bluuebunny/crossref_metadata_2025_split huggingface dataset:
Securin and cyclin B1-CDK1, but not SGO2, regulate separase activity during meiosis I in mouse oocytes
Authors: Wetherall Benjamin , Bulmer David , Sarginson Alexandra , Thomas Christopher , Madgwick Suzanne 
Published: January 2025

AbstractDuring meiosis I in oocytes, anaphase is triggered by deactivation of cyclin B1-CDK1 and activation of separase. Active separase plays an essential role in cleaving cohesin rings that hold homologous chromosomes together. Critically, separase must be inhibited until all chromosomes are aligned and the cell is prepared for anaphase I. Inhibition can be mediated through the binding of separase to either securin or cyclin B1-CDK1. The relative contribution of each inhibitory pathway varies depending on cell type. Recently, shugoshin-2 (SGO2) has also been shown to inhibit separase in mitotic cells. Here, we used a separase biosensor and perturbed the three inhibitory pathways during meiosis I in mouse oocytes. We show that inhibition mediated by either securin or cyclin B1-CDK1, but not SGO2, is independently sufficient to suppress separase activity. However, when both the securin and cyclin B1-CDK1 inhibitory pathways are perturbed together, separase activity begins prematurely, resulting in gross segregation defects. Furthermore, we characterised shugoshin-2 destruction dynamics and conclude that it is not an essential separase inhibitor in mouse oocytes. The existence of multiple separase inhibitory pathways highlights the critical importance of tightly regulated separase activity during this unique and challenging cell division.
Topography of scene memory and perception activity in posterior cortex – a publicly available resource
Authors: Steel Adam , Prasad Deepa , Garcia Brenda D. , Robertson Caroline E. 
Published: January 2025

AbstractAdaptive behavior in complex environments requires integrating visual perception with memory of our spatial environment. Recent work has implicated three brain areas in posterior cerebral cortex — the place memory areas (PMAs) that are anterior to the three visual scene perception areas (SPAs) – in this function. However, PMAs’ relationship to the broader cortical hierarchy remains unclear due to limited group-level characterization. Here, we examined the PMA and SPA locations across three fMRI datasets (44 participants, 29 female). SPAs were identified using a standard visual localizer where participants viewed scenes versus faces. PMAs were identified by contrasting activity when participants recalled personally familiar places versus familiar faces (Datasets 1-2) or places versus multiple categories (familiar faces, bodies, and objects, and famous faces; Dataset 3). Across datasets, the PMAs were located anterior to the SPAs on the ventral and lateral cortical surfaces. The anterior displacement between PMAs and SPAs was highly reproducible. Compared to public atlases, the PMAs fell at the boundary between externally-oriented networks (dorsal attention) and internally-oriented networks (default mode). Additionally, while SPAs overlapped with retinotopic maps, the PMAs were consistently located anterior to mapped visual cortex. These results establish the anatomical position of the PMAs at inflection points along the cortical hierarchy between unimodal sensory and transmodal, apical regions, which informs broader theories of how the brain integrates perception and memory for scenes. We have released probabilistic parcels of these regions to facilitate future research into their roles in spatial cognition.Significance statementComplex behavior requires the dynamic interplay between mnemonic and perceptual information. For example, navigation requires representation of the current visual scene and its relationship to the surrounding visuospatial context. We have suggested that the place memory areas, three brain areas located anterior to the scene perception areas in visual cortex, are well-positioned to serve this role. Here, in a large group of participants, we show that the place memory areas are robustly localizable, and that their position at the interface of multiple distributed brain networks is uniquely suited to mnemonic-perceptual integration. We have released probabilistic regions-of-interest and localization procedure so that others can identify these areas in their own participants.
Engineering surface electrostatics affords control over morphological preference, synergy, and activity in polymer degrading enzymes
Authors: Oliveira Liliana , Rudge Elaine M. , Zahn Michael , Bemmer Victoria , Green Kerry R. , Pickford Andrew R. , Lichtenstein Bruce R. 
Published: January 2025

