bluuebunny/crossref_metadata_2025_split huggingface dataset:
Assessment of Anti-Biofilm and Anti-quorum Sensing Activities of Petroleum Oil-Eating Pseudomonas aeruginosa
Authors: ERDEM Belgin , KIRAY Esin , YALÇIN Dilek , ERKAYA İlkay AÇIKGÖZ 
Published: February 2023

Abstract

The purpose of this research is to recognise antiquorum sensing and antibiofilm activity against biofilm-forming bacteria (Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Listeria monocytogenes, Enterobacter aerogenes, and Bacillus cereus) and virulence factors in Pseudomonas extracts isolated from petroleum-oil contaminated side. The extracts were tested for anti-QS activity on Chromobacterium violaceum ATCC 12472 in double-fold dilutions of 50, 25, 12.5 and 6.25 mg/mL. The inhibition of violecein production from extracts at 50, 25, 12.5 and 6.25 mg/mL dilutions were the best determined between 30.2% and 72.5% at the dilution of 50 mg/mL. The crude extracts from the P9 (78%), P12 (88%) and P13 (75%) isolates, B. cereus 709 Roma have the best biofilm inhibition activity. At the same time, the crude extracts of P3, P8 and P13 isolate, E. aerogenes (91%), (85%) and (70%) and showed the highest biofilm inhibition activity, respectively. Multidrug-resistant bacteria commonly use cell-to-cell communication leading to biofilm formation as one of the mechanisms of resistance development. Quorum sensing inhibition (QSI) is an effective approach to prevent biofilm formation. A gram-negative bacterium, 11 P. aeruginosa, was isolated from soil contaminated with petroleum oil. Bacterial crude extracts showed QSI activity in the biosensor plate containing the reference strain C. violaceum ATCC 12742. A reduction of approximately 98% in violacein production was detected with the reference strain C. violaceum ATCC 12742. The aim of this research is to screen anti-quorum sensing activity and inhibited biofilm activity of Pseudomonas aeruginosa isolated from soil contaminated with petroleum oil and to measure the antibiofilm activities by biofilm forming bacteria (S. aureus, E. coli, P. aeruginosa, E. aerogenes, L. monocytogenes and B. cereus 709 Roma).
Preoperative virtual iliosacral screw combined with individualized 3D printing navigation template for accurate treatment of posterior pelvic ring injury in a clinical study
Authors: Zhao Peishuai , Wang Xiaopan , Guan Jianzhong , Chen Xiaotian , Li Renjie , Wu Min 
Published: February 2023

Abstract
Purpose: This research introduced a novel approach that combines a Three-dimensional(3D) printed guide-plate with preoperative virtual iliosacral screws to guide the insertion of intraoperative screws, and compares its effectiveness and safety to traditional surgical procedures.
Methods：59 patients in our hospital were randomized to a guide-plate group (n=30) or a traditional surgical group (n=29) after receiving percutaneous iliosacrals for unstable posterior pelvic ring injuries. Gender, age, injury mechanism, sacrum type, fracture type, time of each screw placement, radiation exposure time of each screw, and postoperative screw classification are all factors to take into account.
Results: In 59 patients, a total of 83 iliosacral screw were implanted, including 43 screws in the guide-plate group (37 in S1 and six in S2) and 40 screws in the traditional surgery group (34 in S1 and six in S2). The screw dislocation rate of patients in the guide-plate group (2.3%) was considerably lower than that of the traditional surgery group (15.0%); the radiation exposure duration for each screw in the guide-plate group (0.4±0.1min) was lower than that of the traditional operation group (0.9±0.2min). The screw dislocation rate and radiation exposure time were statistically significant (P<0.05). The time it took to place each screw in the traditional surgery group (25.9±3.8min) was longer than in the guide-plate group (19.2±2.8min), although this difference was not statistically significant (p>0.05).
Conclusion: Preoperative virtual screw technology combined with 3D guide technology can accurately place iliosacral screws, introducing a new option for percutaneous iliosacral screw placement.
Gallbladder Cancer Incidentally Found at Cholecystectomy: Perioperative Risk Factors
Authors: Olecki Elizabeth J. , Mayhew Mackenzie M. , Perez-Holguin Rolfy , Wong William G. , Stahl Kelly A. , Peng June S. , Dixon Matthew E. , Gusani Niraj J. 
Published: February 2023

