bluuebunny/crossref_metadata_2025_split huggingface dataset:
Genetic determinants of host tropism inKlebsiellaphages
Authors: Beamud Beatriz , García-González Neris , Gómez-Ortega Mar , González-Candelas Fernando , Domingo-Calap Pilar , Sanjuan Rafael 
Published: March 2023

ABSTRACTBacteriophages play key roles in bacterial ecology and evolution and are potential antimicrobials. However, the determinants of phage-host specificity remain elusive. Here, we used 46 newly-isolated phages to challenge 138 representative clinical isolates ofKlebsiella pneumoniae, a widespread opportunistic pathogen. Spot tests revealed a narrow host range for most phages, with <2% of 6319 phage-host combinations tested yielding detectable interactions. Bacterial capsule diversity was the main factor restricting phage host range. Consequently, phage-encoded depolymerases were key determinants of host tropism, and we identified depolymerase sequence types associated with the ability to infect specific capsular types across phage families. Phages showing a capsule-independent mode of entry exhibited a much broader host range, but their infectivity was still restricted by complex intracellular defense mechanisms. These findings expand our knowledge of the complex interactions between bacteria and their viruses, and have implications for the biomedical and biotechnological use of phages.
Multimodal classification of molecular subtypes in pediatric acute lymphoblastic leukemia
Authors: Krali Olga , Marincevic-Zuniga Yanara , Arvidsson Gustav , Enblad Anna Pia , Lundmark Anders , Sayyab Shumaila , Zachariadis Vasilios , Heinäniemi Merja , Suhonen Janne , Oksa Laura , Vepsäläinen Kaisa , Öfverholm Ingegerd , Barbany Gisela , Nordgren Ann , Lilljebjörn Henrik , Fioretos Thoas , Madsen Hans O. , Marquart Hanne Vibeke , Flaegstad Trond , Forestier Erik , Jónsson Ólafur G , Kanerva Jukka , Lohi Olli , Norén-Nyström Ulrika , Schmiegelow Kjeld , Harila Arja , Heyman Mats , Lönnerholm Gudmar , Syvänen Ann-Christine , Nordlund Jessica 
Published: March 2023

AbstractGenomic analyses have redefined the molecular subgrouping of pediatric acute lymphoblastic leukemia (ALL). Molecular subgroups guide risk-stratification and targeted therapies, but outcomes of recently identified subtypes are often unclear, owing to limited cases with comprehensive profiling and cross-protocol studies. We developed a machine learning tool (ALLIUM) for the molecular subclassification of ALL in retrospective cohorts as well as for up-front diagnostics. ALLIUM uses DNA methylation and gene expression data from 1131 Nordic ALL patients to predict 17 ALL subtypes with high accuracy. ALLIUM was used to revise and verify the molecular subtype of 280 cases with undefined/B-other molecular phenotype, resulting in a single revised subtype for 85.4% of these cases. Our study shows the power of combining DNA methylation and gene expression data for resolving ALL subtypes and provides the first comprehensive population-based retrospective cohort study of molecular subtype frequencies in the Nordic countries, identifying subgroups with differential survival outcomes.
Clinical validation of pneumatic transportation systems for monoclonal antibodies
Authors: Coliat Pierre , Erb Stéphane , Diemer Hélène , Karouby Dan , Banerjee Mainak , Zhu Chen , Demarchi Martin , Cianférani Sarah , Detappe Alexandre , Pivot Xavier 
Published: March 2023

