bluuebunny/crossref_metadata_2025_split huggingface dataset:
Anode-assisted electro-fermentation with Bacillus subtilis under oxygen-limited conditions
Authors: Sun Yu , Kokko Marika , Vassilev Igor 
Published: January 2023

Abstract
Background
Bacillus subtilis is generally regarded as a ubiquitous facultative anaerobe. Oxygen is the major electron acceptor of B. subtilis, and when oxygen is absent, B. subtilis can donate electrons to nitrate or perform fermentation. An anode electrode can also be used by microorganisms as the electron sink in systems called anodic electro-fermentation. The facultative anaerobic character of B. subtilis makes it an excellent candidate to explore with different electron acceptors, such as an anode. This study aimed to optimise industrial aerobic bioprocesses using alternative electron acceptors. In particular, different end product spectrum of B. subtilis with various electron acceptors, including anode from the electro-fermentation system, was investigated.

Results
B. subtilis was grown using three electron acceptors, i.e. oxygen, nitrate and anode (poised at a potential of 0.7 V vs. standard hydrogen electrode). The results showed oxygen had a crucial role for cells to remain metabolically active. When nitrate or anode was applied as the sole electron acceptor anaerobically, immediate cell lysis and limited glucose consumption were observed. In anode-assisted electro-fermentation with a limited aeration rate, acetoin, as the main end product showed the highest yield of 0.78 ± 0.04 molproduct/molglucose, two-fold higher than without poised potential (0.39 ± 0.08 molproduct/molglucose).

Conclusions
Oxygen controls B. subtilis biomass growth, alternative electron acceptors utilisation and metabolites formation. Limited oxygen/air supply enabled the bacteria to donate excess electrons to nitrate or anode, leading to steered product spectrum. The anode-assisted electro-fermentation showed its potential to boost acetoin production for future industrial biotechnology applications.
Brain correlates of declarative memory atypicalities in autism: a systematic review of functional neuroimaging findings
Authors: Desaunay Pierre , Guillery Bérengère , Moussaoui Edgar , Eustache Francis , Bowler Dermot M. , Guénolé Fabian 
Published: January 2023

AbstractThe long-described atypicalities of memory functioning experienced by people with autism have major implications for daily living, academic learning, as well as cognitive remediation. Though behavioral studies have identified a robust profile of memory strengths and weaknesses in autism spectrum disorder (ASD), few works have attempted to establish a synthesis concerning their neural bases. In this systematic review of functional neuroimaging studies, we highlight functional brain asymmetries in three anatomical planes during memory processing between individuals with ASD and typical development. These asymmetries consist of greater activity of the left hemisphere than the right in ASD participants, of posterior brain regions—including hippocampus—rather than anterior ones, and presumably of the ventral (occipito-temporal) streams rather than the dorsal (occipito-parietal) ones. These functional alterations may be linked to atypical memory processes in ASD, including the pre-eminence of verbal over spatial information, impaired active maintenance in working memory, and preserved relational memory despite poor context processing in episodic memory.
Medical and physiological complications of exercise for individuals with an eating disorder: A narrative review
Authors: Quesnel Danika A. , Cooper Marita , Fernandez-del-Valle Maria , Reilly Alanah , Calogero Rachel M. 
Published: January 2023

