bluuebunny/crossref_metadata_2025_split huggingface dataset:
Integrated bioinformatic analysis and cell line experiments reveal the significant role of the novel immune checkpoint TIGIT in kidney renal clear cell carcinoma
Authors: Xia Qi-Dong , Li Bo , Sun Jian-Xuan , Liu Chen-Qian , Xu Jin-Zhou , An Ye , Xu Meng-Yao , Zhang Si-Han , Zhong Xing-Yu , Zeng Na , Ma Si-Yang , He Hao-Dong , Zhang Yu-Cong , Guan Wei , Li Heng , Wang Shao-Gang 
Published: March 2023

BackgroundT cell immunoglobulin and ITIM domain (TIGIT) is a widely concerned immune checkpoint, which plays an essential role in immunosuppression and immune evasion. However, the role of TIGIT in normal organ tissues and renal clear cell carcinoma is unclear. We aim to identify the critical role of TIGIT in renal clear cell carcinoma and find potential targeted TIGIT drugs.Materials and methodsData retrieved from the GTEX database and TCGA database was used to investigate the expression of TIGIT in normal whole-body tissues and abnormal pan-cancer, then the transcriptome atlas of patients with kidney renal clear cell carcinoma (KIRC) in the TCGA database were applied to distinguish the TIGIT related features, including differential expression status, prognostic value, immune infiltration, co-expression, and drug response of sunitinib an anti-PD1/CTLA4 immunotherapy in KIRC. Furthermore, we constructed a gene-drug network to discover a potential drug targeting TIGIT and verified it by performing molecular docking. Finally, we conducted real-time polymerase chain reaction (PCR) and assays for Transwell migration and CCK-8 to explore the potential roles of TIGIT.ResultsTIGIT showed a moderate expression in normal kidney tissues and was confirmed as an essential prognostic factor that was significantly higher expressed in KIRC tissues, and high expression of TIGIT is associated with poor OS, PFS, and DSS in KIRC. Also, the expression of TIGIT was closely associated with the pathological characteristics of the tumor, high expression of TIGIT in KIRC was observed with several critical functions or pathways such as apoptosis, BCR signaling, TCR signaling et al. Moreover, the expression of TIGIT showed a strong positive correlation with infiltration of CD8+ T cells and Tregs and a positive correlation with the drug sensitivity of sunitinib simultaneously. Further Tide ips score analysis and submap analysis reveal that patients with high TIGIT expression significantly show a better response to anti-PD1 immunotherapy. Following this, we discovered Selumetinib and PD0325901 as potential drugs targeting TIGIT and verified the interaction between these two drugs and TIGIT protein by molecular docking. Finally, we verified the essential role of TIGIT in the proliferation and migration functions by using KIRC cell lines.ConclusionsTIGIT plays an essential role in tumorigenesis and progression in KIRC. High expression of TIGIT results in poor survival of KIRC and high drug sensitivity to sunitinib. Besides, Selumetinib and PD0325901 may be potential drugs targeting TIGIT, and combined therapy of anti-TIGIT and other treatments show great potential in treating KIRC.
Co-detection of respiratory syncytial virus with other respiratory viruses across all age groups before and during the COVID-19 pandemic
Authors: Hayek Haya , Amarin Justin Z. , Qwaider Yasmeen Z. , Khanfar Asim , Stopczynski Tess , Schmitz Jonathan , Chappell James D. , Wrenn Jesse O. , Spieker Andrew J. , Halasa Natasha B. , Howard Leigh M. 
Published: March 2023

