bluuebunny/crossref_metadata_2025_split huggingface dataset:
Circ_0001387 regulates SKA2 to accelerate breast cancer progression through miR‐136‐5p
Authors: Xiong Youyi , Li Lin , Wang Nan , Wang Fang , Chen Zhuo , Han Luhong , Jiang Shan , Gu Yuanting 
Published: May 2023

AbstractBackgroundGrowing evidence has revealed the critical regulatory role for circular RNAs (circRNAs) in cancer. This study aimed to explore the function of circ_0001387 in breast cancer (BC).MethodsCirc_0001387, miR‐136‐5p, and spindle and kinetochore‐associated protein 2 (SKA2) levels were analyzed by quantitative real‐time polymerase chain reaction. Clone formation and 5‐ethynyl‐2′‐deoxyuridine assays were used to analyze cell proliferation. Cell apoptosis and cell migration and invasion abilities were analyzed using flow cytometry or transwell assay. Mechanism assay was used to confirm the association between miR‐136‐5p and circ_0001387 or SKA2. The effect of circ_0001387 on tumor growth in vivo was analyzed by the xenograft mice model.ResultsCirc_0001387 and SKA2 were expressed at high levels, whereas miR‐136‐5p was lowly expressed in BC tissues and cells. Meanwhile, the downregulation of circ_0001387 restrained BC cell progression in vitro and in vivo. Circ_0001387 competitively bound to miR‐136‐5p to regulate BC cell malignant behaviors. SKA2 was targeted by miR‐136‐5p, and SKA2 reinstated the suppressive effect of miR‐136‐5p upregulation in BC cells.ConclusionOur study indicated that circ_0001387 contributed to BC cell progression through miR‐136‐5p/SKA2 axis.
Influence of voxel size and filter type on detecting vertical root fracture using cone‐beam computed tomography
Authors: Kolsuz Mehmet Eray , Evli Cengiz , Aksoy Umut , Koohnavard Mehrdad , Aksoy Seçil , Orhan Kaan 
Published: May 2023

AbstractVertical root fractures (VRFs) can start at any level of the root and progress longitudinally to the coronal attachment. This study aimed to investigate the effects of different exposure parameters used when obtaining CBCT scans in detecting simulated VRFs. Hence, 80 intact human mandibular single‐rooted pre‐molar teeth without root fractures were included in the study. No statistically significant difference was found between the filters in terms of VRF detection in the group with the roots with only root canal filling (Groups 1 and 5); however, 100 voxels were found to be more successful in terms of VRF detection than other voxel sizes. Results of this study suggest that using lower voxel sizes leads to an accurate diagnosis of vertical root fracture, in addition, our results revealed that using AR filters did not improve the diagnostic accuracy in detecting VRFs.
Radiographic evaluation of the tenting screw technique in horizontal alveolar bone augmentation: A retrospective study
Authors: Yang Sijia , Wang Siyuan , Shen Xiaoting , Xu Yuzi , Chen Cong , He Fuming 
Published: May 2023

AbstractObjectivesTo radiographically analyze the effects of tenting screw technique (TS) and onlay bone grafts (OG) in horizontal bone augmentation.Materials and MethodsPatients receiving horizontal bone augmentation by TS or OG were selected. The clinical outcomes and cone beam computed tomography (CBCT) data were documented pre‐grafting, immediately post‐grafting, before and after implantation. The survival rates, clinical complications, alveolar bone width, and volumetric bone augmentation were evaluated and statistically analyzed.ResultsA total of 25 patients and 41 implants were involved in this study, with no grafting failures observed in either the TS group (n = 20) or the onlay group (n = 21). Volumetric bone resorption rate in the TS group (21.34%) was significantly lower than that of the OG group (29.38%). In addition, significant horizontal bone gain was achieved in both groups (TS: 6.15 ± 2.12 mm; OG: 4.86 ± 1.40 mm) during the recovery period, with higher gain in the TS group. No apparent statistical difference in terms of volumetric bone gain was observed between the TS (748.53 mm3, 607.47 mm3) and OG group (811.77 mm3, 508.49 mm3) immediately post‐grafting or after the recovery period.ConclusionBoth TS and OG achieved satisfactory bone augmentation effects, yet TS resulted in more bone augmentation and better stability than OG, with a reduced use of autogenous bone. Overall, the tenting screw technique can serve as an effective alternative to autogenous bone grafts.
Assessing the impact of providing digital product information on the health risks of alcoholic beverages to the consumer at point of sale: A pilot study
Authors: Rehm Jürgen , Ferreira‐Borges Carina , Kokole Daša , Neufeld Maria , Olsen Aleksandra , Rovira Pol , Segura Garcia Lidia , Tran Alexander , Colom Joan 
Published: May 2023

