bluuebunny/crossref_metadata_2025_split huggingface dataset:
A retrospective study of lipid profile during the various phases of timing in renal failure and hypothyroid patients
Authors: Eadala Suresh , Muthireddy Latha Rani , Vangara Durga Prasad , Mahavadi Srihita 
Published: July 2023

: As there are very few studies in our region about the metabolic changes in hypothyroid and renal failure patients in collection of sample during various phases of time, where these organs were involved in regulating this metabolism.To evaluate the trends of lipid profiles in hypothyroidism and chronic kidney disease throughout various phases of time. This was a retrospective control study conducted on 50 CKD patients, 50 hypothyroidism patients and 50 controls in the tertiary care hospital attending to OPD. Collected samples are analyzed for Total cholesterol, Triglycerides, and HDL cholesterol whereas LDL and VLDL are measured by calculated method taken at various phases of time. The data was entered into the master chart and analyzed statistically.: It was found that random lipid profile values in CKD and hypothyroidism were higher and are statistically significant compared to fasting and postprandial states. Particularly there is an elevation in Total cholesterol, Triglycerides, LDL cholesterol whereas HDL levels are decreased.: This research work is done by analyzing the fasting, postprandial and random states because it includes an explanation of why a random blood sample is preferred to analyze lipid profile values. Thyroid disease and renal failure patients are more prone to development of atherosclerosis which were primary cause for development of cardiovascular and cerebrovascular complications. So collection of the sample also plays key role in management.
Study of fingerprint patterns in relation to gender and blood groups
Authors: Pratinidhi S. A. , Lunawat Vaishali , Tilokchandani Mohak , Bhujbal Chaitanya , Shejul Rutuja , Sahoo Madhumita 
Published: July 2023

Dermatoglyphics (fingerprint/dactylography) is derived from the Greek word “Derma=Skin, Glyph=Carve.” Dermatoglyphics is defined as the scientific study of naturally occurring epidermal ridges and their configuration on the digits, palms and soles apart from flexion crease and secondary folds. The objective of the study was to determine correlation if any exits between the fingerprint pattern and blood group and gender. The general study design selected for this article was cross-sectional study. Here, the primary outcome variable is to determine correlation (if any) between the fingerprint pattern and blood group and gender. A total of 271 students volunteered for the study. Loops were found to the most prevalent type of fingerprint in all blood groups. Blood groups and primary fingerprint patterns were found to be associated with the association being statistically significant. The correlation between the said entities would enable better authentication and prediction of identity of a person and thereby has the potential for great application in the field of forensics.
Association between serum alpha-fetoproteinlevel and liver function parameters in hepatocellular carcinoma patients: A case-control study
Authors: Varma Poornima R 
Published: July 2023

: Hepatocellular carcinoma (HCC) is the most common type of primary liver cancer occurs in people with liver diseases like chronic hepatitis B and hepatitis C virus. Early diagnosis and monitoring of HCC is possible with the help of tumor marker AFP. The aim of the study was to assess whether AFP level is associated with selected liver function tests in hepatocellular carcinoma patients.: Total 90 individuals are selected, 50 HCC patients with AFP &#62;20ng/ml and 40 subjects (normal LFT and AFP value &#60;10ng/ml) with an age between 40-70. Serum is used for analysis of AFP, SGOT, SGPT, ALP by Maglumi 1000 and Siemen biochemistry analyzer (using principle Chemiluminescence immunoassay) respectively. Unpaired T test (IBM SPSS 22.0) were used. Elevated levels of AFP (p value &#60;0.05), SGOT (p value &#60; 0.001), SGPT (p value &#60; 0.001), ALP (p value &#60; 0.001) shows statistical significance within study population and also indicated the risk of developing HCC with respect to AFP level in liver disease patients with elevated LFT parameters.: From the study, it is concluded that LFT and AFP are comparatively elevated in HCC. Treatment is usually not possible with advanced HCC due to failure of not doing proper screening at appropriate time. Early diagnosis and monitoring of HCC are possible with the help of tumor marker AFP.
Assessment of extracted DNA from whole blood and buffy coat in healthy individuals
Authors: Kumar Umesh , Singh Khushdeep , Singh Jaspreet 
Published: July 2023

