bluuebunny/crossref_metadata_2025_split huggingface dataset:
Simulation of daily soft multifocal contact lenses using SimVis Gekko: from in-vitro and computational characterization to clinical validation
Authors: Esteban-Ibañez Eduardo , Montagud-Martínez Diego , Sawides Lucie , Zaytouny Amal , de Castro Alberto , Sisó-Fuertes Irene , Barcala Xoana , Piñero David P. , Furlan Walter D. , Dorronsoro Carlos , Gambra Enrique 
Published: May 2023

Abstract
Multifocal contact lenses (MCLs) are one of the solutions to correct presbyopia, but their adoption is not widespread due to factors such as discomfort, lack of awareness, a lengthy fitting process and/or unexpected visual performance. To address this situation, visual simulators can be used to aid in refining the adaptation process. This study aims to obtain accurate simulations for a novel visual simulator (SimVis Gekko; 2EyesVision, Spain) of various daily commercial soft MCL designs from four different manufacturers: Dailies Total1 (Alcon), MyDay (Coopervision), 1Day Acuvue Moist (Johnson & Johnson) and Biotrue ONEday (Bausch + Lomb). In-vitro characterization of these MCLSs -for several distance powers and additions- was obtained using a NIMO TR-1504 instrument (Lambda-X, Belgium). From the averaged relative power profiles across distance powers, phase maps were reconstructed and Through-Focus Visual Strehl (TFVS) metric was calculated for each MCL design considering different optical diameters. The SimVis Gekko simulation corresponding to each MCL design and pupil diameter was obtained computationally and validated on bench. Finally, the MCL simulations were clinically validated in a pilot study involving presbyopic patients with different refractive errors and additions. The results of the clinical validation show a good agreement between the SimVis Gekko simulations and the real MCLs (for all the designs) when measuring through-focus visual acuity (TFVA) curves and VA at three real distances. All MCL designs showed a partial correlation (rxy,z) higher than 0.90 and a Root Mean Square Error (RMSE) below 0.07 logMAR between the TFVA of SimVis Gekko simulations and Real MCLs across subjects. The validity of the simulation approach using SimVis Gekko and using experimental measurements obtained with Nimo TR-1504 was therefore confirmed by the clinical results obtained in this study, what opens the possibility of using this visual simulator to assist and speed up the fitting process of MCLs.
Epidemiology of apical periodontitis in a representative rural population: Association with diabetes mellitus, arterial hypertension, and smoking - a cross-sectional study
Authors: Camponogara Jeanni Gonçalves , Ferreira Ticiane de Góes Mário , Pelissari Thayná Regina , Anversa Alessandro Meneghetti , Moreira Carlos Heitor Cunha , Bier Carlos Alexandre Souza 
Published: May 2023

Abstract
ObjectiveTo determine whether the systemic diseases diabetes mellitus (DM) and arterial hypertension (AH), and smoking are associated with apical periodontitis (AP) in a representative rural population.
Materials and methods Cross-sectional study using a representative sample of individuals obtained from a population-based epidemiological survey carried out in the rural area of the city of Rosário do Sul, RS, Brazil, from March 2015 to May 2016. Data were collected through structured questionnaires and clinical examination. Binary logistic regression analysis was performed to identify variables independently associated with AP.
ResultsOf the 584 individuals included in the study, 353 (60.4%) had AP. The presence of AP was independently associated with age (odds ratio [OR] = 1.018, 95% confidence interval [CI]: 1.001-1.035, p = 0.041), active or former smoker (OR = 2.109, 95% CI: 1.256-3.540, p = 0.005) and DM or prediabetes (OR = 1.696, 95% CI: 1.164-2.471, p = 0.006)
ConclusionThe study identified significant associations between AP and clinical risk factors. However, it is essential to acknowledge the study's limitations and recommend conducting longitudinal studies to confirm these associations and explore other potential variables involved in the development of AP.
Clinical Relevance The study emphasized the significance of comprehending and managing risk factors in preventing and treating AP.
Adolescent mental health is affected by neighborhood mental health conditions at the beginning of adolescence
Authors: Ueno Yuki , Okumura Yasuko , Endo Kaori , Yamasaki Syudo , Ando Shuntaro , Kasai Kiyoto , Nishida Atsushi , Koike Shinsuke 
Published: May 2023

