bluuebunny/crossref_metadata_2025_split huggingface dataset:
Biogeographic Patterns of Sulfur in the Vegetation of the Tibetan Plateau
Authors: Zhao Wenzong , Xiao Chunwang , Li Mingxu , Xu Li , Li Xin , Zhu Xingyu , Cheng Changjin , He Nianpeng 
Published: March 2023

AbstractSulfur (S) plays an important role in plant growth and development. However, due to climatic conditions and limited data availability, the variation and allocation of S are largely unknown at regional or global scales. In this study, we systematically evaluated the S distribution patterns and storage in vegetation on the Tibetan Plateau for the first time, based on consistent field‐measured data of 2,040 plant communities. The mean S contents of leaves, branches, trunks, and roots were 1.68, 0.40, 0.19, and 1.45 g kg−1, respectively; corresponding to S densities of 0.40 × 10−2 (9.57%), 1.18 × 10−2 (28.38%), 1.37 × 10−2 (32.90%) and 1.21 × 10−2 t hm−2 (29.15%), respectively. The mean S densities for forests (5.59 ± 0.26 × 10−2 t hm−2) were higher than that of shrublands (4.54 ± 0.51 × 10−2 t hm−2), grasslands (1.03 ± 0.07 × 10−2 t hm−2), and deserts (1.32 ± 0.28 × 10−2 t hm−2). The S density was generally lower in the northwest and higher in the southeast of the Tibetan Plateau and had divergent allocation patterns between different plant organs and vegetation types. Furthermore, we found that S in leaves and roots was more strongly influenced by environmental factors and was particularly sensitive to radiation and atmospheric pressure. Moreover, the total S storage in the vegetation of the Tibetan Plateau was estimated to be 337.32 × 104 t, with 114.32 × 104 (33.89%), 92.36 × 104 (27.38%), 128.77 × 104 (38.17%), and 1.86 × 104 t (0.55%) in the forests, shrublands, grasslands, and deserts, respectively. Our study clarified the S densities of different plant organs and ecosystems on the Tibetan Plateau and compiled 1 × 1 km vegetation S density data sets, which could help determine the key parameters for regional S cycle models in the future.
LncRNA XIST regulates breast cancer stem cells by activating proinflammatory IL-6/STAT3 signaling
Authors: Ma Yuxi , Zhu Yongyou , Shang Li , Qiu Yan , Shen Na , Wang Jonathan , Adam Tiffany , Wei Wei , Song Qingxuan , Li Jun , Wicha Max S. , Luo Ming 
Published: March 2023

AbstractAberrant expression of XIST, a long noncoding RNA (lncRNA) initiating X chromosome inactivation (XCI) in early embryogenesis, is a common feature of breast cancer (BC). However, the roles of post-XCI XIST in breast carcinogenesis remain elusive. Here we identify XIST as a key regulator of breast cancer stem cells (CSCs), which exhibit aldehyde dehydrogenase positive (ALDH+) epithelial- (E) and CD24loCD44hi mesenchymal-like (M) phenotypes. XIST is variably expressed across the spectrum of BC subtypes, and doxycycline (DOX)-inducible knockdown (KD) of XIST markedly inhibits spheroid/colony forming capacity, tumor growth and tumor-initiating potential. This phenotype is attributed to impaired E-CSC in luminal and E- and M-CSC activities in triple-negative (TN) BC. Gene expression profiling unveils that XIST KD most significantly affects cytokine-cytokine receptor interactions, leading to markedly suppressed expression of proinflammatory cytokines IL-6 and IL-8 in ALDH- bulk BC cells. Exogenous IL-6, but not IL-8, rescues the reduced sphere-forming capacity and proportion of ALDH+ E-CSCs in luminal and TN BC upon XIST KD. XIST functions as a nuclear sponge for microRNA let-7a-2-3p to activate IL-6 production from ALDH- bulk BC cells, which acts in a paracrine fashion on ALDH+ E-CSCs that display elevated cell surface IL-6 receptor (IL6R) expression. This promotes CSC self-renewal via STAT3 activation and expression of key CSC factors including c-MYC, KLF4 and SOX9. Together, this study supports a novel role of XIST by derepressing let-7 controlled paracrine IL-6 proinflammatory signaling to promote CSC self-renewal.
Engineered exosomes from different sources for cancer-targeted therapy
Authors: Zhang Menghui , Hu Shengyun , Liu Lin , Dang Pengyuan , Liu Yang , Sun Zhenqiang , Qiao Bingbing , Wang Chengzeng 
Published: March 2023

