bluuebunny/crossref_metadata_2025_split huggingface dataset:
Identification and characterization of human skeletal stem cell-like cells derived from infrapatellar fat pad
Authors: Xu Yan , Xun Jinrui , Li Zan , Lou Wenqiang , Shi Xin , Qiu Xinzhu , Zhang Tao , Yong Cao , Li Na , Hu Jianzhong , Xu Ren , Lu Hongbin 
Published: March 2023

Abstract
Background
Skeletal stem cells (SSCs) have attracted extensive attention for their crucial role in bone accrual and therapeutical values. The substantial unmet cellular need of regenerative medicine and tissue engineering calls for identification of a novel source for human SSC isolation, or even skeletal stem cell-like cells (SSCLCs).
Methods
hSSCLCs were isolated through enzyme-digestion and fluorescent-activated cell sorting (FACS) from human tissues including placenta, cord blood, Wharton’s Jelly and various adipose depots. Proportion of hSSCLCs in all those tissues were compared through flow cytometry. For adipose tissue, immunofluorescent staining was also employed to substantiate our flow results. In vitro CFU-F assay, chondrogenic and osteogenic assays were performed to assess self-renewal and multipotency for differentiation of hSSCLCs. Transcriptomic profiling of adipose-derived hSSCLCs was achieved through scRNA-seq.
Results
Here, we illustrated that adipose tissues contain a satisfying abundancy of hSSCLCs, especially infrapatellar fat pad (IPFP), but not fetal tissues. Moreover, we discovered IPFP-derived hSSCLCs display intact self-renewal and a marked elevation in chondrogenic and osteogenic differentiation. Transcriptomically comparing IPFP-hSSCLCs and dorsal adipose depot (DSAT)-derived hSSCLCs through scRNA-seq, we further demonstrated IPFP-hSSCLCs are less differentiated but more motivated in expressing transcriptomes related to chondrogenic and osteogenic differentiation.
Conclusion
Our study first identified adipose tissue as an alternative but encouraging source for isolating hSSCLCs with intact SSC properties which might be promising in treating diseases related to bone and/or cartilage defects.
Checking the rationality of a kind of Theta Functions by starting with a formula of Poisson
Authors: Louiz Akram 
Published: March 2023

Abstract
This is a mathematical example and a logical opportunity for those who doubt the summation of Poisson or the related definitions of Riemann. By using a formula of Poisson, this article makes a mathematical proof by considering that a sum is rational. We prove by contradiction that not only one infinite series is irrational but a function of real numbers defined by infinite series. Furthermore, we conclude that this function is not continuous in any point of its curve. Finally, you will find a logical explanation based on mathematical principles that may be helpful for all students of Analysis who will find a new opportunity to demonstrate.
Prediction model for seat position identification using patient profiles, bone fracture, and dislocation characteristics in a collision and non-collision motorcycle accident in Thailand
Authors: Kluakamkao Gampon , Muengtaweepongsa Sombat , Wongyikul Pakpoom , Lokeskrawee Thanin , Jantapoon Prathompong , Keelapaeng Pongsabhak 
Published: March 2023

Abstract
Motorcycle accidents accounted for the most common prevalence of Road traffic collision (RTC). Therefore, identifying the rider or passenger is crucial for ensuring fairness. However, patients who suffered from RTC frequently could not provide any information due to loss of consciousness, memory loss, or death. We aim to develop two separately multivariable prediction models based on the differences in the facture pattern and demographic data between the rider and passenger in collision with another vehicle and non-collision motorcycle accident. A total of 1,816 patients had fractures from motorcycle accidents. 1,583 and 233 were riders and passengers, respectively. After a multivariable logistic regression with stepwise backward elimination, six final predictors, including Age, sex, femur fracture, wrist and hand fracture, leg including ankle fracture, and pelvis and lumbar spine fracture, were required for the final models. The prediction models had an acceptable level of discrimination (auROCs of 0.79 and 0.77 for collision and non-collision accidents, respectively) and appeared well-calibrated. Overall, the prediction model is potentially useful as an assisting tool for identifying seat positions.
Carbon tax vs. carbon market: Which one could better help China’s coal-based electricity industry achieve carbon neutrality?
Authors: Li Yin , Song Yazhi , Li Zhongfei , Liu Tiansen 
Published: March 2023

