bluuebunny/crossref_metadata_2025_split huggingface dataset:
Genic-SSR-based genetic diversity and population structure analysis in a global germplasm collection highlights the African origin of winged bean (Psophocarpus tetragonolobus L.)
Authors: Tribhuvan Kishor U. , Mishra Twinkle , Dilip Samptirao , Panday Avinash , Kumar Sudhir , Mahto Jay Lal , Raigar Hanuman Lal , Pattanayak A. , Singh Binay Kumar 
Published: March 2023

Abstract

Winged bean (Psophocarpus tetragonolobus L.) is an underutilized legume of the family Fabaceae. We assembled 58,811 unigenes from the publicly available winged bean RNAseq data sets and discovered 4,107 perfect SSRs. Seventy-eight of the 166 SSRs amplified into a single band, of which 22 were polymorphic, in 79 germplasm accessions of winged bean constituting eight populations from India, Thailand, Nigeria, Indonesia, Taiwan, the Philippines, and Papua New Guinea. We found 60 alleles at the 22 polymorphic SSR loci, with a mean value of 2.73 per locus. With a mean of 0.36, the PIC values for the SSR loci ranged from 0.11 to 0.64. We recorded the maximum genetic diversity in advanced breeding lines (I = 0.67, He = 0.41) and the minimum genetic diversity in the germplasm accessions collected from Manipur (I = 0.44, He = 0.28), a north-eastern Indian state. The AMOVA analysis indicated that within-population variation was significantly higher (85%) than between-population variation (15%). The pairwise Nm values between the populations ranged between 0.69–3.41, indicating the varying level of gene flow between them. The analysis of the population structure based on the Bayesian model-based clustering algorithm revealed two distinct groups among the eight populations with different levels of introgression. The fuzzy clustering based on the Manhattan method also identified a similar number of groups, with 72% similarity between the two clustering methods. However, the Neighbour-Joining (NJ)-based clustering grouped all the accessions into four clusters. Nevertheless, all three clustering methods unanimously indicated that most African accessions tended to group, and their leftover members were spread across the hypothetical geographical populations, indirectly supporting the African origin of the winged bean.
Joining of cemented carbides WC-Co and tool steel X153CrMoV12 with capacitor discharge welding process
Authors: Stocks Nicolas , Kolarikova Marie , Chotěborský Rostislav , Vronka Marek , Kolařík Ladislav 
Published: March 2023

Abstract
Welding a combination of hard-to-weld materials such as cemented carbides WC-Co and tool steel X153CrMoV12 is a big challenge due to the different properties of the materials being joined. The solution can be the method of multicapacitor discharge welding, which allows high temperatures to be reached for very short times. The weld was formed continuously around the entire circumference. It has been proven that, during welding, both materials melt and mix in the joining zone. A melting zone depleted of chromium is formed.
Cluster of variations involving vertebral, renal, and obturator arteries in a single human body- a cadaveric case report.
Authors: G Sankaranarayanan , SSSN Rajasekhar , K Kalaivani 
Published: March 2023

Abstract
We describe a unique triad of arterial variations that include vertebral, renal, and obturator arteries in a single cadaver. A combination of arterial variations was observed in a 60-year male; formalin embalmed cadaver during the routine dissection while teaching undergraduate students. The origin, course, branching pattern, and termination of the vertebral, renal, and obturator arterial systems were noted, along with the vessels' diameter close to their origin. The following variations were observed: a) Hypoplastic V4 segment of the right vertebral artery; Right Posterior Inferior Cerebellar artery had a relatively proximal origin from the V4 segment of vertebral artery and coursed in an inverted ‘U’ shaped manner cranially, which resembled duplicated V4 segment of the right vertebral artery, b) Right accessory renal artery originated from abdominal aorta distal to the right renal artery and the right gonadal artery originated from the right accessory renal artery, c) Right obturator artery originated from the posterior division of the right internal iliac artery, and the left obturator artery originated from the trunk of the left external iliac artery. Such a combination of variations in the anatomy of vertebral, renal, and obturator arteries was not reported previously. This case report will interest neurosurgeons and radiologists because of the possibility of concomitant arterial variations.
Eye movement defects in KO zebrafish reveals SRPK3 as a causative gene for an X-linked intellectual disability
Authors: Kim Cheol-Hee , Lee Yu-Ri , Thomas Mervyn , Roychaudhury Arkaprava , Skinner Cindy , Maconachie Gail , Crosier Moira , Horak Holli , Constantinescu Cris , Choi Tae-Ik , Kyung Jae-Jun , Wang Tao , Ku Bonsu , Chodirker Bernard , Hammer Michael , Gottlob Irene , Norton William , Chudley Albert , Schwartz Charles 
Published: March 2023

