bluuebunny/crossref_metadata_2025_split huggingface dataset:
The kinematic structure of magnetically aligned H i filaments
Authors: Kim Doyeon A , Clark S E , Putman M E , Li Larry 
Published: September 2023

ABSTRACT
We characterize the kinematic and magnetic properties of H i filaments located in a high Galactic latitude region (165° &lt; α &lt; 195° and 12° &lt; δ &lt; 24°). We extract three-dimensional filamentary structures using fil3d from the Galactic Arecibo L-Band Feed Array H i (GALFA-H i) survey 21-cm emission data. Our algorithm identifies coherent emission structures in neighbouring velocity channels. Based on the mean velocity, we identify a population of local and intermediate velocity cloud (IVC) filaments. We find the orientations of the local (but not the IVC) H i filaments are aligned with the magnetic field orientations inferred from Planck 353 GHz polarized dust emission. We analyse position–velocity diagrams of the velocity-coherent filaments, and find that only 15 per cent of filaments demonstrate significant major-axis velocity gradients with a median magnitude of 0.5 km s−1 pc−1, assuming a fiducial filament distance of 100 pc. We conclude that the typical diffuse H i filament does not exhibit a simple velocity gradient. The reported filament properties constrain future theoretical models of filament formation.
A global turbulence model shows excellent atmospheric optical turbulence conditions over the Ethiopian plateau during non-rainy season
Authors: Qing Chun , Bi Cuicui , Zhu Wenyue , Qian Xianmei , Kang Li , Li Xuebin , Wu Xiaoqing 
Published: September 2023

ABSTRACT
A worldwide search is currently being conducted to determine the most appropriate sites for the next generation of large optical and infrared telescopes. Here, we report a global map of atmospheric optical turbulence-associated parameter (atmospheric coherence length) based on the fifth generation of European Centre for Medium Range Weather Forecasts re-analysis data set and results for atmospheric coherence lengths at European Southern Observatory and Tibetan Plateau locations (all at mid-latitudes). These confirm the accuracy of the global atmospheric optical turbulence model, but also the simulation result reveals that an excellent atmospheric calm site exist at the Ethiopian Plateau (at a latitude of ∼10°N) outside of the rainy season. The Ethiopian Plateau is the highest plateau in Africa, known as the ‘Roof of Africa’, with an average elevation of over 2500 m, and thus potentially provides good opportunities for astronomy and astrophysics. The median atmospheric coherence length at the Ethiopian Plateau was 17.7 cm and approximately 75 per cent of the time above 15.6 cm for dry conditions, indicating a very calm atmosphere. Although favourable atmospheric turbulence conditions at the Ethiopian Plateau were found from the global turbulence model, due to the complete lack of infrastructure it has never been visited, meaning that additional validation experiments are required.
Chemical enrichment of ICM within the Ophiuchus cluster I: radial profiles
Authors: Gatuzz Efrain , Sanders J S , Dennerl K , Liu A , Fabian A C , Pinto C , Eckert D , Walker S A , ZuHone J 
Published: September 2023

ABSTRACT
The analysis of the elemental abundances in galaxy clusters offers valuable insights into the formation and evolution of galaxies. In this study, we explore the chemical enrichment of the intracluster medium in the Ophiuchus cluster by utilizing XMM–Newton EPIC-pn observations. We explore the radial profiles of Si, S, Ar, Ca, and Fe. Due to the high absorption of the system, we have obtained only upper limits for O, Ne, Mg, and Ni. We model the X/Fe ratio profiles with a linear combination of core-collapse supernovae (SNcc) and Type Ia supernovae (SNIa) models. We found a flat radial distribution of SNIa ratio over the total cluster enrichment $10\!-\!30{{\ \rm per\ cent}}$ for all radii. However, the absence of light α-elements abundances may lead to overestimation of the SNcc contribution.
Asteroseismology of double-mode radial δ Scuti stars: AE Ursae Majoris and RV Arietis
Authors: Daszyńska-Daszkiewicz J , Walczak P , Szewczuk W , Niewiadomski W 
Published: September 2023

