bluuebunny/crossref_metadata_2025_split huggingface dataset:
Broad organ acceptance and equitable access to early kidney transplantation
Authors: Zaphiros Nikolas H. , Nie Jing , Chang Shirley , Shah Vaqar , Kareem Samer , Zaaroura Ahmad , Kayler Liise K. 
Published: January 2023

AbstractBackgroundBroad organ acceptance can increase early kidney transplantation (KTX) within <1‐year of dialysis initiation while improving access inequity.MethodsSingle‐center data of adult isolated deceased‐donor KTX recipients between 2013 and 2020 were stratified into three 2.5‐year periods before‐, early after‐, and late after our center's deceased‐donor organ acceptance practice change, excluding a 6‐month implementation period. Outcomes were assessed within five recipient subgroups based on demographic and clinical characteristics.ResultsOf 704 recipients, the frequency of early KTX was 22% pre‐change, 36% early post‐change, and 34% late post‐change. Given similar post‐change frequencies of early KTX, post‐change eras were combined to improve analytic power of subgroup analyses. After the organ acceptance practice change (vs. pre‐change), the likelihood of early KTX increased variably within historically underserved groups, including recipients who were older (37%–39%, p = .859), Black (10%–21%, p = .136), female (21%–37%, p = .034), diabetic (13%–32%, p = .010), and BMI≥35 kg/m2 (20%–34%, p = .007). Despite the practice change, Black recipients continued to experience less early KTX compared to non‐Black recipients. The likelihood of delayed graft function was significantly increased (p < .001), and 1‐year creatinine was significantly higher (p < .001) post‐practice change, but between‐era risk‐adjusted death‐censored graft survival was similar.ConclusionsTransition to broader donor acceptance was associated with more early KTXs among historically underserved patient subgroups. However, the effect was non‐significant among Black recipients, suggesting the need for additional strategies to impact early transplant access for this population. Studies of broad organ acceptance are needed to examine both access and outcomes.
Synchronous occurrence of primary cutaneous B‐cell lymphoma and cutaneous Rosai–Dorfman disease in distinct lesions: A unique association
Authors: Roccuzzo Gabriele , Avallone Gianluca , Cavallo Francesco , Mastorino Luca , Conti Luca , Fava Paolo , Tomasini Carlo , Ribero Simone , Quaglino Pietro 
Published: January 2023

AbstractRosai–Dorfman disease, also known as sinus histiocytosis with massive lymphadenopathy (SHML), is a rare subtype of reactive histiocytosis which is seldom associated with Hodgkin's and non‐Hodgkin's lymphomas. To date, the coexistence in the same patient of extra nodal SHML and primary cutaneous B‐cell lymphoma (PCBCL) has been reported in the literature, as metachronous diagnosis in the anatomical area of the original PCBCL or synchronous occurrence in the same lesions. However, no data have been published as for synchronous occurrence of the two pathological entities in distinct anatomical sites. Herein, we report the first ever described synchronous occurrence of PCBCL and SHML, detected in distinct lesions, affecting the same patient. The complete resolution of the patient's PCBCL after rituximab treatment and the concomitant regression of SHML suggest that this clinically benign reactive histiocytic proliferation, potentially triggered by the lymphoma microenvironment itself, may take place not only in the site of the PCBCL lesion, but also in other distant areas not directly affected by the primary cutaneous lymphoma.
Motor function in early onset schizophrenia—A 2‐year follow‐up study
Authors: Byrial Pernille , Nyboe Lene , Thomsen Per Hove , Clausen Loa 
Published: January 2023

