bluuebunny/crossref_metadata_2025_split huggingface dataset:
Network Pharmacology and Molecular Docking Analysis on Molecular Targets and
Mechanisms of Fei Jin Sheng Formula in the Treatment of Lung Cancer
Authors: Zhang Yun-Chao , Gao Wen-Cang , Chen Wei-Jian , Pang De-Xiang , Mo Da-Yu , Yang Min 
Published: May 2023

Background:
Fei Jin Sheng Formula (FJSF) is widely used in clinical treatment of lung cancer. But
the underlying active ingredients and mechanisms are unclear.

Objective:
To investigate the active components and functional mechanisms of FJSF in treating lung cancer
using a network pharmacology approach and molecular docking combined with vitro experiments.

Methods:
Based on the TCMSP and related literature, the chemical components of related herbs in FJSF were
collected. The active components of FJSF were screened by ADME parameters, and the targets were predicted
by the Swiss Target Prediction database. The "drug-active ingredient-target" network was constructed by Cytoscape.
Disease-related targets of lung cancer were acquired from GeneCards, OMIM, and TTD databases. Then
drug-disease intersection target genes were obtained through the Venn tool. GO analysis and KEGG pathway
enrichment analysis were performed via the Metascape database. Cytoscape was used to construct a PPI network
and perform topological analysis. Kaplan-Meier Plotter was used to analyze the relationship between
DVL2 and the prognosis of lung cancer patients. xCell method was used to estimate the relationship between
DVL2 and immune cell infiltration in lung cancer. Molecular docking was performed by AutoDockTools-1.5.6.
The results were verified by experiments in vitro.

Results:
FJSF contained 272 active ingredients and 52 potential targets for lung cancer. GO enrichment analysis is
mainly related to cell migration and movement, lipid metabolism, and protein kinase activity. KEGG pathway enrichment
analysis mainly involves PI3K-Akt, TNF, HIF-1, and other pathways. Molecular docking shows that the
compound Xambioona, quercetin and methyl palmitate in FJSF has a strong binding ability with NTRK1, APC,
and DVL2. Analysis of the data in UCSC to analyze the expression of DVL2 in lung cancer shows that DVL2
was overexpressed in lung adenocarcinoma tissues. Kaplan-Meier analysis shows that the higher DVL2 expression
in lung cancer patients was associated with poorer overall survival and poorer survival in stage I patients. It
was negatively correlated with the infiltration of various immune cells in the lung cancer microenvironment.
Vitro Experiment showed that Methyl Palmitate (MP) can inhibit the proliferation, migration, and invasion of
lung cancer cells, and its mechanism of action may be to downregulate the expression of DVL2.

Conclusion:
FJSF may play a role in inhibiting the occurrence and development of lung cancer by downregulating
the expression of DVL2 in A549 cells through its active ingredient Methyl Palmitate. These results provide
scientific evidence for further investigations into the role of FJSF and Methyl Palmitate in the treatment of
lung cancer.
Kechuanning Gel Plaster Exerts Anti-inflammatory and Immunomodulatory Effects on Ovalbumin-induced Asthma Model Rats via ERK Pathway
Authors: Xie Miaomiao , Liu Tingting , Yin Jie , Liu Jing , Yang Liu , Li Ting , Xia Chen , Fan Yanbo 
Published: May 2023

Background:
We aimed to evaluate the therapeutic effects of Kechuanning gel plaster
on ovalbumin (OVA)-induced rat model of asthma.

Methods:
Rats were injected with OVA to induce asthma, and Kechuanning gel plaster was administered
after the OVA challenge. The immune cell counts in the bronchial alveolar lavage fluid
(BALF) were calculated after Kechuanning gel plaster administration. The levels of immune factors
in BALF and serum OVA-specific IgE levels were analyzed. Western blot analysis and immunohistochemistry
were carried out to analyze the following proteins: C-FOS, C-JUN, RAS p21
protein activator 1 (RASA1), matrix metalloproteinase 9 (MMP9), RAF1, p-MEK1, tissue inhibitor
of metalloproteinase-1 (TIMP1), and p-extracellular signal-regulated kinase 1 (ERK1).

