bluuebunny/crossref_metadata_2025_split huggingface dataset:
Ginger essential oil prevents NASH progression by blocking the NLRP3 inflammasome and remodeling the gut microbiota-LPS-TLR4 pathway
Authors: Sheen Lee-Yan , Panyod Suraphan , Wu Wei-Kai , Hsieh Ya-Chi , Tseng Yea-Jing , Peng Sin-Yi , Chen Rou-An , Huang Huai-Syuan , Chen Yi-Hsun , Shen Ting-Chin David , Ho Chi-Tang , Liu Chun-Jen , Chuang Hsiao-Li , Huang Chi-Chang , Wu Ming-Shiang 
Published: August 2023

Abstract

BACKGROUND
Diet and gut microbiota contribute to non-alcoholic steatohepatitis (NASH) progression. High-fat diets (HFDs) change gut microbiota compositions, induce gut dysbiosis, and intestinal barrier leakage, which facilitates portal influx of pathogen-associated molecular patterns including lipopolysaccharides (LPS) to the liver and triggers inflammation in NASH. Current therapeutic drugs for NASH have adverse side effects; however, several foods and herbs that exhibit hepatoprotection could be an alternative method to prevent NASH.
METHODS
We investigated ginger essential oil (GEO) against palm oil-containing HFDs in LPS-injected murine NASH model.
RESULTS
GEO reduced plasma alanine aminotransferase levels and hepatic pro-inflammatory cytokine levels; and increased antioxidant catalase, glutathione reductase, and glutathione levels to prevent NASH. GEO alleviated hepatic inflammation through mediated NLR family pyrin domain-containing 3 (NLRP3) inflammasome and LPS/Toll-like receptor four (TLR4) signaling pathways. GEO further increased beneficial bacterial abundance and reduced NASH-associated bacterial abundance.
CONCLUSION
Hence, GEO prevents NASH by inhibiting hepatic inflammation and modulating the gut microbiota and its metabolite-related pathway. Additionally, GEO offers a promising application as a dietary supplement for the prevention of NASH.
Study of the effect of cAMP signaling pathway on HTR8/SV-neo cell line proliferation,invasion and migration
Authors: Sun Chao , Mei Jiaoqi , Yi Hongyan , Song Mengyi , Ma Yanlin , Huang Yuanhua 
Published: August 2023

Abstract
Pre-eclampsia (PE) is thought to be related to placental dysfunction, particularly poor extravillous trophoblast (EVT) invasion and migration abilities. However, the pathogenic mechanism is not yet fully understood. Here, we used the HTR8/SV-neo cell line as a model for the in vitro functional study of human extravillous trophoblasts. HTR8/SV-neo cells were treated with different concentrations of Forskolin solution (cAMP pathway-specific agonist) to alter intracellular cAMP level. Taking the DMSO group as the control group, firstly, cAMP assay kit was used to detect the cAMP concentration in HTR8/SV-neo cells treated with different concentration of Forskolin, and the cell proliferation function was detected by cell growth curve drawing and the colony formation experiment. The invasiveness and migration of cells were observed by Transwell experiments, and the expression of intracellular EMT-related markers was further evaluated by qPCR and WB experiments. According to our research, the intracellular cAMP level in HTR8/SV-neo cells was increased in a dose-dependently manner, and the proliferation, invasion and migration abilities of HTR8/SV-neo cells were significantly enhanced. Further detection by qPCR and WB experiments proved that the expression of EMT and angiogenesis markers in cells were up-regulated, including MMP2, PECAM, N-cadherin and VE-cadherin. In addition, with the increase of intracellular cAMP level, the phosphorylation level of intracellular MAPK signaling pathway was significantly increased. Thus, these results provide a novel rationale for the treatment of PE patients by improving the poor placental environment.
COPZ1 regulates ferroptosis through NCOA4-mediated ferritinophagy in lung adenocarcinoma
Authors: Wu Anbang , Yang Hongmin , Xiao Tengfei , Gu Wangning , Li He , Chen Pan 
Published: August 2023

