bluuebunny/crossref_metadata_2025_split huggingface dataset:
Parazoanthus axinellae Extract Incorporated Hybrid Nanostructure and Its Potential Antimicrobial Activity**
Authors: Konuklugil Belma , Uras Ibrahim Seyda , Karsli Baris , Demirbas Ayse 
Published: July 2023

AbstractThis study, it was aimed to examine the change in the antimicrobial effect of sea anemone Parazoanthus axinellae extract by forming its nanoflowers. A scanning electron microscope (SEM) and energy dispersive X‐ray spectroscopy (EDX) were expended to observe the morphologies of the Cu NFs that had been produced. Fourier transform infrared spectroscopy (FT‐IR) and X‐ray diffraction (XRD) techniques were expended to analyze the managing assemblies in P. axinellae extract, which perform an effective part in the synthesis routine, as well as the crystal assembly of NFs.P. axinellae extract mediated the HNFs (Hybrid nanoflowers) are at high, pure crystalline nature, flower shape with a crystallographic system at the nanoscale with mean crystallite size 21.9 nm using XRD, and average particle size ~10 nm by SEM. The broad absorption band at 2981–2915 cm−1 in the FT‐IR spectra of anemone extract and Cu‐anemone NFs represents the unique peak of hydroxy groups.In addition, Cu NFs were tested for their antibacterial properties. Cu NFs have been discovered to exhibit antibacterial properties. It is suggested that P. axinellae extract and various inorganic components be used to synthesize a variety of NFs and assess their suitability for usage in biomedical fields.
Lycopodium Alkaloids from Huperzia serrata and Their Anti‐acetylcholinesterase Activities
Authors: Li Wen , Zhu Hui‐Hui , Shen Xi , Tan Jin‐Lin , Tang Qing , Ling Zhi‐Peng , Zhao Hai‐Yue , Lin Qiang , Sun Hui , Zhang Hai‐Peng , Li Yao‐Lan , Wang Guo‐Cai , Zhang Yu‐Bo 
Published: July 2023

AbstractOne new fawcettimine‐type alkaloid (1), one new miscellaneous‐type alkaloid (2), four new lycodine‐type alkaloids (3–6), and eight known ones (7–14) were isolated from the whole plants of Huperzia serrata. Their structures and absolute configurations were elucidated based on spectroscopic data, X‐ray diffraction, ECD calculation and Mosher's method. Compound 1 was a rare C18N2‐type Lycopodium alkaloid, possessing serratinine skeleton with an amide side chain in C‐5. The absolute configuration of the 18‐OH of compounds 4–6 were first determined by Mosher's method. Moreover, compounds 1–14 were assayed anti‐acetylcholinesterase effect in vitro, and compound 7 showed significant anti‐acetylcholinesterase activity with an IC50 value of 16.18±1.64 μM.
Spatially Resolved Chiroptical Spectroscopies Emphasizing Recent Applications to Thin Films of Chiral Organic Dyes
Authors: Albano Gianluigi , Taddeucci Andrea , Pescitelli Gennaro , Di Bari Lorenzo 
Published: July 2023

AbstractInstrumental techniques able to identify and structurally characterize the aggregation states in thin films of chiral organic π‐conjugated materials, from the first‐order supramolecular arrangement up to the microscopic and mesoscopic scale, are very helpful for clarifying structure‐property relationships. Chiroptical imaging is currently gaining a central role, for its ability of mapping local supramolecular structures in thin films. The present review gives an overview of electronic circular dichroism imaging (ECDi), circularly polarized luminescence imaging (CPLi), and vibrational circular dichroism imaging (VCDi), with a focus on their applications on thin films of chiral organic dyes as case studies.
Visible‐Light Enabled Dehydroxylative Alkylation of α‐Hydroxy Carboxylic Acid Derivatives via C−O Bond Cleavage
Authors: Liu Xiaobo , Lu Minming , Guo Xiangli , Xu Huajian , Xu Jun 
Published: July 2023

AbstractA novel visible‐light photoredox strategy is reported for the efficient dehydroxylative alkylation of a wide array of α‐hydroxy carboxylic acid derivatives using diaryl boron radical. The reaction features readily accessible starting materials, broad substrate scope with excellent functionality tolerance. Preliminary mechanistic studies reveal that the spin‐center shift process is responsible for the C−O bond activation, which is promoted by the diaryl boron radical generated from bench‐stable and commercially available tetraphenyl borate (NaBPh4).
Electronic Structure Modulation of Nanographenes for Second Order Nonlinear Optical Molecular Materials
Authors: Yang Cui‐Cui , Tian Wei Quan 
Published: July 2023

