bluuebunny/crossref_metadata_2025_split huggingface dataset:
Ocular Myastheia Gravis as the First Presentation of Myastheia Gravis: A Case Report
Authors: Ma Chunhui , Chen Chunwen , Yao Jingyan 
Published: August 2023

Abstract
Background
Myasthenia gravis (MG) is a rare and potentially dangerous autoimmune neuromuscular disease, which affects the acetylcholine receptors at the neuromuscular junction of skeletal muscle. lt is characterized by fluctuating weakness and fatigue of the ocular, bulbar, limb, and respiratory muscles and initial symptoms are usually ptosis and diplopia. Over half of patients with MG will initially experience isolated ocular symptoms in one eye or both eyes, which are called ocular myasthenia gravis (OMG). OMG’s diverse symptoms may readily masquerade as other neurological conditions, posing a diagnostic challenge to clinicians.
Case presentation:
A 49-year-old female presented to the Emergency Department with a seven-day history of unilateral persistent ptosis with inflexible eye movement, concerning for oculomotor nerve paralysis initially. However, cranial CTA found no evidence of stroke or any other central etiology. Routine laboratory testing was unremarkable. Neurology was consulted and neurologist recommended sending tensilon testing (pharmacologically induced improvement in symptoms using the anticholinesterase inhibitor) and electromyographic testing. At the patient’s subsequent hospitalization in neurology department, these tests were found to be abnormal. The patient underwent complete ophthalmic examination and medical history review, was diagnosed of ocular myasthenia gravis (OMG) eventually. The patient subsequently underwent hormone and immunosuppressive therapy with obvious improvement in her symptoms.
Conclusions
MG may present as unilateral ptosis or diplopia with the hallmark characteristic of fluctuating muscle weakness. Early diagnosis and subsequent treatment of MG improve long term prognosis and remission rates. Prednisone is the most commonly used immune modulator for the treatment of MG. Emergency physicians should consider myasthenia gravis in cases of ocular symptoms after ruling-out emergent central etiologies.
Early Choroidal and Retinal Changes Detected by Swept-Source Oct in Type 2 Diabetes and Their Association with Diabetic Kidney Disease: A Longitudinal Prospective Study
Authors: Silva Monica Oliveira , Chaves Anne Elise Cruz do Carmo , Gobbato Glauber Corrêa , Lavinsky Fabio , Lavinsky Daniel 
Published: August 2023

Abstract
Background
To evaluate structural changes in retina and choroid in patients with type 2 diabetes (T2D) and their association with diabetic kidney disease (DKD)
Methods
T2D patients with mild or no diabetic retinopathy (DR) were followed for 3 years using structural SS-OCT and OCT angiography (OCT-A) taken every 6 months. Parameters were compared longitudinally and according to the DKD status on baseline.
Results
One hundred and sixty eyes from 80 patients were followed for 3 years, 72 with no DKD (nDKD) at baseline and 88 with DKD. Trend analysis of T2D showed significant thinning in GCL + and circumpapillary retinal fiber neural layer (cRFNL), choroid, and decreased vascular density (VD) in superficial plexus and central choriocapillaris with foveal avascular zone (FAZ) enlargement. Patients with no DKD on baseline presented more significant declines in retinal center and choroidal thickness, increased FAZ and loss of nasal and temporal choriocapillaris volume. In addition, the nDKD group had worse glycemic control and renal parameters at the end of the study.
Conclusion
Our data suggest that early, progressive neurovascular damage occurs in the retina and choroid in T2D patients with no or mild DR. Glycemic control and renal damage parameters were associated to neurovascular damage progression.
The Spindle Approximation of Network Epidemiological Modeling
Authors: Lu Xin , Mou Jianhon , Dai Bitao , Tan Suoyi , Holme Petter , Lehmann Sune , liljeros Fredrik 
Published: August 2023

