bluuebunny/crossref_metadata_2025_split huggingface dataset:
Using energy to go downhill – a genoprotective role for ATPase activity in DNA topoisomerase II
Authors: Bandak Afif F. , Blower Tim R. , Nitiss Karin C. , Shah Viraj , Nitiss John L. , Berger James M. 
Published: June 2023

SummaryType II topoisomerases effect topological changes in DNA by cutting a single duplex, passing a second duplex through the break, and resealing the broken strand in an ATP-coupled reaction. Curiously, most type II topoisomerases (topos II, IV, and VI) catalyze DNA transformations that are energetically favorable, such as the removal of superhelical strain; why ATP is required for such reactions is unknown. Here, using human topoisomerase II β (hTOP2β) as a model, we show that the ATPase domains of the enzyme are not required for DNA strand passage, but that their loss leads to increased DNA nicking and double strand break formation by the enzyme. The unstructured C-terminal domains (CTDs) of hTOP2β strongly potentiate strand passage activity in the absence of the ATPase regions, as do cleavage-prone mutations that confer hypersensitivity to the chemotherapeutic agent etoposide. The presence of either the CTD or the mutations lead ATPase-less enzymes to promote even greater levels of DNA cleavaingevitro, as well asin vivo. By contrast, the aberrant cleavage phenotypes of these topo II variants is significantly repressed when the ATPase domains are restored. Our findings are consistent with the proposal that type II topoisomerases acquired an ATPase function to maintain high levels of catalytic activity while minimizing inappropriate DNA damage.
Hepatoblastoma exhibits a predominantly myeloid immune landscape and reveals opportunities for macrophage targeted immunotherapy
Authors: Krijgsman Daniëlle , Kraaier Lianne , Verdonschot Meggy , Schubert Stephanie , Leusen Jeanette , Duiker Evelien , de Kleine Ruben , de Meijer Vincent , de Krijger Ronald , Zsiros József , Peng Weng Chuan , Vercoulen Yvonne 
Published: June 2023

AbstractBackground & AimsHepatoblastoma (HB) is a rare form of pediatric liver cancer which is currently treated with chemotherapy and surgery. The side effects of chemotherapy pose a major problem in HB and underline the need for an alternative treatment option. We aimed to characterize the immune landscape of HB to improve our understanding of the immunologic contribution to this disease and explore immunotherapeutic options.MethodsAn imaging mass cytometry panel of 36 antibodies was used on tissue of treatment-naive HB (n=5), and chemotherapy-treated HB (n=3), with paired distal normal liver tissue. Immunofluorescence was used to stain HB and normal liver tissue for Kupffer cell marker MARCO. A public single-cell RNA-sequencing (scRNA-seq) dataset was analyzed consisting of 9 chemotherapy-treated HB and paired normal liver tissue.ResultsHB showed a heterogeneous immune landscape predominantly comprising macrophages and monocytes with high expression of immune checkpoints CD47, SIRPα, and VISTA, whereas T cells were limited. Chemotherapy increased influx of macrophages and CD8+T cells in HB. Transcriptome profiling demonstrated an early activated phenotype of CD8+T cells in chemotherapy-treated HB and absence of an exhaustion signature and immune checkpoint expression. Furthermore, tumor-associated macrophages had lowMARCOexpression, upregulated inflammatory markers and a high liver tissue residency score while expressing other Kupffer cell markers, such asCD5L, to a variable degree.ConclusionsThe absence of immune checkpoints and exhaustion markers in CD8+T cells prohibits T cell-targeting by immune checkpoint blockade in HB patients. Instead, HB tumors contain a large myeloid compartment which provide opportunities for macrophage targeting, thereby paving the way for the development of improved treatment strategies for HB patients.Graphical abstract
The Tubulin Nano-Code: a protofilament-specific pattern of tubulin post-translational modifications regulates ciliary beating mechanics
Authors: Viar G. Alvarez , Klena N. , Martino F. , Nievergelt A. , Pigino G. 
Published: June 2023

