bluuebunny/crossref_metadata_2025_split huggingface dataset:
Targeting the Clear Cell Sarcoma Oncogenic Driver Fusion Gene EWSR1::ATF1 by HDAC Inhibition
Authors: Mae Hirokazu , Outani Hidetatsu , Imura Yoshinori , Chijimatsu Ryota , Inoue Akitomo , Kotani Yuki , Yasuda Naohiro , Nakai Sho , Nakai Takaaki , Takenaka Satoshi , Okada Seiji 
Published: June 2023

Clear cell sarcoma (CCS), a rare but extremely aggressive malignancy with no effective therapy, is characterized by the expression of the oncogenic driver fusion gene EWSR1::ATF1. In this study, we performed a high-throughput drug screening, finding that the histone deacetylase inhibitor vorinostat exerted an antiproliferation effect with the reduced expression of EWSR1::ATF1. We expected the reduced expression of EWSR1::ATF1 to be due to the alteration of chromatin accessibility; however, assay for transposase-accessible chromatin using sequencing and a cleavage under targets and release using nuclease assay revealed that chromatin structure was only slightly altered, despite histone deacetylation at the EWSR1::ATF1 promoter region. Alternatively, we found that vorinostat treatment reduced the level of BRD4, a member of the bromodomain and extraterminal motif protein family, at the EWSR1::ATF1 promoter region. Furthermore, the BRD4 inhibitor JQ1 downregulated EWSR1::ATF1 according to Western blotting and qPCR analyses. In addition, motif analysis revealed that vorinostat treatment suppressed the transcriptional factor SOX10, which directly regulates EWSR1::ATF1 expression and is involved in CCS proliferation. Importantly, we demonstrate that a combination therapy of vorinostat and JQ1 synergistically enhances antiproliferation effect and EWSR1::ATF1 suppression. These results highlight a novel fusion gene suppression mechanism achieved using epigenetic modification agents and provide a potential therapeutic target for fusion gene–related tumors.

Significance:
This study reveals the epigenetic and transcriptional suppression mechanism of the fusion oncogene EWSR1::ATF1 in clear cell sarcoma by histone deacetylase inhibitor treatment as well as identifying SOX10 as a transcription factor that regulates EWSR1::ATF1 expression.
Left Atrial Function Predicts Atrial Arrhythmia Recurrence Following Ablation of Long-Standing Persistent Atrial Fibrillation
Authors: Khan Habib Rehman , Yakupoglu Haci Yakup , Kralj-Hans Ines , Haldar Shouvik , Bahrami Toufan , Clague Jonathan , De Souza Anthony , Hussain Wajid , Jarman Julian , Jones David Gareth , Salukhe Tushar , Markides Vias , Gupta Dhiraj , Khattar Rajdeep , Wong Tom ,   on behalf of the CASA AF Investigators
Published: June 2023

Background:
Left atrial (LA) function following catheter or surgical ablation of de-novo long-standing persistent atrial fibrillation (AF) and its impact on AF recurrence was studied in patients participating in the CASA-AF trial (Catheter Ablation vs. Thoracoscopic Surgical Ablation in Long Standing Persistent Atrial Fibrillation).

Methods:
All patients underwent echocardiography preablation, 3 and 12 months post-ablation. LA structure and function were assessed by 2-dimensional volume and speckle tracking strain measurements of LA reservoir, conduit, and contractile strain. Left ventricular diastolic function was measured using transmitral Doppler filling velocities and myocardial tissue Doppler velocities to derive the e’, E/e’, and E/A ratios. Continuous rhythm monitoring was achieved using an implantable loop recorder.

