bluuebunny/crossref_metadata_2025_split huggingface dataset:
Regulating Neuroinflammatory Biomarkers in Nitroglycerine induced Migraine in Rats: A Therapeutic role of Chrysanthemum indicum extract
Authors: Rushendran Rapuru , Vellapandian Chitra , Kaliappan Ilango 
Published: June 2023

Abstract
Objective
Chronic migraine (CM) is characterised by unilateral/bilateral pulsatile headaches at least 15 days per month. Central sensitization can be demonstrated by a prolonged increase in trigeminal nucleus caudalis (TNC) neuron activity in response to painful stimuli. Scientists are continually researching migraine treatments, hoping herbal remedies may work better. Network pharmacological approach was used to assess Chrysanthemum indicum (CIHE) leaf hydroalcoholic extract to evaluate antimigraine activity.
Methods
We investigated specific genes involved in the migraine, extracted gene ontology, biological pathways, and protein-protein interaction analysis were determined with the screened 61 common genes by network pharmacological approach added with docking analysis. In vivo studies have been carried out with CD1 Mice (25-30g) randomly made into five groups. CIHE is prepared to evaluate antimigraine activity. MMP9, TNF-α, NFkB, IL-1β, CGRP, and iNOS were evaluated after administration of Nitroglycerine (10mg/kg; i.p).
Results
The elevated protein levels were significantly reduced with the treatment of CIHE (200mg/kg and 400mg/kg; p.o). Additionally histological and western blot analysis confirmed the reduction of specified proteins in the brain as well as blood. Docking analysis revealed that 1,5-Bis(4-dimethylaminophenyl)-1,4-pentadien-3-one (-10.3 KJ/mol), Naphthalene-1-carboxylic acid 4-formyl-phenyl ester (-9.7KJ/mol), and 3-(3-Nitrophenyl)-2'-acrylonaphthone (-9.7KJ/mol) are shown highest binding affinity.
Interpretation:
CIHE may attain antimigraine activity through suppression of neuroinflammatory cytokines by the natural extract of Chrysanthemum indicum in a dose dependent manner and isolation of hit molecules from the CIHE is required for further investigation.
Molecular Profiling reveals Tumor Immune Microenvironment Heterogeneity of Concurrent Lung Adenocarcinoma and Squamous Cell Carcinoma in Multiple Primary Lung Cancers
Authors: Zhang Yajie , Zhang Jiahao , Dong Dong , Cao Yuqin , Chen Xiang , Liu Di , Cheng Xueyan , Sun Debin , Li Hecheng 
Published: June 2023

Abstract
Background: Over the past decade, the annual incidence of multiple primary lung cancers (MPLC) has increased. There is a group of pathologically different patients with MPLC presenting as mostly having concurrent lung adenocarcinoma (ADC) and squamous cell carcinoma (SQCC). Their molecular profiles and tumor immune microenvironment (TIME) remain unknown. We aimed to clarify these factors in this patient group.
Methods: We performed mutational, biomarker, and neoantigen analyses using whole-exome sequencing (WES) and differential, enrichment,and TIME analyses using RNA sequencing (RNA-Seq) for five patients with concurrent ADC and SQCC to analyze their mutational profile, expression profile, and TIME features.
Results: WES of 10 lesions (five ADC and five SQCC) in five concurrent ADC and SQCC patients showed that the most frequently mutated genes in both groups were TTN, TP53, WDFY3, EGFR, ZFHX4 and TTC40, and there were no significantly different mutated genes in the comparative analysis of mutation frequencies between both groups. There were no significant differences inneoantigen analysis, including tumor mutational burden, tumor neoantigen burden, and mutant allele tumor heterogeneity. HLA genotype analysis indicated that three of five (60%) patients carried the HLA-I class B62 supertype, and seven of 10 (70%) lesions had loss of HLA heterozygosity (HLA-LOH).
RNA-Seq revealed that 2231 genes were differently expressed between the ADC and SQCC groups. GSVA based on the molecular signature database (MsigDB) showed that 12 gene sets were significantly differentially expressed between the ADC and SQCC groups (p < 0.05), with four gene sets relevant to squamous cell features upregulated in the SQCC group and eight metabolism-related gene sets upregulated in ADC. GO/KEGG analysis of immune function-related pathways showed that the interleukin-12 family-related signaling pathways, TP53 and TLR2/4 signaling pathways were upregulated in the ADC group. Co-expression analysis of TIME revealed that tumor infiltrating immune cells were significantly more enriched and diverse, with tumor-infiltrating lymphocytes scoring significantly higher (p = 0.047) in ADC. Specifically, CD8+ T cells, exhausted CD8+ cells, and macrophages infiltrated significantly more frequently in ADC (p < 0.05). Moreover, immunocostimulator expression (p = 0.034) and tertiary lymphoid structure score (p = 0.017) were significantly higher in ADC than in SQCC.
Conclusion: In patients with concurrent ADC and SQCC MPLC, the transcriptomic profiles and TIME features were quite different between ADC and SQCC lesions. ADC lesions exhibited a more active TIME than SQCC lesions.
SVPC-LDA: A Hybridised Feature Extraction Approach for Chronic Kidney Disease Dataset
Authors: Gulati Vineeta , Raheja Neeraj , Rana Arun Kumar , Islam Sardar M N , Alsubaie Najah , Alqahtani Mohammed S. , Abbas Mohamed , Soufiene Ben Othman 
Published: June 2023

