bluuebunny/crossref_metadata_2025_split huggingface dataset:
Characterization by LC–MS/MS of oxidized products identified in synthetic peptide somatostatin and cetrorelix submitted to forced oxidative stress by hydrogen peroxide: Two case studies
Authors: Datola Antonio , Pistacchio Alessandra , Simone Patrizia , Colarusso Lucia , Melchiorre Maura , Rinaldi Gianluca , Amidi Maryam , Politi Jane , Angiuoni Gabriella 
Published: April 2023

AbstractIn a broader scenario, the forced degradation studies provided by the ICH guidelines for Q1A, Q1B, and Q2B degradation studies allow to know the CQA of the molecule used as a drug product, to determine the appropriate analytical methods, excipients, and storage conditions ensuring the quality of the drug, its efficacy, and patient safety. In this study, we focused our attention on understanding how oxidative stress is performed by H2O2‐impacted small synthetic peptides that do not contain residues susceptible to oxidation such as methionine. Among the amino acids susceptible to oxidation, methionine is the most reactive and depending on the structure of the protein where it is exposed, it tends to oxidize by converting into methionine sulfone or methionine sulfoxide by oxidation of its sulfur atom. Scouting experiments obtained by forced oxidative stress conditions are presented on two small synthetic peptides that do not contain any methionine residues spiked with different amounts of H2O2, and they are analyzed by LC–MS/MS. Less frequent oxidation products than those commonly observed on proteins/peptides‐containing methionine have been characterized on both peptides. The study demonstrated that somatostatin, by means of one residue of tryptophan on the molecule, can generate traces of several oxidized products detected by UPLC–MS. Furthermore, even at a negligible level, oxidation on tyrosine and proline in cetrorelix that does not contain methionine nor tryptophan has been detected by UHPLC–MS/MS. Identification and quantification of oxidized species were achieved by high‐resolution MS and MS/MS experiments. Thus, FDSs undoubtedly aid the evaluation of the CQAs as an important component of the characterization package as recommended by HAs and ICH, facilitating the understanding of unforeseen features of the studied molecule used as drugs.
Abundance and distribution of arbuscular mycorrhizal fungi associated with oil‐yielding plants
Authors: Yadav Alpa , Batra Divya , Kaushik Prashant , Mohanta Tapan K. 
Published: April 2023

AbstractDue to their role in nutrient transmission, arbuscular mycorrhizal fungi (AMF) are widespread plant root symbionts. They may improve plant production by altering plant community structure and function. Therefore, a study was conducted in the state of Haryana to analyze the distribution pattern, diversity, and association of different AMF species with oil‐yielding plants. The results of the study revealed the percentage of root colonization, sporulation, and diversity of fungal species associated with the selected 30 oil‐yielding plants. The percentage root colonization ranged from 0% to 100%, the highest in Helianthus annuus (100.00 ± 0.00) and Zea mays (100.00 ± 0.00) and the least in Citrus aurantium (11.87 ± 1.43). At the same time, there was no root colonization in the Brassicaceae family. The number of AMF spores present in 50 g of soil samples varied from 17.41 ± 5.28 to 497.2 ± 8.38, with maximum spore population in Glycine max (497.2 ± 8.38) and minimum in Brassica napus (17.41 ± 5.28). Besides, the presence of several species of different genera of AMF was reported in all the studied oil‐yielding plants, that is, 60 AMF belonging to six genera viz. Acaulospora, Entrophospora, Glomus, Gigaspora, Sclerocystis, and Scutellospora were observed. Overall, this study will promote AMF usage in oil‐yielding plants.
Surgical site infection after pelvic bone and soft tissue sarcoma resection: Risk factors, microbiology, and impact of extended postoperative antibiotic prophylaxis
Authors: Bensaid Samuel , Contejean Adrien , Morand Philippe , Enser Maya , Eyrolle Luc , Charlier Caroline , Kernéis Solen , Anract Philippe , Biau David , Canouï Etienne ,   Other Members of the CRIOACochin Group
Published: April 2023

