bluuebunny/crossref_metadata_2025_split huggingface dataset:
Using network meta-analysis to predict the percentage of missing participants for a future trial
Authors: Spineli Loukia M 
Published: March 2023

Background Using evidence synthesis to design a clinical trial has long been advocated as the key against research waste. However, the relevant methodology does not deal with possible missing participants (MP) that may occur in a future trial. We illustrated the synergism of the baseline effects model and network meta-analysis (NMA) to predict the percentage of MP for a future trial. Methods We considered the network of a published systematic review as a case study. We applied the baseline effects model, followed by the relative effects model using Bayesian methods to predict the percentage of MP in each intervention when conducting NMA and a series of pairwise meta-analyses. We illustrated the posterior distribution of the predicted percentage MP under both synthesis methods alongside the MP reported in the corresponding trials for each intervention. Results Selecting different interventions for the baseline effects model yielded different predicted baseline effects and led to different predicted percentages of MP for the remaining interventions, highlighting the need to carefully pre-specifying the intervention for the baseline effects model. Both synthesis methods provided almost identical posterior distributions of predicted percentage MP for estimating similar summary odds ratios. There was great variability in the percentage of MP across the trials for each intervention, manifesting as considerable variability in the percentage difference in MP compared to NMA. Conclusions Incorporating predictions and absolute effects in the context of MP in NMA aids in determining the anticipated percentage of MP in the compared interventions to plan a future trial efficiently.
Resection and Reconstruction of a Giant Paranasal Osteoma in a Pediatric Patient
Authors: Aycart Mario A. , Banwell Alison , Al-Shaqsi Sultan Z. , Kratky Vladimir , Wolter Nikolaus , Kulkarni Abhaya V. , Forrest Christopher R. , Riesel Johanna N. 
Published: March 2023

Study Design Case Report. Objective Paranasal osteomas are rare, benign bony tumors of the craniofacial skeleton and most are treated endoscopically without the need for reconstruction. In rare cases, open traditional craniofacial approaches are required with a paucity of literature describing extirpative maneuvers in this setting. We present a unique case of a rapidly growing, paranasal bony tumor in a pediatric patient and describe our surgical technique for resection and reconstruction. Methods A 13-year old male presented with eight months of progressive, painless right periorbital swelling and proptosis. Trans-nasal biopsy confirmed an osteoma of the right ethmoid sinus and endoscopic resection was recommended. Due to interval tumor growth, there was worsening visual acuity that precluded a minimally invasive approach. Pre-operative and post-operative 3D computed tomography (CT) scans were completed, and a 3D printed skull model was produced to aid in surgical planning. A multi-disciplinary team of Otolaryngology, Neurosurgery, and Plastic Surgery was employed. The majority of the extirpation was performed with a highspeed carbide burr. Results Post-operative course was unremarkable and CT scan demonstrated a complete resection. Pathology confirmed the diagnosis of an osteoma. Six-month follow-up demonstrated normal ophthalmologic function and visual acuity with excellent aesthetic results. Conclusions This is a rare, complex presentation of a paranasal osteoma that was successfully managed with an open, multi-disciplinary approach. We highlight pearls gleaned from this extirpative and reconstructive technique. Due to the tumor density and location, a multidisciplinary team, Stealth navigation, an open, intra- and extra-cranial approach and use of a highspeed carbide burr were essential.
Vascular injury during laparoscopic partial nephrectomy in a solitary kidney: management, outcome and audit
Authors: Venkatachalapathy Vigneswara srinivasan Sockkalingam , Palathullil Datson George , Abraham George Palathullil 
Published: March 2023

Abstract
Background
Vascular injury during laparoscopic partial nephrectomy is a dreadful complication. Though it is a commonly discussed complication, the literature on the circumstances leading to vascular injury, methods of managing the complication and reporting of final outcomes in those cases are limited. We report a case of vascular injury during laparoscopic partial nephrectomy for a hilar tumor in a solitary kidney. We highlight the management of the complication, present the outcome and review the surgical technique.

