bluuebunny/crossref_metadata_2025_split huggingface dataset:
Transcriptomic analysis of ipsilateral spinal cord in rats after bone fracture
Authors: Wang Shen , Deng Jin , Yuan Shaoxun , Lu Qingguo , Gu Xinyi , Huang Chen , Guo Shuhang , Yu Dehong , Yin Xiaofeng 
Published: May 2023

Abstract
Backgroud: A large amount of research has shown that spinal cord injury causes bone loss and increases fracture risk, while spinal cord injuries caused by fractures and their underlying molecular mechanisms still need further investigation.Methods To investigate the specific changes in the spinal cord after bone fractures, we obtained L4-L5 spinal cord segments from the same side of SD rats with tibial fractures at 0, 3, 7, 14, and 28 days after the fracture. Gene Ontology (GO) enrichment analysis, KEGG pathway analysis, and Ingenuity Pathway Analysis(IPA) were used to analyze the differential gene expression of the genes at different time points .Results Our sequencing results showed that the transcriptional changes in the spinal cord after fracture developed towards the direction of restoring normal physiological function. At 3 and 7 days after fracture, the same-side L4-L5 spinal cord segment showed significant activation of neurodegeneration and central nervous system development, with neurodegeneration activation being most significant at 3 days after fracture and the spinal cord also showing activation of cell survival regulation effects after 7 days of bone fracture. At 14 days after bone fracture, the spinal cord showed significant enrichment in the myelin sheath formation signaling pathway, activation of cell death in the central nervous system, GO terms relating to synaptic function were also significantly enriched. The spinal cord had active gene transcription, protein synthesis, and degradation metabolism processes at these three time points after fracture. At 28 days after fracture, the spinal cord showed recovery of motor function. In addition, a series of important genes in the spinal cord after fracture were differentially expressed, including PCP4 and Krit1. Rictor, TEAD1, torin1, DDX5, CAB39L, and Ngf in the spinal cord may act as upstream master regulators to play a role in fracture repair.Conclusions We speculate that local injury stimulation of the fracture through DRG enters the intermediate neurons of the spinal cord dorsal horn, triggering a series of adaptive changes including activation of neurodegeneration and central nervous system development in the spinal cord, among other changes. With the involvement of important molecules such as PCP4 and Krit1, the physiological function of the spinal cord gradually recovers after fracture, reducing the risk of disuse osteoporosis and promoting fracture repair. This study provides an understanding of the transcriptome changes in the spinal cord following fracture at different time points, and the changes observed across these time points, and screens for important genes that might participate in the regulation of spinal cord fracture healing, providing a sequencing basis for exploring the bidirectional relationship between fracture and the spinal cord.
Phonological Awareness Mediates the Relationship between DCDC2 and Reading Performance with the Influence of Home Environment
Authors: Li Miao , DeMille Mellissa , Lovett Maureen , Bosson-Heenan Joan , Frijters Jan , Gruen Jeffrey 
Published: May 2023

Abstract

Proficient reading requires critical phonological processing skill that interact with both genetic and environmental factors. However, the precise nature of the relationships between phonological processing and genetic and environmental factors are poorly understood. We analyzed data from the Genes, Reading and Dyslexia (GRaD) Study on 1,419 children ages 8 to 14 years from African-American and Hispanic-American family backgrounds living in North America. The analyses showed that phonological awareness mediated the relationship between DCDC2-READ1 and reading outcomes when parental education and socioeconomic status was low. The association between READ1 and reading performance is complex, whereby mediation by phonological awareness was significantly moderated by both parental education and socioeconomic status. These results show the importance of home environment and phonological skills when determining associations between READ1 and reading outcomes. This will be an important consideration in the development of genetic screening for risk of reading disability.
Explore the possible pathway of improving liver and heart injury in diabetes nephropathy based on bioinformatics analysis
Authors: Yang Ye , Wang Qin , Wang Xiaobei , Wang Xinling 
Published: May 2023

