bluuebunny/crossref_metadata_2025_split huggingface dataset:
Meaningful coproduction with clinicians: establishing a practice-based research network with physiotherapists in regional Australia
Authors: Gleadhill Connor , Williams Christopher M. , Kamper Steven J. , Bolsewicz Katarzyna , Delbridge Andrew , Mahon Benjamin , Donald Bruce , Delore Caitlin , Boettcher Craig , Renfrew David , Manvell Joshua , Dooley Katherine , Byrne Michael , Watson Toby , Makaroff Andrew , Gibbs Benedicta , Barnett Christopher , Corrigan Michael , Leyland Murray , Mullen Nicholas , Gallagher Ryan , Zelinski Samuel , Lamond Steven , Maude Travis , Davidson Simon R. E. , Robson Emma , Da Silva Priscilla Viana , Manvell Nicole 
Published: May 2023

Abstract
Background
The disconnect between research and clinical practice leads to research evidence that is often not useful for clinical practice. Practice-based research networks are collaborations between researchers and clinicians aimed at coproducing more useful research. Such networks are rare in the physiotherapy field. We aimed to describe (i) clinicians’ motivations behind, and enablers to, participating in a network, (ii) the process of network establishment and (iii) research priorities for a practice-based network of physiotherapists in the Hunter Region of New South Wales (NSW), Australia that supports research coproduction.

Methods
We describe the methods and outcomes of the three steps we used to establish the network. Step 1 involved consultation with local opinion leaders and a formative evaluation to understand clinicians’ motivations behind, and enablers to, participating in a network. Step 2 involved establishment activities to generate a founding membership group and codesign a governance model. Step 3 involved mapping clinical problems through a workshop guided by systems thinking theory with local stakeholders and prioritizing research areas.

Results
Through formative evaluation focus groups, we generated five key motivating themes and three key enablers for physiotherapists’ involvement in the network. Establishment activities led to a founding membership group (n = 29, 67% from private practice clinics), a network vision and mission statement, and a joint governance group (9/13 [70%] are private practice clinicians). Our problem-mapping and prioritization process led to three clinically relevant priority research areas with the potential for significant change in practice and patient outcomes.

Conclusions
Clinicians are motivated to break down traditional siloed research generation and collaborate with researchers to solve a wide array of issues with the delivery of care. Practice-based research networks have promise for both researchers and clinicians in the common goal of improving patient outcomes.
Utilization obstacles to hypertension services provided at comprehensive health centers: a content analysis study
Authors: Faraji-Khiavi Farzad , Ghorbani Kalkhajeh Sasan , Gholizadeh Behnam , Dindamal Behnaz 
Published: May 2023

Abstract
Introduction
Hypertensive patients' failure to refer to health centers for the management of their disease is the most fundamental public health challenge in most societies. The aim of this study was to identify the utilization obstacles to hypertension services provided at comprehensive health centers (CHCs) from the perspective of patients and the staff of health centers.

Methods
This was a qualitative study using conventional content analysis which was conducted in 2022. Participants included 15 hypertensive patients referring to CHCs and 10 staff (including personnel of CHCs and expert staff) of Ahvaz Jundishapur University of Medical Sciences, Ahvaz, southwest of Iran. Data were collected using semi-structured interviews. Interviews were analyzed using content analysis method, and coding was done manually.

Results
In total, 15 codes and 8 categories were extracted from the interviews which were organized in two main themes of "individual problems" and "systemic problems". More particularly, the main theme of “individual problems” involved attitudinal obstacles, occupational obstacles, and economic obstacles. The main theme of "systemic problems" included educational obstacles, motivational obstacles, procedural obstacles, structural obstacles, and managerial obstacles.

Conclusion
In order to address individual problems associated with the patients' failure to refer to CHCs, we need to take appropriate measures. These include using motivational interviewing techniques and utilizing the effective activity of healthcare liaisons and volunteers in CHCs to increase patients' awareness and change their negative attitudes and misconceptions. To solve systemic problems, it is imperative that effective training courses be held for health center staff.
Phylogenetic analysis of HA and NA genes of influenza A viruses in immunosuppressed inpatients in Beijing during the 2018–2020 influenza seasons
Authors: Liu Yafen , Wang Yue , Wang Yanxin , Mai Huan , Chen YuanYuan , Zhang Yifan , Ji Ying , Cong Xu , Gao Yan 
Published: May 2023

Abstract
Background
Influenza A viruses have undergone rapid evolution with virulent; however, complete and comprehensive data on gene evolution and amino acid variation of HA and NA in immunosuppressed patients was few. In this study, we analysed molecular epidemiology and evolution of influenza A viruses in immunosuppressed population, and immunocompetent population were used as controls.

