bluuebunny/crossref_metadata_2025_split huggingface dataset:
Exposure of preterm neonates receiving total parenteral nutrition to phthalates and its impact on neurodevelopment at the age of 2 months
Authors: Al-Saleh Iman , Elkhatib Rola , Alnuwaysir Hissah , Aldhalaan Hesham , Alismail Eiman , Binmanee Abdulaziz , Hawari Amal , Alhazzani Fahad , Jabr Mohammad Bin , Mohamed Gamal 
Published: April 2023

AbstractThis prospective study assessed the exposure to phthalates of preterm neonates who received total parenteral nutrition (TPN) during their stay in the neonatal intensive care unit (NICU) and the risk of neurodevelopment delays at the age of 2 months. Our study recruited 33 preterm neonates who required TPN upon NICU admission. Urine samples for analyzing phthalate metabolites were obtained at admission and then daily until the last day of receiving TPN. Phthalates in the daily TPN received by the preterm neonates were analyzed. The neurodevelopment of the neonates was assessed using the Ages and Stages Questionnaire Edition 3 (ASQ-3). Diethyl phthalate and butyl benzyl phthalate were found in all TPN samples, while 27% and 83% contained dibutyl phthalate and di-(2-ethylhexyl) phthalate (DEHP), respectively. Yet, the daily dose of each phthalate that our preterm neonates received from TPN was much lower than the recommended tolerable limit. Urinary levels of monobenzyl phthalate and four metabolites of DEHP [i.e., mono-(2-ethylhexyl) phthalate (MEHP), mono-(2-ethyl-5-hydroxyhexyl) phthalate, mono-(2-ethyl-5-oxohexyl) phthalate (MEOHP), and mono-(2-ethyl-5-carboxypentyl) phthalate (MECPP)] and the sum of four DEHP metabolites (∑4DEHP) increased significantly in preterm neonates before discharge. However, these levels were not correlated with their phthalate parent compounds in TPN, suggesting other sources of exposure in the NICU. At 2 months, we found that urinary levels of mono-iso-butyl phthalate (MiBP), MECPP, MEHP, and ∑4DEHP were inversely related to fine motor skills. After adjusting for head circumference, the inverse relationships remained significant, suggesting direct effects from phthalates. Given the extreme vulnerability of our population, it is critical to minimize exposure to phthalates during their NICU stay.
Comparing x-ray phase-contrast imaging using a Talbot array illuminator to propagation-based imaging for non-homogeneous biomedical samples
Authors: Riedel Mirko , Taphorn Kirsten , Gustschin Alex , Busse Madleen , Hammel Joerg U. , Moosmann Julian , Beckmann Felix , Fischer Florian , Thibault Pierre , Herzen Julia 
Published: April 2023

AbstractPhase-contrast computed tomography can visualize soft tissue samples with high contrast. At coherent sources, propagation-based imaging (PBI) techniques are among the most common, as they are easy to implement and produce high-resolution images. Their downside is a low degree of quantitative data due to simplifying assumptions of the sample properties in the reconstruction. These assumptions can be avoided, by using quantitative phase-contrast techniques as an alternative. However, these often compromise spatial resolution and require complicated setups. In order to overcome this limitation, we designed and constructed a new imaging setup using a 2D Talbot array illuminator as a wavefront marker and speckle-based imaging phase-retrieval techniques. We developed a post-processing chain that can compensate for wavefront marker drifts and that improves the overall sensitivity. By comparing two measurements of biomedical samples, we demonstrate that the spatial resolution of our setup is comparable to the one of PBI scans while being able to successfully image a sample that breaks the typical homogeneity assumption used in PBI.
Optimal water supply reservoir operation by leveraging the meta-heuristic Harris Hawks algorithms and opposite based learning technique
Authors: Lai V. , Huang Y. F. , Koo C. H. , Ahmed Ali Najah , Sherif Mohsen , El-Shafie Ahmed 
Published: April 2023

