bluuebunny/crossref_metadata_2025_split huggingface dataset:
The HIGH EXPRESSION OF OSMOTICALLY RESPONSIVE GENE15–HISTONE DEACETYLASE9 complex associates with HYPONASTIC LEAVES 1 to modulate microRNA expression in response to abscisic acid signaling
Authors: Park Junghoon , Giudicatti Axel J , Bader Zein Eddin , Han Min Kyun , Møller Christian , Arce Agustin L , Xu Zheng-Yi , Yang Seong Wook , Manavella Pablo A , Yun Dae-Jin 
Published: May 2023

AbstractThe regulation of microRNA (miRNA) biogenesis is crucial for maintaining plant homeostasis under biotic and abiotic stress. The crosstalk between the RNA polymerase II (Pol-II) complex and the miRNA processing machinery has emerged as a central hub modulating transcription and cotranscriptional processing of primary miRNA transcripts (pri-miRNAs). However, it remains unclear how miRNA-specific transcriptional regulators recognize MIRNA loci. Here, we show that the Arabidopsis (Arabidopsis thaliana) HIGH EXPRESSION OF OSMOTICALLY RESPONSIVE GENE15 (HOS15)–HISTONE DEACETYLASE9 (HDA9) complex is a conditional suppressor of miRNA biogenesis, particularly in response to abscisic acid (ABA). When treated with ABA, hos15/hda9 mutants show enhanced transcription of pri-miRNAs that is accompanied by increased processing, leading to overaccumulation of a set of mature miRNAs. Moreover, upon recognition of the nascent pri-miRNAs, the ABA–induced recruitment of the HOS15–HDA9 complex to MIRNA loci is guided by HYPONASTIC LEAVES 1 (HYL1). The HYL1–dependent recruitment of the HOS15–HDA9 complex to MIRNA loci suppresses expression of MIRNAs and processing of pri-miRNA. Most importantly, our findings indicate that nascent pri-miRNAs serve as scaffolds for recruiting transcriptional regulators, specifically to MIRNA loci. This indicates that RNA molecules can act as regulators of their own expression by causing a negative feedback loop that turns off their transcription, providing a self-buffering system.
Factors affecting college students’ attitudes towards carpooling
Authors: Li Linchao , Zhang Huali , Gan Zuoxian 
Published: May 2023

Abstract
As a sustainable mode of travel, carpooling is an effective solution to environmental and energy problems, but it has not been fully utilized. The research on carpooling attitudes, especially for college students, still needs to be improved. In response to this problem, this paper aims to investigate the effect of personal attributes, travel attributes, attitude attributes and other attributes of students on carpooling attitudes in China. To collect the data, an online questionnaire survey was implemented at Shenzhen University, and 514 students participated. Based on the samples, the multinomial logit model is used to explore the contribution of all variables. The model results showed that personal attributes such as sex and age had no significant effect on the attitude of college students towards carpooling. The safety of carpooling and its high cost make college students have a neutral attitude towards it. The most concerning factor for college students in carpooling is comfort. These findings can provide valuable suggestions for measures to be taken in response to different attributes affecting students’ attitudes and are particularly important for the university and government to make optimal decisions to motivate students to select carpooling.
Goal-Pursuit Tendencies and Retirement Planning: A Time-Lagged and Multi-Dimensional Investigation
Authors: Liu Pei , Thrasher Gregory R , Ye Yanhua , Shi Junqi 
Published: May 2023

Abstract
Existing studies regarding retirement planning have suggested that demographic variables, retirement-related characteristics, and work-related factors are closely associated with retirement planning. However, the importance of individual goal-pursuit tendencies within a multi-dimensional framework of retirement planning has been overlooked. As retirement planning is conceptualized as a multi-faceted and goal-directed behavior, the absence of these concepts within the literature presents an important theoretical and empirical gap. Drawing upon the dual-process model of coping, the current study examined the incremental effects of tenacious goal pursuit (TGP) and flexible goal adjustment (FGA) on four dimensions of retirement planning (i.e., financial planning, health planning, interpersonal/leisure planning, and work planning). Data from 377 Chinese older workers at two time points showed that FGA rather than TGP was positively associated with financial, health, and interpersonal/leisure planning. Further, FGA demonstrated incremental predictive validity over TGP. This study contributes to the retirement planning literature by investigating how older adults’ goal-related dispositions may impact their retirement planning in different arenas. Practical implications of our findings, limitations, and future research directions are also discussed.
The Commercial Validation of Three Tomato Brown Rugose Fruit Virus Assays
Authors: Eads Alex , Groth-Helms Deborah , Davenport Bryant , Cha Xian , Li Rugang , Walsh Coilin , Schuetz Keith 
Published: May 2023

