bluuebunny/crossref_metadata_2025_split huggingface dataset:
Probing the dynamic landscape of peptides in membrane mimics by
synergized NMR experiments and MD simulations
Authors: Ollila Samuli , Nencini Ricky , Regnier Morgan , Backlund Sofia , Mantzari Efstathia , Dunn Cory 
Published: September 2023

Abstract
Dynamic interactions between peptides and lipid membranes are crucial in many biological processes and biomedical applications. Monitoring peptide dynamics in a membrane environment has been limited by the lack of experimental methods that could directly detect the fast (picosecond to nanosecond) timescale dynamics of peptides in this environment. Spin relaxation times from NMR experiments are sensitive to such motions, but their applications are often limited by complications in sample preparation and interpretation of the data. Here we show that the detailed dynamic landscape of peptide-membrane mimics can be determined by a synergistic combination of solution state NMR experiments and molecular dynamics (MD) simulations. Solution state NMR experiments are straightforward to implement without an excessive amount of sample, while direct combination of spin relaxation data to MD simulations enables detailed interpretation of dynamic landscapes of both peptide and membrane mimics. The interpretation of NMR data from transmembrane, peripheral, and tail anchored peptides indicate that peptides and detergent molecules do not rotate together as a rigid body, but rotate in a viscous medium composed of detergent micelle. Spin relaxation times also provide indirect information on peptide conformational ensembles. This work gives new perspectives on peptide conformational ensembles and dynamics in membrane environments.
Survival Benefit of Surgical Resection for Primary Lung Cancer with Liver Metastases: A Propensity Score-Matched Analysis
Authors: Fan Yongfei , Li Xinwei , Tong Jichun , Lou Ming 
Published: September 2023

Abstract
Purpose
Surgery is the main modality of comprehensive tumor treatment, and there are fewer studies on surgery in lung cancer patients with liver metastases.
Methods
In this study, clinical data on lung cancer patients with liver metastases were obtained from the Surveillance, Epidemiology, and End Results (SEER) database. Patients treated with cancer-directed surgery (CDS) by Propensity Score Matching (PSM) are matched 1:1 to patients not treated with CDS. The impact of CDS treatment and clinical factors on patient prognosis was explored Kaplan-Meier survival analysis, univariate and multivariate Cox analysis, and Cox risk proportional regression models.
Results
A total of 6,632 lung cancer patients with liver metastases were included in this study, including 83 patients treated with CDS (1.25%) and 6549 patients not treated with CDS (98.75%). By PSM matching, 83 patients not treated with CDS were obtained. Survival analysis showed that the median survival of patients in the CDS group was 9 months (95% confidence interval (CI):7–15 months) longer than in the No-CDS group at 3 months (95% CI:2–6 months). Cox risk proportional regression models indicated that No-CDS, male and N2-N3 staging were prognostic risk factors, whereas chemotherapy were prognostic protective factors. In addition, univariate and multivariate Cox analyses showed that No-CDS and men were independent prognostic risk factors, whereas chemotherapy were independent prognostic protective factors.
Conclusion
Our findings suggest that CDS treatment can improve the prognosis of patients with lung cancer with liver metastases, which will provide a reference for further randomized controlled clinical trials.
Spectrum-dependent image convolutional processing via a 2D polarization-sensitive photodetector
Authors: Wei Zhongming , Yu Yali , Xiong Tao , Zhou Ziqi , Liu Duanyang , Liu Yue-Yang , Yang Juehan 
Published: September 2023

