bluuebunny/crossref_metadata_2025_split huggingface dataset:
Temporal and Spatial Analysis of Event-Related Potentials in Response to Color Saliency Differences Among Various Color Vision Types
Authors: Takahashi Naoko , Sawayama Masataka , Chen Xu , Motomura Yuki , Takeichi Hiroshige , Miyauchi Satoru , Hiramatsu Chihiro 
Published: September 2023

ABSTRACTIndividuals with minority color vision phenotypes have been reported to exhibit enhanced color discrimination and color recognition, which deviate from predictions based on their receptoral sensitivities. However, the specific mechanisms underlying this enhanced sensitivity remain unclear. In this study, we examined the commonality and diversity of neural activity between typical and anomalous trichromats in response to differences in color saliency. Electroencephalography was recorded during an oddball task, in which participants discriminated each of two target stimuli, blue-green and red, from a green standard stimulus. The chromaticity of the stimulus was identical across participants, whereas the relationship of saliency between the target stimuli was expected to be reversed between color vision types. The spatiotemporal dynamics of event-related potentials (ERPs) were analyzed using cluster-based permutation tests. Typical trichromats demonstrated faster behavioral and neural responses to the more salient red target stimulus, with pronounced neural activity spreading from the occipital to the parietal regions in the comparison between the target stimuli. Anomalous trichromats also exhibited similar temporal patterns toward the more salient target stimulus when each target stimulus was compared with the green standard stimulus, indicating comparable processing toward saliency across color vision types. Although a similarity was observed, neither behavioral nor neural responses in anomalous trichromats reflected saliency contrast differences. In addition, a comparative analysis of ERPs between color vision types did not reveal any distinct differences in either target stimulus. Given the large variations in color sensitivity in individuals with anomalous trichromacy, further investigation is required to understand the detailed neural processing in individuals with various color vision types.
The CUL4-DDB1-DCAF1 E3 ubiquitin ligase complex regulates PLK4 protein levels to prevent premature centriole duplication
Authors: Grossmann Josina , Kratz Anne-Sophie , Kordonsky Alina , Prag Gali , Hoffmann Ingrid 
Published: September 2023

AbstractCentrioles play important roles in the assembly of centrosomes and cilia. Centriole duplication occurs once per cell cycle and is dependent on polo-like kinase 4 (PLK4). To prevent centriole amplification, which is a hallmark of cancer, PLK4 protein levels need to be tightly regulated. Here, we show that the Cullin(CUL)4A/B-DDB1-DCAF1, CRL4DCAF1, E3 ligase targets PLK4 for degradation in human cells. DCAF1 binds and ubiquitylates PLK4 in G2 phase to prevent premature centriole duplication in mitosis. In contrast to the regulation of PLK4 by SCFβ-TrCP, the interaction between PLK4 and DCAF1 is independent of PLK4 kinase activity and mediated by polo-boxes 1 and 2 of PLK4, suggesting that DCAF1 promotes PLK4 ubiquitylation independently of β-TrCP. Thus, the SCFSlimb/β-TrCPpathway, targeting PLK4 for ubiquitylation based on its phosphorylation state and CRL4DCAF1, which ubiquitylates PLK4 by binding to the conserved PB1-PB2 domain, appear to be complementary ways to control PLK4 abundance to prevent centriole overduplication.
Three-dimensional markerless motion capture of multiple freely behaving monkeys for automated characterization of social behavior
Authors: Matsumoto Jumpei , Kaneko Takaaki , Kimura Kei , Negrete Salvador Blanco , Guo Jia , Suda-Hashimoto Naoko , Kaneko Akihisa , Morimoto Mayumi , Nishimaru Hiroshi , Setogawa Tsuyoshi , Go Yasuhiro , Shibata Tomohiro , Nishijo Hisao , Takada Masahiko , Inoue Ken-ichi 
Published: September 2023

