bluuebunny/crossref_metadata_2025_split huggingface dataset:
SCREENING FOR BIOMORPHOLOGICAL AND CYTOGENETIC FEATURES OF POLEMONIUM CAERULEUM L. AFTER COLCHICINE APPLICATION
Authors: Khazieva Firdaus M. , Basalaeva Irina V. , Konyaeva Elena A. , Burova Alla E. , Samatadze Tatyana Е. 
Published: August 2023

Background. Currently, it is quite relevant in breeding to create varieties with high yields of raw materials and the content of biologically active compounds.
This work aim is to identify promising forms of P. caeruleum L. is for further inclusion in the breeding process based on biomorphological, anatomical, cytogenetic studies and the bioproductivity of colchicine-induced and wild-growing forms in comparison with the Lazur variety.
Materials and methods. To obtain polyploids in 2018, the seeds of the P. caeruleum L. the Lazur variety were germinated in Petri dishes on filter paper at room temperature. When the roots reached the length of the seed, they were placed in different concentrations of colchicine solution 0.05 %, 0.1 %, 0.2 % and 0.52 % for 12 and 24 hours, then washed in running water for three hours.
Results. The article for the first time presents data on a comparative study of three forms of Polemonium caeruleum L. in terms of morphological characteristics, microscopy of the leaf epidermis and cytogenetic analysis. It was established that the raw materials productivity of the underground part of the plants tetraploid forms in the C1 generation significantly exceeded the diploid and wild forms of plants by 32 %, as well as the yield of seeds by 22 %. In the C2 generation, the yield of raw materials (rhizomes with roots) in tetraploid plants increases in relation to the control by 56 %, seeds by 34 %. Quantitative determination of the amount of triterpene saponins in terms of β-escin in the plant material of three forms of P. caeruleum L. showed an excess of this indicator in the colchicine form by 24 % in the grass and by 36 % in rhizomes with roots, in the wild-growing form - by 15 % in the aerial part and decrease in the underground part by 9 % in relation to the control. It was found that plants of colchicine and wild forms differ in the presence of larger stomata compared to the control. Based on the pattern of DAPI-bandin and the distribution of 45S rDNA and 5S rDNA genes, a generalized species idiogram of blue cyanosis was constructed, taking into account polymorphic variants of DAPI-bandin patterns and indicating the location of the sites of 45S rDNA and 5S rDNA genes. The cytogenetic stability of the resulting tetraploid form was revealed.
Conclusion. The article presents comparative studies on morphological characteristics, bioproductivity, microscopy of the leaf epidermis and cytogenetic analysis of the tetraploid form (colchicine) of P. caeruleum L. in comparison with the variety Lazur (diploid form) and the wild form of P. caeruleum L. plants.
GENDER FEATURES OF THE TEENAGE ATHLETES’ LIFE QUALITY AND PERSONAL ANXIETY DYNAMICS (BY THE HEALTH SCHOOL LESSONS RESULTS)
Authors: Khudonogov Igor Yu. , Pevneva Marina V. , Lyashenko Kristina N. 
Published: August 2023

Relevance. This work is devoted to social diagnostics and social prevention of possible negative side effects of medical, social and psychological plan that occur against the background of the main training and educational processes of pubertal athletes, taking into account their gender.
Purpose: to assess the gender characteristics of the life quality and personal anxiety (PA) dynamics of the Brothers Samurgashevs’ Rostov Region Olympic Reserve Sports School No. 35 high school students against the background of classes at the health school (HS).
Methods: historical, pedagogical, socio-psychological, social prevention, sociological, statistical and graphic methods, as well as the method of content analysis.
Results: after the HS classes PA decreased more than 40% overall for the sample. The decrease in PA was accompanied by an increase in QoL by almost 15%. The main PA priorities for female athletes turned out to be the following signs: “You are in danger of failure, bad luck”; “To see bad or “prophetic” dreams”; “Something is not working out for you”. Among male athletes, the initial level of PA was lower than that of female by 40.1%. The priorities of male anxiety were such signs as: “You are in danger of failure, bad luck”; “They don’t pay attention to you”; “You can’t do your homework”. After HS classes, girls most quickly eliminated PA in such areas as “Waiting for parents after their personal conversation with the school principal” and “Seeing bad or “prophetic” dreams”, and boys - “You don’t understand the teacher’s explanations”, “(They) fell silent when you approached”, “Something is not working out for you”. The initial level of QoL of young female athletes was significantly lower by 8.7% than that of young male athletes, and after training at the HS, it exceeded the corresponding indicator in the group of young male athletes by 4.7%. After classes at the HS, young male athletes noted an improvement in relations during their studies (with peers, superiors, etc.), however, in quantitative terms, this improvement was 9 times lower than in the group of young female athletes. Also, young male athletes noted an improvement in their physical condition and strengthening of skills and abilities in organizing their time, but 5 and 4 times (respectively) less pronounced than in the group of young female athletes.
Conclusion. The results obtained can be widely disseminated in similar sports schools to ensure monitoring and bringing the level of personal anxiety and quality of life of students to age norms.
CHARACTERISTICS OF THE RELATIONSHIP BETWEEN THE EXPERIENCE OF TIME AND THE LEVEL OF NEURO-MENTAL STRESS IN MILITARY UNIVERSITY CADETS
Authors: Yanitsky Mikhail S. , Utyuganov Alexey A. , Ivanov Mihail S. , Frolova Larisa V. 
Published: August 2023

