bluuebunny/crossref_metadata_2025_split huggingface dataset:
Evaluation of genetic diversity among some superior Cucumber (Cucumis sativus) by using Morphological and Pomological characteristics in the greenhouse under warm condition
Authors: Salmani Karim Arab , Hakimi Yousef 
Published: May 2023

AbstractGenetic variability in a crop population is essential for successful plant breeding. Fifteen cucumber (Cucumis sativusL.) genotypes were evaluated in the greenhouse under the warm condition to estimate the magnitude of their genetic variability and heritability as also morphological and pomological characteristics for three years. Genotypes were also classified into five groups based on the performance and determination of the highest discriminating trait that accounted for more significant variability using cluster analysis and principal component analysis (PCA). The measured characteristics were cucurbit chlorotic yellows virus and fungal severity, off-type plant, vine, internode, leaf, petiole, fruit and fruit trail length, leaf width, male flower percentage, fruit color and groove, fruit diameter, fruit fresh weight, fruit dry matter and ash percentage, fruit pH, EC and TSS, fruit yield, and total fruit number. The IR4 and IR5 genotypes showed the highest fruit yield (460.85 and 425.86 kg/plot) and number (108.72 and 84.22 fruit/plant). IR11 had the highest fruit length of 16.60 cm. High broad-sense heritability was associated with all the traits except for cucurbit chlorotic yellows virus and fungal severity, fruit pH, and total fruit yield. Cluster analysis and its comparison of means showed that IR4 and IR5 from the fifth cluster expressed the best agronomic traits and yield potentials in the warm condition. Hence, selection for any trait would favor genotypes in these clusters. The PCA involved fruit dry matter and TSS as the most discriminating trait that accounted for more significant cucumber variability, which should be considered in cucumber improvement programs.
RSL3 combined with cisplatin induces ferroptosis in cisplatin-resistant nasopharyngeal carcinoma cells
Authors: Wang Hao , Lin Wenchao , Qian Yechun , He Shifang 
Published: May 2023

AbstractBackground: To investigate whether RAS-selective lethal 3 (RSL3) combined with cisplatin can induce ferroptosis in cisplatin (DDP) resistant nasopharyngeal carcinoma (NPC) cells.Methods: The sensitivity of nasopharyngeal carcinoma cell lines HNE-1, CEN2Z, HONE-1 and cisplatin-resistant strain HNE-1/DDP to DDP and RAS was detected by CCK-8 method. After combined treatment of RSL3 and DDP, CCK-8 method was used. Changes of HNE-1/DDP cell activity, cell death and cellular reactive oxygen species (ROS) levels were detected by flow cytometry, cellular Fe2+and MDA levels were detected by Fe2+and malondialdehyde (MDA) kits, proteins linked to ferroptosis.Results: The sensitivity of HNE-1/DDP to DDP was much lower than that of normal HNE-1 cell line, and the IC50 of RAS was as high as (45.89±6.89) μmol/L. Flow cytometry results showed that when HNE-1/DDP was treated with RSL3 or DDP alone, the mortality rate did not exceed 30%, and the increase in ROS level did not exceed 15%. RSL3 combined with DDP could increase the cell death rate of HNE-1/DDP to 89.69% ± 9.48% and the ROS level to 18.72% ± 3.05%. Combined treatment raised intracellular Fe2+and MDA levels, reduced the expression of the ferroptosis-related protein glutathione peroxidase 4 (GPX4), and reversed RSL3 combination by the ferroptosis inhibitor Ferrostatin-1. DDP-induced death of cell inhibited the increase of ROS, Fe2+and MDA levels.Conclusions: RSL3 combined with DDP can induce ferroptosis in cisplatin-resistant strain HNE-1/DDP.
Data-Driven Transcriptional Markers for Classifying Escherichia coli and Staphylococcus aureus-Induced Sepsis in Adult Patients
Authors: Irani-Shemirani Mahnaz 
Published: May 2023

