bluuebunny/crossref_metadata_2025_split huggingface dataset:
Nanopore Sequencing of Short dsDNA after Elongation by Combination of Ligation and PEAR
Authors: Cheng Bingxiao , Hu Kunling , Song Ziting , An Ran , Liang Xingguo 
Published: July 2023

Abstract
Nanopore sequencing technology, as a third-generation method for DNA sequencing at the single-molecule level, has attracted much attention and developed quickly due to the advantages of low cost and ultra-long read. However, nanopore sequencing of short DNAs (&lt;500 bp) is not suitable due to the unfavorable cost performance and complicated data analysis. Here, we describe a novel method for nanopore sequencing using short dsDNA elongation by ligation and PEAR (SELP). Before sequencing, short PCR products are subjected to intermolecular ligation and subsequent elongation using PEAR (Polymerase-Endonuclease Amplification Reaction). The obtained long concatemers (thousands of base pairs) of repetitive DNA sequences are ideal samples for nanopore sequencing. The sequencing results demonstrate that short dsDNA can be elongated by more than 20 fold, and precise sequence analysis can be obtained through a single read. Accordingly, SELP-Seq can be used for simultaneous sequencing of multiple (even thousands of) short dsDNAs. Obviously, our approach can greatly expand the applications of nanopore sequencing, such as SNP analysis and high-throughput DNA detection.
Regression of nonalcoholic fatty liver disease is associated with reduced risk of incident diabetes: A longitudinal cohort study
Authors: Sinn Dong Hyun , Kang Danbee , Guallar Eliseo , Choi Sung Chul , Hong Yun Soo , Park Yewan , Cho Juhee , Gwak Geum-Youn 
Published: July 2023

Objective
Non-alcoholic fatty liver disease (NAFLD) is potentially reversible. However, whether improvement of NAFLD leads to clinical benefits remains uncertain. We investigated the association between regression of NAFLD and the risk of incident diabetes in a longitudinal way.

Methods
A cohort of 11,260 adults who had NAFLD at in an initial exam, had the second evaluation for NAFLD status at 1~2 years from an initial exam were followed up for incident diabetes from 2001 and 2016. NAFLD was diagnosed with abdominal ultrasound.

Results
At baseline, NAFLD was regressed in 2,559 participants (22.7%). During 51,388 person-years of follow-up (median 4 years), 1,768 participants developed diabetes. The fully adjusted hazard ratio (HR) for incident diabetes in participants with regressed NAFLD compared to those with persistent NAFLD was 0.81 [95% confidence interval (CI) 0.72–0.92]. When assessed by NAFLD severity, among participants with a low NAFLD fibrosis score (NFS) (< -1.455), participants with regressed NAFLD had a lower risk of incident diabetes than those with persistent NAFLD (HR 0.77, 95% CI 0.68–0.88). However, in participants with an intermediate to high NFS (≥ -1.455), the risk of incident diabetes was not different between NAFLD regression and persistence groups (HR 1.12, 95% CI 0.82–1.51).

Conclusions
Regression of NAFLD was associated with decreased risk of incident diabetes compared to persistent NAFLD. However, the benefit was evident only for NAFLD patients with low NFS. This suggests that early intervention for NAFLD, before advanced fibrosis is present, may maximize the metabolic benefit from NAFLD regression.
Two Pediatric Cases Within a Familial Cluster and Hereditary Diffuse Gastric Carcinoma: A Tale of 2 Sisters
Authors: Falgout Lacey , Gensler Lawrence L. 
Published: July 2023

ABSTRACT
Hereditary diffuse gastric cancer is a familial form of poorly differentiated signet ring cell carcinoma (SRCC) caused by a mutation in the CDH1/E-cadherin gene-mediating cell adhesion. CDH1 mutations are inherited in an autosomal dominant fashion and exhibit a high level of penetrance. SRCC diagnosis is exceedingly rare in the pediatric population. We report a case of SRCC in 2 sisters (10 and 15 years) and their father (41 years).
Prevalence, antibiotic susceptibility and plasmid profile of bacteria isolated from door handles of washrooms of a hospital in Bhopal, Madhya Pradesh
Authors: Shrivastava Shipra , Khare Naveen 
Published: July 2023

