bluuebunny/crossref_metadata_2025_split huggingface dataset:
Registry of Ischemic Heart Disease Patients in the Region of Mid Delta, Egypt Data Base and Quality Initiative Project
Authors: Shone Abdelrahman Adel Taha , Elbarbary Mohammed Ahmed , Elhefnawy Suzan Bayoumy , Elsaidy Mona Adel 
Published: September 2023

Background: Cardiovascular diseases are significant contributors to global increased mortality rates. coronary artery disease (CAD) is the most common cause of heart disease globally.
Aims: Detection of the characteristics [clinical, electrocardiographic, echocardiographic and coronary angiographic] of ischemic heart disease patients in Med Delta region in Egypt and also their management plan.
Methodology: The registry included 1000 patients of ischemic heart disease (IDH) at Med Delta Region. All patients underwent full medical history, full cardiac examination, detection of ECG changes, cardiac enzymes, other labs, Echocardiography and coronary angiography
Results: Out of 1000 patients studied 72.1% had chronic coronary syndromes and 27.9% had acute coronary syndrome. Seven hundreds and sixty-seven (76.7%) were known to have hypertension and 725 (72.5%) patients were smokers. left anterior descending artery (LAD) was diseased in 54.9 % of the patients. Single vessel disease was affected in 61.7%, 21.5% had double vessel disease and about 16.8 % had multi vessel disease.
Conclusion: Chronic coronary syndrome was the most common presentation in the studied patients with hypertension being the most prevalent risk factor and LAD was the mostly affected vessel.
Integrated Management of Blast Disease in Finger Millet
Authors: Anand T. , Senthilraja G. , Senthilkumar P. 
Published: September 2023

Aims: To test the effect of bacterial biocontrol agents (Pseudomonas-TNAU-Pf1) and fungicides (Tricyclazole) individually and their integration as seed treatment and foliar spray against finger millet blast under field conditions.
Study Design: The field experiments were conducted in a Randomized Block design.
Place and Duration of Study: The experiments were conducted at the Regional Research Station farm, Tamil Nadu Agricultural University, Paiyur, Krishnagiri District, Tamil Nadu, India, during 2016-17 and 2017-18.
Methodology: Two field experiments were conducted to evaluate the efficacy of talc-based formulation of Pseudomonas -TNAU-Pf1 and Tricyclazole individually and in combination as seed treatment and foliar application for the management of finger millet blast with five treatments and four replications using a variety Paiyur 2. The bioagent and the fungicide were applied as seed treatment and foliar spray.
Results: The results revealed that seed treatment with talc-based formulation of Pseudomonas -TNAU-Pf1 (10g/kg) plus two sprays of tricyclazole (0.1%) one at the maximum tillering phase and another at the heading phase was significantly most effective in reducing leaf, neck and finger blast in both the seasons. The treatment recorded 1.65 and 1.25PDI of leaf blast, 0.33 and 0.22% of neck and 2.55 and 1.33% of finger blast in the first and second season, respectively. The treatment also recorded highest grain yield of 2663 and 2543 kg/ha with BC ratio of 1:3.94 and 1:3.85 in the first and second season, respectively.
Conclusion: Seed treatment with Pseudomonas TNAU-Pf1 (10g/kg) plus two sprays of tricyclazole (0.1%) was found to be effective in reducing the incidence of blast disease and increasing grain yield in finger millet.
Identification of Contrasting Parents and Development of Mapping Population for Sucking Pest Resistance in Cotton
Authors: Akash A. , Boopathi N. Manikanda , Ravikesavan R. , Subramanian A. , Thirukumaran K. 
Published: September 2023

The current study was conducted to identify the best parents with good agronomical features and contrasting characteristics for sucking pest resistance to develop a population that can be used for Quantitative Trait Loci mapping of resistance component traits. Initial attempts at this university have enabled to identify of five potential parents (viz., KC3, NDLH 1938, CO18, K12, and RG8) with improved sucking pest resistance and yield characters and they were crossed with the recently released high-yielding variety CO-17, which is also suitable for high-density planting. All these six parents were evaluated for fiber yield and quality traits besides anatomical characters such as trichomes density, length, width, and sharpness that confers sucking pest resistance and genotyped with 200 Simple Sequence Repeats markers that span the entire genome. Efforts have been dedicated to the careful selection of optimal parents, namely CO17 and KC3, each possessing distinct traits related to resistance against sucking pests and also fiber quality characters in addition. This strategic approach aims to combine their contrasting genetic components for sucking pest resistance, to develop a new generation of plants that exhibit heightened resilience to these types of pests. CIR139, a polymorphic SSR marker of these two parents was used to fix the true hybrids which were advanced to generate an F2 mapping population. This population is believed to be useful for QTL mapping of anatomical features that confer sucking pest resistance such as trichome length, density, breadth, and sharpness besides fiber yield and quality traits.
Study of Ground Water Behaviour in the Tons Pump Canal Command Area of Karchhana Tehsil, Prayagaraj
Authors: Singh Sachchida Nand , Alam Mohd. Aftab 
Published: September 2023