Abstract

Sepsis, a life-threatening condition, is mainly caused by gram-negative bacteria like Escherichia coli (E. coli) and gram-positive bacteria like Staphylococcus aureus (S. aureus). Differentiating between these types of sepsis is vital for appropriate treatment. Our study employed machine learning techniques and bioinformatics to identify a gene set that distinguishes E. coli-induced and S. aureus-induced sepsis in adult patients. Using least absolute shrinkage and selection operator and logistic regression, we identified a set of 25 genes that achieved an area under the curve (AUC) of 0.96 and 0.98 for E. coli and S. aureus sepsis, respectively. These genes exhibited an AUC of 0.75 for differentiating between the two types of sepsis. Internal validation highlighted significant expression differences in three genes (EIF1AY, APOBEC3B, and GUSBP3) between E. coli and S. aureus sepsis patients. After excluding these genes, the model achieved an AUC of 0.89 in distinguishing between the two types. Model stability was maintained through upsampling yielding perfect AUC scores of 1. Gene Ontology and pathway analysis revealed both common and distinct pathways between the pathogens. Our study identified 25-genes that differentiate E. coli and S. aureus sepsis in adults, however, further research is necessary to confirm their reliability in larger patient populations. Leveraging machine learning and bioinformatics in sepsis diagnosis and treatment can lead to improved patient outcomes.
miR-664a-5p promotes experimental membranous nephropathy progression through HIPK2/Calpain1/GSα-mediated autophagy inhibition
Authors: Shan Zhiming , Zhuang Zhenchao , Ren Peiyao , Zhao Li , Zheng Danna , Chen Wei , Jin Juan 
Published: May 2023

Abstract
[Background] We previously found that miR-664a-5p is specifically expressed in urinary exosomes of idiopathic membranous nephropathy (IMN) patients, but its function and mechanism in MN progression are unclear. [Objective] To investigate the function and mechanism of miR-664a-5p in MN. [Methods] The miR-664a-5p expression in HK-2 cells, exosomes, human podocytes and renal tissues were studied, as well as the activity and apoptosis of these cells, the binding of miR-664a-5p to HIPK2 mRNA, the levels of several relative proteins and autophagy, several relative characteristics of exosomes. The MN progression in MN mice model was also studied. [Results] Albumin increased the miR-664a-5p content and apoptosis of HK-2 cells, which was blocked by miR-664a-5p antagomir. miR-664a-5p bound to the 3’ UTR of HIPK2 mRNA and reduced its expression. miR-664a-5p antagomir restored albumin-mediated Calpain1 up-regulation, GSα shear and autophagy decline. Autophagy inhibitor CQ blocked the protective effect of miR-664a-5p antagomir, HIPK2 overexpression, and Calpain inhibitor SJA6017 on albumin-mediated injury. The miR-664a-5p level increased in exosomes from albumin-treated HK-2 cells, and it could be horizontally transported to podocytes through exosomes. In MN mice, exosomes from albumin-treated HK-2 cells promoted the pathological MN symptoms, and AAV-Anti-miR-664-5p (mouse homology miRNA) could improve them. [Conclusion] Albumin increases the miR-664a-5p level and causes changes in the HIPK2/Calpain1/GSα pathway, which leads to autophagy inhibition and apoptosis up-regulation of renal tubular epithelial cells. miR-664a-5p can horizontally enter podocytes through exosomes. Targeted inhibition of miR-664a-5p can reduce the apoptosis of renal tubule cells and podocytes, and may improve the MN progression.
Volumetric and dielectric properties in liquid phase of dimethyl ether - ethanol at (293.2 - 313.2) K and 1.00 MPa
Authors: Hoshina Taka-aki , Sato Kazunori , Okada Masaki , Tsuji Tomoya , Hiaki Toshihiko 
Published: May 2023

Abstract
Density and dielectric spectra were investigated in liquid phase of dimethyl ether (DME) – ethanol by an oscillating U-tube densimeter and a complex dielectric spectrometer under 1.00 MPa. The density was measured at 303.15 and 313.15 K, and converted to the excess molar volume. The excess molar volumes were no smaller than − 0.880 and − 0.909 cm3 mol–1 at 303.15 and 313.15 K, respectively. The mole fraction dependence can be correlated with Redlich-Kister equation, whose minimum was found around 0.58 for the mole fraction of DME. The dielectric constant and the dielectric relaxation time were evaluated form the complex dielectric spectra in the frequency range from 0.5 to 18 GHz at (293.2–313.2) K and 1.00 MPa. The dielectric constants and the relaxation time were decreased with the mole fraction of DME, and the latter tended to be around 25 ps in the range of mole fraction higher than 0.6. The logarithmic dielectric constants can be correlated with a similar function to Hildebrand equation with the volume fraction. The effective Kirkwood g-factor was evaluated at 293.2–313.2 K and 1.00 MPa. The g-factors were given by two liner functions crossed around 0.6 for the mole fraction of DME. Considering an atomic composition (C2H6O), the molecular sizes are not so different for DME and ethanol. Then, the solution structure was thought to be microscopically changed around 0.6 for the mole fraction of DME. The mole fraction will be utilized to switch the solubility for an extraction of amphipathic compounds.
Tricuspid Regurgitation Velocity/ Tricuspid Annular Plane Systolic Excursion (TRV/TAPSE) ratio as an indicator of disease severity and prognosis in patients with precapillary pulmonary hypertension.
Authors: Topyła-Putowska Weronika , Tomaszewski Michał , Wojtkowska Agnieszka , Styczeń Agnieszka , Wysokiński Andrzej 
Published: May 2023

