bluuebunny/crossref_metadata_2025_split huggingface dataset:
Transcriptomic and metabolomic analysis reveals that symbiotic nitrogen fixation enhances drought resistance in common bean
Authors: López Cristina Mª , Alseekh Saleh , Torralbo Fernando , Martínez Rivas Félix J , Fernie Alisdair R , Amil-Ruiz Francisco , Alamillo Josefa M 
Published: March 2023

Abstract
Common bean (Phaseolus vulgaris L.), one of the most important legume crops, uses atmospheric nitrogen through symbiosis with soil rhizobia, reducing the need for nitrogen fertilization. However, this legume is particularly sensitive to drought conditions, prevalent in arid regions where this crop is cultured. Therefore, studying the response to drought is important to sustain crop productivity. We have used integrated transcriptomic and metabolomic analysis to understand the molecular responses to water deficit in a marker-class common bean accession cultivated under N2 fixation or fertilized with nitrate (NO3–). RNA-seq revealed more transcriptional changes in the plants fertilized with NO3– than in the N2-fixing plants. However, changes in N2-fixing plants were more associated with drought tolerance than in those fertilized with NO3–. N2-fixing plants accumulated more ureides in response to drought, and GC/MS and LC/MS analysis of primary and secondary metabolite profiles revealed that N2-fixing plants also had higher levels of abscisic acid, proline, raffinose, amino acids, sphingolipids, and triacylglycerols than those fertilized with NO3–. Moreover, plants grown under nitrogen fixation recovered from drought better than plants fertilized with NO3–. Altogether we show that common bean plants grown under symbiotic nitrogen fixation were more protected against drought than the plants fertilized with nitrate.
LONG HYPOCOTYL IN FAR-RED 1 mediates a trade-off between growth and defence under shade in Arabidopsis
Authors: Sng Benny Jian Rong , Vu Kien Van , Choi Ian Kin Yuen , Chin Hui Jun , Jang In-Cheol 
Published: March 2023

AbstractPlants respond to vegetative shade with developmental and physiological changes that are collectively known as shade avoidance syndrome (SAS). Although LONG HYPOCOTYL IN FAR-RED 1 (HFR1) is known to be a negative regulator of SAS by forming heterodimers with other basic helix-loop-helix (bHLH) transcription factors to inhibit them, its function in genome-wide transcriptional regulation has not been fully elucidated. Here, we performed RNA-sequencing analyses of Arabidopsis thaliana hfr1-5 mutant and HFR1 overexpression line [HFR1(ΔN)-OE] to comprehensively identify HFR1-regulated genes at different time points of shade treatment. We found that HFR1 mediates the trade-off between shade-induced growth and shade-repressed defence, by regulating the expression of relevant genes in the shade. Genes involved in promoting growth, such as auxin biosynthesis, transport, signalling and response were induced by shade but suppressed by HFR1 under both short and long durations of shade. Likewise, most ethylene-related genes were shade-induced and HFR1-repressed. However, shade suppressed defence-related genes, while HFR1 induced their expression, especially under long durations of shade treatment. We demonstrated that HFR1 confers increased resistance to bacterial infection under shade.
Wigner Distribution Deconvolution Adaptation for Live Ptychography Reconstruction
Authors: Bangun Arya , Baumeister Paul F , Clausen Alexander , Weber Dieter , Dunin-Borkowski Rafal E 
Published: March 2023

Abstract
We propose a modification of Wigner distribution deconvolution (WDD) to support live processing ptychography. Live processing allows to reconstruct and display the specimen transmission function gradually while diffraction patterns are acquired. For this purpose, we reformulate WDD and apply a dimensionality reduction technique that reduces memory consumption and increases processing speed. We show numerically that this approach maintains the reconstruction quality of specimen transfer functions as well as reduces computational complexity during acquisition processes. Although we only present the reconstruction for scanning transmission electron microscopy datasets, in general, the live processing algorithm we present in this paper can be applied to real-time ptychographic reconstruction for different fields of application.
Weight Changes in Young Service Members After Lower Limb Amputation: Insights From Group-Based Trajectory Modeling
Authors: Gunterstockman Brittney Mazzone , Russell Esposito Elizabeth , Yoder Adam , Smith Clair , Farrokhi Shawn 
Published: March 2023

ABSTRACT

Introduction
Weight gain can contribute to osteoarthritis, cardiovascular disease, low back pain, and poor health-related quality of life. Weight trajectory patterns have been described in older veterans with limb loss; however, there is limited evidence of changes in weight in younger veterans with limb loss.