Methods
Full sequences of HA and NA of A(H1N1)pdm09 and A(H3N2) were acquired through reverse transcription-polymerase chain reaction (RT-PCR). HA and NA genes were sequenced using the Sanger method and phylogenetically analysed using ClustalW 2.10 and MEGA software version 11.0.

Results
During the 2018–2020 influenza seasons, 54 immunosuppressed and 46 immunocompetent inpatients screened positive for influenza A viruses by using the quantitative real-time PCR (qRT-PCR) were enrolled. 27 immunosuppressed and 23 immunocompetent nasal swab or bronchoalveolar lavage fluid samples were randomly selected and sequenced using the Sanger method. A(H1N1)pdm09 were detected in 15 samples and the remaining 35 samples were A(H3N2) positive. By analyzing the HA and NA gene sequences of these virus strains, we found that all A(H1N1)pdm09 viruses shared high similarities to each other and the HA and NA genes of these viruses exclusively belonged to subclade 6B.1A.1. Some NA genes of A(H3N2) viruses were not in the same clade as those of A/Singapore/INFIMH-16-0019/2016 and A/Kansas/14/2017, which may have led to A(H3N2) being the dominant strain in the 2019–2020 influenza season. Both A(H1N1)pdm09 and A(H3N2) viruses showed similar evolutionary lineages patterns of HA and NA between immunosuppressed and immunocompetent patients. Compared with the vaccine strains, there were no statistically significant of HA and NA genes and amino acid sequences of influenza A viruses in immunosuppressed and immunocompetent patients. However, the oseltamivir resistance substitution of NA-H275Y and R292K have been observed in immunosuppressed patients.

Conclusions
A(H1N1)pdm09 and A(H3N2) viruses showed similar evolutionary lineages patterns of HA and NA between immunosuppressed and immunocompetent patients. Both immunocompetent and immunosuppressed patients have some key substitutions, which should be of note monitored, especially those with potential to affect the viral antigen.
Identification of deep intronic variants of PAH in phenylketonuria using full-length gene sequencing
Authors: Zhang Chuan , Yan Yousheng , Zhou Bingbo , Wang Yupei , Tian Xinyuan , Hao Shengju , Ma Panpan , Zheng Lei , Zhang Qinghua , Hui Ling , Wang Yan , Cao Zongfu , Ma Xu 
Published: May 2023

Abstract
Background
Phenylketonuria (PKU) is an autosomal recessive congenital metabolic disorder caused by PAH variants. Previously, approximately 5% of PKU patients remained undiagnosed after Sanger sequencing and multiplex ligation-dependent probe amplification. To date, increasing numbers of pathogenic deep intronic variants have been reported in more than 100 disease-associated genes.

Methods
In this study, we performed full-length sequencing of PAH to investigate the deep intronic variants in PAH of PKU patients without definite genetic diagnosis.

Results
We identified five deep intronic variants (c.1199+502A>T, c.1065+241C>A, c.706+368T>C, c.706+531>C, and c.706+608A>C). Of these, the c.1199+502A>T variant was found at high frequency and may be a hotspot PAH variant in Chinese PKU. c.706+531T>C and c.706+608A>C are two novel variants that extend the deep intronic variant spectrum of PAH.

Conclusion
Deep intronic variant pathogenicity analysis can further improve the genetic diagnosis of PKU patients. In silico prediction and minigene analysis are powerful approaches for studying the functions and effects of deep intronic variants. Targeted sequencing after full-length gene amplification is an economical and effective tool for the detection of deep intron variation in genes with small fragments.
Comparing angiotensin receptor–neprilysin inhibitors with sodium–glucose cotransporter 2 inhibitors for heart failure with diabetes mellitus
Authors: Tsai Ming-Lung , Lin Yuan , Lin Ming-Shyan , Tsai Tzu-Hsien , Yang Ning-I , Wang Chao-Yung , Hsieh I-Chang , Hung Ming-Jui , Chen Tien-Hsing 
Published: May 2023

Abstract
Background and aims
Clinical comparisons of angiotensin receptor–neprilysin inhibitors (ARNI) and sodium–glucose cotransporter 2 inhibitors (SGLT2i) treatment in patients with HFrEF and T2DM are limited. This study evaluated the clinical outcomes and treatment benefits of SGLT2i versus ARNI treatment in patients with HFrEF and T2DM in a large real-world data set.

