bluuebunny/crossref_metadata_2025_split huggingface dataset:
Machine learning for the prediction of the in-hospital mortality of post-cardiac arrest patients ：a retrospective observational study
Authors: Lin Qingting , Zhang Nan , Jiang Hui , Zhu Huadong 
Published: July 2023

Abstract
Background
Worldwide, cardiac arrest is highly prevalent and associated with a high mortality rate. Despite timely CPR, a substantial proportion of cardiac arrest deaths occur in patients who return to spontaneous circulation (ROSC).Therefore, the purpose of this study was to explore the relevant factors affecting the prognosis of patients with cardiac arrest and develop an accurate and fast prognostic prediction model through machine learning with convenient clinical information.
Methods
We conducted a retrospective observational study. Data from 1772 cardiac arrest patients above 18 years of age from the MIMIC database were used to develop three machine learning models, including SVM, LR, and XGBoost models, for predicting in-hospital mortality. The areas under the receiver operating characteristic curve (AUC), accuracy, precision, recall and F1 score were calculated to evaluate these models.
Results
In our study, the XGBoost algorithm outperformed the other algorithms. The accuracy, recall value, precision value and F1 score of the XGBoost algorithm were 0.762, 0.812, 0.765, and 0.788, respectively. In addition, the AUC of the XGBoost model was larger than those of the LR and SVM models (0.847 vs. 0.834 vs. 0.747, respectively). The top 10 most important features of the XGboost algorithm were lactate_min,gcs_min,temperature_max,weight_kg,CK_MB_max,bun_min,glucose_min,spo2_min,wbc_min,and heart_rate_min. The XGBoost algorithm provided more personalized and reliable prognostic information for cardiac arrest patients than the other algorithms.
Conclusions
The prognostic prediction model for patients with cardiac arrest established by the XGBoost algorithm includes indicators that had certain predictive value for disease severity in previous studies. Compared with other models, this model can provide more accurate and considerable prognostic information, facilitate communication between patients' families and doctors about the disease, and help doctors make clinical decisions.
6-Gingerol Promotes Apoptosis of Cancer Cells in Ovary Through the Activation of the Signaling Pathway for miR-506/Gli3
Authors: Wu Hong-Hu , Xiong Jun , Jiang Hui , Li Huan , Tan Xiao-Qing , He Xiao-Ju , Cheng Xue 
Published: July 2023

Abstract
Background: It has been found using gingerol homologs that ginger plant rhizomes can potentially guarantee human health, which includes preventing and treating cancer. Here, we investigated 6-Gingerol’s anticancer activities and its mechanisms of action in cancer cells.
Methods: We used MTT assays to measure cell growth, flow cytometry to assess the proliferation, cytotoxicity, and cycle progression of cells, and western blotting to evaluate cyclin expression.
Results: In vitro, ovarian cancer cells underwent apoptosis following 6-Gingerol. 6-Gingerol suppressed GLI3 expression without affecting Bax, Bcl2, or Bcl-XL. We observed low miR-506 expression in ovarian cancer tissues, while 6-Gingerol significantly promotedits expression. miR-506 directly suppress GLI3 expression and caused apoptosis in SKOV3 cells.
Conclusions: Our results indicate that miR-5066-Gingerol upregulation could induce cancer cell apoptosis in the ovary. This study provides evidence supporting 6-Gingerol-based therapy for ovarian malignancy.
Identification of a Novel Hypoxia-Related Gene Prognostic Signature in Osteoarthritis
Authors: Hu Yibin , Zheng Yiyi 
Published: July 2023

