bluuebunny/crossref_metadata_2025_split huggingface dataset:
Prelicensure Nursing Students’ Attitudes Regarding Breastfeeding Education
Authors: Uhr Stephanie D. , Johnson Teresa S. , Taani Murad H. 
Published: September 2023

Abstract
Breast milk feeding is vital to the health outcomes of the breastfeeding dyad. Nurses have a significant role to promote, educate, and support breastfeeding practices for breastfeeding dyads in diverse settings. Nurses should also promote breastfeeding awareness to normalize breastfeeding as the optimal food for infants. This pilot study investigated the effects of a basic breastfeeding educational module on the breastfeeding attitudes of prelicensure nursing students. There was a statistically significant change in attitudes from pretest and posttest. Developing a comprehensive breastfeeding module is a significant step to standardize education and promote breastfeeding best practices.
Effect of the Endometrial Thickness on the Live Birth Rate: Insights From 959 Single Euploid Frozen Embryo Transfers Without a Cutoff For Thickness
Authors: Ata Baris , Linan Alberto , Kalafat Erkan , Ruíz Francisco , Melado Laura , Bayram Asina , Elkhatib Ibrahim , Lawrenz Barbara , Fatemi Human M. 
Published: September 2023

ABSTRACT
Although numerous studies have examined whether endometrial thickness (ET) independently affects the live birth rate (LBR) after an embryo transfer, contradictory conclusions have resulted in an unclear answer. It has been hypothesized that a thin endometrium decreases implantation rates possibly due to elevated oxygen concentration from spiral arteries; however, this mechanism has not been unequivocally demonstrated.
This retrospective analysis aimed to investigate whether ET independently affects the LBR after a frozen embryo transfer cycle. All patients who underwent a single euploid blastocyst transfer between March 2017 and March 2020 at a single-assisted reproductive technology clinic were included. Live birth was defined as the delivery of a live fetus after 22 weeks of gestation. Programmed cycle (PC) or natural cycle (NC) for endometrial preparation was conducted at physician’s and patient’s discretion. The presence of a linear relationship between ET and LBR was assessed by conditional density plots (CDPs), and receiver operating characteristics (ROC) curve analyses were used to identify whether a threshold of the ET existed to predict the occurrence of live birth. The CDPs were analyzed for an optimal range of the ET that could be associated with a higher LBR, and the distribution of cycle characteristics and embryo quality in that range were compared with cycles outside of that range to identify confounders. Logistic regression models were constructed separately for the PC and NC.
A total of 959 single euploid FETs were included in this analysis, of which 33% (n = 315) were NC and 67% (n = 644) were PC with an overall LBR of 47.1% (452/959). No linear relationship between the ET and LBR or threshold below which the LBR decreased perceivably was identified. In addition, ROC analysis did not suggest a predictive value of the ET for occurrence of live birth based on endometrial preparation or in the overall cohort. Similarly, logistic regression analyses showed no independent effect of the ET on LBR.
The results of this study suggest that there is no linear relationship between the ET and LBR or a clinically significant cutoff for ET below which the LBR decreases in FET cycles. Significantly, the common practice of canceling embryo transfers when the ET is <7 mm is not supported by these data.
Computational drug prediction in hepatoblastoma by integrating pan-cancer transcriptomics with pharmacological response
Authors: Failli Mario , Demir Salih , Del Río-Álvarez Álvaro , Carrillo-Reixach Juan , Royo Laura , Domingo-Sàbat Montserrat , Childs Margaret , Maibach Rudolf , Alaggio Rita , Czauderna Piotr , Morland Bruce , Branchereau Sophie , Cairo Stefano , Kappler Roland , Armengol Carolina , di Bernardo Diego 
Published: September 2023

Background and Aims:
Hepatoblastoma (HB) is the predominant form of pediatric liver cancer, though it remains exceptionally rare. While treatment outcomes for children with HB have improved, patients with advanced tumors face limited therapeutic choices. Additionally, survivors often suffer from long-term adverse effects due to treatment, including ototoxicity, cardiotoxicity, delayed growth, and secondary tumors. Consequently, there is a pressing need to identify new and effective therapeutic strategies for patients with HB. Computational methods to predict drug sensitivity from a tumor's transcriptome have been successfully applied for some common adult malignancies, but specific efforts in pediatric cancers are lacking because of the paucity of data.

