bluuebunny/crossref_metadata_2025_split huggingface dataset:
Phytochemical Profiling and Compound Isolation of Cissus rhombifolia Vahl. Leaves Aqueous Methanolic Extract with the Evaluation of Its Anti‐Inflammatory Effect Using Lipopolysaccharide‐Induced Inflammation in RAW 264.7 Cells
Authors: Raslan Mona A. , Mounier Marwa M. 
Published: May 2023

AbstractThe inflammatory disorders represent a serious health issue. Certain Cissus species possess anti‐inflammatory effect. Cissus rhombifolia Vahl. leaves’ anti‐inflammatory activities and phytoconstituents are poorly characterized. In this study, 38 constituents were tentatively characterized in Cissus rhombifolia Vahl. leaves’ aqueous methanolic extract (CRLE) using high‐performance liquid chromatography combined with mass spectrometry (HPLC/MS) and Proton Nuclear Magnetic Resonance (1H‐NMR). Myricetin, β‐amyrin, and alliospiroside A, were isolated from CRLE using column chromatography. The anti‐inflammatory effect of CRLE and its isolated compounds were studied in lipopolysaccharide (LPS)‐induced RAW 264.7 cells. 3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyltetrazolium bromide (MTT assay) was used to assess how CRLE and its isolated compounds affected cell viability. Further, its effects on the production of intracellular NO, and inflammatory cytokines cyclooxygenase‐2 (COX‐2), tumor necrosis factor alpha (TNF‐α), and interleukin 6 (IL‐6) were assessed by the Griess test, and cytokine enzyme‐linked immunosorbent assays, respectively. CRLE and its isolated compounds, myricetin, β‐amyrin, and alliospiroside A decreased the NO production. Western blotting was performed to assess the protein expression levels of the inflammatory cytokines inducible nitric oxide synthase (iNOS). Alliospiroside A downregulated IL‐6, TNF‐α, and COX‐2 and inhibited the expression of iNOS. CRLE and its compounds represent effective alternative candidate to treat inflammatory diseases.
Pantoea abscess mimicking sarcoma in a HTLV‐1‐infected Indigenous Australian man: Case report and literature review
Authors: Susanto Maja , Dunning Jacki , Chew Rusheng 
Published: May 2023

Key Clinical MessageGram‐negative bacteria of the genus Pantoea are emerging bacterial causes of diverse sporadic and outbreak‐linked infections. Chronic Pantoea abscesses are unusual and may give rise to a differential diagnosis of malignancy. Foreign body retention and host immune defects may be risk factors for such chronic infections.
Chronic diarrhea as a presentation of Behçet's disease
Authors: Elmassry Marawan , Matar Sayed , Thongpiya Jerapas , Yingchoncharoen Pitchaporn , Abohelwa Mostafa , Islam Sameer 
Published: May 2023

Key Clinical MessagesBehçet's disease (BD) or syndrome is a chronic, recurrent, multisystem, inflammatory vasculitis disorder with findings of oral aphthous ulcers, genital ulcers, and uveitis. Gastrointestinal (GI) involvement can be the initial presentation as presented in this case.AbstractBehçet's disease (BD) or syndrome is a chronic, recurrent, multisystem, inflammatory vasculitis disorder of unknown etiology with classical findings of oral aphthous ulcers, genital ulcers, and ocular involvements including chronic anterior, intermediate, posterior, and even panuveitis. Gastrointestinal involvement in BD usually presents with chronic diarrhea, hematochezia as the disease affects ileocecal area which might be similar to presentation of inflammatory bowel diseases. Here, we report a case of undiagnosed BD who presented with chronic diarrhea for 4 months, leading to the diagnosis of BD and responded well to corticosteroid therapy.
Parental choice and seed size impact the uprightness of progeny from interspecific Glycine hybridizations
Authors: Taliercio Earl , Eickholt David , Read Quentin D. , Carter Thomas , Waldeck Nathan , Fallen Ben 
Published: May 2023

