bluuebunny/crossref_metadata_2025_split huggingface dataset:
The effect of additional orange Pangkep (Citrus maxima) peel oil on characteristics and microbial inhibition of corn flour-based edible film
Authors: Idayani  , Hasizah A , Syarifuddin A 
Published: June 2023

Abstract
The edible film is a thin layer of hydrophilic materials made of proteins, carbohydrates, and fats that replaces plastic packaging. This study was carried out to determine the effect of adding the Pangkep orange peel (Citrus maxima) oil on edible films based on corn flour. Treatments applied in this research were concentration of corn flour (2%, 4%, and 6% (w/v)) and concentration of Pangkep orange peel (Citrus maxima) oil (1%, 1.5%, and 2% (v/v)). Measurement parameters were tensile strength, elongation, thickness, moisture content, and inhibition of Escherichia coli and Staphylococcus aureus. The results showed that the composition of Pangkep orange oil (Citrus maxima) and corn flour had a significant effect on the elongation, thickness, moisture, and inhibition of Escherichia coli and Staphylococcus aureus. The best formula treatment was obtained with a combination treatment containing 4% corn flour and 1% Pangkep orange peel oil (Citrus maxima).
Effect of supplementing cellulase, carnitine and lemuru fish oil in feed on chemical composition of Muscovy duck meat
Authors: Sudibya  , Salsabila  , Rusdiyana E , Rinanto Y , Utomo S B , Siswanto B 
Published: June 2023

Abstract
Muscovy duck in Indonesia has good potential to be developed as a meat producer, but currently its production and performance are still relatively low. additional feed is needed to increase it, such as cellulase enzymes, L-carnitine, and lemuru fish oil. where this combination, enzymes can increase nutrient digestibility, fish oil which is rich in unsaturated fatty acids and L-carnitine as a metabolic facilitator needed to oxidize long chain fatty acids. This research was conducted to investigate the effect of supplementing cellulase, carnitine and fish oil in feed on performance, EPA and DHA composition of Muscovy duck meat. In total, 120 Muscovy ducks in grower period were allocated to 4 dietary treatments with 5 replicates of 6 ducks. The treatments consisted of T0 = basal ration, T1 = T0 supplemented with 0.1% cellulase, T2 = T1 supplemented with L-carnitine 40 ppm, and T3 = T2 supplemented with 4% lemuru fish oil. The dietary treatments were fed for 90 days. The results of study showed that the effects of supplementing fish oil and L-carnitine in rations containing cellulase enzyme have a significant value (P<0.01) on the performance (feed consumption, daily body weight gain, feed conversion and IOFC) of male Muscovy duck grower phase and have a significant value (P<0.01) on EPA and DHA level in Muscovy duck meat. The supplementation can decrease feed consumption, meanwhile it increase daily body weight gain, improve feed conversion ratio, increase IOFC value, EPA and DHA level.
Oysterman and Refugee
Authors: Ho Denise Y. 
Published: June 2023

Abstract
This article examines the Hong Kong–China maritime border since 1949, when the establishment of the People’s Republic of China and the onset of the Cold War turned the 1898 imperial boundary into an ideological line, one that nonetheless remained porous to ties of trade and kinship. It focuses on the oyster-producing communities in the tidelands of the Pearl River estuary, showing how oystermen—some of them also refugees—faced security threats exacerbated by the Cultural Revolution while they also leveraged the borderland’s opportunities. The oyster industries are a case study in two forms of agricultural production: traditional land and labor relations on the Hong Kong coast, and collective agriculture in China’s socialist period, followed by decollectivization in the reform era. By the end of the Mao years in 1976, China’s oyster industry well exceeded that of Hong Kong’s, but both systems were vulnerable to industrial pollution. In the reform era and after, China’s oystermen built upon persistent networks to navigate their position between Hong Kong, Shenzhen, and the Pearl River Delta. The prosperity of the China oysterman, rather than the Hong Kong refugee, illustrates the inversion of the border’s valence from a colonial past to a postsocialist future.
“Improper and Almost Rebellious Conduct”
Authors: Mishler Max 
Published: June 2023

