bluuebunny/crossref_metadata_2025_split huggingface dataset:
Stability mechanism and control technology of large inclined working face roof in large-scale caving area
Authors: Ma Shoulong , Wang Yueping , Wang Haibo , Gao Linsheng 
Published: February 2023

Abstract
Based on the compound roof in the 211 112 working face of mine no. 2 in the Xinji area and various newly emerging geological conditions such as crack development in coal seams and major slopes, the roof of the severely sloped working face was studied focusing on the stress, strain and fracture features, along with the mechanism of rib spalling in the coal wall and the grouting strengthening technology. According to the analysis, the pre-loaded pressure on the working face tended to be redistributed with the development of the coal roof caving zone, which resulted in new stress concentration in the coal wall before the caving zone. Compared with the stress before rib spalling, stress decreased with the increased depth of rib spalling, which caused a slightly lower risk of rib spalling. Based on the analysis, an inorganic grouting reinforcement with a water-solid ratio of 3:1 was adopted to meet the needs of filling-body strength, which reduced operation time and increased economic benefits. The work ensures the safe and efficient mining of the composite roof face with a large inclination angle of Xinji No. 2 Mine and offers a reference for the mining coal seams under similar conditions.
Altitudinal variations of the rate and temperature sensitivity of soil nitrogen mineralization on the Qinghai-Tibetan Plateau
Authors: Hou Xikang , Kou Dan , Hirota Mitsuru , Guo Tong , Lang Tao 
Published: February 2023

Abstract
Changes in soil nitrogen mineralization can impact nutrient availability, and further affect plant growth. It is unclear, however, how temperature elevation in alpine grassland will affect soil net N mineralization rate (Nmin) across altitudes. At six altitudes (3200–4200 m with an interval of 200 m) along a slope in Lenglong mountain in the northern Qinghai-Tibetan Plateau, we performed an in situ soil incubation experiment by using the resin-core method to assess altitudinal variations of Nmin. Meanwhile, we evaluated the effects of temperature elevation on Nmin and its temperature sensitivity (Q10) through a soil downward transplantation experiment based on three reference baseline altitudes (3800, 4000 and 4200 m). The results showed that high altitudes generally led to low values of Nmin. Structural equation modeling analysis revealed that Nmin along the altitude was mainly controlled by soil temperature. Increased temperature caused by the altitude transplantation significantly elevated Nmin for all of the three reference altitudes. The value of Q10 was 3.4 for soil samples transplanted from the reference altitude of 4200 m, which was about twice that of the lower reference altitudes of 4000 and 3800 m.
Nonequilibrium noise as a probe of pair-tunneling transport in the BCS–BEC crossover
Authors: Tajima Hiroyuki , Oue Daigo , Matsuo Mamoru , Kato Takeo 
Published: February 2023

Abstract
The detection of elementary carriers in transport phenomena is one of the most important keys to understand nontrivial properties of strongly correlated quantum matter. Here, we propose a method to identify the tunneling current carrier in strongly interacting fermions from nonequilibrium noise in the Bardeen–Cooper–Schrieffer to Bose–Einstein condensate crossover. The noise-to-current ratio, the Fano factor, can be a crucial probe for the current carrier. Bringing strongly correlated fermions into contact with a dilute reservoir produces a tunneling current in between. The associated Fano factor increases from one to two as the interaction becomes stronger, reflecting the fact that the dominant conduction channel changes from the quasiparticle tunneling to the pair tunneling.
Diffusion-Weighted Imaging of the Orbit: A Case Series and Systematic Review
Authors: Gupta Lalita , Peterson Eric L. , Williams Cody , Altman Emily , Harpole Ryan , Martin Douglas J. , Escott Edward J. , Timoney Peter J. , Prendes Mark A. 
Published: February 2023

Purpose:
To describe the findings of diffusion-weighted imaging (DWI) for a series of orbital lesions and provide a systematic review of relevant literature.

Methods:
A retrospective review of 20 patients with orbital lesions who underwent MRI with DWI at two academic institutions between 2015 and 2020 was performed. Lesion diagnosis was histopathologically confirmed except a presumed cavernous hemangioma. Echoplanar diffusion-weighted images had been acquired using 2 or 3 b values (b=0 and 1000 or b=0, 500, and 1000) at 1.5T or 3T. Lesions with significant artifacts were excluded. DWI sequences were analyzed by neuro-radiologists blinded to the diagnosis. Mean ADC values of lesions were calculated from a single region of interest. An independent two-tailed t test was used to compare categories of lesions with p < 0.05 considered significant. A systematic review of the literature was performed.

