bluuebunny/crossref_metadata_2025_split huggingface dataset:
Species Diversity and Assemblage of Mangroves at Setiu Wetland, Terengganu, Malaysia
Authors: RAHMAN ABUL FAIZ MOHAMMAD ARIFUR , ISLAM MOHAMMAD AHSANUL , IDRIS MOHD HANAFI , BHUIYAN MD KHURSHID ALAM , CHOWDHURY MOHAMMAD MAMUN , ABUALREESH MUYASSAR H. , ABU HENA MUSTAFA KAMAL  
Published: June 2023

Mangroves in wetland ecosystems are diverse and play significant role in the adjacent communities on which they are dependent for their livelihoods. It is also important for fishery resources and nutrient inputs in marine and brackish water ecosystems. However, little is known about the tropical wetland lagoon ecosystems, particularly mangrove diversity and assemblages. Therefore, this present study was initiated to observe the mangrove species diversity and assemblages together with the conservation status in an important tropical wetland ecosystem in Setiu, Terengganu, Malaysia. In a variety of landward and small fringe island areas, three zones of square plots were selected (zones 1, 2 and 3) to address the objectives of this study. As a result, a total of 20 true mangrove species belonging to 11 genera from nine families were documented, of which, Avicennia rumphiana was listed as vulnerable (VU) by the IUCN. Twelve species of mangrove associates from 11 genera and nine families were also found in the investigated zones at Setiu. One of the mangrove associates, Intsia bijuga, was designated as vulnerable (VU) by the IUCN. The Shannon diversity index (H') of mangroves was found to be 1.08 at Setiu Wetland. Rhizophora mucronata was found to be well-expanded (H' = 1.05) followed by A. rumphiana, A. officinalis, Heritiera littoralis, A. corniculatum. Excoecaria agallocha, Lumnitzera racemosa, and A. ebracteatus (H' = 0.0) as the lowest. The findings of the present study revealed that mangroves in the Setiu Wetland are diverse and healthy compared to other mangrove ecosystems in the region. To maintain the health and function of the mangrove ecosystem in Setiu Wetland, proper monitoring is required.
PENGARUH PERSEKITARAN BAHASA TERHADAP PENGUASAAN KOSA KATA ARAB PELAJAR
Authors: Abdul Rahman Khairulaizam , Baharudin Harun 
Published: June 2023

Faktor persekitaran berupaya menatijahkan peningkatan pencapaian pelajar dalam memahami isi pembelajaran serta dapat meningkatkan tahap penguasaan kosa kata. Kajian ini bertujuan melihat pengaruh persekitaran bahasa terhadap penguasaan kosa kata Arab dalam kalangan pelajar Sekolah Agama Menengah (SAM) di Selangor. Kajian ini dikendalikan dalam bentuk kaedah tinjauan melibatkan 23 buah SAM dengan sampel seramai 335 orang daripada populasi seramai 2,653 orang pelajar. Analisis inferens digunakan untuk menganalisis data melibatkan ujian Korelasi Pearson dan Regresi Linear Berganda. Hasil dapatan menunjukkan bahawa persekitaran bahasa Arab (dimensi kesepaduan pelajar, penglibatan pelajar dan sikap pelajar berorientasikan tugasan) secara signifikannya memberi sumbangan terhadap penguasaan kosa kata Arab pelajar, dengan peratus varian sebanyak 31%. Dalam hal ini, dimensi kesepaduan pelajar merupakan penyumbang terbesar, iaitu sebanyak 8.57%. Kajian ini menunjukkan bahawa pembentukan ruang persekitaran pembelajaran perlu diperkasa melalui interaksi aktif sesama pelajar dan menambah baik sokongan guru supaya kestabilan ruang persekitaran pembelajaran bahasa dapat dibentuk.
Knowledge, Attitude and Self-efficacy Towards Blood Donation Among University Students: An Online Intervention Study
Authors: Md Mizanur Rahman  , JAMILAH BINTI HASIM  
Published: June 2023

