bluuebunny/crossref_metadata_2025_split huggingface dataset:
PERTUMBUHAN BAKTERI Salmonella thypi DAPAT DIHAMBAT DENGAN AIR REBUSAN DAUN KEMANGI (Ocimum basilicum L.) TETAPI TIDAK DENGAN AIR PERASANNYA.
Authors: Alit Wardana Putu , Arsana I Nyoman , Sudaryati Ni Luh Gede 
Published: January 2023

Kemangi (Ocimum basilicum L) telah dimanfaatkan sebagai bahan obat untuk mengatasi berbagai penyakit. Penelitian ini bertujuan untuk mengetahui daya hambat air rebusan dan perasan daun kemangi terhadap pertumbuhan bakteri Salmonella thypi. Penelitian menggunakan rancangan acak kelompok dengan 12 kelompok perlakuan yaitu perasan daun kemangi konsentrasi 20%, 40%, 60%, 80%, 100%, dan rebusan daun kemangi dengan konsentrasi 20%, 40%, 60%, 80%, 100%, ditambah dengan satu kontrol positif (chloramphenicol) dan satu kontrol negatif (aquades). Rebusan daun kemangi disiapkan dengan merebus 50g daun pada suhu 90oC selama 15 menit kemudian disaring, sedangkan perasan disiapkan dengan menghancurkan 50g daun dan airnya disaring. Daya hambat ditentukan dengan medote cakram disk pada media Muller-Hilton Agar yang telah diinokulasi S. thypi. Hasil penelitian menunjukkan rebusan daun kemangi pada konsentrasi 20%, 40%, 60%, 80%, 100%, memiliki zona hambat terhadap pertumbuhan bakteri S. thypi, berturut-turut sebasar 0 mm, 8.67± 0.881 mm, 11.67±0.333 mm, 12±0.577 mm, dan 13.33±0.333 mm, sedangkan air perasan tidak memiliki zona hambat. Kesimpulan, pertumbuhan bakteri S. thypi dapat dihambat dengan rebusan daun kemangi tetapi tidak dengan air perasan.
PERILAKU SEKSUAL REMAJA DI KELURAHAN TAROK DIPO KECAMATAN GUGUAK PANJANG KOTA BUKITTINGGI
Authors: Rahmadani Suci , Yarni Linda 
Published: January 2023

Tujuan dari penelitian ni adalah untuk melihat jenis perilaku seksual remaja yang berumur 14-17 tahun di Kelurahan Tarok Dipo Kecamatan Guguk Panjang Kota Bukittinggi. Jenis penelitian yang dilakukan adalah penelitian kuantitatif yang bersifat deskriptif dengan menggambarkan, memaparkan dan menafsirkan kejadian-kejadian sesuai dengan fakta yang diperoleh yang hasilnya berupa angka-angka. Populasi remaja yang berumur 14-17 tahun di Kelurahan Tarok Dipo Kecamatan Guguk Panjang Kota Bukittinggi dalam penelitian ni adalah 624 orang, sampel yang diambil adalah sebanyak 10% dari jumlah remajamenggunakan teknik acak/randomyaitu sebanyak 62 orang. Data dikumpulkan menggunakan nstrumen angket dengan jenis Angket tertutup dan Teknik analisis data menggunakan Miscrosoft exeldan SPSS 25.0 for Windows.Hasil penelitian jenis Perilaku Seksual Remaja yang berumur 14-17 tahun Di Kelurahan Tarok Dipo Kecamatan Guguk Panjang Kota Bukittinggi terdiri atas 2 aspek yaitu aspek perilaku seksual autoerotik dan sosioseksual. Berdasarkan hasil penelitian maka didapatkan data jenis perilaku seksual yang pernah dilakukan remaja yaitu pada aspek autoerotik dengan ndikator berfantasi seksual 46%, dan ndikator mansturbasi/ onani terdapat 26,6%. Sedangkan pada aspek sosioseksual, ndikator bersentuhan/ kontak fisik dengan lawan jenis terdapat 69,4%, ndikator berpelukan terdapat sebanyak 41,4%, ndikator mencium kepala/ wajah lawan jenis terdapat 33,5%, ndikator berciuman bibir terdapat 17,7%, ndikator meraba anggota tubuh terdapat 14,1%, ndikator menempelkan kelamin terdapat 8,9% dan ndikator melakukan hubungan seksual 8,1%. Frekuensi persentase perilaku seksual yang paling banyak di lakukan oleh remaja yaitu pada perilaku bersentuhan/ kontak fisik dengan lawan jenis yaitu dengan persentase 69,4%
HUBUNGAN PENGGUNAAN SARANA DAN PRASARANA DENGAN KEEFEKTIFAN KONSELING INDIVIDU DI SMK HARAPAN BANGSA KABUPATEN PASAMAN
Authors: Nuraisyah Nuraisyah , Santosa Budi 
Published: January 2023