AbstractIndividuals with eating disorders (ED) experience prolonged malnutrition, binge episodes, and compensatory behaviours that affect every organ system. Psychological and physiological symptoms are worsened with comorbid dysfunctional exercise, seen in up to 80% of those with an ED. Although return to exercise is an important component of treatment and recovery, little is known about the contraindications and risks of exercise engagement specific to those with an ED. This paper provides a comprehensive narrative review of the medical and physiological complications of engaging in exercise during ED treatment and outlines when exercise may be contraindicated or used in modified or cautionary ways. We conducted a literature search on MEDLINE, PubMed, and PsychArticles to identify relevant articles, which yielded six categories of medical and physiological complications of ED that may be exacerbated by exercise: energy availability, cardiovascular health, electrolyte abnormalities, biomedical function markers, sex hormones, and body composition. We summarize the evidence for these complications for readers and offer an initial set of recommendations for incorporating exercise during ED treatment based on our findings. This review may serve as a resource for members of ED treatment teams to help evaluate more readily and confidently whether exercise is safe for individual patients and when modifications and caution may be warranted.
Cardioprotective effect of crude polysaccharide fermented by Trametes Sanguinea Lyoyd on doxorubicin-induced myocardial injury mice
Authors: Shen Chenjun , Yang Bo , Huang Lili , Chen Yueru , Zhao Huajun , Zhu Zhihui 
Published: January 2023

AbstractDoxorubicin (DOX) is a broad-spectrum anti-tumor drug, but its clinical application is greatly limited because of the cardiotoxicity. Thus, exploration of effective therapies against DOX-induced cardiotoxicity is necessary. The aim of this study is to investigate the effects and possible mechanisms of Trametes Sanguinea Lyoyd fermented crude polysaccharide (TSLFACP) against DOX-induced cardiotoxicity. We investigated the protective effects of TSLFACP on myocardial injury and its possible mechanisms using two in vitro cells of DOX-treated cardiomyocytes H9C2 and embryonic myocardial cell line CCC-HEH-2 and a in vivo mouse model of DOX-induced myocardial injury. We found that TSLFACP could reverse DOX-induced toxicity in H9C2 and CCC-HEH-2 cells. Similarly, we found that when pretreatment with TSLFACP (200 mg/kg, i.g.) daily for 6 days, DOX-induced myocardial damage was attenuated, including the decrease in serum myocardial injury index, and the amelioration in cardiac histopathological morphology. Additionally, immunohistochemistry and western blotting were used to identify the underlying and possible signal pathways. We found that TSLFACP attenuated the expression of LC3-II, Beclin-1 and PRAP induced by DOX. In conclusion, our results demonstrated that TSLFACP could protect against DOX-induced cardiotoxicity by inhibiting autophagy and apoptosis.
Cartas Ejecutorias de Hidalguía (executory certificates of nobility): a survey in materials analysis, legal, and aesthetic contexts—two case studies
Authors: Mercado-Oliveras Verónica , Alcántara-García Jocelyn 
Published: January 2023

Abstract
Illuminated manuscripts are relatively well studied, but the available publications greatly focus on religious manuscripts of a geographically limited area. In contrast, technical examinations of illuminated legal documents have received far less attention, e.g., Spanish cartas ejecutorias de hidalguía (executory certificates of nobility). These documents are suitable case studies to deepen current knowledge of manuscript-making in Spain for two reasons: First, they are dated (late fifteenth to early eighteenth centuries); and second, they are unusual and understudied from both the textual and materials analysis standpoints. Cartas contain judicial proceedings whereby a family gained or was re-assigned hidalguía (lower nobility). A key exhibit to achieving this status was proving their “blood purity” which implied they were faithful Catholics, so finding religious representations within the document is common. In addition, families embedded their faith and links to monarchs in their coat of arms through symbols like crowned eagles, trees, and towers. The deliberate choice of heraldic and religious elements is as important as the materials used to produce them. Interested in better understanding illumination in Spain we are studying these unique documents from the historical, materials, and iconographic points of view. Herein, we present the earliest results of an ongoing survey, detailing two case studies: Davila and Nuñez D. Armesto cartas. This research uses a combination of: (a) instrumental techniques (X-ray fluorescence, reflectance, and infrared spectroscopies; peptide mass fingerprinting; and gas chromatography); and (b) historical research using both the manuscript’s contents as primary sources, and published research. The preliminary results are enabling us to shed light onto Spanish (legal) illuminated manuscript-making, and the symbolic role materials played, e.g., use of precious metals adorning monarchical elements, presence of ultramarine mixed with azurite on both coats of arms, and on Virgin Mary’s gown, etc. This survey intends to simultaneously learn more about illumination practices in Spain, inform conservation decisions, and hopefully better understand problems connected to historic ideologies that were legalized in beautiful albeit disturbing documents, e.g., persecution of non-Catholics at the time in Spain.
The role of novelty stimuli in second language acquisition: evidence from the optimized training by the Pinyin Tutor at TalkBank
Authors: Zhang Yanhui , MacWhinney Brian 
Published: January 2023

