bluuebunny/crossref_metadata_2025_split huggingface dataset:
GM‐CSF‐producing CCR2+CCR6+ Th17 cells are pathogenic in dextran sodium sulfate‐induced colitis model in mice
Authors: Ariki Shimpei , Ozaka Sotaro , Sachi Nozomi , Chalalai Thanyakorn , Soga Yasuhiro , Fukuda Chiaki , Kagoshima Yomei , Ekronarongchai Supanuch , Mizukami Kazuhiro , Kamiyama Naganori , Murakami Kazunari , Kobayashi Takashi 
Published: January 2023

AbstractAlthough excessive immune responses by Th17 cells, a helper T cell subset, are implicated in the pathogenesis of inflammatory bowel disease (IBD), the mechanism by which its localization in an inflamed colon is regulated remains unclear. Chemokines and their receptors are involved in the pathogenesis of IBD, however, the relative significance of each receptor on Th17 cells remains unknown. We generated C–C motif chemokine receptor 2 (CCR2) knockout (KO) and CCR6 KO mice in the syngeneic background using the CRISPR/Cas9 system and found that the phenotypes of experimental colitis worsened in both mutant mice. Surprisingly, the phenotype of colitis in CCR2/CCR6‐double knockout (CCR2/6 DKO) mice was opposite to that of the single‐deficient mice, with significantly milder experimental colitis (p < .05). The same was true for the symptoms in CCR6 KO mice, but not in wild type mice treated with a CCR2 inhibitor, propagermanium. Colonic CCR2+CCR6+ Th17 cells produced a potentially pathogenic cytokine GM‐CSF whose levels in the gut were significantly reduced in CCR2/6 DKO mice (p < .05). These results suggest that GM‐CSF‐producing CCR2+CCR6+ Th17 cells are pathogenic and are attracted to the inflamed colon by either CCR2 or CCR6 gradient, which subsequently exacerbates experimental colitis in mice.
Onychomatricoma: a clinicopathological, immunohistochemical, and molecular study of 10 cases highlighting recurrent RB1 deletion and the potential diagnostic value of LEF‐1
Authors: Perrin Christophe , Pedeutour Florence , Coutts Michael , Ambrosetti Damien , Dadone‐Montaudié Bérengère 
Published: January 2023

AimsOnychomatricoma (OM), an uncommon benign fibroepithelial neoplasm of the nail unit, is sometimes diagnostically challenging for clinicians and pathologists. OM consistently expresses CD34, but no specific immunohistohemical markers or recurrent genetic alterations have been identified to date. Recent studies have suggested that Wnt signalling is a key molecular characteristic of OM.Methods and resultsTen cases were analysed: four classical OM including two with pleomorphic cells; two superficial acral fibromyxoma‐like variants of OM; three micropapilliferum variants of OM including one with pleomorphic cells; and one proliferating variant of OM. Immunohistochemically, the spindle cells were positive with CD34 (n = 10) and CD99 (n = 1), with focal reactivity for CD10 (n = 5). The epithelial component of the tumours expressed immunopositivity for LEF‐1. Using array comparative genomic hybridization (aCGH), we demonstrated that all OM, including its variants that were tested (n = 8), harboured a few copy number alterations with losses of whole or part of chromosome 13 including the RB1 gene (n = 8) and chromosome 16 (n = 6).ConclusionWe report a recurrent loss of RB1 (13q) as a possible driver molecular event in OM indicating a relationship between OM and other lesions of the spectrum of the so‐called ‘13q/RB1’ family of tumours. We did not identify a role for the Wnt/beta‐catenin signalling pathway, as has been proposed in a recent study. LEF‐1 could be a potential sensitive and specific marker of OM and should be used in the differential diagnosis between OM, superficial acral fibromyxoma, and the CD34‐positive fibrosing family of tumours.
Effect of draw temperature and flame polishing on birefringence of silica glass fiber
Authors: Hausmann Bronson D. , Hawkins Thomas W. , Ballato John , Tomozawa Minoru 
Published: January 2023

