bluuebunny/crossref_metadata_2025_split huggingface dataset:
miRNA-576-5p promotes endometrial cancer cell growth and metastasis by targeting ZBTB4
Authors: Chen Chen , Zhang Qing , Kong Beihua 
Published: December 2022

Abstract
Purpose
MicroRNAs (miRNAs) have already been shown to have a strong correlation with the invasion and metastasis capacity of tumor cells. The present research examined the function of miRNA-576-5p (miR-576-5p) in the development of endometrial cancer (EC).

Methods
miR-576-5p and ZBTB4 expression in EC and benign endometrial tissues was measured using quantitative real-time PCR (qRT-PCR) and western blot. To evaluate the proliferation ability of tumor cells in vitro, 2,5-diphenyl-2H-tetrazolium bromide (MTT) and colony formation assays were carried out. The effect of miR-576-5p on the proliferation ability of EC cells in vivo was measured by the tumor formation in nude mice. The migration and invasion ability of tumor cells was determined using the transwell assay. To confirm the association between expressions of miR-576-5p and zinc finger and BTB domain containing four (ZBTB4), western blot, qRT-PCR, and luciferase assay were carried out.

Results
miR-576-5p expression increased significantly in EC samples than in benign endometrial tissues. The level of miR-576-5p was significantly higher in the polymerase ε (POLE) ultramutated subgroup compared to the other three subgroups. High levels of miR-576-5p expression were linked to a shorter progression-free interval time in the copy number high subgroup. Furthermore, upregulated miR-576-5p facilitated EC cell invasion and migration in vitro and promoted the proliferation of EC tumor cell lines both in vitro and in vivo. Moreover, this study showed that the expression of ZBTB4 decreased in patients with EC, and the dual-luciferase reporter assay confirmed that miR-576-5p binds directly to the 3′-UTR of ZBTB4 and inhibits the expression of ZBTB4. An increase in miR-576-5p expression leads to a decrease in the mRNA and protein expression level of ZBTB4. The effects of miR-576-5p can be reversed by overexpression of ZBTB4.

Conclusion
miR-576-5p promoted proliferation and metastasis capacity of EC cells by inhibiting ZBTB4 expression. We hypothesized that miR-576-5p could be a prospective therapeutic target for EC.
L'Ancien Régime Legacy of the Chinese Governance: Grand Union and the Common Good
Authors: Zhang Yan 
Published: December 2022

AbstractGovernance is essentially a dynamic mechanism to promote collective action for the common good. The Chinese concept of the public/common (gōnggòng) and its political philosophy of Grand Union governance provide an alternative perspective for understanding such dynamics from the unit of family through institutions to the state-embedded society. Three critical arguments with interconnected elements are analysed: governance as a dynamic mechanism; review of the historical, philosophical and political legacy of Chinese governance; and proposal of a framework for governance of the Grand Union. It concludes that ‘Chinese characteristics’, such as diverse adaptations, gradual flexibility, experimental pragmatism, and polycentric balance with a strong central state are all deeply rooted in its pre-revolutionary ancien régime. In order to understand these seemingly different institutions and values, we need to revisit their original functionality of governing for the common good.
Thalamocortical Circuit Controls Neuropathic Pain via Up-regulation of HCN2 in the Ventral Posterolateral Thalamus
Authors: Yan Yi , Zhu Mengye , Cao Xuezhong , Xu Gang , Shen Wei , Li Fan , Zhang Jinjin , Luo Lingyun , Zhang Xuexue , Zhang Daying , Liu Tao 
Published: December 2022

AbstractThe thalamocortical (TC) circuit is closely associated with pain processing. The hyperpolarization-activated cyclic nucleotide-gated (HCN) 2 channel is predominantly expressed in the ventral posterolateral thalamus (VPL) that has been shown to mediate neuropathic pain. However, the role of VPL HCN2 in modulating TC circuit activity is largely unknown. Here, by using optogenetics, neuronal tracing, electrophysiological recordings, and virus knockdown strategies, we showed that the activation of VPL TC neurons potentiates excitatory synaptic transmission to the hindlimb region of the primary somatosensory cortex (S1HL) as well as mechanical hypersensitivity following spared nerve injury (SNI)-induced neuropathic pain in mice. Either pharmacological blockade or virus knockdown of HCN2 (shRNA-Hcn2) in the VPL was sufficient to alleviate SNI-induced hyperalgesia. Moreover, shRNA-Hcn2 decreased the excitability of TC neurons and synaptic transmission of the VPL–S1HL circuit. Together, our studies provide a novel mechanism by which HCN2 enhances the excitability of the TC circuit to facilitate neuropathic pain.
And the Flesh in Between: Towards a Health Semiotics
Authors: Schiller Devon 
Published: December 2022

