bluuebunny/crossref_metadata_2025_split huggingface dataset:
Molecular physiology of Antarctic diatom natural assemblages reveals multiple strategies contributing to their ecological success
Authors: Moreno Carly M. , Bernish Maggie , Meyer Meredith G. , Li Zuchuan , Waite Nicole , Cohen Natalie R. , Schofield Oscar , Marchetti Adrian 
Published: April 2023

AbstractThe continental shelf of the Western Antarctic Peninsula (WAP) is a highly variable system characterized by strong cross-shelf gradients, rapid regional change and large blooms of phytoplankton, notably diatoms. Rapid environmental changes coincide with shifts in plankton community composition and productivity, food web dynamics and biogeochemistry. Despite progress in identifying important environmental factors influencing plankton community composition in the WAP, the molecular basis for their survival in this oceanic region, as well as variations in species abundance, metabolism and distribution remain largely unresolved. Across a gradient of physicochemical parameters, we analyzed the metabolic profiles of phytoplankton as assessed through metatranscriptomic sequencing. Distinct phytoplankton communities and metabolisms closely mirrored the strong gradients in oceanographic parameters that existed from coastal to offshore regions. Diatoms were abundant in coastal, southern regions, where colder and fresher waters were conducive to a bloom of the centric diatom,Actinocyclus. Members of this genus invested heavily in growth and energy production; carbohydrate, amino acid and nucleotide biosynthesis pathways; and coping with oxidative stress, resulting in uniquely expressed metabolic profiles compared to other diatoms. We observed strong molecular evidence for iron limitation in shelf and slope regions of the WAP, where diatoms in these regions employed iron-starved induced proteins, a geranylgeranyl reductase, aquaporins, and urease, among other strategies, while limiting the use of iron-containing proteins. The metatranscriptomic survey performed here revealed functional differences in diatom communities and provides further insight into the environmental factors influencing the growth of diatoms and their predicted response to changes in ocean conditions.
Exploring the impact of read clustering thresholds on RADseq-based systematics: an empirical example from European amphibians
Authors: Rancilhac Loïs , Sylvestre Florent , Hutter Carl R. , Arntzen Jan W. , Babik Wieslaw , Crochet Pierre-Andre , Deso Grégory , Duguet Rémi , Galan Pedro , Pabijan Maciej , Policain Mathieu , Priol Pauline , Sabino-Pinto Joana , Capstick Maria , Elmer Kathryn R. , Dufresnes Christophe , Vences Miguel 
Published: April 2023

AbstractRestriction site-Associated DNA sequencing (RADseq) has great potential for genome-wide systematics studies of non-model organisms. However, accurately assembling RADseq reads into orthologous loci remains a major challenge in the absence of a reference genome. Traditional assembly pipelines cluster putative orthologous sequences based on a user-defined clustering threshold. Because improper clustering of orthologs is expected to affect results in downstream analyses, it is crucial to design pipelines for empirically optimizing the clustering threshold. While this issue has been largely discussed from a population genomics perspective, it remains understudied in the context of phylogenomics and coalescent species delimitation. To address this issue, we generated RADseq assemblies of representatives of the amphibian generaDiscoglossus, Rana, LissotritonandTriturususing a wide range of clustering thresholds. Particularly, we studied the effects of the intra-sample Clustering Threshold (iCT) and between-sample Clustering Threshold (bCT) separately, as both are expected to differ in multi-species data sets. The obtained assemblies were used for downstream inference of concatenation-based phylogenies, and multi-species coalescent species trees and species delimitation. The results were evaluated in the light of a reference genome-wide phylogeny calculated from newly generated Hybrid-Enrichment markers, as well as extensive background knowledge on the species’ systematics. Overall, our analyses show that the inferred topologies and their resolution are resilient to changes of the iCT and bCT, regardless of the analytical method employed. Except for some extreme clustering thresholds, all assemblies yielded identical, well-supported inter-species relationships that were mostly congruent with those inferred from the reference Hybrid-Enrichment data set. Similarly, coalescent species delimitation was consistent among similarity threshold values. However, we identified a strong effect of the bCT on the branch lengths of concatenation and species trees, with higher bCTs yielding trees with shorter branches, which might be a pitfall for downstream inferences of evolutionary rates. Our results suggest that the choice of assembly parameters for RADseq data in the context of shallow phylogenomics might be less challenging than previously thought. Finally, we propose a pipeline for empirical optimization of the iCT and bCT, implemented in optiRADCT, a series of scripts readily usable for future RADseq studies.
Derivation of trophoblast stem cells unveils unrestrained potential of mouse ESCs and epiblast
Authors: Jana Debabrata , Sailasree Purnima , Singh Priya , Srivastava Mansi , Vishnu Vijay V , Kale Hanuman T , Lakshmi Jyothi , Srinivas Gunda , Sowpati Divya Tej , Shekar P Chandra 
Published: April 2023

