bluuebunny/crossref_metadata_2025_split huggingface dataset:
Nicotinic acid protects germinal vesicle oocyte meiosis in mice and humans against toxicity of benzo(a)pyrene during maturation
Authors: Gao Min , Li Dungao , Zhan Shaoquan , Qiu Yanling , Chen Bohong , Cao Tianqi , Huang Jun jiu , Chen Zhiyun 
Published: May 2023

AbstractBackgroundApproximately 5 nM -7 nM internal exposure concentration of BaP was detected in women who mainstream smoke or suffering assisted reproductive failure. In this study, we evaluated the effects of benzo(a)pyrene (BaP) on mouse and human germinal vesicle (GV) oocyte maturation in 5 nM BaP. Then, we tested whether nicotinic acid (NA) could restore GV meiotic failure caused by the BaP or not during in vitro maturation (IVM)?MethodsClinically discarded GV oocytes from young women (aged < 35 years) undergoing intracytoplasmic sperm injection (ICSI) / in vitro fertilization (IVF) and GV oocytes from 6–8 weeks old female CD1 mice were used. Oocyte quality was estimated by GV oocyte maturation, morphological observation, and mitochondrial functions. The subcellular structures were further evaluated using immunostaining, fluorescent intensity quantification or western blot to analyze spindle organization, chromosome arrangement, actin polymerization, mitochondrial distribution, DNA damage and the Sirt1 protein level during mouse oocyte meiotic.ResultsWe found 5 nM/50 nM BaP exposure significantly reduced first polar body extrusion during mouse GV oocytes maturation. Sirt1 protein expression decreased after BaP treatment in mouse oocytes. Moreover, BaP exposure disorganized spindle and chromosome arrangement, disrupted cortical actin cap, impaired mitochondrial redistribution, and caused DNA damage in IVM metaphase II (MII) mouse oocytes. Importantly, NA supplementation (15µM) increased Sirt1 expression and significantly rescued most of the abnormal effects. We then explored the effect of 5 nM BaP on human GV oocytes, a concentration close to that in human ovarian follicular fluid, and found that BaP caused GV meiotic failure by increasing mitochondrial membrane potential and markedly elevating reactive oxygen species (ROS) levels. Finally, we showed that 15 µM NA supplementation partially rescued human GV oocytes from the toxicity of 5 nM BaP during IVM.ConclusionsCollectively, our study indicated that internal exposure concentrations of BaP could seriously disrupt GV oocyte IVM and caused GV meiotic defects in both mouse and human. NA partially protected GV oocyte meiosis against toxicity of BaP during IVM.
RACK1 mediates NLRP3 inflammasome activation during Pasteurella multocida infection
Authors: Ran Jinrong , Yin Hang , Xu Yating , Wang Yu , Li Gang , Wu Xingping , Peng Lianci , Peng Yuanyi , Fang Rendong 
Published: May 2023

Abstract
Pasteurella multocida is gram-negative bacteria that causes serious diseases in a wide range of animal species. Inflammasome as an intracellular multimolecular protein complex plays a critical role in host defense against microbial infection. Our previous study showed that bovine Pasteurella multocida type A (PmCQ2) infection induces NLRP3 inflammasome activation. However, the exact mechanism of PmCQ2-induced NLRP3 inflammasome activation is less studied. Here, we show that NLRP3 inflammasome activation is positive regulated by a scaffold protein called receptor for activated C kinase 1 (RACK1). This study showed that RACK1 expression was downregulated by PmCQ2 infection in mice primary peritoneal macrophages and mice tissues while overexpression of RACK1 rescued PmCQ2-induced cell death, indicating the positive role of RACK1 in the host. Next, RACK1 knockdown by si-RNA significantly attenuated PmCQ2-induced NLRP3 inflammasome activation with the reduction of protein expression of interleukin (IL)-1β, pro-IL-1β, caspase-1 and NLRP3 as well as the formation of ASC specks while RACK1 overexpression by pcDNA3.1-RACK1 plasmid transfection significantly promoted PmCQ2-induced NLRP3 inflammasome activation, indicating RACK1 is essential for NLRP3 inflammasome activation. Furthermore, RACK1 knockdown decreased PmCQ2-induced NF-κB activation but RACK1 overexpression increased its activation. In addition, results of immunofluorescence staining and immunoprecipitation showed that RACK1 colocalized with NLRP3 and NEK7 and interacted with them. However, blockage of potassium efflux significantly attenuated RACK1-NLRP3-NEK7 interaction. Our study demonstrated that RACK1 plays an important role in promoting NLRP3 inflammasome activation by regulating NF-κB and promoting the NLRP3 inflammasome assembly.
KDM2B regulates hippocampal morphogenesis by transcriptionally silencing Wnt signaling in neural progenitors
Authors: Zhou Yan , Zhang Bo , Zhao Chen , Shen Wenchen , Li Wei , Zheng Yue , Kong Xiangfei , Wang Junbao , Wu Xudong , Zeng Tao , Liu Ying 
Published: May 2023