Results:
Administration of Kechuanning gel plaster led to decreased immune cell counts, inflammatory
cytokines (interleukin (IL)-1β, IL13, and IL17), and OVA-specific IgE expression. Compared
to the normal group, the C-FOS, C-JUN, RASA1, MMP9, RAF1, MEK1, TIMP1, and p-
ERK1 expressions in the model group were significantly increased, whereas Kechuanning gel plaster
administration decreased C-JUN, MMP9, TIMP1, RAF1, MEK1, p-ERK1, C-FOS, and RASA1
protein levels.

Conclusion:
Kechuanning gel plaster exerted its therapeutic effects on OVA-induced asthma model
rats through the ERK signaling pathway. Kechuanning gel plaster could be considered as a
potential alternative therapeutic agent for the management of asthma.
Antiviral Flavonoids: A Natural Scaffold with Prospects as Phytomedicines
against SARS-CoV2
Authors: Saha Chiranjeet , Naskar Roumi , Chakraborty Sandipan 
Published: May 2023

Abstract:
Flavonoids are vital candidates to fight against a wide range of pathogenic microbial infections. Due to their therapeutic potential, many flavonoids from the herbs of traditional medicine systems are now being evaluated as lead compounds to develop potential antimicrobial hits. The emergence of SARS-CoV-2 caused one of the deadliest pandemics that has ever been known to mankind. To date, more than 600 million confirmed cases of SARS-CoV2 infection have been reported worldwide. Situations are worse due to the unavailability of therapeutics to combat the viral disease. Thus, there is an urgent need to develop drugs against SARS-CoV2 and its emerging variants. Here, we have carried out a detailed mechanistic analysis of the antiviral efficacy of flavonoids in terms of their potential targets and structural feature required for exerting their antiviral activity. A catalog of various promising flavonoid compounds has been shown to elicit inhibitory effects against SARS-CoV and MERS-CoV proteases. However, they act in the high-micromolar regime. Thus a proper lead-optimization against the various proteases of SARS-CoV2 can lead to high-affinity SARS-CoV2 protease inhibitors. To enable lead optimization, a quantitative structure-activity relationship (QSAR) analysis has been developed for the flavonoids that have shown antiviral activity against viral proteases of SARS-CoV and MERS-CoV. High sequence similarities between coronavirus proteases enable the applicability of the developed QSAR to SARS-CoV2 proteases inhibitor screening. The detailed mechanistic analysis of the antiviral flavonoids and the developed QSAR models is a step forward toward the development of flavonoid-based therapeutics or supplements to fight against COVID-19.
Dual Specificity Phosphatase 3 (DUSP3) Knockdown Alleviates Acute
Myocardial Infarction Damage via Inhibiting Apoptosis and Inflammation
Authors: Yu Kun , Jiang Aixia , Zhao Caixia , Zhang Dongying 
Published: May 2023

Background:
Dual Specificity Phosphatase 3 (DUSP3) regulates the innate immune
response and is associated with ischemia/reperfusion (I/R). However, the precise function of
DUSP3 in acute myocardial infarction (AMI) remains to be established.

Methods:
In this study, the AMI model in vivo was established in mice by permanent left anterior
descending coronary artery (LAD) occlusion, and primary neonatal mouse cardiomyocytes were
treated with hypoxia for 12 hours to mimic AMI in vitro. Sh-DUSP3 and AAV9-sh-DUSP3 were
used to knock down the DUSP3 expression. LVEF%, LVFS%, SOD1, and HO-1 level, and TTC
staining were used to test the cardiac function. Flow cytometric analysis, Western blot, and
TUNEL staining were used to investigate the effect of DUSP3 knockdown on apoptosis. Moreover,
we detect inflammatory factors expression and oxidative stress by ELISA. Besides, we investigate
DUSP3 expression by RT-qPCR.

Results:
Our findings determined the role of DUSP3 in the progression of AMI. And demonstrated
that DUSP3 knockdown alleviated oxidative stress, inflammation, and apoptosis. In addition, our
results indicated that DUSP3 knockdown could regulate the expression of p-NF-κB, ICAM1, and
VCAM1.

