bluuebunny/crossref_metadata_2025_split huggingface dataset:
The mechanism of flavonoids from Cyclocarya paliurus on inhibiting liver cancer based on in vitro experiments and network pharmacology
Authors: Mo Jinggang , Tong Yingpeng , Ma Junxia , Wang Kunpeng , Feng Yifu , Wang Liezhi , Jiang Hao , Jin Chong , Li Junmin 
Published: February 2023

Introduction:Cyclocarya paliurus (Batal.) Iljinsk., a subtropical tree belonging to the family Juglandaceae, is rich in polysaccharides, flavonoids, and terpenoids. It has important pharmacological effects such as lowering blood lipids, blood sugar, and blood pressure. However, little has been discerned regarding anti tumor effects and their potential mechanisms.Method:In vitro cell culture experiments were used to test the effect of C. paliurus total flavonoids (CTFs) extract on apoptosis mechanisms in HepG2 cells. Network pharmacology was applied to further explore the effects of CTFs on liver cancer as well as the mechanisms through which these effects might be achieved. Both 3 hydroxyflavone and luteolin were randomly selected to verify the effect on inducing apoptosis and inhibiting the proliferation of HepG2 cells.Results and Discussion: Network pharmacological analysis was applied to these 62 compounds and their targets, and 13 flavonoids were further screened for their potential anti liver cancer activity. These 13 flavonoids included: tangeretin, baicalein, 7,3′-dihydroxyflavone, velutin, 3-hydroxyflavone, chrysin, kumatakenin, tricin, luteolin, chrysoeriol, apigenin, pinocembrin, and butin. Together, these flavonoids were predicted to interact with AKT1, MAPK3, PIK3CA, EGFR, MAP2K1, SRC, IGF1R, IKBKB, MET, and MAPK14. It was predicted that the inhibitory effect on hepatocellular carcinoma would be accomplished by regulation of core proteins relating to such KEGG pathways as cancer, PI3K-Akt, proteoglycans in cancer, microRNAs in cancer, and endocrine resistance via core target proteins. Both 3-hydroxyflavone and luteolin were demonstrated to induce apoptosis and inhibit the proliferation of HepG2 cells. Our study provides scientific evidence supporting the use of CTFs for the treatment of liver cancer.
The protective roles of allicin on type 1 diabetes mellitus through AMPK/mTOR mediated autophagy pathway
Authors: Qian Rengcheng , Chen Huihui , Lin Hongzhou , Jiang Yalan , He Pingping , Ding Yinjuan , Wu Huilan , Peng Yongmiao , Wang Lingfei , Chen Congde , Wang Dexuan , Ji Weiping , Guo Xiaoling , Shan Xiaoou 
Published: February 2023

Background: Type 1 diabetes mellitus (T1DM) is one of the most common endocrine and metabolic diseases in children. Pancreatic β cells are thought to be critical cells involved in the progression of T1DM, and their injury would directly lead to impaired insulin secretion.Purpose: To investigate the protective effects of allicin on pancreatic β cell injury and elucidate the underlying mechanism.Methods: The streptozotocin (STZ)-induced mouse T1DM model in vivo and STZ-induced pancreatic β cell Min6 model in vitro were used to explore the effects of allicin on T1DM. The experiments include fasting blood glucose test, oral glucose tolerance detection, HE staining, immunohistochemistry, immunofluorescence, TUNEL staining, western blot, real-time quantitative PCR (RT-qPCR), and flow cytometry.Results: Allicin could significantly decrease blood glucose level, improve islet structure and insulin expression, and inhibit apoptosis to reduce STZ-induced pancreatic β cell injury and loss through activating AMPK/mTOR mediated autophagy pathway.Conclusion: Allicin treatment significantly reduced STZ-induced T1DM progression, suggesting that allicin may be a potential therapy option for T1DM patients.
Relative comparison of chronic kidney disease-mineral and bone disorder rat models
Authors: Zhang Xiaoqiong , Li Ting , Wang Lijuan , Li Yanhui , Ruan Taoren , Guo Xiaohong , Wang Qin , Meng Xianli 
Published: February 2023

