bluuebunny/crossref_metadata_2025_split huggingface dataset:
Reversible bipolar thermopower of ionic thermoelectric polymer composite for cyclic energy generation
Authors: Chi Cheng , Liu Gongze , An Meng , Zhang Yufeng , Song Dongxing , Qi Xin , Zhao Chunyu , Wang Zequn , Du Yanzheng , Lin Zizhen , Lu Yang , Huang He , Li Yang , Lin Chongjia , Ma Weigang , Huang Baoling , Du Xiaoze , Zhang Xing 
Published: January 2023

AbstractThe giant thermopower of ionic thermoelectric materials has attracted great attention for waste-heat recovery technologies. However, generating cyclic power by ionic thermoelectric modules remains challenging, since the ions cannot travel across the electrode interface. Here, we reported a reversible bipolar thermopower (+20.2 mV K−1 to −10.2 mV K−1) of the same composite by manipulating the interactions of ions and electrodes. Meanwhile, a promising ionic thermoelectric generator was proposed to achieve cyclic power generation under a constant heat course only by switching the external electrodes that can effectively realize the alternating dominated thermodiffusion of cations and anions. It eliminates the necessity to change the thermal contact between material and heat, nor does it require re-establish the temperature differences, which can favor improving the efficiency of the ionic thermoelectrics. Furthermore, the developed micro-thermal sensors demonstrated high sensitivity and responsivity in light detecting, presenting innovative impacts on exploring next-generation ionic thermoelectric devices.
Post-transcriptional control of a stemness signature by RNA-binding protein MEX3A regulates murine adult neurogenesis
Authors: Domingo-Muelas Ana , Duart-Abadia Pere , Morante-Redolat Jose Manuel , Jordán-Pla Antonio , Belenguer Germán , Fabra-Beser Jaime , Paniagua-Herranz Lucía , Pérez-Villalba Ana , Álvarez-Varela Adrián , Barriga Francisco M. , Gil-Sanz Cristina , Ortega Felipe , Batlle Eduard , Fariñas Isabel 
Published: January 2023

AbstractNeural stem cells (NSCs) in the adult murine subependymal zone balance their self-renewal capacity and glial identity with the potential to generate neurons during the lifetime. Adult NSCs exhibit lineage priming via pro-neurogenic fate determinants. However, the protein levels of the neural fate determinants are not sufficient to drive direct differentiation of adult NSCs, which raises the question of how cells along the neurogenic lineage avoid different conflicting fate choices, such as self-renewal and differentiation. Here, we identify RNA-binding protein MEX3A as a post-transcriptional regulator of a set of stemness associated transcripts at critical transitions in the subependymal neurogenic lineage. MEX3A regulates a quiescence-related RNA signature in activated NSCs that is needed for their return to quiescence, playing a role in the long-term maintenance of the NSC pool. Furthermore, it is required for the repression of the same program at the onset of neuronal differentiation. Our data indicate that MEX3A is a pivotal regulator of adult murine neurogenesis acting as a translational remodeller.
The thickness dependence of quantum oscillations in ferromagnetic Weyl metal SrRuO3
Authors: Kar Uddipta , Singh Akhilesh Kr. , Hsu Yu-Te , Lin Chih-Yu , Das Bipul , Cheng Cheng-Tung , Berben M. , Yang Song , Lin Chun-Yen , Hsu Chia-Hung , Wiedmann S. , Lee Wei-Cheng , Lee Wei-Li 
Published: January 2023

AbstractIn a thin Weyl semimetal, a thickness dependent Weyl-orbit quantum oscillation was proposed to exist, originating from a nonlocal cyclotron orbit via electron tunnelings between top and bottom Fermi-arc surface states. Here, magneto-transport measurements were carried out on untwinned Weyl metal SrRuO3 thin films. In particular, quantum oscillations with a frequency Fs1 ≈ 30 T were identified, corresponding to a small Fermi pocket with a light effective mass. Its oscillation amplitude appears to be at maximum for thicknesses in a range of 10 to 20 nm, and the phase of oscillation exhibits a systematic change with film thickness. The constructed Landau fan diagram shows an unusual concave downward curvature in the 1/μ0Hn-n curve, where n is the Landau level index. From thickness and field-orientation dependence, the Fs1 oscillation is attributed to be of surface origin. Those findings can be understood within the framework of the Weyl-orbit quantum oscillation effect with non-adiabatic corrections.
Expansion of ventral foregut is linked to changes in the enhancer landscape for organ-specific differentiation
Authors: Wong Yan Fung , Kumar Yatendra , Proks Martin , Herrera Jose Alejandro Romero , Rothová Michaela Mrugala , Monteiro Rita S. , Pozzi Sara , Jennings Rachel E. , Hanley Neil A. , Bickmore Wendy A. , Brickman Joshua M. 
Published: January 2023

