bluuebunny/crossref_metadata_2025_split huggingface dataset:
scRNA-seq reveals the diversity of the developing cardiac cell lineage and molecular building blocks of the primary pacemaker
Authors: Nahia Karim Abu , Sulej Agata , Migdał Maciej , Ochocka Natalia , Ho Richard , Kamińska Bożena , Zagorski Marcin , Winata Cecilia L. 
Published: June 2023

AbstractThe heart is comprised of a variety of specialized cell types that work in unison to maintain blood flow. Here we utilized scRNA-seq analysis to delineate the diversity of cardiac cell types in the zebrafish. With the growing use of the zebrafish to model human heart biology, a deeper insight into its complex cellular composition is critical for a better understanding of heart function, development, and associated malformations. We present a high resolution atlas of zebrafish heart single cells transcriptomics, consisting of over 50 000 cells representing the building blocks of the zebrafish heart at 48 and 72 hpf. We defined 18 discrete cell populations comprising major cell lineages and sublineages of the developing heart. We pinpointed a population of cells likely to be the primary pacemaker and identified the transcriptome profile defining this critical cell type. Our analyses identified two genes,atp1b3bandcolec10, which were enriched in the sinoatrial pacemaker cells. CRISPR/Cas9-mediated knockout of these two genes significantly reduced heart rate which is accompanied by arrhythmia or morphological defects, suggesting their novel function in cardiac development and conduction. Additionally, we describe other subpopulations of cardiac cell lineages, including the endothelial and neural cells, whose expression profiles we provide as a resource for further investigations into the cellular and molecular mechanisms of this organ.
Leveraging human resource management practices during mergers and acquisitions: a bibliometric analysis and future research agenda
Authors: Budhiraja Sunil , Thakur Mahima , Yadav Mohini 
Published: June 2023

PurposeDespite enormous literature on Human Resource Management (HRM) practices in the context of Mergers and Acquisitions (M&As), researchers have not come up with a synthesis that integrates the role and importance of HRM practices during M&As. This study aims to identify, analyse and synthesise existing literature to discover associated HRM practices that facilitate organisational change during M&As.Design/methodology/approachBibliometric analysis is performed using 473 research articles entailing 871 authors from 62 countries (published in Scopus and WoS listed journals), followed by a thematic cluster analysis using bibliographic coupling. The analysis is performed using different means as citation analysis, cluster analysis and keyword analysis to reveal the most significant publications, authors, keywords, trends and future research questions.FindingsThe results are primarily descriptive and aim at capturing a panoramic view of what was already written on the topic so far. The bibliometric analysis is conducted using different means like citation analysis, cluster analysis and keyword analysis to reveal the most significant publications, notable authors, keywords, current research trends and future research questions. Further, the bibliographic coupling analysis led to the identification of the following six clusters: (1) coping strategies during and post-M&As; (2) changes in individual and organisational identification during and post-M&As; (3) role of cultural and transformational leadership in M&As success; (4) HRM practices to develop employee capabilities post-M&As; (5) case studies and success stories of M&As; and (6) organisational readiness for M&As.Practical implicationsThis study has theoretical and practical implications and suggests future research directions. The authors also propose an abstruse model for HRM practices during M&A process for further investigation.Originality/valueThis is the first bibliometric study to explore the vast extant literature in M&A research related to the role of HRM practices in the execution of successful M&As.
Study on CO2 transport in fractal porous media for a Hausdorff fractal derivative advection-dispersion model
Authors: Chang Ailian , Huang Le , Li Qian-Qian , Vafai Kambiz , Shao Minglu 
Published: June 2023

Purpose
The classical advection-dispersion equation (ADE) model cannot accurately depict the gas transport process in natural geological formations. This paper aims to study the behavior of CO2 transport in fractal porous media by using an effective Hausdorff fractal derivative advection-dispersion equation (HFDADE) model.

Design/methodology/approach
Anomalous dispersion behaviors of CO2 transport are effectively characterized by the investigation of time and space Hausdorff derivatives on non-Euclidean fractal metrics. The numerical simulation has been performed with different Hausdorff fractal dimensions to reveal characteristics of the developed fractal ADE in fractal porous media. Numerical experiments focus on the influence of the time and space fractal dimensions on flow velocity and dispersion coefficient.

