bluuebunny/crossref_metadata_2025_split huggingface dataset:
Serum homocysteine level is an independent risk factor for 1-year stroke recurrence in patients with acute ischemic stroke and H-type hypertension: results from the Xi'an stroke registry study of China
Authors: Zhang Dandan , Liu Zhongzhong , Guo Weiyan , Lu Qingli , Zhang Huan , Lei Zhen , Liu Pei , Huang Congli , Wang Jing , Chang Qiaoqiao , Lin Xuemei , Wang Fang , Wu Songdi 
Published: April 2023

BackgroundH-type hypertension has a high prevalence in China. However, the association of serum homocysteine levels with 1-year stroke recurrence in patients with acute ischemic stroke (AIS) and H-type hypertension has not been studied.MethodsA prospective cohort study of patients with AIS admitted to hospitals between January and December 2015 in Xi'an, China, was conducted. Serum homocysteine levels, demographic data, and other relevant information were collected from all patients upon admission. Stroke recurrences were routinely tracked at 1, 3, 6, and 12 months after discharge. The blood homocysteine level was studied as a continuous variable and tertiles (T1–T3). A multivariable Cox proportional hazard model and a two-piecewise linear regression model were utilized to evaluate the association and ascertain the threshold effect regarding the serum homocysteine level and 1-year stroke recurrence in patients with AIS and H-type hypertension.ResultsOverall, 951 patients with AIS and H-type hypertension were enrolled, of whom 61.1% were male. After adjusting for confounders, patients in T3 had a significantly increased risk of recurrent stroke within 1 year, compared with those in T1 as the reference (hazard ratio = 2.24, 95% confidence interval: 1.01–4.97, p = 0.047). Curve fitting showed that serum homocysteine levels were positively curvilinearly correlated with 1-year stroke recurrence. Threshold effect analysis showed that an optimal threshold of serum homocysteine level &lt;25 μmol/L was effective in reducing the risk of 1-year stroke recurrence in patients with AIS and H-type hypertension. Elevated homocysteine levels in patients with severe neurological deficits on admission significantly increased the risk of 1-year stroke recurrence (p for interaction = 0.041).ConclusionsIn patients with AIS and H-type hypertension, the serum homocysteine level was an independent risk factor for 1-year stroke recurrence. A serum homocysteine level of ≥25 μmol/L significantly increased the risk of 1-year stroke recurrence. These findings can inform the creation of a more precise homocysteine reference range for the prevention and treatment of 1-year stroke recurrence in patients with AIS and H-type hypertension and provide a theoretical foundation for the individualized prevention and treatment of stroke recurrence.
Recovery sleep attenuates impairments in working memory following total sleep deprivation
Authors: Peng Ziyi , Hou Yanhong , Xu Lin , Wang Haiteng , Wu Shuqing , Song Tao , Shao Yongcong , Yang Yan 
Published: April 2023

IntroductionThe detrimental effects of sleep deprivation (SD) on cognitive function and quality of life are well known, and sleep disturbances are a major physical and mental health issue worldwide. Working memory plays an important role in many complex cognitive processes. Therefore, it is necessary to identify strategies that can effectively counteract the negative effects of SD on working memory.MethodsIn the present study, we utilized event-related potentials (ERPs) to investigate the restorative effects of 8 h of recovery sleep (RS) on working memory impairments induced by total sleep deprivation for 36 h. We analyzed ERP data from 42 healthy male participants who were randomly assigned to two groups. The nocturnal sleep (NS) group completed a 2-back working memory task before and after normal sleep for 8 h. The sleep deprivation (SD) group completed a 2-back working memory task before and after 36 h of total sleep deprivation (TSD) and after 8 h of RS. Electroencephalographic data were recorded during each task.ResultsThe N2 and P3 components—which are related to working memory—exhibited low-amplitude and slow-wave characteristics after 36 h of TSD. Additionally, we observed a significant decrease in N2 latency after 8 h of RS. RS also induced significant increases in the amplitude of the P3 component and in the behavioral indicators.DiscussionOverall, 8 h of RS attenuated the decrease in working memory performance caused by 36 h of TSD. However, the effects of RS appear to be limited.
Altered whole-brain gray matter volume in form-deprivation myopia rats based on voxel-based morphometry: A pilot study
Authors: Liu Jiayan , Lei Yahui , Diao Yuyao , Lu Yamei , Teng Xingbo , Chen Qingting , Liu Lian , Zhong Jingxiang 
Published: April 2023

