bluuebunny/crossref_metadata_2025_split huggingface dataset:
Pengembangan Rancangan Pembelajaran Berbasis Aplikasi Tahu Terguri Bagi Guru PAUD
Authors: Kartini Kartini , Suyatmin Suyatmin 
Published: December 2022

Latar belakang penelitian adalah guru mengalami kesulitan dalam sistematika rancangan pembelajaran, meliputi: Program Tahunan, Program Semester, Rancangan Pelaksanaan Pembelajaran Mingguan dan Rancangan Pelaksanaan Pembelajaran Harian. Tujuannya adalah menghasilkan aplikasi yang dapat membantu guru dalam merancang perangkat pembelajaran. Jenis penelitian menggunakan penelitian pengembangan terdiri dari sepuluh tahap: Studi pendahuluan, perencanaan penelitian, pengembangan produk awal, uji lapangan terbatas, revisi uji lapangan terbatas, uji lapangan lebih luas, revisi hasil uji lapangan, uji kelayakan, revisi hasil uji kelayakan, diseminasi dan sosialaisasi produk akhir. Uji coba produk skala kecil dilakukan di tiga PAUD dengan jumlah responden sebanyak 6 guru sedangkan pada uji coba skala besar dilakukan di lima PAUD dengan jumlah responden sebanyak 10 guru. Teknik pengumpulan data penelitian dilakukan melalui wawancara dan angket. Teknik Analisis data penelitian yakni analisis desktiptif kuantitatif dan analisis deskriptif kualitatif. Hasil penelitian menunjukkan bahwa pengembangan rancangan pembelajaran berbasis aplikasi Tahu Terguri layak digunakan dan dapat diproduksi massal.
Salud ambiental: una necesidad educativa para los médicos del Perú
Authors: Ccami-Bernal Fabricio , Barriga-Chambi Fernanda , Fernandez-Guzman Daniel , Vergara-de la Rosa Esteban , Galvez-Olortegui Jose 
Published: December 2022

Introducción: La implementación de cursos relacionados a la salud ambiental en las currículas de pregrado favorece la formación de médicos más comprometidos con el impacto del cambio climático sobre la salud. El objetivo del estudio fue identificar la prevalencia de cursos lectivos sobre salud ambiental en facultades de medicina del Perú, así como su comparación con los niveles de calidad de aire por cada región del país.
Métodos: Se realizó un estudio descriptivo. Se buscó en la página web de la Superintendencia Nacional de Educación Superior Universitaria (SUNEDU) del Perú y se seleccionó aquellas universidades con facultad de medicina humana registradas. Se analizó si estas incluían en su currículo un curso relacionado a medicina ambiental y se comparó con los niveles de calidad de aire (Material particulado) por cada región.
Resultados: De 41 facultades de medicina incluidas, 26 (63,4%) de ellas incluyeron en su currículum un curso relacionado a salud ambiental, y 2 (7,7%) de ellas lo incluyeron como un curso opcional. De las regiones con un índice de calidad de aire moderado/malo, el 63% cuentan con facultades de medicina con cursos lectivos sobre medicina ambiental.
Conclusiones: Es necesario estandarizar su metodología, contenidos y recursos de enseñanza, así como el estudio de temas relevantes como el cambio climático, además de su integración con los demás cursos de la carrera médica dada la importancia que cobra la salud ambiental en las diferentes regiones del Perú.
Periodontitis and Diabetes: How Well the Patients have Knowledge about the Established Interrelationship?
Authors: Ranjit R. , Bista S. , Manandhar P. , Subedi S. 
Published: December 2022

