bluuebunny/crossref_metadata_2025_split huggingface dataset:
Phenotype fingerprinting of bipolar disorder prodrome
Authors: Shao Yijun , Cheng Yan , Gottipati Srikanth , Zeng-Treitler Qing 
Published: May 2023

Abstract
Background
Detecting prodromal symptoms of bipolar disorder (BD) has garnered significant attention in recent research, as early intervention could potentially improve therapeutic efficacy and improve patient outcomes. The heterogeneous nature of the prodromal phase in BD, however, poses considerable challenges for investigators. Our study aimed to identify distinct prodromal phenotypes or "fingerprints" in patients diagnosed with BD and subsequently examine correlations between these fingerprints and relevant clinical outcomes.

Methods
20,000 veterans diagnosed with BD were randomly selected for this study. K-means clustering analysis was performed on temporal graphs of the clinical features of each patient. We applied what we call “temporal blurring” to each patient image in order to allow clustering to focus on the clinical features, and not cluster patients based upon their varying temporal patterns in diagnosis, which lead to the desired types of clusters. We evaluated several outcomes including mortality rate, hospitalization rate, mean number of hospitalizations, mean length of stay, and the occurrence of a psychosis diagnosis within one year following the initial BD diagnosis. To determine the statistical significance of the observed differences for each outcome, we conducted appropriate tests, such as ANOVA or Chi-square.

Results
Our analysis yielded 8 clusters which appear to represent distinct phenotypes with differing clinical attributes. Each of these clusters also has statistically significant differences across all outcomes (p < 0.0001). The clinical features in many of the clusters were consistent with findings in the literature concerning prodromal symptoms in patients with BD. One cluster, notably characterized by patients lacking discernible prodromal symptoms, exhibited the most favorable results across all measured outcomes.

Conclusion
Our study successfully identified distinct prodromal phenotypes in patients diagnosed with BD. We also found that these distinct prodromal phenotypes are associated with different clinical outcomes.
Vanillic acid alleviates methamphetamine-induced mitochondrial toxicity in cardiac mitochondria via antioxidant activity and inhibition of MPT Pore opening: an in-vitro study
Authors: Shabani Mohammad , Jamali Zhaleh , Bayrami Deniz , Salimi Ahmad 
Published: May 2023

AbstractBackgroundMethamphetamine is widely abused in all parts of the world. It has been reported that short-term and long-term methamphetamine exposure could damage the dopaminergic system and induce cardiomyopathy and cardiotoxicity via mitochondrial dysfunction and oxidative stress. Vanillic acid (VA), a phenolic acid compound derived from plants, is known for its antioxidant and mitochondrial protection properties.MethodsIn the current study we used VA for attenuating of Methamphetamine-induced mitochondrial toxicity in cardiac mitochondria. Isolated mitochondria obtained from rat heart were grouped as: control, methamphetamine (250 µM), VA (10, 50 and 100 µM) was cotreated with methamphetamine (250 µM) and VA (100 µM) alone. After 60 min, mitochondrial fraction including: succinate dehydrogenases (SDH) activity, mitochondrial membrane potential (MMP), mitochondrial swelling, mitochondrial glutathione (GSH), reactive oxygen species (ROS) and lipid peroxidation (LPO) were evaluated.ResultsMethamphetamine exposure significantly disrupted mitochondrial function and induced ROS formation, lipid peroxidation, GSH depletion, MMP collapse and mitochondrial swelling, while VA significantly increased SDH activity as indicator of mitochondrial toxicity and dysfunction. VA also significantly decreased ROS formation, lipid peroxidation, mitochondrial swelling, MMP collapse and depletion of GSH in cardiac mitochondria in the presence of methamphetamine.ConclusionThese findings suggested that VA is able to reduce methamphetamine-induced mitochondrial dysfunction and oxidative stress. Our results demonstrate that VA could potentially serve as a promising and accessible cardioprotective agent against methamphetamine-induced cardiotoxicity, via antioxidant and mitochondrial protection properties.
Autonomous robotic surgery for zygomatic implant placement and immediately loaded implant-supported full-arch prosthesis: a preliminary research
Authors: Li Changjian , Wang Menglin , Deng Huanze , Li Shumao , Fang Xinyu , Liang Yijie , Ma Xihua , Zhang Yue , Li Yanfeng 
Published: May 2023

Abstract
Objectives
A patient with extensive atrophy of the alveolar ridge in the posterior portion of the maxilla was selected to complete an experimental and clinical case of the robotic zygomatic implant to investigate the viability of an implant robotic system in clinical use.