AbstractCell proliferation is fundamental for almost all stages of development and differentiation that require an increase in cell number. Although cell cycle phase has been associated with differentiation, the actual process of proliferation has not been considered as having a specific role. Here we exploit human embryonic stem cell-derived endodermal progenitors that we find are an in vitro model for the ventral foregut. These cells exhibit expansion-dependent increases in differentiation efficiency to pancreatic progenitors that are linked to organ-specific enhancer priming at the level of chromatin accessibility and the decommissioning of lineage-inappropriate enhancers. Our findings suggest that cell proliferation in embryonic development is about more than tissue expansion; it is required to ensure equilibration of gene regulatory networks allowing cells to become primed for future differentiation. Expansion of lineage-specific intermediates may therefore be an important step in achieving high-fidelity in vitro differentiation.
Pyramidal cell types drive functionally distinct cortical activity patterns during decision-making
Authors: Musall Simon , Sun Xiaonan R. , Mohan Hemanth , An Xu , Gluf Steven , Li Shu-Jing , Drewes Rhonda , Cravo Emma , Lenzi Irene , Yin Chaoqun , Kampa Björn M. , Churchland Anne K. 
Published: January 2023

AbstractUnderstanding how cortical circuits generate complex behavior requires investigating the cell types that comprise them. Functional differences across pyramidal neuron (PyN) types have been observed within cortical areas, but it is not known whether these local differences extend throughout the cortex, nor whether additional differences emerge when larger-scale dynamics are considered. We used genetic and retrograde labeling to target pyramidal tract, intratelencephalic and corticostriatal projection neurons and measured their cortex-wide activity. Each PyN type drove unique neural dynamics, both at the local and cortex-wide scales. Cortical activity and optogenetic inactivation during an auditory decision task revealed distinct functional roles. All PyNs in parietal cortex were recruited during perception of the auditory stimulus, but, surprisingly, pyramidal tract neurons had the largest causal role. In frontal cortex, all PyNs were required for accurate choices but showed distinct choice tuning. Our results reveal that rich, cell-type-specific cortical dynamics shape perceptual decisions.
Mobility recorded by wearable devices and gold standards: the Mobilise-D procedure for data standardization
Authors: Palmerini Luca , Reggi Luca , Bonci Tecla , Del Din Silvia , Micó-Amigo M. Encarna , Salis Francesca , Bertuletti Stefano , Caruso Marco , Cereatti Andrea , Gazit Eran , Paraschiv-Ionescu Anisoara , Soltani Abolfazl , Kluge Felix , Küderle Arne , Ullrich Martin , Kirk Cameron , Hiden Hugo , D’Ascanio Ilaria , Hansen Clint , Rochester Lynn , Mazzà Claudia , Chiari Lorenzo 
Published: January 2023

AbstractWearable devices are used in movement analysis and physical activity research to extract clinically relevant information about an individual’s mobility. Still, heterogeneity in protocols, sensor characteristics, data formats, and gold standards represent a barrier for data sharing, reproducibility, and external validation. In this study, we aim at providing an example of how movement data (from the real-world and the laboratory) recorded from different wearables and gold standard technologies can be organized, integrated, and stored. We leveraged on our experience from a large multi-centric study (Mobilise-D) to provide guidelines that can prove useful to access, understand, and re-use the data that will be made available from the study. These guidelines highlight the encountered challenges and the adopted solutions with the final aim of supporting standardization and integration of data in other studies and, in turn, to increase and facilitate comparison of data recorded in the scientific community. We also provide samples of standardized data, so that both the structure of the data and the procedure can be easily understood and reproduced.
Investigating the effects of cyclic topology on the performance of a plastic degrading enzyme for polyethylene terephthalate degradation
Authors: Hayes Heather C. , Luk Louis Y. P. 
Published: January 2023

