bluuebunny/crossref_metadata_2025_split huggingface dataset:
Integrating single-cell RNA-seq and spatial transcriptomics reveals MDK-NCL dependent immunosuppressive environment in endometrial carcinoma
Authors: Yu Xinnian , Xie Linjun , Ge Jianjuan , Li Huixin , Zhong Shanliang , Liu Xiaolin 
Published: April 2023

ObjectivesThe tumor microenvironment (TME) play important roles in progression of endometrial carcinoma (EC). We aimed to assess the cell populations in TME of EC.MethodsWe downloaded datasets of single-cell RNA-seq (scRNA-seq) and spatial transcriptome (ST) for EC from GEO, and downloaded RNA-Seq (FPKM) and clinical data of TCGA-UCEC project from TCGA. The datasets were analyzed using R software.ResultsWe obtained 5 datasets of scRNA-seq, 1 of ST and 569 samples of RNA-seq. Totally, 0.2 billion transcripts and 33,408 genes were detected in 33,162 cells from scRNA-seq. The cells were classified into 9 clusters, and EC cells were originated from epithelial cells and ciliated cells. Gene set variation analysis (GSVA) indicated that the pathways enriched in the subclusters of epithelial cells and endothelial cells were significantly different, indicating great heterogeneity in EC. Cell-cell communication analyses showed that EC cells emitted the strongest signals, and endothelial cells received more signals than other cells. Further analysis found that subclusters of 1 and 2 of epithelial cells were showed a more malignant phenotype, which may confer malignant phenotype to subcluster of 0 of endothelial cells through MK pathway by MDL-NCL signal. We also analyzed communications between spatial neighbors with ST data and confirmed the findings on MDL-NCL in cell-cell communication. TCGA and GEO analyses indicated that the expression levels of NCL was inversely correlated with ImmuneScore.ConclusionOur study revealed EC cells can confer malignant phenotype to endothelial cells by MDK-NCL signal, and NCL is associated with suppressed immune activity. EC cells may shape TME by inhibiting immune cells and “educating” stromal cells via MDK-NCL signal.
Case report: Superficial cervicovaginal myofibroblastoma of the cervix with endometrial carcinoma
Authors: Zhang Xiaowei , Huang Bifei , Du Junqiang 
Published: April 2023

IntroductionA superficial cervical vaginal myofibroblastoma is a rare mesenchymal tumor, originating from the superficial stroma of the vagina and cervix. This study reports a patient, who was diagnosed with endometrioid carcinoma and a concomitant benign superficial cervicovaginal myofibroblastoma.Case presentationA 53-year-old female with endometrial carcinoma was admitted to the Department of Gynecology of our hospital. She had a history of breast cancer on May 23, 2010, and took toremifene citrate for 41 months. Radical resection of the endometrial carcinoma was performed at our hospital. Based on the pathological findings, she was postoperatively diagnosed with endometrial adenocarcinoma with superficial cervical vaginal myofibroblastoma. The patient continued receiving postoperative breast cancer treatment. She underwent follow-up for 23 months. No recurrence or metastasis of the endometrial cancer or superficial cervical vaginal myofibroblastoma was observed.ConclusionThere were similarities between superficial cervical vaginal myofibroblastoma and other mesenchymal tumors of the female genital tract. Superficial cervical vaginal myofibroblastomas have a good prognosis, and the combination of tissue morphology and immunohistochemistry helped establish a definitive diagnosis.
Negative and positive allosteric modulators of the α7 nicotinic acetylcholine receptor regulates the ability of adolescent binge alcohol exposure to enhance adult alcohol consumption
Authors: Rodd Zachary A. , Swartzwelder H. Scott , Waeiss R. Aaron , Soloviov Serhii O. , Lahiri Debomoy K. , Engleman Eric A. , Truitt William A. , Bell Richard L. , Hauser Sheketha R. 
Published: April 2023

