bluuebunny/crossref_metadata_2025_split huggingface dataset:
. The moderated ct angiography of the heart scanning protocol for children with dilated cardiomyopathy
Authors: Rudenko N. N. , Skripnik A. Yu. , Istomina V. V. , Zhuravel A. V. , Alkhazishvili A. V. , Shtentsel R. E. , Vershinina T.  L. , Vasichkina E. S. , Borshevetskaya A. A. , Fokin V. A. , Lukin M. V. 
Published: March 2023

Purpose. To moderate computed tomography angiography (CTA) of the heart scanning protocol in children with dilated cardiomyopathy and low left ventricle ejection fraction (LVEF) for optimal visualization of the heart and its major vessels.Materials and methods. We examined 38 patients aged from 9 months to 17 years, undergoing treatment at the cardiology department in the of the Rehabilitation Clinic for Children of the Almazov National Medical Research Centre. Firstly, all patients were performed echocardiography (Echo). Echo data evaluated LVEF. The patients were selected based on the clinical diagnosis, the LVEF index, the patient’s height (or the length of the scan area). 2 groups of patients with DCM (with low LVEF) were formed - younger and older patients; for each group was formed a control group of patients with normal LVEF values. All patients underwent CTA on a 128-sliced computed tomograph Ingenuity Elite (Philips, Netherlands) after a bolus intravenous contrast medium injection.Results. There was revealed a relationship between the start time of the scan and the LVEF, the patient’s height. For patients with low LVEF were created study protocols, where scan start time offset was set as: for the group 1 in the early arterial phase +3c, in the late arterial phase +5c, in the venous phase +15c; for the group 2 in the early arterial phase +5c, in the late arterial phase +5c, in the venous phase +29c.Conclusion. The moderated scanning protocol presents good contrast enhancement by chambers of the heart and its major vessels. It is necessary for an assessment of the anatomy and pathological changes. The identified criteria (LVEF, patient height) should be considered for CTA of the heart in children with dilated cardiomyopathy.
Panel of primers for evaluation of antibiotic resistance genes with real time detection of results
Authors: Mokhov A. S. , Klimova A. D. , Azarov D. V. , Goncharov A. E. 
Published: March 2023

Introduction. The problem of the spread of antibiotic resistance among pathogens of nosocomial infections is becoming increasingly important. In order to improve microbiological monitoring, it is advisable to use methods that allow you to quickly determine the largest number of antibiotic resistance determinants. In this regard, it seems relevant to develop PCR test systems for detecting antibiotic resistance genes, in particular, for screening hospitalized patients and identifying cases of nosocomial infection.Purpose. Develop a panel of primers for detection of antibiotic resistance genes using PCR with real-time visualization of results. Materials and methods. For the design of specific primers and probes, reference sequences from the databases of the National Center for Biotechnology Information (NCBI) were used. Primer selection and specificity assessment were performed using the NCBI PrimerBlast and Primer3 programs. Primers for PCR amplification of a number of antibiotic resistance genes were tested on strains of Klebsiella pneumoniae. These strains were isolated from the clinical material of intensive care patients with COVID-19 and whole genome sequencing of the strains was carried out with a detailed assessment of the resistome, virulome. The results of whole genome sequencing and multiplex real-time PCR were compared.Results. We have developed a set of primers for the detection of antibiotic resistance genes, including carbapenemase, using real-time multiplex PCR.Conclusion. The developed panel of primers can be used to screen Klebsiella pneumoniae isolates for the presence of resistance genes; further expansion of the spectrum of detected genes and testing of the panel on clinical material is required.
Astrocytes express aberrant immunoglobulins
as putative gatekeeper of astrocytes to neuronal progenitor conversion
Authors: Capuz Alice , Osien Sylvain , karnoub Melodie , Aboulouard Soulaimane , Laurent Estelle , Coyaud Etienne , Romero Antonella Raffo , Duhamel Marie , Bonnefond Amelie , Derhourhi Mehdi , Trerotola Marco , YAZIDI IKRAM EL , Devos David , Zilkova Monika , Kobaissy Firas , Abeele Fabien Vanden , Fournier Isabelle , Cizkova Dasa , Rodet Franck , Salzet Michel 
Published: March 2023