Abstract
The hippocampus plays major roles in learning and memory, and its formation requires precise coordination of patterning, cell proliferation, differentiation, and migration. Here we removed the chromatin-association capability of KDM2B in the progenitors of developing dorsal telencephalon (Kdm2b∆CxxC) to discover that Kdm2b∆CxxC hippocampus, particularly the dentate gyrus, became drastically smaller with disorganized cellular components and structure. Kdm2b∆CxxC mice displayed prominent defects in spatial memory, motor learning and fear conditioning, resembling patients with KDM2B mutations. The migration and differentiation of neural progenitor cells was greatly impeded in the developing Kdm2b∆CxxC hippocampus. Mechanism studies revealed that Wnt signaling genes in developing Kdm2b∆CxxC hippocampi were de-repressed due to reduced enrichment of repressive histone marks by polycomb repressive complexes. Activating the Wnt signaling disturbed hippocampal neurogenesis, recapitulating the effect of KDM2B loss. Together, we unveiled a previously unappreciated gene repressive program mediated by KDM2B that controls progressive fate specifications and cell migration, hence morphogenesis of hippocampus.
Big Data Analytics in the Healthcare Sector: Opportunities and Challenges in Developing Countries. A Literature Review
Authors: Muhunzi David , Kitambala Lucy , Mashauri Harold 
Published: May 2023

Abstract
Background: The availability of vast amounts of healthcare data, along with the advancements in big data analytics techniques, has led to an unprecedented opportunity for healthcare organizations to improve patient outcomes and reduce healthcare costs. Despite the ongoing digitization of healthcare sector in developing countries, the full adoption of big data analytics in healthcare and the effective realization of its potential benefits still need to be reached. Exploring opportunities and challenges encountered in implementing data analytics in healthcare in developing countries is essential for designing implementation strategies and interventions.
Objective:Exploring opportunities and challenges towards integrating big data analytics technologies in the healthcare industry in developing countries.
Methodology: A literature search was conducted in PubMed, Science Direct and Google Scholar with the keywords "big data analytics", "big data analytics healthcare", "big data analytics healthcare developing countries", "healthcare data analytics", and "healthcare data analytics developing countries". Articles with predetermined keywords and written in English were included in the review.
Results:Big data analytics finds its application in medical images analysis and imaging informatics, population health management, clinical informatics and bioinformatics, as decision support algorithms even in developing countries. The major challenges to the clinical adoption of big data analytics in healthcare in developing countries include the need for more evidence for its practical benefits, competing priorities, the lack of trained personnel for analyzing big healthcare data and the lack of substantial financial investment required.
Conclusion:Integrating big data analytics in developing countries’ healthcare industry can potentially open new avenues for enhancing healthcare delivery. Despite the benefits big data analytics can have in the healthcare industry of developing countries, highlighted strategies should be implemented to address several challenges encountered towards using this technology.
Associations between Probiotic Exposure and yogurt consumption with Hepatic Steatosis: A nationwide cross-sectional study
Authors: Song Yancheng , Guo Wencong , Wang Junke , Liu Shuguang , Li Zhaopeng , Li Zhao , Guo Dong , Li Yu 
Published: May 2023

