bluuebunny/crossref_metadata_2025_split huggingface dataset:
The unique interplay of mitochondrial oxidative phosphorylation (OXPHOS) and immunity and its potential implication for the sex‐ and age‐related morbidity of severe COVID‐19 patients
Authors: Li Yinchuan , Li Lei , Wu Guanghao , Xie Gangcai , Yi Lirong , Zhu Jie , Liang ShiYu , Huang Ya‐ru , Chen Juan , Ji Shaoyang , Sun Fei , Liu Rui‐tian 
Published: September 2023

AbstractAged male patients are more vulnerable to severe or critical symptoms of COVID‐19, but the underlying mechanism remains elusive. In this study, we analyzed previously published scRNA‐seq data from a large cohort of COVID‐19 patients, castrated and regenerated mice, and bulk RNA‐seq of a RNAi library of 400 genes, and revealed that both immunity and OXPHOS displayed cell‐type‐, sex‐, and age‐related variation in the severe or critical COVID‐19 patients during disease progression, with a more prominent increase in immunity and decrease in OXPHOS in myeloid cells in the males relative to the females (60–69 years old). Male severe or critical patients above 70 years old were an exception in that the compromised negative correlation between OXPHOS and immunity in these patients was associated with its disordered transcriptional regulation. Finally, the expression levels of OXPHOS and androgens were revealed to be positively correlated, and the responses of macrophages to android fluctuation were more striking than other types of detected immune cells in the castrated mice model. Therefore, the interplay of OXPHOS and immunity displayed a cell‐type‐specific, age‐related, and sex‐biased pattern, and the underlying potential regulatory role of the hormonal milieu should not be neglected.
Ginsenoside RG1‐induced mesenchymal stem cells alleviate diabetic cardiomyopathy through secreting exosomal circNOTCH1 to promote macrophage M2 polarization
Authors: Zhen Juan , Bai Jinping , Liu Jia , Men Hongbo , Yu Haitao 
Published: September 2023

AbstractDiabetic cardiomyopathy (DCM) is a cardiac complication resulting from long‐term uncontrolled diabetes, characterized by myocardial fibrosis and abnormal cardiac function. This study aimed at investigating the potential of ginsenoside RG1 (RG1)‐induced mesenchymal stem cells (MSCs) in alleviating DCM. A DCM mouse model was constructed, and the effects of RG1‐induced MSCs on myocardial function and fibrosis in diabetic mice were evaluated. RG1‐induced MSCs were cocultured with high glucose‐treated fibroblasts for subsequent functional and mechanism assays. It was discovered that RG1‐induced MSCs secrete exosomes that induce macrophage M2 polarization. Mechanistically, exosomes derived from RG1‐induced MSCs transferred circNOTCH1 into macrophages, activating the NOTCH signaling pathway. A competing endogenous RNA (ceRNA) regulatory axis consisting of circNOTCH1, miR‐495‐3p, and NOTCH1 was found to contribute to DCM alleviation.. This study unveiled that exosomal circNOTCH1 secreted by RG1‐induced MSCs can alleviate DCM by activating the NOTCH signaling pathway to induce macrophage M2 polarization. This finding may contribute to the development of new therapeutic approaches for DCM.
A successful case of robot‐assisted thoracic surgery for apical thoracic schwannoma
Authors: Oyamatsu Hironori , Fujimura Takaki , Okagawa Takehiko , Niimi Seijirou 
Published: September 2023

Key messageThe thoracic apex is a narrow and complicated area with nerves and vessels, making it difficult to secure a visual field and perform surgical operations. Robot‐assisted surgery enabled good visibility and highly flexible forceps manipulation, we were able to perform minimally invasive and safe thoracic apical tumour resection.
Deep neuromuscular block attenuates intra‐abdominal pressure and inflammation and improves post‐operative cognition in prostate cancer patients following robotic‐assisted radical prostatectomy
Authors: Hu Guangjun , Shao Weidong , Chen Zhuo , Li Bixi , Xu Bo 
Published: September 2023

