bluuebunny/crossref_metadata_2025_split huggingface dataset:
A non-invasive nomogram predicting future lung metastasis in hepatocellular carcinoma: a multi-center cohort study
Authors: Huang Jianwen , Zheng Youbing , Wang Xiaofeng , Zhang Jie , Li Yong , Chen Xudong , Li Xiaoqun , He Xiaofeng , Duan Chongyang , Yan Jianfeng , Fu Sirui , Lu Ligong 
Published: April 2023

Abstract
Purpose Patients with hepatocellular carcinoma at higher risk of future lung metastasis should be identified for early diagnosis and treatments. Methods From 2006 to 2016, 352 multi-center cases were retrospectively reviewed and separated into training and validation datasets. Clinical factors and radiological parameters were used to construct models through combining backward stepwise hazard models with the least absolute shrinkage and selection operator method. Discrimination and calibration of both datasets were tested. We then subdivided patients according to our model and compared their time to lung metastasis and overall survival (OS). Multivariate regression analysis was used to determine whether subgroup was an independent factor for OS. Results The best model comprised maximum diameter, fusion lesions, ascites, alpha-fetoprotein level, and regional lymph node metastasis. The area under curve for predicting one-, two-. and three-year lung metastasis free survival were 0.78, 0.92, and 0.87 (training dataset), and 0.72, 0.72, and 0.71 (validation dataset), respectively, with sufficient calibration in both datasets. Subgroups separated according to the median score of best model showed significant differences in time to lung metastasis (training, p < 0.001; validation, p = 0.002) and OS (training, p < 0.001; validation, p < 0.001). Subgroupings were significant in multivariate regression for OS in both datasets: hazard ratio (HR) = 0.435 (95% confidence interval [CI]: 0.259–0.730), p = 0.002 in the training dataset; HR = 0.341 (95% CI: 0.178–0.653), p = 0.001 in the validation dataset. Conclusion Assisted by our model, patients at high risk of future lung metastasis could be identified. For high risk population, routine chest CT should be arranged, and more combination therapies should be explored.
Understanding the Impact of Public Charging Infrastructure on the Consideration to Purchase an Electric Vehicle in California
Authors: Hoogland Kelly , Kurani Ken , Hardman Scott , Chakraborty Debapriya 
Published: April 2023

Abstract
This research tests an implicit assumption in policies promoting public investment in plug-in electric vehicle (PEV) charging infrastructure: people who are not already interested in PEVs will see public PEV charging. Data from a survey representing all car-owning households in California are combined with data on public PEV charging and PEV registrations to estimate a structural equation model of the extent to which participants have considered acquiring a battery electric vehicle (BEV) or plug-in hybrid electric vehicle (PHEV), and whether participants report seeing PEV charging. The model controls for socio-economic and demographic measures and participants’ awareness, knowledge, and assessments of PEVs. The model also controls for the known spatial correlation between PEV registrations and public charging locations. An extension is made, via logistic ordinal regression modeling, to further assess whether charger density in the vicinity of a workplace affects the results. All results contradict the assumption that people will see charging infrastructure and that more charging—rather than more people seeing charging—means more people will consider purchasing a BEV or PHEV. Rather, prior interest, i.e., awareness, knowledge, and positive assessments of PEVs, allow people to see PEV charging. In short, interest in PEVs is a prerequisite to people seeing PEV charging.
Risk factor analysis of hemorrhagic cystitis (HC) after allogeneic hematopoietic stem cell transplantation in children with severe thalassemia
Authors: Dou Hui-Hong , Luo Jian-Ming , Zhao Yan-Jun , Wang Ji-Gan , Qin Yuan-Han 
Published: April 2023

