bluuebunny/crossref_metadata_2025_split huggingface dataset:
Scientific creativity and innovation ability and its determinants among medical postgraduate students in Fujian province of China, a cross sectional study.
Authors: Liu Fengqiong , Qu Shuming , Fan Yi , Chen Fa , Hu Zhijian , He Baochang 
Published: January 2023

Abstract
Background: Graduate education is a main approach to train high level innovative elites. With the expansion of the scale of graduate education in China, it has gradually emerged that the lack of innovation ability of graduate students is recognized as the primary problem in graduate education. How to comprehensively improve the quality of postgraduate teaching has become the core task of educational reform and development. However, data about the current cultivation and development of innovative ability of graduate students in China is limited.
Methods: A questionnaire survey was conducted among medical postgraduate students. Descriptive statistics and multiple regression analysis methods were used to analyze the data to describe the current innovation ability in advanced medical education and potential influence factors.
Results: A total of 1241 medical students were surveyed, according to the results of questionnaire data analysis. The proportion of subjects who participated in the College Student’s Entrepreneurship and Innovation program or any other scientific research programs are fairly high which are 46.82% and 29.20% respectively. Most of participant are observed with high levels of self motivation and active learning, and have good performance in creative thinking. However, only a small number of participants (16.6%) reported academic achievements such as publications. Most of the students are satisfied with the current scientific research environment and think that the current postgradute training system is qualified for cultivation of innovation ability, and expects the inclusion of course specialized in systemic medicine and medical informatics in the curricula. Multiple logistic regression results showed that among the factors studied, gender, medical specialties, types of master degree are associated with cognition & skills, academic performance and creativity.
Conclusions: It will be important to incorporate more techniques of creating and improving creativity in the curricula of the current postgraduate education, especially for course such as systemic medicine and informatics. Guidance in the earlier school life can stimulate creativity and an early introduction into scientific research work will facilitate innovative thinking and behavior. Scientific research programs such as the National Innovation and Entrepreneurship Training for University of PRC have been widely implemented in the undergraduate education system throughout the country. However, training effectiveness of the current scientific research programs is worth improving.
Mechanisms of ferroptosis with immune infiltration and inflammatory response in rotator cuff injury
Authors: Tong Zhicheng , Li Huimin , Jin Yanglei , Sheng Lingchao , Ying Mingshuai , Liu Qixue , Wang Chenhuan , Teng Chong 
Published: January 2023

Abstract
Background
Rotator cuff (RC) tears are one of the most common shoulder disorders. The causes of RC tears include acute trauma and chronic impact injury. The clinical outcome of RC tears is not satisfactory because the pathogenesis of RC tears is not well understood. Therefore, the molecular mechanisms involved in RC tears need to be elucidated in order to develop clinical innovative therapies.
Methods
The mechanism of ferroptosis and inflammation involved in the development of RC tears was investigated and confirmed using a combination of bioinformatics methodology and in vivo experimental approaches.
Results
The Gene Expression Omnibus database was used to obtain the microarray data relevant to the RC tears for further investigation. In addition to carrying out function enrichment analysis, protein-protein correlation analysis, building the correlation regulatory network, and examining potential associations between ferroptosis and immune abnormalities and inflammatory responses in RC tears, a number of bioinformatic techniques were used to identify the ferroptosis-related genes. Last but not least, we created an RC tears rat model for in vivo experimental validation. This model was primarily based on histological analysis and the qPCR approach to assess the major correlation pairs in regulating ferroptosis and the inflammatory response in RC tears. For the additional function enrichment analysis, 10 hub ferroptosis-related genes were chosen to construct the correlation regulation network. In RC tears, it was discovered that genes related to hub ferroptosis and hub inflammatory response were strongly correlated. The outcomes of in vivo tests showed that RC tears were related to Cd68-Cxcl13, Acsl4-Sat1, Acsl3-Eno3, Acsl3-Ccr7, and Ccr7-Eno3 pairings in regulating ferroptosis and inflammatory response.
Conclusion
The pairs Cd68-Cxcl13, Acsl4-Sat1, Acsl3-Eno3, Acsl3-Ccr7, and Ccr7-Eno3 were found to be connected to RC tears in regulating ferroptosis and the inflammatory response. Our results show an association between ferroptosis and inflammation, providing a new avenue to explore the clinical treatment of RC tears.
Characterization and identification of filamentous bacterium XJ-16 and analysis of its bioactive components
Authors: Jiang Ke , Bai Ruoxuan , Luo Xianglian , Gao Ting , Xu Fangxu , Zhao Hongxin , Zhang Dianpeng 
Published: January 2023