Abstract
Neighborhood characteristics are thought to be associated with adolescent health and behavior. However, there have been very few population-based surveys using data from individual responses living in the same school district area. Tokyo TEEN Cohort data, collected from three Tokyo municipalities, were used to examine the association between psychological symptoms in school districts during early adolescence (age 10) and later psycho-behavioral conditions in adolescents (ages 12 and 14). Hierarchical linear models showed that school district-level depressive symptoms, psychological well-being, and prosocial behaviors at age 10 were associated with the corresponding individual-level indices at age 12, after controlling for demographic characteristics and baseline individual scores as covariates. Furthermore, School district-level behavioral problems at age 10 were associated with individual-level indices at ages 12 and 14. These results suggest that school district environments in which young adolescents grow up influence adolescents’ development and well-being.
Transcriptomics reveals a core transcriptional network of K-type cytoplasmic male sterility microspore abortion in wheat (Triticum aestivum L.)
Authors: Wu Baolin , Xia Yu , Zhang Gaisheng , Wang Yongqing , Wang Junwei , Ma Shoucai , Song Yulong , Yang Zhiquan , Ma Lingjian , Niu Na 
Published: May 2023

Abstract
Background
Cytoplasmic male sterility (CMS) plays a crucial role in hybrid production. K-type CMS, a cytoplasmic male sterile line of wheat with the ctyoplasms of Aegilops kotschyi, is widely used due to its excellent characteristics of agronomic performance, easy maintenance and easy restoration. However, the mechanism of its pollen abortion is not yet clear.
Results
In this study, wheat K-type CMS MS(KOTS)-90-110 (MS line) and it’s fertile near-isogenic line MR(KOTS)-90-110 (MR line) were investigated. Cytological analysis indicated that the anthers of MS line microspore nucleus failed to divide normally into two sperm nucleus and lacked starch in mature pollen grains, and the key abortive period was the uninucleate stage to dinuclear stage. Then, we compared the transcriptome of MS line and MR line anthers at these two stages. 11360 and 5182 DEGs were identified between the MS and MR lines in the early uninucleate and binucleate stages, respectively. Based on GO enrichment and KEGG pathways analysis, it was evident that significant transcriptomic differences were “plant hormone signal transduction”, “MAPK signaling pathway” and “spliceosome”. We identified 17 and 10 DEGs associated with the IAA and ABA signal transduction pathways, respectively. DEGs related to IAA signal transduction pathway were downregulated in the early uninucleate stage of MS line. The expression level of DEGs related to ABA pathway was significantly upregulated in MS line at the binucleate stage compared to MR line. The determination of plant hormone content and qRT-PCR further confirmed that hormone imbalance in MS lines. Meanwhile, 1 and 2 DEGs involved in ABA and Ethylene metabolism were also identified in the MAPK cascade pathway, respectively; the significant up regulation of spliceosome related genes in MS line may be another important factor leading to pollen abortion.
Conclusions
We proposed a transcriptome-mediated pollen abortion network for K-type CMS in wheat. The main idea is hormone imbalance may be the primary factor, MAPK cascade pathway and AS may also play important regulatory roles in this process. These findings provided intriguing insights for the molecular mechanism of microspore abortion in K-type CMS, and also give useful clues to identify the crucial genes of CMS in wheat.
Oral health behaviors for young low-income urban children during the COVID-19 pandemic: a mixed methods analysis
Authors: Martin Molly A , Sundararajan Vyshiali , Ochoa Nadia , Dziak John , Berbaum Michael , Lee Helen H , Avenetti David M , Zhang Tong , Sandoval Anna , Torres Javier , Wu Andy 
Published: May 2023

