bluuebunny/crossref_metadata_2025_split huggingface dataset:
Does MPK4/12-HT1 function as a CO2/bicarbonate sensor to regulate the stomatal conductance under high CO2 Levels?
Authors: Gahlowt Priya , Tripathi Durgesh Kumar , Singh Samiksha , Gupta Ravi , Singh Vijay Pratap 
Published: August 2023

Abstract
Elevated atmospheric carbon dioxide (CO2) concentrations have a serious detrimental impact on both plants as well animals (Gojon et al. 2022). In particular, plants grown in high CO2 concentrations exhibit reduced contents of proteins, minerals, and nutrients (Ebi and Loladze 2019). The adverse effects of elevated CO2 have been reported on global food quality and quantity along with negative impacts on human health (Ebi and Loladze 2019; Jacobson et al. 2019). Therefore, identification and understanding of the CO2 sensing mechanisms in plants are of critical importance to avoid adverse consequences of high atmospheric CO2 levels.
Exploring the potential of Aloe-vera extract as a non-hazardous electrolytic solution for batteries
Authors: Kale Avinash , Jain Arihant , Kakde Satyam , Hede Krushna , Chopra Swamini , Pande Kavita , Deshmukh Abhay 
Published: August 2023

Abstract
Electrical energy plays a major role in our daily life and its consumption is also unlimited. There are various ways to store the electricity generated, most common of which is the use of acid batteries and portable dry batteries. The disposal of these batteries creates environmental hazard and are toxic to humans handling them as well. The aim of this research is to develop a non-hazardous, environment friendly battery using a non-conventional, botanical source: Aloe-vera plant. Aloe-vera leaves are known to contain acid, which has the potential to generate electric current. Thus, the potency of Aloe-vera extract was studied as an electrolytic solution to generate electricity through metal electrodes (Copper and Zinc), similar to a conventional acid battery. The pulp from Aloe-vera leaves was extracted in two different states; one with the outer cover intact and another was pure pulp from the inner layer. It was observed that though the initial voltage for both the states of Aloe-vera extract was same at 0.17 V, the voltage generated after 30 minutes of charging by pure pulp extract was approx. double than that of the extract with outer cover intact. In another iteration, both the types of Aloe-vera extract were mixed individually with concentrated sulphuric acid and distilled water and the output voltage was compared. It was noted that in all the iterations, the pure Aloe-vera pulp proved to be more efficient, while the addition of sulphuric acid gave highest voltage, ~ 33% more than pure pulp electrolyte. The future of this work is to develop a higher capacity battery and to scale down the present set-up.
Bioprospecting of Ten Microalgae Species Isolated from Saline Water Lake for Evaluation of the Biodiesel Production
Authors: Khosravinia Sara , Malekzadeh-Shafaroudi Saeid , bagheri Abdolreza , Behdad Assieh , Moshtaghi Nasrin 
Published: August 2023

Abstract
Algal bioprospecting in ecosystems leads to exploring native microalgae and the competency evaluation of economically producing lipids as biofuel or nutritional applications. In this study, ten microalgae species were screened from the saltwater lake. Chlorella vulgaris, Chlorella sorokiniana, Chlamydomonas raudensis, Chlamydomonas hedleyi, Dunaliella salina, Picochlorum bazangan sp. nov., Tetraselmis bazangan sp. nov., Haematococcus lacustris, Nannochloropsis oceanic, and Scenedesmu rubescens were isolated and identified using 18SrDNA and tufA markers. Biodiesel potentials were assayed by the determination of biomass productivity, biochemical components, fatty acid profile, and biodiesel properties. The results showed that the maximum biomass yield (1.22 gL-1) belonged to C. vulgaris. The highest protein, carbohydrate, chlorophyll, and carotenoid content were recorded in C. vulgaris, C. raudensis, C. sorokiniana, and D. salina, respectively. N. oceanica accumulated high lipid content and omega-3 fractions (31.09%). However, C. hedleyi had the highest lipid productivity (11.64 gL-1d-1) compared to other microalgae. The best species for biodiesel production was C. vulgaris, with a specific growth rate of 0.36d-1, lipid productivity of 7.45 gL-1d-1, and C16-C18 fatty acid profile of 78.3%. The microalgae C. vulgaris had appropriate biodiesel properties of low viscosity (4.49), high cetane number (55.38), and relatively low cloud point (4.98). Another choice was N. oceanic, with high lipid productivity, cetane number (59.79), oxidative stability (56.43), and low iodine value (47.11). Microalgae T. bazangan sp. nov. had a cetane number (55.24), low cloud point (4.71), and C16-C18 fatty acid profile of 82.34%. Accordingly, C. vulgaris, T. bazangan sp. nov., and N. oceanic can be considered potential species for biodiesel.
Median and Medial umbilical ligament repositioning for prevention of pelviperineal complications following abdominoperineal resection – A case series and novel technique
Authors: Thomas Anand , TS Subi , Sleeba Teena , Antony Abhijith , george Naveen 
Published: August 2023