Abstract
Purpose To explore the possible risk factors for hemorrhagic cystitis (HC).Methods We retrospectively analyzed 152 cases of severe thalassemia in allogeneic hematopoietic stem cell transplantation from January 2011 to December 2021 in our hospital. Evaluating incidence rate、occurrence time and duration of HC. Analysis of age,the degree of blood type compatibility, HLA matching configuration,the amount of CD34 + cells, Cyclosporin A concentration, Whether complications such as infection, GvHD and other complications were combined, Classification of risk of thalassemia (pretransplant assessment), different types of transplantation, in which that degree of the iron overload before and aft transplantation is related to the occurrence of HC.Results The results showed that the incidence of HC after transplantation was 27.63%.The median time for the occurrence of HC is 21d, and the duration is 11d.Only one cases were early onset HC (EOHC), and the other 41 were delayed HC (LOHC).The incidence rate of 4 years of age or older children with HC (33.70%) is higher than the age < 4 years old children (16.67%).The difference was statistically significant (P = 0.021).Conclusion Iron overload may have an impact on the occurrence of HC.As the level of SF increased, the incidence of HC increased significantly.iron overload may have an effect on HC occurrence.With the increase of SF, the incidence of HC increased significantly, especially when SF was greater than 7969.84.Children younger than 4 years of age were more likely to deelop HC after transplantation.
Appropriate fluoroscopic angle intraoperation of C-arm X-ray for each curved surface of femoral neck wall: a cadaveric study
Authors: Zhu QiuLiang , Yu Xiang-ping , Ma Jun , Lin Fang , Chen Yun-Yun , Ruan Wen-Bin 
Published: April 2023

Abstract
Objective To detect the appropriate fluoroscopic angle of intraoperative C-arm X-ray based on precise femoral neck anatomy.
Methods The femoral neck was divided into the anterior, posterosuperior and posteroinferior surfaces. These surfaces and the coronal plane of the femur formed anterior surface coronal angle (ACA), posterosuperior surface coronal angle (PSCA) and posteroinferior surface coronal angle (PICA), respectively. The three angles of 32 dried femoral specimens were measured. The femoral neck wall attached with steel wire wind aluminum foil model and three Kirschner wires perforating femoral neck wall model were prepared. The C-arm was rotated every 5° to complete the 0° -180 ° fluoroscopy of each curved surface. 111 frames of images were obtained for each femoral specimen. The appropriate fluoroscopic angle of each surface was obtained, and the fluoroscopic images characteristics of Kirschner wire penetrating the femoral neck cortex on three appropriate fluoroscopic angles and the anteroposterior and lateral fluoroscopy were observed.
Results The femoral neck is irregularly cylindrical with the anterior surface is longer than the posteroinferior surface, and the posterosuperior surface is the shortest. The measurement data of ACA, PSCA and PICA were (31±4.589)°, (67.813±5.052)° and (168.688±3.206)°, respectively. The appropriate fluoroscopic angle of the anterior, posterosuperior and posteroinferior surface of the steel wire aluminum foil model were (30.781±5.464)°, (67.969±3.721)°, (167.813±4.319)°, respectively. And there was no significant difference with the measurement data of the corresponding surface coronal angles, P > 0.05. The model of wire penetrating the femoral neck wall showed that the Kirschner wire penetrating the femoral neck could not be fully exposed in the traditional anteroposterior and lateral view films. Increasing the appropriate fluoroscopic angle of 30°, 70° and 170° could clearly find the Kirschner wire penetrating the cortex.
Conclusion Traditional anteroposterior and lateral fluoroscopy cannot accurately show the true structure of femoral neck. Additional 30°, 70° and 170° fluoroscopy can accurately observe the fracture reduction quality of the anterior surface, posterosuperior surface and posteroinferior surface of the femoral neck and the damage to the corresponding cortical bone caused by internal fixation.
Level of evidence: level II
Remote Animal Experiments of "Tumai" Surgical Robot Based on 5G Technology
Authors: Hu Ming , Ma Shixun , Ma Yuntao , Jing WuTang , Deng Yuan , Gong Shiyi , Wang Xiaopeng , Zhang Leisheng , Zhang Min , Cai Hui 
Published: April 2023