Abstract
This research assessed oral health behaviors changes in urban families with young children during the stay-at-home period of the COVID-19 pandemic. Survey data on oral health behaviors were collected in homes at three points over one year before COVID-19, and then via phone during COVID-19. Multivariate logistic regression was used to model tooth brushing frequency. A subset of parents completed in-depth interviews via video/phone that expanded on oral health and COVID-19. Key informant interviews via video/phone were also conducted with leadership from 20 clinics and social service agencies. Interview data were transcribed and coded, and themes were extracted. COVID-19 data collection went from Nov 2020 – August 2021. Of the 387 parents invited, 254 completed surveys in English or Spanish (65.6%) during COVID-19. Fifteen key informant (25 participants) and 21 parent interviews were conducted. The mean child age was approximately 4.3 years. Children identified as mainly Hispanic (57%) and Black race (38%). Parents reported increased child tooth brushing frequency during the pandemic. Parent interviews highlighted significant changes in family routines that impacted oral health behaviors and eating patterns, suggesting less optimal brushing and nutrition. This was linked to changed home routines and social presentability. Key informants described major disruptions in their oral health services and significant family fear and stress. In conclusion, the stay-at-home period of the COVID-19 pandemic was a time of extreme routine change and stress for families. Oral health interventions that target family routines and social presentability are important for families during times of extreme crisis.
Co-removing methane and carbon dioxide from the atmosphere: Process concepts and analysis
Authors: Nazir Shareq Mohd , Sirig Devesh Sathya Sri Sairam , Goel Aditya 
Published: May 2023

Abstract
Methane is the second largest contributor to global warming after CO2, and it is hard to abate due to its low concentration in the emission sources and in the atmosphere. However, removing methane from the atmosphere will accelerate achieving net-zero targets, since its global warming potential is 28 over a 100-year period. This work presents first-of-its-kind process concepts for co-removal of methane and CO2 that combines the catalytic conversion of methane step (thermal/photo-catalytic) with CO2 capture. Proposed processes have been analyzed for streams with lean methane concentrations, which are non-fossil emissions originating in the agricultural sector or natural emissions from wetlands. If the proposed processes can overcome challenges in catalyst/material design to convert methane at low concentrations, they have the potential to remove more than 40% of anthropogenic and natural methane emissions from the atmosphere at a lower energy penalty than the state-of-the-art technologies for direct air capture of CO2.
Comparative analysis of reconstructed ancestral proteins with their extant counterparts suggests primitive life had an alkaline habitat
Authors: Fujikawa Takayuki , Sasamoto Takahiro , Zhao Fangzheng , Yamagishi Akihiko , Akanuma Satoshi 
Published: May 2023

Abstract
To understand the origin and early evolution of life it is crucial to establish characteristics of the primordial environment that facilitated the emergence and evolution of life. One importantenvironmental factor is the pH of the primordial environment. Here, we assessed the pH-dependent thermal stabilities of previously reconstructed ancestral nucleoside diphosphate kinases and ribosomal protein uS8s. The selected proteins were likely to be present in ancient organisms such as the last common ancestor of bacteria and that of archaea. We also assessed the thermal stability of homologous proteins from extant acidophilic, neutralophilic, and alkaliphilic microorganisms as a function of pH. Our results indicate that the reconstructed ancestral proteins are more akin to those of extant alkaliphilic bacteria, which display greater stability under alkaline conditions. These findings suggest that the common ancestors of bacterial and archaeal species thrived in an alkaline environment. Moreover, we demonstrate the reconstruction method employed in this study is a valuable technique for generating alkali-tolerant proteins that can be used in a variety of biotechnological and environmental applications.
A dynamic online nomogram for predicting death in hospital after aneurysmal subarachnoid hemorrhage
Authors: Li Tian , zhuang Dongzhou , Xiao Yong , Chen Xiaoxuan , Zhong Yuan , Ou Xurong , Peng Hui , Sheng Jiangtao , Chen Weiqiang , Wang Shousen 
Published: May 2023

