bluuebunny/crossref_metadata_2025_split huggingface dataset:
Knowledge inheritance in disciplines: Quantifying the successive and distant reuse of references
Authors: Zhou Hongyu , Dong Ke , Xia Yikun 
Published: September 2023

AbstractHow the knowledge base of disciplines grows, renews, and decays informs their distinct characteristics and epistemology. Here we track the evolution of knowledge bases of 19 disciplines for over 45 years. We introduce the notation of knowledge inheritance as the overlap in the set of references between years. We discuss two modes of knowledge inheritance of disciplines—successive and distant. To quantify the status and propensity of knowledge inheritance for disciplines, we propose two indicators: one descriptively describes knowledge base evolution, and one estimates the propensity of knowledge inheritance. When observing the continuity in knowledge bases for disciplines, we show distinct patterns for STEM and SS&H disciplines: the former inherits knowledge bases more successively, yet the latter inherits significantly from distant knowledge bases. We further discover stagnation or revival in knowledge base evolution where older knowledge base ceases to decay after 10 years (e.g., Physics and Mathematics) and are increasingly reused (e.g., Philosophy). Regarding the propensity of inheriting prior knowledge bases, we observe unanimous rises in both successive and distant knowledge inheritance. We show that knowledge inheritance could reveal disciplinary characteristics regarding the trajectory of knowledge base evolution and interesting insights into the metabolism and maturity of scholarly communication.
Ag6Cu8(C=CAr)14(DPPB)2: A Rigid Ligand Co‐Protected Bimetallic Silver(I)‐Copper(I) Cluster with Room‐Temperature Luminescence
Authors: Jin Jun‐Ling , Zhang Sheng‐Fa , Zhao Pei , Shen Yang‐Lin , Fang Jun‐Jie , Liu Zheng , Ehara Masahiro , Mi Li‐Wei , Xie Yun‐Peng , Lu Xing 
Published: September 2023

AbstractMetal clusters have become increasingly important in various applications, with ligands playing a crucial role in their construction. In this study, we synthesized a bimetallic cluster, Ag6Cu8(C=CAr)14(DPPB)2 (Ag6Cu8), using a rigid acetylene ligand, 3,5‐bis(trifluoromethyl)phenylacetylide. Through single‐crystal structure characterization, we discovered that the butterfly‐shaped Ag2Cu2 motifs were subject to distortion due to steric hindrance imposed by the rigid ligand. These motifs assembled together through shared vertices and edges. Mass spectrometry analysis revealed that the primary fragments detected during electrospray ionization (ESI) testing corresponded to the Ag2Cu2 motifs. Furthermore, we conducted a comprehensive investigation of the cluster's solution properties employing 31P NMR, UV‐vis absorption, and photoluminescent measurements. In contrast to previously reported Ag/Cu bimetallic clusters protected by flexible ligands, Ag6Cu8 protected by rigid ligands exhibited intriguing room temperature fluorescence properties alongside excellent thermal stability. DFT calculations on Ag6Cu8 and Ag6Cu8 with the rigid aromatic ring removed revealed that the presence of the rigid aromatic ring can lower the electronic energy levels of the cluster, and reduce the energy gap from 4.05 eV to 3.45 eV. Moreover, the rigid ligand further suppressed the non‐radiative transition process, leading to room temperature fluorescence emission.
CEO tenure and environmental fraud for listed family firms
Authors: Wang Yang , Zhang Yifei , Chang Xiao , Kang Wei 
Published: September 2023

AbstractThis paper examines CEO tenure's impact on the environmental fraud committed by listed Chinese family firms from the year 2012 to 2019. Using a bivariate probit model, we provide robust evidence that CEO tenure is positively related to the environmental fraud commission, indicating that longer‐serving CEOs are more likely to violate environmental regulations and to commit fraud in the family firms. Besides, when there is a higher proportion of independent directors, CEO tenure is negatively related to the environmental fraud commission but positively related to fraud detection. Moreover, when there is a greater proportion of family members in senior positions, CEOs with longer tenure are more likely to commit environmental fraud. Our additional analysis finds that compared to non‐family professional CEOs, family CEOs are more likely to commit environmental fraud. Overall, our results call for the introduction of CEO tenure limits and the increased recruitment of independent directors in family firms.
Personalized additive manufacturing of devices for the management of enteroatmospheric fistulas
Authors: Calero Castro Francisco José , Padillo Eguía Andrés , Durán Muñoz‐Cruzado Virginia , Tallón Aguilar Luis , Tinoco González José , Laga Imán , de la Portilla de Juan Fernando , Pareja Ciuró Felipe , Padillo Ruiz Javier 
Published: September 2023

