bluuebunny/crossref_metadata_2025_split huggingface dataset:
Appropriate procedures to increase the adherence of children to blood collection: A cross‐sectional study
Authors: Yui Hideki , Otawa Sanae , Horiuchi Sayaka , Kushima Megumi , Shinohara Ryoji , Kojima Reiji , Akiyama Yuka , Ooka Tadao , Miyake Kunio , Yokomichi Hiroshi , Yamagata Zentaro ,   The Yamanashi Adjunct Study of the Japan Environment and Children's Study Group
Published: December 2022

AbstractBackground and AimVenipuncture for blood collection elicits fear and pain in children. We investigated factors that affect satisfaction with health checkups that included blood collection in healthy 7–8‐year‐old children who underwent blood collection with topical anesthesia.MethodTwo studies, one questionnaire survey, and the other structured interviews were conducted to gather insights and understand the emotions of 492 and 20 children, respectively.ResultsWe found that the following six points can be applied to encourage children to assess their experience of blood collection positively: (1) prior information using a pamphlet; (2) telling the children that the volume of blood drawn will be small; (3) carefully explaining the risk and benefit of topical anesthesia; (4) conducting the blood collection process swiftly; (5) praising and thanking the children's effort and cooperation; and (6) explaining the results of the research to the children if their blood is going to be used for research.ConclusionThe findings indicate that with appropriate measures to reduce pain and fear, children's initial negative feelings toward blood collection can be replaced by positive feelings after the procedure.
Modelling snowpack bulk density using snow depth, cumulative degree‐days, and climatological predictor variables
Authors: Szeitz Andras J. , Moore R. Dan 
Published: December 2022

AbstractSnowpack water equivalent (SWE) is a key variable for water resource management in snow‐dominated catchments. While it is not feasible to quantify SWE at the catchment scale using either field surveys or remotely sensed data, technologies such as airborne LiDAR (light detection and ranging) support the mapping of snow depth at scales relevant to operational water management. To convert snow depth to water equivalent, models have been developed to predict SWE or snowpack density based on snow depth and additional predictor variables. This study builds upon previous models that relate snowpack density to snow depth by including additional predictor variables to account for (1) long‐term climatologies that describe the prevailing conditions influencing regional snowpack properties, and (2) the effect of intra‐ and inter‐year variability in meteorological conditions on densification through a cumulative degree‐day index derived from North American Regional Reanalysis products. A non‐linear model was fit to 114 506 snow survey measurements spanning 41 years from 1166 snow courses across western North America. Under spatial cross‐validation, the predicted densities had a root‐mean‐square error of 47.1 kg m−3, a mean bias of −0.039 kg m−3, and a Nash‐Sutcliffe Efficiency of 0.70. The model developed in this study had similar overall performance compared to a similar regression‐based model reported in the literature, but had reduced seasonal biases. When applied to predict SWE from simulated depths with random errors consistent with those obtained from LiDAR or Structure‐from‐Motion, 50% of the SWE estimates for April and May fell within −45 to 49 mm of the observed SWE, representing prediction errors of −15% to 20%.
Functional recovery of myocardial infarction by specific EBP‐PR1P peptides bridging injectable cardiac extracellular matrix and vascular endothelial growth factor
Authors: Cao Wenxuan , Zhang Hong , Zhou Ning , Zhou Runxue , Zhang Xiaojing , Yin Jia , Deng Jin , Ao Xiang , Shi Chunying 
Published: December 2022

AbstractVascular endothelial growth factor (VEGF) is the most potent angiogenic factor and plays an important role in therapy of myocardial infarction (MI). Currently, how to retain regional concentration and decrease rapid diffusion is critical for its clinical application of VEGF. In recent years, the application of targeting peptides has been developed rapidly and provides new strategies for the sustained release of VEGF. In present study, a bi‐functional EBP‐PR1P peptide was designed and bridged VEGF to injectable cardiac extracellular matrix (c‐ECM). Through EBP‐PR1P peptides, VEGF could specifically bind with c‐ECM to realize the sustained release, without impacting the bioactivity of VEGF. Then VEGF/EBP‐PR1P/c‐ECM scaffolds were constructed and administrated into rats with MI. The results showed VEGF/EBP‐PR1P/c‐ECM could promote angiogenesis, protect cardiomyocytes survival against apoptosis, and improve the recovery of cardiac function. In addition, the mechanism of EBP‐PR1P/VEGF was also investigated which canonical downstream of VEGF‐Akt signaling pathway was activated. These results showed specific VEGF/EBP‐PR1P/c‐ECM scaffolds served as promising delivery system for VEGF that facilitated the functional recovery of MI.
Interaction energy of Cl2 and Br2 with H2O: Exchange, dispersion and density the crucial ingredients
Authors: Morera‐Boado Cercis , Bernal‐Uruchurtu Margarita I. 
Published: December 2022

