bluuebunny/crossref_metadata_2025_split huggingface dataset:
Selective extraction of apixaban from plasma by dispersive solid‐phase microextraction using magnetic metal organic framework combined with molecularly imprinted polymer nanocomposite
Authors: Fathi Ali Akbar , Afshar Mogaddam Mohammad Reza , Sorouraddin Saeed Mohammad , Farajzadeh Mir Ali , Mohebbi Ali 
Published: June 2023

This research aims to synthesize a specific and efficient sorbent to use in the extraction of apixaban from human plasma samples and its determination by high‐performance liquid chromatography‐tandem mass spectrometry. High specific surface area of metal‐organic framework, magnetic property of iron oxide nanoparticles, selectively of molecular imprinted polymer toward the analyte, and the combination of dispersive solid‐phase extraction method with a sensitive analysis system provided an efficient analytical method. In this study, first, a molecularly imprinted polymer combined with magnetic metal organic framework nanocomposite was prepared and then characterized using different techniques. Then the sorbent particles were used for selective extraction of the analyte from plasma samples. The efficiency of the method was improved by optimizing effective parameters. According to the validation results, wide linear range (1.02–200 ng mL−1), acceptable coefficient of determination (0.9938), low limit of detection (0.32 ng mL−1) and limit of quantification (1.02 ng mL−1), high extraction recovery (78%), and good precision (relative standard deviations ≤ 2.9% for intra‐ (n = 6) and interday (n = 6) precisions) were obtainable using the proposed method. These outcomes showed the high potential of the proposed method for screening apixaban in the human plasma samples.
Achievement and maintenance of therapeutic response to brodalumab in patients with moderate‐to‐severe plaque psoriasis: An Italian, observational, retrospective and prospective study (BRIGHT study)
Authors: Dapavo Paolo , Fabbrocini Gabriella , Campione Elena , Giofrè Claudia , Balato Anna , Potenza Concetta , Dastoli Stefano , Malagoli Piergiorgio , Offidani Annamaria , Bardazzi Federico , Peris Ketty , Pella Paolo , De Pasquale Rocco , Bonifati Claudio , Giacchetti Alfredo , Pallotta Sabatino , Congedo Maurizio , Amerio Paolo , Fargnoli Maria Concetta , Calzavara Pinton Piergiacomo , Venturini Marina ,   the BRIGHT Study Group
Published: June 2023

AbstractBackgroundBrodalumab, a fully human anti‐interleukin (IL)‐17 monoclonal antibody that blocks IL‐17RA, is approved in Europe for the treatment of adult patients with moderate‐to‐severe plaque psoriasis who are candidates for systemic treatment.ObjectivesThis study evaluated the achievement and maintenance of therapeutic response for 1 year in psoriasis patients treated with brodalumab in the Italian clinical practice.MethodsReal‐world, multicenter, observational, retrospective and prospective study. The retrospective phase ranged from enrolment visit to initiation of brodalumab (12 ± 4 weeks before). The prospective phase ranged from enrolment to a routine follow‐up visit set after 52 ± 4 weeks.ResultsOne hundred eighty‐four patients were eligible and 164 completed the observation period (median 11.9 months; Q1–Q3: 11.4–12.3). At enrolment, the mean duration of disease was 13.9 years (standard deviation; 13.3), 94% of patients (N = 172) had ≥1 clinical manifestation of psoriasis, mainly erythema and itching, and 95.6% had received ≥1 antipsoriatic treatment before brodalumab. Patients who reached an absolute Psoriasis Area and Severity Index (PASI) score ≤3 at week 12 and maintained it ≤3 through week 52 were 97 (64.7%). At week 12, 72.7% of patients achieved PASI 75, 54.5% PASI 90 and 42.0% PASI 100. At Week 52, 92.9% of patients achieved PASI 75, 84.4% PASI 90 and 61.7% PASI 100. A static Physician's Global Assessment (sPGA) score = 0 was obtained by 55.0% of patients after 12 weeks of brodalumab and by 77.0% after 52 weeks. A Dermatology Life Quality Index ≤1 was reported by 71.9% of patients after 12 weeks and by 89.9% after 52 weeks of treatment. No significant outcome differences were shown among patient subgroups defined by previous antipsoriatic treatments (none, systemic other than biologics or biologic) at either brodalumab initiation or any of the subsequent study visits.ConclusionsIn this real‐world setting, brodalumab was rapidly effective on skin lesions and quality of life both in biologic‐naïve and biologic‐experienced moderate‐to‐severe psoriasis patients, with a favourable safety profile.
Height‐Gradiently‐Tunable Nanostructure Arrays by Grayscale Assembly Nanofabrication for Ultra‐realistic Imaging
Authors: Geng Guangzhou , Pan Ruhao , Li Chensheng , Zheng Ruixuan , Liu Yunan , Zhang Zhongshan , Gu Changzhi , Li Junjie 
Published: June 2023

