bluuebunny/crossref_metadata_2025_split huggingface dataset:
Ischemic wound revascularization by the stromal vascular fraction relies on host-donor hybrid vessels
Authors: Vuerich Roman , Groppa Elena , Vodret Simone , Ring Nadja Annelies Ruth , Stocco Chiara , Bossi Fleur , Agostinis Chiara , Cauteruccio Matteo , Colliva Andrea , Ramadan Mohammad , Simoncello Francesca , Benvenuti Federica , Agnelli Anna , Dore Franca , Mazzarol Flavia , Moretti Massimo , Paulitti Alice , Palmisano Silvia , De Manzini Nicolò , Chiesa Mattia , Casaburo Manuel , Raucci Angela , Lorizio Daniela , Pompilio Giulio , Bulla Roberta , Papa Giovanni , Zacchigna Serena 
Published: February 2023

AbstractNonhealing wounds place a significant burden on both quality of life of affected patients and health systems. Skin substitutes are applied to promote the closure of nonhealing wounds, although their efficacy is limited by inadequate vascularization. The stromal vascular fraction (SVF) from the adipose tissue is a promising therapy to overcome this limitation. Despite a few successful clinical trials, its incorporation in the clinical routine has been hampered by their inconsistent results. All these studies concluded by warranting pre-clinical work aimed at both characterizing the cell types composing the SVF and shedding light on their mechanism of action. Here, we established a model of nonhealing wound, in which we applied the SVF in combination with a clinical-grade skin substitute. We purified the SVF cells from transgenic animals to trace their fate after transplantation and observed that it gave rise to a mature vascular network composed of arteries, capillaries, veins, as well as lymphatics, structurally and functionally connected with the host circulation. Then we moved to a human-in-mouse model and confirmed that SVF-derived endothelial cells formed hybrid human-mouse vessels, that were stabilized by perivascular cells. Mechanistically, SVF-derived endothelial cells engrafted and expanded, directly contributing to the formation of new vessels, while a population of fibro-adipogenic progenitors stimulated the expansion of the host vasculature in a paracrine manner. These data have important clinical implications, as they provide a steppingstone toward the reproducible and effective adoption of the SVF as a standard care for nonhealing wounds.
Development of softening and ballasted flocculation as a pretreatment process for seawater desalination through a reverse osmosis membrane
Authors: Yadai Tomohiro , Suzuki Yoshihiro 
Published: February 2023

AbstractEfficient desalination through a reverse osmosis (RO) membrane requires the prior removal of blockade-causing substances from raw seawater. To achieve ultrahigh-speed processing of a pretreatment process for seawater RO desalination, we combine traditional softening with ballasted flocculation (SBF) for Ca2+ and Mg2+ removal. An alkaline mixture of Ca(OH)2 and Na2CO3 was the most suitable softening agent for Ca2+ and Mg2+ removal with a reduced amount of generated sludge. This softening treatment simultaneously removed the suspended solids and bacteria from actual seawater. The settling velocity of the suspended solids generated via seawater softening was extremely low. Under the optimum conditions for desalinating actual seawater using an anionic polymer flocculant and microsand, the settling velocity exceeded 3.5 cm/s, 833 times higher than that of softening without ballasted flocculation. The amount of sludge after standing for 3 min was 76.5% lower in SBF than in conventional softening. The silt density index of the treated seawater met the water-supply standard of RO membranes (i.e., <3.0). Furthermore, the SBF-generated sludge exhibited considerably improved dewatering property than the sludge obtained via conventional softening. SBF can efficiently and quickly remove the causative substances of RO membrane fouling from seawater, thereby improving the treatability of generated sludge. SBF provides a new pretreatment process for seawater desalination using RO membranes.
User-focused evaluation of National Ecological Observatory Network streamflow estimates
Authors: Rhea Spencer , Gubbins Nicholas , DelVecchia Amanda G. , Ross Matthew R. V. , Bernhardt Emily S. 
Published: February 2023

