bluuebunny/crossref_metadata_2025_split huggingface dataset:
Psychological impact of COVID‐19 pandemic and lockdown among the population involved in tourism sector in Lakeside of Pokhara: A qualitative study
Authors: Paudel Rajesh , Khadka Seema , Rai Jeetendra , Singh Rakesh 
Published: July 2023

AbstractBackground and AimsThe COVID‐19 pandemic has exerted a substantial influence on every sector of people's lives worldwide, including Nepal. The tourism industry is not exceptional. Lakeside Pokhara is one of the country's major tourist hubs and relies both on national and international visitors. The people residing in this area who depend on tourism‐related businesses to regulate their daily living faced numerous stressors and psychological impacts due to the pandemic. This study aimed to explore the COVID‐19 pandemic‐related stressors and their psychological impact among people dependent on the tourism business in the Lakeside of Pokhara, located in the Gandaki Province of Nepal.MethodVia the qualitative approach, semi‐structured in‐depth interviews were conducted to collect the data from 20 individuals related to tourism business stakeholders in Lakeside of Pokhara. Thematic analysis was performed to analyze the data.ResultsThe study found business‐related stressors among the people dependent on the tourism businesses, and these stressors were found to increase the experiences of psychological issues, including suicidal ideation. The pandemic has not only affected their economy but has also had an impact on their personal, familial, and social life. However, to combat the problems most of the study participants were found to be utilizing positive coping mechanisms, whereas some respondents were observed to consume more alcohol as a negative coping strategy.ConclusionsPeople indulging in the tourism sectors were at greater risk of vulnerability in the future pandemic. Tourism business stakeholders struggled to combat the numerous stressors and psychological impacts carried out by the COVID‐19 pandemic and lockdown. Therefore, there is a growing need for government bodies to implement favorable business‐related policies, and Mental Health and Psychosocial Support (MHPSS) related programs to these stakeholders.
Panitumumab plus trifluridine/tipiracil as anti‐EGFR rechallenge therapy in patients with refractory RAS wild‐type metastatic colorectal cancer: Overall survival and subgroup analysis of the randomized phase II VELO trial
Authors: Napolitano Stefania , Ciardiello Davide , De Falco Vincenzo , Martini Giulia , Martinelli Erika , Della Corte Carminia Maria , Esposito Lucia , Famiglietti Vincenzo , Di Liello Alessandra , Avallone Antonio , Cardone Claudia , De Stefano Alfonso , Montesarchio Vincenzo , Zampino Maria Giulia , Fazio Nicola , Di Maio Massimo , Del Tufo Sara , De Vita Ferdinando , Altucci Lucia , Marrone Francesca , Ciardiello Fortunato , Troiani Teresa 
Published: July 2023

AbstractThe randomized phase II VELO trial showed that the addition of panitumumab to trifluridine/tipiracil significantly improves progression‐free survival (PFS) as compared to trifluridine/tipiracil in third‐line therapy in patients with refractory RAS wild‐type (WT) metastatic colorectal cancer (mCRC). With longer follow‐up, final overall survival results and posttreatment subgroup analysis are presented. Sixty‐two patients with refractory RAS WT mCRC were randomly assigned to receive, as third‐line therapy, trifluridine/tipiracil alone (arm A) or in combination with panitumumab (arm B). Primary endpoint was PFS; secondary endpoints included overall survival (OS) and overall response rate (ORR). Median OS was 13.1 months (95% CI 9.5‐16.7) in arm A compared to 11.6 months (95% CI 6.3‐17.0) in arm B (HR: 0.96, 95% CI 0.54‐1.71, P = .9). To evaluate the impact of subsequent lines of treatment, subgroup analysis was performed for the 24/30 patients in arm A, that received fourth‐line therapy after disease progression. Median PFS was 4.1 months (95% CI 1.44‐6.83) for 17 patients treated with anti‐EGFR rechallenge as compared to 3.0 months (95% CI 1.61‐4.31) for seven patients that received other therapies (HR: 0.29, 95% CI 0.10‐0.85, P = .024). Median OS from the start of fourth‐line treatment was 13.6 months (95% CI 7.2‐20), and 5.1 months (95% CI 1.8‐8.3) for patients treated with anti‐EGFR rechallenge vs other therapies, respectively (HR: 0.30, 95% CI 0.11‐0.81, P = .019). Final results of the VELO trial support the role of anti‐EGFR rechallenge in the continuum of care of patients with RAS/BRAF WT mCRC.
Grading of steatosis, fibrosis, lobular inflammation, and ballooning from liver pathology images using pre‐trained convolutional neural networks
Authors: Zamanian Hamed , Shalbaf Ahmad 
Published: July 2023