SUMMARYmESCs and epiblast are considered to follow strict lineage adherence and lack the potential to contribute to trophoectoderm. Here, we report the derivation of trophoblast stem cells (ESTS) from the mESCs. The single-cell transcriptome and molecular characterization of ESTS show similarity with TSCs. They efficiently integrate into the TE compartment of the blastocyst and contribute to the placenta during development. We discovered GSK3β as a critical regulator of the TE fate of ESCs. It plays a vital stage-specific role during ESTS derivation. We further show β-CATENIN and an intron-I regulatory element ofCdx2are essential for the TE fate of ESCs. We further show that the mouse epiblast can readily differentiate into TE lineage. In contrast to the paradigm of the restricted potential of pluripotent ESCs and epiblast, our data shows that murine ESCs and epiblast have the unrestrained developmental potential for extraembryonic lineages.
Multiple domains of the integral KREPA3 protein are critical for the structure and precise functions of RNA Editing Catalytic Complexes inTrypanosoma brucei
Authors: Davidge Brittney , McDermott Suzanne M , Carnes Jason , Lewis Isaac , Tracy Maxwell , Stuart Kenneth D. 
Published: April 2023

ABSTRACTThe gRNA directed U-insertion and deletion editing of mitochondrial mRNAs that is essential in different life cycle stages for the protozoan parasiteTrypanosoma bruceiis performed by three similar multi-protein catalytic complexes (CCs) that contain the requisite enzymes. These CCs also contain a common set of eight proteins that have no apparent direct catalytic function, including six that have an OB-fold domain. We show here that one of these OB-fold proteins, KREPA3 (A3), has structural homology to other editing proteins, is essential for editing and is multifunctional. We investigated A3 function by analyzing the effects of single amino acid loss of function mutations most of which were identified by screening bloodstream form (BF) parasites for loss of growth following random mutagenesis. Mutations in the ZFs, an intrinsically disordered region (IDR) and several within or near the C-terminal OB-fold domain variably impacted CC structural integrity and editing. Some mutations resulted in almost complete loss of CCs and its proteins and editing whereas others retained CCs but had aberrant editing. All but a mutation which is near the OB-fold affected growth and editing in BF but not procyclic form (PF) parasites. These data indicate that multiple positions within A3 have essential functions that contribute to the structural integrity of CCs, the precision of editing and the developmental differences in editing between BF and PF stages.
The interplay between the polar growth determinant DivIVA, the segregation protein ParA and their novel interaction partner PapM controls theMycobacterium smegmatiscell cycle by modulation of DivIVA subcellular distribution
Authors: Matusiak Izabela , Strzałka Agnieszka , Wadach Patrycja , Szwajczak Ewa , Szydłowska-Helbrych Aleksandra , Kepplinger Bernhard , Pióro Monika , Jakimowicz Dagmara 
Published: April 2023

