bluuebunny/crossref_metadata_2025_split huggingface dataset:
Phase transition study of Al-Si Co-deposited martensitic steel
Authors: Anwer Zubia , Riaz Muhammad , Bhatti Muhammad , Bhatti Jahanzeb , Shaikh Sajida 
Published: August 2023

Abstract
The oxidation performance at elevated temperatures for Al-Si coated Martensitic Stainless Steel AISI 410 was investigated. The pack aluminizing at 1050°C for 3 h was employed for the co-deposition of Al and Si onto the alloy. The microstructural performance and material’s response towards oxidation and corrosion behavior along with phase transition at 1100°C with various exposure periods have been observed. In particular, the Al2O3, Al2SiO5, Fe2SiO4, Cr2SiO4, etc. are the protective oxides, resulting from oxidation, decreasing the DoS. The Al2O3 and Al2SiO5 are observed as responsible for protective oxide phases. The highest mass gain was obtained during the period of 16 h to 20 h due to the formation of protective oxides. The increment in the degree of spallation has been observed for the oxidation period of 12h to 16 h. The hot corrosion resistance of the bared and the coated sample was observed in a salt-based environment and found satisfactory results for Al-Si coated alloy for a 12 h to 24 h exposure period.
Bone destruction of orbital wall: does it always imply malignancy?
Authors: Guo Qihan , Yang Bentao , Liu Rui , Wang Nan , Xu Liangyuan , Zhang Xuan , Ma Jianmin 
Published: August 2023

Abstract
This study reviewed the clinical characteristics, treatments, and outcomes of six patients (seven eyes) with idiopathic orbital inflammatory pseudotumor with bone destruction. CT and/or MRI showed that the medial wall was the most common site of orbital wall destruction (four of the seven eyes). All patients underwent surgery and were treated with postoperative methylprednisolone. Prognosis was assumed poor as this condition features by a wide range of anatomical involvement. The study suggests that complete surgical resection may lead to a favorable prognosis, as half of the patients reported no recurrence and a total relief of symptoms. We proposed a new classification of bone destruction for this condition based on imaging studies, which is usually perceived as malignant lesions. Histopathological examination for the definitive diagnosis of this condition is crucial.
Distribution and evolution of plasmodial infection in Madagascar according to incidence per 100,000 inhabitants
Authors: Randriananahirana Zina Antonio , Raherinandrasana Antso Hasina , Ratovondramamy Davy Alain , Ramotalambo Tantely Jenny , Aivotiana Andrianavony Ny , Rafamatanantsoa Jean Florent , Raobela Omega , Ravaoarisoa Lantonirina , Andrianasolo Radonirina Lazasoa , Rakotonirina Julio 
Published: August 2023

Abstract
Background
Malaria is still a public health problem in Madagascar. The infection remains difficult to eliminate. The objective of this study is to establish the spatio-temporal distribution of malaria in Madagascar by considering the incidence profile.
Methods
This is a cross-sectional study that identifies the temporal-spatial evolution of malaria. The study period extends from 2019 to 2021. The analysis of the trend of malaria is analyzed through the evolution of the incidence and the spatial distribution using a thematic cartography.
Results
The present study revealed that the trend of malaria infection increased from 2019 to 2021. Spatially, malaria infection still experiences a heterogeneous distribution according to the epidemiological facies. The study was also able to highlight the importance of reliable data for disease surveillance. Control strategies must prioritize the entire pediatric age group for prevention and treatment but not only those under 5 years of age.
Conclusion
the fight against malaria must take into account the seasonality of the infection and the distribution of resource necessary for the fight against malaria must consider the heterogeneity of the spatial distribution of the infection.
YAP activation and Hippo signaling suppression by PKC eta promote triple-negative breast cancer metastasis
Authors: Belsamma Liju Vijayasteltar , Muraleedharan Amitha , Jayaram Divya Ram , Waidha Kamran , Jagadeeshan Sankar , Sinay Rose , Eremenko Ekaterina , Berner Omer , Elkabets Moshe , Livneh Etta 
Published: August 2023