AbstractExosome is a subgroup of extracellular vesicles, which has been serving as an efficient therapeutic tool for various diseases. Engineered exosomes are the sort of exosomes modified with surface decoration and internal therapeutic molecules. After appropriate modification, engineered exosomes are able to deliver antitumor drugs to tumor sites efficiently and precisely with fewer treatment-related adverse effects. However, there still exist many challenges for the clinical translation of engineered exosomes. For instance, what sources and modification strategies could endow exosomes with the most efficient antitumor activity is still poorly understood. Additionally, how to choose appropriately engineered exosomes in different antitumor therapies is another unresolved problem. In this review, we summarized the characteristics of engineered exosomes, especially the spatial and temporal properties. Additionally, we concluded the recent advances in engineered exosomes in the cancer fields, including the sources, isolation technologies, modification strategies, and labeling and imaging methods of engineered exosomes. Furthermore, the applications of engineered exosomes in different antitumor therapies were summarized, such as photodynamic therapy, gene therapy, and immunotherapy. Consequently, the above provides the cancer researchers in this community with the latest ideas on engineered exosome modification and new direction of new drug development, which is prospective to accelerate the clinical translation of engineered exosomes for cancer-targeted therapy.
Mechano-boosting nanomedicine antitumour efficacy by blocking the reticuloendothelial system with stiff nanogels
Authors: Li Zheng , Zhu Yabo , Zeng Haowen , Wang Chong , Xu Chen , Wang Qiang , Wang Huimin , Li Shiyou , Chen Jitang , Xiao Chen , Yang Xiangliang , Li Zifu 
Published: March 2023

AbstractNanomedicine has been developed for cancer therapy over several decades, while rapid clearance from blood circulation by reticuloendothelial system (RES) severely limits nanomedicine antitumour efficacy. We design a series of nanogels with distinctive stiffness and investigate how nanogel mechanical properties could be leveraged to overcome RES. Stiff nanogels are injected preferentially to abrogate uptake capacity of macrophages and temporarily block RES, relying on inhibition of clathrin and prolonged liver retention. Afterwards, soft nanogels deliver doxorubicin (DOX) with excellent efficiency, reflected in high tumour accumulation, deep tumour penetration and outstanding antitumour efficacy. In this work, we combine the advantage of stiff nanogels in RES-blockade with the superiority of soft nanogels in drug delivery leads to the optimum tumour inhibition effect, which is defined as mechano-boosting antitumour strategy. Clinical implications of stiffness-dependent RES-blockade are also confirmed by promoting antitumour efficacy of commercialized nanomedicines, such as Doxil and Abraxane.
One-minute sit-to-stand test as a quick functional test for people with COPD in general practice
Authors: Spence J. G. , Brincks J. , Løkke A. , Neustrup L. , Østergaard E. B. 
Published: March 2023

