bluuebunny/crossref_metadata_2025_split huggingface dataset:
Mechanisms underlying the antiarrhythmic effect of ARumenamide-787 in experimental models of the J wave syndromes and hypothermia
Authors: Di Diego José M. , Barajas-Martinez Hector , Cox Robert , Robinson Victoria M. , Jung Joseph , Fouda Mohamed , Patocskai Bence , Abdelsayed Mena , Ruben Peter C. , Antzelevitch Charles 
Published: May 2023

Background
Brugada (BrS) and early repolarization syndromes (ERS), the so-called J wave syndromes (JWS), are associated with life-threatening ventricular arrhythmias. Pharmacologic approaches to therapy are currently limited. In this study, we examine the effects of ARumenamide-787 (AR-787) to suppress the electrocardiographic and arrhythmic manifestations of JWS and hypothermia.

Methods
We studied the effects of AR-787 on INa and IKr in HEK-293 cells stably expressing the α- and β1-subunits of the cardiac (NaV1.5) sodium channel and hERG channel, respectively. In addition, we studied its effect on Ito, INa and ICa in dissociated canine ventricular myocytes along with action potentials and ECG from coronary-perfused right (RV) and left (LV) ventricular wedge preparations. The Ito agonist, NS5806 (5–10 μM), ICa blocker, verapamil (2.5 μM), and INa blocker, ajmaline (2.5 μM), were used to mimic the genetic defects associated with JWS and to induce the electrocardiographic and arrhythmic manifestations of JWS (prominent J waves/ST segment elevation, phase 2 reentry and polymorphic VT/VF) in canine ventricular wedge preparations.

Results
AR-787 (1, 10 and 50 μM) exerted pleiotropic effects on cardiac ion channels. The predominant effect was inhibition of the transient outward current (Ito) and enhancement of the sodium channel current (INa), with lesser effects to inhibit IKr and augment calcium channel current (ICa). AR-787 diminished the electrocardiographic J wave and prevented and/or suppressed all arrhythmic activity in canine RV and LV experimental models of BrS, ERS and hypothermia.

Conclusions
Our findings point to AR-787 as promising candidate for the pharmacologic treatment of JWS and hypothermia.
Effectiveness of multimedia education for reducing anxiety among caregivers of children and adolescents undergoing chemotherapy: Randomized controlled trial protocol
Authors: de Oliveira Silva Silmara , da Silva Duarte Fernando Hiago , de Souza Costa Thatiane Monick , de Araújo Naryllenne Maciel , Barros Leal Nahadja Tahaynara , Medeiros Kleyton Santos , Neves Dantas Rodrigo Assis , Dantas Daniele Vieira 
Published: May 2023

IntroductionChildhood cancer affects approximately 600,000 children and adolescents worldwide, and chemotherapy is the main form of treatment. However, chemotherapy treatment causes feelings of fear and anxiety especially in the patient’s caregiver. Thus, strategies that help the health education process directed towards caregivers are essential for strengthening knowledge and reducing anxiety involved with the beginning of treatment.ObjectiveTo present a study protocol to evaluate the effect of a multimedia strategy compared to standard guidelines for acquiring knowledge and reducing anxiety among caregivers of children and adolescents with cancer undergoing chemotherapy.MethodsA randomized, controlled, single-blind, two-armed clinical trial will be carried out. Fifty-two caregivers of children and adolescents who will start chemotherapy will participate in the study, which will be randomly assigned into Experimental Group, which involves the evaluation of the effect of a multimedia strategy composed of a digital animation film about the chemotherapy process, used as tool for health education or into Control Group, which assesses the effects of standard guidelines, which are verbally provided. Two important moments will be considered to evaluate the results of the intervention (P1, and F1). The primary outcome includes reduced anxiety and the secondary outcome refers to the caregivers’ acquisition of knowledge about chemotherapy treatment.Expected resultsThe results of this randomized clinical trial will have a positive impact on the participants’ knowledge acquisition, and will also contribute to reduce anxiety observed at the beginning of treatment related to the caregivers’ knowledge deficit. The level of knowledge between groups with anxiety before and after intervention will be compared, highlighting which intervention had the best effect.Evaluation recordRegistration: RBR-4wdm8q9–Brazilian Registry of Clinical Trials–REBEC (23/03/2022). This study was approved by the Research Ethics Committee of the Federal University of Rio Grande do Norte- UFRN, under CAAE—52597121.9.0000.5537.
Quantifying workload using nonlinear dynamical measures of biomechanical parameters during cycling on a roller trainer
Authors: Harsch Ann-Kathrin , Kunert Alexander , Koska Daniel , Maiwald Christian 
Published: May 2023

