bluuebunny/crossref_metadata_2025_split huggingface dataset:
LM-DTI: a tool of predicting drug-target interactions using the node2vec and network path score methods
Authors: Li Jianwei , Wang Yinfei , Li Zhiguang , Lin Hongxin , Wu Baoqin 
Published: May 2023

Introduction: Drug-target interaction (DTI) prediction is a key step in drug function discovery and repositioning. The emergence of large-scale heterogeneous biological networks provides an opportunity to identify drug-related target genes, which led to the development of several computational methods for DTI prediction.Methods: Considering the limitations of conventional computational methods, a novel tool named LM-DTI based on integrated information related to lncRNAs and miRNAs was proposed, which adopted the graph embedding (node2vec) and the network path score methods. First, LM-DTI innovatively constructed a heterogeneous information network containing eight networks composed of four types of nodes (drug, target, lncRNA, and miRNA). Next, the node2vec method was used to obtain feature vectors of drug as well as target nodes, and the path score vector of each drug-target pair was calculated using the DASPfind method. Finally, the feature vectors and path score vectors were merged and input into the XGBoost classifier to predict potential drug-target interactions.Results and Discussion: The 10-fold cross validations evaluate the classification accuracies of the LM-DTI. The prediction performance of LM-DTI in AUPR reached 0.96, which showed a significant improvement compared with those of conventional tools. The validity of LM-DTI has also been verified by manually searching literature and various databases. LM-DTI is scalable and computing efficient; thus representing a powerful drug relocation tool that can be accessed for free at http://www.lirmed.com:5038/lm_dti.
How about the evidence assessment tools used in education and management systematic reviews?
Authors: Lan Hui , Yu Xuan , Wang Zhe , Wang Ping , Sun Yajia , Wang Zijun , Su Renfeng , Wang Ling , Zhao Junxian , Hu Yue , Wu Shouyuan , Ren Mengjuan , Yang Kehu , Liu Xingrong , Chen Yaolong 
Published: May 2023

ObjectivesTo systematically analyze the use of evidence assessment tools in systematic reviews of management and education.Study design and settingWe systematically searched selected literature databases and websites to identify systematic reviews on management and education. We extracted general information of the included studies and information about the evidence assessment tool they applied, including whether it was used for methodological quality assessment, reporting quality assessment or evidence grading, as well as the name, reference, publication year, version and original intended use of the tool, the role of the tool in the systematic review, and whether the quality determination criteria were given.ResultsA total of 299 systematic reviews were included, of which only 34.8% used evidence assessment tools. A total of 66 different evidence assessment tools were used, of which Risk of Bias (ROB) and its updated version (n = 16, 15.4%) were the most frequent. The specific roles of the evidence assessment tools were reported clearly in 57 reviews, and 27 reviews used two tools.ConclusionEvidence assessment tools were seldom used in systematic reviews in social sciences. The understanding and reporting of evidence assessment tools among the researchers and users still needs improvement.
Effects of dietary N-carbamylglutamate on rumen fermentation parameters, and bacterial community diversity of Holstein dairy cows in Tibet
Authors: Zhu Jinjia , Wu Yicheng , Jiang Aoyu , Li Bin , Ran Tao , Zhou Chuanshe , Xiao Dingfu , Tan Zhiliang 
Published: May 2023

