bluuebunny/crossref_metadata_2025_split huggingface dataset:
Differential effects of WRAP53 transcript variants on non-small cell lung cancer cell behaviors
Authors: Zhu Yan , Sun Wenjie , Jiang Xueping , Bai Rui , Luo Yuan , Gao Yanping , Li Shuying , Huang Zhengrong , Gong Yan , Xie Conghua 
Published: January 2023

Background
The WD40-encoding RNA antisense to p53 (WRAP53) is an antisense gene of TP53 with three transcriptional start sites producing three transcript variants involved in the progression of non-small cell lung cancer. However, the mechanism by which these different transcript variants regulate non-small cell lung cancer cell behaviors is to be elucidated.

Methods
Two non-small cell lung cancer cell lines, A549 cells with wild-type p53 and H1975 with mutated p53, were transfected with WRAP53-1α and WRAP53-1β siRNA. The biological effects were assessed via colony formation, cell viability, apoptosis, cell cycle, wound healing and cell invasion assays, as well as immunoblotting.

Results
Knockdown of WRAP53-1α increased the mRNA and protein levels of p53; suppressed colony formation and proliferation of A549 cells but promoted them in H1975 cells; increased the proportion of cells in the G0/G1 phase in A549 cells but decreased that in H1975 cells; and suppressed migration and invasion in A549 cells but not in H1975 cells. Conversely, knockdown of WRAP53-1β had no effect on p53 expression; promoted the growth of A549 cells but not of H1975 cells; decreased the proportion of cells in the G0/G1 phase in A549 cells but not in H1975 cells; and promoted migration and invasion in A549 cells but not in H1975 cells. Knockdown of both WRAP53-1α and WRAP53-1β promoted apoptosis in A549 cells but not in H1975 cells.

Conclusions
WRAP53 transcript variants exerted different functions in non-small cell lung cancer cells and regulated non-small cell lung cancer cell behaviors depending on the p53 expression.
Reference intervals for thyroid biomarkers to enhance the assessment of thyroid status in childhood and adolescence
Authors: Ferraro Simona , Luconi Ester , Calcaterra Valeria , Cordaro Erika , Bianchi Alice , Cereda Cristina , Zuccotti Gianvincenzo , Kavsak Peter , Plebani Mario , Biganzoli Elia Mario , Marano Giuseppe , Boracchi Patrizia 
Published: January 2023

Abstract

Objectives
The determination of assay-dependent upper and lower reference limits (URL, LRL) of free triiodothyronine (FT3), free thyroxine (FT4), and thyroid stimulating hormone (TSH) during childhood and adolescence, is challenging.

Methods
Thyroid hormones were measured via the Abbott Alinity system in 502 euthyroid children partitioned in the following age groups: ≤2, 2.1–10, and 10.1–18 years. The 97.5th and 2.5th percentiles (URL and LRL) were derived according to CLSI EP28- A3c guidelines. Quantile regression models were used to assess: (a) 90% confidence intervals of the URL and LRL, (b) the effect of age on URL and LRL within each age class and on overall age range, (c) the difference between the URLs and LRLs estimated for each age partition with an estimate of the confidence interval divided by the reference interval being derived (CI/RI).

Results
The CI/RI for the LRLs are smaller as compared to the URLs, except for FT4 for the 2.1–10 years age group. Considering the CI/RI and the overlap between CIs across the three age groups, one single LRL might be considered for TSH, FT3 and FT4 between 0 and 18 years. However, for the URL, there was a noticeable decrease in the URL over the 3 age groups for all three biomarkers, with there being no overlap in CIs for the URL between the ≤2 vs. the 10.1–19 years age groups.

Conclusions
A common LRL for TSH, FT4 and FT3 for patients aged ≤18 years may be utilized when these biomarkers are measured with the Alinity system. For the URLs the use of age-specific URLs for these biomarkers is recommended.
On the global L
2-boundedness of Fourier integral operators with rough amplitude and phase functions
Authors: Sindayigaya Joachim 
Published: January 2023

Abstract
In this paper, we study the

L
2

{L^{2}}

-boundedness of Fourier integral operators

T

ϕ
,
a

{T_{\phi,a}}

with rough amplitude

a
⁢

(
x
,
η
)

{a(x,\eta)}

and rough phase functions

ϕ
⁢

(
x
,
η
)

