bluuebunny/crossref_metadata_2025_split huggingface dataset:
Display Selection of a Hybrid Foldamer–Peptide Macrocycle
Authors: Dengler Sebastian , Howard Ryan T. , Morozov Vasily , Tsiamantas Christos , Huang Wei‐En , Liu Zhiwei , Dobrzanski Christopher , Pophristic Vojislava , Brameyer Sophie , Douat Céline , Suga Hiroaki , Huc Ivan 
Published: September 2023

AbstractExpanding the chemical diversity of peptide macrocycle libraries for display selection is desirable to improve their potential to bind biomolecular targets. We now have implemented a considerable expansion through a large aromatic helical foldamer inclusion. A foldamer was first identified that undergoes flexizyme‐mediated tRNA acylation and that is capable of initiating ribosomal translation with yields sufficiently high to perform an mRNA display selection of macrocyclic foldamer–peptide hybrids. A hybrid macrocyclic nanomolar binder to the C‐lobe of the E6AP HECT domain was selected that showed a highly converged peptide sequence. A crystal structure and molecular dynamics simulations revealed that both the peptide and foldamer are helical in an intriguing reciprocal stapling fashion. The strong residue convergence could be rationalized based on their involvement in specific interactions with the target protein. The foldamer stabilizes the peptide helix through stapling and through contacts with key residues. These results altogether represent a significant extension of the chemical space amenable to display selection and highlight possible benefits of inserting an aromatic foldamer into a peptide macrocycle for the purpose of protein recognition.
Application of polysaccharide based modified ground calcium carbonate in wet‐end of papermaking
Authors: Chatterjee Trinadha , Negi Satya Dev , Janbade Anuradha , Tyagi Sanjay , Gupta Manoj Kumar , Bhardwaj Shubhang , Kaur Prabhjot 
Published: September 2023

AbstractTo enhance the ash in paper without reducing its strength property, ground calcium carbonate (GCC) filler was modified using polysaccharide prior to its addition at wet‐end of papermaking. Till now, modification of GCC filler using chitosan acetate (CH.A) is not explored. The novelty lies in showing the effect of CH.A as GCC filler modifier and its application in papermaking. The results showed that ash of paper get increased by around 18%, 53% and 63% after adding GCC modified using cationic starch (C.S) @ 20 kg/t of GCC, CH.A @ 5 kg/t of GCC and CH.A @ 10 kg/t of GCC respectively, in comparison to that attained by paper using unmodified GCC at the same dose at wet‐end of papermaking. The breaking length, burst factor and tear factor of paper having 11% ash (made using unmodified GCC) was found to be 2485 m, 21.73 and 47.63, respectively. The paper having 18% ash (made using GCC modified by CH.A 10 kg/t of GCC) showed breaking length, burst factor and tear factor as 2364 m, 22.24 and 46.72, respectively. This approach of using CH.A in papermaking will help the papermaker to improve profit and quality of paper.
Site‐specific crosslinking and assembly of tetrameric β‐glucuronidase improve glycyrrhizin hydrolysis
Authors: Wang Qibin , Wang Yingying , Jian Xing , Wang Ning , Li Chun , Liu Hu 
Published: September 2023

