bluuebunny/crossref_metadata_2025_split huggingface dataset:
Mapping the functional expression of auxiliary subunits of KCa1.1 in glioblastoma
Authors: Feher Adam , Pethő Zoltán , Szanto Tibor G. , Klekner Álmos , Tajti Gabor , Batta Gyula , Hortobágyi Tibor , Varga Zoltan , Schwab Albrecht , Panyi Gyorgy 
Published: December 2022

AbstractGlioblastoma (GBM) is the most aggressive glial tumor, where ion channels, including KCa1.1, are candidates for new therapeutic options. Since the auxiliary subunits linked to KCa1.1 in GBM are largely unknown we used electrophysiology combined with pharmacology and gene silencing to address the functional expression of KCa1.1/β subunits complexes in both primary tumor cells and in the glioblastoma cell line U-87 MG. The pattern of the sensitivity (activation/inhibition) of the whole-cell currents to paxilline, lithocholic acid, arachidonic acid, and iberiotoxin; the presence of inactivation of the whole-cell current along with the loss of the outward rectification upon exposure to the reducing agent DTT collectively argue that KCa1.1/β3 complex is expressed in U-87 MG. Similar results were found using human primary glioblastoma cells isolated from patient samples. Silencing the β3 subunit expression inhibited carbachol-induced Ca2+ transients in U-87 MG thereby indicating the role of the KCa1.1/β3 in the Ca2+ signaling of glioblastoma cells. Functional expression of the KCa1.1/β3 complex, on the other hand, lacks cell cycle dependence. We suggest that the KCa1.1/β3 complex may have diagnostic and therapeutic potential in glioblastoma in the future.
Evolution of self-organised division of labour driven by stigmergy in leaf-cutter ants
Authors: Di Pietro Viviana , Govoni Patrick , Chan Kin Ho , Oliveira Ricardo Caliari , Wenseleers Tom , van den Berg Pieter 
Published: December 2022

AbstractSocial insects owe their widespread success to their ability to efficiently coordinate behaviour to carry out complex tasks. Several leaf-cutter ant species employ an advanced type of division of labour known as task partitioning, where the task of retrieving leaves is distributed between workers that cut and drop and those that collect the fallen leaves. It is not entirely clear how such highly coordinated behaviour can evolve, as it would seem to require the simultaneous mutations of multiple traits during the same generation. Here, we use an agent-based simulation model to show how task partitioning in leaf-cutter ants can gradually evolve by exploiting stigmergy (indirect coordination through the environment) through gravity (leaves falling from the treetop on the ground forming a cache). Our simple model allows independent variation in two core behavioural dimensions: the tendency to drop leaves and the tendency to pick up dropped leaves. Task partitioning readily evolves even under these minimal assumptions through adaptation to an arboreal environment where traveling up and down the tree is costly. Additionally, we analyse ant movement dynamics to demonstrate how the ants achieve efficient task allocation through task switching and negative feedback control.
Scheduled simple production method of pseudopregnant female mice for embryo transfer using the luteinizing hormone-releasing hormone agonist
Authors: Sari Gema Puspa , Hilario Patrick Louis Lagman , Yuri Shunsuke , Honda Arata , Isotani Ayako 
Published: December 2022

AbstractThe use of mice as experimental animal models has been a practice since the development of genetically engineered mouse models (GEMMs) in the early 1980s. New technologies, including genome editing, have helped in the time- and cost-efficient generation of GEMMs. However, methods for preparing pseudopregnant females, essential for the generation of GEMMs, remain less advanced. This study proposes a new method to achieve simple production of pseudopregnant female mice using a luteinizing hormone-releasing hormone agonist (LHRHa). A 20 µg LHRHa/mouse was identified as the best dose for inducing estrus synchronization. However, the frequency of copulation was 40% on a single injection. With sequential injections of 20 µg LHRHa/mouse on Days-1 and -2, followed by pairing on Day-5, 74% of LHRHa-treated females copulated with male mice. Moreover, LHRHa treatment did not affect fetal and postnatal development. Eventually, successful generation of offspring via embryo transfer was attained using LHRHa-treated pseudopregnant females. LHRHa administration method is efficient in producing pseudopregnant female mice for the generation of GEMMs, and we expect that it will contribute towards advancing the clinical research.
Exploring strong and weak topological states on isostructural substitutions in TlBiSe$$_2$$
Authors: Phutela Ankita , Bhumla Preeti , Jain Manjari , Bhattacharya Saswata 
Published: December 2022