Methods
The preoperative digital information was collected, and the implantation position and personalized optimization marks needed for robot surgery were designed in advance in a repair-oriented way. The resin models and marks of the patient’s maxilla and mandible are all printed in 3D. Custom-made special precision drills and handpiece holders for robotic zygomatic implants were used to perform model experiments and compare the accuracy of the robotic zygomatic implant group (implant length = 52.5 mm, n = 10) with the alveolar implant group (implant length = 18 mm, n = 20). Based on the results of extraoral experiments, a clinical case of robotic surgery for zygomatic implant placement and immediate loading of implant-supported full arch prosthesis was carried out.

Results
In the model experiment, the zygomatic implant group reported an entry point error of 0.78 ± 0.34 mm, an exit point error of 0.80 ± 0.25 mm, and an angle error of 1.33 ± 0.41degrees. In comparison, the alveolar implant group (control group) reported an entry point error of 0.81 ± 0.24 mm, an exit point error of 0.86 ± 0.32 mm, and an angle error of 1.71 ± 0.71 degrees. There was no significant difference between the two groups (p > 0.05). In clinical cases, the average entry point error of two zygomatic implants is 0.83 mm, the average exit point error is 1.10 mm and the angle error is 1.46 degrees.

Conclusions
The preoperative planning and surgical procedures developed in this study provide enough accuracy for robotic zygomatic implant surgery, and the overall deviation is small, which is not affected by the lateral wall deviation of maxillary sinus.
Invasive lobular carcinoma of the breast detected with real-time virtual sonography: a case report
Authors: Ito Yukie , Fujii Kimihito , Saito Masayuki , Banno Hirona , Ido Mirai , Goto Manami , Ando Takahito , Mouri Yukako , Kousaka Junko , Imai Tsuneo , Nakano Shogo 
Published: May 2023

Abstract
Background
Invasive lobular carcinoma (ILC) sometimes presents with unique clinical, pathologic, and radiographic features. In this case report, we describe a patient with ILC, whose initial presentation consisted with symptoms secondary to bone-marrow dissemination. In addition, the breast primary was revealed only by magnetic resonance imaging (MRI) followed by real-time virtual sonography (RVS).

Case presentation
A 51-year-old woman presented to our outpatient clinic with dyspnea on exertion. She had severe anemia (hemoglobin, 5.3 g/dL) and thrombocytopenia (platelet count, 31 × 103/mL). Bone-marrow biopsy was performed to evaluate hematopoietic function. The pathologic diagnosis was bone-marrow carcinomatosis due to metastatic breast cancer. Initial mammography followed by ultrasonography (US) failed to detect the primary tumor. On MRI, a non-mass-enhancement lesion was observed. While second-look US also did not detect the lesion, it was clearly visualized with RVS. We were finally able to biopsy the breast lesion. The pathologic diagnosis was ILC positive for both estrogen receptor and progesterone receptor, with 1 + immunohistochemical staining for human epidermal growth factor receptor 2. This case of ILC was characterized by bone-marrow metastasis. Due to decreased cell adhesion, the risk of bone-marrow metastasis is higher in ILC than in invasive ductal carcinoma, the most prevalent type of breast cancer. Biopsy of the primary lesion, which was initially only detected with MRI, was successfully performed with clear visualization during RVS, which is based on the fusion of MRI and US images.

