bluuebunny/crossref_metadata_2025_split huggingface dataset:
Visual augmentation of deck-landing-ability improves helicopter ship landing decisions
Authors: Thomas Mathieu , Serres Julien R. , Rakotomamonjy Thomas , Ruffier Franck , Morice Antoine H. P. 
Published: March 2023

AbstractWhen attempting to land on a ship deck tossed by the sea, helicopter pilots must make sure that the helicopter can develop sufficient lift to be able to safely touchdown. This reminder of affordance theory led us to model and study the affordance of deck-landing-ability, which defines whether it is possible to land safely on a ship deck depending on the helicopter's available lift and the ship’s deck heave movements. Two groups of participants with no piloting experience using a laptop helicopter simulator attempted to land either a low-lifter or a heavy-lifter helicopter on a virtual ship deck by either triggering a pre-programmed lift serving as the descent law if it was deemed possible to land, or aborting the deck-landing maneuver. The deck-landing-ability was manipulated by varying the helicopter's initial altitude and the ship's heave phase between trials. We designed a visual augmentation making visible the deck-landing-ability, and thus enabling participants to maximize the safety of their deck-landing attempts and reduce the number of unsafe deck-landing. The visual augmentation presented here was perceived by participants as a means of facilitating this decision-making process. The benefits were found to have originated from the clear-cut distinction it helped them to make between safe and unsafe deck-landing windows and the display of the optimal time for initiating the landing.
Free androgen index (FAI)’s relations with oxidative stress and insulin resistance in polycystic ovary syndrome
Authors: Rahmatnezhad Leili , Moghaddam-Banaem Lida , Behrouzi Lak Tahereh , Shiva Afshin , Rasuli Javad 
Published: March 2023

AbstractThis study aimed to determine the levels of the free androgen index (FAI) and its association with oxidative stress and insulin resistance (IR) in patients with polycystic ovary syndrome (PCOS). This cross-sectional study was performed on 160 women aged 18–45 years, visiting gynecology clinics of Urmia in northwestern Iran during 2020–2021 who were diagnosed with PCOS and exhibited one of the four phenotypes of PCOS. All the participants underwent clinical examinations, paraclinical tests, and ultrasounds. FAI cut-off point was considered to be 5%. The significance level was set at < 0.05. Among the 160 participants, the prevalence of the four phenotypes was as follows: phenotype A: 51.9%, phenotype B: 23.1%, phenotype C: 13.1%, and phenotype D: 11.9%. High FAI was detected in 30 participants (18.75%). Additionally, It was found that phenotype C had the highest FAI levels among the PCOS phenotypes, with a significant difference between phenotypes A and C (p value = 0.03). IR was observed in 119 (74.4%) of the participants, and the median (interquartile range: IQR) of malondialdehyde (MDA) levels among the participants was 0.64 (0.86) μM/L. In linear regression, the PCOS phenotype (standard beta = 0.198, p-value = 0.008), follicle-stimulating hormone (FSH) levels (standard beta = 0.213, p-value = 0.004), and MDA levels (standard beta = 0.266, p-value < 0.001) were significantly related to the FAI level, but the homeostatic model assessment for insulin resistance (HOMA-IR) was not statistically associated with FAI. Thus, in this study, PCOS phenotypes and MDA levels (an indicator of stress oxidative) were significantly related to FAI, but HOMA-IR (the indicator of IR) was not associated with it.
Superanomalous skin-effect and enhanced absorption of light scattered on conductive media
Authors: Vagov A. , Larkin I. A. , Croitoru M. D. , Axt V. M. 
Published: March 2023

AbstractLight scattering spectroscopy is a powerful tool for studying various media, but interpretation of its results requires a detailed knowledge of how media excitations are coupled to electromagnetic waves. In electrically conducting media, an accurate description of propagating electromagnetic waves is a non-trivial problem because of non-local light-matter interactions. Among other consequences, the non-locality gives rise to the anomalous (ASE) and superanomalous (SASE) skin effects. As is well known, ASE is related to an increase in the electromagnetic field absorption in the radio frequency domain. This work demonstrates that the Landau damping underlying SASE gives rise to another absorption peak at optical frequencies. In contrast to ASE, SASE suppresses only the longitudinal field component, and this difference results in the strong polarization dependence of the absorption. The mechanism behind the suppression is generic and is observed also in plasma. Neither SASE, nor the corresponding light absorption increase can be described using popular simplified models for the non-local dielectric response.
Continuous fish muscle cell line with capacity for myogenic and adipogenic-like phenotypes
Authors: Saad Michael K. , Yuen John S. K. , Joyce Connor M. , Li Xinxin , Lim Taehwan , Wolfson Talia L. , Wu Justin , Laird Jason , Vissapragada Sanjana , Calkins Olivia P. , Ali Adham , Kaplan David L. 
Published: March 2023

