bluuebunny/crossref_metadata_2025_split huggingface dataset:
Molecular and haematological characterisation of haemolytic anaemia associated with biallelic KLF1 mutations: a case series
Authors: Singha Kritsada , Teawtrakul Nattiya , Fucharoen Goonnapa , Fucharoen Supan 
Published: July 2023

AimsKrüppel-like factor 1 (KLF1) is an erythroid-specific transcription factor playing an important role in erythropoiesis and haemoglobin (Hb) switching. Biallelic KLF1 mutations can cause haemolytic anaemia with thalassaemia-like syndromes but are rarely reported. We explore the KLF1 mutations in Thai subjects with unexplainable haemolytic anaemia.MethodsThe study was done on 57 subjects presented with haemolytic anaemia and elevated Hb F without β-thalassaemia diseases. Hb analysis was performed using capillary electrophoresis. Analyses of α-thalassaemia, β-thalassaemia and KLF1 genes were performed using PCR-based methods and DNA sequencing.ResultsThirteen subjects with compound heterozygous for a known and five new genetic KLF1 interactions were identified, including KLF1:c.519_525dupCGGCGCC/c.892G>C with class 3/2 (n=8), and each subject with new genetic interaction, including KLF1:c.-154C>T;643C>T/c.983G>A with class 3/2, KLF1:c.-154C>T;643C>T/c.809C>G with class 3/2, KLF1:c892G>C/c.983G>A with class 2/2, KLF1:c.892G>C/c.1001C>G with class 2/2 and KLF1:c.1001C>G/c.1003G>A with class 2/2. Most of them had anaemia with Hb levels ranging from 45 to 110 g/L, hypochromic microcytosis, aniso-poikilocytosis, increased Hb F levels (17.9%–47.4%), small amounts of Hb Bart’s, regular blood transfusion, hyperbilirubinaemia, increased serum ferritin and nucleated red blood cell.ConclusionsBiallelic KLF1 mutations associated with anaemia may not be uncommon in Thailand. Characteristics of haemolytic anaemia, abnormal red cell morphology with nucleated red blood cells and elevated Hb F, and presenting small amounts of Hb Bart’s without thalassaemia diseases are useful markers to further investigation of the KLF1 gene.
Validation of three prediction models for thrombosis recurrence in antiphospholipid syndrome patients based on a prospective cohort
Authors: Zhao Yuan , Huang Can , Qi Wanting , Zhou Yangzhong , Zhao Jiuliang , Wang Qian , Tian Xinping , Li Mengtao , Zhao Yan , Zeng Xiaofeng 
Published: July 2023

ObjectivesTo validate the performance of the adjusted global antiphospholipid syndrome (APS) score (aGAPSS), Padua score and Caprini score to predict thrombosis recurrence in APS.MethodsConsecutive thrombotic-APS patients were included. aGAPSS, Padua and Caprini score at baseline were collected. Harrell c-index and calibration curve were used to validate the prediction models.Results362 patients were enrolled. The mean age was 36.30±13.88 years old, and 209 (57.7%) were female. Patients were followed up for a median of 2.32 years, with 32 (8.84%) venous and 21 (5.80%) arterial thrombosis. The 1-year, 3-year and 5-year thrombosis risks were 5.0%, 14.3% and 17.9%, respectively. The Harrell c-indexes of aGAPSS, Padua and Caprini score were 0.54 (95% CI 0.44 to 0.64), 0.54 (95% CI 0.46 to 0.62), and 0.50 (95%CI 0.42 to 0.58), respectively. Padua score had the best discrimination to predict venous thrombosis (Harrell c-index=0.61, 95% CI 0.53 to 0.69). aGAPSS had the best discrimination to predict arterial thrombosis (Harrell c-index=0.61, 95% CI 0.47 to 0.75). The calibrations for predicting thrombosis within 1, 3 and 5 years of the three models were suboptimal.ConclusionThe performance of aGAPSS, Padua and Caprini score to predict thrombosis recurrence in APS were suboptimal. Arterial and venous thrombosis recurrence predictors were different. New prediction models are required for venous and arterial thrombosis separately.
Effect of mixtures of legume species on ruminal fermentation, methane, and microbial nitrogen production in batch and continuous culture (RUSITEC) systems
Authors: Kelln B.M. , Penner G.B. , Acharya S.N. , McAllister T.A. , McKinnon J.J. , Saleem A.M. , Biligetu B. , Lardner H.A. 
Published: July 2023

