bluuebunny/crossref_metadata_2025_split huggingface dataset:
Pharmacological Evaluations of Select Herbal Extracts on TLR7/8-induced Cytokine and Chemokine Production in Macrophage-like Cells
Authors: Dagar Manisha , Priya Kamala , Dikshit Madhu , Kumar Ajay 
Published: July 2023

AbstractInflammation is an innate immune response triggered by harmful stimuli, such as pathogens, tissue injury, or toxins. The purpose is to eliminate the source of infection and initiate the healing process. However, an excessive acute inflammatory response can lead to severe and life-threatening complications, as seen during recent pandemics. In the context of viral infections, the activation of the TLR7/8 signaling pathway has been implicated in excessive cytokine secretion. In this study, we aimed to replicate the perturbed inflammatory environment resulting from the activation of the TLR7/8 specific agonists, imiquimod, and resiquimod. We utilized macrophage-like cells, as macrophages are the first responders during infections and secrete key pro-inflammatory cytokines (TNF-α, IL-6, and IL-1β) to recruit immune cells to the site of infection. Herbal medicines have been traditionally used for centuries to enhance respiratory immune function. In the present study, we employed a prophylactic approach, where macrophage-like THP1 cells, differentiated with PMA, were pre-treated with select herbal extracts/formulations prior to TLR7/8 activation in the presence of agonists. Several medicinal plants and formulations known for their therapeutic potential in respiratory ailments were investigated, includingWithania somnifera, Tinospora cordifolia, Glycyrrhiza glabra, and AYUSH-64, an herbal formulation. The gene expression and corresponding secreted levels of various inflammatory mediators were measured using RT-PCR and ELISA methods, respectively, in treated and untreated differentiated THP1 cells induced with TLR7/8 agonists. Comparatively, the gene expression of inflammatory markers was significantly higher in resiquimod-induced cells than in imiquimod-treated cells. Notably,Withania somniferademonstrated pronounced prophylactic efficacy compared to other herbs/formulations, as evidenced by reduction in expression of majority of investigated inflammatory marker genes.
Effect of NaClO4 Dopant on Chemical Bond and Ionic Conductivity of Benzoyl Kappa-carrageenan Gel Biopolymer Electrolyte
Authors: Manap Nor Rahafza Abdul , Shamsudin Intan Juliana , Shrgawi Norsyabila , Kasim Norherdawati , Noor Siti Aminah Mohd , Taufik Safura , Hanibah Hussein 
Published: July 2023

Abstract
Gel biopolymer electrolytes based on benzoyl kappa-carrageenan (Bz-κcar) as polymer host and sodium perchlorate (NaClO4) as dopant was successfully produced. The concentration of NaClO4 was varied from 0.5 to 3.0 wt.% in order to investigate its effects on the chemical bonds and the ionic conductivities (σ) of the electrolytes. Characterizations were performed by Fourier-transform infrared spectroscopy (FTIR) and electrochemical impedance analysis (EIS). Significant changes in the FTIR spectra were detected indicated chemical interactions between Bz-ƙcar and NaClO4. The ionic conductivity of the gel electrolytes increased with higher concentration of NaClO4, suggesting that NaClO4 was an effective charge carrier in the system. The highest σ of the gel electrolyte attained at ambient temperature (298 K) was 1.29 x 10− 3 S cm− 1. The temperature dependence of conductivity is Arrhenian in the studied temperature range and achieved elevated σ of 7.90 x 10− 3 S cm− 1 at 100°C with small values in the activation energy (Ea) were observed in all the electrolytes prepared.
A Bibliometric Analysis of CiteSpace-Based Machine Learning Research in Chinese Medicine
Authors: An Lu , Qi Yingxia , Lin Shuyuan , Liu Chang , Lai Xin , Wang Jue , Yan Peiyu , Lu Liming , Li Yu 
Published: July 2023

