bluuebunny/crossref_metadata_2025_split huggingface dataset:
The preliminary study on the microwave ablation of multifocal papillary thyroid microcarcinoma
Authors: zhou hui-di , Yu Xin-yu , Wei Ying , zhao zhenlong , Peng Li-Li , Li Yan , Lu Nai-cong , Yu Ming-an 
Published: March 2023

Abstract

Purpose
To evaluate the feasibility, efficiency, and safety of MWA for PTMC.
Methods
This was a retrospective study, the date of patients who underwent MWA for multifocal PTMC from October 2016 to December 2021 were reviewed. After ablation, the changes in tumor size and volume, as well as the rate of technical success, tumor disappearance, disease progression, and complications were assessed. According to the tumor location, the cases were further divided into unilateral multifocal disease (UMD) subgroup and bilateral multifocal disease (BMD) subgroup. And further analyses were carried out.
Results
There was a total of 94 cases enrolled in the present study, which included 24 males and 70 females. The median age was 40 years (22–66 years); the median follow-up time was 14 months (6–48 months). Complete ablation was achieved in all enrolled cases. Therefore, the technical success rate was 100%. Due to expanding ablation, the MD and volume of the ablation zone increased at the 1st and 3rd months after ablation and decreased from 12th month after ablation (p < 0.05 for all). The total complete tumor disappearance rate was (45/94, 47.87%), including, 40.625% (13/32) in the UMD subgroup vs. 51.61% (32/62) in the BMD subgroup (p = 0.312). The total disease progression rate was 4 (4/94, 4.26%), including 6.25% (2/32) in the UMD subgroup vs. 3.23% (2/62) in the BMD subgroup (p = 0.881). The overall complication rate was 4.26% (4/94).
Conclusion
This preliminary study indicates that MWA is a safe and effective treatment for multifocal PTMC.
Mediating role of obesity on the association between disadvantaged neighborhoods and intracortical myelination
Authors: Kilpatrick Lisa , Zhang Keying , Dong Tien , Gee Gilbert , Beltran-Sanchez Hiram , Wang May , Labus Jennifer , Naliboff Bruce , Mayer Emeran , Gupta Arpana 
Published: March 2023

Abstract
We investigated the relationship between neighborhood disadvantage (area deprivation index [ADI]) and intracortical myelination (T1-weighted/T2-weighted ratio at deep to superficial cortical levels), and the potential mediating role of the body mass index (BMI) and perceived stress in 92 adults. Worse ADI was correlated with increased BMI and perceived stress (p's<.05). Non-rotated partial least squares analysis revealed associations between worse ADI and decreased myelination in middle/deep cortex in supramarginal, temporal, and primary motor regions and increased myelination in superficial cortex in medial prefrontal and cingulate regions (p<.001); thus, neighborhood disadvantage may influence the flexibility of information processing involved in reward, emotion regulation, and cognition. Structural equation modelling revealed increased BMI as partially mediating the relationship between worse ADI and observed myelination increases (p=.02). Further, trans-fatty acid intake was correlated with observed myelination increases (p=.03), suggesting the importance of dietary quality. These data further suggest ramifications of neighborhood disadvantage on brain health.
High-Speed Flexible Near-Infrared Organic Photodiode Beyond Silicon for Optical Communication
Authors: Zhu Yu , Chen Hongbin , Han Ruiman , Qin Hao , Hu Lianghao , Yao Zhaoyang , Liu Hang , Ma Yanfeng , Wan Xiangjian , Li Guanghui , Chen Yongsheng 
Published: March 2023

Abstract
Optical communication is a particularly compelling technology to tackle the speed and capacity bottlenecks in data communication in modern society. Currently, silicon photodetector plays a dominant role in the high-speed optical communication across the visible-near infrared spectrum. However, its intrinsic rigid structure, high working bias, and low responsivity largely limit its applications in the next-generation flexible optoelectronic devices. Herein, we report a solution-processed flexible organic photodetector (OPD) based on a narrow-bandgap nonfullerene acceptors, which exhibits a remarkable response time of 91 ns and peaking responsivity of 0.53 A W-1 (λ = 830 nm) at zero bias, exceeding the values obtained in the state-of-the-art high-speed commercial Si photodetector (326 ns; 0.26 A W-1). This exceptional performance benefits from the low parasitic capacitance and high charge mobility due to low trap states and energetic disorder in the organic photoactive film. More significantly, the flexible OPD exhibits negligible performance attenuation (<1%) after bending for 500 cycles, and maintain 96% of its initial performance even after 550 h of indoor exposure. Furthermore, it also demonstrates a high data transmission rate of 80 MHz with a with a bit-error-rate of 3.5×10-4, offering great potential in next-generation high-speed flexible optical communication system.
Landslide evolution assessment based on sequential InSAR methods in the Kunming transmission line corridor
Authors: Xie Chuhang , Zheng Zezhong , Zhou Fangrong , Ma Yi , Wen Gang 
Published: March 2023