AbstractDue to the limitation of nanofabrication technology, nanostructured arrays and devices are usually of equal height, and it is difficult to change simultaneously its height in the Z direction at X–Y direction regulation. However, the tunable height as an indispensable part of structural spatial freedom control is very conducive to the functional expansion and innovation of nanodevices. Here, a grayscale assembled fabrication method is developed by combining e‐beam lithography with atomic layer deposition and transfer technology, and a wide range of the height gradient of nanostructures can be realized in the same array and then applied in high‐quality images. Following this strategy, ultra‐realistic relief‐nanostructured images with 256 grayscale levels are demonstrated, consisting of height‐varying nanopillars with 100 nm in pixel size and 6.4×1010 dpi in resolution. Especially, multi‐dimensional structural color based on metasurfaces with C4 symmetric pixels is designed and prepared by height regulation nanofabrication, enabling a strong multi‐dimensional control ability in hue, saturation, and brightness of the structural color display with the single pixel of 1.5 µm in size. The as‐developed grayscale nanoconfigurations open a new approach for multidimensionally structural regulations, showing great applied potential not only in high‐resolution imaging but also in more advancednanophotonic devices.
Plasticization, Antiplasticization, and Fragility in Blends of Polyvinyl Butyral and Polyethylene Glycol Dimethyl Ether
Authors: Bartolotta Antonino , Carini Giuseppe , D'Angelo Giovanna , Federico Mauro , Romano Valentino , Di Marco Gaetano 
Published: June 2023

AbstractBlends of polyvinyl butyral (PVB) and polyethylene glycol dimethyl ether (PEGDME) with low molecular weight (= 250 g mol−1) show two calorimetric (for PEGDME content higher than 11% by weight) and mechanical glass transitions. Their substantial changes in the relative content of the two components are explained by considering the self‐concentrations of both polymers determined by the connectivity of their own chains. Increasing content of PEGDME lower the glass transition temperature Tg of PVB (the component with the highest Tg) by about 100 K, also causing a progressive and significant increase of its fragility (following the Angell's strong/fragile liquids classification scheme). Strengthening of polymer blends is observed in the fully glassy region, quite below the Tg's of both the components, with the storage modulus E′ becoming more than a factor 2 higher than that of neat PVB. The progressive increase of E′, without any change of slope, points to structural homogeneity of blends determined by compatibility of components, as a result of interchain interactions activated by the polar groups of both polymers. The present results reveal that PEGDME plasticizes PVB when the blend is in the fully rubbery state while acting as an anti‐plasticizer in the fully glassy state.
M1 macrophage‐derived exosomes promote autoimmune liver injury by transferring long noncoding RNA H19 to hepatocytes
Authors: Zhang Yongting , Hong Liang , Li Xuehui , Li Yuyu , Zhang Xujun , Jiang Jingjing , Shi Fan , Diao Hongyan 
Published: June 2023

AbstractExosomes mediate intercellular communication by transmitting active molecules. The function of long noncoding RNA (lncRNA) H19 in autoimmune liver injury is unclear. Concanavalin A (ConA)‐induced liver injury is well‐characterized immune‐mediated hepatitis. Here, we showed that lncRNA H19 expression was increased in the liver after ConA treatment, accompanied by increased exosome secretion. Moreover, injection of AAV‐H19 aggravated ConA‐induced hepatitis, with an increase in hepatocyte apoptosis. However, GW4869, an exosome inhibitor, alleviated ConA‐induced liver injury and inhibited the upregulation of lncRNA H19. Intriguingly, lncRNA H19 expression in the liver was significantly downregulated, after macrophage depletion. Importantly, the lncRNA H19 was primarily expressed in type I macrophage (M1) and encapsulated in M1‐derived exosomes. Furthermore, H19 was transported from M1 to hepatocytes via exosomes, and exosomal H19 dramatically induced hepatocytes apoptosis both in vitro and vivo. Mechanistically, H19 upregulated the transcription of hypoxia‐inducible factor‐1 alpha (HIF‐1α), which accumulated in the cytoplasm and mediated hepatocyte apoptosis by upregulating p53. M1‐derived exosomal lncRNA H19 plays a pivotal role in ConA‐induced hepatitis through the HIF‐1α–p53 signaling pathway. These findings identify M1 macrophage‐derived exosomal H19 as a novel target for the treatment of autoimmune liver diseases.
Genome‐wide identification of endogenous retrovirus elements and their active transcription in mink genome
Authors: Li Zheng , Wang Qing , Lv Na , Xu Guojin , Yang Xuemei , Zhu Baoli 
Published: June 2023

