bluuebunny/crossref_metadata_2025_split huggingface dataset:
Potential and prospects of biomass as a source of renewable energy in Pakistan
Authors: Ali Asad , Ayub Khurram Shahzad , Tahseen Sadiq Muhammad , Tanveer Muhammad , Naseer Hamza , Nadeem Zoha , Aamir Hafiz Muhammad 
Published: July 2023

In an agricultural country like Pakistan, producing affordable and clean energy can be a challenging task. However, Pakistan has the potential to utilize various biomass feedstocks to generate renewable energy and tackle climate change while promoting sustainable development. Wheat, rice, sugarcane, and corn are the four main crops that yield a significant amount of residue, totaling 112.1 million tons per year. These residues have the potential to produce 3,050 kWh/ton of energy, which can meet 14% of the energy demand in Pakistan, equivalent to 9.85TW, starting in 2022. Gasification technology is a versatile option that efficiently converts biomass into energy while reducing negative environmental impacts. The current research explores the feasibility of generating clean energy from crop residues with low emissions, addressing the country’s energy needs, and supporting policymakers in promoting the use of biomass for energy production. According to this study, rice husk, corn cobs, wheat straw, and sugar bagasse all produce hydrogen at rates of 6.9 wt.%, 6.4 wt.%, 5.69 wt.%, and 5.35 wt.%, respectively. Therefore, our study demonstrates that corn cobs have a significant potential for energy production.
The potential impact of hereditary hemochromatosis on the heart considering the disease stage and patient age—the role of echocardiography
Authors: Świątczak Michał , Rozwadowska Katarzyna , Sikorska Katarzyna , Młodziński Krzysztof , Świątczak Agata , Raczak Grzegorz , Daniłowicz-Szymanowicz Ludmiła 
Published: July 2023

BackgroundHereditary hemochromatosis (HH) is a genetic disease that leads to increased iron accumulation in several organs. Cardiomyocytes are highly susceptible to this damage owing to their high iron uptake, and cardiovascular complications account for 1/3 of the deaths in the natural course of HH. Additionally, excess iron intake and associated oxidative stress may accelerate the aging of the cardiovascular system, regardless of the age of patients with HH. We aimed to investigate the role of standard and speckle-tracking echocardiography (STE) in revealing heart differences in patients with HH considering the disease stage and the patient age.MethodologyConsecutive patients with HH (n = 58) without heart pathologies (except hypertension) and 29 age- and sex-matched healthy individuals underwent echocardiography. Patients were compared according to the time since HH diagnosis (the recently diagnosed HH group [31 patients] with diagnosed HH for less than 6 months and had no more than one venesection; the medium group [11 patients] with diagnosed HH between 6 and 24 months; and the long-lasting group [16 patients] with diagnosed HH for more than 2 years) and the quartile contribution of their age.ResultsStandard echocardiography revealed differences in diastolic parameters between patients with HH and controls, which were the most prominent between healthy and long-lasting HH patients. Regarding systolic function, left ventricular ejection fraction was lower in HH patients, with the most evident differences between the healthy and recently diagnosed HH patients. STE revealed additional differences in systolic parameters, with LV rotation the worst in recently diagnosed patients and its increase in patients with medium and long-lasting HH. Significantly worse peak systolic longitudinal strain values were observed in all patients with HH. Analyses of the results according to the age quartiles of patients with HH revealed that some changes ocurred earlier than expected according to age.ConclusionsEchocardiography can reveal possible heart damage in HH patients at different stages of the disease and highlight potential features of accelerated myocardial aging in these patients.
Short-term mechanical support with the Impella 5.x for mitral valve surgery in advanced heart failure—protected cardiac surgery
Authors: Osswald Anja , Shehada Sharaf-Eldin , Zubarevich Alina , Kamler Markus , Thielmann Matthias , Sommer Wiebke , Weymann Alexander , Ruhparwar Arjang , El Gabry Mohamed , Schmack Bastian 
Published: July 2023

