bluuebunny/crossref_metadata_2025_split huggingface dataset:
Zincophilic Design and the Electrode/Electrolyte Interface for Aqueous Zinc‐Ion Batteries: A Review
Authors: Zhao Chenyang , Zhang Yu , Gao Jiaze , Guo Zhikun , Chen Aosai , Liu Nannan , Lu Xingyuan , Zhang Xigui , Zhang Naiqing 
Published: February 2023

AbstractAqueous zinc‐ion batteries (ZIBs) are limited in energy storage by the persistent Zn dendrites, which is a major bottleneck preventing the commercial application of rechargeable aqueous zinc‐ion batteries. The dendritic problems associated with Zn metal anodes can lead to rapid performance degradation, failure and even safety risks of the battery. Zincophilicity of the electrode/electrolyte interface is critical to the stable and safe operation for aqueous ZIBs. In this review, we discuss that zincophilicity can be regulated by tuning the chemical interactions of material surface energy with zinc metal, emphasizing the importance of zincophilic design for improving the electrochemical performance of zinc electrodes. Advances in optimization strategies for the zincophilic zinc metal anode/electrolyte interface are summarized. Challenges and prospects for further exploration and balance of zincophilicity of the anode/electrolyte interface are presented, which are expected to promote in‐depth research and practical applications of advanced aqueous zinc ions.
Isolation and characterization of N‐(2‐Hydroxyethyl)hexadecanamide from Colletotrichum gloeosporioides with apoptosis‐inducing potential in breast cancer cells
Authors: Rai Nilesh , Gupta Priyamvada , Verma Ashish , Singh Santosh Kumar , Gautam Vibhav 
Published: February 2023

AbstractEndophytic fungi are a well‐established reservoir of bioactive compounds that are pharmaceutically valuable and therefore, contribute significantly to the biomedical field. The present study aims to identify the bioactive anticancer compound from ethyl acetate extract of fungal endophyte, Colletotrichum gloeosporioides associated with the leaf of the medicinal plant Oroxylum indicum. The fatty acid amide compound N‐(2‐Hydroxyethyl)hexadecanamide (Palmitoylethanolamide; PEA) was identified using antioxidant activity‐guided fractionation assisted with tandem liquid chromatography coupled with quadrupole time of flight mass spectrometry, Fourier transform‐infrared spectroscopy, time‐of‐flight mass spectrometry, and nuclear magnetic resonance. In‐Silico molecular docking analysis showed that PEA potentially docked to the active sites of apoptosis‐inducing proteins including BAX, BCL‐2, P21, and P53. Further validation was done using in vitro study that showed PEA inhibitsthe proliferation, alters nuclear morphology and attenuates the wound closure ability of MDA‐MB‐231 and MCF‐7 cells. PEA induces apoptosis via upregulating cell‐cycle arrest (P21), tumor suppression (P53), pro‐apoptotic (BAX, CASPASE‐8, and FADD) genes, and downregulating anti‐apoptotic gene BCL‐2. The upregulation of the active form of Caspase‐3 was also reported. This is the first‐ever report for the isolation of PEA from C. gloeosporioides with anticancer activity against human breast cancer cells and therefore holds great potential for future therapeutics.
Exploring the potential of CRISPR/Cas genome editing for vegetable crop improvement: An overview of challenges and approaches
Authors: Das Tuyelee , Anand Uttpal , Pal Tarun , Mandal Sayanti , Kumar Manoj , Radha  , Gopalakrishnan Abilash Valsala , Lastra José M. Pérez de la , Dey Abhijit 
Published: February 2023