AbstractThe call for a biosemiotic perspective within medical semiotics has been steadily increasing over the late twentieth and early twenty-first centuries. In Food and Medicine: A Biosemiotic Perspective, Yogi Hale Hendlin, Johnathan Hope, and the nine contributions in their edited volume boldly seek to bridge the segregation between nature and culture in the medical sciences as well as in the medical humanities. To a large extent, they achieve this aim by explicating the sign relations in food and medicine, the sign relations of medical theory and practice, and the sign relations between the biology in medicine and medicine of society. Taking up a semio-historical approach, I contextualize two select contributions from Hendlin and Hope’s Food and Medicine with the medical semiotics of the Hippocratic tradition. By comparing the biological semiotics from the contributions to the medical semiotics from the Corpus, I critically explicate the ways in which biosemiotics moves this subdiscipline forward and why the perspective is significant not only for the health of humans, but also for the health of other animals, and indeed for the health of the planet that we all inhabit together. On these grounds, I propose a turn from medical semiotics to health semiotics. This program for semiotics would encompass not only food and medicine, but also lifestyle and wellbeing, as well as the subjective, qualitative perspectivism that makes biosemiotics frontier research, thereby constituting a biosemioethics and promoting a semiotic fitness.
The visibility of the human eye in blowing snow with the effect of suspended snow particles’ afterimages
Authors: Sakurai Toshimitsu , Takechi Hirotaka , Kokubu Tetsuya , Matsuzawa Masaru 
Published: December 2022

AbstractAfterimages of snow particles in blowing snow may cause lower visibility of the human eye compared to images captured by a digital video camera. However, the impact of the afterimage of snow particles on the visibility of the human eye in blowing snow has not been investigated quantitatively. Here we examined visibility taking the effect of afterimages of snow particles on the human eye into consideration. By considering the duration range of afterimages, and changing the windspeed while the snow density remains constant, our numerical model of visibility for the visual duration range of 0.12 to 0.04 s could accurately explain the visibility of the human eye previously observed in blowing snow.
Medical Care Among Individuals with a Concussion in Ontario: A Population-based Study
Authors: Langer Laura Kathleen , Bayley Mark Thedore , Levy Charissa , Munce Sarah Elizabeth Patricia , Lawrence David Wyndham , Tam Alan , de Oliveira Claire 
Published: December 2022

ABSTRACT:Background:Concussion affects 1.2% of the population annually; rural regions and children have higher rates of concussion.Methods:Using administrative health care linked databases, all residents of Ontario with a physician diagnosed concussion were identified using ICD-9 code 850 or ICD-10 code S06. Cases were tracked for 2 years for concussion-related health care utilization with relevant specialist physicians (i.e., neurology, otolaryngology, physiatry, psychiatry, ophthalmology). Billing codes, specialist codes, and time from index to visit were analyzed. Factors associated with increased specialist visits were also examined.Results:In total, 1,022,588 cases were identified between 2008 and 2014 with 2 years of post-concussion health care utilization available. Follow-up by physician within 3 days of injury occurred in only 14% of cases. Mean time between ED diagnosis and follow-up by a physician was 83.9 days, whereas for rural regions it was >100 days. About half of adults (51.9%) and children (50.3%) had at least 1 specialist visit following concussion. Mean time between injury and first specialist visit was 203.8 (SD 192.9) days for adults, 213.5 (SD 201.0) days for rural adults, and 276.0 (SD 202.6) days for children. There were 67,420 neurology visits, 70,404 psychiatry visits, 13,571 neurosurgery visits, 19,780 physiatry visits, 101,788 ENT visits, and 103,417 ophthalmology visits in the 2 years tracking period. Factors associated with more specialist use included age > 18 years, urban residence, and pre-injury psychiatric history.Conclusions:There are discrepancies in post-concussion health care utilization based on age group and rural/urban residence. Addressing these risk factors could improve concussion care access.
Two masks can be worse than one: N95 respirator failure caused by an overlying face mask
Authors: Mueller Jeffrey T. , Geyer Holly D. , Karimi Soroor , Poterack Karl A. , Seville Maria Teresa A. , Buckner Petty Skye , Tipton Steven M. 
Published: December 2022

