bluuebunny/crossref_metadata_2025_split huggingface dataset:
Accurate and Efficient 3D Reconstruction of Right Heart Shape and Motion from Multi-Series Cine-MRI
Authors: Renzi Francesca , Vergara Christian , Fedele Marco , Giambruno Vincenzo , Quarteroni Alfio Maria , Puppini Giovanni , Luciani Giovanni Battista 
Published: June 2023

AbstractThe accurate reconstruction of the right heart geometry and motion from time-resolved medical images enhances diagnostic tools based on image visualization as well as the analysis of cardiac blood dynamics through computational methods. Due to the peculiarity of the right heart morphology and motion, commonly used segmentation and/or reconstruction techniques, which only employ Short-Axis cine-MRI, lack accuracy in relevant regions of the right heart, like the ventricular base and the outflow tract. Moreover, the reconstruction procedure is time-consuming and, in the case of the generation of computational domains, requires a lot of manual intervention.This paper presents a new method for the accurate and efficient reconstruction of the right heart geometry and motion from time-resolved MRI. In particular, the proposed method makes use of surface morphing to merge information coming from multi-series cine-MRI (such as Short/Long-Axis and 2/3/4 Chambers acquisitions) and to reconstruct important cardiac features. It also automatically provides the complete cardiac contraction and relaxation motion by exploiting a suitable image registration technique. The method is applied both to a healthy and a pathological (tetralogy of Fallot) case, and yelds more accurate results than standard procedures. The proposed method is also employed to provide significant input for computational fluid dynamics. The corresponding numerical results demonstrate the reliability of our approach in the computation of clinically relevant blood dynamics quantities.
Differential Inhibition of Intra- and Inter-molecular Protease Cleavages by Antiviral Compounds
Authors: Doherty Jennifer S. , Kirkegaard Karla 
Published: June 2023

ABSTRACTEnteroviruses encode two protease active sites, in the 2A and 3C coding regions. While they target many host proteins, they first need to be excised from the viral polyprotein in which they are embedded. Polyprotein cleavage can occur either intra-molecularly (incis) or inter-molecularly (intrans). Previous work suggested that antivirals targeting intra-molecular cleavages could generate inhibitory precursors that suppress the outgrowth of drug-resistant variants. Therefore, we wanted to evaluate enteroviral cleavage patterns to identify such obligate intra-molecular cleavages for drug target selection. Using translation extracts, we show thatciscleavage of the 2A protease N-terminal junction is conserved across three enteroviruses, while the mechanism for the N-terminal junction of 3C varies, with EV-D68 3C cleavage occurring incisand poliovirus 3C cleavage occurring intrans.Antiviral agents targeting proteases are often identified via their ability to block the cleavage of artificial peptide substrates. Here, we show that antivirals identified for their abilities to block inter-molecular cleavage can sometimes block intra-molecular cleavage of the protease from its polyprotein as well, but with widely varying efficacy. Additionally, we demonstrate that, for three enteroviral species, genomes defective in 2A protease activity suppress the growth of wild-type virus in mixed populations, supporting the hypothesis that preventing intra-molecular cleavage at the VP1·2A junction can create dominantly inhibitory precursors. These data argue that, to reduce the likelihood of drug resistance, protease-targeted antivirals should be evaluated for their ability to block intra-molecular polyprotein cleavages in addition to inter-molecular cleavage of other substrates.IMPORTANCEMost protease-targeted antiviral development evaluates the ability of small molecules to inhibit cleavage of model substrates. However, before they can cleave any other substrates, viral proteases need to cleave themselves from the viral polyprotein in which they have been translated. This can occur either intra- or inter-molecularly. Here, we show that, for poliovirus, Enterovirus D68 and Enterovirus A71, many of these cleavages are required to occur intra-molecularly. Further, we show that antivirals identified for their ability to block cleavage of artificial substrates can also block intra-molecular self-cleavage, but that their efficacy in doing so varies widely. We argue that evaluating candidate antivirals for their ability to block these cleavages is vital to drug development, because the buildup of uncleaved precursors can be inhibitory to the virus and potentially suppress the selection of drug-resistant variants.
Normal gastric tissueHelicobacter pyloriinfection is associated with epigenetic age acceleration, increased mitotic tick rate, tissue cell composition, and Natural Killer cell methylation alterations
Authors: Vlasac Irma M. , Christensen Brock C. , Salas Lucas A. 
Published: June 2023

