bluuebunny/crossref_metadata_2025_split huggingface dataset:
Deciphering the sex bias in housekeeping gene expression in adipose tissue: a comprehensive meta-analysis of transcriptomic studies
Authors: Guaita-Cespedes Maria , Grillo-Risco Rubén , Hidalgo Marta R. , Fernández-Veledo Sonia , Burks Deborah Jane , de la Iglesia-Vayá María , Galán Amparo , Garcia-Garcia Francisco 
Published: April 2023

Abstract
Background
As the housekeeping genes (HKG) generally involved in maintaining essential cell functions are typically assumed to exhibit constant expression levels across cell types, they are commonly employed as internal controls in gene expression studies. Nevertheless, HKG may vary gene expression profile according to different variables introducing systematic errors into experimental results. Sex bias can indeed affect expression display, however, up to date, sex has not been typically considered as a biological variable.

Methods
In this study, we evaluate the expression profiles of six classical housekeeping genes (four metabolic: GAPDH, HPRT, PPIA, and UBC, and two ribosomal: 18S and RPL19) to determine expression stability in adipose tissues (AT) of Homo sapiens and Mus musculus and check sex bias and their overall suitability as internal controls. We also assess the expression stability of all genes included in distinct whole-transcriptome microarrays available from the Gene Expression Omnibus database to identify sex-unbiased housekeeping genes (suHKG) suitable for use as internal controls. We perform a novel computational strategy based on meta-analysis techniques to identify any sexual dimorphisms in mRNA expression stability in AT and to properly validate potential candidates.

Results
Just above half of the considered studies informed properly about the sex of the human samples, however, not enough female mouse samples were found to be included in this analysis. We found differences in the HKG expression stability in humans between female and male samples, with females presenting greater instability. We propose a suHKG signature including experimentally validated classical HKG like PPIA and RPL19 and novel potential markers for human AT and discarding others like the extensively used 18S gene due to a sex-based variability display in adipose tissue. Orthologs have also been assayed and proposed for mouse WAT suHKG signature. All results generated during this study are readily available by accessing an open web resource (https://bioinfo.cipf.es/metafun-HKG) for consultation and reuse in further studies.

Conclusions
This sex-based research proves that certain classical housekeeping genes fail to function adequately as controls when analyzing human adipose tissue considering sex as a variable. We confirm RPL19 and PPIA suitability as sex-unbiased human and mouse housekeeping genes derived from sex-specific expression profiles, and propose new ones such as RPS8 and UBB.
Constructing a knowledge graph for open government data: the case of Nova Scotia disease datasets
Authors: Rajabi Enayat , Midha Rishi , de Souza Jairo Francisco 
Published: April 2023

AbstractThe majority of available datasets in open government data are statistical. They are widely published by various governments to be used by the public and data consumers. However, most open government data portals do not provide the five-star Linked Data standard datasets. The published datasets are isolated from one another while conceptually connected. This paper constructs a knowledge graph for the disease-related datasets of a Canadian government data portal, Nova Scotia Open Data. We leveraged the Semantic Web technologies to transform the disease-related datasets into Resource Description Framework (RDF) and enriched them with semantic rules. An RDF data model using the RDF Cube vocabulary was designed in this work to develop a graph that adheres to best practices and standards, allowing for expansion, modification and flexible re-use. The study also discusses the lessons learned during the cross-dimensional knowledge graph construction and integration of open statistical datasets from multiple sources.
FACI is a novel clathrin adaptor protein 2-binding protein that facilitates low-density lipoprotein endocytosis
Authors: Cheng Yun , Kang Xiao-Zhuo , Chan Pearl , Cheung Pak-Hin Hinson , Cheng Tao , Ye Zi-wei , Chan Chi-Ping , Yu Cheng-Han , Jin Dong-Yan 
Published: April 2023

Abstract
Background
Cholesterol plays a vital role in multiple physiological processes. Cellular uptake of cholesterol is mediated primarily through endocytosis of low-density lipoprotein (LDL) receptor. New modifiers of this process remain to be characterized. Particularly, the role of fasting- and CREB-H-induced (FACI) protein in cholesterol homeostasis merits further investigation.

