bluuebunny/crossref_metadata_2025_split huggingface dataset:
Primed for change: The effect of a blood prime on peripheral blood stem cell collection and accuracy of a prediction tool in pediatric patients
Authors: Rahim Mahvish Q. , Goebel W. Scott , Delph John , Soundar Esther 
Published: May 2023

AbstractPediatric apheresis collection of peripheral blood stem cells for autologous transplantation often requires use of a blood prime. We evaluated the relationship between pre‐apheresis blood CD34+ counts and final CD34+ yield with use of a blood prime. Forty patients underwent apheresis stem cell collection in a 5 year period in our hospital, of which 27 required blood priming of the apheresis machine. Despite the blood prime group having significantly higher pre‐apheresis CD34+ cell counts, this group processed a relatively higher volume of blood due to a higher dilutional effect and collected significantly less than predicted CD34+ cell yield. Use of weight‐specific collection efficiencies and dilution‐adjusted pre‐apheresis CD34+ counts will help in accurately estimating the whole blood volume to process for PBSC collection and therefore increase efficiency and decrease the overall cost of collection.
Stability of glycated haemoglobin (HbA1c) measurements from whole blood samples kept at −196°C for seven to eight years in The Malaysian Cohort study
Authors: Abdullah Noraidatulakma , Goh Ying‐Xian , Othman Raihannah , Ismail Norliza , Jalal Nazihah , Wan Sallam Wan Ahmad Faisal , Husin Nurul Faeizah , Shafie Ahmad Syafiq , Awang Afifah , Kamaruddin Mohd Arman , Jamal Rahman 
Published: May 2023

AbstractObjectiveGlycated haemoglobin (HbA1c) is a standard indication for screening type 2 diabetes that also has been widely used in large‐scale epidemiological studies. However, its long‐term quality (in terms of reproducibility) stored in liquid nitrogen is still unknown. This study is aimed to evaluate the stability and reproducibility of HbA1c measurements from frozen whole blood samples kept at −196°C for more than 7 years.MethodsA total of 401 whole blood samples with a fresh HbA1c measurement were randomly selected from The Malaysian Cohort's (TMC) biobank. The HbA1c measurements of fresh and frozen (stored for 7–8 years) samples were assayed using different high‐performance liquid chromatography (HPLC) systems. The HbA1c values of the fresh samples were then calculated and corrected according to the later system. The reproducibility of HbA1c measurements between calculated‐fresh and frozen samples was assessed using a Passing‐Bablok linear regression model. The Bland–Altman plot was then used to evaluate the concordance of HbA1c values.ResultsThe different HPLC systems highly correlated (r = 0.99) and agreed (ICC = 0.96) with each other. Furthermore, the HbA1c measurements for frozen samples strongly correlate with the corrected HbA1c values of the fresh samples (r = 0.875) with a mean difference of −0.02 (SD: −0.38 to 0.38). Although the mean difference is small, discrepancies were observed within the diabetic and non‐diabetic samples.ConclusionThese data demonstrate that the HbA1c measurements between fresh and frozen samples are highly correlated and reproducible.
Is there evidence of a causal link between childhood maltreatment and attention deficit/hyperactivity disorder? A systematic review of prospective longitudinal studies using the Bradford‐Hill criteria
Authors: Bali Paraskevi , Sonuga‐Barke Edmund , Mohr‐Jensen Christina , Demontis Ditte , Minnis Helen 
Published: May 2023