Abstract
Purpose: Risk factors of Gallbladder Cancer (GBC) are not well-defined resulting in greater than 60% of GBCs being diagnosed incidentally following cholecystectomy performed for presumed benign indications. As most localized GBC require more extensive oncologic surgery beyond cholecystectomy, this study aims to examine factors associated with incidentally found GBC to improve preoperative and intraoperative diagnosis.
Methods: The American College of Surgeons National Surgical Quality Improvement Program Database from 2007-2017 was used to identify cholecystectomies performed with and without a final diagnosis of GBC. Univariate and multivariable logistic regression were used to compare demographic, intraoperative, and postoperative characteristics among those with and without a diagnosis of GBC.
Results: The incidence of GBC was observed to be 0.11% (441/403,443). Preoperative factors associated with risk of GBC included age >60 (OR 6.51, p<.001), female sex (OR 1.75, p<.001), history of weight loss (2.58, p<.001), and elevated preoperative alkaline phosphatase level (OR 1.67, p=.001). Open approach was associated with 7 times increased risk of GBC compared to laparoscopic approach (OR 7.33, p<.001). In addition to preoperative factors and surgical approach, longer mean operative times (127 minutes vs 70.7 minutes, p<.001) were significantly associated with increased risk of GBC compared to benign final pathology.
Conclusion: This study demonstrates that those with incidentally discovered GBC at cholecystectomy are unique from those undergoing cholecystectomy for benign indications. By identifying predictors of GBC, surgeons can choose high risk individuals for pre-operative oncologic evaluation and consider better tools for identifying GBC such as intraoperative frozen pathology.
A novel pancreaticoduodenectomy reconstruction for a patient with Roux-en-Y hepaticojejunostomy after radical extended left hepatectomy for perihilar cholangiocarcinoma
Authors: Ohwada Susumu , Sasaki Kazunari , Nakano Yuhei , Kawate Susumu , Kashima Yasushige , Matsuyama Hideki , Iijima Misa , Noda Naohiro , Hoshino Masato , Maruyama Hideki , Okauchi Kenzo 
Published: February 2023

Abstract
Background:
Metachronous cholangiocarcinoma in the remnant distal bile duct (BD) has been reported after the resection of perihilar cholangiocarcinoma. A pancreatoduodenectomy (PD) after radically extended hepatectomy with Roux-en-Y hepaticojejunostomy has significant surgical complexity in in both extensive adhesiolysis and digestive tract reconstruction. Herein, we reported a novel method of digestive tract reconstruction using the Y limb of pre-existing Roux-en-Y hepaticojejunostomy for pancreaticoduodenostomy and, subsequently, the Roux jejunal limb for gastrojejunostomy.
Case presentation:
A 74-year-old male developed distal BD cholangiocarcinoma six months after extended left hepatectomy for perihilar cholangiocarcinoma and subsequent adjuvant chemotherapy. The patient underwent extensive adhesiolysis followed by PD. His digestive tract was reconstructed by a novel method of PD reconstruction: the Y limb of the pre-existing Roux-en-Y hepaticojejunostomy was used for pancreaticoduodenostomy, and the Roux jejunal limb was used for the gastrojejunostomy. The tumor pathology demonstrated histologically stage Ib biliary duct adenocarcinoma with fibromuscular invasion.There were no complications of a pancreaticodeodenostomy fistula.
Conclusions:
This novel method of PD reconstruction for patients with Roux-en-Y hepaticojejunostomy could take advantage of the previous surgically altered anatomy and avoid further adhesiolysis for obtaining the adequate length of a new jejunal loop for pancreaticojejunostomy and gastrojejunostomy. This can be an optional PD reconstruction for patients with Roux-en-Y hepaticojejunostomy in cases of early-stage bile duct cancer and/or less likely to have lymph node metastasis.
Male partners’ involvement and its associated factors in promoting skilled birth use of wives in Dire Dawa Administration, eastern Ethiopia
Authors: Mohammed Aminu , Hailu Mickiale , Tadesse Daniel , Bireda Asma , Manaye Yibekal 
Published: February 2023