Methods
We identified 1487 patients with HFrEF and T2DM who were undergoing ARNI or SGLT2i treatment for the first time (n = 647 and 840, respectively) between January 1, 2016, and December 31, 2021, and with clinical outcomes of CV death, hospitalization for heart failure (HHF), composite CV outcomes, or renal outcomes.

Results
The HHF risk reduction conferred by SGLT2i treatment was more significant than that conferred by ARNI treatment (37.7% vs. 30.4%; 95% confidence interval [CI] 1.06–1.41). SGLT2i use conferred significantly greater renal protection against the doubling of serum creatinine (13.1% vs. 9.3%; 95% CI 1.05–1.75), an estimated glomerular filtration rate decline of > 50% (24.9% vs. 20.0%; 95% CI 1.02–1.45), and progression to end-stage renal disease (3.1% vs. 1.5%; 95% CI 1.62–5.23). The improvements in echocardiographic parameters were comparable between the groups.

Conclusions
Compared with ARNI treatment, SGLT2i treatment was associated with a more significant HHF risk reduction and greater preservation of renal function in patients with HFrEF and T2DM. This study also supports the prioritization of SGLT2i use in these patients when patients' conditions or economic resources need to be considered.
Abnormal glycosylation in glioma: related changes in biology, biomarkers and targeted therapy
Authors: Yue Juan , Huang Roujie , Lan Zehao , Xiao Bo , Luo Zhaohui 
Published: May 2023

AbstractGlioma is a rapidly growing and aggressive primary malignant tumor of the central nervous system that can diffusely invade the brain tissue around, and the prognosis of patients is not significantly improved by traditional treatments. One of the most general posttranslational modifications of proteins is glycosylation, and the abnormal distribution of this modification in gliomas may shed light on how it affects biological behaviors of glioma cells, including proliferation, migration, and invasion, which may be produced by regulating protein function, cell—matrix and cell‒cell interactions, and affecting receptor downstream pathways. In this paper, from the perspective of regulating protein glycosylation changes and abnormal expression of glycosylation-related proteins (such as glycosyltransferases in gliomas), we summarize how glycosylation may play a crucial role in the discovery of novel biomarkers and new targeted treatment options for gliomas. Overall, the mechanistic basis of abnormal glycosylation affecting glioma progression remains to be more widely and deeply explored, which not only helps to inspire researchers to further explore related diagnostic and prognostic markers but also provides ideas for discovering effective treatment strategies and improving glioma patient survival and prognosis.
Effects of cellulase and xylanase on fermentative profile, bacterial diversity, and in vitro degradation of mixed silage of agro-residue and alfalfa
Authors: Mu Lin , Wang Qinglan , Wang Yating , Zhang Zhifei 
Published: May 2023

AbstractThe objective was to determine effects of cellulase, xylanase, and commercial fibrolytic enzymes on fermentation quality, aerobic stability, bacterial community, and in vitro degradation of mixed silages. Mixtures of alfalfa, wheat bran, and rice straw [80:15:5 on a fresh matter (FM) basis] were ensiled for 1, 3, 5, 7, 15, 30, and 45 d after treatment with: distilled water (control, C); cellulase (E); xylanase (X); or commercial fibrolytic enzymes (EX), with all enzyme preparations applied at 100 U/g FM. The 45-day silages were subjected to an in vitro degradation test. Each of the three enzyme-treated groups enriched relative abundance (RA) of Lactobacillus, Weissella, and Stenotrophomonas maltophilia, increased water soluble carbohydrate (WSC) concentrations, and extended aerobic stability over 384 h, but concurrently inhibited growth of undesirable microbes (i.e., Acinetobacter sp, Lelliottia amnigena, and Sphingomonas sp), reducing pH and concentrations of ammonia nitrogen (AN), butyric acid (BA) and propionic acid (PA). Compared to C, adding X or EX increased the RA of L. paralimentarius and L. parabrevis, enhanced accumulation of acetic acid (AA) and crude protein (CP), and reduced hemicellulose content. Furthermore, E group silage had the highest abundance of W. cibaria. In addition, EX enriched RA of Bacillus velezensis, reduced AN concentration, increased DM degradability, total VFA production, and gas production during in vitro incubation. In conclusion, addition of X or EX enhanced ensiling by enhancing concentrations of AA; however, EX was the most promising enzyme, based on reducing AN concentration and increasing DM content and DM degradability.
Graphical Abstract
Deep subduction of the Philippine Sea slab and formation of slab window beneath central Japan
Authors: Miyazaki Kazuki , Nakajima Junichi , Suenaga Nobuaki , Yoshioka Shoichi 
Published: May 2023

