bluuebunny/crossref_metadata_2025_split huggingface dataset:
Free Triiodothyronine predicts the risk of developing Diabetic Kidney Disease
Authors: Li Weihong , Yang Zhi , Li Shengjian , Jiang Shanshan , Hu Wan , Wan Zhenying , Tu Ping , Duan Peng 
Published: February 2023

Abstract
Purpose Low levels of Free Triiodothyronine (FT3) are associated with poor survival in chronic kidney disease, and the aim of this study was to further assess the relationship between changes in FT3 levels and renal damage in patients with type 2 diabetes based on glomerular and tubular markers. Methods We retrospectively studied 452 type 2 diabetic patients, measured glomerular damage markers (UACR, eGFR) and tubular damage markers (NAG/Cr,β2-MG), analyzed the relationship between FT3 and renal damage by logistic regression models, and plotted restrictive cubic splines. Results 41.6% of subjects had diabetic kidney disease (DKD), and the prevalence of DKD decreased progressively with increasing FT3 levels in the third quartile. Spearman correlation analysis showed that FT3 was negatively associated with UACR, NAG/Cr and β2-MG, while eGFR was positively associated with FT3. Multifactorial analysis, after adjusting for relevant confounders, revealed that compared with the lowest quartile of FT3, the highest quartile reduced the risk of developing urinary albumin (OR = 0.499,95% CI:0.289–0.856), moderate to severe impairment of glomerular filtration rate (OR = 0.106,95% CI:0.032–0.354), renal tubular marker β2 -MG positive (OR = 0.516,95% CI:0.299 to 0.883) and the risk of DKD occurrence (OR = 0.450,95% CI:0.260 to 0.774). In the sample model, FT3 levels below 4.39 pmol/L were associated with an increased risk of glomerular tubule injury and DKD occurrence. Conclusion FT3 is closely associated with glomerular tubular injury and is a protective factor. As FT3 levels (< 4.39 pmol/L) decrease, the risk of developing DKD becomes higher, and FT3 can be used as an independent predictor of developing DKD.
Nature-skin-based multi-responsive controlled-release electroactive scaffolds for integrated melanoma postoperative relapse prevention and wound therapy
Authors: Zheng Manhui , Wang Xuechuan , Zhang Jiamin , Yue Ouyang , Zhang Jingyu , Bai Zhongxue , Jiang Huie , Wen Ling , Liu Xinhua 
Published: February 2023

Abstract
Owing to the high recurrence rate of melanoma, pursuing multifunctional biomaterials with adjuvant therapy functions for full-scale postoperative relapse prevention, wound healing, and real-time postoperative surveillance is an ideal strategy for integrated melanoma treatment. Emerging conductive biomaterials in bioelectronics have exhibited enormous potential as next-generation wound-healing biomaterials. Herein, an intelligent nature-skin-derived multi-responsive controlled-release electroactive scaffold (PADM-MX-Ag-Si@Dox) was nano-engineered by incorporating the functional building blocks of porcine dermal matrix and MXene nanosheets, silver nanowires (AgNWs), and on-demand multiple-response drug-loading microspheres (TSOHSiO2@Dox). The proposed TSOHSiO2@Dox endowed PADM-MX-Ag-Si@Dox with the controlled release property of Dox. Interestingly, the Dox release behavior of the scaffold could be regulated by integrated temperature, pH, and ancillary electrical stimulation. Electrical stimulation simultaneously induced anticarcinogen escape from PADM-MX-Ag-Si@Dox and accelerated wound healing. Meanwhile, PADM-MX-Ag-Si@Dox exhibited outstanding antimicrobial and electrophysiological activities, providing a strong foundation for wound healing elevation and real-time postoperative tumor surveillance. Comprehensive in vitro and in vivo experiments proved that PADM-MX-Ag-Si@Dox in synergy with electrical stimulation achieved anticipatory integration of melanoma postoperative relapse prevention, wound healing, and real-time postoperative surveillance, demonstrating an effective therapeutic strategy for postoperative cancer adjuvant therapy and leading to new precision medical care.
Prevention of invasive ventilation (PRiVENT) - a prospective, mixed-methods interventional, multicentre study with a parallel comparison group: study protocol
Authors: Michels Julia D. , Meis Jan , Sturm Noemi , Bornitz Florian , Schumann Selina , Weis Aline , Neetz Benjamin , Bentner Martina , Forstner Johanna , Litke Nicola , Wensing Michel , Erdmann Stella , Grobe Thomas , Frerk Timm , Kempa Axel , Neurohr Claus , Schneider Armin , Müller Michael , Herth Felix JF , Szecsenyi Joachim , Trudzinski Franziska C. 
Published: February 2023

