bluuebunny/crossref_metadata_2025_split huggingface dataset:
Hypoxia-induced Long Non-coding RNA LSAMP-AS1 Regulates ceRNA
Network to Predict Prognosis for Pancreatic Cancer
Authors: Li Lincheng , Zou Wenbo , Xiao Zhaohui , Deng Zhaoda , Liu Rong 
Published: March 2023

Background:
The limited efficacy of chemotherapy and immunotherapy for pancreatic
cancer is thought to be largely influenced by the surrounding cancer microenvironment. The hypoxic
microenvironment caused by insufficient local blood supply is very important. However, the
method to assess the level of hypoxia in the microenvironment of pancreatic cancer (PC) remains
unclear.

Methods:
In our research, we downloaded transcriptomic and clinicopathological data from the
Cancer Genome Atlas (TCGA) and Gene Expression Omnibus (GEO). A prognostic model was
developed using univariate and multivariate Cox regression. The ConsensuClusterPlus R package
was used to consistently cluster PC samples through unsupervised clustering. Gene set variation
analysis (GSVA) was performed to identify the different functional phenotypes. The CIBERSORT
evaluated the infiltration status of immune cells. qRT‐PCR was performed to detect the expression
of genes in PC cells and tissues.

Results:
A preliminary risk model was developed to reflect the hypoxic environment of pancreatic
cancer. We found that a high hypoxia risk score indicated poor long-term survival and the presence
of an immunosuppressive microenvironment. In addition, based on prognostic hypoxia-related
genes, 177 PC samples were divided into two subtypes. Compared with cluster 2, cluster 1 was defined
as the "hypoxic subgroup". The infiltration of CD8 T cells, activated memory CD4 T cells,
naive B cells, memory B cells, plasma cells, and neutrophils were lower in cluster 1, suggesting
that there was significant immunosuppression in cluster 1. Beyond that, we constructed a ceRNA
regulatory network composed of differentially expressed lncRNA, miRNA, and mRNA. LSAMPAS1/
hsa-miR-129-5p/S100A2 has been identified as a key ceRNA network that regulates the hypoxic
environment and the prognosis of PC. Notably, in our study, qRT-PCR revealed the relative
expression of LSAMP-AS1 and S100A2 was significantly upregulated in PC cells and tissue.

Conclusion:
The hypoxia-related prognostic risk model and core ceRNA network established in
our study will provide a new perspective for exploring the carcinogenic mechanism and potential
therapeutic targets of pancreatic cancer.
Understanding Recruitment Yield From Social Media Advertisements and Associated Costs of a Telehealth Randomized Controlled Trial: Descriptive Study
Authors: Aily Jéssica Bianca , Copson Jennifer , Voinier Dana , Jakiela Jason , Hinman Rana , Grosch Megan , Noonan Colleen , Armellini Megan , Schmitt Laura , White Mika , White Daniel 
Published: March 2023

Background
Recruiting study participants for clinical research is a challenging yet essential task. Social media platforms, such as Facebook, offer the opportunity to recruit participants through paid advertisements. These ad campaigns may be a cost-effective approach to reaching and recruiting participants who meet specific study criteria. However, little is known about the extent to which clicks on social media advertisements translate to the actual consent and enrollment of participants who meet the study criteria. Understanding this is especially important for clinical trials conducted remotely, such as telehealth-based studies, which open the possibility to recruit over large geographical areas and are becoming more common for the treatment of chronic health conditions, such as osteoarthritis (OA).

Objective
The aim of this study was to report on the conversion of clicks on a Facebook advertisement campaign to consent to enrollment in an ongoing telehealth physical therapy study for adults with knee OA, and the costs associated with recruitment.

