bluuebunny/crossref_metadata_2025_split huggingface dataset:
Analysis of genetic diversity and population structure in Cambodian melon landraces using molecular markers
Authors: Naznin Pervin Mst , Imoh Odirichi Nnennaya , Tanaka Katsunori , Sreyn Ouch , Shigita Gentaro , Sophea Yon , Sophany Sakhan , Makara Ouk , Tomooka Norihiko , Monden Yuki , Nishida Hidetaka , Kato Kenji 
Published: January 2023

Abstract
Genetic diversity of Cambodian melon was evaluated by the analysis of 12 RAPD and seven SSR markers using 62 accessions of melon landraces, and compared with 231 accessions from other areas for genetic characterization of Cambodian melon. Among 62 accessions, 56 accessions were morphologically classified as small-seed type whose seed length was shorter than 9mm as like groups Conomon and Makuwa. Gene diversity of Cambodian melon was 0.228 which was equivalent to those of groups Conomon and Makuwa and smaller than those of Vietnamese and Central Asian landraces. Phylogenetic tree constructed from genetic distance matrix classified 293 accessions into three major clusters. Small-seed type accessions from east and southeast Asia formed clusters I and II, which were distantly related with cluster III consisted of large-seed type melon from other areas. All of Cambodian melon belonged to cluster I except three accessions, along with those from Thailand, Myanmar, Yunnan (China) and “Dua thom” from northwestern part of Vietnam, indicating genetic similarity in these areas. In addition, Cambodian melon was not differentiated among geographical populations. Conomon and Makuwa were classified into cluster II, together with melon groups from plain areas of Vietnam. The presence of two groups of melon in southeast Asia was also indicated by population structure and PCO analysis. These results indicated close genetic relationship between Cambodia and the neighboring countries, and suggested that Cambodian melon is not directly related with the establishment of Conomon and Makuwa.
Age-related elevation of O-GlcNAc causes meiotic arrest and subfertility in male mice
Authors: Yao Bing , Qian Zhang , Li Chuwei , Zhao Shanmeizi , Zhang Hong , Ma Rujun , Ge Xie , Jing Jun , Chen Li , Ma Jinzhao , Zheng Lu , Zhang Kemei , He Zhaowanyue , Xue Mengqi , Lin Ying , Jueraitetibaike Kadiliya , Feng Yuming , Cao Chun , Tang Ting , Sun Shanshan , Teng Hui , Zhao Wei 
Published: January 2023

Abstract

In recent years, the postponement of childbearing has become a critical social issue. Male fertility is negatively associated with age because of testis aging. Spermatogenesis is impaired with age, but the molecular mechanism remains unknown. The dynamic posttranslational modification O-linked N-acetylglucosamine (O-GlcNAc), which is a type of monosaccharide modification, has been shown to drive the process of aging in various systems, but it has not yet been investigated in the testis and male reproductive aging. Thus, this study aims to investigate the alteration of O-GlcNAc with aging and explore the role of O-GlcNAc in spermatogenesis. Here, we demonstrate that the decline in spermatogenesis in aged mice is associated with elevation of O-GlcNAc. O-GlcNAc is specifically localized in differentiating spermatogonia and spermatocytes, indicating its crucial role in meiotic initiation and progression. Mimicking the age-related elevation of O-GlcNAc in young mice by disabling O-GlcNAcase (OGA) using the chemical inhibitor Thiamet-G can recapitulate the impairment of spermatogenesis in aged mice. Mechanistically, the elevation of O-GlcNAc in the testis leads to meiotic pachytene arrest due to defects in synapsis and recombination. Furthermore, decreasing O-GlcNAc in aged testes using an O-GlcNAc transferase (OGT) inhibitor can partially rescue the age-related impairment of spermatogenesis. Our results highlight that O-GlcNAc, as a novel posttranslational modification, participates in meiotic progression and drives the impairment of spermatogenesis during aging.
A High-throughput screening system for SARS-CoV-2 entry inhibition, syncytia formation and cell toxicity
Authors: Jancy Shine Varghese , Lupitha Santhik Subhasingh , Chandrasekharan Aneesh , Varadarajan Shankara Narayanan , Nelson-Sathi Shijulal , Prasad Roshny , Jones Sara , E Sreekumar , T.R. Santhoshkumar 
Published: January 2023