AbstractAgitation is a commonly encountered stress for enzymes during all stages of production and application, but investigations that aim to improve their tolerance using topological engineering have yet to be reported. Here, the plastic-degrading enzyme IsPETase was cyclized in a range of topologies including a cyclic monomer, cyclic dimer and catenane using SpyTag/SpyCatcher technologies, and their tolerance towards different stresses including mechanical agitation was investigated. The cyclic dimer and catenane topologies were less susceptible to agitation-induced inactivation resulting in enhancement of polyethylene terephthalate (PET) degradation. While contrary to conventional belief, cyclic topologies did not improve tolerance of IsPETase towards heat or proteolytic treatment, the close proximity of active sites in the dimeric and catenane variants was found to enhance PET conversion into small soluble products. Together, these findings illustrate that it is worthwhile to explore the topology engineering of enzymes used in heterogeneous catalysis as it improves factors that are often overlooked in homogeneous catalysis studies.
Macrophage numbers in the marginal area of sarcomas predict clinical prognosis
Authors: Umakoshi Michinobu , Nakamura Akiko , Tsuchie Hiroyuki , Li Zhuo , Kudo-Asabe Yukitsugu , Miyabe Ken , Ito Yukinobu , Yoshida Makoto , Nagasawa Hiroyuki , Okada Kyoji , Nanjo Hiroshi , Maeda Daichi , Miyakoshi Naohisa , Tanaka Masamitsu , Goto Akiteru 
Published: January 2023

AbstractEven when treated comprehensively by surgery, chemotherapy, and radiotherapy, soft-tissue sarcoma has an unfavorable outcome. Because soft-tissue sarcoma is rare, it is the subject of fewer clinicopathological studies, which are important for clarifying pathophysiology. Here, we examined tumor-associated macrophages in the intratumoral and marginal areas of sarcomas to increase our knowledge about the pathophysiology. Seventy-five sarcoma specimens (not limited to a single histological type), resected at our institution, were collected, and the number of CD68-, CD163-, and CD204-positive macrophages in the intratumoral and marginal areas was counted. We then performed statistical analysis to examine links between macrophage numbers, clinical factors, and outcomes. A high number of macrophages positive for all markers in both areas was associated with worse disease-free survival (DFS). Next, we divided cases according to the FNCLCC classification (Grade 1 and Grades 2/3). In the Grade 1 group, there was no significant association between macrophage number and DFS. However, in the Grade 2/3 group, high numbers of CD163- and CD204-positive macrophages in the marginal area were associated with poor DFS. By contrast, there was no significant difference between the groups with respect to high or low numbers of CD68-, CD163-, or CD204-positive macrophages in the intratumoral area. Multivariate analysis identified the number of CD163- and CD204-positive macrophages in the marginal area as an independent prognostic factor. Macrophage numbers in the marginal area of soft-tissue sarcoma may better reflect clinical behavior.
Catechol-O-methyl transferase suppresses cell invasion and interplays with MET signaling in estrogen dependent breast cancer
Authors: Janacova Lucia , Stenckova Michaela , Lapcik Petr , Hrachovinova Sarka , Bouchalova Pavla , Potesil David , Hrstka Roman , Müller Petr , Bouchal Pavel 
Published: January 2023

AbstractCatechol-O-methyl transferase (COMT) is involved in detoxification of catechol estrogens, playing cancer-protective role in cells producing or utilizing estrogen. Moreover, COMT suppressed migration potential of breast cancer (BC) cells. To delineate COMT role in metastasis of estrogen receptor (ER) dependent BC, we investigated the effect of COMT overexpression on invasion, transcriptome, proteome and interactome of MCF7 cells, a luminal A BC model, stably transduced with lentiviral vector carrying COMT gene (MCF7-COMT). 2D and 3D assays revealed that COMT overexpression associates with decreased cell invasion (p < 0.0001 for Transwell assay, p < 0.05 for spheroid formation). RNA-Seq and LC-DIA-MS/MS proteomics identified genes associated with invasion (FTO, PIR, TACSTD2, ANXA3, KRT80, S100P, PREX1, CLEC3A, LCP1) being downregulated in MCF7-COMT cells, while genes associated with less aggressive phenotype (RBPMS, ROBO2, SELENBP, EPB41L2) were upregulated both at transcript (|log2FC|> 1, adj. p < 0.05) and protein (|log2FC|> 0.58, q < 0.05) levels. Importantly, proteins driving MET signaling were less abundant in COMT overexpressing cells, and pull-down confirmed interaction between COMT and Kunitz-type protease inhibitor 2 (SPINT2), a negative regulator of MET (log2FC = 5.10, q = 1.04−7). In conclusion, COMT may act as tumor suppressor in ER dependent BC not only by detoxification of catechol estrogens but also by suppressing cell invasion and interplay with MET pathway.
Palladium supported magnetic Fucus Vesiculosus extract as a natural and novel catalyst for the synthesis of N-alkyl-2-(4-methyl-1-oxoisoquinolin-2(1H)-yl)-2-phenylacetamide derivatives
Authors: Yousefnejad Faeze , Bahadorikhalili Saeed , Esmkhani Maryam , Adib Mehdi , Javanshir Shahrzad , Hosseini Samanehsadat , Larijani Bagher , Mahdavi Mohammad 
Published: January 2023