BackgroundMyopia is one of the major public health problems worldwide. However, the exact pathogenesis of myopia remains unclear. This study proposes using voxel-based morphometry (VBM) to investigate potential morphological alterations in gray matter volume (GMV) in form-deprivation myopia (FDM) rats.MethodsA total of 14 rats with FDM (FDM group) and 15 normal controls (NC group) underwent high-resolution magnetic resonance imaging (MRI). Original T2 brain images were analyzed using VBM method to identify group differences in GMV. Following MRI examination, all rats were perfused with formalin, and immunohistochemical analysis of NeuN and c-fos levels was performed on the visual cortex.ResultsIn the FDM group, compared to the NC group, significantly decreased GMVs were found in the left primary visual cortex, left secondary visual cortex, right subiculum, right cornu ammonis, right entorhinal cortex and bilateral molecular layer of the cerebellum. Additionally, significantly increased GMVs were found in the right dentate gyrus, parasubiculum, and olfactory bulb.ConclusionsOur study revealed a positive correlation between mGMV and the expression of c-fos and NeuN in the visual cortex, suggesting a molecular relationship between cortical activity and macroscopic measurement of visual cortex structural plasticity. These findings may help elucidate the potential neural pathogenesis of FDM and its relationship to changes in specific brain regions.
STNet: A novel spiking neural network combining its own time signal with the spatial signal of an artificial neural network
Authors: Liu Fang , Tao Wentao , Yang Jie , Wu Wei , Wang Jian 
Published: April 2023

IntroductionThis article proposes a novel hybrid network that combines the temporal signal of a spiking neural network (SNN) with the spatial signal of an artificial neural network (ANN), namely the Spatio-Temporal Combined Network (STNet).MethodsInspired by the way the visual cortex in the human brain processes visual information, two versions of STNet are designed: a concatenated one (C-STNet) and a parallel one (P-STNet). In the C-STNet, the ANN, simulating the primary visual cortex, extracts the simple spatial information of objects first, and then the obtained spatial information is encoded as spiking time signals for transmission to the rear SNN which simulates the extrastriate visual cortex to process and classify the spikes. With the view that information from the primary visual cortex reaches the extrastriate visual cortex via ventral and dorsal streams, in P-STNet, the parallel combination of the ANN and the SNN is employed to extract the original spatio-temporal information from samples, and the extracted information is transferred to a posterior SNN for classification.ResultsThe experimental results of the two STNets obtained on six small and two large benchmark datasets were compared with eight commonly used approaches, demonstrating that the two STNets can achieve improved performance in terms of accuracy, generalization, stability, and convergence.DiscussionThese prove that the idea of combining ANN and SNN is feasible and can greatly improve the performance of SNN.
Prognostic value of neutrophil to lymphocyte ratio in gastric cancer patients receiving immune checkpoint inhibitors: a systematic review and meta-analysis
Authors: Zhang Siheng , Qiu Chao , Yu Hanzhi , Xu Yan , Xu Xiaoming 
Published: April 2023