AbstractBackgroundGastric adenocarcinomas are a leading cause of global mortality, associated with chronic infection withHelicobacter pylori. The mechanisms by which infection withH. pyloricontributes to carcinogenesis are not well understood. Recent studies from subjects with and without gastric cancer have identified significant DNA methylation alterations in normal gastric mucosa associated withH. pyloriinfection and gastric cancer risk. Here we further investigated DNA methylation alterations in normal gastric mucosa in gastric cancer cases (n = 42) and control subjects (n = 42) withH. pyloriinfection data. We assessed tissue cell type composition, DNA methylation alterations within cell populations, epigenetic aging, and repetitive element methylation.ResultsIn normal gastric mucosa of both gastric cancer cases and control subjects, we observed increased epigenetic age acceleration associated withH. pyloriinfection. We also observed an increased mitotic tick rate associated withH. pyloriinfection in both gastric cancer cases and controls. Significant differences in immune cell populations associated withH. pyloriinfection in normal tissue from cancer cases and controls were identified using DNA methylation cell type deconvolution. We also found natural killer cell-specific methylation alterations in normal mucosa from gastric cancer patients withH. pyloriinfection.ConclusionsOur findings from normal gastric mucosa provide insight into underlying cellular composition and epigenetic aspects ofH. pyloriassociated gastric cancer etiology.
Loss of Tumour Suppressor TMEM127 Drives RET-mediated Transformation Through Disrupted Membrane Dynamics
Authors: Walker Timothy J. , Reyes-Alvarez Eduardo , Hyndman Brandy D. , Sugiyama Michael G. , Oliveira Larissa C.B. , Rekab Aisha N. , Crupi Mathieu J.F. , Cabral-Dias Rebecca , Guo Qianjin , Dahia Patricia L.M. , Richardson Douglas S. , Antonescu Costin N. , Mulligan Lois M. 
Published: June 2023

AbstractInternalization from the cell membrane and endosomal trafficking of receptor tyrosine kinases (RTK) are important regulators of signaling in normal cells that can frequently be disrupted in cancer. The adrenal tumour pheochromocytoma (PCC) can be caused by activating mutations of the RET receptor tyrosine kinase, or inactivation of TMEM127, a transmembrane tumour suppressor implicated in trafficking of endosomal cargos. However, the role of aberrant receptor trafficking in PCC is not well understood. Here, we show that loss of TMEM127 causes wildtype RET protein accumulation on the cell surface, where increased receptor density facilitates constitutive ligand-independent activity and downstream signaling, driving cell proliferation. Loss of TMEM127 altered normal cell membrane organization and recruitment and stabilization of membrane protein complexes, impaired assembly, and maturation of clathrin coated pits, and reduced internalization and degradation of cell surface RET. In addition to RTKs, TMEM127 depletion also promoted surface accumulation of several other transmembrane proteins, suggesting it may cause global defects in surface protein activity and function. Together, our data identify TMEM127 as an important determinant of membrane organization including membrane protein diffusability, and protein complex assembly and provide a novel paradigm for oncogenesis in PCC where altered membrane dynamics promotes cell surface accumulation and constitutive activity of growth factor receptors to drive aberrant signaling and promote transformation.
Automated data extraction of unstructured grey literature in health research: a mapping review of the current research literature
Authors: Schmidt Lena , Mohamed Saleh , Meader Nick , Bacardit Jaume , Craig Dawn 
Published: June 2023

