bluuebunny/crossref_metadata_2025_split huggingface dataset:
Development of Web-Based Pascal Programming Multimedia at UPT SMK Negeri 2 Sidrap
Authors: Anjeliani Ayu , Lamanna Nurmayanti , Takdir Muhammad 
Published: May 2023

This study aims to develop web-based Multimedia Pascal Programming in the basic programming subject of TKJ class X UPT SMK Negeri 2 Sidrap. This type of research is research and development (Research and Development / R&D) with a 4D development model that has been developed by Thiagarajan, Semmel and Semmel (1974) which includes the stages of Define, Design, Develop, and Disseminate. Data collection uses observation instruments, interviews, and questionnaires which are then analyzed descriptively quantitatively. The results showed that Pascal Programming multimedia met the valid criteria based on the assessment of media and material validation with an average value of 85.5%. Thus, it can be concluded that multimedia Pascal Programming is declared very suitable for use in limited face-to-face learning UPT SMK Negeri 2 Sidrap.
Oxygen metabolism analysis of a single organoid for non-invasive discrimination of cancer subpopulations with different growth capabilities
Authors: Nashimoto Yuji , Shishido Shotaro , Onuma Kunishige , Ino Kosuke , Inoue Masahiro , Shiku Hitoshi 
Published: May 2023

Heterogeneous nature is a pivotal aspect of cancer, rendering treatment problematic and frequently resulting in recurrence. Therefore, advanced techniques for identifying subpopulations of a tumour in an intact state are essential to develop novel screening platforms that can reveal differences in treatment response among subpopulations. Herein, we conducted a non-invasive analysis of oxygen metabolism on multiple subpopulations of patient-derived organoids, examining its potential utility for non-destructive identification of subpopulations. We utilised scanning electrochemical microscopy (SECM) for non-invasive analysis of oxygen metabolism. As models of tumours with heterogeneous subpopulations, we used patient-derived cancer organoids with a distinct growth potential established using the cancer tissue-originated spheroid methodology. Scanning electrochemical microscopy measurements enabled the analysis of the oxygen consumption rate (OCR) for individual organoids as small as 100 µm in diameter and could detect the heterogeneity amongst studied subpopulations, which was not observed in conventional colorectal cancer cell lines. Furthermore, our oxygen metabolism analysis of pre-isolated subpopulations with a slow growth potential revealed that oxygen consumption rate may reflect differences in the growth rate of organoids. Although the proposed technique currently lacks single-cell level sensitivity, the variability of oxygen metabolism across tumour subpopulations is expected to serve as an important indicator for the discrimination of tumour subpopulations and construction of novel drug screening platforms in the future.
Helicobacter pylori infection selectively attenuates endothelial function in male mice via exosomes-mediated ROS production
Authors: Zhang Linfang , Xia Xiujuan , Wu Hao , Liu Xuanyou , Zhu Qiang , Wang Meifang , Hao Hong , Cui Yuqi , Li De-Pei , Chen Shi-You , Martinez-Lemus Luis A. , Hill Michael A. , Xu Canxia , Liu Zhenguo 
Published: May 2023

BackgroundSubstantial sex differences exist in atherosclerosis. Excessive reactive oxygen species (ROS) formation could lead to endothelial dysfunction which is critical to atherosclerosis development and progression. Helicobacter pylori (H. pylori) infection has been shown to attenuate endothelial function via exosomes-mediated ROS formation. We have demonstrated that H. pylori infection selectively increases atherosclerosis risk in males with unknown mechanism(s). The present study was to test the hypothesis that H. pylori infection impaired endothelial function selectively in male mice through exosome-mediated ROS formation.Methods and resultsAge-matched male and female C57BL/6 mice were infected with CagA+ H. pylori to investigate sex differences in H. pylori infection-induced endothelial dysfunction. H. pylori infection attenuated acetylcholine (ACh)-induced endothelium-dependent aortic relaxation without changing nitroglycerine-induced endothelium-independent relaxation in male but not female mice, associated with increased ROS formation in aorta compared with controls, which could be reversed by N-acetylcysteine treatment. Treatment of cultured mouse brain microvascular endothelial cells with exosomes from H. pylori infected male, not female, mice significantly increased intracellular ROS production and impaired endothelial function with decreased migration, tube formation, and proliferation, which could be prevented with N-acetylcysteine treatment.ConclusionsH. pylori infection selectively impairs endothelial function in male mice due to exosome-mediated ROS formation.
Using technology-based vocabulary instruction inside and outside of the classroom
Authors: Alahmadi Alaa , Albaqami Saad Eid , Foltz Anouschka 
Published: May 2023