AbstractTopological Insulators (TIs) are unique materials where insulating bulk hosts linearly dispersing surface states protected by the Time-Reversal Symmetry. These states lead to dissipationless current flow, which makes this class of materials highly promising for spintronic applications. Here, we predict TIs by employing state-of-the-art first-principles based methodologies, viz., density functional theory and many-body perturbation theory (G$$_0$$

0

W$$_0$$

0

) combined with spin-orbit coupling effects. For this, we take a well-known 3D TI, TlBiSe$$_2$$

2

and perform complete substitution with suitable materials at different sites to check if the obtained isostructural materials exhibit topological properties. Subsequently, we scan these materials based on SOC-induced parity inversion at Time-Reversal Invariant Momenta. Later, to confirm the topological nature of selected materials, we plot their surface states along with calculation of Z$$_2$$

2

invariants. Our results show that GaBiSe$$_2$$

2

is a strong Topological Insulator, besides, we report six weak Topological Insulators, viz., PbBiSe$$_2$$

2

, SnBiSe$$_2$$

2

, SbBiSe$$_2$$

2

, Bi$$_2$$

2

Se$$_2$$

2

, TlSnSe$$_2$$

2

and PbSbSe$$_2$$

2

. We have further verified that all the reported TIs are dynamically stable, showing all real phonon modes of vibration.
Simultaneous spectrofluorimetic determination of remdesivir and simeprevir in human plasma
Authors: El Sharkasy Mona E. , Tolba Manar M. , Belal Fathalla , Walash Mohamed I. , Aboshabana Rasha 
Published: December 2022

AbstractAs new infectious mutations of SARS-CoV-2 emerged throughout the world, innovative therapies to counter the virus-altered drug sensitivities were urgently needed. Several antiviral options have been in clinical trials or in compassionate use for the treatment of SARS-CoV-2 infections in an attempt to minimize both clinical severity and viral shedding. Recent research indicated that simeprevir acts synergistically with remdesivir, allowing for a multiple-fold decrease in its effective dose when used at physiologically acceptable concentrations. The goal of this work is to develop a sensitive synchronous spectrofluorimetric approach to simultaneously quantify the two drugs in biological fluids. Using this method, remdesivir and simeprevir could be measured spectrofluorimetrically at 283 and 341 nm, respectively, without interference from each other using Δλ of 90 nm. The effect of various experimental parameters on the fluorescence intensity of the two drugs was extensively explored and optimized. For each of remdesivir and simeprevir, the method exhibited a linearity range of 0.10–1.10 μg/mL, with lower detection limits of 0.01 and 0.02 μg/mL and quantification limits of 0.03 and 0.05 μg/mL, respectively. The high sensitivity of the developed method permitted the simultaneous determination of both drugs in spiked plasma samples with % recoveries ranging from 95.0 to 103.25 with acceptable standard deviation values of 1.92 and 3.04 for remdesivir and simeprevir, respectively. The validation of the approach was approved by the International Council of Harmonization (ICH) guidelines.
Direct detection of circulating donor-derived extracellular vesicles in kidney transplant recipients
Authors: Woud Wouter W. , Hesselink Dennis A. , Hoogduijn Martin J. , Baan Carla C. , Boer Karin 
Published: December 2022

AbstractExtracellular vesicles (EVs) are tissue-specific particles containing valuable diagnostic information. However, single EV analysis in blood is challenging due to their physical properties, the molecular complexity of plasma, and a lack of robust data interpretation methods. We assess the applicability of our recently-developed calibrated Imaging Flow Cytometry (IFCM)-based methodology to detect/characterize circulating tissue-specific EV subsets in the clinical setting of kidney transplantation. Platelet-poor plasma was generated from 36 HLA-A3 mismatched donor (HLA-A3 +) and kidney transplant recipients (KTRs; HLA-A3-). Samples taken before transplantation, 3 days, 7 days, and 6 months after transplantation as well as before ‘for-cause’ kidney transplant biopsies were stained with anti-CD9 (plasma EV-marker) and anti-HLA-A3. Before transplantation, no significant differences in total CD9 + EV concentrations were detected between donor and KTR samples. Tissue-specific EVs were identified as CD9 + HLA-A3 + . Serial dilution experiments of HLA-A3 + in HLA-A3- PPP showed that single CD9 + HLA-A3 + EVs were detectable down to ~ 1% above the recipient ‘self-signal’. After transplantation, CD9 + HLA-A3 + EVs were detected above pre-transplantation concentrations in individuals with stable allograft function, but not in individuals with allograft dysfunction. These results demonstrate the applicability of our calibrated IFCM-based methodology in the direct detection of tissue-specific EV subsets in clinical samples. We believe that this EV methodology is applicable in a variety of clinical contexts.
Adaptive Doppler‐smoothed‐code bilateral kernel regression method for single‐frequency BeiDou receiver
Authors: Jiang Yi , Shao Han , Gao Heng 
Published: December 2022