AbstractIn this study, eight nonconserved residues with exposed surfaces and flexible conformations of the homotetrameric PGUS (β‐glucuronidase from Aspergillus oryzae Li‐3) were identified. Single‐point mutation into cysteine enabled the thiol‐maleimide reaction and site‐specific protein assembly using a two‐arm polyethylene glycol (PEG)‐maleimide crosslinker (Mal2). The Mal2(1k) (with 1 kDa PEG spacer)‐crosslinked PGUS assemblies showed low crosslinking efficiency and unimproved thermostability except for G194C‐Mal2(1k). To improve the crosslinking efficiency, a lengthened crosslinker Mal2(2k) (with 2 kDa PEG spacer) was used to produce PGUS assembly and a highly improved thermostability was achieved with a half‐life of 47.2–169.2 min at 70°C, which is 1.04–3.74 times that of wild type PGUS. It is found that the thermostability of PGUS assembly was closely associated with the formation of inter‐tetramer assembly and intratetramer crosslinking, rather than the PEGylation of the enzyme. Therefore, the four‐arm PEG‐maleimide crosslinker Mal4(2k) (with 2 kDa PEG spacer) was employed to simultaneously increase the inter‐tetramer assembly and intratetramer crosslinking, and the resulting PGUS assemblies showed further improved thermostabilities compared with Mal2(2k)‐crosslinked assemblies. Finally, the application of PGUS assemblies with significantly improved thermostability to the bioconversion of GL proved that the PGUS assembly is a strong catalyst for glycyrrhizin (GL) hydrolysis in industrial applications.
Enhancing green brand equity through environmental reputation: The important of green brand image, green brand trust, and green brand loyalty
Authors: Tran Nguyen Khanh Hai 
Published: September 2023

AbstractWhile previous research have established the advantages of environmental reputation (ER) in the realm of brand development, this article examines the role of ER on the value of green brands in the green food production industry, based on customer perceptions in a newly emerging market, using insights from signal theory and the Hierarchy of Effects Model (HEM). The study data was obtained from consumers who regularly make purchasing decisions for green‐labeled food in Vietnam. The study results suggest that the ER is a critical factor to develop green brand equity (GBE). This is achieved by developing the green brand image (GBI), establishing the green brand trust (GBT), and fostering the green brand loyalty (GBL). The results provide a significant contribution to promoting the development of green brand value in customers' awareness by encouraging activities and programs that enhance ER to strengthen GBI, GBT, and GBL towards green food brands. In addition to the findings, the research has limitations and suggests future research directions.
Current insights into the oncogenic roles of lncRNA LINC00355
Authors: Shen Jinze , Su Xinming , Pan Ming , Wang Zehua , Ke Yufei , Wang Qurui , Dong Jingyin , Duan Shiwei 
Published: September 2023

AbstractLong noncoding RNAs (lncRNAs) are a class of nonprotein‐coding transcripts that are longer than 200 nucleotides. LINC00355 is a lncRNA located on chromosome 13q21.31 and is consistently upregulated in various cancers. It regulates the expression of downstream genes at both transcriptional and posttranscriptional levels, including eight microRNAs (miR‐15a‐5p, miR‐34b‐5p, miR‐424‐5p, miR‐1225, miR‐217‐5p, miR‐6777‐3p, miR‐195, and miR‐466) and three protein‐coding genes (ITGA2, RAD18, and UBE3C). LINC00355 plays a role in regulating various biological processes such as cell cycle progression, proliferation, apoptosis, epithelial‐mesenchymal transition, invasion, and metastasis of cancer cells. It is involved in the regulation of the Wnt/β‐catenin signaling pathway and p53 signaling pathway. Upregulation of LINC00355 has been identified as a high‐risk factor in cancer patients and its increased expression is associated with poorer overall survival, recurrence‐free survival, and disease‐free survival. LINC00355 upregulation has been linked to several unfavorable clinical characteristics, including advanced tumor node metastasis and World Health Organization stages, reduced Karnofsky Performance Scale scores, increased tumor size, greater depth of invasion, and more extensive lymph node metastasis. LINC00355 induces chemotherapy resistance in cancer cells by regulating five downstream genes, namely HMGA2, ABCB1, ITGA2, WNT10B, and CCNE1 genes. In summary, LINC00355 is a potential oncogene with great potential as a diagnostic marker and therapeutic target for cancer.
LRRC59 serves as a novel biomarker for predicting the progression and prognosis of bladder cancer
Authors: Zhang Peng , Xie Xiaodu , Li Chunming , Zhang Chaohua , Liang Peihe 
Published: September 2023

