bluuebunny/crossref_metadata_2025_split huggingface dataset:
A Stable and Efficient Genetic Transformation Method for Embryogenic Callus of Fraxinus mandshurica
Authors: An Yan , Dong Hao , Zhao Wenna , Shen Hailong , Yang Ling , Li Shujuan 
Published: May 2023

Fraxinus mandshurica is a widely used greening and ornamental tree species. However, its genetic transformation system has been hampered by problems such as low transformation efficiency, among others, which can hinder research related to molecular breeding and the analysis of functional genes. Thus, in this study, a novel genetic transformation method for efficient transformation of the embryonic callus of Fraxinus mandshurica was investigated. The method was optimized in terms of factors such as antibiotics, infection solution concentrations, co-culture time, and somatic embryo maturation. The results indicated that the optimal antibiotic concentration was 10 mg·L−1 of hygromycin (Hyg). At this point, the callus proliferation multiple was only 0.12. The highest transformation efficiency was found to be 93.93% when the absorbance of the infection solution concentration at OD600 was 0.4. Interestingly, transformation efficiency was found to be highest (77.9%) at 48 h of co-culture, with a GUS staining rate of 88.23%. The medium for somatic embryo maturation of transformed callus was half-strength MS medium (MS 1/2) containing 60 g·L−1 polyethylene glycol, 1 mg·L−1 abscisic acid, 400 mg·L−1 casein enzymatic hydrolysate (CH), 20 g·L−1 sucrose, 1 g·L−1 activated charcoal, and 5 g·L−1 gellan gum. The medium for somatic embryo germination was MS ½, containing 0.2 mg·L−1 of N-(Phenylmethyl)-9H-purin-6-amine(6-BA) and 5.0 mg·L−1 of gibberellin (GA). These results are of significance for the verification of the gene function and future genetic improvement of Fraxinus mandshurica.
Mixed-Species Acacia Plantation Decreases Soil Organic Carbon and Total Nitrogen Concentrations but Favors Species Regeneration and Tree Growth over Monoculture: A Thirty-Three-Year Field Experiment in Southern China
Authors: Ouyang Shengnan , Tie Liehua , Rao Xingquan , Cai Xi’an , Liu Suping , Vitali Valentina , Wei Lanying , Yu Qingshui , Sun Dan , Lin Yongbiao , Bose Arun K. , Gessler Arthur , Shen Weijun 
Published: May 2023

Mixed-species plantations of trees with N-fixing species have the potential of promoting forest productivity and soil fertility. However, few studies in the literature have addressed the advantages of mixed-species plantations of leguminous trees over monocultures of leguminous trees based on in situ inventories over a long time period. Here, we monitored the dynamics of tree community composition, vegetation biomass, soil nutrients, and soil microbial phospholipid fatty acids (PLFAs), in an Acacia mangium monoculture plantation during 33 years of development and compared it with a mixed-species plantation of A. mangium associated with 56 native species which were underplanted 14 years after the initial establishment. Leaf N and phosphorus (P) concentrations of three main species in the overstory and understory of the A. mangium monoculture were measured. Our results showed that the soil organic carbon (SOC), total nitrogen (TN), and available phosphorus (AP) concentrations significantly increased over time during the approximately thirty years of A. mangium monoculture plantation, while the disadvantages were associated with new species regeneration and the increment of vegetation biomass. In the A. mangium monoculture plantation, leaf N concentration of A. mangium,Rhodomyrtus tomentosa, and Dicranopteris dichotoma continuously increased from 21 to 31 years, while the leaf P concentration of A. mangium and R. tomentosa decreased. The mixed-species plantations of A. mangium with native tree species had lower SOC and soil TN concentrations, more new tree species recruitment in the understory, and faster vegetation biomass increment than the A. mangium monoculture. However, the PLFAs of soil microbial groups were slightly different between the two types of plantations. We conclude that improved soil N nutrient condition by A. mangium monoculture benefits N absorption by A. mangium, R. tomentosa, and D. tomentosa, while low soil AP limits P absorption by A. mangium and R. tomentosa. Meanwhile, transforming the A. mangium monoculture into a mixed-species plantation via the introduction of multiple native species into the A. mangium monoculture decreases SOC and TN concentrations but the advantages include improving forest regeneration and maintaining forest growth in a long-term sequence. These findings provide useful and practical suggestions for managing forest monocultures of A. mangium in subtropical regions.
Genomic and Transcriptional Profiles of Kelch-like (klhl) Gene Family in Polyploid Carassius Complex
Authors: Peng Fang , Zhou Li , Lu Weijia , Gan Ruihai , Lu Meng , Li Zhi , Zhang Xiaojuan , Wang Yang , Gui Jianfang 
Published: May 2023

