bluuebunny/crossref_metadata_2025_split huggingface dataset:
Prevalence and risk factors of antibodies towards HLA Class I and Class II in Malaysian renal transplant candidates
Authors: Khairul-Fahmy Norfarhana , Ismail Jamiila , Koay Bee Tee , Md-Zakariah Muhammad Zhafri , Mansor Salawati , Zulkifli Nordalila , Mat-Ali Siti Fatimah , Mohamed Rozinah , Mustafa Norhazlin , Arip Masita 
Published: February 2023

AbstractAntibody-mediated rejection (AMR) still persists as the major hurdle towards successful renal allograft survival. This paper aims to report on the HLA antibody landscape of renal transplant candidates in Malaysia. A total of 2,219 adult samples from 2016 to 2019 were analysed for anti-HLA antibodies using solid-phase assay. Our findings highlight the prevalence and risk factors for antibodies against HLA antigens in renal transplant settings, which could be beneficial for selecting compatible recipients from deceased organ donors. To the best of our knowledge, this study is the first to demonstrate that ethnic Malay and Chinese showed significantly higher prevalence of anti-HLA antibodies. Based on our multivariate analysis: (i) female gender was associated with higher risk for panel reactive antibodies (PRAs) against Class I, Class II, and Class I and II (p < 0.001); (ii) older patients (≥ 38 years old) were associated with higher risk of positivity against Class I, Class II and Class I and II (p < 0.001); (iii) Malays showed significant association with Class II antibodies (p = 0.035); Chinese patients presented with higher risk of PRA positivity against Class II (p < 0.001) and Class I and II (p = 0.01); Indians were significantly associated with higher risk of HLA antibody sensitization against Class I (p = 0.022), Class II (p = 0.026) and Class I and II (p = 0.05). Thus, our findings suggested that female gender, older age (≥ 38 years old) and ethnicity may serve as independent risk factors for HLA antibody sensitization in adult renal transplant candidates.
Sociodemographic differences in Covid-19 vaccine uptake in Denmark: a nationwide register-based cohort study
Authors: Gram Mie Agermose , Moustsen-Helms Ida Rask , Valentiner-Branth Palle , Emborg Hanne-Dorthe 
Published: February 2023

Abstract
Background
Covid-19 vaccination is the main strategy to reduce SARS-CoV-2 transmission, mortality and morbidity. This study aimed to examine sociodemographic differences in Covid-19 vaccine uptake among all individuals invited for Covid-19 vaccination in Denmark.

Methods
This study was designed as a nationwide register-based cohort study. The study population included all Danish residents aged 12 years or above in Denmark between December 27, 2020 and October 20, 2021. Individuals who died during the study period before receiving an invitation for Covid-19 vaccination were excluded. Associations between sociodemographic factors and Covid-19 vaccination uptake were analyzed using logistic regression models adjusting for age, sex, immigration status, educational level, disposable income and history of SARS-CoV-2 infection.

Results
The study population included 5,164,558 individuals. The overall vaccination coverage was 87.1% by October 20, 2021. In the full adjusted logistic regression models, the highest ORs for non-vaccination were observed among individuals aged 12–24 years (OR: 8.99 (95% CI: 8.76–9.23)), descendants of non-western immigrants (OR: 5.26 (95% CI: 5.18–5.33)), individuals who never had a PCR-test performed (OR: 2.93 (95% CI: 2.90–2.96)), individuals with primary school as highest completed educational level (OR: 2.87 (95% CI: 2.83–2.91)) and individuals with disposable income < 33,605 EUR (OR: 3.72 (95% CI: 3.52–3.93)).

Conclusion
Overall, the Covid-19 vaccine uptake was high in Denmark. However, large sociodemographic differences in the vaccine uptake exist. The youngest age groups had the lowest vaccination coverage. Furthermore, the impact of the sociodemographic factors was more pronounced in the youngest age groups. The identified determinants may be used to design policies to help maximize the vaccination coverage.
Evidence for recency of practice standards for regulated health practitioners in Australia: a systematic review
Authors: Main Penelope Ann Elizabeth , Anderson Sarah 
Published: February 2023

Abstract
Background
Health practitioner regulators throughout the world use registration standards to define the requirements health practitioners need to meet for registration. These standards commonly include recency of practice (ROP) standards designed to ensure that registrants have sufficient recent practice in the scope in which they intend to work to practise safely. As the ROP registration standards for most National Boards are currently under review, it is timely that an appraisal of current evidence be carried out.