AbstractBackgroundStudies report an elevated risk of maltreatment in children with attention deficit/hyperactivity disorder (ADHD), and elevated levels of ADHD in people who suffered childhood maltreatment (CM). However, the direction(s) of causality between CM and ADHD remain unclear—does ADHD create a context for CM, does CM cause ADHD, or both?ObjectiveThis study systematically reviews and qualitatively synthesizes the research evidence relating to this question using Bradford‐Hill criteria for establishing causality—strength, temporality, dose‐response and plausibility.MethodsWe conducted a systematic review, following PRISMA guidelines, of prospective longitudinal studies examining both CM and ADHD. We then used Bradford‐Hill criteria to assess the quality of evidence for a causal link between CM and ADHD.ResultsAll 11 included studies demonstrated an association between CM and ADHD. Seven included evidence for temporality: five suggesting that CM precedes ADHD in the lifespan; two suggesting ADHD precedes CM. Four studies demonstrated a dose response relationship in which greater CM exposure was associated with elevated risk of ADHD. Studies presented a range of plausible mechanisms, including CM causing ADHD through biological programming, versus ADHD causing CM through parental stress.ConclusionsThe high quality prospective longitudinal studies reviewed confirm the association between ADHD and CM, but present conflicting evidence about the direction of causality and mechanisms underpinning this association. To better understand the complex interplay between CM and ADHD, more studies using new research designs will be required that can partition effects by type of CM and account for bidirectional effects and other complexities.
Successful endoscopic submucosal dissection of colorectal lipoma with an overlying adenoma
Authors: Furuta Akito , Omoto Shunsuke , Inoue Taro , Yanai Mitsuru , Iwabe Hideo , Takihara Hiroshi , Ishibashi Kohei , Tanaka Hironori , Matsuura Ko , Ogata Shunsuke , Yokomura Akitaka , Hoshikawa Masato , Kono Michihito , Koriyama Takasi , Tazawa Tomohiko , Tsuyuguchi Eri , Yamasaki Yasuo , Esumi Shun , Tsuruta Yoshimasa , Shishimoto Takahiro , Yamamoto Masaki , Ono Wataru 
Published: May 2023

AbstractWe report the case of a 65‐year‐old woman whose colonoscopy revealed a soft submucosal tumor approximately 7 cm in diameter in the ascending colon with an overlying flat lesion. The tumor was diagnosed as a lipoma with an overlying adenoma. Endoscopic submucosal dissection (ESD) was performed. Pathological examination revealed that the epithelium was a low‐grade tubulovillous adenoma, while the submucosal yellow tumor was a lipoma. ESD appears to be a safe and effective treatment for colorectal lipomas overlying lipomas with colorectal adenomas.
Hemoglobin‐platelet index as a prognostic factor in patients with peripheral T‐cell lymphoma
Authors: Yagi Yu , Kanemasa Yusuke , Sasaki Yuki , Okumura Shunichi , Watanabe Takako , Ishimine Kento , Hayashi Yudai , Mino Mano , Ohigashi An , Morita Yuka , Tamura Taichi , Nakamura Shohei , Okuya Toshihiro , Shimoyama Tatsu 
Published: May 2023

AbstractPeripheral T‐cell lymphoma (PTCL) is a heterogeneous group of aggressive lymphomas with a poor prognosis. The International Prognostic Index (IPI) and the Prognostic Index for PTCL‐unspecified (PIT) is used to predict the prognosis of PTCL. The hemoglobin‐platelet index (HPI), based on anemia and thrombocytopenia status, is associated with the prognosis of diffuse large B‐cell lymphoma. However, its significance in terms of predicting the prognosis of PTCL has not been fully investigated. We herein retrospectively analyzed 100 patients with newly diagnosed PTCL in our department. At a median follow‐up of 3.2 years, the median progression‐free survival (PFS) and overall survival (OS) was 0.72 (95% confidence interval [CI]: 0.56–1.2) years and 2.0 (95% CI: 1.5–4.7) years, respectively. Multivariate analysis revealed that elevated lactic dehydrogenase (LDH) and hypoalbuminemia were independent adverse variables for PFS. The HPI showed significant predictive value for both PFS and OS. As a new prognostic model comprising the HPI, LDH, and albumin, the LA‐HPI allowed the stratification of patients into four distinct risk subgroups: low risk (zero risk factors), low‐intermediate risk (one risk factors), high‐intermediate risk (two or three risk factors), or high risk (four risk factors). The PFS and OS differed significantly among the patients by the LA‐HPI score. The LA‐HPI demonstrated better predictive performance compared to the IPI, PIT, and HPI. Our data demonstrated the prognostic utility of the HPI in patients with PTCL. The LA‐HPI, incorporating four readily obtainable parameters, exhibited superior performance compared to traditional indices.
Biomarkers to predict disease progression and therapeutic response in isolated methylmalonic acidemia
Authors: Manoli Irini , Gebremariam Abigael , McCoy Samantha , Pass Alexandra R. , Gagné Jack , Hall Camryn , Ferry Susan , Van Ryzin Carol , Sloan Jennifer L. , Sacchetti Elisa , Catesini Giulio , Rizzo Cristiano , Martinelli Diego , Spada Marco , Dionisi‐Vici Carlo , Venditti Charles P. 
Published: May 2023