Latar belakang penelitina ini dikarenakan ruang BK di sekolah hanya berukuran 4m x 5m,yang mana ruang BK di dalam sekolah masih belum memadai karena belum terpenuhinya fasilitas yang dapat digunakan untuk melakukan proses konseling yang efektif seperti ruangan konseling individual, ruang konseling kelompok dan ruang konsultasi dengan orang tua siswa, dan sekolah belum menyediakan ruangan yang layak untuk melakukan konseling bagi para siswa yang ingin berkreativitas dalam kegiatan konseling seperti PIK-R. Jenis penelitian yang dilakukan adalah penelitian kuantitatif jenis korelasional melakukan penelitian lapangan (field research), yaitu mengumpulkan data langsung dari lapangan atau lokasi penelitian.Penelitian korelasional yaitu penelitian yang dimaksudkan untuk mengetahui ada tidaknya hubungan antara dua atau beberapa variabel. Data dikumpulkan dengan menggunakan teknik instrument penelitian. Metode analisis intrumen dengan menggunakan validitas instrument, reabilitas dan instrument.
Hasil penelitian yaitu sarana dan prasarana konseling sangat dibutuhkan untuk efektifitas layanan konseling yang akan dilakukan. Agar proses konseling yang dilakukan anatara guru BK dan Siswa dapat berjalan dengan baik dan terhaga kerahasiaanya. Dari efektifitas layanan konseling individu dapat disimpulkan tidak sesuai bahwa terdapat 16 orang siswa dengan persentase 23 % yang memiliki kategori sangat sesuai, 14 orang siswa dengan jumlah persentase 54% dengan kategori sesuai, 6 orang siswa memperoleh persentase 23%. data variabel penggunaan sarana dan prasarana kondengan dengan keefektifan layanan konseling individu dapat didistribusikan secara normal. hubungan yang positif dan signifikan antara penggunaan sarana dan prasarana konseling dengan keefektifan layanan konseling individu di SMK Harapan Bangsa Pasaman sebesar 0,540. Perolehan koefisien korelasi sebesar 0,540 jika dikonveksikan dengan tabel interpretasi nilai “r” maka termasuk kategori sesuai
Group farming and productivity in the Muda Agricultural Development Authority of Malaysia
Authors: Ali Jamal , Kamaruddin Roslina , Mohd Saad Nariman 
Published: January 2023

