bluuebunny/crossref_metadata_2025_split huggingface dataset:
Impact of preoperative docetaxel, cisplatin, and 5-fluorouracil (DCF) therapy on degree of malignant esophageal stenosis
Authors: Yamaguchi Takahisa , Okamoto Koichi , Saito Hiroto , Shimada Mari , Tsuji Toshikatsu , Moriyama Hideki , Kinoshita Jun , Nakamura Keishi , Inaki Noriyuki 
Published: August 2023

Abstract
Background
Malignant esophageal stenosis is a common and severe complication of advanced esophageal cancer that can be a serious problem in the continuation of chemotherapy and other anticancer treatments. The impact of chemotherapy regimens on the degree of improvement in esophageal stenosis is unknown. In this study, we focused on the impacts of chemotherapy on the direct anticancer effects, and in the improvement of malignant stenosis.

Methods
Patients who underwent radical esophagectomy after chemotherapy, either adjuvant 5-fluorouracil and cisplatin (FP) or docetaxel, cisplatin, and 5-fluorouracil (DCF) regimen, were included. We assessed the length of the cancerous stenosis, the width of the narrowest segment, and the size of the intraluminal area in the stenotic segment by fluoroscopy, and compared the differences before and after chemotherapy. In addition, we evaluated the dysphagia score (Mellow-Pinkas scoring system) as the evaluation of patients’ symptoms. The antitumor effects of chemotherapy were also investigated.

Results
A total of 81 patients were enrolled: 50 were treated with FP, and 31 were treated with DCF. The expansion rate in the length of the narrowest part was significantly increased in the DCF group compared with the FP group. Furthermore, the stenosis index (intraluminal stenotic area/stenotic length) was significantly increased in the DCF group compared with the FP group (112% vs 96%, P = 0.038). Dysphagia score after chemotherapy significantly improved in the DCF group compared to the FP group (P = 0.007). The response rates were 60% in the FP group and 67.7% in the DCF group. Effective histopathological response (improvement to grade 2 or 3) was 24% in the FP group and 38.8% in the DCF group.

Conclusion
DCF therapy is more effective than FP treatment in the improvement of malignant esophageal stenosis.
Factors associated with diagnostic delay of pulmonary tuberculosis among children and adolescents in Quzhou, China: results from the surveillance data 2011–2021
Authors: Zhang Yating , Zhan Bingdong , Hao Xiaogang , Wang Wei , Zhang Xing , Fang Chunfu , Wang Min 
Published: August 2023

Abstract
Purpose
Tuberculosis is a high-burden disease and a major health concern in China, especially among children and adolescents. The purpose of this study was to assess risk factors for diagnostic delay in students with pulmonary tuberculosis in Quzhou City in eastern China.

Patients and methods
Cases of PTB in students and relevant information in Quzhou from 2011 to 2021 were collected using the TB Management Information System. The outcome of interest was diagnostic delay (i.e. ≥ 28 days between symptom onset and treatment initiation). Risk factors for diagnostic delay were identified using multivariable logistic regression.

Results
A total of 629 students in Quzhou were diagnosed with PTB during the study period, of whom 55.5% were male. The median diagnostic delay was 18 days (Inter Quartile Range, [IQR]: 8–38) and 38.0% of the students had a diagnostic delay. Living in a rural area (adjusted odds ratio, [AOR]: 1.56, 95% confidence interval [CI:] 1.11–2.19), developing PTB symptoms in the first quarter of the year (AOR: 2.18, 95% CI: 1.40–3.40), and no sputum smear result (AOR: 8.73, 95% CI: 1.68–45.30) were significantly associated with a diagnostic delay. Discovery through health examinations (AOR: 0.33, 95% CI: 0.17–0.63) was associated with reduced risk of diagnostic delay.

Conclusion
Schools in rural areas should pay special attention to increasing student awareness of the symptoms of tuberculosis and provide health education on tuberculosis prevention and control to students and staff.
Tubular basement membrane deposits after allogeneic hematopoietic stem cell transplantation
Authors: Zhou Wenyan , Qi Chaojun , Zhang Minfang , Hou Xiaotao , Ni Zhaohui 
Published: August 2023

Abstract
Background
Extraglomerular immune complex deposition is rare and only a few membranous nephropathy cases with tubular basement membrane deposits have been reported following allogeneic hematopoietic stem cell transplantation.