{\phi(x,\eta)}

, which satisfies the new class of rough non-degeneracy condition. In this study, under the conditions

a
∈

L
∞

⁢

S
ρ
m

{a\in L^{\infty}S^{m}_{\rho}}

,

ϕ
∈

L
∞

⁢

Φ
2

{{\phi}\in L^{\infty}\Phi^{2}}

and some conditions of m, we show that

T

ϕ
,
a

{T_{\phi,a}}

is bounded from

L
2

{L^{2}}

to itself. Our main results extend and improve some known results about

L
2

{L^{2}}

, however, the method we use to prove these results is new.
B-essential spectra of 2 × 2 block operator matrix pencils
Authors: Abdmouleh Faiçal , Bahloul Aymen , Walha Ines 
Published: January 2023

Abstract
The paper is devoted to the study of the relative B-essential spectra of

2
×
2

{2\times 2}

unbounded operator matrix pencils defined with nondiagonal domain. The main tool used to prove the results is the Frobenuis–Schur factorization associated with this kind of operator matrix which has proven very useful in dealing with stability analysis in the theory of B-Fredholm operators pencils. Therefore, the theoretical results are tested in pencil transport integro-differential equation with specific boundary conditions.
Influence of mold cavity thickness on electrical, morphological and thermal properties of polypropylene/carbon micromoldings
Authors: Zhou Shengtai , Jiang Renze , Lei Xue , Zou Huawei , Hrymak Andrew N. 
Published: January 2023

Abstract
In this work, a comparative study on the electrical conductivity (σ) and thermal properties of polypropylene (PP)/carbon microparts with different part thickness (namely, 0.85 and 0.50 mm) is reported. Two different types of carbon filler (i.e., CNT and CB) were adopted to study the efficacy of different carbon fillers in improving the σ of PP/carbon microparts. In general, the σ of 0.85 mm thickness microparts were higher than the 0.50 mm thickness microparts, regardless of the carbon filler type and testing directions. This suggested that higher shearing conditions that prevailed in the microinjection molding (μIM) process were unfavorable for the formation of intact conductive pathways in corresponding moldings, albeit the distribution of carbon fillers turned better with increasing shear rates, as confirmed by morphology observations. Differential scanning calorimetry results showed that prior thermomechanical histories (including melt blending and μIM) experienced by the polymer melts had an influence on the thermal behavior of subsequent moldings. Also, there existed a strong shear flow-induced crystallization of polymer chains during μIM because the crystallinity of microparts was higher than that of feed materials.
Fabrication-conscious neural network based inverse design of single-material variable-index multilayer films
Authors: Yesilyurt Omer , Peana Samuel , Mkhitaryan Vahagn , Pagadala Karthik , Shalaev Vladimir M. , Kildishev Alexander V. , Boltasseva Alexandra 
Published: January 2023

Abstract
Multilayer films with continuously varying indices for each layer have attracted great deal of attention due to their superior optical, mechanical, and thermal properties. However, difficulties in fabrication have limited their application and study in scientific literature compared to multilayer films with fixed index layers. In this work we propose a neural network based inverse design technique enabled by a differentiable analytical solver for realistic design and fabrication of single material variable-index multilayer films. This approach generates multilayer films with excellent performance under ideal conditions. We furthermore address the issue of how to translate these ideal designs into practical useful devices which will naturally suffer from growth imperfections. By integrating simulated systematic and random errors just as a deposition tool would into the optimization process, we demonstrated that the same neural network that produced the ideal device can be retrained to produce designs compensating for systematic deposition errors. Furthermore, the proposed approach corrects for systematic errors even in the presence of random fabrication imperfections. The results outlined in this paper provide a practical and experimentally viable approach for the design of single material multilayer film stacks for an extremely wide variety of practical applications with high performance.
Infancy‐onset diabetes caused by de‐regulated AMPylation of the human endoplasmic reticulum chaperone BiP
Authors: Perera Luke A , Hattersley Andrew T , Harding Heather P , Wakeling Matthew N , Flanagan Sarah E , Mohsina Ibrahim , Raza Jamal , Gardham Alice , Ron David , De Franco Elisa 
Published: January 2023