AbstractVegetables provide many nutrients in the form of fiber, vitamins, and minerals, which make them an important part of our diet. Numerous biotic and abiotic stresses can affect crop growth, quality, and yield. Traditional and modern breeding strategies to improve plant traits are slow and resource intensive. Therefore, it is necessary to find new approaches for crop improvement. Clustered regularly interspaced short palindromic repeats/CRISPR associated 9 (CRISPR/Cas9) is a genome editing tool that can be used to modify targeted genes for desirable traits with greater efficiency and accuracy. By using CRISPR/Cas9 editing to precisely mutate key genes, it is possible to rapidly generate new germplasm resources for the promotion of important agronomic traits. This is made possible by the availability of whole genome sequencing data and information on the function of genes responsible for important traits. In addition, CRISPR/Cas9 systems have revolutionized agriculture, making genome editing more versatile. Currently, genome editing of vegetable crops is limited to a few vegetable varieties (tomato, sweet potato, potato, carrot, squash, eggplant, etc.) due to lack of regeneration protocols and sufficient genome sequencing data. In this article, we summarize recent studies on the application of CRISPR/Cas9 in improving vegetable trait development and the potential for future improvement.
Experimental kS estimation: A comparison of methods for Corynebacterium glutamicum from lab to microfluidic scale
Authors: Steinhoff Heiko , Finger Maurice , Osthege Michael , Golze Corinna , Schito Simone , Noack Stephan , Büchs Jochen , Grünberger Alexander 
Published: February 2023

AbstractKnowledge about the specific affinity of whole cells toward a substrate, commonly referred to as kS, is a crucial parameter for characterizing growth within bioreactors. State‐of‐the‐art methodologies measure either uptake or consumption rates at different initial substrate concentrations. Alternatively, cell dry weight or respiratory data like online oxygen and carbon dioxide transfer rates can be used to estimate kS. In this work, a recently developed substrate‐limited microfluidic single‐cell cultivation (sl‐MSCC) method is applied for the estimation of kS values under defined environmental conditions. This method is benchmarked with two alternative microtiter plate methods, namely high‐frequency biomass measurement (HFB) and substrate‐limited respiratory activity monitoring (sl‐RA). As a model system, the substrate affinity kS of Corynebacterium glutamicum ATCC 13032 regarding glucose was investigated assuming a Monod‐type growth response. A kS of <70.7 mg/L (with 95% probability) with HFB, 8.55 ± 1.38 mg/L with sl‐RA, and 2.66 ± 0.99 mg/L with sl‐MSCC was obtained. Whereas HFB and sl‐RA are suitable for a fast initial kS estimation, sl‐MSCC allows an affinity estimation by determining tD at concentrations less or equal to the kS value. Thus, sl‐MSCC lays the foundation for strain‐specific kS estimations under defined environmental conditions with additional insights into cell‐to‐cell heterogeneity.
The significance of margins in pediatric Non‐Rhabdomyosarcoma soft tissue sarcomas: Consensus on surgical margin definition harmonization from the INternational Soft Tissue SaRcoma ConsorTium (INSTRuCT)
Authors: Sparber‐Sauer Monika , Ferrari Andrea , Spunt Sheri L. , Vokuhl Christian , Casey Dana , Lautz Timothy B. , Meyer William H. , Walterhouse David O. , Pajtler Kristian W. , Alaggio Rita , Schmidt Andreas , Safwat Akmal , Timmermann Beate , Dall'Igna Patrizia , Chen Sonja , Weiss Aaron R. , Orbach Daniel 
Published: February 2023

AbstractBackgroundMargin status following surgery in children, adolescents, and young adults with soft tissue sarcomas is controversial and has been defined differently by various specialties, with definitions changing over time and by cooperative group. The International Soft Tissue Sarcoma Consortium (INSTRuCT) is a collaboration of the Children's Oncology Group (COG) Soft Tissue Sarcoma Committee, European pediatric Soft Tissue sarcoma Study Group (EpSSG), and the European Cooperative Weichteilsarkom Studiengruppe (CWS) devoted to improving patient outcomes by pooling and mining cooperative group clinical trial data.MethodsThe INSTRuCT non‐rhabdomyosarcoma soft tissue sarcoma (NRSTS) working group aimed to develop international harmonized recommendations regarding surgical margin assessment and definitions in children and adolescents with soft tissue tumors.Results and ConclusionThis review addresses accepted principles and areas of controversy, including the perspectives of surgeons, pathologists, radiation oncologists, and pediatric oncologists, to develop a framework for building common guidelines for future research.
An encoder‐decoder based CNN architecture using end to end dehaze and detection network for proper image visualization and detection
Authors: Shit Sahadeb , Das Dibyendu Kumar , Ray Dip Narayan , Roy Bappadittya 
Published: February 2023