AbstractCurrently, single‐frequency Global Navigation Satellite System (GNSS) receivers dominate maritime navigation units due to their simple structure and low cost but usually cannot meet the positioning requirements of Maritime Autonomous Surface Ship (MASS). Herein, a novel adaptive Doppler‐smoothed‐code Bilateral Kernel Regression (DBKR) method is proposed, which improves pseudorange accuracy in the range domain and then reconstructs observations in the position domain. In the range domain, the Doppler observable is utilised to smooth the pseudorange with an optimal window smoothing width for the BeiDou Navigation Satellite System (BDS) receiver to alleviate ionosphere delay. In the position domain, we elaborate a bilateral kernel regression model to further reduce the positioning drift. The on‐line regression process starts with mapping the observation space to Euclidean space and subsequently fusing all observations of the same epoch using the Gaussian Radial Basis Function (RBF). Finally, the experiments under static and dynamic scenarios are carried out, which verify the validity and efficiency of the proposed DBKR method.
Observing circumplanetary disks with METIS
Authors: Oberg N. , Kamp I. , Cazaux S. , Rab Ch. , Czoske O. 
Published: December 2022

Context. Gaining a full understanding of the planet and moon formation process calls for observations that probe the circumplanetary environment of accreting giant planets. The mid-infrared ELT imager and spectrograph (METIS) will provide a unique capability to detect warm-gas emission lines from circumplanetary disks.
Aims. We aim to demonstrate the capability of the METIS instrument on the Extremely Large Telescope (ELT) to detect circumplanetary disks (CPDs) with fundamental v = 1−0 transitions of 12CO from 4.5 to 5 μm.
Methods. We considered the case of the well-studied HD 100546 pre-transitional disk to inform our disk modeling approach. We used the radiation-thermochemical disk modeling code ProDiMo to produce synthetic spectral channel maps. The observational simulator SimMETIS was employed to produce realistic data products with the integral field spectroscopic (IFU) mode.
Results. The detectability of the CPD depends strongly on the level of external irradiation and the physical extent of the disk, favoring massive (~10 MJ) planets and spatially extended disks, with radii approaching the planetary Hill radius. The majority of 12CO line emission originates from the outer disk surface and, thus, the CO line profiles are centrally peaked. The planetary luminosity does not contribute significantly to exciting disk gas line emission. If CPDs are dust-depleted, the 12CO line emission is enhanced as external radiation can penetrate deeper into the line emitting region.
Conclusions. UV-bright star systems with pre-transitional disks are ideal candidates to search for CO-emitting CPDs with ELT/METIS. METIS will be able to detect a variety of circumplanetary disks via their fundamental 12CO ro-vibrational line emission in only 60 s of total detector integration time.
Hidden depths in the local Universe: The Stellar Stream Legacy Survey
Authors: Martínez-Delgado David , Cooper Andrew P. , Román Javier , Pillepich Annalisa , Erkal Denis , Pearson Sarah , Moustakas John , Laporte Chervin F. P. , Laine Seppo , Akhlaghi Mohammad , Lang Dustin , Makarov Dmitry , Borlaff Alejandro S. , Donatiello Giuseppe , Pearson William J. , Miró-Carretero Juan , Cuillandre Jean-Charles , Domínguez Helena , Roca-Fàbrega Santi , Frenk Carlos S. , Schmidt Judy , Gómez-Flechoso María A. , Guzman Rafael , Libeskind Noam I. , Dey Arjun , Weaver Benjamin A. , Schlegel David , Myers Adam D. , Valdes Frank G. 
Published: December 2022