AbstractAccurately estimating stream discharge is crucial for many ecological, biogeochemical, and hydrologic analyses. As of September 2022, The National Ecological Observatory Network (NEON) provided up to 5 years of continuous discharge estimates at 28 streams across the United States. NEON created rating curves at each site in a Bayesian framework, parameterized using hydraulic controls and manual measurements of discharge. Here we evaluate the reliability of these discharge estimates with three approaches. We (1) compared predicted to observed discharge, (2) compared predicted to observed stage, and (3) calculated the proportion of discharge estimates extrapolated beyond field measurements. We considered 1,523 site-months of continuous streamflow predictions published by NEON. Of these, 39% met our highest quality criteria, 11% fell into an intermediate classification, and 50% of site-months were classified as unreliable. We provided diagnostic metrics and categorical evaluations of continuous discharge and stage estimates by month for each site, enabling users to rapidly query for suitable NEON data.
Exposure to infection when accessing groceries reveals racial and socioeconomic inequities in navigating the pandemic
Authors: O’Brien Daniel T. , Ristea Alina , Dass Sarina 
Published: February 2023

AbstractDisasters often create inequitable consequences along racial and socioeconomic lines, but a pandemic is distinctive in that communities must navigate the ongoing hazards of infection exposure. We examine this for accessing essential needs, specifically groceries. We propose three strategies for mitigating risk when accessing groceries: visit grocery stores less often; prioritize generalist grocery stores; seek out stores whose clientele have lower infection rates. The study uses a unique combination of data to examine racial and socioeconomic inequities in the ability to employ these strategies in the census block groups of greater Boston, MA in April 2020, including cellphone-generated GPS records to observe store visits, a resident survey, localized infection rates, and demographic and infrastructural characteristics. We also present an original quantification of the amount of infection risk exposure when visiting grocery stores using visits, volume of visitors at each store, and infection rates of those visitors’ communities. Each of the three strategies for mitigating exposure were employed in Boston, though differentially by community. Communities with more Black and Latinx residents and lower income made relatively more grocery store visits. This was best explained by differential use of grocery delivery services. Exposure and exposure per visit were higher in communities with more Black and Latinx residents and higher infection rates even when accounting for strategies that diminish exposure. The findings highlight two forms of inequities: using wealth to transfer risk to others through grocery deliveries; and behavioral segregation by race that makes it difficult for marginalized communities to avoid hazards.
Logistic regression technique is comparable to complex machine learning algorithms in predicting cognitive impairment related to post intensive care syndrome
Authors: Wu TingTing , Wei YueQing , Wu JingBing , Yi BiLan , Li Hong 
Published: February 2023

AbstractTo evaluate the performance of machine learning (ML) models and to compare it with logistic regression (LR) technique in predicting cognitive impairment related to post intensive care syndrome (PICS-CI). We conducted a prospective observational study of ICU patients at two tertiary hospitals. A cohort of 2079 patients was screened, and finally 481 patients were included. Seven different ML models were considered, decision tree (DT), random forest (RF), XGBoost, neural network (NN), naïve bayes (NB), and support vector machine (SVM), and compared with logistic regression (LR). Discriminative ability was evaluated by area under the receiver operating characteristic curve (AUC), calibration belt plots, and Hosmer–Lemeshow test was used to assess calibration. Decision curve analysis was performed to quantify clinical utility. Duration of delirium, poor Richards–Campbell sleep questionnaire (RCSQ) score, advanced age, and sepsis were the most frequent and important candidates risk factors for PICS-CI. All ML models showed good performance (AUC range: 0.822–0.906). NN model had the highest AUC (0.906 [95% CI 0.857–0.955]), which was slightly higher than, but not significantly different from that of LR (0.898 [95% CI 0.847–0.949]) (P > 0.05, Delong test). Given the overfitting and complexity of some ML models, the LR model was then used to develop a web-based risk calculator to aid decision-making (https://model871010.shinyapps.io/dynnomapp/). In a low dimensional data, LR may yield as good performance as other complex ML models to predict cognitive impairment after ICU hospitalization.
Correlation analysis between immune-related genes and cell infiltration revealed prostate cancer immunotherapy biomarkers linked to T cells gamma delta
Authors: Niu Wenkang , Zhang Tingting , Ma Lei 
Published: February 2023

