bluuebunny/crossref_metadata_2025_split huggingface dataset:
Density and temperature controlled fluid extraction in a bacterial biofilm is determined by poly-γ-glutamic acid production
Authors: Morris Ryan J. , Stevenson David , Sukhodub Tetyana , Stanley-Wall Nicola R. , MacPhee Cait E. 
Published: December 2022

AbstractA hallmark of microbial biofilms is the self-production of an extracellular molecular matrix that encases the resident cells. The matrix provides protection from the environment, while spatial heterogeneity of gene expression influences the structural morphology and colony spreading dynamics. Bacillus subtilis is a model bacterial system used to uncover the regulatory pathways and key building blocks required for biofilm growth and development. In this work, we report on the emergence of a highly active population of bacteria during the early stages of biofilm formation, facilitated by the extraction of fluid from the underlying agar substrate. We trace the origin of this fluid extraction to the production of poly-γ-glutamic acid (PGA). The flagella-dependent activity develops behind a moving front of fluid that propagates from the boundary of the biofilm towards the interior. The extent of fluid proliferation is controlled by the presence of extracellular polysaccharides (EPS). We also find that PGA production is positively correlated with higher temperatures, resulting in high-temperature mature biofilm morphologies that are distinct from the rugose colony biofilm architecture typically associated with B. subtilis. Although previous reports have suggested that PGA production does not play a major role in biofilm morphology in the undomesticated isolate NCIB 3610, our results suggest that this strain produces distinct biofilm matrices in response to environmental conditions.
Anomalous superconducting proximity effect of planar Pb–RhPb2 heterojunctions in the clean limit
Authors: Wang Rui-Feng , Xiong Yan-Ling , Zhu Qun , Ren Ming-Qiang , Yan Hang , Song Can-Li , Ma Xu-Cun , Xue Qi-Kun 
Published: December 2022

AbstractInterest in superconducting proximity effect has been revived by the exploitation of Andreev states and by the possible emergence of Majorana bound states at the interface. Spectroscopy of these states has been so far restricted to just a handful of superconductor-metal systems in the diffusion regime, whereas reports in otherwise clean superconductor-superconductor heterojunctions are scarce. Here, we realize molecular beam epitaxy growth of atomically sharp planar heterojunctions between Pb and a topological superconductor candidate RhPb2 that allows us to spectroscopically image the proximity effect in the clean limit. The measured energy spectra of RhPb2 vary with the spatial separation from proximal Pb, and exhibit unusual modifications in the pairing gap structure and size that extend over a distance far beyond the coherence length. This anomalously long-range proximity (LRP) effect breaks the rotational symmetry of Cooper pair potential in real space and largely deforms the Abrikosov vortex cores. Our work opens promising avenues for fundamental studies of the Andreev physics and extraordinary states in clean superconducting heterojunctions.
Multivalent viral particles elicit safe and efficient immunoprotection against Nipah Hendra and Ebola viruses
Authors: Ithinji Duncan G. , Buchholz David W. , Ezzatpour Shahrzad , Monreal I. Abrrey , Cong Yu , Sahler Julie , Bangar Amandip Singh , Imbiakha Brian , Upadhye Viraj , Liang Janie , Ma Andrew , Bradel-Tretheway Birgit , Kaza Benjamin , Yeo Yao Yu , Choi Eun Jin , Johnston Gunner P. , Huzella Louis , Kollins Erin , Dixit Saurabh , Yu Shuiqing , Postnikova Elena , Ortega Victoria , August Avery , Holbrook Michael R. , Aguilar Hector C. 
Published: December 2022

AbstractExperimental vaccines for the deadly zoonotic Nipah (NiV), Hendra (HeV), and Ebola (EBOV) viruses have focused on targeting individual viruses, although their geographical and bat reservoir host overlaps warrant creation of multivalent vaccines. Here we explored whether replication-incompetent pseudotyped vesicular stomatitis virus (VSV) virions or NiV-based virus-like particles (VLPs) were suitable multivalent vaccine platforms by co-incorporating multiple surface glycoproteins from NiV, HeV, and EBOV onto these virions. We then enhanced the vaccines’ thermotolerance using carbohydrates to enhance applicability in global regions that lack cold-chain infrastructure. Excitingly, in a Syrian hamster model of disease, the VSV multivalent vaccine elicited safe, strong, and protective neutralizing antibody responses against challenge with NiV, HeV, or EBOV. Our study provides proof-of-principle evidence that replication-incompetent multivalent viral particle vaccines are sufficient to provide protection against multiple zoonotic deadly viruses with high pandemic potential.
Extracellular arginine is required but the arginine transporter CAT3 (Slc7a3) is dispensable for mouse normal and malignant hematopoiesis
Authors: Yan Yuhan , Chen Chao , Li Zhiguo , Zhang Jing , Park Narin , Qu Cheng-Kui 
Published: December 2022