Abstract
Using multi-omics analyses including RNAseq, RT-PCR, RACE-PCR, and shotgun proteomic with enrichment strategies, we demonstrated that newborn rat astrocytes produce neural immunoglobulin constant and variable heavy chains as well as light chains. However, their edification is different from the ones found in B cells and resembles aberrant immunoglobulins as observed in several cancers. Moreover, the complete enzymatic V(D)J recombination complex has also been identified in astrocytes. In addition, the constant heavy chain is also present in astrocytes adult rats, whereas in primary astrocytes from human fetal astrocytes we identified constant and variable kappa chains as well as the substitution lambda chains known to be involved in pre-B cells. To gather insights into the function of these neural IgGs, CRISPR-Cas9 IgG2B constant heavy chain (IgH6), overexpression, proximal labeling of rat astrocytes IgH6 and targets identification through 2D gels were performed. In CRISPR-Cas9 IgG2B KO, overexpression of factors involved in hematopoietic cells, neural stem cells and the regulation of neuritogenesis have been identified. Moreover, overexpression of IgG2B in astrocytes induces the CRTC1-CREB-BDNF signaling pathway known to be involved in gliogenesis whereas CRISPR-Cas9 IgG2B KO triggers the BMP/YAP1/TEAD3 pathway activated in astrocytes dedifferentiation into neural progenitors. Proximal labeling experiments revealed that IgG2B is N-glycosylated by the OST complex, addressed to vesicle membranes containing the ATPase complex; and behaves partially like CD98hc through its association with LAT1. Proximity-dependent biotin labeling experiments suggest that proximal IgG2B-LAT1 interaction occurs concomitantly with MACO-1 and C2CD2L, at the heart of a potentially novel cell signaling platform. Finally, we demonstrated that these chains are synthesized individually and associated to recognize specific targets. Indeed, intermediate filaments Eif4a2 and Pdia6 involved in astrocyte fate constitute targets for these neural IgGs. Taken together, we hypotheses that aberrant neural IgG chains may act as gatekeepers of astrocytes fate.
The influence of trailing edge shape on the wake circulation and time-averaged wake of bio-inspired pitching panels
Authors: King Justin , Green Melissa 
Published: March 2023

Abstract
The influence of trailing edge shape and Strouhal number on the performance and wakes of bio-inspired pitching panels is investigated.
Measurements of thrust and efficiency show that positive mean thrust and efficiency only develop for panels of reduced aspect ratio that have straight or pointed trailing edges, and high aspect ratio panels with forked trailing edges fail to produce positive mean thrust.
Wake behaviors reveal that forked panels can produce strong trailing edge vortices while minimizing the three-dimensional effects of tip vortices, yet still do not generate positive mean thrust.
Regardless of planform, the time-averaged wake contains one or more jets of surplus streamwise momentum when Strouhal number is large enough.
As Strouhal number and trailing edge convexity increase, streamwise momentum in the time-averaged wake grows, and different jet structures may arise.
Multiple jets of large inclination angles can be present during high performance, and single or dual jets of negligible or small inclination angles may be present during degraded performance.
Just as recent work has demonstrated that direct connections between time-varying vortex patterns and mean performance are difficult to establish, caution must be used when connecting time-averaged jet structures to performance characteristics.
Shocked To Death: A Case Report Of Cardiogenic Shock And Death Following Electrocardioversion For Atrial Fibrillation
Authors: Hashem Anas Mohamad , Ali Omar Al , Shehadah Ahmed , Mohammed Moghniuddin , Khalouf Amani , Laguio-Vila Maryrose , Rao Mohan , Mahmoud Amir 
Published: March 2023

Abstract
Background:
Atrial fibrillation (AF) is prevalent, especially in patients with heart failure. Their prevalence increases with age and both conditions are interrelated. Electrocardioversion (ECV) is considered a safe and effective procedure and is among one of the recommended therapies to terminate AF back to normal sinus rhythm. Our study highlights one of the rare complications following ECV.
Case summary:
A 71-year-old female with a history of atrial fibrillation underwent electrocardioversion and developed sudden onset of ventricular stunning resulting in refractory cardiogenic shock. She was treated with mechanical cardiac support including IABP and Impella. Both provided minimal support then rapid clinical deterioration happened leading to imminent death.
Conclusion:
Patients with atrial fibrillation and heart failure treated with electrocardioversion might develop refractory cardiogenic shock and death as a complication of this procedure.
Oncogenic KRAS mutation confers chemoresistance by upregulating SIRT1 in non-small cell lung cancer
Authors: Shin Dong Hoon , Jo Jeong Yeon , Choi Minyoung , Kim Kyung-Hee , Bae Young-Ki , Kim Sang Soo 
Published: March 2023

