bluuebunny/crossref_metadata_2025_split huggingface dataset:
A study of the relationship between vestibular loss and the risk of dementia using data from the south korea national insurance service survey from 2002 to 2019
Authors: Lim Sung Jin , Son Serhim , Chung Younghan , Kim Sang Yeop , Choi Hangseok , Choi June 
Published: February 2023

Abstract
This retrospective cross-sectional study assessed older adults (age 40–80 years) who were registered in the Korean National Health Insurance Service database from 2002 to 2019 for investigating the association between vestibular loss and the risk of dementia, the population was divided into three groups (general, vestibular loss, and hearing loss). Hazard ratio of dementia in vestibular loss and hearing loss groups was calculated using the national population data. A total of 2,347,610 individuals were identified (general: 2,145,609, vestibular loss: 60,525, hearing loss: 141,476). Mean ages were 53.29 years, 58.26 years, and 58.52 years, respectively. Dementia occurred in 127,081 (IR = 4.91 per 1,000 person-years), 7,705 (IR = 10.79 per 1,000 person-years), and 16,116 (IR = 9.63 per 1,000 person-years) patients, respectively. The vestibular loss and hearing loss groups had hazard ratios for dementia of 1.078 (95% CI, 1.053–1.103) and 1.064 (95% CI, 1.047–1.082), respectively, when compared with the general group. The current study suggests that vestibular loss increases the risk of dementia. Similar to hearing loss, vestibular loss should be considered a risk factor for dementia, and treatment such as adequate vestibular rehabilitation may reduce this risk.
In vitro transcription of self-assembling DNA nanoparticles
Authors: Oh Chang Yong , Henderson Eric 
Published: February 2023

Abstract
Nucleic acid nanoparticles are playing an increasingly important role in biomolecular diagnostics and therapeutics as well as a variety of other areas. The unique attributes of self-assembling DNA nanoparticles provide a potentially valuable addition or alternative to the lipid-based nanoparticles that are currently used to ferry nucleic acids in living systems. To explore this possibility, we have assessed the ability of self-assembling DNA nanoparticles to be constructed from complete gene cassettes that are capable of gene expression in vitro. In the current report we describe the somewhat counter-intuitive result that despite extensive crossovers (the stereochemical analogs of Holliday junctions) and variations in architecture, these DNA nanoparticles are amenable to gene expression as evidenced by T7 RNA polymerase-driven transcription of a reporter gene in vitro. These findings, coupled with the vastly malleable architecture and chemistry of self-assembling DNA nanoparticles, warrant further investigation of their utility in biomedical genetics.
Health Accreditation through the Perspectives of Auditors
Authors: Karaca Latif , Ozer Kubilay 
Published: February 2023

AbstractBackground: Along with the quick developments in technology, expectations in health care services and attention to quality and accreditation has increased. Objectives: This study aims to explore the perspectives of Health Accreditation Auditors (HAAs) who work for Turkish Health Care Quality and Accreditation Institute (TUSKA) on health care accreditation. Design: In this study, case study method was used. Sample: There are 202 auditors within TUSKA who got their HAA title before 2021 and so far, 57 HAAs have been assigned for active duty in the audits. Materials and Methods: In this study, participants were chosen among the HAAs who attended audits actively via snowball sampling and 15 of them were interviewed. Obtained data were analyzed by MAXQDA. Results: For the purpose of obtaining the perspectives of the HAAs, a list of 17 codes and with the review of these codes, 5 inclusive themes were created. These themes are institutional contribution, institutional development, necessity, financial performance and practicality. Conclusions: The HAAs clearly stated that accreditation process contributed to health care institutions positively, accelerated institutional development, proved to be necessary, affected institutions’ financial performance positively, and can be implied to any health care institution easily. On the other hand, there were some dissidence among the HAAs on the effect of accreditation on patient potential, perspectives of the managers on accreditation, health care institutions’ acceptance of accreditation standards, financial burden of the standards and the cost of applications.
The Relationship between Emotional Intelligence and Organizational Commitment among Nurses Working in Governmental Hospitals in Jordan
Authors: Baker Tasneem Abu , Shosha Ghada Abu , Al-Oweidat Islam , Nashwan Abdulqadir J. 
Published: February 2023