Abstract
Objective The remote robotic surgery based on 5G technology will be the future trend. To explore the feasibility, safety and stability of the domestic "Tumai" surgical robot in remote animal experiments under a 5G network environment.Methods The domestic "Tumai" surgical robot was used to perform remote cholecystectomy on experimental pigs in the animal operating room of Gansu Provincial Hospital from six different locations under a 5G network environment. The network rate, network delay, debugging time, loading time, operation time, blood loss, adverse events and intraoperative complications were recorded during the experiment. SPSS 25.0 statistical software was used, and the results were expressed as mean ± standard deviation.Results The experiment lasted 15 days, with a cumulative round-trip distance of > 3000 km, and the farthest surgical distance was about 780 km. Six remote animal cholecystectomies were completed. The average network delay was 55.16 ± 25.33ms; the average commissioning time was 65.17 ± 13.75 min; the average loading time was 5.12 ± 1.60 min; the average manipulation time of the robot was 22 ± 5.40 min; the average total operation time was 44 ± 4.23 min, the average blood loss was 5.83 ± 7.36 ml and one weak intraoperative network adverse. There was one intraoperative event and no intraoperative complications.Conclusions Using a domestic "Tumai" surgical robot system in the 5G wireless network environment could safely and stably perform remote animal surgery, which provides a theoretical foundation for the next step of remote clinical surgery.
Clinical observation and intervention analysis of patients with severe thrombocytopenia induced by tirofiban
Authors: Jing Rui , Guo Shi-jie , Li Yu-ming 
Published: April 2023

Abstract
Background There have been individual cases of successful treatment of tirofiban-induced thrombocytopenia by immunosuppressive agents (glucocorticoids and/or immunoglobulin), but no relevant case-control studies. This is a single-center, retrospective, case‒control study to observe the clinical characteristics of patients with severe thrombocytopenia induced by tirofiban and the intervention effect of immunosuppressive agents.Method 29 patients with severe thrombocytopenia induced by tirofiban during hospitalization in the Cardiology Department of our hospital from September 2008 to September 2021 were retrospectively observed. The patients were divided into 2 groups according to age. The elderly group included patients aged ≥ 60 years, with a total of 18 patients; the nonelderly group included patients aged༜60 years, with a total of 11 patients. Furthermore, the patients were divided into 2 groups according to whether they took immunosuppressive agents.The differences between the groups were compared between the lowest value of platelet levels, the time required for the platelet count to return to the normal range(≥100×109/L) after treatment, and the proportion of various bleeding events.Results There was no significant difference in the baseline value, the lowest value of platelet count, or the time required for the platelet count to return to the normal range between the elderly group and the nonelderly group (p > 0.05). In the elderly subgroup, there was no significant difference in the baseline value and the lowest value of platelet count between the treatment group and the control group (p > 0.05). However, the time required for the platelet count to return to the normal range in the treatment group was significantly shorter than that in the control group (p < 0.05). There were no significant differences in the various bleeding events between the elderly patients and nonelderly patients, or between the immunosuppressant treatment group and the control group in both elderly and nonelderly patients (p > 0.05).Conclusion immunosuppressive agents significantly shorten the time to normalization of the platelet count in elderly patients with tirofiban-induced severe thrombocytopenia; the bleeding risk in patients with tirofiban-induced severe thrombocytopenia does not increase with age.
Limitation of molecular twisting: Upgrading a donor-acceptor dye to drive H2 evolution
Authors: Zhu Kaijian , Rodríguez Ainoa Paradelo , Brands Maria , de Haas Titus , Buda Francesco , Reek Joost , Mul Guido , Huijser Annemarie 
Published: April 2023

Abstract
The performance of dye-sensitized photoelectrochemical (DSPEC) cells is currently hampered by the low efficiency of the photocathode, predominantly due to ineffective charge separation. To promote efficiency, donor-acceptor (D-A) dyes for photosensitization of the p-type semiconductor have been designed, spatially separating electrons and holes. We further improve on the state of the art by manipulating photoinduced twisting of a D-A P1 dye adsorbed onto NiO by co-adsorption of myristic acid, which has a carboxyl anchoring group and a long apolar alkyl chain. Time-resolved photoluminescence and Density Functional Theory studies show that twisting lowers the energy levels of the photoexcited D-A dye, while twisting is inhibited in case myristic acid is co-adsorbed on the NiO surface. The presence of myristic acid also favors light-induced charge separation, as apparent from femtosecond transient absorption, and increases the apparent photocurrent. Very interestingly, only in the presence of myristic acid light-induced H2 evolution is observed in aqueous media, despite the absence of a H2 evolution catalyst. We assign the H2 generation to a synergetic effect of inhibited twisting of the D-A dye radical anion increasing its electrochemical potential, combined with charge transfer and conversion of H+ on the hydroxylated NiO surface. Our work illustrates the importance of understanding effects of photoinduced intramolecular twisting and demonstrates that control thereof offers a simple design approach for efficient solar fuel devices.
Adult-onset Transient Pseudohypoaldosteronism Secondary to Ureteral Cancer: A Case Report
Authors: Yamamoto Mari , Sakamoto Fumitoshi , Ikai Hiroki , Fujita Yoshiro 
Published: April 2023