AbstractIndustrial sectors are reinventing in automation, stability, and robustness due to the rapid development of artificial intelligence technologies, resulting in significant increases in quality and production. Visual‐based sensor networks capture various views of the surrounding environment and are used to monitor industrial and transportation sectors. However, due to unclean suspended air particles that damage the whole monitoring and transportation systems, the visual quality of the images is degraded under adverse weather conditions. This research proposed a convolutional neural network‐based image dehazing and detection approach, called end to end dehaze and detection network (EDD‐N), for proper image visualization and detection. This network is trained on real‐time hazy images that are directly used to recover dehaze images without a transmission map. EDD‐N is robust, and accuracy is higher than any other proposed model. Finally, we conducted extensive experiments using real‐time foggy images. The quantitative and qualitative evaluations of the hazy dataset verify the proposed method's superiority over other dehazing methods. Moreover, the proposed method validated real‐time object detection tasks in adverse weather conditions and improved the intelligent transportation system.
BML‐111 inhibits osteoclast differentiation by suppressing the MAPK and NF‐κB pathways, alleviating deterioration of the knee joints in a CIA rat model
Authors: Wang Lu , Su Wei , Zheng Xiaohang , Lin Wenjun , Lv Chen , Yang Shengwu , Chen Bicheng , Zhang Chunwu 
Published: February 2023

AbstractIrreversible destruction of joints is the hallmark of rheumatoid arthritis (RA). Osteoclasts are the only bone‐resorbing cells and play an important role in joint rebuilding. BML‐111 (5(S),6(R),7‐trihydroxyheptanoic acid methyl ester, C8H16O5) is a synthetic lipoxin A4 agonist with antioxidant and anti‐inflammatory properties. The present study aimed to investigate the effect of BML‐111 on osteoclasts in vivo and in vitro, to investigate its therapeutic effect on joint destruction in RA. Cell Counting Kit‐8 assay and flow cytometry were used to exclude cytotoxic effects of BML‐111 to bone marrow‐derived macrophages (BMMs). Then, osteoclasts were differentiated in vitro from BMMs by used macrophage colony‐stimulating factor and receptor activator of nuclear factor‐κB ligand, and osteoclasts were observed following tartrate‐resistant acid phosphatase staining with or without BML‐111 treatment. Meanwhile, absorption pit assay and immunofluorescence staining of the fibrous actin ring were used to observe osteoclast function. Moreover, we examined mitogen‐activated protein kinase (MAPK) and nuclear factor‐κB (NF‐κB) activation. We established collagen‐induced arthritis in a rat model and, after treatment with BML‐111, joint swelling was measured and the knee joints were processed for histology. We also examined serum and tissue for osteoclastogenesis‐related markers. BML‐111 inhibited osteoclast formation and differentiation in a time‐ and concentration‐dependent manner, and downregulated the expression levels of MAPK and NF‐κB in vitro. Meanwhile, BML‐111 effectively alleviated joint structural damage and inhibited osteoclast formation in vivo. BML‐111 inhibited osteoclast formation and differentiation in vitro and in vivo, and delayed the progression of joint destruction.
Determination of TMPRSS2‐ERG, SPOP, FOXA1, and IDH1 prostate cancer molecular subtypes in Colombian patients and their possible implications for prognosis
Authors: Montero‐Ovalle Wendy , Sanabria‐Salas María C. , Mesa‐López de Mesa Jorge , Varela‐Ramírez Rodolfo , Segura‐Moreno Yenifer Y. , Sánchez‐Villalobos Santiago A. , Nuñez‐Lemus Marcela , Serrano Martha L. 
Published: February 2023

