bluuebunny/crossref_metadata_2025_split huggingface dataset:
Acute care pathway assessed through performance indicators during the COVID-19 pandemic in OECD countries (2020-2021): a scoping review
Authors: Carvalho Ana Sofia V , Broekema Bente , Fernandes Óscar Brito , Klazinga Niek , Kringos Dionne 
Published: March 2023

Abstract
Background: The COVID-19 pandemic severely impacted health systems, leading to care disruptions for non-COVID patients. Performance indicators to continuously monitor acute care, timely reported and internationally accepted, lacked during the COVID-19 pandemic in OECD countries. Methods: Scoping review. Search in Embase and MEDLINE databases. Acute care performance indicators were collected and collated following the care pathway; indicators related to acute general surgery were analyzed separately. Results: A total of 152 studies were included. 2354 indicators regarding general acute care and 301 indicators related to acute general surgery were collected and collated. Indicators focusing on pre-hospital services reported a decreasing trend in the volume of patients: from 225 indicators, 110 (49%) reported a decrease. An increasing trend in pre-hospital treatment times was reported by most of the indicators (n=41;70%) and a decreasing trend in survival rates of out-of-hospital cardiac arrest (n=61;75%). Concerning care provided in the emergency department, most of the indicators (n=752;71%) showed a decreasing trend in admissions, across all levels of urgency (n=26, 51% of highly urgent/level 1 indicators; n=20, 56% of level 2; n=31, 67% of level 3 and 4; and n=43, 78% of non-urgent/level 5 indicators). Indicators assessing the volume of diagnostic and treatment procedures signaled reductions: 65% (n=85) of the indicators and 51% (n=54) of the indicators, respectively. Concerning the mortality rate after admission, most of the indicators (n=23;53%) reported an increasing trend. The subset of indicators assessing acute general surgery showed a decreasing trend in the volume of patients reported by most of the indicators (n=50,49%), stability in clinical severity at admission (n=36,53%), and stability in the volume of surgeries (n=14,47%). Most of the indicators (n=28,65%) reported no change in treatment approach and stable mortality rate (n=11,69%). Conclusions: This review summarizes the performance indicators available in the literature to assess the quality of acute care in OECD countries and their trends during the first year and a half of the COVID-19 pandemic (2020-July2021). These results highlight the relevance of assessing the acute care pathway more regularly and systematically across different clinical entities, to monitor care disruptions and improve the resilience of emergency services to crises.
Recovery of eelgrass Zostera marina following conversion of conventional block and chain moorings to conservation mooring systems in Massachusetts: context dependence, challenges, and management
Authors: Seto Iris , Evans N. Tay , Carr Jillian , Frew Kate , Rousseau Mark , Schenck Forest 
Published: March 2023

Abstract
Eelgrass (Zostera marina) meadows and boat mooring fields co-occur in near-shore, relatively sheltered embayments. Traditional chain moorings create denuded scars in eelgrass meadows due to repeated and chronic scour of the seafloor by the chain, impacting meadow contiguity and quality. This study assessed the recovery of eelgrass into mooring scars following the conversion of traditional chain moorings to floating rode conservation mooring systems (CMS) in three Massachusetts harbors. The magnitude and rate of eelgrass recovery following conversion to floating rode CMS was contingent on the location (e.g., site specific environmental conditions) and the size of the scar associated with the mooring. Eelgrass recovery was inversely correlated with exposure and tidal range, and positively correlated with original scar size. While most scars started to revegetate within two years of mooring conversion, few experienced complete recovery, with a two meter denuded halo persisting around mooring anchors five years post-conversion. In some cases, scar sizes increased where floating rode CMS dragged on the seafloor due to inadequate depth, oversizing of gear, or fouling. Transplanting vegetative eelgrass shoots into scars following conversion to floating rode CMS did not increase the extent or quality of eelgrass habitat restored. We show that floating rode CMS can be an important tool for eelgrass conservation and restoration, however, challenges pertaining to site-specific design, installation, long-term maintenance, and public perception must be addressed to fully realize this potential.
Long-Term Effects of Aflibercept Injections on Lesion-Free Retina in Eyes with Neovascular Age-Related Macular Degeneration
Authors: Demir Nur , Kayhan Belma , Sevincli Sukru , Sonmez Murat 
Published: March 2023

