bluuebunny/crossref_metadata_2025_split huggingface dataset:
Extremely High Concentrations of Ice Particles in East Asian Dust‐Infused Baroclinic Storm (DIBS) Cirrus Shield: Dominant Role of Dust Ice Nucleation Effect
Authors: Zeng Yi , Wang Minghuai , Zhao Chun , Zhu Yannian , Rosenfeld Daniel , Huang Kang‐En 
Published: March 2023

AbstractActing as efficient ice‐nucleating particles, dust particles can affect cirrus cloud properties through heterogeneous nucleation. A dust‐infused baroclinic storm (DIBS) over East Asia in May 2017 was examined using satellite observations and the Weather Research and Forecasting model coupled with chemistry (WRF‐Chem) to study the dust effects on cirrus clouds. Satellite observations showed that the ice number concentration (Nice) was extremely high, exceeding 1–10 cm−3 at the cirrus shield of the DIBS, and the ice effective radius (rei) was about 10–30 μm. An aerosol‐aware heterogeneous ice nucleation parameterization (Ullrich et al., 2017, https://doi.org/10.1175/jas-d-16-0074.1) was implemented into WRF‐Chem to explore the formation mechanism of such a high Nice. The simulated Nice from Ullrich17 was 1–2 orders of magnitude higher than that from the default scheme (Cooper, 1986, https://doi.org/10.1007/978-1-935704-17-1_4), and the rei was 2–3 times smaller. The results from Ullrich17 were more consistent with satellite observations, which can be attributed to the enhanced deposition nucleation from the high dust concentrations. Our results demonstrated that the extremely high Nice could be fully explained by the heterogeneous nucleation of dust particles in DIBS. A conceptual framework is proposed to explain the dust‐cloud interactions in DIBS. This study confirms the significant dust effects on cirrus clouds in DIBS and highlights the importance of using aerosol‐aware ice nucleation parameterizations in modeling cirrus clouds when the dust is present.
Martian Proton Aurora Brightening Reveals Atmospheric Ion Loss Intensifying
Authors: He Fei , Fan Kai , Hughes Andréa , Wei Yong , Cui Jun , Schneider Nicholas , Fraenz Markus , Yao Zhonghua , Rong Zhaojin , Chai Lihui , Yan Limei , Wu Shi‐Qi , Zhang Xiao‐Xin 
Published: March 2023

AbstractThe Martian proton aurora is a distinct aurora phenomenon resulting from the direct deposition of solar wind energy into Mars' dayside atmosphere. What solar wind parameters influence the aurora activity in the short term is yet unknown, as are the associated repercussions in the Martian atmospheric ion loss. Here we present observational evidence of synchronized proton aurora brightening and atmospheric ion loss intensifying on Mars, controlled by solar wind dynamic pressure, using observations by the Mars Atmosphere and Volatile Evolution spacecraft. The solar wind dynamic pressure possibly has a saturation effect on brightening proton aurora. Significant erosion of the Martian ionosphere during periods of high dynamic pressure indicates at least five‐to‐tenfold increase in atmospheric ion loss. An empirical relationship between ion escape rate and auroral emission enhancement is established, providing a new proxy of Mars' atmospheric ion loss with optical imaging that may be used remotely and with greater flexibility.
Dentists' preparedness to provide Level 2 services in the North East of England: a mixed methods study
Authors: Simpson Sarah , Wallace Christopher K. , Smith Malcolm , Blaylock Paul , Vance Gillian 
Published: March 2023

AbstractBackground Dentists capable of delivering Level 2 complexity treatments may benefit patient access to dental care and increase workforce morale. However, little is known about attitudes, capability and training needs relating to Level 2 dental services.Aims To examine attitudes to, and perceived readiness for delivery of Level 2 dental services by NHS dentists in the North East of England and North Cumbria.Method A sequential mixed methods study with an online survey and semi-structured interviews. Participants were general, community, and hospital-based dental practitioners. Descriptive statistics of survey and thematic analysis of qualitative data were undertaken.Results Overall, 56% of 124 respondents had a limited understanding of the Level 2 performer role. A minority felt they were already providing Level 2 care across all speciality areas; 9% (n = 11) had a professional portfolio suitable for Level 2 accreditation. Confidence to undertake Level 2 competencies varied by speciality area, being highest in paediatric dentistry, and lowest in endodontics and orthodontics. Qualitative data identified motivations, and personal, organisational and system factors that were seen as barriers or enablers to upskilling.Conclusion The regional NHS workforce is unprepared to provide Level 2 dental services. Successful introduction will require review of necessary infrastructure and transparency of accreditation and contracting processes.
Drug_SNSMiner: standard pharmacovigilance pipeline for detection of adverse drug reaction using SNS data
Authors: Lee Seunghee , Woo Hyekyung , Lee Chung Chun , Kim Gyeongmin , Kim Jong-Yeup , Lee Suehyun 
Published: March 2023

