bluuebunny/crossref_metadata_2025_split huggingface dataset:
Effect of olive‐processing technology on the utilization of olive mill pomace as a soil amendment
Authors: Černe Marko , Palčić Igor , Major Nikola , Pasković Igor , Perković Josipa , Užila Zoran , Lukić Marina , Romić Marija , Zubin Ferri Tea , Goreta Ban Smiljana , Lapanje Aleš , Hreščak Jitka , Heath David John , Ban Dean 
Published: February 2023

AbstractIn the Mediterranean basin, the treatment and disposal of olive mill pomace (OMP) remain a salient environmental issue for the olive oil‐producing industry. This study assesses the effects of olive‐processing technology (three‐phase and two‐phase systems) on the potential use of OMP as a soil amendment. Samples from 12 Croatian olive mills were analyzed for their total phenolic content (TPC), residual oil fraction, and elemental concentration. The samples were profiled using Fourier transform infrared spectroscopy (FT‐IR) and structurally characterized using scanning electron microscopy‐energy‐dispersive X‐ray spectroscopy (EDS). Compared to three‐phase samples, two‐phase OMP was more acidic (pH 4.5 vs. 5.0), with a higher TPC (3835 vs. 1576 mg/kg fresh weight), oil content (11.7% vs. 7.5% d.w., where d.w. is dry weight), electrical conductivity (EC, 5.1 vs. 3.0 mS/cm), and levels of calcium (Ca, 1.34 vs. 1.20 g/kg d.w.) and copper (Cu, 10.4 vs. 7.0 mg/kg d.w.). Similar values of carbon/nitrogen (C/N; 61 vs. 72), N (10 vs. 8.1 g/kg d.w.), phosphorus (1040 vs. 691 mg/kg d.w.), and potassium (K, 13.7 vs. 8.1 g/kg d.w.) were observed. The amounts of chromium, copper, nickel, and zinc were below EC limits in both cases. The EDS mapping revealed that Ca was concentrated at sharp‐edged OMP particles while K was evenly distributed, suggesting that pelletized OMP compost is preferable for amending soil to obtain a homogeneous distribution of nutrients. It was also possible to distinguish between OMPs based on oil and lignin absorption bands in their FT‐IR spectra. According to the obtained results, composting is recommended for both types of OMP to produce a safe product for amendment purposes.
Application of CAR‐T cell technology in autoimmune diseases and human immunodeficiency virus infection treatment
Authors: Adabi Nasim , Pordel Safoora , Rezaee Mohammad Ali , Shobeiri Fatemeh Sadat , Shobeiri Saeideh Sadat 
Published: February 2023

AbstractChimeric antigen receptor (CAR) T‐cell therapy is an immunotherapy approach that has played a tremendous role in the battle against cancer for years. Since the CAR T lymphocytes are unrestricted‐major histocompatibility complex T lymphocytes, they could identify more targets than natural T cells, resulting in practical and widespread functions. The good prospects of CAR T‐cell therapy in oncology can be additionally applied to treat other diseases such as autoimmune and infectious diseases. CAR‐T cell‐derived immunotherapy for autoimmune disorders can be allocated to CAR‐Tregs and chimeric autoantibody receptor T cells. Other generations of CARs target human immunodeficiency virus (HIV) proteins. In this review, we summarize CAR‐T cell therapies in autoimmune disorders and HIV infection.
Alcohol use, suicidality and virologic non‐suppression among young adults with perinatally acquired HIV in Thailand: a cross‐sectional study
Authors: Aurpibul Linda , Kosalaraksa Pope , Kawichai Surinda , Lumbiganon Pagakrong , Ounchanum Pradthana , Natalie Songtaweesin Wipaporn , Sudjaritruk Tavitiya , Chokephaibulkit Kulkanya , Rungmaitree Supattra , Suwanlerk Tulathip , Ross Jeremy L. , Sohn Annette H. , Puthanakit Thanyawee ,   the Thai PAPAYA study team
Published: February 2023

