bluuebunny/crossref_metadata_2025_split huggingface dataset:
Participatory action research to co-design a culturally appropriate COVID-19 risk communication and community engagement strategy in rural Pakistan
Authors: Moran Victoria Hall , Ceballos-Rasgado Marena , Fatima Sadia , Mahboob Usman , Ahmad Salman , McKeown Michael , Zaman Mukhtiar 
Published: April 2023

BackgroundCommunity engagement has shown to be fundamental component of the response to previous disease outbreaks. This study aimed co-design and implement a culturally appropriate COVID-19 risk communication and community engagement strategy with a resource-poor rural community in Northwest Pakistan.MethodsParticipatory Action Research (PAR) was conducted from January 2021 to March 2022. Five PAR meetings took place with community members (n = 30) to: (1) explore how the COVID-19 pandemic impacted on the community; (2) identify challenges to limit the spread of the virus; (3) identify and implement solutions to these challenges; and (4) highlight the enablers, challenges and knowledge of the cultural context needed to optimize safety during emergencies. Focus group discussions (N = 6) with community members not involved in the PAR meetings (N = 50) and children of the community (N = 26) were conducted following the PAR meetings. Thematic analysis of the PAR and focus group data was conducted.ResultsDelivery of messages on how to keep families safe, provision of personal protective equipment and improved water systems were part of the strategies taken by the community to create awareness and reduce the spread of COVID-19. Nine themes were identified: Attitudes to the pandemic: From skepticism to acceptance, Changing attitudes about vaccination: rumors and trust, COVID-19 and Faith, Social impact of the pandemic, Access to water, Resource mobilization: personal protective equipment, Spaces where collaborative effort can bring to solutions, Agents of change, and Empowerment of women.DiscussionThe participatory approach of this research allowed understanding of the challenges faced by the community to engage in behavior change strategies to reduce the spread of COVID-19 and enabled the community to find sustainable solutions. Engagement with the community empowered men and women to be agents of change and promoted necessary precautionary actions to reduce the risk of infection within their community.ConclusionParticipatory approach highlighted the importance of engaging with and integrating to local culture and values to overcome challenges such as gender imbalance and distrust. Findings of this study are relevant to others working in diverse cultural settings in similar crises events regardless of particular cultural variations.
Metformin mitigates SASP secretion and LPS-triggered hyper-inflammation in Doxorubicin-induced senescent endothelial cells
Authors: Abdelgawad Ibrahim Y. , Agostinucci Kevin , Sadaf Bushra , Grant Marianne K. O. , Zordoky Beshay N. 
Published: April 2023

Introduction: Doxorubicin (DOX), a chemotherapeutic drug, induces senescence and increases the secretion of senescence-associated secretory phenotype (SASP) in endothelial cells (ECs), which contributes to DOX-induced inflammaging. Metformin, an anti-diabetic drug, demonstrates senomorphic effects on different models of senescence. However, the effects of metformin on DOX-induced endothelial senescence have not been reported before. Senescent ECs exhibit a hyper-inflammatory response to lipopolysachharide (LPS). Therefore, in our current work, we identified the effects of metformin on DOX-induced endothelial senescence and LPS-induced hyper-inflammation in senescent ECs.Methods: ECs were treated with DOX ± metformin for 24 h followed by 72 h incubation without DOX to establish senescence. Effects of metformin on senescence markers expression, SA-β-gal activity, and SASP secretion were assessed. To delineate the molecular mechanisms, the effects of metformin on major signaling pathways were determined. The effect of LPS ± metformin was determined by stimulating both senescent and non-senescent ECs with LPS for an additional 24 h.Results: Metformin corrected DOX-induced upregulation of senescence markers and decreased the secretion of SASP factors and adhesion molecules. These effects were associated with a significant inhibition of the JNK and NF-κB pathway. A significant hyper-inflammatory response to LPS was observed in DOX-induced senescent ECs compared to non-senescent ECs. Metformin blunted LPS-induced upregulation of pro-inflammatory SASP factors.Conclusion: Our study demonstrates that metformin mitigates DOX-induced endothelial senescence phenotype and ameliorates the hyper-inflammatory response to LPS. These findings suggest that metformin may protect against DOX-induced vascular aging and endothelial dysfunction and ameliorate infection-induced hyper-inflammation in DOX-treated cancer survivors.
“It is something that gives us hope”: Lived experience among parents to children with cerebral palsy who are non-ambulant of the phenomenon physical activity, with or without the use of a novel dynamic standing device
Authors: Lauruschkus Katarina , Holmberg Robert , Tornberg Åsa B. 
Published: April 2023

