bluuebunny/crossref_metadata_2025_split huggingface dataset:
Nanosilicate-functionalized nanofibrous membrane facilitated periodontal regeneration potential by harnessing periodontal ligament cell-mediated osteogenesis and immunomodulation
Authors: Xu Xiongcheng , Xiao Long , Xu Yanmei , Xiao Nianqi , Jin Weiqiu , Chen Yuling , Li Yanfen , Luo Kai 
Published: January 2023

Abstract
Background: Although various new biomaterials have enriched the methods for periodontal regeneration, their efficacy is still controversial, and the regeneration of damaged support tissue in the periodontium remains challenging. Laponite (LAP) nanosilicate is a layered two-dimensionalnanoscale, ultrathin nanomaterial with a unique structure and brilliant biocompatibility and bioactivity. Incorporation of LAP nanosilicate into polycaprolactone (PCL/LAP) provided cumulative effects on osteogenesis, angiogenesis and osteoclastogenesis during bone regeneration.
Methods:This study aimed to investigate the effects of nanosilicate-incorporated PCL (PCL/LAP) nanofibrous membranes on periodontal ligament cells (PDLCs) in vitro and periodontal regeneration in vivo. A PCL/LAP nanofibrous membrane was fabricated by an electrospinning method. The morphology and mechanical property of PCL/LAP nanofibrous membrane were characterized by scanning electron microscopy (SEM), energy dispersive spectrum of X-ray (EDS) and tensile test. The proliferationand osteogenic differentiation of PDLCs on the PCL/LAP nanofibrous membrane were determined. A PDLCs and macrophage coculturesystem was used to explore the immunomodulatory effects of the PCL/LAP nanofibrous membrane. PCL/LAP nanofibrous membrane was implanted into rat calvarial and periodontal defects, and the regenerative potential was evaluated by microcomputed topography (micro-CT) and histological analysis.
Results: The PCL/LAP nanofibrous membrane showed good biocompatibility and bioactivity. It enhanced the proliferation and osteogenic differentiation of PDLCs. The PCL/LAP nanofibrous membrane also stimulated anti-inflammatory and pro-remodeling N2 neutrophil formation, regulated inflammatory responses and induced M2 macrophage polarization by orchestrating the immunomodulatory effects of PDLCs. The PCL/LAP nanofibrous membrane promoted rat calvarial defect repair and periodontal regeneration in vivo.
Conclusions: LAP nanosilicate-incorporated PCL membrane is capable of mediating osteogenesis and immunomodulation of PDLCs in vitro and acceleratingperiodontal regeneration in vivo. It could be a promising biomaterial for periodontal regeneration therapy.
PEGylated Nanoparticles Loaded with 2-Methoxyestradiol for the Treatment of Uterine Leiomyoma in a Patient-Derived Xenograft Mouse Model
Authors: Al-Enazy Sanaalarab , Kirschen Gregory W. , Vincent Kathleen , Yang Jinping , Saada Jamal , Shah Mansi , Motamedi Massoud , Salama Salama A. , Kilic Gokhan , Rytting Erik , Borahay Mostafa 
Published: January 2023

Abstract
Leiomyomas, the most common benign neoplasms of the female reproductive tract, currently have limited medical treatment options. Drugs targeting estrogen/progesterone signaling are used, but side effects and limited efficacy in many cases are major limitation of their clinical use. Previous studies from our laboratory and others demonstrated that 2-methoxyestradiol (2-ME) is promising treatment for uterine fibroids. However, its poor bioavailability and rapid degradation hinder its development for clinical use. The objective of this study is to evaluate the in vivo effect of biodegradable and biocompatible 2-ME-loaded PEGylated nanoparticles in a patient-derived leiomyoma xenograft mouse model. Diblock copolymer nanoparticles loaded with 2-ME were prepared by nanoprecipitation. Female 6-week age immunodeficient NOG (NOD/Shi-scid/IL-2Rγnull) mice were used. Estrogen-progesterone pellets were implanted subcutaneously. Five days later, patient-derived human fibroid tumors were xenografted bilaterally subcutaneously. Engrafted mice were treated with 2-ME-loaded or blank (control) PEGylated nanoparticles. Nanoparticles were injected intraperitoneally and after 28 days of treatment, tumor volume was measured by caliper following hair removal, and tumor were removed and weighed. Up to 99.1% encapsulation efficiency was achieved, and the in vitro release profile showed minimal burst release, thus confirming the high encapsulation efficiency. In vivo administration of the 2-ME-loaded nanoparticles led to 51% growth inhibition of xenografted tumors compared to controls (P < 0.01). Thus, 2-ME-loaded nanoparticles may represent a novel approach for the treatment of uterine fibroids.
Work-Related Musculoskeletal Symptoms Among Saudi Radiologists: A Cross-Sectional Multi-Centre Study
Authors: Alelyani Magbool , Gameraddin Moawia , Alkhushayl Abdullah M. , Altowaijri Aljoharah M. , Qashqari Maryam Ibrahim , Alzahrani Fahad Ali Ahmed , Gareeballah Awadia 
Published: January 2023