Case presentation
We reported a 56-year-old man with increased serum creatinine after allogeneic hematopoietic stem cell transplantation who underwent a renal biopsy. Tubular interstitial nephritis was identified on light microscope. The unique histologic features were diffuse tubular basement membrane immune complex deposition detected by both immunofluorescence and electron microscopy, while the glomerular involvement was inconspicuous. The differential diagnosis from other forms of tubular basement membrane deposition is discussed.

Conclusion
Diffuse granular tubular basement membrane immune complex deposition with minimal glomerular involvement is also a manifestation of renal complication in hematopoietic stem cell transplantation recipient. However, the exact mechanism and target antigen remains unknown.
COVCOG: Immediate and long-term cognitive improvement after cognitive versus emotion management psychoeducation programs - a randomized trial in covid patients with neuropsychological difficulties
Authors: Willems Sylvie , Didone Vincent , Cabello Fernandez Carmen , Delrue Gael , Slama Hichem , Fery Patrick , Goin Julien , Della Libera Clara ,   COVCOG Group, Moutschen Michel , Rousseau Anne-Françoise , Dupuis Gilles , Billet Maud , Charonitis Maëlle , Demoulin Valentine , Dethier Marie , Guillemin Camille , Kreusch Fanny , Leens Fréderique , Léonard Christina , Lesoinne Alexia , Requier Florence , Reyt Mathilde , Rotsaert Marianne , Collette Fabienne 
Published: August 2023

Abstract
Background
Cognitive difficulties are a frequent complaint in long COVID and persist for more than a year post- infection. There is a lack of evidence-based data on effective intervention strategies. Non-pharmacological intervention programs that are used with other neurological populations have not yet been the subject of controlled trials. COVCOG is a multicentric, randomized trial comparing cognitive intervention and a cognitive-behavioural counselling.

Methods/design
Patients with long covid are selected and recruited at least three months post-infection. Patients are randomised in a 1:1 ratio into the cognitive (neuropsychological psychoeducation) and affective (emotion management with cognitive-behavioural counselling) intervention arms. The inclusion of 130 patients is planned. The cognitive intervention includes psycho-educational modules on fatigue and sleep, attention and working memory, executive functions and long-term memory. The affective intervention includes modules on emotion recognition and communication, uncertainty management and behavioral activation. The main objective is to reduce cognitive complaints 2 months after the intervention. A Follow-up is also planned at 8 months.

Discussion
Given the long-term effects of Covid on cognition and the negative effects of cognitive impairment on quality of life and social participation, it is important to determine whether low-dose, non-pharmacological interventions can be effective. The trial will determine which of the usual types of intervention is the most effective.

Trial registration
Clinicaltrials.gov Number: NCT05167266 (21/12/ 2021).
A novel super-enhancer-related gene signature predicts prognosis and immune microenvironment for breast cancer
Authors: Wu Qing , Tao Xuan , Luo Yang , Zheng Shiyao , Lin Nan , Xie Xianhe 
Published: August 2023

Abstract
Background
This study targeted at developing a robust, prognostic signature based on super-enhancer-related genes (SERGs) to reveal survival prognosis and immune microenvironment of breast cancer.

Methods
RNA-sequencing data of breast cancer were retrieved from The Cancer Genome Atlas (TCGA), 1069 patients of which were randomly assigned into training or testing set in 1:1 ratio. SERGs were downloaded from Super-Enhancer Database (SEdb). After which, a SERGs signature was established based on the training set, with its prognostic value further validated in the testing set. Subsequently, we identified the potential function enrichment and tumor immune infiltration of the model. Moreover, in vitro experiments were completed to further explore the biological functions of ZIC2 gene (one of the risk genes in the prognostic model) in breast cancer.

Results
A risk score system of prognostic value was constructed with 6 SERGs (ZIC2, NFE2, FOXJ1, KLF15, POU3F2 and SPIB) to find patients in high-risk group with significantly worse prognosis in both training and testing sets. In addition, a multivariate regression was established via integrating the 6 genes with age and N stage, indicating well performance by calibration, time-dependent receiver operating characteristic (ROC) analysis and decision curve analysis (DCA). Further analysis demonstrated that tumor-associated pathological processes and pathways were significantly enriched in the high-risk group. In general, the novel SERGs signature could be applied to screen breast cancer with immunosuppressive microenvironment for the risk score was negatively correlated with ESTIMATE score, tumor-infiltration lymphocytes (such as CD4 + and CD8 + T cell), immune checkpoints and chemotactic factors. Furthermore, down-regulation of ZIC2 gene expression inhibited the cell viability, cellular migration and cell cycle of breast cancer cells.