Approach and Results:
In this study, we used DrugSense to assess drug efficacy in patients with HB, particularly those with the aggressive C2 subtype associated with poor clinical outcomes. Our method relied on publicly available collections of pan-cancer transcriptional profiles and drug responses across 36 tumor types and 495 compounds. The drugs predicted to be most effective were experimentally validated using patient-derived xenograft models of HB grown in vitro and in vivo. We thus identified 2 cyclin-dependent kinase 9 inhibitors, alvocidib and dinaciclib as potent HB growth inhibitors for the high-risk C2 molecular subtype. We also found that in a cohort of 46 patients with HB, high cyclin-dependent kinase 9 tumor expression was significantly associated with poor prognosis.

Conclusions:
Our work proves the usefulness of computational methods trained on pan-cancer data sets to reposition drugs in rare pediatric cancers such as HB, and to help clinicians in choosing the best treatment options for their patients.
Olfactory and gustatory dysfunction, evaluation and the impact on quality of life among COVID-19 patients: a multi-centre study
Authors: AlEnazi Abdulaziz Saud , Alharbi Maha Abdullah , Althomaly Danah Hamdan , Ashoor Mona Mohammed saleh , Alwazzeh Marwan Jaber , Halawani Roa Talal , Buohliqah Lamia Abdulwahab , Telmesani Laila Mohammed 
Published: September 2023

Background:
Olfactory and gustatory dysfunctions are frequently reported symptoms among COVID-19 patients. However, several reports suggested that there might be significant variation in the prevalence and clinical picture of chemosensory dysfunction in COVID-19 patients among different population.

Objective:
To study the prevalence of chemosensory dysfunction, recovery time and its impact on quality of life (QoL) among COVID-19 patients in Saudi population.

Methods:
This multi-centre observational study was conducted at three COVID-19 centres in Saudi Arabia. Epidemiological and clinical data were extracted at baseline and within the 2-month post-infection. Olfactory and gustatory dysfunctions were assessed via valid taste and smell questionnaire, electronically collected via online survey. Short version of questionnaire of Olfactory disorders-negative statements (sQOD-NS) was used to assess the impact on QoL.

Result:
Total 1734 patients [926 males and 808 females, the mean age of patients was 37.7±11.6 years] with laboratory confirmed COVID-19 were recruited for this study. Chemosensory dysfunction was reported in 56.5% cases. olfactory and gustatory dysfunctions were significantly high in females (66.2%) and age group younger than or equal to 40 years (62.2%). Among patients with olfactory dysfunction and gustatory dysfunction, recovery rate was 757 (77.2%) and 702 (71.6%). Furthermore, the recovery time was within 8 days of onset of symptoms in 53.6% and 61.3% of olfactory dysfunction and gustatory dysfunction cases, respectively. Overall mean QoL score indicated Olfactory and gustatory dysfunction has significant impact on QoL [11.3±6.2 (P value<0.001)]. female as compared to males (12.8±7). Females had significant impact on QoL (11.4±6.6) as compared to males [12.8±7 (P value<0.001)].

Conclusion:
Chemosensory dysfunction among Saudi population was comparable to the European data and significantly higher than Asian supporting the fact that these symptoms vary as per ethnicity. Olfactory and gustatory dysfunction significantly impaired QoL and could present as an early symptom of COVID-19. Recovery rate of these symptoms can serve as a good prognostic data for patient’s counselling. Further long-term follow-up studies would lead to better understanding of prognosis and clinical outcomes.
Prevalence and risk factors of post-cholecystectomy surgical site infections
Authors: Masaod Ruba E. , Salih Mugahid A. 
Published: September 2023

Objectives:
The objectives of this study were to measure the prevalence of post-cholecystectomy surgical site infection and identify the associated risk factors and their association with its prevalence.

Method:
A cross-sectional analytical study including all patients who underwent cholecystectomy in the period from January 2021 to March 2022. The data sheet was filled with records of the patients, and some questions were asked of the patients directly. Many risk factors were assessed and measured in their association with the development of postoperative SSI.

Results:
One hundred seventy-two patients with a mean age of 46.41±13.37 participated in the study. Thirty-five (20.3%) of them were males, and 137 (79.7%) were females. Open cholecystectomy 121 (70.3%) was done more than laparoscopic cholecystectomy 51 (29.7%). The most common indication for cholecystectomy was found to be both cholecystitis and cholelithiasis (53.5%). Out of 172 patients, postoperative wound infection [surgical site infection (SSI)] developed in 29 (16.9%) patients. Of these, 8 (27.6%) were males, while 21 (72.4%) were females, with a mean age of 46.38 (SD=14.12) years. Prophylactic antibiotics intraoperatively and therapeutic antibiotics postoperatively were found to decrease the risk of developing SSI [P=0.005, odds ratio (OR)=0.073] (P=0.012, OR=0.153), respectively. However, hospital stay after surgery (<1 week) was also found to decrease the risk (P=0.001, OR=0.179).