AbstractModern Density Functional Theory models are now suitable for many molecular and condensed phase studies. The study of noncovalent interactions, a well‐known drawback, is no longer an insurmountable obstacle through design and empirical corrections. However, using empirical corrections as in the DFT‐D methods might not be an all‐in‐one solution. This work uses a simple system, X2‐H2O with X = Cl or Br, with two different interactions, halogen‐bonded (XB) and hydrogen‐halogen (HX), to investigate the capability of current density functional approximations (DFA) in predicting interaction energies with eight different exchange‐correlation functionals. SAPT(DFT) provides, for all the studied cases, better predictions than the widely used supermolecular approach. In addition, the components of the interaction energy suggest where some of the shortcomings originate in each DFA. The analysis of the functionals used confirms that PBE0 and ω‐B97X‐D have a physically correct behavior. Using SAPT(DFT) and PBE0, and ω‐B97X‐D, we obtained the interaction energy of Cl2 and Br2 inside different clathrate cages and satisfactorily compared with wavefunction results; hence, the lower and upper limits of this value are defined: Cl2@512, −5.3 ± 0.3 kcal/mol; Cl2@51262, −5.5 ± 0.1 kcal/mol; Br2@51262, −7.6 ± 1.0 kcal/mol; Br2@51263, −10.6 ± 1.0 kcal/mol; Br2@51264, −10.9 ± 0.8 kcal/mol.
Clinical value of serum AFP and PIVKA‐II for diagnosis, treatment and prognosis of hepatocellular carcinoma
Authors: Tian Shan , Chen Yongyi , Zhang Yimin , Xu Xiaohong 
Published: December 2022

AbstractBackgroundThe accuracy of alpha‐fetoprotein (AFP) as a diagnostic marker for hepatocellular carcinoma (HCC) is insufficient, and the application of abnormal prothrombin (PIVKA‐II) in HCC is still controversial.MethodsSerum AFP and PIVKA‐II levels were analyzed in 145 cases of HCC, 57 of benign liver disease, 55 of cholangiocarcinoma and gallbladder carcinoma, 112 of other gastrointestinal tumors with liver metastasis, and 101 healthy controls. Receiver operating characteristic curve and area under the curve were used to evaluate the diagnostic value of AFP and PIVKA‐II for HCC. The changes in serum AFP and PIVKA‐II levels before and after treatment in 47 HCC patients who underwent surgery and 77 who received interventional treatment were used to evaluate treatment efficacy and prognosis in HCC.ResultsThe concentrations of AFP and PIVKA‐II in the HCC group were significantly higher than those in other groups (p < 0.01). The diagnostic value of PIVKA‐II in HCC was better than that of AFP, and combined detection improved the diagnostic sensitivity and specificity. The levels of AFP and PIVKA‐II after liver cancer surgery were significantly lower than those before surgery. Elevated levels of PIVKA‐II before surgery predicted disease progression, and patients who remained positive for PIVKA‐II after surgery had worse prognosis than those who became negative after surgery.ConclusionsCombined detection of AFP and PIVKA‐II is superior to both tests alone. We found that higher serum level of PIVKA‐II indicates more severe HCC, with worse prognosis, while the level of AFP had no correlation with the prognosis.
Multifaceted functions of RPS27a: An unconventional ribosomal protein
Authors: Luo Jingshun , Zhao Hong , Chen Linxi , Liu Meiqing 
Published: December 2022