BackgroundPatterns of respiratory syncytial virus (RSV) co-detection with other viruses may have been disrupted during the coronavirus disease 2019 (COVID-19) pandemic, but the clinical impact of viral co-detections with RSV is not well-established. We aimed to explore the frequency and clinical outcomes associated with RSV single detection and co-detection before and during the pandemic.MethodsWe conducted a single-center retrospective cohort study of all children and adults with respiratory samples tested using a respiratory pathogen panel (RPP; 01/01/2018–11/30/2022), a provider-ordered polymerase chain reaction–based assay that detects respiratory pathogens. We stratified our cohort into age groups: 0–4, 5–17, 18–64, and ≥65 years old. Among RSV-positive samples, we compared the proportion of samples with single RSV detection before and during the pandemic and the patterns of specific viral co-detections. We compared the odds of hospitalization, oxygen use, intensive care unit admission, and intubation between individuals with RSV single detection and those with co-detection.ResultsAmong 57,940 samples collected during the study period, 3,986 (6.9%) were RSV-positive. RSV was co-detected with at least one other virus in 1,231/3,158 (39.0%), 104/348 (29.9%), 49/312 (15.7%), and 21/168 (12.5%) of samples from individuals 0–4, 5–17, 18–64, and ≥65 years old, respectively. The relative frequencies of RSV single detection and co-detection were comparable before and during the pandemic except in children 0–4 years old, in whom single RSV detections were more prevalent before (63.7%) than during (59.5%) the pandemic (p=0.021). In children 0–4 years old, RSV co-detection was associated with lower odds of hospitalization compared to single RSV detection, and RSV co-detection with parainfluenza viruses or human rhinovirus/enterovirus was associated with significantly lower odds of hospitalization, while RSV/SARS-CoV-2 co-detection was associated with higher odds of ICU admission. In adults ≥65 years old, RSV co-detection was associated with lower odds of oxygen use.ConclusionThe proportion of RSV co-detection did not appreciably vary before and during the pandemic, except in young children, though the combinations of co-detected viruses did vary. Our findings suggest that the clinical impact of RSV co-detection with other viruses may be age-associated and virus-specific.
GENETICS OF STUTTERING
Authors: Pomohaibo V. , Berezan O. , Petrushov A. 
Published: March 2023

Stuttering is characterized by frequent repetition or lengthening of sounds, syllables or words, or by frequent hesitations or pauses that disrupt the rhythmic flow of speech. It may be accompanied by movements of the face and/or other parts of the body that coincide in time with the repetitions, prolongations, or pauses in speech flow. The prevalence of stuttering ranges from 0.3% to 5.6%. Usually for 80%-90% of patients stuttering starts gradually when children are 2-7 years old. Longitudinal research shows that 65% -85% of stuttering children recover. The rate of heritability of stuttering is about 70%, indicating that almost one third of the disorder is caused by environmental factors. Currently four stuttering genes have been identified that are involved in intracellular metabolism: GNPTAB, GNPTG, NAGPA and AP4E1. The disorder is caused by single nucleotide synonymous or missense mutations in the exons of these genes. Different people affected by stuttering can carry different sets of mutant variants ‒ from one to several. The effect of the identified genes on neurobiological processes in the human brain and on the development of stuttering remains unknown. So far it has only been discovered that stuttering occurs due to lysosomal dysfunction, which adversely affects the organization of neurofilaments of speech neural networks. The contribution of identified genes to stuttering cumulatively is only 20%. There were identified also 9 candidate stuttering genes (FADS2, PLXNA4, CTNNA3, ARNT2, EYA2, PCSK5, SLC24A3, FMN1 and ADARB2) and 7 regions of 6 chromosomes (2p, 3p, 3q, 12q, 14q, 15q and 16q) reliably linked to this disorder.
Further successful genetic studies of stuttering are needed to improve our understanding and treatment of this long-enigmatic disorder.
SORPTION AND X-RAY FLUORESCENCE DETERMINATION OF MICRO QUANTITIES OF SOME TOXIC METALS IN NATURAL OBJECTS AFTER THEIR PRECONCENTRATION ON MODIFIED SILICA GEL
Authors: Kychkyruk O. , Kusiak N. , Yanovska E. 
Published: March 2023