AbstractProstate cancer (PCa) is one of cancer with of the highest incidence and mortality worldwide. Current disease prognostic markers do not differentiate aggressive from indolent PCa with sufficient certainty, and characterization by molecular subtypes has been sought to allow a better classification. TMPRSS2‐ERG, SPOP, FOXA1, and IDH1 molecular subtypes have been described, but the association of these subtypes with prognosis in PCa is unclear; their frequency in Colombian patients is also unknown. Formalin‐fixed and paraffin‐embedded samples of radical prostatectomy from 112 patients with PCa were used. The TMPRSS2‐ERG subtype was assessed with fluorescent in situ hybridization. The mutations in SPOP, FOXA1, and IDH1 in hot‐spot regions were evaluated using Sanger sequencing. Fusion was detected in 71 patients (63.4%). No statistically significant differences were found between the state of fusion and the variables analyzed. In the 41 fusion‐negative cases (36.6%), two patients (4.9%) had missense mutations in SPOP (p.F102C and p.F133L), representing a 1.8% of the overall cohort. The low frequency of this subtype in Colombians could be explained by the reported variability in the frequency of these mutations according to the population (5%–20%). No mutations were found in FOXA1 in the cases analyzed. The synonym SNP rs11554137 IDH1105GGT was found in tumor tissue but not in the normal tissue in one case. A larger cohort of Colombian PCa patients is needed for future studies to validate these findings and gain a better understanding of the molecular profile of this cancer in our population and if there are any differences by Colombian regions.
HypERlnc attenuates angiotensin II‐induced cardiomyocyte hypertrophy via promoting SIRT1 SUMOylation‐mediated activation of PGC‐1α/PPARα pathway in AC16 cells
Authors: Yue Lijuan , Sheng Siqi , Yuan Meng , Lu Jing , Li Tianyu , Shi Yuanqi , Dong Zengxiang 
Published: February 2023

AbstractCardiac hypertrophy is a well‐established risk factor for cardiovascular mortality worldwide. According to a recent study, hypoxia‐induced endoplasmic reticulum stress regulating long noncoding RNA (HypERlnc) is significantly reduced in the left ventricular myocardium of heart failure (HF) patients compared with healthy controls. However, the effect of HypERlnc on hypertrophy is unclear. In this study, the expression level of HypERlnc in serum of patients with chronic HF was analyzed. Moreover, the cardioprotective effect and mechanism of HypERlnc against cardiomyocyte hypertrophy were explored. Here, the level of HypERlnc expression was reduced in serum of patients with HF and in Angiotensin II (Ang II)‐stimulated AC16 cells. HypERlnc overexpression could reduce cell size and inhibit expression of hypertrophy genes (ANP, BNP, and β‐MHC) in the Ang II‐induced cardiomyocyte hypertrophy. Meanwhile, HypERlnc could improve the Ang II‐induced energy metabolism dysfunction and mitochondrial damage via upregulating PGC‐1α/PPARα signaling pathway. Furthermore, it is found that SIRT1 SUMOylation mediated the HypERlnc‐induced inhibition of cardiomyocyte hypertrophy and the improvement of energy metabolism. Taken together, this study suggests that HypERlnc suppresses cardiomyocyte hypertrophy and energy metabolism dysfunction via enhancing SUMOylation of SIRT1 protein. HypERlnc is a potential novel molecular target for preventing and treating pathological cardiac hypertrophy.
Society of thoracic surgeons risk score and left atrial pressure for predicting clinical outcomes among transcatheter mitral edge‐to‐edge repair patients
Authors: Natanzon Sharon Shalom , Koseki Keita , Kaewkes Danon , Koren Ofir , Patel Vivek , Shechter Alon , Fardman Alexander , Nakamura Mamoo , Chakravarty Tarun , Makkar Raj 
Published: February 2023