Background. The article discusses the problem of psychological health safety of military university cadets, defined in the study through the concept of neuro-psychic tension. The content of the cadets’ attitude to time as a personal correlate of psychological health is analyzed.
Materials and methods. To study the attitude to time, Wasserman’s «Semantic Time Differential» and F. Zimbardo ZTPI techniques are used. To measure the level of neuro-psychic tension, an abbreviated version of the MMPI technique and a method for measuring the level of neuropsychic stability are used. Statistical methods (Mann-Whitney comparative analysis, cluster analysis) were used in the processing. The study was conducted at the Novosibirsk Military Institute of the National Guard Troops of the Russian Federation on a sample of 172 cadets.
Results. The study shows statistically significant differences in the characteristics of the time perspective and the semantic content of the attitude to time between cadets with different levels of neuro-psychic tension.
Conclusion. It is concluded that there is a nonlinear relationship between the studied characteristics according to the principle of «optimum»: the optimal indicators of the attitude to time correspond to the normal (average) level of neuro-psychic tension.
CHANGE CHARACTERISTICS IN SALIVA AND FECES MICROBIOTA OF A DESMINOPATHY T341P PATIENT
Authors: Pauls Viacheslav Yu. 
Published: August 2023

Background. A rare muscular disease, desminopathy, is caused by mutations in the DES gene. At present, information about changes in the microbiota of biological media present in such patients is very scarce.
Purpose. The aim of the study is to study changes in the saliva and feces microbiota of patients with desminopathy T341P in a heterozygous state.
Materials and methods. The retrospective investigation comprised observation of the observation of a proband with the family form of desminopathy T341P. 56 clinically significant microorganisms were numerically analyzed immediately in the obtained biological material by gas chromatography-mass spectrometry method.
Results. The emergence of Epstein-Barr viruses, Cytomegalovirus, Herpes spp and gram-negative rods was noted in the proband’s biological media under investigation during the desminopathy progression. Excessive bacterial growth of fecal microbiota was observed along with a decrease in saliva microorganisms. There is an excess of the norm in the total number of microorganisms and an increase in their species diversity. Propionibacterium jensenii, Eubacterium spp and Eggerthella lenta predominated in the feces and Clostridium ramosum – in the saliva. An increase in fecal microbiota transient with Peptostreptococcus anaerobius 18623 dominance was observed along with the emergence and rapid 441-time growth of potentially dangerous bacterium Clostridium difficile. The total level of endotoxin in the proband’s saliva and fecal microbiota increases to exceed the norm in 13.7 and 81.8 times, respectively. At the same time, a low level of plasmalogen was noted.
Conclusion. The investigation results can be useful for developing complex intervention tactics.
IMPACTS OF CLIMATE CHANGE, FORMS, AND EXCESS OF NITROGEN FERTILIZERS ON THE DEVELOPMENT OF WHEAT FUNGAL DISEASES
Authors: Diakite Simbo , Pakina Elena N. , Behzad Abdullah , Zargar Meisam , Saquee Francess Sia , Kalabashkina Elena V. , Tsymbalova Vitaliya A. , Astarkhanova Tamara S. 
Published: August 2023