Case presentation
A 62-year-old male with solitary kidney presented with left renal hilar mass of size 4.4 × 3.8x3.6 cm. The renal nephrometry score was 10ph. The serum creatinine at the time of presentation to the hospital was 1.4 mg/dl. Laparoscopic partial nephrectomy was performed. The patient had severe intraoperative bleeding due to a segmental renal artery injury. The bleeding presented after hilar unclamping and was managed by intracorporeal vascular repair. The blood loss was around 500 ml. The postoperative period was uneventful without the need for hemodialysis. The histopathology report was suggestive of clear cell renal cell carcinoma with negative surgical margin. The follow-up magnetic resonance urogram did not show evidence of any arterial pseudoaneurysm or residual/recurrent tumor. At 18-month follow-up, the serum creatinine was 1.9 mg/dl and the patient did not have any complaints.

Conclusions
Complication of vascular injury while performing laparoscopic partial nephrectomy for complex hilar tumors should be anticipated beforehand. Contingency plans to tackle this complication must be in place before attempting the surgery. Intracorporeal repair of vascular injury during laparoscopic partial nephrectomy is feasible when expertise is available. Low threshold for using endoscopic ultrasound, employing cold ischemia techniques, careful usage of hot cut during tumor resection and complete defatting of the kidney can all be considered ‘safe surgical practices’ during laparoscopic partial nephrectomy for complex hilar tumors. Following aforementioned ‘safe surgical practices’ helps in improving the outcomes and reducing the possibility of complication of vascular injury and helps in managing the complication effectively if it happens despite the precautions.
Evolutionary relevance of single nucleotide variants within the forebrain exclusive human accelerated enhancer regions
Authors: Khatoon Hizran , Raza Rabail Zehra , Saleem Shoaib , Batool Fatima , Arshad Saba , Abrar Muhammad , Ali Shahid , Hussain Irfan , Shubin Neil H. , Abbasi Amir Ali 
Published: March 2023

Abstract
Background
Human accelerated regions (HARs) are short conserved genomic sequences that have acquired significantly more nucleotide substitutions than expected in the human lineage after divergence from chimpanzees. The fast evolution of HARs may reflect their roles in the origin of human-specific traits. A recent study has reported positively-selected single nucleotide variants (SNVs) within brain-exclusive human accelerated enhancers (BE-HAEs) hs1210 (forebrain), hs563 (hindbrain) and hs304 (midbrain/forebrain). By including data from archaic hominins, these SNVs were shown to be Homo sapiens-specific, residing within transcriptional factors binding sites (TFBSs) for SOX2 (hs1210), RUNX1/3 (hs563), and FOS/JUND (hs304). Although these findings suggest that the predicted modifications in TFBSs may have some role in present-day brain structure, work is required to verify the extent to which these changes translate into functional variation.

Results
To start to fill this gap, we investigate the SOX2 SNV, with both forebrain expression and strong signal of positive selection in humans. We demonstrate that the HMG box of SOX2 binds in vitro with Homo sapiens-specific derived A-allele and ancestral T-allele carrying DNA sites in BE-HAE hs1210. Molecular docking and simulation analysis indicated highly favourable binding of HMG box with derived A-allele containing DNA site when compared to site carrying ancestral T-allele.

Conclusion
These results suggest that adoptive changes in TF affinity within BE-HAE hs1210 and other HAR enhancers in the evolutionary history of Homo sapiens might have brought about changes in gene expression patterns and have functional consequences on forebrain formation and evolution.

Methods
The present study employ electrophoretic mobility shift assays (EMSA) and molecular docking and molecular dynamics simulations approaches.
Potential key genes involved in metabolic resistance to malathion in the southern house mosquito, Culex quinquefasciatus, and functional validation of CYP325BC1 and CYP9M12 as candidate genes using RNA interference
Authors: Huang Xinyue , Kaufman Phillip E. , Athrey Giridhar N. , Fredregill Chris , Alvarez Christina , Shetty Vinaya , Slotman Michel A. 
Published: March 2023