IntroductionThe Tibetan Plateau is characterized by low temperature and hypoxia. N-carbamylglutamic acid (NCG) can increase blood oxygen saturation, and have the potential to be used to prevent the high-altitude hypoxia stress state of cows. However, its beneficial effect on the rumen microbiota of Holstein dairy cows remains unclear.MethodsHence, the experiments 12 multiparous (parity ranged from 2 to 7) Holstein dairy cows (413.0 ± 42 kg) were randomly assigned to 2 treatments with 6 replicates in each treatment: basal diet (CON, control group) and basal diet plus 20 g/d/cow of NCG (NCG, experiment group), respectively. To study the effects of dietary NCG supplementation on rumen microbiota of Holstein dairy cows in Tibet. The experiment lasted for 45 days, with 15 days of pre-feeding and 30 days of formal trail period.ResultsThe results showed that ruminal NH3-N concentration in NCG group was lower (p &lt; 0.05) than that in the CON group, while molar proportion of acetic acid and total volatile fatty acid (VFA) concentration were increased (p &lt; 0.05) with the addition of NCG. Microbial diversity increased (p &lt; 0.05) in NCG group, with Bacteroidetes, Firmicutes, and Patescibacteria as the most abundant phyla. The KEGG pathway analysis showed that the potential function of ruminal bacteria was mainly enriched in metabolism (carbohydrates, amino acids, lipids, energy, and nucleotides) and genetic information processing (replication, repair, and translation).ConclusionIn conclusion, NCG can improve rumen nitrogen utilization, total VFA and acetic acid production, and increase rumen microbial diversity, all of which could make the introduced Holstein dairy cows to better adapt to the harsh environment in Tibet and improve their production performance.
Altered hemispheric asymmetry of attentional networks in patients with pituitary adenoma: an event-related potential study
Authors: Wang Shuochen , Fu Zhenghao , Sun Yuanyi , Zhang Meng , Chen Aobo , Cao Chenglong , Song Jian 
Published: May 2023

BackgroundEmerging evidence has been reported of attentional dysfunction in pituitary adenoma patients. However, the effect of pituitary adenomas on lateralized attention network efficiency remained to be clear. Thus, the present study aimed to investigate the impairment of lateralized attention networks in patients with pituitary adenoma.MethodsEighteen pituitary adenoma patients (PA group) and 20 healthy controls (HCs) were included in this study. Both behavioral results and event-related potentials (ERPs) were acquired while subjects performed the Lateralized Attention Network Test (LANT).ResultsBehavioral performances indicated the PA group had a slower reaction time and a similar error rate relative to the HCs group. Meanwhile, significantly increased executive control network efficiency suggested the dysfunction of inhibition control in PA patients. Regarding ERP results, there were no group differences in the alerting and orienting networks. The target-related P3 was significantly reduced in the PA group, suggesting an impairment of executive control function and attentional resources allocation. Moreover, the mean amplitude of P3 was significantly lateralized to the right hemisphere, and interacted with the visual field, exhibiting that the right hemisphere dominated the bilateral visual field, whereas the left hemisphere dominated the left visual field. In the specific high-conflict condition, the pattern of hemispheric asymmetry in the PA group was altered due to a mixed effect resulting from the compensatory recruitment of attentional resources in the left central parietal area and the destructive effects of hyperprolactinemia.ConclusionThese findings suggested that, in the lateralized condition, the decreased P3 in the right central parietal area and the diminished hemispheric asymmetry under high conflict load, may serve as the potential biomarkers of attentional dysfunction in patients with pituitary adenoma.
Adipose tissue-derived exosomes contribute to obesity-associated liver diseases in long-term high-fat diet-fed mice, but not in short-term
Authors: Son Taesang , Jeong Inae , Park Jeongjin , Jun Woojin , Kim Andre , Kim Ok-Kyung 
Published: May 2023

IntroductionOur study aimed to investigate the changes in hepatic endoplasmic reticulum (ER) stress, inflammation, insulin signaling, and lipid metabolism during the administration of a high-fat diet (HFD) in mice in order to identify correlations between obesity and metabolic disease development in the liver.MethodsWe used short-, medium-, and long-term HFD periods, corresponding to 4, 8, and 12 weeks, respectively, and isolated exosomes from adipose tissue. We confirmed the effect of adipose tissue-derived exosomes on metabolic disorders in obesity in alpha mouse liver 12 (AML12) hepatocytes.ResultsAdipose tissue-derived exosomes from HFD mice did not affect the AML12 cells after 4 weeks, but ER stress, inflammatory response, insulin resistance, and lipid synthesis were observed after 8 and 12 weeks. Furthermore, we confirmed that an HFD increases the amount of adipose tissue-derived exosomes in mice. Consequently, we can infer that adipose tissue-derived exosomes from HFD-fed mice significantly increase ER stress, inflammatory response, insulin resistance, and lipid synthesis in AML12 cells.DiscussionOur results demonstrate that obesity alters the effects of adipose tissue-derived exosomes in the liver, potentially becoming a risk factor in the development of obesity-induced liver diseases.
A longitudinal study of fatty acid profiles, macronutrient levels, and plasmin activity in human milk
Authors: Meng Fanyu , Uniacke-Lowe Therese , Lanfranchi Elisa , Meehan Grainne , O'Shea Carol-Anne , Dennehy Theresa , Ryan Anthony C. , Stanton Catherine , Kelly Alan L. 
Published: May 2023