Abstract
Background: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) entry to host cell is mediated through the binding of the SARS-CoV-2 Spike protein via receptor binding domain (RBD) to human angiotensin-converting enzyme 2 (hACE2). Identifying compounds inhibiting Spike-ACE2 binding would be a promising, safe antiviral approach against COVID-19.
Methods: In the present study, we have used BSL-2 compatible replication-competent vesicular stomatitis virus (VSV) replaced glycoprotein with spike protein of SARS-CoV-2 expressing eGFP reporter system (VSV-eGFP-SARS-CoV2) in a permissive cells harboring cytotoxicity marker. The high-throughput compatible SARS-CoV-2 permissive reporter system that encompasses cells stably expressing hACE2 tagged cerulean and nuclear H2B tagged with mCherry, as a marker of nuclear condensation that also enabled imaging of fused cells among infected EGFP positive cells and could give real-time information of syncytia formation.
Results: A limited high-throughput screening identified six natural products with marked VSV-eGFP-SARS-CoV2 inhibition at non cytotoxic dose. Molecular simulation studies with positive hits in complex with wild-type spike reaffirm their potential to impede viral entry. Real-time syncytia formation assay of the molecules revealed inhibition of syncytia with Didemnin B, and delayed inhibition with other natural products such as Scillaren A, Proscillaridin, Acetoxycycloheximide indicating that the assay is a reliable platform for any image based drug screening.
Conclusion: BSL-2 compatible assay system equivalent to the infectious SARS-CoV-2 is a promising tool for high-throughput screening of large compound libraries for viral entry inhibitors against SARS-CoV-2 along with toxicity and effect on syncytia. Studies using clinical isolates of SARS-CoV-2 is warranted to confirm the antiviral potency of the leads and the utility of the screening system.
Tumor-nonspecific vaccines improve the efficacy of CD3 bispecific antibody therapy in solid tumors
Authors: Middelburg Jim , Sluijter Marjolein , Schaap Gaby , Göynük Büşra , Lloyd Katy , Ovcinnikovs Vitalijs , Zom Gijs , Marijnissen Renoud , Sandker Gerwin , Heskamp Sandra , Burg Sjoerd van den , Arakelian Tsolere , Ossendorp Ferry , Arens Ramon , Schuurman Janine , Kemper Kristel , Hall Thorbald van 
Published: January 2023

Abstract
CD3 bispecific antibody (CD3 bsAb) therapy is clinically approved for refractory hematological malignancies, but responses in solid tumors have been limited so far. One of the main hurdles in solid tumors is the lack of sufficient T-cell infiltrate. Here, we show that pre-treatment vaccinations, even when they comprised tumor-unrelated antigens, induced CXCR3-mediated T-cell influx in immunologically ‘cold’ mouse tumor models. In the absence of CD3 bsAb, the infiltrate was mainly confined to the tumor invasive margin, however subsequent CD3 bsAb administration induced profound infiltration of activated effector CD8 T cells into the tumor cell nests. This combination therapy installed a broadly inflamed Th1-type tumor microenvironment, resulting in effective tumor eradication. Interestingly, multiple vaccination strategies, including synthetic long peptides, viruses and COVID-19 mRNA, empowered CD3 bsAb therapy. Our results imply that eliciting tumor infiltration with vaccine-induced tumor non-specific T cells can greatly improve the efficacy of CD3 bsAbs in solid tumors.
Estimating prevalence of bereavement, its contribution to risk for binge drinking, and other high-risk health states in a state population survey, 2019 Georgia Behavioral Risk Factor Surveillance Survey
Authors: Miles Toni , Li Changle , Khan M Mahmud , Bayakly Rana , Carr Deborah 
Published: January 2023

