bluuebunny/crossref_metadata_2025_split huggingface dataset:
Effects of dietary glutamine supplementation on growth performance, intestinal digestive ability, antioxidant status and hepatic lipid accumulation in Xenocypris davidi Bleeker.
Authors: Wang Yuehan , Peng Zuxiang , Yan Lin , Gao Xin , Wu Lin , Cui Shuchang , Zhou Mengyuan , Ren Tongjun , Wang Wei , Sun Shuhao , Han Yuzhe , Sun Hang 
Published: May 2023

Abstract
The effects of different concentrations of glutamine on host performance are important in the context of aquaculture. Here, four diets were fed to Xenocypris davidi Bleeker, with 0, 3, 6, and 9 g/kg of glutamine supplementation, for a period of 8 weeks. The results showed that supplementation with 6 and 9 g/kg of glutamine significantly increased the final body weight (FBW), weight gain rate (WGR), and specific growth rate (SGR) of the fish (P < 0.05). Conversely, there was a decreasing trend observed in the condition factor (CF), viscerosomatic index (VSI), hepatosomatic index (HSI), and feed conversion ratio (FCR) with increased glutamine supplementation. Dietary glutamine was found to improve the intestinal structure and function of X. davidi. This was achieved through an increase in the digestive and antioxidant enzyme activities in the intestine, as well as an increase in the muscle layer thickness (MT), villus height (VH), and villus width (VW) of the intestine. These effects were particularly evident in the 6 and 9 g/kg glutamine supplementation groups. This finding was further corroborated by oil red O staining and the relative areas of lipid droplets. A gradual increase in plasma total protein (TP) concentrations with increasing glutamine supplementation was observed, and was considerably higher in the 9 g/kg group than in the control group (P < 0.05). In contrast, plasma total cholesterol (TC) and triglyceride (TG) concentrations were decreased significantly after fish were fed with diets with appropriate glutamine supplementation levels. Notably, we further constructed the correlation analysis to find and verify the important factors that affect the growth performance of host after feeding glutamine. In the present study, we observed that PEP and AMS (belong to digestive enzymes), MT, VH and VW (belong to intestinal morphology), crude protein (belong to proximate composition) showed significant positive correlation with FBW, WGR, SGR (belong to growth performance, P < 0.05). Overall, these findings suggest that dietary glutamine can enhance host performance in X. davidi, particularly when supplemented at 9 g/kg. This may have important implications for the aquaculture industry, as it can improve the health and growth of farmed fish.
Polyhydrido Copper Nanocluster [Cu14H10(PPh3)8(SPhMe2)3]+ : Symmetry-Breaking by Thiolate Ligands to form Racemic Pairs of Chiral Clusters in Solid-State
Authors: Zeng Shuhuan , Ge Xin , Deng Hongwen , Hao Shuwei , Zhang Zhiye , Teo Boon K. , Sun Cunfa 
Published: May 2023

Abstract
A polyhydrido copper nanocluster, [Cu14H10(PPh3)8(SR)3]+ (HSR = 2,4-dimethylbenzenethiol), adopting a distorted fcc structure is reported. One copper atom is “pulled” from the center of the fcc metal framework by the three thiolate ligands. The twisting of the three thiolate ligands about the threefold axis lowers the symmetry from Oh (Cu14) to C3, forming racemic pairs of intrinsic chiral clusters in solid-state.
RANKL inhibition reduces lesional cellularity, Gαs variant expression and enables osteogenic maturation in fibrous dysplasia
Authors: de Castro Luis , Whitlock Jarred , Michel Zachary , Pan Kristen , Taylor Jocelyn , Szymczuk Vivian , Boyce Brendan , Martin Daniel , Chernomordik Leonid , Collins Mike , Boyce Alison 
Published: May 2023

