bluuebunny/crossref_metadata_2025_split huggingface dataset:
Genetic Fusion of Thermoresponsive Polypeptides with UCST‐type Behavior Mediates 1D Assembly of Coiled‐Coil Bundlemers
Authors: Patkar Sai S. , Tang Yao , Bisram Arriana M. , Zhang Tianren , Saven Jeffery G. , Pochan Darrin J. , Kiick Kristi L. 
Published: March 2023

AbstractThermoresponsive resilin‐like polypeptides (RLPs) of various lengths were genetically fused to two different computationally designed coiled coil‐forming peptides with distinct thermal stability, to develop new strategies to assemble coiled coil peptides via temperature‐triggered phase separation of the RLP units. Their successful production in bacterial expression hosts was verified via gel electrophoresis, mass spectrometry, and amino acid analysis. Circular dichroism (CD) spectroscopy, ultraviolet‐visible (UV/Vis) turbidimetry, and dynamic light scattering (DLS) measurements confirmed the stability of the coiled coils and showed that the thermosensitive phase behavior of the RLPs was preserved in the genetically fused hybrid polypeptides. Cryogenic‐transmission electron microscopy and coarse‐grained modeling revealed that functionalizing the coiled coils with thermoresponsive RLPs leads to their thermally triggered noncovalent assembly into nanofibrillar assemblies.
Bicyclo[1.1.1]pentane Embedded in Porphyrinoids**
Authors: Grover Nitika , Cheveau Maxime , Twamley Brendan , Kingsbury Christopher J. , Mattern Cornelia M. , Senge Mathias O. 
Published: March 2023

AbstractWe report a two‐step approach to obtain synthetically versatile bicyclo[1.1.1]pentane (BCP) derivatives using Grignard reagents. This method allows the incorporation of BCP units in tetrapyrrolic macrocycles and the synthesis of a new class of calix[4]pyrrole analogues by replacing two bridging methylene groups with two BCP units. In addition, a doubly N‐confused system was also formed in the presence of electron‐withdrawing substituents at the BCP bridgeheads. The pyrrole rings in BCP containing macrocycles exist in 1,3‐alternate or αβαβ conformations, as observed from single‐crystal X‐ray diffraction analyses and 2D NMR spectroscopy.
Solid‐State Luminescence Stimulus‐Responsive Property and Doped RTP Emission of Novel 7a,8,10,11‐Tetrahydro‐9H‐ pyrido[2′,1′:2,3][1,3]oxazino[6,5,4‐ij]isoquinoline Derivative
Authors: Wang Yuzhe , Liu Miaochang , Zhang Weijing , Lei Yunxiang , Zhou Yunbing , Gao Wenxia , Ding Qiuping , Wu Huayue , Huang Xiaobo 
Published: March 2023

AbstractA novel 7a,8,10,11‐tetrahydro‐9H‐pyrido[2′,1′:2,3][1,3]‐oxazino[6,5,4‐ij]isoquinoline derivative (POIQ) is accidentally obtained from an isoquinoline derivative and iodine in dimethyl sulfoxide, which is demonstrated to undergo a mechanism of demethylation and thus intramolecular nucleophilic substitution. POIQ with twisted molecular conformation and loose stacking arrangement shows multifunctional optical properties including dual‐state emission, mechanochromic, and solid‐state acidochromic activities. Furthermore, an organic room temperature phosphorescence (RTP) doped system with green afterglow of 2 s is constructed using POIQ as the guest and easily available phenyl benzoate as the host, in which the host molecules assist in the transfer of the triplet excitons of the guest molecules. Rewritable optical recording media and information encryption are also realized based on multifunctional optical properties of this compound. This work provides inspiration for the development of N,O‐containing fused‐ring compounds with fluorescence/RTP properties.
Two‐channel false data injection attacks on multi‐sensor remote state estimation
Authors: Hua Jinxing , Liu Ruirui , Hao Fei 
Published: March 2023

