bluuebunny/crossref_metadata_2025_split huggingface dataset:
Связь оценки музыкальных способностей и показателей регуляторных функций детей, посещающих музыкальные занятия
Authors: Долгих Александра Г. , Баянова Лариса Ф. , Шатская Арина Н. , Якушина Анастасия А. 
Published: February 2023

Введение. Целью данной работы было изучение согласованности оценок музыкальных способностей детей педагогами с развитием их регуляторных функций. Кроме того, в рамках исследования проводилась первичная апробация опросника для оценки музыкальных способностей детей. Новизна исследования заключается, во-первых, в разработке и использовании нового инструмента для оценки музыкальных способностей детей, основанного на концепции Б. М. Теплова, а во-вторых, в демонстрации того, что не только музыкальные занятия влияют на показатели развития регуляторных функций детей, но и некоторые из регуляторных функций также вносят вклад в музыкальные способности детей. Методы. В исследовании приняли участие 61 ребенок, в возрасте 5–6 лет, которые несколько раз в неделю дополнительно занимаются музыкой в детских школах искусств г. Москвы и г. Краснодара. В ходе диагностики с детьми проводилось несколько встреч, в рамках которых изучались такие показатели регуляторных функций, как когнитивная гибкость, рабочая память и торможение. В оценке уровня развития их музыкальных способностей приняли участие 50 преподавателей, которые заполняли специально разработанную анкету для выявления уровня развития следующих параметров музыкальных способностей детей: звуковысотный слух, чувство ритма, ладовое чувство и эмоциональная отзывчивость. Результаты. В результате было получено наличие положительной взаимосвязи между зрительной рабочей памятью и оценкой звуковысотного слуха у детей, занимающихся музыкой. Кроме того, в результате проведенного регрессионного анализа было показано, что показатель зрительной рабочей памяти детей вносит вклад в оценку таких параметров музыкальных способностей, как звуковысотный слух (R2 = 0,241, p = 0,008) и эмоциональная отзывчивость (R2 = 0,149, p = 0,043). Обсуждение результатов. Таким образом, проведенное исследование демонстрирует взаимосвязь музыкальных способностей и регуляторных функций детей, и вклад регуляторных функций в оценку музыкальных способностей детей, что обосновывает ценность систематических музыкальных занятий в дошкольном возрасте.
Hepatic Effect of 2,3,5,4'-tetrahydroxystilbene-2-O-β-D-glucoside, the Signature
Component of Traditional Chinese Medicine Heshouwu: Advances and Prospects
Authors: Wang Cheng-Yu , Hu Ying-Huan , Sun Zhen-Xiao 
Published: February 2023

Abstract:
Traditional Chinese medicine Heshouwu, named Polygoni Multiflori Radix in Pharmacopoeia of the People’s
Republic of China (PPRC, 2020), is derived from the root tuber of Polygonum multiflorum Thunb., Heshouwu
or processed Heshouwu is well known for its function in reducing lipids and nourishing the liver. However, increasing
cases of Heshouwu-induced hepatotoxicity were reported in recent years. Researchers have begun to study the
paradoxical effects of Heshouwu on the liver. 2,3,5,4'-tetrahydroxystilbene-2-O-β-D-glucoside (TSG), an abundant
functional component of Heshouwu, shows various biological activities, among which its effect on the liver is worthy
of attention. This paper reviews the current studies of TSG on hepatoprotection and hepatotoxicity, and summarizes
the doses, experimental models, effects, and mechanisms of action involved in TSG’s hepatoprotection and hepatotoxicity,
aiming to provide insight for future study of TSG and understanding the effects of Heshouwu on the liver.
Emerging evidence suggests that TSG ameliorates both pathological liver injury and chemical-induced liver injury by
modulating lipid metabolism, inhibiting the inflammatory response and oxidative stress in the liver. However, with
the reports of clinical cases of Heshouwu induced liver injury, it has been found that long-term exposure to a high
dose of TSG cause hepatocyte or hepatic tissue damage. Moreover, TSG may cause hepatotoxicity by affecting the
transport and metabolism of other possible hepatoxic compounds in Heshouwu. Studies indicate that trans-TSG can
be isomerized into cis-TSG under illumination, and cis-TSG had a less detrimental dose to liver function than trans-
TSG in LPS-treated rats. In brief, TSG has protective effects on the liver, but liver injury usually occurs under highdose
TSG or is idiosyncratic TSG-induced liver injury.
A Nutraceutical Compound Containing a Low Dose of Monacolin K,
Polymethoxyflavones, Phenolic Acids, Flavonoids, and Hydroxytyrosol
Improves HDL Functionality
Authors: Cervellati Carlo , Trentini Alessandro , Rosta Valentina , Zuliani Giovanni , Vieceli Dalla Sega Francesco , Fortini Francesca , Rizzo Paola , Cimaglia Paolo , Campo Gianluca 
Published: February 2023