Background Type 2 diabetes is an escalating health problem in Nepal and it holds a strong bidirectional Inter relationship with periodontitis. However, lack of its knowledge and motivation among patients may deteriorate and complicate their condition.
Objective To assess knowledge among general population regarding the relation between diabetes and periodontitis and to evaluate the role of dentists and physicians in suggesting the diabetic patients for oral health care.
Method A cross-sectional study was conducted among the patients visiting Department of Periodontics, Gandaki Medical College. A total 422 patients were interviewed using a set of pretested questionnaire and at the end they were counselled about the impact of diabetes mellitus on periodontal status and vice versa.
Result Majority of the patients 257 (60.9%) had no idea about the interrelation between periodontitis and diabetes mellitus. Almost all the patients 363 (86.02%) assumed that poor oral health cannot increase the risk of developing diabetes and amongst those who had knowledge about this relation, the major source of information was their diabetic friends and relatives 46 (10.9%) and other sources such as syllabus 46 (10.9%). Only handful of patients 30 (7.10%) were informed about the relation by their dentist. Ironically, none of them were provided information about the interrelation by their treating physician.
Conclusion Overall, only few participants had knowledge about the bidirectional relation between periodontitis and diabetes. Hence, our findings support the greater need for more targeted and specific health education along with close collaboration between dentists and physicians.
Inisiasi Gerakan Penyemprotan Hama Padi dan Jagung secara Massal di Desa Soropaten, Kecamatan Karanganom, Kabupaten Klaten
Authors: Huwaida Salma Nabila , Huwaida Salma Nabila , Handayani Galuh Setya , Hamdah Luthfiah Nurul , Aditya Yoga Pratama , Saputra Alifyan Arza , Amelia Ellora , Putri Ardelia Zahrani , Ardiansyah Raihan , Fadhilah HS Farah , Laksmana Bella Aprila Azzahra 
Published: December 2022

Desa Soropaten merupakan salah satu desa yang terletak di Kecamatan Karanganom, Kabupaten Klaten, Provinsi Jawa Tengah. Data BPS menunjukkan luas lahan sawah setempat per 2021 seluas 121,57 (Ha) dari luas seluruh wilayah seluas 166,44 (Ha) yang didominasi oleh tanaman pangan berupa jagung dan padi. Kendala yang dialami oleh petani setempat adalah adanya penurunan produktivitas karena serangan hama. Hama wereng pada padi dan hama ulat grayak pada jagung menjadi hama yang merugikan petani. Kegiatan pengabdian masyarakat ini menginisiasi gerakan penyemprotan hama padi dan jagung secara massal bersama Gapoktan. Kegiatan pengabdian ini dilakukan untuk menambah pengetahuan petani dalam mengendalikan hama pada tanaman budidaya dalam gerakan penyemprotan massal. Gerakan penyemprotan dapat terlaksana melalui pembekalan pengetahuan melalui tahapan kegiatan yang meliputi (1) perencanaan program; 2) sosialisasi program; 3) penyuluhan pertanian; 4) penyemprotan hama jagung; dan 5) penyemprotan hama padi. Melalui kegiatan ini, petani diharapkan senantiasa melakukan kegiatan serupa secara rutin sebagai upaya pengendalian hama.
MİKROPLARIN VE ZEHİRLERİN SAVAŞ MEYDANLARINDA KULLANILMASI: ORTAÇAĞ’DA BİYOLOJİK SAVAŞ
Authors: KARAİMAMOĞLU Tolgahan 
Published: December 2022

