bluuebunny/crossref_metadata_2025_split huggingface dataset:
Manufacturing of ceramic venus figurine replica from a mold by combining traditional and digital technologies
Authors: Saha Sunita , Tomkowska Anna , Martusewicz Jacek , Sitnik Robert 
Published: February 2023

Abstract
In this paper, we propose a method for creating a ceramic Venus figurine replica from a mold in the Museum of Ceramic Techniques collection in Koło, Poland stored at the museum for many years. Moreover, none of the Venus figurines in this collection have been preserved to the present day. Our process starts with partially degraded mold fragments and ends with an actual copy of the Venus figurine in three forms: faience kilned to bisque, porcelain, and glazed faience. The entire process involves understanding old manufacturing technology, conservation supervision, and state-of-the-art 3D scanning, data processing, and 3D printing technologies. We began with a preservation state evaluation that included a technical analysis of the degradation state of the mold. Then, we applied 3D scanning technology using the structured light method. Later, we integrated all mold fragments into front and back 3D models. These 3D models were optimized for 3D printing technology and were used to manufacture the mold. Finally, the printed fragments were corrected using traditional techniques performed by art conservators. This process took comparatively less time and produced a more realistic result than would have been observed if only 3D software had been used. This process also enabled us to create educational materials for the Koło museum about old manufacturing technologies. From the point of view of museum visitors, the copies are made with sufficient detail that they are indistinguishable from handmade artifacts. Our estimates show that combining both techniques (traditional and digital) saved approximately 25% of the time that would have been used if the individual techniques had been used separately. We also believe that our use case could be transferred to a broader group of objects that use molding in their production processes.
Electroanalytical biosensor based on GOx/FCA/PEG-modified SWCNT electrode for determination of glucose
Authors: Han Do Kyoung , Li Cheng Ai , Song Sung Ho , Cho Kun , Choi Jong-Soon , Son Seong Eun , Seong Gi Hun 
Published: February 2023

AbstractThis paper describes a simple electrochemical sensing platform based on single-walled carbon nanotube (SWCNT) electrodes for glucose detection. The device fabrication using O2-plasma treatment allows precision and uniformity for the construction of three SWCNT electrodes on the flexible plastic substrate. Glucose assay can be simply accomplished by introducing a glucose sample into the fabricated biosensor. The marked electrocatalytic and biocompatible properties of biosensors based on SWCNT electrodes with the incorporation of ferrocenecarboxylic acid and polyethylene glycol enable effective amperometric measurement of glucose at a low oxidation potential (0.3 V) with low interferences from coexisting species. The device shows efficient electroanalytical performances with high sensitivity (5.5 μA·mM−1·cm−2), good reproducibility (CV less than 3%), and long-term stability (over a month). A linear range of response was found from 0 to 10 mM of glucose with a fast response time of 10 s. This attractive electroanalytical device based on GOx/FCA/PEG/SWCNT electrodes offers a promising system to facilitate a new approach for diverse biosensors and electrochemical devices.
Accuracy of keyless vs drill-key implant systems for static computer-assisted implant surgery using two guide-hole designs compared to freehand implant placement: an in vitro study
Authors: Raabe Clemens , Schuetz Tabea S. , Chappuis Vivianne , Yilmaz Burak , Abou-Ayash Samir , Couso-Queiruga Emilio 
Published: February 2023

Abstract
Purpose
This in vitro study aimed at comparing the accuracy of freehand implant placement with static computer-assisted implant surgery (sCAIS), utilizing a keyless and a drill-key implant system and two guide-hole designs.

Methods
A total of 108 implants were placed in 18 partially edentulous maxillary models simulating two different alveolar ridge morphologies. 3D digital deviations between pre-planned and post-operative implant positions were obtained. Guide material reduction was assessed in the keyless implant system for the manufacturer’s sleeve and sleeveless guide-hole designs.