AbstractMammalian endogenous retroviruses (ERVs) are ancient retroviruses that have been integrated into genomes. ERVs were believed to be inactive until the discovery of ERV transcription in the mouse genome. However, the transcription level and function of ERV elements in mammalian genomes are not well understood. In this study, we performed the first genome‐wide scanning of ERV loci in the American mink (Neogale vison) genome (NeoERV) followed by transcriptomic analysis to detect actively transcribed NeoERV elements. A total of 365,791 NeoERV loci were identified, and161,205 (44%) of these loci were found to be actively transcribed based on transcriptomic data from three types of tissues (amygdala, trachea and lung). More than one third of the actively transcribed NeoERV loci were tissue‐specific. Furthermore, some of the active loci were associated with host gene transcription, and the level of NeoERV transcription was positively correlated with that of host genes, specifically when active loci were located in overlapped gene regions. An in‐depth analysis of the envelope protein coding env gene showed that, in general, its transcription level was higher than that of NeoERVs, which is believed to be associated with host immunity.
Deep learning‐based combination of [18F]‐FDG PET and CT images for producing pulmonary perfusion image
Authors: Gu Jiabing , Qiu Qingtao , Zhu Jian , Cao Qiang , Hou Zhen , Li Baosheng , Shu Huazhong 
Published: June 2023

AbstractBackgroundThe main application of [18F] FDG‐PET (18FDG‐PET) and CT images in oncology is tumor identification and quantification. Combining PET and CT images to mine pulmonary perfusion information for functional lung avoidance radiation therapy (FLART) is desirable but remains challenging.PurposeTo develop a deep‐learning‐based (DL) method to combine 18FDG‐PET and CT images for producing pulmonary perfusion images (PPI).MethodsPulmonary technetium‐99 m‐labeled macroaggregated albumin SPECT (PPISPECT), 18FDG‐PET, and CT images obtained from 53 patients were enrolled. CT and PPISPECT images were rigidly registered, and registration displacement was subsequently used to align 18FDG‐PET and PPISPECT images. The left/right lung was separated and rigidly registered again to improve the registration accuracy. A DL model based on 3D Unet architecture was constructed to directly combine multi‐modality 18FDG‐PET and CT images for producing PPI (PPIDLM). 3D Unet architecture was used as the basic architecture, and the input was expanded from a single‐channel to a dual‐channel to combine multi‐modality images. For comparative evaluation, 18FDG‐PET images were also used alone to generate PPIDLPET. Sixty‐seven samples were randomly selected for training and cross‐validation, and 36 were used for testing. The Spearman correlation coefficient (rs) and multi‐scale structural similarity index measure (MS‐SSIM) between PPIDLM/PPIDLPET and PPISPECT were computed to assess the statistical and perceptual image similarities. The Dice similarity coefficient (DSC) was calculated to determine the similarity between high‐/low‐ functional lung (HFL/LFL) volumes.ResultsThe voxel‐wise rs and MS‐SSIM of PPIDLM/PPIDLPET were 0.78 ± 0.04/0.57 ± 0.03, 0.93 ± 0.01/0.89 ± 0.01 for cross‐validation and 0.78 ± 0.11/0.55 ± 0.18, 0.93 ± 0.03/0.90 ± 0.04 for testing. PPIDLM/PPIDLPET achieved averaged DSC values of 0.78 ± 0.03/0.64 ± 0.02 for HFL and 0.83 ± 0.01/0.72 ± 0.03 for LFL in the training dataset and 0.77 ± 0.11/0.64 ± 0.12, 0.82 ± 0.05/0.72 ± 0.06 in the testing dataset. PPIDLM yielded a stronger correlation and higher MS‐SSIM with PPISPECT than PPIDLPET (p < 0.001).ConclusionsThe DL‐based method integrates lung metabolic and anatomy information for producing PPI and significantly improved the accuracy over methods based on metabolic information alone. The generated PPIDLM can be applied for pulmonary perfusion volume segmentation, which is potentially beneficial for FLART treatment plan optimization.
Small thiophene fluorophores in live cells promote protein self‐assembly into nanostructured fluorescent and electroactive microfibers
Authors: Palamà Ilaria Elena , Maiorano Gabriele , Barbarella Giovanna , Gigli Giuseppe 
Published: June 2023