Conclusion
In this case report and literature review, we describe the unique clinical characteristics of ILC and a strategy for identifying primary lesions that are initially only visualized with MRI.
Regenerated silk fibroin based on small aperture scaffolds and marginal sealing hydrogel for osteochondral defect repair
Authors: Luo Yinyue , Xiao Menglin , Almaqrami Bushra sufyan , Kang Hong , Shao Zhengzhong , Chen Xin , Zhang Ying 
Published: May 2023

Abstract

Background
Osteochondral defects pose an enormous challenge without satisfactory repair strategy to date. In particular, the lateral integration of neo-cartilage into the surrounding native cartilage is a difficult and inadequately addressed problem determining tissue repair’s success.

Methods

Regenerated silk fibroin (RSF) based on small aperture scaffolds was prepared with
n
-butanol innovatively. Then, the rabbit knee chondrocytes and bone mesenchymal stem cells (BMSCs) were cultured on RSF scaffolds, and after induction of chondrogenic differentiation, cell-scaffold complexes strengthened by a 14 wt% RSF solution were prepared for in vivo experiments.

Results
A porous scaffold and an RSF sealant exhibiting biocompatibility and excellent adhesive properties are developed and confirmed to promote chondrocyte migration and differentiation. Thus, osteochondral repair and superior horizontal integration are achieved in vivo with this composite.

Conclusions
Overall, the new approach of marginal sealing around the RSF scaffolds exhibits preeminent repair results, confirming the ability of this novel graft to facilitate simultaneous regeneration of cartilage–subchondral bone.
Subaqueous 3D stem cell spheroid levitation culture using anti-gravity bioreactor based on sound wave superposition
Authors: Park Jung Hwan , Lee Ju-Ro , Park Sungkwon , Kim Yu-Jin , Yoon Jeong-Kee , Park Hyun Su , Hyun Jiyu , Joung Yoon Ki , Lee Tae Il , Bhang Suk Ho 
Published: May 2023

Abstract

Background
Recently, various studies have revealed that 3D cell spheroids have several advantages over 2D cells in stem cell culture. However, conventional 3D spheroid culture methods have some disadvantages and limitations such as time required for spheroid formation and complexity of the experimental process. Here, we used acoustic levitation as cell culture platform to overcome the limitation of conventional 3D culture methods.

Methods
In our anti-gravity bioreactor, continuous standing sonic waves created pressure field for 3D culture of human mesenchymal stem cells (hMSCs). hMSCs were trapped and aggerated in pressure field and consequently formed spheroids. The structure, viability, gene and protein expression of spheroids formed in the anti-gravity bioreactor were analyzed by electron microscope, immunostaining, polymerase chain reaction, and western blot. We injected hMSC spheroids fabricated by anti-gravity bioreactor into the mouse hindlimb ischemia model. Limb salvage was quantified to evaluate therapeutic efficacy of hMSC spheroids.

Results
The acoustic levitation in anti-gravity bioreactor made spheroids faster and more compact compared to the conventional hanging drop method, which resulted in the upregulation of angiogenic paracrine factors of hMSCs, such as vascular endothelial growth factor and angiopoietin 2. Injected hMSCs spheroids cultured in the anti-gravity bioreactor exhibited improved therapeutic efficacy, including the degree of limb salvage, capillary formation, and attenuation of fibrosis and inflammation, for mouse hindlimb ischemia model compared to spheroids formed by the conventional hanging drop method.

Conclusion
Our stem cell culture system using acoustic levitation will be proposed as a new platform for the future 3D cell culture system.