Abstract
Background
Male partner involvement in promoting skilled delivery is a process of social and behavioral change that men must undergo in order to prevent obstetric complications during childbirth. But this lacks information, and therefore, it was intended to be investigated in the study area.
Methods
A community-based cross-sectional study was conducted in the Dire Dawa administration, Ethiopia, from July 1 to 30, 2022, among male partners chosen using a systematic sampling technique. Data were collected through face-to-face interviews using a structured questionnaire, entered and cleaned by Epi DATA (Version 3.1), and analyzed using SPSS (Version 22). A P-value of 0.25 at bivariate to select variables for multivariate and 0.05 at multivariate with 95% confidence intervals was considered statistically significant.
Results
A total of 610 male partners participated, and male partners’ involvement in promoting skilled birth attendance was 51.8% (95% CI: 47.5–55.4%). The predictors were urban residence (AOR = 2.55, 95%CI: 1.75–3.73), diploma and above level of education (AOR = 2.00, 95%CI: 1.10–3.74), the perceived importance of skilled birth attendance (AOR = 1.98, 95%CI: 1.30–3.00), an antenatal care visit (AOR = 1.79, 95%CI: 1.04–3.07), and skilled birth attendance (AOR = 6.20, 95%CI: 3.4–11.33) with accompanying experiences.
Conclusion
Male partners' involvement in promoting their partners’ skilled birth attendance is moderate compared to other studies in Ethiopia. Stakeholders are recommended to use combined efforts to strengthen men's involvement and create continuous awareness about skilled birth utilization.
Black phosphorus quantum dots conjugated with dual antibodies improve tumor infiltrating CD8 + T cell activation and cancer immunotherapy
Authors: Liu Zhaoyuan , Li Wenting , Xue Ping , Jiang Xiaofeng 
Published: February 2023

Abstract
Tumor-infiltrating lymphocyte(TIL) therapy, an immunotherapy that uses CD8+T as the main killer cells isolated from the patient's tumor tissue to treat the patient's tumor, has shown efficacy in solid tumors. In the tumor microenvironment, effector CD8+T cells enter a state of impaired function known as T cell exhaustion. Therefore, how to improve their activity and rapid expansion in vitro is the key problem to be solved in TIL therapy. Combination immunotherapy has recently emerged as a potent cancer treatment strategy. And nanomaterials have shown great application value in the field of immunotherapy. We achieved optimal activation and amplification of CD8+T cells by combining organic nanomaterial black phosphorus quantum dots(BPQDs) with immune checkpoint inhibitory molecules PD-1 and LAG3. CD8+T cells enhanced cytotoxic function through a series of biomarkers (TNF-a and IFN-γ secretion) and targeted cell-killing measurements in vitro. It was related to the increase of CD8+T cells, and the increased function was more than the number of cells increased, indicating that the function of CD8+T cells was also enhanced. This work demonstrates a novel strategy for using organic nanotechnology to improve effective expansion and efficacy of tumor-infiltrating lymphocytes.
Discovering the Evolution Structure of Literature
Authors: Wu Siyuan , U Leong Hou 
Published: February 2023

Abstract
A common task in research work is to survey research work in bioliographic databases. Scientists are finding the survey task notably difficult as the volume of the databases has been increased considerably over the past few decades. Making a good use of a survey paper can vastly lower the difficulty but there may be no such paper existed in some emerging research topics. In this work, we propose a novel Literature Evolution Discovery (LED) process that aims to provide an explainable evolution structure of literature. The explain- ability is based on co-citation analysis and latent relationship extraction, which are done by Steiner tree and context-consistent factor graph model, respectively. The experiments show the superiority of our context-consistent factor graph model, compared with the state-of-the-art baselines. Our case studies and visualization results demonstrate the effectiveness and interpretability of our proposed algorithms in practice.
Fine Grain Algorithm Parallelization on a Hybrid Control-flow and Dataflow Processor
Authors: Korolija Nenad , Furht Borko , Milutinović Veljko 
Published: February 2023