Findings
The physical mechanisms of parameters in the Hausdorff fractal derivative model are analyzed clearly. Numerical results demonstrate that the proposed model can well fit the history of gas production data and it can be a powerful technique for depicting the early arrival and long-tailed phenomenon by incorporating a fractal dimension.

Originality/value
To the best of the authors’ knowledge, first time these results are presented.
Pre-seed grant as an enabler of learning
Authors: Fauchald Ragnhild Nordeng , Aaboen Lise , Haneberg Dag Håkon 
Published: June 2023

PurposeThe paper focuses on how student entrepreneurs learn from the process of applying for low-threshold seed capital grants of about €2500Design/methodology/approachAn in-depth inductive study was conducted on the seed capital grant initiative TrønderEnergi–Bidraget (TEB). The research design was based on the Zaltman metaphor elicitation technique (ZMET) to capture the interviewees' perceptions about TEB. From the interviews, 596 codes were identified and grouped into 54 categories. The results are illustrated in a consensus map.FindingsTEB is an enabler of student venture creation processes through both the money awarded and activities fostering learning and development. Learning by doing is visible through two processes: 1) repeated writing of applications and 2) “forced” reflective thinking through the steps in the application process. The iterativeness of these processes due to repeated applications to the low threshold initiative is important for learning.Practical implicationsThe authors recommend that university managers and policymakers offer seed funding to student entrepreneurs to ensure that the offering is a low threshold. A low threshold is decisive for generating a positive learning outcome from the application process. The seed funding initiatives should require students to put time and energy into all the integrated processes to make value out of the iterativeness of the processes.Originality/valueThis paper extends the discussion on the additionality of receiving grants by focusing on the process of applying for a grant. This research contributes to the student entrepreneurship literature by suggesting that the design of the application process and forced reflections are important for learning, as well as specifying the antecedents for student motivation for continued entrepreneurial activity in the application process.
Smart destination competitiveness: underscoring its impact on economic growth
Authors: Lasisi Taiwo Temitope , Odei Samuel Amponsah , Eluwole Kayode Kolawole 
Published: June 2023

PurposeThe current study is designed to investigate the factors that foster the framing of destination competitiveness and establish the factors that drive the contribution of tourism innovations to economic growth in smart tourism destinations.Design/methodology/approachA four-year panel data were extracted from the World Economic Forum's travel and tourism competitiveness index and data were analysed using Poisson Pseudo Maximum Likelihood regression model.FindingsThe findings demonstrate that both the enabling environment and airport infrastructure significantly affect tourism's impact on the economy of the selected smart European tourism destinations. Conversely, human resources and general infrastructure display a negative correlation with tourism's contribution to the economy. However, no data in the sample support the idea that tourism policies, government prioritization or readiness of tourism information and communication technologies impact tourism's contribution to the economy. Additionally, the marginal effects indicate that improving the enabling environment and airport infrastructure can generate additional benefits for the economy through tourism.Originality/valueThe uniqueness of this study is the integration of smart tourism destinations with the measure of destination competitiveness to provide an empirical bridge that links tourism competitiveness to economic growth.
Hormone replacement therapy did not increase risk of melanoma in Chinese female with menopausal and postmenopausal disorders: A population‐based retrospective cohort study in Taiwan
Authors: Ma Jun , Chen Yi‐Hsien , Chiang Chien‐Ping , Wang Wei‐Ming , Chung Chi‐Hsiang , Tsao Chang‐Huei , Weng Zi‐Xeng , Hung Chih‐Tsung , Chien Wu‐Chien 
Published: June 2023

