bluuebunny/crossref_metadata_2025_split huggingface dataset:
Marine Bacteria: A Source of Novel Bioactive Natural Products
Authors: Zha Xiangru , Ji Rong , Zhou Songlin 
Published: August 2023

:
Marine natural products have great pharmacological potential due to their
unique and diverse chemical structures. The marine bacterial biodiversity and the unique
marine environment lead to a high level of complexity and ecological interaction among
marine species. This results in the production of metabolic pathways and adaptation
mechanisms that are different from those of terrestrial organisms, which has drawn significant
attention from researchers in the field of natural medicine. This review provides an
analysis of the distribution and frequency of keywords in the literature on marine bacterial
natural products as well as an overview of the new natural products isolated from the
secondary metabolites of marine bacteria in recent years. Finally, it discusses the current
research hotspots in this field and speculates on future directions and limitations.
Qingfei Formula Protects against Human Respiratory Syn cytial
Virus-induced Lung Inflammatory Injury by Regulating the M APK
Signaling Pathway
Authors: Sun Ya-Lei , Zhao Pei-Pei , Zhu Cheng-Bi , Li Xin-Min , Yuan Bin 
Published: August 2023

Objective:
Qingfei formula (QF) is an empirical formula that shows good clinical efficacy
in treating human respiratory syncytial virus pneumonia (RSVP). However, the underlying
mechanism remains unclear. This study explores the possible pharmacological actions of QF in
RSVP treatment.

Methods:
We used a network pharmacology approach to identify the active ingredients of QF,
forecast possible therapeutic targets, and analyze biological processes and pathways. Molecular
docking simulation was used to evaluate the binding capability of active ingredients and therapeutic
targets. Finally, in vivo experiments confirmed the reliability of network pharmacology-based
prediction of underlying mechanisms.

Results:
The study identified 92 potential therapeutic targets and corresponding 131 active ingredients.
Enrichment analysis showed that QF downregulated the MAPK signaling pathway and suppressed
the inflammatory injury to the lungs induced by the RSV virus. Molecular docking simulations
demonstrated that the core active ingredients of QF could stably bind to genes associated with
the MAPK signaling pathway. QF had a protective effect against pneumonia in RSV-infected
mice. The QF group exhibited a significant reduction in the levels of inflammatory mediators, interleukin-
6 (IL-6), interleukin-8 (CXCL8, IL-8), and P-STAT3, compared to the RSV-induced
group. The QF group showed remarkably inhibited MAPK1+3(P-ERK1+2) and MAPK8(P-JNK)
protein expression.

Conclusion:
The current study showed that QF downregulated the MAPK signaling pathway,
which inhibited pulmonary inflammation triggered by RSV infection. This study recommends the
appropriate use of QF in the clinical management of RSVP.
Identification of Selective JAK3/STAT1 and CYP34A from Pyrazolopyrimidine Derivatives: A Search for Potential Drug Targets for Rheumatoid Arthritis using In-silico Drug Discovery Techniques
Authors: Faris Abdelmoujoud , Ibrahim Ibrahim M. , Chakraborty Souvik , Al Kamaly Omkulthom , Alshawwa Samar Zuhair , Elhallaoui Menana 
Published: August 2023

Objective:
This study aimed to discover a novel active compound capable of effectively inhibiting JAK3/STAT1 and CYP3A4 using molecular modelling techniques, with the goal of treating autoimmune diseases such as cancer and specifically rheumatoid arthritis. The study involved modelling compounds derived from pyrazolopyrimidine, followed by screening methods to identify the most promising compounds. Moreover, this study seeks to identify potential compounds that can inhibit JAK3/STAT through molecular modelling techniques and validate the stability and affinity of the predicted molecule.

Methods:
Various molecular modelling techniques were employed to identify potential compounds and assess the stability and affinity of the predicted molecule. A pharmacophore hypothesis was developed to obtain crucial information about the experimental series of pyrazolopyrimidine studied, which served as the basis for designing new molecules. Additionally, ADMET was utilized to predict and evaluate the pharmacokinetic properties and potential toxicity of the compound prior to synthesis or utilization. To determine the essential residues involved in the interaction between the molecule and the target JAK3 protein, the covalent docking method was applied. We further validated the binding stability of the JAK3 protein with the ligands ZINC62162141 and Tofacitinib, both of which have been approved by the FDA for JAK3/STAT inhibition., using DFT/B3LYP/6-31G molecular dynamics simulations lasting 1000 ns and MM/GBSA.