Abstract
Fibrous dysplasia (FD) is a rare, disabling skeletal disease with no established treatments. Growing evidence supports inhibiting the osteoclastogenic factor receptor activator of nuclear Kappa-B ligand (RANKL) as a potential treatment strategy. In this study, we investigated mechanisms underlying RANKL neutralization with the monoclonal antibody denosumab on FD osteoprogenitors, by evaluating human FD tissue pre- and post-treatment, and in murine in vivo and ex vivo pre-clinical models. Histological analysis of human and mouse tissue demonstrated increased osteogenic maturation, reduced cellularity, and reduced expression of the pathogenic Gαs variant in FD lesions after RANKL neutralization. RNA sequencing of human and mouse tissue supported these findings. Interplay between osteoclasts and mutant osteoprogenitors was further assessed in an ex vivo lesion model, indicating that the proliferation of abnormal FD osteoprogenitors was dependent on osteoclastogenesis. Results from this study demonstrate that, beyond its expected anti-osteoclastic effects, denosumab reduces FD lesion activity by decreasing FD cell proliferation and increasing osteogenic maturation, leading to increased lesional bone formation. These findings highlight an unappreciated role for cellular crosstalk between osteoclasts and pre-osteoblast/osteoblasts as a driver of FD pathology, and demonstrate a novel mechanism of denosumab action in the treatment of bone disease.
TRIAL REGISTRATION: ClinicalTrials.gov NCT03571191
Enhanced nuclear Y-box binding protein-1 expression predicts poor prognosis in close association with mammalian target of rapamycin expression in endometrial cancer
Authors: Yoshimitsu Teruyuki , Nishio Shin , Park Jongmyung , Nasu Hiroki , Matsukuma Ken , Tasaki Kazuto , Katsuda Takahiro , Terada Atsumu , Tsuda Naotake , Kawahara Akihiko , Akiba Jun , Kuwano Michihiko , Ushijima Kimio 
Published: May 2023

Abstract
Background
Enhanced expression of oncogenic Y-box binding protein-1 (YB-1) predicts prognostic outcomes in patients with various human malignancies, including ovarian cancer, in close association with aberrant expression of genes involved cell proliferation, survival, and resistance to anti-cancer therapy. We examined whether YB-1 could predict the prognostic outcomes of patients with endometrial cancer and whether enhanced YB-1 expression affects the expression of mammalian target of rapamycin (mTOR), a key effector of the phosphatidylinositol-3 kinase (PI3K)/protein kinase B (AKT)/mTOR pathway, in endometrial cancer.
Methods
We examined expression levels of YB-1 and mTOR in tumor samples of 166 patients with endometrial cancer who underwent surgery at our hospital, including those with endometrioid grade 1–3 tumors, serous carcinoma, and stage I-IV disease. Expression levels of YB-1 and mTOR were assessed by immunohistochemical analysis. The correlation between expression levels of YB-1 or mTOR and prognosis was confirmed by biostatistical analysis.
Results
The positivity rate of nuclear YB-1 expression was 9.4%. YB-1 expression was significantly associated with poor progression-free survival (P = 0.012) and overall survival (P = 0.003). Among 166 tumors, 59 (35.5%) expressed mTOR. Nuclear YB-1 expression also correlated with mTOR expression (P = 0.006). We observed similar results when examining only patients who underwent adjuvant chemotherapy.
Conclusions
Enhanced nuclear YB-1 expression could predict poor outcomes in endometrial cancer, and was significantly closely associated with enhanced mTOR expression. Herein, we discuss whether activation of the PI3K/AKT/mTOR pathway through enhanced mTOR expression relates to enhanced YB-1 expression, thereby affecting the prognostic status of endometrial cancer.
Adults Feeding on Pollen Diet Influence Biology and Life Table Parameters of the Ectoparasitoid, Habrobracon hebetor
Authors: Hadidi Mohammad Saeid , Askarianzadeh Alireza , Abbasipour Habib 
Published: May 2023

Abstract
Habrobracon hebetor Say (Hym.: Braconidae) is a polyphagous and gregarious-ectoparasitoid that can parasitize the larvae of some Lepidoptera pest species. This research studied the effect of feeding adult wasps from different pollen diets on the longevity, fecundity rate, and survival rate of H. hebetor. This experiment was conducted in seven pollen diets with four replications. The diets included control, rapeseed, apple, peach, almond, date, and honeybee pollen. Also, honey and vitamin C were used in all diets. The experiments were performed in controlled conditions. According to the results, pollen diets did not significantly affect longevity. Still, fecundity showed a significant increase in honeybee, rapeseed, and date pollen diets compared to the control. Honeybee and rapeseed pollen diets have the highest increase in fecundity and were in the same group. According to the obtained results of analysis of variance, values of the intrinsic rate of increase (rm), grass reproduction rate (GRR), net reproduction rate (R0), and doubling time (DT) in different pollen diets showed a significant difference. The intrinsic rate of increase of H. hebetor was significantly higher in the almonds pollen diet (0.065 ± 0.003 d− 1) compared to others. The maximum value of grass reproduction rate (GRR) and net reproduction rate (R0) related to the date pollen diet (3.38 ± 0.32 and 2.61 ± 0.39 female/female). The minimum population doubling time (DT) was related to the almonds pollen diet (10.58 ± 0.6 d). Therefore, a diet containing pollen plays an important role in the intrinsic rate of increase and other growth parameters of H. hebetor parasitoids. Totally almond, date palm, rapeseed, and honeybee pollen diets were more suitable for mass rearing the parasitoid wasp.
Blockchain-Cloud Integration: Comprehensive Survey and Open Research Issues
Authors: Ghanmi Houaida , Hajlaoui Nasreddine , Touati Haifa , Hadded Mohamed , Muhlethaler Paul 
Published: May 2023