Tomato brown rugose fruit virus (ToBRFV) was first described infecting tomatoes in 2015 after breaking tobamovirus resistance in production cultivars. As an emerging pathogen, ToBRFV commercial assays were developed and subsequently validated. Agdia commercialized three ToBRFV assays: ELISA, ImmunoStrip, and AmplifyRP XRT. The ToBRFV ELISA is a serological method utilizing monoclonal antibodies designed for use as a high-throughput laboratory tool. The ToBRFV ImmunoStrip is a serological lateral flow device utilizing monoclonal antibodies designed for use as a field-screening tool. The ToBRFV XRT is a molecular method utilizing recombinase polymerase amplification designed to be a hybrid field-screening/laboratory tool with PCR-like sensitivity. The performance criteria and validation requirements for the three assays were chosen and evaluated based on each product's fitness for purpose. The ELISA and ImmunoStrip both have an analytical sensitivity of 64 to 320 pg/ml and an analytical specificity inclusive of all tested ToBRFV isolates with cross-reactions to tobacco mosaic virus, tomato mosaic virus, and tomato mottle mosaic virus. The XRT has an analytical sensitivity of 15 fg/µl and an analytical specificity inclusive of all tested ToBRFV isolates with no cross-reactions. All three assays have both a diagnostic sensitivity and specificity of 100%. Reliable diagnostic and analytical data are necessary for commercial assays to be both accepted by and useful to diagnosticians, researchers, and breeders. Without adequate validation, false results and/or false reporting can occur. In the absence of formal validation requirements in the global plant pathogen diagnostic community, Agdia commercialized ToBRFV ELISA, ImmunoStrip, and AmplifyRP XRT assays and evaluated them based on publicly available and internal performance criteria. [Formula: see text] Copyright © 2023 The Author(s). This is an open access article distributed under the CC BY-NC-ND 4.0 International license .
Prevalence of psoriatic arthritis in Chinese population with psoriasis: A multicenter study conducted by experienced rheumatologists
Authors: Zhang Hua , Chen Miao , Cui Ran , Li Xia , Yan Kexiang , Chen Lihong , Zhang Zhenghua , Yu Ning , Bi Xinling , Deng Hui , Ding Yangfeng , Huang Qiong , Dai Sheng-Ming 
Published: May 2023

Abstract

Background:
Reports on the prevalence of psoriatic arthritis (PsA) among Chinese patients with psoriasis are very limited. This study, conducted by rheumatologists, estimated the prevalence of PsA in a large number of Chinese patients with psoriasis.

Methods:
Consecutive patients with a confirmed diagnosis of psoriasis attending nine dermatology clinics in five hospitals were recruited. All psoriasis patients were asked to complete a questionnaire comprising 16 questions to identify possible cases of PsA. All patients with one or more positive answers to the questionnaire were evaluated by two experienced rheumatologists.

Results:
A total of 2434 psoriasis patients, including 1561 males and 873 females, were enrolled. Both the questionnaire and rheumatologists' examinations were completed in the dermatology clinics. The results identified 252 patients with PsA, comprising 168 males and 84 females. The overall prevalence of PsA among psoriasis patients was 10.4% (95% confidence interval [95% CI], 9.1%–11.7%). By sex, the prevalence was 10.8% (95% CI, 9.2%–12.5%) for males and 9.6% (95% CI, 7.7%–11.9%) for females and there was no significant sex difference in the prevalence of PsA (P = 0.38). Of the 252 PsA patients, 125 (49.6%, 95% CI, 41.3%–59.1%) were newly diagnosed by rheumatologists. Consequently, the prevalence of undiagnosed PsA among psoriasis patients was 5.2% (95% CI, 4.4%–6.2%).

