bluuebunny/crossref_metadata_2025_split huggingface dataset:
Clinical observation of Porcine Collagen Membrane + artificial Bovine Bone Granules Guided tissue regeneration combined with Autologous CGF in the treatment of severe periodontitis bone defect
Authors: Cheng Jue , Cheng Lin , Kong Ling-Wei , Qiu Wenfeng , Zhuang Runtao 
Published: March 2023

Objective: To investigate and analyze the clinical observation of porcine collagen membrane + artificial bovine bone granules guided tissue regeneration (GTR) combined with autologous concentration of growth factors (CGF) in the treatment of severe periodontitis bone defect.
Methods: A total of 94 patients with severe periodontitis bone defects admitted to Shanxi Bethune Hospital from January 2019 to January 2022 were included. They were divided into two groups by simple randomization method. Patients in the control group were treated with porcine collagen membrane + artificial bovine bone granules GTR, while those in the observation group were treated with autologous CGF on the basis of the control group. Before and after treatment, periodontal clinical indicators [sulcus bleeding index (SBI), gingival retreat index (GR), probing depth (PD), clinical attachment loss (CAL), alveolar bone height (AH)] and bone resorption markers [Osteoprotegerin (OPG), bone gla protein (BGP), Type-1 collagen N-terminal peptide (NTX)] were compared between the two groups, and the incidence of postoperative complications in the two groups was recorded.
Results: The total efficacy of observation group was significantly higher than that of control group (p<0.05). Three months after surgery, the observation group had lower levels of SBI, PD, CAL and NTX while higher levels of GR, AH, OPG and BGP than the control group (p<0.05). There was no significant difference in complication rate between the two groups (p>0.05).
Conclusion: Porcine collagen membrane + artificial bovine bone granules GTR combined with autologous CGF boasts various benefits in the treatment of severe periodontitis bone defects, such as improvement of clinical outcomes, amelioration of periodontal tissue and inhibition of bone resorption.
doi: https://doi.org/10.12669/pjms.39.3.6899
How to cite this: Cheng J, Cheng L, Kong LW, Qiu W, Zhuang R. Clinical observation of Porcine Collagen Membrane + artificial Bovine Bone Granules Guided tissue regeneration combined with Autologous CGF in the treatment of severe periodontitis bone defect. Pak J Med Sci. 2023;39(3):710-714. doi: https://doi.org/10.12669/pjms.39.3.6899
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
The The effect of azoospermia factor microdeletions on intracytoplasmic sperm injection results in azoospermia patients
Authors: Emirdar Volkan , Acet Ferruh 
Published: March 2023

Background & Objective: Y chromosome abnormalities are common in male patients with severe oligo-azoospermia. In studies with karyotype analysis and cytogenetic methods, the importance of the Y chromosome in spermatogenesis has been well understood. Deletions in the azoospermia factor (AZF) localized at the distal end of the Y chromosome adversely affect the spermatogenesis process. Our objective was to determine the frequency of AZF microdeletion in azoospermia patients who underwent microTESE.
Methods: In this retrospective cohort study, 806 azoospermic men attending the In Vitro Fertilization (IVF) Center for infertility treatment between 2010 and 2022 were included. AZF deletion screening was conducted in all patients included in the study. Azoospermic patients with and without Y microdeletion were matched with the female’s age, cause of infertility, number of oocytes retrieved and number of metaphase II (MII) oocytes produced and compared. The primary outcome was the live birth rate (LBR). Pregnancy rate (PR) and clinical pregnancy rates (CPR) were secondary outcomes.
Results: We detected Y microdeletion in 55 (6.82%) of 806 infertile azoospermic men and 35 of them included in the study. Although the required gonadotropin dose and the total number of retrieved oocytes were similar, clinical pregnancy rates and live birth rates were found to be significantly lower in the microdeletion patient group (21.6% vs. 43%, p<0.05; and 18.9% vs. 36%, p<0.05, respectively).
Conclusions: Poor sperm quality in AZF microdeletion patients complicates the selection of appropriate sperm for ICSI. Therefore, it leads to a decrease in embryonic development, fertilization and pregnancy results. In order to select the best sperm for the use in ICSI procedure in this patient population, intracytoplasmic morphologically selected sperm injection (IMSI) method can be preferred to improve the cycle outcomes.
doi: https://doi.org/10.12669/pjms.39.3.7003
How to cite this: Emirdar V, Acet F. The effect of azoospermia factor microdeletions on intracytoplasmic sperm injection results in azoospermia patients. Pak J Med Sci. 2023;39(3):672-676. doi: https://doi.org/10.12669/pjms.39.3.7003
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Comparison of single and double centrifugation methods for preparation of Platelet-Rich Plasma (PRP)
Authors: Saqlain Nazish , Mazher Naghmana , Fateen Tooba , Siddique Asma 
Published: March 2023

