bluuebunny/crossref_metadata_2025_split huggingface dataset:
Tamoxifen resistance induction results in mitoxantrone resistance in MCF-7 breast cancer cells via upregulation of ABCG2 expression
Authors: Ghalehno Asefeh Dahmardeh , Abdi Hakime , Boustan Arad , Jamialahmadi Khadijeh , Mosaffa Fatemeh 
Published: March 2023

Abstract
One of the important barriers in the treatment of breast cancer is the development of tamoxifen resistance. Different mechanisms underlying tamoxifen resistance were identified. In this thesis, we aimed to assess the effect of tamoxifen resistance induction on ABCG2 gene/protein expression level and function in the parent MCF-7 breast cancer cell line and its tamoxifen-resistant (MCF-7/TAMR) cell line.
The ABCG2 mRNA and protein expression were compared in MCF-7 and its tamoxifen-resistant derivative MCF-7/TAMR cells using Real-time PCR and western blot methods, respectively. For investigating the effect of tamoxifen-induced resistance on cross-resistance to other chemotherapy drugs such as mitoxantrone (as a well-known ABCG2 substrate), an MTT assay was used. Flow cytometry was applied to compare ABCG2 pump function between two cell lines using mitoxantrone accumulation assay. ABCG2 mRNA expression was also analyzed in tamoxifen-sensitive (TAM-S) (N=9) and tamoxifen-resistant (TAM-R) (N=9) breast tumor tissues.
Our results indicated that the levels of ABCG2 mRNA, protein, and activity were significantly higher in MCF-7/TAMR cells compared to tamoxifen sensitive cell line and mitoxantrone was less toxic in the resistant cell line. Data further discovered that tamoxifen-resistant cells with high ABCG2 activation had a poor response when given rescue mitoxantrone chemotherapy. ABCG2 was also upregulated in tissue samples obtained from tamoxifen-resistant ER+ breast cancer patients compared to tamoxifen-sensitive patients. Increased expression of the ABCG2 mRNA and protein is a phenomenon that occurs after the emergence of resistance to tamoxifen and can cause cross-resistance to mitoxantrone in MCF-7 breast cancer cells.
Steroidogenic activity of methylated resveratrol analogue 3,4,5,4′-tetramethoxystilbene (DMU-212) in human luteinized granulosa cells in a primary three-dimensional in vitro model
Authors: Józkowiak Małgorzata , Kobylarek Dominik , Bryja Artur , Gogola-Mruk Justyna , Czajkowski Mikołaj , Skupin-Mrugalska Paulina , Kempisty Bartosz , Spaczyński Robert Z. , Piotrowska-Kempisty Hanna 
Published: March 2023

Abstract
Purpose
One of the main functions of granulosa cells (GCs) is the secretion of steroid hormones. Resveratrol is a natural polyphenol, known for its beneficial health effects, such as improving reproductive health. However, its application is limited due to poor bioavailability. Methoxy derivative of resveratrol (DMU-212) was shown to be more lipophilic, and consequently more bioavailable. However, since the addition of methoxy groups to the stilbene scaffold was found to make the molecule insoluble in water, DMU-212 was loaded into liposomes. This study aimed to evaluate how the liposomal formulation of DMU-212 (lipDMU-212) alters estradiol and progesterone secretion of human ovarian GCs in a primary three-dimensional cell culture model.
Methods
DMU-212-loaded liposomes were prepared by thin film hydration followed by extrusion. Cell viability was measured after exposure of GCs spheroids to liposomal formulation of DMU-212 using CellTiter-Glo® 3D Cell Viability Assay. The secretion of estradiol and progesterone was determined using commercial ELISA kits. RT-qPCR was conducted to analyze expression of steroidogenesis-related genes.
Results
lipDMU-212 was found to significantly increase estradiol and progesterone secretion in a dose-dependent manner by enhancing expression of CYP11A1, HSD3B1, CYP17A1, CYP19A1, and HSD17B1 genes. Furthermore, our study suggests that lipDMU-212 increases the FSH activity.
Conclusions
This is the first study to describe the steroidogenic activity of liposomal formulation of DMU-212, possibly through increasing the StAR and CYP19A1 expression. These findings suggest that lipDMU-212 might have a beneficial effect in the treatment of disorders related to estrogen deficiency and hyperandrogenism, such as PCOS.
Antimicrobial mouthwashes as a preventive measure against Covid-19 - a comparative analysis of their effects on human chondrocytes and cartilage
Authors: Cai Xiaoyu , Venkatesan Jagadeesh K , Schmitt Gertrud , Reda Bashar , Cucchiarini Magali , Hannig Matthias , Madry Henning 
Published: March 2023

