bluuebunny/crossref_metadata_2025_split huggingface dataset:
Randomized controlled trial of overall functional exercise process in perioperative of percutaneous transforaminal endoscopic discectomy
Authors: Wang Shuang , Yu Hai-Long , Zheng Liang , Ma Jun-Xiong , Wang Hong , Xiang Liang-Bi , Chen Yu 
Published: December 2022

Background:
Percutaneous transforaminal endoscopic discectomy (PTED) has become the standard surgery for the patients of lumbar disc herniation with the advantages of less trauma and rapid recovery. But still some patients have poor prognosis after PTED. A major risk factor associated with the poor prognosis may be the unfit function exercise.

Objective:
To discuss the effect of overall functional exercise process for PTED.

Methods:
In January 2019 to June 2020, a single center randomized controlled trial was proceeded. The patients scheduled for PTED were randomly divided into the experimental group, which received overall functional exercise and the control group, which received routine process. The overall process included advance, whole-course exercise and integrating of traditional Chinese medical methods. The general information, visual analog scale (VAS) score and Oswestry Dysfunction Index (ODI) score at each follow-up point perioperative period were compared between the 2 groups.

Results:
There were no significant differences in the general information, the preoperative VAS and ODI. On the 3rd day after operation, the VAS of low back pain and leg pain in the experimental group were lower than the control group. One month after operation, the VAS of low back pain in the experimental group was lower than that in the control group. One to 3 months after operation, the ODI scores of the experimental group were better than that of the control group. There was no significant difference in modified MacNab index between the experimental group and the control group.

Conclusion:
Function exercise is important for the prognosis of minimally invasive lumbar surgery. The overall function exercise process perioperative is helpful to relieve the short-term pain of the patients and significantly improve the prognosis.
Manifestation of an undifferentiated uterine sarcoma in a 51 years old patient and its prognosis: A case report
Authors: Pantelić Miloš , Gvozdenovic Ljiljana , Panjković Milana , Stojić Marko , Stajić Dragan , Petrić Aleksandra , Trajković Sonja Pop , Trenkić Milan , Simić Dušan , Živadinović Lazar , Živadinović Aleksandar 
Published: December 2022

Introduction:
Undifferentiated uterine sarcoma is a rare histological subtype of uterine sarcoma. This study aimed to summarize the clinical and pathological presentation of this case.

Case report:
A 51-years-old patient was admitted to the clinic because of severe pain in the lower abdomen, and scanty bleeding from the genitals. Gynecological examination revealed an enlarged uterus. Conventional and Doppler transvaginal sonography detected a tumorously altered uterus with a maximum diameter of 20 cm a tumefaction with unclear borders and a diameter of 10 cm, with hyperechoic and hypoechoic fields within the tumefaction, presenting pathological vascularization and reduced values of the (Pulsatile index  ≤ 1) and (Resistance index  ≤ 0.40). Preoperatively, the chest, abdomen, and pelvis were examined. The patient underwent surgery and total abdominal hysterectomy with bilateral salpingo-oophorectomy, and partial omentectomy, with complete removal of the tumor. A pathohistological diagnosis, of undifferentiated uterine sarcoma, was made by excluding other types of uterine sarcomas. At the control examination after completion of chemotherapy, recurrence was ascertained.

Conclusion:
undifferentiated uterine sarcoma is an aggressive malignant tumor that in most cases shows rapid progression of the disease after complete resection of the tumor, with a poor prognosis.
Modulating factors of fibromyalgia in women during the COVID-19 pandemic: A protocol for systematic review
Authors: Núñez-Recio Iván , García-Iglesias Juan Jesús , Martín-López Cristina , Navarro-Abal Yolanda , Fagundo-Rivera Javier , Climent-Rodríguez José Antonio , Gómez-Salgado Juan 
Published: December 2022

Background:
Fibromyalgia is a disease that affects mostly women and is related to stressors. The aim of this study was to assess how the Coronavirus disease 2019 pandemic has affected women with fibromyalgia.