Abstract
Intellectual disability (ID) is a common neurodevelopmental disorder characterized by significantly impaired intellectual and adaptive functioning. X-linked ID (XLID) disorders, caused by defects in genes on the X chromosome, affect 1.7 out of 1,000 males. Employing exome sequencing, we identified three missense mutations (c.475C > G; p.H159D, c.1373C > A; p.T458N, and c.1585G > A; p.E529K) in the SRPK3 gene in seven XLID patients from three independent families. Clinical features common to the patients are intellectual disability, agenesis of the corpus callosum, abnormal smooth pursuit eye movement, and ataxia. SRPK proteins are known to be involved in mRNA processing and, recently, synaptic vesicle and neurotransmitter release. In order to validate SRPK3 as a novel XLID gene, we established a knockout (KO) model of the SRPK3 orthologue in zebrafish. In day 5 of larval stage, KO zebrafish showed significant defects in spontaneous eye movement and swim bladder inflation. In adult KO zebrafish, we found agenesis of cerebellar structures and impairments in social interaction. These results suggest an important role of SRPK3 in eye movements, which might reflect learning problems, intellectual disability, and other psychiatric disorders.
The Effect of Wobbling on the Welding Characteristics in Al/Cu Fiber Laser Welded Joints
Authors: Yoon HyeSeul , Bang Hee Seon 
Published: March 2023

Abstract
The IR laser welding of aluminum and copper materials for lithium-ion battery cells has limitation due to unsatisfactory joints strength caused by their low absorptivity and high reflectivity. To overcome these problems, this study has applied beam oscillation to increase the joint area of welded joints. A 1000 series aluminum alloy and oxygen-free copper sheets with a thickness of 0.5 mm were lap joints welded through 2.5 kW fiber laser welding. The effect of the welding parameters of amplitudes (0, 0.2, 0.4, 0.6 mm) on weldability in welded joints has been investigated. The result confirmed that as the amplitude increases, the width at the interface increases, and penetration depth decreases. Furthermore, the maximum width at the interface could be obtained at approximately 1.23 mm under a travel speed of 100 mm/sec and amplitude of 0.6 mm. In addition, the tensile-shear load increased with higher amplitudes, and the maximum tensile-shear load was 1.1 kN in the amplitude of 0.6 mm with a travel speed of 400 mm/sec.
Evaluating Compliance and Applicability of Postpartum Pessary Use for Preventing and Treating Pelvic floor Dysfunction: A Prospective Multicenter Study
Authors: Kiefner Brenda , Schwab Frank , Kuppinger Madeleine , Nacke Anna , Kelkenberg Ute , Schütze Sabine , Berger Franziska , Lindner Anna , Hellmeyer Lars , Janni Wolfgang , Metz Melanie , Deniz Miriam 
Published: March 2023

Abstract
Purpose: Pelvic floor disorders are common and associated with pregnancy and childbirth. For restitution of pelvic floor connective tissue and thereby therapy of postpartum pelvic organ prolapse and stress-urinary incontinence the Restifem® pessary is approved. It supports the anterior vaginal wall behind the symphysis, the lateral sulci and the sacrouterinum ligaments and stabilises the connective tissue. We evaluated the compliance and applicability of Restifem® use in women postpartum in a preventive and therapeutic approach.
Methods: Restifem® pessary was handed out to 857 women. 6 weeks after birth they started the pessary use. After 8 weeks, 3- and 6-months postpartum women received a questionnaire via online survey for evaluation of pessary applicability and efficacy.
Results: After 8 weeks 209 women answered the questionnaire. 119 women used the pessary. Common problems were discomfort, pain and the pessary use was to circuitous. Vaginal infections were rare. After 3 months 85 women and after 6 months 38 women still used the pessary. 3 months postpartum 94 % of women with POP, 72 % of women with UI and 66 % of women with OAB stated to have an improvement of their symptoms by using the pessary. 88 % women without any disorder felt an improvement of stability.
Conclusions: Use of the Restifem® pessary in the postpartum period is feasible and accompanied with less complications. It reduces POP and UI and leads to an increased sense of stability. So, Restifem® pessary can be offered to women postpartum to improve pelvic floor dysfunction.
ThGM-CSF cells contribute to the protective immune response against Listeria monocytogenes
Authors: Cheng Hongyan , Cheng Dalei , Li Zhihua , Zhao Xiyu , Zhu Yuanbo , Zhang Hui , Yan Fenglian , Jing Lina , Zhang Xin , Wang Lin , Wang Changying , Xiong Huabao 
Published: March 2023

