bluuebunny/crossref_metadata_2025_split huggingface dataset:
Ginsenoside CK inhibits androgenetic alopecia by regulating Wnt/β‐catenin and p53 signaling pathways in AGA mice
Authors: Fang Chao , Yang Haixia , Zhu Chenhui , Fan Daidi , Deng Jianjun 
Published: June 2023

AbstractAndrogenetic alopecia (AGA) is one of the most common chronic skin diseases caused by the destruction of androgens in the body. It is caused by the excessive deposition of dihydrotestosterone around the hair follicle, resulting in hair follicle atrophy and hair cell apoptosis. It causes great anxiety and psychological distress to patients and affects sexual function in severe cases. As traditional Chinese herbs, ginsenosides have been proven to possess various pharmacological activities. Ginsenoside CK is one of the main ginsenosides, but its role and molecular mechanism in treating AGA remain unclear. Here, we found that ginsenoside CK intervention significantly reduced back hair loss in AGA mice, improved sexual organ damage and hair follicle cell apoptosis, and promoted hair growth in AGA mice. Ginsenoside CK treatment inhibited p53 expression, reduced the apoptosis of hair follicle cells induced by hormone deposition, activated the Wnt/β‐catenin cell proliferation signaling pathway, increased the level of vascular endothelial growth factor, promoted cell proliferation, and improved the atrophy of hair follicle tissue. Ginsenoside CK treatment also reduced testosterone levels in AGA mice, improved testicular tissue inflammation, and restored normal hormone metabolism in AGA mice. Our results indicated that ginsenoside CK reduced hair follicle cell apoptosis, promoted proliferation and development, and prolonged the hair growth cycle by regulating the Wnt and p53 signaling pathways, indicating the potential value of ginsenoside CK as a natural therapeutic agent for AGA.
Soybean‐derived peptide protects against an E. coli‐induced sepsis in cynomolgus monkeys
Authors: Liu Wei , Xu Zhenzhen , Ren Zhengyu , Amakye William Kwame , Xu Yongzhao , Gao Li , Wang Min , Ren Jiaoyan 
Published: June 2023

AbstractSepsis is a life‐threatening condition that requires urgent medical attention. In this study, we investigated the potential of soybean‐derived peptides (soy peptides) as an anti‐inflammatory supplement for patients with sepsis. Cynomolgus monkeys were induced with sepsis by intravenous administration of three different concentrations of Escherichia coli (6.3 × 103, 6.3 × 105, and 6.3 × 107 CFU/kg). Changes in body weight and inflammatory markers were analyzed, and the high dose of 6.3 × 107 CFU/kg was found to be the most appropriate for sepsis modeling. Controlled enzymatic hydrolysis was then used to release bioactive soy peptides from soy protein isolate, which were administered to monkeys with sepsis. The results showed that soy peptide supplementation significantly suppressed inflammatory responses and improved coagulation abnormalities in the monkeys. Our findings suggest that soy peptides could be an effective clinical nutritional supplement for patients with sepsis, with potential benefits for reducing inflammation and improving coagulation. These results provide a foundation for further research into the development of novel nutritional supplements for patients with sepsis.
Clinical and genomic characterization of an ATRA‐insensitive acute promyelocytic leukemia variant with a FNDC3B::RARB fusion
Authors: Kirkham Justin K. , Liu Yen‐Chun , Foy Scott G. , Ma Jing , Gheorghe Gabriela , Furtado Larissa V. , Popescu Marcela I. , Klco Jeffery M. , Karol Seth E. , Blackburn Patrick R. 
Published: June 2023

AbstractThe promyelocytic leukemia‐retinoic acid receptor‐α (PML::RARA) fusion is the hallmark of acute promyelocytic leukemia (APL) and is observed in over 95% of APL cases. RARA and homologous receptors RARB and RARG are occasionally fused to other gene partners, which differentially affect sensitivity to targeted therapies. Most APLs without RARA fusions have rearrangements involving RARG or RARB, both of which frequently show resistance to all‐trans‐retinoic acid (ATRA) and/or multiagent chemotherapy for acute myeloid leukemia (AML). We present a 13‐year‐old male diagnosed with variant APL with a novel FNDC3B::RARB in‐frame fusion that showed no response to ATRA but responded well to conventional AML therapy. While FNDC3B has been identified as a rare RARA translocation partner in ATRA‐sensitive variant APL, it has never been reported as a fusion partner with RARB and it is only the second known fusion partner with RARB in variant APL. We also show that this novel fusion confers an RNA expression signature that is similar to APL, despite clinical resistance to ATRA monotherapy.
A novel STRN3::PRKD1 fusion in a cribriform adenocarcinoma of salivary gland with high‐grade transformation
Authors: Owosho Adepitan A. , Baker Emily , Wood C. Burton , Jain Richa 
Published: June 2023