AbstractTo ease water scarcity, dynamic programming, stochastic dynamic programming, and heuristic algorithms have been applied to solve problem matters related to water resources. Development, operation, and management are vital in a reservoir operating policy, especially when the reservoir serves a complex objective. In this study, an attempt via metaheuristic algorithms, namely the Harris Hawks Optimisation (HHO) Algorithm and the Opposite Based Learning of HHO (OBL-HHO) are made to minimise the water deficit as well as mitigate floods at downstream of the Klang Gate Dam (KGD). Due to trade-offs between water supply and flood management, the HHO and OBL-HHO models have configurable thresholds to optimise the KGD reservoir operation. To determine the efficacy of the HHO and OBL-HHO in reservoir optimisation, reliability, vulnerability, and resilience are risk measures evaluated. If inflow categories are omitted, the OBL-HHO meets 71.49% of demand compared to 54.83% for the standalone HHO. The HHO proved superior to OBL-HHO in satisfying demand during medium inflows, achieving 38.60% compared to 20.61%, even though the HHO may have experienced water loss at the end of the storage level. The HHO is still a promising method, as proven by its reliability and resilience indices compared to other published heuristic algorithms: at 62.50% and 1.56, respectively. The Artificial Bee Colony (ABC) outcomes satisfied demand at 61.36%, 59.47% with the Particle Swarm Optimisation (PSO), 55.68% with the real-coded Genetic Algorithm (GA), and 23.5 percent with the binary GA. For resilience, the ABC scored 0.16, PSO scored 0.15, and real coded GA scored 0.14 whilst the binary-GA has the worst failure recovery algorithm with 0.09.
CBD hydroxyquinone photo-isomerises to a highly reactive intermediate
Authors: Thomson Brodie. J. , Hanna Summer , Schwarzenberg Adrian , Kiani Pirouz , Bizzotto Dan , Kennepohl Pierre , Davies Ashley , Roggen Markus , Sammis Glenn M. 
Published: April 2023

AbstractThe legalisation of hemp has led to wide commercial availability of cannabidiol (CBD)-containing products. Here we show that the CBD-hydroxyquinone (HU-331), a readily formed oxidation product and common impurity in CBD isolates, undergoes a previously unknown photo-isomerisation to produce a highly reactive intermediate in solution. Studies supported by calculations indicate that this intermediate rapidly reacts with oxygen to form a multitude of cannabinoid products. The purple colour observed in light-aged CBD-containing solutions is largely due to the anions of these by-products and is not significantly due to the HU-331 anion. Our findings suggest that these uncharacterized cannabinoid derivatives can be present in CBD-containing e-liquids and solutions that have been stored under ambient light conditions, calling for quality control processes that manage HU-331 contamination.
Preliminary evaluation of deep learning for first-line diagnostic prediction of tumor mutational status
Authors: Morel Louis-Oscar , Derangère Valentin , Arnould Laurent , Ladoire Sylvain , Vinçon Nathan 
Published: April 2023

AbstractThe detection of tumour gene mutations by DNA or RNA sequencing is crucial for the prescription of effective targeted therapies. Recent developments showed promising results for tumoral mutational status prediction using new deep learning based methods on histopathological images. However, it is still unknown whether these methods can be useful aside from sequencing methods for efficient population diagnosis. In this retrospective study, we use a standard prediction pipeline based on a convolutional neural network for the detection of cancer driver genomic alterations in The Cancer Genome Atlas (TCGA) breast (BRCA, n = 719), lung (LUAD, n = 541) and colon (COAD, n = 459) cancer datasets. We propose 3 diagnostic strategies using deep learning methods as first-line diagnostic tools. Focusing on cancer driver genes such as KRAS, EGFR or TP53, we show that these methods help reduce DNA sequencing by up to 49.9% with a high sensitivity (95%). In a context of limited resources, these methods increase sensitivity up to 69.8% at a 30% capacity of DNA sequencing tests, up to 85.1% at a 50% capacity, and up to 91.8% at a 70% capacity. These methods can also be used to prioritize patients with a positive predictive value up to 90.6% in the 10% patient most at risk of being mutated. Limitations of this study include the lack of external validation on non-TCGA data, dependence on prevalence of mutations in datasets, and use of a standard DL method on a limited dataset. Future studies using state-of-the-art methods and larger datasets are needed for better evaluation and clinical implementation.
Annexins A2 and A5 are potential early biomarkers of hepatocarcinogenesis
Authors: Herrera-López Ema Elvira , Guerrero-Escalera Dafne , Aguirre-Maldonado Isaac , López-Hernández Arely , Montero Hilda , Gutiérrez‐Nava María Angélica , del Pozo-Yauner Luis , Arellanes-Robledo Jaime , Camacho Javier , Pérez-Carreón Julio Isael 
Published: April 2023