AbstractBackgroundPelvic bone and/or soft tissue sarcoma removal surgeries are associated with a high rate of surgical site infection (SSI). The recommended antibiotic prophylaxis (ABP) duration is 24−48 h. We aimed to assess the impact of extended ABP (5 days) on the SSI rate and describe the microbiology of SSI in bone and/or soft tissue pelvic sarcomas.MethodsWe retrospectively included all consecutive patients who underwent pelvic bone and/or soft tissue sarcoma removal surgery between January 2010 and June 2020.ResultsWe analyzed 146 patients with pelvic bone (45, 31%) or soft tissue (101, 69%). Sixty patients (41%) developed SSI. SSI occurred in 13/28 (46.4%) in the extended ABP group versus 47/118 (39.8%) in the standard group (p = 0.53). In multivariable analysis, risk factors for SSI were surgery duration (OR: 1.94 [1.41−2.92] per h), stay in postoperative ICU for more than 2 days (12.0 [2.8−61.3]), and shred or autologous skin flap (39.3 [5.8−409.5]). Extended ABP was not associated with SSI. SSI were mainly polymicrobial with Enterobacterales (57.4%) and Enterococcus (45%).Conclusions and DiscussionPelvic bone and/or soft tissue sarcoma removal surgery is highly prone to postoperative infection. Extending the ABP to 5 days does not reduce the level of SSI.
Eu3+‐Activated Single‐Band Ratiometric Nanothermometry by Lattice Negative Thermal Expansion
Authors: Pu Luyao , Li Panlai , Zhao Jiaoyin , Wang Yu , Guo Dongxu , Li Leipeng , Wang Zhijun , Suo Hao 
Published: April 2023

AbstractSingle‐band ratiometric (SBR) thermometry has recently emerged as a powerful alternative to its dual‐emission counterparts because it can avoid the large uncertainties related to the emission spectral overlap or light absorption/scattering by the medium. Herein, a novel SBR thermometric scheme in Sc2Mo3O12:Eu3+ nanosheet that depends on thermal enhancement of charge‐transfer state absorption between O2− and Eu3+ is reported. Mechanistic investigation reveals the vital role of the lattice negative thermal expansion (NTE) for thermally enhanced Eu3+ emissions according to the configuration coordinate model. In contrast, serious thermal quenching of Eu3+ emissions is detected under the excitation wavelength corresponding to ground state absorption. Such excitation wavelength‐dependent thermal behavior of luminescence enabled SBR thermometry with high sensitivity and resolution (Sr = 2.0% K−1, δT = 0.121 K at 363 K). Finally, the applicability of the proposed SBR model to real‐world sensing scenarios is demonstrated using the as‐fabricated flexible thin‐film, offering accurate and real‐time temperature detection at the local hotspot in the electronic component.
An Unconventional Route for Synthesis and Solid‐State Processing of Low‐Entangled Ultra‐High Molecular Weight Isotactic Polypropylene
Authors: Romano Dario , Marroquin‐Garcia Ramiro , Gupta Virendrakumar , Rastogi Sanjay 
Published: April 2023

AbstractIntroducing the reverse micelle formation during polymerization, and thus avoiding the catalyst support, aggregated single crystals of ultra‐high molecular weight isotactic polypropylene having spherical morphology are obtained. The ease in flowability of the spherical nascent morphology, having a low‐entangled state in the non‐crystalline region of the single crystals in the semi‐crystalline polymer, allows the sintering of the nascent polymer in the solid state without melting. Thus maintains a low‐entangled state, and facilitates the translation of macroscopic forces to macromolecular length scale, without melting, leading to the formation of uniaxially drawn objects having unprecedented properties that can be used in the development of one component, high‐performance, easy‐to‐recycle composites. Thus having the potential of replacing difficult‐to‐recycle hybrid composites.
Probe‐based mass spectrometry approaches for single‐cell and single‐organelle measurements
Authors: Castro Daniel C. , Chan‐Andersen Peter , Romanova Elena V. , Sweedler Jonathan V. 
Published: April 2023