Graphical Abstract
Stakeholder views on the design of National Health Service perinatal mental health services: 360-degree survey
Authors: Scott John , Mcdonald Christopher , McRobbie Sarah , Watt Blair , Young Judith , Morris Jane 
Published: May 2023

Aims and methodAt the start of a new community perinatal mental health service in Scotland we sought the opinions and aspirations of professional and lay stakeholders. A student elective project supported the creation of an anonymous 360-degree online survey of a variety of staff and people with lived experience of suffering from or managing perinatal mental health problems. The survey was designed and piloted with trainees and volunteer patients.ResultsA rich variety of opinions was gathered from the 60 responses, which came from a reasonably representative sample. Respondents provided specific answers to key questions and wrote free-text recommendations and concerns to inform service development.Clinical implicationsThere is clear demand for the new expanded service, with strong support for provision of a mother and baby unit in the North of Scotland. The digital survey method could be adapted to generate future surveys to review satisfaction with service development and generate ideas for further change.
Derivation of a Unique, Algorithm-Based Approach to Cancer Patient Navigator Workload Management
Authors: Zhu Xiyitao , Zhang Peng , Kang Hyojung , Marla Lavanya , Robles Granda Marlene Isabel , Ebert-Allen Rebecca A. , Stewart de Ramirez Sarah , Oderwald Tenille , McGee Mackenzie , Handler Jonathan A. 
Published: May 2023

PURPOSE Cancer patient navigators (CPNs) can decrease the time from diagnosis to treatment, but workloads vary widely, which may lead to burnout and less optimal navigation. Current practice for patient distribution among CPNs at our institution approximates random distribution. A literature search did not uncover previous reports of an automated algorithm to distribute patients to CPNs. We sought to develop an automated algorithm to fairly distribute new patients among CPNs specializing in the same cancer type(s) and assess its performance through simulation on a retrospective data set. METHODS Using a 3-year data set, a proxy for CPN work was identified and multiple models were developed to predict the upcoming week's workload for each patient. An XGBoost-based predictor was retained on the basis of its superior performance. A distribution model was developed to fairly distribute new patients among CPNs within a specialty on the basis of predicted work needed. The predicted work included the week's predicted workload from a CPN's existing patients plus that of newly distributed patients to the CPN. Resulting workload unfairness was compared between predictor-informed and random distribution. RESULTS Predictor-informed distribution significantly outperformed random distribution for equalizing weekly workloads across CPNs within a specialty. CONCLUSION This derivation work demonstrates the feasibility of an automated model to distribute new patients more fairly than random assignment (with unfairness assessed using a workload proxy). Improved workload management may help reduce CPN burnout and improve navigation assistance for patients with cancer.
Collagen Protein Ingestion during Recovery from Exercise Does Not Increase Muscle Connective Protein Synthesis Rates
Authors: AUSSIEKER THORBEN , HILKENS LUUK , HOLWERDA ANDREW M. , FUCHS CAS J. , HOUBEN LISANNE H. P. , SENDEN JOAN M. , VAN DIJK JAN-WILLEM , SNIJDERS TIM , VAN LOON LUC J. C. 
Published: May 2023

ABSTRACT

Introduction
Protein ingestion during recovery from exercise has been reported to augment myofibrillar protein synthesis rates, without increasing muscle connective protein synthesis rates. It has been suggested that collagen protein may be effective in stimulating muscle connective protein synthesis. The present study assessed the capacity of both whey and collagen protein ingestion to stimulate postexercise myofibrillar and muscle connective protein synthesis rates.

Methods
In a randomized, double-blind, parallel design, 45 young male (n = 30) and female (n = 15) recreational athletes (age, 25 ± 4 yr; body mass index, 24.1 ± 2.0 kg·m−2) were selected to receive primed continuous intravenous infusions with l-[ring-13C6]-phenylalanine and l-[3,5-2H2]-tyrosine. After a single session of resistance type exercise, subjects were randomly allocated to one of three groups ingesting either 30 g whey protein (WHEY, n = 15), 30 g collagen protein (COLL, n = 15) or a noncaloric placebo (PLA, n = 15). Blood and muscle biopsy samples were collected over a subsequent 5-h recovery period to assess both myofibrillar and muscle connective protein synthesis rates.