Abstract
Aim To investigate the functions of ThGM-CSF cells in the host defense against L. monocytogenes infection.
Material 168 Wild-type (WT, C57BL/6J) mice and 18 GM-CSF deficient (Csf2−/−) mice were used in this study. In vitro bone marrow-derived macrophages (BMDMs) and ThGM-CSF cells were respectively derived from bone marrow and spleen.
Methods A protective immune mouse model was established with L. monocytogenes. Flow cytometry, enzyme-linked immunosorbent assay, and quantitative RT-PCR were used to detect the expression of related immune cells and proteins at cellular, protein and mRNA levels. Plate counts and immunofluorescence were used to determine bacterial colonization.
Results ThGM-CSF cells contribute to the protective immune response against L. monocytogenes infection. The induction of GM-CSF is significantly increased in both primary and secondary infection. Csf2−/− mice are more susceptible to L. monocytogenes infection and transfer of ThGM-CSF cells enhanced the clearance of L. monocytogenes. Anti-GM-CSF neutralizing antibody impaired the elimination of L. monocytogenes and the generation of protective immune response. Depletion of macrophages and neutrophils decreased ThGM-CSF cells development. GM-CSF promotes phagocytosis of macrophages and neutrophils to clear L. monocytogenes.
Conclusion The results suggest that ThGM-CSF cells are significantly involved in the generation of protective immune responses against bacterial infection.
Characterization of heavy metal-resistant rhizobia and non-rhizobia isolated from root nodules of Trifolium sp. in a lead and zinc mining area
Authors: rahal sarah , Menaa Belkis , Chekireb Djamel 
Published: March 2023

Abstract

In this study, we evaluated the ability of rhizobacteria isolated from Trifolium sp. nodules growing on a Pb/Zn mine site to produce plant growth-promoting substances, such as siderophores, extracellular enzymes, antifungal substances, their capacity to solubilize phosphate, and also their tolerance to heavy metals and salinity. Overall, the results demonstrated that bacterial isolates showed an ability to produce multiple important plant growth-promoting traits, with remarkable ability to grow up to 20% salt concentration and resist to high levels of heavy metals up to 1300 mg/L Pb++, 1200 mg/L Zn++, 1000 mg/L Ni++, 1000 mg/L Cd++, 500 mg/L Cu++, 400 mg/L Co++, and 50 mg/L CrVI+. The order of resistance of isolates to heavy metals was reduced as follows: Pb++ > Zn++ > Ni++ > Cd++ > Cu++ > Co++ > CrVI+. All isolates had multiple metal-resistant abilities; however, the existence of the pbrA, czcD, and nccA genes responsible for resistance to Pb++, Zn++, Cd++, Co++, and Ni++, respectively, was determined by PCR and were detected only on Cupriavidus paucula RSCup01. Our results also showed that the plant growth-promoting rhizobacteria strains screened in the present study could be used as a potential inoculant for the improvement of phytoremediation in heavy metal-polluted soils.
Sequential Mergers and Delayed Monopolization in Triopoly
Authors: LAUSSEL DIDIER 
Published: March 2023

Abstract
Under triopoly and Cournot competition, we study an infinite horizon Markov Perfect Equilibrium merger game in which in each period one of the firms (”the Buyer”) selects a bid price and then the two sellers accept or reject this offer with some probability. The possibility of a ”war of attrition” equilibrium in which the seller who outlasts the other one is then able to sell in the following period at a greater price, is a distinct feature of the model. Delayed monopolization is all the more likely as the discount factor is small and the ratio duopoly/ triopoly profits.is important. Two other equilibria are shown to be possible: an unmerged one and an immediate monopolization one. Each equilibrium is shown to correspond to a different set of parameter values. The two special cases of linear and constant price elastic demand functions are fully characterized.
JEL: C73, D43, L12
Group intervention for family members of people with borderline personality disorder based on Dialectical Behavior Therapy: Implementation of the Family Connections® program in France and Switzerland
Authors: Cohen Satchel , Salamin Virginie , Perroud Nader , Dieben Karen , Ducasse Deborah , Durpoix Amaury , Guenot Florence , Tissot Hervé , Kramer Uëli , Speranza Mario 
Published: March 2023

