bluuebunny/crossref_metadata_2025_split huggingface dataset:
Pan-cancer analysis revealing that PTPN2 is a indicator of risk stratification for acute myeloid leukemia identification of high-risk patients
Authors: wang xuanyu , Wu Sanyun , Sun Le , Jin Peipei , Zhang Jianmin , Liu Wen , Zhan Zhuo , Wang Zisong , Liu Xiaoping , He Li 
Published: June 2023

Abstract
Background The non-receptor protein tyrosine phosphatases gene family (PTPNs) is involved in the tumorigenesis and development of many cancers, but the role of PTPNs in acute myeloid leukemia (AML) remains unclear.Methods After a comprehensive evaluation on the expression patterns and immunological effects of PTPNs using a pan-cancer analysis based on RNA sequencing data obtained from The Cancer Genome Atlas (TCGA), the most valuable gene PTPN2 was discovered. Further investigation of the expression patterns of PTPN2 in different tissues and cells showed a robust correlation with AML. PTPN2 was then systematically correlated with immunological signatures in the AML tumor microenvironment (TME) and its differential expression was verified using clinical samples. In addition, a prediction model, being validated and compared with other models, was developed in our research.Results The systematic analysis of PTPN family reveals that the effect of PTPNs on cancer may be correlated to mediating cell cycle-related pathways. It was then found that PTPN2 was highly expressed in hematologic diseases and bone marrow tissues, and its differential expression in AML patients and normal humans was verified by clinical samples. Based on its correlation with immune infiltrates, immunomodulators, and immune checkpoint (ICP), PTPN2 was found to be a reliable biomarker in the immunotherapy cohort and a prognostic predictor of AML. And PTPN2'riskscore (PTPRS) can accurately predict the prognosis and response of cancer immunotherapy.Conclusions These findings revealed the correlation between PTPNs and immunophenotype, which may be related to cell cycle. PTPN2 was differentially expressed between clinical AML patients and normal people. It is a diagnostic biomarker and potentially therapeutic target, providing targeted guidance for clinical treatment.
Precise localization and protective strategies of MMA during cerebral revascularization ----a set of modified craniotomy procedures combined with simple virtual reality technology based on 3D Slicer
Authors: Liu Chuanghong , Xu Hong , Kong Gang 
Published: June 2023

Abstract
The frontal branch of middle meningeal artery (MMA) can easily be damaged during revascularization surgery. To precise locate it and minimize its injury, we propose a set of modified craniotomy procedures combined with simple virtual reality (VR) technology based on three-dimensional (3D) Slicer simply, economically, and efficiently. Patients with Moyamoya disease (MMD) and internal cerebral artery occlusion (ICAO) who received revascularization from January 2015 to December 2022 were divided into two groups based on the methods used to locate the MMA: traditional methods and precise MMA locating with VR technology. Patient demographics and clinical characteristics were analyzed to compare the preservation rates of MMA. The distances between this artery and bony anatomical landmarks were also measured to better understand its localization. There was no significant difference in baseline characteristics between the two groups. The precise MMA locating group exhibited a significantly higher preservation rate of the frontal branch of MMA (p = 0.037, 91.7% vs. 68.2%). Over 77% of patients had their frontal branch of MMA partially or completely surrounded by bony structures to varying degrees. Therefore, the combination of modified craniotomy procedures, 3D Slicer, and simple VR technology represents an economical, efficient, and operationally simple strategy.
Quorum-sensing synthase mutations re-calibrate autoinducer concentrations in clinical isolates of Pseudomonas aeruginosa to enhance pathogenesis
Authors: Paczkowski Jon , Simanek Kayla , Schumacher Megan , Mallery Caleb , Shen Stella , Li Lingyun 
Published: June 2023