Objective: To compare the platelet count, platelet concentration/yield, residual Red blood cells (RBCs) and White blood cells (WBCs) counts in platelet-rich plasma (PRP) samples prepared from the single- and the double-centrifugation protocols.
Methods: It was a Cross-Sectional study, conducted at the Department of Hematology & Transfusion Medicine, The Children’s Hospital and UCHS, Lahore from October 2021 to January 2022 including 50 voluntary, healthy individuals of age 20-45 years of both genders, after taking informed consent. Complete blood count analysis of all participants was done initially by drawing 3ml blood in EDTA vial. From all the participants, 20 ml venous blood sample was taken in syringes containing tri-sodium citrate and then shifted to harvest tubes. Group-I comprised of PRP samples prepared by single- centrifugation method. While Group-II samples were prepared by Double-centrifugation method consisting of soft and hard spin. The platelet, RBC and WBC counts in prepared PRP samples were determined by using automated SYSMEX XP-100 hematology analyzer. Platelet yield or Platelet concentration (%) was calculated for samples using formula. The data analysis was done using SPSS version 23.
Results: The mean PRP platelet count in Group-I was 594.6±157.4×103/µl whereas in Group-II was 923.06 ± 127.58×103/µl. In Group-I, the mean platelet concentration/yield in PRP was 175.75 ± 55.08% while in Group-II, it was 276.78 ± 112.7%. Significant difference was observed between the platelet counts and platelet concentration/yields from the PRP samples of two Group-s (p < 0.01). Significant difference between the WBCs count was also observed (p < 0.01) with higher WBCs in Group-I PRP. Residual RBCs were almost same among two Group-s.
Conclusions: The double centrifugation protocol resulted in higher platelet quantity and yield with less contamination by red and white blood cells than did the single centrifugation protocol for PRP preparation. So, double centrifugation method is beneficial in preparation of autologous as well as allogenic PRP.
doi: https://doi.org/10.12669/pjms.39.3.7264
How to cite this: Saqlain N, Mazher N, Fateen T, Siddique A. Comparison of single and double centrifugation methods for preparation of Platelet-Rich Plasma (PRP). Pak J Med Sci. 2023;39(3):634-637. doi: https://doi.org/10.12669/pjms.39.3.7264
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Quantitative evaluation of cardiac magnetic resonance feature tracking (CMR-FT) derived strain in patients with ST-elevation myocardial infarction
Authors: Saeed Sahrai , Eriksen Erlend , Larsen Terje H. 
Published: March 2023

doi: https://doi.org/10.12669/pjms.39.3.7571
How to cite this: Saeed S, Eriksen E, Larsen TH. Quantitative evaluation of cardiac magnetic resonance feature tracking (CMR-FT) derived strain in patients with ST-elevation myocardial infarction. Pak J Med Sci. 2023;39(3):629-630. doi: https://doi.org/10.12669/pjms.39.3.7571
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
A proof of a trace formula by Richard Melrose
Authors: Colin de Verdière Yves 
Published: March 2023