AbstractIntroductionThere is an ongoing policy debate in the European Union regarding the best method of providing information to consumers on the health risks of alcohol use. One of the proposed channels is via the provision of QR codes. This study tested the usage rate of QR codes placed on point‐of‐sale signs in a supermarket in Barcelona, Catalonia over a 1‐week period.MethodsNine banners with beverage‐specific health warnings in large text were prominently displayed in the alcohol section of a supermarket. Each banner provided a QR code of relatively large image size that linked to a government website providing further information on alcohol‐related harms. A comparison was made between the number of visits to the website and the number of customers in the supermarket (number of unique sales receipts) in a single week.ResultsOnly 6 out of 7079 customers scanned the QR code during the week, corresponding to a usage rate of 0.085%, less than 1 per 1000. The usage rate was 2.6 per 1000 among those who purchased alcohol.Discussion and ConclusionsDespite the availability of prominently displayed QR codes, the overwhelming majority of customers did not make use of the QR codes to obtain further information on alcohol‐related harms. This corroborates the results from other studies investigating customers' use of QR codes to obtain additional product information. Based on the current evidence, providing online access to information through QR codes will likely not reach a significant portion of consumers.
Fine‐scale spatial and temporal distribution patterns of large marine predators in a biodiversity hotspot
Authors: Stephenson Fabrice , Hamilton Olivia N. P. , Torres Leigh G. , Kozmian‐Ledward Lily , Pinkerton Matt H. , Constantine Rochelle 
Published: May 2023

AbstractAimLarge marine predators, such as cetaceans and sharks, play a crucial role in maintaining biodiversity patterns and ecosystem function, yet few estimates of their spatial distribution exist. We aimed to determine the species richness of large marine predators and investigate their fine‐scale spatiotemporal distribution patterns to inform conservation management.LocationThe Hauraki Gulf/Tīkapa Moana/Te Moananui‐ā‐Toi, Aotearoa/New Zealand.MethodsWe conducted a replicate systematic aerial survey over 12 months. Flexible machine learning models were used to explore relationships between large marine predator occurrence (Bryde's whales, common and bottlenose dolphins, bronze whaler, pelagic and immature hammerhead sharks) and environmental and biotic variables, and predict their monthly distribution and associated spatially explicit uncertainty.ResultsWe revealed that temporally dynamic variables, such as prey distribution and sea surface temperature, were important for predicting the occurrence of the study species and species groups. While there was variation in temporal and spatial distribution, predicted richness peaked in summer and was the highest in coastal habitats during that time, providing insight into changes in distributions over time and between species.Main ConclusionsTemporal changes in distribution are not routinely accounted for in species distribution studies. Our approach highlights the value of multispecies surveys and the importance of considering temporally variable abiotic and biotic drivers for understanding biodiversity patterns when informing ecosystem‐scale conservation planning and dynamic ocean management.
Religious Organizations and Government: An Ecclesial Lutheran “Take”
Authors: Willer Roger A. 
Published: May 2023

AbstractSharp debates exist today about the appropriate relationship between religious organizations and government, with views ranging from Christian nationalism to strict secularists. This essay explores a mediating position expressed in the conceptual phrase that religious bodies should “work with civil authorities in areas of mutual endeavor, maintaining institutional separation and functional interaction.” The essay uses historical and ecclesial sources to describe the struggles and underpinnings that lead to this position and then unpacks its meaning and import. While originating from within the conceptual and ecclesial American Lutheran tradition, the essay points to this approach as a public proposal for sound relationship between religious communities and political authority in the United States.
Enhancing Arabic‐text feature extraction utilizing label‐semantic augmentation in few/zero‐shot learning
Authors: Basabain Seham , Cambria Erik , Alomar Khalid , Hussain Amir 
Published: May 2023

