bluuebunny/crossref_metadata_2025_split huggingface dataset:
History of advances in genetic engineering of viruses before COVID-19 pandemic
Authors: Teppone Mikhail 
Published: March 2023

Background:
On December 31, 2019, the World Health Organization’s China Country Office was alerted to cases of pneumonia of unknown cause detected in Wuhan City, Hubei Province of China.

Methods:
Due to the fact that to date, the question of the origin of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has not been resolved yet, the author analyzed the main advances in the development of genetic engineering of viruses that took place before the onset of the COVID-19 pandemic.

Results:
The first artificial genetically modified viruses could appear in nature in the mid-1950s. The technique of nucleic acid hybridization was developed by the end-1960s. In the late 1970s, a method called the “reverse genetics” emerged to synthesize ribonucleic acid and deoxyribonucleic acid molecules. In the early 1980-s, it became possible to combine the genes of different viruses and insert the genes of one virus into the genome of another virus. Since that time, the production of vector vaccines began. At present, by modern technologies one can assemble any virus based on the nucleotide sequence available in the virus database or designed by a computer as a virtual model.

Conclusion:
Scientists around the world are invited to answer the call of Neil Harrison and Jeffrey Sachs of Columbia University, for a thorough and independent investigation into the origin of SARS-CoV-2. Only a full understanding of the origin of the new virus can minimize the likelihood of a similar pandemic in the future.
“This is Also Familiar to Me...”: Explicit and Hidden Meanings of Table Music in the Second Act Finale of “Don Giovanni” by W. A. Mozart
Authors: Денисов А.В. 
Published: March 2023

В статье исследуются три цитаты в финале второго акта «Дон Жуана» В. А. Моцарта. Они редко привлекали внимание исследователей. Вместе с тем интерпретация застольной сцены у Моцарта и в целом, и в отдельных деталях оказывается весьма необычной. Попурри на основе заимствованной музыки в опере XVIII в. — явление экстраординарное. Показано, что все три цитаты представляют смысловые комментарии не только к тому, что происходит в данный момент на сцене, но и к сценическим положениям, свершившимся в опере ранее, а также к тому, что в ней вскоре произойдет. Выдвигается предположение, что данную сцену финала можно рассматривать как функциональный аналог «спектакля в спектакле», представленного в некоторых операх XVIII в. (например, в «Аксуре» А. Сальери). Кроме этого застольная сцена финала второго акта имеет композиционную параллель со сценой финала первого акта, представляющей quodlibet из трех танцев: в обоих случаях празднично-пиршественные образы ликования жизни и любви внезапно прерываются, напоминая о грядущем возмездии главному герою. Столь сложная организация с различными смысловыми ссылками и параллелями, уникальная для жанра оперы-buffa, вновь свидетельствует о многоплановости его трактовки у Моцарта.
The article examines three quotes in the finale of the second act of “Don Giovanni” by W. A. Mozart. This scene rarely attracted the attention of researchers. At the same time, Mozart's interpretation of the table scene, both in general and in individual details, turns out to be very unusual. A potpourri based on borrowed music in XVIIIth-century opera is an extraordinary phenomenon. It is shown that all three quotations represent semantic comments not only on what is currently happening on the stage, but also on the stage situations that took place in the opera earlier, as well as on what will happen in it soon. It is suggested that this scene of the finale is a functional analogue of the “performance in a performance” presented in some operas of the XVIIIth century (for example, in “Axur” by A. Salieri). In addition, the table scene of the second act finale is a compositional parallel to the scene of the first act finale, representing a quodlibet of three dances — in both cases, the festive and feasting images of life and love jubilation are suddenly interrupted, reminding the soon coming retribution to the main hero. Such a complex organization with various semantic references and parallels, unique for the opera buffa genre, again testifies to the versatility in its interpretation by Mozart.
Arkhangelsk Materials in the Funds of the Klyment Kvitka Folk Music Research Center (General Characteristics)
Authors: Битерякова Е.В. 
Published: March 2023