Abstract
Purpose
The study aimed to investigate the adverse effects of long-term aflibercept injections on the lesion-free retina in eyes with neovascular age-related macular degeneration (nAMD).
Methods
This retrospective, comparative study consisted of aflibercept-injected 48 eyes diagnosed with nAMD, treatment-naive 42 nAMD eyes, and 40 eyes with drusen in the control group. The measurements were done with spectral-domain optical coherence tomography. The center of the Early Treatment Diabetic Retinopathy Study (ETDRS) grid was centered at a 3-mm distance from the foveola temporally. Total retinal thickness and the thicknesses of each retinal layer in the outer temporal subfield of the ETDRS grid were measured separately. One-way ANOVA test for normally distributed variables was applied for analyses of differences for the comparison of the results between groups. A p-value of less than 0.05 was considered statistically significant.
Results
The median follow-up time was 51.35 ± 20.92 months. Retinal nerve fiber layer thickness (RNFL) was highest in the treated nAMD group, followed by the treatment-naive nAMD and drusen groups. However, the difference between the treated nAMD and drusen groups showed statistical significance (17.77 ± 2.57 µm; 16.15 ± 1.81 µm, p < 0.05). Total retina, ganglion cell layer, inner plexiform layer, inner nuclear layer, outer plexiform layer, outer nuclear layer, and retina pigment epithelium thicknesses didn’t show a statistically significant difference between the groups (p > 0.05).
Conclusions
The study demonstrated an increase in RNFL thickness in the lesion-free retina after long-term aflibercept treatments. This finding may suggest the expansion of AMD pathology to the normal-appearing retina rather than the aflibercept effect.
Association between fear of COVID-19, religious belief and depressive symptoms in adolescents
Authors: Xiu Meihong , Chen Jianghe , Qu Miao 
Published: March 2023

Abstract
There has been accumulating interest in the association of religion with mental status in adolescents. However, the impact of the interaction between religion and stress on adolescents remains unclear. This study aims to investigate the psychiatric symptoms among religious adolescents at the beginning of the COVID-19 epidemic in China. A total of 11,603 Chinese adolescents were recruited in this survey. The Patient Health Questionnaire-9 (PHQ-9), Generalized Anxiety Disorder-7 Scale (GAD-7), the Connor-Davidson Resilience Scale (CD-RISC), and the Childhood Trauma Questionnaire (CTQ) were assessed for all adolescents. Of 11,069 valid questionnaires collected, 847 (7.7%) reported holding religious beliefs. Religious adolescents showed significantly more severe depressive and anxiety symptoms compared to non-religious adolescents. 249 (2.2%) reported exposure to COVID-19 and had more severe depressive and anxiety symptoms as compared to those without such exposure. Logistic regression analysis revealed that religiosity was a risk factor for the symptoms of depression (OR = 1.37, 95%CI: 1.16–1.61, p < 0.001) and anxiety (OR = 1.49, 95%CI: 1.23–1.79, p < 0.001). Our finding suggests that religious adolescents easily develop depressive and anxiety symptoms, compared to non-religious adolescents. Parents and education workers should regularly monitor adolescents with religious beliefs, especially those who had poor parent-child relationships and emotional or physical abuse.
Exploration of clozapine-induced cardiomyopathy and its mechanism
Authors: Zhang Shangyu , Yang Li , Zen Yujie , Jin Pengyue , Li Yongguo , Tang Renkuan 
Published: March 2023

Abstract

In this study, by pooling the clinical data of patients who died with a history of long-term clozapine use and by examining their hearts, it was found that long-term clozapine use can lead to cardiomyopathy and that its presentation resembles arrhythmogenic right ventricular cardiomyopathy (ARVC) i.e., it exhibits a predominantly right ventricular fatty infiltration with mild left ventricular damage. The cause of clozapine cardiomyopathy was then explored by a transcriptomic approach, which revealed that its clozapine action on cardiomyocytes enriched cardiomyocyte-related differential genes in biological processes such as muscle development and response to hypoxia, as well as pathways such as fatty acid metabolism and cellular autophagy. Combined with the results of pathological examination and transcriptomic analysis, it can be concluded that the long-term action of clozapine on cardiomyocytes leads to cellular autophagy and subsequent structural remodeling of the heart, and in the remodeling affects fatty acid metabolism, which eventually leads to ARVC-like changes.
ZNF500 suppresses the proliferation of breast cancer cells and sensitizes them to chemotherapy by stabilizing p53 in a manner that is competitive to MDM2
Authors: Ma Xiaowen , Fan Mingwei , Yang Kaibo , Wang Yuanyuan , Hu Ran , Guan Mengyao , Hou Yuekang , Ying Jiao , Deng Ning , Li Qingchang , Jiang Guiyang , Zhang Yong , Zhang Xiupeng 
Published: March 2023

