bluuebunny/crossref_metadata_2025_split huggingface dataset:
Green Building Literacy and University Students' Housing Choice in Ghana
Authors: Donkor-Hyiaman Kenneth Appiah , Gyamfi-Yeboah Frank , Tudzi Eric Paul , Bugri John Tia 
Published: March 2023

Abstract
Green buildings are part of the global strategy to achieve sustainable development. Efforts towards this goal are however hampered by the lack of awareness and high illiteracy about green building. Focusing on the youth, this paper measures the level of green building literacy among Ghanaian university students, how it differs according to their demographics, and its relationship with their housing choice. Following a comprehensive literature review, we used a structured questionnaire survey to gather data from 763 university students. After statistical tests, the study analysed the dataset principally using mean scoring, Chi-square test and regression techniques. The results suggest that most university students have no or basic knowledge in green buildings. This low level of green building literacy is attributable in part to their demographic features including age, gender, level of education, level of study, employment status, income level of respondents, and whether the person had previously lived in a house with green features. Further analyses show that male student and built environment students have more knowledge of green buildings and are more likely to have lived in or are currently living in a building with green features, and also more likely to choose a house with green features in the future. Policy-wise, the study shows that educating the youth about green buildings could alter their housing outcomes and preferences in favour of sustainable ones and hence, contribute to achieving Sustainable Development Goal 11 – Sustainable Cities and Communities.
Bioinformatics and systematic biology methods to determine the pathogenic link between COVID-19 and atrial fibrillation
Authors: Li Jinglei , Hou Wei 
Published: March 2023

Abstract
The coronavirus disease 2019 (COVID-19) epidemic has not been effectively controlled thus far. Atrial fibrillation (AF) is a risk factor for COVID-19, and if not controlled in a timely manner, it will cause a catastrophic situation. However, the molecular mechanism of AF and COVID-19 is not well understood. We performed transcriptome analysis to capture common signaling pathways and molecular markers of AF and COVID-19, which will help in understanding the link between COVID-19 and AF. Three AF datasets (GSE41177, GSE31821, GSE79768) and one COVID-19 dataset (GSE147507) from the Gene Expression Omnibus (GEO) database were used in this study. Differential expression analysis of the datasets identified differential genes common to both diseases. To explore the biological mechanisms of the differential genes, gene ontology (GO) and kyoto encyclopedia of genes and genomes (KEGG), protein-protein interaction (PPI), receiver operating characteristic curve (ROC), miRNA- transcriptionfactor (TF)-mRNA network, drug candidate prediction, molecular docking, and gene-disease association analyses were performed. We finally found 63 DEGs common to COVID-19 and AF. GO/KEGG allowed the common biological functions and signaling pathways of both diseases to be understood. Based on the PPI network, 5 hub genes (FCGR3B, IL1B, CXCR4, CSF2RB, and SELL) were identified, with CXCR4 as the most diagnostic and therapeutic target gene. Based on CXCR4, a miRNA-TF-mRNA network was constructed. Five potential therapeutic agents closely related to CXCR4 were identified, and their reliability was verified by molecular docking. Finally, possible associated diseases were analyzed. Taken together, our findings will help scholars understand the potential biological mechanisms between COVID-19 and AF. The development of precise therapeutic agents targeting CXCR4 is of great significance for COVID-19 patients with a history of AF.
Optimization of hydrogen production in Enterobacter aerogenes by Complex Ⅰ peripheral fragments destruction and maeA overexpression
Authors: Jiang Ke , Bai Ruoxuan , Gao Ting , Lu Ping , Zhang Jingya , Zhang Shuting , Xu Fangxu , Wang Shenghou , Zhao Hongxin 
Published: March 2023

Abstract
As a concentrated energy source with high added value, hydrogen has great development prospects, with special emphasis on sustainable microbial production as a replacement for traditional fossil fuels. In this study, λ-Red recombination was used to alter the activity of Complex I by single and combined knockout of nuoE, nuoF and nuoG. In addition, the conversion of malic to pyruvic acid was promoted by overexpressing the maeA gene, which could increase the content of NADH and formic acid in the bacterial cells. Compared to the original strain, hydrogen production was 65% higher in the optimized strain IAM1183-EFG/M, in which the flux of the formic acid pathway was increased by 257%, the flux of the NADH pathway was increased by 13%, and the content of metabolites also changed significantly. In further bioreactor, scale-up IAM1183-EFG/M also showed strong industrial application potential, with a total hydrogen production of 4.76 L after 44h of fermentation, which significantly increased by 18% compared with the starting strain. This study provides a new direction for future exploration of microbial hydrogen production by combinatorial modification of multiple genes.
Refining intra-patch connectivity measures in landscape fragmentation and connectivity indices
Authors: Justeau-Allaire Dimitri , Ibanez Thomas , Vieilledent Ghislain , Lorca Xavier , Birnbaum Philippe 
Published: March 2023

