bluuebunny/crossref_metadata_2025_split huggingface dataset:
Comprehensive Multi-Omics Analysis Reveals NPC2 and ITGAV Genes as Potential Prognostic Biomarkers in Gastrointestinal Cancers
Authors: Piroozkhah Moein , Zabihi Mohammadreza , Jalali Pooya , Salehi Zahra 
Published: May 2023

Abstract
Background
Gastrointestinal cancers (GICs) continue to dominate in terms of both incidence and mortality worldwide. Due to the absence of efficient and accurate prognostic biomarkers, the prognosis and treatment outcomes of many GICs are poor. Identifying biomarkers to predict individual clinical outcomes efficiently is a fundamental challenge in clinical oncology. Although several biomarkers have been continually discovered, their predictive accuracy is relatively modest, and their therapeutic use is restricted. In light of this, the discovery of reliable biomarkers for predicting prognosis and outcome in GIC is urgently required.
Methods
We evaluated the HPA dataset and identified NPC2 and ITGAV as probable poor predictive genes for these cancers. In addition, we used the GEPIA2, cBioPortal, UALCAN, LinkedOmics, STRING, Enrichr, TISDB, TIMER2.0, hTFTarget, miRTarBase, circBank, and DGIdb databases to conduct a comprehensive and systematic analysis of the NPC2 and ITGAV genes.
Result
Our results found high expression levels of NPC2 and ITGAV in most GICs. The aforementioned gene expressions were linked to several clinicopathological characteristics of GICs as well as poorer prognosis in LIHC and STAD. The most common alteration type of NPC2 was amplification, and for ITGAV was deep deletion. Significant promotor hypermethylation was also seen in NPC2 and ITGAV in PAAD and COAD, respectively. For the immunologic significance, NPC2 and ITGAV were positively correlated with the abundance of tumor-infiltrating lymphocytes and macrophages. Furthermore, various immuno-modulators showed strong correlations with the expression of these genes. There were currently ten small molecule drugs targeting ITGAV.
Conclusion
Consequently, our bioinformatics analysis showed that NPC2 and ITGAV might be used as potential biomarkers to determine the prognosis of various GICs and are also related to immune infiltration.
A curious case of eroded citations and the current status of top-50 cited papers by Indian Periodontists- A bibliometric analysis
Authors: Baghele Om Nemichand , Nagime Shital Chandraprakash , Ugale Gauri Mahesh , Vandana Kharidi Ladman , Bhandari Vishnudas Dwarkadas 
Published: May 2023

Abstract
Context: The bibliometric studies on top-most articles in any subject are available and fascinating, but there is no research available analysing the fate of top-cited articles. The citation to a paper is a dynamic process which is dependent on multitude of factors and may indicate the direction of research.
Aims: To analyse using bibliometric approach the current status of top-50 cited papers by Indian Periodontists based on PubMed database published in 2014.
Settings and Design: Departments of Periodontology at Dental Council of India recognized Dental Institutes. A comparative follow-up Bibliometric citation analysis of publications by Indian Periodontists.
Methods and Material: All the 50-top cited articles were individually searched on PubMed and Google Scholar databases for their availability and citations. The citations received by these articles till 25/11/2022 by 11.00 am Indian Standard Time were subjected to detailed analyses.
Statistical analysis used: Descriptive statistics using mean, median, range, percentages and proportions.
Results: Total citations to the 50-articles were increased from 597 (2012) to 932 (2022) with an overall increase of 6.7 citations/ article. 17 (34%) articles showed decrease in citations as compared to 2012 numbers; a decline of 99 citations with an average of 5.8 citations/ article. One article showed no change, and the rest 32 (64%) noted an increase by 434, averaging 13.6 citations/ article. The topmost 2012 article was relegated to 10th spot in 2022 while it remained at 6th spot in Google Scholar citations. In 2022, the topmost received 57 PubMed and 466 Google Scholar citations.
Conclusions: A top-cited or classic article may not remain at that position through its life-cycle, but goes on receiving citations. There are instances of ‘erosion in citations’ as well which needs to be recognized and awareness amongst researchers is necessary. There is a need to identify current top-50 classic articles and compare them with these to understand focus on current research topics.
Filamentous virus-like particles are present in coral dinoflagellates across genera and ocean basins
Authors: Howe-Kerr Lauren I , Knochel Anna M , Meyer Matthew D , Sims Jordan A , Karrick Carly E , Grupstra Carsten GB , Veglia Alex J , Thurber Andrew R , Thurber Rebecca L Vega , Correa Adrienne MS 
Published: May 2023