Results:
During the study, we identified compounds that displayed notable activity against JAK3/STAT, specifically those containing thiadiazol, oxadiazol, and chlorophenyl groups. Additionally, the pharmacophore model, ADRRR_1, exhibited promising potential for predicting new molecules. The predicted compound, ZINC62162141, demonstrated favourable ADMET properties, including inhibition of CYP3A4. Furthermore, we assessed its binding stability to the target protein and determined its affinity for the protein-ligand complex using MMGBSA.

Conclusion:
The results of this study suggest that the compounds identified have the potential to be promising candidates for inhibiting JAK3/STAT and CYP3A4, offering potential therapeutic benefits for the treatment of rheumatoid arthritis. These findings provide a foundation for subsequent experimental validation and the development of novel drugs in this field.
Recent Advances in Transition Metal Catalyzed Synthesis of
C3-Substitution-free 2-Oxindole Derivatives
Authors: Das Partha Pratim , Das Debapratim 
Published: August 2023

Abstract:
2-Oxindole unit is one of the most important scaffolds found in several alkaloids, natural
products, antitumor agents, pharmaceutically important compounds, etc. Molecules containing the 2-
oxindole moiety were first isolated from the cat claw plant, widely distributed in the Amazon jungle.
It has now been demonstrated that these molecules are present in a wide range of chemicals derived
from plant sources. The capacity of 2-oxindole to be altered by various chemical groups to provide
unique biological activities can be attributed to its function as a chemical framework for creating and
developing biological medications. Since the development of its first synthetic methodology, several
research groups have developed protocols for producing 2-oxindole core and its bioactive derivatives.
These include the traditional method and the transition/non-transition metal-catalyzed pathway
for the synthesis of C3-non-substituted/C3-mono-substituted/C3-di-substituted core. Among those,
C3-substitution-free 2-oxindole core synthesis is quite a challenging task, as C3-centre is very reactive.
Syntheses of C3-substitution-free 2-oxindole cores have been less explored compared to other
substituted 2-oxindole derivatives. In this review article, we have mainly focused on showcasing the
transition metal-catalyzed synthetic methodology for the synthesis of 2-oxindoles with no substitution
at C3-centre.
Atopic March in Children: A Case Report and Review of Current Literature
Authors: Indolfi Cristiana , Klain Angela , Maiello Nunzia , Palumbo Paola , Palladino Eleonora , Dinardo Giulio , Contieri Marcella , Decimo Fabio , del Giudice Michele Miraglia 
Published: August 2023

Background:
The concept of atopic march was introduced about 15 years ago to define the temporal progression of different allergic conditions, from atopic dermatitis and food allergies in earliest childhood to the development of asthma and allergic rhinitis in older children.

Case Presentation:
The authors describe a case report of a young girl who went through the stages of atopic march. The child first showed atopic dermatitis and food allergy in infancy, and, after-wards, she manifested allergic rhinitis and asthma in childhood.

Discussion:
This timeline reflects the atopic march's spatial evolution, beginning with the skin and gastrointestinal tract and progressing to the upper and lower airways.

Conclusion:
In accord with the most recent evidence, rather than being defined in terms of chrono-logical progression, atopic diseases should be investigated as a spectrum of atopic disorders that might take various developmental paths. According to this theory, the use of Component Resolved Diagnosis in atopic children can be beneficial for highlighting and predicting the atopic profile du-ring the earliest years of life before symptoms arise, helping to stratify the clinical risk and preven-ting the onset of the atopic march and severe responses.
Advances in Cell Transplantation Therapy for Limbal Stem Cell Deficiency
Authors: Gui Yujia , He Yuxi , Wang Di , Wang Shurong , Zhang Yan 
Published: August 2023

Background:
Limbal stem cells (LSCs) are essential for maintaining corneal transparency and
ocular surface integrity. Many external factors or genetic diseases can lead to corneal limbal stem cell
deficiency (LSCD), resulting in the loss of barrier and corneal epithelial cell renewal functions. Stem cell
transplantation is one of the primary treatments for LSCD, including limbal transplantation and cultivated
limbal epithelial transplantation. In addition, a variety of non-limbal stem cell lines have been experimented
with for LSCD treatment. Biological scaffolds are also used to support in vitro stem cell culture
and transplantation. Here, we review the mechanisms of corneal maintenance by LSCs, the clinical stage
and surgical treatment of LSCD, the source of stem cells, and the biological scaffolds required for in vitro
culture.