AbstractNanographenes (NGs) have drawn extensive attention as promising candidates for next‐generation optoelectronic and nonlinear optical (NLO) materials, owing to its unique optoelectronic properties and high thermal stability. However, the weak polarity or even non‐polarity of NGs (resulting in weak even order NLO properties) and the high chemical reactivity of zigzag edged NGs hinder their further applications in nonlinear optics, thus stabilization (lowering the chemical reactivity) and polarizing the charge distribution in NGs are necessary for such applications of NGs. The fusion of heptagon and pentagon endows the azulene with the character of donor‐acceptor, and the B=N unit is isoelectronic to C=C unit. The introduction of polar azulene and BN are idea to polarize and stabilize the electronic structure of NGs for NLO applications. In the present review, a survey on the functionalization and applications of NGs in nonlinear optics is conducted. The engineering of the electronic structure of NGs by topological defects, doping and edge modulation is summarized. Finally, a summary of challenges and perspectives for carbon‐based NLO nanomaterials is presented.
Research progress in water‐saving cultivation of rice in China
Authors: Jiang Hao , Xing Xu , Meng Xin , Chen Junlei , Yu Kuo , Xu Xiaotian , Zhang Rui , Wei Zhiheng , Wang Dongchao , Cang Baifeng , Bai Chenyang , Li Yunzhe , Jiang Zixian , Wei Xiaoshuang , Tian Ping , Yang Meiying , Wu Zhihai 
Published: July 2023

AbstractThe conflict between rice production and water scarcity is becoming more pronounced. Therefore, the advancement of water‐saving rice cultivation is crucial in guaranteeing both food and ecological security. This study provides a summary of the development and characteristics of various water‐saving cultivation techniques for rice, including alternate wetting and drying irrigation, wet irrigation, controlled irrigation, the system of rice intensification, direct seeding rice, and dry cultivation of rice (DCR). We also introduce the varietal characteristics of upland rice, aerobic rice, water‐saving and drought‐resistant rice, and DCR. We summarize the impact of different water‐saving cultivation models on yield and quality and regulatory measures, and propose challenges and strategies for the development of water‐saving cultivation of rice in the future. We hope to provide a reference for promoting the development of rice water‐saving cultivation and dry farming agriculture.
Course and outcome in individuals with atypical anorexia nervosa: Findings from the Study of Refeeding to Optimize iNpatient Gains (StRONG)
Authors: Golden Neville H. , Kapphahn Cynthia J. , Cheng Jing , Kreiter Anna , Downey Amanda E. , Accurso Erin C. , Machen Vanessa I. , Adams Sally H. , Buckelew Sara M. , Moscicki Anna‐Barbara , Le Grange Daniel , Garber Andrea K. 
Published: July 2023

AbstractObjectiveWe previously reported that participants with atypical anorexia nervosa (atypical AN) had higher historical and admission weights, greater eating disorder psychopathology, but similar rates of amenorrhea and weight suppression at baseline as compared to anorexia nervosa (AN); here, we compare 1‐year outcomes.MethodWeight, % median body mass index (%mBMI), Eating Disorder Examination Questionnaire (EDE‐Q) scores, resumption of menses, and rehospitalizations were examined at 3, 6, and 12 months post‐discharge. Analyses (N = 111) compared changes in %mBMI, weight suppression, and EDE‐Q scores over time between atypical AN and AN.ResultsAmong the participants (48 atypical AN, 63 AN), both groups gained weight but those with atypical AN had lower gains than those with AN in %mBMI (p = .02) and greater weight suppression (p = .002) over time. EDE‐Q scores improved over time, independent of weight suppression, with no significant difference between atypical AN and AN. Groups did not differ by rates of resumption of menses (80% atypical AN, 76.9% AN) or rehospitalization (29.2% atypical AN, 37.9% AN). Greater weight suppression predicted longer time to restore menses and more days of rehospitalization.DiscussionIndividuals with atypical AN regained a smaller proportion of body mass and were more weight suppressed over time. Change in eating disorder cognitions, resumption of menses, and rehospitalization rates at 1‐year follow‐up did not differ between groups. There was no significant difference in weight suppression between groups for those who were psychologically improved at 12 months. Findings highlight limitations in our understanding of weight recovery in atypical AN. New metrics for recovery are urgently needed.Public SignificanceLittle is known about outcome in atypical anorexia nervosa (atypical AN). We examined recovery metrics in young people with atypical AN and anorexia nervosa (AN) 1 year after medical hospitalization. Individuals with atypical AN showed slower weight gain and remained further from their pre‐illness weight. There were no differences in the rates of psychological recovery, resumption of menses, or rehospitalization. New metrics are needed to assess recovery in atypical AN.
Proteomics reveals a global phenotypic shift of NK cells in HCV patients treated with direct‐acting antivirals
Authors: Bi Wenjie , Kraft Anke , Engelskircher Sophie , Mischke Jasmin , Witte Moana , Klawonn Frank , van Ham Marco , Cornberg Markus , Wedemeyer Heiner , Hengst Julia , Jänsch Lothar 
Published: July 2023