Abstract
Background Recent research showed that probiotics treatment may reduce insulin resistance, regulate lipid metabolism, raise liver enzyme levels, and ameliorate inflammation in individuals with non-alcoholic fatty liver disease (NAFLD). However, the association between probiotic use and hepatic steatosis in people has not been identified. The purpose of this study was to investigate the possible relationship between probiotic exposure and hepatic steatosis.Methods The cross-sectional research was conducted among adults with complete data on probiotic exposure and hepatic steatosis in the 2011–2016 National Health and Nutrition Examination Survey (NHANES). Probiotic exposure was assessed using a dietary supplement questionnaire. Hepatic steatosis was evaluated with hepatic steatosis index (HSI). Weighted multivariable regression analysis, subgroup analysis, and interaction analysis were conducted to explore the independent relationship between probiotic exposure and hepatic steatosis. The restricted cubic spline (RCS) was used to analyze the curve association between yogurt and hepatic steatosis.Results A total of 7891 participants were included in the study represented 146.7 million non-institutionalized residents of the United States, of whom 4322 (54.77%) were diagnosed with hepatic steatosis. Multivariable logistic regression showed probiotic exposure had significantly inverse associations for hepatic steatosis (OR = 0.84, 95% CI: 0.72–0.97, P = 0.02) after adjusting for all covariates. Once more, the independent relationship between probiotic exposure and hepatic steatosis was verified by subgroup analysis and interaction analysis. The restricted cubic spline model demonstrated a robust J-shaped link between yogurt and hepatic steatosis, revealing a significant decrease in risk within the lower range of yogurt, which attained the lowest risk close to 0.4 cup.Conclusions These results suggested that probiotic exposure may have beneficial effects for hepatic steatosis, along with a J-shaped relationship between yogurt and hepatic steatosis. This study recommends that people take half a cup of yogurt daily. Further research is required to identify the mechanistic relationships between probiotics exposure and hepatic steatosis.
Impact of long-lasting insecticidal nets on resting and feeding behaviour of Anopheles fluviatilis and Anopheles culicifacies (Diptera: Culicidae), the vectors of malaria in East- Central India
Authors: Sahu S. S. , Gunasekaran K. , Shriram A. N. , Panigrahi D. K. , Baig Mohammed Mustafa , Krishnamoorthy N. , Mathivanan A. , Kumar B Vijaya , Rahi Manju , Kumar Ashwani 
Published: May 2023

Abstract
Background & Objectives: Currently, the malaria control strategies in Odisha to achieve the goal of malaria elimination rely on long-lasing insecticidal nets. Shifting of behaviour in anopheline vectors poses a significant challenge for elimination of malaria. This manuscript discusses the shift in the behaviour of two primary vectors of malaria; Anopheles fluviatilis James s. l. and Anopheles culicifacies Giles s. l. after universal coverage of LLINs in Odisha State during 2017.
Methods: Mosquito collections were made fortnightly from the selected villages of Koraput and Malkangiri districts of Odisha State from April 2019 to March 2021. The entomological parameters; density, parity, sibling species composition, infection and human blood index of two malaria vectors and epidemiological parameters such as malaria incidence were assessed.
Results: The PMD of An. fluviatilis was zero in human dwellings in both the districts and the average PMD in cattle sheds was 1.5 in Koraput and 0.01 in Malkangiri district. In Koraput district, the average PMD of An. culicifacies was zero in human dwellings and 7.0 in cattle sheds and the corresponding values in Malkangiri district was 2.2 and 10.1, respectively. The foot-hill and plain ecotypes had a significantly greater abundance of An. fluviatilis and An. culicifacies, respectively. While winter season was the favourable period for the abundance of An. fluviatilis, both summer and rainy seasons are favourable period for An. culicifacies. The HBI of An. fluviatilis and An. culicifacies was 0.01 and 0.007, respectively.
Discussion: A shift in the resting behaviour of An. fluviatilis from human dwellings to cattle sheds and feeding behaviour from anthropophagy to zoophagy, change in sibling species composition from species S to T and reduction of vector infection status to zero was observed in this study. None was found positive for malaria in the study villages.
Conclusion: The study highlighted the necessity of continuing the ongoing vector control tool, i.e. LLINs for effective control of malaria.
Colchicine-induced Jasminum sambac Polyploids Secured Altered Metabolic Profile with Unique Antifungal Compounds
Authors: S Vishnupandi , M Ganga , K Rajamani , R Kannan , S Manonmani , Ashraf Suhail , Boopathi Manikanda 
Published: May 2023