Methods:
This study is a narrative retrospective study aimed at collecting available information on various
aspects of surgical treatments for LSCD. Relevant literature was searched in a range of online databases,
including Web of Science, Scopus, and PubMed from 2005 to March, 2023

Results:
A total of 397 relevant articles were found, and 49 articles with strong relevance to the studies in
this paper were obtained and analyzed. Moreover, 11 of these articles were on the concept of LSCD and
the mechanism of LESCs maintaining the corneal epithelium, 3 articles on the staging and grading of
LSCD, 17 articles on cell transplantation methods and donor cell sources, and 18 articles on scaffolds for
delivering stem cells. We also summarized the advantages and disadvantages of different cell transplantation
methods and the benefits and limitations of scaffolds based on the above literature

Conclusion:
The treatment of LSCD is determined by the clinical stage and whether it involves monocular
or binocular eyes. Appropriate surgical techniques should be taken for LSCD patients in order to reconstruct
the ocular surface, relieve symptoms, and restore visual function. Meanwhile, biological scaffolds
assist in the ex vivo culture and implantation of stem cells.
Identification of Stem Cell-related Gene Markers by Comprehensive
Transcriptome Analysis to Predict the Prognosis and Immunotherapy of
Lung Adenocarcinoma
Authors: Lai Hongzhang , Wen Xiwu , Peng Yukun , Zhang Long 
Published: August 2023

Background:
Cancer stem cells (CSCs) contribute to metastasis and drug resistance to immunotherapy
in lung adenocarcinoma (LUAD), so the stemness evaluation of cancer cells is of great significance.

Method:
The single-cell RNA sequencing (scRNA-seq) data of the GSE149655 dataset were collected
and analyzed. Malignant cells were distinguished by CopyKAT. CytoTRACE score of marker genes in
malignant cells was counted by CytoTRACE to construct the stemness score formula. Sample stemness
score in TCGA was determined by the formula and divided into high-, medium- and low-stemness score
groups. LASSO and COX regression analyses were carried out to screen the key genes related to the
prognosis of LUAD from the differentially expressed genes (DEGs) in high- and low-stemness score
groups and a risk score model was constructed.

Result:
Seven types of cells were identified from a total of 4 samples, and 193 marker genes of 3455 malignant
cells were identified. There were 1098 DEGs between low- and high-stemness score groups of
TCGA, of which CPS1, CENPK, GJB3, and TPSB2 constituted gene signatures. The 4-gene signature
could independently evaluate LUAD survival in the training and validation sets and showed an acceptable
area under the receiver operator characteristic (ROC) curves (AUCs).

Conclusion:
This study provides insights into the cellular heterogeneity of LUAD and develops a new
cancer stemness evaluation indicator and a 4-gene signature as a potential tool for evaluating the response
of LUAD to immune checkpoint blockade (ICB) therapy or antineoplastic therapy.
New Promising Routes in Peptic Ulcers: Toll-like Receptors and
Semaphorins
Authors: Jacob Teresa V. , Doshi Gaurav M. 
Published: August 2023

Abstract:
Peptic ulcers (PU) are one of the commonest yet problematic diseases found to be existing
in the majority of the population. Today, drugs from a wide range of therapeutic classes are
available for the management of the disease. Still, the complications of the condition are difficult to
tackle and the side effect profile is quite a concern. The literature indicates that Toll-like receptors
(TLRs) and Semaphorins (SEMAs) have been under study for their various pharmacological actions
over the past few decades. Both these signalling pathways are found to regulate immunological and
inflammatory responses. Moreover, receptors and signalling molecules from the family of TLRs and
SEMAs are found to have bacterial recognition and antibacterial properties which are essential in
eradicating Helicobacter pylori (H. pylori), one of the major causative agents of PU. Our understanding
of SEMAs, a class of proteins involved in cell signalling, is relatively less developed compared
to TLRs, another class of proteins involved in the immune response. SEMAs and TLRs play
different roles in biological processes, with SEMAs primarily involved in guiding cell migration
and axon guidance during development, while TLRs are responsible for recognizing pathogens and
initiating an immune response. Here, in this review, we will discuss in detail the signalling cascade
of TLRs and SEMAs and thereby understand its association with PU for future therapeutic targeting.
The review also aims at providing an overview of the study that has been into exploring the role
of these signalling pathways in the management of PU.
Characterization of Position Synchronous Control System for Double
Hydraulic Cylinder
Authors: Gao Bingwei , Xu Bin , Zhang Wei , Zheng Lintao 
Published: August 2023