Abstract
Breast cancer is the leading cause of cancer-related deaths in women, with metastasis being the primary reason for mortality. Patients with triple-negative breast cancer (TNBC) show an increased risk of metastatic dissemination. Protein kinase C eta (PKCη), an anti-apoptotic kinase of the novel PKC subfamily, is associated with poor prognosis in breast cancer patients. Here, we demonstrate that PKCη promotes metastasis in TNBC cells and show that this is mediated by the PKCƞ-YAP signaling axis. Knockout of PKCη (PKCηKO) in the TNBC cells, 4T1 and MDA-MB-231, markedly inhibited their invasion and migration capability. Furthermore, orthotopic xenografts of the latter PKCηKO cells in NSG mice reduced tumor growth and lung metastasis compared to PKCη-intact tumors. Mechanistically, we show that PKCη regulates epithelial-to-mesenchymal transition (EMT), as knockout of PKCη in TNBC cell lines increased expression of the EMT markers E-cadherin, EpCAM, and slug, and decreased expression of vimentin, ZEB1. Further profiling of the Hippo-YAP axis showed that PKCη is a negative regulator of the Hippo pathway that leads to YAP stabilization and its phosphorylation at Ser128, which allows YAP to translocate to the nucleus and contribute to metastasis of TNBC cells. We further show that PKCη directly interacts with YAP in silico and TNBC cells. Lastly, we demonstrate that treatment of TNBC cells with uPEP2, a recently discovered PKCη kinase inhibitory peptide (encoded by a uORF upstream of PKCη coding sequence), activates the Hippo pathway and YAP degradation. In summary, our results highlight the impact of PKCη in TNBC metastasis and offer a novel avenue for therapeutic intervention in this aggressive and fatal disease.
Badminton Hawk-Eye Challenge: A Lightweight Badminton Detection Network Based on Feature Pyramid
Authors: Jin Hua , Qiang Zhiheng , Yan Jingrui , Zhang Xing , Wang Changda 
Published: August 2023

Abstract
The utilization of Hawk-eye technology is a reliable method to ensure the fairness and precise while playing. However, due to the high hardware cost such an application in amateur badminton matches is nearly impossible. To address this problem, this study proposes a convolutional neural network (CNN) based on the feature pyramid structure for badminton place’s detection. The model incorporates the improved MobileNetV3 structure with the CBAM mixed attention mechanism, the improved CSP structure designed to reduce redundant computation, and reach low memory access through redundant feature information. Its objective is that we aim to develop a lightweight model. Furthermore, we have incorporated the NWD loss function and frame difference to improve the accuracy of the model. Morphological processing is then employed to divide the outline of the badminton with the boundary line of the field and determine whether it is out of bounds. The model is experimentally evaluated on the badminton dataset created by us. The results indicate that the precise reaches 82.8$\%$, and the map0.5 reaches 76.8$\%$ of the compared YOLOv5 algorithm. Furthermore, the GFLOPs and parameter quantity of the proposed model are reduced to 34.9$\%$ and 50.46$\%$ of the compared YOLOv5 algorithm. Based on which we designed a challenge system for amateur badminton enthusiasts. Our lightweight Hawk-Eye challenge system has much lower hardware costs than the existing Hawk-Eye Challenge system, and is flexible in hardware deployment.
Alteration of apparent diffusion coefficient measurements predict survival outcomes during neoadjuvant chemoradiotherapy in locally advanced rectal cancer
Authors: Wada Yuma , Nishi Masaaki , Yoshikawa Kozo , Tokunaga Takuya , Nakao Toshihiro , Kashihara Hideya , Takasu Chie , Yoshimoto Toshiaki , Shimada Mitsuo 
Published: August 2023

Abstract
Background
Neoadjuvant chemoradiation therapy (nCRT) followed by surgery is recommended for patients with locally advanced rectal cancer (LARC). However, because 30–40% of patients with LARC do not respond to nCRT, better prediction of treatment responses and survival outcomes is required. Therefore, we aimed to identify apparent diffusion coefficient (ADC) values that predict survival outcomes after nCRT in patients with LARC.
Methods
We analyzed 66 patients with LARC who underwent nCRT and evaluated ADC values of pre- and post-nCRT. We performed Cox proportional hazard regression analyses for survival outcomes.
Results
There were no significant differences in disease-free survival (DFS) and overall survival (OS) between low and high ADC values of pre-nCRT. However, patients classified as low ADC in post-nCRT had a significantly worse prognosis in OS and DFS (OS: P = 0.01; DFS: P < 0.01) than patients classified as high ADC. Moreover, an alteration in ADC values between pre- and post-nCRT was associated with poor OS (univariate: hazard ratio [HR] = 4.52, 95% confidence interval [CI] = 1.58–12.97, P < 0.01; multivariate: HR = 6.38, 95% CI = 1.48–27.56, P = 0.01). Finally, we identified ADC values that were significantly superior for predicting tumor regression grade with remarkable diagnostic accuracy (post-nCRT: area under the curve [AUC] = 0.79; alteration-nCRT: AUC = 0.85).
Conclusions
We identified the clinical importance of alteration of ADC values to predict the survival outcomes in patients with LARC. These results highlight the clinical importance of ADC values on improving the treatment strategies of patients.
Effects of bio-fermented feed on growth, digestion, immunity, antioxidant capacity, and intestinal structure of juvenile yellow catfish (Pelteobagrus fulvidraco)
Authors: 周 泽湘 , Fang Liu , Zhao Xueru , Shu Jiu , Liu Yulin , Ruan Guoliang , Wang Qian , Lin Fawen , Liu Haijun 
Published: August 2023