IntroductionRegular physical activity confers health benefits for all. Parents commonly want their children to be physically active, and want to be physically active themselves, but children with cerebral palsy (CP) who are non-ambulant face challenges, and they need support to be physically active. Dynamic standing in the novel motorized assistive device Innowalk has positive effects in children who are non-ambulant—it gives them a chance to be physically active. The aim of this study was to explore the lived experience of physical activity of parents themselves and for their children with cerebral palsy who are non-ambulant.MethodsA descriptive inductive design with a hermeneutic phenomenological approach was used for the analysis of interviews with 11 parents of children with CP who are non-ambulant who participated in a study of exercise effects of dynamic standing.ResultsThe parents experienced physical activity for their children as being important but difficult, especially for their child, as described in Theme 1: “Being aware of health benefits while struggling with family time.” The children were perceived as being dependent on other people, the environment, and equipment for participating in physical activity, referring to Theme 2: “Being dependent.” The opportunity for their children to become physically active on a regular basis through an assistive device gave the parents hope for a better life, which formed Theme 3: “Getting hope in a challenging life situation.”ConclusionPhysical activity for children with CP who are non-ambulant is possible through an elaborate network of social relations and environmental conditions. Limiting the degree of dependence and containing the negative consequences of high a degree of dependence are vital in the support of physical activity. Relations, support, and assistive devices that strengthen empowerment and autonomy should be prioritized, and if this works, the experience of physical activity can be positive, giving families hope.
Soil quality and crop productivity under 34 years old long-term rainfed rice based cropping system in an Inceptisol of sub-tropical India
Authors: Biswas Sunanda , Singh Priya , Rahaman Riaj , Patil Kalyani V. , De Nirmal 
Published: April 2023

IntroductionSoil quality deterioration with the introduction of modern agriculture is a major threat to agricultural sustainability and food security and the problem is more aggravated specially under rainfed agriculture. Asessment of soil quality is a tortuous task as it can not be measured directly. The objective of the present investigation was to evaluate the effect of long-term fertilization and manuring on soil quality and identify the most sensitive indicators of assessing soil quality under rainfed rice based system.MethodsSoil samples were collected from selected six treatments viz. control, 100%NPK, 50%NPK, 50%FYM, 100%FYM and 50%NPK+FYM of 34 years old long-term fertilizer experiment with rainfed rice-lentil cropping system situated at BHU Varanasi, India.Results and discussionResult revealed that continuous organic manure application along with inorganic fertilizer increased soil organic carbon by 54.1% over control treatment.Principal component analysis (PCA) was done to screen out key indicators and mean weight diameter, available Fe, available N, potentially mineralizable N, available Zn, FDA hydrolase activity and Clay were selected as key indicators of soil quality. The highest soil quality index (SQI) of 0.95 was found in 50% NPK+FYM treatment. Regression analysis showed better agreement of equivalent rice yield and SQI (0.87). Therefore, the balanced fertilization with organic and inorganic fertilizers is important for sustainability of the rainfed rice-lentil cropping system and this practice may be recommended for rainfed rice based system of Indian Inceptisol.
The need and development for a value-added toolkit—A case study with Montana specialty fruit growers
Authors: Garg Sumedha , Jha Gaurav , Kim Sun-Hwa , Miller Zachariah , Kuo Wan-Yuan 
Published: April 2023