AbstractThis paper investigates a security problem in cyber‐physical systems (CPSs) under multisensory framework. In the considered system, the feedback and feed‐forward channels may suffer from false data injection (FDI) attacks. The attacker aims at disturbing the systems by modifying the sensors' measurements and injecting external control inputs while keep (
)‐stealthiness. Two types of multi‐sensor fusion Kalman filters, which are equivalent in the absence of attacks, are adopted by the remote state estimator. When the system is maliciously attacked, the necessary and sufficient condition is obtained to guarantee the equivalence of the sequential Kalman filter and data compression Kalman filter under two‐channel attacks. Moreover, suboptimal (
)‐stealthiness attack strategies for these Kalman filters are obtained by applying two‐stage optimization method, convex analysis and monotonic optimization techniques. Finally, simulations on a numerical example of a quadrotor are carried out to validate the feasibility of theoretical results.
A CHO stable pool production platform for rapid clinical development of trimeric SARS‐CoV‐2 spike subunit vaccine antigens
Authors: Joubert Simon , Stuible Matthew , Lord‐Dufour Simon , Lamoureux Linda , Vaillancourt François , Perret Sylvie , Ouimet Manon , Pelletier Alex , Bisson Louis , Mahimkar Rohan , Pham Phuong Lan , L′Ecuyer‐Coelho Helene , Roy Marjolaine , Voyer Robert , Baardsnes Jason , Sauvageau Janelle , St‐Michael Frank , Robotham Anna , Kelly John , Acel Andrea , Schrag Joseph D. , El Bakkouri Majida , Durocher Yves 
Published: March 2023

AbstractProtein expression from stably transfected Chinese hamster ovary (CHO) clones is an established but time‐consuming method for manufacturing therapeutic recombinant proteins. The use of faster, alternative approaches, such as non‐clonal stable pools, has been restricted due to lower productivity and longstanding regulatory guidelines. Recently, the performance of stable pools has improved dramatically, making them a viable option for quickly producing drug substance for GLP‐toxicology and early‐phase clinical trials in scenarios such as pandemics that demand rapid production timelines. Compared to stable CHO clones which can take several months to generate and characterize, stable pool development can be completed in only a few weeks. Here, we compared the productivity and product quality of trimeric SARS‐CoV‐2 spike protein ectodomains produced from stable CHO pools or clones. Using a set of biophysical and biochemical assays we show that product quality is very similar and that CHO pools demonstrate sufficient productivity to generate vaccine candidates for early clinical trials. Based on these data, we propose that regulatory guidelines should be updated to permit production of early clinical trial material from CHO pools to enable more rapid and cost‐effective clinical evaluation of potentially life‐saving vaccines.
Thirteen‐year trends in risk scores predictive values for subsequent stroke in patients with acute ischemic event
Authors: Xiong Yunyun , Wang Shang , Li Zixiao , Fisher Marc , Wang Liyuan , Jiang Yong , Huang Xinying , Zhao Xing‐Quan , Meng Xia , Wang Yongjun 
Published: March 2023

AbstractIntroductionA high residual risk of subsequent stroke suggested that the predictive ability of Stroke Prognosis Instrument‐II (SPI‐II) and Essen Stroke Risk Score (ESRS) may have changed over the years.AimTo explore the predictive values of the SPI‐II and ESRS for 1‐year subsequent stroke risk in a pooled analysis of three consecutive national cohorts in China over 13 years.ResultsIn the China National Stroke Registries (CNSRs), 10.7% (5297/50,374) of the patients had a subsequent stroke within 1 year; area under the curve (AUC) of SPI‐II and ESRS was .60 (95% confidence interval [CI]: .59–.61) and .58 (95% CI: .57–.59), respectively. For SPI‐II, the AUC was .60 (95% CI: .59–.62) in CNSR‐I, .60 (95% CI: .59–.62) in CNSR‐II, and .58 (95% CI: .56–.59) in CNSR‐III over the past 13 years. The declining trend was also found in ESRS scale (CNSR‐I: .60 [95% CI: .59–.61]; CNSR‐II: .60 [95% CI: .59–.62]; and CNSR‐III: .56 [95% CI: .55–.58]).ConclusionsThe predictive power of the traditional risk scores SPI‐II and ESRS was limited and gradually decreased over the past 13 years, thus the scales may not be useful for current clinical practice. Further derivation of risk scales with additional imaging features and biomarkers may be warranted.
Structural alterations of the salience network in patients with insular glioma
Authors: Hu Guanjie , Wu Zhiqiang , Cao Bowen , Shi Qinyu , Zhang Zifeng , Fan Xiao , Tang Yao , Cheng Zhangchun , Wang Xiefeng , Jing Shenqi , Li Tao , Zhang Junxia , You Yongping 
Published: March 2023