Results:

Eighty-three patients had echocardiographic data suitable for analysis. Their mean age was 63.6±9.7 years, 73.5% were male, had AF for 22.8±11.6 months, and had a mean LA maximum volume of 48.8±13.8 mL/m
2
. Thirty patients maintained sinus rhythm, and 53 developed AF recurrence. Ablation led to similar reductions in LA volumes at follow-up in both rhythm groups. However, higher LA emptying fraction (36.3±10.6% versus 27.9±9.9%;
P
<0.001), reservoir strain (22.6±8.5% versus 16.7±5.7%;
P
=0.001), and contractile strain (9.2±3.4% versus 5.6±2.5%;
P
<0.001) were noted in the sinus rhythm compared with AF recurrence group following ablation at 3 months. Diastolic function was better in the sinus rhythm compared with the AF recurrence group with an E/A ratio of 1.5±0.5 versus 2.2±1.2 (
P
<0.001) and left ventricular E/e’ ratio of 8.0±2.1 versus 10.3±4.1 (
P
<0.001), respectively. LA contractile strain at 3 months was the only independent predictor of AF recurrence.

Conclusions:
Following ablation for long-standing persistent AF, improvement in LA function was greater in those who maintained sinus rhythm. LA contractile strain at 3 months was the most important determinant of AF recurrence following ablation.

Registration:

URL:
https://www.clinicaltrials.gov
; Unique identifier: NCT02755688
Possessing Enlightenment: Sorcery, Selfhood, and Tragic Responsibility in a Chinese Buddhist Apocryphon
Authors: Buckelew Kevin 
Published: June 2023

Abstract
This article explores how the Lengyan jing, or Śūrangama Sūtra – an apocryphal Buddhist scripture written in China around 705 CE – remapped Chinese Buddhist understandings of moral responsibility in consequential ways. Although grounded in the orthodox doctrinal premise that all sentient beings innately possess buddha-nature, the Lengyan jing is punctuated by warnings about the danger that even the most earnest seekers of enlightenment might be possessed by demons, embark on evil behavior, and end up fully demonic. Such warnings depart from longstanding norms in Buddhist ethics, according to which responsibility for fault is measured in terms of a person’s intentions. Instead, I argue that the Lengyan jing articulates a moral logic of what Sandra Macpherson calls “tragic responsibility.” This logic informed important but overlooked aspects of the soteriological vision found in key texts from the Chan (Japanese Zen) tradition, which rose to prominence in the centuries following the Lengyan jing’s composition.
Queer Critical Media Literacy and Possibilities for Counterhegemonic Pedagogy
Authors: Woolley Susan W. 
Published: June 2023

Abstract
In this paper, the author considers the analysis of representation and the production of media as two generative pedagogical practices in which queer critical media literacy can give students tools to read and produce society and texts in counterhegemonic ways. Queering critical media literacy generates theoretical and practice-oriented interventions that can focus students’ and teachers’ attention on representation and production, including the reproduction of cisnormativity and ableism as well as their critiques. Engaging with multimodal texts through queer critical media literacy affords students and teachers creative possibilities for reading and producing queer texts, for queering the form of a text, and for queering the genre of “assignment.” Such creative production skills and critical thinking skills serve students for a lifetime while nurturing their processes of identification, representation, and critique – powerful counterhegemonic tools that can be useful in response to anti-queer sociopolitical tensions in public education.
“I Want to Know What People are Saying”: Trans Youth Learning Critical Media Literacies to Negotiate Hate on Social Media
Authors: Shrodes Addie , Coyne Claire , Chen Diane , Macapagal Kathryn , Yuodsnukis Briahna 
Published: June 2023

Abstract
We draw on 37 interviews with a racially diverse group of 21 trans and queer teenagers to consider how they used social media to navigate dominant ideologies amid rapidly accelerating white supremacy, anti-Blackness, xenophobia, anti-transness, and anti-queerness in 2019-2020. The analysis emerges from a humanizing qualitative study of lgbtq+ youth digital life conducted in partnership with an interdisciplinary gender health program that provides holistic services. Employing grounded theory and intersectional theories of queer politics, we expand conceptualizations of queer critical media literacies. We find that youth in the study enacted critical media literacies to 1) anticipate the logics of hate by which they are oppressed; 2) unlearn dominant ideologies that structure privilege; 3) and rehearse responses to intersecting oppression. Understanding the ways youth negotiate dominant ideologies on social media can inform the design of critical media literacy teaching and learning to confront phobic forces in multiple youth-serving contexts.
Creating Relating Stories: Fragmentating the Social Fabric of Heteronormativity through Critical Media Literacies
Authors: Vicars Mark , Tartakover Sarah 
Published: June 2023