Abstract
Background: Tricuspid annular plane systolic excursion (TAPSE) and tricuspid regurgitation velocity (TRV) are two echocardiographic parameters with prognostic value in patients with pulmonary hypertension (PH). When analyzed concurrently as TRV/TAPSE ratio, they allow the ventricular-pulmonary artery coupling (RVPAC) to be assessed. This could better predict the disease severity in patients with PH.
Objective: Our study aimed to evaluate the prognostic value of the TRV/TAPSE ratio echocardiographic parameter in adults with precapillary PH.
Methods: The study included 39 patients (74% women; average age, 63 years) with precapillary PH: pulmonary arterial hypertension (PAH), and chronic thromboembolic PH (CTEPH). The mean follow-up period was 16,6 ±13,3 months. Twelve patients (31%) died during observation time. We measured TAPSE as a surrogate of RV contractility and TRV reflecting RV afterload, while ventricular–arterial coupling was evaluated by the ratio between these two parameters (TRV/TAPSE). To assess disease progression and the patient's functional capacity, World Health Organization functional class (WHO FC) was determined. Patient physical capacity was also evaluated using the 6-minute walk test (6MWT). The analysis included values of N-terminal prohormone brain natriuretic peptide (NT-proBNP), which were taken routinely during the follow-up visit.
Results: The mean calculated TRV/TAPSE ratio was 0.26 ± 0.08 m/s/mm. On comparison of the TRV/TAPSE ratio to the disease prognostic indicators, we observed statistically significant correlation between TRV/TAPSE and the results of WHO FC, 6MWT and NT-proBNP. TRV/TAPSE ratio is thus a good predictor of mortality in PH patients (AUC, 0.781). Patients with a TRV/TAPSE ratio > 0.30 m/s/mm had a shorter survival time with log-rank test p< 0.0001. Additionally, ROC analysis revealed higher AUC for TRV/TAPSE than for TAPSE and TRV alone.
Conclusions: TRV/TAPSE is a promising practicable echocardiographic parameter reflecting RVPAC. Moreover, TRV/TAPSE could be viable risk stratification parameter and could have prognostic value in patients with PH.
Development Trends of Immune Activation during HIV Infection in Recent Three Decades: A Bibliometric Analysis based on CiteSpace
Authors: Gong Kang , Lai Yu 
Published: May 2023

Abstract
This study aimed to evaluate and pinpoint the status, hot areas, and frontiers of immune activation during HIV infection utilizing CiteSpace. From 1990 to 2022, we searched for studies on immune activation during HIV infection in the Web of Science Core Collection. CiteSpace was used to visually analyze the publications to identify the research status and pertinent research hotspots and frontiers in terms of the countries, institutions, authors, references, journals, and keywords. The Web of Science Core Collection yielded 5321 articles on immune activation during HIV infection. With 2854 and 364 articles, the United States and the University of California, San Francisco were the leading nation and institution in this domain. Steven G. Deeks has published 95 papers and is the most published author. The top cited articles on microbial translocation as a significant factor during HIV infection were published by Brenchley et al. Research on molecular/biology/genetics is often referenced in publications in the journals of molecular/biology/immunology. Inflammation, risk, mortality, cardiovascular disease, persistence, and biomarkers will be high-frequency words that are hot topics of research. According to the results, there was a strong collaboration between countries and organizations but little collaboration among authors. Molecular biology, immunology, and medicine are the main study subjects. The current hot topics in research are inflammation, risk, mortality, cardiovascular disease, persistence, and biomarkers. Future studies should concentrate on reducing the pathological changes caused by inflammation and altering the mechanisms of immune activation to reduce the size of the viral reservoir.
The Impact of Satellite Communications on Environmental Hazard Control: Tool for the Realization of AU Agenda 2063 Aspirations
Authors: Nkordeh Nsikan , Awomoyi Morayo 
Published: May 2023

