bluuebunny/crossref_metadata_2025_split huggingface dataset:
Empirical evidence of tourist satisfaction and loyalty in the case of Bucharest—A partial least squares structural equation modelling approach
Authors: Muntean Mihaela‐Carmen , Sorcaru Iulian Adrian , Manea Ludmila‐Daniela 
Published: January 2023

AbstractTourist satisfaction (TS), tourist loyalty (TL), the decision to revisit a destination and the choice to recommend it to acquaintances can have a decisive influence on inbound tourist flows. These influencing factors are closely related to tourist behaviour. The main objective of this study was to investigate TS and loyalty of both foreign and Romanian tourists in Bucharest, by testing under‐examined and new variables. The hypotheses and conceptual research model were based on the following antecedents and consequences of TS: tourists' expectations and travel motivations (TEM), destination authenticity and infrastructure quality, destination safety (DS), tourists' emotions (TE) and TL. The analysis was unique in testing the effect of novelty‐seeking tourists' desire to extend their stay on their expectations and motivations. The mediating effect of TE on the relationship between satisfaction and loyalty was also tested for the first time. Data were collected using a 24‐item online questionnaire. The data were modelled with partial least squares structural equation modelling. Based on 122 valid responses processed with SmartPLS software, the results confirm most of the research hypotheses. The findings reveal that tourists' desire to extend the length of stay on their expectations and motivations has the highest statistical contribution to TEM. Furthermore, a statistically insignificant relationship between DS and TS was identified. The findings are useful for all stakeholders of tourism development in Bucharest.
Cinnamaldehyde Ameliorates Dextran Sulfate Sodium‐Induced Colitis in Mice by Modulating TLR4/NF‐κB Signaling Pathway and NLRP3 Inflammasome Activation
Authors: Tan Xiaofen , Wen Yifan , Han Zhijun , Su Xuyang , Peng Jing , Chen Feng , Wang Yadong , Wang Tianming , Wang Changzhong , Ma Kelong 
Published: January 2023

AbstractUlcerative colitis (UC) is a chronic inflammatory gastrointestinal disease mainly associated with immune dysfunction and microbiota disturbance. Cinnamaldehyde (CIN) is an active ingredient of Cinnamomum cassia with immunomodulatory and anti‐inflammatory properties. However, the therapeutic effect and detailed mechanism of CIN on UC remains unclear, and warrant further dissection. In this study, network pharmacology and molecular docking analyses were introduced to predict the potential targets and mechanism of CIN against UC. The therapeutic effect and the predicted targets of CIN on UC were further validated by in vivo and in vitro experiments. Seven intersection targets shared by CIN and UC were obtained, and four hub targets, i. e., toll‐like receptor 4 (TLR4), transcription factor p65 (NF‐κB), NF‐kappa‐B inhibitor alpha (IκBα), prostaglandin G/H synthase 2 (COX2) were acquired, which were mainly involved in NF‐κB, tumor necrosis factor (TNF), Toll‐like receptor and NOD‐like receptor signaling pathways. CIN alleviated the symptoms of dextran sulfate sodium (DSS)‐induced colitis by decreasing the disease active index (DAI), restoring colon length, and relieving colonic pathology. CIN attenuated systemic inflammation by reducing serum myeloperoxidase (MPO), TNF‐α, interleukin‐6 (IL‐6), and interleukin‐1β (IL‐1β), down‐regulating TLR4, phosphorylated‐NF‐κB (p‐NF‐κB), phosphorylated‐IκBα (p‐IκBα), and COX2 expression in colonic tissues, and decreasing NOD‐like receptor protein 3 (NLRP3), Caspase‐1, and IL‐1β protein expression in lipopolysaccharide (LPS)‐stimulated RAW264.7 cells. These results indicate that CIN alleviates DSS‐induced colitis inflammation by modulating TLR4/NF‐κB signaling pathway and NLRP3 inflammasome activation.
Telltale atria—The meaning of adverse remodeling
Authors: Eng Marvin H. , Zahr Firas 
Published: January 2023

Key Points
Right atrial area index/left atrial area index ratio (RLR) above 0.6 was associated with higher mortality rates.
Elevated RLR are also associated with pulmonary hypertension.
Surgical data suggests that reverse atrial remodeling is possible and associated with improved outcomes.
In Vivo Applications of Bioorthogonal Reactions: Chemistry and Targeting Mechanisms
Authors: Mitry Madonna M. A. , Greco Francesca , Osborn Helen M. I. 
Published: January 2023

