bluuebunny/crossref_metadata_2025_split huggingface dataset:
Mechanistic Study of Long Non-Coding RNA SNHG3 in Promoting Prostate Cancer Proliferation and Invasion
Authors: Shi Zebin , Sun Heyun , Zhang Wei , Zhuang Wenyuan , Shi Xiaokai , Zhang Lei , Gao Shenglin , Zhang Lifeng 
Published: February 2025

IntroductionSNHG3 (small nucleolar RNA host gene 3) is a long non-coding RNA (lncRNA) that plays a pivotal role in cellular regulation. It is intricately involved in modulating the cell cycle, enhancing cell proliferation, and inhibiting apoptosis. These functions position SNHG3 as a significant contributor to oncogenic processes. Given its substantial influence on tumorigenesis, elucidating the exact molecular mechanisms through which SNHG3 operates is paramount. Comprehensive understanding of SNHG3's interactions and pathways is not only critical for advancing our knowledge of cancer biology but also holds immense potential for identifying novel therapeutic targets. By targeting the regulatory networks associated with SNHG3, new strategies can be developed for the effective treatment of malignancies where SNHG3 is implicated.Material and methodsWe investigated the role of lncRNA SNHG3 in promoting prostate cancer (PCa) proliferation and invasion. Using TCGA data, we assessed SNHG3 expression in cancer/adjacent tissue, Gleason score, biochemical recurrence, and overall survival. SNHG3 expression was manipulated in four cell types via siRNA interference/over-expression. Various experiments were conducted to confirm SNHG3's role in PCa, including clone ability, apoptosis, migration, Transwell invasion, and subcutaneous tumor experiments in nude mice.ResultsIn PCa, SNHG3 was highly expressed, especially in Gleason score >7 patients, correlating with shorter overall survival. Interfering with SNHG3 using a plasmid reduced proliferation, increased apoptosis, and decreased migration and tumor growth. Conversely, SNHG3 overexpression yielded opposite results.ConclusionsThe lncRNA SNHG3 promotes PCa proliferation and invasion while inhibiting cell apoptosis.
Unveiling the Immune Related ceRNA Network in Osteoarthritis: Key Biomarkers and Therapeutic Targets
Authors: Shi Jia-qi , Chen Li , Wang Xu , Ma Xin 
Published: February 2025

IntroductionThe aim of this study was to explore the immune-related competitive endogenous RNA (ceRNA) network in osteoarthritis (OA), focusing on identifying differentially expressed long non-coding RNAs (lncRNAs), constructing a classification model, and uncovering the associations between these lncRNAs and immune cell subsets in OA.Material and methodsMicroarray data from the Gene Expression Omnibus (GEO) database was used to identify differentially expressed genes (DEGs) in synovial tissue of OA. A classification model was constructed using the Least Absolute Shrinkage and Selection Operator (LASSO) regression with selected lncRNAs. Computational methods like CIBERSORT were employed to quantify immune cell infiltration patterns, and weighted gene co-expression network analysis (WGCNA) was conducted to delineate co-expression modules. Finally, a ceRNA network was constructed to elucidate the regulatory interactions among lncRNAs, miRNAs, and mRNAs.ResultsWe identified 5927 DEGs, among which 47 were differentially expressed long non-coding RNAs (DELs). Seven DELs (DGCR11, FAM215A, HCG9, PART1, FAM106A, NOP14-AS1, PRORY) formed the basis of a classification model with an area under the receiver operating characteristic (ROC) curve of 1. We also examined the infiltration patterns of immune cells in OA tissues and found significant differences compared to healthy controls, indicating a strong immunological component in OA pathogenesis. WGCNA identified a turquoise module with a high correlation to OA traits.ConclusionsThe study highlights the importance of ceRNA networks in understanding the complex pathogenesis of OA and offers a comprehensive framework for future research into potential diagnostic biomarkers and therapeutic targets.
Survival analysis of antibiotics in patients undergoing cardiopulmonary bypass in the intensive care unit: A study based on the Medical Information Mart for Intensive Care-IV database
Authors: Ma Xian , He Jie , Liu Jiangmin , Zi Congna 
Published: February 2025