AbstractAdditive manufacturing techniques allow the customized design of medical devices according to the patient's requirements. Enteroatmospheric fistula is a pathology that benefits from this personalization due to its extensive clinical variability since the size and morphology of the wound differ extensively among patients. Standard prosthetics do not achieve proper isolation of the wound, leading to a higher risk of infections. Currently, no effective personalized technique to isolate it has been described. In this work, we present the workflow for the design and manufacture of customized devices adapted to the fistula characteristics as it evolves and changes during the treatment with Negative Pressure Wound Therapy (NPWT). For each case, a device was designed with dimensions and morphology depending on each patient's requirements using white light scanning, CAD design, and additive manufacturing. The design and manufacture of the devices were performed in 230.50 min (184.00–304.75). After the placement of the device, the wound was successfully isolated from the intestinal content for 48–72 h. The therapy was applied for 27.71 ± 13.74 days, and the device was redesigned to adapt to the wound when geometrical evolutionary changes occur during the therapy. It was observed a decrease in weekly cures from 23.63 ± 10.54 to 2.69 ± 0.65 (p = 0.001). The fistulose size was reduced longitudinal and transversally by 3.25 ± 2.56 cm and 6.06 ± 3.14 cm, respectively. The wound depth also decreased by 1.94 ± 1.08 cm. In conclusion, customization through additive manufacturing is feasible and offers promising results in the generation of personalized devices for the treatment of enteroatmospheric fistula.
Exploring the application value of pro‐gastrin‐releasing peptide in the clinical diagnosis and surgical treatment of medullary thyroid carcinoma
Authors: Miao Qiang , Lv Xiaohui , Luo Li , Zhang Junlong , Cai Bei 
Published: September 2023

AbstractObjectiveTo investigate the relationship between pro‐gastrin‐releasing peptide (ProGRP) and the clinical characteristics of patients with medullary thyroid carcinoma (MTC) and the value of ProGRP in surgical treatment monitoring.Patients and MethodsA total of 347 patients with MTC and non‐MTC malignant and benign thyroid diseases were enrolled. The concentrations of neuron‐specific enolase (NSE), carcinoembryonic antigen (CEA), calcitonin (CT), and ProGRP were determined by Elecsys® assays. The NSE, CEA, CT, and ProGRP levels in different thyroid disease groups were compared, and ProGRP levels in different clinicopathological feature groups pre and postoperatively were further compared.ResultsThe CT, CEA, NSE, and ProGRP levels were upregulated in the MTC group compared to those in the non‐MTC malignant and benign thyroid disease groups. The area under the receiver operating characteristic curve (AUC) of ProGRP for the diagnosis of MTC was 0.832(0.787–0.871), similar to that of CT and CEA. The sensitivity and specificity were 71.4% and 92.7%, respectively, and the optimal cut‐off value was 61.8 pg/mL. The AUC of ProGRP combined with CT or CEA for the diagnosis of MTC was 0.933 (0.900–0.958) and 0.922 (0.886–0.949), respectively, which were higher than those of a single index. ProGRP levels were higher in patients with lymph nodes and distant metastases than in patients without metastases. The postoperative level of ProGRP was lower than that before treatment.ConclusionProGRP is comparable to CEA and CT as an MTC biomarker with broad prospects. It has potential application value in the progression of MTC assessment and the evaluation of surgical intervention effects.
A qualitative content analysis of ChatGPT's client simulation role‐play for practising counselling skills
Authors: Maurya Rakesh K. 
Published: September 2023

AbstractRole‐play is often used as a pedagogical technique by counsellor educators to help counsellors‐in‐training (CITs) learn and practise counselling skills. This study used qualitative content analysis to assess the effectiveness of Chat Generative Pre‐Trained Transformer (ChatGPT), an artificial intelligence (AI) chatbot, in simulating client role‐play to facilitate practising counselling skills. The author used 10 case vignettes to conduct a simulated role‐play where ChatGPT was asked to simulate the client and author as counsellor. The results indicated that ChatGPT demonstrated authenticity, consistency, appropriate emotional expression, cultural sensitivity, empathy and self‐awareness while portraying the client. However, at times, ChatGPT's responses to the counsellor's questions appeared overly idealised, lacking the authenticity typically observed in real clients. Furthermore, ChatGPT, as a simulated client, lacked non‐verbal cues, limiting the overall experience of counselling skill practice. Recommendations for CITs and future research are discussed.
Phytochemical and Pharmacological Aspects of Psoralen – A Bioactive Furanocoumarin fromPsoralea corylifoliaLinn.
Authors: Tripathi Nancy , Bhardwaj Nivedita , Kumar Sanjay , Jain Shreyans K. 
Published: September 2023

