bluuebunny/crossref_metadata_2025_split huggingface dataset:
Phosphoribosyl modification of poly-ubiquitin chains at the Legionella-containing vacuole prohibiting autophagy adaptor recognition
Authors: Mao Yuxin , Wan Min , Minelli Marena , Zhao Qiuye , Marshall Shannon , Yu Haiyuan , Smolka Marcus 
Published: September 2023

Abstract

Ubiquitination is a crucial posttranslational modification in eukaryotes that plays a significant role in the infection of intracellular microbial pathogens, such as Legionella pneumophila, the bacterium responsible for Legionnaires’ disease. While the Legionella-containing vacuole (LCV) is coated with ubiquitin (Ub), it avoids recognition by autophagy adaptors. In this study, we report that the Sdc and Sde families of effectors work together to build ubiquitinated species around the LCV. The Sdc effectors catalyze canonical polyubiquitination directly on host targets or on the phosphoribosyl-Ub (PR-Ub) conjugated to host targets by Sde. Remarkably, the Ub moieties within the poly-Ub chains are either modified with a phosphoribosyl group by Sde and other PDE domain-containing effectors or covalently attached to other host substrates via Sde-mediated PR-ubiquitination. Furthermore, these modifications prevent the recognition by Ub adaptors, such as p62, and therefore exclude host autophagy adaptors from the LCV. Our findings shed light on the nature of the poly-ubiquitinated species present at the surface of the LCV and provide a molecular mechanism for the avoidance of autophagy adaptors by the Ub-decorated LCV.
Time to death and its predictors among neonates admitted to neonatal intensive care unit with perinatal asphyxia in Public Hospitals in East Wallaga Zone, Western Ethiopia, 2023: A retrospective cohort study
Authors: Garuma Worku , Merdasa Emiru , Fantahun Firehiwot , Kaba Zalalem , Adugna Motuma , Fayera Hunde , Raga Imana , Tolesa Roba 
Published: September 2023

Abstract

Background
Perinatal asphyxia cause babies progressively marked impairment of gas exchange and multi-organ system dysfunction due to limited oxygen flow. It is major cause of newborn deaths in the Sub-Saharan Africa region and it contributes 280,000 deaths a year with 31 per 1000 live births case fatality rate. Even though different initiatives and prevention strategies have implemented, neonatal mortality due to perinatal asphyxia is high in Ethiopia. This study aimed to assess time to death and its predictors among neonates admitted to neonatal intensive care units by perinatal asphyxia in public hospitals of East Wallaga Zone, Ethiopia, 2023.
Method
Hospital based retrospective cohort study design was conducted from July 01 2019 to June 30 2022. Systematic sampling technique was employed. Data was coded and entered into EpiData version 4.6 and exported to STATA version 14. Kaplan Meier survival curve and log rank test was used to estimate the survival probability. Log-log plot and global tests was used to assess proportional hazard assumption. Cox Snell residual was used to assess overall model adequacy. Cox proportional hazards regression model was used to determine predictors of mortality. Hazard Ratios (HR) with 95% confidence intervals was computed and statistical significance was declared when it is significant at 5% at p value < 0.05.
Result
From a total of 519 neonates, 27.36% of them were died. The overall incidence of mortality was 45.54 per 1000 (95%CI: 38.63, 53.68) person days observation with median time to death of 4 days. Living in rural area (AHR = 2.69, 95%CI: 1.67, 4.39), delivered from primiparity mothers (AHR: 1.78, 95%CI: 1.01, 2.92), preterm birth (AHR: 1.76, 95%CI: 1.08, 2.87) and having previous history of abortion (AHR: 1.95 95% CI: 1.23, 3.01) were found to have association with the incidence of mortality.
Conclusion and recommendation
: There was high neonatal mortality rate due to perinatal asphyxia. This mortality was high among neonates those whose mothers live in rural area, primiparity mothers, has previous history of abortion and preterm birth babies. Therefore, health workers should give special emphasis for neonates admitted by perinatal asphyxia.
Social participation and exposure to tuberculosis education: a cross-sectional study of older (≥60 years) domestic migrants in China
Authors: Liu Junping , Cui Yu , Zhang Huanyu , Wang Yanfu , Guan Li , Liu Wei , Liu Zhaoyue , Feng Yajie , Liu Xinru , Wu Lin , Wang Nan , Yang Aiying , Liu Zhixin , Liang Libo , Wu Qunhong , Liu Chaojie 
Published: September 2023