IntroductionThis project was designed to evaluate the influence of antibiotics on the survival of patients in the intensive care unit (ICU) undergoing cardiopulmonary bypass (CPB) treatment.Material and methodsThis retrospective cohort study included data of 7,296 patients who underwent CPB surgery and were admitted to the ICU from MIMIC-IV database. Patients with CPB were grouped according to their survival time of more than 30 days or less after admission and whether antibiotics were used, with baseline characteristics analyzed. Survival differences were demonstrated by utilizing Kaplan-Meier (K-M) curves.ResultsIn CPB patients grouped according to survival time, great differences were detected in laboratory indexes, comorbidities, and treatment information. In terms of disease severity scores, vital signs, and comorbidity, there were notable differences in the data in CPB patients grouped by whether antibiotics were administrated. K-M curves manifested that the use of antibiotics substantially increased the 30-day survival rate of all CPB patients as well as CPB patients without sepsis complications. Landmark analysis indicated that the use of antibiotics greatly heightened the survival rates of all CPB patients and CPB patients without sepsis complications at 7 and 14 days after ICU admission.ConclusionsIn CPB patients admitted to the ICU, the rational use of antibiotics for treatment and prophylaxis can remarkably minimize the risk of patient mortality. These findings proffer essential references for clinical practice, assisting healthcare professionals to better assess and manage CPB patients in the ICU and formulate appropriate treatment plans to improve patient survival rates.
Withaferin-A Inhibits Colorectal Cancer Growth and Metastasis By Targeting the HSP90/HIF-1α/EMT axis
Authors: Lu Wen-qi , Li Xiang-de , Gu Yunman , Huang Jiang-ming , Qin Yan-kai , Cai Xiao-yong , Wang Chunming 
Published: February 2025

IntroductionWithaferin-A (WA) derived from natural products can inhibit the growth and metastasis of colorectal cancer (CRC) in vitro and in vivo.Material and methodsTranscriptome sequencing showed that the WA inhibited the epithelial-mesenchymal transition pathway (EMT), HSP90, and HIF-1α genes of CRC, and further experiments showed that WA inhibited HSP90, HIF-1α, E-Cadherin, and Co-IP, signifying that WA inhibits the protein interaction between HSP90 and HIF-1α. Knockdown of HIF-1α can reduce the migration and invasion capabilities of HCT116 and SW480 cells, and in vivo experiments have proved that HIF-1α knockdown SW480 lung metastasis cells have significantly reduced in numbers compared to the control group.ResultsThe classic HSP90 inhibitor, 17-AAG, can inhibit the migration and invasion of HCT116 and SW480 cells. We performed Q-PCR and western blot and found that 17-AAG can inhibit HSP90 and HIF-1α of CRC. The overexpression of HSP90 on HIF-1α knockdown SW480 cells can reinstate the migration and invasion ability, HIF-1α protein expression level, and protein binding ability of HSP90 and HIF-1α in SW480 cells.ConclusionsThese findings indicate that WA inhibits CRC's growth, migration, and invasion by inhibiting the HSP90/HIF-1α/EMT axis. Our result showed that WA may be one of the potential targets for the management and eventual cure of CRC.
The protective effect of sodium-glucose cotransporter 2 inhibitors and the incidence of ovarian cancer: a nationwide cohort preliminary study
Authors: Ting Ke-Hsin , Huang Jing-Yang , Liao Pei-Lun , Lee Chia-Yi , Yang Shun-Fa 
Published: February 2025

IntroductionSodium-glucose cotransporter 2 (SGLT2) inhibitors are commonly used antihyperglycemic medications that also exhibit anti-inflammatory and antioxidant effects. Ovarian cancer, a common gynecological neoplasm, is associated with increased inflammation and oxidative stress. Thus, this study investigated the correlation between the usage of SGLT2 inhibitors and the incidence of ovarian cancer in the population with type 2 diabetes mellitus (T2DM).Material and methodsA retrospective cohort study was conducted, and patients with T2DM were divided into those who used SGLT2 inhibitors and those who did not. A total of 163 668 and 327 336 patients with T2DM were categorized into the SGLT2 inhibitor and control groups, respectively. The primary outcome was the development of ovarian cancer, as identified using diagnostic codes and laboratory examination findings. Cox proportional hazard regression was adopted to yield the adjusted hazard ratios (aHRs) with 95% confidence intervals (CIs) for ovarian cancer events between the two groups.ResultsA total of 167 and 222 patients developed ovarian cancer in the SGLT2 inhibitor and control groups, respectively. The incidence of ovarian cancer was significantly lower in the SGLT2 inhibitor group than in the control group (adjusted hazard ratio: 0.73, 95% CI: 0.60-0.89, P = 0.0023). Subgroup analysis stratified by oral medications revealed that the effect of SGLT2 inhibitors on ovarian cancer development was significantly different from those of biguanides, sulfonylureas, alpha-glucosidase inhibitors, and dipeptidyl peptidase-4 inhibitors (P < 0.05).ConclusionsThis preliminary study showed that the administration of SGLT2 inhibitors in patients with T2DM is associated with a lower incidence of ovarian cancer.
Facts and myths about use and effect of statins in patients with dyslipidaemia - a survey of physicians.
Authors: Hendzel Agata , Krzyżanowski Filip , Kłoda Karolina , Mastalerz-Migas Agnieszka , Babicki Mateusz 
Published: February 2025

