bluuebunny/crossref_metadata_2025_split huggingface dataset:
Analisis Kesulitan Siswa Dalam Menyelesaikan Soal Matematika Materi Penyajian Data Pada Siswa Kelas VII SMP Negeri Wederok
Authors: Nahak Maria Eviana Telik , Nahak Selestina , Mone Ferdinandus 
Published: September 2023

Penelitian ini dilakukan bertujuan untuk mengetahui kesulitan dan penyebab terjadinya kesulitan yang dialami siswa dalam menyelesaikan soal penyajian data. Jenis penelitian adalah kualitatif deskriptif. Subjek dalam penelitian ini yaitu 3 orang siswa kelas VII B SMP Negeri Wederok yang mengalami kesulitan rendah, sedang dan tinggi. Teknik pengumpulan data yaitu tes dan wawancara. Berdasarkan hasil analisis data diperoleh bahwa kesulitan-kesulitan yang dialami siswa yaitu: 1) kesulitan dalam memahami konsep sebesar 50% yang dapat dilihat dari kesulitan siswa menyajikan kembalidata ke dalam bentuk diagram batang, 2) kesulitan dalam menerapkan prinsip sebesar 60% yang dapat dilihat dari kesulitan siswa dalam menerapkan prinsip pada penjumlahan siswa salah dalam perhitungan, 3) kesulitan dalam menyelesaikan masalah verbal sebesar 75%, yang dapat dilihat dari kesulitan membuat model matematika sehingga siswa tidak bisa menyelesaikan soal. Faktor penyebab siswa mengalami kesulitan yang paling banyak berasal dari faktor internal diantaranya: 1) kemampuan mengingat rendah, 2) kebiasaan belajar yang rendah, 3) jarang mengulang materi yang disampaikan guru, 4) cara belajar yang kurang baik, 5) kurang perhatian saat pembelajaran dan minat belajar rendah dan 6) kurang terampil dalam perhitungan.
Eksplorasi Etnomatematika Pada Alat Musik Tihar Dan Permainan Tradisional Biu Di Desa Lakanmau Kabupaten Belu
Authors: Bitin Meilandus Bertus , Amsikan Stanislaus , Ahzan Zulkaidah Nur 
Published: September 2023

Penelitian ini bertujuan untuk mengeksplorasi konsep-konsep matematika yang terdapat pada alat musik tihar dan permainan tradisional biu di Desa Lakanmau Kabupaten Belu. Jenis penelitian yang digunakan adalah penelitian kualitatif dengan pendekatan etnografi. Metode pengumpulan data menggunakan studi lapangan, yaitu dengan melakukan observasi, wawancara, dukumentasi pada narasumber yang sama. Instrumen penelitian yang digunakan terdiri atas instrumen utama dan instrumen bantu/pendukung. Teknik analisis data dalam penelitian ini merujuk pada rancangan Spradley yaitu analisis domain, analisis taksonomi, analisis komponensial, dan analisis tema budaya. Hasil penelitian pada alat musik tihar dan permainan tradisional biu di Desa Lakanmau terdapat konsep matematika yaitu konsep geometri dan peluang, serta nilai-nilai budaya dan manfaat dari alat musik tihar dan permainan tradisional biu yang dapat digunakan untuk membantu perkembangan pengetahuan dan keterampilan sosial. Oleh karena itu, peneliti menyarankan kepada para guru dan masyarakat dapat memanfaatkan hasil eksplorasi etnomatematika pada alat musik tihar dan permainn tradisional biu di Desa Lakanmau ke dalam pembelajaran matematika berbasis kontekstual. Masyarakat perlu menjaga dan melestarikan alat musik dan permainan tradisional anak sebagai kekhasan budaya agar tetap lestari dan tidak punah seiring perkembangan zaman, dan bagi peneliti selanjutnya agar dapat memanfaatkan hasil penelitian berupa etnomatematika pada alat musik tihar dan permainan tradisioanal biu dan menggali lebih dalam lagi konsep matematika yang terdapat pada budaya lain baik di dalam maupun di luar masyarakat Desa Lakanmau.
Molecular T2 asthma phenotypes are stable but heterogeneous: the usefulness of periostin for endotyping
Authors: Bobolea Irina , Guillén-Vera Daniela , De las Cuevas-Moreno Natividad , García-Granero Diego Blanco , Loli-Ausejo David , Melero-Moreno Carlos 
Published: September 2023

