bluuebunny/crossref_metadata_2025_split huggingface dataset:
Detection of virulence genes among Staphylococcus saprophyticus isolated from women with urinary tract infections: First report from Iran
Authors: Rafiee Maryam , Ghaemi Ezzat Allah 
Published: May 2023

Abstract

Urinary tract infections (UTIs) are among the most common infections worldwide andStaphylococcus saprophyticusis an important pathogen responsible for community-acquired urinary tract infections (CAUTI) in young women. The purpose of this study was to detect the presence of virulence genes and biochemical characteristics ofS. saprophyticusstrains isolated from women with UTI for the first time in Gorgan, Iran. A total of 35S. saprophyticusisolates were collected between May 2018 and September 2020 from women with UTI in Gorgan, northern Iran. The presence of four genes associated with the surface ofS. saprophyticus,UafA,Aas,Ssp, andSdrI, was investigated by PCR. A microtiter plate assay was performed to test biofilm formation capability. Hemagglutination and lipase activity assays were also performed.The frequencies ofUafA,Aas,Ssp, andSdrIwere 88%, 91%, 80%, and (0), respectively. The results showed that 32(91%) of the isolates formed biofilms. Moreover, seven (20%), 13 (37%), and 12(34%) isolates were categorized as weak, moderate, and strong biofilm producers, respectively. None of the isolates exhibited lipolytic activities. Regarding hemagglutination properties, only 11 (31%) isolates demonstrated hemagglutination of sheep erythrocytes. The results of this study indicate a high prevalence ofUafAandAasgenes that can enhance the pathogenicity ofS. saprophyticus, and their role in hemagglutination needs to be further studied.
Long Term Outcome of Surrogate Subtypes of Primary Operable Breast Cancer According to Securin Expression
Authors: Lovrić Marko , Plavetić Natalija Dedić , Majzec Mirna , Kulić Ana , Sirotković-Skerlev Maja , Vrbanec Damir , Jakić-Razumović Jasminka 
Published: May 2023

Abstract
Background: Despite dramatic advances in cancer research, breast cancer remains a major health problem. In this heterogeneous disease, patients with different molecular subtypes have a different therapeutic approach and prognosis. Securin is known to participate in maintaining chromosomal integrity during the cell cycle through regulation of metaphase-anaphase transition, DNA damage repair and apoptosis. The aim of this study was to evaluate the prognostic role of securin expression as a measure of chromosomal instability in different surrogate subtypes of breast cancer in order to discriminate patients with worse prognosis.
Materials and methods: Breast cancer paraffin-embedded tissue specimens were obtained from a consecutive series of 215 patients with primary operable invasive breast carcinomas referred to the University Hospital Centre Zagreb, Croatia, from 2002 to 2003. Immunohistochemical (IHC) staining for securin was performed, and all relevant clinical and histopathological data were collected. Surrogate subtypes were defined according to St Gallen's consensus criteria. All patients were followed-up prospectively according to standard institutional practise for local and distant reccurence and death to collect data on disease-free (DFS) and overall survival (OS).
Results: In this patient cohort, median securin expression was 7 % of positive cells, ranging from 1 % to 42 %. Statistically significant correlation between tumor size and securin expression (p = 0.0272) and securin and Ki 67% expression (p = 0.0065) was shown. Securin expression differs among surrogate subtypes of breast cancer with highest expression in HER2+ subtype (median= 12). Univariate analysis has shown that in luminal A subtype and triple negative subtype there is statistically significant correlation between securin expression and DFS as well as OS. According to logistic regression analysis, it has also been shown that securin expression was independent prognostic factor for ten-year overall survival (OS).
Conclusion: Our study has shown that patients with BC overexpressing securin have a worse long-term prognosis in comparison with those without overexpression but only in luminal A like and triple negative like surrogate subtypes.
Lanthanide tetrad effects in stream sediment rich- gold of Betare Oya area (Cameroon Pan-African fold belt, Betare Oya gold district): implication for REE- bearing phases fractionation
Authors: Jean-Lavenir Ndema Mbongue , Agyingi Christopher Mbaringong , Cyrille Sigue , Estelle Nga Essomba Tsoungui Philomène , Blessing Elong Fanwi Christelle , Enowakwen Tanyi Lineslie , Fuh Ndongfong Edson 
Published: May 2023