Abstract
Artificial intelligence (AI) is widely used in various fields, among which machine learning (ML) is the core of AI that can be rapidly updated and developed. ML has been continuously applied to the field of traditional Chinese medicine (TCM) in the past decades, and it has also attracted more and more attention. This study uses CiteSpace and Excel software to explore the development trends and research hotspots of TCM combined with ML. The Web of Science core database was searched using ML algorithms and TCM. The annual publication volume, country (region), institution, author, journal, cited literature, and keywords were analyzed. The results of the study showed that China contributed the most publications, with 95% of the literature originating from China, followed by the United States. The most prolific institution and authors belonged to the Shanghai University of Traditional Chinese Medicine. Evidence Based Complementary and Alternative Medicine was the most focused publication in this research area. The burst detection by cited literature and keywords shows that convolutional neural networks and tongue images as diagnostic methods are the current research hotspots in this field.
Effectiveness of Monetary Policy Transmission Mechanism Through Interest Rate Channel in Tanzania
Authors: Kimolo Deogratius W. , Naumanga Happiness , Mhoja Angelina , Makoye Elias , Salapion Ladslaus 
Published: July 2023

Abstract
This study investigates the effectiveness of monetary policy transmission through interest rates in Tanzania using quarterly data from 2002 to 2022. The study specifically examines how fluctuations in the monetary policy rate (measured by the repo rate) influence the overnight and 7-day interbank rates, as well as short-term and long-term lending and deposit rates, and consequently, the general price level and the overall economy. In pursuit of these objectives, the empirical analysis employs Autoregressive Distributed Lag (ARDL) to analyze the degree of interest rate pass-through and the Structural Vector Autoregressive (SVAR) model to scrutinize the efficacy of monetary policy transmission mechanism to the overall economy and prices through the interest rate channel. The study finds a complete pass-through of monetary policy rates to interbank market interest rates, with the 7-day interbank rate showing greater responsiveness to changes in policy rates than the overnight interbank rate. The pass-through to short-term deposit rates is statistically significant but low in the short run, with a higher degree than in previous studies indicating improvement in transmission. Furthermore, there is an incomplete pass-through of the policy rate to both short-term and long-term lending rates. In addition, the study finds that the transmission of monetary policy from policy rate to output and prices is weak. The study provides four key policy implications. First, the 7-day interbank rate could potentially serve as an operational target in the conduct of monetary policy. Second, the observed improvement in the transmission process could serve as a basis for supporting the adoption of an interest rate-based monetary policy framework in Tanzania. However, there are still limitations to this framework, and efforts should be made to address them. Third, to improve lending rates sensitivity in Tanzania, efforts should be made to address challenges related to bank-specific, industry-specific, and macroeconomic factors. Finally, efforts should be made to improve financial inclusion, financial market competition, and transparency of the Bank of Tanzania's monetary policy operations to improve monetary policy transmission.
p27 Kip1 and cytoplasmic pSer10p27 are promising biomarkers for predicting prognosis and chemotherapy response in ovarian cancer
Authors: Zhu Mengna , Sun Si , Huang Lin , Gao Lingling , Chen Mengqing , Cai Jing , Wang Zehua , Peng Minggang 
Published: July 2023

Abstract
Purpose The biological function of p27Kip1 largely depends on its subcellular localization and phosphorylation status. Different subcellular localization and phosphorylation status of p27Kip1 may represent distinct clinical values, which are not entirely clear in ovarian cancer. This study aimed to elucidate different subcellular localizations of p27Kip1 and pSer10p27 in predicting prognosis and chemotherapy response in ovarian cancer.
Methods Meta-analyses were executed to evaluate the association of p27Kip1 and phosphorylated p27Kip1 with the prognosis of ovarian cancer patients. The expression levels and patterns of p27Kip1 and pSer10p27 were evaluated by immunohistochemistry (IHC). The correlations between different p27Kip1 states and clinicopathological features as well as prognosis were analyzed. p27Kip1 and pSer10p27 expression level in cisplatin-sensitive and cisplatin-resistant ovarian cancer cell lines were detected using WB. KEGG analysis and WB were performed to evaluate the involved pathways of p27Kip1.
Results Meta-analyses showed that p27Kip1 was associated with significantly better overall survival (OS) in ovarian cancer (HR = 2.14; 95% CI [1.71 - 2.68]) and pSer10p27 was associated with significantly poor OS in mixed solid tumors (HR = 2.56; 95% CI [1.76 - 3.73]) In our cohort of ovarian cancer patients, low total p27Kip1 remained independent risk factors for OS (HR = 2.097; 95% CI [1.121 - 3.922], P = 0.021) and PFS (HR = 2.483; 95% CI [1.364 - 4.518], P = 0.003), while low cytoplasmic pSer10p27 had independent protective effects in terms of OS (HR = 0.472; 95% CI [0.248 - 0.898], P = 0.022) and PFS (HR = 0.488; 95% CI [0.261 - 0.910], P = 0.024). Patients with low total p27Kip1/pSer10p27 and low nuclear p27Kip1 had worse chemotherapy response while patients with low cytoplasmic pSer10p27 expression had better chemotherapy response. The protein levels of p27Kip1 and pSer10p27 were significantly reduced in cisplatin resistant cell lines SKOV3-cDDP and A2780-cDDP and the level of p27Kip1/pSer10p27 was subjective to Akt activation.
Conclusion The present study demonstrates that p27Kip1 and cytoplasmic pSer10p27 are promising biomarkers for predicting prognosis and chemotherapy response in ovarian cancer.
Effects of mindfulness on depression in college students: mediating role of psychological resilience and moderating role of gender
Authors: Zhang Junliang , Zheng Shuang , Hu Zhongzheng , Wang Jingming 
Published: July 2023

