bluuebunny/crossref_metadata_2025_split huggingface dataset:
Evaluation of Gastrointestinal System Complaints and Comorbidities in Pediatric Familial Mediterranean Fever Patients
Authors: Karakas Hatice Dilara , Kuloglu Zarife , Aydin Fatma , Ozdemir Serpil , Avar-Aydin Pinar Ozge , Tuna Kirsaclioglu Ceyda , Cakar Nilgun , Ozcakar Zeynep Birsin 
Published: September 2023

Objective
Familial Mediterranean fever (FMF) is the most prevalent hereditary autoinflammatory disease among children. Abdominal pain and various gastrointestinal system (GIS) manifestations may arise directly from FMF or concomitantly with FMF. This study aimed to evaluate GIS complaints and findings other than classic peritonitis attacks in patients with FMF and to interpret concomitant GIS and hepatic disorders in these patients.

Methods
The medical and genetic findings of patients with FMF who attended our clinic between December 2011 and December 2021 were reviewed. Gastrointestinal system symptoms, liver function tests, abdominal images, and endoscopic and histopathological data were extracted from medical records.

Results
A total of 576 pediatric patients (female, 52.3%) diagnosed with FMF were included. Among them, almost one-fifth displayed GIS complaints, such as abdominal pain, defecation problems, and dyspepsia, distinct from typical FMF attacks. High serum aminotransferase levels were detected in 18.4% of the patients, with viral infections being the most common cause of moderate/severe hypertransaminasemia. In addition, during follow-up, 26.9% of them were referred to the pediatric gastroenterology department. At least 1 gastroenterological and hepatobiliary disorder was detected in 17.5% of the patients because of organic and functional GIS disorders or hepatobiliary disorders, such as gastroesophageal reflux disease, esophagitis, functional dyspepsia, and inflammatory bowel diseases.

Conclusion
Various GIS and hepatic disorders can be encountered in children with FMF. The spectrum of these complaints and pathologies can range from frequently observed health problems to more severe diseases.
HIV-Associated Rheumatic Diseases
Authors: Akram Bushra , Khan Meeshal , Humphrey Mary Beth 
Published: September 2023

Abstract
Human immunodeficiency virus (HIV) is widely prevalent among the world population. Although, historically, it has been linked to opportunistic infections in keeping with immunodeficiency and immune dysregulation, it has also been associated with a wide variety of autoimmune manifestations. With the introduction of highly active antiretroviral therapy and subsequent restoration of immunity, there have been multiple immune-mediated diseases that have resurfaced in the HIV population. Our review highlights autoimmune diseases in association with HIV and its targeted therapies in detail.
A 3 mm Port Reduces Postoperative Pain After Laparoscopic Colon Cancer Surgery: A Case-control Matched Study
Authors: Choi Jin Sun , Kim Hyo Jun , Lim Han-Ki , Kim Min Jung , Shin Rumi , Park Ji Won , Ryoo Seung-Bum , Park Kyu Joo , Park Hyeree , Shin Aesun , Jeong Seung-Yong 
Published: September 2023

Background:
Recently, smaller-size trocars and instruments have been developed for laparoscopic colon cancer surgery; however, their effectiveness and safety have not been elucidated. This study aimed to investigate whether 3 mm trocars and instruments have benefits compared with conventional trocars and instruments.

Patients and Methods:
Patients with colon cancer who underwent laparoscopic anterior resection or right hemicolectomy were included. Patients who underwent combined resections of other organs and those with conversion to open surgery were excluded. In the 3 mm group, three 5 mm trocars were replaced by 3 mm trocars. The numeric rating scale (NRS) immediately postoperatively at 24, 48, and 72 hours, respectively, after surgery and the use of additional analgesics and perioperative outcomes were analyzed. Case-control matched analysis was used to reduce bias according to the type of surgery.

Results:
A total of 207 patients (conventional: n = 158, 3 mm: n = 49) were included. Before matching, NRS 48 hours postoperatively (P = 0.049), proportion of patients using additional intravenous (IV) analgesics (P = 0.007), postoperative hospital stay (P < 0.001), and blood loss (P < 0.001) were lower in the 3 mm group. In multivariable analysis, trocar type significantly impacted the proportion of patients using additional IV analgesics (odds ratio: 0.330; 95% CI: 0.153-0.712; P = 0.005). After case-control matching, NRS immediately postoperatively (P = 0.015) and 24 hours postsurgery (P = 0.043), patients using additional IV analgesics (P = 0.019), postoperative hospital stay (P = 0.010), intraoperative blood loss (P < 0.001), and postoperative complication rate (P = 0.028) were significantly lower in the 3 mm group compared with the 5 mm group.