Abstract
Quorum sensing is a mechanism of bacterial communication that controls virulence gene expression. Pseudomonas aeruginosa regulates virulence via two synthase/transcription factor receptor pairs: LasI/R and RhlI/R. LasR is considered the master transcriptional regulator of quorum sensing, as it upregulates rhlI/R. However, clinical isolates often have frequent inactivating mutations in lasR, while maintaining Rhl-dependent signaling. We sought to understand how quorum sensing progresses in isolates with lasR mutations, specifically via activation of RhlR. We found that clinical isolates with lasR inactivating mutations often harbored concurrent mutations in rhlI. Using ultra-high-performance liquid chromatography coupled with high-resolution mass spectrometry, we discovered that strains lacking lasR overproduced the RhlI-synthesized autoinducer and that RhlI variants re-calibrated autoinducer concentrations to wild-type levels, restoring virulent phenotypes. These findings provide the molecular basis for the plasticity of quorum sensing in an infection niche.
A Countertraction Closed Reduction Technique in Minimally Invasive Fixation of Recent Type C Pelvic Ring Injuries
Authors: Liu Wei , Zhao Jinmin , Cheng Jianwen , Huang Linke , Shi Xiaorong , Ning Chao , Jiang Jie , Hu Feng 
Published: June 2023

Abstract
Purpose: This study demonstrated a novel traction technique for closed reduction called countertraction closed reduction technique (CCRT) and evaluated its effectiveness.
Methods: The data of Patients with unstable pelvic fractures treated with CCRT and minimally invasive fixation for unstable pelvic fracture, admitted to the second affiliated hospital of Guangxi Medical University from January 2017 to February 2022, were analyzed retrospectively. The CCRT was performed in patients with a fresh Tile C pelvic fracture. A sacroiliac screw was then placed to fix the posterior pelvic ring, and internal or external fixation was used to fix the anterior pelvic ring. Operation time, intraoperative blood loss, and duration of hospital stay were recorded, and fracture union and postoperative complications were evaluated. Fracture reduction quality was evaluated using the Matta score. Functional recovery and general quality of life were evaluated using the Majeed score.
Results: Thirteen patients (nine males and four females), with an average age of 49.6 years were followed up for a mean of 18.5 months. The average operation time was 137.2 minutes (range 92–195 minutes), the average intraoperative blood loss was 31.2 ml (range 10–120 ml) and the average duration of hospital stay was 14.3 days (range 4-32 days). All patients achieved bony union with an average union time of 11.9 weeks (range 10-16 weeks). According to the Matta radiographic criteria, the quality of fracture reduction was excellent in eight patients, good in four, and fair in one. The average Majeed functional score was 89.7 (range 78-100). The functional evaluation revealed that the outcomes were excellent in nine patients, and good in four patients. Complications included incision fat liquefaction in one patient, and heterotopic ossification in another patient. There were no surgical complications as a result of CCRT.
Conclusion: CCRT is an alternative closed reduction method for minimally invasive fixation of fresh Tile C1 and C2 pelvic fractures. Its advantages include a small surgical incision, decreased intraoperative bleeding, satisfactory fracture reduction, bone healing and functional recovery.
DRD4 Gene polymorphism and impulse control disorder induced by dopamine agonists in Parkinson's disease.
Authors: Valldeoriola Francesc , Ballesteros Viviana Torres , Peréz-Montesino Jesica , Fernández-Santiago Ruben , Fernandez Manel , Cámara Ana , Compta Yaroslau , Marti M. Josep , Beltran Àlex Guerra , Rios José , Ezquerra Mario 
Published: June 2023

Abstract
Impulse control disorders and their consequences display variability among individuals, indicating potential involvement of environmental and genetic factors. We analyzed a cohort of Parkinson's disease patients treated with dopamine agonists and investigated the influence of the dopamine D4 receptor gene polymorphism, DRD4 7R+, which is linked to psychiatric disorders, impulsive traits, and addictive behaviors. We found that DRD4 7R + is a significant genetic risk factor associated with the severity of ICD.
Postmolar choriocarcinoma with solitary renal metastasis in the absence of primary uterine tumor: A case report and review of the literature
Authors: Geravandi Mahsa , Hajihashemi Ali , Adibi Atoosa , Tirtashi Reza Habibi 
Published: June 2023