Background:
There are some problems in the synchronous control system of electrohydraulic
servo double hydraulic cylinders, such as uncertainty of system parameters, time variation,
difference between two subsystems, and unbalanced load disturbance during operation. The
above problems cause the output responses of the two cylinders to be inconsistent, resulting in the
situation that the output flows of the two cylinders are not synchronized.

Objective:
Further improve the accuracy of synchronous control while reducing the cost of using
synchronous control system.

Methods:
The AMEsim simulation model of electro-hydraulic servo dual-cylinder synchronous
control system is established, and based on this model, the dynamic characteristics, coupling characteristics,
and synchronization characteristics of the double-cylinder system are analyzed theoretically
and simulated.

Results:
Analyze and verify the influence of various factors on the synchronization of double hydraulic
cylinders, and summarize the influence law of the change of different factors on the output
and synchronization accuracy of the double hydraulic cylinder system.

Conclusion:
This study analyzes the influence of various factors on the synchronization of double
cylinders by adjusting the parameters, and summarizes the influence law of the change of different
factors on the output and synchronization accuracy of the double cylinder system.
Analisis Kemampuan Pemecahan Masalah Matematis Siswa SMA Kelas XI SMAN 1 Bangkinang Kota Ditinjau dari Gaya Belajar
Authors: Syahril Ravina Faradilla , Maimunah Maimunah , Roza Yenita 
Published: August 2023

Penelitian ini bertujuan mendeskripsikan kemampuan pemecahan masalah matematis (KPMM) siswa dan kaitannya dengan gaya belajar siswa khususnya pada materi Sistem Persamaan Linear Tiga Variabel. Penelitian ini menggunakan metode campuran, dengan menggabungkan penelitian kuantitatif dan kualitatif. Subjek dalam penelitian ini adalah siswa kelas XI SMAN 1 Bangkinang Kota Tahun Pelajaran 2021-2022 sebanyak 20 orang. Instrumen yang digunakan adalah tes kemampuan pemecahan masalah matematis yang terdiri atas 5 butir soal dengan 4 indikator KPMM menurut Polya dan angket gaya belajar siswa. Hasil penelitian ini menunjukkan bahwa kemampuan pemecahan masalah siswa pada indikator memahami masalah berada pada kriteria sangat rendah dengan persentase per indikator 40%, indikator merencanakan pemecahan masalah berada pada kriteria sangat rendah dengan persentase 36%, indikator menyelesaikan rencana pemecahan masalah berada pada kriteria sangat rendah dengan persentase 36% dan indikator menafsirkan hasil yang diperoleh berada pada kriteria sangat rendah dengan persentase 22%. Kemampuan pemecahan masalah matematis tidak memiliki kaitan dengan gaya belajar yang dimilikioleh siswa. Setiap siswa dengan gaya belajar apapun memiliki kecendrungan yang sama dalam memecahkan masalah. Berdasarkan hasil penelitian menunjukkan bahwa siswa tidak terbiasa mengerjakan soal-soal non rutin seperti soal-soal pemecahan masalah, sehingga siswa perlu untuk dibiasakan mengerjakan soal-soal non rutin khususnya yang dapat melatih kemampuan pemecahan masalah siswa.
Citation verbs of speaking in Japanese blogs: a case study
Authors: Ермишина Е.С. 
Published: August 2023

В настоящей работе рассматриваются цитационные глаголы «говорения» в блогах на японском языке. Предметом исследования выступают материалы с платформы www.ameba.jp, содержащей блоги (электронные дневники) на бытовые темы.
This paper deals with the citation verbs of speaking in Japanese blogs. The subject of the study is the materials from the www.ameba.jp platform, which contains blogs (electronic diaries) on everyday topics.
Effect of Levels and Time of Nitrogen Application on Yield and Economics of Rice
Authors: Ajmal K. K. , Goverdhan M. , Sridevi S. , Suresh K. 
Published: August 2023

