bluuebunny/crossref_metadata_2025_split huggingface dataset:
Structural basis for the ligand promiscuity of the neofunctionalized, carotenoid-binding fasciclin domain protein AstaP
Authors: Kornilov Fedor D. , Slonimskiy Yury B. , Lunegova Daria A. , Egorkin Nikita A. , Savitskaya Anna G. , Kleymenov Sergey Yu. , Maksimov Eugene G. , Goncharuk Sergey A. , Mineev Konstantin S. , Sluchanko Nikolai N. 
Published: April 2023

AbstractFasciclins (FAS1) are ancient adhesion protein domains with no common small ligand binding reported. A unique microalgal FAS1-containing astaxanthin (AXT)-binding protein (AstaP) binds a broad repertoire of carotenoids by a largely unknown mechanism. Here, we explain the ligand promiscuity of AstaP-orange1 (AstaPo1) by determining its NMR structure in complex with AXT and validating this structure by SAXS, calorimetry, optical spectroscopy and mutagenesis. α1-α2 helices of the AstaPo1 FAS1 domain embrace the carotenoid polyene like a jaw, forming a hydrophobic tunnel, too short to cap the AXT β-ionone rings and dictate specificity. AXT-contacting AstaPo1 residues exhibit different conservation in AstaPs with the tentative carotenoid-binding function and in FAS1 proteins generally, which supports the idea of AstaP neofunctionalization within green algae. Intriguingly, a cyanobacterial homolog with a similar domain structure cannot bind carotenoids under identical conditions. These structure-activity relationships provide the first step towards the sequence-based prediction of the carotenoid-binding FAS1 members.
The Newfoundland and Labrador mosaic founder population descends from an Irish and British diaspora from 300 years ago
Authors: Gilbert Edmund , Zurel Heather , MacMillan Margaret E. , Demiriz Sedat , Mirhendi Sadra , Merrigan Michael , O’Reilly Seamus , Molloy Anne M. , Brody Lawrence C. , Bodmer Walter , Leach Richard A. , Scott Roderick E. M. , Mugford Gerald , Randhawa Ranjit , Stephens J. Claiborne , Symington Alison L. , Cavalleri Gianpiero L. , Phillips Michael S. 
Published: April 2023

AbstractThe founder population of Newfoundland and Labrador (NL) is a unique genetic resource, in part due to its geographic and cultural isolation, where historical records describe a migration of European settlers, primarily from Ireland and England, to NL in the 18th and 19th centuries. Whilst its historical isolation, and increased prevalence of certain monogenic disorders are well appreciated, details of the fine-scale genetic structure and ancestry of the population are lacking. Understanding the genetic origins and background of functional, disease causing, genetic variants would aid genetic mapping efforts in the Province. Here, we leverage dense genome-wide SNP data on 1,807 NL individuals to reveal fine-scale genetic structure in NL that is clustered around coastal communities and correlated with Christian denomination. We show that the majority of NL European ancestry can be traced back to the south-east and south-west of Ireland and England, respectively. We date a substantial population size bottleneck approximately 10-15 generations ago in NL, associated with increased haplotype sharing and autozygosity. Our results reveal insights into the population history of NL and demonstrate evidence of a population conducive to further genetic studies and biomarker discovery.
Bidirectional wave-propelled capillary spinners
Authors: Barotta Jack-William , Thomson Stuart J. , Alventosa Luke F. L. , Lewis Maya , Harris Daniel M. 
Published: April 2023

AbstractWhen a solid body floats at the interface of a vibrating liquid bath, the motion of the object generates outwardly propagating surface waves. We here demonstrate that chiral objects on a vibrating fluid interface are set into steady rotation, with the angular speed and direction of rotation controlled by the interplay between object geometry and driving parameters. Scaling laws and a simplified model of the wavefield reveal the underlying physical mechanism of rotation, while collapsing measurements of the angular velocity across parameters. Leveraging the control over the chiral object’s direction of rotation, we demonstrate that a body with an asymmetric mass distribution and chirality can be remotely steered along two-dimensional trajectories via modulation of the driving frequency. This accessible and tunable macroscopic system serves as a potential platform for explorations of chiral active and driven matter, and demonstrates a mechanism by which wave-mediated forces can be manipulated for directed propulsion.
Prefectures vulnerable to water scarcity are not evenly distributed across China
Authors: Zhao Fubo , Wang Xi , Wu Yiping , Singh Shailesh Kumar 
Published: April 2023

