bluuebunny/crossref_metadata_2025_split huggingface dataset:
A study of criteria for air cargo terminal classification model
Authors:   , Rodbundith Treephis 
Published: September 2023

Global air cargo transportation has performed a significant role to the trade industry over the past decades for goods delivery. Airlines transport approximately 51.3 million metric tons of goods, or more than one third of worldwide trade or USD 6.8 trillion by value annually. Air cargo terminal is a key success of airlines in the supply chain network at airports. This study is aimed to explore and analyze important criteria of air cargo terminals on the integration of 63 criteria from previous researches of practical operations and International Air Transport Association regulated standard services. To examine reviewed criteria, this paper investigates the criteria significance by 57 of 75 air cargo terminal operators around the world except in Thailand covering 474 air cargo terminals in 6 continents. Also, this research cooperatively evaluates the criteria and employs Factor Analysis by using Principal Component Analysis program for criteria reduction with the similarity. The result of final validation from aviation experts, academicians, government bodies, air cargo logistics service providers and airlines in air cargo related industries reveal that 9 integrated criteria with 44 sub-criteria and factors loading scores are remained important. The significant criteria are studied with air cargo terminal operators in Suvarnabhumi, Chiang Mai, Don Muang and Phuket international airports as a sampling to testify the criteria and model from the first phase finding. There are 5 of 7 operators who participate this assessment only in Suvarnabhumi, Chiang Mai and Phuket international airports. Raw data is given and input in Hierarchical Cluster Analysis program to cluster all 5 air cargo terminal operators into 2, 3 and 4 classifications homogenously. Thai Airways as an air cargo terminal operator in Suvarnabhumi international airport is clustered separately in one classification and obviously outstanding from the other 4 air cargo terminal operators and all classification are presented. Still, strength and weakness of Thai Airways in each criteria and all 9 main criteria are analyzed as results from second phase finding of this research and compared with the best practice of air cargo airport namely Hong Kong international airport. Also, this is proven that the significant criteria are workable and practical for air cargo terminal classification model as a new body of knowledge for third phase finding. Later, the 9 main criteria with 44 sub-criteria and factor loading scores are constructed into the guideline and check list that are available for academicians, air cargo terminal operators, government bodies and interested stake holders around the world to prepare and apply to their business and academic purposes.
Reactive oxygen species-induced global DNA hypomethylation in bladder cancer is mediated via increased formation of 8-hydroxy-2'-deoxyguanosine
Authors:   , Whongsiri Patcharawalai 
Published: September 2023

Oxidative stress, as a consequence of the elevated reactive oxygen species (ROS), has been implicated in various chronic diseases including cancers by, for examples, causing oxidative DNA damage and altering epigenetic regulation. Long interspersed element-1 (LINE-1) is an only active retrotransposable elements in human DNA, comprising up to 17% of the whole genome. LINE-1 hypomethylation is reported in bladder cancer and evidently it is a consequence of oxidative stress. In this study, we investigated the expression of 5-methylcytosine (5-mC), 8-hydroxydeoxyguanosine (8-OHdG), LINE-1-endoced protein (ORF1p), histone H3K9me3 and HP1 alpha in bladder cancer tissues. Mechanistic insight into how oxidative stress affected DNA methylation and histone modifications that further caused aberrant expression of LINE-1 elements in bladder cancer cells was also explored. Elevated expression of 8-OHdG, decreased 5-mC expression, upregulation of ORF1p expression, and increase in histone H3K9me3 expression were observed in bladder cancer tissues, indicating increases in oxidative stress and epigenetic alterations. The in vitro study in bladder cancer cell lines revealed that LINE-1 hypomethylation was occurred through the oxidative DNA damage on LINE-1 promoter. Based on ChIP experiments, oxidative stress disturbed histone modification patterns, especially on LINE-1 promoter. Enrichments of histone H3K4me3 and H3K18Ac at LINE-1 promoter were increased in ROS-treated cells compared to the untreated control. In addition, ROS-treated bladder cancer cells had migration and invasion capability greater than the untreated control cells. In conclusion, elevated formation of oxidative DNA lesions, overexpression of ORF1p, and alteration of chromatin modification were shown in human bladder cancer tissues. Experimentally, oxidative stress induced by ROS caused hypomethylation of LINE-1 and enrichment of active chromatin marks at LINE-1 promoter. These, at least in part, led to increased expression of LINE-1 ORF1p protein. The current findings suggest that LINE-1 reactivation by oxidative stress promotes bladder cancer progression. Therefore, downregulation of LINE-1 expression and attenuation of oxidative stress could be potential targets for bladder cancer therapy in the future.
CTL-mediated immune evasion in hiv viraemic controllers and non-controllers
Authors:   , Kaewpreedee Prathanporn 
Published: September 2023

