bluuebunny/crossref_metadata_2025_split huggingface dataset:
The effect of the leisure activities based on chess and cards for improving cognition of older adults: study protocol for a cluster randomized controlled trial
Authors: Shi Xiaojuan , wang Yanrong , Wu Yueping , Li Jiangping 
Published: July 2023

Abstract
Background
With the increase in age, the probability of cognitive impairment in the older people is increasing. More and more evidence shows that participating in leisure activities, especially chess and cards, is beneficial to the cognition and mental state of the older people. But the evidence for causal inference is limited. There is a need to conduct a fully powered randomized controlled trial (RCT) and long-term follow-up to test the effectiveness of intervention measures in improving cognitive function and mental state. This paper describes the methodology of a cluster RCT designed to determine the effect of chess and cards leisure activities on the cognitive function of the older people over 60 years old.

Methods/design
A cluster RCT consisting of 8 clusters will be conducted in four regions of Ningxia, China (Helan, Litong, Qingtongxia, and Shapotou). Clusters will be randomly assigned to the advocacy intervention group, which is also delivered with free leisure activities tools (chess and cards), or the control group. A baseline survey will be conducted before the intervention. A mid-term and final survey will be carried out twelve and twenty-four months after the intervention, respectively. The primary outcome is a change in cognitive function, and the secondary outcomes are changes in anxiety, depression, and loneliness.

Discussion
The results of this RCT will be helpful to (1) confirm the effectiveness of chess and cards leisure activities in improving the cognitive function of the older people over 60 years old; (2) determine the relationship between the frequency and duration of chess and cards leisure activities and cognitive function; (3) provide evidence of promoting participation in leisure activities through education campaigns and free provision of chess and cards tools; and (4) provide valuable information for successful aging.

Trial registration
Chinese Clinical Trial Registry: ChiCTR2200066817. Registered on 19 December 2022.
Characterization of protein cargo of Echinococcus granulosus extracellular vesicles in drug response and its influence on immune response
Authors: Nicolao María Celeste , Rodrigues Christian Rodriguez , Coccimiglio Magalí B. , Ledo Camila , Docena Guillermo H. , Cumino Andrea C. 
Published: July 2023

Abstract
Background
The Echinococcus granulosus sensu lato species complex causes cystic echinococcosis, a zoonotic disease of medical importance. Parasite-derived small extracellular vesicles (sEVs) are involved in the interaction with hosts intervening in signal transduction related to parasite proliferation and disease pathogenesis. Although the characteristics of sEVs from E. granulosus protoscoleces and their interaction with host dendritic cells (DCs) have been described, the effect of sEVs recovered during parasite pharmacological treatment on the immune response remains unexplored.

Methods
Here, we isolated and characterized sEVs from control and drug-treated protoscoleces by ultracentrifugation, transmission electron microscopy, dynamic light scattering, and proteomic analysis. In addition, we evaluated the cytokine response profile induced in murine bone marrow-derived dendritic cells (BMDCs) by qPCR.

Results
The isolated sEVs, with conventional size between 50 and 200 nm, regardless of drug treatment, showed more than 500 cargo proteins and, importantly, 20 known antigens and 70 potential antigenic proteins, and several integral-transmembrane and soluble proteins mainly associated with signal transduction, immunomodulation, scaffolding factors, extracellular matrix-anchoring, and lipid transport. The identity and abundance of proteins in the sEV-cargo from metformin- and albendazole sulfoxide (ABZSO)-treated parasites were determined by proteomic analysis, detecting 107 and eight exclusive proteins, respectively, which include proteins related to the mechanisms of drug action. We also determined that the interaction of murine BMDCs with sEVs derived from control parasites and those treated with ABZSO and metformin increased the expression of pro-inflammatory cytokines such as IL-12 compared to control cells. Additionally, protoscolex-derived vesicles from metformin treatments induced the production of IL-6, TNF-α, and IL-10. However, the expression of IL-23 and TGF-β was downregulated.

