bluuebunny/crossref_metadata_2025_split huggingface dataset:
Faktor Risiko Kontaminasi Salmonella Spp. Pada Daging Ayam Broiler (Gallus Gallus Domestica) Yang Dijual di Pasar Banguntapan
Authors: Ubaidillah Ubaidillah , Haryati Handa 
Published: July 2023

Background of Study: Chicken is a food ingredient with high nutritional value that is consumed by community, the high nutritional value of chicken meat makes it a food ingredient that is susceptible to biological hazards. One of the microbes that contaminate chicken meat is Salmonella Spp. These bacteria cause disease by food borne transmission and are mostly pathogenic. This pollution is caused by the environment, handling and marketing errors. The purpose of this study is to determine the risk factors for contamination of Salmonella Spp. on Broiler Chicken sold at Banguntapan MarketMethods: This research method was quantitative research with a cross sectional approach. The population of all chicken traders in the Banguntapan market. The sampling technique used purposive sampling technique, and the data were analyzed using the Chi Square Test.Results: The results of data analysis, there was a significant influence between environmental factors on Salmonella Spp contamination on Broiler Chicken with a p value of (0.033 0.05). There was a significant influence between Hygiene factors and seller activity on Salmonella Spp contamination on broiler meat with a p value of (0.013 0.05). There was a significant effect between the pH factor on Salmonella Spp contamination on broiler chicken with a p value of (0.0100.05). There is no significant effect of temperature on Salmonella Spp contamination on Broiler Chicken with a p value of (0.3510.05)Conclusions: Risk Factor in this research, there was a significant influence between the selling environment, hygiene and seller activities, and pH on the risk of Salmonella Spp contamination in Broiler Chicken (Gallus gallus domestica), and there was no significant effect between temperature and contamination of Salmonella Spp. on Broiler Chicken (Gallus gallus domestica) at Banguntapan MarketKeywords: Salmonella Spp; broiler chicken; Gallus gallus domesticus
Tafsir Hakim Judex Facti dan Judex Juris Terkait Batas Maksimal Upaya Administratif
Authors: Said Muhtar , Erfandi Erfandi 
Published: July 2023

Untuk mengajukan upaya administratif berupa keberatan diberikan waktu maksimal 21 hari kerja. Meskipun demikian ada perbedaan penerapan terhadap upaya administratif tersebut antara hakim judex facti dengan hakim judex juris. Hakim judex facti melalui putusan nomor 21/G/2020/PTUN dan 76/B/2021/PT.TUN JKT menerapkan upaya administratif adalah hal yang wajib diterapkan, putusan ini dikuatkan oleh hakim di Pengadilan Tingkat Tinggi Tata Usaha Negara. Namun putusan tersebut dibatalkan oleh hakim judex juris (tingkat Mahkamah Agung) melalui putusan nomor 420 K/TUN/2021. Dasar judex juris melakukan pembatalan terhadap putusan judex facti didasarkan pada frasa “dapat” yang tercantum pada Pasal 75 ayat (1) UU Nomor 30 tahun 2014 tentang Administrasi Pemerintahan. Sehingga upaya administratif melalui keberatan maupun banding administratif merupakan suatu pilihan bukan wajib. Upaya administratif merupakan usaha untuk mewujudkan prinsip Pancasila ke dalam penyelesaian permasalahan administrasi, yakni dalam penyelesaian permasalahan administrasi supaya terlebih dahulu diselesaikan melalui musyawarah mufakat. Apabila musyawarah mufakat tidak bisa menemukan titik perdamaian maka bisa diselesaikan melalui Pengadilan Tata Usaha Negara.
Pemberian Terapi Spiritual Emotional Freedom Technique (SEFT) bagi Pasien Terkonfirmasi Covid-19
Authors: Rantesigi Nirva , Agusrianto Agusrianto , Supriadi Supriadi , Sufyaningsih Ulfa 
Published: July 2023