AbstractIntroduction: Nurses' emotions and feelings in response to their environment and their ability to manage their own emotions can have significant consequences on several aspects of their job. In Jordan, studies are still investigating whether emotional intelligence is significantly related to organizational commitment. Aim To investigate whether a significant relationship exists between emotional intelligence and organizational commitment among Jordanian nurses working in governmental hospitals in Jordan. Methods The study used a descriptive cross-sectional correlational design. A convenience sampling method was used to recruit participants working in governmental hospitals. A total of 200 nurses participated in the study. The Emotional Intelligence Scale and Organizational Commitment Scale were utilized for data collection. Results Participants had high levels of emotional intelligence and moderate levels of organizational commitment. Emotional intelligence had a significant, moderately strong, positive relationship with organizational commitment (r = 0.53,p < 0.01). Male nurses, widowed nurses, and nurses with higher postgraduate qualifications demonstrated significantly higher levels of emotional intelligence and organizational commitment than female nurses, single nurses, and nurses with undergraduate degrees (p < 0.05). Conclusion Nurses were highly emotionally intelligent and moderately committed to their organizations. Policies supporting the implementation of interventions to improve organizational commitment and maintain a high level of emotional intelligence should be developed and promoted by nurse managers and hospital administrators.
HPLC and thermal analysis, do they have a good correlation to quantify lycopene in mesoporous silica nanoparticles?
Authors: Carvalho Gabriela Corrêa , Nascimento André Luiz Carneiro Soares , Camargo Bruna Almeida Furquim , Marena Gabriel Davi , Sábio Rafael Miguel , Lourenço Felipe Rebello , Chorilli Marlus 
Published: February 2023

Abstract
Lycopene (LYC), a carotenoid extracted mainly from tomatoes has several biological properties, making its use desirable as nutraceutical and pharmaceutical active ingredient. However, LYC uses in therapy has limitations related to its solubility, making its association with nanocarriers (like mesoporous silica nanoparticle (MSN)) a viable alternative to overcome its drawbacks. To know the exact amount of drug incorporated the incorporation final product needs to be analysed by techniques, such as HPLC and thermal analysis. So, this study objective was to validate an HPLC method for LYC quantification and evaluate its applicability in determine LYC encapsulation in MSNs, additionally, as a way of checking and comparing the thermal analysis technique was used. For the applicability, two types of MSNs were evaluated and several conditions were tested. The developed method was properly validated and regarding its applicability, the vast majority of the conditions evaluated proved to be compatible with the method used. As for the comparison, thermal analysis and HPLC analysis showed a good correlation. In conclusion, the HPLC method has been properly validated and proved to be adequate for LYC quantification from MSNs. Additionally, for the greater results reliability, the association between the two techniques used here proved to be desirable.
NT-proBNP Level as a Substitute for Myocardial Perfusion Scan in Preoperative Cardiovascular Risk Assessment in Noncardiac Surgery
Authors: Esmati Saeede , Tavoosi Anahita , Mehraban Saghar , Far Vahideh Laleh , Mehrakizadeh Ali , Shahi Shayan , Larti Farnoosh 
Published: February 2023

