bluuebunny/crossref_metadata_2025_split huggingface dataset:
Overcoming apoptotic resistance afforded by Bcl-2 in lymphoid tumor cells: a critical role for dexamethasone
Authors: Bortner Carl D. , Oakley Robert H. , Cidlowski John A. 
Published: December 2022

AbstractBcl-2 is an anti-apoptotic protein that promotes cell survival and resistance to cell death. Predictably, Bcl-2 as well as other anti-apoptotic Bcl-2 family members have been found to be overexpressed in a variety of human cancers. Approaches to overcome apoptotic resistance afforded by Bcl-2 in cells include anti-sense oligonucleotides, drugs that inhibit Bcl-2 function, and BH3 mimics have not been universally effective; thus, the need to understand the underlying mechanism of this resistance is vital. Glucocorticoids are stress hormones that act through their cognate receptors to control the transcription of numerous target genes, and in turn regulate a diverse array of biological processes. Synthetic glucocorticoids, such as dexamethasone, are prescribed in many chemotherapy protocols for neoplasms of lymphoid origin based on their ability to inhibit lymphocyte proliferation and promote apoptosis. However, lymphoid cells expressing Bcl-2 are resistant to glucocorticoid-induced cell death. We observed both pro- and anti-apoptotic characteristics in lymphoid cells expressing Bcl-2 following glucocorticoid treatment. These cells exhibited a profound change in their intracellular ionic composition, but a limited apoptotic ion flux and the absence of cell death. Provocatively, mimicking the loss of intracellular potassium using a low dose of a microbial toxin that acts as a potassium ionophore in combination with dexamethasone overcame the resistance afforded by Bcl-2 and killed the cells. Extending our study using other potassium ionophores revealed that direct depolarization of the mitochondria membrane potential coupled with prior treatment with glucocorticoids is the key mechanism for activating the cell death program and bypassing the resistance afforded by Bcl-2 in lymphoid cells. Finally, we show that the duration of dexamethasone pre-treatment is critical for regulating distinct genes and signaling pathways that sensitize the cells to die.
Urban population structure and dispersal of an Australian mosquito (Aedes notoscriptus) involved in disease transmission
Authors: Paris Véronique , Rane Rahul V. , Mee Peter T. , Lynch Stacey E. , Hoffmann Ary A. , Schmidt Thomas L. 
Published: December 2022

AbstractDispersal is a critical parameter for successful pest control measures as it determines the rate of movement across target control areas and influences the risk of human exposure. We used a fine-scale spatial population genomic approach to investigate the dispersal ecology and population structure of Aedes notoscriptus, an important disease transmitting mosquito at the Mornington Peninsula, Australia. We sampled and reared Ae. notoscriptus eggs at two time points from 170 traps up to 5 km apart and generated genomic data from 240 individuals. We also produced a draft genome assembly from a laboratory colony established from mosquitoes sampled near the study area. We found low genetic structure (Fst) and high coancestry throughout the study region. Using genetic data to identify close kin dyads, we found that mosquitoes had moved distances of >1 km within a generation, which is further than previously recorded. A spatial autocorrelation analysis of genetic distances indicated genetic similarity at >1 km separation, a tenfold higher distance than for a comparable population of Ae. aegypti, from Cairns, Australia. These findings point to high mobility of Ae. notoscriptus, highlighting challenges of localised intervention strategies. Further sampling within the same area 6 and 12 months after initial sampling showed that egg-counts were relatively consistent across time, and that spatial variation in egg-counts covaried with spatial variation in Wright’s neighbourhood size (NS). As NS increases linearly with population density, egg-counts may be useful for estimating relative density in Ae. notoscriptus. The results highlight the importance of acquiring species-specific data when planning control measures.
Direct attenuation of Arabidopsis ERECTA signalling by a pair of U-box E3 ligases
Authors: Chen Liangliang , Cochran Alicia M. , Waite Jessica M. , Shirasu Ken , Bemis Shannon M. , Torii Keiko U. 
Published: December 2022