Abstract
Background
Metabolic detoxification is one of the major mechanisms contributing to the development of resistance in mosquitoes, including the southern house mosquito, Culex quinquefasciatus. The three major detoxification supergene families, cytochrome P450s, glutathione S-transferases and general esterases, have been demonstrated to play an important role in metabolic resistance. In this study, we performed differential gene expression analysis based on high-throughput transcriptome sequencing on samples from four experimental groups to give insight into key genes involved in metabolic resistance to malathion in Cx. quinquefasciatus. We conducted a whole transcriptome analysis of field captured wild Cx. quinquefasciatus from Harris County (WI), Texas and a malathion susceptible laboratory-maintained Sebring colony (CO) to investigate metabolic insecticide resistance. Field captured mosquitoes were also phenotypically classified into the malathion resistant and malathion susceptible groups following a mortality response measure conducted using a Centers for Disease Control and Prevention (CDC) bottle assay. The live (MR) and dead (MS) specimens from the bottle assay, along with an unselected WI sample and a CO sample were processed for total RNA extraction and subjected to whole-transcriptome sequencing.

Results
We demonstrated that the genes coding for detoxification enzymes, particularly cytochrome P450s, were highly up-regulated in the MR group compared to the MS group with similar up-regulation observed in the WI group compared to the CO group. A total of 1,438 genes were differentially expressed in comparison between MR and MS group, including 614 up-regulated genes and 824 down-regulated genes. Additionally, 1,871 genes were differentially expressed in comparison between WI and CO group, including 1,083 up-regulated genes and 788 down-regulated genes. Further analysis on differentially expressed genes from three major detoxification supergene families in both comparisons resulted in 16 detoxification genes as candidates potentially associated with metabolic resistance to malathion. Knockdown of CYP325BC1 and CYP9M12 using RNA interference on the laboratory-maintained Sebring strain significantly increased the mortality of Cx. quinquefasciatus after exposure to malathion.

Conclusion
We generated substantial transcriptomic evidence on metabolic detoxification of malathion in Cx. quinquefasciatus. We also validated the functional roles of two candidate P450 genes identified through DGE analysis. Our results are the first to demonstrate that knockdown of CYP325BC1 and CYP9M12 both significantly increased malathion susceptibility in Cx. quinquefasciatus, indicating involvement of these two genes in metabolic resistance to malathion.
Investigation of antimicrobial resistance patterns and molecular typing of Pseudomonas aeruginosa isolates among Coronavirus disease-19 patients
Authors: Shiralizadeh Somaye , Keramat Fariba , Hashemi Seyyed Hamid , Majzoobi Mohammad Mehdi , Azimzadeh Masoud , Alikhani Mohammad Sina , Karami Pezhman , Rahimi Zahra , Alikhani Mohammad Yousef 
Published: March 2023

Abstract
Background
Pseudomonas aeruginosa is a common co-infecting pathogen recognized among COVID-19 patients. We aimed to investigate the antimicrobial resistance patterns and molecular typing of Pseudomonas aeruginosa isolates among Coronavirus disease-19 patients.

Methods
Between December 2020 and July 2021, 15 Pseudomonas aeruginosa were isolated from COVID-19 patients in the intensive care unit at Sina Hospital in Hamadan, west of Iran. The antimicrobial resistance of the isolates was determined by disk diffusion and broth microdilution methods. The double-disk synergy method, Modified Hodge test, and polymerase chain reaction were utilized to detect Pseudomonas aeruginosa extended spectrum beta-lactamase and carbapenemase producers. Microtiter plate assay was performed to evaluate the biofilm formation ability of the isolates. The isolates phylogenetic relatedness was revealed using the multilocus variable-number tandem-repeat analysis method.