Abstract

High-dimensional data is a major challenge for a high-quality machine learning model. Feature extraction is the most common technique offered in the literature to reduce the amount of data. Moreover, relevant feature extraction leads to a more efficient and reliable classification system through machine learning (ML). In this study, the authors introduce Singular Value Principal Component Linear Discriminant Analysis (SVPC-LDA), a hybrid dimensionality reduction technique that combines the three most popular feature extraction methods: Linear Discriminant Analysis (LDA), Singular Value Decomposition (SVD), and Principal Component Analysis (PCA). The proposed technique was evaluated using Gaussian NB (Naïve Bayes), K Neighbors, Gaussian Process, Linear SVC (Support Vector Classifier), SGD (Stochastic Gradient Descent), and Passive Aggressive Classifiers on the standard chronic kidney disease dataset. In addition, the efficiency of SVPC-LDA was compared with all features and with existing PCA, SVD, ICA, and LDA methods. In addition, the effectiveness of the hybridized SVPC-LDA technique was measured on a high scale, with achieved values of 98.75% accuracy, 98.07% sensitivity, 96.55% precision, 100% specificity, and 99.03% AUC. In addition, dimensionality was reduced by 15% and RMSE by 40.60%, which is better than the techniques found in the literature.
Rapid determination of warfarin in biological samples: Experimental and theoretical studies of Pt-doped CdTe QDs as fluorescent probes
Authors: Safari Meysam , Ehzari Hosna , Yousif Azeezah Yaseen , Samimi Mohsen 
Published: June 2023

Abstract
This research is focused on introducing a facile and safe fluorescence sensor for the sensitive and selective detection of warfarin in biological samples. The synthesis of the fluorescence sensor probe was easily performed with doping metal ions of the Pt to CdTe quantum dots (Pt:CdTe QDs). The morphological and optical properties of the synthesized Pt:CdTe quantum dots were characterized as FTIR spectroscopy, TEM, UV visible absorption and EDX. The interaction patterns of warfarin with CdTe QDs were investigated by the quantum chemical method and compared with experimental results. The proposed quantum dots exhibited a blue luminescence with a 28.8% quantum yield. Pt:CdTe QDs were used as the fluorescence probe to assay warfarin. This procedure is based on "off" fluorescence of quantum dots in the presence of different concentrations of warfarin. Under optimal conditions, the fluorescence sensor probe could detect the concentration of warfarin with a wide linear range of 2–100 µM and a detection limit (S/N = 3) of 0.75 µM. Results of real sample analysis by fluorescent nanoprobe displayed that this probe could be the potential alternative tool for warfarin drug detection in biological samples.
Association between early mobilization and functional outcomes in patients with aneurysmal subarachnoid hemorrhage: A Multicenter, retrospective, propensity score-matched study
Authors: Takara Hikaru , Suzuki Shota , Satoh Shuhei , Abe Yoko , Miyazato Shohei , Kohatsu Yoshiki , Minakata Shin , Moriya Masamichi 
Published: June 2023