Abstract
Introduction:
Pelviperineal complications after abdominoperineal resections are tough to treat. None of the available prophylactic methods has proven efficacy besides being technically challenging and expensive to perform. The present study aims to describe the technical details and short-term outcomes using mobilized umbilical ligaments to cover the pelvic inlet.
Technique:
After completing the rectal resection, the bladder with umbilical ligaments is mobilised anteriorly into the space of Retzius until the free edge can reach the sacral midline. Hitching stitches are taken to fix the umbilical ligaments into the new position. Seven consecutive patients had the umbilical ligament flap used for pelvic inlet closure.
Results
Cross-sectional imaging on day 30 demonstrated the viable flap in all patients, and the small bowel descent was prevented. None of the seven patients had small bowel obstruction till day 90 after the operation. No patient required re-catheterization, experienced major complications, or wound infections that would necessitate re-intervention.
Conclusion
The use of mobilised umbilical ligaments hitched to the pelvic inlet is a technically safe and feasible procedure to prevent pelviperineal complications after APR.
The Genomic Ghosts of Geukensia granosissima
Authors: Wares John P. 
Published: August 2023

Abstract
Observations that biodiversity - particularly in coastal marine species - is redistributing itself as the environment changes has been a key indicator of the climate change era. Historical surveys help us identify such patterns in taxa that might not be typically monitored for distribution or abundance. As recently as 1992, the southern ribbed mussel Geukensia granosissima was considered to be relatively abundant in northeastern Florida. Climate velocity predictions might suggest that these mussels would now have cryptically invaded the marshes of Georgia, which has historically only harbored the congener G. demissa, with possible ecosystem consequences. I re-surveyed Geukensia from both coasts of Florida and sites in Georgia for mitochondrial and nuclear genomic diversity. As of this report, G. granosissima is all but gone from the Atlantic coast of Florida, though some signature of its history remains in these data. The reason for this rapid fluctuation in distribution is not known, but is important for clarification of regional biogeographic patterns.
Gut resistome linked to Sexual Preference and HIV infection
Authors: Garcia Elisa Rubio , Casadellà Maria , Parera Mariona , Vila Jordi , Paredes Roger , Noguera-Julian Marc 
Published: August 2023

AbstractBackground:People living with HIV (PLWH) are at increased risk of acquisition of multidrug resistant organisms due to higher rates of predisposing factors. The gut microbiome is the main reservoir of the collection of antimicrobial resistance determinants known as the gut resistome. In PLWH, changes in gut microbiome have been linked to immune activation and HIV-1 associated complications. Specifically, gut dysbiosis defined by low microbial gene richness has been linked to low Nadir CD4+ T-cell counts. Additionally, sexual preference has been shown to strongly influence gut microbiome composition in PLWH resulting in differentPrevotellaorBacteroidesenriched enterotypes, in MSM (men-who-have–sex-with-men) or no-MSM, respectively. To date, little is known about gut resistome composition in PLWH due to the scarcity of studies using shotgun metagenomics. The present study aimed to detect associations between different microbiome features linked to HIV-1 infection and gut resistome composition.Results:Using shotgun metagenomics we characterized the gut resistome composition of 129 HIV-1 infected subjects showing different HIV clinical profiles and 27 HIV-1 negative controls from a cross-sectional observational study conducted in Barcelona, Spain. Most no-MSM showed aBacteroides-enriched enterotype and low microbial gene richness microbiomes. We did not identify differences in resistome diversity and composition according to HIV-1 infection or immune status. However, gut resistome was more diverse in MSM group,Prevotella-enriched enterotype and gut micorbiomes with high microbial gene richness compared to no-MSM group,Bacteroides-enriched enterotype and gut microbiomes with low microbial gene richness. Additionally, gut resistome beta-diversity was different according to the defined groups and we identified a set of differentially abundant antimicrobial resistance determinants based on the established categories.Conclusions:Our findings reveal a significant correlation between gut resistome composition and various host variables commonly associated with gut microbiome, including microbiome enterotype, microbial gene richness, and sexual preference. These host variables have been previously linked to immune activation and lower Nadir CD4+ T-Cell counts, which are prognostic factors of HIV-related comorbidities. This study provides new insights into the relationship between antibiotic resistance and clinical characteristics of PLWH.
Small size anterolateral bundle graft and decreased posterior slope angle of tibia are the risk factors associate with postoperative posterior tibial translation after double-bundle posterior cruciate ligament reconstruction
Authors: Morita Yudai , Tajima Takuya , Yamaguchi Nami , Yokoe Takuji , Nagasawa Makoto , Ota Tomomi , Ouchi Kouki , Chosa Etsuo 
Published: August 2023

