bluuebunny/crossref_metadata_2025_split huggingface dataset:
Polymorphism of genes of circadian rhythms in employees of a continuous production enterprise with a shift work schedule
Authors: Subbotina Alena A. , Dolgikh Oleg V. 
Published: April 2023

The circadian system plays an important role in regulating the functions of the human body. As a result of violations of the circadian system, functional disorders such as sleep disorders, carbohydrate metabolism, metabolic syndrome turning into obesity, cardiovascular pathology, oncological processes occur. One of the socially significant causes of circadian rhythm disorders is a shift work schedule.
The study aims to explore the genetic status of employees and identify markers of predisposition to the formation of "intolerance" of shift work.
Researchers have examined two groups of employees of the enterprise: observation group — 40 people gave 23–63 years old (30 women, 10 men) with a shift work schedule, the comparison group consisted of 51 people 24–62 years old (41 women, 10 men) of the administrative apparatus with a daily work schedule. Typing of polymorphic loci of the circadian system genes was carried out by sets of the company "Syntol" (Russia) using the Real-Time PCR method on a CFX96 device with allelic discrimination of fluorescence curves.
Genotyping of the loci of the five main circadian genes allowed us to establish that the occurrence of the minor allele T of the MTNR1A melatonin receptor gene (rs34532313) in continuous production workers is 30%, which is five times higher than in workers with a standardized work schedule (p<0.05). At the same time, workers with a shift schedule had a significant hypo-expression of serum serotonin (1.54 times, p<0.05) relative to the comparison group.
Continuous production workers have an increased occurrence of the minor allele of the MTNR1A melatonin receptor T gene (rs34532313) with a simultaneous decrease in the expression of serotonin catecholamine, which in shift work conditions can affect the formation of dysregulatory disorders of the functioning of homeostatic systems and the course of physiological metabolic processes. As a result, the polymorphism of the MTNR1A gene (rs34532313) can use as a marker of "intolerance" to shift work.
Limitations. There are quantitative restrictions on the observation group of persons of different genders.
Ethics. When conducting the study, the authors were guided by the Ethical principles of conducting medical research with the participation of a person as a subject, set out in the Helsinki Declaration of the World Medical Association of the last revision. The patients signed a voluntary informed consent for the examination.
Study of the influence of individual components in the cultivation environment on P. aeruginosa and A. baumannii biofilm-forming activity
Authors: Kovalchuk V. P. , Burkot V. M. , Dudar A. O. , Kondratuk V. M. , Zhorniak O. I. , Prokopchuk Z. M. , Trofimenko Y. Y. 
Published: April 2023

Annotation. P. aeruginosa, A. baumannii are the causative agents of severe wound infections, ventilator-associated pneumonia, catheter-associated septic conditions and other severe pathological processes. The ability of bacteria to form biofilms in affected tissues plays an important role in the pathogenesis of these diseases. Therefore, the purpose of our work is to study the influence of certain external factors on the ability of bacteria to form biofilms. The ability of bacteria to form biofilms in affected tissues plays an important role in the pathogenesis of these diseases. 5 clinical strains of P. aeruginosa and 5 strains of A. baumannii were used in this research. The ability to form biofilms was studied in 96-well polystyrene plates by the spectrophotometric method, which is based on the ability of the crystal violet dye to bind to the cells and matrix of the biofilm. Statistical processing of the obtained results was carried out using Excel Microsoft Office tables. The effect of the presence of certain sugars and nitrogen-containing compounds in the cultivation medium on the processes of biofilm formation by pseudomonads and acinetobacteria was investigated. As a result of the research, it was established that acinetobacteria show the ability to form films, unlike pseudomonads, under the condition of their cultivation in sterile distilled water. The introduction of certain carbohydrates or urea into the cultivation medium intensified the biofilm-forming properties of pseudomonads to a much greater extent, compared to acinetobacteria. According to the research results, it was established that the most active process of biofilm formation occurs in both types of non-fermenting Gram-negative bacteria when there are animal blood serum proteins in the culture medium. The formation of biofilms is one of the main strategies that increases the survival of bacteria in the environment, as well as in the human body. The ability of bacteria to form biofilms is, among other things, a factor in the pathogenicity and virulence of bacteria. So, in recent years, special attention has been paid to the ability of microorganisms to form biofilms, and to the study of factors affecting this process.
Endoscopic third ventriculostomy and biopsy of a tectal lesion using flexible neuroendoscopy and urological cup forceps: illustrative case
Authors: Yang Meredith , Wolfson Daniel , LoPresti Melissa A. , Poland Emma , Lam Sandi , DeCuypere Michael 
Published: April 2023