AbstractExploring the chemical content of individual cells not only reveals underlying cell‐to‐cell chemical heterogeneity but is also a key component in understanding how cells combine to form emergent properties of cellular networks and tissues. Recent technological advances in many analytical techniques including mass spectrometry (MS) have improved instrumental limits of detection and laser/ion probe dimensions, allowing the analysis of micron and submicron sized areas. In the case of MS, these improvements combined with MS's broad analyte detection capabilities have enabled the rise of single‐cell and single‐organelle chemical characterization. As the chemical coverage and throughput of single‐cell measurements increase, more advanced statistical and data analysis methods have aided in data visualization and interpretation. This review focuses on secondary ion MS and matrix‐assisted laser desorption/ionization MS approaches for single‐cell and single‐organelle characterization, which is followed by advances in mass spectral data visualization and analysis.
Cerebellar Syndrome Induced by Hypomagnesemia: A Treatable Cause of Ataxia Not to be Missed. Report of Three Cases and a Review of the Literature
Authors: Olmedo‐Saura Gonzalo , Pérez‐Pérez Jesús , Xuclà‐Ferrarons Tomás , Collet Roger , Martínez‐Viguera Ana , Kulisevsky Jaime 
Published: April 2023

ABSTRACTBackgroundMagnesium is an important intracellular cation involved in essential enzymatic reactions. It is necessary for neuronal function and its depletion can produce neurological symptoms such as cramps or seizures. Clinical consequences of its deficit in the cerebellum are less known and the diagnosis can be delayed because of the lack of awareness on this condition.CasesWe present three cases of cerebellar syndrome (CS) due to hypomagnesemia: A midline CS with myoclonus and ocular flutter and two cases of hemispheric CS, one of them entailed a Schmahmann's syndrome and the other suffered a seizure. MRI findings revealed cerebellar vasogenic edema and the symptoms improved after magnesium replacement in all cases.Literature ReviewWe reviewed 22 cases of CS due to hypomagnesemia, all with subacute onset (days to weeks). Encephalopathy and/or epileptic seizures were common. MRI findings were vasogenic edema involving the cerebellar hemispheres, the vermis, or the nodule. Up to 50% of patients presented hypocalcemia and/or hypokalemia. All the patients showed symptomatic improvement after magnesium replacement, but 50% showed significant sequelae, and 46% relapsed.ConclusionsHypomagnesaemia should always be considered in the differential diagnosis of CS as it has a potential treatment, and its early recognition can avoid recurrences and permanent cerebellar impairment.
The use of a skin paddle‐containing vein graft for arteriovenous looping in two‐stage free flap reconstruction of lower extremities
Authors: Ho Chun‐Yee , Roan Tyng‐Luen , Hsieh Chi‐Ying , Yu Yen‐Chen , Chen Yo‐Shen 
Published: April 2023