Abstract
Objectives
Antimicrobial mouthwashes are recommended as a preventive measure against SARS-Cov-2. This study compared the cytotoxicity of octenidine dihydrochloride and chlorhexidine gluconate at different concentrations on human chondrocytes and cartilage.
Materials and methods
Primary cultures of normal adult human articular chondrocytes were exposed to octenidine dihydrochloride (0.001562%, 0.003125%, 0.00625%, 0.0125%, 0.025%, 0.05%, and 0.1%), chlorhexidine gluconate (0.003125%, 0.00625%, 0.0125%, 0.025%, 0.05%, 0.1%, and 0.2%), and control (Dulbecco's modified Eagle medium or phosphate buffered saline) for 30 seconds. Normal human articular cartilage explants were exposed to octenidine dihydrochloride (0.1% versus control) and chlorhexidine gluconate (0.1% versus control) for 30 seconds. The viability of human articular chondrocytes was measured by Trypan blue staining, Cell Proliferation Reagent WST-1, and Live/Dead staining. The proliferation of human articular chondrocytes was measured using the Cell Proliferation Reagent WST-1. The viability of human articular cartilage explants was measured by using Live/Dead staining.
Results
Octenidine dihydrochloride and chlorhexidine gluconate exposure decreased cell viability and proliferation in a dose-dependent manner in primary adult human normal articular chondrocytes. Octenidine dihydrochloride and chlorhexidine gluconate exposure decreased cell viability in human articular cartilage explant cultures.
Conclusion
The degree of toxicity varied between octenidine dihydrochloride and chlorhexidine gluconate, with chlorhexidine gluconate being less toxic than octenidine dihydrochloride at the same concentration. Additionally, both octenidine dihydrochloride and chlorhexidine gluconate evaluation had cytotoxic effects on human articular cartilage. Therefore, dosing for the antimicrobial mouthwash ingredients administration would ideally be determined to remain below IC50.
Clinical relevance
Our study supports the in vitro safety of antimicrobial mouthwashes on articular chondrocytes. The data do not suggest to advise such antimicrobial mouthwashes as a general Covid prophylaxis.
Physical and drug release characteristics of nanoliposomes modified poly(vinyl alcohol) (PVA)/poly(ethylene glycol) (PEG) hydrogels
Authors: Jezeh Atefe Jafari , Entezam Mehdi , Haghiralsadat Bibi Fatemeh , Nikukar Habib , Khonakdar Hossein Ali 
Published: March 2023

Abstract
The main objective of this research is to investigate how curcumin liposomal nanocarriers influence the drug release behaviour of PVA/PEG hydrogels in relation to physico-mechanical properties. For this purpose, optimal nanoliposomes from drug loading and release viewpoints, prepared by the thin-film hydration method, were incorporated into the hydrogel composition. Hydrogel samples were physically crosslinked using the freeze-thaw procedure. According to the atomic force microscopy and field-emission scanning electron microscopy observations, nanoliposomes showed a spherical morphology with an average particle size of 100 nm and narrow size distribution. The X-ray diffraction results revealed that adding nanoliposomes to the hydrogel increases the degree of PVA chains crystallinity, enhances tensile modulus and tensile strength of the hydrogel, while decreasing swelling and dehydration rates. SEM micrographs observation displayed that the porosity in the hydrogel structure in the presence of nanoliposomes increases. Nevertheless, in agreement with physical properties, drug release from nanoliposome-in-hydrogel is slower and more controlled as compared to that from free curcumin hydrogel, especially in the early stages. The MTT assay results indicated that although all hydrogel samples are non-toxic, human foreskin fibroblast cell proliferation on hydrogel in the presence of curcumin-loaded nanoliposomes has improved somewhat.
Development and Effectiveness Evaluation of a Propolis Hydroalcoholic Solution With Glycine in Reducing Microorganisms and Removing Helminth Eggs in Leafy Greens
Authors: Silveira Juliana , Alfredo Natália Almeida Corrêa , Beux Marcia Regina , Leal Diego Averaldo Guiguet , Ferreira Sila Mary Rodrigues 
Published: March 2023