AbstractIn this paper, a novel catalyst is introduced based on the immobilization of palladium onto magnetic Fucus Vesiculosus extract (Pd@mFuVe catalyst). For the synthesis of Pd@mFuVe catalyst, Fucus Vesiculosus extract is obtained from the plant source, followed by the synthesis of superparamagnetic iron oxide nanoparticles (SPION) onto the extract. The catalyst is characterized by several methods, including scanning electron microscopy (SEM), energy-dispersive X-ray spectroscopy (EDS), FT-IR spectroscopy, vibrating sample magnetometer (VSM), powder X-ray diffraction analysis (XRD), and inductively coupled plasma (ICP). The activity of Pd@mFuVe catalyst is studied in the synthesis of N-alkyl-2-(4-methyl-1-oxoisoquinolin-2(1H)-yl)-2-phenylacetamides. The products were synthesized in three steps, the synthesis of 2-iodobenzoic acid from 2-aminobenzoic acid, which participated in a multicomponent reaction with allylamine, aldehydes, and isocyanides, followed by a cyclization reaction, catalyzed by Pd@mFuVe catalyst. The product yields are high and the catalyst showed good reusability after 5 sequential runs. The most significant, Pd@mFuVe catalyst is fabricated from a plant extract source as a green support for the catalyst.
Breakdown of intention-based outcome evaluation after transient right temporoparietal junction deactivation
Authors: Zhang Junfeng , Sun Sai , Zhou Chengyan , Cai Yaochun , Liu Hao , Yang Zhaoyang , Yu Rongjun 
Published: January 2023

AbstractPeople judge the nature of human behaviors based on underlying intentions and possible outcomes. Recent studies have demonstrated a causal role of the right temporoparietal junction (rTPJ) in modulating both intention and intention-based outcome evaluations during social judgments. However, these studies mainly used hypothetical scenarios with socially undesirable contexts (bad/neutral intentions and bad/neutral outcomes), leaving the role of rTPJ in judging good intentions and good outcomes unclear. In the current study, participants were instructed to make goodness judgments as a third party toward the monetary allocations from one proposer to another responder. Critically, in some cases, the initial allocation by the proposer could be reversed by the computer, yielding combinations of good/bad intentions (of the proposer) with good/bad outcomes (for the responder). Anodal (n = 20), cathodal (n = 21), and sham (n = 21) transcranial direct current stimulation (tDCS) over the rTPJ were randomly assigned to 62 subjects to further examine the effects of stimulation over the rTPJ in modulating intention-based outcome evaluation. Compared to the anodal and sham stimulations, cathodal tDCS over the rTPJ reduced the goodness ratings of good/bad outcomes when the intentions were good, whereas it showed no significant effect on outcome ratings under unknown and bad intentions. Our results provide the first evidence that deactivating the rTPJ modulates outcome evaluation in an intention-dependent fashion, mainly by reducing the goodness rating towards both good/bad outcomes when the intentions are good. Our findings argue for a causal role of the rTPJ in modulating intention-based social judgments and point to nuanced effects of rTPJ modulation.
Optimizing in vitro nutrient and ex vitro soil mediums-driven responses for multiplication, rooting, and acclimatization of pineapple
Authors: Lakho Maqsood Ahmed , Jatoi Mushtaque Ahmed , Solangi Najamuddin , Abul-Soad Adel Ahmed , Qazi Muneer Ahmed , Abdi Gholamreza 
Published: January 2023