AbstractBioorthogonal chemistry involves selective biocompatible reactions between functional groups that are not normally present in biology. It has been used to probe biomolecules in living systems, and has advanced biomedical strategies such as diagnostics and therapeutics. In this review, the challenges and opportunities encountered when translating in vitro bioorthogonal approaches to in vivo settings are presented, with a focus on methods to deliver the bioorthogonal reaction components. These methods include metabolic bioengineering, active targeting, passive targeting, and simultaneously used strategies. The suitability of bioorthogonal ligation reactions and bond cleavage reactions for in vivo applications is critically appraised, and practical considerations such as the optimum scheduling regimen in pretargeting approaches are discussed. Finally, we present our own perspectives for this area and identify what, in our view, are the key challenges that must be overcome to maximise the impact of these approaches.
Breeding populations of Marbled Godwits and Willets have high annual survival and strong site fidelity to managed wetlands
Authors: Sandercock Brett K. , Gratto‐Trevor Cheri L. 
Published: January 2023

AbstractThe Prairie Pothole Region of central Canada supports a diverse community of breeding waterbirds, but many species have declining populations and the demographic mechanisms driving the declines remain unknown. We conducted a 7‐year field study during 1995–2001 to investigate the demographic performance of Marbled Godwits (Limosa fedoa) and Willets (Tringa semipalmata) breeding in managed wetlands near Brooks, Alberta. Mark‐recapture analyses based on Cormack–Jolly–Seber models revealed that the annual rates of apparent survival for Marbled Godwits ( = 0.953 ± 0.012SE) and Willets ( = 0.861 ± 0.015SE) are among the highest rates of survivorship reported for any breeding or nonbreeding population of large‐bodied shorebirds. Our estimates of life expectancy for males were comparable to longevity records in godwits (17.3 years ±5.8SE vs. 25–29+ years) and willets (7.7 ± 1.5SE vs. 10+ years). The two species both showed strong breeding site fidelity but differed in rates of mate fidelity. Pairs that reunited and males that switched mates usually nested <300 m from their previous nests, whereas females that switched mates usually moved longer distances >1.1–1.5 km. Returning pairs usually reunited in godwits (85%) but not in willets (28%), possibly because of species differences in adult survival or patterns of migration. Baseline estimates of annual survival for banded‐only birds will be useful for evaluating the potential effects of new tracking tags or the environmental changes that have occurred during the past 20 years. Conservation strategies for large‐bodied shorebirds should be focused on reduction of exposure to anthropogenic mortality because low rates of natural mortality suggest that losses to collisions at breeding sites or harvest at nonbreeding areas are likely to cause additive mortality.
Stereoselective Polymerization of Aromatic Vinyl Polar Monomers
Authors: Li Xin‐Lei , Xu Tie‐Qi 
Published: January 2023

AbstractPolar vinyl polymers, a class of polymers with polar groups as side chains, have significant advantages over conventional nonpolar polyolefin materials in terms of viscosity, toughness, interfacial properties (dyeability and printability), and compatibility with solvents or other polymers. Among them, aromatic polar vinyl polymers are of interest because of their good heat resistance properties. In addition, stereoselective polymerization of aromatic polar vinyl monomers has been rapidly developed because the steric structure of the polymer has a significant impact on its physical properties. In this paper, we review the research progress of stereoselective polymerization catalysts for aromatic polar vinyl monomers in recent years, discuss in detail the influence of ligand structure, electronic effect of substituents, spatial site resistance effect, central rare earth metal species and polymerization solvents on the activity and stereoselectivity of polymerization reactions, and explore the possible mechanism of polymerization reaction.
New Advances in the Synthesis of Emerging Cyclic Amidrazones to Foster Bioisosterism of Azaheterocycles
Authors: Leblanc Johann , Mathé‐Allainmat Monique , Grosse Sandrine , Guillemont Jérôme , Tessier Arnaud , Lebreton Jacques 
Published: January 2023