Abstract
Understanding the dynamics of spreading and diffusion on networks is of critical importance for a variety of processes in real life. However, predicting the temporal evolution of diffusion on networks remains challenging as the process is shaped by network topology, spreading non-linearities, and heterogeneous adaptation behavior. In this study, we propose the ‘spindle vector’, a new network topological feature, which characterizes the hierarchical organization of nodes. The spindle vector shapes nodes according to the distance from the root node, capturing the essence of diffusion propagation, thus allowing us to approximate the spatiotemporal evolution of diffusion dynamics on networks. Through experiments on various networks, we show that our method outperforms the state-of-the-art, such that the prediction error of RMSE and MAE is 100% superior on WS and BA networks, and that the prediction is better than the counterpart model in 36 out of 40 empirical networks. The new metric provides a general and computationally efficient approach to predict network diffusion problems and is of potential for a large range of network applications.
Paclitaxel-Induced Inhibition of NSCLC Invasion and Migration via RBFOX3-Mediated circIGF1R Biogenesis
Authors: Xu Zhanyu , Zheng Liping , Li Shikang 
Published: August 2023

Abstract
Background: We previously reported that circIGF1R is significantly downregulated in non-small cell lung cancer (NSCLC) cells and tissues. It inhibits cancer cell invasion and migration, although the underlying molecular mechanisms remain elusive.
Methods: The invasion and migration of NSCLC cells was analyzed by routine in vivo and in vitro functional assays. Fluorescent in situ hybridization, luciferase reporter assay, RNA pull-down assay and RNA immunoprecipitation (RIP) assay were performed to explore the molecular mechanisms. Mechanism of action of paclitaxel-induced RBFOX3-mediated inhibition of NSCLC invasion and migration was investigated through in vitro and in vivo experiments.
Results: We found that circIGF1R regulated Van-Gogh-like 2 (VANGL2) expression as a Competing endogenous RNA (ceRNA) by “sponging” miR-1270, and inhibited the invasion and migration progression of NSCLC cells by inhibiting the Wnt pathway via the miR-1270/VANGL2 axis. Furthermore, RNA binding protein fox-1 homolog 3 (RBFOX3) promoted circIGF1R biogenesis through binding to the IGF1R pre-mRNA. RBFOX3 inhibits the migration and invasion ability of PC9 cells and A549 cells by increasing the biogenesis of circIGF1R. The chemotherapeutic drug paclitaxel inhibited NSCLC invasion and migration by inducing RBFOX3-mediated circIGF1R biogenesis.
Conclusions: RBFOX3 inhibits the invasion and migration of NSCLC cells through the circIGF1R/ miR-1270/VANGL2 axis, circIGF1R has the potential to serve as a biomarker and therapeutic target for NSCLC.
Suppression of neo-intimal hyperplasia induced by arteriovenous anastomosis and balloon-injury in rat by multimeric TRAIL
Authors: Kim Tae-Hyoung 
Published: August 2023

Abstract
Tumor necrosis factor-related apoptosis inducing factor (TRAIL) has shown to play a key role in tumor surveillance, autoimmune diseases, and apoptosis, but its role in vascular stenosis is controversial. In this study, the recombinant hexameric soluble TRAIL (referred as ILz(6):TRAIL), which contains the 6xHis tag, the isoleucine zipper hexamerization domain (ILz), and the extracellular region of TRAIL, inhibits the proliferation of SMemb+/CRBP-1+ vascular smooth muscle cells induced by anastomosis of a carotid artery and a jugular vein (AAV) or balloon injury of femoral artery in rats. Treatment with recombinant ILz(6):TRAIL significantly inhibited the occlusive progress of neo-intimal hyperplasia induced by AAV in a dosage-dependent manner, and adenovirus expressing secretable ILz(6):TRAIL also inhibits neo-intima hyperplasia induced by balloon injury in femoral artery of rats. Furthermore, this inhibitory effect of recombinant ILz(6):TRAIL was associated with the up-regulation of active caspase-3 in vascular smooth muscle cells in AAV regions. Surprisingly, the occlusions of vessels induced by AAV was partially reversed by recombinant ILz(6):TRAIL. This results demonstrates the preventive and partial regressive effects of ILz(6):TRAIL on AAV- or balloon-induced neo-intimal hyperplasia.
Relationships between statins and risk reduction of aortic dissection in patients with hypertension
Authors: Dang Yujie , Li Nanfang , Zhu Qing , Liu Shasha , Heizhati Mulalibieke , Gan Lin , Cai Xintian , Wen Wen , Wang Menghui , Hu Junli , Zhang Delian , Hong Jing , Luo Qin 
Published: August 2023