BackgroundThe stability of molecular T2/non-T2 phenotypes remains uncertain. The objectives of this study were to assess the stability of these phenotypes and the correlation between serum periostin and asthma T2 phenotypes and endotypes.MethodsDemographics, clinical data, and blood samples were collected. Patients diagnosed with moderate-to-severe asthma were classified into T2 or non-T2 according to previously defined thresholds of blood eosinophilia and serum total IgE levels. Asthma endotype was also determined. After at least 1 year of follow-up, the stability of T2 phenotypes and endotypes was assessed.ResultsA total of 53 patients (72% women), mean age 47 years (range 16–77), were included. In the initial and second evaluations, the T2 phenotype was found in 41.5% and 43.4% of patients and the non-T2 phenotype was found in 58.4% and 56.7%, respectively. The mean [standard deviation (SD), range] serum periostin level was 52.7 (26.2, 22.6–129.7) ng/mL in patients with T2 phenotype, and 39.3 (25.6, 7.7–104.) ng/mL in non-T2 patients (P = 0.063). Periostin levels correlated to endotypes (P = 0.001): 45.7 (27.9) ng/mL in allergic asthma (n = 16 patients), 64.7 (24.9) in aspirin-exacerbated respiratory disease (n = 14), 59.0 (27.6) ng/mL in late-onset eosinophilic asthma (n = 4), and 28.3 (13.3) ng/mL in non-eosinophilic asthma (n = 18).ConclusionsT2 and non-T2 asthma phenotypes assessed by accessible methods in daily practice are stable over time yet widely heterogeneous. Serum periostin does not discriminate between T2 and non-T2 phenotypes. Nevertheless, its correlation to asthma endotypes may contribute to guide therapies targeting T2 cytokines in a more personalized approach.
Dipyridamole and vascular healing following stent implantation
Authors: Simard Trevor , Jung Richard , Di Santo Pietro , Labinaz Alisha , Short Spencer , Motazedian Pouya , Dhaliwal Shan , Sarma Dhruv , Rasheed Adil , Ramirez F. Daniel , Froeschl Michael , Labinaz Marino , Holmes David R. , Alkhouli Mohamad , Hibbert Benjamin 
Published: September 2023

IntroductionPatients undergoing coronary stent implantation incur a 2% annual rate of adverse events, largely driven by in-stent restenosis (ISR) due to neointimal (NI) tissue proliferation, a process in which smooth muscle cell (SMC) biology may play a central role. Dipyridamole (DP) is an approved therapeutic agent with data supporting improved vascular patency rates. Pre-clinical data supports that DP may enact its vasculoprotective effects via adenosine receptor-A2B (ADOR-A2B). We sought to evaluate the efficacy of DP to mitigate ISR in a pre-clinical rabbit stent model.Methods &amp; Results24 New Zealand White Rabbits were divided into two cohorts—non-atherosclerosis and atherosclerosis (n = 12/cohort, 6 male and 6 female). Following stent implantation, rabbits were randomized 1:1 to control or oral dipyridamole therapy for 6 weeks followed by optical coherence tomography (OCT) and histology assessment of NI burden and stent strut healing. Compared to control, DP demonstrated a 16.6% relative reduction in NI volume (14.7 ± 0.8% vs. 12.5 ± 0.4%, p = 0.03) and a 36.2% relative increase in optimally healed stent struts (37.8 ± 2.8% vs. 54.6 ± 2.5%, p &lt; 0.0001). Atherosclerosis demonstrated attenuated effect with no difference in NI burden (15.2 ± 1.0% vs. 16.9 ± 0.8%, p = 0.22) and only a 14.2% relative increase in strut healing (68.3 ± 4.1% vs. 78.7 ± 2.5%, p = 0.02). DP treated rabbits had a 44.6% (p = 0.045) relative reduction in NI SMC content. In vitro assessment of DP and coronary artery SMCs yielded dose-dependent reduction in SMC migration and proliferation. Selective small molecule antagonism of ADOR-A2B abrogated the effects of DP on SMC proliferation. DP modulated SMC phenotypic switching with ADOR-A2B siRNA knockdown supporting its role in the observed effects.ConclusionDipyridamole reduces NI proliferation and improves stent healing in a preclinical model of stent implantation with conventional antiplatelets. Atherosclerosis attenuates the observed effect. Clinical trials of DP as an adjunctive agent may be warranted to evaluate for clinical efficacy in stent outcomes.
Clinical profile of congenital heart diseases detected in a tertiary hospital in China: a retrospective analysis
Authors: Song Linhong , Wang Yi , Wang Hui , Wang Gang , Ma Ning , Meng Qiang , Zhu Kunao , Hu Siqi , Zhou Gengxu , Feng Zhichun 
Published: September 2023