Abstract
To investigate the feeding effect of fermented feed, juvenile yellow catfish (1.23±0.54) g were fed with hard pellet feed and biological fermented feed in an indoor recirculating water system for 8 weeks. The study analyzed the effects of fermented diets on the growth performance, digestion, immunity, antioxidant capacity, and intestinal structure of yellow catfish. The results showed that: the fermented diet significantly increasedthe WGR, SGR, and VSI, but had no significant effect on the HSI. The fermented diet significantly increased the activities of Try, Lip, and AMS in the intestinal tract of yellow catfish. The fermented diet significantly increased the activities of ACP, AKP, CAT, and SOD in the serum of yellow catfish and significantly decreased the content of MDA in the serum. The fermented diet significantly increased the width and height of the intestinal villus and the thickness of the intestinal wall in the midgut of yellow catfish.The results indicate that fermented feed could effectively improve the growth performance, digestive enzyme activity, and immune and antioxidant capacity, and improve the intestinal tissue structure of yellow catfish. This provides a theoretical basis for the production of microbial fermented feed.
M2 macrophage-derived exosomal long noncoding RNA Myt1l promotes lung metastasis of pancreatic cancer cells via the miR-135/Slug axis
Authors: wang Bin , Dang Shengchun 
Published: August 2023

Abstract
Purpose
Long non-coding RNAs (lncRNAs) in exosomes are important mediators of communication between tumor cells and their microenvironment. Previous studies have found that M2 macrophages promote the migration and invasion of pancreatic cancer (PC) cells through epithelial-mesenchymal transition (EMT). The aim of this study was to elucidate how M2 macrophage-derived exosomal lncRNAs promote lung metastasis of PC cells and provide new targets for future diagnosis and treatment of PC.
Methods
After verifying the promotion effect of M0 and M2 type macrophage exosomes on migration and invasion of pancreatic cancer cell line AsPC-1 by in vitro cellular experiments, we searched for the most significantly differentially expressed lncRNAs, predicted target genes, and detected the content of EMT-related proteins. Next, animal experimental models further examined the promotional effects of different levels of lncRNA and target genes on lung metastasis of AsPC-1 cells.
Results
Exosomes from M2 macrophages contain high levels of Myt1l, which acts as an endogenous sponge for miR-135 increasing the level of snail family transcriptional repressor 2 (Slug) in PC cells. The Myt1l-miR-135-Slug axis promotes metastasis of AsPC-1 cells to the lungs by increasing the expression of the EMT-associated protein vimentin.
Conclusions
Our studies identify a new mechanism by which Myt1l-miR-135-Slug regulates PC progression, supporting the possibility that PC patients may benefit from Myt1l as a new biomarker or therapeutic target.
Pseudomonas aeruginosa strains isolated from environments and patients analysed by cgMLST: The dominance of the emerging high-risk clone ST463 from intensive care unit in East China
Authors: Shi Weixiao , Zhang Piaopiao , Feng Haiting , Ge Tianxiang , Chen Yunbo , Jiang Yan , Yang Qing , Shen Ping , Ni Lingmei , Xiao Yonghong , Qu Tingting 
Published: August 2023