The aim of the present study was to determine the effectiveness of nonlinear parameters in distinguishing individual workload in cycling by using bike-integrated sensor data. The investigation focused on two nonlinear parameters: The ML1, which analyzes the geometric median in phase space, and the maximum Lyapunov exponent as nonlinear measure of local system stability. We investigated two hypothesis: 1. ML1α, derived from kinematic crank data, is as good as ML1F, derived from force crank data, at distinguishing between individual load levels. 2. Increasing load during cycling leads to decreasing local system stability evidenced by linearly increasing maximal Lyapunov exponents generated from kinematic data.
A maximal incremental cycling step test was conducted on an ergometer, generating complete datasets from 10 participants in a laboratory setting. Pedaling torque and kinematic data of the crank were recorded. ML1F, ML1α, and Lyapunov parameters (λst, λlt, ιst, ιlt) were calculated for each participant at comparable load levels. The results showed a significant linear increase in ML1α across three individual load levels, with a lower but still large effect compared to ML1F. The contrast analysis also confirmed a linearly increasing trend for λst across three load levels, but this was not confirmed for λlt. However, the intercepts ιst and ιlt of the short- and longterm divergence showed a statistically significant linear increase across the load levels.
In summary, nonlinear parameters seem fundamentally suitable to distinguish individual load levels in cycling. It is concluded that higher load during cycling is associated with decreasing local system stability. These findings may aid in developing improved e-bike propulsion algorithms. Further research is needed to determine the impact of factors occurring in field application.
Dynamic tests of the culvert face within crashtests
Authors: Nouzovský Luboš , Kohout Tomáš , Vrtal Pavel , Nováček Jakub 
Published: May 2023

Severity of a vehicle impact into solid obstacles alongside the road can be annually illustrated through accident statistics. One example of such solid obstacles is drainage ditch culvert faces. Within the research at the Faculty of Transportation Sciences of the Czech Technical University new equipment has been designed in a form of a modular system of the drainage ditch culvert faces construction. The qualities of this equipment have been tested by means of crash tests with a passenger vehicle Škoda Fabia making use of criteria of biomechanical endurance of the vehicle passengers. The results have been compared with the test carried out by means of a vehicle crash into concrete culvert faces.
The Ideology of Contemporary Islamic Education: Foundations of Thought at 3 Islamic Educational Institutions in Yogyakarta
Authors: Sodiq Ali , Siregar Maragustam , Maksudin Maksudin , Shaleh Shaleh 
Published: May 2023

Purpose – This study aims to discuss the ideological form of contemporary Islamic education in Indonesia. This study investigates the phenomenology of three Yogyakarta school institutions.
Design/methods/approach – This is a qualitative study with a phenomenological pedagogical approach. This study focuses on three Islamic educational institutions in the Special Region of Yogyakarta: Madrasah Aliyah Unggulan (MAU) Al-Imdad Bantul Yogyakarta, Madrasah Mu’allimin Muhammadiyah (M3) Yogyakarta, and SMAIT Abu Bakar Yogyakarta. Purposive sampling and snowball techniques were used to select the informants.
Findings – This study found that Al-Imdad Superior Madrasah Aliyah was an educational advancement from Nadlotul Ulama. The educational ideology of the Al-Imdad Superior Aliyah Madrasah is conservative-religious. Madrasah Mu’allimin Muhammadiyah is a Muhammadiyah cadre school with a fundamental conservatism ideology. SMAIT Abu Bakar has a fundamentalist-conservative ideology and a formalistic-revivalist religious attitude.
Research implications/limitations – This study only looks at the ideological perspectives of Islamic educational institutions. The results of this study can be used to make recommendations to policymakers, particularly the Ministry of Religion of the Republic of Indonesia, in determining the direction of future development of Islamic educational institutions. However, this study only looks at the aspect of thinking. Further research can concentrate on the managerial aspects of each Islamic educational institution.
Practical implications – The results of this study shed light on the various characteristics of Islamic educational institutions in Indonesia. In theory, this research has implications for developing an Islamic thought map regarding existing Islamic educational institutions. This study also provides insight into Indonesia’s ideological diversity of Islamic educational institutions.
STUDI TEKNO-EKONOMI PROSES PIROMETALURGI DAUR ULANG BATERAI LITHIUM MANGANESE OXIDE (LMO) DAN LITHIUM IRON PHOSPHATE (LFP)
Authors: Triaswinanti Rininta , Triastomo Rahmadhani , Puspita Angella Natalia Ghea , Hapid Abdul 
Published: May 2023

