bluuebunny/crossref_metadata_2025_split huggingface dataset:
Complex epistatic interactions between ELF3, PRR9, and PRR7 regulates the circadian clock and plant physiology
Authors: Yuan Li , Avello Paula , Zhu Zihao , Lock Sarah C.L , McCarthy Kayla , Redmond Ethan J. , Davis Amanda M. , Song Yang , Ezer Daphne , Pitchford Jonathan W. , Quint Marcel , Xie Qiguang , Xu Xiaodong , Davis Seth J. , Ronald James 
Published: July 2023

AbstractCircadian clocks are endogenous timekeeping mechanisms that coordinate internal physiological responses with the external environment. EARLY FLOWERING3 (ELF3), PSEUDO RESPONSE REGULATOR (PRR9), and PRR7 are essential components of the plant circadian clock and facilitate entrainment of the clock to internal and external stimuli. Previous studies have highlighted a critical role for ELF3 in repressing the expression ofPRR9andPRR7. However, the functional significance of activity in regulating circadian clock dynamics and plant development is unknown. To explore this regulatory dynamic further, we firstly employed mathematical modelling to simulate the effect of theprr9/prr7mutation on theelf3circadian phenotype. These simulations suggested that simultaneous mutations inprr9/prr7could rescue theelf3circadian arrythmia. Following these simulations, we generated all Arabidopsiself3/prr9/prr7mutant combinations and investigated their circadian and developmental phenotypes. Although these assays could not replicate the results from the mathematical modelling, our results have revealed a complex epistatic relationship between ELF3 and PRR9/7 in regulating different aspects of plant development. ELF3 was essential for hypocotyl development under ambient and warm temperatures, while PRR9 was critical for root thermomorphogenesis. Finally, mutations inprr9andprr7rescued the photoperiod insensitive flowering phenotype of theelf3mutant. Together, our results highlight the importance of investigating the genetic relationship amongst plant circadian genes.
Microfluidic Quaking-Induced Conversion (Micro-QuIC) for Rapid On-Site Amplification and Detection of Misfolded Proteins
Authors: Lee Dong Jun , Christenson Peter R. , Rowden Gage , Lindquist Nathan C. , Larsen Peter A. , Oh Sang-Hyun 
Published: July 2023

AbstractProtein misfolding diseases, such as prion diseases, Alzheimer’s, and Parkinson’s, share a common molecular mechanism involving the misfolding and aggregation of specific proteins. There is an urgent need for point-of-care (POC) diagnostic technologies that can accurately detect these misfolded proteins, facilitating early diagnosis and intervention. Here, we introduce the Microfluidic Quaking Induced Conversion (Micro-QuIC), a novel acoustofluidic platform for the rapid and sensitive detection of protein misfolding diseases. We demonstrate the utility of our technology using chronic wasting disease (CWD) as a model system, as samples from wild white-tailed deer are readily accessible, and CWD shares similarities with human protein misfolding diseases. Acoustofluidic mixing enables homogeneous mixing of reagents in a high-Reynolds-number regime, significantly accelerating the turnaround time for CWD diagnosis. Our Micro-QuIC assay amplifies prions by an order of magnitude faster than the current gold standard, real-time quaking-induced conversion (RT-QuIC). Furthermore, we integrated Micro-QuIC with a gold nanoparticle-based, naked-eye detection method, which enables visual discrimination between CWD positive and negative samples without the need for a bulky fluorescence detection module. This integration creates a rapid, POC testing platform capable of detecting misfolded proteins associated with a variety of protein misfolding diseases.TOC graphic
SGIP1 binding to the α-helicalH9domain of cannabinoid receptor 1 promotes axonal surface expression
Authors: Fletcher-Jones Alexandra , Spackman Ellen , Craig Tim J. , Nakamura Yasuko , Wilkinson Kevin A. , Henley Jeremy M. 
Published: July 2023