BackgroundThe neutrophil to lymphocyte ratio (NLR) is a cost-effective and easily identifiable inflammatory biomarker that has been shown to be closely associated with tumor prognosis and predict survival in patients with multiple malignancies. However, the predictive value of NLR in patients with gastric cancer (GC) treated with immune checkpoint inhibitors (ICIs) has not been fully explored. Therefore, we conducted a meta-analysis to explore the potential of NLR as a predictor of survival in this population.MethodsWe systematically searched the PubMed, Cochrane Library, and EMBASE databases from inception to the present for observational researches on NLR and its relationship with progression or survival in GC patients receiving ICIs. To assess the prognostic significance of NLR on overall survival (OS) or progression-free survival (PFS), we used fixed or random-effect models to derive and combine hazard ratios (HRs) with 95% confidence intervals (CIs). We also examined the relationship between NLR and treatment efficacy by calculating relative risks (RRs) with 95% CIs for objective response rate (ORR) and disease control rate (DCR) in patients with GC receiving ICIs.ResultsNine studies of 806 patients were eligible. OS and PFS data were obtained from 9 and 5 studies, respectively. In nine studies, NLR was associated with poor survival, the pooled HR was 1.98 (95% CI 1.67- 2.35, p &lt; 0.001), indicating a significant association between high NLR and worse OS. We conducted subgroup analyses based on study characteristics to confirm the robustness of our findings. A relationship between NLR and PFS were reported in five studies with a HR of 1.49 (95% CI 0.99- 2.23, p = 0.056), which was not significantly associated. Pooling four studies that examined the correlation between NLR and ORR/DCR in GC patients, we observed a significant correlation between NLR and ORR (RR = 0.51, p = 0.003), but no significant correlation between NLR and DCR (RR = 0.48, p = 0.111).ConclusionIn summary, this meta-analysis indicates that increased NLR is significantly linked to worse OS in patients with GC receiving ICIs. In addition, lowering NLR can improve ORR. Thus, NLR can serve as a predictor for prognosis and treatment response in GC patients treated with ICIs. Nevertheless, further high-quality prospective studies are required to verify our findings in the future.
Echocardiographic and ultrasound evaluation of haemodynamic parameters in hypoxic neonates treated with hypothermia: Study protocol
Authors: Brunets Natalia , Brunets Veronika , Bokiniec Renata 
Published: April 2023

BackgroundEpisodes of ischaemia-hypoxia in the perinatal period as well as the changes in the redistribution of blood may lead to decreased perfusion and ischaemia of the cardiac muscle. Additionally, there is a negative impact from the reduced contractility of the cardiac muscle secondary to acidosis and hypoxia. Therapeutic hypothermia (TH) improves the late effects in moderate and severe cases of hypoxia-ischaemia encephalopathy (HIE). The direct impact of TH on the cardiovascular system includes moderate bradycardia, increased pulmonary vascular resistance (PVR), inferior filling of the left ventricle (LV) and LV stroke volume. The above-mentioned consequences of TH and episodes of HI in the perinatal period are therefore exacerbation of respiratory and circulatory failure. The impact of the warming phase on the cardiovascular system is not well researched and currently few data has been published on this topic. Physiologically, warming increases heart rate, improves cardiac output and increases systemic pressure. The effect of TH and the warming phase on the cardiovascular values has a decisive impact on the metabolism of drugs, including vasopressors/inotropics, which in turn affects the choice of medication and fluid therapy.MethodThe study is a multi-centre, prospective, case-control, observational study. The study will include 100 neonates (50 subjects and 50 controls). Echocardiography and cerebral and abdominal ultrasound will be performed in the first 1/2 days after birth as well as during warming i.e., on day 4/7 of life. In neonatal controls these examinations will be performed for indications other than hypothermia, most frequently because of poor adaptation.Ethics and disseminationThe Ethics Committee of the Medical University of Warsaw approved the study protocol prior to recruitment (KB 55/2021). Informed consent will be obtained from the carers of the neonates at the time of enrolment. Consent for participation in the study can be withdrawn at any time, without consequences and without obligation to justify the decision. All data will be stored in a secure, password-protected Excel file that is only accessible to researchers involved in the study. Findings will be published in a peer-reviewed journal and disseminated at relevant national and international conferences.Clinical Trial RegistrationNCT05574855.
Revealing the Mechanism of Huazhi Rougan Granule in the Treatment of Nonalcoholic Fatty Liver Through Intestinal Flora Based on 16S rRNA, Metagenomic Sequencing and Network Pharmacology
Authors: Liu Yingying , Tan Yingying , Huang Jiaqi , Wu Chao , Fan Xiaotian , Stalin Antony , Lu Shan , Wang Haojia , Zhang Jingyuan , Zhang Fanqin , Wu Zhishan , Li Bing , Huang Zhihong , Chen Meilin , Cheng Guoliang , Mou Yanfang , Wu Jiarui 
Published: April 2023