Abstract
Background
Preoperative cardiovascular risk assessment is one of the main principles before noncardiac surgeries. Cardiac stress imaging, such as myocardial perfusion scan (MPI), is one of the proposed cardiac risk evaluation methods according to the latest guidelines. Yet, its efficacy, along with the cost-effectiveness of the method, has been questioned in previous studies. Our study aims to evaluate the utility of NT-proBNP level measurement in predicting postoperative cardiovascular complications in candidates who have undergone an MPI scan before surgery and compare the results.
Methods
A cohort of 78 patients with an RCRI score of one or more who were scheduled for high to moderate-risk noncardiac surgeries and met the criteria to undergo an MPI scan for risk assessment were included in the study. All patients underwent an MPI scan one week before surgery. Their preoperative NT-proBNP, troponin levels, and ECGs were obtained one day before surgery. The predictive efficacy of NT-proBNP levels and MPI scans were compared.
Results
Seventy-eight patients underwent surgery, of which three patients exhibited a rise in troponin level, six showed ECG changes, and pulmonary edema was detected in one patient three days after surgery. There was no mortality. The sensitivity and specificity of the MPI scan for predicting postoperative adverse cardiovascular outcomes were 100% and 66%, respectively. MPI scan also had a positive predictive value (PPV) of 20% and a negative predictive value (NPV) of 100%. A 332.5 pg/ml cut-off value for NT-proBNP level yielded a sensitivity of 100%, specificity of 79.2%, PPV of 40%, and NPV of 100%.
Conclusions
This study reveals the incremental prognostic value of NT-proBNP level measurement in preoperative cardiac risk evaluation compared to an MPI scan. Given the low feasibility, high costs, and disappointing predictive value of MPI scans, preoperative NT-proBNP level assessment can be substituted. This method can assist anesthesiologists and surgeons with detecting at-risk patients resulting in taking proper measures to reduce morbidity and mortality in the preoperative period.
Subaqueous 3D stem cell spheroid levitation culture using anti-gravity bioreactor based on sound wave superposition
Authors: Park Jung Hwan , Lee Ju-Ro , Park Sungkwon , Kim Yu-Jin , Yoon Jeong-Kee , Joung Yoon Ki , Lee Tae Il , Bhang Suk Ho 
Published: February 2023

Abstract
Background
Recently, various studies have revealed that 3D cell spheroids have several advantages over 2D cells in stem cell culture. However, conventional 3D spheroid culture methods have some disadvantages and limitations such as time required for spheroid formation and complexity of the experimental process. Here, we used acoustic levitation as cell culture platform to overcome the limitation of conventional 3D culture methods.
Methods
In our anti-gravity bioreactor, continuous standing sonic waves created pressure field for 3D culture of human mesenchymal stem cells (hMSCs). hMSCs were trapped and aggerated in pressure field and consequently formed spheroids. We injected hMSC spheroids fabricated by anti-gravity bioreactor into the mouse hindlimb ischemia model.
Results
The acoustic levitation in anti-gravity bioreactor made spheroids faster and more compact compared to the conventional hanging drop method, which resulted in the upregulation of angiogenic paracrine factors of hMSCs. Injected hMSCs spheroids cultured in anti-gravity bioreactor exhibited improved therapeutic efficacy for mouse hindlimb ischemia model compared to spheroids formed by the conventional hanging drop method.
Conclusion
Our stem cell culture system using acoustic levitation will be proposed as a new platform for the future 3D cell culture system.
Clinical outcomes of arch expansion with Invisalign: a systematic review
Authors: MA SONGYANG MA , WANG YUNJI WANG 
Published: February 2023

Abstract
Objective: To assess the scientific evidence related to the clinical outcomes of Invisalign therapy in controlling orthodontic tooth movement.
Materials and Methods: An electronic search was conducted in Pubmed，Cochrane Library，Web of Science，Embase，and Scopus from November 2015 to November 2022 to identify all articles. Methodological shortcomings were highlighted and the quality of the studies was ranked using the Cochrane Tool for Risk of Bias Assessment.
Results: Three prospective, and twelve retrospective studies were included. Besides, the risk of bias was moderate for thirteen studies and unclear for the others. There’s statistically significant difference between the prettreatment and posttreatment arches. The average expansion was significantly different from that predicted for each type of tooth in both the maxilla and mandible. Furthermore, the efficiency decreased from the anterior area to the posterior area in the upper arch.
Conclusion: Despite the fact that arch expansion with Invisalign® is not ideally predictable, clear treatment is considerable option to resolve dentition crowding. The efficacy of expansion performs greatest in the premolar area. More researches focusing on the treatment outcome with different materials of aligners should be conducted in the future. Overcorrection should be considered when planning arch expansion with Invisalign. In maxilla, the expansion rate decreases from anterior to posterior. In maxilla, presetting sufficient buccal root torque of posterior teeth may result in better efficiency of expansion.
Screening for potential biological markers of chronic kidney disease based on WGCNA and machine learning
Authors: Zhang Chengyu , Feng Sujuan , Li Han , Wang Shixiang 
Published: February 2023