Methods
This was a secondary analysis using data collected over the first 5 months of an ongoing study of adults with knee OA. The Delaware Physical Exercise and Activity for Knee Osteoarthritis program compares a virtually delivered exercise program to a control group receiving web-based resources among adults with knee OA. Advertisement campaigns were configured on Facebook to reach an audience who could be potentially eligible. Clicking on the advertisement directed potential participants to a web-based screening form to answer 6 brief questions related to the study criteria. Next, a research team member called individuals who met the criteria from the screening form and verbally asked additional questions related to the study criteria. Once considered eligible, an electronic informed consent form (ICF) was sent. We described the number of potential study participants who made it through each of these steps and then calculated the cost per participant who signed the ICF.

Results
In sum, between July and November 2021, a total of 33,319 unique users saw at least one advertisement, 9879 clicks were made, 423 web-based screening forms were completed, 132 participants were successfully contacted, 70 were considered eligible, and 32 signed the ICF. Recruitment costed an average of US $51.94 per participant.

Conclusions
While there was a low conversion from clicks to actual consent, 32% (32/100) of the total sample required for the study were expeditiously consented over 5 months with a per-subject cost well below traditional means of recruitment, which ranges from US $90 to US $1000 per participant.

Trial Registration
Clinicaltrails.gov NCT04980300; https://clinicaltrials.gov/ct2/show/NCT04980300
ABIRATERONE ACETATE LOADED SOLID LIPID NANOPARTICLES FOR IMPROVED ORAL BIOAVAILABILITY: DESIGN OF EXPERIMENTS BASED FORMULATION OPTIMIZATION, IN VITRO, EX-VIVO AND IN VIVO CHARACTERIZATION
Authors: KONATHAM SURESH , PATANGAY SHASHIKALA 
Published: March 2023

Objective: Abiraterone acetate (AA), a BCS Class IV drug, demonstrates biopharmaceutical challenges like polymorphism, poor solubility (<0.5 μg/ml), inconsistent permeability, and low oral bioavailability (<10%) (Hence requires a high dose of 1000 mg/day). The current research’s main objective is to improve oral bioavailability by manufacturing AA-loaded solid lipid nanoparticles (AA-SLNs).
Methods: SLNs were manufactured using hot homogenization followed by an ultra-sonication method. Initial screening of lipids (Glyceryl monostearate (GMS), Glyceryl Monooleate (GMO)), and surfactants (Tween 80 and Span 20) was done by mixture design. Based on statistical analysis, GMO and Tween 80 were selected for further optimization, and Central composite design (CCD) of experiments were done to optimize the composition using particle size, polydispersity index (PDI), encapsulation efficiency (EE), zeta potential, and cumulative % drug release as responses. Comparative ex-vivo and in vivo evaluations of optimized formulation were done with the pure drug and marketed formulation.
Results: Based on the statistical evaluation, GMO-4.4% and Tween 80-3.6% were optimized. Optimized AA-SLNs were found in a spherical shape with size of 286.7±12.6 nm, PDI of 0.138±0.015, EE of 94.0±1.0 %, and zeta potential of-25.0±1.0 mV. Drug release from optimized formulation was extended for 24 h, and ex-vivo permeability was increased by 2.5 and 1.42 times, whereas Relative Oral bioavailability was improved by 6.36 and 1.99 times compared to pure drug and marketed tablets, respectively.
Conclusion: The results concluded that AA-SLNs showed increased oral bioavailability compared to the pure drug and marketed formulation. Hence the dose of the formulation can be reduced to achieve the desired therapeutic effect.
EFFECTS OF THE ESSENTIAL OIL OF DRIED FRUITS OF PIPER GUINEENSE (PIPERACEAE) ON NEUROLOGICAL SYNDROMES ASSOCIATED WITH CEREBRAL MALARIA IN MICE
Authors: OPOGGEN Tivere Susan , DANIYAN Michael Oluwatoyin , ASIYANBOLA Isaac Damilare , ADEOYE Olufunso Bayo , OYEMITAN Idris Ajayi 
Published: March 2023