IntroductionSurgical treatment of patients with mitral valve regurgitation and advanced heart failure remains challenging. In order to avoid peri-operative low cardiac output, Impella 5.0 or 5.5 (5.x), implanted electively in a one-stage procedure, may serve as a peri-operative short-term mechanical circulatory support system (st-MCS) in patients undergoing mitral valve surgery.MethodsBetween July 2017 and April 2022, 11 consecutive patients underwent high-risk mitral valve surgery for mitral regurgitation supported with an Impella 5.x system (Abiomed, Inc. Danvers, MA). All patients were discussed in the heart team and were either not eligible for transcatheter edge-to-edge repair (TEER) or surgery was considered favorable. In all cases, the indication for Impella 5.x implantation was made during the preoperative planning phase.ResultsThe mean age at the time of surgery was 61.6 ± 7.7 years. All patients presented with mitral regurgitation due to either ischemic (n = 5) or dilatative (n = 6) cardiomyopathy with a mean ejection fraction of 21 ± 4% (EuroScore II 6.1 ± 2.5). Uneventful mitral valve repair (n = 8) or replacement (n = 3) was performed via median sternotomy (n = 8) or right lateral mini thoracotomy (n = 3). In six patients, concomitant procedures, either tricuspid valve repair, aortic valve replacement or CABG were necessary. The mean duration on Impella support was 8 ± 5 days. All, but one patient, were successfully weaned from st-MCS, with no Impella-related complications. 30-day survival was 90.9%.ConclusionProtected cardiac surgery with st-MCS using the Impella 5.x is safe and feasible when applied in high-risk mitral valve surgery without st-MCS-related complications, resulting in excellent outcomes. This strategy might offer an alternative and comprehensive approach for the treatment of patients with mitral regurgitation in advanced heart failure, deemed ineligible for TEER or with need of concomitant surgery.
Phthalate esters in municipal sewage treatment plants: occurrence level, removal rate and optimum combination technology
Authors: Zheng Xiqiang , Lu Guanghua , Liu Jianchao , Jiang Runren 
Published: July 2023

The concentrations, and removal rates of five phthalate esters (PAEs) (dimethyl phthalate (DMP), diethyl phthalate (DEP), dibutyl phthalate (DBP), diisobutyl phthalate (DiBP) and di (2-ethylhexyl) phthalate (DEHP)) in the five representative sewage treatment plants of Chaohu catchment were investigated by season in this study. Our results showed that five PAEs were ubiquitous contaminants in the five sewage treatment plants. Thereinto, the contents of DiBP were significantly higher than that of the other four kinds of PAEs, and the total concentrations of PAEs in the influents in summer (17.51–33.93 μg L−1) were significantly higher than those in winter (10.14–24.08 μg L−1). Different PAEs showed different removal efficiencies in sewage treatment plants, and the orders of individual removal rates was as follows: DMP &gt; DEP &gt; DBP &gt; DiBP &gt; DEHP. The total concentration of PAEs in the effluent was relatively low in Chaohu catchment. The biochemical treatment section of the sewage treatment plant contributed the most to the removal of total PAEs, while the pretreatment and advanced treatment sections contributed less. In addition, environmental temperature had an obvious effect on the biochemical treatment process. Appropriate combined treatment processes could efficiently improve the removal effect of PAEs in sewage treatment plants.
Interaction force mechanism for the improvement of reclaimed soil aggregate stability in abandoned homestead by different organic-inorganic soil conditioners
Authors: Liu Zhe , Zhang Yang , Sun Yingying , Li Xuxiang , Wang Na , Wang Xue , Meng Tingting 
Published: July 2023