Conclusion:
The prevalence of PsA in the Chinese population with psoriasis is about 10.4%, which is almost double that of previous reports in the Chinese population, but lower than that in Caucasians.
A Systematic Review and Meta-analysis of Clinical Outcomes in Autologous Breast Reconstruction Using Internal Mammary Artery Perforators as Recipient Vessels
Authors: Fernandez-Diaz Oscar F. , Christopoulos Georgios , Griffiths Matthew 
Published: May 2023

Background:
Recipient vessel selection is vital for successful autologous free-flap breast reconstruction. Internal mammary artery perforators have gained interest as a recipient vessel option. However, previous studies on their microsurgical safety and efficacy are limited and inconsistent. Thus, we conducted a systematic review and meta-analysis to assess the safety and effectiveness of using internal mammary artery perforators as recipient vessels in breast reconstruction.

Methods:
The protocol has been previously published in PROSPERO (CRD42020190020). The PubMed, Scopus, Web of Science, and PROSPERO databases were searched. Two independent reviewers evaluated the articles for inclusion in the study. Study quality was assessed using the Newcastle-Ottawa Scale and the MINORS instrument (Methodological Index for Non-Randomized Studies).

Results:
Of the 361 articles screened, 13 studies were included (313 patients with 318 flaps; 223 unilateral, 31 bilateral, mean average age 51.2 and mean BMI 27.8 ± 1.9). The mean overall success rate was 99.8%, the pooled surgical success rate was 100% [95% confidence interval (CI): 97%–100%], and the overall rate of complications was 11% (95% CI: 7%–18%). The most common complication was vascular-related to microanastomoses, with an incidence of 5% (95% CI: 2%–10%). The fat necrosis rate was 3% (95% CI: 2%–6%).

Conclusions:
This study verified that internal mammary artery perforator vessels are reliable in breast reconstruction, with a high success rate and a relatively low complication rate. Moreover, in selected microsurgical breast reconstruction patients, internal mammary artery perforators may be the primary recipient vessel choice over the internal mammary artery or thoracodorsal vessels.
Sleep-related factors associated with masked hypertension: the Nagahama study
Authors: Tabara Yasuharu , Matsumoto Takeshi , Murase Kimihiko , Setoh Kazuya , Kawaguchi Takahisa , Nakayama Takeo , Wakamura Tomoko , Hirai Toyohiro , Chin Kazuo , Matsuda Fumihiko 
Published: May 2023

Objectives:
Masked hypertension, which is characterized by out-of-office hypertension but normal office blood pressure, is a risk factor for cardiovascular disease. However, the factors that contribute to masked hypertension are unclear. We aimed to determine the involvement of sleep-related characteristics in masked hypertension.

Methods:
The study included 3844 normotensive (systolic/diastolic blood pressure < 140/90 mmHg) community residents with no antihypertensive drug use at baseline (mean age 54.3 years). Home morning and evening blood pressure, oxygen desaturation during sleep (pulse oximetry), and sleep efficiency (actigraphy) were measured for 1 week. The number of nocturnal urinations during this period was obtained using a sleep diary.

Results:
Masked hypertension (mean morning and evening blood pressure ≥135/85 mmHg) was detected in 11.7% of study participants, and 79.0% of the participants with masked hypertension had sleep hypertension (≥120/70 mmHg). Multinominal logistic regression analysis identified different factors involved in masked hypertension with and without sleep hypertension; factors for masked hypertension with sleep hypertension included the frequency of at least 3% oxygen desaturation (coefficient = 0.038, P = 0.001), nocturia (coefficient = 0.607, P < 0.001), and carotid intima-media thickness (coefficient = 3.592, P < 0.001). Only carotid intima-media thickness and measurement season were associated with masked hypertension without sleep hypertension. Low sleep efficiency was associated with isolated sleep hypertension but not masked hypertension.