Abstract
Posterior tibial translation (PTT) after double-bundle posterior cruciate ligament (PCL) reconstruction has sometimes occurred. Purpose of this study is to identify the risk factors for postoperative PTT after double-bundle PCL reconstruction with a hamstring autograft. Comparing the results of bilateral gravity sag view (GSV) at 12 months after surgery, over 5-mm PTT was defined as ‘failure’ in this study. Of 26 isolated PCL reconstruction cases, over 5-mm PTT was seen in 7 cases (group F: 9.57 ± 1.28 mm), and 19 cases had less than 5 mm (group G: 2.84 ± 1.29 mm). Age, sex, body mass index (BMI), preoperative GSV, posterior slope angle of the tibia, anterolateral bundle (ALB) and posteromedial bundle (PMB) graft diameters, and tibial tunnel diameter were evaluated. The two groups were compared with the 2x2 chi-squared test, the Mann Whitney U-test, and Spearman’s rank correlation coefficient. Multivariate logistic regression analysis was also performed to determine the risk factor. Statistical significance was indicated as p < 0.01 for correlation with postoperative PTT, and as p < 0.05 for all other comparisons. Mean age (group G 31.8 ± 12.5 vs group F 34.9 ± 15.9 years), sex (male/female: 15/4 vs 3/4), BMI (25.6 ± 4.6 vs 24.9 ± 3.9 kg/m2), preoperative GSV (11.3 ± 2.2 vs 11.6 ± 2.9 mm), PMB diameter (5.37 ± 0.33 vs 5.36 ± 0.48 mm), and tibial tunnel diameter (9.32 ± 0.58 vs 9.29 ± 0.49 mm) showed no significant differences. ALB diameter was significantly greater in group G (7.0 ± 0.5 mm) than in group F (6.5 ± 0.29 mm; p = 0.022). There was also a significant difference in posterior tibial slope angle (9.19 ± 1.94 vs 6.54 ± 1.45, p = 0.004). On Spearman rank correlation coefficient analysis, ALB diameter GSV (correlation coefficient: -0.561, p = 0.003) and posterior tibial slope angle (correlation coefficient: -0.533, p = 0.005) showed a significant correlation with postoperative PTT. Multivariate logistic regression analysis showed that ALB diameter (OR, 19.028; 95%CI, 1.082–334.6; p = 0.044) and posterior slope angle of tibia (OR, 3.081; 95%CI, 1.109–8.556; p = 0.031) were independently associated with postoperative PTT, respectively. In double-bundle PCL reconstruction with hamstring, smaller ALB graft diameter and lower (flatted) tibial slope angle were considered risk factors for postoperative PTT.
Research on Intelligent Recognition Technology in Lithology Based on Multi- parameter Fusion of Logging While Drilling
Authors: Liang Haibo , Xiong Jiaguo , Yang Yi , Zou Jialing 
Published: August 2023

Abstract
In oil and gas drilling, timely and accurate identification of formation lithology is an important guarantee of drilling safety. Aiming at the problems of inaccurate identification of lithology in drilling by traditional methods, and low efficiency due to the fact that even modern instruments cannot respond to lithology in real time. the Categorical Boost (CatBoost) model was applied to lithology identification in this study. However, since CatBoost uses more hyperparameters in its modeling, it is difficult to optimize model prediction by manually tuning the parameters. Therefore, the introduction of Kernel Principal Component Analysis (KPCA) extracts fewer and more important features from the original data, eliminates the redundant information contained therein, and combines with Bayesian Optimization (BO) algorithm to optimize the hyperparameters during the training process, thus improving the prediction performance of CatBoost. Two experiments were designed to verify the recognition ability of the model, and the final test results of the model showed that the KPCA-BO-CatBoost model proposed in this paper had the best overall performance, and the lithology recognition accuracy reached more than 90%. The model was effective in identifying the formation lithology, realized real-time lithology identification by combining the parameters of logging while drilling, improved the efficiency and accuracy of lithology identification, and was of great significance in guiding the subsequent drilling work.
Application of continuous nursing under the health belief model in prophylactic colostomy for rectal cancer
Authors: Sun Lin , Lu Jing , Wang Jiacheng , Zhang Hailing , Chu Aiqin 
Published: August 2023