Context.Mergers and tidal interactions between massive galaxies and their dwarf satellites are a fundamental prediction of the Lambda-cold dark matter cosmology. These events are thought to provide important observational diagnostics of non-linear structure formation. Stellar streams in the Milky Way and Andromeda are spectacular evidence for ongoing satellite disruption. However, constructing a statistically meaningful sample of tidal streams beyond the Local Group has proven a daunting observational challenge, and the full potential for deepening our understanding of galaxy assembly using stellar streams has yet to be realised.Aims.Here we introduce the Stellar Stream Legacy Survey, a systematic imaging survey of tidal features associated with dwarf galaxy accretion around a sample of ∼3100 nearby galaxies withinz ∼ 0.02, including about 940 Milky Way analogues.Methods.Our survey exploits public deep imaging data from the DESI Legacy Imaging Surveys, which reach surface brightness as faint as ∼29 mag arcsec−2in therband. As a proof of concept of our survey, we report the detection and broad-band photometry of 24 new stellar streams in the local Universe.Results.We discuss how these observations can yield new constraints on galaxy formation theory through comparison to mock observations from cosmological galaxy simulations. These tests will probe the present-day mass assembly rate of galaxies, the stellar populations and orbits of satellites, the growth of stellar halos, and the resilience of stellar disks to satellite bombardment.
Acceptance of Acute Kidney Injury Alert by Providers in Cardiac Surgery Intensive Care Unit
Authors: Nevin Connor , Shawwa Khaled , Pincavitch Jami , Neely Rebecca L. , Goodwin Donnie , McCarthy Paul , Mohamed Nada , Mullett Charles , Smith Gordon S. , Kellum John A. , Sakhuja Ankit 
Published: December 2022

Abstract
Background Acute kidney injury (AKI) is a common complication after cardiac surgery and is associated with worse outcomes. Its management relies on early diagnosis, and therefore, electronic alerts have been used to alert clinicians for development of AKI. Electronic alerts are, however, associated with high rates of alert fatigue.
Objectives We designed this study to assess the acceptance of user-centered electronic AKI alert by clinicians.
Methods We developed a user-centered electronic AKI alert that alerted clinicians of development of AKI in a persistent yet noninterruptive fashion. As the goal of the alert was to alert toward new or worsening AKI, it disappeared 48 hours after being activated. We assessed the acceptance of the alert using surveys at 6 and 12 months after the alert went live.
Results At 6 months after their implementation, 38.9% providers reported that they would not have recognized AKI as early as they did without this alert. This number increased to 66.7% by 12 months of survey. Most providers also shared that they re-dosed or discontinued medications earlier, provided earlier management of volume status, avoided intravenous contrast use, and evaluated patients by using point-of-care ultrasounds more due to the alert. Overall, 83.3% respondents reported satisfaction with the electronic AKI alerts at 6 months and 94.4% at 12 months.
Conclusion This study showed high rates of acceptance of a user-centered electronic AKI alert over time by clinicians taking care of patients with AKI.
Sinonasal Outcomes Using Oral Corticosteroids in Patients with Chronic Rhinosinusitis with Nasal Polyps and Positive Sinonasal Cultures
Authors: Martinez-Paredes Jhon F. , Donaldson Angela M. , Marino Michael , Choby Garret , Olomu Osarenoma , Alfakir Razan , Stokken Janalee K. , O'Brien Erin , Lal Devyani 
Published: December 2022

Abstract
Introduction Patients with chronic rhinosinusitis with nasal polyps (CRSwNP) and positive sinonasal bacterial cultures may be recalcitrant to topical therapy alone due to the additional local inflammatory burden associated with bacterial infection/colonization.
Objective To evaluate sinonasal outcomes in CRSwNP patients with a positive perioperative bacterial culture, who were treated with postoperative intranasal corticosteroids (INCS) alone versus INCS in combination with a short-term course of oral corticosteroids (OCS).
Methods This is a retrospective chart review of CRSwNP patients. A total of 59 patients met inclusion criteria, including positive perioperative bacterial culture and treatment with INCS with or without concomitant use of OCS. Two cohorts were formed based on the chosen postoperative medical treatment; 32 patients underwent postoperative INCS alone, while 27 underwent INCS plus a ≤ 2-week course of OCS. The 22-item sinonasal outcome test (SNOT-22) scores and Lund-Kennedy scores (LKS) were assessed preoperatively, and at 2-week, 4-week, and 4 to 6 months after endoscopic sinus surgery (ESS).
Results There were no statistically significant differences in postoperative sinonasal symptoms or endoscopic scores between the cohorts treated with INCS plus OCS versus those prescribed INCS alone (p > 0.05). Our regression model failed to demonstrate a relationship between the use of OCS and better sinonasal outcomes at 2-week, 4-week, and 4 to 6 months after ESS (p > 0.05).
Conclusion Our study suggests that in a cohort of CRSwNP patients with recent bacterial infections, the postoperative use of combined OCS and INCS did not result in a statistical improvement of endoscopic and symptomatic outcomes over INCS irrigation alone. However, both treatment groups had a clinically significant improvement based on the Minimal Clinically Important Difference.
Acidic Microenvironment–Sensitive Core-Shell Microcubes: The Self-assembled and the Therapeutic Effects for Caries Prevention
Authors: Shih Tsai-Miao , Hsiao Jui-Fu , Shieh Dar-Bin , Tsai Guochuan Emil 
Published: December 2022

