bluuebunny/crossref_metadata_2025_split huggingface dataset:
PENGARUH POSTINGAN AKUN INSTAGRAM @ABOUTTNG TERHADAP PEMENUHAN KEBUTUHAN INFORMASI KULINER FOLLOWERS
Authors: Santi Endang Tri , Amelia Mela , Mulyasih Rahmi 
Published: August 2023

Penelitian ini bertujuan untuk mengetahui pengaruh postingan akun instagram @abouttng terhadap pemenuhan kebutuhan informasi kuliner followers. Penelitian ini menggunakan paradigma positivis, pendekatan kuantitatif, dan menggunakan teori uses and gratification. Populasi dalam penelitian ini berjumlah 100 responden, dengan teknik pengambilan sampel menggunakan metode Simple Random Sampling yaitu pemilihan sampel secara acak, dimana setiap anggota populasi memiliki kesempatan yang sama untuk terpilih menjadi sampel. Data penelitian diperoleh dengan teknik kuesioner menggunakan skala ordinal sedangkan data pendukung didapat dari kepustakaan. Hasil penelitian ini menunjukan bahwa postingan akun instagram @abouttng memiliki pengaruh terhadap pemenuhan kebutuhan informasi kuliner followers. Hasil perhitungan nilai RSquare sebesar 0,675 menjelaskan bahwa 45% akun instagram @abouttng berpengaruh terhadap pemenuhan kebutuhan informasi kuliner sedangkan 55% sisanya dipengaruhi oleh variable lain yang tidak diteliti dalam variable ini. Hasil uji hipotesis menunjukan bahwa terdapat pengaruh yang signifikan terhadap postingan akun Instagram terhadap pemenuhan kebutuhan informasi kuliner followers.
Kata Kunci: Teori Uses and Grafitication, Instagram, Kebutuhan Informasi
PERAN KEPEMIMPINAN MINANGKABAU UNIVERSAL DALAM PENINGKATAN KINERJA USAHA RINTISAN GENERASI MILLENIAL DI KOTA PADANG
Authors: Andriani Chichi , Thaib Ilham 
Published: August 2023

Penelitian ini bertujuan untuk mengetahui peran Kepemimpinan Minangkabau Universal terhadap kinerja usaha rintisan generasi millennial di kota Padang. Pendekatan yang digunakan dalam penelitian ini adalah pendekatan deskriptif kuantitatif. Adapun objek objek dari penelitian adalah seluruh usaha rintisan generasi millenial yang berada di kota Padang, dengan populasi seluruh seluruh coffee shop yang berada di kota Padang. Teknik pengambilan sampel menggunakan teknik purposive sampling, sehingga sampel penelitian ini adalah adalah sebanyak 180 coffee shop yang ada di kota Padang. Jenis data yang digunakan adalah data primer dengan metode pengumpulan data kuesioner. Teknik analisis data dalam penelitian ini adalah menggunakan analisis regresi sederhana dengan semua data telah memenuhi uji asumsi klasik, uji validitas dan realibilitas. Dari hasil penelitian diperoleh bahwa Kepemimpinan Minangkabau Universal memberikan peran dan pengaruh yang positif dan signifikan terhadap kinerja usaha rintisan generasi millennial di kota Padang.
Kata Kunci: Kepemimpinan Minangkabau Universal, Kinerja
Identification of hub genes and key signaling pathways by weighted gene co-expression network analysis for human aortic stenosis and insufficiency
Authors: Yang Yang , Xiao Bing , Feng Xin , Chen Yue , Wang Qunhui , Fang Jing , Zhou Ping , Wei Xiang , Cheng Lin 
Published: August 2023

BackgroundHuman aortic valve stenosis (AS) and insufficiency (AI) are common diseases in aging population. Identifying the molecular regulatory networks of AS and AI is expected to offer novel perspectives for AS and AI treatment.MethodsHighly correlated modules with the progression of AS and AI were identified by weighted genes co-expression network analysis (WGCNA). Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses were performed by the clusterProfiler program package. Differentially expressed genes (DEGs) were identified by the DESeqDataSetFromMatrix function of the DESeq2 program package. The protein-protein interaction (PPI) network analyses were implemented using the STRING online tool and visualized with Cytoscape software. The DEGs in AS and AI groups were overlapped with the top 30 genes with highest connectivity to screen out ten hub genes. The ten hub genes were verified by analyzing the data in high throughput RNA-sequencing dataset and real-time PCR assay using AS and AI aortic valve samples.ResultsBy WGCNA algorithm, 302 highly correlated genes with the degree of AS, degree of AI, and heart failure were identified from highly correlated modules. GO analyses showed that highly correlated genes had close relationship with collagen fibril organization, extracellular matrix organization and extracellular structure organization. KEGG analyses also manifested that protein digestion and absorption, and glutathione metabolism were probably involved in AS and AI pathological courses. Moreover, DEGs were picked out for 302 highly correlated genes in AS and AI groups relative to the normal control group. The PPI network analyses indicated the connectivity among these highly correlated genes. Finally, ten hub genes (CD74, COL1A1, TXNRD1, CCND1, COL5A1, SERPINH1, BCL6, ITGA10, FOS, and JUNB) in AS and AI were found out and verified.ConclusionOur study may provide the underlying molecular targets for the mechanism research, diagnosis, and treatment of AS and AI in the future.
Tracking-related experiences of second-generation Asian Indian students in the USA
Authors: Banerjee Dina , Bhattacharya Akshaj Dev 
Published: August 2023