Abstract
Cercospora leaf spot is one of the critical foliar fungal diseases of jasmine, which causes considerable yield losses. There are no cultivars or germplasm accessions that are resistant to this infection and hence fungicide usage is the mainstay of management of this disease, which poses a hazard to the environment. Polyploidization is one of the key breeding methods in jasmine as newly obtained polyploids may have increased resistance/tolerance to diseases owing to altered metabolite production. In the present study, seventeen putative polyploids were obtained from Jasminum sambac Cv. Ramanathapuram Gundumalli by treating with the polyploidizing agent, colchicine. Among them, two mutant plants (generated from the wild parent following treatment with 0.25% colchicine) exhibited no symptoms for Cercospora leaf spot, whereas the control had the highest mean PDI (63.65%). Non-targeted metabolite profiling experiment indicated that couple of antifungal compounds (such as 1H-1,2,4-Triazole-1-ethanol, α-butyl-α-(2,4-dichlorophenyl) and Dasycarpidan-1-methanol, acetate (ester)) were present only in the two newly evolved mutants of J. sambac and absent in wild parent. Molecular docking results have shown that the compound Dasycarpidan-1-methanol, acetate (ester) binds effectively with two fungal proteins 7EUS and 7EUT, which are actively involved in biosynthesis of cercosporin (which is a photoactivated toxin that uses reactive oxygen species (ROS) in host cells and damage cell components such as membranes, proteins and lipids). Thus, this study enabled the development of a natural and eco-friendly method of evolving and characterizing novel lines in jasmine, which would have potential applications in flower industry.
On numerical equivalence of parallel generalized solutions of mixed boundary value problem of annulus
Authors: Lin Luobin , Chen Fuquan , Huang Xianhai 
Published: May 2023

Abstract
This paper provides two parallel generalized solutions on a fundamental mixed boundary value problem that a unit annulus is subjected to a partially fixed outer periphery and an arbitrary traction acting along the inner periphery by using the complex variable method. The mixed problem is transformed into two parallel Riemann-Hilbert problems with respective sets of different mathematically rigorous boundary conditions, which are solved by successive approximation method with the Lanczos filtering technique to obtain numerically equivalent stress and displacement results. Four typical numerical cases coded by FORTRAN are carried out and compared to the same cases performed on ABAQUS to validate these two parallel solutions. Based on the case results, the detailed reasons of numerically equivalence between these two parallel solutions are analytically elaborated and several insights of the application of the complex variable method are revealed.
Migrant Men’s Views and Experiences towards Female Genital Mutilation/Cutting (FGM/C) and its Prevention and Care in OECD Countries: A Qualitative Systematic Review Protocol
Authors: Dwira Mark Hayford 
Published: May 2023

Abstract
Objective This review aims to synthesize male migrants’ views and experiences about the provision of care services for survivors of FGM/C and related-prevention strategies in healthcare settings in OECD countries.Introduction: Concerns among survivors about FGM/C care access relate to the potential impact of newly enacted laws in the healthcare service delivery and whether their decisions to seek care are acceptable by their current or future male partners. Little is known of their male partners’ views.Inclusion criteria: The review will consider articles that included male migrants of all ages who have close relationships with survivors and live in OECD countries. In studies comprising (men and women), where perspectives of men are reported separately, they will be included. Non-OECD countries’ studies will be excluded.Methods The databases to be searched will include Ovid MEDLINE, EBSCOhost, CINAHL, ASSIA, Ovid Embase, PsycINFO, Cochrane library, JBI Databases and hand searching of relevant journals. Unpublished studies will be searched through OpenGray and websites of national and international organisations working on FGM/C, including the National Health Services and World Health Organisation. The search will identify English-language publications from January 2002 onwards. Titles and abstracts will be reviewed first, and then full texts will be screened by two independent reviewers against the inclusion criteria. Any disagreements will be resolved through discussion or a third reviewer. The result will be critically appraised for methodological quality. Data extraction results will be synthesised and evaluated for credibility and dependability.Systematic review registration number: PROSPERO: CRD42019155518
User experience of a family health history chatbot: A quantitative analysis
Authors: Allen Caitlin 
Published: May 2023

