bluuebunny/crossref_metadata_2025_split huggingface dataset:
Quantitative proteomics analysis in small cell carcinoma of cervix reveals novel therapeutic targets
Authors: Qiu Haifeng , Su Ning , Wang Jing , Yan Shuping , Li Jing 
Published: April 2023

Abstract
Background
As a rare pathologic subtype, small cell carcinoma of the cervix (SCCC) is characterized by extensive aggressiveness and resistance to current therapies. To date, our knowledge of SCCC origin and progression is limited and sometimes even controversial. Herein, we explored the whole-protein expression profiles in a panel of SCCC cases, aiming to provide more evidence for the precise diagnosis and targeting therapy.

Methods
Eighteen SCCC samples and six matched normal cervix tissues were collected from January 2013 to December 2017. Data independent acquisition mass spectrometry (DIA) was performed to discriminate the different proteins (DEPs) associated with SCCC. The expression of CDN2A and SYP in corresponding SCCC tissues was verified using immunohistochemistry. GO and KEGG enrichment analyses were used to identify the key DEPs related to SCCC development and tumor recurrence.

Results
As a result, 1311 DEPs were identified in SCCC tissues (780 up-regulated and 531 down-regulated). In up-regulated DEPs, both GO analysis and KEGG analysis showed the most enriched were related to DNA replication (including nuclear DNA replication, DNA-dependent DNA replication, and cell cycle DNA replication), indicating the prosperous proliferation in SCCC. As for the down-regulated DEPs, GO analysis showed that the most enriched functions were associated with extracellular matrix collagen-containing extracellular matrix. KEGG analysis revealed that the DEPs were enriched in Complement and coagulation cascades, proteoglycans in cancer, and focal adhesion-related pathways. Down-regulation of these proteins could enhance the mobility of cancer cells and establish a favorable microenvironment for tumor metastasis, which might be accounted for the frequent local and distant metastasis in SCCC. Surprisingly, the blood vessels and circulatory system exhibit a down-regulation in SCCC, which might be partly responsible for its resistance to anti-angiogenic regimens. In the stratification analysis of early-stage tumors, a group of enzymes involved in the cancer metabolism was discriminated in these recurrence cases.

Conclusions
Using quantitative proteomics analysis, we first reported the whole-protein expression profiles in SCCC. Significant alterations were found in proteins associated with the enhancement of DNA replication and cellular motility. Besides the association with mitosis, a unique metabolic feature was detected in cases with tumor recurrence. These findings provided novel targets for disease surveillance and treatments, which warranted further validation in the future.
The impact of bone marrow irradiation dose on acute haematologic toxicity in cervical cancer patients treated with concurrent chemoradiotherapy
Authors: Chen Min , Wang Dajiang , Bao Zhirong , Yi Zongbi , Mei Zijie , Sun Shaoxing , Xiang Qingming , Yang Chunxu , Yang Hui , Qiu Hui , Xie Conghua 
Published: April 2023

Abstract
Objective
To evaluate the impact of bone marrow (BM) irradiation dose on acute haematologic toxicity (HT) in concurrent chemoradiotherapy for cervical cancer.

Methods
Sixty-nine patients with cervical cancer treated with curative or postoperative adjuvant therapy received weekly cisplatin concurrent chemotherapy (CCT) and intensity-modulated radiation therapy (IMRT). The whole pelvic bone marrow (PBM) was delineated and divided into three subsites: ilium (IL), lower pelvis (LP), and lumbosacral spine (LS). Associations between clinical variables, dose volume of BM, including PBM, IL, LP, and LS in the form of x-Vy (volume receiving y Gy for x), and blood cell count nadir were tested using linear regression models. Receiver operating characteristic (ROC) curve analysis was further used to analyse the cutoff values of the variables with p < 0.05 in the multivariate analysis.

