bluuebunny/crossref_metadata_2025_split huggingface dataset:
Nanoprojectile Secondary Ion Mass Spectrometry Enables Multiplexed Analysis of Individual Hepatic Extracellular Vesicles
Authors: Lee Seonhwa , Verkhoturov Dmitriy S. , Eller Michael J. , Verkhoturov Stanislav V. , Shaw Michael A. , Gwon Kihak , Kim Yohan , Lucien Fabrice , Malhi Harmeet , Revzin Alexander , Schweikert Emile A. 
Published: August 2023

ABSTRACTExtracellular vesicles (EVs) are nanoscale lipid bilayer particles secreted by cells. EVs may carry markers of the tissue of origin and its disease state which makes them incredibly promising for disease diagnosis and surveillance. While the armamentarium of EV analysis technologies is rapidly expanding, there remains a strong need for multiparametric analysis with single EV resolution. Nanoprojectile (NP) secondary ion mass spectrometry (NP-SIMS) relies on bombarding a substrate of interest with individual gold NPs resolved in time and space. Each projectile creates an impact crater of 10−20 nm in diameter while molecules emitted from each impact are mass analyzed and recorded as individual mass spectra. We demonstrate the utility of NP-SIMS for analysis of single EVs derived from normal liver cells (hepatocytes) and liver cancer cells. EVs were captured on antibody (Ab)-functionalized gold substrate then labeled with Abs carrying lanthanide (Ln) MS tags (Ab@Ln). These tags targeted four markers selected for identifying all EVs, and specific to hepatocytes or liver cancer. NP-SIMS was used to detect Ab@Ln-tags co-localized on the same EV and to construct scatter plots of surface marker expression for thousands of EVs with the capability of categorizing individual EVs. Additionally, NP-SIMS revealed information about the chemical nano-environment where targeted moieties co-localized. Our approach allowed analysis of population heterogeneity with single EV resolution and distinguishing between hepatocyte and liver cancer EVs based on surface marker expression. NP-SIMS holds considerable promise for multiplexed analysis of single EVs and may become a valuable tool for identifying and validating EV biomarkers of cancer and other diseases.
Beta- and gamma-band oscillatory connectivity support naturalistic reading of continuous text
Authors: Kujala Jan , Mäkelä Sasu , Ojala Pauliina , Hyönä Jukka , Salmelin Riitta 
Published: August 2023

AbstractLarge-scale integration of information across cortical structures, building on neural connectivity, has been proposed to be a key element in supporting human cognitive processing. In electrophysiological neuroimaging studies of reading, quantification of neural interactions has been limited to the level of isolated words or sentences due to artefacts induced by eye movements. Here, we combined magnetoencephalography recording with advanced artefact rejection tools to investigate both cortico-cortical coherence and directed neural interactions during naturalistic reading of full-page texts. Our results show that reading vs. visual scanning of text was associated with wide-spread increases of cortico-cortical coherence in the beta- and gamma-bands. We further show that the reading task was linked with increased directed neural interactions compared to the scanning task across a sparse set of connections within a wide range of frequencies. Together, the results demonstrate that neural connectivity flexibly builds on different frequency bands to support continuous natural reading.
Sphingosine 1-phosphate receptor 1 inhibition induces a pro-apoptotic signaling cascade in T cells
Authors: Dixit Dhaval , Hallisey Victoria M. , Zhu Ethan Y.S. , Okuniewska Martyna , Cadwell Ken , Chipuk Jerry E. , Axelrad Jordan E. , Schwab Susan R. 
Published: August 2023

