bluuebunny/crossref_metadata_2025_split huggingface dataset:
Exploring the Synergy of Metallic Antimicrobial Agents in Ternary Blends of PHB/PLA/PCL
Authors: Engler Leonardo G. , Giustina Marina Della , Giovanela Marcelo , Roesch‐Ely Mariana , Gately Noel , Major Ian , Crespo Janaina S. , Devine Declan M. 
Published: January 2025

ABSTRACTThis study provides a comprehensive investigation of antimicrobial additives (ZnO/AgNPs and SiO2/AgNPs) on the properties of biodegradable ternary blends composed of poly(hydroxybutyrate) (PHB), poly(lactic acid) (PLA), and polycaprolactone (PCL) by examining the morphology, thermal stability, crystallinity index, and cell viability of these blends. Overall, transmission electron microscopy (TEM) analysis revealed that AgNPs and SiO2 exhibited comparable sizes, whereas ZnO was significantly larger, which influences their release profiles and interactions with the blends. The addition of antimicrobials influences the rheology of the blends, acting as compatibilizers by reducing the intermolecular forces between biopolymers. Scanning electron microscopy (SEM) analysis revealed a matrix–core–shell structure, indicating enhanced interfacial interaction among the immiscible biopolymers, as predicted by their spreading coefficient. From thermal evaluations, PCL promotes overall thermal stability, where T5 (the temperature at which the sample loses 5% of its weight through thermal degradation) was more than 22% higher than T5 of blends, and the antimicrobials investigated tend to act as barriers to heat penetration, thereby influencing the degradation mechanism of the blends. Additionally, antimicrobials tend to increase material crystallinity, suggesting their nucleating effect. Both PLA and PCL have shown high viability for cell growth and proliferation. The 30/50/20 (PHB/PLA/PCL wt%) blends were conducive to cell adhesion and proliferation, achieving cell viability rates up to 85% irrespective of the antimicrobial concentration. SEM analysis also confirmed the presence of viable cells and attachment of organic cell structures over the surface of the produced materials. In conclusion, this study highlights the potential of biodegradable ternary blends containing antimicrobial NPs, particularly for use in medical devices such as ureteral stents.
Gengnianchun Against H2O2‐Induced Oxidative Damage in KGN Cells via miR‐548m/FOXO3 Signaling
Authors: Li Jun , Rao Yanqiu , Pu Tao , Xu Ting , Wang Wenjun 
Published: January 2025

ABSTRACTGengnianchun (GNC) is a traditional remedy used for diminished ovarian reserve, but its underlying mechanisms remain unclear. This study aimed to explore these mechanisms in human granulosa‐like cancer (KGN) cells pretreated with medicated rat serum (MRS) before H2O2 exposure. MRS pretreatment significantly alleviated H2O2‐induced cell damage, including improvements in cell viability, superoxide dismutase and GSH‐Px activities, and Bcl‐2 expression. Conversely, H2O2 treatment increased apoptosis, autophagosomes, IL‐1β, TNF‐α, reactive oxygen species, malondialdehyde levels, and the expression of LC‐II/LC3‐I, Bax, and Beclin‐1. GEO database analysis revealed significant differential expression of several miRNAs, including miR‐548m. qPCR confirmed that MRS upregulated miR‐548m expression, which was downregulated by H2O2 in a dose‐dependent manner. Preincubation with MRS prevented the decline in miR‐548m expression and mitigated H2O2‐induced damage, including improvements in cell viability, apoptosis, autophagy, and oxidative stress. miR‐548m suppressed FOXO3 3'‐UTR luciferase activity, and anti‐miR‐548m enhanced it. Transfection with miR‐548m reduced FOXO3 mRNA and protein levels, while anti‐miR‐548m increased them. These findings suggest that GNC protects against H2O2‐induced ovarian damage by modulating the miR‐548m/FOXO3 axis, triggering autophagy and apoptosis.
The Impact of Circadian Preferences on Quality of Life in Patients With Gastroesophageal Reflux Disease: An Evaluation Using the SF‐36
Authors: Ergün Uğur , Güleç Ahmet , Tan Taner Buğra 
Published: January 2025