AbstractMethylmalonic Acidemia (MMA) is a heterogenous group of inborn errors of metabolism caused by a defect in the methylmalonyl‐CoA mutase (MMUT) enzyme or the synthesis and transport of its cofactor, 5′‐deoxy‐adenosylcobalamin. It is characterized by life‐threatening episodes of ketoacidosis, chronic kidney disease, and other multiorgan complications. Liver transplantation can improve patient stability and survival and thus provides clinical and biochemical benchmarks for the development of hepatocyte‐targeted genomic therapies. Data are presented from a US natural history protocol that evaluated subjects with different types of MMA including mut‐type (N = 91), cblB‐type (15), and cblA‐type MMA (17), as well as from an Italian cohort of mut‐type (N = 19) and cblB‐type MMA (N = 2) subjects, including data before and after organ transplantation in both cohorts. Canonical metabolic markers, such as serum methylmalonic acid and propionylcarnitine, are variable and affected by dietary intake and renal function. We have therefore explored the use of the 1‐13C‐propionate oxidation breath test (POBT) to measure metabolic capacity and the changes in circulating proteins to assess mitochondrial dysfunction (fibroblast growth factor 21 [FGF21] and growth differentiation factor 15 [GDF15]) and kidney injury (lipocalin‐2 [LCN2]). Biomarker concentrations are higher in patients with the severe mut0‐type and cblB‐type MMA, correlate with a decreased POBT, and show a significant response postliver transplant. Additional circulating and imaging markers to assess disease burden are necessary to monitor disease progression. A combination of biomarkers reflecting disease severity and multisystem involvement will be needed to help stratify patients for clinical trials and assess the efficacy of new therapies for MMA.
Prognostic nutritional score based on pretreatment lymphocyte, platelet, and prealbumin predicts prognosis in patients with pancreatic cancer
Authors: Liang Yuexiang , Guo Hanhan , Man Quan , Chang Shaofei , Wang Erpeng , Gao Song 
Published: May 2023

AbstractBackground and ObjectivesPretreatment immunological indicators and nutritional factors are associated with survival of many malignancies. This study aims to develop a prognostic nutritional score based on a combination of pretreatment lymphocyte, platelet, and prealbumin (Co‐LPPa) in patients with pancreatic cancer (PC) and to investigate the prognostic significance of this score.MethodsPatients who underwent pancreatectomy with a curative intent for PC were retrospectively enrolled. A pretreatment prognostic score was established by immunological indicators and nutritional factors that were independently associated with survival.ResultsPretreatment lymphocyte (<1.6 × 109/L), platelet (<160 × 109/L) and prealbumin (<0.23 g/L) were independently associated with poorer overall survival (OS) and recurrence‐free survival (RFS), and were used to create the Co‐LPPa score. The Co‐LPPa scores were inversely related to OS and RFS, and were able to stratify survival into four groups. The survival differences among the four groups were all significant. Besides, the Co‐LPPa scores could stratify survival independently of pathological prognostic factors. The Co‐LPPa score was superior to prognostic nutritional index and carbohydrate antigen 19‐9 in predicting OS and RFS.ConclusionThe Co‐LPPa score could accurately predict the prognosis of PC patients who underwent curative resection. The score may be helpful for preoperative therapeutic strategies.
Light‐Based 3D Printing of Gelatin‐Based Biomaterial Inks to Create a Physiologically Relevant In Vitro Fish Intestinal Model
Authors: Szabó Anna , Pasquariello Rolando , Costa Pedro F. , Pavlovic Radmila , Geurs Indi , Dewettinck Koen , Vervaet Chris , Brevini Tiziana A. L. , Gandolfi Fulvio , Van Vlierberghe Sandra 
Published: May 2023