AbstractAimMotor symptoms primarily assessed by clinical rating are documented across the schizophrenia spectrum, but no studies have examined the longitudinal course of these symptoms in adolescents using tests that control for the natural maturational process. The aim is therefore to compare fine and gross motor function using age‐adjusted tests in adolescents with schizophrenia and controls across a 2‐year period, and examine if clinical correlates contribute to changes in motor function in adolescents with schizophrenia.MethodMotor function assessed by two age‐adjusted tests was compared in 25 adolescents with schizophrenia and age‐ and sex‐matched controls over a 2‐year period using t‐tests, Cohen's D and χ2 tests. Linear mixed models with a random intercept at patient level were used to assess changes between baseline and follow‐up. The latter approach was adopted to assess the association between changes and potential predictors as age, sex, complications during labour/delivery, childhood motor function, symptoms severity, executive function and antipsychotics.ResultAll measures of motor function but one significantly differentiated adolescents with schizophrenia from controls with large effect sizes at 2‐year follow‐up. The overall scores did not change during follow‐up, whereas two resembling motor areas of the tests significantly improved in adolescents with schizophrenia. The severity of schizophrenia, sex and IQ revealed association with the changes.ConclusionThe finding of both stability and improvements from diagnosis to follow‐up in adolescents with schizophrenia and the differences between adolescents with and without schizophrenia argue in favour of the neurodevelopment hypothesis and highlights the need for assessing motor function.
An ecological assessment of Australia's first community oyster gardens
Authors: Boström‐Einarsson Lisa , Martínez‐Baena Francisco , Diggles Ben , Firby Lauren , McLeod Ian M. 
Published: January 2023

SummaryOyster gardening is a community‐driven activity where oysters are grown in cages hanging off docks or other coastal infrastructure. Besides the provision of adult oysters for restoration programmes, oyster gardening may also support other ecosystem services such as providing habitat for fishes and invertebrates as well as encouraging community involvement and citizen science. Australia's first oyster gardening programme was undertaken in a canal estate on Bribie Island in Moreton Bay, Queensland between October 2016 and November 2017. Oyster gardens consisting of plastic mesh cages were deployed with either three species of bivalves (polyculture), or exclusively Sydney Rock Oysters (monoculture) to investigate whether the habitat value differed between the two garden types. After one year of growth, polyculture cages supported higher abundances and species richness of both invertebrates and fish compared to the monoculture gardens. Our study showed that oyster gardening can provide habitat for a range of invertebrate and fish species in the highly modified coastal environment of a canal estate. Further studies are needed to discern whether these oyster gardens would also support larger and mobile fauna, such as species with commercial and recreational importance.
Impact of PIEZO1‐channel on inflammation and osteoclastogenesis mediated via periodontal ligament fibroblasts during mechanical loading
Authors: Schröder Agnes , Neher Katharina , Krenmayr Bernhard , Paddenberg Eva , Spanier Gerrit , Proff Peter , Kirschneck Christian 
Published: January 2023

AbstractThe identification of mechanosensitive ion channels and their importance in innate immunity provides new starting points to elucidate the molecular mechanisms of orthodontic tooth movement. The mechanosensitive electron channel PIEZO1 (Piezo Type Mechanosensitive Ion Channel Component 1) may play a crucial role in orthodontic tooth movement. To investigate the role of the PIEZO1 channel, periodontal ligament fibroblasts (PDLF) were subsequently treated with a PIEZO1 inhibitor (GsMTx) with simultaneous pressure application or with an activator (JEDI2) without mechanical strain. The expression of genes and proteins involved in orthodontic tooth movement was examined by RT‐qPCR, Western blot and ELISA. In addition, the effect on PDLF‐mediated osteoclastogenesis was investigated in a coculture model using human monocytes. Inhibition of PIEZO1 under pressure application caused a reduction in RANKL (receptor activator of NF‐kB ligand) expression, resulting in decreased osteoclastogenesis. On the other hand, activation of PIEZO1 without mechanical strain downregulated OPG (osteoprotegerin), resulting in increased osteoclastogenesis. PIEZO1 appears to play a role in the induction of inflammatory genes. It was also shown to influence osteoclastogenesis.
Gain of function SCN1A disease‐causing variants: Expanding the phenotypic spectrum and functional studies guiding the choice of effective antiseizure medication
Authors: Matricardi Sara , Cestèle Sandrine , Trivisano Marina , Kassabian Benedetta , Leroudier Nathalie , Vittorini Roberta , Nosadini Margherita , Cesaroni Elisabetta , Siliquini Sabrina , Marinaccio Cristina , Longaretti Francesca , Podestà Barbara , Operto Francesca Felicia , Luisi Concetta , Sartori Stefano , Boniver Clementina , Specchio Nicola , Vigevano Federico , Marini Carla , Mantegazza Massimo 
Published: January 2023

