bluuebunny/crossref_metadata_2025_split huggingface dataset:
Evolution Under Dietary Restriction Increases Reproduction at the Cost of Decreased Somatic Growth
Authors: Huang Zhi-Yu , Xi Yi-Long , Wang Qiao , Li Zi-Ai , Shi Bao-Chun , Ge Ya-Li 
Published: April 2023

AbstractDietary restriction (DR) is cited as the most reliable means of extending life span in a wide range of taxa, yet the evolutionary basis of the DR effect on life span remains unclear. The resource reallocation hypothesis proposes that the longevity-extending response of DR is adaptive and stems from the reallocation of resources from reproduction to somatic maintenance under food-limited conditions. However, if DR continues for multiple generations, such a response becomes maladaptive, and genotypes with higher fecundity should be selectively favored over genotypes with longer longevity. To test this prediction, we exposed replicate populations of the rotifer Brachionus dorcas, a model organism for aging and experimental evolution studies, to DR and ad-libitum (AL) diets for 100 days. During the selection experiment, AL-selected populations showed higher growth rates and mictic ratios than DR-selected populations. After approximately 27 asexual generations of selection, populations with a DR selection history had a higher net reproductive rate but lower body volume and ingestion rate in the absence of survival costs than populations with an AL selection history when they were assayed on an AL diet. Our results are inconsistent with the prediction that evolution on sustained DR increases reproduction and reduces life span, and show for the first time that sustained DR selects for clones with higher energy investment in reproduction but lower investment in somatic growth.
Internalization or Dissociation? Negative Age Stereotypes Make You Feel Younger Now But Make You Feel Older Later
Authors: Kornadt Anna E , Weiss David , de Paula Couto Maria Clara , Rothermund Klaus 
Published: April 2023

Abstract

Objectives
Negative age stereotypes have negative, assimilative effects on the subjective aging experience due to internalization processes, but sometimes positive contrast effects are reported as well, reflecting dissociation and downward comparisons. Our aim was thus to compare short-term and long-term consequences of age stereotypes on the subjective aging experience, to test the hypothesis that contrast effects are visible cross-sectionally, whereas internalization processes are observed when considering long-term changes.

Methods
We assessed age stereotypes and subjective age in a core sample of N = 459 participants (initial age range 30–80 years) from the Ageing as Future project across 3 consecutive measurement occasions spanning a longitudinal interval of 10 years. Short-term and long-term effects were estimated with latent growth models by assessing the effects of age stereotypes on the intercepts (cross-sectional) and on the slopes (longitudinal) of subjective age, respectively, while controlling for current self-views.

Results
Age stereotypes had opposite effects on subjective age depending on the time frame. A cross-sectional contrast effect was found, whereas longitudinal effects were assimilative in nature.

Discussion
Our findings support the time-dependent nature of the effects of age stereotypes on the subjective aging experience. Negative age stereotypes temporarily lead to a significantly younger subjective age, indicating dissociation from one’s age group and downward comparison. In the long run, however, negative (positive) age stereotypes become internalized into the self-views of older people and are linked to a relatively older (younger) subjective age.
Community-Engaged Recommendations for Empowering People Living With Dementia to be Research Collaborators
Authors: Griffith Eric E , Monin Joan K , Lepore Michael 
Published: April 2023

Abstract
As cases of Alzheimer’s disease and related dementias (ADRD) increase worldwide, research design has placed additional emphasis on social and behavioral factors that affect ADRD symptomatology and quality of life. Despite this, few studies have incorporated people living with ADRD as research partners. We propose 5 community-engaged recommendations for incorporating people living with ADRD into future research as full collaborators. The proposed recommendations center the experiences of people living with ADRD as crucial contributions to scientific inquiry. The guidelines are based on experiences at a 2-day “Empowering Partnerships” workshop in 2019; post workshop activity continued through 2021 with ongoing collaborations, analysis, and reflective practice. The workshop and subsequent conversations engaged a network of people living with ADRD, informal carepartners, and researchers to collectively build their capacities to partner in all aspects of person-centered research. To empower people living with ADRD as research partners, we recommend that research teams (a) create a flexible schedule of communication and/or meetings to accommodate a wide range of ADRD symptoms, (b) generate team-specific communication strategies/guidelines, (c) incorporate lived experiences of people living with ADRD into research protocols, (d) involve people living with ADRD in all aspects of a project, beginning in the developmental stages, and (e) incorporate skilled facilitators to facilitate communication between stakeholder groups. This multi-vocal approach to research will diversify ADRD research and ensure that projects align with the priorities and capacities of principal stakeholders by incorporating individuals with a wide range of cognitive capabilities that more fully represent the diversity of ADRD experiences.
Estimating the occurrence of slow slip events and earthquakes with an ensemble Kalman filter
Authors: Diab-Montero Hamed Ali , Li Meng , van Dinther Ylona , Vossepoel Femke C 
Published: April 2023