Conclusion:
Sleep-related factors associated with masked hypertension differed depending on the presence of sleep hypertension. Sleep-disordered breathing and nocturnal urination frequency may help identify individuals who need home blood pressure monitoring.
177Lu-DOTATATE Therapy: Effects of Water Intake and Drinking Duration on Patient Peripheral Dose Equivalent Rates
Authors: Ni Yudan , Ye Qing , Liu Xiangnan , Wang Ke , Yu Fei , Fu Jingjing , Wang Feng , Li Lin 
Published: May 2023

Abstract
This study investigated the amount and duration of water consumption in neuroendocrine tumor patients after receiving 177Lu-DOTATATE radionuclide therapy. We recruited 39 patients with neuroendocrine tumors, all of whom were treated with 177Lu-DOTATATE radionuclide while in the nuclear medicine ward of a tertiary hospital in Nanjing from January 2021 to April 2022. We conducted a cross-sectional survey to investigate their drinking times, water consumption, and urine volumes at 0 min, 30 min, 60 min, 2 h, 24 h, and 48 h after radionuclide treatment. At each timepoint, their radiation dose equivalent rates were monitored at 0 m, 1 m, and 2 m from the middle abdomen. f at 24 h were significantly lower than those at 0 min, 30 min, 60 min, and 2 h (all p < 0.05). The dose equivalent rates at 48 h were significantly lower than those at 24 h (all p < 0.05). At 1 m or 2 m from the patient, the dose equivalent rate gradually decreased at all six timepoints and was significantly different between groups (P < 0.05). To achieve lower radiation doses, there was a correlation with 24 h water consumption (P < 0.05) but no correlation with 48 h water consumption (P > 0.05); there were lower peripheral dose equivalents for patients when 24 h water consumption was no less than 2,750 mL. Patients with neuroendocrine tumors should drink at least 2,750 mL of water 24 h after treatment with 177Lu-DOTATATE radionuclides. Drinking water in the first 24 h after treatment is more critical to reduce the peripheral dose equivalent, which can accelerate the reduction of peripheral radiation dose equivalent in early patients.
Mental Health Impact of Keratoconus: A Systematic Review
Authors: Durakovic Emily , Kandel Himal , Watson Stephanie L. 
Published: May 2023

Purpose:The aim of this study was to investigate the impact of keratoconus on the mental health and emotional well-being of affected patients.Methods:A literature search was conducted according to the PRISMA guidelines. Databases searched included MEDLINE, PubMed, EMBASE, Scopus, Web of Science, Cochrane, and PsycINFO. Articles were included if they were a primary study that investigated mental health or emotional quality-of-life outcomes in patients with keratoconus.Results:Thirty-one articles met inclusion criteria of a total yield of 444. Most studies reported keratoconus to have a negative effect on mental health or emotional well-being. Worsening mental health scores correlated with lower visual acuity (VA) in the better eye, lower VA in the worse eye, increasing ocular asymmetry, and worsening disease severity. Mental health impacts were often reported to be disproportionate to that of the effects on VA. Over time mental health outcomes improved, suggesting stabilization of disease and patient acceptance.Conclusions:Patients with keratoconus may suffer detriments to mental health despite having relatively good VA. Understanding and acceptance of their disease may assist in assuaging mental health concerns. Further work may be needed to investigate whether benefit exists in routine mental health screening of patients with keratoconus.
Improving Data Integrity and Quality From Online Health Surveys of Women With Infant Children
Authors: Walker Lorraine O. , Murry Nicole , Longoria Kayla D. 
Published: May 2023

Background
Online surveys have proven to be an efficient method to gather health information in studies of various populations, but these are accompanied by threats to data integrity and quality. We draw on our experience with a nefarious intrusion into an online survey and our efforts to protect data integrity and quality in a subsequent online survey.

Objectives
We aim to share lessons learned regarding detecting and preventing threats to online survey data integrity and quality.

Methods
We examined data from two online surveys we conducted, as well as findings of others reported in the literature, to delineate threats to and prevention strategies for online health surveys.