Abstract
Background
Early mobilization is important for promoting functional recovery and preventing complications in patients with aneurysmal subarachnoid hemorrhage (aSAH). However, the efficacy of early mobilization in patients with aSAH remains unclear. This study aimed to investigate the association between early mobilization and functional outcomes in patients with aSAH.
Methods
This multicenter retrospective study was conducted in Japan, including patients with aSAH who received physical therapy, with or without occupational therapy, from April 2014 to March 2019. The primary outcome was the modified Rankin Scale (mRS) score, with a favorable functional outcome defined as an mRS score of 0–2 and an unfavorable outcome with an mRS score of 3–5. Patients initiating walking training within 14 days of aSAH onset were classified into the early mobilization group, whereas those initiating training after 14 days were classified into the delayed mobilization group. Propensity score matching analysis was performed to assess the association between early mobilization and favorable outcomes.
Results
A total of 718 patients were screened and 450 eligible patients were identified. Before matching, 229 patients (50.9%) were in the early mobilization group and 221 (49.1%) were in the delayed mobilization group. After matching, each group consisted of 122 patients, and the early mobilization group exhibited a higher proportion of favorable outcomes than did the delayed mobilization group (81.1% vs. 52.5%; risk difference, 28.7%; 95% CI, 17.4–39.9; p < 0.001).
Conclusions
This multicenter retrospective study suggests that initiating walking training within 14 days of aSAH onset is associated with favorable outcomes.
The design features of digital games and their impact on language learning for EFL college students
Authors: Klimova Blanka , Al-Obaydi Liqaa Habeb , Tawafak Ragad M , Pikhart Marcel 
Published: June 2023

Abstract

Background
Digital games have been recognized as an effective tool to enhance language learning, and their design features, such as interactivity, challenge, or immersion, have been linked to successful language acquisition. The purpose of this research study is to explore some design features of digital games and show their impact on learning English by EFL college students.
Methods
The methodology was based on a questionnaire survey that was conducted in two countries (Iraq and the Czech Republic) among 160 undergraduate students of English as a foreign language.
Results
The findings of this study reveal that digital games have the potential to be effective tools for improving English language proficiency among EFL college students and their effectiveness is especially affected by design features, such as personalization and interactivity. In addition, language skills development and perceived usefulness are also important factors despite the fact that students do not associate the intention to learn a language and perceived ease of use of the games with learning.
Conclusions
By understanding these factors and design features, educators and game designers can create games that are more engaging and effective in promoting language learning among EFL college students.
Association of Residual Ductal Carcinoma In Situ with Breast Cancer Treatment Outcomes after Neoadjuvant Chemotherapy according to Hormone Receptor Status
Authors: Shin Eunju , Yoo Tae-Kyung , Kim Jisun , Chung Il Yong , Ko Beom Seok , Kim Hee Jeong , Lee Jong Won , Son Byung Ho , Lee Sae Byul 
Published: June 2023

Abstract
Purpose
This research aimed to clarify the impact of residual DCIS in surgical specimens obtained after NAC for breast cancer on the associated prognosis outcomes.
Methods
This retrospective study was performed on a cohort of 1,009 patients who achieved pCR following NAC for breast cancer and underwent subsequent breast surgery at a single institution between January 2008 and December 2019. Overall survival, local recurrence-free survival, distant metastasis-free survival, and disease-free survival of the residual and non-residual DCIS groups were the outcomes compared, with further subgroup analysis performed according to breast cancer type.
Results
Among the 1,009 patients who achieved pCR, 260 individuals (25.8%) presented with residual DCIS. Based on a median follow-up of 54.0 months, no significant differences in outcomes were observed between the two groups.
Patients with residual DCIS and hormone receptor-negative (HR-) breast cancer demonstrated a significant decrease in distant metastasis-free survival (p=0.030) compared to those without residual DCIS. In the HR+ cohort, no significant difference was observed between the two groups.
Multivariate analysis of the HR- cohort demonstrated a significant association between residual DCIS and an elevated risk for distant recurrence (hazard ratio = 2.3, 95% confidence interval = 1.01–5.20, p=0.047).
Conclusions
Residual DCIS following NAC may impact breast cancer outcomes, particularly with respect to the occurrence of distant metastasis in HR- patients. Therefore, clinicians must vigilantly monitor patients with residual DCIS after NAC, and further research is needed to expand our understanding of the clinical implications of residual DCIS.
WITHDRAWN: Symbolic representation of district wise climate change scenarios of West Bengal state, India, using Thornthwaite classification scheme
Authors: MALLICK ANKITA , DAS LALU , AKHTER JAVED , SAHA GAUTAM 
Published: June 2023