Methods
Interactome profiling by proximity labeling and affinity purification − mass spectrometry was performed. Total internal reflection fluorescence microscopy and confocal immunofluorescence microscopy were used to analyze protein co-localization and interaction. Mutational analysis was carried out to define the domain and residues required for FACI localization and function. Endocytosis was traced by fluorescent cargos. LDL uptake in cultured cells and diet-induced hypercholesterolemia in mice were assessed.

Results
FACI interacted with proteins critically involved in clathrin-mediated endocytosis, vesicle trafficking, and membrane cytoskeleton. FACI localized to clathrin-coated pits (CCP) on plasma membranes. FACI contains a conserved DxxxLI motif, which mediates its binding with the adaptor protein 2 (AP2) complex. Disruption of this motif of FACI abolished its CCP localization but didn’t affect its association with plasma membrane. Cholesterol was found to facilitate FACI transport from plasma membrane to endocytic recycling compartment in a clathrin- and cytoskeleton-dependent manner. LDL endocytosis was enhanced in FACI-overexpressed AML12 cells but impaired in FACI-depleted HeLa cells. In vivo study indicated that hepatic FACI overexpression alleviated diet-induced hypercholesterolemia in mice.

Conclusions
FACI facilitates LDL endocytosis through its interaction with the AP2 complex.
ICU admission for solid cancer patients treated with immune checkpoint inhibitors
Authors: Toffart Anne-Claire , Meert Anne-Pascale , Wallet Florent , Gibelin Aude , Guisset Olivier , Gonzalez Frédéric , Seguin Amélie , Kouatchet Achille , Delaunay Myriam , Debieuvre Didier , Duchemann Boris , Rousseau-Bussac Gaëlle , Nyunga Martine , Grimaldi David , Levrat Albrice , Azoulay Elie , Lemiale Virginie 
Published: April 2023

Abstract
Background
Immune checkpoint inhibitors (ICI) have revolutionized the management of cancer. They can induce immune-related adverse events (irAE) leading to intensive care unit (ICU) admission. We aimed to describe irAEs for ICU admissions in solid cancer patients treated with ICIs.

Methods
This prospective multicenter study was conducted in France and Belgium. Adult patients with solid tumor and treated with systemic ICIs within the last 6 months, requiring non-programmed ICU admission were included. Patients admitted for microbiologically documented sepsis were excluded. Imputability of irAEs in ICU admissions was described according to the WHO-UMC classification system at ICU admission and at ICU discharge. The use of immunosuppressant treatment was reported.

Results
115 patients were eligible. Solid tumor was mainly lung cancer (n = 76, 66%) and melanoma (n = 18, 16%). They were mainly treated with an anti-PD-(L)1 alone (n = 110, 96%). Main ICU admission reasons were acute respiratory failure (n = 66, 57%), colitis (n = 14, 13%), and cardiovascular disease (n = 13, 11%). ICU admission was considered “likely” associated with irAE for 48% (n = 55) of patients. Factors independently associated with irAE were a good ECOG performance status (PS) (ECOG-PS of 0 or 1 vs. ECOG-PS of 2–3, odds ratio [OR] = 6.34, 95% confidence interval [95% CI] 2.13–18.90, and OR = 3.66, 95% CI 1.33–10.03, respectively), and a history of irAE (OR = 3.28, 95% CI 1.19–9.01). Steroids were prescribed for 41/55 (75%) patients with ICU admission “likely” related to irAE. Three patients were subsequently treated with immunosuppressants.

Conclusion
IrAEs accounted for half of ICU admissions in cancer patients receiving ICIs. They could be treated with steroids. Identifying the imputability of irAEs in ICU admissions remains a challenge.
On the Durrmeyer variant of q-Bernstein operators based on the shape parameter λ
Authors: Su Lian-Ta , Aslan Reşat , Zheng Feng-Song , Mursaleen M. 
Published: April 2023

AbstractIn this work, we consider several approximation properties of a Durrmeyer variant ofq-Bernstein operators based on Bézier basis with the shape parameter$\lambda \in[ -1,1]$λ∈[−1,1]. First, we calculate some moment estimates and show the uniform convergence of the proposed operators. Next, we investigate the degree of approximation with regard to the usual modulus of continuity, for elements of Lipschitz-type class and Peetre’sK-functional, respectively. Finally, to compare the convergence behavior and consistency of the related operators, we demonstrate some convergence and error graphs for certain$\lambda \in[ -1,1]$λ∈[−1,1]andq-integers.
Asset management analytics for urban water mains: a literature review
Authors: Delnaz Atefeh , Nasiri Fuzhan , Li S. Samuel 
Published: April 2023

