bluuebunny/crossref_metadata_2025_split huggingface dataset:
Quercetin Inhibits Ectopic Lesion Formation in Mice by Modulating the MAT2A/PRMT5 Pathway through PPARγ Activation
Authors: Zhang Shun , Zhang Yuan-Yuan , Zeng Qiu-Xia , Wang Li , Li Kong-Xian , Chen Qi 
Published: February 2025

Objective:
This study aimed to examine the impact of quercetin on a mouse model of
endometriosis and elucidate its underlying mechanisms.

Methods:
An endometriosis model was established using C57BL/6 mice, which were divided
into three groups: 1) sham group, 2) model group, and 3) model group treated with daily gavage
administration of 100 mg/kg/d quercetin. After three weeks, mice were euthanized, and histopathological
examination was performed using hematoxylin and eosin (HE) staining. The microstructure
of the lesions was examined using electron microscopy, and the expression level of Sadenosylmethionine
(SAM) was measured using enzyme-linked immunosorbent assay (ELISA).
Additionally, the expressions of related proteins, such as the peroxisome proliferator-activated
receptor-γ (PPARγ) and methionine adenosyl-transferase 2A (MAT2A), were analyzed via Western
blotting. Immunohistochemistry was employed to evaluate the expressions of Ki67, vimentin,
vascular endothelial growth factor (VEGF), and Caspase-1.

Results:
The endometriosis mice model was successfully established and characterized by ectopic
lesions displaying transparent or red vesicular or nodular features with a visible vascular network
on the surface. In the model group, endometrial epithelial hyperplasia exhibited columnar
morphology, increased mesenchymal cell numbers, and regular cell morphology. Conversely, in
the medication group, endometrial stromal cell numbers were sparse, cell morphology was irregular,
and numerous vacuoles were observed in the endometrial tissue, indicative of apoptotic cell
morphology changes. In comparison to the sham group, no statistically significant alterations
were observed in the expression levels of SAM (P > 0.05). Conversely, the expression of PPARγ
exhibited a notable decline. MAT2A, PRMT5, cyclin D1, and C-MYC expressions were increased,
and vimentin, Ki67, VEGF, and caspase-1 expressions were strongly positive, with statistically
significant differences (P < 0.05). In contrast, compared to the model group, the quercetin
intervention group exhibited significantly reduced ectopic lesion weights, increased PPARγ
expression, and significantly reduced protein expression levels of MAT2A, PRMT5, SAM, cyclin
D1, and C-MYC. Furthermore, expressions of vimentin, Ki67, VEGF, and caspase-1 were
weakly positive, with statistically significant differences (P < 0.05).

Conclusion:
Quercetin modulated the transcription of the MAT2A/PRMT5 gene by activating
PPARγ activity, thereby influencing the ectopic implantation and growth of endometrial cells.
Preparation of Polydeoxyribonucleotide Nanoliposomes and their Applicability to Cosmetic Formulations
Authors: He Yifan , Xu Li , Pei Xiaojing , Dong Yinmao , Yang Xiangliang 
Published: February 2025

Introduction:
In this study, a PDRN-loaded nanoliposomal (PDRN-NL) formulation, which exhibited
an average particle size of 125 ± 1 nm, a polydispersity index (PDI) of 0.12 ± 0.02, a zeta potential
of -52.6 ± 0.8 mV, and an encapsulation efficiency of 81.3%. The stability of the PDRN-NL
and its formulations was assessed using the Turbiscan Lab stability analyzer, which predicted a
shelf life of up to three years. Furthermore, the in vitro permeability of the PDRN-NL was evaluated
using the Franz diffusion cell method.

Method:
In this study, a PDRN-loaded nanoliposomal (PDRN-NL) formulation, which exhibited
an average particle size of 125 ± 1 nm, a polydispersity index (PDI) of 0.12 ± 0.02, a zeta potential
of -52.6 ± 0.8 mV, and an encapsulation efficiency of 81.3%. The stability of the PDRN-NL
and its formulations was assessed using the Turbiscan Lab stability analyzer, which predicted a
shelf life of up to three years. Furthermore, the in vitro permeability of the PDRN-NL was evaluated
using the Franz diffusion cell method.

