bluuebunny/crossref_metadata_2025_split huggingface dataset:
A preliminary study of phosphodiesterases and adenylyl cyclase signaling pathway on red blood cell deformability of sickle cell patients
Authors: Goksel Evrim , Ugurel Elif , Nader Elie , Boisson Camille , Muniansi Ingrid , Joly Philippe , Renoux Celine , Gauthier Alexandra , Connes Philippe , Yalcin Ozlem 
Published: September 2023

Sickle cell disease (SCD) is an inherited hemoglobinopathy characterized by chronic anemia, intravascular hemolysis, and the occurrence of vaso-occlusive crises due to the mechanical obstruction of the microcirculation by poorly deformable red blood cells (RBCs). RBC deformability is a key factor in the pathogenesis of SCD, and is affected by various factors. In this study, we investigated the effects of adenylyl cyclase (AC) signaling pathway modulation and different phosphodiesterase (PDE) modulatory molecules on the deformability and mechanical stress responses of RBC from SCD patients (HbSS genotype) by applying 5 Pa shear stress with an ektacytometer (LORRCA). We evaluated RBC deformability before and after the application of shear stress. AC stimulation with Forskolin had distinct effects on RBC deformability depending on the application of 5 Pa shear stress. RBC deformability was increased by Forskolin before shear stress application but decreased after 5 Pa shear stress. AC inhibition with SQ22536 and protein kinase A (PKA) inhibition with H89 increased RBC deformability before and after the shear stress application. Non-selective PDE inhibition with Pentoxifylline increased RBC deformability. However, modulation of the different PDE types had distinct effects on RBC deformability, with PDE1 inhibition by Vinpocetine increasing deformability while PDE4 inhibition by Rolipram decreased RBC deformability after the shear stress application. The effects of the drugs varied greatly between patients suggesting some could benefit from one drug while others not. Developing drugs targeting the AC signaling pathway could have clinical applications for SCD, but more researches with larger patient cohorts are needed to identify the differences in the responses of sickle RBCs.
Are false positives in suicide classification models a risk group? Evidence for “true alarms” in a population-representative longitudinal study of Norwegian adolescents
Authors: Haghish E. F. , Laeng Bruno , Czajkowski Nikolai 
Published: September 2023

IntroductionFalse positives in retrospective binary suicide attempt classification models are commonly attributed to sheer classification error. However, when machine learning suicide attempt classification models are trained with a multitude of psycho-socio-environmental factors and achieve high accuracy in suicide risk assessment, false positives may turn out to be at high risk of developing suicidal behavior or attempting suicide in the future. Thus, they may be better viewed as “true alarms,” relevant for a suicide prevention program. In this study, using large population-based longitudinal dataset, we examine three hypotheses: (1) false positives, compared to the true negatives, are at higher risk of suicide attempt in future, (2) the suicide attempts risk for the false positives increase as a function of increase in specificity threshold; and (3) as specificity increases, the severity of risk factors between false positives and true positives becomes more similar.MethodsUtilizing the Gradient Boosting algorithm, we used a sample of 11,369 Norwegian adolescents, assessed at two timepoints (1992 and 1994), to classify suicide attempters at the first time point. We then assessed the relative risk of suicide attempt at the second time point for false positives in comparison to true negatives, and in relation to the level of specificity.ResultsWe found that false positives were at significantly higher risk of attempting suicide compared to true negatives. When selecting a higher classification risk threshold by gradually increasing the specificity cutoff from 60% to 97.5%, the relative suicide attempt risk of the false positive group increased, ranging from minimum of 2.96 to 7.22 times. As the risk threshold increased, the severity of various mental health indicators became significantly more comparable between false positives and true positives.ConclusionWe argue that the performance evaluation of machine learning suicide classification models should take the clinical relevance into account, rather than focusing solely on classification error metrics. As shown here, the so-called false positives represent a truly at-risk group that should be included in suicide prevention programs. Hence, these findings should be taken into consideration when interpreting machine learning suicide classification models as well as planning future suicide prevention interventions for adolescents.
Insomnia and common mental disorder among patients with pre-existing chronic non-communicable diseases in southern Ethiopia: a survey during COVID-19 pandemic
Authors: Ayalew Mohammed , Deribe Bedilu , Hussen Siraj , Defar Semira , Tesfaye Emnet , Gedefaw Abel 
Published: September 2023