Results
sCAIS using a sleeveless guide-hole design demonstrated smaller mean angular, crestal and apical deviations compared to sCAIS utilizing a manufacturer’s sleeve and the freehand group (2.6 ± 1.6°, vs 3.3 ± 1.9°, vs 4.0 ± 1.9°; 0.5 ± 0.3 mm, vs 0.6 ± 0.3 mm, vs 0.8 ± 0.3 mm; and 1.0 ± 0.5 mm, vs 1.2 ± 0.7 mm, vs 1.5 ± 0.6 mm). Smaller angular and apical mean deviations were observed in the keyless implant system as compared with the drill-key implant system (3.1 ± 1.7°, vs 3.5 ± 1.9°, p = 0.03; and 1.2 ± 0.6 mm, vs 1.4 ± 0.7 mm, p = 0.045). Overall, smaller angular, crestal, and apical deviations (p < 0.0001) were observed in healed alveolar ridges (2.4 ± 1.7°, 0.5 ± 0.3 mm, and 0.9 ± 0.5 mm) than in extraction sockets (4.2 ± 1.6°, 0.8 ± 0.3 mm, and 1.6 ± 0.5 mm). Higher mean volumetric material reduction was observed in sleeveless than in manufacturer’s sleeve guide-holes (− 0.10 ± 0.15 mm3, vs − 0.03 ± 0.03 mm3, p = 0.006).

Conclusions
Higher final implant positional accuracy was observed in sCAIS for the keyless implant system, with a sleeveless guide-hole design, and in healed ridges. Sleeveless guide holes resulted in higher volumetric material reduction compared with the manufacturer’s sleeve.
Report of the online meeting of the 7th Asian Conference on Environmental Mutagens (ACEM), China, November 5-6, 2022
Authors: Zhang Rui , Chen Wen 
Published: February 2023

AbstractThe 7th Asian Conference on Environmental Mutagens (ACEM 2022) was held online from November 5–6, 2022. However, the 19th Chinese Environmental Mutagen Society Meeting was postponed due to the pandemic prevention policies of COVID-19 and the time will be announced later. In total, 467 participants from 8 countries, including China, Japan, Korea, Philippines, etc. participated in the virtual conference. Eight keynote speakers and 16 lecturers in 2 symposia made their speeches online on topics aligned with the theme “The Impact of Global Change on Asian Environment and Genomic Health”. More than 270 abstracts were submitted in this conference. We sincerely appreciate the efforts of all the participants, organizers, and members from Asian Association of Environmental Mutagen Societies (AAEMS). ACEM 2022 was a success and provided an excellent platform for exchanging the latest developments and stimulating scientific collaboration in the Asia-Pacific region as well as other parts of the world.
Automated synthesis and quality control of [68Ga]Ga-PentixaFor using the Gaia/Luna Elysia-Raytest module for CXCR4 PET imaging
Authors: Daniel Thomas , Balouzet Ravinet Clara , Clerc Jérôme , Batista Rui , Mouraeff Yvan 
Published: February 2023

Abstract
Background
[68Ga]Ga-PentixaFor is a promising radiotracer for positron emission tomography imaging of several human tumors overexpressing the chemokine receptor-4 (CXCR4). CXCR4 overexpression has been demonstrated in patients with hematologic malignancies, solid cancers, as well as cardiovascular pathologies of inflammatory origins. However, its radio synthesis is not yet fully developed in France, and existing methods do not use our type of synthesis module. Therefore, we aimed at developing a [68Ga]Ga-PentixaFor synthesis with Gaia/Luna Elysia-Raytest module to use it in clinical purpose.

Results
12 syntheses were carried out by varying the temperature conditions and radiolabeling times, and led to choose specific labelling conditions with the Gaia/Luna Elysia-Raytest module: 97 °C, 4 min. The mean synthesis time of the 3 validation runs under good manufacturing practice (GMP) was 24 min 27 s (± 8 s), and the mean radiochemical yield was 87.0% [standard deviation (SD) 6.67%]. Different quality control parameters were also evaluated in accordance with European Pharmacopeia: radiochemical and radionuclidic purity, pH, sterility, stability and endotoxins levels. The average radiochemical purity was 99.1% (SD 0.25%) assessed by instant thin layer chromatography and 99.8% (SD 0.092%) assessed by high pressure liquid chromatography. average [68Ge] breakthrough was 1.48 × 10–5%, under the recommended level of 0.001%. We assessed the stability of the radiotracer up to 4 h at room temperature (no augmentation of the [68Ga] chloride in the final product, i.e. radiochemical purity (RCP) > 98.5%). The endotoxins levels were < 5 EU/mL, and the pH was 6.5 (same for the three syntheses).