AbstractAmino acid-mediated metabolism is one of the key catabolic and anabolic processes involved in diverse cellular functions. However, the role of the semi-essential amino acid arginine in normal and malignant hematopoietic cell development is poorly understood. Here we report that a continuous supply of exogenous arginine is required for the maintenance/function of normal hematopoietic stem cells (HSCs). Surprisingly, knockout of Slc7a3 (CAT3), a major L-arginine transporter, does not affect HSCs in steady-state or under stress. Although Slc7a3 is highly expressed in naïve and activated CD8 T cells, neither T cell development nor activation/proliferation is impacted by Slc7a3 depletion. Furthermore, the Slc7a3 deletion does not attenuate leukemia development driven by Pten loss or the oncogenic Ptpn11E76K mutation. Arginine uptake assays reveal that L-arginine uptake is not disrupted in Slc7a3 knockout cells. These data suggest that extracellular arginine is critically important for HSCs, but CAT3 is dispensable for normal hematopoiesis and leukemogenesis.
Fake news reminders and veracity labels differentially benefit memory and belief accuracy for news headlines
Authors: Kemp Paige L. , Loaiza Vanessa M. , Wahlheim Christopher N. 
Published: December 2022

AbstractFake news exposure can negatively affect memory and beliefs, thus sparking debate about whether to repeat misinformation during corrections. The once-prevailing view was that repeating misinformation increases its believability and should thus be avoided. However, misinformation reminders have more recently been shown to enhance memory and belief accuracy. We replicated such reminder benefits in two experiments using news headlines and compared those benefits against the effects of veracity labeling. Specifically, we examined the effects of labeling real news corrections to enhance conflict salience (Experiment 1) and labeling fake news on its debut to encourage intentional forgetting (Experiment 2). Participants first viewed real and fake news headlines with some fake news labeled as false. Participants then saw labeled and unlabeled real news corrections; labeled corrections appeared alone or after fake news reminders. Reminders promoted the best memory and belief accuracy, whereas veracity labels had selective effects. Correction labels led to intermediate memory and belief accuracy, whereas fake news labels improved accuracy for beliefs more than memory. The extent that real and fake news details were recalled together correlated with overall memory and belief differences across conditions, implicating a critical role for integrative encoding that was promoted most by fake news reminders.
Inheritance of 2,4-dichlorophenoxyacetic acid (2,4-D) resistance in Amaranthus palmeri
Authors: Shyam Chandrima , Peterson Dallas E. , Jhala Amit J. , Jugulam Mithila 
Published: December 2022

AbstractIn this study, the inheritance of 2,4-D resistance in a multiple herbicide-resistant Palmer amaranth (KCTR) was investigated. Direct and reciprocal crosses were performed using 2,4-D-resistant KCTR and susceptible KSS plants to generate F1 progenies. 2,4-D dose–response assays were conducted to evaluate the response of progenies from each F1 family along with KCTR and KSS plants in controlled environmental growth chambers. Additionally, 2,4-D-resistant male and female plants from each of the F1 families were used in pairwise crosses to generate pseudo-F2 families. Segregation (resistance or susceptibility) of progenies from the F2 families in response to a discriminatory rate of 2,4-D (i.e., 560 g ae ha−1) was evaluated. Dose–response analysis of F1 progenies derived from direct and reciprocal crosses suggested that the 2,4-D resistance in KCTR is a nuclear trait. Chi-square analyses of F2 segregation data implied that 2,4-D resistance in KCTR is controlled by multiple gene(s). Overall, our data suggest that the 2,4-D resistance in KCTR Palmer amaranth is a nuclear inherited trait controlled by multiple genes. Such resistance can spread both via pollen or seed-mediated gene flow. In future, efforts will be directed towards identifying genes mediating 2,4-D resistance in KCTR population.
Monitoring SARS-CoV-2 variant transitions using differences in diagnostic cycle threshold values of target genes
Authors: Bordoy Antoni E. , Saludes Verónica , Panisello Yagüe David , Clarà Gemma , Soler Laia , Paris de León Alexia , Casañ Cristina , Blanco-Suárez Ana , Guerrero-Murillo Mercedes , Rodríguez-Ponga Beatriz , Noguera-Julian Marc , Català-Moll Francesc , Pey Irina , Armengol Maria Pilar , Casadellà Maria , Parera Mariona , Pluvinet Raquel , Sumoy Lauro , Clotet Bonaventura , Giménez Montserrat , Martró Elisa , Cardona Pere-Joan , Blanco Ignacio 
Published: December 2022