The effect of cicer milkvetch ( Astragalus cicer L.) and sainfoin ( Onobrychis viciifolia Scop.) on ruminal fermentation, methane production, and microbial nitrogen synthesis was assessed in two experiments. Experiment 1 analyzed two legumes, cicer milkvetch and sainfoin at two stages (vegetative and late flower) incubated with alfalfa ( Medicago sativa) at five inclusion rates 0:100; 25:75, 50:50, 75:25, and 100:0 (as DM) in batch culture. Experiment 2 analyzed vegetative cicer milkvetch and alfalfa incubated in ratios of 25:75, 50:50, 75:25, and 100:0 (as DM) in continuous culture systems (RUSITEC). In batch culture, increased dry matter disappearance (DMD), and propionate percentage (%total), and reduced methane (mg·g−1 DMD) occurred with vegetative cicer milkvetch inclusion. In RUSITEC, DMD linearly increased ( P < 0.01), acetate:propionate ratio quadratically decreased, while ammonia (NH3-N) concentration ( P < 0.01) and butyrate percentage (%total) linearly decreased ( P < 0.05) with increasing inclusion of cicer milkvetch. No differences were observed for methane (CH4) production (mg·g−1 DMD), or short chain fatty acid (SCFA) production (mmol·day−1). Microbial nitrogen synthesis and efficiency of protein synthesis linearly increased ( P < 0.05) with increased inclusion of cicer milkvetch. Results suggest cicer milkvetch may result in synchronicity of energy and nitrogen during rumen fermentation, which could enhance cattle production.
Retracted article: Depth Edge and Structure Optimization-Based End-to-End Self-Supervised Stereo Matching
Authors: Yang Wenbang , Cheng Xianjing , Zhao Yong , Qian Ren , Li Jianhua 
Published: July 2023

The authors hereby formally submit a request to retract the manuscript titled "Depth Edge and Structure Optimization-Based End-to-End Self-Supervised Stereo Matching", which was submitted to IJPRAI on May 19, 2023, by Wenbang Yang and co-authors. The reasons for this retraction are as follows: 1. Errors or deficiencies were discovered in the data during the research process, which affect the scientific rigor and accuracy of the paper. 2. Unresolved academic ethical issues have been identified and require further verification and revision. 3. Upon verification, certain parts of the manuscript were found to overlap with previously published works, necessitating a thorough review and revision.
Trends and Outcomes of Cardiogenic Shock in Patients With End-Stage Renal Disease: Insights From USRDS Database
Authors: Dalia Tarun , Pothuru Suveenkrishna , Chan Wan-Chi , Mehta Harsh , Goyal Amandeep , Farhoud Hassan , Boda Ilham , Malhotra Anureet , Vidic Andrija , Rali Aniket S. , Hanff Thomas C. , Gupta Kamal , Fang James C. , Shah Zubair 
Published: July 2023

BACKGROUND:
There is a paucity of data regarding epidemiology, temporal trends, and outcomes of patients with cardiogenic shock (CS) and end-stage renal disease (chronic kidney disease stage V on hemodialysis).

METHODS:
This is a retrospective cohort study using the United States Renal Data System database from January 1, 2006 to December 31, 2019. We analyzed trends of CS, percutaneous mechanical support (intraaortic balloon pump, percutaneous ventricular assist device [Impella and Tandemheart], and extracorporeal membrane oxygenation) utilization, index mortality, 30-day mortality, and 1-year all-cause mortality in end-stage renal disease patients.

RESULTS:

A total of 43 825 end-stage renal disease patients were hospitalized with CS (median age, 67.8 years [IQR, 59.4–75.8] and 59.1% men). From 2006 to 2019, the incidence of CS increased from 275 to 578 per 100 000 patients (
P
trend
<0.001). The index mortality rate declined from 54.1% in 2006 to 40.8% in 2019 (
P
trend
=0.44), and the 1-year all-cause mortality decreased from 63% in 2006 to 61.8% in 2018 (
P
trend
=0.73), but neither trend was statistically significant. There was a significantly decreased utilization of intra-aortic balloon pumps from 17 832 to 7992 (
P
trend
<0.001), increased utilization of percutaneous ventricular assist device from 137 to 5201 (
P
trend
<0.001) and increase in extracorporeal membrane oxygenation use from 69 to 904 per 100 000 patients (
P
trend
<0.001). After adjusting for covariates, there was no significant difference in index mortality between CS patients requiring percutaneous mechanical support versus those not requiring percutaneous mechanical support (odds ratio, 0.97 [CI, 0.91–1.02];
P
=0.22). On multivariable regression analysis, older age, peripheral vascular disease, diabetes, and time on dialysis were independent predictors of higher index mortality.