Abstract
Background.
Osteoarthritis (OA) is a well-known joint disorder characterized by inflammation. Current evidence suggests that immune cell infiltration plays an important role in the development of OA. This study explored the heterogeneity of immune cell infiltration in OA and its association with genes related to hypoxic conditions.
Methods.
We obtained OA-related expression dataset profiles (GSE98918 and GSE55235) from the Gene Expression Omnibus (GEO) repository. By analyzing the differences between these datasets, we identified differentially expressed genes (DEGs) between both OA groups and screened DEGs associated with hypoxia. Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG), and Gene Set Enrichment Analysis (GSEA) were conducted to explore significantly enriched pathways associated with genes related to hypoxia with differential expression in osteoarthritis. We generated protein-protein interaction (PPI) networks and networks of mRNA interactions with miRNA, RNA-binding protein (RBP), and transcription factor (TF). The CIBERSORT algorithm was finally used to explore immune infiltration patterns and examine the effect of inflammation on OA pathogenesis.
Results.
In the GSE98918 dataset, four immune cell types (regulatory T cells (Tregs), M1/M2 Macrophages, and resting Mast cells) demonstrated significant differences (P < 0.05) between OA and Normal groups and exhibited positive correlations. The infiltration levels of these four immune cell were significantly correlated with six hypoxia-related differentially expressed genes. In the GSE55235 dataset, ten immune cell types exhibited significant alterations (P < 0.05) between the OA and Normal groups, with most having negative correlations. These immune cell infiltration abundances were significantly correlated with six hypoxia-related DEGs.
Conclusion.
This study revealed the heterogeneity in immune cell infiltration abundance between OA and normal groups and their relationship with hypoxia-related genes. These discoveries contribute to our understanding of the development of OA and potential therapeutic targets.
High differentiation but low divergence: demographic history of grey reef sharks (Carcharhinus amblyrhynchos) across the Indian Ocean
Authors: Dahms Carolin , Momigliano Paolo 
Published: July 2023

Abstract
Marine species can manifest complex patterns of genetic structure due to factors including large geographic distribution ranges and dispersal barriers, sparse long-distance dispersal and high site fidelity. Understanding these connectivity patterns is vital to determine effective management units prioritising vulnerable or evolutionarily distinct populations. Here we investigate genetic differentiation and divergence among grey reef sharks (Carcharhinus amblyrhynchos), comparing western and central Indian Ocean populations on the one side, and eastern Indian Ocean and western Pacific populations on the other. Recent reports of very high differentiation of central and western Indian Ocean from eastern Indian and Pacific populations warranted investigation into whether central and western Indian Ocean populations represent cryptic species of Evolutionary Significant Units (ESUs). Using reduced representation data from 124 previously sequenced individuals, we confirm high differentiation across the Indo-West Pacific (FST ≈ 0.4). Through demographic reconstruction, we show considerably lower effective population sizes and genetic diversity in the western and central Indian Ocean and strong isolation: migration rates to the central-west Indian Ocean are on the scale of 1–4 migrants per 10 generations, which, nevertheless, may still be sufficient to allow adaptive connectivity, i.e. the spread of adaptive alleles between populations maintaining species cohesiveness. Current levels of divergence, however, are well within the range of observed within-species divergence for marine organisms. Although simulations revealed that net divergence between ocean basins is unlikely to reach the levels of the “grey zone of speciation”, these highly differentiated Indian Ocean populations fulfil several requirements of ESUs, and will benefit from targeted management approaches considering their low genetic diversity and the alarming population declines of Carcharhinids.
The clinical characteristics, gene mutations and outcomes of myelodysplastic syndromes with diabetes mellitus
Authors: Xu Fanhuan , Jin Jiacheng , Guo Juan , Xu Feng , Chen Jianan , Liu Qi , Song Luxi , Zhang Zheng , Zhou Liyu , Su Jiying , Xiao Chao , Zhang Yumei , Yan Meng , He Qi , Wu Dong , Chang Chunkang , Li Xiao , Wu Lingyun 
Published: July 2023

Abstract
Purpose: Diabetes mellitus (DM) is the second most common comorbidity in myelodysplastic syndromes (MDS). The purpose of the study was to investigate the clinical characteristics of MDS patients with DM.
Methods: A retrospective analysis was performed on the clinical data of 958 MDS patients with and without DM. Clinical data, including genetic changes, overall survival (OS), leukemia-free survival (LFS) and infection, were analyzed.
Results: Among 958 patients, 202 (21.1%) had DM. TET2and SF3B1 mutations occurred more frequently in the DM group than in the non-DM group (p = 0.0124 and p = 0.0009, respectively). Compared to non-DM patients, MDS patients with DM had poor OS and LFS (p < 0.0001 and p = 0.0003, respectively), especially in the lower-risk group. In multivariate analysis, DM independently predicted OS (HR 1.369 CI 1.078-1.738, p = 0.010). Meanwhile, DM was an independent risk factor for bacterial infection (HR 2.055 CI 1.415-2.984, p = 0.000) and fungal infection (HR 2.146 CI 1.422-3.238, p = 0.000) in MDS.
Conclusions: MDS patients with DM have an inferior prognosis and higher infection incidence, with TET2 and SF3B1 mutations being more frequent in those cases.
Establishment and characterization of a novel highly aggressive hilar cholangiocarcinoma cell line, CBC3T-1
Authors: Bai Mingzhen , Jiang Ningzu , Fu Wenkang , Huang Chongfei , Tian Liang , Mi Ningning , Gao Long , Ma Haidong , Lu Yawen , Cao Jie , Zhang Chao , Yue Ping , Zhang Yong , Lin Yanyan , Meng Wenbo , Li Xun 
Published: July 2023