Abstract
Background: Choriocarcinoma is a rare high malignant type of gestational trophoblastic disease (GTD) that occurs after pregnancies, abortions, or hydatiform moles and seldom metastasizes to the kidneys. Postmolar choriocarcinoma with renal metastasis is also scarce and was not frequently described. Here, we depicted the first case of postmolar choriocarcinoma with solitary kidney metastasis presenting with spontaneous renal hemorrhage (SRH) in the absence of a primary uterine tumor.
Case presentation: A 41-year-old female patient with a history of complete molar pregnancy was evaluated due to severe flank pain, nausea/vomiting, gross hematuria, and vaginal bleeding in the last three weeks. The evaluations demonstrated bilateral pleural effusion without any lesion within the lungs, an empty uterine cavity, and a left kidney lower pole lesion with active bleeding surrounded by hematoma, alongside a serum β-hCG level of 60,000 mIU/mL. According to these findings, laparotomy exploration was planned and ended with a partial nephrectomy, para ovarian cystectomy. In addition, endometrial curettage was performed. The histopathology results revealed choriocarcinoma renal metastasis with high expression of β-hCG, cytokeratin, cytokeratin7, and KI67. In addition, a corpus luteum cyst was found within the left ovarian cyst, and endometrial curettage showed a progestin agent effect without malignant cells. Moreover, the brain, liver, and adrenal glands were not involved with metastases, and the pleural effusion fluid was free of malignant cells. Finally, the patient was referred to the oncology department to receive chemotherapy, and the β-hCG level dropped to 5 mIU/mL after receiving courses of a standard EMA-CO regimen (etoposide, methotrexate, actinomycin D, cyclophosphamide, and vincristine/oncovin) over three weeks.
Conclusions: Postmolar metastatic choriocarcinoma might present only urologic symptoms, such as hematuria and SRH, due to metastatic renal involvement. Indeed, considering choriocarcinoma and evaluating the serum β-hCG level among childbearing-aged females with unexplained urological symptoms could be advantageous, particularly when a previous molar pregnancy exists.
Use of platelet count on admission for determining illness severity and predicting prognosis in acute cholangitis patients
Authors: Chen Xiaoying , Hou Xueping , Zhang Dan , Liu Jinglun , Tian Shijing 
Published: June 2023

Abstract
Background
Thrombocytopenia often occurs in patients with acute cholangitis and is a risk factor for poor prognosis in acute cholangitis. The study was to investigate the relationship between platelet count and illness severity and their performances in predicting hospital mortality.
Methods
This was a single-center, retrospective, observational cohort study. We analyzed 498 patients with acute cholangitis who were admitted to our hospital between July 2013 and July 2021. The correlation between admission platelet count and severity and prognosis of patients was analyzed.
Results
1) In this study, the incidence of acute cholangitis thrombocytopenia (platelet count < 100×109/L) and severe thrombocytopenia (platelet count < 50×109/L) were 31.12% and 7.43%, respectively. 2) Platelet count of the Thrombocytopenia group was 64(42, 79) × 109/L. Compared with the NO-Thrombocytopenia group, the Thrombocytopenia group had more Grade III patients, higher complication rates (respiratory failure, circulatory failure, bacteremia), and higher APACHE II score and SOFA score (P < 0.05). 3) The 60-day mortality rate was significantly higher in the Thrombocytopenia group than that in the NO-Thrombocytopenia group (12.90% vs. 2.33%, P < 0.001). Binary logistic regression analysis demonstrated that platelet count at hospital admission was a risk factor for death. 4) ROC curve analysis showed that platelet count cut-off value for predicting the death of patients with acute cholangitis was 84×109/L, with sensitivity of 0.764, specificity of 0.714, and an AUC of 0.760.
Conclusions
Platelet count is a readily available, prognostic marker that can identify acute cholangitis patients with poorer outcome.
Clinical and Immunological Outcomes of HIV-Exposed Uninfected and HIV-Unexposed Uninfected Children in the First 24 Months of Life in Western Kenya
Authors: Ray Jessica E. , Dobbs Katherine R. , Ogolla Sidney O. , Daud Ibrahim I. , Midem David , Omenda Maxwel M. , Nowacki Amy S. , Beeson James G. , Sabourin Katherine R. , Rochford Rosemary , Dent Arlene E. 
Published: June 2023