Abstract

Cloud computing has attracted great interest in various scientific and technical fields recently as one of the widely adopted networking technologies. Despite their many benefits and applications, it still faces many security and trust challenges, including managing and controlling services, privacy, data integrity in distributed databases, data backup, and synchronization. Moreover, due to its centralized architecture, and lack of transparency and traceability, the results of the trust assessment cannot be fully recognized by all users. However, creating a trust-based transaction environment has become its key factor. Blockchain, with its nature of decentralization and security, can be leveraged to address these challenges and build a distributed and decentralized trust architecture, due to the underlying characteristics such as transparency, traceability, decentralization, security, immutability, and automation. This article makes a comprehensive study of how blockchain is applied to deliver security services in the cloud computing model, focusing on up-to-date approaches, opportunities, and future directions. This survey also discusses the benefits of the technical fusion of blockchain and cloud. It provides a classification of proposed systems based on privacy and key sharing, data sharing, authentication and access control, as well as auditing and data integrity. Finally, the main conclusions of this study will be the challenges and future directions to stimulate further research in this promising field.
Mitigation of microbial degradation of X80 carbon steel mechanical properties using a green biocide
Authors: Li Zhong , Yang Jike , Lu Shihang , Dou Wenwen , Gu Tingyue 
Published: May 2023

Abstract
Most microbiologically influenced corrosion (MIC) studies focus on the threat of pinhole leaks caused by MIC pitting. However, microbes can also lead to structural failures. Tetrakis hydroxymethyl phosphonium sulfate (THPS) biocide mitigated the microbial degradation of mechanical properties of X80 pipeline steel by Desulfovibrio ferrophilus, a very corrosive sulfate reducing bacterium. It was found that 100 ppm (w/w) THPS added to the enriched artificial seawater (EASW) culture medium before incubation resulted in approximately 3-log reduction in sessile cell count after a 7-d incubation at 28oC, leading to 94% weight loss reduction. The X80 dogbone coupon incubated with 100 ppm THPS for 7 d suffered only 3% loss in ultimate tensile strain and 0% loss in ultimate tensile strength compared with the abiotic control in EASW. In comparison, the no-treatment X80 dogbone suffered losses of 13% in ultimate tensile strain and 6% in ultimate tensile stress, demonstrating very good THPS efficacy.
Comparisons of Bone and Muscle Performance in Patients with Single or Multiple Vertebral Fractures
Authors: Zhang Chenggui , Li Yang , Wang Guodong , Sun Jianmin 
Published: May 2023

Abstract
Patients with multiple fractures tend to be at higher risk of further fractures and are generally associated with more complex and serious clinical problems than patients with single fractures. Musculoskeletal health of patients with single or multiple vertebral fractures has not been thoroughly compared. Therefore, we aimed to compare the BMD, bone turnover, muscularity, fatty infiltration of muscle, and prevalence of co-morbidities in patients with single and multiple vertebral fractures. We recruited 100 patients with single fracture (age 66.96 ± 8.24 years) and 100 with multiple fractures (age 69.90 ± 7.80 years); performed dual-energy X-ray absorptiometry of the femoral neck, hip, and lumbar vertebrae; and measured biochemical markers of bone turnover, muscularity, and fatty infiltration. Patients with multiple vertebral fractures had lower hip BMD (p = 0.010) than those with single fractures, but there was no difference in femoral neck and lumbar vertebral BMD nor in muscularity. However, fatty infiltration, an indicator of muscle quality, was significantly higher in participants with multiple fractures (p = 0.006). Diabetes was significantly more common in patients with multiple fractures (p = 0.042). There were no significant differences in markers of bone turnover, and Seperman analyses showed no correlations of CTX-1 or tPINP with the BMD of the hip, femoral neck, or lumbar spine. However, high CTX-1 was associated with high tPINP (r = 0.4805; p < 0.0001), and marked fatty infiltration was associated with low hip, lumbar vertebral, and femoral neck BMD. Cox regression analyses showed that age (OR 1.057; 95% CI 1.016–1.101; p = 0.006) and low hip BMD (OR 0.016; 95% CI, 0.000–0.549; p = 0.022) were associated with a higher risk of multiple fractures. In conclusion, hip BMD may be a more sensitive indicator for reflecting the fracture severity, patients with multiple fractures tend to have worse skeletal and muscular health, including lower hip BMD and more fatty infiltration of muscle, implying that they should be targeted clinically.
Old foes following news ways? - Pandemic-related changes in the epidemiology of viral respiratory tract infections
Authors: Maison Nicole , Omony Jimmy , Rinderknecht Sophia , Kolberg Laura , von Both Ulrich , von Mutius Erika , Hübner Johannes , Meyer-Bühn Melanie 
Published: May 2023