Abstract
Background
Invasive mechanical ventilation (IMV) is a standard therapy for intensive care patients with respiratory failure. With increasing age and multimorbidity the number of patients rises, who cannot be weaned from IMV, leading to impaired quality of life and high costs. In addition, human resources are tied up in the care of those patients.
Methods
The PRiVENT intervention is a prospective, mixed-methods interventional, multicentre study with a parallel comparison group selected from insurance claims data of the health insurer Allgemeine Ortskrankenkasse Baden-Württemberg (AOK-BW) conducted in Baden-Württemberg, Germany, over 24 months. Four weaning centres supervise 40 intensive care units (ICUs), that are responsible for patient recruitment. The primary outcome, successful weaning from IMV, will be evaluated using a mixed logistic regression model. Secondary outcomes will be evaluated using mixed regression models.
Discussion
The overall objective of the PRiVENT project is the evaluation of strategies to prevent long-term IMV. Additional objectives aim to improve weaning expertise in and cooperation with the adjacent Intensive Care Units.
Trial registration number:
This study is registered at ClinicalTrials.gov (NCT05260853).
Analysis of risk factors of type 2 diabetes mellitus patients complicated with hypertension and establishment of prediction model
Authors: 贯中 田 , Muhadasi Tuerxunyiming , Li Shan , Wang Wei 
Published: February 2023

Abstract
Purpose
To analyze the risk factors of adult patients with type 2 diabetes mellitus complicated with hypertension in Jiangsu province, and establish an individualized risk prediction model of hypertension.
Methods
We analyzed 485 newly diagnosed patients with type 2 diabetes mellitus from 2020 to 2021 in Lianshui County People's Hospital, Huai'an City, Jiangsu province, China. According to the ratio of 7:3, they were randomly divided into training group and validation group. After primary screening by univariate analysis, the predicted variables were determined by multivariate analysis. The nomogram model of hypertension was constructed and evaluated by receiver operating characteristic curve (ROC curve), calibration curve and decision curve analysis(DCA).
Results
This study investigated 485 people, and the prevalence of hypertension was 56.90%. Residential area, systolic blood pressure, family history of diabetes and uric acid are independent risk factors of hypertension among adults in Jiangsu province (P < 0.05). The area under ROC curve (AUC) of hypertension risk prediction model in training group and validation group were 0.7401 and 0.7392, respectively, and the calibration curves showed excellent consistency. DCA shows that the training group shows a great positive rate of return in the risk range of 13%~57% and 58%~100%.
Conclusions
The prediction model based on the related risk factors of hypertension among adult residents in Jiangsu province has excellent accuracy and clinical application value. It can provide a more intuitive way to assess the risk of diabetic patients with hypertension, and has guiding significance for the prevention and treatment of hypertension.
Exploring the Effectiveness of Virtual Reality Role-Playing in Debating Repatriation of Artworks in Active Learning Art History Classes
Authors: Hutson James , Olsen Trent 
Published: February 2023

Abstract
The calls for the repatriation of looted or stolen artworks to host countries continues to grow. With the return of the Benin Bronzes in 2022, renewed calls for the return of the Elgin Marbles from the British Museum have amplified. At the same time, the death of Queen Elizabeth II raises further debate over repatriation and the lingering effects of colonialization. Nevertheless, effectively facilitating such a debate in class has increased in challenge with the lingering effects of the pandemic, the resistance of Gen Z to groupwork and interaction, and elevated levels of anxiety reported among college-age students. One approach that has seen success in face-to-face classes with regards to successful facilitating of debates is the active learning strategy of role playing. Role playing games (RPGs) have the ability to allow students to take on the persona of another to avoid directly confronting the thoughts and opinions of others. At the same time, this approach has been limited only to synchronous activities. This study seeks to investigate the applicability of role-playing within a virtual learning environment (VLE) and whether the control over individual student appearance with avatars and being immersed in a relevant historical environment improves student engagement, immersion, presence, and learning outcomes. Results demonstrate a positive correlation between the activities and understanding diverse perspectives, the ethics involved in cultural repatriation, and lowered anxiety and improved comfort in engaging in groupwork.
Cell-specific and shared enhancers control a high-density multi-gene locus active in mammary and salivary glands
Authors: Hennighausen Lothar , Lee Hye Kyung , Willi Michaela , Liu Chengyu 
Published: February 2023