Abstract
Filamentous viruses are hypothesized to play a role in stony coral tissue loss disease (SCTLD) through infection of the endosymbiotic dinoflagellates (Family Symbiodiniaceae) of corals. To evaluate this hypothesis, it is critical to understand the global distribution of filamentous virus infections across the genetic diversity of Symbiodiniaceae hosts. We present transmission electron microscopy images demonstrating filamentous virus-like particles (VLPs) are present in 61 and 70% of Symbiodiniaceae cells (genus Cladocopium) within Pacific corals (Acropora hyacinthus, Porites c.f. lobata, respectively); these VLPs are more prevalent in Symbiodiniaceae of in situ colonies experiencing heat stress. Symbiodiniaceae expelled from A. hyacinthus also contain filamentous VLPs, and these cells are more degraded than their in hospite counterparts. Similar to the VLPs reported from SCTLD-affected Caribbean reefs, VLPs range from ~ 150–1500 nm in length and 15–37 nm in diameter and appear to constitute various stages in a replication cycle. Finally, we demonstrate that SCTLD-affected corals containing filamentous VLPs are dominated by diverse Symbiodiniaceae lineages from the genera Breviolum, Cladocopium, and Durusdinium. Although this study cannot definitively confirm or refute the role of filamentous VLPs in SCTLD, it demonstrates that filamentous VLPs are not solely observed in SCTLD-affected corals or reef regions, nor are they solely associated with corals dominated by members of a particular Symbiodiniaceae genus. We hypothesize that filamentous viruses are a widespread, common group that infects Symbiodiniaceae. Genomic characterization of these viruses and empirical tests of the impacts of filamentous virus infection on Symbiodiniaceae and coral colonies should be prioritized.
Landmark Model-based Individual Dynamic Prediction of Conversion from Mild Cognitive Impairment to Alzheimer’s Disease using Cognitive
Screening
Authors: Cui Jing , Chen Durong , Zhang Jiajia , Qin Yao , Bai Wenlin , Ma Yifei , Zhang Rong , Yu Hongmei 
Published: May 2023

Background:
Identifying individuals with mild cognitive impairment (MCI) who are at increased
risk of Alzheimer’s Disease (AD) in cognitive screening is important for early diagnosis and
prevention of AD.

Objective:
This study aimed at proposing a screening strategy based on landmark models to provide
dynamic predictive probabilities of MCI-to-AD conversion according to longitudinal neurocognitive
tests.

Methods:
Participants were 312 individuals who had MCI at baseline. The longitudinal neurocognitive
tests were the Mini-Mental State Examination, Alzheimer Disease Assessment Scale-Cognitive 13
items, Rey Auditory Verbal Learning Test immediate, learning, and forgetting, and Functional Assessment
Questionnaire. We constructed three types of landmark models and selected the optimal
landmark model to dynamically predict 2-year probabilities of conversion. The dataset was randomly
divided into training set and validation set at a ratio of 7:3.

Results:
The FAQ, RAVLT-immediate, and RAVLT-forgetting were significant longitudinal neurocognitive
tests for MCI-to-AD conversion in all three landmark models. We considered Model 3 as
the final landmark model (C-index = 0.894, Brier score = 0.040) and selected Model 3c (FAQ and
RAVLT-forgetting as neurocognitive tests) as the optimal landmark model (C-index = 0.898, Brier
score = 0.027).

Conclusion:
Our study shows that the optimal landmark model with a combination FAQ and RAVLTforgetting
is feasible to identify the risk of MCI-to-AD conversion, which can be implemented in cognitive
screening.
Dihydrotanshinone I Inhibits Pancreatic Cancer Progression via Hedgehog/
Gli Signal Pathway
Authors: Huang Wenqian , Dai Yile , Xu Liming , Mao Yefan , Huang Zhengwei , Ji Xiaoke 
Published: May 2023

Introduction:
Pancreatic cancer is highly fatal and its incidence is rising worldwide. Its
poor prognosis is attributed to a lack of effective diagnostic and therapeutic strategies. Dihydrotanshinone
I (DHT), a phenanthrene quinone liposoluble compound from Salvia miltiorrhiza Bunge
(Danshen), exerts anti-tumor effects by inhibiting cell proliferation, enhancing apoptosis, and inducing
cell differentiation. However, its effects on pancreatic cancer are unclear.