Abstract
Background: Zinc finger protein 500 (ZNF500) has an unknown expression pattern and biological function in human tissues. This study was designed to determine what role ZNF500 played in breast cancer progression and chemotherapy resistance.
Methods: Firstly, we assessed the expression pattern of ZNF500 by bioinformatics assay and immunohistochemistry staining. Colony formation assay, MTT assay, EdU assay, flow cytometric analysis, western blotting analysis, mice xenograft model, immunofluorescence assay, RT-PCR, PCR-array, GST-pull down assay and immunoprecipitation assay were performed to investigate the effect of ZNF500 on proliferation, chemotherapy sensitivity and P53-P21-E2F4 signaling axis activation. Secondly, we constructed ZNF500-ΔC2H2, ZNF500-ΔSCAN,ZNF500-ΔKRAB, P53-ΔC, P53-ΔDBD, P53-ΔN mutant plasmids. Immunoprecipitation assay, GST-pull down assay, and western blotting analysis were performed to evaluate interaction among ZNF500, P53 and MDM2, as well as their impact on chemotherapy sensitivity. Finally, immunohistochemistry analysis was used to assess the expression of ZNF500, P53 and E2F4 in clinical tissue samples and its correlation with chemotherapy resistance.
Results:We found that ZNF500 was highly expressed in breast cancer, but negatively correlated with advanced TNM stage, lymph node metastasis and poor prognosis. ZNF500 overexpression abolished in vivo and in vitro breast cancer cell proliferation by activating the p53-p21-E2F4 signaling axis and directly interacted with p53 via its C2H2 domain. This may prevent ubiquitination of p53 in a manner that is competitive to MDM2, thus stabilizing p53. When ZNF500-∆C2H2 was overexpressed, suppressed proliferation of breast cancer cells was neutralized in vitroand in vivo. In human breast cancer tissues, ZNF500 expression was positively correlated with p53and E2F4 expression. ZNF500 expression was significantly lower in patients with Miller/Payne Grade 1–2 than that in those with Miller/PayneGrade 3–5.
Conclusions: Our data suggest that ZNF500 can directly bind to the C-terminal of p53 via its C2H2 domain. This interaction may prevent ubiquitin-mediated degradation by MDM2, thus abrogating the proliferation of breast cancer cells, strengthening DNA damage, and sensitizing breast cancer patients to chemotherapy.
The function of miR-27b on the chemoresistance of hepatocellular carcinoma
Authors: Zhuo Lijuan , Zhan Lingling , Chen Hong , Zhang Wenmin , Gao Meiqin , Huang Aimin 
Published: March 2023

Abstract
The chemoresistance of hepatocellular carcinoma (HCC) is associated with certain microRNA level. Specifically, previous studies have found that miR-27b expression is upregulated in all four HCC drug-resistance cell lines. Despite that, the function of miR-27b in HCC is not clear yet. The purpose of this study was to investigate the effect of miR-27b on chemotherapy resistance in a model of HCC. To establish stable overexpression of miR-27b and negative control HCC cell lines, a lentiviral pre-miR-27b overexpression vector and negative control vector were transfected into each cell line. The expression of miR-27b was detected with quantitative real-time PCR. CCK-8 assay, clone formation assay and immunohistochemical assay were used to detect cell proliferation. Apoptosis and drug sensitivity were detected by flow cytometry and MTT assay, respectively. The level of miR-27b in five HCC cell lines (Huh-7, HepG2, SK-Hep1 and MHCC97h, SMMC7721) was lower than that of in the normal liver cell line LO2. miR-27b level in HCC tissues was also lower than in liver tissues adjacent to the tumor. Two stable miR-27b overexpression HCC cell lines (Huh-7/miR-27b and HepG2/miR-27b) and their control cell lines (Huh-7/NC and HepG2/NC) were successfully constructed. We found that upregulation of miR-27b can reduced chemosensitivity of HCC cell to 5-FU, ADR and CDDP. Simultaneously, miR-27b promote cell apoptosis and suppress cell proliferation. Our study suggests that miR-27b acts as a biomarker to estimate drug sensitivity to chemotherapy in HCC patients. In addition, miR-27b functions as an HCC suppressor.
Classification of Second Order Painlevé Type Equations Under the Mei Symmetrical Transformations
Authors: Rehman Haseeb Ur , Feroze Tooba 
Published: March 2023