AbstractThe pace and the scope of new molecules design is often constrained by limitations in synthetic chemistry. The azaheterocyclic amidrazones are of particular interest for bioisosteric considerations in drug discovery. However, the lack of efficient synthetic access has undoubtedly hampered their occurrence in the drug chemical space. Our current results describe a robust synthetic access relying on cyclization of aminohydrazine in presence of various orthoesters by either metal free‐ or metal‐catalyzed condensations. This optimized synthetic access to cyclic amidrazones as original scaffold should inspire the chemist community and further drive innovation in the design of molecular structure for many applications (for example, drugs, materials, dyes).
Best practices to increase your shot at grant funding
Authors: Sutton Halley 
Published: January 2023

LAS VEGAS — “I spent about 16 years at Syracuse University, in their emergency communications department. In [this] my former job, ‘no’ was never an option. I had to find a solution [to whatever problem we faced]. Grant funding is similar,” Shannon Day, Grants Development Consultant, Grants Office, LLC, said.
The effects of shocks to interest rate expectations in the euro area: Estimates at the country level
Authors: Mandler Martin , Scharnagl Michael 
Published: January 2023

AbstractWe estimate the effects of shocks to interest rate expectations on the four largest euro area economies. We identify these shocks in a Bayesian vector autoregressive (BVAR) model augmented by survey expectations. We separate the expectations shocks from standard monetary policy shocks by assuming that they do not affect the current policy rate. Our sign restrictions also ensure that these shocks do not contain central bank information shocks. We find that an expected decline in the future short‐term rate leads to an increase in output and prices. The increases do not become larger for changes to interest rate expectations further in the future, that is, we do not find evidence of a forward guidance puzzle. Using the multicountry structure of our model, we test for cross‐country differences in the shocks' effects and find output and price effects to be greatest in Germany. We also compare the effects of shocks to interest rate expectations to those of standard monetary policy shocks and show the first type to affect output and prices more strongly in Germany and France.
Diminished motor neuron activity driven by abnormal astrocytic EAAT1 glutamate transporter activity in spinal muscular atrophy is not fully restored after lentiviral SMN delivery
Authors: Welby Emily , Ebert Allison D. 
Published: January 2023

AbstractSpinal muscular atrophy (SMA) is characterized by the loss of the lower spinal motor neurons due to survival motor neuron (SMN) deficiency. The motor neuron cell autonomous and non‐cell autonomous disease mechanisms driving early glutamatergic dysfunction, a therapeutically targetable phenotype prior to motor neuron cell loss, remain unclear. Using microelectrode array analysis, we demonstrate that the secretome and cell surface proteins needed for proper synaptic modulation are likely disrupted in human SMA astrocytes and lead to diminished motor neuron activity. While healthy astrocyte conditioned media did not improve SMA motor neuron activity, SMA motor neurons robustly responded to healthy astrocyte neuromodulation in direct contact cultures. This suggests an important role of astrocyte synaptic‐associated plasma membrane proteins and contact‐mediated cellular interactions for proper motor neuron function in SMA. Specifically, we identified a significant reduction of the glutamate Na+ dependent excitatory amino acid transporter EAAT1 within human SMA astrocytes and SMA lumbar spinal cord tissue. The selective inhibition of EAAT1 in healthy co‐cultures phenocopied the diminished neural activity observed in SMA astrocyte co‐cultures. Caveolin‐1, an SMN‐interacting protein previously associated with local translation at the plasma membrane, was abnormally elevated in human SMA astrocytes. Although lentiviral SMN delivery to SMA astrocytes partially rescued EAAT1 expression, limited activity of healthy motor neurons was still observed in SMN‐transduced SMA astrocyte co‐cultures. Together, these data highlight the detrimental impact of astrocyte‐mediated disease mechanisms on motor neuron function in SMA and that SMN delivery may be insufficient to fully restore astrocyte function at the synapse.
Improving bermudagrass in the Southeastern United States with alfalfa as an alternative nitrogen source in grazing systems
Authors: Burt Justin C. , Baxter Lisa L. , Prevatt Christopher G. , Kimberly Mullenix Mary , Stewart R. Lawton , Tucker Jennifer J. 
Published: January 2023