AbstractThe amount of grey literature and ‘softer’ intelligence from social media or websites is vast. Given the long lead-times of producing high-quality peer-reviewed health information this is causing a demand for new ways to provide prompt input for secondary research. To our knowledge this is the first review of automated data extraction methods or tools for health-related grey literature and soft intelligence, with a focus on (semi)automating horizon scans, health technology assessments, evidence maps, or other literature reviews.We searched six databases to cover both health– and computer-science literature. After deduplication, 10% of the search results were screened by two reviewers, the remainder was single-screened up to an estimated 95% sensitivity; screening was stopped early after screening an additional 1000 results with no new includes. All full texts were retrieved, screened, and extracted by a single reviewer and 10% were checked in duplicate.We included 84 papers covering automation for health-related social media, internet fora, news, patents, government agencies and charities, or trial registers. From each paper we answered three research questions: Firstly, important functionalities for users of the tool or method; secondly, information about the level of support and reliability; and thirdly, practical challenges and research gaps.Poor availability of code, data, and usable tools leads to low transparency regarding performance and duplication of work. Financial implications, scalability, integration into downstream workflows, and meaningful evaluations should be carefully planned before starting to develop a tool, given the vast amounts of data and opportunities those tools offer to expedite research.
Arylamine N-acetyltransferase-1 reveals a subpopulation of ALS patients with altered metabolic capacity
Authors: Choudhury Chandra , Allen Sally , Gill Melinder K. , Garton Fleur , Restuardi Restuadi , Butcher Neville J. , Ngo Shyuan T. , Steyn Frederik J. , Minchin Rodney F. 
Published: June 2023

AbstractAmyotrophic lateral sclerosis (ALS) is a heterogeneous disease characterised by metabolic changes at onset and throughout disease progression. Here, we investigate the role of arylamine N-acetyltransferase 1 (NAT1), a cytosolic protein associated with mitochondrial function, in ALS. We demonstrate that expression of the murine homolog (mNat2) increases in skeletal muscle of SODG93Amice, but not control animals, at onset of symptoms and remains elevated until end stage of the disease. Measurement of mitochondrial respiration in peripheral blood mononuclear cells of patients with ALS identified patient sub-populations with low and high metabolic potential, which was strongly associated with NAT1 activity. Those patients with high NAT1 activity had elevated basal respiration, ATP production, mitochondrial reserve, and aerobic glycolysis. NAT1 predicted increased whole body metabolic index, which may be clinically significant as these patients show increased functional decline and shorter survival. NAT1 may be a novel target in those patients with elevated activity.
Task-specific invariant representation in auditory cortex
Authors: Heller Charles R. , Hamersky Gregory R. , David Stephen V. 
Published: June 2023

AbstractCategorical sensory representations are critical for many behaviors, including speech perception. In the auditory system, categorical information is thought to arise hierarchically, becoming increasingly prominent in higher order cortical regions. The neural mechanisms that support this robust and flexible computation remain poorly understood. Here, we studied sound representations in primary and non-primary auditory cortex while animals engaged in a challenging sound discrimination task. Population-level decoding of simultaneously recorded single neurons revealed that task engagement caused categorical sound representations to emerge in non-primary auditory cortex. In primary auditory cortex, task engagement caused a general enhancement of sound decoding that was not specific to task-relevant categories. These findings are consistent with mixed selectivity models of neural disentanglement, in which early sensory regions build an overcomplete representation of the world and allow neurons in downstream brain regions to flexibly and selectively read out behaviorally relevant, categorical information.
Molecular Signatures of Attention Networks
Authors: Schindler Hanna , Jawinski Philippe , Arnatkevičiūtė Aurina , Markett Sebastian 
Published: June 2023