AbstractThe biocatalytic recycling of plastics, such as polyethylene terephthalate (PET), promises a sustainable alternative to our present open-loop cycles. Engineering of PET-hydrolases for this purpose has focused on improving activity near the glass-transition temperature of the polymer by increasing their thermostability, neglecting other features of the protein-polymer system that affect enzymatic activity. Here, we isolate the effect of electrostatics on the activity of a thermophilic PETase by rationally redesigning its surface charge, while preserving its thermodynamic properties. The enzyme variant,SfInv, shows orders of magnitude improvements in binding affinity and in activity towards untreated plastic films, with inverted morphological preference. When combined, the wildtype enzyme andSfInv act synergistically, revealing an entirely new mechanism for cooperative activities driven by complimentary electrostatic interactions at the PET surface. These findings highlight unexplored avenues in improving PETase function through the control of morphological preference or introduction of protein cooperativity by exploiting protein electrostatics.
Chemical shift and relaxation regularisation improve the accuracy of1H MR spectroscopy analysis
Authors: Wilson Martin 
Published: January 2025

AbstractPurposeAccurate analysis of metabolite levels from1H MRS data is a significant challenge, typically requiring the estimation of approximately 100 parameters from a single spectrum. Signal overlap, spectral noise and common artefacts further complicate analysis, leading to instability and reports of poor agreement between different analysis approaches. One inconsistently used method to improve analysis stability is known as regularisation, where poorly determined parameters are partially constrained to take a predefined value. In this study we examine how regularisation of frequency and linewidth parameters influences analysis accuracy.MethodsThe accuracy of three MRS analysis methods was compaired: 1) ABfit, 2) ABfit-reg and 3) LCModel, where ABfit-reg is a modified version of ABfit incorporating regularisation. Accuracy was assessed on synthetic MRS data generated with random variability in the frequency shift and linewidth parameters applied to each basis signal. Spectra (N=1000) were generated across a range of SNR values (10, 30, 60, 100) to evaluate the impact of variable data quality.ResultsComparison between ABfit and ABfit-reg demonstrates a statistically significant (p <0.0005) improvement in accuracy associated with regularisation for each SNR regime. An approximately 10% reduction in the mean squared metabolite errors were found for ABfit-reg compared to LCModel for SNR>10 (p <0.0005). Furthermore, Bland-Altman analysis shows that incorporating regularisation into ABfit enhances its agreement with LCModel.ConclusionRegularisation is beneficial for MRS fitting and accurate characterisation of the frequency and linewidth variabilityin vivomay yield further improvements.
In vivomeasurements of receptor tyrosine kinase activity reveal feedback regulation of a developmental gradient
Authors: Ho Emily K. , Kim-Yip Rebecca P. , Simpkins Alison G. , Farahani Payam E. , Oatman Harrison R. , Posfai Eszter , Shvartsman Stanislav Y. , Toettcher Jared E. 
Published: January 2025

AbstractA lack of tools for detecting receptor activityin vivohas limited our ability to fully explore receptor-level control of developmental patterning. Here, we extend a new class of biosensors for receptor tyrosine kinase (RTK) activity, the pYtag system, to visualize endogenous RTK activity inDrosophila. We build biosensors for threeDrosophilaRTKs that function across developmental stages and tissues. By characterizing Torso::pYtag during terminal patterning in the early embryo, we find that Torso activity differs from downstream ERK activity in two surprising ways: Torso activity is narrowly restricted to the poles but produces a broader gradient of ERK, and Torso activity decreases over developmental time while ERK activity is sustained. This decrease in Torso activity is driven by ERK pathway-dependent negative feedback. Our results suggest an updated model of terminal patterning where a narrow domain of Torso activity, tuned in amplitude by negative feedback, locally activates signaling effectors which diffuse through the syncytial embryo to form the ERK gradient. Altogether, this work highlights the usefulness of pYtags for investigating receptor-level regulation of developmental patterning.
Using magnetoencephalography to track the propagation of 40 Hz invisible spectral flicker
Authors: Henney Mark Alexander , Spaak Eelke , Hansen Henrik Enggaard , Carstensen Marcus , Madsen Kristoffer Hougaard , Oostenveld Robert 
Published: January 2025