The covid-19 pandemic can have an impact on both physical and psychological problems for every individual, especially those who have to experience isolation/ quarantine. The forms of distress that arise due to COVID-19 inclide: sleep problems, fear of viruses, difficulty focusing, anxiety, and depression. Spiritual Emotional Freedom Technique (SEFT) is one technique that has been proven effective in dealing with anxiety and increasing motivation. The purpose of this activity is to increase knowledge, reduce anxiety, and reduce stress for confirmed Covid-19 patients. This community service was carried out in the Gebang Rejo Village by using video as a medium.This education related of the impact or danger of anxiety was attended by 24 participants, consisting of 20 women and 4 men. The community was very enthustiastic in listening to the educational materials. After providing education, then continued with the provision of SEFT therapy. From the results of the evaluation of the level of anxiety after community service, it was found that there was a decrease in the number of severe anxiety from 20,8% to 2,4%, moderate anxiety from 37,5% to 20,8% and an increase in mild anxiety from 41,6% to 75%. The conclusion is that giving SEFT therapy can reduce anxiety levels inconvirmed covid-19 patients.
Treating subclinical hypothyroidism in individuals with or without mental health problems –A Delphi based expert consensus study in two countries
Authors: Lieber Ingrid , Van Der Feltz-Cornelis Christina Maria , Razvi Salman , Moriarty Andrew S. , Wilkes Scott , Ott Michael , Mannchen Julie , Eliasson Mats , Werneke Ursula 
Published: July 2023

BackgroundSubclinical hypothyroidism (SCH) is a common endocrine problem with prevalence estimates between 4% and 20%. Symptoms are often non-specific but can substantially affect well-being leading to repeated medical consultations. The effect of thyroid hormone replacement therapy (THRT) in patients with SCH remains uncertain. Current guidelines, limited by the lack of high-quality evidence, have been controversial with limited adherence in clinical practice.MethodsThree-round modified Delphi method to establish consensus regarding diagnosis and treatment of individuals with SCH with and without affective disorder or anxiety, conducted with clinicians from three specialties, general practice, endocrinology and psychiatry, and two countries, Sweden and the United Kingdom.ResultsSixty clinicians, 20 per specialty, were recruited. Fifty-three (88%) participants completed all three rounds. The participants reached consensus on five of the 26 practice statements that (a) repeated testing was required for the diagnosis of subclinical hypothyroidism, (b) antibody screening should usually occur, and (c and d) antibody screening would strengthen the indication for thyroid hormone replacement therapy in both individuals with or without affective disorder or anxiety. The participants disagreed with (e) a requirement of a TSH threshold ≥ 20 mIU/L for thyroid hormone replacement therapy start. Psychiatrists and GPs but not endocrinologists, agreed that there was a frequent discrepancy between laboratory results and clinical symptoms, and disagreed that testing for thyroid dysfunction was overused in patients presenting with depression or anxiety, or fatigue.ConclusionsIn many aspects, attitudes toward diagnosing and treating SCH remain diverse. The inability of our Delphi panel to achieve consensus on most items and the disagreement with a TSH ≥ 20 mIU/L threshold for treatment suggest that the concept of SCH may need rethinking with a better understanding of the hypothalamic-pituitary-thyroid physiology. Given that the scientific evidence is currently not conclusive, guidelines in this area should not be taken as definitive.
Single-cell RNA sequencing reveals tumor immune microenvironment in human hypopharygeal squamous cell carcinoma and lymphatic metastasis
Authors: Li Ce , Guan Rui , Li Wenming , Wei Dongmin , Cao Shengda , Xu Chenyang , Chang Fen , Wang Pin , Chen Long , Lei Dapeng 
Published: July 2023