Abstract
Purpose
Statins have cardiovascular protective effects in addition to lipid-lowering effects. However, no human studies have examined whether statins prevent aortic dissection. This study aimed to explore the association between statins and aortic dissection.
Methods
This nested case–control study was based on data extracted from the UHDATA (Hypertension Database at Urumchi) in Xinjiang of China. Cases (patients who developed aortic dissection) and controls (patients without aortic dissection; matched for age, sex, and date of aortic dissection diagnosis) were selected from among the 52,146 adult patients with hypertension and hyperlipidemia or high-risk hypertension registered in the database between January 1, 2006, and December 31, 2018. Follow-up data were collected up to April 30, 2022. Multivariable logistic regression analysis was used to assess the relationship between statin use and aortic dissection.
Results
A total of 7049 patients (75.6% men; mean age, 54.6 years) were selected for the study: 647 patients who developed aortic dissection during the follow-up period and 6402 patients who did not develop aortic dissection. The proportion of patients using statins was higher in the case group than in the control group (21.2% vs. 29.9%, P < 0.001). However, in multivariable logistic regression analysis, statin use was independently associated with decreased risk for aortic dissection (adjusted OR = 0.538, 95% CI: 0.418–0.692, P < 0.001).
Conclusions
Statins appear to reduce risk of aortic dissection, and clinicians should consider early use of statins in hypertensive patients, especially those with hyperlipidemia and multiple risk factors.
Large-Scale Synthesis of Defect-Free Phosphorene on Nickel Substrates: Enabling Atomistic Thickness Devices.
Authors: Tchoffo Brice , Benabdallah ismail , Aberda abderrahman , Neugebauer Petr , Belhboub anouar , fatimy abdelouahad el 
Published: August 2023

Abstract
The synthesis of low-dimensional materials using phosphorus atoms remains challenging and extensively researched. Among these materials, phosphorene, a monolayer of black phosphorus, shows great promise for applications in atomically thin transistors and photonic devices. However, the lack of a reliable defect-free large-scale synthesis method has hindered its development for practical devices. In this study, we present a successful demonstration of large-scale and defect-free phosphorene synthesis on a Nickel (Ni) substrate. Additionally, we explore the substrate orientation effect, which enables the controllable synthesis of different allotropes. Specifically, we show that blue phosphorene can be grown on Ni (111) and Ni (100), while γ-Phosphorene, referred to as Navy Phosphorene, can be synthesized on Ni (110).
Our work reveals that the synthesis process involves phosphorus pentamers (P5) as a crucial precursor for phosphorene formation. Moreover, we validate the accuracy of the P-Ni and P-P potentials and demonstrate the effectiveness of the molecular dynamics approach (M.D) in simulating 2D material synthesis in the vapor phase. These results are a valuable reference for achieving large-area monolayer phosphorene synthesis.
Tree Species Identity Drives Soil Carbon and Nitrogen Stocks in Nutrient-Poor Sites
Authors: Foltran Estela Covre , Lamersdorf Norbert 
Published: August 2023

Abstract

Aims
The establishment of mixed forest stands can be seen as an option to enhance soil organic carbon stock and to protect forest ecosystems from various impacts of climate change. We examined the effect of admixture of conifers to beech forests on C stock.
Methods
We analyzed groups of European beech (Fagus sylvatica), Douglas fir (Pseudotsuga menziesii) and Norway spruce (Picea abies) stands as well as mixtures of beech with either Douglas fir or spruce under loamy versus sandy soils. We examined the stocks of C in the organic layer and upper mineral soil.
Results
The C stock of the organic layer was largely depending on tree species, whereas the C stock of the mineral soil varied among soil types. Total soil organic C stocks showed significant species identities and mixing effects were most obvious due to the high SOC stocks in the organic layer. Overall, under sandy soil conditions, conifers and mixed forests allocated 10% more SOC and N at the organic layer compared to loamy soils, whereas the SOC and N stocks under beech maintained the same proportion, independent of the site condition. The interaction between species and sites was significant only for Douglas Fir and mixed Douglas Fir/beech, indicating that the effect of species on C and N varied across sites, being significantly high at sandy soils.
Conclusion
The higher potential for carbon and N storage in mixed-species forests compared to pure stands emphasizes the capacity of mixed forest to provide valuable ecosystem services, enhancing C sequestration.
Addition of Green and Black Liquor in Kraft Pulping of Eucalyptus dunnii wood: Possible Solutions for the Problems with Kraft Pulping Caused by High Calcium Content
Authors: Vegunta Vijaya , Sevastyanova Olena , Deshpande Raghu , Lindén Pär A. , Garcia Andres , Björk Maria , Jansson Ulla , Henriksson Gunnar , Lindström Mikael E. 
Published: August 2023

