bluuebunny/crossref_metadata_2025_split huggingface dataset:
Sydnone‐Cyanines as Clickable Probes for Fluorescent Labelling
Authors: Anh Nguyen Kim , Lesur Emilie , Ribéraud Maxime , Sallustrau Antoine , Audisio Davide , Taran Frédéric 
Published: January 2023

AbstractThe synthesis of four clickable sydnone‐heptamethine cyanine derivatives is described in this article. The synthetic route is based on a palladium‐cross coupling reactions of sydnone boronates affording the desired sydnone‐cyanine conjugates in only five steps. These compounds were shown to react smoothly with cyclooctynes to form the corresponding pyrazoles clicked products quantitatively at room temperature and with rate constants up to 18 m−1 ⋅ s−1, affording interesting new tools for biorthogonal fluorescent labelling of (bio)molecules. Fluorescence properties of both sydnone‐ and pyrazole‐cyanines are described, as well.
Advancing exposure assessment approaches to improve wildlife risk assessment
Authors: Morrissey Christy , Fritsch Clémentine , Fremlin Katharine , Adams William , Borgå Katrine , Brinkmann Markus , Eulaers Igor , Gobas Frank , Moore Dwayne R. J. , van den Brink Nico , Wickwire Ted 
Published: January 2023

Abstract
The exposure assessment component of a Wildlife Ecological Risk Assessment aims to estimate the magnitude, frequency, and duration of exposure to a chemical or environmental contaminant, along with characteristics of the exposed population. This can be challenging in wildlife as there is often high uncertainty and error caused by broad-based, interspecific extrapolation and assumptions often because of a lack of data. Both the US Environmental Protection Agency (USEPA) and European Food Safety Authority (EFSA) have broadly directed exposure assessments to include estimates of the quantity (dose or concentration), frequency, and duration of exposure to a contaminant of interest while considering “all relevant factors.” This ambiguity in the inclusion or exclusion of specific factors (e.g., individual and species-specific biology, diet, or proportion time in treated or contaminated area) can significantly influence the overall risk characterization. In this review, we identify four discrete categories of complexity that should be considered in an exposure assessment—chemical, environmental, organismal, and ecological. These may require more data, but a degree of inclusion at all stages of the risk assessment is critical to moving beyond screening-level methods that have a high degree of uncertainty and suffer from conservatism and a lack of realism. We demonstrate that there are many existing and emerging scientific tools and cross-cutting solutions for tackling exposure complexity. To foster greater application of these methods in wildlife exposure assessments, we present a new framework for risk assessors to construct an “exposure matrix.” Using three case studies, we illustrate how the matrix can better inform, integrate, and more transparently communicate the important elements of complexity and realism in exposure assessments for wildlife. Modernizing wildlife exposure assessments is long overdue and will require improved collaboration, data sharing, application of standardized exposure scenarios, better communication of assumptions and uncertainty, and postregulatory tracking. Integr Environ Assess Manag 2024;20:674–698. © 2023 SETAC
Distribution of hydrophobic organic contaminants in marine sediment fines—An alternative normalization strategy?
Authors: Logemann Anna E. , Röhrs Steffen , Brockmeyer Berit 
Published: January 2023

Abstract
The necessary normalization of contaminant concentrations, in order to be able to compare contaminant content in sediments with different sediment properties, is currently not standardized within environmental monitoring and assessment programs. Therefore, this study investigates an alternative normalization strategy for hydrophobic organic contaminants (HOCs) by removing the coarse and chemically inert sediment fraction using an improved, half-automated wet-sieving method. We compare the results to commonly used TOC normalization (2.5% total organic carbon [TOC], OSPAR). Simultaneously, the study provides a comprehensive overview of HOC concentrations in sediment fines (&lt;63 µm) for the German Exclusive Economic Zone and therefore gathers information about the more bioavailable and mobile part of the sediment that particularly accumulates HOCs due to its high surface area. We analyzed bulk sediment samples and their corresponding fine grain fractions from 25 stations in the German Exclusive Economic Zone for 41 HOCs including polycyclic aromatic hydrocarbons, polychlorinated biphenyls, and organochlorine pesticides. The results indicate that the wet-sieving procedure is capable of physically normalizing the concentrations of the investigated HOCs and is useful for the comparison of concentrations in different sediment types. The wet-sieving procedure is more time consuming than the normalization to the TOC content. However, it offers the possibility of lowering the detection limits (LODs) through the analytical sample preparation procedure used, as sieving concentrates the contaminants. Therefore, a higher number of results &gt;LOD were detected in sediment fines, leading to more informative data sets. In contrast to the commonly used normalization to 2.5% TOC, the statistical analyses carried out (principal component analysis with subsequent cluster analysis) additionally indicate that physical normalization allows better differentiation of sampling sites by contaminant sources and geographic location rather than their sediment characteristics. Integr Environ Assess Manag 2023;19:1348–1360. © 2023 The Authors. Integrated Environmental Assessment and Management published by Wiley Periodicals LLC on behalf of Society of Environmental Toxicology & Chemistry (SETAC).
Is the long‐acting gonadotropin‐releasing hormone agonist long protocol better for patients with endometriosis undergoing IVF?
Authors: Wang Xiuzhu , Li Wenshu , Chen Xuemei , Zhang Wei , Chu Min , Yin Shuyuan , Chu Yongli , Liu Xuemei 
Published: January 2023