Genome duplication supplies raw genetic materials and has been thought to be essential for evolutionary innovation and ecological adaptation. Here, we select Kelch-like (klhl) genes to study the evolution of the duplicated genes in the polyploid Carassius complex, including amphidiploid C. auratus and amphitriploid C. gibelio. Phylogenetic, chromosomal location and read coverage analyses indicate that most of Carassius klhl genes exhibit a 2:1 relationship with zebrafish orthologs and confirm two rounds of polyploidy, an allotetraploidy followed by an autotriploidy, occurred during Carassius evolution. The lineage-specific expansion and biased retention/loss of klhl genes are also found in Carassius. Transcriptome analyses across eight adult tissues and seven embryogenesis stages reveal varied expression dominance and divergence between the two species. The expression of klhls in response to Carassius herpesvirus 2 infection shows different expression changes corresponding to distinct herpesvirus resistances in three C. gibelio gynogenetic clones. Finally, we find that most C. gibelio klhl genes possess three alleles except eight genes that have lost one or two alleles due to genome rearrangement. The allele expression bias is prosperous for Cgklhl genes and varies during embryogenesis owning to the sequential expression manner of the alleles. The current study provides global insights into the genomic and transcriptional evolution of duplicated genes in a given superfamily resulting from multiple rounds of polyploidization.
Comparative Proteomic Analysis of the Venoms from the Most Dangerous Scorpions in Morocco: Androctonus mauritanicus and Buthus occitanus
Authors: Hilal Ines , Khourcha Soukaina , Safi Amal , Hmyene Abdelaziz , Asnawi Syafiq , Othman Iekhsan , Stöcklin Reto , Oukkache Naoual 
Published: May 2023

Morocco is known to harbor two of the world’s most dangerous scorpion species: the black Androctonus mauritanicus (Am) and the yellow Buthus occitanus (Bo), responsible for 83% and 14% of severe envenomation cases, respectively. Scorpion venom is a mixture of biological molecules of variable structures and activities, most of which are proteins of low molecular weights referred to as toxins. In addition to toxins, scorpion venoms also contain biogenic amines, polyamines, and enzymes. With the aim of investigating the composition of the Am and Bo venoms, we conducted an analysis of the venoms by mass spectrometry (ESI-MS) after separation by reversed-phase HPLC chromatography. Results from a total of 19 fractions obtained for the Am venom versus 22 fractions for the Bo venom allowed the identification of approximately 410 and 252 molecular masses, respectively. In both venoms, the most abundant toxins were found to range between 2–5 kDa and 6–8 kDa. This proteomic analysis not only allowed the drawing of an extensive mass fingerprint of the Androctonus mauritanicus and Buthus occitanus venoms but also provided a better insight into the nature of their toxins.
The Effects of Coriandrum sativum L. and Cucurbita pepo L. against Gastrointestinal Parasites in Swine: An In Vivo Study
Authors: Băieş Mihai-Horia , Cotuţiu Vlad-Dan , Spînu Marina , Mathe Attila , Cozma-Petruț Anamaria , Miere Doina , Bolboacǎ Sorana D. , Cozma Vasile 
Published: May 2023

