bluuebunny/crossref_metadata_2025_split huggingface dataset:
Genome-wide identification and analysis of HECT E3 ubiquitin ligase gene family in Saccharina japonica and a comparative analysis with five other algae
Authors: Huang Xiang , Liu Yi , Wu Yukun , Song Guiwen , Zhang Di , Liu Fuli 
Published: June 2023

Abstract
Homologous to E6-associated protein Carboxyl Terminus (HECT) is one important family of E3 ubiquitin ligase superfamily involved in the ubiquitin-proteasome pathway. This gene family plays widely roles in the regulation of growth, development and signaling pathways in different organisms, as a specific key molecule in ubiquitin signal transduction. Large number of studies have focused on the identification and characterization of HECT E3 ligase in plants and animals, but studies in Ochrophyta, a phylogenetic lineage that is distantly related to terrestrial plants and animals, are very scarce. This study comprehensively analyzed the HECT E3 ligase gene family in algae Saccharina japonica with significant economic and ecological value. Eighteen HECT E3 ligase members were identified in S. japonica genome, and the physicochemical parameters, phylogenetic relationships, structural mechanisms, functional annotations, and gene expression of these E3 ligases were analyzed and characterized. Additionally, the HECT E3 genes were also identified from other five algae genomes. A total of 90 HECT E3 from the six algae species were used to elucidate the evolutionary relatedness of the HECT gene family in algae. The resulted phylogenetic tree revealed that the HECT family was mainly divided into six branches. Protein structure analysis revealed the conservation of the HECT domain in the entire gene family, showing the substrate binding and structure-function relationships. A large number of regulatory elements involved in stress, hormone regulation, light response, growth and development were identified in the promoter region of SjHECT E3 genes. Expression analysis indicated that members in different SjHECT gene subfamilies might play specific roles in the growth and heat stress response of S. japonica. Overall, the identification and analysis of the HECT E3 gene family in S. japonica laid the foundation for further study on the function and regulatory pathways of HECT in Ochrophyta species.
Right ventricular structural and functional adaptation to regular, intense exercise in the young: a 3D echocardiographic study in adolescent athletes
Authors: Ujvári Adrienn , Fábián Alexandra , Lakatos Bálint Károly , Tokodi Márton , Ladányi Zsuzsanna , Sydó Nóra , Csulak Emese , Vágó Hajnalka , Juhász Vencel , Grebur Kinga , Szűcs Andrea , Zámodics Márk , Babity Máté , Kiss Orsolya , Merkely Béla , Kovács Attila 
Published: June 2023

Abstract
Background
Data are scarce concerning the exercise-induced structural and functional adaptations in adolescent athletes, especially concerning the right ventricle (RV). We aimed to characterize the RV contraction pattern and its associations with exercise capacity in a large cohort of adolescent athletes using 3D (three dimensional) echocardiography.
Results
We enrolled 215 adolescent athletes (16±1 years, 169 males, 12±6 hours of training/week) and compared them to 38 age- and sex-matched healthy, sedentary children. 3D echocardiographic datasets were acquired. We measured the biventricular end-diastolic volume indices (EDVi) and ejection fractions (EF) along with left ventricular (LV) mass index (LVMi) using dedicated software. We also determined the relative contributions of longitudinal EF (LEF/RVEF) and radial EF (REF/RVEF) to the RVEF. Same-day cardiopulmonary exercise testing was performed to calculate VO2/kg. LV and RV volumes and LV mass were higher in athletes compared to controls (athletes vs. controls; LV EDVi: 80±13 vs 64±10, RV EDVi: 81±14 vs 68±10 ml/m2, LVMi: 84±14 vs 68±13 g/m2, p<0.01). Notably, both LV and RVEFs were significantly lower (LVEF: 57±4 vs 61±3, RVEF: 55±5 vs 60±5%, p<0.001). Interestingly, while the relative contribution of radial shortening to the global RV EF was also reduced (REF/RVEF: 0.40±0.10 vs 0.49±0.06, p<0.001), the contribution of the longitudinal contraction was significantly higher in athletes (LEF/RVEF: 0.45±0.08 vs 0.40±0.07, p<0.01). The supernormal longitudinal shortening correlated with a higher VO2/kg (r= 0.138, P = 0.044).
Conclusions
Similarly to the adult athlete’s heart, the cardiac adaptation of adolescent athletes comprises higher biventricular volumes and lower resting functional measures with supernormal RV longitudinal shortening. Our study suggests that characteristic exercise-induced structural and functional cardiac changes are already present in childhood.
Identification of two novel large deletions in FBN1 gene by next-generation sequencing and multiplex ligation-dependent probe amplification
Authors: Lu Xinxin , Wang Ren , Li Mingjie , Zhang Biao , Rao Huiying , Huang Xiaoli , Chen Xijun , Wu Yan’an 
Published: June 2023