Long-term shortages in the blood supply perpetuate increased morbidity and mortality from treatable diseases. However, how much people are motivated to donate blood was less studied, especially among university students. This study aimed to determine the effectiveness of online intervention for knowledge, attitude, and self-efficacy towards blood donation among undergraduate university students. A pre-and post-test study was conducted among undergraduate students. Four hundred students were invited to the study. Knowledge, positive and negative attitudes, and self-efficacy towards blood donation were measured using a structured questionnaire. Two digital infographics for blood donation and one 10-minute video were used as intervention materials. Following the intervention, statistical and practical significance was reported. Multivariate analysis was done to determine the differences in invention effect across age, gender, and study discipline. Following the intervention, 29.1% and 5.1% increased knowledge and self-efficacy from the base, respectively. The positive attitude towards blood donation increased by 2.8%, while the negative attitude decreased by 2.1%. Multivariate analysis of variance showed that online health education on blood donation is effective irrespective of age, gender, and discipline of study. Due to the Covid-19 pandemic, the intervention was integrated into a remote health intervention model based on the internet or mobile communication network. This study has provided a scientific conclusion on whether the intervention could motivate people to donate blood.
Motivasi dan Penggunaan Facebook, Instagram, Twitter dan TikTok dalam Kalangan Pelajar Universiti Malaysia Sarawak (UNIMAS)
Authors: Hussin Siti Haslina , Abu Bakar Nur Hazwani 
Published: June 2023

Kajian ini bertujuan untuk mengenalpasti motivasi dan penggunaan masa penggunaan Facebook, Instagram, Twitter dan TikTok dalam kalangan pelajar universiti di Malaysia. Data untuk kajian deskriptif diperoleh daripada 100 orang pelajar yang mengisi borang soalselidik secara atas talian. Dapatan kajian menunjukkan responden memilih Tiktok sebagai platform yang paling utama digunakan. Namun, Twitter adalah platform yang paling memenuhi motivasi pelajar menggunakan media sosial. Motivasi pelajar untuk menggunakan media sosial ialah dokumentasi kendiri dan ekspresi kendiri adalah tertinggi. Pengaruh daripada pertambahan jumlah pengikut mendorong pengguna untuk mendedahkan dan berkongsi tentang diri mereka kepada pengguna lain. Tidak ramai pelajar dimotivasi oleh konstruk hiburan dan kemudahan untuk menggunakan media sosial. Terdapat perbezaan ketara dalam penggunaan media sosial antara pelajar lelaki dan perempuan. Pelajar lelaki lebih gemar menggunakan Instagram berbanding pelajar perempuan yang lebih mengutamakan penggunaan Facebook dan Tiktok. Kebanyakan pelajar menggunakan media sosial melebihi lima jam sehari. Implikasi kajian ini menunjukkan bahawa ciri Twitter sebagai microblog boleh digunakan di dalam proses pembelajaran. Pertambahan responden dari pelbagai institusi dan pertambahan media sosial untuk dikaji dicadangkan untuk kajian masa akan datang.
Garden Plus +
Authors: Wong Jun Xiang  
Published: June 2023

Given how close the school is to the site, it gives one the idea of designing a playful space for the youth. With children as the target user, the modular structure needs to have multiple functions while bringing liveliness to the garden. Mini gardens promote gardening skills while the rock garden is enhanced. Music promotes interest and interactive plays. Picnics promote gatherings. The passage promotes views and perhaps photographic activities.
Lifetime breeding-site and nest-site fidelity in a declining terrestrial toadlet: evidence for a win-stay/lose-shift strategy
Authors: Byrne Phillip G. , Silla Aimee J. 
Published: June 2023