AbstractBackgroundWhether neuromuscular block (NMB) affects Intra‐abdominal pressure (IAP) and cognition in Prostate cancer (PC) patients with Robotic‐assisted laparoscopic radical prostatectomy (RALRP) remains unclear. Here we aimed to compare the effects of deep and moderate NMB on the IAP, inflammation, and cognition.MethodsThe Moderate neuromuscular block (MNMB) group (N = 44) and Deep neuromuscular block (DNMB) group (N = 47) were recruited. Intra‐abdominal pressure was adjusted to meet RALRP requirements. The expression of pro‐inflammatory factors was measured by ELISA. MMSE scores were recorded before the operation, 1 day and 1 week after the operation.ResultsSignificant decreases in IAP (p < 0.001) and IL‐1β, IL‐6, TNF‐a, and S‐100β (p ≤ 0.01) expressions were found in the DNMB group. The MMSE score in the DNMB group was higher than that in the MNMB group on day one (p = 0.046). The incidence of nausea and vomiting was lower in the DNMB group than that in the MNMB group (p = 0.013).ConclusionsDNMB reduces IAP and inflammation and improves post‐operative cognitive functions in PC patients with RALRP.
Interdisciplinary collaboration in higher education towards sustainable development
Authors: Podgórska Marzena , Zdonek Iwona 
Published: September 2023

AbstractThe literature and practitioners both emphasise the need to involve universities in the promotion and implementation of SDGs. On the other hand, the lack of practical solutions to include SDGs in university activity is constantly emphasised. The objective of the article is to investigate how project‐based learning innovations used in student education affect the achievement of sustainable development goals. We analyse whether innovative project based–learning projects at a leading technology university in Poland pursued from 2018 to 2021 contribute to achieving sustainable development goals, and we study the matter of interdisciplinarity of the project teams. To this end, we employ a qualitative content analysis of reports for 108 projects that involved 324 researchers, 607 students, and 233 experts from the university's business environment. The categorical data yielded by the analysis are analysed with quantitative methods: frequency analysis, correspondence analysis, and data visualisation. The analyses demonstrated that the most commonly pursued sustainable development goals were: SDG 9: Industry Innovation and Infrastructure, SDG 3: Good Health and Well‐Being, and SDG 12: Responsible Production and Construction. Biomedical Engineering experts pursued projects dedicated to SDG 3: Good Health and Well‐Being, while Automation and Robotics specialists focused on SDGs 9: Industry Innovation and Infrastructure and SDG 11: Sustainable Cities and Communities. The primary sustainable development goal for Environmental Engineering teams was SDG 12: Responsible Consumption and Production followed by SDG 6: Clean Water and Sanitation and 7: Affordable and Clean Energy. The most common interdisciplinarity in the teams was of the second type: interdisciplinarity bringing together experts from two research areas. The article presents the results of research on the process of promoting sustainable development through project‐based learning implemented under an interdisciplinary collaboration of teams of university staff, students, and experts from businesses associated with the university. The results contribute to the academic debate on the ability of universities to teach, implement, and promote sustainable development and its goals.
The last Baru (Crocodylia, Mekosuchinae): a new species of ‘cleaver‐headed crocodile’ from central Australia and the turnover of crocodylians during the Late Miocene in Australia
Authors: Yates Adam M. , Ristevski Jorgo , Salisbury Steven W. 
Published: September 2023

AbstractBaru is a genus that includes several large mekosuchine crocodylians from the Oligo‐Miocene of Australia. Here we describe Baru iylwenpeny sp. nov. from a large sample of cranial bones from the Upper Miocene Alcoota Local Fauna of the Northern Territory. Baru iylwenpeny can be diagnosed by several autapomorphies that include, but are not limited to: extreme reduction of the pneumatic foramina associated with the diverticula that invade the bones of the suspensorium; and enlargement of the postcaniniform maxillary teeth resulting in crowding of the postcaniniform alveoli and a reduction in the number of maxillary alveoli to 12. The new species is the geologically youngest known member of the genus. While species of Baru are a ubiquitous component of Oligo‐Miocene crocodylian assemblages from northern Australia, they are absent from all known Plio‐Pleistocene sites, suggesting the extinction of the genus by the latest Miocene. We suggest that the marked taxonomic turnover of crocodylian assemblages between the Upper Miocene Alcoota Local Fauna and the Lower Pliocene is the result of a short period of severe aridity during the latest Miocene, causing widespread crocodylian extinctions across Australia, followed by the establishment of taxonomically novel crocodylian faunas in the Pliocene. This event mirrors similar contemporary losses of crocodylian diversity in Africa and South America, although these cases are partly driven by local tectonic events. It is likely that late Cenozoic global cooling also played a role in these extinctions.
Ultrasound appearance of decidualized non‐ovarian endometriotic lesions during pregnancy and after delivery
Authors: Zajicek M. , Berkowitz E. , Yulzari V. , Kassif E. , Burke Y. , Elizur S. , Inbar Y. , Zolti M. , Weisz B. , Soriano D. 
Published: September 2023