Background. Global climate change and excessive nitrogen application has become a significant issue and inevitably threatens sustainable wheat production, not only with direct negative effects on crop growth but also with profound impacts on biology and pest and disease management.
Purpose. This review addresses the current challenges, namely the negative effects of climate change and the forms and excess of nitrogen-rich fertilizers on the development of fungal diseases in wheat, as well as management strategies.
Materials and methods. To achieve the stated objective of the study, the scientific literature published during the last 20 years on the impacts of climate change and the forms and excesses of nitrogen fertilizers on the development of fungal diseases and on the yield of wheat were reviewed.
Results. Thus, in mitigating these challenges, it is necessary to optimize the dose of nitrogen fertilizers, apply nitrogen in the form of nitrate, ammonium sulphate, ammonium nitrate, and coated urea fertilizers, to use silicate fertilizers such as calcium, magnesium, and potassium silicate, and to perform a long rotation of wheat through perennial legumes and leguminous crops, as well as to develop, through genome editing, varieties with high yield potential, resistant to biotic and abiotic stresses, and of good end-use quality, or plant new cereals that have needs for heat and a longer reproductive growth period.
Conclusion. To develop an effective agricultural management strategy, future research should be based on the study of the interactions among crops, pests, pathogens and farming system under climate change, taking into account all parameters such as temperature increase and CO2, extreme precipitation, etc. A sufficient number of results must be published to be able to draw meaningful conclusions.
CHOLINE PREPARATIONS IN THE TREATMENT OF ASTHENIC STATES: MODERNITY AND PERSPECTIVES (A LITERATURE REVIEW WITH A CLINICAL CASE PRESENTATION)
Authors: Magomedova Kamila Sh. , Bykov Yuriy V. , Bekker Roman A. 
Published: August 2023

Purpose. To provide the reader with a detailed description of the biochemical and physiological effects of choline, which make it an indispensable component of our daily food. Then to thoroughly review all the relevant clinical data on the use of choline preparations for the treatment of asthenic and astheno-depressive conditions and cognitive impairments. Then, to present the reader with a clinical case from our own practice, illustrating the effectiveness of the use of choline alfoscerate as part of the combination therapy of a mild form of post-COVID astheno-depressive syndrome in a 19-year-old man who have refused to take antidepressants, as well as any other psychotropic drugs, but instead wanted some form of pharmacotherapy based exclusively on «natural components».
Methodology. We have searched PubMed, Google Scholar, Web of Science databases for keywords such as «choline biochemistry», «choline and phospholipids», «choline and betaine», «arsenolipids», «nitrolipids», «choline and cognition», «choline and asthenia». Then we filtered the data we had found by their relevance. Then we summarized and presented the relevant data in the theoretical part of this article. Then we described in detail the clinical case of the effective use of choline alfoscerate in the complex therapy of post-COVID asthenic-depressive state which we have encountered in our own practice.
Results. The results we have obtained during compilation of this review, in our opinion, may allow us to draw preliminary conclusions about the possible prospects for the use of choline preparations (and, in particular, choline alfoscerate preparations) in monotherapy or as part of combination therapy of asthenic and / or astheno-depressive states (especially if those states are accompanied by impaired memory, concentration, increased mental fatigue), including those states that were caused by a recent history of COVID-19 (that is, asthenic or astheno-depressive states developed as part of the post-covid syndrome).
To be able to make any further, more firm conclusions regarding the efficacy and safety of the use of choline-containing preparations in this context, it will be necessary to organize and conduct large, multicenter, methodologically sound, double-blind, placebo-controlled, randomized clinical trials (RCTs), with clinically meaningful and relevant outcomes in the treatment of asthenia clearly defined as trial endpoints in advance (before starting the trial itself).
Practical implications. We believe that the results we have presented in this review justify the need to further study the efficacy and safety of the use of choline preparations, both as monotherapy and as a part of combination therapy of asthenic and astheno-depressive conditions, preferably in the format of large, multicenter, well organized, methodologically sound, double-blind, placebo-controlled RCTs with clearly pre-defined clinically meaningful and relevant endpoints.
Rising Temperatures, Falling Ratings: The Effect of Climate Change on Sovereign Creditworthiness
Authors: Klusak Patrycja , Agarwala Matthew , Burke Matt , Kraemer Moritz , Mohaddes Kamiar 
Published: August 2023