A method for X-ray fluorescence determination of trace amounts of Pb(II), Cd(II) and Hg(II) ions (up to 250 μg/ml) directly in the sorbent phase after their extraction from solutions in a dynamic sorption mode has been developed. Merck silica gel (particle d 0.1-0.2 mm, specific surface 428.61 m2/g), chemically modified with 4-(2-pyridylazo)-resorcinol functional groups in one step, was used as a sorbent. An energy dispersive X-ray fluorescence analyzer with a semiconductor detector "ElvaX" ("Elvatex", Kyiv) was used in the study.
It was found that the combined analysis of micro quantities of Pb, Cd and Hg by sorption-x-ray fluorescence method after their preconcentration on silica gel with chemically fixed surfactant requires the use of calibration curves for each metal, taking into account the presence of other ions in solutions.
The developed by us method of sorption-x-ray fluorescence determination of micro quantities of Pb(II), Cd(II) and Hg(II) after their extraction and preconcentration on silica gel with chemically fixed 4-(2-pyridylazo)-resorcinol can be effectively used for the analysis of natural and man-made objects of complex chemical composition.
INVASION POTENTIAL OF DAREVSKIA ARMENIACA (MEHELY, 1909) IN UKRAINE: ASSESSMENT BASED ON GIS MODELING
Authors: Harbar O. , Romaniuk R. , Dotsenko I. 
Published: March 2023

The article is devoted to the actual problem of determining the invasive potential of the invader species, the Armenian rock lizard Darevskia armeniaca. A population of introduced parthenogenetic rock lizards Darevskia armeniaca has existed in the canyon of the Teteriv River (Zhytomyr region, Ukraine) for about 60 years. Their presence in the herpetofauna of Ukraine is the result of a scientific experiment aimed at studying the processes of acclimatization and the existence of a local population of clones in a limited area, the possibilities of hybridogenesis of parthenogenetic and bisexual species and other issues of population genetics.
The invasive potential of rock lizards in this area was studied by the method of bioclimatic modeling of the ecological niche. The material was based on data from the Global Biodiversity Information System (GBIF.org (03 November 2021) GBIFOccurrenceDownload. To model the ecological niche, 19 bioclimatic variables, average monthly data on solar radiation intensity for the modern period (1970-2000), data on altitude from the WorldClim 2.1 database were used. Most of the significant parameters of the ecological niche for the introduced population are in the lower pessimum zone. The model built based on the entire collection of points of registration of the species shows that the territory where the species was introduced in Ukraine is one of the least suitable. Nevertheless, the population of introduced parthenogenetic rock lizards is now stable, thriving, numerous and slightly expanding its range by inhabiting new territories. Obviously, favorable conditions for the existence of D. armeniaca here are determined not by macroclimatic factors, but by microclimatic ones.
The analysis of the model built on the basis of points of registration of the species in the introduced population shows that under existing climatic conditions, wide expansion of the species in this region of Ukraine is not possible, although a small expansion of its range is quite likely. There is also no reason to predict the transition of rock lizards to the number of invasive species.
WATER QUALITY ASSESSMENT OF PONDS OF THE CENTRAL POLISSIA BY THE STRUCTURAL AND FUNCTIONAL INDICATORS OF PHYTOPLANKTON
Authors: Kravtsova O. , Sheliuk Y. 
Published: March 2023

Reservoirs formed in the process of human activity are a new type of reservoir and an integral element of landscapes. Studies of these water bodies make it possible to establish the mechanisms of formation and functioning of phytoplankton in artificially created ecosystems. Studies were conducted in 2018-2020 on phytoplankton assemblages in ponds of the Central Polissia area in Ukraine. The work aimed to evaluate the water quality of the Central Polissia ponds according to structural and functional indicators of phytoplankton development. Differences in phytoplankton abundance, biomass, and structure, dominant complexes of species were assessed in the studied ponds. 103 species of algae, represented by 105 intraspecific taxa, including those containing the nomenclature type species, from 7 divisions, 11 classes, 20 orders, 31 families, and 60 genera were found in the studied ponds. The occurrence of 9 types of algae, which were not previously found in the territory of Ukrainian Polyassia. Chlorophyta, Euglenozoa, and Miozoa were the structure- forming divisions in the phytoplankton biomass in the studied ponds. The dominant species complex of the ponds’ phytoplankton (according to abundance and biomass) was formed by 5–18 species, which accounted for 33–62% of the species richness of the water bodies. Shannon’s diversity index indicated the predominance of the polydominant structure of phytoplankton in most ponds.
Phytoplankton assemblages of the studied ponds in central Polissia were characterized by high biodiversity, differentiated structure, and differences in dominant species, which highlight the critical role of artificial water bodies in biodiversity. This study provides necessary information for understanding the changes in the phytoplankton community caused by anthropogenic impacts on artificial water bodies.
FEATURES OF THE ORGANIZATION OF THE ADMINISTRATIVE- TERRITORIAL ORGANIZATION OF THE EASTERN PODILS REGION
Authors: Gudzevich A. 
Published: March 2023