Abstract
With the advancement of photodetectors and the widespread deployment of high-definition image sensors, the growth in processing capacity of traditional processors significantly lags behind the demands of real-time image processing. Consequently, achieving real-time image processing at the sensor level has emerged as a prominent research domain in the photodetector field, holding potential applications in various fields including remote sensing, autonomous driving, and road monitoring. This advancement underscores the need for photodetectors with enhanced multifunctional integration capabilities, capable of performing real-time computations on optical or electrical signals. In this study, we employ an innovative two-dimensional p-type semiconductor GaTe0.5Se0.5 as the light absorption layer to construct a photodetector with a polarization-sensitive wide-spectral photoresponse within the wavelength range of 255 to 1064 nm. This design enables the realization of a photodetector that integrates real-time sensing and computation. Leveraging the wide-spectral photoresponse of the photodetector, wide-spectral imaging was realized within the wavelength range of 390–810 nm during experimentation. Furthermore, by utilizing the tunable photoresponses of the photodetector through varying polarization angles, real-time image convolutional processing is enabled, aided by configuring appropriate convolution kernels based on the polarization-sensitive photoresponse values. Our proof-of-concept device, featuring spectrum-dependent polarization-sensitive photoresponses across a wide-spectral range, successfully accomplishes real-time image convolutional processing, including sharpness and edge extraction operations, thereby facilitating more precise capture of image details. The innovative design of the polarization-sensitive wide-spectral GaTe0.5Se0.5-based photodetector constitutes a notable contribution to the domain of real-time image perception and processing.
Trends in adverse drug reactions between 2011 and 2022 in a tertiary hospital in China through retrospective analysis
Authors: Liu Yao , Yang Liu , Li Teng , Yang Lisha , Li Yaling , wu zhigui 
Published: September 2023

Abstract
Adverse drug reactions (ADRs) are unavoidable phenomena in the process of medication administration and can easily cause harm to patients. In order to understand the characteristics and patterns of ADRs, this article analyses the ADR reported by a tertiary hospital in China from 2011 and 2022. We retrospectively analyzed the trends of ADRs over these 10 years, including the distribution of ADRs, basic patient profiles, evaluation of the association of ADRs, route of administration, classification of drugs, and organs/systems involved in the ADRs. From 2011 to 2022, a total of 7,367 ADRs were reported. Over this 10-year period, the proportion of infants has been decreasing, and the rate of the juvenile group and the senior citizen has been increasing. The highest number of ADRs occurred via intravenous infusion. Among the organs/systems involved in adverse drug reactions, the skin and its accessories were mostly damaged, but the rate of ADRs in blood system increased significantly. Antibacterial drugs were the first cause of adverse drug reactions, followed by anti-tumor drugs. Through the changes related to ADRs in the past 10 years, we need to pay more attention to the adverse drug reactions caused by drugs, and further strengthen the monitoring of ADRs to reduce the occurrence of ADRs, and decrease the damage caused by adverse drug reactions, to ensure the life and health of patients.
Fitting a square beam in a square camera: Novel condenser apertures for low-dose transmission electron microscopy
Authors: Brown Hamish , Smith Dan , Wardle Benjamin , Hanssen Eric 
Published: September 2023

Abstract
In transmission electron microscopy (TEM), cameras are square or rectangular but beams are round so the circular lobes irradiate adjacent areas, precluding further neighboring acquisition for beam sensitive samples. We present condenser aperture plates with square and rectangular shapes which improve the efficiency of area usage by 70%. We demonstrate their compatibility with high-resolution cryogenic TEM by reconstructing a 1.8 Å map of equine apo-ferritin.
Identifying high-spin Fe3+(S=5/2) as active center for acidic oxygen reduction
Authors: Sun Shi-Gang , Zhao Kuang-Min , Wang Yucheng , Su Yaqiong , Li Guang , Huang Huan , Shi Hai-Yan , Zhou Zhi-You 
Published: September 2023

Abstract
The active spin state of a single Fe catalytic center in acidic oxygen reduction reaction (ORR) remains uncertain, posing a significant challenge for the precise design of Fe-based ORR catalysts. To address it, we synthesized a conjugate-bridged dual-metal iron phthalocyanine molecule that exhibits an identical Fe site and high catalytic activity comparable to real catalysts. This molecular model addresses the limitations of current model catalysts that fail to meet simultaneously both structural and activity requirements. Compared to the non-conjugate-bridged molecule, the conjugate-bridged dual-metal iron phthalocyanine molecule displayed a 76-fold increase in turnover frequency. By combining spin characterization techniques, in-situ electrochemical infrared spectroscopy, and theoretical calculations, we proposed that the conjugate bridging of two FeN4 sites induces a high-spin Fe3+(S=5/2) state. This state allows for optimal adsorption strength of hydroxyl groups, neither too strong nor too weak, resulting in high catalytic activity that approaches the peak performance. The conclusion of Fe3+(S=5/2) state as the active site was further confirmed by a linear correlation between the content of Fe3+(S=5/2) state and catalytic activity. This study deepens our comprehension of active sites and contributes to the precise design of Fe-based ORR catalysts and beyond.
Marvelous Machine-Learning-Based Breast Cancer Predictive Model
Authors: s.Anthoniraj  , S.Saravanakumar  , ANANDRAJ M , ANJAIAH P 
Published: September 2023