AbstractIntroductionYoung adults with perinatally acquired HIV (YA‐PHIV) are facing transitions to adult life. This study assessed health risk behaviours (including substance use), mental health, quality of life (QOL) and HIV treatment outcomes of Thai YA‐PHIV.MethodsA cross‐sectional study was conducted in Thai YA‐PHIV aged 18–25 years who were enrolled in a prospective cohort study at five tertiary paediatric HIV care centres in Thailand. Study data were obtained through face‐to‐face interviews from November 2020 to July 2021. Assessments were performed for alcohol use (Alcohol Use Disorders Identification Test; AUDIT), smoking (Fagerstrom Test for Nicotine Dependence), drug/substance use (Drug Abuse Screening Test; DAST‐10), depression (Patient Health Questionnaire for Adolescents; PHQ‐A), anxiety (Generalized Anxiety Disorder; GAD‐7) and QOL (World Health Organization QOL Brief‐Thai). HIV treatment outcomes were extracted from the National AIDS Program database.ResultsOf 355 YA‐PHIV, 163 (46%) were males: their median age was 21.7 (interquartile range, IQR 20.2–23.5) years. There were 203 YA‐PHIV (58%) who reported ever having sex; 141 (40%) were sexually active in the past 6 months, of whom 86 (61%) reported 100% condom use. Overall, 49 (14%) met the criteria for harmful alcohol use; 28 (7.9%) were alcohol dependent. Sixty (17%) were current smokers and 37 (11%) used drugs/substances. The frequency of moderate up to severe symptoms for depression was 18% and for anxiety was 9.7%. Their overall QOL was good in 180 (51%), moderate in 168 (47%) and poor in five (1.4%). There were 49 YA‐PHIV (14%) with CD4 <200 cells/mm3 and 85 (24%) with virologic non‐suppression (HIV‐RNA >200 copies/ml). On multivariate analyses, the highest education at the primary to high school or vocational school levels (adjusted odds ratio [aOR] 2.02, 95% CI 1.40–3.95, p 0.04), harmful alcohol use (aOR 2.48, 95% CI 1.24–4.99, p 0.01), alcohol dependence (aOR 3.54, 95% CI 1.51–8.31, p <0.01) and lifetime suicidal attempt (aOR 2.66, 95% CI 1.11–6.35, p 0.03) were associated with non‐suppression.ConclusionsRegular screening for alcohol use and mental health, including suicidality, would be useful to identify YA‐PHIV who need more intensive psychosocial support or referral services to ensure they can achieve and maintain a high QOL into adult life.
Intra‐ and Inter‐visit Repeatability of 129Xenon Multiple‐Breath Washout MRI in Children With Stable Cystic Fibrosis Lung Disease
Authors: Alam Faiyza S. , Zanette Brandon , Munidasa Samal , Braganza Sharon , Li Daniel , Woods Jason C. , Ratjen Felix , Santyr Giles 
Published: February 2023