ABSTRACTBackground/AimsGastroesophageal reflux disease (GERD) is a common gastrointestinal disorder that negatively impacts individuals' quality of life.MethodsThis study investigates the effect of circadian preferences on the quality of life in patients with GERD. A total of 152 participants (80 patients diagnosed with GERD and 72 healthy controls) were included in the study. Participants' circadian preferences were assessed using the morningness‐eveningness questionnaire (MEQ), quality of life was evaluated with the SF‐36 scale, and the severity of GERD symptoms was measured using the gastroesophageal reflux disease quality of life scale (GERD‐QOL).ResultsThe study's findings indicate that the quality of life and circadian preferences of GERD patients differ significantly from those of the control group. Correlation analyses revealed a positive relationship between SF‐36 and MEQ, but no direct relationship was found between GERD‐QOL and SF‐36 or MEQ. Mediation analyses demonstrated that the effect of GERD‐QOL on SF‐36 is entirely mediated by circadian preferences.ConclusionsThese results highlight the significant impact of circadian preferences on the quality of life in GERD patients, suggesting that individuals with an evening chronotype may have lower quality of life. Our study contributes to the literature as one of the first to suggest that circadian preferences should be considered in the management of GERD.
Interaction Between YTH Domain‐Containing Family Protein 2 and SET Domain‐Containing Lysine Methyltransferase 7 Suppresses Autophagy in Osteoarthritis Chondrocytes, Exacerbating Cartilage Damage
Authors: Li Lexiang , Zhu Jun , Chen Yi , Li Haobo , Han Yaguang , Zhang Lei , Wang Bo 
Published: January 2025

ABSTRACTBackground and ObjectiveOsteoarthritis (OA) is characterized by progressive cartilage degeneration mediated by various molecular pathways, including inflammatory and autophagic processes. SET domain‐containing lysine methyltransferase 7 (SETD7), a methyltransferase, has been implicated in OA pathology. This study investigates the expression pattern of SETD7 in OA and its role in promoting interleukin‐1 beta (IL‐1β)‐induced chondrocyte injury through modulation of autophagy and inflammation.MethodsThe expression of SETD7 in cartilage tissues from OA patients and healthy controls was quantified using quantitative reverse transcription PCR and Western blot analysis. Small interfering RNA targeting SETD7 (si‐SETD7) was transfected into human articular chondrocytes (HACs) treated with IL‐1β to examine its impact on cellular viability, apoptosis, inflammatory responses, and autophagy. Functional assays including Cell Counting Kit‐8, flow cytometry, enzyme‐linked immunosorbent assay, and commercial kits were employed to assess biochemical changes. Interaction between YTH N6‐methyladenosine RNA binding protein 2 (YTHDF2) and SETD7 was explored using RNA immunoprecipitation and co‐immunoprecipitation assays.ResultsSETD7 was overexpressed in OA cartilage compared with controls and increased further upon IL‐1β treatment. Knockdown of SETD7 in IL‐1β‐treated HACs improved cellular viability, decreased apoptosis, and reversed the adverse effects on lactate dehydrogenase release and inflammatory markers (tumor necrosis factor‐alpha and interleukin‐6) while enhancing antioxidant enzymes (catalase, malondialdehyde, and superoxide dismutase). Additionally, autophagy was restored, as evidenced by changes in the levels of autophagy related 5, Beclin1, and sequestosome 1. Interfering with autophagy using chloroquine negated the protective effects of SETD7 knockdown. Furthermore, YTHDF2 was found to stabilize SETD7 mRNA, influencing its expression and enhancing IL‐1β‐induced chondrocyte injury.ConclusionSETD7 plays a critical role in the pathogenesis of OA by modulating chondrocyte survival, apoptosis, inflammation, and autophagy. The interaction between YTHDF2 and SETD7 exacerbates chondrocyte injury under inflammatory conditions, highlighting potential therapeutic targets for OA treatment. The YTHDF2/SETD7 axis offers a novel insight into the molecular mechanisms governing cartilage degeneration in OA.
Evaluation of Tumor‐Infiltrating Leukocytes in Endolymphatic Sac Tumor
Authors: Zhou Pei , Shi Xunbei , Ma Mengye , Wu Qianru , Wu Kunpeng , Li Feitian , Dai Chunfu 
Published: January 2025