ABSTRACT
We construct complex seismic models of two high-amplitude δ Sct stars, AE UMa and RV Ari, each pulsating in two radial modes: fundamental and first overtone. Besides the frequencies of two radial modes, the models also reproduce the amplitude of bolometric flux variations (the non-adiabatic parameter f) for the dominant mode. Applying the Monte-Carlo-based Bayesian analysis, we derive strong constraints on the parameters of the model, as well as on the free parameters of the theory. A vast majority of seismic models of the two stars are just at the beginning of hydrogen-shell burning and a small fraction are at the very end of an overall contraction. The stars have a similar age of about 1.6 Gyr for the hydrogen-shell burning phase. Both stars have unusual low overshooting from the convective core, about 0.02 and 0.004 of the pressure scaleheight for AE UMa and RV Ari, respectively. This result presumably indicates that overshooting should vary with time and scale with a decreasing convective core. The efficiency of convection in the envelope of both stars is low and is described by the mixing length parameter αMLT of about 0.3–0.6. The third frequency of RV Ari, confirmed by us in the Transiting Exoplanet Survey Satellite (TESS) photometry, can only be associated with mixed non-radial modes ℓ = 1, g4–g8 or ℓ = 2, g10–g12. We include the dipole mode into our Bayesian modelling and demonstrate its huge asteroseismic potential.
Stellar bars in spinning haloes: delayed buckling and absence of slowdown
Authors: Li Xingchen , Shlosman Isaac , Heller Clayton , Pfenniger Daniel 
Published: September 2023

ABSTRACT
We use numerical simulations to analyse the stellar bar evolution in spinning dark matter (DM) haloes. Previous works have shown the halo spin has a substantial effect on bar evolution and can lead to bar dissolution following the vertical buckling instability. We invoke the DM spin sequence, λ = 0–0.09, and study the effect of DM density along this λ sequence by varying compactness of DM halo. We find that (1) varying DM density has a profound effect on bar evolution along λ sequence. (2) For λ ≳ 0.045, the buckling has been delayed progressively. (3) Stellar bars remain near maximal strength, and their amplitude plateau stage extends over 0.7–5 Gyr, terminating with the buckling. (4) Although stellar bars remain strong during the plateau, their pattern speed and size stay nearly constant. This unusual behaviour of stellar bars follows from highly reduced gravitational torques due to DM bar being aligned with the stellar bar. The orbital analysis shows that delayed buckling results from slow evolution of stellar oscillations along bar major and vertical axes, thus postponing the action of the vertical 2:1 resonance which pumps the rotational energy into vertical motions. (5) Peanut/boxy-shaped bulges form at the beginning of the plateau and grow with time. (6) Finally, strong bars in spinning haloes can avoid fast braking, resolving the long-standing discrepancy between observations and N-body simulations. This behaviour of stellar bars along the λ and DM density sequences reveals a wealth of stellar bar properties which require additional study.
Genetic Origins and Adaptive Evolution of the Deng People on the Tibetan Plateau
Authors: Ge Xueling , Lu Yan , Chen Shuanghui , Gao Yang , Ma Lifeng , Liu Lijun , Liu Jiaojiao , Ma Xixian , Kang Longli , Xu Shuhua 
Published: September 2023

Abstract
The Tibetan Plateau is populated by diverse ethnic groups, but most of them are underrepresented in genomics studies compared with the Tibetans (TIB). Here, to gain further insight into the genetic diversity and evolutionary history of the people living in the Tibetan Plateau, we sequenced 54 whole genomes of the Deng people with high coverage (30–60×) and analyzed the data together with that of TIB and Sherpas, as well as 968 ancient Asian genomes and available archaic and modern human data. We identified 17.74 million novel single-nucleotide variants from the newly sequenced genomes, although the Deng people showed reduced genomic diversity and a relatively small effective population size. Compared with the other Tibetan highlander groups which are highly admixed, the Deng people are dominated by a sole ancestry that could be traced to some ancient northern East Asian populations. The divergence between Deng and Tibetan people (∼4,700–7,200 years) was more recent than that between highlanders and the Han Chinese (Deng-HAN, ∼9,000–14,000 years; TIB-HAN, 7,200–10,000 years). Adaptive genetic variants (AGVs) identified in the Deng are only partially shared with those previously reported in the TIB like HLA-DQB1, whereas others like KLHL12 were not reported in TIB. In contrast, the top candidate genes harboring AGVs as previously identified in TIB, like EPAS1 and EGLN1, do not show strong positive selection signals in Deng. Interestingly, Deng also showed a different archaic introgression scenario from that observed in the TIB. Our results suggest that convergent adaptation might be prevalent on the Tibetan Plateau.
Preclinical pediatric brain tumor models for immunotherapy: Hurdles and a way forward
Authors: Mishra Deepak Kumar , Popovski Dean , Morris Shelli M , Bondoc Andrew , Senthil Kumar Shiva , Girard Emily J , Rutka James , Fouladi Maryam , Huang Annie , Olson James M , Drissi Rachid 
Published: September 2023