Results:
Our study included 21 lesions. ADC values were significantly lower for malignant lesions (0.628 ± 0.125 × 10−3 mm2/s) than inflammatory lesions (1.167 ± 0.381 × 10−3 mm2/s) (p < 0.001). ADC values were significantly lower for orbital lymphoma (mean 0.621 ± 0.147 × 10−3 mm2/s) than idiopathic orbital inflammation (mean 1.188 ± 0.269 × 10−3 mm2/s) with no overlap (p < 0.001).

Conclusions:
Orbital malignancies demonstrated lower ADC values, while inflammatory processes demonstrated higher ADC values, except IgG4-related disease. DWI and ADC values differentiated idiopathic orbital inflammation from orbital lymphoma. This study highlights the role of DWI in evaluating orbital pathology.
Usefulness of 18F-FDG PET/computed tomography metabolic parameters in predicting sarcopenia and prognosis of treatment-naive patients with non-small cell lung cancer
Authors: Li Dongjiang , Tan Xiaoyue , Yuan Hui , Yao Xinchao , Sun Xiaolin , He Li , Jiang Lei 
Published: February 2023

Purpose
Sarcopenia tremendously impacts the quality of life but remains debatable in prognostication in treatment-naive patients with non-small cell lung cancer (NSCLC). Hence, this study aimed to find a clinically feasible approach using 18F-FDG PET/computed tomography (CT) imaging parameters and clinical characteristics to predict sarcopenia and determine independent prognostic factors.

Methods
Clinical characteristics and 18F-FDG PET/CT metabolic parameters, including maximum standard uptake value, metabolic tumor volume, and total lesion glycolysis of primary tumor (SUVmax_P, MTV_P, and TLG_P) and combination of whole-body lesions (MTV_C and TLG_C) were collected in 344 treatment-naive patients with NSCLC. Skeletal muscle index at the third lumbar vertebra was calculated to determine sarcopenia. SUVmax of the psoas major muscle (SUVmax_M) was measured at the third lumbar vertebra as well. The diagnostic endpoint is the probability of sarcopenia, and the survival endpoints include progression-free survival (PFS) and overall survival (OS).

Results
Among 344 patients with NSCLC there were 271 patients with adenocarcinoma and 73 with squamous cell carcinoma (SCC). One hundred forty-seven patients (42.7%) were diagnosed with sarcopenia. Higher age, male, lower BMI, SCC, and lower SUVmax_M were correlated with a higher incidence of sarcopenia (P < 0.05), while age, sex and SUVmax_M were independently predictive of sarcopenia. Multivariate Cox-regression analysis revealed that BMI, advanced stage and TLG_C were independent predictors of PFS and OS, while sex was independently predictive of OS.

Conclusions
The incidence of sarcopenia increased with declining SUVmax of muscle. BMI, tumor stage, and TLG_C, but not sarcopenia, were found independently predictive of both PFS and OS.
The reporting of somatic sensory training interventions in individuals following a stroke is suboptimal: A systematic review and meta – research study
Authors: Feller Daniel , Pedri Caterina , Gozzer Paolo , Innocenti Tiziano , Trentin Francesca 
Published: February 2023

ABSTRACT

Objective
The purpose of this systematic review is to assess the adherence to the “Template for intervention description and replication” (TIDieR) checklist among all published randomized controlled trials (RCTs) of sensory retraining strategies (SRS) for patients with a history of stroke.

Methods
We investigated Medline, CENTRAL, and PEDro, without time restrictions, until 1st September 2021. Also, we searched for additional studies using the included studies' reference list.
We included only RCTs of adults with a history of stroke who aimed to improve sensation, via any type of intervention.
We used the TIDieR to assess the completeness of reporting on each intervention.

Results
We included 61 trials. None of the included studies declared to have used the TIDieR checklist to report interventions.
Overall, the median percentage of adherence to the twelve items of the TIDieR was 33% (IQR 25% - 50%). Only five of the single items were adequately described in more than 50% of the studies.
None of the RCTs reported the entirety of the core intervention components, as described in items three to nine.