BackgroundHuman hypopharygeal squamous cell carcinoma (HSCC) is a common head and neck cancer with a poor prognosis in advanced stages. The occurrence and development of tumor is the result of mutual influence and co-evolution between tumor cells and tumor microenvironment (TME). Tumor immune microenvironment (TIME) refers to the immune microenvironment surrounding tumor cells. Studying TIME in HSCC could provide new targets and therapeutic strategies for HSCC.MethodsWe performed single-cell RNA sequencing (scRNA-seq) and analysis of hypopharyngeal carcinoma, paracancerous, and lymphoid tissues from five HSCC patients. Subdivide of B cells, T cells, macrophages cells, and monocytes and their distribution in three kinds of tissues as well as marker genes were analyzed. Different genes of IGHG1 plasma cells and SPP1+ macrophages between HSCC tissues, adjacent normal tissues and lymphatic tissues were analyzed. Additionally, we studied proliferating lymphocytes, T cells exhaustion, and T cell receptor (TCR) repertoire in three kinds of tissues.ResultsTranscriptome profiles of 132,869 single cells were obtained and grouped into seven cell clusters, including epithelial cells, lymphocytes, mononuclear phagocytics system (MPs), fibroblasts, endothelial cells (ECs), plasmacytoid dendritic cells (pDCs), and mast cells. Tumor metastasis occurred in three lymphoid tissues. Four distinct populations were identified from lymphocytes, including B cells, plasma cells, T cells and proliferating lymphocytes. We found IGHA1 and IGHG1 specific plasma cells significantly overexpressed in HSCC tissues compared with normal hypopharygeal tissues and lymphatic tissues. Five distinct populations from MPs were identified, including macrophages, monocytes, mature dendritic cells (DCs), Type 1 conventional dendritic cells (cDC1) and Type 2 conventional dendritic cells (cDC2). SPP1+ macrophages were significantly overexpressed in HSCC tissues and lymphatic tissues compared with normal hypopharygeal tissues, which are thought to be M2-type macrophages. Exhaustion of CD8+ Teff cells occurred in HSCC tissues. At last, we verified that IgA and IgG1 protein expression levels were significantly up-regulated in HSCC tissues compared to adjacent normal tissues.ConclusionOverall, this study revealed TIME in HSCC and lymphatic metastasis, and provided potential therapeutic targets for HSCC.
Assessing the causal relationship between genetically determined inflammatory biomarkers and low back pain risk: a bidirectional two-sample Mendelian randomization study
Authors: Li Wenhan , Lu Qunwen , Qian Junhui , Feng Yue , Luo Jian , Luo Caigui , He Wenshan , Dong Bing , Liu Huahui , Liu Zhongxing , Su Chengguo 
Published: July 2023

BackgroundObservational studies have suggested an association between inflammatory markers and low back pain (LBP), but the causal relationship between these factors remains uncertain.MethodsWe conducted a bidirectional two-sample Mendelian randomization analysis (MR) study to investigate whether there is a causal relationship between inflammatory markers and low back pain. We obtained genetic data for CRP, along with its upstream inflammatory markers IL-6, IL-8, and IL-10, as well as low back pain from publicly available genome-wide association studies (GWAS). We applied several MR methods, including inverse variance weighting, weighted median, MR-Egger, Wald Ratio, and MR-PRESSO, to test for causal relationships. Sensitivity analyses were also conducted to assess the robustness of the results.ResultsOur analyses utilizing the Inverse Variance Weighted (IVW) method, the MR-Egger method, and the weighted median method indicated that IL-6 may be associated with an increased risk of LBP (Effect Size: -0.009, 95% Confidence Interval: -0.013–0.006, p = 9.16e-08); however, in the reverse direction, there was no significant causal effect of LBP on inflammatory markers.ConclusionOur study used a Mendelian randomization approach and found that elevated IL-6 levels may reduce the risk of LBP.
Screening, molecular identification, population diversity, and antibiotic susceptibility pattern of Actinomycetes species isolated from meat and meat products of slaughterhouses, restaurants, and meat stores of a developing country, Iran
Authors: Motallebirad Tahereh , Mardanshah Omid , Safarabadi Mehdi , Ghaffari Kazem , Orouji Mohammad Ali , Abedi Behnam , Azadi Davood 
Published: July 2023