AbstractDysfunction of the endoplasmic reticulum (ER) in insulin‐producing beta cells results in cell loss and diabetes mellitus. Here we report on five individuals from three different consanguineous families with infancy‐onset diabetes mellitus and severe neurodevelopmental delay caused by a homozygous p.(Arg371Ser) mutation in FICD. The FICD gene encodes a bifunctional Fic domain‐containing enzyme that regulates the ER Hsp70 chaperone, BiP, via catalysis of two antagonistic reactions: inhibitory AMPylation and stimulatory deAMPylation of BiP. Arg371 is a conserved residue in the Fic domain active site. The FICDR371S mutation partially compromises BiP AMPylation in vitro but eliminates all detectable deAMPylation activity. Overexpression of FICDR371S or knock‐in of the mutation at the FICD locus of stressed CHO cells results in inappropriately elevated levels of AMPylated BiP and compromised secretion. These findings, guided by human genetics, highlight the destructive consequences of de‐regulated BiP AMPylation and raise the prospect of tuning FICD's antagonistic activities towards therapeutic ends.
Entomofauna asociada a pasturas tropicales Cenchrus ciliaris, Chloris gayana y Megathyrsus maximus
Authors: Sierra-Barquero Paola Vanessa , Sánchez-Doria Tatiana , Burbano-Erazo Esteban 
Published: January 2023

Introduction. Pastures are the primary source of food in livestock systems and can be a suitable niche for insects with beneficial and harmful functions. Objective. To estimate the entomofauna associated with three species of pastures, Cenchrus ciliaris, Chloris gayana, and Megathyrsus maximus, in conditions of the Colombian Caribbean. Materials and methods. Ten samplings were carried out in two contrasting periods (August to October 2018 and January to March 2019), three forage species (Cenchrus ciliaris, Megathyrsus maximus, Chloris gayana) within 1000 m2 were used. Arthropods were identified by order and family and typified by functional groups. Diversity and abundance indices by pasture and season were estimated, as well as their correlation with climate. A simple correspondence analysis was performed. Results. A total of 380 insects from 7 orders and 35 families were collected, with the greatest abundance for Hemiptera and Coleoptera. The Shannon’s diversity index was higher for all orders of C. gayana, except for Hemiptera in C. ciliaris. Phytophages were found in a higher percentage (66.84 %), followed by predators (27.11 %). Insect abundance was influenced by the interaction between season and pasture, showing an increase at low temperatures, except for M. maximus. Pastures represented adequate microenvironments to maintain the diversity of insects, the Hemiptera and Coleoptera orders being the most numerous and representative of the functional groups of phytophages and predators, respectively. Conclusion. The entomofauna of C. gayana, C. ciliaris, and M. maximus was similar in insect abundance and diversity, however, insect abundance depended on the influence of the season and pastures.
Model fitting to concrete carbonation data with non-zero initial carbonation depth
Authors: Forsdyke Jessica C. , Lees Janet M. 
Published: January 2023

AbstractCarbonation is a key mechanism for deterioration of reinforced concrete structures. In design for resistance to carbonation, the performance of mixes is often specified and measured in laboratory carbonation tests where initial carbonation is sometimes present in specimens. The coherent interpretation of results from carbonation tests is vital as they enable a more accurate prediction of the performance of concrete structures in situ. This paper assesses two different approaches to considering the initial carbonation depth when extracting the carbonation coefficient from results of carbonation testing. Experimental data is compared to models for either approach using least squares regression. Both linear and non-linear representations of the initial carbonation depth are shown to fit the data well. The non-linear approach gives a larger estimate of carbonation coefficient than the linear approach, and is more consistent with the mathematical derivation of the carbonation equation. The ramifications of this difference will be most significant when the initial carbonation depth is large relative to the depth of the carbonation front. The accurate modelling of carbonation progression underpins performance-based design of new concrete structures and the assessment of existing concrete infrastructure.
Reactivity of C3S and model cement in presence of Na2S2O3 and NaSCN
Authors: Gonzalez-Panicello L. , De la Torre A. G. , Palacios M. 
Published: January 2023

