bluuebunny/crossref_metadata_2025_split huggingface dataset:
The Application of Nucleic Acid Nanomaterials in the Treatment of Mitochondrial
Dysfunction
Authors: Yao Lan , Hai Qing , Zhang Tao 
Published: June 2023

Abstract:
Mitochondrial dysfunction is considered highly related to the development and progression of diseases,
including cancer, metabolism disturbance, and neurodegeneration. Traditional pharmacological approach for mitochondrial
dysfunction treatment has off-target and dose-dependent side effects, which leads to the emergence of
mitochondrial gene therapy by regulating coding or noncoding genes by using nucleic acid sequences such as oligonucleotides,
peptide nucleic acids, rRNA, siRNA, etc. To avoid size heterogeneity and potential cytotoxicity of the
traditional delivery vehicle like liposome, framework nucleic acids have shown promising potentials. First, special
spatial structure like tetrahedron allows entry into cells without transfection reagents. Second, the nature of nucleic
acid provides the editability of framework structure, more sites and methods for drug loading and targeted sequences
linking, providing efficient transportation and accurate targeting to mitochondria. Third, controllable size leads a
possibility to go through biological barrier such as the blood-brain barrier, reaching the central nervous system to
reverse mitochondria-related neurodegeneration. In addition, it's biocompatibility and physiological environmental
stability open up the possibility of in vivo treatments for mitochondrial dysfunction. Furthermore, we discuss the
challenges and opportunities of framework nucleic acids-based delivery systems in mitochondrial dysfunction.
Antioxidative and Anti-photooxidative Potential of Interruptins from the
Edible Fern Cyclosorus terminans in Human Skin Cells
Authors: Chaiwong Suriya , Sretrirutchai Somporn , Sung Jong-Hyuk , Kaewsuwan Sireewan 
Published: June 2023

Background:
Human skin is exposed daily to oxidative stress factors such as UV light,
chemical pollutants, and invading organisms. Reactive oxygen species (ROS) are intermediate
molecules that cause cellular oxidative stress. In order to survive in an oxygen-rich environment,
all aerobic organisms, including mammals, have evolved enzymatic and non-enzymatic defence
systems. The interruptins from an edible fern Cyclosorus terminans possess antioxidative properties
and can scavenge intracellular ROS in adipose-derived stem cells.

Objective:
This study aimed to evaluate the antioxidative efficacy of interruptins A, B, and C in
cultured human dermal fibroblasts (HDFs) and epidermal keratinocytes (HEKs). Moreover, the
anti-photooxidative activity of interruptins in ultraviolet (UV)-exposed skin cells was investigated.

Methods:
The intracellular ROS scavenging capacity of interruptins in skin cells was measured by
flow cytometry. Their induction effects on gene expression of the endogenous antioxidant enzymes
was monitored using real-time polymerase chain reaction.

Results:
Interruptins A and B, but not interruptin C, were highly effective in ROS scavenging,
particularly in HDFs. Interruptins A and B upregulated gene expression of superoxide dismutase
(SOD)1, SOD2, catalase (CAT), and glutathione peroxidase (GPx) in HEKs, but they only induced
SOD1, SOD2, and GPx gene expression in HDFs. Additionally, interruptins A and B efficiently
suppressed UVA- and UVB-induced ROS generation in both HEKs and HDFs.

Conclusion:
The results suggest that these naturally occurring interruptins A and B are potent
natural antioxidants and therefore may have the potential in the future of inclusion in antiaging
cosmeceutical products.
Low-frequency Electrical Stimulation of the Hippocampus Plays a Role in
the Treatment of Pharmacoresistant Epilepsy by Blocking the PKA-CREB
Pathway
Authors: Huang Yuanxin , Shi Dai , Wu Guofeng , Wang Likun , Ren Siying 
Published: June 2023

Objective:
The objective of this study is to study the mechanism of Low frequency electrical
stimulation (LFS) in the treatment of drug-resistant epilepsy by regulating the protein kinase
A (PKA)-cAMP response element-binding protein (CREB) signaling pathway upstream of gamma
aminobutyric acid A (GABAA) receptor.