Abstract
The 1833 Slavery Abolition Act provided for the gradual emancipation of eight hundred thousand human beings. It also confirmed the sovereignty of King-in-Parliament over all people residing in British dominions and resolved a long-standing dispute over whether enslaved people were private property or royal subjects entitled to legal safeguards. This debate first emerged in the late eighteenth century but acquired additional urgency following the 1807 abolition of the slave trade, when attempts to mitigate slavery through the enactment of ameliorative statutes and procedural reforms encouraged enslaved people to petition magistrates for redress in cases of abuse. Slaves vigorously defended their newly granted rights to bodily protection, sustenance, and family preservation through the instigation of legal complaints against overseers, managers, and slave owners. By the 1820s, enslaved litigants across Britain’s empire were publicly and collectively petitioning colonial magistrates to intercede on their behalf. The judicialization of quotidian battles over the terms of enslavement refashioned colonial social relations, affirmed enslaved people’s status as British subjects, and generated volumes of case files that circulated back to the metropole, where the Colonial Office cited them in critical assessments of slave law and where abolitionists used them to press for immediate emancipation. Enslaved people’s legal activism was operationally antislavery; it eroded the power of colonial enslavers and prodded Parliament to pass the 1833 Abolition Act.
Practical Reason and Private Law: Some Sketches
Authors: Finnis John 
Published: June 2023

Abstract
Our common law rejected Roman law’s treatment of contractual and tortious obligations as property, but radicalized property rights by making their object abstract entities such as estates and trusts. By dealing in such entities’ existence or non-existence, and in the validity/fallacy of arguments about them, Property minimizes practical reasoning (about ends, means, the rightful, permitted, wrongful …), for a practical reason—to advance valuable ends such as stability and security and their fruits: prosperity (in principle for all), and independence in face of public power and oligarchical (or indeed democratic) groupthink. Part II illustrates this in a simple Trusts case.
Tort, however, is private law-rules/principles for enforcing originally moral obligations to compensate for violating essentially moral but formally conventional and derivatively/technically legal obligations (of respect and care). Part III illustrates Tort’s private-law/moral character in dialectic with Goldberg and Zipursky. Part IV sketches Tort’s relevance to compensating for aspects and consequences of regulators’ failures to exercise morally/conventionally due care in executing public-law duties/powers to regulate makers, distributors or mandators of inherently dangerous medical means.
Comparing the impact of mixed-culture microbial communities and fecal transplant on the intestinal microbiota and metabolome of weaned piglets
Authors: Rahman Rajibur , Fouhse Janelle M , Prisnee Tausha L , Ju Tingting , Diether Natalie E , Willing Benjamin P 
Published: June 2023

Abstract
Fecal microbiota transplantation (FMT) is an emerging technique for modulating the pig microbiota, however, donor variability is one of the major reasons for inconsistent outcomes across studies. Cultured microbial communities may address some limitations of FMT; however, no study has tested cultured microbial communities as inocula in pigs. This pilot study compared the effects of microbiota transplants derived from sow feces to cultured mixed microbial community (MMC) following weaning. Control, FMT4X, and MMC4X were applied four times, while treatment FMT1X was administered once (n = 12/group). On postnatal day 48, microbial composition was modestly altered in pigs receiving FMT in comparison with Control (Adonis, P = .003), mainly attributed to reduced inter-animal variations in pigs receiving FMT4X (Betadispersion, P = .018). Pigs receiving FMT or MMC had consistently enriched ASVs assigned to genera Dialister and Alloprevotella. Microbial transplantation increased propionate production in the cecum. MMC4X piglets showed a trend of higher acetate and isoleucine compared to Control. A consistent enrichment of metabolites from amino acid metabolism in pigs that received microbial transplantation coincided with enhanced aminoacyl-tRNA biosynthesis pathway. No differences were observed among treatment groups for body weight or cytokine/chemokine profiles. Overall, FMT and MMC exerted similar effects on gut microbiota composition and metabolite production.
Telomere Length, Health, and Mortality in a Cohort of Older Black South African Adults
Authors: Gao Sarah , Rohr Julia K , de Vivo Immaculata , Ramsay Michele , Krieger Nancy , Kabudula Chodziwadziwa W , Farrell Meagan T , Bassil Darina T , Harriman Nigel W , Corona-Perez Diana , Pesic Katarina , Berkman Lisa F 
Published: June 2023