AbstractBrain stimulation with novel 40 Hz invisible spectral flicker (ISF) has been proposed as a therapy for Alzheimer’s disease, with leading hypotheses suggesting local promotion of glymphatic clearance of amyloid as the mechanism of action. Neural signals in the gamma range and their spatial propagation over the brain can be tracked using magnetoencephalography (MEG) with high temporal and good spatial detail. However, stimulation with 40 Hz ISF requires specialised hardware which causes electromagnetic interference (EMI) with MEG equipment.MEG measures the tiny magnetic fields of the brain, which are easily distorted by external magnetic fields. Using MEG to track the propagation of 40 Hz ISF requires multiple modifications to the experimental setup. These include, at least 1) an experimental design that promotes modulation of the neural signal, but not the artifact, 2) removal of all electronics not strictly necessary for light production from the stimulators, and 3) signal processing for 40 Hz EMI artifact suppression.Here, we present an MEG study on the cortical propagation of 40 Hz ISF. In two experiments, we investigate the modulation that visual and non-visual cognitive tasks have on the power and propagation of cortical activity induced by 40 Hz ISF.With the chosen experimental setup and design, the 40 Hz EMI artifact could not be entirely disentangled from the neural signal of interest, thus rendering inference on the spatial propagation of the 40 Hz signal impossible. Further improvements need to be implemented in a follow-up experimental design. We present potential solutions to allow for future investigation of 40 Hz ISF with MEG.
Power loss analysis for the optical cavity of an x-ray laser oscillator
Authors: Tiwari Ganesh , Yu Li Hua , Yang Xi , Shaftan Timur , Smaluk Victor 
Published: January 2025

We investigate properties of optical elements in the optical cavity of an x-ray laser oscillator with emphasis on the power loss of a monochromatic Gaussian radiation beam upon passing through them using analytical and numerical approaches. Here we assume the optical cavity comprises of refractive lenses and Bragg crystals exploiting symmetric Bragg scattering. For the lens, we include focusing and curvature effects while we include angular filtering due to finite Darwin width of the Bragg crystal. Our results indicate the feasibility of x-ray power out-coupling in the range of 0.5%–28% with intracavity reflectivity of 67%–95% at 10.321 keV for nominal (≥65% reflectivity) cases. We also show the possibility of achieving high out-coupling of 35%–80% while restricting the intracavity reflectivity in the 19%–62% by adopting lossy cavities with 10–20  μm thin crystals.

Published by the American Physical Society
2025
Negative emotions’ impact on consumer satisfaction and loyalty due to failures in financial services: application of the affective-cognitive model
Authors: Sottolichio Alejandro J. , Ponce Hector R. , Rojas Cabezas Germán 
Published: January 2025

PurposeWe examine negative emotions’ influence on consumer satisfaction and loyalty when repurchasing a product or service after experiencing failures in the financial services sector.Design/methodology/approachThe sample comprised 735 valid surveys of customers who encountered such service failures. An initial model incorporating 14 negative emotions was narrowed down to the most robust one, comprising three emotions, after data collection and statistical validation.FindingsConsumer dissatisfaction is explained by affective (pleasure) rather than cognitive factors (disconfirmation) in the financial services context. Loyalty is influenced only by affective (pleasure and activation) rather than cognitive elements (disconfirmation) in the dissatisfaction generation process, indicating that loyalty is not a cognitive but an affective one. Finally, an affective judgment (activation) rather than a cognitive one (disconfirmation) explains consumer’s recommendation of a product or service despite encountering failures.Originality/valueThis is the first study to focus exclusively on negative emotions, revealing that the pleasure dimension is a significant antecedent of dissatisfaction. By demonstrating that emotional factors rather than cognitive assessments dominate both satisfaction and loyalty responses, this study offers a unique contribution to understanding consumer behavior after service failures in financial services, with practical implications for service recovery strategies.
Role of supply chain resources capabilities on enhancing innovation-based supply chain risk management
Authors: Huma Sehrish , Siddiqui Danish Ahmed 
Published: January 2025