Results
In 69 patients, the haemoglobin nadir was positive correlated with baseline haemoglobin (p < 0.001), negative correlated with relative LP-V10 (p = 0.005), relative LP-V25 (p = 0.002), relative LP-V50 (p = 0.007), relative LP-mean (p = 0.003), absolute LP-V15 (p = 0.049), absolute LP-V25 (p = 0.004) and absolute LP-V30 (p = 0.009). The platelet nadir was positive correlated with baseline platelets (p = 0.048) and negative correlated with relative LP-V40 (p = 0.028), but there was no significant variable in absolute radiation volume by multivariate analysis. No variables related to the neutrophil nadir were found, and the 69 patients were divided into group A (43 cases) receiving 3–4 cycles of CCT and group B (26 cases) receiving 5–6 cycles of CCT. In group A, the relative IL-V15 (p = 0.014), the relative IL-V50 (p = 0.010) and the absolute LP-V50 (p = 0.011) were negative correlated with the neutrophil nadir. No significant variable was found in group B. No significant variables related to the lymphocyte nadir were found, and the neutrophil-to-lymphocyte ratio (NLR) was analysed. Age (p < 0.05), relative LP-V15 (p = 0.037) and absolute PBM-mean (p < 0.001) were found to be negative related to NLR.

Conclusion
The dosimetric parameters of relative irradiated volume of BM have more statistically significant datas on acute HT than absolute irradiated volume. The nadir of haemoglobin and platelets and the vertice of NLR were more affected by the irradiation dose to LP, while neutrophils were more affected by the dose to IL. Acute HT was negative related to both low-dose irradiation (V10-30) and high-dose irradiation (V40, V50). For more than 4 cycles of CCT, the effect of BM irradiation on the neutrophils nadir was masked by chemotherapy.
Correlations between 3D preoperative planning and postoperative reduction in the osteosynthesis of distal humeral fractures
Authors: Yoshii Yuichi , Iwabuchi Sho , Ikumi Akira , Kohyama Sho , Ogawa Takeshi , Ishii Tomoo 
Published: April 2023

Abstract
Background
Three-dimensional preoperative planning has been applied to the osteosynthesis of distal humerus fractures. The present study investigated the correlations between 3D preoperative planning and postoperative reduction for the osteosynthesis of distal humerus fractures using 3D parameters.

Methods
Twenty-three elbows of 23 distal humerus fracture patients who underwent osteosynthesis with three-dimensional preoperative planning were evaluated. 3D images of the distal humerus were created after taking preoperative CT scans of the injured elbow. Fracture reduction, implant selection, and placement simulations were performed based on 3D images. Postoperative CT images were taken 1 month after surgery. Correlations were evaluated with preoperative plans and postoperative 3D images. The longitudinal axis and coordinates of the humerus were defined on the 3D images. The coronal angle (CA) was defined as the angle formed by the long axis and the line connecting the medial and lateral margins of the trochlea of the humerus on a coronal plane image. The sagittal angle (SA) was defined as the angle formed by the long axis and the line connecting the top of the lateral epicondyle and the center of the humeral capitellum on a sagittal plane image. The axial angle (AA) was defined as the angle between the sagittal plane and the line connecting the medial and lateral margins behind the trochlea of the humerus. The intraclass correlation coefficients (ICC) of each measurement value were assessed between preoperative planning and postoperative images.

Results
Preoperative planning and postoperative measurement values were CA: 85.6 ± 5.9°/85.8 ± 5.9°, SA: 140.9 ± 8.5°/139.4 ± 7.9°, and AA: 84.0 ± 3.1°/82.6 ± 4.9°, respectively. ICCs were CA: 0.75 (P < 0.01), SA: 0.78 (P < 0.01), and AA: 0.34 (P < 0.05), respectively.

Conclusions
The 3D preoperative planning of distal humeral fractures achieved the good correlations of coronal and sagittal angles, but the relatively poor correlation of the axial angle. This may be attributed to an inability to assess the rotation angle during surgery. We propose the measurement indices shown in the present study as a three-dimensional evaluation index for distal humerus fractures.

Trial registration

Registered as NCT04349319 at ClinicalTrials.gov.
The effects of differing anticoagulant regimes on blood quality after cell salvage in coronary artery bypass grafting (CABG): a pilot study
Authors: Boer Willem , van Tornout Mathias , Brusseleers Maarten , Strauven Maarten , de Vooght Pieter , Vander Laenen Margot , Hoste Eric , Jorens Philippe G. 
Published: April 2023

Abstract
Background
Cell salvage reduces allogenic blood transfusion requirements in surgery. We present a pilot study exploring the impact of anticoagulant choice, citrate or heparin, on the quality of cell salvaged blood in adults undergoing coronary artery bypass grafting (CABG).

Materials and methods
Elective on pump CABG patients were randomly allocated to citrate or heparin anticoagulation. We measured red blood cell characteristics and inflammation in both the blood collection reservoir and the washed red blood cell concentrate. Postoperatively, the level of biomarkers and the coagulation profile in the peripheral blood as well as the transfusion requirements of allogenic blood products were studied.