Methods
A systematic review was conducted using databases (including MEDLINE, EMBASE, PsycInfo, and CINAHL), search engines, and a review of grey literature published between 2015 and April 2022. Publications included in the review were assessed against the relevant CASP checklist for quantitative studies and the Joanna Briggs Institute checklist for analytical cross-sectional studies.

Results
The search yielded 65 abstracts of which 12 full-text articles met the inclusion criteria. Factors that appear to influence skills retention include the length of time away from practice, level of previous professional experience and age, as well as the complexity of the intervention. The review was unable to find a clear consensus on the period of elapsed time after which a competency assessment should be completed.

Conclusions
Factors that need to be taken into consideration in developing ROP standards include length of time away from practice, previous experience, age and the complexity of the intervention, however, there is a need for further research in this area.
Metabolic classifications of renal cell carcinoma reveal intrinsic connections with clinical and immune characteristics
Authors: Li Le , Chao Zheng , Waikeong Un , Xiao Jun , Ge Yue , Wang Yanan , Xiong Zezhong , Ma Sheng , Wang Zhihua , Hu Zhiquan , Zeng Xing 
Published: February 2023

Abstract
Background
Kidney cancer undergoes a dramatic metabolic shift and has demonstrated responsiveness to immunotherapeutic intervention. However, metabolic classification and the associations between metabolic alterations and immune infiltration in Renal cell carcinoma still remain elucidative.

Methods
Unsupervised consensus clustering was conducted on the TCGA cohorts for metabolic classification. GESA, mRNAsi, prognosis, clinical features, mutation load, immune infiltration and differentially expressed gene differences among different clusters were compared. The prognosis model and nomograms were constructed based on metabolic gene signatures and verified using external ICGC datasets. Immunohistochemical results from Human Protein Atlas database and Tongji hospital were used to validate gene expression levels in normal tissues and tumor samples. CCK8, apoptosis analysis, qPCR, subcutaneously implanted murine models and flowcytometry analysis were applied to investigate the roles of ACAA2 in tumor progression and anti-tumor immunity.

Results
Renal cell carcinoma was classified into 3 metabolic subclusters and the subcluster with low metabolic profiles displayed the poorest prognosis, highest invasiveness and AJCC grade, enhanced immune infiltration but suppressive immunophenotypes. ACAA2, ACAT1, ASRGL1, AKR1B10, ABCC2, ANGPTL4 were identified to construct the 6 gene-signature prognosis model and verified both internally and externally with ICGC cohorts. ACAA2 was demonstrated as a tumor suppressor and was associated with higher immune infiltration and elevated PD-1 expression of CD8+ T cells.

Conclusions
Our research proposed a new metabolic classification method for RCC and revealed intrinsic associations between metabolic phenotypes and immune profiles. The identified gene signatures might serve as key factors bridging tumor metabolism and tumor immunity and warrant further in-depth investigations.
Prognostic value of baseline 18F-FDG PET/CT in patients with esophageal squamous cell carcinoma treated with definitive (chemo)radiotherapy
Authors: Xia Lianshuang , Li Xiaoxu , Zhu Jie , Gao Zhaisong , Zhang Ju , Yang Guangjie , Wang Zhenguang 
Published: February 2023

Abstract
Purpose
To investigate the prognostic value of baseline 18F-FDG PET/CT in patients with esophageal squamous cell carcinoma (ESCC) treated with definitive (chemo)radiotherapy.