Enthusiasm for “greening the financial system” is welcome, but a fundamental challenge remains: financial decision makers lack the necessary information. It is not enough to know that climate change is bad. Markets need credible, digestible information on how climate change translates into material risks. To bridge the gap between climate science and real-world financial indicators, we simulate the effect of climate change on sovereign credit ratings for 109 countries, creating the world’s first climate-adjusted sovereign credit rating. Under various warming scenarios, we find evidence of climate-induced sovereign downgrades as early as 2030, increasing in intensity and across more countries over the century. We find strong evidence that stringent climate policy consistent with limiting warming to below 2 °C, honoring the Paris Climate Agreement and following representative concentration pathway (RCP) 2.6, could nearly eliminate the effect of climate change on ratings. In contrast, under higher emissions scenarios (i.e., RCP 8.5), 59 sovereigns experience climate-induced downgrades by 2030, with an average reduction of 0.68 notches, rising to 81 sovereigns facing an average downgrade of 2.18 notches by 2100. We calculate the effect of climate-induced sovereign downgrades on the cost of corporate and sovereign debt. Across the sample, climate change could increase the annual interest payments on sovereign debt by US$45–$67 billion under RCP 2.6, rising to US$135–$203 billion under RCP 8.5. The additional cost to corporations is US$10–$17 billion under RCP 2.6 and US$35–$61 billion under RCP 8.5. This paper was accepted by Colin Mayer, Special Section of Management Science on Business and Climate Change. Funding: M. Agarwala, P. Klusak, and M. Burke acknowledge funding from the International Network for Sustainable Financial Policy Insights, Research and Exchange (INSPIRE). M. Agarwala also acknowledges funding from The Wealth Economy Project. Supplemental Material: The data files and online appendix are available at https://doi.org/10.1287/mnsc.2023.4869 .
NO CLINICAL ADVANTAGE WITH CUSTOMIZED INDIVIDUALLY MADE IMPLANTS OVER CONVENTIONAL OFF-THE-SHELF IMPLANTS IN TOTAL KNEE ARTHROPLASTY: A SYSTEMATIC REVIEW AND META-ANALYSIS
Authors: Khaleeq Tahir , Saeed Abu Zer , Ahmed Usman , Ajula Randeep , Boutefnouchet Tarek , D'Alessandro Peter , Malik Shahbaz 
Published: August 2023

AbstractCustomised individually made implants(CIM) total knee arthroplasty(TKA) are custom-made to better fit patients native anatomy and aim to improve outcomes which can be variable with conventional off-the-shelf(OTS).A systematic review and meta-analysis was conducted searching the MEDLINE and Embase databases. Studies reporting on patient reported outcome measures, clinical or radiological outcomes were included.23 studies satisfied the search criteria (case-control studies14, case series8, cross-sectional studies1). There were 2,856(CIM) and 1,877(OTS) implants. The overall revision-rate was higher in CIM 5.9%vs3.7%OTS [OR 1.46(95% CI 0.82–2.62)]. MUA was higher in the CIM group 2.2%vs.1.1%OTS [OR 2.95(95% CI 0.95–9.13)] and overall complications rate was also higher in the CIM group 5% vs. 4.5%OTS [OR 1.45(95% CI 0.53–3.96)]. LOS was significantly shorter in the CIM group 2.9 days vs. 3.5 days [MD −0.51(95% CI −0.82–0.20)]. Pooled analysis for KSS showed no difference between CIM and OTS groups(Knee=90.5 vs. 90.6 [MD-0.27,(95% CI −4.27–3.73)] and Function=86.1 vs. 90.6[MD 1.51 (95% CI −3.69–6.70)] component of the scores. There was no significant difference in post-operative ROM between CIM and OTS groups 117.3° vs. 115.0° [MD 0.02,(95% CI −1.70–1.74)].CIM TKAs has theoretical benefits over OTS TKAs however in this review they were associated with higher complication, MUA and revision rates with no difference in outcome scores and no improvement in target alignment. The findings of this review does not support the use of CIM over OTS prosthesis in total knee arthroplasty.
EVALUATING BASIC KNEE ARTHROSCOPY SKILLS IN ORTHOPAEDIC TRAINEES IN A LIMITED RESOURCE SETTING
Authors: Berry Kirsty , Von Bormann Richard , Roche Stephen , Laubscher Maritz , McCollum Graham , Held Michael 
Published: August 2023