The paddy sector is a strategic sector in Malaysia. This is due to its importance in the context of rice self-sufficiency, income generation and job opportunities especially for the rural population. The Malaysian government has set the goal of achieving self- sufficiency level in rice production at about 65-70% of local consumption. In order to achieve the objective, the government has implemented many strategies, the group farming project in Muda Agricultural Development Area (MADA) is one such strategy. The study was conducted on four MADA areas representing three schemes, namely the 10 tones Project, Estate Paddy Project, and Model Farm Project. The proportionate multistage stratified random sampling technique involving the area sampling method and the systematic method was employed to select 664 respondents. An econometric model and other statistical techniques were used to estimate the effects of group farming project on productivity and farmersâ€™ income. The study shows that, in terms of economic return dimension, Model Farm Project is more successful in increasing the paddy production. The study makes a number of suggestions for improving the effectiveness of group farming projects. Recommendations are included in the project planning and implementation of group farming, and the importance of subsidy on paddy production for farmers in the study area.
Efforts and challenges in adopting international financial reporting standards (IFRS) in Nigeria
Authors: Alkali Muhammad Yusuf , Lode Nor Asma 
Published: January 2023

Financial reporting in emerging and developed economies has been a challenging
debate, due to the frequent changes as well as updates on accounting rules and
regulations. Nigeria, being an emerging economy has witnessed a dramatic change
and improvement on the accounting reporting since after political development of
the country in 1960. The establishment of Nigerian Security Exchange (NSE) in 1982
and the establishment of Nigerian Accounting Standard Board (NASB) in 1984 by the
Institute
of Chartered Accounting of Nigeria (ICAN) who were the pioneer legislations
on the road map to financial reporting in Nigeria. Even though laws and regulations
were also issued by the regulatory bodies in Nigeria to ensure quality(value relevance)
financia
l reporting, the level of compliance with the regulations have been very low
as reported by the World Bank in the 2004 and 2011(ROSC report). Subsequently,
professionals, regulatory bodies and government made the first effort by establishing
the road map a
nd the World Bank committee for the adoption of the International
Financial Reporting Standards (IFRS) in 2010. Secondly, the formation of the Financial
Reporting Council (FRC) in 2011 and FRC training institute in 2012. However, these
efforts by the gover
nment, World Bank and regulators were faced with the challenges on
the development of regulatory and legal framework as well as training of professionals
and regulators on the new accounting standard (IFRS). Therefore, this paper explores
two main issues:
(i) the efforts of government, professional bodies, and regulatory
agencies by providing accounting framework and trainings; and (ii) challenges in
adopting IFRS due to weaknesses of SAS and unethical behaviours of the preparers of
financial statements.
Vac14 protein multimerization is a prerequisite step for Fab1 protein complex assembly and function
Authors: Alghamdi Tamadher A. , Ho Cheuk Y , Mrakovic Amra , Taylor Danielle , Mao Daniel , Botelho Roberto 
Published: January 2023

<p>Phosphatidylinositol 3,5-bisphosphate (PtdIns(3,5)P2) helps control various endolysosome functions including organelle morphology, membrane recycling, and ion transport. Further highlighting its importance, PtdIns(3,5)P2 misregulation leads to the development of neurodegenerative diseases like Charcot-Marie-Tooth disease. The Fab1/PIKfyve lipid kinase phosphorylates PtdIns(3)P into PtdIns(3,5)P2 whereas the Fig4/Sac3 lipid phosphatase antagonizes this reaction. Interestingly, Fab1 and Fig4 form a common protein complex that coordinates synthesis and degradation of PtdIns(3,5)P2 by a poorly understood process. Assembly of the Fab1 complex requires Vac14/ArPIKfyve, a multimeric scaffolding adaptor protein that coordinates synthesis and turnover of PtdIns(3,5)P2. However, the properties and function of Vac14 multimerization remain mostly uncharacterized. Here we identify several conserved C-terminal motifs on Vac14 required for self-interaction and provide evidence that Vac14 likely forms a dimer. We also show that monomeric Vac14 mutants do not support interaction with Fab1 or Fig4, suggesting that Vac14 multimerization is likely the first molecular event in the assembly of the Fab1 complex. Finally, we show that cells expressing monomeric Vac14 mutants have enlarged vacuoles that do not fragment after hyperosmotic shock, which indicates that PtdIns(3,5)P2 levels are greatly abated. Therefore, our observations support an essential role for the Vac14 homocomplex in controlling PtdIns(3,5)P2 levels.</p>
<p> </p>
Radiolabeling and Quantification of Cellular Levels of Phosphoinositides by High Performance Liquid Chromatography-coupled Flow Scintillation
Authors: Ho Cheuk Y , Choy Christopher H , Botelho Roberto 
Published: January 2023