AbstractSince long ago, medicinal plants have played a vital role in drug discovery. Being blessed and rich in chemovars with diverse scaffolds, they have unique characteristics of evolving based on the need. The World Health Organization also mentions that medicinal plants remain at the center for meeting primary healthcare needs as the population relies on them. The plant‐derived natural products have remained an attractive choice for drug development owing to their specific biological functions relevant to human health and also the high degree of potency and specificity they offer. In this context, one such esteemed phytoconstituent with inexplicable biological potential is psoralen, a furanocoumarin. Psoralen was the first constituent isolated from the plantPsoralea corylifolia, commonly known as Bauchi. Despite being a life‐saver for psoriasis, vitiligo, and leukoderma, it also showed immense anticancer, anti‐inflammatory, and anti‐osteoporotic potential. This review brings attention to the possible application of psoralen as an attractive target for rational drug design and medicinal chemistry. It discusses the various methods for the total synthesis of psoralen, its extraction, the pharmacological spectrum of psoralen, and the derivatization done on psoralen.
A Pair of Undescribed Alkaloid Enantiomers from Marine Sponge‐Derived Fungus Hamigera avellanea and Their Antimicrobial and Cytotoxic Activities
Authors: Minh Le Thi Hong , Anh Nguyen Mai , Huyen Vu Thi Thu , Quyen Vu Thi , Dao Phi Thi , Huong Doan Thi Mai , Cuong Pham Van , Tuan Cao Duc , Kiem Phan Van , Tai Bui Huu 
Published: September 2023

AbstractFrom marine sponge‐associated fungus Hamigera avellanea, thirteen secondary metabolites including a pair of undescribed alkaloid enantiomers (+)‐hamiavemin A (4S) (+)‐1 and (−)‐hamiavemin A (4R) (−)‐1. Compound 1 was enantiomers resolved by the Chiralpak AS‐3 column, using a hexane/isopropanol mobile phase. Their structures were determined based on extensive analyses of HR‐ESI‐MS, 1D and 2D NMR spectra. The absolute configuration of (+)‐1 and (−)‐1 were assigned tentatively by ECD calculations. Among the isolates, compound 6 showed strongest antibacterial activity against Enterococcus faecalis, Staphylococcus aureus, Bacillus cereus, Escherichia coli, Salmonella enterica, and Candida albicans with the MIC values of 2, 2, 16, 32, 64, and 16 μg/mL, respectively, which were stronger than that of the positive control compound, kanamycin (MIC values ranging from 4 to 128 μg/mL). In addition, compounds 1, 2, and 9 showed moderate cytotoxic activity against three cancer cell lines, HepG2, A549, and MCF‐7 with the IC50 values ranging from 55.35±1.70 to 83.02±2.85 μg/mL.
Reaction Pathways for Synthesis of Four Carbon Chemicals from Sugars and Sugar Derived Platform Chemicals
Authors: Palai Yayati Naresh , Fukuoka Atsushi , Shrotri Abhijit 
Published: September 2023

AbstractChemicals with a four‐carbon chain (C4 chemicals), like succinic acid, maleic acid, 1,4‐butanediol, and tetrahydrofuran are extensively used as commodity chemicals and polymer precursors. Currently, these chemicals are commercially synthesized from petrochemical based feedstocks. There is a growing interest in catalytic pathways for producing these and other C4 chemicals from biomass. However, biomass is mainly composed of hexose and pentose sugars and the natural abundance of a C4 feedstock is low. This review summarizes the current development in catalytic pathways for C4 chemical synthesis from biomass derived sugars and its derivatives and suggests future direction of research.
The Ring‐Opening Reaction of Benzofuran Involving the Cleavage of C−O Bond
Authors: Yu Jiahui , Huang Liangbin 
Published: September 2023