AbstractPlants sense a myriad of signals through cell-surface receptors to coordinate their development and environmental response. The Arabidopsis ERECTA receptor kinase regulates diverse developmental processes via perceiving multiple EPIDERMAL PATTERNING FACTOR (EPF)/EPF-LIKE peptide ligands. How the activated ERECTA protein is turned over is unknown. Here we identify two closely related plant U-box ubiquitin E3 ligases, PUB30 and PUB31, as key attenuators of ERECTA signalling for two developmental processes: inflorescence/pedicel growth and stomatal development. Loss-of-function pub30 pub31 mutant plants exhibit extreme inflorescence/pedicel elongation and reduced stomatal numbers owing to excessive ERECTA protein accumulation. Ligand activation of ERECTA leads to phosphorylation of PUB30/31 via BRI1-ASSOCIATED KINASE1 (BAK1), which acts as a coreceptor kinase and a scaffold to promote PUB30/31 to associate with and ubiquitinate ERECTA for eventual degradation. Our work highlights PUB30 and PUB31 as integral components of the ERECTA regulatory circuit that ensure optimal signalling outputs, thereby defining the role for PUB proteins in developmental signalling.
Nanofiber formation as a promising technology for preservation and easy storage of extracellular vesicles
Authors: Németh Krisztina , Kazsoki Adrienn , Visnovitz Tamás , Pinke Balázs , Mészáros László , Buzás Edit I. , Zelkó Romána 
Published: December 2022

AbstractExtracellular vesicles (EVs) are cell-derived, membrane-enclosed particles with the potential for a wide range of future therapeutic applications. However, EVs have almost always been administered by direct injection, likely hindering their efficacy because of rapid clearance from the injection site. The present study aimed to incorporate medium-sized extracellular vesicles (mEVs) into fast-dissolving electrospun polyvinylpyrrolidone-based nanofibers to explore the storage-dependent structure–activity relationship of the resulting nanofibrous formulations. Aqueous polyvinylpyrrolidone-based precursor solutions were selected for the electrospinning process. The presence of EVs in the electrospun samples was confirmed by transmission electron microscopy, flow cytometry, and confocal laser scanning microscope. The results indicate that the fibrous structure of the samples was preserved until the end of the 12-week storage period. Furthermore, regardless of the storage temperature (4 °C or room temperature), nanofibers and nanofiber-associated EVs were present throughout the experimental period. Incorporating EVs into a stable solid polymeric delivery base could preserve their stability; meanwhile, according to the characteristics of the polymer, their targeted and controlled release can be achieved.
Microstructural response and wear behaviour of Ti-6Al-4V impregnated with Ni/Al2O3 + TiO2 nanostructured coating using an electric arc
Authors: Cooke Kavian , Alhubaida Abdulrahman 
Published: December 2022

AbstractTitanium alloys are known for their excellent corrosion resistance; however, low surface hardness results in poor wear resistance, which limits its potential application. This study employs a novel two-step process to embed a hard Ni coating containing a mixture of nanosized particles (Al2O3 and TiO2) into the surface of the Ti-6Al-4V alloy using an electric arc produced during the inert tungsten gas welding process. The surface of the sample was evaluated using Vickers Microhardness, Scanning electron microscopy, Energy dispersive spectroscopy and pin-on-plate wear testing. Microstructural analysis showed that impregnating the titanium surface with Ni/(Al2O3 and TiO2) nanomaterials resulted in the formation of a hard martensitic structure to a depth of approximately 2 mm below the surface. The changes observed are driven by modification of the surface chemistry and the presence of nickel, causing grain size reduction, solid solution strengthening and dispersion strengthening of the treated layer by the nanoparticles. The hardness of the treated layer increased by more than 180% when 40 nm Al2O3 and 30 nm TiO2 particles were embedded into the surface. Similarly, the wear resistance of the treated surface improved by 100%.
JAG1 is associated with the prognosis and metastasis in breast cancer
Authors: Qiao Xiaojuan , Ma Buhuan , Sun Weiting , Zhang Ning , Liu Yang , Jia Lizhou , Liu Caixia 
Published: December 2022