Methods:
A systematic review was conducted in the PubMed, ScienceDirect, and Springer Link databases, following the recommendations of the preferred reporting items for systematic reviews and meta-analyses statement. The methodological quality was assessed using the Joanna Briggs Institute critical appraisal tools for non-randomized studies.

Results:
A total of 6 studies were included. Most results indicated a worsening in the mental health of women with fibromyalgia during the pandemic, and this in turn impacted on physical health.

Conclusion:
The mental health of women with fibromyalgia was more affected during the Coronavirus disease 2019 pandemic period than that of non-fibromyalgia sufferers. This was also reflected in the worsening of symptoms and weakness in performing daily activities.
Large gastroduodenal artery pseudoaneurysm, arterioportal fistula and portal vein stenosis in chronic pancreatitis treated using combined transarterial embolization and transportal stenting: A case report
Authors: Lim Sung Gong , Park Sung Eun , Nam In Chul , Choi Ho Cheol , Won Jung Ho , Jo Sa Hong , Baek Hye Jin , Moon Jin Il , Cho Eun , Jang Jae Yool 
Published: December 2022

Rationale:
Chronic pancreatitis is an ongoing fibroinflammatory disease of the pancreas characterized by irreversible damage to the pancreatic parenchyma and ductal system. Besides, chronic pancreatitis can present with a variety of life-threatening complications.

Patient concerns:
The patients visited our hospital due to abdominal pain and anemia, and had chronic pancreatitis as an underlying disease.

Diagnoses:
Computed tomography showed a large gastroduodenal artery pseudoaneurysm, arterioportal vein fistula, and portal vein stenosis.

Interventions:
We would like to report the successful use of the coils, and N-butyl cyanoacrylate glue for the therapeutic embolization of the pseudoaneurysm and fistula between the gastroduodenal artery and the portal vein, and stenting for portal vein stenosis.

Outcomes:
On the day following the endovascular management, the patient reported remission of abdominal pain, and hemoglobin level returned to normal after transfusion. It was confirmed that it was still well maintained in the follow-up examination after 1 month.

Lessons:
Although chronic pancreatitis causes many vascular complications, simultaneous occurrence of these lesions is extremely rare. Herein, we share our experience with a unique case of an extrahepatic arterioportal fistula induced by the rupture of gastroduodenal artery pseudoaneurysm with concomitant portal vein stenosis. In these complex cases, combined transarterial embolization and transportal stenting can be helpful.
Behavioral Management of Children With Autism in the Emergency Department
Authors: Wolpert Katherine H. , Kodish Ian , Kim Soo-Jeong , Uspal Neil G. 
Published: December 2022

Abstract
Autism spectrum disorder (ASD) is characterized by impaired social communication in conjunction with patterned behaviors. Often associated with emotional dysregulation, irritability, aggression, depression, and suicidality, ASD youth frequently present to the emergency department for behavioral and mental health evaluation. Psychiatric comorbidities, agitation, and depression are commonly encountered. During these visits, practitioners must thoughtfully consider organic etiologies for presenting symptoms, formulate plans to address risk of agitation, and understand how to effectively formulate disposition options in this patient population.
Multi-scale tissue fluorescence mapping with fibre optic ultraviolet excitation and generative modelling
Authors: Ang Joel Lang Yi , Tan Ko Hui , Yong Alexander Si Kai , Tan Chiyo Wan Xuan , Kng Jessica Sze Jia , Tan Cyrus Jia Jun , Soh Rachael Hui Kie , Tan Julian Yi Hong , Liang Kaicheng 
Published: December 2022