Abstract
Background: To explore the effects of continuous nursing based on the health belief model on the rehabilitation of patients undergoing anterior rectal resection and preventive colostomy.
Methods: From September 2020 to October 2021, 80 patients who met the inclusion criteria for preventive enterostomy were randomly divided into the observation and control groups. Patients in both groups were followed up for 3 months after discharge from the hospital. The control group was given routine follow-ups, and the observation group was given continuous nursing intervention under the guidance of the health belief model. Adaptability to colostomy, colostomy self-efficacy, colostomy complications, quality of life after colostomy, and defecation control ability after colostomy were observed in the two groups.
Results: There were no significant differences in sex, age, tumor stage, tumor location, or educational level between the two groups (P > 0.05). Three months after intervention, colostomy adaptability and colostomy self-efficacy scores in the observation group were higher than those in the control group (132.38±8.33 and 101.26±9.4, respectively). The quality of life scores after colostomy in the observation group were higher than those in the control group at 1 month (49.2±6.99) and 3 months (59.85±5.16) after discharge, and the incidence of postoperative complications in the observation group was 20% lower than that in the control group (P < 0.05). The scores of defecation control ability at 1 month (21.25±11.05) and 3 months (17.475±9.808) after colostomy in the observation group were lower than those in the control group (P< 0.05).
Conclusion: Continuous nursing interventions based on the health belief model can improve colostomy adaptability, colostomy self-efficacy, quality of life, and defecation function after colostomy in patients with rectal cancer undergoing anterior rectal resection with prophylactic colostomy.
Mechanisms associated with the development of atrial fibrillation after sepsis and the role of neuregulin-1
Authors: Kang Wen , Deng Jingru , Fan Zheru , Zhou Fang , Wang Xi , Liu Kang , Wang Long 
Published: August 2023

Abstract
Purpose
To explore the mechanisms involved in the development of atrial fibrillation (AF) after sepsis and examine the effect of neuregulin-1 (NRG-1) on AF and related mechanisms.
Methods
We used cecal ligation and puncture (CLP) to establish the sepsis model. NRG-1 was administered via the tail vein at a dose of 0.01µg/g 12 and 24 h postoperatively to determine its effect on AF after sepsis.
Results
Compared with Sham rats, septic rats exhibited enhanced AF inducibility, atrial fibrosis, norepinephrine (NE), and C-reactive protein (CRP) levels, reduced action potential duration (APD), atrial effective refractive period (AERP), acetylcholine (Ach) levels, expression of Nav1.5, Cav1.2, and Kv1.5, and significantly decreased INa, ICa, L, and Ikur current densities. We observed that NRG-1 could reduce APD, atrial fibrosis, levels of CRP and NE, INa and ICa, L current densities, and expression levels of Nav1.5 and Cav1.2, however, it failed to prevent the onset of AF. Compared with the Sham group, the Sham + NRG-1 group rats showed a reduction in APD, AERP, INa and ICa, L current densities, Nav1.5 and Cav1.2 expression levels, elevated AF inducibility, Ach levels, Ikur current density, and Kv1.5 expression.
Conclusion
Sepsis can induce tissue and electrical remodeling in the atria and promotes the development of AF. NRG-1 could attenuate the degree of atrial fibrosis and organismal inflammation in sepsis while promoting the development of AF in Sham rats, impacting atrial electrophysiology and ionic currents.
"iNETgrate": integrating DNA methylation and gene expression data in a single gene network
Authors: Zare Habil , Sajedi Sogand , Ebrahimi Ghazal , Roudi Raheleh , Mehta Isha , Samimi Hanie , Kazempour Shiva , Zainulabadeen Aamir , Docking Thomas , Arora Sukeshi , Cigarroa Francisco , Seshadri Sudha , Karsan Aly 
Published: August 2023