Abstract
Background This study explore the possible pathway of MicroRNA-130a, TXNIP, CD44 and TGF-β1 improving liver and heart injury in diabetes nephropathy based on bioinformatics ananlysis.Methods Screening Key Genes Using Bioinformatics Analysis. The biochemical index and serum levels of MicroRNA-130a, TXNIP, CD44 and TGF-β1 were detected and analyzed by Pearson correlation analysis in 100 DN patients and 50 healthy controls. The rats model were randomly divided into two groups. The expression of MicroRNA-130a, TXNIP, CD44 and TGF-β1 in liver and heart and the morphological changes was detected.Results Screening and Analysis of Differentially Expressed Genes MicroRNA-130a and TXNIP, CD44 and TGFBI Involved in diabetes Nephropathy by Bioinformatics Methods. Compared to healthy controls, serum levels of MicroRNA-130a were decreased, while levels of TXNIP, CD44 and TGF-β1 were elevated in DN patients. Moreover, MicroRNA-130a was negatively correlated with TXNIP, CD44 and TGF-β1. In DN rats, the levels of TXNIP, CD44 and TGF-β1 in the liver and heart tissues were significantly elevated, while MicroRNA-130a levels were significantly decreased, compared to the NC group.Conclusion Upregulate MicroRNA-130a and decrease TXNIP, CD44 and TGF-β1 may participate in liver and heart injury pathway of diabetes nephropathy.
IFNα induces CCR5 in CD4+ T-cells, causing its anti- HIV inefficiency and its subsequent pathogenic elevation, partially controlled by anti-HIV therapy
Authors: Gallo Robert , Buanec Hélène , Schiavon Valérie , Mérandet Marine , Howkit Alexandre , SONG HONGSHUO , Bergerat David , C Fombellida Lopez , Bensussan Armand , Bouaziz Jean-David , Burny Arsène , Darcis Gilles , Sajadi Mohammad , Kottilil Shyamasundaran , Zagury Daniel 
Published: May 2023

Abstract
Like EC, we find that ART-treated patients control serum IFNα concentration and show few immune cell alterations enabling a healthy but fragile medical status. However, treatment interruption leads to elevated IFNα reflecting virus production indicating that like EC, ART does not achieve a virological cure. The immune system becomes overwhelmed by multiple immune cell abnormalities as found in untreated patients. These are chiefly mediated by elevated IFNα inducing signaling checkpoints abnormalities, including PD1, in cytotoxic immune cells. Importantly, during acute infection, elevated IFNα correlated with HIV load, and we found that IFNα enhances CCR5, the HIV coreceptor in CD4+ T-cells, impairing its antiviral response and accounting for the pathogenic vicious cycle: HIV → IFNα ↗ → infected CD4+ T-cells ↗ →HIV ↗. This study opens immunotherapeutic perspectives showing the need to control IFNα in order to convert ART patients into EC.
Industry applications of identifying spot laser welded nugget for automatic ESS manufacturing process
Authors: Chen Youshyang , Chang Jieh-Ren , Mohammad Ashraf , Kuo Fang-Chang 
Published: May 2023

Abstract

Recent advancements in energy storage along with power electronic technology have made battery energy storage systems (ESS) a feasible alternative for current power applications. Battery packs with lithium-ion (Li-ion) pouch cells are the main source of ESS. However, it is a big trouble that improper voltage and impedance of laser welding significantly affect the whole battery module during battery pack manufacturing stages, causing the cell imbalance inside and eventually resulting in a thermal runaway of battery pack and non-durable use. Importantly, the formation of nuggets welded can be classified as good (GD) and not-good (NG) based on the labels after peeling off the flyer of Li-ion pouch cell. Interestingly, it is usually a standard practice in this industry to include substantial numbers of redundant welds to gain confidence in the structural stability of the welded component. Thus, a non-destroyed and low-cost detection for identifying the nuggets is absolutely necessary. An effective methodology is motivated and proposed with three procedures for the identification of laser-welded nuggets. At first, the nuggets are detected and separated from a grayscale image. Image features are extracted to train the nugget images on the advanced detector model constructed to identify the GD and NG nuggets. Second, this research develops five models for achieving this purpose of detector; one is called the nugget model developed in convolution neural network (CNN) technique, and the others use the transfer learning of the most popular pre-trained models. From the comparative studies, it is found that the residual network (ResNet) model more effectively classifies the nuggets with 100% accuracy rate than that of the other listed models. Finally, this research has significant application contributions of battery manufacturing industries to produce highly efficient welded nugget products by overcoming the cost-ineffective problems of manual inspection; thus, it further helps this industry simultaneously reduce productive inspection time and increase the manufacturing efficiency of ESS at a lower cost without human intervention than the past.
Compassion Focused Therapy (CFT) for the reduction of the self-stigma of mental disorders : The COMpassion for Psychiatric disorders And Self-Stigma (COMPASS) study protocol for a randomized controlled study.
Authors: Riebel Marie , Rohmer Odile , Charles Emilie , Lefebvre François , Weibel Sébastien , Weiner Luisa 
Published: May 2023