Abstract
Background
Previous studies show increased morbidity in children who are HIV-exposed but uninfected (HEU) compared to children who are HIV-unexposed uninfected (HUU). We sought to evaluate the effects of prenatal HIV exposure on clinical and immunological outcomes in the first 24 months of life.
Methods
Eighty-five HEU and 168 HUU children from Kenya were followed from birth to 24 months. All mothers with HIV received combination antiretroviral therapy. HEU children received standard-of-care cotrimoxazole prophylaxis through 18 months. Episodes of acute illness were identified through a combination of active and passive follow up. Trajectories of plasma cytokines, vaccine-specific antibodies, and antimalarial antibodies were examined.
Results
HEU and HUU children had similar growth curves. HEU children had lower rates of malaria and respiratory illness. Trajectories of plasma cytokines and vaccine-specific antibodies were similar in HEU and HUU children. There were subtle differences in antimalarial antibody dynamics, in which HEU children had overall lower antibody levels against five of the 14 malaria antigens tested.
Conclusions
HEU children born to optimally treated mothers living with HIV had similar growth characteristics and immune profiles compared to HUU children. HEU children had reduced risk for malaria and respiratory illness, which may be secondary to cotrimoxazole prophylaxis.
Investigation of mechanical and thermal behavior of fiber-reinforced silica aerogel composites
Authors: zubair Muhammad , Ammar Muhammad , Wakeel Aneela , Nasir Muhammad Ali 
Published: June 2023

Abstract
Fiber-reinforced silica xerogels are extensively utilized in various applications due to their versatility. This research focuses on examining the influence of silica gel fiber reinforcement on the physical properties of silica xerogel. The investigation encompasses an analysis of the morphology, mechanics, and thermal behavior. Through scanning electron microscopy (SEM), it has been observed that glass fiber exhibits lower adhesion to the xerogel network compared to carbon fiber. The hydrophobicity of the composite silica xerogel, confirmed by Fourier Transform Infrared (FTIR) analysis, is attributed to the absence of the OH group. When subjected to a strain rate of 48%, the carbon fiber composite demonstrates superior compressive stress endurance compared to the glass fiber composite. Furthermore, the carbon fiber composite experiences a 1% lower weight loss than the glass fiber composite, which enhances its suitability for reinforcing the characteristics of silica xerogel. Consequently, the composite silica aerogel, combining carbon fiber and silica gel, exhibits exceptional compressive strength, low density, and hydrophobic properties.
Teacher-Student Relationship and Prospect Identity among Students: The Mediating Role of Social-Emotional Competence and Student Satisfaction
Authors: Du Yue , Qi Zhanyong 
Published: June 2023

Abstract
Background. Prospect identity of the major of secondary vocational students plays a vital role in the student's future growth and work. This study aimed to investigate the link between factors in the teacher-student relationship and prospect identity of students' majors in secondary vocational schools. We studied social-emotional competence and student satisfaction as mediating factors, and created a chain of mediators.
Sample.We collected data from 6,497 secondary vocational school students in a city in western China.
Methods.Bootstrapping analyses were employed to test the direct and the mediating effects between the teacher-student relationship and students’ prospect Identity. The students reported on four variables: teacher-student relationships, social-emotional competence, satisfaction, and prospect identity.
Results.The measurement results showed the teacher-student relationship affects prospect identity directly or indirectly through social-emotional competence and student satisfaction, and there is a chain mediating role of social-emotional competence and student satisfaction.
Conclusions. We conclude that it remains essential to improve the quality of interaction-based teacher-student relationship to enhance student continuity and stability in a major development.
Development of novel prognostic score based on the indicators reflecting the tumor glandular differentiation and microenvironment for patients with colorectal cancer
Authors: Kobayashi Toshinori , Ishida Mitsuaki , Miki Hisanori , Yagyu Takuki , Hatta Masahiko , Hamada Madoka , Hirose Yoshinobu , Sekimoto Mitsugu 
Published: June 2023