IntroductionHuman milk provides nutrients essential for infant growth and health, levels of which are dynamic during lactation.MethodsIn this study, changes in macronutrients, fatty acids, and plasmin activities over the first six months of lactation in term milk were studied.ResultsThere was a significant influence of lactation stage on levels of protein and plasmin activities, but not on levels of fat and carbohydrate in term milk. Concerning fatty acids in term milk, levels of caproic acid and α-linolenic acid increased significantly (p &lt; 0.05), whereas those of arachidonic acid and docosahexaenoic acid decreased, in the six months after birth. Significant impacts of maternal pre-pregnancy BMI and infant gender on fatty acid profiles were also found. Multivariate statistical analysis showed that protein level, plasmin activity, and several fatty acids (α-linolenic acid, lignoceric acid, and docasadienoic acid) contributed strongly to discrimination of milk from different lactational stages.DiscussionThe study demonstrates that not all but some fatty acids were influenced by lactation, whereas protein and protease levels showed clear decreasing trends during lactation, which may help in understanding the nutritional requirements of infants.
Establishment, characterization, and drug screening of low-passage patient individual non-small cell lung cancer in vitro models including the rare pleomorphic subentity
Authors: Andus Ingo , Prall Friedrich , Linnebacher Michael , Linnebacher Christina S. 
Published: May 2023

IntroductionFor pre-clinical drug development and precision oncology research, robust cancer cell models are essential. Patient-derived models in low passages retain more genetic and phenotypic characteristics of their original tumors than conventional cancer cell lines. Subentity, individual genetics, and heterogeneity greatly influence drug sensitivity and clinical outcome.Materials and methodsHere, we report on the establishment and characterization of three patient-derived cell lines (PDCs) of different subentities of non-small cell lung cancer (NSCLC): adeno-, squamous cell, and pleomorphic carcinoma. The in-depth characterization of our PDCs included phenotype, proliferation, surface protein expression, invasion, and migration behavior as well as whole-exome and RNA sequencing. Additionally, in vitro drug sensitivity towards standard-of-care chemotherapeutic regimens was evaluated.ResultsThe pathological and molecular properties of the patients’ tumors were preserved in the PDC models HROLu22, HROLu55, and HROBML01. All cell lines expressed HLA I, while none were positive for HLA II. The epithelial cell marker CD326 and the lung tumor markers CCDC59, LYPD3, and DSG3 were also detected. The most frequently mutated genes included TP53, MXRA5, MUC16, and MUC19. Among the most overexpressed genes in tumor cells compared to normal tissue were the transcription factors HOXB9, SIM2, ZIC5, SP8, TFAP2A, FOXE1, HOXB13, and SALL4; the cancer testis antigen CT83; and the cytokine IL23A. The most downregulated genes on the RNA level encode the long non-coding RNA LANCL1-AS1, LINC00670, BANCR, and LOC100652999; the regulator of angiogenesis ANGPT4; the signaling molecules PLA2G1B and RS1; and the immune modulator SFTPD. Furthermore, neither pre-existing therapy resistances nor drug antagonistic effects could be observed.ConclusionIn summary, we successfully established three novel NSCLC PDC models from an adeno-, a squamous cell, and a pleomorphic carcinoma. Of note, NSCLC cell models of the pleomorphic subentity are very rare. The detailed characterization including molecular, morphological, and drug-sensitivity profiling makes these models valuable pre-clinical tools for drug development applications and research on precision cancer therapy. The pleomorphic model additionally enables research on a functional and cell-based level of this rare NCSLC subentity.
Continuous IV infusion of anakinra
Authors: Saunders Brittney N. , Kuijpers Marcela V. , Sloan Jason J. , Gertner Elie 
Published: May 2023

