bluuebunny/crossref_metadata_2025_split huggingface dataset:
Identifying and prioritizing evidence needs in self-care interventions for sexual and reproductive health
Authors: Sedgh Gilda , Sorhaindo Annik 
Published: June 2023

BackgroundSelf-care as an extension of health care systems can increase access to care. The development of programs and generation of evidence to support self-care in sexual and reproductive health (SRH) is a relatively nascent field. We undertook a study to identify and prioritize evidence gaps for SRH self-care.MethodsWe used the CHNRI methodology and administered two online surveys to stakeholders affiliated with major self-care networks. The first survey was used to identify evidence gaps, and the second to prioritize them using predetermined criteria.ResultsWe received 51 responses to the first survey and 36 responses to the second. Many evidence gaps focused on awareness of and demand for self-care options and best mechanisms for supporting users of self-care with information, counseling and linkages to care.ConclusionA priority area of work ahead should be determining which aspects of the learning agenda reflect gaps in evidence and which reflect a need to effectively synthesize and disseminate existing evidence.
Edge computing at sea: high-throughput classification of in-situ plankton imagery for adaptive sampling
Authors: Schmid Moritz S. , Daprano Dominic , Damle Malhar M. , Sullivan Christopher M. , Sponaugle Su , Cousin Charles , Guigand Cedric , Cowen Robert K. 
Published: June 2023

The small sizes of most marine plankton necessitate that plankton sampling occur on fine spatial scales, yet our questions often span large spatial areas. Underwater imaging can provide a solution to this sampling conundrum but collects large quantities of data that require an automated approach to image analysis. Machine learning for plankton classification, and high-performance computing (HPC) infrastructure, are critical to rapid image processing; however, these assets, especially HPC infrastructure, are only available post-cruise leading to an ‘after-the-fact’ view of plankton community structure. To be responsive to the often-ephemeral nature of oceanographic features and species assemblages in highly dynamic current systems, real-time data are key for adaptive oceanographic sampling. Here we used the new In-situ Ichthyoplankton Imaging System-3 (ISIIS-3) in the Northern California Current (NCC) in conjunction with an edge server to classify imaged plankton in real-time into 170 classes. This capability together with data visualization in a heavy.ai dashboard makes adaptive real-time decision-making and sampling at sea possible. Dual ISIIS-Deep-focus Particle Imager (DPI) cameras sample 180 L s-1, leading to &gt;10 GB of video per min. Imaged organisms are in the size range of 250 µm to 15 cm and include abundant crustaceans, fragile taxa (e.g., hydromedusae, salps), faster swimmers (e.g., krill), and rarer taxa (e.g., larval fishes). A deep learning pipeline deployed on the edge server used multithreaded CPU-based segmentation and GPU-based classification to process the imagery. AVI videos contain 50 sec of data and can contain between 23,000 - 225,000 particle and plankton segments. Processing one AVI through segmentation and classification takes on average 3.75 mins, depending on biological productivity. A heavyDB database monitors for newly processed data and is linked to a heavy.ai dashboard for interactive data visualization. We describe several examples where imaging, AI, and data visualization enable adaptive sampling that can have a transformative effect on oceanography. We envision AI-enabled adaptive sampling to have a high impact on our ability to resolve biological responses to important oceanographic features in the NCC, such as oxygen minimum zones, or harmful algal bloom thin layers, which affect the health of the ecosystem, fisheries, and local communities.
Effects of sintering temperatures on the microstructure and mechanical properties of S390 powder metallurgy high-speed steel
Authors: Hu Qipeng , Wang Miaohui , Chen Yunbo , Si Zhen , Zhang Dongyue 
Published: June 2023