Parasitic diseases are responsible for substantial losses in reproduction and productivity in swine, creating a major impairment to efficient and profitable livestock management. The use of phytotherapeutic remedies has notably increased over the past decade due to their bioavailability, decreased toxicity, non-polluting nature, and to some extent due to their antiparasitic effect. The aim of this study was to evaluate the antiparasitic potential of Cucurbita pepo L. and Coriandrum sativum L. against protozoa and nematodes found in swine. The samples were collected from weaners, fatteners, and sows and examined via flotation (Willis and McMaster), active sedimentation, Ziehl-Neelsen staining as modified by Henricksen, a modified Blagg method, and eggs/oocyst culture. The parasite species detected were Ascaris suum, Trichuris suis, Oesophagostomum spp., Balantioides coli (syn. Balantidium coli), Eimeria spp., and Cryptosporidium spp., depending on age category. A dose of 500 mg/kg bw/day of C. pepo and 170 mg/kg bw/day of C. sativum powders, administered for ten consecutive days, demonstrated a pronounced anthelmintic (pumpkin) and antiprotozoal (coriander) effect against the aforementioned parasites. Future studies are required to ascertain the optimal dose that maximizes their antiparasitic effectiveness. The current study represents the first Romanian report on the in vivo antiparasitic activity of these two plants tested on digestive parasites in swine.
Overview of the Lunar In Situ Resource Utilization Techniques for Future Lunar Missions
Authors: Zhang Peng , Dai Wei , Niu Ran , Zhang Guang , Liu Guanghui , Liu Xin , Bo Zheng , Wang Zhi , Zheng Haibo , Liu Chengbao , Yang Hanzhe , Bai Yifan , Zhang Yang , Yan Dong , Zhou Kefa , Gao Ming 
Published: May 2023

Along with the rapid development of space technology, extraterrestrial exploration has gradually tended to further-distanced and longer-termed planet exploration. As the first step of an attempt for humans to build a perpetual planet base, building a lunar base by in situ resource utilization (ISRU) will drastically reduce the reliance of supplies from Earth. Lunar resources including mineral resources, water/ice resources, volatiles, and solar energy will contribute to the establishment of a lunar base for long-term life support and scientific exploration missions, although we must consider the challenges from high vacuum, low gravity, extreme temperature conditions, etc. This article provides a comprehensive review of the past developing processes of ISRU and the latest progress of several ISRU technologies, including in situ water access, in situ oxygen production, in situ construction and manufacture, in situ energy utilization, and in situ life support and plant cultivation on the Moon. Despite being able to provide some material and energy supplies for lunar base construction and scientific exploration, the ISRU technologies need continuous validation and upgrade to satisfy the higher requirements from further lunar exploration missions. Ultimately, a 3-step development plan for lunar ISRU technologies in the next decade is proposed, which consists of providing technological solutions, conducting technical verification on payloads, and carrying out in situ experiments, with the ultimate aim of establishing a permanent lunar station and carrying out long-term lunar surface scientific activities. The overview of ISRU techniques and our suggestions will provide potential guidance for China’s future lunar exploration missions.
Pengaruh Environmental Policy, Environmental Pollution, Environmental Energy, Dan Environmental Financial Terhadap Kualitas Laba
Authors: Surbakti Lidya Primta 
Published: May 2023

This research is a quantitative research and the aim of this research is to empirically examine the influence of environmental policy, environmental pollution, environmental energy, and environmental finance on earnings quality and profitability, leverage, and company size as control variables. Earnings quality is measured using real earnings management as a measurement.This study uses secondary data, namely non-financial companies listed on the Indonesia Stock Exchange for the period during 2019-2021 . The total sample in this study was 198 samples using the panel data regression analysis method and using data analysis with STATA. The research results show that environmental policy, environmental pollution, environmental energy, and environmental financial no significant effect on earnings quality. But there is a significant and positive effect on the control variables including: profitability, company size and debt ratio on earnings quality. The limitation of this study is that the population is non-financial companies and only measures the disclosure of sustainability reports only from pollution, energy, finance and policy. The practical implication of this research is that the more sustainability report disclosures made by companies, including: policies, pollution, energy and finance, no significant effect on the quality of earnings in companies listed on the IDX, and the increasing profitability, debt ratios and company size, the higher earnings quality, so that investors can consider profitability, company size and debt ratio in investment decisions because one of the considerations of investors is profit. The original value of this research is that this research measures more specific environmental reporting disclosures including: environmental policy, environmental pollution, environmental energy, and environmental financial and earnings quality measurement in this study uses real earnings management with the Cohen and Zarowin (2010) model which is still rarely used.
EDUKASI SISTEMATIKA PENYUSUNAN LAPORAN KEUANGAN DALAM ORGANISASI NIRLABA
Authors: Wibowo Doni Teguh 
Published: May 2023