IntroductionStatins are the primary medications used to treat lipid disorders. However, numerous myths surrounding statin therapy lead to patient non-adherence and therapy discontinuation. The aim of this study was to assess the most common patient concerns regarding statin use.Material and methodsA survey was conducted targeting doctors using an online questionnaire The first section included questions regarding socio-economic status, while the second focused on patient experiences related to refusing statin treatment due to fears of specific side effects, encounters with side effects during therapy, and estimates of the percentage of patients who discontinue treatment. The concluding section addressed the most common reasons for treatment termination from the perspective of practitioners, along with efforts to educate their patients.Results260 questionnaires were collected. Notably, 84% and 81% of doctors reported encountering refusals of statin treatment due to patients’ fears of liver and muscle damage, respectively. The majority of respondents indicated that 10-20% of patients discontinue treatment on their own, despite significant side effects occurring in less than 10% of cases. Muscular symptoms were cited as the reason for discontinuing therapy in 75% of cases, while misinformation regarding statin side effects contributed to 53% of discontinuations. Additionally, 96.5% of doctors acknowledged efforts to educate their patients about statins.ConclusionsMany harmful beliefs about the side effects of statins persist among patients, resulting in non-adherence to treatment. The most prevalent concerns involve fears related to muscle and liver damage. These issues can be mitigated through targeted education for both patients and healthcare professionals.
A Causal Inference Study on the Impact of Asthma on the Onset of Chronic Obstructive Pulmonary Disease: Two-Sample Mendelian Randomization
Authors: Liu Wusheng , XU YuHui , Zhu Zanlei , Zhang ShiHao 
Published: February 2025

IntroductionRespiratory diseases have long been a focus of research in the field of public health, posing a serious threat to the health of the global population ，Asthma and COPD (chronic obstructive pulmonary disease) are two common respiratory ailments with overlapping pathogenesis.Material and methodsIn this study, utilizing a two-sample Mendelian randomization (MR) approach and analyzing publicly available genome-wide association study (GWAS) datasets, we explored the causal impact of asthma on the onset of COPD. Using genetic instrumental variables associated with asthma (p < 5 × 10⁻⁸) and applying multiple MR methods (IVW, MR-Egger, and weighted median), we identified a significant causal relationship between asthma and COPD. The inverse variance weighted (IVW) method indicated that asthma increases the risk of developing COPD with an odds ratio (OR) of 1.35 (95% confidence interval [CI]: 1.12–1.58, p = 0.002).ResultsAdditionally, multivariable MR analysis was performed to account for potential confounders, such as eosinophil count, smoking, and falls, which demonstrated that the association remains significant even after adjusting for these factors (OR: 1.29, 95% CI: 1.08–1.50, p = 0.004).ConclusionsThis study provides robust evidence supporting the causal link between asthma and COPD, offering a more comprehensive understanding of their relationship.
Keywords: Asthma, GWAS, Mendelian Randomization, Causal Relationship, COPD, Environmental Exposure Factors
Low Back Pain among Medical Students: A Cross-Sectional Study from Poland
Authors: Bałoniak Andrzej , Stępka Jan , Woźna Julia , Łapaj Łukasz , Chodór Paweł , Owecki Maciej 
Published: February 2025

IntroductionLow back pain (LBP) is a common condition and the leading cause of disability worldwide. Medical students may be at higher risk due to prolonged sitting and stress. The aim of this study was to examine the prevalence of LBP and disability among Polish medical students and identify associated risk factors.Material and methodsIn this study, 533 medical students, predominantly female (66.4%) with a median age of 22, were surveyed via an online questionnaire. The survey used the Oswestry Disability Index (ODI) and Patient Health Questionnaire (PHQ-9) to evaluate LBP and depression symptoms. Demographic data and information on smoking, exercise, sitting duration, spine posture, past and present LBP, pain duration, intensity, and analgesic use were also collected.ResultsPoint prevalence LBP was 70.95% while 78.26% of participants reported past history of LBP. The median LBP intensity measured with Numerical Rating Scale (NRS) was 3 and median ODI score was 10%. Overall female medical students suffered more from LBP than males (66.4% vs 33.6%). Following LBP risk factors were identified in the studied group: past episodes of LBP (OR: 2.92), sitting 8 hours per day (OR: 2.44), as well as 10 hours or more (OR: 2.95) moderate and severe depression symptoms (OR: 2.51, OR: 7.33 respectively).ConclusionsPrevalence of low back pain among medical students in the studied group is high, resulting in mild disability, with females experiencing more severe symptoms than males. Past LBP episodes, sitting 8 hours or more, and depression symptoms are independent risk factors for developing LBP among Polish medical students.
Trends in stroke prevalence caused by an elevated BMI in the European population
Authors: Tao Mengjun , Wu Yang , Cao Huiru , Peng Hui 
Published: February 2025