AbstractCribriform adenocarcinoma of salivary gland (CASG) is a rare form of salivary gland neoplasm that mostly arises from minor salivary glands. We report a case of CASG with high‐grade transformation harboring a novel STRN3::PRKD1 fusion. A 59‐year‐old male presented with a palatal mass. Morphologically, the tumor consisted of two components: solid high‐grade and glandular low‐grade areas. The solid high‐grade area comprised solid nests of high‐grade carcinoma with central necrosis arranged in lobules delineated with prominent stromal septa. The glandular low‐grade area comprised of cribriform and microcystic architecture in a hyalinized and hypocellular stroma. Immunophenotypically, the tumor was positive for S100 but negative for p40 and actin. However, due to the high‐grade component, tissue was sent for salivary gland NGS fusion panel analysis to confirm the diagnosis. The current case illustrates high‐grade transformation in CASG. Furthermore, identification of a STRN3::PRKD1 fusion expands the genetic spectrum of CASG.
Expression and subcellular localization of mitochondrial docking protein, syntaphilin, in oligodendrocytes and CNS myelin sheath
Authors: Nakamura Diane S. , Gothié Jean‐David M. , Kornfeld Samantha F. , Kothary Rashmi , Kennedy Timothy E. 
Published: June 2023

AbstractOligodendrocytes produce lipid‐rich myelin sheaths that provide metabolic support to the underlying axon and facilitate saltatory conduction. Oligodendrocyte mitochondria supply the bulk of energy and carbon‐chain backbones required for lipid synthesis. The sparsity of mitochondria in the myelin sheath suggests that tight regulation of mitochondrial trafficking is crucial for their efficient distribution in the cell. In particular, retention of mitochondria at axoglial junctions would support local lipid synthesis and membrane remodeling during myelination. How mitochondrial docking in oligodendrocytes is regulated is not known. Our findings indicate that syntaphilin (SNPH), a mitochondrial docking protein that has been characterized in neurons, is expressed by oligodendrocyte precursor cells (OPCs) and mature oligodendrocytes in vitro and present in the myelin sheath in vivo. We have previously reported that bath application of netrin‐1 promotes the elaboration of myelin basic protein‐positive membranes, and that localized presentation of a netrin‐1 coated microbead results in rapid accumulation of mitochondria at the site of oligodendrocyte‐bead adhesion. Here we show that netrin‐1 increases the redistribution of SNPH to oligodendrocyte processes during the expansion of myelin basic protein‐positive membranes and that SNPH clusters at the oligodendrocyte plasma membrane at sites of adhesion with netrin‐1‐coated beads where mitochondria are retained. These findings suggest roles for SNPH in oligodendrocytes regulating netrin‐1‐mediated mitochondrial docking and myelin membrane expansion.
Research on risk assessment model of subjective and objective integration in manual handling work
Authors: Ren Bin , Zhou Qinyu , Chen Jiayu 
Published: June 2023

AbstractThe main hazards in the process of manual handling work are triggered by human factors and ergonomics. This study is intended to implement a valid approach to quantitatively evaluate the risk level during manual handling work. A risk assessment model for manual handling workers was proposed based on subjective and objective correlation. A simulation experiment of manual handling process was developed and an ergonomics evaluation method was carried out. The 33‐point human joint model of BlazePose neural network and the Rapid Upper Limb Analysis (RULA) method were utilized to determine the risk posture and risk index of the manual handling workers. This study brought together hemodynamic parameters and the score of Borg Rating of Perceived Exertion (RPE) scale and Physical Resources Scale (PRS) to obtain the final comprehensive index of work risk. The results showed that the risk indexes from the three stages of the experiment obtained by RULA method were 3, 4, and 7. And the scores of comprehensive indexes obtained by the risk assessment model were 1.841, 1.900, and 1.987, suggesting that the evaluation model based on subjective and objective correlation had the same ability to determine the risk levels of different handling tasks. Therefore, the risk assessment model proposed in this study verified the effectiveness of the comprehensive evaluation index integrating hemodynamic parameters and subjective evaluation scores.
Evaluating risk factors in relation to preschool social–emotional skills using a person‐centred approach
Authors: Farrell Carmen Brown , Gilpin Ansley T. , Thibodeau‐Nielsen Rachel , Taylor Samantha Lee 
Published: June 2023