Abstract
Stream sediment geochemical surveys were carried out in Betare Oya in the Cameroon Pan-African Fold Belt. Samples collected were analyzed by ICP-MS for major, trace and rare earth element contents. The purpose of this research was to investigate the geochemical behavior of lanthanides in sediments and their implication for REE-bearing phase formation. Sediments exhibit higher content in Fe2O3 (3.96–21.30%), Au (133->10000 ppb), Hg (4000->10000 ppb), Mn (868–2950), Pb (20.96–2950 ppm). High REE (510.7-3340.2 ppm) and Y contents were delivered from Betare Oya, the UCC- normalized REE patterns show LREE enrichment (LaN/SmN: 0.81–1.32) relative to HREE (GdN/YbN: 2.26–15.32), with a negative Eu anomaly (Eu/Eu*: 0.15–0.29). REE patterns exhibit tetrad effects showing W- and M- types. The plots of the sizes of tetrad effects with some geochemical parameters allow to characterize two distinct groups of population: the first group involved majority of samples and the second group encompasses few samples indicating samples were less affected by terrigenous source materials. The two groups are related to tetrad effect, they can serve as geochemical indicator to determine the environmental conditions. Samples of group 2 have significant tetrad effects (1.88–2.28) and sediments indicate the non-CHARAC behavior. The enrichment of LREE compared to HREE is due to the presence of monazite. The result of this research serves as new evidence for REE dynamics in Cameroon, the values of REE can be used as background values for further investigation.
Effects of Bacillus coagulans and Lactobacillus plantarum on the Fermentation Quality, Aerobic Stability and Microbial Community of Triticale Silage
Authors: Li Shengnan , Ke Wencan , Zhang Qing , Undersander Dan , Zhang Guijie 
Published: May 2023

Abstract
The present study investigated the impact of L. plantarum and B. coagulans on the fermentation characteristics, microbial community, and aerobic stability of ensiled triticale. Fresh triticale was wilted at a dry matter content of 35%. The treatments were arranged in a 2 × 2 factorial design, with both Lactobacillus plantarum (LP) and Bacillus coagulans 21735(BC) added at 0 or 1 × 106 cfu/g of fresh forage (FW). After 60 d of ensiling, the pH, water-soluble carbohydrates (WSC), neutral detergent fiber (NDF), and ammonia nitrogen (NH3-N) of inoculated groups were lower than those of the control group (P < 0.05), especially in the LP + BC treatment (P < 0.05). The lactic acid (LA) concentration, lactic acid/acetic acid (LA/AA), and aerobic stability were also higher (P < 0.05) in the LP + BC treatment than in other treatments. The bacterial diversity was reduced, and the richness was increased by the application of LP and BC individually (P < 0.05). Compared with the control silage, LP-treated silage had higher Lactobacillus (P < 0.05), while BC-treated silage had higher Bacillus and Pediococcus (P < 0.05). The LP + BC-treated silage had higher Lactobacillus, Bacillus, Enterococcus and Serratia (P < 0.05). Bacillus was negatively associated with NDF (P < 0.05) and AA (P < 0.05). Lactobacillus was positively associated with LA (P < 0.05) and LA/AA but negatively with pH and NH3-N (P < 0.05). In conclusion, the combination of BC and LP may yield beneficial outcomes in terms of ensiled triticale fermentation quality and aerobic stability by inducing alterations in the composition of bacterial communities, which is crucial for the efficient utilization of triticale resources.
Evaluation of the Antimicrobial, Antioxidant andAnti-inflammatory Effects of Thyme Extract Against Periopathogens – An In vitro Study
Authors: Mahale Swapna , Gattani Krishnapriya 
Published: May 2023