Abstract
Background
Ferroptosis, a type of autophagy-dependent cell death, is intricately related to the pathogenesis of lung adenocarcinoma (LUAD). The research focus of this study was to investigate the involvement of coatomer protein complex I subunit zeta 1 (COPZ1) in ferroptosis and ferritinophagy in LUAD.
Methods
In this study, clinical samples of LUAD and para-carcinoma tissues were gathered. Lentiviral vectors were utilized to establish COPZ1-deficient LUAD cell model and xenograft model. These models were analyzed to assess tumor growth, lipid peroxidation levels, autophagy activation, and iron metabolism. To demonstrate the regulatory effects of COPZ1 on its downstream targets, qRT-PCR, Western blot, and co-immunoprecipitation (co-IP) assay were conducted. Moreover, NCOA4 knockdown plasmid was applied. The location of ferritin and lysosomes were examined using immunofluorescence staining.
Results
The study revealed that LUAD tissues and cells exhibited elevated expression of COPZ1. COPZ1 silencing inhibited xenograft tumor growth and induced apoptosis. COPZ1 silencing also promoted the accumulation of ROS, Fe2+, MDA and 4-HNE while reducing GSH-Px levels. In addition, COPZ1 was found to directly bind to nuclear receptor coactivator 4 (NCOA4). COPZ1 silencing inhibit the expression of FTH1 and promoted the expression of NCOA4 and LC3. NCOA4 knockdown reversed the iron metabolism and lipid peroxidation regulation induced by COPZ1 silencing. Furthermore, COPZ1 silencing induced the translocation of ferritin to lysosomes for degradation, while NCOA4 knockdown disrupted this process.
Conclusion
This study presents novel findings showing that COPZ1 regulates NCOA4-mediated ferritinophagy and ferroptosis. These results offer new insights into the development and potential treatment of LUAD.
Analysis of Different Transistor Sram Structures Using Different Technologies
Authors: R SUGUNA , S MONISHA 
Published: August 2023

Abstract
In recent years, the need for power efficient memory design has increased drastically. SRAM design is commonly used in network-on-chip and system-on-chip VLSI design for low power and high speed operation. For high speed processor design SRAM is mainly used in cache memories as it provides a direct interface with processing unit which is not possible using DRAM cells. In order to achieve area-delay-power trade off depending on the applications, various SRAM designs in transistor level is analyzed earlier. Some power gating SRAM design focuses on reducing the power consumption by adding sleep transistors. For some high-performance design SRAM works by performing the read and write operation separately with individual control signals. This proposed work focuses on analyzing various transistor SRAM structures used in various applications. This paper also evaluates various parameters such as power consumption and transistor count. All the design is formed using DSCH2 and simulations have been carried out on in different technologies using MICROWIND tool.
Sex divergences in brain transcriptomics of juvenile Cynomolgus macaques
Authors: Kabbej Nadia , Ashby Frederick J. , Riva Alberto , Gamlin Paul , Mandel Ron , Kunta Aishwarya , Rouse Courtney J. , Heldermon Coy 
Published: August 2023

Abstract
Background: Behavioral, social, and physical characteristics are posited to distinguish the sexes, yet research on transcription-level sexual differences in the brain is limited. Here, we investigated sexually divergent brain transcriptomics in prepubertal cynomolgus macaques, a commonly used surrogate species to humans.
Methods: A transcriptomic profile using RNA sequencing was generated for the temporal lobe, ventral midbrain, and cerebellum of 3 female and 3 male cynomolgus macaques previously treated with an Adeno-associated virus vector mix. Statistical analyses to determine differentially expressed protein-coding genes in all three lobes were conducted using DeSeq2 with a false discovery rate corrected P value of .05.
Results: We identified target genes in the temporal lobe, ventral midbrain, and cerebellum with functions in translation, immunity, behavior, and neurological disorders that exhibited statistically significant sexually divergent expression.
Conclusions: We provide potential mechanistic insights to the epidemiological differences observed between the sexes with regards to mental health and infectious diseases, such as COVID19. Our results provide pre-pubertal information on sexual differences in non-human primate brain transcriptomics and may provide insight to health disparities between the biological sexes in humans.
Preoperative Systemic immune-inflammation index as a prognostic indicator for patients with urothelial carcinoma
Authors: Zheng Jianxiong , Peng Lei , Zhang Shaohua , Liao Haiyang , Hao jiayao , Wu Song , Shen Haili 
Published: August 2023

