bluuebunny/crossref_metadata_2025_split huggingface dataset:
Atheroma-relevant 7-oxysterols differentially upregulate CD14 expression
Authors: Kim Bo-Young , Son Yonghae , Kim Byoung Joon , Chung Sung-Woon , Lee Dongjun , Eo Seong-Kug , Kim Koanhoi 
Published: January 2023

Abstract
The expression of CD14 in monocytes/macrophages is elevated in atherosclerotic lesions where 7-oxyterols are abundant. However, it remains unknown whether atheroma-relevant 7-oxysterols are involved in receptor expression. Therefore, we investigated the effects of 7α-hydroxycholesterol (7αOHChol), 7β-hydroxycholesterol (7βOHChol), and 7-ketocholesterol (7K) on CD14 levels in THP-1 monocytes/macrophages. The three 7-oxysterols increased CD14 transcript levels at a distinct time point, elevated cellular CD14 protein levels, and promoted the release of soluble CD (sCD14) from THP-1 cells. Our data revealed that CD14 expression was most strongly induced after treatment with 7αOHChol. However, they did not affect CD14 transcript levels in vascular smooth muscle cells and Jurkat T cells. Moreover, 7αOHChol alone upregulated membrane-bound CD14 levels and enhanced responses to lipopolysaccharides, as determined by CCL2 production and monocytic cell migration. The 7-oxysterols also increased the gelatinolytic activity of MMP-9, and a cell-permeable, reversible MMP-9 inhibitor, MMP-9 inhibitor I, significantly impaired sCD14 release. These results indicate that 7-oxysterols differentially induce CD14 expression in vascular cells and contribute to the monocyte/macrophage expression of CD14 via overlapping but distinct mechanisms.
Effect of Meteorological Factors, Air Pollutants on Daily Hospital Admissions for Ischemic Heart Disease in Lanzhou, China
Authors: Meng Xiaoxue , Jin Jianjian , Han Xia , Han Bing , Bai Ming , Zhang Zheng 
Published: January 2023

Abstract
Background: Meteorological factors and air pollutants are believed to be associated with cardiovascular disease. Ischemic heart disease (IHD) is a major public health issue worldwide. Few studies have investigated the associations among meteorological factors, air pollutants and IHD daily hospital admissions in Lanzhou, China.
Methods: We conducted a distributed lag non-linear model (DLNM) on the basis of five years data, aiming at disentangling the impact of meteorological factors and air pollutants on IHD hospital admissions. All IHD daily hospital admissions recorded from January 1, 2015 and December 31, 2019 were obtained from three hospitals in Lanzhou, China. Daily air pollutant concentrations and meteorological data were synchronously collected from Gansu Meteorological Administration and Lanzhou Environmental Protection Administration. Stratified analyses were performed by sex and two age-groups.
Results: A total of 23555 IHD hospital admissions were recorded, of which 10477 admissions were for coronary artery disease (CAD), 13078 admissions were for acute coronary syndrome (ACS). Our results showed that there was a non-linear (J-shaped) relationship between temperature and IHD hospital admissions. The number of IHD hospital admissions were positively correlated with NO2, O3, humidity and pressure, indicating an increased risk of hospital admissions for IHD under NO2, O3, humidity and pressure exposure. Meanwhile, both extremely low (-12ºC) and high (30ºC) temperature reduced IHD hospital admissions, but the harmful effect increased with the lag time in Lanzhou, China, while the cold effect was more pronounced and long-lasting than the heat effect. Subgroup analysis demonstrated that the risk on CAD hospital admissions increased significantly in female and <65 years of age at -12ºC.
Conclusion: Our findings added to the growing evidence regarding the potential impact of meteorological factors, air pollutants on policymaking from the perspective of hospital management efficiency.
Clinical outcomes of bilateral implantation of new generation monofocal IOL enhanced for intermediate distance and conventional monofocal IOL in a Korean population
Authors: Choi Wan Kyu , Han Hyo Ji , Son Hyeck Soo , Khoramnia Ramin , Auffarth Gerd Uwe , Choi Chul Young 
Published: January 2023