Sumbersuko merupakan desa terluas diwilayah kecamatan wagir kabupaten Malang Jawa Timur, desa ini memiliki potensi alam yang sangat luar biasa serta didukung dengan kondisi budaya masyarakat yang sangat beragam. Dengan karakteristik sosial, budaya, dan jenjang pendidikan yang ada dimasyarakat sangat mempengaruhi kagiatan masyarakat dalam berinteraksi dalam suatu wadah organisasi. Kegiatan pengabadian ini diharapakan pengurus dan pengelola organisasi memiliki pengetahuan serta ketrampilan yang memadai terkait pengelolaan organisasi, dengan harapan besar meningkatnya pemahaman terkait sistem pengelolaan sumber daya moneter yang transparan dan akuntabel. Mitra dalam kegiatan pengabdian ini adalah organisasi nirlaba Muslimat NU desa sumbersuko wagir yang bergerak dibidang sosial keagamaan. Peserta kegiatan ini adalah pengurus, pengelola dan anggota, metode pendekatan yang digunakan dalam pelaksanaan program kegiatan pengabdian ini adalah pendekatan edukatif dan parsitipatif. Permasalahan pada mitra timbul akibat adanya keterbatasan pengetahuan dan keterampilan pengelolaan pada organisasi Muslimat NU, permasalahan mitra selama ini adalah membuat laporan keuangan sebatas pada kemampuan dan pemahaman yang dimiliki, dengan sistem pencatatan dan pembukuan yang dilakukan secara manual dan konvensial. Sudah sepatutnya PSAK 45 dijadikan dasar rujukan dalam menyusun pelaporan keuangan, yang menyediakan informasi yang relevan untuk memenuhi kepentingan para penyumbang, anggota entitas nirlaba, kreditor, dan pihak lain yang menyediakan sumber daya bagi entitas nirlaba, yang berlaku secara umum.
ANALISIS USAHA KERUPUK BALOK DITINJAU DARI NILAI TAMBAH DAN PEMASARAN PADA INDUSTRI RUMAH TANGGA RAOS ECHO DI KABUPATEN SELUMA
Authors: Tina Sari Impian , Fariadi Herri , Andriani Evi 
Published: May 2023

Industri kecil disuatu daerah secara ekonomi mampu berkembang dan meningkatkan nilai produksi yang sudah menjadi permintaan konsumen secara kontiniu. Tujuan penelitian ini untuk mengetahui besarnya nilai tambah dan pemasaran kerupuk balok pada industri Raos Echo. Penentuan sampel lokasi penelitian dilakukan secara sengaja (purposive) yakni Usaha kerupuk sagu “Raos Echo”. Responden dalam penelitian ini adalah pimpinan perusahaan dan lembaga-lembaga pemasaran. Data yang digunakan bersumber dari data primer dan data sekunder. Analisis data yang digunakan adalah analisis nilai tambah, saluran pemasaran dan margin pemasaran, hasil penelitian menunjukan bahwa Nilai tambah yang dihasilkan dari Agroindustri Kerupuk Raos Echo yaitu sebesar Rp.11.994,11/kg dengan persentase sebesar 0,32% dan keuntungan sebesar Rp. 10.456,11/kg dengan tingkat keuntungan sebesar 0,87%. Nilai tambah yang diperoleh dari pengolahan sagu menjadi kerupuk di usaha kerupuk Raos Echo tergolong rendah. Pada saluran pemasaran kerupuk Raos Echo di daerah penelitian memiliki 3 saluran pemasaran yaitu saluran I dari produsen ke pedagang pengecer Kota Bengkulu ke konsumen, saluran II dari produsen ke pedagang pengecer Bengkulu Tengah ke konsumen, saluran III konsumen ke produsen. Margin pemasaran saluran pemasaran tingkat ke I sebesar Rp.5000. Margin pemasaran saluran pemasaran tingkat ke II sebesar Rp.6000.
Kata Kunci : Nilai Tambah, Pemasaran Kerupuk Balok, Raos Echo
MOTIF DAN POLA INTERAKSI ANTARA PETANI KELAPA SAWIT DAN LEMBAGA PEMASARAN DI DESA DUSUN BARU I KECAMATAN PONDOK KUBANG KABUPATEN BENGKULU TENGAH
Authors: Sugiman  , Fariadi Heri , Andriani Evi 
Published: May 2023