IntroductionCold-hardy small fruits and berries have the potential for specialty fruit growers in the Intermountain West, where the climate is not suited for conventional fruit crops. In the last seven years, approximately 50 varieties of cold-hardy, bush fruit types have been researched in western Montana.MethodHence with the increased small fruit and berry production, and interest of specialty fruit growers in value-added product development, this paper utilized participatory action research (PAR) to develop a value-added product development toolkit with specialty fruit growers and used an integrated logic model to discuss creating and implementing the toolkit. Firstly, we used an online survey to identify the needs and challenges of specialty fruit growers. Next, a value-added toolkit is drafted using the principles of design thinking and involving a student-grower partnership. Thirdly, the specialty fruit grower's interest in and feedback on the drafted toolkit is evaluated using focus group discussions and individuals interviews, and the results are used to revise the toolkit. Lastly, the short, medium and long-term outcomes for this toolkit are discussed using the logic model.ResultsFrom the survey, 61% of specialty fruit growers indicated an interest in value-added opportunities. Yet, focus group discussions and individual interviews found the biggest barriers to value-added product development are cost, resources, and environment. This indicated a co-created toolkit will be a beneficial solution. During focus group and individual interviews, the growers suggested including the toolkit as part of coursework in semester-long classes. This will address issues of continuity and funding.DiscussionOverall, this study deployed PAR methods to propose with Montana specialty fruit growers a solution to their increasing needs in value-added pursuits, implying short-term economic benefits but also long-term socio-ecological benefits. The participatory model of creating value-added resources presented by this paper can benefit other small-scale specialty crop growers in underserved regions.
Prognostic Values of Gene Copy Number Alterations in Prostate Cancer
Authors: Alfahed Abdulaziz , Ebili Henry Okuchukwu , Almoammar Nasser Eissa , Alasiri Glowi , AlKhamees Osama A. , Aldali Jehad A. , Al Othaim Ayoub , Hakami Zaki H. , Abdulwahed Abdulhadi M. , Waggiallah Hisham Ali 
Published: April 2023

Whilst risk prediction for individual prostate cancer (PCa) cases is of a high priority, the current risk stratification indices for PCa management have severe limitations. This study aimed to identify gene copy number alterations (CNAs) with prognostic values and to determine if any combination of gene CNAs could have risk stratification potentials. Clinical and genomic data of 500 PCa cases from the Cancer Genome Atlas stable were retrieved from the Genomic Data Commons and cBioPortal databases. The CNA statuses of a total of 52 genetic markers, including 21 novel markers and 31 previously identified potential prognostic markers, were tested for prognostic significance. The CNA statuses of a total of 51/52 genetic markers were significantly associated with advanced disease at an odds ratio threshold of ≥1.5 or ≤0.667. Moreover, a Kaplan–Meier test identified 27/52 marker CNAs which correlated with disease progression. A Cox Regression analysis showed that the amplification of MIR602 and deletions of MIR602, ZNF267, MROH1, PARP8, and HCN1 correlated with a progression-free survival independent of the disease stage and Gleason prognostic group grade. Furthermore, a binary logistic regression analysis identified twenty-two panels of markers with risk stratification potentials. The best model of 7/52 genetic CNAs, which included the SPOP alteration, SPP1 alteration, CCND1 amplification, PTEN deletion, CDKN1B deletion, PARP8 deletion, and NKX3.1 deletion, stratified the PCa cases into a localised and advanced disease with an accuracy of 70.0%, sensitivity of 85.4%, specificity of 44.9%, positive predictive value of 71.67%, and negative predictive value of 65.35%. This study validated prognostic gene level CNAs identified in previous studies, as well as identified new genetic markers with CNAs that could potentially impact risk stratification in PCa.
Energy Storage and Dissipation in Consecutive Tensile Load-Unload Cycles of Gum Metal
Authors: Golasiński Karol Marek , Staszczak Maria , Pieczyska Elżbieta Alicja 
Published: April 2023