BackgroundMultiple‐breath washout (MBW) 129Xe MRI (MBW Xe‐MRI) is a promising technique for following pediatric cystic fibrosis (CF) lung disease progression. However, its repeatability in stable CF needs to be established to use it as an outcome measure for novel therapies.PurposeTo assess intravisit and intervisit repeatability of MBW Xe‐MRI in healthy and CF children.Study TypeProspective, longitudinal cohort study.SubjectsA total of 18 pediatric subjects (7 healthy, 11 CF).Field Strength/SequenceA 3 T/2D coronal hyperpolarized (HP) 129Xe images using GRE sequence.AssessmentAll subjects completed MBW Xe‐MRI, pulmonary function tests (PFTs) (spirometry, nitrogen [N2] MBW for lung clearance index [LCI]) and ventilation defect percent (VDP) at baseline (visit 1) and 1‐month after. Fractional ventilation (FV), coefficient of variation (CoVFV) maps were calculated from MBW Xe‐MRI data acquired between intervening air washout breaths performed after an initial xenon breath‐hold. Skewness of FV and CoVFV map distributions was also assessed.Statistical TestsRepeatability: intraclass correlation coefficients (ICC), within‐subject coefficient of variation (CV%), repeatability coefficient (CR). Agreement: Bland–Altman. For correlations between MBW Xe‐MRI, VDP and PFTs: Spearman's correlation. Significance threshold: P < 0.05.ResultsFor FV, intravisit median [IQR] ICC was high in both healthy (0.94 [0.48, 0.99]) and CF (0.83 [0.04, 0.97]) subjects. CoVFV also had good intravisit ICC in healthy (0.92 [0.42, 0.99]) and CF (0.79 [0.02, 0.96]) subjects. Similarly, for FV, intervisit ICC was high in health (0.94 [0.68, 0.99]) and CF (0.89 [0.61, 0.97]). CoVFV also had good intervisit ICC in health (0.92 [0.42, 0.99]) and CF (0.78 [0.26, 0.94]). FV had better intervisit repeatability than VDP. CoVFV correlated significantly with LCI (R = 0.56). Skewness of FV distributions significantly distinguished between cohorts at baseline.Data ConclusionMBW Xe‐MRI had high intravisit and intervisit repeatability in healthy and stable CF subjects. CoVFV correlated with LCI, suggesting the importance of ventilation heterogeneity to early CF.Evidence Level1.Technical EfficacyStage 2.
Head‐to‐head comparison of genotyping of human papillomavirus by real‐time multiplex PCR assay using type‐specific primers and SPF10‐PCR‐based line probe assay
Authors: Yin Jian , Peng Siying , Zhang Changning , Li Xinyue , Hu Fangfang , Chen Wen , Qiao Youlin 
Published: February 2023

AbstractThe SPF10‐polymerase chain reaction (PCR)‐based line probe assay (LiPA‐25) with high analytical sensitivity and specificity for human papillomavirus (HPV) genotyping in clinical samples has been widely used in vaccine and epidemiologic studies. A real‐time multiplex PCR assay using type‐specific primers (Hybribio‐23) with low workload and cost has been developed recently. The study aimed to compare the performance of LiPA‐25 and Hybribio‐23 in selected 1731 cervical swab and 117 tissue samples, with a focus on 20 common HPV types (14 high‐risk: 16, 18, 31, 33, 35, 39, 45, 51, 52, 56, 58, 59, 66, and 68/73; 6 low‐risk: 6, 11, 42, 43, 44, and 53). The level of agreement of two assays was determined using Cohen's Kappa (κ) statistics. A total of 1296 (74.9%) swab samples were identified as HPV‐positive by Hybribio‐23 or LiPA‐25, of which 814 (62.8%) samples exhibited concordant, 358 (27.6%) showed additional or fewer types (compatible), and 124 (9.6%) were discordant. In addition, the two assays showed a perfect agreement for 20 HPV‐combined detection (κ = 0.838) and 17 individual HPV types (all κ > 0.800), a good agreement for HPV31 (κ = 0.792) and 43 (κ = 0.696), and a moderate agreement for HPV42 (κ = 0.504). Hybribio‐23 was significantly more sensitive for HPV58, 59, 68/73, 42, 43, and 44, and less sensitive for HPV35 and 66 than LiPA‐25 (McNemar's test: all p < 0.05). For 117 HPV‐positive tissue specimens, the identification of genotypes was 85.2% identical, 12.2% compatible, and only 2.6% discordant. The agreement for HPV31 (κ = 0.786), 68/73 (κ = 0.742), and HPV53 (κ = 0.742) was good, while for other types (all κ > 0.853) and 20 HPV‐combined detection (κ = 0.936) was perfect (all p > 0.05). In conclusion, Hybribio‐23 and LiPA‐25 are comparable. Hybribio‐23 could be used for the detection and genotyping of HPV in cervical samples for epidemiological and vaccine studies worldwide.
Promotion and caution in research article abstracts: The use of positive, negative and hedge words across disciplines and rankings
Authors: Xie Shaoliang , Mi Chenggang 
Published: February 2023

