bluuebunny/crossref_metadata_2025_split huggingface dataset:
Production of Plant Garbage Juice and Its Effects on Plant Growth, Antibacterial Activity and Soil Reconditioning
Authors: Meem Jerin Shikder , Akter Mst Rony , Mahmud Shahin , Ali Md Rayhan , Hasan Mohammad Kamrul , Mohiuddin A K M 
Published: January 2025

Plant Garbage Juice (PGJ) is a highly concentrated bio-molecular solution. It contains water and bio-molecules such as acetic acid, sugar, proteins, alcohol, as well several enzymes like protease, amylase, lipase, and papain. It is promoted as a multifunctional solution for agricultural and domestic needs. In this investigation, two types of PGJ solutions were prepared to observe their various effects. The first one was prepared with mixed vegetable peels (PGJ-1) and the second with citrus peels (PGJ-2). Both solutions were acidic in nature, pH and EC (Electrical Conductivity) were detected as 4.3, 5.3 in PGJ-1, and 3.3, 2.58 in PGJ-2, respectively. PGJ-1 solution was applied to Aloe chinensis, a dwarf cultivar, and the growth rate was found to be higher than the control. In treatment 2 (T-2) of PGJ-1, the 2:1000 solution with water showed the best results for plant growth activities. The leaves of Aloe chinensis was found to turned brown green to dark green and the leaf thickness increased from 3.1 mm to 12.6 mm. The trash enzymes of PGJ solutions (I and II) exhibited antibacterial action against gram-positive and gram-negative bacteria, especially against pathogenic drug-resistant E. coli. PGJ-1 solution showed the highest zone of inhibition of 16 mm comparable to commercial antibiotic disk. PGJ solutions that have a catalytic enzyme showed the soil reconditioning process, such as a reduction of soil salinity. The mean of 20 soil samples showed that PGJ decreased the pH from 7.78 to 7.43. Similarly, the mean EC value of 20 soil samples also decreased from 0.45 ms/cm to 0.15 ms/cm. The pH and EC values of an alkaline sample also decreased significantly from 8.05 to 7.31 and 1.85 to 0.12, respectively. The results determined indicate that PGJ is a cost-effective plant growth-promoting factor and an eco-friendly substitute for minimizing global threats to agriculture.
Plant Tissue Cult. & Biotech. 34(2): 237-245, 2024 (December)
Pemberian Makanan Tambahan (PMT) Dan Pendidikan Ibu Berhubungan Dengan Status Gizi Balita
Authors: Deo Florianus Alva , Metrikayanto Wahyu Dini , Susmini Susmini 
Published: January 2025

Balita kelompok rentan gizi, penderita gizi buruk dapat mengalami pertumbuhan jangka pendek dan mengalami keterbatasan pertumbuhan dan perkembangan jaringan tubuh, pikiran dan otak, sehingga mempengaruhi tingkat kesadaran otak.Tujuan penelitian mengetahui hubungan pemberian makanan tambahan (PMT) dengan pendidikan ibu dan status gizi balita di Desa Pandansari Kecamatan Ngantang Provinsi Malang. Desain penelitian yang digunakan adalah cross-sectional. Populasi penelitian ini adalah 267 anak prasekolah, sampel penelitian 73 responden dengan menggunakan simple random sampling. Instrumenmenggunakan angket dan lembar observasi. Uji analisis chi-square. Hasil penelitian menunjukkan bahwa sebagian besar balita tidak mendapatkan makanan tambahan (52,1%), hampir separuh ibu yang memiliki balita berpendidikan SMA (42,5%), dan hampir separuh balita mengalami gizi buruk (49,3%). Hasil uji chi-square p=0,000 (X1) dan p-value=0,001 (X2), dapat disimpulkan bahwa ada hubungan antara pemberian makanan tambahan (PMT) dan pendidikan ibu dengan status gizi balita di Desa Pandansari Kecamatan Ngantang Kabupaten Malang. Direkomendasikan penelitian selanjutnya mengkaji faktor-faktor lain yang mungkin mempengaruhi pelaksanaan program PMT
Analisis Laporan Keuangan Untuk Mengukur Kinerja Keuangan PT. Teknologi Karya Digital Nusa Tbk
Authors: Kurniawan April 
Published: January 2025

This research analyzes the financial performance of PT. Teknologi Karya Digital Nusa Tbk during the period of 2019-2022 using financial ratios. The purpose of this study is to assess the company's financial performance through liquidity, solvency, activity, and profitability ratios. The method used in this research is descriptive quantitative. The data used in this study are secondary data obtained from the company's financial statements. The analysis shows a significant improvement in liquidity ratios, with the Current Ratio increasing from 110% in 2019 to 254% in 2022. Solvency ratios show fluctuation but reflect an overall decrease in the proportion of debt. The activity ratios indicate enhanced operational efficiency, while profitability ratios demonstrate consistent growth in net profit margin, return on equity, and return on investment. These findings suggest that PT. Teknologi Karya Digital Nusa Tbk has strengthened its financial position, improving efficiency and profitability amidst industry challenges.