AbstractControl of ciliary beating is crucial for motility and signaling in eukaryotic cells and requires spatially restricted interactions between axonemal proteins and specific protofilaments within the ciliary microtubules. How these interactions are regulated remains poorly understood, but increasing evidence indicates that tubulin post-translational modifications (tPTMs) are required for proper ciliary motility. The Tubulin Code refers to the concept that tPTMs can modulate the function of individual microtubules in cells. Here we use a combination of immuno-cryo-electron tomography, expansion microscopy and mutant analysis to show that, in motile cilia, tubulin glycylation and polyglutamylation form mutually exclusive protofilament-specific nano-patterns at sub-microtubular scale. We show that these two nano-patterns are consistent with the distributions of axonemal dyneins and nexin-dynein regulatory complexes, respectively, and are required for their regulation during ciliary beating. Our discovery of a tubulin nano-code in cilia highlights the need of higher-resolution studies also in other cellular compartments to understand the molecular role of tPTMs.One-Sentence SummaryTubulin post-translational modifications form nanopatterns at sub-microtubular scale that enable individual protofilaments to perform specific functionsGraphical Abstract
Characterization and corrosion behavior of Ni-Cr coatings by using pulse current electrodeposition
Authors: Zhu Yesen , Gu Cheng-Qing , Wang Jinliang , Xi Xiaohui , Qin Zhenbo 
Published: June 2023

Purpose
The purpose of this paper is to study the effect of chromium content on the microstructure and corrosion resistance of Ni-Cr coating.

Design/methodology/approach
Ni-Cr coating was prepared by pulse current electrodeposition with trivalent chromium. On the basis of studying effect of electroplating parameters on composition and morphology, Ni-Cr alloy coatings with various chromium contents were obtained. The microstructure was characterized by scanning electron microscopy, X-ray diffractometer and transmission electron microscopy. Corrosion behavior was studied by potentiodynamic polarization and electrochemical impedance spectroscopy techniques.

Findings
Electrodeposited chromium was solidly dissolved in nickel and refined the grain of the coating. With the increase of Cr content, the corrosion resistance of Ni-Cr coating was enhanced, which is due to the formation of continuous nickel hydroxide and compact chromium oxide passive films.

Originality/value
Ni-Cr alloy coating without penetration crack was prepared in trivalent chromium electrolyte, and the mechanism of its excellent corrosion resistance was proposed.
Stereotypes, same-sex struggles, and sustainable shopping: intrasexual competition mediates sex differences in green consumption values
Authors: Otterbring Tobias 
Published: June 2023

PurposeThe present study aimed to investigate whether male consumers report weaker green consumption values than their female counterparts, and whether such a presumed sex difference, at least in part, can be explained by different levels of intrasexual competition. In other words, the study tested the notion that intrasexual competition acts as a psychological mechanism explaining why male (vs female) consumers are sometimes less prone to prefer and purchase sustainable goods, with their higher tendency to compete with same-sex rivals making them less likely to engage in green consumption.Design/methodology/approachThe study was based on a large cross-sectional survey, in which a final sample of 1,382 participants (823 female and 559 male) provided complete responses on well-validated scales measuring intrasexual competition and green consumption values. The large sample size implies that even small effect sizes could be detected with high statistical power. The data were analyzed using a series of Mann–Whitney U tests to compare the responses made by male and female participants. Subsequently, multiple linear regressions as well as regression-based mediation and moderation analyses were performed with control variables added to show robustness of the results, test the proposed chain of events, and demonstrate generalizability.FindingsMale (vs female) participants expressed significantly higher levels of intrasexual competition both generally and on the two subdimensions corresponding to superiority striving and inferiority irritation. Further, they were slightly less inclined to express green consumption values. Importantly, the sex difference in green consumption values was mediated by inferiority irritation as well as the entire intrasexual competition scale but not by superiority striving. Thus, men's inferiority irritation, in particular, and their more pronounced propensity to compete with same-sex rivals, in general, drove them away from green consumption, whereas women's weaker willingness to compete with same-sex rivals instead increased their inclination of “going green.”Originality/valueDrawing on findings from the domains of competitiveness and gender stereotypes, the current research demonstrates a novel mechanism through which green consumption responses can be understood. Specifically, this study provides empirical evidence for the mediating role of intrasexual competition, especially regarding the more negatively charged subdimension of inferiority irritation, in explaining why male and female consumers may differ in terms of their green consumption values. The present research also contributes to the literature by questioning the unidimensional structure of the intrasexual competition scale and showing that the negative (vs positive) subdimension of this scale is more influential in explaining sex-differentiated patterns in consumers' green consumption values, thereby supporting the notion that “bad is stronger than good.”
Design for adaptability and disassembly: guidelines for building deconstruction
Authors: Munaro Mayara Regina , Tavares Sergio Fernando 
Published: June 2023

