bluuebunny/crossref_metadata_2025_split huggingface dataset:
Ficolin‐3 and MASP‐2 gene variants in Siberian arctic populations: Summarized evidence of selective pressure for the high frequency of lectin complement pathway deficiency
Authors: Tereshchenko Sergey Yu. , Smolnikova Marina V. , Freidin Maxim B. 
Published: December 2022

AbstractHerewith, we provide novel original data about the prevalence of FCN3 rs532781899 and MASP2 rs72550870 variants among the newborns of aboriginal Siberian Arctic populations (Nenets and Dolgan‐Nganasans) and Russians of East Siberia. This novel data has been analysed along with the genetic data about other proteins of the lectin pathway of the complement system (mannose‐binding lectin and ficolin‐2) obtained earlier. A total of 926 specimens of dried blood spots of the newborns were genotyped. The newborns represented four populations: Nenets, Dolgan‐Nganasans, Mixed aboriginal population, and Russians (Caucasians) to study the prevalence of single nucleotide polymorphisms of FCN3 rs532781899 and MASP2 rs72550870. The prevalence of the deletion allele of the rs532781899 variant in the FCN3 gene associated with the decreased production of ficolin‐3 was found to be increased in Russians compared to the Nenets aboriginal populations (P = .002). The prevalence of the rs72550870*G allele in the MASP2 gene associated with low serum protease activity was found to be increased in Russians compared with Nenets and Dolgan‐Nganasans (P < .001 and P = .03, respectively). The results of the current study and our previous findings corroborate with a hypothesis that human evolution has been directed toward the accumulation of genotypes associated with low activity of the lectin complement activation pathway.
The plant peptide hormone phytosulfokine promotes somatic embryogenesis by maintaining redox homeostasis in Cunninghamia lanceolata
Authors: Hao Zhaodong , Wu Hua , Zheng Renhua , Li Rui , Zhu Zeli , Chen Ya , Lu Ye , Cheng Tielong , Shi Jisen , Chen Jinhui 
Published: December 2022

SUMMARYSomatic embryogenesis (SE) is widely used for studying the mechanisms of embryo development. However, little is known about the underlying mechanisms, especially in woody plants. Previous studies have established an SE system for Chinese fir (Cunninghamia lanceolata), but this system is genotype‐dependent, which limits its application in practice. Here, we found that phytosulfokine (PSK), a plant peptide hormone, can not only increase SE efficiency, but also establish SE in recalcitrant genotypes of C. lanceolata. Proembryogenic mass (PEM) browning and determination of hydrogen peroxide (H2O2) content by 2′,7′‐dichlorofluorescein staining indicated that a reactive oxygen species (ROS) burst occurred rapidly after PEMs were transferred to SE induction medium. Transcriptome analysis and quantitative reverse transcriptase‐PCR validation showed that PSK treatment helped to maintain ROS homeostasis by decreasing the activity of peroxidases in early SE induction. This PSK‐regulated redox microenvironment might be helpful to induce expression of SE‐related genes like WOX2 in early SE induction. Further analyses suggested that PSK promotes SE induction in C. lanceolata partially through decreasing H2O2 levels, which is necessary but not sufficient for SE induction in recalcitrant genotypes of C. lanceolata. Furthermore, heterologous overexpression of ClPSK in Arabidopsis led to enhanced SE induction and resistance to H2O2 stress. Taken together, our study reveals a biological function for the plant peptide hormone PSK, extends our knowledge about SE in woody trees, and provides a valuable tool for establishing an efficient and genotype‐independent SE system in C. lanceolata and other coniferous trees.
Direct access to millions of mutations by whole genome sequencing of an oilseed rape mutant population
Authors: Jhingan Srijan , Kumar Avneesh , Harloff Hans‐Joachim , Dreyer Felix , Abbadi Amine , Beckmann Katrin , Obermeier Christian , Jung Christian 
Published: December 2022