Background:
In earlier studies, it has been observed that 8-week treatment with a novel
nutraceutical compound (NC) containing low monacolin K dose, polymethoxyflavones, phenolic acids,
flavonoids, and hydroxytyrosol improves lipid profile and endothelial function and reduces the level of
oxidized low-density lipoprotein (oxLDL). We hypothesize that this effect might be, at least in part,
explained by positive modulation exerted by the NC on the atheroprotective function of high-density
lipoprotein (HDL).

Aim:
This study aimed to evaluate whether the NC could influence determinants of HDL function.

Methods:
Forty-five subjects with low-moderate dyslipidaemia were enrolled and treated for 8 weeks
with the NC, followed by 4 weeks of washout. Blood samples were collected at every time point to
evaluate changes in lipid profile, endothelial function, oxLDL, and markers of HDL function, such as
the anti-oxidant activities of paraoxonase-1, glutathione peroxidase-3 (Gpx3), lipoprotein-phospholipase
A2 (Lp-PLA2), and pro-oxidant activity of myeloperoxidase (MPO).

Results:
Although the concentration of HDL-C did not change, the activity of Lp-PLA2 significantly
decreased upon treatment (-11.6%, p<0.001) and returned to baseline level 4 weeks after the end of
treatment. In contrast, Gpx3 increased after treatment (+5%, p<0.01) and remained unvaried after 4
weeks. Both MPO activity and concentration significantly decreased after the washout period (-33 and
32%, p<0.001).

Conclusion:
For the first time, it was found that the administration of an NC with beneficial effects on
lipid homeostasis also positively impacts HDL function by improving the balance between protective
and damaging determinants. Further investigation is required to corroborate our findings.
Delineating Potential de novo Therapeutics and Repurposed Drugs Against
Novel Protein LRRC15 to Treat SARS-CoV-2
Authors: Afroj Zinnia Maliha , Khademul Islam Abul Bashar Mir Md. 
Published: February 2023

Introduction:
Sudden SARS-CoV-2 pandemic disrupted global public health; hence, searching
for more effective treatments is urgently needed.

Objective:
Recently, a new host protein LRRC15 has been identified, facilitating viral attachment and
cellular invasion and hence can be a good target against SARS-CoV-2. In this study, design some potential
inhibitors against LRRC15.

Methods:
Here, we explored three strategies to find potential inhibitors against LRRC15, including the
repurposing of ACE2 inhibitors, structure-based de novo drug generation, and virtual screening of three
chemical libraries (ZINC Trial, ZINC Fragments, and Enamine HTSC).

Results:
Based on binding affinity Benazepril (-7.7 kcal/mol) was chosen as a final repurpose drug candidate,
and ten de novo drugs (-8.9 to -8.0 kcal/mol) and 100 virtually screened drugs (-11.5 to -10.7
kcal/mol) were elected for further ADMET and drug likeliness investigation. After filtering, Z131403838
and Z295568380 were chosen as final drug candidates, and de novo drugs were further optimized. Optimization,
re-docking, and pharmacokinetic analysis confirmed L-2 and L-36 as the best hit de novo drug
candidates. Furthermore, all five final drugs demonstrated stable receptor-drug complex stability in molecular
dynamics simulation.

Conclusion:
Effective treatment options are necessary to combat the SARS-CoV-2 epidemics. All the
compounds presented in this study appeared to be promising inhibitorpromising inhibitors against
LRRC15, though the future clinical investigation is needed toensure the biological effectiveness.
Demonstration of Interaction between Carbapenem Group Antibiotics and
Different Immunosuppressant Drugs by Molecular Docking
Authors: Karatas Deniz , Gonel Ataman , Koyuncu Ismail , Temiz Ebru , Egi Kadir , Durgun Mustafa , Akmese Sukru , Caglayan Murat 
Published: February 2023

Background:
It has been shown that drugs used parenterally cause errors in immunosuppressant
concentrations measured by LC-MS / MS method. It is yet unknown whether this measurement error
is due to drug-drug interaction or analytical interference.