CONCLUSIONS:
The incidence of CS in end-stage renal disease patients has doubled without significant change in the trend of index mortality despite the use of percutaneous mechanical support.
Community-Engaged Pedagogy in the Psychology Classroom: Shelter Dogs go to College
Authors: Flaisher-Grinberg Shlomit 
Published: July 2023

Background Community-engaged pedagogy has the potential to benefit both students and the community. Objective The purpose of this project was to design a new undergraduate psychology course according to recommended community-engaged practices and to explore learning outcomes associated with it. The Canine Learning and Behavior (CLB) course, taught by faculty and community co-educators, offered undergraduate psychology students the opportunity to train shelter dogs and write shelter-specific grant applications. Method Learning outcomes were assessed via course grades and self-evaluation questions for students enrolled in the CLB ( n = 21) and in a comparably challenging Sensation and Perception (SnP, n = 47) course, taught within the same semesters by the same faculty instructor. Results In comparison to participation in the SnP course, participation in the CLB course was associated with higher content comprehension, confidence in and familiarity with relevant skills (i.e., dog training and grant writing), as well as awareness of and sense of responsibility to the needs of the community. Conclusion The employment of community-engaged pedagogy can benefit various stakeholders, including students, the community, and in this specific case, shelter dogs. Teaching Implications This proof-of-concept study may encourage psychology educators to integrate community-engaged pedagogy into their curriculum.
Parent/Caregiver Experiences of a CAMHS ADHD Assessment Pathway: A Qualitative Service Evaluation
Authors: Kruger Emily 
Published: July 2023

Background: Attention deficit hyperactivity disorder (ADHD) is a neurodevelopmental condition characterized by impulsivity, inattention, and hyperactivity. Although the literature has explored the parental impact of living with a child diagnosed with ADHD, less is known about the ADHD assessment journey for children and the parental experiences of this. A recent service evaluation suggested that exploring parental perceptions of a child’s ADHD assessment pathway could be beneficial in understanding experiences, gathering ideas for future service development, and collecting service user feedback. Aims and Objective: The aims were to (a) explore the broad experiences of parents/caregivers accessing the ADHD assessment pathway for their child and (b) explore experiences of remote appointments during the assessment process in consideration of the COVID-19 pandemic. Methods: Twelve parents of children who had experienced an ADHD assessment within a CAMHS pathway participated in a telephone-based semi-structured interview. This involved gathering experiences of the CAMHS ADHD pathway from a parental perspective. The evaluation implemented a qualitative design. Qualitative data was analyzed through thematic analysis. Results: Results revealed seven main themes. Regarding general experiences of the pathway, parent/caregiver interview responses indicated a sense of relief, feeling understood, and the service being child-focused during the assessment. Difficulties were highlighted within the themes, such as long waiting lists impacting on the child and parent as well as lengthy assessments. In reference to COVID-19 and remote/telephone appointments, interview responses indicated some positives of having remote appointments, such as comfort and convenience. Thus, the strengths of face-to-face communication were highlighted.
Development and validation of automated electronic health record data reuse for a multidisciplinary quality dashboard
Authors: Ebbers Tom , Takes Robert P , Honings Jimmie , Smeele Ludi E , Kool Rudolf B , van den Broek Guido B 
Published: July 2023

Objective To describe the development and validation of automated electronic health record data reuse for a multidisciplinary quality dashboard. Materials and methods Comparative study analyzing a manually extracted and an automatically extracted dataset with 262 patients treated for HNC cancer in a tertiary oncology center in the Netherlands in 2020. The primary outcome measures were the percentage of agreement on data elements required for calculating quality indicators and the difference between indicators results calculated using manually collected and indicators that used automatically extracted data. Results The results of this study demonstrate high agreement between manual and automatically collected variables, reaching up to 99.0% agreement. However, some variables demonstrate lower levels of agreement, with one variable showing only a 20.0% agreement rate. The indicator results obtained through manual collection and automatic extraction show high agreement in most cases, with discrepancy rates ranging from 0.3% to 3.5%. One indicator is identified as a negative outlier, with a discrepancy rate of nearly 25%. Conclusions This study shows that it is possible to use routinely collected structured data to reliably measure the quality of care in real-time, which could render manual data collection for quality measurement obsolete. To achieve reliable data reuse, it is important that relevant data is recorded as structured data during the care process. Furthermore, the results also imply that data validation is conditional to development of a reliable dashboard.
Combined GLUT1 and OXPHOS inhibition eliminates acute myeloid leukemia cells by restraining their metabolic plasticity
Authors: Rodriguez-Zabala Maria , Ramakrishnan Ramprasad , Reinbach Katrin , Ghosh Somadri , Oburoglu Leal , Falqués-Costa Antoni , Bellamkonda Kishan , Ehinger Mats , Peña-Martínez Pablo , Puente-Moncada Noelia , Lilljebjörn Henrik , Cammenga Jörg , Pronk Cornelis Jan , Lazarevic Vladimir , Fioretos Thoas , Hagström-Andersson Anna K. , Woods Niels-Bjarne , Järås Marcus 
Published: July 2023