AbstractEffective immunity requires a large, diverse naïve T cell repertoire circulating among lymphoid organs in search of antigen. Sphingosine 1-phosphate (S1P) and its receptor S1PR1 contribute by both directing T cell migration and supporting T cell survival. Here, we address how S1P enables T cell survival, and the implications for patients treated with S1PR1 antagonists. Contrary to expectations, we found that S1PR1 limits apoptosis by maintaining the appropriate balance of BCL2 family members via restraint of JNK activity. Interestingly, the same residues of S1PR1 that enable receptor internalization are required to prevent this pro-apoptotic cascade. Findings in mice were recapitulated in ulcerative colitis patients treated with the S1PR1 antagonist ozanimod, and the loss of naïve T cells limited B cell responses. Our findings highlight an unexpected effect of S1PR1 antagonists on the ability to mount immune responses within lymph nodes, beyond their effect on lymph node egress, and suggest both limitations and novel uses of this important class of drugs.
Cognitive limits of larvalDrosophila: Testing for conditioned inhibition, sensory preconditioning and second-order conditioning
Authors: Sen Edanur , El-Keredy Amira , Jacob Nina , Mancini Nino , Asnaz Gülüm , Widmann Annekathrin , Gerber Bertram , Thoener Juliane 
Published: August 2023

AbstractDrosophilalarvae are an established model system for studying the mechanisms of innate and simple forms of learned behaviour. They have about 10 times fewer neurons than adult flies, and it was the low total number of their neurons that allowed for an electron microscopic reconstruction of their brain at synaptic resolution. Regarding the mushroom body, a central brain structure for associative learning in insects, it turned out that more than half of the classes of synaptic connection had previously escaped attention. Understanding the function of these circuit motifs, subsequently confirmed in adult flies, is an important current research topic. In this context, we test larvalDrosophilafor their cognitive abilities in three tasks that are characteristically more complex than those previously studied. Our data provide evidence for (i) conditioned inhibition, as has previously been reported for adult flies and honeybees. Unlike what is described for adult flies and honeybees, however, our data do not provide evidence for (ii) sensory preconditioning or (iii) second-order conditioning inDrosophilalarvae. We discuss methodological features of our experiments as well as four specific aspects of the organisation of the larval brain that may explain why these two forms of learning are observed in honeybees and adult flies, but not in larvalDrosophila.
Epiphytic and endophytic microbiome of the seagrassZostera marina: Do they contribute to pathogen reduction in seawater?
Authors: Tasdemir Deniz , Scarpato Silvia , Utermann-Thüsing Caroline , Jensen Timo , Blümel Martina , Wenzel-Storjohann Arlette , Welsch Claudia , Echelmeyer Vivien Anne 
Published: August 2023

AbstractSeagrass ecosystems provide crucial ecosystem services for coastal environments and were shown to reduce the abundance of pathogens linked to infections in humans and marine organisms. Among several potential drivers, seagrass phenolics released into seawater have been suggested to play role in pathogen suppression, but the potential involvement of the seagrass microbiome in such effect has not been studied. Here we hypothesized that the microbiome of the eelgrassZostera marina, especially the leaf epiphytes that are at direct interface between the seagrass host and surrounding seawater, inhibit such pathogenic microorganisms, hence, contribute to their suppression. Using a culture-dependent approach, we isolated 88 bacteria and fungi associated with the surfaces and inner tissues of the eelgrass leaves (healthy and decaying) and the roots, plus 19 strains from surrounding seawater and sediment. We first assessed the broad-spectrum antibiotic activity of microbial extracts against a large panel of common aquatic, human (fecal) and plant pathogens, and finally mined the metabolome of 88 most active extracts. The healthy leaf epibiotic bacteria, particularlyStreptomycessp. strain 131, displayed broad-spectrum and potent antibiotic activity superior to some control drugs. Gram-negative bacteria abundant on healthy leaf surfaces, and few endosphere-associated bacteria and fungi also showed remarkable antimicrobial activity. UPLC-MS/MS-based massive untargeted metabolomics analyses showed the rich specialized metabolite repertoire of strains with low annotation rates, indicating the presence of many undescribed antimicrobials in the extracts. This study contributes to our current understanding on microbial and chemical ecology of seagrasses, implying potential involvement of the seagrass microbiome, especially the leaf epiphytes, in reduction of pathogen load in seawater. Such antibiotic activity is not only beneficial for the health of ocean, human and aquaculture sector, especially in the context of climate change that is expected to exacerbate all infectious diseases, but may also assist seagrass conservation and management strategies.
Scavenger receptor class B type I is required for efficient glucose uptake and metabolic homeostasis in adipocytes
Authors: Knaack Darcy A. , Chang Jackie , Thomas Michael J. , Sorci-Thomas Mary G. , Chen Yiliang , Sahoo Daisy 
Published: August 2023