Abstract

Background
Older domestic migrants have a higher risk of infection of tuberculosis or reactivation of latent tuberculosis than other populations in China. This study aimed to assess the exposure level of older domestic migrants to tuberculosis education and its association with social participation.
Methods
Data were extracted from the 2017 China Migrants Dynamic Survey (CMDS). A total of 5,787 study participants over 60 years of age were eligible for this study. Multilevel logistic regression models were established to determine the association between social participation and exposure to tuberculosis education after adjustment for variations in sociodemographic characteristics of the participants and health resources available in their migration destinations. Propensity score matching (PSM) was conducted to calculate the average treatment effect of social participation for the treated (ATT).
Results
Only 28.5% of study participants reported exposure to tuberculosis education in various forms over the past year in their migration destination. Participation in social activities, both membership-based (aOR:1.68, 95% CI:1.46–1.94,) and non-membership-based (aOR:1.66, 95% CI:1.44–1.91) were associated with higher exposure to tuberculosis education after adjustment for variations in covariates. Those who resided in a province with higher prevalence of tuberculosis and higher health expenditure, established a local personal health record, were aware of the essential public health services, had a job, obtained higher levels of education, and self-rated good health were more likely to be exposed to tuberculosis education. The ATT of social participation reached 0.10, indicating an increase of 10% exposure to tuberculosis education in those with active social participation.
Conclusions
Low levels of exposure to tuberculosis education in older migrants are evident in China. Social participation is associated with higher exposure to tuberculosis education.
Effect of traditional Chinese exercise on the treatment of "long-COVID" A protocol for systematic review and meta-analysis
Authors: Yang Wentao , cui hua , Wu Minmin , Cheng Guangyu , cheng weiping 
Published: September 2023

Abstract
Background
Since late 2019, there has been a widespread epidemic of coronavirus disease 2019 (COVID-19), and some patients infected with COVID-19 have developed symptoms of long COVID after acute infection control, severely affecting people's lives and work. Traditional Chinese exercise has a long history, with numerous studies proving its effectiveness in the prevention and treatment of COVID-19. Some previous studies only systematically reviewed the effect of TCE on acute covid infection, and there was not enough evidence to support the effect of TCE on persistent fatigue, dyspnea, and cognitive impairment in patients with long-term covid. The purpose of this study was to evaluate the efficacy of traditional Chinese exercise in patients with long COVID and to provide alternative therapy for long COVID.
METHODS
In this study, literature searches in the Cochrane Library, Embase, Web of Science, PubMed, the Chinese Biomedical Literature Database, the Chinese National Knowledge Infrastructure Database, the Chinese Science and Technology Journal Database, and the WanFang Database will be conducted to include literature related to randomized controlled trials of traditional Chinese exercise therapy for long COVID. Two researchers independently screened, extracted data, and assessed the methodological quality of each included trial using the Physiotherapy Evidence Database (PEDro) scale, followed by the Cochrane Collaboration organizational tool being used to assess the risk of bias for each study. The data captured in this study were statistically analyzed using comprehensive meta-analysis version 3.0 software.
PROSPERO registration number：CRD42023402517
Genome-wide identification, phylogenetic analysis, and expression profiling of ATP-binding cassette transporters in oomycete
Authors: LEI yingxia , ZHANG yan , YANG ruiwu 
Published: September 2023

