bluuebunny/crossref_metadata_2025_split huggingface dataset:
MiR-488 facilitates wound healing through CYP1B1-mediated Wnt/β-catenin signaling pathway by targeting MeCP2
Authors: Zuo Chenchen , Fan Pengju , Yang Ying , Hu Chengjun 
Published: June 2023

Abstract
Background: Diabetic wounds are difficult to heal, but its pathogenesis has not been elucidated. MicroRNAs (miRNAs) are considered to act as key roles in wound healing. In this paper, the role of miR-488 in wound healing was investigated.
Methods: The mRNA and protein expressions were assessed using RT-qPCR and western blot. The gene methylation was measured by MSP assay. Cell proliferation, apoptosis and migration were assessed using BrdU, flow cytometer and wound healing assay, respectively. Additionally, the angiogenesis ability of HUVEC cells was analyzed using in vitro angiogenesis assay. Dual-luciferase reporter assay was adopted to analyze the interaction between miR-488 and MeCP2.
Results: Here our results displayed that miR-488 and CYP1B1 expressions were markedly reduced in wound tissues of diabetic with skin defect, while MeCP2 was significantly upregulated. Function assays displayed that miR-488 promoted cell proliferation and migration as well as HUVEC cell angiogenesis through regulation of MeCP2, while inhibited the apoptosis. MiR-488 overexpression could also accelerate wound healing in vivo. MeCP2 functioned as the target of miR-488, and suppressed wound healing in vitro. We subsequently confirmed MeCP2 suppressed CYP1B1 expression via promoting its methylation status. In addition, CYP1B1 knockdown inhibited wound healing. Furthermore, MeCP2 overexpression abolished the promoting effect of miR-488 on wound healing. It was also turned out that Wnt4/β-catenin pathway was the downstream pathway of miR-488/MeCP2/CYP1B1 in regulating wound healing.
Conclusion: MiR-488 is a potential therapeutic target for diabetic wound healing, since miR-488 overexpression promoted wound healing through activating CYP1B1-mediated Wnt4/β-catenin signaling pathway by targeting MeCP2.
Forcing the wheel
Authors: Hodor Jędrzej , Trotter William T. 
Published: June 2023

Abstract

Over the past 10 years, there has been considerable interest in exploring questions connecting dimension for posets with graph theoretic properties of their cover graphs and order diagrams, especially with the concepts of planarity and treewidth. Joret and Micek conjectured that if P is a poset with a planar cover graph, then the dimension of P is bounded in terms of the number of minimal elements of P and the treewidth of the cover graph of P. We settle this conjecture in the affirmative. To this end, we strengthen a recent breakthrough result by Blake, Micek, and Trotter, who proved that for each poset P admitting a planar cover graph and a unique minimal element we have dim(P) <= 2 se(P) + 2, where dim(P) stands for the dimension of P and se(P) stands for the maximum order of a standard example contained in P. We prove that dim(P) <= 2 wheel(P) + 2, where wheel(P) stands for the maximum order of a wheel contained in P.
Non-invasive sampling reveals low mitochondrial genetic diversity for a Critically Endangered island endemic species
Authors: Peters Catherine , Geary Matthew , Hosie Charlotte , Nelson Howard , Rusk Bonnie , Chester Anna Muir 
Published: June 2023

Abstract

As an island endemic with a decreasing population, the Critically Endangered Grenada DoveLeptotila wellsiis threatened by accelerated loss of genetic diversity resulting from ongoing habitat fragmentation. Small, threatened populations are difficult to sample directly but advances in molecular methods mean that non-invasive samples can be used. We performed the first assessment of genetic diversity of populations of Grenada Dove by a) assessing mtDNA genetic diversity in the only two areas of occupancy on Grenada, b) defining the number of haplotypes present at each site and c) evaluating evidence of isolation between sites. We used non-invasively collected samples from two locations: Mt Hartman (n = 18) and Perseverance (n = 12). DNA extraction and PCR were used to amplify 1,751 bps of mtDNA from two mitochondrial markers: NADH dehydrogenase 2 (ND2) and Cytochrome b (Cyt b). Haplotype diversity (h) of 0.4, a nucleotide diversity (π) of 0.4 and two unique haplotypes were identified within theND2sequences; one haplotype was identified within theCyt bsequences. Of the two haplotypes identified; the most common haplotype (haplotype A = 73.9%) was observed at both sites and the other (haplotype B = 26.1%) was unique to Perseverance. Our results show low mitochondrial genetic diversity, a non-expanding population and clear evidence for genetically isolated populations. The Grenada Dove needs urgent conservation action, including habitat protection and potentially augmentation of gene flow by translocation in order to increase genetic resilience and diversity with the ultimate aim of securing the long-term survival of this Critically Endangered species.
Digital Transformation in Tanzania's Healthcare Sector: A Systematic Review of Robust Electronic Health RecordsSystems’ Critical Success Factors
Authors: Mwogosi Augustino 
Published: June 2023