AbstractCellular imaging of thick samples requires physical sectioning or laser scanning microscopy, generally incompatible with high-throughput requirements. We developed fibre optic microscopy with ultraviolet (UV) surface excitation (FUSE), a portable, quantitative fluorescence imaging platform for thick tissue that substantially advances prior UV excitation approaches with illumination engineering and computational methods. Optic fibres delivered <300nm light with directional control, enabling unprecedented 50X widefield imaging on thick tissue with sub-nuclear clarity, and 3D topography of surface microstructure. Generative modelling of high-magnification images using our normalising flow architecture FUSE-Flow (open-source) enhanced low-magnification imaging by variational inference. Comprehensive validation comprised multi-scale fluorescence histology compared with standard H&E, and quantitative analyses of senescence, antibiotic toxicity, and nuclear DNA content in tissue models via efficient sampling of entire murine organs by thick slices up to 0.4×8×12mm and 1.3 million cells per surface. This technology addresses long-standing laboratory gaps for high-throughput studies for rapid cellular insights.TeaserLarge-field functional cellular insights into thick tissue with generative AI enables accelerated decision-making
Redesigning methionyl-tRNA synthetase forβ-methionine activity with adaptive landscape flattening and experiments
Authors: Opuu Vaitea , Nigro Giuliano , Lazennec-Schurdevin Christine , Mechulam Yves , Schmitt Emmanuelle , Simonson Thomas 
Published: December 2022

AbstractAmino acids (AAs) with a noncanonical backbone would be a valuable tool for protein engineering, enabling new structural motifs and building blocks. To incorporate them into an expanded genetic code, the first, key step is to obtain an appropriate aminoacyl-tRNA synthetase (aaRS). Currently, directed evolution is not available to optimize such AAs, since an appropriate selective pressure is not available. Computational protein design (CPD) is an alternative. We used a new CPD method to redesign MetRS and increase its activity towardsβ-Met, which has an extra backbone methylene. The new method considered a few active site positions for design and used a Monte Carlo exploration of the corresponding sequence space. During the exploration, a bias energy was adaptively learned, such that the free energy landscape of the apo enzyme was flattened. Enzyme variants could then be sampled, in the presence of the ligand and the bias energy, according to theirβ-Met binding affinities. Eleven predicted variants were chosen for experimental testing; all exhibited detectable activity forβ-Met adenylation. Top predicted hits were characterized experimentally in detail. Dissociation constants, catalytic rates, and Michaelis constants for bothα-Met andβ-Met were measured. The best mutant retained a preference forα-Met overβ-Met; however, the preference was reduced, compared to the wildtype, by a factor of 29. For this mutant, high resolution crystal structures were obtained in complex with bothα-Met andβ-Met, indicating that the predicted, active conformation ofβ-Met in the active site was retained.Author summaryAmino acids (AAs) with a noncanonical backbone would be valuable for protein engineering, enabling new structural motifs. To incorporate them into an expanded genetic code, the key step is to obtain an appropriate aminoacyl-tRNA synthetase (aaRS). Currently, directed evolution is not available to optimize such AAs. Computational protein design is an alternative. We used a new method to redesign MetRS and increase its activity towardsβ-Met, which has an extra backbone methylene. The method considered a few active site positions for design and used a Monte Carlo exploration of sequence space, during which a bias energy was adaptively learned, such that the free energy landscape of the apo enzyme was flattened. Enzyme variants could then be sampled, in the presence of the ligand and the bias energy, according to theirβ-Met binding affinities. Eleven predicted variants were chosen for experimental testing; all exhibited detectableβ-Met adenylation activity. Top hits were characterized experimentally in detail. The best mutant had its preference forα-Met overβ-Met reduced by a factor of 29. Crystal structures indicated that the predicted, active conformation ofβ-Met in the active site was retained.
PKA regulatory subunit Bcy1 couples growth, lipid metabolism, and fermentation during anaerobic xylose growth inSaccharomyces cerevisiae
Authors: Wagner Ellen R. , Nightingale Nicole M. , Jen Annie , Overmyer Katherine A. , McGee Mick , Coon Joshua J. , Gasch Audrey P. 
Published: December 2022