AbstractCell-cultivated fish offers the potential for a more ethical, sustainable, and safe seafood system. However, fish cell culture is relatively understudied in comparison to mammalian cells. Here, we established and characterized a continuous Atlantic mackerel (Scomber scombrus) skeletal muscle cell line (“Mack” cells). The cells were isolated from muscle biopsies of fresh-caught fish, with separate isolations performed from two distinct fish. Mack1 cells (cells from the first isolation) were cultured for over a year and subcultured over 130 times. The cells proliferated at initial doubling times of 63.9 h (± 19.1 SD). After a spontaneous immortalization crisis from passages 37–43, the cells proliferated at doubling times of 24.3 h (± 4.91 SD). A muscle phenotype was confirmed through characterization of muscle stemness and differentiation via paired-box protein 7 and myosin heavy chain immunostaining, respectively. An adipocyte-like phenotype was also demonstrated for the cells through lipid accumulation, confirmed via Oil Red O staining and quantification of neutral lipids. New qPCR primers (HPRT, PAX3B, MYOD1, MYOG, TNNT3A, and PPARG) were tailored to the mackerel genome and used to characterize mackerel cell genotypes. This work provides the first spontaneously immortalized fish muscle cell line for research, ideally serving as a reference for subsequent investigation.
Retinal tissue and microvasculature loss in COVID-19 infection
Authors: Kalaw Fritz Gerald P. , Warter Alexandra , Cavichini Melina , Knight Darren , Li Alexandria , Deussen Daniel , Galang Carlo , Heinke Anna , Mendoza Veronica , Borooah Shyamanga , Baxter Sally L. , Bartsch Dirk-Uwe , Cheng Lingyun , Freeman William R. 
Published: March 2023

AbstractThis cross-sectional study aimed to investigate the hypothesis that permanent capillary damage may underlie the long-term COVID-19 sequela by quantifying the retinal vessel integrity. Participants were divided into three subgroups; Normal controls who had not been affected by COVID-19, mild COVID-19 cases who received out-patient care, and severe COVID-19 cases requiring intensive care unit (ICU) admission and respiratory support. Patients with systemic conditions that may affect the retinal vasculature before the diagnosis of COVID-19 infection were excluded. Participants underwent comprehensive ophthalmologic examination and retinal imaging obtained from Spectral-Domain Optical Coherence Tomography (SD-OCT), and vessel density using OCT Angiography. Sixty-one eyes from 31 individuals were studied. Retinal volume was significantly decreased in the outer 3 mm of the macula in the severe COVID-19 group (p = 0.02). Total retinal vessel density was significantly lower in the severe COVID-19 group compared to the normal and mild COVID-19 groups (p = 0.004 and 0.0057, respectively). The intermediate and deep capillary plexuses in the severe COVID-19 group were significantly lower compared to other groups (p < 0.05). Retinal tissue and microvascular loss may be a biomarker of COVID-19 severity. Further monitoring of the retina in COVID-19-recovered patients may help further understand the COVID-19 sequela.
Application of image processing and transfer learning for the detection of rust disease
Authors: Shahoveisi Fereshteh , Taheri Gorji Hamed , Shahabi Seyedmojtaba , Hosseinirad Seyedali , Markell Samuel , Vasefi Fartash 
Published: March 2023

AbstractPlant diseases introduce significant yield and quality losses to the food production industry, worldwide. Early identification of an epidemic could lead to more effective management of the disease and potentially reduce yield loss and limit excessive input costs. Image processing and deep learning techniques have shown promising results in distinguishing healthy and infected plants at early stages. In this paper, the potential of four convolutional neural network models, including Xception, Residual Networks (ResNet)50, EfficientNetB4, and MobileNet, in the detection of rust disease on three commercially important field crops was evaluated. A dataset of 857 positive and 907 negative samples captured in the field and greenhouse environments were used. Training and testing of the algorithms were conducted using 70% and 30% of the data, respectively where the performance of different optimizers and learning rates were tested. Results indicated that EfficientNetB4 model was the most accurate model (average accuracy = 94.29%) in the disease detection followed by ResNet50 (average accuracy = 93.52%). Adaptive moment estimation (Adam) optimizer and learning rate of 0.001 outperformed all other corresponding hyperparameters. The findings from this study provide insights into the development of tools and gadgets useful in the automated detection of rust disease required for precision spraying.
Increasing peak intensity of tropical cyclones passing through the Korean Peninsula
Authors: Basconcillo Joseph , Moon Il-Ju 
Published: March 2023