SUMMARYInduced mutations are an essential source of genetic variation in plant breeding. Ethyl methanesulfonate (EMS) mutagenesis has been frequently applied, and mutants have been detected by phenotypic or genotypic screening of large populations. In the present study, a rapeseed M2 population was derived from M1 parent cultivar ‘Express’ treated with EMS. Whole genomes were sequenced from fourfold (4×) pools of 1988 M2 plants representing 497 M2 families. Detected mutations were not evenly distributed and displayed distinct patterns across the 19 chromosomes with lower mutation rates towards the ends. Mutation frequencies ranged from 32/Mb to 48/Mb. On average, 284 442 single nucleotide polymorphisms (SNPs) per M2 DNA pool were found resulting from EMS mutagenesis. 55% of the SNPs were C → T and G → A transitions, characteristic for EMS induced (‘canonical’) mutations, whereas the remaining SNPs were ‘non‐canonical’ transitions (15%) or transversions (30%). Additionally, we detected 88 725 high confidence insertions and deletions per pool. On average, each M2 plant carried 39 120 canonical mutations, corresponding to a frequency of one mutation per 23.6 kb. Approximately 82% of such mutations were located either 5 kb upstream or downstream (56%) of gene coding regions or within intergenic regions (26%). The remaining 18% were located within regions coding for genes. All mutations detected by whole genome sequencing could be verified by comparison with known mutations. Furthermore, all sequences are accessible via the online tool ‘EMSBrassica’ (http://www.emsbrassica.plantbreeding.uni‐kiel.de), which enables direct identification of mutations in any target sequence. The sequence resource described here will further add value for functional gene studies in rapeseed breeding.
The Role of Athlete Leadership Quality in the Characteristics of Team Resilience in Elite Soccer Teams: A Cross-Sectional and Longitudinal Mediation of Team Identification
Authors: López-Gajardo Miguel A. , González-Ponce Inmaculada , García-Calvo Tomás , Enrich-Alturo Edgar , Leo Francisco M. 
Published: December 2022

We present two studies examining the relationship between athlete leadership quality and team resilience and explored the mediating effect of team identification. In Study 1, 194 soccer players (Mage = 18.50, SD = 4.49) from eight national teams participated. Structural equation modeling showed cross-sectionally that the four types of athlete leadership qualities were positively related to the characteristics of resilience and negatively to vulnerability under pressure. Team identification was shown to be a mediator of these relationships. Study 2, with four different time-points, involved 208 young soccer players (Mage = 16.05, SD = 3.39) from two professional clubs (i.e., La Liga). Cross-lagged panel models revealed that task leadership quality (Times 1–2) was positively related to the characteristics of resilience (Times 3–4) and negatively to vulnerability under pressure (Times 3–4). However, team identification did not mediate these relationships. Therefore, practitioners should consider the perceptions of leader quality to achieve benefits during competition.
Hydraulic Habitat Structure Impacts Risk of Trematode Infection
Authors: Mikheev V. N. , Pasternak A. F. , Taskinen J. 
Published: December 2022

Abstract—Variability in infection rates of trematodesDiplostomum pseudospathaceumin 0+ rainbow troutOncorhynchus mykissunder different hydraulic regimes was studied in the experimental setup with regulated flow rates and environmental heterogeneity. The average infection rate in the conditions of the current was 1.5 times less than that in the stagnant water. An increase of the flow rate from 3.2 to 11.3 cm/s did not result in lower infection rates, while the interindividual variability in the infection rate (coefficient of variation) tended to increase along with changing the still-water conditions (20%) to the high flow rate environments (40%) within the experiment. A decrease in the average infection rate and an increase in the variability within the heterogenous flow of water could indicate the fact that some fish effectively avoid infection. It may be caused by redistribution of cercariae suspended in the water column across microhabitats with different flow rates together with fish behavior which allows them to choose sites with low parasite concentration.
Thermodynamics of asymptotically de Sitter black hole in dRGT massive gravity from Rényi entropy
Authors: Chunaksorn Phuwadon , Hirunsirisawat Ekapong , Nakarachinda Ratchaphat , Tannukij Lunchakorn , Wongjun Pitayuth 
Published: December 2022

AbstractThe thermodynamic properties of the de Rham–Gabadadze–Tolley (dRGT) black hole in the asymptotically de Sitter (dS) spacetime are investigated by using Rényi entropy. It has been found that the black hole with asymptotically dS spacetime described by the standard Gibbs–Boltzmann statistics cannot be thermodynamically stable. Moreover, there generically exist two horizons corresponding to two thermodynamic systems with different temperatures, leading to a nonequilibrium state. Therefore, in order to obtain the stable dRGT black hole, we use the alternative Rényi statistics to analyze the thermodynamic properties in both the separated system approach and the effective system approach. Interestingly, we found that it is possible concurrently obtain positive pressure and volume for the dRGT black hole while it is not for the Schwarzschild-de Sitter (Sch-dS) black hole. Furthermore, the bounds on the nonextensive parameter for which the black hole being thermodynamically stable are determined. In addition, the key differences between the systems described by different approaches, e.g., temperature profiles and types of the Hawking–Page phase transition are pointed out.
A Genomic Analysis of Bacillus megaterium HT517 Reveals the Genetic Basis of Its Abilities to Promote Growth and Control Disease in Greenhouse Tomato
Authors: Yang Wei , Zhao Yingnan , Yang Yang , Zhang Minshuo , Mao Xiaoxi , Guo Yanjie , Li Xiangyu , Tao Bu , Qi Yongzhi , Ma Li , Liu Wenju , Li Bowen , Di Hong J. 
Published: December 2022