Conclusions
We demonstrated that sEV-cargo derived from drug-treated E. granulosus protoscoleces have immunomodulatory functions, as they enhance DC activation towards a type 1 pro-inflammatory profile against the parasite, and therefore support the proposal of a new approach for the prevention and treatment of secondary echinococcosis.

Graphical abstract
Trimethoprim-sulfamethoxazole prophylaxis during treatment of granulomatosis with polyangiitis with rituximab in the United States of America: a retrospective cohort study
Authors: Mendel Arielle , Behlouli Hassan , de Moura Cristiano Soares , Vinet Évelyne , Curtis Jeffrey R. , Bernatsky Sasha 
Published: July 2023

Abstract
Background
Antibiotic prophylaxis is recommended during ANCA-associated vasculitis (AAV) induction. We aimed to describe the frequency, persistence, and factors associated with trimethoprim-sulfamethoxazole (TMP-SMX) use in an adult population sample with granulomatosis with polyangiitis (GPA) treated with rituximab (RTX).

Methods
We identified adults with GPA treated with RTX within the Merative™ Marketscan® Research Databases (2011–2020). TMP-SMX prophylaxis was defined as a $$\ge$$
≥
28-day prescription dispensed within a month of starting RTX. We estimated TMP-SMX persistence, allowing prescription refill gaps of 30 days. Multivariable logistic regression and Cox proportional hazards regression assessed the factors associated with baseline TMP-SMX use and persistence, respectively. Covariates included age, sex, calendar year, insurance type, immunosuppressant use, hospitalization, and co-morbidities.

Results
Among 1877 RTX-treated GPA patients, the mean age was 50.9, and 54% were female. A minority (n = 426, 23%) received TMP-SMX with a median persistence of 141 (IQR 83–248) days. In multivariable analyses, prophylaxis was associated with prednisone use in the month prior to RTX ($$\ge$$
≥
20 mg/day vs none, OR 3.96; 95% CI 3.0–5.2; 1–19 mg/day vs none, OR 2.63; 95% CI 1.8–3.8), and methotrexate use (OR 1.48, 95% CI 1.04–2.1), intensive care (OR 1.95; 95% CI 1.4–2.7), and non-intensive care hospitalization (OR 1.56; 95% CI 1.2–2.1) in the 6 months prior to RTX. Female sex (OR 0.63; 95% CI 0.5–0.8) was negatively associated with TMP-SMX use.

Conclusions
TMP-SMX was dispensed to a minority of RTX-treated GPA patients, more often to those on glucocorticoids and with recent hospitalization. Further research is needed to determine the optimal use and duration of TMP-SMX prophylaxis following RTX in AAV.
Parental perceptions of a novel subsidy program to address the financial burden of milk allergy: a qualitative study
Authors: Bhamra Manvir , Harbottle Zoe , Golding Michael A , Ben-Shoshan Moshe , Roos Leslie E , Abrams Elissa M , Penner Sara J , St-Vincent Jo-Anne , Protudjer Jennifer LP 
Published: July 2023

Abstract
Background
Approximately 6–7% of Canadian children have food allergy. These families face substantial burdens due to the additional costs incurred purchasing allergy-friendly products necessary for management compared to families without food allergies. In the year prior to the COVID-19 pandemic, these costs were equivalent to an average of $200 monthly compared to families without food allergy. As food prices continue to rise, rates of food insecurity also increase, disproportionately affecting households with food allergy who have limited choices at food banks.

Methods
Families living or working in Winnipeg, Canada with an annual net income of about $70,000 or less the year prior to recruitment and a child under the age of 6 years old with a physician diagnosed milk allergy were recruited between January and February 2022. Participating families received bi-weekly home deliveries for six months, from March to August 2022, of subsidy kits containing ~$50 worth of milk allergy-friendly products. Semi-structured interviews, completed ± 2 weeks from the final delivery, were audio-recorded, transcribed verbatim, and analyzed thematically.

Results
Eight interviews, averaging 32 min (range 22–54 min), were completed with mothers from all different families. On average, mothers were 29.88 ± 4.39 years old and children were 2.06 ± 1.32 years old. All children reported allergies in addition to milk. Based on the data from these interviews, we identified 3 themes: food allergy causes substantial burden for families, “I have to get his allergy-friendly food first before getting to my basic needs”, and perceived emotional and financial benefits of a milk allergy-friendly food subsidy program.