Abstract
Africa is a developing economy and as such, emphasis has been placed on the achievement of revolutionary goals that will place her on a similar rank as the developed economies. Pertaining to this objective, Heads of States and government all over Africa instigated the African Union (AU) Agenda 2063, which is a framework put in place to achieve a continental transformation over the next 40 years. The use of satellites has been proven a major influencer of economic growth since it facilitates the exchange of information. Environmental hazards such as climate changes, pollution, and inefficient waste management can be classified as one of the drawbacks to achieving this economic growth we hope to accomplish. The purpose of this paper is to analyze and examine satellite communication as a tool for the attainment of an integrated, prosperous and peaceful Africa by means of combatting environmental hazards in the continent.
Observed Heatwaves Characteristics and Variability over Saudi Arabia
Authors: Syed F.S. , Al-Azemi M.A. , Zamreeq A. , Islam M. Nazrul , Ghulam A. 
Published: May 2023

Abstract
Heatwaves are prolonged periods of excessively hot weather, which can have significant impacts on human health, agriculture, and the environment. Climate change has been linked to an increase in the frequency, intensity, and duration of heatwaves. As the global average temperature rises, heatwaves are becoming more common and more severe. The Arabian Peninsula is warming at a faster rate as compared to the globe in the recent decades. In this paper the mild, moderate, severe, and extreme heatwaves defined by 85th, 90th, 95th and 99th percentile respectively are analyzed over Saudi Arabia using historical daily maximum and minimum temperature observations for the period 1985–2021. The large number of mild heatwaves are observed all over Saudi Arabia while extreme heatwaves are dominant in the northwestern region. Moderate and severe heatwaves are observed less in both the Red Sea and Arabian Gulf coastal regions. The heatwaves are intense in the northern and central areas as compared to other regions of the country. Heatwaves frequency, intensity and length in Saudi Arabia are in increasing trends, along with the increase in the heatwaves season length. The heatwaves frequency and intensity are observed large during the ENSO La Nina and neutral phases along with NAO negative phase as well as IOD negative and neutral phases. However, further investigation is required to see the occurrence of heatwaves in different climate zones over Saudi Arabia at various seasons and their teleconnection to largescale circulations.
Internal Migration and the Effective Price of State and Local Taxes
Authors: Drukker Austin J. 
Published: May 2023

Abstract
This paper examines the mobility response of high-income households in the United States to a provision in a 2017 tax law that limited the federal deductibility of state and local taxes. The increase in the effective price of state and local taxes induced by the cap on deductibility caused high-income households to leave high-tax states in favor of low-tax states and to prefer low-tax states to high-tax states conditional on moving. The findings suggest that policymakers should take seriously the prospect that high-income taxpayers may flee states that tax them heavily, which could have long-lasting implications for states’ fiscal positions.
JEL Codes: H24, H31, H71, H73
A Novel Six-Wing Chaotic System with Line Equilibrium and its Application in Image Encryption
Authors: Li Ping , Xia Lei , Fan Yigang , Qian Jin 
Published: May 2023

Abstract

This brief introduces a novel chaotic system with line equilibrium, which has six wings. The dynamic properties of the system, including chaos, period-doubling bifurcation, and coexisting attractors have been verified by analysis tools such as phase diagram, bifurcation diagram, Poincare map and Lyapunov exponents (LEs). Different kinds of attractors are obtained by controlling one parameter. Especially, offset boosting control and sensitivity to initial values are discovered. In addition, two positive LEs are observed when a parameter is fixed. Emulated by Multisim, the consistency of analog circuit and numerical simulation confirms the feasibility of the theoretical model of the new system. The proposed system is applied to image encryption based on classical algorithm, it is histogram, adjacent pixel correlation, attack resistance, and information entropy can be verified, and the experimental results show that the new system is very sensitive to key and plaintext and can effectively resist statistical and differential attacks.
Species identification method by a new non-invasive technique in Korean endangered terrestrial snail, Koreanohadra koreana (Castropoda: Mollusca)
Authors: Cha Deokjea , Kim Jin-Young , Kim Kang-San , Kim Young-Joong 
Published: May 2023