PurposeThe purpose of this study is to investigate the roles of potential absorptive capacity (PACAP) and realized absorptive capacity (RACAP) in driving different types of innovation practices, including process, product, marketing and organizational innovation with in the supply chain (SC). Furthermore, this study also seeks to examine the previously unexplored relationship between these SC innovation practices and supply chain risk management (SCRM).Design/methodology/approachData were collected from 267 SC professionals working in the export manufacturing industry for hypotheses testing via structural equation modeling.FindingsThe results have found that both PACAP and RACAP positively and significantly impact on each practice of SC innovation. The results also show that each SC innovation practices such as product innovation, process innovation and marketing innovation have a positive and significant impact on SCRM except organizational innovation.Originality/valueThis study makes a notable contribution to the broader SC management literature, with a specific focus on absorptive capacity, innovation management and SCRM, areas where empirical research is still emerging. This study reveals that organizations trying to manage SC risk must implement sufficient SC innovation practices by enhancing their PACAP and RACAP for quick response and recovery from SC disruptions.
The impact of the pandemic on management in public sector organisations
Authors: Meierdirk Charlotte , Fleischer Stephanie 
Published: January 2025

PurposeThis study investigates management challenges, communication plans and crisis decision-making during the COVID-19 pandemic, plus highlights management excellence for future operations. Using Deloitte Consultancy’s (2015) time frames of organisational crisis management strategies: response, recovery and readiness, this article explores management approaches of the UK’s public sector organisations (PSO) during the COVID-19 pandemic.Design/methodology/approachA mixed-method approach consisting of a quantitative survey followed by semi-structured interviews was used. About 33 part-time master’s degree students on a business administration (MBA) course, who were also full-time managers in the UK’s healthcare, education and other PSOs, completed the survey. The survey responses were subsequently used to select nine participants for follow-up semi-structured online interviews.FindingsQualitative data showed the importance of speedy decisions and clarity of communication during the pandemic. Teamwork was seen as paramount to successful decision-making despite many changing job roles. On reflection, managers gained confidence in future crisis decision-making with the importance of sharing ideas between departments, but further reflection was needed to prepare for future challenges.Research limitations/implicationsOne of the limitations to this research is that the participants are all part-time MBA students, and although they were all full-time managers in PSOs, they may be more critical of the status quo.Practical implicationsPractically, organisations would benefit from reflecting on their reaction to the pandemic and what can be learnt. It is clear the majority of the managers interviewed felt that after the pandemic the organisations just moved on and there was no in-depth reflection on what had happened; the last stage of Deloitte’s (2015) model was never fully realised. The post-pandemic phase is an area that would benefit from further research, as it has now been over four years since the beginning of the pandemic, and some of the positives, such as teamwork and a sense of belonging, seem to have been lost. The pandemic has left organisations with staff absences and long-term illnesses; last year alone workplace absences cost the UK economy £32.7bn (Nolsoe, 2014). To survive, organisations have to be resilient, responsive and flexible. The next challenge will emerge from navigating times of austerity and the need for PSOs to restructure post-pandemic, an area that would benefit from further research.Originality/valueThe COVID-19 pandemic was a crisis that impacted all organisations, including their goals, operations and employees. It was clear that the amount of planning was vast, but as soon as the pandemic was over, quite a few of the organisations went back to the status quo and the “readiness stage” did not happen. The smaller organisations were more likely to reflect and improve than the larger ones.
Exploring the nexus between tourist characteristics and social media’s impact on decision-making: a PLS-MGA based study of Indian tourists
Authors: Gulati Shruti , Kumar Suneel 
Published: January 2025