Abstract

BACKGROUND Families and close relationships of people living with borderline personality disorder (BPD) show increased levels of psychological distress. Family Connections®, a 12-week group intervention based on the principles of Dialectical Behavioral Therapy, has been designed to provide families with both information about the disorder and emotional regulation skills. It has been progressively implemented in French-speaking European countries.
METHODS We conducted an observational, multicenter study in France and Switzerland. 149 participants to the Family Connections program were included among 5 centers. Burden, depression, coping, and emotional regulation were assessed before and after intervention.
RESULTS T-tests showed that burden significantly decreased after intervention (p<0.0001, d=-0.48), as did depressive symptoms (p<0.001, d=-0.36). Emotional regulation (p<0.0001, d=0.32) and coping (p<0.0001, d=0.53) improved. Two-way mixed ANOVA showed that burden reduction was stronger among women participants compared to men participants (p=0.048, η²=0.027). Before intervention, burden was higher for women participants (p=0.001) than for men. A first linear regression showed that reduction of burden was associated with decrease in resignation of participants (β=0.19, p=0.047). A second linear regression showed that reduction of burden was associated with intensity of the loved one’s symptoms and improvement of emotional clarity of participants (β=0.25, p=0.008).
CONCLUSION This Dialectical Behavioral Therapy-Based psychoeducational intervention is an appropriate way to support French-speaking European families of people with BPD.
Functional expression of the ATP-gated P2X7 receptor in human iPSC-derived astrocytes
Authors: Kesavan Jaideep , Watters Orla , Diego-Garcia Laura , Mendez Aida Menendez , Alves Mariana , Dinkel Klaus , Hamacher Michael , Prehn Jochen H. M. , Henshall David C. , Engel Tobias 
Published: March 2023

Abstract

The P2X7 receptor (P2X7R) is a cation-permeable ionotropic receptor activated by extracellular adenosine 5’-triphosphate (ATP) which has been implicated in numerous diseases of the CNS, including epilepsy. Activation of the P2X7R can trigger diverse responses including the release of pro-inflammatory cytokines, modulation of neurotransmission, cell proliferation or cell death. There have been conflicting reports on the cellular identity of P2X7R-expressing cells in the brain. Expression of P2X7Rs is well documented on microglia and oligodendrocytes but the presence of P2X7Rs on astrocytes remains debated. Furthermore, most functional studies on P2X7R responses have used cells from rodents or immortalised cell lines expressing human P2X7Rs. To assess the endogenous and functional expression of P2X7Rs in human astrocytes, we differentiated human-induced pluripotent stem cells (hiPSCs) into GFAP and S100 β-expressing astrocytes. Immunostaining revealed prominent punctate P2X7R staining on hiPSC-derived astrocytes and P2X7R protein expression was also confirmed by Western blot analysis. Importantly, stimulation with the potent nonselective P2X7R agonist BzATP or endogenous agonist ATP induced robust calcium rises in hiPSC-derived astrocytes which were blocked by the selective P2X7R antagonists AFC-5128 or JNJ-47965567. Our findings provide evidence for the functional expression of P2X7Rs in hiPSC-derived astrocytes and support their in vitro utility in investigating the role of the P2X7R and drug screening in disorders of the CNS.
Natural wetlands in protected areas as a key to water quality resilience
Authors: Benegas Gabriela Sosa , Imas-Garay Lourdes , Netto Flavia , Lombardo Laura , Vergara Angela , Martínez Karen , Céspedes Rosa , Vera Giselle , Mendez-Gaona Sergio , Sosa Paola Inchausti , Gossen Ana Carolina , Bareiro Gustavo 
Published: March 2023