AbstractBackgroundOrthopaedic training in Southern Africa is largely focused on trauma, although elective procedures, such as knee arthroscopy are increasing. This is especially true in the private sector where most trainees will practice. The primary aim of this study was to assess the arthroscopic competency of orthopaedic trainees in a setting of limited resources.MethodsA prospective observational cohort study was carried out. Orthopaedic trainees of a Southern African university hospital performed basic arthroscopy on a knee model. Their surgical competency was assessed by two surgeons proficient in arthroscopy using the modified Basic Knee Arthroscopy Skill Scoring System (mBAKSSS).ResultsA total of 16 trainees (12 male) were included (6 junior and 10 senior trainees). The median age of participants was 36 (34.8, 37). The median mBAKSSS was 28.0 (20.3, 32.5) but showed a large variability (12.0–42.5). The overall reliability was excellent with Cronbach's Alpha of 0.91 and interclass correlation of 0.91 [95% CI 0.75, 0.97].ConclusionsThe average knee arthroscopy proficiency of our trainees is comparable to those of international training programs, but there was great variability with inconsistent skills amongst the trainees. This calls for improved and reproducible arthroscopy training and skills transfer, exposure to procedures and ongoing assessment.
CEMENTING TECHNIQUES IN KNEE SURGERY (CETIKS): A UK EXPERT CONSENSUS STUDY
Authors: Hampton Matthew , Balachandar Vivek , Charalambous Charalambos , Sutton Paul 
Published: August 2023

AbstractIntroductionAseptic loosening is the most common cause of failure following cemented total knee arthroplasty (TKA) and this has been linked to poor cementation technique. We aimed to develop a consensus on the optimal technique for component cementation in TKA.MethodologyA UK based, three round, online modified Delphi Expert Consensus Study was completed focussing on cementation technique in TKA. Experts were identified as having a minimum of 5 years Consultant experience in the NHS and fulfilling any one of the following three criteria:· A ‘high volume’ knee arthroplasty practice (>150 TKA per annum) as identified from the National joint registry (NJR).· A senior author of at least 5 peer reviewed articles related to TKA in the previous 5 years· A named trainer for a post CCT fellowship in TKA.ResultsEighty-one experts (Round 1) and eighty experts (Round 2 and 3) completed the Delphi Study. Four domains with a total of twenty-four statements were identified. 100% consensus was reached within the cement preparation, pressurisation, and cement curing domains. 90% consensus was reached within the cement application domain. Consensus was not reached with only one statement regarding the handling of cement during initial application to the tibial and/or femoral bone surfacesConclusionThe CeTIKS expert consensus study presents comprehensive recommendations on the optimal technique for component cementation in TKA. Expert opinion has a place in the hierarchy of evidence and until better evidence is available these recommendations should be considered when cementing a TKA.
ANTERIOR CRUCIATE LIGAMENT INJURY PREVENTION PROGRAMMES IN WELSH NETBALL: SURVEY OF KNOWLEDGE, IMPLEMENTATION, AND BARRIERS AMONG PLAYERS AND COACHES
Authors: Abdul Wahid , Moore Isabel , Robertson Angus 
Published: August 2023

AbstractIntroductionPerception of ACL injury prevention programs amongst professional netball players and coaches has not been studied. We investigated (1) level of awareness and experience of ACL injury prevention programs; (2) use of ACL injury prevention programs; and (3) barriers to implementing ACL injury prevention program in netball.MethodologyFemale netball players representing Welsh senior and under-21 teams and elite and amateur coaches were invited electronically to this web-based study between 1st May–31st July 2021. Information on ACL injury susceptibility and seriousness, knowledge, experience, and implementation of ACL injury prevention programs were ascertained.ResultsTwenty-eight players (77.8%) and 29 coaches (13.2%) completed the questionnaire. Seventeen (60.7%) players and 15 (51.7%) coaches reported female athletes were at greater risk for sustaining ACL injuries. Over 90% of respondents identified netball as high-risk, whilst 89% of players and 76% of coaches reported these injuries to be preventable. Two (7.1%) players and 6 (20.7%) coaches utilised ACL injury prevention programs with lack of time and engagement from coaches and players identified. Majority of respondents indicated that their club has neither promoted, advocated nor demonstrated exercises for ACL injury prevention. Over 90% of respondents would utilise such programs if it minimised players risk with appropriate training and information.ConclusionStudy highlights limited knowledge of female athletes’ increased susceptibility of ACL injuries with lack of communication and education of ACL injury prevention programs between sporting associations, coaches and players. Results demonstrate willingness of players and coaches to implement ACL injury prevention programs in Welsh netball.