Penelitian ini menganalisis kinerja keuangan PT. Teknologi Karya Digital Nusa Tbk selama periode 2019-2022 menggunakan rasio keuangan. Tujuan penelitian ini adalah untuk menilai kinerja keuangan perusahaan melalui rasio likuiditas, solvabilitas, aktivitas, dan profitabilitas. Metode yang digunakan dalam penelitian ini adalah deskriptif kuantitatif. Data yang digunakan dalam penelitian ini adalah data sekunder yang diperoleh dari laporan keuangan perusahaan. Analisis menunjukkan peningkatan signifikan dalam rasio likuiditas, dengan Current Ratio meningkat dari 110% pada 2019 menjadi 254% pada 2022. Rasio solvabilitas menunjukkan fluktuasi tetapi mencerminkan penurunan proporsi utang secara keseluruhan. Rasio aktivitas mencerminkan peningkatan efisiensi operasional, sementara rasio profitabilitas menunjukkan pertumbuhan yang konsisten dalam Nett Profit Margin, Return On Equity, dan Return On Investment. Temuan ini menunjukkan bahwa PT. Teknologi Karya Digital Nusa Tbk telah memperkuat posisinya secara finansial, meningkatkan efisiensi dan profitabilitas di tengah tantangan industri.
Managing complexity in cross-cohort classrooms: strategies for effective implementation of real-life projects
Authors: Das Syaamantak , Iyer Sridhar 
Published: January 2025

IntroductionReal-life project-based courses aim to enhance student learning by integrating authentic projects into the curriculum. However, the complexity of these projects, due to their VUCA (Volatile, Uncertain, Complex, and Ambiguous) nature, can pose significant challenges, especially for students new to the concepts and skills being taught. This study addresses the overarching question: “How can a systematic model be developed and implemented to effectively manage teaching and learning through developing a real-life project in a hybrid cross-cohort environment?”.MethodsThe study was conducted within an Instructional Systems Design (ISD) course in a hybrid cross-cohort class structure. A diverse class of 27 students with varying backgrounds participated in the course. Data were collected through surveys, interviews, and instructor observations. The implementation process involved multiple student cohorts, primary stakeholders of the project, subject matter experts, and instructors.ResultsThe study resulted in the development of the proposed 3 Square model to manage hybrid cross-cohort learning environments. Students engaged in developing a real-life project—a public awareness website for Persons with Disabilities (PwD) in a university environment. They navigated the complexities of coordinating within a cross-cohort classroom and developed critical skills such as autonomous decision-making and effective communication. Outcomes included efficient time management, achievement of project objectives, and tangible learning outcomes such as mastering ISD topics, using new tools, and working collaboratively in a group.DiscussionThe findings highlight the importance of leveraging diverse student knowledge and experience to manage challenges in cross-cohort classrooms for complex projects. Practical implications are discussed for instructional design and teaching strategies in diverse educational settings, emphasizing the need for systematic approaches to facilitate real-life problem-based learning tasks.ConclusionThis study provides a retrospective evolution of a systematic model for hybrid cross-cohort learning environments. It identifies teaching-learning challenges, determines appropriate instructional strategies, and evaluates the effectiveness of solutions in facilitating real-life project-based learning. The insights gained contribute to managing diverse educational settings and enhancing instructional design practices.
Association between sperm DNA fragmentation index and recurrent pregnancy loss: results from 1485 participants undergoing fertility evaluation
Authors: Yao Guanying , Dou Xianchao , Chen Xiaozhu , Qi Haolin , Chen Jianling , Wu Peiwei , Li Jialu , Liang Shuang , Han Zhongjiang , Bai Shun , Hu Xu 
Published: January 2025