AbstractBrain tumors are the most common solid tumor in children and the leading cause of cancer-related deaths. Over the last few years, improvements have been made in the diagnosis and treatment of children with Central Nervous System tumors. Unfortunately, for many patients with high-grade tumors, the overall prognosis remains poor. Lower survival rates are partly attributed to the lack of efficacious therapies. The advent and success of immune checkpoint inhibitors (ICIs) in adults have sparked interest in investigating the utility of these therapies alone or in combination with other drug treatments in pediatric patients. However, to achieve improved clinical outcomes, the establishment and selection of relevant and robust preclinical pediatric high-grade brain tumor models is imperative. Here, we review the information that influenced our model selection as we embarked on an international collaborative study to test ICIs in combination with epigenetic modifying agents to enhance adaptive immunity to treat pediatric brain tumors. We also share challenges that we faced and potential solutions.
Hosting the Tobacco Industry Supply Chain and Political Interference
Authors: Hiscock Rosemary , Alaouie Hala , Matthes Britta K , Mehegan John , Bloomfield Michael J 
Published: September 2023

Abstract

Introduction
We found no comprehensive studies on the location of transnational tobacco and leaf company (TTLC) subsidiaries (business entities they control) or the consequences of their presence on health policy.

Aims and Methods
Here we assess (1) the global reach of TTLCs by mapping their tobacco growing and manufacturing subsidiaries and (2) the relationship between in-country presence of the tobacco industry and their power and interference. Data on subsidiaries were collated through systematic searching for countries’ supply chain activities in documents and web pages. Cross-sectional multiple regression analysis was used to assess the association between the number of agricultural and manufacturing TTLCs and the Tobacco Industry Interference score, and the degree to which these were mediated by tobacco control, good governance, and economic importance of tobacco.

Results
TTLC supply chain activity had global reach. As the number of TTLCs with tobacco growing and manufacturing activities rose, interference increased significantly. Interference was associated with poorer tobacco control. The association with more TTLCs undertaking final product manufacturing was related to higher-value tobacco exports but was not related to tobacco making a bigger contribution to the economy.

Conclusions
TTLCs continue to control the global tobacco supply chain through their globe-spanning subsidiaries. The presence of TTLCs in a country is associated with political interference. Countries should consider their participation in the tobacco supply chain alongside the understanding that they are likely to cede political power to TTLCs, potentially undermining the health of their populations.

Implications
Tobacco control research has traditionally concentrated on the demand side of tobacco. Our results lend support to calls for more research on the supply of tobacco. Governments should require tobacco companies to provide detailed, up-to-date information in an easily accessible format on in-country supply chain activities. Policymakers should take the likelihood of political interference in health and environmental policy into account when making decisions about foreign direct investment offered by the tobacco industry.
Molecular Bulkiness of a Single Amino Acid in the F1 α-Subunit Determines the Robustness of Cyanobacterial ATP Synthase
Authors: Machida Akito , Kondo Kumiko , Wakabayashi Ken-ichi , Tanaka Kan , Hisabori Toru 
Published: September 2023

