bluuebunny/crossref_metadata_2025_split huggingface dataset:
Protective effects of pentoxifylline against chlorine-induced acute lung injury in rats
Authors: Liu Meng-meng , Liu Jiang-zheng , Zhao Chen-qian , Guo Peng , Wang Zhao , Wu Hao , Yu Weihua , Liu Rui , Hai Chun-xu , Zhang Xiao-di 
Published: February 2023

Abstract
Objective
Chlorine is a chemical threat agent that can be harmful to humans. Inhalation of high levels of chlorine can lead to acute lung injury (ALI). Currently, there is no satisfactory treatment, and effective antidote is urgently needed. Pentoxifylline (PTX), a methylxanthine derivative and nonspecific phosphodiesterase inhibitor, is widely used for the treatment of vascular disorders. The present study was aimed to investigate the inhibitory effects of PTX on chlorine-induced ALI in rats.

Methods
Adult male Sprague-Dawley rats were exposed to 400 ppm Cl2 for 5 min. The histopathological examination was carried out and intracellular reactive oxygen species (ROS) levels were measured by the confocal laser scanning system. Subsequently, to evaluate the effect of PTX, a dose of 100 mg/kg was administered. The activities of superoxide dismutase (SOD) and the contents of malondialdehyde (MDA), glutathione (GSH), oxidized glutathione (GSSG) and lactate dehydrogenase (LDH) were determined by using commercial kits according to the manufacturer’s instructions. Western blot assay was used to detect the protein expressions of SOD1, SOD2, catalase (CAT), hypoxia-inducible factor (HIF)-1α, vascular endothelial growth factor (VEGF), occludin, E-cadherin, bcl-xl, LC 3, Beclin 1, PTEN-induced putative kinase 1 (PINK 1) and Parkin.

Results
The histopathological examination demonstrated that chlorine could destroy the lung structure with hemorrhage, alveolar collapse, and inflammatory infiltration. ROS accumulation was significantly higher in the lungs of rats suffering from inhaling chlorine (P<0.05). PTX markedly reduced concentrations of MAD and GSSG, while increased GSH (P<0.05). The protein expression levels of SOD1 and CAT also decreased (P<0.05). Furthermore, the activity of LDH in rats treated with PTX was significantly decreased compared to those of non-treated group (P<0.05). Additionally, the results also showed that PTX exerted an inhibition effect on protein expressions of HIF-1α, VEGF and occludin, and increased the level of E-cadherin (P<0.05). While the up-regulation of Beclin 1, LC 3II/I, Bcl-xl, and Parkin both in the lung tissues and mitochondria, were found in PTX treated rats (P<0.05). The other protein levels were decreased when treated with PTX (P<0.05).

Conclusion
PTX could ameliorate chlorine-induced lung injury via inhibition effects on oxidative stress, hypoxia and autophagy, thus suggesting that PTX could serve as a potential therapeutic approach for ALI.
Egyptian pediatric clinical practice adapted guidelines: evidence-based [2] steroid-resistant nephrotic syndrome (SRNS) 2022
Authors: Moustafa Bahia , Moselhy Sawsan , Rabie Moftah , Hammad Ayman , Youssef Doaa , Shouman Mohamed , Makar Samuel , Badr Ahmed , Mansour Sameh , Ebrahim Dina , Nabhan Marwa , Ateia Fatma , Abdel-Nabi Hend , Hussein Ahmed , Salman Manal , Korkor Mai S. , Elbahkiry Esraa A. , Dagher Marwa , Selim Abeer , Amer Yasser S. , Omar Tarek , Baky Ashraf Abdel ,   on behalf of the Egyptian Pediatric Clinical Practice Guidelines (EPG) Committee (Chairman Ashraf Abdel Baky), SRNS Clinical & Methodology work group, Badr Ahmed , Attia Fatma , Bahkiry Isra El , Youssef Doaa , Shouman Mohamad , Amer Yasser S. , Sarhan Amr , Soliman Neveen A. , Hakim Ihab El , Zotta Federica 
Published: February 2023

Abstract
Background
Nephrotic syndrome is one of the most common chronic kidney diseases in children. Steroid sensitive type (SSNS) constitutes about 85–90%, whereas steroid-resistant type (SRNS) only 15–20% (Mickinney et al. Pediatr Nephrol 16:1040-1044, 2001). While MCD is the most common histopathology in SS type, children with SRNS have MCD, mesangial proliferative glomerulonephritis, or focal and segmental glomerulosclerosis (FSGS) (International Study Kidney Disease in children, Kidney Int 20;765-771, 1981). SRNS is defined as those who do not show remission after 6 weeks and standard dose of oral steroids ± 3 IV MPD doses (Trautmann et al. Pediatr Nephrol 35:1529-1561, 2020).

