bluuebunny/crossref_metadata_2025_split huggingface dataset:
Promoter‐pervasive transcription causes RNA polymerase II pausing to boost DOG1 expression in response to salt
Authors: Montez Miguel , Majchrowska Maria , Krzyszton Michal , Bokota Grzegorz , Sacharowski Sebastian , Wrona Magdalena , Yatusevich Ruslan , Massana Ferran , Plewczynski Dariusz , Swiezewski Szymon 
Published: January 2023

AbstractEukaryotic genomes are pervasively transcribed by RNA polymerase II. Yet, the molecular and biological implications of such a phenomenon are still largely puzzling. Here, we describe noncoding RNA transcription upstream of the Arabidopsis thaliana DOG1 gene, which governs salt stress responses and is a key regulator of seed dormancy. We find that expression of the DOG1 gene is induced by salt stress, thereby causing a delay in seed germination. We uncover extensive transcriptional activity on the promoter of the DOG1 gene, which produces a variety of lncRNAs. These lncRNAs, named PUPPIES, are co‐directionally transcribed and extend into the DOG1 coding region. We show that PUPPIES RNAs respond to salt stress and boost DOG1 expression, resulting in delayed germination. This positive role of pervasive PUPPIES transcription on DOG1 gene expression is associated with augmented pausing of RNA polymerase II, slower transcription and higher transcriptional burst size. These findings highlight the positive role of upstream co‐directional transcription in controlling transcriptional dynamics of downstream genes.
STING agonism turns human T cells into interferon‐producing cells but impedes their functionality
Authors: Kuhl Niklas , Linder Andreas , Philipp Nora , Nixdorf Daniel , Fischer Hannah , Veth Simon , Kuut Gunnar , Xu Teng Teng , Theurich Sebastian , Carell Thomas , Subklewe Marion , Hornung Veit 
Published: January 2023

AbstractThe cGAS‐STING (cyclic GMP‐AMP synthase‐stimulator of interferon genes) axis is the predominant DNA sensing system in cells of the innate immune system. However, human T cells also express high levels of STING, while its role and physiological trigger remain largely unknown. Here, we show that the cGAS‐STING pathway is indeed functional in human primary T cells. In the presence of a TCR‐engaging signal, both cGAS and STING activation switches T cells into type I interferon‐producing cells. However, T cell function is severely compromised following STING activation, as evidenced by increased cell death, decreased proliferation, and impaired metabolism. Interestingly, these different phenotypes bifurcate at the level of STING. While antiviral immunity and cell death require the transcription factor interferon regulatory factor 3 (IRF3), decreased proliferation is mediated by STING independently of IRF3. In summary, we demonstrate that human T cells possess a functional cGAS‐STING signaling pathway that can contribute to antiviral immunity. However, regardless of its potential antiviral role, the activation of the cGAS‐STING pathway negatively affects T cell function at multiple levels. Taken together, these results could help inform the future development of cGAS‐STING‐targeted immunotherapies.
The Peculiarities of Students’ Subjective Social Well-being Puring the covid-19 Pandemic Depending on Their University Attendance Mode
Authors: Baranauskienė Ingrida , Kovalenko Alla , Hryshchuk Eliso , Rohal Nina 
Published: January 2023

The study examined the peculiarities of the subjective social and psychological well-being of general civil and military students during the Covid-19 pandemic, depending on the applied attendance modes: distance or face-to-face. General civil students had higher sleep quality, but they had less social contacts, compared to military students.
The integral indicator of subjective social well-being was average in general civil and military students, and the differences were determined only for “social approval” scale, whose value was significantly higher in general civil students.
The heaviest barrier in interpersonal communication for general civil and military students was inadequate expression of emotions; and inflexibility and vagueness of emotions were the least pronounced.
Factors important for general civil students’ psychological well-being were social approval, purposefulness in their aspirations and emotional matching. At the same time, three factors were determined for military students: perceived independence, social reassurance and emotional control during interactions.
Assessment of Documented Vaccination of Adolescent Schoolchildren in Various Cities of Russian Federation
Authors: Namazova-Baranova Leyla S. , Fedoseenko Marina V. , Shakhtatinskaya Firuza Ch. , Efendieva Kamilla E. , Kaytukova Elena V. , Vishneva Elena A. , Kaliuzhnaia Tatiana A. , Tolstova Svetlana V. , Soloshenko Margarita A. , Selvyan Arevaluis M. , Leonova Elizaveta V. , Timoshkova Snezhana D. 
Published: January 2023