Conclusions
The novel SERGs signature could predict the prognosis of breast cancer; and SERGs might serve as potential therapeutic targets for breast cancer.
Accuracy and reproducibility analysis of different reference axes for femoral prosthesis rotation alignment in TKA based on 3D CT femoral model
Authors: Liu Kun , Liu Xuande , Guan Yujun , Ma Haotong , Fu Donglin , Fan Zongqing 
Published: August 2023

Abstract
Background
There are many reference axes to determine the rotational positioning of the femoral prosthesis in total knee arthroplasty (TKA), mainly including the surgical transepicondylar axis (sTEA), anatomical transepicondylar axis (aTEA), Whiteside line, and the posterior condylar line (PCL), etc., but there is still no definite conclusion on which is the most accurate reference axis.

Objective
To explore the reproducibility of each reference axis of femoral external osteotomy based on the 3D CT femoral model, compare the deviation of the simulated femoral prosthesis rotation alignment, positioned based on each reference axis, with the gold standard sTEA, and analyze the accuracy of each reference axis.

Methods
The imaging data of 120 patients with knee osteoarthritis who underwent a 3D CT examination of the knee in our hospital from June 2018 to December 2021 were retrospectively collected. The 3D model of the femur was established by Mimics software. The line relative to PCL externally rotated 3° (PCL + 3°), aTEA, and the vertical line of the Whiteside line were constructed and compared with the gold standard sTEA. Intra-observer, as well as inter-observer reproducibility analysis, was performed by the intra-group correlation coefficient (ICC) and Bland-Altman method.

Results
The angle ∠WS, between the vertical line of Whiteside and sTEA, was 2.54 ± 2.30°, with an outlier of 54.2%; the angle ∠aTEA, between aTEA and sTEA, was 4.21 ± 1.01°, with an outlier of 99.1%; the angle ∠PCL, between PCL + 3° external rotation and sTEA, was 0.50 ± 1.06°, with the highest accuracy and an outlier of 5.8%, and the differences among all three were statistically significant, P < 0.05. The intra-observer ICC values of ∠WS, ∠aTEA, and ∠PCL were 0.975 (0.964–0.982), 0.926 (0.896–0.948), and 0.924(0.892,0.946), respectively, and the reproducibility levels were excellent; the inter-observer ICC values of ∠WS, ∠aTEA, and ∠PCL were 0.968(0.955–0.978), 0.906 (0.868–0.934) and 0.970 (0.957,0.979), respectively, with excellent reproducibility levels; Bland-Altman plots suggested that the scatter points of intra-observer and inter-observer measurement differences more than 95% were within the limits of agreement.

Conclusion
The reference axis for locating the distal femoral external rotation osteotomy based on the 3D CT femoral model has good reproducibility. The PCL is easy to operate, has the highest precision, and the lowest outliers among the reference axes is therefore recommended.
Determination of serum CA724 levels using fluorescence immunochromatography
Authors: Liu Chang , Chen Cuicui , Peng Fenglan , Liang Huankun , Zhong Shuhai , Liu Tiancai , Li Laiqing , Boronin Alexander , Dong Wenqi 
Published: August 2023

Abstract
Background
Carbohydrate antigen 724 (CA724) is a sensitive and specific indicator for multiple malignant tumors. The aim of this study was to establish a Eu-time resolved fluorescence immunochromatography (Eu-TRFICO) method for quantitative detection of CA724 in serum.

Methods
Eu-TRFICO strips were optimized and assembled. The sensitivity, specificity and precision were evaluated using CA724 standard dilutions and matrix serum. Meanwhile, the reference interval, comparison, and sensitivity/specificity were performed using clinical negative/positive gastric cancer serum samples.

Results
The standard curve equation was y = 9.869 x − 154.12 (R2 = 0.993), and the sensitivity was 0.42 U/mL. The common interferents in serum could not affect the quantitative results with low cross-reactivities (all no more than 1.09%). All average recoveries of the intra- and interbatch ranged from 102.38 to 106.40%, and all CVs were below 10%. The reference interval of the healthy subjects was < 4.68 U/mL and the reference interval of the subjects with grade I/II gastric cancer was > 9.54 U/mL. Additionally, a high Pearson r (0.9503) and sensitivity/specificity (92.86%/94.20%) were obtained.