Abstract
Context. Measuring intra-patch connectivity, i.e. the connectivity within a habitat patch, is important to evaluate landscape fragmentation and connectivity. However, intra-patch connectivity is mainly measured with patch size, which can conceal diverse intra-patch connectivity patterns for similar patch size distributions.
Objectives. We suggest a method to refine the intra-patch connectivity component of fragmentation and connectivity indices. This method allows for distinguishing different intra-patch connectivity patterns for similar patch size distributions.
Methods. We used normalized patch complexity indices to weight patch size in common fragmentation and connectivity indices. Patch complexity indices included two existing geometrical indices (SHAPE and FRAC), and a new index derived from spatial network analysis, the mean detour index (MDI). We analyzed the behaviours of adjusted fragmentation and connectivity indices theoretically and empirically on both artificial and real landscapes.
Results. While maintaining the mathematical properties of fragmentation and connectivity indices, our method could distinguish landscapes with identical patch size distributions but different spatial configurations. The mean detour index had a different response than geometrical indices. This result indicates that, at the patch level, topological complexity can exhibit different patterns from geometrical complexity.
Conclusions. Measuring intra-patch connectivity with patch size in fragmentation and connectivity indices cannot distinguish landscapes having similar patch sizes distribution but different spatial configurations. This paper introduces a method to distinguish such patterns relying on geometrical and topological indices and shows to which extent it can impact conservation planning.
Robotic-assisted and Laparoscopic Splenectomy in Children: A Single Center Comparative Study
Authors: Zhang Yuebin , Zhang Shuhao , Chen Qingjiang , Cai Duote , Luo Wenjuan , Jin Yi , Gao Zhigang 
Published: March 2023

Abstract

Background
To explore the safety, efficacy, advantages and disadvantages of robotic-assisted splenectomy (RS) in children by analyzing and comparing the clinical data of RS and traditional laparoscopic splenectomy (LS).
Methods
The clinical data of 35 children who underwent laparoscopic or robotic assisted splenectomy or partial splenectomy from February 2010 to October 2022 were included. A retrospective analysis based on general information, clinical data and prognosis were performed.
Results
Among 35 cases, 14 cases and 21 cases underwent RS and LS, respectively. The average operation time was 167 (120 to 224) minutes in the RS group and 176 (166 to 188) minutes in the LS group, the average intraoperative blood loss was 20 (8.7 to 27.5) ml in the RS group and 51 (23.5 to 75.5) mL in the LS group, the average length of hospital stay was 8 (7 to 9.25) days in the RS group and 10 (9 to 12) days in the LS group; the average hospitalization cost was 69 (67 to 71) thousand RMB in the RS group and 32 (31 to 34) thousand RMB in the LS group. There were no cases of conversion to laparotomy in the RS group, but two cases in the LS group. In terms of postoperative complications, there were one and three cases in the RS and LS group, respectively.
Conclusion
The Robotic Surgical System was safe and feasible in pediatric splenectomy or partial splenectomy which had the advantages of shortening the operation and hospitalization time, reducing intraoperative blood loss, the rate of conversion to laparotomy and the incidence of postoperative complications, but RS had a significantly higher hospitalization cost than LS.
Impacts of 10 years of elevated CO2 and warming on soil fungal diversity and network complexity in a Chinese paddy field
Authors: Gao Ke , Li Weijie , Zhang Zhihui , Jiang Li , Liu Yuan 
Published: March 2023

