bluuebunny/crossref_metadata_2025_split huggingface dataset:
Host starvation andin hospitedegradation of algal symbionts shape the heat stress response of theCassiopea-Symbiodiniaceae symbiosis
Authors: Toullec Gaëlle , Rädecker Nils , Pogoreutz Claudia , Banc-Prandi Guilhem , Escrig Stéphane , Genoud Christel , Olmos Cristina Martin , Spangenberg Jorge , Meibom Anders 
Published: June 2023

AbstractGlobal warming is causing large-scale disruption of cnidarian-Symbiodiniaceae symbioses fundamental to major marine ecosystems, such as coral reefs. However, the mechanisms by which heat stress perturbs these symbiotic partnerships remain poorly understood. In this context, the upside-down jellyfishCassiopeahas emerged as a powerful experimental model system. We combined a controlled heat stress experiment with isotope labeling and correlative SEM–NanoSIMS imaging to show that host starvation is a central component in the chain of events that ultimately leads to the collapse of theCassiopeaholobiont. Heat stress caused an increase in catabolic activity and a depletion of carbon reserves in the unfed host, concurrent with a reduction in the supply of photosynthates from its algal symbionts. This state of host starvation was accompanied by pronouncedin hospitedegradation of algal symbionts, which may be a distinct feature of the heat stress response ofCassiopea. Interestingly, this loss of symbionts by degradation was to a large extent concealed by body shrinkage of the starving animals, resulting in what could be referred to as ’invisible’ bleaching. Overall, our study highlights the importance of the nutritional status in the heat stress response of theCassiopeaholobiont. Compared with other symbiotic cnidarians, the large mesoglea ofCassiopea, with its structural sugar and protein content, may constitute an energy reservoir capable of delaying starvation. It seems plausible that this anatomical feature at least partly contributes to the relatively high stress tolerance of these animals in our warming oceans.
Ubiquitin ligase and signalling hub MYCBP2 is required for efficient EPHB2 tyrosine kinase receptor function
Authors: Chang Chao , Banerjee Sara L. , Park Sung Soon , Zhang Xiaolei , Cotnoir-White David , Opperman Karla J. , Desbois Muriel , Grill Brock , Kania Artur 
Published: June 2023

AbstractEph receptor tyrosine kinases participate in a variety of normal and pathogenic processes during development and throughout adulthood. This versatility is likely facilitated by the ability of Eph receptors to signal through diverse cellular signalling pathways: primarily by controlling cytoskeletal dynamics, but also by regulating cellular growth, proliferation, and survival. Despite many proteins linked to these signalling pathways interacting with Eph receptors, the specific mechanisms behind such links and their coordination remain to be elucidated. In a proteomics screen for novel EPHB2 multi-effector proteins, we identified human MYC binding protein 2 (MYCBP2 or PAM or Phr1). MYCBP2 is a large signalling hub involved in diverse processes such as neuronal connectivity, synaptic growth, cell division, neuronal survival, and protein ubiquitination. Our biochemical experiments demonstrate that the formation of a complex containing EPHB2 and MYCBP2 is facilitated by FBXO45, a protein known to select substrates for MYCBP2 ubiquitin ligase activity. Formation of the MYCBP2-EPHB2 complex does not require EPHB2 tyrosine kinase activity and is destabilised by binding of ephrin-B ligands, suggesting that the MYCBP2-EPHB2 association is a prelude to EPHB2 signalling. Paradoxically, the loss of MYCBP2 results in increased ubiquitination of EPHB2 and a decrease of its protein levels suggesting that MYCBP2 stabilises EPHB2. Commensurate with this effect, our cellular experiments reveal that MYCBP2 is essential for efficient EPHB2 signalling responses in cell lines and primary neurons. Finally, our genetic studies inC. elegansprovidein vivoevidence that the ephrin receptor VAB-1 displays genetic interactions with known MYCBP2 binding proteins. Together, our results align with the similarity of neurodevelopmental phenotypes caused by MYCBP2 and EPHB2 loss of function, and couple EPHB2 to a signaling effector that controls diverse cellular functions.
GpsB control of PASTA kinase activity inListeria monocytogenesinfluences peptidoglycan synthesis during cell wall stress and cytosolic survival
Authors: Kelliher Jessica L. , Daanen McKenzie E. , Sauer John-Demian 
Published: June 2023