Abstract
Telomere length (TL) may be a biomarker of aging processes as well as age-related diseases. However, most studies of TL and aging are conducted in high-income countries. Less is known in low- and middle-income countries (LMICs) such as South Africa, where life expectancy remains lower despite population aging. We conducted a descriptive analysis of TL in a cohort of older adults in rural South Africa. TL was assayed from venous blood draws using quantitative polymerase chain reaction (T/S ratio). We examined the correlation between TL and biomarkers, demographic characteristics, mental/cognitive health measures, and physical performance measures in a subsample of the Wave 1 2014–2015 “Health and Aging in Africa: A Longitudinal Study of an INDEPTH Community in South Africa” (HAALSI) cohort (n = 510). We used logistic regression to measure the association between TL and mortality through Wave 3 (2021–2022). In bivariate analyses, TL was significantly correlated with age (r = −0.29, p &lt; .0001), self-reported female sex (r = 0.13, p = .002), mortality (r = −0.1297, p = .003), diastolic blood pressure (r = 0.09, p = .037), pulse pressure (r = −0.09, p = .045), and being a grandparent (r = −0.17, p = .0001). TL was significantly associated with age (β = −0.003; 95% confidence interval [CI] = −0.005, −0.003). TL was significantly associated in unadjusted multivariate analyses with mortality, but the relationship between TL and mortality was attenuated after adjusting for age (odds ratio [OR] = 0.19; 95% CI = 0.03, 1.27) and other covariates (OR = 0.17; 95% CI = 0.02, 1.19). Our study is the first analysis of TL in an older adult South African population. Our results corroborate existing relationships between TL and age, sex, cardiometabolic disease, and mortality found in higher-income countries.
The seismogenic structure of March 2021 Tyrnavos (central Greece) doublet (Mw 6.3 andMw 6.0), constrained by aftershock locations and geodetic data
Authors: Papadimitriou Eleftheria , Karakostas Vasileios , Papazachos Constantinos , Foumelis Michael , Kiratzi Anastasia , Pikridas Christos , Bonatis Pavlos , Kostoglou Anastasios , Kourouklas Christos , Scordilis Emmanuel , Bitharis Stylianos , Paradisopoulou Parthena , Panou Areti , Galanis Odysseas , Karagianni Eleni , Vamvakaris Domenikos , Karagianni Ioanna , Kkallas Charalambos , Chatzis Nikolaos , Chatzipetros Alexandros , Fotiou Aristidis , Ventouzi Chrisanthi , Grendas Ioannis , Kementzetzidou Despoina , Karakaisis George , Hatzidimitriou Panagiotis 
Published: June 2023

SUMMARYThe Northern Thessaly Basin in central Greece ranks amongst the most well pronounced extensional (graben) basins in the backarc Aegean Sea region, with well-mapped faults having an ∼E–W orientation, compatible with the ongoing predominant ∼N–S extension. The southern margin of the basin is bounded by major faults associated with strong (M6 to M7) earthquakes, whereas along its northern margin, strong events are more scarce, in the documented catalogues. Along this northern margin, a weak, albeit persisting foreshock activity, culminated within 3 d, to an Mw 6.3 earthquake on 3 March 2021 associated with a 15-km-long NE dipping fault segment. It was followed the next day, by the second Mw 6.0 main shock associated with a 13-km-long NE dipping fault segment and 9 d later by an Mw 5.5 earthquake associated with an 8-km-long SW dipping fault segment, with its aligned epicentres, showcasing the cascade type activation of adjacent fault segments. The sequence, evolved to be very productive, with aftershocks extending ∼50 km along a ∼NW–SE trending narrow seismic zone. All events indicate pure normal faulting, with an NNE–SSW oriented extensional axis, oblique to our previous consensus of the prevalence of ∼N–S extension. This observation documents that inherited fault fabric can be reactivated within the modern tectonic stress field. We use high-quality seismological data, alongside Interferometric Synthetic Aperture Radar (InSAR) methodology and Global Navigation Satellite System (GNSS) data, to study the temporal and spatial evolution of the sequence, and to provide inferred kinematic models that describe the complexity of the seismic process, in terms of heterogeneous slip distribution, activated fault planes, fault geometry and displacement field. Cross-sections show that the activity defines the crustal seismogenic layer at depths between 5 and 10 km, associated with low-angle fault segments dipping to the NE. Other faults, both antithetic and secondary ones, appear active and accommodated aftershocks clusters. Using our preferred finite fault source model, we calculated the changes of Coulomb failure stress on the neighbouring faults.
Designing an infographic webtool for public health
Authors: Cullen Riley , Heitkemper Elizabeth , Backonja Uba , Bekemeier Betty , Kong Ha-Kyung 
Published: June 2023

Abstract

Objective
To create and evaluate a public health informatics tool, Florence, for communicating information to the public.