Abstract
This paper aims to investigate which carbon pricing mechanism, carbon tax or carbon market, could better promote the carbon neutrality of China’s coal-based electricity industry that indeed exerts significant impacts on climate change and industrial prosperity. By building a stochastic electricity price model, we reveal the price shock of both carbon tax and carbon market under different carbon pricing goals. Our key empirical results are as follows. First, both carbon tax and carbon market could significantly trigger the price volatility of coal-based electricity industry, while the shock impact of carbon market on such industry’s carbon emission is more significant than that of carbon tax. Second, by both carbon pricing mechanisms, carbon emission could be reduced up to 20%, and a key premise of achieving this goal is keeping carbon price at the level of 100 yuan/ton. Third, the volatility range of electricity price, which is policy-led, could not manifest the incentive impact of economic instruments on carbon emission reduction of coal-based electricity industry. Further, policy allows the upper limit of floating electricity price up to 15%. In general, we straighten out the linkage of carbon pricing tools and coal-based electricity costs, thereby constructing a carbon pricing mechanism for coal-based electricity industry to step into China’s carbon neutrality as soon as possible.
Associations of glucose metabolism and diabetes with heart rate variability: A population-based cohort study
Authors: Yu Linling , Yang Meng , Nie Xiuquan , Zhou Min , Tan Qiyou , Ye Zi , Liu Wei , Liang Ruyi , Feng Xiaobin , Wang Bin , Chen Weihong 
Published: March 2023

Abstract
Objective
To investigate potential causal pathways and temporal relationships of glucose metabolism and diabetes with heart rate variability (HRV).
Methods
The cohort study was developed in 3858 Chinese adults. At baseline and 6 years follow-up, participants underwent HRV measurement (low frequency [LF], high frequency [HF], total power [TP], standard deviation of all normal-to-normal intervals [SDNN], and square root of the mean squared difference between adjacent normal-to-normal intervals [r-MSSD]) and determination of glucose homeostasis (fasting plasma glucose [FPG] and insulin [FPI], homeostatic model assessment for insulin resistance [HOMA-IR]). Cross-lagged panel analysis was used to examine the temporal relationships of glucose metabolism and diabetes with HRV.
Results
FPG, FPI, HOMA-IR, and diabetes were cross-sectionally negatively associated with HRV indices at baseline and follow-up (P <0.05). Cross-lagged panel analyses showed significant unidirectional paths from baseline FPG to follow-up LF (β=-0.06), and baseline diabetes to follow-up low TP group (β=0.08), low SDNN group (β=0.05), and low r-MSSD group (β=0.10) (P <0.05). No significant path coefficient from baseline HRV to follow-up impaired glucose homeostasis or diabetes was observed. The results were still statistically significant after excluding participants taking antidiabetics.
Conclusions
The findings provide strong evidence that elevated FPG and diabetes may be the causes rather than the consequences of HRV reduction over time.
Vitamin D deficiency impairs testosterone secretion and spermatogenesis in mice: the role of the transsulfuration pathway
Authors: Jamshidian-Ghalehsefidi Narges , Rabiee Farzaneh , Tavalaee Marziyeh , kiani Shaghayegh , Pouriayevali Farnaz , Razi Mazdak , Dattilio Maurizio , Nasr-Esfahani Mohammad Hossein 
Published: March 2023

AbstractVitamin D deficiency is a global health problem and has been linked to defective spermatogenesis and male infertility. In this study, we aimed to investigate the main enzymes are involved in transsulfuration pathway of 1-carbone metabolism, and spermatogenesis function. Therfore, sixteen male C57 mice were addressed to a control (standard diet) or vitamin D deficient (VDD) diet for 14 weeks. The results show that compared to the standard diet, VDD increased final body weight and reduced sperm quality, caused damage to the testicular structure, and decreased the serum levels of testosterone. In addition, serum concentrations of homocysteine, vitamin B12, and sperm oxidative stress markers increased. In testicular tissues, the CBS and CSE protein level were down-regulated whereas HO-1 was up-regulated at both mRNA and protein expression levels. Within a mice deprivation model, VDD deeply suppressed testosterone and impaired spermatogenesis with oxidative stress-mediated mechanisms. The effects of the deprivation appeared to be at least in part independent of genomic and receptor-mediated vitamin D actions and suggest a specific impairment of the alternative transsulfuration pathway.
An unequal new information priority grey forecasting air pollution urban model considering the regional socio-economic development
Authors: tu leping , Dang Yaoguo , Wang Junjie 
Published: March 2023