High-performance complex gear cutters and high-temperature bearings are just some of the applications where high-speed steels (HSSs) shine as a preferred material choice owing to their high hardness and outstanding wear resistance. In this work, the effects of sintering temperature on the microstructure and mechanical properties of S390 HSS prepared via spark plasma sintering (SPS) were investigated with a range of sintering temperatures from 930°C to 1,090°C, a uniaxial pressure of 50 MPa, and a holding time of 5 min. The results demonstrated that the improvements in density, hardness, red hardness, and three-point bending strength were confirmed as the sintering temperature increased from 930°C to 1,090°C. Temperature-induced microstructure evolutions were assessed for their contribution to property enhancement, such as powders with varying dimensions and carbides with diverse morphology and diameter. The specimen with the best comprehensive mechanical properties (67.1 HRC and 1,196.67 MPa) was prepared at 1,050°C via SPS. The wear coefficients decreased as the sintering temperature increased, and the observation results of worn surfaces of test pins confirmed that abrasive wear and oxidation wear dominated the wear experiments. Furthermore, the wear mechanism of dense and porous SPS HSS was illustrated and analyzed in terms of the debris and trapped carbides.
GB1a Ameliorates Ulcerative Colitis via Regulation of the NF-κB and Nrf2 Signaling Pathways in an Experimental Model
Authors: Yu Yuanyuan , Zheng Congmin , Lu Xu , Deng Changsheng , Xu Qin , Guo Wenfeng , Wu Qingye , Wang Qi , Liu Changhui , Huang Xinan , Song Jianping 
Published: June 2023

Graphical AbstractGB1a attenuates DSS-induced ulcerative colitis via remodeling of the NF-κB and Nrf2 signaling pathways.
Comparison between the influence of roxadustat and recombinant human erythropoietin treatment on blood pressure and cardio-cerebrovascular complications in patients undergoing peritoneal dialysis
Authors: Cheng Shuiqin , Zhou Tingting , Yu Le , Zhang Zhihong , Chen Yunmin , Zhang Man , Cui Jingjing , Yu Wenxin , Zhou Jian , Yu Yusheng 
Published: June 2023

IntroductionRoxadustat treatment in PD patients is equivalent to ESAs in increasing hemoglobin (Hb). But blood pressure, cardiovascular parameters, cardio-cerebrovascular complications and prognosis in the two groups before and after treatment has not been sufficiently discussed.MethodsSixty PD patients who were treated with roxadustat for renal anemia in our PD center recruited from June 2019 to April 2020 as roxadustat group. PD patients treated with rHuEPO were enrolled at a 1:1 ratio as rHuEPO group using the method of propensity score matching. Hb, blood pressure, cardiovascular parameters, cardio-cerebrovascular complications and prognosis were compared between the two group. All patients were followed up for at least 24 months.ResultsThere were no significant differences in baseline clinical data or laboratory values between roxadustat group and rHuEPO group. After 24 months of follow-up, there was no significant difference in Hb levels (p &gt; 0.05). There were no significant changes in blood pressure, or the incidence of nocturnal hypertension before and after treatment in roxadustat group (p &gt; 0.05), while blood pressure significantly increased in rHuEPO group after treatment (p &lt; 0.05). Compared with roxadustat group after follow-up, rHuEPO group had a higher incidence of hypertension, the levels of cardiovascular parameters were worse and cardio-cerebrovascular complications had a higher incidence (p &lt; 0.05). Cox regression analysis showed age, systolic blood pressure, fasting blood glucose, and rHuEPO use before baseline were risk factors for cardio-cerebrovascular complications in PD patients, while treatment with roxadustat was a protective factor for cardiovascular and cerebrovascular complications.ConclusionCompared with rHuEPO, roxadustat had less influence on blood pressure or cardiovascular parameters, and it was associated with a lower risk of cardio-cerebrovascular complications in patients undergoing PD. Roxadustat has a cardio-cerebrovascular protective advantage in PD patients with renal anemia.
The direct and mediating effects of cognitive impairment on the occurrence of falls: a cohort study based on community-dwelling old adults
Authors: Zhang Tianyi , Yang Cunmei , Shu Gangming , Gao Chang , Ma Hongying , Zou Lin , Zuo Jing , Liu Shaoni , Yan Jin , Hu Yixin 
Published: June 2023