Abstract
Background
Cholangiocarcinoma (CCA) is a group of malignant heterogeneous cancers arising from the biliary tree characterized by insidious onset, poor prognosis and high recurrence rate. Immortalized cancer cell lines are the best and easiest models for in vitro and in vivo cancer research.
Methods
CBC3T-1 was originated from a patient with hilar CCA from China and identified by STR and chromosome karyotype analysis. The malignant phenotype was characterized by wound healing assay, transwell migration, Matrigel invasion assay, clone formation assay and in vivo xenograft mouse models. Screening of sensitive clinical anticancer drugs by CCK-8 assay. The cell line was characterized at the genetic level using transcriptome sequencing and whole exome sequencing.
Results
CBC3T-1 was cultured for over 60 passages. Thorough analysis showed that CBC3T-1 cells share characteristics similar to original tumor cells from patients with cholangiocarcinoma and display a stable phenotype, including features of epithelial origin, stem cell-like properties as well as a high invasive and migratory capability and tumorigenicity in mice. Furthermore, this cell line showed the best sensitivity to paclitaxel, followed by gemcitabine. Sequencing results show that cancer-associated pathways and somatic mutations play a dominant role in the development of CCA.
Conclusions
We established and characterized a novel highly aggressive hilar CCA cell line, CBC3T-1, which provides a new model for studying the progression of hilar CCA as well as developing new therapeutic approaches, enriching the heterogeneous in vitro models of CCA.
Comparative Evaluation Of Amount Of Apically Extruded Debris In Endodontic Retreatment Procedures Using Four Different File Systems- An Invitro Study
Authors: Saxena Tarun 
Published: July 2023

Objective(s): To compare the amount of apical debris extruded during endodontic retreatment procedures performed using H files, R-Endo Retreatment files, GPR files and ProTaper Universal Retreatment files.
Materials and Methods: The conventional coronal access cavity was prepared for 40 extracted Mandibular Premolars. The root canals were prepared with ProTaper Next Rotary files till X1. Obturation was done by combination of Lateral and Vertical Compaction technique. All the samples were later attached to Eppendorf tubes. The samples were then divided into four groups each containing 10 samples on the basis of different retreatment files to be used. After complete removal of root canal filling material, the debris and the root filling material remnants adhered was collected in preattached Eppendorf tubes. For final weight computation and comparison, the weight of empty tube was deducted from the weight of tube accommodating the debris. ANOVA and Tukey test were applied for statistical analysis.
Results: The R-Endo retreatment files showed minimum apical extrusion of debris than all the other examined files in the study. The apical extrusion of debris for R Endo min to max was -0.045 to 0.009. However, there was no statistically significant difference in amount of apical extrusion of debris by different files system (p>0.005).
Conclusion(s): There was no significant difference between the groups in apical extrusion of debris. Also rotary retreatment files showed lesser apical of debris than Hedstrom hand files. R Endo extruded less debris apically in this study.
Keywords: Apical extrusion of debris; Gutta-percha; Retreatment files.
Ketogenic Diet Therapy for Glioma: Combined Effects
Authors: Qin Hongyu , Zhang Fan , Gao Shuqing 
Published: July 2023

Abstract:
The ketogenic diet (KD) has made a breakthrough in the treatment of glioma and has become
a research hotspot because of its unique dietary structure, particularly as an auxiliary strategy
for anti-tumor. This article reviews the application and mechanism of KD combined with other antitumor
methods, such as chemoradiotherapy, immunotherapy, molecularly targeted drugs, antiangiogenic
drugs, glucose-targeted drugs, hyperbaric oxygen, ketone bodies, and intestinal flora, aiming
to provide a theoretical basis for the clinical application of KD.
PENGARUH FRAUD DIAMOND TERHADAP KECURANGAN LAPORAN KEUANGAN PADA KABUPATEN DAN KOTA DI PROVINSI JAMBI PERIODE 2014 – 2018
Authors: Fadly Akun , Wahyudi Ilham , Yetti Susfa 
Published: July 2023