AbstractRecently developed methods for high resolution birefringence measurement have been applied to distinguish between the surface and interior birefringence of silica glass fibers as a function of drawing temperature and initial surface condition for two types of silica glass with different water contents. Fibers were drawn in a water‐free argon environment using graphite heating elements. It was found that fibers drawn at lower temperatures resulted in greater, interior birefringence, in agreement with previous reports. Additionally, it was found that in the case of low‐water silica glass, flame polishing via oxygen–hydrogen mixture and drawn into fibers at lower temperature resulted in significant surface compressive stress upon drawing. This compressive stress may be the result of surface stress relaxation in silica glass that occurs in the presence of water during fiber drawing. In silica glass that contains greater internal hydroxyl impurity concentrations, the interior birefringence as well as the surface stress relaxation was significantly reduced under the same fiber drawing conditions. Characterization of such stress responses provides insight into the effects of common processing techniques as well as impresses the significance of preform processing for consistent fiber production.
Physicochemical, thermal and in vitro digestion characteristics of cricket‐ (Acheta domesticus) substituted corn tortillas
Authors: Alvarez‐Barajas Alejandra , Perez‐Carrillo Esther , de la Rosa‐Millan Julian 
Published: January 2023

SummaryThis research aimed to evaluate how the addition of cricket powder (Acheta domesticus) affected corn tortillas' chemical, nutritional and digestibility characteristics. Tortillas with a 5%, 7.5% and 10% A. domesticus powder (AD) substitution to nixtamalised corn flour (NCF) (w/w) were formulated, produced and characterised. The addition of AD caused significant visual differences in samples. At 10% substitution, the lipid content doubled. AD addition impacted carbohydrate composition by increasing the fibre portion and reducing the starch content. The in vitro starch digestibility was affected by the substitution of AD, increasing the resistant starch fraction, which was strongly correlated (R = 0.99) with the substitution percentage of AD. The presence of amylose–lipid complexes (resistant starch type V) was observed in differential scanning calorimetry in all AD‐substituted samples. The addition of AD decreased the predicted glycaemic index by 15% and increased protein digestibility up to 21%. Results provide new insights for the formulation of cricket‐based products and possible low glycaemic index foods.
Multilayer coatings containing red ginseng dietary fibre improve the survivability and stability of probiotic bacteria
Authors: Jeon Hyeon Ji , You Seung‐hwan , Kwon Mijin , Shin Minhye , Kim Sang‐Kyu , Jung Young Hoon 
Published: January 2023

SummaryMaintaining the viability of probiotics is critical for maximising their functionality. In this study, tara gum, basic amino acids, rice protein and red ginseng dietary fibre were sequentially applied to encapsulate various probiotic strains to improve their viability and enhance their adhesion to intestinal cells. Compared with uncoated control strains, multilayer‐coated probiotic strains exhibited higher survival rates under low pH conditions (maximum survival: 81.8% for Lactobacillus casei) and bile acid conditions (>50% survival for Lactobacillus rhamnosus). Multicoating also increased the ability of probiotic strains to adhere to intestinal epithelial cells. For example, encapsulated L. reuteri exhibited 56.9% adhesion, which was approximately four‐fold higher than that of the uncoated strain. Moreover, compared with the uncoated strains, multicoated strains exhibited significantly improved storage stability under various temperatures after freeze‐drying. Therefore, the quadruple‐coating technology used in this study enhances the viability and stability of probiotic strains, which is a key advantage from the industrial perspective.
Potential cost‐effectiveness of e‐health interventions for treating overweight and obesity in Australian adolescents
Authors: Carrello Joseph , Hayes Alison , Baur Louise A. , Lung Thomas 
Published: January 2023