Conclusions
This systematic review demonstrates that interventions in SRS trials are described below desirable standards. Without this information, clinicians and researchers cannot reliably replicate interventions.
Factors Associated with Stroke after COVID-19 Vaccination: A Statewide Analysis
Authors: Nahab Fadi , Bayakly Rana , Sexton Mary Elizabeth , Lemuel-Clarke Manet , Henriquez Laura , Rangaraju Srikant , Ido Moges 
Published: February 2023

AbstractBackgroundThe objective of our study was to evaluate baseline characteristics, COVID-19 infection and vaccine type and their association with stroke early after COVID-19 vaccination.MethodsIn a retrospective cohort study, we estimated the 21-day post vaccination incidence of stroke among COVID-19 first dose vaccine recipients. We linked the Georgia Immunization Registry with the Georgia Coverdell Acute Stroke Registry and the Georgia State Electronic Notifiable Disease Surveillance System data to assess the relative risk of stroke by vaccine type.ResultsAbout 5 million adult Georgians received at least one COVID-19 vaccine from December 1, 2020 to February 28, 2022: 54% received BNT162b2, 41% mRNA-1273 and 5% Ad26.COV2.S. Those with concurrent COVID infection within 21 days post vaccine had an increased risk of ischemic (OR=8.00, 95% CI: 4.18, 15.31) and hemorrhagic stroke (OR=5.23, 95% CI: 1.11, 24.64) with no evidence for interaction between vaccine type and concurrent COVID-19 infection. The 21-day post vaccination incidence of ischemic stroke was 8.14, 11.14, and 10.48 per 100,000 for BNT162b2, mRNA-1273 and Ad26.COV2.S recipients, respectively. After adjusting for age, race, gender, and COVID-19 infection status there was a 57% higher risk (OR=1.57, 95% CI: 1.02, 2.42) for ischemic stroke within 21 days of vaccination associated with the Ad26.COV2.S vaccine compared to BNT162b2.ConclusionsConcurrent COVID-19 infection had the strongest association with early ischemic and hemorrhagic stroke after first dose COVID-19 vaccination. The Ad26.COV2.S vaccine was associated with a higher risk of early post-vaccination ischemic stroke than BNT162b2.
Indigenous Reduction of Multi-drug Resistant Bacterial Pathogens in a Natural Uncontrolled Fermented Milk Ecosystem
Authors: Akram Almira , Khan Muhammad Nadeem , Ahmad Waqas , Khan Shabir Ahmad , Imran Muhammad 
Published: February 2023

AbstractRaw milk and its products have been questioned for microbiological safety. However, Dahi: an artisanally fermented milk product that is yet microbiologically unexplored is popularly known for therapeutic uses in public. Aiming safety and therapeutic assessment of dahi, samples of raw milk and dahi were analysed for microbiology and bacterial pathogens. The pathogens were also evaluated for antibiotic resistance. Further dahi samples were assessed for antipathogen effect. The total bacterial count of raw milk was between 3.80 × 1002to 2.97 × 1007and total fungal count was 2.50 × 1001to 4.90 × 1002whereas total bacterial and fungal count of Dahi was 3.00 × 1001to 4.05 × 1009and 2.00 × 1001to 5.30 × 1007. Bacterial population of the Dahi was found dominated by lactic acid bacteria. In milk samples,E. coliwas detected as 38%,S. aureus28%,L. monocytogenes3%,Salmonella42% andPseudomonas spp31%. In Dahi samples, their percentages were 7%, 10%, 7%, 8% and 2% respectively. The detected pathogens were found resistant against different antibiotics especially to third and fourth generations of cephalosporin and other beta-lactam drugs. When assessed invitro, the low incidence of pathogens in the Dahi samples were associated to its inhibitory effect against pathogens. Dahi samples also inhibited the growth of antibiotic resistance ATCC strains. The inhibitory activity of the Dahi is due to the changes occurs during fermentation but not corelated to the pH of the Dahi.
Two-way Dispatched function in Sonic hedgehog shedding and transfer to high-density lipoproteins
Authors: Ehring K. , Ehlers S.F. , Froese J. , Gude F. , Puschmann J. , Grobe K. 
Published: February 2023