Abstract
Actinomycetes, which can produce a variety of bioactive compounds in the metabolic process, is one of the important sources of novel drugs, enzymes, anti-tumor drugs and enzyme inhibitors. It has been the focus of researchers to find and develop Actinomycetes with special characters. Strain XJ-16 is a blue alkali-resistant filamentous bacterium with high antimicrobial activity isolated from saline-alkali land of Xinjiang. Based on the classification, the enzyme production, metabolite antibacterial activity, and antibacterial substance isolation of XJ-16 were explored. which showed that XJ-16 belongs to the blue group of Streptomyces sp, and it can secrete cellulase, lipase, urease, protease, catalase and oxidase during metabolism. In addition, the bacteriostatic substance secreted by the strain XJ-16 showed inhibitory effects against both Gram-positive and Gram-negative bacteria, as well as the yeast Candida albicans. Then it was found that the bacteriostasis produced by XJ-16 has strong tolerance to acid, weak tolerance to alkali, and easy to be inactivated. After tested by HPLC, the retention time of antimicrobial substance was 13.261 min. This study provides a theoretical basis for the search for new antibacterial compounds and the further development of blue alkaline Actinomycetes XJ-16.
Adsorption of Cd (II) by a novel living and non-living Cupriavidus necator GX_5: optimization, equilibrium and kinetic Studies
Authors: Li Xingjie , Xiao Qiusheng , Shao Qin , Li Xiaopeng , Kong Jiejie , Liu Liyan , Zhao Zhigang , Li Rungen 
Published: January 2023

Abstract
Biosorbents have been extensively studied for heavy metal adsorption due to their advantages of low cost and high efficiency. In the study, the living and non-living biomass of Cupriavidus necator GX_5 previously isolated were evaluated for their adsorption capacity and/or removal efficiency for Cd (II) through batch experiments, SEM and FT-IR investigations. The maximum removal efficiency rates for the live and dead biomass were 60.51% and 78.53%, respectively, at an optimum pH of 6, a dosage of 1 g/L and an initial Cd (II) concentration of 5 mg/L. The pseudo-second-order kinetic model was more suitable for fitting the experimental data, indicating that the rate-limiting step might be chemisorption. The Freundlich isotherm model fit better than the Langmuir isotherm model, implying that the adsorption process of both biosorbents was heterogeneous. FT-IR observation reflected that various functional groups were involved in Cd (II) adsorption: -OH, -NH, C=O, C-O and C-C groups for the living biomass and -OH, -NH, C-H, C=O, C-N and N-H groups for the dead biomass. Our results imply that non-living biosorbents have a higher capacity and stronger strength for absorbing Cd (II) than living biomass. Therefore, we suggest that dead GX_5 is a promising adsorbent and can be used in Cd (II)-contaminated environments.
Speed and quality of interbody fusion in porous bioceramic Al 2 O 3 and polyetheretherketone cages for anterior cervical discectomy and fusion: a comparative study
Authors: Kostysyn Roman , Ryska Pavel , Jandura Jiri , Selke-Krulichova Iva , Poczos Pavel , Hosszu Tomas , Cesak Tomas 
Published: January 2023