Epidemiological surveillance of preventive vaccinations implementation is the most crucial component in the immunoprophylaxis organization. Assessment of documented vaccination coverage indicators allows to determine the quality of routine preventive vaccination and indirectly evaluate the possible state of population immunity to vaccine preventable diseases. Continuous quality control of routine vaccination, therefore, is a component of the system for epidemic management of infectious diseases. Specific decisions should be based on its results to improve preventive vaccination quality.Сomparative analysis of the vaccination history in adolescents (studying in schools in large cities of different federal districts of Russian Federation) and recommended national immunisation schedule allowed to identify widespread systemic mistakes of vaccination status. These issues led to the uprise and spread of vaccine preventable diseases. The study results confirmed the topicality of awareness-raising activities among medical staff working on preventive vaccination. Moreover, long-standing need of vaccination schemes correction is also important through development of medical technology aimed on improvement of catch-up vaccination approach.
Curvas de respuesta fotosintética a la luz: elucidando la capacidad fotosintética de plantas de cacao (Theobroma cacao L.) aclimatadas a plena luz en Cundinamarca, Colombia
Authors: Osorio Zambrano Mayra Andreina , Rodríguez Pérez Loyla , Sierra Ríos Rafael , Terán Wilson 
Published: January 2023

Antecedentes: El cacao es un cultivo del neotrópico de importancia económica mundial, de uso ancestral en las culturas precolombinas. Se considera una especie de sombra, aunque algunos genotipos híbridos pueden crecer a plena exposición solar. Esta capacidad de tolerar diferentes niveles de luz varía, pudiendo aprovecharse para optimizar el rendimiento de los cultivos.
Preguntas: ¿Existen diferencias en la capacidad fotosintética de los clones de cacao ICS60, TSH565, FEAR5, CCN51 y EET8 frente a diferentes niveles de densidad de flujo fotónico (DFF)?
Especie de estudio: Theobroma cacao L.
Sitio y años de estudio: Estación Bambusa. Pacho, Cundinamarca, Colombia. Octubre 2021.
Métodos: Se hicieron curvas de respuesta fotosintética a la DFF en plantas de dos años de edad que crecieron expuestas a la luz solar. Se evaluó el funcionamiento del fotosistema II (PSII) a partir de las variables de la fluorescencia de la clorofila.
Resultados: Se encontraron diferencias significativas en la capacidad fotosintética de los clones, siendo CCN51 el que presentó la mayor capacidad fotosintética a mayor cantidad de DFF, mientras que el clon TSH565 mostró mejor capacidad fotosintética a baja DFF. La actividad fotoquímica fue similar entre clones, sin evidencia de afectación al PSII, sugiriendo ausencia de fotoinhibición bajo las condiciones evaluadas.
Conclusión: Estos resultados podrían guiar y recomendar el uso y aprovechamiento de estos clones bajo condiciones específicas de luz. El conocimiento generado contribuye a la implementación de prácticas de manejo que optimicen el uso de la DFF disponible para aumentar las tasas fotosintéticas, y conjuntamente, impactar en mayores rendimientos.
Socio-Philosophical Views of L.N. Tolstoy on the Institutions of Law and the State
Authors: Kerimov Alexander D. 
Published: January 2023

The author analyzes and undoubtedly appreciates the views of Leo Tolstoy concerning the essence, content, organization, and functioning of such relevant socio-cultural and political institutions as law and the state. He shows a positive attitude toward the corresponding views of a notable Russian thinker.
This article describes the specific, inalienable (in other words, obligatory in its kind and at the same time amazing) qualities and immanent properties of those writers and scientists who have succeeded in achieving excellent results in the spiritual comprehension of the world.
The fundamental conclusion is substantiated by the understanding of Leo Tolstoy of these institutions (at least in the part where it is a question of their criticism), which almost coincides with the Marxist doctrine.
Maximum Tolerable Intake of Mangrove Oyster (Crassostrea rhizophorae) from the Tapak River, Semarang City, Indonesia, which Contains Cd and Pb Metals
Authors: Haeruddin Haeruddin , Ayuningrum Diah , Oktaviani Jessika 
Published: January 2023