Abstract
The prediction of air quality has been a hot research topic in environmental issues. Due to the obvious regional characteristics of air pollution in Handan, the prediction of the Air Quality Composite Index (AQCI) in Handan was carried out considering the socio-economic factors in Handan and the surrounding cities. Firstly, the main factors affecting the AQCI of Handan were screened from the added value of primary industry, added value of industry, added value of construction industry, added value of tertiary industry, number of resident population and vehicle ownership in Handan and its five surrounding cities using a grey correlation analysis model. Then, considering the variability of development trends and information priorities among different city factors, a grey unequal new information priority multivariate convolution prediction model (UNGMC(1,N)) is proposed, and the particle swarm algorithm is used to adaptively optimize the nonlinear model parameters. The model comparison results show that the stability and prediction accuracy of the proposed UNGMC(1,N) model are significantly higher than those of other grey models and some popular air quality prediction methods. According to the research results, the air quality of Handan city is mainly influenced by the number of permanent residents in Handan city, and the air quality of Handan city is negatively correlated with the number of permanent residents in Handan city. In terms of regional pollution, the air quality of Handan city shows a positive correlation with the added value of tertiary industry in the neighboring city of Anyang. In addition, Handan's air quality will continue to improve in the future with U-shaped improvement efficiency and 2024 will be a critical year for Handan's air quality improvement efficiency.
Dietary management in pregnant Phenylketonuria (PKU) patients: comparison with protein and phenylalanine requirements in healthy pregnancies
Authors: Ennis Madeleine , Heath Annie Rosen , Branov Jennifer , Bosdet Taryn , Lehman Anna , Sirrs Sandra , Elango Rajavel 
Published: March 2023

Abstract
Background: Diet, with an emphasis on phenylalanine restriction, is recognized as an effective treatment for phenylketonuria (PKU). A similar approach is used in pregnant PKU patients to reduce teratogenic effects of elevated circulating phenylalanine. Our objective was to assess the dietary management of pregnant PKU individuals at Vancouver General Hospital, Canada, retrospectively including dietary recommendations, phenylalanine intake, patient compliance and a comparison with protein and phenylalanine requirements determined in healthy pregnancies.
Methods: We reviewed dietitian’s health records for pregnant patients monitored for PKU between 1999-2019 at the Adult Metabolic Diseases Clinic (AMDC). Data were extracted on demographics, gestational weight gain, gestational age at delivery, pregnancy outcomes, plasma analyses, reported health concerns, and dietary intake of phenylalanine, tyrosine, and protein. We present descriptive statistics of subject characteristics and clinical results. Paired sample t-test assessed for differences between prescribed phenylalanine and phenylalanine intake. Generalized additive mixed models approach was used to observe changes in phenylalanine concentration in blood spots and phenylalanine tolerance throughout the pregnancy.
Results: Eleven patients aged 20-40 years, with a total of 16 pregnancies, were included for analysis. Prescribed phenylalanine intake increased from 7.2 ± 4.3 to 16.7 ± 4.8 mg·kg-1·d-1 between early (13-19wk) and late (33-39wk) gestation. Actual phenylalanine intakes increased from 7.5.2 ± 4.3 to 17.7 ± 4.9 mg·kg-1·d-1 between these stages and were not statistically different. When compared to our previously determined mean phenylalanine requirements in healthy pregnancies during ~16wk pregnancy (15 mg·kg-1·d-1) and late (~36wk) pregnancy (21 mg·kg-1·d-1), phenylalanine intake was 50% lower at 13-19wk gestation and 16 % lower at 33-39wk gestation. Mean phenylalanine concentration in blood spots decreased as pregnancy progressed, with the mean concentration being highest preconception, and a slight increase between 8 and 12 wk.
Conclusions: Current management practices at AMDC are working well to achieve targeted metabolic control. Increase in phenylalanine requirements during early healthy pregnancy, combined with slight increase in blood spot phenylalanine in early PKU pregnancy is intriguing and suggests a need to directly determine phenylalanne needs. This natural history analysis provides reference values and management practices of pregnant PKU patients, contributing to the limited data available.
Biomechanical Comparison of Static and Dynamic Cervical Plates in terms of Bone Fusion, Tissue Degeneration, and Implant Behavior
Authors: Chung Tzu-Tsao , Hueng Dueng-Yuan , Lin Shang-Chih 
Published: March 2023