Deoxyribonucleic acids (DNA) are the genetic material in all organisms. Extraction of DNA is the initial gait for the field of molecular biotechnology and biomedical research to advance understanding of human illnesses from various diseases and provide practical solutions for diagnosis and treatment. Routine diagnostic techniques in medicine have been greatly enhanced by molecular biology. Due to research on deoxyribose nucleic acid, it frequently finds use in medicine. Efficient isolation of DNA from a sample is the basis for successful forensic DNA profiling. DNA can be extracted from variety of samples such as a whole blood, buffy coat, hair, skin, tissue, urine, buccal swab, saliva etc. Numerous molecular biologic applications require genomic DNA (gDNA) in high quality and in adequate quantity. We extracted genomic DNA from buffy coat and whole blood samples and compared the results of the product obtained in terms of quantity (concentration of DNA extracted and DNA obtained per ml of blood used) and quality (260nm/280nm ratio) of the obtained yield. The aim of this study was to extract DNA from whole blood and buffy coat and compare quantity or Concentration (μg/ml) and purity (260nm/280nm) of extracted DNA. The present study was a hospital- based comparative and experimental study. The study was conducted over a period of 1 year on 50 samples. The venous blood sample is taken from the healthy individuals who are willing to donate blood in blood bank. The 5 ml of blood sample taken by venipuncture from healthy individuals were equally dividing into two anticoagulant EDTA tubes by equal volume. The first tube was use as it is, as a whole blood sample and second tube were centrifuged to obtained three phase separation and prepare buffy coat. From both of the samples extraction of DNA is carried out by follow the protocols of modified salting out method. The integrity and purity of the extracted DNA are assessed by Thermo Scientific Nano Drop 2000Spectrophotometers. Our results show thatMean±SD of concentration of DNA from whole blood and buffy coat was 37.49±25.31 μg/ml and 101.83±31.22 μg/ml (P &#60; 0.0001). The Mean±SD of purity (260nm/280nm) of DNA from whole blood and buffy coat was 1.66±0.16 and 1.74±0.15 (p≤0.05). The both p value was found to be statistically significant.The present study concluded that DNA extracted from Buffy coat had purity as well as mean concentration significantly higher when compared to DNA extracted from whole blood.
Case report on nutrition management in liver transplant
Authors: Raj Edwina , Londe Pravalika , Sakpal Mallikarjun , Asthana Sonal , Khanam Simran 
Published: July 2023

Liver transplantation or hepatic transplantation is a procedure of replacing diseased liver with a healthy liver. It needs cautious post-operative care including nutritional intervention. We present a 65 year old patient underwent a liver transplant due to acute liver failure. The patient’s diet history revealed a daily intake of carbohydrates and fats but inadequate protein intake. The patient’s nutritional interventions were analyzed and evaluated through hospital recalls and proper follow up visits. In studies, hospital recall on 7 day of post-surgery showed a great improvement in calorie and protein intake. The discharge diet plan included a well-balanced diet with protein, carbohydrates and fat intake to ensure nutritional care for the patient’s recovery. Nutritional intervention plays a vital role in post-operative care of liver transplant patients. This case study signifies the importance of personalized dietary intervention to resolve PEM, improve nutritional intake while supporting successful surgical outcome and recovery .
EVALUATION OF THE EFFICIENCY OF THE OPTIONS FOR THE MODERNIZATION OF THE HEAT SUPPLY AND THERMAL PROTECTION OF THE OFFICE BUILDING
Authors: Deshko Valerii , Naumchuk Olena 
Published: July 2023