AbstractAs hypothesized by the unified competition model (MacWhinney, 2007, 2017, 2021), optimizing training schemes can enhance second language (L2) learning by fostering various protective factors. Under such a framework, the current study focuses on how the familiarity of stimuli will affect learning Chinese phonetic skills in a computer-assisted language learning (CALL) environment. Two training conditions, i.e., training with familiar stimuli from the textbook and unfamiliar stimuli from novelty design, were administered for two groups of learners at American universities, where the classroom instructions were integrated with the Pinyin Tutor—an online spoken Chinese learning platform hosted under TalkBank. The results show that training with novelty stimuli leads to a greater pretest–posttest improvement for intermediate learners, whereas more significant improvement has been observed in training with familiar stimuli among beginning learners. The learning-enhancing power of the Pinyin Tutor is evidenced by the overall significance of the pretest–posttest improvement when consolidating the results of the two conditions. Furthermore, high retention has been demonstrated in all six aspects of the Pinyin knowledge as tested by a three-month-after delayed posttest. These findings tend to endorse a differentiated design of instructional materials with increasing novelty components as the level of L2 learning advances. The overall significant learning-boosting results accredit the design of the Pinyin Tutor, where the technological architecture and algorithms were integrated with psycholinguistic and pedagogical theories. Suggestions and implications for smart learning in general are presented.
Breakfast intake and associated factors and barriers among tertiary institution students in the Western Region, Ghana
Authors: Adonu Regina Enyonam , Amoah Millicent , Saah Farrukh Ishaque 
Published: January 2023

Abstract
Background
Breakfast is considered the day’s most important meal. Skipping breakfast consumption is detrimental to health and intellectual performance. University life has tight schedules and rigorous intellectual activities often very early in the morning. This study aimed at assessing breakfast intake and its associated factors among the students of Takoradi Technical University (TTU).

Methods
This was a descriptive cross-sectional study. Data was collected from 347 students in TTU using pre-tested questionnaire. Data collected were analysed using STATA v17. It employed descriptive and inferential statistics such as logistic regression. P-value less than 0.05 was set as statistical significance at 95% confidence interval.

Results
Regular breakfast was taken by 35.7% of the students. Higher odds of regular breakfast intake were found among respondents aged 25–29 years (AOR = 3.13, 95%CI = 1.57–6.24) and those who buy their breakfast (AOR = 5.13, 95%CI = 2.16–12.19). However, lower odds of regular breakfast consumption were found among respondents who were females (AOR = 0.40, 95%CI = 0.18–0.85). Barriers to regular breakfast intake included negative mood, insufficient funds, health condition, weight management, religious reasons such as fasting, limited time/unfavourable academic schedules, daily activities/workload, and cost of food on campus.

Conclusion
The study stressed on the need for external and personal factors such as sex, age, religion, limited time/unfavourable academic schedules among others that hinder habitual breakfast intake to be addressed adopting innovative approach such as peer education and campaigns. University management should implement favourable policies on academic schedules, canteens/cafeterias, less stringent regulation on cooking at halls of residence.
A socioeconomic approach to the profile of microcredit holders from the Hispanic minority in the USA
Authors: Cruz Rambaud Salvador , López Pascual Joaquín , Santandreu Emilio M. 
Published: January 2023

