bluuebunny/crossref_metadata_2025_split huggingface dataset:
Morphological bases of reorganization of the hippocampal interneuronal relationships in white rats after severe traumatic brain injury
Authors: Akulinin V. A. , Shoronova A. Yu. , Stepanov S. S. , Korzhuk M. S. , Makar'eva L. M. , Tsuskman I. G. , Avdeev D. B. , Stepanova L. V. 
Published: July 2023

The aim of the study was to investigate morphological changes in neurons, glia and synaptic terminals in cytoarchitectonic fields CA1 and CA3 of the hippocampus in white rats after severe traumatic brain injury (STBI). Material and methods. This experimental study involved outbred adult white Wistar rats. STBI (main group, n = 30) was simulated under anesthesia (Zoletil-100) on the tailor-made device. Intact animals (n = 6) were used as controls. Morphological assessment of the hippocampal nervous tissue (in fields CA1 and CA3) was carried out using light microscopy (staining with hematoxylin and eosin, thionin according to Nissl), immunohistochemical reactions for synaptophysin (p38), Caspase 3, GFAP, and morphometric investigation techniques (ImageJ 1.53 program) in animals of the control group and in experimental animals in 1, 3, 7, 14, 30 days after STBI. Statistically hypotheses were tested using nonparametric tests (Kolmogorov–Smirnov, Kruskal–Wallis, Mann–Whitney, Wilcoxon, Spearman tests) in the Statistica program. 10.0. Results. In a day after STBI, there were noted degeneratively altered dark neurons, shadow cells, small foci of neuron prolapse, pronounced manifestations of edema-swelling in the perikarya and neuropil. Coagulative-ischemic mechanisms of neuronal death prevailed, structural and immunohistochemical signs of apoptosis activation (Caspase 3 in the perikarya) were detected in sporadic cells of the field CA1. In the late period of the study (in 30 days), hydropic changes, chromatolysis with cytoplasmic clearing, hyperchromia without and with homogenization of neuronal structures persisted. An increased neuroglial index was distinctive. Statistically significant differences in the main morphometric parameters were revealed between segments and terms. The total number density of neurons decreased by 43.8 % in CA1 and in CA3 by 22.0 % in 30 days after injury; a significant (20–40 %) deficit of neuropil synapse terminals remained. Thus, the compensatory-restorative reorganization of the hippocampal neuronal networks occurred combined with its structural and functional insufficiency. Probably, Caspase 3 was crucial in implementing neuroplasticity. Conclusion. Post-STBI diffuse-focal pathomorphological changes were revealed in CA1 and CA3 cells of the hippocampus. An increased relative content of glial cells indicated the realization of sanogenesis of the nervous tissue. Reorganization of interneuronal connections occurred during 30 days of observation, caspases might be participants of this process.
Epithelial-mesenchymal transition: the history of the concept, debatable issues
Authors: Shevlyuk N. N. 
Published: July 2023

The paper discusses diverse aspects of the concept of epithelial-mesenchymal transition and compares the basics of this concept with the classical concepts of tissue biology. The idea of the epithelial-mesenchymal transition was first suggested by Elizabeth Hay (1927–2007) in 1968 based on the analysis of tridermogenesis in the development and growth of the avian embryo. In the late 80s – early 90s of the twentieth century, the concept of epithelial–mesenchymal transition won supporters-pathomorphologists in our country who applied the concept to explain mechanisms of multiple pathological processes. The controversial issue arises: to which extent the concept of epithelial-mesenchymal transition is validated. It should be noted that a number of its basic principles are open to question. Thus, it is hardly correct to consider the cells of the ectoderma as epithelial cells, since they do not express immunohistochemical markers of epithelial tissue cells. That is, cells with a true epithelial phenotype are not yet represented at this stage of embryogenesis. It should also be taken into account that all evidence for the epithelial-mesenchymal transition is based on indirect immunocytochemical findings (decreased expression of epithelial markers, increased expression of markers of mesenchymal tissue genesis). Moreover, saying about the “epithelial-mesenchymal transition” specialists mean cell populations and not specific cells, which is hardly the same. All the above allows arguing the existence of the epithelial-mesenchymal transition; this issue needs additional research and more fact-based support.
An uncommon case of placental histopathology with antenatal fetal death under new coronavirus infection
Authors: Kasparova A. E. , Khadieva E. D. , Kutefa E. I. , Yanin V. L. , Vasil'kovskaya E. N. , Chegus L. A. , Sazonova N. A. , Khidirnebieva F. R. 
Published: July 2023