Abstract
Kirsten rat sarcoma viral oncogene homologue (KRAS) is a frequent oncogenic driver of solid tumors, including non-small cell lung cancer (NSCLC). Its treatment and outcomes have been dramatically revolutionized by direct targeted therapies because of the lack of deep binding pockets for specific small -molecule inhibitors. Here, we demonstrated that the class III histone deacetylase SIRT1 mRNA and protein levels were upregulated by the KRASMut-Raf-MEK-c-Myc axis in KRASMut lung cancer cells and lung tumors of a spontaneous KrasG12D expression mouse model. KRASMut-induced SIRT1 bound to KRASMut again and stably deacetylased KRASMut lysine 104, which increased KRASMut activity. SIRT1 knock down (K/D) or SIRT1H363Y increased KRASMut acetylation, which decreased KRASMut activity, which sensitized the anti-cancer effect of cisplatin and erlotinib. Furthermore, KrasG12D/+;Sirt1co/co mice treated with cisplatin and erlotinib robustly reduced tumor burden and increased survival rates compared with these in spontaneous LSL-KrasG12D/+;Sirt1+/+ mice and each drug treatment group. By extension, we found p300 as a KRASMut acetyltransferase, which reinforced KRASMut lysine 104 acetylation and robustly decreased KRASMut activity. KRASMut lysine 104 acetylation by p300 and deacetylation by SIRT1 was confirmed by LC-MS/MS. Consistent with this finding, SIRT1 inhibitor, EX527 repressed KRASMut acetylation and activity, which abolished synergistically cell proliferation, colony formation, and tumor burden of KRASMut combined treatment with cisplatin or erlotinib. Our data reveals a novel pathway critical for the regulation of KRASMut lung cancer activity and provides important evidence for the potential application of SIRT1 inhibitor and p300 activator for the combination regimen of KRASMut lung cancer patients.
Significance
KRASMut lung cancer activity dependents on acetylation or deacetylation status at lysine 104 by SIRT1 and p300 and could be effective combined regimen to sensitize KRASMut NSCLC.
Structural characteristics of the moss (bryophyte) layer and its underlying soil structure and water retention characteristics
Authors: Hu Xia 
Published: March 2023

Abstract
Aims
The influence of the moss layer on soil structure and soil water retention is not well understood. Therefore, this study aims to investigate the changes in soil structure and soil water retention under moss layer and to reveal the influencing factors of these changes.
Methods
3D networks of soil macropores were quantified using CT scanning and image analysis techniques, and soil water retention characteristics were quantified through soil water retention curves (SWRCs) and VG model.
Results
The length density of the soil macropores under the thin moss and thick moss layers was approximately 2.7 and 1.6 times higher than that under no moss cover. The soil water retention properties under the thin and thick moss layers were better than those under no moss cover, with the highest plant-available water capacity under the thin moss layer. The plant-available water capacity of soils under the thin moss layer was higher than that of soils under the thick moss layer. The maximum water-holding capacity of the moss layer was significantly positively correlated with the field water-holding capacity of the soil and negatively correlated with the equivalent diameter of the macropores, while the storage capacity of the moss layer was significantly negatively correlated with the maximum effective water content of the soil.
Conclusions
The effect of the moss layer on water retention may be mainly through influencing the pore distribution and organic matter accumulation. The moss layer had a positive ecohydrological effect on soil water retention and even water conservation in forest soils.
Bifunctional circular DNA amplifier transforms a classic CRISPR/Cas sensor into an ultrasensitive autocatalytic sensor
Authors: Deng Fei , Sang Rui , Li Yi , Deng Wei , Goldys Ewa 
Published: March 2023

