human Khc orthologue KIF5A suggest that the two mechanisms apply to motors beyond Khc. We discuss a model which can explain the surprising common outcome of both conditions and examine its relevance for understanding motor-linked neurodegeneration.
Human resources and healthcare infrastructure in Peru: A cross-sectional analysis from 2018 to 2024
Authors: Bellido-Boza Luciana , Villarreal-Zegarra David , Valdivia-Miranda Paul 
Published: January 2025

ABSTRACTBackgroundThe healthcare challenges from 2018 to 2024 in Peru focused on resource distribution, infrastructure gaps, and the impact of public policies on equity.ObjectiveTo describe the variations in the spatial distribution of healthcare professionals and available infrastructure in Peru between 2018 and 2024.MethodOur design is observational. The Electronic Information Transfer System of Health Service Provider Institutions (IPRESS) and IPRESS Management Units (SETI-IPRESS) database was used between 2018 and 2024. Our study conducted a spatial visual analysis and a multiple linear regression modeResultsOur study reveals an increase in IPRESS reporting data to SETI IPRESS in Peru, resulting in an increase in reported coverage from 2,255 institutions in 2018 to 3,560 in 2024. Additionally, significant growth has been identified in the average number of total clinics, beds, psychologists, medical technologists, obstetricians, pharmacists, and assistants available within IPRESS between 2018 and 2024. However, the average number of nurses and dentists decreased. Furthermore, a concentration of medical resources and infrastructure is observed in specific geographical areas, such as the departments of Lima and Lambayeque.ConclusionsThis study highlights the importance of health policies and strategies that could increase the number of care centers and improve the quality and equity in the distribution of health resources throughout Peru.
Mesoscale landscaping of the TRIM protein family reveals a novel human condensatopathy
Authors: Singh Hari R. , Sharma Vineeta , Nijssen Jik , Pozniakovski Andrei , Rubin Alexander , Liang Lorrin , Ball David , Hong Sunwoo , Gauntner Victoria , Yu Guanghao , Ranga Arathi , Salant David , Hildebrandt Friedhelm , Hyman Anthony A. , Majmundar Amar J. 
Published: January 2025

ABSTRACTThe mesoscale organization of cells is central to cellular physiology and pathology. Cellular condensates often form via biomolecular phase separation, mediated by intrinsically disordered regions (IDRs) and represent a key mechanism for mesoscale organization. The TRI-partite Motif (TRIM) family of ubiquitin ligases is implicated in diverse cellular functions and disease, yet the role of biomolecular condensation in TRIM family organization remains understudied. Here, we systematically investigate the mesoscale localization of 72 TRIM proteins, revealing that a majority form condensates in distinct cellular compartments. IDR content correlates with dynamic condensate formation, suggesting a critical role in mesoscale organization. Focusing on TRIM8, associated with a neuro-renal disorder, we demonstrate that disease-causing truncations of the TRIM8 C-terminal IDR result in acondensatopathy, characterized by disrupted condensation, proteasomal regulation, and TAK1/NFκB signaling. Functional assays in cellular and animal models link these disruptions to podocyte dysfunction and impaired response to injury. Our findings establish a framework for understandingcondensatopathiesand the mesoscale principles governing TRIM family organization and function.
Communication and teamwork between dental surgeons and dental technologists during fabrication of removable complete dentures in Makerere University Dental Hospital: a qualitative research study
Authors: Nono David , Bagenda Godfrey , Okullo Isaac , Rwenyonyi Charles Mugisha 
Published: January 2025