Penelitian ini bertujuan untuk menganalisis dan mendapatkan bukti empiris tentang pengaruh fraud diamond (tekanan, kesempatan, rasionalisasi, dan kapabilitas) terhadap kecurangan laporan keuangan di sektor publik. Populasi yang digunakan dalam penelitian ini adalah pemerintah kabupaten dan kota di Provinsi Jambi. Total sampel adalah 55 Pemerintah Kabupaten dan Kota yang diperoleh melalui metode convenience sampling. Penelitian ini menggunakan data sekunder yang diambil dari Laporan Audit Badan Pemeriksa Keuangan 2014-2018. Analisis data yang digunakan dalam penelitian ini adalah analisis regresi berganda dengan bantuan SPSS 22. Hasil penelitian menemukan bahwa secara simultan variabel berpengaruh terhadap kecurangan laporan keuangan dengan tingkat koefisien determinasi sebesar 22,8%. Secara parsial, variabel tekanan tidak berpengaruh terhadap keucran laporan keuagan, variabel kesempatan berpengaruh terhadap kecurangan laporan keuangan, variabel rasionalisasi berpengaruh terhadap kecurangan laporan keuangan dan variabel kapabilitas tidak berpengaruh terhadap kecurangan laporan keuangan.
<strong>Diet-related Lipidomic Signatures and Changed Type 2 Diabetes Risk in A Randomized Controlled Feeding Study with Mediterranean Diet and Traditional Chinese or Transitional diets</strong>
Authors: Luo Yaogan , Sun Liang , Wu Qingqing , Song Boyu , Wu Yanpu , Yang Xiaowei , Zhou Puchen , Niu Zhenhua , Zheng He , Li Huaixing , Gu Weiqiong , Wang Jiqiu , Ning Guang , Zeng Rong , Lin Xu 
Published: July 2023

<p><strong>Objective: </strong>Few trials studied the links of food components in different diets with their induced lipidomic changes and related metabolic outcomes. Thus, we investigated specific lipidomic signatures with habitual diets and modified diabetes risk by using a trial and a cohort.</p>
<p><strong>Research Design and Methods:</strong> We included 231 Chinese with overweight and prediabetes in a randomized feeding trial with Mediterranean diet, traditional or transitional diet (control diet) from February to September 2019. Plasma lipidomic profiles were measured at baseline, third and sixth month by a high-throughput targeted LC-MS. Associations of the identified lipids with habitual dietary intakes were examined in another lipidomic database of Chinese cohort (n=1,117). The relationships between diet-induced changes of lipidomic species and diabetes risk factors were further investigated through both individual lipids and relevant modules in the trial. </p>
<p><strong>Results:</strong> Out of 364 lipidomic species, 26 altered across groups, including 12 triglyceride (TAG) fractions, nine plasmalogens, four phosphatidylcholines (PCs), and one phosphatidylethanolamine. TAG fractions and PCs were associated with habitual fish intake while plasmalogens were associated with red meat intake in the cohort. Of the diet-related lipidomic metabolites, 10 TAG fractions and PC (16:0/22:6) were associated with improved Matsuda index (β = 0.12 to 0.42;<em> PFDR</em> < 0.030). Two plasmalogens were associated with deteriorated fasting glucose (β=0.29 to 0.31; <em>PFDR</em> < 0.014). Similar results were observed for TAG and plasmalogen related modules.</p>
<p><strong>Conclusions:</strong> These fish and red meat related lipidomic signatures sensitively reflected different diets and modified type 2 diabetes risk factors, critical for optimizing dietary patterns.</p>
Studi Pendahuluan: Kepiting Air Tawar (Parathelphusa maindroni) di Kawasan Geopark Merangin Provinsi Jambi
Authors: Wulandari Tia , Ihsan Mahya , Suprayogi Dawam 
Published: July 2023