Abstract
Background: This report examines the cross-sectional association between new bereavement and binge drinking. Bingeing is defined as 4 or more drinks (women) or 5 or more drinks (men) in a 2-to-4-hour setting. In the 2019 Georgia Behavioral Risk Factor Surveillance Survey (BRFSS), bereavement is a defined as a ‘Yes’ answer to ‘Have you experienced the death of a family member or close friend in the years 2018 or 2019?’.
Methods: Georgia BRFSS is a complex sampling survey administered annually. It is designed to represent the 8.1 million persons in the U.S. state of Georgia aged 18 years and older. Alcohol consumption is routinely measured in the common core. In 2019, the state added a new item probing for bereavement in the prior 24 months predating the pandemic of COVID-19. Imputation and weighting techniques were applied to yield population prevalence rates of new bereavement, bingeing, and their co-occurrence with other high-risk health states. Models, adjusted for age, gender, and race were used to estimate the risk for other unhealthy behaviors posed by the co-occurrence of bereavement and bingeing.
Results: In Georgia, bereavement is common (45.8 %), and alcohol consumption is common (48.8 %). Bereavement and alcohol use co-occurred among 1,796,817 persons (45 % of all drinkers). The subset of co-occurring bereavement and binging totaled 608,282 persons. Within this group, the highest rates of bereavement were associated with death of a friend / neighbor (30.7%) or 3 plus deaths (31.8%).
Conclusions: While bingeing is a known risk to public health, its co-occurrence with new bereavement is a new observation. Public health surveillance systems need to monitor this co-occurrence to protect both individual and societal health.
Modeling of the effect of subperiosteal hydrostatic pressure conductivity between joints on decreasing contact loads on cartilages. Floating skeleton theory
Authors: Pitkin Mark R. 
Published: January 2023

Abstract

Chronic overloading of cartilages may lead to their irreversible destruction, as seen in people with osteoarthritis. We hypothesize that overloading begins and progressing when one or more joints become isolated from the newly discovered physiological system - Floating Skeleton (FS), devoted to keeping the loads in a safe range. To demonstrate in a model experiment how subperiosteal hydrostatic pressure conductivity between joints decreases contact loads on cartilages we constructed a physical model from a commercial Ziploc bag, which walls have been glued together in its center with a small caramel plug. After pouring water in the bag, we put a standardized weight on the upper wall of the bag over the caramel plug and recorded the load transmitted to the measuring plate directly under the plug. Then, the caramel plug was dissolved so that an unobstructed water layer between the bag’s walls was restored. The same weight was placed at the same spot and the load on the measuring plate was recorded. Restoration of the water layer between the bag’s walls caused a reduction of the load. The experiments conclusively demonstrated that contact pressures can be redistributed when the conditions of the Pascal Law are met.
Identification of psycho-socio-judicial trajectories and factors associated with PTSD after recent sexual assault: the IADViSe study protocol for a multi-centric prospective study using mixed methods and artificial intelligence
Authors: Fedele Emma , Trousset Victor , Schalk Thibault , Oliero Juliette , Fovet Thomas , Lefevre Thomas 
Published: January 2023

Abstract

Background Sexual assault (SA) has numerous negative outcomes on physical, sexual and mental health, social life, financial stability and overall quality of life. It is known as one of the most traumatogenic event. However, not all people who experienced SA will develop negative functional outcomes. Multiple risk and protection factors can influence their psycho-socio-judicial trajectories. The way by which these factors impact trauma adaptation are not always clear. Guided by an ecological framework, this project aims to (1) describe psycho-socio-judicial one-year trajectories of people recently exposed to SA, to (2) identify predictive factors for the development of posttraumatic stress disorder (PTSD) at first forensic examination using artificial intelligence and to (3) explore the notions of “victim” and “trauma” and the needs and experiences of people who have been sexually assaulted. Methods and design This longitudinal multi-centric cohort study will use a mixed-method approach. Quantitative cohort data will be collected based on questionnaires repeated in time, starting from first forensic examination and covering up to a year after the SA, measuring factors associated with PTSD, mental, physical, social and overall functional outcomes, and psycho-socio-judicial trajectories. Qualitative data will be based on semi-structured interviews with members of the cohort, people who experienced SA but not included in the cohort, and actors professionally involved in their psycho-socio-judicial care. Bivariate and multivariate analyses will be performed to study the associations between each variable and mental, physical, social and judicial outcomes. Predictive analyses will be conducted on multiple prediction algorithms to predict PTSD. Qualitative data will be integrated to the quantitative data to identify psycho-socio-judicial trajectories of participants and data on the representations of the place and usefulness of the notions of “trauma” and “victims” will be used independently. Discussion This project will collect numerous data never before collected over such long periods, which will lead to unprecedented results on psycho-socio-judicial trajectories of people who experienced SA. By being based on the entire psycho-socio-judicial chain and on the perspective of people who experienced SA, this work will allow to make innovative recommendations to improve their care at all levels, from the initial forensic examination.
Mortality Related Factors in Penetrating Abdominal Trauma
Authors: Bernal Andrés Felipe , Latiff Mario Alberto , Núñez-Rocha Ricardo E. , Rodríguez Lina M , Sierra Andres Mauricio Garcia , Rey Carlos , Tabares Raquel , Alvarado Kelly , Espitia David , Conde Danny , Hernández Juan David , Girón Felipe 
Published: January 2023

