bluuebunny/crossref_metadata_2025_split huggingface dataset:
Niche differential gene expression analysis in spatial transcriptomics data identifies context-dependent cell-cell interactions
Authors: Mason Kaishu , Sathe Anuja , Hess Paul , Rong Jiazhen , Wu Chi-Yun , Furth Emma , Ji Hanlee P. , Zhang Nancy 
Published: January 2023

AbstractSingle cells influence, and are shaped by, their local spatial niche. Technologies for in situ measurement of gene expression at the transcriptome scale have enabled the detailed profiling of the spatial distributions of cell types in tissue as well as the interrogation of local signaling patterns between cell types [1]. Towards these goals, we propose a new statistical procedure called niche-differential expression (niche-DE) analysis. Niche-DE identifies cell-type specific niche-associated genes, defined as genes whose expression within a specific cell type is significantly up- or down-regulated, in the context of specific spatial niches. We develop effective and interpretable measures for global false discovery control and show, through the analysis of data sets generated by myriad protocols, that the method is robust to technical issues such as over-dispersion and spot swapping. Niche-DE can be applied to low-resolution spot- and ROI-based spatial transcriptomics data as well as data that is single-cell or subcellular in resolution. Based on niche-DE, we also develop a procedure to reveal the ligand-receptor signaling mechanisms that underlie niche-differential gene expression patterns. When applied to 10x Visium data from liver metastases of colorectal cancer, niche-DE identifies marker genes for cancer-associated fibroblasts and macrophages and elucidates ligand-receptor crosstalk patterns between tumor cells, macrophages and fibroblasts. Co-detection by indexing (CODEX) was performed on the same patient samples, to corroborate the niche-DE results.
Identification of a new antiphage system inMycobacteriumphage Butters
Authors: Mohammed Hamidu T. , Mageeney Catherine , Ware Vassie C. 
Published: January 2023

ABSTRACTDuring lysogeny temperate phages establish a truce with the bacterial host. In this state, the phage genome (prophage) is maintained within the host environment. Consequently, many prophages have evolved systems to protect the host from heterotypic viral attack. This phenomenon of prophages mediating defense of their host against competitor phages is widespread among temperate mycobacteriophages. We previously showed that theMycobacteriumphage Butters prophage encodes a two-component system (gp30/31) that inhibits infection from a subset of mycobacteriophages that include PurpleHaze, but not Island3. Here we show that Butters gp57r is both necessary and sufficient to inhibit infection by Island3 and other phages. Gp57r acts post-DNA injection and its antagonism results in the impairment of Island3 DNA amplification. Gp57r inhibition of Island3 is absolute with no defense escape mutants. However, mutations mapping to minor tail proteins allow PurpleHaze to overcome gp57r defense. Gp57r has a HEPN domain which is present in many proteins involved in inter-genomic conflicts, suggesting that gp57r may inhibit heterotypic phage infections via its HEPN domain. We also show that Butters gp57r has orthologues in clinical isolates ofMycobacterium abscessus sp. including the phage therapy candidate strain GD91 which was found to be resistant to the panel of phages tested. It is conceivable that this GD91 orthologue of gp57r may mediate resistance to the subset of phages tested. Challenges of this nature underscore the importance of elucidating mechanisms of antiphage systems and mutations that allow for escape from inhibition.IMPORTANCEThe evolutionary arms race between phages and their bacteria host is ancient. During lysogeny, temperate phages establish a ceasefire with the host where they do not kill the host but derive shelter from it. Within the phenomenon of prophage-mediated defense, some temperate phages contribute genes that make their host more fit and resistant to infections by other phages. This arrangement has significance for both phage and bacterial evolutionary dynamics. Further, the prevalence of such antiphage systems poses a challenge to phage therapy. Thus, studies aimed at elucidating antiphage systems will further our understanding of phage-bacteria evolution as well as help with efforts to engineer therapeutic phages that circumvent antiphage systems.
The interfascicular matrix of energy storing tendons houses heterogenous cell populations disproportionately affected by ageing
Authors: Zamboulis Danae E. , Marr Neil , Lenzi Luca , Birch Helen L. , Screen Hazel R. C. , Clegg Peter D. , Thorpe Chavaunne T. 
Published: January 2023