Abstract
Acute myeloid leukemia (AML) is initiated and propagated by leukemia stem cells (LSCs), a self-renewing population of leukemia cells responsible for therapy resistance. Hence, there is an urgent need to identify new therapeutic opportunities targeting LSCs. Here, we performed an in vivo CRISPR knockout screen to identify potential therapeutic targets by interrogating cell surface dependencies of LSCs. The facilitated glucose transporter type 1 (GLUT1) emerged as a critical in vivo metabolic dependency for LSCs in a murine MLL::AF9–driven model of AML. GLUT1 disruption by genetic ablation or pharmacological inhibition led to suppression of leukemia progression and improved survival of mice that received transplantation with LSCs. Metabolic profiling revealed that Glut1 inhibition suppressed glycolysis, decreased levels of tricarboxylic acid cycle intermediates and increased the levels of amino acids. This metabolic reprogramming was accompanied by an increase in autophagic activity and apoptosis. Moreover, Glut1 disruption caused transcriptional, morphological, and immunophenotypic changes, consistent with differentiation of AML cells. Notably, dual inhibition of GLUT1 and oxidative phosphorylation (OXPHOS) exhibited synergistic antileukemic effects in the majority of tested primary AML patient samples through restraining of their metabolic plasticity. In particular, RUNX1-mutated primary leukemia cells displayed striking sensitivity to the combination treatment compared with normal CD34+ bone marrow and cord blood cells. Collectively, our study reveals a GLUT1 dependency of murine LSCs in the bone marrow microenvironment and demonstrates that dual inhibition of GLUT1 and OXPHOS is a promising therapeutic approach for AML.
MRI features of idiopathic intracranial hypertension are not prognostic of visual and headache outcome
Authors: Bsteh Gabriel , Marik Wolfgang , Krajnc Nik , Macher Stefan , Mitsch Christoph , Pruckner Philip , Novak Klaus , Wöber Christian , Pemp Berthold 
Published: July 2023

Abstract
Background
In idiopathic intracranial hypertension (IIH), certain MRI features are promising diagnostic markers, but whether these have prognostic value is currently unknown.

Methods
We included patients from the Vienna-Idiopathic-Intracranial-Hypertension (VIIH) database with IIH according to Friedman criteria and cranial MRI performed at diagnosis. Presence of empty sella (ES), perioptic subarachnoid space distension (POSD) with or without optic nerve tortuosity (ONT), posterior globe flattening (PGF) and transverse sinus stenosis (TSS) was assessed and multivariable regression models regarding visual outcome (persistent visual impairment/visual worsening) and headache outcome (headache improvement/freedom of headache) were fitted.

Results
We included 84 IIH patients (88.1% female, mean age 33.5 years, median body mass index 33.7). At baseline, visual impairment was present in 70.2% and headache in 84.5% (54.8% chronic). Persistent visual impairment occurred in 58.3%, visual worsening in 13.1%, headache improvement was achieved in 83.8%, freedom of headache in 26.2%.
At least one MRI feature was found in 78.6% and 60.0% had ≥3 features with POSD most frequent (64.3%) followed by TSS (60.0%), ONT (46.4%), ES (44.0%) and PGF (23.8%).
In multivariable models, there was no association of any single MRI feature or their number with visual impairment, visual worsening, headache improvement or freedom.
Visual impairment at baseline predicted persistent visual impairment (odds ratio 6.3, p<0.001), but not visual worsening. Chronic headache at baseline was significantly associated with lower likelihood of headache freedom (odds ratio 0.48, p=0.013), but not with headache improvement.