Abstract
Background
Mutations in fibrillin 1 (FBN1) are known to be associated with Marfan syndrome (MFS), an autosomal dominant connective tissue disorder. Most FBN1 mutations are missense or nonsense mutations. Conventional molecular genetic testing of FBN1 using techniques such as Sanger sequencing may miss disease-causing mutations in promoter regions or other noncoding sequences as well as partial or complete gene deletions and duplications.
Methods
Two MFS patients referred for genetic testing were recruited and underwent next-generation sequencing, multiplex ligation-dependent probe amplification and gap PCR to identify the causative mutation.
Results
We identified two large genomic deletions in FBN1 from two MFS patients. One patient had a 0.23 Mb deletion spanning FBN1 exons 1–6 and the neighboring upstream gene CEP152. The other patient harbored a 1,416 bp deletion of FBN1 exon 66.
Conclusion
Our report expanded the number of large FBN1 deletions and highlighted the importance of screening for large deletions in FBN1 in clinical genetic testing,especially for those with classic Marfan phenotype.
Factors that influence mental health of university lecturers
Authors: Afridah Wiwik , Fitriyah Fifi Khoirul , Lestari Yanesti Nuravianda , Amin Nur , Wikurendra Edza Aria 
Published: June 2023

Abstract
Background
Mental health is one of the most important issues in education, as it directly contributes to the development and academic success of students. This study analyzes the factors that influence the mental health of university lecturers.
Methods
Descriptive correlational research using the positive mental health scale questionnaire (PMH-Scale), physical activity scale (IPAQ) and religiosity and spirituality scale (RSYS) on 92 respondents. The results were analyzed by correlation test using Chi-Square then Binary Logistic Regression test.
Results
Physical activity level, spirituality level, education level, teaching duration, and certification status were significantly related to the mental health condition of university lecturers. The most influential determinants of university lecturers mental health were spirituality level (P = .002), education level (P = .004), and certification status (P = .019).
Conclusion
The higher the level of education and accompanied by the possession of teaching certifications, the university lecturers will have increased welfare. This will significantly affect the mental health condition of university lecturers. In addition, the higher the level of spirituality of university lecturers will make the mind and heart calmer which in turn will have an impact on the stability of the mental health condition of university lecturers.
SPIRIT – Assessing clinical parameters associated with using IDegLira in patients with Type 2 Diabetes in a real-world setting in Colombia: a single arm, retrospective, chart review study
Authors: Ramírez Alex , Carrillo Diana Henao , Omeara Miguel , Oliveros Julio , Assaf José , Ordóñez Jaime , Prasad Preethy , Alzate María Alejandra 
Published: June 2023

Abstract
Aim.
Insulin degludec/liragultide (IDegLira) is a fixed-ratio co-formulation of insulin degludec (a basal insulin) and liraglutide (GLP-1RA). This study aimed to investigate clinical outcomes in people with type 2 diabetes (T2D) after initiating IDegLira treatment in a real-world setting in Colombia.
Methods.
SPIRIT is a non-interventional, single-arm, retrospective chart review study to assess clinical outcomes in people with Type 2 Diabetes (T2D). These patients were switched from basal insulin (with or without oral antidiabetics (OADs)) and initiated treatment with IDegLira a minimum of 26 ± 6 weeks before the data collection start date. We collected data from the medical records of 175 patients in 10 clinical centers across Colombia.
Results.
Compared with baseline, there was a significant reduction in HbA1c by 1.3% (95% [-CI] 1.6, -1.0) (p < 0.0001) after 26 ± 6 weeks of follow-up. The mean HbA1c at baseline was 9.1%, and the mean at the end of the study was 7.8%. In addition, IDegLira significantly reduced the absolute body weight by 1kg (95% [IC] -1.5, -0.5) (p-value < 0.0001), from a mean of 76.1kg at baseline to 75.1kg after follow-up. The mean IDegLira dose at the end of the study was 21.3 U, and no severe hypoglycemic events were observed during the follow-up period.
Conclusion.
In real-world practice, initiating IDegLira in T2D patients previously treated with basal insulin (± OAD) was associated with improved glycemic control, reduced body weight, and reduced risk of hypoglycemia.
Trial registration
ClinicalTrials.gov
#NCT05324462
First posted: April 12, 2022
Last update posted: November 9, 2022
Self-control and bed procrastination as mediators between mindfulness and sleep quality among college students during the COVID-19 pandemic
Authors: Ling Yi , Gao Bin , Jiang Bo , Zhu Suijing , Jiang Yiyao 
Published: June 2023