Background: Cerebral Malaria (CM), is associated with neurological syndromes characterized by cognitive and neurobehavioral abnormalities. Piper guineense Schum and Thonnis known to possess anti-oxidant and central nervous system activities. In this study, effects of essential oil of P. guineense (EOPG) dried fruitson selected behavioral and functional indices in mice with cerebral malaria was evaluated.
Method: Mice with confirmed CM, following intraperitoneal inoculation with 1x107 Plasmodium berghei ANKA parasitized blood in 0.2 ml of normal saline, were randomly allocated into 14 groups (n=12), namely, parasitized control, quinine control, EOPG graded doses (6.25, 12.5, 25, 50, 100 and 150mg/kg), and combination of quinine and EOPG graded doses. Quinine was administered at a dose of 20 mg/kg stat, then 10 mg/kg twice daily for next two days, while EOPG was administered once daily for 3 days, beginning from day 5 post inoculation. Non-parasitized (n=12) and parasitized controls were treated with the vehicle (5% Tween 80 in distilled water). Parasitemia, weight, survival and behavioral assessment using SHIRPA protocol were taken daily.
Results: EOPG showed anti-plasmodial activity in a dose dependent manner, significantly mitigated mortality rate at higher doses (100 and 150mg/kg), and exhibit dose dependent central nervous system protective effects. Also, except for quinine/6.25 mg/kg EOPG, 100% mortality was observed with combination groups, suggesting a potential to precipitate toxicity.
Conclusion: The study concluded that EOPG possesses antimalarial and central nervous system protective effects and may therefore serves to mitigate neurological syndrome in cerebral malaria.

Peer Review History:
Received: 20 December 2022; Revised: 27 January 2023; Accepted: 28 February, Available online: 15 March 2023
Academic Editor: Dr. Jennifer Audu-Peter, University of Jos, Nigeria, drambia44@gmail.com
Received file: Reviewer's Comments:
Average Peer review marks at initial stage: 6.5/10
Average Peer review marks at publication stage: 7.5/10
Reviewers:
Dr. Rima Benatoui,Laboratory of Applied Neuroendocrinology, Department of Biology, Faculty of Science, Badji Mokhtar University Annaba, Algeria. benattouiryma@gmail.com
Dr. Nuray Arı, Ankara University, Turkiye, ari@ankara.edu.tr
Analisis Strategi Pemasaran Pada Era New Normal di Mano Beach House Restaurant Seminyak
Authors: Widiantari Ni Kadek , Amir Firlie Lanovia , Praminatih Gusti Ayu 
Published: March 2023

Penelitian ini membahas strategi pemasaran di Mano Beach House Restaurant dengan tujuan untuk menganalis faktor-faktor internal dan eksternal, untuk mengetahui posisi strategi pemasaran yang telah dilakukan, serta untuk mengetahui strategi pemasaran alternatif yang dapat diterapkan di Mano Beach House Restaurant. Teknik pengumpulan data dalam penelitian ini adalah dengan melakukan wawancara, observasi, dokumentasi, dan juga penyebaran kuesioner kepada 4 responden. Teknik analisis data yang digunakan yaitu deskriptif kualitatif, IFAS-EFAS dan analisis SWOT. Hasil penelitian dengan analisis SWOT didapat titik koordinat sumbu X (internal) = 2,65 dan sumbu Y (eksternal) = 1,32 sehingga posisi Mano Beach House berada pada kuadran I, yakni mendukung strategi agresif (growth oriented strategy). Strategi alternatif yang dapat diterapkan oleh Mano Beach House Restaurant adalah dengan memanfaatkan teknologi digital seperti media sosial yang dimiliki dalam meningkatkan kegiatan promosi produk-produk yang dimiliki Mano Beach House Restaurant dengan membuat konten yang menarik dan mengarah kepada positioning perusahaan, serta menjaga dan meningkatkan kerja sama dengan mitra kerja baik travel agent maupun ojek online dalam mempromosikan produk. Ojek online sangat mendukung pendistribusian produk secara online di era New Normal saat ini.