Since the beginning of the COVID-19 pandemic, there have been a number of studies related to the impact of SARS-CoV-2 on the course of pregnancy and fetus condition. As observed, the placentas of women who have had a new coronavirus infection often contain more villous agglutination and subchorionic thrombi than placentas in SARS-CoV-2-negative women. To date, several isolated publications have reported clinical cases of fetal death in mothers infected with coronavirus infection. The authors have made an assumption on the association of adverse outcomes with placental lesions. The aim of the study was to analyse a clinical case of a moderate-course new coronavirus infection in a pregnant woman at a long gestation period who underwent an antenatal fetal death, and evaluate the features of placental histopathology and their impact on adverse gestational outcomes. Material and methods. The authors have analysed Russian and international research publications from various sources, including eLIBRARY.RU, CyberLeninka, PubMed databases etc. and, considering the data obtained, investigated a clinical case of intrauterine fetal death in a pregnant woman infected with SARS-CoV-2. The placenta was studied in accordance with the clinical recommendations of the Russian Society of Pathologists "Rules for placental pathological and anatomical examination" and the international classification of placental lesions (Amsterdam, 2015). Results. The results obtained support the association between acute diseases of the upper respiratory tract and developing severe hemodynamic disorders in the "mother-placenta-fetus" system in pregnant women infected with SARS-CoV-2. Systemic inflammation associated with new coronavirus infection appears to be one of the mechanisms for developing placental disorders.
Antimicrobial Activity and Wound Healing Properties of Aloe Arborescens Extract: An in Vivo Study
Authors: Ghane Fatemeh , Rasouli Pegah , Khanjari Benafshe , Yousofi Alireza , Zarenezhad Ali , Fattahi Hossein , Rostami Chijan Mahsa , Ghasemian Abdolmajid , Miladpour Behnoosh , Zarenezhad Elham 
Published: July 2023

Background & Objective: Burn is one of the prominent causes of death around the world, however drug discovery attempts for burn healing has not been entirely successful. Aloe arborescens (A. arborescens), is effective in the burning wounds healing and growth inhibition of bacterial pathogens. Our objective was to assess the wound healing and antibacterial effects of A. arborescens in vivo.
Materials & Methods: Thirty healthy Wistar rat animals were enrolled. The treatment process continued for 21 days and sampling was conducted on days 14 and 21 and the tissue slides were sent to the pathology laboratory for testing. The bactericidal activity of A. arborescens extract was evaluated using the disc diffusion method.
Results: A. arborescens demonstrated a significant effect on the healing of burn wounds. Furthermore, the antibacterial effects of the A. arborescens extract against Gram-negative (Escherichia coli, Pseudomonas aeruginosa) was significantly higher than that against Grampositive (Staphylococcus aureus, Bacillus cereus) bacterial species.
Conclusion: In conclusion, this study indicated that A. arborescens extract had an improving effect on the healing process of third degree burns without toxicity to the tissue.
Green Synthesis of Au-doped SnO 2 Nanoparticles Using Teucrium Polium Plant Extract for the Evaluation of Their Physicochemical and Antibacterial Properties
Authors: Karimi Merat , Sadeghi Ehsan , Zahedifar Mostafa 
Published: July 2023

Background & Objective: The biological production of nanoparticles (NPs) is a technique that has garnered a lot of interest because it is inexpensive, straightforward, and friendly to the natural environment. This study explores the green synthesis of Au-doped SnO 2 NPs using Teucrium polium plant extract and evaluates their antimicrobial properties in comparison with two commonly used antibiotics.
Materials & Methods: Initially, an extract of Teucrium polium was made using water, and then it was combined with solutions of tin (II) chloride dehydrate [SnCl 2 .2H 2 O] and gold (III) chloride trihydrate [HAuCl 4 .3H 2 O]. The crystal structure was studied using X-ray diffraction (XRD). The form, structure, and grain size of the NPs were analyzed using scanning electron microscope (SEM) and transmission electron microscope (TEM). The synthesized NPs were tested for their antibacterial properties.
Results: The XRD and EDS analyses revealed that the SnO 2 : Au phase formed an average crystallite size of 22 nm and an Au dopant content of around 2%. The SEM and TEM investigations demonstrated that the NPs were formed in a regular and nanoscale manner, with diameters ranging from 25 to 30 nm. MIC and MBC of SnO 2 : Au NPs against the studied bacteria (S.aureus ATCC 43300, P.aeruginosa PAO1) were reported 9.6±0.13- 9.6±0.07 μg/mL and 25.9±11.5- 32.5±11.2 μg/mL.
Conclusion: Green-synthesized NPs exhibit significant antimicrobial activity against both Gram-positive and Gram-negative bacteria, even at low concentrations. The NPs effectively eliminate bacteria and prevent biofilm formation.
High Frequency of HPV Genotypes 16 and 18 Found in Breast Cancer Patients: Evidence for a More Comprehensive HPV Vaccination Program in Iran
Authors: Khalilian Mohaddeseh , Hosseini Seyed Masoud , Vahidi Emami Hassan , Madadgar Omid 
Published: July 2023