Background: The incidence of Nonalcoholic Fatty Liver (NAFL) is increasing year by year, growing evidence suggests that the intestinal flora plays a causative role in NAFL. Huazhi Rougan Granule (HRG) is commonly used in the clinical treatment of NAFL. It is reported that it can reduce lipids and protect the liver, but no research has confirmed whether the drug’s effect is related to the intestinal flora. Therefore, we investigated whether the effect of HRG is related to the regulation of intestinal flora to further explore the mechanism of HRG in the treatment of NAFL through intestinal flora.Methods: In this study, C57BL/6J mice were fed a high-fat diet for 10 weeks, and the high-fat diet plus HRG or polyene phosphatidylcholine capsules were each administered by gavage for 5 weeks. High-throughput sequencing, network pharmacology, and molecular docking were used to explore the mechanism of HRG in the treatment of NAFL through intestinal flora.Results: HRG treatment can reduce body weight gain, lipid accumulation in liver and lipogenesis and reduce serum biochemical indexes in high-fat-fed mice. Analysis of intestinal flora showed that HRG changed the composition of intestinal flora, which was characterized by a decrease in the Firmicutes/Bacteroidetes ratio. Moreover, the species distribution was significantly correlated with AKP, HDL-C, and TG. Metagenetic analysis showed that HRG altered the functional composition and functional diversity of microorganisms, which was mainly characterized by an increase in the abundance of metabolic pathways. The network pharmacology results show that the mechanism of HRG in the treatment of NAFL through intestinal flora is mainly reflected in the biological process of gene function and related to infectious diseases, immune systems, and signal transduction pathways, such as cytokine-cytokine receptor interaction, Chagas disease, IL-17 signaling pathway and other signaling pathways.Conclusion: These results strongly suggest that HRG may alleviate NAFL by preventing IFD.
A molecular network-based pharmacological study on the protective effect of Panax notoginseng rhizomes against renal ischemia–reperfusion injury
Authors: Li Dan-Dan , Li Na , Cai Chui , Wei Chun-Mian , Liu Guang-Hua , Wang Ting-Hua , Xu Fu-Rong 
Published: April 2023

Objective: We aimed to explore the protective effect of Panax notoginseng rhizomes (PNR) on renal ischemia and reperfusion injury (RIRI) and the underlying molecular network mechanism based on network pharmacology and combined systemic experimental validation.Methods: A bilateral RIRI model was established, and Cr, SCr, and BUN levels were detected. Then, the PNR was pretreated 1 week before the RIRI model was prepared. To determine the effects of the PNR in RIRI, histopathological damage and the effect of PNRs to the kidney was assessed, using TTC, HE, and TUNEL staining. Furthermore, the underlying network pharmacology mechanism was detected by screening drug–disease intersection targets from PPI protein interactions and GO and KEGG analysis, and the hub genes were screened for molecular docking based on the Degree value. Finally, the expression of hub genes in kidney tissues was verified by qPCR, and the protein expression of related genes was further detected by Western blot (WB).Results: PNR pretreatment could effectively increase Cr level, decrease SCr and BUN levels, reduce renal infarct areas and renal tubular cell injury areas, and inhibit renal cell apoptosis. By using network pharmacology combined with bioinformatics, we screened co-targets both Panax notoginseng (Sanchi) and RIRI, acquired ten hub genes, and successfully performed molecular docking. Of these, pretreatment with the PNR reduced the mRNA levels of IL6 and MMP9 at postoperative day 1 and TP53 at postoperative day 7, and the protein expression of MMP9 at postoperative day 1 in IRI rats. These results showed that the PNR could decrease kidney pathological injury in IRI rats and inhibit apoptotic reaction and cell inflammation so as to improve renal injury effectively, and the core network mechanism is involved in the inhibition of MMP9, TP53, and IL-6.Conclusion: The PNR has a marked protective effect for RIRI, and the underlying mechanism is involved in inhibiting the expression of MMP9, TP53, and IL-6. This striking discovery not only provides fruitful evidence for the protective effect of the PNR in RIRI rats but also provides a novel mechanic explanation.
Multi-criteria assessment to screen climate smart rice establishment techniques in coastal rice production system of India
Authors: Mohapatra Kiran Kumar , Nayak A. K. , Patra R. K. , Tripathi Rahul , Swain Chinmaya Kumar , Moharana K. C. , Kumar Anjani , Shahid Mohammad , Mohanty Sangita , Garnaik Saheed , Nayak Hari Sankar , Mohapatra Simran , Nagothu Udaya Sekhar , Tesfai M. 
Published: April 2023