Bacillus megaterium is well known as a plant growth-promoting rhizobacterium, but the relevant molecular mechanisms remain unclear. This study aimed to elucidate the effects of B. megaterium HT517 on the growth and development of and the control of disease in greenhouse tomato and its mechanism of action. A pot experiment was conducted to determine the effect of B. megaterium on tomato growth, and this experiment included the HT517 group (

3.2
×

10

8

cfu/pot) and the control group (inoculated with the same amount of sterilized suspension). An antagonistic experiment and a plate confrontation experiment were conducted to study the antagonistic effect of B. megaterium and Fusarium oxysporum f.sp. lycopersici. Liquid chromatography–mass spectrometry was used to determine the metabolite composition and metabolic pathway of HT517. PacBio+Illumina HiSeq sequencing was utilized for map sequencing of the samples. An in-depth analysis of the functional genes related to the secretion of these substances by functional bacteria was conducted. HT517 could secrete organic acids that solubilize phosphorus, promote root growth, secrete auxin, which that promotes early flowering and fruiting, and alkaloids, which control disease, and reduce the incidence of crown rot by 51.0%. The complete genome sequence indicated that the strain comprised one circular chromosome with a length of 5,510,339 bp (including four plasmids in the genome), and the GC content accounted for 37.95%. Seven genes (pyk, aceB, pyc, ackA, gltA, buk, and aroK) related to phosphate solubilization, five genes (trpA, trpB, trpS, TDO2, and idi) related to growth promotion, eight genes (hpaB, pheS, pheT, ileS, pepA, iucD, paaG, and kamA) related to disease control, and one gene cluster of synthetic surfactin were identified in this research. The identification of molecular biological mechanisms for extracellular secretion by the HT517 strain clarified that its organic acids solubilized phosphorus, that auxin promoted growth, and that alkaloids controlled tomato diseases.
Correlations between Visual Performance and Chorioretinal Variables after Vitrectomy for the Idiopathic Macular Hole
Authors: Chen Minfeng , Zheng Chenchen , Liu Yu , Chen Feng , Xu Dan , Lu Fan 
Published: December 2022

Purpose. To investigate the relationships between visual function and the retinal and choroid microstructure in idiopathic macular hole patients after surgery. Methods. A prospective study investigated changes in the fundus structure and visual function in 16 macular hole patients before and after surgery. Patients’ best-corrected visual acuity (BCVA) and retinal sensitivity were measured by an EDTRS visual chart and microperimetry (MP1), respectively. The thickness of the retina and the blood supply to the retina and superficial choroid were detected by OCTA, and the choroidal capillary blood flow density was analysed with MATLAB. The thickness of the choroid and the aperture size of the macular hole were detected by Heidelberg OCT. Results. Compared with before surgery and one month after surgery, the BCVA (3 months: 0.47 ± 0.27, before: 1.02 ± 0.22, 1 month: 0.66 ± 0.27, and

P

< 0.05) and the central sensitivity of the retina (3 months: 14.88 ± 2.87 dB, before: 8.76 ± 3.27 dB, 1 month: 12.22 ± 3.30 dB, and

P

< 0.05) were significantly improved three months after surgery. The change in BCVA was significantly correlated with the basal diameter (r = 0.677 and

P

= 0.004), the minimum diameter (r = 0.585 and

P

= 0.017), the macular hole cystoid height area index (r = −0.618 and

P

= 0.011), the central macular hole index (r = −0.727 and

P

= 0.001), the peripheral macular hole index (r = −0.758 and

P

= 0.001), the central tractional hole index (r = −0.717 and

P

= 0.002), the peripheral tractional hole index (r = −0.725 and

P

= 0.001), and changes in the peripheral blood vessel density of the choroid capillary layer (r = 0.585 and

P

= 0.0017). The change in central retinal sensitivity was correlated with the change in the superficial foveal avascular zone (FAZ; r = 0.520 and

P

= 0.039), change in the retinal superficial peripheral blood flow density (r = −0.503 and

P

= 0.047), change in the deep FAZ (r = 0.599 and

P

= 0.014), and change in the retinal deep peripheral blood flow density (r = −0.601 and

P

= 0.014). Conclusions. The morphology of the macular hole as well as changes to the retinal and choroidal microstructure contributes to the recovery of visual function after surgery.
CD137 (4-1BB)-Based Cancer Immunotherapy on Its 25th Anniversary
Authors: Melero Ignacio , Sanmamed Miguel F. , Glez-Vaz Javier , Luri-Rey Carlos , Wang Jun , Chen Lieping 
Published: December 2022