Results
Our first survey was launched inadvertently without available security features engaged in Qualtrics, resulting in a number of threats to data integrity and quality. These threats included multiple submissions, often within seconds of each other, from the same internet protocol (IP) address; use of proxy servers or virtual private networks, often with suspicious or abusive IP address ratings and geolocations outside the United States; and incoherent text data or otherwise suspicious responses. After excluding fraudulent, suspicious, or ineligible cases, as well as cases that terminated before submitting data, 102 of 224 (45.5%) eligible survey respondents remained with partial or complete data. In a second online survey with security features in Qualtrics engaged, no IP addresses were associated with any duplicate submissions. To further protect data integrity and quality, we added items to detect inattentive or fraudulent respondents and applied a risk scoring system in which 23 survey respondents were high risk, 16 were moderate risk, and 289 of 464 (62.3%) were low or no risk and therefore considered eligible respondents.

Discussion
Technological safeguards, such as blocking repeat IP addresses and study design features to detect inattentive or fraudulent respondents, are strategies to support data integrity and quality in online survey research. For online data collection to make meaningful contributions to nursing research, it is important for nursing scientists to implement technological, study design, and methodological safeguards to protect data integrity and quality and for future research to focus on advancing data protection methodologies.
Development and Internal Validation of a Multivariable Prediction Model to Predict Repeat Attendances in the Pediatric Emergency Department
Authors: Seers Tim , Reynard Charles , Martin Glen P. , Body Richard 
Published: May 2023

ObjectiveUnplanned reattendances to the pediatric emergency department (PED) occur commonly in clinical practice. Multiple factors influence the decision to return to care, and understanding risk factors may allow for better design of clinical services. We developed a clinical prediction model to predict return to the PED within 72 hours from the index visit.MethodsWe retrospectively reviewed all attendances to the PED of Royal Manchester Children's Hospital between 2009 and 2019. Attendances were excluded if they were admitted to hospital, aged older than 16 years or died in the PED. Variables were collected from Electronic Health Records reflecting triage codes. Data were split temporally into a training (80%) set for model development and a test (20%) set for internal validation. We developed the prediction model using LASSO penalized logistic regression.ResultsA total of 308,573 attendances were included in the study. There were 14,276 (4.63%) returns within 72 hours of index visit. The final model had an area under the receiver operating characteristic curve of 0.64 (95% confidence interval, 0.63–0.65) on temporal validation. The calibration of the model was good, although with some evidence of miscalibration at the high extremes of the risk distribution. After-visit diagnoses codes reflecting a nonspecific problem (“unwell child”) were more common in children who went on to reattend.ConclusionsWe developed and internally validated a clinical prediction model for unplanned reattendance to the PED using routinely collected clinical data, including markers of socioeconomic deprivation. This model allows for easy identification of children at the greatest risk of return to PED.
The Management of Low-Risk Myelodysplastic Syndromes—Current Standards and Recent Advances
Authors: Randall Michael P. , DeZern Amy E. 
Published: May 2023

Abstract
The myelodysplastic syndromes (MDSs) are a heterogeneous group of hematologic neoplasms with varied natural histories and prognoses. Specific to this review, treatment of low-risk MDS most often focuses on improving quality of life by correcting cytopenias, as opposed to urgent disease modification to avoid acute myeloid leukemia. These treatments include transfusion support with iron chelation when necessary, growth factors including novel maturation agents such as luspatercept, lenalidomide for del(5q) disease, and, increasingly, low-dose hypomethylating agents. Recent advances in the understanding of the genetic lesions that drive MDS have prompted a reassessment of how low-risk disease is defined and helped to identify a subset of low-risk MDS patients who may benefit from a more aggressive treatment paradigm, including hematopoietic stem cell transplantation.
Spectrum From Clonal Hematopoiesis to Myelodysplastic Neoplasm/Syndromes and Other Myeloid Neoplasms
Authors: Xie Zhuoer , Chen Evan C. , Mendez Lourdes M. , Komrokji Rami , Zeidan Amer M. 
Published: May 2023

Abstract
Clonal hematopoiesis (CH) confers a high risk of aging-related diseases and hematologic malignancy. There are still significant knowledge gaps in identifying high-risk patients with CH and managing such patients. In this review, we focus on 3 areas: (1) the natural history of CH; (2) the risks of progression of CH, including CH of indeterminate potential, clonal cytopenia of undetermined significance, and therapy-related CH, to myeloid malignancy; and (3) the challenges and unmet needs of CH management and research.