BackgroundCOVID-19 has been causing significant mental health problems and other health-related issues. Despite the fact that COVID-19 has a significant impact on chronic disease patients, there is scant research on insomnia, common mental health disorders (CMD), and their associated factors among chronic disease patients.ObjectiveThe purpose of this study was to assess the prevalence of insomnia and common mental disorders (CMD) and their associated factors among patients with pre-existing chronic NCDs in Sidama, southern Ethiopia.MethodsA multicenter cross-sectional study was undertaken between June 1 and September 1, 2021. The study included 633 participants. CMD and insomnia were assessed using a 20-item Self-Reported Questionnaire (SRQ-20) and a 7—item Insomnia Severity Index (ISI) scale, respectively. To describe the various variables, descriptive statistics were used. We performed multivariable logistic regression analysis to identify independent factors associated with CMD and insomnia. A value of p &lt; 0.05 was considered statistically significant at a 95% confidence interval.ResultsThe prevalence of insomnia and CMD was found to be 39.3% and 46.8%, respectively. Being merchant (AOR = 0.33; 95% CI = 0.13, 0.82), having a diagnosis of diabetes mellitus (AOR = 1.89; 95% CI = 1.04, 3.46), comorbid diagnosis (AOR = 3.96; 95% CI = 2.27, 6.89), low social support (poor (AOR = 3.37; 95% CI = 1.51, 7.57) and moderate (AOR = 3.13; 95% CI = 1.46, 6.69)), symptoms of insomnia (AOR = 12.08; 95% CI = 7.41, 19.72) and poor quality of life (QOL) (AOR = 1.67; 95% CI = 1.04, 2.72) were independent predictors of CMD. We also found out that, having cardiovascular disorders (CVDs) (AOR = 2.48; 95% CI = 1.18, 5.19), CMD (AOR = 12.09; 95% CI = 7.46, 19.61), and poor QOL (AOR = 2.04; 95% CI = 1.27, 3.26) were significantly associated with insomnia symptoms.ConclusionOur study suggests that substantially high prevalence of CMD and insomnia. Significant association between CMD and occupation, diagnosis, comorbidity, social support, insomnia, and QOL were found. We also revealed that having CVDs, CMD, and poor QOL were significantly associated with insomnia symptoms. Therefore, dealing with the mental health problems of patients with chronic NCDs is an essential component of public health intervention during the COVID-19 pandemic.
Suicidal ideation, attempt and associated factors among people with cancer attending cancer center, eastern Ethiopia
Authors: Nigussie Kabtamu , Tesfaye Dejene , Abdisa Lemesa , Tolosa Lidiya , Bete Tilahun , Gemechu Kabtamu , Negash Abraham , Sertsu Addisu , Dereje Jerman , Debele Gebiso Roba , Dechasa Deribe Bekele 
Published: September 2023

BackgroundSuicide is one of the most common public health problems and the second leading cause of death among individuals 15–29 years of age. Suicidal ideation and attempt are one of the common psychiatric emergence in patients with cancer that needs early detection and management before patients end their lives. Therefore, the aim of the study was to assess the magnitude of suicidal ideation, attempts, and associated factors among people with cancer in Eastern Ethiopia.MethodsAn institutional-based cross-sectional study design was conducted among 362 participants. A composite international diagnostic interview was used to evaluate suicidal ideation and attempt. Epi-Data version 4.6.2 was used for data entry, and SPSS version 20 was used for analysis. Logistic regression analysis was done to identify associated factors for both suicidal ideation and attempt. p-values less than 0.05 are considered statistically significant, and the strength of the association will be represented by an adjusted odds ratio with a 95% confidence interval.ResultsThe magnitude of suicidal ideation and attempt among people with in this study was 22.9% [95% CI, 18.7–27.4] and 9.8% [95%CI, 6.7–12.8] respectively. Being living alone [AOR = 4.90, 95% CI, 2.08–11.90], and having depressive symptoms [AOR = 3.28, 95% CI, 1.37–7.73], female ([AOR = 1.53, 95% CI, 1.30–3.23], anxiety symptoms [AOR = 3.06, 95% CI, 1.35–6.73)] and having poor social support [AOR = 3.08, 95% CI, 1.72–5.05], were significantly associated suicidal ideation whereas, Being living alone [AOR = 2.89, 95% CI, 1.09–7.65], having a depressive symptoms [AOR = 4.88, 95% CI, 1.45–13.28], being divorced/widowed [AOR = 3.46, 95% CI, 1.09–10.09] and stage four cancer [AOR = 5.53, 95% CI 2.97–7.47] were significantly associated with suicidal attempt.ConclusionNearly one-quarter and one-tenth of people with cancer have suicide ideation and attempt, respectively. Suicidal behavior among cancer patients was found in this study to be a common problem. Living alone, having depressive and anxiety symptoms, being a female, having poor social support, and being in forth stage of cancer were risk factors for suicide. Therefore, early screening, diagnosing, and treating suicide and its factors will be mandatory and expected from health care providers and non-governmental organizations.
Efficacy and safety of cannabidiol for the treatment of canine osteoarthritis: a systematic review and meta-analysis of animal intervention studies
Authors: Patikorn Chanthawat , Nerapusee Osot , Soontornvipart Kumpanart , Lawonyawut Kanta , Musikpodok Kachapong , Waleethanaphan Kanisorn , Anantachoti Puree 
Published: September 2023