Objective: A review of the use of continuous IV infusion of anakinra; a description of the protocol for continuous IV infusion of anakinra in the treatment of cytokine storm developed over the past 4 years at a tertiary level academic medical center in the United States.Methods: We reviewed published reports of continuous IV infusion of anakinra in cytokine storm and summarize this method of treatment in other diseases. As well, over the past 4 years, continuous IV infusions of anakinra were administered at our tertiary level academic medical center in the United States (Regions Hospital, St. Paul, Minnesota) for approximately 400 patient days of treatment primarily for the cytokine storm associated with macrophage activation syndrome (MAS) in adults. This updated protocol is presented. While this a single center protocol, it may serve as an initial guide for further refinement of protocols in MAS and other conditions.Conclusion:Continuous IV infusion of anakinra has advantages over subcutaneous infusions and may be important in controlling severe life-threatening cytokine storm as seen in macrophage activation syndrome. This has the potential to be an important therapy for other syndromes including Cytokine Release Syndrome related to CAR T-cell therapy. Close collaboration between Rheumatology, Pharmacy and Nursing allows this treatment to be delivered rapidly and efficiently.
Effects of “accurate measurement” comprehensive sports activities on balance ability, body composition and bone density of female college students
Authors: Zhang Zhilei , Liu Jie , Li Jianguo , Li Jianping 
Published: May 2023

Background: A sedentary lifestyle with little movement has affected modern youth, and regular exercise has real benefits for people; such studies are mostly for older adults, and more evidence is needed for adolescents.Objective: To compare differences in balance, body composition, and bone mineral density among female college students before and after an exercise intervention to provide precise evidence that exercise promotes college student health.Methods: A whole group of female students in a university was sampled and included in the statistical analysis 50 people, divided into two cohorts, 21 people in the test group and 29 people in the control group; the test group had 4 comprehensive sports activities per week and the control group had 1 comprehensive sports activities per week, and the differences in each index of balance ability, body composition and bone density before and after the intervention were compared after 3 months.Results: After exercise intervention, when maintaining balance, the area of the center of gravity movement trajectory increased by 32.36% in the test group compared with the pre-intervention period and increased by 42.80% compared with the control group, and the differences were all statistically significant (p &lt; 0.01); body mass index (BMI), body fat rate (BFR), visceral fat area (VFA), skeletal muscle content, and Inbody score increased over time more reasonable, and the difference in the effect of time factor (effect) was statistically significant (p &lt; 0.01); bone mineral density (BMD) and BMD Z value increased with time, and the difference in the effect of time factor was statistically significant (p &lt; 0.05).Conclusion: Female college students’ body balance ability improved substantially after exercise intervention; at the university level, female college students had a more rational body composition and continued natural increase in BMD, which were not related to exercise intervention.
Exercise adherence and suicidal ideation of Chinese college students: a chain mediation model test
Authors: Xing Zhi , Guo Kelei , Hui Zhen , Ma Qishuai 
Published: May 2023

ObjectiveThe purpose of this study was to explore the relationship between exercise adherence and suicidal ideation in college students, as well as the mediating role of meaning in life and internet addiction.MethodsA total of 1925 college students (Mage = 19.51 years, SDage = 2.393 years) were recruited by stratified cluster sampling method in Zhaoqing University, among which 890 were males and 1,035 were females. Exercise adherence, meaning in life, internet addiction and suicidal ideation were assessed by using standard scales. Data were analyzed by Pearson Correlation Analysis, and bias-correction percentile Bootstrap method.Results(1) There is a significant correlation between exercise adherence, meaning in life, internet addiction and suicide ideation; (2) meaning in life plays a significant mediating role between exercise adherence and suicidal ideation; internet addiction plays a significant mediating role between exercise adherence and suicidal ideation; meaning in life and internet addiction play a chain mediating role between exercise adherence and suicide ideation.ConclusionExercise adherence can not only directly predict college students’ suicidal ideation, but also indirectly predict college students’ suicidal ideation through the independent mediation and chain mediation of meaning in life and internet addiction.
Antiretroviral treatment interruption and resumption within 16 weeks among HIV-positive adults in Jinan, China: a retrospective cohort study
Authors: Ma Jing , Jin Yan , Jiao Kedi , Wang Yao , Gao Lijie , Li Xinrui , Ma Wei 
Published: May 2023