Abstract
Background:
People with mental disorders face frequent stigmatizing attitudes and behaviors from others. Importantly, they can internalize such negative attitudes and thus self-stigmatize. Self-stigma is involved in diminished coping skills leading to social avoidance and difficulties in adhering to care. Reducing self-stigma and its emotional corollary, shame, is thus crucial to attenuate the negative outcomes associated with mental illness. Compassion Focused Therapy (CFT) is a third wave cognitive behavioral therapy that targets shame reduction and hostile self-to-self relationship and allows for symptom improvement while increasing self-compassion. Although shame is a prominent part of the concept of self-stigma, the efficacy of CFT has never been evaluated in individuals with high levels of self-stigma. The purpose of this study is to evaluate the efficacy and acceptability of a group-based CFT program on self-stigma, compared to a psychoeducation program for self-stigma (Ending Self-Stigma) and to treatment as usual (TAU). We hypothesize that diminished shame and emotional dysregulation and increased self-compassion will mediate the relationship between self-stigma improvements post-therapy in the experimental group.
Methods:
This seven-center trial will involve 336 participants diagnosed with a severe mental illness and reporting high levels of self-stigma. Participants will be randomized into one of three treatment arms: 12 week-treatment of Compassion-Focused Therapy (experimental arm), 12 week-treatment of Psychoeducation (active control arm), and TAU (treatment as usual - passive control arm). The primary outcome is the decrease of self-stigma scores on a self-report scale, i.e., ISMI, at 12 weeks.
Secondary endpoints include sustainability of self-stigma scores (ISMI) and self-reported scores regarding target psychological dimensions, e.g., shame and emotional regulation, social functioning and psychiatric symptoms. Assessments are scheduled at pretreatment, post-treatment (at 12 weeks), and at 6-month follow-up. Acceptability will be evaluated via (i) the Credibility and Expectancy Questionnaire at T0, the Consumer Satisfaction Questionnaire for Psychotherapeutic Services posttreatment and at 6-month follow-up, (iii) attendance and (iv) dropout rates.
Discussion:
This study will evaluate the potential efficacy and acceptability of a group-based CFT program on the decrease of self-stigma, and thereby contribute to the continuing development of evidence-based therapeutic interventions for the internalized stigma of mental disorders. `
Trial registration:
This trial has been registered on January 26, 2023: clinicaltrials.gov NCT05698589 https://www.clinicaltrials.gov/ct2/show/NCT05698589
Isolation of bioactive phytochemicals from Crinum asiaticum along with their cytotoxic and TRAIL-resistance abrogating prospect assessment
Authors: Rakhi Sharmin , Hara Yasumasa , Islam Md. , Manome Teruhisa , Alam Safaet , Emon Nazim , Al-Mansur Muhammad , Kuddus Md , Sarkar Md. , Ishibashi Masami , Ahmed Firoj 
Published: May 2023

Abstract
Crinum asiaticum L. (Amaryllidaceae) is a perennial bulbous herb, locally utilized for possessing multifaceted pharmacological properties including anticancer, immune-stimulating, analgesic, antiviral, antimalarial, antibacterial, and antifungal, in addition to their popularity as an aesthetic plant. Separation of MeOH extract of C. asiaticum leaves yielded three known compounds as cycloneolitsol (1), hippeastrine (2) and β-sitosterol (3). Among these, compounds 1 and 2 were subjected to the cytotoxic assay and found that 1 decreased cell viability to 45% and 8% against HCT116 cells; 15% and 9% against DU145 cells; 63% and 23% against Huh7 cells at 100 µM and 200 µM concentrations, respectively. Similarly, 2 decreased cell viability to 10% and 7% against HCT116 cells; 25% and 15% against DU145 cells; 26% and 18% against Huh7 cells at 100 µM and 200 µM concentrations, respectively. When tested for TRAIL-resistance abrogating activity, 1 (100 µM) along with TRAIL (100 ng/mL) showed moderate activity in AGS cells producing 25% more inhibition than the agent alone. Whereas (20 and 30 µM) in combination with TRAIL (100 ng/mL) exhibited strong activity in abrogating TRAIL-resistance and caused 34 and 36% more inhibition in AGS cells, respectively. The in-silico studies of compounds 1 and 2 revealed high docking hits in the TRAIL and other cancer-associated proteins which indicates a good correlation with the cell-based assay. It is still recommended to conduct further investigations to understand their exact molecular mechanism.
Pyruvate dehydrogenase complex integrates the metabolome and epigenome in CD8+ memory T cell differentiation in vitro
Authors: Tarasenko Tatiana , Banerjee Payal , Gomez-Rodriguez Julio , Gildea Derek , Zhang Suiyuan , Wolfsberg Tyra , Jenkins Lisa , Program NISC Comparative Sequencing , McGuire Peter 
Published: May 2023