Abstract
The CRISPR/Cas sensors represent a new approach in molecular diagnostics. Based on the programmable trans-cleavage of Type V & VI Cas proteins to degrade single-strand DNA/RNA reporters they detect nucleic acids with single-nucleotide specificity. However, their clinical usefulness is limited by their pM level sensitivity, which is insufficient unless integrated with preamplification. To overcome this limitation, we designed a bifunctional circular DNA amplifier to report nucleic acid detection events and simultaneously facilitate an autocatalytic reaction network. This minimalistic design converts a classic CRISPR/Cas sensor into a DNA amplifier-enhanced CRISPR/Cas autocatalytic sensor (DANCER), without the need for additional amplification reaction or device. DANCER achieved 1 aM level (< 1 copy/uL) DNA detection (106 times improvement), within 15 mins, at room temperature, and with 11 orders of magnitude detection range. When combined with a colorimetric lateral flow assay, DANCER realized point-of-care testing of circulating tumor DNA in plasma of a colorectal cancer-mouse.
Comparative proteomics in captive giant pandas to identify proteins involved in age-related cataract formation
Authors: You Yuyan , Bai Chao , Wang Wei , Zhan Tongtong , Hu Xin , Hao Feier , Xia Maohua , Liu Yan , Ma Tao , Liu Yanhui , Zheng Changming , Pu Tianchun , Zhang Yizhuo , Lu Yanping , Ding Nan , Li Jing , Yin Yanqiang , Chen Yucun , Wang Liqin , Zhou Jun , Niu Lili , Xiu Yunfang , Lu Yan , Jia Ting , Liu Xuefeng , Zhang Chenglin 
Published: March 2023

Abstract
20% of the aged giant pandas in captivity have developed cataracts that impair their quality of life. Hardness and curvature of the panda's lens make it difficult to replicate in vitro. Therefore, early prediction will be the most effective way to prevent the occurrence of cataract in giant pandas. We carried out a quantitative proteomics analysis of 10 giant pandas to identify proteins differing in abundance between healthy and cataract-bearing animals. We identified almost 150 proteins exceeding our threshold for differential expression, most of which were associated with GO categories suggesting extracellular localization. The most significant differential abundance was associated with components of the proteasome and other proteins with a role in proteolysis or its regulation, most of which were depleted in pandas with cataracts. Other modulated proteins included components of the extracellular matrix or cytoskeleton, as well as associated signaling proteins and regulators, but we did not find any differentially expressed transcription factors. These results indicate that the formation of cataracts involves a complex network of signaling inside and outside lens cells to drive a stress response mainly at the protein level as a means to address the accumulation of protein aggregates triggered by oxidative damage. The modulated proteins also suggests that it should be possible to predict the onset of cataracts in captive pandas by taking blood samples and testing them for the presence or absence of particular protein markers.
The impact of COVID-19-related restrictions on pregnancy and abortion rates in the Republic of Georgia
Authors: Khachidze Nia , Manjavidze Tinatin , Anda Erik Eik , Nedberg Ingvild Hersoug , Sandøy Ingvild Fossgard , Rylander Charlotta 
Published: March 2023

Abstract
Background:The Republic of Georgia implemented COVID-19-related restrictions starting on 31 March 2020, when it imposed a 1-month strict lockdown, after which the country continued with some form of restrictions for 1 year. These restrictions created barriers to healthcare access, affected healthcare services, caused severe economic degradation, and changed people’s behavior. The aim of this study was to explore the impact of COVID-19-related restrictions on pregnancy and abortion rates in Georgia.
Methods: Information on pregnancy, abortion, and related variables was extracted from the Georgian Birth Registry from January 2018 through April 2022. The final study sample included 232,594 pregnancies and 86,729 abortions. We used interrupted time series analysis to study the impact of COVID-19-related restrictions.
Results: There were slightly decreasing trends in pregnancy and abortion rates in the pre-pandemic period. During the 1-month strict lockdown, pregnancy and abortion rates decreased in all investigated age groups. There were no substantial differences in pregnancy or abortion rates in the pandemic period compared to the pre-pandemic period. The precision of all estimates suggested that both small increases and decreases in pregnancy and abortion rates are reasonably compatible with our data.
Conclusions: Despite the 1-year-long COVID-19-related restrictions, our results did not indicate substantial long-term changes in pregnancy or abortion rates during the study period for any age group. This may indicate that the restrictions did not substantially influence access to contraception, abortion services, or women’s behavior.
The adaptability of new trainees in pediatrics for the current epileptic seizure classification (ILAE-2017) with a modular education program
Authors: Imanli Maharram , Simsek Erdem , Dezhakam Afshin , Kanmaz Seda , Dokurel Ipek , Serin Hepsen Mine , Yilmaz Sanem , Aktan Gul , Tekgul Hasan 
Published: March 2023