Materials and Methods
This user-centered design study included 3 phases: (1) an interview and survey study with public health practitioners to assess needs for creating infographics; (2) the application of assessment findings and public health-motivated design guidelines to the design and development of a public health-specific infographic design tool; and (3) a feasibility and usability study to evaluate the feasibility and usability of the tool.

Results
In phase 1, participants noted the importance of tailoring infographics to an audience and wanted flexible tools along with design guidance to help make fewer design decisions. In phase 2, we developed a prototype tool with: (1) layout and functionality familiar to PH users, (2) quick and intuitive ways to add and modify data in visualizations, and (3) health-focused visual elements. In phase 3, participants found Florence to be usable, providing an intuitive and straightforward experience, and that the focus on public health was useful.

Discussion
Based on needs assessments and existing literature, we created Florence along with public health practitioners to address their domain specific needs, ultimately leading to a tool that participants in our study deemed useful. Future research can build on our work to develop user-centered tools to meet their needs.

Conclusion
Infographics are important for public health communication. Creating user-centered solutions to address the unique needs of public health can support communication efforts.
A Different Perspective on the Characterization of a New Degradation Product of Flibanserin With HPLC–DAD–ESI-IT-TOF-MSn and Its Pharmaceutical Formulation Analysis With Inter-Laboratory Comparison
Authors: Geven Aysun , Özcan Saniye , Levent Serkan , Can Nafiz Öncü 
Published: June 2023

Abstract

Background
Flibanserin (FLB) was first synthesized as an antidepressant drug; however, due to its enhancing effects on sexual activity, it was approved for treatment of hypoactive sexual desire disorder in women in 2015.

Objective
The aim of this study was to develop a new and fully validated HPLC method for analysis of FLB in pharmaceutical formulations besides its degradation products, and identification of possible formation mechanisms by using HPLC-DAD-ESI-IT-TOF-MSn.

Method
The HPLC separation was achieved in a Supelco Ascentis® Express series phenyl hexyl column (100 × 4.6 mm, ID 2.7 µm). The mobile phase was acetonitrile–ammonium acetate solution (50:50, v/v, 10 mM, pH 5.4) mixture, which was pumped at the rate of 0.5 mL/min. Chromatography, detection, and structural identification was performed by using a LCMS-IT-TOF instrument (Shimadzu, Japan).

Results
1–(2-(4–(3-hydroxy-5-(trifluoromethyl)phenyl)piperazine-1-yl)ethyl)-1,3-dihydro-2H-benzo[d]imidazol-2-one is proposed as a novel degradation product, with a mass of 407.1695 and a formula of C20H21F3N4O2 with a margin of error about 0.001 ppm. The developed method is applicable with 98% accuracy within the 2.5–50.0 µg/mL range. The LOD and LOQ were about 500 ng/mL and 1.50 µg/mL, respectively. The transferability and variation between laboratories were tested by inter-laboratory comparison and evaluated with one-way analysis of variance.

Conclusions
A novel FLB degradation product, which was produced under oxidative forced degradation conditions was observed and identified for the first time; in addition, the formation kinetics of the degradation product besides decomposition of FLB was studied. Furthermore, an inter-laboratory comparison was carried out, and application of the proposed method on a pseudo Addyi® (Sprout Pharmaceuticals, Inc.) sample was tested using both instrument configurations.

Highlights
A novel stability-indicating assay method was developed and fully validated according to the International Council on Harmonization (Q2) R1 for the analysis of FLB in the pharmaceutical preparations. A new degradation product was identified in the oxidative forced degradation condition and characterized using HPLC–DAD–ESI-IT-TOF-MS3. Moreover, the possible mechanism and the formation kinetic of the degradation product were revealed. In addition, the developed method was transferred to another LC-PDA instrument for inter-laboratory comparison. Finally, the current method was applied to a pseudo formulation of Addy in both instruments, and ANOVA was applied for evaluation.
Can dietary magnesium sources and buffer change the ruminal microbiota composition and fermentation of lactating dairy cows?
Authors: Lobo Richard R , Arce-Cordero Jose A , Agustinho Bruna C , Ravelo Ana D , Vinyard James R , Johnson Mikayla L , Monteiro Hugo F , Sarmikasoglou Efstathios , Roesch Luiz Fernando W , Jeong Kwang Cheol C , Faciola Antonio P 
Published: June 2023