Abstract
Background
Musculoskeletal disorders are common health problems worldwide. Several factors cause these symptoms, including ergonomics and other individual considerations. Computer users are prone to repetitive strain injuries that increase the risk of developing musculoskeletal symptoms (MSS). Radiologists are susceptible to developing MSS because they work long hours analysing medical images on computers in an increasingly digitalised field. This study aimed to identify the prevalence of MSS among Saudi radiologists and the associated risk factors.
Methods
This cross-sectional study was conducted on November 12, 2021, on 814 Saudi radiologists from various regions in Saudi Arabia. It comprised a Nordic Musculoskeletal Questionnaire examination of MSS. The study's primary outcome was the presence of musculoskeletal complaints in any body region that limited participation in routine activities over the previous 12 months. The results were descriptively examined using binary logistic regression analysis to estimate the odds ratio of participants who had disabling musculoskeletal complaints in the previous 12 months. All university, public, and private radiologists received an online survey containing questions about work surroundings, workload (e.g., spent at a computer workstation), and demographic characteristics.
Results
The prevalence of musculoskeletal complaints among the radiologists was 87.7%. Most of the participants (82%) were younger than 40 years of age. Radiography and computed tomography were the most common imaging modalities that caused MSS (53.4% and 26.8%, respectively). The most common symptoms were neck pain (59.3%) and lower back pain (57.1%). After adjustment, age, years of experience, and part-time employment were significantly associated with increased MSS (OR = .219, 95% CI = .057–.836; OR = .235, 95% CI = 087–.634; and OR = 2.673, 95% CI = 1.434–4.981, respectively). Females were more likely to report MSS than males (OR = 2.12, 95% CI = 1.327–3.377).
Conclusions
MSS are common among Saudi radiologists, with neck pain and lower back pain being the most frequently reported symptoms. Gender, age, years of experience, type of imaging modality, and employment status were the most common associated risk factors for developing MSS. These findings are vital for the development of interventional plans to reduce the prevalence of musculoskeletal complaints in clinical radiologists.
EDB-FN targeted probes for the surgical navigation, radionuclide imaging and therapy of thyroid cancer
Authors: Li Ruping , He Huihui , Li Xinling , Zheng Xiaobin , Li Zhijian , Zhang Hu , Ye Jiacong , Zhang Weiguang , Yu Chunjing , Feng Guokai , Fan Wei 
Published: January 2023

Abstract
Purpose
Extradomain B of fibronectin (EDB-FN) is a promising diagnostic and therapeutic biomarker for thyroid cancer (TC). Here, we identified a high-affinity EDB-FN targeted peptide named EDBp, and developed three EDBp-based probes, Cy5-EDBp, [18F]-EDBp and [177Lu]-EDBp the surgical navigation, radionuclide imaging and therapy of TC.
Methods
Based on the previously identified EDB-FN targeted peptide ZD2, the optimized EDB-FN targeted peptide EDBp was identified by using the alanine scan strategy. Three EDBp-based probes, Cy5-EDBp, [18F]-EDBp and [177Lu]-EDBp, were developed for fluorescence imaging, positron emission tomography (PET) imaging and radiotherapy in TC tumor-bearing mice, respectively. Additionally, [18F]-EDBp was evaluated in two TC patients.
Results
The binding affinity of EDBp to the EDB fragment protein (Kd = 14.4 ± 1.4 nM, n = 3) was approximately 336-fold greater than that of the ZD2 (Kd = 4839.7 ± 361.7 nM, n = 3). Fluorescence imaging with Cy5-EDBp facilitated the complete removal of TC tumors. [18F]-EDBp PET imaging clearly delineated TC tumors, with high tumor uptake (16.43 ± 1.008%ID/g, n = 6, at 1 h postinjection). Radiotherapy with [177Lu]-EDBp inhibited tumor growth and prolonged survival in TC tumor-bearing mice (survival time of different treatment groups: Saline vs. EDBp vs. ABRAXANE vs. [177Lu]-EDBp = 8 d vs. 8 d vs. 11.67 d vs. 22.33 d, ***p < 0.001). Importantly, the first-in-human evaluation of [18F]-EDBp demonstrated that it had specific targeting properties (SUVmax value of 3.6) and safety.
Conclusion
Cy5-EDBp, [18F]-EDBp and [177Lu]-EDBp are promising candidates for the surgical navigation, radionuclide imaging and radionuclide therapy of TC, respectively.
Temporal relationships between BMI and obesity-related predictors of cardiometabolic and breast cancer risk in a longitudinal cohort
Authors: Xu Bin , LV Liang , Chen Xin , Li Xingyue , Zhao Xunying , Yang Huifang , Feng Wanting , Jiang Xia , Li Jiayuan 
Published: January 2023