Abstract
Background:Pseudohypoaldosteronism (PHA) is a disorder in which patients show resistance to aldosterone, and exhibit aldosterone deficiency-like symptoms despite excessive secretion of aldosterone hormone from the adrenal gland. Secondary PHA (described here as transient PHA), which is characterized by transient aldosterone resistance secondary to other conditions, is usually an infantile disease. We report a rare case of adult-onset transient PHA (TPHA).
Case presentation:
A 70-year-old man admitted to our hospital, presented with renal failure of unknown cause with hypotension. Normal anion gap (AG) metabolic acidosis with hyperkalemia, an electrolyte abnormality, was also observed. A computed tomography (CT) scan showed bilateral ureteral dilatation and hydronephrosis. Blood renin level was found to be 70.5 ng/mL/h (normal: 2.5–21.4 ng/mL/h) and aldosterone level was 678.2 pg/mL (normal: 36–240 pg/mL). Abnormally high levels of aldosterone suggested aldosterone resistance. Based on the above history, a diagnosis of TPHA was made. Bilateral stenting was performed to improve the hydronephrosis, and the symptoms associated with TPHA resolved immediately after the obstruction was released. Bilateral ureteral carcinoma was found to be the cause of hydronephrosis.
Conclusions: Though TPHA is predominantly a pediatric disease, our study shows TPHA can also occur in adults if there is a decrease in the filtration rate in the ureter with concomitant chronic inflammation. Aldosterone resistance and electrolyte abnormalities were quickly resolved as soon as the obstruction was released in the present case. This is the first report to show the changes in the hormone (renin and aldosterone) levels before and after lifting the aldosterone resistance through the release of ureteral obstruction. Thus, in cases of normal AG metabolic acidosis with hyperkalemia, pseudohypoaldosteronism should be considered during differential diagnosis, in addition, to type Ⅳ renal tubular acidosis.
Structures of LIG1 active site mutants reveal the importance of DNA end rigidity for mismatch discrimination
Authors: Gulkis Mitchell , Tang Qun , Petrides Matthew , CAGLAYAN MELIKE 
Published: April 2023

Abstract
ATP-dependent DNA ligases catalyze phosphodiester bond formation in the conserved three-step chemical reaction of nick sealing. Human DNA ligase I (LIG1) finalizes almost all DNA repair pathways following DNA polymerase-mediated nucleotide insertion. We previously reported that LIG1 discriminates mismatches depending on the architecture of the 3'-terminus at a nick, however the contribution of conserved active site residues to faithful ligation remains unknown. Here, we comprehensively dissect the nick DNA substrate specificity of LIG1 active site mutants carrying Ala(A) and Leu(L) substitutions at Phe(F)635 and Phe(F)F872 residues and show completely abolished ligation of nick DNA substrates with all 12 non-canonical mismatches. LIG1EE/AA structures of F635A and F872A mutants in complex with nick DNA containing A:C and G:T mismatches demonstrate the importance of DNA end rigidity, as well as uncover a shift in a flexible loop near 5'-end of the nick, which causes an increased barrier to adenylate transfer from LIG1 to the 5'-end of the nick. Furthermore, LIG1EE/AA/8oxoG:A structures of both mutants demonstrated that F635 and F872 play critical roles during steps 1 or 2 of the ligation reaction depending on the position of the active site residue near the DNA ends. Overall, our study contributes towards a better understanding of the substrate discrimination mechanism of LIG1 against mutagenic repair intermediates with mismatched or damaged ends and reveals the importance of conserved ligase active site residues to maintain ligation fidelity.
Ichthyofauna of Delice (Kizilirmak) River
Authors: GÜL Göktuğ , ATASAĞUN Sibel 
Published: April 2023