AbstractA comprehensive study was carried out on in vitro multiplication and rooting using the medium enriched with different plant growth regulators and acclimatization of pineapple cv. ‘Smooth Cayenne’ using different soil growing substrates. The significantly highest shoot buds (Avg. 16.7) were obtained on the medium comprising 2.0 mg L−1 BA (6-Benzylaminopurine). Results showed that 1.0 mg L−1 IBA (Indole-3-butyric acid) increased the thickness and length of white adventitious roots and resulted in a significantly highest number of roots (Avg. 8) and root length (6.15 cm). Plantlets with healthy, multiple roots were transplanted in several soil combinations of river silt, bolhari (yellow sand), and peat moss. However, the significantly highest survival (100%) of plantlets in the greenhouse was obtained on the soil medium containing only peat moss. Furthermore, soil mixtures of bolhari and peat moss (1:1) and river silt alone exhibited 98.9% and 95.1% survivability of plantlets, which was also considered equally significant (at 5% probability level). The in vitro and ex vitro protocols optimized in the current study can be applied commercially for pineapple crop worldwide.
Prediction of line heating deformation on sheet metal based on an ISSA–ELM model
Authors: Li Lei , Qi Shukang , Zhou Honggen , Wang Lei 
Published: January 2023

AbstractA prediction method based on an improved salp swarm algorithm (ISSA) and extreme learning machine (ELM) was proposed to improve line heating and forming. First, a three-dimensional transient numerical simulation of line heating and forming was carried out by applying a finite element simulation, and the influence of machining parameters on deformation was studied. Second, a prediction model for the ELM network was established based on simulation data, and the deformation of hull plate was predicted by the training network. Additionally, swarm intelligence optimization, particle swarm optimization (PSO), the seagull optimization algorithm (SOA), and the salp swarm algorithm (SSA) were studied while considering the shortcomings of the ELM, and the ISSA was proposed. Input weights and hidden layer biases of the ELM model were optimized to increase the stability of prediction results from the PSO, SOA, SSA and ISSA approaches. Finally, it was shown that the prediction effect of the ISSA–ELM model was superior by comparing and analyzing the prediction effect of each prediction model for line heating and forming.
Single-cell profiling reveals differences between human classical adenocarcinoma and mucinous adenocarcinoma
Authors: Hu Fang-Jie , Li Ying-Jie , Zhang Li , Ji Deng-Bo , Liu Xin-Zhi , Chen Yong-Jiu , Wang Lin , Wu Ai-Wen 
Published: January 2023

AbstractColorectal cancer is a highly heterogeneous disease. Most colorectal cancers are classical adenocarcinoma, and mucinous adenocarcinoma is a unique histological subtype that is known to respond poorly to chemoradiotherapy. The difference in prognosis between mucinous adenocarcinoma and classical adenocarcinoma is controversial. Here, to gain insight into the differences between classical adenocarcinoma and mucinous adenocarcinoma, we analyse 7 surgical tumour samples from 4 classical adenocarcinoma and 3 mucinous adenocarcinoma patients by single-cell RNA sequencing. Our results indicate that mucinous adenocarcinoma cancer cells have goblet cell-like properties, and express high levels of goblet cell markers (REG4, SPINK4, FCGBP and MUC2) compared to classical adenocarcinoma cancer cells. TFF3 is essential for the transcriptional regulation of these molecules, and may cooperate with RPS4X to eventually lead to the mucinous adenocarcinoma mucus phenotype. The observed molecular characteristics may be critical in the specific biological behavior of mucinous adenocarcinoma.
A generic hierarchical model of organic matter degradation and preservation in aquatic systems
Authors: Shang Haitao 
Published: January 2023

AbstractOrganic matter degradation and preservation are crucial components of Earth’s carbon cycle. Empirical and phenomenological models usually contain parameters determined by site-specific data and focus on different aspects of the decay and accretion characteristics. To investigate more fundamental mechanisms, this study suggests a hierarchical model that links microscopic physical quantities to macroscopic degradation and preservation patterns. This mechanistic model predicts several commonly observed phenomena, including the lognormal distribution of degradation rate constants, the recalcitrance-dependent sensitivity to temperature, the dependence of a heterogeneous organic-matter system’s persistence on its complexity, logarithmic-time decay, and power-law degradation behavior. The theoretical predictions of this model are consistent with the observational data from marine and lake environments. This hierarchical model may provide a step towards a fundamental theory of organic matter degradation and preservation in aquatic and other ecosystems.