AbstractThe ribosomal protein S27a (RPS27a) is cleaved from the fusion protein ubiquitin–RPS27a (Ub–RPS27a). Generally, Ub and RPS27a are coexpressed as a fusion protein but function independently after Ub is cleaved from RPS27a by a deubiquitinating enzyme. As an RP, RPS27a assembles into ribosomes, but it also functions independently of ribosomes. RPS27a is involved in the development and poor prognosis of various cancers, such as colorectal cancer, liver cancer, chronic myeloid leukemia, and renal carcinoma, and is associated with poor prognosis. Notably, the murine double minute 2/P53 axis is a major pathway through which RPS27a regulates cancer development. Moreover, RPS27a maintains sperm motility, regulates winged aphid indirect flight muscle degeneration, and facilitates plant growth. Additionally, RPS27a is a metalloprotein and mercury (Hg) biomarker. In the present review, we described the origin, structure, and biological functions of RPS27a.
Peripheral blood cells of native pig (Zovawk) of Mizoram, India: Light and scanning electron microscopy analysis
Authors: Choudhary Om Prakash , Sarkar Rupan , Madkour Fatma A. , Kalita Pranab Chandra , Doley Probal Jyoti , Kalita Arup , Choudhary Priyanka , Eregowda Chethan Gollahalli 
Published: December 2022

AbstractThe literature on the blood cells of Zovawk pigs is scanty; thus, this study was designed to elaborate the light microscopy (LM) and scanning electron microscopy (SEM) characterization of blood cells of Zovawk pigs by LM and SEM. Twelve different blood samples were aseptically obtained from adult healthy Zovawk pigs, irrespective of sex. To determine the cytomorphological and cytochemical structures of the many produced constituents of blood, blood smears were stained with various stains. The blood samples were treated with various substrates for cytoenzymatic research, and the alterations were noted. A 1000× magnification Olympus Trinocular Research microscope was used to examine the smears. The blood samples were prepared for electron microscopy according to the standard procedure. The prepared samples were delivered to the Sophisticated Analytical Instrumentation Facility (SAIF), All India Institute of Medical Sciences (AIIMS), New Delhi, for SEM imaging. On LM, pig erythrocytes were spherical and nonnucleated. The cytoplasm of the neutrophils was spherical and included cytoplasmic granules. The eosinophils had prominent cytoplasmic granules and were round. Basophils were infrequently present and had cytoplasmic granules that were clear blue. The sizes of small, medium and large lymphocytes were noted. The monocytes were oval or circular. The platelets ranged in form from asymmetric to round. The blood samples were stained for cytochemical analyses using acid ferrocyanide stain for iron, Sudan black blue stain for lipids, toluidine blue stain for mucopolysaccharides, and periodic acid Schiff's stain for glycogen. The cytoenzymatic characteristics were evaluated and compared with substrates treated with acid phosphatase, alkaline phosphatase, peroxidase, arylsulfatase, cytochrome oxidase, beta‐glucuronidase, and succinate dehydrogenase. Erythrocytes appeared as biconcave disks under SEM. Two forms of leukocytes were observed, having a rough and pointed cell surface like a flower. It can be concluded that the LM and SEM morphology of blood cells of Zovawk pigs resembled other domestic animals, however, few differences were observed among the discussed animals.
Severity of acute pancreatitis in patients with inflammatory bowel disease in the era of biologics: A propensity‐score‐matched analysis using a nationwide database in Japan
Authors: Moroi Rintaro , Tarasawa Kunio , Ikeda Mio , Matsumoto Ryotaro , Shimoyama Yusuke , Naito Takeo , Takikawa Tetsuya , Shiga Hisashi , Hamada Shin , Kakuta Yoichi , Kikuta Kazuhiro , Fushimi Kiyohide , Fujimori Kenji , Kinouchi Yoshitaka , Masamune Atsushi 
Published: December 2022

AbstractBackground and AimAcute pancreatitis (AP) is a rare extraintestinal manifestation of inflammatory bowel disease (IBD). Several studies from Western countries have reported that the severity of AP in patients with IBD is similar to that in the general population; however, its severity in patients from Eastern countries in the era of biologics remains unclear. This study aimed to investigate the severity of AP in patients with IBD and the effect of biologics on the severity of AP using a nationwide database.MethodsWe divided 1138 eligible AP admissions from the Diagnosis Procedure Combination database system into IBD and non‐IBD groups after propensity score matching, and compared the severity of AP. We divided the IBD group into ulcerative colitis (UC) and Crohn's disease (CD) subgroups and compared each with the non‐IBD group. Logistic regression analysis was conducted to identify the clinical factors affecting acute pancreatitis.ResultsIBD and UC groups had lower rate of severe AP compared to the non‐IBD group (13.7% vs 28.3%, P < 0.0001 and 11.0% vs 28.3%, P < 0.0001, respectively). There were no differences in the rates of severe AP between the CD and non‐IBD groups. Multivariate analysis showed that biologics did not affect the severity of AP.ConclusionThe severity of AP in patients with IBD may be lower than that in the general population; biologics for IBD may not worsen its severity. Further prospective studies are required to clarify the severity of AP in patients with IBD.
Distinguishing threshold of graphs
Authors: Shekarriz Mohammad H. , Ahmadi Bahman , Talebpour S. A. , Shirdareh Haghighi M. H. 
Published: December 2022