ObjectiveSeveral male factors have been reported to play a role in recurrent pregnancy loss (RPL). The aim of this study is to explore the relationship between semen parameters, sperm DNA fragmentation index (DFI) and RPL.MethodA total of 1485 participants were recruited from a university hospital between April 2020 and August 2022. Six hundred and thirtyfour men from couples with RPL were assigned to the case group, while 851 men from couple without RPL who underwent fertile evaluation were assigned to the control group. Semen parameters including sperm DNA fragmentation, were assessed. ResultsNo statistically significant differences in semen parameters, sperm kinematics and DFI were observed between the case group and the control group. A higher proportion of men in the case group had a DFI &gt; 30% compared to those in the control group; however, this difference was not statistically significant. Restricted cubic spline analysis revealed no significant non-linear relationships between continuous DFI and risk of RPL.ConclusionOur study indicates that there is no significant relationship between DFI and RPL risk. Further prospective studies are needed to explore the impact of DFI on fertility outcomes in couples experiencing RPL.
Co-methylation networks associated with cognition and structural brain development during adolescence
Authors: Jensen Dawn , Chen Jiayu , Turner Jessica A. , Stephen Julia M. , Wang Yu-Ping , Wilson Tony W. , Calhoun Vince D. , Liu Jingyu 
Published: January 2025

IntroductionTypical adolescent neurodevelopment is marked by decreases in grey matter (GM) volume, increases in myelination, measured by fractional anisotropy (FA), and improvement in cognitive performance.MethodsTo understand how epigenetic changes, methylation (DNAm) in particular, may be involved during this phase of development, we studied cognitive assessments, DNAm from saliva, and neuroimaging data from a longitudinal cohort of normally developing adolescents, aged nine to fourteen. We extracted networks of methylation with patterns of correlated change using a weighted gene correlation network analysis (WCGNA). Modules from these analyses, consisting of co-methylation networks, were then used in multivariate analyses with GM, FA, and cognitive measures to assess the nature of their relationships with cognitive improvement and brain development in adolescence.ResultsThis longitudinal exploration of co-methylated networks revealed an increase in correlated epigenetic changes as subjects progressed into adolescence. Co-methylation networks enriched for pathways involved in neuronal systems, potassium channels, neurexins and neuroligins were both conserved across time as well as associated with maturation patterns in GM, FA, and cognition.DiscussionOur research shows that correlated changes in the DNAm of genes in neuronal processes involved in adolescent brain development that were both conserved across time and related to typical cognitive and brain maturation, revealing possible epigenetic mechanisms driving this stage of development.
Titanium nanotubes modulate immunophenotyping and cytokine secretion of T cells via IL-17A: a bioinformatic analysis and experimental validation
Authors: Yin Jingju , Liao Yunyang , Liu Shaofeng , Che Bangwei , Zhu Hanghang , Yang Bingbing , Shi Bin 
Published: January 2025

ObjectWe aim to explore the immunomodulatory properties of T cells on different titanium nanotubes and the key immunological factors involved in this process.MethodsTranscriptome data from GEO database of healthy people and healthy implants were used to analyze cell infiltration and factor distribution of adaptive immune using bioinformatics tools. T cells from activated rat were cultured on titanium nanotubes that were prepared by anodization with different diameters (P-0, NT15-30 nm, NT40-100 nm, NT70-200 nm). The proliferation and expressions of the main transcription factors and cytokines of T-cells were detected. Magnetic bead sorting of CD3+ T cells and transcriptome sequencing were performed to explore the signaling pathways and key immune factors that may influence the related immune responses.ResultsBioinformatics analysis showed that healthy peri-implant tissues were enriched by the most of T-cell subtypes. T-cell-mediated adaptive immunological responses involved IL-17A. On the third day, the NT15 and NT40 groups showed significantly higher pro-proliferative effects than the NT70 group (P&lt;0.05). Notably, the NT40 group exhibited the lowest T-bet expression (P&lt;0.05) along with the highest levels of Rorγt, Gata3, and Foxp3(P&lt;0.05), followed by the NT15 group. Additionally, the NT40 group demonstrated reduced RANKL, TNF-α, and IL-6 (P&lt;0.05) and increased OPG and IL-10 (P&lt;0.05). Meanwhile, the NT15 group had lower IFN-γ expression(P&gt;0.05) but higher IL-4, and TGF-β1 expressions(P&lt;0.05). Differential expressed genes (DGEs) of T-cell related to the morphologies of titanium nanotubes were mostly enriched in the IL-17 signaling pathway mediated by IL-17A/F. Gene and protein expressions indicated that the NT40 group had the highest secretion in IL-17A of T cells.ConclusionTitanium nanotube morphologies in medium (100 nm) and small (30 nm) sizes significantly influence T cell differentiation and immune factor secretion, with T-cell-derived IL-17A likely playing a key regulatory role.
Tumor-associated lymphatic vessel density is a postoperative prognostic biomarker of hepatobiliary cancers: a systematic review and meta-analysis
Authors: Li Jin , Liang Yu-Bo , Wang Qing-Bo , Li Yu-Kai , Chen Xing-Ming , Luo Wan-Ling , Lakang Yawhan , Yang Zi-Sheng , Wang Yan , Li Zhi-Wei , Ke Yang 
Published: January 2025