Reasonable application of organic-inorganic soil conditioners can effectively improve the structure and fertility of reclaimed soil in abandoned homestead. Aggregate stability is an important indicator to evaluate soil structure and fertility, and is largely influenced by soil internal forces (van der Waals attractive force, electrostatic repulsive force, hydration repulsive force) and particle surface properties. However, there are few studies on the influence of different soil conditioners on the reclaimed soil internal forces and its relationship with the aggregate stability. Therefore, we selected six different treatments of organic fertilizer (TO), fly ash (TF), maturing agent (TM), maturing agent + organic fertilizer (TMO), fly ash + organic fertilizer (TFO) and control (CK) to conduct a 5-year field experiment to study the effects of reclaimed soil particle interaction forces and surface characteristics on aggregate stability under the treatment of different soil conditioners. The results showed that with the application of soil conditioners, the soil organic matter (SOM), specific surface area (SSA), surface charge (σ0), cation exchange capacity (CEC), aggregate mean weight diameter (MWD) and Hamaker constant increased gradually, while the pH value decreased slightly. In particular, the MWD under the treatments of TFO and TMO increased by 150.3% and 65.6% respectively compared with that under the CK treatment. With the increasing application of soil conditioners, the electrostatic repulsive force and van der Waals attractive force between reclaimed soil particles increased constantly, but the net resultant force between particles decreased and the net attractive force increased continuously, thus improving the aggregate stability. Therefore, there is a significant negative correlation between the net resultant force among reclaimed soil particles and MWD and CEC. In addition, 10−2 mol L-1 is the critical concentration that affects the reclaimed soil internal force, and the organic-inorganic treatments of TFO and TMO can improve the net resultant force better. In a word, the particle interaction forces are important factors affecting the reclaimed soil structural stability, and this study provides a scientific reference for the rational selection of soil conditioners and its interaction force mechanism in the reclaimed soil improvement.
Identification of immune infiltration and cuproptosis-related molecular clusters in tuberculosis
Authors: Li Sijun , Long Qian , Nong Lanwei , Zheng Yanqing , Meng Xiayan , Zhu Qingdong 
Published: July 2023

BackgroundTuberculosis (TB) is an infectious disease caused by Mycobacterium tuberculosis (Mtb) infection. Cuproptosis is a novel cell death mechanism correlated with various diseases. This study sought to elucidate the role of cuproptosis-related genes (CRGs) in TB.MethodsBased on the GSE83456 dataset, we analyzed the expression profiles of CRGs and immune cell infiltration in TB. Based on CRGs, the molecular clusters and related immune cell infiltration were explored using 92 TB samples. The Weighted Gene Co-expression Network Analysis (WGCNA) algorithm was utilized to identify the co-expression modules and cluster-specific differentially expressed genes. Subsequently, the optimal machine learning model was determined by comparing the performance of the random forest (RF), support vector machine (SVM), generalized linear model (GLM), and eXtreme Gradient Boosting (XGB). The predictive performance of the machine learning model was assessed by generating calibration curves and decision curve analysis and validated in an external dataset.Results11 CRGs were identified as differentially expressed cuproptosis genes. Significant differences in immune cells were observed in TB patients. Two cuproptosis-related molecular clusters expressed genes were identified. Distinct clusters were identified based on the differential expression of CRGs and immune cells. Besides, significant differences in biological functions and pathway activities were observed between the two clusters. A nomogram was generated to facilitate clinical implementation. Next, calibration curves were generated, and decision curve analysis was conducted to validate the accuracy of our model in predicting TB subtypes. XGB machine learning model yielded the best performance in distinguishing TB patients with different clusters. The top five genes from the XGB model were selected as predictor genes. The XGB model exhibited satisfactory performance during validation in an external dataset. Further analysis revealed that these five model-related genes were significantly associated with latent and active TB.ConclusionOur study provided hitherto undocumented evidence of the relationship between cuproptosis and TB and established an optimal machine learning model to evaluate the TB subtypes and latent and active TB patients.
A decade of microbial community dynamics on sinking particles during high carbon export events in the eastern Fram Strait
Authors: Cardozo-Mino Magda G. , Salter Ian , Nöthig Eva-Maria , Metfies Katja , Ramondenc Simon , Wekerle Claudia , Krumpen Thomas , Boetius Antje , Bienhold Christina 
Published: July 2023