AbstractChronic hepatitis C virus (HCV) infections compromise natural killer (NK)‐cell immunity. Direct‐acting antivirals (DAA) effectively eliminate HCV, but the long‐term effects on NK cells in cured patients are debated. We conducted a proteomic study on CD56+ NK cells of chronic HCV‐infected patients before and 1 year after DAA therapy. Donor‐variation was observed in NK‐cell proteomes of HCV‐infected patients, with 46 dysregulated proteins restored after DAA therapy. However, 30% of the CD56+ NK‐cell proteome remained altered 1 year post‐therapy, indicating a phenotypic shift with low donor‐variation. NK cells from virus‐negative cured patients exhibited global regulation of RNA‐processing and pathways related to “stimuli response”, “chemokine signaling”, and “cytotoxicity regulation”. Proteomics identified downregulation of vesicle transport components (CD107a, COPI/II complexes) and altered receptor expression profiles, indicating an inhibited NK‐cell phenotype. Yet, activated NK cells from HCV patients before and after therapy effectively upregulated IFN‐γ and recruited CD107a. Conversely, reduced surface expression levels of Tim‐3 and 2B4 were observed before and after therapy. In conclusion, this study reveals long‐term effects on the CD56+ NK‐cell compartment in convalescent HCV patients 1 year after therapy, with limited abundance of vesicle transport complexes and surface receptors, associated with a responsive NK‐cell phenotype.
Quantifying the Electronic Effect of C4 and C4′ Positions of Bipyridine Ligand on Pd(II)/Rh(I)‐Catalyzed Conjugate Addition Reaction: A DFT Study
Authors: Jie Yao , Zhang Wei , Guo Jing‐Yi , Lu Hao , Song Hui‐Ting , Xu Xin‐Yu , Yan Hong 
Published: July 2023

AbstractThe electronic effects of the bidentate ligands play a vital role in the transition metal‐catalyzed conjugate addition reactions. Here, the insertion step (rate‐determining step (RDS) of the conjugate addition) catalyzed by Pd(II)/Rh(I)‐complexes with 26 bipyridine‐type (bpy) ligands linking different substituent groups in the opposite sides (C4, C4′ position) are systematically studied by density functional theory (DFT). It is found that for both Pd(II)‐ and Rh(I)‐catalysis, the stronger the electron‐withdrawing group connecting to both C4 and C4′ positions of bpy ligands can promote the insertion step. The predominance of π‐back donation in Rh(I) and σ‐donation in Pd(II) is the main reason for above different electronic properties of Pd(II) and Rh(I)‐catalysis. This work gives enough theoretical guide to the rational design of the efficient transition metal‐based catalyst for conjugate addition.
MYT1L variant inherited by a mosaic father in a case of severe developmental and epileptic encephalopathy
Authors: Boeri Silvia , Scala Marcello , Madia Francesca , Perucco Francesca , Vozzi Diego , Capra Valeria , Zara Federico , Nobili Lino , Mancardi Maria Margherita 
Published: July 2023

AbstractThe MYT1L gene plays a critical role in brain development, promoting the differentiation and proliferation of cells, important for the formation of brain connections. MYT1L is also involved in regulating the development of the hypothalamus, which is a crucial actor in weight regulation. Genetic variants in the MYT1L are associated with a range of developmental disorders, including intellectual disability, autism spectrum disorder, facial dysmorphisms, and epilepsy. The specific role of MYT1L in epilepsy remains elusive and no patients with developmental and epileptic encephalopathy (DEE) have been described so far. In this study, we report a patient with DEE presenting with severe refractory epilepsy, obesity, and behavioral abnormalities. Exome sequencing led to the identification of the heterozygous variant NM_001303052.2: c.1717G>A, p.(Gly573Arg) (chr2‐1910340‐C‐T; GRCh38.p14) in the MYT1L gene. This variant was found to be inherited by the father, who was a mosaic and did not suffer from any neuropsychiatric disorders. Our observations expand the molecular and phenotype spectrum of MYT1L‐related disorders, suggesting that affected individuals may present with severe epileptic phenotype leading to neurocognitive deterioration. Furthermore, we show that mosaic parents may not display the disease phenotype, with relevant implications for genetic counseling.
Protein biomarker for early diagnosis of microbial toxin contamination: Using Aspergillus flavus as an example
Authors: Fu Jiayun , Gu Mei , Yan Honglin , Zhang Minhui , Xie Huali , Yue Xiaofeng , Zhang Qi , Li Peiwu 
Published: July 2023