IntroductionWhile tracking is a powerful determinant of educational inequality, scholarship pays little attention to tracking-related experiences. Tracking-related experiences of students reveal their challenges and coping mechanisms. Consequently, one learns the fundamentals of the agency. This study focuses on tracking the experiences of second-generation Asian Indian students in the USA. Between educational and economic success and discrimination, Asian Indians constitute an interesting population to study tracking.MethodsData are derived from 177 in-depth interviews with participants from four sample points. And they are analyzed qualitatively using the grounded theory method.ResultsTracking-related experiences of second-generation Asian Indian students are characterized by a challenge that reflects discrimination featuring the Indian identity.DiscussionThis study extends the theories on educational inequality and racial microaggression.
Familial and genetic association with neurodevelopmental disorders caused by a heterozygous variant in the SRRM2 gene
Authors: Zhang Tao , Xu Lei , Zhu Hongdan , Ying Yuyi , Ding Jinlong , Ding Haigang , Shi Xiaoliang , He Yao , Jin Xin , Xia Guiyu 
Published: August 2023

BackgroundNeurodevelopmental disorders (NDDs) are a class of disorders affecting brain development and function, characterized by an inability to reach cognitive, emotional, and motor developmental milestones. The pathology of NDDs is complex. A recent study found that variants in the SRRM2 gene cause NDDs. However, genetic conditions play the most important role in the etiology of NDD. The genetic causes of NDD are extremely heterogeneous, leading to certain challenges in clinical diagnosis.MethodsA pregnant woman with congenital intelligence disorder came to our hospital for genetic diagnosis to predict the status of her fetus. Her mother and a brother also suffer from congenital intelligence disorder. She has a daughter with speech delay. Whole exome sequencing was used to identify a mutation (c.1415C&gt;G) in the SRRM2 gene of this family that resulted in a change in the 472nd amino acid residue of the SRRM2 protein from serine to terminated.ConclusionWe report a family with an autosomal dominant genetic disorder caused by variants in the SRRM2 gene causing NDDs. Prenatal diagnosis can help patients with this genetic disorder to have healthy offspring.
Characteristics of immunotherapy trials for nasopharyngeal carcinoma over a 15-year period
Authors: Huang Huageng , Yao Yuyi , Deng Xinyi , Weng Huawei , Chen Zegeng , Yu Le , Wang Zhao , Fang Xiaojie , Hong Huangming , Huang He , Lin Tongyu 
Published: August 2023

BackgroundImmunotherapy has been a hotspot in nasopharyngeal carcinoma (NPC) in recent years. This study aimed to provide a comprehensive landscape of the characteristics of immunotherapy clinical trials in NPC and to determine whether contemporary studies are of sufficient quality to demonstrate therapeutic value.MethodsThis is a cross-sectional analysis of NPC trials registered on ClinicalTrials.gov in the last 15 years (Jan 1, 2008-Nov 20, 2022). Only interventional trials with a primary purpose of treatment were included in the final analysis. Characteristics of immunotherapy trials were compared with those of other NPC trials. Chronological shifts in NPC immunotherapy trials were also analyzed.ResultsOf the 440 NPC studies selected, 161 (36.6%) were immunotherapy trials and 279 (63.4%) were other NPC trials. NPC immunotherapy trials were more likely than other NPC trials to be phase 1-2 (82.6% vs. 66.7%, P &lt; 0.001), single-arm (51.3% vs. 39.6%, P = 0.020), non-randomized (64.8% vs. 44.4%, P &lt; 0.001), and enroll fewer than 50 participants (46.3% vs. 34.4%, P = 0.015). Blinding was used in 8.8% of NPC immunotherapy trials. Also, 90.7% of NPC immunotherapy trials were recruited nationally and 82.6% were Asia-centric. Although academic institutions and governments (72.7%) were the major sponsors of NPC trials, immunotherapy trials were more likely to be industry-funded than other NPC trials (34.2% vs. 11.5%, P &lt; 0.001). The number of NPC immunotherapy trials increased exponentially after 2017, attributed to the exploration of immune checkpoint inhibitors. Immunotherapy combined with chemotherapy was the most commonly investigated regimen.ConclusionNPC immunotherapy trials over a 15-year period were predominantly exploratory. To generate high-quality evidence and advance the clinical application of immunotherapy in NPC, more attention and concerted efforts are needed.
Molecular bioinformatic and functional analysis of Enolase4 gene during siphonal autotomy and regeneration of razor clam Solen grandis
Authors: Zhang Zhidong , Sun Xuefeng , Chen Aihua , Yang Jiaxin , Chen Suhua , Zhang Yu , Cao Yi , Peng Yuheng , Zhu Yanqing , Wu Yangping 
Published: August 2023