Abstract
Background This study aimed to confirm the validity of the multiplication of neutrophils and monocytes (MNM) and a novel dynamic nomogram for predicting in-hospital death in patients with aneurysmal subarachnoid hemorrhage (aSAH).Methods An analysis of 986 patients who underwent endovascular coiling for aSAH was conducted retrospectively. Univariate and multivariate logistic regression analysis identified independent risk factors associated with in-hospital death. A dynamic nomogram of in-hospital deaths was introduced in the development cohort and published online in the form of a simple calculator. Receiver operating characteristic analysis, calibration analysis, and decision curve analysis were performed to estimate the nomogram for predicting the in-hospital death from the external validation cohort.Results 72/687 patients (10.5%) in the development cohort and 31/299 (10.4%) in the validation cohort died. Based on univariate and multivariate regression analyses, MNM was associated with in-hospital death. A novel nomogram showed excellent predictive power for in-hospital death in the development cohort. The calibration curves indicated that the nomogram has reliable consistency and calibration degree. The net benefit of the nomogram was better than that of the basic model with threshold probabilities between 10% and 90%. In the validation cohort, the MNM and nomogram also exhibited good predictive values for in-hospital death.Conclusions MNM is a new indicator used to predict in-hospital death of patients with aSAH. A dynamic nomogram is an effective tool for predicting in-hospital death in aSAH patients.
CENPA and IQGAP3 Identified as Ideal Early-Stage Hepatocellular Carcinoma Markers
Authors: shen junwei , Hu Jing , Zhuang Huaping , Deng Jiahe , Yang Xi , Xie Fandi , Liu Dingsheng , Li Yanfei 
Published: May 2023

Abstract
Backgrounds:
Hepatocellular carcinoma is a type of liver cancer that has a high mortality rate and there is an urgently need to improve the prognosis and survival rates for patients with this disease. However, reliable prognostic or therapeutic markers for HCC is limited.
Results
Here, we conducted a gene expression analysis to identify candidate biomarkers that are highly expressed in tumor tissues but barely detectable in normal tissues. Among the 33 candidate genes identified, 14 were found to be significantly associated with patient survival. Further analysis revealed two highly sensitive and specific biomarkers, CENPA and IQGAP3, which were virtually undetectable in normal liver tissue but highly detectable in liver tumor tissue at both the protein and mRNA levels. Notably, they were all regulated by DNA methylation. Based on these findings, we established a new clinical classification method for hepatocellular carcinoma, which accurately stratifies patients into early or advanced stage tumors.
Conclusions
Our study not only identifies novel molecular markers for hepatocellular carcinoma, but also provides a promising approach for improving the diagnosis and treatment of this devastating disease.
Feasibility of the set-up for the different approaches in robotic head and neck surgery with the Versius Surgical System
Authors: Granell Jose , Ramirez-Rosa Alex , Fernandez-Rastrilla Isabel , Granados-Sitges Juan , Caballero Pablo , Granell Lucia , Sanchez-Camon Isabel , Mendez-Benegassi Ivan , Gutierrez-Fonseca Raimundo 
Published: May 2023

Abstract
Introduction. The Versius Surgical System is a next generation soft-tissue robot with instrument and endoscope arms split into individual modules. Despite its similarities with previous systems, the basic changes in the design raise concerns relating to the feasibility of the set-up for the different approaches in robotic head and neck surgery procedures. Materials and Methods. We used a complete unit with a surgeon´s console and four modules on a training mannequin to depict the different configurations in the operating room. We tested transoral robotic surgery and the four basic configurations for the remote access to the neck: transoral/transvestibular, retroauricular, axillary and bilateral axillo-breast approaches. Results. We obtained a high quality simulation for transoral robotic surgery, as well as for the usual remote access approaches, except for the axillary approach. We were able to obtain an optimal distribution of the modules around the surgical table and an adequate configuration of the joints allowing the instruments to reach their targets. Conclusion. The Versius Surgical System might be an alternative device for robotic procedures in Head and Neck Surgery, although this needs to be proved in a clinical setting.
The Bacterial Gq Signal Transduction Inhibitor Fr900359 Impairs Soil-associated and Plant Pathogenic Nematodes
Authors: Hanke Wiebke , Alenfelder Judith , Liu Jun , Gutbrod Philipp , Kehraus Stefan , Crüsemann Max , Dörmann Peter , Kostenis Evi , Scholz Monika , König Gabriele M. 
Published: May 2023