Limbah baterai lithium-ion diproyeksikan akan meningkat seiring dengan peningkatan jumlah kendaraan listrik. Teknologi daur ulang baterai menjadi perhatian penting terutama dalam mendukung percepatan program Kendaraan Bermotor Listrik Berbasis Baterai (KBLBB). Penelitian ini berfokus pada studi tekno-ekonomi pembangunan pilot plant daur ulang baterai Lithium Manganese Oxide (LMO) dan Lithium Iron Phosphate (LFP) secara pirometalurgi. Kapasitas input daur ulang baterai LFP dan LMO adalah 8.000 ton/tahun. Nilai Internal Rate of Return (IRR), Net Present Value (NPV), Payback Period (PBP), dan Profitability Index (PI) daur ulang baterai LMO berturut-turut adalah 12,57%, Rp.7.583.346.464,-, 5,85 tahun, dan 2,39. Sedangkan untuk daur ulang baterai LFP berturut-turut adalah 11,15%, -Rp.11.235.266.123,-, 6,23 tahun, dan 2,32. Hal ini mengindikasikan daur ulang baterai LMO lebih menjanjikan dibandingkan daur ulang baterai LFP. Dari segi analisis sensitivitas, diketahui bahwa daur ulang baterai LMO dan LFP ini lebih sensitif terhadap perubahan harga produk dibandingkan dengan perubahan harga reagen dan nilai OPEX. Emisi gas CO2, pada proses daur ulang baterai LMO lebih sedikit daripada baterai LFP, sehingga pencemaran lingkungan yang dihasilkan lebih minim. Untuk meminimalisir emisi gas ini, dapat dilakukan instalasi peralatan wet scrubber dan implementasi sistem Carbon Capture and Storage (CCS)/Carbon Capture, Utilization, and Storage (CCUS).
LABINTER: arte e tecnologia em projetos colaborativos
Authors: Oliveira Andréia Machado , Palazuelos Felix Rebolledo , Guerche Tatiana Palma 
Published: May 2023

O artigo aborda alguns projetos colaborativos do grupo de pesquisa LabInter (UFSM), como: REDE_EM_REDE: cartografias artísticas na produção do coletivo, e Documentários Participativos nos Assentamentos do Movimento dos Trabalhadores Rurais Sem Terra. REDE_EM_REDE propõe relações entre as redes sociais online e as redes ferroviárias regionais físicas quase extintas. Com o objetivo de reativar a rede das cidades ferroviárias, incluí na proposta as cidades de Santa Maria, São Gabriel e Santiago no estado de Rio Grande do Sul, Brasil, e tem como principal referencial a Teoria Ator-Rede de Bruno Latour. O segundo projeto, investiga a utilização de dispositivos móveis digitais para produções audiovisuais em rede dentro dos assentamentos do MST/RS, a partir da ideia de ecologia de práticas de Isabelle Stengers.Palavras-chave: LabInter; Projeto colaborativo; Redes sociais; Comunidade; Interatividade. LabInter: Art and Technology in Collaborative ProjectsAbstract: The article discusses some collaborative projects from the research group LabInter (UFSM), such as: REDE_EM_REDE: artistic cartographies in the production of the collective, and Participatory Documentaries in the Settlements of the Landless Rural Workers Movement. REDE_EM_REDE proposes relationships between online social networks and the almost extinct physical regional railroad networks. With the objective of reactivating the network of railway towns, I included in the proposal the cities of Santa Maria, São Gabriel and Santiago in the state of Rio Grande do Sul, Brazil, considering, as the main source of reference, Bruno Latour’s actor-network theory. The second project investigates the mobile digital devices used for networking audiovisual productions within the settlements of the Landless Workers’ Movement (MST), in Rio Grande do Sul, based on Isabelle Stengers' notion of “ecology of practice”. Keywords: LabInter; Collaborative project; Social networks; Community; Interactivity.
Seizures Cause Prolonged Impairment of Ventilation, CO2Chemoreception and Thermoregulation
Authors: Teran Frida A. , Sainju Rup K. , Bravo Eduardo , Wagnon Jacy , Kim YuJaung , Granner Alex , Gehlbach Brian K. , Richerson George B. 
Published: May 2023