Conclusions:
The use of 3 mm trocars and instruments in laparoscopic colon cancer surgery can effectively reduce postoperative pain while maintaining perioperative safety.
Omentopexy/Gastropexy (OP/GP) Following Sleeve Gastrectomy Might be an Effective 2-in-1 Method (Reinforcement and Fixation): A Meta-Analysis of 14 Studies and a Call for Randomized Controlled Trials
Authors: Diab Abdul-Rahman Fadi , Alfieri Sarah , DeBlieux Paige , Williams Ashley , Docimo Salvatore , Sujka Joseph Adam , DuCoin Christopher Garnet 
Published: September 2023

Background:
Laparoscopic sleeve gastrectomy can lead to dangerous complications as leaks and hemorrhage. In addition, it can lead to gastric twist/torsion, prolonged postoperative nausea and vomiting (PONV), and de novo gastroesophageal reflux disease (GERD). We aimed to study the efficacy of omentopexy/gastropexy (OP/GP) in the prevention of these postoperative complications.

Materials and Methods:
PubMed and Google Scholar were queried in accordance with the Preferred Reporting Items for Systematic Reviews and Meta-Analyses guidelines. Data was analyzed using the Review Manager (RevMen) 5.4.1 software. Mantel-Haenszel statistical method and random effects analysis model were used in all meta-analyses. The odds ratio was used for dichotomous data. Subgroup analysis was done according to bougie size. Subgroup analysis according to the distance between the starting point of gastric transection and pylorus was not possible (limitation). Odds ratio and control event rate across studies were used to calculate the number needed to treat (NNT) with OP/GP for an additional beneficial outcome (prevention of adverse outcome) to occur.

Results:
The initial search identified 442 records; 371 were found irrelevant after screening and were excluded. The remaining 71 reports were retrieved and assessed for eligibility. An additional 57 reports were excluded following an in-depth assessment. The remaining 14 studies were included in this meta-analysis; 8 were nonrandomized studies (NRSs) while 6 were randomized controlled trials. Most studies originated from a single country (limitation). A statistically significant decrease in favor of OP/GP was observed for all outcomes (bleeding, leaks, gastric twist/torsion, prolonged PONV 1 month postoperatively, and postoperative de novo GERD). Data was consistent across studies (low I
2), and subgroup analysis according to bougie size revealed no subgroup differences. However, this study had 3 limitations that does not allow for strong conclusions.

Conclusions:
Although the current literature lacks strong scientific evidence, this study suggests that omentopexy/gastropexy (OP/GP) may offer protection against bleeding and leaks as a staple line reinforcement method, as well as against gastric twist/torsion, prolonged postoperative nausea and vomiting (PONV), and de novo gastroesophageal reflux disease (GERD) as a staple line fixation method. Therefore, it is worthwhile to proceed with large-scale, multicenter, randomized controlled trials to reevaluate our findings. Furthermore, conducting a comparison between OP/GP and other staple line reinforcement techniques would be beneficial.
Traditional Chinese medicine for age-related macular degeneration: A clinical evidence map between 2000 and 2022
Authors: Li Yamin , Liang Lina , Gao Yun , Xu Kai , Zhou Wei , Li Jiaxian , Jin Yu , Huang Ziyang , Li Xiaoyu 
Published: September 2023

AbstractBackgroundWe systematically searched and summarized clinical studies on traditional Chinese medicine (TCM) for age-related macular degeneration (AMD) with the method of evidence map. We aimed to provide evidence and research direction for the prevention or treatment of AMD with TCM in the future.Materials and methodsWe searched PubMed, Web of Science, EMBASE, and Chinese databases including CNKI (Chinese National Knowledge Infrastructure), WanFang and VIP, Clinical Trials.gov, and Chinese Clinical Trial Registry Center studies on the prevention or treatment of TCM for AMD from January 2000 to November 2022. We showed distribution features of these studies in the form of text description, graphs, and tables.ResultsThe final 258 articles included 245 clinical studies, 9 systematic reviews or meta-analyses, and 4 guidelines, expert consensus, or clinical pathway. After analyzing the articles with a focus on the stage of AMD, study type, sample size, intervention measures, outcome indicators, and the quality of evidence, we found that the studies were increasing, most of which were wet AMD, the main study type was randomized controlled trials, and the sample size was mostly fewer than 100 cases. Different interventions of TCM, such as Chinese medicine, Chinese patent medicine, or acupuncture, showed the potential benefits of improving vision, reducing fundus lesions, and alleviating some uncomfortable symptoms. However, the sample size of these studies was small. The outcome indicators were not unified, and the stage of AMD was unclear. The quality of articles was lower, and there were few studies on early AMD. The advantages of TCM for the prevention or treatment of AMD remained unclear.ConclusionsEarly intervention will be the focus and direction of AMD in the prevention or treatment of TCM, and more standardized clinical studies of TCM for early AMD need to be designed and carried out in the future.
Core mechanism and therapeutic methods of aging revealed by qi-collateral theory in traditional Chinese medicine
Authors: Li Hongrong , Hou Yunlong , Lu Xuan , Wang Lu , Li Mengnan , Kang Ning , Wu Yiling 
Published: September 2023