Abstract
Breast cancer is a major health concern for women all over the world. In order to reduce mortality rates and provide the most effective treatment, Histopathology image prognosis is essential. When a pathologist examines a biopsy specimen under a microscope, they are engaging in histopathology. The pathologist looks for the picture, determines its type, labels it, and assigns a grade. Tissue architecture, cell distribution, and cellular form all play a role in determining whether a histopathological scan is benign or malignant. Manual picture classification is the slowest and most error-prone method. Automated diagnosis based on machine learning is necessary for early and precise diagnosis, but this challenge has prevented it from being addressed thus far. In this study, we apply curvelet transform to a picture that has been segmented using k-means clustering to isolate individual cell nuclei. We analysed data from the Wisconsin Diagnosis Breast Cancer database for this article. in the context of similar studies in the literature. It is demonstrated that compared to another machine learning algorithm, the IICA-ANN IICA-KNN and IICA-SVM-KNN method using the logistic algorithm achieves 98.04% accuracy.
Laparoscopic two-stage Fowler-Stephens orchiopexy via Hesselbach’s triangle for the intra-abdominal testes in pediatric patients
Authors: Fu ShuQi , Li Ji 
Published: September 2023

Abstract
Purpose To validate the feasibility and advantages of laparoscopic two-stage Fowler-Stephens (F-S) orchiopexy for managing intra-abdominal testes via Hesselbach's triangle in pediatric patients.Materials and Methods Retrospective analysis was conducted on pediatric patients with impalpable cryptorchidism between June 2017 and September 2022. All cases underwent laparoscopic exploration, and those with intra-abdominal testes were included in this study. Laparoscopic Fowler-Stephens staged operation was performed on all eligible cases. The testicular gubernaculum and vas deferens blood supply during the first stage, as well as collateral circulation of vas deferens blood supply during the second stage were observed. Postoperative color ultrasound was utilized to monitor testicular volume and parenchymal echo.Results A total of 41 cases with intra-abdominal testes were included in the study. Results from the first stage of surgery indicated a positive correlation between higher testicular position and increased dysplasia of the gubernaculum. During the second stage, all vas deferens exhibited thicker blood supply compared to preoperative measurements. All testes were successfully descended into the middle scrotum without re-ascent during postoperative follow-up, and no instances of testicular atrophy or abnormal parenchymal echo were observed.Conclusions The testicular gubernaculum is less developed with the higher testicular position. Staged surgery is helpful to establish adequate collateral circulation of the vas deferens blood supply. Laparoscopic Fowler-Stephens orchiopexy through Hesselbach’s triangle approach is safe and effective,which can lower the testicle to a satisfactory position.
Detection of pit and fissure sealant microleakage using autofluorescence
Authors: Nam Sang-Mi , Ku Hye-min , Lee Eun-song , Kim Baek Il 
Published: September 2023

Abstract
Objectives
The aim of this study was to evaluate the feasibility of detecting the presence and severity of microleakage of pit and fissure sealant using Quantitative Light-induced Fluorescence (QLF) technology.
Methods
The areas of interest (AOI) were a total of 160 pit and fissure sites obtained from the occlusal surfaces of 40 permanent teeth. Fluorescent images were acquired using a QLF device, and the ΔFmax of each AOI was analysed. After staining and cross-sectioning of the teeth, histological dye penetration was scored on a 0–3 scale. The relationship between ΔFmax and microleakage depth was analysed, and the areas under the curve (AUC) were calculated.
Results
ΔFmax showed a significant increase as microleakage depth increased, and a strong correlation with histological scores (r = -0.72, P < 0.001). ΔFmax increased 2.6 times between a sound sealant margin and severe dye penetration. AUC analysis showed a high diagnostic accuracy of microleakage depth (AUC = 0.83–0.91). The AUC that differentiated the outer half microleakage of the sealant (histological score 0 vs 1–3) was the highest at 0.91.
Conclusions
The use of autofluorescence detection based on QLF technology enabled the detection of microleakages in sealants non-destructively and demonstrated excellent diagnostic validity.
Blast Furnace process optimization for sustainable Iron making
Authors: Bhagyaraj D 
Published: September 2023