Abstract
This study aimed to identify the critical success factors (CSFs) for implementing effective Electronic Health Record Systems (EHRS) in Tanzania's public healthcare system. Through a systematic literature review, the study analyzed various articles published between 2012 and 2022, focusing on social, technical, and environmental factors specific to developing countries. The modified sociotechnical theory provided the theoretical framework, and the reciprocal translation method synthesized the findings. The study identified barriers to EHRS implementation, including political pressures, societal considerations, and a need for more training and dedication among healthcare professionals. Technical factors were also identified, such as inadequate IT infrastructure and high implementation costs. Environmental factors, including policies, strategies, laws, and regulations, impacted EHRS adoption. Based on the analysis, the study suggests several CSFs for successful EHRS implementation. These include recognizing and rewarding the health informatics profession, developing scalable and interoperable EHRs, promoting EHR acceptability among healthcare professionals, and addressing moral and legal issues surrounding EHRS. The findings of this study contribute valuable insights into the environmental, social, and technical factors that hinder the effective adoption of EHRS in underdeveloped nations. By identifying the CSFs and offering potential solutions to the identified challenges, this study guides public health stakeholders and policymakers to develop strategies and plans to overcome obstacles and facilitate the successful implementation of EHRS in Tanzania's public healthcare system and similar contexts.
4-Octyl itaconate protects chondrocytes against IL-1β-induced oxidative stress and ferroptosis by inhibiting GPX4 methylation in osteoarthritis
Authors: Pan Xuekang , Kong Xiangjia , Yu Qi , Jin Zheyuan , Wang Mige , Lu Huigen , Chen Gang 
Published: June 2023

Abstract

The role of oxidative stress and ferroptosis in the pathogenesis of osteoarthritis (OA) is significant. 4-Octyl Itaconate (OI) has been shown to have protective effects against oxidative stress and inflammatory response, and has been identified as a potential therapeutic agent for OA. However, the specific effects of OI on the regulation of chondrocyte degeneration, oxidative stress, and ferroptosis require further investigation. The objective of our study was to examine the effects of OI on IL-1β-induced chondrocytes and an OA mouse model. Our results demonstrate that OI effectively mitigates IL-1β-induced chondrocyte degeneration in a dose-dependent manner. Additionally, OI exhibited a significant inhibitory effect on ROS production and mitigated the decline in Recombinant Glutathione Peroxidase 4 (GPX4) levels induced by IL-1β and Erastin, a ferroptosis activator. As a result, OI demonstrated potential in attenuating the degenerative effects of IL-1β and Erastin on chondrocytes, possibly through the reduction of ferroptosis. The molecular mechanism underlying OI's regulation of GPX4 expression in chondrocytes was found to involve the repression of GPX4 methylation. Furthermore, the inhibition of GPX4 methylation was observed to improve IL-1β-triggered degeneration, oxidative stress, and ferroptosis in chondrocytes. Comparable outcomes were obtained in animal models of osteoarthritis (OA). The utilization of OI and Ferrostatin-1 (Fer-1) mitigated the progression of OA and cartilage degeneration in the mouse model of OA, which was established by destabilization of medial meniscus (DMM). Consequently, it can be inferred that OI alleviates degeneration, oxidative stress, and ferroptosis in IL-1β-treated chondrocytes by inhibiting GPX4 methylation both in vitro and in vivo. These findings indicate that OI represents a promising new therapeutic modality for OA.
Ultrasound evaluation of diaphragmatic function in patients with idiopathic pulmonary fibrosis: a retrospective observational study
Authors: Milesi Jules , Boussuges Alain , Habert Paul , Bermudez Julien , Reynaud-Gaubert Martine , Delliaux Stéphane , Bregeon Fabienne , Coiffard Benjamin 
Published: June 2023