<p>Phosphoinositides (PtdInsPs) are essential signaling lipids responsible for recruiting specific effectors and conferring organelles with molecular identity and function. Each of the seven PtdInsPs varies in their distribution and abundance, which are tightly regulated by specific kinases and phosphatases. The abundance of PtdInsPs can change abruptly in response to various signaling events or disturbance of the regulatory machinery. To understand how these events lead to changes in the amount of PtdInsPs and their resulting impact, it is important to quantify PtdInsP levels before and after a signaling event or between control and abnormal conditions. However, due to their low abundance and similarity, quantifying the relative amounts of each PtdInsP can be challenging. This article describes a method for quantifying PtdInsP levels by metabolically labeling cells with H-3-myo-inositol, which is incorporated into PtdInsPs. Phospholipids are then precipitated and deacylated. The resulting soluble H-3-glycero-inositides are further extracted, separated by high-performance liquid chromatography (HPLC), and detected by flow scintillation. The labeling and processing of yeast samples is described in detail, as well as the instrumental setup for the HPLC and flow scintillator. Despite losing structural information regarding acyl chain content, this method is sensitive and can be optimized to concurrently quantify all seven PtdInsPs in cells. </p>
Reactive oxygen species prevent lysosome coalescence during PIKfyve inhibition
Authors: Saffi Golam T. , Tang Evan , Mamand Sami , Inpanathan Subothan , Fountain Aaron , Salmena Leonardo , Botelho Roberto 
Published: January 2023

<p>Lysosomes are terminal, degradative organelles of the endosomal pathway that undergo repeated fusion-fission cycles with themselves, endosomes, phagosomes, and autophagosomes. Lysosome number and size depends on balanced fusion and fission rates. Thus, conditions that favour fusion over fission can reduce lysosome numbers while enlarging their size. Conversely, favouring fission over fusion may cause lysosome fragmentation and increase their numbers. PIKfyve is a phosphoinositide kinase that generates phosphatidylinositol-3,5-bisphosphate to modulate lysosomal functions. PIKfyve inhibition causes an increase in lysosome size and reduction in lysosome number, consistent with lysosome coalescence. This is thought to proceed through reduced lysosome reformation and/or fission after fusion with endosomes or other lysosomes. Previously, we observed that photo-damage during live-cell imaging prevented lysosome coalescence during PIKfyve inhibition. Thus, we postulated that lysosome fusion and/or fission dynamics are affected by reactive oxygen species (ROS). Here, we show that ROS generated by various independent mechanisms all impaired lysosome coalescence during PIKfyve inhibition and promoted lysosome fragmentation during PIKfyve re-activation. However, depending on the ROS species or mode of production, lysosome dynamics were affected distinctly. H2O2 impaired lysosome motility and reduced lysosome fusion with phagosomes, suggesting that H2O2 reduces lysosome fusogenecity. In comparison, inhibitors of oxidative phosphorylation, thiol groups, glutathione, or thioredoxin, did not impair lysosome motility but instead promoted clearance of actin puncta on lysosomes formed during PIKfyve inhibition. Additionally, actin depolymerizing agents prevented lysosome coalescence during PIKfyve inhibition. Thus, we discovered that ROS can generally prevent lysosome coalescence during PIKfyve inhibition using distinct mechanisms depending on the type of ROS.</p>
<p> </p>
Low oxytocin levels are broadly associated with more pronounced psychopathology in anorexia nervosa with primarily restricting but not binge/purge eating behavior
Authors: Plessow Franziska , Galbiati Francesca , Eddy Kamryn T. , Misra Madhusmita , Miller Karen K. , Klibanski Anne , Aulinas Anna , Lawson Elizabeth A. 
Published: January 2023