Abstract
Aging, a complex and multisystem process, has increasingly become a hot issue of global concern. Traditional Chinese medicine has accumulated rich understanding of aging during thousands of years of development, which provides valuable references for the in-depth study of overall aging. According to the qi-collateral theory in traditional Chinese medicine, essence is the origin of life; qi is the driving force of life; and spirit is the embodiment of life. This theory interprets key scientific issues such as the formation of life, the driving force of life, the function of zang-fu organs, and the spirit and consciousness. Furthermore, it reveals the key mechanism of aging. That is, the deficiency of kidney essence is the root of aging; the loss of vitality is the key of aging; and the depletion of body and spirit is the manifestation of aging. Based on the experience of medical practitioners in the past dynasties, such as nourishing essence, black food tonifying the kidney, and supplementing essence with flesh-blood products, the treatment method of invigorating kidney and supplementing essence, harmonizing yin and yang, reinforcing the vitality, and nourishing the body and spirit was proposed. Furthermore, an innovative Chinese patent medicine, Bazi Bushen capsules, is developed, which exerts the antiaging effect by replenishing qi, invigorating the spirit, and nourishing the body and spirit. Pharmacological and clinical studies have confirmed that this preparation has antiaging effects and are effective in the prevention and treatment of age-related diseases. Experimental studies have shown that Bazi Bushen capsules can delay the overall aging, reduce the DNA methylation and extend the healthy life of naturally aging mice, mitigate the age-related cognitive dysfunction, improve neurological function, maintain redox homeostasis and telomere integrity, and inhibit apoptosis. Moreover, Bazi Bushen capsules can increase the dermal thickness and the collagen fiber and elastic fiber content to prevent skin aging. In addition, Bazi Bushen capsules can correct the lipid metabolism disorders and ameliorate multisystem age-related diseases such as osteoporosis in surgically induced menopausal rats. This article discusses the pathogenesis, treatment, and prescription of aging and the antiaging theory, aiming to provide a basis for delaying aging and preventing related diseases.
A population-based study on hyperinsulinaemia and arterial stiffness in men with and without abdominal aortic aneurysm
Authors: Fatemi Shahab , Acosta Stefan , Zarrouk Moncef , Nilsson Peter M. , Gottsäter Anders 
Published: September 2023

Objectives
Patients with type 2 diabetes mellitus (DM) run lower risk for abdominal aortic aneurysm (AAA, aortic diameter ≥ 30 mm) and its complications. We aimed to evaluate associations between disturbances in glucose metabolism and arterial stiffness, AAA, and abdominal aortic diameter in 65-year-old men.

Methods
Forty-eight 65-year-old men with screening-detected AAA and 115 men with normal abdominal aortic diameter underwent examination of glucose metabolism and arterial stiffness.

Results
Men with AAA had higher BMI, waist-hip ratio (WHR), frequency of DM, haemoglobin A1c, smoking exposure, and plasma insulin levels at 0, 60 and 120 min during OGTT compared to those without. The increase in p-insulin (P < 0.001) after OGTT was also higher in men with AAA, adjusted for smoking, WHR, and nadir value of p-insulin. In analyses adjusted for smoking, use of lipid-lowering agents, and WHR, the increase in p-insulin at 2-hours (P = 0.006) after OGTT and p-homocysteine were associated with abdominal aortic diameter. There were no differences between groups in aortic stiffness or skin autofluorescence Advanced Glycation End products.