ABSTRACTThe ability to respond quickly to changing environmental conditions in the host is critical for bacterial pathogens. Penicillin-binding protein and serine/threonine-associated (PASTA) kinases are a conserved family of kinases important for cell envelope stress responses inFirmicutesandActinobacteria, including the cytosolic pathogenListeria monocytogenes. As serine/threonine kinases, PASTA kinases phosphorylate multiple substrates, yet the mechanisms through which these substrates promote resistance to cell wall stress remain poorly understood. We previously identified GpsB as a target of PrkA, the PASTA kinase inL. monocytogenes, through a phosphoproteomics screen. Here, we demonstrate that GpsB can be directly phosphorylated by PrkA, and that mutation of the PrkA-dependent phosphosite T88 to a phosphoablative residue enhances PrkA activityin vitro. We find that relative to a strain ofL. monocytogenesharboring thegpsBT88Aallele, a strain with the phosphomimeticgpsBT88Dallele is more sensitive to the cephalosporin antibiotic ceftriaxone and has a diminished capacity to increase peptidoglycan synthesis during stress. We find that GpsB-dependent control of PrkA activity is required for optimal survival and replication ofL. monocytogenesin the macrophage cytosol. Finally, we show that GpsB is required for full virulence ofL. monocytogenes, due in part to its role in modulating PrkA activity. Cumulatively, these results demonstrate that phosphorylative feedback between GpsB and PrkA is important for the ability ofL. monocytogenesto respond to cell wall stress, survive in its cytosolic niche, and cause infection.
A fitness landscape instability governs the morphological diversity of tip-growing cells
Authors: Ohairwe Maxim E. , Živanović Branka D. , Rojas Enrique R. 
Published: June 2023

SummaryCellular morphology affects many aspects of cellular and organismal physiology. This makes it challenging to understand the evolutionary basis for specific morphologies since the various facets of cellular physiology may exert competing selective pressures on this trait. The influence of these pressures, moreover, will depend on the mechanisms of cellular morphogenesis. To address this problem, we combined experimental cell biology with mechanics-based theory to analyze the morphological diversity of tip-growing cells from across the tree of life. We discovered that an instability in the convergent mechanism of “inflationary” growth shared by these cells leads directly to a bifurcation in their fitness landscape, which imposes a strong global constraint on their morphologies. Additionally, we found that co-selection for cell size and elongation rate explains variation among observable morphologies. This analysis rationalizes the morphology - and provides quantitative insight into the ecology - of an enormous diversity of important fungal, plant, protistan, and bacterial systems. Additionally, our study elucidates a fundamental principle of evolutionary-developmental biology that would be difficult to rigorously demonstrate in more complex systems.
Timing of Complementary Feeding for Early Childhood Allergy Prevention: An Overview of Systematic Reviews
Authors: Kuper Paula , Hasenpusch Claudia , Proebstl Simone , Matterne Uwe , Hornung Catherine J. , Grätsch Esther , Li Mengtong , Sprenger Antonia A. , Pieper Dawid , Koplin Jennifer J. , Perkin Michael R. , Genuneit Jon , Apfelbacher Christian 
Published: June 2023

