bluuebunny/crossref_metadata_2025_split huggingface dataset:
Lower accuracy of testosterone, cortisol, and free T4 measurements using automated immunoassays in people undergoing hemodialysis
Authors: Jansen Heleen I. , van Herwaarden Antonius E. , Huijgen Henk J. , Vervloet Marc G. , Hillebrand Jacquelien J. , Boelen Anita , Heijboer Annemieke C. 
Published: March 2023

Abstract

Objectives
Hormone measurements using automated immunoassays (IAs) can be affected by the sample matrix. Liquid chromatography tandem-mass spectrometry (LC-MS/MS) is less affected by these matrix effects. In clinical laboratories, testosterone, cortisol and, free thyroxine (FT4) are often measured using IAs. Renal failure alters serum composition in blood samples from people undergoing hemodialysis (HDp) and have, therefore, a complex serum constitution compared to healthy controls (HC). The goal of this study was to investigate the accuracy of testosterone, cortisol, and FT4 measurements in samples of HDp and to get more insight in the interfering factors.

Methods
Thirty serum samples from HDp and HC were collected to measure testosterone, cortisol, and FT4 using a well standardized isotope dilution (ID)-LC-MS/MS method and 5 commercially available automated IAs (Alinity, Atellica, Cobas, Lumipulse, UniCel DXI). Method comparisons between LC-MS/MS and IAs were performed using both HDp and HC samples.

Results
Average bias from the LC-MS/MS was for testosterone, cortisol, and FT4 immunoassays respectively up to 92, 7–47 and 16–27% more in HDp than in HC samples and was IA dependent. FT4 IA results were falsely decreased in HDp samples, whereas cortisol and testosterone concentrations in females were predominantly falsely increased. Correlation coefficients between LC-MS/MS and IA results were lower in HDp compared to HC samples.

Conclusions
Several IAs for testosterone (in women), cortisol, and FT4 are less reliable in the altered serum matrix of samples of HDp than in HC. Medical and laboratory specialists should be aware of these pitfalls in this specific population.
An unexpected case of neonatal compartment syndrome associated with congenital anomalies of kidney and urinary tract
Authors: Seske Laura M. , Mastroianni Melissa , Aziz Keith T. , Lewallen Laura W. 
Published: March 2023

Abstract

Objectives
Neonatal compartment syndrome (NCS) occurs when increased pressure within the fasciocutaneous compartment decreases capillary perfusion, causing irreversible tissue damage from ischemia. NCS is a rare condition that requires prompt diagnosis and treatment. Diagnosing NCS is highly dependent on the examination, which can be difficult in newborns. Prompt recognition provides the best chance for good outcomes.

Case presentation
We present a case of NCS diagnosed and treated based on physical examination findings. Fetal ultrasonography showed bladder distension, bilateral hydroceles, urethra dilation, and abdominal urinary ascites concerning for lower urinary tract obstruction and possible bladder rupture. At 1 h after birth, examination of the infant’s left upper extremity showed no spontaneous movement, the hand and forearm appeared dusky, and the hand had a large blister with desquamation. No pulse distal to the antecubital fossa was detected via Doppler ultrasonography. The infant was diagnosed with NCS and underwent urgent fasciotomy. The clinical appearance and perfusion of the left upper extremity gradually improved. At four months of age, the wounds were healed and the patient had full passive range of motion of the left upper extremity. Recovery of active motion is ongoing.

Conclusions
The presence of blistering and desquamation should provoke suspicion for NCS. Once NCS is diagnosed, prompt intervention is necessary to reduce the risk of poor functional outcomes. This case highlights the need for increased awareness of the risk developing compartment syndrome in utero as part of the rare sequalae in infants with congenital anomalies of kidney and urinary tract.
Confinement of ozone hole mainly in the Antarctic stratosphere to protect the living kingdom on the earth: chemistry behind this Nature’s unique gift
Authors: Das Udita , Das Ankita , Das Asim K. 
Published: March 2023