BackgroundCongenital heart diseases (CHDs) are conditions that involve structural problems to the heart's structure existing at birth, with an incidence of approximately 8 per 1,000 live births globally. CHD is one of the leading causes of maternal, fetal, and neonatal morbidity and mortality worldwide. The present study sought to examine the clinical profiles of CHD patients and provide important implications for therapeutic interventions.MethodsThis was a retrospective, observational, cohort study. The medical records of all CHDs patients aged between 0 and 18 years were collected from July 1, 2021 to June 30, 2022. Clinical profiles and demographic data were collected from cardiology and pediatric department registers for analysis.ResultsOf the 265 children with CHDs, 201 were diagnosed with acyanotic CHD (ACHD), while 64 children had cyanotic CHD (CCHD). Based on the eleventh revision of the International Classification of Diseases (ICD-11), “congenital anomaly of a ventricle or the ventricular septum” was the most common CHD. The most common symptom was failure to thrive, accounting for 18.5% of all CHD cases. The most frequent symptom in ACHD was murmur (93.53%) and sweating (80.60%), whereas the most common symptom in CCHD was sweating (95.31%) and cyanosis (84.38%).ConclusionsThis study retrospectively analyzed CHD clinical characteristics from children receiving care at the seventh center, which forms a proper basis for appropriate clinical treatments and further studies.
An interdisciplinary approach to enhance children’s listening, learning, and wellbeing in the classroom: The Listen to Learn for Life (L3) Assessment Framework
Authors: Mealings Kiri , Miles Kelly , Parrila Rauno , Holt Rebecca , Cox Felicity , Dillon Harvey , Sharma Mridula , Demuth Katherine , Leigh Greg , McMahon Catherine , McArthur Genevieve , Buchholz Joerg M. 
Published: September 2023

IntroductionListening is the gateway to children learning in the mainstream classroom. However, modern classrooms are noisy and dynamic environments making listening challenging. It is therefore critical for researchers from speech and hearing, education, and health sciences to co-design and collaborate to realistically assess how children listen to learn in the classroom and to understand how listening can be improved to enhance children’s learning and wellbeing – an understanding which is currently lacking. Such highly interdisciplinary thinking demands a holistic classroom listening framework that can integrate a range of varied assessments and outcomes.MethodsAn extensive review of literature into classroom listening was conducted but failed to identify a suitable framework. In this hypothesis and theory article we present a new framework that we have developed – the Listen to Learn for Life (L3) Assessment Framework.ResultsThe L3 Assessment Framework holistically incorporates frameworks from health, speech and hearing sciences, and education sectors. The framework accommodates a broad range of different factors that may affect listening, allowing for researchers to choose specific factors dependent on the context of use.DiscussionSelected examples of applying the framework are provided demonstrating how to assess children’s performance during different classroom activities as well as the effectiveness of a chosen intervention. For example, the framework can be used to assess the effectiveness of a wireless remote microphone intervention during group work activities for a child with autism.ConclusionThe L3 Assessment Framework provides a theoretical basis for the future development of research and practice as applied to listening in a classroom setting.
Using proteomics and metabolomics to identify therapeutic targets for senescence mediated cancer: genetic complementarity method
Authors: Fang Xiaolu , Liu Deyang , Zhao Jianzhong , Li Xiaojia , He Ting , Liu Baishan 
Published: September 2023

BackgroundSenescence have emerged as potential factors of lung cancer risk based on findings from many studies. However, the underlying pathogenesis of lung cancer caused by senescence is not clear. In this study, we try to explain the potential pathogenesis between senescence and lung cancer through proteomics and metabonomics. And try to find new potential therapeutic targets in lung cancer patients through network mendelian randomization (MR).MethodsThe genome-wide association data of this study was mainly obtained from a meta-analysis and the Transdisciplinary Research in Cancer of the Lung Consortium (TRICL), respectively.And in this study, we mainly used genetic complementarity methods to explore the susceptibility of aging to lung cancer. Additionally, a mediation analysis was performed to explore the potential mediating role of proteomics and metabonomics, using a network MR design.ResultsGNOVA analysis revealed a shared genetic structure between HannumAge and lung cancer with a significant genetic correlation estimated at 0.141 and 0.135, respectively. MR analysis showed a relationship between HannumAge and lung cancer, regardless of smoking status. Furthermore, genetically predicted HannumAge was consistently associated with the proteins C-type lectin domain family 4 member D (CLEC4D) and Retinoic acid receptor responder protein 1 (RARR-1), indicating their potential role as mediators in the causal pathway.ConclusionHannumAge acceleration may increase the risk of lung cancer, some of which may be mediated by CLEC4D and RARR-1, suggestion that CLEC4D and RARR-1 may serve as potential drug targets for the treatment of lung cancer.
Comprehensive analysis of mitophagy-related genes in diagnosis and heterogeneous endothelial cells in chronic rhinosinusitis: based on bulk and single-cell RNA sequencing data
Authors: Zhou Shican , Fan Kai , Lai Ju , Tan Shiwang , Zhang Zimu , Li Jingwen , Xu Xiayue , Yao Chunyan , Long BoJin , Zhao Chuanliang , Yu Shaoqing 
Published: September 2023