Materials and Methods
Service members with unilateral or bilateral lower limb amputations (LLAs) (and without an upper limb amputation) were included in this retrospective cohort analysis (n = 931). The mean postamputation baseline weight was 78.0 ± 14.1 kg. Bodyweight and sociodemographic data were extracted from clinical encounters within electronic health records. Group-based trajectory modeling assessed weight change patterns 2 years postamputation.

Results
Three weight change trajectory groups were identified: 58% (542 of 931) of the cohort had stable weight, 38% (352 of 931) had weight gain (mean gain = 19.1 kg), and 4% (31 of 931) had weight loss (mean loss = 14.5 kg). Individuals with bilateral amputations were more frequently noted in the weight loss group compared with those with unilateral amputations. Individuals with LLAs because of trauma other than blasts were more frequently found in the stable weight group compared with those with amputations because of disease or a blast. Younger individuals (&lt;20 years old) with amputations were more likely to be in the weight gain group compared with older individuals.

Conclusions
More than half the cohort maintained a stable weight for two years after amputation, and more than a third experienced weight gain during the same time frame. Knowledge of underlying factors that were associated with weight gain could inform preventative strategies for young individuals with LLAs.
A UV census of the environments of stripped-envelope supernovae
Authors: Sun Ning-Chen , Maund Justyn R , Crowther Paul A 
Published: March 2023

ABSTRACTThis paper reports an environmental analysis of 41 uniformly selected stripped-envelope supernovae (SESNe) based on deep ultraviolet–optical images acquired by the Hubble Space Telescope. Young stellar populations are detected in most SN environments and their ages are derived with a hierarchical Bayesian approach. The age distributions are indistinguishable between Type IIb and Type Ib, while that for Type Ic is systematically younger. This suggests that the Type Ic SN progenitors are more massive, while the Type IIb and Type Ib SNe have very similar progenitor masses. Our result supports a hybrid envelope-stripping mechanism, in which the hydrogen envelopes of the SESN progenitors are stripped via a mass-insensitive process (e.g. binary interaction), while the helium envelopes are stripped via a mass-sensitive process (e.g. stellar wind of the post-binary interaction progenitor). We also provide progenitor constraints for three Type Ibn SNe and two broad-lined Type Ic SNe. All these results demonstrate the importance of the very diverse mass-loss processes in the origins of SESNe.
Investigation of genotoxic effect of octyl gallate used as an antioxidant food additive in in vitro test systems
Authors: Avuloglu Yilmaz Ece , Yuzbasioglu Deniz , Unal Fatma 
Published: March 2023