AbstractObjectiveTo summarise and critically appraise systematic review (SR) evidence on the effects of timing of complementary feeding (CF) on the occurrence of allergic sensitisation and disease.DesignOverview of SRs. AMSTAR-2 and ROBIS were used to assess methodological quality and risk of bias (RoB) of SRs. RoB Tool 2.0 was used to assess RoB of primary randomised controlled trials (RCTs) (or extracted). The Certainty of Evidence (CoE) was assessed using GRADE. Findings were synthesised narratively.Data sourcesMEDLINE (via PubMed and Ovid), the Cochrane Library and Web of Science Core Collection.Eligibility criteriaSRs investigating the effects of timing of CF on risk of developing food allergy (FA), allergic sensitisation, asthma, allergic rhinitis, atopic eczema, and adverse events in infants or young children (0-3 years), based on RCT evidence.ResultsEleven SRs were included, with predominantly low methodological quality and high RoB. Primary study overlap was very high for specific FA and slight to moderate for FA in general and other primary outcomes. Introducing specific foods (peanut, cooked egg) early probably reduces the risk of specific FA based on evidence across most SRs. The evidence for other allergic outcomes was mostly very uncertain and based on single primary studies. SRs varied regarding the timing of CF, the nature of complementary foods and the population risk, which limited comparability between SRs.ConclusionsThe overlap of primary studies within SRs was high to very high for many outcomes, overemphasising single trials. Future research should focus on producing high quality trials and SRs that allow drawing more trustworthy conclusions. For developing guidelines to support decision-making on the timing of CF as a preventive strategy, the early introduction of specific foods (i.e., egg and peanut) seems promising and safe whereas more extensive research is required regarding other allergic outcomes and potential adverse events.RegistrationPROSPERO (CRD42021240160); Open Science Forum (https://doi.org/10.17605/OSF.IO/HJKUN)Key messagesEvidence supports early introduction of specific complementary foods (peanut, cooked egg) for preventing food-specific allergiesEvidence regarding prevention of other allergic diseases is sparse and of low certaintyFuture research should focus on producing high-quality trials and reviews for higher certainty of results
How can we compare CI systems across manufacturers? A scoping review of recent literature
Authors: Tzvi-Minker Elinor , Keck Andreas 
Published: June 2023

AbstractElectric stimulation via a Cochlear Implant (CI) enables people with severe to profound sensorineural hearing loss to regain speech understanding and music appreciation and thus allowing them to actively engage in social life. Three main manufacturers (Cochlear, MED-EL and Advanced Bionics “AB”) have been offering CI systems, thus challenging CI recipients and Otolaryngologists with a difficult decision, as currently no comprehensive overview or meta-analyses on performance outcome following CI implantation is available. The main goal of this scoping review is to provide evidence that data and standardized speech and music performance tests are available for performing such comparisons. To this end, a literature search was conducted to find studies that address speech and music outcomes in CI recipients. From a total of 1592 papers, 188 paper abstracts were analyzed and 147 articles were found suitable for examination of full text. From which, 42 studies were included for synthesis. A total of 16 studies used the consonant-nucleus-consonant (CNC) word recognition test in quiet at 60db SPL. We found that aside from technical comparisons, only very few publications compare speech outcomes across manufacturers of CI systems. Evidence suggests though, that these data are available in large CI centers in Germany and US. Future studies should therefore leverage large data cohorts to perform such comparisons that could provide critical evaluation criteria and assist both CI recipients and Otolaryngologists to make informed performance-based decisions.
Mitochondria- and ER-associated actin are required for mitochondrial fusion
Authors: Gatti Priya , Schiavon Cara , Cicero Julien , Manor Uri , Germain Marc 
Published: June 2023

AbstractMitochondria play a crucial role in the regulation of cellular metabolism and signalling. Mitochondrial activity is modulated by the processes of mitochondrial fission and fusion, which are required to properly balance respiratory and metabolic functions, transfer material between mitochondria, and remove defective mitochondria. Mitochondrial fission occurs at sites of contact between the endoplasmic reticulum (ER) and mitochondria, and is dependent on the formation of actin filaments that drive mitochondrial constriction and the recruitment and activation of the dynamin-related GTPase fission protein DRP1. The requirement for mitochondria- and ER-associated actin filaments in mitochondrial fission remains unclear, and the role of actin in mitochondrial fusion remains entirely unexplored. Here we show that preventing the formation of actin filaments on either mitochondria or the ER disrupts both mitochondrial fission and fusion. We show that fusion but not fission is dependent on Arp2/3, whereas both fission and fusion are dependent on INF2 formin-dependent actin polymerization. We also show that mitochondria-associated actin marks fusion sites prior to the dynamin family GTPase fusion protein MFN2. Together, our work introduces a novel method for perturbing organelle-associated actin filaments, and demonstrates a previously unknown role for actin in mitochondrial fusion.
Uncovering the link between well-being and factory performance among workers in China: a longitudinal study
Authors: Bellingan Minette , Tilley Catherine , Kumar Mukesh , Batista Luciano , Evans Steve 
Published: June 2023