Abstract
Background: Carbapenem-resistant Pseudomonas aeruginosa sequence type 463 (ST463-CRPA) with high-virulence and high-resistance has emerged and spread rapidly in East China. The aim of the study was to investigate the distribution and characteristics of the emerging high-risk ST463-CRPA in intensive care unit (ICU) in East China.
Methods: The 11-month-span study investigated the epidemiological characteristics, genetic composition, and transmission pattern of CRPA isolated from patients and environments of 5 different ICUs in a large teaching hospital in East China. Whole-genome sequencing, antibiotic susceptibility testing and biofilm-forming ability testing were performed in all CRPA isolates. The molecular epidemiological characteristics and the phylogenetic relationships of isolates from different sources were assessed using multi-locus sequence typing (MLST) and core genome multi-locus sequence typing (cgMLST). Bacterial virulence was assessed using a Galleria mellonella infection model.
Results: According to the MLST scheme, all of CRPA isolates fell into 25 defined STs, of which 24.07% (13/54) CRPA belonged to ST463 as the dominant ST in ICUs. All 13 ST463-CRPA strains, including 4 from patients and 9 from environments mainly in washbasins, carried blaKPC-2 gene with exoU+/exoS+ virulence genotype showing difficult-to-treat resistance (DTR) phenotype. ST463-CRPA had higher biofilm-forming ability and mortality of infected larvae than non-ST463-CRPA (P<0.05). No differences on virulence were detected between the CRPA from environments and infected patients. CgMLST results revealed that several ST463-CRPA strains isolated from different ICUs had genetic relationship. Notably, 3 ST463 strains harboring both blaAFM-1 and blaKPC-2 genes with 41-59 SNPs difference were isolated from different ICUs.
Conclusions: In combination with epidemiological data linked in time and space, the cgMLST results elucidated polyclonal dissemination of CRPA and a distribution of ST463-CRPA among ICUs in a large hospital of East China. As the dominant ST found in ICU especially in environments, KPC-ST463-CRPA might be considered a strain with potential to cause a future outbreak, which could acquire the blaAFM gene additionally, leading to the resistance to last-resort antibiotics. Hence, it’s worth our vigilance about the growing prevalence of ST463-CRPA in ICU of East China. Meanwhile, it’s important to strengthen the management of ICU environment, especially the water reservoirs.
The role of FBXO32 in regulating the growth of esophageal cancer
Authors: Song Xian-qiang , Chen Bin-bin , Jin Yong-mei , Wang Chang-yong 
Published: August 2023

Abstract
Purpose
Esophageal cancer (EC) is a common and serious form of cancer. F-box protein 32 (FBXO32) is a member of the F-box protein family and its role in EC is still unclear.
Methods
FBXO32 expression was examined in EC cells using GSE163735 dataset and RT-qPCR and its effects on cell proliferation, migration, and invasion and epithelial mesenchymal transition (EMT) was investigated. The xenograft model established by injecting EC cells transfected with FBX032 was used to evaluate tumor cells growth, apoptosis, proliferation, and metastasis. ChIP assay was employed to study the interaction between FBXO32 with and DNA methyltransferase-1 (DNMT1). Finally, HitPredict, Co-IP, and GST pulldown assay was utilized to analyze the interaction between FBXO32 and CDK9.
Results
High FBXO32 expression was associated with better overall survival in patients. It is negatively regulated by DNMT1 in EC cells. DNMT1 bound to the FBXO32 promoter to promote its methylationand downregulation in EC cells. Knockdown of DNMT1 in these cells increased FBXO32 expression and suppressed malignant phenotypes. Mechanistically, FBXO32 ubiquitinated and degraded CDK9 (Cyclin Dependent Kinase 9) in EC cells which was prevented in FBXO32-silenced cells. Finally, EC cells overexpressed with FBXO32 inhibited tumor growth and metastasis in xenografts demonstrating its tumor suppressor role.
Conclusion
FBXO32 is a tumor suppressor that ubiquitinates and degrades CDK9 that results in inhibition of EC.
Early Mobilization in Pediatric Critical Care: Exploring the Gap between Theory and Practice in Saudi Arabia
Authors: Al-Harbi Samah 
Published: August 2023