Abstract
In our previous study, we demonstrated that Eucalyptus dunnii samples containing high calcium content show inferior pulping properties concerning delignification and polysaccharide degradation. This led us to investigate alternative methods for improving the pulping process of these samples. In the present work, we evaluated the effects of incorporating black and green liquors into the Eucalyptus dunnii chips before kraft pulping, aiming to enhance the pulping process and overcome the negative impact of high calcium content. The addition of both black and green liquors resulted in specific enhancements, with the green liquor having a more significant impact on the pulping process. Even wood samples with the highest calcium content demonstrated satisfactory pulping results when treated with green liquor. Delignification occurred more rapidly, and selectivity was higher for samples pre-treated with green liquor before kraft pulping. Moreover, calcium tended to follow the fiber under these conditions rather than being released into the black liquor, which may contribute to the improved pulping performance. Subsequent bleaching tests revealed that the bleachability of green liquor-treated pulp was nearly identical to that of a control pulp, while maintaining a higher viscosity. This suggests that incorporating green liquor into the pre-treatment process not only improves the pulping performance of Eucalyptus dunnii samples with high calcium content but also maintains desirable bleachability characteristics. To better understand the underlying mechanisms of these findings, we discuss the potential chemical explanations behind the observed improvements.
CYLD regulates cell ferroptosis through Hippo/YAP Signaling in prostate cancer progression
Authors: Wu Kaijie , Gu Yanan , Wu Shiqi , Fan Junjie , Meng Zeji , Liu Tianjie , Wang Qi , Xia Huayu , Gao Guoqiang , Wang Xinyang 
Published: August 2023

Abstract
Prostate cancer (PCa) is one of the most common malignancy in men. However, the molecular mechanism of its pathogenesis has not yet been elucidated. In this study, we demonstrated CYLD, a novel deubiquitinating enzyme, was downregulated in PCa tissues, and its expression was inversely correlated with pathological grade and clinical stage. Moreover, we discovered that CYLD inhibited tumor cell proliferation and promoted cell ferroptosis in PCa in vitro and in vivo, respectively. Mechanistically, we demonstrated that CYLD suppressed the ubiquitination of YAP protein, then promoted ACSL4 and TFRC mRNA transcription. Also, we demonstrated that CYLD could enhance the sensitivity of PCa tumors to ferroptosis in vivo. Furthermore, we discoverd for the first time that there was a positive correlation between CYLD expression and ACSL4 or TFRC expression in human PCa specimens. The results of this study suggested that CYLD acted as a tumor suppressor gene in PCa and promoted cell ferroptosis through Hippo/YAP signaling.
A therapeutic antibody targeting annexin-A1 inhibits cancer cell growth in vitro and in vivo.
Authors: Parris Chris , Crichton Scott , Fabian Charlene , Pepper Chris , Al-Ali Hussein , Butcher David , Dempsey Fiona 
Published: August 2023

Abstract
In this study we conducted the first investigation to assess the efficacy of a novel therapeutic antibody developed to target annexin-A1 (ANXA1). ANXA1 is an immunomodulatory protein which has been shown to be overexpressed in, and promote the development and progression of, several cancer types. In particular, high ANXA1 expression levels correlate with poorer overall survival in pancreatic and triple negative breast cancers, two cancers with considerable unmet clinical need. MDX-124 is a humanised IgG1 monoclonal antibody which specifically binds to ANXA1 disrupting its interaction with formyl peptide receptors 1 and 2 (FPR1/2). Here we show that MDX-124 significantly reduced proliferation (p < 0.0132) in a dose-dependent manner across a panel of human cancer cell lines expressing ANXA1. The anti-proliferative effect of MDX-124 is instigated by arresting cell cycle progression with cancer cells accumulating in the G1 phase of the cell cycle. Furthermore, in the 4T1-luc syngeneic mouse model of triple-negative breast cancer, MDX-124 significantly inhibited tumour growth versus vehicle control (p < 0.0001). These findings suggest ANXA1 targeted therapy is a viable and innovative approach to treat tumours which express ANXA1.
Determination of optimal mining width for coal mining under the slope by of using numerical simulation
Authors: Zhou Ze , Zhou Jinlian , Lai Long , Xu Mengtang , Xu Youlin 
Published: August 2023