This study was conducted to determine the bacterial contamination in door handles of washrooms of a hospital, to determine the prevalence and antibiotic susceptibility pattern of the isolates. Plasmid profile was done to observe the presence or absence of plasmid among isolated bacteria from door handles of washrooms of a hospital.Washrooms of four different wards of a hospital were included in this study. Manual of Systematic Bacteriology and antibiotic susceptibility test was done by Kirby-Bauer method. Plasmid extraction was done according to modified hot alkaline method and staining technique and biochemixcal testing.Out of the 16 samples processed, 16 (100%) of them showed bacterial growth. A total of forty three (43) isolates were obtained. The bacteria isolated were spp. (37.21%), spp.(18.6%), .(16.28%), Fecal Coliform (13.95%), Micrococcus spp.(6.98%), spp.(4.65%), spp.(2.33%).Plasmid profiling revealed (11 out of 43) bacterial isolates contained 1 or more plasmids with different profiles.. Among the 43 isolates, 83.72% were found resistant to more than two antibiotics. Highest resistance percentage of the isolates was observed against Penicillin G (95.35%) followed by SXT (74.42%) and amoxicillin (65.12%), rifampicin (55.81%), tetracycline (18.60%), ciprofloxacin (23.26%), chloramphenicol (4.65%), gentamycin, (2.33%) and streptomycin (6.98%).Findings of this study indicate the presence of bacterial strains resistant to more than two antibiotics in door handles of washrooms of a hospital which can serve as potential source of diseases.
Seminario de Actualización de Producción Bovina 2017
Authors: Aponte Pedro , Ponce Christian , Salas Fernando 
Published: July 2023

SEMINARIO DE ACTUALIZACIÓN DE PRODUCCIÓN BOVINA 2017
La Escuela de Medicina Veterinaria de la Universidad San Francisco de Quito consciente de la situación ganadera de bovinos de leche del país, está orgullosa de presentar un Seminario de Actualización de Producción Bovina dirigido a productores de bovinos de leche, Médicos Veterinarios, Profesionales del área y estudiantes.
El seminario va a ser un éxito debido a que se va a obtener información científica aplicada en las áreas de Administración, Nutrición, Sanidad, y Reproducción con Expositores de alta calidad y experiencia. Adicionalmente, el seminario va a estar en un ambiente fraterno donde aparte de obtener información relevante, va a tener la capacidad de tener contacto con otros profesionales del área, realizar contactos de negocios y ponerse al día con amigos.
New Atlantis, Castalia, Thelema Abbey
Authors: Mezhuev B.V. 
Published: July 2023

The text gives a brief description of the history of the generation of intellectuals, which is commonly called the generation of the nineties. The author reflects on the path of this generation, choosing for analysis the fate of a small company of his classmates, those who at the moment have passed the fifty-year mark and have probably reached the acme of social maturity. It is emphasized that this generation has achieved great results in philosophical activity. The author notes the reason for his own alienation from the path of his generation and finds it in an internal protest against the tendency characteristic of his classmates to intellectually withdraw themselves from their time in order to be placed in a different context – temporal or spatial. It is noted that this very aspiration was borrowed by representatives of the generation of the nineties from the philosophical leaders of the sixties. The problem of the philosophical generation is considered in the context of the centuries-old search of the intellectual class for its place in society in obvious conflict with the social hierarchies existing in the traditional agrarian-class world.
An electromagnetic vibro-impact nonlinear energy sink for simultaneous vibration suppression and energy harvesting in vortex-induced vibrations
Authors: Li Haiqin , Li Shaohua , Ding Qian , Xiong Huai , Kong Xianren 
Published: July 2023