AbstractMicrobial toxins are important factors that contribute to human diseases and food losses. Predicting the production of these toxins before they occur is a significant challenge. In this study, we innovatively developed a strategy called targeted acquisition of toxin‐related immunogen (TATRI) to identify early diagnostic biomarkers for microbial toxins. Taking Aspergillus flavus as an example, we employed an immunoassay‐based screening approach to identify nanobodies that exhibited significant response signals to different toxigenic strains. Subsequently, we isolated and purified the specific immunogen pre‐aflatoxin biomarker PAB‐01 that bound to the selected nanobody. The results showed that the level of PAB‐01 was positively correlated with the toxin‐producing ability of A. flavus strains and could be detected before the production of aflatoxins. It met the essential requirements as an early diagnostic biomarker for aflatoxin contamination. Protein identification and bioinformatics analysis revealed that PAB‐01 is a glycoprotein that can be secreted into the extracellular space. It is located on chromosome 5 of A. flavus and belongs to the 75 family of glycoside hydrolases. Furthermore, we used 7 g/kg as the critical concentration of PAB‐01 to diagnose the risk of aflatoxin contamination in stored peanuts, with an accuracy rate of 75.9% for high‐risk samples. In summary, this work provides a promising approach for the identification of protein biomarkers prior to microbial toxin production and the establishment of early diagnostic techniques for microbial toxins.
Investigation of the effects of different processing methods on the selected nutritional properties of pumpkin and determining the appropriate process for pumpkin yogurt
Authors: Sıçramaz Hatice , Ayar Ahmet 
Published: July 2023

AbstractThe processing methods, especially cooking, can cause quality losses, particularly in the nutritional value of the fresh product. This study investigated the effects of preprocessing methods on the nutritional properties of pumpkin and the physicochemical and sensory properties of pumpkin yogurt. Two different pumpkin varieties (Cucurbita pepo and Cucurbita maxima) were subjected to three different preprocessing methods (freeze‐drying, boiling, and baking). Boiling significantly increased antioxidant activity (p ≤ .05), followed by baking. C. maxima had higher TDF and TPC than C. pepo, but in both pumpkin varieties, TDF did not change with heat treatment (boiling and baking), while TPC decreased. Mineral contents remained the same or decreased with heat treatment, except for Mn and Fe. In particular, the addition of C. maxima significantly affected the color parameters (L*, a*, b*) of yogurt and improved WHC (from 68.9% to 91.6%) and hardness (from 58.0 to 193.5 g; p ≤ .05). The sensory evaluation concluded that heat‐treated (boiled and baked) samples were preferred more than freeze‐dried raw ones. In conclusion, the results revealed that adding boiled and baked pumpkins, especially the preference for C. maxima instead of C. pepo, improved the quality parameters of yogurt.
Altered dynamic brain activity and functional connectivity in thyroid‐associated ophthalmopathy
Authors: Jiang Wen‐Hao , Liu Jun , Zhou Jiang , Wu Qian , Pu Xiong‐Ying , Chen Huan‐Huan , Xu Xiao‐Quan , Wu Fei‐Yun , Hu Hao 
Published: July 2023