SummaryThe Sonic hedgehog (Shh) signaling pathway controls embryonic development and tissue homeostasis after birth. This requires regulated solubilization of dual-lipidated, firmly plasma membrane-associated Shh precursors from producing cells. Although it is firmly established that the resistance-nodulation-division transporter Dispatched (Disp) drives this process, it is less clear how lipidated Shh solubilization from the plasma membrane is achieved. We have previously shown that Disp promotes proteolytic solubilization of Shh from its lipidated terminal peptide anchors. This process, termed shedding, converts tightly membrane-associated hydrophobic Shh precursors into delipidated soluble proteins. We show here that Disp-mediated Shh shedding is modulated by a serum factor that we identify as high-density lipoprotein (HDL). In addition to serving as a soluble sink for free membrane cholesterol, HDLs also accept the cholesterol-modified Shh peptide from Disp. The cholesteroylated Shh peptide is necessary and sufficient for Disp-mediated transfer because artificially cholesteroylated mCherry associates with HDL in a Disp-dependent manner, whereas an N-palmitoylated Shh variant lacking C- cholesterol does not. Disp-mediated Shh transfer to HDL is completed by proteolytic processing of the palmitoylated N-terminal membrane anchor. In contrast to dual processed soluble Shh with moderate bioactivity, HDL-associated N-processed Shh is highly bioactive. We propose that the purpose of generating different soluble forms of Shh from the dual lipidated precursor is to tune cellular responses in a tissue-type and time-specific manner.
Mecp2knock-out astrocytes affect synaptogenesis by IL-6 dependent mechanisms
Authors: Albizzati E. , Florio E. , Breccia M. , Cabasino C. , Pozzi D. , Boda E. , Battaglia C. , De Palma C. , De Quattro C. , Landsberger N. , Frasca A. 
Published: February 2023

AbstractSynaptic abnormalities represent a hallmark for several neurological diseases and clarification of the underlying mechanisms constitutes a crucial step towards the development of therapeutic strategies. Rett syndrome (RTT) is a rare neurodevelopmental disorder, mainly affecting females, caused by heterozygous mutations in the X-linked Methyl-CpG-Binding Protein 2 (MECP2) gene, leading to a deep derangement of synaptic connectivity. Although initial studies have supported the exclusive involvement of neurons, recent data have highlighted the pivotal contribution of astrocytes in RTT pathogenesis through non-cell autonomous mechanisms. Since astrocytes regulate synaptogenesis by releasing multiple molecules, we investigated the influence of soluble factors secreted byMecp2KO astrocytes on synaptic density. We found thatMecp2deficiency in astrocytes negatively affects their ability to support synapse formation by releasing synaptotoxic molecules, among which we identified interleukin-6 (IL-6). Notably, aberrant IL-6 expression exclusively emerges from a dysfunctional astrocyte-neuron crosstalk, and blocking IL-6 activity prevents synaptic alterations.
Eye movements decrease during effortful speech listening
Authors: Cui M. Eric , Herrmann Björn 
Published: February 2023

AbstractPupillometry is the most used objective tool to assess listening effort but has several disadvantages. The current study explores a new, objective way to assess listening effort through eye movements. Building on cognitive and neurophysiological work, we examine the hypothesis that eye movements decrease when speech listening becomes challenging. In three experiments with human participants from both sexes, we demonstrate, consistent with this hypothesis, that fixation duration increases and spatial gaze dispersion decreases with increasing speech masking. Eye movements decreased during effortful speech listening for different visual scenes (free viewing; object tracking) and speech materials (simple sentences; naturalistic stories). In contrast, pupillometry was insensitive to speech masking during story listening, highlighting the challenges with pupillometric measures for the assessments of listening effort in naturalistic speech-listening paradigms. Our results reveal a critical link between eye movements and cognitive load, and provide the foundation for a novel measure of listening effort applicable in a wide range of contexts.Significance statementAssessment of listening effort is critical for early diagnosis of age-related hearing loss. Pupillometry is most used but has several disadvantages. The current study explores a new, objective way to assess listening effort through eye movements. We examine the hypothesis that eye movements decrease when speech listening becomes effortful. We demonstrate, consistent with this hypothesis, that fixation duration increases and gaze dispersion decreases with increasing speech masking. Eye movements decreased during effortful speech listening for different visual scenes (free viewing; object tracking) and speech materials (sentences; naturalistic stories). Our results reveal a critical link between eye movements and cognitive load, and provide the foundation for a novel measure of listening effort applicable in a wide range of contexts.
Differences in density: taxonomic but not functional diversity in seaweed microbiomes affected by an earthquake
Authors: Pearman William S. , Morales Sergio E. , Vaux Felix , Gemmell Neil J. , Fraser Ceridwen I. 
Published: February 2023