Methods
A total of 98 ESCC patients with cTNM stage T1-4, N1-3, M0 who received definitive (chemo)radiotherapy after 18F-FDG PET/CT examination from December 2013 to December 2020 were retrospectively analyzed. Clinical factors included age, sex, histologic differentiation grade, tumor location, clinical stage, and treatment strategies. Parameters obtained by 18F-FDG PET/CT included SUVmax of primary tumor (SUVTumor), metabolic tumor volume (MTV), total lesion glycolysis (TLG), SUVmax of lymph node (SUVLN), PET positive lymph nodes (PLNS) number, the shortest distance between the farthest PET positive lymph node and the primary tumor in three-dimensional space after the standardization of the patient BSA (SDmax(LN-T)). Univariate and multivariate analysis was conducted by Cox proportional hazard model to explore the significant factors affecting overall survival (OS) and progression-free survival (PFS) in ESCC patients.

Results
Univariate analysis showed that tumor location, SUVTumor, MTV, TLG, PLNS number, SDmax (LN-T) were significant predictors of OS and tumor location, and clinical T stage, SUVTumor, MTV, TLG, SDmax (LN-T) were significant predictors of PFS (all p < 0.1). Multivariate analysis showed that MTV and SDmax (LN-T) were independent prognostic factors for OS (HR = 1.018, 95% CI 1.006–1.031; p = 0.005; HR = 6.988, 95% CI 2.119–23.042; p = 0.001) and PFS (HR = 1.019, 95% CI 1.005–1.034; p = 0.009; HR = 5.819, 95% CI 1.921–17.628; p = 0.002). Combined with independent prognostic factors MTV and SDmax (LN-T), we can further stratify patient risk.

Conclusions
Before treatment, 18F-FDG PET/CT has important prognostic value for patients with ESCC treated with definitive (chemo)radiotherapy. The lower the value of MTV and SDmax (LN-T), the better the prognosis of patients.
Early versus delayed treatment of lateral condylar fracture of the humerus with > 2 mm displacement in children: a retrospective study
Authors: Wang Biao , Gao Rongxuan , Li Zhenwei , Guo Zhanhao , Ji Zejuan , Li Weili , Sun Keming 
Published: February 2023

Abstract
Background
The purpose of this study was to investigate the clinical and functional outcomes of early versus delayed treatment of pediatric lateral condylar fractures of the humerus with a displacement greater than 2 mm.

Methods
Sixty-seven children treated surgically at our hospital from March 2016 to September 2021 for lateral condylar fracture of the humerus with displacement > 2 mm were retrospectively analyzed. The children were divided into two groups where early surgery consisted of patients being operated on within 24-h post-injury (n = 36) and delayed surgery consisted of children operated after 24-h post-injury (n = 31). Clinical and functional results were compared between the two groups.

Results
There were no significant differences between the two groups in terms of operation time, blood loss and incidences of perioperative complications. However, mean length of incision was significantly greater (P < 0.0001) in the delayed treatment group (5.68 ± 1.08 cm) compared to the early treatment group (3.89 ± 0.82 cm). No differences were found in functional outcomes, consisting of the Baumann angle of the affected limb, the carrying angle, Mayo Elbow Performance Score, and Flynn’s criteria at final follow-up.

Conclusions
Delay in surgery for more than 24 h after injury does not influence the clinical and functional results for lateral condylar fracture of the humerus with displacement > 2 mm in children. However, delayed open reduction and pinning may increase the incision length possibly due to increased edema.
Areas of improvement in the medical care of SMA: evidence from a nationwide patient registry in Germany
Authors: Leibrock Berenike , Landfeldt Erik , Hussong Justine , Huelle Tabea , Mattheus Hannah , Thiele Simone , Walter Maggie C. , Zemlin Michael , Moehler Eva , Dillman Ullrich , Abner Sophia , Flotats-Bastardas Marina 
Published: February 2023

Abstract
Background
Management and treatment of spinal muscular atrophy (SMA) has changed in recent years due to the introduction of novel transformative and potentially curative therapies resulting in the emergence of new disease phenotypes. Yet, little is known about the uptake and impact of these therapies in real-world clinical practice. The objective of this study was to describe current motor function, need of assistive devices, and therapeutic and supportive interventions provided by the healthcare system, as well as the socioeconomic situation of children and adults with different SMA phenotypes in Germany. We conducted a cross-sectional, observational study of German patients with genetically confirmed SMA identified and recruited via a nationwide SMA patient registry (www.sma-register.de) within the TREAT-NMD network. Study data was recorded directly from patient-caregiver pairs through a study questionnaire administered online via a dedicated study website.

