bluuebunny/crossref_metadata_2025_split huggingface dataset:
Comparison of the Hall Technique and Conventional Compomer Restorations: A 60-Month Follow-up
Authors: ŞEN YAVUZ Betül , KARGUL Betul 
Published: September 2023

Objective: The Hall Technique is one biological strategy for sealing carious lesions with preformed metal crowns in primary molars. This study aimed to compare the Hall Technique's survival rate with conventional compomer restorations in caries management in primary molars for 60 months.
Methods: Children with preformed metal crowns placed with Hall Technique and conventional compomer restorations were invited to Pediatric Dentistry Clinics for a 60-month follow-up. The restorations of these children were evaluated clinically and radiographically. Dental health records of 12 and 24-month follow-up appointments were obtained from the electronic archive. The survival rate of the restorations was evaluated by Kaplan-Meier analysis and the success/failure of the restorations by the Chi-Square test. Restorations with finding such as secondary caries, pulpitis, restoration wear/fracture/loss, crown perforation, inter-radicular radiolucency, and internal root resorption were scored according to major and/or minor failure criteria, while satisfactory ones were scored as successful.
Results: Twenty-six primary molars were included in the study. There was no significant difference in the survival rates of preformed metal crowns placed with the Hall Technique (92.3%) and conventional compomer restorations (84.6%) at 60-month follow-up (2 = 2.455, p = .48). The Hall Technique (84.6%) was found significantly more successful clinically and radiographically compared to conventional compomer restorations (23.1%) according to the success or failure criteria in 60-month follow-up (p < .01).
Conclusion: The Hall Technique was clinically and radiographically more successful than conventional compomer restorations according to the success or failure criteria at 60-month follow-up. The Hall Technique had a similar survival rate to the conventional compomer restorations as well as low failure findings in caries management in primary molars.
Eating Attitudes From a Cardiometabolic Risk Perspective: Psoriasis Sample
Authors: CEYHUN Hacer Akgül , BİLEN Handan 
Published: September 2023

Objective: In this study, we aimed to compare psoriasis patients with healthy controls in terms of impaired eating attitudes and to investigate the relationship of eating attitudes with cardiometabolic and clinical parameters, anxiety, depression, and quality of life.
Methods: 45 psoriasis patients and 45 healthy controls were included in the study. Personal and clinical information form, eating attitude test (EAT-40), body mass index (BMI) and MetS criteria were used for all participants. Psoriasis patients were evaluated with clinical information form, hospital anxiety and depression scale (HADS), dermatological quality of life index (DQLI), psoriasis area and severity index (PASI).
Results: The data of the patient and control groups differed in terms of doing sports, impaired EAT, BMI groups, and metabolic syndrome (MetS). Abnormal eating attitudes such as negative body image, inability to control oneself in eating, overeating, and some restrictive attitudes were significantly higher in the psoriasis group. Overeating, overeating or stress-induced emotional eating, presence of MetS, weight dissatisfaction, frequent dieting to lose weight, some compensatory behaviours, and loss of self-control were significantly higher in patients with BMI>25. EAT points; showed a positive moderate correlation with BMI and HAD-Anxiety. DQLI results; showed a moderate positive correlation with HADAnxiety and PASI scores.
Conclusion: Our study is the first to reveal what kind of disordered eating attitudes are at risk for cardiometabolic diseases in psoriasis patients. In psoriasis patients, the rate of not being able to control their eating behaviour is high. Our results primarily highlight the relationship that can be explained by autonomic reactivity between anxiety and difficulty resisting food cravings. Professional support including psychoeducational, cognitive behavioural therapy, and acceptance-based therapies should be provided to reduce maladaptive reactions and anxiety by improving self-regulation skills.
Dental Age Estimation with Special Emphasis on Age Limits of 12/15 and 18 Years: Detailed Analysis According to Governing Law
Authors: SİNANOĞLU Enver Alper , AKALIN KUNUK Feyza , BAYRAK Seval , ANKARALI Handan 
Published: September 2023