Multifunctional β-titanium alloy Gum Metal, characterized by a relatively low elastic modulus, superelastic-like behavior and high strength, was subjected to cyclic tensile loadings. The characteristics of macroscopic scale energy storage and dissipation in the consecutive loading–unloading cycles were studied. Various kinds of energy components related to the alloy deformation process were determined experimentally and analyzed using thermodynamic relations. The values of the entire work needed to deform the alloy Wext, the work used for recoverable deformation Wrec consisting of the elastic deformation energy Wel , the superelastic-like energy Wpt , and the energy of thermoelastic effect Eth , were derived from the Gum Metal stress and temperature vs. strain curves. The irrecoverable mechanical energy Wir expended on plastic deformation, the dissipation energy Q, and finally the stored energy Es were estimated. The stored energy represents a change in the internal energy of the deformed material and is an essential measure of cold-worked state. The Es value turned out to be not large for the Gum Metal, which confirms the alloy low hardening property. The energy components determined for each of the 24 loading cycles enabled us to analyze various stages of the Gum Metal deformation process, including necking and damage.
Mechanism and Modulation of SidE Family Proteins in the Pathogenesis of Legionella pneumophila
Authors: Xie Yongchao , Zhang Yi , Wang Yong , Feng Yue 
Published: April 2023

Legionella pneumophila is the causative agent of Legionnaires’ disease, causing fever and lung infection, with a death rate up to 15% in severe cases. In the process of infection, Legionella pneumophila secretes over 330 effectors into host cell via the Dot/Icm type IV secretion system to modulate multiple host cellular physiological processes, thereby changing the environment of the host cell and promoting the growth and propagation of the bacterium. Among these effector proteins, SidE family proteins from Legionella pneumophila catalyze a non-canonical ubiquitination reaction, which combines mono-ADP-ribosylation and phosphodiesterase activities together to attach ubiquitin onto substrates. Meanwhile, the activity of SidE family proteins is also under multiple modulations by other effectors. Herein we summarize the key insights into recent studies in this area, emphasizing the tight link between the modular structure of SidE family proteins and the pathogen virulence as well as the fundamental mechanism and modulation network for further extensive research.
Response to Hypersalinity of Four Halophytes Growing in Hydroponic Floating Systems: Prospects in the Phytomanagement of High Saline Wastewaters and Extreme Environments
Authors: Barbafieri Meri , Bretzel Francesca , Scartazza Andrea , Di Baccio Daniela , Rosellini Irene , Grifoni Martina , Pini Roberto , Clementi Alice , Franchi Elisabetta 
Published: April 2023

Hypersaline environments occur naturally worldwide in arid and semiarid regions or in artificial areas where the discharge of highly saline wastewaters, such as produced water (PW) from oil and gas industrial setups, has concentrated salt (NaCl). Halophytes can tolerate high NaCl concentrations by adopting ion extrusion and inclusion mechanisms at cell, tissue, and organ levels; however, there is still much that is not clear in the response of these plants to salinity and completely unknown issues in hypersaline conditions. Mechanisms of tolerance to saline and hypersaline conditions of four different halophytes (Suaeda fruticosa (L.) Forssk, Halocnemum strobilaceum (Pall.) M. Bieb., Juncus maritimus Lam. and Phragmites australis (Cav.) Trin. ex Steudel) were assessed by analysing growth, chlorophyll fluorescence and photosynthetic pigment parameters, nutrients, and sodium (Na) uptake and distribution in different organs. Plants were exposed to high saline (257 mM or 15 g L−1 NaCl) and extremely high or hypersaline (514, 856, and 1712 mM or 30, 50, and 100 g L−1 NaCl) salt concentrations in a hydroponic floating culture system for 28 days. The two dicotyledonous S. fruticosa and H. strobilaceum resulted in greater tolerance to hypersaline concentrations than the two monocotyledonous species J. maritimus and P. australis. Plant biomass and major cation (K, Ca, and Mg) distributions among above- and below-ground organs evidenced the osmoprotectant roles of K in the leaves of S. fruticosa, and of Ca and Mg in the leaves and stem of H. strobilaceum. In J. maritimus and P. australis the rhizome modulated the reduced uptake and translocation of nutrients and Na to shoot with increasing salinity levels. S. fruticosa and H. strobilaceum absorbed and accumulated elevated Na amounts in the aerial parts at all the NaCl doses tested, with high bioaccumulation (from 0.5 to 8.3) and translocation (1.7–16.2) factors. In the two monocotyledons, Na increased in the root and rhizome with the increasing concentration of external NaCl, dramatically reducing the growth in J. maritimus at both 50 and 100 g L−1 NaCl and compromising the survival of P. australis at 30 g L−1 NaCl and over after two weeks of treatment.
Phenolic Profile of Castanea Bee Pollen from the Northwest of the Iberian Peninsula
Authors: Rodríguez-Flores María Shantal , Escuredo Olga , Seijo María Carmen , Rojo Sergio , Vilas-Boas Miguel , Falcão Soraia I. 
Published: April 2023