Background & Objectives: Papillomaviruses are found in many different types of infections and in a wide range of animals and humans. They can cause health problems, including benign and malignant tumors. In the present study, the association between human papillomavirus (HPV) infection and breast cancer (BC) in Iran was investigated.
Materials & Methods: In this cross-sectional study, the presence of the HPV genome was investigated in BC-suspected tissues for the first time in Qom Province, Iran. A total of 400 samples (including 200 BC-suspected tissue samples and 200 blood samples of women without BC) were collected from women referred to two cancer-specific general hospitals. To determine the presence of the L1 gene of HPV in the collected samples, nested polymerase chain reaction (PCR) was performed. Then, HPV-positive samples were tested by PCR using high-risk specific HPV-16 and 18 primers.
Results: Out of 200 BC-suspected tissue samples, 172 were malignant (in terms of pathology). Based on the nested PCR method, the L1 gene of HPV was detected in 12% (24/200) of the BC-suspected tissue samples and in 1.5% (3/200) of the blood samples from women without BC. The high-risk HPV genotypes (which were the predominant types) were present in 75% of the samples.
Conclusion: The results of the current study show a high frequency of HPV-16 and 18 genotypes in human BC in Iranian women. This is almost certainly due to poor rates of HPV vaccination, and it is strongly recommended that health organizations (such as the World Health Organization [WHO]) ensure adequate coverage of highly effective HPV vaccination in Iran.
Radioprotective Effects of Melatonin on Gamma Radiation- Induced Tissue Damage in Rats
Authors: Bagheri Gholam Reza , Ghaffari Hamid Reza , Valizadeh Majid , Jahantigh Hossein Ali , Poursamimi Marzieh , Shahriari Hosein , Poursamimi Javad 
Published: July 2023

Background & Objective: Gamma rays are widely used in medicine despite their harmful effects on health. Our study evaluated the protective effects of melatonin on kidney, heart, and liver tissues.

Materials & Methods: Seventy-two adult male Wistar rats were categorized into nine groups. Groups 2 and 3 only received whole-body γ-ray irradiation (WBI) (2.0 Gy), no melatonin, interval time (IT) 8hr and 24hr. Groups 4 and 5 received WBI (8.0 Gy, no melatonin, 8hr and 24hr). Groups 6 and 7 received melatonin at 60 minutes (min) before WBI(2.0 Gy). Groups 8 and 9 received melatonin before WBI(8.0 Gy). All the rats were sacrificed 8 or 24 h after the experiments for laboratory and histopathological analysis. Serum levels of Blood Urea Nitrogen(BUN), Creatinine (Cr), Lactate dehydrogenase (LDH), Potassium (K), Gamma-glutamyl Transferase (GGT), Serum Glutamic-Oxaloacetic Transaminase(SGOT), Serum Glutamic-Pyruvic Transaminase(SGPT), Alkaline Phosphatase(ALP), c-reactive protein(CRP), Troponine(TPO) and histological features of liver, heart and kidney tissues were assessed. Statistical analysis was performed by One-Way ANOVA.

Results: Our data did not indicate significant differences in BUN, Cr, K, and CRP between groups with or without melatonin treatment (P>0.05); but differences were significant for LDH, GGT, SGOT, SGPT, ALP, and TPO (P<0.05). The results showed that radiation-induced histopathological effects on the liver, heart, and kidneys were mitigated in the groups six to nine.