AbstractThe subject of this study is the microcredit market in the USA, more specifically in Florida. The justification for choosing this specific state is the massive presence of the Hispanic population. This will facilitate a generalization of the obtained results to the microcredit market in Latin American countries. Thus, the objective of this study is to analyze the profile of microcredit holders and their companies from socioeconomic and financial points of view. As our data also consider the degree of repayment of the microloans included in the sample, the clients’ profile is related to the punctuality or default of their corresponding loan repayments using the methodology of multinomial logit regression. The variables used in this study refer to personal information concerning borrowers (gender, age, education level, and marital status), the economic situation of their respective companies (closeness to the lender, number of workers, and revenues), and the characteristics of granted loans (principal, term, and purpose). However, the results of the regression show that only two variables are significant at the 5% significance level: the borrower’s age, which has a positive effect on repayment punctuality, and the loan term, which exhibits a negative effect. The findings of this study have clear implications, as they can help lenders design suitable microloans adjusted to customer profiles. Finally, future research should include other demographics and characteristics of affected companies.
Markets in crypto-assets regulation: Does it provide legal certainty and increase adoption of crypto-assets?
Authors: van der Linden Tina , Shirazi Tina 
Published: January 2023

AbstractThis study discusses the European Union’s proposal for a Regulation on Markets in Crypto-Assets, now subject to formal approval by the European Parliament. The objective is to explore whether it will positively impact the adoption of crypto-assets in the financial sector. The use of crypto-assets is growing. However, some stakeholders in the financial service sector remain skeptical and hesitant to adopt assets that are yet to be defined and have an unclear legal status. This regulatory uncertainty has been identified as the primary reason for the reluctant adoption. The proposed regulation (part of the EU’s Digital Finance Strategy) aims to provide this legal certainty for currently unregulated crypto-assets. This study investigates whether or not the proposed regulation can be expected to have the intended effect by reviewing the proposed regulation itself, the opinions and reactions of the various stakeholders, and secondary literature. Findings reveal that such regulation will most likely not accelerate the adoption of crypto-assets in the EU financial services sector, at least not sufficiently or as intended. Some suggestions are made to improve the proposal.
Simultaneous biportal endoscopic management of pineal region tumors in patients with obstructive hydrocephalus: technical notes
Authors: Al-Saiari Sultan , Al Orabi Khalid , Abdoh Mohammad Ghazi , Basurrah Abdulaziz A. , Albalawi Sultan Faez , Farag Ahmed A. 
Published: January 2023

Abstract
Background
The goal of this study is to show the feasibility and benefits of using the simultaneous biportal endoscopic procedure to treat pineal tumors in patients with obstructive hydrocephalus.

Methods
We retrospectively reviewed three patients with pineal tumors and acute obstructive hydrocephalus who were treated in one session with a frameless stereotactic guided simultaneous biportal endoscopic third ventriculostomy and endoscopic tumor biopsy performed through two separate ports using one rigid ventriculoscope.

Results
In the three patients, ventriculostomy and endoscopic biopsies were conducted. There was no death or morbidity throughout the 45-min procedure. All of the patients’ histological findings were confirmed. Germinoma was diagnosed in two patients who recieved postoperative radiotherapy, and the third patient diagnosed with a pineocytoma. Magnetic resonance imaging with flow-sensitive sequences was used to confirm ventriculostomy patency in all patients 6 months after the surgery.

Conclusion
Biportal endoscopic approach enables better visual control of both procedures. Furthermore, it allows the surgeon to safely pass the ventriculoscope via the foramen of monro, even if it is narrow. Moreover, during endoscopic tumor biopsy and third ventriculostomy, the intracranial pressure can be smoothly managed using the outlet tubes accessible. This treatment may be an alternative to traditional uniportal endoscopic operations in certain patients.
First genome-scale insights into the virulence of the snow mold causal fungus Microdochium nivale
Authors: Tsers Ivan , Marenina Ekaterina , Meshcherov Azat , Petrova Olga , Gogoleva Olga , Tkachenko Alexander , Gogoleva Natalia , Gogolev Yuri , Potapenko Evgenii , Muraeva Olga , Ponomareva Mira , Korzun Viktor , Gorshkov Vladimir 
Published: January 2023