AbstractProstate cancer (PCa) is a urological malignancy with poor prognosis. Immune-related genes are associated with immune infiltration in prostate cancer, but their role in immunogenic PCa is less well understood. We assessed the infiltration patterns of 22 immune cells in PCa and the relationship of immune-related differentially expressed genes (IDEGs) with them. The 87 IDEGs are involved in the interaction between the extracellular matrix and the tumor microenvironment. The model, including seven IDEGs (SLPI, DES, IAPP, NPY, ISG15, PLA2G2A, and HLA-DMB), showed a good predictive power. The SLPI expression is positively correlated with the infiltration level of T cells gamma delta. In addition, PCa has high infiltration levels in Macrophages M1 (18.07%) and Dendritic cells activated (17.64%). The correlation analysis between IDEGs and immune cell infiltration suggested that PCa immunotherapy biomarkers may be closely related to T cells gamma delta.
Vaccination status and long COVID symptoms in patients discharged from hospital
Authors: Nascimento Teresa Cristina D. C. , do Valle Costa Livia , Ruiz Amanda Danieletto , Ledo Carla B. , Fernandes Valeria Paes Lima , Cardoso Luiz Francisco , Junior José Mauro Vieira , Saretta Roberta , Kalil-Filho Roberto , Drager Luciano F. 
Published: February 2023

AbstractEffective vaccination against coronavirus mitigates the risk of hospitalisation and mortality; however, it is unclear whether vaccination status influences long COVID symptoms in patients who require hospitalisation. The available evidence is limited to outpatients with mild disease. Here, we evaluated 412 patients (age: 60 ± 16 years, 65% males) consecutively admitted to two Hospitals in Brazil due to confirmed coronavirus disease 2019 (COVID-19). Compared with patients with complete vaccination (n = 185) before infection or hospitalisation, those with no or incomplete vaccination (n = 227) were younger and had a lower frequency of several comorbidities. Data during hospitalisation revealed that the no or incomplete vaccination group required more admissions to the intensive care unit (ICU), used more corticosteroids, and had higher rates of pulmonary embolism or deep venous thrombosis than the complete vaccination group. Ninety days after hospital discharge, patients with no or incomplete vaccination presented a higher frequency of symptoms (≥ 1) than patients with complete vaccination (40 vs. 27%; p = 0.013). After adjusting for confounders, no or incomplete vaccination (odds ratio [OR] 1.819; 95% confidence interval [CI] 1.175–2.815), female sex (OR 2.435; 95% CI 1.575–3.764) and ICU admission during hospitalisation (OR 1.697; 95% CI 1.062–2.712) were independently associated with ≥ 1 symptom 90 days after hospital discharge. In conclusion, even in patients with severe COVID-19, vaccination mitigates the probability of long COVID symptoms.
Mechanochemical effects underlying the mechanically activated catalytic hydrogenation of carbon monoxide
Authors: Carta Maria , Sanna Anna Laura , Porcheddu Andrea , Garroni Sebastiano , Delogu Francesco 
Published: February 2023

AbstractIn this work, we highlight and measure the intensity of mechanochemical effects at work in the hydrogenation of carbon monoxide by comparing the activity of a supported Co–Fe catalyst subjected, respectively, to ball milling and simple powder agitation. Paying due regard to the discontinuous nature of ball milling, we show that mechanochemical hydrogenation proceeds at significantly higher rate and disclose its connection with individual impacts. Experimental evidence suggests that the enhanced catalytic activity we observe can be ascribed to local processes affecting the amount of powder that gets involved in individual impacts.
Efficacy evaluation of some fumigants against Fusarium oxysporum and enhancement of tomato growth as elicitor-induced defense responses
Authors: El-Aswad Ahmed F. , Aly Maher I. , Alsahaty Sameh A. , Basyony Ayman B. A. 
Published: February 2023