AbstractTo provide prominent accessibility of fishmeal to the European population, the currently available, time‐ and cost‐extensive feeding trials, which evaluate fish feed, should be replaced. The current paper reports on the development of a novel 3D culture platform, mimicking the microenvironment of the intestinal mucosa in vitro. The key requirements of the model include sufficient permeability for nutrients and medium‐size marker molecules (equilibrium within 24 h), suitable mechanical properties (G' < 10 kPa), and close morphological similarity to the intestinal architecture. To enable processability with light‐based 3D printing, a gelatin‐methacryloyl‐aminoethyl‐methacrylate‐based biomaterial ink is developed and combined with Tween 20 as porogen to ensure sufficient permeability. To assess the permeability properties of the hydrogels, a static diffusion setup is utilized, indicating that the hydrogel constructs are permeable for a medium size marker molecule (FITC‐dextran 4 kg mol−1). Moreover, the mechanical evaluation through rheology evidence a physiologically relevant scaffold stiffness (G' = 4.83 ± 0.78 kPa). Digital light processing‐based 3D printing of porogen‐containing hydrogels results in the creation of constructs exhibiting a physiologically relevant microarchitecture as evidenced through cryo‐scanning electron microscopy. Finally, the combination of the scaffolds with a novel rainbow trout (Oncorhynchus mykiss) intestinal epithelial cell line (RTdi‐MI) evidence scaffold biocompatibility.
Simultaneous Construct Surface Microstructural and Internal Network‐Like Conductive Pathways of Poly(l‐lactic acid)/Carbon Nanomaterials Composite Foams
Authors: Sun Xin , Guo Yunfeng , Yang Shengdu , Wang Kai , Xie Lan , Xue Bai 
Published: May 2023

AbstractThe poly(l‐lactic acid) (PLLA)/carbon nanomaterials composite foams with hierarchical surface microstructural and internal conductive pathways are successfully prepared by a simple crystallization‐assisted rapid phase separation (CARPS). The dimension and morphology of carbon nanomaterials can induce different crystallization forms to construct the hierarchical surface microstructure, and they are distributed on the phase interface of solvent and non‐solvent to form conductive pathways. It is found that the heterogeneous nucleation of nanomaterials promotes a significant increase in crystallinity, and a stacked granular structure formed on the surface promotes the increase of the water contact angle to 148.7°. Foams with interconnecting pore structures contribute to the formation of 85.3% porosity and 12.33 g g−1 oil absorption. Carbon nanomaterials are distributed on the pore walls of the porous foam, which converts the foam from an insulating material to a conducting polymer. Furthermore, the uniform distribution of nanomaterials significantly affects the thermal stability of the PLLA. In belief, the multifunctional biodegradable foam, prepared by a CARPS method, makes it promising for industrial production and has potential applications in electrical conductivity, oil‐water separation, and many other fields.
Tough, Eu3+‐Induced Luminescent Hydrogel as Flexible Chemosensor for Real‐Time Quantitative Detection of Zn2+ Ion
Authors: Chen Liang , Cui Yuanzhi , Ruan Jiaping , Zhang Xincheng , Zhang Yifan , Rao Ping , Ren Wenshan 
Published: May 2023