AbstractMonitoring the emergence of new SARS-CoV-2 variants is important to detect potential risks of increased transmission or disease severity. We investigated the identification of SARS-CoV-2 variants from real-time reverse transcriptase polymerase chain reaction (RT-PCR) routine diagnostics data. Cycle threshold (Ct) values of positive samples were collected from April 2021 to January 2022 in the Northern Metropolitan Area of Barcelona (n = 15,254). Viral lineage identification from whole genome sequencing (WGS) was available for 4618 (30.3%) of these samples. Pairwise differences in the Ct values between gene targets (ΔCt) were analyzed for variants of concern or interest circulating in our area. A specific delay in the Ct of the N-gene compared to the RdRp-gene (ΔCtNR) was observed for Alpha, Delta, Eta and Omicron. Temporal differences in ΔCtNR correlated with the dynamics of viral replacement of Alpha by Delta and of Delta by Omicron according to WGS results. Using ΔCtNR, prediction of new variants of concern at early stages of circulation was achieved with high sensitivity and specificity (91.1% and 97.8% for Delta; 98.5% and 90.8% for Omicron). Thus, tracking population-wide trends in ΔCt values obtained from routine diagnostics testing in combination with WGS could be useful for real-time management and response to local epidemics.
Label-free testing strategy to evaluate packed red blood cell quality before transfusion to leukemia patients
Authors: Dybas Jakub , Wajda Aleksandra , Alcicek Fatih Celal , Kaczmarska Magdalena , Bulat Katarzyna , Szczesny-Malysiak Ewa , Martyna Agnieszka , Perez-Guaita David , Sacha Tomasz , Marzec Katarzyna M. 
Published: December 2022

AbstractPatients worldwide require therapeutic transfusions of packed red blood cells (pRBCs), which is applied to the high-risk patients who need periodic transfusions due to leukemia, lymphoma, myeloma and other blood diseases or disorders. Contrary to the general hospital population where the transfusions are carried out mainly for healthy trauma patients, in case of high-risk patients the proper quality of pRBCs is crucial. This leads to an increased demand for efficient technology providing information on the pRBCs alterations deteriorating their quality. Here we present the design of an innovative, label-free, noninvasive, rapid Raman spectroscopy-based method for pRBCs quality evaluation, starting with the description of sample measurement and data analysis, through correlation of spectroscopic results with reference techniques' outcomes, and finishing with methodology verification and its application in clinical conditions. We have shown that Raman spectra collected from the pRBCs supernatant mixture with a proper chemometric analysis conducted for a minimum one ratio of integral intensities of the chosen Raman marker bands within the spectrum allow evaluation of the pRBC quality in a rapid, noninvasive, and free-label manner, without unsealing the pRBCs bag. Subsequently, spectroscopic data were compared with predefined reference values, either from pRBCs expiration or those defining the pRBCs quality, allowing to assess their utility for transfusion to patients with acute myeloid leukemia (AML) and lymphoblastic leukemia (ALL).
Mexican validation of the International Affective Digitized Sounds second edition (IADS-2) and additional sounds
Authors: Naal-Ruiz Norberto E. , Alonso-Valerdi Luz M. , Ibarra-Zarate David I. , Serrano-Cena Alba , Navas-Reascos Gustavo 
Published: December 2022