AbstractWe have demonstrated the effect of covering an N95 filtering facepiece respirator (FFR) with an overlying face mask. In total, 100 participants successfully completed quantitative fit testing wearing a 3M 1870+ FFR. Among them, 13 (13%; 95% CI, 7%–22%) failed subsequent fit testing when simultaneously wearing a Halyard 47117 procedural mask over the FFR.
Response of black swallowwort (Vincetoxicum nigrum) to herbicides plus mowing
Authors: Milbrath Lindsey R. , Biazzo Jeromy , Morris Scott H. , DiTommaso Antonio 
Published: December 2022

AbstractThe invasive vine black swallowwort [Vincetoxicum nigrum (L.) Moench = Cynanchum louiseae Kartesz & Gandhi, Apocynaceae] is difficult to control, and herbicide studies are lacking. This long-lived perennial species is primarily found in high-light environments in natural areas and perennial cropping systems in northeastern North America. We conducted a 3-yr herbicide efficacy study, with or without mowing, in an old-field site infested with V. nigrum in Dutchess County, NY, USA. Experimental plots were either herbicide treated in early July or mowed in early July and subsequently herbicide treated in late August for 2 yr with the potassium salt of glyphosate (2.02 kg ae ha−1), the isopropylamine salt of glyphosate (1.35 kg ae ha−1), or the butoxyethyl ester of triclopyr (1.79 kg ae ha−1). Both glyphosate formulations were effective in reducing V. nigrum aboveground biomass, although they were somewhat less effective in reducing cover or stem densities of V. nigrum plants >10-cm tall after 2 yr compared with untreated plots. Mowing did not always enhance the efficacy of foliar glyphosate applications. Triclopyr, with or without mowing, was generally not effective against V. nigrum in our study. The only significant effect of triclopyr was to increase the cover of grasses in the plots. While annual applications of glyphosate can be useful for management of V. nigrum infestations, higher rates and more frequent applications of triclopyr need to be investigated to determine its usefulness for V. nigrum control.
Latest Maastrichtian middle- and high-latitude mosasaurs and fish isotopic composition: carbon source, thermoregulation strategy, and thermal latitudinal gradient
Authors: Leuzinger Léa , Kocsis László , Luz Zoneibe , Vennemann Torsten , Ulyanov Alexey , Fernández Marta 
Published: December 2022

AbstractHere we report high-latitude stable isotope compositions of Maastrichtian fossil fish and marine reptiles (mainly mosasaurs) from Antarctica (64°S paleolatitude) and compare them with mid-paleolatitude samples from Argentine Patagonia (45°S). Disparities between the δ13C values of bony fish and marine reptiles correspond to differences in the foraging ground (distance from the shore and depth), while dramatically higher δ13C values (by 18‰) in shark enameloid cannot be explained through ecology and are here imputed to biomineralization. Comparison with extant vertebrates suggests that the diet alone can explain the offset observed between bony fish and mosasaurs; however, breath holding due to a diving behavior in mosasaurs may have had some impact on their δ13C values, as previously suggested. The δ18OPO4 values of the remains confirm a relatively stable, elevated body temperature for marine reptiles, meaning that they were thermoregulators. We calculated a water temperature of ~8°C for Antarctica from the fish δ18OPO4 values, warmer than present-day temperatures and consistent with the absence of polar ice sheets during the latest Maastrichtian. Our fish data greatly extend the latitudinal range of Late Cretaceous fish δ18OPO4 values and result in a thermal gradient of 0.4°C/1° of latitude when combined with literature data.
The Drafting of the Constitution of the Union of Burma in 1947: Dominion Status, Indo–Burmese Relations, and the Irish Example
Authors: Coffey Donal K. 
Published: December 2022

AbstractThis paper aims to consider four elements of Burmese constitutional history between 1946 and 1948. The first section considers the negotiations between Burma and the United Kingdom and argues that the debate about whether Burma wanted Dominion status has overlooked the crucial transitional government period. The second section gives a brief overview of the drafting process, paying particular attention to the links between the Indian Constituent Assembly in Delhi and Rangoon. The third section outlines the comparative influences on the Burmese Constitution using the digital humanities and illustrates that the biggest foreign influence was the Irish Constitution of 1937. The final section looks at the Burmese political leadership after World War II along two dimensions—at a nationalist level and at an elite level—and traces some of the links between Burma and Ireland.
Retrieval of a Real‐Time Sea Surface Vector Field From SAR Doppler Centroid: 2. On the Radial Velocity
Authors: Yang Xiaobo , He Yijun 
Published: December 2022