This study discusses the marketing strategy at Mano Beach House Restaurant with the aim of analyzing internal and external factors, to determine the position of the marketing strategy that has been carried out, and to find out alternative marketing strategies that can be applied at Mano Beach House Restaurant. Data collection techniques in this study were by conducting interviews, observation, documentation, and also distributing questionnaires to 4 respondents. Data analysis techniques used are descriptive qualitative, IFAS-EFAS and SWOT analysis. The results of the research using SWOT analysis obtained the coordinates of the X axis (internal) = 2.65 and the Y axis (external) = 1.32 so that the position of Mano Beach House is in quadrant I, which supports an aggressive strategy (growth oriented strategy). An alternative strategy that can be applied by Mano Beach House Restaurant is to utilize digital technology such as social media that is owned in increasing promotional activities for the products owned by Mano Beach House Restaurant by creating interesting content that leads to the company's positioning, as well as maintaining and enhancing cooperation. with business partners both travel agents and online motorcycle taxis in promoting products. Online motorcycle taxis strongly support the distribution of products online in the current New Normal era.
Abatacept for Delay of Type 1 Diabetes Progression in Stage 1 Relatives at Risk: A Randomized, Double-Masked, Controlled Trial
Authors: Russell William E. , Bundy Brian N. , Anderson Mark S. , Cooney Laura A. , Gitelman Stephen E. , Goland Robin S. , Gottlieb Peter A. , Greenbaum Carla J. , Haller Michael J. , Krischer Jeffrey P. , Libman Ingrid M. , Linsley Peter S. , Long S. Alice , Lord Sandra M. , Moore Daniel J. , Moore Wayne V. , Moran Antoinette M. , Muir Andrew B. , Raskin Philip , Skyler Jay S. , Wentworth John M. , Wherrett Diane K. , Wilson Darrell M. , Ziegler Anette-Gabriele , Herold Kevan C. ,   Type 1 Diabetes TrialNet Study Group
Published: March 2023

OBJECTIVEPrevious studies showed that inhibiting lymphocyte costimulation reduces declining β-cell function in individuals newly diagnosed with type 1 diabetes. We tested whether abatacept would delay or prevent progression of type 1 diabetes from normal glucose tolerance (NGT) to abnormal glucose tolerance (AGT) or to diabetes and the effects of treatment on immune and metabolic responses.RESEARCH DESIGN AND METHODSWe conducted a phase 2, randomized, placebo-controlled, double-masked trial of abatacept in antibody-positive participants with NGT who received monthly abatacept/placebo infusions for 12 months. The end point was AGT or diabetes, assessed by oral glucose tolerance tests.RESULTSA total of 101 participants received abatacept and 111 placebo. Of these, 81 (35 abatacept and 46 placebo) met the end point of AGT or type 1 diabetes diagnosis (hazard ratio 0.702; 95% CI 0.452, 1.09; P = 0.11) The C-peptide responses to oral glucose tolerance tests were higher in the abatacept arm (P &lt; 0.03). Abatacept reduced the frequency of inducible T-cell costimulatory (ICOS)+ PD1+ T-follicular helper (Tfh) cells during treatment (P &lt; 0.0001), increased naive CD4+ T cells, and also reduced the frequency of CD4+ regulatory T cells (Tregs) from the baseline (P = 0.0067). Twelve months after treatment, the frequency of ICOS+ Tfh, naive CD4+ T cells, and Tregs returned to baseline.CONCLUSIONSAlthough abatacept treatment for 1 year did not significantly delay progression to glucose intolerance in at-risk individuals, it impacted immune cell subsets and preserved insulin secretion, suggesting that costimulation blockade may modify progression of type 1 diabetes.
Nannizziopsis guarroi has prolonged environmental persistence on clinically relevant substrates
Authors: Keller Krista A. , Durante Kennymac , Foltin Eliana , Cerreta Anthony J. 
Published: March 2023

Abstract

OBJECTIVE
To determine the environmental persistence of Nannizziopsis guarroi on clinically relevant solid and aqueous substrates.