Abstract
An electromagnetic vibro-impact nonlinear energy sink (EM-VINES) is proposed in the application of vortex-induced vibration (VIV), for both purpose of vibration suppression and energy harvesting. The considered system consists of a cylinder-like bluff body subject to an oncoming flow, coupled to a magnet attachment moving in coil of gap enclosure. The fluid-structure interaction is treated using the classical Van der Pol oscillator model, and the non-smooth dynamics is formulated in a measure differential complementarity problem adapted with a Moreau-Jean time integration scheme. Comprehensive analyses are then conducted concerning the targeted energy transfer mechanism, as well as the internal competition of the energy flow. A performance indicator is defined over the lock-in region, to obtain the optimal balance between vibration suppression and energy harvesting. It is found that when the system is working in a strongly modulated regime with less than 2 impacts per cycle, a fast-scale targeted energy transfer could be activated over the whole lock-in region, making the EM-VINES behave efficiently for both vibration suppression and energy harvesting in the application of vortex-induced vibrations.
Magnetic resonance imaging of white matter response to diesel exhaust particles
Authors: Chakhoyan Ararat , Shkirkova Kristina , Sizdahkhani Saman , Huuskonen Mikko T. , Lamorie-Foote Krista , Diaz Arnold , Chen Selena , Liu Qinghai , D’Agostino Carla , Zhang Hongqiao , Mack Wendy J. , Sioutas Constantinos , Finch Caleb E. , Zlokovic Berislav , Mack William J. 
Published: July 2023

Abstract
Air pollution is associated with risks of dementia and accelerated cognitive decline. Rodent air pollution models have shown white matter vulnerability. This study uses diffusion tensor imaging (DTI) to quantify changes to white matter microstructure and tractography in multiple myelinated regions after exposure to diesel exhaust particulate (DEP). Adult C57BL/6 male mice were exposed to re-aerosolized DEP (NIST SRM 2975) at a concentration of 100 ug/m3 for 200 hours. Ex-vivo MRI analysis and fractional anisotropy (FA)-aided white matter tractography were conducted to study the effect of DEP exposure on the brain white matter tracts. Immunohistochemistry was used to assess myelin and axonal structure. DEP exposure for 8 weeks altered myelin composition in multiple regions. Diffusion tensor imaging (DTI) showed decreased FA in the corpus callosum (30%), external capsule (15%), internal capsule (15%), and cingulum (31%). Separate immunohistochemistry analyses confirmed prior findings. Myelin basic protein (MBP) was decreased (corpus callosum: 28%, external capsule: 29%), and degraded MPB increased (corpus callosum: 32%, external capsule: 53%) in the DEP group. White matter is highly susceptible to chronic DEP exposure. This study demonstrates the utility of DTI as a neuroanatomical tool in the context of air pollution and white matter myelin vulnerability.
Static Magnetic Field reduces cisplatin resistance via increasing apoptosis pathways and genotoxicity in cancer cell lines
Authors: Zafari Jaber , Rastegar-Pouyani Nima , Jouni Fatemeh Javani , Najjar Nabaa , Azarshin Seyedeh Zohreh , Jafarzadeh Emad , Abdolmaleki Parviz , Shirazi Farshad Hoseini Hoseini 
Published: July 2023

Abstract
Cisplatin is a chemotherapy drug widely used in cancer treatment. Alongside its clinical benefits, however, it may inflict intolerable toxicity and other adverse effects on healthy tissues. Due to the limitation of administering a high dose of cisplatin as well as cancer drug resistance, it is necessary to utilize new methods optimizing treatment modalities through both higher therapeutic efficacy and reduced administered doses of radiation and drugs. In this study, sensitive (A2780) and resistant (A2780CP) ovarian carcinoma cells underwent treatment with cisplatin + static magnetic field (SMF). First, the levels of genotoxicity after treatment were evaluated by Comet assay. Then, cell cycle analysis and apoptosis assay were conducted by a flow cytometer. Lastly, the expression levels of genes involved in apoptosis and cellular drug uptake were investigated by PCR. After treating different groups of cells for 24, 48, and 96 h, the co-treatment of SMF and drug as a combination managed to increase the amount of DNA damage in both sensitive and resistant cell lines. A considerable increase in mortality of cells was also observed mostly in the form of apoptosis, which was caused by inhibition of the cell cycle. The combination also increased the expression levels of apoptotic genes, namely P53 and P21; however, it did not have much effect on the expression levels of BCL2. Besides, the levels of CTR1 gene expression increased significantly in the groups receiving the aforementioned combination. Our study suggests that the combination of cisplatin + SMF might have clinical potential which needs further investigations through future studies.
Early Hip Fracture Surgery in Patients taking Direct Anticoagulants improves Outcome.
Authors: Devlieger Benjamin K. , Rommens Pol M. , Baranowski Andreas , Wagner Daniel 
Published: July 2023