AbstractObjectiveThis study was undertaken to refine the spectrum of SCN1A epileptic disorders other than Dravet syndrome (DS) and genetic epilepsy with febrile seizures plus (GEFS+) and optimize antiseizure management by correlating phenotype–genotype relationship and functional consequences of SCN1A variants in a cohort of patients.MethodsSixteen probands carrying SCN1A pathogenic variants were ascertained via a national collaborative network. We also performed a literature review including individuals with SCN1A variants causing non‐DS and non‐GEFS+ phenotypes and compared the features of the two cohorts. Whole cell patch clamp experiments were performed for three representative SCN1A pathogenic variants.ResultsNine of the 16 probands (56%) had de novo pathogenic variants causing developmental and epileptic encephalopathy (DEE) with seizure onset at a median age of 2 months and severe intellectual disability. Seven of the 16 probands (54%), five with inherited and two with de novo variants, manifested focal epilepsies with mild or no intellectual disability. Sodium channel blockers never worsened seizures, and 50% of patients experienced long periods of seizure freedom. We found 13 SCN1A missense variants; eight of them were novel and never reported. Functional studies of three representative variants showed a gain of channel function. The literature review led to the identification of 44 individuals with SCN1A variants and non‐DS, non‐GEFS+ phenotypes. The comparison with our cohort highlighted that DEE phenotypes are a common feature.SignificanceThe boundaries of SCN1A disorders are wide and still expanding. In our cohort, >50% of patients manifested focal epilepsies, which are thus a frequent feature of SCN1A pathogenic variants beyond DS and GEFS+. SCN1A testing should therefore be included in the diagnostic workup of pediatric, familial and nonfamilial, focal epilepsies. Alternatively, non‐DS/non‐GEFS+ phenotypes might be associated with gain of channel function, and sodium channel blockers could control seizures by counteracting excessive channel function. Functional analysis evaluating the consequences of pathogenic SCN1A variants is thus relevant to tailor the appropriate antiseizure medication.
Circ_0001402 knockdown suppresses the chemoresistance and development of DDP‐resistant cutaneous squamous cell carcinoma cells by functioning as a ceRNA for miR‐625‐5p
Authors: Li Min , Luo Ming , Liu Pei , Wang Runchao , Jing Haixia 
Published: January 2023

AbstractCutaneous squamous cell carcinoma (CSCC) is the most common metastatic skin cancer. Circular RNAs (circRNAs) are differentially expressed in CSCC and can sequester and sponge microRNAs. GSE74758 shows that hsa_circ_0001402 (circ_0001402) is the most overexpressed circRNA in CSCC. Expression of circ_0001402, microRNA(miR)‐625‐5p and karyopherin subunit alpha 4 (KPNA4) was detected by quantitative real‐time polymerase chain reaction and/or Western blot. Colon formation, flow cytometry, Transwell assays and xenograft tumour model confirmed the development of CSCC cells. The competing endogenous RNA (ceRNA) interaction was confirmed by dual‐luciferase reporter assay and RNA immunoprecipitation (RIP) assay. Circ_0001402 was significantly upregulated in CSCC tissues and cells, and higher expression of circ_0001402 was found in DDP‐resistant samples. Functionally, circ_0001402 knockdown induced apoptosis and inhibited half maximal inhibitory concentration of DDP, colony formation, migration and invasion of DDP‐resistant CSCC cells, accompanied with the depressed multi‐drug resistance‐1 (MDR1) and MDR‐related protein‐1, while miR‐625‐5p inhibitor could counteract these effects. Mechanically, circ_0001402 mediated the expression regulation of KPNA4 via functioning as a ceRNA for miR‐625‐5p. KPNA4 re‐expression could abate the functions of miR‐625‐5p. Furthermore, circ_0001402 knockdown could hinder tumour growth of DDP‐resistant CSCC. Circ_0001402 knockdown can suppress the development and chemoresistance of DDP‐resistant CSCC cells at least partly through targeting miR‐625‐5p/KPNA4 axis.
Novel Miscanthus hybrids: Modelling productivity on marginal land in Europe using dynamics of canopy development determined by light interception
Authors: Shepherd Anita , Awty‐Carroll Danny , Kam Jason , Ashman Chris , Magenau Elena , Martani Enrico , Kontek Mislav , Ferrarini Andrea , Amaducci Stefano , Davey Chris , Jurišić Vanja , Petrie Gert‐Jan , Al Hassan Mohamad , Lamy Isabelle , Lewandowski Iris , de Maupeou Emmanuel , McCalmont Jon , Trindade Luisa , van der Cruijsen Kasper , van der Pluijm Philip , Rowe Rebecca , Lovett Andrew , Donnison Iain , Kiesel Andreas , Clifton‐Brown John , Hastings Astley 
Published: January 2023