AbstractBackgroundBermudagrass (Cynodon spp.) is one of the most common perennial warm‐season forages grown in the Southeastern United States. The incorporation of alfalfa (Medicago sativa) into bermudagrass pastures could serve as an alternative source of nitrogen (N) while also improving the nutritive value of the forage base.MethodsA 2‐year grazing evaluation arranged in a randomized complete block design was conducted from May to September 2018 (Year 1) and May–August 2019 (Year 2) in Tifton, GA, USA. The objective was to compare the production of alfalfa–bermudagrass mixed pastures (BGA) with bermudagrass monoculture pastures with (BGN) or without (BG) the application of synthetic nitrogen on forage production and stocker cattle performance.ResultsDifferences were observed for average daily gain (p = 0.10), gain per hectare (p = 0.02) and stocking rate (p = 0.01), in that BGA was greatest compared to BGN and BG, which did not differ from each other. Furthermore, BGA had increased forage crude protein (p = 0.01) and total digestible nutrients (p = 0.01) compared to BGN and BG. Finally, BGA provided the greatest economic return per hectare compared to BG or BGN.ConclusionsThese data illustrate improved animal performance and forage nutritive value using alfalfa–bermudagrass systems and offer a viable option for producers seeking alternatives to synthetic N sources for bermudagrass pastures in the Southeastern United States.
Board gender diversity and the cost of equity: What difference does gender quota legislation make?
Authors: Sarang Aitzaz Ahsan Alias , Aubert Nicolas , Hollandts Xavier 
Published: January 2023

AbstractThis study examines the relationship between women directors and the cost of equity (COE). Investigating the French firm's sample, we find a significant negative effect of women directors on the COE. Our results also document that the effect of women directors on reducing the COE is significant for firms that have a critical mass of at least four women directors. Using the difference‐in‐difference (DID) and propensity score matching (PSM) approach, we find that the relationship between female directors and lower equity costs is significant for the period following the Copé–Zimmermann gender quota law. The results show that women directors' presence on corporate boards is also supported by economic reasons. The study provides implications in relation to the Copé–Zimmermann law in France.
Safety and performance of theSpectraOptia apheresis system for white blood cell depletion in patients with elevated white blood cell counts
Authors: Lopert Pamela , Abdelrahman Sohair , Graybill Christopher A. , Rhodes Jack , Dierickx Daan , Schäfer Richard , Reti Marienn , Wu Depei , Hu Yu , Huang Fen , Hu Jianda , He Pengcheng , Liu Ligen , Li Ming , Zhang Xuejun , Bill Jerry 
Published: January 2023

AbstractBackgroundFor the past 30 years, white blood cell depletion (WBCD) or leukocytapheresis has been conducted to rapidly reduce excessive circulating white blood cell (WBC) concentrations in patients at risk for or with symptoms of leukostasis due to hyperleukocytosis. The goal of leukocytapheresis is to prevent or treat acute complications from leukostasis, thereby enabling patients to receive potentially curative chemotherapy.MethodsThis report details the results from a retrospective and a prospective clinical study conducted in the European Union and the People's Republic of China, which assessed the use of the Spectra Optia Apheresis System for leukocytapheresis in patients with hyperleukocytosis. The primary objective of both studies was to the assess the safety and performance of the WBCD procedure in patients with elevated WBC counts.ResultsData were collected from 72 participants completing 87 WBCD procedures. The mean percent change in participant WBC counts post‐procedure was 50.3 ± 21.2% and the collection efficiency (CE1) of the WBCD procedures was 53.7 ± 19.8%. Sixty‐one participants (95.3%) experienced a total of 279 adverse events (AEs) with the majority of the AEs related to post‐procedure changes in laboratory values, which is an anticipated AE in this patient population.ConclusionThe data collected within these studies indicate that the WBCD procedure is safe and well tolerated in patients with hyperleukocytosis as evaluated by percent decrease in WBC count, CE1, and AE incidence.
Effectiveness of denitrification bioreactors with woodchips, corn stover, and phosphate‐sorbing media for simultaneous removal of drainage water N and P in a corn–soybean system
Authors: Oladeji Olawale , Tian Guanglong , Cooke Richard , El‐Naggar Essam , Cox Albert , Zhang Heng , Podczerwinski Edward 
Published: January 2023