AbstractWater scarcity has become a significant constraint to sustainable development. While the water scarcity has been widely assessed, its social impacts are infrequently evaluated. Here, we developed a framework to integrate the water scarcity and climate sensitivity to examine social vulnerability at the prefecture level across China. Results showed that 41% of the 301 prefectures were highly water stressed and 30% were highly sensitive to changing climate. We identified 44 hotspot prefectures using a combination of high water scarcity and climate sensitivity. We mapped the gradient in social vulnerability to water stress and climate sensitivity in these 44 prefectures and found that prefectures with ‘very high’ social vulnerability were primarily distributed in Central and Southwest China. These ‘very high’ vulnerable prefectures are home to more than 58 million people. The consideration of both water scarcity and climate sensitivity across management units has potential to formulate policies regarding water resources management.
Interpreting the cosmic ray spectrum and composition measurements across the ankle and up to the highest energies with the data of the Pierre Auger Observatory
Authors: Guido Eleonora 
Published: April 2023

In this work we present a combined fit of the energy spectrum and mass composition data above 6×1017 eV as measured by the Pierre Auger Collaboration and we show that our data can be reproduced by the superposition of two (or more) components, either Galactic or extragalactic. We use a simple astrophysical model with two extragalactic components, one dominant at high energy and the other at low energy, whose superposition generates the so-called “ankle” feature. We find that the component dominating the low (high) energy region is required to be emitted from the sources with a very soft (hard) spectrum. In our best fit scenario the sources contributing above the ankle emit a mixed and increasingly heavier mass composition, whereas a mix of protons and intermediate-mass nuclei is observed below the ankle, which reach the Earth almost una↵ected by the propagation in the intergalactic space. The possible presence of a Galactic population providing the intermediate-mass contribution at low energy is also explored.
In order to evaluate our capability to constrain astrophysical models, we finally discuss the impact on the fit results of the main experimental systematic uncertainties and of the assumptions on the quantities a↵ecting propagation, both in the atmosphere and in the intergalactic medium.
Study on COVD-19 epidemic prediction and distribution Strategy based on SIR Model
Authors: Liu Mengqi , Wang Pengyu , Zhao Zhifeng 
Published: April 2023

Developing a more scientific COVID-19 response strategy has become a hot topic today. By sorting out a large amount of data, this paper predicts the spread law of the epidemic in Shanghai, Beijing, and Changchun in May, summarizing the key factors related to the spread of the epidemic and human immunity, using a tool to process and fitting the data, obtaining the spread law of the COVID-19 outbreak in the three cities under the epidemic, and establishing the SIR Infectious disease model. The development of the epidemic situation in the above towns was analyzed and predicted by linear fitting.
In than in Shanghai, Beijing, and Changchun, three cities the size of the population, social and economic situation, policy enforcement, and other factors, we select the optimal strategy of Shanghai medical collections as the research object to analyze the degree of Shanghai regional deficiencies, and supplies reasonable dispatch by using principal component analysis and cluster analysis method.
Identification of ADA as a Biomarker for Atypical Epstein Barr Virus
Infection in Children
Authors: Shi Ting , Huang Linlin , Zhang Wangsheng , Shi Wenxin , Wu Jiahui , Bian Yuanxi , Jiang Yanqun , Tian Jianmei 
Published: April 2023