AbstractThis study aims to automatically detect the degree of pathological indices as a reference method for detecting the severity and extent of various liver diseases from pathological images of liver tissue with the help of deep learning algorithms. Grading is done using a collection of pre‐trained convolutional neural networks, including DenseNet121, ResNet50, inceptionv3, MobileNet, EfficientNet‐b1, EfficientNet‐b4, Xception, NASNetMobile, and Vgg16. These algorithms are performed by fine‐tuning the trainable layers of the networks. The results showed that compared to other methods, the EfficientNet‐b1 network provides a better response to grade the stage of liver disease among all indicators from pathological images, due to its structural features. This classification accuracy was 97.26% for fibrosis, 94.1% for steatosis, 90.2% for lobular inflammation, and 98.0% for ballooning. Consequently, this fully automated framework can be very useful in clinical methods and be considered as an assistant or an alternative to the diagnosis of experienced pathologists.
Organoid and organoid extracellular vesicles for osteoporotic fractures therapy: Current status and future perspectives
Authors: Liu Han , Su Jiacan 
Published: July 2023

AbstractOsteoporosis is a systemic and degenerative disease characterized by low bone mass and fragile microarchitecture, which predispose patients to fragility fractures, also known as osteoporotic fractures (OPF). OPF have become a major social problem that threaten the health of the elderly. Over the past decade, organoids and organoids extracellular vesicles (OEVs) play a significant role in OPF repair. Organoids have been widely used for fractures treatment. Moreover, EVs are promising nanocarriers due to their cell‐free system, stable drug loading capacity, nanometer size, and good biocompatibility. Importanly, compared with traditional EVs, OEVs have more quantity, better physiological effects, and better therapeutic effects. Therefore, the development of organoid and OEVs in the treatment of OPF is of great significance. Here, we summarize the current status and future perspectives of organoids and OEVs, which will provide innovative solutions to OPF repair.
CircDUS2L (circ_0039908) promotes lung adenocarcinoma progression by upregulating PGAM1 by acting as a miR‐590‐5p molecular sponge
Authors: Tian Qing , Guo Ying , Liu Jinfeng , Pang Chao , Wang Qiang , Xie Qi , Li Jianke 
Published: July 2023

AbstractLung adenocarcinoma (LUAD) is usually found at the metastatic stage. Circular RNA dihydrouridine synthase 2‐like (DUS2L) (circDUS2L) has been discovered to be upregulated in LUAD. Nevertheless, the function of circDUS2L in LUAD has not been verified. Levels of circDUS2L, microRNA‐590‐5p (miR‐590‐5p), and phosphoglycerate mutase 1 (PGAM1) mRNA were analyzed using quantitative real‐time polymerase chain reaction (RT‐qPCR). Cell proliferation, apoptosis, metastasis, and invasion were assessed by 3‐(4,5‐Dimethylthiazol‐2‐yl)‐2,5‐Diphenyltetrazolium Bromide (MTT), colony formation, 5‐ethynyl‐2′‐deoxyuridine (Edu), flow cytometry, and transwell assays. Protein levels were detected by western blotting. Cell glycolysis was analyzed by measuring cell glucose consumption, lactate production, and extracellular acidification rate (ECAR). The regulatory mechanism of circDUS2L in LUAD cells was analyzed by bioinformatics analysis, dual‐luciferase reporter, RNA pull‐down, and RNA immunoprecipitation (RIP) assays. Xenograft assay was conducted to confirm the function of circDUS2L in vivo. CircDUS2L was highly expressed in LUAD tissues and cells. CircDUS2L silencing constrained xenograft tumor growth in vivo. CircDUS2L knockdown induced apoptosis, repressed viability, colony formation, proliferation, metastasis, invasion, and glycolysis of LUAD cells in vitro by releasing miR‐590‐5p via functioning as a miR‐590‐5p sponge. MiR‐590‐5p was lowly expressed in LUAD tissues and cells, and miR‐590‐5p mimic curbed malignant behaviors and glycolysis of LUAD cells by targeting PGAM1. PGAM1 was overexpressed in LUAD tissues and cells, and circDUS2L sponged miR‐590‐5p to regulate PGAM1 expression. CircDUS2L elevated PGAM1 expression through functioning as a miR‐590‐5p sponge, thus driving malignant behaviors and glycolysis of LUAD cells.
Posttraumatic sleep disturbances in veterans: A pilot randomized controlled trial of cognitive behavioral therapy for insomnia and imagery rehearsal therapy
Authors: Prguda Emina , Evans Justine , McLeay Sarah , Romaniuk Madeline , Phelps Andrea J. , Lewis Kerri , Brown Kelly , Fisher Gina , Lowrie Fraser , Saunders‐Dow Elise , Dwyer Miriam 
Published: July 2023

