bluuebunny/crossref_metadata_2025_split huggingface dataset:
Iron (hydr)oxide formation in Andosols under extreme climate conditions
Authors: Klaes Björn , Thiele-Bruhn Sören , Wörner Gerhard , Höschen Carmen , Mueller Carsten W. , Marx Philipp , Arz Helge Wolfgang , Breuer Sonja , Kilian Rolf 
Published: February 2023

AbstractRedox-driven biogeochemical cycling of iron plays an integral role in the complex process network of ecosystems, such as carbon cycling, the fate of nutrients and greenhouse gas emissions. We investigate Fe-(hydr)oxide (trans)formation pathways from rhyolitic tephra in acidic topsoils of South Patagonian Andosols to evaluate the ecological relevance of terrestrial iron cycling for this sensitive fjord ecosystem. Using bulk geochemical analyses combined with micrometer-scale-measurements on individual soil aggregates and tephra pumice, we document biotic and abiotic pathways of Fe released from the glassy tephra matrix and titanomagnetite phenocrysts. During successive redox cycles that are controlled by frequent hydrological perturbations under hyper-humid climate, (trans)formations of ferrihydrite-organic matter coprecipitates, maghemite and hematite are closely linked to tephra weathering and organic matter turnover. These Fe-(hydr)oxides nucleate after glass dissolution and complexation with organic ligands, through maghemitization or dissolution-(re)crystallization processes from metastable precursors. Ultimately, hematite represents the most thermodynamically stable Fe-(hydr)oxide formed under these conditions and physically accumulates at redox interfaces, whereas the ferrihydrite coprecipitates represent a so far underappreciated terrestrial source of bio-available iron for fjord bioproductivity. The insights into Fe-(hydr)oxide (trans)formation in Andosols have implications for a better understanding of biogeochemical cycling of iron in this unique Patagonian fjord ecosystem.
Agricultural dust derived bacterial extracellular vesicle mediated inflammation is attenuated by DHA
Authors: Heires Art J. , Samuelson Derrick , Villageliu Daniel , Nordgren Tara M. , Romberger Debra J. 
Published: February 2023

AbstractDietary long-chain omega-3 polyunsaturated fatty acids (n-3 PUFA) and their pro-resolving metabolites are protective against atherosclerotic disease, and ameliorate systemic inflammatory conditions including lupus erythematosus, psoriasis, and bronchial asthma. Organic bioaerosol inhalation is a common and injurious hazard associated with agricultural occupations such as work in swine concentrated animal feeding operations (CAFOs) and is known to increase the risk for developing respiratory conditions such as asthma and COPD. Nearly all cells secrete membrane-bound vesicles (extracellular vesicles, EVs) that have the capacity to transmit protein, nucleic acid, and lipid signaling mediators between cells. Using a polymer-based isolation technique (ExoQuick, PEG) followed by ultracentrifugation, EVs were isolated from CAFO dust extracts, and were quantified and partially characterized. Here, we investigated the role of the n-3 PUFA docosahexaenoic acid (DHA) as a component of n-6 to n-3 PUFA mixtures used to recapitulate physiologically relevant dietary ratios in the resolution of inflammatory injury caused by exposure to EVs carried by agricultural organic dust in vitro. Primary human bronchial epithelial cells, fibroblasts and monocyte-derived macrophages were exposed to EVs isolated from swine CAFO dust. Cells were treated with mixtures of n-6 and n-3 PUFA during recovery from the EV-induced injury. CAFO dust extract (DE) was found to contain EVs that contributed significantly to the overall consequences of exposure to complete DE. DHA-rich PUFA ratios inhibited DE-derived EV-induced proinflammatory cytokine release dose-dependently. DHA-rich PUFA ratios also reversed the damaging effects of EVs on recellularization of lung matrix scaffolds, accelerated wound healing, and stimulated the release of pro-resolution mediators. These results underscore the importance of n-3 PUFA as anti-inflammatory compounds during recovery from EV-laden environmental dust exposure in the context of cellular responses in vitro, warranting future translational studies.
Nicotinamide adenine dinucleotide metabolism and arterial stiffness after long-term nicotinamide mononucleotide supplementation: a randomized, double-blind, placebo-controlled trial
Authors: Katayoshi Takeshi , Uehata Sachi , Nakashima Noe , Nakajo Takahisa , Kitajima Natsuko , Kageyama Masakatsu , Tsuji-Naito Kentaro 
Published: February 2023