ÖZ
İnsanlık tarihin her döneminde birçok savaş meydana gelmiştir. İrili-ufaklı ordular arasındaki bu savaşlarda ise sadece klasik silahlar kullanılmamıştır. Gündelik hayat içerisinde insan sağlığına zararlı ve genellikle de ölümcül alan ajanlarda kimi zaman savaş meydanlarında etkili birer silah haline dönüştürülmüştür. Dolayısıyla biyolojik savaşın tarihi çok eskilere dayanmakta ve hiç bitmeden de varlığını, insanlık için tehdit olma durumunu sürdürmektedir. Biyolojik unsurların ve zehirlerin kullanımına bakıldığında ise ortaçağ dünyasında dikkat çekici örnekler bulmak mümkündür. Artan nüfus nispetinde daha büyük ordularla savaşların yapılmaya başlaması ortaçağ süresince zehrin ve biyolojik unsurların harp sahalarında giderek daha yoğun ve sofistike şekilde kullanımını ortaya çıkartmıştır. Ortaçağda surlarla kaplı şehirlerin kuşatılması esnasında hastalıktan ölmüş ve hastalık saçan insan ve hayvan ölülerinin mancınıklarla attırılarak düşman kuvvetlerinin hastalıktan kırılmasının amaçlanması en belirgin biyolojik harp tekniği olmuştur. Kara Ölüm ortaçağ dünyasında biyolojik savaşın en açık ve yıkıcı örneği olmuştur. Aynı zamanda gübre ve insan sağlığına zararlı diğer ajanlarda kimi zaman mancınıklarla kalelere attırılmıştır. Hayvanlardan elde edilen veya bilinen zehirler ise özellikle su kuyularını tahrip etmek için kullanılmıştır. Avrupa periferinde ortaçağ süresinde Fransa, İtalya, İspanya ve İngiltere biyolojik unsurların savaş alanında kullanan başlıca ülkeler olmuştur. Özellikle ortaçağın son döneminde İtalyanların biyolojik savaş silahlarını kontrolsüz şekilde geliştirdikleri anlaşılmaktadır. Dolayısıyla bu kontrol ve yıkıcı etkiler ortaya çıkaran biyolojik savaşa karşı Fransa ve Almanya arasında erken devirde üstü kapalı bir anlaşma dahi yapılmıştır. Genel anlamıyla bu araştırma ortaçağ dünyasında bazı örnekler üzerinden zehir ve biyolojik unsurların savaş meydanlarında kullanılmasına odaklanmaktadır.
Bortezomib abrogates temozolomide-induced autophagic flux through an ATG5 dependent pathway
Authors: Rahman Mohummad Aminur , Engelsen Agnete S. T. , Sarowar Shahin , Bindesbøll Christian , Birkeland Even , Goplen Dorota , Lotsberg Maria L. , Knappskog Stian , Simonsen Anne , Chekenya Martha 
Published: December 2022

Introduction: Glioblastoma (GBM) is invariably resistant to temozolomide (TMZ) chemotherapy. Inhibiting the proteasomal pathway is an emerging strategy to accumulate damaged proteins and inhibit their lysosomal degradation. We hypothesized that pre-treatment of glioblastoma with bortezomib (BTZ) might sensitize glioblastoma to temozolomide by abolishing autophagy survival signals to augment DNA damage and apoptosis.Methods: P3 patient-derived glioblastoma cells, as well as the tumour cell lines U87, HF66, A172, and T98G were investigated for clonogenic survival after single or combined treatment with temozolomide and bortezomib in vitro. We investigated the requirement of functional autophagy machinery by utilizing pharmacological inhibitors or CRISPR-Cas9 knockout (KO) of autophagy-related genes -5 and -7 (ATG5 and ATG7) in glioblastoma cells and monitored changes in autophagic flux after temozolomide and/or bortezomib treatments. P3 wild-type and P3 ATG5−/− (ATG5 KO) cells were implanted orthotopically into NOD-SCID mice to assess the efficacy of bortezomib and temozolomide combination therapy with and without functional autophagy machinery.Results: The chemo-resistant glioblastoma cells increased autophagic flux during temozolomide treatment as indicated by increased degradation of long-lived proteins, diminished expression of autophagy markers LC3A/B-II and p62 (SQSTM1), increased co-localisation of LC3A/B-II with STX17, augmented and no induction of apoptosis. In contrast, bortezomib treatment abrogated autophagic flux indicated by the accumulation of LC3A/B-II and p62 (SQSTM1) positive autophagosomes that did not fuse with lysosomes and thus reduced the degradation of long-lived proteins. Bortezomib synergistically enhanced temozolomide efficacy by attenuating cell proliferation, increased DNA double-strand breaks, and apoptosis in an autophagy-dependent manner. Abolishing autophagy in ATG5 KOs reversed the bortezomib-induced toxicity, rescued glioblastoma cell death and reduced animal survival.Discussion: We conclude that bortezomib abrogates temozolomide induced autophagy flux through an ATG5 dependent pathway.
Adeno-associated virus vector intraperitoneal injection induces colonic mucosa and submucosa transduction and alters the diversity and composition of the faecal microbiota in rats
Authors: Ma Li-Tian , Lian Jing-Xuan , Bai Yang , Shang Meng-Juan , Zhang Zhe-Zhe , Wu Fei-Fei , Chen Jing , Meng Xian-Bo , Zheng Jin , Li Tian , Li Yun-Qing , Wang Jing-Jie 
Published: December 2022