AbstractEndocannabinoid signalling mediated by cannabinoid type 1 receptors (CB1Rs) is critical for homeostatic neuromodulation of both excitatory and inhibitory synapses. This requires highly polarised axonal surface expression of CB1R, but how this is achieved remains unclear. We previously reported that theH9domain in the intracellular C-terminus of CB1R contributes to polarised surface expression by an unknown mechanism. Here we show theH9domain binds to the endocytic adaptor protein SGIP1 to promote CB1R expression in the axonal membrane. Overexpression of SGIP1 increases CB1R axonal surface localisation but has no effect on CB1R lacking theH9domain (CB1RΔH9). Conversely, SGIP1 knockdown reduces axonal surface expression of CB1R but does not affect CB1RΔH9. Furthermore, SGIP1 knockdown diminishes CB1R-mediated inhibition of presynaptic Ca2+influx in response to neuronal activity. Together, these data advance mechanistic understanding of endocannabinoid signalling by demonstrating that SGIP1 interaction withH9underpins axonal CB1R surface expression to regulate presynaptic responsiveness.
Train for the job you want (or will be asked to take): envisioning the doctorate as preparation for equity-minded leadership in higher education
Authors: Pifer Meghan J. , Tevis Tenisha L. , Baker Vicki L. 
Published: July 2023

Purpose
The purpose of this study, nested within a broader study about higher education leadership, was to generate knowledge about the ways in which doctoral education prepared people for leadership roles in postsecondary institutions within the USA. At colleges and universities, there is an interest in ensuring diverse leadership teams and welcoming campus environments. Yet, the research demonstrates challenges for and underrepresentation among higher education leaders. One point of intervention is doctoral programs in higher education and related fields, given the professional socialization, identity formation and knowledge acquisition that occurs through the doctoral journey.

Design/methodology/approach
By conducting interviews with women who hold doctorates in the field of higher education and who took on new leadership roles at postsecondary institutions in the USA during the global health pandemic, the authors identified specific areas for which doctoral-level training and experiences may be helpful in supporting leader development.

Findings
The authors identified specific areas for which doctoral-level training and experiences may be helpful in supporting leader development. Based on those findings, the authors offer initial propositions about how doctoral programs might support the development of equity-minded leaders in higher education, which should be tested and refined through further research, theory development and application to practice.

Originality/value
This paper contributes by providing a focus on the ways in which doctoral programs can equitably train and develop equity-minded leaders for a range of career goals including but not limited to academic appointments in higher education.
Canada: Political Developments and Data in 2022
Authors: STEWART DAVID K. 
Published: July 2023

AbstractThere was no federal election in Canada in 2022, but the official opposition Conservatives ousted their leader and elected a new one. Additionally, the Liberals and New Democrats signed a Supply and Confidence Agreement that would protect the minority Liberal government from parliamentary defeat until 2025. Despite these significant developments, the major event was a convoy that descended on Ottawa to protest policies related to the COVID‐19 pandemic and was only removed after substantial police action and the declaration of the Emergencies Act.
Transcriptomes of human primary skin fibroblasts of healthy individuals reveal age‐associated mRNAs and long noncoding RNAs
Authors: Tsitsipatis Dimitrios , Martindale Jennifer L. , Mazan‐Mamczarz Krystyna , Herman Allison B. , Piao Yulan , Banskota Nirad , Yang Jen‐Hao , Cui Linna , Anerillas Carlos , Chang Ming‐Wen , Kaileh Mary , Munk Rachel , Yang Xiaoling , Ubaida‐Mohien Ceereena , Chia Chee W. , Karikkineth Ajoy C. , Zukley Linda , D'Agostino Jarod , Abdelmohsen Kotb , Basisty Nathan , De Supriyo , Ferrucci Luigi , Gorospe Myriam 
Published: July 2023

AbstractChanges in the transcriptomes of human tissues with advancing age are poorly cataloged. Here, we sought to identify the coding and long noncoding RNAs present in cultured primary skin fibroblasts collected from 82 healthy individuals across a wide age spectrum (22–89 years old) who participated in the GESTALT (Genetic and Epigenetic Signatures of Translational Aging Laboratory Testing) study of the National Institute on Aging, NIH. Using high‐throughput RNA sequencing and a linear regression model, we identified 1437 coding RNAs (mRNAs) and 1177 linear and circular long noncoding (lncRNAs) that were differentially abundant as a function of age. Gene set enrichment analysis (GSEA) revealed select transcription factors implicated in coordinating the transcription of subsets of differentially abundant mRNAs, while long noncoding RNA enrichment analysis (LncSEA) identified RNA‐binding proteins predicted to participate in the age‐associated lncRNA profiles. In summary, we report age‐associated changes in the global transcriptome, coding and noncoding, from healthy human skin fibroblasts and propose that these transcripts may serve as biomarkers and therapeutic targets in aging skin.
Haematological parameters of laying hens under different group size
Authors: Osadcha Yuliia 
Published: July 2023