For the razor clam Solen grandis, siphonal autotomy and regeneration is a necessary means of facing external stress, which is accompanied by a large amount of energy consumption. To explore the function of the ENO4 gene in the siphonal autotomy and regeneration of S. grandis, the full-length cDNA of the ENO4 in S. grandis was cloned and characterized. The full-length cDNA of SgENO4 was 2,773 base pairs (bp), with an 1,851 bp coding sequence (CDS) encoding 616 amino acids. ​Bioinformatic analysis revealed a slightly lower codon usage bias and a relatively stable nucleotide composition in the SgENO4 sequence. Three conserved motifs were found from the SgENO4 sequence. These conserved motifs vary in length from 14 to 27 amino acids. Moreover, in the siphonal pre-autotomy of razor clams, expression level of the SgENO4 gene was significantly higher in the hepatopancreas and siphonal base than other tissues (P &lt; 0.05). However, the expression of the SgENO4 gene in the siphonal base decreased significantly in the siphonal post-autotomy 7 hours and gradually recovered as the regeneration process continued. ​Linear correlation analysis showed that there was a significant linear correlation between specific weight of siphon and relative expression levels of SgENO4 (R2 = 0.826). RNA interference (RNAi) result showed that the specific weight (the proportion of siphonal weight to body weight) is significantly lower than other groups on the seventh day (P &lt; 0.05), suggesting that interfering with the expression of the SgENO4 gene could inhibit the siphonal regeneration of S. grandis. These results further demonstrate that SgENO4 is a conserved sequence and that it plays a crucial role in the siphonal autotomy and regeneration of S. grandis, advancing further understanding in exploring molecular mechanisms in the siphonal autotomy and regeneration.
Successful secukinumab therapy in plaque psoriasis is associated with altered gut microbiota and related functional changes
Authors: Du Xueshan , Yan Cong , Kong Shuzhen , Che Delu , Peng Bin , Zhu Longfei , Geng Songmei , Guo Kun 
Published: August 2023

IntroductionThe role of gut microbiome dysbiosis in the pathogenesis of psoriasis has gained increasing attention in recent years. Secukinumab, targeting interleukin (IL)-17, has a promising efficacy in psoriasis treatment. However, it remains unclear the gut microbiota alteration and related functional changes caused by successful secukinumab therapy in psoriatic patients.MethodsIn our study, we compared the fecal microbiome profile between psoriatic patients after secukinumab successful treatment (AT) and the other two groups, psoriatic patients without therapy (BT) and healthy people (H), respectively, by using next-generation sequencing targeting 16S ribosomal RNA. Then, shotgun metagenomic sequencing was first used to characterize bacterial gut microbial communities and related functional changes in the AT group.ResultsWe found that the diversity and structure of the microbial community in the AT group were significantly changed compared to those in the BT group and the H group. The AT group showed a microbiota profile characterized by increased proportions of the phylum Firmicute, families Ruminococcaceae, and a reduction in the phylum Bacteroidota (elevated F/B ratio). To detect functional alteration, we discovered that secukinumab treatment may construct a more stable homeostasis of the gut microbiome with functional alteration. There were different KEGG pathways, such as the downregulated cardiovascular diseases pathway and the upregulated infectious diseases in the AT group. By metagenomic analysis, the metabolic functional pathway was changed after secukinumab therapy.DiscussionIt seems that gut microbiota investigation during biologic drug treatment is useful for predicting the efficacy and risks of drug treatment in disease.
Objective evaluation of intracochlear electrocochleography: repeatability, thresholds, and tonotopic patterns
Authors: Schuerch Klaus , Wimmer Wilhelm , Rummel Christian , Caversaccio Marco Domenico , Weder Stefan 
Published: August 2023