Abstract
BACKGROUND
People globally are spiraling to herbal products to reconnect with nature over cost efficiency and minimal side effects. Thyme is a beneficial herbal plant with antimicrobial, antioxidant and anti-inflammatory effect. Thymol has a suppressor effect on the growth of oral bacteria which helps in the reduction of decay. Antiseptic, expectorant, and antispasmodic activities are also documented to thyme extract .Our study aim was to assess the effect of antimicrobial, antioxidant and anti-inflammatory effect of thyme extract via an in-vitro analysis against periodontal pathogens(P.g, A.a, F.n) .
MATERIAL AND METHODS
The thyme extract was prepared using soxhlet extractor. Minimum Inhibitory Concentration, and Minimum Bactericidal Concentration (MIC, MBC) was estimated using thioglycollate broth through serial dilution, whereas antioxidant effect was measured using DPPH radical scavenging activity. Anti-inflammatory effect was gauged using electrophoresis followed by zymogram renaturing buffer monitored by staining and then quantifying the bands based on intensity.
RESULTS
We observed that P. g, F. n, and A. a were resistant to thyme extract at 0.2 µg/ml(MIC )and P.g and F. n showed no growth from 0.4 µg/ml to 100 µg/ml. Whereas, for MBC, A.a showed no growth at 100 µg/ml to 50 µg/ml .Thyme extract exhibited positive results with inhibition of 87.1% for antioxidant activity and 51% inhibition against MMP-9 as an anti-inflammatory effect.
CONCLUSION
Thyme extract has revealed significant antimicrobial, anti-inflammatory and antioxidant activity against A.a, P.gingivalis, and F.nucleatum. It can be acclaimed as an alternative to several traditional synthetic medicines to eradicate periodontal diseases.
Achieving High Accuracy in Lemon Quality Classification: A Comparative Study of Deep Learning and Transformer Models
Authors: Dümen Sezer , Yılmaz Esra Kavalcı , Adem Kemal , Avaroglu Erdinç 
Published: May 2023

Abstract
Agricultural product quality assessment is important for the efficiency and marketability of production. Quality assessment improves industry standards, increasing sales and reducing crop loss. Maintaining quality is of paramount importance for all processes, from production to sales. Artificial intelligence has recently been frequently used for product quality assessment in the agricultural field. Both in the literature and in practice, deep learning and machine learning methods are used to process images of agricultural products and evaluate their quality. They are classified according to specified standards. In this study, firstly, data augmentation operations were performed on the lemon dataset consisting of two classes, bad quality and good quality, by using rescaling, random zoom, random flip, and random rotation methods. Afterward, eight different deep-learning methods and two different transformer methods were used for classification. As a result of the study calculated the most successful result as 99.84% accuracy, 99.95% recall, and 99.66% precision with the ViT method. This value is the highest accuracy value in the literature. When the experimental results are evaluated, it shows that lemon classification processes are successfully performed using the ViT method.
Long-term recurrence interval of Vogt–Koyanagi–Harada disease post-COVID-19 vaccination: A case study
Authors: Muto Tetsuya , Sakamoto Masaaki , Imaizumi Shinichiro , Kamoi Koju 
Published: May 2023