Abstract
The execution time of a high performance computing algorithm depends on multiple factors: the algorithm scalability, the chosen hardware, the communication speed between processing elements, etc. This work is based on a hybrid processor consisting of both the control-flow and dataflow hardware, where the control-flow hardware includes manycore architecture. The algorithm decomposition into portions suitable for different architectures is presented. The results are presented by decomposing the Lattice-Boltzmann method implemented for both control-flow and dataflow hardware. The total acceleration factor of the decomposed Lattice-Boltzmann method comparing to the execution time using the control-flow and the dataflow hardware is obtained by the analysis based on the speed of the communication between both hardware equal to the speed of shared cache memories. Results indicate the advantage of using the proposed hybrid architecture and the capability of accelerating considerably even the suitable for dataflow architectures. The analysis of the problem size needed to justify the utilization of control-flow hardware is also presented. The main benefit is in accelerating algorithms for which only some algorithm portions are suitable for the dataflow hardware.
Candidate oncogene placenta specific 8 affect cell growth and cell migration in non- small cell lung cancers
Authors: Zhou Mei-Lin , Ma Jin-Ni , Xu Xin , Gao Xin-Yao , Wang Hai-Xia , Shen Jinhua , Xue Lu 
Published: February 2023

Abstract
Background
As a candidate oncogene, PLAC8 participate in genesis and progression of various tumors. However, the role of PLAC8 in lung cancer (LC) especially non-small cell lung cancer (NSCLC) is still limited.
Methods
We performed Tissue microarray analysis (TMA) and Real-Time PCR (RT-PCR) to detect the expression levels of PLAC8 in LC tissues and cell lines, respectively. Then a series of cellular experiments focusing on cell proliferation, cell cycle, cell motility were conducted to identified the role of PLAC8 in NSCLC-derived cell lines H1299 and A549.
Results
TMA and RT-PCR showed that PLAC8 played complicated even opposite roles in different LCs. Further cellular experiments confirmed that PLAC8 could promote cell viability, alter cell cycle, and accelerate cell mobility via regulation of cell cyclins or cadherins, respectively.
Conclusions
Our study indicated that PLAC8 might participate in LC especially NSCLC progression. Our study also shed new light on the potential role of PLAC8 as a therapeutic biomarker in NSCLC.
Outcomes of preoperative chemoradiotherapy after complete smoking cessation followed by total mesorectal excision with selective lateral pelvic lymph node dissection for locally advanced low rectal cancer
Authors: Inoue Akira , Kagawa Yoshinori , Nishizawa Yujiro , Komori Takamichi , Shimamoto Shigetoshi , Komatsu Hisateru , Miyazaki Yasuhiro , Tomokuni Akira , Motoori Masaaki , Fujitani Kazumasa 
Published: February 2023

Abstract
Purpose: Outcomes of patients with rectal cancer who underwent preoperative chemoradiotherapy after complete smoking cessation are unknown. We evaluated the outcomes of patients with locally advanced low rectal cancer who underwent combined preoperative chemoradiotherapy after complete smoking cessation and total mesorectal excision with selective lateral lymph node dissection. Predictive factors associated with response to preoperative chemoradiotherapy were investigated.
Methods: In this retrospective study at a tertiary medical center, 160 patients with locally advanced low rectal cancer who underwent surgical resection during January 2014–December 2019 were enrolled; data of 28 with stage II or III rectal cancer who underwent preoperative chemoradiotherapy and surgery were analyzed. Before chemoradiotherapy, a Smokerlyzer was used to measure expiratory carbon monoxide and monitor smoking cessation. Pathologic response rate and oncological outcomes were analyzed.
Results: No major post-surgical complications occurred. Pathologic complete response was achieved in 8/28 patients (28.6%). Three-year recurrence-free and overall survival rates were 81.4% and 92.4%, respectively. No patient developed local recurrence in lateral pelvic lymph nodes. Survival did not differ significantly between patients with and without pathologic complete response. In univariate and multivariate analyses, pretreatment hemoglobin (>13.0) [hazard ratio: 7.00 (95% confidence interval, 1.090–45.20); p=0.0408] and clinically negative N stage before treatment [hazard ratio: 18.9 (95% confidence interval, 1.63–218.0); p=0.0187] were significantly associated with pathologic complete response.
Conclusion: Preoperative chemoradiotherapy with complete smoking cessation followed by total mesorectal excision with selective lateral lymph node dissection was safely performed and resulted in high pathologic complete response rates and favorable survival outcomes.
Patient Satisfaction study in Outpatient setting in Tertiary Academic Hospital Patient Satisfaction in Outpatient Clinics
Authors: Abaalkhayl Mohammed , Abukraym Nader , Al-Najjar HANIHani 
Published: February 2023