AbstractA growing amount of research use pre‐trained language models to address few/zero‐shot text classification problems. Most of these studies neglect the semantic information hidden implicitly beneath the natural language names of class labels and develop a meta learner from the input texts solely. In this work, we demonstrate how label information can be utilized to extract enhanced feature representation of the input text from a Transformer‐based pre‐trained language model such as AraBERT. In addition, how this approach can improve performance when the data resources are scarce like in the Arabic language and the input text is short with little semantic information as is the case using tweets. The work also applies zero‐shot text classification to predict new classes with no training examples across different domains including sarcasm detection and sentiment analysis using the information in the last layer of a trained classifier in a transfer learning setting. Experiments show that our approach has a better performance for the few‐shot sentiment classification compared to baseline models and models trained without augmenting label information. Moreover, the zero‐shot implementation achieved an accuracy up to 0.874 in Arabic sarcasm detection from a model trained on a sentiment analysis task.
Numerical simulation of the effect of surface microgeometry and residual stress on conformal contact fretting fatigue crack initiation behavior
Authors: Song Yifan , Yan Pei , Jiao Li , Gu Huiqing , Guo Zhibo , Zhao Bin , Wang Xibin 
Published: May 2023

AbstractConformal contact is a commonly presented contact form in assemblies. Non‐proportional loading is the main characteristics of conformal contact, which leads to prominent difficulty in revealing fretting crack behavior. In this paper, a finite element prediction model for Ti‐6Al‐4V pin‐hole contact fretting fatigue crack initiation was developed, which simultaneously considered the effect of fretting wear, surface roughness, surface skewness, surface kurtosis, and residual stress. The results show that phase differences of stress component, change in direction of principal stress, and high stress gradient are the main reasons for the initiation of fretting fatigue under conformal contact condition. The model based on the Fatemi–Socie (FS) parameter successfully predicted the location, orientation, and fatigue life of crack initiation, which agrees well with the experimental results. Additionally, machining‐induced residual stress can effectively inhibit mode I crack initiation at valleys. Moreover, ignoring the surface microgeometry characteristics reduces the prediction accuracy of the crack behavior.
Grassroots temporary urbanism as a challenge to the city of austerity? Lessons from a self‐organised park in Thessaloniki, Greece
Authors: Kapsali Matina 
Published: May 2023

AbstractThis paper traces the legacies of austerity through a focus on grassroots temporary urban interventions in public spaces. Drawing on a self‐organised neighbourhood park in Thessaloniki, Greece, it explores and analyses the politicising dynamics of these urban experiments that emerged as a direct response to state retrenchment but became a long‐term legacy of austerity with material and social consequences. The aim of the paper is to contribute to contemporary scholarly debates on temporary grassroots urbanism amidst austerity by uncovering the ambiguous nature of such urban experiments without reproducing dichotomising approaches that understand them as either a vehicle of neoliberalisation or an instrument of revolt. Through the case of Svolou I develop a twofold argument. First, regarding their social dynamics and legacies, grassroots urban initiatives that lack a politicising element of disagreement with (planning and governance) authorities result in creating parallel micro‐spheres of socialities and solidarities with a questionable long‐term impact against austerity urbanism. Although such socialities are important at the local level, they often risk reproducing neoliberal rationalities of individual responsibility and exclusions. Second, regarding their material legacies, I argue that grassroots urban initiatives produce temporary self‐organised spaces and experiment with innovative grassroots space‐making processes but they often remain entrapped at the local level without being able to develop broader contestations of the uneven urban development model emerging in and through austerity.
Quick and affordable DNA cloning by reconstitution of Seamless Ligation Cloning Extract using defined factors
Authors: Liu Alexander Y. , Koga Hiroto , Goya Chihiro , Kitabatake Makoto 
Published: May 2023