Results:
Results indicated that the cumulative skin permeation of PDRN-NL over 24 hours was
1.22 times higher than that of free PDRN, with skin retention of PDRN-NL being 1.40 times
greater. The in vitro release studies demonstrated that liposomal encapsulation not only enhanced
permeability but also provided a sustained-release effect and improved stability of PDRN.

Conclusion:
Overall, the properties of liposome-encapsulated PDRN were significantly enhanced,
presenting a novel solution for the incorporation of PDRN in the development of skincare
products.
DNMT3A Deficiency Reduces DNMT3B Gene Methylation and Contributes to Whole-Genome Transcription Alterations in HEK293 Cells
Authors: Zhang Mengxiao , Wang Jiaxian , Qi Gen , Xie Lanfeng , Tian Qiuxiang , Yang Hui , Feng Lei , Zhu Nan , Pan Xingchen , Zhu Jianwei , Hu Jianjun , Chen Peng , Lu Huili 
Published: February 2025

Introduction:
DNA methylation is an important epigenetic modification associated
with transcriptional repression and plays key roles in normal cell growth as well as oncogenesis.
Among the three main DNA methyltransferases (DNMT1, DNMT3A, and DNMT3B), DNMT3A
mediates de novo DNA methylation. However, the general effect of DNMT3A on cell proliferation,
metabolism, and downstream gene regulation is still to be unveiled.

Method:
In this study, we successfully created DNMT3A-deficient HEK293 cells with frameshift
mutations in the catalytic domain using CRISPR/Cas9 technology. The DNMT3A deficient cells
showed a 21.5% reduction in global DNA methylation levels, leading to impaired cell proliferation
as well as a blockage of MAPK and PI3K-Akt pathways in comparison with wild-type cells.

Result:
RNA-seq analysis demonstrated that DNMT3A knockout resulted in the up-regulation of
genes and pathways related to cell metabolism but down-regulation of those involved in ribosome
function, potentially explaining the growth and signaling pathways inhibition. Furthermore,
DNMT3A ablation reduced DNMT3B gene methylation, explaining the down-regulated profiles of
genes.

Conclusion:
Our findings suggest a complex epigenetic regulatory role for DNMT3A, and the
compensatory upregulation of DNMT3B in response to DNMT3A deficiency warrants further investigation
to be validated in future studies.

conclusion:
Our findings suggest a complex epigenetic regulatory role for DNMT3A, and the compensatory upregulation of DNMT3B in response to DNMT3A deficiency warrants further investigation to be validated in future studies.
Hepatic Fibrosis Predicts the Prognosis of Patients with Acute Ischemic Stroke Through the Mediation of Cardioembolism
Authors: Zhao Mingyue , Huang Jiexi , Zeng Tian , Zhang Minyue , Huang Jiaqi , Gao Yufan , Xie Haobo , Li Shengqi , Chen Yilin , Xu Jiahan , Wang Yanchu , Lin Shenyi , Weng Yiyun , Chen Guangyong 
Published: February 2025

Background:
Hepatic fibrosis, a chronic pathological condition, is associated with adverse
outcomes in stroke patients. Cardioembolism (CE) is a common etiology of stroke, yet the association
between hepatic fibrosis and CE remains understudied.

Aim:
This study aims to investigate the association between hepatic fibrosis and CE-induced stroke,
as well as its impact on stroke patient prognosis.

Methods:
This retrospective study included 344 acute ischemic stroke (AIS) patients who underwent
thrombolytic therapy. Hepatic fibrosis was assessed using the Fibrosis-4 (FIB-4) index and the Aspartate
Aminotransferase-Platelet Ratio Index (APRI). Mediation analysis examined the role of CE in
the association between hepatic fibrosis and 3-month functional outcomes.

Results:
Among 344 patients, 319 were classified using the Trial of Org 10172 in Acute Stroke
Treatment criteria. Severe fibrosis (FIB-4 ≥ 2.01) was observed in 131 patients (38.08%), and CE
was identified in 79 patients. FIB-4 was an independent predictor of CE (OR: 2.038, 95%CI: 1.507-
2.757, p < 0.001) and poor 3-month functional outcome (OR: 1.477, 95%CI: 1.103-1.978, p = 0.009)
after adjusting for confounders. The effect of FIB-4 on poor 3-month functional outcomes was partially
mediated by CE, with a mediation proportion of 30.63%.