Objective: To compare T cell response and to identify the mechanism of escape in HIV-infected volunteers.
Methods: HIV-infected volunteers (CD4>450 cells/µL) were enrolled from the anonymous clinic. Volunteers were classified into two groups based on HIV plasma viral load; viraemic controllers (VC) (VL<2000 cp/mL) and non-controllers (NC) (VL>2000 cp/mL). EDTA-whole blood was collected and determined the response against Gag p24 by IFNγ ELISpot assay. Population sequencing within the Gag p24 region was performed to identify escape mutations. Results: Demographic data [age, gender, sexual preference and the presence of protective alleles (HLA-B27, -B57 and –B58)] between VC (n=23) and NC (n=41) were no statistical significance. IFNγ-secreting cells response against Gag p24 as determining by ELISpot assay revealed that some volunteers could not respond to epitopes which are restricted by their HLA alleles (termed as non-responder). The HLA-B27-KK10 non-responders contain HLA polymorphism, HLA-B2706 which accommodates different amino acid with HLA-B2704 and HLA-B2705. The HLA-B57-TW10 and QW9, non-responders did not respond because escape mutation (T242N and T3S, respectively) was found within non-responders’ sequences. Interestingly, HLA-A11-AK11 epitope at the amino acid level was not different among responders and non-responders. Codon usages were subsequently compared and it was found that at the C-terminal position (K; Lysine) preferred different codons for translating to lysine. AAG codon is more preferable in AK11-responders while AAA codon is preferred in AK11 non-responders. In conclusion, these findings may contribute to better knowledge on HIV pathogenesis and the way to improve vaccine efficacy in the future.
Assessment of human papillomavirus detection using different methods and clinical samples
Authors:   , Nilyanimit Pornjarim 
Published: September 2023

Human papillomavirus (HPV) is the etiological agent implicated cancers include those of the cervix, vulva, vagina, penis, anus, rectum and oropharynx. Currently, the Papanicolaou (Pap) smear is the recommended for cervical cancer screening but only a small percentage of women follow this screening. Overall, this thesis aimed to develop the technique and type of specimen to detect HPV DNA in Thai population and to characterize of the HPV genotypes in Thai patients with anogenital warts. Cervical swab and urine sample of 116 women and 100 men were collected before the Pap smear test. The histological examination of women can classified into 4 group: normal cytology in 52, ASCUS in 9, LSIL in 24, and HSIL in 31. In male patients, there were 45 heterosexual males who visited hospital for health check-up and 55 were documented as homosexuals or MSM. HPV DNA was genotyping by electrochemical DNA chip and PCR sequencing showed HPV16 was the most prevalence in this study. The HPV detection rate was generally lower in urine samples compared with cervical samples. There was good agreement for detection of carcinogenic HPV from female cervical samples between the DNA chip and PCR/sequencing, with 88.8% agreement. In male urine samples, the level of agreement was higher in heterosexuals compared with homosexuals. Moreover, the 164 pair of urine and cervical specimens were tested with Genoarray assay. The result showed overall concordance percentage for HPV detection was 65.2%. In addition, the comparison of 101 self-collected vaginal and physician-collected cervical swabs of known cytology was performed by DNA chip assay. The result showed the concordance was relatively high between self-collected and physician-collected samples. The most common HPV genotype detected was HPV 51. Also, 58 cervicovaginal samples were tested for HPV genotypes using four methods in parallel: nested-PCR followed by conventional sequencing, INNO-LiPA, DNA chip, and NGS. These findings show that 7 HPV genotypes (16, 18, 31, 33, 45, 56, and 58) were identified by all four methods. Nineteen HPV genotypes were detected by NGS, but not by nested-PCR, INNO-LiPA, or DNA chip. This findings further showed the alternative method to detected HPV genotyping. Finally, the study was investigate HPV-associated anogenital warts in the Thai population and whether genotypes found are represented in the vaccine. A total of 206 anogenital swab samples were analyzed by PCR/sequencing. HPV positive was identified in 88.3% (182/206) of the samples. The majority of HPV (75.2%) were low-risk genotypes HPV6 and HPV11. This finding conclusion, this study demonstrated urine and self-collected swab is a promising technique for HPV screening. NGS may serve as an alternative for diagnostic HPV genotyping in certain situations. Moreover, quadrivalent vaccine could potentially prevent the genital warts in the Thai population.
Effects of genistein on non-alcoholic steatohepatitis (NASH) induced by high-fat high-fructose diet in bilateral ovariectomized rats
Authors:   , Pummoung Sudaporn 
Published: September 2023