AbstractAlthough previous neuroimaging evidence has confirmed the brain functional disturbances in thyroid‐associated ophthalmopathy (TAO), the dynamic characteristics of brain activity and functional connectivity (FC) in TAO were rarely concerned. The present study aims to investigate the alterations of temporal variability of brain activity and FC in TAO using resting‐state functional magnetic resonance imaging (rs‐fMRI). Forty‐seven TAO patients and 30 age‐, gender‐, education‐, and handedness‐matched healthy controls (HCs) were enrolled and underwent rs‐fMRI scanning. The dynamic amplitude of low‐frequency fluctuation (dALFF) was first calculated using a sliding window approach to characterize the temporal variability of brain activity. Based on the dALFF results, seed‐based dynamic functional connectivity (dFC) analysis was performed to identify the temporal variability of efficient communication between brain regions in TAO. Additionally, correlations between dALFF and dFC and the clinical indicators were analyzed. Compared with HCs, TAO patients displayed decreased dALFF in the left superior occipital gyrus (SOG) and cuneus (CUN), while showing increased dALFF in the left triangular part of inferior frontal gyrus (IFGtriang), insula (INS), orbital part of inferior frontal gyrus (ORBinf), superior temporal gyrus (STG) and temporal pole of superior temporal gyrus (TPOsup). Furthermore, TAO patients exhibited decreased dFC between the left STG and the right middle occipital gyrus (MOG), as well as decreased dFC between the left TPOsup and the right calcarine fissure and surrounding cortex (CAL) and MOG. Correlation analyses showed that the altered dALFF in the left SOG/CUN was positively related to visual acuity (r = .409, p = .004), as well as the score of QoL for visual functioning (r = .375, p = .009). TAO patients developed abnormal temporal variability of brain activity in areas related to vision, emotion, and cognition, as well as reduced temporal variability of FC associated with vision deficits. These findings provided additional insights into the neurobiological mechanisms of TAO.
Financial issues in times of a COVID‐19 pandemic in a tertiary hospital in Mali
Authors: Ridde Valéry , Coulibaly Abdourahmane , Touré Laurence , Ba Mouhamadou Faly , Zinszer Kate , Bonnet Emmanuel , Honda Ayako 
Published: July 2023

AbstractBackgroundThis study examines how the functioning of healthcare providers during the COVID‐19 pandemic was affected by the government financing response, which was shaped by existing healthcare financing systems.MethodsThe study applied a single case study design at a tertiary hospital in Bamako during the 1st and 2nd waves of the COVID‐19 pandemic. Data were gathered through 51 in‐depth interviews with hospital staff, participatory observation, and reviewing media articles and hospital financial records.ResultsThe study revealed the disruptions experienced by hospital managers, human resources for health and patients in Mali during the early stages of the pandemic. While the government aimed to support universal access to COVID‐19‐related services, efforts were undermined by issues associated with complex public financing management procedures. The hospital experienced long delays in transferring government funds. The hospital suffered a decrease in revenue during the early stages of the pandemic. Government budgets were not effectively used because of complex, non‐agile procedures that could not adapt to the emergency. The challenges faced by the hospitals led to the delays in the staff payments of salaries and promised bonuses, which created potential for unfair treatment of patients. Excluding some COVID‐19 related items from the government funded benefit package created a financial burden on people receiving services. The managerial challenges experienced in the study hospital during the first wave continued in the second wave.ConclusionsPre‐existent issues in healthcare financing and governance constrained the effective management of COVID‐19‐related services and created confusion at the front line of healthcare service delivery.
Global research publications on cardiovascular and pulmonary rehabilitation and their alignment with population rehabilitation needs: An ecological study
Authors: Reboredo Maycon Moura , Erothildes Ferreira Renato , Bainha Ana Clara Cattete , Correia Tiago , Jesus Tiago S. 
Published: July 2023

AbstractPurposeTowards informing health research policy and planning, this article evaluates the relationship of the research publications in cardiac rehabilitation (CR) and pulmonary rehabilitation (PR) with the rehabilitation needs arising from cardiovascular diseases (except stroke) and chronic respiratory diseases, over time (1990–2017).MethodsEcological study using a secondary analysis and linear regressions of public domain data to associate two datasets of population‐level data: (1) research publications for CR and PR (data from the PubMed database); and (2) global need for CR and PR (data from the Global Burden of Disease study).ResultsThe percentage of both CR and PR publications (among total rehabilitation research) significantly decreased from 1990 to 2017 (both: p < 0.01). PR needs and research publications were aligned: around 5% of total rehabilitation needs and rehabilitation research. For CR needs (around 2%, but significantly increasing since 1990), we found a greater portion of CR research publications (6.5% or over). Finally, we found an inverse association among the percentage of CR research publications and CR needs (b = −6.08; r2 = 0.37, p = 0.001).ConclusionThe portion of CR and PR research (among total rehabilitation research) is declining over time. Yearly percentage of CR publications were greater than those of PR but for lower level of rehabilitation need, but the disparate trend was significantly decreasing over time. Population rehabilitation needs and their alignment with research volume must be one factor in the design of population‐centred, equitable health research priorities.