Статья представляет обзор звуковых и рукописных документов архангельской коллекции из фондов НЦНМ имени К. В. Квитки. История собирания музыкального фольклора на территориях Русского Севера, не являвшихся приоритетными для фольклористов Московской консерватории, вписана в контекст общей истории отечественной этномузыкологии ХХ века. Направления собирательской работы отражают ключевые тенденции соответствующих периодов в советской музыкально-фольклористической науке. В 1930-е годы остро стоял вопрос обновления народных традиций в иных социально-идеологических условиях. В 1950-е годы поиск новых форм оставался по-прежнему важной собирательской задачей, однако записи частушечных и других «современных» и «осовремененных» текстов не препятствовали интересу исследователей к архаическим жанрам в их исконном виде. В этом смысле примечательно соседство в экспедиционных материалах частушек и былин, свадебных приговоров и сказов о Ворошилове, Ленине и Сталине. Записи 1960-х годов, наиболее многочисленные и разнообразные, демонстрируют, среди прочего, не свойственный музыковедам-фольклористам интерес к немузыкальным жанрам: заговорам, приговорам, сказкам, стихам, загадкам. Статья завершается выводом о необходимости внимательного изучения и введения документов архангельской коллекции НЦНМ в широкий научный оборот.
The article is devoted to the characteristics of the Arkhangelsk collection from the funds of the Klyment Kvitka Folk Music Research Center of the Moscow State Tchaikovsky Conservatory. The history of collecting musical folklore in the territories of the Russian North, which were not a priority for the folklorists of the Moscow Conservatory, is inscribed in the context of the history of Russian ethnomusicology of the 20th century. The character of the fieldwork show key trends of particular periods in Soviet musical ethnography. For the period of 1930s it was the problem on updating folk traditions according to the new socio-ideological conditions. In the 1950s, the search for new forms was still an important task, but recording of chastushkas (couplets) and other “modern” and “modernized” texts did not conflict with the interest of the folklorists in archaic genres in their original form. In this sense, it is remarkable to note how chastushkas, epics, and wedding whisperings go in the expedition materials along with tales about Soviet leaders and heroes of the October revolution of 1917 and the subsequent civil war. The documents of the 1960s, the most numerous and diverse, demonstrate researchers’ interest in non-musical genres (which is not peculiar for musicologists-folklorists): spells, whisperings, fairy tales, poems, riddles. The author makes a conclusion about relevance of introducing the documents of the Arkhangelsk collection of the Klyment Kvitka Folk Music Research Center into a wide scientific circulation.
Using detrending methods for intelligent processing of construction process monitoring data
Authors: Kagan Pavel , Parshin Dmitriy 
Published: March 2023

Data processing of monitoring systems of various processes at the stage of construction and operation of buildings requires the development of special tools that belong to the field of artificial intelligence. The trend removal method is one of the ways to preprocess data collected from various sensors and IoT devices that monitor the state of buildings, structures and soil masses during construction and operation. This article analyzes how different approaches to detrending time series affect the performance and accuracy of algorithms for CI computational intelligence models. The analysis compares three approaches: linear detrending, non-linear detrending, and first-order differentiation.
Five representative methods are used as CI models: DENFIS dynamic evolving fuzzy neural network, GP Gaussian process, MLP multilayer perceptron, OP-ELM optimally trimmed extremal learning machine, and SVM support vector machine. There are three main conclusions from the experiments performed on the four datasets: 1) detrending does not improve overall performance, 2) the empirical mode decomposition method provides better performance than linear detrending, and 3) first-order differentiation in some cases can be effective and in some cases counterproductive for series with common patterns.
Identification of potential human chymase inhibitors using molecular docking and molecular dynamics simulation
Authors: Rozindar Asiya , DBM Virupakshaiah , Meti Bharati , Vasant Kumbha Bajarang , Oli Ajay Kumar 
Published: March 2023