To investigate the effects of genistein on estrogen deficiency with high-fat high-fructose (HFHF) diet-induced NASH rats. Female Sprague-Dawley rats (n=48) were randomly divided into ovariectomized (OVX) and non-ovariectomized (non-OVX) group. Both rat groups, further allocated into 3 subgroups; controls, rats fed with HFHF diet (NASH), and rats fed with HFHF diet plus daily 16 mg/kg BW of genistein (NASH+Gen) for 8 weeks. Serum samples were collected for liver enzymes (AST and ALT) and TNF-α level analysis. Liver tissue samples were harvested for histopathology, hepatic lipid accumulation (oil red o stained, FFA and TG level), hepatic lipid peroxidation (MDA level), hepatic inflammation (TNF-α and NF-kB) and hepatocytes apoptosis (TUNELs) examination. Protein expression of PPARγ, adiponectin, and estrogen receptor subtypes were analyzed by western blot. Rats fed with HFHF showed typical histopathology of NASH in both non-OVX and OVX groups as demonstrated by significantly increased of all histological feature scores of Brunt’s criteria when compared with controls. The most severe histopathological damage was observed in OVX rats with NASH group. Hepatic lipid accumulations (ORO, TG, and FFA) were augmented in both non-OVX and OVX rats fed with HFHF. These augmentation were significantly higher in NASH rats with OVX than non-OVX. Additionally, inflammatory markers; serum TNF-α, hepatic MDA, and NF-kB, were enhanced in diet-induced NASH with both non-OVX and OVX groups. Moreover, hepatocytes apoptosis, and hepatic PPARγ expression were increased in both non-OVX and OVX rats with NASH. Interestingly, similarly levels of hepatic MDA, FFA, PPARγ, and adiponectin were observed between control and NASH with OVX groups. These might be the influence of estrogen deficiency and suggested the impact of estrogen deficiency in NASH pathogenesis. Estrogen receptors (ERα and ERβ) expression in liver displayed the opposite pattern after induced to NASH, since, ERα increased and ERβ decreased in NASH rats of both non-OVX and OVX. NASH rats that received daily 16 mg/kg BW of genistein improved the histopathological damage in both non-OVX and OVX as exhibited significantly lowered of all histological features scores. Furthermore, genistein significantly attenuated the inflammation, lipid accumulation, oxidative stress, and hepatocyte apoptosis in both non-OVX and OVX rats with NASH. Genistein displayed the properties as PPARγ antagonist and adiponectin agonist in NASH rats. In both non-OVX and OVX rats, the expression of ERα was declined with genistein administration as compared to the NASH groups, whereas, ERβ expression was not altered. In conclusion, HFHF diet is able to develop NASH pathogenesis and could be intensified by estrogen deficiency, suggesting the protective effect of estrogen on NASH. Genistein administration may protect NASH in both non-OVX and OVX rats.
Microencapsulation of eugenol in polyelectrolyte complexes of chitosan and alginate
Authors:   , Supachettapun Chamaiporn 
Published: September 2023