AbstractBackgroundLeucine‐rich repeat‐containing protein 59 (LRRC59) is an endoplasmic reticulum membrane protein involved in various cancers, but its role in bladder cancer (BC) has not been reported. The aim of the present study was to investigate the role of LRRC59 protein in BC progression and prognosis.MethodsThe expression profile and clinical significance were retrieved from BC patients in the Cancer Genome Atlas database. The methylation status of LRRC59 was analyzed by UALCAN and MethSurv databases. Potential signaling pathways and biological functions were explored by functional enrichment analysis. Immunocyte infiltration was evaluated by CIBERSORT analysis. The prognostic value of LRRC59 was evaluated by Kaplan–Meier and Cox regression analyses. Overall survival (OS) was predicted by the nomogram plot established in this study. LRRC59 expression in 10 pairs BC and adjacent noncancerous tissues were analyzed by immunohistochemistry (IHC). Cell proliferation, migration, and invasion were detected by CCK8, colony formation assay, transwell assay, and cell scratch assay, respectively. Proteins related to epithelial–mesenchymal transition and apoptosis were detected by western blot.ResultsLRRC59 overexpression significantly decreased OS, disease‐specific survival, and progress‐free interval of BC patients. LRRC59 was a prognostic marker for OS and its hypomethylation status signified a poor prognosis. LRRC59 overexpression was correlated with infiltration of resting memory CD4 T cells, memory activated CD4 T cells, resting NK cells, macrophages M0, M1, M2, and neutrophils. IHC showed that the LRRC59 expression in BC tissue was significantly higher than that in adjacent noncancerous tissue. Knockdown of LRRC59 expression inhibited the proliferation of BC cells and reduced their migratory ability. Western blot showed that Snail and vimentin protein expressions decreased, while E‐cadherin expressions increased.ConclusionsLRRC59 expression can predict the outcome of BC independently and serve as a new biomarker for diagnosis.
Design, Synthesis of Novel 1,2,3‐Triazole Pendent Quinazolinones and Their Cytotoxicity against MCF‐7 Cell Line
Authors: Myakala Nagaraju , Kandula Kotaiah , Rayala Nagamani , Kuna Sateesh , Thumma Vishnu , Durga Bhavani Anagani Kanaka 
Published: September 2023

AbstractA library of 6‐(((1‐(substitutedphenyl)‐1H‐1,2,3‐triazol‐4‐yl)methyl) amino)‐3‐methylquinazolin‐4(3H)‐one analogues synthesized from Isatin precursor through a series of nitration, reduction, hydrolysis, cyclization and click reaction. The structures of compounds were characterized by spectral data including IR, 1H‐NMR, 13C NMR and Mass. The novel quinazolinone – 1,2,3‐triazoles were screened for their cytotoxicity against the human breast adenocarcinoma cell lines MCF‐7 by MTT assay. 4‐Isopropyl and 2‐bromo substituted analogues executed high activity against MCF‐7 cell line with IC50 value of 10.16±0.07 μM and 11.23±0.20 μM compared to the Doxorubicin whose IC50 value is 10.81±0.03 μM. The activity of remaining compounds is good to moderate. Further, the molecular docking studies against the crystal structure of Epidermal Growth Factor Receptor delivered the best binding energies and the interactions such as H‐bond and hydrophobic are inevitable. The predicted pharmacokinetic properties results showed that these compounds have more drug likeness properties.
Transforming growth factor β1 upregulates 6‐phosphofructo‐2‐kinase/fructose 2,6‐bisphosphatase‐4 expression in A549 and MCF‐10A cells
Authors: Altunok Tugba H. , Muchut Robertino J. , Iglesias Alberto A. , Yalcin Abdullah 
Published: September 2023