Abstract
Background
The systemic immune-inflammation index (SII) is a cost-efficient indicator for carcinoma prognosis. However, its utility in urothelial carcinoma (UC) prognosis is disputed. This meta-analysis aims to assess SII's prognostic value in UC.
Methods
A thorough search of databases including PubMed, Web of Science, Embase, Cochrane Library, and Scopus, was conducted to find studies until January 11, 2023. Eligibility criteria were applied to select studies. Hazard ratios (HRs) and 95% confidence intervals (CIs) were extracted from selected studies and compiled in a meta-analysis to gauge SII's association with survival outcomes such as overall survival (OS), cancer-specific survival (CSS), recurrence-free survival (RFS), and progression-free survival (PFS).
Results
This analysis includes 19 studies with 11,348 UC patients. It was found that high SII significantly correlated with worse OS in UC patients (HR 1.430, 95% CI 1.237–1.653, P < 0.001). High SII values also linked with poorer CSS (HR 1.913, 95% CI 1.473–2.485, P < 0.001), RFS (HR 1.240, 95% CI 1.097–1.403, P < 0.001), and PFS (HR 1.844, 95% CI 1.488–2.284, P < 0.001) compared to low SII values. Subgroup analysis revealed SII's consistent prognostic value in UC across races, carcinoma types, sample sizes, and SII cut-off values, suggesting its potential as a prognostic indicator in UC patients.
Conclusions
Current evidence suggests SII as a promising, cost-efficient predictor in UC patients. This meta-analysis indicates SII's potential as a valuable prognostication tool in UC patients.
Tannic acid as building block constructing injectable hydrogel and regulating microglial phenotype to enhance neuroplasticity for post-stroke rehabilitation
Authors: Liu Zongjian , Zhang Shulei , Ran Yuanyuan , Geng Huimin , Gao Fuhai , Tian Guiqin , Feng Zengguo , Xi Jianing , YE LIN , Su Wei 
Published: August 2023

Abstract

Background Stroke is the second leading cause of mortality and disability in the world. Post-stroke rehabilitation is still unsatisfactory in clinics, which brings giant pains and economic burdens to stroke patients. In this study, an injectable hydrogel where tannic acid (TA) acts as not only a building block but a therapeutic drug was developed for post-stroke rehabilitation. Methods TA is used as a building block to form an injectable hydrogel (TA-gel) with carboxymethyl chitosan (CMCS) by multivalent hydrogen bonds. The morphology, rheological property, and TA release behavior of the hydrogel were characterized. The abilities of the TA-gel to modulate microglia (BV2 cells) polarization and subsequently enhance the neuroplasticity of neuro cells (N2A cells) were assessed in vitro. The TA-gel was injected into the cavity of the stroke mouse for the evaluation of motor function recovery, microglial polarization, and neuroplasticity in vivo. The investigation of the molecular pathway through which TA modulates microglia polarization was also explored both in vitro and in vivo. Results The TA-gel exhibited a sustainable released behavior of TA. It can suppress the expression of CD16 and IL-1β, and upgrade the expression of CD206 and TGF-β in the oxygen and glucose-deprived (OGD) BV2 cell, indicating the regulation of OGD BV2 cells to anti-inflammatory phenotype in vitro. It further shows the decrease of synaptophysin and PSD95 in the OGD N2a cells is effectively recovered by the anti-inflammatory BV2 cells. Furthermore, it showed the TA-gel can decrease the CD16/iNOS expression, and increase the CD206 expression in the peri-infarct area of stroke mice, implying anti-inflammatory polarization of microglia in vivo. The colocalization of PSD95 and Vglut-1 stains as well as the Golgi stain showed the enhancement of neuroplasticity by the TA-gel. Spontaneously, the TA-gel successfully recovered the motor function of stroke mice. The western blot results in vitro and in vivo suggested TA-gel regulated microglia polarization via the NF-κB pathway. Conclusion The TA gel serves as an effective brain injectable implant to treat stroke and shows promising potential to promote post-stroke rehabilitation in the clinic.
The effect of rotation on gas storage in nanoporous materials
Authors: Kosheleva Ramonna I. , Karapantsios Thodoris D. , Kostoglou Margaritis , Mitropoulos Athanasios Ch. 
Published: August 2023