Abstract
Cyanobacteria are promising photosynthetic organisms owing to their ease of genetic manipulation. Among them, Synechococcus elongatus UTEX 2973 exhibits faster growth, higher biomass production efficiency and more robust stress tolerance compared with S. elongatus PCC 7942. This is due to specific genetic differences, including four single-nucleotide polymorphisms (SNPs) in three genes. One of these SNPs alters an amino acid at position 252 of the FoF1 ATP synthase α-subunit from Tyr to Cys (αY252C) in S. elongatus 7942. This change has been shown to significantly affect growth rate and stress tolerance, specifically in S. elongatus. Furthermore, experimental substitutions with several other amino acids have been shown to alter the ATP synthesis rate in the cell. In the present study, we introduced identical amino acid substitutions into Synechocystis sp. PCC 6803 at position 252 to elucidate the amino acid’s significance and generality across cyanobacteria. We investigated the resulting impact on growth, intracellular enzyme complex levels, intracellular ATP levels and enzyme activity. The results showed that the αY252C substitution decreased growth rate and high-light tolerance. This indicates that a specific bulkiness of this amino acid’s side chain is important for maintaining cell growth. Additionally, a remarkable decrease in the membrane-bound enzyme complex level was observed. However, the αY252C substitution did not affect enzyme activity or intracellular ATP levels. Although the mechanism of growth suppression remains unknown, the amino acid at position 252 is expected to play an important role in enzyme complex formation.
A multitiered haplotype strategy to enhance phased assembly and fine mapping of a disease resistance locus
Authors: Zou Cheng , Sapkota Surya , Figueroa-Balderas Rosa , Glaubitz Jeff , Cantu Dario , Kingham Brewster F , Sun Qi , Cadle-Davidson Lance 
Published: September 2023

Abstract
Fine mapping of quantitative trait loci (QTL) to dissect the genetic basis of traits of interest is essential to modern breeding practice. Here, we employed a multitiered haplotypic marker system to increase fine mapping accuracy by constructing a chromosome-level, haplotype-resolved parental genome, accurate detection of recombination sites, and allele-specific characterization of the transcriptome. In the first tier of this system, we applied the preexisting panel of 2,000 rhAmpSeq core genome markers that is transferable across the entire Vitis genus and provides a genomic resolution of 200 kb to 1 Mb. The second tier consisted of high-density haplotypic markers generated from Illumina skim sequencing data for samples enriched for relevant recombinations, increasing the potential resolution to hundreds of base pairs. We used this approach to dissect a novel Resistance to Plasmopara viticola-33 (RPV33) locus conferring resistance to grapevine downy mildew, narrowing the candidate region to only 0.46 Mb. In the third tier, we used allele-specific RNA-seq analysis to identify a cluster of 3 putative disease resistance RPP13-like protein 2 genes located tandemly in a nonsyntenic insertion as candidates for the disease resistance trait. In addition, combining the rhAmpSeq core genome haplotype markers and skim sequencing–derived high-density haplotype markers enabled chromosomal-level scaffolding and phasing of the grape Vitis × doaniana ‘PI 588149’ assembly, initially built solely from Pacific Biosciences (PacBio) high-fidelity (HiFi) reads, leading to the correction of 16 large-scale phasing errors. Our mapping strategy integrates high-density, phased genetic information with individual reference genomes to pinpoint the genetic basis of QTLs and will likely be widely adopted in highly heterozygous species.
Stripe rust effector Pst21674 compromises wheat resistance by targeting transcription factor TaASR3
Authors: Zheng Peijing , Liu Mengxue , Pang Lijing , Sun Ruyi , Yao Mohan , Wang Xiaojie , Kang Zhensheng , Liu Jie 
Published: September 2023