AbstractNew biomass crop hybrids for bioeconomic expansion require yield projections to determine their potential for strategic land use planning in the face of global challenges. Our biomass growth simulation incorporates radiation interception and conversion efficiency. Models often use leaf area to predict interception which is demanding to determine accurately, so instead we use low‐cost rapid light interception measurements using a simple laboratory‐made line ceptometer and relate the dynamics of canopy closure to thermal time, and to measurements of biomass. We apply the model to project the European biomass potentials of new market‐ready hybrids for 2020–2030. Field measurements are easier to collect, the calibration is seasonally dynamic and reduces influence of weather variation between field sites. The model obtained is conservative, being calibrated by crops of varying establishment and varying maturity on less productive (marginal) land. This results in conservative projections of miscanthus hybrids for 2020–2030 based on 10% land use conversion of the least (productive) grassland and arable for farm diversification, which show a European potential of 80.7–89.7 Mt year−1 biomass, with potential for 1.2–1.3 EJ year−1 energy and 36.3–40.3 Mt year−1 carbon capture, with seeded Miscanthus sacchariflorus × sinensis displaying highest yield potential. Simulated biomass projections must be viewed in light of the field measurements on less productive land with high soil water deficits. We are attempting to model the results from an ambitious and novel project combining new hybrids across Europe with agronomy which has not been perfected on less productive sites. Nevertheless, at the time of energy sourcing issues, seed‐propagated miscanthus hybrids for the upscaled provision of bioenergy offer an alternative source of renewable energy. If European countries provide incentives for growers to invest, seeded hybrids can improve product availability and biomass yields over the current commercial miscanthus variety.
Interleukin‐3‐dependent potentiation of IgE responsiveness in mouse basophils
Authors: Kitano Takuma , Togawa Kaho , Takemori Juri , Motoki Yuya , Kishida Keitaroh , Itoh Saotomo , Takamoto Masaya , Taki Shinsuke , Hida Shigeaki 
Published: January 2023

AbstractBasophils produce interleukins (IL)‐4 in response to various stimuli and may contribute to type 2 immune responses to various infections and allergens. We found that resting basophils freshly isolated from mice produce IL‐4 in response to IL‐3 but not to high‐affinity Fc receptor (FcεRI) cross‐linking (CL), yet both required the immunoreceptor tyrosine‐based activation motif (ITAM) containing adaptor Fc receptor γ‐chain (FcRγ), while basophils activated in vitro by IL‐3 become responsive to FcεRI CL. Acquisition of responsiveness to FcεRI CL occurred upon infection with Trichinella spiralis or administration of superantigen. Because cultured basophils return to a quiescent state upon starvation with IL‐3 with surface FcεRI levels unchanged, this acquisition is reversible and probably reflects intracellular events requiring protein synthesis. Interestingly, similar activation‐associated acquisition was observed for responsiveness to other stimuli, including CD200R3 CL, which is known to signal via DAP‐12, and the allergen protease papain. This acquisition of responsiveness to FcεRI CL was inhibited by Jak inhibitor. Thus, the IL‐3 signal bifurcates downstream of Jak, into two distinct pathway, one leading to IL‐4 production and the other to render basophils competent to respond to stimuli dependent on ITAM‐containing adaptors DAP12 and FcRγ for IL‐4 production.
Preparation of nano‐AlN powder by sol–gel foaming and its sintering properties
Authors: Liu Mengyang , Wang Shuangshuang , Li Sifang , Liu Chengwei , Luo Shuaiou , Wang Deqiang 
Published: January 2023

AbstractUniformly dispersed nano‐sized aluminum nitride powders were prepared by the sol–gel foaming method using aluminum nitrate as the aluminum source, sucrose as the carbon source, and ammonium chloride as the foaming agent. The effects of ammonium chloride content on the particle size and the sintering properties of aluminum nitride were investigated. The results showed that when the molar ratio of ammonium chloride to aluminum nitrate was .5, the colloidal foams were uniform, large, and fluffy, and amorphous alumina precursors with uniform particles could be prepared. Aluminum nitride powder with a particle size of 22–27 nm can be obtained by calcining these precursors in nitrogen atmosphere at 1400°C for 2 h. At the same time, aluminum nitride bulk material with a relative density of 95% can be obtained by sintering the compact samples in nitrogen atmosphere at 1700°C for 2 h.
Hospital triage and skin disease: hospital outcomes are differentially associated with cutaneous morphology
Authors: Pettit Cory , Trinidad John , Chung Catherine , Patterson Andrew , Kaffenberger Benjamin H. 
Published: January 2023