Abstract
Introduction:
Using an anterior cervical fixation device in the anterior cervical discectomy and fusion (ACDF) has evolved to various systems of static and dynamic cervical plates (SCP and DCP). Dynamic cervical plates have been divided into three categories: the rotational (DCP-R), translational (DCP-T), and hybrid (DCP-H) joints. However, little studies have been devoted to systematically investigate the biomechanical differences of dynamic cervical plates.
Materials and Methods
The biomechanical tests of load-deformation properties and failure modes between the SCP and DCP systems are implemented first by using the UHMWPE blocks as the vertebral specimens. The CT-based C2-C7 model simulates the strategies of cervical plate in ACDF surgery is developed with finite-element analyses. One intact, one SCP and two DCP systems are evaluated for their biomechanical properties of bone fusion and tissue responses.
Results
In the situation of biomechanical test, The mean values of the five ACDSP constructs are 393.6% for construct stiffness (p < 0.05) and 183.0% for the first yielding load (p < 0.05) less than those of the SCP groups, respectively. In the situation of finite-element analysis, the rigid-induced ASD is more severe for the SCP, followed by the DCP-H, and the DCP-R is the least.
Discussion and Conclusions:
Considering the degenerative degree of the adjacent segments and osteoporotic severity of the instrumented segments is necessary while using dynamic system. The mobility and stability of the rotational and translational joints are the key factors to the fusion rate and ASD progression. If the adjacent segments have been degenerative, the more flexible system can be adopted to compensate the constrained mobility of the ACDF segments. In the situation of the osteoporotic ACDF vertebrae, the stiffer system is recommended to avoid the cage subsidence.
Association between routine Cell Salvage use for Lower Segment Caesarean Section and post-operative iron infusion and hemoglobin during the Covid-19 Pandemic
Authors: Fox Tom P , Timpani Evelyn , Green Amanda , Parange Anupam , Sinha Romi , Kelly Thu-Lan , Hodyl Nicolette A , Froessler Bernd 
Published: March 2023

Abstract
Purpose
Intraoperative cell salvage is central to Patient Blood Management including for lower segment caesarean section. Prior to April 2020, we initiated intraoperative cell salvage during caesarean section based on risk assessment for hemorrhage and patient factors. As the pandemic broadened, we mandated intraoperative cell salvage to prevent peri-partum anemia and potentially reduce blood product usage. We examined the association of routine intraoperative cell salvage on maternal outcomes.
Methods
We conducted a single-center non-overlapping before-after study of obstetric patients undergoing lower segment caesarean section in the 2 months prior to change in practice (‘usual care = selective intraoperative cell salvage’, n = 203) and the 2 months following (‘mandated intraoperative cell salvage’, n = 228). Recovered blood was processed when a minimal autologous reinfusion volume of 100 ml was expected. Post-operative iron infusion and length of stay were modelled using logistic or linear regression, using inverse probability weighting to account for confounding.
Results
More emergency lower segment caesarean sections occurred in the Usual Care group. Compared to the Usual Care group, post-operative hemoglobin was higher and anemia cases fewer in the Mandated intraoperative cell salvage group. Rates of post-partum iron infusion were significantly lower in the Mandated intraoperative cell salvage group (OR = 0.31, 95% CI = 0.12 to 0.80, P = 0.016). No difference was found for length of stay.
Conclusion
Routine cell salvage provision during lower segment caesarean section was associated with a significant reduction in post-partum iron infusions, increased post-operative hemoglobin and reduced anemia prevalence.
Dengue Hemorrhagic Fever with bleeding and fluid overload in a patient with active Lupus Nephritis: a case report of diagnostic and therapeutic challenges
Authors: Vidanapathirana Manudi , Atukorale Inoshi 
Published: March 2023