Conclusion
This study prepared Eu-TRFICO strips with high sensitivity, specificity, precision and satisfactory clinical testing performance, which provides more options for clinical quantitative and convenient testing of CA724.
Readiness for interprofessional learning among health science students: a cross-sectional Q-methodology and likert-based study
Authors: Oliveira Ana , Brewer-Deluce Danielle , Akhtar-Danesh Noori , Wojkowski Sarah 
Published: August 2023

Abstract
Background
Interprofessional education (IPE) prepares healthcare students for collaboration in clinical practice, but the effectiveness of this teaching method depends on students’ readiness for and perceptions of IPE. Evaluating students’ readiness for and perceptions of IPE is challenging, due to the lack of comprehensive measures. This study characterized the level of IPE readiness and perspectives across first-year undergraduate and graduate health science students using the readiness for interprofessional learning Likert Scale (RIPLS) and Q-methodologies.

Methods
This is a cross-sectional, online study. Students were randomized to answer the Likert-scale version of RIPLS (80%) or a matched Q-methodology survey (20%). An ANCOVA compared RIPLS scores between students from different program levels (graduate/undergraduate) and specialization (health professional and general programs). The Q-data was analysed using a by-person factor analysis.

Results
Three hundred and four (33% response rate) and 71 (30% response rate) students completed the Likert scale and the Q-methodology surveys, respectively. Students from graduate programs demonstrated high readiness for IPE (higher total RIPLS scores p < 0.001) in comparison to undergraduates. Three factors, associated with program specialization (p = 0.04), emerged from the Q-methodology analysis characterizing students learning priorities. Students in undergraduate general programs were focused on IPE relevance and benefits to “the clinical team”, students in graduate programs focused on “the patient”, and those in undergraduate health professional programs focused on themselves (“me”).

Conclusions
This novel mixed-methods approach combining traditional Likert-scales with Q-methodology elucidated not only associations between program and specialization with readiness (Likert) but also which components of IPE were valued the most (Q-methodology) and by whom.
Small area analysis methods in an area of limited mapping: exploratory geospatial analysis of firearm injuries in Port-au-Prince, Haiti
Authors: Burlotos Athanasios , Pierre Tayana Jean , Johnson Walter , Wiafe Seth ,   PROTRA Haiti Group, Pean Corinne , McCalla Monet , Lominy Carl Stephane , Lefevre Jean Antoni Sebastien , Lot Yvelie Saint , Lartigue Jean Wilguens , Therone Alence , Torres Carolina , Cahill Gabrielle , Joseph Michelle 
Published: August 2023

AbstractBackgroundThe city of Port-au-Prince, Haiti, is experiencing an epidemic of firearm injuries which has resulted in high burdens of morbidity and mortality. Despite this, little scientific literature exists on the topic. Geospatial research could inform stakeholders and aid in the response to the current firearm injury epidemic. However, traditional small-area geospatial methods are difficult to implement in Port-au-Prince, as the area has limited mapping penetration. Objectives of this study were to evaluate the feasibility of geospatial analysis in Port-au-Prince, to seek to understand specific limitations to geospatial research in this context, and to explore the geospatial epidemiology of firearm injuries in patients presenting to the largest public hospital in Port-au-Prince.ResultsTo overcome limited mapping penetration, multiple data sources were combined. Boundaries of informally developed neighborhoods were estimated from the crowd-sourced platform OpenStreetMap using Thiessen polygons. Population counts were obtained from previously published satellite-derived estimates and aggregated to the neighborhood level. Cases of firearm injuries presenting to the largest public hospital in Port-au-Prince from November 22nd, 2019, through December 31st, 2020, were geocoded and aggregated to the neighborhood level. Cluster analysis was performed using Global Moran’s I testing, local Moran’s I testing, and the SaTScan software. Results demonstrated significant geospatial autocorrelation in the risk of firearm injury within the city. Cluster analysis identified areas of the city with the highest burden of firearm injuries.ConclusionsBy utilizing novel methodology in neighborhood estimation and combining multiple data sources, geospatial research was able to be conducted in Port-au-Prince. Geospatial clusters of firearm injuries were identified, and neighborhood level relative-risk estimates were obtained. While access to neighborhoods experiencing the largest burden of firearm injuries remains restricted, these geospatial methods could continue to inform stakeholder response to the growing burden of firearm injuries in Port-au-Prince.
Testing the influence of harm reduction messages on health risk attitudes, injunctive norms and perceived behavioral control
Authors: Katz Sherri Jean , Cohen Elisia , Hatsukami Dorothy 
Published: August 2023