The study of groundwater behaviour in the Tons Pump canal command area of Karchhana Tehsil was carried out by utilizing ground water table data from 1997 to 2021 (25 years). During the study period, the pre-monsoon water table depth ranged from 3.25 m to 19.55 m, whereas the post-monsoon depth ranged from 1.71 m to 17.70 m. The water table trend in the study area during the pre-monsoon season revealed that at 83.55% of the locations having falling trend, while the remaining 16.45% experienced neither rising nor falling trend in the water table. During post-monsoon season, the water table was falling at 89.99% locations, with the rest 10.10% having neither rising nor falling trend. Therefore, the study found that the majority of the study area was experiencing water table fall due to over-exploitation of ground water in the both pre and post-monsoon season. Development stages of the groundwater utilization study from 1997 to 2021 showed that during the year 1997, all block of the study area was found under safe category.The overall utilisation of groundwater development stage was determined to be 44.93%. In 2021, the overall development stage of groundwater utilization was found to be 64.11% and the entire study area comes under safe category of groundwater utilization. It was found that groundwater levels in the study area were progressively rising. Therefore, it was necessary to enhance the surface water supply through canal systems to reduce the draft of groundwater as well as artificial groundwater recharge is necessary to arrest the groundwater at the desired level in the study area.
Impact of Diameter of Inhibition in Crowded Plates on the in vitro Inhibitory Strength of Potential Antibiotic-Producing Bacteria Isolated from Some Natural Habitats
Authors: Balogun Olasinbo Olumuyiwa , Ugoh Sylvanus Chukwudi , Abioye Olabisi Peter , Oladosu Peters Oluwale 
Published: September 2023

Antimicrobial resistance is a global problem, and efforts to replace failing antibiotics are crucial. We investigated the impact of the diameter of inhibition (mm) in crowded plates of potential antibiotic-producing bacteria on their in-vitro inhibitory strength. Our previous research identified 12 potential antibiotic-producing bacteria from natural habitats and obtained their diameters of inhibition in preliminary crowded plates. The potential candidates were further challenged with some pathogenic microbes by the Agar-well diffusion technique to test their strength. The results showed that the diameter of inhibition of the potential antibiotic-producing isolates obtained at the crowded plate technique level was not a predictor of the potency of potential antibiotic-producing bacteria (rs = 0.52, P = 0.08). In summary, the selection of potent candidate microbes for the development of new antimicrobial compounds against multidrug-resistant pathogens cannot be determined based on the diameter of inhibition in a preliminary investigation using the crowded plate technique. Further studies involving complementary techniques are necessary to determine the best potential candidate organisms for the development of novel antimicrobial compounds.
Overall Survival Among Patients With De Novo Stage IV Metastatic and Distant Metastatic Recurrent Non–Small Cell Lung Cancer
Authors: Su Chloe C. , Wu Julie T. , Choi Eunji , Myall Nathaniel J. , Neal Joel W. , Kurian Allison W. , Stehr Henning , Wood Douglas , Henry Solomon M. , Backhus Leah M. , Leung Ann N. , Wakelee Heather A. , Han Summer S. 
Published: September 2023