Conclusions:
Hepatic fibrosis is a significant predictor of short-term functional outcomes in AIS,
particularly cardioembolic stroke. The association between hepatic fibrosis and stroke outcomes is
partially mediated through CE. These findings highlight the importance of assessing hepatic fibrosis
in stroke patients, particularly those with CE etiology.
Identification of Disease-Protein Associations from a Heterogeneous Network Using Graph Embedding and Sample Screening Algorithms
Authors: Li Zhanchao , Liu Yuqi , Xie Yun , Wang Yan 
Published: February 2025

Background:
Identifying disease-protein associations is a key step in treating disease,
understanding pathomechanisms, and developing drugs. Although experimental methods can be
used to identify disease-protein associations, they are often time-consuming, laborious, and expensive.
Therefore, there is a strong need to develop theoretical computational methods to identify potential
disease-protein associations.

Objective:
This work aimed to study the effect of the graph embedding algorithm and reliable negative
sample screening methods on predicting disease-protein association.

Methods:
In our study, information on disease similarity, disease-protein association, and proteinprotein
interaction was used to construct a heterogeneous network, including protein-protein interaction
subnetwork, disease similarity subnetwork, and disease-protein association subnetwork.
Then, a graph embedding algorithm was utilized to obtain network node features to characterize the
disease-protein relationships. The support vector data description algorithm was applied to screen
the reliable negative samples. Finally, random forest algorithm was employed to construct a model
for identifying potential disease-protein associations.

Results:
The present method achieved an accuracy of 94.55%, a specificity of 98.49%, a precision
of 98.36%, a Matthew's correlation coefficient of 0.8938, an area under the receiver operating characteristic
curve of 0.9815, and an area under the precision-recall curve of 0.9591, based on a constructed
benchmark dataset and a 10-fold cross-validation test. Results from a series of nonredundant
datasets and an independent test dataset showed our method to be robust for data redundancy
and that it can accurately identify disease-related proteins, protein-related diseases, and potential
disease-protein associations. Based on the constructed model, the large-scale prediction
study identified more than 1.7 million potential disease-protein association pairs with a probability
greater than 99%. The top five predicted disease-protein association pairs were further confirmed
by literature and molecular docking simulations.

Conclusion:
Extensive experimental results showed that the proposed method can effectively identify
potential disease-protein associations. It is expected that the current method can help not only in
understanding disease mechanisms at the protein level, but also in discovering new protein targets
and potential small molecule drugs.
Design, Synthesis, and Biological Evaluation of Triazine-4-Thiazolidinone Hybrid Molecules as Modulator of Breast Cancer
Authors: Debnath Rabin , Sharma Kalicharan 
Published: February 2025

Background:
Breast cancer is the most prevalent cancer among women globally, characterized
by the uncontrolled growth of breast cells, and remains a leading cause of cancer-related
morbidity and mortality. It can occur in both men and women, though it is significantly rarer in men.
The multifactorial nature of breast cancer involves genetic mutations, hormonal influences, and complex
cellular signalling pathways. The disease is typically classified into different subtypes based on
hormone receptor status, which influences treatment decisions. Early detection through regular
screening, such as mammograms, and awareness of symptoms significantly improve prognosis.
Treatment options vary based on the stage and type of breast cancer and may include surgery, radiation
therapy, chemotherapy, hormone therapy, and targeted therapy.

Objective:
We aimed to design novel compounds based on reported active pharmacophoric features
and validate them through molecular modelling. These designed compounds were then synthesized
and characterized. Finally, a biological evaluation of the synthesized compounds was performed to
assess their efficacy.

Method:
Thirty compounds were designed based on a literature survey. Out of these compounds,
twelve compounds were found good on the docking studies, and these twelve new derivatives (RD
01-12) were synthesized and subjected to in silico, in vitro (EGFR assay), and ADMET profiling to
identify the most potent compound.

Result:
All 12 compounds were synthesised and characterised. Out of 12 compounds, RD-09
emerged as the most potent enzymatic assay with an IC50 value of 1.21 ± 0.03 μM, confirmed by
docking studies; it possessed a docking score of -7.302 against the EGFR receptor. These compounds
were further characterized using IR, 1H NMR, and mass spectrometry.