Abstract
Now-a-days, multi-level stakeholders starting from grass-root level farming community to Government level policy makers need to have adequate climate change information before hand to implement appropriate adaptation and mitigation strategies towards combating climate change at local scale. However, availability of climate change data is not adequate at district and block level. The present study has attempted to reveal how the district level climate has changed in last 120 years over 23 districts of West Bengal in India using Thornthwaite classification scheme (TCS). The change of climate from moist to dry or vice-versa from mean climatological period of 1901–1930 to 1991–2020 has been assessed and represented through four different English letters based on the calculated values of TCS. The study of many indicators showed that symbolic representations remain same in some districts but in several districts these vary according to changes of time period. The B1A'a'w2 symbol was used to express climatic type of PurbaBardhaman during the first three climatological eras, but it was altered to C2A'a'w2 for the last period, showing a shift in moisture index (Im) value. This reveals how the climatic type is continuously changing in North Bengal and South Bengal districts of West Bengal through the passage of time. Furthermore, it has been noted that while the majority of North Bengal districts have changed to drier conditions as their Im value has decreased recently, several South Bengal districts indicate moist conditions compared to the previous climatological periods with increases in moisture index (Im) value.
Beyond the Impact of Electronic Health Record Execution Issues on its Utilization: Pragmatic Approaches
Authors: Abbassi Hajar , Rahimi Bahlol , Jebraeily Mohamad , Alinejad Vahid , Nourani Aynaz 
Published: June 2023

Abstract

The Electronic Health Record (EHR) is a repository for storing health data and a network for exchanging these data. Today, the EHR is one of the key elements in the health system and has brought many benefits. These benefits reach their maximum when the system is properly executed and used. The purpose of this study was to investigate the impact of Iranian EHR (SEPAS) execution issues on its utilization. This was a quantitative-analytical study conducted in 2022. The settings of the study were 28 hospitals in Iran. The participants (n = 70) included staff from discharge, HIM, and IT departments. The data were collected using a researcher-made questionnaire and analyzed using descriptive and analytical statistics. The study results indicated that financial factors (2.36 ± 1.15) have the most effect on the execution of SEPAS. Also, users' workload (3.76 ± 1.19) had the most effect on the utilization rate. In addition, the results showed that there is a correlation between technical, user-related, organizational, financial, and managerial factors with utilization factors (P < 0.05). Also, the linear-regression results showed that technical, organizational, and financial factors had positive effects and aligned with utilization factors (P < 0.05). SEPAS, after the initial stages, is now on the growth path and development. For better execution of this system and to increase the utilization capacity, attention should be paid to the factors affecting the execution and utilization of the system. The factors raised in this study can be important for decision and policy-making in this area, especially for developing countries.
Subconjunctival injection of human umbilical cord mesenchymal stem cells alleviates experimental allergic conjunctivitis via regulating T cell response
Authors: Li Dongli , Ou Qingjian , Shen Qi , Lu Michael Mingze , Xu Jing-Ying , Jin Caixia , Gao Furong , Wang Juan , Zhang Jingfa , Zhang Jieping , Li Jiao , Lu Lixia , Xu Guo-Tong , Tian Haibin 
Published: June 2023

Abstract
Background
T helper 2 (Th2) cells are thought to play critical roles in allergic conjunctivitis (AC). They release inflammatory cytokines to promote an allergic response in AC. Due to individual heterogeneity and long-term chronic management, current therapies do not always effectively control AC. Mesenchymal stem cells (MSCs) have been shown to be effective in treating allergy-related disorders, but it is unclear how exactly the Th2-mediated allergic response is attenuated. This study aims to elucidate the therapeutic effect and mechanism of the human umbilical cord MSCs (hUCMSCs) in a mouse model of experimental AC (EAC).
Methods
A mouse EAC model was established by inoculating short ragweed (SRW) pollen. After the SRW pollen challenge, the mice received a single subconjunctival or tail vein injection of 2 x 106 hUCMSCs; subsequent scratching behavior and clinical symptoms were assessed. Immunostaining and flow cytometry were carried out to show allergic reactions and the activation of CD4 + T cell subsets in the conjunctiva and cervical lymph nodes (CLNs). Gene expression was determined by RNA-seq and further verified by qRT-PCR and Western blot. Co-culture assays were performed to explore the regulatory role of hUCMSCs in the differentiation of CD4 + Th2 cells.
Results
Subconjunctival administration of hUCMSCs resulted in fewer instances of scratching and lower inflammation scores in EAC mice compared to the tail-vein delivery and control groups. Subconjunctival administration of hUCMSCs reduced the number of activated mast cells and infiltrated eosinophils in the conjunctiva, as well as decreased the number of Th2 cells in CLNs. After pretreatment with EAC mouse serum in vitro to mimic the in vivo milieu, hUCMSCs were able to inhibit the differentiation of naive T cells (Th0) into Th2 cells. Further evidence demonstrated that repression of Th2 cell differentiation by hUCMSCs is mediated by CRSPLD2 through downregulation of STAT6 phosphorylation. Additionally, hUMCSCs were able to promote the differentiation of Th0 cells into regulatory T cells (Treg) in CLNs of EAC mice.
Conclusions
Subconjunctival injection of hUCMSCs suppressed the Th2-allergic response and alleviated clinical symptoms. This study provides not only a potential therapeutic target for the treatment of AC but also other T cell-mediated diseases.
Knowledge, Attitude, and Concerns related to Next Generation Sequencing in General Population in India: A Pilot Study
Authors: Phadke Shubha , Shambhavi Arya , Moirangthem Amita , Mishra Prabhaker 
Published: June 2023