Abstract
Objective This study aims to explore the ability of adenosine deaminase
(ADA) to discriminate atypical Epstein Barr virus (EBV) infection in children
from acute febrile illness.
Methods All children admitted to the Children’s Hospital of
Soochow University between 2018 and 2019, who were acute febrile patients and
subjected to the plasma EBV-DNA polymerase chain reaction (PCR) and indirect
immunofluorescence (IIF) assay for EBV-specific antibodies assays. The
diagnostic value of each detection index was compared by the area under the ROC
curve.
Results In children with atypical Epstein Barr virus infection, the
sensitivity, specificity, positive predictive value, negative predictive value
and Youden index were 62.87%, 100.00%,100.00%,
61.73% and 0.63 for EBV-DNA PCR assay, 80.84%, 100.00%,
100.00%, 75.76% and 0.81 for VCA-IgG avidity and 89.22%,
87.00%, 91.98%, 82.86% and 0.76 for ADA. VCA-IgG avidity
(AUC=0.904, P<0.01) and ADA (AUC=0.881, P<0.01)
assays had the great diagnostic efficiency. In addition, the sensitivity,
specificity and AUC were 92.75%,91.43% and 0.921(95%CI:
0.856–0.985) for ADA in the course≤3 days group,
respectively.
Conclusions ADA has a good diagnostic value in the early stage of atypical
EBV infection, and is not affected by primary EBV infection and
reactivation.
Schlüsselwörter Adenosine deaminase, Epstein -Barr virus,
Biomarker, children.
Silyl Esters as Reactive Intermediates in Organic Synthesis
Authors: Adler Marc J. , D’Amaral Melissa C. , Andrews Keith G. , Denton Ross 
Published: April 2023

AbstractSilyl esters have been exploited as metastable reaction intermediates, both purposefully and unintentionally, since at least the 1960s. Their reactivity is broadly related to the substituents on the silicon, and in this way their properties can be readily modulated. Silyl esters have unique reactivity profiles that have been used to generate downstream products of a range of functionalities, and because of this many excellent methods for the synthesis of a variety of value-added chemicals have been developed. Furthermore, because of the frequent use of hydrosilanes as terminal reductants in catalytic processes, silyl ester intermediates are likely more commonly utilized by synthetic chemists than currently realized. This review comprehensively summarizes the reactions known to take advantage of reactive silyl ester intermediates and discusses examples of catalytic reactions that proceed in an unanticipated manner through silyl ester intermediates.1	Introduction2	Synthesis of Silyl Esters3	Making Amides from Silyl Esters3.1	Amidation Using Chlorosilanes3.2	Amidation Using Azasilanes3.3	Amidation Using Oxysilanes3.4	Amidation Using Hydrosilanes3.5	Amine Formation via Amidation/Reduction3.6	Miscellaneous4	Mechanistic Investigations of Amidation4.1	Mechanism of Amidation Using Chlorosilanes4.2	Mechanism of Amidation Using Hydrosilanes4.3	Mechanism of Amidation Using Oxy- or Azasilanes5	Making Esters from Silyl Esters6	Making Aldehydes, Alcohols, Amines, and Alkanes via Reduction6.1	Aldehyde Synthesis by Metal-Free Reduction6.2	Aldehyde Synthesis by Metal-Mediated Reduction6.3	Alcohol Synthesis by Metal-Mediated Reduction6.4	Amine Synthesis6.5	Alkane Synthesis by Metal-Free Reduction7	Making Acid Chlorides from Silyl Esters8	In Situ Generated Silyl Esters and Ramifications for Catalysis9	Conclusion
Induction of Migration and Collagen Synthesis in Human Gingival Fibroblasts Using Periodontal Ligament Stem Cell Conditioned Medium
Authors: Banlue Akkapol , Kaewmuangmoon Jirattikarn , Janebodin Kajohnkiart , Tansriratanawong Kallapat 
Published: April 2023

Abstract
Objective This study aimed to examine the effect of periodontal ligament stem cell conditioned medium (PDLSC-CM) on human gingival fibroblast (HGF) migration and collagen synthesis.
Materials and Methods To assess cell viability, we extracted PDLSC-CM, and the total derived protein concentration was adjusted to 12.5 to 200 µg/mL, followed by treatment with HGFs. The viability of HGFs was observed for 24 hours using the MTT assay. Cell migration was monitored for 24 to 48 hours by wound healing and Boyden chamber assays. Collagen synthesis from HGFs was examined by picrosirius red dye and real-time polymerase chain reaction (PCR) to measure collagen type I and III gene expression for 7 to 10 days. A comparison among the groups was assessed using a one-way analysis of variance (ANOVA) and Bonferroni post hoc test, with the exception of the cell viability assay, which was subjected to Welch's test and Dunnett's T3 post hoc test.
Results HGF viability was significantly enhanced by 12.5, 25, and 50 µg/mL PDLSC-CM. The HGFs treated with 50 µg/mL PDLSC-CM promoted cell migration as shown by wound healing and Boyden chamber assays. At this concentration, collagen synthesis increased at 10 days. Collagen type I gene expression increased by 1.6-fold (p < 0.001) and 4.96-fold (p < 0.001) at 7 and 10 days, respectively. Collagen type III gene expression showed an increase of 1.76-fold (p < 0.001) and 6.67-fold (p < 0.001) at the same time points.
Conclusion Our study suggested that a low concentration of PDLSC-CM at 50 µg/mL has given an amelioration of HGFs providing for periodontal wound healing and periodontal regeneration, particularly migration and collagen synthesis.
Standardization of Latency and Amplitude Values of Short, Middle and Long Latency Auditory Evoked Potentials in Adults
Authors: Pelaquim Andressa , Sanfins Milaine Dominici , Fornazieri Marco Aurélio 
Published: April 2023