AbstractMany animal studies have shown that oral administration of the nicotinamide adenine dinucleotide (NAD+) precursor nicotinamide mononucleotide (NMN) prevents the reduction of NAD+ levels in organs and tissues, helping alleviate aging-related diseases. However, there are very few clinical reports of NMN supplementation in humans. Thus, this study aimed to investigate the influence of a 12-week NMN oral supplementation on biochemical and metabolic health parameters. A 12-week randomized, double-blind, placebo-controlled, parallel-group clinical trial was conducted. A total of 36 healthy middle-aged participants received one capsule of either 125 mg NMN or placebo twice a day. Among the NAD+ metabolites, the levels of nicotinamide in the serum were significantly higher in the NMN intake group than in the placebo group. Pulse wave velocity values indicating arterial stiffness tended to decrease in the NMN intake group. However, no significant difference was found between the two groups. Long-term NMN supplementation at 250 mg/day was well tolerated and did not cause adverse events. NMN safely and effectively elevated NAD+ metabolism in healthy middle-aged adults. Additionally, NMN supplementation showed potential in alleviating arterial stiffness.
Association between daily gait speed patterns and cognitive impairment in community-dwelling older adults
Authors: Seo Kanako , Takayanagi Naoto , Sudo Motoki , Yamashiro Yukari , Chiba Ippei , Makino Keitaro , Lee Sangyoon , Niki Yoshifumi , Shimada Hiroyuki 
Published: February 2023

AbstractGait speed over a short distance is associated with cognitive impairment in older adults. Recently, daily gait speed has been assessed using accelerometers. However, because daily gait speed is only weakly correlation with gait speed over a short distance, its association with cognitive impairment needs to be investigated. The present study compared the daily gait speed patterns of normal cognition (NC), mild cognitive impairment (MCI), and general cognitive impairment (GCI) subjects measured every 3 h for two weeks using accelerometers. A total of 1959 participants were classified into the NC (N = 1519), MCI (N = 353), and GCI groups (N = 87). The results showed that the average daily gait speed of the GCI group was significantly lower than that of the NC group (p = 0.03). Furthermore, the average daily gait speeds of the MCI and NC groups were the same. However, the average daily gait speed of the MCI group during a specific time (12–15 o'clock) was significantly lower than that of the NC group (p < 0.01). These results suggest that changes in daily patterns may be detected by measuring daily gait speed, which depends on the degree of cognitive function.
Phytohormones regulate the non-redundant response of ω-3 fatty acid desaturases to low temperatures in Chorispora bungeana
Authors: Shi Yulan , Yang Sizhong , Zhao Zhixing , An Lizhe 
Published: February 2023

AbstractTo explore the contributions of ω-3 fatty acid desaturases (FADs) to cold stress response in a special cryophyte, Chorispora bungeana, two plastidial ω-3 desaturase genes (CbFAD7, CbFAD8) were cloned and verified in an Arabidopsis fad7fad8 mutant, before being compared with the microsomal ω-3 desaturase gene (CbFAD3). Though these genes were expressed in all tested tissues of C. bungeana, CbFAD7 and CbFAD8 have the highest expression in leaves, while CbFAD3 was mostly expressed in suspension-cultured cells. Low temperatures resulted in significant increases in trienoic fatty acids (TAs), corresponding to the cooperation of CbFAD3 and CbFAD8 in cultured cells, and the coordination of CbFAD7 and CbFAD8 in leaves. Furthermore, the cold induction of CbFAD8 in the two systems were increased with decreasing temperature and independently contributed to TAs accumulation at subfreezing temperature. A series of experiments revealed that jasmonie acid and brassinosteroids participated in the cold-responsive expression of ω-3 CbFAD genes in both C. bungeana cells and leaves, while the phytohormone regulation in leaves was complex with the participation of abscisic acid and gibberellin. These results point to the hormone-regulated non-redundant contributions of ω-3 CbFADs to maintain appropriate level of TAs under low temperatures, which help C. bungeana survive in cold environments.
Coordinate value of the femoral head center estimated using those of the tip of the greater trochanter and lesser trochanter
Authors: Imai Norio , Kimura Keishi , Sakagami Atsushi , Nozaki Asami , Horigome Yoji , Suzuki Hayato 
Published: February 2023