Methods:
The role of DHT in the growth of tumor cells was explored using real-time cell analysis
(RTCA), colony formation assay, and CCK-8. The effects of DHT on tumor cells invasion as well as
migration were assessed by Transwell and migration assays. Expressions of pro-apoptosis and metastasis
factors in tumor cells were examined using western blot. Tumor apoptosis rates were studied using
flow cytometry. The anticancer effect of DHT in vivo was assessed by tumor transplantation into
nude mice.

Results:
Our analyses show that DHT has a suppressive role in epithelial-mesenchymal transition
(EMT), invasiveness, proliferation, as well as migratory ability of Patu8988 and PANC-1 cells via
Hedgehog/Gli signaling. Moreover, it drives apoptosis via caspases/BCL2/BAX signaling. Experiments
in nude mice transplanted with tumors have shown DHT to have anticancer effects in vivo.

Conclusion:
Our data show that DHT effectively suppresses pancreatic cancer cell proliferation as
well as metastasis, and induces apoptosis via Hedgehog/Gli signaling. These effects have been reported
to be dose- and time-dependent. Therefore, DHT can be exploited as a potential treatment for pancreatic
cancer.
Green Synthesis of Molecules for the Treatment of Neglected Diseases
Authors: Anghinoni João M. , Dilelio Marina C. , Shiguemoto Cristiane Y.K. , Schumacher Ricardo F. , Baroni Adriano C.M. , Lenardão Eder João 
Published: May 2023

Abstract:
Neglected tropical diseases (NTDs) affect mainly poor and marginalized populations of tropical and subtropical areas in 150 countries. Many of the chemical processes involved in the syn-thesis of active pharmaceutical ingredients (APIs) are highly polluting and inefficient, both in terms of materials and energy-consuming. In this review, we present the green protocols developed in the last 10 years to access new small molecules with potential applications in the treatment of leishma-nia, tuberculosis, malaria, and Chagas disease. The use of alternative and efficient energy sources, like microwaves and ultrasound, as well as reactions using green solvents and solvent-free proto-cols, are discussed in this review.
Dichlorophosphoryl Isocyanate: Synthesis, Chemical Reactions, and
Biological Activity of its Derivatives
Authors: Medini Faten , Aroua Lotfi Mohamed , Mekni Nejib Ben Hussein 
Published: May 2023

Abstract:
Dichlorophosphoryl isocyanate (DCPI) is the most basic and easy phosphoric isocyanate
substrate that introduces organic moieties. Synthesized since 1954, the DCPI has a high reactivity toward
primary and secondary alkyl, alcohols, phenols, thiols, and amines via the nucleophilic addition
reaction on the carbon atom of the isocyanate group. In addition to their synthesis, the resulting products
undergo nucleophilic substitutions of the chlorine atoms. Their reactions with nucleophilic and
bi-nucleophile reagents yield acyclic and P-heterocyclic compounds, respectively. The resulting compounds
have different potential antibacterial, antifungal, and antitumor activities.
Diagnosing Non–Producers Non–Secretory Myeloma
Authors: Nurarifah Nina , Wardhani Shinta 
Published: May 2023

Multiple myelomas (MM) is the proliferation of malignant plasma cells in the bone marrow, characterized by the production of monoclonal immunoglobulins that are secreted in the blood and urine. However, in 1 - 5% of cases, the protein is undetectable and defined as non-secretory type of myeloma.
A 36-year-old woman presented with complaints of weakness in both legs so she could not walk. She also felt pain in the head, back, and thighs since one year before. Physical examination showed anemic conjunctiva, gibus as high as T4, hypoesthesia as high as T6 segment. She had anemia (Hb 10.2 g/dL), decreased kidney function (eGFR: 30 mL/min/1.73m2), and multiple lytic lesions in the calvaria, ribs, superior-inferior pubic ramus, and left humeral os in the 1/3 proximal to 1/3 distal. Protein electrophoresis and Bence Jonce protein were negative. There was no hypercalcemia, and bone marrow aspiration showed myelodysplasia syndrome. A thoracolumbar MRI showed an extradural solid mass and the histopathology examination result was plasmacytoma.
Non-secretory MM was defined into two groups, non-producers and multiple non-secretory myeloma patients who produce tumor proteins but cannot be excreted. This patients was categorized as non-producers non-secretory MM because she did not show a protein that can be measured in blood nor urine, but has significant plasma cells in tissue biopsy.
The absence of paraprotein in the blood does not rule out multiple myeloma. Suspicion of multiple myeloma needs to be increased in patients who have symptoms of CRAB (calcium, renal impairment, anemia, bone lytic).
A review of sustainability applications in the construction industry: perspectives and challenges
Authors: Naji Alaa Jalil Naji , Elsheikh Asser Mohamed 
Published: May 2023