Methods:
Primary hippocampal neurons were extracted and cultured from fetal rat brains and
randomly divided into the normal control group, PKA-CREB agonist group, and PKA-CREB
inhibitor group. Drug-resistant epileptic rats were established and randomly divided into the
pharmacoresistant group, LFS group, PKA-CREB agonist combined with hippocampal LFS group,
and PKA-CREB inhibitor combined with hippocampal LFS group. The normal rats were in
the normal control group and drug-sensitive rats were in the pharmacosensitive group. The seizure
frequency of epileptic rats was determined using video surveillance. The expression of
PKA, CREB, p-CREB, and GABAA receptor subunits α1 and β2 of each group were detected
using reverse transcription quantitative polymerase chain reaction (RT-qPCR) and western blotting
assays.

Results:
The in vitro expression levels of PKA, CREB, and p-CREB in the agonist group were
significantly higher than those in the normal control group (NRC group), while the expression levels
of GABAA receptor subunits α1 and β2 were significantly lower than those in the NRC group.
The expression levels of PKA, CREB, and p-CREB in the inhibitor group were significantly lower,
while the expression levels of GABAA receptor subunits α1 and β2 were significantly higher than
those in the NRC group. The in vivo seizure frequency was significantly lower in the LFS group
than in the pharmacoresistant group (PRE group). Compared to the LFS group, the seizure frequency
and the expression levels of PKA, CREB, and p-CREB in the rat hippocampus were significantly
higher, and the expression levels of GABAA receptor subunits α1 and β2 were significantly
lower in the agonist group. The results in the inhibitor group were exactly the opposite of those in
the agonist group.

Conclusion:
The PKA-CREB signaling pathway is involved in the regulation of GABAA receptor
subunits α1 and β2. In addition, LFS plays an important role in increasing GABAA receptor expression
by regulating the PKA-CREB signaling pathway.
Early Blockage of Mycobacterium Tuberculosis Cell-wall Synthesis via
EchA\6 Inhibition to Overcome Resistance Strain: Insights from Umbrella
Sampling Simulations
Authors: Askandar Rafee Habib , Sharifi Farhad , Shayan Sepideh , Mohammadi Helya , Rahimi Arian , Ardalan Noeman , Mohammed Heshw Farhad 
Published: June 2023

Background:
Tuberculosis (TB) has long been the major infectious cause of mortality, ranking higher than HIV/AIDS as the most common cause of death from a single infectious agent worldwide. The EchA6 target of mycobacteria plays a vital role in synthesizing an important component of the mycobacterial outer membrane. The failure of TB treatment has prompted the investigation of novel anti-tubercular drugs.

Objective:
This study was aimed at blockage of Mycobacterium tuberculosis cell-wall synthesis via EchA6 inhibition to overcome resistance strain.

Methods:
Over 3,000,000 compounds and GSK951A (positive control) were investigated as the
inhibitors in this study. The GROMACS molecular dynamic package was used to analyze the protein-
inhibitor complex's conformational changes under constant temperature and pressure. Also,
umbrella sampling (US) was used for free binding energy (ΔG) calculation.

Results:
Four compounds were chosen for the docking investigation. According to the MD analysis, the studied inhibitors demonstrated good stability and flexibility. According to ∆G obtained from US, the ∆G of GSK951A, ZINC11815220, ZINC67770050, ZINC55048326, and ZINC89700914 were -6.14 kcal mol-1, -5.25 kcal mol-1, -10.19 kcal mol-1, -8.55 kcal mol-1, and -8.37 kcal mol-1, respectively.

Conclusion:
In conclusion, ZINC67770050 is recommended for further study in the laboratory. This investigation is an important starting point for discovering anti-tubercular drugs using EchA6 inhibition.
The Analgesic Effect of Ginger on Postoperative Pain: A Systematic
Review of Clinical Trials
Authors: Farsani Hamidreza Azizi , Heidari-Soureshjani Saeid , Sherwin Catherine MT , Tafrishinejad Arash , Azadegan-Dehkordi Zahra 
Published: June 2023

Introduction and Aim:
Pain is a common problem that can negatively affect patients' daily life
and impair the quality of life of patients. This systematic review evaluates ginger's analgesic effects and
underlying mechanisms in postoperative pain.

Methods:
An extensive search was undertaken in various databases, including Cochrane Library, Pub-
Med, Embase, Web of Science, and Scopus. After considering the study's inclusion and exclusion criteria,
12 records were retrieved. The raw data were extracted and entered into an Excel form, and the study
outcomes were analyzed. A PRISMA 2020 flow diagram illustrates the direct search approach used for
this systematic review.