Abstract
The main goal of this study was to evaluate the effectiveness of a propolis hydroalcoholic solution with glycine on the reduction of microorganisms and removal of helminth eggs in lettuce leaves. Three experimental groups were evaluated in triplicate or replicates: microbiological (ME), parasitological (PE), and microbiological and parasitological (MPE). Prior to the experiments, the initial concentration of native microbiota was assessed by counting mesophilic aerobes, molds and yeasts, using conventional microbiological techniques. Lettuce leaves were washed in tap water and subjected to different immersion treatments: in propolis hydroalcoholic solution (PHS), in propolis hydroalcoholic solution at pH 5.5 (PHS2), and in propolis hydroalcoholic solution with glycine (PHS-glycine). In ME, the propolis hydroalcoholic solution combined or not with glycine, was evaluated on the reduction of native microbiota. Residual microbiological contamination was assessed. In the PE, the effect of PHS2 and PHS-glycine was evaluated on the removal of a known number of Ascaris suum eggs on leaves artificially contaminated. In MPE, PHS-glycine was evaluated on the reduction of native microbiota and removal of eggs. A reduction of more than 2 log CFU/g were observed in ME. In PE, PHS-glycine removed 37.22% of eggs. In MPE, the PHS-glycine reduced 3.3 CFU/g mesophilic aerobes, 5.0 log CFU/g fungi, and 35.53% of eggs from vegetable. The results highlight the efficiency of the solution on reducing or eliminating different contaminants in vegetables and reinforce the need for the development of hygiene methods that do not harm human health and are reproducible at household level.
Evaluation of RC Elements Under High-Temperature Conditions with different Locations: Analytical Investigation
Authors: Parthasarathi N. , Satyanarayanan K. S. , Prakash M. , Vishal M. 
Published: March 2023

Abstract
Developments in the field of construction has taken an encompassing transformation in the design of structures. Concrete technology has also seen several advancements with the inclusion of reinforcement materials like steel and iron in them which are called as the (RC) Reinforced Concrete. Amidst several developments in the technology of the structural design for a quality construction, RC structural element failure under higher temperature with load remains as one of the most critical mechanisms to be addressed with the recent advancements. There are several methods and studies that try to rule out such failure mechanism with certain improvised methodologies in the fire resistivity behavior of concrete structures. Nevertheless, only a few studies have concentrated on the interaction between the structural and functional elements in the emergence of fire. Considering this, the present work concentrates on the behavior of RC infilled frames with elements like beam, column and beam column joints under higher temperatures and load for which the ABAQUS finite element software is used. The model is simulated with a transient state analysis and characteristics like deflection, stiffness, principal stress are studied for the RC beam, column and beam column joints with varied temperature and load. The outcome of the simulation shows the effect of load and temperature on RC elements and the respective performance graphs are plotted.
Results of Ultrasonography, Uroflowmetry, Voiding Diary Evaluation in Children With Monosymptomatic Primary Enuresis Nocturna
Authors: Yener Sevim , İlçe Zekeriya 
Published: March 2023

Abstract
Objective
To assess examine the results of urinary system ultrasonography, uroflowmetry and voiding diary for children patients presenting with primary monosymptomatic enuresis nocturna.
Patients and methods
Patients aged 5-17 years with enuresis nocturna were retrospectively analyzed. Urinary system ultrasonography (USG), uroflowmetry (UFM), complete urinalysis and two-day voiding diary were recorded for all patients. Comorbid diseases and surgeries were determined with a detailed history.
Results
A total of 842 patients were identified. History of previous surgery was present in 113 (13.4%) patients. Abnormal USG findings were detected in 117 (13.9%) patients.Urinary system USG findings of 17 patients with urinary tract infection were abnormal. In the two-day voiding diary, the bladder volume was found to be equivalent to the expected bladder volume in 54.2% of the patients, while the bladder volume detected in the first uroflowmetry was low in 65% of the patients.
Conclusion
Although the patients do not have urinary system symptoms other than night urinary incontinence at the time of admission, there may be accompanying pathologies. In addition, we think that a voiding diary measurement that has been properly explained and described to the family may be more reliable in cases where UFM cannot be repeated.
Toward Metal- and Catalyst-free Reactions: Deciphering the substituent and external electric field effects on the Diels-Alder reaction between Ethylene and Nitrosoethylene
Authors: Braga Letícia dos Santos , Bevenino Regina Camargo , Leal Daniel Henriques Soares , Ramalho Teodorico Castro 
Published: March 2023