Conclusions
This study, along with previous research, suggests that there is a need for assistance for families managing milk allergies. It also provides important information to inform development of programs which can address these financial challenges. Our in-kind food subsidy was perceived as having a positive impact on food costs and stress associated with food allergy management, however, parents identified a need for more variety in the food packages. Future programs should strive to incorporate a greater variety of products to address this limitation.
Autoimmune gastritis as an unexpected cause of diarrhea in a young adult with type I diabetes: a case report
Authors: Meyers Taylor M. , Reeves Patrick T. , Lombardo Jamie L. , Anisowicz Sarah K. , Larson Noelle S. , Rogers Philip L. 
Published: July 2023

Abstract
Background
Type 1 diabetes mellitus (T1DM) is a lifelong diagnosis that involves immune-mediated damage of pancreatic beta cells and subsequent hyperglycemia, manifesting as: polyuria, polydipsia, polyphagia, and weight loss. Treatment of type 1 diabetes centers on insulin administration to replace or supplement the body's own insulin with the goal of achieving euglycemia and preventing or minimizing complications. Patients with T1DM are at risk for developing other autoimmune conditions, most commonly thyroid or celiac disease.

Case presentation
A 20-year-old African American female with T1DM was referred by her endocrinologist to pediatric gastroenterology for 2 months of nocturnal, non-bloody diarrhea, left lower quadrant pain, and nausea; she was also being followed by neurology for complaints of lower extremity paresthesias and pain. The patient’s initial lab-workup was remarkable for a low total Immunoglobulin A (IgA) level of < 6.7 mg/dL. As IgA deficiency is associated with an increased risk of celiac disease, the patient underwent upper and lower endoscopy, which was grossly unremarkable; however, histology revealed a pattern consistent with autoimmune gastritis. Subsequent serum evaluation was remarkable for an elevated fasting gastrin level and an elevated parietal cell antibody level without macrocytic anemia, iron deficiency, or vitamin B12 depletion. The patient was diagnosed with autoimmune gastritis (AIG) and subsequently initiated on parenteral B12 supplementation therapy with improvement in her neurologic and gastrointestinal symptoms.

Conclusion
This case illustrates the importance of recognition of red flag findings in a patient with known autoimmune disease. Following well-established health maintenance recommendations for individuals with T1DM ensures that common comorbidities will be detected. Autoimmune gastritis, while a rarer pathology in the pediatric population, deserves consideration in patients with pre-existing autoimmune conditions and new gastrointestinal or neurologic symptoms, as AIG can be associated with poor outcomes and risk of malignancy. Initial lab findings associated with an eventual diagnosis of AIG typically include anemia, iron deficiency, or Vitamin B12 deficiency. However, as demonstrated in this case, symptoms of AIG can rarely present before anemia or Vitamin B12 deficiency develops. To prevent permanent neurological damage, parenteral Vitamin B12 therapy must be considered even in the absence of Vitamin B12 deficiency, especially in those patients already experiencing neurological symptoms.
Tolvaptan for water retention in heart failure: a systematic review
Authors: Pan Yujing , Li Haoyang , Gao Jin , Mi Zishuo , Chen Hao , Li Ying 
Published: July 2023

Abstract
Objective
The purpose of this systematic review is to collect, appraise, and synthesize existing evidence from systematic reviews and meta-analyses (SRs/MAs) on the effectiveness of tolvaptan for water retention in heart failure.

Methods
A comprehensive literature search was performed on PubMed, EMBASE, web of science, Cochrane reviews for SRs/Mas published between the databases’ establishment to November 17, 2021. All the records were managed with Endnote 20. Standardized forms were used to extract data. Revman 5.3 was used to make forest plots to show the characteristics of outcomes. The methodological and evidence quality were respectively evaluated by AMSTAR-2 (A MeaSurement Tool to Assess systematic Reviews 2) and GRADE (Grading of Recommendation of Assessment, Development, and Evaluation) system.