AbstractHepatocellular carcinoma (HCC) is a highly lethal liver cancer with late diagnosis; therefore, the identification of new early biomarkers could help reduce mortality. We determine the tissue and plasma status of five annexins during hepatocarcinogenesis by diethylnitrosamine-induced cirrhosis-HCC. We found thatAnxa5was the earliest upregulated gene at week 12 after HCC initiation, whileAnxa1andAnxa2were upregulated in advanced HCC stages (weeks 18 and 22). Furthermore, the protein level of Annexin A1, A2, A5 and A10 was increased from the early stages. Immunofluorescence and subcellular fractionation revealed Annexin A1, A2, and A5 in the cytoplasm and nuclei of tumor cells. Notably, increased plasma levels of Annexin A5 significantly (r2 = 0.8203) correlated with Annexin A5 levels in liver tissue from week 12 and gradually increased until week 22. Using the TCGA database, we found that the expression ofANXA2(HR = 1.7,p = 0.0046) andANXA5(HR = 1.8,p = 0.00077) was associated with poor survival in HCC patients. In conclusion, we have identified Annexin A1 and A5 as potentially useful early biomarkers for poor prognosis in HCC patients.
A blue-shifted anion channelrhodopsin from the Colpodellida alga Vitrella brassicaformis
Authors: Kojima Keiichi , Kawanishi Shiho , Nishimura Yosuke , Hasegawa Masumi , Nakao Shin , Nagata Yuya , Yoshizawa Susumu , Sudo Yuki 
Published: April 2023

AbstractMicrobial rhodopsins, a family of photoreceptive membrane proteins containing the chromophore retinal, show a variety of light-dependent molecular functions. Channelrhodopsins work as light-gated ion channels and are widely utilized for optogenetics, which is a method for controlling neural activities by light. Since two cation channelrhodopsins were identified from the chlorophyte alga Chlamydomonas reinhardtii, recent advances in genomic research have revealed a wide variety of channelrhodopsins including anion channelrhodopsins (ACRs), describing their highly diversified molecular properties (e.g., spectral sensitivity, kinetics and ion selectivity). Here, we report two channelrhodopsin-like rhodopsins from the Colpodellida alga Vitrella brassicaformis, which are phylogenetically distinct from the known channelrhodopsins. Spectroscopic and electrophysiological analyses indicated that these rhodopsins are green- and blue-sensitive pigments (λmax =  ~ 550 and ~ 440 nm) that exhibit light-dependent ion channeling activities. Detailed electrophysiological analysis revealed that one of them works as a monovalent anion (Cl−, Br− and NO3−) channel and we named it V. brassicaformis anion channelrhodopsin-2, VbACR2. Importantly, the absorption maximum of VbACR2 (~ 440 nm) is blue-shifted among the known ACRs. Thus, we identified the new blue-shifted ACR, which leads to the expansion of the molecular diversity of ACRs.
Effects of extremely low frequency electromagnetic fields on the tumor cell inhibition and the possible mechanism
Authors: Sun Jie , Tong Yingying , Jia Yu , Jia Xu , Wang Hua , Chen Yang , Wu Jiamin , Jin Weiyang , Ma Zheng , Cao Kai , Li Xiangdong , Chen Zhonglin , Yang Guanghua 
Published: April 2023

AbstractLow-frequency magnetic fields exert a significant inhibitory effect on tumor growth and have been developed as a therapeutic modality. However, the effect of a low-frequency magnetic field on the interaction between cells is still poorly understood. This study aimed to preliminarily evaluate the direct effect of magnetic field ditectely on cultured cells and indirect effect mediated by cell-environment (conditioned medium). 293 T cells, Hepg2 cells, A549 cells have been cultured at 37 ± 0.18 °C in presence of an extremely low-frequency magnetic field of 20 Hz, 5-mT. The adherent tumor cells were more sensitive to magnetic field inhibition in the original environment (conditioned medium) with adherence inhibition rate for Hepg2 and A549 estimated at 18% and 30% respectively. The inhibition effect was suppressed when the suspended cells separated or clump density at a low density. The nontumor cell lines showed no inhibitory effect on exposure to a low-frequency magnetic field. The intracellular ion fluorescence (IIF) showed that the magnetic field significantly altered the membrane potential, indicating hyperpolarization of the adherent cells (ΔIIF 293 T cells: − 25%, ΔIIF Hepg2 cells: − 20% and ΔIIF A549 cells: − 13%) and depolarization of the suspended cells (ΔIIF Raji cells: + 9%). In addition, the conditioned media collected after magnetic field exposure acted on unexposed tumor cells and caused inhibition. Our findings might provide a basis for the mechanism of magnetic field interaction between cells and cell environment in the future.
Muscle synergies in Parkinson’s disease before and after the deep brain stimulation of the bilateral subthalamic nucleus
Authors: Ghislieri Marco , Lanotte Michele , Knaflitz Marco , Rizzi Laura , Agostini Valentina 
Published: April 2023