Abstract
The increasing numbers of already endemic hip fractures in the elderly taking anticoagulants is a growing concern for daily surgical practice. Ample evidence demonstrates decreased morbidity and mortality in the general population when surgery is performed at the earliest possibility. Direct anticoagulants are relatively new drugs that can cause increased perioperative bleeding. Current guidelines propose stopping the drug to allow for elimination before performing elective surgery. Optimal management in urgent hip surgery is presently based on expert opinion with arbitrary cut-offs. In this study, we investigated whether patients taking direct anticoagulants would benefit from early surgical treatment, regardless of the timing since last intake. 340 Patients were included in the analysis, of which 59 took direct anticoagulants. The primary outcomes were time to surgery, postoperative transfusion rate, postoperative hemoglobin decrease, length of postoperative in-hospital stay (LOPS), revision rate and complication rate (medical and surgical). Our findings showed that the anticoagulated group was fit for discharge earlier when operated on within 24 hours. (p = 0.0167). Postoperative transfusion and medical complication rate tended to be lower when the operation was performed earlier. Revision rate due to hematomas were higher in the direct anticoagulant group without a relationship to time to surgery. Simple linear regression could not determine a relationship between postoperative hemoglobin change and time to surgery. We suggest that directly anticoagulated patients needing hip fracture surgery must be considered for early surgery regardless of last intake.
Computing with Heat Using Biocompatible Mott Neurons
Authors: Kim Kyung Min , Kim Gwangmin , In Jae Hyun , Lee Younghyun , Rhee Hakseung , Park Woojoon , Song Hanchan , Park Juseong , Jeon Jae Bum , Brown Timothy , Talin Albert , Kumar Suhas 
Published: July 2023

Abstract

Heat dissipation is a natural consequence of operating any electronic system. In nearly all of computing, such heat is minimized by design and discarded via cooling, while some post-digital electronics (such as phase-change memories) utilize only the static electrical outcome of such heat dissipation within a single device to represent information. Thus, neither the naturally produced heat energy nor its dynamics are directly used for computing. Here we demonstrate electronic NbOx Mott neurons integrated on a biocompatible flexible organic substrate, designed to exploit both the thermal dynamics of the Mott transition and the dynamics of thermal interactions with the organic substrate, to exhibit 18 bio-mimetic neuronal behaviors and frequency-based nociception (sensing of noxious stimuli) within a single component. Further, multiple Mott neurons can spatiotemporally communicate purely via their thermal dynamics, which we use for graph optimization by consuming over 107× lower energy compared to the best digital processors. Thus, we show that naturally produced heat in electronic systems and its dynamics can be used directly to encode and process information, thereby enabling a higher-complexity energy efficient and radically novel mixed-physics computing primitive.
Large-Scale Response-Aware Online ANN Search in Dynamic Datasets
Authors: Andrade Guilherme , Barreiros Willian , Rocha Leonardo , Ferreira Renato , Teodoro George 
Published: July 2023

Abstract

Similarity search is an key operation in Content-based multimedia retrieval(CBMR) applications. Online CBMR applications, which is the focus of thiswork, have to search in large and dynamic datasets that are updated during theexecution while offering low response times. Additionally, these applications aresubmitted to workloads that vary at runtime. The computing demands in thisscenario exceeds the processing power of a single computer, motivating the large-scale machines in the domain. Thus, in this work, we proposed a distributedmemory parallelization of similarity search that addresses the mentioned chal-lenges. Our solution employs the efficient Inverted File System with AsymmetricDistance Computation algorithm (IVFADC) algorithm as the baseline, which isextended here to support dynamic datasets. Further, we developed a dynamicresource management algorithm, called Multi-Stream Adaptation (MS-ADAPT),that is executed at run-time to change the computing resource assignment withthe goal of minimizing response times. We have evaluated our system solutionwith multiple data partitioning strategies using up to 160 compute nodes anda dataset with 344 billion multimedia descriptors. It demonstrated superlinearscalability for our Spatial-Aware data partition algorithms and MS-ADAPT out-performed the best static approach (oracle) by reducing the response times upto 32× on high-load cases.
An innovative concept mapping of the empowerment construct in dialogue with older people.
Authors: Noordink Thomas , Verharen Lisbeth , Schalk René , Regenmortel Tine Van 
Published: July 2023