Objectives
These national adapted guidelines aim to frame evidence-based recommendations adopted or adapted from the IPNA 2020, KDIGO 2021, and Japanese 2014 de novo guidelines for diagnosis and management of nephrotic children to be presented in two manuscripts: (1) steroid sensitive (SSNS) and (2) steroid-resistant nephrotic syndrome (SRNS).

Methodology
Formulation of key questions was followed with a review of literature guided by our appraised guidelines using AGREE plus appraisal tool. Virtual monthly meetings all through the year 2021 were activated for reviewing and validation of final adaptation evidence-based draft, considering all comments of external reviewers including KDIGO assigned reviewer.

Discussion
Rationale behind the selection of adopted statements and tailoring of others to suit our local facilities, expertise, and our local disease profile was discussed in the text with reasons.

Conclusion
The provided guidelines aim to optimize patient care and outcome and suggest research areas lacking validated research recommendations.
Egyptian evidence -based pediatric clinical practice adapted guidelines for management of [1] steroid sensitive nephrotic syndrome (EPG/SSNS 2022)
Authors: Moustafa Bahia , El-Kersh Mahmoud M. , Shalaby Sherin , Salam Nancy Abdel , Moselhy Sawsan , Soliman Gamal Taha , Selim Abeer , Amer Yasser S , Baky Ashraf Abdel ,   On Behalf of the Egyptian Pediatric Clinical Practice Guidelines (EPG) Committee
Published: February 2023

Abstract
Background
Nephrotic syndrome is one of the most common chronic kidney diseases in children. Steroid sensitive type constitutes about 90% and steroid resistant 10% of total cases.

Objectives
These national adapted guidelines aim to frame evidence-based recommendations adopted or adapted from IPNA 2020, KDIGO 2021, and Japanese 2014 for diagnosis, evaluation, management and follow-up of nephrotic children for Steroid sensitive nephrotic syndrome (SSNS) as paper one to be followed with SRNS as paper two.

Methodology
Formulation of key questions was followed with review of literature, guided by our retrieved and appraised guidelines using Agree plus appraisal tool. After virtual monthly meetings through the year 2021, the final draft was validated considering the comments of external local reviewers and KDIGO-assigned reviewers.

Discussion
Rationale behind the selection of adopted statements and tailoring of others to suit our local facilities’ expertise and disease profile was discussed in the text with reasons.

Conclusion
The provided guidelines aim to optimize patient care and outcome and suggest research areas lacking validated research recommendations.
Imaging distributed acoustic sensing-to-geophone conversion data: A field application to CO2 sequestration data
Authors: Ma Yong , Fu Lei , Li Weichang 
Published: February 2023

Compared with conventional geophone data, distributed fiber-optic sensing, including distributed acoustic sensing (DAS), can provide better spatial coverage for imaging the subsurface with finer spatial sampling. Because DAS measures subsurface seismic responses differently than the geophone, imaging technologies (e.g., reverse time migration and full-waveform inversion) that are developed for conventional geophone data cannot be readily applied to original DAS data without causing uncertainties in phase or depth, especially when one compares the DAS imaging results against the usual geophone imaging results. Based on vertical seismic profile field data from a CO2 sequestration site, we have compared the imaging results of the CO2 storage reservoir associated with the DAS and the geophone data, respectively, and we illustrate the differences between the imaging results of the DAS and geophone data. The difference between the DAS and geophone imaging results could be critical in obtaining time-lapse signals for monitoring reservoir changes, e.g., in subsurface CO2 sequestration. We propose to convert DAS to geophone data so that we can reduce the discrepancies between DAS and geophone imaging results and we therefore can reuse existing seismic imaging technologies. Two conversion methods, one physics-based and one deep-learning (DL)-based, are used for the DAS-to-geophone transformation. Field data results demonstrate that the DL-based approach can better successfully improve the alignment between the DAS and geophone images, whereas the physics-based solution is constrained by its assumption.
Optimized Detection of Unknown
MET
Exon 14 Skipping Mutations in Routine Testing for Patients With Non–Small-Cell Lung Cancer
Authors: Sun Rui , Wang Zhizhong , Zhao Jiuzhou , Ren Pengfei , Ma Jie , Guo Yongjun 
Published: February 2023

PURPOSE

MET
exon 14 (
MET
ex14) skipping is an actionable biomarker in non–small-cell lung cancer. However,
MET
variants are highly complex and diverse, and not all variants lead to exon 14 skipping. Assessing the skipping effect of unknown variants is still a key issue in molecular diagnosis.