AbstractThe aim of this study is to quantitatively assess motor control changes in Parkinson’s disease (PD) patients after bilateral deep brain stimulation of the subthalamic nucleus (STN-DBS), based on a novel muscle synergy evaluation approach. A group of 20 PD patients evaluated at baseline (before surgery, T0), at 3 months (T1), and at 12 months (T2) after STN-DBS surgery, as well as a group of 20 age-matched healthy control subjects, underwent an instrumented gait analysis, including surface electromyography recordings from 12 muscles. A smaller number of muscle synergies was found in PD patients (4 muscle synergies, at each time point) compared to control subjects (5 muscle synergies). The neuromuscular robustness of PD patients—that at T0 was smaller with respect to controls (PD T0: 69.3 ± 2.2% vs. Controls: 77.6 ± 1.8%, p = 0.004)—increased at T1 (75.8 ± 1.8%), becoming not different from that of controls at T2 (77.5 ± 1.9%). The muscle synergies analysis may offer clinicians new knowledge on the neuromuscular structure underlying PD motor types of behavior and how they can improve after electroceutical STN-DBS therapy.
Laser isotope separation of 223Ra
Authors: Suryanarayana M. V. 
Published: April 2023

AbstractA three-step photoionization has been theoretically studied for the laser isotope separation of 223Ra through the following photoionization scheme. $$\begin{aligned}7s^{2} {}^{1}S_{0} \left( {0.0 \;{\text{cm}}^{ - 1} } \right)\xrightarrow{{714.3185\;{\text{nm}}}} 7s7p\;{}^{3}P_{1}^{o} \left( {13999.3569\;{\text{cm}}^{ - 1} } \right) \xrightarrow{{784.0270\;{\text{nm}}}}\\7s8s\;{}^{3}S_{1} \left( {26754.02 {\text{cm}}^{ - 1} } \right) \xrightarrow{{558/581\;{\text{nm}}}} {\text{Autoionization State}} \to {\text{Ra}}^{ + }\end{aligned}$$

7

s
2

1

S
0

0.0

cm

-
1

→

714.3185

nm

7
s
7
p

3

P

1

o

13999.3569

cm

-
1

→

784.0270

nm

7
s
8
s

3

S
1

26754.02

cm

-
1

→

558
/
581

nm

Autoionization State

→

Ra

+

The effect of bandwidth, peak power density of the excitation and ionization lasers, Doppler broadening of the atomic ensemble, number density of the atoms, and charge exchange collisions on the laser isotope separation process has been studied. The optimum system parameters for the separation of 223Ra through this photoionization scheme have been derived. The effect of unknown parameters on the degree of enrichment has also been discussed. It has been theoretically shown that it is possible to produce 223Ra isotope with 98.5% radio-isotopic purity at a rate of 0.74 μg/h corresponding to the production rate of 435 patient doses per hour. This is the first ever study on the laser isotope separation of Radium isotopes.
The effect of RCA pavements on the liquefaction-induced settlement
Authors: Akbas Merve , Subasi Ozan , Iyisan Recep 
Published: April 2023

AbstractThe use of recycled concrete aggregates (RCA) not only reduces the demand for natural aggregates (NA) but also might improve the behavior of soil under earthquake loading. In this study, the behavior of the pavement constructed using 100% RCA and NA on a sandy soil layer with high liquefaction potential under dynamic loads was investigated by numerical analysis and compared with free field conditions. For this purpose, first, the classical geotechnical properties of 100% RCA and NA materials were obtained, and then the stiffness properties were determined by resilient modulus and permanent deformation tests. In the second stage, three different models were created with pavement with NA and RCA and without pavement on high liquefaction potential sand, and dynamic analyses were carried out by PM4Sand. Numerical analyses show that constructing a subbase and base layers significantly reduces liquefaction-induced settlement on the surface of flexible pavements built on liquefied soils. Moreover, when the case of using RCA instead of NA in the subbase and base layers built on the liquefied soil is examined, the liquefaction-induced settlement values on the surface are obtained either lower or very close. Consequently, this study proposes a new alternative to the use of RCA waste materials instead of NA. It is expected the use of these waste materials will reduce the need for storage space and also reduce the negative environmental effects associated with storage.
Large-scale clustering of buried X-ray AGN: Trends in AGN obscuration and redshift evolution
Authors: Viitanen A. , Allevato V. , Finoguenov A. , Shankar F. , Gilli R. , Lanzuisi G. , Vito F. 
Published: April 2023