Abstract

Background:Advancements in critical care have improved survival rates for children in PICUs, but many survivors suffer long-term morbidity and lower quality of life due to ICU-acquired weakness, cognitive impairment, and immobility-related issues. Early mobilization (EM) is a promising intervention to reduce these negative consequences. This study evaluates the knowledge, views, and practices of physicians and care providers regarding EM in Saudi Arabia's pediatric critical care units.
Study Design: A survey was conducted from July 2020 to February 2021 to gather information on early mobilization practices for critically ill children in the pediatric intensive care unit in Saudi Arabia. The survey was sent to 110 doctors, 200 nurses, 30 respiratory therapists, and 20 physiotherapists. The survey aimed to explore rehabilitation aspects, including the challenges faced during early mobilization, suitable timing and types of rehabilitation, and healthcare personnel workload.
Results:A survey with a 72.78% response rate, primarily from registered nurses (65.7%), indicated strong support for early mobilization in Pediatric Intensive Care Unit (PICU) patient care among healthcare professionals. Over 80% of respondents considered it crucial or important. However, institutional and healthcare provider-level barriers exist, including lack of equipment (63.5%), medical instability (67.3%), endotracheal intubation (65.4%), and limited staff (56.4%). Concerns about safety (47.4%) and insufficient physical space (38.9% physicians, 24.7% non-physicians) were also raised. Routine bed rest orders upon PICU admission were more common among non-physicians (26.8%) compared to physicians (12.5%). As illness severity increased, clinicians' comfort with early mobilization decreased. Most common rehabilitation interventions were respiratory physiotherapy and passive range of motion (77.8%). Healthcare professionals in Saudi Arabia regularly use daily sedation interruptions in pediatric ICUs. However, only 38.7% of respondents referred patients with suspected ICU-acquired weakness to outpatient clinics for long-term rehabilitation.
Conclusion: Early mobilization is crucial in pediatric critical care in Saudi Arabia, but its implementation faces challenges. Healthcare institutions must navigate these challenges with strategic intent and a multidisciplinary ethos. Future research should focus on optimal timelines and interventions tailored to specific pediatric cohorts. This approach can improve patient outcomes and elevate the caliber of pediatric critical care, refining healthcare quality and providing a blueprint for excellence globally.
Intersectional inequalities in the transition to grandparenthood and cognitive functioning: A longitudinal Multilevel Analysis of Individual Heterogeneity and Discriminatory Accuracy (MAIHDA)
Authors: Alonso-Perez Enrique , Heisig Jan Paul , Kreyenfeld Michaela , Gellert Paul , O'Sullivan Julie Lorraine 
Published: August 2023

Abstract

Objectives
With aging societies, more people become vulnerable to experiencing cognitive decline. While normal aging is associated with a deterioration in certain cognitive abilities, little is known about how social determinants intersect to create late-life cognitive functioning inequalities. Simultaneously, the role of grandparenthood is central for older adults and their families. There are indications that social determinants intersect to modulate the effect of the transition to grandparenthood, but further evidence is needed. Our study investigates the relation of transition to grandparenthood with cognitive functioning and explores differences across intersectional strata.
Methods
Using longitudinal data from the Survey of Health, Ageing and Retirement in Europe, we analyzed a sample of 19,953 individuals aged 50–85 without grandchildren at the baseline. We applied Multilevel Analysis of Individual Heterogeneity and Discriminatory Accuracy to investigate cognitive functioning differences across 48 intersectional strata, defined by sex/gender, migration, education, and occupation. We allowed the impact of becoming a grandparent to vary across strata by including random slopes.
Results
Intersectional strata accounted for 17.43% of the overall variance in cognitive functioning, with most of the stratum-level variation explained by additive effects of the stratum-defining characteristics. Transition to grandparenthood was associated with higher cognitive functioning, with a stronger effect for women. Stratum-level variation in the grandparenthood effect was modest.
Discussion
This study highlights the importance of social determinants for understanding heterogeneities in the association of transition to grandparenthood with cognitive functioning. Adopting an intersectional lens is useful to decompose inequalities and derive tailored interventions to promote equal healthy aging.
Roles of PCSK9 in the Pathogenesis of Periodontal Disease
Authors: Khosrojerdi Motahareh , Reiner Željko , Vatanparast Negin , Jamialahmadi Tannaz , Kesharwani Prashant , Sahebkar Amirhossein 
Published: August 2023

:
Proprotein convertase subtilisin/Kexin type 9 (PCSK9) inhibitors are FDA-approved
drugs recommended for high-risk patients with LDL-cholesterol (LDL-c) levels ≥
70 mg/dl. Several studies have also investigated the relationship between PCSK9 and periodontitis.
Specifically, studies have investigated the association between periodontitis
and periodontal PCSK9 levels in humans, and periodontium status in PCSK9-knockout
versus wild-type mice. While a positive association between periodontitis and periodontal
PCSK9 levels has been noted, the findings on the comparison of periodontium status
between PCSK9-knockout and wild-type mice have been inconsistent. Different methodologies
among these studies may explain this discrepancy. Future experimental studies
on the impact of pharmacological PCSK9 inhibition on periodontal status as well as observational
studies comparing periodontium status between patients receiving PCSK9 inhibitors
and those receiving other lipid-lowering drugs will shed light on the role of PCSK9
in periodontal health and disease.