PurposeThis study aimed to investigate whether tumor-associated lymphatic vessel density (LVD) could predict the survival of patients with hepato-biliary-pancreatic (HBP) cancers after radical resection.MethodsA systematic search was conducted using PubMed, Embase, and Cochrane Library from the inception to July 31, 2024 for literature that reported the role of LVD in overall survival (OS) and recurrence-free survival (RFS) of patients with HBP cancers after radical resection.ResultsTen studies with 761 patients were included for the meta-analysis. The results indicated that a higher level of LVD was associated with worse OS (hazard ratio, HR = 2.87, 95% CI 1.63 to 5.04) and worse RFS (HR = 3.18, 95% CI 1.41 to 7.17) in HBP cancers. Subgroup analysis based on pathological types revealed that a higher level of LVD was significantly related to worse OS in hepatocellular carcinoma (HCC) (HR = 2.35, 95% CI 1.16 to 4.78), cholangiocarcinoma (HR = 4.65, 95% CI 1.70 to 12.70), and gallbladder cancer patients (HR = 4.64, 95% CI 1.37 to 15.71). The levels of LVD were not significantly associated with OS in pancreatic adenocarcinoma patients after radical resection (HR = 1.08, 95% CI 0.61 to 1.89). Similarly, a higher level of LVD was significantly associated with worse RFS in HCC (HR = 1.92, 95% CI 1.01 to 3.65) and cholangiocarcinoma patients (HR = 4.54, 95% CI 2.10 to 9.83).ConclusionsA higher level of LVD was a biomarker for the prediction of worse OS and RFS in patients with hepatobiliary cancers after radical resection.Systematic review registrationhttps://www.crd.york.ac.uk/prospero/, identifier CRD42024571167.
Integrating single cell analysis and machine learning methods reveals stem cell-related gene S100A10 as an important target for prediction of liver cancer diagnosis and immunotherapy
Authors: Huang Shenjun , Tu Tingting 
Published: January 2025

BackgroundHepatocellular carcinoma (LIHC) poses a significant health challenge worldwide, primarily due to late-stage diagnosis and the limited effectiveness of current therapies. Cancer stem cells are known to play a role in tumor development, metastasis, and resistance to treatment. A thorough understanding of genes associated with stem cells is crucial for improving the diagnostic precision of LIHC and for the advancement of effective immunotherapy approaches.MethodThis research combines single-cell RNA sequencing with machine learning techniques to identify vital stem cell-associated genes that could act as prognostic biomarkers and therapeutic targets for LIHC. We analyzed various datasets, applying negative matrix factorization alongside machine learning algorithms to reveal gene expression patterns and construct diagnostic models. The XGBoost algorithm was specifically utilized to identify key regulatory genes related to stem cells in LIHC, and the expression levels and prognostic significance of these genes were validated experimentally.ResultsOur single-cell analysis identified 16 differential prognostic genes associated with liver cancer stem cells. Cluster analysis and diagnostic models constructed using various machine learning techniques confirmed the significance of these 16 genes in the diagnosis and immunotherapy of LIHC. Notably, the XGBoost algorithm identified S100A10 as the stem cell-related gene most relevant to the prognosis of LIHC patients. Experimental validation further supports S100A10 as a potential prognostic marker for this cancer type. Additionally, S100A10 shows a positive correlation with the stem cell marker POU5F1.ConclusionThe results of this study highlight S100A10 as an essential predictor for liver cancer diagnosis and treatment response, particularly regarding immunotherapy. This research offers valuable insights into the molecular mechanisms underlying LIHC and suggests S100A10 as a promising target for enhancing treatment outcomes in liver cancer patients.
Correlation between hepatic steatosis severity diagnosed by ultrasound and metabolic indexes in elderly patients with MAFLD
Authors: Liang Zhitang , Huang Renhao , Zhang Lingyun 
Published: January 2025