AbstractMillions of acres of farmland in the midwestern United States (US) are artificially drained, and this contributes to the export of nitrogen (N) and phosphorus (P) from agricultural land to surface water. Using a 36‐acre tile‐drained farm field, effects of P‐sorbing media in combination with a denitrifying bioreactor system constructed with woodchips (WC) and corn stover (CS) on reducing nutrient export in drainage water were tested for 3 cropping years (2018–2020). The field was divided into three subfields as replicates. In each subfield, the drainage water was divided and separately channeled into three bioreactors, each of which contains one of the three different substrates: WC, CS, and CS–WC (1:1 v/v mixture of CS and WC), randomly assigned. The outlet of each compartment contained a 2.25 L flow‐through chamber filled with activated iron (Fe) filings as P‐sorbing material. Both WC and CS bioreactors were effective in removing drainage NO3− with a 77% (WC), 86% (CS), and 89% (CS–WC) reduction in mean NO3−–N concentration. For the three cropping years, the WC bioreactor reduced the total drainage inorganic N (NO3−–N + NH4+–N) load by 72%, but the CS bioreactor increased the total inorganic N load in the drainage water due to the substantial release of NH4+ with the decomposition of CS. The breakdown of CS also increased drainage P. The NH4+ and P release decreased with the decrease in the proportion of CS; thus, not more than 10% of CS is recommended for blending with WC to enhance the performance of a bioreactor. The P‐sorbing Fe filing media reduced the P loads in drainage by an average of 19% during the 2‐year study.
Funding schemes for infrastructure investment and poverty alleviation in Africa: Evidence from Guinea‐Bissau
Authors: Cateia Júlio Vicente , Vaz Lobo Bittencourt Maurício , Sabadini Carvalho Terciane , Savard Luc 
Published: January 2023

AbstractThis study examines the economic impacts of an infrastructure investment programme in Guinea‐Bissau for the period 2014–2030 using a dynamic computable general equilibrium model. Social accounting matrix (SAM) takes into account informal activities, and the model integrates funding schemes for infrastructure investment. We found that debt‐funded infrastructure investment will generate positive macro‐ and micro‐level externalities in terms of growth and well‐being outcomes across household groups in the urban and rural environments and contribute to inequality reduction. However, direct tax funding scheme is not the best economic development alternative for a country with a low per capita income.
[18F]Fluoropyridine‐losartan: A new approach toward human Positron Emission Tomography imaging of Angiotensin II Type 1 receptors
Authors: Abreu Diaz Aida Mary , Rodriguez Riera Zalua , Lee Yanick , Esteves Luis Miguel , Normandeau Charles‐Olivier , Fezas Baptiste , Hernandez Saiz Alejandro , Tournoux François , Juneau Daniel , DaSilva Jean N. 
Published: January 2023

Angiotensin II type 1 receptors (AT1R) blocker losartan is used in patients with renal and cardiovascular diseases. [18F]fluoropyridine‐losartan has shown favorable binding profile for quantitative renal PET imaging of AT1R with selective binding in rats and pigs, low interference of radiometabolites and appropriate dosimetry for clinical translation. A new approach was developed to produce [18F]fluoropyridine‐losartan in very high molar activity. Automated radiosynthesis was performed in a three‐step, two‐pot, and two‐HPLC‐purification procedure within 2 h. Pure [18F]FPyKYNE was obtained by radiofluorination of NO2PyKYNE and silica‐gel‐HPLC purification (40 ± 9%), preventing the formation of nitropyridine‐losartan in the second step. Conjugation with trityl‐losartan azide via click chemistry, followed by acid hydrolysis, C18‐HPLC purification and reformulation provided [18F]fluoropyridine‐losartan in 11 ± 2% (decay‐corrected from [18F]fluoride, EOB). Using tris[(1‐(3‐hydroxypropyl)‐1H‐1,2,3‐triazol‐4‐yl)methyl]‐amine (THPTA) as a Cu(I)‐stabilizing agent for coupling [18F]FPyKYNE to the unprotected losartan azide afforded [18F]fluoropyridine‐losartan in similar yields (11 ± 3%, decay‐corrected from [18F]fluoride, EOB). Reverse‐phase HPLC was optimized by reducing the pH of the mobile phase to achieve complete purification and high molar activities (467 ± 60 GBq/μmol). The use of radioprotectants prevented tracer radiolysis for 10 h (RCP > 99%). The product passed the quality control testing. This reproducible automated radiosynthesis process will allow in vivo PET imaging of AT1R expression in several diseases.