AbstractFew studies have utilised a person‐centred approach when it comes to understanding risk factors in young children. The present study uses this type of approach while examining risk factors in relation to social–emotional skills. Parent reports for 444 preschool‐aged children disclosed each child's exposure to eight different risk factors, as well as their social–emotional abilities. A latent class analysis was utilised to examine subgroups of risk, rather than using a cumulative approach, to see whether specific risk factors differentiated participants. Subgroup differences in distal social–emotional skills were then examined. The subgroup with systemic, economic and parent stress risk factors showed the lowest social–emotional abilities, in comparison to subgroups with only systemic or a combination of systemic and economic risks. These results suggest that addressing parent stress may be particularly relevant when considering children's social–emotional development. Implications for interventions and clinical practice are discussed.
Protecting pregnant women from climate disasters: Strategies in the aftermath of Pakistan's devastating flood
Authors: Zaigham Mehreen , Bryce‐Alberti Mayte , Campos Letícia Nunes , Forbes Callum , Naus Abbie E. , Pigeolet Manon , Hill Sarah K. , Sana Hamaiyal , Ehsan Anam N. , Samad Lubna , Uribe‐Leitz Tarsicio , McClain Craig D. , Juran Sabrina 
Published: June 2023

SynopsisSudden‐onset climate events can have a significant impact on maternal health care systems, particularly in low‐ and middle‐income countries where resources are limited. We outline strategic policies that can help anticipate and plan for such disasters and help minimize negative maternal outcomes.
Association of follicle‐stimulating hormone with bone turnover markers and bone mineral density in Chinese women across the menopausal transition
Authors: Li Lin , Pi Yin‐Zhen , Zhang Hong , Dai Ru‐Chun , Yuan Ling‐Qing , Sheng Zhi‐Feng , Wu Xi‐Yu 
Published: June 2023

AbstractBackgroundElevated follicle‐stimulating hormone (FSH) is associated with an increased risk of postmenopausal osteoporosis. This study investigated the association of serum FSH with bone turnover markers (BTMs) and bone mineral density (BMD) in healthy women undergoing menopausal transition.MethodsA total of 487 healthy women (age 35–65 years, 50 ± 8.5 years) were enrolled in this study. Serum FSH, BTMs, and BMD at lumbar spine and total hip were measured in these subjects.ResultsFollicle‐stimulating hormone was positively correlated with various BTMs (r = 0.339–0.583, all p < 0.001) and negatively correlated with lumbar spine and total hip BMD (r = −0.629 and −0.514, all p < 0.001). After adjusting for age and body mass index, the partial correlation coefficients of FSH with BTMs and BMD remained significant. Estimating from the regression equation, for every 10 IU/L increase in serum FSH, BTMs increased by 0.38–3.6 units, and BMD decreased by 0.03–0.05 g/cm2, respectively. Multiple linear regression analysis showed that FSH was a positive factor for serum bone‐specific alkaline phosphatase, osteocalcin, and N‐telopeptide of collagen type 1 (β = 0.188–0.403, all p < 0.001), and a negative factor for lumbar spine BMD and serum C‐telopeptide of collagen type 1 (β = −0.629 and –0.183, all p < 0.001).ConclusionsThis study suggests that serum FSH levels are an independent risk factor for BTMs and BMD in menopause‐transitioning women, particularly for serum BAP and lumbar spine BMD.
Genetic evaluation of 50 Turkish patients with neurofibromatosis type 1: 2 years experience of a single center
Authors: Kocabey Mehmet , Özkalaycı Hande , Çankaya Tufan , Yılmaz Uzman Ceren , Çağlayan Ahmet Okay , Ülgenalp Ayfer , Erçal Murat Derya 
Published: June 2023