Introduction. One of the main causes of annual environmental degradation directly or indirectly is construction. The purpose of this study is to highlight current environmental issues in the construction industry. Besides how the construction sector can contribute to reducing the negative impact on the environment and creating “healthy buildings” that can be sustainable for both residents and our environment.
Materials and methods. An overall literature survey was carried out to get relevant information and provide an overview of the current situation regarding the applications of sustainability in the construction industry. According to the selected theme, an inclusive investigation was conducted using several databases, such as Google Scholar, Scopus, eLIBRARY.RU,and ScienceDirect. Many crucial key words, such as environmental sustainability, social sustainability, economic sustainability, sustainable development, environmental issues, etc. Were used to extract data on the sustainability issues in the construction industry. The primary data was compiled in the form of tables and figures. In most cases,the emphasis was on recent data.
Results. The most common methods for modelling sustainable construction indicators are described below, as well asthe sustainable construction materials that have been used recently.
Conclusions. Building information modelling (BIM) appears is the single most commonly used technology today. With the development of computer technology, BIM is increasingly being used in combination with other techniques to achieve sustainable building goals. Careful selection of sustainable building materials may be the quickest way for builders to start integrating sustainable building design concepts.
The Blogosphere as a Specific Area of Digital Communication
Authors: Chechulin A. V. , Babenko V. A. , Stepanova E. A. , Ivanova V. M. 
Published: May 2023

This article examines the specifics of the blogosphere of the Russian-speaking segment of the Internet, as well as the changes that have occurred in this area after the ban of certain social platforms and their recognition as extremist on the territory of the Russian Federation. The study was carried out using a comparative method and is based on the analysis of the content of blogs in the social networks VKontakte, Dzen, Telegram, selected by a continuous sampling method for the period from February 2022 to October 2022. The blogosphere today is a dynamically and actively developing environment, and blogs are no longer just a channel for broadcasting relevant information, but also a social and communicative phenomenon existing at the intersection of journalism, marketing and political technologies.The recognition of some popular social networks as extremist on the territory of the Russian Federation has led to a reduction in the income of bloggers associated with a sharp decrease in the coverage of their accounts, as well as the departure of a number of major foreign advertisers from the Russian market. There was a transition of the main bloggers to alternative blogging platforms, as well as the search for suitable forms of content submission for them and attempts to increase the audience. These changes affected not only bloggers, but also the accounts of various state budget organizations operating within the framework of the electronic state presented on the Internet.Social networks today are turning from platforms for communication and file sharing into digital ecosystems that include many services. Bloggers develop their accounts, increasing the number of subscribers and building up a regular audience and become influencers — opinion leaders, which is actively used by businesses in influencer marketing. To optimize cooperation with media personalities who have their own blogs, representatives of companies turn to influencer agencies, which today provide a full range of digital services, from the idea to its final implementation. The growth of the older audience leads to the fact that the content is “maturing” — more and more serious topics are being raised, the requirements for the quality of content are increasing. The trends of personalization of content and fragmentation of the blog audience are also increasing.
Targeted Ablation of Epicardial Ganglionated Plexi During Cardiac Surgery with Pulsed Field Electroporation ( NEURAL AF )
Authors: Musikantow Daniel , Reddy Vivek , Skalsky Ivo , Shaburishvili Tamaz , Zyl Martin van , O'Brien Barry , Coffey Ken , Reilly John , Neuzil Petr , Asirvatham Samuel , Groot Joris de 
Published: May 2023