AbstractSeveral studies have reported estimating the femoral head center (FC) from reference points on the pelvis; however, none have reported estimates obtained from those on the femur. In this cross-sectional study, we investigated the estimated point of FC from the coordinate value of the tip of the greater trochanter (GT) and lesser trochanter (LT) using a formula with a three-dimensional measurement technique. We used data from 92 healthy Japanese subjects without any back or knee symptoms and no abnormalities in the hip, knee, or spine on plain radiographs. In our study, the difference in the anteroposterior direction was larger than that in the other directions. We speculate that the accuracy of defining the tip of the LT is difficult in the anteroposterior direction. Moreover, the correlation coefficients were larger for women. The reason for this was unclear because the variation in the proximal femur may be similar in women. We found that the average difference between the actual and calculated values was approximately 2 mm. We considered that the coordinate value of the FC from the tip of the GT could be estimated more accurately using the regression equation compared to previous methods based on pelvic reference points.
AKT/mTOR signaling modulates resistance to endocrine therapy and CDK4/6 inhibition in metastatic breast cancers
Authors: Abu-Khalaf Maysa M. , Alex Hodge K. , Hatzis Christos , Baldelli Elisa , El Gazzah Emna , Valdes Frances , Sikov William M. , Mita Monica M. , Denduluri Neelima , Murphy Rita , Zelterman Daniel , Liotta Lance , Dunetz Bryant , Dunetz Rick , Petricoin Emanuel F. , Pierobon Mariaelena 
Published: February 2023

AbstractEndocrine therapy (ET) in combination with CDK4/6 inhibition is routinely used as first-line treatment for HR+/HER2− metastatic breast cancer (MBC) patients. However, 30–40% of patients quickly develop disease progression. In this open-label multicenter clinical trial, we utilized a hypothesis-driven protein/phosphoprotein-based approach to identify predictive markers of response to ET plus CDK4/6 inhibition in pre-treatment tissue biopsies. Pathway-centered signaling profiles were generated from microdissected tumor epithelia and surrounding stroma/immune cells using the reverse phase protein microarray. Phosphorylation levels of the CDK4/6 downstream substrates Rb (S780) and FoxM1 (T600) were higher in patients with progressive disease (PD) compared to responders (p = 0.02). Systemic PI3K/AKT/mTOR activation in tumor epithelia and stroma/immune cells was detected in patients with PD. This activation was not explained by underpinning genomic alterations alone. As the number of FDA-approved targeted compounds increases, functional protein-based signaling analyses may become a critical component of response prediction and treatment selection for MBC patients.
Post-whaling shift in mating tactics in male humpback whales
Authors: Dunlop Rebecca , Frere Celine 
Published: February 2023

AbstractRecent studies have shown behavioural plasticity in mating strategies can increase a population’s ability to cope with anthropogenic impacts. The eastern Australian humpback whale population was whaled almost to extinction in the 1960s (~200 whales) and has recovered to pre-whaling numbers (>20,000 whales). Using an 18-year dataset, where the population increased from approximately 3,700 to 27,000 whales, we found that as male density increased over time, the use of mating tactics shifted towards more males engaging in non-singing physical competition over singing. Singing was the more successful tactic in earlier post-whaling years whereas non-singing behaviour was the more successful tactic in later years. Together, our study uncovers how changes in both local, and population-level male density resulted in a shift in the frequency, and fitness pay-off, of alternative mating tactics in a wild animal. This individual-level plasticity in male humpback whale mating tactics likely contributed to minimising their risk of extinction following a dramatic change in their social landscape due to whaling.
Cryo-EM structure and function of S. pombe complex IV with bound respiratory supercomplex factor
Authors: Moe Agnes , Ädelroth Pia , Brzezinski Peter , Näsvik Öjemyr Linda 
Published: February 2023