AbstractBackground/aimNeurofibromatosis type 1 is an autosomal dominant neurocutaneous disorder. Clinical diagnosis is difficult in early childhood, and it is possible to miss a critical interval for tumour screening. In this study, we aimed to characterize the mutational spectrum of Turkish patients and discuss the benefits of molecular testing.Material and methodsFifty individuals from 35 unrelated families were included. Main referral reasons for genetic testing were as follows: to confirm a clinical diagnosis, to use in differential diagnosis and to evaluate first‐degree family member of a known patient. Two‐step process consisting of initial next generation sequencing of the NF1 gene and consequent multiplex ligation‐dependent probe amplification were performed.ResultsWe identified a total of 30 variants in 28 individuals. Variant detection rate was 56% in the entire study group and 71.4% within the index patients. Four novel variants were found. Truncating variants constituted 60% of the entire mutation spectrum. A deletion or duplication was not detected. The most common feature was cafe au lait macules in 70% of the patients, followed by focal areas of signal intensity on brain imaging (26%), cutaneous neurofibromas (24%) and axillary freckling (24%).ConclusionsEarly sequencing in all suspected patients followed by deletion/duplication analysis in patients meeting clinical criteria and a case‐to‐case based consideration for RNA studies seems to be the effective algorithm for NF‐1 diagnosis.
Administration of combined venetoclax and azacitidine in a patient with acute myeloid leukemia and multiple comorbidities undergoing dialysis: A case report
Authors: Akagi Yuina , Yamashita Yusuke , Kosako Hideki , Furuya Yoshiaki , Hosoi Hiroki , Mushino Toshiki , Murata Shogo , Nishikawa Akinori , Tamura Shinobu , Nakao Taisei , Sonoki Takashi 
Published: June 2023

AbstractPatients with acute myeloid leukemia (AML) who have comorbidities have limited treatment options, thereby resulting in poor prognosis. Venetoclax, a specific B‐cell lymphoma‐2 inhibitor, has recently been approved for AML in combination with hypomethylating agents; however, only one report has described its use in patients undergoing dialysis. Herein, we report the effectiveness of combined venetoclax and azacitidine in a 73‐year‐old man with AML undergoing dialysis and who was ineligible for standard therapies. The safety of venetoclax and azacitidine in patients undergoing dialysis has been reported, and their combination may be a feasible option for patients with AML undergoing dialysis.
Respiratory syncytial virus surge in 2022 caused by lineages already present before the COVID‐19 pandemic
Authors: Redlberger‐Fritz Monika , Springer David N. , Aberle Stephan W. , Camp Jeremy V. , Aberle Judith H. 
Published: June 2023

AbstractIn 2022, Austria experienced a severe respiratory syncytial virus (RSV) epidemic with an earlier‐than‐usual start (Weeks 35/2021–45/2022) and increased numbers of pediatric patients in emergency departments. This surge came 2 years after a season with no cases detected as a result of coronavirus disease 2019 nonpharmaceutical interventions. We analyzed epidemiologic patterns and the phylodynamics of RSV based on approximately 30 800 respiratory specimens collected year‐round over 10 years from ambulatory and hospitalized patients from 248 locations in Austria. Genomic surveillance and phylogenetic analysis of 186 RSV‐A and 187 RSV‐B partial glycoprotein sequences collected from 2018 to 2022 revealed that the 2022/2023 surge was driven by RSV‐B in contrast to the surge in the 2021/2022 season that was driven by RSV‐A. Whole‐genome sequencing and phylodynamic analysis indicated that the RSV‐B strain GB5.0.6a was the predominant genotype in the 2022/2023 season and emerged in late 2019. The results provide insight into RSV evolution and epidemiology that will be applicable to future monitoring efforts with the advent of novel vaccines and therapeutics.
Effects of nisin on bacterial community and fermentation profiles, in vitro rumen fermentation, microbiota, and methane emission of alfalfa silage
Authors: Li Ziqian , Wang Musen , Usman Samaila , Khan Ashiq , Zhang Yixin , Li Fuhou , Bai Jie , Chen Mengyan , Zhang Ying , Guo Xusheng 
Published: June 2023