Abstract
Nanoporous materials offer a promising solution for gas storage applications in various scientific and engineering domains. However, several crucial challenges need to be addressed, including adsorptive capacity, rapid loading, and controlled gas delivery. A potential approach to tackle these issues is through rotation-based methods. In this study, we investigate the impact of rotation on CO2 adsorption using activated carbon, both at the early and late stages of the adsorption process. Towards this direction, three sets of experiments were conducted; i) adsorption isotherm with rotation at each gas loading, ii) adsorption kinetics with multiple rotation performed in sequence 15 min after CO2 introduction and iii) adsorption kinetics with a single rotation after 40 h of adsorption and repetition after another 20 h. For the first two cases the comparison was performed by respective measurements without rotation while for the last case, results were compared to theoretical pseudo first order kinetic curve. Our findings demonstrate that rotation enhances the adsorptive capacity by an impressive 54%, accelerates kinetics by a factor of 3.25, and enables controllable gas delivery by adjusting the angular velocity. These results highlight rotation as a promising technique to optimize gas storage in nanoporous materials, facilitating advancements in numerous scientific and engineering applications.
Efficient ECG classification method for arrhythmia using MODWPT and adaptive incremental broad learning
Authors: Li Jia , Huang Li , Cai Dingjian , Ling Shikang , Yang Min 
Published: August 2023

Abstract

Arrhythmia, which is deffned as a problem with the rhythm or rate at which the heart operates, is highly imperceptible yet insidious and can result in severe complications. In this study, in order to develop and evaluate an efffcient electrocardiogram (ECG) classiffcation method for arrhythmia, we propose a combination of maximum overlap discrete wavelet packet transform (MODWPT) and adaptive incremental broad learning for arrhythmia detection. In the proposed method, ECG signal is ffrst denoised and segmented. Then the energy spectrum features extracted by MODWPT is combined with morphological features and rhythmic features, are used as the input of Ada-IBL. Finally, the MIT-BIH arrhythmia database based on the AAMI ECAR1987 is used to compare the results. The accuracies for ventricular and supraventricular ectopic beats are 97.2% and 99.9%, respectively, indicating that the proposed method is superior to the existing methods. By compared with the convolutional neural network, Ada-IBL provides better computational efffciency and classiffcation accuracy. It requires only 0.31 s to achieve 99.15% classiffcation accuracy on the testing set while the training time is less than 2.4 s. Ada-IBL can well avoid the limitation of selecting parameters by experience, showing the ability of equilibrating time cost and accuracy.
Seasonal and spatial variations in macrozooplankton quality as food for fish in a SW Atlantic Ocean gulf – the role of lipids and fatty acids
Authors: Temperoni Brenda , Naveira Rocío Isla , Turina Yanina , Massa Agueda Elena 
Published: August 2023

Abstract

Macrozooplankton nutritional quality is essential information to understand the energy and matter fluxes in marine food webs and their value as prey for fish. Lipid-derived energy density (ED; kJ g− 1) and polyunsaturated fatty acids content (PUFA; µg mg− 1 total lipids) of Euphausia spp. and Munida gregaria were studied along a seasonal cycle (September 2016- November 2016-January 2017), and related with their size and the spatial environmental variability (satellite chlorophyll-a (chl-a) concentration and frontal structures) of the San Jorge Gulf (45°–47°S, 65°30′-67°30ʹW; Argentinean Continental Shelf, SW Atlantic Ocean). This gulf is a key nursery and feeding ground for many commercially and ecologically relevant fish species (e.g. Merluccius hubbsi). While ED was similar in both taxa, PUFA content was significantly higher in Euphausia spp. Quality indices showed seasonal and spatial variations, while the effect of size was non-significant. For both taxa, minimum ED values occurred in winter, while for Euphausia spp. the maximum was observed in summer and for M. gregaria in spring. M. gregaria ED tightly coupled with the seasonal chl-a cycle. PUFA content also varied seasonally, with an opposite trend. Spatially, higher ED and PUFA values occurred in locations with elevated chl-a concentrations. This was mostly manifested in M. gregaria indices and less evident in Euphausia spp. Results provide novel and baseline information of zooplankton, which can be applied in food web models to understand the trophic dynamics of many fish and top predators species in the Argentinean Shelf in the current global change context.
Medium Parental Resistance of FHB Enhances Mapping of Main and Epistatic QTL Effects in Spring Wheat Population
Authors: ElDoliefy Ahmed ElFatih , Anderson James , Glover Karl , Elias Elias , Ashry Hisham , ElZahaby Ibrahim , Mergoum Mohamed 
Published: August 2023