MATERIALS AND METHODS

We retrospectively collected
MET
variants around exon 14 from 4,233 patients with non–small-cell lung cancer who underwent next-generation sequencing testing using DNA, as well as two published data sets.

RESULTS
Among the 4,233 patients, 44 unique variants including 29 novel variants (65.9%) were discovered from 53 patients. Notably, 31 samples (58.5%) failed RNA verification. Using RNA verification, nine novel skipping variants and five nonskipping variants were confirmed. We further used SpliceAI with the delta score cutoff of 0.315 to aid the classification of novel variants (sensitivity = 98.88% and specificity = 100%). When applied to the reported variants, we also found three wrongly classified nonskipping variants. Finally, an optimized knowledge-based interpretation procedure for clinical routine was built according to the mutation type and location, and five more skipping mutations from the 13 unknown variants were determined, which improved the population determination rate to 0.92%.

CONCLUSION

This study discovered more
MET
ex14 skipping variants and optimized an innovative approach that could be adapted for the interpretation of infrequent or novel
MET
ex14 variants timely without experimental validation.
A Case of Lymphocytic Infundibulo-neurohypophysitis Exhibiting Spontaneous Regression
Authors: Hasebe Masashi , Shibue Kimitaka , Honjo Sachiko , Hamasaki Akihiro 
Published: February 2023

Abstract
Lymphocytic infundibulo-neurohypophysitis (LINH) is a rare autoimmune inflammatory process that selectively affects the neurohypophysis and the pituitary stalk, typically presenting with central diabetes insipidus (CDI). LINH is considered underdiagnosed because the definitive diagnosis requires invasive pituitary surgery with a high risk of complications. We present a case of CDI resulting from LINH, which was treated with conservative management, eschewing both glucocorticoid treatment and pituitary surgery. At presentation, the hormonal assessment indicated the presence of CDI without anterior pituitary dysfunction. Magnetic resonance imaging revealed stalk thickening without a posterior pituitary bright spot, and anti-rabphilin-3A antibodies were positive in serum. Collectively, we made a diagnosis of LINH. Considering that the patient did not exhibit any symptoms of mass effect, we chose conservative treatment with desmopressin acetate. One year later, the stalk thickening regressed spontaneously without surgical or glucocorticoid treatment, although the posterior pituitary bright spot remained absent, and CDI did not improve. The inflammatory process of LINH is mostly self-limited and recovers spontaneously, whereas life-long desmopressin treatment may be required because of pituitary stalk fibrosis and atrophy. Our case highlights the importance of noninvasive diagnosis and careful follow-up in preventing unnecessary interventions for patients with LINH.
Serum Adiponectin and Leptin Is Not Related to Skeletal Muscle Morphology and Function in Young Women
Authors: Yoshiko Akito , Ohta Megumi , Kuramochi Rieko , Mitsuyama Hirohito 
Published: February 2023

Abstract
Adipokines secreted from adipose tissue, such as adiponectin and leptin, enhance skeletal muscle metabolism. Animal studies have shown that adipokine knockout leads to a reduction in muscle function. Muscle function is determined by muscle size and quality; therefore, it is speculated that lower adipokine levels affect skeletal muscle size and quality, eventually leading to lower muscle function. This study aimed to investigate the relationship between adipokines and skeletal muscle morphology and function in young individuals. A total of 21 young women participated in this study. Adiponectin and leptin levels were analyzed using fasting blood samples from all participants. B-mode ultrasound images of the thigh and calf were obtained, and the muscle thickness and echo intensity were measured in the vastus lateralis (VL) and medial gastrocnemius (MG). The shear modulus was measured from the VL and MG using shear wave elastography. Knee extension and plantar flexion peak torques were measured as muscle functions. Adiponectin and leptin were not related to echo intensity, shear modulus, and muscle thickness in the VL and MG (rs = −0.26−0.37, P &gt; .05). Furthermore, no relationship was observed between adiponectin, leptin, knee extension, and dorsiflexion peak torque (rs = −0.28−0.41, P &gt; .05). These negative results suggest that adiponectin and leptin levels in young women are not associated with muscle size and quality, nor are they related to muscle function.
Efficacy of Moringa oleifera Lam. extracts and Pediococcus pentosaceus, Lactobacillus acidophilus, Lactobacillus plantarum probiotic during starter period on growth performance of male broiler chicken
Authors: Karwanti Novita Wanda , Arumdani Dynda Febriana , Yulianto Andreas Berny , Marbun Tabita Dameria , Sherasiya Anjum , Al Arif Mohammad Anam , Lamid Mirni , Lokapirnasari Widya Paramita 
Published: February 2023