Conclusion: The existence of significant differences in serum levels of LDH, GGT, SGOT, SGPT, ALP, and TPO in groups(6 to 9) and amelioration of the histopathological effects of irradiation on the liver, heart, and kidneys in the groups six to nine showed that the melatonin(100 mg/kg) is able to protect the body in gamma-radiation(2.0 Gy and 8.0 Gy).
Investigation of Lupus Nephritis Cases with Special Reference to Activity and Chronicity Indices
Authors: Farshid Khashayar , Noroozinia Farahnaz , Pourjabali Masoumeh 
Published: July 2023

Background & Objectives: The International Society of Nephrology/ Renal Pathology Society (ISN/RPS) classification is based upon criteria which differentiate acute and chronic phases of Lupus Nephritis. Activity/Chronicity Index grants us a higher insight on the level of pathological lesions and treatment outcome by examining the reversibility of lesions .The present investigation was done in order to highlight the importance of activity and chronicity indices in the course of Lupus Nephritis.Materials & Methods: Seventy-three Kidney biopsy samples of Lupus Nephritis patients were examined. The information was recorded in a check-list and was then statistically analyzed.Results: Lupus Nephritis is importantly age-related, frequency of Lupus Nephritis increases with age until the age of 40 years; 21–40 years being the most frequent among studied patients. A prominent decline was seen after the age of 40. Regardless of age, the occurrence was more frequent in females. Class IV was most frequent in all ages and in both sexes. Both Activity and Chronicity scores were slightly higher in females. Activity Index was higher in ages of 11 to 30 years, whereas Chronicity Index was highest in 41 to 50 years of age. The highest Activity Index was reported in Class IV while the highest Chronicity Index was reported in Class V. The least Activity Index was shown in Class VI while Classes II and I had the lowest Chronicity Index. Endocapillary hypercellularity was the most frequent active lesion and tubular atrophy was the most frequent chronic lesion. It is noteworthy that fibrotic crescents were significantly less common among chronic lesions.Conclusion: Histopathological findings, clinical and para-clinical data could furnish more information on disease process, treatment, quality of life and mortality rate.
Evaluating the Effect of Telenursing and Multimedia Self-Care Educational Program on the Level of Existential Anxiety in Patients with COVID-19: A Quasi-Experimental Study
Authors: Khoddami Asma , Imani Elham , Jamhiry Reza , Hoseini Teshnizi Saeed 
Published: July 2023

Background & Objective: For patients, COVID-19 disease is a reminder of non-existence and death. For this reason, these patients experience existential anxiety. One of the effective factors in the control and treatment of this disease is to educate patients on self-care to reduce the burden of these psychological problems on them. The present study was designed and conducted with the aim of evaluating the effect of self-care educational program using telemedicine and multimedia methods on the level of existential anxiety in COVID-19 patients.Materials & Methods: This study was a quasi-experimental research conducted on 88 COVID-19 patients referred to the comprehensive health service centers of Bandar Abbas, Iran from 2020 to 2021. Th0ey were selected using a convenience sampling method. The samples were randomly assigned to the multimedia (44 patients) and telenursing (44 patients) groups. Patients in both groups received education on self-care during COVID-19 disease for 21 days. The Existence Anxiety Scale was completed before and immediately after the intervention. Data were analyzed with SPSS software version 26. Descriptive statistics, normality test, independent t-test, homogeneity of variance and covariance, univariate and multivariate analysis of covariance were used.Results: The mean score of existential anxiety at the beginning of the study was 90.50± 12.57 and 85.25 ±16.12, respectively, in the telenursing and multimedia groups, and after the intervention, it was 46.88 ± 6.38 and 65.40 ± 9.59, respectively, in the telenursing and multimedia groups. After the intervention, the existential anxiety score was significantly reduced in the telenursing group compared to the multimedia group (p <0.001).Conclusions: Based on the findings of this study, self-care education programs and knowledge about necessary care during COVID-19 illness should be considered for patients in comprehensive health centers, and telenursing should be used for more effective self-care education.
Graphite Furnace Atomic Absorption Spectrometry for Biomonitoring of Blood Lead Level in Quality Control Blood Materials
Authors: Aliasgharpour Mehri 
Published: July 2023

Background & Objective: Lead (Pb) is a major toxicant that has threatened human health for years. There is no safe level of exposure and deposition of a small amount of Pb in the human body has a negative impact on an individual's health. Despite recent reductions in its use, it has caused extensive environmental contamination as well. In the present study,the intralaboratory quality control assessment is reported as an initial step in lead determination by Graphite furnace atomic absorption spectrometry (GFAAS).
Materials & Methods: Varian SpectrAA-220 with partition tube and deuterium background correction was used for the analysis. The evaluation quality control blood material was Seronorm trace elements whole blood (levels I &II).
Results: The lowest method limit of detection was 0.74 μg/dL, and based on the regression analysis, the lowest quantification concentration was 3.00 μg/dL (CV = 4.6 %). In addition, obtained %CV for Seronorm (I) & Seronorm (II) was 7.3% & 5.4% respectively.
Conclusion: In recent years, several diverse technological advancements in blood lead level determination have occurred. At the same time, demands for blood lead (BPb) level determination by GFAAS has been increased by public health in order to identify children exposed to lead in the environment, and by occupational health to reduce excessive exposure in the lead industries. The obtained results indicated that for BPb analysis, the method described by GFAAS is still a reference routine analytical technique that requires less time and no matrix matched standard curve, allowing blood samples to be run against aqueous calibration standards which eliminates the need for standard additions.
PENGEMBANGAN PROGRAM TAKHASUS AL-QURAN DI PONDOK PESANTREN KRAPYAK YOGYAKARTA
Authors: Rosita Neni 
Published: July 2023