Abstract
Fungal communities play essential roles in ecosystems and are involved in soil formation, waste decomposition, nutrient cycling, and plant nutrient supply. Although studies have focused on soil bacterial community responses to climate change in agricultural ecosystems, only few have investigated the dynamic changes in the diversity and complexity of fungal communities in paddy fields. Herein, using internal transcribed spacer (ITS) gene amplicon sequencing and co-occurrence network methods, the responses of soil fungal community to factorial combinations of elevated CO2 (550 ppm) and canopy warming (+2°C) were explored in an open-air field experiment in Changshu, China, for 10 years. Elevated CO2 significantly increased the operational taxonomic unit (OTU) richness and Shannon diversity of fungal communities in both rice rhizosphere and bulk soils, whereas the relative abundances of Ascomycota and Basidiomycota were significantly decreased and increased, respectively, by elevated CO2. Co-occurrence network analysis showed that elevated CO2, warming, and their combination increased the network complexity and negative correlation of the fungal community in rhizosphere and bulk soils, suggesting that these factors enhanced the competition of microbial species. Warming resulted in a more complex network structure by altering topological roles and increasing the numbers of key fungal nodes. Principal coordinate analysis indicated that rice growth stages rather than elevated CO2 and warming altered soil fungal communities. Specifically, the changes in diversity and network complexity were greater at the heading and ripening stages than at the tillering stage. Furthermore, elevated CO2 and warming significantly increased the relative abundances of pathotrophic fungi and reduced those of symbiotrophic fungi in both rhizosphere and bulk soils. Overall, the results indicate that long-term CO2 exposure and warming enhance the complexity and stability of soil fungal community, potentially threatening crop health and soil functions through adverse effects on fungal community functions.
Transient Plasma Viral Rebound After Sars-cov-2 Vaccination in an Exceptional Hiv-1 Elite Controller Woman
Authors: Girolamo Laura Di , Ferrara Micol , Trevisan Giulia , Longo Bianca Maria , Allice Tiziano , Burdino Elisa , Alladio Francesca , Fantino Silvia , Perri Giovanni Di , Calcagno Andrea , Bonora Stefano 
Published: March 2023

Abstract
Background
Elite controllers are able to control viral replication without antiretroviral therapy. Exceptional elite controllers do not show disease progression for more than 25 years. Different mechanisms have been proposed and several elements of both innate and adaptive immunity are implicated. Vaccines are immune stimulating agents that can promote HIV-RNA transcription; transient plasma HIV-RNA detectability has been described within 7-14 days after different vaccinations. The most reliable mechanism involved in virosuppressed people living with HIV is a generalized inflammatory response that activates bystander cells harboring latent HIV. So far no data about viral load increase in elite controllers after SARS-CoV-2 vaccination are reported in literature.
Case Presentation
We report the case of a 65-year-old woman of European ancestry, diagnosed with HIV-1/HCV co-infection more than 25 years ago. Since then, HIV-RNA remained undetectable and she
never received ARV therapy. In 2021 she was vaccinated with mRNA-BNT162b2 vaccine (Pfizer-BioNTech®). She was administered with three doses in June, July and October 2021, respectively.
The last available viral load was undetectable in March 2021. We observed an increase of VL at 32 cp/ml and 124 cp/mL, two and seven months after the first vaccine dose, respectively. During monthly follow-up, HIV-RNA gradually and spontaneously dropped becoming undetectable without ARV intervention. COVID-19 serology was positive with IgG 535 BAU/mL, showing response to vaccination.
We measured total HIV-DNA at different time-points and we found it detectable both at the time of the higher plasma HIV-RNA (30 cp/10^6 PBMCs) and when it was undetectable (13 cp/10^6 PBMCs), in reduction.
Conclusions
This case is the first report, to our knowledge, describing a rebound of plasma HIV-RNA in an elite controller after three doses of mRNA-BNT162b2 vaccine for SARS-CoV-2.
Concomitantly with a spontaneous reduction of plasma HIV-RNA ten months after the third dose of mRNA-BNT162b2 vaccine (Pfizer-BioNTech®) without antiretroviral therapy intervention, we observed a reduction of total HIV-DNA in peripheral mononuclear cells. The potential role of vaccinations in altering HIV reservoir, even in elite controllers population when plasma HIV-RNA is undetectable, could be a valuable aspect to take into account for the future HIV eradication interventions.
Music Recommendation Based on Current Mood Using Ai &amp; Ml
Authors: Roy Dharmendra , CH Anjali. , Kavya G. , Tharun B. , Gopal K. Venu 
Published: March 2023