Mangrove oysters are a type of oyster typical for human consumption in Semarang City. The Tapak River in Semarang is a popular fishing ground for mangrove oysters, but it is also contaminated with cadmium (Cd) and lead (Pb) metals. This research was determined to analyze the concentration of Cd and Pb metals in mangrove oyster meat from the Tapak River and determine the maximum tolerable intake for humans. Oysters were collected from 3 stations with 3 replicates per station. Metal concentrations in mangrove oyster meat were analyzed with a wet digestion method followed by atomic absorption spectrophotometry. Most of the meat samples contained Cd exceeding standard concentrations for shellfish and all meat samples contained Pb exceeding the standard concentrations for shellfish. The maximum tolerable intake of Cd and Pb for human adults is 0.954 ± 0.431 mg week-1 and 0.164 ± 0.014 mg week-1 respectively.
Nutritional Characteristics of Curry fish Sea Cucumber (Stichopus vastus) using Chemicals and Physical Treatment
Authors: Sukmiwati Mery , Sumarto Sumarto , Sidauruk Santhy Wisuda , Ibrahim Irvan 
Published: January 2023

Indonesia is one of the main sources of sea cucumber in the world with high total production for domestic consumption and export market. Sea cucumber has potential as a source of traditional foods, medicine, and functional food due to the high nutritional component in sea cucumbers. The purpose of this research was to determine the different nutritional characteristics of sea cucumber obtained by chemical (ethanol extractions of 20%, 25%, and 30%) and physical treatment (boiling and steaming). The results showed that chemical treatment (30% ethanol) had the best nutritional components being composed of protein 77.35%; moisture 9.14%; ash 6.67%; fat 0.10%; and carbohydrate 6.18%, while in physical treatment, steaming had the best nutritional components in composed of 61.49% protein content, 11.53% moisture, 10.76% ash, 2.22% fat, and 14.00% carbohydrate. Amino acid profiles in chemical treatments (ethanol 20%, 25%, and 30%) were 53.60%, 56.74%, and 61.65% respectively, while the amino acid profiles in physical treatments (boiling and steaming) were 50.31% and 52.96%, respectively. Glycine and glutamate acid, non-essential amino acid, were dominated by both chemical and physical treatments.
Regional Trends of COVID-19-Like Illness- Related Emergency Department Visits in North Carolina (March 1, 2020–November 30, 2020)
Authors: Kearney Gregory D. , Hylock Ray , Park Yoo Min , Jones Katherine , Wall Bennett , Howard Rob , Iyer Pooja , Clay Maria , Endres-Dighe Stacy , Stoner Marie C.D. , Li Lei , Cajka James , Rhea Sarah 
Published: January 2023

Background An evaluation of emergency department (ED) visits and the number of patients seeking care for COVID-19-like illness (CLI) during the initial phases of the SARS-CoV-2 (COVID-19) pandemic in North Carolina has not been exclusively described. Purpose To characterize CLI-related ED visits across North Carolina from March 1 to November 30, 2020. Methods This was a retrospective, descriptive study. Data from the North Carolina Disease Event Tracking and Epidemiologic Collection Tool (NC DETECT) and the US Census Bureau were used to calculate CLI-related ED visit rates for the North Carolina resident patient population, and to compare and describe regional trends (Eastern, Piedmont, and Western). Results A total of 133,193 CLI-related ED visits were evaluated. Across the 3 regions, CLI-related ED visits followed similar trends with the highest peaks being reported in mid-July and late November 2020. The Piedmont region experienced the highest percent (56.3%), while people aged 25–49 years accounted for the largest age group (35.0 %) of CLI-related ED visits. More CLI-related ED visits occurred for White individuals (47.8%), but the Eastern region had a far higher percent (44.8%) of reported CLI-related ED visits for Black and American Indian individuals compared to the rest of the state. Limitations ICD-10-CM codes were not available during the early weeks of the pandemic, which limited the ability to evaluate CLI-related data during this time. Discussion This evaluation summarizes regional trends of CLI-related ED visits across North Carolina during the first 9 months of the COVID-19 pandemic. These results provide useful information and insight for public health officials, health care administrators, and policymakers.
Evaluating a Health Care Pathway Internship Program for Minority High School Students
Authors: Hallum Jacquelyn , Denslow Sheri , Kovalskiy Aleksandr , Poulton Ginger , Bemis Rachel , Wallace Elisabeth , Fromewick Jill 
Published: January 2023