AbstractObjectiveTo investigate the effect of the long‐acting gonadotropin‐releasing hormone agonist (GnRHa) long protocol on in vitro fertilization (IVF) outcomes of patients with endometriosis (EMs).MethodsThis retrospective cohort study was carried out from July 1, 2016 to June 30, 2021. In all, 798 patients with EMs who underwent first IVF were enrolled. The patients were classified by the ovarian stimulation protocols. The clinical outcomes of IVF were compared in each group.ResultsThose EMs patients who received the long‐acting GnRHa long protocol had significantly higher clinical pregnancy rate (72.00%, 60.70% and 50.90%, respectively; P = 0.047 and 0.010) and implantation rate (51.0%, 44.6%, and 38.7%, respectively; P = 0.006 and <0.001) compared with the short‐acting GnRHa long protocol and the GnRH antagonist protocol. Live birth rate was also significantly higher than the GnRH antagonist protocol (60.10% vs. 40.0%, P = 0.032), but not statistically different from the short‐acting GnRHa (60.10% vs. 53.80%, P = 0.443). In addition, they also had significantly higher duration of stimulation, total dose of gonadotropin, and number of high‐quality embryos transferred compared with other groups (P < 0.001).ConclusionsThe long‐acting GnRHa long protocol could improve IVF outcomes of patients with EMs compared with the short‐acting GnRHa long protocol and the GnRH antagonist protocol.
Inpatient pharmacy‐driven antithrombosis stewardship efforts on warfarin to direct oral anticoagulant conversions
Authors: White Raechel T. , Bos Alexander J. , Nawarskas James J. , Rodriguez Chelsea N. , Goldberg Elle , Robinson Elisa , Hernandez Brandy , Marquez Jessica , Togami John , Burnett Allison 
Published: January 2023

AbstractBackgroundOver 6 million people in the United States require anticoagulation. Current guidelines recommend direct oral anticoagulants (DOACs) over warfarin for select indications due to increased safety and similar efficacy. However, data suggest warfarin to DOAC conversion occurs in less than 5% of patients.ObjectivesTo assess rates of conversion from warfarin to DOACs and the rate of inpatient pharmacist assessment for warfarin to DOAC conversions using an evidence‐based DOAC checklist.MethodsIn this retrospective cohort study, all patients ≥18 years old admitted on warfarin from January 1, 2018 to February 28, 2020 were identified via query of the electronic health record (EHR). Patients were excluded if discharged to hospice or if anticoagulation was permanently discontinued. In January 2019, an evidence‐based checklist was developed by the inpatient antithrombosis stewardship team and embedded into an EHR‐based standardized clinical documentation form prompting pharmacist assessment for DOAC therapy. Autoregressive integrated moving average (ARIMA) models were utilized to compare pre‐ and post‐implementation rates of pharmacist‐driven evaluation for warfarin to DOAC switch along with actual conversion rates. Additional outcomes were documented reasons for not switching and proportion of newly‐switched patients referred to our antithrombosis clinic.ResultsUltimately, 538 inpatient encounters were included (237 pre‐implementation of the DOAC eligibility checklist; 301 post‐implementation). Warfarin to DOAC conversions increased from a mean of 1.08 patients/month pre‐implementation of the DOAC eligibility checklist to 3.62 patients/month post‐implementation (p < 0.0001). Likewise, pharmacist evaluation for switch increased significantly from a mean of 2.00 patients/month pre‐implementation of the DOAC eligibility checklist to 17.69 patients/month post‐implementation (p < 0.0001). In addition, referrals to the anticoagulation clinic significantly increased from a mean of 1.08–4.08 patients/month (p < 0.0001).ConclusionUse of an evidence‐based checklist embedded into the inpatient EHR increased warfarin to DOAC conversions and supported overall antithrombosis stewardship efforts. Conversion rates remained fairly low. Further opportunity exists to optimize patient safety and health systems savings with ongoing stewardship efforts.
Enzyme‐Cleaved Bone Marrow Transplantation Improves the Engraftment of Bone Marrow Mesenchymal Stem Cells
Authors: Kawai Hotaka , Oo May Wathone , Takabatake Kiyofumi , Tosa Ikue , Soe Yamin , Eain Htoo Shwe , Sanou Sho , Fushimi Shigeko , Sukegawa Shintaro , Nakano Keisuke , Takeshi Takarada , Nagatsuka Hitoshi 
Published: January 2023