Abstract
Background
The diaphragm function assessed by ultrasound has been well-studied in COPD, asthma, and intensive care. However, there are only a few studies on diffuse interstitial lung disease, while dyspnea and quality of life are major issues in the management that may depend on the diaphragm.
Methods
We retrospectively included idiopathic pulmonary fibrosis (IPF) patients followed in our center (Marseille, France) between January 2020 and February 2023 who underwent diaphragmatic ultrasound. Our objectives were to describe the diaphragmatic function of IPFs compared to healthy controls and to correlate with clinical, functional, and lung density on CT-scan.
Results
24 IPF patients and 157 controls were included. The diaphragmatic amplitude in IPF was increased at rest (median of 2.20cm vs 1.88cm on the right, p < 0.007, and 2.30cm vs 1.91cm on the left, p < 0.03, in IPF and controls respectively) and decreased in deep breathing (median of 4.85cm vs 5.45cm on the right, p < 0.009, and 5.10cm vs 5.65cm on the left, p < 0.046, in IPF and controls respectively). Diaphragmatic thickness was significantly reduced at rest on the right side (median of 1.75mm vs 2.00mm, p < 0.02, in IPF and controls respectively) and in deep breathing on both sides compared to controls (mean of 3.82mm vs 4.15mm on the right, p < 0.02, and 3.53mm vs 3.94mm, on the left, p < 0.009, in IPF and controls respectively). Diaphragmatic amplitude in deep breathing was moderate to strongly correlated with FVC, DLCO, and 6MWT and negatively correlated with the dyspnea and lung density on CT scan.
Discussion
The diaphragmatic amplitude and thickness were impaired in IPF compared to controls. Diaphragmatic amplitude is the parameter best correlated with clinical, functional, and lung density criteria. Further studies are needed to determine if diaphragmatic amplitude can be a prognostic factor in IPF.
Trial Registration:
CEPRO 2022-033bis, 08/11/2022, retrospectively registered
Strengthening effect of pea dietary fiber on mechanical properties and degradability of polylactic acid
Authors: Zhang Min , Chen XuanHong , Fan Bei , Su Qian , Zhang YiFan , Zhou Yang , Fan Wei , Zhang RuoYu , Zhang Yan , Li Long , Wang FengZhong 
Published: June 2023

Abstract
Pea dietary fiber (PDF) was filled into polylactic acid (PLA) to prepare PLA/PDF composites with different PDF contents (0%, 2.50%, 5.00%, 7.50%, 10.00%) by hot melt extrusion. The thermodynamic properties, microstructure, degradability of PLA/PDF composites were investigated. Results showed that the continuity, smoothness and mechanical properties of the PLA material structure were improved, which was attributed to the defects of the PLA material structure were significantly compensated by the appropriate amount (≤ 5.00%) of PDF. The degradation experiment exhibited that the degradation rate of PLA could be significantly improved by using PDF as filler. The degradation rate of 10.00% PLA/PDF composite at the end of soil, aqueous soil biodegradation and in vitro degradation experiments was 134.09%, 210.00% and 500.00% higher than that of PLA material containing 0% PDF, respectively. In summary, PLA/PDF composite containing 5.00% PDF had the optimum comprehensive properties in terms of smooth surface, good mechanical properties and excellent degradability. That possessed huge market potential in the field of material processing. This study combined materials science with food science, which broadened the new research ideas in the field of polylactic acid modification and PDF application.
Hepatitis B virus reactivation in seronegative occult hepatitis B patient receiving ibrutinib therapy
Authors: Lam Lok-Ka , Chan Thomas Sau Yan , Hwang Yu-Yan , Mak Lung-Yi , Seto Wai-Kay , Kwong Yok-Lam , Yuen Man-Fung 
Published: June 2023