Abstract
Objective
A questionnaire was administered to 963 college students to investigate the mediating effect of psychological resilience in the association between mindfulness and college student depression, as well as the moderating role of gender.
Methods
For this study, data was collected between 20 April and 1 May 2023 at three universities in Jiangxi Province using a random sampling method.963 Chinese university students were surveyed using the Adolescent Mindfulness Scale, the Psychological Resilience Scale, and the Depression Self-Rating Scale.SPSS24.0 software was used for correlation analysis and linear regression analysis,and PROCESS v3.4 model 7 was used to analyze this mediated model with moderating effects.
Results
In the mediated effects model, the direct effect of mindfulness on college depression levels was significant (95% CI -0.43, -0.31); the indirect effect of mindfulness on college depression through psychological resilience was also significant (95% CI -0.34, -0.23); the interaction effect of mindfulness and gender was also found to be significant (95% CI 0.03, 0.16) in the mediated model with moderation.
Conclusion
Mindfulness not only directly predicted depression, but also indirectly predicted depression through the mediating effect of psychological resilience ; At the same time, the prediction of psychological resilience by mindfulness was also moderated by gender, with female college students' psychological resilience being more strongly influenced by the level of mindfulness compared to male students.
NADPH and NAC Synergistically Inhibits Chronic Ocular Hypertension-Induced Neurodegeneration and Neuroinflammation through Regulating p38/MAPK Pathway and Peroxidation
Authors: Yu Naiji , Wu Xingdi , Zhang Chengshou , Qin Qiyu , Gu Yuxiang , Ke Weishaer , Liu Xin , Zhang Qi , Chen Min , Wang Kaijun 
Published: July 2023

Abstract

Glaucoma, the leading cause of irreversible blindness worldwide, is characterized by neurodegeneration and neuroinflammation with retinal NAD/NADP and GSH decline. Nicotinamide adenine dinucleotide (NAD)/NAD phosphate (NADP) and glutathione (GSH) are two redox reducers in neuronal and glial metabolism. However, therapeutic strategies targeting NAD/NADP or GSH do not exert ideal effects, and the underlying mechanisms are still poorly understood. We assessed morphological changes inretinal ganglion cells (RGCs), the affected neurons in glaucoma, and Müller cells, the major glial cells in the retina, as well as thelevels of phosphorylated p38 (p-p38) and Caspase-3 in glaucoma patients. We constructed a modified chronic ocular hypertensive rat model and an oxygen-glucose deprivation (OGD) cell model. After applying NADPH and N-acetylcysteine (NAC), a precursor to cysteine, the rate-limiting substrate in GSH biosynthesis, to cells, apoptosis, axonal damage and peroxidation were reduced in the RGCs of the NAC group and p-p38 levelswere decreased in the RGCs of the NADPH group, while in stimulated Müller cells cultured individually or cocultured with RGCs, gliosis and p38/MAPK, rather than JNK/MAPK, activation were inhibited. The results were more synergistic in therat model, where either NADPH or NAC showed crossover effects on inhibiting peroxidation and p38/MAPK pathway activation. Moreover, the combination of NADPH and NAC ameliorated RGC electrophysiological function and prevented Müller cell gliosis to the greatest extent. These data illustrated conjoined mechanisms in glaucomatous RGC injury and Müller cell gliosis and suggested that NADPH and NAC collaborate as a neuroprotective and anti-inflammatory combination treatment for glaucoma and other underlying human neurodegenerative diseases.
Preventive potential of Lactobacillus johnsonii YH1136 against uric acid accumulation and hyperuricemia- induced damages in rats
Authors: Zhang Xingting , Zhao Zhifang , Sun Ning , Xin Jinge , Gan Baoxing , Jiang Yi , Gong Xuemei , Chen Yu , Hu Na , Li Hao , Ma Hailin , Ni Xueqin , Bai Yang , Wang Hesong 
Published: July 2023