Eugenol is widely used in several applications such as food activity packaging to prolong shelf life of fruits and vegetables because of its antimicrobial activity. However, eugenol is easy to decompose, leading to reduce its activity. In this work, we prepared eugenol loaded alginate-chitosan microparticles in order to solve this problem and controlled release essential oil using ionic gelation technique. The effect of concentration of eugenol (0.1-0.5 g), calcium chloride (0.5 – 8 %W/V) and surfactant (2-10% W/V) was investigated. The results showed that the optimum condition was eugenol 0.1 g, calcium chloride 2% W/V and surfactant 4% W/V. The SEM image of alginate-chitosan microparticles as ration 1:1 showed a regular distribution and spherical shape with the size of 1 mm and after loading eugenol into the beads, it was still spherical in shape. FTIR spectrum showed the interaction between carboxylic group of alginate and amine group of chitosan. According to the release profiles, it was found that release rate of eugenol loaded alginate-chitosan microparticles was higher than that of eugenol loaded alginate microparticles. In addition, strawberries were selected as a fruit model for evaluation of antimicrobial properties of eugenol loaded alginate-chitosan microparticles. The results showed that eugenol loaded alginate-chitosan microparticles could inhibit microbial in strawberries and prolong shelf-life up to 12 days. The measurements of color, lightness (L*), hue angle (h°), weight loss, and total soluble solid during storage at 0, 4, 8 and 12 days confirm that there are no significantly difference between treatment group of strawberries and control. However, the firmness of treatment group increased, which might be due to the beads could adsorb moisture, which is a major factor related to their firmness.
Functional surfactants in lubricant for microelectronic industry
Authors:   , Khongphow Chuenkamol 
Published: September 2023

The functional surfactants, as the additional additives in the standard glycol-based lubricant were studied and optimized to reduce and protect the surface of the magnetic read/write head from deposited contaminants during the lapping processes. The scanning electron microscopy (SEM) and energy-dispersive x-ray spectroscopy (EDS) results identified that the black contamination was bismuth (Bi) particles, came out from lapping plate during the lapping process due to wear. Selected surfactants exhibited the results in reduce and prevent the deposition of contaminants by forming the micelle beyond their critical micelle concentration (CMC) values. We found that both polyethoxylate alcohol (PEAL) and quaternary amines (QA) can form their micelle in glycol-based lubricant at the ratio of 1 %wt. Polyoxyethylene sorbitan monolaurate (PO) can protect the surface by its oxyethylene groups group at the 0.2 %wt in based lubricant. The characteristics and performances of modified lubricants were measured the change in magnetoresistive resistance value (%DMRR) from quasi-static test (QST). The modified lubricants had minor distribution failure of slider lower than standard lubricant. Moreover, the potentiodynamic polarization measurement results indicated that additional surfactant can also yield almost 300% better performances than the standard lubricant in term of corrosion protection during lapping processes. Thus, these surfactants can function both as the contamination protective additive and as anti-corrosion additive. These modified lubricants can also keep the lap time and other physical performances to match the fabrication requirements.
Bioactive compounds from dendrobium infundibulum
Authors:   , Ranong Salinee 
Published: September 2023

Phytochemical study of the methanol extract from Dendrobium infundibulum (Orchidaceae) led to isolation of nine pure compounds including two new compounds (dendroinfundin A and dendroinfundin B) and seven known compounds (ephemeranthol A, moscatilin, aloifol I, batatasin III, 3,3'-dihydroxy-4,5-dimethoxybibenzyl, 3,4'-dihydroxy-3',4,5-trimethoxybibenzyl and dendrosinen B). Their structures were determined from their spectroscopic data. All compounds were then examined for their lipase and alpha-glucosidase inhibitory activities. Dendrosinen B (IC50 = 295.0±37.9 µM) showed moderate inhibitory activity against lipase when compared with orlistat (IC50 = 31.4±0.6 nM). Strong anti alpha-glucosidase agents were batatasin III (IC50 = 148.8±8.4 µM) and dendrosinen B (IC50 = 213.9±2.4 µM), which were more potent than acarbose (IC50 = 809.1±22.2 µM).
The quality of complete denture, patient's satisfaction, oral health-related quality of life, and their association
Authors:   , Limpuangthip Nareudee 
Published: September 2023