Abstract
Background The objective of this prospective randomized monocentric study is to compare the speed and quality of interbody fusion of implanted porous Al2O3 (aluminium oxide) cages with PEEK (polyetheretherketone) cages in ACDF (anterior cervical discectomy and fusion).
Materials and methods A total of 111 patients were enrolled in the study, which was carried out between 2015 and 2021. The 18-month follow-up (FU) was completed in 68 patients with an Al2O3 cage and 35 patients with a PEEK cage in one-level ACDF. Initially, the first evidence (initialization) of fusion was evaluated on computed tomography (CT). Subsequently, interbody fusion was evaluated according to the fusion quality scale, fusion rate and incidence of subsidence.
Results Signs of incipient fusion at 3 months were detected in 22% of cases with the Al2O3 cage and 37.1% with the PEEK cage. At 12-month FU, the fusion rate was 88.2% for Al2O3 and 97.1% for PEEK cages, and at the final FU at 18 months, 92.6% and 100%, respectively. The incidence of subsidence was observed to be 11.8% and 22.9% of cases with Al2O3 and PEEK cages, respectively.
Conclusions Porous Al2O3 cages demonstrated a lower speed and quality of fusion in comparison with PEEK cages. However, the fusion rate of Al2O3 cages was within the range of published results for various cages. The incidence of subsidence of Al2O3 cages was lower compared to published results. We consider the porous Al2O3 cage as safe for a stand-alone disc replacement in ACDF.
Genome-Wide Identification and Analysis of the Cryptochrome/Photolyase Family in brown algae Saccharina japonica
Authors: Wu Yukun , Zhang Pengyan , Liang Zhourui , Yuan Yanmin , Liu Yi , Zhang Di , Liu Fuli 
Published: January 2023

Abstract
Cryptochrome/Photolyase Family (CPF) is a widely distributed and highly conserved gene family. The CPF includes photolyases that repair UV-induced DNA damage and blue-light receptors cryptochromes that are known for their photoreceptive functions in terrestrial plants. This study mainly emphasized the CPF members of the economically important brown alga Saccharina japonica, and traces the evolutionary relationship and distribution of this family in algae. Eight new CPF members were identified from the genomes of S. japonica and Undaria pinnatifida, and sixty CPF members from other different species were collected simultaneously for the phylogenetic analysis. Phylogenetic and transcriptional analyses provide evidence for the evolution and function of CPF genes in brown algae. According to the phylogenetic tree, the CPF family was divided into five branches. Photolyase homology domain (PHR) and a non-covalently bound flavin adenine dinucleotide (FAD) molecule are present in the CPF members of two brown algae S. japonica and U. pinnatifida. But they lack the typical C-terminal extension (CCT) characteristic of plant-like cryptochromes. The brown algae Ectocarpus siliculosus and U. pinnatifida have both animal-like CPF and plant-like CPF while only plant CPF members were identified in S. japonica. The results of RNA-seq and qPCR indicated that Sj Cryptochrome-DASH1 (CRY-DASH1) and Sj cyclobutane pyrimidine dimer (CPD) genes may not only play a role in light response but also play an important role in the gametophyte development of S. japonica. Protein interaction network of Sj CPF members showed that there are circadian clock protein Timeless homologous genes in S. japonica, which are located in the core position of the protein interaction network. This implied that Sj CPF members may transmit information by interacting with downstream Timeless genes. These results provide information for subsequent research on functional and regulatory pathways of CPFs in algae species.
SAFE AI models to measure the impact of ESG factors on credit ratings
Authors: Agosto Arianna , Cerchiello Paola , Giudici Paolo 
Published: January 2023

Abstract
Artificial Intelligence methods, based on machine learning from data, are rapidly changing financial services, and credit lending in particular, complementing bank lending with platform lending.While financial technologies improve user experience, and possibly lower costs, they may increase risks and, in particular, the model risks that derive from inaccurate credit rating assessments.In this paper we will show how to reduce model risks, using a S.A.F.E. statistical learning model, that is: taking environmental, social and governance factors into account, to improve Sustainability, building a model for which ESG factors do predict credit ratings, to improve their Accuracy, dealing with data inconsistencies, to improve Fairness; merging ESG scores into one mixture, to improve Explainability.
Evaluation of plasma CD36 and glutathione as potential biomarkers for intracranial aneurysm.
Authors: Wang Hanbin , Wang Luxuan , Liu Yunmei , Men Weidong , Hao Wanjiao , Fang Chuan , Li Chunhui , Zhang Lijian 
Published: January 2023