ABSTRACTMesenchymal stem cell (MSC) therapy is a promising approach to curing bone diseases and disorders. In treating genetic bone disorders, MSC therapy is local or systemic transplantation of isolated and in vitro proliferated MSC rather than bone marrow transplantation. Recent evidence showed that bone marrow MSC engraftment to bone regeneration has been controversial in animal and human studies. Here, our modified bone marrow transplantation (BMT) method solved this problem. Like routine BMT, our modified method involves three steps: (i) isolation of bone marrow cells from the donor, (ii) whole‐body lethal irradiation to the recipient, and (iii) injection of isolated bone marrow cells into irradiated recipient mice via the tail vein. The significant modification is imported at the bone marrow isolation step. While the bone marrow cells are flushed out from the bone marrow with the medium in routine BMT, we applied the enzymes’ (collagenase type 4 and dispase) integrated medium to wash out the bone marrow cells. Then, cells were incubated in enzyme integrated solution at 37°C for 10 minutes. This modification designated BMT as collagenase‐integrated BMT (c‐BMT). Notably, successful engraftment of bone marrow MSC to the new bone formation, such as osteoblasts and chondrocytes, occurs in c‐BMT mice, whereas routine BMT mice do not recruit bone marrow MSC. Indeed, flow cytometry data showed that c‐BMT includes a higher proportion of LepR+, CD51+, or RUNX2+ non‐hematopoietic cells than BMT. These findings suggested that c‐BMT is a time‐efficient and more reliable technique that ensures the disaggregation and collection of bone marrow stem cells and engraftment of bone marrow MSC to the recipient. Hence, we proposed that c‐BMT might be a promising approach to curing genetic bone disorders. © 2023 The Authors. JBMR Plus published by Wiley Periodicals LLC on behalf of American Society for Bone and Mineral Research.
Dieckol, a natural polyphenolic drug, inhibits the proliferation and migration of colon cancer cells by inhibiting PI3K, AKT, and mTOR phosphorylation
Authors: Dai Wei , Dai Yong gang , Ren Dong feng , Zhu Da wei 
Published: January 2023

AbstractThis study investigated that dieckol (DKL), a natural drug, inhibits colon cancer cell proliferation and migration by inhibiting phosphoinositide‐3‐kinase (PI3K), protein kinase B (AKT), and mammalian target of rapamycin (mTOR) phosphorylation in HCT‐116 cells. The cells were treated with DKL in various concentrations (32 and 50 μM) for 24 h and then analyzed for various experiments. MTT (tetrazolium bromide) and crystal violet assay investigated DKL‐mediated cytotoxicity. Dichlorodihydrofluorescein diacetate staining was used to assess the reactive oxygen species (ROS) measurement, and apoptotic changes were studied by dual acridine orange and ethidium bromide staining. Protein expression of cell survival, cell cycle, proliferation, and apoptosis protein was evaluated by western blot analysis. Results indicated that DKL produces significant cytotoxicity in HCT‐116, and the half‐maximal inhibitory concentration was found to be 32 μM for 24‐h incubation. Moreover, effective production of ROS and enhanced apoptotic signs were observed upon DKL treatment in HCT‐116. DKL induces the expression of phosphorylated PI3K, AKT, and mToR‐associated enhanced expression of cyclin‐D1, proliferating cell nuclear antigen, cyclin‐dependent kinase (CDK)‐4, CDK‐6, and Bcl‐2 in HCT‐116. In addition, proapoptotic proteins such as Bax, caspase‐9, and caspase‐3 were significantly enhanced by DKL treatment in HCT‐116. Hence, DKL has been considered a chemotherapeutic drug by impeding the expression of PI3K‐, AKT‐, and mTOR‐mediated inhibition of proliferation and cell cycle‐regulating proteins.
Novel fluorescent probe based on dicoumarin for detection of hydrogen sulfide in real samples
Authors: Hong Tong , Cheng Song , Zhong Xuefang , Zuo Yiwei , Dong Yiming , Shi Zhichuan , Zhao Zhigang 
Published: January 2023