AbstractObjectivesPosttraumatic stress disorder (PTSD) is associated with sleep disturbances including insomnia and nightmares. This study compared cognitive behavioral therapy for insomnia (CBT‐I) with CBT‐I combined with imagery rehearsal therapy (IRT) for nightmares to evaluate if the combined treatment led to greater reductions in trauma‐related sleep disturbances in Australian veterans.MethodsVeterans with diagnosed PTSD, high insomnia symptom severity, and nightmares (N = 31) were randomized to eight group CBT‐I sessions or eight group CBT‐I + IRT sessions. Self‐reported sleep, nightmare, and psychological measures (primary outcome: Pittsburgh Sleep Quality Index), and objective actigraphy data were collected; the effect of obstructive sleep apnea (OSA) risk on treatment outcomes was also examined.ResultsNo treatment condition effects were detected for the combined treatment compared to CBT‐I alone, and no moderating effect of OSA risk was detected. On average, participants from both groups improved on various self‐report measures over time (baseline to 3 months posttreatment). Despite the improvements, mean scores for sleep‐specific measures remained indicative of poor sleep quality. There were also no significant differences between the groups on the actigraphy indices.ConclusionsThe findings indicate that there is potential to optimize both treatments for veterans with trauma‐related sleep disturbances.
How communication mediums shape the message
Authors: Oba Demi , Berger Jonah 
Published: July 2023

AbstractCommunication is an integral part of everyday life. Consumers chat with friends, search for information, and complain to customer service. Salespeople pitch products, employees answer questions, and market researchers ask them. But communication does not occur in a vacuum. Modalities (e.g., speaking or writing), channels (e.g., text, phone call, or email), and devices (e.g., smartphone or computer) are the mediums through which communicators communicate. While these mediums often seem incidental, might they impact what gets communicated? And if so, how? This paper offers a comprehensive framework for understanding how mediums shape the message. Specifically, we argue that modality, devices, and channels all shape communication through the same two key drivers: deliberation and audience salience. As a result, the mediums communicators use to communicate impact everything from the thoughtfulness and concreteness of communicated content to the degree to which it is self‐enhancing or honest. This work sheds light on the psychology of content production, provides insight into the drivers and consequences of communication, and highlights how emerging technologies may shape communication in the future.
Intended and unintended impacts of nitrogen‐fixing microorganisms and microbial inhibitors on nitrogen losses in contrasting maize cropping systems
Authors: Souza Emerson F. C. , Rosen Carl J. , Venterea Rodney T. , Tahir Muhammad 
Published: July 2023

AbstractEfforts to mitigate the nitrogen (N) footprint of maize production include using N‐fixing microbes (NFM) and/or microbial inhibitors. We quantified the effects of NFM, the nitrification inhibitor (NI) 2‐(N‐3,4‐dimethyl‐1H‐pyrazol‐1‐yl) succinic acid isomeric mixture, and the urease inhibitor (UI) N‐(n‐butyl) thiophosphoric triamide, each applied by itself or paired with another additive, on nitrous oxide (N2O) emissions, nitrate (NO3−) leaching, and crop performance in contrasting irrigated and rainfed maize systems over two growing seasons. We also used published emission factors to estimate indirect N2O emissions from leached NO3− that can be converted to N2O. Agronomic effects were relatively small; the NI + NFM treatment increased N use efficiency and grain yield and protein content in some cases by 11%–14% relative to a treatment receiving only urea. Most of the additive treatments reduced direct (in‐field) N2O emissions, most consistently for treatments that contained NI which reduced emissions by 24%–77%. However, these beneficial effects were counteracted by increased NO3− leaching, which occurred most consistently with UI or NFM applied as single additives or with NI. In these treatments, NO3− leaching increased during at least one growing season, and at both sites, by factors of 2–7. In three site‐years, increased NO3− leaching with NFM and NI + NFM offset large reductions in direct N2O, such that total direct + indirect N2O emissions were not different from that in the urea only treatment. These unintended effects may have resulted from unfavorable rainfall timing, varying crop N demand, and declining additive effectiveness. Use of these soil additives requires caution and further study.
GRIK1‐AS1 deficiency accelerates endometriosis progression by boosting DNMT1‐dependent SFRP1 promoter methylation in endometrial stromal cells
Authors: Liu Wei , Hu Bin , Wang Xiaoli , Huang Erqing , Chen Xuexing , Chen Lijuan 
Published: July 2023