AbstractA vertex coloring of a graph is called distinguishing if no nonidentity automorphisms of can preserve it. The distinguishing number of , denoted by , is the minimum number of colors required for such a coloring, and the distinguishing threshold of , denoted by , is the minimum number such that every ‐coloring of is distinguishing. As an alternative definition, is one more than the maximum number of cycles in the cycle decomposition of automorphisms of . In this paper, we characterize when is disconnected. Afterwards, we prove that, although for every positive integer there are infinitely many graphs whose distinguishing thresholds are equal to , we have if and only if . Moreover, we show that if , then either is isomorphic to one of the four graphs on three vertices or it is of order , where is a prime number. Furthermore, we prove that if and only if is asymmetric, or . Finally, we consider all generalized Johnson graphs, , which are the graphs on all ‐subsets of where two vertices and are adjacent if . After studying their automorphism groups and distinguishing numbers, we calculate their distinguishing thresholds as , unless and in which case we have .
Classification of Major Depressive Disorder Based on Integrated Temporal and Spatial Functional MRI Variability Features of Dynamic Brain Network
Authors: Gai Qun , Chu Tongpeng , Che Kaili , Li Yuna , Dong Fanghui , Zhang Haicheng , Li Qinghe , Ma Heng , Shi Yinghong , Zhao Feng , Liu Jing , Mao Ning , Xie Haizhu 
Published: December 2022

BackgroundCharacterization of the dynamics of functional brain network has gained increased attention in the study of depression. However, most studies have focused on single temporal dimension, while ignoring spatial dimensional information, hampering the discovery of validated biomarkers for depression.PurposeTo integrate temporal and spatial functional MRI variability features of dynamic brain network in machine‐learning techniques to distinguish patients with major depressive disorder (MDD) from healthy controls (HCs).Study TypeProspective.PopulationA discovery cohort including 119 patients and 106 HCs and an external validation cohort including 126 patients and 124 HCs from Rest‐meta‐MDD consortium.Field Strength/SequenceA 3.0 T/resting‐state functional MRI using the gradient echo sequence.AssessmentA random forest (RF) model integrating temporal and spatial variability features of dynamic brain networks with separate feature selection method (MSFS) was implemented for MDD classification. Its performance was compared with three RF models that used: temporal variability features (MTVF), spatial variability features (MSVF), and integrated temporal and spatial variability features with hybrid feature selection method (MHFS). A linear regression model based on MSFS was further established to assess MDD symptom severity, with prediction performance evaluated by the correlations between true and predicted scores.Statistical TestsReceiver operating characteristic analyses with the area under the curve (AUC) were used to evaluate models' performance. Pearson's correlation was used to assess relationship of predicted scores and true scores. P < 0.05 was considered statistically significant.ResultsThe model with MSFS achieved the best performance, with AUCs of 0.946 and 0.834 in the discovery and validation cohort, respectively. Additionally, altered temporal and spatial variability could significantly predict the severity of depression (r = 0.640) and anxiety (r = 0.616) in MDD.Data ConclusionIntegration of temporal and spatial variability features provides potential assistance for clinical diagnosis and symptom prediction of MDD.Evidence Level2.Technical EfficacyStage 2.
Surveillance for SARS‐CoV‐2 and its variants in wastewater of tertiary care hospitals correlates with increasing case burden and outbreaks
Authors: Acosta Nicole , Bautista Maria A. , Waddell Barbara J. , Du Kristine , McCalder Janine , Pradhan Puja , Sedaghat Navid , Papparis Chloe , Beaudet Alexander Buchner , Chen Jianwei , Van Doorn Jennifer , Xiang Kevin , Chan Leslie , Vivas Laura , Low Kashtin , Lu Xuewen , Lee Jangwoo , Westlund Paul , Chekouo Thierry , Dai Xiaotian , Cabaj Jason , Bhatnagar Srijak , Ruecker Norma , Achari Gopal , Clark Rhonda G. , Pearce Craig , Harrison Joe J. , Meddings Jon , Leal Jenine , Ellison Jennifer , Missaghi Bayan , Kanji Jamil N. , Larios Oscar , Rennert‐May Elissa , Kim Joseph , Hrudey Steve E. , Lee Bonita E. , Pang Xiaoli , Frankowski Kevin , Conly John , Hubert Casey R. J. , Parkins Michael D. 
Published: December 2022