AbstractTransforming growth factor β1 (TGFβ1) induces a cellular process known as epithelial–mesenchymal transition (EMT) associated with metabolic reprogramming, including enhanced glycolysis. Given the involvement of 6‐phosphofructo‐2‐kinase/fructose 2,6‐bisphosphatase (PFKFB) enzymes in glycolysis, we aimed to investigate whether TGFβ1 regulates expressions of PFKFB genes and if PFKFBs are required for TGFβ1‐driven phenotypes. A549 and MCF‐10A cell lines were used as TGFβ1‐driven EMT models. Messenger RNA expressions of PFKFB and EMT genes were determined by real‐time quantitative polymerase chain reaction. A small interfering RNA approach was used to deplete PFKFB4 expression. A Matrigel invasion assay was conducted to assess the effect of PFKFB4 silencing on the TGFβ1‐enhanced invasion of A549 cells. F2,6BP levels were analyzed using an enzyme‐coupled assay. Glucose and lactate concentrations were determined using colorimetric assays. TGFβ1 robustly induced expression of the fourth isoform of PFKFBs, PFKFB4, in both cell lines. PFKFB4 depletion partially inhibits mesenchymal transdifferentiation caused by TGFβ1 in A549 cells, as assessed by microscopy. Inductions of Snail in MCF‐10A cells and Fibronectin in A549 cells and repressions of E‐cadherin in both cell lines by TGFβ1 are attenuated by PFKFB4 silencing. PFKFB4 silencing reduces F2,6BP and glycolytic activity, although TGFβ1 alone does not affect these parameters. Finally, PFKFB4 depletion suppresses the TGFβ1‐driven invasion of A549 cells through Matrigel. Presented data suggest that TGFβ1 induces the expression of PFKFB4 in A549 and MCF‐10 cells, and PFKFB4 may be required for TGFβ1‐driven phenotypes such as EMT and invasion in these models.
Coexistence of cerebral venous thrombosis and dural arteriovenous fistula in an adolescent: A case report
Authors: Paudyal Man Bahadur , Bhattarai Madhur , Mehta Neha , Gautam Niraj , Baral Bikas , Sharma Niraj Kumar , Basnet Rashika , Ghimire Bardan 
Published: September 2023

Key Clinical MessageClinicians should consider central venous thrombosis (CVT) as a differential diagnosis in young adolescents with persistent headaches. It is essential to assess for concurrent CVT and dural arteriovenous fistula (DAVF), particularly in those with a history of CVT.AbstractCerebral venous thrombosis (CVT) and dural arteriovenous fistula (DAVF) are uncommon vascular disorders with diverse clinical presentations. The coexistence of CVT and DAVF is a rare but important association that may impact the management and prognosis of affected patients. Prothrombotic conditions generally ranging from acquired to genetic, oral contraceptives, malignancy, puerperium, infection, and head injury are the common risk factors for cerebral venous thrombosis. Here, we present a case of 18 years males who developed recurrent cerebral thrombosis on the background of the presence of an arteriovenous fistula.
Double Hydroamination of Terminal Alkynes over Beta Zeolite‐Supported CuO Catalyst
Authors: Sai Gajula Krishna , Kumar Macharla Arun , Amrutham Vasu , Rohini Yennamaneni Divya , Boosa Murali , Madasu Ramulamma , Kumar Chelukalapally Anil , Nama Narender 
Published: September 2023

AbstractAn effective and novel heterogeneous beta zeolite supported CuO (Cuβ) catalyzed double hydroamination of alkynes for the synthesis of 2,3‐dihydroquinazolin‐4(1H)‐ones has been achieved in a solvent‐free condition. Cuβ zeolite was synthesized by impregnation method. The synthesized Cuβ was characterized by X‐ray diffraction, Fourier transformation infrared spectroscopy, transmission electron microscopy, scanning electron microscopy, and X‐ray photoelectron spectroscopy. Pure Hβ (18 % yield) or Nano CuO (44 % yield) displayed poor catalytic activity, whereas 1 % Cuβ (96 % yield) showed higher catalytic activity for double hydroamination of alkynes due to the synergistic effect of CuO and Hβ zeolite in the reaction. The new protocol offers an appealing approach to a number of di‐substituted quinazolinones with decent to excellent yields. The catalyst has been successfully recycled for up to 5 consecutive cycles.
Zinc Tetrapyrrole Coordinated to Imidazole Functionalized Tetracyanobutadiene or Cyclohexa‐2,5‐diene‐1,4‐diylidene‐expanded‐tetracyanobutadiene Conjugates: Dark vs. Light‐Induced Electron Transfer
Authors: Guragain Manan , Pinjari Dilip , Misra Rajneesh , D'Souza Francis 
Published: September 2023