ABSTRACTObjectiveTo evaluate the changes in the ultrasound characteristics of decidualized non‐ovarian endometriotic lesions that occur during pregnancy and after delivery.MethodsThis was a prospective observational cohort study carried out at a single tertiary center between December 2018 and October 2021. Pregnant women with endometriosis underwent a standardized transvaginal ultrasound examination with color Doppler imaging once in every trimester and after delivery. Non‐ovarian endometriotic lesions were measured and evaluated by subjective semiquantitative assessment of blood flow. Lesions with moderate‐to‐marked blood flow were considered decidualized. The size and vascularization of decidualized and non‐decidualized lesions were compared between the gravid state and after delivery. Only patients with non‐ovarian endometriotic lesion(s) who underwent postpartum examination were included in the final analysis.ResultsOverall, 26 pregnant women with a surgical or sonographic diagnosis of endometriosis made prior to conception were invited to participate in the study, of whom 24 were recruited. Of those, 13 women with non‐ovarian endometriosis who attended the postpartum examination were included. In 7/13 (54%) cases, the lesion(s) were decidualized. In 4/7 (57%) women with decidualized lesion(s), the size of the largest lesion increased during pregnancy, while in 3/7 (43%), the size was unchanged. The size of non‐decidualized lesions did not change during pregnancy. On postpartum examination, only seven lesions were observed, of which three were formerly decidualized and four were formerly non‐decidualized. Lesions that were detected after delivery appeared as typical endometriotic nodules and were smaller compared with during pregnancy. The difference in maximum diameter between the gravid and postpartum states was statistically significant in decidualized lesions (P < 0.01), but not in non‐decidualized lesions (P = 0.09). The reduction in mean diameter was greater in decidualized compared with non‐decidualized lesions (P = 0.03).ConclusionsDecidualization was observed in 54% of women with non‐ovarian endometriotic lesion(s) and resolved after delivery. Our findings suggest that the sonographic features of decidualization, which might mimic malignancy, are pregnancy‐related and that expectant management and careful monitoring should be applied in these cases. Clinicians should be aware of the changes observed during pregnancy to avoid misdiagnosing decidualized lesions as malignancy and performing unnecessary surgery. © 2023 The Authors. Ultrasound in Obstetrics & Gynecology published by John Wiley & Sons Ltd on behalf of International Society of Ultrasound in Obstetrics and Gynecology.
Magnetic resonance imaging findings of the primitive neuroectodermal tumour in lumbosacral spinal cord in a cat
Authors: Itoi Takamasa , Kutara Kenji , Mitsui Ikki , Akashi Natsuki , Kanda Teppei , Sugimoto Keisuke , Shimizu Yuki , Yamazoe Kazuaki 
Published: September 2023

AbstractA 5‐year‐old, castrated, male domestic short‐haired cat presented with neurological deficits in the pelvic limbs, back pain and dysuria. Magnetic resonance imaging showed a mass lesion caudal to the L4 vertebrae. In addition, suspected haemorrhage was observed at the cranial aspect of the mass. There was no evidence to support the presence of extravertebral intrusion or vertebral body, osteolysis. Dorsal laminectomy and durotomy were performed to debulk the intraspinal mass. Histopathological and immunohistochemical assessment revealed a primitive neuroectodermal tumour (PNET). To our knowledge, this is the first report to describe the clinical and pathological features and imaging diagnosis of intraspinal PNET without extraspinal invasion in a cat.
Mesoscale cyclonic storms associated with tornadoes and localised wind damage in frontal rainbands
Authors: Clark M. R. 
Published: September 2023

AbstractTen cases are identified of an unusual type of mesoscale windstorm in the United Kingdom between 2006 and 2021. The windstorms, which occurred in association with frontal rainbands, produced wind damage swaths no more than 20–30km wide, but sometimes extending for several hundred kilometres, often with embedded tornadoes. Storm structure and evolution are explored for two cases using radar data, surface observations, a 1.5‐km gridlength model and damage reports obtained from social media platforms and post‐event damage surveys. Findings are summarised in the form of a conceptual model, and some questions are posed for future research.
Assessment of learning in simulator‐based arthroscopy training with the diagnostic arthroscopy skill score (DASS) and neurophysiological measures
Authors: Aksoy Mehmet Emin , Kocaoglu Baris , İzzetoglu Kurtulus , Agrali Atahan , Yoner Serhat Ilgaz , Polat Mert Deniz , Kayaalp Mahmut Enes , Yozgatli Tahir Koray , Kaya Alper , Becker Roland 
Published: September 2023