AbstractWastewater‐based SARS‐CoV‐2 surveillance enables unbiased and comprehensive monitoring of defined sewersheds. We performed real‐time monitoring of hospital wastewater that differentiated Delta and Omicron variants within total SARS‐CoV‐2‐RNA, enabling correlation to COVID‐19 cases from three tertiary‐care facilities with >2100 inpatient beds in Calgary, Canada. RNA was extracted from hospital wastewater between August/2021 and January/2022, and SARS‐CoV‐2 quantified using RT‐qPCR. Assays targeting R203M and R203K/G204R established the proportional abundance of Delta and Omicron, respectively. Total and variant‐specific SARS‐CoV‐2 in wastewater was compared to data for variant specific COVID‐19 hospitalizations, hospital‐acquired infections, and outbreaks. Ninety‐six percent (188/196) of wastewater samples were SARS‐CoV‐2 positive. Total SARS‐CoV‐2 RNA levels in wastewater increased in tandem with total prevalent cases (Delta plus Omicron). Variant‐specific assessments showed this increase to be mainly driven by Omicron. Hospital‐acquired cases of COVID‐19 were associated with large spikes in wastewater SARS‐CoV‐2 and levels were significantly increased during outbreaks relative to nonoutbreak periods for total SARS‐CoV2, Delta and Omicron. SARS‐CoV‐2 in hospital wastewater was significantly higher during the Omicron‐wave irrespective of outbreaks. Wastewater‐based monitoring of SARS‐CoV‐2 and its variants represents a novel tool for passive COVID‐19 infection surveillance, case identification, containment, and potentially to mitigate viral spread in hospitals.
Peptidyl arginine deiminase 4 deficiency protects against subretinal fibrosis by inhibiting Müller glial hypercitrullination
Authors: Palko Sarah I. , Saba Nicholas J. , Bargagna‐Mohan Paola , Mohan Royce 
Published: December 2022

AbstractRetinal scarring with vision loss continues to be an enigma in individuals with advanced age‐related macular degeneration (AMD). Müller glial cells are believed to initiate and perpetuate scarring in retinal degeneration as these glial cells participate in reactive gliosis and undergo hypertrophy. We previously showed in the murine laser‐induced model of choroidal neovascularization that models wet‐AMD that glial fibrillary acidic protein (GFAP) expression, an early marker of reactive gliosis, increases along with its posttranslational modification citrullination. This was related to increased co‐expression of the citrullination enzyme peptidyl arginine deiminase‐4 (PAD4), which also colocalizes to GFAP filaments. However, whether such hypercitrullination in Müller glial drives fibrotic pathology has remained understudied. Here, using male and female C57Bl6 mice subjected to laser injury, we investigated in a temporal study how citrullination impacts GFAP and PAD4 dynamics. We found that high molecular weight citrullinated species that accumulate in Müller glia corresponded with dynamic changes in GFAP and PAD4 showing their temporal redistribution from polymeric cytoskeletal to soluble protein fractions using immunostaining and western blot analysis. In conditional glial‐specific PAD4 knockout (PAD4cKO) mice subjected to laser injury, there was a stark reduction of citrullination and of polymerized GFAP filaments. These injured PAD4cKO retinas showed improved lesion healing, as well as reduced fibronectin deposition in the subretinal space at 30 days. Taken together, these findings reveal that pathologically overexpressed PAD4 in reactive Müller glia governs GFAP filament dynamics and alters their stability, suggesting chronic PAD4‐driven hypercitrullination may be a target for retinal fibrosis.