SummaryBackgroundE‐health, defined as the use of information and communication technologies to improve healthcare delivery and health outcomes, has been promoted as a cost‐effective strategy to treat adolescent overweight and obesity. However, evidence supporting this claim is lacking.ObjectivesAssess the potential cost‐effectiveness of a hypothetical e‐health intervention for adolescents with overweight and obesity.MethodsThe costs and effect size (BMI reduction) of the hypothetical intervention were sourced from recent systematic reviews. Using a micro‐simulation model with a lifetime time horizon, we conducted a modelled cost‐utility analysis of the intervention compared to a ‘do‐nothing’ approach. To explore uncertainty, we conducted bootstrapping on individual‐level costs and quality‐adjusted life years (QALYs) and performed multiple one‐way sensitivity analyses.ResultsThe incremental cost‐effectiveness ratio (ICER) for the e‐health intervention was dominant (cheaper and more effective), with a 96% probability of being cost‐effective at a willingness‐to‐pay (WTP) of $50 000/QALY. The ICER remained dominant in all sensitivity analyses except when using the lower bounds of the hypothetical intervention effect size, which reduced the probability of cost‐effectiveness at a WTP of $50 000/QALY to 51%.ConclusionE‐health interventions for treatment of adolescent overweight and obesity demonstrate very good cost‐effectiveness potential and should be considered by healthcare decision makers. However, further research on the efficacy of such interventions is warranted to strengthen the case for investment.
Long‐term clinical and cost‐effectiveness of a therapist‐supported online remote behavioural intervention for tics in children and adolescents: extended 12‐ and 18‐month follow‐up of a single‐blind randomised controlled trial
Authors: Hollis Chris , Hall Charlotte L. , Khan Kareem , Jones Rebecca , Marston Louise , Le Novere Marie , Hunter Rachael , Andrén Per , Bennett Sophie D. , Brown Beverley J. , Chamberlain Liam R. , Davies E. Bethan , Evans Amber , Kouzoupi Natalia , McKenzie Caitlin , Sanderson Charlotte , Heyman Isobel , Kilgariff Joseph , Glazebrook Cristine , Mataix‐Cols David , Serlachius Eva , Murray Elizabeth , Murphy Tara 
Published: January 2023

BackgroundLittle is known about the long‐term effectiveness of behavioural therapy for tics. We aimed to assess the long‐term clinical and cost‐effectiveness of online therapist‐supported exposure and response prevention (ERP) therapy for tics 12 and 18 months after treatment initiation.MethodsORBIT (online remote behavioural intervention for tics) was a two‐arm (1:1 ratio), superiority, single‐blind, multicentre randomised controlled trial comparing online ERP for tics with online psychoeducation. The trial was conducted across two Child and Adolescent Mental Health Services in England. Participants were recruited from these two sites, across other clinics in England, or by self‐referral. This study was a naturalistic follow‐up of participants at 12‐ and 18‐month postrandomisation. Participants were permitted to use alternative treatments recommended by their clinician. The key outcome was the Yale Global Tic Severity Scale Total Tic Severity Score (YGTSS‐TTSS). A full economic evaluation was conducted. Registrations are ISRCTN (ISRCTN70758207); ClinicalTrials.gov (NCT03483493).ResultsTwo hundred and twenty‐four participants were enrolled: 112 to ERP and 112 to psychoeducation. The sample was predominately male (177; 79%) and of white ethnicity (195; 87%). The ERP intervention reduced baseline YGTSS‐TTSS by 2.64 points (95% CI: −4.48 to −0.79) with an effect size of −0.36 (95% CI: −0.61 to −0.11) after 12 months and by 2.01 points (95% CI: −3.86 to −0.15) with an effect size of −0.27 (95% CI ‐0.52 to −0.02) after 18 months, compared with psychoeducation. Very few participants (<10%) started new tic treatment during follow‐up. The cost difference in ERP compared with psychoeducation was £304.94 (−139.41 to 749.29). At 18 months, the cost per QALY gained was £16,708 for ERP compared with psychoeducation.ConclusionsRemotely delivered online ERP is a clinical and cost‐effective intervention with durable benefits extending for up to 18 months. This represents an efficient public mental health approach to increase access to behavioural therapy and improve outcomes for tics.
Feeding behaviour, locomotion rhythms and blood biochemistry of the neotropical red‐tail catfish (Phractocephalus hemioliopterus)
Authors: de Oliveira Guilherme Helder , Perez Ribeiro Paula Adriane , Prado Verônica Guimarães Landa , Bahiense Raphael Nogueira , Gamarano Pedro Gomes , de Oliveira Camila Gomes , de Almeida Freitas Débora , Costa Leandro Santos 
Published: January 2023