AbstractAttention network theory proposes three distinct types of attention - alerting, orienting, and control - that are supported by separate brain networks and modulated by different neurotransmitters, i.e., noradrenaline, acetylcholine, and dopamine. Here, we explore the extent of cortical, genetic, and molecular dissociation of these three attention systems using multimodal neuroimaging. We evaluated the spatial overlap between fMRI activation maps from the attention network test (ANT) and cortex-wide gene expression data from the Allen Human Brain Atlas. The goal was to identify genes associated with each of the attention networks in order to determine whether specific groups of genes were co-expressed with the corresponding attention networks. Furthermore, we analysed publicly available PET-maps of neurotransmitter receptors and transporters to investigate their spatial overlap with the attention networks.Our analyses revealed a substantial number of genes (3871 for alerting, 6905 for orienting, 2556 for control) whose cortex-wide expression co-varied with the activation maps, prioritizing several molecular functions such as the regulation of protein biosynthesis, phosphorylation, and receptor binding. Contrary to the hypothesized associations, the ANT activation maps neither aligned with the distribution of noradrenaline, acetylcholine, and dopamine receptor and transporter molecules, nor with transcriptomic profiles that would suggest clearly separable networks. Independence of the attention networks appeared additionally constrained by a high level of spatial dependency between the network maps. Future work may need to re-conceptualize the attention networks in terms of their segregation and re-evaluate the presumed independence at the neural and neurochemical level.
The relationship among relationship-selling, celebrity attachment and customer engagement
Authors: Yeh Ching-Wen 
Published: June 2023

PurposeThis study explores the relationship among relationship-selling, celebrity attachment and customer engagement.Design/methodology/approachHypotheses were tested, using structural equation modeling on survey responses of 321 participants.FindingsThis study determines the connection between relationship-selling factors and customer engagement using the stimulus-organism-response (SOR) theory. Results demonstrate that interactional intensity, mutual disclosure and cooperative intention exert considerable positive effects on celebrity attachment, which, in turn, significantly impacts customer engagement.Originality/valueThe research findings add to the existing body of knowledge through more information on the degree to which relationship-selling factors affect celebrity attachment, and eventually, customer engagement. The study also aims to prompt researchers and organizations to consider effective communication strategies to increase online customer engagement.
Entrepreneurial orientation, proactive market orientation and society: evidence from public service organizations in Brazil
Authors: Hodgkinson Ian R. , Hughes Paul , Leite Higor , Lee Younggeun 
Published: June 2023

PurposePublic service organizations (PSOs) face a critical dilemma: how to generate more value for society but with a much-reduced resource base. The article advances the strategy axis of entrepreneurial orientation (EO) research by examining EO and proactive market orientation (PMO) as joint-strategic approaches to this end, and how the characteristics of public managers may moderate the paths to value creation.Design/methodology/approachThe article draws on a unique survey-based dataset developed from Brazilian PSOs and employs structural equation modelling for hypotheses testing. Post-hoc analysis, by way of analysis of variance, demonstrates the joint impact of the two strategic approaches on public service performance level.FindingsEntrepreneurial and PMOs are revealed as routes to enhanced service performance, but managers’ domain expertise negatively moderates these relationships. Post-hoc analysis reveals how organizations displaying higher levels of both orientations realize superior performance, relative to those favouring either/or.Originality/valueThe study contributes new evidence for EO model specificity by examining a narrowly bounded sample of PSOs; addresses the neglect of other outcome variables beyond traditional performance, showing the value of EO for society and offers new insights to the managerial conditions that moderate the positive synergies between EO, PMO and service performance.
Examining the relationship between negative social exchange and suicidal ideation: the role of thwarted interpersonal needs
Authors: Hill Ryan M. , Pardue-Bourgeois Sarah , Perkins Kaylee Myichell , Hussain Zain 
Published: June 2023

Purpose
Suicidal ideation (SI) is a serious public health concern among youth and young adults in the USA. It is critical to evaluate potential risk and protective factors associated with SI among young adults to develop prevention strategies that target novel mechanistic outcomes.

Design/methodology/approach
This study sought to evaluate the association between negative social exchange (NSE) and SI via the framework of the interpersonal theory of suicide. Participants were a diverse sample of N = 243 college students, with a mean age of 19.98 years (SD = 3.15). Participants were predominantly female (68.3%) and Hispanic/Latinx (39.5%), white (21.4%) or multiracial/multiethnic (24.3%). Participants completed survey measures to assess each of the constructs of interest.