ObjectiveAnorexia nervosa (AN) is commonly associated with depression, anxiety, and deficits in socioemotional functioning. Basal levels of oxytocin, a neurohormone with antidepressant, anxiolytic, and prosocial properties, are low in women with AN. However, the relationship between oxytocin and psychopathology of AN/atypical AN has not been examined in individuals with primarily food restriction (AN/AtypAN-R) or those with restriction plus binge/purge behaviors (AN/AtypAN-BP) alone, which is important to further elucidate the neurobiology of different AN presentations. We investigated whether oxytocin levels are related to eating, affective, and socioemotional psychopathology in women with AN/AtypAN-R and separately AN/AtypAN-BP.MethodsIn a cross-sectional study of 53 women with low-weight AN or atypical AN based on DSM-5 (AN/AtypAN-R: n=21, AN/AtypAN-BP: n=32), we obtained fasting serum oxytocin levels and self-report measures of psychopathology, including the Eating Disorder Examination–Questionnaire (EDE-Q), Beck Depression Inventory-IA (BDI), State-Trait Anxiety Inventory (STAI), and Toronto Alexithymia Scale (TAS-20).ResultsIn individuals with AN/AtypAN-R, oxytocin levels were negatively associated with eating psychopathology (EDE-Q Global Score: r=-0.49, p=0.024), depressive and anxiety symptoms (BDI Total Score: r=-0.55, p=0.009; STAI Trait Score: r=-0.63, p=0.002), and socioemotional symptoms (TAS-20 Difficulty Identifying Feelings Score: r=-0.49, p=0.023). In contrast, in those with AN/AtypAN-BP oxytocin levels were negatively associated with depressive symptoms only (BDI Total Score: r=-0.52, p=0.049).ConclusionsThese findings support the notion that AN/AtypAN-R and AN/AtypAN-BP might have divergent underlying neurobiology. Understanding these differences is crucial to develop targeted treatments for a population with high levels of chronicity, for which no specific pharmacological treatments are currently available.Clinical trial registrationhttps://clinicaltrials.gov, identifier: NCT01121211.
Protective effects of melatonin against physical injuries to testicular tissue: A systematic review and meta-analysis of animal models
Authors: Dehdari Ebrahimi Niloofar , Shojaei-Zarghani Sara , Taherifard Ehsan , Dastghaib Sanaz , Parsa Shima , Mohammadi Nasim , Sabet Sarvestani Fatemeh , Moayedfard Zahra , Hosseini Nima , Safarpour Heidar , Sadeghi Alireza , Azarpira Negar , Safarpour Ali Reza 
Published: January 2023

BackgroundModern societies face infertility as a global challenge. There are certain environmental conditions and disorders that damage testicular tissue and may cause male infertility. Melatonin, as a potential antioxidant, may protect testicular tissue. Therefore, we conducted this systematic review and meta-analysis to evaluate the effects of melatonin in animal models against physical, heat, and ischemic damage to the testicular tissue.MethodsPubMed, Scopus, and Web of Science were systematically searched to identify animal trials evaluating the protective effect of melatonin therapy on rodent testicular tissue when it is exposed to physical, thermal, ischemic, or hypobaric oxygen stress. Random-effect modeling was used to estimate the standardized mean difference and 95% confidence intervals based on the pooled data. Additionally, the Systematic Review Centre for Laboratory Animal Experimentation (SYRCLE) tool was used to assess the risk of bias. The study protocol was prospectively registered in PROSPERO (CRD42022354599).ResultsA total of 41 studies were eligible for review out of 10039 records. Studies employed direct heat, cryptorchidism, varicocele, torsion-detorsion, testicular vascular occlusion, hypobaric hypoxia, ischemia-reperfusion, stress by excessive or restraint activity, spinal cord injury, and trauma to induce stress in the subjects. The histopathological characteristics of testicular tissue were generally improved in rodents by melatonin therapy. Based on the pooled data, sperm count, morphology, forward motility, viability, Johnsen’s biopsy score, testicular tissue glutathione peroxidase, and superoxide dismutase levels were higher in the melatonin treatment rodent arms. In contrast, the malondialdehyde level in testicular tissue was lower in the treatment rodent arms. The included studies suffered from a high risk of bias in most of the SYRCLE domains.ConclusionThis study concludes that melatonin therapy was associated with improved testicular histopathological characteristics, reproductive hormonal panel, and tissue markers of oxidative stress in male rodents with physical, ischemic, and thermal testicular injuries. In this regard, melatonin deserves scientific investigations as a potential protective drug against rodent male infertility.Systematic review registrationhttps://www.crd.york.ac.uk/PROSPERO/, identifier CRD42022354599.
Simulation and economic analysis of the high-temperature heat storage system of thermal power plants oriented to the smart grid
Authors: Zhang Hongwei , Zhao Mingyuan , Li Yanbing , Liu Junqing , Liang Wenbin 
Published: January 2023