Abstract
Background: Penetrating abdominal trauma is a frequent cause of death in Latinamerica with high rates of morbidity and mortality. A wide variety of therapeutic appraches have been proposed for the manegement of this pathology. In this study, we aimed to analyze the factors related to mortality and to describe outcomes of patients in penetrating abdominal trauma.
Methods: Retrospective review of a prospectively collected database was performed. All patients with penetrating abdominal, or thoracoabdominal injury that required urgent laparotomy or laparoscopic exploration between 2018-2020 in our institution were included.
Results: A total of 231 patients were included. Male patients were 92.21% and the median age was 28 IQR (22-37). 56.2% patients were ASA I, 19.9% ASA II, 4.7% ASA III, 7.3% ASA IV and 11.6% unknown. 77.5% patients had sstab wounds as penetrating abdmonal trauma etiology and 22.1% by gunshots. 76.2% of the wounds were made in the thoracoabdominal area, 73.8% were abdominal injuries and 24.2% were thoracic. 77.9% of patients presented a PATI score under 25 points. Comorbidities, gunshot wounds, ASA, need of blood product transfusion, number of pRBC needed, surgical approach and organ injury had a statistically significant association (p<0.05) with total mortality and mortalifty after 48 hours. There was also a statistically significant association between comorbidities (OR 407.405 [1.4-118534.05 95% IC]), ASA III (OR 0.004 [0-0.644 95% IC]), and blood product transfusion (OR 0 [0-0.877 95% IC]) with overall mortality.
Conclusion: PATI scale appear to be a feasible tool to predict worst outcomes in abdominal penetrating trauma patients. Residents as first surgeons showed no evidence of a higher complications rate.
Industrial Wastewater Irrigation Increased Higher Heavy Metals uptake and Expansins, Metacaspases, Cystatin Genes Expression in Parthenium and Maize
Authors: Penzy Kinza , Muhammad Said , Shahzad Muhammad , Hussain Imran , Khan Sabaz Ali , Abbasi Arshad Mehmood , Khan Imtiaz , Ahmad Rafiq 
Published: January 2023

Abstract

Industrial wastewater irrigation of agriculture crops could cause a lot of environmental and health problems in developing countries due to heavy metals deposition in agriculture soils as well as edible plants consumption by human beings. Therefore, this study was conducted to find out heavy metals’ concentration in industrial wastewater and soil irrigated with that wastewater. In addition, the impact of industrial wastewater irrigation on Parthenium hysterophorus and Zea mays genes involved in growth improvement and inhibition of selected plants. For this purpose, plant samples from agriculture fields irrigated with wastewater from Hattar Industrial Estate (HIE) of Haripur, Pakistan and control plants from non-contaminated soil irrigated with tape water were collected after fifteen and forty-five days of germination. Heavy metals concentration in the collected plant samples, wastewater and soil were determined and results revealed that the study area was predominantly contaminated with Cr, Pb, Ni, Cu, Co, Zn and Cd concentrations of 38.98, 21.14, 46.01, 155.73, 12.50, 68.50 and Cd 7.01 mg/kg, respectively. The concentrations of these heavy metal have surpassed permissible limit of these metals in normal agriculture soil. Expansins and cystatin (plant growth enhancers), metacaspases (plant growth inhibitor) genes expression were studied through reverse transcription polymerase chain reaction and results showed that the expression of these genes was higher in samples collected from wastewater-irrigated soils as compared to control. More expression of these genes was observed in 45 days old samples as compared to 15 days old samples and control. Taken together, this study suggests the use of Parthenium and maize for phytoremediation, however, they should not be used for eating purposes if irrigated with industrial wastewater.
Mitochondrial Aconitase enzymatic activity: a potential long survival biomarker in the blood of ALS patients
Authors: González-Mingot Cristina , Miana Francisco Javier , Iñarrea Pedro , Iñíguez Cristina , Capablo JoseLuis , Osta Rosario , Gil-Sánchez Anna , Peralta Silvia , Brieva Luis , Larrode Pilar 
Published: January 2023