Abstract
Background: Prospective inter-relationships among biomarkers were unexplored, which may provide mechanistic insights into diseases. We investigated the longitudinal associations of BMI change with trajectories of biomarkers related to cardiometabolic or breast cancer risk.
Methods: A longitudinal study was conducted among 400 healthy women between 2019 to 2021. Cross‑lagged path analysis was used to examine the temporal relationships among BMI, cardiometabolic risk score (CRS), and obesity‑related proteins score (OPS) of breast cancer. Linear mixed-effect models were applied to investigate associations of time-varying BMI with biomarker-based risk score trajectories.
Results: Baseline BMI was associated with subsequent change of both cardiometabolic (P=0.06) and breast cancer predictors (P=0.03), and baseline CRS were positively associated with OPS change (P<0.001) but not vice versa. After fully adjustment of confounders, we found a 0.058 (95%CI= 0.009-0.107, P=0.020) units increase of CRS and a 1.021 (95%CI= 0.041-1.995, P=0.040) units increase of OPS as BMI increased 1 kg/m2 per year in postmenopausal women. OPS increased 0.784 (95%CI= 0.053-1.512, P=0.035) units as CRS increased 1 unit per year. However, among premenopausal women, BMI only significantly affected CRS (β=0.057, 95%CI=0.007 to 0.107, P=0.025). No significant change of OPS with time-varying CRS was found.
Conclusions: Higher increase rates of BMI were associated with worse trajectories of biomarker-based risk of cardiometabolic and breast cancer. The longitudinal impact of CRS on OPS is unidirectional.
Impacts: Recommendations such as weight control for the reduction of cardiometabolic risk factors may benefit breast cancer prevention, especially in postmenopausal women.
Lived experiences and perspectives of patients with type 2 diabetes on poor glycaemic control in Kinshasa, Democratic Republic of the Congo: a qualitative study
Authors: Lubaki Jean-Pierre Fina , Francis Joel Msafiri , Omole Olufemi Babatunde 
Published: January 2023

Abstract

Background
Glycaemic control is a significant problem in diabetes care in sub-Saharan Africa. In the Democratic Republic of the Congo, factors associated with glycaemic control are not fully known as previous studies rarely explored patients’ perspectives and lived experiences. This study describes the lived experiences and perspectives of patients with type 2 diabetes regarding glycaemic control in Kinshasa, Democratic Republic of the Congo.
Methods
This study was a qualitative study conducted in 7 health centres in 7 health districts (out of 24) in Kinshasa. Purposively selected patients with type 2 were invited to participate in in-depth interviews to explore their lived experience on diabetes treatment and glycaemic control. All interviews were audio recorded, transcribed verbatim and translated into English by a language expert. Data coding and thematic analysis were performed using the MAXQDA 2022.
Results
Twenty-three participants were included in the study. Six themes emerged as explanations for glycaemic control in Kinshasa. Three of these themes were related to poor glycaemic control: poor self-care, poor clinical care in primary health care facilities, and limited sociodemographic and financial support. Three additional themes emerged for good glycaemic control: motivation and commitment to treatment, individualized and available care, and ability to perform the change for glycaemic control.
Conclusions
Patients’ perspectives and lived experiences of diabetes care and glycaemic control in Kinshasa transcend personal, health system and psychosocial aspects of care. Efforts to strengthen diabetes care in this setting need to focus on promoting self-care, training healthcare providers on an evidence-based clinical approach, and providing psychosocial support that motivates patients to commit to care. The implementation of universal health coverage at the primary care level would ensure accessibility to diabetes care for socioeconomically deprived patients.
Genome-wide identification and expression pattern analysis of R2R3-MYB transcription factor gene family involved in puerarin biosynthesis and response to hormone in Puararia lobata var. thomsonii
Authors: Wu Zhengdan , Zeng Wendan , Li Changfu , Wang Jihua , Shang Xiaohong , Xiao Liang , Cao Sheng , Zhang Yansheng , Xu Shiqiang , Yan Huabing 
Published: January 2023