AbstractOrganisms have evolved elaborate physiological pathways that regulate growth, proliferation, metabolism, and stress response. These pathways must be properly coordinated to elicit the appropriate response to an ever-changing environment. While individual pathways have been well studied in a variety of model systems, there remains much to uncover about how pathways are integrated to produce systemic changes in a cell, especially in dynamic conditions. We previously showed that deletion of Protein Kinase A (PKA) regulatory subunitBCY1can decouple growth and metabolism inSaccharomyces cerevisiaeengineered for anaerobic xylose fermentation, allowing for robust fermentation in the absence of division. This provides an opportunity to understand how PKA signaling normally coordinates these processes. Here, we integrated transcriptomic, lipidomic, and phosphor-proteomic responses upon a glucose to xylose shift across a series of strains with different genetic mutations promoting either coupled or decoupled xylose-dependent growth and metabolism. Together, results suggested that defects in lipid homeostasis limit growth in thebcy1Δstrain despite robust metabolism. To further understand this mechanism, we performed adaptive laboratory evolutions to re-evolve coupled growth and metabolism in thebcy1Δparental strain. Genetic mutations in PKA subunitTPK1and lipid regulatorOPI1, among other genes underscored a role for lipid homeostasis, which was further supported by evolved changes in lipid profiles and gene expression. We suggest several models for how cells coordinate growth, metabolism, and other responses in budding yeast and how restructuring these processes enables anaerobic xylose utilization.Author SummaryAll organisms utilize an energy source to generate the cellular resources needed to grow and divide. These individual processes have been well study, but the coordination and crosstalk between the process is not well understood. To study growth and metabolism coupling, we used a yeast strain that was genetically engineered to ferment the sugar xylose but lacked growth on the sugar. The decoupled growth and metabolism was caused by a single gene deletion in a highly conserved signaling pathway found in all eukaryotes. While our work is focused on xylose metabolism, we address the fundamental question of how cells coordinate growth with metabolism under non-ideal conditions. We identified vast changes in gene expression that implicated altered regulatory mechanisms involved in lipid metabolism correlating with decouple growth and metabolism. Our work highlights the complexity of engineering new cellular functions and that global regulatory modifications, rather than altering individual pathways, may be required for broad cellular changes.
Forces driving transposable element load variation during Arabidopsis range expansion
Authors: Jiang Juan , Xu Yong-Chao , Zhang Zhi-Qin , Chen Jia-Fu , Niu Xiao-Min , Hou Xing-Hui , Li Xin-Tong , Wang Li , Zhang Yong , Ge Song , Guo Ya-Long 
Published: December 2022

ABSTRACTGenetic load refers to the accumulated and potentially life-threatening deleterious mutations in populations. Understanding the mechanisms underlying genetic load variation of transposable elements (TEs), one major large-effect mutations, during range expansion is an intriguing question in biology. Here, we used 1,115 globally natural accessions ofArabidopsis thaliana, to study the driving forces of TE load variation during its range expansion. The TE load increased with range expansion, especially in the recently established Yangtze River basin population. The effective population size explained 62.0% of the variance in TE load, and high transposition rate and positive selection or hitch-hiking effect contributed to the accumulation of TEs in the expanded populations. We genetically mapped the candidate causal genes or TEs and revealed the genetic architecture of TE load. Overall, this study reveals the variation in the genetic load of TEs during Arabidopsis expansion and highlights the causes of TE load variation.
Human TRMT2A methylates tRNA and contributes to translation fidelity
Authors: Witzenberger Monika , Burczyk Sandra , Settele David , Mayer Wieland , Welp Luisa M. , Heiss Matthias , Wagner Mirko , Monecke Thomas , Janowski Robert , Carell Thomas , Urlaub Henning , Hauck Stefanie M. , Voigt Aaron , Niessing Dierk 
Published: December 2022

AbstractMethyl-5-uridine (m5U) is one of the most abundant RNA modifications found in cytosolic tRNA. tRNA methyltransferase 2 homolog A (hTRMT2A) is the dedicated mammalian enzyme of m5U formation at tRNA position 54. However, its RNA binding specificity and functional role in the cell are not well understood. Here we dissected structural and sequence requirements for binding and methylation of its RNA targets. Specificity of tRNA modification by TRMT2A is achieved by a combination of modest binding preference and presence of a uridine in position 54 of tRNAs. Mutational analysis together with crosslinking experiments identified a large hTRMT2A-tRNA binding surface. Furthermore, complementing hTRMT2A interactome studies revealed that TRMT2A interacts with proteins involved in RNA biogenesis. Finally, we addressed the question of the importance of TRMT2A function by showing that its knockdown reduces translation fidelity. These findings extend the role of hTRMT2A beyond tRNA modification towards a role in translation.
High Field Asymmetric Waveform Ion Mobility Spectrometry-Mass Spectrometry to Enhance Cardiac Muscle Proteome Coverage
Authors: Ai Lizhuo , Binek Aleksandra , Kreimer Simion , Ayres Matthew , Stotland Aleksandr , Van Eyk Jennifer E. 
Published: December 2022