SUMMARYOur ability to forecast earthquakes and slow slip events is hampered by limited information on the current state of stress on faults. Ensemble data assimilation methods permit estimating the state by combining physics-based models and observations, while considering their uncertainties. We use an ensemble Kalman filter (EnKF) to estimate shear stresses, slip rates and the state θ acting on a fault point governed by rate-and-state friction embedded in a 1-D elastic medium. We test the effectiveness of data assimilation by conducting perfect model experiments. We assimilate noised shear-stress and velocity synthetic values acquired at a small distance to the fault. The assimilation of uncertain shear stress observations improves in particular the estimates of shear stress on fault segments hosting slow slip events, while assimilating observations of velocity improves their slip-rate estimation. Both types of observations help equally well to better estimate the state θ. For earthquakes, the shear stress observations improve the estimation of shear stress, slip rates and the state θ, whereas the velocity observations improve in particular the slip-rate estimation. Data assimilation significantly improves the estimates of the temporal occurrence of slow slip events and to a large extent also of earthquakes. Rapid and abrupt changes in velocity and shear stress during earthquakes lead to non-Gaussian priors for subsequent assimilation steps, which breaks the assumption of Gaussian priors of the EnKF. In spite of this, the EnKF still provides estimates that are unexpectedly close to the true evolution. In fact, the forecastability for earthquakes for the same alarm duration is very similar to slow slip events, having a very low miss rate with an alarm duration of just 10 per cent of the recurrence interval of the events. These results confirm that data assimilation is a promising approach for the combination of uncertain physics and indirect, noisy observations for the forecasting of both slow slip events and earthquakes.
Machine learning prediction of groundwater heights from passive seismic wavefield
Authors: Abi Nader A , Albaric J , Steinmann M , Hibert C , Malet J-P , Sue C , Fores B , Marchand A , Gros M , Celle H , Pohl B , Stefani V , Boetsch A 
Published: April 2023

SUMMARY
Most of water reservoirs are underground and therefore challenging to monitor. This is particularly the case of karst aquifers which knowledge is mostly based on sparse spatial and temporal observations. In this study, we propose a new approach, based on a supervised machine learning algorithm, the Random Forests, and continuous seismic noise records, that allows the prediction of the underground river water height. The study site is a karst aquifer in the Jura Mountains (France). An underground river is accessible through an artificial shaft and is instrumented by a hydrological probe. The seismic noise generated by the river is recorded by two broadband seismometers, located underground (20 m depth) and at the surface. The algorithm succeeds in predicting water height thanks to signal energy features. Even weak river-induced noise such as recorded at the surface can be detected and used by the algorithm. Its efficiency, expressed by the Nash–Sutcliffe criterion, is above 95 per cent and 53 per cent for data from the underground and surface seismic stations, respectively.
Laboratory investigation of hydraulic fracturing in granitic rocks using active and passive seismic monitoring
Authors: Butt A , Hedayat A , Moradian O 
Published: April 2023