AbstractGiven their high sociality and close evolutionary distance to humans, monkeys are an essential animal model for unraveling the biological mechanisms underlying human social behavior and elucidating the pathogenesis of diseases exhibiting abnormal social behavior. However, behavioral analysis of naturally behaving monkeys requires manual counting of various behaviors, which has been a bottleneck due to problems in throughput and objectivity. Here, we developed a three-dimensional markerless motion capture system that utilized multi-view data for robust tracking of individual monkeys and accurate reconstruction of the three-dimensional poses of multiple monkeys living in groups. Validation analysis in two monkey groups revealed that the system enabled the characterization of individual social dispositions and relationships through automated detection of various social events. Analyses of social looking facilitated the investigation of adaptive behaviors in a social group. These results suggest that this motion capture system will significantly enhance our ability to analyze primate social behavior.
Rat Wetness Response: Sensory Cues, Behavior & Fur-based Drying
Authors: Triumph Attah Augustine , Negrón-Moreno Paola N. , Amigo-Duran Macarena , Zhang Linghua , Kenngott Max , Brecht Michael , Clemens Ann M. 
Published: September 2023

AbstractIt never rains in standard lab-confinements; thus we have limited understanding of animal reactions to water and wetness. To address this issue, we sprayed water on different body parts of rats and measured drying and fur temperature by thermal imaging while manipulating behavior, sensory cues and fur. Spraying water on rats resulted in fur changes (hair clumping, apex formation), grooming, shaking, and scratching. Anesthesia abolished behavioral responses, interfered with fur changes, and slowed drying. Spraying water on different body parts resulted in differential behavioral drying responses. Spraying the head resulted in grooming and shaking responses; water evaporated twice as fast as water sprayed on the animal’s back or belly. We observed no effect of whisker removal on post-water-spraying behavior. In contrast, local anesthesia of dorsal facial skin reduced post-water-spraying behavioral responses. Shaving of head fur drastically enhanced post-water-spraying behaviors, but reduced water loss during drying; indicating that fur promotes evaporation, acting in tandem with behavior to mediate drying. Excised wet fur patches dried and cooled faster than shaved excised wet skin. Water was sucked into distal hair tips, where it evaporated. We propose the wet-fur-heat-pump-hypothesis; fur might extract heat required for drying by cooling ambient air.Abstract Figure
Double dissociation of spontaneous alpha-band activity and pupil-linked arousal on additive and multiplicative perceptual gain
Authors: Pilipenko April , Samaha Jason 
Published: September 2023

AbstractPerception is a probabilistic process dependent on external stimulus properties and one’s internal state. However, which internal states influence perception and via what mechanisms remain debated. We studied how spontaneous alpha-band activity (8-12 Hz) and pupil fluctuations impact visual detection and confidence across stimulus contrast levels (i.e., the contrast response function or CRF). We found that weak pre-stimulus alpha power induced an “additive” shift in the CRF, whereby stimuli were reported present more frequently at all contrast levels, including contrast of zero (i.e., false alarms). Conversely, pre-stimulus pupil size had a “multiplicative” effect on detection such that stimuli occurring during large pupil states (putatively corresponding to higher arousal) were perceived more frequently as contrast increased. Signal detection modeling reveals that alpha power changes detection criteria equally across the CRF but not detection sensitivity (d’) whereas pupil-linked arousal modulated sensitivity, particularly for higher contrasts. Interestingly, pupil size and alpha power were positively correlated, meaning that some of the effect of alpha on detection may be mediated by pupil fluctuations. However, pupil-independent alpha still induced an additive shift in the CRF corresponding to a criterion effect. Our data imply that weak alpha boosts detection and confidence by an additive factor, rather than by a multiplicative scaling of contrast responses, a profile which captures the effect of pupil-linked arousal. We suggest that alpha-power and arousal fluctuations have dissociable effects on behavior. Alpha reflects the baseline level of visual excitability, which can vary independent of arousal.Significance statementNearly a century ago, brain waves around 8-13 Hz (the “alpha-band”) were discovered and linked to visual processing and cortical arousal. However, the precise way that alpha activity shapes perception and relates to arousal is unsettled. We recorded pupillometry and EEG while subjects detected and reported confidence for visual stimuli with varying intensity. Stimuli occurring during states of strong alpha were seen less often, regardless of intensity level, suggesting alpha exerts subtractive inhibition on perception and confidence. Pupil size (a proxy for arousal) was found to correlate with alpha yet, surprisingly, has a different effect on perception. Small pupil lowered perceptual sensitivity more as stimulus intensity increased. Our findings reveal distinct effects of alpha activity and arousal on visual perception.
Competition and Synergy of Arp2/3 and Formins in Nucleating Actin Waves
Authors: Chua Xiang Le , Tong Chee San , Xŭ X.J. , Su Maohan , Xiao Shengping , Wu Xudong , Wu Min 
Published: September 2023