Abstract
Modulation of metabolic flux through pyruvate dehydrogenase complex (PDC) plays an important role in T cell activation and differentiation. PDC sits at the transition between glycolysis and the tricarboxylic acid cycle and is a major producer of acetyl-CoA, marking it as a potential metabolic and epigenetic node. To understand the role of pyruvate dehydrogenase complex in T cell differentiation, we generated mice deficient in T cell pyruvate dehydrogenase E1A (Pdha) subunit using a CD4-cre recombinase-based strategy. Herein, we show that genetic ablation of PDC activity in T cells (TPdh-/-) leads to marked perturbations in glycolysis, the tricarboxylic acid cycle, and OXPHOS. TPdh-/- T cells became dependent upon substrate level phosphorylation via glycolysis, secondary to depressed OXPHOS. Due to the block of PDC activity, histone acetylation was also reduced, including H3K27, a critical site for CD8+ TM differentiation. Transcriptional and functional profiling revealed abnormal CD8+ TM differentiation in vitro. Collectively, our data indicate that PDC integrates the metabolome and epigenome in CD8+ memory T cell differentiation. Targeting this metabolic and epigenetic node can have widespread ramifications on cellular function.
The Impact of CFTR Modulator Triple Therapy on Type 2 Inflammatory Response in Patients with Cystic Fibrosis
Authors: Mehta Ajay , Lee Irene , Li Galvin , Jones Marieke , Hanson Lydia , Lonabaugh Kevin , List Rhonda , Borish Larry , Albon Dana 
Published: May 2023

Abstract
Background
Treatment of cystic fibrosis (CF) has been revolutionized by the use of cystic fibrosis transmembrane conductance regulator (CFTR) protein modulators such as elexacaftor/tezacaftor/ivacaftor (ETI) triple therapy. Prior studies support a role for type 2 (T2) inflammation in many people with CF (PwCF) and CF-asthma overlap syndrome (CFAOS) is considered a separate clinical entity. It is unknown whether initiation of ETI therapy impacts T2 inflammation in PwCF. We hypothesized that ETI initiation decreases T2 inflammation in PwCF.
Methods
A single center retrospective chart review was conducted for adult PwCF. As markers of T2 inflammation, absolute eosinophil count (AEC) and total immunoglobulin E (IgE) data were collected longitudinally 12 months prior to ETI therapy initiation and 12 months following therapy initiation. Multivariable analyses adjusted for the age, gender, CFTR mutation, disease severity, inhaled steroid use, and microbiological colonization.
Results
There was a statistically significant reduction (20.10%, p < 0.001) in 12-month mean IgE following ETI initiation; this change remained statistically significant in the multivariate model. The longitudinal analysis demonstrated no change in AEC following therapy initiation.
Conclusion
This study shows reduction in IgE but no change in AEC after ETI therapy initiation. We think that the lack of influence on AEC argues against an impact on previously established T2 inflammation and that the reduction in IgE is likely related to antigen load reduction post ETI. Further studies are warranted to determine the underlying mechanism of ETI impact on T2 inflammation and possible role for asthma immunomodulator therapy post ETI initiation in CFAOS.
Importance of Anemia in Heart Failure over Blood Pressure Variability
Authors: Kiuchi Shunsuke , Hisatake Shinji , Kabuki Takayuki , Dobashi Shintaro , Murakami Yoshiki , Ikeda Takanori 
Published: May 2023