The concept of implementation of the state policy in the field of ensuring the energy efficiency of buildings in terms of increasing the number of buildings with close to zero energy consumption and the National Plan for the increase in the number of buildings with close to zero energy consumption are aimed at achieving the goals set out in the Energy Performance of Building Directive, which indicates significant steps towards improving the energy efficiency of the Ukrainian construction sector. According to the above regulations, a prerequisite for new construction and renovation of existing buildings is to ensure a high level of thermal insulation of the building and the use of renewable sources to cover energy consumption. The work carried out a feasibility study of the modernization of an office building in the city of Kyiv to the level of a highly energy-efficient building using a heat pump to provide the necessary heat supply. Estimation of generation of required heat supply of the building by the heat pump and used electrical energy by the heat pump is based on the simulation results in the GeoTSOL software environment. In order to assess the economic attractiveness of the project for upgrading an existing building to the level of a highly energy-efficient building using renewable sources for heat supply, the calculation of the energy demand for heating in the Design Builder software environment and the integral costs for heating the building using renewable and traditional energy sources at different levels of thermal protection was made. The results obtained indicate attractive, both from an economic and environmental point of view, the possibilities of implementing the construction of a highly energy-efficient building that uses renewable energy sources for heat supply.
DETERMINATION THE PARAMETERS OF STRESSED AND DEFORMED STATE A GONDOLA CAR AT DURING INTERACTION BY A ROTARY CAR DUMPER
Authors: Viznyak Ruslan 
Published: July 2023

In the article the specifics of force factors namely operating loads influencing durability of bodies of gondola cars at their unloading on stationary rotary car tippers are processed, possible characteristic damages and malfunctions at the same time are analyzed. Finite-element models of the structure are constructed and the stress-strain state is estimated. Recommendations for modernization by designing and strengthening those structural areas of the body that are subject to extreme exposure to operating loads, according to the design regime, which is due to the use of unloading on a car dumpers. Unloading of gondola cars in the method of overturning was introduced at industrial enterprises in the last century and is now successfully carried out with the help of stationary rotary wagon tippers. The task of the study of the stress-strain state of the body of the wagon, based on the features of the mutual operation of the mechanical system was solved by numerical integration of differential equations of distribution of full potential of potential energy on structural elements – applied variation methods of elasticity theory, theoretical and structural mechanics of machine-building constructions, and also resistance of materials whose basic laws are integrated into the most exact method for strength analysis. The maximum values of stresses in the structural zones of the side wall at the full cycle of overturning the body PV reach more than 800 MPa, which significantly exceeds the allowable for steel grades used in car building, and will inevitably lead to other deformations. This is especially observed at an angle of rotation of the system by 90 °, the maximum displacement is more than 60-70 mm. After careful research of technologies and technical means of unloading by the method of overturning the analysis concerning force influence of working bodies of car tippers on the basic elements of a bearing design of a body was carried out. The obtained results allowed to conclude that strengthening and adaptation of the body structure to the real conditions of unloading will directly reduce the number of damages and operational malfunctions, so these measures should primarily be taken in the design, development and construction of new generation gondola bodies unloading by rotary car dumpers or wagon tippers.
Body composition as an indicator of metabolic changes in mice obtained by <i>in vitro</i> fertilization
Authors: Anisimova M. V. , Gon Yanli , Kontsevaya G. V. , Romashchenko A. V. , Khotskin N. V. , Stanova A. K. , Gerlinskaya L. A. , Moshkin M. P. 
Published: July 2023

