bluuebunny/crossref_metadata_2025_split huggingface dataset:
Unified bursting strategies in ectopic and endogenouseven-skippedexpression patterns
Authors: Berrocal Augusto , Lammers Nicholas C , Garcia Hernan G , Eisen Michael B 
Published: February 2023

AbstractTranscription often occurs in bursts as gene promoters switch stochastically between active and inactive states. Enhancers can dictate transcriptional activity in animal development through the modulation of burst frequency, duration, or amplitude. Previous studies observed that different enhancers can achieve a wide range of transcriptional outputs through the same strategies of bursting control. For example, despite responding to different transcription factors, alleven-skippedenhancers increase transcription by upregulating burst frequency and amplitude while burst duration remains largely constant. These shared bursting strategies suggest that a unified molecular mechanism constraints how enhancers modulate transcriptional output. Alternatively, different enhancers could have converged on the same bursting control strategy because of natural selection favoring one of these particular strategies. To distinguish between these two scenarios, we compared transcriptional bursting between endogenous and ectopic gene expression patterns. Because enhancers act under different regulatory inputs in ectopic patterns, dissimilar bursting control strategies between endogenous and ectopic patterns would suggest that enhancers adapted their bursting strategies to theirtrans-regulatory environment. Here, we generated ectopiceven-skippedtranscription patterns in fruit fly embryos and discovered that bursting strategies remain consistent in endogenous and ectopiceven-skippedexpression. These results provide evidence for a unified molecular mechanism shapingeven-skippedbursting strategies and serve as a starting point to uncover the realm of strategies employed by other enhancers.
Metabolic handoffs between multiple symbionts may benefit the deep-sea bathymodioline mussels
Authors: Zvi-Kedem Tal , Vintila Simina , Kleiner Manuel , Tchernov Dan , Rubin-Blum Maxim 
Published: February 2023

AbstractBathymodioline mussels rely on thiotrophic and methanotrophic chemosynthetic symbionts for nutrition, yet, secondary heterotrophic symbionts are often present and play an unknown role in the fitness of the organism. The bathymodiolineIdasmussels that thrive in gas seeps and on sunken wood in the Mediterranean Sea and the Atlantic Ocean, host at least six symbiont lineages that often co-occur, including the primary, chemosynthetic methane- and sulfur-oxidizing gammaproteobacteria, and the secondary Methylophagaceae, Nitrincolaceae and Flavobacteraceae symbionts, whose physiology and metabolism are obscure. Little is known about whether and how these symbionts interact or exchange metabolites. Here we curated metagenome-assembled genomes ofIdas modiolaeformissymbionts and used genomecentered metatranscriptomics and metaproteomics to assess key symbiont functions. The Methylophagaceae symbiont is a methylotrophic autotroph, as it encoded and expressed the ribulose monophosphate and Calvin-Benson-Bassham cycle enzymes, particularly RuBisCO. The Nitrincolaceae ASP10-02a symbiont likely fuels its metabolism with nitrogen-rich macromolecules and may provide the holobiont with vitamin B12. The FlavobacteriaceaeUrechidicolasymbionts likely degrade glycans and may remove NO. Our findings indicate that these flexible associations allow for expanding the range of substrates and environmental niches, via new metabolic functions and handoffs.
Genomic Analysis ofBlomia tropicalisIdentifies Novel Allergens for Component-Resolved Diagnosis of Mite Allergy
Authors: Xiong Qing , Liu Xiaoyu , Wan Angel Tsz-Yau , Malainual Nat , Xiao Xiaojun , Cao Hui , Tang Man-Fung , Ng Judy Kin-Wing , Shin Soo-Kyung , Sio Yang Yie , Wang Mingqiang , Sun Baoqing , Leung Ting-Fan , Chew Fook Tim , Tungtrongchitr Anchalee , Tsui Stephen Kwok-Wing 
Published: February 2023