AbstractObjectiveThe structural alteration that occurs within the salience network (SN) in patients with insular glioma is unclear. Therefore, we aimed to investigate the changes in the topological network and brain structure alterations within the SN in patients with insular glioma.MethodsWe enrolled 46 patients with left insular glioma, 39 patients with right insular glioma, and 21 demographically matched healthy controls (HCs). We compared the topological network, gray matter (GM) volume, and fractional anisotropy (FA) between HCs and patients after controlling for the effects of age and gender.ResultsPatients with insular glioma showed topological network decline mainly in the insula, basal ganglia region, and anterior cingulate cortex (ACC). Compared with HCs, patients primarily showed GM volume increased in the ACC, inferior temporal gyrus (ITG), superior temporal gyrus (STG), temporal pole: middle temporal gyrus (TPOmid), insula, middle temporal gyrus (MTG), middle frontal gyrus, and superior occipital gyrus (SOG), but decreased in TPOmid, ITG, temporal pole: superior temporal gyrus, and SOG. FA declined mainly in the STG, MTG, ACC, superior frontal gyrus, and SOG, and also showed an increased cluster in SOG.ConclusionsFA represents the integrity of the white matter. In patients with insular glioma, decreased FA may lead to the destruction of the topological network within the SN, which in turn may lead to the decrease of network efficiency and brain function, and the increase of GM volume may compensate for these changes. Overall, this pattern of structural changes provides new insight into the compensation model of insular glioma.
JNK‐IN‐8 treatment improves ARDS‐induced cognitive impairment by inhibiting JNK/NF‐κB‐mediated NLRP3 inflammasome
Authors: Shi Yunchao , Fang Ying , Shen Peng , Liu He , Xu Longsheng , Wang Liyan , Yang Maoxian 
Published: March 2023

AbstractPurposeCognitive impairment is a critical complication of acute respiratory distress syndrome (ARDS). However, effective interventions are lacking. Growing evidence demonstrates that c‐Jun N‐terminal kinase (JNK)‐mediated neuroinflammation is involved in the development of ARDS. Therefore, we hypothesized that the JNK pathway is involved in ARDS‐induced cognitive impairment.MethodsAn in vivo rat model of ARDS was established by treating it with lipopolysaccharide. The cognitive function was assessed by behavioral tests. The levels of pro‐inflammatory cytokines, JNK and NOD‐, LRR‐, and pyrin domain‐containing protein 3 (NLRP3) were analyzed by enzyme‐linked immunosorbent assay, western blot, or immunohistochemical analysis.ResultsWe found that JNK inhibitor 8 (JNK‐IN‐8) alleviated cognitive impairment, neuroinflammation, and NLRP3 inflammasome activation in the ARDS rat model. Additionally, an in vivo study showed that the protective effect of JNK‐IN‐8 on cognitive impairment was blocked by nigericin, an NLRP3 activator.ConclusionsOur data suggest that JNK‐IN‐8 treatment improves ARDS‐induced cognitive impairment by inhibiting the JNK/nuclear factor‐κB‐mediated NLRP3 inflammasome.
How Does Brazilian Green Propolis Act in Normal and Tumor Cells? Identification of Compounds in or out Monocytes or HEp‐2 Cells
Authors: Farlow Anthony , José Crevelin Eduardo , Basso Santiago Karina , de Oliveira Cardoso Eliza , Matsumoto da Silva Livia , Kenupp Bastos Jairo , Maurício Sforcin José 
Published: March 2023

AbstractThe aim of this study was to identify propolis compounds after incubation of normal and tumor cells (monocytes and HEp‐2 cells, respectively) with Brazilian green propolis, in the lysate and supernatant of cell cultures and within these cells by gas chromatography‐mass spectrometry (GC/MS). Cinnamic acid derivatives were generally localized in the lysate of both cell lines after incubation, suggesting these compounds are actively transported across the membrane into the cytoplasm. Terpenes were also found in the lysate. Artepillin C, in contrast, was localised only in the supernatant. Some constituents were unobservable after incubation, especially in monocytes, suggesting the compounds had been degraded. Our findings shed light on the possible sites of action (intracellular or via a cell membrane protein) and the bioavailability of various constituents of propolis, as well as possible modes of delivery of bioactive constituents.
Deconstructing the Potency and Cell‐Line Selectivity of Membranolytic Anticancer Peptides**
Authors: Martinez‐Hernandez Cristina , del Carmen Aguilera‐Puga Mariana , Plisson Fabien 
Published: March 2023