AbstractBackgroundLimited data exist regarding the performance of the Society of Thoracic Surgeons (STS) risk score among transcatheter mitral edge‐to‐edge repair (TEER) patients.ObjectiveEvaluate STS score accuracy, and the incremental value of post‐procedural left atrial pressure (LAP).MethodsA retrospective analysis of TEER patients between 2013 and 2020. Patients were allocated into 3 groups: high (≥8% [n = 298, 31%]), intermediate (4%−8% [n = 318, 33%]), and low (<4% [n = 344, 36%]). Primary outcomes included 1‐year mortality or cardiovascular hospitalizations. Cox proportional hazards regression modeling was used to determine the hazard ratio of the primary outcome, and STS score accuracy was assessed by receiver operating characteristic. A spline curve was used to display the relationship between LAP and the primary endpoint. Continuous net reclassification improvement (NRI) was used to determine the incremental value of LAP.ResultsWe included 960 patients, primarily elderly (79 [70−85]), with a median STS risk of 5.6 (3−9). High‐risk patients were older (83 [75−89], 81 [74−87], 72 [64−79], p < 0.001), and had more comorbidities compared to intermediate and low‐risk groups. Upon Cox regression, STS score (high vs. low: HR 2.5 [1.7−3.8]; Intermediate vs. low: HR 1.8 [1.2−2.7] and LAP HR 1.03 [1.01−1.06], p = 0.007) were associated with the outcome. C statistics analysis revealed low accuracy of the STS score (AUC‐0.61 [0.58−0.65, p < 0.001]). Continuous NRI analysis indicated an improvement in risk prediction of 17% (6.9−26.2), p < 0.001.ConclusionSTS risk score has low accuracy in predicting clinical outcomes after TEER. Adding LAP measurements can improve reclassification and identify those prone to adverse outcomes.
Efficacy of a new generation intracoronary optical coherence tomography imaging system with fast pullback
Authors: Nishi Takeshi , Kume Teruyoshi , Yamada Ryotaro , Koto Satsohi , Sasahira Yoshitaka , Okamoto Hiroshi , Tamada Tomoko , Koyama Terumasa , Imai Koichiro , Neishi Yoji , Ughi Giovanni J. , Uemura Shiro 
Published: February 2023

AbstractObjectivesWe sought to investigate whether a novel, fast‐pullback, high‐frequency optical coherence tomography (HF‐OCT) imaging system enables data acquisition with a reduced amount of contrast agents while retaining the same qualitative and quantitative lesion assessment to conventional OCT.BackgroundThe increased amount of administered contrast agents is a major concern when performing intracoronary OCT.MethodsThe present study is a single‐center, prospective, observational study including 10 patients with stable coronary artery disease. A total of 28 individual coronary arteries were assessed by both fast‐pullback HF‐OCT and by conventional OCT.ResultsThe contrast volume used in each OCT run for the HF‐OCT system was significantly lower than for the conventional OCT system (5.0 ± 0.0 mL vs. 7.8 ± 0.7 mL, respectively, with a mean difference of −2.84 [95% confidence interval [CI]: −3.10 to −2.58]). No significant difference was found in the median value of the clear image length between the two OCT systems (74 mm [interquartile range [IQR]; 63, 81], 74 mm [IQR; 71, 75], p = 0.89). Fast‐pullback HF‐OCT showed comparable measurements to conventional OCT, including minimum lumen area (3.27 ± 1.53 mm2 vs. 3.21 ± 1.53 mm2, p = 0.27), proximal reference area (7.03 ± 2.28 mm2 vs. 7.03 ± 2.34 mm2, p = 0.96), and distal reference area (5.93 ± 1.96 mm2 vs. 6.03 ± 2.02 mm2, p = 0.23). Qualitative OCT findings were comparable between the fast‐pullback HF‐OCT runs and conventional OCT with respect to identifying lipid‐rich plaques, calcifications, layered plaques, macrophages, and cholesterol crystals.ConclusionWith the fast pullback function of a novel HF‐OCT imaging system, we acquired OCT images using a significantly lower amount of contrast volume while retaining a comparable qualitative and quantitative lesion assessment to conventional OCT.
3D Printing‐guided endovascular repair of enormous twisted thoracoabdominal aortic aneurysm with branch stenosis and occlusion
Authors: Li Wen‐Dong , Keyoumu Reyaguli , Wang Chao , Liu Zhao 
Published: February 2023