AbstractHormone replacement therapy (HRT) is widely used to relieve symptoms of menopause with proven efficacy. However, there has been significant controversy surrounding the use of HRT because of its potential link with an increased risk of cancer, particularly female reproductive organ cancers. That HRT increases the risk of melanoma is also disputed, and several cohort studies have produced variable results. To delineate the association between HRT and melanoma in Taiwan, we conducted a population‐based retrospective cohort study on 14 291 patients who had received HRT and 57 164 population controls in Taiwan between 2000 and 2013. Multivariate odds ratios (ORs) were calculated utilizing conditional logistic regression. Overall, the use of HRT was not significantly correlated with a higher risk of developing melanoma in Taiwan (95% confidence interval 0.386–1.099; p = 0.341). The hazard ratio analysis of melanoma and different HRTs showed there was no significant association between melanoma and the use of oral or external estrogens alone, including conjugated estrogens, estradiol, and estriol. Estrogen plus progesterone combined therapy was associated with a lower risk of melanoma. Only one case of melanoma was observed among the 2880 patients in this subgroup.
Lymphoproliferative disorder risk after methotrexate treatment for rheumatoid arthritis
Authors: Tanaka Keisuke , Ichikawa Ayako , Umezawa Natsuka , Yamamoto Kouhei , Yoshifuji Kota , Okada Keigo , Nogami Ayako , Umezawa Yoshihiro , Nagao Toshikage , Sakashita Chizuko , Mori Takehiko , Tohda Shuji , Koike Ryuji , Yasuda Shinsuke , Yamamoto Masahide 
Published: June 2023

AbstractMethotrexate (MTX)‐associated lymphoproliferative disorder (MTX‐LPD) is a troublesome problem in patients receiving MTX for rheumatoid arthritis (RA). However, its incidence, prognosis, and risk factors remain unclear. In this retrospective study, we evaluated the actual incidence, prognostic impact, and risk factors of MTX‐LPD. Of the 986 patients with RA treated with MTX, 90 patients experienced 95 new malignancies (NMs), with LPD as the most frequent in 26 patients. The cumulative LPD incidences were 1.3% and 4.7% at 5 and 10 years after MTX initiation, respectively. Among the 24 patients who discontinued MTX after developing LPD, 15 showed sustained regression, without difference in overall survival between patients with LPD and without NM. Inflammatory markers and absolute lymphocyte counts were not useful for early LPD development detection, but most of the patients with LPD had persistently elevated erythrocyte sedimentation ratios. Regarding concomitant drugs, tacrolimus increased the risk only if patients were not receiving biological disease‐modifying antirheumatic drugs (bDMARDs). bDMARDs did not increase the risk for any of the drugs or the number of classes used. The number of LPD cases was lower in patients with IL‐6A even after a long period after MTX, although with no statistically significant difference. Thus, approximately 1 in 20 patients with RA developed MTX‐LPD over the 10 years of MTX treatment, but it did not affect the survival of patients with RA. Tacrolimus increased the risk of developing LPD for certain patients and should be used with caution.
Visual Gaze Labeling for Augmented Reality Studies
Authors: Öney S. , Pathmanathan N. , Becher M. , Sedlmair M. , Weiskopf D. , Kurzhals K. 
Published: June 2023

AbstractAugmented Reality (AR) provides new ways for situated visualization and human‐computer interaction in physical environments. Current evaluation procedures for AR applications rely primarily on questionnaires and interviews, providing qualitative means to assess usability and task solution strategies. Eye tracking extends these existing evaluation methodologies by providing indicators for visual attention to virtual and real elements in the environment. However, the analysis of viewing behavior, especially the comparison of multiple participants, is difficult to achieve in AR. Specifically, the definition of areas of interest (AOIs), which is often a prerequisite for such analysis, is cumbersome and tedious with existing approaches. To address this issue, we present a new visualization approach to define AOIs, label fixations, and investigate the resulting annotated scanpaths. Our approach utilizes automatic annotation of gaze on virtual objects and an image‐based approach that also considers spatial context for the manual annotation of objects in the real world. Our results show, that with our approach, eye tracking data from AR scenes can be annotated and analyzed flexibly with respect to data aspects and annotation strategies.
Evaluating the safety and efficacy of aminolevulinic acid 20% topical solution activated by pulsed dye laser and blue light in the treatment of facial cutaneous squamous cell carcinoma in situ
Authors: Nestor Mark S. , Han Haowei , Ceci Francesca M. , Lawson Alec , Gade Anita 
Published: June 2023