AbstractCurrent cancer treatments damage healthy cells and tissues, causing short‐term and long‐term side effects. New treatments are desired that show greater selectivity toward cancer cells and evade the common mechanisms of multidrug resistance. Membranolytic anticancer peptides (mACPs) hold promise against cancer and multidrug resistance. Amphipathicity, hydrophobicity, and net charge of mACPs participate in their respective interactions with cell membranes and their overall inhibition of cancer cells. To support the design of cell‐line selective mACPs, we investigated the relationships that amino acid composition, physicochemical properties, sequence motifs, and sequence homology could have with their potency and selectivity towards several healthy and cancer cell lines. Sequence length and net charge are known to affect the selectivity of mACPs between cancer and healthy cell lines. Our study reveals that increasing the net charge or flexibility (i. e., small and aliphatic residues) influences their selectivity between cancer cell lines with comparable lipid compositions.
Bufalin induces apoptosis and autophagy via the Ca2+/CaMKKβ/AMPK/Beclin1 signaling pathway in osteosarcoma cells
Authors: Zheng Ya‐Dong , He Zhen , Su Zhu‐Cheng , Wang Hui , Jiang Xiao‐Hong , Fang Xu , Lu Sheng‐Lin , Li Yuan 
Published: March 2023

AbstractBufalin, a major cardiotonic compound of the traditional Chinese medicine Chanshu has been used for cancer treatment for several years. However, the molecular mechanisms of Bufalin‐induced autophagy in osteosarcoma (OS) is not fully understood. In the present study, it was shown that Bufalin induced crosstalk between apoptosis and autophagy, which resulted in OS cell death. Mechanistically, Bufalin induced autophagy by increased the ratio of microtubule‐associated protein 1A/1B‐light chain 3 (LC3)‐II/LC3‐I, and inducing apoptosis via the caspase‐dependent pathway. Inhibition of autophagy promoted Bufalin‐induced cell death. In contrast, suppression of apoptosis enhanced Bufalin‐induced autophagy. In addition, it was found that Bufalin activated the Ca2+/calmodulin‐dependent protein kinase β/AMPK/Beclin1 pathway, which resulted in induction of autophagy. These findings provide a mechanistic understanding of the means by which Bufalin mediates autophagy and apoptosis in OS cells.
Selective, Aerobic Oxidation of Methane to Formaldehyde over Platinum ‐ a Perspective
Authors: van Steen Eric , Guo Junfeng , Hytoolakhan Lal Mahomed Nasseela , Leteba Gerard M. , Mahlaba Sinqobile V. L. 
Published: March 2023

AbstractThe direct formation of formaldehyde in the selective, aerobic oxidation of methane in the presence of liquid water at ca. 498 K has recently been described. One of the intriguing observations in the aerobic oxidation of methane over platinum in the presence of water is the absence of the typically observed negative selectivity‐conversion dependency (up to a methane conversion of 1 %). In this perspective, the main differences between this route and other methods for the selective oxidation of methane are being described. Possible reaction pathways for the formation of formaldehyde are discussed. Further developments for this newly developed methane activation route are highlighted.
Differential Evolution‐Based Optimization in Dimethyl Oxalate Hydrogenation
Authors: Rohman Fakhrony Sholahudin , Muhammad Dinie , Zahan Khairul Azly , Aziz Norashid , Murat Muhamad Nazri 
Published: March 2023

AbstractMulti‐objective optimization of dimethyl oxalate (DMO) hydrogenation to produce ethylene glycol (EG) is performed for three problems with four different objectives: maximization of DMO conversion and minimization of energy cost for problem 1; maximization of productivity and minimization of energy cost for problem 2; maximization of productivity and minimization of side product for problem 3; minimization of bare module and energy cost for problem 4; maximization of productivity and minimization of bare module for problem 5, and maximization of productivity and minimization of bare module and energy cost for problem 6. Multi‐objective differential evolution with ranking‐based mutation operator is applied to tackle the optimization problem with ASPEN simulator as model‐based optimization for EG production. The plots of decision variables denote that the optimal solution is strongly dependent on temperature.
Trumpet‐Like ZnS@C Composite for High‐Performance Potassium Ion Battery Anode
Authors: Gan Yunfei , Mu Meiqi , Li Mingquan , Ma Xiangdong , Yuan Jujun , He Haishan , Li Xiaokang , Mou Jirong , Zhang Chao , Zhang Xianke , Liu Jun 
Published: March 2023