AbstractThe relevance of this study lies in its potential implications for poultry farming practices and animal welfare. Understanding the impact of group size on the blood parameters of hens can provide valuable insights for optimising their health and well‐being in farming environments. The aim of the study was an analysis of haematological parameters of laying hens under the influence group size with the same stoking density in analogous cages by design. Hens were kept for 34 weeks in cages of multilevel batteries at different group sizes: I group—93 birds, II group—52 birds, III group—17 birds and IV group—9 birds. The study analysed blood samples from laying hens at 18 and 52 weeks of age. Results showed that reducing the group size from 93 to 52 birds led to a haematological status within the physiological norm, characterized by increased heterophils and decreased monocyte levels. Whereas, the decrease in group size to 17 birds was accompanied by a decrease in the hens' blood content of thrombocytes, an increase of heterophile, a decrease of monocytes and lymphocytes within the physiological norm, as well as an increase in the heterophiles to lymphocytes ratio.
Coptidis rhizoma and evodiae fructus against lipid droplet deposition in nonalcoholic fatty liver disease‐related liver cancer by AKT
Authors: Li Shenghao , Hao Liyuan , Deng Jiali , Zhang Junli , Hu Xiaoyu 
Published: July 2023

AbstractNonalcoholic fatty liver disease (NAFLD) is the most common liver disease in the world. NAFLD has become one of the major factors contributing to hepatocellular carcinoma (HCC) development. However, there are no clear targets and therapeutic drugs for NAFLD‐related liver cancer. This study explored the active compounds, target and mechanism of coptidis rhizoma and evodiae fructus in the treatment of NAFLD‐related liver cancer based on the network pharmacology and experimental verification. There were 455 intersection targets of NAFLD‐related liver cancer, and 65 drug‐disease common targets. AKT1 has the highest degree, indicating that it may be a key target of coptidis rhizoma and evodiae fructus in the treatment of NAFLD‐related liver cancer. The expression level of AKT1 was high in high‐risk group, and the overall survival rate was lower than that in low‐risk group. After oleic acid induction, p‐AKT expression and lipid droplet deposition were promoted in HepG2 cells. Quercetin and resveratrol reduced lipid droplet deposition in vivo. Moreover, quercetin inhibited p‐AKT expression, resveratrol both reduced the expression of p‐AKT and AKT. The overall findings suggested that quercetin inhibited AKT in the treatment of NAFLD‐related liver cancer.
Association of crown emergence angle and profile with dental plaque and inflammation at dental implants
Authors: Pelekos George , Chin Bonnie , Wu Xinyu , Fok Melissa R. , Shi Junyu , Tonetti Maurizio S. 
Published: July 2023

AbstractBackgroundThe shape of implant restorations is critical for function and aesthetics. It may also be important in peri‐implant tissue health preservation. This study aimed to associate the restorative contour of a single crown with marginal dental plaque accumulation, tissue inflammation and probing depths.MethodsSubjects with a single screw‐retained implant restoration were clinically examined. The presence of dental biofilm, tissue inflammation and probing pocket depths were the dependent variables. The emergence angle, profile and depth of the mucosal tunnel were measured on superimposed digital scans of the crown soft‐tissue complex, the removed crown mounted on an analogue and the soft tissue.ResultsOne hundred twenty two subjects (46.7% female, 68.9% never smokers, 77% with treated periodontitis and 52.5% participating in regular supportive peri‐implant care) were examined. The emergence angles at the mucosal margin were 15.3 ± 9.4°, 12.7 ± 8.5°, 31.3 ± 11.8° and 19.2 ± 9.8° for the mesial, distal, vestibular and oral aspects of the crowns. The largest emergence profile angles were observed on the vestibular aspect (74.6% of cases), reaching a maximum of 61.7°, and profiles were convex in 59% of cases. Generalized estimating equations indicated that the site‐specific platform‐level emergence angle and profile and depth of the mucosal tunnel were significantly associated with the presence of detectable plaque accumulation (p < .01) and bleeding on probing (p < .02).ConclusionsSubtle variations in the shape of the restorative crown are associated with biofilm accumulation and mucosal inflammation. These findings are important for 3D implant planning/positioning and preservation of peri‐implant tissue health.
Anne Barnard, her life and contribution to Curtis's Botanical Magazine
Authors: Rix Alison 
Published: July 2023