Abstract
Background
In recent years, sleep problems among college students have become increasingly prominent, especially in the context of the COVID-19 pandemic, and their sleep quality has deteriorated dramatically, severely affecting their physical and mental health. Numerous research studies have investigated the relationship between mindfulness and sleep quality; however, it is still unclear what psychological process underlies this relationship. In the current study, college students' bed procrastination and self-control as mediating factors in the association between mindfulness and sleep quality were investigated.
Methods
Using the convenience sampling method, 763 Chinese college students (mean age = 19.48 years, SD = 2.06) were recruited to complete self-reported questionnaires that included the Mindful Attention Awareness Scale, Self-Control Scale, Bed Procrastination Scale, and Sleep Quality Scale. All statistical analyses were performed using SPSS 23.0 software.
Results
Results showed that (a) mindfulness was positively associated with sleep quality; (b) both self-control and bed procrastination mediated the relationship between mindfulness and sleep quality, and (c) self-control and bed procrastination sequentially mediated the relationship between mindfulness and sleep quality.
Conclusion
The results demonstrate how mindfulness internally affects sleep quality and provide a set of useful recommendations for strategies and interventions that could help students improve their sleep quality during the COVID-19 pandemic, targeting both professionals and the general public.
Significantly Improved Cryopreservation of Rat Hind Limb Vascularized Composite Allografts Using the New PrC-210 Free Radical Scavenger
Authors: Fahl William E. , Nkana Zeeda H. , Gitter Maya M. , Zeng Weifeng , Dingle Aaron M. 
Published: June 2023

Abstract
Background
Vascularized composite allotransplantation (VCA) represents a promising reconstructive solution primarily conducted to improve quality of life. However, tissue damage caused by cold-ischemia (CI) storage prior to transplant represents a major factor limiting widespread application. This study investigates the addition of the novel free radical scavenger PrC-210 to UW Organ Preservation Solution (UW Solution) to supress CI-induced skeletal muscle injury in a rat hind limb amputation model.
Methods
Lewis rats received systemic perfusion of UW solution +/- PrC-210 (0mM control, 10mM, 20mM, 30mM, or 40mM), followed by bilateral transfemoral amputation. Limbs were stored in 40mL of the same perfusate at 4°C for 48 hours. Muscle punch biopsies were taken at set times over the 48 hour cold-storage period and analyzed for caspase-3,7 activity, cytochrome C levels, and qualitative histology.
Results
A single 15 second perfusion of PrC-210-containing UW Solution conferred a dose-dependent reduction of CI-induced muscle cell death over 48 hr. In the presence of PrC-210, muscle cell mitochondrial Cytochrome C release was equivalent to 0 hr controls, with profound reductions in the Caspase 3,7 apoptotic marker that correlated with limb histology. PrC-210 conferred complete prevention of ROS-induced mitochondrial lysis in vitro, as measured by Cytochrome C release.
Conclusion
The addition of 30mM PrC210 to UW Solution conffered the most consistent reduction in CI limb damage, and it warrants further investigation for clinical application in the VCA setting.
Molecular Docking MPB83 Protein on β2, β3, and α5β1 Integrins: Pathogenesis of Bovine Tuberculosis in Humans
Authors: Rahmani Salsabila , Rachmania Sheilla , Mufida Diana Chusna 
Published: June 2023