Abstract
The security of the transmission line corridor is an important guarantee for the sustainable supply of electricity and an important prerequisite for the rapid development of the economy. Transmission corridors located in high mountains and valleys are often threatened by geological disasters, which seriously affect their stable operation. The main idea of this research is to explore a landslide in the Kunming transmission corridor in November 2021 and analyze its reason. In this study, we investigated the landslide in Kunming using 79 Sentinel-1A Synthetic Aperture Radar (SAR) images acquired from July 2020 to October 2021 (40 ascending images and 39 descending images) with sequential Interferometric Synthetic Aperture Radar (InSAR) methods. Then, the deformation changes before the landslide were analyzed in many ways. Influencing factors (i.e. precipitation, formation lithology, and vegetation coverage) indicated a high correlation between landslide locations and those of the study region. Seasonal variations in deformation were related to precipitation. The landslide in the Kunming transmission line corridor was mainly attributed to precipitation, carbonate karstification, and vegetation coverage. Finally, the correlation between deformation changes before the landslide and influencing factors in the Kunming transmission line corridor were determined.
Exploring influential factors of CO2 emissions in China’s cities using machine learning techniques
Authors: xiang kun , Yu Haofei , Du Hao , Hasan Md Hasibul , Wei Siyi , Xiang Xiangyun 
Published: March 2023

Abstract
Investigating the factors that exert an influence on CO2 emissions represents a critical undertaking for the formulation of effective policies aimed at reducing such emissions. Numerous past studies have attempted to explore the potential relationships between CO2 emissions and a variety of potential influencing factors. However, many of these investigations have been constrained by the inability to fit large sample datasets, as well as by the limitations of conventional research methods in addressing non-linear relationships. The aforementioned insufficiencies have resulted in a situation wherein a restricted set of factors can be examined in tandem within a singular model. This restriction has impeded the comprehensive investigation of multiple variables and their potential interrelationships. To bridge these research gaps, this research employed machine learning models to fit voluminous datasets concerning urban CO2 emissions and socioeconomic factors. This research also incorporated explainable techniques to disentangle the intricate relationships between the factors under investigation. The results demonstrated that urban fiscal structure, urban energy consumption, urban land use, and urban capital accumulation represented the primary factors driving urban CO2 emissions. The ALE test was employed to discern the specific relationships between these factors and CO2 emissions, ultimately revealing that almost all of these factors positively impacted CO2 emissions as their values rise. In both univariate and bivariate ALE tests, Expenditures on Science and Technology had emerged as a highly influential feature in terms of its impact on urban CO2 emissions. While the interaction effects elicited by Expenditures on Science and Technology had the potential to curb urban CO2 emissions in several ways, this research identified that these effects may be constrained. The relationship identified between urban socio-economic development and CO2 emissions also indicated that achieving low-carbon development in urban areas necessitates optimal resource allocation and the transformation of energy consumption structures. Furthermore, each city needs to tailor its low-carbon development pathway to its distinct characteristics.
Relationship between serum interleukin-6 levels and severity of coronary artery disease undergoing percutaneous coronary intervention
Authors: Bouzidi Nadia , Gamra Habib 
Published: March 2023