IntroductionThe current pre-test post-test study explored the use of WhatsApp for vocabulary acquisition inside and outside the English as a Foreign Language classroom.MethodsNative Arabic undergraduate students learned English vocabulary items both inside and outside of the classroom using WhatsApp groups. Pre- and post-tests gauged participants, knowledge of the vocabulary items used in the intervention as well as control items. An additional questionnaire explored participants' satisfaction with using WhatsApp inside and outside of the classroom.ResultsWe found a small, but significant, vocabulary learning effect for using WhatsApp outside of the classroom, but no such effect for the use of WhatsApp inside the classroom. The questionnaire results additionally showed that students were highly satisfied with the use of WhatsApp to learn vocabulary, especially in terms of instructor availability. Finally, we found that students who were more motivated to use WhatsApp inside the classroom showed higher learning gains inside the classroom than those less motivated.DiscussionWe discuss the results with respect to the role of space and motivation for vocabulary acquisition using technologies such as WhatsApp.
Distributed stress fluidisation: Insights into the propagation mechanisms of the Abona volcanic debris avalanche (Tenerife) through a novel method for indurated deposit sedimentological analysis
Authors: Makris Symeon , Roverato Matteo , Dávila-Harris Pablo , Cole Paul , Manzella Irene 
Published: May 2023

Introduction: Volcanic debris avalanches mobilise large volumes and achieve long runouts with high destructive potential. However, the propagation processes that generate them are not currently explained by theoretical or numerical models, which are unable to represent deposit observations. Evaluation of the dynamics represented in deposits is therefore vital for constraining su ch models. The Abona volcanic debris avalanche deposit is located on the southern flank of the island of Tenerife, Spain. The deposit exhibits universal microfracturing and cataclasis. Fluidal features such as fluidal mixing of lithological units and diffuse boundaries, and mixed matrix are observed throughout the deposit.Methods: Field description including sedimentology and facies identification and the evaluation of their distribution have allowed the generation of a new conceptual model for the propagation dynamics of this volcanic debris avalanche, and potentially others with similar properties. The deposit is indurated making the detailed study of its sedimentology difficult, especially clast-size analysis. A novel method utilising structure from motion photogrammetry and photographic sampling was employed.Results: The universal cataclasis of the material and fluidal features suggest that the lack of a major competent material component allowed the mass to fragment and enabled fluidised granular flow behaviour. It is proposed that shear was periodically distributed throughout the body of the avalanche in chaotic temporary shear networks rearranging according to the instantaneous distribution of the mass. Stress and agitation were not temporally or spatially homogenous during propagation. This is also reflected in the unsystematic erosion of the substrate according to the variable basal shear accommodation.Discussion: It is proposed that lithological properties are potentially a determining factor for the propagation mechanisms, stress distribution, and consequently the evolution of a volcanic debris avalanche from the initial collapse to its emplacement. This study highlights the importance of dedicated field examinations of sedimentological, morphological, and structural features for providing constraints for models of volcanic debris avalanche dynamics and the factors dictating them. The novel methodology proposed has the potential of broadening the number of events that can be studied and enhancing the understanding of these complex and hazardous phenomena.
A provider survey of cystic fibrosis related diabetes screening and management practices at North American CF centers
Authors: Hicks Rebecca , Ode Katie Larson , Vigers Tim , Chan Christine L. 
Published: May 2023