AbstractAssessing changes in functional exercise capacity is highly relevant in the treatment of people with Chronic Obstructive Pulmonary Disease (COPD), as lung function is often static. In Denmark, most people with COPD are followed in general practice where traditional functional tests, like six-minute walk test, require too much time and space. Therefore, there is an urgent need for a quick functional exercise capacity test that can be performed in a limited setting, such as general practice. This study aimed to identify a quick test to measure functional exercise capacity in people with COPD and identify which factors could affect the implementation of such a test in general practice. A mixed method feasibility study composed of a literature review and qualitative interviews was used. Quick functional tests for people with COPD were identified and evaluated through the COSMIN methodology. For the interviews, 64 general practices were included, and 50 staff members and 14 general practitioners (GPs) participated in the interviews. Responses were categorized and thematically analyzed. The 1 min sit-to-stand-test (1 M STST) was found suitable for a general practice setting. The COSMIN methodology rated it “sufficient” in reliability (ICC 0.90–0.99), measurement error (MID 2.5–3), construct validity and responsiveness (AUC 0.72), and found a moderate to strong correlation in criterion validity (r = 0.4–0.75). Several GPs wished for a quick functional test and emphasized evidence, information, and limitations as essential when deciding on implementation. Other factors identified included time, other tests, and economy. 1 M STST is a valid test to assess functional exercise capacity in people with COPD. The test is quick and can easily be performed in a standard consultation, and several GPs wished for such a test.
Differences in HPV-specific antibody Fc-effector functions following Gardasil® and Cervarix® vaccination
Authors: Roy Vicky , Jung Wonyeong , Linde Caitlyn , Coates Emily , Ledgerwood Julie , Costner Pamela , Yamshchikov Galina , Streeck Hendrik , Juelg Boris , Lauffenburger Douglas A. , Alter Galit 
Published: March 2023

AbstractGardasil® (Merck) and Cervarix® (GlaxoSmithKline) both provide protection against infection with Human Papillomavirus 16 (HPV16) and Human Papillomavirus 18 (HPV18), that account for around 70% of cervical cancers. Both vaccines have been shown to induce high levels of neutralizing antibodies and are known to protect against progression beyond cervical intraepithelial neoplasia grade 2 (CIN2+), although Cervarix® has been linked to enhanced protection from progression. However, beyond the transmission-blocking activity of neutralizing antibodies against HPV, no clear correlate of protection has been defined that may explain persistent control and clearance elicited by HPV vaccines. Beyond blocking, antibodies contribute to antiviral activity via the recruitment of the cytotoxic and opsonophagocytic power of the immune system. Thus, here, we used systems serology to comprehensively profile Gardasil®- and Cervarix®- induced antibody subclass, isotype, Fc-receptor binding, and Fc-effector functions against the HPV16 and HPV18 major capsid protein (L1). Overall, both vaccines induced robust functional humoral immune responses against both HPV16 and HPV18. However, Cervarix® elicited higher IgG3 and antibody-dependent complement activating responses, and an overall more coordinated response between HPV16 and 18 compared to Gardasil®, potentially related to the distinct adjuvants delivered with the vaccines. Thus, these data point to robust Fc-effector functions induced by both Gardasil® and Cervarix®, albeit with enhanced coordination observed with Cervarix®, potentially underlying immunological correlates of post-infection control of HPV.
Ultrafast tunable lasers using lithium niobate integrated photonics
Authors: Snigirev Viacheslav , Riedhauser Annina , Lihachev Grigory , Churaev Mikhail , Riemensberger Johann , Wang Rui Ning , Siddharth Anat , Huang Guanhao , Möhl Charles , Popoff Youri , Drechsler Ute , Caimi Daniele , Hönl Simon , Liu Junqiu , Seidler Paul , Kippenberg Tobias J. 
Published: March 2023