Purpose
Design for adaptability and disassembly (DfAD) is an effective method to reduce construction and demolition waste generation, landfill loads and greenhouse gas generation; preserve natural resources; and increase environmental awareness in the construction industry. However, it is an underexplored strategy due to a lack of information about projects and a set of agreed guidelines to guide buildings deconstruction. This study aims to understand how DfAD can support the sector’s transition toward circularity.

Design/methodology/approach
Through an integrative literature review, this study analyzed the current publications and terminologies used, identified the main themes discussed and described the key criteria for integrating deconstruction in the building design stage.

Findings
The results showed that the term DfAD encompasses different ecodesign strategies and is concentrated in six major thematic categories (design and construction principles, tools for DfAD, components and connections for DfAD, barriers, drivers and guidelines for DfAD, existing building stock potential and selective deconstruction process). In total, 60 criteria were presented to guide the deconstruction of buildings, emphasizing standardization, modularization and prefabrication of materials and components as fundamental requirements.

Originality/value
The study highlighted the need to expand the knowledge and training of the design team, establish public policies and tax incentives and develop tools, methods, and circular indicators to enable the implementation of deconstruction strategies for buildings.
Minimal mass of prismatic tensegrity structures
Authors: Cao Ziying , Luo Ani , Feng Yaming , Liu Heping 
Published: June 2023

PurposeThis paper is contributed to find the minimal mass prismatic tensegrity structures.Design/methodology/approachIn the stable state of the structure with any given external force, the internal forces of the structure members are taken as the critical force to calculate the cross-sectional area, and the total mass of the structure can be obtained. Firstly, the mathematical model of prismatic tensegrity was built. Secondly, the stability of the tensegrity was analyzed based on the force equilibrium of one node, the force density relationship of elements was obtained. The deformation of p-bar tensegrity prism unit was studied with the same mass. The force of the structure under external force was analyzed.Findings(1) The length of bar and the structural radius are almost invariant, and the mechanical properties of 3-bar tensegrity prism is more outstanding; (2) theoretically, the mass of the structure is minimal while the projection of bar passes the center of the circle. Under the circumstances, the force of diagonal cable is 0 N, the vertical force component of bar cancels the axial external force.Originality/value(1) By analyzing the deformation of p-bar tensegrity prism with the same mass, the length of bar and the structural radius are proved be almost invariant and the mechanical properties of 3-bar tensegrity prism is more outstanding; (2) theoretically, the mass of the structure is minimal while the projection of bar passes the center of the circle. Under the circumstances, the force of diagonal cable is 0 N, the vertical force component of bar cancels the axial external force.
Digitalization of information provided by destination marketing organizations in developing regions: the case of Vietnamese Mekong Delta
Authors: Huynh Da Van , Stangl Brigitte , Thi Tran Dieu 
Published: June 2023