Objective: The aim of this study is to investigate the potential of Demirjian method in estimation of age groups with limits of 12, 15, and 18 years, according to ages of legal responsibilities.
Methods: The panoramic radiographs of the study population aged between 6 to 22 were assessed for third molar (3M) mineralization with Demirjian method with four subgroups. Group 1 consisted of individuals aged between 6 to and 12 years of age, Group 2 were aged between 12 to 15 (Group 2, ≥12,
Workshop On Early Detection Overweight In Adolescents At Karuna Dipa Junior High School In Palu City
Authors: Aslinda Wery , Ansar Ansar , Bahja Bahja 
Published: September 2023

Indonesia as a developing country is one of the contributors to the increasing incidence of Overweight adolescents. The form of activities such as providing counseling and training on early detection of overweight and providing short workshops on making healthy snacks to prevent obesity (Banana Bud) is one of the efforts to prevent obesity in adolescents. The purpose of this community service is to increase adolescent knowledge about overweight prevention, improve adolescent skills in early detection of overweight and in selecting and making snacks to prevent overweight.
This type of community service is providing counseling and training on early detection of overweight as well as providing a short workshop on making healthy snacks to prevent overweight (Banana bud) at Karuna Dipa junior high school in Palu City.
Knowledge assessment results show that an increase in the average knowledge score was 2.9 points (14.5%). For the skill of determining BMI, students achieved the ability of 72% which was assessed from their ability to calculate BMI using discs or using a calculator, while for the determination of overweight, students' abilities reached 83% which was assessed from their ability to use discs to detect overweight.
The conclusion of community service is that there is an increase in knowledge, skills for determining BMI using discs and calculators, and knowledge of making healthy snacks to prevent overweight
Training On Growth Monitoring Of Children In The Work Area Of Kayuwou Health Center
Authors: Kusumawati Dwi Erma , Nurjaya  , Dias Astiza  
Published: September 2023

Growth monitoring is part of the health service activities carried out for infants and toddlers. It aims to improve the quality of early childhood growth and development and the readiness of children to enter formal education which is directed at improving the nutritional, cognitive, mental and psychological health status of children.
The population and sample in this study were 13 Posyandu located in the working area of the Kayuwou Public Health Center, Sindue Tobata District, Donggala Regency. The results show that the correct Posyandu in measuring body weight is only 15.3%, incorrect as much as 84.7%, the measurement of body length and height is only FOUR Posyandu that take measurements of body length and height and in their use fall into the category not exactly. For the correct Posyandu in filling out the KMS and Determining Growth Status, there is only ONE Posyandu (7.7%).
The conclusion of this community service shows an increase in cadres in anthropometric measurements, filling KMS and determining growth status. This community service is important to provide knowledge and improve the skills needed by cadres to be able to determine the nutritional status of toddlers properly and correctly
PIEZO1 is downregulated in glenohumeral chondrocytes in early cuff tear arthropathy following a massive rotator cuff tear in a mouse model
Authors: Anderson Devon E. , Broun Katherine G. , Kundu Paromita , Jing Xingyu , Tang Xiang , Lu Christopher , Kotelsky Alexander , Mannava Sandeep , Lee Whasil 
Published: September 2023