AbstractThe impact of NaSCN and Na2S2O3 on the reactivity, microstructure and morphology of C3S and model cement (with a clinker containing 85% C3S and 15% C3A) pastes was systematically investigated. Results concluded that both alkali salts mainly act enhancing the reactivity of the C3S phase while not significant influence on the reactivity of C3A was measured. While both admixtures rose the reactivity of C3S over the studied 7 days of hydration, they only increased the reactivity of model cement pastes up to 14–20 h. NaSCN and Na2S2O3 did not modify the C–S–H stoichiometry but they influenced its morphology. In particular, thicker convergent C–S–H needles were formed in pastes containing Na2S2O3 compared to non-admixed systems, while a higher number of thinner C–S–H needles were formed in presence of NaSCN. Furthermore, greater portlandite clusters and intermixing of AFm and C–S–H were observed in admixed C3S and model cement pastes, respectively, compared to plain systems.
Temperature Distribution in Bio Stove using Saw Dust: An Integrated Project-based Learning
Authors: Wagiran W. , Mujiyono M. , Setiadi Bayu Rahmat , Wibowo Yulianto Eko , Surahmanto Fredy , Agata Dendy Aqila , Areeprasert Chinnathan 
Published: January 2023

This paper aims at developing the potential of cellulose-based energy generation using pyrolysis, gasification, and combustion methods with biodegradable waste media. The cellulose-based material used in this study was sawdust. The sawdust was heated using a biomass stove, which was then analyzed in terms of heat conduction and propagation as well as temperature distribution. To ensure the effectiveness of sawdust as the main material in the biomass stove, sawdust particles were pressed and compacted under various pressure conditions. This experiment was integrated with the Project-Based Learning method through the following steps: (1) determination of projects testing, (2) project design, (3) project implementation schedules, (4) project completion and progress monitoring, (5) reports and presentations of project results, and (6) project evaluation. The results provide new findings that the denser sawdust particles correlate with the greater temperature and propagation rate. This can be obtained from the measured temperature distribution. Areas close to the heat source tend to have the same heat propagation. The density of the sawdust particle is the main key point for producing better pyrolysis and gasification process, in which it correlates with long combustion energy. Integrasi This finding opens a new concept and can be used as a reference for other researchers who develop research related to renewable energy from waste, especially when using a biomass stove. This study also gives ideas for the need for developing project-based learning using the burning of sawdust using biomass stove as a tool for the teaching and learning process.
“If you can’t be a whole person, it’s hard to be a good doctor.” An Interview with Duke OB/GYN Residency Director Dr. Beverly Gray
Authors: Ugolik Phillips Kaitlin 
Published: January 2023

In the wake of COVID-19 and the overturn of Roe v Wade, medical residents—in particular OB/GYNs—are advocating for more family-friendly workplaces and reproductive choice. In this interview, Managing Editor Kaitlin Ugolik Phillips talks with Duke OB/GYN Residency Director Dr. Beverly Gray about the need for policy changes in medical education to improve workforce well-being and cultural competence.
Philanthropy Profile: A Conversation with Elizabeth Star-Winer of the Winer Family Foundation
Authors: Ugolik Phillips Kaitlin 
Published: January 2023

As a Lactation Consultant and child birth educator with Novant Health in Charlotte, Elizabeth Star-Winer worked with hundreds of families, both in a hospital setting and in the community. Those experiences instilled in her a desire to focus her philanthropy on addressing the root causes of the challenges and disparities she observed, particularly the high rates of maternal and infant mortality across the state.1 “Because of my hands-on career experiences, I wanted our foundation to focus on improving outcomes in two areas: maternal and infant health and creating awareness of the long-term effects of trauma and toxic stress on families,” Star-Winer told the NCMJ. “We are intentional on focusing our work upstream with the understanding that systems change takes time and investment in cross-sector partnerships and strong leadership.” NCMJ Managing Editor Kaitlin Ugolik Phillips spoke with Star-Winer about how she transitioned from working directly with families to running her family’s foundation with a mission of building capacity and aligning systems to ensure all families with children, from prenatal to age five, thrive across our state.
Cost-effectiveness of Strategies for Postpartum Glucose Intolerance Testing
Authors: Acosta Tatiana , Myers Evan , McElligott Kara 
Published: January 2023