Conclusion:
Based on pharmacophoric features, twelve triazine-4-thiazolidinone derivatives (RD 01-
12) were designed, synthesized, and evaluated for their potential as EGFR-2 inhibitors, specifically
targeting triple-negative breast cancer (TNBC). Among 12 synthesised compounds, compound RD-
09 demonstrated the most significant activity with an IC50 value of 1.21 ± 0.03 μM. Docking studies
further supported its binding interaction with the catalytic domain of the EGFR receptor. The combined
results from in vitro, in silico, and ADMET profiling suggest that RD-09 holds promise as a
leading compound for further development in the treatment of TNBC.
A Cross-sectional Study on Knowledge, Perception, and Willingness Among Saudi Population Towards Stem Cell Treatment and Banking:
Associated Factors and their Predictive Abilities
Authors: Aslam Nousheen , Alanzi Rahaf Fares , Alobaid Zainab Mohammed , Alhumood Jumanah Basem , Almustafa Nouf Abdullah , Banu Nuzhat , Ali Mohammad Daud , Ghosn Sherihan Ahmed , Ahmed Wasim , Ahmed Ayaz 
Published: February 2025

Background:
This study aimed to assess the knowledge, perception, and willingness of
the Saudi population towards stem cell treatment and banking, the associated factors, and their predictive
abilities.

Methods:
A cross-sectional study was conducted from September to December 2022 in Saudi Arabia
using a structured bilingual, self-administered online survey to collect sociodemographic information
and determine the knowledge and understanding, perception, and willingness of the general population.
Bloom’s cut-off points were used to distribute the scores into three categories, namely strong
(80-100%), moderate (60-79%), and weak (<59%). Descriptive statistics were used to assess each
domain, while t-tests, ANOVA, and binary logistic regression were used to assess factors influencing
each domain and their predictive abilities.

Results:
The study conscripted 440 respondents, mainly females (70%) aged 18-24 years (56.1%),
mostly single (44.3%), Saudi nationals (89%), and college graduates (56.6%). Social media (53.4%)
was the primary source of information. 77.95% of respondents exhibited a low level of knowledge.
Females, Saudi nationals, respondents with Islamic beliefs, college graduates, and those who received
information from family physicians and social media had significantly better knowledge.
50.68% of respondents showed a strong perception. Non-Saudi participants, those who received information
from family and friends, and respondents with a high prevalence of hypertension and diabetes
in their families had a better perception. However, only 21.59% showed strong willingness
toward stem cell treatment and banking, including Saudi nationals and college graduates. Gender
was found to be a significant predictor for better knowledge and perception, while no sociodemographic
variables significantly predicted willingness.

Conclusion:
This study emphasizes the need for increased awareness, educational campaigns, and
targeted strategies considering various socio-demographic factors to improve the knowledge, perception,
and willingness of the general population toward stem cell treatments and banking in Saudi
Arabia.
Molecular Detection of Ureaplasma urealyticum and Enterococcus faecalis in the Seminal Fluid and their Relationship with Semen Quality in Healthy and Infertile Men in Shiraz, Iran (2021-2022)
Authors: Asgari Fahimeh , Motamedifar Mohammad , Arsanjani Amirhossein Akbarpour , Azimi Taher , Mehdinejadiani Shayesteh 
Published: February 2025

Objective:
The present study aimed to carry out the molecular identification of some bacteria in seminal fluid and investigation of their effects on semen quality

Methods:
The research cohort comprised 80 infertile individuals and 80 men with no fer-tility issues. Evaluation of sperm characteristics adhered to the protocols outlined by the World Health Organization. Detection and verification of pathogens were carried out by PCR.

Results:
The prevalence of bacteriospermia in the semen of the infertile group exhibited a noticeable increase compared to the control group (p<0.05). The most abundant species in the semen of infertile men was Ureaplasma urealyticum (7.5%, p<0.05), followed by En-terococcus faecalis (6.25%, p>0.05). However, Streptococcus agalactiae was not found in any of the abnormal samples. In addition, we showed that Ureaplasma urealyticum signif-icantly affected the motility and morphology parameters. But, the presence of Enterococcus faecalis and Streptococcus agalactiae in semen samples of men does not lead to abnormal sperm production. Besides, there was no significant difference between the groups in terms of volume, but there was a significant difference in morphology, count, and total motility (p<0.001).