Abstract
A stable and efficient operation of the ironmaking blast furnace is prerequisite to reduce the greenhouse gas emission. JSW Steel, Salem have been proposed many projects to reduce greenhouse gas emission, for example Higher O2 enrichment, H2 gas injection, Waste plastic injection, Natural gas and use of biomass reductant. Additionally, the operation stability and efficiency have a large influence on gas utilization and the rate of reductants and emissions, which are all important and mutually interdependent factors. This study leveraged advanced statistical tools, mathematical models, and technical research to assess the blast furnace process and detect anomalies. Subsequent to this analysis, a series of optimization measures were implemented, driven by the study's insights. These actions targeted blast furnace process enhancement, leading to heightened production rates, reduced fuel consumption, and diminished greenhouse gas emissions. This work epitomizes the commitment to harmonizing iron production efficiency with environmental sustainability.
Towards Stronger Illumination Robustness of Local Feature Detection and Description based on Auxiliary Learning
Authors: Bian Houqin , Fan Shihe , Zhang Haolin , Qin Lunming , Cui Haoyang , Wang Xi 
Published: September 2023

Abstract

Local feature detection and description play a crucial role in various computer vision tasks, including image matching. Variations in illumination conditions significantly affect the accuracy of these applications. However, existing methods inadequately address this issue. In this paper, a novel algorithm based on Illumination Auxiliary Learning Module (IALM) is introduced. Firstly, a new local feature extractor named Illumination Auxiliary SuperPoint (IA-SuperPoint) is established, based on the integration of IALM and SuperPoint. Secondly, illumination-aware auxiliary training focuses on capturing the effects of illumination variations during feature extraction through tailored loss functions and jointly learning mechanisms. Lastly, in order to evaluate the illumination robustness of local features, a metric is proposed by simulating various illumination disturbances. Experiments on HPatches and RDNIM datasets demonstrate that the performance of local feature extraction is greatly improved by our method. Especially for matching local features with different illumination conditions, our method gains at most 16\% improvement on the state-of-the-art methods.
The Impacts of Protein Supplementation and Semen Exposure on Uterine Cytokines in Beef Heifers
Authors: McLean Kyle J. , Brandt Kiernan J. , Ault-Seay Taylor B. , Payton Rebecca R. , Schneider Liesel G. , Edwards J. Lannett , Myer Phillip R. , Rhinehart Justin D. 
Published: September 2023

Abstract
Reproductive efficiency is largely impacted during heifer development which generally requires nutrient supplementation for proper maturation. Nutritional status can also influence inflammation within the reproductive tract. Therefore, we hypothesized that cytokine concentrations in uterine luminal fluid (ULF) will be impacted by protein supplementation following artificial insemination (AI). Commercial heifers (n = 60) were utilized to determine the effects of protein supplementation and AI on cytokine concentrations in ULF. Heifers were randomly assigned to one of three supplemental treatments: 1) CON (10% crude protein [CP]), 2) P20 (20% CP), and 3) P40 (40% CP). All heifers underwent estrus synchronization and ULF was collected 14 d after insemination. Cytokine profiles were constructed in MetaboAnalyst 5.0 and R Studio was used for individual cytokine analyses. Control heifers had increased (P = 0.05) MIP-1β concentrations (148.7 ± 123.9pg) over P20 heifers (42.3 ± 123.9pg) and P40 heifers (75.5 ± 123.9pg) were intermediate. Semen exposure (1877 ± 550pg) tended (P = 0.06) to increase concentrations of IP-10 compared with heifers who were not inseminated (1556 ± 550pg). In conclusion, protein supplementation and exposure to semen had a limited impact on the cytokine profiles and individual cytokine concentrations in the ULF of heifers following AI but did identify three cytokines providing potential targets important in early gestation.
Identification of stem cell-related gene signature and integrative analyses in patients with lower-grade gliomas
Authors: Jiang Longyang , Zhu Jun , Yang Xuping , Zhang Chengbin , Wei Qiming , Chen Kun , Li Junxin , Wang Shurong 
Published: September 2023