Abstract
Purpose This study aimed to report a case of Vogt–Koyanagi–Harada (VKH) disease that recurred 46 years after initial treatment; the recurrence occurred 2 months after the third dose of coronavirus disease 2019 vaccination.Case report: A 59-year-old female patient had bilateral blurring for 2 months; she received her third dose of coronavirus disease 2019 vaccine 4 months before the onset of blurring. The best-corrected visual acuity was 1.0 in the right eye and 0.15 he left eye at the initial visit. Iritis and synechia between lens and iris were observed bilaterally. Sunset glow fundus was found in both eyes with no serous retinal detachments or disk hyperemia. She had a history of VKH disease and was treated with whole-body corticosteroid administration at another hospital when she was 13 years old. She was diagnosed with VKH disease recurrence, and oral corticosteroid intake and corticosteroid eyedrop treatments were initiated. The treatment response was good, but left synechia remained between the lens and iris in the left eye. Recurrence was not observed for 10 months until this study, and her best-corrected visual acuity was 1.0 in both eyes.Conclusion To our knowledge, this case represents the longest recorded interval of VKH disease recurrence in the literature.
Aplikasi Pupuk NPK Pada Tanaman Bawang Merah di Kabupaten Cirebon
Authors: Hamdani Kiki Kusyaeri , Susanto Heru , Nurawan Agus , Rodhian Saiful , Rahayu Sarwendah Puji 
Published: May 2023

Pemupukan merupakan pemberian hara diantaranya ke dalam tanah untuk mendukung pertumbuhan dan perkembangan tanaman bawang merah. Saat diaplikasikan, penggunaan pupuk NPK majemuk lebih praktis dibandingkan pupuk tunggal. Penelitian ini bertujuan untuk menguji pengaruh pupuk NPK majemuk terhadap hasil tanaman bawang merah di Kecamatan Pabuaran, Kabupaten Cirebon. Penelitian dilaksanakan di lahan sawah irigasi, Desa Pabuaran Lor, Kecamatan Pabuaran, Kabupaten Cirebon pada bulan Juni sampai September 2019. Penelitian menggunakan Rancangan Acak Kelompok Lengkap (RAKL) dengan sembilan perlakuan dan tiga ulangan. Setiap perlakuan terdiri dari dosis pupuk NPK (NPK 16-16-16 dan NPK 15-9-20) dan waktu aplikasinya. Data dianalisis dengan sidik ragam dengan uji lanjut Duncan Multiple Range Test pada taraf kepercayaan 95%. Hasil penelitian menunjukkan bahwa hasil bawang merah per hektar paling tinggi diperoleh pada perlakuan pupuk NPK 16-16-16 dengan dosis 650 kg ha-1 (350 kg ha-1 umur 0 HST dan 300 kg ha-1 umur 15 HST) dan NPK 15-9-20 dengan dosis 300 kg ha-1 (150 kg ha-1 umur 15 HST dan 150 kg ha-1 umur 35 HST) yaitu 22,60 ton ha-1 sedangkan bobot umbi per tanaman paling tinggi dihasilkan oleh perlakuan pupuk NPK 16-16-16 pada dosis 650 kg ha-1 (250 kg ha-1 umur 10 HST, 200 kg ha-1 umur 15 HST, dan 200 kg ha-1 umur 35 HST) dan NPK 15-9-20 pada dosis 200 kg ha-1 (100 kg ha-1 umur 25 HST dan 100 kg ha-1 umur 35 HST). terbaik diperoleh pada perlakuan pupuk NPK 16-16-16 dengan dosis 650 kg ha-1 (250 kg ha-1 umur 10 HST, 200 kg ha-1 umur 15 HST, dan 200 kg ha-1 umur 35 HST) dan NPK 15-9-20 pada dosis 200 kg ha-1 (100 kg ha-1 umur 25 HST dan 100 kg ha-1 umur 35 HST) dengan hasil yaitu bobot umbi per tanaman sebesar 68,67 g.
Karakterisasi Morfologi Pisang di Kabupaten Kampar Provinsi Riau
Authors: Sari Putri Lukmana , MS Zulkifli , Hardi Noer Arif , Akbar Daru 
Published: May 2023