PurposeThis research aims to investigate how emerging destinations cope with digitalization of information, where they are in the process and how digitalization of information takes place in destination marketing organizations (DMOs). As a case for emerging destinations that must deal with the negative consequences of the digital divide, the Vietnamese Mekong Delta (VMD) will be examined. A new framework, solutions in general, and potential innovative approaches will be presented.Design/methodology/approachA mixed methods approach was used. Firstly, a content analysis comprising 68 criteria to examine 10 destination websites was conducted to evaluate the performance of provincial destination websites of VMD. Secondly, the authors interviewed five managers from VMD DMOs to reveal the strategy, status quo and their challenges with digitalization.FindingsSome digitalization is evident in VMD DMOs, with the digitization of tourist information provision developing from analog formats to digital modes. The content analysis of the websites shows that provincial destination websites of VMD perform well with regard to communication but need improvements for transaction, and especially relationship aspects. Emerging destinations like VMD DMOs are reaching the second or third level in the digitalization process. Yet they face challenges with human and financial resources.Practical implicationsThis research provides recommendations concerning destination website performance, the process of digitalization and how to promote digitalization and apply more digital instruments to move to the next stages of destination digitalization. Also, suggestions on how to overcome existing challenges/barriers in similar areas of the world are provided.Originality/valueA new, extended more granulated version of the digitalization framework by Karpova et al. (2019) has been developed. The new model acknowledges the continued importance of printed information, provides information about the sequence of steps how to implement website dimensions, and which instruments are realistic to implement in different levels of digitalization considering the challenges and barriers developing destinations face.
Bandgap mechanisms and wave characteristics analysis of a three-dimensional elastic metastructure
Authors: Li Yingli , Wu Chenwei , Peng Yong , Jiang Xudong 
Published: June 2023

PurposeIn order to investigate the vibration reduction properties of a three-dimensional elastic metastructure with spherical cavities at low frequencies.Design/methodology/approachThe bandgap characteristics of a three-dimensional elastic metastructure with spherical cavities are studied based on analytical and numerical approaches.FindingsThe results of both method revealed that the vibration of the vertexes masses is important for opening bandgaps. The fact that the big sphere cavity radius or short side length of the cube unit leads to a wider bandgap, is noteworthy.Originality/valueThis research provides theoretical guidance for realizing the vibration attenuation application of EMs in practical engineering.
Effect of laser scanning speed on microstructure and corrosive-wear performance of Ni-60%WC coating in Wusu mine water
Authors: Chuang He , Fan Wang , Zhicheng Liu , Dejun Kong 
Published: June 2023

Purpose
The purpose of this study is to investigate the effect of laser scanning speed (LSS) on the corrosive-tribological performance of Ni-60%WC coating in Wusu mine water, which was beneficial to improve the friction–wear performance of cylinder liner on water injection pump.

Design/methodology/approach
Ni-60%WC coatings were fabricated on 45 steel by laser cladding, and the microstructure and tribological performance was analyzed using a super depth of field microscope and ball-on-plate friction tester, and the wear mechanism was also discussed.

Findings
At room temperature (RT, 25 ± 2 °C), the average coefficients of friction of substrate and Ni-60%WC coatings fabricated at the LSS of 6, 10, 12 and 14 mm/s are 0.48 ± 0.08, 0.23 ± 0.01, 0.21 ± 0.05, 0.22 ± 0.02 and 0.25 ± 0.04, respectively, and the corresponding wear rates are 8.755 × 104, 4.525 × 103, 1.539 × 103, 1.957 × 103 and 2.743 × 103 µm3·s–1·N–1, respectively, showing that the coating fabricated at the LSS of 10 mm/s has best friction reduction and wear resistance. The wear mechanism of Ni-60%WC coating is abrasive wear, fatigue wear and oxidative wear, which is resulted from the WC particles with the high-hardness.