AbstractThe concept of radial velocity is proposed for high frequency (HF) radar for sea surface current measurement. However, in a spaceborne synthetic aperture radar (SAR) system, the meaning of this concept has changed greatly. Through the evidence and analysis presented in this paper, the radial velocity observed via spaceborne SAR is revealed from the projection of the satellite's velocity at different points along the range direction, and its distribution on the image presents a strip‐like texture, which is very different from the central radial distribution on the velocity image measured via HF radar. The retrieved sea surface velocity field does not exhibit any radial characteristics, and its value is solely the magnitude of the sea surface velocity.
Constraints on Moon's Orbit 3.2 Billion Years Ago From Tidal Bundle Data
Authors: Eulenfeld Tom , Heubeck Christoph 
Published: December 2022

AbstractThe angular momentum of the Earth‐Moon system was initially dominated by Earth's rotation with a short solar day of around 5 hr duration. Since then, Earth gradually transferred angular momentum through tidal friction to the orbit of the Moon, resulting in an increasing orbital radius and a deceleration of Earth's rotation. Geologic observations of tidal deposits can be used to verify and constrain models of lunar orbital evolution. In this work we reexamine the oldest tidal record suitable for analysis from the Moodies Group, South Africa, with an age of 3.22 billion years. Time frequency analysis of the series of thicknesses of the sandstone‐shale layers yields a periodicity of 15.0 layers, taking into account the possibility of missing laminae. Assuming a mixed tidal system, the duration of two neap‐spring‐neap cycles was 30.0 lunar days for dominant semidiurnal or 30.0 sidereal days for dominant diurnal tides. We derive the relationship between this observation and the past Earth‐Moon distance and re‐visit related published work. We find that the Earth‐Moon distance 3.2 billion years ago was about 70% of today's value. The Archean solar day was around 13 hr long. The ratio of solar to lunar tide‐raising torque controls the leakage of angular momentum from the Earth‐Moon system, but deviation from the assumed ratio of 0.211 results in only moderate changes. A duration of a postulated 21‐hr atmospheric resonance shorter than 200 million years would be consistent with our observation; it would significantly alter the Earth‐Moon distance.
Partitioned Soil Water Balance and Its Link With Water Uptake Strategy Under Apple Trees in the Loess‐Covered Region
Authors: Shi Peijun , Gai Haoqi , Li Zhi 
Published: December 2022

AbstractIt is important, but challenging, to partition soil water balance (SWB) to understand the impacts of afforestation on soil hydrological processes. This study will investigate the partitioning of SWB by combining stable and radioactive water isotopes to quantify the effects of afforestation, and analyze the mechanism by which SWB changes through identifying the water uptake strategies of apple trees of 18 and 26 years old (A18 and A26). Compared to the reference farmland, apple orchards significantly increased evapotranspiration (ET) by 5%–10%, which in turn decreased soil water storage and deep drainage by 5%–14% and 50%–95%, respectively. Further, the partitioning of ET showed that apple tree planting increased transpiration by 15%–28% but decreased evaporation by 17%–30%. The above change in SWB appeared to be closely related to plant water uptake strategies. The apple trees shifted their water source from shallow (0–2 m) to deep soils (below 2 m), utilizing approximately 62% of deep soil water in the late growing season. In particular, 23% of source water may come from soil water older than 50 years. The older apple trees tended to extract more water from deeper soils (45% for A26 vs. 38% for A18). Therefore, the soil water deficit was the cumulative effects of root water uptake. The methods for SWB partitioning provides technical support for similar studies, and the findings are helpful to better understand the hydrological processes in the thick loess deposition.
Plant microbiomes harbor potential to promote nutrient turnover in impoverished substrates of a Brazilian biodiversity hotspot
Authors: Camargo Antonio P , de Souza Rafael S C , Jose Juliana , Gerhardt Isabel R , Dante Ricardo A , Mukherjee Supratim , Huntemann Marcel , Kyrpides Nikos C , Carazzolle Marcelo F , Arruda Paulo 
Published: December 2022