Abstract
The stability of slope is critical important topic in rock mass engineering. In Panzhihua #7 Coal Mine, the coal mining is occurred under the slope, to obtain the optimal mining width, 125 numerical simulations were conducted, and the corresponding FOS was calculated. From the analysis of the numerical simulation results, it can be found that FOS decrease and then increase with increasing of filling length, meanwhile, the FOS is minimum value when the filling length is within the ranges of 10m and 20m, which is under the toe of slope. Furthermore, the minimum FOS decrease with increasing of mining width. Considered the stability of the slope and mining economy, the mining width is set to 6m, and the numerical simulation results is applied to the engineering practice. To guarantee the safety of the slope, some monitoring points were distributed on the slope, the displacement from numerical simulation and engineering practice is quite close, indicating the numerical simulation results is reliable, and the mining width is reasonable.
Primary care visits in a regional area in southern China before and during Covid pandemic
Authors: Liang Jin , Huang Guojun , Zhang Jiayi , Wang Shuang , Wang Han , Huang Qingju , Du Qingfeng , Wang Ning 
Published: August 2023

Abstract
Background Primary care visits significantly reflects the population’s health needs and service operation. In the background of Chinese health system characteristics and Covid-19 pandemic, knowledge about the quantify and disease profiles of patient visits will inform health planning and resource allocation and discipline development of general practice. This study was to describe a local primary care system, its patient visits and disease profiles of the patients before and during the Covid 19 pandemic.Methods A descriptive study was conducted based on a retrospective review of electronic health records in 2018–2021 within a County Integrated Health Organization in a rural area in southern China. Descriptive statistics and the Mann-Whitney U test were employed for data analysis.Results The primary care system consisted of hospital outpatient clinics and 19 community health centres. Community had similar quantity, but older age of patients compared to those of the hospital across the four years. Female visits were slightly higher than male visits in the first three years, but nearly doubled male visits in 2021. The age group of 40–64 had the largest proportion of total visits each year, while 0–5 group had the smallest proportion. Hypertension, respiratory infections, diabetes and general examination constituted a major part of top ten common ICD diagnoses. General Practice clinics had a miniscule part of total hospital visits.Conclusions The study provides a comprehensive profile of primary care service utilization. Community acted significantly in the service provision. The role of General Practice clinics in the hospital needs to be precisely defined.
Evaluating the utility of EUCAST RAST from positive blood culture in comparison with Vitek 2 AST in cancer patients from tertiary care cancer hospital in India.
Authors: Choudhari Sonali Choudhari , Bamnote Pooja Bamnote , Watchmaker Jerestin Watchmaker , Gawande Ruchita gawande , Farooqui Eram Farooqui 
Published: August 2023

Abstract
Background: Early initiation of targeted antibiotic therapy is essential for effective management of sepsis to reduce morbidity, mortality, cost of treatment and prevent antibiotic resistance. With the available microbiological reporting system, the turnaround time to report a positive blood culture is 48-72 hours. We aim to conduct a study to evaluate EUCAST (European Committee on Antimicrobial Susceptibility Testing) RAST (rapid antimicrobial susceptibility test) method from positive blood cultures in comparison with automated Vitek2 AST system in our cancer set up.
Method: This is a prospective study conducted over period of one year. All positively flagged blood culture bottles showing monomicrobial gram negative bacilli or gram positive cocci in gram stain were included in study. Positively flagged blood culture bottles were processed simultaneously as per EUCAST RAST and by routine methodology.
Results: The drug bug combinations as per EUCAST RAST was evaluated for 26 Escherichia coli, 17 Klebsiella pneumoniae and 7 Psuedomonas aeruginosa isolates for meropenem, imipenem, piperacillin tazobactam, ceftazidime ,amikacin, levofloxacin, ciprofloxacin, cefepime, cotrimoxazole, gentamicin and ceftriaxone. Overall Categorial Agreement is 92.13% at 6 hours. Overall VME, ME and mE was 1.11%, 3.61% and 5.61% respectively.
Conclusions: EUCAST RAST can be implemented in routine work flow to guide clinicians regarding antimicrobial choice at early stage.