AbstractTwenty-five years ago, we reported that agonist anti-CD137 monoclonal antibodies eradicated transplanted mouse tumors because of enhanced CD8+ T-cell antitumor immunity. Mouse models indicated that anti-CD137 agonist antibodies synergized with various other therapies. In the clinic, the agonist antibody urelumab showed evidence for single-agent activity against melanoma and non-Hodgkin lymphoma but caused severe liver inflammation in a fraction of the patients. CD137's signaling domain is included in approved chimeric antigen receptors conferring persistence and efficacy. A new wave of CD137 agonists targeting tumors, mainly based on bispecific constructs, are in early-phase trials and are showing promising safety and clinical activity.Significance:CD137 (4-1BB) is a costimulatory receptor of T and natural killer lymphocytes whose activity can be exploited in cancer immunotherapy strategies as discovered 25 years ago. Following initial attempts that met unacceptable toxicity, new waves of constructs acting agonistically on CD137 are being developed in patients, offering signs of clinical and pharmacodynamic activity with tolerable safety profiles.
Old Icelandic veri
Authors: de Leeuw van Weenen Andrea 
Published: December 2022

Abstract
In Modern Icelandic the form veri of the verb vera ‘to be’ is seen as a subjunctive expressing a wish. Treating Old Norse veri, earlier vesi, as an imperative of the third person simplifies the vera paradigm. A survey of the oldest attestations shows that veri not only fits qua form in the imperative paradigm, but also behaves like an imperative and expresses a command. The hypothesis that veri is an imperative can be extended to: Old Norse had an imperative of the 3rd person consisting of stem+i. What usually is called the use of the 3rd person subjunctive to fill in for the missing 3rd person imperative, would then be nothing else than a real imperative, which, however, in all verbs except vera coincides in form with the subjunctive. The form verir looks like a counter example to the hypothesis, but it is only found twice in poetry, never in prose, and can be explained as a common copying error. We cannot ask the native speakers of Old Norse, so the description of Old Norse veri as a subjunctive is a hypothesis as well. It is argued that seeing veri as an imperative is the more elegant solution.
“Let the Sirens Roar”: The Women’s Protests in Poland and the Artistic Response to the Backlash
Authors: Stępnik Małgorzata 
Published: December 2022

“Let the Sirens Roar”: The Women’s Protests in Poland and the Artistic Response to the BacklashThe ruling of the Constitutional Tribunal of 22 October 2020, which tightened the restrictions on reproductive rights in Poland and was adopted in the midst of the COVID-19 pandemic, sparked strong social opposition and massive demonstrations. The article focuses on artistic works accompanying these protests, providing a kind of visual commentary to them or, in other words, their visual “framework”. The discussion refers to the most characteristic iconographic materials, mainly posters, uploaded and shared by (mostly young) artists on their profiles on popular social media and specially created websites (such as Graphic Emergency). The content of this artistic “production” often refers to important moments in the recent history of Poland (the Solidarity movement), traditional symbols, and the imaginational sphere; however, to some extent, it also refers to the countercultural revolution of the late 1960s, especially to L’Atelier Populaire, established by students of Académie des Beaux Arts in Paris. The article proposes that the discussed posters be interpreted as a generational counterreply to the “backlash”, understood in accordance with the meaning given to that word by Susan Faludi in her classic 1991 study. „Niech wyją syreny”. Kobiece protesty w Polsce i artystyczna odpowiedź na „backlash”Orzeczenie Trybunału Konstytucyjnego z 22 października 2020 roku, ograniczające prawa reprodukcyjne w Polsce – przyjęte w środku pandemii COVID-19 – wywołało silny sprzeciw społeczny i masowe demonstracje. Tematyka artykułu zogniskowana jest wokół prac artystycznych towarzyszących owym protestom, stanowiących ich wizualny komentarz, innymi słowy: ich wizualną „oprawę”. W niniejszych rozważaniach odniesiono się do najbardziej charakterystycznych materiałów ikonograficznych, głównie plakatów, zamieszczanych i udostępnianych przez (przeważnie młodych) artystów na profilach w popularnych mediach społecznościowych oraz na specjalnie wykreowanych witrynach (jak np. Pogotowie Graficzne). Zawartość treściowa tej twórczej „produkcji” często odnosi się do ważnych momentów w najnowszej historii Polski (Solidarność), tradycyjnej symboliki i sfery wyobrażeniowości, a w pewnym względzie także i do kontrkulturowej rewolty późnych lat 60., zwłaszcza do L’Atelier Populaire założonego przez studentów paryskiej Académie des Beaux Arts. W artykule wskazano, iż omawiane plakaty można interpretować jako pokoleniową odpowiedź na „backlash” w takim rozumieniu, jakie nadała temu słowu Susan Faludi w swej klasycznej już rozprawie z 1991 roku.
“The Lexicon of Migrating Ideas in the Slavic Balkans” and the Risk of Knowledge: Some Considerations Regarding Two Promotional Events for This Book
Authors: Drzewiecka Ewelina 
Published: December 2022