AbstractThis review describes an unprecedented class of biocompatible protein‐based microfibers obtained by exploiting the ability of small thiophene fluorophores: (1) to be spontaneously incorporated by living cells (2D or 3D) without causing any harm, (2) to act as protein self‐assembly inducers, and (3) to transfer their long‐lasting fluorescence and electroactivity properties to the protein‐fluorophore microfibers. The process is driven by cell's own metabolism and leads to the generation of stable multifunctional microfibers exhibiting hierarchical order across several length‐scales with morphologies varying from helical wires to rigid rods. Analogous microfibers are obtained following uptake of an appropriate thiophene fluorophore by small living organisms (Hydra vulgaris). Due to the high photostability of thiophene fluorophores, the entire process of fibers formation can be tracked in real time by LSCM. Moreover, the microfibers can be picked up and separated by their environment to assess their composition and properties by a variety of techniques including electrophoretic analyses and Q‐TOF mass spectrometry, Tr‐TUNA AFM and KPFM. This review also reports DFT calculations aimed to provide indications for the design of appropriate thiophene fluorophores for microfibers formation inside live cells.
Knowledge, risk assessment, practices,self‐efficacy, attitudes, and behaviour's towards venous thromboembolism among nurses: A systematic review
Authors: Al‐Mugheed Khalid , Bayraktar Nurhan 
Published: June 2023

AbstractAimThis study reviewed the literature on nurses' knowledge, risk assessment practices, self‐efficacy, attitudes, and behaviours towards venous thromboembolism (VTE).DesignA systematic review following PRISMA guidelines.MethodsCINAHL (via EBSCO), MEDLINE (via PubMed), and Web of Science were electronic databases used to find studies published from 2010 to November 2020 in English language. A Hoy critical appraisal checklist was used to assess the risk of bias and methodologic quality.ResultsThis study included fourteen studies conducted on 8628 Registered Nurses. Nine of the fourteen studies examined nurses' general knowledge level regarding VTE, and five showed that most nurses had a good knowledge of VTE. Of the 14 studies, six assessed nurses' risk assessment knowledge regarding VTE, and three showed that nurses had low knowledge of VTE risk assessment. Eleven studies assessed nurses' practices concerning VTE prophylaxis; 5 of the 11 studies reported that nurses had poor and unsatisfactory levels of VTE practice. Three of the 14 studies showed that nurses had low self‐efficacy and varied beliefs. The most frequent recommendations were to create continuous educational programs and in‐service training programs (n = 11), followed by creating institutional protocols standardizing VTE (n = 6).ConclusionsComprehensive educational programs and campaigns based on well‐established and standardized tools should be provided to nurses to improve their VTE knowledge.
Effects of N‐acetylcysteine on oxidative stress biomarkers, depression, and anxiety symptoms in patients with multiple sclerosis
Authors: Khalatbari Mohseni Golsa , Hosseini Seyed Ahmad , Majdinasab Nastaran , Cheraghian Bahman 
Published: June 2023

AbstractAimN‐acetylcysteine (NAC), a thiol‐containing antioxidant and glutathione (GSH) precursor, attenuates oxidative stress, and possibly improves psychiatric disorders. This study aimed to evaluate the effects of oral NAC on oxidative stress, depression, and anxiety symptoms in patients with multiple sclerosis (MS).MethodsThis clinical trial was conducted on 42 MS patients randomly assigned to intervention (n = 21) and control (n = 21) groups. The intervention group received 600 mg of NAC twice daily for 8 weeks, and the control group received a placebo with the same prescription form. An analysis of serum malondialdehyde (MDA), serum nitric oxide (NO), and erythrocyte GSH was carried out on both groups, along with a complete blood count. The Hospital Anxiety and Depression Scale (HADS) was used to assess symptoms of depression (HADS‐D) and anxiety (HADS‐A).ResultsCompared to the control group, NAC consumption significantly decreased serum MDA concentrations (−0.33 [−5.85–2.50] vs. 2.75 [−0.25–5.22] μmol/L; p = 0.03) and HADS‐A scores (−1.6 ± 2.67 vs. 0.33 ± 2.83; p = 0.02). No significant changes were observed in serum NO concentrations, erythrocyte GSH levels, and HADS‐D scores (p > 0.05).ConclusionsBased on the findings of the present study, NAC supplementation for 8 weeks decreased lipid peroxidation and improved anxiety symptoms in MS patients. The aforementioned results suggest that adjunctive therapy with NAC can be considered an effective strategy for MS management. Further randomized controlled studies are warranted.
Evaluation in nonprofit organizations: A defense of perception as a managerial and scientific tool
Authors: Plaisance Guillaume 
Published: June 2023