Abstract
Regulation of high-density loci harboring genes with different cell-specificities remains a puzzle. Here we investigate a locus that evolved through gene duplication1 and contains eight genes and 20 candidate regulatory elements, including a super-enhancer. Five genes are expressed in mammary glands and account for 50% of all mRNAs during lactation, two are salivary-specific and one has dual specificity. We probed the function of eight candidate enhancers through experimental mouse genetics. Deletion of the super-enhancer led to a 98% reduced expression of Csn3 and Fdcsp in mammary and salivary glands, respectively, and Odam expression was abolished in both tissues. The other three casein genes were only marginally affected. Notably, super-enhancer activity requires the additional presence of a distal Csn3-specific enhancer. Our work identifies an evolutionary playground on which regulatory duality of a multigene locus was attained through an ancestral super-enhancer active in mammary and salivary tissue and gene-specific mammary enhancers.
Beneficial effects of Chinese patent medicine ZhengQi tablet on treating the mild COVID-19
Authors: Chen Bowu , Zheng Wenlan , Yan Xiaji , Zhang Jue , Shi Lei , Zhou Wei , Zhang Xin , Li Man , Gao Yueqiu 
Published: February 2023

Abstract
Objectives:
This study aimed to assess the effectiveness of a Chinese patent medicine called ZhengQi tablet in the treatment of mild COVID-19 patients.
Methods:
A prospective cohort trial was carried out in the mobile cabin hospital of City Footprint Hall in Shanghai (ChiCTR2200058693). A total of 800 mild COVID-19 patients were enrolled in this clinical trial and assigned to receive 7 days of treatment with ZhengQi tablet (ZQT group) or TCM placebo (control group) by oral administration per day.
The nucleic acid conversion rate of SARS-CoV-2 was the percentage of subjects who got two negative results of Nucleic Acid Amplification Tests (NAATs) at a 24-hour interval out of the total number. The primary clinical indicators included the nucleic acid conversion rate of SARS-CoV-2 and the incidence rate of common COVID-19. The secondary ones, including the time of negative conversion of SARS-CoV-2 RNA and the hospitalization duration were evaluated.
Results:
A total of 850 mild COVID-19 patients were recruited, and 800 patients were qualified to undergo the clinical trial, with 423 patients assigned for ZQT and 377 patients for TCM placebo. Finally, 390 patients in the ZQT group and 368 patients in the control group completed the follow-up assessments. The nucleic acid conversion rates in ZQT group at 2-day, 3-day and 4-day post-treatment were higher than that in the control group (27.9%vs.9.2%, P<0.001; 38.2%vs.16.6%, P<0.001; 45.4%vs.36.1%, P=0.010). There were no patients who developed into a severe disease. The median time of negative conversion in ZQT group was higher than that in the control group (4[2-6]vs.5[4-6]days, P=0.001). The median hospitalization durations were not different between ZQT group and the control group (5[3-7]vs.6[5-6]days , P=0.065). In terms of the improvement of clinical symptoms, the difference in diarrhea between two groups was statistically significant (100.0%vs.83.3%, P=0.026). There was no significant difference in the improvement of other clinical symptoms. No serious adverse events were reported in both groups.
Conclusions:
ZhengQi tablet showed beneficial effectiveness in treating patients with mild COVID-19 viaimproving the nucleic acid conversion rate of SARS-CoV-2 and shortening the time of negative conversion.
Exploration of photoprotective and antibiotic activity of 20 wild Polypodiaceae ferns from Costa Rica
Authors: Salazar-Chacon Yaclyn , Gutierrez-Bolaños Maria José , Padilla-Cordero Jimena , Vidaurre-Rodriguez Camilo , Carvajal-Miranda Yendry , Rojas-Alvarado Alexander , Rodríguez-Rodríguez Jorengeth Abad , Jimenez-Bonilla Pablo , Rodriguez-Rodriguez Gerardo , Alvarez-Valverde Victor 
Published: February 2023

Abstract
Skin disorders affect millions of people all over the world. There are limited options to treat dermal illnesses such as vitiligo, psoriasis, and atopic dermatitis (eczema). Central American ferns are a potential source of bioactive metabolites against those diseases. Currently, Polypodium leucotomos Poir. (Phlebodium aureum (L.) J. Sm. synonym) is the only one being commercially utilized for this purpose. In this work, we evaluated the concentration of the skin bioactive compounds: quinic and chlorogenic acid, in the extract of 20 wild ferns from Costa Rica. We also evaluated the antimicrobial capabilities of the raw extracts of wild ferns and the sun protection factor (SPF) of the extracts. We found 19 out of 20 have either an important concentration of the compounds mentioned above or antimicrobial properties. Also, most samples result in higher SPF than P. aureum’s rhizome. We also have studied the fern acclimatization, at different shading conditions, finding a significant influence of the culturing conditions on metabolite production. After acclimatization. So far, we demonstrate that various ferns included in this study are a potential source of treatments for skin conditions.
Effect of tool speed on FLC curve position for DX56D
Authors: Novák Vít , Tatíček František 
Published: February 2023