ObjectiveEndolymphatic sac tumors (ELSTs), as rare low‐grade neoplasms, are primarily treated with surgery. This study analyzes the characteristics of tumor‐infiltrating leukocytes (TILs) in ELSTs and their relationships with clinical features to explore the potential for immunotherapy in ELSTs.MethodsClinical data and tumor specimens of 10 ELSTs patients who underwent surgery were retrieved. Immune expression levels of CD3, CD4, CD8, and CD66b were assessed by immunohistochemical staining and expressed as the integral optical density (IOD). Hematoxylin and eosin (HE) staining were performed to define the tumor nest and stroma of ELSTs.ResultsThere were no significant differences in the IOD of CD3, CD4, CD8, and CD66b between the tumor nest and stroma or between von Hippel–Lindau (VHL) and non‐VHL patients. The IOD of CD3, CD4, CD8, and CD66b appeared relatively higher in patients with endolymphatic hydrops (EH) than those without. Additionally, CD4 expression in the tumor stroma was significantly higher in patients with EH (p = 0.0381).ConclusionTILs were present in both ELSTs nest and stroma with significant individual heterogeneity observed among patients. Patients with VHL disease showed a similar immune pattern to those with sporadic ELSTs. Notably, the ELST‐related EH may be associated with lymphocytes infiltration. Combined with surgical intervention, immunotherapy may serve as an effective adjuvant therapeutic strategy.Level of Evidence4 Laryngoscope, 2025
Speckle‐Free 3D Holography in the Wigner Domain
Authors: Pi Dapu , Ye Yiqi , Cheng Ke , Gu Min , Fang Xinyuan 
Published: January 2025

AbstractComputer‐generated hologram (CGH) provides an approach to modulate the 3D coherent wavefront and has been widely used in many optical applications. Over the past few decades, extensive efforts have been made to design optimization models for high‐quality reconstruction. However, the reconstruction quality is still limited due to the mismatch of the bandwidth between the reconstructed field of interest and the reconstructed complex amplitude field in 3D space. Here, an ideal numerical light field mapping physical model from the hologram plane to the 3D image plane for speckle‐free 3D holography in the Wigner domain is established. With the aid of the Wigner distribution function (WDF), which allows the analysis of a light field from not only the space domain but also the spatial frequency domain, the bandwidth properties of the reconstructed field in 3D space are analyzed, which provides a guideline for the sampling of the reconstructed field to efficiently describe the speckles and artifacts. Accordingly, a comprehensive CGH optimization method in the Wigner domain is proposed to constrain the reconstructed intensity fluctuations without errors and omissions for high‐quality reconstruction. As such, this method enables speckle‐free and artifact‐free reconstruction with a twice improvement in peak signal‐to‐noise ratio (PSNR) and a five times improvement in structural similarity index measure (SSIM) compared to conventional phase‐only holograms. The optical experimental results show that this method paves the road for the future implementation of speckle‐free color 3D holography harnessing the advanced integrated photonic devices, and also offers an efficient and practical route for various optical applications, such as 3D display, optical encryption, beam shaping, optical computing and so on.
RNA modifications in cancer
Authors: Wu Han , Chen Shi , Li Xiang , Li Yuyang , Shi He , Qing Yiwen , Shi Bohe , Tang Yifei , Yan Zhuoyi , Hao Yang , Wang Dongxu , Liu Weiwei 
Published: January 2025

AbstractRNA modifications are emerging as critical cancer regulators that influence tumorigenesis and progression. Key modifications, such as N6‐methyladenosine (m6A) and 5‐methylcytosine (m5C), are implicated in various cellular processes. These modifications are regulated by proteins that write, erase, and read RNA and modulate RNA stability, splicing, translation, and degradation. Recent studies have highlighted their roles in metabolic reprogramming, signaling pathways, and cell cycle control, which are essential for tumor proliferation and survival. Despite these scientific advances, the precise mechanisms by which RNA modifications affect cancer remain inadequately understood. This review comprehensively examines the role RNA modifications play in cancer proliferation, metastasis, and programmed cell death, including apoptosis, autophagy, and ferroptosis. It explores their effects on epithelial–mesenchymal transition (EMT) and the immune microenvironment, particularly in cancer metastasis. Furthermore, RNA modifications’ potential in cancer therapies, including conventional treatments, immunotherapy, and targeted therapies, is discussed. By addressing these aspects, this review aims to bridge current research gaps and underscore the therapeutic potential of targeting RNA modifications to improve cancer treatment strategies and patient outcomes.
The Impact of the COVID‐19 Pandemic on the Well‐Being of People With Borderline Personality Disorder: A National Australian Study
Authors: Heidari Parvaneh , Broadbear Jillian H. , Mohammadshahi Marita , Foroughinia Farzaneh , Brown Rita , Rao Sathya 
Published: January 2025