Abstract
Several antioxidant food additives are added to oils, soups, sauces, chewing gum, potato chips, and so on. One of them is octyl gallate. The purpose of this study was to evaluate the potential genotoxicity of octyl gallate in human lymphocytes, using in vitro chromosomal abnormalities (CA), sister chromatid exchange (SCE), cytokinesis block micronucleus cytome (CBMN-Cyt), micronucleus-FISH (MN-FISH), and comet tests. Different concentrations (0.031, 0.063, 0.125, 0.25, and 0.50 μg/ml) of octyl gallate were used. A negative (distilled water), a positive (0.20 μg/ml Mitomycin-C), and a solvent control (8.77 μl/ml ethanol) were also applied for each treatment. Octyl gallate did not cause changes in chromosomal abnormalities, micronucleus, nuclear bud (NBUD), and nucleoplasmic bridge (NPB) frequency. Similarly, there was no significant difference in DNA damage (comet assay), percentage of centromere positive and negative cells (MN-FISH test) compared to the solvent control. Moreover, octyl gallate did not affect replication and nuclear division index. On the other hand, it significantly increased the SCE/cell ratio in three highest concentrations compared to solvent control at 24 h treatment. Similarly, at 48 h treatment, the frequency of SCE raised significantly compared to solvent controls at all the concentrations (except 0.031 μg/ml). An important reduction was detected in mitotic index values in the highest concentration at 24 h treatment and almost all concentrations (except 0.031 and 0.063 µg/ml) at 48 h treatment. The results obtained suggest that octyl gallate has no important genotoxicological action on human peripheral lymphocytes at the concentrations applied in this study.
Dynamic interplay between RPL3- and RPL3L-containing ribosomes modulates mitochondrial activity in the mammalian heart
Authors: Milenkovic Ivan , Santos Vieira Helaine Graziele , Lucas Morghan C , Ruiz-Orera Jorge , Patone Giannino , Kesteven Scott , Wu Jianxin , Feneley Michael , Espadas Guadalupe , Sabidó Eduard , Hübner Norbert , van Heesch Sebastiaan , Völkers Mirko , Novoa Eva Maria 
Published: March 2023

Abstract
The existence of naturally occurring ribosome heterogeneity is now a well-acknowledged phenomenon. However, whether this heterogeneity leads to functionally diverse ‘specialized ribosomes’ is still a controversial topic. Here, we explore the biological function of RPL3L (uL3L), a ribosomal protein (RP) paralogue of RPL3 (uL3) that is exclusively expressed in skeletal muscle and heart tissues, by generating a viable homozygous Rpl3l knockout mouse strain. We identify a rescue mechanism in which, upon RPL3L depletion, RPL3 becomes up-regulated, yielding RPL3-containing ribosomes instead of RPL3L-containing ribosomes that are typically found in cardiomyocytes. Using both ribosome profiling (Ribo-seq) and a novel orthogonal approach consisting of ribosome pulldown coupled to nanopore sequencing (Nano-TRAP), we find that RPL3L modulates neither translational efficiency nor ribosome affinity towards a specific subset of transcripts. In contrast, we show that depletion of RPL3L leads to increased ribosome–mitochondria interactions in cardiomyocytes, which is accompanied by a significant increase in ATP levels, potentially as a result of fine-tuning of mitochondrial activity. Our results demonstrate that the existence of tissue-specific RP paralogues does not necessarily lead to enhanced translation of specific transcripts or modulation of translational output. Instead, we reveal a complex cellular scenario in which RPL3L modulates the expression of RPL3, which in turn affects ribosomal subcellular localization and, ultimately, mitochondrial activity.
A Practical Guide to Relugolix: Early Experience With Oral Androgen Deprivation Therapy
Authors: Kasparian Saro , Wei Oren , Tsai Ni-Chun , Palmer Joycelynne , Pal Sumanta , Lyou Yung , Dorff Tanya 
Published: March 2023

Abstract

Background
Relugolix is the newest form of androgen deprivation therapy (ADT) approved for prostate cancer. However, as an oral drug, several real-world concerns exist, particularly medication compliance, safety with other androgen receptor-targeted agents, and financial burden to patients.

Methods
A single institution retrospective chart review was conducted evaluating all patients who were prescribed relugolix for any prostate cancer indication from January 1, 2021 to January 31, 2022. Demographic data, cardiac risk factors, concomitant therapy usage, and PSA/testosterone levels, were abstracted from the chart review. Adverse effects were obtained by examining progress notes. Compliance was assessed by clinic notes as well as prescription fills by specialty pharmacy records. The reasons patients did not fill or discontinued the medication were noted.