AbstractPneumatic transportation systems (PTS) were recently proposed as a method to carry ready-for-injection diluted monoclonal antibodies (mAbs) from the pharmacy to the bedside of patients. This method reduces transportation time and improves the efficiency of drug distribution process. However, mAbs are highly sensitive molecules for which subtle alterations may lead to deleterious clinical effects. These alterations can be caused by various external factors such as temperature, pH, pressure, and mechanical forces that may occur during transportation. Hence, it is essential to ensure that the mAbs transported by PTS remain stable and active throughout the transportation process. This study aims to determine the safety profile of PTS to transport 11 routinely used mAbs in a clinical setting through assessment of critical quality attributes (CQA) and orthogonal analysis. Hence, we performed aggregation/degradation profiling, post-translational modifications identification using complementary mass spectrometry-based methods, along with visible and subvisible particle formation determination by light absorbance and dynamic light scattering measurements. Altogether, these results highlight that PTS can be safely used for this purpose when air is removed from the bags during preparation.
Underreported in-water behaviours of the loggerhead sea turtle:Foraging on sea cucumbers
Authors: Papafitsoros Kostas 
Published: March 2023

AbstractIn this article, we report on four observations of loggerhead sea turtles (Caretta caretta) foraging on sea cucumbers (Holothuria spp.), on Zakynthos Island, Greece. Direct observations of this behaviour are underreported in the literature. Foraging on such prey was in general a challenging process as the turtles used their flippers and their beak to cut the sea cucumbers albeit without success. They ended up shallowing the sea cucumbers most likely in one intact piece. Even though this behaviour is rare in this site, two out of four observations involved the same male sea turtle, indicating potentially specific dietary preferences for this individual.
PSIICOS projection optimality for EEG and MEG based functional coupling detection
Authors: Altukhov Dmitrii , Kleeva Daria , Ossadtchi Alexei 
Published: March 2023

AbstractFunctional connectivity is crucial for cognitive processes in the healthy brain and serves as a marker for a range of neuropathological conditions. Non-invasive exploration of functional coupling using temporally resolved techniques such as MEG allows for a unique opportunity of exploring this fundamental brain mechanism in a reasonably ecological setting.The indirect nature of MEG measurements complicates the the estimation of functional coupling due to the spatial leakage effects. In previous work (Ossadtchi et al., 2018), we introduced PSIICOS, a method that for the first time allowed us to suppress the spatial leakage and yet retain information about functional networks whose nodes are coupled with close to zero or zero mutual phase lag.In this paper, we demonstrate analytically that the PSIICOS projection is optimal in achieving a controllable trade-off between suppressing mutual spatial leakage and retaining information about zero-phase coupled networks. We also derive an alternative solution using the regularization-based inverse of the mutual spatial leakage matrix and show its equivalence to the original PSIICOS. This approach allows us to incorporate the PSIICOS solution into the conventional source estimation framework. Instead of sources, the unknowns are the elementary networks and their activation timeseries are formalized by the corresponding source-space cross-spectral coefficients.Additionally, we outline potential avenues for future research to enhance functional coupling estimation and discuss alternative estimators that parallel the established source estimation approaches. Finally, we propose that the PSIICOS framework is well-suited for Bayesian techniques and offers a principled way to incorporate priors derived from structural connectivity.
Apical PAR-3 caps orient the mitotic spindle inC. elegansearly embryos
Authors: Stolpner Naomi J. , Manzi Nadia I. , Su Thomas , Dickinson Daniel J. 
Published: March 2023

AbstractDuring embryonic development, oriented cell divisions are important for patterned tissue growth and cell fate specification. Cell division orientation is controlled in part by asymmetrically localized polarity proteins, which establish functional domains of the cell membrane and interact with microtubule regulators to position the mitotic spindle. For example, in the 8-cell mouse embryo, apical polarity proteins form caps on the outside, contact-free surface of the embryo that position the mitotic spindle to execute asymmetric cell division. A similar radial or “inside-outside” polarity is established at an early stage in many other animal embryos, but in most cases it remains unclear how inside-outside polarity is established and how it influences downstream cell behaviors. Here, we explore inside-outside polarity inC. eleganssomatic blastomeres using spatiotemporally controlled protein degradation and live embryo imaging. We show that PAR polarity proteins, which form apical caps at the center of the contact free membrane, localize dynamically during the cell cycle and contribute to spindle orientation and proper cell positioning. Surprisingly, apical PAR-3 can form polarity caps independently of actomyosin flows and the small GTPase CDC-42, and can regulate spindle orientation in cooperation with the key polarity kinase aPKC. Together, our results reveal a role for apical polarity caps in regulating spindle orientation in symmetrically dividing cells and provide novel insights into how these structures are formed.
Unpacking the functional heterogeneity of the Emotional Face Matching Task: a normative modelling approach
Authors: Savage Hannah S. , Mulders Peter C. R. , van Eijndhoven Philip F. P. , van Oort Jasper , Tendolkar Indira , Vrijsen Janna N. , Beckmann Christian F. , Marquand Andre F. 
Published: March 2023