SAMPLE
2 molecularly confirmed isolates of N guarroi obtained from clinical cases of dermatomycosis in bearded dragons (Pogona vitticeps).

PROCEDURES
3 concentrations (1 McFarland, 1:10 McFarland, and 1:100 McFarland) of fungal suspension were exposed to 7 sterilized solid substrates (fabric aquarium liner, wood mulch, sand, hard plastic, glass, cotton, and stainless steel) and 2 sterilized aqueous substrates (distilled water, saline solution [0.9% NaCl]). Biological replicates were performed for the contamination of the solid substrates. On days 1, 3, and 14 after contamination, the substrates were sampled for fungal culture with technical repeat. Fungal cultures were incubated at room temperature for 10 days and then evaluated for fungal growth.

RESULTS
Data from wood mulch were not evaluated because of plate contamination. Overall, the ability to culture N guarroi from solid substrates was isolate, time, and fungal concentration dependent. Viable fungus was isolated from fabric aquarium liner and glass on day 1 and days 1 and 3, respectively. N guarroi was cultured from all other solid substrates at day 14 from at least 1 isolate and/or fungal concentration. Viable N guarroi was isolated from both aqueous substrates at day 14, regardless of isolate or fungal concentration.

CLINICAL RELEVANCE
The environmental persistence of N guarroi should be considered when treating lizards infected with this fungus. Fomites may contribute to the contagious nature of this pathogen and environmental disinfection should be performed to reduce transmission.
Drivers of Regional Economic Growth in Hungary before and during the Pandemic Crisis
Authors: Zsibók Zsuzsanna 
Published: March 2023

Abstract
The 2010s saw a gradual improvement in economic growth in Hungary, which was noticeable in all regions. In this research, we examine the post-crisis economic development trends in a NUTS 3 level disaggregation in the context of the dependent market economy model. The research uses descriptive and multivariate statistical analysis to shed light on the main regional trends. Notable changes occurred mainly in the top and middle performing counties in terms of output, while the bottom of the ranking was characterized by stagnation. Territorial disparities have temporarily narrowed, but there has been no significant catching up of lagging regions.
Fall Risk in Adult Family Practice Non-Attenders: A Cross-Sectional Study from Slovenia
Authors: RUŽIĆ-GORENJEC Nina , KLEMENC KETIŠ Zalika , BLAGUS Rok , POPLAS SUSIČ Antonija 
Published: March 2023

ABSTRACTIntroductionNot much is known about the fall risk among the adult population of those who rarely visit doctors. We wanted to determine the prevalence of increased fall risk in a population of family practice non-attenders and the factors associated with it.MethodsWe included participants from family medicine practices in this cross-sectional study. To be included in the study, the participants had to be adults living in the community (home-dwelling people) who had not visited their chosen family physician in the last five years (non-attenders). The identification of the eligible persons was done through a search of electronic medical records, which yield 2,025 non-attenders. Community nurses collected data in the participants’ homes. The outcome measure was increased fall risk as assessed by the Morse fall scale: increased risk (≥25) vs. no risk.ResultsThe sample consisted of 1,945 patients (96.0% response rate) with a mean age of 60.4 years (range 20.5 to 99.7 years). An increased fall risk was determined in 482 or 24.8% (95% CI: [22.9, 26.8]) of the patients. The multivariate model showed a significant association of increased fall risk with higher age (p<0.001), lower systolic blood pressure (p=0.047), poor family function (p=0.016), increased risk of malnutrition (p=0.013), higher number of chronic diseases (p=0.027), higher pain intensity (p<0.001), lower self-assessment of current health (p=0.002), and higher dependence in daily activities (p<0.001).ConclusionNon-attenders may have an increased risk of falling which depends on their health status and age. The inclusion of community nurses in primary healthcare teams could be of use not only to identify the non-attenders’ health needs, but also to better manage their health, especially the factors that were identified to be associated with greater fall risk.
Psychological Well-Being and Resilience of Slovenian Students During the COVID-19 Pandemic
Authors: ROPRET Nina , KOŠIR Urška , ROŠKAR Saška , KLOPČIČ Vito , VRDELJA Mitja 
Published: March 2023