Findings
Results indicated a significant indirect effect from NSE to SI via perceived burdensomeness, but not thwarted belongingness.

Originality/value
While individual-level interventions target emotional reactivity and managing emotional responses, larger public health–focused interventions on campus may wish to target reductions in NSE on campus. Future work should evaluate these associations using longitudinal study designs to further evaluate the statistical models.
Link between job burnout antecedents and consequences: an empirical study on higher education faculty members in the Saudi context
Authors: Asfahani Ahmed M. 
Published: June 2023

PurposeThis study aimed to examine the antecedents, correlates, and consequences of burnout among higher education faculty in Saudi Arabia using the theoretical framework of the job demands-resources model.Design/methodology/approachUsing a quantitative research design, a cross-sectional survey was employed to collect data from faculty members across multiple Saudi universities. The constructs were measured using validated scales, and data analysis included exploratory factor analysis, Pearson correlation analysis, factorial ANOVA, and multiple regression.FindingsThe study identified moderate levels of burnout, confirming a significant positive relationship with role conflict and a negative relationship with internal locus of control. Burnout significantly contributed to depression, insomnia, and turnover intentions. However, no significant relationship was found between burnout and workplace conflict when controlling for other variables.Research limitations/implicationsThe study's findings can inform policymakers and academic administrators about measures to alleviate faculty burnout, thus contributing to healthier academic work environments aligned with Saudi Arabia's Vision 2030 goals.Originality/valueThis research extends the job demands-resources model within the context of higher education institutions in Saudi Arabia, offering nuanced insights into burnout dynamics among university faculty in this region. Despite the model's robustness, the absence of a significant relationship between burnout and workplace conflict signals the need for a more intricate understanding of burnout's antecedents and consequences.
The influence of social network communication on the buying behavior of Cameroonian consumers on social e-commerce platforms
Authors: Kala Kamdjoug Jean Robert 
Published: June 2023

PurposeThe paper explores how social networks influence Cameroonian consumers' buying behavior. Then, the authors examine customers' advertising perceptions and psychological dispositions to explain their purchase intention and behavioral consumption.Design/methodology/approachThe research framework is developed based on Nelson's theory of advertising by studying advertising perceptions, consumer psychological dispositions associated with social network characteristics and behavioral consumption. Using partial least squares structural equation modeling (PLS-SEM), the validation takes support from 231 responses collected with an online questionnaire from Cameroun.FindingsThe study reveals three critical results: (1) consumers' perceptions of advertising significantly influence their psychological disposition, (2) consumers' psychological dispositions and the social network significantly influence their intention to purchase and (3) consumers' intention to purchase significantly impacts their behavioral consumption.Originality/valueThe proposed and validated model contributes to understanding the influence of social network communication on customers' buying behavior on social s-Commerce platforms of developing country enterprises.
Prices, uses, and (r)evolutionary change: the outlook for post-pandemic office investing
Authors: Fagan Kevin , Li Xiaodi , Jabir Ermengarde , Calanog Victor 
Published: June 2023

PurposeThe authors take a historical perspective and compare office market performance metrics and CMBS loan delinquency rates over the past two years with previous downturns.Design/methodology/approachWhat will happen to the office sector in the post-pandemic era? we examine this question from three perspectives. First, the authors discuss the (short-term) risk of commercial real estate investment with high inflation and rising interest rates. If investors want to use CRE as an inflationary hedge, the cash flow must increase enough to counteract growing cap rates given rising interest rates.FindingsAs it turns out, the COVID-19 recession has been notably innocuous. Third, the authors focus on medical office space – an emerging investment option for the office sector.Practical implicationsThe authors remain somewhat positive (or at least less downbeat) about the future of the office market based on the data they reviewed.Originality/valueThe office market is experiencing an odyssey rather than an exodus, at least in the short run. However, the authors remain cautious and they are monitoring key signs, prepared for the possibility of (r)evolutionary change in the office sector.