Introduction: A massive rotator cuff tear (RCT) leads to glenohumeral joint destabilization and characteristic degenerative changes, termed cuff tear arthropathy (CTA). Understanding the response of articular cartilage to a massive RCT will elucidate opportunities to promote homeostasis following restoration of joint biomechanics with rotator cuff repair. Mechanically activated calcium-permeating channels, in part, modulate the response of distal femoral chondrocytes in the knee against injurious loading and inflammation. The objective of this study was to investigate PIEZO1-mediated mechanotransduction of glenohumeral articular chondrocytes in the altered biomechanical environment following RCT to ultimately identify potential therapeutic targets to attenuate cartilage degeneration after rotator cuff repair.Methods: First, we quantified mechanical susceptibility of chondrocytes in mouse humeral head cartilage ex vivo with treatments of specific chemical agonists targeting PIEZO1 and TRPV4 channels. Second, using a massive RCT mouse model, chondrocytes were assessed for mechano-vulnerability, PIEZO1 expression, and calcium signaling activity 14-week post-injury, an early stage of CTA.Results: In native humeral head chondrocytes, chemical activation of PIEZO1 (Yoda1) significantly increased chondrocyte mechanical susceptibility against impact loads, while TRPV4 activation (GSK101) significantly decreased impact-induced chondrocyte death. A massive RCT caused morphologic and histologic changes to the glenohumeral joint with decreased sphericity and characteristic bone bruising of the posterior superior quadrant of the humeral head. At early CTA, chondrocytes in RCT limbs exhibit a significantly decreased functional expression of PIEZO1 compared with uninjured or sham controls.Discussion: In contrast to the hypothesis, PIEZO1 expression and activity is not increased, but rather downregulated, after massive RCT at the early stage of cuff tear arthropathy. These results may be secondary to the decreased axial loading after glenohumeral joint decoupling in RCT limbs.
Activity fingerprinting of AMR β-lactamase towards a fast and accurate diagnosis
Authors: Song Chenchen , Sun Xuan , Wang Yao , Bülow Leif , Mecklenburg Michael , Wu Changxin , Meng Qinglai , Xie Bin 
Published: September 2023

Antibiotic resistance has become a serious threat to global public health and economic development. Rapid and accurate identification of a patient status for antimicrobial resistance (AMR) are urgently needed in clinical diagnosis. Here we describe the development of an assay method for activity fingerprinting of AMR β-lactamases using panels of 7 β-lactam antibiotics in 35 min. New Deli Metallo β-lactamase-1 (NDM-1) and penicillinase were demonstrated as two different classes of β-lactamases. The panel consisted of three classes of antibiotics, including: penicillins (penicillin G, piperacillin), cephalosporins (cefepime, ceftriaxone, cefazolin) and carbapenems (meropenem and imipenem). The assay employed a scheme combines the catalytic reaction of AMR β-lactamases on antibiotic substrates with a flow-injected thermometric biosensor that allows the direct detection of the heat generated from the enzymatic catalysis, and eliminates the need for custom substrates and multiple detection schemes. In order to differentiate classes of β-lactamases, characterization of the enzyme activity under different catalytic condition, such as, buffer composition, ion strength and pH were investigated. This assay could provide a tool for fast diagnosis of patient AMR status which makes possible for the future accurate treatment with selected antibiotics.
Clinical benefits of oral anticoagulants in atrial fibrillation patients with dementia: a systematic review and meta-analysis
Authors: Wang Dayang , Xu Xiaoqing , Han Xiaowan , Xie Jing , Zhou Hufang , Peng Wenhua , Pan Guozhong 
Published: September 2023

BackgroundThe management of atrial fibrillation (AF) with oral anticoagulants (OAC) is generally recommended to reduce the risk of stroke. However, the decision to prescribe these medications for patients with AF and dementia remains controversial.MethodsA systematic review and meta-analysis of retrospective cohort studies were conducted. The search encompassed PubMed, Cochrane Library, Web of Science, and Embase databases from inception until May 1st, 2023, with language limited to English. Eligible studies included comparisons between exposure to OAC vs. non-OAC in the AF population with dementia or cognitive impairment. Studies that compared the effects of direct oral anticoagulants (DOAC) and vitamin-K antagonists were also included. The primary outcome was all-cause mortality, and the secondary outcomes were ischemic stroke and major bleeding. This study was registered with PROSPERO (No. CRD42023420678).ResultsA total of five studies (N = 21,962 patients) met the eligibility criteria and were included in this review. The follow-up duration ranged from 1 to 4 years. Meta-analysis demonstrated that OAC treatment was associated with a lower risk of all-cause mortality in AF patients with dementia with a hazard ratio (HR) of 0.79 and a 95% confidence interval (CI) ranging from 0.68 to 0.92, compared to non-OAC treatment. No statistical differences were observed in the risk of major bleeding (HR = 1.12, 95% CI: 0.88–1.42) or ischemic stroke (HR = 0.77, 95% CI: 0.58–1.00). Three studies reported comparisons between DOAC and warfarin; however, pooled analysis was not performed due to heterogeneity.ConclusionThe use of OACs in individuals diagnosed with both AF and dementia holds the potential to reduce all-cause mortality rates, thereby improving the overall clinical prognosis within this specific population.Systematic Review Registrationhttps://www.crd.york.ac.uk/prospero/display_record.php?ID=CRD42023420678, PROSPERO identifier, CRD42023420678.
Mass cytometry reveals the corneal immune cell changes at single cell level in diabetic mice
Authors: Qin Limin , Li Qian , Wang Liqiang , Huang Yifei 
Published: September 2023