Rationale and Objectives: Ethanol acts directly on the α7 Nicotinic acetylcholine receptor (α7). Adolescent-binge alcohol exposure (ABAE) produces deleterious consequences during adulthood, and data indicate that the α7 receptor regulates these damaging events. Administration of an α7 Negative Allosteric Modulator (NAM) or the cholinesterase inhibitor galantamine can prophylactically prevent adult consequences of ABAE. The goals of the experiments were to determine the effects of co-administration of ethanol and a α7 agonist in the mesolimbic dopamine system and to determine if administration of an α7 NAM or positive allosteric modulator (PAM) modulates the enhancement of adult alcohol drinking produced by ABAE.Methods: In adult rats, ethanol and the α7 agonist AR-R17779 (AR) were microinjected into the posterior ventral tegmental area (VTA), and dopamine levels were measured in the nucleus accumbens shell (AcbSh). In adolescence, rats were treated with the α7 NAM SB-277011-A (SB) or PNU-120596 (PAM) 2 h before administration of EtOH (ABAE). Ethanol consumption (acquisition, maintenance, and relapse) during adulthood was characterized.Results: Ethanol and AR co-administered into the posterior VTA stimulated dopamine release in the AcbSh in a synergistic manner. The increase in alcohol consumption during the acquisition and relapse drinking during adulthood following ABAE was prevented by administration of SB, or enhanced by administration of PNU, prior to EtOH exposure during adolescence.Discussion: Ethanol acts on the α7 receptor, and the α7 receptor regulates the critical effects of ethanol in the brain. The data replicate the findings that cholinergic agents (α7 NAMs) can act prophylactically to reduce the alterations in adult alcohol consumption following ABAE.
miRNA deregulation and relationship with metabolic parameters after Mediterranean dietary intervention in BRCA-mutated women
Authors: De Summa Simona , Traversa Debora , Daniele Antonella , Palumbo Orazio , Carella Massimo , Stallone Raffaella , Tufaro Antonio , Oliverio Andreina , Bruno Eleonora , Digennaro Maria , Danza Katia , Pasanisi Patrizia , Tommasi Stefania 
Published: April 2023

BackgroundBreast cancer onset is determined by a genetics-environment interaction. BRCA1/2 gene alterations are often genetically shared in familial context, but also food intake and hormonal assessment seem to influence the lifetime risk of developing this neoplasia. We previously showed the relationship between a six-months Mediterranean dietary intervention and insulin, glucose and estradiol levels in BRCA1/2 carrier subjects. The aim of the present study was to evidence the eventual influence of this dietary intervention on the relationship between circulating miRNA expression and metabolic parameters in presence of BRCA1/2 loss of function variants.MethodsPlasma samples of BRCA-women have been collected at the baseline and at the end of the dietary intervention. Moreover, subjects have been randomized in two groups: dietary intervention and placebo. miRNA profiling and subsequent ddPCR validation have been performed in all the subjects at both time points.ResultsddPCR analysis confirmed that five (miR-185-5p, miR-498, miR-3910, miR-4423 and miR-4445) of seven miRNAs, deregulated in the training cohort, were significantly up-regulated in subjects after dietary intervention compared with the baseline measurement. Interestingly, when we focused on variation of miRNA levels in the two timepoints, it could be observed that miR-4423, miR-4445 and miR-3910 expressions are positively correlated with variation in vitaminD level; whilst miR-185-5p difference in expression is related to HDL cholesterol variation.ConclusionsWe highlighted the synergistic effect of a healthy lifestyle and epigenetic regulation in BC through the modulation of specific miRNAs. Different miRNAs have been reported involved in the tumor onset acting as tumor suppressors by targeting tumor-associated genes that are often downregulated.
Using the Social Skills Improvement System (SSiS) Rating Scales to assess social skills in youth with Down syndrome
Authors: Channell Marie Moore , Mattie Laura J. , Schworer Emily K. , Fidler Deborah J. , Esbensen Anna J. 
Published: April 2023

Introduction and MethodsThis study provides preliminary data on the Social Skills Improvement System (SSiS) Rating Scales Parent Form to measure social skills in a sample of 124 children and adolescents with Down syndrome (DS) ages 6–17 years.ResultsOverall, participants demonstrated relatively mild symptoms, with the sample’s average standard score falling within 1 standard deviation from the mean of the normative sample for the social skills (M = 92, SD = 15) and problem behaviors (M = 104, SD = 12) domains (normative sample M = 100, SD = 15 for both domains). However, a wide range of scores was observed across the sample for the composite and subscale scores. Differential patterns were also observed by subscale. For some subscales (i.e., Cooperation, Assertion, Responsibility, Engagement, Externalizing, Hyperactivity/Inattention, and Autism Spectrum), a disproportionate number of participants scored in the below average (i.e., lower levels of social skills) or above average (i.e., more symptomatic in problem behaviors or autism spectrum) range relative to the normative sample; for other subscales (i.e., Communication, Empathy, Self-Control, Bullying, and Internalizing), participants’ score distribution aligned more closely to that of the normative sample. SSiS composite scores correlated in the expected directions with standardized measures of autism characteristics, executive function, and expressive language.DiscussionThis study provides some of the first evidence validating the use of the SSiS in youth with DS, filling a gap in standardized measures of social functioning in this population.
Cluster analysis for the overall health status of elderly, multimorbid patients with diabetes
Authors: Bing Yan , Yuan Lei , Liu Ji , Wang Zezhong , Chen Lifu , Sun Jinhai , Liu Lijuan 
Published: April 2023