Abstract
Man-made activities can release the ozone depleting substances (ODSs) like chlorofluorocarbons (CFCs) and other halocarbons stable in atmosphere and ultimately, they migrate to the stratosphere where they can destroy the ozone layer through the XOx catalytic cycle (X = Cl, Br). The active forms in this catalytic cycle are X and XO that can be arrested in the inactive forms like XONO2 (halogen nitrate, an additive compound of two odd electron molecules XO and NO2) and HX (produced in the reaction of X with CH4) in the stratosphere to prevent the ozone depletion cycle. The catalytically active forms from these inactive species can be regenerated in the reactions on heterogeneous solid surface like polar stratospheric cloud (specially Type II PSC formed at about −85 °C). Formation of such PSC in the stratosphere is only possible in the supercooled stable Antarctic vortex produced in the prolonged winter. In fact, formation of such PSC in the stratosphere is not possible in the other regions of the earth and not even in the Arctic pole where no stable Arctic vortex is generally formed in the winter. Thus nature confines the ozone depletion reactions mainly in the stratosphere of Antarctica pole which is practically inhabited.
Analysis of common biomarkers in capillary blood in routine clinical laboratory. Preanalytical and analytical comparison with venous blood
Authors: Maroto-García Julia , Deza Sara , Fuentes-Bullejos Patricia , Fernández-Tomás Patricia , Martínez-Espartosa Débora , Marcos-Jubilar María , Varo Nerea , González Álvaro 
Published: March 2023

Abstract

Objectives
Remote self-collected capillary blood samples have been proposed as alternative to venous blood samples as an aid in telemedicine. The aim of this work is to compare the preanalytical and analytical performance of these two types of samples and to study the stability of common measurands in capillary blood.

Methods
Capillary and venous blood samples were collected in parallel from 296 patients in serum tubes to analyze 22 common biochemistry magnitudes after centrifugation and in EDTA tubes to analyze 15 hematologic magnitudes. Quality of the preanalytical process was assessed applying the model of quality indicator. 24 h stability at room temperature was studied by obtaining paired capillary samples. A questionnaire of assessment was conducted.

Results
Mean hemolysis index was higher in capillary samples compared to venous blood samples (p<0.001). Regression analysis and difference analysis showed no bias for all studied biochemistry parameters and hematologic parameters, except mean corpuscular volume (MCV), between capillary and venous blood samples. Regarding sample stability, percentage deviation was higher than the corresponding minimum analytical performance specification for ferritin, vitamin D, hematocrit, MCV, mean corpuscular hemoglobin concentration, platelets distribution wide, mean platelet volume and basophils. Finger pricking was perceived as less painful (p<0.05) than venipuncture in participants who undergo more than one blood test per year.

Conclusions
Capillary blood can be used as an alternative to venous blood for the studied parameters in automated common clinical analyzers. Cautious should be taken if samples are not analyzed within 24 h from the collection.
Soft sensor method of multimode BOF steelmaking endpoint carbon content and temperature based on vMF-WSAE dynamic deep learning
Authors: Yang Lu , Liu Hui , Chen Fugang 
Published: March 2023

Abstract
The difficulty of endpoint determination in basic oxygen furnace (BOF) steelmaking lies in achieving accurate real-time measurements of carbon content and temperature. For the characteristics of serious nonlinearity between process data, deep learning can perform excellent nonlinear feature representation for complex structural data. However, there is a process drift phenomenon in BOF steelmaking, and the existing deep learning-based soft sensor models cannot adapt to changes in the characteristics of samples, which may lead to their performance degradation. To deal with this problem, considering the characteristics of multimode distribution of process data, an adaptive updating deep learning model based on von-Mises Fisher (vMF) mixture model and weighted stacked autoencoder is proposed. First, the stacked autoencoder (SAE) and vMF mixture model are constructed for complex structural data, which can initially establish nonlinear mapping relationships and division of different distributions. Second, for each query sample, the basic SAE network will perform online adaptive fine-tuning according to its data with the same distribution to achieve dynamic updating. Moreover, each sample is assigned a weight according to its similarity with the query sample. Through the designed weighted loss function, the updated deep network will better match the working conditions of the query sample. Experimental studies with numerical examples and actual BOF steelmaking process data are provided to demonstrate the effectiveness of the proposed method.
Generalized Onsager fluxes based on inexact differential 1-form
Authors: Yang Qiang , Leng Kuangdai , Zhang Man , Liu Yaoru 
Published: March 2023

Abstract
Onsager fluxes proposed by D.G.B. Edelen assume that the same symmetry, nonlinear Onsager reciprocal relations, holds near and far from equilibrium. This assumption leads to exact differential 1-form J ⋅ dX everywhere, where J and X are thermodynamic fluxes and forces, respectively. However, thermodynamic fluxes far from equilibrium are characterized by symmetry breaking, which lead to the inexact differential 1-form. It is shown in this paper that the inexact differential 1-form J ⋅ dX should be represented by multiple independent scalar-valued functions. Generalized Onsager fluxes are obtained based on such representation. Generalized Onsager fluxes do not satisfy the nonlinear Onsager reciprocal relations and contain multiple independent scalar-valued functions, so they are suitable to thermodynamic fluxes far from equilibrium. Generalized Onsager fluxes embody Onsager fluxes as a special case. Therefore, generalized Onsager fluxes provide a unified framework for thermodynamic fluxes near and far from equilibrium.
Concentration levels and risk assessment of organochlorine and organophosphate pesticide residue in selected cereals and legumes sold in Anambra State, south-eastern Nigeria
Authors: Omokpariola Patrick Leonard , Okoye Patrice A. C. , Okechukwu Victor U. , Omokpariola Daniel Omeodisemi 
Published: March 2023