IntroductionIntracochlear electrocochleography (ECochG) is increasingly being used to measure residual inner ear function in cochlear implant (CI) recipients. ECochG signals reflect the state of the inner ear and can be measured during implantation and post-operatively. The aim of our study was to apply an objective deep learning (DL)-based algorithm to assess the reproducibility of longitudinally recorded ECochG signals, compare them with audiometric hearing thresholds, and identify signal patterns and tonotopic behavior.MethodsWe used a previously published objective DL-based algorithm to evaluate post-operative intracochlear ECochG signals collected from 21 ears. The same measurement protocol was repeated three times over 3 months. Additionally, we measured the pure-tone thresholds and subjective loudness estimates for correlation with the objectively detected ECochG signals. Recordings were made on at least four electrodes at three intensity levels. We extracted the electrode positions from computed tomography (CT) scans and used this information to evaluate the tonotopic characteristics of the ECochG responses.ResultsThe objectively detected ECochG signals exhibited substantial repeatability over a 3-month period (bias-adjusted kappa, 0.68; accuracy 83.8%). Additionally, we observed a moderate-to-strong dependence of the ECochG thresholds on audiometric and subjective hearing levels. Using radiographically determined tonotopic measurement positions, we observed a tendency for tonotopic allocation with a large variance. Furthermore, maximum ECochG amplitudes exhibited a substantial basal shift. Regarding maximal amplitude patterns, most subjects exhibited a flat pattern with amplitudes evenly distributed over the electrode carrier. At higher stimulation frequencies, we observed a shift in the maximum amplitudes toward the basal turn of the cochlea.ConclusionsWe successfully implemented an objective DL-based algorithm for evaluating post-operative intracochlear ECochG recordings. We can only evaluate and compare ECochG recordings systematically and independently from experts with an objective analysis. Our results help to identify signal patterns and create a better understanding of the inner ear function with the electrode in place. In the next step, the algorithm can be applied to intra-operative measurements.
TTBK1 and CK1 inhibitors restore TDP-43 pathology and avoid disease propagation in lymphoblast from Alzheimer’s disease patients
Authors: Martinez-Gonzalez Loreto , Cuevas Eva P. , Tosat-Bitrián Carlota , Nozal Vanesa , Gil Carmen , Palomo Valle , Martín-Requero Ángeles , Martinez Ana 
Published: August 2023

IntroductionTDP-43 proteinopathy in Alzheimer’s disease (AD) patients is recently emerging as a relevant pathomolecular event that may have been overlooked. Recent results in immortalized lymphocytes from AD patients have shown not only an increase of post-translational modifications in TDP-43, such as hyperphosphorylation and fragmentation, but also its prionic behaviour and cell-to-cell disease transmission. With the main goal to advance therapeutic interventions, we present in this work different kinase inhibitors with potential to restore this pathological mechanism.MethodologyWe have used immortalized lymphocytes from healthy controls and AD severe patients to evaluate the correction of TDP-43 pathology after the treatment with previously synthetized TTBK1 and CK1 inhibitors. Moreover we used the conditioned mediums of these cells to perform different disease propagation experiments.ResultsTDP-43 pathology observed in lymphoblasts from severe AD patients is reduced after the treatment with TTBK1 and CK1 inhibitors (decreasing phosphorylation and increasing nuclear localisation), Furthermore, the significant increase in TDP-43 phosphorylation, cytoplasmic accumulation and aberrant F-actin protrusions (TNT-like structures) observed in control cells growing in CM from AD lymphoblasts were abolished when the CM from AD lymphoblasts treated with previously reported TTBK1 and CK1 inhibitors were used. In addition, the cytosolic transport mediated by molecular motors of the receptor cells was altered with the induced TDP-43 pathology, but it was not produced with the abovementioned pretreated CMs.ConclusionTTBK1 and CK1 inhibitors, specially VNG1.47 and IGS2.7 compounds, restore TDP-43 pathology and avoid cell-to-cell propagation in immortalized lymphocytes from AD patients, being excellent candidates for the future therapy of this prevalent and devastating disease.
Molecular characteristics, clinical significance, and immune landscape of extracellular matrix remodeling-associated genes in colorectal cancer
Authors: Chen Wenlong , Wang Yiwen , Gu Haitao , Zhang Yi , Chen Cong , Yu Tingting , Chen Tao 
Published: August 2023