SummaryAnne Barnard (23 June 1833–19 January 1899), née Henslow, made around 125 illustrations for Curtis's Botanical Magazine, during the editorship of J.D. Hooker. Her life and botanical works are reviewed here, and examples of her original paintings are given.
Effect of thermal depolarization on the poling‐induced domain texture and piezoelectric properties in Mg‐doped NBT‐6BT
Authors: Cangini Laura , Wohninsland Andreas , Bremecker Daniel , Fulanović Lovro , Zhao Changhao , Rödel Jürgen 
Published: July 2023

AbstractRecently, poled Na0.50Bi0.50TiO3‐BaTiO3 (NBT‐BT)‐based polycrystalline materials have been characterized as possessing a high degree of poling‐induced domain texture in their remanent state. This finding is suggested to be the reason for their stable mechanical quality factor at high‐vibration velocity, making them promising candidates for high‐power applications. The materials in consideration are prone to self‐heating and thermal run‐away, particularly at slightly elevated temperatures. Therefore, this paper evaluates the temperature dependence of the poling‐induced domain texture of (Na0.47Bi0.47Ba0.06)TiO3 (NBT‐6BT) doped with 0.5 mol% of Mg as compared to undoped NBT‐6BT. Its influence on small‐signal, large‐signal, and high‐power properties was investigated. To obtain a fundamental understanding of crystal structure, in‐situ synchrotron measurements were conducted as function of temperature to establish a relationship between structure and piezoelectric properties of both Mg‐doped and undoped NBT‐6BT materials.
High‐performance KNN piezoceramics with reproducibility and chemical stability. Part II: Innovative melt‐reaction route
Authors: Vaschalde Lucile , Víllora Encarnación G. , Shimamura Kiyoshi 
Published: July 2023

AbstractIn the first part, a means to synthesize high‐density homogeneous (K,Na)NbO3 (KNN) ceramics with high piezoelectric performance and chemical stability at low spark‐plasma sintering temperature was found. However, these properties could not be obtained reproducibly, thus this second part is focused on solving this issue. Here, instead of doing a fine pulverization of raw materials prior to the reaction, the powders are ultimately mixed in the molten state and quenched to keep a single stoichiometry. A fast quenching is found to promote a better homogenization of the KNN phase upon annealing. Additionally, the coarse‐ground KNN powder is pre‐annealed before fine pulverization to preserve its high crystallinity. This innovative procedure prevents the formation of parasitic phases, leading with it to high‐performance undoped KNN ceramics (d33 ≈ 145 – 154 pC/N, kp ≈ 47–48% and kt ≈ 35 – 42%), while achieving both high reproducibility and chemical stability.
Feasibility of school‐based parent support groups for transition‐aged youth with intellectual disabilities
Authors: Lee Chung Eun , Choi Minsik 
Published: July 2023

AbstractBackgroundParents experience difficulties supporting children with intellectual disabilities when they exit the school system. One promising way to mitigate this problem is to set up school‐based parent support groups. This study aimed to examine the development and feasibility of school‐based parent support groups in South Korea designed to connect parents of transition‐aged youth with intellectual disabilities.MethodsThis qualitative study included 29 participants. Five focus groups and seven individual interviews were conducted with parents as co‐researchers. Data were analysed using thematic analysis.ResultsParticipants reported meaningful experiences after participating in the parent support groups. However, it is critical to consider the following components to promote their feasibility: clearly defined roles, small group meetings, school‐based initiatives, and collaboration.ConclusionsParent support groups could be a valuable way to promote parental empowerment. Implications for future practices are suggested.
Monitoring the consumption footprint of countries to support policy‐making: An assessment of data availability in Germany
Authors: Nuss Philip , Sanyé‐Mengual Esther , Sala Serenella 
Published: July 2023