Background In 2005, Mountain Area Health Education Center (MAHEC) collaborated with community partners to establish the Minority Medical Mentoring Program (MMMP), a semester-long health care pathway internship for high school seniors of color. This evaluation aimed to assess program participants’ perceptions of program components, identify areas for improvement, and broaden the evidence base of pathway programs. Methods Seventy-three MMMP alumni were invited to participate in an online survey. Closed- and open-ended questions aimed to assess respondents’ perceptions of program components and MMMP’s impact on their personal and professional lives. Results Forty-six alumni responded to the survey. The MMMP was perceived as universally valuable. Clinical shadowing, mentors of color, and exposure to a variety of health professionals were viewed as the most valuable program components. The MMMP strengthened participants’ awareness of health disciplines, improved their self-confidence, and increased their professional skill sets. Surprisingly, the MMMP enabled some participants to recognize health career interests outside of clinical care. Limitations Selection, social desirability, and recall bias may limit interpretation of findings. Conclusion The MMMP is highly valued by participants. A high majority of respondents (40/46; 87%) plan to have a health career in the future. Pathway programs can shape career paths, increase self-awareness, and build self-confidence for success. It is important to note that systemic racism and discrimination must be addressed to fully ensure recruitment and retention of health care providers of color.
Development of UV-Visible spectrophotometric method for the estimation of vildagliptin in different medium
Authors: Mane Sheetal Sheetal , Khan Masheer Ahmed 
Published: January 2023

A simple, accurate, precise, cost effective, rapid and sensitive UV/visible spectrophotometric method was developed for the determination of Vildagliptin in active pharmaceutical dosage form. The developed method was validated as per ICH guidelines.The purity of Vildagliptin was characterized by melting point, Fourier Transform Infra-Red and DSC. The drug was analyzed using UV/visible spectrophotometric method was validated in terms of linearity and range. The solvents used was water, 0.1 N HCl and phosphate buffer pH 7.4 and the wavelength corresponding to maximum absorbance of the drug were found at 210 nm.Melting point of drug was found 151.67°C nearly corresponds to its actual melting range. The linear response for concentration range of 2-12 µg/ml of vildagliptin for water, 0.1 N HCl and phosphate buffer pH 7.4 was recorded each with regression coefficient R = 0.9998, 0.9994 and 0.9991 respectively.The drug was confirmed by interpretation of UV spectra. Hence, proposed method stands out validated and shows a linear relationship and thus may be used for routine analysis of Vildagliptin in pharmaceutical dosage forms.
Case report: A case of Pseudoepitheliomatous hyperplasia of vulva, a differential diagnosis for squamous cell carcinoma of external genitalia in women
Authors: Harshini V , Alva Kishore 
Published: January 2023

A woman presenting with vulval lesions is to be examined and evaluated further before giving her the definitive diagnosis and proposing treatment. As the differential diagnosis may vary from benign lesions like lichen sclerosus, vulval intraepithelial neoplasia or malignant lesions like squamous cell carcinoma. We are presenting a case report wherein 70 year woman presented with features similar to squamous cell carcinoma but was diagnosed as pseudoepitheliomatous hyperplasia of vulva which is a benign lesion.Pseudoepitheliomatous hyperplasia (PEH) is a benign condition, characterized by hyperplasia of the epidermis and adnexal epithelium, closely similar to squamous cell carcinoma. PEH may be present in a number of conditions characterized by prolonged inﬂammation and/or chronic infection, as well as in association with many cutaneous neoplasms.
NATURALISASI FILSAFAT ISLAM DALAM PEMIKIRAN AL-GHAZALI
Authors: Assyabani Ridhatullah 
Published: January 2023