AbstractAs society continues to age, it is becoming increasingly important to monitor drug use in the elderly. Social media data have been used for monitoring adverse drug reactions. The aim of this study was to determine whether social network studies (SNS) are useful sources of drug side effects information. We propose a method for utilizing SNS data to plot the known side effects of geriatric drugs in a dosing map. We developed a lexicon of drug terms associated with side effects and mapped patterns from social media data. We confirmed that well-known side effects may be obtained by utilizing SNS data. Based on these results, we propose a pharmacovigilance pipeline that can be extended to unknown side effects. We propose the standard analysis pipeline Drug_SNSMiner for monitoring side effects using SNS data and evaluated it as a drug prescription platform for the elderly. We confirmed that side effects may be monitored from the consumer’s perspective based on SNS data using only drug information. SNS data were deemed good sources of information to determine ADRs and obtain other complementary data. We established that these learning data are invaluable for AI requiring the acquisition of ADR posts on efficacious drugs.
Microalgal cultivation characteristics using commercially available air-cushion packaging material as a photobioreactor
Authors: Merz Clifford R. , Arora Neha , Welch Michael , Lo Enlin , Philippidis George P. 
Published: March 2023

AbstractAir-cushion (AC) packaging has become widely used worldwide. ACs are air-filled, dual plastic packaging solutions commonly found surrounding and protecting items of value within shipping enclosures during transit. Herein, we report on a laboratory assessment employing ACs as a microalgal photobioreactor (PBR). Such a PBR inherently addresses many of the operational issues typically encountered with open raceway ponds and closed photobioreactors, such as evaporative water loss, external contamination, and predation. Using half-filled ACs, the performance of microalgal species Chlorella vulgaris, Nannochloropsis oculata, and Cyclotella cryptica (diatom) was examined and the ash-free dry cell weight and overall biomass productivity determined to be 2.39 g/L and 298.55 mg/L/day for N. oculata, 0.85 g/L and 141.36 mg/L/day for C. vulgaris, and 0.67 g/L and 96.08 mg/L/day for C. cryptica. Furthermore, maximum lipid productivity of 25.54 mg/L/day AFDCW and carbohydrate productivity of 53.69 mg/L/day AFDCW were achieved by C. cryptica, while maximum protein productivity of 247.42 mg/L/day AFDCW was attained by N. oculata. Data from this work will be useful in determining the applicability and life-cycle profile of repurposed and reused ACs as potential microalgal photobioreactors depending upon the end product of interest, scale utilized, and production costs.
Automatic comprehensive aspects reports in clinical acute stroke MRIs
Authors: Liu Chin-Fu , Li Jintong , Kim Ganghyun , Miller Michael I. , Hillis Argye E. , Faria Andreia V. 
Published: March 2023

AbstractThe Alberta Stroke Program Early CT Score (ASPECTS) is a simple visual system to assess the extent and location of ischemic stroke core. The capability of ASPECTS for selecting patients’ treatment, however, is affected by the variability in human evaluation. In this study, we developed a fully automatic system to calculate ASPECTS comparable with consensus expert readings. Our system was trained in 400 clinical diffusion weighted images of patients with acute infarcts and evaluated with an external testing set of 100 cases. The models are interpretable, and the results are comprehensive, evidencing the features that lead to the classification. This system adds to our automated pipeline for acute stroke detection, segmentation, and quantification in MRIs (ADS), which outputs digital infarct masks and the proportion of diverse brain regions injured, in addition to the predicted ASPECTS, the prediction probability and the explanatory features. ADS is public, free, accessible to non-experts, has very few computational requirements, and run in real time in local CPUs with a single command line, fulfilling the conditions to perform large-scale, reproducible clinical and translational research.
A buprenorphine depot formulation provides effective sustained post-surgical analgesia for 72 h in mouse femoral fracture models
Authors: Wolter Angelique , Bucher Christian H. , Kurmies Sebastian , Schreiner Viktoria , Konietschke Frank , Hohlbaum Katharina , Klopfleisch Robert , Löhning Max , Thöne-Reineke Christa , Buttgereit Frank , Huwyler Jörg , Jirkof Paulin , Rapp Anna E. , Lang Annemarie 
Published: March 2023