Abstract
Background： ABC transporters are a special family of membrane proteins and fulfill a multitude of functions. In plants, a few research have found ABC transporters acting as virulence factors in oomycete-plant interactions. However, previous studies have only focused on phylogenetic analysis of ABC transporters in particular species, and a comprehensive analysis has not been carried out. In this study, we examined 21 species genomes of oomycetes for phylogenetic and functional analysis of ABC transporters. Aim to find potential virulence factors in the interaction of plants and oomycetes.
Result：We identified 29~151 ABC transporter proteins in 21 species of oomycetes, and 965 were filtered in a phylogenetic analysis. The number of ABC transporters in biotrophic oomycetes was significantly lower than in hemi-biotrophic and necrotrophic oomycetes, and the number in Phytophthora was the largest. Our analysis demonstrated that most oomycete ABC transporters are highly conserved, while others show remarkable group-specific diversification. The ABCA group III and ABCB half-size II proteins clustered with bacteria, appearing to be derived from horizontal transfer events from bacterial genomes. The ABCG subfamily was the most expanded group in oomycetes. The number of ABCD subfamily members was the smallest and was highly conserved in oomycetes. The structural characteristics of ABC proteins indicate diverse structural types in each of the ABC subfamilies. A heatmap was used to analyze the expression of ABC transporters. ABC transporters were expressed in middle and late stages, which represent biotrophic and necrotrophic phases, with the exception of some ABCB transporters being highly expressed in early stage. This suggests that ABC proteins expressed in middle and late stages may participate in host disease and cause host cell death.
Conclusions: In this study, 965 proteins were filtered in a phylogenetic analysis from 21 species of oomycete representing three different lifestyles. Meanwhile, we also analysis of oomycete ABC proteins provides insight into the diversity of the five subfamilies among the main oomycete lineages. This study presents the first comprehensive overview of the ABC transporter family, and provides an important platform for in-depth analysis of oomycete-plant interactions and a database for identifying pathogenic factors in oomycetes.
Future Rainfall Projection Using Modified Local Intensity Scaling (M-LOCI) Method.
Authors: PATTANAYEK TAPAS , SHAIKH SAFAYAT ALI , MUKHERJEE INDRANIL , BHOWMICK PRASENJIT 
Published: September 2023

Abstract
Downscaling techniques are required because General Circulation Models (GCM) cannot be applied directly to climate change projections for climate change impact assessments. However, there are no definite guidelines for choosing a specific statistical downscaling technique to be applied to a certain application. The results of four separate RCPs (Representative Concentration Pathways), each driven by a different model, are scaled in this chapter to evaluate two statistical downscaling methods. Daily rainfall time series for a future scenario are generated for Dumdum station for the period 2021-50 to 2051-80 under climate change projections. Since the fundamental difference between downscaling methodologies relates to how severe events are produced, attention is paid to alterations of extreme events. However, 8 of the 10 GCMs investigated show that future precipitation occurrences will increase. The uncertainties are partially caused by the variability of the statistical downscaling methods and the projection. This chapter highlights the importance in annual average of different GCMs statistical downscaling methods and comparison between different models and different RCPs of same model for use in different hydrological methods.
Effect of hydraulic retention time and electrode type on the performance of semi-continuous flow microbial fuel cell
Authors: Ullah Zia , Ahmad Irfan , Zeshan  
Published: September 2023

Abstract
Traditional wastewater treatment processes demand high energy and are not economically feasible for domestic wastewater treatment. Microbial fuel cell (MFC) offers an alternative option for wastewater treatment with simultaneous energy recovery by utilizing the electrogenic bacteria and organic matter present in wastewater. This study investigates the performance of MFC for the treatment of domestic wastewater when operated at different hydraulic retention times (HRTs). Two dual-chamber MFCs were used each installed with a different type of anode. One of the anodes was a carbon fiber brush and the other was a graphite rod. Domestic wastewater was utilized as a substrate and the reactors were run in a semi-continuous flow at HRTs of 48, 36, 24, 12, 8, 4 and 2 h under 1000 Ω external resistance. At HRT of 8 h, maximum voltage of 319 mV and 308 were achieved for MFC with carbon fiber brush (CFBMFC) and graphite rod (GRMFC), respectively. Maximum COD removals of 80.3% and 73.9% corresponding to maximum coulombic efficiency of 5.4% and 4.2% were achieved for CFBMFC and GRMFC at an HRT of 48 h respectively. Power curves showed that maximum power density of 77 mW/m2 and 58 mW/m2 was achieved for CFBMFC and GRMFC, respectively.
An empirical study of the Flag Rugby game program to promote Gross Motor and Physical Fitness in 5-6 year old preschoolers
Authors: Shi Zuozheng , Yang Xulin , Zhang Xinru , Zhu Weihua , Dai Yuxin , Guan Xuhan , Xuan Shu 
Published: September 2023