Chymase is a hydrolase class of enzymes that involves hydrolysis of peptide bonds. It is abundant in secretory granules of mast cells. Mast cell chymase is involved in the synthesis of angiotensin-II from its precursor protein. In addition, chymase is involved in converting TGF-β and matrix metalloproteinase to their active form. Chymase involved in heart failure has been proven, and its inhibition may reduce the progression. Hence, to identify the potential inhibitors against the chymase, the present study employed structure-based virtual screening, molecular docking, and molecular dynamics simulation to identify potential chymase inhibitors. Initially, compounds were selected based on their physicochemical and pharmacokinetic properties. Further, the binding affinities using molecular docking and interaction analyses were performed to find potential chymase inhibitors. The study identified chymase inhibitor ZINC000008382327, bearing significant binding affinity, specificity, and efficacy towards the chymase. Next, the stability and binding mode of chymase with ZINC000008382327 were assessed using molecular dynamics simulations. The simulation analysis using root mean square deviations and fluctuations revealed that inhibitor ZINC000008382327 affected the structure and dynamics of the chymase protein. It was also shown that the chymase forms a stable complex with ZINC000008382327 during the simulation. Thus, the present computational study put forward that the identified compound can be further exploited as a potential chemical scaffold to design and develop new human chymase inhibitors.
Using food residues (potato peels) as an alternative to potato dextrose agar in the growth of edible food fungi
Authors: Almjalawi Batool Shakir Abed , Chechan Rukaibaa Ali , AL-Hadedee Lamees Thamer 
Published: March 2023

Due to the great importance of edible fungi, their rapid life cycle, and the possibility of their production throughout the year, their cultivation has become a vital resource that many countries depend on in their pursuit of economic growth and food security. The present study aimed to devise a local nutrient media for primary mycelium growth of nine species of fungi belonging to Pleurotus eryngii , Pleurotus floridanus , Pleurotus ostreatus (white strain) , Agaricus heterocystis , Agaricus bispous (brown strain ), Agaricus bispous (white strain), Lentinula edodesstrain No. 1, Lentinula edodes strain No. 2, Flammulina velutipe (yellow strain) using culture mycelium inoculation for reproduction and production of edible fungi. Different natural materials used for this purpose involved potato peels in a concentration ranging from 5 to 20 g/ L and compared with the commercial medium Potato Dextrose Agar at a rate of 39 g/Land according to the manufacturer's instructions. The study also focused on the effect of physical factors such as temperature and pH on the growth rate of fungal mycelium. The results showed that the media prepared from potato peels at 5 g/L and 10 g/L concentrations achieved the best growth rate for the studied fungi compared to Peptone Dextrose Agar (PDA). The increase in the growth rate of fungi on media prepared from potato peels was attributed to the effect of nutrients for the prepared medium because it contained effective and necessary compounds for the growth in addition to their ease of absorption and consumption to be ready for food for agricultural fungi. The effect on the growth and production of edible fungi or any other type of fungi has not previously been studied and compared to the commercial medium PDA.
Enhacment of biomass, carbohydrates, lipids, and proteins content using co-culture of Glagah consortium and Lipomyces starkeyi
Authors: Sri Winasti Ni Made , Yulyanita Dita Aulia , Naser Ahmad Saifun , Suyono Eko Agus 
Published: March 2023

Microorganisms have a high potential as biofuel sources. Co-culture of microalgae and yeasts can result in high lipid production as a modification treatment. The goal of this study was to see how the co-culture of the Glagah consortium (diversity of associated microalgae and bacteria from Glagah Lagoon, Yogyakarta) and Lipomyces starkeyi affected the production of biomass, lipids, proteins, and carbohydrates. The culture was performed under airtight conditions on a shaker at 127 rpm, with a light intensity of 27.75 mol/m2/s and a temperature of 30°C. The culture was subjected to a dark: light (6:18) treatment. Biomass was measured by dry weight, lipids by the Bligh and Dyer method, proteins by the Bradford method and carbohydrates by the phenol-sulfuric acid method. On day 3, L. starkey culture produced the most biomass, yielding 2.21 g/L with a productivity of 0.49 g/L/day. On day 4, the highest lipids produced from co-culture treatment yielded 1.03 g/g with a productivity of 0.21 g/L/day. The highest protein yield was obtained from L. starkeyi culture treatment on day 4, yielding 0.60 g/g with a productivity of 0.12 g/L/day. On day 6, co-culture produced the total carbohydrates, yielding 4.78 g/g with a productivity of 0.68 g/L/day. The co-culture treatment produced the highest lipids and carbohydrates production (1.03 g/g and 4.78 g/g) and productivity (0.21 g/L/day and 0.68 g/L/day), while L. starkeyi culture produced the highest total biomass and protein production (2.21 g/L and 0.6 g/g) and productivity (0.49 g\L\day and 0.12 g/L/day). In microalgae culture, CO2 generally given directly through the aeration process. In this study, the source of CO2 was yeast, whereas yeast also obtained O2 from microalgae in the consortium for their metabolic process. This mutualism symbiosis will help in providing benefits in reducing the costs for the cultivation process, especially in optimizing the production of biomass an lipids.
GASTOS TRIBUTÁRIOS COM O ESPORTE: LEI DE INCENTIVO FISCAL (LEI Nº 11.438/2006)
Authors: Matias Wagner Barbosa , Elicker Eliane , Pereira Cláudia Catarino , Dos Santos Mariângela Ribeiro , Mascarenhas Fernando 
Published: March 2023