Abstract
Magnesium oxide (MgO) is one of the most used Mg supplements in livestock. However, to avoid relying upon only one Mg source, it is important to have alternative Mg sources. Therefore, the objective of this study was to evaluate the effects of the interaction of two Mg sources with buffer use on the ruminal microbiota composition, ruminal fermentation, and nutrient digestibility in lactating dairy cows. Twenty lactating Holstein cows were blocked by parity and days in milk into five blocks with four cows each, in a 2 × 2 factorial design. Within blocks, cows were assigned to one of four treatments: 1) MgO; 2) MgO + Na sesquicarbonate (MgO+); 3) calcium–magnesium hydroxide (CaMgOH); 4) CaMgOH + Na sesquicarbonate (CaMgOH+). For 60 d, cows were individually fed a corn silage-based diet, and treatments were top-dressed. Ruminal fluid was collected via an orogastric tube, for analyses of the microbiota composition, volatile fatty acids (VFA), lactate, and ammonia nitrogen (NH3–N). The microbiota composition was analyzed using V4/16S rRNA gene sequencing, and taxonomy was assigned using the Silva database. Statistical analysis was carried out following the procedures of block design analysis, where block and cow were considered random variables. Effects of Mg source, buffer, and the interaction between Mg Source × Buffer were analyzed through orthogonal contrasts. There was no interaction effect of the two factors evaluated. There was a greater concentration of NH3–N, lactate, and butyrate in the ruminal fluid of cows fed with CaMg(OH)2, regardless of the buffer use. The increase in these fermentation intermediates/ end-products can be explained by an increase in abundance of micro-organisms of the genus Prevotella, Lactobacillus, and Butyrivibrio, which are micro-organisms mainly responsible for proteolysis, lactate-production, and butyrate-production in the rumen, respectively. Also, dietary buffer use did not affect the ruminal fermentation metabolites and pH; however, an improvement of the apparent total tract digestibility of dry matter (DM), organic matter (OM), neutral fiber detergent (NDF), and acid fiber detergent (ADF) were found for animals fed with dietary buffer. In summary, there was no interaction effect of buffer use and Mg source, whereas buffer improved total tract apparent digestibility of DM and OM through an increase in NDF and ADF digestibility and CaMg(OH)2 increased ruminal concentration of butyrate and abundance of butyrate-producing bacteria.
Regulatory functional role of NLRP3 inflammasome during Mycoplasma hyopneumoniae infection in swine
Authors: Zhang Yan , Liu Bo , Said Abdelrahman , Xie Jinwen , Tian Fengrong , Cao Zongxi , Chao Zhe , Li Feng , Li Xin , Li Shuguang , Liu Hailong , Wang Wenxiu 
Published: June 2023

Abstract
Mycoplasma hyopneumoniae causes enzootic pneumonia, a highly contagious respiratory disease in swine that causes significant economic losses worldwide. It is unknown whether the nucleotide oligomerization domain-like receptor (NLR) family pyrin domain containing 3 (NLRP3) inflammasome regulates the immune response in swine during M. hyopneumoniae infection. The current study utilized an in vivo swine model of M. hyopneumoniae infection to investigate the regulatory functional role of the NLRP3 inflammasome during M. hyopneumoniae infection. Notable histopathological alterations were observed in M. hyopneumoniae-infected swine tissues, which were associated with an inflammatory response and disease progression. Swine M. hyopneumoniae infection was associated with an increase in the expression of the NLRP3 inflammasome, which stimulated pro-inflammatory cytokines such as tumor necrosis factor-alpha, interleukin 18, and interleukin 1 beta (IL-1β). The impact of the NLRP3 inhibitor, MCC950 on NLRP3 and pro-inflammatory cytokines in M. hyopneumoniae-infected swine was examined to investigate the relationship between the NLRP3 inflammasome and M. hyopneumoniae infection. Taken together, our findings provide strong evidence that the NLRP3 inflammasome plays a critical regulatory functional role in M. hyopneumoniae infection in swine.