AbstractBackgroundSquamous cell carcinoma (SCC) is the second most common cutaneous malignancy, after basal cell carcinoma (BCC). Photodynamic therapy (PDT) involves converting a photosensitizer to reactive oxygen intermediates, which preferentially bind to hyperproliferative tissue. The most commonly used photosensitizers are methyl aminolevulinate and aminolevulinic acid (ALA). Presently, ALA‐PDT is approved in the US and Canada for the treatment of actinic keratoses on the face, scalp, and upper extremities.AimsThis cohort study evaluated the safety, tolerability, and efficacy of aminolevulinic acid, pulsed dye laser, and photodynamic therapy (ALA‐PDL‐PDT) for treatment of facial cutaneous squamous cell carcinoma in situ (isSCC).MethodsTwenty adult patients with biopsy‐confirmed isSCC on the face were recruited. Only lesions 0.4–1.3 cm in diameter were included. Patients underwent two treatments with ALA‐PDL‐PDT spaced 30 days apart. The isSCC lesion was then excised 4–6 weeks following the second treatment for histopathological assessment.ResultsNo residual isSCC was detected in 17/20 (85%) patients. Two of the patients with residual isSCC had skip lesions present that explained the treatment failure. Excluding the patients with skip lesions, the posttreatment histological clearance rate was 17/18 (94%). Minimal side effects were reported.LimitationsOur study was limited by small sample size and lack of long‐term recurrence data.ConclusionsThe ALA‐PDL‐PDT protocol is a safe and well‐tolerated treatment option for isSCC on the face, providing excellent cosmetic and functional results.
The effects of weight management on heart failure: A systematic review and meta‐analysis of randomized controlled trials
Authors: Zhao Qiuge , Zhang Yilin , Dong Xiaoyu , Zhang Xiuting , Fan Xiuzhen 
Published: June 2023

AbstractBackgroundWeight management is an important part of disease management in patients with heart failure. However, the effectiveness of reported weight management interventions is inconclusive.AimsThe aim of this systematic review and meta‐analysis was to assess the effects of weight management on functional status, heart failure‐related hospitalizations, and all‐cause mortality in patients with heart failure.MethodsPubMed, Web of Science, Embase, and the Cochrane Library were searched on April 3, 2022. This study was registered with PROSPERO (CRD42021283817). Eligible studies assessed functional status, heart failure‐related hospitalizations, and all‐cause mortality in patients with heart failure. Two researchers independently screened the articles, extracted data, and evaluated the risk bias of each study. Dichotomous variables were presented as OR with a 95% confidence interval (CI). The data were analyzed using a fixed effect or random effect model, and heterogeneity was determined using I2 statistics. All statistical analyses were conducted using RevMan 5.3.ResultsAmong 4279 studies screened, seven randomized controlled trials were included in this study. The results showed that weight management significantly improved functional status (OR = 0.15, 95% CI [0.07, 0.35], I2 = 52%) and reduced the risk of all‐cause mortality (OR = 0.54, 95% CI [0.34, 0.85], I2 = 0%), but had no significant effect on heart failure‐related hospitalizations (OR = 0.72, 95% CI [0.20, 2.66]).Linking Evidence to ActionWeight management has effects on improved functional status and reduced all‐cause mortality in patients with heart failure. It is necessary to strengthen the weight management interventions of patients with heart failure to improve patients' functional status and reduce all‐cause mortality.
Supplemental Material: Controls on metal fertility of dioritic intrusions in the Laiwu district, North China Craton: Insights from whole-rock geochemistry and mineral compositions
Authors: Li Si-Yuan , Li Jian-Wei , et al.  
Published: June 2023

<p>Table S1: Major- and trace-element geochemistry of whole-rock samples from the Fe-mineralized and barren dioritic intrusions in the Laiwu district. Table S2: Sr-Nd isotopic compositions of whole-rock samples from the Fe-mineralized and barren plutons in the Laiwu district. Table S3: Major- and trace-element compositions of amphibole from the Fe-mineralized and barren plutons in the Laiwu district. Table S4: U-Pb age data and trace elements of zircon from the Fe-mineralized and barren plutons in the Laiwu district. Table S5: Major and trace elements of apatite from the Fe-mineralized and barren plutons in the Laiwu district. Figure S1: Hand specimens of diorites collected from the Kuangshan, Jiaoyu, Jinniushan, and Tietonggou plutons. Figure S2: Cathodoluminescence (CL) images of representative zircons from the Kuangshan, Jiaoyu, Jinniushan, and Tietonggou diorites. Figure S3: U-Pb concordia diagrams of zircons from the Kuangshan, Jiaoyu, Jinniushan, and Tietonggou plutons. Figure S4: Selected oxide element Harker’s plots for the Fe-mineralized and barren plutons in the Laiwu district. </p>
What is the impact of high-profile end-of-life disputes on paediatric intensive care trainees?
Authors: Bell Clare Emily , Dittborn Mariana , Brierley Joe 
Published: June 2023