Results
Hundred and one patients were prescribed relugolix, and 91 patients consented to research. Seventy-one (78%) patients filled the prescription to relugolix, with a median follow-up of 5 months. Prescription fill data were available for 45 (63%) patients, with 94% of days covered. The most commonly reported reason not to fill was cost at 50%. Sixty-six (93%) patients reported never missing a dose. PSA levels were available in 71 (100%) patients with 69 (97%) showing stable or improved PSA. Testosterone levels were available in 61 (86%) of patients, which showed 61 (100%) stable or successful castration. Twenty-four (34%) patients used relugolix in combination. No new major safety signals were seen in combination therapy. Nineteen (27%) patients had switched to another form of ADT. Fifteen of these (79%) felt similar or better on relugolix therapy.

Conclusions
Compliance with relugolix seemed acceptable. No major new safety signals were seen, even in combination. Among patients who switched therapy, most tolerated relugolix similarly or better than the previous form of ADT. The cost was a major reason for patients not initiating and for discontinuing therapy.
Reciprocal regulation of flower induction byELF3αandELF3βgenerated via alternative promoter usage
Authors: Wang Peng , Li Yu , Liu Zhe , Li Xuhan , Wang Yicheng , Liu Weijuan , Li Xiao , Hu Jianjian , Zhu Wenyi , Wang Changquan , Li Shan , Gu Tingting , Xu Dongqing , Tang Chao , Wang Yingtao , Li Chao , Zhang Shaoling , Wu Juyou 
Published: March 2023

AbstractFlowering is critical for sexual reproduction and fruit production. Several pear (Pyrus sp.) varieties produce few flower buds, but the underlying mechanisms are unknown. The circadian clock regulator EARLY FLOWERING3 (ELF3) serves as a scaffold protein in the evening complex that controls flowering. Here, we report that the absence of a 58-bp sequence in the 2nd intron of PbELF3 is genetically associated with the production of fewer flower buds in pear. From rapid amplification of cDNA ends sequencing results, we identified a short, previously unknown transcript from the PbELF3 locus, which we termed PbELF3β, whose transcript level was significantly lower in pear cultivars that lacked the 58-bp region. The heterologous expression of PbELF3β in Arabidopsis (Arabidopsis thaliana) accelerated flowering, whereas the heterologous expression of the full-length transcript PbELF3α caused late flowering. Notably, ELF3β was functionally conserved in other plants. Deletion of the 2nd intron reduced AtELF3β expression and caused delayed flowering time in Arabidopsis. AtELF3β physically interacted with AtELF3α, disrupting the formation of the evening complex and consequently releasing its repression of flower induction genes such as GIGANTEA (GI). AtELF3β had no effect in the absence of AtELF3α, supporting the idea that AtELF3β promotes flower induction by blocking AtELF3α function. Our findings show that alternative promoter usage at the ELF3 locus allows plants to fine-tune flower induction.
Exploring the Relationship between Federal Reform and the Representation of the People: The Case of the Belgian House of Representatives
Authors: Frateur Jakob , Bursens Peter , Meier Petra 
Published: March 2023

Abstract
Federal systems tend to have two venues of representation to ensure that both the people as a whole and the constituent units are represented at the federal level. While this double representation is put forward as a basic (normative) feature of federal systems, little to no empirical research has been conducted on this issue. This contribution therefore studies the representation of the people as a whole and of the constituent units in the Belgian House of Representatives by means of a representative claims analysis of 4,757 oral parliamentary questions. As federal systems tend to be dynamic, the analysis is based on six periods of federal reform through which Belgium decentralized. Our findings show that, over time, the representation of the constituent units increased and exceeded the representation of the people, providing unique empirical input for the debate about the idea that federalism is by definition beneficial for democracy. We problematize our results from a democratic point of view, as—despite being able to vote—the people as a whole are hardly any more represented by the House. Going beyond the Belgian case, we argue that processes of federalization should address the adequate representation of both the constituent units and the people.
State Attorneys General and their Challenges to Federal Policies: Insights from the Texas v. California Litigation Regarding the Affordable Care Act
Authors: Dichio Michael A , Singer Phillip 
Published: March 2023