AbstractPurposeVirtual arthroscopic training has become increasingly popular. However, there is a lack of efficiency‐based tracking of the trainee, which may be critical for determining the specifics of training programs and adapting them for the needs of each trainee. This study aims to evaluate and compare the measures obtained with a non‐invasive neurophysiological method with The Diagnostic Arthroscopy Skill Score (DASS), a commonly used assessment tool for evaluating arthroscopic skills.MethodsThe study collected simulator performance scores, consisting of “Triangulation Right Hand”, “Triangulation Left Hand”, “Catch the Stars” and “Three Rings” and DASS scores from 22 participants (11 novices, 11 experts). These scores were obtained while participants underwent a structured program of exercises for the fundamentals of arthroscopic surgery training (FAST) and knee module using a simulator‐based arthroscopy device. During the evaluation, data on oxy‐hemoglobin and deoxy‐hemoglobin levels in the prefrontal cortex were collected using the Functional Near‐Infrared Spectroscopy (fNIRS) imaging system. Performance scores, DASS scores, and fNIRS data were subsequently analyzed to determine any correlation between performance and cortex activity.ResultsThe simulator performance scores and the DASSPart2 scores were significantly higher in the expert group compared to the novice group (200.1 ± 28.5 vs 172.5 ± 48.9, p = 0.04 and 9.4 ± 5.6 vs. 5.4 ± 5.6 p = 0.02). In the expert group, fNIRS data showed a significantly lower prefrontal cortex activation during fundamental tasks in the FAST module, indicating significantly more efficient mental resource use.ConclusionThe analysis of cognitive workload changes during simulation‐based arthroscopy training revealed a significant correlation between the trainees’ DASS scores and fNIRS data. This correlation suggests the potential use of fNIRS data and DASS scores as additional metrics to create adaptive training protocols for each participant. By incorporating these metrics, the training process can be optimized, leading to more efficient arthroscopic training and better preparedness for clinical operations.Level of evidenceIII.
Characterization and description of Gabonibacter chumensis sp. nov., isolated from feces of a patient with non-small cell lung cancer treated with immunotherapy
Authors: Diop Khoudia , Pidgeon Reilly , Diop Awa , Benlaïfaoui Myriam , Belkaid Wiam , Malo Julie , Bernet Eve , Veyrier Frederic , Jacq Maxime , Brun Yves , Elkrief Arielle , Castagner Bastien , Routy Bertrand , Richard Corentin 
Published: September 2023

AbstractA polyphasic taxonomic approach, incorporating analysis of phenotypic features, cellular fatty acid profiles, 16S rRNA gene sequences, and determination of average nucleotide identity (ANI) plus digital DNA–DNA hybridization (dDDH), was applied to characterize an anaerobic bacterial strain designated KD22T isolated from human feces. 16S rRNA gene-based phylogenetic analysis showed that strain KD22T was found to be most closely related to species of the genus Gabonibacter. At the 16S rRNA gene level, the closest species from the strain KD22T corresponded with Gabonibacter massiliensis GM7T, with a similarity of 97.58%. Cells of strain KD22T were Gram-negative coccobacillus, positive for indole and negative for catalase, nitrate reduction, oxidase, and urease activities. The fatty acid analysis demonstrated the presence of a high concentration of iso-C15: 0 (51.65%). Next, the complete whole-genome sequence of strain KD22T was 3,368,578 bp long with 42 mol% of DNA G + C contents. The DDH and ANI values between KD22T and type strains of phylogenetically related species were 67.40% and 95.43%, respectively. These phylogenetic, phenotypic, and genomic results supported the affiliation of strain KD22T as a novel bacterial species within the genus Gabonibacter. The proposed name is Gabonibacter chumensis and the type strain is KD22T (= CSUR Q8104T = DSM 115208 T).
Exterior Stability of Minkowski Space in Generalized Harmonic Gauge
Authors: Hintz Peter 
Published: September 2023

AbstractWe give a short proof of the existence of a small piece of null infinity for $$(3+1)$$

(
3
+
1
)

-dimensional spacetimes evolving from asymptotically flat initial data as solutions of the Einstein vacuum equations. We introduce a modification of the standard wave coordinate gauge in which all non-physical metric degrees of freedom have strong decay at null infinity. Using a formulation of the gauge-fixed Einstein vacuum equations which implements constraint damping, we establish this strong decay regardless of the validity of the constraint equations. On a technical level, we use notions from geometric singular analysis to give a streamlined proof of semiglobal existence for the relevant quasilinear hyperbolic equation.
Chemical composition, antioxidant profile and physicochemical properties of commercial non-cocoa- and cocoa-flavoured plant-based milk alternatives
Authors: Kasapidou Eleni , Basdagianni Zoitsa , Papatzimos Georgios , Papadopoulos Vasileios , Tsiftsi Ekaterini , Neki Irini , Nigianni Paraskevi-Anastasia , Mitlianga Paraskevi 
Published: September 2023