Abstract
Music always has a special connection to our emotions. It is a method of connecting people all over the world through pure emotions. When all of this is considered, it is exceedingly difficult to generalize music and state that everyone would enjoy the same type. Music recommendation based on mood is greatly needed because they will assist humans in relieving stress and listening to soothing music based on their current emotions. Its primary goal is to accurately predict the user's mood, and songs are played by the application based on the user's selection as well as his current mood. This bot recognizes human emotions from facial images using Human-Computer Interaction (HCI). The extraction of facial elements from the user’s face is another critical factor. We used the CNN Algorithm to accurately detect the user's face in the live webcam feed and to detect emotion based on facial attributes such as the arrangement of the user's mouth and eyes. There is also a questionnaire where we can directly select a good option.
Genetic Profile of Schistosomes from Malian hotspot Areas with Urogenital Schistosomiasis
Authors: Agniwo Koba Privat , Bakary Sidibé , Diakité Assitan , Dembélé Laurent , Niaré Safiatou Doumbo , Guindo Hassim , Akplogan Ahristode Barthel , Ibikounlé Moudachirou , Boissier Jérôme , Spangenberg Thomas , Dabo Abdoulaye 
Published: March 2023

Abstract

Background - Although schistosomiasis is a public health issue in Mali, little is known about the genetic profile. The purpose of this study was to analyze the genetic profile of the schistosomes of Schistosoma haematobium group in school aged children in various sites of Mali.
Methods - Urine samples were collected from 07 to 21 November 2021 and subjected to a filtration method for the presence S. haematobium eggs. The study took place in two schistosomiasis endemic villages, qualified as hotspots according to the World Health Organization (WHO) definition Fangouné Bamanan and Diakalel in Kayes’ region. Molecular biology (Cox1 and ITS2/18S) was used as eggs taxonomic parameters.
Results - A total of 789 miracidium were collected individually from 63 school aged children and stored on Whatman Find The Agent (FTA) cards for molecular analysis. After genotyping for species and hybrid identification using rapid diagnostic multiplex mitochondrial Cox1 Polymerase chain reaction (PCR) and Amplification-refractory mutation system -ARMS_PCR analysis of the nuclear Internal Transcribed spacer - ITS2 and 18S regions, 338 (42.8%) and 45) (57.2%) of miracidium revealed S. bovis and S. haematobium profiles for Cox1, respectively; 749 (94.9%) and 40 (5.1%) revealed S. haematobium and S. haematobium/S. curassoni profiles for ITS/18S genes, respectively. There was a significant difference in the Cox1 and ITS2/18S profiles distribution between the two villages (p<0.0001). Overall, there was 360 (45.6%) cases of hybrids of which 322 (72.0%) were from Fangouné Bamanan. Three hybrids profiles [(Sb_ShxSc) with 2.3%); (Sb_ShxSh with 40,9%) ; (Sh_ShxSc with 2.8%) and one pure profile [Sh_ShxSh with 54.5%) were identified. The hybrid Sb_ShxSh appeared to be more frequent in Fangouné Bamanan (68%) whereas Sh_Sh/Sc was lightly represented in Diakalel (5.8%).
Conclusions - Our findings show for the first time the presence of introgressive hybridization between S. haematobium and S. bovis/S. curassoni in humans at a large scale. More studies are needed on population genetics of schistosomes at the human and animal interface to evaluate the parasite’s gene flow and its consequences on epidemiology of the disease as well as the transmission to humans.
Biomasonry products from macroalgae: a design driven approach to developing biomaterials for carbon storage
Authors: Scardifield Kate , McLean Nahum , Kuzhiumparambil Unnikrishnan , Ralph Peter J. , Neveux Nicolas , Isaac Geoff 
Published: March 2023

Abstract

Lowering the embodied carbon of building materials requires a transition away from fossil derived products towards bio-based alternatives, alongside the design and development of new clean tech biomaterials that can function as carbon sinks. This paper explores gaps and opportunities for the development of seaweed-based construction materials that can support atmospheric carbon removal through algal photosynthesis and provide carbon storage solutions within the built environment. Utilising a biorefinery framework our research aims to valorise residual seaweed biomass where it’s being grown for waste-water management and to identify value-added opportunities for this seaweed by-product. We present as a case study the design of seaweed ‘biobricks’ and the construction of a 1:1 scale prototype, demonstrating what biomasonry products from macroalgae can look like. Our paper highlights the value of interdisciplinary methodologies that combine materials science with design research, and the role of design prototypes in showcasing novel biomaterials and new sustainable forms of biodesign.
Probabilistic quantification in the analysis of groundwater drought in the Odra River Basin
Authors: Kubicz Justyna , Karczewski Maciej 
Published: March 2023