ABSTRACTSocial isolation due to the COVID‐19 pandemic was a significantly destabilizing factor for people with borderline personality disorder (BPD). The aim of this study was to investigate the well‐being of Australians diagnosed with BPD during the initial lockdown to restrict the spread of COVID‐19. An online survey asked Australian residents (≥ 18 years) who self‐identified with having a diagnosis of BPD, about their experience of “coronaphobia” using the Coronavirus Anxiety Scale (CAS) as well as using open‐ and close‐ended questions to measure demographic and health information. The CAS data were analyzed descriptively. The World Health Organisation definition of health was used for deductive thematic analysis of qualitative data. Thirty‐five (24.5%) of the 143 participants who completed the CAS, experienced dysfunctional COVID‐19 related anxiety. Nearly half (n = 76; 46.1%) reported that their mental health became a lot worse. Utilizing the WHO definition of health, two main themes were identified: (1) Physical Health, and (2) Mental and Social Well‐being. Both themes included three subthemes that were variously affected by the national lockdown. Following the imposition of social restrictions to control the spread of the coronavirus, many people living with BPD experienced heightened challenges and greater vulnerability to adverse outcomes attributable to their underlying mental health symptoms.
Elevated Risk of Thyroid Malignancy in Biological Males Taking Estrogen Hormone Therapy
Authors: Meci Andrew , Lorenz F. Jeffrey , Goyal Neerav , Goldenberg David 
Published: January 2025

AbstractObjectiveThe role of estrogen in developing thyroid malignancy is poorly understood. Epidemiological studies have shown exogenous estrogen is associated with increased risk in females. Still, no studies to date have investigated this association among biological males undergoing estrogen hormone therapy.Study DesignRetrospective cohort study.SettingTriNetX Research Network.MethodsBiologically male patients prescribed estrogen with at least 5 years of follow‐up were queried from the database. Rates of diagnosis of malignant neoplasm of the thyroid gland within 5 years of estrogen hormone therapy prescription were determined, and statistics were conducted within the TriNetX platform.ResultsWe identified 6394 biologically male patients from 65 health care organizations prescribed estrogen hormone treatment. The average age was 44.4 years. When balanced for demographic factors as well as known risk factors for thyroid malignancy, the estrogen treatment cohort had a 0.64% risk for diagnosis of thyroid malignancy within 5 years, compared to a 0.27% risk among patients not taking estrogen (relative risk: 2.35, 95% confidence interval: 1.34‐4.15, P = .002).ConclusionWe found a higher risk of developing thyroid cancer in biologically male patients prescribed estrogen hormone therapy. This is the first association found between estrogen and thyroid malignancy in this group.
Mechanism Insights Into High‐Performance Cu2+ Adsorption by ZIF‐8/PAN Nanocomposite Fibers: Coordination and Ion Exchange Dynamics
Authors: Zhang Hailong , Li Ruimeng , Xu Haishui , Quan Ling , Zhan Chengyu , Han Peng , Xu Kehan , Tong Yuping 
Published: January 2025

ABSTRACTThe accumulation of copper ions in the environment can devastate the ecological systems and threaten the health of organisms. Consequently, the development of effective adsorbents to extract Cu2+ from aqueous solutions is urgently needed. Here, the Zeolitic Imidazolate Framework‐8 (ZIF‐8)/polyacrylonitrile (PAN) nanocomposite fibers were synthesized by electrospinning and further characterized using X‐ray diffraction (XRD), scanning electron microscopy (SEM), and Fourier transform infrared spectroscopy (FTIR). ZIF‐8 powders were uniformly distributed on the surface of the nanocomposite fibers, and amide CONH bonds were formed between the NH2 groups of ZIF‐8 and the CO groups of PAN. The ZIF‐8/PAN nanocomposite fibers demonstrated an exceptional Cu2+ adsorption capacity of 275.7 mg g−1 at pH = 4.0. The adsorption process adhered to the pseudo‐second‐order kinetics and the Langmuir isotherm model. The adsorption mechanism, as conformed by FTIR and X‐ray photoelectron spectroscopy (XPS) analysis, involves the coordination of the NH2 and CO groups with Cu2+ and the ion exchange processes between Cu2+ and Zn2+ for the nanocomposite fibers. This study provides a novel approach for the removal of Cu2+ from aqueous solutions and addresses the challenge of recovering powder materials in wastewater treatment.
MXene‐assisted click chemistry of L‐cysteine for the preparation of highly efficient damping materials
Authors: Peng Shuoli , Guo Liyun , Geng Feiyang , Liu Yudong , Liu Jinhui , Hua Jing 
Published: January 2025