IntroductionCanine osteoarthritis (OA) is a degenerative disease with chronic inflammation of internal and external joint structures in dogs.Cannabisspp. contains cannabidiol (CBD), a substance known for various potential indications, such as pain relief and anti-inflammatory in various types of animals, including dogs with OA. As CBD is increasingly in the spotlight for medical use, we aimed to perform a systematic review and meta-analysis to evaluate the efficacy and safety of CBD in treating canine OA.MethodsWe searched PubMed, Embase, Scopus, and CAB Direct for animal intervention studies investigating the effects of CBD for canine OA from database inception until February 28, 2023. Study characteristics and findings were summarized. A risk of bias in the included studies was assessed. Meta-analyses were performed using a random-effects model to estimate the effects of CBD on pain scores (0–10), expressed as mean difference (MD) and 95% confidence interval (95% CI). Certainty of evidence was assessed using GRADE.ResultsFive articles were included, which investigated the effects of CBD in 117 dogs with OA. All studies were rated as having a high risk of bias. CBD products varied substantially, i.e., oral full-spectrum CBD oil in four studies, and isolated CBD oil and liposomal CBD oil in another study. Treatment duration varied from 4–12 weeks. Meta-analyses of three studies found that, in dogs with OA, treatment with oral full-spectrum CBD oil may reduce pain severity scores (MD; −0.60, 95% CI; −1.51 to 0.31,I2= 45.64%,p = 0.19) and pain interference scores (MD; −1.52, 95% CI; −3.84 to 0.80,I2= 89.59%,p = 0.20) but the certainty of evidence was very low. CBD is generally considered safe and well-tolerated in the short-run, with few mild adverse events observed, such as vomiting and asymptomatic increase in alkaline phosphatase level.ConclusionCBD is considered safe for treating canine OA. CBD may reduce pain scores, but the evidence is very uncertain to conclude its clinical efficacy. High-quality clinical trials are needed to further evaluate the roles of CBD in canine OA.
Interaction of Garcinia cambogia (Gaertn.) Desr. and Drugs as a Possible Mechanism of Liver Injury: The Case of Montelukast
Authors: Di Giacomo Silvia , Di Sotto Antonella , Percaccio Ester , Scuotto Erica , Battistelli Cecilia , Mazzanti Gabriela , Menniti-Ippolito Francesca , Ippoliti Ilaria 
Published: September 2023

Overweight and obesity prevalence has increased worldwide. Apart from conventional approaches, people also resort to botanical supplements for reducing body weight, although several adverse events have been associated with these products. In this context, the present study aimed at evaluating the toxicity of Garcinia cambogia-based products and shedding light on the mechanisms involved. The suspected hepatotoxic reactions related to G. cambogia-containing products collected within the Italian Phytovigilance System (IPS) were examined. Then, an in vitro study was performed to evaluate the possible mechanisms responsible for the liver toxicity, focusing on the modulation of oxidative stress and Nrf2 expression. From March 2002 to March 2022, the IPS collected eight reports of hepatic adverse reactions related to G. cambogia, which exclusively involved women and were mostly severe. The causality assessment was probable in three cases, while it was possible in five. In the in vitro experiments, a low cytotoxicity of G. cambogia was observed. However, its combination with montelukast greatly reduced cell viability, increased the intracellular ROS levels, and affected the cytoplasmic Nrf2 expression, thus suggesting an impairment of the antioxidant and cytoprotective defenses. Overall, our results support the safety concerns about G. cambogia-containing supplements and shed light on the possible mechanisms underpinning its hepatotoxicity.
A New Chengjiang Worm Sheds Light on the Radiation and Disparity in Early Priapulida
Authors: Wang Deng , Vannier Jean , Sun Jie , Yu Chiyang , Han Jian 
Published: September 2023