Background Abnormal glucose metabolism after gestational diabetes mellitus can persist postpartum. This study compares the cost and effectiveness of 4 postpartum glucose intolerance screening strategies and their influencing variables. Methods A decision model compared 4 screening strategies: Fasting capillary glucose test (FCG) 24–72 hours postpartum (inpatient FCG) alone (strategy 1); Inpatient FCG plus 2-hour oral glucose tolerance test (OGTT) (strategy 2) or plus home FCG testing (strategy 3) at 4–12 weeks postpartum; No screening (strategy 4). Clinical probabilities and costs were obtained from institutional data, the literature, retail prices, and reimbursement data. The primary outcome was the per-patient cost and effectiveness of detecting diabetes and prediabetes (i.e., disease) and the incremental cost-effectiveness ratio (ICER) between strategies. Effectiveness was the proportion of patients with disease detected. Sensitivity analyses examined parameter uncertainty and variance on primary outcome. Results The base case cost and effectiveness per strategy ranged from $1167 to $1330 and 0%–25%, respectively. Strategy 3 was the most effective and expensive strategy compared to strategy 2 (ICER $837), and remained so when < 36% of patients completed the 2-hour OGTT and when the sensitivity of home FCG testing for detecting diabetes was > 39%. Strategy 2 was more effective and expensive than strategy 1 (ICER $571). Limitations Our model underestimates the cost of a missed case by limiting the horizon to 1 year because of the available data. This is appropriate for our study perspective, but future study is needed to estimate the effect of a longer time horizon on model outcomes. Conclusion Home FCG testing offers a reasonable alternative to the 2-hour OGTT when adherence to the 2-hour OGTT is low. This model serves as a tool to guide postpartum glucose intolerance screening protocols, minimizing missed opportunities for diagnosis and early intervention.
PENGARUH IDE PEMBAHARUAN ABDUH DI MESIR PADA TRADISI TAFSIR DI INDONESIA: KAJIAN TERHADAP TAFSIR QUR’AN KARIM KARYA MAHMUD YUNUS
Authors: Anwar Rosihon , Muhyi Asep Abdul , Riyani Irma 
Published: January 2023

Qur’anic Interpretation in Indonesia has been long interconnected with the tradition in the Middle East, especially in Haramayn and Egypt. Many Indonesians studied many Islamic disciplines from prominent ulama in these regions. One of them is Mahmud Yunus, who studied at Al-Azhar University, Cairo. He was influenced by the prominent ulama of Al- Azhar, i.e., M. Abduh. Abduh is famous for his thought of Islamic revivalism. This research investigates the possible influence of Abduh’s reformation thought to Mahmud Yunus Qur’anic interpretation, which called tafsir Qur’an Karim. This research employs a qualitative method with a historical approach and content analysis to analyze the data. This research shows that Mahmud Yunus is influenced significantly by Abduh’s reformation thought and can be seen from Yunus’ interpretation in his tafsir. The influence includes combating blind taqlid (unquestioning acceptance of religious thoughts), rationalizing the ‘mythical’ understanding of the Qur’anic texts, and emphasizing the belief that the Qur’an is compatible with science and the changing social reality. Kajian tafsir di Indonesia memiliki keterkaitan yang erat dengan pemikiran tafsir di Timur Tengah. Hal ini dapat dipahami karena banyak ulama Indonesia belajar di sana terutama Haramayn dan Mesir. Salah satu ulama yang memiliki keterpengaruhan tersebut adalah Mahmud Yunus yang sempat belajar di Al-Azhar, Kairo, Mesir. Secara spesifik, Yunus banyak mengadopsi pemikiran pembaharuan dari tokohpembaharu Mesir yakni M. Abduh. Penelitian ini bermaksud menelusuri keterpengaruhan pemikiran pembaharuan Mahmud Yunus dalam karya Tafsirnya Qur’an Karim dari pemikiran pembaharuan M. Abduh. Penelitian ini menggunakan metode kualitatif dengan menggali kajian pustakaatas naskah tafsir Mahmud Yunus dan Al-Manar dengan menggunakan analisi isi dan historis. Penelitian ini menunjukkan bahwa pengaruh pembaharuan Abduh terlihat dalam penafsiran yang dilakukan oleh Mahmud Yunus dalam Tafsirnya berkaitan dengan beberapa aspek diantaranya adalah: penolakan atas taqlid, bid’ah dan khurafat, rasionalitas atas beberapa pemahaman yang bersifat mitis dan kesesuaian antara al-Qur’an dengan perkembangan ilmu pengetahuan dan perkembangan masyarakat.