Conclusion
The [68Ga]Ga-PentixaFor synthesis process developed on the Gaia/Luna Elysia-Raytest module has fulfilled all acceptance criteria for injectable radiopharmaceutical products regarding the European Pharmacopeia. The radiochemical purity, stability, efficacy, as well as the microbiological quality of the three GMP batches were found to be good. The robustness of the synthesis process may be suitable for multi-dose application in clinical settings.
The recombination regulator RecX negatively regulates heat-stable antifungal factor (HSAF) biosynthesis in Lysobacter enzymogenes
Authors: Li Kaihuai , Hou Rongxian , Zhou Xue , Xiong Chunlan , Li Cheng , Wang Yong , Liu Fengquan 
Published: February 2023

AbstractBacteria often use multiple transcription factors to regulate specific biological processes. Biosynthesis of heat-stable antifungal factor (HSAF) is regulated by multiple factors in Lysobacter enzymogenes. However, the mechanism of HSAF biosynthesis regulation remains largely unknown. In this study, we screened a potential HSAF biosynthesis regulator, RecX, by a DNA pull-down assay. Deletion of recX resulted in a significant increase in the production of HSAF, and overexpression of recX significantly suppressed HSAF production. Importantly, our results showe that RecX directly binds to the promoter region of the lafB gene to inhibit its transcription and thus decreases HSAF production in L. enzymogenes. These findings reveal the novel mechanism of RecX regulation of antifungal antibiotic production in L. enzymogenes.
Biochar-promoted methane production and mitigation of acidification during thermophilic anaerobic co-digestion of food waste with crude glycerol: comparison with re-inoculation
Authors: Li Xiaojue , Shimizu Naoto 
Published: February 2023

Abstract
Food waste and crude glycerol were anaerobically co-digested for 100 days at 52 ± 1 °C with an organic loading rate of 1.0 g L−1 d−1. This long-term thermophilic anaerobic digestion (AD) system encountered severe inhibition from volatile fatty acids (VFAs). The study investigated the impacts of re-inoculation (RI) and biochar addition (BA) on this AD process, and monitored the variation of pH, VFAs, total alkalinity and total ammonia nitrogen during treatment. RI treatment was effective in the short term by recovering reactivity after inhibited sludge was mixed 1:1 with active inoculant. In the long term, RI could not reverse process imbalance and finally failed on day 56. Superior performance in methane production and process stability was observed in BA reactors when compared with control and RI reactors. Overall, the biochar contributed to alkalinity and facilitated the activation of methanogenesis and stimulated the conversion of VFAs.
Survival, Durable Tumor Remission, and Long-Term Safety in Patients With Advanced Melanoma Receiving Nivolumab
Authors: Topalian Suzanne L. , Sznol Mario , McDermott David F. , Kluger Harriet M. , Carvajal Richard D. , Sharfman William H. , Brahmer Julie R. , Lawrence Donald P. , Atkins Michael B. , Powderly John D. , Leming Philip D. , Lipson Evan J. , Puzanov Igor , Smith David C. , Taube Janis M. , Wigginton Jon M. , Kollia Georgia D. , Gupta Ashok , Pardoll Drew M. , Sosman Jeffrey A. , Hodi F. Stephen 
Published: February 2023

Purpose Programmed cell death 1 (PD-1) is an inhibitory receptor expressed by activated T cells that downmodulates effector functions and limits the generation of immune memory. PD-1 blockade can mediate tumor regression in a substantial proportion of patients with melanoma, but it is not known whether this is associated with extended survival or maintenance of response after treatment is discontinued. Patients and Methods Patients with advanced melanoma (N = 107) enrolled between 2008 and 2012 received intravenous nivolumab in an outpatient setting every 2 weeks for up to 96 weeks and were observed for overall survival, long-term safety, and response duration after treatment discontinuation. Results Median overall survival in nivolumab-treated patients (62% with two to five prior systemic therapies) was 16.8 months, and 1- and 2-year survival rates were 62% and 43%, respectively. Among 33 patients with objective tumor regressions (31%), the Kaplan-Meier estimated median response duration was 2 years. Seventeen patients discontinued therapy for reasons other than disease progression, and 12 (71%) of 17 maintained responses off-therapy for at least 16 weeks (range, 16 to 56+ weeks). Objective response and toxicity rates were similar to those reported previously; in an extended analysis of all 306 patients treated on this trial (including those with other cancer types), exposure-adjusted toxicity rates were not cumulative. Conclusion Overall survival following nivolumab treatment in patients with advanced treatment–refractory melanoma compares favorably with that in literature studies of similar patient populations. Responses were durable and persisted after drug discontinuation. Long-term safety was acceptable. Ongoing randomized clinical trials will further assess the impact of nivolumab therapy on overall survival in patients with metastatic melanoma.
Two Cases of Adrenal Malignancy With Macroscopic Fat
Authors: Wilkinson Tom , Hunt Penny , McHaffie Alexandra 
Published: February 2023