The objective of this study was to determine the association of complete denture quality, patient’s satisfaction, and Oral Health-related Quality of Life (OHRQoL). A total of 126 complete denture wearers was interviewed for the primary outcome, OHRQoL, using Oral Impacts on Daily Performances (OIDP) index. Secondary outcomes were patient’s satisfaction and masticatory performance. Denture retention and stability were scored following the conventional and Chulalongkorn University (CU)-modified Kapur criteria. Esthetic-assessment criteria was evaluated from patient’s photographs. It was found that limited eating/chewing was the most common problem, and an ill-fitting denture was a primary cause of any reported problems. Unacceptable denture retention and/or stability associated with lower masticatory performance, eating dissatisfaction, and reported oral impact. CU-modified Kapur criteria revealed higher sensitivity in estimating all patient-based outcomes compared to the conventional criteria. Denture age and esthetic-assessment criteria associated with none of patient-based outcomes. In conclusion, the CU-modified Kapur criteria for complete denture retention and stability evaluation is an alternative tool in estimating masticatory ability and OHRQoL with higher sensitivity. The CU-modified Kapur criteria helps clinician in making decision whether a denture needs refabrication. In addition to professional evaluation, OHRQoL assessment should be used in evaluating a treatment need or outcome.
ความสามารถในการเข้าถึงด้านการมองเห็นในที่ทำการไปรษณีย์ของไทยสำหรับคนสายตาเลือนราง
Authors:   , ซุยยะกิจ อภิมุข 
Published: September 2023

กลุ่มคนสายตาเลือนรางมีการมองเห็นด้อยประสิทธิภาพกว่าคนสายตาปกติอย่างมาก ทำให้ขาดความสะดวกและความปลอดภัยในการทำกิจกรรมประจำวันทั้งภายในและภายนอกอาคาร งานวิจัยนี้มีวัตถุประสงค์เพื่อหารูปแบบ ขนาด และสีของลูกศรบอกทิศทางที่เห็นได้ชัดและสื่อความหมายได้ชัดเจนสำหรับผู้ที่มีการมองเห็นเลือนราง เพื่อนำไปใช้ในที่ทำการไปรษณีย์ไทย งานวิจัยได้ออกแบบลูกศร 8 แบบ ให้ผู้สังเกตที่มีสายตาปกติสวมใส่แว่นตาจำลองการมองเห็นเลือนราง 3 ชนิด ได้แก่ การมองเห็นแบบลานสายตาแคบ (NV) ที่ให้ค่าลานสายตา 10 องศา การมองเห็นแบบตามัว (BL) และการมองเห็นเลือนรางเนื่องมาจากหลอดเลือดจอประสาทตาอุดตัน (OLS) ที่ให้ค่าสายตา 0.04 ขั้นแรกให้ผู้สังเกตจำนวน 20 คนเลือกแบบลูกศรที่สังเกตได้ชัดเจนและสื่อสารบอกทิศทางได้ดีที่สุด โดยได้ลูกศรแบบที่ 1 จากนั้นนำลูกศรที่ได้มาปรับการนำเสนอด้วยสีแดง สีเขียว สีน้ำเงินและสีเหลือง ตามที่ระบุในมาตรฐานผลิตภัณฑ์อุตสาหกรรมสีและเครื่องหมายเพื่อความปลอดภัย เลขที่ มอก. 635-2554 และออกแบบให้เป็นพอสิทิฟ และเนกาทิฟ และนำไปหาขนาดที่เหมาะสมที่ทำให้คนสายตาเลือนรางทั้ง 3 แบบมองเห็นได้ชัดเจน พบว่ามีขนาด 5.5 องศาเมื่อมองห่างจากภาพลูกศร 1 เมตร จากนั้นเพิ่มจำนวนแบบโดยเพิ่มขอบเครื่องหมายลูกศรรวม 8 แบบให้ผู้สังเกตระบุทิศทางและบันทึกเวลาตอบสนอง ผลที่ได้ถูกนำไปจำลองวางบนภาพที่ถ่ายจากที่ทำการไปรษณีย์จริง ตรวจสอบระยะเวลาการตอบสนองของลูกศรเปรียบเทียบกันระหว่างในกลุ่มผู้สังเกตที่สายตามีความคมชัดน้อยกว่า 0.05 กับสายตาปกติที่สวมแว่นตาจำลอง และกลุ่มผู้สังเกตที่มีสายตาเลือนรางกับสายตาปกติที่สวมแว่นตาจำลอง ซึ่งพบว่าผู้สังเกตสายตาเลือนรางมีการตอบสนองต่อลูกศรไวกว่าผู้สังเกตสายตาปกติที่สวมแว่นตาจำลองและ ผู้สังเกตสายตามีความคมชัดน้อยกว่า 0.05 ตามลำดับ โดยผู้สังเกตสายตาเลือนรางตอบสนองต่อกลุ่มลูกศรสีน้ำเงินได้ดีกว่าลูกศรในกลุ่มสีอื่น
การแสดงออกของซีดี 146 ในรอยโรคไลเคน แพลนัสและมะเร็งช่องปากในผู้ป่วยไทย
Authors:   , ปริยะวาที สรินธร 
Published: September 2023