AbstractFusarium wilt, the most serious soil-borne pathogen, is a serious problem for tomato production worldwide. The presented study evaluated the antifungal activity against Fusarium oxysporum f. sp. lycopersici in vitro and in vivo for nine fumigants. In addition, the research examined the possibility of enhancing the growth of tomato plants in order to increase resistance against this disease by using four chemical inducers. The results indicated that at 20 mg/L, the radial growth of the pathogen was inhibited 100% by formaldehyde and > 80% by phosphine. Among the essential oils investigated, neem oil was the most effective, however, it only achieved 40.54% at 500 mg/L. The values of EC50 for all fumigants, except dimethyl disulfide (DMDS) and carbon disulfide (CS2), were lower than those for thiophanate-methyl. Phosphine was the highest efficient. The elicitors can be arranged based on their effectiveness, gibberellic acid (GA3) > sorbic acid > cytokinin (6-benzylaminopurine) > indole-3-butyric acid. The change in root length, fresh weight, and dry weight was greater with soil drench than with foliar application. The fumigant generators formaldehyde, phosphine and 1,4-dichlorobenzene and bio-fumigants citrus and neem oils as well as elicitors gibberellic and sorbic acid could be one of the promising alternatives to methyl bromide against Fusarium oxysporum as an important component of integrated management of Fusarium wilt.
Metagenome reveals caprine abomasal microbiota diversity at early and late stages of Haemonchus contortus infection
Authors: Aboshady Hadeer M. , Choury Alice , Montout Laura , Félicité Yoann , Godard Xavier , Bambou Jean-Christophe 
Published: February 2023

AbstractHaemonchus contortus is one of the most detrimental gastrointestinal nematode parasites for small ruminants, especially in tropics and subtropics. Gastrointestinal nematode and microbiota share the same microhabitat; thus they interact with each other and their host. Metagenomics tools provide a promising way to examine the alterations in the gastric microbial composition induces by gastrointestinal parasites. In this study, we used metagenomics tools to characterize the impact of H. contortus infection on the caprine abomasal microbiota at early and late stage of infection and compared it with non-infected control. Our results showed that H. contortus infection caused a significant increase in abomasal pH at early (7 days post-infection) and late stage of infection (56 days post-infection). The analysis of alpha and beta diversity showed that the microbiota diversity both in number and in proportion was significantly affected at early and late stage of infection. All microbiota classes are impacted by H. contortus infection but Clostridia and Bacteroidia are more concerned. In infected animals, the genera Prevotella decreased at 7 and 56 days post-infection. Here we showed that the abomasal microbiota was significantly affected early after H. contortus infection, and these changes persist at late stage of the infection.
Quantifying the retention of emotions across story retellings
Authors: He Tianyou , Breithaupt Fritz , Kübler Sandra , Hills Thomas T. 
Published: February 2023

AbstractStory retelling is a fundamental medium for the transmission of information between individuals and among social groups. Besides conveying factual information, stories also contain affective information. Though natural language processing techniques have advanced considerably in recent years, the extent to which machines can be trained to identify and track emotions across retellings is unknown. This study leverages the powerful RoBERTa model, based on a transformer architecture, to derive emotion-rich story embeddings from a unique dataset of 25,728 story retellings. The initial stories were centered around five emotional events (joy, sadness, embarrassment, risk, and disgust—though the stories did not contain these emotion words) and three intensities (high, medium, and low). Our results indicate (1) that RoBERTa can identify emotions in stories it was not trained on, (2) that the five emotions and their intensities are preserved when they are transmitted in the form of retellings, (3) that the emotions in stories are increasingly well-preserved as they experience additional retellings, and (4) that among the five emotions, risk and disgust are least well-preserved, compared with joy, sadness, and embarrassment. This work is a first step toward quantifying situation-driven emotions with machines.
Oleuropein confers neuroprotection against rotenone-induced model of Parkinson’s disease via BDNF/CREB/Akt pathway
Authors: Singh Richa , Zahra Walia , Singh Saumitra Sen , Birla Hareram , Rathore Aaina Singh , Keshri Priyanka Kumari , Dilnashin Hagera , Singh Shekhar , Singh Surya Pratap 
Published: February 2023