BackgroundViral vector technology, especially recombinant adeno-associated virus vector (rAAV) technology, has shown great promise in preclinical research for clinical applications. Several studies have confirmed that rAAV can successfully transduce the enteric nervous system (ENS), and rAAV gene therapy has been approved by the Food and Drug Administration (FDA) for the treatment of the early childhood blindness disease Leber congenital amaurosis and spinal muscular atrophy (SMA). However, until now, it has not been possible to determine the effect of AAV9 on intestinal microbiota. MethodsWe examined the efficiency of AAV9-mediated ascending colon, transverse colon and descending colon transduction through intraperitoneal (IP) injection, performed 16S rRNA gene amplicon sequencing and analysed specific faecal microbial signatures following AAV9 IP injection via bioinformatics methods in Sprague–Dawley (SD) rats. ResultsOur results showed (1) efficient transduction of the mucosa and submucosa of the ascending, transverse, and descending colon following AAV9 IP injection; (2) a decreased alpha diversity and an altered overall microbial composition following AAV9 IP injection; (3) significant enrichments in a total of 5 phyla, 10 classes, 13 orders, 15 families, 29 genera, and 230 OTUs following AAV9 IP injection; and (4) AAV9 can significantly upregulate the relative abundance of anaerobic microbiota which is one of the seven high-level phenotypes that BugBase could predict.ConclusionIn summary, these data show that IP injection of AAV9 can successfully induce the transduction of the colonic mucosa and submucosa and alter the diversity and composition of the faecal microbiota in rats.
Feasibility, effectiveness, and safety of edoxaban administration through percutaneous endoscopic gastrostomy: 12-months follow up of the ORIGAMI study
Authors: Cappannoli Luigi , Laborante Renzo , Galli Mattia , Canonico Francesco , Ciliberti Giuseppe , Restivo Attilio , Princi Giuseppe , Arcudi Alessandra , Sabatelli Mario , De Cristofaro Raimondo , Crea Filippo , D’Amario Domenico 
Published: December 2022

Background and aimsEdoxaban proved to be safe and effective also in fragile patients, but its administration through percutaneous endoscopic gastrostomy (PEG) has not been previously investigated. The purpose of this study was to evaluate the feasibility and the preliminary safety and efficacy profiles of edoxaban administered via PEG in patients with an indication for long-term oral anticoagulation.MethodsORIGAMI was a prospective, single-arm, observational study (NCT04271293). Patients with PEG and an indication for long-term anticoagulation were prospectively enrolled. Crushed edoxaban at approved doses was administered via PEG. The primary endpoint was the composite of cardio-embolic events consisting of ischemic stroke, systemic embolism, or symptomatic deep venous thrombosis/pulmonary embolism (DVT/PE). Secondary endpoints were the number of bleeding events and edoxaban plasma concentrations at steady state. We here report the 12-month results.ResultsA total of 12 patients were enrolled. The main indication for PEG implantation was amyotrophic lateral sclerosis (10/12). The primary endpoint of cardio-embolic events did not occur in any patients at 12 months. All patients were in the therapeutic range of steady-state edoxaban plasma levels. Three minor bleedings were observed, while no major bleedings occurred during the observational period. A total of five patients died. All deaths were from non-cardiovascular causes and were consistent with the natural history of the pre-existing severe disease.ConclusionOur study suggests that edoxaban administration via PEG is feasible and appears safe and effective in fragile, comorbid patients, resulting in therapeutic plasma concentrations of edoxaban.Clinical trial registration[ClinicalTrials.gov], identifier [NCT04271293].
Early detection of immune checkpoint inhibitor-related subclinical cardiotoxicity: A pilot study by using speckle tracking imaging and three-dimensional echocardiography
Authors: Xu Aiqing , Yuan Ming , Zhan Xiaoping , Zhao Gangjian , Mu Guanyu , Wang Tingting , Hu Hailong , Fu Huaying 
Published: December 2022