AbstractJagged canonical Notch ligand 1 (JAG1) regulates the progression of many cancers by the Notch signaling pathway, but its role in breast cancer (BC) remains unclear. In this research, JAG1 protein expression in BC tissues was detected by immunohistochemistry. The association between JAG1 and clinical significance was analyzed. The effect of JAG1 on malignant behaviors of BC cells was demonstrated by in vitro experiments. JAG1 expression in BC tissues was higher than that in para-carcinoma tissues. High JAG1 expression was significantly linked to advanced lymph node metastasis, distant metastasis, and the TNM stage. JAG1 was an independent prognostic factor for BC patients. JAG1 knockdown inhibited the proliferation, motility, migration, and invasion of BC cells, and weakened adhesion and penetration abilities to the blood–brain barrier, whereas JAG1 overexpression had the opposite effects. JAG1 has the potential to be a prognostic marker and therapeutic target for BC patients.
Sleep quality among inpatients of Spanish public hospitals
Authors: Bellon Filip , Stremler Robyn , Rubinat-Arnaldo Esther , Padilla-Martínez Julia M. , Casado-Ramirez Elvira , Sánchez-Ortuño Montserrat , Gea-Sánchez Montserrat , Martin-Vaquero Yolanda , Moreno-Casbas Teresa , Abad-Corpa Eva 
Published: December 2022

AbstractSleep is a complex process and is needed both in health and illness. Deprivation of sleep is known to have multiple negative physiological effects on people’s bodies and minds. Despite the awareness of these harmful effects, previous studies have shown that sleep is poor among hospitalised patients. We utilized an observational design with 343 patients recruited from medical and surgical units in 12 hospitals located in nine Spanish regions. Sociodemographic and clinical characteristics of patients were collected. Sleep quality at admission and during hospitalisation was measured by the Pittsburgh Sleep Quality Index. Sleep quantity was self-reported by patients in hours and minutes. Mean PSQI score before and during hospitalisation were respectively 8.62 ± 4.49 and 11.31 ± 4.04. Also, inpatients slept about an hour less during their hospital stay. Lower educational level, sedative medication intake, and multi-morbidity was shown to be associated with poorer sleep quality during hospitalisation. A higher level of habitual physical activity has shown to correlate positively with sleep quality in hospital. Our study showed poor sleep quality and quantity of inpatients and a drastic deterioration of sleep in hospital versus at home. These results may be helpful in drawing attention to patients’ sleep in hospitals and encouraging interventions to improve sleep.
Intergenic splicing-stimulated transcriptional readthrough is suppressed by nonsense-mediated mRNA decay in Arabidopsis
Authors: Kurihara Yukio , Makita Yuko , Kawauchi Masaharu , Kageyama Ami , Kuriyama Tomoko , Matsui Minami 
Published: December 2022

AbstractRecent emerging evidence has shown that readthrough transcripts (RTs), including polycistronic mRNAs, are also transcribed in eukaryotes. However, the post-transcriptional regulation for these remains to be elucidated. Here, we identify 271 polycistronic RT-producing loci in Arabidopsis. Increased accumulation of RTs is detected in the nonsense-mediated mRNA decay (NMD)-deficient mutants compared with wild type, and the second open reading frames (ORFs) of bicistronic mRNAs are rarely translated in contrast to the first ORFs. Intergenic splicing (IS) events which occur between first and second genes are seen in 158 RTs. Splicing inhibition assays suggest that IS eliminates the chance of transcription termination at the polyadenylation sites of the first gene and promotes accumulation of RTs. These results indicate that RTs arise from genes whose transcription termination is relatively weak or attenuated by IS, but NMD selectively degrades them. Ultimately, this report presents a eukaryotic strategy for RNA metabolism.
ADAMTSL3 knock-out mice develop cardiac dysfunction and dilatation with increased TGFβ signalling after pressure overload
Authors: Rypdal Karoline B. , Olav Melleby A. , Robinson Emma L. , Li Jia , Palmero Sheryl , Seifert Deborah E. , Martin Daniel , Clark Catelyn , López Begoña , Andreassen Kristine , Dahl Christen P. , Sjaastad Ivar , Tønnessen Theis , Stokke Mathis K. , Louch William E. , González Arantxa , Heymans Stephane , Christensen Geir , Apte Suneel S. , Lunde Ida G. 
Published: December 2022