Conclusions
MRI features of IIH are neither prognostic of visual nor headache outcome.
StackTTCA: a stacking ensemble learning-based framework for accurate and high-throughput identification of tumor T cell antigens
Authors: Charoenkwan Phasit , Schaduangrat Nalini , Shoombuatong Watshara 
Published: July 2023

Abstract
Background
The identification of tumor T cell antigens (TTCAs) is crucial for providing insights into their functional mechanisms and utilizing their potential in anticancer vaccines development. In this context, TTCAs are highly promising. Meanwhile, experimental technologies for discovering and characterizing new TTCAs are expensive and time-consuming. Although many machine learning (ML)-based models have been proposed for identifying new TTCAs, there is still a need to develop a robust model that can achieve higher rates of accuracy and precision.

Results
In this study, we propose a new stacking ensemble learning-based framework, termed StackTTCA, for accurate and large-scale identification of TTCAs. Firstly, we constructed 156 different baseline models by using 12 different feature encoding schemes and 13 popular ML algorithms. Secondly, these baseline models were trained and employed to create a new probabilistic feature vector. Finally, the optimal probabilistic feature vector was determined based the feature selection strategy and then used for the construction of our stacked model. Comparative benchmarking experiments indicated that StackTTCA clearly outperformed several ML classifiers and the existing methods in terms of the independent test, with an accuracy of 0.932 and Matthew's correlation coefficient of 0.866.

Conclusions
In summary, the proposed stacking ensemble learning-based framework of StackTTCA could help to precisely and rapidly identify true TTCAs for follow-up experimental verification. In addition, we developed an online web server (http://2pmlab.camt.cmu.ac.th/StackTTCA) to maximize user convenience for high-throughput screening of novel TTCAs.
Prevalence of tuberculosis and associated factors among presumptive TB refugees residing in refugee camps in Ethiopia
Authors: Meaza Abyot , Yenew Bazezew , Amare Miskir , Alemu Ayinalem , Hailu Michael , Gamtesa Dinka Fikadu , Kaba Mirgissa , Medhin Girmay , Ameni Gobena , Gumi Balako 
Published: July 2023

Abstract
Background
Tuberculosis (TB) causes significant morbidity and mortality in refugee populations. Although Ethiopia is the third largest refugee-hosting country in Africa, there is limited published data on the prevalence and associated factors of TB in refugees. The objective of this study was to estimate the prevalence of bacteriologically confirmed pulmonary TB (PTB) and explore associated factors in presumptive TB refugees residing in refugee camps in Ethiopia.

Methods
A facility-based cross-sectional study was conducted between February and August 2021 in refugee camps in Ethiopia. Data were collected consecutively from 610 presumptive TB refugees who attended for TB diagnosis in selected refugee camp clinics in Ethiopia. A pre-tested questionnaire was used to collect data, and sputum samples were collected from eligible study participants. The Xpert Mycobacterium tuberculosis (MTB)/Rifampicin (RIF) assay was performed on direct spot sputum samples, whereas morning sputum samples were processed and inoculated for bacteriological culture using Mycobacterium Growth Indicator Tube (MGIT) and Lowsteen Jensen (LJ) methods. The statistical software package (STATA version 14) was used for statistical analysis. A logistic regression model was used for the evaluation of the association between bacteriologically confirmed TB cases and the associated factors. Descriptive statistics were used for the expression of the results, and statistical significance was assumed at p < 0.05.

Results
Out of 610 study participants, more than half were female (54.9%), and the mean age was 37.9 years (SD, 16.64). The prevalence of bacteriologically confirmed PTB cases among refugees residing in refugee camps in Ethiopia was 13.3% (95% CI, 10.7–16.2%) using the Xpert MTB/RIF assay and/or culture. MTB was detected in 12.8% (95% CI, 10.2–15.7%) of the individuals using the Xpert MTB/RIF assay, while culture positivity was observed in 11.6% (95% CI, 9.2–14.5%). The multivariable logistic regression model showed South Sudan origins (adjusted odds ratio, AOR = 7.74; 95% CI, 3.05–19.64), age group, 19–38 years old (AOR = 5.66; 95% CI, 1.86–17.28), and male sex (AOR = 2.69; 95% CI, 1.58–4.56) were significantly associated with the bacteriologically confirmed TB among refugees residing in refugee camps in Ethiopia.

Conclusion
The prevalence of bacteriologically confirmed PTB among presumptive TB refugees residing in refugee camps in Ethiopia was high. The national TB program should strengthen TB prevention and control activities in the refugee camps of Ethiopia. Moreover, an active TB survey program should be implemented in refugee camps in Ethiopia.