Abstract
The problematics of social and academic participation for gender diverse and lgbtqi+ youth in school communities is highlighted by the stigmatising relational dynamics which often become the dominant narrative in educational domains. This paper discusses the creation of a critical literacy/media product which arose from a year 7 level curriculum project in which students were invited to seek solutions to address a problem about which they cared. In 2021 a group of Australian secondary school students undertook an investigation into gender diversity and lgbtqi+ inclusion. In partnership with teachers and pre-service teachers they co-designed an animation on gender identity opening up wider discussion of ‘orientations and dispositions’ and generating critique around issues of social justice, democracy, equality and inclusion in and out of the classroom.
Policies on doctors’ declaration of interests in medical organisations: a thematic analysis
Authors: Tzortziou Brown Victoria , McCartney Margaret , Talaga Patrycja , Huxtable Richard , Papanikitas Andrew , David-Barrett Elizabeth 
Published: June 2023

Objectives There has been growing concern about doctors’ conflicts of interests (COIs) but it is unclear what processes and tools exist to enable the consistent declaration and management of such interests. This study mapped existing policies across a variety of organisations and settings to better understand the degree of variation and identify opportunities for improvement. Design Thematic analysis. Setting We studied the COI policies of 31 UK and international organisations which set or influence professional standards or engage doctors in healthcare commissioning and provision settings. Participants 31 UK and international organisations. Main outcome measures Organisational policy similarities and differences. Results Most policies (29/31) referred to the need for individuals to apply judgement when deciding whether an interest is a conflict, with just over half (18/31) advocating a low threshold. Policies differed on the perception of frequency of COI, the timings of declarations, the type of interests that needed to be declared, and how COI and policy breaches should be managed. Just 14/31 policies stated a duty to report concerns in relation to COI. Only 18/31 policies advised COI would be published, while three stated that any disclosures would remain confidential. Conclusions The analysis of organisational policies revealed wide variation in what interests should be declared, when and how. This variation suggests that the current system may not be adequate to maintain a high level of professional integrity in all settings and that there is a need for better standardisation that reduces the risk of errors while addressing the needs of doctors, organisations and the public.
Choice of intrapleural fibrinolytic agents in the treatment of adult complicated parapneumonic effusion and empyema: Network meta-analysis
Authors: Yokoyama Yujiro , Kuno Toshiki , Takagi Hisato , Burfeind William 
Published: June 2023

Background The appropriate use of intrapleural fibrinolytic agents in patients with complicated parapneumonic effusion and empyema remains unclear, especially regarding the choice of fibrinolytic agents. We conducted a network meta-analysis comparing outcomes of intrapleural fibrinolytic agents in patients with complicated parapneumonic effusion and empyema. Methods MEDLINE and EMBASE were searched through April 2022 to identify randomized controlled trials (RCTs) that investigated outcomes in patients with complicated parapneumonic effusion or empyema who were treated with intrapleural fibrinolytic agents. The outcomes of interest were surgical requirements, bleeding, length of hospital stay, and all-cause mortality. Results Our analysis included 10 RCTs that enrolled 1085 patients treated with intrapleural tissue plasminogen activator (TPA) ( n = 138), TPA + deoxyribonuclease (DNase) ( n = 52), streptokinase ( n = 311), urokinase ( n = 75), DNase ( n = 51), or placebo ( n = 458). The rates of surgical requirement were significantly lower with TPA and TPA + DNase than with placebo (risk ratio [RR]; 95% confidence interval [CI] = 0.36 [0.14–0.97], p = 0.038, RR [95% CI] = 0.25 [0.08–0.78], p = 0.017, respectively). The risk of bleeding was higher with TPA + DNase than with placebo (RR [95% CI] = 10.91 [1.53–77.99], p = 0.017), as well as TPA and TPA + DNase than with urokinase (RR [95% CI] = 17.90 [1.07–299.44], p = 0.044, RR [95% CI] = 89.3 [2.88–2772.49], p = 0.010, respectively). All-cause mortality was similar among the groups. Conclusion TPA and TPA + DNase reduced the rates of surgical requirement compared with placebo. However, TPA + DNase increased the risk of bleeding compared with placebo. Intrapleural agents for complicated parapneumonic effusion and empyema should be selected with an individual risk assessment.
Is it feasible to enhance quality assurance of cervical cancer screening in Latin America? A regional expert consensus
Authors: Rangel Valentina , Fernández-Deaza Ginna Paola , Castillo Juan Sebastián , Murillo Raúl 
Published: June 2023