RESUMO:
A presente investigação pretende refletir sobre os gastos tributários do governo federal, especificamente acerca da aplicação dos recursos da Lei de Incentivo ao Esporte (lei nº 11.438/2006). A investigação se apoiou nos documentos oficiais publicados pelo governo federal e se caracteriza como uma pesquisa exploratória, de cunho qualitativo e quantitativo. Os dados são do período inicial de vigência da referida Lei, (agosto de 2007 a dezembro de 2015) e o segundo período (2016 a 2018). A análise e a discussão dos resultados se concentram no debate sobre as categorias fundo público, gastos tributários e o financiamento do esporte. Observou-se que há uma baixa captação dos recursos e uma concentração na sua destinação por região, manifestação esportiva, modalidade, proponentes e na realização de eventos.
Tingkat Kepuasan Pasien BPJS dan Pasien Umum
Authors: Yanuarti Riska , Oktavidiati Eva , Febriawati Henni , Oktarianita Oktarianita 
Published: March 2023

This study aims to determine the difference in satisfaction of BPJS patients and general patients at the Betungan Health Center, Bengkulu City. This research is a descriptive study with a cross-sectional design. The results of the BPJS patient satisfaction study were 20 respondents (50%) were satisfied. General patient target as many as 21 respondents (52.5%) was satisfied. The results of statistical tests obtained p-value satisfaction of BPJS patients and general patients based on tangible dimensions <0.05 (p=0.025), reliability dimension (p=0.001), responsiveness dimension (p=0.001), assurance dimension (p=0.001) and empathy dimension (p=0.001). In conclusion, there are differences in the satisfaction of BPJS patients and general patients at the Betungan Health Center, Bengkulu City.

Keywords: BPJS, General Patients
Peran Kader Posyandu terhadap Tingkat Kunjungan Masyarakat
Authors: Haritani Hartini , Hariadi Puspawan , Oktresia Ewisa , Sovia Fitriwati , Gemantari Baiq Maylinda 
Published: March 2023

This study aims to describe the role of posyandu cadres on the level of visits by the users. The method used in this study is descriptive qualitative with a program evaluation approach. The results obtained from this study are that the number of visits by pregnant women, toddlers, and the elderly has increased. However, a strategy is needed to increase the interest of young women to utilize posyandu so that they can increase the number of visits by adolescents. In conclusion, the role of Posyandu cadres dramatically contributes to the health needs of small communities, especially in villages.
Keywords: Cadres, Posyandu, Healthy
Menilai Perkembangan Motorik Kasar Anak Melalui Permainan Lompat Tali
Authors: Ferasinta Ferasinta , Padila Padila , Anggita Rahma 
Published: March 2023

This study aims to determine the effect of rope jumping games on gross motor skills in children. This study uses a Quasy Experiment research design with a One Group Pre-Post Design approach. The results of this study show the effect of jumping rope on gross motor development in children with pre-test and post-test data of jumping rope with p-value = 0.001. In conclusion, rope jumping games affect gross motor development in children because rope jumping games have jumping movements in a balanced and coordinated manner.
Keywords: Jumping Rope, Jumping, Gross Motor
The Influence of Nursing Skills Learning Media Through Video on the Achievement of Student Competencies in the Installation of Infusions
Authors: PAY Jenny Ramadona , Sundari Sri 
Published: March 2023

This study aims to analyze how the influence of video learning media on student competency achievement. The method used in this study was carried out by taking data from PubMed, ProQuest, Google Scholar databases, which were found in 2015-2021. The results showed that some of the learning media used by students were video. In conclusion, video as a learning medium is very influential in increasing knowledge, helping to get information about assignments, knowing the development of information, and can be used as material to deepen the material.