AbstractAbstract as a promotional genre has been an increasing interest in recent years, leading to an intriguing debate on the objectivity of scientific writing. The present study investigated the promotion and caution in research article abstracts through the use of positive, negative and hedge words across disciplines and rankings based on a large and principled dataset (more than 12.6 million words). The corpus was designed and built with full consideration of representativeness, structure, balance, and size in terms of discipline and ranking. The results showed that positive words were more frequently used than negative words in terms of both discipline and ranking, positive and negative words were more frequently used in hard sciences than those in soft sciences and high ranking journals than those in low ranking journals, and hedge words were more frequently used in high ranking journals and soft sciences. Further investigation also found a complexity of frequency patterns when two disciplines were broken down into specific categories. The more frequent use of positive words in abstracts to promote scientific research was discussed from the perspective of disciplinary knowledge construction in addition to the publication bias, the outcome reporting bias, and universal linguistic positivity bias.
Bilirubin Removal by Polymeric Adsorbents for Hyperbilirubinemia Therapy
Authors: Wang Yilin , Wei Ran , Zhao Weifeng , Zhao Changsheng 
Published: February 2023

AbstractHyperbilirubinemia, presenting as jaundice, is a life‐threatening critical illness in newborn babies and acute severe hepatic failure patients. Over the past few decades, extracorporeal hemoadsorption by adsorbent therapy has been widely applied in the treatment of hyperbilirubinemia. The capability of hemoadsorption depends on the adsorbents. Most of the clinically used bilirubin adsorbents are made up of styrene/divinylbenzene copolymer and quaternary ammonium salt, which usually have poor biocompatibility and weak mechanical strength. To overcome the drawbacks of commercial polymer adsorbents, advanced synthetic and natural polymers with/without nanomaterials have been designed, and novel adsorbent fabrication technologies have also been developed. In this review, the adsorption mechanism of bilirubin adsorbents has been summarized, which is the basic criterion in adsorbent development. Furthermore, the preparation method, adsorption mechanism, relative merits and practicability of the emerging bilirubin adsorbents have been evaluated. Based on the existing studies, this work highlights the future direction of the efforts on how to design and develop bilirubin adsorbents with good overall clinical performance. Perhaps this study can change traditional perspectives and propose new strategies for bilirubin clearance from the aspects of pathogenic mechanisms, metabolic pathways, and material‐based innovation.
Plasma Biomarkers and Positron Emission Tomography Tau Pathology in Progressive Supranuclear Palsy
Authors: Huang Shu‐Yi , Chen Shu‐Fen , Cui Mei , Zhao Meng , Shen Xue‐Ning , Guo Yu , Zhang Ya‐Ru , Zhang Wei , Wang Hui‐Fu , Huang Yu‐Yuan , Cheng Wei , Zuo Chuan‐Tao , Dong Qiang , Yu Jin‐Tai 
Published: February 2023