To identify body systems subject to epigenetic transformation during in vitro fertilization (IVF), comparative morphological and functional studies were performed on sexually mature offspring of outbred CD1 mice, specific-pathogen-free (SPF), obtained by IVF (experiment) and natural conception (control). The studies included assessment of age-related changes in body weight and composition, energy intake and expenditure, and glucose homeostasis. To level the effects caused by the different number of newborns in the control and in the experiment, the size of the fed litters was halved in the control females. Males obtained using the IVF procedure were superior in body weight compared to control males in all age groups. As was shown by analysis of variance with experiment/control factors, gender, age (7, 10 and 20 weeks), the IVF procedure had a statistically significant and unidirectional effect on body composition. At the same time, IVF offspring outperformed control individuals in relative fat content, but were behind in terms of lean mass. The effect of the interaction of factors was not statistically significant. IVF offspring of both sexes had higher fat to lean mass ratios (FLR). Since adipose tissue contributes significantly less to total energy intake compared to muscle, the main component of lean mass, it is not surprising that at the same level of IVF locomotor activity offspring consumed less food than controls. When converted to one gram of body weight, this difference reached 19 %. One of the consequences of reduced utilization of IVF energy substrates by offspring is a decrease in their tolerance to glucose loading. The integral criterion for the effectiveness of restoring the initial glucose level is the area under the curve (AUC), the value of which was 2.5 (males) and 3.2 (females) times higher in IVF offspring compared to the corresponding control. Thus, the totality of our original and literature data shows an increase in the risk of metabolic disorders in IVF offspring, which is confirmed by epidemiological studies of a relatively young cohort of people born using assisted reproductive technologies.
Genetic markers of children asthma: predisposition to disease course variants
Authors: Smolnikova M. V. , Kasparov Ed. W. , Malinchik M. A. , Kopylova K. V. 
Published: July 2023

Asthma is a heterogeneous and often difficult to treat condition that results in a disproportionate cost to healthcare systems. Children with severe asthma are at increased risk for adverse outcomes including medication-related side effects, life-threatening exacerbations, and impaired quality of life. An important therapeutic focus is to achieve disease control, which is supposed to involve a personalized approach to treatment of asthma of any severity. Asthma is a multifactorial disease with a significant genetic determinant, however, the inheritance of asthma has not been fully elucidated. Polymorphic genes of inflammatory mediators, including cytokines, play an important role in developing various disease forms. In the current study, large-scale original data on the prevalence of cytokine gene genotypes (IL2, IL4, IL5, IL6, IL10, IL12, IL13, IL17A, IL31, IL33, IFNG, TNFA) among Russian children with asthma in Krasnoyarsk region have been obtained. Genotyping was carried out using real-time PCR. We identified markers predisposing to the development of different variants of the course of childhood asthma: the CT genotype and T allele of IL4 rs2243250 are associated with asthma (p < 0.05), especially in mild asthma and in controlled asthma. The TT genotype and allele T of IL13 rs1800925 are associated with severe and uncontrolled asthma (p < 0.05). The AA genotype of IL17A rs2275913, the TT genotype of IFNG rs2069705 and allelic A variants of TNFA rs1800629 are associated with mild asthma, and the TT genotype of IFNG rs2069705 is additionally associated with controlled asthma. The results obtained will supplement information on the prevalence of polymorphic variants of the cytokine genes in the Russian population and in asthma patients with different disease courses, which is likely to be used in order to shape a plan for Public Health Authority to prevent the development of severe uncontrolled asthma and to optimize personalized therapy.
Obraz przewodnika górskiego w literaturze romantycznej. Część II
Authors: Kolbuszewska Ewa 
Published: July 2023

The article is a continuation of the analysis begun in the previous (15th) volume of Góry – Literatura – Kultura and dealing with the image of the mountain guide. In the second part the author discusses the earliest high mountain expeditions in the history of Tatra tourism, which were led by more or less experienced guides. The history of the profession in the Tatras goes back to the 17th century. One of the first people to employ a professional guide was a man named Simplicissimus (1654) — this was for a three-day high mountain trek. Among the subsequent expeditions, one of the biggest was a scientific expedition organised by Robert Townson (1793), who made an ascent of Łomnica, guided by Hans Gross.
Attempts to create an ideal image of the mountain guide were made in the first mountain guidebooks by Baltazar Hacquet, Eugeniusz Janota or Walery Eljasz. When the Tatra Society was established in 1873, it concluded that one of the most urgent matters to solve was the question of providing care for guides. It was to be regulated by a newly drafted statute, which created an organisational framework for the guiding profession, as a consequence of which guides were divided into three categories in accordance with their qualifi cations. A breakthrough in mountain tourism came in 1874, when Dr Tytus Chałubiński arrived in Zakopane and began his original “itinerary-less trips”, which lasted a few days. The numerous participants in these trips were led by the best guides. This irreverent way in which an elite group usurped the services of guides particularly experienced in mountain guiding provoked a heated discussion within the tourist community, which raged in the press at the time.
Coenzyme Q10, Vitamin E and Polyvitamin B: an exploratory double-blind randomized cross-over study in Phelan-McDermid Syndrome
Authors: Persico Antonio M. , Ricciardello Arianna , Cucinotta Francesca , Turriziani Laura , Calabrese Giorgia , Tomaiuolo Pasquale , Bella Tiziana Di , Bellomo Fabiana , Boncoddo Maria , Turturo Giada , Mirabelli Silvestro , Asta Lisa , Banchelli Federico , Costantini Riccardo Cuoghi , D'Amico Roberto 
Published: July 2023