IntroductionThis study investigated the complex relationship between an elevated body mass index (BMI) and stroke risk in Europe amidst rising obesity rates and an aging population.Material and methodsA joinpoint model was used to fit the data on stroke mortality rates attributed to an elevated BMI. The age period cohort model was used to analyze the effects of age, period, and birth cohort on trends in stroke prevalence related to an elevated BMI from 1990–2019 in the European context. A Bayesian model was used to predict stroke mortality from increased BMI in Europe in 2020-2044.ResultsStroke mortality rates attributed to an elevated BMI displayed a wave-like declining trend in Europe, decreasing from 21.73/100,000 in 1990 to 14.01/100,000 in 2019. The most substantial decline in stroke mortality rate occurred between 2003 and 2013 ( -4.10%，P<0.001). Male mortality rates decreased from 21.48/100,000 in 1990 to 15.45/100,000 in 2019, while female rates declined more significantly from 21.40/100,000 in 1990 to 12.48/100,000 in 2019. Age-standardised stroke mortality due to increased BMI is expected to decline in Europe over the next 25 years.ConclusionsFrom 1990 to 2019, stroke mortality rates associated with elevated BMI in Europe steadily increased. However, projections indicate that this trend may shift, with a slight decline expected in the near future. Given these patterns, there is an urgent need in Europe to enhance weight management strategies for stroke patients and increase public health awareness, particularly focusing on men and the elderly, to help reduce stroke-related mortality.
Interleukin 6: A Friend or Foe in Diabetic Nephropathy
Authors: Alhamawi Renad , Mohammedsaeed Walaa , Aljumaa Maha 
Published: February 2025

IntroductionThis study aimed to investigate the relationship between serum cytokine levels, particularly Interleukin 6 (IL-6), and diabetic nephropathy (DN) in patients with type 1 (T1D) and type 2 diabetes (T2D).Material and methodsA cross-sectional study was conducted among 200 patients diagnosed with either T1D or T2D from January 2022 to December 2023 at the endocrinology and diabetes department of Madinah Hospital, Saudi Arabia.ResultsA total of 200 individuals with type T1D (n=100) or T2D (n=100) were enrolled in this research. Male patients (54%) and those aged 30–50 (67.5%) dominated the cohort. About 50% had diabetes for less than 10 years, and 49.5% were overweight. 63.5% had reduced glomerular filtration rate, whereas 46% (n=92) had albuminuria. T1D patients were mostly normal weight, while T2D patients were overweight or obese. Both diabetes types had identical kidney damage stages. T1D patients are more likely to have moderately elevated albuminuria (53% vs. 37%, p <0.05) than T2D individuals. T1D patients had considerably lower serum cytokine levels than T2D patients. IL-6 levels were moderately correlated with fasting blood glucose (r = -0.318, p<0.01) and HbA1c (r= -0.319, p<0.01) in T1D patients. IL-6 had a modest correlation with renal dysfunction markers, including GFR and urine albumin-creatinine ratio (r= -0.250, p<0.05 and r= 0.338, p<0.001, respectively).ConclusionsLower serum IL-6 levels in T1D patients are linked to delayed onset of kidney damage. The pro-inflammatory role of IL-6 may contribute to the development of diabetic nephropathy in T1D patients, as indicated by its association with albuminuria and renal function markers.
EFISIENSI BIAYA TRANSPORTASI MELALUI OPTIMASI RUTE: PENDEKATAN SAVING MATRIX DAN NEAREST NEIGHBOR
Authors: Azmi Azmi , Melliana Melliana , Mahmud Soni Fajar , Rahman Hasbur 
Published: February 2025