Abstract
The goal of this article is to give a new proof of the wave trace formula proved by Richard Melrose in an impressive article. This trace formula is an extension of the Chazarain-Duistermaat-Guillemin trace formula (denoted as “CDG trace formula” in this article) to the case of a sub-Riemannian Laplacian on a 3D contact closed manifold. The proof uses a normal form constructed in previous papers, following the pioneering work of Melrose to reduce the case of the invariant Laplacian on the 3D-Heisenberg group. We need also the propagation of singularities results of the works of Ivrii, Lascar, and Melrose.
The IWH Forecasting Dashboard: From Forecasts to Evaluation and Comparison
Authors: Heinisch Katja , Behrens Christoph , Döpke Jörg , Foltas Alexander , Fritsche Ulrich , Köhler Tim , Müller Karsten , Puckelwald Johannes , Reichmayr Hannes 
Published: March 2023

Abstract
The paper describes the “Halle Institute for Economic Research (IWH) Forecasting Dashboard (ForDas)”. This tool aims at providing, on a non-commercial basis, historical and actual macroeconomic forecast data for the Germany economy to researchers and interested audiences. The database renders it possible to directly compare forecast quality across selected institutions and over time. It is partly based on data collected in the DFG-funded project “Macroeconomic forecasts in great crisis”.
World Heart Day: Clinical case to raise awareness on cardiovascular disease in women
Authors: Sun Marie Chan 
Published: March 2023

AbstractThe World Heart Federation highlights that cardiovascular disease (CVD) is the leading cause of death in women worldwide. Indeed, women have unique female-specific risk factors for stroke, which include pregnancy, history of preeclampsia/eclampsia or gestational diabetes, duration of reproductive life, oral contraceptive use, and postmenopausal hormone therapy. In light of the female-specific risk factors and/or predisposing conditions, there is need to sensitize health professionals and the public in general on CVD in women. This clinical case is being shared with the written consent of the patient so as to avoid misdiagnosis or underdiagnosis of CVD among women.
Dilute hydrochloric acid induced switching from antagonism to synergism in the binary mixed systems of cocamidopropyl betaine (CAPB) and sodium dodecyl benzene sulfonate (SDBS)
Authors: Abdel-Rahem Rami A. 
Published: March 2023

Abstract
A synergism is reported in the binary mixtures of cocamidopropyl betaine (CAPB) and sodium dodecyl benzene sulfonate (SDBS) at mole fractions of 0.25, 0.50, and 0.75, in dilute hydrochloric acid solutions (∼0.05 M) HCl, at 25 °C. Such synergism is confirmed by surface tension values, critical micelle concentration (CMC), and interaction parameter values according to Holland and Rubingh’s model and the satisfaction of Hua and Rosen conditions. Surface excess (Γ) at air/water interface and the minimum area occupied by surfactant molecule (A
min) also indicated a reduction in the A
min of mixed CAPB/SDBS compared to single surfactants that is due to the strong electrostatic interaction between surfactants heads. The composition of CAPB/SDBS mixed micelles, the interaction parameter (β) and the micellar activity coefficients (f
1 and f
2) were evaluated from the regular solution model for these binary amphoteric/anionic mixed systems. It is clear the CAPB surfactants are converted to the cationic form by charging in the presence of HCl, which enhances the interaction between the oppositely charged CAPB and SDBS heads and overcomes the unfavourable packing between the tails predicted in pure water. Such strong interaction was also reflected on the phase behavior of CAPB/SDBS binary mixed system. Accordingly, a switching from antagonism to synergism is reported for CAPB/SDBS binary mixed system in the presence of dilute hydrochloric acid.
How are ad scepticism levels of young consumers shaped? Testing with simultaneous quantile regression analysis
Authors: KIYMALIOĞLU Aslıhan 
Published: March 2023