Abstract
The cyclic depsipeptide FR900359 (FR) is derived from the soil bacterium Chromobacterium vaccinii and known to bind Gq proteins of mammals and insects, thereby abolishing the signal transduction of their Gq protein-coupled receptors, a process that leads to severe physiological consequences. Due to their highly conserved structure, Gq family of proteins are a superior ecological target for FR producing organisms, resulting in a defense towards a broad range of harmful organisms. Here, we focus on the question whether bacteria like C. vaccinii are important factors in soil in that their secondary metabolites impair, e.g., plant harming organisms like nematodes. We prove that the Gq inhibitor FR is produced under soil-like conditions. Furthermore, FR inhibits heterologously expressed Gαq proteins of the nematodes Caenorhabditis elegans and Heterodera schachtii in the micromolar range. Additionally, in vivo experiments with C. elegans and the plant parasitic cyst nematode H. schachtii demonstrated that FR reduces locomotion of C. elegans and H. schachtii. Finally, egg-laying of C. elegans and hatching of juvenile stage 2 of H. schachtii from its cysts is inhibited by FR, suggesting that FR might reduce nematode dispersion and proliferation. This study supports the idea that C. vaccinii and its excreted metabolome in the soil might contribute to an ecological equilibrium, maintaining and establishing the successful growth of plants.
Multiomics reveals the ameliorating effect and underlying mechanism of polygonatum sibiricum rhizome water extract on HFD-induced mouse obesity
Authors: Wang Qi , Ou Xiaobin , Li Boping , Han Yapeng , Yang Yan , Gao Kai , Liu Zhengkun , Liu Haiqing , Li Dongbo , Liu Xiuli , Xu Zaoxu , Xiang Xuesong , Chen Juanjuan 
Published: May 2023

Abstract
Background Polysaccharides and ethanol extract from Polygonatum sibiricum rhizome were reported to improve high-fat diet (HFD)-fed mouse obesity. However, the effect and potential mechanism of Polygonatum sibiricum rhizome water extract (PSRwe) on HFD-induced obesity mice remains unclear. The present study was sought to comprehensively elucidate that. An obese mouse model was established by feeding HFD and PSRwe were intragastrically administered once a day for 40 days. Changes in body weight, gut microbiota and their metabolites, plasma and liver metabolomics, colonic and liver transcriptomics were explored. The compounds in PSRwe were also examined.Results Body weight was significantly reduced after two weeks of PSRwe administration. Meanwhile, PSRwe treatment has significantly recovered the richness and diversity of gut microbiota in HFD-caused obesity mice. Specifically, beneficial species including Akkermansia muciniphila and functional pathways including fatty acids biosythesis and elongation, pentose phosphate pathway, glycolysis and reductive TCA cycle were significantly enriched in PSRwe group compared with oebsity. RNA-seq revealed that the function of the up-regulated DEGs in liver of PSRwe mice were mainly concentrated on lipid and fatty acid oxidation and metabolism, while the down-regulated DEGs were mainly focused on sterols and sterol biosynthesis and metabolism. In colon, however, the function of up-regulated DEGs were primarily JAK-STAT/PI3K-Akt signaling pathway, regulation of GTPase activity, and response to cation while the down-regulated DEGs were centered on glycolysis, fructose/mannose/pyruvate metabolism, fat digestion and absorption, and PPAR signaling pathway. Metabolomics analysis indicated that betaine, an effective component from PSR water extracts, has showed higher levels in both liver and plasma of PSRwe-treated mice, indicating that it was significantly associated with obesity. In addition, correlation analysis showed that the significantly different species enriched in PSRwe group were negatively correlated with colonic DEGs related on PPAR signaling pathway, glycolysis etc. but positively correlated with JAK-STAT signaling pathway etc. Intriguingly, common metabolites in plasma and liver were negatively correlated with liver DEGs related on steroids and sterols biosynthesis and metabolism but positively correlated with fatty acid metabolism.Conclusions Collectively, our study demonstrated that PSRwe could significantly alleviate HFD-induced mouse obesity via either directly affect lipid metabolism through effective betaine or by changing gut microbiota and their metabolites to alter gene expression associated with fatty acid metabolism in liver and colon, suggesting PSRwe might be a promising therapeutic candidate for obesity in clinical.