Abstract
The substituents on the diene and/or dienophile can contribute to the decrease or increase of this energy. However, there are other artifices to catalyze chemical reactions, in particular the Diels-Alder reactions, such as, for example, the application of an electric field on the reaction axis. The existence of an external electric field (EEF), properly oriented, has the potential to further stabilize or destabilize these charge transfer contributors and thus influence the energy. So, the goal of this study was to compare the activation energies of the Diels-Alder reaction between ethylene and nitrosoethylene, using an electric field, Lewis acid (BH3) as catalysts and the influence of substituents. All calculations were performed with Gaussian09 software, the compounds were fully optimized using the restricted Hartree-Fock method with the basis set 6-31G, single-point energies determined with MP3 method and basis set 6-31G. The effects of EEFs were studied using the “Field = M ± N” keyword with F values (F=±0,125 au and ±0,080 au) along the preferred axis. The lowest activation barrier is encountered when the electric field is applied, followed by the use of borane as the Lewis acid.
Characterization of chemoresistant human non-small cell lung cancer cells by metabolic and lipidomic profiling
Authors: Lee Ji-Won , Lee Hwanhui , Chun Yoon Shik , Ahn Junyoung , Moon Jeong Yung , Kim Dae Kyeong , Cho Somi Kim , Choi Hyung-Kyoon 
Published: March 2023

Abstract
Introduction
Lung cancer is one of the most malignant cancers and the leading cause of cancer-related deaths worldwide, while acquired chemoresistance would represent a major problem in the treatment of non-small cell lung cancer (NSCLC) because of the reduced treatment-effect and increased rates of recurrence.
Methods
Real-time PCR and Western blotting were employed for investigating mRNA and protein expression of the glutathione peroxidase (GPX) protein family and multidrug resistance protein 1 (MRP1) in A549 and A549/CR cells. We also employed gas chromatography mass-spectrometry and nano electrospray ionization mass-spectrometry coupled with multivariate statistical analysis to characterize the unique metabolic and lipidomic profiles of chemoresistant NSCLC cells in order to identify potential therapeutic targets.
Results
Reactive oxygen species (ROS) levels were decreased, and mRNA and protein levels of GPX2 and multidrug resistance protein 1 (MRP1) were increased in A549/CR. We identified 87 metabolites and intact lipid species in A549 and A549/CR. Among these metabolites, lactic acid, glutamic acid, glycine, proline, aspartic acid, succinic acid, and ceramide, alongside the PC to PE ratio, and arachidonic acid-containing phospholipids were suggested as characteristic features of chemoresistant NSCLC cells (A549/CR).
Conclusions
This study reveals characteristic feature differences between drug-resistance NSCLC cells and their parental cells. We suggest potential therapeutic targets in chemoresistant NSCLC. Our results provide new insight into metabolic and lipidomic alterations in chemoresistant NSCLC. This could be used as fundamental information to develop therapeutic strategies for the treatment of chemoresistant NSCLC patients.
Conditional knockout of AIM2 in microglia ameliorates synaptic plasticity and spatial memory deficits in a mouse model of Alzheimer's disease
Authors: Ye Lei , Hu Mengsha , Mao Rui , Tan Yi , Sun Min , Jia Junqiu , Xu Siyi , Liu Yi , Zhu Xiaolei , Xu Yun , Bai Feng , Shu Shu 
Published: March 2023

Abstract

Alzheimer’s disease (AD) is the most common neurodegenerative disease, and the underlying mechanisms remain unclear. Synaptic dysfunction is a hallmark pathology of AD and is strongly associated with cognitive impairment in AD. Abnormal phagocytosis by microglia is one of the main causes of synapse loss in AD. Existing studies have revealed that inflammasomes contribute to cognitive deficits in AD. Previous studies have shown that the absent in melanoma 2 (AIM2) inflammasome was upregulated in the hippocampus of APP/PS1 mice. In this study, we identified abnormally increased expression of AIM2 in microglia in an Aβ1-42-induced AD mouse model (AD mice). Conditional knockout of microglial AIM2 rescued cognitive impairment and synaptic dysfunction in AD mice. Excessive microglial phagocytosis of synapses was decreased after knockout of microglial AIM2, which was dependent on inhibiting complement activation. These results suggest that microglial AIM2 plays a critical role in regulating synaptic plasticity and memory deficits associated with AD, providing a new direction for developing novel preventative and therapeutic interventions for this disease.
1,25(OH)2D3 promotes insulin secretion through the classical pyroptosis pathway in vitro and vivo
Authors: zheng yuxuan , zhou zhengyu , wei xun , zhang lewen , Hu yudie 
Published: March 2023