AbstractUsing the popular metal‐ligand axial coordination self‐assembly approach, donor‐acceptor conjugates have been constructed using zinc tetrapyrroles (porphyrin (ZnP), phthalocyanine (ZnPc), and naphthalocyanine (ZnNc)) as electron donors and imidazole functionalized tetracyanobutadiene (Im‐TCBD) and cyclohexa‐2,5‐diene‐1,4‐diylidene‐expanded‐tetracyanobutadiene (Im‐DCNQ) as electron acceptors. The newly formed donor‐acceptor conjugates were fully characterized by a suite of physicochemical methods, including absorption and emission, electrochemistry, and computational methods. The measured binding constants for the 1 : 1 complexes were in the order of 104–105 M−1 in o‐dichlorobenzene. Free‐energy calculations and the energy level diagrams revealed the high exergonicity for the excited state electron transfer reactions. However, in the case of the ZnNc:Im‐DCNQ complex, owing to the facile oxidation of ZnNc and facile reduction of Im‐DCNQ, slow electron transfer was witnessed in the dark without the aid of light. Systematic transient pump‐probe studies were performed to secure evidence of excited state charge separation and gather their kinetic parameters. The rate of charge separation was as high as 1011 s−1 suggesting efficient processes. These findings show that the present self‐assembly approach could be utilized to build donor‐acceptor constructs with powerful electron acceptors, TCBD and DCNQ, to witness ground and excited state charge transfer, fundamental events required in energy harvesting, and building optoelectronic devices.
Mediation analysis of activities of daily living and kinesiophobia in association between cardiac function and health status of patients with chronic heart failure
Authors: Yifan Tang , Yanling Huang , Weiyun Wang , Xiaolin Hu , Zejuan Gu , Rong Wang , Chunhong Gao 
Published: September 2023

AbstractAimsTo explore the mediational effect of activities of daily living (ADL) and kinesiophobia on the cardiac function and health status of patients with chronic heart failure (CHF).MethodsFrom October 2021 to January 2022, a total of 244 CHF patients treated in the Department of Cardiology of general hospitals were recruited by the convenience sampling method. They were investigated with the Tampa Scale for Kinesiophobia Heart (TSK‐SV Heart), the Barthel index for assessing ADL, and the EuroQol five‐dimensional questionnaire (EQ‐5D) for assessing the health status.ResultsThe cardiac function and kinesiophobia of CHF patients were both negatively correlated with their health status (r = −.390 and −0.410, respectively, both p < .01). Besides, the ADL of CHF patients was positively correlated with the health status (r = .320, p < .01). The cardiac function of CHF patients was negatively correlated with the ADL (r = −.412, p < .01), but positively correlated with kinesiophobia (r = .180, p < .01). The mediation proportion of ADL plus kinesiophobia between the cardiac function and health status of CHF patients was 43.48%. Both ADL and kinesiophobia partially mediated the effect of cardiac function on health status in CHF patients, but their mediational effects showed no significant difference (p = .777).ConclusionBoth ADL and kinesiophobia exert obvious mediational effects between cardiac function and health status in CHF patients. Individualized cardiac rehabilitation (CR) programs based on the cardiac function, ADL and kinesiophobia of CHF patients may contribute to reduce the medical burden and improve the well‐being of affected people.
Oncology hospitalist impact on hospice utilization
Authors: Prsic Elizabeth , Morris Jensa C. , Adelson Kerin B. , Parker Nathaniel A. , Gombos Erin A. , Kottarathara Mathew J. , Novosel Madison , Castillo Lawrence , Gould Rothberg Bonnie E. 
Published: September 2023