AbstractEarly works1 and recent advances in thin-film lithium niobate (LiNbO3) on insulator have enabled low-loss photonic integrated circuits2,3, modulators with improved half-wave voltage4,5, electro-optic frequency combs6 and on-chip electro-optic devices, with applications ranging from microwave photonics to microwave-to-optical quantum interfaces7. Although recent advances have demonstrated tunable integrated lasers based on LiNbO3 (refs. 8,9), the full potential of this platform to demonstrate frequency-agile, narrow-linewidth integrated lasers has not been achieved. Here we report such a laser with a fast tuning rate based on a hybrid silicon nitride (Si3N4)–LiNbO3 photonic platform and demonstrate its use for coherent laser ranging. Our platform is based on heterogeneous integration of ultralow-loss Si3N4 photonic integrated circuits with thin-film LiNbO3 through direct bonding at the wafer level, in contrast to previously demonstrated chiplet-level integration10, featuring low propagation loss of 8.5 decibels per metre, enabling narrow-linewidth lasing (intrinsic linewidth of 3 kilohertz) by self-injection locking to a laser diode. The hybrid mode of the resonator allows electro-optic laser frequency tuning at a speed of 12 × 1015 hertz per second with high linearity and low hysteresis while retaining the narrow linewidth. Using a hybrid integrated laser, we perform a proof-of-concept coherent optical ranging (FMCW LiDAR) experiment. Endowing Si3N4 photonic integrated circuits with LiNbO3 creates a platform that combines the individual advantages of thin-film LiNbO3 with those of Si3N4, which show precise lithographic control, mature manufacturing and ultralow loss11,12.
Blocking NS3–NS4B interaction inhibits dengue virus in non-human primates
Authors: Goethals Olivia , Kaptein Suzanne J. F. , Kesteleyn Bart , Bonfanti Jean-François , Van Wesenbeeck Liesbeth , Bardiot Dorothée , Verschoor Ernst J. , Verstrepen Babs E. , Fagrouch Zahra , Putnak J. Robert , Kiemel Dominik , Ackaert Oliver , Straetemans Roel , Lachau-Durand Sophie , Geluykens Peggy , Crabbe Marjolein , Thys Kim , Stoops Bart , Lenz Oliver , Tambuyzer Lotke , De Meyer Sandra , Dallmeier Kai , McCracken Michael K. , Gromowski Gregory D. , Rutvisuttinunt Wiriya , Jarman Richard G. , Karasavvas Nicos , Touret Franck , Querat Gilles , de Lamballerie Xavier , Chatel-Chaix Laurent , Milligan Gregg N. , Beasley David W. C. , Bourne Nigel , Barrett Alan D. T. , Marchand Arnaud , Jonckers Tim H. M. , Raboisson Pierre , Simmen Kenny , Chaltin Patrick , Bartenschlager Ralf , Bogers Willy M. , Neyts Johan , Van Loock Marnix 
Published: March 2023

AbstractDengue is a major health threat and the number of symptomatic infections caused by the four dengue serotypes is estimated to be 96 million1 with annually around 10,000 deaths2. However, no antiviral drugs are available for the treatment or prophylaxis of dengue. We recently described the interaction between non-structural proteins NS3 and NS4B as a promising target for the development of pan-serotype dengue virus (DENV) inhibitors3. Here we present JNJ-1802—a highly potent DENV inhibitor that blocks the NS3–NS4B interaction within the viral replication complex. JNJ-1802 exerts picomolar to low nanomolar in vitro antiviral activity, a high barrier to resistance and potent in vivo efficacy in mice against infection with any of the four DENV serotypes. Finally, we demonstrate that the small-molecule inhibitor JNJ-1802 is highly effective against viral infection with DENV-1 or DENV-2 in non-human primates. JNJ-1802 has successfully completed a phase I first-in-human clinical study in healthy volunteers and was found to be safe and well tolerated4. These findings support the further clinical development of JNJ-1802, a first-in-class antiviral agent against dengue, which is now progressing in clinical studies for the prevention and treatment of dengue.
The menin inhibitor revumenib in KMT2A-rearranged or NPM1-mutant leukaemia
Authors: Issa Ghayas C. , Aldoss Ibrahim , DiPersio John , Cuglievan Branko , Stone Richard , Arellano Martha , Thirman Michael J. , Patel Manish R. , Dickens David S. , Shenoy Shalini , Shukla Neerav , Kantarjian Hagop , Armstrong Scott A. , Perner Florian , Perry Jennifer A. , Rosen Galit , Bagley Rebecca G. , Meyers Michael L. , Ordentlich Peter , Gu Yu , Kumar Vinit , Smith Steven , McGeehan Gerard M. , Stein Eytan M. 
Published: March 2023