The article examines the historical and geographical aspects of the experience organization of the administrative-territorial system of the southeastern part of Podilsk Transnistria, which still does not have a synthetic expression of this problem, in order to identify the possibilities of its application to improve functioning in conditions of decentralization. In particular, attention is focused on the establishment of transformation processes of the administrative-territorial system at the sub- regional level of the national territorial structure, taking into account its historical and geographical features throughout the entire time period, in unison with the formation of Ukraine (from ancient times to the present). The research is based on the complex use of general scientific and special scientific methods, which made it possible to cover all aspects of the scientific problem. On the basis of the basic territorial-temporal dimension, the diversity of the administrative-territorial organization of the subregional level is characterized, which is the result of the influence of various factors (historical, geographical, military-political, ethno-cultural) and reflects its main forms in the conditions of statehood (public self-government, the Vichy of Kyiv Rus, Galicia -Volyn Principality, Hetmanship, UNR, Ukraine), occupation regimes (Golden Horde, Lithuanian Principality, Polish and Romanian Kingdoms, Commonwealth of Independent States, Ottoman, Russian and German (Third Reich) Empires, Soviet Union). The study of the realities of the transformation of the administrative- territorial system of the southeastern part of Podilskyi Transnistria over a period of more than a thousand years allows us to holistically trace the peculiarities of the creation and functioning of local self-government at the sub-regional meso level, which is valuable in view of the decentralization of executive authorities and the state's strategic European integration course. The results of the research can be used in further historical-geographical studies of the administrative- territorial system of Transnistria and neighboring regions, in particular, to solve urgent problems of the territorial organization of the district-level administrative unit; for the preparation of lecture courses on general or thematic regional studies, primarily geographical and historical.
THE DEPARTMENT OF GEODESY AND CARTOGRAPHY, FACULTY OF GEOGRAPHY, TARAS SHEVCHENKO NATIONAL UNIVERSITY OF KYIV: HISTORY, MODERNITY, PROSPECTS
Authors: Oreshchenko А. 
Published: March 2023

There is considered the activity of geodesy and cartography department of geographical faculty of Taras Shevchenko National University of Kyiv from time of its institution to present time. Chronologically 3 main topics exposed: educational and scientific functioning, structural and organization instants and personnel policy of department.
To mark the periods in development of the department we took the structure of competences which were to have formed in students during theirs training. There we considered the correspondence level of educational programs to business demands too. It was proposed the structure of the educational program which is focused on perspective economy inquiries (2020-2030’s) and the wording of which is the result of a separate unfinished research.
Besides the subdivisions role in educational and scientific achievements we also disclosed the personnel policy and organizational work of the heads of the department. There are separately stated the history of scientific subdivision and pointed out on the causes of its reorganization. In the other hand we discussed the influence of external factors onto educational process, in particular computing machinery appearance, gaining of national independence and change of state economy model, conversion of cartographic production to digital technology, implementation of the Bologna education system.
In the final part of the article we recounted the demands for education department functioning in actual conditions of high cost of study, changing of profile specialties and academic competition for the best students with other universities which have the departments with similar specialization.
Cryoprotectors in food refrigeration technologies
Authors: Bogdanov V. D. , Pankina A. V. 
Published: March 2023