AbstractL‐cysteine, a bio‐based modifier with multiple functional groups (thiol, amino, and carboxyl), holds promise for polymer modification. However, its tendency to agglomerate and poor solubility in nonpolar polymers limits its effectiveness. In this study, 1,2‐polybutadiene (1,2‐PB) was intercalated within MXene layers to enhance L‐cysteine dispersibility. The intercalation allowed nanoscale distribution of 1,2‐PB within MXene sheets, while interactions between MXene's hydroxyl groups and L‐cysteine's polar groups promoted uniform dispersion in the rubber matrix. Compared with physical blending, the grafting degree of L‐cysteine is higher, thereby more effectively restricting the movement of molecular chains, which leads to an increase in the effective damping temperature by over 7°C. Additionally, due to the ionic interactions between the functional groups, the resulting composite exhibits favorable dielectric performance, with the dielectric constant increasing from 2.5 to 3.2. Experimental results demonstrate that the synergistic effect between MXene and L‐cysteine improves both the damping and dielectric properties of the resulting composites. This study presents a novel approach using two‐dimensional nanosheets and bio‐based thiols for modifying polybutadiene, offering a potential path for high‐performance functional materials.Highlights
In‐situ polymerization enhances MXene distribution in the matrix.
Grafting degree of L‐cysteine increases with MXene incorporation.
Well‐dispersed MXene reduces L‐cysteine aggregation, enhancing mechanical strength.
Effective damping temperature rises by over 7°C with MXene and L‐cysteine.
Dielectric constant of composites increases from 2.5 to 3.2.
Circular economy: A sustainable model for waste reduction and wealth creation in the textile supply chain
Authors: Das Anik Kumar , Hossain Md. Farhad , Khan Borhan Uddin , Rahman Md. Mahfuzur , Asad M. A. Z. , Akter Mahmuda 
Published: January 2025

AbstractThe textile industry has long been a cornerstone of the global economy, but its environmental impact has come under scrutiny, particularly with the rise of fast fashion driven by rapid population growth and short‐term trends. The industry's current linear economy model, which prioritizes constant production and disposal, exacerbates its environmental footprint. To address these issues, a circular economy (CE) model has emerged, emphasizing the principles of “reduce, reuse, and recycle” to extend product life cycles, transform waste into wealth such as recycled yarn, regenerated fibers, biofilms, biodegradable composites, biofuels and so on, and minimize environmental impact. Implementation of CE in the textile industry aligns with the UN's sustainable development goals, as CE aims to minimize water, resources, and energy consumption, accelerate economic growth, and promote responsible consumption and production. This article explores how transitioning to a CE model could mitigate the industry's environmental damage while influencing economic growth. Furthermore, how internet of things (IoT) (Internet of things) can be contributed to CE initiatives by providing a transparent traceability along the whole textile supply chain. The review begins by examining the drawbacks of the linear economy and then highlights the environmental and economic benefits of CE. The discussion includes waste management strategies, with a special focus on recycling techniques not only in the end products but also across various process sections of the textile industry, promoting a sustainable development and application of recycled fiber. Additionally, it explores approaches like resale, reuse, repurposing, and rental. Furthermore, the paper addresses the economic and environmental benefits from circular economy principles adaptation supported by industry‐specific data. This paper shows the connection of sustainable development goals (SDGs) with circular economy, effects of consumer behavior in CE and potential risks associated with adopting a circular economy model, which are crucial for its successful implementation.Highlights
The linear textile economy worsens environmental impact through wasteful production.
Circular economy extends product life, transforming waste into valuable resources.
CE supports UN SDGs by reducing water, energy, and promoting responsible production.
IoT aids circular economy by ensuring transparent supply chain traceability in textiles.
The review focuses on CE's benefits, emphasizing recycling and sustainable waste management.