AbstractBackgroundArteriovenous (AV) looping prior to the lower extremity free flap reconstruction enables better venous drainage in flap circulation, leading to less flap complications and better survival. A two‐staged reconstruction including free tissue transfer after AV looping ensures a robust venous drainage of the flap. Arterialization of the AV loop results in less venous problems after the free flap reconstruction. However, major problems of this staged operation include AV loop kinking, heavy compression and loop exposure, leading to AV graft failure and interruption of surgical planning. The purpose of this article is to summarize probable flaws we noticed in conventional two‐stage lower limb reconstruction and overcome those problems using the skin paddle‐containing vein graft.MethodEight patients with lower limb defects underwent lower limb reconstruction surgery using this technique at our institute. The mean age was 52 years old. Of the eight patients, three of them have the defect due to infection. Three of them was due to trauma and three of them was due to full‐thickness burn. Five of the defects located at foot. The other three defects located at heel, knee, and pretibial region. All of them require AV looping because of unavailability of nearby recipient vessels. They all received a two‐stage operation, including the first stage AV looping with a skin paddle‐containing vein graft and the second stage definite free tissue transfer.ResultsThe mean defect size was 140 cm2 (72–225). The mean length of AV loops was 17.1 cm (8–25). The mean size of skin paddles for vein grafts was 19.4 cm2 (15–24). The mean size of free ALT flaps 154.4 cm2 (105–252). All eight patients experienced a smooth postoperative course with no major or minor complications. There were no graft thrombosis or graft rupture complications during the vascular maturation period. All eight AV loops survived during maturation. All eight patients progressed to the second stage surgery. Maturation time ranged from 5 to 7 days. Free ALT flap was employed during the second stage reconstruction. All flaps survived at the last follow‐up visit. There was no partial flap loss and complications. The mean follow‐up time was 12.25 months, ranging from 8 to 17 months.ConclusionThe skin paddle‐containing vein graft is an effective modification of the regular vein graft for AV looping procedure. The skin paddle prevents the underlying AV loop from compression, kinking and twisting during maturation. It also aids assessing patency of the AV loop and avoids the formation of adhesion between the AV loop and the surrounding tissue.
Low back pain in pregnant women: A necessary or an avoidable evil?
Authors: Ben Nessib Dorra , Armi Sawssem , Ferjani Hanène , Garsi Mariem , Ben Sassi Senda , Kerkeni Sarra , Ben Aissa Rania , Berraies Ghada , Kaffel Dhia , Mathlouthi Nabil , Maatallah Kaouther , Belghith Cyrine , Slimani Olfa , Hamdi Wafa 
Published: April 2023

AbstractObjectivesThe aim of this study was to determine the prevalence, the main features and the potential risk factors of LBP in pregnant women.MethodsThis cross‐sectional study included 173 pregnant women in the third trimester. Non‐inclusion criteria were severe mental disability and known previous history of musculoskeletal diseases. The participants were categorised into two groups: women with pregnancy‐related LBP and pain‐free women. Demographic, socio‐professional, clinical and obstetrical data were compared between the two groups using the appropriate statistical tests.ResultsThe mean age was 32.2 ± 5.4 years [17–45]. Among them, 108 (62.4%) reported one or more episodes of LBP during at least 7 days, mostly in the third semester (n = 71). The presence of LBP was significantly associated with history of LBP in previous pregnancies and jobs requiring prolonged standing. Active jobs and the presence of gestational complications were significantly more common in pain‐free women. In the multivariate analysis, LBP was independently predicted by the history of LBP in previous pregnancies and the absence of gestational complications.ConclusionsThe association of LBP with gestational complications as a protective factor has not yet been reported in previous studies. These complications are a common cause of hospitalisation, which represents a period of relative rest during pregnancy. Our results revealed that history of LBP in previous pregnancies, sedentary lifestyle prior to pregnancy and prolonged standing are the main risk factors of LBP. In contrast, rest and avoidance of physical overstrain during pregnancy may be protective factors.
Pelvic floor investigations for anal incontinence: Are they useful to predict outcomes from conservative treatment?
Authors: Cuinas Karina , Ferrari Linda , Igbedioh Carlene , Solanki Deepa , Williams Andrew , Schizas Alexis , Hainsworth Alison 
Published: April 2023

AbstractAimsThere are several options for treating anal incontinence (AI), with limited success rate in long‐term follow‐up. Patients' selection is important to avoid unnecessary investigations and therapies. The aim of this review is to assess the utility of pelvic floor investigations to predict success from conservative treatment in AI.MethodsBaseline demographics, severity scores, and pelvic floor investigations of 490 patients with AI symptoms were retrospectively reviewed. Patient‐reported outcomes were used to define success of conservative treatment.ResultsBivariate analysis showed that gender, St Mark's incontinence score, Bowel continence and quality of life domains of International Consultation on Incontinence Modular Questionnaire–Bowel symptoms score, Bristol stool chart, anal squeeze pressure, enterocoele, leak of contrast at rest, and dyssynergia in defecography were associated with patient's outcomes from conservative treatment (p < 0.05). Multivariate analysis showed that only the Bowel continence score was an independent predictor of patient's success with treatment.ConclusionsPelvic floor investigations are of limited value to predict success of conservative treatment and they should be reserved for patients who fail noninvasive management and might require surgical intervention.
Is designed data collection still relevant in the big data era?
Authors: Anderson‐Cook Christine M. , Lu Lu 
Published: April 2023