Abstract
The substrates of the Brazilian campos rupestres, a grassland ecosystem, have extremely low concentrations of phosphorus and nitrogen, imposing restrictions to plant growth. Despite that, this ecosystem harbors almost 15% of the Brazilian plant diversity, raising the question of how plants acquire nutrients in such a harsh environment. Here, we set out to uncover the taxonomic profile, the compositional and functional differences and similarities, and the nutrient turnover potential of microbial communities associated with two plant species of the campos rupestres-dominant family Velloziaceae that grow over distinct substrates (soil and rock). Using amplicon sequencing data, we show that, despite the pronounced composition differentiation, the plant-associated soil and rock communities share a core of highly efficient colonizers that tend to be highly abundant and is enriched in 21 bacterial families. Functional investigation of metagenomes and 522 metagenome-assembled genomes revealed that the microorganisms found associated to plant roots are enriched in genes involved in organic compound intake, and phosphorus and nitrogen turnover. We show that potential for phosphorus transport, mineralization, and solubilization are mostly found within bacterial families of the shared microbiome, such as Xanthobacteraceae and Bryobacteraceae. We also detected the full repertoire of nitrogen cycle-related genes and discovered a lineage of Isosphaeraceae that acquired nitrogen-fixing potential via horizontal gene transfer and might be also involved in nitrification via a metabolic handoff association with Binataceae. We highlight that plant-associated microbial populations in the campos rupestres harbor a genetic repertoire with potential to increase nutrient availability and that the microbiomes of biodiversity hotspots can reveal novel mechanisms of nutrient turnover.
Restoring mitochondrial cardiolipin homeostasis reduces cell death and promotes recovery after spinal cord injury
Authors: Liu Nai-Kui , Deng Ling-Xiao , Wang Miao , Lu Qing-Bo , Wang Chunyan , Wu Xiangbing , Wu Wei , Wang Ying , Qu Wenrui , Han Qi , Xia Yongzhi , Ravenscraft Baylen , Li Jin-Lian , You Si-Wei , Wipf Peter , Han Xianlin , Xu Xiao-Ming 
Published: December 2022

AbstractAlterations in phospholipids have long been associated with spinal cord injury (SCI). However, their specific roles and signaling cascades in mediating cell death and tissue repair remain unclear. Here we investigated whether alterations of cardiolipin (CL), a family of mitochondrion-specific phospholipids, play a crucial role in mitochondrial dysfunction and neuronal death following SCI. Lipidomic analysis was used to determine the profile of CL alteration in the adult rat spinal cord following a moderate contusive SCI at the 10th thoracic (T10) level. Cellular, molecular, and genetic assessments were performed to determine whether CL alterations mediate mitochondrial dysfunction and neuronal death after SCI, and, if so, whether reversing CL alteration leads to neuroprotection after SCI. Using lipidomic analysis, we uncovered CL alterations at an early stage of SCI. Over 50 distinct CL species were identified, of which 50% showed significantly decreased abundance after SCI. The decreased CL species contained mainly polyunsaturated fatty acids that are highly susceptible to peroxidation. In parallel, 4-HNE, a lipid peroxidation marker, significantly increased after SCI. We found that mitochondrial oxidative stress not only induced CL oxidation, but also resulted in CL loss by activating cPLA2 to hydrolyze CL. CL alterations induced mitochondrial dysfunction and neuronal death. Remarkably, pharmacologic inhibition of CL alterations with XJB-5-131, a novel mitochondria-targeted electron and reactive oxygen species scavenger, reduced cell death, tissue damage and ameliorated motor deficits after SCI in adult rats. These findings suggest that CL alteration could be a novel mechanism that mediates injury-induced neuronal death, and a potential therapeutic target for ameliorating secondary SCI.
Hippocampus as a sorter and reverberatory integrator of sensory inputs
Authors: Nomoto Masanori , Murayama Emi , Ohno Shuntaro , Okubo-Suzuki Reiko , Muramatsu Shin-ichi , Inokuchi Kaoru 
Published: December 2022

AbstractThe hippocampus must be capable of sorting and integrating multiple sensory inputs separately but simultaneously. However, it remains to be elucidated how the hippocampus executes these processes simultaneously during learning. Here we found that synchrony between conditioned stimulus (CS)-, unconditioned stimulus (US)- and future retrieval-responsible cells occurs in the CA1 during the reverberatory phase that emerges after sensory inputs have ceased, but not during CS and US inputs. Mutant mice lacking N-methyl-D-aspartate receptors (NRs) in CA3 showed a cued-fear memory impairment and a decrease in synchronized reverberatory activities between CS- and US-responsive CA1 cells. Optogenetic CA3 silencing at the reverberatory phase during learning impaired cued-fear memory. Thus, the hippocampus uses reverberatory activity to link CS and US inputs, and avoid crosstalk during sensory inputs.