Abstract
Background: Chronic kidney disease (CKD) is defined as persistent urinary tract abnormalities, structural abnormalities, or impaired excretory function of the kidneys, which is the 16th leading cause of years of life lost worldwide and places an enormous burden on medical care. However, the mechanisms for the progression of CKD are still poorly understood. Therefore, the aim of this study is to identify the genes responsible for CKD and to establish a genetic diagnosis model.
Results: By combining the differentially expressed genes with the Weighted correlation network analysis, a total of 264 differentially expressed genes, most associated with CKD were identified. According to the results of GO enrichment analysis, we confirmed the molecular functions were most closely related to haptoglobin binding and oxygen carrier. In the biological process, the term most related to oxygen transport, and in cellular components was hemoglobin complexes. KEGG enrichment analysis showed that these DEGs were related to pancreatic secretion, protein digestion, and absorption. Subsequently, ZCCHC7, ZNF396, and EIF4E3 were identified as three potential biological markers in the kidney of patients with CKD by using Least absolute shrinkage and selection operator (LASSO) regression and machine learning algorithms. Gene Set Enrichment Analysis (GSEA) furtherly demonstrated that three genes respectively involved in lipid (ZCCHC7), glucose (ZNF396), and metabolism (EIF4E3).A diagnostic model was also constructed based on these three genes, and the ROC curve showed that the 3-gene diagnostic model has a good fit. Finally, the three potential biological markers of
CKD and their model were validated by using GSE175759, and the results further indicated the diagnostic value of these three potential biological markers.
Conclusions: We successfully identified and validated that ZCCHC7, ZNF396, and EIF4E3 are potential biological markers in CKD patients, which may influence the progression of CKD via the metabolism of fat, sugar, and steroid hormones. Our findings offered a potential diagnostic biomarker for CKD.
“Herbal medicine use during pregnancy and child birth may prevent its related complications”: Perceptions of women living in Lilongwe rural, Malawi – A Qualitative Study.
Authors: Makombe Dziwenji , Thombozi Enalla , Chilemba Winnie , Mboma Alexander , Banda Kondwani Joseph , Mwakilama Elias 
Published: February 2023

Abstract
Background: Use of herbal medicine during pregnancy and labour is often associated with obstetric complications including uterine rupture and fetal distress, but little is known about its advantages. Particularly, in-depth information on the perceptions of rural women in Malawi about the use of herbal medicine during pregnancy and labour is underreported. Knoweldge of women’s views and perceptions on use of herbal medicine would help determine potential inclusion of the suitable herbal medicine in women and reproductive health services.
Aims: To explore the perceptions of women on potential use of herbal medicine during pregnancy and labour in rural Malawi.
Methods: We employed a crossesctional survey on study participants (women), purposively identified (parity≥2) from four villages (Kagona, Champsinja, Mthupi and Manja) of Traditional Authority Malili, in Lilongwe rural district, Malawi. Qualtitative data was collected through four Focus Groups of 6-8 women in each group that were conducted in each village. Data analysis was performed using content analysis inductive approach.
Results: A total of 28 women of reproductive age 20 and above; 20-24 (32.14%), married (75%), average of 3 deliveries (57.14%), primary school education (75.0%), and Christians (92.86%) were recruited and interviewed. Two main themes emerged from the narratives: (1) herbal medicine is beneficial: (i) hastens labour, (ii) prevents pregnancy complications and illnesses, and (2) herbal medicine is risky: (i) leads to caesarean delivery, (ii) leads to ruptured uterus and death, (iv) leads to birth asphyxia, and still births.
Conclusion: Perceptions among rural women on use of herbal medicine during pregnancy and labour are deep routed on cultural and personal experiences. Health education on health related dangers associated with use of herbal medicine among rural women in Malawi can help prevent associated pregnancy and labour complications and improve maternity care. Further research is warranted to explore accessibility and community pathway systems for herbal medicine use during pregnancy and labour among the pregnant women.
Effect of mulching on soil properties, microbial diversity and activities, and crop yield
Authors: Jacquiod Samuel , Bouchard Elodie , Roure Frédéric , Cheviron Nathalie , Mougin Christian , Coffin Arnaud , Blouin Manuel , Martin-Laurent Fabrice 
Published: February 2023