AbstractAn increasing trend along with an abrupt increase in the peak intensity of tropical cyclones (TCs) passing through the Korean Peninsula (KP) are significantly detected from 1981 to 2020 and since 2003, respectively. Here we present observational evidence that such trend and shift are largely attributed with the increased passages of intense TCs in the KP during the mature boreal autumn (i.e., September–October, SO) and linked with the recent shift of the Pacific Decadal Oscillation (PDO) to its negative phase. A negative PDO during SO is related to environmental changes that are favorable for more intense TC passages in the KP including a weakened East Asian subtropical jet stream, weaker vertical wind shear, warmer subtropical sea surface temperature, and stronger low-level relative vorticity. Such findings are expected to provide new insights on understanding regional TC variability and ultimately, contribute to long range TC prediction initiatives in the KP region.
Magnetic resonance brain volumetry biomarkers of CLN2 Batten disease identified with miniswine model
Authors: Knoernschild Kevin , Johnson Hans J. , Schroeder Kimberly E. , Swier Vicki J. , White Katherine A. , Sato Takashi S. , Rogers Christopher S. , Weimer Jill M. , Sieren Jessica C. 
Published: March 2023

AbstractLate-infantile neuronal ceroid lipofuscinosis type 2 (CLN2) disease (Batten disease) is a rare pediatric disease, with symptom development leading to clinical diagnosis. Early diagnosis and effective tracking of disease progression are required for treatment. We hypothesize that brain volumetry is valuable in identifying CLN2 disease at an early stage and tracking disease progression in a genetically modified miniswine model. CLN2R208X/R208X miniswine and wild type controls were evaluated at 12- and 17-months of age, correlating to early and late stages of disease progression. Magnetic resonance imaging (MRI) T1- and T2-weighted data were acquired. Total intercranial, gray matter, cerebrospinal fluid, white matter, caudate, putamen, and ventricle volumes were calculated and expressed as proportions of the intracranial volume. The brain regions were compared between timepoints and cohorts using Gardner-Altman plots, mean differences, and confidence intervals. At an early stage of disease, the total intracranial volume (− 9.06 cm3), gray matter (− 4.37% 95 CI − 7.41; − 1.83), caudate (− 0.16%, 95 CI − 0.24; − 0.08) and putamen (− 0.11% 95 CI − 0.23; − 0.02) were all notably smaller in CLN2R208X/R208X miniswines versus WT, while cerebrospinal fluid was larger (+ 3.42%, 95 CI 2.54; 6.18). As the disease progressed to a later stage, the difference between the gray matter (− 8.27%, 95 CI − 10.1; − 5.56) and cerebrospinal fluid (+ 6.88%, 95 CI 4.31; 8.51) continued to become more pronounced, while others remained stable. MRI brain volumetry in this miniswine model of CLN2 disease is sensitive to early disease detection and longitudinal change monitoring, providing a valuable tool for pre-clinical treatment development and evaluation.
Charlson comorbidity index applied to shunted idiopathic normal pressure hydrocephalus
Authors: Klinge Petra M. , Ma Kevin L. , Leary Owen P. , Sastry Rahul A. , Sayied Shanzeh , Venegas Ollin , Brinker Thomas , Gokaslan Ziya L. 
Published: March 2023

AbstractA series of epidemiological studies have shown the limited life expectancy of patients suffering from idiopathic normal pressure hydrocephalus (iNPH). In most cases, comorbid medical conditions are the cause of death, rather than iNPH. Though it has also been shown that shunting improves both life quality and lifetime. We sought to investigate the utility of the Charlson comorbidity index (CCI) for improved preoperative risk–benefit assessment of shunt surgery in individual iNPH cases. 208 shunted iNPH cases were prospectively investigated. Two in-person follow up visits at 3 and 12 months assessed postoperative clinical status. The correlation of the age adjusted CCI with survival was investigated over the median observation time of 2.37 years (IQR 1.16–4.15). Kaplan Meier statistics revealed that patients with a CCI score of 0–5 have a 5-year survival rate of 87%, compared to only 55% in patients with CCI > 5. Cox multivariate statistics revealed that the CCI was an independent predictor of survival, while common preoperative iNPH scores (modified Rankin Scale (mRS), gait score, and continence score) are not. As expected, mRS, gait, and continence scores improved during the postoperative follow up period, though relative improvement on any of these was not predicted by baseline CCI. The CCI is an easily applicable preoperative predictor of survival time in shunted iNPH patients. The lack of a correlation between the CCI and functional outcome means that even patients with multiple comorbidities and limited remaining lifetime may appreciate benefit from shunt surgery.
Effects of acidifiers on soil greenhouse gas emissions in calcareous soils in a semi-arid area
Authors: Derafshi Mehdi , Asgari Lajayer Behnam , Hassani Akbar , Dell Bernard 
Published: March 2023