AbstractThe assembly and disassembly of actin filaments and their regulatory proteins are crucial for maintaining cell structure or changing physiological state. However, because of the tremendous global impact of actin on diverse cellular processes, dissecting the specific role of actin regulatory proteins remains challenging. In this study, we employ actin waves that propagate on the cortex of mast cell to investigate the interplay between formins and the Arp2/3 complex in the nucleating and turnover of cortical actin. Our findings reveal that the recruitment of FMNL1 and mDia3 precedes the Arp2/3 complex in cortical actin waves. Membrane and GTPase-interaction can drive oscillations of FMNL1 in an actin-dependent manner, but active Cdc42 waves or constitutively-active FMNL1 mutant can form without actin waves. In addition to the apparent coordinated assembly of formins and Arp2/3, we further reveal their antagonism, where inhibition of Arp2/3 complex by CK-666 led to a transient increase in the recruitment of formins and actin polymerization. Our analysis suggest that the antagonism could not be explained for the competition between FMNL1 and Arp2/3 for monomeric actin. Rather, it is regulated by a limited pool of their common upstream regulator, Cdc42, whose level is negatively regulated by Arp2/3. Collectively, our study highlights the multifaceted interactions, cooperative or competitive, between formins and Arp2/3 complex, in the intricate and dynamic control of actin cytoskeletal network.
Pharmacological modulation of developmental and synaptic phenotypes in human SHANK3 deficient stem cell-derived neuronal models
Authors: Thibaudeau Amandine , Schmitt Karen , François Louise , Chatrousse Laure , Hoffmann David , Cousin Loïc , Weiss Amélie , Vuidel Aurore , Jacob Christina B , Sommer Peter , Benchoua Alexandra , Wilbertz Johannes H 
Published: September 2023

AbstractOne sentence summaryThis study describes the use of SHANK3 deficient stem cell-derived neuronal models to screen and characterize small molecules that partially rescued developmental and synaptic defects related to Phelan-McDermid syndrome (PMDS).Phelan-McDermid syndrome (PMDS) arises from mutations in the terminal region of chromosome 22q13, impacting theSHANK3gene. The resulting deficiency of the postsynaptic density scaffolding protein SHANK3 is associated with autism spectrum disorder (ASD). We examined 12 different PMDS patient and CRISPR-engineered stem cell-derived neuronal models and controls and found that reduced expression of SHANK3 leads to neuronal hyperdifferentiation, increased synapse formation, and decreased neuronal activity. We performed automated imaging-based screening of 7,120 target-annotated small molecules and identified three compounds that rescued SHANK3-dependent neuronal hyperdifferentiation. One compound, Benproperine, rescued the decreased colocalization of Actin Related Protein 2/3 Complex Subunit 2 (ARPC2) with ß-actin and rescued increased synapse formation in SHANK3 deficient neurons when administered early during differentiation. Neuronal activity was only mildly affected, highlighting Benproperine’s effects as a neurodevelopmental modulator. This study demonstrates that small molecular compounds that reverse developmental phenotypes can be identified in human neuronal PMDS models.
Maternal IL-10 restricts fetal emergency myelopoiesis
Authors: Collins Amélie , Swann James W. , Proven Melissa A. , Patel Chandani M. , Mitchell Carl A. , Kasbekar Monica , Dellorusso Paul V. , Passegué Emmanuelle 
Published: September 2023