AbstractNonprofit organizations (NPOs) are subject to multiple pressures from their stakeholders. Stakeholders are at the same time the target of the organization's mission, the evaluators, the providers of resources and the demanders. This article is an essay that defends reciprocal perception as a managerial tool. The proposed intellectual path leads to the revalorization of stakeholder perception as a very useful practical and scientific tool for the management and study of NPOs. However, perception has limitations that the introduction of the concept of reciprocity can address.
Recent trends in thermo‐responsive elastomeric shape memory polymer nanocomposites
Authors: Gopinath Sithara , Adarsh Nayarassery N. , Radhakrishnan Nair P. , Mathew Suresh 
Published: June 2023

AbstractShape memory polymers (SMPs) are polymeric smart materials, a class of stimuli‐responsive polymers that can return to their initial shape from a programmed temporary shape under the application of external stimuli, such as light, heat, magnetism, and electricity. Because of these unique features, SMPs can find applications in many fields, like aerospace, biomedical devices, flexible electronics, soft robotics, shape memory arrays, and 4D printing. The comparatively low density, low cost, easy biodegradability, and biocompatibility make SMPs a better candidate for shape memory applications. Among them, thermo‐responsive SMPs can revert to their permanent shape depending on a temperature greater than their polymer transition temperature. Thermo‐responsive SMPs combine semi‐crystalline or elastomeric polymers with improved mechanical and electrical properties. Integrating nanofillers into the polymer elastomer matrices can further enhance these properties, forming shape‐memory polymer nanocomposites (SMPNCs). This review focuses on the basics, design, and classifications of thermo‐responsive SMPs and the characteristics of elastomers and blends of polymers used. Incorporating various nanofillers to get SMPNCs to have improved properties over SMPs is also presented. The importance of Tg (glass transition temperature) and Tm (melting temperature) based SMPs and SMPNCs, various elastomers used, and the methods for preparation like solution casting, melt compounding, in situ polymerization, and their possible future applications are also discussed.
Leaves micromorphology, chemical profile, and bioactivity of in vitro‐propagated Nepeta cyrenaica (Lamiaceae)
Authors: Alimpić Aradski Ana , Oalđe Pavlović Mariana , Janošević Dušica , Todorović Slađana , Gašić Uroš , Mišić Danijela , Pljevljakušić Dejan , Šavikin Katarina , Marin Petar D. , Giweli Abdulhmid , Duletić‐Laušević Sonja 
Published: June 2023

AbstractIntroductionThe endemic species Nepeta cyrenaica Quézel & Zaffran, native to northeastern Libya, is valued as an important honey‐bearing plant.ObjectivesThis study was aimed to examine the micromorphology, phytochemistry, and bioactivity of in vitro‐propagated N. cyrenaica for the first time.Materials and MethodsThe leaf indumentum was examined using light and scanning electron microscopy and further characterised for histochemistry. The chemical composition of essential oil (EO) was performed using GC‐MS analysis, while dichloromethane (DCM), methanol (ME), ethanol (ET), and aqueous (AQ) extracts were analysed using qualitative and quantitative LC/MS analyses. The antioxidant activities of EO and extracts were assessed using three parallel assays, while enzyme‐inhibiting effects were evaluated against four enzymes.ResultsThe leaves bear various types of glandular trichomes, with lipophilic secretion predominating. The main EO component of EO was 1,8‐cineole. A considerable number of phenolics and iridoids were tentatively identified in the ME extract. Quantitative LC/MS analysis confirmed that ferulic acid, rosmarinic acid, and epigallocatechin gallate were present in the highest amount in the extracts, in which three iridoids were also quantified. Although the ME extract contained the highest amount of polyphenolics and iridoids, the DCM extract showed the best overall biological potential. Additionally, EO exerted the strongest acetylcholinesterase and tyrosinase inhibition.ConclusionThis study demonstrated that the endemic N. cyrenaica can be efficiently grown under in vitro conditions, where it develops various glandular trichomes that are thought to secrete and/or accumulate bioactive compounds with valuable medicinal potential.