AbstractMajor pathological features of Parkinson’s disease (PD) include increase in oxidative stress leading to the aggregation of α-synuclein, mitochondrial dysfunction and apoptosis of dopaminergic neurons. In addition, downregulation of the expression of neurotrophic factors like-Brain Derived Neurotrophic Factor (BDNF) is also involved in PD progression. There has been a lot of interest in trophic factor-based neuroprotective medicines over the past few decades to treat PD symptoms. Rotenone, an insecticide, inhibits the mitochondrial complex I causing overproduction of ROS, oxidative stress, and aggregation of α-synuclein. It has been shown that BDNF and Tropomyosin receptor kinase B (TrkB) interaction initiates the regulation of neuronal cell development and differentiation by the serine/threonine protein kinases like Akt and GSK-3β. Additionally, Transcription factor CREB (cAMP Response Element-binding protein) also determines the gene expression of BDNF. The homeostasis of these signalling cascades is compromised with the progression of PD. Therefore, maintaining the equilibrium of these signalling cascades will delay the onset of PD. Oleuropein (OLE), a polyphenolic compound present in olive leaves has been documented to cross blood brain barrier and shows potent antioxidative property. In the present study, the dose of 8, 16 and 32 mg/kg body weight (bwt) OLE was taken for dose standardisation. The optimised doses of 16 and 32 mg/kg bwt was found to be neuroprotective in Rotenone induced PD mouse model. OLE improves motor impairment and upregulate CREB regulation along with phosphorylation of Akt and GSK-3β in PD mouse. In addition, OLE also reduces the mitochondrial dysfunction by activation of enzyme complexes and downregulates the proapoptotic markers in Rotenone intoxicated mouse model. Overall, our study suggests that OLE may be used as a therapeutic agent for treatment of PD by regulating BDNF/CREB/Akt signalling pathway.
Effects of grain size and small-scale bedform architecture on CO2 saturation from buoyancy-driven flow
Authors: Ni Hailun , Bakhshian Sahar , Meckel T. A. 
Published: February 2023

AbstractSmall-scale (mm-dm scale) heterogeneity has been shown to significantly impact CO2 migration and trapping. To investigate how and why different aspects of small-scale heterogeneity affect the amount of capillary trapping during buoyancy-driven upward migration of CO2, we conducted modified invasion percolation simulations on heterogeneous domains. Realistic simulation domains are constructed by varying two important aspects of small-scale geologic heterogeneity: sedimentary bedform architecture and grain size contrast between the matrix and the laminae facies. Buoyancy-driven flow simulation runs cover 59 bedform architecture and 40 grain size contrast cases. Simulation results show that the domain effective CO2 saturation is strongly affected by both grain size and bedform architecture. At high grain size contrasts, bedforms with continuous ripple lamination at the cm scale tend to retain higher CO2 saturation than bedforms with discontinuous or cross lamination. In addition, the “extremely well sorted” grain sorting cases tend to have lower CO2 saturation than expected for cross-laminated domains. Finally, both a denser CO2 phase and greater interfacial tension increase CO2 saturation. Again, variation in fluid properties seems to have a greater effect on CO2 saturation for cross-laminated domains. This result suggests that differences in bedform architecture can impact how CO2 saturation values respond to other variables such as grain sorting and fluid properties.
Dual blockage of both PD-L1 and CD47 enhances the therapeutic effect of oxaliplatin and FOLFOX in CT-26 mice tumor model
Authors: Alimohammadi Reza , Mahmoodi Chalbatani Ghanbar , Alimohammadi Masoumeh , Ghaffari-Nazari Haniyeh , Rahimi Arezou , Mortaz Esmail , Mossafa Nariman , Boon Louis , Jalali Seyed Amir 
Published: February 2023

AbstractColorectal cancer is a poorly immunogenic. Such property can be reverted by using ICD. However, ICD inducers can also induce the expression of inhibitory checkpoint receptors CD47 and PD-L1 on tumor cells, making CRC tumors resistant to mainly CD8 T cell killing and macrophage-mediated phagocytosis. In this study, we examined the therapeutic effect of Oxaliplatin and FOLFOX regimen in combination with blocking antibodies against CD47 and PD-L1. FOLFOX and Oxaliplatin treatment lead to an increase in CD47 and PD-L1 expression on CT-26 cells invitro and invivo. Combining blocking antibodies against CD47 and PD-L1 with FOLFOX leads to a significant increase in survival and a decrease in tumor size. This triple combining regimen also leads to a significant decrease in Treg and MDSC and a significant increase in CD8 + INF-γ + lymphocytes and M1/M2 macrophage ratio in the tumor microenvironment. Our study showed triple combining therapy with FOLFOX, CD47 and PD-L1 is an effective treatment regimen in CT-26 mice tumor model and may consider as a potential to translate to the clinic.