Results
The final study cohort consisted of 107 patients with SMA. Of these, 24 were children and 83 adults. In total, about 78% of all participants were taking medication for SMA (predominantly nusinersen and risdiplam). All children with SMA1 were able to sit and 27% of children with SMA2 were able to stand or walk. Impaired upper limb function, scoliosis and bulbar dysfunction were observed more frequently in patients with reduced lower limb performance. Physiotherapy, occupational therapy, and speech therapy, as well as the use of cough assists were less common than indicated by care guidelines. Family planning and educational and employment status appear to be related to motor skill impairment.

Conclusions
We show that the natural history of disease has changed in Germany following improvements in SMA care and the introduction of novel therapies. Yet, a non-trivial proportion of patients remain untreated. We also identified considerable limitations in rehabilitation and respiratory care, as well as low labour-market participation among adults with SMA, calling for action to improve the current situation.
Sharp bounds for Hardy-type operators on mixed radial-angular central Morrey spaces
Authors: Wei Mingquan , Yan Dunyan 
Published: February 2023

AbstractBy using the rotation method, a sharp bound for an n-dimensional Hardy operator on mixed radial-angular central Morrey spaces is obtained. Furthermore, a sharp weak-type estimate for an n-dimensional Hardy operator on mixed radial-angular central Morrey spaces is established. In addition, we also calculate the sharp constant for an n-dimensional m-linear Hardy operator from product mixed radial-angular central Morrey spaces to mixed radial-angular weak central Morrey spaces. Since mixed radial-angular central Morrey spaces are generalizations of both central Morrey spaces and mixed radial-angular spaces, the main theorems in this paper extend various existing results to a more general setting.
Realizing sensations: analyzing Paul Cezanne’s watercolors and assessing their light sensitivity with microfade testing
Authors: Haddad Abed , Neufeld Laura , Martins Ana 
Published: February 2023

Abstract
The exhibition Cézanne Drawing at The Museum of Modern Art (MoMA) brought together an exceptional group of works on paper from public and private collections across the globe. Recognizing the inherent light sensitivity of both the paper and watercolors, controlling, and tracking light exposure was central to the exhibition planning. This concern also led to a systematic study of three watercolors in the museum’s collection, Foliage (1895), Study of Trees (1895), and Mont Sainte-Victoire (1902-06), to characterize the watercolor paints used by Cezanne in these works and their sensitivity to light exposure, and to better understand the condition of the drawings based on the palette’s chemistry. Examination and analysis were undertaken non-invasively and micro-invasively with the following techniques: Infrared Reflectography (IRR), Ultraviolet Fluorescence Photography (UVF), Raman and surface-enhanced Raman (SERS) spectroscopies in addition to X-Ray fluorescence analysis on small spots and large areas using portable (p-XRF) and XRF scanning, respectively. The palette for these three watercolor drawings includes lead white, bone black, vermilion, yellow ochre, chrome yellow, emerald green, viridian, cobalt blue, and synthetic alizarin and carmine lakes. Microfade testing (MFT) was performed on the paper support and spots with each identified pigment, and the data acquired was evaluated both for color change (ΔE00) and rate of color change (

$$\frac{\partial \Delta {E}_{00}}{\partial t}$$

∂
Δ

E
00

∂
t

). Together these techniques inform the future display and loan of these and similarly fugitive watercolors in Cézanne’s oeuvre.
Factors associated with wasting and stunting among children aged 06–59 months in South Ari District, Southern Ethiopia: a community-based cross-sectional study
Authors: Toma Temesgen Mohammed , Andargie Kassahun Tamene , Alula Rahel Abera , Kebede Bahiru Mulatu , Gujo Mintesinot Melka 
Published: February 2023

Abstract
Background
Child undernutrition is a major public health problem in Ethiopia despite different nutrition-specific and sensitive interventions implemented by the government. However, evidence regarding the burden and responsible factors is limited in the South Ari district. Hence, this study aimed to assess factors associated with wasting and stunting among children aged 06–59 months in the South Ari district, Southern Ethiopia, 2021.