ObjectiveTo explore the connection between metabolic parameters and the severity of hepatic steatosis determined through ultrasound in elderly individuals with metabolic dysfunction-associated fatty liver disease (MAFLD).Methods4,663 senior individuals who were 65 years of age or older were included in this research. They were examined physically at the Ninghai Street Community Health Service Center in Yantai City between June 7, 2021, and October 15, 2021. There were two categories of individuals identified: the MAFLD group (n = 2,985) and the non-MAFLD group (n = 1,678). Based on liver ultrasonography results, individuals in the MAFLD group were further separated into three groups: mild (n = 2,104), moderate (n = 766), and severe (n = 115). To identify indicators of risk for the severity of hepatic steatosis, metabolic data was contrasted between the groups employing logistic regression.ResultsIn comparison to the non-MAFLD group, the MAFLD group showed significantly elevated levels of body mass index (BMI), blood pressure, gender, age, lipid profile, alanine transaminase (ALT), and fasting blood glucose (FBG; p &lt; 0.05). Among individuals with MAFLD, there was a positive correlation between BMI, FBG, ALT, and aspartate transaminase (AST) levels and the severity of hepatic steatosis (p &lt; 0.05). Logistic regression analysis indicated that BMI, female gender, FBG, ALT, triglycerides (TG), and serum uric acid (SUA) constituted risk factors for increased severity of hepatic steatosis in MAFLD.ConclusionThe severity of hepatic steatosis in elderly MAFLD patients is significantly correlated with female gender, BMI, ALT, FBG, TG, and SUA.
Ligating the varicose vein trunk for bleeding stomal varices in portal hypertension: a case report
Authors: Sun Jialiang , Tan Zhanhai , Zhang Jifa 
Published: January 2025

IntroductionIn colostomy-related complications, variceal hemorrhage particularly induced by cirrhosis and portal hypertension is seldom encountered. The onset of peristome variceal hemorrhage necessitates swift and effective intervention to prevent potentially life-threatening outcomes such as hemorrhagic shock and recurrent stoma bleeding.Case presentationThis report details a case of repeated varicose vein hemorrhage around the stoma in a patient with liver cirrhosis. Abdominal enhanced CT images revealed that the stomal varices originated from a branch of the inferior mesenteric vein, with vein balls encompassing the stoma. The patient was acquired with successful hemostasis through high ligation of the various vein primary trunk, and stripping of the vein balls around stoma, along with intracutaneously suturing of the sub-abdominal wall varicose veins. When the stoma bag was changed it was observed that the skin surrounding the stoma was flat, the mucosa was red, and the varicose venous mass had vanished. After 2 months of follow-up, the stomal function was doing well without any rebleeding episodes.ConclusionIn this instance, decompensated cirrhosis led to stomal varices and recurrent bleeding, which was initially managed with local compression and suture therapy but resulted in rebleeding. Our team’s approach through blocking the primary trunk of variceal vein via a minimal incision under local anesthesia may offer a new treatment strategy for patients with poor liver function who cannot withstand the trauma associated with general anesthesia and major surgery.
Long-term trends in the burden of pulmonary arterial hypertension in China and worldwide: new insights based on GBD 2021
Authors: Xu Zhehao , Ding Jia , Liang Ruiyun , Xie Shuangfeng 
Published: January 2025

BackgroundPulmonary arterial hypertension (PAH) poses a significant health challenge globally, with China experiencing a notable increase in its burden. Understanding the trends and factors contributing to PAH is crucial for developing effective public health strategies.MethodsThis study utilized data from the Global Burden of Disease (GBD) 2021 database to estimate the burden of PAH in China and worldwide from 1990 to 2021. A Bayesian age-period-cohort (BAPC) model was employed to analyze differences in PAH burden across age, gender, and time periods, and to project global epidemiological trends until 2036.ResultsFrom 1990 to 2021, the incidence and prevalence of PAH in China increased by 80.59% and 86.74%, respectively. The age-standardized incidence rate (ASIR) and age-standardized prevalence rate (ASPR) showed an annual percentage change (AAPC) of -0.07% and 0.25%, respectively. Conversely, the age-standardized disability-adjusted life year (DALY) rate and age-standardized mortality rate (ASMR) have been declining since 1990, with AAPC of -1.90% and -1.26%, respectively. Females and the 50–70 years age group experienced a higher PAH burden compared to males. Projections indicate that ASPR, ASMR, and age-standardized death rate (ASDR) will stabilize with minimal variation over the next decade.DiscussionThe findings highlight the age-related burden of PAH in China, particularly affecting older populations and women. The projected stabilization of PAH metrics over the next decade underscores the need for continued monitoring and targeted interventions. This study’s comprehensive analysis of PAH burden over three decades provides valuable insights for policymakers and healthcare providers, necessitating concerted efforts to address this critical health issue.