BACKGROUND
Tectal region tumors often clinically present as obstructive hydrocephalus due to mass effect on the outflow of the third ventricle and cerebral aqueduct. Pathology in this region varies; thus, biopsy can be of great value in the management decision making. Appropriate instrumentation remains an area of interest to further advance flexible neuroendoscopic techniques and applications.

OBSERVATIONS
The authors report an illustrative case using flexible neuroendoscopy through a single burr hole for simultaneous endoscopic third ventriculostomy (ETV) and tectal tumor biopsy using urological cup forceps in a 13-year-old boy who had presented with obstructive hydrocephalus.

LESSONS
The authors demonstrate the feasibility of simultaneous ETV and tectal lesion biopsy via flexible neuroendoscopy to address obstructive hydrocephalus and obtain a tissue biopsy in a single-site procedure. They found that the use of flexible cup forceps designed for uroscopy is an important complement to flexible neuroendoscopy. Given the evolving applications of flexible neuroendoscopy, this has implications for instrumentation adaptation and future design.
Cervical radiculopathy due to extracranial vertebral artery dissection treated by stent placement with a flow diversion effect: illustrative case
Authors: Matsumoto Hiroaki , Yamaura Ikuya , Matsumoto Atsushi , Miyata Shiro , Tomogane Yusuke , Minami Hiroaki , Masuda Atsushi , Yoshida Yasuhisa 
Published: April 2023

BACKGROUND
Cervical radiculopathy due to extracranial vertebral artery dissection (VAD) is extremely rare. The disease is usually treated with conservative treatment because of its favorable prognosis. However, there is a possibility that conservative treatment may bring about no improvement in radiculopathy. Although stent placement with a flow diversion effect may be effective in such cases, there are no reported cases that were treated with stent placement.

OBSERVATIONS
A 40-year-old healthy man presented with severe right neck pain, right arm pain, and right arm weakness after cracking his neck. A neurological examination revealed right C5 radiculopathy. Neuroimaging studies revealed right extracranial VAD. The VAD compressed the right C5 nerve root. Although medications were administered, there was no improvement in the symptoms. He experienced severe radicular pain. The authors performed stent placement with a flow diversion effect 10 days after the onset of VAD. His radicular pain improved immediately after the procedure, and the remaining radiculopathy completely improved within 1 month. Follow-up angiography showed complete improvement of the VAD.

LESSONS
Stent placement with a flow diversion effect may be considered when radiculopathy that hinders a patient’s daily life exists. Stent placement may bring about rapid improvement in radiculopathy, especially radicular pain.
Social security agency of health (BPJS health) implementation on dentures patient in USA community health center (Puskesmas USA) bone regency 2022
Authors: Amelia A. Rizki , Aldi Andi Tenri Ummu Dwi Rista Andani , Alwi Muh Khidri , Irfandinata Irfandinata , Amir Haeril 
Published: April 2023

Abstract: Implementation of Social Security Agency of Health (SCAH) /BPJS for Dentures Patients at the Usa Community Health Center in Bone Regency 2022. Tooth loss is a problem that can interfere with dental and oral health. This study aims to explore the implementation of social security agency policies for dentures patients at the Usa Community Health Center in 2022. This type of research is a qualitative study using a snowball sampling technique. The results of this study found that the informants had understood the services for installing dental prostheses that were borne by BPJS Health. As for the information, they do not understand the tariffs for dental prostheses or dentures, which are included in the service being covered by BPJS Health. BPJS Health provides a number of medical services for the community, one of which is dental and oral care, in this case dental prostheses or dentures. From the input and process results it was found that for the implementation of BPJS Health for dental prostheses there are still indicators that are not fully achieved, such as the rates of prostheses or dentures which are still misinterpreted by some patients.
Pengolahan Sampah Organik Menggunakan BSF Dengan Metode Pola Plasma
Authors: Fitriyah Sukhriyatun , Nisa Roifatun 
Published: April 2023