Abstract
Tuberculosis is a chronic infectious disease caused by the Mycobacterium tuberculosis complex (MTBC). Two MTBC species that causes infection in human are Mycobacterium tuberculosis (M. tuberculosis) and Mycobacterium bovis (M. bovis). Bovine tuberculosis (bTB) is a zoonotic disease with 2.55 times higher mortality rate compared to tuberculosis due to M. tuberculosis. Mycobacterium infect humans through the bonding between adhesin molecules on the bacteria surface and fibronectin receptors on the human body such as β2, β3, and α5β1 integrins. β2, β3, and α5β1 integrins are responsible for migrating bacterial activity and strengthening cell adhesion. MPB83 is a bacterial surface protein expressed more by M. bovis than by M. tuberculosis. This study aims to examine the binding of MPB83 protein to β2, β3, and α5β1 integrins as potential pathogenesis of bTB in humans by molecular docking method. Molecular docking was carried out using ClusPro to determine the interaction between the MPB83 protein and β2, β3, and α5β1 integrins based on binding energy and binding interaction model. The results showed that in silico, bonds occur between MPB83 protein and β2, β3, and α5β1 integrins which can potential for the pathogenesis of bTB in humans.
Effects of applied microbial cultures on the structure and diversity of bacterial communities associated with Phyllostachys edulis
Authors: Liu Fang , Yuan Zong-sheng , Zeng Zhi-hao , Pan Hui 
Published: June 2023

Abstract
In order to study the feasibility of microbial cultures on the artificial regulation of Moso bamboo (Phyllostachys edulis) forest. This study used the microorganisms isolated in the previous research to prepare microbial cultures, and studied the degree of its influence on the bacterial community of Moso bamboo tissue and soil. We collected 36 samples of bamboo whip, whip root, rhizosphere soil, and non-rhizosphere soil of P. edulis before and after the application of microbial cultures. Genomic DNA was extracted and Illumina high-throughput sequencing technology was used to analyze the composition and changes of bacterial communities before and after the application of microbial cultures. Twenty-nine phyla, 96 classes, 229 orders, 444 families, and 974 genera of bacteria were identified from all samples. The dominant phyla of the sample bacteria were Proteobacteria, Acidobacteria, Actinobacteria, Chloroflexi, Firmicutes, Myxomycetes, and Bacteroidetes. Treatment with microbial cultures did not alter the bacterial community in the rhizomes, rhizome roots, rhizosphere, and non-rhizosphere soil of P. edulis. However, the bacterial diversity indices of the rhizomes and rhizome roots of P. edulis increased with time after treatment. The relative abundance of Firmicutes was most affected by the application of microbial cultures, and the rhizosphere soil samples were least affected by the application. Venn diagram and principal coordinate analyses confirmed that the composition of the bacterial community was affected by microbial cultures, but with time, the effect became smaller. Our findings provide a theoretical basis for studies on relationships between the growth of P. edulis and the microbiome, and further provide experimental evidence for the transformation of P. edulis through microbial regulation.
Association between preoperative frailty and myocardial injury after noncardiac surgery in geriatric patients: Study protocol for a prospective, multicentre, real-world observational, cohort trial
Authors: Sun Yongtao , Guo Na , Zhang Min , Liu Mengjie , Gao Zhongquan , Sun Tao , Gao Xiaojun , Xu Lingling , Zhang Haixia , Wei Chuansong , Liu Peng , Liu Yang , Zhang Xiaoning , Guo Yongle , Chen Lina , Zhou Zheng , Su Zhenqiang , Hu Yanmei , Shi Xin , Huang Linlin , Wang Yuelan 
Published: June 2023