Marine sinking particles sequester atmospheric carbon dioxide to the deep oceanviathe biological carbon pump. Understanding how environmental shifts drive changes in the microbial composition of particles, and how these affect the export of organic matter from the surface to the deep ocean, is critical, especially in the rapidly changing Arctic Ocean. Here, we applied next generation sequencing of the 18S and 16S rRNA genes to sediment trap samples from around 200 m water depth in the eastern Fram Strait, covering a time frame of more than one decade (2000-2012). The aim was to characterize their microbial composition during annual highest particulate organic carbon flux events. The bimodal annual spring and summer export fluxes were representative of the strong seasonality in the region. Furthermore, the study period was characterized by considerable interannual variation, marked especially by a warm water anomaly between 2005 and 2007. During this period changes in the hydrography and sea ice cover also led to measurable changes in the microbial composition of particles. The warm water period was marked by a decrease in diatoms affiliated withChaetoceros, an increase of small phytoplankton and an increase in sequence abundance of the bacterial taxaOceanospirillales,AlteromonadalesandRhodobacteraleson the particles. The resulting changes in microbial composition and the associated microbial network structure suggest the emergence of a more developed retention system in the surface ocean. Our results provide the first long-term assessment of the microbial composition of sinking particles in the Arctic Ocean, and stress the importance of sea ice and hydrography for particle composition and subsequent flux of organic matter to deeper waters.
Failure of enhanced recovery after surgery in liver surgery: a systematic review and meta analysis
Authors: Ren Qiuping , Wu Menghang , Li Hong Yu , Li Jiafei , Zeng Zi Hang 
Published: July 2023

PurposeThis study aimed to conduct a systematic review of the literature to identify and summarize the existing evidence regarding ERAS failure and related risk factors after hepatic surgery. The objective was to provide physicians with a better understanding of these factors so that they can take appropriate action to minimize ERAS failure and improve patient outcomes.MethodA literature search of the PubMed MEDLINE, OVID, EMBASE, Cochrane Library, and Web of Science was performed. The search strategy involved terms related to ERAS, failure, and hepatectomy.ResultA meta-analysis was conducted on four studies encompassing a total of 1,535 patients, resulting in the identification of 20 risk factors associated with ERAS failure after hepatic surgery. Four of these risk factors were selected for pooling, including major resection, ASA classification of ≥3, advanced age, and male gender. Major resection and ASA ≥ 3 were identified as statistically significant factors of ERAS failure.ConclusionThe comprehensive literature review results indicated that the frequently identified risk factors for ERAS failure after hepatic surgery are linked to operative and anesthesia factors, including substantial resection and an American Society of Anesthesiologists score of 3 or higher. These insights will assist healthcare practitioners in taking prompt remedial measures. Nevertheless, there is a requirement for future high-quality randomized controlled trials with standardized evaluation frameworks for ERAS programs.
Maternal Obesity Related to High Fat Diet Induces Placenta Remodeling and Gut Microbiome Shaping That Are Responsible for Fetal Liver Lipid Dysmetabolism
Authors: Wang Ying-Wen , Yu Hong-Ren , Tiao Mao-Meng , Tain You-Lin , Lin I-Chun , Sheen Jiunn-Ming , Lin Yu-Ju , Chang Kow-Aung , Chen Chih-Cheng , Tsai Ching-Chou , Huang Li-Tung 
Published: July 2023

Background: Maternal obesity in utero may affect fetal development and cause metabolic problems during childhood and even adulthood. Diet-induced maternal obesity can impair gut barrier integrity and change the gut microbiome, which may contribute to adverse placental adaptations and increase the obesity risk in offspring. However, the mechanism through which maternal obesity causes offspring metabolic disorder must be identified.Methods: Eight-week-old female rats received a control diet or high-fat (HF) diet for 11 weeks before conception and during gestation. The placentas were collected on gestational day 21 before offspring delivery. Placental tissues, gut microbiome, and short-chain fatty acids of dams and fetal liver tissues were studied.Results: Maternal HF diet and obesity altered the placental structure and metabolism-related transcriptome and decreased G protein–coupled receptor 43 expression. HF diet and obesity also changed the gut microbiome composition and serum propionate level of dams. The fetal liver exhibited steatosis, enhanced oxidative stress, and increased expression of acetyl-CoA carboxylase 1 and lipoprotein lipase with changes in maternal HF diet and obesity.Conclusions: Maternal HF diet and obesity shape gut microbiota and remodel the placenta of dams, resulting in lipid dysmetabolism of the fetal liver, which may ultimately contribute to the programming of offspring obesity.
A reactive oxygen species–related signature to predict prognosis and aid immunotherapy in clear cell renal cell carcinoma
Authors: Liu Hongxiang , Luo Yong , Zhao Shankun , Tan Jing , Chen Minjian , Liu Xihai , Ye Jianheng , Cai Shanghua , Deng Yulin , Li Jinchuang , He Huichan , Zhang Xin , Zhong Weide 
Published: July 2023