AbstractThe study evaluated the feeding behaviour ofPhractocephalus hemioliopterusthrough the animals' ability to adapt to the self‐feeding system, their preferred feeding times and locomotor activity, as well as the blood biochemistry of juveniles fed in a light/dark cycle. The study was carried out through two experiments, the first of which contained two phases. In experiment 1 – phase I, 24 juveniles (35.28 ± 0.62 g) were distributed in eight 48 l tanks. The tanks were equipped with a self‐feeding system and the experiment consisted of evaluating whether the animals were able to adapt to the self‐feeding system, as well as evaluating the preferred feeding times and locomotor activity of these animals. A feeding challenge to the animals was introduced in phase II, based on the results of phase I. The results of the first phase evidenced a nocturnal feeding preference. Thus, the feeding challenge consisted of measuring whether the animal would feed during the day and how long it would take to adapt. When the animals consumed 100% of the amount of feed provided daily, phase II was ended. In experiment 2, 24 juveniles ofP.hemioliopterus(182.00 ± 14.03 g) were distributed in eight 96 l tanks. This experiment consisted of two treatments with four repetitions, one with exclusive feeding during the middle of the light cycle and another with exclusive feeding in the middle of the dark cycle. At the end, blood samples were collected from the animals for blood biochemistry evaluations. In experiment 1 – phase I, the results showed that the fish adapted very well to the self‐feeding system and had a strictly nocturnal feeding behaviour and locomotor rhythm. When they were submitted to the feeding challenge in phase II, the feed intake was stabilized from the 17th day onwards, proportionally to the nocturnal consumption observed in the first phase, thus demonstrating feeding plasticity. In experiment 2, the feeding times influenced the animals' biochemical parameters. Animals fed during the night had higher values of cholesterol and triglycerides than animals fed during the day. It is concluded thatP.hemioliopterushas fast adaptability to a self‐feeding system, with strictly nocturnal feeding and locomotor behaviours. However, it has feeding plasticity, adapting its behaviour according to food availability. Blood biochemical parameters are influenced by the light/dark feeding cycle.
DiceCT for fishes: recommendations for pairing iodine contrast agents with μCT to visualize soft tissues in fishes
Authors: Kolmann Matthew A. , Nagesan Ramon S. , Andrews James V. , Borstein Samuel R. , Figueroa Rodrigo Tinoco , Singer Randal A. , Friedman Matt , López‐Fernández Hernán 
Published: January 2023

AbstractComputed tomography (CT) scanning and other high‐throughput three‐dimensional (3D) visualization tools are transforming the ways we study morphology, ecology and evolutionary biology research beyond generating vast digital repositories of anatomical data. Contrast‐enhanced chemical staining methods, which render soft tissues radio‐opaque when coupled with CT scanning, encompass several approaches that are growing in popularity and versatility. Of these, the various diceCT techniques that use an iodine‐based solution like Lugol's have provided access to an array of morphological data sets spanning extant vertebrate lineages. This contribution outlines straightforward means for applying diceCT techniques to preserved museum specimens of cartilaginous and bony fishes, collectively representing half of vertebrate species diversity. This study contrasts the benefits of using either aqueous or ethylic Lugol's solutions and reports few differences between these methods with respect to the time required to achieve optimal tissue contrast. It also explores differences in minimum stain duration required for different body sizes and shapes and provides recommendations for staining specimens individually or in small batches. As reported by earlier studies, the authors note a decrease in pH during staining with either aqueous or ethylic Lugol's. Nonetheless, they could not replicate the drastic declines in pH reported elsewhere. They provide recommendations for researchers and collections staff on how to incorporate diceCT into existing curatorial practices, while offsetting risk to specimens. Finally, they outline how diceCT with Lugol's can aid ichthyologists of all kinds in visualizing anatomical structures of interest: from brains and gizzards to gas bladders and pharyngeal jaw muscles.
Behavioural responses of fishes to anthropogenic disturbances: Adaptive value and ecological consequences
Authors: Candolin Ulrika , Rahman Tawfiqur 
Published: January 2023