Di Dusun Baru Kecamatan Pondok Kubang, keberadaan kelembagaan pemasaran pertanian telah berkontribusi dalam meningkatkan produktivitas serta kesejahteraan petani kelapa sawit, dengan memberikan harga TBS yang stabil dalam setiap jual-beli yang terjadi dan memberikan pinjaman kepada petani ketika dibutuhkan. Selain itu memberikan perhatian-perhatian kecil seperti bantuan dalam bentuk uang maupun jasa. Tujuan dari penelitian ini adalah mengetahui motif-motif yang mendorong terjadinya interaksi sosial antara petani kelapa sawit dan lembaga pemasaran, mengetahui pola interaksi sosial yang terjadi antara petani kelapa sawit dan lembaga pemasaran di Desa Dusun Baru I Kecamatan Pondok Kubang Kabupaten Bengkulu Tengah. Metode penelitian yang digunakan adalah metode kualitatif dengan menggunakan analisis deskriptif. Hasil penelitian menunjukkan bahwa motif interaksi sosial antara petani kelapa sawit dan lembaga pemasaran dibagi menjadi 2 (dua) yaitu motif ekonomi dan motif sosial. Untuk motif ekonomi atribut tertinggi adalah motif karena ‘pemasaran hasil pertanian ” yaitu sebesar 100%, kemudian dilanjutkan dengan motif “peminjaman modal usaha” yaitu sebesar 80% dan untuk motifi karena “adanya kebutuhan dasar” yaitu sebesar 45,71%. Sedangakan untuk motif sosial alasan karena “mencari pengalaman” sebesar 54,26%, diikuti dengan motif karena adanya “kebutuhan hubungan dengan orang banyak” yaitu sebesar 28,57%. Motif “menambah pergaulan: sebesar 17,14%. Pola interaksi sosial yang terjadi antara petani kelapasawit dengan lembaga pemasaran di Desa Dusun Baru I Kecamatan Pondok Kubang yaitu pola pertukaran sosial, pola kebisaaan, pola ketergantungan, pola hubungan jual beli antara petani kelapa sawit dan tauke, pola hubungan hutang piutang antara petani kelapa sawit dan lembaga pemasaran dan pola hubungan sosial antara petani kelapa sawit dan lembaga pemasaran.
Kata Kunci: Motif interaksi sosial, pola interaksi sosial, petani kelapa sawit, lembaga pemasaran
PENGARUH PROFESIONALITAS GURU MATEMATIKA DALAM MENINGKATKAN KOMPETENSI SISWA ERA REVOLUSI INDUSTRI 5.0 DI INDONESIA: SEBUAH META-ANALISIS
Authors: Ichsan  , Aulia Sofianora  , Desy  , Yayat Suharyat  , Tomi Apra Santosa  , Agus Supriyadi  
Published: May 2023

Penelitian ini bertujuan untuk mengetahui pengaruh profesional guru matematika dalam meningkatkan kompetensi siswa di Indonesia. Penelitian ini merupakan jenis penelitian meta-analisis. Sumber data dalam penelitian berasal dari analisis 18 jurnal nasional maupun internasional yang terbit dari tahun 2013-2023. Sumber data dalam penelitian diambil melalui database google scholar, Eric, Wiley dan Taylor of Francis.Teknik pengumpulan data dalam penelitian adalah observasi langsung melalui database jurnal. Kata kunci yang digunakan dalam penelusuran sumber data yaitu guru profesional, kompetensi siswa dan pembelajaran matematika. Teknik Analisis data adalah teknik analisis data kuantitatif dengan bantuan aplikasi OpenMEE. Hasil penelitian dapat disimpulkan bahwa perofesional guru matematika mempunyai pengaruh yang signifikan terhadap kompetensi siswa di Indonesia. Nilai effect size sebesar 1.19 kriteria sangat tinggi, Standar deviasi sebesar 0.36 dan N-gain sebesar 0.57. Jadi, untuk memperbaiki kualitas guru matematika harus perlu dilatih profesionalitas yang tinggi dalam melaksanakan kegiatan belajar mengajar.
Kata Kunci: Era Society 5.0, Pembalajaran, Profesionalitas Guru, Kompetensi Siswa
ANALISIS FAKTOR PENYEBAB KESULITAN BELAJAR MATEMATIKA PADA SISWA SMAN 10 REJANG LEBONG MASA PANDEMI COVID-19
Authors: Sarwoedi  
Published: May 2023