Conclusion:
Bacteriospermia is linked to modifications in the characteristics of seminal fluid, potentially resulting in a reduction in the fertilization capacity of spermatozoa. Fur-thermore, Ureaplasma urealyticum is correlated with changes in semen properties that could contribute to a decrease in sperm fertilization potential.
Bibliometric Analysis of Single-Atom Catalysis: A Scoping Review
Authors: Shah Muffarih , Majeed Noor , Ali Asif , Hameed Abdul , Rehan Touseef , Shah Nasrullah 
Published: February 2025

Background:
Single-Atom Catalysts (SACs) are heterogeneous catalysts that demonstrate
exceptional efficiency and selectivity due to the use of individual metal atoms at the atomic
scale. The substantial number of patents filed on SACs underscore their commercial and technological
importance, highlighting their potential across various industries. SACs are increasingly applied
in areas such as energy generation, environmental applications, and chemical synthesis, reflecting
their growing scientific and technical importance.

Objectives:
The objective of this study was to conduct a comprehensive evaluation of existing literature
on SACs and the use of bibliometric analysis to identify scientific output and topic patterns of
research on SACs.

Methods:
A bibliometric analysis was performed on 488 papers related to SACs, utilizing the Web
of Science database of data collection. Analysis of Co-occurrence of keywords, trending research
topics, Citation analysis, Publication areas, the five-year record of Publications, and funding sources
were examined using VOS viewer, R software, and Microsoft Excel.

Results:
The analysis indicates a steady growth in publication on SACs in recent years, with China
leading in research output followed closely by the USA. The highlighting of the global impact and
the collaborative nature of SAC research. The study reveals a diverse range of applications and emphasizes
the increasing scientific and technical focus on this subject.

Conclusion:
This study highlights the essential role of SACs in advancing catalytic science and
maps key trends, collaborations, and applications within the field. The bibliometric insights provide
valuable guidance for the researchers, pointing to potential applications in energy storage, environmental
remediation, and sustainable chemical synthesis. Emerging challenges, such as stability,
scalability, and the development of new materials, call for further investigation to unlock the full potential
of SACs. These insights support future innovation and exploration in the expanding field of
SAC research.
Biotransformation of Hydrocortisone by Mucor hiemalis and In silico Study of the Produced Metabolite against 11β-Hydroxysteroid Dehydrogenase Type I
Authors: Nickavar Bahman , Mehmannavaz Maryam 
Published: February 2025

Background:
Microbial biotransformation of steroids is a valuable method for producing active pharmaceuticals or potentially active steroids, and fungi serve as pow-erful biocatalysts in this process. On the other hand, in silico analyses are preliminary yet influential studies for the evaluation of compounds in the drug development process.

Objective:
This study aimed to examine the ability of Mucor hiemalis to biotransform hydrocortisone, predict the potential binding ability of the products to the 11β-hydroxysteroid dehydrogenase enzyme (as a target for the control of diabetes mellitus type 2), causing its inhibition, and assess the products’ pharmacokinetic properties.

Methods:
The incubation of M. hiemalis with hydrocortisone for nine days resulted in the production of a main product in the culture medium. The metabolite was subsequent-ly isolated using chromatographic procedures and its identity was determined by spectro-scopic analysis. Computational binding studies and pharmacokinetic profile prediction of the metabolite were conducted using AutoDock Vina and SwissADME, respectively.

Results:
The biotransformation of hydrocortisone by M. hiemalis produced 11β,17α,20β,21-tetrahydroxypregn-4-en-3-one as the major metabolite. Molecular dock-ing studies demonstrated that the metabolite can interact with functional residues located at the catalytic site of the enzyme in different ways. Furthermore, the results indicated a favorable pharmacokinetic profile of the produced metabolite.

Conclusion:
The current study showed that M. hiemalis may be considered an effective tool for the biotransformation of steroids and the production of potentially bioactive compounds.