Abstract
Ince provided fifty second-order ordinary differential equations of Painlevé type. In this paper, the Mei symmetries correspond to the Lagrangian of the Painlevé-Gambier classification are investigated as well as the Mei invariants along with their respective gauge functions. On the basis of the number of Mei symmetries , these equations are classified. The existence of Mei symmetries can be correlated with the autonomous and non-autonomous properties of ordinary differential equations of Painlevé type.
MSC (2020). 76M60; 22E70; 35A30; 58J70
Pitting Influence on Electrical Capacitance in EHL Rolling Contacts
Authors: Zaiat Anatoly , Ibrahim Karim , Kirchner Eckhard 
Published: March 2023

Abstract
This work presents an investigation on the influence of pitting in EHL rolling contact. The pit-ting geometry comes as an extension on the architecture for multi-physical numerical calculations ofrolling element bearing contacts based on OpenFOAM. The model utilized is built according to thestate-of-the-art for fluid-solid interaction and electro-quasi-static fields. In this framework, the con-tact is reduced to be two dimensional in order to reduce the computing costs needed. The changesin the electric properties, that are caused by pittings, are measured using the electric capacitanceof the contact and put in perspective to the EHL contacts geometry. The investigation deliversan evaluation on how surface degradation, in both the pitting width w and pitting depth d ofthe contact, affects the electric properties. It can be seen that the electrical capacitance changeof different initial geometries has different gradients; both in terms of absolute pitting geometrieslike pitting width w and pitting depth d and relative compared to the reduced radius w/Rr.
A refined weakly-supervised semantic segmentation method on lung adenocarcinoma histopathology images
Authors: Lan Xiaobin , Mei Jiaming , Lin Ruohan , Chen Jiahao , Zhang Yanju 
Published: March 2023

Abstract
Automatic segmentation of tissues from histopathology images is of greatsignificance to reduce the annotation cost hence improve the efficiency of lungadenocarcinoma diagnosis. In the field of weakly supervised semanticsegmentation (WSSS), class activation mapping (CAM) is a widely usedtechnology that only uses image-level label to locate target objects. While themethod based on CAM can locate the target object precisely, it cannot fill theentire object. Therefore, In this paper, we propose a novel method of joint refinederasure attention module (REM) and class re-activation mapping (ReCAM) forimage-level semantic segmentation of histopathology images. Specifically, REMconsists of two components: channel attention and progressive erasure attention.Channel attention dynamically adjusts the weights of each channel based on theirrelevance and progressive erasure attention selectively removes the mostdiscriminative regions to encourage the model to explore more features. Thereason for this is to keep the recognition ability of each feature while expandingthe seed region. Moreover, due to the reason that different tissues may includesimilar features caused by aggressive nature of the malignancy, we employ thereactivation method to enhance the model’s discriminative power. This methodfine-tunes the model parameters by learning an additional fully connected layerwith softmax cross-entropy loss (SCE). We evaluate the proposed model on therecently released LUAD-HistoSeg dataset. The results illustrate that our modelachieved a 76.53% MIoU and outperforms state-of-the-art weakly-supervisedlearning methods.
Theoretical and Experimental Studies on the Influence of Lubricating Film on Dynamic Characteristics of Spindle Bearing
Authors: Yu Jie , Chen Shutong , Yuan Wei , Guo Qianjian , Chi Baotao 
Published: March 2023

Abstract
In this paper, an optimized bearing quasi-static model was established by coupling the influence factors of lubricating oil between ball and raceway, the interaction between cage and ball, and the fluid dynamic pressure distribution pressure of oil film between cage and guide ring with the consideration of the effects of cage and lubricating oil. Compared with Jone’s classical quasi-statics model simulation results and test results, the bearing mechanics model established in this paper is more accurate and feasible. On the basis of such model, the dynamic characteristic parameters of the oil film condition between ball and raceway and the force acting on cage, were analyzed for different working conditions. Results show that the oil film thickness of ball-raceway is more sensitive to the bearing rotating speed greater than the loads. With the increasing of the rotating speed, the normal loads between ball and cage will decrease and the force exerted by distribution pressure of fluid dynamic pressure on cage will increase. However, with the thrust load increasing, they tend to decrease gradually.
Complete degradation of di-n-butyl phthalate by Glutamicibacter sp. strain 0426 with a novel pathway
Authors: Ren Chongyang , Wang Yiying , Wu Yanan , Zhao He-Ping , Li Li 
Published: March 2023