AbstractAdequate pain management is essential for ethical and scientific reasons in animal experiments and should completely cover the period of expected pain without the need for frequent re-application. However, current depot formulations of Buprenorphine are only available in the USA and have limited duration of action. Recently, a new microparticulate Buprenorphine formulation (BUP-Depot) for sustained release has been developed as a potential future alternative to standard formulations available in Europe. Pharmacokinetics indicate a possible effectiveness for about 72 h. Here, we investigated whether the administration of the BUP-Depot ensures continuous and sufficient analgesia in two mouse fracture models (femoral osteotomy) and could, therefore, serve as a potent alternative to the application of Tramadol via the drinking water. Both protocols were examined for analgesic effectiveness, side effects on experimental readout, and effects on fracture healing outcomes in male and female C57BL/6N mice. The BUP-Depot provided effective analgesia for 72 h, comparable to the effectiveness of Tramadol in the drinking water. Fracture healing outcome was not different between analgesic regimes. The availability of a Buprenorphine depot formulation for rodents in Europe would be a beneficial addition for extended pain relief in mice, thereby increasing animal welfare.
Rapid imaging of thymoma and thymic carcinoma with a fluorogenic probe targeting γ-glutamyltranspeptidase
Authors: Yoshida Daisuke , Kamiya Mako , Kawashima Shun , Yoshioka Takafusa , Hino Haruaki , Abe Atsuki , Fujita Kyohhei , Kojima Ryosuke , Shinozaki-Ushiku Aya , Urano Yasuteru , Nakajima Jun 
Published: March 2023

AbstractIn recent years, thoracoscopic and robotic surgical procedures have increasingly replaced median sternotomy for thymoma and thymic carcinoma. In cases of partial thymectomy, the prognosis is greatly improved by ensuring a sufficient margin from the tumor, and therefore intraoperative fluorescent imaging of the tumor is especially valuable in thoracoscopic and robotic surgery, where tactile information is not available. γ-Glutamyl hydroxymethyl rhodamine green (gGlu-HMRG) has been applied for fluorescence imaging of some types of tumors in the resected tissues, and here we aimed to examine its validity for the imaging of thymoma and thymic carcinoma. 22 patients with thymoma or thymic carcinoma who underwent surgery between February 2013 and January 2021 were included in the study. Ex vivo imaging of specimens was performed, and the sensitivity and specificity of gGlu-HMRG were 77.3% and 100%, respectively. Immunohistochemistry (IHC) staining was performed to confirm expression of gGlu-HMRG's target enzyme, γ-glutamyltranspeptidase (GGT). IHC revealed high GGT expression in thymoma and thymic carcinoma in contrast to absent or low expression in normal thymic parenchyma and fat tissue. These results suggest the utility of gGlu-HMRG as a fluorescence probe for intraoperative visualization of thymomas and thymic carcinomas.
Input node placement restricting the longest control chain in controllability of complex networks
Authors: Alizadeh Samie , Pósfai Márton , Ghasemi Abdorasoul 
Published: March 2023

AbstractThe minimum number of inputs needed to control a network is frequently used to quantify its controllability. Control of linear dynamics through a minimum set of inputs, however, often has prohibitively large energy requirements and there is an inherent trade-off between minimizing the number of inputs and control energy. To better understand this trade-off, we study the problem of identifying a minimum set of input nodes such that controllabililty is ensured while restricting the length of the longest control chain. The longest control chain is the maximum distance from input nodes to any network node, and recent work found that reducing its length significantly reduces control energy. We map the longest control chain-constraint minimum input problem to finding a joint maximum matching and minimum dominating set. We show that this graph combinatorial problem is NP-complete, and we introduce and validate a heuristic approximation. Applying this algorithm to a collection of real and model networks, we investigate how network structure affects the minimum number of inputs, revealing, for example, that for many real networks reducing the longest control chain requires only few or no additional inputs, only the rearrangement of the input nodes.
Potential anti-proliferative activity of Salix mucronata and Triticum spelta plant extracts on liver and colorectal cancer cell lines
Authors: Ahmad Ghada M. , Abu Serie Marwa M. , Abdel-Latif Mohamed S. , Ghoneem Tayseer , Ghareeb Doaa A. , Yacout Galila A. 
Published: March 2023