AbstractHerein, a novel tough luminescent hydrogel with Europium is fabricated using a facile copolymerization process by introducing 2,2’:6’,2‐terpyridine (TPy) into a dual physical cross‐linked hydrogel. The obtained P(NAGA‐co‐MAAc)/Eu/TPy (x) (x refers to the feed ratio of NAGA to MAAc) hydrogels not only show outstanding mechanical performances (fracture strength, ≈2.5 MPa), but also give a special ability of rapid detection to low concentrations of zinc ions. Attractively, the theoretical limits of detection (LOD) of the hydrogel sensors are calculated as 1.6 µm, which is acceptable within the WHO limit. Furthermore, the continuous change in fluorescence of P(NAGA‐co‐MAAc)/Eu/TPy (10) strips upon contact with Zn2+ can be clearly observed by the naked eyes with the aid of a portable UV lamp, resulting in semi‐quantitative naked‐eyes detection through a standard colorimetric card. Moreover, by identifying the RGB value of the hydrogel sensor, it can also realize quantitative analysis. Therefore, excellence in sensing, simplicity in structure, and convenience in using make P(NAGA‐co‐MAAc)/Eu/TPy (10) hydrogel as a superior fluorescent chemosensor of Zn2+ ions.
Small molecules targeting different cellular pathologies for the treatment of amyotrophic lateral sclerosis
Authors: Elmansy Mohamed F. , Reidl Cory T. , Rahaman Mizzanoor , Özdinler P. Hande , Silverman Richard B. 
Published: May 2023

AbstractAmyotrophic lateral sclerosis (ALS) is a devastating neurodegenerative disease in which the motor neuron circuitry displays progressive degeneration, affecting mostly the motor neurons in the brain and in the spinal cord. There are no effective cures, albeit three drugs, riluzole, edaravone, and AMX0035 (a combination of sodium phenylbutyrate and taurursodiol), have been approved by the Food and Drug Administration, with limited improvement in patients. There is an urgent need to build better and more effective treatment strategies for ALS. Since the disease is very heterogenous, numerous approaches have been explored, such as targeting genetic mutations, decreasing oxidative stress and excitotoxicity, enhancing mitochondrial function and protein degradation mechanisms, and inhibiting neuroinflammation. In addition, various chemical libraries or previously identified drugs have been screened for potential repurposing in the treatment of ALS. Here, we review previous drug discovery efforts targeting a variety of cellular pathologies that occur from genetic mutations that cause ALS, such as mutations inSOD1, C9orf72, FUS, and TARDP‐43genes. These mutations result in protein aggregation, which causes neuronal degeneration. Compounds used to target cellular pathologies that stem from these mutations are discussed and comparisons among different preclinical models are presented. Because the drug discovery landscape for ALS and other motor neuron diseases is changing rapidly, we also offer recommendations for a novel, more effective, direction in ALS drug discovery that could accelerate translation of effective compounds from animals to patients.
Baseline predictors of fatigue and persistent fatigue in rheumatoid arthritis: A longitudinal observational study
Authors: Fazaa Alia , Boussaa Hiba , Ouenniche Kmar , Miladi Saoussen , Makhlouf Yasmine , Belhadj Salwa , Ben Abdelghani Kawther , Laatar Ahmed 
Published: May 2023