BackgroundExtracellular matrix (ECM) remodeling is one of the hallmark events in cancer and has been shown to be closely related to tumor immunity. Immunotherapy has evolved as an important tool to treat various cancers and improve patient prognosis. The positive response to immunotherapy relies on the unique interaction between cancer and the tumor microenvironment (TME). However, the relationship between ECM remodeling and clinical outcomes, immune cell infiltration, and immunotherapy in colorectal cancer (CRC) remains unknown.MethodsWe systematically evaluated 69 ECM remodeling-associated genes (EAGs) and comprehensively identified interactions between ECM remodeling and prognosis and the immune microenvironment in CRC patients. The EAG_score was used to quantify the subtype of ECM remodeling in patients. We then assessed their value in predicting prognosis and responding to treatment in CRC.ResultsAfter elaborating the molecular characteristics of ECM remodeling-related genes in CRC patients, a model consisting of two ECM remodeling-related genes (MEIS2, SLC2A3) was developed for predicting the prognosis of CRC patients, Receiver Operating Characteristic (ROC) and Kaplan-Meier (K-M) analysis verified its reliable predictive ability. Furthermore, we created a highly reliable nomogram to enhance the clinical feasibility of the EAG_score. Significantly differences in TME and immune function, such as macrophages and CD8+ T cells, were observed between high- and low-risk CRC patients. In addition, drug sensitivity is also strongly related to EAG_score.ConclusionOverall, we developed a prognostic model associated with ECM remodeling, provided meaningful clinical implications for immunotherapy, and facilitated individualized treatment for CRC patients. Further studies are needed to reveal the underlying mechanisms of ECM remodeling in CRC.
Treatment perspective after failed open reduction of congenital hip dislocation. A systematic review
Authors: Merckaert Sophie , Zambelli Pierre-Yves 
Published: August 2023

BackgroundFailure of open reduction of developmental hip dislocation is a serious complication and revision surgery appear to be technically demanding with high complication rates. Little attention has been given in literature to patients in whom open reduction of developmental hip dislocation has failed. We present a systematic review about current perspectives and timing when to perform surgical revision after failed open reduction of developmental hip dislocation in children.MethodsFollowing the recommendations of the “Preferred Reporting Items for Systematic Reviews and Meta-Analyses” (PRISMA) statements we performed a comprehensive search of the PubMed and Google Scholar bibliographic database in order to select all studies published between 1980 and 2022. Studies were screened for the reasons for failure of open reduction, timing when revision surgery was performed, and for the surgical techniques used for revision.ResultsA total of 10 articles including 252 patients and 268 hips has been recorded. The most common causes of re-dislocation after open reduction are inadequate exposure and failure to release the obstructing soft tissues inside and around the hip. In 90% of the cases the anterolateral approach was performed for revision surgery. Avascular necrosis occurred in 5%–67% of cases and was the most encountered complication.ConclusionRedislocation of developmental hip dislocation after an open reduction has poor long-term outcomes mainly due to a high rate of avascular necrosis of the femoral head. It is mandatory to obtain a stable reduction at the second surgery combining soft tissue release, capsulorrhaphy, pelvic and femoral osteotomies.
Prenatal diagnosis, management, and outcomes of fetuses with tetralogy of Fallot in China after prenatal counseling: a prospective cohort study
Authors: Deng Deng-pu , Qian Tao , Liu Yu-hong , Wu Zhong-shi , Lu Ting 
Published: August 2023

ObjectiveThe study aimed to monitor fetuses with tetralogy of Fallot (TOF) after prenatal counseling and how it influenced the decision of parents to terminate the pregnancy.MethodsFetuses with isolated TOF diagnosed between January 2019 and December 2021 were prospectively enrolled. The follow-up period extended until termination or 6 months after the operation.ResultsOf the 1,026 fetuses diagnosed with cardiac defects, 129 were identified to have isolated TOF and completed the follow-up. A total of 55 (42.6%) fetuses were terminated, with larger maternal age (odds ratio: 0.893, 95% confidence interval: 0.806–0.989, P = 0.031) as the protective factor. The maternal anxiety score, gestational weeks, and pulmonary-to-aortic-diameter ratio lost significance in multivariate analysis. Subjectively, the two most common reasons for terminating the pregnancy were worries about the prognosis (41.8%) and concerns about the possible suffering of the unborn child (18.2%). The prenatal diagnosis was accurate in 73 of the 74 (98.6%) live births. Out of the 64 live births that underwent surgical repair in our center, 57 (89.1%) received primary repair, with a median age of 104 days, and 49 (76.6%) underwent valve-sparing repair. No perioperative death occurred.ConclusionsTermination for fetuses with TOF remains common in China. Live births with TOF can be safely and effectively managed.