Abstract
Objectives The aim of this study was to develop a new material with integrated interface design that could achieve the purpose of environmental-sensing controlled release against cariogenic bacteria. Furthermore, this material can rebalance oral flora and serve as a preventive and reparative measure of dental caries.
Materials and Methods NaF@PAA@HA@polyelectrolytes@HA@PAA particles were synthesized using the method of two-solution phases precipitation followed by biocompatible polymers coating layer by layer. The structure of the particles was confirmed by transmission electron microscope. The fluoride release profile was measured by fluoride ion electrode. Antimicrobial activity against the cariogenic microorganisms was analyzed by scanning electron microscopy and energy dispersive spectrum. The efficacy experiments were conducted on tooth enamel slides to evaluated fluoride absorption and antibacterial activity of the prototype toothpaste containing microcube particles
Results The structure of NaF@PAA@HA@polyelectrolytes@HA@PAA particles showed a core surrounded by tooth-adhesion polymer layers in thin fin or filament structure. The loaded concentration of fluoride in the particles' core was 148,996 ± 28,484 ppm. NaF@PAA@HA@polyelectrolytes@HA@PAA particles showed selective inhibition of cariogenic microorganisms over probiotic strains and stronger fluoride adhesion on tooth enamel. A burst release (over 80%) of fluoride from the particle-containing toothpaste was observed under cariogenic acidic environment (pH < 5), while it remained extremely low under neutral environment. Compared with the best results of commercial toothpastes, our prototype toothpaste increased enamel fluoride uptake by 8-fold in normal enamel slides and by 11-fold in the slides with induced white spot lesions after either 1- or 7-day treatment. The prototype toothpaste also showed better inhibition of cariogenic microorganisms than the commercial brands. The coverage area of cariogenic bacteria under our toothpaste treatment was 73% on normal enamel slides compared with the commercial brands, while it was 69% in the induced white spot lesions.
Conclusions In our study, an intelligent toothpaste was developed that selectively inhibits cariogenic bacteria by microenvironment proton-triggered fluoride release. Such novel design would accomplish a favorable flora balance for optimal long-term oral health.
Iliocaval Reconstruction: Review of Technique, Challenges, and Outcomes
Authors: Kinariwala Dhara , Liles Amber , Williams David M. , Khaja Minhaj S. 
Published: December 2022

AbstractIliocaval thrombosis is a major source of morbidity for patients, with a range of clinical presentations, including recurrent lower extremity deep venous thrombosis and postthrombotic syndrome. Endovascular reconstruction of chronic iliocaval occlusion has been demonstrated to be a technically feasible procedure that provides long-lasting symptom relief in combination with antithrombotic therapy and close clinical monitoring. Herein, we describe the etiologies of iliocaval thrombosis, patient assessment, patient management prior to and after intervention, procedural techniques, and patient outcomes.
Varicoceles: Overview of Treatment from a Radiologic and Surgical Perspective
Authors: Neves da Silva Helio V. , Meller Robert L. , Ogundipe Eniola A. , Rochon Paul J. 
Published: December 2022

AbstractA testicular varicocele is the result of the expansion of the venous pampiniform plexus of the scrotum. Often painless, a significant number of patients experience orchialgia, swelling, testicular atrophy, and abnormalities in spermatic parameters. Treatment of symptomatic varicocele involves a radiologic or surgical intervention to obstruct the reflux of venous drainage. Testicular anatomy, diagnostic evaluation and imaging, options for surgical intervention, and a step-by-step description of retrograde embolization and antegrade scrotal sclerotherapy are discussed. Furthermore, included is an overview of postprocedural management and patient outcomes for radiological interventions, and the most up-to-date evidence on the efficacy of varicocele treatments as well as how they compare to each other.