AbstractEnergy storing tendons such as the human Achilles and equine superficial digital flexor tendon (SDFT) are prone to injury, with incidence increasing with ageing. The interfascicular matrix (IFM), which binds tendon fascicles, plays a key role in energy storing tendon mechanics, and ageing alterations to the IFM negatively impact tendon function. While the mechanical role of the IFM in tendon function is well-established, the biological role of IFM-resident cell populations remains to be elucidated. Therefore, the aim of this study was to identify IFM-resident cell populations and establish how these populations are affected by ageing. Cells from young and old SDFTs were subjected to single cell RNA-sequencing, and immunolabelling for markers of each resulting population used to localise cell clusters. Eleven cell clusters were identified, including tenocytes, endothelial cells, mural cells and immune cells. One tenocyte cluster localised to the fascicular matrix, whereas nine clusters localised to the IFM. Interfascicular tenocytes and mural cells were preferentially affected by ageing, with differential expression of genes related to senescence, dysregulated proteostasis and inflammation. This is the first study to uncover the importance of the IFM niche for a diverse range of cell populations, and to identify age-related alterations specific to IFM-localised cells.
The supports provided by artificial intelligence to continuous usage intention of mobile banking: evidence from China
Authors: Lin Rong-Rong , Lee Jung-Chieh 
Published: January 2023

PurposeArtificial intelligence (AI) has been widely used as a financial technology (fintech) in the mobile banking (M-banking) domain. However, in the literature, how AI affects users' perceptions of social support and the users' satisfaction and continuance intention (CI) remains unknown. To fill this gap, the two core characteristics of AI, perceived intelligence (PI) and perceived anthropomorphism (PA), are combined with social support theory (SST) (including informational support (IS) and emotional support (ES)) to develop a research model to investigate how PI and PA affect IS and ES, which in turn affect users’ M-banking satisfaction and CI.Design/methodology/approachThis study adopted a random probability sampling method to collect a total of 360 valid responses to verify the proposed model. Partial least squares (PLS) was employed for data analysis.FindingsThe results showed that PI and PA both have a significant positive impact on consumers' perception of social support (IS and ES). IS was a direct driver of satisfaction and CI. Surprisingly, although ES was positively associated with satisfaction, the study found that higher levels of ES will decrease CI. This study exposed how AI affects consumers’ satisfaction and CI through SST, and the role of AI in M-banking applications has been further confirmed.Originality/valueThis study expanded the SST to creatively integrate with AI features to reveal the impact of PI and PA on IS and ES, which in turn influence users' M-banking usage.
Influence of friction stir welding parameters on the tribological behavior of dissimilar aluminum alloy joint
Authors: S. Sivaselvan , M. Natarajan , S.R. Devadasan , N.M. Sivaram 
Published: January 2023

PurposeAluminum alloys are applicable in marine and aero fields. Alloys AA5083 and AA6061 are aluminum alloys with different chemical and physical properties. Combination of two dissimilar materials could result in enhanced strength. Generally, dissimilar aluminum alloy joint is made by friction stir welding (FSW) to achieve improved physical properties compared with the parent alloys. The purpose of this research is to develop a new FSW dissimilar material with enhanced properties using AA5083 and AA6061 alloys.Design/methodology/approachIn this research, FSW joint was made for butt joint configuration using AA5083 and AA6061 aluminum alloys. Cylindrical pin with threaded profile was used to perform the joint. The tool tilting angle was maintained as constant, and the tool rotational speed and the welding speed were varied. Wear performance and mechanical strength of the joint were analyzed.FindingsThe results revealed that the increase of tool rotational speed led to poor wear performance, whereas increase of welding speed showed a better wear performance. Further, the prepared joint was analyzed for different wear parameters such as sliding velocity and applied load. The results displayed that the increase of sliding velocity exhibited low wear rate and the increase of load showed high wear rate.Originality/valueThis work is original and deals with the wear performance of AA5083–AA6061 joint at different tool rotational and welding speeds.
How to earn a premium price: the effect of green marketing and brand coolness
Authors: Guerreiro João , Loureiro Sandra Maria Correia , Nascimento Jorge , Duarte Miguel 
Published: January 2023

PurposeThe current paper aims to explore how brand coolness can mediate the relationship between tactical green marketing orientation (GMO) and willingness to pay (WTP), by exploring the differences between two global brands with opposite green marketing perceptions.Design/methodology/approachBased on the stimuli-organism-response (S-O-R) framework, the relation between tactical GMO, brand coolness and consumer's WTP is examined through a survey with 272 participants, who gave their perceptions about two different brands regarding their green orientations: British Petroleum (BP) and L'Oréal. The variable set was adapted and validated through focus group sessions.FindingsBrand coolness is found to mediate the impact GMO on WTP and, for both brands, green marketing does affect the extent to which brands are perceived as cool by consumers. More importantly, evidence shows that only in the case of the “green brand” (e.g. L'Oréal), the impact on WTP is significant, which offers new implications regarding the outcomes of companies' pro-environmental policies.Originality/valueThis study is the first to investigate the outcomes of GMO over consumer's intentions (WTP) and the role of brand perceptions (coolness). The effects are compared between two global brands, with significantly different perceptions on their environmental sustainability.
An examination of the insulation of global worker communities for political reasons: the case of the J-1 Visa in the United States
Authors: Ryan Jason , Silvanto Sari 
Published: January 2023