Abstract

This study aimed to determine fish species in the Delice River, one of the main tributaries of the Kizilirmak River. The study was conducted at 22 stations between March 2019 and February 2020seasonally. An electrofisher device was used for fishing. The standard lengths of samples were measured in the field, a maximum of 40 samples of the species with a large number of individuals were selected and brought to the laboratory to determine morphometric and meristic characteristics and then other individuals were released back into the water. Sixteen species belonging to 9 families were found in Delice River; Cyprinidae (3) Barbus tauricus, Capoeta sieboldii, Capoeta tinca; Leuciscidae (4) Alburnoides freyhofi, Alburnus escherichii, Chondrostoma angorense, Squalius cephalus; Gobionidae (1) Pseudorasbora parva; Acheilognathidae (1) Rhodeus amarus; Cobitidae (1) Cobitis simplicispina; Nemacheilidae (3) Oxynoemachelius angorae, Oxynoemachelius brandtii, and Seminemacheilus lendlii; Aphaniidae (1) Anatolichthys marassantensis; Poeciliidae (1) Gambussia holbrooki, and Salmonidae (1) Onchorhynchus mykiss were identified. Nine endemic and two invasive species were identified in Delice River and its tributaries. It was determined that it would be beneficial to continue to monitoring studies for the future of the identified species.
The hallmarks of a tradeoff in transcriptomes that balances stress and growth functions
Authors: Dalldorf Christopher , Rychel Kevin , Szubin Richard , Hefner Ying , Patel Arjun , Zielinski Daniel , Palsson Bernhard 
Published: April 2023

Abstract

Fit phenotypes are achieved through optimal transcriptomic allocation. Here, we performed a high-resolution, multi-scale study of the transcriptomic tradeoff between two key fitness phenotypes, stress response (fear) and growth (greed), in Escherichia coli. We introduced twelve RNA polymerase (RNAP) mutations commonly acquired during adaptive laboratory evolution (ALE) and found that single mutations resulted in large shifts in the fear vs. greed tradeoff, likely through destabilizing the rpoB-rpoC interface. RpoS and GAD regulons drive the fear response while ribosomal proteins and the ppGpp regulon underlie greed. Growth rate selection pressure during ALE results in endpoint strains that often have RNAP mutations, with synergistic mutations reflective of particular conditions. A phylogenetic analysis found the tradeoff in numerous bacteria species. The results suggest that the fear vs. greed tradeoff represents a general principle of transcriptome allocation in bacteria where small genetic changes can result in large phenotypic adaptations to growth conditions.
Comparing the Effects of Jigsaw Cooperative Learning and Lecture on Health students' knowledge, performance, and satisfaction
Authors: Darabi Fatemeh , Karimian Zahra , Rohban Alireza 
Published: April 2023

Abstract
Background Jigsaw is a cooperative and student-centered learning method, emphasizing the constructive interactions of students and their active engagement in learning. The present research was conducted to compare the effects of two educational methods of traditional lecture and jigsaw cooperative learning (JCL) on the knowledge, performance, and satisfaction of students.
Methods This quasi-experimental study was done on 50 public health students with bachelor’s degrees, who had been chosen through available sampling. Eight educational sessions related to educational technology course were chosen for implementation of the study. One group of students received the education through the JCL, and the other through the lecture. The extent of performance and satisfaction of students with the educational course was measured through two researcher-made 80 and 18-item questionnaires after receiving the training. Data were analyzed through SPSS 23.0 software and P<05 was considered statistically significant.
Results The mean score of knowledge in the pre-test stage in the jigsaw and lecture groups was 11.40 and 10.56, respectively, while these values in the post-test stage significantly increased in the jigsaw group compared to their lecture counterpart (16.68 vs. 10.76) (P<0.0001). There was a significant difference between the two groups regarding the score of poster and pamphlet preparation, role-playing, and slide preparation in all subscales (P<0.0001). The mean score of satisfaction with the course was higher in the jigsaw group in all subscales except for motivation (P=0.17) and problem-solving (P=0.43), compared to the lecture group (P<0.0001).
Conclusions The results obtained from this research indicated that the JCL method, by engaging students in the teaching-learning process, could cause enhanced knowledge, performance, and satisfaction of the learner, helping them deal with their courses with greater interest. Thus, trainers and teachers should get familiar with this method and be encouraged to use it, since eventually use of this method leads to enhanced efficiency and quality of education in educational centers.