Results
The results showed Pseudomonas aeruginosa isolates had the most elevated resistance to imipenem (93.3%), trimethoprim-sulfamethoxazole (93.3%), ceftriaxone (80%), ceftazidime (80%), gentamicin (60%), levofloxacin (60%), ciprofloxacin (60%), and cefepime (60%). In the broth microdilution method, 100%, 100%, 20%, and 13.3% of isolates showed resistance to imipenem, meropenem, polymyxin B, and colistin, respectively. Ten (66.6%) isolates were identified as multiple drug resistance. Carbapenemase enzymes and extended spectrum beta-lactamases were identified in 66.6% and 20% of the isolates, respectively and the biofilm formation was detected in 100% of the isolates. The blaOXA-48, blaTEM, blaIMP, blaSPM, blaPER, blaVEB, blaNDM, blaSHV, and blaCTX-M genes were detected in 100%, 86.6%, 86.6%, 40%, 20%, 20%, 13.3%, 6.6%, and 6.6% of the isolates, respectively. The blaVIM, blaGIM, blaGES, and blaMCR-1 genes were not identified in any of the isolates. The MLVA typing technique showed 11 types and seven main clusters and most isolates belong to cluster I, V and VII.

Conclusion
Due to the high rate of antimicrobial resistance, as well as the genetic diversity of Pseudomonas aeruginosa isolates from COVID-19 patients, it is indispensable to monitor the antimicrobial resistance pattern and epidemiology of the isolates on a regular basis.
Chronic heart failure health-related quality of life questionnaire (CHFQOLQ-20): development and psychometric properties
Authors: Khajavi Abdoljavad , Moshki Mahdi , Minaee Shima , Vakilian Farveh , Montazeri Ali , Hashemizadeh Haydeh 
Published: March 2023

Abstract
Background
Health-related quality of life (HRQoL) is an important outcome indicator for chronic diseases. This study aimed to develop a new instrument for assessment of HRQoL in chronic heart failure (CHF) and evaluate its psychometric properties.

Methods
This study included two steps of conceptualization and item generation, and assessment of the psychometric properties of an instrument for measuring HRQoL in patients with CHF. A sample of 495 patients with confirmed diagnosis of heart failure participated in the study. In addition to content validity, exploratory and confirmatory factor analyses, concurrent validity, convergent validity, known groups comparison were performed to assess construct validity. Internal consistency, and stability were estimated by the Cronbach’s alpha, the MacDonal’s Omega, and intraclass correlation coefficients.

Results
The content validity of the developed chronic heart failure quality of life questionnaire was assessed by 10 experts. The exploratory factor analysis indicated a four-factor solution for the instrument containing 21 items that jointly accounted for 65.65% of variance observed. The confirmatory factor analysis confirmed the four factor solution with the following fit indexes (χ2/df = 2.214, CFI = 0.947, NFI = 0.91, TLI = 0.937, IFI = 0.947, GFI = 0.899, AGFI = 0.869, RMSEA = 0.063). However, at this stage one item was removed. The concurrent and convergent validity of the CHFQOLQ-20 were established using the Short Form Health Survey (SF-36), and the MacNew Heart Disease Quality of Life Questionnaire, respectively. The known-groups validity as assessed by using the New York Heart Association (NYHA) functional classification showed that the questionnaire discriminated well between patients who differed in functional classification. The internal consistency and test-retest reliability of the CHFQOLQ-20 were satisfactory, with a Cronbach’s alpha and intraclass correlation coefficient (ICC) values of 0.93 and 0.84, respectively.

Conclusion
The results confirmed that CHFQOLQ-20 is a valid and reliable instrument for measuring quality of life (QoL) in patients with CHF. It is a short and easy-to-use instrument that is also capable of assessing the cognitive functioning, which has been overlooked in previous questionnaires.
War-related traumatic brain injuries during the Syrian armed conflict in Damascus 2014–2017: a cohort study and a literature review
Authors: Hanafi Ibrahem , Munder Eskander , Ahmad Sulafa , Arabhamo Iman , Alziab Suzan , Badin Noor , Omarain Ahmad , Jawish Mhd Khaled , Saleh Muhannad , Nickl Vera , Wipplinger Tamara , Wipplinger Christoph , Nickl Robert 
Published: March 2023

Abstract
Background
The decade-long Syrian armed conflict killed or injured more than 11% of the Syrian population. Head and neck injuries are the most frequent cause of war-related trauma, about half of which are brain injuries. Reports about Syrian brain trauma victims were published from neighboring countries; However, none are available from Syrian hospitals. This study aims to report war-related traumatic brain injuries from the Syrian capital.