PurposeThe insulation of globally mobile workers into communities is often influenced by political considerations within the host country. Most studies examining this area have focused on how expatriates and globally mobile workers seclude themselves into insulated communities when working abroad. This perspective does not take into consideration political and societal factors that often pressure globally mobile workers into secluded communities composed of people similar to themselves. This study examines how host-country political imperatives can help create and maintain insulated communities of foreign workers.Design/methodology/approachThis study is based on a case study methodology that examines the J-1 Visa program in the United States and how it has, in many instances, evolved into a de facto guest worker program that secludes foreign workers into insulated communities. The case study includes interviews with five J-1 administrators at sponsoring organizations that employ J-1 recipients.FindingsThis study finds that political pressures do have an impact on the insulation of J-1 recipients into secluded communities in the United States. This is largely accomplished through the legal requirements of the program, pressure from sponsoring employers on the government and the significant political and economic ties that the United States maintains with the home countries of J-1 recipients.Originality/valueThis is one of the few studies to examine insulated communities of globally mobile workers from a political perspective. It is exploratory in nature and recommends that further studies be conducted.
Stock market integration and volatility spillovers: new evidence from Asia–Pacific and European markets
Authors: Guru Biplab Kumar , Yadav Inder Sekhar 
Published: January 2023

PurposeThis work investigates the volatility spillovers across stock markets and the nature of such spillovers through different periods of crises and tranquility.Design/methodology/approachUsing daily stock return volatility data from June 2003 to June 2021, the generalized forecast error variance decomposition method (based on Diebold and Yilmaz, 2012 approach) is employed to measure the degree of volatility spillovers/connectedness among stock markets of 24 Asia–Pacific and 12 European Union (EU) economies.FindingsThe empirical results from static analysis suggested that about 28.1% (63.7%) of forecast error variance in return volatility for Asia–Pacific (EU) markets is due to spillovers. The evidence from dynamic analysis suggested that during mid of the global financial crisis, European debt crisis (EDC) and Covid-19, the gross volatility spillovers for Asia–Pacific (EU) was around 67% (80%), 65% (80%) and 73% (67%), respectively. The degree of net volatility transmission from Singapore (Denmark) to other Asia–Pacific (EU) markets was found to be highest.Practical implicationsThe findings have crucial implications for the investors and portfolio managers in assessment of risk and optimum allocation of assets and investment decisions.Originality/valueThis study adds to the literature on risk management by systematically examining the impact of global financial crises, EDC and Covid-19 on the market interactions by capturing the magnitude, duration and pattern of the shock-specific market volatilities for a large sample of Asian and European markets using recent and large data set.
The impact of short sale prospect on income smoothing
Authors: Sun Xu , Zhang Tianming 
Published: January 2023

Purpose
The purpose of this paper is to examine the impact of short sale prospect on future income smoothing.

Design/methodology/approach
This study examines how short sale prospect impacts future income smoothing. This study follows prior research and uses two measures of income smoothing. One is the correlation between the change in prediscretionary income and the change in discretionary accruals. The other is the variability of earnings relative to the variability of cash flows.

Findings
This study finds that short sale prospect has a negative impact on future income smoothing. This finding is robust to use different measures of short sale prospect and income smoothing and to subsample tests. Additional analysis reveals that short sale prospect, by curbing income smoothing, reduces future stock price crash risk.

Originality/value
To the best of the authors’ knowledge, this study is the first to examine the impact of short selling on firms’ subsequent smoothing of reported income. This study contributes to the earnings quality literature by demonstrating the governance role of short selling on future earnings smoothness.
Do stakeholders’ interaction and collaboration influence CSR practice within supplier premises in Bangladesh? A managerial perception study
Authors: Hossain Md Tareq Bin , Momin Mahmood Ahmed , Dellaportas Steven 
Published: January 2023

Purpose
This study aims to investigate the influence of collaborative stakeholder relationships (buyers, media, government and top management) on apparel suppliers’ corporate social responsibility (CSR) in Bangladesh.

Design/methodology/approach
“Face to face” and “drop off and collect” survey administration techniques were used to collect a total of 371 questionnaires from middle-level managerial executives of apparel suppliers in Bangladesh. The data were analysed using partial least square structural equation modelling combined with resampling and bootstrapping techniques.

Findings
The findings suggest that buyers, the media and top management have a direct and significant influence on the stakeholder network and, in turn, positively impact the CSR of apparel suppliers in Bangladesh. The media and buyer firms work together to provide a combined and salient influence on the top management of supplier firms in Bangladesh to shape CSR practices.

Practical implications
The extent of stakeholder influence varies according to the strength of the network and the mediation within interconnected relationships. Suppliers’ top management could use the study’s findings to improve CSR by focussing on the strongest path of interconnected stakeholders. The Bangladesh Government could take policy initiatives to address CSR-related concerns raised by interconnected stakeholders.