AbstractThe narrow genetic base of the US soybean (Glycine max [L.] Merr.) crop makes it vulnerable to emerging abiotic and biotic stress challenges, and limits resiliency of the soybean crop to meet changes in consumer demand for improved seed composition and agronomic performance. The United States Department of Agriculture Glycine soja (Siebold & Zucc.) germplasm collection provides a valuable genetic resource to meet these challenges. Glycine soja is more genetically diverse than domesticated soybean. Even though wild soybean hybridizes freely with G. max, breeding efforts with wild soybean have not been widely employed because the interspecific progeny inherits undesirable traits from the wild parent. Particularly, these progenies inherit a vine‐like architecture that prevents machine harvest. We assessed the potential for improving recovery of agronomically valuable progeny from interspecific crosses in three experiments using 11 interspecific populations. We found that optimal choice of either parent could triple the frequency of recovery of desirable progeny. Selection for large F3 seed size was positively correlated with upright growth habit/plant architecture in five of 11 populations and could triple the recovery of desirable plants. Marker analysis of parents and progeny indicated selection for larger seed size caused minimal selection against the genome of the wild parent. Most genetic markers specific to a wild parent could be recovered in the aggregate of 8–10 upright interspecific progenies, identified via post selection marker analysis. We concluded that choice of parent and selection for larger seed can maximize recovery of the wild genome in machine harvestable progenies.
Divergent selection for timing of vegetative phase change
Authors: Branch Carl A. , Tracy William F. 
Published: May 2023

AbstractVegetative phase change is a key trait in plant development and marks the transition from juvenile to adult growth phases. In maize (Zea mays L.), juvenile plants and plants with a late phase change can be identified by the increased production of tillers, aerial roots, and distinctive epicuticular wax. Resistance to agronomically significant pathogens can also differ between juvenile and adult plants. Using last leaf with juvenile wax as the target of selection, the maize population Minnesota 11 underwent 16 cycles of divergent selection. The objectives of this research were to identify emergent trends for each trait and to determine the magnitude and direction of phenotypic changes over long‐term selection compared to the source population. Phenotypic data on plant architecture traits, ear traits, and common rust resistance were collected in 2020 and 2021 by concurrently growing plants from selection cycles representing both directions of selection. Last leaf with juvenile wax, the direct target of selection increased to leaf 17.8 in the late phase change population and decreased to leaf 5 in the early phase change population from leaf 9 in the source population. Late vegetative phase change was positively correlated with increased common rust infection and plant height, and negatively correlated with ear size traits. This study shows that the observed phenotypic changes follow patterns of genetic variation consistent with divergent selection, but with possible effects of inbreeding.
Biodiversity protection against anthropogenic climate change: Conservation prioritization of Castanea sativa in the South Caucasus based on genetic and ecological metrics
Authors: Beridze Berika , Sękiewicz Katarzyna , Walas Łukasz , Thomas Peter A. , Danelia Irina , Fazaliyev Vahid , Kvartskhava Giorgi , Sós Jan , Dering Monika 
Published: May 2023

AbstractThe climate drives species distribution and genetic diversity; the latter defines the adaptability of populations and species. The ongoing climate crisis induces tree decline in many regions, compromising the mitigation potential of forests. Scientific‐based strategies for prioritizing forest tree populations are critical to managing the impact of climate change. Identifying future climate refugia, which are locations naturally buffering the negative impact of climate change, may facilitate local conservation. In this work, we conducted the populations' prioritization for Castanea sativa (sweet chestnut), a Neogene relict growing in the Caucasus global biodiversity hotspot. We generated genetic and ecological metrics for 21 sites in Georgia and Azerbaijan, which cover the natural range of sweet chestnut across the region. We demonstrated that climate primarily drives the pattern of genetic diversity in C. sativa, proved with a significant isolation‐by‐environment model. In future, climate change may significantly reorganize the species' genetic diversity, inducing even some genetic loss, especially in the very distinct eastern fringe of the species range in Azerbaijan. Based on our combined approach, we mapped populations suitable for ex situ and in situ conservation, accounting for genetic variability and the location of future climate refugia.
Patterns and drivers of soil surface‐dwelling Oribatida diversity along an altitudinal gradient on the Changbai Mountain, China
Authors: Lin Yiling , Wu Haitao , Liu Dong , Li Yaxiao , Kang Yujuan , Zhang Zhongsheng , Wang Wenfeng 
Published: May 2023