Abstract
Background
Hyperuricemia (HUA) is a prevalent metabolic disorder globally, and its development is increasingly associated with intestinal microbiota. Therefore, probiotics have emerged as a potential and safe approach for lowering uric acid (UA) levels. However, effective probiotic strains and the underlying mechanism remain unknown.
Purpose
This study aims to investigate microbiota alterations in each intestinal segment during HUA to identify the most affected segment and potential probiotic strains.
Methods
This study contains two animal experiments. In the first animal experiment, male SD rats were randomly divided into two groups and administered with either 0.5%CMC (Control group) or potassium oxonate (Model group) by oral gavage for an 84-days period. After sacrifice, the whole Gut microbiota was analyzed. In the ssecond animal experiment,Male SD rats were randomly divided into three groups with Control and model groups treated the same as former experiment, while YH1136 group was treated by Lactobacillus johnsonii YH1136 instead (daily amounts of 2×108 CFU).
Results
Whole intestinal diversity was significantly decreased in the model group than in the control, with the most significant decrease in the cecum and colon. Firmicutes, Bacteroidota, and Actinobacteriota were the dominant phyla common to the control and model groups, whereas Bacteroidota was more dominant in the colon of the model group than in the control group. In the RDA analysis, Lactobacillus in the colon and the model group exhibited a strong correlation, suggesting that Lactobacillus may play an important role in hyperuricemia. Consequently, Lactobacillus johnsonii YH1136 was used to assess its preventive effects against HUA. The results showed that Lactobacillus johnsonii YH1136 administration effectively reduced serum UA levels in vivo, mainly inhibiting hepatic xanthine oxidas(XOD) activity and promoting renal ABCG2 transporter expression.Moreover, increased colonization using Lactobacillus johnsonii YH1136 significantly ameliorated pathological damage in the kidney and liver, causing UA accumulation.
Conlusion
These findings highlight the potential significance of Lactobacillus as a connection between HUA and the gut microbiota, providing compelling evidence for Lactobacillus johnsonii YH1136 as a potential treatment for HUA.
Lipopolymer Mediated siRNA Delivery Targeting Aberrant Oncogenes for Effective Therapy of Myeloid Leukemia
Authors: Uludag Hasan , Ansari Aysha , KC Remant , Morales Luis , Nasrullah Mohammad , Nisakar Daniel , Kucharski Cezary , Jiang Xiaoyan , Brandwein Joseph 
Published: July 2023

Abstract
In contrast to exploiting proteins as targets like most conventional drugs and tyrosine kinase inhibitors (TKI), that are rendered futile in the face of emerging drug resistance, RNA Interference (RNAi) exerts its therapeutic action towards disease-driving aberrant genes. To realize the potential of RNAi, the major challenge is to efficiently deliver the therapeutic mediator of RNAi, small interfering RNA (siRNA) molecules. In this study, we explored the feasibility of using aliphatic lipid-grafted polymers (lipopolymers) for the delivery of siRNAs against the FLT3 oncogene in acute myeloid leukemia (AML) and BCR-ABL oncogene in chronic myeloid leukemia (CML). The lipopolymer delivered siRNA potently suppressed the proliferation AML and CML cells via silencing of the targeted oncogenes. In mouse subcutaneous xenograft models, intravenously administered lipopolymer/siRNA complexes displayed significant inhibitory effect on tumor growth. Combining siFLT3 complexes with gilteritinib allowed for reduction of effective drug dosage, longer duration of remission, and enhanced survival after relapse. Anti-leukemic activity of siBCR-ABL complexes was similar in wild-type and TKI-resistant cells, and therapeutic efficacy was confirmed in vivo through prolonged survival of the NCG hosts systemically implanted with TKI-resistant cells. These results demonstrate the preclinical efficacy of lipopolymer facilitated siRNA delivery, providing a novel therapeutic platform for myeloid leukemias.
MYO1E correlates with immune cells infiltration and PD-1/PD-L1 expression in ovarian cancer
Authors: Zhou Xiaomeng , Shi Jiawen , Zhang Xiaojing , Ge Wenliang , Xu Yunzhao 
Published: July 2023