Aim: Defective mitochondrial function and increased oxidative stress have been documented in Autism Spectrum Disorder (ASD) and Phelan-McDermid syndrome (PMS), one of the best-known monogenic forms of ASD. The purpose of this exploratory, double-blind, randomized cross-over trial (RCT) is to verify the efficacy and safety of a “metabolic support therapy” (MST) in PMS, while defining the experimental methodology most apt at maximizing sensitivity and reliability.
Methods: A total of 31 PMS patients completed 4 months of Coenzyme Q10 (50/100 mg b.i.d.) + vitamin E (30/60 mg/d) + polyvitamin B ("active compound") vs. 4 months of only Vitamins E and B ("active comparator"). To explore their sensitivity and reliability, four primary outcome measures were used: VABS, CARS, CGI-I, and VAS. Secondary outcome measures span adaptive behaviors, social cognition, autism, problem behaviors, quality of life (QoL), communication, and comorbidities.
Results: CoQ10+vit. E and B yielded significantly greater improvement in several measures of cognition and adaptive functioning, motor skills, and stereotypic behaviors compared to vit. E and B only. Maternal QoL was especially improved in the presence of CoQ10 (P < 0.004). Time x Treatment interactions in CGI-I and VAS "restricted interests" scores support positive contributions also by vitamins E and B. Side effects, including hyperactivity, insomnia, and irritability, were mild, rare, and did not differ between treatment periods.
Conclusion: MST may produce small-to-moderate improvement, especially in motor skills, social motivation, adaptive behaviors, responsiveness to environmental stimulation, and stereotypic behaviors in up to approximately 70% of PMS patients. A targeted confirmatory RCT contrasting Q10+vit E and B vs. inactive placebo is now warranted.
From non-problematic smartphone use to smartphone addiction: Impulsivity-based profiles
Authors: Sánchez-Kuhn Ana , L. Puga Jorge , Flores Pilar , Ruiz-Ruano Ana María 
Published: July 2023

Problematic smartphone use (PSU) is an uncontrollable behavior that impedes the ability to stop using it despite potential negative consequences. This excessive behavior has been considered in the research field as a behavioral addiction, as literature has shown common characteristics with behavioral addictions, including its impulsivity-driven factor. However, impulsivity is a multidimensional construct whose specific traits lead differently to each addiction. Hence, the present study aimed to address the different existent PSU profiles depending on their individual impulsivity personality traits. To this end, N = 412 adults (average age 31.91, SD = 11.70, including 108 men and 304 women) were recruited to explore their daily smartphone usage, level of smartphone addiction (SAS-SV) and impulsivity profile across the five impulsivity personality traits (UPPS-P). Cluster analysis revealed the existence of three different profiles: one without PSU; one showing an excessive impulsively but almost no addictive smartphone use, expressing only a loss of control symptomatology; and one showing excessive impulsively but also addictive smartphone patterns, driven mainly by the impulsivity personality traits of negative urgency, positive urgency and lack of premeditation. Therefore, this study showed the impulsivity personality traits that differentiate excessive from addictive smartphone use, which is valuable information for the development of more precise prevention and interventions programs.