The vast majority of early Paleozoic ecdysozoan worms are often resolved as stem-group Priapulida based on resemblances with the rare modern representatives of the group, such as the structure of the introvert and the number and distribution of scalids (a spiny cuticular outgrowth) and pharyngeal teeth. In Priapulida, both scalids and teeth create symmetry patterns, and three major diagnostic features are generally used to define the group: 25 longitudinal rows of scalids (five-fold symmetry), 8 scalids around the first introvert circle and the pentagonal arrangement of pharyngeal teeth. Here we describe Ercaivermis sparios gen. et sp. nov., a new priapulid from the early Cambrian Chengjiang Lagerstätte, characterized by an annulated trunk lacking a sclerotized ornament, four pairs of anal hooks and 16 longitudinal rows of scalids along its introvert and eight scalids around each introvert circle, giving the animal an unusual octoradial symmetry. Cladistic analyses resolve Ercaivermis as a stem-group priapulid. Ercaivermis also suggests that several biradial symmetry patterns (e.g., pentagonal, octagonal) expressed in the cuticular ornament, may have co-existed among early Cambrian priapulids and that the pentaradial mode may have become rapidly dominant during the course of evolution, possibly via the standardization of patterning, i.e., the natural selection of one symmetry type over others.
Involvement of Nucleotide Excision Repair and Rec-Dependent Pathway Genes for UV Radiation Resistance in Deinococcus irradiatisoli 17bor-2
Authors: Subramani Gayathri , Srinivasan Sathiyaraj 
Published: September 2023

Strain Deinococcus irradiatisoli 17bor-2 was isolated from a soil sample exposed to γ radiation at Seoul Women’s University, Republic of Korea. The genus Deinococcus is a Gram-negative, coccus-shaped, and extremophilic bacterium, well renowned as being a radiation-resistant bacterium. Therefore, the mechanism behind the resistance to radiation and the gene responsible for the resistance could be helpful for detailed experimental studies with biotechnological applications. To study the involvement of genes in UV radiation resistance in strain 17bor-2, the genomic DNA of the strain was sequenced and constructed using the Pacific Biosciences RS II system. In addition, the complete genome sequence of strain 17bor-2 was annotated and interpreted using the Genomes–Expert Review (IMG-ER) system, along with Prodigal and JGI GenePRIMP analysis. The genome analysis of strain 17bor-2 revealed evidence of excinuclease UvrABC genes, which are key enzymes in the nucleotide excision repair (NER) mechanism, as well as genes from the recA-dependent and recQ pathways. The genome of strain Deinococcus irradiatisoli 17bor-2 was a circular chromosome comprising 3,052,043 bp with a GC content of 67.0%, including 2911 coding sequences (CDs), 49 tRNA genes, and 9 rRNA genes. In addition, their complete genome sequence annotation features provided evidence that radiation resistance genes play a central part in adaptation against extreme environmental conditions. In recent decades, excision repair genes have been indicated in considerable detail for both prokaryote and eukaryote resistance against UV-C radiation.
Modeling Myotonic Dystrophy Type 2 Using Drosophila melanogaster
Authors: Marzullo Marta , Coni Sonia , De Simone Assia , Canettieri Gianluca , Ciapponi Laura 
Published: September 2023

Myotonic dystrophy 2 (DM2) is a genetic multi-systemic disease primarily affecting skeletal muscle. It is caused by CCTGn expansion in intron 1 of the CNBP gene, which encodes a zinc finger protein. DM2 disease has been successfully modeled in Drosophila melanogaster, allowing the identification and validation of new pathogenic mechanisms and potential therapeutic strategies. Here, we describe the principal tools used in Drosophila to study and dissect molecular pathways related to muscular dystrophies and summarize the main findings in DM2 pathogenesis based on DM2 Drosophila models. We also illustrate how Drosophila may be successfully used to generate a tractable animal model to identify novel genes able to affect and/or modify the pathogenic pathway and to discover new potential drugs.
Enterobacter Strain IPPBiotE33 Displays a Synergistic Effect with Bacillus thuringiensis Bt185
Authors: Mi Liang , Gu Ziqiong , Li Ying , Xu Wenyue , Shu Changlong , Zhang Jie , Bai Xi , Geng Lili 
Published: September 2023