Abstract
Objective: Family health history (FHx) is an important tool in assessing one’s risk towards specific health conditions. However, user experience of FHx collection tools is rarely studied. ItRunsInMyFamily.com (ItRuns) was developed to assess FHx and hereditary cancer risk. This study reports a quantitative user experience analysis of ItRuns.
Methods: We conducted a public health campaign in November 2019 to promote FHx collection using ItRuns. We used software telemetry to quantify abandonment and time spent on ItRuns to identify user behaviors and potential areas of improvement.
Results: Of 11065 users who started the ItRuns assessment, 4305 (38.91%) reached the final step to receive recommendations about hereditary cancer risk. Highest abandonment rates were during Introduction (32.82%), Invite Friends (29.03%), and Family Cancer History (12.03%) subflows. Median time to complete the assessment was 636 seconds. Users spent the highest median time on Proband Cancer History (124.00 seconds) and Family Cancer History (119.00 seconds) subflows. Search list questions took the longest to complete (median 19.50 seconds), followed by free text email input (15.00 seconds).
Conclusion: Knowledge of objective user behaviors at a large scale and factors impacting optimal user experience will help enhance the ItRuns workflow and improve future FHx collection.
The efficiency of the combination of topical and biological therapy in the treatment of nails psoriasis: a prospective study
Authors: Ostretsova M. N. , Kasikhina E. I. , Zhukova O. V. , Al-Hawatmi A.A-H.M. 
Published: May 2023

Introduction. The severity of the psoriatic process is associated with the involvement of the scalp, genitals, fold zones and nails in the process. The selection of effective therapy for psoriasis of difficult localizations is an urgent task of modern dermatology.Aim. To evaluate the effectiveness of treatment of moderate psoriasis and nail psoriasis with an IL-17A inhibitor in combination with a combined betamethasone dipropionate + salicylic acid ointment.Material and methods. A 12-week prospective study included data from 60 patients who were under observation at the Moscow Scientific and Practical Center of Dermatovenereology and Cosmetology in 2020-2021. Group 1 included 30 patients who received only the biological drug IL-17 inhibitor. Group 2 included 30 patients who received an IL-17 inhibitor in combination with topical therapy with an ointment of 0.05% betamethasone dipropionate and 3% salicylic acid (Rederm). The ointment was applied twice a day in two courses of 3 weeks with a break of 6 weeks between courses. When comparing indicators between groups, Student’s t-test was used for normal samples and the Mann- Whitney test for non- Gaussian samples.Results. When comparing PASI and NAPSI in patients of group 2 who received an IL-17 inhibitor in combination with external therapy with an ointment of 0.05% betamethasone dipropionate and 3% salicylic acid 2 times a day for two courses of 3 weeks with a break of 6 weeks between courses, the index values statistically significantly decreased after 12 weeks of therapy (p = 0.003) compared to group 1 (p = 0.015).Conclusions. The study showed that patients who received an IL-17 inhibitor in combination with a combined betamethasone dipropionate + salicycylic acid ointment achieved a significantly greater effect compared to patients who received only biologics. In addition, the combination with Rederm ointment has shown greater efficacy in the treatment of psoriatic onychodystrophy, which is difficult to treat even with biologics.
Techno-economic analysis of a hybrid electrodialysis–batch reverse osmosis process for brackish water desalination
Authors: Ankoliya Dipak , Mudgal Anurag , Sinha Manish Kumar , Patel Vivek , Patel Jatin 
Published: May 2023

Abstract

Hybridization of electrodialysis (ED) and batch reverse osmosis (BRO) process is used to reduce the brine volume and water production cost. The ED process has the benefit of high water volume recovery in brackish water desalination, while reverse osmosis can produce pure water at a low production cost. Here, a simple hybrid process layout is preferred in which the ED process is kept in the reject stream of the BRO process and permeate from both ED and BRO is mixed. Recovery of the ED process is kept at 70% which can decide the blending ratio of ED and BRO permeates. The capital cost and operating cost of ED and BRO processes are used to calculate water production cost. The water production cost from the hybrid ED–BRO process is found to be 0.22 $ m−3 of freshwater when the feed concentration is 1,100 ppm. The cost increases from 0.20 to 0.34 $ m−3 with feed concentration from 1,000 to 2,000 ppm. In the cost, a major portion comes from the capital equipment in which the highest contributor is the membrane for both ED and BRO processes.