SUMMARY
Knowledge of the fracturing processes can be important for the optimization of pressurized fluid injection operations in the deep underground rock mass. Active and passive seismic monitoring techniques have been used in the field for tracking or mapping the propagating hydraulic fracture. Although both these monitoring techniques provide valuable information about the generated fracture network, it is difficult for either technique to comprehensibly identify the different processes associated with hydraulic fracturing. The combined active and passive monitoring has the potential for better characterization of the complex hydraulic fracturing phenomena. In this study, laboratory hydraulic fracturing experiments with combined active and passive seismic monitoring were conducted on true triaxially loaded Barre granite cubes with different fluid injection rates. The seismic inelastic fracturing was detected by 16 passive acoustic emission sensors, where 3678 and 2370 seismic source events were detected for the high and low injection rate experiments, respectively. For active monitoring, strong variations in the attributes of signals were observed which were transmitted through four source–receiver pairs, placed both perpendicular and parallel to the generated hydraulic fracture. Positive velocity changes were observed for active sensor pairs with ray paths passing through the generated hydraulic fracture indicating fluid permeation, whereas isolated dry deformation was characterized by a slight but permanent velocity decrease. Compared to velocity, the energy of the active signals was 1–2 orders of magnitude more sensitive to different hydraulic fracturing processes. However, the sensitivity and signatures of the active signal attributes were found to be dependent on the frequency range and direction of ray path with respect to the location of the generated fracture network. Using the coupled evaluation of the active and passive signals we were able to systematically identify various hydraulic fracturing processes including: (1) aseismic deformation, (2) fracture initiation and fluid permeation, (3) pressure build-up, (4) fracture propagation and (5) pressure release and leak-off. The results of this study showed that combining the respective advantages of active and passive seismic techniques and using both of them to monitor the failure processes can facilitate a more comprehensive understanding and better control of the hydraulic stimulations in subsurface operations.
An appraisal using magnetic data of the continent-to-ocean transition structure west of Iberia
Authors: Neres Marta , Ranero César R 
Published: April 2023

SUMMARYAbout half of the rifted margins purportedly formed by extension with minor magmatism. The conceptual models of those magma-poor systems are greatly influenced by the continent-to-ocean transition structure of the archetypal magma-poor West Iberia Margin. In the past, interpretation of magnetic data of West Iberia has been used to constrain conceptual rifting models, including the structure of the transition from the exhumed mantle domain to the oceanic crust formed at a spreading centre. However, uncertainties on geophysical data were generally not considered leading to overdetailed interpretations. We use synthetic magnetic modelling to show that magnetic data acquired at sea level cannot resolve subhorizontal lithological layering in deep-water continental margins. We then present new magnetic modelling guided by a refined velocity model of the wide-angle seismic IAM-9 profile that shows that the magnetic J-anomaly correlates with oceanic crust that abuts exhumed mantle across a vertical boundary. This well-constrained observation supports that seafloor spreading initiated abruptly, terminating mantle exhumation. Conventional wisdom dictates that the sudden efficient melt extraction relates to a mechanical threshold during lithospheric thinning and concomitant asthenospheric upwelling under which melt can migrate toward the surface. However, our results support that mantle melting creating oceanic crust was probably not driven by gradual lithospheric thinning and asthenospheric upwelling, but by seafloor spreading centre propagation that cut across the lithosphere, creating the abrupt structure.
Preoperative Use of Multiple Advanced Therapies Is Not Associated With Endoscopic Inflammatory Pouch Diseases
Authors: Powers Joseph Carter , Cohen Benjamin L , Rieder Florian , Click Benjamin H , Lyu Ruishen , Westbrook Katherine , Hull Tracy , Holubar Stefan , Regueiro Miguel D , Qazi Taha 
Published: April 2023

Abstract

Background
Patients with an ileal pouch-anal anastomosis (IPAA) can experience pouch inflammation postoperatively. The use of antitumor necrosis factor (anti-TNF) biologics may be associated with pouch inflammation, but limited data exist on the impact of multiple advanced therapies on development of subsequent pouch inflammation. The aim of this study was to assess for an association between preoperative use of multiple advanced therapies and risk of endoscopically detected inflammatory pouch diseases (EIPDs).

Methods
We performed a retrospective analysis of ulcerative colitis (UC) and indeterminate colitis (IBDU) patients who underwent an IPAA at a quaternary care center from January 2015 to December 2019. Patients were grouped based on number and type of preoperative drug exposures. The primary outcome was EIPD within 5 years of IPAA.