Objective:
The aim of this study is to investigate the possible interaction and inhibition concentrations of
broad-spectrum antibiotics (ertapenem, meropenem, imipenem) with 4 different immunosuppressants
(tacrolimus, sirolimus, everolimus, cyclosporine A) by molecular docking.

Method:
The docking results of ertapenem, meropenem, and imipenem-cilastatin drugs, which are frequently
used in intensive care units and wards, were analyzed with the Autodock 4.2 program. Binding
energy levels and inhibition concentrations were recorded.

Results:
The highest binding energies of the most stable conformations, providing the best compatibility
among the active ingredients, belong to cilastatin. The interaction energy of cilastatin with sirolimus in
320 conformations was calculated as -4.08 kcal/mol. Sirolimus interacted with ertapenem at -3.43,
imipenem at -2.53, and meropenem at -3.84 kcal/mol. According to these values, the receptor, which is
the most compatible host with all ligand molecules, is sirolimus. The least interaction energy value was
calculated between cyclosporine and imipenem (-1.12 kcal / mol).

Conclusion:
Concerning the most stable conformations of models docked with Autodock tools, it has
been determined that carbapenems interact with immunosuppressants. Since the detected inhibition concentration
levels can be seen in blood samples taken immediately after carbapenem injection, immunosuppressant
measurement is recommended before the use of carbapenem in immunosuppressant monitoring
of transplant patients.
Computational Study of Lactucine and its Derivatives to Investigate its
Anti-cancerous Properties Targeting Apoptosis-inducing Protein
Authors: Arya Mamta , Tiwari Apoorv , Singh Dev Bukhsh , Taj Gohar 
Published: February 2023

Background:
Lactucine is related to the sesquiterpene lactone group of naturally occurring
compounds and has a variety of pharmacological effects including anticancer properties found in Chicory,
Wormwood, Laurus nobilis, Pyrethrum, Chamomile, etc. Lactucine has an anticancer effect which may
induce apoptosis in cancerous cells and protect other cells from getting infected.

Objective:
In this study, Lactucine and its derivatives were screened, and performed their in silico docking
study with the proteins involved in the apoptosis-inducing effect in human leukemia cancer.

Methods:
The three-dimensional structure of lactucine and its derivatives were retrieved in the SDF format.
Active sites of protein structures were determined by Sitemap. LigPrep module was used for geometrical
refining of chemical structures of lactucine and its derivatives. The protein preparation wizard of
Maestro (Schrodinger) was used for protein preparation. From the receptor-complex structure, the cocrystallized
ligands were removed from the active site position of the receptor chain. All ligands were docked
using default Glide settings for a grid centered on the ligand and structure. Flexible docking was performed
using the extra precision (XP) feature of Glide module. The best docking poses for the lactucine
and their derivatives were selected based on their docking score. The ADMET properties of lactucine 15-
oxalate have been predicted by admetSAR software.

Results:
Proteins and ligands three-dimensional structures were retrieved from PDB and Pubchem databases,
respectively. All lactucine derivatives suitably docked on the apoptosis-inducing proteins with
ample Glide scores Lactucin 15-oxalate interacts with proteins which are responsible for apoptosis with a
maximum of six H-bonds. Other types of interactions are also involved, like Pi-cation, Pi-Pi stacking, salt
bridges, and halogen bonds. Protein CDK-4 has shown the highest number of H-bond (LYS142 salt
bridges), ALA16, VAL14, ASP99, LYS35, TYR17, and ASN145) with the Lactucin 15-oxalate. ADMET
properties of lactucin 15-oxalate met with the criteria of being eligible to be a novel drug for the treatment
of human leukemia cancer. The Dock score of both the Dasatinib drug and the lactucine-15-oxalate with
the apoptosis-inducing protein stipulates that the selected ligand has equitable interaction with the target
proteins.

Conclusion:
In this study, lactucine derivatives were docked with apoptosis-inducing proteins for the
prediction of its anticancer effect. Lactucin15-oxalate has shown the highest binding affinity for the
CDK-4 target and can be used as a lead compound for cancer treatment. Glide and Dock score for docking
of lactucin 15-oxalate with CDK-4, well as the number of hydrogen bonding, is in agreement to use
this ligand for study. These in silico results are valuable to proceed with the in vitro and in vivo studies
related to the anti-cancer role of lactucin 15-oxalate.
Organosulfur Compounds in Aged Garlic Extract Ameliorate Glucose
Induced Diabetic Cardiomyopathy by Attenuating Oxidative Stress,
Cardiac Fibrosis, and Cardiac Apoptosis
Authors: Sharma Kumkum , Rani Vibha 
Published: February 2023