Originality/value
Ni-60%WC coatings were first applied for cylinder liner, and the effect of laser scanning speed on its tribological performance was investigated.
Find their country-of-origin Facebook and cross the threshold of overseas legitimacy: a multi-case study of Chinese enterprises
Authors: Zheng Supeng , Xu Yusen , Lin Haifen , Chen Yunqi 
Published: June 2023

PurposeOwing to dual constraints including liability of foreignness and liability of origin when emerging multinationals internationalize, they inevitably face the challenge of overseas legitimation. However, few studies have explored how latecomers cross the threshold of legitimacy in the dynamic context of transnational operation. The purpose of this paper is to unravel the evolution process, triggers and specific strategies of overseas legitimacy threshold crossing of emerging multinationals.Design/methodology/approachThrough the longitudinal case study of Haier Group and Goldwind Sci & Tech Co., Ltd, this study investigates the periodical characteristics of overseas legitimacy threshold crossings and the co-evolution among critical factors influencing the legitimation process in the host country.FindingsFirst, it summarizes that the legitimacy threshold in the host country experiences a sequential process from pragmatic legitimacy to normative legitimacy, and finally cognitive legitimacy. It is an inevitable choice for emerging multinational enterprises to realize and sustain legitimation from passive adaptation to active creation. Second, it reveals that the triggers for crossing the threshold of overseas legitimacy include periodically dynamic factors – international network linkage and resource system reconfiguration, as well as cross-stage spiral interaction effects. Third, it determines the specific strategies for crossing the threshold of overseas legitimacy, namely, replacement, upgrading and reconstruction of organizational identity, and reveals the important role of insisting on the country-of-origin Facebook in promoting the legitimation.Research limitations/implicationsThis study enriches the legitimacy threshold crossing literature from an evolutional perspective, especially the traditional static legitimacy research. This study also reveals the key impacting factors – international network linkage and resource system reconfiguration – and their evolution process interacted with the legitimation process.Practical implicationsThe emerging multinationals should break the stereotypes from developed markets in that only creating new cognitive patterns through active legitimate strategies can they truly cross the legitimacy threshold in the host country. The emerging multinationals also need to retain their own home country legitimacy traits – Facebook and balance the relation between the image of the home country and the image of host country.Originality/valueThis paper investigates the process of overseas legitimacy threshold crossing for emerging multinationals in a dynamic context of transnational operation, particularly with respect to the evolutionary role played by international network linkage and resource system reconfiguration.
Electric vehicles in the business processes and
sustainable development
Authors: Nanjundaswamy Abhishek , Kulal Abhinandan , Dinesh Sahana , Divyashree M.S. 
Published: June 2023

Purpose
The study aimed at analyzing operations managers’ perception of the
use of electric vehicles (EVs) in business processes and its impact on
overall business process cost (BPC) and sustainable development (SD).

Design/methodology/approach
The present study adopts the triangulation approach which is a combination of
quantitative and qualitative methods. The data was collected using
structured and scientifically tested questionnaires from the industrial
managers working in the industries in the Mysore region of Karnataka.
Descriptive statistics, factor analysis and structural equation models were
employed to analyze and interpret the data.

Findings
The findings revealed that the usage of EVs in Business Processes
significantly impacts the BPC (b = 0.851,
t = 8.037,
p < 0.01) and it is also the usage of EVs
in business processes can significantly impact SD
(b = 0.889,
t = 7.923,
p < 0.01). Thus, the adoption of EVs in
the business process offers many benefits to business organizations such as
minimized operational costs, an eco-friendly business model, more tax
incentives, less BPCs, a low-emission footprint and a contribution towards
SD at large.

Practical implications
Many business organizations operating in the present time show interest in
employing EVs in their business processes. Hence, before introducing EVs in
industries on a large scale, it becomes imperative to obtain the perception
of industrial managers who have already experienced its impact. This study
may help industrial organizations to understand the impact of EV on various
aspects of the business and to design a business model which would help in
achieving SD goals.

Originality/value
The use of EVs in the daily life of human beings and business activities is
gaining importance because of the various positive impacts. Therefore, it is
necessary to understand industrial managers’ opinions regarding the
use of EV in business activities.