Results
Two hundred ninety-eight patients were included in this analysis. Most of these patients had UC (95.0%) and demonstrated pancolonic disease distribution (86.1%). The majority of patients were male (57.4%) and underwent surgery for medically refractory disease (79.2%). The overall median age at surgery was 38.6 years. Preoperatively, 68 patients were biologic/small molecule-naïve, 125 received anti-TNF agents only, and 105 received non-anti-TNF agents only or multiple classes. Ninety-one patients developed EIPD. There was no significant association between type (P = .38) or number (P = .58) of exposures and EIPD, but older individuals had a lower risk of EIPD (P = .001; hazard ratio, 0.972; 95% confidence interval, 0.956-0.989).

Conclusion
Development of EIPD was not associated with number or type of preoperative advanced therapies.
Beyond Dimorphism: Body Size Variation Among Adult Orangutans Is Not Dichotomous by Sex
Authors: Kralick Alexandra E , O'Connell Caitlin A , Bastian Meredith L , Hoke Morgan K , Zemel Babette S , Schurr Theodore G , Tocheri Matthew W 
Published: April 2023

SynopsisAmong extant great apes, orangutans are considered the most sexually dimorphic in body size. However, the expression of sexual dimorphism in orangutans is more complex than simply males being larger than females. At sexual maturity, some male orangutans develop cheek pads (flanges), while other males remain unflanged even after becoming reproductively capable. Sometimes flange development is delayed in otherwise sexually mature males for a few years. In other cases, flange development is delayed for many years or decades, with some males even spending their entire lifespan as unflanged adults. Thus, unflanged males of various chronological ages can be mistakenly identified as “subadults.” Unflanged adult males are typically described as “female-sized,” but this may simply reflect the fact that unflanged male body size has only ever been measured in peri-pubescent individuals. In this study, we measured the skeletons of 111 wild adult orangutans (Pongo spp.), including 20 unflanged males, 45 flanged males, and 46 females, resulting in the largest skeletal sample of unflanged males yet studied. We assessed long bone lengths (as a proxy for stature) for all 111 individuals and recorded weights-at-death, femoral head diameters, bi-iliac breadths, and long bone cross-sectional areas (CSA) (as proxies for mass) for 27 of these individuals, including seven flanged males, three adult confirmed-unflanged males, and three young adult likely-unflanged males. ANOVA and Kruskal–Wallis tests with Tukey and Dunn post-hoc pairwise comparisons, respectively, showed that body sizes for young adult unflanged males are similar to those of the adult females in the sample (all P ≥ 0.09 except bi-iliac breadth), whereas body sizes for adult unflanged males ranged between those of adult flanged males and adult females for several measurements (all P &lt; 0.001). Thus, sexually mature male orangutans exhibit body sizes that range from the female end of the spectrum to the flanged male end of the spectrum. These results exemplify that the term “sexual dimorphism” fails to capture the full range of variation in adult orangutan body size. By including adult unflanged males in analyses of body size and other aspects of morphology, not as aberrations but as an expected part of orangutan variation, we may begin to shift the way that we think about features typically considered dichotomous according to biological sex.
Identification and engineering efflux transporters for improved L-homoserine production in Escherichia coli
Authors: Ding Chao , Zhang Jiwei , Qiao Jinfang , Ma Zhenping , Liu Pi , Liu Jun , Liu Qingdai , Xu Ning 
Published: April 2023

Abstract

Aims
This study aimed to functionally identify the potential L-homoserine transporters in Escherichia coli, and to generate the promising beneficial mutants by targeted directed evolution for improving the robustness and efficiency of microbial cell factories.

Methods and results
By constructing a series of gene deletion and overexpression strains, L-homoserine tolerance assays revealed that RhtA was an efficient and major L-homoserine exporter in E. coli, whereas RhtB and RhtC exhibited relatively weak transport activities for L-homoserine. Real-time RT-PCR analysis suggested that the expression levels of these three target mRNAs were generally variably enhanced when cells were subjected to L-homoserine stress. Based on in vivo continuous directed evolution and growth-couple selections, three beneficial mutations of RhtA exporter (A22V, P119L, and T235I) with clearly increased tolerance against L-homoserine stress were quickly obtained after two rounds of mutagenesis-selection cycles. L-homoserine export assay revealed that the RhtA mutants exhibited different degrees of improvement in L-homoserine export capacity. Further studies suggested that a combination of these beneficial sites led to synergistic effects on conferring L-homoserine-resistance phenotypes. Moreover, the introduction of RhtA beneficial mutants into the L-homoserine-producing strains could facilitate increased amounts of L-homoserine in the shake-flask fermentation.