Results:
The reviewed studies mainly examined ginger's analgesic effects and other chemical analgesics,
such as ibuprofen. Ginger and its bioactive compounds, such as gingerols and shogaols, can reduce postoperative
pain by relieving nociceptive, mechanical, and neuropathic pain inflammatory pain by activating
the various descendent inhibitory pathways of pain. Ginger induces its postoperative analgesic effects by
involving and changing thinly myelinated A-delta, unmyelinated C-fibers, and myelinated A-beta-fibers,
Transient receptor potential vanilloid 1 (TRPV1), and inhibiting inflammatory process and oxidant activities.

Conclusion:
Ginger is emerging as promising analgesic effects through various nociceptive pathways on
postoperative pain in patients. Additional rigorous clinical trials are warranted to investigate these results
further.
Esophageal Foreign Body Removal by Bile Duct Stone Removal Baskets
(Litho crush): Think out of the Box; A Case Report
Authors: Jat Shankar Lal , Mathur Amit 
Published: June 2023

Abstract:
A foreign body or food bolus impaction in the gastrointestinal tract is a common occurrence in emergency endoscopy. In 80-90% of patients, the foreign body will pass spontaneously through the gastrointestinal tract without complications, and in 10-20% of the patients, an endoscopic or surgical intervention is deemed necessary. We report an unusual case of a foreign body, a large mango seed, that was stuck at the level of the mid-esophagus and was refractory to removal by standard endoscopic techniques.
Survival and Trends in Annualized Hazard Function by Age at Diagnosis Among Chinese Breast Cancer Patients Aged ≤40 Years: Case Analysis Study
Authors: Wang Jun , Luo Ting , Xiang Zhong-zheng , He Ming-min , Zeng Yuan-yuan , Yang Tian , Wei Xiao-yuan , Yu Siting , Dai Ze-lei , Xu Ning-yue , Tan Chen-feng , Liu Lei 
Published: June 2023

Background
Young breast cancer patients are more likely to develop aggressive tumor characteristics and a worse prognosis than older women, and different races and ethnicities have distinct epidemiologies and prognoses. However, few studies have evaluated the clinical biological features and relapse patterns in different age strata of young women in Asia.

Objective
We aimed to explore survival differences and the hazard function in young Chinese patients with breast cancer (BC) by age.

Methods
The patients were enrolled from West China Hospital, Sichuan University. The chi-squared test, a Kaplan-Meier analysis, a log-rank test, a Cox multivariate hazards regression model, and a hazard function were applied for data analysis. Locoregional recurrence–free survival (LRFS), distant metastasis–free survival (DMFS), breast cancer–specific survival (BCSS), and overall survival (OS) were defined as end points.

Results
We included 1928 young BC patients diagnosed between 2008 and 2019. Patients aged 18 to 25, 26 to 30, 31 to 35, and 36 to 40 years accounted for 2.7% (n=53), 11.8% (n=228), 27.7% (n=535), and 57.7% (n=1112) of the patients, respectively. The diagnosis of young BC significantly increased from 2008 to 2019. Five-year LRFS, DMFS, BCSS, and OS for the entire population were 98.3%, 93.4%, 94.4%, and 94%, respectively. Patients aged 18 to 25 years had significantly poorer 5-year LRFS (P<.001), 5-year DMFS (P<.001), 5-year BCSS (P=.04), and 5-year OS (P=.04) than those aged 31 to 35, 26 to 30, and 36 to 40 years. The hazard curves for recurrence and metastasis for the whole cohort continuously increased over the years, while the BC mortality risk peaked at 2 to 3 years and then slowly decreased. When stratified by age, the annualized hazard function for recurrence, metastasis, and BC mortality in different age strata showed significantly different trends, especially for BC mortality.

Conclusions
The annual diagnosis of young BC seemed to increase in Chinese patients, and the distinct age strata of young BC patients did not differ in survival outcome or failure pattern. Our results might provide strategies for personalized management of young BC.
Implementasi Kontrol PID pada Kopel Motor DC dengan Menggunakan Filter Kalman
Authors: Nurrahma Salima , Berbudi Tegar Arif , Firdaus Muhammad Rakha , Izzaulhaq Galang , Hudati Imroatul 
Published: June 2023