AbstractAffective stimuli have been extensively used in emotion research for a better understanding of emotion regulation. Sound ratings, specifically non-verbal sounds, are biased by demographic indicators such as sex and nationality. Therefore, it is crucial to characterize sounds prior to their use in emotion research. This study aims to validate the IADS-2 database and additional sounds in a sample from the Mexican population. Three hundred twenty-nine participants born and raised in Mexico remotely listened to 174 sounds in monophonic format. They rated sounds according to the valence-arousal-dominance model using the Self-Assessment Manikin test. Results positively correlated to those of previous studies. Sex differences were observed only in dominance between female and male groups, contrary to the results from Portuguese, American and Japanese validations. Geographic region analysis demonstrated differences in arousal, indicating the need for additional research on occident and south regions. Furthermore, when conducting affective research, headphones and audio quality should be considered, primarily to reduce variability due to audio-related aspects, and to avoid changes in emotional experience. Finally, this study supports the feasibility of remote affective sound experiments over the internet as reported in previous research.
The efficacy of ocular surface assessment approaches in evaluating dry eye treatment with artificial tears
Authors: Szczesna-Iskander Dorota H. , Muzyka-Wozniak Maria , Llorens Quintana Clara 
Published: December 2022

AbstractThis study evaluates the effectiveness of objective techniques in assessing dry eye disease (DED) treatment compared with the subjective assessment commonly used in clinical practice. Thirty subjects were recruited for two visits separated by 28(± 3) days of treatment with artificial tears. A buttery of common subjective assessment methods were accompanied by a set of objective techniques including measurement of noninvasive tear film break-up time (NIBUT), lipid layer thickness (LLT), and quantitative evaluation of tear film surface quality and dynamics (TFD). Additionally, meibography was performed. Two commercially available videokeratoscopes and a prototype of a lateral shearing interferometer were used for the measurements. Both subjective and objective techniques showed a positive effect of artificial tears in DED treatment. Statistically significant improvements were observed in subjective symptoms (from P < 0.001 for Ocular Surface Disease Index, OSDI to p = 0.019 for tearing), conjunctival redness (P = 0.022), ocular staining (P = 0.012), fluorescein tear film break-up time (P = 0.015), NIBUT (P = 0.037), LLT (P < 0.001), and TFD (P = 0.048). In general, weak or statistically insignificant correlations were observed between subjective and objective assessment methods. The apparent lack of correlation between these methods might indicate the complementary character of objective techniques that likely assess other characteristics of ocular surface health than those assessed subjectively.
Fig latex inhibits the growth of pathogenic bacteria invading human diabetic wounds and accelerates wound closure in diabetic mice
Authors: Salah Mohamed , Badr Gamal , Hetta Helal F. , Khalifa Walaa A. , Shoreit Ahmed A. 
Published: December 2022

AbstractImpaired wound healing is one of the most critical complications associated with diabetes mellitus. Infections and foot ulcers are major causes of morbidity for diabetic patients. The current treatment of diabetic foot ulcers, commonly used antibiotics, is associated with the development of bacterial resistance. Hence, novel and more effective natural therapeutic antibacterial agents are urgently needed and should be developed against the pathogenic bacteria inhabiting diabetic wounds. Therefore, the current study aimed to investigate the impact of fig latex on pathogenic bacteria and its ability to promote the healing process of diabetic wounds. The pathogenic bacteria were isolated from patients with diabetic foot ulcers admitted to Assiut University Hospital. Fig latex was collected from trees in the Assiut region, and its chemical composition was analyzed using GC‒MS. The antibacterial efficacy of fig latex was assessed on the isolated bacteria. An in vivo study to investigate the effect of fig latex on diabetic wound healing was performed using three mouse groups: nondiabetic control mice, diabetic mice and diabetic mice treated with fig latex. The influence of fig latex on the expression levels of β-defensin-1, PECAM-1, CCL2 and ZO-1 and collagen formation was investigated. The GC‒MS analysis demonstrated the presence of triterpenoids, comprising more than 90% of the total latex content. Furthermore, using a streptozotocin-induced diabetic mouse model, topical treatment of diabetic wound tissues with fig latex was shown to accelerate and improve wound closure by increasing the expression levels of β-defensin-1, collagen, and PECAM-1 compared to untreated diabetic wounds. Additionally, fig latex decreased the expression levels of ZO-1 and CCL2.
Improvement of a coastal vulnerability index and its application along the Calabria Coastline, Italy
Authors: Pantusa Daniela , D’Alessandro Felice , Frega Ferdinando , Francone Antonio , Tomasicchio Giuseppe Roberto 
Published: December 2022