AbstractObesity is a worldwide epidemic and places individuals at a higher risk for developing comorbidities that include cardiovascular disease and type 2 diabetes. Adipose tissue contains adipocytes that are responsible for lipid metabolism and reducing misdirected lipid storage. Adipocytes facilitate this process through insulin-mediated uptake of glucose and its subsequent metabolism into triglycerides for storage. During obesity, adipocytes become insulin resistant and have a reduced ability to mediate glucose import, thus resulting in whole-body metabolic dysfunction. Scavenger receptor class B type I (SR-BI) has been implicated in glucose uptake in skeletal muscle and adipocytes via its native ligands, apolipoprotein A-1 and high-density lipoproteins. Further, SR-BI translocation to the cell surface in adipocytes is sensitive to insulin stimulation. Using adipocytes differentiated from ear mesenchymal stem cells isolated from wild-type and SR-BI knockout (SR-BI-/-) mice as our model system, we tested the hypothesis that SR-BI is required for insulin-mediated glucose uptake and regulation of energy balance in adipocytes. We demonstrated that loss of SR-BI in adipocytes resulted in inefficient glucose uptake regardless of cell surface expression levels of glucose transporter 4 compared to WT adipocytes. We also observed reduced glycolytic capacity, increased lipid biosynthesis, and dysregulated expression of lipid metabolism genes in SR-BI-/-adipocytes compared to WT adipocytes. These results partially support our hypothesis and suggest a novel role for SR-BI in glucose uptake and metabolic homeostasis in adipocytes.
Enhanced functionalities of immune cells separated by microfludic lattice: assessment based on holotomography
Authors: Lee Mahn Jae , Kim Byungyeon , Lee Dohyeon , Kim Geon , Jung Yoonjae , Shin Hee Sik , Choi Sungyong , Park YongKeun 
Published: August 2023

AbstractThe isolation of white blood cells (WBCs) from whole blood constitutes a pivotal process for immunological studies, diagnosis of hematologic disorders, and the facilitation of immunotherapy. Despite the ubiquity of density gradient centrifugation in WBC isolation, its influence on WBC functionality remains inadequately understood. This research employs holotomography to explore the effects of two distinct WBC separation techniques, namely conventional centrifugation and microfluidic separation, on the functionality of the isolated cells. We utilize three-dimensional refractive index distribution and time-lapse dynamics to conduct an in-depth analysis of individual WBCs, focusing on their morphology, motility, and phagocytic capabilities. Our observations highlight that centrifugal processes negatively impacts WBC motility and phagocytic capacity, whereas microfluidic separation yields a more favorable outcome in preserving WBC functionality. These findings emphasize the potential of microfluidic separation techniques as a viable alternative to traditional centrifugation for WBC isolation, potentially enabling more precise analyses in immunology research and improving the accuracy of hematologic disorder diagnoses.
Use of the Selective Cytopheretic Device to Support Critically Ill Children Requiring Continuous Renal Replacement Therapy: A Probable Benefit-Risk Assessment
Authors: Goldstein Stuart L. , Ollberding Nicholas J. , Askenazi David J. , Basu Rajit K. , Selewski David T. , Krallman Kelli A. , Yessayan Lenar , David Humes H. 
Published: August 2023