IntroductionThis study explores UK paediatric intensive care (PIC) trainees’ thoughts and feelings about high-profile end-of-life cases recently featured in the press and social media and the impact on their career intentions.MethodsSemi-structured interviews were conducted with nine PIC-GRID trainees (April to August 2021). Interview transcripts were analysed using thematic analysis.ResultsSix main themes were identified: (1) All participants wished to do what was best for the child, feeling conflicted if this meant disagreeing with parents. (2) Interviewees felt unprepared and expressed deep concern about the effect of high-profile cases on their future career; all had reconsidered their training in PIC due to concerns about future high-profile end-of-life disputes, despite this all were still in training. (3) Specific training on the ethical and legal nuances of such cases is required, alongside targeted communication skills. (4).All cases are unique. (5) All had purposefully minimised their social media presence. (6) Working in a supportive environment is crucial, underscoring the importance of clear and unified team communication.ConclusionUK PIC trainees feel unprepared and anxious about future high-profile cases. A parallel can be drawn to child protection improvements following significant educational investment after government reports into preventable child abuse deaths. Models for supporting trainees and establishing formal PIC training are required to improve trainees’ confidence and skills in managing high-profile cases. Further research with other professional groups, the families involved and other stakeholders would provide a more rounded picture.
Barriers and facilitators to implementation of point-of-care lung ultrasonography in a tertiary centre in Benin: a qualitative study among general physicians and pneumologists
Authors: Suttels Veronique , Guedes Da Costa Sofia , Garcia Elena , Brahier Thomas , Hartley Mary-Anne , Agodokpessi Gildas , Wachinou Prudence , Fasseur Fabienne , Boillat-Blanco Noémie 
Published: June 2023

ObjectivesOwing to its ease-of-use and excellent diagnostic performance for the assessment of respiratory symptoms, point-of-care lung ultrasound (POC-LUS) has emerged as an attractive skill in resource-low settings, where limited access to specialist care and inconsistent radiology services erode health equity.To narrow down the research to practice gap, this study aims to gain in-depth insights in the perceptions on POC-LUS and computer-assisted POC-LUS for the diagnosis of lower respiratory tract infections (LRTIs) in a low-income and middle-income country (LMIC) of sub-Saharan Africa.Design and settingQualitative study using face-to-face semi-structured interviews with three pneumologists and five general physicians in a tertiary centre for pneumology and tuberculosis in Benin, West Africa. The center hosts a prospective cohort study on the diagnostic performance of POC-LUS for LRTI. In this context, all participants started a POC-LUS training programme 6 months before the current study. Transcripts were coded by the interviewer, checked for intercoder reliability by an independent psychologist, compared and thematically summarised according to grounded theory methods.ResultsVarious barriers− and facilitators+ to POC-LUS implementation were identified related to four principal categories: (1) hospital setting (eg, lack of resources for device renewal or maintenance−, need for POC tests+), (2) physician’s perceptions (eg, lack of opportunity to practice−, willingness to appropriate the technique+), (3) tool characteristics (eg, unclear lifespan−, expedited diagnosis+) and (4) patient’s experience (no analogous image to keep−, reduction in costs+). Furthermore, all interviewees had positive attitudes towards computer-assisted POC-LUS.ConclusionsThere is a clear need for POC affordable lung imaging techniques in LMIC and physicians are willing to implement POC-LUS to optimise the diagnostic approach of LRTI with an affordable tool. Successful integration of POC-LUS into clinical routine will require adequate responses to local challenges related to the lack of available maintenance resources and limited opportunity to supervised practice for physicians.