AbstractAquatic ecosystems are changing at an accelerating rate because of human activities. The changes alter the abundance and distribution of fishes, with potential consequences for ecosystem structure and function. Behavioural responses often underlie these changes in population dynamics, such as altered habitat choice or foraging activity. Here, we present a framework for understanding how and why behaviour is affected by human activities and how the behavioural responses in turn influence higher ecological levels. We further review the literature to assess the present state of the field and identify gaps in our knowledge. We begin with discussing the factors that determine how an individual responds to a change in the environment and whether the response is adaptive or not. In particular, we explain the importance of the evolutionary history of the species. We then search the literature to assess our current knowledge of the impact of human disturbances on the behaviour of fishes and the consequences for ecosystems. The search reveals that much attention has been directed to the impact of human activities on the behaviour of fishes, but that worryingly little is known about the consequences of these responses for populations, communities and ecosystems. Yet, behavioural responses can have profound ecological consequences given that behaviour underly many, if not most, species interactions. Thus, more attention should be paid to the mechanisms and pathways through which behavioural responses influence higher ecological levels. Such information is needed if we are to determine the ultimate effects of human activities on biodiversity and the function and stability of aquatic ecosystems.
The effects of ocean acidification on fishes – history and future outlook
Authors: Sundin Josefin 
Published: January 2023

AbstractThe effects of increased levels of carbon dioxide (CO2) on the Earth's temperature have been known since the end of the 19th century. It was long believed that the oceans' buffering capacity would counteract any effects of dissolved CO2 in marine environments, but during recent decades, many studies have reported detrimental effects of ocean acidification on aquatic organisms. The most prominent effects can be found within the field of behavioural ecology, e.g., complete reversal of predator avoidance behaviour in CO2‐exposed coral reef fish. Some of the studies have been very influential, receiving hundreds of citations over recent years. The results have also been conveyed to policymakers and publicized in prominent media outlets for the general public. Those extreme effects of ocean acidification on fish behaviour have, however, spurred controversy, given that more than a century of research suggests that there are few or no negative effects of elevated CO2 on fish physiology. This is due to sophisticated acid–base regulatory mechanisms that should enable their resilience to near‐future increases in CO2. In addition, an extreme “decline effect” has recently been shown in the literature regarding ocean acidification and fish behaviour, and independent research groups have been unable to replicate some of the most profound effects. Here, the author presents a brief historical overview on the effects of elevated CO2 and ocean acidification on fishes. This historical recap is warranted because earlier work, prior to a recent (c. 10 year) explosion in interest, is often overlooked in today's ocean acidification studies, despite its value to the field. Based on the historical data and the current knowledge status, the author suggests future strategies with the aim to improve research rigour and clarify the understanding of the effects of ocean acidification on fishes.
Genome‐scale angiosperm phylogenies based on nuclear, plastome, and mitochondrial datasets
Authors: Hu Hongyin , Sun Pengchuan , Yang Yongzhi , Ma Jianxiang , Liu Jianquan 
Published: January 2023

ABSTRACTAngiosperms dominate the Earth's ecosystems and provide most of the basic necessities for human life. The major angiosperm clades comprise 64 orders, as recognized by the APG IV classification. However, the phylogenetic relationships of angiosperms remain unclear, as phylogenetic trees with different topologies have been reconstructed depending on the sequence datasets utilized, from targeted genes to transcriptomes. Here, we used currently available de novo genome data to reconstruct the phylogenies of 366 angiosperm species from 241 genera belonging to 97 families across 43 of the 64 orders based on orthologous genes from the nuclear, plastid, and mitochondrial genomes of the same species with compatible datasets. The phylogenetic relationships were largely consistent with previously constructed phylogenies based on sequence variations in each genome type. However, there were major inconsistencies in the phylogenetic relationships of the five Mesangiospermae lineages when different genomes were examined. We discuss ways to address these inconsistencies, which could ultimately lead to the reconstruction of a comprehensive angiosperm tree of life. The angiosperm phylogenies presented here provide a basic framework for further updates and comparisons. These phylogenies can also be used as guides to examine the evolutionary trajectories among the three genome types during lineage radiation.