Sampah organik memiliki presentase terbanyak dibandingkan dengan sampah anorganik. berdasarkan Data yang bersumber dari Kementrian Lingkungan Hidup tahun 2021 dari 212 kabupaten/ kota di Indonesia timbulan sampah sebanyak lebih dari 26 juta ton/tahun. Limbah organik yang melimpah setiap harinya dapat diolah dengan berbagai cara. Salah satunya adalah dengan cara memanfaatkannya sebagai media budidaya ulat/maggot lalat BSF (Black Soldier Fly). Masyarakat di Desa Singaraja Kabupaten Indramayu masih banyak yang belum memahami cara pengolahan sampah organik menggunakan BSF. Penelitian ini bertujuan ingin mengetahui pengetahuan masyarakat mengenai sampah dan BSF serta membuat metode pengelolaan sampah organik menggunakan BSF dengan cara pola plasma. Inti pola dalam penelitian ini yaitu Bank Sampah yang ada di Desa Singaraja dan plasma pengelolaan sampah organik dengan BSF nya dibuat di 12 titik dimana masing-masing titik tersebut dibuat 2-3 plasma. Penelitian ini menggunakan penelitian eksperimen semu (quasi experiment) dengan pendekatan non randomized control group pretest postest design. Sampel dalam penelitian ini sebanyak 30 responden menggunakan metode purposive sampling dengan kriteria inklusi dan eksklusi. Hasil penelitian diketahui hasil pretest dan posttest menunjukkan adanya perbedaan yang signifikan setelah dilakukan pelatihan. Pengetahuan sampah sebelum dan sesudah pelatihan meningkat dimana p-value 0,043 dan pengetahuan BSF ada peningkatan sebelum dan sesudah pelatihan dimana nilai p-value 0,017. Secara umum, hasil kegiatan ini memberikan alternatif baru untuk membuat pengolahan sampah organik sehingga lebih baik lagi dalam menangani permasalahan sampah yang bersumber dari rumah tangga.
Neuroinflammation: A Common Pathway in Alzheimer’s Disease and Epilepsy
Authors: Liew Yee , Retinasamy Thaarvena , Arulsamy Alina , Ali Idrish , Jones Nigel C. , O’Brien Terence J. , Shaikh Mohd Farooq 
Published: April 2023

Background: Neuroinflammation is an innate immunological response of the central nervous system that may be induced by a brain insult and chronic neurodegenerative conditions. Recent research has shown that neuroinflammation may contribute to the initiation of Alzheimer’s disease (AD) pathogenesis and associated epileptogenesis. Objective: This systematic review aimed to investigate the available literature on the shared molecular mechanisms of neuroinflammation in AD and epilepsy. Methods: The search included in this systematic review was obtained from 5 established databases. A total of 2,760 articles were screened according to inclusion criteria. Articles related to the modulation of the inflammatory biomarkers commonly associated with the progression of AD and epilepsy in all populations were included in this review. Results: Only 7 articles met these criteria and were chosen for further analysis. Selected studies include both in vitro and in vivo research conducted on rodents. Several neuroinflammatory biomarkers were reported to be involved in the cross-talk between AD and epilepsy. Conclusion: Neuroinflammation was directly associated with the advancement of AD and epilepsy in populations compared to those with either AD or epilepsy. However, more studies focusing on common inflammatory biomarkers are required to develop standardized monitoring guidelines to prevent the manifestation of epilepsy and delay the progression of AD in patients.
Depression in Mid- and Later-Life and Risk of Dementia in Women: A Prospective Study within the Danish Nurses Cohort
Authors: Hickey Martha , Hueg Trine K. , Priskorn Lærke , Uldbjerg Cecilie S. , Beck Astrid L. , Anstey Kaarin J. , Lim Youn-Hee , Bräuner Elvira V. 
Published: April 2023