UKM Mie Basah Sukiyo, produsen mie basah rumahan di Jl. Siak, Kota Dumai, Riau, telah beroperasi selama 25 tahun dan melayani beragam pedagang bakso di wilayah Kota Dumai. Saat ini, UKM ini menghadapi tantangan dalam pendistribusian produk, karena proses pengiriman belum berjalan secara efisien. Rute pengantaran belum dirancang secara optimal dan cenderung memprioritaskan pelanggan dengan permintaan tertinggi tanpa memperhitungkan efektivitas rute keseluruhan. Penelitian ini bertujuan untuk merancang rute distribusi yang lebih efisien dan terstruktur sekaligus meminimalkan biaya distribusi. Metode yang digunakan dalam penelitian ini adalah Saving Matrix dan Nearest Neighbor. Analisis menggunakan Saving Matrix berhasil mengurangi jumlah rute dari empat menjadi dua rute, sementara optimasi urutan pengiriman dengan Nearest Neighbor menurunkan jarak tempuh dari 43,1 km menjadi 39,3 km. Pengurangan jarak ini secara langsung menurunkan biaya transportasi bulanan dari Rp323.250 menjadi Rp293.750, sehingga terjadi penghematan sebesar Rp28.500 atau 8,81%.Kata kunci: Distribusi, Nearest Neighbour, Saving Matrix.
ALGORITMA AES 128 DALAM MENGENKRIPSIKAN BERKAS BANSOS PADA KECAMATAN TIGABINANGA BERBASIS WEB
Authors: Tarigan Yusril Agita Prayoga , Aulia Rachmat , Elhanafi Andi Marwan 
Published: February 2025

Kecamatan Tiga Binanga, yang terletak di Kabupaten Karo, Provinsi Sumatera Utara. Dengan luas wilayah mencapai 160,38 km², jumlah penduduk yang tercatat sebesar 20.346 jiwa pada 24 Februari 2021, kecamatan ini memiliki kepadatan penduduk 127 jiwa/km², menjadikannya salah satu kecamatan yang cukup padat di Kabupaten Karo.
Kegiatan pembagian bantuan sosial (bansos) di Kecamatan Tiga Binanga dilakukan secara rutin untuk mendukung masyarakat. Namun, seiring dengan perkembangan teknologi, data digital seringkali menghadapi risiko keamanan, termasuk kemungkinan pengubahan atau akses tidak sah oleh pihak yang tidak bertanggung jawab. Untuk mengatasi masalah ini, diperlukan metode yang efektif untuk melindungi data. Salah satu solusi yang dapat diterapkan adalah teknik kriptografi. Penelitian ini mengusulkan penggunaan Algoritma AES (Advanced Encryption Standard) 128 untuk mengenkripsi file bansos. Aplikasi berbasis web ini dikembangkan menggunakan bahasa pemrograman PHP untuk memastikan keamanan data. Dengan penerapan Algoritma AES 128, diharapkan aplikasi ini dapat menjaga kerahasiaan dan integritas data bansos, mencegah kebocoran informasi, dan melindungi data dari pencurian. Proses enkripsi yang diterapkan akan menghasilkan output yang tidak dapat diakses atau dibaca oleh pihak yang tidak berwenang.

Kata kunci: Bansos; Algoritma AES 128; PHP
Investigating the role of pharmacogenomics in optimizing pediatric medication therapy: a pilot study
Authors: Ranjan Dash Smruti , Kumar Sahu Mahendra 
Published: February 2025

The Food and Medication Organization and the Public Establishments of Wellbeing have worked to move pharmacogenomics from the laboratory to the patient's bedside. Pharmacogenomic data is now included on the label of more than 100 pharmaceutical drugs. In the pediatric population, several of these are often utilized. Additionally, medical practitioners must intervene and provide advice regarding direct-to-consumer genetic test kits. Healthcare workers must gain a basic understanding of pharmacogenomics and how it applies to patient care in light of the growing trend toward customized treatment. Because pharmacogenomic testing can provide patient-explicit indicators for medication reaction and security, pharmacologists can effectively participate in clinical understanding of results, pharmacogenomic testing, and ideas for individualizing drug treatment. In both short-term and long-term contexts, drug specialists can identify possible opportunities, such as demonstrating pharmacogenomic testing to patients and their families and supervising the implementation of clinical pharmacogenomics meetings. Pharmacists may work in clinical settings as well as in medication development and discovery that is impacted by genetics. The creation of interprofessional practice guidelines and dosing recommendations for pharmacogenomic testing in pediatric patients should involve pediatric pharmacists because of the possibility that age-dependent and genetic factors would affect drug selection and dosage. The purpose of this pilot project is to investigate how pharmacogenomics can be used to customize prescription regimens for young patients in order to increase effectiveness and lower adverse drug reactions (ADRs).