AbstractThis study presents a review of the state-of-the-art literature on water pipe failure predictions, assessment of water losses risk, optimal pipe maintenance plans, and maintenance coordination strategies. In addition, it provides a categorization of water main (WM) failures as well as a taxonomy of WM maintenance strategies. In particular, predictive and prescriptive analytics are highlighted with the investigation of their contributions and drawbacks from methodological and application perspectives. This review aims at providing a review of failure analytics developed recently in water mains domain either for prediction of failure or identification of optimal maintenance strategies conjointly. Future research directions and challenges are elaborated in advancing the understanding about the mechanisms leading to failures. The existing gaps between theory and practice in managing assets across water distribution networks ensuring cost-effectiveness and reliability are discussed. As knowledge about the state of the water mains and related areas is crucial, thus, this review provides an state-of-the-art update from recent studies, and accordingly, presents and discusses avenues for future research.
“Major pathologic response” in lymph nodes: a modified nodal classification for non-small cell lung cancer patients treated with neoadjuvant immunochemotherapy
Authors: Deng Hongsheng , Xiong Shan , Zhong Ran , Zheng Yongmei , Liang Hengrui , Cheng Bo , Li Jianfu , Li Feng , Chen Zhuxing , Wang Haixuan , He Jianxing , Liang Wenhua 
Published: April 2023

AbstractWe aim to examine the prognostic value of major pathologic response in metastatic lymph nodes (mLN-MPR) after immunochemotherapy in non-small cell lung cancer (NSCLC), and demonstrate the pathological characteristic of regression in mLN. Adult patients consecutively undergone neoadjuvant immunochemotherapy and radical-intent surgery for initial stage cIII NSCLC between 2020 and 2021 were included. Hematoxylin- and eosin-stained slides of paraffinembedded sections of the degree of pathologic response in the primary tumor (PT) and its paired involved LNs were reviewed. Imaging mass cytometry was conducted to quantify the immunological status. With 10% as residual viable tumor (RVT) cutoff, mLN-MPR (HR: 0.34, 95%CI: 0.14–0.78; P = 0.011, ref: mLN-MPR(-)) showed more significant correlation with DFS than ypN0 (HR: 0.40, 95%CI: 0.17–0.94; P = 0.036, ref: ypN1-N2). And mLN-MPR combined with PT-MPR, compared with ypN stage combined with PT-MPR (p-value: 0.030 vs. 0.117), can better distinguished the DFS curves of the 4 subgroups of patients. mLN-MPR(+)/PT-MPR(+) patients had the best prognosis compared with other subgroups. Pathologic responses of RVT in PT and paired regional LNs [MPR inconsistency rate: 21/53 (39.6%)], and across different LNs could be inconsistent, especially in squamous cell carcinoma. RVT% in mLNs after immunochemotherapy appeared to be polarized [16 (30.2%) cases with RVT ≥ 70%; 34 (64.2%) with RVT ≤ 10%]. Partial regression of LN metastasis could present with distinct immune subtypes: immune-inflamed or immune-evacuation subtype, and the former presented with higher CD3, CD8, and PD-1 expression in the invasive margin. mLN-MPR demonstrated a potential prognostic value in predicting DFS in patients treated with neoadjuvant immunochemotherapy, but further research is needed to validate its usefulness for other survival outcomes, including OS.
Subjective social status and well-being of adolescents and young adults in Ghanaian schools: conditional process analysis
Authors: Quansah Frank , Agormedah Edmond Kwesi , Hagan John Elvis , Frimpong James Boadu , Ankomah Francis , Srem-Sai Medina , Dadaczynski Kevin , Okan Orkan , Schack Thomas 
Published: April 2023