Abstract
Introduction Auditory processing refers to the efficiency and effectiveness with which the central auditory nervous system uses auditory information. Middle- and long-latency auditory evoked potentials are objective electrophysiological tests that can complement the diagnosis of alterations involving central auditory processing.
Objective To standardize latency and amplitude values for short-, middle-, and long-latency auditory evoked potentials in adults with normal hearing thresholds.
Methods This is a cross-sectional study. Thirty-three adults with normal hearing thresholds, without hearing complaints, and with normal central auditory processing were evaluated. All underwent basic audiological evaluation, central auditory processing assessment, and short-, middle-, and long-latency auditory evoked potentials.
Results Absolute latency and interpeak values for middle- and long-latency auditory evoked potentials were lower than internationally suggested. However, for the brainstem auditory evoked potential, the means were within the range considered as normal, as suggested in the equipment.
Conclusion The present study provided measurements of normal latencies and amplitudes for short-, middle-, and long-latency auditory evoked potentials in adults.
Etiology and Epidemiology of Nasal Bone Fractures in Patients Referred to the Otorhinolaryngology Section, 2019
Authors: Davari Rahim , Pirzadeh Akbar , Sattari Fatemeh 
Published: April 2023

Abstract
Introduction One of the most observed diseases in the otorhinolaryngology emergency, compared with the other facial fractures, is related to nasal bone fractures (NBFs). The peak of incidence is seen in the age group ranging from 11 to 30 years old.
Objective The present evaluation was devoted to the etiology and epidemiological study of NBFs.
Methods In the present cross-sectional study, 376 patients with NBF were evaluated. The necessary information such as gender, age, education, job, causes of NBF, and clinical symptoms of patients have been recorded on the checklist.
Results The study revealed that 76.9% of the patients were male and 23.1% were female; 37.5% of all patients were self-employed, and most of them were from urban areas. Traffic accident (26.6%) and falling (25.5%) were the main reasons for NBF. The most common clinical symptoms for NBF were tenderness (96%; n = 361), nasal swelling (90.4%; n = 340), and deformity (89.4%; n = 336).
Conclusion The results showed that the incidence of NBFs in young men without higher education level and self-employed were high which can be related to the traffic accidents and fights. Also, falls, beatings and accidental hit are the most common causes of NBFs among women. Therefore, to decrease the incidence of otorhinolaryngology trauma, training about the general life skill and providing awareness about using personal safety equipment and measures should be increased at the future.
Technologies Applied to the Mental Health Care of Pregnant Women: A Systematic Literature Review
Authors: Carvalho Laís Lage de , Teixeira Júlia Magna da Silva , Unger Roberto José Gervásio , Motti Vivian Genaro , Lovisi Giovanni Marcos , Grincenkov Fabiane Rossi dos Santos 
Published: April 2023

Abstract
Objective: This article aims to review the literature regarding the use of technologies to promote mental health for pregnant women. We seek to: understand the strategies that pregnant women use for mental health care. Also, we investigate the existence of scientific evidence that validates such practices.
Methods: This study follows the PRISMA guidelines for systematic reviews. We analyze 27 studies published between 2012 and 2019. We include publications in Portuguese, English, and Spanish.
Results: The results revealed several different possibilities to use technology, including the use of text messages and mobile applications on smartphones. Mobile applications are the most commonly used approaches (22.5%). Regarding the strategies used, cognitive-behavioral approaches, including mood checks, relaxation exercises, and psychoeducation comprised 44.12% of the content.
Conclusion: There is a need for further investigation and research and development efforts in this field to better understand the possibilities of intervention in mental health in the digital age.
Advance Care Planning and Parent-Reported End-of-Life Outcomes in the Neonatal Intensive Care Unit
Authors: Lin Matthew , Williams David , Vitcov Giselle , Sayeed Sadath , DeCourcey Danielle D. , Wolfe Joanne , Cummings Christy 
Published: April 2023