Aims. In order to test active galactic nucleus (AGN) unification and evolutionary models, we measured the AGN clustering properties as a function of AGN obscuration defined in terms of hydrogen column density, NH. In addition to measuring the clustering of unobscured (NH < 1022 cm−2) and moderately obscured (1022 ≤ NH < 1023.5) AGNs, we also targeted highly obscured sources (NH ≥ 1023.5) up to redshifts of z = 3.
Methods. We have compiled one of the largest samples of X-ray-selected AGNs from a total of eight deep XMM/Chandra and multiwavelength surveys. We measured the clustering as a function of both AGN obscuration and redshift using the projected two-point correlation function, wp(rp). We modeled the large-scale clustering signal, measured the AGN bias, b(z, NH), and interpreted it in terms of the typical AGN host dark matter halo, Mhalo(z, NH).
Results. We find no significant dependence of AGN clustering on obscuration, suggesting similar typical masses of the hosting halos as a function of NH. This result matches expectations of AGN unification models, in which AGN obscuration depends mainly on the viewing angle of the obscuring torus. We measured, for the first time, the clustering of highly obscured AGNs and find that these objects reside in halos with typical mass log Mhalo = 12.98−0.22+0.17[h−1 M⊙] (12.28−0.19+0.13) at low z ∼ 0.7 (high z ∼ 1.8) redshifts. We find that irrespective of obscuration, an increase in AGN bias with redshift is slower than the expectation for a constant halo mass and instead follows the growth rate of halos, known as the passive evolution track. This implies that for those AGNs the clustering is mainly driven by the mass growth rate of the hosting halos and galaxies across cosmic time.
Transition disc nature of post-AGB binary systems confirmed by mid-infrared interferometry
Authors: Corporaal A. , Kluska J. , Van Winckel H. , Andrych K. , Cuello N. , Kamath D. , Mérand A. 
Published: April 2023

Context. Many properties of circumbinary discs around evolved post-asymptotic giant branch (post-AGB) binary systems are similar to those of protoplanetary discs around young stars. The deficits of near-infrared (near-IR) flux in the spectral energy distributions (SEDs) of these systems hints towards large dust-free cavities that are reminiscent of transition discs as are commonly observed around young stars.
Aims. We aim to assess the size of the inner rim of six post-AGB binary systems with lack in the near-IR like this. We used resolved mid-infrared (mid-IR) high-angular resolution observations of VLTI/MATISSE and VLTI/MIDI. The inner rim of only one such system was previously resolved. We compared these inner rim sizes to five systems with available MATISSE data that were identified to host a disc starting at the dust sublimation radius.
Methods. We used geometric ring models to estimate the inner rim sizes, the relative flux contributions of the star, the ring, and an over-resolved emission, the orientation of the ring, and the spectral dependences of the components.
Results. We find that the inner dust rims of the targets with a lack of near-IR excess in their SEDs are ∼2.5 to 7.5 times larger than the theoretical dust sublimation radii, and inner rim sizes of the systems that do not show this deficit are similar to those of their theoretical dust sublimation radii. The physical radii of the inner rims of these transition discs around post-AGB binaries are 3–25 au, which are larger than the disc sizes inferred for transition discs around young stars with VLTI/MIDI. This is due to the higher stellar luminosities of post-AGB systems compared to young stars, implying larger dust sublimation radii and thus larger physical inner radii of the transition disc.
Conclusions. With mid-IR interferometric data, we directly confirm the transition disc nature of six circumbinary discs around post-AGB binary systems. Future observational and modelling efforts are needed to progress in our understanding of the structure, origin, and evolution of these transition discs.
Flux predictions in the transition region incorporating the effects from propagation of cosmic rays in the Galactic magnetic field
Authors: Kääpä Alex , Kampert Karl-Heinz , Becker Tjus Julia 
Published: April 2023

Galactic cosmic rays (GCRs) and (anisotropically injected) extragalactic cosmic rays (EGCRs) are both affected by the Galactic magentic field (GMF) on their voyage to Earth at energies pertaining to the transition from GCRs to EGCRs, such that their flux, composition and arrival directions are modified. GCRs increasingly leak from the Galaxy with rising energy, leading to a flux suppression. The flux modification imposed on EGCRs is more complex, but may exhibit (subtle) spectral breaks depending on the direction and nature of the injected anisotropy.
Using a full Monte Carlo approach with CRPropa and making realistic and minimal assumptions about the injected GCR and EGCR fluxes, we derive a prediction of the total all-particle flux in the transition region. We find that it cannot account for the flux measured by various cosmic ray experiments in this energy range. This calls for the need of an additional component to the flux in the transition region.