AbstractFunctional neuroimaging has contributed substantially to understanding brain function but is dominated by group analyses that index only a fraction of the variation in these data. It is increasingly clear that parsing the underlying heterogeneity is crucial to understand individual differences and the impact of different task manipulations. We estimate large-scale (N=7728) normative models of task-evoked activation during the Emotional Face Matching Task, which enables us to bind heterogeneous datasets to a common reference and dissect heterogeneity underlying group-level analyses. We apply this model to a heterogenous patient cohort, to map individual differences between patients with one or more mental health diagnoses relative to the reference cohort and determine multivariate associations with transdiagnostic symptom domains. For the face>shapes contrast, patients have a higher frequency of extreme deviations which are spatially heterogeneous. In contrast, normative models for faces>baseline have greater predictive value for individuals’ transdiagnostic functioning.
A numerical study on thermo-hydraulic performance of micro pin-fin heat sink using hybrid pin-fins arrangement for electronic cooling devices
Authors: Rezazad Bari Amir , Zabetian Targhi Mohammad , Heyhat Mohammad Mahdi 
Published: March 2023

Purpose
This study aims to examine the effect of a combination of hybrid pin-fin patterns on a heat sink's performance using numerical techniques. Also, flow characteristics have been studied, such as secondary flow formation and flow-wall interaction.

Design/methodology/approach
In this study, the effect of hybrid arrangements of elliptical and hexagonal pin-fins with different distribution percentages on flow characteristics and performance evaluation criteria in laminar flow was investigated. Ansys-Fluent software solves the governing equations using the finite volume method. Also, the accuracy of obtained results was compared with the experimental results of other similar papers.

Findings
The results of this study highlighted that hybrid arrangements show higher overall performance than single pin-fin patterns. Among the hybrid arrangements, case 3 has the highest values of performance evaluation criteria, that is, 1.84 in Re = 900. The results revealed that, with the instantaneous change in the pattern from elliptic to hexagonal, the secondary flow increases in the cross-sectional area of the channels, and the maximum velocity in the cross-section of the channel increases. The important advantages of case 3 are its highest overall performance and a lower chip surface temperature of up to about 2% than other hybrid patterns.

Originality/value
Prior research has shown that in the single pin-fin pattern, the cooling power at the end of the heat sink decreases with increasing fluid temperature. Also, a review of previous studies showed that existing papers had not investigated hybrid pin-fin patterns by considering the effect of changing distribution percentages on overall performance, which is the aim of this paper.
Low-carbon electricity technology transformation in Chinese universities
Authors: Wang Tianchong , Suo Baimin 
Published: March 2023

Purpose
With the growing climate problem, it has become a consensus to develop low-carbon technologies to reduce emissions. Electric industry is a major carbon-emitting industry, accounting for 35% of global carbon emissions. Universities, as an important patent application sector in China, promote their patent application and transformation to enhance Chinese technological innovation capability. This study aims to analyze low-carbon electricity technology transformation in Chinese universities.

Design/methodology/approach
This paper uses IncoPat to collect patent data. The trend of low-carbon electricity technology patent applications in Chinese universities, the status, patent technology distribution, patent transformation status and patent transformation path of valid patent is analyzed.

Findings
Low-carbon electricity technology in Chinese universities has been promoted, and the number of patents has shown rapid growth. Invention patents proportion is increasing, and the transformation has become increasingly active. Low-carbon electricity technology in Chinese universities is mainly concentrated in individual cooperative patent classification (CPC) classification numbers, and innovative technologies will be an important development for electric reduction.