Abstract
Background
E-cigarettes can potentially be a harm reduction pathway for adults who smoke and who are seeking to make the complete switch from cigarettes. However, often people who smoke believe that e-cigarettes are just as damaging as cigarettes to their health. From a harm reduction perspective, the key question is whether providing information about the reduced toxicant intake of e-cigarettes, compared to cigarettes, could influence their perceptions and whether there are certain message features that might further support this transition.

Methods
In this experiment (n = 305), we test whether a harm reduction (reduced toxicant intake, complete switch) message will influence the health risk attitudes, injunctive norms and perceived behavioral control of people who smoke, compared to those who do not view a message and whether including a “smoking cue” within the message influences their response.

Results
Results indicate that those who viewed the harm reduction message with a smoking cue had lower health risk attitudes than those who did not view a message (p = 0.025) and higher injunctive norms than those who viewed the message without a smoking cue (p = 0.006).

Conclusions
These findings demonstrate that a harm reduction message with a smoking cue can influence the perceptions of adults who smoke, lowering health risk attitudes and increasing injunctive norms.
A novel inductively coupled capacitor wireless sensor system for rapid antibiotic susceptibility testing
Authors: Xu Yikang , Ren Dacheng 
Published: August 2023

Abstract
Background
The increasing prevalence and severity of antimicrobial resistance (AMR) present a major challenge to our healthcare system. Rapid detection of AMR is essential for lifesaving under emergent conditions such as sepsis. The current gold standard phenotypic antibiotic susceptibility testing (AST) takes more than a day to obtain results. Genotypic ASTs are faster (hours) in detecting the presence of resistance genes but require specific probes/knowledge of each AMR gene and do not provide specific information at the phenotype level. To address this unmet challenge, we developed a new rapid phenotypic AST.

Result
We designed a new electrochemical biosensor based on the concept of magnetically coupled LC sensors. The engineered LC sensors can be placed in 96-well plates and communicate the reading remotely with a receiver coil for signal analysis. The sensors were validated by monitoring the growth of Escherichia coli, Staphylococcus aureus, and Pseudomonas aeruginosa in the presence and absence of different antibiotics. Drug-resistant strains were used as controls. Bacterial growth was detected within 30 min after inoculation, allowing rapid determination of antibiotic susceptibility at the phenotype level. The sensor also functions in the presence of host proteins when tested with 2% FBS in growth media.

Conclusions
With the compatibility with 96-well plates, this label-free rapid 30-min AST has the potential for low-cost applications with simple integration into the existing workflow in clinical settings.
Challenges and new technologies in adoptive cell therapy
Authors: Zhang Pengchao , Zhang Guizhong , Wan Xiaochun 
Published: August 2023

AbstractAdoptive cell therapies (ACTs) have existed for decades. From the initial infusion of tumor-infiltrating lymphocytes to the subsequent specific enhanced T cell receptor (TCR)-T and chimeric antigen receptor (CAR)-T cell therapies, many novel strategies for cancer treatment have been developed. Owing to its promising outcomes, CAR-T cell therapy has revolutionized the field of ACTs, particularly for hematologic malignancies. Despite these advances, CAR-T cell therapy still has limitations in both autologous and allogeneic settings, including practicality and toxicity issues. To overcome these challenges, researchers have focused on the application of CAR engineering technology to other types of immune cell engineering. Consequently, several new cell therapies based on CAR technology have been developed, including CAR-NK, CAR-macrophage, CAR-γδT, and CAR-NKT. In this review, we describe the development, advantages, and possible challenges of the aforementioned ACTs and discuss current strategies aimed at maximizing the therapeutic potential of ACTs. We also provide an overview of the various gene transduction strategies employed in immunotherapy given their importance in immune cell engineering. Furthermore, we discuss the possibility that strategies capable of creating a positive feedback immune circuit, as healthy immune systems do, could address the flaw of a single type of ACT, and thus serve as key players in future cancer immunotherapy.