IntroductionDiabetic ocular complications include sight-threatening consequences and decreased corneal sensitivity, characterized by decreased tear production, corneal sensitivity and delayed corneal epithelial wound healing. The pathogenesis of diabetic corneal disorders remains largely unknown. Growing evidence implies the participation of immune cells in the development of diabetic corneal diseases. Nonetheless, the immunological changes that result in diabetic corneal problems are largely unknown.MethodsMass cytometry by time of flight (CyTOF) was used to investigate immune cell cluster alterations associated with diabetic corneal disorders. CyTOF test was performed on corneal cells at a single level from 21-week-old diabetic (db/db) and non-diabetic (db/m) mice. A panel of 41 immune-related markers monitored different immune cell types in diabetic corneas. To investigate the proportion of each immune cell subpopulation, an unsupervised clustering method was employed, and T-distributed stochastic neighbor embedding was used to visualize the distinctions between different immune cell subsets.ResultsThrough CyTOF test, we identified 10 immune cell subsets in the corneal tissues. In a novel way, we discovered significant immune alterations in diabetic corneas, including pronounced alterations in T cells and myeloid cell subgroups in diabetic corneas linked to potential biomarkers, including CD103, CCR2, SiglecF, Ly6G, and CD172a. Comprehensive immunological profiling indicated remarkable changes in the immune microenvironment in diabetic corneas, characterized by a notable decrease in CD103+CD8+ tissue-resident memory T (TRM) cells and Tregs, as well as a dramatic increase of γδT cells and subsets of CD11b+Ly6G+ myeloid-derived suppressor cells (MDSCs).ConclusionCyTOF analysis revealed significant alterations in the immune microenvironment during the development of diabetic corneal complications. This study mapped the immune microenvironment landscape of type 2 diabetic corneas, providing a fundamental understanding of immune-driven diabetic corneal disorders.
Phenotypic assay for cytotoxicity assessment of Balamuthia mandrillaris against human neurospheroids
Authors: Whangviboonkij Narisara , Pengsart Worakamol , Chen Zhenzhong , Han Seokgyu , Park Sungsu , Kulkeaw Kasem 
Published: September 2023

IntroductionThe phenotypic screening of drugs against Balamuthia mandrillaris, a neuropathogenic amoeba, involves two simultaneous phases: an initial step to test amoebicidal activity followed by an assay for cytotoxicity to host cells. The emergence of three-dimensional (3D) cell cultures has provided a more physiologically relevant model than traditional 2D cell culture for studying the pathogenicity of B. mandrillaris. However, the measurement of ATP, a critical indicator of cell viability, is complicated by the overgrowth of B. mandrillaris in coculture with host cells during drug screening, making it challenging to differentiate between amoebicidal activity and drug toxicity to human cells.MethodsTo address this limitation, we introduce a novel assay that utilizes three-dimensional hanging spheroid plates (3DHSPs) to evaluate both activities simultaneously on a single platform.Results and discussionOur study showed that the incubation of neurospheroids with clinically isolated B. mandrillaris trophozoites resulted in a loss of neurospheroid integrity, while the ATP levels in the neurospheroids decreased over time, indicating decreased host cell viability. Conversely, ATP levels in isolated trophozoites increased, indicating active parasite metabolism. Our findings suggest that the 3DHSP-based assay can serve as an endpoint for the phenotypic screening of drugs against B. mandrillaris, providing a more efficient and accurate approach for evaluating both parasite cytotoxicity and viability.
Global trends and prospects about synaptic plasticity in Alzheimer’s disease: a bibliometric analysis
Authors: Zhang Yingying , Zhang Junyao , Wang Yinuo , Yao Junyan 
Published: September 2023