Penelitian ini memiliki tujuan untuk melihat faktor apa yang menjadi penyebab siswa kesulitan belajar matematika pada SMAN 10 Rejang Lebong selama pandemi covid-19 serta melihat apa saja upaya dari guru dalam mengatasi kesulitan tersebut. Penelitian ini merupakan penelitian kualitatif dan yang menjadi subyeknya guru kelas XII IPA 1 dan 34 siswa kelas XII IPA 1 SMAN 10 Rejang Lebong. Data dalam penelitian ini diperoleh melalui wawancara, angket. Data dinalisis dengan metode deskriptif kualitatif untuk menggambarkan jenis dan faktor yang menjadi penyebab kesulitan belajar matematika yang dialami oleh siswa dan upaya yang dapat dilakukan guru dalam mengatasi kesulitan belajar matematika. Dari hasil dan pembahasan, dapat disimpulkan bahwa faktor dari dalam diri siswa dan faktor dari luar diri siswa yang menjadi penyebab kesulitan belajar matematika siswa kelas XII IPA 1 SMAN 10 Rejang Lebong pada masa pandemi covid-19.
Kata Kunci: Kesulitan Belajar, Matematika, Covid-19
Analisis Strategi Pemasaran Pembiayaan Murabahah Pada Bank Syariah Indonesia (Studi Kasus Bank Syariah Mandiri KC Simpang Patal Palembang)
Authors: Riyaldi Rama , Aravik Havis , Choirunnisak Choirunnisak 
Published: May 2023

Starategi pemasaran pembiayaan murabahah dan mekanisme pembiayaan murabahah pada Bank Syariah Indonesia Kantor Cabang Simpang Patal Palembang. Tujuan penelitian ini untuk mengetahui bagaimana strategi pemasaran pembiayaan murabahah pada Bank Syariah Indonesia Kantor Cabang Simpang Patal Palembang. . metode penelitian menggunakan penelitian lapangan (field research) dengan pendekatan kualitatif, teknik pengumpulan data observasi, wawancara dan dokumentasi. Hasil dari penelitian ini yaitu strategi pemasaran yang diterapkan oleh Bank Syariah Indonesia Kantor Cabang Simpang Patal Palembang dalam memasarkan produk pembiayaan murabahah dengan menggunakan brosur, spanduk dan media sosial
Peran Mediasi Motivasi pada Pengaruh Servant Leadership Terhadap Kinerja
Authors: Rahayu Muji , Nur Hayati  , Evan Jaelani  , Ivan Aries Setiawan  
Published: May 2023

Latar belakang penelitian ini adalah kinerja dosen Universitas Swasta di Jawa Barat yang berdasarkan data awal belum mencapai target yang telah ditetapkan. Oleh karena itu penelitian ini dilakukan dengan, tujuan untuk mengetahui efek mediasi motivasi yang menjembatani pengaruh positif servant leadership terhadap kinerja. Populasi adalah dosen Universitas Swasta di Jawa Barat, responden sebanyak 102, yang ditentukan dengan menggunakan teknik random sampling. Data yang digunakankan adalah data primer yang diperoleh melalui penyebaran kuesioner.Metode analisis yang digunakan adalah deskriptif dan verifikatif. Berdasarkan hasil uji hipotesis dapat terkonfirmasi bahwa bahwa servant leadership dan motivasi memiliki pengaruh positif terhadap kinerja. sebagai mediasi. motivasi memediasi pengaruh servant Leadership terhadap kinerja. Hal ini berarti bahwa ketika motivasi dosen meningkat maka kinerja meningkat. Hasil ini dapat dimaknai bahwa motivasi memiliki pengaruh tidak langsung terhadap tingkat kinerja dosen., yaitu kinerja yang dihasilkan akan lebih tinggi apabila motivasi yang dimiliki oleh dosen tinggi.