Objective: The aim of this study is to establish a suitable animal model of chronic kidney disease–mineral and bone disorder (CKD–MBD) by comparing CKD–MBD rat models induced by 5/6 Nx, AN, and UUO, accompanied by a low-calcium and high-phosphorus diet.Methods: Sprague‒Dawley rats were randomly divided into four groups: control group, 5/6 nephrectomy (5/6 Nx) group, Adriamycin nephropathy (AN) group, and unilateral ureteral obstruction (UUO) group. Serum biochemical indices were measured to evaluate renal function, mineral and bone metabolism, the severity of CKD–MBD, and the status of bone transformation. Hematoxylin–eosin staining (HE) and Masson’s trichrome (Masson) staining were used for histopathological analysis of the kidney. Goldner’s trichrome (Goldner) and tartrate-resistant acid phosphatase (TRAP) staining were utilized to observe bone mineralization and osteoclasts in the femur, respectively. Micro-CT images were applied to study the structure of the femur. The expression levels of osterix and cathepsin K in the femur were measured by immunohistochemistry (IHC) to confirm the status of bone transformation.Results: The levels of serum creatinine (Scr) and blood urea nitrogen (BUN) in the 5/6 Nx and AN group rats were significantly higher than those in the control rats, and this change was accompanied by marked changes in the levels of calcium (Ca), phosphate (Pi), intact parathyroid hormone (i-PTH), fibroblast growth factor 23 (FGF23), osteocalcin (OC), and cross-linked C-telopeptide of type 1 collagen (CTX-1); UUO group rats exhibited slight and inconsistent variations in the levels of Scr, BUN, Ca, Pi, i-PTH, FGF23, OC, and CTX-1 in serum. Histopathological analysis of the kidney showed that the UUO group rats suffered serious fibrosis and 5/6 Nx group rats exhibited severe focal calcification. Histopathological analysis of the femur showed that the AN group rats had minimal bone mineralization and that the 5/6 Nx group rats had overactive osteoclasts. Micro-CT revealed that the AN model had the most severe bone destruction and that the 5/6 Nx model had the least severe bone loss among the three models. The expression of cathepsin K in the femur was significantly increased in all models, while the expression of osterix in the femur was only significantly increased in the 5/6 Nx model.Conclusion: 5/6 Nx, AN, and UUO accompanied by a low-calcium and high-phosphorus diet successfully induced CKD–MBD in rats. The 5/6 NX model presented the progression of high-turnover bone disease, with consistency between biochemical indices in serum and histomorphometric analysis of the femur, and the AN and UUO models developed a severe deterioration in bone quantity and severe bone resorption; however, the changes in biochemical indices were subtle in the UUO model, and liver injury was obvious in the AN model.
A meeting of positive behaviors: The relations of three aspects of flexibility with character strengths
Authors: Vylobkova Valentina , Heintz Sonja 
Published: February 2023

IntroductionThe adaptation of own plans and behaviors to new circumstances seems to be a desirable personal quality in the modern world. it has been assumed that adaptability cannot be transferred to a single character strength.MethodsThe present research examines this assumption using typical and daily behaviors of three aspects of flexibility (predictability, adaptability and orderliness) and 24 character strengths across two studies (N1 = 283, N2 = 188).ResultsFlexibility showed a consistent and large overlap with character strengths. Adaptability was positively related to most of the strengths. Predictability was positively related to humility and prudence, and orderliness to perseverance, prudence, and selfregulation.DiscussionThese results support our initial assumption and build a strong basis for further examining the relationships and interplay between flexibility and character strengths. They also constitute an important first step toward integrative positive interventions that target relevant aspects of flexibility and character.
Research on sports reform in the network era based on educational psychology in the context of the COVID-19
Authors: Fan QI 
Published: February 2023

IntroductionIn the context of the continuous progress of the “Internet+” era and the exceptionally severe outbreak of the Covid-19, online education confronts many challenges and also brings unlimited development opportunities.MethodsIn this paper, taking six universities with sports majors in Chongqing as the research objects, we analyze the current situation and problems of the development and construction of online education resources for sports in colleges and universities by browsing the official websites of colleges and universities, investigating the cooperation between colleges and universities and Massive Open Online Course (MOOC), and conducting a questionnaire survey on students.ResultsThe results show that it is necessary to innovate the traditional inefficient classroom teaching model based on the values of adapting to the characteristics of the “Internet+” era and learning advanced teaching rules, thus optimizing teaching effectiveness and fostering students’ independent learning, thinking and innovation.DiscussionWe investigated students’ online learning habits, their experience of using online education platform and the difficulties they encountered in the process, based on which two issues were raised: learning needs and learning experience.
Post-traumatic stress symptoms and burnout in healthcare professionals working in neonatal intensive care units: Results from the STRONG study
Authors: Ravaldi C. , Mosconi L. , Mannetti L. , Checconi M. , Bonaiuti R. , Ricca V. , Mosca F. , Dani C. , Vannacci A. 
Published: February 2023