Abstract
Background:
To compare the clinical outcomes of bilateral implantation of enhanced intermediate function intraocular lenses (IOLs) and standard monofocal IOLs.
Methods:
In this prospective, randomized, comparative controlled study, we compared the visual outcomes of patients who underwent bilateral cataract surgery at the Kangbuk Samsung Hospital, Sungkyunkwan University School of Medicine, with either enhanced monofocal IOLs (Tecnis Eyhance, ICB00, Johnson and Johnson Vision Care, Inc) (Group 1) or standard monofocal IOLs (Tecnis, ZCB00, Johnson and Johnson Vision Care, Inc) (Group 2). The assessment included monocular and binocular uncorrected distance visual acuity (UDVA), uncorrected intermediate (UIVA at 60 cm) and near (UNVA at 40 cm) visual acuity, uncorrected defocus curves, contrast sensitivity testing (CST), and reading speed test using Quality of vision was evaluated using the Visual Function Questionnaire (VFQ-25).
Results:
At 3-months postoperatively, monocular and binocular outcomes of UIVA and UNVA were statistically significantly better in Group 1 (P<0.05). The binocular uncorrected defocus curve of Group 1 showed statistically significantly better outcomes at vergence ranges of -1.5 to -4.0 D (P<0.05). Significantly higher reading speed test was also observed in Group 1 in all ranges tested (1.0 to 0.1 LogMAR) (P<0.05). There were no statistically significant differences in CST between groups.
Conclusions:
Bilateral implantation of enhanced monofocal IOLs provided better vision at intermediate and near distances compared to standard monofocal IOLs, while maintaining good distance vision and contrast sensitivity.
Gene orthology inference via large-scale rearrangements for partially assembled genomes
Authors: Rubert Diego P. , Braga Marilia D. V. 
Published: January 2023

Abstract
Background: Recently we developed a gene orthology inference tool based on genome rearrangements (Journal of Bioinformatics and Computational Biology 19:6, 2021). Given a set of genomes our method first computes all pairwise gene similarities. Then it runs pairwise ILP comparisons to compute optimal gene matchings, which minimize, by taking the similarities into account, the weighted rearrangement distance between the analyzed genomes (a problem that is NP-hard). The gene matchings are then integrated into gene families in the final step. Although the ILP is quite efficient and could conceptually analyze genomes that are not completely assembled but split in several contigs, our tool failed in completing that task. The main reason is that each ILP pairwise comparison includes an optimal capping that connects each end of a linear segment of one genome to an end of a linear segment in the other genome, producing an exponential increase of the search space.
Results: In this work, we design and implement a heuristic capping algorithm that replaces the optimal capping by clustering (based on their gene content intersections) the linear segments into m ≥ 1 subsets, whose ends are capped independently. Furthermore, in each subset, instead of allowing all possible connections, we let only the ends of content-related segments be connected. Although there is no guarantee that m is much bigger than one, and with the possible side effect of resulting in sub-optimal instead of optimal gene matchings, the heuristic works very well in practice, from both the speed performance and the quality of computed solutions. Our experiments on fruit fly genomes show two positive results. First, for complete assemblies the version with heuristic capping reports orthologies that are very similar to the orthologies computed by the version of our tool with optimal capping. Second, we were able to efficiently analyze genomes with incomplete assemblies distributed in hundreds or even thousands of contigs, obtaining orthologies that are more similar to FlyBase orthologies when compared to orthologies computed by other inference tools. We added a post-processing for refining, with the aid of the mcl algorithm, our ambiguous families (those with more than one gene per genome), improving even more the accuracy of our results. Our approach is implemented into a pipeline incorporating the pre-computation of gene similarities and the post-processing refinement of ambiguous families with mcl. We optimized several aspects of our implementation, achieving running times that are similar to the fastest alternative tools. Both the original version with optimal capping and the new modified version with heuristic capping can be downloaded from our GitLab server at gitlab.ub.uni-bielefeld.de/gi/FFGC.
IDENTIFICATION OF HUMAN GENETIC VARIANTS MODULATING THE COURSE OF COVID-19 INFECTION
Authors: Ašić Adna , Salihefendić Lana , Čeko Ivana , Bešić Larisa , Mulahuseinović Naida , Durgut Selma , Pećar Dino , Prnjavorac Lejla , Kandić Enis , Meseldžić Neven , Bego Tamer , Prnjavorac Besim , Marjanovic Damir , Konjhodžić Rijad 
Published: January 2023