Abstract
Wetlands are buffers for terrestrial runoff with the essential service of water purification. Despite its importance, they are experiencing degradation due to the combined effect of climate change and poor land-use practices. Studies of natural wetlands in Paraguay are scarce. We assessed wetland water quality conditions using pH, turbidity, total phosphorus (TP), and bacterial indicators of 12 natural wetlands located in protected areas (PW) or in the border of agricultural lands (AW) in the Paraguayan side of the watershed of Parana River. AW presented acidic pH values (x̄=5.5). We registered higher TP values in AW (x̄=0.14 mg/L) compared to PW (x̄=0.06 mg/L). For turbidity AW presented higher and variable values (x̄=425 NTU) than PW (x̄=34 NTU). In connected wetlands as the nutrient flows through the wetlands system to the reservoir concentration decreased 70% (0.1 to 0.03 mg/L) and the turbidity decreased from 112 to 42,6 NTU. This study observed a high degree of variability of bacterial indicators in water and sediment. The counts of coliforms in water and sediment samples were in the order E.Coli>Total coliforms>Fecal Coliforms. Long term contamination by organophosphorus and carbamate pesticides was pointed by the positive results in 100% of the sediment samples. As far as we can conclude wetlands has a key function in ecosystem function, health and preserving the water quality that enters to the reservoir, and protected PW presented better water quality conditions in terms of TP, turbidity, and bacterial indicators.
Posterior Inference of Differential Expression (DE) State and Evolutionary Distance
Authors: Gu Xun 
Published: March 2023

Abstract
To address how gene regulation plays a key role in phenotypic innovations through high throughput transcriptomes, it is desirable to develop statistically-sound methods that enable researchers to study the pattern of transcriptome evolution. On the other hand, statistical detection of differentially expressed (DE) genes between two samples is probably the most common practice in the transcriptome analysis. In this paper, the author developed a new evolutionary approach, based on the genome-wide p-value profile arising from statistical testing of DE genes between species. First, the widely-used BUM (beta-uniform mixture) model for the genome-wide p-value distribution was invoked to calculate the posterior probability of a gene being DE denoted by Q = P(DE|p). Then the author develop a simple method to estimate the transcriptome distance for any number (m) of orthologous genes based on their Q-values. Computer simulations showed the statistical performance of these new methods are generally satisfactory.
A weightless factor two-stage model predictive control strategy
Authors: Wang Guifeng , Li Peiru , Wang Yu 
Published: March 2023

Abstract
In this paper, a weightless factor two-stage finite set model predictive control (FCS-MPC) strategy is proposed to drive asynchronous motors with three-level Neutral Point Clamped Voltage Source Inverter (3L-NPC-VSI) as the research object, in order to achieve weightless factor control and reduce torque ripple. Firstly, in view of the difficulty of weight factor setting in traditional FCS-MPC applications, the layered optimization method is adopted to stratify the three performance indicators of voltage trip, common-mode voltage and current follow-up, so as to avoid the weight factor setting link and reduce the amount of prediction operation. Secondly, in order to further reduce the torque ripple, in the current control layer to the current following error as the principle of the least, the role of an optimal vector or optimal vector combination, and in the optimal vector combination selection process, the first vector of the combination is selected as the vector at the end of the current cycle, so as to ensure that there is only one switching state switch in each control cycle, reducing the switching loss of the two-stage FCS-MPC. Finally, simulation and experimental results verify the feasibility and effectiveness of the proposed strategy.
Regulation of Platform Price Discrimination on Either or Both Sides of the Market
Authors: Lestage Romain , Kim Soo Jin 
Published: March 2023

Abstract
We analyze the welfare effects of price discrimination by a monopolistic platform that mediates between two sides of a market. Discrimination is based on asymmetric costs on one side of the market and may be allowed on either side or both sides. We show that unconstrained discrimination on both sides of the market improves welfare compared to uniform pricing but may be dominated by single-side discrimination. This result is driven by matching effects found between the sides, which add to the standard reallocation effects found within the sides.
JEL Classification Numbers: D4, L1
Intervention analysis for integer-valued autoregressive models
Authors: Pedeli Xanthi , Fried Roland 
Published: March 2023

Abstract
We study the problem of intervention effects generating various types of outliers in an integer-valued autoregressive model with Poisson innovations. We concentrate on outliers which enter the dynamics and can be seen as effects of extraordinary events. Weconsider three different scenarios, namely the detection of an intervention effect of a known type at a known time, the detection of an intervention effect of unknown type at a known time andthe detection of an intervention effect when both the type and the time are unknown. We develop \(F\) -tests and score tests for the first scenario. For the second and third scenarios we rely on the maximum of the different $F$-type or score statistics. The usefulness of the proposed approach is illustrated using monthly data on human brucellosis infections in Greece.