BackgroundNewborns’ deaths and life-threatening conditions represent extremely stressful events for parents and professionals working in NICUs, facilitating the onset of secondary traumatic stress symptoms. The STRONG study aims to better understand the psychological impact on Italian NICUs staff of bereavement care.MethodsThe STRONG (STress afteR lOss in NeonatoloGy) study is a cross-sectional study based on a web survey consisted of four sections: sociodemographic, CommuniCARE-Newborn questionnaire, the Maslach Burnout Inventory and the Impact of Event Scale-Revised.Results227 NICU workers (42.7% nurses, 23.3% midwives, 22.2% physicians, 11.8% other HCPs) answered the survey. The hardest tasks were “communicating baby’s death” and “informing on autopsy results”; 44.7% of HCPs did not receive formal training in communicating bad news, 44.2% ‘learned from the field’ by watching other colleagues; 41.2% declared that they do not have any communication strategy. More than 90% of professionals thought that training on bereavement care is necessary. The majority of HCPs showed some degree of post-traumatic stress symptoms: 34% medium and 35.3% severe. Professionals with training in bereavement care and/or in communication had less probability to develop stress symptoms. A multivariate analysis showed that higher levels of burnout were associated with 4 or more monthly losses and medium or severe stress symptoms. Having a well-defined communication strategy for breaking bad news was independently associated with a better personal accomplishment.ConclusionDealing with newborns’ deaths is a highly stressful task; professionals should receive proper support such as debriefing, psychological support and training in order to prevent post-traumatic stress symptoms and reduce professional burnout.
SDG5 “Gender Equality” and the COVID-19 pandemic: A rapid assessment of health system responses in selected upper-middle and high-income countries
Authors: Kuhlmann Ellen , Lotta Gabriela , Fernandez Michelle , Herten-Crabb Asha , Mac Fehr Leonie , Maple Jaimie-Lee , Paina Ligia , Wenham Clare , Willis Karen 
Published: February 2023

IntroductionThe COVID-19 pandemic disrupted healthcare and societies, exacerbating existing inequalities for women and girls across every sphere. Our study explores health system responses to gender equality goals during the COVID-19 pandemic and inclusion in future policies.MethodsWe apply a qualitative comparative approach, drawing on secondary sources and expert information; the data was collected from March–July 2022. Australia, Brazil, Germany, the United Kingdom, and the USA were selected, reflecting upper-middle and high-income countries with established public health and gender policies but different types of healthcare systems and epidemiological and geo-political conditions. Three sub-goals of SDG5 were analyzed: maternity care/reproductive health, gender-based violence, and gender equality/women's leadership.ResultsWe found similar trends across countries. Pandemic policies strongly cut into women's health, constrained prevention and support services, and weakened reproductive rights, while essential maternity care services were kept open. Intersecting gender inequalities were reinforced, sexual violence increased and women's leadership was weak. All healthcare systems failed to protect women's health and essential public health targets. Yet there were relevant differences in the responses to increased violence and reproductive rights, ranging from some support measures in Australia to an abortion ban in the US.ConclusionsOur study highlights a need for revising pandemic policies through a feminist lens.
Improving functional outcomes and quality of life in an elderly woman with sarcopenia and spinal Tuberculosis: A case report
Authors: Defi Irma Ruslina , Hamam Nur Rusyidah , Biben Vitriana , Al Barqi Nuzula Chafidh 
Published: February 2023