AbstractBACKGROUNDAlfalfa (Medicago sativa L.) has been used widely in preparing silage. However, forage legumes are prone to contamination by spoilage bacteria during fermentation. Nisin has broad‐spectrum antibacterial properties and has been applied as an inhibitor of rumen methane emissions. However, little research has been carried out on the application of nisin in silage. This study therefore aimed to investigate the impacts of different nisin concentrations on the bacterial community and fermentation dynamics, in vitro ruminal fermentation characteristics, microbiota, and methane emissions of alfalfa silage.RESULTSThe detection limits of organic acid in nisin‐treated silages were not reached in 0.09 g kg−1 nisin (0.09 level) from days 1 to 7 of ensiling. With increasing nisin concentrations, the silage dry matter increased linearly (P < 0.05), and dry matter loss decreased linearly (P < 0.05). Moreover, both the 0.06 g kg−1 nisin (0.06 level) and 0.09 level treatments increased the relative abundance of Pediococcus acidilactici during ensiling. Concurrently, as the nisin concentrations increased, ruminal methane production decreased linearly (P < 0.05), while the relative abundances of ruminal Succinivibrio, Fibrobacter succinogenes and Ruminobacter amylophilus increased linearly (P < 0.05). The populations of ruminal total bacteria, methanogens, protozoa, and fungi decreased linearly with increasing nisin concentrations (P < 0.05).CONCLUSIONThe addition of nisin delayed the fermentation process, preserved more nutrients in alfalfa silage, and promoted fermentation dominated by P. acidilactici in the late phase of ensiling. Moreover, nisin reduced in vitro rumen methane emissions without adverse effects on dry matter digestibility. © 2023 Society of Chemical Industry.
Effect of Lactobacillus plantarum fermentation on the physicochemical properties and flavor of rice protein–carboxymethylcellulose complexes
Authors: Yang Jing , Xiong Wenfei , Yao Yijun , Zhang Na , Wang Lifeng 
Published: June 2023

AbstractBACKGROUNDFermentation is known to enhance the nutritional profile and confer unique flavors to products. However, the resultant effects on stability and physicochemical properties remain unexplored.RESULTSThis study aims to elucidate the influence of fermentation on the stability and organoleptic characteristics of a rice protein beverage stabilized by carboxymethyl cellulose (CMC). The findings revealed that the average aggregate size escalated from 507 to 870 nm, concurrently exhibiting a significant increase in surface potential. The aggregation enhancement was substantiated by evident morphological changes and confocal laser scanning microscopical (CLSM) observations. A negative correlation was discerned between the physical stability of the beverage and fermentation duration. Moreover, flavor analysis of the beverage post a 3 h fermentation period highlighted an increase in aromatic ester compounds, thereby intensifying the aroma.CONCLUSIONThe study corroborates that fermentation can detrimentally influence product stability while concurrently improving its flavor profile. By establishing a mix ratio of 10:1 for rice protein and CMC and forming a relatively stable system through electrostatic interaction at a pH of 5.4, a flavorful rice protein beverage can be derived post 3 h‐fermentation process. These findings offer insights into the impact of varying fermentation durations on the stability and flavor of polysaccharide‐based rice protein beverages. © 2023 Society of Chemical Industry.
The impact of glycerol monostearate's similarity to fats and fatty acid composition of fats on fat crystallization, destabilization, and texture properties of ice cream
Authors: Zhao Mengdi , Chen Ling , Liu Fei , Zhong Fang , Chen Maoshen , Jin Huajin , Kang Jingran , Wu Juan , Xu Jun 
Published: June 2023

AbstractBACKGROUNDFat significantly affects the properties of ice cream. Prior studies have investigated the correlation between fat crystallization, fat destabilization, and ice cream quality. However, the role of fatty acid composition, the similarity between fat and emulsifier in these characteristics, and their impact on final product quality remains unclear.RESULTSTo investigate the influence of the fatty acid composition of fats, as well as their similarity to glycerol monostearate (GMS), on fat crystallization and destabilization during the aging and freezing stages, ice creams were formulated using a combination of two types of fats (coconut oil and palm olein) in five different ratios. In oil phases, decreased saturation of fatty acids (from 93.38% to 46.69%) and increased similarity to GMS (from 11.96% to 46.01%) caused a reduction in the maximum solid fat content. Moreover, the rise in unsaturated long‐chain fatty acids (from 34.61% to 99.57%) and similarity to GMS enhanced the formation of rare and coarse fat crystals, leading to a sparse crystalline network. This, in turn, reduced the crystallization rate and the stiffness of the fat in emulsions. Assuming consistent overrun across all ice creams, the enhanced interactions between fat globules in ice cream improved its hardness, melting properties, and shrinkage.CONCLUSIONThe crystalline properties of fat in emulsions were influenced by oil phases, impacting fat destabilization and ultimately enhancing the quality of ice cream. The present study offers valuable insights for the optimization of fat and monoglyceride fatty acid ester selection, with the potential to improve ice cream quality. © 2023 Society of Chemical Industry.