Conclusions:
The prevalence of post-cholecystectomy SSI is high despite a small sample size in comparison with other studies. Prophylactic antibiotics and short hospital stays have an important role in decreasing the risk of developing postoperative SSI.
Uterine Prolapse in Pregnancy: A Review
Authors: Norby Nicole , Murchison Amanda B. , McLeish Shian , Ghahremani Taylor , Whitham Megan , Magann Everett F. 
Published: September 2023

Importance
Although not a common occurrence, uterine prolapse during pregnancy can have significant effects for pregnancy outcomes and quality of life of maternal patients. Most data about management exist as case reports; a review of these cases provides some guidance about treatment options.

Objectives
This review examines current literature about uterine prolapse during pregnancy to assess current information about this condition, prevalence, diagnosis, management, and outcomes.

Evidence Acquisition
Electronic databases (PubMed and Embase) were searched using terms “uterine prolapse” AND “pregnancy” AND “etiology” OR “risk factors” OR “diagnosis” OR “therapy” OR “management” limited to the English language and between the years 1980 and October 31, 2022.

Results
Upon review of 475 articles, 48 relevant articles were included as well as 6 relevant articles found on additional literature review for a total of 54 articles. Of those articles, 62 individual cases of uterine prolapse in pregnancy were reviewed including pregnancy complications, mode of delivery, and outcomes. Prevalence was noted to be rare, but much more common in second and subsequent pregnancies. Most diagnoses were made based on symptomatic prolapse on examination. Management strategies included bed rest, pessary use, and surgery (typically during the early second trimester). Complications included preterm delivery, patient discomfort, urinary retention, and urinary tract infection. Delivery methods included both cesarean and vaginal deliveries.

Conclusions
Although a rare condition, uterine prolapse in pregnancy is readily diagnosed on examination. Reasonable conservative management strategies include observation, attempted reduction of prolapse, and pessary use; if these measures fail, surgical treatment is an option.

Relevance
Our review compiles literature and known cases of uterine prolapse during pregnancy and current evidence about prevalence, diagnosis, management, outcomes, and complications of uterine prolapse during pregnancy in order to inform our target audience in their clinical practice.

Target Audience
Obstetricians and gynecologist, family physicians.

Learning Objectives
After completing this learning activity, the participant should be able to describe the prevalence of uterine prolapse during pregnancy, potential at-risk populations, and presenting symptoms; identify management strategies for uterine prolapse during pregnancy including both surgical and conservative approaches; and assess possible complications of uterine prolapse during pregnancy.
Ectopic Opening of Common Bile Duct to Duodenal Bulb: A Single Tertiary Center Experience
Authors: Ari Derya , Ödemiş Bülent , Başpinar Batuhan , Atay Ali , Erdoğan Çağdaş 
Published: September 2023

Background:
The ectopic opening of the common bile duct(CBD) into the duodenal bulb is a rare biliary anomaly. The study aimed to reveal the experience with clinical and endoscopic outcomes in these patients.

Materials and Methods:
This study was conducted on 57 consecutive patients who underwent endoscopic retrograde cholangiopancreatography (ERCP) for ectopic opening of the CBD into the duodenal bulb at our institution between 2010 and 2020.

Results:
The median age was 59 years (49 males). A total of 146 ERCP procedures were performed (once in 26 patients and 2 or more times in 31 patients). Ten patients had a history of unsuccessful ERCP in an external center. The median follow-up time was 14.6 months. All patients had a slit-like opening of the CBD into the duodenal bulb, apical stenosis, and hook-shaped distal CBD. ERCP findings were CBD stone or dilatation in 55 patients and post-cholecystectomy biliary leakage in 2 patients. Balloon dilatation was performed for apical stenosis in 7 patients and distal CBD stenosis in 26 patients. During the first ERCP session, biliary stent/nasobiliary drainage was placed in 37 patients, and CBD stones were extracted in 19 patients without stenting. Biliodigestive anastomosis was applied to 13 patients, 5 of whom had recurrent cholangitis, 7 required recurrent ERCP, and one was due to the technical difficulty of ERCP.

Conclusions:
Ectopic biliary opening should be remembered if the papilla cannot be seen in its usual place in a patient with apical stenosis. ERCP should be performed in experienced hands, and surgery should be considered in the need for recurrent ERCP.
Pelvic Floor Peritoneum Closure Reduces Severe Postoperative Complications in Rectal Cancer Patients After Laparoscopic Anterior Rectal Resection
Authors: Fu Jie , Zhang Xuehua , Li Gaohua , Xu Zhenzhao , Zhou Jinfan , Yuan Haitao , Xi Jiafei , Wang Yanan 
Published: September 2023

Background:
Laparoscopic anterior rectal resection (LAR) is a commonly performed surgery for rectal cancer patients. Pelvic floor peritoneum closure (PC), a vital procedure in conventional anterior rectal resection, is not routinely performed in LAR.