Abstract
Purpose: Development of prognostic stratification in patients with colorectal cancer (CRC) is an urgent concern. Desmoplastic reaction (DR) is a tissue reaction, speculated to represent the tumor microenvironment. DR types have been established as independent prognostic indicators in CRC. However, DR has not been included in the prognostic nomograms. We aimed to analyse the prognostic significance of combined histopathological indicators reflecting both tumor glandular differentiation and microenvironment.
Methods: We selected 331 consecutive patients with CRC who underwent surgical resection in the Department of Surgery at Kansai Medical University (KMU). Histological glandular differentiation grading scores (HGDSs) were classified as 2 (0 point), 3-4 (1 point), and 5-6 (2 points). Tumor buddings (TBs) were classified as TB1 (0 point), TB2 (1 point), and TB3 (2 points). Lymph node metastasis was classified as pN0 (0 point), pN1 (1 point), and pN2 (2 points). pT1 or 2 was considered as 0 point; pT3 or 4 + DR non-immature type, 1 point; and pT3 or 4 + DR immature type, 2 points. Preoperative carcinoembryonic antigen (CEA) levels were classified as <5.0 ng/mL (0 point) and ≧5.0 (1 point). After summing these five factors (0-9 points), scores I (0-2 points), II (3-4 points), III (5-6 points), and IV (7-9 points) were defined.
Results: The Kaplan-Meier curves showed significant differences in disease-specific survivals among scores, which were more useful than those in the current AJCC Eighth Classification, especially in patients with p-stages I, II, and III.
Conclusion: The usefulness of this scoring system was demonstrated. This is the first prognostic nomogram to include DR.
Aspergillus spp. associated with Aspergillus vine canker in Mexico
Authors: Hernandez-Martinez Rufina 
Published: June 2023

Abstract
The genus Aspergillus encompasses species with high economic and social impact. Species belonging to section Nigri known as black aspergilli, have predominantly been isolated from soil and plants. Fungi associated with Aspergillus vine canker infect vigorous new shoots and canes during plant training, entering through wounds. Recently, grapevines exhibiting symptoms resembling Aspergillus vine canker have been observed in Mexico. Thus, this study aimed to investigate these diseased plants. Woody samples from symptomatic plants were surface sterilized with alcohol and fire and then placed onto Potato Dextrose Agar (PDA) medium. Seventeen isolates displaying morphology similar to the genus Aspergillus were obtained. Colony and microscopic characteristics were observed on Czapek Yeast Extract Agar (CYA) and Malt Extract Agar (MEA). Phylogenetic analysis using calmodulin (CaM) and β-tubulin (BenA) gene markers, revealed the presence of Aspergillus niger, A. tubingensis, and A. welwitschiae species. In pathogenicity assays, A. niger A10BCMX, A. niger A8SMX, and A. tubingensis A13SMX exhibited the highest virulence, resulting in lesions up to 2 cm in length after 50 days of inoculation. Powdery black conidia were found within the wound, specifically in the cambium. In addition to necrotic lesions, mycelia and black conidia were observed on the leaves. This study represents the first report of Aspergillus species associated with Aspergillus vine canker in Mexican vineyards and the first report worldwide of A. welwitschiae associated with this disease.
Humidity may amplify the temperature-related health risks in the context of climate change
Authors: Huang Cunrui , He Yiling , Dong Wei , Zhao Liang , Wang Huibing , Cai Wenjia , Liu Zhao , Lin Hualiang , Ma Wenjun 
Published: June 2023

AbstractHumidity and temperature may pose joint effects on human health. However, current temperature-related risk assessments generally focus on univariate temperature statistics, leading to inaccurate risk estimation. By combining temperature and humidity intensities as compound temperature-humidity events (hereinafter as compound events), we examined the effects of humidity in temperature-related health risks in the context of climate change in China using ambulance dispatch data, and further determined the high-risk types and thresholds of compound events. Results reveal that humidity can amplify the temperature-related health risks. Higher risks were found during cold and dry events and warm and wet events. Compound events mainly occurred in Southeast China during 1979–2019, while the frequency and gripped regions are projected to increase by 2100, and climate change will amplify these trends. Since humidity would exacerbate temperature-related health risks, therefore multiple meteorological parameters are needed in defining adverse weather conditions and conducting risk assessment accurately.