AbstractDistribution patterns of biodiversity and environmental interactions are dominant themes in ecology. In montane ecosystems, biodiversity is closely associated with altitudinal gradients. However, studies of biodiversity in montane ecosystems are focused on plants and vertebrates, with relatively less on invertebrates. Here, the present study used a Vortis arthropod suction sampler to explore the biodiversity patterns of soil surface‐dwelling Oribatida and their drivers along an altitudinal gradient (600, 800, 1600, 2000, and 2300 m) from typical temperate forests, evergreen coniferous forests, subalpine birch forests to alpine tundra on the north slope of Changbai Mountain, Northeast China. Trichoribates berlesei, Platynothrus peltifer, and Oribatula tibialis were the dominant soil surface‐dwelling species on Changbai Mountain. Generally, alpha diversity and beta diversity of soil surface‐dwelling Oribatida decreased with the rising altitude, with a peaking density value at 2000 m. The result of beta diversity showed that the structures of community were more influenced by the species turnover component than the nestedness component. Nonmetric multidimensional scaling (NMDS) ordination showed that the community structure of soil surface‐dwelling Oribatida varied significantly along the altitudinal gradient. The variance partitioning showed that the elevation and climatic conditions determined the soil surface‐dwelling Oribatida community. Spatial filtering represented by geographic and elevation distances was particularly associated with soil surface‐dwelling Oribatida community variation between altitudes on Changbai Mountain. However, the variation of the Oribatida community between adjacent altitudes was only associated with geographic distance. Our study provides supportive evidence for the biodiversity analyzing of soil surface‐dwelling Oribatida in montane ecosystems along an altitudinal gradient.
Patient phenotype profiling in heart failure with preserved ejection fraction to guide therapeutic decision making. A scientific statement of the Heart Failure Association, the European Heart Rhythm Association of the European Society of Cardiology, and the European Society of Hypertension
Authors: Anker Stefan D. , Usman Muhammad Shariq , Anker Markus S. , Butler Javed , Böhm Michael , Abraham William T. , Adamo Marianna , Chopra Vijay K. , Cicoira Mariantonietta , Cosentino Francesco , Filippatos Gerasimos , Jankowska Ewa A. , Lund Lars H. , Moura Brenda , Mullens Wilfried , Pieske Burkert , Ponikowski Piotr , Gonzalez‐Juanatey Jose R. , Rakisheva Amina , Savarese Gianluigi , Seferovic Petar , Teerlink John R. , Tschöpe Carsten , Volterrani Maurizio , von Haehling Stephan , Zhang Jian , Zhang Yuhui , Bauersachs Johann , Landmesser Ulf , Zieroth Shelley , Tsioufis Konstantinos , Bayes‐Genis Antoni , Chioncel Ovidiu , Andreotti Felicita , Agabiti‐Rosei Enrico , Merino Jose L. , Metra Marco , Coats Andrew J.S. , Rosano Giuseppe M.C. 
Published: May 2023

AbstractHeart failure with preserved ejection fraction (HFpEF) represents a highly heterogeneous clinical syndrome affected in its development and progression by many comorbidities. The left ventricular diastolic dysfunction may be a manifestation of various combinations of cardiovascular, metabolic, pulmonary, renal, and geriatric conditions. Thus, in addition to treatment with sodium–glucose cotransporter 2 inhibitors in all patients, the most effective method of improving clinical outcomes may be therapy tailored to each patient's clinical profile. To better outline a phenotype‐based approach for the treatment of HFpEF, in this joint position paper, the Heart Failure Association of the European Society of Cardiology, the European Heart Rhythm Association and the European Hypertension Society, have developed an algorithm to identify the most common HFpEF phenotypes and identify the evidence‐based treatment strategy for each, while taking into account the complexities of multiple comorbidities and polypharmacy.
Sudden unexpected death in epilepsy: What Saudi people with epilepsy want to know—A cross‐sectional study
Authors: Aljumah Talal K. , Al‐Attas Alawi A. , Alqahtani Ahmed A. , Masuadi Emad M. , Aldahi Ahmed S. , Alshammari Mohammed A. , Heji Alaa T. , Alanazi Ali 
Published: May 2023