ABSTRACTBackgroundDevelopment of disease‐modifying therapeutic trials of progressive supranuclear palsy (PSP) urges the need for sensitive fluid biomarkers.ObjectivesThe objectives of this study were to explore the utility of plasma biomarkers in the diagnosis, differential diagnosis, and assessment of disease severity, brain atrophy, and tau deposition in PSP.MethodsPlasma biomarkers were measured using a single‐molecule array in a cohort composed of patients with PSP, Parkinson's disease (PD), multiple system atrophy with predominant parkinsonism (MSA‐P), and healthy controls (HCs).ResultsPlasma neurofilament light chain (NfL) outperformed other plasma makers (ie, glial fibrillary acidic protein [GFAP], phosphorylated‐tau 181 [p‐tau181], amyloid‐β 1–40, amyloid‐β 1–42) in identifying PSP from HC (area under the curve [AUC] = 0.904) and from MSA‐P (AUC = 0.711). Plasma GFAP aided in distinguishing PSP from HC (AUC = 0.774) and from MSA‐P (AUC = 0.832). It correlated with brainstem atrophy and higher regional tau accumulation. However, plasma p‐tau181 neither helped in diagnosis nor was it associated with clinical or neuroimaging measures.ConclusionsPlasma NfL and GFAP showed different values in differentiating PSP from HC or controls with other forms of neurodegenerative parkinsonism and detecting disease severity, brain atrophy, or tau deposition in PSP. © 2023 International Parkinson and Movement Disorder Society.
Incidence and genetic variants of inborn errors of metabolism identified through newborn screening: A 7‐year study in eastern coastal areas of China
Authors: Men Shuai , Liu Shuang , Zheng Qin , Yang Shuting , Mao Huafen , Wang Zhiwei , Gu Ying , Tang Xinxin , Wang Leilei 
Published: February 2023

AbstractBackgroundThe incidence of inborn errors of metabolism (IEM) varies across countries and areas. Currently, there are no studies on IEM using newborn screening (NBS) in eastern coastal areas of China. We aimed to estimate the incidence and genetic variants of IEM and understand the spectrum of diseases caused by IEM and variants among them in this area.MethodsThe NBS performed by tandem mass spectrometry (MS/MS) from 2016 to 2021 was retrospectively reviewed. Heel blood was collected from all newborns 72 h after birth. Targeted massively parallel sequencing was performed for genetic analysis.ResultsAmong 245,194 newborns, 95 were diagnosed with IEM, the overall incidence observed was—IEM: 1/2581; amino acid metabolism disorder: 1/4715; organic acid metabolism disorder: 1/11676; and fatty acid metabolism disorder: 1/11145. The incidence of different IEM was in the range of 1/245194 to 1/6452. Phenylketonuria (PKU, 1/7211) was the most common IEM, followed by methylmalonic acidemia (MMA, 1/27244), short‐chain acyl‐CoA dehydrogenase deficiency (SCADD, 1/30649), and citrin deficiency (CD, 1/35028). For genetic variants, the common hotspot variants found were—PAH gene for PKU: c.728G > A, c.442‐1G > A, c.611A > G, c.721C > T; PTS gene for non‐classical PKU: c.259C > T; MMACHC gene for MMA: c.658_660delAAG, c.609G > A; MMUT gene for MMA: c.1663G > A; ACADS gene for SCADD: c.1031A > G and c.1130C > T; and SLC25A13 gene for CD: c.1638_1660dup, c.852_855del.ConclusionThis study displayed the diseases and varied spectrum of IEM in eastern coastal areas of China. Implementing NBS for IEM by MS/MS combined with massively parallel sequencing can offer an improved plan for NBS to detect IEM.
Plant blindness represents the loss of generational knowledge and cultural identity
Authors: Blue Stacie , Hargiss Christina L. M. , Norland Jack , Dekeyser Edward S. , Comeau Paula 
Published: February 2023

AbstractPlant blindness, the inability of people to notice plants, is of current interest in the natural sciences community. It has been hypothesized that communities of varying cultures may have improved plant sight. Researchers used an online survey to assess citizens ability to identify plants. The survey also intended to address the concern of elders from the Turtle Mountain Band of Chippewa Indians (TMBCI) who have seen reduced abundance of traditional plant species. The survey assessed knowledge of local wetland, grassland, and forest habitats; names of plants; and how citizens use plant features in identification. The survey gathered demographic data to determine trends across different demographic groups including age and ethnicity. A total of 216 participants took the survey, the majority classified themselves as female and Native American or Alaska Native. Our findings indicated that participants were able to identify forest and wetland plants the best, but struggled with grassland plants. Older individuals were more able to identify plants when compared to younger individuals. Ecologically, increasing plant knowledge is vital to rebuilding and maintaining the diversity of local habitats, but culturally the impacts are just as important. The practice of using traditional plants in medicine and ceremony is an integral part of the TMBCI culture. As both the knowledge and the plants disappear in younger generations, so does their unique cultural identity.
High‐strength/high‐modulus polyvinyl alcohol fiber reinforced poly(amino acid)/hydroxyapatite composite for load‐bearing orthopedics applications
Authors: Wu Yanan , Lyu Defu , Chen Lichao , Ren Haohao , Yan Yonggang 
Published: February 2023