Background:
Diabetic cardiomyopathy has emerged as a major cause of cardiac fibrosis,
hypertrophy, diastolic dysfunction, and heart failure due to uncontrolled glucose metabolism in patients
with diabetes mellitus. However, there is still no consensus on the optimal treatment to prevent
or treat the cardiac burden associated with diabetes, which urges the development of dual antidiabetic
and cardioprotective cardiac therapy based on natural products. This study investigates the
cardiotoxic profile of glucose and the efficacy of AGE against glucose-induced cardiotoxicity in
H9c2 cardiomyocytes.

Methods:
The cellular metabolic activity of H9c2 cardiomyocytes under increasing glucose concentration
and the therapeutic efficacy of AGE were investigated using the MTT cell cytotoxicity assay.
The in vitro model was established in six groups known as 1. control, 2. cells treated with 25
μM glucose, 3. 100 μM glucose, 4. 25 μM glucose +35 μM AGE, 5. 100 μM glucose + 35 μM AGE,
and 6. 35 μM AGE. Morphological and nuclear analyses were performed using Giemsa, HE, DAPI,
and PI, respectively, whereas cell death was simultaneously assessed using the trypan blue assay. The
antioxidant potential of AGE was evaluated by DCFH-DA assay, NO, and H202 scavenging assay.
The activities of the antioxidant enzymes catalase and superoxide dismutase were also investigated.
The antiglycative potential of AGE was examined by antiglycation assays, amylase zymography, and
SDS PAGE. These results were then validated by in silico molecular docking and qRTPCR.

Results:
Hyperglycemia significantly reduced cellular metabolic activity of H9c2 cardiomyocytes,
and AGE was found to preserve cell viability approximately 2-fold by attenuating oxidative, fibrosis,
and apoptotic signaling molecules. In silico and qRTPCR studies confirmed that organosulfur
compounds target TNF-α, MAPK, TGF-β, MMP-7, and caspase-9 signaling molecules to ameliorate
glucose-induced cardiotoxicity.

Conclusion:
AGE was found to be an antidiabetic and cardioprotective natural product with exceptional
therapeutic potential for use as a novel herb-drug therapy in the treatment of diabetic cardiomyopathy
in future therapies.
Mesenchymal Stem Cells and Curcumin Effectively Mitigate Freund’s Adjuvant-
induced Arthritis via their Anti-inflammatory and Gene Expression
of COX-1, IL-6 and IL-4
Authors: Ahmed Rania Hamed , Rashad  Ahmed Rasha , Galaly Sanaa Rida , Moustafa Nadia , Abourehab Mohammed Abdelwahab Sayed , Abdelgawad Mohamed Abdelwahab , Ahmed Osama Mohamed , Abdul-Hamid Manal 
Published: February 2023

Background and Objectives:
Rheumatoid arthritis (RA) is a type of arthritis that damages
joints and can affect the thymus and the spleen. RA is an autoimmune disorder in which the
immune system targets the body’s own tissues. The causes of RA are unknown, although a genetic
link is thought to be involved. The objective of this research was to evaluate the effect of
curcumin, mesenchymal stem cells (MSCs), and their combination on the disruption of serum cytokines,
ankle joint, thymus and spleen histopathology, and affected genes in complete Freund’s
adjuvant (CFA)-induced arthritis in male and female Wistar rats.

Methods:
Experimental animals were organized into 16 groups (6 animals for each), eight groups
including male rats and the other eight groups including females rats. The groups are normal control,
CMC, curcumin, MSCs, CFA, CFA/curcumin, CFA/ MSCs and the arthritic group treated
with MSCs and curcumin. One subcutaneous injection of 0.1 mL CFA was given to rats into the
right hind leg footpad to induce RA. The arthritic rats were intravenously injected three times
with bone marrow-derived MSCs (BM-MSCs) and/or treated orally with curcumin daily (100 mg
per kg body weight per day) for 21 days.

Results:
Curcumin and BM-MSCs work together to dramatically (P < 0.05) restore the high serum
PGE2 and IL-17 levels and lower the IL-13 level in arthritic rats to normal levels. Deleterious
effects on the spleen and thymus histological structure were counteracted. Gene expression
of COX-1 and IL-6 was increased and IL-4 was decreased; these changes were improved by the
combination treatment (P < 0.05).