Abstract
COVID-19, caused by the SARS-CoV-2 virus, has been a major focus of scientific research since late 2019 and early 2020. Due to its enormous societal, economic, and clinical impact worldwide, research efforts aimed, among other questions, to address the effect of host genetics in susceptibility and severity of COVID-19. In this research, we performed next-generation sequencing of coding and regulatory regions of 16 selected human genes, involved in the maintenance of the immune system or encoding the receptors for viral entry into the host cells, in a subset of 60 COVID-19 patients from the General Hospital Tešanj, Bosnia and Herzegovina, classified into three groups of patients with clinical conditions of different severity (“mild”, “moderate”, and “severe” clinical groups). In accordance with previous studies, we found out that the male sex and older age are risk factors for severe clinical picture. We identified 13 variants on seven genes (CD55, IL1B, IL4, IRF7, DDX58, TMPRSS2, and ACE2) with potential functional significance, either as genetic markers of modulated susceptibility to SARS-CoV-2 infection or as modifiers of the course of infection in terms of predicted symptom severity. Our results include variants reported for the first time as potentially associated with COVID-19. Future studies on larger patient cohorts, focused on candidate genes and/or candidate genetic variants, have a potential to answer a range of open questions regarding the effect of host (human) genetic makeup on the expected outcome of COVID-19.
Source Reconstruction of the ERP Uncertainty Effects reveals common Neural Mechanisms for different Stimulus Categories
Authors: Hecker Lukas , Joos Ellen , Feige Bernd , Maier Simon , Elst Ludger Tebartz van , Kornmeier Jürgen 
Published: January 2023

Abstract
During the observation of an ambiguous figure, our perception becomes unstable and alternates repeatedly between mutual exclusive interpretations. Tiny changes of the stimulus features can disambiguate the figure and stabilize percepts. Recent EEG studies found much smaller amplitudes of two event-related potentials (ERPs), an anterior P200, 200 ms after stimulus onset and a posterior P400, 200 ms later, when participants observed an ambiguous stimulus compared to disambiguated stimulus variants. Interestingly, this pattern of results was found across stimuli differing in ambiguity (geometry, motion and gestalt) and in visibility. We postulate a meta-perceptual / meta-cognitive evaluation instance that rates the reliability of perceptual constructs at a high processing level generalized beyond sensory details. We further postulate that the above described ERP effects reflect the outcome of this evaluation process.
According to these hypotheses, the distributions of these ERP effects on the scalp across three different stimulus categories, should originate from the same neural structures in the sensor and source space. This was tested by calculating EEG inverse solutions using a novel artificial neural network-based approach.We found very similar sources across stimulus categories, both on the level of individual participants and on the group level. Regions involved in the earlier processing steps (P200) encompass lateral occipital cortex, inferior parietal cortex and medial cingulate cortex. Later processes (P400) originated mostly from the right inferior temporal cortex. Our findings were consistent with comparable studies using functional magnetic resonance imaging and EEG source imaging. In summary, we found highly coherent neural sources of the ERP Uncertainty Effects. The underlying processes may be related to a common uncertainty resolution at a higher processing level beyond sensory details.
Refractory Atrial Flutter, the Only Presentation of covid-19 Infection in a Neonate: a case report and review of literature
Authors: Saadat Seyed Hossein , Tariverdi Marjan , jahromi mohammadreza kargarfard , sharma Deepak , Farahbakhsh Nazanin 
Published: January 2023

Abstract
Background

The accurate incidence of different cardiovascular consequences of COVID-19 in pediatric population have been inadequately defined due to ongoing genotype changes in the virus. Although COVID-19 is known to increase inflammatory markers associated with atrial arrhythmias, the contemporary literature has poorly described new onset arrhythmias as a complication in previously healthy neonate with COVID-19

Case presentation

Twenty-day-old female term neonate, born by caesarean section with immediate cry developed labored breathing, cyanosis and tachycardia after having close contact with a confirmed case of covid-19. She developed atrial flutter, which was refractory to cardioversion and drugs namely Amiodarone, Flecainide and Propranolol. The neonate was treated with IVIG. This is the first reported case of atrial flutter in neonatal period secondary to COVID-19.

Conclusion

Since the starting of SARS –COV2 pandemic all attentions and concerns has been mainly on respiratory manifestations and complications. The cardiovascular complications and treatment are neglected. This case reports tachyarrhythmia (Atrial Flutter) as unusual presentation of MISC in neonatal population and show role of IvIg in treatment of refractory arrhythmias.
Plasmepsin X activates function of the PCRCR complex in P. falciparum by processing PfRh5 for binding to basigin and invasion of human erythrocytes
Authors: Triglia Tony , Scally Stephen , Seager Benjamin , Pasternak Michal , Dagley Laura , Cowman Alan 
Published: January 2023