BackgroundEarly detection of subclinical cardiotoxicity of immune checkpoint inhibitor (ICI) therapy can be challenging.ObjectiveTo evaluate subclinical cardiac dysfunction using two-dimensional speckle tracking imaging (2D-STI) and three-dimensional echocardiography in Chinese patients.MethodsFifty-five consecutive patients with malignant tumors treated by immunotherapy were included. They were examined by echocardiography before immunotherapy and after immunotherapy. Left ventricular ejection fraction (LVEF) was calculated in three-dimensional imaging. Moreover, left ventricular global longitudinal peak systolic strain (LVGLS), left ventricular global circumferential peak systolic strain (LVGCS), right ventricular global longitudinal systolic strain (RVGLS), right ventricular free wall longitudinal peak systolic strain (RVFWLS), and tricuspid annular plane systolic excursion (TAPSE) were evaluated. Clinical and laboratory parameters were recorded. Cardiac toxicity events were defined as the presence of heart failure symptoms, LVEF reduction, and increase in troponin. Subclinical cardiac toxicity was defined as cardiac dysfunction associated with ICI treatment, with absent or delayed ICI-associated cardiotoxicity clinical symptoms.ResultsCompared with baseline, the LVGLS, TAPSE, and RVGLS significantly deteriorated after ICI treatment [(–18.63 ± 2.53)% vs. (–17.35 ± 2.58)%, P = 0.000; 18.29 ± 6.23 vs. 14.57 ± 3.81, P = 0.0001; and (–18.45 ± 4.65)% vs. (–14.98 ± 3.85)%, P = 0.0001, respectively]. LVGLS (–17.35 ± 2.58, P = 0.000), TAPSE (14.57 ± 3.81, P = 0.0001), and RVGLS [(–14.98 ± 3.85)%, P = 0.0001] were decreased after ICI immunotherapy. Kaplan-Meier curve analysis showed that LVGLS was more sensitive than the cardiac toxicity events to assess ICI-related subclinical cardiac dysfunction (log-rank P = 0.205). The ROC curve showed that the cutoff value of ΔLVGLS was -13%.ConclusionSubclinical cardiac dysfunction can be detected using two-dimensional speckle-tracking imaging. LVGLS, RVGLS, and TAPSE are more sensitive indices for detection.Clinical trial registration[https://www.chictr.org.cn/showprojen.aspx?proj=27498], identifier [ChiCTR1800016216].
Radiomic analysis of enhanced CMR cine images predicts left ventricular remodeling after TAVR in patients with symptomatic severe aortic stenosis
Authors: He Wenzhang , Huang He , Chen Xiaoyi , Yu Jianqun , Liu Jing , Li Xue , Yin Hongkun , Zhang Kai , Peng Liqing 
Published: December 2022