BackgroundCystic Fibrosis Foundation (CFF) Guidelines recommend annual screening for cystic fibrosis related diabetes (CFRD) with an oral glucose tolerance test (OGTT). However, screening rates remain consistently low. We conducted surveys of 1) US CF center directors and 2) Endocrinologists affiliated with the CFF-sponsored EnVision program to characterize CFRD screening practices, describe provider perceived barriers to screening, and identify strategies for improving screening.MethodsThe surveys queried OGTT protocols, alternate screening strategies, and perceived barriers to screening. CF center characteristics and procedures for coordinating OGTTs were compared between centers achieving ≥50% versus &lt;50% OGTT completion. Endocrinologists received additional questions regarding OGTT interpretation and management.ResultsThe survey response rate was 18% (51/290) from CF Centers and 63% (25/40) from Endocrinologists. The majority (57%) of CF centers utilized 2 OGTT timepoints (0,120 min). The majority (72%) of Endocrinologists utilized 3 timepoints (0,60,120 min). Four percent of CF centers and 8% of Endocrinologists utilized other timepoints. Forty-nine percent of CF centers reported ≥50% OGTT completion in the past year. Completion of ≥50% OGTT was 5 times more likely when patient reminders were consistently provided (p = 0.017). Both CF Centers and Endocrinologists employed alternative screening strategies including HbA1c (64%, 92%), fasting plasma glucose (49%, 67%), continuous glucose monitoring (30%, 58%), and home fingerstick monitoring (55%, 50%).DiscussionOGTT is the gold standard screening method for CFRD, but completion rates remain suboptimal, practice variation exists, and many providers utilize alternate screening strategies. Systematic reminders may improve completion rates. Studies to improve our approach to CFRD screening are urgently needed.
Fecal microbiota transplantation for irritable bowel syndrome: a systematic review and meta-analysis of randomized controlled trials
Authors: Wang Mancai , Xie Xiaofeng , Zhao Songbo , Ma Xiaojuan , Wang Zheyuan , Zhang Youcheng 
Published: May 2023

ObjectiveWhether fecal microbiota transplantation (FMT) in patients with irritable bowel syndrome (IBS) is effective in improving outcomes remains controversial. We assessed the safety and efficacy of FMT for patients with IBS.MethodsIn this systematic review and meta-analysis, we searched PubMed, Embase, Web of Science, the Cochrane Library, the clinicaltrials.gov and International Clinical Trials Registry Platform (ICTRP) up to February 25, 2022, updated to March 28, 2023. Randomized controlled trials (RCTs) compared the stool and capsule FMT with placebo in patients with IBS were included. Two authors independently assessed study eligibility, extracted the data, and assessed risk of bias. We did meta-analysis with RevMan, and the Stata software was used for sensitivity analysis and meta-regression. The GRADE system was used to assess the quality of evidences. Mean difference (MD) or standardized Mean difference (SMD) with 95% CI for continuous data, and risk ratios (RR) with 95% CI for dichotomous data were used with random-effects models. The primary outcomes included the clinical response rate and IBS-SSS score. This study is registered with PROSPERO: CRD42022328377.ResultsNineteen reports from nine RCTs were included finally. Compared with the placebo, a single stool FMT could significantly decrease the IBS-SSS score at 1 month (MD=-65.75, 95%CI [-129.37, -2.13]), 3 months (MD=-102.11, 95% CI [-141.98, -62.24]), 6 months (MD=-84.38, 95%CI [-158.79, -9.97]), 24 months (MD=-110.41, 95%CI [-145.37, -75.46]), and 36 months (MD=-104.71, 95%CI [-137.78, -71.64]). It also could improve the clinical response rate at 3 months (RR=1.91, 95% [1.12, 3.25]), 24 months (RR=2.97, 95% [1.94, 4.54]), and 36 months (RR=2.48, 95% [1.65, 3.72]), and increase the IBS-QoL score at 3 months, 24 months, and 36 months. FMT did not increase the serious adverse event. The risk of bias was low, and the quality of evidence based on GRADE system was moderate in the stool FMT group. However, we did not find positive effect of capsule FMT on patients with IBS based on the current available data.ConclusionA single stool FMT is effective and safe for patients with IBS. However, some factors may affect the effectiveness of FMT, and the relationship between the gut microbiome and the effect of FMT for IBS is still unclear.Systematic review registrationhttps://www.crd.york.ac.uk/prospero/, identifier CRD42022328377.
Heart failure–related genes associated with oxidative stress and the immune landscape in lung cancer
Authors: Duan Ruoshu , Ye Kangli , Li Yangni , Sun Yujing , Zhu Jiahong , Ren Jingjing 
Published: May 2023