AbstractBackgroundBlomia(B.)tropicalis, as an important species of house dust mites (HDMs), plays a critical role in allergic diseases in tropical populations, but its allergen components are less investigated than those of other HDMs. Multiple omics methods have largely improved the identification of mite allergens. Here, we sought to identify a comprehensive allergen profile ofB. tropicalisand advance the allergen component-resolved diagnosis (CRD) of mite allergy.MethodsReference mite allergen sequences were searched in a high-quality genome ofB. tropicalis. Comparative analysis was performed for important allergen groups. ELISA was used to assess the allergenicities of recombinant proteins of specific allergens.ResultsA complete allergen profile ofB. tropicaliswas revealed, including thirty-seven allergen groups (up to Blo t 42). In-depth comparative analysis not only determined the homology of major allergen groups 5 and 21 but also shed light on the emergence and divergence of chitin-binding allergens. The specific Blo t 12 was identified to be a chitin-binding protein originating from the chitinase of allergen group 15. Immunoassays of recombinant proteins verified three novel allergens and the ELISA results suggested geographical differences in theB. tropicalissensitization rate.ConclusionsThe comprehensive allergen profile revealed inB. tropicalis, the comparative analysis of allergen groups and the immunoassay assessment of recombinant proteins largely expanded our knowledge toB. tropicalisallergens and could ultimately benefit the CRD of HDM allergy.
Exploring the black box of human resource and business strategy linkage: the effect of strategic fits on organizational performance
Authors: Boy Akdag Leyla , Ekmekci Özge Tayfur 
Published: February 2023

PurposeThis study explores the effect of the strategic fit (tight-fit, minimal-fit and non-fit) between business strategies and human resource (HR) practices on perceived organizational performance (POP). It also investigates the moderating role of firm size on strategic fit–performance linkage.Design/methodology/approachThe data were gathered via an online survey from HR managers of companies listed in “Fortune-500 Turkey, ISO-Top and Second-Top 500”. The form was distributed to 669 organizations, and 102 of them responded. The data were analyzed using one-way ANOVA and moderation analysis.FindingsNo statistically significant difference was found in organizational performance according to different strategic fit categories. The moderating effect of firm size was not significant. Yet, tight-fit and minimal-fit appear to be linked to higher organizational performance. The results reveal that business strategies aligned with HR practices could have a favorable impact on organizational performance.Originality/valueThe study differs from previous studies in terms of methodology, the conceptualization of strategic fit categories, the nature of the sample and non-Western origin.
Global retailers' CSR initiatives during COVID-19 crisis: a cross-cultural examination
Authors: Ahmad Wasim , Battisti Enrico , Akhtar Naeem , Ahmad Muhammad Ishfaq , Rehman Ramiz Ur 
Published: February 2023

PurposeThis study develops a conceptual framework to examine (1) whether global retailers' CSR actions in the form of in-kind charitable contribution affect consumers' attribution of CSR to intrinsic motives, (2) whether consumers' attribution of CSR to intrinsic motives affects brand-self connection and (3) whether the cultural factors of self-transcendence and conservation moderate the relationship between consumers' attribution of CSR to intrinsic motives and brand-self connection.Design/methodology/approachData are collected from two culturally diverse countries, the US and China, each of which managed the pandemic in different ways. Before hypothesis testing, the invariance of measures is established. To measure differences between the groups, a multi-group analysis is conducted.FindingsGlobal retailers' in-kind charitable contribution is a significant drivers of consumers' attribution of CSR to intrinsic motives, and attribution of CSR to intrinsic motives has a positive effect on consumer brand-self connection. Both of the cultural values, self-transcendence and conservation, moderate the relationship between attribution of CSR to intrinsic motives and brand-self connection. Self-transcendence is a strong moderator in China, whereas conservation moderates strongly in the US. All of the relationships differ significantly between the groups (US versus China).Originality/valueTo the best of our knowledge, this is the first study to investigate empirically the role of in-kind charitable contribution in creating an attribution of CSR to intrinsic motives that eventually leads to strong brand-self connection in the COVID-19 context. The study provides novel insights into how consumer behavior differs across two significantly different cultures with regard to COVID-19-related CSR. The findings help international marketers manage uncertainties and crisis and to design their CSR-based marketing programs and develop positioning strategies across cultures.
Order matters: effect of use versus outreach order disclosure on persuasiveness of sponsored posts
Authors: Zhang Jin , Li Xinmai , Wu Banggang , Zhou Liying , Chen Xiang 
Published: February 2023