Abstract
The presence of macroscopic fat on computed tomography (CT) imaging has been traditionally regarded as an indication that an adrenal lesion is likely to be a benign myelolipoma, for which further investigation is not usually required. Two cases are described where an adrenal lesion was eventually found to be malignant on histology (adrenocortical carcinoma in the first case, undifferentiated sarcoma in the second case), despite the presence of macroscopic fat on CT. In both cases there were other clinical and radiological indicators of potential malignant pathology. These cases add to increasing awareness in the literature that malignant adrenal tumors may rarely contain macroscopic fat, emphasizing a need for clinical vigilance.
DESENVOLVIMENTO ORIENTADO POR MISSÕES SOB A ÓTICA DA CONSTITUIÇÃO ECONÔMICA BRASILEIRA
Authors: Fernandes Rodrigo Mineiro , Clark Giovani , Corrêa Leonardo Alves 
Published: February 2023

Objetivo: O artigo procura identificar quais seriam os pressupostos institucionais para a construção de um novo modelo de desenvolvimento que seja compatível com o discurso normativo da Constituição Econômica à luz de sua ideologia constitucionalmente adotada.Metodologia: A pesquisa, de natureza documental, seguiu o método analítico substancial a partir do referencial teórico da “ideologia constitucionalmente adotada”, desenvolvida pelo pioneiro do Direito Econômico brasileiro, Professor Washington Peluso Albino de Souza.Resultados: Conclui-se pela possibilidade de compatibilizar o modelo neoestruturalista das missões aos ditames da Constituição Econômica brasileira, dentro de um projeto novo-desenvolvimentista, considerando a pluralidade ideológica do texto constitucional e as alternativas para o desenvolvimento nacional.Contribuições: O modelo neoestruturalista orientado por missões apresenta-se como uma efetiva alternativa ao modelo neoliberal de austeridade para o desenvolvimento nacional, com foco na distribuição de renda e nos investimentos sociais, de forma a melhorar a vida da população brasileira, sendo totalmente compatível com os ditames constitucionais de planejamento, desenvolvimento, pluralismo produtivo e universalização da dignidade humana.
Exploring potential strategies to enhance memory and cognition in aging mice
Authors: Bhat M Shreevatsa , M G Ramesh Babu , Dhanushkodi Anandh , Rai Prof Kiranmai S 
Published: February 2023

Background: Aging population is rapidly expanding worldwide, and age-related cognitive impairments prove detrimental for achieving a better productive and quality of life. Lack of effective therapies for age-related cognitive impairment focuses attention on developing preventive strategies, such as nutritional interventions, cell therapies and environmental manipulations. The objective of the present study was to explore the comparative benefits of potential memory-enhancing strategies like supplementation of choline (Ch) and docosahexaenoic acid (DHA) or administration of human embryonic kidney stem cell conditioned media (HEK-CM) or exposure to environmental enrichment (EE), that attenuates cognitive impairments in aging mice. Methods: 12-month-old CF1 male mice were subdivided [n=6/group] into normal aging control (NAC), saline vehicle control (SVC), Ch-DHA, EE, heat-inactivated HEK-CM (HIHEK-CM) and HEK-CM mice. Spatial working and reference memory were assessed using an eight-arm radial maze test and cognition using a novel object recognition test (NORT). Results: Spatial memory and cognition were decreased in normal aging mice. Aged mice exposed to dietary Ch-DHA or HEK-CM showed significant enhancement in spatial learning tasks, memory and cognition compared to the same in age-matched NAC mice. Ch-DHA and HEK-CM treated mice committed significantly lesser reference memory errors and attained a higher percentage of correct choices in spatial learning and memory tasks. Moreover, on testing for cognition in NORT, significantly higher number of visits to the novel object was observed in Ch-DHA supplemented and HEK-CM administered aging mice whereas HEK-CM and EE mice groups showed significantly greater number of visits to familiar object, when compared to same in age-matched NAC and HIHEK-CM groups, respectively. Conclusion: Supplementation of Ch-DHA and HEK-CM treatment strategies have a higher potential [~ 20—30%] for enhancing spatial learning, memory and cognition in normal aged mice, whereas exposure to EE seems to enhance only their short-term memory.
New uses for vitamin C, and its versatile, pleiotropic antioxidant action. - Treatment of neoplasms, skin diseases, bone diseases and stimulation of the immune system
Authors: Adamus Justyna , Abram Kamila , Banaś Patryk , Pierzchała Jakub Rafał , Bednarz Katarzyna , Sobańska Natalia , Banasiak Aleksandra Paulina , Teichman Rafał , Kasprowicz Jakub , Hyjek Michał 
Published: February 2023