With the continuous increase of the grid-connected proportion of intermittent renewable energy, in order to ensure the reliability of smart grid operation, it is urgent to improve the operational flexibility of thermal power plants. Electric heat storage technology has broad prospects in terms of in-depth peak shaving of power grids, improving new energy utilization rates and improving the environment. It is an important means to promote electric energy substitution. In this study, the economics of technical application scenarios are compared and analyzed, the principle of solid heat storage technology is discussed, and its application in heating fields such as industrial steam, district heating, and deep peak regulation of congeneration units is expounded. The results indicate that in the scenario where the peak shaving subsidy and the heat storage duration are the same, as the unit output increases, the investment recovery period increases. Moreover, the results also indicate that in the 0.3 yuan/kW power market peaking subsidy scenario, only when the unit output is 0 and the heat storage time is greater than 8 h, the investment can be recovered in 5 years, while in the 0.7 yuan/kW power market peaking subsidy scenario, except for the scenario where the unit output is 40% and the heat storage time is 7 h, the investment cannot be recovered; in other scenarios, the investment can be recovered within 5 years.
Gender- and age-specific associations of childhood maltreatment with peripheral serum inflammatory cytokines in middle school students
Authors: Jin Zhengge , Li Shuqin , Li Ruoyu , Song Xianbing , Zhang Shichen , Sun Ying , Tao Fangbiao , Wan Yuhui 
Published: January 2023

BackgroundThe impact of childhood maltreatment on multiple inflammatory cytokines among middle school students remains to be elucidated. This study aimed to examine the associations of different types of childhood maltreatment with peripheral serum inflammatory cytokines (interleukin-10, interleukin-1β, interleukin-6, interleukin-8, and tumor necrosis factor-α) in middle school students, and to explore the differences in these associations between boys and girls and between late (≥15 and&lt;20 years) and early (≥11 and &lt;15 years) adolescence.MethodsA total of 1122 students were recruited from a boarding middle school. Each participant was asked to respond to a detailed questionnaire on childhood maltreatment, from whom one blood sample was drawn via venous blood.ResultsIn the overall sample there was no association between childhood maltreatment and peripheral serum inflammatory cytokines; (2) emotional abuse was significantly correlated with IL-1β only in girls (B = -0.16; 95% CI, -0.28~-0.03; p = 0.06); (3) in late adolescence, emotional abuse, emotional neglect, and childhood maltreatment had marked link with IL-8 (B = 0.39; 95%CI, 0.16~0.63; p = 0.01; B =0.20; 95% CI, 0.04~0.37; p = 0.08; B = 0.50; 95% CI, 0.18~0.82; p = 0.01, respectively).ConclusionThese findings also strengthened an inference regarding the effects of childhood maltreatment on inflammation of students in late adolescence.