ABSTRACT

Introduction
Students’ mental health is recognised as an important public health issue, and the strict measures and many changes resulting from the COVID-19 pandemic may have exacerbated this. The aims of the study were thus to explore psychological well-being among university students in Slovenia during the beginning of the second lockdown, and to assess associations among their psychological well-being, demographic characteristics, presence of a chronic health condition, and resilience.

Methods
The Slovenian online cross-sectional survey was performed as part of a large-scale international survey led by the COVID-HL Consortium, between the 2nd and 23rd November 2020. The study was carried out on a sample of 3,468 university students (70% female) in Slovenia, aged between 18 to 40 (M=22/SD=3). In addition to sociodemographic data and that on the presence of a chronic health condition, data on subjective social status (SSS), psychological well-being (WHO-5) and resilience (CD-RISC 10) was also gathered.

Results
In our study 52% of university students reported good psychological well-being. Hierarchical binary logistic regression revealed that male, older students, those with higher perceived subjective social status, students without a chronic health condition, and those with higher score on resilience were more likely to have good psychological well-being. Resilience was the strongest predictor of psychological well-being in our study.

Conclusions
Systematic preventive approaches/interventions in the field of mental health should be implemented among students in Slovenia. In this context it is important to develop and deliver programmes for enhancing resilience, which is an important protective factor in times of mental distress.
PELUANG USAHA: Ide Usaha Yang Kreatif BUSINESS OPPORTUNITIES: Creative Business Ideas
Authors: Masnita Yolanda , Khomsiyah Khomsiyah , Nurhaida Dida 
Published: March 2023

Meningkatkan nilai tambah produk, saat ini menjadi syarat penting untuk dapat menghadapi persaingan pasar yang semakin ketat. Salah satunya dengan mengolah bahan baku menjadi barang setengah jadi atau produk siap jual. Pondok Pesantren (Ponpes) Madinah Al Hijrah, yang terletak di Kampung Halimun, Desa Cimanuk, Kabupaten Pandegang, Provinsi Banten memiliki potensi alam dan lingkungan yang mendukung untuk pengembangan budidaya pertanian, perikanan dan produk olahan makanan. Awalnya Ponpes sebagai mitra PkM hanya membudidayakan lele dan menjual nya secara langsung. Berdasarkan identifikasi masalah dirumuskan bahwa dapat meningkatkan nilai jual produk dengan mengolahnya menjadi beberapa produk makanan olahan. Kegiatan PkM ini ditujukan untuk mendukung ide mengembangkan pendapatan ponpes dan meningkatkan keahlian santri, yang difokuskan pada upaya mempertahankan kelangsungan usaha serta meningkatkan kesejahteraan santri melalui penciptaan dan penambahan nilai produk melalui pendekatan ekonomi syariah, manajemen pemasaran, dan akuntansi.. Metode pelaksanaan berupa penyuluhan dan simulasi guna memberikan gambaran mengenai proses penciptaan kreasi dan diversifikasi produk makanan olahan yang lebih variative. Peserta kegiatan sangat antusias, aktif dalam diskusi dan telah memiliki pemahaman serta mampu mengubah pola pikir tradisionalnya menjadi lebih kreatif. Hal ini dibuktikan dengan rencana tindakan berupa diversifikasi produk lele mentah menjadi aneka rupa lele olahan.