Originality/value
This study contributes to stakeholder and CSR literature by providing valuable insights into the empirical justification of interactive stakeholder influences on suppliers’ CSR.
Modelling of the effects of luminaire installation geometries and other factors on road illumination system photometric parameters and energy efficiency
Authors: Bhattacharya Sourin , Majumder Sanjib , Roy Subarna 
Published: January 2023

PurposeProperly planned road illumination systems are collectively a public wealth and the commissioning of such systems may require extensive planning, simulation and testing. The purpose of this simulative work is to offer a simple approach to facilitate luminance-based road lighting calculations that can be easier to comprehend and apply to practical designing problems when compared to complex multi-objective algorithms and other convoluted simulative techniques.Design/methodology/approachRoad illumination systems were photometrically simulated with a created model in a validated software platform for specified system design configurations involving high-pressure sodium (HPS) and light-emitting diode (LED) luminaires. Multiple regression analyses were conducted with the simulatively obtained data set to propound a linear model of estimating average luminance, overall uniformity of luminance and energy efficiency of lighting installations, and the simulatively obtained data set was used to explore luminaire power–road surface average luminance characteristics for common geometric design configurations involving HPS and LED luminaires, and four categories of road surfaces.FindingsThe six linear equations of the propounded linear model were found to be well-fitted with their corresponding observation sets. Moreover, it was found that the luminaire power–road surface average luminance characteristics were well-fitted with linear trendlines and the increment in road surface average luminance level per watt increment of luminaire power was marginally higher for LEDs.Originality/valueThis neoteric approach of estimating road surface luminance parameters and energy efficiency of lighting installations, and the compendia of luminaire power–road surface average luminance characteristics offer new insights that can prove to be very useful for practical purposes.
Playing it safe; risk‐induced trait responses increase survival in the face of predation
Authors: Aguiar Olivia , Sonnega Sam , DiNuzzo Eleanor R. , Sheriff Michael J. 
Published: January 2023

AbstractPredation risk effects are impacts on prey caused by predators that do not include consumption. These can include changes in prey behaviour, physiology, and morphology (i.e. risk‐induced trait responses), which can have consequences to individual fitness and population dynamics (i.e. non‐consumptive effects). While these risk‐induced trait responses (RITRs) can lower individual fitness as compared to prey not exposed to risk, they are assumed to increase fitness in the presence of predators. While much work has been built upon this assumption, most evidence occurs in consumptive experiments where the trait values of consumed prey are unknown. We have little evidence showing individuals with a greater magnitude of RITR have greater survival.Here, we tested the hypothesis that RITRs increase survival in the presence of predators, but come at a cost to growth. We tested this hypothesis usingNucella lapillusas prey andCarcinus maenasas a predator and including mussels as a basal resource in a two‐phase mesocosm experimental set‐up. In phase 1,Nucellawere placed into either a control or risk treatment (exposure to non‐lethalCarcinus) for 28 days and their behaviour and growth measured. In phase 2, a lethalCarcinuswas added to all mesocosms (non‐lethal crabs were removed), and survival was recorded for 15 days.At the treatment (group) level, we found thatNucellaexposed to predation risk in phase 1 had significantly greater risk aversion behaviour (summed score of risky vs. safe behaviour) and significantly lower growth. In phase 2, we found thatNucellaexposed to predation risk had greater survival.At the individual level (regardless of treatment), we found thatNucellawith greater risk aversion scores in phase 1 had significantly higher survival in phase 2 when exposed to a lethal predator, but this came at a cost to their growth.This study provides some of the first empirical evidence, at both the group and individual level, testing a long‐held assumption that predation risk‐induced behavioural responses increase survival in the face of direct predation, but that these responses come at a cost to the prey. These results add to our growing understanding of the benefits of RITRs to individual fitness and non‐consumptive effects generally.
The determinants of office cap rates: The international evidence
Authors: Białkowski Jędrzej , Titman Sheridan , Twite Garry 
Published: January 2023

AbstractWe examine commercial office cap rates in 89 large cities in 33 developed and developing countries in the 2000–2019 period. We find that cap rates decline throughout the world over this period, reflecting a corresponding decline in the real rate of interest. In the cross‐city analysis our most robust findings are that office cap rates are lower in wealthier cities, especially those that are either considered gateway cities or financial centers. In addition, cap rates tend to be higher in countries with lower credit ratings and higher inflation rates. We find that cap rates in suburban office markets are higher than in central business districts, and for a given metropolis, suburban cap rates are lower in suburbs with better public transport connections to the central business district. Finally, evidence from regressions with city fixed effects reveal that cap rates rise as the discount rate and vacancy rates increase and fall as cities get wealthier.