AbstractIn recent years, there has been a significant rise in the popularity of plant-based milk alternatives (PBMAs). This work examined the nutritional value, antioxidant profile and physicochemical characteristics of commercial non-cocoa- and cocoa-flavoured PBMAs. The examined products were either nut or oat-based ones. In the absence of protein fortification, the products' protein content was found to be low. Fat content was also low, whereas carbohydrate and sugar contents were related to sugar addition. Oleic, linoleic and α-linolenic acids were the primary unsaturated fatty acids. PBMAs have low Atherogenicity (AI) and Thrombogenicity (TI) indices and high hypocholesterolaemic:hypercholesterolaemic fatty acid ratio (h/H). The antioxidant profile significantly differed (P < 0.001) between non-cocoa- and cocoa-flavoured PBMAs. The antioxidant profile of cocoa-flavoured PMBAs improved due to the polyphenols present in cocoa beans. The physicochemical characteristics of the analysed PBMAs demonstrated great versatility. The nutritional profile of PBMAs also showed considerable variability, influenced by factors, such as product type and ingredient formulation. Additionally, differences in the nutritional composition and physicochemical properties were observed between non-cocoa and cocoa-based PBMAs.
On a microlocal version of Young’s product theorem
Authors: Dappiaggi Claudio , Rinaldi Paolo , Sclavi Federico 
Published: September 2023

AbstractA key result in distribution theory is Young’s product theorem which states that the product between two Hölder distributions $$u\in \mathcal {C}^\alpha (\mathbb {R}^d)$$

u
∈

C

α

(

R

d

)

and $$v\in \mathcal {C}^\beta (\mathbb {R}^d)$$

v
∈

C

β

(

R

d

)

can be unambiguously defined if $$\alpha +\beta >0$$

α
+
β
>
0

. We revisit the problem of multiplying two Hölder distributions from the viewpoint of microlocal analysis, using techniques proper of Sobolev wavefront set. This allows us to establish sufficient conditions which allow the multiplication of two Hölder distributions even when $$\alpha +\beta \le 0$$

α
+
β
≤
0

.
Targeting GD2 after allogeneic SCT: effector cell composition defines the optimal use of ch14.18 and the bispecific antibody construct NG-CU (GD2-CD3)
Authors: Arendt A.-M. , Heubach F. , Maier C. P. , Giardino S. , Jung G. , Kowalewski E. , Rabsteyn A. , Amorelli G. , Seitz C. , Schlegel P. , Handgretinger R. , Lang P. 
Published: September 2023

AbstractWe investigated whether T cell-recruiting bispecific anti-CD3/GD2 antibody NG-CU might be an alternative to therapeutic anti-GD2 monoclonal antibody (mAb) ch14.18, mediating complement-dependent cytotoxicity (CDC) and antibody-dependent cell-mediated cytotoxicity (ADCC) through natural killer (NK) cells for immunotherapy in high-risk/relapsed neuroblastoma after autologous/allogeneic stem cell transplantation (auto/alloSCT). Different antibody concentrations and effector-to-target ratios (E:T) were evaluated using xCELLigence RTCA system, peripheral blood mononuclear cells (PBMCs) (healthy donors and patients after alloSCT), and neuroblastoma cell lines (LS/LAN-1). Mean specific lysis of LS cells utilizing PBMCs from healthy donors and ch14.18 (1 µg/ml) was 40/66/75% after 12/24/48 h compared to 66/93/100% in the presence of NG-CU (100 ng/ml). NG-CU showed enhanced cytotoxicity compared to ch14.18, even at lower concentrations and E:T ratios, and completely eradicated LS cells after 72 h. To decipher the influence of effector cell subsets on lysis, different ratios of T and NK cells were tested. At a ratio of 1:1, ch14.18 was more effective than NG-CU. Using patient PBMCs taken at different time points posttransplant, significant lysis with both constructs was detectable depending on percentages and total numbers of T and NK cells; in the early posttransplant phase, NK cells were predominant and ch14.18 was superior, whereas later on, T cells represented the majority of immune cells and NG-CU was more effective. Our study highlights the importance of analyzing effector cell subsets in patients before initiating antibody-based therapy. Consequently, we propose an adjusted administration of both antibody constructs, considering the state of posttransplant immune recovery, to optimize anti-tumor activity.