Abstract
Experimentally determined forming limit curves (FLC) are often used to evaluate formability in sheet metal forming. These curves represent the limits of the formability of the material. The most widely used procedure for the experimental determination of the breaking point of sheet metal is formulated in EN ISO 12004-2. The FLC curve was measured for material DX56D + Z100-M-C-O using the Nakajima test on a BUP 600 universal test machine. The test setup and sample shape correspond to EN ISO 12004-2 except for the punching speed, which was increased from 2 mm/s to 10, 14, and 17 mm/s against the conditions defined in the standard. A non-contact optical system ARAMIS from GOM was used to measure the deformation. A stochastic pattern was applied to the samples to ensure the quality of the measurements, and the Erichsen adhesion test was performed. To determine other properties of the material, the microstructure of the material in the fracture area was observed in the metallographically prepared samples. During analysis, the microstructure was monitored with regard to the state of deformation (deep drawing, plane deformation, and more axial stress). Observations were made on samples with an etched structure using an optical microscope and an electron microscope, where, among other things, the distribution of particles, their size, and chemical composition were observed.
Monkeypox virus: an ongoing global outbreak hitting the non-endemic countries - a comprehensive review.
Authors: Nisar Hira , Saleem Omer , Sapna FNU , Sham Sunder , Perkash Raja Sandeep , Anjali FNU , Mehreen Ansa 
Published: February 2023

Abstract
Monkeypox is a rare zoonotic DNA virus with lineage from the Poxviridae family, Chordopoxvirinae subfamily, and Orthopoxvirus genus. (1) With a previous history of controlled and contained occasional outbreaks of the virus, currently a widely erupted outbreak of monkeypox with progressively rising numbers has been reported since May 2022 in multiple countries of the western hemisphere that are not historically endemic for this infection, particularly the UK and EU countries. The global cessation of smallpox vaccination has been hypothesized to cause the rise in monkeypox infections in recent years. (5) (7) Monkeypox like any other viral infection commences with prodromal symptoms; a maculopapular rash with centrifugal distribution usually follows. (16) (9) Polymerase Chain Reaction (PCR) confirms the diagnosis. (16) Transmission in humans is possible through infected animals or humans. (5) (27) In the ongoing 2022 outbreak, monkeypox virus has been undergoing novel mutations at an alarming rate. (56) Treatment options for monkeypox is an area that still requires extensive research, the utility of certain antiviral medications in treating monkeypox infection is currently being explored but is still controversial and debatable.
Genetic variability and population structure of Agropyron desertorum accessions from Iran based on ISSR assay
Authors: Maleki Hamid Hatami , Mohammadi Reza , Arshad Mousa , Hasanzadeh Mina , Rafiee Maryam 
Published: February 2023

Abstract
The genus Agropyron as forage plant has several species which represented resistance against environmental stresses. DNA markers possessed a key role in assessment of plant germplasm and parental selection. In this regard, the genetic diversity of 34 A. desertorum accessions was studied using 28 ISSR primers. A total of 448 loci were amplified in A. desertorum genome that 402 (90%) out of them were polymorphic. The primer (AG)8YC produced the greatest number of polymorphic fragments while the primer (AC)8YA produced the lowest number. The number of effective alleles ranged between 1.26 and 1.57. The highest value of Shanon index was belonged to primer (GA)8T while the highest value of Nei index (0.33) was detected for primers (GA)8T, (TC)8C, and (GACA)4. Primer (GA)8GCC with PIC = 0.40 was the best informative marker in evaluation of A. desertorum genetic diversity. Genetic similarity among studied accessions was between 0.45 (G03 and G17) and 0.80 (G01 and G11). Using Bayesian and WPGMA classification algorithms, the A. desertorum germplasm was divided into two major subgroups (Red and Green) consisted of 13 and 17 accessions and also admixture accessions. Late mature accessions were distinguished from early mature accessions and mostly localized in Green subgroup. The Red subgroup had more private allele as well as heterozygosity compared with Green subgroup and therefore had also promising selection potential. Totally, ISSR markers could distinguished early mature genotypes and this is significant for more forage yield. Likewise, the recognized heterotic groups could help breeders to select highly polymorphic genotypes in polycross breeding.
B1 cell-produced anti-phosphatidylserine antibodies contribute to lupus nephritis development via TLR-mediated Syk activation
Authors: Lu Liwei , Ma Kongyang , Du Wenhan , Wang Shiyun , Li Jingyi , Tian Jie , Xing Yida , Kong Xiaodan , Rui Ke , Qin Rencai , Zhu Xiaoxia , Wang Jing , Luo Cainan , Wu Haijing , Xiao Fan , He Lan , zou hejian , Wu Lijun , Lu Qianjin , Liu Dongzhou 
Published: February 2023