AbstractFission yeast Schizosaccharomyces pombe serves as model organism for studying higher eukaryotes. We combined the use of cryo-EM and spectroscopy to investigate the structure and function of affinity purified respiratory complex IV (CIV) from S. pombe. The reaction sequence of the reduced enzyme with O2 proceeds over a time scale of µs-ms, similar to that of the mammalian CIV. The cryo-EM structure of CIV revealed eleven subunits as well as a bound hypoxia-induced gene 1 (Hig1) domain of respiratory supercomplex factor 2 (Rcf2). These results suggest that binding of Rcf2 does not require the presence of a CIII-CIV supercomplex, i.e. Rcf2 is a component of CIV. An AlphaFold-Multimer model suggests that the Hig1 domains of both Rcf1 and Rcf2 bind at the same site of CIV suggesting that their binding is mutually exclusive. Furthermore, the differential functional effect of Rcf1 or Rcf2 is presumably caused by interactions of CIV with their different non-Hig1 domain parts.
On the impact of urbanisation on CO2 emissions
Authors: Luqman Muhammad , Rayner Peter J. , Gurney Kevin R. 
Published: February 2023

AbstractWe use a globally consistent, time-resolved data set of CO2 emission proxies to quantify urban CO2 emissions in 91 cities. We decompose emission trends into contributions from changes in urban extent, population density and per capita emission. We find that urban CO2 emissions are increasing everywhere but that the dominant contributors differ according to development level. A cluster analysis of factors shows that developing countries were dominated by cities with the rapid area and per capita CO2 emissions increases. Cities in the developed world, by contrast, show slow area and per capita CO2 emissions growth. China is an important intermediate case with rapid urban area growth combined with slower per capita CO2 emissions growth. Urban per capita emissions are often lower than their national average for many developed countries, suggesting that urbanisation may reduce overall emissions. However, trends in per capita urban emissions are higher than their national equivalent almost everywhere, suggesting that urbanisation will become a more serious problem in the future. An important exception is China, whose per capita urban emissions are growing more slowly than the national value. We also see a negative correlation between trends in population density and per capita CO2 emissions, highlighting a strong role for densification as a tool to reduce CO2 emissions.
A data-driven approach to rapidly estimate recovery potential to go beyond building damage after disasters
Authors: Loos Sabine , Lallemant David , Khan Feroz , McCaughey Jamie W. , Banick Robert , Budhathoki Nama , Baker Jack W. 
Published: February 2023

AbstractFollowing a disaster, crucial decisions about recovery resources often prioritize immediate damage, partly due to a lack of detailed information on who will struggle to recover in the long term. Here, we develop a data-driven approach to provide rapid estimates of non-recovery, or areas with the potential to fall behind during recovery, by relating surveyed data on recovery progress with data that would be readily available in most countries. We demonstrate this approach for one dimension of recovery—housing reconstruction—analyzing data collected five years after the 2015 Nepal earthquake to identify a range of ongoing social and environmental vulnerabilities related to non-recovery in Nepal. If such information were available in 2015, it would have exposed regional differences in recovery potential due to these vulnerabilities. More generally, moving beyond damage data by estimating non-recovery focuses attention on those most vulnerable sooner after a disaster to better support holistic and nuanced decisions.
Clinician Perspectives of Telehealth Pre–COVID-19: A Systematic Review and Qualitative Metasynthesis
Authors: Douglass Hannah , Lowman Joneen , Causey-Upton Renee 
Published: February 2023

Purpose:
The purpose of this study was to describe clinicians' attitudes and beliefs about telehealth prior to the COVID-19 pandemic and establish a baseline measure for change.

Method:
This study is a qualitative systematic review and metasynthesis, using the Enhanced Transparency in Reporting the Synthesis of Qualitative Research guideline. Seven databases were searched. Studies were included if they used interviews or focus groups, included at least one or more rehabilitation professions in the sample, and addressed attitudes toward telehealth as a service delivery model.