BackgroundLung cancer is a common comorbidity of heart failure (HF). The early identification of the risk factors for lung cancer in patients with HF is crucial to early diagnosis and prognosis. Furthermore, oxidative stress and immune responses are the two critical biological processes shared by HF and lung cancer. Therefore, our study aimed to select the core genes in HF and then investigate the potential mechanisms underlying HF and lung cancer, including oxidative stress and immune responses through the selected genes.MethodsDifferentially expressed genes (DEGs) were analyzed for HF using datasets extracted from the Gene Expression Omnibus database. Functional enrichment analysis was subsequently performed. Next, weighted gene co-expression network analysis was performed to select the core gene modules. Support vector machine models, the random forest method, and the least absolute shrinkage and selection operator (LASSO) algorithm were applied to construct a multigene signature. The diagnostic values of the signature genes were measured using receiver operating characteristic curves. Functional analysis of the signature genes and immune landscape was performed using single-sample gene set enrichment analysis. Finally, the oxidative stress–related genes in these signature genes were identified and validated in vitro in lung cancer cell lines.ResultsThe DEGs in the GSE57338 dataset were screened, and this dataset was then clustered into six modules using weighted gene co-expression network analysis; MEblue was significantly associated with HF (cor = −0.72, p &lt; 0.001). Signature genes including extracellular matrix protein 2 (ECM2), methyltransferase-like 7B (METTL7B), meiosis-specific nuclear structural 1 (MNS1), and secreted frizzled-related protein 4 (SFRP4) were selected using support vector machine models, the LASSO algorithm, and the random forest method. The respective areas under the curve of the receiver operating characteristic curves of ECM2, METTL7B, MNS1, and SFRP4 were 0.939, 0.854, 0.941, and 0.926, respectively. Single-sample gene set enrichment analysis revealed significant differences in the immune landscape of the patients with HF and healthy subjects. Functional analysis also suggested that these signature genes may be involved in oxidative stress. In particular, METTL7B was highly expressed in lung cancer cell lines. Meanwhile, the correlation between METTL7B and oxidative stress was further verified using flow cytometry.ConclusionWe identified that ECM2, METTL7B, MNS1, and SFRP4 exhibit remarkable diagnostic performance in patients with HF. Of note, METTL7B may be involved in the co-occurrence of HF and lung cancer by affecting the oxidative stress immune responses.
Effects of different dietary lipid sources (perilla, fish, and soybean oils) on growth, lipid metabolism, antioxidant, and immune status in Chinese giant salamander (Andrias davidianus)
Authors: Guo Yi-Hua , Ma Jun-Kang , Xu Hang-zhong , Li Wei-long , Liu Chang-Jiang , Lu Hua , Li Hong , Zhai Xu-liang , Xue Yang , Luo Li 
Published: May 2023

IntroductionPerilla oil is a natural vegetable oil rich in alpha-linolenic acid extracted from Perilla seeds. Fish oil is a commonly used animal fat containing a large amount of n-3 polyunsaturated fatty acids. Soybean oil is a commonly used vegetable oil rich in linoleic acid. This study aimed to determine whether perilla, fish, or soybean oil can be used as a lipid source in the diet of the Chinese giant salamander (Andrias davidianus).MethodsThree isonitrogenous and isolipidic diets were created by adding 3.0% each of soybean oil (SO), fish oil (FO), and perilla seed oil (PSO). Eight adolescent giant salamanders were fed in rectangular tanks for 98 days with a total of 96 giant salamanders (mean body weight = 75.20g) and were fed an experimental diet until satiation.Results and DiscussionThe viscerosomatic index and crude lipid content of muscle were found to be considerably lower and the muscle crude protein was substantially higher in the PSO group than in the other groups (P&lt;0.05). Linoleic acid levels were highest in SO, eicosapentaenoic acid and docosahexaenoic acid levels were highest in FO, and α-linolenic acid levels were highest in PSO in A. davidianus muscle tissue (P&lt;0.05). The results of study indicate that the PSO diet can significantly increase the total antioxidative capabilities in the liver and intestine, by elevating the activities of total superoxide dismutase, carnitine transferase-1, and acetyl-CoA carboxylase in the liver. Meanwhile, the immunoglobulin M and high-density lipoprotein cholesterol levels were higher in the blood (P&lt;0.05). Furthermore, it significantly reduced hepatic malondialdehyde, plasma endotoxin, D-lactic acid, and total cholesterol levels, and plasma alkaline phosphatase, diamine oxidase, aspartate transaminase, and alanine transaminase activities (P&lt;0.05). Therefore, perilla, fish, and soybean oils can be used as single lipid sources for A. davidianus with respect to growth performance. On the other hand, perilla oil can enhance crude protein content of muscle, increase Immunoglobulin M(IgM) content and Alkaline phosphatase(AKP) enzyme activity, promote lipid metabolism, and maintain the health of the liver and intestine in A. davidianus.
Adverse effects of hyperbaric oxygen therapy: a systematic review and meta-analysis
Authors: Zhang Yuyao , Zhou Yijun , Jia Yuanyuan , Wang Tiantian , Meng Dianhuai 
Published: May 2023