AbstractBacterial chromosome segregation is facilitated by the ParABS system. The ParB protein binds centromere-likeparSsequences and forms nucleoprotein complexes. These nucleoprotein complexes are segregated by the dynamic ATPase ParA. In mycobacteria, ParA also interacts with the polar growth determinant DivIVA (Wag31). This interaction was earlier shown to facilitate the segregation of ParB complexes but also to affect cell extension. Here, we identify an additional partner of ParA inMycobacterium smegmatis, named PapM. UsingE. colibased analysis, we show that PapM likewise interacts with DivIVA and that the tripartite interaction of ParA-PapM-DivIVA is phosphorylation-dependent: ParA binding to DivIVA is diminished, while PapM binding is promoted upon phosphorylation of DivIVA. The presence of PapM enhances the dissociation of ParA from the DivIVA complex upon its phosphorylation. Studies ofM. smegmatismutant strains reveal that altered PapM levels influence chromosome segregation and cell length. The elimination of PapM affects ParA dynamics. Further, ParA and, to a lesser extent, PapM modulates the subcellular distribution of DivIVA. Altogether our studies show that the tripartite interplay between ParA-DivIVA and PapM controls the switch between cell division and cell elongation and in this way affects the mycobacterial cell cycle.ImportanceThe genus of Mycobacteria includes important clinical pathogens (M. tuberculosis). Bacteria of this genus share the unusual features of their cell cycle such as asymmetric polar cell elongation and long generation time. Markedly, control of the mycobacterial cell cycle still remains not fully understood. The main cell growth determinant in Mycobacteria is an essential protein DivIVA, which is also involved in cell division. DivIVA activity is controlled by phosphorylation but the mechanism and significance of this process is unknown. Here, we show how the previously established protein interaction partner of DivIVA in Mycobacteria, the segregation protein ParA, affects the DivIVA subcellular distribution. We also demonstrate the role of a newly identifiedM. smegmatisDivIVA and ParA interaction partner, a protein named PapM, and we establish how their interactions are modulated by phosphorylation. Demonstrating that the tripartite interplay affects the mycobacterial cell cycle contributes to general understanding of mycobacterial growth regulation.
Mechanics reveals the role of peristome geometry in prey capture in carnivorous pitcher plants (Nepenthes)
Authors: Moulton Derek E. , Oliveri Hadrien , Goriely Alain , Thorogood Chris 
Published: April 2023

Carnivorous pitcher plants (Nepenthes) are a striking example of a natural pitfall trap. The trap’s slippery rim, or peristome, plays a critical role in insect capture via an aquaplaning mechanism that is well documented. Whilst the peristome has received significant research attention, the conspicuous variation in peristome geometry across the genus remains unexplored. We examined the mechanics of prey capture usingNepenthespitcher plants with divergent peristome geometries. Inspired by living materials, we developed a mathematical model that links the peristomes’ three-dimensional geometries to the physics of prey capture under the laws of Newtonian mechanics. Linking form and function enables us to test hypotheses related to the function of features such as shape and ornamentation, orientation in a gravitational field, and the presence of ‘teeth’, while analysis of the energetic costs and gains of a given geometry provides a means of inferring potential evolutionary pathways. In a separate modeling approach, we show how prey size may correlate with peristome dimensions for optimal capture. Our modeling framework provides a physical platform to understand how divergence in peristome morphology may have evolved in the genusNepenthesin response to shifts in prey diversity, availability, and size.Significance StatementPitcher plants (Nepenthes) produce an astonishing array of leaf-derived traps into which preys (typically insects) slide from a rim (the peristome). How prey capture varies across this varied genus is a mystery. We hypothesized that insects behave differently depending on peristome size and geometry. We examined the physics of prey capture under the laws of Newtonian mechanics to show that prey size and behavior correlate with peristome shape. We conclude that a diversity of peristomes inNepenthesevolved in response to variation in prey capture.
Rural youths' choice of livelihood strategies and their effect on income poverty and food security in Rwanda
Authors: Kangondo Angelique , Ndyetabula Daniel Wilson , Mdoe Ntengua , Mlay Gilead Isaac 
Published: April 2023