AbstractRing‐opening of (di)benzofurans is a significant area of research in organic chemistry, offering versatile and direct synthetic strategies to access valuable functional phenol derivatives. Transition metal catalysis, particularly nickel‐catalyzed reactions, has been extensively explored for the selective cleavage of the C−O bond in (di)benzofuran. Metal‐free methods, such as acid catalysis and strong base‐mediated process, have also emerged as important alternatives. Organometallic reagents, including Grignard and organolithium reagents, play a pivotal role in promoting efficient C−O bond activation. The field of (di)benzofuran ring opening holds great promise for the synthesis of complex molecules with diverse applications in pharmaceuticals, materials science, and fine chemical synthesis. Continued research efforts will pave the way for innovative strategies and broaden the utility of (di)benzofuran derivatives in various fields.
Synthesis and Enzymatic Conversion of Amino Acids Equipped with the Silanetriol Functionality: A Prelude to Silicon Biodiversification
Authors: Dussart‐Gautheret Jade , Rivollier Julie , Simon Cédric , De Simone Alessandro , Berthet Jérôme , Delbaere Stéphanie , Maruchenko Régina , Troufflard Claire , Lesage Denis , Gimbert Yves , Lemière Gilles , Marlière Philippe , Fensterbank Louis 
Published: September 2023

AbstractSynthetic routes are reported for the three analogues of the simplest L‐2‐amino‐dicarboxylic acids, aspartate, glutamate, and aminoadipate, in which the silanetriol group (Si(OH)3) replaces the distal carboxyl group (CO2H). Direct access to the silanetriol amino acids relied either on catalytic hydrosilylation of a terminal alkene using triethoxysilane, or on alkylation of a glycine equivalent anion by triallyl(iodomethyl)silane. In both cases, acid hydrolysis afforded the silanetriol amino acids. These were shown to self‐assemble into siloxane Si‐O clusters as their concentration in water increased in the pH range of 1–12. Such reversible cross‐linking did not prevent silanetriol amino acids from serving as substrates of an aminotransferase enzyme, boding well for their utilization as microbial nutrients to encompass silicon in future stages of metabolism and polypeptide edifices.
Collective Synthesis of Sarpagine and Macroline Alkaloid‐Inspired Compounds
Authors: Aoyama Hikaru , Davies Caitlin , Liu Jie , Pahl Axel , Kirchhoff Jan‐Lukas , Scheel Rebecca , Sievers Sonja , Strohmann Carsten , Grigalunas Michael , Waldmann Herbert 
Published: September 2023

AbstractDesign strategies that can access natural‐product‐like chemical space in an efficient manner may facilitate the discovery of biologically relevant compounds. We have employed a divergent intermediate strategy to construct an indole alkaloid‐inspired compound collection derived from two different molecular design principles, i.e. biology‐oriented synthesis and pseudo‐natural products. The divergent intermediate was subjected to acid‐catalyzed or newly discovered Sn‐mediated conditions to selectively promote intramolecular C‐ or N‐acylation, respectively. After further derivatization, a collection totalling 84 compounds representing four classes was obtained. Morphological profiling via the cell painting assay coupled with a subprofile analysis showed that compounds derived from different design principles have different bioactivity profiles. The subprofile analysis suggested that a pseudo‐natural product class is enriched in modulators of tubulin, and subsequent assays led to the identification of compounds that suppress in vitro tubulin polymerization and mitotic progression.
Inhibitory Effect of Salicin on Staphylococcus aureus Coagulase
Authors: Jiang Yijing , Hou Juan , Liu Chang , Zhao Chunhui , Xu Yangming , Song Wu , Shu Zunhua , Wang Bingmei 
Published: September 2023

AbstractThe massive use of antibiotics has resulted in an alarming increase in antibiotic resistance in Staphylococcus aureus (S. aureus). This study aimed to identify the inhibitory effect of salicin on S. aureus. Coagulase (Coa) activity was assessed using in vitro Coa assays and Western blot, thermal shift assay (TSA), fluorescence quenching and molecular docking experiments were conducted to verify the interaction between salicin and Coa. An in vivo mouse pneumonia model demonstrated that salicin can reduce the virulence of S. aureus. In vitro Coa assays elucidated that salicin directly inhibited Coa activity. The Western blot and TSA results suggested that salicin did not block the expression of Coa but affected the thermal stability of the protein by binding to Coa. The fluorescence quenching, molecular docking and molecular dynamics assays have found that the most promising binding site between salicin and Coa was GLN‐97. The pneumonia model of mice infected with S. aureus revealed that salicin could not only reduce the content of lung bacteria in mice but also prolong their survival. Salicin was identified as a novel anti‐infective candidate compound with the potential to target Coa and inhibit its activity by binding to it, which would facilitate the development of roadmaps for future research.
Pincer Ligands as Multifunctional Agents for Alzheimer's Copper Dysregulation and Oxidative Stress: A Computational Evaluation
Authors: Puentes‐Díaz Nicolás , Chaparro Diego , Morales‐Morales David , Alí‐Torres Jorge , Flores‐Gaspar Areli 
Published: September 2023