PurposeTo evaluate the overall health status and health-related abilities and problems of elderly patients with diabetes and multimorbidity compared with those with diabetes only. Additionally, we aimed to identify different subgroups of elderly, multimorbid patients with diabetes.MethodsThis cross-sectional study included 538 elderly patients with diabetes. The participants completed a series of questionnaires on self-rated health (SRH), diabetes self-management, self-efficacy, health literacy, depression, and diabetes distress. Differences in health-related abilities and problems were compared between elderly patients with diabetes and multimorbidity and those with diabetes only, with adjustments for covariates using propensity score matching. A cluster analysis was also performed to identify the overall health status subgroups of elderly, multimorbid patients with diabetes. Additionally, we conducted a multinomial logistic regression analysis to examine the predictors of health-related abilities and problem-cluster group membership.ResultsElderly patients with diabetes and multimorbidity experienced more health-related abilities and problems than those with diabetes only, particularly within the domains of depression (p &lt; 0.001), and diabetes distress. The level of health literacy (p &lt; 0.001) and self-management (p = 0.013) in elderly, multimorbid patients with diabetes was also significantly higher than that in elderly patients with diabetes only. Cluster analysis of elderly, multimorbid patients with diabetes revealed three distinct overall health status clusters. Multinomial logistic regression analysis indicated that age (OR = 1.090, p = 0.043), sex (OR = 0.503, p = 0.024), living situation (OR = 2.769, p = 0.011), BMI (OR = 0.838, p = 0.034), regular exercise (OR = 2.912, p = 0.041 in poor vs. good; OR = 3.510, p &lt; 0.001 in intermediate vs. good), and cerebral infarction (OR = 26.280, p &lt; 0.001) independently and significantly predicted cluster membership.ConclusionCompared with elderly patients with diabetes only, those with diabetes and multimorbidity experienced more health-related abilities and problems within the domains of depression, and diabetes distress. Additionally, the level of health literacy and self-management in elderly, multimorbid patients with diabetes was significantly higher than that in those with diabetes only. Among the multimorbid diabetes group, old age, male sex, living without a partner, slightly lower BMIs, not exercising regularly, and experiencing cerebral infarctions were all positively correlated with worse overall health status.
Spinal dural and epidural arteriovenous fistula: Recurrence rate after surgical and endovascular treatment
Authors: Vercelli Giovanni Giulio , Minardi Massimiliano , Bergui Mauro , Zenga Francesco , Garbossa Diego , Cofano Fabio 
Published: April 2023

IntroductionSpinal dural arteriovenous fistula consist of an heterogenous group of vascular malformation often causing severe neurological deficit due to progressive myelopathy. This type of malformation could be associated with subarachnoid or subdural hemorrhage inside the spinal canal. In the English literature surgical treatment is regarded as the best option if compared to endovascular procedure, being the latter associated with an increased risk of relapse despite its less invasiveness.MethodsIn this study a retrospective analysis of 30 patients with spinal dural and epidural fistula associated with perimedullary venous congestion was undertaken. The radiological and clinical presentation of each patient is analyzed, and the grade of myelopathy is classified through the mJOA score.ResultsA total number of 31 out of 41 collected procedures (22 surgery vs. 19 endovascular) were dural fistulas while the remaining 10 were classified as epidural. A 46% recurrence rate for endovascular treatment against 0% for surgical (p-value 0.004) was described for dural fistulas, while in the epidural fistula group the rate of recurrence was 80% and 20% respectively for endovascular and surgery treatment (p-value 0.6).DiscussionAccording to the results, surgical treatment could be considered as first-line treatment for spinal dural arteriovenous fistulas. Endovascular embolization can be proposed in selected cases, as a less invasive technique, for elderly patients or with important comorbidities. In spinal epidural arteriovenous fistulas, in view of the greater invasiveness of the surgical treatment and the non-significant difference in terms of recurrence risk between the two techniques, endovascular treatment could be proposed as a first choice treatment; in the event of a recurrence, a surgical intervention will instead be proposed in a short time.
The Mediating Role of Depression in Association Between Total Sleep Time and Instrumental Activities of Daily Living in China
Authors: Wu Yunyi , Li Sangsang , Han Dan , Zhang Mei , Zhao Jie , Liao Hui , Ma Ying , Yan Chaoyang , Wang Jing 
Published: April 2023