Purpose
This study aims to explore the nexus between tourist’s characteristics and the changing intensity of social media’s impact on tourist decision-making among Indian tourists. A group-based comparison is done in this exploratory study; the basis of groups is the tourist’s characteristics which signifies their different types such as social media type, user type, traveller type and destination type. India, which is a huge market for tourists and social media users, is explored for this study.

Design/methodology/approach
This study adopts an exploratory design where an attempt is made to analyse if the intensity of social media impact on their decision-making changes with different types of tourists. For this purpose, primary data analysis using Partial Least Squares Regression based Multi Group Analysis (PLS-MGA) is performed on 488 Indian Tourists which was collected using a structured online questionnaire. The groups have been divided according to tourist characteristics signifying different types like social media type, user type, traveller type and destination type.

Findings
Social media usage frequency and travel frequency have no moderating effect on social media’s impact on tourist decision-making. But social media type partially moderates decision-making with changing impact on the need recognition, information search and post purchase behaviour stages. Destination type also partially moderates with an impact on post purchase behaviour stage of tourist decision-making. Thus, it can be said that different types of tourists are impacted differently by social media across various stages of decision-making.

Originality/value
This is a novel study that for the first time explores the changes in the impact of social media on tourist decision-making through changes in their own personal characteristics. Also, it provides real-time insights for tourism marketing for Indian market which has huge potential due to Indians being avid travellers and users of social media.
Health tourism in the era of COVID-19: a bibliometric analysis
Authors: Kulkarni Darshana Girish , Kulkarni Girish Ramesh , Saurabh Punit , Shome Samik 
Published: January 2025

Purpose
The COVID-19 outbreak has severely impacted the health tourism industry. This study aims to examine the research undertaken on health tourism during COVID-19.

Design/methodology/approach
A bibliometric analysis was performed using 211 documents written by 645 authors between 2020 and 2024. The databases used for this analysis are SCOPUS, Web of Science and PubMed. The study was conducted using the Biblioshiny application for R software. Various analyses, including citation analysis, keyword analysis, thematic mapping and evolution and collaboration analysis, were conducted to get a comprehensive picture of the impact of COVID-19 on health tourism.

Findings
The findings suggest that medical tourism was the major area of focus for researchers during COVID-19, whereas wellness tourism was studied in the later stages of the pandemic and is considered important for coping with the negative effects of COVID-19. The thematic review indicates that pre-pandemic research focused on strategies that accelerate the growth of the health tourism sector, whereas during and post-pandemic, the focus was on studying the impact of COVID-19 on the survival and recovery of the sector. Overall, health tourism was considered instrumental in the post-pandemic revival of the tourism industry.

Research limitations/implications
The bibliometric analysis is limited to only four years, as the focus was on studying health tourism during COVID-19. Hence, the study offers more precise insights into the research focus on health tourism during a pandemic. The current study offers limited insights into the post-pandemic scenario as the research is still emerging.

Practical implications
There is a significant opportunity for future research on post-COVID-19 recovery strategies, wherein this research will be helpful for researchers, particularly from emerging countries and countries with high dependence on health, medical and wellness tourism. The thematic analysis provides a valuable comparison of pre-, during and post-pandemic research.

Social implications
The research indicates an opportunity to study the policies and strategies adopted by various countries regarding the health tourism sector in the post-COVID-19 era. The research also highlights the interwoven, intricate, interdisciplinary nature of health tourism in relation to public and mental health and the need for collaborative efforts.

Originality/value
The study provides critical insights and new directions for future research by highlighting the crucial role of health tourism in mitigating the impact of COVID-19.