Study Design:
A total of 1118 consecutive patients with rectal cancer receiving LAR were included in this retrospective study. Patients were allocated into the PC group and the non-PC group. The occurrence of postoperative complications was compared between the 2 groups. Influential factors in anastomotic leakage (AL) were explored using univariate and multivariate logistic regression.

Results:
There was no difference between the groups in terms of baseline characteristics. The occurrence of postoperative complications was similar between the groups. The PC group had significantly shorter postoperative hospitalization and longer operation duration compared with the non-PC group. The occurrences of Clavien-Dindo (CD) III-IV complications, CD III-IV AL, and reoperation were significantly lower in the PC group than the non-PC group. PC and a protective ileostomy were independent protective factors for CD III-IV AL.

Conclusion:
PC could reduce the occurrence of CD III-IV complications, especially CD III-IV AL, and the rate of secondary surgery, especially in patients with a lower body mass index and patients who did not receive protective ileostomies.
The Relation Between Serum-based Systemic Inflammatory Biomarkers and Locoregional Lymph Node Metastasis in Clinical Stages I to II Right-sided Colon Cancers: The Role of Platelet-to-Lymphocyte Ratio
Authors: Lale Azmi , Sahin Ertugrul , Aslan Ahmet , Can Omer Faruk , Ebiloglu Mehmet Fatih , Aygen Erhan 
Published: September 2023

Background:
It aimed to evaluate the relationship between the systemic inflammatory markers and the lymph node metastasis in clinical stages I to II right-sided colon cancers.

Patients and Methods:
A total of 51 eligible clinical stages I to II right-side located colon cancer patients were included in the study. Complete mesocolic excision and central vascular ligation procedures were performed in all cases. All the patients were divided into 2 main groups, pN− (n = 22) and pN+ (n = 29). Demographic parameters, preoperative serum-based inflammatory biomarkers, and histopathological findings were compared between the groups.

Results:
The mean age was 61.0 (54 to 71) years. Of the patients, 51.0% (26/51) were females. The open surgical approach was performed on 54.9% (28/51) of the patients and 45.1% (23/51) was performed laparoscopy. The mean total number of retrieved lymph nodes was 29.1. The lympho-vascular invasion was significantly higher in the pN+ group (89.7% vs 50.0%). There were no significant differences in neutrophil-to-lymphocyte ratio, C-reactive protein-to-albumin ratio, mean platelet volume-to-platelet ratio, hemoglobine-albuminelymphocyte-platelet score, systemic inflammation index, lymphocyte-to-monocyte ratio, neutrophil-to-monocyte ratio, lymphocyte-to-C-reactive protein ratio (LCR), neutrophil-to-albumin ratio, and prognostic nutritional index. However, the mean platelet-to-lymphocyte ratio (PLR) was significantly lower in the pN+ group (pN−: 282.1 vs pN+: 218.7, P = 0.048). The cutoff value for PLR was determined as 220 according to receiver operating characteristic analysis, with a 63.6% sensitivity and 65.6% specificity.

Conclusion:
Although it has limited sensitivity and specificity, decreased preoperative PLR was significantly associated with lymph node metastasis in patients with clinical stages I to II right-sided colon cancer. It should be considered as a biomarker for nodal involvement when planning treatment strategies.
Theory and scientificity of traditional Chinese medicine
Authors: Guo Lanping , Zhang Wenjin , Huang Luqi 
Published: September 2023

Abstract
The Nobel Prize for artemisinin and the COVID-19 pandemic have heightened interest in the disease prevention and treatment of traditional Chinese medicine (TCM). However, uncertainty and misinformation remain about some key issues. Herein, we discuss the current literature showing that, despite TCM gaining recognition, the theoretical systems of TCM and Western medicine rooted in different cultural backgrounds have huge differences in their theories and treatments. How to achieve accurate diagnosis in TCM, how to give scientific treatment, what is the mechanism of treatment, and what are the effect and safety of treatment, all need to be more clearly explained and require high-quality modern scientific evidence. An emerging consensus exists that, with the help of modern technology and scientific methods, it is necessary to excavate the essence of TCM that truly conforms to the laws of human life movement and disease development, realize cross-complementation with Western medicine, enhance human understanding of diseases, enrich diagnosis and treatment methods, and promote a new level in the field of life sciences.
Kaistella polysaccharea sp. nov., isolated from Antarctic intertidal sediment produces a novel extracellular polymeric substance
Authors: Yao Boqing , Niu Guojiang , Wang Zhe , Mu Hongmei , Ren Xingtao , Jiao Yabin , Cai Chao , Li Jing 
Published: September 2023