AbstractTargeting critical epigenetic regulators reverses aberrant transcription in cancer, thereby restoring normal tissue function1–3. The interaction of menin with lysine methyltransferase 2A (KMT2A), an epigenetic regulator, is a dependence in acute leukaemia caused by either rearrangement of KMT2A or mutation of the nucleophosmin 1 gene (NPM1)4–6. KMT2A rearrangements occur in up to 10% of acute leukaemias and have an adverse prognosis, whereas NPM1 mutations occur in up to 30%, forming the most common genetic alteration in acute myeloid leukaemia7,8. Here, we describe the results of the first-in-human phase 1 clinical trial investigating revumenib (SNDX-5613), a potent and selective oral inhibitor of the menin–KMT2A interaction, in patients with relapsed or refractory acute leukaemia (ClinicalTrials.gov, NCT04065399). We show that therapy with revumenib was associated with a low frequency of grade 3 or higher treatment-related adverse events and a 30% rate of complete remission or complete remission with partial haematologic recovery (CR/CRh) in the efficacy analysis population. Asymptomatic prolongation of the QT interval on electrocardiography was identified as the only dose-limiting toxicity. Remissions occurred in leukaemias refractory to multiple previous lines of therapy. We demonstrate clearance of residual disease using sensitive clinical assays and identify hallmarks of differentiation into normal haematopoietic cells, including differentiation syndrome. These data establish menin inhibition as a therapeutic strategy for susceptible acute leukaemia subtypes.
A 21-year dataset (2000–2020) of gap-free global daily surface soil moisture at 1-km grid resolution
Authors: Zheng Chaolei , Jia Li , Zhao Tianjie 
Published: March 2023

AbstractGlobal soil moisture estimates from current satellite missions are suffering from inherent discontinuous observations and coarse spatial resolution, which limit applications especially at the fine spatial scale. This study developed a dataset of global gap-free surface soil moisture (SSM) at daily 1-km resolution from 2000 to 2020. This is achieved based on the European Space Agency - Climate Change Initiative (ESA-CCI) SSM combined product at 0.25° resolution. Firstly, an operational gap-filling method was developed to fill the missing data in the ESA-CCI SSM product using SSM of the ERA5 reanalysis dataset. Random Forest algorithm was then adopted to disaggregate the coarse-resolution SSM to 1-km, with the help of International Soil Moisture Network in-situ observations and other optical remote sensing datasets. The generated 1-km SSM product had good accuracy, with a high correlation coefficent (0.89) and a low unbiased Root Mean Square Error (0.045 m3/m3) by cross-validation. To the best of our knowledge, this is currently the only long-term global gap-free 1-km soil moisture dataset by far.
First assessment of underwater sound levels in the Northern Adriatic Sea at the basin scale
Authors: Petrizzo Antonio , Barbanti Andrea , Barfucci Giulia , Bastianini Mauro , Biagiotti Ilaria , Bosi Sofia , Centurelli Michele , Chavanne Robert , Codarin Antonio , Costantini Ilaria , Cukrov Car Marinela , Dadić Vlado , Falcieri Francesco M. , Falkner Raffaela , Farella Giulio , Felli Mario , Ferrarin Christian , Folegot Thomas , Gallou Roger , Galvez Daphnie , Ghezzo Michol , Kruss Aleksandra , Leonori Iole , Menegon Stefano , Mihanović Hrvoje , Muslim Stipe , Pari Alice , Pari Sauro , Picciulin Marta , Pleslić Grgur , Radulović Marko , Rako-Gospić Nikolina , Sabbatini Davide , Soldano Giulia , Tęgowski Jarosław , Vučur-Blazinić Tihana , Vukadin Predrag , Zdroik Jakub , Madricardo Fantina 
Published: March 2023