Abstract
Objective.
To evaluate the adaptability of trainees in pediatrics for the current seizure classification of the International League Against Epilepsy (ILAE-2017) with a modular education program (MEP) to provide a common language between neurologists and pediatricians.
Methods.
An MEP consisting of five modules for the current version of the ILAE-2017 seizure classification and three modules for the older ILAE-1981 version were applied to new-generation trainees in pediatrics. The MEP consisted of 50 illustrative pediatric seizure videos and an instruction manual kit including a seizure determinator. A post-test with 58 new videos was performed during the three-month follow-up period.
Results.
The overall success rate of the participants was slightly higher for ILAE-2017 than for ILAE-1981 in the post-MEP test (42% vs. 38%). Within the ILAE-2017 modules, similar success rates were defined for focal seizures (44%) and generalized seizures (39%). However, the participants more easily classified focal non-motor seizures (56.3%) than focal motor seizures (34.9%). Contrary, the participants had significantly lower correct rates for generalized non-motor seizures (26%) compared to generalized non-motor seizures (46%). The most miss-matched seizure types (> 50%) were automatisms and myoclonic seizures in focal seizures and atypical absences in generalized seizures for ILAE 2017 classification modules.
Conclusion.
The presented MEP provided considerable yields for ILAE-2017 seizure classification in pediatric trainees. However, booster applications of the MEP are needed for a better seizure classification with ILAE-2017 seizure nomenclature in clinical practice.
Diabetic nephropathy with marked extra-capillary cell proliferation: A case report
Authors: Morimoto Madoka , Namba-Hamano Tomoko , Notsu Shoki , Iwata Yukimasa , Yasuhara Yumiko , Yamato Masafumi , Isaka Yoshitaka 
Published: March 2023

Abstract
Background: Extra-capillary hypercellularity is a common finding in crescentic glomerulonephritis (GN) and focal segmental glomerulosclerosis (FSGS). In diabetic nephropathy (DN), extra-capillary hypercellularity is often observed as a finding of complications such as IgA nephropathy or microscopic polyangiitis superimposed on DN. However, in rare cases, epithelial cell proliferation may accompany DN. We experienced a case of nodular diabetic glomerulosclerosis with marked extra-capillary hypercellularity and revealed the origin of this atypical lesion using immunostainings.
Case presentation: A man in his 50s was admitted to the hospital with nephrotic syndrome, and a renal biopsy was performed. Diffuse nodular lesions and extra-capillary hypercellularity were observed, but the results of serological examination or immunofluorescent assays did not implicate any other crescentic GN. Immunostaining for claudin-1 and nephrin was performed to identify the origin of the extra-capillary lesions. Given the clinical course and pathological findings, a diagnosis of DN-associated extra-capillary cell proliferation was made.
Conclusions: Extra-capillary hypercellularity, which resembles FSGS or crescentic GN, is a rare finding in DN and should therefore be treated with caution. In such cases, co-staining for claudin-1 and nephrin may facilitate the diagnosis of DN.
DVNE-DRL: Dynamic virtual network embedding algorithm based on deep reinforcement learning
Authors: Xiao Xiancui 
Published: March 2023

Abstract
Virtual Network Embedding (VNE), as the key challenge of network resource management technology, lies in the contradiction between online embedding decision and pursuing long-term average revenue goals. Most of the previous work ignored the dynamics in Virtual Network (VN) modeling, or could not automatically detect the complex and time-varying network state to provide a reasonable network embedding scheme. In view of this, we model a network embedding framework where the topology and resource allocation change dynamically with the number of network users and workload, and then introduce a deep reinforcement learning method to solve the VNE problem. Further, a dynamic virtual network embedding algorithm based on Deep Reinforcement Learning (DRL), named DVNE-DRL, is proposed. In DVNE-DRL, VNE is modeled as Markov Decision Process (MDP), and Reinforcement Learning Agent (RLA) is used to periodically detect the state of network environment and provide a more reasonable network embedding scheme in real time. Different from the previous methods based on DRL algorithm to solve VNE problem, this paper improves the method of feature extraction and matrix optimization, and considers the characteristics of virtual network and physical network together to alleviate the problem of redundancy and slow convergence. The simulation results show that compared with the existing advanced algorithms, the acceptance rate and average revenue of DVNE-DRL are increased by about 25% and 35%, respectively.
K E Y W O R D S
VNE, DRL, embedding framework, DRL-DVNE，MDP，feature extraction