Abstract
The levels of organochlorine and organophosphate pesticide residues in selected cereal crops (beans, cowpea, millet, maize, sorghum, and rice) purchased from major markets in Anambra, south-eastern Nigeria, were assessed and compared with established MRLs. The QuEChERS (quick, easy, cheap, effective, rugged, and safe) method was used for extraction and clean-up of pesticide residues. Thereafter detection and quantification were done using GC/MS. The result reveals that the analysed grain samples contained some organochlorine pesticides and organophosphates. The organochlorine was most dominant followed by the organophosphates. Organochlorine pesticide residues varied from 0.048 to 0.298 mg/kg in beans, BDL to 0.398 mg/kg in cowpea, 0.018–0.337 mg/kg in maize, 0.023–0.375 mg/kg in millet, 0.058–0.415 mg/kg in sorghum and 0.045–0.442 mg/kg in rice while organophosphate pesticide residue varied from BDL to 0.315 mg/kg in beans, BDL to 0.113 mg/kg in cowpea, BDL to 0.228 mg/kg in maize, BDL to 0.253 mg/kg in millet, BDL to 0.218 mg/kg in sorghum and BDL to 2.1 35 mg/kg in rice. Highest concentration of endosulphan II (0.442 mg/kg) was detected in rice, followed by aldrin (0.415 mg kg−1) in sorghum and endosulphan II (0.40 mg/kg) in sorghum. The pesticide toxicity index (PTI) was above one (1), whereas health index (HI) was less than one (1) and cancer risk were within USEPA reference guideline for crops indicating children will have greater health effect than adults. Hence, strict monitoring and control of pesticide residues in agricultural products is advocated.
GRM2 Regulates Functional Integration of Adult-Born DGCs by Paradoxically Modulating MEK/ERK1/2 Pathway
Authors: Ma Jiao , Hu Zhechun , Yue Huimin , Luo Yujian , Wang Chao , Wu Xuan , Gu Yan , Wang Lang 
Published: March 2023

Metabotropic glutamate receptor 2 (GRM2) is highly expressed in hippocampal dentate granule cells (DGCs), regulating synaptic transmission and hippocampal functions. Newborn DGCs are continuously generated throughout life and express GRM2 when they are mature. However, it remained unclear whether and how GRM2 regulates the development and integration of these newborn neurons. We discovered that the expression of GRM2 in adult-born DGCs increased with neuronal development in mice of both sexes. Lack of GRM2 caused developmental defects of DGCs and impaired hippocampus-dependent cognitive functions. Intriguingly, our data showed that knockdown ofGrm2resulted in decreased b/c-Raf kinases and paradoxically led to an excessive activation of MEK/ERK1/2 pathway. Inhibition of MEK ameliorated the developmental defects caused byGrm2knockdown. Together, our results indicate that GRM2 is necessary for the development and functional integration of newborn DGCs in the adult hippocampus through regulating the phosphorylation and activation state of MEK/ERK1/2 pathway.SIGNIFICANCE STATEMENTMetabotropic glutamate receptor 2 (GRM2) is highly expressed in mature dentate granule cells (DGCs) in the hippocampus. It remains unclear whether GRM2 is required for the development and integration of adult-born DGCs. We providedin vivoandin vitroevidence to show that GRM2 regulates the development of adult-born DGCs and their integration into existing hippocampal circuits. Lack of GRM2 in a cohort of newborn DGCs impaired object-to-location memory in mice. Moreover, we revealed that GRM2 knockdown paradoxically upregulated MEK/ERK1/2 pathway by suppressing b/c-Raf in developing neurons, which is likely a common mechanism underlying the regulation of the development of neurons expressing GRM2. Thus, Raf/MEK/ERK1/2 pathway could be a potential target for brain diseases related to GRM2 abnormality.
Solitary Fibrous Tumor of the Peritoneal Cavity and Greater Omentum: Case Report and Review of the Literature
Authors: Gendvilaitė Neda , Šeinin Dmitrij , Beržanskas Laurynas , Poškus Tomas 
Published: March 2023