Results
Thirty eight patients were included, 19 in the citrate group and 19 in the heparin group. Baseline characteristics were similar. In the washed red blood cell concentrate, Mean Hb (g/dl) and Ht (%) were lower in the citrate group [Hb: 18.1 g/dL (SD 1.3) vs. 21.1 (1.6), p < 0.001; Ht: 59.9% (54.7–60.9) vs. 63.7% (62.3–64.8); p < 0.001]; Mean corpuscular volume (MCV, μm 3) was higher [99.1fL (9.4) vs. 88 (4.2), p < 0.001] and mean corpuscular hemoglobin concentration (MCHC, g/dl) lower in the citrate group [31.9 g/dl (29.6–32.4) vs. 33.6 (33.1–34.0) p < 0.001]. Thrombocyte count (1000/μl) was higher in the citrate group [31.0 (26.0–77.0) vs. 13.0 (10.0–39.0); p = 0.006]. There were no differences in the requirement for allogenic blood products’ transfusion (intraoperatively and postoperatively) or in the coagulation parameters after washed red blood cell concentrate infusion. Higher IL-10 was found in the citrate group in the blood collection reservoir, higher neutrophil-derived myeloperoxidase (MPO) in the heparin group after washed red blood cell concentrate infusion.

Conclusion
Though red blood cells in washed red blood cell concentrate were more swollen and diluted in the citrate group with more residual thrombocytes, published quality guidelines were met in both groups. Our pilot study suggests that differences in inflammatory markers in the blood collection reservoir and after infusion of washed red blood cell concentrate indicate a possible pro-inflammatory effect of heparin compared to citrate. A larger study is warranted to confirm these results and their possible clinical consequences.
Trial registrationClinicalTrials.gov: NCT02674906. Registered 5 February 2016.
The increase in cardiac output induced by a decrease in positive end-expiratory pressure reliably detects volume responsiveness: the PEEP-test study
Authors: Lai Christopher , Shi Rui , Beurton Alexandra , Moretto Francesca , Ayed Soufia , Fage Nicolas , Gavelli Francesco , Pavot Arthur , Dres Martin , Teboul Jean-Louis , Monnet Xavier 
Published: April 2023

Abstract
Background
In patients on mechanical ventilation, positive end-expiratory pressure (PEEP) can decrease cardiac output through a decrease in cardiac preload and/or an increase in right ventricular afterload. Increase in central blood volume by fluid administration or passive leg raising (PLR) may reverse these phenomena through an increase in cardiac preload and/or a reopening of closed lung microvessels. We hypothesized that a transient decrease in PEEP (PEEP-test) may be used as a test to detect volume responsiveness.

Methods
Mechanically ventilated patients with PEEP ≥ 10 cmH2O (“high level”) and without spontaneous breathing were prospectively included. Volume responsiveness was assessed by a positive PLR-test, defined as an increase in pulse-contour-derived cardiac index (CI) during PLR ≥ 10%. The PEEP-test consisted in reducing PEEP from the high level to 5 cmH2O for one minute. Pulse-contour-derived CI (PiCCO2) was monitored during PLR and the PEEP-test.

Results
We enrolled 64 patients among whom 31 were volume responsive. The median increase in CI during PLR was 14% (11–16%). The median PEEP at baseline was 12 (10–15) cmH2O and the PEEP-test resulted in a median decrease in PEEP of 7 (5–10) cmH2O, without difference between volume responsive and unresponsive patients. Among volume responsive patients, the PEEP-test induced a significant increase in CI of 16% (12–20%) (from 2.4 ± 0.7 to 2.9 ± 0.9 L/min/m2, p < 0.0001) in comparison with volume unresponsive patients. In volume unresponsive patients, PLR and the PEEP-test increased CI by 2% (1–5%) and 6% (3–8%), respectively. Volume responsiveness was predicted by an increase in CI > 8.6% during the PEEP-test with a sensitivity of 96.8% (95% confidence interval (95%CI): 83.3–99.9%) and a specificity of 84.9% (95%CI 68.1–94.9%). The area under the receiver operating characteristic curve of the PEEP-test for detecting volume responsiveness was 0.94 (95%CI 0.85–0.98) (p < 0.0001 vs. 0.5). Spearman’s correlation coefficient between the changes in CI induced by PLR and the PEEP-test was 0.76 (95%CI 0.63–0.85, p < 0.0001).