AbstractAlzheimer's disease (AD) is the most common form of dementia worldwide, affecting millions of people around the globe. AD is characterized by different pathologies being beta‐amyloid (Aβ) plaque formation, metal ion dysregulation, and oxidative stress (OS) central topics under investigation. Copper‐Aβ complexes have been shown to induce catalytic hydrogen peroxide formation and increase OS in the brain leading to neuronal death. Pincer‐type compounds are tridentate ligands that coordinate metals in a planar fashion whose properties can be tuned via group substitutions, giving rise to many possibilities in catalysis and drug discovery. In this work we evaluated the potential pharmaceutical activity of 26 pincer compounds in AD's copper ion‐related oxidative stress framework. In this sense, four key aspects were considered: 1) Lipinski's rule of five, 2) blood‐brain barrier permeation, 3) standard reduction potential (SRP) of the formed copper complexes, and 4) the ligand's affinity towards copper cations. The evaluation of these criteria was performed by means of bioinformatic tools and electronic structure calculations at the DFT level of theory. Our results suggest that two compounds from this set are potential antioxidant agents, whereas five of them are promissory distributor‐like compounds in the context of AD.
FLASH Genome Editing Pipeline: An Efficient and High‐Throughput Method to Construct Arrayed CRISPR Library for Plant Functional Genomics
Authors: Yao Lu , Wang Xiaochun , Ke Runnan , Chen Kaiyuan , Xie Kabin 
Published: September 2023

AbstractCRISPR/Cas9 genome editing is a revolutionary technology for plant functional genomics and crop breeding. In this system, the Cas9 nuclease is directed by a guide RNA (gRNA) to cut the DNA target and introduce mutation through error‐prone DNA break repair. Owing to its simplicity, CRISPR/Cas9‐mediated targeted gene knockout is widely used for high‐throughput genetic screening in animal cell cultures and bacteria. However, high‐throughput genetic screening using CRISPR/Cas9 is still challenging in plants. We recently established a new approach, named the FLASH genome editing pipeline, to construct an arrayed CRISPR library in plants. In this pipeline, a set of 12 PCR fragments with different lengths (referred to as FLASH tags) are used to index the Cas9/gRNA vectors. Subsequently, a mixture of 12 Agrobacterium strains, in which each strain contained a FLASH‐tag indexed vector, was transformed into rice plants. As a result, a unique link between the target gene/gRNA and FLASH tag is generated, which allows reading gRNA information in bacterial strains and gene‐edited plants using regular PCR and gel electrophoresis. This protocol includes step‐by‐step instructions for gRNA design, high throughput assembly of FLASH‐tag indexed Cas9/gRNA plasmids, Agrobacterium‐mediated transformation of 12 indexed plasmids, and fast assignment of target gene information in primary transformants. The arrayed CRISPR library described here is suitable for small‐ to large‐scale genetic screening and allows fast and comprehensive gene function discovery in plants. © 2023 Wiley Periodicals LLC.Basic Protocol 1: Assembly of FLASH‐tag‐indexed Cas9/gRNA plasmidsBasic Protocol 2: Preparation of the Cas9/gRNA plasmid libraryBasic Protocol 3: Library preparation of Agrobacterium strains and mixing FLASH‐tag indexed strainsBasic Protocol 4: Grouped transformation and assignments of gRNA information of gene‐edited plants
Facile Synthesis of Large Bulk Crystal Based on Perovskite‐Quantum Dots Hybrid Structure
Authors: Han Jianhua , Zhao Shuang , Wang Zengyi , Yan Huiyu , Lin Hong , Su Yuyu 
Published: September 2023

AbstractIn this study, the fabrication of a large bulk crystal based on the hybrid structure of CH3NH3PbBr3 (MAPBr) and PbS quantum dots (QDs) with excellent crystal lattice match using an in situ epitaxial crystallization technique is presented. The hybrid bulk crystal is successfully constructed by uniformly dispersing PbS QDs within the MAPBr matrix. The crystalline growth method is also changed to achieve the desired hybrid structure. The light emission range is modulated and the light emission lifetime is prolonged by constructing a MAPBr‐PbS hybrid structure. Additionally, the hybrid bulk crystal exhibited an up‐conversion effect, which does not appear in MAPBr single crystal. These findings highlight the promising potential of the MAPBr‐PbS QDs hybrid bulk crystal in the fields of optics and photoelectronic, such as all solid‐state lasers, bio‐imaging, optical data storage, and photovoltaic applications. This in situ hybrid crystallization technique also provides a universal pathway for the preparation of other bulk crystals based on perovskite‐QDs hybrid structures.