PurposeA critical step in influencer marketing is influencer outreach, where a brand reaches out to an influencer and forms a partnership. Yet little is known about how factors related to this process might influence the outcomes of sponsored posts. To address this gap, the authors investigated whether, how and when the order of influencers' product use and brand outreach (i.e. use/outreach order) affects post persuasiveness.Design/methodology/approachThe authors conducted three experimental studies. Studies 1 and 2 examined the effect of disclosure type (use-first, outreach-later vs. outreach-first, use-later vs. no disclosure) on consumers' responses to the post. Study 3 investigated the moderating effects of compensation disclosure type.FindingsThe results revealed that when the influencer used the product before (vs. after) being contacted by the brand, consumers had more favorable attitudes about the product and greater purchase intention upon reading the sponsored posts; perceived information diagnosticity mediated this effect. However, this tendency was mitigated if the influencer disclosed the specific monetary payment from the brand.Originality/valueThis research advances understanding of sponsorship disclosure and provides a way to manage its impact on message persuasiveness.
More humility for leaders, less procrastination for employees: the roles of career calling and promotion focus
Authors: He Wei , Zhang Zheng , Guo Qian 
Published: February 2023

PurposeBased on the conservation of resources theory, the authors explore the relationship between humble leadership and employee procrastination by introducing career calling and promotion focus, and constructing a moderated mediation model aiming to reveal the influence of humble leadership on employee procrastination.Design/methodology/approachA total of 217 valid samples were obtained using a two-time point paired questionnaire. The proposed hypotheses were tested using hierarchical regression.FindingsHierarchical regression results indicated that humble leadership had a significant negative effect on employee procrastination. Career calling played a fully mediating role in humble leadership and employee procrastination. Promotion focus not only plays a positive moderating role between humble leadership and career calling but also moderates the mediating role of career calling.Practical implicationsManagers should pay attention to the cultivation of their own character of humility in the process of communicating with their subordinates, increase employees' career calling from various aspects to improve employees’ sense of meaning and value for their work and understand employees' situation for personalized management.Originality/valueThis study reveals for the first time the inhibitory effect of humble leadership on employee procrastination through the conservation of resources theory. This helps in expanding research on the antecedents of procrastination behavior and enriching research on the effects of implementing humble leadership. For this reason, the study contributes to the literature on humble leadership, employee procrastination and the conservation of resources theory.
Do financial constraints moderate the relationship between CEO compensation and firm performance: an emerging market evidence
Authors: Farooq Muhammad , Khan Muhammad Imran , Noor Amna 
Published: February 2023

PurposeThe current study aims to investigate the impact of firm performance on chief executive officer (CEO) remuneration in the context of an emerging market, i.e. Pakistan. Further, the interactive effect of financial constraints is investigated in the pay–performance relationship.Design/methodology/approachThe study's sample includes 173 non-financial firms listed on the Pakistan Stock Exchange. This study covers the years 2010–2019. The CEO compensation of the sample firms is measured in terms of salary and bonuses, perquisites and stock options paid to the CEO, whereas firm profitability is measured by return on assets, return on equity, Tobin's Q (Q) and earnings per share. The KZ Index measures the degree of financial constraint. The fixed effect model (FEM) and system GMM estimation techniques were used to conclude the study's findings. In addition, to test the robustness of the results, the authors computed the level of financial constraints using the WW Index.FindingsThe findings show that firm performance has a significant positive impact on CEO compensation in all profitability measures except Tobin's Q. Further financial constraints have a significant negative impact on CEO compensation. The interactive variables of FC with all profitability measures have a significant negative impact on CEO compensation.Originality/valueThis study examines the relationship between firm performance and CEO compensation. Furthermore, the current study expanded the analysis by incorporating the role of financial constraints in the pay–performance relationship, which has not previously been tested, particularly in the context of an emerging market.
Post-purchase behaviour triggers in branded mobile shopping apps
Authors: Dobre Costinel , Milovan Anca-Maria , Preda Gheorghe , Naghi Remus 
Published: February 2023