A field experiment was conducted on yield and economics of rice during 2017 at Agriculture Research Institute, Rajensranagar. The study was conducted with 10 treatments and laid out in randomized block design with 3 replications. The soil of the experimental site was clay loam in texture, slightly alkaline in reaction, non-saline, low in organic carbon and available nitrogen, high in available phosphorous and potassium.Among the different time and dose of application of nitrogen, the highest panicle m-2 (309.3), panicle length (23.9 cm), number of filled grains panicle-1 (103.2),number of total grains panicle-1 (118.1), test weight (23.9 g), grain yield (4514 kg ha-1), straw yield (5470 kg ha-1) and B: C ratio (1.99)were recorded highest with application of 160 kg N ha-1 25% each at sowing, 20 DAS, 40 DAS and 60 DAS compared to the other treatments. However, growth recorded with 160 kg in 4 equal splits was found to be on par with the growth in the treatments having 160 and 140 kg N ha-1applied at various splits and with application of 120 kg N ha-1 in 4 equal splits at sowing, 20 DAS, 40 DAS and 60 DAS. The lowest observation wasfoundin the farmer’s practice with the application of nitrogen at 120 kg ha-1 N in 3 splits with 12% at sowing, 44% at 40 DAS and 44% at 60 DAS.
Studies on Genetic Variability and Character Association in Chilli Genotypes (Capsicum annuum L.)
Authors: Singh Sakshi , Joshi A. K. , Vikram Amit , Kansal Sandeep , Singh Sudha 
Published: August 2023

The present investigation was carried out at Experimental Farm of Regional Horticultural Research and Training Station Dhaulakuan, District Sirmour (HP), Dr. YS Parmar University of Horticulture and Forestry, Nauni, Solan, Himachal Pradesh, during Kharif season, 2018 to ascertain extent of variability, heritability and genetic advance as percentage of mean for yield and other horticultural traits of 21 chilli genotypes. The experiment was laid out in RCBD with three replications at spacing of 45cm × 45cm. Analysis of variance indicated significant differences among the genotypes for all the traits studied. RACH-132 exhibited the maximum number of fruits per plant (97.27), fruit weight at edible maturity (4.08 g), green fruit yield plant-1 (280.10 g), red fruit yield plant-1 (275.89 g) and fruit diameter (1.23 cm) while, number of seeds and thousand seed weight was maximum in RACH-121 (100.40, 5.12 g). DKC-8 (0.49%) recorded the maximum capsaicin content. The estimates of phenotypic and genotypic coefficients of variation (PCV and GCV) were moderate to high for all characters studied except days to first red maturity. High heritability coupled with high genetic gain was found for characters like green fruit yield plant-1, red fruit yield plant-1 and capsaicin content which indicates that these characters were under the strong influence of additive gene action and hence simple selection based on phenotypic performance of these traits would be effective.
Effect of Sowing Time and Nutrient Management on Physiological Parameters of Wheat
Authors: Bhayal Lalita , Kewat M. L. , Aakash  , Jha A. K. , Bhayal Divya , Badkul Anamika Jain 
Published: August 2023

A study was undertaken to evaluate the impact of soil application of spent mushroom substrate (SMS) and farm yard manure (FYM) enriched with T. harzianum on pre-emergency damping off (PED) and post-emergence damping off (POED) in chilli and tomato seedlings in nursery beds containing naturally infested soil with Pythium aphanidermatum and Rhizoctonia solani in the experimental field area of Department of Plant Pathology, CCSHAU Hisar during 2018-19, 2019-20and 2020-21.The observations on disease incidence (%) were recorded up to 30 days after germination. A control without soil amendment was also maintained. The maximum damping off was at 22.5, 23.4 and 22.9% in controland minimum at 15.7, 15.8 and 16.6% during 2018–19, 2019–20, 2020–21, respectively in chilliwhen SMS enriched with T. harzianum was applied. The mean of damping off disease incidence was minimum at 16.0% when SMS enriched with T. harzianum was applied and maximum at 22.9% in control. The maximum damping off was at 18.1, 19.4 and 18.7% in control and minimum at 12.2, 12.6 and 14.8% during 2018–19, 2019–20, 2020–21, respectively in tomato when SMS enriched with T. harzianum was applied. The mean of disease incidence (%) was minimum at 13.2% when SMS enriched with T. harzianum was applied and maximum at 18.7% in control.