AbstractObjectiveSudden unexpected death in epilepsy (SUDEP) is a significant cause of mortality in people with epilepsy (PWE), with an incidence of 1 per 1000 members of the population. In Saudi Arabia, no data are available that inform local clinical practitioners about the attitudes of PWE regarding SUDEP. The aim of this study was to investigate the perspectives of Saudi PWE toward SUDEP and to assess their knowledge of SUDEP.MethodsA cross‐sectional questionnaire‐based study was conducted at the neurology clinics of King Abdul‐Aziz Medical City, Riyadh and Prince Sultan Military Medical City, Riyadh.ResultsOf the 377 patients who met the inclusion criteria, 325 completed the questionnaire. The mean age of the respondents was 32.9 ± 12.6 years. Of the study subjects, 50.5% were male. Only 41 patients (12.6%) had heard about SUDEP. Most patients (94.5%) wanted to know about SUDEP, of whom 313 (96.3%) wanted to receive this information from a neurologist. A total of 148 patients (45.5%) thought that the appropriate time to receive information about SUDEP was after the second visit, whereas only 75 (23.1%) wanted to learn about SUDEP during the first visit. However, 69 patients (21.2%) thought that the appropriate time to be informed about SUDEP was when seizure control had become more difficult. Almost half (172, 52.9%) of the patients thought that SUDEP could be prevented.SignificanceOur findings suggest that most Saudi PWE do not know about SUDEP and want to be counseled about their risk of SUDEP by their physicians. Therefore, education of Saudi PWE about SUDEP must be improved.
Understanding Ecological Complexity in a Chemical Stress Context: A Reflection on Recolonization, Recovery, and Adaptation of Aquatic Populations and Communities
Authors: Bundschuh Mirco , Mesquita-Joanes Francesc , Rico Andreu , Camacho Antonio 
Published: May 2023

Abstract
Recovery, recolonization, and adaptation in a chemical stress context are processes that regenerate local populations and communities as well as the functions these communities perform. Recolonization, either by species previously present or by new species able to occupy the niches left empty, refers to a metacommunity process with stressed ecosystems benefiting from the dispersal of organisms from other areas. A potential consequence of recolonization is a limited capacity of local populations to adapt to potentially repeating events of chemical stress exposure when their niches have been effectively occupied by the new colonizers or by new genetic lineages of the taxa previously present. Recovery, instead, is an internal process occurring within stressed ecosystems. More specifically, the impact of a stressor on a community benefits less sensitive individuals of a local population as well as less sensitive taxa within a community. Finally, adaptation refers to phenotypic and, sometimes, genetic changes at the individual and population levels, allowing the permanence of individuals of previously existing taxa without necessarily changing the community taxonomic composition (i.e., not replacing sensitive species). Because these processes are usually operating in parallel in nature, though at different degrees, it seems relevant to try to understand their relative importance for the regeneration of community structure and ecosystem functioning after chemical exposure. In the present critical perspective, we employed case studies supporting our understanding of the underlying processes with the hope to provide a theoretical framework to disentangle the relevance of the three processes for the regeneration of a biological community after chemical exposure. Finally, we provide some recommendations to experimentally compare their relative importance so that the net effects of these processes can be used to parameterize risk-assessment models and inform ecosystem management. Environ Toxicol Chem 2023;42:1857–1866. © 2023 The Authors. Environmental Toxicology and Chemistry published by Wiley Periodicals LLC on behalf of SETAC.
Treatment patterns and clinical outcomes of mantle cell lymphoma: A retrospective cohort study by CHOICE
Authors: Zeng Dongfeng , Fang Yu , Fei Yue , Liang Rong , Ye Haige , Liang Yun , Sun Xiuhua , Wang Michael , Huang Huiqiang , Qiu Lugui , Che Yuxuan , Liu Panpan , Wang Yi , Pan Tao , Lv Yao , Deng Jintai , Yi Shuhua , He Yizi , Xiao Ling , Lv Huijuan , Feng Jiangfang , Zhang Huilai , Zhou Hui , Zou Dehui , Cai Qingqing 
Published: May 2023