Abstract
This article explains a rising trend in American politics: the increasingly prominent role the state attorneys general (AGs) play in challenging federal policies. It focuses on one particularly important case—Republican efforts to overturn the Affordable Care Act in Texas v. California. We consider how state AGs and solicitors general (SGs) drive policy efforts through litigation and the factors that contribute to their participation. We find, first, that although members of the out-of-power party in Washington are the ones who typically bring state lawsuits, the Texas litigation demonstrates that these lawsuits are also a vehicle by which members of the party in power try to achieve goals that are otherwise unattainable through the legislative process. Second, Republican AGs submerge partisan arguments in “constitution-talk” with the aim of achieving a policy goal that was defeated democratically. We reach these conclusions through content analysis of AG press releases and semi-structured interviews with litigators and other elite actors.
Early career academic's odyssey: A narrative study of her professional identity construction
Authors: Mula-Falcón Javier , Caballero Katia 
Published: March 2023

Abstract
Recent decades have seen a major transformation of the Spanish university system caused by changes introduced in the teaching staff evaluation procedure in which research has been prioritized. As a result, there has been a growing interest in studying how these procedures impact the way in which early career academics construct their professional identity. In this vein, this article aims to analyze how the professional identity of this group is constructed and developed in the current context of higher education. To this end, a single case study was conducted, applying a biographical-narrative approach. The results show how professional identity development has become polarized in terms of initial rejection to full acceptance of the demands of modern academia. Consequently, professional practices have also been affected by the prioritization of research over teaching, leading to the development of unethical practices to maintain competitiveness. All of these effects have contributed to high levels of stress and job dissatisfaction. Furthermore, maternity is also seen as an obstacle to female academics’ professional and academic development. Finally, our findings highlight the need for policies that provide full support aimed at helping young researchers live balanced professional lives.
Scarcity mindset reduces empathic responses to others’ pain: the behavioral and neural evidence
Authors: Li Wanchen , Meng Jing , Cui Fang 
Published: March 2023

AbstractResource scarcity pervades our life. A scarcity mindset triggered by perceiving insufficient resources has been proven to influence our cognition and behaviors, yet it remains unknown whether this mindset specifically influences empathy. The present study induced feelings of scarcity or abundance in separate groups of participants through experimental manipulation and examined the effects of both mindsets on the behavioral and neural responses to others’ pain. Behaviorally, pain intensity ratings of others’ pain were lower in the scarcity group than in the abundance group. The analysis of event-related potentials revealed that N1 amplitudes for painful and nonpainful stimuli were comparable in the scarcity group but differed significantly in the abundance group. Additionally, while both groups showed larger late positive potential amplitudes for painful stimuli than for nonpainful stimuli, this amplitude differential was significantly smaller in the scarcity group than in the abundance group. Thus, behavioral and neural evidence suggests that inducing a scarcity mindset significantly dampens the ability to empathize with others’ pain during both the early and late stages of empathic processing. These findings shed light on our understanding of how a scarcity mindset may influence social emotions and behaviors.
Green electrosynthesis of drug metabolites
Authors: Asra Ridho , Jones Alan M 
Published: March 2023

Abstract
In this concise review, the field of electrosynthesis (ES) as a green methodology for understanding drug metabolites linked to toxicology is exemplified. ES describes the synthesis of chemical compounds in an electrochemical cell. Compared to a conventional chemical reaction, ES operates under green conditions (the electron is the reagent) and has several industrial applications, including the synthesis of drug metabolites for toxicology testing. Understanding which circulating drug metabolites are formed in the body is a crucial stage in the development of new medicines and gives insight into any potential toxic pathologies resulting from the metabolites formed. Current methods to prepare drug metabolites directly from the drug molecule often involve time-consuming multistep syntheses. Throughout this review, the application of green ES to (i) identify drug metabolites, (ii) enable their efficient synthesis, and (iii) investigate the toxicity of the metabolites generated are highlighted.