ImportanceDespite recent breakthroughs in therapy, advanced lung cancer still poses a therapeutic challenge. The survival profile of patients with metastatic lung cancer remains poorly understood by metastatic disease type (ie, de novo stage IV vs distant recurrence).ObjectiveTo evaluate the association of metastatic disease type on overall survival (OS) among patients with non–small cell lung cancer (NSCLC) and to identify potential mechanisms underlying any survival difference.Design, Setting, and ParticipantsCohort study of a national US population based at a tertiary referral center in the San Francisco Bay Area using participant data from the National Lung Screening Trial (NLST) who were enrolled between 2002 and 2004 and followed up for up to 7 years as the primary cohort and patient data from Stanford Healthcare (SHC) for diagnoses between 2009 and 2019 and followed up for up to 13 years as the validation cohort. Participants from NLST with de novo metastatic or distant recurrent NSCLC diagnoses were included. Data were analyzed from January 2021 to March 2023.ExposuresDe novo stage IV vs distant recurrent metastatic disease.Main Outcomes and MeasuresOS after diagnosis of metastatic disease.ResultsThe NLST and SHC cohort consisted of 660 and 180 participants, respectively (411 men [62.3%] vs 109 men [60.6%], 602 White participants [91.2%] vs 111 White participants [61.7%], and mean [SD] age of 66.8 [5.5] vs 71.4 [7.9] years at metastasis, respectively). Patients with distant recurrence showed significantly better OS than patients with de novo metastasis (adjusted hazard ratio [aHR], 0.72; 95% CI, 0.60-0.87; P &amp;lt; .001) in NLST, which was replicated in SHC (aHR, 0.64; 95% CI, 0.43-0.96; P = .03). In SHC, patients with de novo metastasis more frequently progressed to the bone (63 patients with de novo metastasis [52.5%] vs 19 patients with distant recurrence [31.7%]) or pleura (40 patients with de novo metastasis [33.3%] vs 8 patients with distant recurrence [13.3%]) than patients with distant recurrence and were primarily detected through symptoms (102 patients [85.0%]) as compared with posttreatment surveillance (47 patients [78.3%]) in the latter. The main finding remained consistent after further adjusting for metastasis sites and detection methods.Conclusions and RelevanceIn this cohort study, patients with distant recurrent NSCLC had significantly better OS than those with de novo disease, and the latter group was associated with characteristics that may affect overall survival. This finding can help inform future clinical trial designs to ensure a balance for baseline patient characteristics.
Deep Learning Empowers the Discovery of Self‐Assembling Peptides with Over 10 Trillion Sequences
Authors: Wang Jiaqi , Liu Zihan , Zhao Shuang , Xu Tengyan , Wang Huaimin , Li Stan Z. , Li Wenbin 
Published: September 2023

AbstractSelf‐assembling of peptides is essential for a variety of biological and medical applications. However, it is challenging to investigate the self‐assembling properties of peptides within the complete sequence space due to the enormous sequence quantities. Here, it is demonstrated that a transformer‐based deep learning model is effective in predicting the aggregation propensity (AP) of peptide systems, even for decapeptide and mixed‐pentapeptide systems with over 10 trillion sequence quantities. Based on the predicted AP values, not only the aggregation laws for designing self‐assembling peptides are derived, but the transferability relation among the APs of pentapeptides, decapeptides, and mixed pentapeptides is also revealed, leading to discoveries of self‐assembling peptides by concatenating or mixing, as consolidated by experiments. This deep learning approach enables speedy, accurate, and thorough search and design of self‐assembling peptides within the complete sequence space of oligopeptides, advancing peptide science by inspiring new biological and medical applications.
Improved Humoral Immunity and Protection against Influenza Virus Infection with a 3d Porous Biomaterial Vaccine
Authors: Miwa Hiromi , Antao Olivia Q. , Kelly‐Scumpia Kindra M. , Baghdasarian Sevana , Mayer Daniel P. , Shang Lily , Sanchez Gina M. , Archang Maani M. , Scumpia Philip O. , Weinstein Jason S , Di Carlo Dino 
Published: September 2023

AbstractNew vaccine platforms that activate humoral immunity and generate neutralizing antibodies are required to combat emerging pathogens, including influenza virus. A slurry of antigen‐loaded hydrogel microparticles that anneal to form a porous scaffold with high surface area for antigen uptake by infiltrating immune cells as the biomaterial degrades is demonstrated to enhance humoral immunity. Antigen‐loaded‐microgels elicited a robust cellular humoral immune response, with increased CD4+ T follicular helper (Tfh) cells and prolonged germinal center (GC) B cells comparable to the commonly used adjuvant, aluminum hydroxide (Alum). Increasing the weight fraction of polymer material led to increased material stiffness and antigen‐specific antibody titers superior to Alum. Vaccinating mice with inactivated influenza virus loaded into this more highly cross‐linked formulation elicited a strong antibody response and provided protection against a high dose viral challenge. By tuning physical and chemical properties, adjuvanticity can be enhanced leading to humoral immunity and protection against a pathogen, leveraging two different types of antigenic material: individual protein antigen and inactivated virus. The flexibility of the platform may enable design of new vaccines to enhance innate and adaptive immune cell programming to generate and tune high affinity antibodies, a promising approach to generate long‐lasting immunity.
In Vivo Intra‐Uterine Delivery of TAT‐Fused Cre Recombinase and CRISPR/Cas9 Editing System in Mice Unveil Histopathology of Pten/p53‐Deficient Endometrial Cancers
Authors: Navaridas Raúl , Vidal‐Sabanés Maria , Ruiz‐Mitjana Anna , Altés Gisela , Perramon‐Güell Aida , Yeramian Andree , Egea Joaquim , Encinas Mario , Gatius Sonia , Matias‐Guiu Xavier , Dolcet Xavier 
Published: September 2023