AbstractIn most agricultural fields, when soil pH is high, elemental sulfur or sulfuric acid are used to reduce soil pH and increase the availability of macro and micronutrients for optimum crop yield. However, how these inputs impact soil greenhouse gas emissions is unknown. This study aimed to measure the amount of greenhouse gas emissions and pH after the application of various doses of elemental sulfur (ES) and sulfuric acid (SA). Using static chambers, this study quantifies soil greenhouse gas emissions (CO2, N2O, and CH4) for 12 months after the application of ES (200, 400, 600, 800, and 1000 kg ha−1) and SA (20, 40, 60, 80 and 100 kg ha−1) to a calcareous soil (pH 8.1) in Zanjan, Iran. Also, in order to simulate rainfed and dryland farming which are common practices in this area, this study was conducted with and without sprinkler irrigation. Application of ES slowly decreased soil pH (more than half a unit) over the year whereas application of SA temporarily reduced the pH (less than a half unit) for a few weeks. CO2and N2O emissions and CH4uptake were maximum during summer and lowest in winter. Cumulative CO2fluxes ranged from 1859.2 kg−1CO2-C ha−1 year−1in the control treatment to 2269.6 kg CO2-C ha−1 year−1in the 1000 kg ha−1ES treatment. Cumulative fluxes for N2O-N were 2.5 and 3.7 kg N2O-N ha−1 year−1and cumulative CH4uptakes were 0.2 and 2.3 kg CH4-C ha−1 year−1in the same treatments. Irrigation significantly increased CO2and N2O emissions and, depending on the amount of ES applied, decreased or increased CH4uptake. SA application had a negligible effect on GHGs emissions in this experiment and only the highest amount of SA altered GHGs emissions.
Designing and fabrication of electrochemical nano-biosensor for the fast detection of SARS-CoV-2-RNA
Authors: Hussein Heba A. , Hanora Amro , Solyman Samar M. , Hassan Rabeay Y. A. 
Published: March 2023

AbstractSARS-CoV-2 caused a global panic among populations. Rapid diagnostic procedures for the virus are crucial for disease control. Thus, the designed signature probe from a highly conserved region of the virus was chemically immobilized onto the nanostructured-AuNPs/WO3-screen printed electrodes. Different concentrations of the matched oligonucleotides were spiked to test the specificity of the hybridization affinity whereas the electrochemical impedance spectroscopy was used for tracking the electrochemical performance. After a full assay optimization, limits of detection and quantification were calculated based on linear regression and were valued at 298 and 994 fM, respectively. Further, the high performance of the fabricated RNA-sensor chips was confirmed after testing the interference status in the presence of the mismatched oligos in one nucleotide and completely one. Worthy to mention that the single-stranded matched oligos can be hybridized to the immobilized probe in 5 min at room temperature. The designed disposable sensor chips are capable of detecting the virus genome directly. Therefore, the chips are a rapid tool for SARS-CoV-2 detection.
Co-drawing of technical and high-performance thermoplastics with glasses via the molten core method
Authors: Strutynski Clément , Voivenel Raphaël , Evrard Marianne , Désévédavy Frédéric , Gadret Gregory , Jules Jean-Charles , Brachais Claire-Hélène , Smektala Frédéric 
Published: March 2023