AbstractHeart failure is a major cause of morbidity and mortality worldwide, and can result from pressure overload, where cardiac remodelling is characterized by cardiomyocyte hypertrophy and death, fibrosis, and inflammation. In failing hearts, transforming growth factor (TGF)β drives cardiac fibroblast (CFB) to myofibroblast differentiation causing excessive extracellular matrix production and cardiac remodelling. New strategies to target pathological TGFβ signalling in heart failure are needed. Here we show that the secreted glycoprotein ADAMTSL3 regulates TGFβ in the heart. We found that Adamtsl3 knock-out mice develop exacerbated cardiac dysfunction and dilatation with increased mortality, and hearts show increased TGFβ activity and CFB activation after pressure overload by aortic banding. Further, ADAMTSL3 overexpression in cultured CFBs inhibits TGFβ signalling, myofibroblast differentiation and collagen synthesis, suggesting a cardioprotective role for ADAMTSL3 by regulating TGFβ activity and CFB phenotype. These results warrant future investigation of the potential beneficial effects of ADAMTSL3 in heart failure.
Impaired muscle stem cell function and abnormal myogenesis in acquired myopathies
Authors: Deprez Alyson , Orfi Zakaria , Rieger Lupann , Dumont Nicolas Alexandre 
Published: December 2022

Abstract
Skeletal muscle possesses a high plasticity and a remarkable regenerative capacity that relies mainly on muscle stem cells (MuSCs). Molecular and cellular components of the MuSC niche, such as immune cells, play key roles to coordinate MuSC function and to orchestrate muscle regeneration. An abnormal infiltration of immune cells and/or imbalance of pro- and anti-inflammatory cytokines could lead to MuSC dysfunctions that could have long lasting effects on muscle function. Different genetic variants were shown to cause muscular dystrophies that intrinsically compromise MuSC function and/or disturb their microenvironment leading to impaired muscle regeneration that contributes to disease progression. Alternatively, many acquired myopathies caused by comorbidities (e.g., cardiopulmonary or kidney diseases), chronic inflammation/infection, or side effects of different drugs can also perturb MuSC function and their microenvironment. The goal of this review is to comprehensively summarize the current knowledge on acquired myopathies and their impact on MuSC function. We further describe potential therapeutic strategies to restore MuSC regenerative capacity.
Predictive energy management strategy for battery energy storage considering battery degradation cost
Authors: Amini Mohammad , Nazari Mohammad Hassan , Hosseinian Seyed Hossein 
Published: December 2022

AbstractThis study proposes a novel predictive energy management strategy to integrate the battery energy storage (BES) degradation cost into the BES scheduling problem and address the uncertainty in the energy management problem. As the first step, the factors affecting the BES calendar aging and cycle aging are linearly modelled. Furthermore, a linear algorithm is provided to calculate the BES cycle aging due to the BES complete and incomplete cycles. Subsequently, a novel approach to estimating the BES degradation cost function according to the BES specifications and degradation process is presented. Finally, taking into account the BES degradation cost model, the proposed predictive energy management strategy framework is implemented on an integrated photovoltaic and BES system to evaluate the applicability and efficiency of the proposed scheme in integrating the BES degradation cost in the energy management problem. The numerical simulation results indicate that integrating the BES degradation cost into the energy management problem significantly affects the BES charge/discharge strategy. Moreover, comparing the proposed predictive energy management strategy with a simple one, it is verified that the provided approach could decrease the BES capacity fade and degradation cost by 5.06% and 4.67%, respectively, and increase the photovoltaic farm profit by 1.10%.
The widestk-set of disjoint paths problem
Authors: Ribeiro Marco Antônio , Carvalho Iago Augusto , Pereira Armando Honório 
Published: December 2022

Finding disjoint and widest paths are key problems in telecommunication networks. In this paper, we study the Widestk-set of Disjoint Paths Problem (WKDPP), anNP-Hard optimization problem that considers both aspects. Given a digraphG = (N,A), WKDPP consists of computingkarc-disjoint paths between two nodes such that the sum of its minimum capacity arcs is maximized. We present three mathematical formulations for WKDPP, a symmetry-breaking inequality set, and propose two heuristic algorithms. Computational experiments compare the proposed heuristics with another from the literature to show the effectiveness of the proposed methods.
Bewegungstherapie 2.0
Authors: Huber Gerhard 
Published: December 2022