AbstractBackgroundUnplanned hospitalizations among patients with advanced cancer are often sentinel events prompting goals of care discussions and hospice transitions. Late referrals to hospice, especially those at the end of life, are associated with decreased quality of life and higher total health care costs. Inpatient management of patients with solid tumor malignancies is increasingly shifting from oncologists to oncology hospitalists. However, little is known about the impact of oncology hospitalists on the timing of transition to hospice.ObjectiveTo compare hospice discharge rate and time to hospice discharge on an inpatient oncology service led by internal medicine‐trained hospitalists and a service led by oncologists.MethodsAt Smilow Cancer Hospital, internal medicine‐trained hospitalists were integrated into one of two inpatient medical oncology services allowing comparison between the new, hospitalist‐led service (HS) and the traditional, oncologist‐led service (TS). Discharges from July 26, 2021, through January 31, 2022, were identified from the electronic medical record. The odds ratio for discharge disposition by team was calculated by logistic regression using a multinomial distribution. Adjusted length of stay before discharge was assessed using multivariable linear regression.ResultsThe HS discharged 47/400 (11.8%) patients to inpatient hospice, whereas the TS service discharged 18/313 (5.8%), yielding an adjusted odds ratio of 1.94 (95% CI, 1.07–3.51; p = .03). Adjusted average length of stay before inpatient hospice disposition was 6.83 days (95% CI, 4.22–11.06) for the HS and 16.29 days (95% CI, 7.73–34.29) for the TS (p = .003).ConclusionsOncology hospitalists improve hospice utilization and time to inpatient hospice referral on an inpatient medical oncology service.Plain Language Summary

Patients with advanced cancer are often admitted to the hospital near the end of life. These patients generally have a poor chance of long‐term survival and may prefer comfort‐focused care with hospice.
In this study, oncology hospitalists discharged a higher proportion of patients to inpatient hospice with less time spent in the hospital before discharge.
Acceptability, feasibility and preliminary efficacy of a compassion‐based cognitive behavioural intervention for low self‐esteem in sexual minority young adults
Authors: Bridge Livia , Langford Katie , McMullen Katrina , Rai Lovedeep , Smith Patrick , Rimes Katharine A. 
Published: September 2023

AbstractLow self‐esteem can impair daily functioning and is a risk or maintenance factor for several mental health problems. Sexual minority young adults, for example, those identifying as lesbian, gay or bisexual, on average have lower self‐esteem than their heterosexual peers. Compassion‐based interventions for low self‐esteem might be especially beneficial for sexual minority young adults, whose higher levels of shame and self‐criticism are likely to be contributing to maintenance of their low self‐esteem. This study explored the acceptability, feasibility and preliminary efficacy of a new compassion‐based cognitive behavioural intervention for low self‐esteem tailored to sexual minority young adults. Participants were a community sample of 24 sexual minority young adults aged 16–24 experiencing clinically significant low self‐esteem. An uncontrolled pre‐/post‐design was used with qualitative feedback and quantitative outcomes. Self‐report standardised measures were completed at baseline, pre‐intervention, intervention mid‐point, post‐intervention and 2‐month follow up. Preliminary efficacy was examined through post‐intervention changes in self‐esteem, functioning, anxiety and depression. Potential mechanisms of change were examined through changes in self‐compassion, self‐criticism and unhelpful coping responses to minority stress. Results showed good acceptability and feasibility and preliminary evidence of intervention efficacy for improving self‐esteem, functioning, depression and anxiety. There was evidence for improvement in self‐compassion and reduction in self‐criticism, and these constructs could be investigated in future studies as treatment mediators. Randomised controlled studies are needed to further examine efficacy of the intervention.