Sudden unexpected death in epilepsy (SUDEP) has been linked to respiratory dysfunction, but the mechanisms underlying this association remain unclear. Here we found that both focal and generalized convulsive seizures (GCSs) in epilepsy patients caused a prolonged decrease in the hypercapnic ventilatory response (HCVR; a measure of respiratory CO2chemoreception). We then studiedScn1aR1407X/+(Dravet syndrome; DS) andScn8aN1768D/+(D/+) mice of both sexes, two models of SUDEP, and found that convulsive seizures caused a postictal decrease in ventilation and severely depressed the HCVR in a subset of animals. Those mice with severe postictal depression of the HCVR also exhibited transient postictal hypothermia. A combination of blunted HCVR and abnormal thermoregulation is known to occur with dysfunction of the serotonin (5-hydroxytryptamine; 5-HT) system in mice. Depleting 5-HT withpara-chlorophenylalanine (PCPA) mimicked seizure-induced hypoventilation, partially occluded the postictal decrease in the HCVR, exacerbated hypothermia, and increased postictal mortality in DS mice. Conversely, pretreatment with the 5-HT agonist fenfluramine reduced postictal inhibition of the HCVR and hypothermia. These results are consistent with the previous observation that seizures cause transient impairment of serotonergic neuron function, which would be expected to inhibit the many aspects of respiratory control dependent on 5-HT, including baseline ventilation and the HCVR. These results provide a scientific rationale to investigate the interictal and/or postictal HCVR as noninvasive biomarkers for those at high risk of seizure-induced death, and to prevent SUDEP by enhancing postictal 5-HT tone.SIGNIFICANCE STATEMENTThere is increasing evidence that seizure-induced respiratory dysfunction contributes to the pathophysiology of sudden unexpected death in epilepsy (SUDEP). However, the cellular basis of this dysfunction has not been defined. Here, we show that seizures impair CO2chemoreception in some epilepsy patients. In two mouse models of SUDEP we found that generalized convulsive seizures impaired CO2chemoreception, and induced hypothermia, two effects reported with serotonergic neuron dysfunction. The defects in chemoreception and thermoregulation were exacerbated by chemical depletion of serotonin and reduced with fenfluramine, suggesting that seizure-induced respiratory dysfunction may be due to impairment of serotonin neuron function. These findings suggest that impaired chemoreception because of transient inhibition of serotonergic neurons may contribute to the pathophysiology of SUDEP.
Flow Cytometry of Synaptoneurosomes (FCS) Reveals Increased Ribosomal S6 and Calcineurin Proteins in Activated Medial Prefrontal Cortex to Nucleus Accumbens Synapses
Authors: Rubio F. Javier , Olivares Daniel E. , Dunn Christopher , Zhang Shiliang , Hilaire Elias M. , Henry Akeem , Mejias-Aponte Carlos , Nogueras-Ortiz Carlos J. , Selvam Pooja V. , Cruz Fabio C. , Madangopal Rajtarun , Morales Marisela , Hope Bruce T. 
Published: May 2023