Abstract
Autoantibodies produced by B cells play a pivotal role in the pathogenesis of systemic lupus erythematosus (SLE). However, both the cellular source of anti-phospholipid antibodies and their contributions to the development of lupus nephritis (LN) remain largely unclear. Here, we report a pathogenic role ofanti-phosphatidylserine (PS) autoantibodies in the development of LN. In cohort study and mouse models, elevated serum PS-specific IgG levels were detected in SLE patients, especially in those with nephritis, and lupus mice. The deposition of PS-specific IgG was detected in kidney biopsied of lupus nephritis patients. Both transfer of SLE PS-specific IgG and PS immunization triggered lupus-like glomerular immune complex deposition in recipient mice. ELISPOT analysis identified B1a cells as the main cell type for secreting PS-specific IgG in both lupus mice and patients. Adoptive transfer of PS-specific B1a cells accelerated PS-specific autoimmune response and renal damage in recipient lupus mice whereas depletion of B1a cells attenuated lupus progression. In culture, PS-specific B1a cells were significantly expanded upon treatment with chromatin components while blockade of TLR signal cascades by DNase I digestion, inhibitory ODN 2088 or R406 treatment profoundly abrogated chromatin-induced PS-specific IgG secretion by lupus B1a cells. Thus, our study has demonstrated that the novel anti-PS autoantibodies produced by B1 cells contribute to lupus nephritis development. Our findings that blockade of TLR/Syk signaling cascade inhibits PS-specific B1 cell expansion may provide new insight in understanding lupus pathogenesis and may help develop novel therapeutic targets for the treatment of LN in SLE.
A novel mutation in intron 1 of Wnt1 causes developmental loss of dopaminergic neurons in midbrain and ASD-like behaviors in rats
Authors: Tang Ya-Ping , Yongyi Li , Zhu Mingwei , Chen Wen-Xiong , Luo Jing , Li Xin , Cao Yangyang , Zheng Meng , Ma Shanshan , Xiao Zhilan , Zhang Yani , Jiang Linyan , Wang Xiumin , Tan Ting , Li Xia , Gong Qian , Xiong Xiaoli , Wang Jun , Tang Mingxi , Li Mingtao 
Published: February 2023

Abstract
Autism spectrum disorder (ASD) is a group of neurodevelopmental disorders with a strong genetic liability. Despite extensive studies, however, the underlying pathogenic mechanism still remains elusive. In the present study, we identified a homozygous mutation in the intron 1 of Wnt1 via large-scale screening of ASD risk/causative genes and verified that this mutation created a new splicing donor site in the intron1, and consequently, a loss of WNT1 expression. Interestingly, humanized rat models harboring this mutation exhibited robust ASD-like behaviors including impaired ultrasonic vocalization (USV), decreased social interactions, and restricted and repetitive behaviors. Moreover, in the substantia nigra compacta (SNpc) and the ventral tegmental area (VTA) of mutant rats, dopaminergic (DAergic) neurons were dramatically lost, together with a comparable decrease in striatal DAergic fibers. Furthermore, using single-cell RNA sequencing, we demonstrated that the decreased DAergic neurons in these midbrain areas might attribute to a shift of the boundary of the local pool of progenitor cells from the hypothalamic floor plate to the midbrain floor plate during the early embryonic stage. Moreover, treatments of mutant rats with levodopa could attenuate the impaired USV and social interactions almost completely, but not the restricted and repetitive behaviors. Our results for the first time documented that the developmental loss of DAergic neurons in the midbrain underlies the pathogenesis of ASD, and that the abnormal progenitor cell patterning is a cellular underpinning for this developmental DAergic neuronal loss. Importantly, the effective dopamine therapy suggests a translational significance in the treatment of ASD.