PurposeThis study aims at exploring the choices of livelihood strategies amongst the rural youth and how these choices relate to food security and income poverty.Design/methodology/approachThe study used data from the 2016/17 wave of Integrated Household Living Condition Survey, with a sample size of 1,050 rural youths. Statistical and econometrics methods including descriptive statistics and the Multinomial Endogenous Treatment Effect (METE) model were used to analyse the data.FindingsLivelihood choices were grouped into five categories, namely agriculture, non-farm wage employment, agriculture plus non-farm wage, agriculture plus self-employment and agriculture plus non-farm wage plus self-employment. The estimates from METE indicate that the youths' choice of non-farm wage, agriculture plus non-farm wage and agriculture plus self-employment contributes substantially to household food security improvement and poverty reduction. These findings show that agriculture is necessary but not a sufficient livelihood strategy to sustain the rural youth's contribution to youth's household welfare. The rural youth will pursue agriculture as a reliable source of livelihood not only for food self-sufficiency, but also for ensuring adequate return to labour.Originality/valueThis paper extends single choice analysis to multiple choices impact analysis, which has the advantage of accounting for selection bias due to both observed and unobserved heterogeneity. This paper assesses the differential impact of the choice of single as well as multiple livelihood strategies.
Source, message and medium? The role of personal values in forming credibility perceptions of non-sponsored product review videos
Authors: Alsaeed Ghadeer R. , Keeling Kathleen Anne , Sarantopoulos Panagiotis , Gadalla Eman 
Published: April 2023

Purpose
This paper aims to investigate an integrated, holistic assessment of the characteristics by which consumers judge non-sponsored product review video (PRV) source, message and medium components as credible, and how these are linked to personal values for a deeper understanding of multidimensional credibility assessments of PRVs.

Design/methodology/approach
Employing a means-end approach, the authors draw on credibility theory and the persuasion knowledge model to analyse data from 21 in-depth semi-structured laddering interviews.

Findings
First, the authors demonstrate distinctive contributions of the video modality towards PRV credibility assessments and the interplay between specific PRV characteristics, cognitive and socio-emotional consequences, and personal values in an ongoing process of credibility assessment. Second, high persuasion knowledge creates awareness of the potential phoniness of the market, revealing a dark side to PRV use even in non-sponsored PRV seemingly created and shared as an act of benevolent concern between consumers.

Research limitations/implications
This paper focused on the credibility of non-sponsored PRVs, future studies might investigate motivations and attributes by which users judge sponsored reviews. Also, the roles of specific product categories and existing brand trust on PRVs credibility provide avenues for further research.

Practical implications
This research offers practical implications for reviewers and brand managers to leverage the unique informational values of video by focusing on the interplay between credibility attributes and customer values.

Originality/value
This work advances credibility theory in the PRV context by examining how non-sponsored PRVs are evaluated as credible, by highlighting consumer persuasion knowledge and scepticism and including the holistic effects of the interplay between source, message and video format characteristics and by linking these to consumers’ goals and values.
Climate change adaptation based on computable general equilibrium models – a systematic review
Authors: Wei Taoyuan , Aaheim Asbjørn 
Published: April 2023

Purpose
This study aims to identify the current state of the art and the gaps in the application of computable general equilibrium (CGE) models on studying climate change adaptation.

Design/methodology/approach
A systematic review is conducted to select, classify and analyze relevant studies from two databases of Web of Science and Scopus.

Findings
Totally, 170 articles based on selected keywords were found from both databases, where 56 articles were duplicates. The authors further excluded 17 articles owing to preliminary exclusion criteria. Hence, 97 papers were selected for full-text review and more detailed assessment. Only a few of the studies explicitly have addressed the role of autonomous adaptation embodied in the CGE models. Over one-third of the studies have focused on planned adaptation without explicitly mentioning autonomous adaptation. Agriculture was the most addressed sector, and country-level models are the most adopted. Only one article has focused on South America.