Learning and behavior activate cue-specific patterns of sparsely distributed cells and synapses called ensembles that undergo memory-encoding engram alterations. While Fos is often used to label selectively activated cell bodies and identify neuronal ensembles, there is no comparable endogenous marker to label activated synapses and identify synaptic ensembles. For the purpose of identifying candidate synaptic activity markers, we optimized a flow cytometry of synaptoneurosome (FCS) procedure for assessing protein alterations in activated synapses from male and female rats. After injecting yellow fluorescent protein (YFP)-expressing adeno-associated virus into medial prefrontal cortex (mPFC) to label terminals in nucleus accumbens (NAc) of rats, we injected 20 mg/kg cocaine in a novel context (cocaine+novelty) to activate synapses, and prepared NAc synaptoneurosomes 0–60 min following injections. For FCS, we used commercially available antibodies to label presynaptic and postsynaptic markers synaptophysin and PSD-95 as well as candidate markers of synaptic activity [activity-regulated cytoskeleton protein (Arc), CaMKII and phospho-CaMKII, ribosomal protein S6 (S6) and phospho-S6, and calcineurin and phospho-calcineurin] in YFP-labeled synaptoneurosomes. Cocaine+novelty increased the percentage of S6-positive synaptoneurosomes at 5–60 min and calcineurin-positive synaptoneurosomes at 5–10 min. Electron microscopy verified that S6 and calcineurin levels in synaptoneurosomes were increased 10 min after cocaine+novelty. Pretreatment with the anesthetic chloral hydrate blocked cocaine+novelty-induced S6 and calcineurin increases in synaptoneurosomes, and novel context exposure alone (without cocaine) increased S6, both of which indicate that these increases were due to neural activity per se. Overall, FCS can be used to study protein alterations in activated synapses coming from specifically labeled mPFC projections to NAc.SIGNIFICANCE STATEMENTMemories are formed during learning and are stored in the brain by long-lasting molecular and cellular alterations called engrams formed within specific patterns of cue-activated neurons called neuronal ensembles. While Fos has been used to identify activated ensemble neurons and the engrams within them, we have not had a similar marker for activated synapses that can be used to identify synaptic engrams. Here we developed a procedure for high-throughput in-line analysis of flow cytometry of synaptoneurosome (FCS) and found that ribosomal S6 protein and calcineurin were increased in activated mPFC–NAc synapses. FCS can be used to study protein alterations in activated synapses within specifically labeled circuits.
Progestin-related breast volume changes in a woman with complete androgen insensitivity syndrome (CAIS)
Authors: Dijkman Benthe A M , Blok Christel J M de , Dreijerink Koen M A , Heijer Martin den 
Published: May 2023

Summary
A 31-year-old woman with complete androgen insensitivity syndrome (CAIS) experienced breast volume fluctuations during biphasic hormone replacement therapy consisting of estradiol and cyclical dydrogesterone, a progestin. 3D breast volume measurements showed a 100 cc volume (17%) difference between estradiol monotherapy and combined estradiol and dydrogesterone treatment. Progestogen-dependent breast volume changes have not been reported in the literature. Our findings suggest a correlation between progestogen use and breast volume. Due to the rapid cyclical changes, we hypothesize that the effect is caused by fluid retention.

Learning points

There is limited reports available on the effects of progesterone on breast development and volume.

3D imaging provides an easy-to-use method to quantify breast volume.

The patient in our case description clearly showed that cyclic progesterone use might induce substantial cyclic changes in breast volume.

In women with complete androgen insensitivity syndrome (CAIS), monotherapy with estrogen or continuous supplementation of progesterone might be preferable over cyclic progesterone use.
The Influence of Manager’s Trustworthiness on Subordinate’s Trust in the Manager
Authors: Kameneckienė Vaida 
Published: May 2023

Trust in the organization makes the leadership for the manager easier, contributes to the achievement of the goals. Real and sustainable trust determines the reliability of the entire organization and supports the corporate reputation. A person’s trust in another person eventually leads to the trust of the entire system. Behaviour is a significant determinant of organizational trust. The strongest relationship is between the trustworthiness of the manager and the trust of the subordinate. However, previous studies lack the dimensions of trust. Therefore, in this study, research on the influence of the trustworthiness of the manager on the trust of the subordinate in the manager includes dimensions of each key concept. The purpose of the study is to determine the influence of the trustworthiness of the manager on the subordinate’s trust in the manager. A theoretical model as a background of the research is presented. It is composed of five dimensions of the trustworthiness of the manager, that is, consistency of manager behaviour, integrity of behaviour, sharing of control, and communication and demonstration of concern, and their influence on the general trust, as well as cognitive and emotional trust of subordinates in the manager.
The results of the regression analysis have revealed that the greatest positive influence on the subordinate’s trust in the manager is the manager’s demonstration of concern, but this influence is greater on emotional trust than on cognitive trust. The integrity of the leader’s behaviour also has a positive effect on general and both cognitive and emotional trust of the subordinate in the leader. Additionally, consistency of behaviour, communication, and sharing of control contribute to the accuracy of predicting trust, but the influence of each of these dimensions of the trustworthiness separately is not significant. The results of the study have also shown that the trust of the subordinate increases with the increase in the ratio of the seniority of the subordinate compared to the seniority of the manager. In addition, emotional trust is higher in smaller organizations, while general trust and cognitive trust increase with subordinate age.