Objectives: This study aims to investigate the mediating role of depression and the moderating effect of gender in the relationship between total sleep time (TST) and instrumental activities of daily living (IADL) in middle-aged and elderly people (aged 45 or above).Methods: The data used in this study is from the China Health and Retirement Longitudinal Study (CHARLS), including a total of 10,460 respondents. Associations between TST, IADL, depression, and gender were analyzed using logistic regression and Karlson, Holm, and Breen (KHB) methods.Results: Short (OR = 1.42, 95% CI = 1.28–1.58 of ≤6 h) and long TST (OR = 1.16, 95% CI = 1.02–1.32 of 8–9 h; OR = 1.35, 95% CI = 1.19–1.54 of &gt;9 h) were both associated with IADL. The mediation effect analyses observed that depression explained 64.80% of the total effect of short TST (≤6 h) and IADL, but was insignificant in long TST (8–9 h and &gt;9 h). Meanwhile, gender has moderating effects on the mediation effect model.Conclusion: The study suggests that health interventions that focused on the dimensions of TST and depression are crucial for preventing functional disability while accounting for gender differences.
Sur la prévalence féminine de l'anorexie mentale
Authors: Brusset Bernard 
Published: April 2023

Bernard Brusset, On female prevalence in anorexia nervosa.
To explain the remarkable female predominance in anorexia nervosa, one usually stays within general platitudes, putting forward a vague "plurifactorial determinism", or unduly privileging one type of factor over others. The confrontation of biological, epidemiological and historical data with clinical processes and with data from psychoanalytic psychotherapies enables us to try to specify how, and by which mechanism, different factors intervene in the anorectic process. Evaluating the possible action of biological, cultural, family factors that can be more or less present depending on the case, all the more emphasises the importance of the problematic of adolescence, and of certain specificities of the girl's libidinal development.

Key-words : Determinism, Process, Libidinal development, Anorexia nervosa.
Féminité et filiation : l'épreuve du temps
Authors: Béhar-Azoulay Catherine 
Published: April 2023

Catherine Béhar-Azoulay, Feminidad y filiación : la prueba del tiempo.
Este artículo expone un cierto numéro de reflexiones que conciernen la relación al tiempo de la jovencita confrontada al proceso de fin de la adolescencia y que debe realizar elecciones que implican su vida presente y sobre todo futura. Retomando algunas referencias esenciales de autores que han trabajado sobre la adolescencia, la identidad y el tiempo, el autor pone el acento sobre la importancia del anclaje del sujeto en su filiación para que un proyecto de existencia pueda madurar, luego concretarse. Es el arraigo de un sujeto en su filiation que le permite, ante todo, conjugar su existencia por siempre, asegurar su continuidad y tomar a cargo una historia personal. La joven entre 18 y 22 anos está, tal vez, más solicitada en ese sentido que el joven, en la medida que el tiempo "femenino" se comprende en referencia al espacio-tiempo de la maternidad. Estos propósitos sern ilustrados a través de ejemplos de producciones en las pruebas proyectivas del Rorschach y del TAT.

Palabras-clave : Adolescencia, Tiempo, Rorschach, TAT
Un penser, « royaume de l'entre-deux »
Authors: Noaille Pierre 
Published: April 2023

Pierre Noaille, Un pensamiento, "reino del entre-dos".
"Cuestión límite", a primera vista, para un psicoanálisis identificado en tanto que "ciencia de las profundidades" enteramente centrado sobre ese "núcleo" que es el inconsciente, el pensamiento puede, en efecto, encontrar su lugar en la periferia, tendremos por lo tanto que reconocer que el proyecto freudiano hace de ese "límite" su ideal, el punto de perspectiva a alcanzar implícitamente. Un límite - "la envoltura" - en lo que él implica de "síntesis", que el psicoanálisis parece no obstante temer en tanto que él apuesta sobre su intervención. Es que el psicoanálisis no sería otra cosa que un movimiento de pensamiento que tiene esa particularidad de problematizar su propio movimiento ? El lo sitúa, y haciéndose, sitúa "su" propio campo, precisamente en el "entre-dos" del par solidario "núcleo"-"envoltura". La "categoría de lo intermediario" - epistemología fundadora para el campo freudiano - se désigna así como el elemento conceptual verdaderamente apropiado a una problemática del proceso de pensamiento.