BackgroundClear cell renal cell carcinoma (ccRCC) is a malignant disease containing tumor-infiltrating lymphocytes. Reactive oxygen species (ROS) are present in the tumor microenvironment and are strongly associated with cancer development. Nevertheless, the role of ROS-related genes in ccRCC remains unclear.MethodsWe describe the expression patterns of ROS-related genes in ccRCC from The Cancer Genome Atlas and their alterations in genetics and transcription. An ROS-related gene signature was constructed and verified in three datasets and immunohistochemical staining (IHC) analysis. The immune characteristics of the two risk groups divided by the signature were clarified. The sensitivity to immunotherapy and targeted therapy was investigated.ResultsOur signature was constructed on the basis of glutamate-cysteine ligase modifier subunit (GCLM), interaction protein for cytohesin exchange factors 1 (ICEF1), methionine sulfoxide reductase A (MsrA), and strawberry notch homolog 2 (SBNO2) genes. More importantly, protein expression levels of GCLM, MsrA, and SBNO2 were detected by IHC in our own ccRCC samples. The high-risk group of patients with ccRCC suffered lower overall survival rates. As an independent predictor of prognosis, our signature exhibited a strong association with clinicopathological features. An accurate nomogram for improving the clinical applicability of our signature was constructed. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes analyses showed that the signature was closely related to immune response, immune activation, and immune pathways. The comprehensive results revealed that the high-risk group was associated with high infiltration of regulatory T cells and CD8+ T cells and more benefited from targeted therapy. In addition, immunotherapy had better therapeutic effects in the high-risk group.ConclusionOur signature paved the way for assessing prognosis and developing more effective strategies of immunotherapy and targeted therapy in ccRCC.
PIWIL1 interacting RNA piR-017724 inhibits proliferation, invasion, and migration, and inhibits the development of HCC by silencing PLIN3
Authors: Wu Yi-Jing , Wang Jie , Zhang Peng , Yuan Liu-Xia , Ju Lin-Ling , Wang Hui-Xuan , Chen Lin , Cao Ya-Li , Cai Wei-Hua , Ni Yi , Li Min 
Published: July 2023

BackgroundHepatocellular carcinoma (HCC) accounts for the majority of primary liver cancers. Worldwide, liver cancer is the fourth most common cause of cancer-related death. Recent studies have found that PIWI-interacting RNAs (piRNAs) participate in the occurrence and development of various tumors and are closely related to the growth, invasion, metastasis and prognosis of malignant tumors. Studies on the role and functional mechanism of piRNAs in HCC development and progression are limited.MethodsQuantitative reverse transcription-polymerase chain reaction (qRT-PCR) were used to detect the expression of piR-017724 in both HCC tissues and cells. Based on the clinical data of HCC patients, the clinical and prognostic value of piR-017724 was further analyzed. Then, targeted silencing and overexpressing of piR-017724 in HCC cells was further used to examine the biological functions of piR-017724. In addition, the downstream target protein of piR-017724 was predicted and validated through high-throughput sequencing and public databases.ResultsThe piR-017724 was significantly downregulated in HCC tissues and cells, and the downregulation of piR-017724 was associated with tumor stage and poor prognosis in HCC. The piR-017724 inhibitor promoted the proliferation, migration and invasion of HCC cells, while the piR-017724 mimic had the opposite effect. However, the piR-017724 did not affect apoptosis of HCC cells. High-throughput sequencing and qRT-PCR confirmed a reciprocal relationship between piR-017724 and PLIN3. Therefore, we speculate that piR-017724 may inhibit the development and progression of HCC by affecting the downstream protein PLIN3.ConclusionsOur study shows that piR-017724, which is lowly expressed in HCC, inhibits the proliferation, migration and invasion of HCC cells and may affect the development of hepatocellular liver cancer through PLIN3, which provides new insights into the clinical application of piR-017724 in the treatment of hepatocellular carcinoma.