Conclusion:
Based on these findings, additive therapeutic effects on RA occur from the combined
treatment of curcumin and BM-MSCs compared with their individual use (P < 0.05). Thus,
it can be said that both curcumin and BM-MSCs are effective at reducing inflammation while also
having beneficial effects on the ankle joint, thymus and spleen.
Novel Benzylamine Derivatives: Synthesis, Anti-Mycobacterium Tuberculosis Evaluation and Predicted ADMET Properties
Authors: Sedibana Mmaserole R. , Leboho Tlabo C. 
Published: February 2023

Background:
Tuberculosis (TB), a disease caused by the bacillus bacteria Mycobacterium tuberculosis is one of the major contributors of ill health in the world. TB is ranked in the top 10 causes of death globally and it is the leading killer associated with a single infectious agent. According to the World Health Organization (WHO), global number of deaths associated with TB have been slowly declining with 1.3 million in reported 2016 and 2017, and 1.2 million reported in 2018 and 2019.

Objective:
The synthesis, characterisation, biological evaluations, and the prediction of ADMET properties of the novel benzylamine derivatives.

Methods:
Commercially available reagents and solvents were purchased from Sigma Aldrich and Merck (South Africa). All chemicals were used as received, unless otherwise stated. The synthesised crude compounds were purified by flash silica gel column chromatography (5 – 30% ethyl acetate in hexane). The successful formation and purity of the synthesised compounds was confirmed by NMR, HRMS and melting point.

Results:
The respective organic compounds were synthesised by treating 3-ethoxysalcyladehyde, 5-bromo-3-ethoxysalcyladehyde, 5-chloro-3-ethoxysalcyladehyde with various aromatic amines and the products were obtained in good to excellent yields. The 1H and 13C NMR spectra of all the products showed the appearance of the methylene signals ranging from 3.88 – 4.68 ppm and 42.25 – 52.57 ppm respectively. Additionally, most compounds showed anti-Mycobacterium tuberculosis activity that ranged between 20 and 28 µM.

Conclusion:
A total of 36 compounds were synthesised and successfully biologically evaluated against Mycobacterium tuberculosis (Mtb) H37RV strain. All compounds showed activity against Mtb at concentrations of > 20 µM < 28 µM with the exception of compound one that was active against Mtb at higher concentration (MIC90 > 125 µM).
Volatile Organic Compounds in Patients’ Breath during Conversation
Authors: Ito Masaaki , Mimura Tatsuya , Noma Hidetaka , Inoue Yuji , Kawashima Makoto , Mizuno Yoshinobu , Watanabe Emiko , Mizota Atsushi 
Published: February 2023

Purpose:
The protracted coronavirus disease (COVID-19) pandemic has caused an unprecedented global health, social, economic, and psychological crisis. COVID-19 is transmitted via droplets, which include volatile organic compounds (VOCs) emitted by COVID-19 carriers. As a result, medical healthcare workers interacting with COVID-19 patients are at a high risk of infection. In this study, we measured the concentration of total VOCs (TVOCs) in the droplets of patients during conversations.

Methods:
Thirty patients aged 20–88 years were enrolled in this study. The amounts of VOCs, formaldehyde (HCHO), and carbon dioxide (CO2) as surrogate parameters for the patient’s droplets were measured at a distance of 1 m from the patients under the following conditions: 1) no conversation with a mask on, 2) conversation with a mask on, 3) conversation without a mask on, and 4) no conversation without a mask on.

Results:
The average concentrations of TVOCs (mg/m3), HCHO (mg/m3), and CO2 (ppm) were all the lowest before the masked conversation (1.79 ± 1.72, 0.25 ± 0.25, 1193 ± 516), increased during the masked conversation (1.99 ± 1.87, 0.29 ± 0.24, 1288 ± 555), were the highest during the unmasked conversation (3.10 ± 1.86, 0.45 ± 0.28, 1705 ± 729), and decreased to baseline after the unmasked conversation (1.89 ± 1.88, 0.26 ± 0.27, 1191 ± 518, respectively). Variations in TVOC and HCHO concentrations were positively correlated with patient age (TVOC: r = 0.42, p = 0.019 and HCHO: r = 0.47, p = 0.008).

Conclusion:
Wearing a mask reduced the VOC concentrations measured during conversations more than when a mask was not worn. Therefore, wearing a mask can reduce the emission of airborne droplet-derived VOCs and thereby reduce the risk of transmission of unknown patient-derived infections.

Clinical Trial Registration no:
The Clinical Trial Registration no: (UMIN000039595)