Abstract
Background: Cytokines play a potential role in atherosclerosis pathogenesis and progression. We investigated the association of interleukin-6 (IL-6) with the angiographic severity of coronary artery disease (CAD).
Methods: 310 angiografically diagnosed CAD patients and 210 controls were enrolled in this study. CAD patients were stratified according to IL-6 cut-off value into high levels IL-6 group (≥ 9.5 pg/mL) and low levels IL-6 group (< 9.5 pg/mL). The severity of CAD was assessed according to Gensini score (GS), artery stenosis degree and the number of vessels involved. The mean age was 60.3 ± 11.0 years.
Results:The level of IL-6 in patients was increased compared to controls and ranged from 1.5 to 3640.0 pg/mL. High levels of IL-6 were significantly associated with high levels of GS (>40) but not with stenosis degree and vessel score. GS levels were significantly more elevated in patients with high levels of IL-6 group than in low IL6 levels patients (60.6 ± 39.5 vs 46.7 ± 37.2; p = 0.027). The analysis of the ROC curve performed in myocardial infarction patients showed that IL-6 (AUC: 0.941 (CI 95% 0.886, 0.997; p<0.001) could be a powerful predictor marker in evaluating the infarct size after myocardial infarction when compared to myonecrosis biomarkers.
Conclusions: IL-6 levels were associated with the severity of CAD assessed by the GS. Based on the highest levels of IL-6 measured in patients with STEMI, our study strongly suggests that IL-6 could be a powerful marker in evaluating the myocardial necrosis.
ClinicalTrials.gov Number: NCT03075566 (09/03/2017)
Prevention of horizontal transfer of laboratory plasmids to environmental bacteria: comparison of the effectiveness of a few disinfection approaches to degrade DNA.
Authors: LORET Suzanne Marie , HABIB Boutaina , ROMAIN Pierre , ROBA Agnès , REBOUL Angéline 
Published: March 2023

Abstract
The routine work of any molecular biology laboratory includes the daily use of microorganisms, including strains of E. coli, transformed with a variety of plasmids expressing at least one antibiotic resistance gene (ARG). To verify the effectiveness of disinfection methods on laboratory liquid waste, bacteria isolated from laboratory and research institute drains were identified by 16S ribosomal RNA sequencing and tested for the presence of an origin of replication and several ARGs frequently found in laboratory plasmids. Surprisingly, the origin of replication of Enterobacteriaceae plasmids was detected in strains of non-Enterobacteriaceae bacteria suggesting that interspecific transfer of laboratory plasmids had occurred. Using quantitative Polymerase Chain Reaction, we determined the Decimal reduction value (D-value, expressed as concentration of disinfectant or length of physical treatment) of several decontamination methods for their DNA degradation effect on cultures of E. coli Top10 transformed with a kanamycin resistant plasmid (pET28A + or pEGFP-C2). The estimated D-values were 0,7 M for Sulfuric, 6,3% for a commercial P3 disinfectant, 25 minutes for steam sterilization at 121°C and 49 minutes for disinfection by UVC. A 20-minute treatment of bacteria cultures with a final concentration of 1–10% sodium hypochlorite was found to be ineffective in completely destroying a bacteria plasmid gene marker (coding for the pBR322 origin of replication). Residual DNA from HClO treated cells was 60%, while it decreased under 10% using the commercial disinfectant P3 diluted at 5%. As the degradation was uncomplete in both cases, to prevent the horizontal transfer of laboratory ARGs to environmental bacteria, disinfected liquid waste should not be released in sewage without additional plasmid destruction treatment.
Exploring the effects of short-course antibiotics on children's gut flora by using 16S rRNA gene sequencing: a case-control study
Authors: Zhou Yuhan , Chen Xianglian , Wang Tongtong , Huang Riyan 
Published: March 2023

Abstract

Background
With the widespread use of antibiotics, more attention has been paid to their side effects. We paid extra attention to the impact of antibiotics on children's bodies. Therefore, we analyzed the characteristic changes in the gut flora of children after antibiotic treatment to explore the pathogenesis of antibiotic-associated diseases in more depth and to provide a basis for diagnosis and treatment.
Methods
We recruited 28 children with respiratory tract infections in the western district of Zhuhai, China, and divided them into three treatment groups based on antibiotic type. We took stool samples from children before and 3-5 days after antibiotic treatment. 16S rRNA gene sequencing was used to analyze the effects of antibiotic therapy on the gut flora of children. Continuous nonparametric data are represented as median values and analyzed using the Wilcoxon rank-sum test.
Results
While alpha diversity analysis found no significant changes in the mean abundance of the gut flora of children after a short course of antibiotic treatment, beta diversity analysis demonstrated significant changes in the composition and diversity of the gut flora of children even after a short course of antibiotic therapy. We also found that meloxicillin sulbactam can inhibit the growth of Proteobacteria, Bacteroidetes, and Verrucomicrobia, ceftriaxone inhibits Verrucomicrobia and Bacteroides, and azithromycin inhibits Fusobacteria, Actinobacteria, Proteobacteria, and Verrucomicrobia. We further performed a comparative analysis at the genus level and found significantly different clusters in each group. Finally, we found that azithromycin had the greatest effect on the metabolic function of intestinal flora, followed by ceftriaxone, and no significant change in the metabolic process of intestinal flora after meloxicillin sulbactam treatment.
Conclusions
Antibiotic treatment significantly affects the diversity of intestinal flora in children, even after a short course of antibiotic treatment. Different classes of antibiotics affect diverse flora primarily, leading to varying alterations in metabolic function. Meanwhile, we identified a series of intestinal flora that differed significantly after antibiotic treatment. These groups of flora could be used as biomarkers to provide an additional basis for diagnosing and treating antibiotic-associated diseases.
Chondroitin sulfate proteoglycan 4 provides new treatment approach to prevent peritoneal dissemination in ovarian cancer
Authors: Uno Kaname , Koya Yoshihiro , Yoshihara Masato , Iyoshi Shohei , Kitami Kazuhisa , Sugiyama Mai , Miyamoto Emiri , Mogi Kazumasa , Fujimoto Hiroki , Yamakita Yoshihiko , Wang Xinhui , Ferrone Soldano , Nawa Akihiro , Kajiyama Hiroaki 
Published: March 2023