SUMMARYNeonates, in contrast to adults, are highly susceptible to inflammation and infection. Here we investigate how late fetal liver (FL) mouse hematopoietic stem and progenitor cells (HSPC) respond to inflammation, testing the hypothesis that deficits in engagement of emergency myelopoiesis (EM) pathways limit neutrophil output and contribute to perinatal neutropenia. We show that despite similar molecular wiring as adults, fetal HSPCs have limited production of myeloid cells at steady state and fail to activate a classical EM transcriptional program. Moreover, we find that fetal HSPCs are capable of responding to EM-inducing inflammatory stimuliin vitro, but are restricted by maternal anti-inflammatory factors, primarily interleukin-10 (IL-10), from activating EM pathwaysin utero. Accordingly, we demonstrate that loss of maternal IL-10 restores EM activation in fetal HSPCs but at the cost of premature parturition. These results reveal the evolutionary trade-off inherent in maternal anti-inflammatory responses that maintain pregnancy but render the fetus unresponsive to EM activation signals and susceptible to infection.HIGHLIGHTSThe structure of the HSPC compartment is conserved from late fetal to adult life.Fetal HSPCs have diminished steady-state myeloid cell production compared to adult.Fetal HSPCs are restricted from engaging in emergency myelopoiesis by maternal IL-10.Restriction of emergency myelopoiesis may explain neutropenia in septic neonates.eTOC BLURBFetal hematopoietic stem and progenitor cells are restricted from activating emergency myelopoiesis pathways by maternal IL-10, resulting in inadequate myeloid cell production in response to inflammatory challenges and contributing to neonatal neutropenia.
Shaping online and offline informal learning networks in off-site construction projects: a proximity perspective
Authors: Liu Kangning , Hwang Bon-Gang , Jia Jianyao , Man Qingpeng , Zhang Shoujian 
Published: September 2023

PurposeInformal learning networks are critical to response to calls for practitioners to reskill and upskill in off-site construction projects. With the transition to the coronavirus disease 2019 (COVID-19) pandemic, social media-enabled online knowledge communities play an increasingly important role in acquiring and disseminating off-site construction knowledge. Proximity has been identified as a key factor in facilitating interactive learning, yet which type of proximity is effective in promoting online and offline knowledge exchange remains unclear. This study takes a relational view to explore the proximity-related antecedents of online and offline learning networks in off-site construction projects, while also examining the subtle differences in the networks' structural patterns.Design/methodology/approachFive types of proximity (physical, organizational, social, cognitive and personal) between projects members are conceptualized in the theoretical model. Drawing on social foci theory and homophily theory, the research hypotheses are proposed. To test these hypotheses, empirical case studies were conducted on two off-site construction projects during the COVID-19 pandemic. Valid relational data provided by 99 and 145 project members were collected using semi-structured interviews and sociometric questionnaires. Subsequently, multivariate exponential random graph models were developed.FindingsThe results show a discrepancy arise in the structural patterns between online and offline learning networks. Offline learning is found to be more strongly influenced by proximity factors than online learning. Specifically, physical, organizational and social proximity are found to be significant predictors of offline knowledge exchange. Cognitive proximity has a negative relationship with offline knowledge exchange but is positively related to online knowledge exchange. Regarding personal proximity, the study found that the homophily effect of hierarchical status merely emerges in offline learning networks. Online knowledge communities amplify the receiver effect of tenure. Furthermore, there appears to be a complementary relationship between online and offline learning networks.Originality/valueProximity offers a novel relational perspective for understanding the formation of knowledge exchange connections. This study enriches the literature on informal learning within project teams by revealing how different types of proximity shape learning networks across different channels in off-site construction projects.
Machine learning approach for the prediction of mixed lubrication parameters for different surface topographies of non-conformal rough contacts
Authors: Prajapati Deepak Kumar , Katiyar Jitendra Kumar , Prakash Chander 
Published: September 2023

Purpose
This study aims to use a machine learning (ML) model for the prediction of traction coefficient and asperity load ratio for different surface topographies of non-conformal rough contacts.

Design/methodology/approach
The input data set for the ML model is generated using a mixed-lubrication model. Surface topography parameters (skewness, kurtosis and pattern ratio), rolling speed and hardness are used as input features in the multi-layer perceptron (MLP) model. The hyperparameter tuning and fivefold cross-validation are also performed to minimize the overfitting.