Background: Chronic rhinosinusitis (CRS) is a complex inflammatory disorder affecting the nasal and paranasal sinuses. Mitophagy, the process of selective mitochondrial degradation via autophagy, is crucial for maintaining cellular balance. However, the role of mitophagy in CRS is not well-studied. This research aims to examine the role of mitophagy-related genes (MRGs) in CRS, with a particular focus on the heterogeneity of endothelial cells (ECs).Methods: We employed both bulk and single-cell RNA sequencing data to investigate the role of MRGs in CRS. We compiled a combined database of 92 CRS samples and 35 healthy control samples from the Gene Expression Omnibus (GEO) database and we explored the differential expression of MRGs between them. A logistic regression model was built based on seven key genes identified through Random Forests and Support Vector Machines - Recursive Feature Elimination (SVM-RFE). Consensus cluster analysis was used to categorize CRS patients based on MRG expression patterns and weighted gene co-expression network analysis (WGCNA) was performed to find modules of highly correlated genes of the different clusters. Single-cell RNA sequencing data was utilized to analyze MRGs and EC heterogeneity in CRS.Results: Seven hub genes—SQSTM1, SRC, UBA52, MFN2, UBC, RPS27A, and ATG12—showed differential expression between two groups. A diagnostic model based on hub genes showed excellent prognostic accuracy. A strong positive correlation was found between the seven hub MRGs and resting dendritic cells, while a significant negative correlation was observed with mast cells and CD8+ T cells. CRS could be divided into two subclusters based on MRG expression patterns. WGCNA analysis identified modules of highly correlated genes of these two different subclusters. At the single-cell level, two types of venous ECs with different MRG scores were identified, suggesting their varying roles in CRS pathogenesis, especially in the non-eosinophilic CRS subtype.Conclusion: Our comprehensive study of CRS reveals the significant role of MRGs and underscores the heterogeneity of ECs. We highlighted the importance of Migration Inhibitory Factor (MIF) and TGFb pathways in mediating the effects of mitophagy, particularly the MIF. Overall, our findings enhance the understanding of mitophagy in CRS, providing a foundation for future research and potential therapeutic developments.
Identification and validation of disulfidptosis-associated molecular clusters in non-alcoholic fatty liver disease
Authors: Yu Xiaoxiao , Guo Zihao , Fang Zhihao , Yang Kai , Liu Changxu , Dong Zhichao , Liu Chang 
Published: September 2023

Objective: Non-alcoholic fatty liver disease (NAFLD) is the most prevalent liver disease in the world, and its pathogenesis is not fully understood. Disulfidptosis is the most recently reported form of cell death and may be associated with NAFLD progression. Our study aimed to explore the molecular clusters associated with disulfidptosis in NAFLD and to construct a predictive model.Methods: First, we analyzed the expression profile of the disulfidptosis regulators and immune characteristics in NAFLD. Using 104 NAFLD samples, we investigated molecular clusters based on differentially expressed disulfidptosis-related genes, along with the related immune cell infiltration. Cluster-specific differentially expressed genes were then identified by using the WGCNA method. We also evaluated the performance of four machine learning models before choosing the optimal machine model for diagnosis. Nomogram, calibration curves, decision curve analysis, and external datasets were used to confirm the prediction effectiveness. Finally, the expression levels of the biomarkers were assessed in a mouse model of a high-fat diet.Results: Two differentially expressed DRGs were identified between healthy and NAFLD patients. We revealed the expression profile of DRGs in NAFLD and the correlation with 22 immune cells. In NAFLD, two clusters of molecules connected to disulfidptosis were defined. Significant immunological heterogeneity was shown by immune infiltration analysis among the various clusters. A significant amount of immunological infiltration was seen in Cluster 1. Functional analysis revealed that Cluster 1 differentially expressed genes were strongly linked to energy metabolism and immune control. The highest discriminatory performance was demonstrated by the SVM model, which had a higher area under the curve, relatively small residual and root mean square errors. Nomograms, calibration curves, and decision curve analyses were used to show how accurate the prediction of NAFLD was. Further analysis revealed that the expression of three model-related genes was significantly associated with the level of multiple immune cells. In animal experiments, the expression trends of DDO, FRK and TMEM19 were consistent with the results of bioinformatics analysis.Conclusion: This study systematically elucidated the complex relationship between disulfidptosis and NAFLD and developed a promising predictive model to assess the risk of disease in patients with disulfidptosis subtypes and NAFLD.
The Cxcr2+ subset of the S100a8+ gastric granylocytic myeloid-derived suppressor cell population (G-MDSC) regulates gastric pathology
Authors: Kao Krystal D. , Grasberger Helmut , El-Zaatari Mohamad 
Published: September 2023