The discovery and isolation of new non-Bt insecticidal bacteria and genes are significant for the development of new biopesticides against coleopteran pests. In this study, we evaluated the insecticidal activity of non-Bt insecticidal bacteria, PPBiotE33, IPPBiotC41, IPPBiotA42 and IPPBiotC43, isolated from the peanut rhizosphere. All these strains showed insecticidal activity against first- and third-instar larvae of Holotrichia parallela, Holotrichia oblita, Anomala corpulenta and Potosia brevitarsis. IPPBiotE33 showed the highest toxicity among the four strains and exhibited virulence against Colaphellus bowringi. The genome of IPPBiotE33 was sequenced, and a new protein, 03673, with growth inhibition effects on C. bowringi was obtained. In addition, IPPBiotE33 had a synergistic effect with Bacillus thuringiensis Bt185 against H. parallela in bioassays and back-inoculation experiments with peanut seedlings. IPPBiotE33 induced a decrease in hemocytes and an increase in phenol oxidase activity in H. parallela hemolymph, known as the immunosuppressive effect, which mediated synergistic activity with Bt185. This study increased our knowledge of the new insecticidal strain IPPBiotE33 and shed new light on the research on new insecticidal coaction mechanisms and new blended pesticides.
First Record of the Genus Cartorhynchites Voss, 1958 (Coleoptera: Rhynchitidae) from Eocene Baltic Amber with a List of Fossil Tooth-Nosed Snout Weevils
Authors: Legalov Andrei A. , Bukejs Andris , Vanaga Anarina , Alekseev Vitalii I. 
Published: September 2023

A new species of the genus Cartorhynchites (Rhynchitini, Rhynchitina) is described from Baltic amber. Cartorhynchites groehni Legalov, Bukejs et Alekseev sp. n. differs from C. struvei Zherikhin, 1992 from the Miocene of Germany in its smaller body size (2.6 mm), strongly convex eyes, narrower pronotum and wide elytra, and dark brown legs. A new species is studied and illustrated in detail using X-ray micro-computed tomography (μCT). It is the earliest fossil record of subtribe Rhynchitina. A list of fossil Rhynchitidae was compiled. A key to species of Rhynchitidae in Baltic amber was given. Fossil finds of the family Rhynchitidae were discussed. The assumption was made that the Recent distribution range of the genus Cartorhynchites is within the range of its host plant of the genus Symplocos. Probably, a new Eocene species developed on Symplocos kowalewskii, and the Oligocene C. struvei was associated with Symplocos myosotis (Unger).
ANALISIS USER EXPERIENCE APLIKASI OJEK ONLINE MAXIM DENGAN MODEL HCI
Authors: Mulyadi Lya , Fitrianti Ika , Ridha Azhari Ali 
Published: September 2023

Ojek online merupakan salah satu bentuk dari perkembangan teknologi yang sudah marak di Indonesia. pada pengembangan aplikasinya pun, sudah banyak start up yang memiliki layanan dengan keunggulan mereka masing-masing. salah satu layanan ojek online yangmulai banyak diminati ini adalah Maxime. Penggunaan aplikasi ini juga pasti tidak terlepas dari tampilan user interface yang dapat membantu pengguna dengan maksimal. Dalam salah satu aplikasi yang menawarkan kemudahan untuk melaksanakan layanan ojek online ini, masih memiliki beberapa kekurangan pada user interface yang diberikan. Pada jurnal ini, akan dijelaskan apa saja yang memiliki kekurangan dan yang perlu untuk dapat memaksimalkan tampilan dari user interface pada aplikasi Maxime dengan menggunakan model pendekatan HCI dan metode kuisioner untuk dapat mengumpulkan serta menyimpulkan kebutuhan pengguna dan calon pengguna. Peneliti memfokuskan pada beberapa prinsip dari desain interface itu sendiri seperti Eficiency, Consistency, Ese of Use, User compability, ease of learning.
Kata Kunci: Aplikasi, User interface, Ojek Online, HCI