AbstractPhosphatase and TENsin homolog (Pten) and p53 are two of the most frequently mutated tumor suppressor genes in endometrial cancer. However, the functional consequences and histopathological manifestation of concomitant p53 and Pten loss of function alterations in the development of endometrial cancer is still controversial. Here, it is demonstrated that simultaneous Pten and p53 deletion is sufficient to cause epithelial to mesenchymal transition phenotype in endometrial organoids. By a novel intravaginal delivery method using HIV1 trans‐activator of transcription cell penetrating peptide fused with a Cre recombinase protein (TAT‐Cre), local ablation of both p53 and Pten is achieved specifically in the uterus. These mice developed high‐grade endometrial carcinomas and a high percentage of uterine carcinosarcomas resembling those found in humans. To further demonstrate that carcinosarcomas arise from epithelium, double Pten/p53 deficient epithelial cells are mixed with wild type stromal and myometrial cells and subcutaneously transplanted to Scid mice. All xenotransplants resulted in the development of uterine carcinosarcomas displaying high nuclear pleomorphism and metastatic potential. Accordingly, in vivo CRISPR/Cas9 disruption of Pten and p53 also triggered the development of metastatic carcinosarcomas. The results unfadingly demonstrate that simultaneous deletion of p53 and Pten in endometrial epithelial cells is enough to trigger epithelial to mesenchymal transition that is consistently translated to the formation of uterine carcinosarcomas in vivo.
Optically Programmable Living Microrouter in Vivo
Authors: Liu Xiaoshuai , Wu Huaying , Wu Shuai , Qin Haifeng , Zhang Tiange , Lin Yufeng , Zheng Xianchuang , Li Baojun 
Published: September 2023

AbstractWith high reconfigurability and swarming intelligence, programmable medical micromachines (PMMs) represent a revolution in microrobots for executing complex coordinated tasks, especially for dynamic routing of various targets along their respective routes. However, it is difficult to achieve a biocompatible implantation into the body due to their exogenous building blocks. Herein, a living microrouter based on an organic integration of endogenous red blood cells (RBCs), programmable scanning optical tweezers and flexible optofluidic strategy is reported. By harvesting energy from a designed optical force landscape, five RBCs are optically rotated in a controlled velocity and direction, under which, a specific actuation flow is achieved to exert the well‐defined hydrodynamic forces on various biological targets, thus enabling a selective routing by integrating three successive functions, i.e., dynamic input, inner processing, and controlled output. Benefited from the optofluidic manipulation, various blood cells, such as the platelets and white blood cells, are transported toward the damaged vessel and cell debris for the dynamic hemostasis and targeted clearance, respectively. Moreover, the microrouter enables a precise transport of nanodrugs for active and targeted delivery in a large quantity. The proposed RBC microrouter might provide a biocompatible medical platform for cell separation, drug delivery, and immunotherapy.
Occupational injuries and illnesses among law enforcement officers, 2001−2019: Findings from the Ohio Bureau of Workers' Compensation
Authors: Tiesman Hope M. , Konda Srinivas , Wurzelbacher Steven J. , Naber Steven J. , Attwood Wesley R. 
Published: September 2023