Conclusion:
Our results demonstrated that the knockdown of DUSP3 could effectively alleviate
AMI symptoms and be mediated through the NF-κB signaling pathway.
Oxidative Biotransformation of Organophosphotioate Pesticides and Acetylcholinesterase Enzymatic Inhibition
Authors: Militani Isabela Aparecida , Mancini Daiana Teixeira , de Castro Alexandre Alves , Ramalho Teodorico Castro 
Published: May 2023

Introduction:
Pesticides have lethal properties, capable of controlling or eliminating a living
organism; they block the organisms' vital metabolic processes. They cause serious problems for human
health, as they are highly toxic. The most used pesticides that are considered toxic are known as organophosphothioates
(OP/P=S) in their commercialized form and organophosphates (OP/P=O) in their active
form. These compounds have been the subject of studies on their metabolism and toxicology. According
to research, these pesticides' toxicity is increased when oxidative metabolic desulfurization reactions occur,
with the P=S bond being transformed into a P=O bond. This toxicity is due to the ability of OP/P=O
species to inhibit the human acetylcholinesterase enzyme (HssAChE).

Methods:
To study the oxidative biotransformation of OP/P=S pesticides and the inhibition of the HssAChE
enzyme by OP/P=S and OP/P=O using the molecular docking technique and QM/MM calculations.

Results:
The theoretical results showed that parathion is the compound with the greatest capacity to transform
its P=S bonds into P=O bonds, thus forming the active paraoxon metabolite in the oxidative biotransformation
process. In the HssAChE inhibition by OP/P=S and OP/P=O, our results showed that of all
the compounds investigated, those with the highest inhibitory activities are parathion, paraoxon, malathion,
diazoxon, chlorpyrifos and omethoate.

Conclusion:
This study was essential due to the lack of information in the literature about the oxidative
biotransformation process of OP/P=S pesticides and the ability of these compounds to inhibit HssAChE.
With this study, it was possible to observe that, in the oxidative biotransformation, chlorpyrifos and parathion
have greater capacities to transform into their active metabolites and in the inhibition of the HssAChE
enzyme, it was possible to observe that not all OF/P=O are the ones with the highest abilities to inhibit
the HssAChE enzyme.
Unhealthy Dietary Pattern Associated with Common Mental Disorders in
Adults and Older Adults: A Population-based Study
Authors: de Melo Simplício Ana Paula , de Almeida Fonseca Viola Poliana Cristina , de Carvalho Lavôr Layanne Cristina , de Lima Sousa Paulo Víctor , de Carvalho Carolina Abreu , Lopes Rodrigues Lays Arnaud Rosal , de Sá  Lustosa Larissa Carvalho Ribeiro , da Silva Pinheiro Michelly , de Lima Jaine Magalhães Paz , de Sousa Ibiapina Cyntia Regina Lucio , de  Oliveira Amanda Ferreira Mesquita , de Moura Crisóstomo Jany , Nunes Lopes Denise Maria , de Azevedo Paiva Adriana , da Silva do Nascimento Vanessa , de Macedo Gonçalves  Frota Karoline 
Published: May 2023

Background::
Mental and behavioral disorders are influenced by biological, psychological
and social factors, especially income, gender, age and family environment, indicating that
physical health is directly associated with mental health. In this perspective, food consumption is
highlighted as a risk factor for mental health, as healthy behaviors, along with a good quality diet,
can reduce symptoms of anxiety and depression.

Objective::
The objective of this a study is to investigate the association between CMD and dietary
patterns, among other lifestyle and socioeconomic factors.

Method::
Cross-sectional population-based and household study conducted with 1574 individuals
aged over 20 years, of both sexes, residing in Teresina and Picos, Piaui, Brazil. Sociodemographic,
lifestyle and food consumption data were collected. The instrument used to identify
the presence of CMDs was the Self Reporting Questionnaire 20 (SRQ-20). Dietary patterns were
obtained using the Principal Component Analysis method.

Results::
Individuals of female sex, separated, with low education, smokers and lower level of
physical activity had a higher prevalence of common mental disorders. As for the eating pattern,
after adjustments, individuals who adhered to the unhealthy eating pattern had a 9% higher prevalence
of CMD. Furthermore, adherence to the white meat standard represented 12% higher prevalence
of CMD.