Background: Depression and dementia confer substantial global health burdens, particularly in women. Understanding the association between depression and dementia may inform new targets for prevention and/or early intervention. Objective: To investigate the association between depression in mid- and later-life and dementia (all-cause, Alzheimer’s disease (AD) or vascular dementia (VaD)) in women. Methods: A prospective study design. Nurses were followed from age 60 years or entry into the cohort, whichever came last, until date of dementia, death, emigration, or end of follow-up, whichever came first. Cox regression models with age as the underlying timeline were used to estimate the associations between time-varying depression and incident dementia. Results: The study included 25,651 female Danish nurses (≥45 years) participating in the Danish Nurse Cohort. During an average of 23 years of follow-up, 1,232 (4.8%) nurses developed dementia and 8,086 (31.5%) were identified with at least two episodes of treated depression. In adjusted analyses, nurses with depression were at a statistically significant 5.23-fold higher risk of all-cause dementia (aHR 5.23:95% CI, 4.64–5.91) compared to those with no history of depression. The differential effects of depression were greater for VaD (aHR 7.96:95% CI, 5.26–12.0) than AD (aHR 4.64:95% CI, 3.97–5.42). Later life depression (>60 years) (aHR 5.85:95% CI, 5.17–6.64) and recurrent depression (aHR 3.51:95% CI, 2.67–4.61) elevated dementia risk. Severe depression tripled the risk of all cause dementia (aHR 3.14:95% CI, 2.62–3.76). Conclusion: Both later life and severe depression substantially increase dementia risk in women, particularly VaD.
Patients with Spinal Muscular Atrophy Type 1 Achieve and Maintain Bulbar Function Following Onasemnogene Abeparvovec Treatment
Authors: McGrattan Katlyn E. , Shell Richard D. , Hurst-Davis Rebecca , Young Sally Dunaway , O’Brien Eamonn , Lavrov Arseniy , Wallach Shiri , LaMarca Nicole , Reyna Sandra P. , Darras Basil T. 
Published: April 2023

Background: Improvement and maintenance of bulbar function are goals of disease-modifying treatments for spinal muscular atrophy (SMA). Lack of standardized measures and a widely accepted definition of bulbar function represents a gap in SMA care. Objective: A multidisciplinary team conducted post-hoc analyses of pooled data from one phase 1 (START) and two phase 3 (STR1VE-US, STR1VE-EU) studies to define and evaluate bulbar function of infants with SMA type 1 after receiving one-time gene replacement therapy, onasemnogene abeparvovec. Methods: We defined bulbar function as the ability to meet nutritional needs while maintaining airway protection and the ability to communicate verbally. Four endpoints represented adequate bulbar function: (1) absence of clinician-identified physiologic swallowing impairment, (2) receiving full oral nutrition, (3) absence of adverse events indicating pulmonary instability, and (4) the ability to vocalize at least two different, distinct vowel sounds. We descriptively assessed numbers/percentages of patients who achieved each endpoint and all four collectively. Patients were followed until 18 months old (STR1VE-US and STR1VE-EU) or 24 months (START) post-infusion. Results: Overall, 65 patients were analyzed for swallowing, nutrition intake, and adverse events, and 20 were analyzed for communication. At study end, 92% (60/65) of patients had a normal swallow, 75% (49/65) achieved full oral nutrition, 92% (60/65) had no evidence of pulmonary instability, 95% (19/20) met the communication endpoint, and 75% (15/20) achieved all four bulbar function components in the composite endpoint. Conclusions: In these three clinical trials, patients with SMA type 1 who received onasemnogene abeparvovec achieved and maintained the bulbar function criteria utilized within this investigation.
Structural-Functional Correlates of Response to Pedunculopontine Stimulation in a Randomized Clinical Trial for Axial Symptoms of Parkinson’s Disease
Authors: Breit Sorin , Milosevic Luka , Naros Georgios , Cebi Idil , Weiss Daniel , Gharabaghi Alireza 
Published: April 2023