AbstractCancer’s etiology is linked to oxidative stress. As a result, it's vital to find effective natural antioxidant remedies. Salix mucronata and Triticum spelta plant extracts were prepared using five different solvents and examined for their cytotoxicity against liver HepG2 cancer cell line. It was found that Salix mucronata ethanolic extract is high in antioxidant mediated anti-cancer activity. The functional constituents (phenolic and flavonoids) as well as preparation of different ethanolic concentrations used to study their properties that include DPPH, oxygen, hydroxyl, nitrogen radical scavenging activities, ferric reducing power and metal chelating activities. The MTT assay was used to determine antioxidant-mediated anti-cancer activity against human liver (HepG2) and colorectal (Caco-2) cancer cells to calculate the half-maximal growth inhibitory concentration (IC50). Moreover, flow cytometry analysis was used to quantify the apoptotic effect on the treated cancer cells. Additionally, qRTPCR of p53, BCL2, Cyclin D, MMP9 and VEGF were measured. Furthermore, HPLC was used to assess the most effective ingredients of the plant extract. Salix mucronata 50% ethanol extract had the highest polyphenolic content, anti-oxidant, and anti-proliferative activity. Salix mucronata increased the number of total apoptotic cells, and caused an upregulation of p53 gene expression by more than five folds and a downregulation of gene expression level of BCL2, Cyclin D, MMP9 and VEGF by more than five folds. Consequently, that could modulate oxidative stress and improve the effectiveness of cancer therapy. Results, also, showed that Triticum spelta ethanolic extract was less effective than Salix mucronata. Therefore, Salix mucronata ethanolic extract represents promising surrogate natural therapy for apoptosis-mediated cancer and recommended for further investigation using animal model.
Generation of a recombinant temperature-sensitive influenza D virus
Authors: Ishida Hiroho , Murakami Shin , Kamiki Haruhiko , Matsugo Hiromichi , Katayama Misa , Sekine Wataru , Ohira Kosuke , Takenaka-Uema Akiko , Horimoto Taisuke 
Published: March 2023

AbstractInfluenza D virus (IDV) is a causative agent of the bovine respiratory disease complex (BRDC), which is the most common and costly disease affecting the cattle industry. For developing a candidate vaccine virus against IDV, we sought to produce a temperature-sensitive strain, similar to the live attenuated, cold-adapted vaccine strain available against the influenza A virus (IAV). To this end, we produced a recombinant IDV (designated rD/OK-AL) strain by introducing mutations responsible for the adaptation of the IAV vaccine strain to cold conditions and conferring sensitivity to high temperatures into PB2 and PB1 proteins using reverse genetics. The rD/OK-AL strain grew efficiently at 33 °C but did not grow at 37 °C in the cell culture, indicating its high-temperature sensitivity. In mice, rD/OK-AL was attenuated following intranasal inoculation. It mediated the production of high levels of antibodies against IDV in the serum. When the rD/OK-AL-inoculated mice were challenged with the wild-type virus, the virus was not detected in respiratory organs after the challenge, indicating complete protection against IDV. These results imply that the rD/OK-AL might be a potential candidate for the development of live attenuated vaccines for IDV that can be used to control BRDC.
Measuring compound eye optics with microscope and microCT images
Authors: Currea John Paul , Sondhi Yash , Kawahara Akito Y. , Theobald Jamie 
Published: March 2023