Background: Probiotics and medicinal plants have been used to support human and livestock health. This research aimed to evaluate the potential of Moringa oleifera Lam. leaf extract on the growth of Pediococcus pentosaceus, Lactobacillus acidophilus and L. plantarum probiotic in vitro and to prove its potential as a feed additive, as an alternative to antibiotic growth promoters (AGP) to improve nutrient intake, body weight gain, feed conversion ratio and feed efficiency in broiler chicken starter phase. Methods: This study consisted of three sub studies: (1) Screening test for phytochemical compounds; (2) evaluation of several doses of Moringa extract (0%, 0.1%, 0.2%, 0.3% ). At the end of treatment, each colony was measured using the total plate count; (3) evaluation of probiotics and M. oleifera in vivo to prove growth performance on starter-phase broiler chicken. All results were analyzed by analysis of variance (ANOVA) then followed by the Duncan test. Results: (1) The phytochemical screening test M. oleifera extract contained flavonoids, saponins, tannins, triterpenoids and alkaloids; (2) M. oleifera extract at doses of 0.1%, 0.2%, 0.3% increased the growth of bacteria Pediococcus pentosaceus, Lactobacillus acidophilus and Lactiplantibacillus plantarum (p<0.05); (3) The use of probiotics, M. oleifera extract and their combination showed that body weight, body weight gain, feed conversion rate (FCR) and feed efficiency signifcantly differed (p <0.05) between the treatments, but there was no significant difference (p > 0.05) between the treatments on nutrient intake (dry matter, ash, crude protein, crude fiber and organic matter) on broiler chicken starter phase. Conclusions: The use of M.oleifera extract at doses of 0.1%, 0.2% and 0.3% increased the growth of P. pentosaceus, L. acidophilus and L. plantarum bacteria in vitro and the use of probiotics, M.oleifera extract and their combination by in vivo improved the growth performance on starter phase of broilers chicken.
A qualitative study exploring hand hygiene practices in a neonatal unit in Blantyre, Malawi: implications for controlling healthcare-associated infections
Authors: Mangochi Helen , Tolhurst Rachel , Simpson Victoria , Kawaza Kondwani , Chidziwisano Kondwani , Feasey Nicholas A. , Morse Tracy , MacPherson Eleanor 
Published: February 2023

Background: Neonatal sepsis is responsible for a considerable burden of morbidity and mortality in sub-Saharan African countries. Outcomes from neonatal sepsis are worsening due to increasing rates of antimicrobial resistance. Sub-optimal Infection Prevention and Control (IPC) practices of health care workers and caregivers are important drivers of infection transmission. The Chatinkha Neonatal Unit at Queen Elizabeth Central Hospital, Blantyre, Malawi has experienced multiple outbreaks of neonatal sepsis, associated with drug resistant Klebsiella pneumoniae. We aimed to understand the barriers to implementation of optimal IPC focusing on hand hygiene practice. Methods: We used a qualitative research methodology to meet the study aim. Combining participant observation (PO) over a seven-month period with semi structured interviews (SSI) to provide an in-depth understanding of activities relating to hygiene and IPC existing on the ward. Results: While most staff and some caregivers, had a good understanding of ideal IPC and understood the importance of good handwashing practices, they faced substantial structural limitations, and scarce resources (both material and human) which made implementation challenging. For staff, the overwhelming numbers of patients meant the workload was often unmanageable and practicing optimal IPC was challenging. Caregivers lacked access to basic amenities, including linen and chairs, meaning that it was almost impossible for them to maintain good hand hygiene. Limited access to soap and the erratic water supply for both caregivers and healthcare workers further worsened the situation. Communication challenges between different cadres of staff and with patient caregivers meant that those handling neonates and cleaning the wards were often unaware of outbreaks of drug resistant infection. Conclusion: For IPC to be improved, interventions need to address the chronic shortages of material resources and create an enabling environment for HCWs and patient caregivers.
The Gendering of Job Postings in the Online Recruitment Process
Authors: Castilla Emilio J. , Rho Hye Jin 
Published: February 2023