BackgroundCognitive impairment has been reported to be associated with falls in older adults. However, the complex relationship among falls, cognitive impairment and its associated factors, which could be targeted with specific interventions, remains to be elucidated. This study aimed to examine the direct effects of cognitive impairment on falls, to identify the factors associated with cognitive impairment and to explore the mediation role of cognitive impairment in the association of fall with cognition related factors.MethodsThis 1-year follow-up cohort study enrolled old adults aged 60  years or over. Information about demographic and anthropometric characteristics, fall outcomes, function and nutritional status were collected through face-to-face interview. Cognitive function was evaluated by the Montreal Cognitive Assessment (MoCA). Multivariable regression analyses were used to test the association between cognitive impairment and falls and to identify the factors related to cognitive impairment. Additionally, we conduct causal mediation analyses to estimate the mediation effects of cognitive impairment in the pathways of fall occurrence.ResultsOf the 569 participants included in this study, 366 (64.32%) had cognitive impairment, 96 (16.87%) had fall history in the past 1  year, 81 (14.24%) experienced fall and 47 (8.26%) received treatment because of falling during the 1-year follow-up. The association between cognitive impairment and 1-year fall risk was confirmed after adjusting for multiple covariates [odds ratio (OR):2.03, 95% confidence interval (CI): 1.13–3.80]. IADL disability, depression and low grip strength were associated with a higher prevalence of cognitive impairment. While overweight, higher education and higher income level were found to be related to a lower risk of cognitive impairment. Among these associated factors, cognitive impairment mediated the positive association of falling with IADL ability and depression, and a negative relationship with education and income level.ConclusionOur study not only confirmed the direct influence of cognitive impairment on fall risk in older adults, but also suggested a mediating role that cognitive impairment played in the pathways of fall occurrence. Our finding could help develop more specific interventions for fall prevention.
Enactive artificial intelligence: subverting gender norms in human-robot interaction
Authors: Hipólito Inês , Winkle Katie , Lie Merete 
Published: June 2023

IntroductionThis paper presents Enactive Artificial Intelligence (eAI) as a gender-inclusive approach to AI, emphasizing the need to address social marginalization resulting from unrepresentative AI design.MethodsThe study employs a multidisciplinary framework to explore the intersectionality of gender and technoscience, focusing on the subversion of gender norms within Robot-Human Interaction in AI.ResultsThe results reveal the development of four ethical vectors, namely explainability, fairness, transparency, and auditability, as essential components for adopting an inclusive stance and promoting gender-inclusive AI.DiscussionBy considering these vectors, we can ensure that AI aligns with societal values, promotes equity and justice, and facilitates the creation of a more just and equitable society.
Induced pluripotent stem cell model revealed impaired neurovascular interaction in genetic small vessel disease Cerebral Autosomal Dominant Arteriopathy with Subcortical Infarcts and Leukoencephalopathy
Authors: Zhang Wenjun , Zhao Xiangjun , Qi Xuewei , Kimber Susan J. , Hooper Nigel M. , Wang Tao 
Published: June 2023

IntroductionCerebral Autosomal Dominant Arteriopathy with Subcortical Infarcts and Leukoencephalopathy (CADASIL) is the most common genetic small vessel disease caused by variants in the NOTCH3 gene. Patients with CADASIL experience recurrent strokes, developing into cognitive defect and vascular dementia. CADASIL is a late-onset vascular condition, but migraine and brain MRI lesions appear in CADASIL patients as early as their teens and twenties, suggesting an abnormal neurovascular interaction at the neurovascular unit (NVU) where microvessels meet the brain parenchyma.MethodsTo understand the molecular mechanisms of CADASIL, we established induced pluripotent stem cell (iPSC) models from CADASIL patients and differentiated the iPSCs into the major NVU cell types including brain microvascular endothelial-like cells (BMECs), vascular mural cells (MCs), astrocytes and cortical projection neurons. We then built an in vitro NVU model by co-culturing different neurovascular cell types in Transwells and evaluated the blood brain barrier (BBB) function by measuring transendothelial electrical resistance (TEER).ResultsResults showed that, while the wild-type MCs, astrocytes and neurons could all independently and significantly enhance TEER of the iPSC-BMECs, such capability of MCs from iPSCs of CADASIL patients was significantly impaired. Additionally, the barrier function of the BMECs from CADASIL iPSCs was significantly decreased, accompanied with disorganized tight junctions in iPSC-BMECs, which could not be rescued by the wild-type MCs or sufficiently rescued by the wild-type astrocytes and neurons.DiscussionOur findings provide new insight into early disease pathologies on the neurovascular interaction and BBB function at the molecular and cellular levels for CADASIL, which helps inform future therapeutic development.
Altered metabolic-functional coupling in the epileptogenic network could predict surgical outcomes of mesial temporal lobe epilepsy
Authors: Yuan Siyu , Huang Hui , Cai Bingyang , Li Jiwei , Zhang Miao , Luo Jie 
Published: June 2023