AbstractA 67‐year‐old male patient was admitted with an enormous twisted thoracoabdominal aortic aneurysm (TAAA) with multiple branch arteries stenosis and occlusion. Three‐dimensional (3D) printing technology combined with mechanics was used for developing a transparent model of lesion to simulate the segment of diseased aorta. A stent graft was deployed in the 3D model to make a physician‐modified stent graft (PMSGs) on table. The locations of the opening of branches were marked twice during operation. The PMSG was successfully deployed during the surgery and repaired the TAAA, with no endoleak and all the branched arteries patency in follow‐up. This technique could offer precision individualized therapy and could simplify the procedure process greatly.
Evaluation of the Dry Deposition of Particles Emitted by Biomass Combustion
Authors: Costa Maria Angélica Martins , Sampaio de Mello Bruna , dos Santos Olimpio Dias Airton , Donato Gabriela , Dussan Kelly Johana , Sarti Arnaldo 
Published: February 2023

AbstractThe dry deposition flux of particles smaller than 2.5 µm was investigated considering agricultural and urban regions. The concentrations, size distributions, and chemical characterization of particulate matter (PM) were evaluated and related to emission sources. The concentrations of fine PM were associated to the highest number of wildfire outbreaks. The results of the PM samples combined with the analyses of the air mass trajectories evidenced that the transport of this pollutant may have come from regions with industries that use biomass as fuel. The predominant water‐soluble ions in coarse and fine particulate were nitrate, potassium, calcium, magnesium, potassium, sulfate, and nitrate, respectively. The deposition fluxes of potassium, nitrate, and sulfate ions were higher during the sugarcane harvest than during the off‐season.
Solid‐Phase Extraction of the Aromatic By‐Products Obtained by Fast Pyrolysis of Pretreated Lignocellulose
Authors: Meile Kristine , Jermolajeva Anastasija , Volkova Evita , Zhurinsh Aivars 
Published: February 2023

AbstractPyrolysis is a recognized technology for lignocellulose conversion into bio‐based fuels, materials, and chemicals. In this work, pyrolysis liquids were obtained by fast pyrolysis of wood after pretreatment with diluted sulfuric acid. The pyrolysis liquids were separated by solid‐phase extraction with an anion exchange resin Lewatit VPOC 1074 as the stationary phase. The aromatic constituents of the pyrolysis liquids were adsorbed on the resin, while the sugar derivative fraction was eluted with water. Different desorption solvents were tested for batch and continuous elution of phenol derivatives. Most promising results were obtained for selective separation of phenol carboxylic acids by changing the ratio of water, methanol, and acetic acid in the solid‐phase extraction solvent system.
Hydrogen Sensor to Monitor the Conditions in the Primary Circuit of a Nuclear Reactor
Authors: Moucha Tomáš , Linek Václav , Bouřa Adam , Žák Adrián 
Published: February 2023

AbstractA hydrogen concentration measurement device is developed for nuclear reactor primary circuits water. Despite the necessity to monitor hydrogen, only one producer offers a really selective hydrogen sensor on the world market because of the complexity of the device preparation when long‐term signal stability is required. Therefore, the Mass Transfer Laboratory at UCT Prague shares the experience gained during the sensor development. The results of the amperometric hydrogen sensor laboratory tests are presented, which demonstrate how to improve the sensor durability. An operational test in Dukovany nuclear power plant confirmed a significant improvement of the latest sensor version and showed that a simple principle could be realized in the form of a reliable device for industrial measurements.