Pisang (Musa spp.) merupakan buah paling penting di hampir setiap negara termasuk Indonesia. Pisang juga merupakan tanaman penting di dunia dan sumber pendapatan di banyak negara berkembang. Keanekaragaman pisang setiap daerahnya memiliki keunggulan masing-masing. Informasi terkait keragaman genetik tanaman sangat penting karena keragaman genetik yang tinggi merupakan salah satu kunci sukses dalam pengembangan tanaman. Terutama dalam penyediaan informasi pemuliaan tanaman. Bahan yang digunakan dalam penelitian ini sebanyak 6 aksesi pisang. Setiap spesies terdiri atas 3 individu yang diperoleh dari hasil eksplorasi di Kabupaten Kampar Provinsi Riau. Alat yang digunakan dalam penelitian ini antara lain adalah stapler, alat tulis, kertas label, kamera, dan penggaris. Pengamatan dan pertelaan morfologi mengikuti Musa Descriptor List. Deskripsi morfologi karakter kualitatif dari 6 populasi pisang telah berhasil didapatkan dan telah disajikan di dalam pembahasan. Hubungan kekerabatan berdasarkan penanda morfologi menggunakan 30 karakter kualitatif menunjukkan koefisien kemiripan genetiknya berkisar antara 0,28-0,60 yang terbagi dua yaitu Klaster A (A1; Pisang 40 Hari, Barangan Medan, Cavendish, Lilit, A2; Ambon) dan B (Pisang Kepok Taiwan). Perbedaan jenis pisang yang tumbuh menunjukkan pisang di Kabupaten Kampar memiliki keragaman morfologis yang cukup tinggi. Pengelompokan pisang pada penelitian ini dipengaruhi oleh persamaan karakter yang dimiliki oleh setiap aksesi yang pada akhirnya mengelompok berdasar persamaan spesiesnya. Dari penelitian ini, diharapkan dapat mengetahui keanekaragaman genetik dan hubungan kekerabatan pisang yang tumbuh di Kabupaten Kampar untuk nantinya akan didapatkan tetua pisang yang bagus sebagai bahan persilangan dan menghasilkan pisang yang potensial di masa mendatang.
El internado médico desde el enfoque de la salud basado en Derechos Humanos. Los casos de Costa Rica y México
Authors: Sancho - Ugalde Hilda - M , Mendoza - González Mauricio - F , Coto - Hernández Moisés , Vázquez - Martínez Francisco - D 
Published: May 2023

Se realiza una investigación desde el enfoque de la salud basado en derechos humanos, en el proceso de formación de estudiantil de Medicina a nivel del último año de la carrera. Es un estudio descriptivo, transversal, en una universidad pública mexicana y una privada costarricense, con una población de 532 internos, una encuesta anónima y voluntaria, integrada por 33 ítems [alfa de Cronbach=0,926]. En ambos países se denota la ausencia de asesoría y supervisión por parte del personal docente, así como jornadas superiores a las 80 horas semanales. Costa Rica y México comparten problemas de la educación médica durante el internado y tienen desafíos comunes en cuanto al impulso del derecho a la educación en las unidades médicas.
DESAIN PEMBELAJARAN KONTEKS SOSIAL-SOSIETAL DALAM PRAGMATIK DENGAN METODE KOOPERATIF TEKNIK INVESTIGASI
Authors: Rahardi Kunjana 
Published: May 2023