Conclusion::
The results showed that, regardless of other socioeconomic and lifestyle factors, adherence
to a more monotonous eating pattern in foods, characterized by the mainly consumption
of animal protein and the lack consumption of foods like fruits and vegetables, as well as a less
healthy one, characterized by the consumption of ultra-processed foods, increases the risk of developing
CMD.
Synthesis of S-2-phenylchromane Derivatives and Evaluation of the Antiproliferative
Properties as Apoptosis Inducers in Cancer Cell Lines
Authors: Zhang Yunfeng , Ma Jiale , Pei Yujie , Xie Zeyuan , Fu Dong-Jun , Li Jun 
Published: May 2023

Background:
Cancer remains one of the major health issues globally, where chemotherapy forms the main
treatment mode for different types of cancers. Due to cancer cell ability to develop resistance, decreased clinical effectiveness
of anticancer drugs can occur. Therefore, the need to synthesize novel antitumor drugs remains important.

Objective:
The aim of our work consisted of synthesizing S-2-phenylchromane derivatives containing the tertiary amide
or 1,2,3-triazole fragments with promising anticancer activity.

Methods:
A series of S-2-phenylchromane derivatives were synthesized and evaluated for cytotoxic activity against
three selected cancer cell lines (HGC-27 human gastric carcinoma cell line, Huh-7 epithelial-like tumorigenic cells,
and A549 adenocarcinomic human alveolar basal epithelial cells) using the 3-(4,5-dimethylthiazol-2-yl)-2,5-
diphenyltetrazolium bromide (MTT) assay. Hoechst staining was used to detect the effects of S-2-phenylchromane
derivatives on apoptosis. The apoptosis percentages were detected by annexin V-fluoresceine isothiocyanate/propidium
iodide (Annexin V-FITC/PI) double staining assay with flow cytometry. Expression levels of apoptosis-related proteins
were detected by western blot.

Results:
Cell line A549, consisting of adenocarcinomic human alveolar basal epithelial cells, displayed the highest
sensitivity to the S-2-phenylchromane derivatives. Among these compounds, E2 showed the most potent antiproliferative
activity against A549 cells with an IC50 value of 5.60 μM. Hoechst staining and flow cytometry analysis revealed
apoptosis in A549 cells by compound E2. In addition, activation of the expression levels of caspase-3, caspase-7, and
their substrate poly (ADP-ribose) polymerase (PARP) by E2 was detected by western blot.

Conclusion:
In summary, results point towards compound E2, an S-2-phenylchromane derivative, as a potential lead
molecule in anticancer agents for human adenocarcinomic alveolar basal cells based on the induction of apoptosis.
SARS-CoV-2 Infection and Severity in Patients with Hematologic
Malignancies: A Systematic Review
Authors: SeyedAlinaghi SeyedAhmad , Karimi Amirali , Mirzapour Pegah , Salmani Roghayeh , Razi Armin , Mojdeganlou Hengameh , Mojdeganlou Paniz , Qodrati Mohammad , Jashaninejad Reyhaneh , Paranjkhoo Parinaz , Dadras Omid , Zarezadeh Besharat , Afsahi Amir Masoud , Afzalian Arian , Varshochi Sanaz , Mehraeen Esmaeil , Afsahi Ghazaleh 
Published: May 2023

Introduction:
Earlier reports described the possibility of higher SARS-CoV-2 infection and
severity in patients with hematological malignancies. Given the importance and incidence of these
malignancies, we aimed to systematically review SARS-CoV-2 infection and severity in patients with
hematologic cancers.

Methods:
We retrieved the relevant records by searching the keywords in online databases of PubMed,
Web of Science, Cochrane, and Scopus on December 31st, 2021. A two-step screening; title/abstract
and full-text screening, was employed to select the eligible studies. These eligible studies entered the
final qualitative analysis. The study is adhered to the Preferred Reporting Items for Systematic Reviews
and Meta-Analyses (PRISMA) checklist to ensure the reliability and validity of the results.