IntroductionConventional rice production techniques are less economical and more vulnerable to sustainable utilization of farm resources as well as significantly contributed GHGs to atmosphere.MethodsIn order to assess the best rice production system for coastal areas, six rice production techniques were evaluated, including SRI-AWD (system of rice intensification with alternate wetting and drying (AWD)), DSR-CF (direct seeded rice with continuous flooding (CF)), DSR-AWD (direct seeded rice with AWD), TPR-CF (transplanted rice with CF), TPR-AWD (transplanted rice with AWD), and FPR-CF (farmer practice with CF). The performance of these technologies was assessed using indicators such as rice productivity, energy balance, GWP (global warming potential), soil health indicators, and profitability. Finally, using these indicators, a climate smartness index (CSI) was calculated.Results and discussionRice grown with SRI-AWD method had 54.8 % higher CSI over FPR-CF, and also give 24.5 to 28.3% higher CSI for DSR and TPR as well. There evaluations based on the climate smartness index can provide cleaner and more sustainable rice production and can be used as guiding principle for policy makers.
Using process features to investigate scientific problem-solving in large-scale assessments
Authors: Gong Tao , Shuai Lan , Jiang Yang , Arslan Burcu 
Published: April 2023

IntroductionThis study investigates the process data from scientific inquiry tasks of fair tests [requiring test-takers to manipulate a target variable while keeping other(s) constant] and exhaustive tests (requiring test-takers to construct all combinations of given variables) in the National Assessment of Educational Progress program.MethodsWe identify significant associations between item scores and temporal features of preparation time, execution time, and mean execution time.ResultsReflecting, respectively, durations of action planning and execution, and execution efficiency, these process features quantitatively differentiate the high- and low-performing students: in the fair tests, high-performing students tended to exhibit shorter execution time than low-performing ones, but in the exhaustive tests, they showed longer execution time; and in both types of tests, high-performing students had shorter mean execution time than low-performing ones.DiscussionThis study enriches process features reflecting scientific problem-solving process and competence and sheds important light on how to improve performance in large-scale, online delivered scientific inquiry tasks.
The role of booster vaccination in decreasing COVID-19 age-adjusted case fatality rate: Evidence from 32 countries
Authors: Zhou Cui , Wheelock Åsa M. , Zhang Chutian , Ma Jian , Dong Kaixing , Pan Jingxiang , Li Zhichao , Liang Wannian , Gao Jing , Xu Lei 
Published: April 2023

BackgroundThe global COVID-19 pandemic is still ongoing, and cross-country and cross-period variation in COVID-19 age-adjusted case fatality rates (CFRs) has not been clarified. Here, we aimed to identify the country-specific effects of booster vaccination and other features that may affect heterogeneity in age-adjusted CFRs with a worldwide scope, and to predict the benefit of increasing booster vaccination rate on future CFR.MethodCross-temporal and cross-country variations in CFR were identified in 32 countries using the latest available database, with multi-feature (vaccination coverage, demographic characteristics, disease burden, behavioral risks, environmental risks, health services and trust) using Extreme Gradient Boosting (XGBoost) algorithm and SHapley Additive exPlanations (SHAP). After that, country-specific risk features that affect age-adjusted CFRs were identified. The benefit of booster on age-adjusted CFR was simulated by increasing booster vaccination by 1–30% in each country.ResultsOverall COVID-19 age-adjusted CFRs across 32 countries ranged from 110 deaths per 100,000 cases to 5,112 deaths per 100,000 cases from February 4, 2020 to Jan 31, 2022, which were divided into countries with age-adjusted CFRs higher than the crude CFRs and countries with age-adjusted CFRs lower than the crude CFRs (n = 9 and n = 23) when compared with the crude CFR. The effect of booster vaccination on age-adjusted CFRs becomes more important from Alpha to Omicron period (importance scores: 0.03–0.23). The Omicron period model showed that the key risk factors for countries with higher age-adjusted CFR than crude CFR are low GDP per capita and low booster vaccination rates, while the key risk factors for countries with higher age-adjusted CFR than crude CFR were high dietary risks and low physical activity. Increasing booster vaccination rates by 7% would reduce CFRs in all countries with age-adjusted CFRs higher than the crude CFRs.ConclusionBooster vaccination still plays an important role in reducing age-adjusted CFRs, while there are multidimensional concurrent risk factors and precise joint intervention strategies and preparations based on country-specific risks are also essential.