Abstract
Koreanohadra koreana (K. koreana) is an endemic species in South Korea that is listed as endangered. While the ecology and phylogenetics of K. koreana have been studied, its morphological similarity to the related species Koreanohadra kurodana (K. kurodana), can make species identification difficult. Furthermore, this has led to confusion when determining essential habitat information for the conservation of K. koreana. To bypass this issue, we have developed a noninvasive species identification method that can genetically differentiate between them. While there are already various noninvasive genomic DNA (gDNA) extraction methods that utilize the mucus from mollusks, they are limited as they require the target species to be physically located. To address this, in this investigation a method of extracting gDNA from the feces of snails was developed. The method utilized a primer set to amplify a cytochrome b fragment from K. koreana but not K. kurodana or other terrestrial snails. The feces of terrestrial snails could thus be used to obtain gDNA to a genetically usable level if collected within 5 days of excretion. This noninvasive species identification method using feces will help to facilitate genetic research without harming the endangered species and if the target species is not physically in the habitat. Moreover, K. koreana and K. kurodana could perhaps be further distinguished, using their habitat information to help facilitate essential conservation measures.
Influence of Preoperative Pro-Inflammatory Cytokines and Nutritional Status on Skin Scar Hyperplasia
Authors: 湘 魏 , 张 一明 , 卢 杨 , 张 明石 , 盛 薛新 , 周 紫飞 , 徐 元志 
Published: May 2023

Abstract
Skin scar hyperplasia is a common complication following surgical procedures. We aimed to investigate certain sensitive indicators of hypertrophic scars formation and search potential strategies for reducing scar formation. Therefore, our objective was to collecte nutritional status, blood samples, and scar tissue samples from 123 patients undergoing internal fixation surgery between 2019 and 2022. Patients were classified into the hypertrophic scar (HS) group (n=33) and the non-hypertrophic scar (NHS) group (n=90) based on the degree of skin scar hyperplasia one year after surgery. Preoperative inflammatory factor (CRP, white blood cell, lymphocyte%, neutrophil%, monocyte%, IL-5, IL-6, IL-12p70, INF-γ) levels and nutritional status (ideal body weight (%), weight loss, lymphocyte, blood albumin, transferrin, pre-albumin) were measured by the blood test. RNA sequencing was used to test the tissue samples to explore related signaling pathways involving hypertrophic scars formation. In this study, we demonstrated the higher pro-inflammatory cytokine levels (CRP, neutrophil%, monocyte%, and IL-12p70)and worse nutritional status（ideal body weight, Lymphocyte, and Blood Albumin） occur in the HS group, with statistically significant difference (p < 0.05). In addition, Differential gene analysis found higher gene expression levels of inflammatory response, cell proliferation, and angiogenesis pathways in the HS group. Elevated preoperative inflammatory levels and poor nutritional status have significant effects on skin scar formation after surgery due to the role of pro-inflammatory cytokines in scar formation. Therefore, improving nutritional levels and controlling preoperative inflammation might be potential strategies for reducing scar formation.
Trial registration number: ChiCTR2300069087
Date of registration: 2023-03-06
Prediction and interpretation of gamma pass rate based on SHAP value feature selection
Authors: Ni Qianxi , Chen Luqiao , Zhu Jun , Pang Jinmeng , Wang Zhiyan , Yang Xiaohua 
Published: May 2023

Abstract
Background SHAP values are suggested as a unique measure of feature importance in machine learning prediction models. It can explain the output of any machine learning prediction model and can also participate in the construction of machine learning prediction models as a feature selection mechanism for handling high-dimensional data. In this study ,the SHAP values and extreme gradient boosting(XGBoost) algorithm were combined to select the best radiomics features for the establishment of the gamma pass rate(GPR) prediction model.The feasibility and effectiveness of the prediction model were evaluated .
Methods Retrospective analysis of the 3D dosimetric verification results based on measurements with GPR criteria of 3%/2 mm and 10% dose threshold of 196 pelvic intensity-modulated radiation therapy (IMRT) was carried. Radiomic features were extracted from the dose files, from which the XGBoost algorithm based on SHAP values was used to select the optimal feature subset as the input for the prediction model. Four machine learning classification models were constructed when the number of features was 50, 80, 110 and 140 respectively, and the AUC values, recall and F1 scores were calculated to assess the classification performance of the prediction models.
Results The prediction model constructed based on the 110 features selected by SHAP values had an AUC value of 0.81, a recall of 0.93 and an F1 score of 0.82, which were better than the other three models.
Conclusion It is feasible to use the SHAP values in combination with the XGBoost algorithm to select the best subset of radiomic features for the GPR prediction models. The global explanations and single-sample explanations of the model output through SHAP values may offer reference for medical physicists to provide high-quality plans, promoting the clinical application and implementation of GPR prediction models, and providing safe and efficient personalized QA management for patients.