Abstract
Background: Cancer stem cell genes were proven to be associated with the prognosis of multiple cancers. Nevertheless, the prognosis and role of stem cell division-related genes in lower-grade glioma (LGG) remains unclear.
Objective: This study constructs a reliable risk model of stem cell division-related genes for predicting the prognosis of LGG patients.
Methods: The mutation data, FPKM data and corresponding clinical information of 511 LGG patients were downloaded from the TCGA database. We carried out LASSO regression analysis and multivariate cox regression analysis to build a risk gene model. The Kaplan-Meier survival analysis was used to evaluate the overall survival (OS) rate of LGG patients. The ROC curve assesses its power across the entire data set. The risk score model was validated in the Chinese Glioma Genome Atlas (CGGA). We employed univariate independent prognostic analysis and multifactor independent prognostic analysis to determine whether risk scores could be used as independent prognostic factors to predict the clinicopathological characteristics of patients with LGG. A nomogram was constructed to predict the prognosis of LGG patients. In addition, we analyzed the relationship between high and low risk groups and immune infiltration and drug sensitivity.
Results: 11 genes were selected (EV12B, ING2, MLLT3, NCOA3, NOTCH1, PRDM15, SFRP2, SMYD5, THOC2, TIAL1 and WNT7A) from TCGA to construct a risk model, which was validated in CGGA. A nomogram accompanied by calibration curves was presented for predicting 1-, 3-, and 5-year survival in LGG patients. LGG patients in the low-risk group had a longer survival than those in the high-risk group. The 11 stem cell division-related genes might serveas reliable diagnostic biomarkers in LGG. In addition, the risk score was related to immune infiltration and drug sensitivity. Drug sensitivity analysis showed Bortrezomib (p<0.0001), Etoposide (p<0.0001), Tamoxifen (p<0.0001), and Temozolomide (p<0.0001) were more sensitive in the high-risk group than in the low-risk group.
Conclusion: Our study built a prognostic model consisting of 11 stem cell division-related genes for predicting the prognosis of LGG patients. This may be a new biomarker for predicting LGG. These preliminary findings may provide some useful values for clinical prognosis of LGG and future studies.
Genetic characterization, structural and evolutionary adaptations of Small Heat shock proteins involved in mitigating environmental stress in Fishes
Authors: Sultana Mehwish , Parveen Shakeela , Tayyab Muhammad , Hussain Muhammad 
Published: September 2023

Abstract
In the current investigation, a total of 42 full-length, non-redundant small heat shock proteins (sHsps) were detected in Cyprinus carpio, Labeo rohita, Danio rerio, Salmo salar, Oncorhynchus mykiss, and Clupea harengus. The sHsps genes were classified into three groups based on phylogenetic analysis. All the sHsps were shown to have higher aliphatic index values, which is an indication that these proteins are more thermally stable. The hydrophilic nature of sHsps was deduced from the fact that all fish species had negative GRAVY scores. In all of the representative fish species, sHsps genes were assigned to distinct chromosomes in an inconsistent and unequal manner. Segmental duplications are the main events that have contributed to the expansion of the sHsps genes in all species. We were also able to determine the selective pressure that was placed on particular codons, and we discovered several significant coding sites within the coding region of sHsps. Eventually, diversifying positive selection was found to be connected with evolutionary changes in sHsps proteins, which shows that gene evolution controlled the fish adaption event in response to environmental conditions. Clarification of the links between sHsps and environmental stress in fish will be achieved through rigorous genomic comparison, which will also yield substantial new insights.