Originality/value
This paper innovatively uses valid patents to study the development of low-carbon electricity technology in Chinese universities, and defines low-carbon technology patents by CPC patent classification system. A new attempt focuses on the development status and direction in low-carbon electricity technology in Chinese universities, and highlights the contribution of valid patents to patent value.
Corporate social responsibility in peer-to-peer accommodation: a focus on Airbnb
Authors: Farmaki Anna , Kladou Stella , Ioannides Dimitri 
Published: March 2023

Purpose
This paper aims to provide a critical synthesis of the interface of corporate social responsibility (CSR) and peer-to-peer (P2P) accommodation to offer insights that contribute to theory and practice of CSR in hospitality.

Design/methodology/approach
By using key CSR models, this paper reflects on the nexus between CSR and P2P accommodation (with a focus on Airbnb) to identify opportunities and challenges with regard to CSR implementation in P2P accommodation and, thereby, progress the research agenda on the topic.

Findings
This contribution will hopefully enable policymakers to improve the accountability of stakeholders related to P2P accommodation in terms of the sector’s impacts on local communities while contributing to the progression of the research agenda on CSR in hospitality.

Research limitations/implications
Because this contribution is meant to be a “critical reflection paper”, the main purpose is to flesh out a commentary offering recommendations on how to account for CSR in relation to P2P accommodation and primarily Airbnb. As such, this paper aims to prompt future empirical research on the topic. Naturally, the major downside of this type of paper is the lack of an empirical approach.

Practical implications
This paper advances theory on hospitality-related CSR, enabling policymakers to improve the stakeholders’ accountability related to P2P accommodation in terms of the sector’s impacts on local communities.

Originality/value
Despite the increasing importance of CSR in hospitality, minimal academic attention has been paid insofar to CSR in the P2P accommodation sector. This inattention is surprising given the rapid expansion of the sector which, in turn, has imposed significant pressures on local communities.
Interweaving resilience: leadership of continuous improvement partnerships and Mary Parker Follett's theory of relational process
Authors: Eddy-Spicer David H. 
Published: March 2023

PurposeFollett's relational process theory illuminates key aspects of interdependence among organizations in the field of education that are essential to fostering capacities for interorganizational resilience. The article argues for the necessity of developing mutualism in systems of education as essential preparation for times of instability and crisis, as demonstrated through recent experiences with the COVID-19 pandemic.Design/methodology/approachThis article provides an introduction to the relational process theory of Mary Parker Follett, a foundational theorist of organizing and administration. This theoretical review uses worked examples drawn from a collaborative, continuous improvement partnership focused on educational leadership preparation and development.FindingsThe author identifies four aspects of Follett's theory that connect most directly to collaborative, continuous improvement partnerships. These include mutualism as circular response, coactive power, embrace of difference through constructive conflict and integrating experience through learning. The article discusses how these offer an integrated framework of foundational concepts for nurturing and sustaining educational systems capable of adaptive change in the face of complex challenges.Originality/valueFollett's relational process theory offers a perspective on partnering as a dynamic and evolving constellation of interactions and activity. The implications of Follett's core ideas for education resonate beyond improvement partnerships and offer guidance at all levels of educational systems seeking to orient towards an evolutionary logic.
Women’s microbusiness participation decisions and their effect on poverty in the Wolaita zone, southern Ethiopia
Authors: Ergo Alemayehu Elda , O’Connor Deirdre , Leza Mega Tekle 
Published: March 2023

Purpose
Microbusinesses are better able to assist many disadvantaged groups in finding employment and breaking the cycle of poverty because they require less initial capital and employ a large number of poor people in developing economies. Women run and own the majority of micro-businesses in urban Ethiopia. This study aims to investigate women’s microbusiness participation decisions and the effect on poverty in the Wolaita zone southern Ethiopia.

Design/methodology/approach
A cross-sectional study was carried out using a mixed-methods research approach. A total of 384 women who owned micro-businesses were chosen using a systematic random sampling technique, while 36 women were purposefully chosen for qualitative data analysis. Data were gathered through survey questionnaires, in-depth interviews and focus group discussions. The collected data were analyzed by using a propensity score matching technique as well as contextual analysis.