Abstract
Objective
MYO1E is an actin-dependent ATPase that controls tumor development and promotes tumor cell migration and invasion. However, it remains unclear about the relationship between MYO1E and ovarian cancer's clinicopathological characteristics, tumor-infiltrating immune cells, and PD-1/PD-L1.
Methods
Using bioinformatics tools and multiplex immunohistochemistry, we investigated the relationship between MYO1E expression and clinicopathological features of ovarian cancer. Further, we analyzed the relevance between MYO1E expression, tumor-infiltrating immune cells, and PD-1/PD-L1 in ovarian cancer.
Results
There is overexpression of MYO1E mRNA and protein in ovarian cancer compared to the normal ovary and fallopian tube tissue. MYO1E expression is associated with an unfavorable ovarian cancer prognosis in univariate and multivariate Cox regression analyses. Furthermore, we discovered that MYO1E was associated with neutrophils, macrophages, and PD-1/PD-L1 in the ovarian cancer microenvironment.
Conclusions
Our studies confirmed that MYO1E acts as a predictive marker and has potential implications for immunity therapy in ovarian cancer.
Identification and Characterization of Atm Founder Germline Mutation in Brca-negative Breast Cancer Patients of Arab Ethnicity
Authors: Bu Rong , Siraj Abdul K. , Al-Rasheed Maha , Iqbal Kaleem , Azam Saud , Qadri Zeeshan , Haqawi Wael , Tulbah Asma , Al-Dayel Fouad , Almalik Osama , Al-Kuraya Khawla S. 
Published: July 2023

Abstract
Background
Breast Cancer (BC) is the most prevalent malignancy among women worldwide with germline pathogenic variants/likely pathogenic variants (PVs/LPVs) in BRCA1/2 accounting for a large portion of hereditary cases. Recently, heterozygous PVs/LPVs in the ATM serine/threonine kinase or Ataxia-telangiectasia mutated gene (ATM) has been identified as a moderate susceptibility factor for BC in diverse ethnicities. However, the prevalence of ATM PVs/LPVs in BC susceptibility in Arab populations remain largely unexplored.
Methods
This study investigated the prevalence of ATM PVs/LPVs among BC patients from Saudi Arabia, employing capture-sequencing technology for ATM PVs/LPVs screening in a cohort of 715 unselected BC patients without BRCA1/2 PVs/LPVs. In addition, founder mutation analysis was conducted using PHASE program.
Results
In our entire cohort, four unique PVs/LPVs in ATM gene were identified in six cases (0.8%). Notably, one recurrent LPV, c.6115G > A:p.Glu2039Lys, was identified in three cases, for which haplotype analysis confirmed as a novel putative founder mutation traced back to 13 generations on average. This founder mutation accounted for half of all identified mutant cases and 0.4% of total screened cases. This study further reveals a significant correlation between the presence of ATM mutation and family history of BC (p = 0.0127).
Conclusions
These finding underscore an approximate 0.8% prevalence of ATM germline PVs/LPVs in Arab BC patients without BRCA1/2 PVs/LPVs and suggested a founder effect of specific recurrent ATM mutation. These insights can help in the design of a genetic testing strategy tailored to the local population in Saudi Arabia, thereby, enabling more accurate clinical management and risk prediction.
Modeling of Sedimentation of Particles near Corrugated Surface by Meshless Method of Fundamental Solutions
Authors: Povitsky Alex 
Published: July 2023

Abstract
The velocity and trajectory of particle moving along the corrugated (rough) surface under action of gravity is obtained by meshless Method of Fundamental Solutions (MFS). This physical situation is found often in biological systems and microfluidic devices. The Stokes equations with no-slip boundary conditions are solved using the Green function for Stokeslets. In the present study, the velocity of a moving particle is not known and becomes a part of the MFS solution. This requires an adjustment of the matrix of MFS linear system to include the unknown particle velocity and incorporate in the MFS the balance of hydrodynamic and gravity forces acting on the particle. Combination of regularization of Stokeslets and placement of Stokeslets outside flow domain was explored to ensure accuracy and stability of computations for particles moving in proximity to wall. Comparison has been made to prior published approximate analytical and experimental results to verify the effectiveness of this methodology to predict the trajectory of particle including its deviation from vertical trajectory and select the optimal set of computational parameters. The developed MFS methodology is applied to sedimentation of two spherical particles in proximity for which case the analytical solution is not available.