AbstractBackgroundWith the growing concern and interest in the mental health and well-being of adolescents and young adults (AYAs) including those in schools, many studies have explored the bivariate relationship between subjective social status (SSS) and AYAs’ subjective well-being (SWB). Acknowledging the spurious nature of this relationship, we assessed the relationship between SSS and SWB of AYAs in schools within Northern Ghana, focusing on the conditional indirect effect of monetary resource (MR) and sense of coherence (SoC).MethodsWe utilised a cross-sectional descriptive design to survey 1096 senior high school students from two regions in Ghana’s Northern zone through a stratified sampling method. A questionnaire consisting of a number of calibrated standardized measures was used for the data collection. The data were processed using SPSS and PROCESS Macro and analysed using Hayes’ conditional process analysis.ResultsThe results revealed that students’ MR significantly moderated the relationships between SSS and SoC as well as SSS and SWB. A significant moderated mediation effect of MR and SoC on the relationship between SSS and SWB was found. Particularly, AYAs who reported higher levels of MRl, SSS and SoC reported a better SWB.ConclusionThe findings underscore the relevance of providing sufficient financial support for students in secondary schools in Ghana; thus, highlighting the sheer relevance of economic capital as a leading factor for better well-being. The findings also place much emphasis on building students’ personal coping mechanisms as a key variable in explaining how the students’ SSS and MR translate into having positive mental health outcomes.
Suicidality and homelessness: prevalence and associated factors of suicidal behaviour among homeless young adults in Southern Ethiopia
Authors: Yohannes Kalkidan , Gezahegn Melkamu , Birhanie Mekonnen , Simachew Yilkal , Moges Awoke , Ayano Getinet , Toitole Kusse Koirita , Mokona Hirbaye , Abebe Lulu 
Published: April 2023

Abstract
Background
Research indicates that homelessness is associated with an increased risk of suicide. While street homelessness is a global problem, it is a disproportionately serious concern in low- and middle-income countries such as Ethiopia. Despite their high risk of suicidal thoughts and attempts, there has been limited research on this subject among homeless young people in Ethiopia. Therefore, we assessed the prevalence and factors contributing to suicidal behaviour among homeless young people in the southern region of this country.

Methods
We conducted a community-based cross-sectional study from 15 June to 15 August 2020 involving 798 homeless young adults in four southern Ethiopian towns and cities. The Suicide Behaviour Questionnaire-Revised (SBQ-R) was used to assess suicidal behaviour. Data were coded and entered into Epi-Data version 7 and analysed using SPSS version 20. We conducted a multivariable logistic regression analysis to identify factors associated with suicidal behaviour. Variables with a p-value of < 0.05 were considered statistically significant. An adjusted odds ratio with a 95% confidence interval was determined to indicate the association’s strength.

Results
The overall prevalence of suicidal behaviour among young homeless individuals was 38.2% (95% CI: 34.8%, 41.5%). The lifetime prevalence of suicidal ideation, planning and attempt was 10.7% (95% CI: 8.6–12.9%), 5.1% (95% CI: 3.6–6.6%) and 3% (95% CI: 1.9–4.3%), respectively. A longer duration of homelessness (1–2 years) (AOR = 2.244, 95% CI: 1.447–3.481), stressful life events (AOR = 1.655, 95% CI: 1.132–2.418) and the stigma associated with homelessness (AOR = 1.629, 95% CI: 1.149–1.505) were significantly associated with suicidal behaviour.

Conclusion
The results of our study indicate that suicide is a serious public health problem among homeless young people in southern Ethiopia. We have found associations between suicidal behaviour and stressful events, homelessness lasting for one to two years and stigma. Our study suggests that policymakers and programme planners need to develop a strategy for preventing, detecting and managing suicidal behaviour among street-dwelling homeless young adults, a vulnerable and understudied population. A community-based suicide prevention campaign is also essential for street-dwelling homeless young people in Ethiopia.
A new method to reconstruct the 3D ground surface temperature from aerial TIR and visible images: application to the active crater of Aso volcano, Japan
Authors: Nashimoto Subaru , Yokoo Akihiko 
Published: April 2023