Abstract
The evaluation of arteriosclerosis (vascular function) is important when treating heart failure (HF). Vascular dysfunction is associated with anemia through renal function and endothelial nitric oxide synthase, and many patients with HF have anemia. Additionally, blood pressure variability (BPV) caused by vascular dysfunction is also associated with HF prognosis. However, how anemia and BPV may affect HF prognosis is unclear. Between January 2012 and July 2018, 214 patients with HF were hospitalized, the cardio-ankle vascular index (CAVI) as an index of arteriosclerosis of whom was measured. According to the CAVI, the patients were divided into the elevated and preserved CAVI groups. Furthermore, we investigated the factors related to major adverse cardiovascular events (MACE). MACE was defined as cardiovascular death or rehospitalization within 1 year after discharge. In the elevated CAVI group, significant differences in body mass index (BMI), BPV, left ventricular dimension, and hemoglobin levels were observed between patients with MACE and those without MACE. Meanwhile, in the preserved CAVI group, significant differences in BMI, diastolic/mean blood pressure values, and hemoglobin levels were observed. The multivariate analysis showed an independent association between hemoglobin level and MACE occurrence in both the elevated and preserved CAVI groups (elevated CAVI group: hazard ratio [HR] = 0.800, P = 0.045; preserved CAVI group: HR = 0.783, P = 0.049 {model 1}, and HR = 0.752, P = 0.023 {model 2}). Anemia was independently associated with HF prognosis with or without arteriosclerosis. In HF with arteriosclerosis, BPV may also be useful for evaluating the prognosis.
Development, chlorophyll content and nutrient accumulation in in vitro shoots of Melaleuca alternifolia under light-wavelengths and benzylaminopurine (BAP)
Authors: Gonzales-Alvarado Antony Cristhian , Cardoso Jean Carlos 
Published: May 2023

Abstract
In vitro growing of the Melaleuca could contribute to the cloning of superior genotypes. The studies of factors that affect the micropropagation are necessary, especially by the use of recent technologies, such as the use of light-emitting diodes (LEDs). This study aimed at better understanding the effects of wavelengths on the development and physiology of melaleuca cultivated in vitro, as well as the interaction of LEDs with the main cytokinin used in micropropagation, the N6-Benzylaminopurine (BAP). The BAP and wavelengths, as well as the interaction between them, had a strong influence on most of the variables analyzed, altering the in vitro development, chlorophyll concentrations in the plants, as well as changing the different variables analyzed from the culture medium, such as pH, EC, quantities of Ca2+, Mg2+ and P in the culture media and the nutrient accumulation in the shoots. The results demonstrate that the main effects of the addition of BAP in the in vitro cultivation of melaleuca are: the increase in the number of shoots which resulted in greater increase in fresh and dry mass; reduction of height and chlorophyll contents; completely inhibition of adventitious rooting; higher consumption of Mg and lower consumption of Ca and P from the culture media; higher contents of Fe and lower contents of P, S, Mn, Cu and B in the in vitro tissues.
New Drug Discovery of Cardiac Anti-Arrhythmic Drugs: Insights in Animal Models
Authors: Sharma Ashish Kumar , Singh Shivam , Bhat Mehvish , Gill Kartik , Tantray Junaid , Jose Divyamol , Gupta Rashmi , Sharma Rajesh , Sahu Sanjay Kumar , Rathore Gulshan , Chandolia Priyanka , Singh Mithilesh , Mishra Anurag , Raj Shobhit , Gupta Archita , Agarwal Mohit , Gupta Anamika , Gupta Prashant , Vashist Ankit , Vaibhav Parth , Kathuria Nancy , Yadav Vipin , Singh Ravindra Pal , Garg Arun , Zaid Mohammad , Kifayat Sumaiya , Yangzom Tsering , Kumar Sachin , Shakya Anjali 
Published: May 2023

Abstract
In the normal heart cardiac rhythm is controlled by microscopic and macroscopic structures. Abnormality of pacemaker or electrical conduction aberrations causes arrhythmic disorders. Cardiac Arrhythmias may be fortunate, typical, threatening and eventually fatal. Cardio-vascular arrhythmia happens commonly in clinical practise affecting drastically to the patients on digitalis, anaesthesia and acute myocardial infarction. Both traditional and genetic animal models of arrhythmias, their characteristics, and their significance are summarized as: In Vitro Models: Isolated guinea pig papillary muscles, Action potential and refractory period in isolated guinea pig papillary muscle, Langendorff technique & Acetylcholine or potassium induced arrhythmia. In Vivo Models- Chemically induced arrhythmia: Aconitine antagonism in rats, Digoxin induced arrhythmia in rats, Strophanthin/ouabain induced arrhythmia, Adrenaline induced arrhythmia & Calcium induced arrhythmia. Electrically induced arrhythmia: Ventricular fibrillation electrical threshold, Programmed electrical stimulation induced arrhythmia & Sudden coronary death models in dogs. Exercise induced ventricular fibrillation. Mechanically induced arrhythmia: Reperfusion arrhythmia in rats, Reperfusion arrhythmia in dogs & Two stage coronary ligation in dogs. Genetic models of arrhythmias. Conclusion: Experimental models of cardiac arrhythmias, traditional and genetic gives quick summary of the most popular animal models, their characteristics, and their significance for new drug discovery of antiarrhythmic agents.