Abstract
Introduction
Following lockdown periods and restricting public health measures in response to the COVID-19 pandemic, respiratory tract infections (RTIs) rose significantly worldwide. This led to an increased burden on children's hospitals compromising medical care of acutely and chronically ill children. We characterized changes in the epidemiological pattern of circulating respiratory viral infections.
Methods
We assessed the number of patients with RTIs and the annual distribution of virus detections between 2019 and 2022 based on 4809 clinical samples (4131 patients) from a German pediatric tertiary care-center. We investigated the impact of lockdown periods on spectra of circulating respiratory viruses, pattern of coinfections, age, and seasonality of infections.
Results
A fourfold increase in the number of respiratory virus detections was observed in 2019 vs 2022 with numbers doubling in 2022 (vs 2021). In 2022, seasonal patterns of virus circulation were less pronounced and appeared to have disappeared for Rhino/Enterovirus. SARS-CoV-2, Parainfluenza- and human Metapneumovirus detections increased significantly in 2022 (2019 vs 2022, p < 0.01). Coinfections with multiple viruses occurred more frequently since 2021 compared to pre-pandemic years, especially in younger children (2019 vs 2022, p < 0.01).
Conclusion
Compared to pre-pandemic years, we observed a dramatic increase in pediatric RTIs with an incrementing spectrum of viruses and a predominance in Rhino/Enterovirus infections – leading to a high rate of hospital admissions, particularly in conjunction with other viruses. This caused an acute shortage in medical care and may also be followed by an increase of virus-triggered secondary chronic respiratory diseases like asthma - rendering a burden on the health system.
An Institutional Experience of Haematoma Management After Head and Neck Onco-surgeries
Authors: Dhondge Rajendra , Hussain Mohsina , Shaikh Ahmer Arif , Roy Sirshendu , Nagarkar Raj 
Published: May 2023

Abstract
Management of patients with head and neck squamous cell carcinoma mainly consists of controlling the primary tumour and regional cervical nodal metastases if any. Surgical complications lead to increased hospital stay, further surgical interventions and sometimes a fatal outcome. Postoperative neck hematoma is one such potential and dreaded complication of head and neck surgeries leading to infection, airway obstruction, compression of the surrounding structures causing free flap failure or sometimes may even lead to death. Haematoma might arise from any of the unaddressed neck vessels or some major vessels in the primary site which were inadvertently injured. To minimise the incidence, we in our institute have formulated an institutional protocol and followed it stringently for all patients undergoing neck dissection and free flap reconstruction. Between August 2017 to March 2019 out of 548 patients operated and reconstructed with free flap, 24 patients (4.38%) had developed a post-operative haematoma. However, after following our protocol, the incidence of haematoma drastically decreased and out of 1234 patients operated between April 2019 to December 2022 only 14 patients (1.13%) developed haematoma of the neck. Though ours is a pilot study, we feel that our protocol is easy to follow, and we have applied it successfully in surgical practice and decreased the incidence of post-operative hematoma.
The role of NSE and Tau proteins in identifying Sepsis-associated Delirium: a cross- sectional pilot study
Authors: Pinto Agnes , Carvalho Maira , Barbeiro Hermes , Gomez Luz , Maia Ian , Marchini Júlio Flávio Meirelles , Garcez Flávia Barreto , Avelino-Silva Thiago Junqueira , Soler Lucas , Mochetti Matheus , Souza Heraldo , Alencar Julio Cesar Garcia 
Published: May 2023