ABSTRACTBackground and ObjectiveEdentulous patients seek services from dental professionals to replace lost teeth with removable complete dentures (RCDs). The dentist (dental surgeon) and dental technologists are expected to work as a team to fulfill all the expectations of the patient. Effective communication between the dentist and the technologist is important in ensuring that dentures are well-fabricated and fitted, and it also impacts the working relationship between the two professionals. The present study explored communication and teamwork between dental surgeons and dental technologists during the fabrication of RCDs.DesignThe study employed a qualitative design to explore communication and teamwork between dental surgeons and dental technologists. It was conducted using in-depth interviews of dental surgeons and technologists guided by data saturation.SettingThe study was conducted in Makerere University Dental Hospital in Kampala, Uganda.ParticipantsTwenty five participants: 13 dental surgeons and 12 dental technologists were interviewed during data collection.ResultsGenerally, participants reported having a good working relationship, where they closely worked together and supported each other to ensure that patients are satisfied with RCD services. The dental surgeons and technologists used different forms of communication. However, the common form of communication was through the use of dental laboratory request forms on which all details about the procedures and materials were provided by the dental surgeons to dental technologists. To complement the dental laboratory request forms, the use of phone calls and electronic media like WhatsApp messages were equally important in emphasizing instructions and follow-ups to ensure that the work was well executed.ConclusionMost of the participants reported having a good working relationship aimed at offering the best services to the patients. Communication between the dental surgeons and dental technologists was mainly through the use of dental laboratory request forms. Phone calls and electronic media were equally important in emphasizing instructions and follow-ups to ensure that the work was well executed.Strengths and Limitations of this study◆The strength of the present study was that it provided baseline data on communication and teamwork between dental surgeons and dental technologists, and how best they can be improved.◆The study limitations were that the participants were limited to general dental practitioners and no dental consultants were interviewed who probably could have different views. It was also conducted at one site (Makerere University Dental Hospital), which renders the findings to be generalized with caution.
Undergraduate GPA does not predict success in PhD programs for cohorts of MS students at two minority-serving institutions
Authors: Fuse M , Rath K , Riggs B , Esquerra RM , Peterfreund A , Gutierrez C , Bayliss F 
Published: January 2025

AbstractMaster of Science (MS) research training programs funded by organizations such as the NIH, the NSF as well as private corporations represent the potential for significant interventions for student success, especially for marginalized or underrepresented and first-generation students, to bridge the gap between undergraduate studies and doctoral programs. These students often face serious challenges during their undergraduate years, such as navigating unfamiliar academic systems, balancing demanding coursework with work responsibilities, and fulfilling family obligations. These and other systemic pressures can impede their academic progress and opportunities for research experience. We therefore asked how their undergraduate GPA impacted success in the PhD in terms of (i) acceptance, (ii) completion, and (iii) time to degree after participating in a funded MS research training program. We examined data collected at San Francisco State University and California State University, Los Angeles over a 30-year period because they (i) had similar student demographics, (ii) were institutions with strong MS degrees, and (iii) had an infrastructure (established offices) to coordinate various training programs. We found that high GPA did not predict greater success in entering or completing the PhD, or in time to degree. This study, therefore, demonstrates that undergraduates from diverse groups with lower undergraduate GPA levels can benefit from a structured MS research training program in reaching their goal of becoming a PhD scientist. Moreover, it indicates that more holistic approaches in admissions are required, including limiting the use of a GPA below 3.0 as an early filter to eliminate applicants. These MS programs appear to play a crucial role as a bridge to PhD studies and are instrumental in enhancing diversity within STEM fields. We suggest that a strategy for success is to provide infrastructure in the form of a coordinated office housing the training grants as a means of structuring mentorship and professional development for under-represented and first generation students.
Transcriptomic Profiling of Old Age Sarcoma Patients using TCGA RNA-seq data
Authors: Nagarajan Vidhyavathy , Karandikar Shreya S. , Dhevanayagam Mary S.J. 
Published: January 2025

AbstractSarcoma is a rare malignancy with poor prognosis, especially in older patients (≥ 65 years) as seen in our preliminary analysis and some previous studies. Moreover, these patients have limited treatment options due to therapy-associated adverse effects and altered tumor micro-environment, which could be associated with their lower prognosis. Studying the underlying biology that drives cancer progression in these patients will help design personalized therapy and improve outcomes for them. This study aims to analyze TCGA-SARC RNA-seq data for characterizing the transcriptomic profile of older age (OA: ≥ 65 years) compared to younger age (YA: 18-65 years) sarcoma patients. RNA-seq and clinical data of sarcoma patients were acquired from TCGA, and the samples were grouped as OA (≥ 65 years) and YA (18-65 years) patients. Differential gene expression analysis, pathway analysis, transcription factor enrichment analysis, gene-specific survival analysis and network analysis were performed. When comparing the gene expression profiles of the 108 OA and 154 YA patients, significant differentially regulated genes (n=733), transcription factors (n=10), hub genes (n=10) and the pathways that characterize the former were identified. Furthermore, 16 dysregulated genes were found that were significantly associated with a poor prognosis in OA sarcoma patients. In accordance with existing evidence of an altered tumor microenvironment in older-age cancer patients, the identified significant genes are associated with the regulation of certain important tumorigenic pathways such as EMT (epithelial-to-mesenchymal transition), calcium signaling, angiogenesis, ECM (extracellular matrix) degradation, Wnt/β-catenin pathways, suggesting the potential cause for lower prognosis in the OA patients. Thus, these findings pave the way to characterize the OA sarcoma patients which can be validated by multi-omics analysis and clinical studies in the future, in turn providing improved treatment options and survival for the same.