Results
Protein ingestion increased circulating plasma amino acid concentrations (P < 0.05). The postprandial rise in plasma leucine and essential amino acid concentrations was greater in WHEY compared with COLL, whereas plasma glycine and proline concentrations increased more in COLL compared with WHEY (P < 0.05). Myofibrillar protein synthesis rates averaged 0.041 ± 0.010, 0.036 ± 0.010, and 0.032 ± 0.007%·h−1 in WHEY, COLL and PLA, respectively, with only WHEY resulting in higher rates when compared with PLA (P < 0.05). Muscle connective protein synthesis rates averaged 0.072 ± 0.019, 0.068 ± 0.017, and 0.058 ± 0.018%·h−1 in WHEY, COLL, and PLA, respectively, with no significant differences between groups (P = 0.09).

Conclusions
Ingestion of whey protein during recovery from exercise increases myofibrillar protein synthesis rates. Neither collagen nor whey protein ingestion further increased muscle connective protein synthesis rates during the early stages of postexercise recovery in both male and female recreational athletes.
Financial Reform and Public Good Provision: Municipal Bankruptcy Law and the Financing of Hospitals
Authors: Rossi Stefano , Yun Hayong 
Published: May 2023

Does financial reform improve public good provision? We examine state-level adoption of municipal bankruptcy law. After reform, municipalities’ borrowing costs decrease and bonds’ issuance increase, particularly for bonds financing hospitals; hospitals’ investments increase, particularly when using such bonds; local firms’ investment and performance increase, particularly in the construction sector. Ex ante, reform occurs earlier in states with weaker unions, stronger bondholders’ interests, and better courts. Similar factors explain congressional voting on municipal bankruptcy law. These results support the hypothesis that financial reform destroys labor union rents and expands investment, highlighting a novel spillover channel from public finance to the real economy. This paper was accepted by Victoria Ivashina, finance. Funding: This work was supported by the Baffi-CAREFIN Research Center at Bocconi. Supplemental Material: The data files and online appendix are available at https://doi.org/10.1287/mnsc.2023.4800 .
AKLIMATISASI MIKROALGA HIJAU DALAM LIMBAH PETERNAKAN UNTUK MENINGKATKAN PENYISIHAN NUTRIEN DAN PRODUKSI LIPIDA
Authors: Irhamni Irmahni 
Published: May 2023

Mikroalga memiliki kemampuan untuk menyerap berbagai bentuk nitrogen, dan posfor. Penelitian mengenai aklimatisasi mikroalga hijau dalam fotobioreaktor volume 2 liter untuk menyisihkan nutrien dalam limbah cair peternakan dengan konsentrasi limbah yang berbeda, yaitu 25, 50 dan 100%, dan siklus pencahayaan 24 jam dan 12 jam (on/off) telah dilakukan. Hasil penelitian menunjukkan bahwa pertumbuhan mikroalga hijau terbaik diperoleh dari mikroalga teraklimatisasi dengan kandungan biomassa tertinggi sebesar 1,65 gr/L berat kering, dikuti dengan 1,4 dan 1,35 gram/liter berat kering pada kultur limbah cair peternakan 25, 50 dan 100%. Pertumbuhan alga hijau tanpa aklimatisasi sangat lambat dengan masa kultivasi yang sama yaitu 16 hari, kandungan biomassa hanya berkisar antara 0,65–1,1 gram/liter berat kering. Proses metabolisme mikroalga hijau teraklimatisasi terbukti mampu menyisihkan nutrien amonium, nitrat dan pospat dalam limbah cair peternakan 60-98%, sehingga dapat disimpulkan bahwa pemanfaatan mikroalga hijau lokal untuk penyisihan limbah peternakan dapat dilakukan sebagai salah satu metode alternatif penanggulangan limbah cair, sedangkan biomassa alga dapat dimanfaatkan untuk produksi lipid minyak alga.
PENURUNAN KANDUNGAN C ORGANIK DAN PEMBENTUKAN GAS PADA PROSES PENGOMPOSAN ECENG GONDOK
Authors: Wilujeng Susi A. 
Published: May 2023