Abstract
Next Generation Sequencing (NGS) technology is being widely used for preconception carrier screening of couples with or without family history of a genetic disorder, newborn screening, and for personalized and preventive treatments although its knowledge is still limited in general population and health care workers. In this pilot study, we have assessed the knowledge, attitude and concerns related to next generation sequencing in the general population in India. The present prospective cross-sectional survey was performed between June 2022 and September 2022. The predesigned semi structures questionnaire was used to collect the data. A total of 103 apparently healthy individuals completed the questionnaires related to basic understanding assessment, personal utility, information sharing with family members, concerns surrounding NGS and positive impact. The participants in this study demonstrated relatively good general understanding of the information sheet (78% average correct response). We also observed that the majority wished to know the results of secondary findings for treatable conditions, untreatable conditions and also variants of uncertain significance. The public also wanted to sequence all the genes in their body, given a chance, and based on the results- carrier status, drug response, or susceptibility to various conditions, plan of action would be decided. They had a general willingness to share the results with their relatives. About half of them also wished to undergo prenatal testing based on the VUS result.
Exploring the effects of dietary inulin in rainbow trout fed a high-starch, 100% plant-based diet
Authors: Defaix Raphaël , Lokesh Jep , Frohn Laura , Bechec Mickael Le , Pigot Thierry , Véron Vincent , Surget Anne , Biasutti Sandra , Terrier Frédéric , Skiba-Cassy Sandrine , Roy Jérôme , Panserat Stéphane , Ricaud Karine Brugirard 
Published: June 2023

Abstract

Background
High dietary carbohydrates can spare protein in rainbow trout but may affect growth and health. Inulin, a prebiotic, could have nutritional and metabolic effects, along with anti-inflammatory properties in teleosts, improving growth and welfare. We tested this hypothesis in rainbow trout by feeding them a 100% plant-based diet, which is a viable alternative to fishmeal and fish oil in aquaculture feeds. In a two-factorial design, we examined the impact of inulin (2%) as well as the variation in the CHO/plant protein ratio on rainbow trout. We assessed the influence of these factors on zootechnical parameters, plasmatic metabolites, gut microbiota, production of Short-Chain Fatty Acid and lactic acid, as well as the expression of free-fatty acid receptors genes in the mid-intestine, intermediary liver metabolism, and immune markers.
Results
The use of 2% inulin did not change significantly the fish intestinal microbiota, while interestingly, the high CHO/Protein ratio group shows modification of intestinal microbiota and in particular the beta diversity, with 21 bacterial genera affected, including Ralstonia, Bacillus, and 11 lactic-acid producing bacteria. There were higher levels of butyric, and valeric acid in groups fed with high CHO/protein diet but not with inulin. The high CHO/Protein group shows a decrease in the expression of pro-inflammatory cytokines (il1b, il8, tnfa) in liver and a lower expression of the genes coding for tight-junction proteins in mid-intestine (tjp1a, tjp3). However, the 2% inulin did not modify the expression of plasma immune markers. Finally, inulin induced a negative effect on rainbow trout growth performance irrespective of the dietary carbohydrates.
Conclusions
with a 100% plant-based diet, inclusion of high levels of carbohydrates could be a promising way for fish nutrition in aquaculture through a protein sparing effect whereas the supplementation of inulin in combination with such alternative diets needs further investigations.