Background: The solitary fibrous tumor is a rare soft tissue mesenchymal tumor which typically arises from the pleura but may be found anywhere in the body. Abdominal localizations are very rare. The solitary fibrous tumor is classified into two forms, pleural and extrapleural, and morphologically they resemble each other. The diagnostics of the tumor is usually accidental because usually there are no clinical symptoms. The main treatment of the solitary fibrous tumor is the surgical removal of the tumor while radiotherapy treatment and chemotherapy outcomes remain unclear due to the rarity of the tumor and lack of data.Case presentation: We report the case of the 32-year-old man who was diagnosed with the solitary fibrous tumor of the peritoneal cavity. Laparotomy was performed. A grayish-white, stiff, coarse-grained tumor about 11 cm in diameter of the greater omentum was found and radical omentectomy with tumor removal were performed. Postoperative course was uneventful and the patient is well with no signs of recurrence on the CT scan of the chest and abdomen and MRI of the pelvis at 6 months after surgery.Conclusions: The solitary fibrous tumor is a rare condition. It is a borderline-malignant tumor but may cause serious complications if not treated. Due to the absence of clinical symptoms, the tumor is usually detected accidentally. The radical surgical removal of the tumor is the most optimal treatment.
CHAC1 exacerbates LPS-induced ferroptosis and apoptosis in HK-2 cells by promoting oxidative stress
Authors: Zhou Zhihui , Zhang Hongwei 
Published: March 2023

Background: Sepsis-induced acute kidney injury (AKI) is a singularly grievous and life-threatening syndrome. Its pathogenesis is closely related to inflammatory response, apoptosis, oxidative stress, and ferroptosis. Cation transport regulator-like protein 1 (CHAC1), as a proapoptic factor, may be involved in apoptosis, oxidative stress, and ferroptosis. This study aimed to explore the role of CHAC1 in the lipopolysaccharide (LPS)-induced the human renal proximal tubular epithelial (HK-2) cells.
Methods: HK-2 cells were challenged with LPS to construct a model of sepsis-induced AKI in vitro. The role of CHAC1 in the LPS-induced HK-2 cells was explored using Western blot assay, cell counting kit-8 (CCK-8), flow cytometry, and colorimetric assays. Additionally, N-acetyl cysteine (NAC) was incubated with HK-2 cells to define deeply the relation between oxidative stress and apoptosis or ferroptosis.
Results: The expression of CHAC1 was enhanced in the kidney tissues of mice with sepsis--induced multiple organ dysfunction syndrome (MODS), through the Gene Expression Omnibus database (GSE60088 microarray dataset), and in the LPS-induced HK-2 cells. The cell viability was significantly reduced by LPS treatment, which was at least partly restored by the transfection of siCHAC1#1 and siCHAC1#2 but not siNC. In addition, down-regulation of CHAC1 counteracted the LPS-induced reactive oxygen species level and malonaldehyde concentrations while restored the LPS-induced glutathione concentrations. Meanwhile, interference of CHAC1 neutralized LPS-induced apoptosis rate, and the relative level of cleaved poly(ADP-ribose) polymerase (PARP)/PARP, and cleaved caspase-3/caspase-3. In addition, silencing of CHAC1 recovered the LPS-induced enhanced protein level of glutathione peroxidase 4 (GPx4) whereas antagonized the LPS-induced relative protein level of ACSL4 and that of iron. Moreover, application of NAC inverted the effect of CHAC1 on apoptosis and ferroptosis in HK-2 cells.
Conclusion: CHAC1 exacerbated ferroptosis and apoptosis by enhancing oxidative stress in LPS-induced HK-2 cells.
Paciente com lesões hipercrômicas e pruriginosas na região posterior do tórax
Authors: David Maura , Sgarbi Silveira Naiani , Fagundes de Oliveira Renan , Abegg Aline Cristina 
Published: March 2023

Qual seu diagnóstico?
Paciente feminina atendida no posto de saúde com queixas de lesões hipercrômicas e pruriginosas no dorso. Também relatou dor em coluna toracolombar e lombossacra de longa data. Realizou ressonancia que evidenciou compressão de raízes nervosas em alguns pontos, mas sem sinais de compressão medular. Analisando anamnese, exame fisico e exame de imagem a paciente foi diagnosticada com notalgia parestética.
Pengembangan Instrumen Penilaian Diri dan Sejawat Secara Online pada Materi Keterampilan Pukulan Lob Bulutangkis Mata Pelajaran PJOK di SMPN 21 Malang
Authors: Samsudin Aji , Fitriady Gema , Darmawan Arief , Januarto Oni Bagus 
Published: March 2023