The Lexicon of Migrating Ideas in the Slavic Balkans and the Risk of Knowledge: Some Considerations Regarding Two Promotional Events for This Book
The text is an account of two events that promoted the extensive (ten-volume) monograph by Polish Slavic studies scholars (with contributions from scholars from a number of foreign research centres): Leksykon idei wędrownych na słowiańskich Bałkanach (XVIII – XXI w.) [The Lexicon of Migrating Ideas in the Slavic Balkans (18th-21st centuries)] (2018–2020). These events were held in 2021, during the COVID-19 pandemic, by means of social media and common communication platforms. As the Lexicon consists of many synthetically expressed, original, and source-based insights on the southern Slavic cultures’ struggles with modernity, the author comments on both the research project at the root of this publication and on the course of the discussion which emerged during these meetings. She does so in order to express her view on the capacity of the modern researcher by adapting Olga Tokarczuk’s notion of the tender narrator.

Leksykon idei wędrownych na słowiańskich Bałkanach a ryzyko wiedzy. Rozmyślania wokół dwóch wydarzeń promujących publikację
Artykuł stanowi sprawozdanie z dwóch wydarzeń promujących dziesięciotomową monografię autorstwa kolektywu polskich slawistów (we współpracy z badaczami z kilku zagranicznych ośrodków naukowych) – Leksykon idei wędrownych na słowiańskich Bałkanach (XVIII – XXI w.) (2018–2020), jakie zostały zorganizowane za pośrednictwem platform społecznościowych oraz komunikacyjnych w 2021 roku, a więc w warunkach pandemii COVID-19. Autorka najpierw prezentuje sam projekt naukowy, który legł u podstaw publikacji, wskazując, że Leksykon zawiera wiele syntetycznie wyrażonych, oryginalnych, opartych na badaniach źródłowych refleksji na temat spotkania z nowoczesnością na gruncie kultur południowosłowiańskich. Następnie referuje przebieg dyskusji, jakie wynikły w trakcie obu spotkań promocyjnych, po czym odnosi się do kwestii kompetencji nowoczesnego naukowca, adaptując pojęcia czułego narratora Olgi Tokarczuk.
Lessons from the Past: The Right to Abortion in Three Pictures
Authors: Milinković Jelena 
Published: December 2022

Lessons from the Past: The Right to Abortion in Three PicturesThe starting point in this paper is the current situation in several countries regarding the prohibition or permission of abortion. In parallel with the pandemic, i.e., the COVID-19 crisis in European countries, there is also a crisis of women’s rights and freedoms. Within the already won rights and freedoms, and as a consequence of the traditionalization of state policies, the right to abortion is most endangered during crises. To show what the struggle for women’s rights might look like, an example from the past is taken: the struggle of Yugoslav women for the right to abortion during the 1920s and 1930s. Three aspects are analyzed: 1) the legal regulation of the right to abortion, 2) the discussion regarding this issue in the Ženski pokret [Women’s Movement] (1920–1938) journal, and 3) the topic of abortion in literature.Lekcja z przeszłości. Prawo do aborcji w trzech obrazachPunktem wyjścia w artykule jest aktualna sytuacja w kilku krajach dotycząca zakazu lub zezwolenia na aborcję. Równolegle z pandemią, czyli kryzysem COVID-19 w krajach europejskich, następuje również kryzys praw i wolności kobiet. W ramach wywalczonych już praw i wolności oraz w wyniku tradycjonalizacji polityki państwa prawo do aborcji jest najbardziej zagrożone w czasie kryzysów. Aby pokazać, jak mogłaby wyglądać walka o prawa kobiet, posłużono się przykładem z przeszłości dotyczącym walki kobiet jugosłowiańskich o prawo do aborcji w latach 20. i 30. XX wieku. Analizie poddane zostały trzy kwestie: 1) prawne uregulowanie prawa do aborcji, 2) dyskusja na ten temat w czasopiśmie „Ženski pokret” [„Ruch kobiet”] (1920–1938), 3) temat aborcji w literaturze.