IntroductionHyperbaric oxygen therapy (HBOT) is one of the common clinical treatments, but adverse effects have hampered and limited the clinical application and promotion of hyperbaric oxygen therapy. A systematic review and meta-analysis of the adverse effects of hyperbaric oxygen therapy have conducted by our group to provide a theoretical basis for clinical treatment.MethodsThree electronic databases (PubMed, Web of Science, and The Cochrane Library) were comprehensively searched for randomized clinical trials (RCTs) from March 2012 to October 2022. Two reviewers independently screened titles and abstracts for eligibility and assessed the quality of the included studies. The meta-analysis was performed using RevMan 5.3.ResultsA total of 24 RCTs involving 1,497 participants were identified. ① The HBOT group reported more adverse effects (30.11% vs. 10.43%, p &lt; 0.05). ② The most frequent side effect of HBOT is ear discomfort (113 cases). ③ When the course of hyperbaric oxygen was &gt;10 sessions, the incidence of adverse effects was higher than that of the control group; when the course of HBOT was ≤10 sessions, the adverse effects caused by hyperbaric oxygen were comparatively lower. ④ When the chamber pressure is above 2.0 ATA, the incidence of adverse effects is higher than that of the control group. While the chamber pressure is lower than 2.0 ATA, HBOT is relatively safe compared with the previous one.ConclusionHyperbaric oxygen therapy (HBOT) is more likely to cause adverse reactions when the chamber pressure is above 2.0 ATA. More attention should be paid to the possible occurrence of related adverse effects if the treatment course is &gt;10 sessions.Systematic review registrationhttps://www.crd.york.ac.uk/PROSPERO/, identifier CRD42022316605.
Multiplex PCR-based next generation sequencing as a novel, targeted and accurate molecular approach for periprosthetic joint infection diagnosis
Authors: Huang Changyu , Huang Ying , Wang Ziwen , Lin Yiming , Li Yongbin , Chen Yang , Chen Xiaoqing , Zhang Chaofan , Li Wenbo , Zhang Wenming , Fang Xinyu , Huang Zida 
Published: May 2023

ObjectivesPeriprosthetic joint infection (PJI) diagnosis remains challenging, and the identification of the causative microorganism is, by far, the most important aspect. Here, we use multiple PCR-based targeted next-generation sequencing (tNGS) to detect pathogens in PJI. To explore 1. the ability of targeted next-generation sequencing (tNGS) to detect pathogens in PJI; 2. the consistency of tNGS, metagenomic NGS (mNGS), and culture results; and 3. the ability of tNGS to detect drug resistance genes in PJI.MethodsPJI was diagnosed according to the Musculoskeletal Infection Society (MSIS) criteria. The microorganisms were detected by culture, mNGS and tNGS to compare the diagnostic effectiveness of the three methods for PJI and to compare their consistency in detecting microorganisms. Drug resistance genes were detected using tNGS. The costs and turnaround times of mNGS and tNGS were compared.ResultsForty-three patients with PJI, 21 patients without PJI and 10 negative control cases were included. The culture, tNGS, and mNGS sensitivities for PJI diagnosis were 74.41%, 88.37%, and 93.02%, respectively, with no significant differences. The specificities were 90.48%, 95.24%, and 95.24%, respectively, with no significant differences. tNGS detected drug resistance genes in 37.5% of culture-positive PJIs. tNGS was superior to mNGS for turnaround time (14.5 h vs. 28 h) and cost ($150 vs. $260).ConclusionstNGS can effectively identify PJI pathogens and may provide drug resistance information, while tNGS is superior to mNGS regarding cost and turnaround time. A multidisciplinary, multi-technology based algorithm to diagnose PJI is appropriate.Highlights298 microorganisms and 86 drug resistance genes were included in the tNGS panel.Diagnostic efficacy of tNGS is not inferior to that of commonly used indicators.tNGS is superior to mNGS in cost and turnaround time.