AbstractPink snow mold, caused by a phytopathogenic and psychrotolerant fungus, Microdochium nivale, is a severe disease of winter cereals and grasses that predominantly occurs under snow cover or shortly after its melt. Snow mold has significantly progressed during the past decade, often reaching epiphytotic levels in northern countries and resulting in dramatic yield losses. In addition, M. nivale gradually adapts to a warmer climate, spreading to less snowy territories and causing different types of plant diseases throughout the growing period. Despite its great economic importance, M. nivale is poorly investigated; its genome has not been sequenced and its crucial virulence determinants have not been identified or even predicted. In our study, we applied a hybrid assembly based on Oxford Nanopore and Illumina reads to obtain the first genome sequence of M. nivale. 11,973 genes (including 11,789 protein-encoding genes) have been revealed in the genome assembly. To better understand the genetic potential of M. nivale and to obtain a convenient reference for transcriptomic studies on this species, the identified genes were annotated and split into hierarchical three-level functional categories. A file with functionally classified M. nivale genes is presented in our study for general use. M. nivale gene products that best meet the criteria for virulence factors have been identified. The genetic potential to synthesize human-dangerous mycotoxins (fumonisin, ochratoxin B, aflatoxin, and gliotoxin) has been revealed for M. nivale. The transcriptome analysis combined with the assays for extracellular enzymatic activities (conventional virulence factors of many phytopathogens) was carried out to assess the effect of host plant (rye) metabolites on the M. nivale phenotype. In addition to disclosing plant-metabolite-upregulated M. nivale functional gene groups (including those related to host plant protein destruction and amino acid metabolism, xenobiotic detoxication (including phytoalexins benzoxazinoids), cellulose destruction (cellulose monooxygenases), iron transport, etc.), the performed analysis pointed to a crucial role of host plant lipid destruction and fungal lipid metabolism modulation in plant-M. nivale interactions.
Radiographic measurement of the congruence angle according to Merchant: validity, reproducibility, and limits
Authors: Severyns M. , Mallet J. , Santoni B. , Barnavon T. , Germaneau A. , Vendeuvre T. , Drame M. 
Published: January 2023

Abstract
Purpose
The objective of this study was to analyze the intra- and interobserver variability of this measurement according to a strict methodology and on a representative sample of the general population, as well as to identify the possible difficulties of measurement in case of patellar or trochlear dysplasia.

Methods
This observational study involved radiographic analysis by three independent observers of a total of 50 patients who had a loaded patellofemoral X-ray taken with the knee flexed to 45°. An initial reading was taken to measure the angle of the trochlear sulcus, the Merchant angle, and to classify the knees according to a possible trochlear dysplasia and/or patellar dysplasia according to Wiberg. A second measurement was then performed to analyze intraobserver agreement. Interobserver agreement was measured on all radiographic measurements (n = 100).

Results
The Merchant patellofemoral congruence angle showed good intraobserver concordance ranging from 0.925 (95% CI 0.868–0.957) to 0.942 (95% CI 0.898–0.967), as well as interobserver concordance ranging from 0.795 (95% CI 0.695–0.862) to 0.914 (95% CI 0.872–0.942). Poor results were found in terms of interobserver concordance on the measurement of the Merchant angle in case of stage 3 Wiberg patella ranging from 0.282 (95% CI −0.920 to 0.731) to 0.611 (95% CI 0.226–0.892).

Conclusion
Congruence angle is one of most commonly used measurements for patellar tracking. However, the convexity of the patellar surface makes it difficult to identify the patellar apex on its intraarticular facet, making the measurement of the Merchant congruence angle unreliable and not very reproducible in cases of stage 3 Wiberg patella.
Registration N°IRB 2021/139