AbstractWith a great variety of shapes and sizes, compound eye morphologies give insight into visual ecology, development, and evolution, and inspire novel engineering. In contrast to our own camera-type eyes, compound eyes reveal their resolution, sensitivity, and field of view externally, provided they have spherical curvature and orthogonal ommatidia. Non-spherical compound eyes with skewed ommatidia require measuring internal structures, such as with MicroCT (µCT). Thus far, there is no efficient tool to characterize compound eye optics, from either 2D or 3D data, automatically. Here we present two open-source programs: (1) the ommatidia detecting algorithm (ODA), which measures ommatidia count and diameter in 2D images, and (2) a µCT pipeline (ODA-3D), which calculates anatomical acuity, sensitivity, and field of view across the eye by applying the ODA to 3D data. We validate these algorithms on images, images of replicas, and µCT eye scans from ants, fruit flies, moths, and a bee.
Lactobacillus fermentumF40-4 ameliorates hyperuricemia by modulating the gut microbiota and alleviating inflammation in mice
Authors: Cao Jiayuan , Wang Ting , Liu Yisuo , Zhou Wei , Hao Haining , Liu Qiqi , Yin Boxing , Yi Huaxi 
Published: March 2023

L. fermentumF40-4 could inhibit UA synthetase activity in the liver to decrease UA production, promote expression level of UA excretion transporter NPT1 in the kidney and reshape microbiota in the gut to increase UA excretion, thereby alleviate HUA.
Exoplanet characterization using conditional invertible neural networks
Authors: Haldemann Jonas , Ksoll Victor , Walter Daniel , Alibert Yann , Klessen Ralf S. , Benz Willy , Koethe Ullrich , Ardizzone Lynton , Rother Carsten 
Published: March 2023

Context.The characterization of the interior of an exoplanet is an inverse problem. The solution requires statistical methods such as Bayesian inference. Current methods employ Markov chain Monte Carlo (MCMC) sampling to infer the posterior probability of the planetary structure parameters for a given exoplanet. These methods are time-consuming because they require the evaluation of a planetary structure model ~105times.Aims.To speed up the inference process when characterizing an exoplanet, we propose to use conditional invertible neural networks to calculate the posterior probability of the planetary structure parameters.Methods.Conditional invertible neural networks (cINNs) are a special type of neural network that excels at solving inverse problems. We constructed a cINN following the framework for easily invertible architectures (FreIA). This neural network was then trained on a database of 5.6 × 106internal structure models to recover the inverse mapping between internal structure parameters and observable features (i.e., planetary mass, planetary radius, and elemental composition of the host star). We also show how observational uncertainties can be accounted for.Results.The cINN method was compared to a commonly used Metropolis-Hastings MCMC. To do this, we repeated the characterization of the exoplanet K2-111 b, using both the MCMC method and the trained cINN. We show that the inferred posterior probability distributions of the internal structure parameters from both methods are very similar; the largest differences are seen in the exoplanet water content. Thus, cINNs are a possible alternative to the standard time-consuming sampling methods. cINNs allow infering the composition of an exoplanet that is orders of magnitude faster than what is possible using an MCMC method. The computation of a large database of internal structures to train the neural network is still required, however. Because this database is only computed once, we found that using an invertible neural network is more efficient than an MCMC when more than ten exoplanets are characterized using the same neural network.
Effect of Upper Lid Blepharoplasty Surgery on Corneal Topography and Corneal High-Order Aberrations
Authors: Ulas Mehmet Goksel , Tukenmez Dikmen Nejla 
Published: March 2023

AbstractThe objective of this study was to prospectively investigate the effect of upper eyelid blepharoplasty surgery for dermatochalasis on corneal topographic data and high-order aberrations (HOAs). Fifty eyelids of 50 patients with dermatochalasis who underwent upper lid blepharoplasty surgery were prospectively studied. A Pentacam (Scheimpflug camera, Oculus) device was used to measure corneal topographic values, astigmatism degree, and HOAs before and at 2 months after upper eyelid blepharoplasty. The mean age of the patients included in the study was 55.96 ± 12.4 years, 40 (80%) were female and 10 (20%) were male. We found no statistically significant difference in the pre-and postoperative values of corneal topographic parameters (p > 0.05 for all). In addition, we observed no significant postoperative change in the root mean square values of low, high, and total aberration values. In HOAs, we detected no significant change in spherical aberration, horizontal and vertical coma, and vertical trefoil; we only found a statistically significant increase in horizontal trefoil values after surgery (p < 0.05). In our study, we found that upper eyelid blepharoplasty did not cause significant changes in corneal topography, astigmatism, and ocular HOAs. However, studies are reporting different results in the literature. For this reason, it is important for patients considering upper eyelid surgery to be warned about visual changes that may occur after surgery.