Abstract
Aims - Plastic films are used to mulch soils to control weeds, especially in organic farming. Their application leaves persistent plastic fragments in soils, with poorly understood environmental and health consequences. Plant fiber textiles (bio-canvas) are promising alternatives since they are more persistent than straw mulching and are entirely biodegradable. Hemp fibers are particularly interesting materials due to their renowned resistance, allelopathic and trophic properties for soil life. However, their effects on soil microbiota and yield remain unclear. Methods - In a greenhouse experiment, we assessed the effect of soil mulching (bare soil control, plastic mulch, hemp straw mulching, hemp-canvas) on lettuce growth, soil climatic conditions, enzymatic activities and microbial communities (bacteria and fungi). Our experiment allowed to distinguish effects associated to mulching, being i) the homogeneity of soil covering (plastic mulch and hemp canvas) or not (control, hemp mulch), ii) the biodegradability (hemp mulch, hemp canvas) or not (control, plastic mulch), and iii) their interaction. Results - An interaction occurred between cover homogeneity and biodegradability when using the hemp canvas, leading to higher soil relative water content, stable soil temperature, higher laccase and arylamidase activities, and different soil microbial community structures and fungal diversity, with comparable lettuce yields to that obtained with plastic mulch. Plastic cover induced higher soil temperatures, lower enzymatic activities, and different soil microbial community structures. Conclusions - We conclude that hemp canvas secures lettuce yields, but through different mechanisms compared to plastic mulch, notably via a biostimulating effect on soil microbial diversity and functioning.
Plasmodium ARK2-EB1 axis drives the unconventional spindle dynamics, scaffold formation and chromosome segregation of sexual transmission stages
Authors: Zeeshan Mohammad , Rea Edward , Abel Steven , Vukusic Kruno , Markus Robert , Brady Declan , Eze Anthonius , Rashpa Ravish , Balestra Aurélia , Bottrill Andrew , Brochet Mathieu , Guttery David , Tolić Iva , Holder Anthony , Roch Karine Le , Tromer Eelco , Tewari Rita 
Published: February 2023

Abstract
Mechanisms of cell division are remarkably diverse, suggesting the underlying molecular networks among eukaryotes differ extensively. The Aurora family of kinases orchestrates the process of chromosome segregation and cytokinesis during cell division through precise spatiotemporal regulation of their catalytic activities by distinct scaffolds. Plasmodium spp., the causative agents of malaria, are unicellular eukaryotes that have three divergent aurora-related kinases (ARKs) and lack most canonical scaffolds/activators. The parasite uses unconventional modes of chromosome segregation during endomitosis and meiosis in sexual transmission stages within mosquito host. This includes a rapid threefold genome replication from 1N to 8N with successive cycles of closed mitosis, spindle formation and chromosome segregation within eight minutes (termed male gametogony). Kinome studies had previously suggested likely essential functions for all three Plasmodium ARKs during asexual mitotic cycles; however, little is known about their location, function, or their scaffolding molecules during unconventional sexual proliferative stages. Using a combination of super-resolution microscopy, mass spectrometry, omics and live-cell fluorescence imaging, we set out to investigate the contribution of the atypical Aurora paralog ARK2 to proliferative sexual stages using rodent malaria model Plasmodium berghei. We find that ARK2 primarily localises to the spindle apparatus associated with kinetochores during both mitosis and meiosis. Interactomics and co-localisation studies reveal a unique ARK2 scaffold at the spindle including the microtubule plus end-binding protein EB1 and lacking some other conserved molecules. Gene function studies indicate complementary functions of ARK2 and EB1 in driving endomitotic divisions and thereby parasite transmission. Our discovery of a novel Aurora spindle scaffold underlines the emerging flexibility of molecular networks to rewire and drive unconventional mechanisms of chromosome segregation in the malaria parasite Plasmodium.