AbstractThe cloning of DNA fragments to plasmid vectors is at the heart of molecular biology. Recent developments have led to various methods utilizing homologous recombination of homology arms. Among them, Seamless Ligation Cloning Extract (SLiCE) is an affordable alternative solution that uses simple Escherichia coli lysates. However, the underlying molecular mechanisms remain unclear and the reconstitution of the extract by defined factors has not yet been reported. We herein show that the key factor in SLiCE is Exonuclease III (ExoIII), a double‐strand (ds) DNA‐dependent 3′‐5′ exonuclease, encoded by XthA. SLiCE prepared from the xthAΔ strain is devoid of recombination activity, whereas purified ExoIII alone is sufficient to assemble two blunt‐ended dsDNA fragments with homology arms. In contrast to SLiCE, ExoIII is unable to digest (or assemble) fragments with 3′ protruding ends; however, the addition of single‐strand DNA‐targeting Exonuclease T overcomes this issue. Through the combination of commercially available enzymes under optimized conditions, we achieved the efficient, reproducible, and affordable cocktail, “XE cocktail,” for seamless DNA cloning. By reducing the cost and time required for DNA cloning, researchers will devote more resources to advanced studies and the careful validation of their own findings.
Long‐term efficacy and risk factors of balloon‐assisted maturation for radial‐cephalic arteriovenous fistula with small‐caliber veins
Authors: Kao Tsung‐Chi , Hsieh Hung‐Chang , Yu Sheng‐Yueh , Su Ta‐Wei , Ko Po‐Jen 
Published: May 2023

AbstractIntroductionBalloon angioplasty maturation (BAM) is a salvage method for autologous arteriovenous fistula (AVF) maturation failure. AVF creation using small‐diameter veins is considered to have poor outcomes. Therefore, this study aimed to explore the long‐term patency of small‐diameter veins (≤3 mm) using BAM.MethodsBAM was performed if the fistula failed to mature and function adequately to provide prescribed dialysis.FindingsOut of 61 AVFs, 22 AVFs successfully matured without further intervention (AVF group) and 39 AVFs failed to mature. Except for 1 patient who required peritoneal dialysis, the remaining 38 patients received salvage BAM, and 36 of those successful matured (BAM group). Kaplan–Meier analysis revealed no significant differences between the AVF and BAM groups in terms of primary functional patency (p = 0.503) and assisted functional patency (p = 0.499). Compared with the AVF group, the BAM group had similar assisted primary functional patency (1‐year: 94.7% vs. 93.1%; 3‐year: 88.0% vs. 93.1%; 5‐year: 79.2% vs. 88.3%). In addition, there were no significant difference between groups in the duration of primary functional patency and assisted primary functional patency (p > 0.05). Multivariate analyses showed that vein diameter and number of BAM procedures were independent predictors of primary functional patency in the AVF group and BAM group, respectively. Patient with 1 mm increase in vein size had 0.13‐fold probability of having decreased duration of patency (HR = 0.13, 95% CI: 0.02–0.99, p = 0.049), while patients who received two times of BAM procedures were 2.885 as likely to have decreased duration of primary functional patency (HR = 2.885, 95% CI: 1.09–7.63, p = 0.033) than patients who received one BAM procedure.DiscussionBAM is a relatively effective salvage management option with an acceptable long‐term patency rate, even for small cephalic veins.
States and hotspots in Helicobacter pylori research from 2002 to 2021: A bibliometric analysis
Authors: Wang Ruiyu , Huang Shu , Gan Peiling , Pan Xiao , Wang Ping , Zhong Xiaolin , Lü Muhan , Zhou Xian , Tang Xiaowei 
Published: May 2023

AbstractBackgroundRecently, numerous publications on Helicobacter pylori (H. pylori) have been published, but bibliometric analyses on this research field are scarce. To address this gap, we conducted a bibliometric analysis to provide a comprehensive overview and to explore the current research states and hotspots in this field.Materials and MethodsPublications on H. pylori from 2002 to 2021 were retrieved from the Web of Science Core Collection database (WoSCC). Trends in publications and citations were analyzed using Excel 2021. VOSviewer and Citespace were used to perform bibliometrics analysis.Results36,266 publications on H. pylori were retrieved from the WoSCC database. In general, we observed an increasing trend in the number of publications over the past 20 years. The United States was the most productive and influential country, with the largest proportion of both publications and total citations. Helicobacter, US Department of Veterans Affairs, and Graham, David were the most productive journals, institutions and authors, respectively. Further analysis the co‐occurrence and burst detection of keywords revealed that the most common keywords were “Helicobacter pylori,” “gastric cancer,” and “gastritis,” all keywords were divided into eight main clusters, and the most important current research hotspot was the relationship between H. pylori infection and the changes of gut microbiota.ConclusionsThe United States has been the most productive and influential country on H. pylori research, and H. pylori‐related research remains an active research field. The relationship between H. pylori infection and the changes of gut microbiota is a research hotspot attracting significant attention.
Impact of nephroureterectomy on postoperative renal function in upper tract urothelial carcinoma: A multicenter retrospective study
Authors: Okuyama Yoshiharu , Hatakeyama Shingo , Tabata Ryuji , Fujimori Daiji , Kawashima Yohei , Tanaka Toshikazu , Fujita Naoki , Okamoto Teppei , Mori Kazuyuki , Yamamoto Hayato , Yoneyama Takahiro , Hashimoto Yasuhiro , Matsuoka Toshimitsu , Sato Satoshi , Ohyama Chikara 
Published: May 2023