Abstract
Background: Ibrutinib is a Bruton’s tyrosine kinase (BTK) inhibitor approved for the treatment for several mature B-cell malignancies. Reactivation of hepatitis B virus (HBV) is a well-described complication in patients with chronic HBV infection or prior HBV exposure undergoing cytotoxic or immunosuppressive chemotherapy for hematologic malignancies. This phenomenon has been frequently reported with rituximab. However, published data on the risk of HBV reactivation induced by ibrutinib are scarce. Cases of HBV reactivation in hematologic patients receiving ibrutinib therapy have recently been described, but limited only to overt hepatitis B patients or seropositive occult hepatitis B patients.
Case presentation: We report the first case of HBV reactivation during ibrutinib treatment in an asymptomatic 82-year-old woman with seronegative occult hepatitis B patient (i.e., negative for HBsAg, anti-HBc and anti-HBs). Four months after ibrutinib treatment, her liver function test (LFT) was deranged, with seroconversion to HBsAg positivity. Serum hepatitis B virus DNA was quantified to be 1.92 x 108 IU/ml. Antiviral treatment was initiated, and viral load was gradually suppressed with improvement in LFT.
Conclusions: Our case illustrated that in populations with a high incidence of HBV exposure, systematic screening for HBV exposure is essential prior to ibrutinib treatment, followed by serial monitoring of serologic and molecular markers of hepatitis B. There is a need for an international consensus to support the recommendation of antiviral prophylaxis against HBV reactivation in patients using ibrutinib.
A Qualitative Metasynthesis of Stigma in Women Living with HIV in the United States
Authors: Sommer Sadie , Barroso Julie 
Published: June 2023

Abstract
Our goal was to synthesize qualitative studies on HIV-related stigma as experienced by women living with HIV (WLWH) in the U.S. Qualitative metasynthesis techniques as developed by ANON were used to integrate and update findings on stigma in WLWH in the U.S. in 43 reports of qualitative studies conducted between 2004–2023 with 1118 participants. Developed themes explored a collective narrative of women first surviving the intersectionality of multiple sources of stigma, discovering non-linear pathways to transcend their stigma, and finally experiencing resilience through their transcendence of stigma. While this metasynthesis revealed similarities to an earlier metasynthesis in the ubiquity and persistence of stigma, they differ primarily in women’s abilities to find agency in managing and opposing their stigma. This cognitive reframing of their stigma helped women to redefine stigma as ignorance and move towards a more positive assessment of the self. In doing so, they separated themselves from their stigma and the damaging effects of it. Findings from this metasynthesis may serve as a useful tool for the development of stigma reduction interventions specific to the needs and experiences of WLWH in the U.S.
Investigating factors influencing implementation of tuberculosis infection prevention and control in rural Papua New Guinea
Authors: Marme Gigil , Kuzma Jerzy , Zimmerman Peta-Anne , Harris Neil , Rutherford Shannon 
Published: June 2023

Abstract
Background: Tuberculosis (TB) is a highly transmissible infectious disease killing millions of people yearly. Implementing TB infection prevention and control (TB-IPC) is pivotal to preventing TB transmission in health institutions. This study aimed to investigate diverse stakeholders’ perspectives relating to barriers and strategies of TB-IPC in rural hospitals in Papua New Guinea (PNG).
Methods: Multiple qualitative case studies were conducted with 32 key stakeholders with experience in TB services in rural hospitals. Data collection drew on three primary sources to triangulate data: semi-structured interviews, document reviews, and field notes. The data were analysed using hybrid thematic analysis.
Results: Our results reveal that key stakeholders perceive multiple interdependent factors that affect TB-IPC. The key emerging themes include strategic planning for and prioritising TB-IPC, governance, leadership, and accountability at the provincial and district level, community attitudes towards TB control, institutional capacity to deliver TB, healthcare workers’ safety, and long-term partnership and integration of TB program.
Conclusion: The evidence suggests that a multi-perspective approach to TB-IPC programs is needed for effectively implementing TB-IPC guidelines in healthcare institutions. Interventions focusing on addressing health systems strengthening may improve the implementation of TB-IPC guidelines.
Transcutaneous auricular vagal nerve stimulation alleviated the development of peripheral and central symptoms of systemic lupus erythematosus in MRL/lpr mice
Authors: Lv Hongjie , Wang Ping , Yu Xiu , Luo Mengxian , Luo Yijun , Lu Haimei , Wang Keer , Xi Anran , Wen Chengping , Xu Zhenghao 
Published: June 2023