PurposeCompanies are concerned about the well-being of workers in their supply chains, but conventional audits fail to uncover critical problems. Yet, if the happy worker – productive worker thesis is correct, it would benefit factories in fast-developing countries, particularly China which is key to many global supply chains, to ensure the well-being of their workers. The authors set out to better understand the relationship between well-being and performance in four Chinese factories.Design/methodology/approachOver 12-months the authors collected digital diaries from 466 workers in four factories, and monthly data about the performance of their factories. The authors used this data to gain insights into the well-being of workers in these factories; to design experimental interventions to improve this; and to consider any effects these had on factory performance.FindingsThe experiments showed that training interventions to improve workers' well-being through their work relationships and individual skills improved not just a factory's general worker well-being, but also some aspects of its performance and worker retention. Thus, it brought benefits not only for the workers but also for the factory owners and their client companies.Originality/valueWhile there is a significant body of research investigating the happy worker – productive worker thesis, this was not conducted in Chinese factories. The authors’ work demonstrates that in this and similar environments, workers' eudaimonic well-being is more important than might be assumed, and that in this context there is a relationship between well-being and performance which can be practically addressed.
The effects of pre-scandal associations of athlete endorsers and scandal types on consumer blame and eWOM
Authors: Sato Shintaro , Ko Yong Jae , Kim Daehwan , Lee Joon Sung 
Published: June 2023

PurposeThe purpose of this research is to examine how pre-scandal associations and scandal types interactively influence consumer judgment and negative electronic word-of-mouth (eWOM).Design/methodology/approachDrawing from cognitive dissonance theory and associative memory network model, the online experiments (Amazon Mechanical Turk; Nexperiment 1 = 146 and Nexperiment 2 = 189) were conducted to examine the effects of positive pre-scandal associations (performance vs pro-social) and scandal types (performance-related vs -unrelated) on consumer blame and eWOM toward scandalized athletes. Data were analyzed by employingt-test (experiment 1), Multivariate Analysis of Covariance (MANCOVA) and PROCESS Model 8 (experiment 2) to test the hypotheses.FindingsThe findings highlight that positive pre-scandal association demonstrated both protecting and backfiring effects depending on the types of scandals. Specifically, when performance-related scandals emerged, consumers made more negative blame judgment of athletes with salient performance association, relative to pro-social association. Inversely, when performance-unrelated scandals occurred, athletes with salient pro-social association were more likely to be blamed. Regarding eWOM, consumers generate more negative eWOM when athletes with pre-performance associations are involved with performance-related scandals. This pattern of the result was not observed when athletes' pro-social association and performance-unrelated scandals were prominent.Originality/valueThe current work adds consumers' negative eWOM toward scandalized athletes to the literature as a predictor of how athletes' pre-scandal association with consumers and scandal types are related. The findings indicate that consumers feel greater dissonance and generate more negative eWOM when athletes' pre-scandal associations and scandal types are closely related.
Trends in E-learning research: bibliometric analysis on Scopus publications post COVID-19 in Asian context
Authors: Pathak Shipra , Singh Navjit 
Published: June 2023