Palabras-clave : Pensamiento, Psicoanálisis freudiano, Categoría de lo intermediario, Narcisismo.
Inceste et maltraitance : étude comparative au Rorschach
Authors: Montreuil Michèle , Dubus Chantal , Castelain Bernadette , Elipot Françoise 
Published: April 2023

Michèle Montreuil, Incest and maltreatment : comparative study with Rorschach.
We met 17 women in an association called "aid to victims". These women had suffered from maltreatment in childhood. Our method was based on clinical interviews and on Rorschach. We notice that identity and identification are fragile. The variable degree of fragility does not seem to be linked to the trauma itself but appears to be at the origin of depression. For some women victims of childhood incest, clinical interview and projective tests show confusion in family roles and generations and the reexperiencing of the sexual trauma in the present. In reference to Freud's theory on the second trauma, sexual abuse could constitute a screen trauma, hiding the first trauma related to primary maternal negligence or maltreatment.

Key-words : Childhood maltreatment, incest, maternal neglect, long-term trauma effect, clinical study.
Violence et adolescence. De la violence dans « la galère » à une réflexion métapsychologique sur la violence à l'adolescence
Authors: Feder Françoise 
Published: April 2023

Françoise Feder, Violence and adolescence. From the violence of life on the skids ("la galère") to a metapsychological reflection about violence during adolescence.
The text presented here illustrates various aspects of violence. Basing her research on a sociological study of the phenomenon of life on the skids ("la galère") among young people, the author draws her inspiration from this model and employs it as a metaphor to be applied at the clinical level to borderline pathologies of adolescence.
Herein, she will attempt to establish the metapsychological status of violence on a theoretical as well as a clinical level in the domains of neurosis and of borderline organizations, through the works of Freud and of other authors, by inquiring into the nature, the place and the destinies of aggressivity in the psychic apparatus.
This reflection, which takes environmental conditions into account in the construction of the psyche, gives a central position to the notion of the drive object in its function of an objectal limit which may have proved ineffective in certain cases of violence.

Key-words : violence, adolescence, the skids (« galère »), environment, limit, Superego, narcissism, object, drive.
Violence de l'adolescent et angoisse de démembrement
Authors: Nevjinsky Fern 
Published: April 2023

Fern Nevjinsky, Adolescent violence and dismemberment anxiety.
By following the evolution of approximately forty adolescents for the past few years, we have been able to determine the signs which are frequently linked to the occurrence of violent acts. Starting from the interpretation of these data, the author has hypothesized that for these adolescents, the work of mourning cannot be accomplished normally and is replaced by a work of melancholy which appears during a massive narcissistic regression. The encounter between aggressive maternal introjects and the presence of a "clinging" maternal imago can generate "dismemberment anxiety", a consequence of instinctual disentanglement and the failure to establish a primary erogenous masochistic core.

Key-words : Adolescence, dismemberment anxiety, masochism, death instinct, work of melancholy.
Le psychologue, le traumatisme, l'insécurité et la gestion de l'économie psychosomatique. L'expérience d'une consultation psychologique à Alger
Authors: Aït Sidhoum Mohand ou Ahmed 
Published: April 2023

meros resultados de tal trabajo, conducido sobre pacientes habiendo consultado esencial- mente por una sintomatologia de orden mental, Uevan a pensar que los individuos que componen tal poblaciôn, alcanzan a administrar su economia psicosomâtica en tal situaciôn, sin cambio notable : se revelan particularmente resistentes. Cuando la presiôn que se ejerce sobre estos sujetos sobrepasa su capacidad de contenciôn, éstos reaccionan regular- mente a través de una exacerbaciôn de su sintomatologia inicial.
Palabras-clave : Traumatismo, Equilibrio psicosomâtico, Consulta psicolôgica, Algeria.