IntroductionActinomycetes can colonize surfaces of tools and equipment and can be transferred to meat and meat products during manufacture, processing, handling, and storage. Moreover, washing the meat does not eliminate the microorganisms; it only spreads them. As a result, these opportunistic pathogens can enter the human body and cause various infections. Therefore, the aim of the current study was to screen, identify, and determine the antibiotic susceptibility of Actinomycetes species from meat and meat products in the Markazi province of Iran.MethodsA total of 60 meat and meat product samples, including minced meat, mutton, beef, chicken, hamburgers, and sausages, were collected from slaughterhouses, butchers, and restaurants in the Markazi province of Iran. The samples were analyzed using standard microbiological protocols for the isolation and characterization of Actinomycetes. PCR amplification of hsp65 and 16SrRNA genes and sequence analysis of 16SrRNA were used for genus and species identification. The minimum inhibitory concentrations (MICs) of antimicrobial agents were determined by the broth microdilution method and interpreted according to the CLSI guidelines.ResultsA total of 21 (35%) Actinomycetes isolates from 5 genera and 12 species were isolated from 60 samples. The most prevalent Actinomycetes were from the genus Mycobacterium, with six (28.6%) isolates (M. avium complex, M. terrae, M. smegmatis, and M. novocastrense), followed by the genus Rhodococcus with five (23.8%) isolates (R. equi and R. erythropolis), the genus Actinomyces with four (19.1%) isolates (A. ruminicola and A. viscosus), the genus Nocardia with four (19.1%) isolates (N. asiatica, N. seriolae, and N. niigatensis), and the genus Streptomyces with two (9.5%) isolates (S. albus). Chicken and sausage samples had the highest and lowest levels of contamination, with six and one isolates. Respectively, the results of drug susceptibility testing (DST) showed that all isolates were susceptible to Ofloxacin, Amikacin, Ciprofloxacin, and Levofloxacin, whereas all of them were resistant to Doxycycline and Rifampicin.DiscussionThe findings suggest that meat and meat products play an important role as a reservoir for the transmission of Actinomycetes to humans, thus causing life-threatening foodborne diseases such as gastrointestinal and cutaneous disorders. Therefore, it is essential to incorporate basic hygiene measures into the cycle of meat production to ensure food safety.
Assessing network-based methods in the context of system toxicology
Authors: Valls-Margarit Jordi , Piñero Janet , Füzi Barbara , Cerisier Natacha , Taboureau Olivier , Furlong Laura I. 
Published: July 2023

Introduction: Network-based methods are promising approaches in systems toxicology because they can be used to predict the effects of drugs and chemicals on health, to elucidate the mode of action of compounds, and to identify biomarkers of toxicity. Over the years, the network biology community has developed a wide range of methods, and users are faced with the task of choosing the most appropriate method for their own application. Furthermore, the advantages and limitations of each method are difficult to determine without a proper standard and comparative evaluation of their performance. This study aims to evaluate different network-based methods that can be used to gain biological insight into the mechanisms of drug toxicity, using valproic acid (VPA)-induced liver steatosis as a benchmark.Methods: We provide a comprehensive analysis of the results produced by each method and highlight the fact that the experimental design (how the method is applied) is relevant in addition to the method specifications. We also contribute with a systematic methodology to analyse the results of the methods individually and in a comparative manner.Results: Our results show that the evaluated tools differ in their performance against the benchmark and in their ability to provide novel insights into the mechanism of adverse effects of the drug. We also suggest that aggregation of the results provided by different methods provides a more confident set of candidate genes and processes to further the knowledge of the drug’s mechanism of action.Discussion: By providing a detailed and systematic analysis of the results of different network-based tools, we aim to assist users in making informed decisions about the most appropriate method for systems toxicology applications.
Effect of COVID-19 pandemic on serious mental illness-related outpatient department utilization in Ningbo, China: an interrupted time series analysis
Authors: Li Lian , Yang Hongying , Zhang Rongxiang , Wang Yucheng , Bian Guolin 
Published: July 2023

BackgroundGlobally, the coronavirus disease 2019 (COVID-19) pandemic has negatively affected mental health services, but there is no clear evidence of this in China. Therefore, we examined the effect of the COVID-19 pandemic on the use of serious mental illness (SMI)-related outpatient services in Ningbo, China.MethodsWe analyzed the trends in monthly SMI-related outpatient department utilization from January 2018 to June 2022 using interrupted time series (ITS) regression analysis, and we defined the onset of the COVID-19 pandemic as January 2020. We also performed ITS regression analyses for sex and age subgroups.ResultsA significant difference in the monthly number of outpatient visit slopes before and after the onset of the pandemic was shown in the SMI analysis [−175.6, 95% confidence interval (CI) (−338.3 to −12.9), p &lt; 0.05]. All sex and age categories, except the 20–30 years age category, showed statistically significant changes in their slopes after the onset of the pandemic. Significant differences in the number of outpatient visit slopes before and after the onset of the pandemic were seen for schizophrenia and bipolar disorders [−153.3, 95% CI (−294.1 to −12.5) and −16.8 (−31.0 to −2.6), respectively]. Moreover, a negative relationship was observed between the monthly number of outpatient visits and the number of incidents and accidents due to SMI (r = −0.38, p &lt; 0.05).ConclusionThe COVID-19 pandemic has had a negative effect on SMI-related outpatient visits in Ningbo, especially by patients with schizophrenia. A strategy should be developed and implemented to maintain access to SMI services during the COVID-19 pandemic.
Diabetes websites lack information on dietary causes, risk factors, and preventions for type 2 diabetes
Authors: Crummett Lisa T. , Aslam Muhammad H. 
Published: July 2023