PurposeThe purpose of this paper is to explore future directions in E-learning research by analysing data from Scopus indexed publications in order to have a comprehensive overview of the trends and thematic focus post COVID-19 in Asian context.Design/methodology/approachThe paper uses Vos viewer and Biblioshiny software packages to analyse the bibliometric data. This software helped in identifying the anatomy of E-learning and their themes which were instrumental in forecasting future trends.FindingsThe paper depicts the trends in post COVID-19 E-learning research in Asian context. It identifies key publications, authors and journals in the field, with a focus on numerous networks of collaboration between writers and nations, identifying keyword clusters and co-citation analysis clusters. This study also explored that China and the USA are having maximum number of collaborations, whereas, countries like India, the United Kingdom, Singapore and New Zealand have comparatively weaker collaboration networks. So there is lot of potential for these countries for such collaborations. India is the most cited country globally and China is having maximum number of scientific productions per year.Research limitations/implicationsThe paper has been written by exclusively referring to Scopus database papers. Collecting data from different databases would significantly improve the study. Future researchers can also focus on papers from psychology, computer science and engineering fields as current work is based on open access articles on social business, business and arts and humanities.Practical implicationsThis research will be useful to educational institutions that use these platforms to offer E-learning content and match future trends. This study will help researchers in understanding the new dimensions in the field of E-learning.Originality/valueThe current study uses bibliometric analysis to examine the association between E-learning, higher education and COVID-19. It aids in the identification of new difficulties within the complex and expanding study fields in the world of E-learning. Newly published studies on E-learning trends can improve understanding and bridge the knowledge gap. As a result, recommendations can be made to improve and implement newer strategies in field of education.
The responsibility of ensuring food security: a cross-country study on reducing the impact of agricultural industries on vulnerability to climate change
Authors: Galbreath Jeremy , Ljubownikow Grigorij , Tisch Daniel , Tuazon Gerson 
Published: June 2023

Purpose
Considering that food security is a global responsibility, the purpose of this study is to examine the impact of agricultural industries on vulnerability to climate change and the moderating effects of gender-diverse parliaments, education expenditures, research and development (R&D) expenditures and foreign direct investment (FDI).

Design/methodology/approach
Using concepts in governance, innovation and knowledge theory, a large panel data set of 125 countries covering 1997–2018 (1,852 country-year observations) was analyzed. Data were sourced from the Notre Dame Global Adaptation Index, the World Bank, the Heritage Index and the International Monetary Fund. Moderated random effects regression was conducted in Stata.

Findings
The results reveal that agricultural industries are positively associated with vulnerability to climate change and provide support for our predictions that education expenditures and FDI both reduce the impact of agricultural industries on vulnerability to climate change. However, contrary to predictions, the percentage of women in parliament and R&D expenditures both increase this impact.

Originality/value
To the best of the authors’ knowledge, this is the first quantitative study that uses large, established data sets to explore the relationship between agricultural industries and country vulnerability to climate change. This study shows the significance of country-level factors that both decrease and increase the impact of agricultural industries on vulnerability to climate change.
Determinants of satisfaction with online food delivery providers and their impact on restaurant brands
Authors: Macias Washington , Rodriguez Katia , Barriga Holger 
Published: June 2023

Purpose
This paper aims to analyze the spillover effects between online food delivery providers’ (OFDs) performance and restaurant brands. It proposed a model of three determinants for e-satisfaction with OFDs and related these variables to restaurants’ brand satisfaction, image and loyalty.

Design/methodology/approach
A survey was designed, and 332 responses from delivery apps users in Ecuador were collected. A partial least squares structural equations model was applied.

Findings
The three proposed determinants of OFD satisfaction were significant: e-service quality, personal aspects of delivery workers and perceived food quality. Regarding the spillover effects, e-service quality and personal aspects had an influence on perceived food quality, mediating a chain of effects on restaurants’ brand satisfaction, image and loyalty.

Research limitations/implications
Data were collected before COVID-19. Further studies will need to be undertaken in the context of the COVID-19 pandemic because minimal contact between delivery workers and customers is expected. In addition, food quality perceptions may include new concerns about biosafety norms.

Practical implications
Practices aiming to improve the service experience with OFDs are suggested, including proper training, supervision and improvement of delivery workers’ conditions. On the other hand, because restaurants do not control OFD’s performance, their contractual agreements should focus on avoiding service failures that erode restaurants’ brand equity.

Originality/value
This paper fulfills the need to study the spillover effects in the context of the collaborative economy, where delivery companies, delivery workers and restaurants work together to provide a service, and the performance of one of the parties impacts the consumers’ perceptions of the other party.