AbstractÂ Pragmatic learning must be research based. By basing on research, pragmatic learning in language majors and language education will be of high quality. Until now, pragmatic learning is still based on the views of Western experts. On the basis of that concern, the authors sought to examine specific culture-based pragmatics related to the socio-societal context. The results of the study are utilized in pragmatic learning. The main objective of this study is to describe the design of social-societal context learning models in pragmatics. The paradigm that was followed was the Ignasian-based reflective learning paradigm. The learning method applied is the cooperative learning method. The technique applied is a group investigation technique. Furthermore, this research is useful in the following matters: (a) improving and improving the quality of pragmatic learning in tertiary institutions; (b) produce researchers in the field of reliable pragmatics because they are used to wrestling with pragmatic research; (c) developing pragmatics, especially pragmatics based on specific culture.Keywords: Group investigation technique; cooperative learning method; reflective teachingÂ Pembelajaran pragmatik harus berbasis riset. Dengan mendasarkan pada riset, pembelajaran pragmatik di jurusan-jurusan bahasa dan pendidikan bahasa akan berkualitas. Hingga sekarang, pembelajaran pragmatik masih didasarkan pada pandangan pakar Barat. Atas dasar keprihatinan itu, penulis berusaha meneliti pragmatik berbasis kultur spesifik terkait konteks sosial-sosietal. Hasil kajian tersebut dimanfaatkan dalam pembelajaran pragmatik. Tujuan utama penelitian ini adalah menggambarkan desain model pembelajaran konteks sosial-sosietal dalam pragmatik. Paradigma yang diikuti adalah paradigma pembelajaran reflektif berbasis Ignasian. Metode pembelajaran yang diterapkan adalah metode pembelajaran kooperatif. Adapun teknik yang diterapkan adalah teknik investigasi kelompok (grup-investigation technique). Selanjutnya, penelitian ini bermanfaat dalam hal-hal berikut: (a) memperbaiki dan meningkatkan kualitas pembelajaran pragmatik di perguruan tinggi; (b) menghasilkan periset-periset bidang pragmatik yang andal karena mereka sudah terbiasa bergulat dengan riset pragmatik; (c) mengembangkan ilmu pragmatik khususnya pragmatik yang berbasis kultur spesifik.Â Kata Kunci: Group investigation technique; cooperative learning method; pembelajaran reflektifÂ
UPAYA MENINGKATKAN HASIL BELAJAR IPAS MELALUI MODEL PROBLEM BASED LEARNING MATERI WUJUD ZAT DAN PERUBAHANNYA
Authors: Asendra Marcesa Dita , Samsiyah Nur , Arif Hidayati Yuyun 
Published: May 2023

Abstract
The purpose of this study was to evaluate the development of student learning outcomes, teacher and student activities, the teacher's ability to direct learning and student responses to the application of problem based learning (PBL) in the learning process. This type of research is Classroom Action Research (CAR). The subjects of this study were class IV students of SD Negeri Bagi 01 for the academic year 2022/2023, which consisted of 5 students. In this study, observation sheets, tests and questionnaires were used as data collection tools which were analyzed using descriptive statistics. The results showed that (1) the percentage of individual total perfection increased from Cycle I to Cycle II, namely 69% to 94%, as well as the percentage of total classical perfection increased from 50% to 80%. (2) The activity of teachers and students increases during the learning process. (3) the teacher's learning management skills increased from the "adequate" category to the "good" category. (4) Student responses were generally positive, with 100% of students saying they enjoyed learning using this constructive problem-based learning (PBL) model. From this study it can be concluded that the application of the Problem Based Learning (PBL) learning model can improve the science learning outcomes of fourth grade students at SD Negeri Bagi 01 regarding the material form of matter and its changes.
Keywords: Problem Based Learning (PBL), Learning Results
PENERAPAN PROBLEM BASED LEARNING UNTUK MENINGKATAN MOTIVASI DAN AKTIVITAS BELAJAR SISWA KELAS V SD NEGERI 3 PANDAK
Authors: Wulandari Etika Rian , Listiani Ivayuni , Khotijah Siti 
Published: May 2023

This research is a classroom action research that aims to increase motivation and learning activities in science lessons for fifth grade students at SDN 3 Pandak using the Problem Based Learning model. Based on the results of the application using the Problem Based Learning learning model in science learning, it shows an increase in student motivation and activity during the learning process. It can be seen from the increase that occurs in each learning cycle. In cycle I, the motivation of students with an average of 66,4% increased in cycle II with an average of 86.7%, and the activities of students in cycle I with an average of 59,37% increased in cycle II with an average of 91 .14%. This proves that the use of the Problem Based Learning can increase the motivation and activity of students in the learning process