Abstract
Background
R2R3-MYB transcription factors regulate secondary metabolism, stress responses and development in various plants. Puerarin is a bioactive ingredient and most abundant secondary metabolite isolated from Puararia lobata. The biosynthesis of puerarin proceeds via the phenylpropanoid pathway and isoflavonoids pathway, in which 9 key enzymes are involved. The expression of these structural genes is under control of specific PtR2R3-MYB genes in different plant tissues. However, how PtR2R3-MYB genes regulates structural genes in puerarin biosynthesis remains elusive. This study mined the PtR2R3-MYB genes involved in puerarin biosynthesis and response to hormone in Puararia lobata var. thomsonii.
Results
A total of 209 PtR2R3-MYB proteins were identified, in which classified into 35 subgroups based on the phylogenetic topology and the classification of the R2R3-MYB superfamily in Arabidopsis thaliana. Furtherly physical and chemical characteristics, gene structure, and conserved motif analysis were also used to further analyze PtR2R3-MYBs. Combining puerarin content and RNA-seq data, speculated on the regulated puerarin biosynthesis of PtR2R3-MYB genes and structural genes, thus 21 PtR2R3-MYB genes and 25 structural genes were selected for validation gene expression and further explore its response to MeJA and GSH treatment by using qRT-PCR analysis technique. Correlation analysis and cis-acting element analysis revealed that 6 PtR2R3-MYB genes (PtMYB039, PtMYB057, PtMYB080, PtMYB109, PtMYB115 and PtMYB138) and 7 structural genes (PtHID2, PtHID9, PtIFS3, PtUGT069, PtUGT188, PtUGT286 and PtUGT297) were directly or indirectly regulation of puerarin biosynthesis in FG11. It is worth noting that after MeJA and GSH treatment for 12–24 hours, the expression changes of most candidate genes were consistent with the correlation of puerarin biosynthesis, which also shows that MeJA and GSH have the potential to mediate puerarin biosynthesis by regulating gene expression in FG11.
Conclusions
Overall, this study provides a comprehensive understanding of the PtR2R3-MYB and will paves the way to reveal the transcriptional regulation of puerarin biosynthesis and response to phytohormone of PtR2R3-MYB genes in Puararia lobata var. thomsonii.
Airway collapse as the predominant clinical feature following remimazolam-induced anaphylaxis: a case report
Authors: Hu Xiawei , Tang Yaning , Fang Xiangming 
Published: January 2023

Abstract
Background
remimazolam is an ultra-short-acting intravenous benzodiazepine, which has been used as sedative/anesthetic in procedural sedation and anesthesia. Although peri-operative anaphylaxis due to remimazolam has been reported recently, the spectrum of the allergic reactions is still not fully known.
Case presentation:
we describe a case of anaphylaxis following remimazolam administration in a male patient undergoing colonoscopy under procedural sedation. The patient presented complex clinical signs including airway changes, skin symptoms, gastrointestinal manifestations and hemodynamic fluctuations. Different from other reported cases, respiratory compromise was the initial and main clinical feature of remimiazolam-induced anaphylaxis.
Conclusions
remimazolam-induced anaphylaxis has a rapid onset and complex clinical features. This case reminds anesthesiologists should be particularly alert to the unknown adverse reactions of new anesthetics.
De Garengeot Hernia, an acute appendicitis in the right femoral hernia canal, and successful management with transabdominal closure and appendectomy: A Case Report
Authors: YU POCHUAN , Wang Ling-Ting , Chang Chun-Yu , Tsai Yao-Chou , Chong Kian-Hwee 
Published: January 2023

Abstract
Incarceration of the appendix within a femoral hernia is a rare condition of abdominal wall hernia. We report a case of a 56-year-old female whose appendix was trapped in the right femoral canal. There are few reports in the literature on entrapment of the appendix within a femoral hernia. The management of this condition includes antibiotics, drainage appendectomy, hernioplasty and mesh repair.
Treatment of osteoporosis using a selective androgen receptor modulator ostarine in an orchiectomized rat model
Authors: Böker Kai O. , Komrakova Marina , Fahrendorff Linda , Spelsberg Bastian Ranjith , Hoffmann Daniel Bernd , Schilling Arndt F. , Lehmann Wolfgang , Taudien Stefan , Sehmisch Stephan 
Published: January 2023