AbstractBackgroundDetermining the exact dermatologic diagnosis is difficult in the inpatient setting.ObjectiveDetermine whether morphologic classification rather than specific diagnosis is associated with hospital outcomes.MethodsRetrospective single‐center study. Information from 1798 inpatient dermatology consults at The Ohio State University Wexner Medical Center from 2012 to 2014 was queried. Dermatologic diseases were categorized into 16 groups based on appearance. Logistic regression was performed comparing mortality rate vs morphology. Linear regression was performed comparing the length of stay (LOS) vs morphology.ResultsMorphology was associated with a mortality rate (P = 0.038). The morphologic subgroups acneiform/follicular/occlusion (P = 0.011), blistering disorders (P = 0.009), retiform purpura (P = 0.011), and vasculitis/vascular (P = 0.007) were associated with increased mortality. Morphology was associated with LOS (P = 0.004), and the morbilliform subgroup was associated with increased LOS (P < 0.001).ConclusionThis study demonstrated the importance of morphologic diagnosis and its association with mortality rate and LOS. This information may help triage cutaneous disorders in the inpatient setting and determine the relative risk of dermatologic conditions when assessing the need for hospital transfers and more aggressive therapies.
Prophylaxis effects of water kefir on post‐infectious irritable bowel syndrome in rat model
Authors: Gökırmaklı Çağlar , Erol Zeki , Gun Ilhan , Ozmen Ozlem , Guzel‐Seydim Zeynep Banu 
Published: January 2023

SummaryWater kefir (WK) is produced using WK grains containing beneficial bacteria and yeasts. It has antibacterial, anti‐inflammatory, anti‐diabetic, anti‐hyperlipidaemic, anti‐ulcerogenic, and antioxidant effects. The purpose of the research was to determine the effect of WK on irritable bowel syndrome (IBS). In this study, thirty‐two female Wistar‐Albino rats aged 3 months were randomly distributed to four groups: the control group (C), the WK group, the WK group with IBS (WK + IBS), and the IBS group. Results indicated that the IBS groups had higher white blood cell, lymphocyte, monocyte counts, and procalcitonin levels. The findings of WK + IBS group showed that WK improved against IBS. TNF‐α, NF‐kB expressions, and histopathological results significantly decreased after receiving WK treatment. In conclusion, ingestion of WK has prophylactic effects on IBS as exhibited through anin‐vivomurine model. The findings suggested that IBS may be prevented and treated with WK.
Grapefruit peel essential oil nanoemulsions: preparation, characterisation, stability and antifungal potential against Penicillium digitatum of Kinnow
Authors: Dudeja Isha , Tandon Ritu , Kalia Anu 
Published: January 2023

SummaryHydrophobicity of essential oils poses a big hindrance for harnessing their diverse antimicrobial portfolio. The study was therefore planned to develop and explore grapefruit peel essential oil nanoemulsions (GPEO‐NEs) as an eco‐friendly hydrophilic alternate against Penicillium digitatum, a prominent postharvest fungus of kinnow. GPEO‐NEs were prepared by mixing GPEO and surfactant (Tween 80) in different ratios (v/v) viz 1:0.5–1:3 by bath and probe sonication. The 1:1 OSR GPEO‐NE (20 min) by probe sonication exhibited consistent droplet diameter (96.6 nm) and PDI (0.247). All GPEO‐NEs were evaluated for antifungal potential against P. digitatum using poisoned food technique. The GPEO‐NEs (1:1 OSR; 20 min) revealed the best efficacy (88.6%) as compared to pure GPEO (76.6%). Optical microscopy further revealed stronger suppressive activity of GPEO‐NEs for spore germination and hyphal distortion of P. digitatum than pure oil. The GPEO‐NEs can thus serve as a green alternative to synthetic fungicides for managing postharvest losses in kinnow by using these as coatings.