AbstractBackgroundOccupational injuries are common among law enforcement officers (LEOs) and can impact an agency's ability to serve communities. Workers' compensation (WC) data are an underutilized source for occupational injury surveillance in the law enforcement field.MethodsLEOs WC claims from the Ohio Bureau of Workers' Compensation (OHBWC) from 2001 to 2019 were identified based on manual review of the occupation title and injury description. Worker, employer, incident, and injury characteristics were described by claim type—medical‐only (MO) and lost‐time (8 or more days away from work). Data are presented using injury claim counts.ResultsFrom 2001 to 2019, 50,793 WC claims were identified among Ohio LEOs. Of these, 68% were MO claims (n = 34,622). WC claims significantly decreased over the 19‐year period (p < 0.001). Seventy‐five percent of WC claims were from a LEO with more than one claim and of these, 34% were from a LEO with five or more claims during the study period. Male officers and those aged 25−54 years incurred the highest proportion of total claims (87.8% & 91.8%, respectively). Violence (n = 17,247; 34%), falls/slips/trips (n = 9079; 17.9%), and transportation events (n = 7977; 15.7%) were the leading events. Among the 50,793 claims, there were 79,637 unique clinical diagnosis groups. The most common injury diagnoses were sprains (n = 32,796; 41.2%) followed by contusions (n = 13,529; 17%).ConclusionsResults can guide the development or improvement of workplace injury prevention strategies for LEOs. Efforts should be focused on better understanding and preventing violent injury events and sprains among LEOs, as well as preventing multiple injury events.
Ultrafluorogenic Monochromophore‐Type BODIPY‐Tetrazine Series for Dual‐Color Bioorthogonal Imaging with a Single Probe
Authors: Kim Dahham , Son Hayoung , Park Seung Bum 
Published: September 2023

AbstractVarious fluorogenic probes utilizing tetrazine (Tz) as a fluorescence quencher and bioorthogonal reaction partner have been extensively studied over the past few decades. Herein, we synthesized a series of boron‐dipyrromethene (BODIPY)‐Tz probes using monochromophoric design strategy for bioorthogonal cellular imaging. The BODIPY‐Tz probes exhibited excellent bicyclo[6.1.0]nonyne (BCN)‐selective fluorogenicity with three‐ to four‐digit‐fold enhancements in fluorescence over a wide range of emission wavelengths, including the far‐red region. Furthermore, we demonstrated the applicability of BODIPY‐Tz probes in bioorthogonal fluorescence imaging of cellular organelles without washing steps. We also elucidated the aromatized pyridazine moiety as the origin of BCN‐selective fluorogenic behavior. Additionally, we discovered that the fluorescence of the trans‐cyclooctene (TCO) adducts was quenched in aqueous media via photoinduced electron transfer (PeT) process. Interestingly, we observed a distinctive recovery of the initially quenched fluorescence of BODIPY‐Tz‐TCO upon exposure to hydrophobic media, accompanied by a significant bathochromic shift of its emission wavelength relative to that exhibited by the corresponding BODIPY‐Tz‐BCN. Leveraging this finding, for the first time, we achieved dual‐color bioorthogonal cellular imaging with a single BODIPY‐Tz probe.
External genitalia phenotypes of a Mab21l1‐null mouse model for cerebellar, ocular, craniofacial, and genital (COFG) syndrome
Authors: Promsut Watcharapon , Yamada Ryuichi , Takami Shohei , Miyazaki Nanae , Uemura Mami , Hiramatsu Ryuji , Takahashi Naoki , Kanai Yoshiakira 
Published: September 2023

AbstractThe cerebellar, ocular, craniofacial, and genital (COFG) syndrome is a human genetic disease that is caused by MAB21L1 mutations. A COFG mouse model with Mab21l1‐null mutation causes severe microphthalmia and fontanelle dysosteogenesis, similar to the symptoms in human patients. One of the typical symptoms is scrotal agenesis in male infants, while male Mab21l1‐null mice show hypoplastic preputial glands, a rodent‐specific derivative of the cranial scrotal fold. However, it is still unclear where and how MAB21Ll acts in the external genitalia in both mice and humans. Here we show that, at the neonatal stage, MAB21L1 expression in the external genitalia was restricted to two mesenchymal cell populations—underneath the scrotal and labial skin and around the preputial and clitoral glands (PG/CG). Morphometric analyses of the Mab21l1−/− pups revealed a significant reduction in the external size of the scrotum, vulva, and CG, as well as PG. In the periglandular region around PG and CG, the periglandular mesenchymal cells showed a drastic reduction in both cell density and immunoreactive signals for several extracellular matrix proteins (e.g., collagen I, fibronectin, and proteoglycans), together with their reduced Ki67‐positive cell proliferation index. In the Mab21l1−/− PG/CG, together with reduced vascularization, the glandular epithelia displayed atrophy with discontinuous basal lamina along the basal surface and defective glycogen accumulation in their cytoplasm. Under a 5‐day organ culture of the isolated PG, the Mab21l1−/− explants showed poor outgrowth and retention of the glandular structure in vitro. However, the addition of exogenous Matrigel could partially rescue such tissue‐autonomous phenotypes, showing glandular morphology similar to that of the wild‐type explants. These findings suggest that MAB21L1+ mesenchymal cells play a crucial role in providing nutrient ECM support for glandular outgrowth and morphogenesis in the peripheral external genitalia.