IntroductionType 2 diabetes (T2D) is a growing public health burden throughout the world. Many people looking for information on how to prevent T2D will search on diabetes websites. Multiple dietary factors have a significant association with T2D risk, such as high intake of added sugars, refined carbohydrates, saturated fat, and red meat or processed meat; and decreased intake of dietary fiber, and fruits/vegetables. Despite this dietary information being available in the scientific literature, it is unclear whether this information is available in gray literature (websites).ObjectiveIn this study, we evaluate the use of specific terms from diabetes websites that are significantly associated with causes/risk factors and preventions for T2D from three term categories: (A) dietary factors, (B) nondietary nongenetic (lifestyle-associated) factors, and (C) genetic (non-modifiable) factors. We also evaluate the effect of website type (business, government, nonprofit) on term usage among websites.MethodsWe used web scraping and coding tools to quantify the use of specific terms from 73 diabetes websites. To determine the effect of term category and website type on the usage of specific terms among 73 websites, a repeated measures general linear model was performed.ResultsWe found that dietary risk factors that are significantly associated with T2D (e.g., sugar, processed carbohydrates, dietary fat, fruits/vegetables, fiber, processed meat/red meat) were mentioned in significantly fewer websites than either nondietary nongenetic factors (e.g., obesity, physical activity, dyslipidemia, blood pressure) or genetic factors (age, family history, ethnicity). Among websites that provided “eat healthy” guidance, one third provided zero dietary factors associated with type 2 diabetes, and only 30% provided more than two specific dietary factors associates with type 2 diabetes. We also observed that mean percent usage of all terms associated with T2D causes/risk factors and preventions was significantly lower among government websites compared to business websites and nonprofit websites.ConclusionDiabetes websites need to increase their usage of dietary factors when discussing causes/risk factors and preventions for T2D; as dietary factors are modifiable and strongly associated with all nondietary nongenetic risk factors, in addition to T2D risk.
Knowledge and attitude factors associated with the prevalence of Tdap (tetanus, diphtheria, and acellular pertussis) booster vaccination in healthcare workers in a large academic hospital in Southern Italy in 2022: a cross-sectional study
Authors: Mercogliano Michelangelo , Fiorilla Claudio , Esposito Federica , Sorrentino Michele , Mirizzi Pasquale Domenico , Parisi Antonio , Tajani Andrea , Buonocore Gaetano , Triassi Maria , Palladino Raffaele 
Published: July 2023

IntroductionIn Europe, there is still suboptimal tetanus, diphtheria, and acellular pertussis (Tdap) booster coverage. This study aimed to assess coverage status, knowledge, and attitude on Tdap vaccination in healthcare workers (HcWs) of the University Hospital “Federico II” in Naples, Southern Italy, in 2022, to improve current vaccination strategies.MethodsA cross-sectional study was conducted using a validated anonymous questionnaire. Knowledge and attitude were measured as scores. Multivariable logistic and linear regression models were employed to identify correlates of Tdap booster and knowledge and attitude toward the vaccination, as appropriate. Models were controlled for age, sex, profession, department, and job seniority.ResultsA total of 206 questionnaires were administered among HcWs, and 143 (69.4%) were medical doctors. In total, 71 (34.47%) HcWs received the Tdap booster. Those who have worked 5–9 years at the hospital had a 78% lower likelihood of being vaccinated with the Tdap booster (5–9 years—OR: 0.22, CI: 0.06 | 0.85) as compared with newly hired HcWs. No differences in the average knowledge score were found. Other healthcare workers had a lower attitude as compared to medical doctors (Other—Coef. −2.15; CI: −4.14 | −0.15) and, as compared with those who worked in a clinical department, those who worked in a diagnostic–therapeutic department or medical management had 3.1 and 2.0 lower attitude scores, on average, respectively (diagnostic–therapeutic—Coef. −3.12, CI: −5.13 | −1.12; public health—Coef. −1.98, CI: −3.41 | −0.56).DiscussionThe study findings support the necessity to implement public health strategies and improve knowledge and attitude toward vaccinations and specifically highlight the importance of Tdap booster every 10 years as a prevention tool to protect high-risk populations.