Conclusion
In this population-based study hyperinsulinaemia as a marker of insulin resistance, but not hyperglycaemia or aortic stiffness, was associated with AAA and abdominal aortic diameter in 65-year-old men.
Pseudomonas benzopyrenica sp. nov., isolated from soil, exhibiting high-efficiency degradation of benzo(a)pyrene
Authors: Dong Xingchen , Rao Zihuan , Wu Siyi , Peng Fang , Xie Zhixiong , Long Yan 
Published: September 2023

A Gram-negative, yellow-pigmented, aerobic and rod-shaped bacterium, designated as strain BaP3T, was isolated from the soil. Strain BaP3T grew at 16–37℃ (optimum, 30 °C) and pH 6.0–8.0 (optimum, pH 7.0). Additionally, strain BaP3T could tolerate NaCl concentrations in the range 0–6 % (optimum, 1%). Moreover, strain BaP3T was motile by flagella. The phylogenetic analysis of 16S rRNA sequences showed that strain BaP3T belonged to the genus

Pseudomonas

, and the sequence was most closely related to

Pseudomonas oryzihabitans

CGMCC 1.3392T and

Pseudomonas psychrotolerans

DSM 15758T, with 99.66 % sequence similarity.

Pseudomonas rhizoryzae

RY24T was the next closely related species, exhibiting 99.38 % 16S rRNA gene sequence similarity. The DNA–DNA hybridization and average nucleotide identity values between strain BaP3T and its closely related types were below 50 and 92 %, respectively. Both results were below the cut-off for species distinction. The genomic DNA G+C content of strain BaP3T was 65.30 mol%. The predominant quinone in strain BaP3T was identified as ubiquinone Q-9. The major cellular fatty acids were summed feature 8 (C18 : 1
ω7c and/or C18 : 1
ω6c), summed feature 3 (C16 : 1
ω7c and/or C16 : 1
ω6c) and C16 : 0. These results indicated that strain BaP3T represents a novel species in the genus

Pseudomonas

. The type strain is BaP3T (CCTCC AB 2022379T=JCM 35914T), for which the name Pseudomonas benzopyrenica sp. nov. is proposed.
Roles of Kdm6a and Kdm6b in regulation of mammalian neural regeneration
Authors: Yang Shu-Guang , Wang Xue-Wei , Li Chang-Ping , Huang Tao , Qian Cheng , Li Qiao , Zhao Ling-Rui , Zhou Si-Yu , Ding Chen-Yun , Saijilafu  , Liu Chang-Mei , Zhou Feng-Quan 
Published: September 2023

AbstractEpigenetic regulation of neuronal transcriptomic landscape is emerging to be a key coordinator of mammalian neural regeneration. Here we investigated roles of two histone 3 lysine 27 (H3K27) demethylases Kdm6a/b in controlling neuroprotection and axon regeneration. Deleting either Kdm6a or Kdm6b led to enhanced sensory axon regeneration in the PNS, whereas in the CNS only deleting Kdm6a in retinal ganglion cells (RGCs) significantly enhanced optic nerve regeneration. Moreover, both Kdm6a and Kdm6b functioned to regulate RGC survival but with different mechanisms. Mechanistically, Kdm6a regulates RGC regeneration via distinct pathway from that of Pten and co-deleting Kdm6a and Pten resulted in long distance optic nerve regeneration passing the optic chiasm. In addition, RNA-seq profiling revealed that Kdm6a deletion switched the RGC transcriptomics into a developmental-like state and suppressed several known repressors of neural regeneration. Klf4 was identified as a direct downstream target of Kdm6a-H3K27me3 signaling in both sensory neurons and RGCs to regulate axon regeneration. These findings not only revealed different roles of Kdm6a and Kdm6b in regulation of neural regeneration and their underlying mechanisms, but also identified Kdm6a- mediated histone demethylation signaling as a novel epigenetic target for supporting CNS neural regeneration.
Reentrant DNA shells tune polyphosphate condensate size
Authors: Chawla Ravi , Tom Jenna K. A. , Boyd Tumara , Grotjahn Danielle A. , Park Donghyun , Deniz Ashok A. , Racki Lisa R. 
Published: September 2023