PurposeThis study aims to integrate the perspectives offered by TAM and related models, respectively, the theory of values, to examine the impact of branded mobile shopping apps perceived value dimensions on continuance and recommendation intention.Design/methodology/approachA quantitative research was conducted online on a sample of 459 Romanian consumers to investigate how various facets of the perceived value of mobile shopping apps of some fashion brands influence continuance intention and the intention to recommend mobile apps. Sample selection implied a mixed non-probability method, convenience sampling and snowballing method, and the research hypotheses were tested using structural equation modelling (SEM) and path analysis.FindingsThis study validates significant positive relationships between perceived ubiquity, app incentive, respectively, epistemic value of branded mobile shopping apps and continuance intention, and between perceived hedonic value, social value, continuance intention and the intention to recommend branded mobile apps.Originality/valueThe research provides a deeper understanding of the influence played by the perceived value of mobile shopping apps on the consumer post-purchase behaviour and takes into consideration the mediating role of continuance intention for the perceived value and recommendation intention relationship.
Context-based learning for BIM: simulative role-playing games for strategic business implementations
Authors: Özener Ozan Önder 
Published: February 2023

PurposeThis paper presents a set of instrumental case studies for the context-based learning of BIM in the milieu of knowledge-based practice in the AEC industry. The study aimed to examine students' actions and perspectives in a simulated learning environment for real-world BIM processes. The core intent was to provide an in-depth understanding of strategic and functional BIM implementation by synthesizing a suggestive pedagogical framework based on context-based learning approaches.Design/methodology/approachDerived from context-based approaches and experiential learning methods such as role-play, problem-based and active learning, the study involved a set of doctoral-level case studies. In a qualitative research study, these cases were devised and organized around industry-focused simulations on various levels of BIM implementation strategies.FindingsResults from the case studies and the student responses suggest that the comprehensive evaluation of real-world BIM implementation simulations facilitates a solid understanding of the value of BIM. The participation of industry professionals catalyzes the development of strategic and functional BIM competencies.Originality/valueThe study proposes a well-structured and replicable BIM learning framework based on context-based learning approaches. The novel framework is adaptive and flexible for BIM education. It can provide students with the necessary skills, strategic vision and professional competencies for innovative practices in the 21st-century AEC Industry. The simulative learning settings, including the evaluation rubrics and connected instructional methods, can be implemented and further developed for similar education efforts.
After Grenfell: accumulation, debris, and forming failure in London
Authors: Smith Constance 
Published: February 2023

AbstractThe Grenfell Tower fire in London in 2017 is popularly associated with a litany of failures: political, structural, moral, material. But while the official inquiry has sought to frame the fire as a discrete event, for local residents it is inextricable from accumulated histories of injustice and inequality. Years on, the fire still reverberates, its afterlife constellating with new narratives and politics. Drawing on literature on ruination and remains, as well as methodological theory, this essay examines how the form of failure is never pre‐known but must be made to appear. Through diverse encounters with the tower's debris – material and conceptual – failure is made to matter, acting both backwards and forwards in time. Reflecting on remainders and erasures at the tower site, as well as debates about the parameters of the inquiry, I explore how failure is continually recomposed, becoming both an object of knowledge and an instrument for its formation.
Effect of dialyzer geometry on coagulation activation in the extracorporeal circuit in maintenance hemodialysis patients: Prospective randomized trial
Authors: ElSayed Hesham , Sayed Khalid Samir , Gharib Mohamed Sary 
Published: February 2023