AbstractThe geometry of the Philippine Sea slab (PHS) subducting beneath the Japanese islands has been imaged to 400 km depth beneath the Kyushu and Chugoku regions, whereas the PHS slab geometry beneath the Hokuriku region has only been determined to ~ 140 km depth, thereby indicating a large east–west asymmetry in the slab subduction. However, geologic evidence suggests that there was symmetrical east–west seafloor spreading along the axis of the Kinan seamount chain when the Shikoku basin was an active spreading center in the PHS plate. This inconsistency suggests that the PHS slab should be present beneath the Hokuriku region. Here we perform P-wave travel-time tomography across central Japan and conduct a two-dimensional plate subduction numerical simulation that reproduces the dual subduction of the PHS and Pacific (PAC) plates to elucidate the PHS slab geometry beneath central Japan. The tomography results reveal a high-velocity anomaly at ~ 150–250 km depth that extends from Wakasa bay to Noto peninsula and a slab window beneath the Hokuriku region. The numerical simulation results suggest that the PHS slab may have torn when it collided with the PAC slab, with the once leading edge of the PHS slab now present along the upper surface of the PAC slab beneath Noto peninsula. These results indicate that the PHS slab exists at ~ 250 km depth beneath the Hokuriku region, although it has been torn owing to its collision with the PAC slab, with this tear propagating westward to form a triangular slab window beneath the Hokuriku region.
Graphical Abstract
The diabetes insulin self-management education (DIME) intervention for people with type 2 diabetes starting insulin: a pilot feasibility randomised controlled trial
Authors: Winkley Kirsty , Sorsa Taru , Tian Qingxiu , Reece Ilse , Fitzgerald Christina , Chamley Mark , Ismail Khalida , Forbes Angus , Upsher Rebecca 
Published: May 2023

Abstract

Objective
To determine the feasibility and acceptability of a diabetes insulin self-management education (DIME) group intervention for people with type 2 diabetes starting insulin.

Design
Single-centre parallel randomised pilot trial.

Setting
Primary care, South London, UK.

Subjects
Adults with type 2 diabetes, requiring insulin treatment, on maximum tolerated dose of 2 or more oral antidiabetic drugs with HbA1c > / = 7.5% (58 mmol/mol) on 2 occasions. We excluded people who were non-fluent in English; morbid obesity (BMI > / = 35 kg/m2); in employment that contraindicates insulin treatment; and those with severe depression, anxiety disorders, psychotic disorders, personality disorders, or cognitive impairment.

Methods
Participants were randomised using blocks of 2 or 4 to 3, 2-h group, face-to-face, DIME sessions or standard insulin group education sessions (control). We assessed feasibility according to consent to randomisation and attendance at intervention (DIME) and standard group insulin education sessions. Acceptability of the interventions was determined using exit interviews. We additionally measured change in self-reported insulin beliefs, diabetes distress and depressive symptoms between baseline and 6-month post-randomisation.

Results
There were 28 potentially eligible participants, of which 17 consented to randomisation, 9 were allocated to the DIME group intervention and 8 were allocated to the standard group insulin education. Three people withdrew from the study (1 from DIME and 2 from standard insulin education) before the start of the first session and did not complete baseline questionnaires. Of the remaining participants (n = 14), all DIME participants (n = 8) completed all 3 sessions, and all standard insulin education participants (n = 6) completed at least 1 standard insulin education session. The median group size was 2, the mean age of participants was 57.57 (SD 6.45) years, and 64% were female (n = 9). Exit interviews demonstrated that all participants (n = 7) found the group sessions acceptable, and thematic analysis of interview transcripts indicated social support, the content of group sessions and post-group experiences were positive, especially amongst DIME participants. There was improvement on self-report questionnaires.