Conclusions
A CI increase > 8.6% during a PEEP-test, which consists in reducing PEEP to 5 cmH2O, reliably detects volume responsiveness in mechanically ventilated patients with a PEEP ≥ 10 cmH2O.
Trial registration ClinicalTrial.gov (NCT 04,023,786). Registered July 18, 2019. Ethics Committee approval CPP Est III (N° 2018-A01599-46).
Development of insecticide-impregnated polyester/cotton blend fabric and assessment of their repellent characteristics against Cimex lectularius and dengue vectors Aedes albopictus and Aedes aegypti
Authors: Kakati Ajay , Banerjee Amartya , Das Parikshit , Saha Buddhadeb , Goyary Danswrang , Karmakar Sanjeev , Kishor Sumit , Bhutia Yangchen D. , Chattopadhyay Pronobesh 
Published: April 2023

AbstractBackgroundPersonal protection measures using insecticide-treated fabric is one of the most effective strategies to prevent the bites of hematophagous insects. Many countries have had success treating fabrics with pyrethroids on an individual level.MethodsIn the current study, a new combination of insecticides, alpha-cypermethrin (ACP) and deltamethrin (DET), has been impregnated on fabric composed of a 50:50 blend of polyester and cotton. Residual and morphological analysis was performed along with the evaluation of physical parameters. Biological evaluations were performed to check the repellency, knockdown, and mortality of insecticide-impregnated fabric (IIF) against bed bugs (Cimex lectularius)using Petri plate assay and mosquitoes (Aedes aegyptiandAedes albopictus)using cone bioassay.ResultsThe results showed the repellency of IIF to be 56.6% forC. lectulariusand a knockdown percentage of 53.3% and 63.3% forAe. aegyptiandAe. albopictus, respectively. A > 80% mortality was found for both species of mosquitoes up to 20 cycles of washing with no significant difference (P > 0.05). From high-performance liquid chromatography (HPLC) analysis, the reduction in the contents of ACP and DET after subsequent washes can be correlated with the overall decrease in bioefficacy. ACP and DET remaining in unit gram of fabric after 20 wash cycles were found to be 5.4 mg and 3.1 mg, respectively. By examining the fabric’s surface morphology using scanning electron microscope (SEM) and utilizing energy-dispersive x-ray (EDX) analysis, it was possible to identify the presence of insecticides that were adhered to the fabric. Differential scanning calorimetry (DSC) showed distinctive endothermic peak of insecticide at 98.3 ºC, whereas no change in thermal behavior was observed from thermo-gravimetric analysis (TGA). Furthermore, the physical attributes of IIF provide conclusive evidence for its firmness.ConclusionAll experimental findings were consistent with the potential use of IIF as a bed bug- and mosquito-repellent fabric to be used against hematophagous infestations. This fabric can serve as a potential strategy to control vector-borne diseases like dengue, malaria, trench fever, etc.Graphical Abstract
Effects of foot intensive rehabilitation (FIRE) on clinical outcomes for patients with chronic ankle instability: a randomized controlled trial protocol
Authors: Hoch Matthew C. , Hertel Jay , Gribble Phillip A. , Heebner Nicholas R. , Hoch Johanna M. , Kosik Kyle B. , Long Doug , Sessoms Pinata H. , Silder Amy , Torp Danielle M. , Thompson Katherine L. , Fraser John J. 
Published: April 2023

Abstract
Background
Lateral ankle sprains account for a large proportion of musculoskeletal injuries among civilians and military service members, with up to 40% of patients developing chronic ankle instability (CAI). Although foot function is compromised in patients with CAI, these impairments are not routinely addressed by current standard of care (SOC) rehabilitation protocols, potentially limiting their effectiveness. The purpose of this randomized controlled trial is to determine if a Foot Intensive REhabilitation (FIRE) protocol is more effective compared to SOC rehabilitation for patients with CAI.

Methods
This study will use a three-site, single-blind, randomized controlled trial design with data collected over four data collection points (baseline and post-intervention with 6-, 12-, and 24-month follow-ups) to assess variables related to recurrent injury, sensorimotor function, and self-reported function. A total of 150 CAI patients (50 per site) will be randomly assigned to one of two rehabilitation groups (FIRE or SOC). Rehabilitation will consist of a 6-week intervention composed of supervised and home exercises. Patients assigned to SOC will complete exercises focused on ankle strengthening, balance training, and range of motion, while patients assigned to FIRE will complete a modified SOC program along with additional exercises focused on intrinsic foot muscle activation, dynamic foot stability, and plantar cutaneous stimulation.