The purpose of the work: systematization of trends in the development of scientific research on the creation of effective cryoresistant systems for the refrigeration technology of food, including from aquatic biological resources.Results: analytical studies have shown the widespread use of cryoprotectors in cryobiology, aquaculture, the production of various food products and other industries. In the food industry, it is advisable to use natural materials that do not have toxicity and are components of food products as cryoprotectors.Novelty of the work: a promising direction is the use of products from hydrobionts of dry seafood concentrates obtained by cryotechnology as cryoprotectors in refrigeration technologies. They have high functional properties and can be used both as separate preparations and as part of composite systems.Practical significance: the results obtained are used by specialists in the development of new technologies for frozen minced fish products, which, due to the use of cryoprotectors, have higher quality indicators compared to control samples.Methods used: Analytical evaluation of modern domestic and foreign scientific works in the field of cryotechnology using cryoprotectors, systematization of scientific data and justification of directions for the creation of cryoprotective compositions for food refrigeration technology.
Studies of the migration of juvenile Pacific salmon in the Savushkina river (Paramushir Island, Northern Kuriles) in 2022
Authors: Uglova T. Yu. , Sokolov A. V. , Marchenko S. L. 
Published: March 2023

The purpose of the research is to form scientific basis of effective management of salmon fisheries in the Russian Far East.Applied methods: research work was carried out in accordance with standard methods used in fisheries research.Scientific novelty: data on qualitative and quantitative composition of juvenile Pacific salmon migrating from the Savushkina River for rearing in marine waters in May-July 2022. Based on qualitative heterogeneity of juveniles, presence of eco-temporal groups within chum salmon and coho salmon was supposed.Result: data on timing and dynamics of seaward migration of Pacific salmon juveniles, as well as their qualitative and quantitative patterns.Practical relevance: Results of the research would be used in forecasting of commercial catch of Pacific salmon in the Northern Kurils.
The Role of Light In The Architecture of Religious Buildings
Authors: Iskandar Iskandar , Zacky Zulfan , Ratika Isti Adelina , P. Agustiananda Putu Ayu , Budiman Hanif 
Published: March 2023

Light is one of the important factors in human life. Lighting especially in houses of worship is considered a very important part for symbolic and spiritual reasons. Light is the main focus in architecture, both in Islamic and Gothic architecture. The purpose of this study is to summarize the role of light in buildings of worship, especially houses of worship. This study uses a qualitative method of literature study. The results of this study indicate that the role of light in buildings of worship, especially houses of worship, is very important for the congregation, both spiritually and visually. Spiritually, the role of light can provide inner peace and also the closeness of the servant to his God. from a visual point of view the role of light can provide beauty and comfort to the room. From these results, the conclusion is that the role of light in religious buildings in the building of houses of worship is very important for worshippers, in shaping the inner kayakinan both in spiritual terms and in Visual terms
MASALAH PENDIDIKAN DAN PROFESIONALISASI SUMBER DAYA MANUSIA
Authors: Vinsensius Sumardi  
Published: March 2023

Masalah Pendidikan dan Profesionalisasi Sumber Daya Manusia. Realitas pendidikan Indonesia seakan tidak pernah berhenti dari masalah. Keluhan yang umum didengar terkait dengan mutu, relevansi dan daya saing. Tentu tidak berlebihan jika dinilai bahwa mutu pendidikan di Indonesia masih rendah bila dibandingkan dengan beberapa negara lainnya di Asia Tenggara. Belum lagi, soal relevansi dan daya saing. Terbaca bahwa tingkat penggangguran di Indonesia masih dinilai tinggi. Soal ini, tentu membutuhkan solusi. Tawaran yang dikemas dalam tulisan ini berkaitan dengan pengaturan sumber daya manusia pendidikan melalui profesionalisasi pendidik dan tenaga kependidikan (pelatihan dan pengembangan) sebagai salah satu cara untuk mengatasi masalah pendidikan.