This paper describes a DC motor clutch system that uses control methods PID and uses a Kalman filter. The methods used aim to obtain stable results from the DC motor coupling system used. To get the appropriate results, several stages were carried out such as hardware design where this process was carried out by pairing all the components used including the power supply, DC motor driver, Arduino Uno, potentiometer and the two coupled DC motors. This system uses an input voltage of 12 V and will then be processed by the motor driver to drive the DC motor. Based on the results that have been obtained and the system response after being given control. It can be observed that when the system uses the Kalman filter and uses the PI control the system response results have a small steady state error.
The Role of Career Women in Creating a Sakinah Family: From Mubadalah (Mutuality) Perspective
Authors: Nastangin Nastangin , Huda Muhammad Chairul 
Published: June 2023

Abstract: The Role of Career Women In Creating A Sakīnah Family: From Mubādalah (Mutuality) Perspective. Career women have dual roles, first as a wife and second as a worker. This dual role, either directly or indirectly, will affect their ability to manage domestic life. This study tries to examine this phenomenon through the perspective of mubādalah (mutuality) from Faqihuddin Abdul Kodir. The aim is to find out the success/failure of how career women reconcile their roles as housewives as well as career women. This research is qualitative by using a socio-legal approach. Data were collected using in-depth interview techniques conducted with several female lecturers at IAIN Salatiga. This study finds the fact that the perspective of Mubādalah (Mutuality) theory, when drawn into the realm of family life of female lecturers at IAIN Salatiga, is in line because Mubādalah theory requires a balance of relations between husband and wife and the elimination of gender bias to create a harmonious and prosperous family life.
An Appraisal of The Legality of Translating Jumu’ah Khutbah in Nigeria
Authors: Mustapha Abdur-Razzaq Solagberu Balogun Mustapha Balogun 
Published: June 2023

Khutbah is one the stipulated condition that distinguish Jumucah prayer from other obligatory five ritual daily prayers. By the time Islam spread to what is now known as Nigeria, the Khutbah used to be rendered in Arabic without any attempt to translate it to any local language. However, with passage of time the population of Muslims continues to increase. As a result of that, there are agitations and counter-agitation on the need to either translate the khutbah into a local languages or to render it in a vernacular. The issue of legality comes in as a means of justifying either of the duo agitations. The objective of this research paper is to examine the legality or otherwise of translating the khutbah especially in a country like Nigeria where a section of the Muslims who observe their Jumu’ah prayer could not comprehend the language in which the khutbah is being delivered. The method adopted in the research is based on both participatory and non-participatory observations. The end-result of the research reveals that the khutbah can be legally translated considering a large population of the worshippers who could not comprehend the language of communication i.e. Arabic. By extention, the khutbah of religious festivals such as cIdul-Fitr and cIdul-Adha be translated too. Similarly ceremonial events where Khutbah is rendered in Arabic should also be translated. All these will afford the larger number of the congregation not only to be exposed to the stand and teachings of Islam on current issues but will also enable them to appreciate the teachings of Islam.
Penerapan Model Diskursus Multy Representasi : Dampaknya terhadap Kemampuan Pemahaman Konsep Matematis dan Self Eficacy
Authors: Amani Fauziyah , Pratiwi Dona Dinda , Anggoro Bambang Sri 
Published: June 2023

Abstract:The Multy Representation Discourse is a learning model that emphasizes students learning in groups and the use of representation. This study aims to determine the influence of the DMR model on the ability to understand mathematical concepts and the self-efficacy of students. The research design uses Quasy Experimental Design with a posttest-only control design. The population was all class VIII students at one of the MTs in Krui. The sample number of 50 students consisted of 25 in the experimental class and 25 in the control class taken by the cluster random sampling technique. Hypothesis testing using Multivariate Analysis of Variance (Manova). The results showed that students who learned with the DMR learning model had better concept understanding and self-efficacy skills than students who learned using the direct learning model.Abstrak:Diskursus Multy Representasi (DMR) merupakan model pembelajaran yang menekankan peserta didik untuk belajar berkelompok dan pemanfaatan representasi. Penelitian bertujuan mengetahui pengaruh model DMR terhadap kemampuan pemahaman konsep matematis dan self efficacy peserta didik. Rancangan penelitian menggunakan Quasy Experimental Design dengan posttest-only control design. Populasi penelitian ini adalah seluruh peserta didik kelas VIII pada salah satu MTs di Krui. Jumlah sampel 50 peserta didik terdiri dari 25 di kelas eksperimen dan 25 di kelas kontrol yang diambil dengan teknik cluster random sampling. Pengujian hipotesis menggunakan Multivariate Analysis of Variance (Manova). Hasil penelitian menunjukkan Peserta didik yang belajar dengan model pembelajaran DMR memiliki kemampuan pemahaman konsep dan self efficacy yang lebih baik dibandingkan peserta didik yang belajar menggunakan model pembelajaran langsung.
Investigasi Desain Pengajaran untuk Penalaran Matematis
Authors: Fatmiyati Novita , Prasetyo Freddy , Ambarwati Dela , Dasari Dadan 
Published: June 2023