AbstractBackground and ObjectivesThe coagulation cascade is activated during hemodialysis (HD) due to interaction of blood with the dialysis circuit. There is a paucity of data on the effect of the physical structure of the dialyzers on coagulation activation during HD. We conducted this study to compare the effect of Helixone FX80 versus Platinum H4 dialyzers on coagulation activation during HD.MethodsTwenty patients on maintenance HD were enrolled in this randomized prospective crossover study. Each patient was dialyzed using Helixone FX80 and Platinum H4 dialyzers. Serum thrombin‐antithrombin complex (TAT) was measured before (T0h) and at the end (T4H) of HD.ResultsThe absolute changes of serum TAT were comparable with the two dialyzers (median [IQR]: 1.15 [0.65, 1.75] for Helixone FX80 vs. 1.15 [0.67, 2.05] for Platinum H4, p = 0.371).ConclusionHelixone FX80 and Platinum H4 dialyzers have similar effects on coagulation activation during HD.
Between interdependence and autonomy: Toward a typology of work design modes in the new world of work
Authors: Reiche B. Sebastian 
Published: February 2023

AbstractDespite the rapid pace with which the world of work has been transforming, our concept of work design—the content and organization of work tasks, activities, relationships, and responsibilities—has remained remarkably resistant to change. This shortcoming not only limits our theoretical understanding of work design but also constrains organizations' ability to sufficiently adapt to human resource management (HRM) needs in the new world of work. I review the principal categories of work design to theorize about a typology of work design modes and their inherent HRM configurations. The typology proposes four ideal‐typical modes—organization‐defined work design, self‐directed internal work design, formalized external work design, and self‐governing work design—that differ in their requisite degrees of work interdependence and work autonomy. In a second step, I exemplify the conceptual dimensions of the typology in relation to three organizations using the case study as illustrative convention. The typology has several implications for theory, practice, and future research on work design and HRM.
Opioid consumption and non‐opioid multimodal analgesic treatment in pain management trials after hip and knee arthroplasties: A meta‐epidemiological study
Authors: Pedersen Casper , Vilhelmsen Frej Juul , Laigaard Jens , Mathiesen Ole , Karlsen Anders Peder Højer 
Published: February 2023

AbstractBackgroundThe leading principle in peri‐operative pain management is multimodal analgesia, which reduces opioid requirements and associated adverse effects. Pragmatic pain trials should optimally test interventions in addition to multimodal non‐opioid analgesics and interventions to ensure clinical relevance and baseline levels of opioid consumption that reflect clinical settings. We aimed to investigate opioid consumption and use of non‐opioid analgesics administered adjunct to interventions in post‐operative pain trials after total hip and knee arthroplasty.MethodsA systematic literature search was conducted 7 January 2020 in The Cochrane Library's CENTRAL, PubMed, and EMBASE. Trials investigating analgesic interventions for post‐operative pain in adults undergoing total hip or knee arthroplasty were included. The primary outcome was the aggregated median 0–24 h post‐operative opioid consumption. Further, we assessed the use of paracetamol, non‐steroidal anti‐inflammatory drugs, gabapentinoids, high‐dose glucocorticoids, local infiltration analgesia and nerve blocks administered as co‐interventions equally to all participants. We assessed trends over time for all outcomes.ResultsOf 14,200 records, 570 trials were included. Median 0–24 h opioid consumption was 21 and 22 mg iv morphine equivalents in hip and knee arthroplasty trials, respectively. Meta‐regression showed no overall linear correlation between opioid consumption and publication year. The use of multimodal non‐opioid analgesia increased over time, though only 48% of trials published from 2010 to 2020 administered two or more non‐opioid analgesics. Applying more non‐opioid analgesics was associated with lower opioid consumption in intervention groups.ConclusionPost‐operative 0–24 h morphine consumption was median 21–22 mg. The demonstrated differences in non‐opioid multimodal analgesic regimens between research and clinical settings, can potentially diminish the demonstrated opioid‐sparing effects of trial interventions when such are implemented in a clinical context.