Abstract

As a result of increasing water deficits, low nutritional safety, and environmental degradation, the need to implement sustainable water usage and improve current water management practices became a priority. The changes in the hydroclimatic regimen towards more intense yet less frequent rainfall that have been observed in recent years increase the risk of drought. This leads to the need to mitigate this risk and to reduce the effects of repeated, consecutive periods of water shortage. The main aim of the study is to present the method of quantification of uncertain events, such as extreme groundwater levels. Following the probabilistic theory of extreme values, the authors verified the hypotheses: both the scale and nature of groundwater risk significantly change in the studied period and over the past 40 years, a rise in the groundwater drought risk in the Odra River Basin has been observed.
The application of probabilistic method allowed to draw interesting conclusions groundwater drought risk within the last 10 years has been very high, the lowest risk occurred between 1980 and 1989. Too few points make it impossible to draw conclusions about long-term trends. Meteorological study in those areas indicate a relation between groundwater levels and climate change in recent years, represented by various factors such as precipitation, temperature, humidity. The risk calculated in this way may be used to classify the areas in terms of the level of risk of the occurrence of groundwater drought.
Melt Processible Biodegradable Blends of Polyethylene Glycol Plasticized Cellulose Diacetate with Polylactic Acid and Polybutylene Adipate-co-Terephthalate
Authors: Tselana Bethuel M. , Muniyasamy Sudhakar , Ojijo Vincent O. , Mhike Washington 
Published: March 2023

Abstract

Enhancing the melt processability of cellulose is key to broadening its applications. This is done via derivatization of cellulose, and subsequent plasticization and/or blending with other biopolymers, such as polylactic acid (PLA) and polybutylene adipate terephthalate (PBAT). However, derivatization of cellulose tends to reduce its biodegradability. Moreover, traditional plasticizers are non-biodegradable. In this study, we report the influence of polyethylene glycol (PEG) plasticizer on the melt processibility and biodegradability of cellulose diacetate (CD) and its blends with PLA and PBAT. CD was first plasticized with PEG (PEG-200) at 35 wt.%, and then blended with PLA and PBAT using a twin-screw extruder. Blends of the PEG plasticized CD with PLA at 40 wt.% and with PBAT at 60 wt.% were studied in detail. Dynamic mechanical analysis (DMA) showed that PEG reduced the glass transition of the CD from ca. 220°C to less than 100°C, indicating effective plasticization. Scanning electron microscopy revealed that the CD/PEG-PBAT blend had a smoother morphology implying some miscibility. The CD/PEG-PBAT blend at 60 wt.% PBAT had an elongation-to-break of 734%, whereas the CD/PEG-PLA blend had a tensile strength of 20.6 MPa, comparable to that of the PEG plasticized CD. After a 108-day incubation period under simulated aerobic composting, the CD/PEG-PBAT blend at 60 wt.% PBAT exhibited a biodegradation of 41%, whereas that of the CD/PEG-PLA at 40 wt.% PLA was 107%. This study showed that melt processible, biodegradable CD blends can be synthesized through plasticization with PEG and blending with PBAT or PLA.
Phonetic Accommodation During Conversational Interactions: An Overview
Authors: Barón-Birchenall Leonardo 
Published: March 2023

During conversational interactions such as tutoring, instruction-giving tasks, verbal negotiations, or just talking with friends, interlocutors’ behaviors experience a series of changes due to the characteristics of their counterpart and to the interaction itself. These changes are pervasively present in every social interaction, and most of them occur in the sounds and rhythms of our speech, which is known as acoustic-prosodic accommodation, or simply phonetic accommodation. The consequences, linguistic and social constraints, and underlying cognitive mechanisms of phonetic accommodation have been studied for at least 50 years, due to the importance of the phenomenon to several disciplines such as linguistics, psychology, and sociology. Based on the analysis and synthesis of the existing empirical research literature, in this paper we present a structured and comprehensive review of the qualities, functions, onto- and phylogenetic development, and modalities of phonetic accommodation.