Pondok pesantren merupakan lembaga pendidikan Al-Qur’an yang memiliki kecenderungan membangun sebuah relasi suci antara kiai dan santri. Aktivitas menghafal Al-Qur’an yang dilakukan secara berkelompok dapat menumbuhkan ikatan emosional yang kuat, karena menghafalkan Al-Qur’an memerlukan waktu yang lama. Pengajian Al-Qur’an berlangsung cukup instens, sehingga membentuk pribadi yang bermoral dan kesadaran sosial yang melekat dalam kepribadian seorang penghafal Al-Qur’an. Sebagai respon positif dari pesantren terhadap perubahan dan kemajuan zaman dalam modernisasi sistem pendidikan, maka pondok pesantren telah memulai melakukan perubahan dan penyesuaian, baik menyangkut tujuan pendidikan, kurikulum, metode pengajaran, sarana pendidikan. Terjadinya modernisasi dalam sistem pendidikan pesantren bukan berarti bahwa semua elemen-elemen atau unsur-unsur yang menjadi ciri khusus dari sebuah pesantren tersebut mengalami perubahan dibuang atau dihilangkan. Orientasi pendidikan di Pondok Pesantren Ribathul Qur’an wal Qira’at yaitu mementingkan masa depan para santrinya setelah dapat menyelesaikan hafalan dari pondok dengan tujuan menjadi insan Qur’ani.
Challenges of Implementing a Sustainable Corporate Social Responsibility Program PT. Kalimantan Prima Persada
Authors: Sucipto Adi 
Published: July 2023

PT Kalimantan Prima Persada (PT KPP) is a one of mining industry services in Kutai Kartanegara Regency which operates in three villages. The practice of Corporate Social Responsibility in these three villages has used to giving cash. Nowadays, PT KPP seeks to change the existing pattern into a planned program. In line with this, this study aims to obtain an overview of CSR practices and challenges in order to optimize the use of PT KPP's CSR funds. The method used in this research is descriptive with a qualitative approach. Based on the conditions, three main problems were found. First, lack of controlling from PT KPP in planning process. Second, low capacity of local human resouces so CSR funds are mostly used for social and infrastructure sectors. Third, economic independence program has not become a priority. Thris research suggest to enhance the programs that focus on improving community skills.
AKTUALISASI NILAI-NILAI KEARIFAN LOKAL MASYARAKAT SASAK DALAM MENGATASI MASALAH KESEHATAN DI ERA PANDEMI COVID
Authors: Aminah Siti 
Published: July 2023

Abstrak: Pada masyarakat Sasak, kearifan lokal masih dianggap sebagai suatu kekayaan yang mengandung nilai, pandangan, kebijakan dan kearifan hidup masyarakat dalam berbagai ragam permasalahan. Keterlibatan kearifan lokal dapat dilihat dalam beberapa perannya dalam sistem, pertanian, pernikahan, adat istiadat dan masalah kesehatan. Terutama di era pandemi covid-19 masalah kesehatan menjadi perhatian utama seluruh bangsa di dunia termasuk di indonesia.
Tujuan penelitian ini adalah untuk mengetahui dan mendeskripsikan masalah kesehatan yang dihadapi masyarakat sasak di era pandemi. Serta mengungkap peran serta nilai kearifan lokal masyarakat sasak dalam mengatasi masalah kesehatan di era pandemi.
Masalah kesehatan yang dihadapi masyarakat sasak umumnya sama dengan fenomena yang terjadi di tengah masyarakat serta dengan gejala yang sama. Akan tetapi hambatan penyebaran dan minimnya suspek covid-19 di Desa Sengkerang terjadi karena beberapa faktor diantaranya: 1. sistem matapencaharian, 2. Psiko-Sisial. kearifan lokal masyarakat sasak pada dua faktor di atas mengindikasikan dapat meningkatkan imunitas tubuh di era pandemi.