Methods
We conducted a retrospective cohort study between 2014 and 2017 at Damascus Hospital, the largest public hospital in Damascus, Syria. Target patients were the victims of combat-related traumatic brain injuries who arrived alive and were admitted to the neurosurgery department or to another department but followed by the neurosurgery team. The collected data included the mechanism, type, and site of injury based on imaging findings; types of invasive interventions; intensive-care unit (ICU) admissions; as well as neurological status at admission and discharge including several severity scales.

Results
Our sample consisted of 195 patients; Ninety-six of them were male young adults, in addition to 40 females and 61 children. Injuries were caused by shrapnel in 127 (65%) cases, and by gunshots in the rest, and most of them (91%) were penetrating. Sixty-eight patients (35%) were admitted to the ICU, and 56 (29%) underwent surgery. Neurological impairment was reported in 49 patients (25%) at discharge, and the mortality rate during hospitalization was 33%. Mortality and neurological impairment associated significantly with higher values on clinical and imaging severity scores.

Conclusions
This study captured the full spectrum of war-related brain injuries of civilians and armed personnel in Syria without the delay required to transport patients to neighboring countries. Although the clinical presentation of injuries at admission was not as severe as that in previous reports, the inadequate resources (i.e., ventilators and operation rooms) and the lack of previous experience with similar injuries might have resulted in the higher mortality rate. Clinical and imaging severity scales can provide a handy tool in identifying cases with low probability of survival especially with the shortage of personal and physical resources.
Developing the modified 4-item version of perceived stress scale for functional dyspepsia
Authors: Du Limei , Yong Guizhen , Wang Ping , Wang Xi , Ming Wen , He Guobin 
Published: March 2023

Abstract
Background
To develop the modified 4-item version of Perceived Stress Scale (PSS) with a better reliability and validity than the 4-item version of PSS (PSS-4) in evaluating psychological stress in patients with functional dyspepsia (FD). The present study also aimed to explore the correlation between dyspepsia symptoms severity (DSS), anxiety, depression, somatization, quality of life (QoL), and psychological stress assessed by two approaches in FD.

Methods
A total of 389 FD patients who met the Roman IV criteria completed the 10-item version of the PSS (PSS-10), and 4/10 items were selected by five methods, such as Cronbach’s coefficient, exploratory factor analysis (EFA), correlation coefficient, discrete degree, and item analysis, to develop the modified PSS-4. The reliability and validity of the modified PSS-4 and the PSS-4 were compared by internal consistency, EFA, and confirmatory factor analysis (CFA). The correlation between psychological stress assessed by two approaches and DSS, anxiety, depression, somatization, and QoL was explored by Pearson’s correlation coefficient and multiple linear regression analysis.

Results
Cronbach’s α coefficient of the modified PSS-4 and the PSS-4 was 0.855 and 0.848, respectively, and a common factor was extracted. The cumulative contribution rate of one factor to the overall variance for the modified PSS-4 and the PSS-4 was 70.194% and 68.698%, respectively. The model used for the modified PSS-4 showed that the values of the goodness-of-fit index (GFI) and the adjusted GFI (AGFI) were 0.987 and 0.933, respectively, indicating that the model fitted well. Psychological stress was correlated to DSS, anxiety, depression, somatization, and QoL as assessed by the modified PSS-4 and PSS-4. Multiple linear regression analysis revealed that psychological stress was correlated to somatization, as assessed by the modified PSS-4 (β = 0.251, P < 0.001) and PSS-4 (β = 0.247, P < 0.001). Psychological stress, DSS, and somatization were correlated to QoL, as assessed by the modified PSS-4 (β = 0.173, P < 0.001) and the PSS-4 (β = 0.167, P < 0.001).

Conclusions
The modified PSS-4 showed better reliability and validity, and psychological stress had a greater effect on the somatization and QoL of FD patients assessed by the modified PSS-4 than PSS-4. These findings were helpful for further investigation of the clinical application of the modified PSS-4 in FD.