AbstractBone defects in the load‐bearing area require bone reconstruction using strong biomaterial with mechanical properties like cortical bone. Herein, the high‐strength/high‐modulus polyvinyl alcohol fiber (PVA) was first designed to incorporate into poly(amino acid) (PAA)/hydroxyapatite (HA) to obtain a new poly(amino acid)/hydroxyapatite/polyvinyl alcohol fiber (PAA/HA/PVA) composite via a melt extrusion process. To improve the interfacial adhesive performance, the titanate coupling agent HY‐109 [isopropyl tris(dodecylbenzenesulfonyl) titanate] was used for simultaneously graft modification of the PVA fiber and HA (TPVA, THA). The effect of their components on the mechanical properties and degradation ability were investigated by electromechanical universal testing machine and in vitro phosphate buffer solution soaking. The results indicated that the PAA/30THA/20TPVA composite had the most ideal mechanical and degradation properties, which far exceed those of PAA and PAA/THA composite. In addition, in vitro simulated body fluid immersion and cell culture experiment showed the PAA/THA/TPVA composites boosted in vitro bioactivity and exhibited excellent cytocompatibility and osteogenic activity in cell attachment, proliferation, alkaline phosphatase activity, matrix mineralization, and osteogenesis‐related gene expression. These reports demonstrated that the developed PAA/THA/TPVA composite displayed comprehensive performance optimizations, which showed its potential as a novel biomaterial to be applied as load‐bearing implant in the field of orthopedics.
Bi‐homogeneous observers for uncertain 1‐DOF mechanical systems
Authors: Texis‐Loaiza Oscar , Moreno Jaime A. , Fridman Leonid 
Published: February 2023

AbstractA global, bi‐homogeneous, observer for a class of 1‐DOF mechanical systems with Coriolis and centrifugal forces, dry and viscous frictions and non‐vanishing bounded uncertainties/perturbations is proposed, ensuring predefined upper bound of convergence time. The options for convergence acceleration are discussed in a simulation example.
Co‐Assembly of Peptide with G‐Quadruplex DNA: A Strategic Approach to Develop Anticancer Therapeutics
Authors: Biswas Soumi , Basak Shubhanwita , Samui Satyabrata , Pasadi Sanjeev , Muniyappa K. , Naskar Jishu 
Published: February 2023

AbstractG‐quadruplex (G4) is one of the non‐canonical nucleic acid structures that regulate multiple key biological processes. The formation of G4 hinders the progression of cancer. The molecular entities which can stabilize the G4 structure are very important to design anticancer therapeutics. Herein, it is described that a synthetic dendron‐like peptide, Cδ3‐(YYEE)‐E co‐assembles with G4 DNA in aqueous buffer containing Na+ ions. Various orthogonal biophysical studies have established the co‐assembly phenomenon. In silico, molecular docking studies also corroborate the results obtained from biophysical experiments. Importantly, the co‐assembly enhances the thermal stability of G4 DNA compared to free G4 DNA. The peptide inhibits the activity of telomerase enzyme which is found to be over‐expressed in most of the cancer cells and also down‐regulates the c‐Myc oncogenic expression. The peptide exhibits significant cytotoxic effect on cancer cells (HeLa and U2OS) compared to non‐cancer cells (HEK293) under similar experimental conditions. The study highlights the applicability of a synthetic bioactive peptide as a putative anticancer therapeutic.