IntroductionBreeding-site fidelity can occur at various spatial scales and can vary in strength across these scales. Understanding this variation, and rules governing individual site-fidelity decisions, can have important implications for the conservation and management of threatened species. Globally, amphibians are in rapid decline and breeding-site fidelity appears to be widespread, yet few studies have investigated patterns of lifetime breeding-site fidelity, and no studies have explored decision-making rules in situ.MethodsWe investigated lifetime patterns of breeding site fidelity in the brown toadlet Pseudophryne bibronii, a species displaying population declines and local extinction throughout its range. We monitored a single population for a period spanning 10 years to establish the extent that males express site fidelity at the scale of the breeding site and the breeding patch. We also examined male nest-site fidelity between breeding years in relation to mating success to examine if toadlets follow a Win-stay/Lose shift strategy.ResultsOverall, we found that males displayed extreme lifetime fidelity to specific breeding patches within the breeding site, but that males regularly moved nest-site locations between breeding years. The degree of nest-site fidelity was related to male-mating success, whereby successful males established nests closer to a previous years’ nest location than unsuccessful males.DiscussionOur findings suggest that brown toadlets display extreme site fidelity at the scale of the breeding patch, but that within patches male nesting decisions are flexible and follow a win-stay/lose-shift strategy. These results provide novel evidence that breeding site fidelity in amphibians can vary depending on spatial scale (indicative of scale-dependent information use) and that a rule-based learning strategy can influence the degree of nest-site fidelity. Breeding patch fidelity and capacity for spatial learning may be widespread in long-lived amphibians and necessitate in situ conservation strategies that protect known breeding patches (and adjacent habitat) whilst enabling unconstrained localised movement.
Timing of heart failure development and clinical outcomes in patients with acute myocardial infarction
Authors: Kim Hyung Yoon , Kim Kye Hun , Lee Nuri , Park Hyukjin , Cho Jae Yeong , Yoon Hyun Ju , Ahn Youngkeun , Jeong Myung Ho , Cho Jeong Gwan 
Published: June 2023

Background and objectivesTo investigate the clinical relevance of the timing of heart failure (HF) development on long-term outcome in patients with acute myocardial infarction (AMI).Materials and methodsA total of 1,925 consecutive AMI patients were divided into 4 groups according to the timing of HF development; HF at admission (group I, n = 627), de novo HF during hospitalization (group II, n = 162), de novo HF after discharge (group III, n = 98), no HF (group IV, n = 1,038). Major adverse cardiac events (MACE) defined as the development of death, re-hospitalization, recurrent MI or revascularization were evaluated.ResultsHF was developed in 887 patients (46.1%) after an index AMI. HF was most common at the time of admission for AMI, but the development of de novo HF during hospitalization or after discharge was not uncommon. MACE was developed in 619 out of 1,925 AMI patients (31.7%). MACE was highest in group I, lowest in group IV, and significantly different among groups; 275 out of 627 patients (43.9%) in group I, 64 out of 192 patients (39.5%) in group II, 36 out of 98 patients (36.7%) in group III, and 235 out of 1,038 patients (22.6%) in group IV (P &lt; 0.001). MACE free survival rates at 3 years were 56% in group I, 62% in group II, 64% in group III, and 77% in group IV (P &lt; 0.001).ConclusionsHF was not uncommon and can develop at any time after an index AMI, and the development of HF was associated with poor prognosis. The earlier the HF has occurred after AMI, the poorer the clinical outcome was. To initiate the guideline directed optimal medical therapy, therefore, the development of HF should be carefully monitored even after the discharge from an index AMI.
Exploration of the association between the single-nucleotide polymorphism of co-stimulatory system and rheumatoid arthritis
Authors: Chen Ding-Ping , Wen Ying-Hao , Lin Wei-Tzu , Hsu Fang-Ping , Yu Kuang-Hui 
Published: June 2023