Abstract
Background: Diagnosis and management of dengue hemorrhagic fever (DHF) can be challenging in the presence of confounding comorbidities. Important confounders are conditions that alter hematological parameters and intra/extra vascular fluid distribution. We report the case of a patient with active lupus nephritis, who developed DHF with subsequent bleeding and fluid overload. This is the first case report to highlight a unique set of diagnostic and therapeutic challenges in DHF in this context.
Case presentation: A seventeen-year-old girl with lupus nephritis class IV developed a renal flare of lupus and subsequently developed DHF with bleeding per vaginum. Due to acute kidney injury, she was managed with a restrictive fluid approach during the ascending limb with blood transfusion when required, and close monitoring for hemodynamic instability. During the descending limb, hourly input was transiently increased due to a rise in hematocrit. This precipitated nephrogenic pulmonary edema, which was managed with mechanical ventilation and continuous renal replacement therapy.
Conclusions: This patient posed two diagnostic challenges: diagnosis of dengue in a patient with lupus-related bicytopenia, and diagnosis of dengue leakage in a patient with nephrotic syndrome-related ascites. There were three therapeutic difficulties: deciding the fluid quota for DHF in the presence of renal impairment, and balancing the risks and benefits of steroids and anticoagulation in lupus nephritis with dengue. As decisions in such instances are patient-specific, sharing of individual experiences will help guide management decisions.
Association between lean mass and adolescent idiopathic scoliosis: A meta- analysis
Authors: Xu Wei , Zang Jiajie , Wu Fan 
Published: March 2023

Abstract
Objective: The objective of this meta-analysis was to evaluate the association between lean mass and adolescent idiopathic scoliosis (AIS).
Methods: English databases CENTRAL (The Cochrane Library and the Cochrane Back Review Group Trials Register), MEDLINE, EMBASE, PubMed, Web of Science and Chinese databases CBM, CNKI, VIP, WANGFANG DATA were searched for the relevant case control studies and cross-sectional studies. Two authors selected studies and extracted data independently. Data analysis was performed by Stata15.0.
Results: Six studies were included, with a total of 1742 cases of AIS and 6309 controls. AIS group had a lower lean mass compared to control group [MD = −2.15, 95% CI (−3.26, −1.04)]. In the subgroup analysis, female AIS patients had a lower lean mass than the control group [MD = −2.27, 95% CI (−4.13, −0.41)]. The mean difference of lean mass between AIS patients and control group in studies with adults [MD = −1.07, 95% CI (−1.68, −0.46)] is much greater than studies without adults [MD = −5.53, 95% CI (−9.09, −1.56)]. There was not statistically significant in European studies[MD = −2.11, 95% CI (−4.35, 0.14)], but in Asian studies lean mass in AIS patients was lower than the control group[MD = −2.55, 95% CI (−4.49, −0.61)]. Study type, gender, age, and geography condition were thought to have no effect on the primary outcome of lean mass by subgroup analysis.
Conclusion: In the meta-analysis, AIS group had a lower lean mass compared to control group, which indicated that lean mass may be involved in the pathogenesis of AIS. But limited by the number of studies we included; the above conclusions need to be validated by more high-quality studies.
The Chain Mediating Role of Rumination and Emotional Regulation Self-Efficacy in the Effect of Social Support on Depression in Chinese College Students
Authors: Zhang Junliang , Hu Zhongzheng , Zheng Shuang , Liu Yingyu 
Published: March 2023

Abstract

Objective: The purpose of this study was to explore the relationship between social support, rumination, emotion regulation self-efficacy (ERSE) and depression in college students, focusing on the mediating effect between social support and depression in college students.
Methods: A cross-sectional study of 1433 college students in Jiangxi, China was conducted between October and November 2022. A series of mediation analyses were performed using AMOS24.0 and the PROCESS macro to examine the mediation effects.
Results: The study found that depression was significantly positively correlated with rumination, while ERSE was significantly negatively correlated with social support and ERSE. Social support has a direct negative predictive effect on depression in college students. Rumination and ERSE play independent mediating and chain mediating roles in the mechanism of social support affecting college students' depression, with indirect effect values of 0.11, 0.02 and 0.01, respectively.
Conclusions: To alleviate and prevent depression in college students, emphasis should be placed on rumination and ERSE in addition to the influence of social support.