Background and purposeIn recent years, synaptic plasticity disorders have been identified as one of the key pathogenic factors and the early pathological characteristics of Alzheimer’s disease (AD). In this study, we tried to use bibliometric analysis to gain a systematic understanding about synaptic plasticity in Alzheimer’s disease.MethodsWe extracted relevant publications from the Web of Science Core Collection (WoSCC) on August 29th, 2022. Then, we used CiteSpace, VOSviewer and other online bibliometric platforms1 to further analyze the obtained data.ResultsA total of 2,348 published articles and reviews about synaptic plasticity in AD from 2002 to 2022 were identified. During the past two decades, the overall trends of the numbers and citations of manuscripts were on the rise. The United States was the leading country with the largest number of publications which showed its crucial role in this field. The collaboration network analysis showed that the United States and China had the most frequent collaboration. In addition, Harvard University was the institution with the greatest number of publications and cited times. Among all authors, Selkoe DJ was the most influential author with the greatest cited times. The journal of Alzheimer’s disease published the maximum number of documents in the field of synaptic plasticity in AD within 20 years. Furthermore, the results of keywords burst detection showed that the hot topics have shifted from the synaptic transmission, precursor protein and plaque formation to neuroinflammation, microglia and alpha synuclein.ConclusionThis study analyzed 2,348 publications with 82,025 references covering the topic of synaptic plasticity in AD and presented the research trends. The results indicated that neuroinflammation, microglia and alpha synuclein were the current research hotspots, which implied the potential clinical applications to AD.
Biomarkers associated with functional improvement after stroke rehabilitation: a systematic review and meta-analysis of randomized controlled trials
Authors: Chen Gengbin , Wu Manfeng , Chen Jialin , Zhang Cailing , Liu Quan , Zhao Yinchun , Xu Guangqing , Lan Yue 
Published: September 2023

ObjectiveThis study aims to identify blood and cerebrospinal fluid biomarkers that are correlated to the functional improvement of stroke patients after rehabilitation therapy, and provide ideas for the treatment and evaluation of stroke patients.MethodsThe PubMed, Web of Science, and Embase databases were searched for articles published in the English language, from inception to December 8, 2022.ResultsA total of 9,810 independent records generated 50 high-quality randomized controlled trials on 119 biomarkers. Among these records, 37 articles were included for the meta-analysis (with a total of 2,567 stroke patients), and 101 peripheral blood and cerebrospinal fluid biomarkers were included for the qualitative analysis. The quantitative analysis results revealed a moderate quality evidence that stroke rehabilitation significantly increased the level of brain-derived neurotrophic factor (BDNF) in serum. Furthermore, the low-quality evidence revealed that stroke rehabilitation significantly increased the concentration of serum noradrenaline (NE), peripheral blood superoxide dismutase (SOD), peripheral blood albumin (ALB), peripheral blood hemoglobin (HB), and peripheral blood catalase (CAT), but significantly decreased the concentration of serum endothelin (ET) and glutamate. In addition, the changes in concentration of these biomarkers were associated with significant improvements in post-stroke function. The serum BNDF suggests that this can be used as a biomarker for non-invasive brain stimulation (NIBS) therapy, and to predict the improvement of stroke patients.ConclusionThe concentration of serum BNDF, NE, ET and glutamate, and peripheral blood SOD, ALB, HB and CAT may suggest the function improvement of stroke patients.