Conclusions
In this study, we provided further evidence that RhtA serves as a major L-homoserine exporter in E. coli, and obtained several RhtA beneficial mutants, including A22V, P119L, and T235I that contributed to improving the L-homoserine resistance phenotypes and the production efficiency in microbial chassis.
Sheep with ovarian androgen excess have fibrosis and follicular arrest with increased mRNA abundance for steroidogenic enzymes and gonadotropin receptors
Authors: Abedal-Majed Mohamed A , Abuajamieh Mohannad , Al-Qaisi Mohmmad , Sargent Kevin M , Titi Hosam H , Alnimer Mufeed A , Abdelqader Anas , Shamoun Ahmad I , Cupp Andrea S 
Published: April 2023

Abstract
An androgen excess ovarian micro-environment may limit follicle progression in sheep. Two populations of ewes with divergent follicular fluid androstenedione (A4) were identified in a flock in Jordan: High A4; (A4) ≥ 30 ng/mL, (N = 12) or Control A4 (Control); A4 ≤ 15 ng/mL; (N = 12). We hypothesized High A4 ewes would have increased steroidogenic enzyme mRNA abundance, inflammation, and follicular arrest. Messenger RNA abundance for steroidogenic enzymes StAR, CYP17A1, CYP11A1, and HSD3B1 were increased in theca cells while CYP17A1, CYP19A1, and HSD3B1 were increased in granulosa cells in High A4 ewes compared to Control. Gonadotropin receptor mRNA expression for LHCGR was increased in theca and FSHR in granulosa in High A4 ewes. Messenger RNA expression of FOS when reduced, increases expression of CYP17A1 which was observed in High A4 granulosa cells compared to Control. Furthermore, High A4 ewes had greater numbers of primordial follicles (P &lt; 0.001) and fewer developing follicles compared to Control before, and after 7 d of culture, indicating follicular arrest was not alleviated by cortex culture. Increased fibrosis in the ovarian cortex was detected in High A4 ewes relative to Control (P &lt; 0.001) suggesting increased inflammation and altered extracellular matrix deposition. Thus, this High A4 ewes population has similar characteristics to High A4 cows and women with polycystic ovary syndrome suggesting that naturally occurring androgen excess occurs in multiple species and may be a causative factor in follicular arrest and subsequent female sub- or infertility.
Implementing the National Dense Breast Reporting Standard, Expanding Supplemental Screening Using Current Guidelines, and the Proposed Find It Early Act
Authors: Berg Wendie A , Seitzman Robin L , Pushkin JoAnn 
Published: April 2023

Abstract
Thirty-eight states and the District of Columbia (DC) have dense breast notification laws that mandate varying levels of patient notification about breast density after a mammogram, and these cover over 90% of American women. On March 10, 2023, the Food and Drug Administration issued a final rule amending regulations under the Mammography Quality Standards Act for a national dense breast reporting standard for both patient results letters and mammogram reports. Effective September 10, 2024, letters will be required to tell a woman her breasts are “dense” or “not dense,” that dense tissue makes it harder to find cancers on a mammogram, and that it increases the risk of developing cancer. Women with dense breasts will also be told that other imaging tests in addition to a mammogram may help find cancers. The specific density category can be added (eg, if mandated by a state “inform” law). Reports to providers must include the Breast Imaging Reporting and Data System density category. Implementing appropriate supplemental screening should be based on patient risk for missed breast cancer on mammography; such assessment should include consideration of breast density and other risk factors. This article discusses strategies for implementation. Currently 21 states and DC have varying insurance laws for supplemental breast imaging; in addition, Oklahoma requires coverage for diagnostic breast imaging. A federal insurance bill, the Find It Early Act, has been introduced that would ensure no-cost screening and diagnostic imaging for women with dense breasts or at increased risk and close loopholes in state laws.