Discussion
The overall goal of this trial is to compare the effectiveness of a FIRE program versus a SOC program on near- and long-term functional outcomes in patients with CAI. We hypothesize the FIRE program will reduce the occurrence of future ankle sprains and ankle giving way episodes while creating clinically relevant improvements in sensorimotor function and self-reported disability beyond the SOC program alone. This study will also provide longitudinal outcome findings for both FIRE and SOC for up to two years. Enhancing the current SOC for CAI will improve the ability of rehabilitation to reduce subsequent ankle injuries, diminish CAI-related impairments, and improve patient-oriented measures of health, which are critical for the immediate and long-term health of civilians and service members with this condition.
Trial Registration Clinicaltrials.gov Registry: NCT #NCT04493645 (7/29/20).
Relations in the context of Turiyam sets
Authors: Ganati Gamachu Adugna , Srinivasa Rao Repalle V. N. , Ashebo Mamo Abebe 
Published: April 2023

Abstract
Objective
Recently, the Turiyam set was introduced as an extension of the neutrosophic set to handle the uncertainty data set beyond its truth, indeterminacy and falsity values. This article introduced the Cartesian product of Turiyam sets and Turiyam relations. Further, we defined operations on Turiyam relations as well as discussed the inverse and types of Turiyam relations.

Results
The Cartesian product of Turiyam sets, Turiyam relations, inverse Turiyam relation and types of Turiyam relations are stated and their properties are derived. Furthermore, examples are given to clarify some concepts.
Empirical retinal venous pulse wave velocity using modified photoplethysmography
Authors: Abdul-Rahman Anmar , Morgan William , Vukmirovic Aleksandar , Mehnert Andrew , Obreschow Danail , Yu Dao-Yi 
Published: April 2023

Abstract
Objective
Using the novel imaging method of high-speed modified photoplethysmography we measured the retinal venous pulse wave velocity in a single case.

Results
A healthy 30-year-old subject underwent high-speed modified photoplethysmography (120 frames per second) with simultaneous ophthalmodynamometry at 26 Meditron units. A video of the optic nerve was analyzed using custom software. A harmonic regression model was fitted to each pixel in the time series and used to quantify the retinal vascular pulse wave parameters. Retinal venous pulsation at the optic disc was observed as a complex dynamic wall motion, whereas contraction commenced at a point in the vein at the center of the optic disc, and progressed centrifugally. The empirically estimated retinal venous pulse wave velocity at this segment was approximately 22.24694 mm/s. This measurement provides an estimate for future studies in the field.
MolFilterGAN: a progressively augmented generative adversarial network for triaging AI-designed molecules
Authors: Liu Xiaohong , Zhang Wei , Tong Xiaochu , Zhong Feisheng , Li Zhaojun , Xiong Zhaoping , Xiong Jiacheng , Wu Xiaolong , Fu Zunyun , Tan Xiaoqin , Liu Zhiguo , Zhang Sulin , Jiang Hualiang , Li Xutong , Zheng Mingyue 
Published: April 2023

AbstractArtificial intelligence (AI)-based molecular design methods, especially deep generative models for generating novel molecule structures, have gratified our imagination to explore unknown chemical space without relying on brute-force exploration. However, whether designed by AI or human experts, the molecules need to be accessibly synthesized and biologically evaluated, and the trial-and-error process remains a resources-intensive endeavor. Therefore, AI-based drug design methods face a major challenge of how to prioritize the molecular structures with potential for subsequent drug development. This study indicates that common filtering approaches based on traditional screening metrics fail to differentiate AI-designed molecules. To address this issue, we propose a novel molecular filtering method, MolFilterGAN, based on a progressively augmented generative adversarial network. Comparative analysis shows that MolFilterGAN outperforms conventional screening approaches based on drug-likeness or synthetic ability metrics. Retrospective analysis of AI-designed discoidin domain receptor 1 (DDR1) inhibitors shows that MolFilterGAN significantly increases the efficiency of molecular triaging. Further evaluation of MolFilterGAN on eight external ligand sets suggests that MolFilterGAN is useful in triaging or enriching bioactive compounds across a wide range of target types. These results highlighted the importance of MolFilterGAN in evaluating molecules integrally and further accelerating molecular discovery especially combined with advanced AI generative models.