IntroductionThe human leukocyte antigen (HLA) has been linked to the majority of autoimmune diseases (ADs). However, non-HLA genes may be risk factors for ADs. A number of genes encoding proteins involved in regulating T-cell and B-cell function have been identified as rheumatoid arthritis (RA) susceptibility genes.MethodsIn this study, we investigated the association between RA and single-nucleotide polymorphisms (SNPs) of co-stimulatory or co-inhibitory molecules in 124 RA cases and 100 healthy controls without immune-related diseases [including tumor necrosis factor superfamily member 4 (TNFSF4), CD28, cytotoxic T-lymphocyte–associated protein 4 (CTLA4), and programmed cell death protein 1 (PDCD1)].ResultsThe results showed that there were 13 SNPs associated with RA, including rs181758110 of TNFSF4 (CC vs. CT, p = 0.038); rs3181096 of CD28 (TT vs. CC + CT, p = 0.035; CC vs. TT, p = 0.047); rs11571315 (TT vs. CT, p = 0.045), rs733618 (CC vs. TT + CT, p = 0.043), rs4553808 (AA vs. AG vs. GG, p = 0.035), rs11571316 (GG vs. AG vs. AA, p = 0.048; GG vs. AG + AA, p = 0.026; GG vs. AG, p = 0.014), rs16840252 (CC vs. CT vs. TT, p = 0.007; CC vs. CT, p = 0.011), rs5742909 (CC vs. CT vs. TT, p = 0.040), and rs11571319 of CTLA4 (GG vs. AG vs. AA, p &lt; 0.001; GG vs. AG + AA, p = 0.048; AA vs. GG + AG, p = 0.001; GG vs. AA, p = 0.008; GG vs. AG, p ≤ 0.001); and rs10204525 (TT vs. CT + CC, p = 0.024; TT vs. CT, p = 0.021), rs2227982 (AA vs. GG, p = 0.047), rs36084323 (TT vs. CT vs. CC, p = 0.022; TT vs. CT + CC, p = 0.013; CC vs. TT + CT, p = 0.048; TT vs. CC, p = 0.008), and rs5839828 of PDCD1 (DEL vs. DEL/G vs. GG, p = 0.014; DEL vs. DEL/G + GG, p = 0.014; GG vs. DEL + DEL/G, p = 0.025; DEL vs. GG, p = 0.007).DiscussionConsequently, these SNPs may play an important role in immune regulation, and further research into the role of these SNPs of immune regulatory genes in the pathogenesis of RA is required.
Characterizing distinct profiles of immune and inflammatory response with age to Omicron infection
Authors: Zhang Lina , Wang Zhanwen , Lyu Feng , Liu Chun , Li Chunhui , Liu Wei , Ma Xinhua , Zhou Jieyu , Qian Xinyu , Qian Zhaoxin , Lu Yong 
Published: June 2023

BackgroundUnderstanding inflammatory and immune responses to Omicron infection based on age is crucial when addressing this global health threat. However, the lacking of comprehensive elucidation hinders the development of distinct treatments tailored to different age populations.Methods1299 cases of Omicron infection in Shanghai were enrolled between April 10, 2022 and June 3, 2022, dividing into three groups by ages: Adult group (18-59 years), Old group (60-79 years), and Elder group (≥ 80 years). Laboratory data including inflammatory cytokines, cellular, and humoral immunity were collected and analyzed.ResultsThe mean age of Adult, Old, and Elder groups were 44.14, 69.98, and 89.35 years, respectively, with 40.9% being men. The Elder group patients exhibited higher white blood cell (WBC) counts and elevated levels of inflammatory cytokines, but their lymphocyte counts were relatively lower. In comparison to the Old group patients, the Elder group patients demonstrated significantly lower CD3+ T-cell counts, CD3+ T-cell proportion, CD4+ T-cell counts, CD8+ T-cell counts, and CD19+ B-cell counts, while the NK-cell counts were higher. Omicron negative patients displayed a higher proportion of CD19+ B-cells and higher levels of Complement-3 and IL-17 compared to the positive patients in the Old group. Omicron negative patients had lower WBC counts, CD3+CD8+ T-cells proportion, and the levels of serum amyloid A and IgA in the Elder group, but the CD4+/CD8+ ratio was higher.ConclusionsOur study identified the distinct profiles of inflammatory and immune responses to Omicron infection varying with age and highlighted the diverse correlations between the levels of various biomarkers and Omicron infected/convalescent patients.
Downregulation of monocyte miRNAs: implications for immune dysfunction and disease severity in drug-resistant tuberculosis
Authors: Sampath Pavithra , Moorthy Manju , Menon Athul , Madhav Lekshmi , Janaki Aishwarya , Dhanapal Madhavan , Natarajan Alangudi Palaniappan , Hissar Syed , Ranganathan Uma Devi , Ramaswamy Gopalakrishna , Bethunaickan Ramalingam 
Published: June 2023