Results
A total of 9 SRs/Mas between 2015 to 2020 met inclusion criteria. Serum sodium concentration and urine output were considered as primary outcomes and body weight change and all-cause mortality as second outcomes. Through conducting forest plots, it appeared that tolvaptan brought more positive effect than conventional therapies. It was pessimistic when it comes to the quality of the 9 studies. all the 9 articles were rated as low-quality because AMSTAR 2 evaluation showed that they each had at least one critical item (items 2, 4, 7, 9, 11, 13 and 15) defect. Besides, every article had a few non-critical item defects too. The result of GRADE assessment was not optimistic, so the overall quality of the evidences was low as well.

Conclusion
Tolvaptan can be recommended for water retention in HF patients, but more evidence is needed.
Resistome expansion in disease-associated human gut microbiomes
Authors: Fredriksen Simen , de Warle Stef , van Baarlen Peter , Boekhorst Jos , Wells Jerry M. 
Published: July 2023

Abstract
Background
The resistome, the collection of antibiotic resistance genes (ARGs) in a microbiome, is increasingly recognised as relevant to the development of clinically relevant antibiotic resistance. Many metagenomic studies have reported resistome differences between groups, often in connection with disease and/or antibiotic treatment. However, the consistency of resistome associations with antibiotic- and non-antibiotic–treated diseases has not been established. In this study, we re-analysed human gut microbiome data from 26 case-control studies to assess the link between disease and the resistome.

Results
The human gut resistome is highly variable between individuals both within and between studies, but may also vary significantly between case and control groups even in the absence of large taxonomic differences. We found that for diseases commonly treated with antibiotics, namely cystic fibrosis and diarrhoea, patient microbiomes had significantly elevated ARG abundances compared to controls. Disease-associated resistome expansion was found even when ARG abundance was high in controls, suggesting ongoing and additive ARG acquisition in disease-associated strains. We also found a trend for increased ARG abundance in cases from some studies on diseases that are not treated with antibiotics, such as colorectal cancer.

Conclusions
Diseases commonly treated with antibiotics are associated with expanded gut resistomes, suggesting that historical exposure to antibiotics has exerted considerable selective pressure for ARG acquisition in disease-associated strains.
ASSESSMENT OF GREENHOUSE GAS EMISSIONS IN CRIMEAN FORESTS UNDER THE INFLUENCE OF CROWN AND SURFACE FIRES
Authors: Kobechinskaya Valentina G. , Yarosh Olga B. , Apostolov Valery L. 
Published: July 2023

We present results of studies carried out from 2007 to 2020 on the assessment of the emission of the main greenhouse gases: carbon dioxide, carbon monoxide, methane, nitrous oxide, mono- and nitrogen dioxide, emitted during top and bottom fires in the forests of the Republic of Crimea.
The aim of the work was to monitor greenhouse gas emissions as a result of fires according to ground-based studies in the forests of the peninsula.
Materials and methods. The objects of study were uneven-aged burnt forests of pine plantations and oak forests in the foothill zone (Simferopol forestry), as well as pine and oak forests of the reserve located on the southern macroslope of the Crimean Mountains. The weight indicators of carbon emissions from fires for forest stand, undergrowth, undergrowth and litter are calculated taking into account conversion factors.
Results. The total emissions of greenhouse gases from fires of different intensities in the foothill zone during this period reached 1575.6 t in (9.87 t/ha), and the contribution of carbon dioxide to the total volume was 6.1 t/ha. In the nature reserve, the total emissions were 23227.8 t in (20.98 t/ha) and 12.8 t/ha for carbon dioxide. These higher values are due to greater age, density and stock of the forest stand. The contributions of various greenhouse gases to the total emissions were: 61.2% for carbon dioxide, 28.5% for nitrogen dioxide, 8.5% for carbon monoxide, and less than 1.8% for methane and nitrous oxide in total.
Conclusions. The method used in this study for calculating greenhouse gas emissions released during fire impact on forest ecosystems can be considered as a good toolkit for a detailed assessment of the amount of greenhouse gas emissions generated during fires of various intensities.
DETERMINATION OF IRIDOID, ANTIOXIDANT ACTIVITY AND PHENOLIC COMPOUNDS BY HPLC METHOD IN PLANTS GENTIANA CRUCIATA L. FROM DIFFERENT GROWTH PLACE
Authors: Bimenyindavyi Estella , Timofeeva Olga A. 
Published: July 2023