Keywords: Students, Learning Media, Video
Chronic Management during Pandemic COVID-19 from the Perspective Primary Health Care Practitioners
Authors: Alhalawi Zikri , Sutantri Sutantri 
Published: March 2023

This study aims to determine how chronic care management for the covid-19 pandemic is managed from the point of view of public health by health practitioners. The method of this research is to use a literature review using the Proquest, Pubmed, Science Direct, and Ebsco databases. The results showed that 718 articles and seven met the criteria for inclusion in the author's review. In conclusion, chronic care management during the Covid-19 pandemic from the perspective of primary health practitioners is very influential in how people with chronic diseases can survive and be helped by improving the quality of their care.

Keywords: COVID-19, Primary Health Services and Nurses, Management, Chronic Care
The significance of hindbrain herniation reversal following prenatal repair of neural tube defects
Authors: Brock Clifton O. , Bergh Eric P. , Fishel Bartal Michal , Johnson Anthony , Hernandez-Andrade Edgar A. , Garnett Jeannine , Tsao KuoJen , Austin Mary T. , Fletcher Stephen A. , Johnston Jennifer H. , Hughes Katrina S. , Patel Rajan , Papanna Ramesha 
Published: March 2023

OBJECTIVE
The aim of this study was to determine whether reversal of hindbrain herniation (HBH) on MRI following prenatal repair of neural tube defects (NTDs) is associated with reduced rates of ventriculoperitoneal (VP) shunt placement or endoscopic third ventriculostomy (ETV) within the 1st year of life.

METHODS
This is a secondary analysis of prospectively collected data from all patients who had prenatal open repair of a fetal NTD at a single tertiary care center between 2012 and 2020. Patients were offered surgery according to inclusion criteria from the Management of Myelomeningocele Study (MOMS). Patients were excluded if they were lost to follow-up, did not undergo postnatal MRI, or underwent postnatal MRI without a report assessing hindbrain status. Patients with HBH reversal were compared with those without HBH reversal. The primary outcome assessed was surgical CSF diversion (i.e., VP shunt or ETV) within the first 12 months of life. Secondary outcomes included CSF leakage, repair dehiscence, CSF diversion prior to discharge from the neonatal intensive care unit (NICU), and composite neonatal morbidity. Demographic, prenatal sonographic, and operative characteristics as well as outcomes were assessed using standard univariate statistical methods. Multivariate logistic regression models were fit to assess for independent contributions to the primary and secondary outcomes.

RESULTS
Following exclusions, 78 patients were available for analysis. Of these patients, 38 (48.7%) had HBH reversal and 40 (51.3%) had persistent HBH on postnatal MRI. Baseline demographic and preoperative ultrasound characteristics were similar between groups. The primary outcome of CSF diversion within the 1st year of life was similar between the two groups (42.1% vs 57.5%, p = 0.17). All secondary outcomes were also similar between groups. Patients who had occurrence of the primary outcome had greater presurgical lateral ventricle width than those who did not (16.1 vs 12.1 mm, p = 0.02) when HBH was reversed, but not when HBH was persistent (12.5 vs 10.7 mm, p = 0.49). In multivariate analysis, presurgical lateral ventricle width was associated with increased rates of CSF diversion before 12 months of life (adjusted OR 1.18, 95% CI 1.03–1.35) and CSF diversion prior to NICU discharge (adjusted OR 1.18, 95% CI 1.02–1.37).

CONCLUSIONS
HBH reversal was not associated with decreased rates of CSF diversion in this cohort. Predictive accuracy of the anticipated benefits of prenatal NTD repair may not be augmented by the observation of HBH reversal on MRI.