BackgroundIndonesia is the world's second-largest contributor to TB patients. According to prevalence by age, the elderly have the most diagnosed TB. In Indonesia, TB spondylitis affects approximately 5% of TB cases and is a common cause of non-traumatic spinal cord injury (NTSCI). Spinal cord injury (SCI) is a growing public health concern, particularly among the elderly, as many of its populations have sarcopenia. Due to the complete absence of voluntary muscle contraction, SCI is followed by a rapid loss of skeletal muscle mass. SCI has several physical, psychological, social, and economic consequences.Case presentationA 68-years-old woman presented with weakness and numbness of all four limbs. She also had sarcopenia, malnutrition, and dependency on activities of daily living (ADL). In addition, the patient was at a risk of SCI complications. Magnetic resonance imaging (MRI) showed destruction of the vertebral bodies at the level of the 5th and 6th cervical area, tuberculous abscesses of the paravertebral and longus colli muscles. The patient underwent debridement and spinal stabilization. However, the patient was at a risk of developing SCI complications. In these patients, the Physical Rehabilitation and Medicine (PRM) strategy focuses on improving medical conditions, including preventing secondary complications, promoting neurological recovery, and optimizing function.ConclusionThis case highlights the importance of PRM intervention in assessing functional disorders in the elderly to improve their quality of life (QOL).
Drivers of vulnerability to health and wellbeing challenges in informal settlements
Authors: Chumo Ivy , Kabaria Caroline , Shankland Alex , Mberu Blessing 
Published: February 2023

IntroductionIt is widely acknowledged that vulnerable populations are hit very hard, both in the short and long term, when their health and wellbeing needs are not met. Despite the efforts at different levels to protect and promote their health and wellbeing, older persons, people with disabilities and children heads of households, continue to face significant social, economic and cultural difficulties in relation to health and wellbeing inequities. While rights to health and wellbeing are constitutionally guaranteed, and strategies can be advanced to reduce vulnerable situations, challenges persists and yet societies, communities, and individual factors that engender vulnerability are understudied and remain poorly understood. Situating our findings and understandings within CLUVA social vulnerability framework, allows us to adapt a conceptual framework for understanding vulnerability to health and wellbeing challenges across different groups in informal urban space. We used CLUVA social vulnerability framework to explore and uncover drivers of vulnerability to health and wellbeing challenges among the vulnerable and marginalized groups using the governance diaries approach.MethodsThis was an ethnographic study, using governance diaries with 24 participants in Korogocho and Viwandani informal settlements in Nairobi, Kenya. The governance diaries approach involved bi-weekly governance in-depth interviews (IDIs) with study participants for 4 months, complemented with observations, reflections, participant diaries and informal discussions. We used framework analysis methodology.ResultsWe identified several interlinked drivers and grouped them as individual, community, societal and structural level factors.DiscussionA comprehensive view of drivers at different levels will help actors engage in more expansive and collaborative thinking about strategies that can effectively reduce health and wellbeing challenges.ConclusionThe factors identified come together to shape functioning and capabilities of vulnerable groups in informal settlements. Beyond applying a more comprehensive concept of understanding health and wellbeing challenges, It is important to understand the drivers of vulnerability to health and wellbeing challenges from the perspective of marginalized and vulnerable populations. Particularly for local urban planning, the information should blend routine data with participatory assessment within different areas and groups in the city.
Recommendations and optimal approaches to robotic-assisted partial nephrectomy: A consensus of Brazilian experts
Authors: Faria Eliney Ferreira , Moschovas Marcio Covas , Vaz Carlos , Pompeo Alexandre , Santos Alexandre , Stievano Alexandre , Berger Andre , Carneiro Arie , Dourado Aurus , Colombo Jose Roberto , Passerotti Carlo , Andreoni Cassio , Fraga Clovis , Guglielmetti Giuliano , Lemos Gustavo , Guimarães Gustavo , Nogueira Lucas , Rocha Marcos , Melo Pablo , Arantes Paulo , Romanelli Pedro , Tourinho Rafael , Nishimoto Ricardo , Machado Roberto , Reis Rodolfo , Frota Rodrigo , Guida Romulo , Dubeux Victor , Gualberto Rodrigo , Tobias-Machado Marcos 
Published: February 2023