Abstract
Pathogens compromise host defense responses by strategically secreting effector proteins. However, the molecular mechanisms by which effectors manipulate disease-resistance factors to evade host surveillance remain poorly understood. In this study, we characterized a Puccinia striiformis f. sp. tritici (Pst) effector Pst21674 with a signal peptide. Pst21674 was significantly upregulated during Pst infections in wheat (Triticum aestivum L.) and knocking down Pst21674 by host-induced gene silencing led to reduced Pst pathogenicity and restricted hyphal spread in wheat. Pst21674 interaction with the abscisic acid-, stress-, and ripening-induced protein TaASR3 was validated mainly in the nucleus. Size exclusion chromatography, bimolecular fluorescence complementation, and luciferase complementation imaging assays confirmed that TaASR3 could form a functional tetramer. Virus-induced gene silencing and overexpression demonstrated that TaASR3 contributes to wheat resistance to stripe rust by promoting accumulation of reactive oxygen species and cell death. Additionally, transcriptome analysis revealed that the expression of defense-related genes was regulated in transgenic wheat plants overexpressing TaASR3. Interaction between Pst21674 and TaASR3 interfered with the polymerization of TaASR3 and suppressed TaASR3-mediated transcriptional activation of defense-related genes. These results indicate that Pst21674 serves as an important virulence factor secreted into the host nucleus to impede wheat resistance to Pst, possibly by targeting and preventing polymerization of TaASR3.
Cuscuta campestrisfine-tunes gene expression during haustoriogenesis as an adaptation to different hosts
Authors: Bawin Thomas , Didriksen Alena , Faehn Corine , Olsen Stian , Sørensen Iben , Rose Jocelyn K C , Krause Kirsten 
Published: September 2023

AbstractThe Cuscuta genus comprises obligate parasitic plants that have an unusually wide host range. Whether Cuscuta uses different infection strategies for different hosts or whether the infection strategy is mechanistically and enzymatically conserved remains unknown. To address this, we investigated molecular events during the interaction between field dodder (Cuscuta campestris) and two host species of the Solanum genus that are known to react differently to parasitic infection. We found that host gene induction, particularly of cell wall fortifying genes, coincided with a differential induction of genes for cell wall degradation in the parasite in the cultivated tomato (Solanum lycopersicum) but not in a wild relative (Solanum pennellii). This indicates that the parasite can adjust its gene expression in response to its host. This idea was supported by the increased expression of C. campestris genes encoding an endo-β-1,4-mannanase in response to exposure of the parasite to purified mono- and polysaccharides in a host-independent infection system. Our results suggest multiple key roles of the host cell wall in determining the outcome of an infection attempt.
Improvement of sleep and pain with lemborexant administration in patients with chronic pain: a retrospective observational study
Authors: Ueno Kyohei , Sato Hitoaki , Nomura Yuki , Obata Norihiko , Mizobuchi Satoshi 
Published: September 2023

Abstract

Objective
Patients with chronic pain often have sleep disturbances, and many patients receive sleep medications in addition to analgesics. Although there have been scattered reports of negative pain–sleep interactions, only a few reports have investigated the efficacy of sleep medication interventions in patients with chronic pain for improving sleep disturbances and reducing pain. We retrospectively examined whether lemborexant, an orexin receptor antagonist, is effective in improving sleep disturbances and reducing pain in patients with chronic pain. This study was approved by the Ethics Committee of our hospital.

Methods
The subjects were 26 patients with chronic pain undergoing treatment at our pain clinic between July 2021 and March 2022, who had been diagnosed with insomnia, with an Athens Insomnia Scale (AIS) score of ≥6 and had been started on lemborexant. The AIS score and pain score (Numeric Rating Scale [NRS]) before and after 2 and 4 weeks of starting lemborexant were investigated.

Results
Patients who were already taking other sleep medications, such as benzodiazepines were switched to 5 mg of lemborexant after all the other sleep medications were discontinued. Those who had not yet used sleeping pills were started on 5 mg of lemborexant. During the study course, the dose of lemborexant was adjusted at the discretion of the attending physician, based on improvement of insomnia symptoms and secondary symptoms, such as daytime sleepiness and lightheadedness. The study finally included 21 patients, excluding 5 who could not continue taking lemborexant due to side effects, such as lightheadedness. The AIS scores significantly improved, decreasing from baseline (mean ± standard deviation: 12.5 ± 4.9) to 2 weeks (7.8 ± 3.1) and 4 weeks (5.3 ± 2.9) after the start of lemborexant. No significant difference was observed in the degree of improvement in sleep disturbance between patients with or without previous sleep medications, and there was also no statistically significant improvement in the NRS score before (6.1 ± 2.7) and after 2 weeks (5.5 ± 2.3) and 4 weeks (5.9 ± 2.2) from treatment initiation.