Objectives Cervical cancer elimination requires high-performance screening tests and high treatment rates, and thus high screening program performance is essential; however, Latin America lacks organized screening and quality assurance (QA) guidelines. We aimed to develop a core set of QA indicators suitable to the region. Methods We reviewed QA guidelines from countries/regions with highly organized screening programs and selected 49 indicators for screening intensity, test performance, follow-up, screening outcomes and system capacity. A regional expert consensus using the Delphi method in two rounds was implemented to identify basic indicators actionable within the regional context. The panel was integrated by recognized Latin American scientists and public health experts. They voted for the indicators blinded to each other based on feasibility and relevance. The correlation between both attributes was analyzed. Results In the first round 33 indicators reached consensus for feasibility but only 9 for relevance, without full coincidence. In the second round 9 indicators met the criteria for both (2 screening intensity, 1 test performance, 2 follow-up, 3 outcomes, 1 system capacity). A significant positive correlation was observed for test performance and outcomes indicators between the two attributes assessed ( p < 0.05). Conclusions Cervical cancer control requires realistic goals supported by proper programs and QA systems. We identified a set of indicators suitable to improve cervical cancer screening performance in Latin America. The assessment by an expert panel with a joint vision from science and public health practice represents a significant progress towards real and feasible QA guidelines for countries in the region.
Avatar and virtual agent-assisted telecare for patients in their homes: A scoping review
Authors: Winkler Anna , Kutschar Patrick , Pitzer Stefan , van der Zee-Neuen Antje , Kerner Susanne , Osterbrink Jürgen , Krutter Simon 
Published: June 2023

Introduction Telecare can be an effective way to deliver healthcare to patients’ homes. Avatar or virtual agent-equipped technologies have the potential to increase user engagement and adherence to telecare. This study aimed to identify telecare interventions assisted by avatars/virtual agents, reflect the concepts of telecare and give an overview on its outcomes. Methods A scoping review guided by the PRISMA-ScR checklist was conducted. MEDLINE, CINAHL, PsycINFO and grey literature were searched through 12 July 2022. Studies were included if patients were remotely cared for by healthcare professionals and received telecare interventions assisted by avatars/virtual agents in their homes. Studies underwent quality appraisal, and were synthesized along the dimensions ‘study characteristics’, ‘intervention’ and ‘outcomes’. Results Out of 535 records screened, 14 studies were included, reporting the effects of avatar/virtual agent-assisted telecare interventions, tailored to specific patient groups. Telecare interventions mainly focused on teletherapy and telemonitoring. Telecare services were rehabilitative, preventive, palliative, promotive and curative. Modes of communication were asynchronous, synchronous or a mix of both. Tasks of the implemented avatars/virtual agents comprised delivering health interventions, monitoring, assessment, guidance and strengthening agency. Telecare interventions led to improved clinical outcomes and higher adherence. Most studies reported sufficient system usability and high satisfaction among participants. Conclusions Telecare interventions were overall target group related and integrated in a service model. This combined with the use of avatars and virtual agents leads to improved adherence to telecare in the home setting. Further studies could account for relatives’ experiences with telecare.
Cognitive Impairments After Stroke Do Not Attenuate Explicit Visuomotor Adaptation in Reaching and Savings With the Unaffected Arm
Authors: Binyamin-Netser Reut , Goldhamer Noy , Avni Inbar , Ressel Zviely Adva , Shmuelof Lior 
Published: June 2023