Introduction: Vitamin C (Vit C) is an organic chemical compound from the group of unsaturated polyhydroxy alcohols. It influences physiological processes such as the facilitation of iron absorption, hormone and carnitine synthesis, and is also involved in epigenetic processes. It is a vitamin, which means that it must be provided in food. It is also an antioxidant used as a food additive. In recent times, its pleiotropic use has been proven in the treatment of immune deficiencies, the treatment of skin diseases, bone diseases and may also be used in new cancer treatment regimens.

Aim of the study: To review the current literature on the use of vitamin C in various fields of health and science.

Materials and Methods: We reviewed the literature available in PubMed, using the key words: "vitamin C"; "ascorbid acid"; "cancer treatment"; "vitamin C immune function".

Results: Vitamin C has pleiotropic effects on the human body, both through its effects on cells and tissues and through its antioxidant mechanisms and regulation of gene expression. It has been shown to be a key antioxidant with anti-inflammatory and immune system supportive effects. Its effect of slowing down skin photo-ageing by reducing the amount of metalloproteinases induced by reactive oxygen species has been noted in the literature. Vitamin C has been shown in studies to reduce the risk of osteoporosis due to its osteoclast inhibitory properties. Beneficial effects of high-dose intravenous infusions have also been demonstrated in the treatment of cancer, as well as about reducing organ failure and vascular damage in patients with ARDS and sepsis.

Summary: The versatile action of vitamin C has made it the subject of intense research. However, the reports in the literature to date on its use require further studies on larger groups of people to confirm its effects.
Impact of adverse drug reactions on the outcomes of tuberculosis treatment
Authors: Sant´Anna Flávia M. , Araújo-Pereira Mariana , Schmaltz Carolina A. S. , Arriaga María B. , Andrade Bruno B. , Rolla Valeria C. 
Published: February 2023

Background
Adverse drug reactions (ADR) challenge successful anti-tuberculosis treatment (ATT). The aim of this study was to evaluate the impact of ATT-associated ADR and related factors on ATT outcomes.

Methods
A prospective cohort study of persons with tuberculosis (TB) at a referral center in Rio de Janeiro, Brazil, from 2010 to 2016. Baseline information: race, sex, schooling, economic status, tobacco, drugs and alcohol abuse, HIV-infection status and comorbidities were captured during TB screening and diagnosis. Laboratory exams were performed to confirm TB diagnosis and monitor ADRs, favorable (cure and treatment completion) and unfavorable (death, loss to follow up and failure) outcomes were prospectively captured. The Kaplan-Meier curve was used to estimate the probability of ADR-free time. A logistic regression analysis (backward elimination) was performed to identify independent associations with unfavorable outcomes.

Results
550 patients were enrolled, 35.1% were people living with HIV (PLHIV) and ADR occurred in 78.6% of all participants. Smoking (OR: 2.32; 95% CI:1.34–3.99) and illicit-drug use (OR:2.02; 95% CI:1.15–3.55) were independent risk factors for unfavorable outcomes. In PLHIV, alcohol abuse and previous ART use were associated with unfavorable outcomes. In contrast, ADR increased the odds of favorable outcomes in the overall population. PLHIV more frequently experienced grade 3/4-ADR (18.36%), especially “liver and biliary system disorders”. Lower CD4 counts (<100 cells/uL) were associated with hepatotoxicity (p = 0.03). ART-naïve participants presented a higher incidence of ADR in comparison with ART-experienced patients.

Conclusion
Substance use was associated with unfavorable outcomes, highlighting the need for better strategies to reduce this habit. In contrast, ADRs were associated with favorable outcomes. Attention to the occurrence of ADR in PLHIV is essential, especially regarding hepatotoxicity in those with high immunosuppression.