AbstractThe excitement and buzz around big data (BD) has been so prevalent that it seems BD has become thought of as a panacea for solving all challenges. But there are in fact limitations to what BD can do, which has led to missed opportunities and potentially suboptimal problem‐solving and decision‐making. Hence the answer to the question posed in the title is an emphatic ‘Yes!’ The intentional strategic use of designed data collection (DDC) represents an opportunity to elevate how studies involving BD are planned, executed, and interpreted to deliver more satisfying solutions. In this paper, we outline eleven opportunities for integrating DDC strategies, including statistical design of experiments (DoE) and sampling techniques, with BD at all stages of the study. There are opportunities for building background understanding of the problem and improving the quality of the data via strategic planning before acquiring BD. In addition, incorporating DDC into the collection and processing of the actual BD, and strengthening conclusions or augmenting with supplementary designed data after BD also represent key opportunities to increase the impact of BD in the era of Industry 4.0 and Quality 4.0.
Online error rate control for platform trials
Authors: Robertson David S. , Wason James M. S. , König Franz , Posch Martin , Jaki Thomas 
Published: April 2023

Platform trials evaluate multiple experimental treatments under a single master protocol, where new treatment arms are added to the trial over time. Given the multiple treatment comparisons, there is the potential for inflation of the overall type I error rate, which is complicated by the fact that the hypotheses are tested at different times and are not necessarily pre‐specified. Online error rate control methodology provides a possible solution to the problem of multiplicity for platform trials where a relatively large number of hypotheses are expected to be tested over time. In the online multiple hypothesis testing framework, hypotheses are tested one‐by‐one over time, where at each time‐step an analyst decides whether to reject the current null hypothesis without knowledge of future tests but based solely on past decisions. Methodology has recently been developed for online control of the false discovery rate as well as the familywise error rate (FWER). In this article, we describe how to apply online error rate control to the platform trial setting, present extensive simulation results, and give some recommendations for the use of this new methodology in practice. We show that the algorithms for online error rate control can have a substantially lower FWER than uncorrected testing, while still achieving noticeable gains in power when compared with the use of a Bonferroni correction. We also illustrate how online error rate control would have impacted a currently ongoing platform trial.
‚Materialität‘ als Begriff und Kategorie der Korrelation von Recht und Literatur
Authors: Stiening Gideon 
Published: April 2023

ZusammenfassungDie Frage, in welchem Verhältnis Recht und Literatur zueinander stehen bzw. in welchen Formen sie zueinander in Beziehung gesetzt werden können oder gar müssen, lässt sich auf unterschiedliche Weise beantworten. Eine der grundlegenden Korrelationen besteht zweifellos darin, dass sich das Recht die Literatur und die Literatur das Recht jeweils zu ihrem Gegenstand machen. Dieses ebenso spezifische wie grundlegende Korrelationsverhältnis konstituiert Prozesse gegenseitiger Vergegenständlichung, die man zunächst mit dem beide übergreifenden Begriff der ‚Materialität‘ fassen kann, die die höchst unterschiedlichen Verobjektivierungen, nämlich die rechtliche Vergegenständlichung der Literatur und die literarische Vergegenständlichung des Rechts, kategorial zu fassen sucht. Da die Verrechtlichung der Literatur und die Literarisierung des Rechts zwei grundsätzlich unterschiedene Vorgänge sind, haben deren Gemeinsamkeiten im Rahmen der Formierung eines allgemeinen Begriffs der ‚Materialität‘ vor allem formalen Charakter.