AbstractBackgroundCritically ill children with acute kidney injury (AKI) requiring continuous kidney replacement therapy (CRRT) are at increased risk of death. The selective cytopheretic device (SCD) promotes an immunomodulatory effect when circuit iCa2+is maintained at <0.40 mmol/L with regional citrate anticoagulation (RCA). In a randomized trial of adult patients on CRRT, those treated with the SCD maintaining an iCa2+<0.40 mmol/L had improved survival/dialysis independence. We have conducted two multicenter studies to evaluate safety and feasibility of the SCD in critically ill children with AKI receiving CRRT and multiorgan failure. We report the combined efficacy and safety data from these two studies for the first time.MethodsFour pediatric institutions enrolled children>10 kg in size with AKI and multiorgan dysfunction (MODS) receiving CRRT as part of standard of care to receive the SCD integrated post CRRT membrane. RCA was used to achieve a circuit iCa2+<0.40 mmol/L. We report serious adverse events, patient and CRRT-SCD related process and outcome variables and perform a Bayesian analysis to provide potential attributable benefit-risk assessment of SCD support in this critically ill population using a published matched cohort for the control population.ResultsTwenty-two patients (10 females) from the two studies comprise the combined population; 21 received mechanical ventilation, 14 received vasoactive medications, three received extracorporeal membrane oxygenation and 15 had sepsis at the time of CRRT-SCD initiation. Median SCD treatment duration was six days. Fifteen total serious adverse events were recorded, none of which were SCD related. All but one patient survived to the time of SCD discontinuation. Seventeen patients survived 60 days and 16 patients survived to the time ICU discharge. Fourteen patients surviving to ICU discharge had a normal eGFR and no patient was dialysis dependent at 60 days after CRRT-SCD initiation. Bayesian analyses revealed a 98-99% probable benefit of addition of SCD support.ConclusionThese data suggest SCD therapy is feasible, safe and demonstrates probably benefit for children who require CRRT for AKI in the setting of MODS.
Is Chinese consumers' attitude toward genetically modified foods bouncing back?
Authors: Wu Minrong , Qiao Fangbin 
Published: August 2023

PurposeThe purpose of this paper is to examine the dynamic of consumers' perceptions of GM food and explore whether their knowledge of GM technology is associated with a significant change.Design/methodology/approachThis paper presents a meta-analysis of 156 primary studies reporting a total of 225 attitudes toward genetically modified foods. To identify the impact of consumers' knowledge of GM technology, the authors estimate multivalued treatment effects model.FindingsThe results of this study show that consumers' attitudes show a U shape during 2001–2022. That is, the rise in opposition to genetically modified foods has been reversed. In addition, this study also shows that the increase in consumers' knowledge of genetically modified technology contributes to their changes in attitude.Originality/valueThis paper is the first study that empirically investigates the dynamics of Chinese consumers' attitude to genetically modified foods, and shows the rise in opposition to genetically modified foods has been reversed, which has important implication for commercialization of genetically modified crops in China.
Uncertainties affecting the offsite construction supply chain resilience: a systematic literature review
Authors: Tennakoon Tennakoon Mudiyanselage Maheshi Pabasara , Chileshe Nicholas , Rameezdeen Raufdeen , Ochoa Paniagua Jorge , Samaraweera Aparna , Statsenko Larissa 
Published: August 2023

Purpose
This paper aims to synthesise the latest literature on supply chain operations of offsite manufacturing in the construction industry to identify and evaluate the uncertainties at each stage of the offsite construction supply chain (OSC-SC) to compile contributions to knowledge and thereby determine the future research directions to improve supply chain resilience (SCR).

Design/methodology/approach
The study was conducted as a systematic literature review. In total, 99 peer-reviewed journal papers published between 2015 and mid-2022 were shortlisted and analysed through the Preferred Reporting Items for Systematic Reviews and Meta-Analyses guidelines.