Background Accumulating evidence suggests that motor performance is affected by the cognitive control abilities of the subject. Performance in motor tasks in populations with cognitive deficits such as older adults and subjects with stroke is therefore expected to deteriorate. The goal of this study is to investigate the relationship between cognitive impairments and motor control and learning impairments in a visuomotor adaptation task in subjects with stroke. Methods Twenty-seven post-stroke, 31 age matched controls, and 30 young control subjects completed a sensorimotor adaptation task composed of 2 adaptation blocks separated by a washout block. Explicit learning was assessed by cueing subjects to suppress their strategy. Cognitive assessment was conducted using the Montreal Cognitive Assessment (MoCA) and a verbal learning test. Subjects with stroke performed the task with their unaffected arm. Results Despite the cognitive deterioration, the adaptation and savings of the stroke group and age matched controls were comparable. Adaptation and savings were smaller with respect to the young subjects. Savings was associated with a significant improvement in the explicit component across blocks. Finally, the explicit enhancement between blocks was significantly correlated with the MoCA scores in the stroke group and with the results of the verbal learning test in the young controls. Conclusion The lack of stroke-induced attenuation on adaptation, despite a correlation between cognitive abilities and explicit learning in adaptation, suggests that subjects with stroke have enough cognitive resources to support sensorimotor adaptation. The availability of cognitive resources for motor learning following brain damage could be utilized in the rehabilitation process.
Color and NICU Design
Authors: Shepley Mardelle McCuskey , Ames Rebecca L. , Lin Connie Y. 
Published: June 2023

Purpose: The appropriate use of color in healthcare settings has been a topic of interest for designers and researchers, and the need for evidence-based standards evident. The purpose of this article is to summarize recent research on color as applicable to neonatal intensive care units and to propose standards for color in these settings. Background: Research on this topic is limited due to difficulties associated with constructing research protocols, challenges in setting parameters for the independent variable (color), and the need to simultaneously address infants, families, and caregivers. Methods: For our literature review, the following research question was developed: Does the use of color in the design of the neonatal intensive care units (NICUs) impact health outcomes in newborn infants, families, and/or staff? Using Arksey and O’Malley’s framework for conducting a structured literature review, we (1) identified the research question, (2) identified relevant studies, (3) selected studies, and (4) collated and summarized the results. Only four papers were found regarding NICUs, so the search was expanded to include related healthcare and authors reporting on best practice. Results: Overall, the primary research focused on behavioral or physiological outcomes including the role of wayfinding and art, the impact of lighting on color, and tools for evaluating the impact of color. Best practice recommendations sometimes reflected the primary research but occasionally provided contradictory advice. Conclusions: Based on the reviewed literature, five topics are addressed: palette malleability; the use of the primary colors, blue, red, and yellow; and the relationship between light and color.
Exhaled volatile organic compounds associated with risk factors for obstructive pulmonary diseases: a systematic review
Authors: Shahbazi Khamas Shahriyar , Alizadeh Bahmani Amir Hossein , Vijverberg Susanne J.H. , Brinkman Paul , Maitland-van der Zee Anke H. 
Published: June 2023

BackgroundAsthma and COPD are among the most common respiratory diseases. To improve the early detection of exacerbations and the clinical course of asthma and COPD new biomarkers are needed. The development of noninvasive metabolomics of exhaled air into a point-of-care tool is an appealing option. However, risk factors for obstructive pulmonary diseases can potentially introduce confounding markers due to altered volatile organic compound (VOC) patterns being linked to these risk factors instead of the disease. We conducted a systematic review and presented a comprehensive list of VOCs associated with these risk factors.MethodsA PRISMA-oriented systematic search was conducted across PubMed, Embase and Cochrane Libraries between 2000 and 2022. Full-length studies evaluating VOCs in exhaled breath were included. A narrative synthesis of the data was conducted, and the Newcastle–Ottawa Scale was used to assess the quality of included studies.ResultsThe search yielded 2209 records and, based on the inclusion/exclusion criteria, 24 articles were included in the qualitative synthesis. In total, 232 individual VOCs associated with risk factors for obstructive pulmonary diseases were found; 58 compounds were reported more than once and 12 were reported as potential markers of asthma and/or COPD in other studies. Critical appraisal found that the identified studies were methodologically heterogeneous and had a variable risk of bias.ConclusionWe identified a series of exhaled VOCs associated with risk factors for asthma and/or COPD. Identification of these VOCs is necessary for the further development of exhaled metabolites-based point-of-care tests in these obstructive pulmonary diseases.