AbstractThe European Green Deal and the German Resource Efficiency Programme both aim at decoupling resource consumption and associated environmental burdens from economic growth. Monitoring the progress of such policies requires robust estimates of environmental pressures and impacts, both from a domestic and a footprint perspective. Building on the life cycle assessment‐based consumption footprint (CoF) indicator, developed by the European Commission Joint Research Centre, we assess the environmental impacts of Germany's consumption in the areas of food, mobility, housing, household goods, and appliances during the period 2010–2018. A comparison between European and national consumption statistics revealed some differences in terms of data composition, granularity, consumption intensities, and calculated environmental impacts. Using national data sources results in slightly lower environmental impacts (e.g., due to differences in the assessment scope of national statistics) and requires some data preparation to match the CoF indicator. Emerging consumption trends can be highlighted using national data. Both data sources converge on main trends: Germany transgresses the safe operating space for several impact categories, with consumption of food, household goods, and mobility being the main drivers. Domestic impacts have decreased over time at the expense of outsourcing environmental pressures and impacts to other countries. The CoF indicator could complement resource monitoring frameworks and might be further aligned to the national context using country‐level consumption statistics and life cycle inventory data.
Configuring International Growth of Emerging Market Multinational Enterprises: A Compositional Springboarding View in the Context of India
Authors: Li Yi , Fan Di , Kumar Vikas , Ananthram Subramaniam 
Published: July 2023

AbstractThe rapid international growth of emerging market multinational enterprises (EMNEs) has become widely researched in the international management field. The compositional springboarding view (CSV) provides a theoretical understanding of amalgamation‐ambidexterity‐adaptability elements that impact EMNEs' internationalization. Here we utilize the CSV to conduct a configurational analysis of factors explaining Indian firms' rapid international growth. Using fuzzy‐set qualitative comparative analysis (fsQCA) of a survey‐archival data set matched with a sample of listed manufacturing firms from India, we identify five configurational solutions for Indian multinational enterprises (MNEs) to achieve international growth. We further develop a taxonomy of Indian MNEs' CSV configurations supporting rapid international growth that we name as amalgamator, adapter, augmentor, explorer, and springboarder. Through the configurational approach, we extend the EMNE literature in the international management field by exploring the efficacy and complementarity of compositional springboarding factors that enable Indian MNEs' rapid international growth. We also contribute to the organization theory and strategy literatures by addressing the tension alleviation between reconfiguring existing resources and creating new ones. Our theoretical extension opens new opportunities for CSV to guide strategic analyses across various resource reuse and creation scenarios.
The happy personality revisited: Re‐examining associations between Big Five personality traits and subjective well‐being using meta‐analytic structural equation modeling
Authors: Busseri Michael A. , Erb Emily M. 
Published: July 2023

AbstractObjective/BackgroundUsing meta‐analytic structural equation modeling (MASEM), we examined the link between Big Five personality traits and subjective well‐being (SWB), operationalized as three separate components and as a latent factor indicated by life satisfaction (LS), positive affect (PA), and negative affect (NA). PA and NA were assessed based on frequency of a broad range of affective experiences, rather than intensity of high arousal affective experiences, thus excluding studies using the Positive and Negative Affect Schedule.Method35 samples were included, encompassing 22,135 participants from 14 countries, in which all eight variables were assessed.ResultsCorrelations among personality traits were moderate, on average, and the latent SWB factor had strong loadings from all three components. Personality traits together explained substantial variance in LS, PA, and NA, and in the latent SWB factor, with unique predictive effects on the latent factor from each personality trait except openness. Associations between personality traits and SWB components were fully accounted for by a latent SWB factor, with one exception: A specific association was found between neuroticism and unique variance in NA.ConclusionsThe present findings provide new insights concerning the notion of a ‘happy personality’ in showing that Big Five personality traits have unique associations with an underlying sense of SWB.
The EU and Justice in Palestine: An Interview with Grace O'Sullivan
Authors: Gaess Roger 
Published: July 2023

AbstractGrace O'Sullivan of the Irish Green Party, affiliated with the European Green Party, has been a member of the European Parliament from Ireland for the South constituency since July 2019. In the European Parliament, she is a member of delegations on Palestine and Mercosur. O'Sullivan previously served as a senator in Ireland and was a sponsor of the Control of Economic Activity (Occupied Territories) Bill 2018, which sought to prohibit the import of goods produced in illegal settlements. O'Sullivan, an environmental activist, spent 20 years with Greenpeace and worked on the crew of the Rainbow Warrior in 1985, when it was bombed in New Zealand by French intelligence. She was interviewed by Roger Gaess via email in April and May 2023.