AbstractBackgroundEndometriosis, a gynecological disease that affects up to 10% of women, is a major cause of pain and infertility. Deregulation of the epigenome is accountable for the onset and progression of endometriosis, although its exact mechanism is unknown. The purpose of the current study is to examine the role of the long non‐coding RNA (lncRNA) GRIK1‐AS1 in the epigenetic regulation of endometrial stromal cell proliferation and the development of endometriosis.MethodsEndometriosis datasets were screened to identify GRIKI‐AS1 as dramatically declining in endometriosis. Gain or loss of function endometrial stromal cell (ESC) models were established. The anti‐proliferation phenotype was investigated using in vitro and in vivo experiments. Epigenetic regulatory network analyses were conducted to suggest the intrinsic molecular mechanism.ResultsWith bioinformatic and clinical data, we observed that GRIK1‐AS1 and SFRP1 were expressed at low levels in endometriosis. Overexpressed GRIK1‐AS1 inhibited ESC proliferation, while SFRP1 knockdown rescued the antiproliferative ability of GRIK1‐AS1. Specifically, methylation‐dependent expression inhibition of SFRP1 was revealed in ESCs. Mechanistically, GRIK1‐AS1 hampers the occupancy of DNMT1 in SRFP1 promoter, leading to hypomethylation of SFRP1 and upregulated SFRP1 expression, thereby potentially suppressing Wnt signaling and its adverse proliferative effect. Therapeutically, lentivirus‐mediated upregulation of GRIK1‐AS1 inhibited endometriosis disease progression in vivo.ConclusionsOur study is a proof‐of‐concept demonstration for GRIKI‐AS1‐associated endometriosis pathogenesis and highlights a potential intervention target.
Identification of the shared gene signatures and molecular pathways in systemic lupus erythematosus and diffuse large B‐cell lymphoma
Authors: Li Haoguang , Zhou Jie , Zhou Lu , Zhang Xiuling , Shang Jingjing , Feng Xueqin , Yu Le , Fan Jie , Ren Jie , Zhang Rongwei , Duan Xinwang 
Published: July 2023

AbstractBackgroundDiffuse large B‐cell lymphoma (DLBCL) incidence is higher in systemic lupus erythematosus (SLE) patients than the general population, but the molecular mechanisms behind this link remain ambiguous. The aim of this study was to investigate shared gene signatures and molecular pathways between SLE and DLBCL.MethodsWe procured expression profiles of SLE and DLBCL from public databases and identified common differentially expressed genes (DEGs). Functional pathway enrichment and protein–protein interaction (PPI) analyses were performed on these shared genes. The molecular complex detection technology (MCODE) and eXtreme Gradient Boosting (XGBoost) machine learning algorithm were used to select core shared genes, followed by Gene Set Enrichment Analysis (GSEA) and immune infiltration analysis.ResultsWe identified 54 DEGs as shared genes, among which CD177, CEACAM1, GPR84 and IFIT3 were identified as core shared genes. These genes showed strong associations with inflammatory and immune response pathways. We found a significant positive correlation between GPR84 and IFIT3 expression levels and the immune microenvironment. Decreased expression levels of GPR84 and IFIT3 were linked to enhanced immune therapy sensitivity, potentially due to lower dysregulation scores during low expression. We also discovered that TP53 mutations might elevate CD177 and GPR84 expression and that reduced expression levels of GPR84 and IFIT3 were linked with better overall survival and progression‐free survival in DLBCL patients.ConclusionsOur study provides valuable insights into the shared molecular mechanisms underpinning the pathogenesis of SLE and DLBCL. These findings could potentially offer new biomarkers and therapeutic targets for SLE and DLBCL.
Regioselective synthesis and computational calculation studies of some new pyrazolyl‐pyridine and bipyridine derivatives
Authors: Farag Ahmad M. , Kheder Nabila A. , Fahim Asmaa M. , Dawood Kamal M. 
Published: July 2023