AbstractObjectivesTo determine factors associated with fatigue in patients with rheumatoid arthritis (RA), and to identify baseline predictors of persistent fatigue at 12 months of follow‐up.MethodsWe enroled patients with RA fulfiling the 2010 American College of Rheumatology/European League Against Rheumatism criteria. Fatigue was assessed using the Arabic version of the Functional Assessment of Chronic Illness Therapy—Fatigue (FACIT‐F). Using univariate and multivariate analyses, we examined baseline variables associated with fatigue and persistent fatigue (if the FACIT‐F score was less than 40 at baseline and 12 months of follow‐up).ResultsWe included 100 RA patients of whom 83% reported fatigue. At baseline, the FACIT‐F score was significantly associated with older age (p = 0.007), pain (p < 0.001), global patient assessment (GPA) (p < 0.001), tender joint count (TJC) (p < 0.001), swollen joint count (p = 0.003), erythrocyte sedimentation rate (ESR) (p < 0.001), disease activity score (DAS28 ESR) (p < 0.001), and health assessment questionnaire (HAQ) (p < 0.001). At 12 months of follow‐up, the percentage of patients who reported persistent fatigue was 60%. The FACIT‐F score was significantly associated with age (p = 0.015), symptom duration (p = 0.002), pain (p < 0.001), GPA (p < 0.001), TJC (p < 0.001), C‐Reactive Protein (p = 0.007), ESR (p = 0.009), DAS28 ESR (p < 0.001), and HAQ (p < 0.001). Pain was an independent baseline predictor of persistent fatigue (OR = 0.969 (95% CI [0.951–0.988]), p = 0.002).ConclusionsFatigue is a frequent symptom in RA. Pain, GPA, disease activity and disability were associated with fatigue and persistent fatigue. Baseline pain was the only independent predictor of persistent fatigue.
Differentiation between rebound thymic hyperplasia and thymic relapse after chemotherapy in pediatric Hodgkin lymphoma
Authors: Franke Friedrich Christian , Damek Adrian , Steglich Jonas , Kurch Lars , Hasenclever Dirk , Georgi Thomas W , Wohlgemuth Walther Alexander , Mauz‐Körholz Christine , Körholz Dieter , Kluge Regine , Landman‐Parker Judith , Wallace William Hamish , Fosså Alexander , Vordermark Dirk , Karlen Jonas , Fernández‐Teijeiro Ana , Cepelova Michaela , Klekawka Tomasz , Attarbaschi Andishe , Ceppi Francesco , Hraskova Andrea , Uyttebroeck Anne , Beishuizen Auke , Dieckmann Karin , Leblanc Thierry , Moellers Martin , Buerke Boris , Stoevesandt Dietrich 
Published: May 2023

AbstractBackgroundRebound thymic hyperplasia (RTH) is a common phenomenon caused by stress factors such as chemotherapy (CTX) or radiotherapy, with an incidence between 44% and 67.7% in pediatric lymphoma. Misinterpretation of RTH and thymic lymphoma relapse (LR) may lead to unnecessary diagnostic procedures including invasive biopsies or treatment intensification. The aim of this study was to identify parameters that differentiate between RTH and thymic LR in the anterior mediastinum.MethodsAfter completion of CTX, we analyzed computed tomographies (CTs) and magnetic resonance images (MRIs) of 291 patients with classical Hodgkin lymphoma (CHL) and adequate imaging available from the European Network for Pediatric Hodgkin lymphoma C1 trial. In all patients with biopsy‐proven LR, an additional fluorodeoxyglucose (FDG)‐positron emission tomography (PET)‐CT was assessed. Structure and morphologic configuration in addition to calcifications and presence of multiple masses in the thymic region and signs of extrathymic LR were evaluated.ResultsAfter CTX, a significant volume increase of new or growing masses in the thymic space occurred in 133 of 291 patients. Without biopsy, only 98 patients could be identified as RTH or LR. No single finding related to thymic regrowth allowed differentiation between RTH and LR. However, the vast majority of cases with thymic LR presented with additional increasing tumor masses (33/34). All RTH patients (64/64) presented with isolated thymic growth.ConclusionIsolated thymic LR is very uncommon. CHL relapse should be suspected when increasing tumor masses are present in distant sites outside of the thymic area. Conversely, if regrowth of lymphoma in other sites can be excluded, isolated thymic mass after CTX likely represents RTH.