AbstractAmong the different fundamental aspects that govern the design and development of elongated multimaterial structures via the preform-to-fiber technique, material association methodologies hold a crucial role. They greatly impact the number, complexity and possible combinations of functions that can be integrated within single fibers, thus defining their applicability. In this work, a co-drawing strategy to produce monofilament microfibers from unique glass-polymer associations is investigated. In particular, the molten core-method (MCM) is applied to several amorphous and semi-crystalline thermoplastics for their integration within larger glass architectures. General conditions in which the MCM can be employed are established. It is demonstrated that the classical glass transition temperature compatibility requirements for glass-polymer associations can be overcome, and that other glass compositions than chalcogenides can be thermally stretched with thermoplastics, here oxide glasses are considered. Composite fibers with various geometries and compositional profiles are then presented to illustrate the versatility of the proposed methodology. Finally, investigations are focused on fibers produced from the association of poly ether ether ketone (PEEK) with tellurite and phosphate glasses. It is demonstrated that upon appropriate elongation conditions, the crystallization kinetics of PEEK can be controlled during the thermal stretching and crystallinities of the polymer as low as 9 mass. % are reached in the final fiber. It is believed such novel material associations as well as the ability to tailor material properties within fibers could inspire the development of a new class of hybrid elongated objects with unprecedented functionalities.
Oncogenic epidermal growth factor receptor signal-induced histone deacetylation suppresses chemokine gene expression in human lung adenocarcinoma
Authors: Sumimoto Hidetoshi , Takano Atsushi , Igarashi Tomoyuki , Hanaoka Jun , Teramoto Koji , Daigo Yataro 
Published: March 2023

AbstractEpidermal growth factor receptor (EGFR)-mutated (mt) lung adenocarcinoma (LA) is refractory to immune checkpoint inhibitors (ICIs). However, the mechanisms have not been fully elucidated. CD8+ T cell infiltration was significantly lower in EGFR-mt than in EGFR-wild-type LA, which was associated with suppression of chemokine expression. Since this T cell-deserted tumor microenvironment may lead to the refractoriness of ICIs against EGFR-mt LA, we investigated the mechanism by focusing on the regulation of chemokine expression. The expression of C-X-C motif ligand (CXCL) 9, 10 and 11, which constitute a gene cluster on chromosome 4, was suppressed under EGFR signaling. The assay for transposase-accessible chromatin with high-throughput sequencing (ATAC-seq) revealed open chromatin peaks near this gene cluster following EGFR-tyrosine kinase inhibitor (TKI) treatment. The histone deacetylase (HDAC) inhibitor recovered the expression of CXCL9, 10 and 11 in EGFR-mt LA. Nuclear HDAC activity, as well as histone H3 deacetylation, were dependent on oncogenic EGFR signaling. Furthermore, the Cleavage Under Targets and Tagmentation (CUT & Tag) assay revealed a histone H3K27 acetylation peak at 15 kb upstream of CXCL11 after treatment with EGFR-TKI, which corresponded to one of the open chromatin peaks detected by ATAC-seq. The data suggest that EGFR-HDAC axis mediates silencing of the chemokine gene cluster through chromatin conformational change, which might be relevant to the ICI resistance by creating T cell-deserted tumor microenvironment. Targeting this axis may develop a new therapeutic strategy to overcome the ICI resistance of EGFR-mt LA.
Effect of sizes of vinyl modified narrow-dispersed silica cross-linker on the mechanical properties of acrylamide based hydrogel
Authors: Karim Md. Rezaul , Harun-Ur-Rashid Mohammad , Imran Abu Bin 
Published: March 2023

AbstractPolymeric hydrogel with the incorporation of nano to submicro-meter sized materials forms an exhilarating new generation of composite hydrogels. Most of the applications of hydrogels are in aqueous environments in which they swell to a very high degree. This emanates from low density of the polymer chains, making them highly inferior in terms of physical strength and their prospective applications. In order to address the weak mechanical properties, hydrogels have successfully prepared with high tensile strength and toughness by reinforcing the acrylamide (AAm) network with 3-methacryloxypropyltrimethoxysilane (MPTS) modified silica particles (MSiO2) as chemical cross-linker. The MSiO2 cross-linkers are prepared from narrow-dispersed silica particles (SiO2) of 100 nm, 200 nm, and 300 nm diameters to investigate the effect of cross-linker sizes on the mechanical strengths of hydrogels. The presence of MSiO2 remarkably increases the stretching ability and toughness of hydrogels compared to conventional hydrogels. The tensile strength, toughness, and Young’s modulus of the hydrogel decrease from 30 to 11 kPa, 409 to 231 kJ/m3, and 0.16 to 0.11 kPa, respectively, while the SiO2 particle size increase from 100 to 300 nm and the concentration of AAm and MSiO2 (%) are kept constant. The compressive strength and toughness of the hydrogel decrease from 34 to 18 kPa and 6 to 4 kJ/m3, respectively, but the Young’s modulus increases from 0.11 to 0.19 kPa. This work is excellent proof of regulating mechanical strength of hydrogel by adjusting the particle size of MSiO2 cross-linkers.