Conclusions
The DIME intervention was acceptable and feasible to deliver to participants with type 2 diabetes starting insulin in South London, UK.

Trial registration
International Study Registration Clinical Trial Network (ISRCTN registration number 13339678).
Feasibility study of using mobile phone-based experience sampling to assess drug checking by opioid street drug users
Authors: Swartz James A. , Mackesy-Amiti Mary Ellen , Jimenez A. David , Robison-Taylor Lisa , Prete Elizabeth 
Published: May 2023

Abstract
Background
To date, evaluations of take-home fentanyl (and/or benzodiazepine) test strip use — the most common form of drug checking services — and potential effects on overdose risk have relied on retrospective accounts for some preceding time period, usually a week to several months. Such accounts, however, are subject to recall and memory biases. This pilot study assessed the feasibility of using experiential sampling to collect daily information in situ on drug checking and associated overdose risk reduction — the primary outcomes — among a sample of street opioid users and compared the results to retrospective reports.

Methods
We recruited 12 participants from a Chicago-based syringe services program. Participants were 18 years of age or older, reported using opioids purchased on the street 3 + times per week in the past month, and had an available Android mobile phone. A phone-based app was programmed to collect daily drug checking information and provided to each participant along with a supply of fentanyl and benzodiazepine test strips and instructions for use over 21 days. Comparable retrospective data were collected via follow-up in-person surveys at the conclusion of daily report collection.

Results
We found a reasonably high rate of daily reporting (63.5%) with participants submitting reports on 160 “person-days” out of 252 possible days. Participants submitted daily reports an average of 13 of 21 days. Reports of test strip use frequency varied between the retrospective and daily reports with a relatively higher percentage of days/time using test strips obtained from the daily reports. We also found higher proportions reporting overdose risk reduction behaviors on the daily reports compared with the retrospective reviews.

Conclusions
We believe the results support using daily experience sampling to collect information on drug checking behaviors among street drug users. Although resource intensive in comparison to retrospective reports, daily reporting potentially provides more detailed information on test strip use and its association with overdose risk reduction and, ultimately, fewer overdoses. Needed are larger trials and validation studies of daily experience sampling to identify the optimum protocol for collecting accurate information on drug checking and overdose risk reduction behavior.
Non-traditional cognitive brain network involvement in insulo-Sylvian gliomas: a case series study and clinical experience using Quicktome
Authors: Wu Zhiqiang , Hu Guanjie , Cao Bowen , Liu Xingdong , Zhang Zifeng , Dadario Nicholas B. , Shi Qinyu , Fan Xiao , Tang Yao , Cheng Zhangchun , Wang Xiefeng , Zhang Xia , Hu Xiaorong , Zhang Junxia , You Yongping 
Published: May 2023

AbstractBackgroundPatients with insulo-Sylvian gliomas continue to present with severe morbidity in cognitive functions primarily due to neurosurgeons’ lack of familiarity with non-traditional brain networks. We sought to identify the frequency of invasion and proximity of gliomas to portions of these networks.MethodsWe retrospectively analyzed data from 45 patients undergoing glioma surgery centered in the insular lobe. Tumors were categorized based on their proximity and invasiveness of non-traditional cognitive networks and traditionally eloquent structures. Diffusion tensor imaging tractography was completed by creating a personalized brain atlas using Quicktome to determine eloquent and non-eloquent networks in each patient. Additionally, we prospectively collected neuropsychological data on 7 patients to compare tumor-network involvement with change in cognition. Lastly, 2 prospective patients had their surgical plan influenced by network mapping determined by Quicktome.ResultsForty-four of 45 patients demonstrated tumor involvement (< 1 cm proximity or invasion) with components of non-traditional brain networks involved in cognition such as the salience network (SN, 60%) and the central executive network (CEN, 56%). Of the seven prospective patients, all had tumors involved with the SN, CEN (5/7, 71%), and language network (5/7, 71%). The mean scores of MMSE and MOCA before surgery were 18.71 ± 6.94 and 17.29 ± 6.26, respectively. The two cases who received preoperative planning with Quicktome had a postoperative performance that was anticipated.ConclusionsNon-traditional brain networks involved in cognition are encountered during surgical resection of insulo-Sylvian gliomas. Quicktome can improve the understanding of the presence of these networks and allow for more informed surgical decisions based on patient functional goals.