Abstract
Objective: To explore the effects of the Flag rugby game courses on the development of Gross Motor and physical fitness in 5-6 year old preschoolers.
Methods: Using the Flag rugby game courses as an intervention, a total of 56 preschool children aged 5-6 years (57.14% girls) were selected to participate in Class A and Class B through a random sampling method to see the effects of the flag rugby game courses on their Gross Motor and Physical Fitness.
Results: After 12W exercised，In the comparative analysis of subjects' Gross Motor and Physical Fitness test results.We found that the ExG differed significantly(P<0.01) in the test results of Gross Motor and Physical Fitness,and a 2 (group: ExG,ConG)×2 (time: 0w,12w) repeated measures ANOVA was performed and found was interacted significantly (P<0.001) in Total Locomotor Subject Score (F=38.287, p=0.000, ES=0.415),Total Object Control Subject Score(F=164.453, p=0.000, ES=0.753),Total Gross Motor Score (F=192.409, p=0.000, ES=0.781),and found was interacted significant (P<0.05) in Grip strength (F=5.030, p=0.029, ES=0.085),Sitting forward bending (F=6.957, p=0.011, ES=0.114),15m obstacle running(F=4.185, p=0.046, ES=0.072),Walking the balance beam(F=7.504, p=0.008, ES=0.122).In the comparative analysis of the results of Gross Motor tests and Physical Fitness in subjects of different genders.The differences in Total Object Control Subject Score (F=5.200,P=0.027,ES=0.850),Grip strength(F=5.971,P=0.018,ES=0.866),Sitting forward bending (F=5.854,P=0.019,ES=-0.863), and 15m obstacle running (F=4.230,P=0.045,ES=- 0.824) had significant (P<0.05) gender main effects.
Conclusion: The 12-week Flag rugby game program can comprehensively and effectively promote the development of Gross Motor and Physical Fitness in preschoolers aged 5-6 years old, and there are certain gender differences in promoting the development of children's Gross Motor and Physical Fitness, which is in line with the law and characteristics of physical and mental development of children's sports,We propose to incorporate it into the movement curriculum for preschoolers aged 5-6 years to enrich the teaching of children's movement curriculum.
Shenfu Injection Regulates Pyroptosis through the NLRP3/Caspase-1 Pathway to Improve Chronic Heart Failure
Authors: FAN Xingyu , LIAO Xiao-qian , HUANG Shu-min , LI Lin , HU Zhi-xi 
Published: September 2023

Abstract
Objective: The objective of this research is to clarify how Shenfu injection enhances chronic heart failure treatment, specifically by examining its influence on the NLRP3-Caspase-1 pathway.
Methods: The chronic heart failure in rats was induced through subcutaneous administration of isoproterenol. The effectiveness of Shenfu injection was assessed by examining cardiac function using echocardiography, monitoring changes in serum N-terminal brain natriuretic peptide levels, and conducting histological analysis with HE and Masson staining to evaluate modifications in myocardial cell structure and fibrosis. To assess the protein levels associated with the NLRP3/Caspase-1 pathway, cellular pyroptosis, and myocardial ultrastructure, various techniques including Western blotting, GSDMD-N staining, TUNEL immunofluorescence dual staining, and electron microscopy were employed.
Results: Shenfu injection significantly ameliorated cardiac function, myocardial arrangement, and fibrosis in chronic heart failure rats. Significantly, it substantially decreased the protein levels of NLRP3, caspase-1, IL-1β, and IL-18, along with a reduction in cellular pyroptosis positivity (P < 0.05). Under electron microscopy, the morphology appeared normal, with only partial cell membranes exhibiting pore formation.
Conclusion: In summary, our research indicates that the SFI intervention improves cardiac function in the chronic heart failure model induced by isoproterenol, possibly by regulating the NLRP3/Caspase-1 pathway to reduce pyroptosis, suppress inflammatory responses, protect myocardial cells, and maintain the integrity of cellular ultrastructure.
Distinct patterns of proteostasis network gene expression are associated with different prognoses in melanoma patients
Authors: Labbadia John , Wellman Rachel , Jacobson Daniel , Secrier Maria 
Published: September 2023