Abstract
Background: Amyotrophic Lateral Sclerosis (ALS) is a multisystemic, progressive, neurodegenerative disorder. Despite it being generally fatal within a period of 2–4 years, it is highly heterogeneous; as a result, survival periods may vary greatly among individual patients. In the absence of a single reliable test for ALS, Biomarkers can serve as tools for diagnosis, prognosis, indicators of therapeutic response, and future therapeutics. Free radical-dependent mitochondrial damage is believed to play a crucial role in neurodegeneration in ALS. Mitochondrial Aconitase, which is also known as Aconitase 2 (Aco2), is a key Krebs cycle enzyme and is involved in the regulation of cellular metabolism and iron homeostasis. Aco2 is very sensitive to oxidative inactivation and can aggregate and accumulate in the mitochondrial matrix, causing mitochondrial dysfunction. A loss of Aco2 activity may therefore reflect increased levels of mitochondrial dysfunction due to oxidative damage and could be relevant to ALS pathogenesis.
The aim of our study was to confirm changes in Mitochondrial Aconitase activity in peripheral blood and to determine whether such changes are dependent on, or independent of, the patient's condition and to explore the feasibility of using them as valid biomarkers for quantifying disease progression and as an individual prognosispredictor in ALS.
Methods: We measured Aco2 enzymatic activity in platelets of blood samples taken from 22 controls and 26 ALS patients at different stages of disease development. We then correlated antioxidant activity with clinical and prognostic variables.
Results: Aco2 activity was significantly lower in the 26 ALS patients than in the 22 controls (p<0.05). Patients with higher levels of Aco2 activity survived longer than those with lower levels (p<0.05). Aco2 activity was also higher in patients with earlier onsets (p< 0.05) and in those with predominantly upper motor neuron signs.
Conclusions: Aco2 activity is an independent factor that can be used in the long-survival prognosis of ALS. Our findings suggest that blood Aco2 is a leading candidate for use as a biomarker for improving prognosis. It could facilitate participant stratification in future ALS therapeutic trials and offer additional potential for therapeutic targeting.
Efficacy of adipose-derived stem cells combined with different scaffolds to repair bone defects: a systematic review and Meta-analysis
Authors: XIONG YB , LIU JH , HE YH , LI CL 
Published: January 2023

Abstract
At the present stage, the most traditional techniques for the treatment of bone defects often fail to achieve the desired results. The tissue engineering technology about bone regeneration emerged as a new method for treating bone defects. Cells and scaffolds are the two most important key factors in tissue engineering, and there are many types of scaffolds are already in use in experiments. And there are so many studies are now on different Cellular scaffolds filled with ADSCs (adipose-derived stem cells). But these researches are still in the animal experimental stage. This study informed the clinical application of ADSCs through an evidence-based medicine analysis to evaluate the efficacy of ADSCs in repairing the bone defect. We systematically searched databases, collected relevant studies, and screened them, then analyzed the data using specialized tool and rated the credibility of the results at the end. The final results concluded that the efficiency of treatment of ADSCs combined with scaffold treatment group was superior to the control group. Quality of evidence (GRADE): high. The combination of ADSCs with cellular scaffolds may be effective in promoting repair of bone defects. However, additional, more demanding experiments are still needed to test the effect of ADSCs on bone regeneration.