BackgroundTreatment interruption has been found to increase the risk of opportunistic infections and death among HIV-positive adults, posing a challenge to fully realizing antiretroviral therapy (ART). However, it has been observed that short-term interruption (&lt;16 weeks) was not associated with significant increases in adverse clinical events. There remains a dearth of evidence concerning the interruption and resumption of ART after short-term discontinuation in China.MethodsHIV-positive adults who initiated ART in Jinan between 2004 and 2020 were included in this study. We defined ART interruption as more than 30 consecutive days off ART and used Cox regression to identify predictors of interruption. ART resumption was defined as a return to ART care within 16 weeks following discontinuation, and logistic regression was used to identify barriers.ResultsA total of 2,506 participants were eligible. Most of them were male [2,382 (95%)] and homosexual [2,109 (84%)], with a median age of 31 (IQR: 26–40) years old. Of all participants, 312 (12.5%) experienced a treatment interruption, and the incidence rate of interruption was 3.2 (95% CI: 2.8–3.6) per 100 person-years. A higher risk of discontinuation was observed among unemployed individuals [adjusted hazard ratio (aHR): 1.45, 95% CI: 1.14–1.85], with a lower education level (aHR: 1.39, 95% CI: 1.06–1.82), those with delayed ART initiation (aHR: 1.43, 95% CI: 1.10–1.85), receiving Alafenamide Fumarate Tablets at ART initiation (aHR: 5.19, 95% CI: 3.29–8.21). About half of the interrupters resumed ART within 16 weeks, and participants who delayed ART initiation, missed the last CD4 test before the interruption and received the “LPV/r+NRTIs” regimen before the interruption were more likely to discontinue treatment for the long term.ConclusionAntiretroviral treatment interruption remains relatively prevalent among HIV-positive adults in Jinan, China, and assessing socioeconomic status at treatment initiation will help address this issue. While almost half of the interrupters returned to care within 16 weeks, further focused measures are necessary to reduce long-term interruptions and maximize the resumption of care as soon as possible to avoid adverse clinical events.
A scoping review of maternal health among resettled refugee women in the United States
Authors: Yeo Sarah , Park Yuae , McClelland Deborah Jean , Ehiri John , Ernst Kacey , Magrath Priscilla , Alaofè Halimatou 
Published: May 2023

BackgroundGlobally, refugee women continue to face higher maternity-related risks from preventable complications during pregnancy and childbirth, partly due to high health care costs, unfamiliarity with the healthcare system, language barriers, and discrimination. Nevertheless, there is still a paucity of literature that evaluates the available evidence in the US. This scoping review delineated the body of literature on maternal health among refugee women resettled in the US in order to identify knowledge gaps in the literature and highlight future research priorities and directions for maternal health promotion.MethodsElectronic databases were searched in PubMed, CINAHL, PsycINFO, and EMBASE from inception through July 2021. We included all peer-reviewed study designs; qualitative, quantitative, and mixed method if they reported on refugee women's perinatal health experiences and outcomes in the US.ResultsA total of 2,288 records were identified, with 29 articles meeting the inclusion criteria. Refugee women tend to initiate prenatal care late and have fewer prenatal care visits compared to women born in the US. Some of them were reluctant to get obstetric interventions such as labor induction and cesarean delivery. Despite numerous risk factors, refugee women had generally better maternal health outcomes. Studies have also highlighted the importance of health care providers' cultural competency and sensitivity, as well as the potential role of community health workers as a bridge between refugee women and health care providers.ConclusionsThe scoping review emphasizes the need for early prenatal care initiation and more frequent prenatal care visits among refugee women. Furthermore, more needs to be done to mitigate resistance to obstetric interventions and mistrust. The mechanism by which healthy migrant effects occur could be better understood, allowing protective factors to be maintained throughout the resettlement and acculturation process. The scoping review identifies critical gaps in the literature, such as the underrepresentation of different ethnic groups of refugee women in refugee maternal studies in the US. Since this invisibility may indicate unspoken and unaddressed needs, more attention should be paid to underrepresented and understudied groups of refugee women in order to achieve health equity for all.