Results:
Forty studies concerning different hematologic malignancies and the effect of COVID-19
infection on them were included in the final analysis. The findings showed that in general, the prevalence
of SARS-CoV-2 infection and the severity of the disease are often higher in hematologic malignancies
and the patients could experience higher morbidity and mortality compared to general populations.

Conclusion:
It appeared that individuals with hematologic malignancies are more vulnerable to
COVID-19 infection and they experience more severe disease with higher mortality rates. The presence
of other comorbidities could also deteriorate this situation. Further investigation is recommended
to evaluate the outcome of COVID-19 infection in different subtypes of hematologic malignancies.
Construction of Flower-like ZnO Nanoclusters on Functionalized Graphene
Nanosheets for Room Temperature Formaldehyde Sensing
Authors: Hu Huiyun , Guo Lanpeng , Liang Hongping , Lu Ruofei , Lv Sitao , Wang Chenxu , Liu Liming , Yang Haihong , Lee Yi-Kuen , French Paddy J. , Li Hao , Wang Yao , Zhou Guofu 
Published: May 2023

Background:
Formaldehyde (HCHO) is one of the sources of indoor air pollution
and a recognized carcinogenic gas, which sets a huge threat to human health. Therefore, it is
urgent to develop a formaldehyde gas sensor with high efficiency, low consumption, and low
limit of detection.

Methods:
With solvothermal and supramolecular assembly methods, we fabricate a nanocomposite
of ZnO/5-aminonaphthalene-1-sulfonic acid (ANS)-reduced graphene oxide (rGO)
through in situ assembling flower-like ZnO nanoclusters on ANS-modified graphene nanosheets
for room temperature formaldehyde detection.

Results:
The flower-like ZnO/ANS-rGO based gas sensor exhibits high response (32%, 5 ppm),
ultra-fast response/recovery times (18/23 s), high selectivity, long-term stability and a low practical
limit of detection (pLOD) of 1 ppm toward HCHO at room temperature, offering significant
advantages and competitiveness in chemiresistive room temperature HCHO sensors.

Conclusion:
The unique flower-like nanostructure of ZnO and the functionalization with ANS
molecules jointly improved the HCHO sensing performance of the composite at room temperature.
This work provides a new approach to designing and preparing high-performance room
temperature gas sensing materials.
ग्रामीण भागातील उच्च माध्यमिक विदयालयातील विदयार्थ्यांच्या ऊर्जाबचत अभिवृत्तीचा षोध घेऊन कृतीकार्यक्रमाच्या परिणामकारकतेचा अभ्यास
Authors: पालवे प्रेमकुमार  दिनकर , पोंधे मुकुंद  . 
Published: May 2023