Abstract
BACKGROUND: Most epithelial ovarian cancer (EOC) patients are diagnosed with peritoneal dissemination. Cellular interactions are an important aspect to induce peritoneal disseminations. Our study aimed to reveal the role of chondroitin sulfate proteoglycan 4 (CSPG4) in EOC with a major focus on cell-cell interactions.
METHODS: We examined the expression of CSPG4 in clinical samples. The proliferation, migration, and invasion abilities of the CSPG4-knockdown cells were assessed. We also assessed the role of CSPG4 in spheroid formation and peritoneal metastasis in vivo model using sh-CSPG4 EOC cell lines.
RESULTS: Of clinical samples, 23 (44.2%) samples were expressed CSPG4. CSPG4 was associated with a worse prognosis in EOC patients. When CSPG4 was knockdown, the cell proliferation, migration, and invasion abilities were significantly decreased, and spheroid formation was significantly inhibited compared to control cells. Proteomic analyses showed changes in the expression of proteins related to cell movement pathways. The number of peritoneal disseminations and EOC spheroids in ascites were significantly decreased in sh-CSPG4 mouse models. Reduced CSPG4 expression was observed in lymphoid enhancer-binding factor1-inhibited cell.
CONCLUSION: CSPG4 is associated with aggressive features of EOC and poor prognosis. CSPG4 could be a new treatment target to block peritoneal metastasis.
Single-cell CRISPR screening characterizes transcriptional deregulation in T-cell acute lymphoblastic leukemia
Authors: Meyers Sarah , Gielen Olga , Cools Jan , Demeyer Sofie 
Published: March 2023

Abstract
T-cell acute lymphoblastic leukemia (T-ALL) is an aggressive type of leukemia caused by accumulation of genetic alterations in T-cell progenitors. However, for many genes it remains unknown how loss-of-function mutations contribute to disease development. Single-cell CRISPR screening in ex vivo cultured primary murine pro-T cells allowed us to study the transcriptomic impact of such alterations and to link this with effects on cell proliferation and survival. We first targeted 17 well-studied T-cell regulators and defined key transcriptional signatures, such as NOTCH, MYC, STAT and E2F. A second screen targeted 42 poorly characterized genes and identified gene clusters with E2F/MYC and STAT/NOTCH signatures having opposing roles. These analyses identified a cluster of genes, including multiple chromatin modifiers, regulating Myc expression. Additionally, Spi1 was identified as an essential gene for cell survival, associated with the MYC regulon. Bcl11b inactivation conferred the strongest growth advantage and was associated with JAK/STAT upregulation, corresponding with publicly available patient data. Bcl11b inactivation together with mutant JAK3 transformed pro-T cells to cytokine-independent growth. With this data, we characterized tumor suppressors and oncogenes in T-ALL, providing insight in the mechanisms of leukemia development.
Maternal auts2a contribution is a major intergenerational driver of neurodevelopment and behavior
Authors: Clément Antoine , Merdrignac Constance , Brionne Aurélien , Nguyen Thaovi , Montfort Jerome , Guyomar Cervin , Dauvé Alexandra , Herpin Amaury , Colson Violaine , Bobe Julien 
Published: March 2023