Abstract: During the covid-19 pandemic, in the activities of Physical Education (PE) teachers at SMPN 21 Malang doing online learning there were obstacles experienced by teachers, namely giving assignments in the form of making motion skills videos so that students only did video assignments without understanding the concept of movement and assessment. student learning outcomes will take a lot of time because the teacher assesses manually one by one. This study aims to develop self- and peer-assessment instruments in the aspect of movement skills of the badminton bounce material. This research is a development by adopting the model from Borg and Gall with the steps of needs analysis, planning, initial product development, expert validation test phase and product refinement. Collecting data in the form of interviews with 2 PJOK teachers for needs analysis and questionnaires on 2 validators for expert validation. By using qualitative analysis techniques through reduction, presentation, and conclusion. The results of the study prove that the product of the assessment instrument can show that it is feasible to use with a little revision.
Abstrak: Pada saat pandemi covid-19 dalam aktivitas guru Pendidikan Jasmani Olahraga Kesehatan (PJOK) di SMPN 21 Malang melakukan pembelajaran secara daring ada kendala yang dialami oleh guru yaitu memberikan penugasan berupa pembuatan video keterampilan gerak sehingga siswa hanya mengerjakan tugas video tanpa memahami konsep gerakan dan penilaian hasil belajar siswa akan memakan banyak waktu karena guru menilai secara manual dengan satu - persatu. Penelitian ini bertujuan untuk mengembangkan instrumen penilaian diri dan sejawat dalam aspek keterampilan gerak materi pukulan melambung bulutangkis. Penelitian ini merupakan pengembangan dengan mengadopsi model dari Borg dan Gall dengan langkah analisis kebutuhan, perencanaan, pengembangan produk awal, tahap uji validasi ahli dan penyempurnaan produk. Pengumpulan data berupa wawancara pada 2 orang guru PJOK untuk analisis kebutuhan dan kuesioner pada 2 orang validator untuk validasi ahli. Dengan menggunakan teknik analisis kualitatif melalui reduksi, penyajian, dan kesimpulan. Hasil penelitian membuktikan produk instrumen penilaian dapat menunjukkan layak digunakan dengan sedikit revisi.
Pengembangan Ebook Model Latihan Whole Body Berbasis Video Analisis Pada Atlet Renang Persatuan Renang Seluruh Indonesia (PRSI) Kota Malang
Authors: Fauzi Ardhitya Zulfikar , Heynoek Febrita Paulina , Yudasmara Dona Sandy , Kurniawan Ari Wibowo 
Published: March 2023

Abstract: The exercises carried out by the swimming athletes of the All Indonesia Swimming Unit in Malang still tend to use drill exercises which tend to only repeat the movement without any variation, causing boredom, the need for separate exercises related to strength to increase the athlete's power. The purpose of this research and development is a variation of strength training with the whole body using an ebook with video analysis. This research method uses research and development (R and D). The instrument used in collecting data is to provide a questionnaire for needs analysis, evaluation of experts and for product testing. The data analysis technique used descriptive qualitative and quantitative analysis techniques. The results showed that the development of variations in strength training with the whole body using an ebook with video analysis can be applied with a validity level of 87.8 percent which is included in the very valid category. The conclusion of this research and development is a variety of wholebody strength training packaged into an ebook with a video analysis that is acceptable to the swimming athletes of the Indonesian Swimming Association (PRSI) Malang.
Abstrak: Latihan yang dilakukan oleh atlet renang Persatuan Renang Seluruh Indonesia Malang masih cenderung dengan latihan drill yang cenderung hanya mengulang gerakan tanpa adanya variasi sehingga menimbulkan kebosanan, perlunya latihan terpisah terkait kekuatan untuk meningkatkan power atlet. Tujuan penelitian dan pengembangan ini adalah variasi latihan kekuatan dengan whole body menggunakan ebook dengan video analisis. Metode penelitian ini menggunakan penelitian dan pengembangan (R dan D). Instrumen yang digunakan dalam mengumpulkan data yaitu memberikan angket untuk analisis kebutuhan, evaluasi para ahli dan untuk uji coba produk. Teknik analisis data menggunakan teknik analisis deskriptif kualitatif dan kuantitatif. Hasil penelitian menunjukan bahwa pengembangan variasi latihan kekuatan dengan whole body menggunakan ebook dengan video analisis dapat diterapkan dengan tingkat validitas 87,8 persen termasuk dalam kategori sangat valid. Kesimpulan dari penelitian dan pengembangan ini adalah berupa variasi latihan kekuatan whole body yang dikemas ke dalam ebook dengan video analisis dapat diterima pada atlet renang Persatuan Renang Seluruh Indonesia (PRSI) Malang.