AbstractA novel dicoumarin‐derived hydrogen sulfide (H2S) selective fluorescent “turn‐on” probe 3‐(2,4‐dinitrobenzenesulfonate)‐dicoumarin (DC‐HS) created by covalent bonding between the 2,4‐dinitrobenzenesulfonyl (DNBS) and the 3‐hydroxy‐dicoumarin (DC‐OH) units. Upon the addition of H2S, the probe DC‐HS solution's fluorescence significantly increased, and its appearance is changed from practically colorless to brilliant yellow. Probe DC‐HS also showed significant fluorescence amplification that was quantitatively detectable in the concentration range of 0–1.5 μM and had a low detection limit or limit of detection of 0.2 nM. Moreover, with a high recovery rate and excellent accuracy, the developed fluorescent molecule was used successfully for the analysis of H2S in red wine samples.
CuFe2O4 nanomaterials: Current discoveries in synthesis, catalytic efficiency in coupling reactions, and their environmental applications
Authors: Sharma Shaily , Jakhar Prakash , Sharma Himanshu 
Published: January 2023

AbstractIn the last few decades, there has been enormous growth in ferrite nanoparticles. Magnetic, optical, and electrical properties of ferrites gain consideration due to their use in various applications such as rechargeable lithium batteries, medical diagnostics, solar energy devices, and so forth. A vast increase in interest in ferrite nanoparticles has led them to be used as catalysts in various applications as they possess a large surface area‐to‐volume ratio. Furthermore, iron‐based magnetic characteristics make it simple to retrieve catalysts by using an external magnet. Iron's catalytic potential, however, is far less than copper's. Therefore, the catalytic scope is substantially increased by substituting copper within the crystal lattice. Recently copper ferrite nanoparticles have caught the interest of numerous researchers due to low‐cost magnetic material, stability under diverse conditions, and ease at which catalyst can be retrieved using an external magnetic field and utilized repeatedly. This review of data from year 2010 through 2022 emphasizes the synthesis method, structure, application in dyes degradation, catalytic potential in the number of coupling reactions, recyclability, and reusability of the magnetic catalyst.
Neuropathic pain, cognitive fusion, and alexithymia in patients with multiple sclerosis: Cross‐sectional evidence for an explanatory model of anxiety symptoms
Authors: Carvalho Teresa , Gomes Carolina , Rodrigues Adriana , da Motta Carolina 
Published: January 2023

AbstractMultiple sclerosis (MS) presents a high prevalence, a marked increase worldwide, and a relevant impact on patients, public health, and society. Anxiety often cooccurs with MS and can contribute to the worsening of MS symptoms. However, knowledge about predictors of anxiety in Patients with MS (PwMS) is scarce.ObjectiveThis preliminary study explored a novel model for anxiety symptoms in PwMS, including neuropathic pain (NeP), cognitive fusion (CF), experiential avoidance (EA), and alexithymia as explanatory factors.MethodThis cross‐sectional study integrated two independent convenience samples: 107 PwMS recruited from the Portuguese Society for Multiple Sclerosis and 97 age‐ and gender‐matched participants without the MS diagnosis (no‐MS sample) recruited from the Portuguese general population. Self‐report questionnaires that measured the constructs included in the model were administered to both groups.ResultsPwMS showed significantly higher values regarding anxiety symptoms and their explanatory variables (NeP, CF, EA, alexithymia) in comparison to non‐MS participants. In the MS sample, no correlations were found between anxiety symptoms and sociodemographic and clinical characteristics. NeP, CF, and alexithymia showed significant correlations with anxiety symptoms and significantly explained this symptomatology in simple linear regression models. Thus, these variables were retained in the multiple linear regression model and emerged as significant regressors that together explained 38% of the variance in anxious symptomatology in PwMS.ConclusionsThis preliminary study provides novel evidence on NeP and some maladaptive emotion regulation strategies related to EA/psychological inflexibility, as vulnerability to anxiety in PwMS can be considerably increased by CF and alexithymia. Clinical implications were discussed.
Rational design of a novel halotolerant ATP regeneration system for biocatalytic CTP production
Authors: Zhang Jie , Sun Chenlei , Wen Qingshi , Miao Rongxin , Zhang Bingyun , Yan Ziyi , Ying Hanjie , Liu Dong , Wang Junzhi 
Published: January 2023