Advertising is an important persuasion tool for marketing efforts shaping consumer attitudes and behavioural intentions. Among the factors determining consumer attitudes toward advertising is ad scepticism. Comprehending how consumer scepticism toward ads is formed is therefore considered contributory. With this purpose, the research hypothesising the effect of cynicism, self-esteem, and market mavens on ad scepticism was modelled, and 374 observations obtained from members of Generation Z were tested with simultaneous quantile regression analysis. The findings revealed the effect of each variable for different quantile conditions of ad scepticism. The originality of the research is twofold. The first one is using market mavens, which have not been subject to research regarding their effect on ad scepticism and contributing to ad scepticism nomologic framework by supporting the significant effect of this variable. And the second one provides a detailed picture of the effects of independent variables on different levels of ad scepticism.
An overview study on the TL and OSL dosimetry patent processes over time
Authors: Gasparian P. B. R. , Malthez A. L. M. C. , Mariano L. , Campos L. L. , Politano R. 
Published: March 2023

Since its discovery, ionizing radiation has been used in many different applications. Materials and methods have been developed to measure and quantify radiation doses. Thermoluminescence (TL) and Optically Stimulated Luminescence (OSL) are two techniques used for radiation dosimetry. Both TL and OSL are primarily applied in several areas, such as dating of ancient materials, equipment quality control and individual monitoring. One of the parameters to measure the knowledge and development of a technology is the number of patents related to the field. In this work, we established a methodology for patent search on the World Intellectual Property Organization (WIPO) database aiming to review the development of TL and OSL dosimetry over time. We concluded that along with the OSL technique development, the TL technique should continue to be explored in radiation dosimetry.
Phytochemical investigation and exploration of the central nervous system depressant activity of ethanolic extract of the flowers of Jasminum grandiflorum L. as folklore medicine in Swiss albino mice
Authors: Khan Irshad , Malpani Arati , Ali Nisar 
Published: March 2023

Background: In Indian traditional medicine, the flowers of Jasminum grandiflorum L. (Oleaceae) are claimed to possess powerful central nervous system (CNS) depressant activity. Despite these traditional claims, no in-depth scientific study has been performed on the CNS depressant activity of the flowers of J. grandiflorum. Therefore, the present study was aimed at evaluating the CNS depressant activity of an ethanolic extract of the flowers of J. grandiflorum in Swiss albino mice.
Methods: Acute oral toxicity tests were done at doses of 550, 1750, and 5000 mg/kg. The extract was also subjected to phytochemical tests and TLC tests. The CNS depressant activity of the EEJG was evaluated by various models, such as the forced swimming test, tail suspension test, thiopental sodium-induced sleeping time test, locomotor activity test, and muscle co-ordination test, at two different doses (500 and 1000 mg/kg) in Swiss albino mice. Diazepam (1 mg/kg) was used as a standard drug.
Results: In acute oral toxicity studies, the extract was found to be safe up to a dose level of 5000 mg/kg body weight. EEJG at both doses (500 and 1000 mg/kg) showed significantly (p<0.05, p<0.01) increase in immobility time in the forced swimming test and tail suspension test. In the thiopental-induced sleeping time test, EEJG at 1000 mg/kg showed a significant (p<0.05) effect on the onset of action time and also significantly (p<0.01) increase the duration of sleeping time. EEJG at 1000 mg/kg showed a significant (p<0.01) decrease in locomotor activity, and the EEJG at both doses (500 and 1000 mg/kg) showed a significantly (p<0.05, p<0.01) decreased in muscle co-ordination activity when compared to control group.
Conclusions: The present study confirms the significant CNS depressant activity of the ethanolic extract of the flowers of J. grandiflorum, which may be due to flavonoids and steroids present in the extract as phytoconstituents confirmed by TLC. This study supports the plant's traditional use as a CNS depressant.
Counsellors’ perceived health promoting behaviours for enhancing physical well-being of clients
Authors: Helen Efeyadu Uzoekwe , Grace Chinyere Nnadi , Obianuju Blessing Mokwelu , Edna Nkechi Ofojebe, , Taiwo Abiodun Ojo 
Published: March 2023