Abstract
Purpose: To analyze and assess of satisfaction to the quality and performance of services provided in the outpatients Clinics of Tertiary Academic Care Hospital, and explore what is the factors affecting on it.
Methods: In a cross-sectional study at university hospitals in the Qassim region of Saudi Arabia, 476 outpatient online surveys were completed. The main content of the study was the basic characteristics of the patients. Statistical description, to identify influencing factors, chi-square analysis was utilized.
Results: The survey found that total outpatient satisfaction was 76.69±25.17, and the higher satisfaction factor scores were hospital facilities, Attitude and corporation of pharmacy staffs, language and communication of the physician, and registration process. The factors with lower scores were ease of finding OPD clinic, ease to find outpatients location, availability of prescribed medication.
Conclusion: For the continuation of the outpatient service process, patients with various educational levels, marital status, and first-time hospital visitors may receive special attention when receiving outpatient care.
The correlation between proline/P5C cycle and the response to avirulent pathogen infection in Arabidopsis
Authors: Zhang Yueqin , Ying Wenhan , Yang Rongchao , Cai Yuanyuan , Hua Xuejun 
Published: February 2023

Abstract
Proline/P5C cycle between mitochondria and cytosol, play important roles in energy supply and ROS generation in mammalian cell. Recently, in plant, proline-dependent ROS via proline/P5C cycle was proposed to be involved in hypersensitive reaction during plant response to avirulent pathogens. However, much remains to be elucidated about the regulation of proline/P5C cycle upon pathogen infection. Here, we reported the isolation and characterization of an Arabidopsis mutant proline resistance 2 (pre2), with a single dominant mutation in a single gene. Our results showed that the proline resistance phenotype of pre2 is not due to decreased intracellular proline content, when treated with exogenous proline. Upon proline treatment, pre2 showed reduced induction of PDH transcript level and enhanced induction of that of P5CDH, accompanied by lower level of mitochondrial ROS, suggesting an attenuated proline/P5C cycle activity. Proline-induced SA signaling was also less activated in pre2, as evidenced reduced free SA content and PR1 transcript level, compared to the WT. On the other hand, SA activation on the proline/P5C activity is to a lesser extent in pre2 than in WT. Significantly, pre2 demonstrated increased susceptibility to infection by avirulent pathogen Pst. DC3000 (avrRps4), accompanied also by lesser induced proline/P5C cycle activity by the pathogen. Our results indicated that there is a correlation between proline/P5C cycle and plant response to avirulent pathogen.
Assessing the risk of hypoglycemia secondary to propranolol therapy for the treatment of supraventricular tachycardia in infants
Authors: Parker Noah , Flowers Rhiannon , Vickery Kristen , Stolfi Adrienne , Bugnitz Christopher 
Published: February 2023

Abstract
Objective
Supraventricular tachycardia (SVT) is the most common arrythmia among infants. Prevention of SVT is frequently managed through propranolol therapy. Hypoglycemia is a known adverse effect of propranolol therapy, but little research has been done on the incidence and risk of hypoglycemia in treatment of SVT in infants with propranolol. This study attempts to offer insight into the risk of hypoglycemia associated with propranolol therapy when treating infantile SVT to help inform future glucose screening guidelines.
Methods
We conducted a retrospective chart review of infants treated with propranolol in our hospital system. Inclusion criteria were infants < 1 year of age who received propranolol for the treatment of SVT. A total of 63 patients were identified. Data was collected on sex, age, race, diagnosis, gestational age, nutrition source (Total Parenteral Nutrition (TPN) vs oral), weight (kg), weight for length (kg/cm), propranolol dose (mg/kg/day), comorbidities, and whether or not a hypoglycemic event was identified (< 60 mg/dL).
Results
Hypoglycemic events were identified in 9/63 (14.3%) patients. Of the patients with hypoglycemic events, 8/9 (88.9%) had comorbid conditions. Patients with hypoglycemic events had significantly lower weight and propranolol dose than those without hypoglycemic events. Weight for length also tended to increase risk for hypoglycemic events.
Conclusions
The high incidence of comorbid conditions in the patients who had hypoglycemic events suggests that hypoglycemic monitoring may only be necessary in patients with conditions predisposing to hypoglycemia.