Bee pollen is a rich bee product, from the point of view of its nutritional and functional chemical characteristics. The chemical composition of bee pollen and its properties make this product an excellent food supplement for the human diet, due to its various functional bioactivities, such as having antioxidant, antibacterial, antifungal, and anti-inflammatory properties. These properties depend on the botanical origin of the bee pollen. Castanea sativa bee pollen is one of the most important types of pollen collected in the northwest of the Iberian Peninsula. Thus, the phenolic profile of Castanea bee pollen was featured in this study. For this, 11 samples of Castanea were selected through prior colorimetric separation using the CIELab* scale and verified with palynological analysis. Identification of the main phenol compounds was performed through LC/DAD/ESI-MSn analysis. The phenols compounds were quantified using calibration curves for caffeic acid, quercetin, and naringenin. The main results showed a profile formed of 19 compounds for all samples, although quantitative differences were found. Most of these compounds were phenolamides, with N1, N5, and N10-tricaffeoylspermidine being significantly (p < 0.05) the most abundant. Three isorhamnetin glycoside derivatives and one naringenin were also identified. The richness in phenolamides of Castanea bee pollen identified in this study suggests Castanea bee pollen as a functional food, owing to its healthy properties.
FORMATION OF CULTURAL AND EDUCATIONAL ENVIRONMENT OF ART INSTITUTIONS OF HIGHER EDUCATION
Authors: Shetelya N. 
Published: April 2023

In the article, based on the theoretical analysis of the leading groups of sources and work experience of the Communal Institution of Higher Education «Uzhgorod Institute of Culture and Arts» of the Zakarpattia Regional Council, the essence of the concept of «cultural and educational environment» of the artistic institution of higher education is investigated and substantiated, and the ways of forming such an environment are outlined.
Based on the works by O. Pilevych, O. Rebrova, H. Buchkivska, E. Mertens, and other scientists, it is found that the cultural-educational environment of art institutions of higher education is a complex integrated concept. It characterizes the spiritual space of the institution and the set of educational and cultural-educational pedagogical conditions and means those develop and shape the personality of the future specialist in the field of culture. The specifics (features of an art institution, the role of the environment in the process of education and upbringing of young people, a resource that enables their professional creative growth) and characteristics of such an environment are determined, and examples of the implementation of the tasks of its formation by means of the work of vocal and choral art collectives are given.
Conducting and researching the peculiarities of the formation of a cultural and educational environment in the conditions of an art institution of higher education made it possible to identify the main trends and characteristics of the development of such an environment. They are the following: the formation of the spiritual culture of youth, the assimilation of universal and national values, professional development, the formation of performing skills, the realization of individual creative abilities, and moral and aesthetic education in the conditions of the artistic collective and through the collective, readiness for active artistic and creative activity.