This study adopts a descriptive survey design to seek for information, opinions and attitudes of the respondents and it is carried out in Anambra state of Nigeria. A total number of 143 counsellors who are willing to participate in the study are selected through accidental sampling technique. The instrument ‘perceived counsellors’ health promoting behaviours for enhancing physical well-being of clients (PCSHPBSFEPWBOCS) developed by the researchers based on the information from the literature reviewed is used for data collections through direct approach distribution. This instrument is validated by three experts. Test re-test was used to establish the reliability of the instrument and the reliability coefficient of 0.76 was obtained. The data collected are analyzed through the use of statistical weighted mean and ranking. Any mean score that is below 2.50 is accepted while any one below a mean score of 2.50 is rejected. The study revealed the following health promoting behaviours: engaging in daily exercise, taking healthy diet, minimizing hazards in the home and workplace, practicing effective hygiene, consumption of limited alcohol and tobacco, having adequate sleep and rest, little intake of salt, sugar and processed foods, adequate hydration, sunlight and fresh air having relaxed mind and maintaining a healthy body weight. The researchers recommend that Government should provide funds in support of the counsellors to educating and training people on promoting healthy behaviours that can enhance their physical well-being. Also, counsellors should increase the awareness on the adoption of these health promoting behaviours through the use of media and public announcements.
Molecularly Imprinted Polymers Based on Konjac for Selective Caffeine Adsorption in Aqueous Solution
Authors: WATTANANON Saranya , NARAKAEW Samroeng , CHAİSENA Aphiruk 
Published: March 2023

A number of caffeine extraction methods have been developed, such as microwave assisted extraction and ultrasonic-assisted extraction. The disadvantages of these methods are low selectivity, inconvenience, and inefficiency. Among the existing technologies, molecularly imprinted polymers (MIPs) are one of the most efficient and economical methods for the removal of caffeine contaminants. In this study, the objective was to prepare MIPs for the removal of complicated samples. The obtained materials were used as a sorbent for the extraction of caffeine from coffee brewed in an espresso. The MIPs were prepared using konjac/acrylic acid as a functional monomer, N, N′-methylenebisacrylamide as a cross-linker, and caffeine as a template. The chemical structures of MIPs were characterized by Fourier transform infrared spectroscopy. MIPs exhibited a higher maximum adsorption capacity (87.72 mg/g). The equilibrium adsorption data fit well with the Langmuir adsorption isotherm models, which confirm the monolayer adsorption behaviour of caffeine molecules on the surfaces of the MIPs samples. According to the experimental results of the adsorption capacity of caffeine from aqueous solution, the MIPs showed a higher percentage removal of caffeine (75.66%). Our findings suggest that MIPs are useful adsorbents for the decaffeination of coffee brewed in an espresso.
Determinants of game-products repurchasing behaviour in virtual comunity
Authors: Mifthakhul Zein Dhani , Puspaningrum Astrid , Rofiq Ainur 
Published: March 2023

The gaming industry is rapidly evolving, as indicated by the increased market sales of virtual products. Genshin Impact has risen to prominence after receiving the best mobile game 2021 prize at a game awards event. In addition, Genshin Impact will be ranked third in the world mobile game revenue list in 2021. The existence of a community that supports the development of a game is the most important component that contributes to its success. This study aims to examine the effect of virtual communities on online repurchase intention with customer engagement as a mediating variable and achievement as a moderating variable. Respondents in this study were members of the Genshin Impact Indonesia community who had purchased virtual products more than once. This study collected 180 samples which were distributed through various social media platforms such as Facebook. The data in this study were analyzed using SEM-PLS through the SmartPLS. The results of this study indicate that all hypotheses are accepted except for the role of consumer engagement in mediating the effect of virtual communities on online repurchase intention. The community plays a role in providing means of interaction among members and giving information about the game. The results of this study suggest that game developers provide rewards to the community to encourage interest in playing and purchasing in-game items.