मानवी जीवनात ऊर्जेला अनन्य साधारण महत्व आहे.मानव असो की यंत्र ऊर्जेषिवाय कार्य करणे अषक्य आहे.ऊर्जेला इंग्रजीत एनर्जी म्हणतात .एन म्हणजे आत आणि एरऑन म्हणजे कार्य. अर्थात कार्य करण्याची क्षमता म्हणजे ऊर्जा होय. सूर्य हा ऊर्जेचा मुख्य स्त्रोत आहे. सूर्यापासून पृथ्वीला 174 पेटावॅट ऊर्जा मिळते परंतु ती ऊर्जा रूपांतरीत करण्याच्या अभावामुळे देषावर ऊर्जासंकट ओढावले आहे.पारंपारिक ऊर्जास्त्रोत मर्यादित आहेत, लोकसंख्या प्रचंड प्रमाणात वाढत आहे.यामुळे ऊर्जेची मागणी व पुरवठा यात मोठी तफावत आहे. ग्रामीण भागात फक्त 3 ते 6 तास वीज पुरवठा होतो.भारताचा दरडोई ऊर्जा वापर केवळ 540 किलो वॅट आहे.2025 पर्यंत 34000 मेगावॅटचे अतिरिक्त वीज केंद्र उभारण्याची आवष्यकता आहे. त्यासाठी भविश्यात अपारंपारिक ऊर्जास्त्रोतांचा उपयोग केला तर स्वच्छ आणि मुबलक ऊर्जा मिळेल व ऊर्जेची बचत करता येईल.त्याअनुशंगाने प्रस्तुत संषोधन महत्वाचे आहे. सदर संषोधनासाठी मिश्र पध्दतीचा उपयोग केला आहे.सदर संषोधनात न्यादर्ष म्हणून सर्वेक्षणासाठी ग्रामीण भागातील अहमदनगर जिल्हयातील उच्च माध्यमिक विदयालयातील 300 विदयार्थ्याची निवड केली.श्री आनंद विदयालय चिचोंडी षिराळ या विदयालयातील विदयार्थ्यामध्ये ऊर्जा बचत अभिवृत्ती कमी आढळली. म्हणून या विदयालयातील 27 मुले व 27 मुली अषा एकूण 54 विदयार्थ्याची कृतीकार्यक्रमासाठी सहेतूक निवड केली. माहितीचे संख्याषास्त्रीय विष्लेशण टी परिक्षीकेने करून परिकल्पनेचे सत्यापन पडताळले असता ज परिक्षीका मुल्य 0.3442 आले ते मुल्य 0.01 व 0.05 या स्तरावरील टी मुल्यापेक्षा कमी आहे. म्हणून षुन्य परिकल्पनेचा त्याग करावा लागेल व दोन मध्यमानातील फरक लक्षणीय आहे हे दर्षवणारी परिकल्पना स्विकारावी लागेल.ती म्हणजे ग्रामीण भागातील उच्च माध्यमिक विदयालयातील विदयार्थ्यांच्या ऊर्जाबचत अभिवृत्तीचा षोध घेऊन कृतीकार्यक्रम राबविले असता विदयार्थ्याच्या अभिवृत्तीत लक्षणीय फरक दिसून येतो.	प्रस्तुत संषोधनात ऊर्जा बचतीसाठी अनेक घटकांचा समावेष व सहभाग आहे म्हणून प्रस्तुत संषोधन उपयुक्त आहे.
षिक्षकों की अभिवृत्ति का विद्यार्थियों की षैक्षिक विकास पर प्रभाव का अध्ययन
Authors: गुप्ता प्रतिभा , सिंह उदय 
Published: May 2023

प्राचीन काल से लेकर आज तक षिक्षक को समाज के आदर्ष षिव्यक्तित्व के रूप में स्वीकार किया जाता रहा है षिक्षक ही किसी विद्यालय तथा षिक्षा पद्धति की वास्तविक गत्यात्मक शक्ति है। षिष्य के मन में सीखने की इच्छा को जो जागृत कर पाते है वे ही षिक्षक कहलाते हैं, षिक्षक के द्वारा व्यक्ति के भविष्य को बनाया जाता है एवं षिक्षक ही वह सुधार लाने वाला व्यक्ति होता है विद्यार्थियों को अंधकार से प्रकाष की ओर ले जाता है। षैक्षणिक, क्रियाकलाप की सफलता मुख्य रूप से षिक्षक के अभिवृत्ति, योग्यता एवं उसके कार्य पर निर्भर करती है षिक्षक की अभिवृत्ति जैसी होगा विद्यार्थियों का विकास भी वैसा ही होगा। षिक्षक की सकारात्मक अभिवृत्ति न केवल कार्य को आसान बनाती है, अपितु विद्यार्थियों पर सकारात्मक प्रभाव भी डालती है, जिससे विद्यार्थियों के षैक्षिक उपलब्धि पर सकारात्मक प्रभाव पड़ता है। षिक्षक की नकारात्मक अभिवृत्ति बच्चे में उसी प्रकार की सोच का निर्माण करना सिखाती है। षिक्षकों की विद्यार्थियों के प्रति नकारात्मक अभिवृत्ति से बच्चों में मानसिक तनाव एवं अलगाव की भावना विकसित हो जाती है जो बच्चे के षैक्षिक उपलब्धि पर बुरा प्रभाव डालती है कुछ केसेज में विद्यार्थियों में चिंता की इतनी प्रबल भावना विकसित हो जाती है जो शरीरिक दोष जैसे मांसपेषियों में ऐठन एवं गर्दन मे तनाव उत्पन्न करती है।
कुंजी शब्द: 1. षिक्षको की अभिवृत्ति	2. विद्यार्थियों का षैक्षिक विकास
.