Background: Modulation of the cardiac autonomic nervous system
(ANS) is a promising adjuvant therapy in the treatment of atrial
fibrillation (AF). In pre-clinical models, pulsed field (PF) energy has
the advantage of selectively ablating the epicardial ganglionated plexi
(GP) that govern the ANS. Objective: This study aims to demonstrate the
feasibility and safety of epicardial ablation of the GPs with PF during
cardiac surgery with a primary efficacy outcome of prolongation of the
atrial effective refractory period (AERP). Methods: In a
single-arm, prospective analysis, patients with or without a history of
AF underwent epicardial GP ablation with PF during coronary artery
bypass grafting (CABG). AERP was determined immediately pre- and post-
GP ablation to assess cardiac ANS function. Holter monitors were
performed to determine rhythm status and Heart Rate Variability (HRV) at
baseline and at 1 month post-procedure. Conclusions: This
study demonstrates the safety and feasibility of epicardial ablation of
the GP using PF to modulate the ANS during cardiac surgery. Large,
randomized analyses are necessary to determine whether epicardial PF
ablation can offer a meaningful impact on the cardiac ANS and reduce AF.
Results: Of 24 patients, 23 (96%) received the full ablation
protocol. No device-related adverse effects were noted. GP ablation
resulted in a 20.7% ± 19.9% extension in AERP (P < 0.001).
Post-operative AF was observed in 7 (29%) patients. Holter monitoring
demonstrated an increase in mean heart rate (74.0±8.7 vs
80.6±12.3, P=0.01). There were no significant changes in HRV. There were
no study-related complications.
INTERNALISASI PENDEKATAN HUMANISTIK CALON GURU MI/SD DI ABAD 21 PADA PERGURUAN TINGGI
Authors: Musyaffa A A , Jalal Muhaiminah , Aryastuti Hasanah Ika , Nirwana Iin 
Published: May 2023

Penelitian ini bertujuan untuk menganalisis proses internalisasi Pendekatan Humanistik Calon Guru MI/SD Di Abad 21 Pada Perguruan Tinggi. Pada penelitian ini peneliti menggunakan penelitian kualitatif deskriftif dengan melakukan wawancara,angket, observasi dan dokumentasi terkait life skill yang dimiliki mahasiswa calon guru. Tujuannya untuk mendeskripsikan life skills apa saja dan mendeskripsikan bagaimana keadaan life skills yang dimiliki mahsiswa calon guru. Hasil penelitian menunjukkan bahwa pertama, dosen menanamkan kepada diri mahasiswa yakni Self esteem approach, mengembangkan kepercayaan diri mahasiswa, Creatifity approach, mengembangkan potensi kreatif mahasiswa, Value clarification and moral, development approach, untuk membantu mahasiswa dalam mengembangkan proses-proses yang digunakan dalam menentukkan nilai siswa, Multiple talent approach. Kedua, dalam proses pembelajaran dengan melaksanakan aktivitas literasi pembelajaran dan guru memberikan penguatan karakter dalam proses pembelajaran dengan urutan kompetensi dari LOTS menuju kompetensi HOTS akan menghasilkan lulusan yang memiliki karakter dan kompetensi
SORPTION OF IONS OF SOME METAL ON CLINOPTILOLITE IN THE PRESENCE OF GALLIC ACID
Authors: Milyovich S.S. , Fizer M.M. , Stercho I.P. , Verteletskiy R.S. 
Published: May 2023

The Gibbs energy changes for the reaction between the copper, cadmium, and lead ions complexes with gallic acid and clinoptilolite containing single-charged (Na, K) and double-charged (Ca, Mg) exchange ions were calculated. The software PRIRODA with the PBE/3ζ theoretical method has been used for these calculations:
(GalAn)2X×2H2O + Clin-Me + 2H3O+ → X(H2O)n-Clin + 2GalAn– + (6-n)H2O + 2Me+(Me2+).
The thermodynamic possibility of spontaneous ion exchange between complexes of heavy metal ions (Cu2+, Cd2+, Pb2+) with gallic acid and exchange ions of clinoptilolite was shown. This indicates the possibility of removal of these ions from the soil, with their coordination by polyphenols. The possibility of a significant decrease in the content of Cu2+, Cd2+, and Pb2+ ions was investigated both in the absence of gallic acid and in its presence in solution (initial concentration of metal ions of 2 mmol/l, the ratio of Me2+: gallic acid is 1:0, 1:1, and 1:2). It has been found that the sodium form of clinoptilolite influences the growth of sorption properties of clinoptilolite, allowing to exclude 22% more copper ions, compared to the natural clinoptilolite of Sokyrnytsya deposit. Two mechanisms for reducing the concentration of heavy metals in soil have been proposed.
Keywords: clinoptilolite; sorption; heavy metal; quantum-chemical calculations; gallic acid.