Gender segregation remains a significant problem in many occupations and organizations. To solve this problem, many U.S. employers now seek to craft gender-neutral job postings. In this article, we investigate whether such employer recruitment efforts are successful in encouraging women and men to apply equally for jobs. Specifically, we move beyond the well-studied effects of the gender typing of occupations, organizations, and industries to study the extent to which the recruiting language used in job postings influences the actual preapplication behavior of job seekers of different genders. Using unique data from both a large-sample observational field study (Study 1) and a field experiment study (Study 2) of real online job postings, we first assess the gendered language mechanism by testing whether stereotypical femininity in the wording that recruiters use to advertise otherwise identical jobs differently influences female and male job seekers’ interest in applying. We then assess the recruiter gendering mechanism by testing whether the gender of the recruiter and the femininity in the wording recruiters use when presenting themselves to job seekers further contribute to gender job search disparities. Our analyses ultimately show negligible effects for both the gendering of job postings or of the job poster, and we therefore conclude that, in practice, employers’ efforts to simply tweak the language of recruitment messages do not matter much for gender equality and diversity. This paper was accepted by Olav Sorenson, organizations. Funding: The authors received financial support from the James S. Hardigg (1945) Work and Employment Fund and the Massachusetts Institute of Technology Sloan School of Management. Supplemental Material: The data files are available at https://doi.org/10.1287/mnsc.2023.4674 .
infância e experiência em walter benjamin
Authors: Santana Eduarda Aleycha luciano , De oliveira Paula Ramos 
Published: February 2023

Walter Benjamin is a seminal philosopher whose work makes us think about the concepts of experience, child, and childhood. A journey through his life experience and personal narratives brings us closer to the world of his own childhood. As such, it is a philosophical journey in the sense of taking us towards something that is unknown to us and that in the end can transform us. We will travel through the following texts by Benjamin: Children’s Hour Radio Narrative (2015), Berlin Childhood: around 1900 (2013), Reflections on the child, the toy and education (2009), One-Way Street (2013) and The Storyteller:Reflections on the works of Nikolai Leskov (2018). To navigate these texts is to allow and encourage us to experiment with other ways of looking at and being in the world. His narrative allows us to experience the experiences of the child Benjamin, to traverse its unique and sparsely inhabited places, and thus to travel through any child’s world, feeling a child’s temporality and a child’s gaze. According to Jorge Larrosa (2003), childhood is an enigma. Benjamin’s narratives allow us to enter this enigma, to unveil images redolent with the experience of children and childhood, and thereby to approach this mystery that is both philosophical and pulsating with life, like the life of this philosopher himself.
ao rosto do adulto, o gesto de alice nas cidades
Authors: Costa Victor Anselmo , Kasper Kátia Maria 
Published: February 2023

One scene from Wim Wenders’ film “Alice in the cities” provokes us to think again about childhood. It’s a fresh polaroid snapshot, where we can see the faces of an adult (Phillip Winter) and a child (Alice) blending and mixing. Starting from this image, this paper makes its way through the movie’s plot, reflecting on the power of freedom that arises from an encounter with children and childhood. First, we examine the relationship between Winter and his job as a photo-journalist, discussing the character of his existential anguish and his supposed indisposition to becoming, which seems to be clear in the way that he reads his own photographs. In a second moment of the film, we understand Alice’s arrival on the scene as something capable of dispersing the circumstances that are constraining him by hurling him into the unpredictable. This event is crystallized in one poetic gesture: the girl confronts the adult with her own face by drawing a self-portrait. Facialization and deterritorialization are twoconcepts that we weave into our discussion, working off the philosophical thinking of Georges Didi-Huberman, Gilles Deleuze and Félix Guattari. Finally, we propose that worry as a value, characteristic of children and travelers, can produce an opening that offers an escape into a freedom of release–or, so to speak, a defacialized freedom.