AbstractHeart tissue sample preparation for mass spectrometry (MS) analysis that includes pre-fractionation reduces the cellular protein dynamic range and increases the relative abundance of non-sarcomeric proteins. We previously described “IN-Sequence” (IN-Seq) where heart tissue lysate is sequentially partitioned into three subcellular fractions to increase the proteome coverage than a single direct tissue analysis by mass spectrometry. Here, we report an adaptation of the high-field asymmetric ion mobility spectrometry (FAIMS) coupled to mass spectrometry, and the establishment of a simple one step sample preparation coupled with gas-phase fractionation. FAIMS approach substantially reduces manual sample handling, significantly shortens MS instrument processing time, and produces unique protein identification and quantification approximating the commonly used IN-Seq method in for less time requirement.
Annexin A6 mediates calcium-dependent secretion of exosomes
Authors: Williams Justin Krish , Ngo Jordan Matthew , Schekman Randy 
Published: December 2022

AbstractExosomes are a subtype of extracellular vesicles (EV) that are secreted upon fusion of multivesicular bodies (MVBs) with the plasma membrane. In order to identify proteins responsible for this fusion event, we developed sensitive cellular and cell-free assays to measure exosome secretion. Our results suggest that exosome secretion is coupled to Ca2+-dependent plasma membrane repair. Using a targeted proteomics approach, we identify annexin A6 (ANXA6), a well-known plasma membrane repair protein, as a MVB binding protein and demonstrate that it is required for Ca2+-dependent exosome secretion both in intact and in permeabilized cells. Our results suggest that cells employ MVBs as a membrane source for plasma membrane lesion repair during physiological mechanical stress or treatment with a bacterial pore-forming toxin, and that this repair process results in the coincident secretion of exosomes. We propose that this unconventional secretion process may contribute significantly to the heterogeneity of EVs in biological fluids.
Sex and age specific effects on the development of addiction and compulsive-like drinking in rats
Authors: Foo Jerome C. , Skorodumov Ivan , Spanagel Rainer , Meinhardt Marcus W. 
Published: December 2022

AbstractBiological factors are known to influence disease trajectories and treatment effectiveness in alcohol addiction. One important biological factor influencing disease dynamics in alcohol use disorder is sex, as preclinical and clinical evidence suggests that gender influences disease trajectories in subjects with alcohol use disorder. Another critical factor is age at first intoxicating drink, which has been identified as a risk factor for later alcohol binging. Preclinical research allows prospective monitoring of rodents throughout the lifespan, providing very detailed information that cannot be acquired in humans. Lifetime monitoring in rodents can be conducted under highly controlled conditions, during which one can systematically introduce multiple biological and environmental factors that impact behaviors of interest.Here, we used the alcohol deprivation effect (ADE) rat model of alcohol addiction in a computerized drinkometer system to study disease dynamics and transition points of addictive behavior in cohorts of adolescent vs. adult as well as male vs. female rats in direct comparison. Early age of onset of drinking (postnatal day 40) in male rats had surprisingly little impact on the development of drinking behavior and compulsivity (quinine taste adulteration) when compared to rats that started drinking during early adulthood (postnatal day 72). However, female rats showed a stronger resistance to quinine taste adulteration after a history of ADEs, indicative of more pronounced compulsive behavior in females as compared to male rats. Specifically, female rats exhibit during quinine taste adulteration a higher frequency of drinking events and a larger access size, especially from the 20% alcohol solution, resulting in fast intoxication despite taste aversion in a relapse-like situation. Our results suggest that drinking patterns, and specifically solution preference as well as access size, provide a valuable indication into determining compulsive drinking. These findings provide a better understanding of sex and age factors involved in the development of drinking behavior that are important in preclinical development of models of addiction, drug development and exploration options for new treatments.