Abstract
The underlying mechanisms of intracranial aneurysm (IA) formation and rupture are still unclear. Evidence has proved that it might be closely related to inflammatory response and oxidative stress. Our objective was to identify novel inflammatory and oxidative stress related biomarkers to assist IA management. In this study, the enzyme-linked immunosorbent assay was performed to measure the expression levels of CD36 and glutathione (GSH) in the plasma of 30 IA patients and 30 healthy controls. Then, correlation analysis and receiver operating characteristic (ROC) curve, and logistic regression analysis were applied to investigate CD36 and GSH as potential biomarker for IA. The expression level of plasma CD36 in the IA patients was significantly higher than that in the control group (P < 0.0001), and the level of plasma GSH in the IA patients was significantly lower than that in the control group (P < 0.0001). The plasma level of CD36 and GSH did not show significant correlation with age, Glasgow Coma Scale (GCS), Hunter-Hess score, aneurysm size, aneurysm height, aneurysm neck, and aspect ratio. ROC analysis showed that CD36 and GSH had high sensitivity (90.0%, 96.6%) and specificity (96.6%, 86.6%) for IA diagnosis. And the combined sensitivity and specificity achieved 100% and 100%, respectively. The AUC of logistic regression model based on CD36 and GSH was 0.505. Our results suggested that CD36 and GSH might participate in the process of IA formation and rupture but did not affect its morphology. Moreover, the combination plasma CD36 and GSH could serve as potential biomarker for IA rupture.
A survey of surgically resected pituitary incidentalomas and a comparison of the clinical features and surgical outcomes of non-functioning pituitary adenomas discovered incidentally versus symptomatically
Authors: Guerreiro Vanessa , Mendonça Fernando , Freitas Paula , pereira Josué , Bernanrdes Irene , Pinheiro Jorge , Guimarães Tiago , Carvalho Davide 
Published: January 2023

Abstract
Background
Clinically nonfunctioning pituitary adenomas(CNFPAs) constitute one of the most common tumors in the sellar region and are often discovered only when associated with compressive symptoms. With the frequent use of brain imaging, there has been an increase in the prevalence of incidentally discovered CNFPAs.
Aim
We aim to determine the prevalence of incidental diagnosis with CNPAs observed over a decade and compare the analytical, clinical and treatment differences between those who were diagnosed either incidentally or symptomatically. We also intend to evaluate the pathology differences between both groups.
Methods
We retrospectively analyzed patients aged ≥ 18years with an apparent CNFPA.defined as a pituitary lesion compatible with pituitary adenoma which is not associated with the clinical or biochemical evidence of a hormone-secreting tumor. Inclusion criteria included normal prolactin level for lesions < 9mm or a prolactin level < 100ng/mL for lesions ≥ 10mm in maximal tumor diameter.
Results
We included 119patients [53.8% males; mean age:56.8 years(SD = 16.7)]. Diagnosis was incidental in 47.1% of patients and many patients had unappreciated signs and symptoms of pituitary disease. In the symptomatic and incidental groups,66.7% and41.1% of patients had hypopituitarism, respectively(p = 0.005). Only20.4% of patients incidentally diagnosed had microadenoma(p = 0.060). Hypopituitarism was present in18.8% of those patients with microadenomas. Most tumors were macroadenomas(87.4%). Half of those patients diagnosed incidentally were submitted to surgery, compared to75.8% of those who were diagnosed symptomatically(p = 0.004).
Conclusions
CNFPAs are commonly diagnosed incidentally, with many manifesting symptoms on examination. Accordingly, if there was a greater level of knowledge and more suspicion about these pathologies, it might be possible to discover them earlier.
P-15 promotes chondrocyte proliferation in osteoarthritis by regulating SFPQ to target the Akt-RUNX2 axis
Authors: Li Yuanli , Nie Junlan , Deng Changgong , Li Hong 
Published: January 2023