The thought of al-Ghazali as outlined in Tahafut al-Falasifah has been a controversy for centuries and even today it has become a debate mainly related to the view of al-Ghazali who deconstructed the building of philosophy in Islam resulting in old wounds. In the end, various negative stigmas directed at al-Ghazali. However, whether the work of al-Ghazali, as many have suspected, has resulted in haunting in philosophy or just the opposite? By re-reading al-Ghazali as a traditionalist scholar and reviewing the general opinion which says that Tahafut al-Falasifah is a representation of the conflict between philosophy and dogma; or between orthodoxy and heterodoxy, this paper will show that al-Ghazali has an important role in naturalizing science and philosophy in the frame of Islamic theology.Pemikiran al-Ghazali yang tertuang pada Tahafut al-Falasifah menjadi kontroversi selama berabad-abad, bahkan hingga saat ini menjadi perdebatan terutama terkait dengan pandangan al-Ghazali yang mendekonstruksi bangunan filsafat dalam Islam mengakibatkan luka lama. Pada akhirnya berbagai macam stigma negatif yang ditujukan kepada al-Ghazali. Namun, apakah karya al-Ghazali tersebut seperti banyak yang diduga, telah mengakibatkan kehancuran dalam fisafat atau justru sebaliknya? Dengan melakukan pembacaan ulang terhadap al-Ghazali sebagai ulama tradisionalis dan meninjau pendapat umum yang mengatakan bahwa Tahafut al-Falasifah merupakan representasi konflik antara filsafat dan dogma; atau antara ortodoksi dan heterodoksi, tulisan ini akan memperlihatkan bahwa al-Ghazali memiliki peran penting dalam melakukan naturalisasi filsafat dalam bingkai teologi Islam.
PERAN KEARIFAN LOKAL DALAM UPAYA DERADIKALISASI FAHAM RADIKAL DI KALIMANTAN SELATAN
Authors: Syaugi Syaugi , Badrian Badrian , Mubarak Faisal 
Published: January 2023

This article study discusses a portrait of religious insights among students and teachers in high schools in South Kalimantan. However, the conducted research is based on the background that the spread of radical ideology can be channeled through education, in which high school students are targeted by radical ideologies. This study intends to answer the question of how religious understanding and the role of local wisdom may encounter radicalism among Islamic education students and teachers. This article study finds that: Firstly, there are three categorizations can be mapped related to religious ideas emerging, namely: (1) Respondents whose militancy and radicalism narratives tend to dominate; (2) Some respondents also have moderation narration that dominates; and (3) some others have an equal narration of militancy and radicalism and moderation. Secondly: the locality of recitation through religious institutions of the majelis taklim with the content of Sufism-based recitation has a significant role for the students and teachers in responding to each religious issue based on moderation narration.Riset ini membahas potret faham keagamaan di dunia pendidikan dengan fokus pada siswa dan guru di Sekolah Menengah di Kalimantan Selatan. Hal ini dilatar belakangi bahwa penyebaran idiologi radikal dapat melalui jalur pendidikan, dimana siswa/siswi sekolah menengah atas dijadikan sasaran penanaman idiologi radikal. Penelitian ini untuk menjawab pertanyaan bagaimana pemahaman agama di kalangan siswa dan guru pendidikan Agama Islam? Bagaimana peran kearifan lokal dalam menangkal radikalisme di kalangan siswa dan guru pendidikan Agama Islam?. Penelitian ini menemukan bahwa: Pertama, ada tiga katagorisasi yang bisa dipetakan terkait narasi faham keagamaan yang muncul, yaitu: (1) Ada responden yang narasi militansi dan radikalisme cenderung mendominasi; (2) Ada responden yang narasi moderasinya mendominasi; (3) Ada yang antara militansi dan radikalisme di satu sisi dan moderasi di sisi lain berimbang (equal). Kedua: Lokalitas pengajian melalui lembaga keagamaan majlis taklim dengan konten pada pengajian berbasis tasawuf memiliki peran yang signifikan bagi siswa dan guru dalam merespons setiap isu keagamaan dengan basis narasi moderasi.