An exopolysaccharide-producing bacterial strain GW4-15T, belonging to the genus

Kaistella

, was isolated from intertidal sediment from King George Island, Antarctic. The strain was Gram-stain-negative, aerobic, rod-shaped, non-motile and yellow-pigmented. The strain was able to grow in the presence of 0–2 % (w/v) NaCl (optimum, 0 %), at 4–30 °C (optimum, 20–28 °C) and at pH 5.0–10.0 (optimum, pH 8.0). A phylogenetic tree based on 16S rRNA gene sequences showed that strain GW4-15T formed a lineage within the genus

Kaistella

with the closest phylogenetic neighbours

Kaistella carnis

NCTC 13525T (98.3 %),

Kaistella gelatinilytica

G5-32T (97.7 %),

Kaistella antarctica

LMG 24720T (97.4 %) and

Kaistella yonginensis

HMD1043T (96.9 %). Digital DNA–DNA hybridization values of strain GW4-15T with

K. carnis

NCTC 13525T,

K. antarctica

LMG 24720T,

K. gelatinilytica

G5-32T and

K. yonginensis

HMD1043T were 22.8, 22.0, 21.7 and 21.6 %, respectively. The average nucleotide identity values between strain GW4-15T and

K. carnis

NCTC 13525T
, K. antarctica LMG 24720T,

K. gelatinilytica

G5-32T and

K. yonginensis

HMD1043T were 79.3, 78.6, 77.5 and 77.2 %, respectively. The G+C content of the genome was 36.2 mol%. The major phospholipids were phosphatidylethanolamine and aminophospholipid. The predominant menaquinone was MK-6. The major fatty acids were anteiso-C15 : 0 (28.7 %), iso-C16 : 0 3-OH (15.7 %), iso-C16 : 0 H (10.0 %), iso-C16 : 0 (5.4 %), summed feature 9 (comprising iso-C17 : 1
ω9c and/or 10-methyl C16 : 0; 5.2 %) and iso-C15 : 0 (5.1 %). The monosaccharide composition of the new type of extracellular polymeric of GW4-15T was Glc, GalN, GlcN, Rha, Man and Gal with a molar ratio of 3.14 : 3.83 : 8.38 : 5.16 : 1 : 2.82. Based on phenotypic, phylogenetic and genotypic data, a novel species, Kaistella polysaccharea sp. nov., is proposed with the type strain GW4-15T (=CGMCC 1.19368T=KCTC 92753T).
Massively parallelin vivoPerturb-seq reveals cell type-specific transcriptional networks in cortical development
Authors: Zheng Xinhe , Wu Boli , Liu Yuejia , Simmons Sean K. , Kim Kwanho , Clarke Grace S. , Ashiq Abdullah , Park Joshua , Wang Zhilin , Tong Liqi , Wang Qizhao , Xu Xiangmin , Levin Joshua Z. , Jin Xin 
Published: September 2023

AbstractSystematic analysis of gene function across diverse cell typesin vivois hindered by two challenges: obtaining sufficient cells from live tissues and accurately identifying each cell’s perturbation in high-throughput single-cell assays. Leveraging AAV’s versatile cell type tropism and high labeling capacity, we expanded the resolution and scale ofin vivoCRISPR screens: allowing phenotypic analysis at single-cell resolution across a multitude of cell types in the embryonic brain, adult brain, and peripheral nervous system. We undertook extensive tests of 86 AAV serotypes, combined with a transposon system, to substantially amplify labeling and acceleratein vivogene delivery from weeks to days. Using this platform, we performed anin uterogenetic screen as proof-of-principle and identified pleiotropic regulatory networks ofFoxg1in cortical development, including Layer 6 corticothalamic neurons where it tightly controls distinct networks essential for cell fate specification. Notably, our platform can label >6% of cerebral cells, surpassing the current state-of-the-art efficacy at <0.1% (mediated by lentivirus), and achieve analysis of over 30,000 cells in one experiment, thus enabling massively parallelin vivoPerturb-seq. Compatible with various perturbation techniques (CRISPRa/i) and phenotypic measurements (single-cell or spatial multi-omics), our platform presents a flexible, modular approach to interrogate gene function across diverse cell typesin vivo, connecting gene variants to their causal functions.