การศึกษาครั้งนี้มีวัตถุประสงค์เพื่อศึกษาการแสดงออกของซีดี 146 ในเยื่อเมือกช่องปากปกติ (NOM) ไลเคน แพลนัสช่องปาก (OLP) อีพิทีเลียล ดิสเพลเซียช่องปาก (OED) และมะเร็งเยื่อบุผิวเซลล์สควอมัสช่องปาก (OSCC) ในผู้ป่วยไทย โดยนำชิ้นเนื้อจากกลุ่มตัวอย่าง กลุ่มละ 20 ชิ้นมาทำการศึกษาด้วยวิธีทางอิมมูโนฮิสโตเคมี เพื่อประเมินจำนวนเคอราติโนไซต์ที่มีการแสดงออกของซีดี 146 และความเข้มในการติดสี พบว่ามีการเพิ่มขึ้นอย่างมีนัยสำคัญของค่าเฉลี่ยร้อยละของจำนวนเคอราติโนไซต์ที่มีการแสดงออกของซีดี 146 ในกลุ่ม OLP (P < 0.001) และกลุ่ม OED (P < 0.001) เมื่อเทียบกับกลุ่ม NOM โดยกลุ่ม NOM, OLP, OED มีค่าเฉลี่ยร้อยละของจำนวนเคอราติโนไซต์ที่มีการแสดงออกของซีดี 146 เท่ากับ 19.04±15.32, 59.40±24.48, 60.04±28.87 ตามลำดับ พบการลดลงอย่างมีนัยสำคัญของค่าเฉลี่ยร้อยละของจำนวนเคอราติโนไซต์ที่มีการแสดงออกของซีดี 146 ในกลุ่ม OSCC เมื่อเทียบกับกลุ่ม OED (P<0.001) โดยค่าเฉลี่ยร้อยละของจำนวนเคอราติโนไซต์ที่มีการแสดงออกของซีดี 146 ในกลุ่ม OSCC เท่ากับ 22.13±21.03 พบความแตกต่างอย่างมีนัยสำคัญของความเข้มในการติดสีระหว่างกลุ่ม NOM กับกลุ่ม OLP (P<0.05) กลุ่ม NOM กับกลุ่ม OED (P<0.05) กลุ่ม OLP กับกลุ่ม OED (P<0.05) กลุ่ม OLP กับกลุ่ม OSCC (P<0.05) และกลุ่ม OED กับกลุ่ม OSCC (P<0.05) ชิ้นเนื้อส่วนใหญ่ของกลุ่ม NOM และกลุ่ม OSCC ติดสีเข้มระดับจาง กลุ่ม OLP ติดสีเข้มปานกลาง และกลุ่ม OED ติดสีเข้ม ผลการศึกษานี้พบว่าซีดี 146 อาจมีบทบาทหลายด้านในรอยโรคข้างต้น โดยมีการแสดงออกที่เพิ่มขึ้นในไลเคน แพลนัสและอีพิทีเลียล ดิสเพลเซียช่องปาก แต่มีการแสดงออกลดลงในมะเร็งเยื่อบุผิวเซลล์สควอมัสช่องปาก ซีดี 146 จึงอาจเกี่ยวข้องกับพยาธิกำเนิดของโรคไลเคน แพลนัสช่องปาก และการสูญเสียการแสดงออกของซีดี 146 อาจเกี่ยวข้องกับการเปลี่ยนแปลงจากอีพิเลียล ดิสเพลเซียไปเป็นมะเร็งเยื่อบุผิวเซลล์สควอมัสช่องปากได้