Results:
Nine studies met the inclusion criteria. Data were extracted and coded by the first author using thematic synthesis. Six primary themes were formed: (a) constant comparison of telehealth with in-person guides attitude and acceptance, (b) telehealth presents barriers and opportunities for communication, (c) lack of telehealth education and training impacts clinician confidence, (d) the flexibility of telehealth changes service delivery options and access for patients, (e) the telehealth environment creates challenges with safety and engagement, and (f) technical components and support are influential to the telehealth delivery process.

Discussion:
This study provides evidence that clinician perceptions of telehealth are similar across disciplines within rehabilitation and other allied health fields. Views were generally positive. However, less experienced clinicians were more likely to have reservations about telehealth, indicating a need for more education and training. Clinical, research, and educational implications are discussed. It is recommended that these themes be utilized to support methods for increasing clinician acceptance of telehealth and achieving sustainable telehealth programs. The overall strength of the recommendation is moderate.

Supplemental Material:

https://doi.org/10.23641/asha.21992576
Impedance learning adaptive super‐twisting control of a robotic exoskeleton for physical human‐robot interaction
Authors: Brahmi Brahim , Rahman Mohammad Habibur , Saad Maarouf 
Published: February 2023

AbstractThis study addresses two issues about the interaction of the upper limb rehabilitation robot with individuals who have disabilities. The first step is to estimate the human's target position (also known as TPH). The second step is to develop a robust adaptive impedance control mechanism. A novel Non‐singular Terminal Sliding Mode Control combined with an adaptive super‐twisting controller is being developed to achieve this goal. This combination's purpose is to provide high reliability, continuous performance tracking of the system's trajectories. The proposed adaptive control strategy reduces matched dynamic uncertainty while also lowering chattering, which is the sliding mode's most glaring issue. The proposed TPH is coupled with adaptive impedance control with the use of a Radial Basis Function Neural Network, which allows a robotic exoskeleton to simply track the desired impedance model. To validate the approach in real‐time, an exoskeleton robot was deployed in controlled experimental circumstances. A comparison study has been set up to show how the adaptive impedance approach proposed is better than other traditional controllers.
Imaging of the internal structure of an asteroid analogue from quasi-monostatic microwave measurement data
Authors: Sorsa Liisa-Ida , Yusuf Yusuf Oluwatoki , Dufaure Astrid , Geffrin Jean-Michel , Eyraud Christelle , Pursiainen Sampsa 
Published: February 2023

Context.The internal structures of small solar system bodies (SSSBs) are still poorly understood. In this paper, we find an experimental tomographic reconstruction of coarse high-contrast details inside a complex-structured target object using multipoint full-wave radar data.Aims.Our aim is to advance the development of inversion techniques to be used in potential planetary scientific radar investigations targeting SSSBs, which have complex shapes and whose internal structure is largely unknown. Finding out the structure is an important scientific objective of Solar System research in order to understand its history and evolution.Methods.This is the second part (Paper II) of a joint study considering the methods to analyse and invert quasi-monostatic microwave measurement data of an asteroid analogue. We focused on incorporating advanced, full-wave, forward simulation in time domain with experimental data obtained from multiple measurement points. In particular, this study investigates multiple scattering and multipath effect suppression (MES) to reduce artefacts in the reconstructions. MES is necessary since the high-contrast and complex-shaped target and, especially, its back wall in high curvature regions cause intense reflections that deteriorate the reconstruction quality if not treated correctly. We considered the following two approaches to obtain MES: (i) geometrical optics-based pathlength thresholding and (ii) a peak detection method to investigate whether a data-driven approach could be used. At the inversion stage, we investigated marginalisation of random effects due to modelling by splitting a larger point set into several sparse sets of measurements.Results.Based on the results, MES is crucial to localise a void inside the complex analogue target. A reconstruction can be found when the maximum signal propagation time approximately matches that of the first back-wall echo for each measurement point. The marginalisation approach allows us to find a reconstruction that is comparable in quality to the case of full data, while reducing the computation effort per subsystem, which is advantageous when inverting a large data set.