Methods
A community-based cross-sectional study was conducted from March 11 to April 11, 2021, among 717 households with at least one child aged 06–59 months. Anthropometric measurements were taken using standard procedures and were converted to height for age Z score and weight-for-height using WHO Anthro software Version 3.2.2. Data were checked and entered into Epi-Data Version 3.1 and analyzed using SPSS Version 25.0. Binary logistic regression analysis was fitted to identify predictors of wasting and stunting. A p-value < 0.05 was used to declare statistical significance.

Result
The prevalence of wasting and stunting among children aged 06–59 months in the district was 9.1% (95% CI: 7.07%, 11.41%) and 59.97% (95% CI: 56.28%, 63.58%), respectively. Family size (8 and above) (AOR = 3.03, 95% CI: 1.31, 7.03), family size (5 to 7) (AOR = 2.05, 95% CI: 1.11, 3.81), poor and medium wealth index (AOR = 3.69, 95% CI: 1.65, 8.26) and (AOR = 2.29, 95% CI: 1.01, 5.16), insufficient maternal knowledge on child feeding practice (AOR = 2.58, 95% CI: 1.31, 5.07), presence of diarrhea in the past two weeks (AOR = 2.05, 95% CI: 1.10, 3.85), non-exclusive breastfeeding (AOR = 2.65, 95% CI: 1.51, 4.65), and birth interval of < 24 months (AOR = 4.49, 95% CI: 2.40, 8.37) were factors significantly associated with wasting. Whereas, children in the age group of 24–59 months (AOR = 2.24, 95% CI: 1.58, 3.16), non-exclusive breastfeeding (AOR = 1.81, 95% CI: 1.24, 2.65), birth interval of fewer than 24 months (AOR = 1.54, 95% CI: 1.11, 2.14), sub-optimal child dietary diversity score (AOR = 1.59, 95% CI: 1.14, 2.22), being a non-beneficiary of productive safety-net program (AOR = 1.91, 95% CI: 1.24, 2.95), and household food insecurity (AOR = 2.60, 95% CI: 1.86, 3.64) were factors significantly associated with stunting.

Conclusions
Wasting and stunting were found to be key public health problems in the South Ari District. Hence, further interventions should consider strategies to enhance household food security and integration of productive safety net programs with primary health care services. Rigorous work is required in advocating and strengthening the provision of family planning services, child care, and integrated management of common childhood illnesses. Moreover, behavioral change communication is highly demanded to improve child feeding practices.
Development of novel betabaculovirus (HycuGV-Hc1) as a biopesticide (HycuGV-TR61) and its efficacy on the fall webworm, Hyphantria cunea Drury (Lepidoptera: Erebidae) larvae
Authors: Bayramoglu Zeynep , Gencer Donus , Demir Ismail 
Published: February 2023

AbstractBackgroundThe aim of this study was to develop an oil formulation from a local betabaculovirus.Hyphantria cuneaDrury (Lepidoptera: Erebidae) granulovirus (HycuGV-Hc1) was isolated from the infected larvae to test its efficacy on the pest. The oil formulation was prepared by mixing the viral suspension with sunflower oil and some adjuvants and named HycuGV-TR61. Crude virus and the formulations were carried out on third instarH. cunealarvae using 1 × 104–8OBs /ml concentrations and were exposed to temperatures (28, 35, and 42 °C) and UV-B light at different periods (0, 1, 3, 5 h).ResultsThe mortality rate, which was 50% at the lowest concentration (1 × 104OB/ml), reached 99.86% at the highest concentration (1 × 108OB/ml). LC50values of fresh and old forms were calculated as 0.64 × 104and 0.87 × 104OBs/ml, respectively. Application of shelf life showed that there was non-significant change in the pathogenic activity of the formulation with time. In the experiments, it was observed that the activity decreased as the temperature and time of exposure increased. Significantly difference in larval mortality was observed when fresh and old formulations were exposed to 0, 1, 3 and 5 h to UV-B, (old: 96.7, 86, 80 and 60%; fresh: 97.1, 90, 85 and 62%, respectively).ConclusionsThe results revealed superior aspects of HycuGV-TR61, which was developed as a local viral biopesticide, its resistance to abiotic factors and its potential to be used in pest control.