Abstract
Plasmodium falciparum causes the most severe form of malaria in humans. The protozoan parasite develops within erythrocytes to mature schizonts, that contain more than 16 merozoites, which egress and invade fresh erythrocytes. The aspartic protease plasmepsin X (PMX), processes proteins and proteases essential for merozoite egress from the schizont and invasion of the host erythrocyte, including the leading vaccine candidate PfRh5. PfRh5 is anchored to the merozoite surface through a 5-membered complex (PCRCR), consisting of Plasmodium thrombospondin-related apical merozoite protein (PTRAMP), cysteine-rich small secreted protein (CSS), Rh5-interacting protein (PfRipr) and cysteine-rich protective antigen (CyRPA). We show that PCRCR is processed by PMX in micronemes to remove the N-terminal prodomain of PhRh5 and this activates the function of the complex unmasking a form that can bind basigin on the erythrocyte membrane and mediate merozoite invasion. The ability to activate PCRCR at a specific time in merozoite invasion most likely masks potential deleterious effects of its function until they are required. These results provide an important understanding of the essential role of PMX and the fine regulation of PCRCR function in P. falciparum biology.
Comparison of target antigen and immunoglobulin isotypes in anti-SARS-CoV-2 antibodies from natural infection and vaccination
Authors: Kim Jeong Hui , Kikuchi Ryosuke , Suzuki Atsuo , Watarai Rika , Goto Kaori , Okumura Yui , Matsushita Tadashi 
Published: January 2023

Abstract
The immune system produces antibodies following SARS-CoV-2 infection and vaccination. However, we lack comprehensive information about the humoral responses after infection and vaccination, which are similar but might differ in type or amount of produced antibodies. Therefore, we compared different types and amounts of antibodies produced by the immune system in response to infection versus those produced by BNT162b2 (Pfizer/BioNTech) vaccines. Our findings indicated that naturally infected individuals had 14-fold higher anti-SARS-CoV-2 spike protein (anti-S) and 41-fold higher anti-SARS-CoV-2 receptor-binding domain of the viral spike protein (anti-RBD) IgM titers than SARS-CoV-2-naïve vaccinees. A significantly rapid increase in anti-S IgG titers in primary infection was observed, eventually reaching a level similar to that in people who had third booster vaccination. Moreover, a significant positive correlation was observed between anti-S and anti-RBD levels in naturally infected and BNT162b2-vaccinated individuals. Collectively, our data suggest that anti-RBD and anti-S IgG can serve as neutralizing antibody predictors during routine laboratory testing.
Prospecting the Intricate Role of Intestinal Microbe and Microbial Metabolite Biomarkers in Schizophrenia
Authors: Shi Li , Ju Peijun , Meng Xiaojing , Wang Zhongxian , Yao Lihui , Zheng Mingming , Cheng Xialong , Li Jingwei , Yu Tao , Xia Qingrong , Yan Junwei , Zhu Cuizhen , Zhang Xulai 
Published: January 2023

Abstract
Background: The brain-gut axis has gained increasing attention due to its contribution to the etiology of various central nervous system disorders. In particular, impaired intestinal microflora and intestinal metabolite dysbiosis have been implicated in schizophrenia.
Methods: In this study, we used a 16S ribosomal RNA (16S rRNA) gene sequence–based approach and an untargeted liquid chromatography-mass spectrometry-based metabolic profiling approach to measure the gut microbiome and microbial metabolites from 44 healthy controls, 41 acute patients, and 39 remission patients, to evaluate whether microbial dysbiosis and microbial metabolite biomarkers were linked with the severity of schizophrenic symptoms.
Results: Here, we identified 20 dominant disturbances in the gut microbial composition of patients compared with healthy controls, with 3 orders, 4 families, 9 genera, and 4 species. Several unique bacterial taxa associated with schizophreniaseverity. Compared with healthy controls, 145 unusual microflora metabolites were detected in the acute and remission groups, which were mainly involved in environmental information processing, metabolism, organismal systems, and human diseases in the Kyoto encyclopedia of genes and genomes pathway. The Sankey diagram showed that 4 abnormal intestinal and 4 anomalous intestinal microbial metabolites were associated with psychiatric clinical symptoms.
Conclusions: These findings suggest a possible interactive influence of the gut microbiota and their metabolites on the pathophysiology of schizophrenia.
Comparison of key genes regulating the prognosis of HBV/HCV-related hepatocellular carcinoma
Authors: Huang Yiwen , Wang Shangzi , Yuan Huangbo , Liu Zhenqiu , Zhang Tiejun 
Published: January 2023