IntroductionGastric myeloid-derived suppressor cells (MDSCs) are a prominent population that expands during gastric pre-neoplastic and neoplastic development in humans and mice. However, the heterogeneity of this population has circumvented the ability to study these cells or understand their functions. Aside from Schlafen-4+ (Slfn-4+) MDSCs in mouse studies, which constitute a subset of this population, limitations exist in characterizing the heterogeneity of the gastric CD11b+Ly6G+ population and targeting its different subsets. Here we identify S100a8 as a pan-specific marker for this population and utilize it to study the role of the S100a8+Cxcr2+ subset.MethodsWe profiled gastric CD11b+Ly6G+ versus CD11b+Ly6G- myeloid cells by transcriptomic and single-cell RNA sequencing. We identified S100a8 as a pan-specific marker of the gastric granulocytic MDSC (G-MDSC) population, and generated S100a8CreCxcr2flox/flox to study the effects of Cxcr2 knockdown.ResultsFollowing 6-months of Helicobacter felis infection, gastric CD11b+Ly6G+ G-MDSCs were highly enriched for the expression of S100a8, S100a9, Slfn4, Cxcr2, Irg1, Il1f9, Hcar2, Retnlg, Wfdc21, Trem1, Csf3R, Nlrp3, and Il1b. The expression of these distinct genes following 6mo H. felis infection marked heterogeneous subpopulations, but they all represented a subset of S100a8+ cells. S100a8 was identified as a pan-marker for CD11b+Ly6G+ cells arising in chronic inflammation, but not neutrophils recruited during acute gut infection. 6mo Helicobacter felis-infected S100a8CreCxcr2flox/flox mice exhibited worsened gastric metaplastic pathology than Cxcr2flox/flox mice, which was associated with dysregulated lipid metabolism and peroxidation.ConclusionS100a8 is a pan-specific marker that can be used to target gastric G-MDSC subpopulations, of which the Cxcr2+ subset regulates gastric immunopathology and associates with the regulation of lipid peroxidation.
Urine SERPINC1/ORM1 as biomarkers for early detection of lupus nephritis in MRL-lpr mice
Authors: Kim Young-Eun , Lee Eun-Ju , Kim Kyunggon , Kim Do Hoon , Jeong Mi Ryeong , Yu Jiyoung , Hong Seokchan , Lee Chang-Keun , Yoo Bin , Kim Yong-Gil 
Published: September 2023

BackgroundTo evaluate the usefulness of urine SERPINC1 and ORM1 as biomarkers for early detection of lupus nephritis (LN).MethodsUsing proteomics, we screened for potential urine biomarkers that differentiate LN from systemic lupus erythematosus (SLE) patients without nephritis. In addition, urine levels of target biomarkers were measured by ELISA in 13- and 23-week-old MRL-lpr (murine model for LN) and MRL/MpJ mice. Histological analysis was also performed on the kidneys of 23-week-old mice.ResultsUrine SERPINC1 and ORM1 were elevated in SLE patients with newly diagnosed LN compared with SLE patients without LN (SERPINC1, AUC=.892, P&lt;.001; ORM1, AUC=.886, P&lt;.001). Levels of urine SERPINC1 and ORM1 were also significantly higher in MRL-lpr mice than in MRL/MpJ mice at 13 and 23 weeks (SERPINC1: p&lt;.01 and p&lt;.001 at 13 and 23 weeks, respectively; ORM1: p&lt;.01 at 13 and 23 weeks). In contrast, a significant difference in urine albumin between the two groups was only observed at 23 weeks (p&lt;.001) not at 13 weeks (p=.83). Regarding the kidney pathology of MPL-lpr mice, urine ORM1 and urine albumin, but not urine SERPINC1, were positively correlated with the activity index (ORM1, rho =.879, p&lt;.001; albumin, rho =.807, p=.003) and chronicity index (ORM1, rho =.947, p&lt;.001; albumin, rho =.869, p&lt;.001).ConclusionWe propose that urine SERPINC1 and ORM1 are novel biomarkers for early LN.