Findings
From the results, it is shown that the MLP model shows excellent accuracy (R2 > 90%) on the test data set for making the prediction of mixed lubrication parameters. It is also observed that engineered rough surfaces with high negative skewness, low kurtosis and isotropic surface patterns exhibit a significant low traction coefficient. It is also concluded that the MLP model gives better accuracy in comparison to the random forest regression model based on the training and testing data sets.

Originality/value
Mixed lubrication parameters are predicted by developing a regression-based MLP model. The machine learning model is trained using several topography parameters, which are vital in the mixed-EHL regime because of the lack of regression-fit expressions in previous works. The accuracy of MLP with random forest models is also compared.
The effect of social commerce attributes on customer engagement: an empirical investigation
Authors: Busalim Abdelsalam , Hollebeek Linda D. , Lynn Theo 
Published: September 2023

PurposeSocial commerce (s-commerce) offers community-based platforms that facilitate customer-to-customer interactions and the development of customers' social shopping-based experience. While prior research has addressed the role of customer engagement (CE) in boosting s-commerce-based sales and performance, insight into the effect of s-commerce attributes on CE remains tenuous. Addressing this gap, this study examines the role of specific s-commerce attributes (i.e. community, collaboration, interactivity and social dynamics) on CE, which is, in turn, proposed to impact customers' repurchase- and electronic word of mouth (eWOM) intention.Design/methodology/approachA web-based survey was deployed to target users of a popular s-commerce platform, Etsy.com. Partial least squares structural equation modeling (PLS-SEM) was, then, used to analyze the survey data collected from 390 users.FindingsThe results reveal that the four examined attributes positively affect CE. The findings also demonstrate CE's positive effect on customers' repurchase- and eWOM intention.Originality/valueThough CE has been identified as a key s-commerce performance indicator, little remains known about the role of specific s-commerce attributes in driving CE, as, therefore, explored in this research. Specifically, the authors examine the role of s-commerce-based community, collaboration, interactivity and social dynamics on CE. Their analyses also corroborate that CE, in turn, drives customers' post-purchase (i.e. repurchase/eWOM) intention. Managerially, our findings can be used to develop more engaging s-commerce platforms.
Enterprise social media usage and social cyberloafing: an empirical investigation using the JD-R model
Authors: Ma Liang , Zhang Xin , Yu Peng 
Published: September 2023

PurposeOne of the most important challenges confronting enterprise managers is that of controlling employees' social cyberloafing. The use of enterprise social media entails opportunities for cyberloafing. However, previous research on how enterprise social media use affects cyberloafing is rather limited. Using the job demands-resources (JD-R) model, this paper proposes a research model to investigate the relationship between enterprise social media usage and employees' social cyberloafing behavior.Design/methodology/approachStructural equation modeling was performed to test the research model and hypotheses. Surveys were conducted in an online platform in China, generating 510 employees' data for analysis.FindingsFirst, both public social media and private social media used for work-related and social-related purposes have a positive effect on employees' job engagement. Further, job engagement has a negative effect on employees' social cyberloafing. Second, the use of public social media for work-related and social-related purposes has no effect on employees' emotional exhaustion. However, work-related private social media usage has a negative effect on employees' emotional exhaustion, and social-related private social media usage has a positive effect on employees' emotional exhaustion. Further, employees' emotional exhaustion has a positive effect on employees' social cyberloafing. Third, there are significant differences in the effects of enterprise social media on employees' social cyberloafing between male and female employees.Originality/valueFirst, this paper contributes to the social cyberloafing literature by establishing a relationship between enterprise social media usage and social cyberloafing in relation to the dual influence mechanism. Second, it contributes to the JD-R model by clarifying how the use of enterprise social media with different motivations affects social cyberloafing through a mediation mechanism, namely, an enabling mechanism and a burden mechanism. Third, this paper also contributes to the social cyberloafing literature by revealing the boundary condition, namely gender, between enterprise social media use and employees' social cyberloafing.