Research limitations/implications
The review suggests that autonomous adaptation embodied in CGE models was not well addressed in the literature. As the limited studies have shown that autonomous adaptation can dramatically mitigate direct climate change impacts, further studies are needed to examine the importance of the autonomous adaptation for better understanding of climate change impacts. Furthermore, CGE models can provide a joint assessment considering both mitigation and adaptation strategies and management measures as such models have also been widely used to address effects of mitigation measures in the literature.

Originality/value
The studies on climate change adaptation based on CGE models have been systematically reviewed, and state-of-the-art knowledge and research gaps have been identified.
Do guests care more about hotel cleanliness during COVID-19? Understanding factors associated with cleanliness importance of hotel guests
Authors: Yang Yang , Lin Michael S. , Magnini Vincent P. 
Published: April 2023

Purpose
Growing health concerns amid the COVID-19 pandemic have led guests to focus on various aspects of hotel cleanliness. This study aims to investigate whether customers’ perceived importance of hotel cleanliness during their stay depends on local pandemic severity and moderators of the pandemic–cleanliness relationship.

Design/methodology/approach
Based on TripAdvisor data from 26,519 reviews in 2020 for 2,024 hotels across the USA, this study evaluated the importance of hotel cleanliness using the estimated coefficient of the cleanliness score in a regression of overall hotel rating scores.

Findings
Results of a multilevel ordered logit model confirmed that a more difficult local pandemic situation rendered cleanliness more important during hotel stays. Additionally, the effect of the pandemic was more pronounced among specific groups: men and travelers with more expertise, and guests staying in hotels without COVID-19 protocols for linen cleaning, with a lower average rating, with a larger size and in a more urbanized location.

Originality/value
This study represents a pioneering effort to assess how pandemics shape people’s (perceived) importance of cleanliness during hotel stays based on revealed data. Despite potential managerial relevance, a number of the moderating variables included in this study, such as traveler expertise and hotel location, have never been studied within the context of cleanliness perceptions during a pandemic.
Examining the cross-level mechanisms of the influence of supervisors’ job crafting on frontline employees’ engagement and performance
Authors: Zhao Xinyuan (Roy) , Wang Jiale , Mattila Anna , Leong Aliana Man Wai , Cui Zhenzhen , Sun Zaoning , Yang Chunjiang , Chen Yashuo 
Published: April 2023

Purpose
Frontline employees’ proactive behaviors (i.e. job crafting) are critical to satisfying customers’ idiosyncratic needs. If the supervisors practice job crafting, their subordinates are more likely to mimic such behaviors. However, there has been limited research on how leaders’ job crafting can influence subordinates’ job crafting. This study aims to examine the cross-level mechanisms (i.e. trickle-down effects) of supervisors’ job crafting on the subordinates’ attitudes and performance. Specifically, such trickle-down effects can be explained via two cross-level mechanisms of the supervisors’ job crafting on the subordinates’ work engagement and performance: social learning mechanism and job demands-resources mechanism.

Design/methodology/approach
A three-wave cross-lagged study was conducted in two-week intervals. The valid responses from 67 supervisors and their 201 subordinates were collected. The data set was analyzed using multilevel Structural Equation Modeling.

Findings
The results demonstrated that the social learning and job demands-resources mechanisms are not independent. The supervisor’s job crafting improves employment relationships, subsequently encourages subordinates’ job crafting and ultimately enhances work engagement and work performance.

Practical implications
The findings suggested that hospitality organizations should encourage job crafting among supervisors and managers. A proactive hotel manager can establish strong employment relationships, motivate subordinates to work proactively and obtain positive work outcomes.

Originality/value
The findings enrich the knowledge about the trickle-down effects of supervisors on subordinates in terms of job-crafting behaviors. In particular, this study found a new theoretical perspective that the job demands-resources and social learning mechanisms may not be independent, and the subordinates’ perception of the employment relationship plays a critical role.