AbstractStarting from 3‐oxo‐N‐(3‐pyridyl)butanamide, as key intermediate, the title compounds were synthesized in good yields. Reaction of the pyridine‐carboxamide 1 with hydrazonoyl chlorides 3a,b and with triethyl orthoformate afforded the corresponding pyrazole‐carboxamides 6a,b, and the bis‐pyridine 7, respectively. Coupling of the pyridine‐carboxamide 1 with 3‐phenylpyrazole‐5‐diazonium chloride gave the hydrazone 10 which upon heating in pyridine afforded the pyrazolo[5,1‐c]‐1,2,4‐triazine derivative 11. Reaction of the pyridine‐carboxamide 1 with arylmethylenepropanedinitrile derivatives afforded 1,3′‐bipyridines 13a–c. Structure elucidation of the newly synthesized compounds was carried out using IR, NMR spectroscopy, and mass spectrometry. The extensive theoretical and experimental mechanical studies of the pyrazole derivative 6a were in accordance with its elemental analysis, FT‐IR, NMR, and MS spectral data. Moreover, computer analyses indicated that the pyridine derivatives 13a,b predominated over the pyran derivatives 14a,b as the adducts. In addition, the optimization of compounds utilized the HF/6‐31G(d) and DFT/B3LYP/6‐31G(d) basis sets. Furthermore, the physical descriptors of these compounds demonstrated their stability, and NMR correlation investigations were also carried out.
Deep Learning Nomogram for the Identification of Deep Stromal Invasion in Patients With Early‐Stage Cervical Adenocarcinoma and Adenosquamous Carcinoma: A Multicenter Study
Authors: Xiao Mei Ling , Qian Ting , Fu Le , Wei Yan , Ma Feng Hua , Gu Wei Yong , Li Hai Ming , Li Yong Ai , Qian Zhao Xia , Cheng Jie Jun , Zhang Guo Fu , Qiang Jin Wei 
Published: July 2023

BackgroundDeep stromal invasion (DSI) is one of the predominant risk factors that determined the types of radical hysterectomy (RH). Thus, the accurate assessment of DSI in cervical adenocarcinoma (AC)/adenosquamous carcinoma (ASC) can facilitate optimal therapy decision.PurposeTo develop a nomogram to identify DSI in cervical AC/ASC.Study TypeRetrospective.PopulationSix hundred and fifty patients (mean age of 48.2 years) were collected from center 1 (primary cohort, 536), centers 2 and 3 (external validation cohorts 1 and 2, 62 and 52).Field Strength/Sequence5‐T, T2‐weighted imaging (T2WI, SE/FSE), diffusion‐weighted imaging (DWI, EPI), and contrast‐enhanced T1‐weighted imaging (CE‐T1WI, VIBE/LAVA).AssessmentThe DSI was defined as the outer 1/3 stromal invasion on pathology. The region of interest (ROI) contained the tumor and 3 mm peritumoral area. The ROIs of T2WI, DWI, and CE‐T1WI were separately imported into Resnet18 to calculate the DL scores (TDS, DDS, and CDS). The clinical characteristics were retrieved from medical records or MRI data assessment. The clinical model and nomogram were constructed by integrating clinical independent risk factors only and further combining DL scores based on primary cohort and were validated in two external validation cohorts.Statistical TestsStudent's t‐test, Mann–Whitney U test, or Chi‐squared test were used to compare differences in continuous or categorical variables between DSI‐positive and DSI‐negative groups. DeLong test was used to compare AU‐ROC values of DL scores, clinical model, and nomogram.ResultsThe nomogram integrating menopause, disruption of cervical stromal ring (DCSRMR), DDS, and TDS achieved AU‐ROCs of 0.933, 0.807, and 0.817 in evaluating DSI in primary and external validation cohorts. The nomogram had superior diagnostic ability to clinical model and DL scores in primary cohort (all P < 0.0125 [0.05/4]) and CDS (P = 0.009) in external validation cohort 2.Data ConclusionThe nomogram achieved good performance for evaluating DSI in cervical AC/ASC.Level of Evidence3Technical EfficacyStage 2
How Crisis May Generate and Sustain Creative Cycles: The Role of Problem Persistence
Authors: Villanova Ana Luisa , Pina e Cunha Miguel , Carlsen Arne 
Published: July 2023

ABSTRACTWe performed an inductive study to advance theory on how a crisis can inspire individuals to be persistently creative in successive cycles. We draw from rich data of 17 volunteer projects in the Tech4Covid movement, a Portuguese organization of entrepreneurs who gathered online to develop digital solutions to help society during the COVID‐19 pandemic. This empirical context is uniquely suited to study how interactions with intended beneficiaries during crises can encourage creators to initiate and continue creative work. Our results allowed us to extend the knowledge of crisis‐induced creative processes in two ways. First, we noticed that throughout the creative process, creators might switch the primary focus of their work from outside beneficiaries to their own benefit. These changes can serve as a trigger to reinforce creators' motivations to continue their creative work beyond the first set of creative outputs. Second, we propose that the nature of the problem to be solved influences the continuity of creative processes: while momentary problems induced by the crisis may stimulate episodic ideas, their transitory nature may prevent creators from having time to fully develop their ideas further. Thus, it is primarily persistent problems that favor the progress of ideas in successive creative cycles.