AbstractRegimens based on Bruton's tyrosine kinase inhibitors (BTKi) have been increasingly used to treat mantle cell lymphoma (MCL). A real‐world multicenter study was conducted to characterize treatment patterns and outcomes in patients with newly diagnosed MCL by Chinese Hematologist and Oncologist Innovation Cooperation of the Excellent (CHOICE). The final analysis included 1261 patients. Immunochemotherapy was the most common first‐line treatment, including R‐CHOP in 34%, cytarabine‐containing regimens in 21% and BR in 3% of the patients. Eleven percent (n = 145) of the patients received BTKi‐based frontline therapy. Seventeen percent of the patients received maintenance rituximab. Autologous hematopoietic stem cell transplantation (AHCT) was conducted in 12% of the younger (<65 years) patients. In younger patients, propensity score matching analysis did not show significant difference in 2‐year progression‐free survival and 5‐year overall survival rate in patients receiving standard high‐dose immunochemotherapy followed by AHCT than induction therapy with BTKi‐based regimens without subsequent AHCT (72% vs 70%, P = .476 and 91% vs 84%, P = .255). In older patients, BTKi combined with bendamustine plus rituximab (BR) was associated with the lowest POD24 rate (17%) compared with BR and other BTKi‐containing regimens. In patients with resolved hepatitis B at the baseline, HBV reactivation rate was 2.3% vs 5.3% in those receiving anti‐HBV prophylaxis vs not; BTKi treatment was not associated with higher risk of HBV reactivation. In conclusion, non‐HD‐AraC chemotherapy combined with BTKi may be a viable therapeutic strategy for younger patients. Anti‐HBV prophylaxis should be implemented in patients with resolved hepatitis B.
Psoas muscle index is not representative of skeletal muscle index for evaluating cancer sarcopenia
Authors: Pigneur Frédéric , Di Palma Mario , Raynard Bruno , Guibal Aymeric , Cohen Frédéric , Daidj Nassima , Aziza Richard , El Hajjam Mostafa , Louis Guillaume , Goldwasser François , Deluche Elise 
Published: May 2023

AbstractBackgroundA common method for diagnosing sarcopenia involves estimating the muscle mass by computed tomography (CT) via measurements of the cross‐sectional muscle area (CSMA) of all muscles at the third lumbar vertebra (L3) level. Recently, single‐muscle measurements of the psoas major muscle at L3 have emerged as a surrogate for sarcopenia detection, but its reliability and accuracy remain to be demonstrated.MethodsThis prospective cross‐sectional study involved 29 healthcare establishments and recruited patients with metastatic cancers. The correlation between skeletal muscle index (SMI = CSMA of all muscles at L3/height2, cm2/m2) and psoas muscle index (PMI = CSMA of psoas at L3/height2, cm2/m2) was determined (Pearson's r). ROC curves were prepared based on SMI data from a development population (n = 488) to estimate suitable PMI thresholds. International low SMI cut‐offs according to gender were studied for males (<55cm2/m2) and for females (<39 cm2/m2). Youden's index (J) and Cohen's kappa (κ) were calculated to estimate the test's accuracy and reliability. PMI cut‐offs were validated in a validation population (n = 243) by estimating the percentage concordance of sarcopenia diagnoses with the SMI thresholds.ResultsSeven hundred and sixty‐six patients were analysed (mean age 65.0 ± 11.8 years, 50.1% female). Low SMI prevalence was 69.1%. Correlation between the SMI and PMI for the entire population was 0.69 (n = 731, P < 0.01). PMI cut‐offs for sarcopenia were estimated in the development population at <6.6cm2/m2 in males and at <4.8 cm2/m2 for females. The J and κ coefficients for PMI diagnostic tests were weak. The PMI cut‐offs were tested in the validation population where 33.3% of the PMI measurements were dichotomously discordant.ConclusionsA diagnostic test employing single‐muscle measurements of the psoas major muscle as a surrogate for sarcopenia detection was evaluated but found to be unreliable. The CSMA of all muscles must be considered for evaluating cancer sarcopenia at L3.
Gene therapies for mucopolysaccharidoses
Authors: Rossi Alessandro , Brunetti‐Pierri Nicola 
Published: May 2023

AbstractCurrent specific treatments for mucopolysaccharidoses (MPSs) include enzyme replacement therapy (ERT) and hematopoietic stem cell transplantation (HSCT). Both treatments are hampered by several limitations, including lack of efficacy on brain and skeletal manifestations, need for lifelong injections, and high costs. Therefore, more effective treatments are needed. Gene therapy in MPSs is aimed at obtaining high levels of the therapeutic enzyme in multiple tissues either by engrafted gene‐modified hematopoietic stem progenitor cells (ex vivo) or by direct infusion of a viral vector expressing the therapeutic gene (in vivo). This review focuses on the most recent clinical progress in gene therapies for MPSs. The various gene therapy approaches with their strengths and limitations are discussed.