Abstract
Purpose:
The selective androgen receptor modulator ostarine has been shown to have advantageous effects on skeletal tissue properties, reducing muscle wasting and improving physical function in males. However, data on effects in male osteoporosis remain limited. In this study, the effects of ostarine on osteoporotic bone were evaluated in a rat model of male osteoporosis and compared with those of testosterone treatments.
Methods: Eight-month-old male Sprague-Dawley rats were orchiectomized (Orx) and grouped (n=15/group):
1) Orx, 2) Ostarine Therapy, 3) Test. Therapy, 4) Ostarine Prophylaxe and 5) Test. Proph. Fifteen rats were left non-orchiectomized (Non Orx). Proph. treatments were begun directly after orchiectomy and continued for 18 weeks, whereas Therapy treatments were initiated 12 weeks after Orx. Ostarine and Test. were applied orally at daily doses of 0.4 and 50 mg/kg body weight, respectively. The lumbar vertebral bodies and femora were analyzed via biomechanical, micro-CT, ashing, and gene expression analyses.
Results: Ostarine Proph. showed positive effects in preventing osteoporotic changes in cortical and trabecular bone; biomechanical parameters were not affected; prostate weight was increased. Ostarine Therapy increased solely the cortical density of the femur; other bone parameters remained unaffected. Test. Proph. positively influenced cortical density in femur; Test. Therapy did not change any bony parameters.
Conclusion: Ostarine Proph. could be further investigated as a preventative treatment for male osteoporosis, but an androgenic effect on the prostate comparable to testosterone should be taken into consideration. Combination therapies with other anti-osteoporosis agents should be considered in future studies to strengthen the selective effects on the bone tissue.
Severe phenotype of a heterozygous with variant on FGFR3 in the second trimester: a case report
Authors: Chen Shujun , Dong Hongmei , Luo Yong , Zhang Yingpin , Li Pan 
Published: January 2023

Abstract
Background

Achondroplasia is a congenital skeletal system malformation caused by missense mutation of FGFR3 gene with an incidence of 1 per 20,000–30,000 newborns, which is an autosomal dominant inheritance disease. Despite similar imaging features, the homozygous achondroplasia is absolutely lethal due to thoracic stenosis, whereas heterozygous achondroplasia does not lead to fetal death.

Case presentation

A fetus with progressive rhizomelic short limbs and overt narrow chest was detected by prenatal ultrasound in the second trimester. Gene sequencing results of amniotic fluid sample indicated a rare missense variant NM_000142.4: c.1123G > T(p.Gly375Cys), leading to a glusate-to-cysteine substitution. Re-sequencing confirmed that it was a heterozygous mutation, and thoracic stenosis was then confirmed in the corpse by radiological examination.

Conclusions

We identified a wild heterozygous variant of the FGFR3 gene as the rare pathogenic mutation of severe achondroplasia in a fetus. Heterozygous variants of p.Gly375Cys may have a severe phenotype similar to homozygote. It’s crucial to combine prenatal ultrasound with genetic examination to differentiate heterozygous from homozygous achondroplasia. The p.Gly375Cys mutation of FGFR3 gene may serve as a vital target for the diagnosis of severe achondroplasia.
The soil microbiomes of forest ecosystems in Kenya: their diversity and environmental drivers
Authors: Onyango Lorine Akinyi , Ngonga Florence Atieno , Karanja Edward Nderitu , Ochieng Josiah Kuja , Boga Hamadi Iddi , Cowan Don A , Mwangi Kennedy Wanjau , Maghenda Marianne Wughanga , Lebre Pedro Bixirao Neto Marinho , Kambura Anne Kelly 
Published: January 2023

Abstract
Soil microbiomes in forest ecosystems act as both nutrient sources and sinks through a range of processes including organic matter decomposition, nutrient cycling, and humic compound incorporation into the soil. Most forest soil microbial diversity studies have been performed in the northern hemisphere, and very little has been done in forests within African continent. This study examined the composition, diversity and distribution of prokaryotes in Kenyan forests top soils using amplicon sequencing of V4-V5 hypervariable region of the 16S rRNA gene. Additionally, soil physicochemical characteristics were measured to identify abiotic drivers of prokaryotic distribution. Different forest soils were found to have statistically distinct microbiome compositions, with Proteobacteria and Crenarchaeota taxa being the most differentially abundant across regions within bacterial and archaeal phyla, respectively. Key bacterial community drivers included pH, Ca, K, Fe, and total N while archaeal diversity was shaped by Na, pH, Ca, total P and total N. To contextualize the prokaryote diversity of Kenyan forest soils on a global scale, the sample set was compared to amplicon data obtained from forest biomes across the globe; displaying them to harbor distinct microbiomes with an over-representation of uncultured taxa such as TK-10 and Ellin6067 genera.