Background. The ecological and geographical conditions of the habitat may be responsible for the synthesis and accumulation of various bioactive compounds and, therefore, for biological effects, including the antioxidant properties of medicinal plants. Understanding the impact of environmental factors on bioactive compounds in medicinal plants have great importance for choosing a place for growing and harvesting plants with a high content of bioactive compounds, as well as providing the highest quality raw materials in the pharmaceutical industry.
Objective. To study the accumulation of iridoids, phenolic compounds and antioxidant activity in G. cruciata plant samples, depending on the growing conditions.
Results. The regions of the Republic of Tatarstan with the best quality of medicinal raw materials, as well as factors contributing to the accumulation of various groups of biologically active substances in G. cruciate plants, were identified. Humidity and soil type played a decisive role in the accumulation of iridoids; the synthesis of phenolic compounds was more influenced by humidity than the type of soil. However, since the change in antioxidant activity did not correspond to concentration of phenolic compounds, this may be due to the presence of iridoids, which are considered to be the main substances of the plant of the genus Grecian. Their antioxidant activity has been shown in previous studies. In our studies, soil properties seem to have great influence on the production of studied compounds.
Conclusions. Almetevsky and Laishevsky districts can be chosen as the optimal place for growing and collecting medicinal plants G. cruciata L.
COGNITIONS AND EMOTIONS OF PATIENTS DURING POST-COVID REHABILITATION
Authors: Borisov Oleg V. , Hartfelder Denis V. , Zakharova Anna N. , Komarova Evgenia S. , Dulina Galina S. 
Published: July 2023

Background. A lot of scientific articles have been published since the global COVID-19 pandemic was officially announced by WHO. Although different aspects of COVID-19 impact on the mental sphere were studied, some issues regarding the psychological characteristics of those who have had a coronavirus infection are still not fully revealed. It is noted by many authors that even when the main symptoms of the disease are subsided, symptoms of anxiety, depression, cognitive & mood disorders are still persistent even after the treatment.
Purpose: to study cognitive and emotional spheres of patients who are recovering from coronavirus infection and currently undergoing rehabilitation procedures.
Materials and methods. The study involved 100 patients undergoing medical rehabilitation, 55 of them are recovering from traumatological, neurological and cardiovascular disorders (27 male and 28 female patients, aged 57±10) and 45 patients are recovering from pneumonia caused by coronavirus infection (11 male and 34 female patients, aged 58±12). The study was conducted using the Montreal Cognitive Assessment Scale (MoCA), the Hospital Anxiety & Depression Scale (HADS), and the Health-Activity-Mood Scale (SAN). Statistical analysis of raw data had included calculation of descriptive statistics, Pearson’s correlation coefficient, Student’s t-test for independent groups, Fisher’s φ* transformation and significance of correlation coefficients differences with Fisher’s transformation & Student T-distribution, and was processed with Microsoft Office Excel 2016 build-in data analysis package.
Results. Analysis of MoCA results showed that ratio of severe impairments of memory functions and attention span is higher in post-COVID group of patients. This group also had higher rate of anxiety symptoms and sub-clinical depression state. In both groups more than a half of the patients have average level of anxiety and depression, however patients from post-COVID group are more likely to experience more symptoms of anxiety than non-COVID patients. Patients from post-COVID group are significantly more likely to have a higher scores of anxiety compared to patients in the other group and at the same time they are less likely to have average anxiety level. Differences between groups were discovered for “Health” and “Mood” scales of SAN with higher scores among post-COID patients as well as lower scores for “Activity” scale.
Conclusion. Statistically significant differences between the groups were found; common and specific correlations were found for both groups. The results may be useful for healthcare professionals working with post-COVID patients, and can also be used by psychologists who deal with the problems of social and psychological rehabilitation of post-COVID patients.