Abstract
Di-n-butyl phthalate (DBP) is widely used as plasticizer that has potential carcinogenic, teratogenic, and endocrine effects. In the present study, an efficient DBP-degrading bacterial strain 0426 was isolated and identified as a Glutamicibacter sp. Strain 0426, which can utilize DBP as the sole source of carbon and energy and completely degraded 300 mg/L of DBP within 12 hours. The optimal conditions (pH 6.9 and 31.7°C) for DBP degradation were determined by response surface methodology and DBP degradation well fitted with the first-order kinetics. Bioaugmentation of contaminated soil with strain 0426 enhanced DBP (1 mg/g soil) degradation, indicating the application potential of strain 0426 for environment DBP removal. Strain 0426 harbors a distinctive DBP hydrolysis mechanism with two parallel benzoate metabolic pathways, which may account for the remarkable performance of DBP degradation. Sequences alignment has shown that an alpha/beta fold hydrolase (WP_083586847.1) contained a conserved catalytic triad and pentapeptide motif (GX1SX2G), of which function is similar to phthalic acid ester (PAEs) hydrolases and lipases that can efficiently catalyze hydrolysis of water-insoluble substrates. Furthermore, phthalic acid was converted to benzoate by decarboxylation, which entered into two different pathways: one is the protocatechuic acid pathway under the role of pca cluster, and the other is the catechol pathway. This study demonstrates a novel DBP degradation pathway, which broadens our understanding of the mechanisms of PAE biodegradation.
An optimized protocol for generating and monitoring conditional orthotopic lung cancer in the KP mouse model at BSL-1
Authors: Deng Haibin , Ge Huixiang , Dubey Christelle , Losmanova Tereza , Medova Michaela , Konstantinidou Georgia , Mutlu Seyran Mathilde , Birrer Fabienne Esther , Brodie Tess Melinda , Stroka Deborah , Wang Wenxiang , Peng Ren-Wang , Dorn Patrick , Marti Thomas Michael 
Published: March 2023

Abstract
Background
The inducible Kras/p53 lung adenocarcinoma mouse model, which faithfully recapitulates human disease, is routinely initiated by the intratracheal instillation of a virus-based Cre recombinase delivery system. Handling virus-based delivery systems requires elevated biosafety levels, e.g., biosafety level 2 (BSL-2). However, in experimental animal research facilities, following exposure to viral vectors in a BSL-2 environment, rodents may not be reclassified to BSL-1 according to standard practice, preventing access to small animal micro-computed tomography (micro-CT) scanners that are typically housed in general access areas such as BSL-1 rooms. Therefore, our goal was to adapt the protocol so that the Cre-induced KP mouse model could be handled under BSL-1 conditions during the entire procedure.
Results
The Kras-Lox-STOP-Lox-G12D/p53 flox/flox (KP)-based lung adenocarcinoma mouse model was activated by intratracheal instillation of either a virus-based Cre delivery system or a gutless, adeno-associated, Cre-expressing vector. Tumor growth was monitored over time by micro-CT. We have successfully substituted the virus-based Cre delivery system with a commercially available, gutless, adeno-associated, Cre-expressing vector that allows the KP mouse model to be handled and imaged in a BSL-1 facility. By optimizing the anesthesia protocol and switching to a microscope-guided vector instillation procedure, productivity was increased and procedure-related complications were significantly reduced. In addition, repeated micro-CT analysis of individual animals allowed us to monitor tumor growth longitudinally, dramatically reducing the number of animals required per experiment.
Conclusion
Modifications to the anesthesia and instillation protocols increased the productivity of the original KP protocol. In addition, the switch to a gutless, adeno-associated, Cre-expressing vector allowed longitudinal monitoring of tumor growth under BSL-1 conditions, significantly reducing the number of animals required for an experiment, in line with the 3R principles.