Findings
The study discovered that women’s participation in registered micro-businesses had a higher and more beneficial impact on their food, non-food and overall consumer spending than women’s participation in unregistered microbusinesses, which helped to reduce poverty. Besides, overall women’s participation in micro-business increased their decision-making power and enabled them to provide resources for their families food and non-food consumption, with registered micro-business participants reaping the greatest benefits.

Originality/value
This research focused on the effects of women’s micro-entrepreneurship on poverty in low-income communities. Rather than providing food, clothing and/or other aid to women in disadvantaged communities, the authors asserted that assisting women and their micro-businesses allows them to be self-sufficient in terms of food and clothing as a long-term solution to poverty reduction. As a result, policymakers can use our findings to gain a better understanding of how women’s micro-entrepreneurship affects poverty reduction, allowing them to develop more effective anti-poverty initiatives. This study’s findings are novel and add to the body of knowledge in Ethiopia and the sub-Saharan African region.
Inclusive workplace practices: understanding disability-assistance animal stereotype-bias signals
Authors: McPherson Rebecca , Loafman Lucas Wayne 
Published: March 2023

Purpose
This study aims to fill a distinct gap in the literature on disability-assistance animals (disability-AAs) and inclusive employment by investigating human resource (HR) practitioners’ perceptions of disability-AAs in the staffing process and workplace. HR practitioners play a critical role in accommodation and inclusion, yet their experiences and insights have been largely ignored in prior research.

Design/methodology/approach
This study used a phenomenological approach, drawing on signaling theory and employability constructs, to explore insights from 17 HR practitioners’ experiences with assistance animals in the workplace.

Findings
The potential for unconscious bias in employment practices was found, as well as a significant percentage of practitioners who were unprepared to handle animal accommodations. First, the potential development of a positive stereotype bias suggests all genuine assistance animals are high functioning. Second, the assumption that employees’ assistance animal requests for invisible disabilities without previous disclosure are presumed fraudulent until proven valid.

Research limitations/implications
As a qualitative study, findings from this study are not generalizable to a larger population but may be transferable to similar employment contexts.

Originality/value
This study extends knowledge from previous studies, which focused predominately on insights from disabled individuals, animal trainers and therapists, to the HR practitioner domain in creating a more inclusive work environment. Findings from this study suggest the need to improve education about disability-AAs and the potential for unconscious bias for HR practitioners and hiring managers when accommodating requests, particularly when those assistance animals are not described as high functioning.
An instrument for studying TQM implementation in primary education: development and empirical investigation
Authors: Sfakianaki Eleni , Kaiseroglou Nikolaos , Kakouris Andreas 
Published: March 2023

Purpose
Primary education (PE) forms the foundation of high-quality education systems, but it is often underestimated. This paper aims to develop and empirically validate a measurement instrument to study the current stage of total quality management (TQM) implementation in PE.

Design/methodology/approach
This study developed a seven-dimensional and a 37-item instrument administered to education professionals at public PE institutions in Greece to determine the current stage of TQM implementation at their institution; 2,088 responses were received in total. Principal component analysis, confirmatory factor analysis and structural equation modeling were adopted to test the research instrument.

Findings
The findings supported the validity and reliability of the instrument and demonstrated that the proposed measures represent actual dimensions that can be used to explore the implementation of TQM in PE. Results also showed that the respondents rated highly the positive impact that TQM can have on their schools, although nonhuman resources have been found inadequate and problematic.

Practical implications
The field of TQM in PE lacks empirical evidence; this paper provides deep insight into the elements that demonstrate the perception and status of TQM implementation and can support quality implementation plans.

Originality/value
This study adds value to the TQM literature by developing an instrument for use in PE educational institutions and validating it in a real environment. Practitioners and researchers are thus supplied with the means to facilitate TQM practices, identify weaknesses and opportunities and contribute to social empowerment.