Abstract
Integrating multi–omics data in one model can increase statistical power. However, designing such a model is challenging because different omics are measured at different levels. We developed the iNETgrate package (https://bioconductor.org/packages/iNETgrate/) that efficiently integrates transcriptome and DNA methylation data in a single gene network. Applying iNETgrate on five independent datasets improved prognostication compared to common clinical gold standards and a patient similarity network approach.
Green Synthesis of Nickel (Ni) Nanoparticles using Garlic Extract, Characterizations, and their Antimicrobial Activities
Authors: Kakar Natasha Saleem , Kasi Jafar , Bilal Ahmed , Akbar Ali , kasi Ajab , Ahmed Shehzad , Ali Syed Wajahat 
Published: August 2023

Abstract
The green synthesis route for production of nickel nanoparticles using garlic extract is being reported for first time. In the synthesis of Ni nanoparticles, garlic extract was used as reducing agent and nickel nitrate as a precursor salt and were characterized for their size, morphology, crystallinity and their effects on bacterial and fungal strains were investigated. Nanoparticles size was ranging from 20 to 130 nm and shapes were cylindrical and spherical confirmed by scanning electron microscope (SEM). X-ray diffraction (XRD) proved the crystalline nature and purity of nanoparticles. Ni nanoparticles were found effective against Escherichia coli, Salmonella typhi, Staphylococcus aureus and Klebsiella pneumoniae. The antibacterial activity was observed by measuring the diameter of inhibition zone using nanoparticles. Furthermore, nanoparticles have shown the potential to inhibit the growth of fungus such as Aspergillus nigerand Mucor mucedo. This novel antimicrobial potential is helpful for application in food industries and market.
Formal volunteering, informal volunteering and cognitive function among Chinese older adults
Authors: Liang Siya , Zhang Jiayu , Ye Zhicheng , Lan Ting , He Jiayi , Lin Wenyi 
Published: August 2023

Abstract
Background:
While the benefits of volunteering have been well-documented in the current literature, it does not tend to distinguish between various types of volunteering activities and the association between volunteering and cognitive function among Chinese older adults remains unclear. This study aims to examine association of volunteering and cognitive function among Chinese older adults and identify whether volunteering can be an effective intervention for dementia.
Method:
The 2018 China Health and Retirement Longitudinal Study (CHARLS) data were used for analysis. The generalized linear regression models are used to estimate the associations between volunteering type and cognitive function among Chinese older adults aged 60 and above (N=2370). Also, this study replaced the dependent variable to test the robustness of the regression results.
Results:
Informal volunteering does not significantly affect the cognitive function of Chinese older adults, while formal volunteering has a significant positive effect on Chinese older adults’ cognitive function. These findings are robust after replacing the dependent variable.
Conclusions:
This study provides evidence that formal volunteering can improve cognitive function among Chinese older adults, whereas informal volunteering can not, indicating that formal volunteering can be en effective intervention to protect against the decline of cognitive function. Therefore, formal volunteering should be incorporated into behavioral health intervention promgrams to help reduce the prevalence of cognitive impairment among Chinese older adults.
Molecular identification of Gekko gecko (black-spotted tokay geckoes) and its counterfeit based on mitochondrial 16S rRNA gene sequences
Authors: Wang Yilin , Jing Xiaotong , Chen Guangyu , Cai Bo , Zhao Chengjian , Li Hongyu , Huang Yong 
Published: August 2023

Abstract
Background
Authenticity identification has always been one of the hot research issues in the identification of traditional Chinese medicine. Since the Chinese medicinal materials are usually processed and stored dry, the DNA degradation is serious, and the standard DNA barcoding length (648bp) or longer gene fragments are difficult to amplify, which makes it impossible to conduct molecular identification of the Chinese medicinal materials. In this study, we used the mitochondrial 16S rRNA gene (< 200bp) as a barcode to identify Gekko gecko (black-spotted tokay geckoes) and its counterfeits. We collected a total of 63 samples from 17 species of Gekko gecko (black-spotted tokay geckoes) and its counterfeit, including not previously been involved in any molecular identification analyses.
Results
All samples were obtained 189 bp 16S rRNA sequences. The content of A + T (60%) was higher than that of G + C (40%). According to phylogenetic analysis and genetic distance, the genuine Gekko gecko (black-spotted tokay geckoes) samples collected in this study constitute a monophyly and can be distinguished from its counterfeits, including red-spotted tokay geckoes with very similar morphology.
Conclusions
Using the mitochondrial 16S rRNA gene can effectively identify Gekko gecko (black-spotted tokay geckoes) and its counterfeits, providing technical reference for the quality evaluation of Gekko gecko (black-spotted tokay geckoes) and clinical medication containing Gekko gecko (black-spotted tokay geckoes) components.