ZusammenfassungFür die meisten chronischen Erkrankungen hat unter allen medizinischen
Interventionen die körperliche Aktivität das beste
Verhältnis von Risiko und Nutzen und wahrscheinlich auch das beste
Kosten-Nutzen-Verhältnis. Leider wird diese Option im Gesundheitssystem
noch viel zu wenig genutzt. Körperliche Aktivität ist
für die Gesundheit des Menschen systemrelevant; ist aber nur
unzureichend in das System der Gesundheitsversorgung integriert. Es ist deshalb
notwendig, die traditionellen Konzepte zu erweitern und neue Perspektiven
für die Bewegungstherapie zu eröffnen. Dabei geht es um die
Frage, wie und warum Bewegung und körperliche Aktivität wirken
und wie bewegungsbezogene Interventionen zu differenzieren sind. Neuere
Forschungen zeigen, dass für die körperliche Aktivität
z. B. auf der Ebene der Exerkine weitaus mehr Wirkmechanismen
für die Gesundheitseffekte verantwortlich sind als bisher angenommen.
Das erweiterte Spektrum der Wirkmechanismen kann u. a. die Grundlage
dafür bilden, die Konzepte für die Bewegungsförderung
und die Bewegungstherapie weiterzuentwickeln. Der Beitrag soll
Anstöße für die dazu notwendige Diskussion liefern, bei
der der Bewegungswissenschaft eine tragende Rolle zukommt.
Surgical Treatment of Sublingual Gland Ranulas
Authors: Gontarz Michał , Bargiel Jakub , Gąsiorowski Krzysztof , Marecik Tomasz , Szczurowski Paweł , Zapała Jan , Gałązka Krystyna , Wyszyńska-Pawelec Grażyna 
Published: December 2022

Abstract
Introduction Ranulas are divided into oral (OR) and plunging (PR) and comprise the most common pathology of the sublingual gland. This study presents a case series of patients operated due to OR and PR within different type of modalities in a 1-year period.
Objective The aim of this study is to determine the optimal surgical treatment of ranulas based on our results as well as in the literature review.
Methods The medical charts of 7 patients with sublingual gland ranulas treated in 2020 were reviewed.
Results The median age of the patients was 19. Three patients with OR were treated by marsupialization, micromarsupialization, and sublingual gland excision. Four patients with PR were operated via cervical approach in three cases and intraoral approach in one case. No recurrence was observed in 14 months of follow-up, on average.
Conclusion Micromarsupialization should be consider as the primary treatment for OR. In case of recurrent OR and primary or recurrent PR, the best results might be obtained by radical excision of the sublingual gland, which can be performed without resection of the ranula sac with the intraoral approach.
A Comprehensive Radiologic Review of Shoulder Girdle Trauma
Authors: Nasir Muhammad Umer , Alsugair Faisal , Sheikh Adnan , Ouellette Hugue , Munk Peter , Mallinson Paul 
Published: December 2022

AbstractRadiologic knowledge of different fracture patterns involving the shoulder girdle is an important tool to generate clinically relevant reports, identify concomitant injuries, guide management decisions, and predict and minimize complications, such as nonunion, osteoarthritis, osteonecrosis, and hardware failure. Complex unstable injuries like scapulothoracic dissociation can also occur because of shoulder girdle trauma. Management options may vary from conservative to surgical, depending on the fracture type and patient factors. Injuries around the shoulder girdle can involve the glenohumeral articulation, scapula, superior shoulder suspensory complex, acromioclavicular joints, and scapulothoracic articulation.
Anterior Shoulder Instability
Authors: Christopher Holly W. , Grainger Andrew J. 
Published: December 2022

AbstractThe anatomy of the glenohumeral joint prioritizes mobility at the expense of stability and thus predisposes it to injury through dislocation. This article discusses the anatomical basis for instability and how it translates into patterns of injury, focusing on anterior instability. We suggest an approach to imaging the unstable shoulder and examine the imaging features seen in the context of anterior instability, along with consideration of postoperative appearances.
The Molecular Genetics of Colorectal Cancer, Hereditary Colorectal Cancer Syndromes, and Early-Onset Colorectal Cancer
Authors: Vu Joceline V. , Sommovilla Joshua 
Published: December 2022

AbstractWhile the incidence of colorectal cancer is decreasing for adults older than 50 years, there is a rise in colorectal cancer among individuals younger than 50 (termed early-onset colorectal cancer). This increase is multifactorial and reflects differences in screening, changes in environmental factors, and other influences. In this article, we review the molecular and genetic basis of sporadic colorectal cancer as well as inherited colorectal cancer syndromes. We also summarize the epidemiology of early-onset colorectal cancer and considerations for the treatment of this population of patients.