ObjectiveTo investigate the relationship between glucose metabolism and functional activity in the epileptogenic network of patients with mesial temporal lobe epilepsy (MTLE) and to determine whether this relationship is associated with surgical outcomes.Methods18F-FDG PET and resting-state functional MRI (rs-fMRI) scans were performed on a hybrid PET/MR scanner in 38 MTLE patients with hippocampal sclerosis (MR-HS), 35 MR-negative patients and 34 healthy controls (HC). Glucose metabolism was measured using 18F-FDG PET standardized uptake value ratio (SUVR) relative to cerebellum; Functional activity was obtained by fractional amplitude of low-frequency fluctuation (fALFF). The betweenness centrality (BC) of metabolic covariance network and functional network were calculated using graph theoretical analysis. Differences in SUVR, fALFF, BC and the spatial voxel-wise SUVR-fALFF couplings of the epileptogenic network, consisting of default mode network (DMN) and thalamus, were evaluated by Mann-Whitney U test (using the false discovery rate [FDR] for multiple comparison correction). The top ten SUVR-fALFF couplings were selected by Fisher score to predict surgical outcomes using logistic regression model.ResultsThe results showed decreased SUVR-fALFF coupling in the bilateral middle frontal gyrus (PFDR = 0.0230, PFDR = 0.0296) in MR-HS patients compared to healthy controls. Coupling in the ipsilateral hippocampus was marginally increased (PFDR = 0.0802) in MR-HS patients along with decreased BC of metabolic covariance network and functional network (PFDR = 0.0152; PFDR = 0.0429). With Fisher score ranking, the top ten SUVR-fALFF couplings in regions from DMN and thalamic subnuclei could predict surgical outcomes with the best performance being a combination of ten SUVR-fALFF couplings with an AUC of 0.914.ConclusionThese findings suggest that the altered neuroenergetic coupling in the epileptogenic network is associated with surgical outcomes of MTLE patients, which may provide insight into their pathogenesis and help with preoperative evaluation.
Transarterial chemoembolization with or without multikinase inhibitors for patients with unresectable hepatocellular carcinoma: a systematic review and meta-analysis of randomized controlled trials
Authors: Dong Han , Ge Dongfang , Qu Biao , Zhu Ping , Wu Qibiao , Wang Tianyun , Wang Jue , Li Zheng 
Published: June 2023

BackgroundRandomized controlled trials (RCTs) testing the combination therapy of transarterial chemoembolization (TACE) plus multikinase inhibitor (MKI) in patients with unresectable hepatocellular carcinoma (HCC) have yielded inconsistent results.MethodsIn this work, a systematic review and meta-analysis was performed to compare the TACE+MKI combination therapy versus TACE monotherapy in HCC patients with time to progression (TTP) adopted as primary outcome.ResultsA total of 10 RCTs comprising 2837 patients receiving combination therapy (TACE plus sorafenib, brivanib, orantinib or apatinib) were included. TACE+MKI significantly prolonged TTP (hazard ratio [HR] 0.74, 95% CI 0.62-0.89, p=0.001) versus TACE monotherapy. Subgroup analysis suggested MKI administration before TACE might be preferable to post-TACE MKI for TTP. TACE+MKI also increased objective response rate (ORR) (risk ratio [RR] 1.17, 95% CI 1.03-1.32, p=0.01), but failed to improve overall survival (OS) (HR 0.98, 95% CI 0.86-1.13, p=0.82) and progression-free survival (PFS) (HR 0.75, 95% CI 0.50-1.12, p=0.16). The incidence of any adverse event (AE) did not significantly differ between TACE+MKI and TACE groups (RR 1.17, 95% CI 0.96-1.42, p=0.01), while serious AEs showed significant difference (RR 1.41, 95% CI 1.26-1.59, p&lt;0.0001). Nevertheless, these AEs showing significant difference were mainly associated with MKI toxicities rather than TACE. ConclusionsTACE+MKI combination therapy improved TTP and ORR but not OS and PFS in patients with unresectable HCC. Further high-quality trials are needed to verify these clinical benefits, and our findings could be very informative for future trial design.
Tumor-associated M2 macrophages in the immune microenvironment influence the progression of renal clear cell carcinoma by regulating M2 macrophage-associated genes
Authors: Zhang Xiaoxu , Sun Yang , Ma Yushuo , Gao Chengwen , Zhang Youzhi , Yang Xiaokun , Zhao Xia , Wang Wei , Wang Lisheng 
Published: June 2023