AbstractHost-associated microbial communities can make important contributions to host health, and are shaped by a range of different factors ranging from host condition, environmental conditions, and other microbes. Disentangling the roles of these factors can be particularly difficult as many variables are correlated. Here, we leveraged earthquake-induced changes in host density to identify the influence of host density on microbiome composition. A large (7.8 magnitude) earthquake in New Zealand in 2016 led to widespread coastal uplift of up to ~6m, sufficient to locally extirpate some intertidal kelp populations. These uplifted populations are slowly recovering, but intertidal kelps remain at much lower densities than at nearby, less uplifted sites. By comparing the microbiome of the low and high density sites using 16S amplicon sequencing, we observed that low density populations had higher beta-diversity than high density populations with regards to taxonomic variability, while no beta-diversity differences were observed between functional categories. Using phylogenetic and taxonomic turnover approaches, we determined that dispersal limitation shapes low density populations to a greater extent, while homogeneous selection shapes high density populations to a greater extent. Our findings shed light on microbiome assembly processes, particularly highlighting that large-scale disturbances that affect host density can dramatically influence microbiome structure.
Individual recombinant repeats of MUC16 display variable binding to CA125 antibodies
Authors: Wang Chien-Wei , Hanson Eliza K. , Minkoff Lisa , Whelan Rebecca J. 
Published: February 2023

AbstractBACKGROUNDDespite its importance in the clinical management of ovarian cancer, the CA125 biomarker—located on the mucin protein MUC16—is still not completely understood. Questions remain about MUC16’s function and structure, specifically the identity and location of the CA125 epitopes.OBJECTIVEThe goal of this study was to characterize the interaction of individual recombinant repeats from the tandem repeat domain of MUC16 with antibodies used in the clinical CA125 II test.METHODSUsingE. coliexpression, we isolated nine repeats from the putative antigenic domain of CA125. Amino acid composition of recombinant repeats was confirmed by high-resolution mass spectrometry. We characterized the binding of four antibodies—OC125, M11, “OC125-like,” and “M11-like”—to nine recombinant repeats using Western blotting, indirect enzyme-linked immunosorbent assay (ELISA), and localized surface plasmon resonance (SPR) spectroscopy.RESULTSEach recombinant repeat was recognized by a different combination of CA125 antibodies. OC125 and “OC125-like” antibodies did not bind the same set of recombinant repeats, nor did M11 and “M11-like” antibodies.CONCLUSIONSCharacterization of the interactions between MUC16 recombinant repeats and CA125 antibodies will contribute to ongoing efforts to identify the CA125 epitopes and improve our understanding of this important biomarker.
Sulfate limitation increases specific plasmid DNA yield and productivity inE. colifed-batch processes
Authors: Gotsmy Mathias , Strobl Florian , Weiß Florian , Gruber Petra , Kraus Barbara , Mairhofer Juergen , Zanghellini Jürgen 
Published: February 2023

ABSTRACTPlasmid DNA (pDNA) is a key biotechnological product whose importance became apparent in the last years due to its role as a raw material in the messenger ribonucleic acid (mRNA) vaccine manufacturing process. In pharmaceutical production processes, cells need to grow in the defined medium in order to guarantee the highest standards of quality and repeatability. However, often these requirements result in low product titer, productivity, and yield.In this study, we used constraint-based metabolic modeling to optimize the average volumetric productivity of pDNA production in a fed-batch process. We identified a set of 13 nutrients in the growth medium that are essential for cell growth but not for pDNA replication. When these nutrients are depleted in the medium, cell growth is stalled and pDNA production is increased, raising the specific and volumetric yield and productivity. To exploit this effect we designed a three-stage process (1. batch, 2. fed-batch with cell growth, 3. fed-batch without cell growth). The transition between stage 2 and 3 is induced by sulfate starvation. Its onset can be easily controlled via the initial concentration of sulfate in the medium.We validated the decoupling behavior of sulfate and assessed pDNA quality attributes (supercoiled pDNA content) inE. coliwith lab-scale bioreactor cultivations. The results showed an increase in supercoiled pDNA to biomass yield by 33 % and an increase of supercoiled pDNA volumetric productivity by 13 % upon limitation of sulfate.In conclusion, even for routinely manufactured biotechnological products such as pDNA, simple changes in the growth medium can significantly improve the yield and quality.HighlightsGenome-scale metabolic models predict growth decoupling strategies.Sulfate limitation decouples cell growth from pDNA production.Sulfate limitation increases the specific supercoiled pDNA yield by 33 % and the volumetric productivity by 13 %.We propose that sulfate limitation improves the biosynthesis of over 25 % of naturally secreted products inE. coli.Abstract Figure