AbstractZnS has acquired increasing attention for high‐performance PIBs anode because of its remarkable theoretical capacity, and redox reversibility for conversion reaction. However, the larger volume variation and delayed reaction kinetics for the ZnS in the discharge/charge processes lead to pulverization and severe capacity degradation. Herein, the trumpet‐like ZnS@C composite was synthesized by template method by using sodium citrate as carbon source followed by vulcanization process. As potassium ion batteries (PIB) anode, ZnS@C composite exhibits good rate performance and long life (stable reversible capacity of 107.8 mAh/g over 2000 charge‐discharge cycles at 5 A/g and high reversible capacity of 310 mAh/g at 0.1 A/g). The outstanding electrochemical performance of the ZnS@C composite is ascribed to its unique structure, which can mitigate the volume expansion of ZnS in the charge discharge process, expand the contact area between the electrode and electrolyte, and improve the conductivity of electrode materials by the introduction of carbon layer. This method of synthesizing trumpet‐like ZnS@C composite provides an important strategy for obtaining potassium ion batteries anode with long cycle.
Redox Reporter ‐ Ligand Competition to Support Signaling in the Cocaine‐Binding Electrochemical Aptamer‐Based Biosensor
Authors: Dauphin‐Ducharme Philippe , Churcher Zachary R. , Shoara Aron A. , Rahbarimehr Erfan , Slavkovic Sladjana , Fontaine Nicolas , Boisvert Olivia , Johnson Philip E. 
Published: March 2023

AbstractElectrochemical aptamer‐based (E‐AB) biosensors have demonstrated capabilities in monitoring molecules directly in undiluted complex matrices and in the body with the hopes of addressing personalized medicine challenges. This sensing platform relies on an electrode‐bound, redox‐reporter‐modified aptamer. The electrochemical signal is thought to originate from the aptamer undergoing a binding‐induced conformational change capable of moving the redox reporter closer to the electrode surface. While this is the generally accepted mechanism, it is notable that there is limited evidence demonstrating conformational change or distance‐dependent change in electron transfer rates in E‐AB sensors. In response, we investigate here the signal transduction of the well‐studied cocaine‐binding aptamer with different analytical methods and found that this sensor relies on a redox‐reporter ‐ ligand competition mechanism rather than a ligand‐induced structure formation mechanism. Our results show that the covalently bound redox reporter, methylene blue, binds at or near the ligand binding site on the aptamer resulting in a folded conformation of the cocaine‐binding aptamer. Addition of ligand then competes with the redox reporter for binding, altering its electron transfer rate. While we show this for the cocaine‐binding aptamer, given the prevalence of methylene blue in E‐AB sensors, a similar competition‐based may occur in other systems.
Calculating porosity and permeability from synthetic micro‐CT scan images based on a hybrid artificial intelligence
Authors: Mohyeddini Amir , Rasaei Mohammad Reza 
Published: March 2023

AbstractNowadays, lattice Boltzmann is one of the standard and exact methods of simulation in micro‐CT images of rock. However, it has a high weakness in run time. Therefore, the effort in this article is to reach a comprehensive substitute method for permeability calculation with less run time than the lattice Boltzmann method. The other purposes are the automation of processing operations, preparation of images, and in the end, the calculation of porosity. The best way to achieve these outcomes is to use hybrid artificial intelligence. In this research work, comprehensive model architecture has been used to design a hybrid artificial intelligence to be able to calculate permeability and porosity in complex images. A thousand images were randomly generated with high complexity, which makes the model comprehensive and extensible, and image processing was applied. After that, the lattice Boltzmann method as the direct simulation was selected. Finally, the convolutional neural network and multilayer perceptron based on a new and comprehensive model were evaluated for the first time; the mean squared error resulting from the evaluation of training data is 0.01, and the test data is 0.03. Expert systems have been used as a subset of artificial intelligence for automated image processing and porosity calculation. In this way, problems related to the direct implementation of classical algorithms for image processing, models, and patterns related to machine learning and needing an expert were solved to an acceptable extent, and an error of less than 5% was achieved.