BackgroundMonocyte miRNAs govern both protective and pathological responses during tuberculosis (TB) through their differential expression and emerged as potent targets for biomarker discovery and host-directed therapeutics. Thus, this study examined the miRNA profile of sorted monocytes across the TB disease spectrum [drug-resistant TB (DR-TB), drug-sensitive TB (DS-TB), and latent TB] and in healthy individuals (HC) to understand the underlying pathophysiology and their regulatory mechanism.MethodsWe sorted total monocytes including three subsets (HLA-DR+CD14+, HLA-DR+CD14+CD16+, and HLA-DR+CD16+cells) from peripheral blood mononuclear cells (PBMCs) of healthy and TB-infected individuals through flow cytometry and subjected them to NanoString-based miRNA profiling.ResultsThe outcome was the differential expression of 107 miRNAs particularly the downregulation of miRNAs in the active TB groups (both drug-resistant and drug-sensitive). The miRNA profile revealed differential expression signatures: i) decline of miR-548m in DR-TB alone, ii) decline of miR-486-3p in active TB but significant elevation only in LTB iii) elevation of miR-132-3p only in active TB (DR-TB and DS-TB) and iv) elevation of miR-150-5p in DR-TB alone. The directionality of functions mediated by monocyte miRNAs from Gene Set Enrichment Analysis (GSEA) facilitated two phenomenal findings: i) a bidirectional response between active disease (activation profile in DR-TB and DS-TB compared to LTB and HC) and latent infection (suppression profile in LTB vs HC) and ii) hyper immune activation in the DR-TB group compared to DS-TB.ConclusionThus, monocyte miRNA signatures provide pathological clues for altered monocyte function, drug resistance, and disease severity. Further studies on monocyte miRNAs may shed light on the immune regulatory mechanism for tuberculosis.
Protocol for the development of a reporting guideline for clinical trials with integrated Chinese and western medicine interventions: the CONSORT extension for ICWM
Authors: Wang Juan , Zhang Xuan , Wang Ping , Han Fei , Li Ji , Ma Yanfang , Lyu Aiping , Bian Zhaoxiang 
Published: June 2023

BackgroundWhile Integrated Chinese and Western Medicine (ICWM) has become widely accepted as a necessary intervention for treating various diseases, key information about ICWM interventions is often missing in published clinical trials. To facilitate complete, transparent, and consistent reporting of clinical trials with ICWM interventions, an extension of the CONSORT guideline is necessary to be developed: the CONSORT-ICWM guideline.MethodsThe CONSORT-ICWM guideline will be developed in five stages in accordance with recommendations for the development of reporting guidelines from the EQUATOR (Enhancing the QUAlity and Transparency Of health Research) Network, including (1) project launch and registration; (2) literature review and checklist draft; (3) Delphi survey; (4) consensus meeting; and (5) finalization of the guideline. Additionally, the working group will be composed of professors with expertise in integrated medicines, traditional Chinese medicines, biomedical informatics, statistics, methodology, development of reporting guidelines, epidemiology, health economics, and paper publications.DiscussionThe CONSORT-ICWM guideline is to improve the reporting quality of clinical trials with ICWM interventions by ensuring the reports are complete, informative, clear, and transparent.
Kunxian capsule alleviates podocyte injury and proteinuria by inactivating β-catenin in db/db mice
Authors: Jin Bo , Liu Jing , Zhu Yan , Lu Jian , Zhang Qingyan , Liang Yan , Shao Qiuyuan , Jiang Chunming 
Published: June 2023

BackgroundDiabetic kidney disease (DKD) remains the primary cause of end-stage renal disease (ESRD) globally, but treatment options are limited. Kunxian capsule (KXC) has been utilized for the treatment of autoimmune diseases and IgA nephropathy in China. However, its effect on DKD remains poorly investigated. Therefore, this study aimed to explore the protective effect of KXC in db/db mice and elucidate its underlying mechanism.MethodsThe renoprotective effects of KXC were assessed in a DKD mouse model using male BKS db/db diabetic mice. After 8 weeks of treatment, the urinary albumin-to-creatinine ratio (UACR), blood biochemical parameters, renal histopathological manifestation, and podocyte ultrastructural changes were evaluated. Additionally, the expression of podocyte epithelial-to-mesenchymal transition (EMT) markers [WT1, ZO-1, and collogen I (Col1a1)] was quantitatively analyzed. Furthermore, we explored the role of KXC in the β-catenin signaling pathway to elucidate the underlying mechanism of KXC’s renoprotective effect.ResultsKXC treatment effectively reduced albuminuria and attenuated renal structural abnormalities in db/db mice. Additionally, KXC restored the protein and mRNA expression of WT1 and ZO-1 while suppressing the expression of Col1a1 in db/db mice, indicating its ability to alleviate podocyte EMT. Mechanistically, KXC exerted a significant suppressive effect on the activation of β-catenin signaling in diabetic kidneys.ConclusionKXC has the potential to protect podocytes during DKD by alleviating podocyte EMT through inactivating β-catenin signaling.