BackgroundRenal clear cell carcinoma (RCC) has negative prognosis and high mortality due to its early diagnosis difficulty and early metastasis. Although previous studies have confirmed the negative progression of RCC is closely related to M2 macrophages in tumor-associated macrophages (TAMs), the specific mechanism is still unknownMethodsWe used immunofluorescence labeling and flow cytometry to detect the proportion of M2 macrophages in RCC tissues. And bioinformatics technique was used to obtain 9 M2 macrophage-related model genes, including SLC40A1, VSIG4, FUCA1, LIPA, BCAT1, CRYBB1, F13A, TMEM144, COLEC12. Using these genes, model formulas are constructed to devide samples into high and low risk groups, and then the overall survival (OS), progression-free survival (PFS) and Gene set enrichment analysis (GSEA) of the high and low risk groups were analyzed. Real-time quantitative polymerase chain reaction (RT-qPCR) was used to measure the expression of model genes between normal kidney tissue and RCC tissue, as well as between HK-2 cell and 786-O cell. Besides, we induced the M2 differentiation of THP-1 cell, and then co-cultured with the RCC cell 786-O in transwell to observe what effect M2 macrophages will cause on the invasion, migration and the expression of model genes of RCC.ResultsOur study demonstrated M2 macrophages in RCC was about 2 folds that of normal renal tissue (P&lt;0.0001) and M2 macrophages affected the prognosis of patients with RCC by affecting the co-expressed genes, which were mainly enriched in immune-related pathways. The results of in vitro experiments showed that in RCC tissues and 786-O cells, the model gene FUCA1 was down-regulated, and SLC40A1, VSIG4, CRYBB1 and LIPA were up-regulated. Besides, the results of co-culture showed that after 786-O co-culture with M2 macrophages, the ability of migration and invasion was promoted and the expressions of FUCA1, SLC40A1, VSIG4, CRYBB1, LIPA and TMEM144 were all up-regulated.ConclusionThe proportion of tumor-associated M2 macrophages in RCC tissues is upregulated, and M2 macrophages promote the progression of RCC by regulating the expression of SLC40A1, VSIG4, FUCA1, LIPA, BCAT1, CRYBB1, F13A, TMEM144, COLEC12 genes, thereby affecting the prognosis of patients with RCC.
Sulfatinib, a novel multi-targeted tyrosine kinase inhibitor of FGFR1, CSF1R, and VEGFR1–3, suppresses osteosarcoma proliferation and invasion via dual role in tumor cells and tumor microenvironment
Authors: Liao Song , Li Jianxiong , Gao Song , Han Yuchen , Han Xinli , Wu Yanan , Bi Jingyou , Xu Meng , Bi Wenzhi 
Published: June 2023

IntroductionTumor progression is driven by intrinsic malignant behaviors caused by gene mutation or epigenetic modulation, as well as crosstalk with the components in the tumor microenvironment (TME). Considering the current understanding of the tumor microenvironment, targeting the immunomodulatory stromal cells such as cancer-associated fibroblasts (CAFs) and tumor-associated macrophages (TAMs) could provide a potential therapeutic strategy. Here, we investigated the effect of sulfatinib, a multi-targeted tyrosine kinase inhibitor (TKI) of FGFR1, CSF1R, and VEGFR1–3, on the treatment of osteosarcoma (OS).MethodsIn vitro, the antitumor effect was tested by clony formation assay and apoptosis assay.The inhibition of tumor migration and invasion was detected by Transwell assay, and the de-polarization of macrophage was detected by flow cytometry.In vivo, subcutaneous and orthotopic tumor models were established to verify antitumor effect, and the underlying mechanism was verified by immunohistochemistry(IHC), immunofluorescence(IF) and flow cytometry.ResultsSulfatinib suppressed OS cell migration and invasion by inhibiting epithelial–mesenchymal transition (EMT) by blocking the secretion of basic fibroblast growth factor (bFGF) in an autocrine manner. In addition, it regulated immune TME via inhibition of the migration of skeletal stem cells (SSCs) to the TME and the differentiation from SSCs to CAFs. Moreover, sulfatinib can suppress OS by modulation of the TME by inhibiting M2 polarization of macrophages. Systemic treatment of sulfatinib can reduce immunosuppression cells M2-TAMs, Tregs, and myeloid-derived suppressor cells (MDSCs) and increase cytotoxic T-cell infiltration in tumors, the lungs, and the spleens.DiscussionOur preclinical experiments have shown that sulfatinib can inhibit the proliferation, migration, and invasion of OS by playing a dual role on tumor cells and the tumor microenvironment simultaneously and systematically reverse immunosuppression to immune activation status, which could be translated into clinical trials.