ObjectiveThis study aimed to develop enhanced cine image-based radiomic models for non-invasive prediction of left ventricular adverse remodeling following transcatheter aortic valve replacement (TAVR) in symptomatic severe aortic stenosis.MethodsA total of 69 patients (male:female = 37:32, median age: 66 years, range: 47–83 years) were retrospectively recruited, and severe aortic stenosis was confirmed via transthoracic echocardiography detection. The enhanced cine images and clinical variables were collected, and three types of regions of interest (ROIs) containing the left ventricular (LV) myocardium from the short-axis view at the basal, middle, and apical LV levels were manually labeled, respectively. The radiomic features were extracted and further selected by using the least absolute shrinkage and selection operator (LASSO) regression analysis. Clinical variables were also selected through univariate regression analysis. The predictive models using logistic regression classifier were developed and validated through leave-one-out cross-validation. The model performance was evaluated with respect to discrimination, calibration, and clinical usefulness.ResultsFive basal levels, seven middle levels, eight apical level radiomic features, and three clinical factors were finally selected for model development. The radiomic models using features from basal level (Rad I), middle level (Rad II), and apical level (Rad III) had achieved areas under the curve (AUCs) of 0.761, 0.909, and 0.913 in the training dataset and 0.718, 0.836, and 0.845 in the validation dataset, respectively. The performance of these radiomic models was improved after integrating clinical factors, with AUCs of the Combined I, Combined II, and Combined III models increasing to 0.906, 0.956, and 0.959 in the training dataset and 0.784, 0.873, and 0.891 in the validation dataset, respectively. All models showed good calibration, and the decision curve analysis indicated that the Combined III model had a higher net benefit than other models across the majority of threshold probabilities.ConclusionRadiomic models and combined models at the mid and apical slices showed outstanding and comparable predictive effectiveness of adverse remodeling for patients with symptomatic severe aortic stenosis after TAVR, and both models were significantly better than the models of basal slice. The cardiac magnetic resonance radiomic analysis might serve as an effective tool for accurately predicting left ventricular adverse remodeling following TAVR in patients with symptomatic severe aortic stenosis.
Could VGF and/or its derived peptide act as biomarkers for the diagnosis of neurodegenerative diseases: A systematic review
Authors: Alqarni Saleha , Alsebai Mashael 
Published: December 2022

BackgroundThe increasing ageing population has led to an increase in the prevalence of neurodegenerative diseases, such as Alzheimer’s disease (AD), Parkinson’s disease (PD), and amyotrophic lateral sclerosis (ALS). However, as yet, there are no simple biomarkers to predict the onset of such diseases. Recently, VGF and its peptides have been highlighted in neurodegenerative diseases. VGF (non-acronymic) is a polypeptide induced in PC12 cells by neurotrophic factors.ObjectiveThis systematic review aimed to determine whether VGF and/or its derived peptides can be used as biomarkers for the diagnosis of ALS, PD, and AD with specific attention to (1) the levels of VGF and/or its derived peptides, (2) amyloid-beta, (3) dopamine, and (4) cognitive score.MethodologyA search was undertaken in the Ovid EMBASE, Cochrane Library, PubMed, Scopus, and Web of Science for observational studies. Publications that assessed the level of VGF and/or its derived peptides among people with neurodegenerative diseases and compared them with healthy people were included. The quality of the included studies was assessed using the National Heart, Lung, and Blood Institute Quality Assessment Tool.ResultA search of the databases yielded 834 studies, of which, eight observational studies met the inclusion criteria with a total of 673 participants (51.7% males) aged &gt;18 years. Seven studies showed significant decreases in VGF and its derived peptides in adults with AD, PD, and ALS compared to healthy controls (p&lt;0.05). However, one study showed that there was no significant difference in VGF in AD compared to healthy control(p&gt;0.05). Furthermore, only one study reported that VGF levels were positively correlated with those of tissue dopamine but not with Aβ1-42, and low levels of VGF were associated to cognitive deficits.ConclusionThe use of VGF and its derivatives for the diagnosis of PD, ALS, AD remains unclear, so further investigation of the role of VGF in neurodegenerative diseases and pathophysiology is needed to provide new insights.