Abstract

Fusariumhead blight (FHB) is a devastating disease lowering yield and quality of wheat. Intensive use of limited allelic pools critically affects wheat resistance, while advances pathogenic alleles. This study presents new alleles raised from moderate rather than elite crosses. ‘Parshall’ has indigenous moderate resistance that was crossed with the moderate susceptible ‘Reeder’ and developed the 110 RIL population (noted as PR) that was tested across three states and years under field and greenhouse conditions. Heading- and FHB-related traits of incidence, severity, and index were assessed. A genetic map (1417cM) of SNP/DArT markers was generated. Composite interval mapping produced 45 QTL. Permutated LOD assessment identified two consistent QTL (4AL and 4BL) of type I and II resistance. Significant markers at the 4A QTL showed correlation with previous FHB resistance, while at the 4B QTL with pathogen responsive regions. The 1- and 2-D genome scan identified 16 QTL and six pairs of interacting resistance markers; respectively, with additive effects between 0.17% and 2.19%. Additionally, additive×environment interaction was between 0.04% and 3.18% confirming the high environmental effect. Three pairs of QTL with additive×additive effects between 0.15% and 0.30% were revealed by FHB epistasis. However, the additive×additive environmental interaction at these three loci was varied between 0.03% and 1%, confirming the G×E effect on FHB resistance. Our results have broadened the allelic pool of wheat resistance. PR-alleles can help breeders to develop lines mitigating epidemic environments and changeable climates, to boost yield facing hanger, food scarce, and unsecure worldwide penurious economies.
Hydrological Analysis of Stream Flow Characteristics of Huluka River, Abay Basin
Authors: Bayana Darara Dabtara , Chalchisa Adisu Fikire 
Published: August 2023

Abstract

A thorough assessment of the magnitude and regularity of a river's low flow is necessary for planning and designing water resource projects. The catastrophic consequences of water scarcity have made it increasingly clear that low flow conditions are perilous. Another critical skill is the ability to anticipate low flow at any specific riverbank. The severity of low flows is affected by the way the terrain regulates water storage and release throughout time, which in turn is impacted by the climate. However, knowledge of the magnitude and frequency of river flow is necessary for both present-day and future water resource planning. This paper concentrates on the characteristics of Huluka River flow using stream flow record from 1995 to 2016. In addition, Hydrological index computations, such as Flow duration curve and fundamental discharge indices, determined the methodologies for flow characterization. In the areas of stream flow analysis and categorization, the zero-flow index was employed. Based on this index, the classification of stream flow was established. The study's findings demonstrate that the Huluka River has behavior of perennial stream type and good ground water contribution to stream flow due to its highest Baseflow Index and low Zero Flow Index. The value provided for Zero Flow Index and Baseflow Index were 0.004% and 67%, respectively. The findings of this study offer important insights for water resource management.
Ubiquitination Degraded BMPR2 By Smurf1 Promotes Bladder Cancer Cell Growth
Authors: Su Qiongli , Huang Li , Guo Ying , Wei Long , Wu Ling , Tan Qiuhong 
Published: August 2023

Abstract
Bladder cancer (BC) is one of the most frequent malignancy cancers in the urinary system worldwide. It has high mortality because chemotherapy and surgery are only effective to very limited BC patients. Thus, developing novel treatment options has become an urgent need to improve clinical outcomes and the quality of life for BC patients. In this study, we proved that BMPR2 degraded by ubiquitin ligase Smad ubiquitination regulator 1 (Smurf1) can promote BC cell growth. Firstly, we detected the interaction relationship between Smurf1 and BMPR2 by CO-IP and bioinformatics tools. Next, transfection lentiviral vectors Plasmids or siRNA was used to over-express or knock-down for Smurf1 in BC cells. Finally, CCK-8 and colony assay were used to detect the growth and proliferation of BC cells J82 and UMUC3. The results demonstrated that Smurf1 plays an essential role in the Smurf1/BMPR2 signaling pathway in J82 and UMUC3 cell growth. Moreover, BMPR2 has a negative correlation with Smurf1 in BC. Additionally, Smurf1 specifically targets BMPR2 and promotes the BMPR2 degradation through the ubiquitin-protease pathway. In summary, Smurf1 may play an important role in the treatment of BC and may be a potential novel target for BC.