Abstract
Background
The disruption of chondrocyte proliferation and differentiation is a critical event during the process of joint injury in osteoarthritis (OA). P-15 peptides could bind to integrin receptors on various precursor cells, promote cell adhesion, release growth factors, and promote the differentiation of osteoblast precursor cells. However, the role it plays in OA, particularly in chondrocyte proliferation, is not fully understood.
Methods
The activity of SFPQ and RUNX2 in the bone tissue of patients with osteoarthritis was analyzed using quantitative real-time polymerase chain reaction (qRT-PCR). Interleukin-1β (IL-1β) inducer was performed to establish an in vitro model of OA. Cell proliferation was measured by CCK-8 assay. The expressions of COL2a1, ACAN, COMP, SOX9, and BMP2 related to cartilage differentiation were detected using qRT-PCR. In addition, the expression levels of SFPQ, AKT, p-AKT, and RUNX2 were detected using Western blotting.
Results
The results showed that the expression of SFPQ was significantly decreased and the expression of RUNX2 was significantly increased in osteoarthritis cartilage tissue. P-15 peptide reversed IL-1β-induced cell proliferation obstruction and alleviated chondrocyte damage. Furthermore, P-15 polypeptide increased the expression levels of cartilage differentiation genes COL2a1, ACAN, and BMP2, while decreasing the expression of COMP and SOX9 in an inverse dose-dependent manner. Then specific interfering RNA proved that P-15 maintains chondrocyte stability and is associated with the SFPQ gene. Finally, we confirmed that P-15 inhibited the Akt-SUNX2 pathway, which is regulated in the expression of SFPQ.
Conclusions
P-15 can mitigate chondrocyte damage and osteoarthritis progression by inhibiting cell death and modulating SFPQ-Akt-SUNX2 pathway, offering an opportunity to develop new strategies for the treatment of osteoarthritis.
Associations of whole blood zinc levels with coronary artery calcification and future cardiovascular events in CKD patients
Authors: Zhang Danfeng , Zhu Yuyu , Li Huan , Wang Yunfei , Niu Zheng , Zhou Wenli , Wang Deguang 
Published: January 2023

Abstract
This study was conducted to compare the differences of the whole blood zinc concentration in patients with chronic kidney disease (CKD) as compared to healthy controls, and to explore the correlations of the whole blood zinc level with coronary artery calcification (CAC) and cardiovascular event (CVE) in CKD patients. 170 CKD patients and 62 healthy controls were recruited . The whole blood zinc concentration were determined in using atomic absorption spectroscopy (AAS) method. The degrees of CAC were evaluated by Agatston score based on computed tomography (CT). Regular follow-up visits were performed to record the incidence of CVE, and risk factors were analyzed by COX proportional hazard model and Kaplan-Meier survival curve. There were statistically significant lower zinc in CKD patients than in healthy population. The prevalence of CAC was 58.82% in CKD patients. Our study found that dialysis duration, iPTH, ALP, 25(OH)D3, neutrophil-lymphocyte ratio (NLR), total cholesterol and Hs-CRP were positively correlated with CAC, while albumin, Hb and zinc were negatively correlated with CAC. A COX proportional hazard model demonstrated that moderate to severe CAC, NLR, phosphate, 25(OH)D3, iPTH and HLDL were associated with an increased risk for CVE, while zinc, Hb and albumin was inversely associated with a reduced risk for CVEs. Kaplan-Meier curve showed that low zinc (zinc <86.62μmol/L) patients and moderate to severe CAC patients had lower survival respectively. Our study found the lower levels of zinc and higher prevalence of CAC in CKD patients, the low zinc is involved in the high incidence rate of moderate to severe CAC and CVE in CKD patients.
Overexpression of PTEN gene increases Ins2 gene mRNA expression, not Ins1 gene mRNA expression, in insulinoma cell line Rin-5F
Authors: Kiba Takayoshi 
Published: January 2023

Abstract
One functional neuroendocrine tumor that causes hypoglycemia due to insufficiently high insulin production is an insulinoma. In rats, two genes coding for insulin, insulin 1 (Ins1) and insulin 2 (Ins2) are found on chromosome 1. Ins1 was produced from an Ins2 transcript, and it was inserted into the genome via an RNA-mediated duplication-transposition event, according to some structural feature analyses. In this study, the author has looked at how overexpression of the PTEN gene in the insulinoma cell line Rin-5F affects the expression of the insulin genes, Ins 1 and Ins 2. In the insulinoma cell line, overexpression of the PTEN gene boosts Ins2 gene mRNA expression but not Ins1 gene mRNA expression. It has been reported that PTEN upregulates insulin signaling by increasing insulin receptor substrate (IRS)-2 mRNA levels. Also, PTEN has been reported to be secreted in exosomes and thereafter, into extracellular space. The present study has suggested that overexpression of PTEN might induce the increasing Ins 2 gene expression, one of the phosphorylated genes against the IRS-2 through the insulin/IGF-1 receptor. This can suggest further investigation and examination of the PTEN gene expression and insulinoma carcinogenesis.