Objective This study aimed to evaluate the impact of advance care planning (ACP) on parent-reported end-of-life (EOL) outcomes in the neonatal intensive care unit (NICU).
Study Design Single-center, cross-sectional mixed-methods survey study of bereaved parents who experienced the death of a child in the Boston Children's Hospital NICU between 2010 and 2021. Logistic regression, chi-square test, Fisher's exact test, and Wilcoxon rank-sum test were used to evaluate the relationship between ACP and parent-reported EOL outcomes. Qualitative content was analyzed through inductive coding.
Results A total of 40/146 (27%) of eligible parents responded to our survey. There was a significant association between ACP and improved EOL care processes and parental satisfaction with communication. Parents with ACP were more likely to report goal-concordant care and higher levels of perceived shared decision-making. Qualitatively, emerging themes in parents' descriptions of goal-concordant care included misaligned expectations and communication. Emerging themes for parental preparedness included infant symptoms, logistical aspects, impact on parents, and degrees of preparedness. For decisional regret, the primary theme was reevaluating supports.
Conclusion ACP is associated with improved EOL care outcomes and parents qualitatively conceptualize goal-concordant care, preparedness for their child's death, and decisional regret in nuanced ways. Families should have the opportunity to participate in ACP discussions that meet their unique communication and decision-making needs.
Key Points
Integrating Oldenburg’s concept of place and Soja’s concept of space: a spatial enquiry of denial, repression, and closure in Ishiguro’s “The Remains of the Day”
Authors: Maran Aishwarya , J Michael Raj 
Published: April 2023

AbstractSpatial enquiry in literature has opened new doors for understanding the relationship between physical and mental space. This paper analyses how an individual’s sense of space can influence their understanding of themselves and others. This study identifies the similarities between Ray Oldenburg’s concept of place and Edward Soja’s concept of space. Moreover, it integrates both theories by locating Oldenburg’s triad of place (home, workplace, and third place) as a part of Soja’s concept of real space (firstspace). The lack of discernibility between home and workspace exerts itself in protagonist Stevens’ obsession with his profession. This paper also examines how disorientation in the trifold conception of place causes Stevens to be an emotionally and spatially repressed character. The study elaborates on the role of third place and thirdspace. It also analyses Steven’s six-day journey into the countryside as an introspective journey into his past. This study aims to find how social interactions in the third place have helped Stevens unlearn and relearn hegemonically influenced ideologies on duty and dignity. Further, it helps Stevens resolve his denial and repression, properly channelling the flow of spatial energy. This study also identifies thirdspace as a transformative space that helps Stevens find closure and accept his regretful past.
A sub-2 Kelvin cryogenic magneto-terahertz scattering-type scanning near-field optical microscope (cm-THz-sSNOM)
Authors: Kim R. H. J. , Park J.-M. , Haeuser S. J. , Luo L. , Wang J. 
Published: April 2023

We have developed a versatile near-field microscopy platform that can operate at high magnetic fields and below liquid-helium temperatures. We use this platform to demonstrate an extreme terahertz (THz) nanoscope operation and to obtain the first cryogenic magneto-THz time-domain nano-spectroscopy/imaging at temperatures as low as 1.8 K, magnetic fields of up to 5 T, and with operation of 0–2 THz. Our Cryogenic Magneto-Terahertz Scattering-type Scanning Near-field Optical Microscope (or cm-THz-sSNOM) instrument is comprised of three main equipment: (i) a 5 T split pair magnetic cryostat with a custom made insert, (ii) a custom sSNOM instrument capable of accepting ultrafast THz excitation, and (iii) a MHz repetition rate, femtosecond laser amplifier for broadband THz pulse generation and sensitive detection. We apply the cm-THz-sSNOM to obtain proof of principle measurements of superconductors and topological semimetals. The new capabilities demonstrated break grounds for studying quantum materials that require an extreme environment of cryogenic operation and/or applied magnetic fields in nanometer space, femtosecond time, and THz energy scales.