Findings
The bibliometric analysis revealed that the research focusing on supply chain issues and developing best practices in offsite construction adopt case study approaches to investigate the research problems comprehensively. The content analysis revealed the lack of up-to-date knowledge in the construction workforce regarding OSC; low preparedness for the changes brought by the SC uncertainties; the lack of a proper procurement model for OSC; lack of end-to-end visibility of the supply chain to identify uncertainties and lack of national or government standards on OSC-SC, as some of the key problems instigating the identified the uncertainties.

Originality/value
This study clarifies the current knowledge by systematically presenting uncertainties at each stage of the OSC-SC. Moreover, it provides a framework and proposes that future research should focus specifically on individual/team, organisational, supply chain/network and sector/national/supranational levels to improve SCR.
Breaking down barriers of interprofessional collaboration between social work and financial and employment service organizations
Authors: Nouwen Carla , Driesen Isolde , Verharen Lisbeth , Van Regenmortel Tine 
Published: August 2023

Purpose
The growing number of people with multiple problems in different areas of life means that cross-domain interprofessional collaboration is becoming increasingly important. This study aims to focus on interprofessional collaboration between professionals from social work and financial and employment service organizations in The Netherlands. This type of cross-domain collaboration is still mostly superficial, and limited empirical knowledge is available about its beneficial factors.

Design/methodology/approach
The interprofessional collaboration model of Mulvale et al. (2016) was used as theoretical background for the research methodology and to reflect on the findings of our study. Data was collected through a qualitative study among professionals (N = 18) from social work and financial and employment service organizations in three different Dutch municipalities.

Findings
Similar team-level collaboration mechanisms of Mulvale et al.’s (2016) model were seen within this study. Joint client meetings were very beneficial for the interprofessional collaboration. Further beneficial factors include the decision-making process, team vision, client as an equal member, open communication and the appointment of a coordinator.

Research limitations/implications
Further special attention is needed to determine how best to allocate co-ordinating tasks, and how organizational and policy contexts affect the functioning of interprofessional collaborative teams.

Originality/value
This study offers an empirical view on a cross-domain collaboration between social work and financial and employment service organizations, by using Mulvale et al.’s (2016) model. In addition, this study also offers special attention to the role of the client in interprofessional collaborative teams.
An assessment of the impact of emotional labour and burnout on the employees’ work performance
Authors: Rughoobur-Seetah Soujata 
Published: August 2023

Purpose
The purpose of this study is to identify and assess the factors that influence the work performance of employees in the service sector of Mauritius during the post-COVID-19 period. Factors like emotional labour, burnout, job satisfaction and psychological health of employees have been identified as those potential factors influencing the work performance of employees.

Design/methodology/approach
This study adopts the survey-based approach for data collection. Employees from the service sector were targeted, that is, those who have direct contact with the customers. A response rate of 197 was recorded. The partial least square structural equation modelling has been used to run the data analysis.

Findings
Ten hypotheses were proposed, and four hypotheses were accepted. Job satisfaction appears to positively influence the work performance of employees in the service sector. Emotional labour, burnout and psychological health of employees appeared not to significantly affect the work performance of employees.

Practical implications
From a practical perspective, the author recommends that employers need to provide more support in terms of employee counselling, more job rotation for the employees to avoid burnout. Other support mechanisms as a supportive supervisor/manager where the employees can voice their concerns. It is also recommended that employers should have a more humane way to handle their human resources despite they are having the pressure to maximise profits. Employers need to understand that commercialisation of emotions demand a lot of emotional pressure on the employees and job re-design might be a solution to provide employees with more autonomy in the workplace. The level of flexibility also needs to be reviewed and employees should be more trusted.

Originality/value
This study acknowledges that a lot of research has been done in identifying and assessing factors that significantly impact employees’ work performance. Nevertheless, this study brings together two theories, namely, the social exchange theory and the psychological contract theory to better understand the relationship between the variables. This study also brings a methodological contribution with second-order factor analysis of factors like emotional labour and burnout which enabled better assessment and understanding of the factors and their effect on work performance. Some practical recommendations have also been made.