Aktivitas domestik, industry dan pertanian menyebabkan sebagian besar sungai di Indonesia telah terkontaminasi oleh air limbah organik. Hal ini menyebabkan enceng gondok tumbuh subur di sumber-sumber air seperti sungai dan danau. Tujuan penelitian ini adalah menentukan laju penurunan kadar karbon organic untuk menghasilkan biogas yang dapat dimanfaatkan sebagai energy alternarif. Percobaan dilakukan pada tiga reactor, dua reactor uji (Reaktor A dan B) dan satu reaktor kontrol (Reaktor C). Bahan baku eceng gondok sebanyak 15 kilogram per reaktor. Reaktor A ditambahkan Mikroorganisme M-16 dan dilakukan pengadukan. Reaktor B ditambahkan Mikroorganisme M-16 dan tanpa pengadukan. Pengadukan dilakukan sepuluh putaran dengan memutar drum 360 ° setiap hari. Penelitian dilakukan selama 60 hari. Parameter yang dianalisa adalah suhu, kadar air, pH dan produksi gas yang diukur setiap hari. Karbon dan Nitrogen diukur setiap 5 hari sekali. Hasil penelitian menunjukkan penurunan kandungan karbon pada Reaktor A 2,7 lebih cepat dan Reaktor B 2 lebih cepat dibandingkan Reaktor C. Pembentukan biogas pada reactor A 1,8 lebih banyak jika dibandingkan dengan reactor C dan Reaktor B 1,4 lebih banyak jika dibandingkan dari Reaktor C.
KAJIAN SISTEM PENGELOLAAN LIMBAH BAHAN BERBAHAYA DAN BERACUN (B3) KEGIATAN PENDIDIKAN DI KAMPUS INSTITUT TEKNOLOGI SEPULUH NOPEMBER (ITS)
Authors: Wilujeng Susi Agustina 
Published: May 2023

Institut Teknologi Sepuluh Nopember (ITS) memiliki memiliki total 54 laboratorium yang tersebar di empat fakultas dan laboratorium Direktorat Riset dan Pengabdian kepada Masyarakat (DRPM) ITS. Semua laboratium aktif digunakan untuk kegiatan praktikum maupun menunjang penelitian. Kegiatan tersebut tentunya menghasilkan limbah. Limbah yang dihasilkan dari kegiatan di laboratoium dikategorikan sebagai limbah B3 yang dalam pengelolaannya memiliki ketentuan khusu. Saat ini, masing-masing laboratorium sudah memiliki sistem pewadahan untuk limbah-nya, akan tetapi belum semuanya memenuhi standar dan persyaratan sesuai dengan peraturan perundang-undangan yang berlaku. Beberapa departemen di ITS sudah memiliki IPAL khusus untuk limbah laboratorium akan tetapi belum beroperasi dengan baik.
Selain limbah dari kegiatan laboratorium, ITS juga menghasilkan limbah B3 jenis sampah spesifik. Limbah tersebut dihasilkan dari kegiatan perkantoran dan kegiatan kemahasiswaan. Timbulan sampah spesifik di ITS diantaranya adalah berasal dari sampah elektronik dan sampah yang mengandung limbah B3 seperti lampu, catridge printer, baterai, dan bekas kemasan yang mengandung limbah B3.
Penelitian ini adalah penelitian kebijakan yang hasil akhirnya adalah Desain perencanaan TPS limbah B3 disesuaikan dengan kebutuhan dan kapasitas limbah B3 yang dihasilkan pada unit-unit terkait agar dapat menampung limbah B3 dengan jumlah yang sesuai. Perencanaan TPS limbah B3 juga dilengkapi dengan fasilitas-fasilitas tertentu, seperti melakukan standarisasi kemasan/ container yang aman untuk berbagai karakteristik limbah B3, rak penyimpanan kemaan limbah laboratorium, system monitoring, SOP (Standard Operating Procedure), penyediaan label dan simbol seseuai dengan karakteristik limbah, ventilasi yang baik, saluran pengumpul tumpahan, serta sarana pendukung K3 (kotak APD, kotak P3K, emergency eye wash, emergency body wash, APAR).