Abstract
AUTS2 gene has been associated with numerous human pathologies including autism. The respective importance of its maternal and paternal contribution remains however unknown. Here we show using a vertebrate model that the lack of maternal, but not paternal, gene contribution triggers reduced embryonic survival and long-term macroscopic and behavioral differences, including reduced head size and reduced environment recognition abilities and anxiety. Maternal auts2 modulates maternally-inherited factors that control, in turn, early embryonic gene expression through transcriptional and post-transcriptional regulations, and ultimately regulate numerous transcription factors and evolutionarily conserved signaling pathways during neurodevelopment. We observed a significant enrichment in genes associated with human neuropathologies among genes that are under maternal auts2control. We report that AUTS2 belongs to a group of 45 evolutionarily-conserved maternally-expressed genes associated with behavior and neurodevelopment in vertebrates and linked to a diversity of human diseases. These results shed new light on intergenerational determinism of neurodevelopment and behavior in vertebrates, including in the context of human pathologies associated with AUTS2.
Epidemiological differences between the sexes in adolescent patients with lumbar spondylolysis in Japan
Authors: Asai Reo , Tatsumura Masaki , Gamada Hisanori , Okuwaki Shun , Eto Fumihiko , Nagashima Katsuya , Takeuchi Yousuke , Funayama Toru , Mammoto Takeo , Hirano Atsushi , Yamazaki Masashi 
Published: March 2023

Abstract
Background: Lumbar spondylolysis, a common identifiable cause of low back pain in young athletes, reportedly has a higher incidence rate in males. However, the reason for its higher incidence in males is not clear. This study aimed to investigate the epidemiological differences between the sexes in adolescent patients with lumbar spondylolysis.
Methods: A retrospective study was conducted in 197 males and 64 females diagnosed with lumbar spondylolysis. These patients visited our hospital from April 2014 to March 2020 with their main complaint being low back pain, and they were followed-up until the end of their treatment. We investigated associations between lumbar spondylosis, their background factors, and characteristics of the lesions and analyzed their treatment results.
Results: Males had a higher prevalence of spina bifida occulta (SBO) (p=0.0026), more lesions with bone marrow edema (p=0.0097), and more lesions in the L5 vertebrae (p=0.021) than females. The age at diagnosis, bone union rate, and treatment period were not significantly different between the sexes.
Conclusion: The higher prevalence of SBO in males may explain the sex differences in the incidence of lumbar spondylolysis. The progression of lumbar spondylolysis is however, more advanced in females, thus making a proper early diagnosis, especially in females, important.
Aggressive surgical approach with vascular resection and reconstruction for retroperitoneal sarcomas: a systematic review
Authors: Hu Hankui , Guo Qiang , Zhao Jichun , Huang Bin , Du Xiaojiong 
Published: March 2023

Abstract
Background and aim

Surgery is the mainstay of treatment and completeness of surgical resection is critical to achieve local control for retroperitoneal sarcoma (RPS). En-bloc resection of adjacent organs, including major abdominal vessels, is often required to achieve negative margins. The aim of this review was to summarise the available evidence to assess the relative benefits and disadvantages of an aggressive surgical approach with vascular resection in patients with retroperitoneal sarcoma (RPS).

Methods

We searched PubMed, the Cochrane Library, and EMBASE for relevant studies published from inception up to August 1, 2022. We performed a systematic review of the available studies to assess the safety and long-term survival results of vascular resection for RPS.

Results

We identified a total of 23 studies for our review. Overall postoperative in-hospital or 30-day mortality rate of patients with primary iliocaval leiomyosarcoma was 3% (11/359), and the major complication rate was 13%. The recurrence-free survival (RFS) rates after the follow-up period varied between 15% and 52%, and the 5-year overall survival (OS) rates ranged from 25–78%. Overall postoperative in-hospital or 30-day mortality rate of patients with RPSs receiving vascular resection was 3%, and the major complication rate was 27%. The RFS rates after the follow-up period were 18–86%, and the 5-year OS rates varied between 50% and 73%. There were no significant differences in the rates of RFS (HR: 0.97; 95% CI: 0.74–1.19; p = .945) and OS (HR: 1.01; 95% CI: 0.66–1.36; p = .774) between the extended resection group and tumour resection alone group.

Conclusions

With adequate preparation and proper management, for patients with RPSs involving major vessels, aggressive surgical approach with vascular resection can achieve R0/R1 resection and improve survival.