Abstract
Purpose: To study the effect of pyroptosis on pancreatic β cell function in diabetes. And explore whether 1,25(OH)2D3 interferes with the progression of type 2 diabetes through pyroptosis signaling pathway.
Methods: We explored the relationship between pyroptosis and diabetes and the role of 1,25(OH)2D3 in this process. In vivo and vitro, we constructing a diabetes model combined with 1,25(OH)2D3 intervention. Detected the insulin secretion level, the release level of inflammatory factors and the expression level of pyroptosis-related proteins.
Results: In vivo or vitro, we have found that 1,25(OH)2D3 can reduce the level of inflammation, reduce the occurrence of pyroptosis by reducing the expression levels of pyroptosis-related proteins NF-κB, NLRP3, GSDMD, and caspase-1. It can protect pancreatic β cell from T2DM damage and increase insulin secretion. Inhibiting the expression of GSDMD can hinder the progress of cell pyroptosis, reduce the expansion of inflammatory response and protect pancreatic cells from damage.
Conclusion: In the occurrence of T2DM, pancreatic cells are induced by pyroptosis through the classical pathway, resulting in reduced insulin secretion. 1,25(OH)2D3 can be used as a potential adjuvant to inhibit pyroptosis of pancreatic cells through pyroptosis classical signaling pathway, reduce inflammatory response and relieve diabetes symptoms.
Adoption of Response Surface Methodology for Optimization of Benzotriazole Additive in Ester Fluids as Transformer Insulant
Authors: J Amalanathan A , R Sarathi 
Published: March 2023

Abstract
This paper reports the critical investigation on the streaming current of ester fluids towards power transformer. The optimization of benzotriazole (BTA) additive is tested based on the streaming current analysis considering its influence with and without the temperature. From the experimental results, the BTA concentration of 130 ppm on ester fluids observed a negative streaming current. The influence of temperature increases the streaming current of ester fluids. Only a minimal change is noticed in the viscosity of ester fluids with the addition of BTA but to the contradict, an increased dissipation factor is observed. The Response surface methodology (RSM) algorithm is being utilized as an optimization algorithm to identify the permissible level of BTA under transformer standstill and normal operating temperature conditions.
Atopic dermatitis and rheumatoid arthritis: a bidirectional Mendelian randomization study
Authors: Wei Mingjing , Shao Zhengchao , Wan Haoyue , Yang Huixue , Chen Wenqi 
Published: March 2023

AbstractBackgroundThe association between atopic dermatitis(AD) and rheumatoid arthritis (RA) has been reported. However, causality has not yet been established. In the present study, Mendelian randomization (MR) analyzes were used to assess whether atopic dermatitis and rheumatoid arthritis are causally associated.ObjectiveIn the present study, MR analyzes were used to evaluate whether atopic dermatitis and rheumatoid arthritis are causally associated.MethodsWe extracted summary-level data for atopic dermatitis and rheumatoid arthritis. Inverse variance-weighted (IVW) analysis was used as the primary analysis. Alternate methods, including weighted median, MR Egger, MR pleiotropy residual sum and outlier, weighted mode, and leave-one-out analysis, were performed to assess pleiotropy.ResultsNo evidence of an effect of rheumatoid arthritis on the risk of atopic dermatitis was found in inverse-variance weighted (OR = 1.043，95% CI =0.959-1.134，p = 0.328). No evidence of an effect of atopic dermatitis on the risk of rheumatoid arthritis was also found (OR=1.026,95% CI =0.903-1.166, p = 0.691).ConclusionsThe large MR analysis indicated that there is no causal relationship between rheumatoid arthritis and atopic dermatitis.
Fine Resolution Analysis of bacterial communities associated with Neochloris oleoabundans culture and insights into terpenes as contamination control agents
Authors: Molina-Aulestia Denisse Tatiana , de Carvalho Júlio Cesar , Pereira Gilberto Vinícius Melo , Vale Alexander Silva , Carvalho Dão Pedro , Soccol Vanete Thomaz , Soccol Carlos Ricardo 
Published: March 2023

Abstract
Biological contamination is one of the main bottlenecks in microalgae production, reducing quality and productivity and sometimes leading to the complete loss of the cultures. Selecting terpenes can be a pathway toward eco-friendly contamination control in microalgae cultures. This work evaluated the presence of bacterial contaminants in N. oleoabundans cultures through HTS and 16S analysis and their susceptibility to six natural terpenes (α-pinene, β-pinene, limonene, trans-cinnamaldehyde, linalool, and eugenol). The principal phyla identified were Proteobacteria, Bacteroidetes, and Actinobacteria, and based on these data, 89 bacterial isolates of seven genera were obtained (36 Aureimonas sp., 27 Microbacterium sp., 5 Pseudomonas sp., 9 Bacillus sp., 14 Shinella sp., 1 Brevundimonas sp., and 1 Exiguobacterium sp.). The minimal inhibitory concentration of terpenes was 6.25 mg L-1, with a reduction in bacterial counts of most isolates by 79% - 99%.