AbstractBACKGROUNDAcetyl phosphate/acetate kinase system is a cost‐saving and efficient ATP regeneration system. However, it suffers from accumulation of salts during catalytic reactions. It inhibits the activity of acetate kinase (ACK), a crucial enzyme of this process, and hence decreases the rate of product accumulation and yield of CTP.RESULTSA new halotolerant ACK enzyme based ATP regeneration platform was established in this research by applying the concept of rational design to optimize its building blocks. Selection of residues for rational design followed several rules. First, the less conserved residue positions on the protein surface were considered. Second, avoiding significant residue interactions, suchas salt bridges. Third, following the feature of nature occurring halotolerant enzymes, the basic and neutral surface residues were replaced with acidic ones. Our mutants represent high catalytic activities and high IC50 value (inhibit activity by 50%) at high salt concentrations. Also, when the halotolerant ACK was applied to catalyze CTP production, the maximum titer was 213.5 ± 1.6 mM (which is 12.3‐fold higher than that of the traditional process), the productivity was 13.3 ± 0.1 mM L−1 h−1, and the molar yield to 5′‐cytidylate monophosphate (CMP) and utilization efficiency of energy were 92.4% and 72.7%, respectively.CONCLUSIONThis strategy can be applied to modify other enzymes in the industry and gives insight into resolving salt accumulation during biocatalytic processes. In addition, the halotolerant Adenosine Triphosphate (ATP) regeneration system built in this work can be applied to the synthesis of several other high energy compounds. © 2023 Society of Chemical Industry (SCI).
Pilot‐scale treatment cycle comprising membrane, thermal, and adsorption processes for spent metalworking fluids remediation
Authors: Di Capua Francesco , Ferraro Alberto , Lepore Lavinia , Fratino Umberto , Piccinni Alberto Ferruccio , Spasiano Danilo 
Published: January 2023

AbstractBACKGROUNDSpent metalworking fluids (SMWFs) represent a process waste characterized by low biodegradability, high toxicity, and very heterogeneous contamination typology connoted by the occurrence of numerous different pollutants. In this regard, the development of technically flexible and efficient treatment systems is fundamental to prevent sanitary and environmental risks deriving from SMWF disposal.RESULTSA treatment cycle including ultrafiltration (UF), evaporation, reverse osmosis (RO), and granular activated carbon (GAC) adsorption was tested at pilot scale for the remediation of three real SMWFs. A total of 44 parameters (physical, chemical, and ecotoxicological), identifying the SMWF characteristics and contamination, were analyzed after each treatment unit. The treatment cycle performance was investigated with and without the UF phase. In the complete cycle, UF was effective for the decrease of (average values) total suspended solids (96%), total hydrocarbons (>99%), animal fats and oils (>99%), and ecotoxicity (85%). In the same cycle, the evaporation significantly reduced the organic content (91% on average), and RO followed by GAC adsorption further provided effluents suitable for reuse in industrial applications. For the cycle without UF, parameters such as chemical oxygen demand, biochemical oxygen demand, and ecotoxicity showed values still significant (on average equal to 5550 mg L−1, 1933 mg L−1, and 95%, respectively) after evaporation. However, RO and GAC adsorption units allowed the achievement of effluents suitable for discharge in sewer systems.CONCLUSIONSAccording to the results, this work provides treatment strategies displaying high SMWF remediation efficiency and wide adaptability over various requirements for the achievement of specific effluent quality standards. © 2023 The Authors. Journal of Chemical Technology and Biotechnology published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry (SCI).