AbstractThe protection of marine habitats from human-generated underwater noise is an emerging challenge. Baseline information on sound levels, however, is poorly available, especially in the Mediterranean Sea. To bridge this knowledge gap, the SOUNDSCAPE project ran a basin-scale, cross-national, long-term underwater monitoring in the Northern Adriatic Sea. A network of nine monitoring stations, characterized by different natural conditions and anthropogenic pressures, ensured acoustic data collection from March 2020 to June 2021, including the full lockdown period related to the COVID-19 pandemic. Calibrated stationary recorders featured with an omnidirectional Neptune Sonar D60 Hydrophone recorded continuously 24 h a day (48 kHz sampling rate, 16 bit resolution). Data were analysed to Sound Pressure Levels (SPLs) with a specially developed and validated processing app. Here, we release the dataset composed of 20 and 60 seconds averaged SPLs (one-third octave, base 10) output files and a Python script to postprocess them. This dataset represents a benchmark for scientists and policymakers addressing the risk of noise impacts on marine fauna in the Mediterranean Sea and worldwide.
IceLines – A new data set of Antarctic ice shelf front positions
Authors: Baumhoer Celia A. , Dietz Andreas J. , Heidler Konrad , Kuenzer Claudia 
Published: March 2023

AbstractThe frontal position of an ice shelf is an important parameter for ice dynamic modelling, the computation of mass fluxes, mapping glacier area change, calculating iceberg production rates and the estimation of ice discharge to the ocean. Until now, continuous and up-to-date information on Antarctic calving front locations is scarce due to the time-consuming manual delineation of fronts and the previously limited amount of suitable earth observation data. Here, we present IceLines, a novel data set on Antarctic ice shelf front positions to assess calving front change at an unprecedented temporal and spatial resolution. More than 19,400 calving front positions were automatically extracted creating dense inter- and intra-annual time series of calving front change for the era of Sentinel-1 (2014-today). The calving front time series can be accessed via the EOC GeoService hosted by DLR and is updated on a monthly basis. For the first time, the presented IceLines data set provides the possibility to easily include calving front dynamics in scientific studies and modelling to improve our understanding about ice sheet dynamics.
The association of genitourinary cancer among Saudi patients with gastrointestinal stromal tumors and a systematic literature review
Authors: Seyam Raouf , Chaer Kenan Abou , Abouelkhair Ahmed , Almouh Arwa , Alzahrani Othman , Sayed Ahmed Gamal , Alghafees Mohammad , Alhamidi Razan A. , Al-Gonaim Ali , Al-Hussain Turki , Amin Tarek Mahmoud , Altaweel Waleed 
Published: March 2023

AbstractThe gastrointestinal tract's most commonly occurring primary mesenchymal tumor is the gastrointestinal stromal tumor (GIST). However, few cases worldwide were reported associated with renal cell carcinoma (RCC). Therefore, we aimed to identify the association of genitourinary tumors in patients with GIST in our tertiary care hospital in Saudi Arabia and compare it to the literature. We identified all patients in the pathology department database with the diagnosis of GIST. We excluded duplicate and recurrent cases. We examined patients’ files for the presence of RCC, adrenal tumors, or other genitourinary cancer. A systematic review of the association was conducted. From 2003 to 2020, 170 patients had a histopathologic diagnosis of primary GIST, 100 men and 70 women, median age of 57 (range 9–91) years at the time of diagnosis. The site of primary GIST was gastric 103, small bowel 43, mesenteric 5, omentum/peritoneum 7, abdomen 4, isolated adrenal 1, and other 7. Six patients had associated primary genitourinary cancer. Three patients had RCC (two clear cell RCC and one radiologic diagnosis only), and three had adrenal tumors (one adrenal carcinoma, one an isolated adrenal GIST, and one pheochromocytoma). In addition, two patients had a tumor invading the urinary bladder. Although the cohort included 63 men aged 60 or above (median 71 ± 8.7 years, range 60–94), none demonstrated clinical prostatic carcinoma. Data was compared to 69 systematic review articles. We report the rare association between GIST tumors and primary genitourinary cancer, mainly RCC and adrenal tumors. Also, we identified a secondary invasion of the urinary bladder. Unlike the reported series, none of the older male patients had clinical prostate cancer.