Geopark Merangin is one of the World’s Natural Heritages (Geological Heritage Site) in Jambi Province which under the auspices of UNESCO. The area has the potential to be developed into an eco-geotourism area. To support this development, monitoring is needed for geological diversity, biodiversity, and cultural diversity. One potential study that can be explored is the existence of freshwater crabs. To date, the existence of freshwater crab species in the waters of Geopark Merangin has not been reported. This study aims to collect freshwater crabs that can be found in the Geopark Merangin area as a preliminary study of freshwater crab diversity in that area. The result indicates that one of the freshwater crab can be found is Parathelphusa maidroni. The presence of P. maidroni in Geopark Merangin area can serve as bioindicator of good water quality and open up potential for the discovery of other species. Futhermore, the discovery of P. maidroni in the aquatic region of Geopark Merangin can be considered as an initial step towards the identification of various freshwater crab species in that area. AbstrakKawasan perairan Geopark Merangin Provinsi Jambi merupakan salah satu warisan alam dunia (Geological Heritage Site) dibawah naungan UNESCO. Kawasan tersebut berpotensi untuk dikembangakan menjadi kawasan Eco-geowisata. Untuk mendukung pengembangan tersebut, perlu dilakukan monitoring baik dari keanekaragaman geologi, keanekaragaman hayati maupun keanekaragaman budaya. Salah satu kajian yang dapat digali potensinya yaitu keberadaan kepiting air tawar. Sampai saat ini belum dilaporkan keberadaan jenis-jenis kepiting air tawar yang ada di perairan Geopark Merangin. Penelitian ini bertujuan untuk mengkaji jenis kepiting air tawar yang dapat ditemukan di kawasan Geopark Merangin sebagai studi pendahuluan terkait keragaman kepiting air tawar yang ada di kawasan tersebut. Hasil penelitian menunjukkan salah satu jenis kepiting air tawar yang dapat ditemukan yaitu Parathelphusa maidroni. Keberadaan P. maidroni di kawasan perairan Geopark Merangin dapat menjadi bioindikator perairan yang masih baik, dan membuka potensi ditemukan jenis kepiting air tawar lainnya. Selain itu dengan ditemukannya P. maidroni di kawasan perairan Geopark Merangin dapat dijadikan sebagai langkah awal untuk dilakukannya pendataan dan eksplorasi jenis-jenis kepiting air tawar di kawasan tersebut.
Biomass and solar energy in buildings, energetic dark greenhouse, an economic approach analysis
Authors: Seraji Mohammad Ali Nasle , Ranjbar Zohreh , Keshavarzzadeh Mansour , Mousavi Shirin , Zahedi Rahim 
Published: July 2023

The use of different energy sources and the worry of running out of some of them in the modern world have made factors such as environmental pollution and even energy sustainability vital. Vital resources for humanity include water, environment, food, and energy. As a result, building strong trust in these resources is crucial because of their interconnected nature. Sustainability in security of energy, water and food, generally decreases costs and improves durability. This study introduces and describes the components of a system named “Desktop Energetic Dark Greenhouse” in the context of the quadruple nexus of water, environment, food, and energy in urban life. This solution can concurrently serve to strengthen the sustainable security of water, environment, food, and energy. For home productivity, a small-scale version of this project was completed. The costs and revenues for this system have been determined after conducting an economic study from the viewpoints of the investor and the average household. The findings indicate that the capital return period is around five years from the investor’s perspective. The capital return on investment for this system is less than 4 years from the standpoint of the households. According to the estimates, this system annually supplies about 20 kg of vegetables or herbs, which means about one third of the annual needs of a family.
Geodynamic Studies in the Pieniny Klippen Belt in 2004–2020
Authors: Staniszewska Dominika , Liwosz Tomasz , Pachuta Andrzej , Próchniewicz Dominik , Szpunar Ryszard 
Published: July 2023

ABSTRACT
The Pieniny Geodynamic Test Field is situated in the middle of the region between the Inner and Outer Carpathians. Geodynamic research conducted in the past in the Pieniny Klippen Belt (PKB) region were suggestive of neotectonic activity. The goal of the investigation was to determine whether the nearby structures, the Podhale Flysh (FP) and the Magura Nappe (MN), are affected by neotectonic activity in the PKB. The goal of the study was to ascertain the velocity and direction of motion of stations situated close to the Pieniny Geodynamic Test Field’s 3 main structures. Twelve GNSS stations, including 6 in the PKB, 3 in the MN, and 3 in the FP, make up the Pieniny Geodynamic Test Field. Three GNSS sites in the Tatra Mountains (TM) complete the entire geodynamic test field. The satellite observations made between 2004 and 2020 (excluding the year 2005 due to lack of observation) were investigated to identify the horizontal movements. Using the IGb14 reference system, the station’s positions and velocities were calculated. First, daily sessions were used to process the horizontal coordinates of the points for an average observation epoch in a given year. Sixteen measurement epochs were included in the long-time solution. Based on the horizontal velocity residues in the north–south and east–west directions, the station’s movement was calculated. The collected results were compared to information from the EUREF Permanent GNSS Network (EUREF) and to the findings of prior research on the tectonic activity of the PKB. The results of horizontal displacements calculated using GNSS measurements in the area of the PKB and nearby structures—the MN and the FP are presented and analyzed in this article.