Abstract
Background Hepatocellular carcinoma (HCC) is one of the most common cancers worldwide. Despite the decrease in both incidence and mortality rates in the last few years, Hepatitis B virus (HBV) and Hepatitis C virus (HCV) infection account for 70% of global liver cancer deaths. However, few researches have explored the prognostic similarities and differences between HBV-related HCC (HBV-HCC) and HCV-related HCC (HCV-HCC) at transcript level. Therefore, we aim to identify genes that may play an important role in regulating prognosis for HBV-HCC and HCV-HCC respectively and potentially lead to the prognostic difference between HBV-HCC and HCV-HCC.
Methods Weighted correlation network analysis (WGCNA) was first conducted to identify gene modules related with prognosis using The Cancer Genome Atlas Liver Hepatocellular Carcinoma (TCGA-LIHC) cohort. The K-means clustering algorithm, Univariate and Multivariate Cox regression were, in turn, used to identify hub gene related to prognosis. The hub genes were then validated using ICGC-LIRI-JP cohort from The International Cancer Genome Consortium(ICGC) database by Multivariate Cox regression. Lastly, the validated genes in each group were alternately validated in other group.
Results Midnightblue and paleturquoise modules in HBV group, greenyellow and white modules in HCV group were selected during WGCNA. Later, CALCRL, TMEM204, APLNR, MED8, HSPB11, TRAPPC3, ADPRS and BTF3L4 in HBV group, NEU1, DHX16, EDEM3, GLRX2 and CYB5R1 in HCV group were screened as our hub genes. Eventually, during validation phase, TMEM204 remained significant in both groups, and CYB5R1 was only significant in HCV group.
Conclusions Our study suggested that there may be substantial prognostic differences between HBV-HCC and HCV-HCC in terms of mechanism. CYB5R1 may be partially related to prognostic difference between HBV-HCC and HCV-HCC.
Grafted Amberlite 200 C resin for adsorptive removal of salicylic acid
Authors: SAOUD Fairouz SAAD , BERBAR Yassine , AMARA Mourad 
Published: January 2023

Abstract
Removal of salicylic acid (SA) present in the pharmaceutical aqueous waste was performed by unmodified and modified commercial cationic exchange resin (Amberlite 200C). Modification of commercial resin was carried out by grafting of functional molecules either into aromatic ring using classical organic reaction or by physical irreversible adsorption in the resin skeleton. Nitro group NO2+ was prepared by mixing nitric and sulfuric acids and fixed by nitration whereas 2,4-dinitrophenyl hydrazine (DNPH), tributyl phosphate (TBP), and NH4+ were adsorbed on the resin matrix by immersion. As a result, unmodified resin allow elimination 21% of initial salicylic acid. While the yield of salicylic acid elimination was found to be notably increased by using modified resins. Elimination of SA by NH4+ and DNPH modified resins reached respectively 51.94 and 41.5%. In the same purpose, it has been observed no consequently removal of SA when TBP and NO2+ modified resins were employed. It was noted that the optimal operational conditions were pH=5.5 T° = 21°C, t = 20 min
Exome sequencing reveals that the CYP1B1 and FOXC1 genes are mainly responsible for childhood glaucoma in Japanese patients
Authors: Fuse Nobuo , Kimura Masae , Shimizu Ai , Hamanaka Teruhiko , Nakamura Makoto , Ishida Nobuo , Sakai Hiroshi , Ikeda Yoko , Mori Kazuhiko , Endo Atsushi , nagasaki Masao , Katsuoka Fumiki , Yasuda Jun , Matsubara Yoichi , Nakazawa Toru , Yamamoto Masayuki 
Published: January 2023

Abstract
Childhood glaucoma is a group of heterogeneous genetic disorders. The purpose of this study was to explore the genetic background in the Japanese population. Genomic DNA was extracted from 31 patients with childhood glaucoma from 29 families in the Japanese population. We screened the CYP1B1, FOXC1 and candidate genes using Sanger sequencing and whole-exome sequencing (WES). In the CYP1B1 gene, we identified nine mutations, of which four were novel. Almost all affected individuals had severe early-onset childhood glaucoma. In the FOXC1 gene, three novel mutations were identified in a heterozygous state. We next attempted to extract the candidate genes from the subjects showing negative results for two genes. The iterative filtering process by WES revealed 4 single-nucleotide variations (SNVs) in the PTPRF, SMPD4, VPS13B, and DHRS1 genes on autosomal chromosomes and 4 SNVs in the NHS, KCND1, BRWD3, and ENOX2 genes on the X chromosome. The CYP1B1 and FOXC1 genes are major causal genes of childhood glaucoma in Japanese families (30% and 10%, respectively), and WES results reveal the heterogeneity of the genetic background. Screening the CYP1B1 and FOXC1 genes is useful to ensure the proper diagnosis and adequate treatment of childhood glaucoma.