Abstract
Objective.
The objective of this study was to investigate the effects of transcutaneous auricular vagus nerve stimulation (taVNS) on the development of peripheral and central symptoms of systemic lupus erythematosus (SLE) in MRL/lpr mice.
Methods.
MRL/lpr mice were treated with taVNS for ten weeks, and the severity of both the central and peripheric symptoms was assessed.
Results.
taVNS activated tyrosine hydroxylase positive (TH+) neurons in the locus coeruleus (LC), improved cognitive impairment, and alleviated depression-like behaviors. taVNS reduced the number of hippocampal microglia and protected the LC TH+ neurons in MRL/lpr mice. Treatment with taVNS also retarded the development of lymphadenectasis and splenomegaly, decreased the proportion of double-negative T (DNT) cells, as well as alleviated nephritis and renal IgG deposition in MRL/lpr mice. However, when the LC TH+ neurons were selectively lesioned, both peripheral and central therapeutic action of taVNS was eliminated. Finally, the results of liquid chromatography-tandem mass spectrometry (LC-MS) indicated taVNS mainly LC-dependently reduced the concentration of norepinephrine and adrenaline in serum.
Conclusion.
This study provides direct evidence that taVNS can retard the development of peripheral and central symptoms of SLE, which may be related to its modulating the activity of LC TH+ neurons. Our findings suggest that taVNS may be a potential non-invasive treatment approach for SLE, although further research is needed to explore its clinical applications in human patients.
An interpretable machine learning model for predicting cavity water depth and cavity length based on XGBoost–SHAP
Authors: Mo Tiexiang , Li Shanshan , Li Guodong 
Published: June 2023

Abstract
In contrast to the traditional black box machine learning model, the white box model can achieve higher prediction accuracy and accurately evaluate and explain the prediction results. Cavity water depth and cavity length of aeration facilities are predicted in this research based on Extreme Gradient Boosting (XGBoost) and a Bayesian optimization technique. The Shapley Additive Explanation (SHAP) method is then utilized to explain the prediction results. This study demonstrates how SHAP may order all features and feature interaction terms in accordance with the significance of the input features. The XGBoost–SHAP white box model can reasonably explain the prediction results of XGBoost both globally and locally and can achieve prediction accuracy comparable to the black box model. The cavity water depth and cavity length white box model developed in this study have a promising future application in the shape optimization of aeration facilities and the improvement of model experiments.
Clinical Characteristics of Kawasaki Disease in Children with Different Age
Groups: A Literature Review and Retrospective Study
Authors: Tan Weiping , Huang Baoying , Liao Shan , Jiang Wei 
Published: June 2023

Background and Objective:
Kawasaki disease (KD) is an acute self-limiting systemic vascular disease
commonly observed in children less than 5 years of age. The present study comparatively assesses the clinical
characteristics of children diagnosed with KD in different age groups. Furthermore, a comprehensive literature
review on the clinical features and diagnostic guidelines of KD is performed.

Methods:
This was a retrospective study conducted on the data of KD children admitted to the Sun Yat-Sen Memorial
Hospital, Guangzhou, China, from January 2016 to December 2018. The children were divided into 3
age groups, including children < 1 year of age (group A, n = 66), 1-5 years of age (group B, n = 74), and children
> 5 years of age (group C, n = 14). Complete clinical evaluation, hematological, and cardiovascular assessments
were conducted and compared between the three groups.

Results:
The time of diagnosis, hemoglobin, and neutrophil ratio of children in group A were significantly lower
than the other two groups (p < 0.05), while the platelet count was significantly higher (p < 0.05). The proportion
of incomplete KD (iKD) was the greatest in group A (40.9%), while the proportion of children with increased
coronary Z value and aseptic meningitis was greater than that in group B (p < 0.0167). Group A
showed less patients with KD shock syndrome (KDSS) than the other two groups (p < 0.05). Group B showed
the greatest number of patients with arthralgia compared to the other two groups (p < 0.05). Three groups
showed no significant difference to intravenous immunoglobulin (IVIG) therapy (p > 0.05).

Conclusion:
The younger the age of KD onset, the more atypical the conditions are, with a greater risk of affecting
other systems and a higher incidences of coronary artery disease. An early treatment with glucocorticoids
might be helpful in older children and those with a greater high-risk KD warning score to prevent coronary injury.