Abstract:Learning mathematics requires students to have various abilities and skills, including mathematical reasoning ability. This study aims to identify any matters related to teaching design in mathematics education toward students' mathematical reasoning abilities. The method used was Systematic Literature Review (SLR) and the collection of literature used as research subjects were carried out by searching the Scopus database and using inclusion criteria in the form of (1) written in English, (2) published in the last ten years (2013-2022), and (3) indexed by Scopus. There are 24 articles identified that meet the inclusion criteria and were analyzed based on teaching design categorization by van der Akker that has been modified by Lithner. The results found that there are learning designs that affect various mathematical reasoning abilities, namely the Zone of Proximal Development (ZPD), Realistic Mathematics Education (RME), and Theory of Didactical Situation (TDS), where various claims in the form of interventions such as learning models, learning instructions and teaching media or materials, which have been seen to effectively improve students' mathematical reasoning abilities. Abstrak:Pembelajaran matematika menuntut kemampuan dan keterampilan siswa, salah satunya kemampuan penalaran matematika. Penelitian ini bertujuan mengindentifikasi desain pembelajaran matematika terkait kemampuan penalaran matematis siswa. Metode yang digunakan yaitu Systematic Literature Review (SLR) dengan mengidentifikasi database Scopusdengan kriteria inklusi berupa (1) ditulis dalam Bahasa Inggris, (2) diterbitkan selama sepuluh tahun terakhir (2013-2022), dan (3) terindeks Scopus. Terdapat 24 artikel yang teridentifikasi memenuhi kriteria tersebut yang selanjutnya dianalisis berdasarkan kategorisasi desain pengajaran menurut van der Akker yang dimodifikasi oleh Lithner. Hasil penelitian ini menemukan terdapat desain pengajaran yang mempengaruhi berbagai kemampuan penalaran matematis, yaitu Zone of Proximal Development (ZPD), Realistic Mathematics Education (RME), dan Theory of Didactical Situation (TDS), dengan berbagai klaim diantaranya intervensi model pembelajaran, instruksi pembelajaran, dan media atau bahan ajar, yang secara efektif meningkatkan kemampuan penalaran matematika siswa.
Computed tomographic laryngotracheal dimensions in adult domestic rabbits (Oryctolagus cuniculus) are positively associated with body weight and the laryngotracheal lumen is narrowest at the level of the thyroid cartilage
Authors: Arendt Tessa B. , Loeber Samantha J. , Schroeder Carrie A. , Lasarev Michael R. , Ferreira Tatiana H. 
Published: June 2023

Abstract

OBJECTIVE
This retrospective study aimed to measure rabbit laryngotracheal dimensions at different locations on computed tomography (CT), assess the relationship of these measurements with rabbit body weight, determine the most common narrowest measurement and assess its relationship with endotracheal tube (ETT) size and body weight.

ANIMALS
66 adult domestic rabbits (Oryctolagus cuniculus) of different breeds and body weights.

PROCEDURES
CT laryngotracheal luminal height, width, and cross-sectional area measurements were made at the rostral thyroid cartilage at the level of the arytenoids, caudal thyroid cartilage/rostral cricoid cartilage, caudal cricoid cartilage/cranial trachea, and trachea at the level of the fifth cervical vertebra.

RESULTS
The data for every measurement of luminal airway dimensions revealed robust positive associations with body weight (P < .001). The narrowest laryngotracheal measurement was the width at the level of the caudal thyroid cartilage/rostral cricoid cartilage, and the smallest cross-sectional area was at the rostral thyroid cartilage at the level of the arytenoids. There was a strong association between body weight and the likelihood of appropriate ETT fit. To have at least an 80% chance of appropriate ETT fit with a 2.0, 2.5, and 3.0 mm ETT, the rabbits’ weight predicted by the model (lower 95% confidence limit) were at least 2.99 (2.72) kg, 5.24 (4.65) kg, and 5.80 (5.21) kg, respectively.

CLINICAL RELEVANCE
The laryngotracheal lumen was narrowest at the level of the caudal thyroid cartilage in rabbits, which indicates this location may be the limiting factor in determining ETT size in rabbits.