AbstractAt active volcanoes, the surface temperature and its spatial distribution can indicate changes in the underlying magmatic and hydrothermal system. Surface temperature monitoring has been widely performed using thermal remote sensing with thermal infrared (TIR) cameras. One of the drawbacks of this method is that the unknown viewing orientation of TIR images inhibits quantitative evaluations of the spatial extent and distributions of thermal anomalies. Therefore, many studies have performed 3D temperature-field reconstructions by processing TIR images photogrammetrically. However, these studies have not included the correction of TIR wave atmospheric attenuation and viewing-angle variation in emissivity in the reconstruction procedure, which can result in significant temperature misestimation. We propose a simple method that incorporates the correction into the reconstruction process, which can improve the estimation of surface temperature, especially in rugged terrains. We demonstrate our method for the active crater of Aso volcano in Japan. We create digital elevation models by applying the Structure from Motion–Multi-view Stereo algorithm to aerial visible images taken at the same time as the TIR images and then project the TIR images onto the DEM with the direct georeferencing method. The correction is carried out using the geometric parameters acquired in the process. We create 1-m resolution orthorectified thermal images of the crater on two separate dates (18 August 2020 and 16 March 2022) and calculate the heat discharge rates from steaming grounds and a volcanic lake. We find that the heat discharge rate from the fumarolic field on the south crater wall showed a sevenfold increase after the phreatic explosions that occurred on 14 and 20 October 2021.
Graphical Abstract
Successful omental flap coverage repair of a rectovaginal fistula after low anterior resection: a case report
Authors: Kuhara Yuta , Hotei Hiroshi , Hashimoto Tatsunori , Seo Shingo , Amioka Ai , Murao Naoki , Kuwada Aki , Nakashima Akira , Sakabe Ryutaro , Tahara Kou 
Published: April 2023

Abstract
Background
Rectovaginal fistula (RVF) is a troublesome and refractory complication after low anterior resection (LAR) for rectal cancer. An omental flap repair was performed for the RVF caused due to Crohn’s disease and childbirth trauma. However, there are few cases of an omental flap repair for RVF after LAR. Herein, we present a successfully repaired case of RVF by omental flap coverage after LAR for rectal cancer.

Case presentation
A 50-year-old female patient with advanced rectal cancer underwent laparoscopic LAR with double-stapling technique anastomosis and achieved curative resection. She complained of a stool from the vagina and was diagnosed with RVF on the postoperative day (POD) 18. Conservative therapy was ineffective. We performed laparoscopic fistula resection and direct closure of the vagina and rectum, designed the omentum that could reach the pelvis, repaired RVF by omental flap coverage, and performed transverse colostomy on POD 25. She was discharged on initial POD 48. Seven months after the initial operation, colostomy closure was administered. There was no recurrence of RVF found 1 year after the initial operation.

Conclusions
The patient achieved an omental flap coverage for RVF. We successfully performed the omental flap coverage repair in patients with RVF after the leakage of LAR. An omental flap may become an alternative treatment for muscle flap or an effective treatment for RVF.
Effects of probiotic supplementation with weight reducing intervention on anthropometric measures, body composition, eating behavior, and related hormone levels in patients with food addiction and weight regain after bariatric surgery: a study protocol for a randomized clinical trial
Authors: Ghafouri-Taleghani Fateme , Abiri Behnaz , Zamanian Ali , Saidpour Atoosa 
Published: April 2023

Abstract
Background
One of the unfortunate events after bariatric surgery is the weight regain, which occurs in some patients. Food addiction is an eating disorder related to the brain-intestinal axis and can be effective in weight regain after bariatric surgery. In addition, the gut microbiome plays a vital role in eating behaviors, including food addiction. So, this study will aim to evaluate the effects of probiotic supplementation with a weight-reducing diet and cognitive behavioral therapy on anthropometric measures, body composition, eating behavior, and related hormone levels, leptin, oxytocin, and serotonin, in patients with food addiction and weight regain after bariatric surgery.

Methods
We will carry out a triple-blinded randomized clinical trial for 12 weeks to evaluate the effect of probiotic supplementation with a weight-reducing diet and cognitive behavioral therapy on anthropometric measures, body composition, eating behavior, and related hormone levels including leptin, oxytocin, and serotonin, in patients with food addiction and weight regain after bariatric surgery.

Discussion
Based on the available evidence, probiotic supplementation by modifying the intestinal microbiome can improve food addiction and subsequent weight loss.

Trial Registration
Iranian Registry of Clinical Trials IRCT20220406054437N1 Registered on 2022–06-01.