ObjectiveTo assess the impact of radical nephroureterectomy on postoperative renal function in patients with upper tract urothelial carcinoma (UTUC).MethodsWe retrospectively evaluated 645 patients with UTUC treated with radical nephroureterectomy between January 2000 and May 2022. The primary outcome was the rate of postoperative estimated glomerular filtration rate (eGFR) ≥60 mL/min/1.73 m2. Secondary outcomes included the rate of eGFR decline, identification of factors related to eGFR decline, and the impact of comorbidities (diabetes or cardiovascular disease) on postoperative eGFR at 1 year.ResultsThe median preoperative and postoperative eGFR levels were 55.6 and 43.3 mL/min/1.73 m2, respectively. The rate of patients with preoperative and postoperative eGFR ≥60 mL/min/1.73 m2 was 40.9% and 9.0%, respectively. The median decline in eGFR after surgery was 25.1%. The presence of preoperative unilateral hydronephrosis and eGFR <60 mL/min/1.73 m2 was significantly associated with a low decline of postoperative eGFR and poor survival. The impact of the presence of comorbidities on postoperative eGFR at 1 year was significant (p < 0.001).ConclusionImpaired renal function is prevalent in patients with UTUC. The rate of patients with postoperative eGFR ≥60 mL/min/1.73 m2 was 9.0%. The presence of preoperative renal impairment was significantly related to a low decline in postoperative eGFR and poor survival. The presence of comorbidities had a significant effect on eGFR decline 1 year after radical nephroureterectomy.
Lyn‐mediated glycolysis enhancement of microglia contributes to neuropathic pain through facilitating IRF5 nuclear translocation in spinal dorsal horn
Authors: Kong Erliang , Li Yongchang , Ma Peng , Zhang Yixuan , Ding Ruifeng , Hua Tong , Yang Mei , Yuan Hongbin 
Published: May 2023

AbstractThe pro‐inflammatory phenotype of microglia usually induces neuroinflammatory reactions in neuropathic pain. Glycometabolism shift to glycolysis can promote the pro‐inflammatory phenotype transition of microglia. The omics data analysis suggest a critical role for Lyn dysregulation in neuropathic pain. The present study aimed at exploring the mechanism of Lyn‐mediated glycolysis enhancement of microglia in neuropathic pain. Neuropathic pain model was established by chronic constriction injury (CCI), then pain thresholds and Lyn expression were measured. Lyn inhibitor Bafetinib and siRNA‐lyn knockdown were administrated intrathecally to evaluate the effects of Lyn on pain thresholds, glycolysis and interferon regulatory factor 5 (IRF5) nuclear translocation of microglia in vivo and in vitro. ChIP was carried out to observe the binding of transcription factors SP1, PU.1 to glycolytic gene promoters by IRF5 knockdown. Finally, the relationship between glycolysis and pro‐inflammatory phenotype transition of microglia was evaluated. CCI led to the upregulation of Lyn expression and glycolysis enhancement in microglia of spinal dorsal horn. Bafetinib or siRNA‐lyn knockdown intrathecally alleviated pain hyperalgesia, suppressed glycolysis enhancement and inhibited nuclear translocation of IRF5 in CCI mice. Also, IRF5 promoted the binding of transcription factors SP1, PU.1 to glycolytic gene promoters, and then the enhanced glycolysis facilitated the proliferation and pro‐inflammatory phenotype transition of microglia and contributed to neuropathic pain. Lyn‐mediated glycolysis enhancement of microglia contributes to neuropathic pain through facilitating IRF5 nuclear translocation in spinal dorsal horn.