ABSTRACTThe ancient, inorganic biopolymer polyphosphate (polyP) occurs in all three domains of life and affects myriad cellular processes. An intriguing feature of polyP is its frequent proximity to chromatin, and in the case of many bacteria, its occurrence in the form of magnesium-enriched condensates embedded in the nucleoid, particularly in response to stress. The physical basis of the interaction between polyP and DNA, two fundamental anionic biopolymers, and the resulting effects on the organization of both the nucleoid and polyP condensates remain poorly understood. Given the essential role of magnesium ions in the coordination of polymeric phosphate species, we hypothesized that a minimal system of polyP, magnesium ions, and DNA (polyP-Mg2+-DNA) would capture key features of the interplay between the condensates and bacterial chromatin. We find that DNA can profoundly affect polyP-Mg2+coacervation even at concentrations several orders of magnitude lower than found in the cell. The DNA forms shells around polyP-Mg2+condensates and these shells show reentrant behavior, primarily forming in the concentration range close to polyP-Mg2+charge neutralization. This surface association tunes both condensate size and DNA morphology in a manner dependent on DNA properties, including length and concentration. Our work identifies three components that could form the basis of a central and tunable interaction hub that interfaces with cellular interactors. These studies will inform future efforts to understand the basis of polyP granule composition and consolidation, as well as the potential capacity of these mesoscale assemblies to remodel chromatin in response to diverse stressors at different length and time scales.
Biofluorescence reveals hidden patterns in chitons with implications to visual ecology
Authors: Grimaldi Guido G. , Guidi Raiane dos Santos , Jardim Jaime A. , Hirota Marina , Pessoa Daniel Marques Almeida , Horta Paulo Antunes 
Published: September 2023

AbstractBiofluorescence is apparently widespread in the tree of life. Fluorescence has the potential to contribute to the totality of light leaving an organism’s surface and will therefore circumscribe how an individual could be detected and discriminated by their visual predators. Here, we: (i) documented the first record of biofluorescence on polyplacophorans, (ii) spectrally characterized the biofluorescence on shells of living chitons, (iii) measured the colour patch pattern variation, (iv) separated the colour of their shells into their reflectance and fluorescence components, and (v) combined these data with field measurements to calculate perceptual distance in chromatic and achromatic contrasts based on the visual system of their major visually guided predators. We found a red biofluorescence that enhances the brightness of chiton shells, along with a correlation showing that as individuals grow larger, the fluorescing surface area becomes smaller. Our visual models suggest that fluorescence decreases the achromatic contrast of chitons against their naturally fluorescent substrates for most visual predators, making them less noticeable to specific predators. Our results support the potential visual functionality of biofluorescence and open new hypotheses regarding its ecological roles to further investigations.
Safety Assessment of a Standardized Polyherbal Formulation (Glubloc™): Acute Toxicity Study and 28-Day Repeated Dose Toxicological Studies
Authors: Mayasa Vinyas , Raghuveer Pathuri , Chintamaneni Pavan Kumar , Gali Chaitanya Chakravarthi , Neerudu Uttam Kumar 
Published: September 2023

AbstractThe standardized extract ofMorus albaandMalus domestica Peelis widely recognized as a dietary supplement with potential benefits for blood sugar management, cholesterol reduction, and weight management. This study aimed to evaluate the general toxicity of the extract. Toxicity studies were conducted by OECD guidelines 425 and 407 and Schedule Y of the Drugs and Cosmetics Act, which mandates the use of two species for such studies. A single dose of GlublocTMat a concentration of 2000 mg/kg body weight was administered to Sprague Dawley rats and Albino mice, and no fatalities were reported, indicating good tolerability. Additionally, repeated oral administration of GlublocTMto rats and rabbits for a maximum duration of 28 days, at doses up to 414.16 mg/kg body weight per day in rats and 207.08 mg/kg body weight per day in rabbits, did not result in significant changes in medical assessments, ocular evaluations, weight gains, feeding behavior, or pathological findings compared to the control group. Overall, this study suggests that GlublocTMis well-tolerated without inducing any toxicity. The extract’s No-Observed-Adverse-Effect Level (NOAEL) is considered to be 414.16 mg/kg per day when administered repeatedly for 28 days in rats and 207.08 mg/kg in rabbits.
An effectiveC. elegansCRISPR training module for high school and undergraduate summer research experiences in molecular biology
Authors: Sandoval Carmen Herrera , Borchers Christopher , Aoki Scott Takeo 
Published: September 2023

ABSTRACTEngaging in research experiences as a high school or undergraduate student interested in science, technology, engineering, and mathematics (STEM) is pivotal for their academic and professional development. A structured teaching framework can help cultivate a student’s curiosity and passion for learning and research. In this study, an effective eight-week training program has been created that encompasses fundamental molecular biology principles and hands-on laboratory activities. This curriculum focuses on using clustered regularly interspaced short palindromic repeats (CRISPR) gene editing in theCaenorhabditis elegansmodel organism. Through pre- and post-program assessments, substantial enhancements in students’ molecular biology proficiency and enthusiasm for scientific exploration was observed. Overall, this diligently crafted training module that employsC. elegansas an educational tool to instruct inexperienced students has demonstrated its accessibility and ability to engage students in molecular biology and gene editing methodologies.