AbstractBackground Sepsis-associated delirium is a cerebral manifestation commonly occurring in patients with sepsis and is thought to occur due to a combination of neuroinflammation and disturbances in cerebral perfusion, the blood brain barrier (BBB) and neurotransmission. This cross-sectional pilot study aims to evaluate plasma levels and possible correlation between brain cell damage biomarkers (neuron-specific enolase [NSE] and Tau proteins) in patients with delirium and sepsis, and to obtain tools that identify sepsis in patients with delirium admitted in the Emergency Department. Results We analyzed 25 patients, and 14 (56%) had sepsis. Septic patients had higher NSE (2.7ng/mL, [95% CI: 2.2–3.2] vs. 1.7ng/mL, [95% CI: 0.8–2.5], p < 0.003) and Tau (94.2pg/mL, [95% CI: 77.0-111.3] vs. 57.8pg/mL, [95% CI 31.2–84.5], p < 0.003) than non-septic patients. The best cutoffs for NSE and Tau protein were 2.08ng/mL (LR positive: 4.71, LR negative: 0.17) and 59.27pg/mL (LR positive 3.40, LR negative: 0.09) respectively. We found a 90% specificity for developing sepsis in patients with both NSE above 1.59ng/mL and Tau above 59.27pg/mL. Conclusions NSE and Tau proteins, biomarkers of brain injury, are higher in septic patients than non-septic when analyzed older patients with delirium. Therefore, we suggest that plasma levels of these proteins may be further studied as tools to identify infectious etiology of delirium in older patients in Emergency Departments.
HIPPO: HIstogram-based Pseudo-POtential for scoring protein-ssRNA fragment-based docking poses
Authors: Kravchenko Anna , De Vries Sjoerd Jacob , Smaïl-Tabbone Malika , de Beauchene Isaure Chauvot 
Published: May 2023

Abstract
Motivation
: The RNA-Recognition motif (RRM) is a protein domain that binds single-stranded RNA (ssRNA) and is present in as much as 2% of the human genome. Despite this important role in biology, RRM-ssRNA interactions are very challenging to study on the structural level because of the remarkable flexibility of ssRNA. In the absence of atomic-level experimental data, the only method able to predict the 3D structure of protein-ssRNA complexes with any degree of accuracy is ssRNA’TTRACT, an ssRNA fragment-based docking approach using ATTRACT. However, this approach has limitations, such as the production of only a handful of near-native poses amid many non-natives, and the frequent failure of the ATTRACT scoring function (ASF) to recognize these near-natives. Nevertheless, since ASF parameters are not ssRNA-specific and were determined in 2010, there is substantial opportunity for enhancement.
Results
Here we present HIPPO, a composite RRM-ssRNA scoring potential derived analytically from contact frequencies in near-native versus non-native docking models. Validated on a fragment-based docking benchmark of 57 experimentally solved RRM-ssRNA complexes, HIPPO achieved a 3-fold or higher enrichment for half of the fragments, versus only a quarter with ASF. In particular, HIPPO drastically improved the chance of very high enrichment (12-fold or higher), a scenario where the incremental modelling of entire ssRNA chains from fragments becomes viable. However, for the latter result, more research is needed to make it directly practically applicable. Regardless, our approach already improves upon the state of the art in RRM-ssRNA modelling and is in principle extendable to other types of protein-nucleic acid interactions.
Temperature Inhibition of Plasma-Driven Methane Conversion in DBD Systems
Authors: Akintola Ibukunoluwa , Rivera-Castro Gerardo , Yang Jinyu , Secrist Jeffrey , Hicks Jason C. , Veloso Felipe , Go David B. 
Published: May 2023

Abstract
Low-temperature non-thermal plasmas (LTPs) produce highly reactive chemical environments made up of electrons, ions, radicals, and vibrationally excited molecules. These reactive species, when combined with catalysts, can help drive thermodynamically unfavorable chemical reactions at low temperatures and atmospheric pressure. One potential area of impact is the direct coupling of methane (CH4) with nitrogen (N2) to produce value-added chemicals via a plasma-assisted catalytic process. To effectively create these plasma catalytic systems, a fundamental understanding of the plasma-phase chemistry alone is imperative. While there have been many studies on methane plasmas and how certain operating conditions (i.e., gas composition and power) affect the plasma, there is limited understanding on how changing bulk reaction temperature affects the plasma properties and ensuing plasma chemistry. In this work, we use a dielectric barrier discharge (DBD) to investigate the effects of temperature on the reaction chemistry and the plasma’s electrical properties in various methane-gas mixtures. Results show that increasing temperature leads to a reduction in methane conversion as well as changes to both the gas and dielectric material pre-breakdown, which manifests itself in temperature-dependent electrical properties of the plasma. Experiments at various temperatures and power show a positive correlation between key electrical plasma properties (average charge and lifetime per filament) and the measured methane conversion as a function of temperature.