ObjectiveRobotic-assisted partial nephrectomy (RAPN) is established as the gold standard approach to treating small renal masses. However, numerous technical challenges and concepts related to this approach are still under discussion and are not consensus among surgeons from different centers. We performed an online questionnaire with multiple topics about RAPN and selected high-volume surgeons from referral centers in Brazil to achieve a consensus.MethodsWe implemented an online consensus of 29 experts selected based on surgical expertise and competence in analyzing the published literature. Based on the collected literature and current Guidelines (NCCN, AUA, and EAU) we created a questionnaire with 131 questions and administered it to all participants. The statements and the Delphi technique design were combined in a single round of questions. The answers were reviewed, 70% of concordance was considered a consensus, and a final manuscript with recommendations was developed.ResultsWe divided our results into 25 subtopics that included all questions and discussions of the questionnaire, including preoperative settings, surgical technique, pathological analysis, technology use, and challenging cases. Some areas had limited data in the literature, and these potential limitations were addressed and discussed on each topic.ConclusionRAPN is the standard surgical treatment for renal masses in the centers of robotic surgery. Among the important topics of this study, we recommend always performing the first RAPN cases with proctors’ assistance, conducting preoperative planning using good-quality imaging exams, minimizing the amount of renal parenchyma removed, and achieving appropriate hemostatic suture while reducing renal parenchyma ischemia.
The efficacy of peripheral nerve block on postoperative catheter-related bladder discomfort in males: A systematic review and meta-analysis
Authors: Bao Xingjun , Liu Ming , Li Jie , Yao Huibao , Liu Hongquan , Tang Gonglin , Wang Xiaofeng , Zhou Zhongbao , Wu Jitao , Cui Yuanshan 
Published: February 2023

ObjectiveTo determine the efficacy of peripheral nerve block (PNB) in preventing postoperative catheter-related bladder discomfort (CRBD).MethodsUp to July 1, 2022, the PubMed, Embase and Cochrane Central Register of Controlled Trials databases were searched, and all articles that met the PICOS (Patient, Intervention, Comparator, Outcome, Study design) criteria were enrolled. The included trials were evaluated using the Cochrane Collaboration's tool. Patients in the block group received bilateral PNB, while those in the non-block group did not need any additional procedure or simply achieved “sham block”. CRBD was quantified using the visual analog scale (VAS) score, which was questioned and recorded at 0–1 h, 1–2 h, 4–8 h, 8–12 h and 12–24 h intervals. The incidences of CRBD, moderate to severe CRBD and postoperative nausea and vomiting (PONV) were meta-analysed.ResultsSix trials with a total of 544 patients were considered. First, the block group had a lower incidence of CRBD than the non-block group at 0–1 h (OR 0.22; 95% CI, 0.18–0.08; P &lt; 0.0001), 1–2 h (OR 0.14; 95% CI, 0.08–0.26; P &lt; 0.00001), 4–8 h (OR 0.27; 95% CI, 0.13 to 0.58; P &lt; 0.0008) and 8–12 h (OR 0.51; 95% CI, 0.30 to 0.87; P = 0.01). Second, the block group showed a lower incidence of moderate to severe CRBD than the non-block group at 0–1 h, 1–2 h and 4–8 h, and the ORs were 0.12 (95% CI, 0.03 to 0.49; P = 0.003), 0.17 (95% CI, 0.08 to 0.37; P &lt; 0.00001) and 0.29 (95% CI, 0.15 to 0.55; P = 0.0002),respectively. Finally, the block group was significantly associated with a decreased incidence of PONV (OR, 0.14; 95% CI, 0.05 to 0.39; P = 0.0002).ConclusionThis meta-analysis suggested that PNB markedly reduced the incidence and severity of early postoperative CRBD and decreased the occurrence of PONV.
Effect of Health Intervention via Web-Based Education on Improving Information-Motivation-Behavioral Skills Related to HPV Vaccination Among Chinese Female College Students
Authors: Wang Hong , Wang Xiaoyang , Chen Peipei , Xu Huifang , Liu Yin , Kang Ruihua , Zheng Liyang , Sun Xingyuan , Sun Xibin , Zhang Shaokai 
Published: February 2023

Objectives: The human papillomavirus (HPV) vaccination rate is generally low in China. This study aimed to assess the effect of web-based education on improving information-motivation-behavior skills related to HPV vaccination among Chinese female college students.Methods: From February to May 2020, female students were recruited from a university and divided into intervention and control groups. The intervention group received 7 days of HPV-related web-based education. Related information were collected using questionnaires in the baseline, 7 days, 1 month, and 3 months after the intervention. Chi-square test and repeated ANOVA were used to compare the differences between the two groups in the four surveys.Results: A total of 449 students (235 in the intervention and 214 in the control group) were included in the analysis. There were no statistical differences in demographic information between the two groups. Compared with the control group, students in the intervention group showed a richer knowledge and subjective norms of HPV vaccination (p &lt; 0.05).Conclusion: The study provides preliminary support for a health intervention via web-based education in increasing HPV vaccination information among female college students.