Abstract
Introduction:
Frailty has become a worldwide health burden that has a large influence on public health and clinical practice. The incidence of frailty is anticipated to increase as the aging population increases. Myocardial injury after noncardiac surgery (MINS) is associated with short-term and long-term mortality. The incidence of MINS in geriatric frail patients is unknown.
Methods and analysis:
This prospective, multicentre, real-world observational cohort study will be conducted at 18 designated centres in China from January 2023 to December 2024, with an anticipated sample size of 856 patients aged 65 and older who are scheduled to undergo noncardiac surgery. The primary outcome measure will be the incidence of MINS. MINS is defined as a fourth-generation plasma cardiac troponin T (cTnT) concentration ≥ 0.03 ng/mL at least once within 30 days after surgery, with or without symptoms of myocardial ischaemia. All data will be collected using research electronic data acquisition.
Discussion
This study will explore the incidence of MINS in frail patients. The characteristics, predictive factors and 30-day outcomes of MINS in frail patients will be further investigated to lay the foundation for finding clinical interventions.
Clinical trial registration:
https://beta.clinicaltrials.gov/study/NCT05635877 , NCT05635877
Physiological and molecular responses of a resistant and susceptible wheat cultivar to the fungal wheat pathogen Zymoseptoria tritici
Authors: Gohari Amir Mirzadi , Noei Fateme Ghiasi , Ebrahimi Amin , Ghanbari Mohammad Amin , Didaran Fardad , Farzaneh Mohsen , Mehrabi Rahim 
Published: June 2023

Abstract
Background
Zymoseptoria tritici is the causal agent of Septoria tritici blotch, one of the most economically destructive wheat foliar diseases. Here, we investigate the physiological and molecular changes triggered in two contrastingly responding wheat cultivars upon infection by Z. tritici. To accomplish this, we combined a qPCR with a targeted quantitative HPLC technique to evaluate the expression profiles of 13 defense-related genes and to profile the polyphenolic compounds induced differentially in two applied interactions.
Results
Our finding indicated that five out of 13 genes were strongly up-regulated in the resistant cultivar compared with that of the susceptible one at eight days post-inoculation (dpi), corresponding to the transition phase present in the infection process of Z. tritici. Additionally, our targeted HPLC analysis demonstrated that the traced phenolic compounds were highly elevated in the susceptible cultivar infected by Z. tritici compared with that of the resistant cultivar. Finally, our quantitative histopathological study showed that H2O2 accumulated in the resistant cultivar to a higher degree compared to the susceptible one at the biotrophic and switching phase.
Conclusion
To sum up, we conclude that Z. tritici mediates physiological and molecular alternations upon infection of wheat plants. Furthermore, the transition phase is likely a turning point in mounting incompatibility/compatibility in Z. tritici-wheat relationship, requiring further experiments to elucidate this claim in further detail.
USP36 mediates Doxorubicin-induced cardiomyopathy through inhibiting ubiquitination and degradation of PARP1
Authors: Wang Dongchen , Jiang Zihao , Kan Junyan , Jiang Xiaomin , Pan Chang , You Shijie , Chang Ruirui , Zhang Juan , Yang Hongfeng , Zhu Linlin , Gu Yue 
Published: June 2023

Abstract
Doxorubicin (Dox) is a powerful antineoplastic agent, but its usage is limited by the severe cardiotoxicity referred to as Dox-induced cardiomyopathy (DIC). However, the molecular mechanism underlying this cardiotoxicity is yet to be fully elucidated. Here, our current study sought to determine the role of ubiquitin-specific protease 36 (USP36), a nucleolar deubiquitinating enzyme (DUB), in the progress of DIC and its mechanism. We identified an increased expression of USP36 both in neonatal rat cardiomyocytes and H9C2 cells exposed to Dox, and USP36 silencing significantly ameliorated Dox-induced oxidative stress injury and apoptosis in vitro. Mechanistically, USP36 upregulation was observed to positively correlate with PARP1 expression, and its knockdown resulted in reduction of PARP1 levels. Further investigation showed that USP36 could bind to and mediate the deubiquitination of PARP1 and increase its protein stability in cardiomyocytes upon Dox exposure. Moreover, overexpression of wild-type (WT) USP36 plasmid, but not its catalytic-inactive mutant (C131A), stabilizes PARP1 in HEK293T cells. Herein, we also established DIC model in mice and observed a significant upregulation of USP36 in the heart. Cardiac knockdown of USP36 in mice by a type 9 recombinant adeno-associated virus (rAAV9)-shUSP36 significantly preserved cardiac function after Dox treatment and protected against Dox-induced in terms of structural changes within the myocardium. Collectively, these findings indicate that Dox promotes DIC progression by activating USP36-mediated PARP1 deubiquitination. This novel USP36/PARP1 axis may play an important regulatory mechanism in the pathogenesis of DIC.