AbstractThe present paper further develops a coastal vulnerability index formulation (CVI) previously proposed by the authors by integrating a new variable and redefining three variables to improve the suitability of the index for low-lying coasts. Eleven variables are divided into three typological groups: geological, hydro-physical process and vegetation. The geological variables are: geomorphology, shoreline erosion/accretion rates, coastal slope, emerged beach width, and dune. The hydro-physical process group includes: river discharge, sea-level change, mean significant wave height and mean tide range. The vegetation variables are: vegetation behind the back-beach and coverage of Posidonia oceanica. The index was applied to a stretch of the Ionian coast in the province of Crotone in the Calabria region (Southern Italy), and a vulnerability map was produced. A geography information system (GIS) platform was used to better process the data. For the case study area, the most influential variables are shoreline erosion/accretion rates, coastal slope, emerged beach width, dune, vegetation behind the back-beach, and coverage of Posidonia oceanica. The most vulnerable transects are those near urban areas characterized by the absence of dunes and vegetation. Statistical and sensitivity analyses were performed, and the proposed CVI was compared with the previous formulation proposed by the authors and with two other CVI methods present in the literature.
Three-dimensional analysis of modeled facial aging and sexual dimorphism from juvenile to elderly age
Authors: Velemínská Jana , Jaklová Lenka Kožejová , Kočandrlová Karolina , Hoffmannová Eva , Koudelová Jana , Suchá Barbora , Dupej Ján 
Published: December 2022

AbstractA detailed understanding of craniofacial ontogenetic development is important in a variety of scientific disciplines dealing with facial reconstruction, forensic identification, ageing prediction, and monitoring of pathological growth, including the effect of therapy. The main goals of this study were (1) the construction of the facial aging model using local polynomial regression fitting separately for both sexes, (2) evaluation of the aging effect not only on facial form as a whole but also on dimensions important for clinical practice, and (3) monitoring of the development of shape facial sexual dimorphism. Our study was based on the form and shape analysis of three-dimensional facial surface models of 456 individuals aged 14–83 years. The facial models were obtained using a structured light-based optical scanner and divided (for some analyses) into four age categories (juveniles, young adults, middle adults, and elderly adults). The methodology was based on geometric and classic morphometrics including multivariate statistics. Aging in both sexes shared common traits such as more pronounced facial roundness reducing facial convexity, sagging soft tissue, smaller visible areas of the eyes, greater nose, and thinner lips. In contrast to female faces, male faces increase in size until almost 30 years of age. After the age of 70, male facial size not only stagnates, like in females, but actually decreases slightly. Sexual dimorphic traits tended to diminish in the frontal and orbitonasal areas and increase in the gonial area.
Rate of force development relationships to muscle architecture and contractile behavior in the human vastus lateralis
Authors: Werkhausen Amelie , Gløersen Øyvind , Nordez Antoine , Paulsen Gøran , Bojsen-Møller Jens , Seynnes Olivier R. 
Published: December 2022

AbstractIn this study, we tested the hypotheses that (i) rate of force development (RFD) is correlated to muscle architecture and dynamics and that (ii) force–length–velocity properties limit knee extensor RFD. Twenty-one healthy participants were tested using ultrasonography and dynamometry. Vastus lateralis optimal fascicle length, fascicle velocity, change in pennation angle, change in muscle length, architectural gear ratio, and force were measured during rapid fixed-end contractions at 60° knee angle to determine RFD. Isokinetic and isometric tests were used to estimate individual force–length–velocity properties, to evaluate force production relative to maximal potential. Correlation analyses were performed between force and muscle parameters for the first three 50 ms intervals. RFD was not related to optimal fascicle length for any measured time interval, but RFD was positively correlated to fascicle shortening velocity during all intervals (r = 0.49–0.69). Except for the first interval, RFD was also related to trigonometry-based changes in muscle length and pennation angle (r = 0.45–0.63) but not to architectural gear ratio. Participants reached their individual vastus lateralis force–length–velocity potential (i.e. their theoretical maximal force at a given length and shortening velocity) after 62 ± 24 ms. Our results confirm the theoretical importance of fascicle shortening velocity and force–length–velocity properties for rapid force production and suggest a role of fascicle rotation.