Abstract

Empowerment has a central position in social work and services for older people. It gives meaning and provides a framework for a value orientation in which people gain or maintain control over decisions and actions affecting their health in relation to their process of aging. Measurements in this regard help to understand how empowerment manifests and comes about in social work. Operationalizing empowerment from the perspective of the target population is important to align with context specific interpretations of what empowerment means. This study aims at operationalizing psychological empowerment from the perspective of older persons in a systematic manner and in a way that gives voice to and creates opportunities for an empowering experience for these older persons. A design was chosen that is based on the concept mapping method, though tailored to accommodate the specific principles we assume in empowerment research and to align with target specific conditions that come to play with older persons. Three group sessions were conducted and a total of 28 older people participated. The results show an operationalisation of empowerment from the perspective of older people, with 58 statements divided over four components of empowerment; emotional, cognitive, relational and behavioural component. The context specific conceptualisation and operationalisation of empowerment that submerges, offers a helpful tool for social workers and the chosen methods seems to allow research participants to have empowering experiences by contributing.
Differential Temporal-Locality Adaptive Neural Networks (DTL-ANN): Bridging Cognitive Science with Machine Learning
Authors: Farshi A 
Published: July 2023

Abstract
Artificial Neural Networks (ANNs) have seen remarkable advancements, yet the pursuit of models capable of adaptive processing in both temporal and spatial domains is still an ongoing challenge. We propose a groundbreaking architecture, Differential Temporal-Locality Adaptive Neural Networks (DTL-ANN), that embodies the adaptability and locality of processing characteristic of the human brain. Through the integration of a dynamically adaptable temporal context window and spatial receptive field, DTL-ANN learns to adapt these parameters over time, utilizing a feedback mechanism from the output layer. The novel model surpasses conventional benchmarks across a multitude of tasks, spanning natural language processing, computer vision, and signal processing. Significantly, DTL-ANN exhibits a robust resilience to shifting data patterns, demonstrating an adaptability akin to biological neural systems and marking a critical step forward in the realm of machine learning and artificial intelligence.
Vegetative Growth of Paprika Plant (Capsicum annum var grossum) at Three Diferent Sound Frequencies
Authors: Meydiyawati Ega , Linda Riza , Ifadatin Sitti 
Published: July 2023

Paprika (Capsicum annum var grossum) is a horticultural commodity with high economic value. Cultivation of paprika is currently still relatively low, for West Kalimantan. This research was conducted to see the effect of exposure to different sound frequencies on the growth of Paprika (Capsicum annum var. grossum) plants. The time for the study was from May 16 2022 to August 14 2022. This study used a Completely Randomized Design (CRD) which had 4 different treatments namely control, 396 hz sound exposure, 528 hz sound exposure, and 660 hz sound exposure. Data were analyzed using one way ANOVA followed by Duncan's test with a certainty level of 0.05%. The results showed that exposure to 528 sound gave the best results on plant height, leaf area, pore length, pore width, chlorophyll B, and total chlorophyll. In conclusion, that exposure to sound can increase the growth of pepper plants.
The Effect of Product Quality, Price and Quality of Service on Consumer Satisfaction (Case study in Perumahan Sawangan Indah Depok)
Authors: Haryantini Haryantini 
Published: July 2023

This study aims to determine the effect of product quality, price and service quality on MyRepublic consumer satisfaction in Serua Permai Housing, South Tangerang. This research method is quantitative method. The population in this study were 243 users with a sample of 151 respondents. The analytical model used is Multiple Linear Regression which is processed using the SPSS version 26 application program, data is collected using questionnaires. Based on the results of this research, it shows that product quality has a significant effect on consumer satisfaction. Price has a significant effect on Consumer Satisfaction. Service Quality has a significant effect on consumer satisfaction and and simultaneously Product Quality, Price and Service Quality has significant effect on MyRepublic Consumer Satisfaction in Serua Permai, South Tangerang.