Abstract

The proteostasis network (PN) is a collection of protein folding and degradation pathways that spans cellular compartments and acts to preserve the integrity of the proteome. The differential expression of PN genes is a hallmark of many cancers, and the inhibition of protein quality control factors is an effective way to slow cancer cell growth. However, little is known about how the expression of PN genes differs between patients and how this impacts survival outcomes. To address this, we applied unbiased hierarchical clustering to gene expression data obtained from primary and metastatic cutaneous melanoma (CM) samples and found that two distinct groups of individuals emerge across each sample type. These patient groups are distinguished by the differential expression of genes encoding ATP-dependent and ATP-independent chaperones, and proteasomal subunits. Differences in PN gene expression were associated with increased levels of the transcription factors, MEF2A, SP4, ZFX, CREB1 and ATF2, as well as markedly different survival outcomes. However, surprisingly, the relationship with prognosis was discordant between primary and metastatic sample types. Our findings reveal that the expression of PN genes demarcates CM patients and highlights several new proteostasis sub-networks that could be targeted for more effective suppression of CM within specific individuals.
"I don't want my marriage to end" - A qualitative investigation of the sociocultural factors influencing contraceptive use among married Rohingya women residing in refugee camps in Bangladesh
Authors: Islam Mansura , Habib Shah Ehsan 
Published: September 2023

Abstract
Background:
The timely provision of comprehensive contraceptive services to Rohingya women is impeded due to a lack of clarity and understanding of their traditional beliefs and cultural frameworks. Recognizing this challenge, our aim is to explore the socio-cultural factors that influence the utilization of contraceptives among married Rohingya women living in the refugee camps of Cox's Bazar, Bangladesh.
Method:
A qualitative study was conducted in two unregistered Rohingya camps (Camp7&14) located in Ukhiya Upazila, Cox's Bazar from January 10th to January 20th, 2022. A total of 14 In-Depth Interviews (IDIs) were administered among married Rohingya refugee women of reproductive age (15-49), and 16 Key Informant Interviews (KIIs)were conducted with stakeholders involved in reproductive healthcare provision. The selection of participants was executed through purposive sampling. All interviews were conducted in the local language, recorded, transcribed verbatim, and subsequentlytranslated into English. The data were analyzed using NVivo (Version 11), and the analysis process followed the Neuman three-phase coding system.
Results:
Five broad themes were identified:Sociocultural expectations and values attached to births, power imbalances within marital relationships, the role of religious beliefs, fear of side effects, and misperceptions about contraception. Having a larger number of children is viewed positively as it is believed that children play a crucial role in preserving the lineage and contributing to the growth of the Islamic population. Despite expressing an inclination towards contraception, the disapproval of husbands becomes a significant barrier for women. Defying their husbands' wishes can result in instances of intimate partner violence (IPV) and even marriage dissolution within the camps. Moreover, the fear of side effects, such as a particular method would cause infertility, discourages women from using contraception. Many of these fears stem from myths, misconceptions and mistrust in the existing medical system.
Conclusion:Addressing the socio-cultural barriers that prevent women from using modern contraception will have important public health implications. These findings can support policymakers, civil society organizations, and health providers in crafting culturally sensitive programs and educational interventions. These efforts can aid Rohingya refugee women in surmounting the identified barriers to contraception.
Woody plants diversity and the associated ecosystem service across three contrasting forest management regime in Southwest Ethiopia
Authors: Kebebew Zerihun , Ozanne Claire 
Published: September 2023

Abstract

Coffee management intensification has simplified the stand structure and composition of woody plants in southwest Ethiopia. It is believed that a change in woody plant diversity could results in loss of ecosystem service. Nevertheless, information on the effect of coffee management intensification on ecosystem services is limited. The study aim to give an answer for whether forest modification to coffee agroforest brings about a loss in ecosystem services in southwest Ethiopia. The specific objectives were 1) to examine woody plant diversity across contrasting forest management regime 2) to explore changes in ecosystem services focusing on forest products 3) to examine the relationship between woody plants diversity and ecosystem services as perceived and used by local people. Mixed approaches were employed to collect the data. The forest was divided into three contrasting management regimes. Vegetation data were collected from 189 plots. Ethnoecological approach was employed to assess ecosystem services. Perceived local ecosystem services were collected from 136 individuals. The relationship between ecosystem services and diversity were estimated based on the use value approach. The result showed that there is a positive relationship between the diversity and use value of woody plants across the three forest management regimes. Coffee management intensification simplify both the stand structure, woody plant composition and ecosystem services of the forest. Plant use value increases towards coffee agroforest. Coffee agroforest can serve as repository of diversity and ecosystem services in southwest Ethiopia.