Background: Axial symptoms of Parkinson’s disease (PD) can be debilitating and are often refractory to conventional therapies such as dopamine replacement therapy and deep brain stimulation (DBS) of the subthalamic nuclei (STN). Objective: Evaluate the efficacy of bilateral DBS of the pedunculopontine nucleus area (PPNa) and investigate structural and physiological correlates of clinical response. Methods: A randomized, double-blind, cross-over clinical trial was employed to evaluate the efficacy of bilateral PPNa-DBS on axial symptoms. Lead positions and neuronal activity were evaluated with respect to clinical response. Connectomic cortical activation profiles were generated based on the volumes of tissue activated. Results: PPNa-DBS modestly improved ( p = 0.057) axial symptoms in the medication-off condition, with greatest positive effects on gait symptoms ( p = 0.027). Electrode placements towards the anterior commissure (ρ= 0.912; p = 0.011) or foramen caecum (ρ= 0.853; p = 0.031), near the 50% mark of the ponto-mesencephalic junction, yielded better therapeutic responses. Recording trajectories of patients with better therapeutic responses (i.e., more anterior electrode placements) had neurons with lower firing-rates ( p = 0.003) and higher burst indexes ( p = 0.007). Structural connectomic profiles implicated activation of fibers of the posterior parietal lobule which is involved in orienting behavior and locomotion. Conclusion: Bilateral PPNa-DBS influenced gait symptoms in patients with PD. Anatomical and physiological information may aid in localization of a favorable stimulation target.
Rhythmic Oscillations and Resonant Information Transfer in Biological Macromolecules
Authors: Brown William , Meijer Dirk 
Published: April 2023

This manuscript is a concise review of a selection of key concepts concering _rythmic oscillations in proteins to human cognition_. All matter has an associated frequency, characterized by υ_ = (mc2)/h _(derived from the equivalency _E = mc2 + pc_, and _E = hυ_). In large organic macromolecules and biopolymers of the living system, there are multiple vibrational modes and oscillatory frequencies, the resonances of which can facilitate molecular recognition, coupling with field modes, electromagnetic and Van der Waals interactions. Via harmonic rhythmic oscillations and resonances nanosecond-scale intermolecular interactions are highly coordinated to orchestrate the myriad complex biochemistry pathways of the cell. Importantly, this field-like interaction is integral in information processing and exchange occurring at the molecular level that underlie cellular intelligence, network intelligence, and perhaps even sentience and consciousness. We will review here the _resonant recognition model _of Irena Cosic and the scale-invariant acoustic information code of a superfluid quantum space described by Meijer.
Discordance between Criteria-diagnosed and clinically-diagnosed Asthma COPD Overlap (ACO) among hospitalized patients in an Indian referral hospital.
Authors: Daniel Jefferson , Isaac Barney , Christopher Devasahayam Jesudas 
Published: April 2023

Background and Objectives: Asthma-COPD-overlap (ACO), as a single entity, has been widely contested over the past few years. Most ACO diagnostic criteria do not agree with each other when applied to respiratory patients. From 2016 to 2020, GINA described a syndromic approach for diagnosing ACO, which was replaced with a broader approach after 2021. We intended to learn if the GINA criteria and the CSMA Screening tool agreed with a clinical diagnosis of ACO. Methods: We performed a retrospective cross-sectional analysis of inpatients with a clinical diagnosis of ACO from 2014 to 2019. We reclassified these patients into Not-ACO and ACO using the GINA syndromic approach 2019 (GSA 2019), Modified GINA syndromic approach 2019 (MGSA 2019), GINA 2021 and the CSMA tool. We used Kappa statistics to compare the performance of various tools. Results: 83 clinically diagnosed ACO patients were included. According to GINA syndromic approach 2019, only 41 (49.39%) patients would be classified as ACO. GINA 2021 picked up 57 (68.67%) patients as ACO. The modified GINA syndromic approach 2019, which we proposed, identified 67 (80.72%) patients as ACO. The CSMA tool identified 63 (75.90%) patients as ACO. The GINA 2019 criteria, when interpreted liberally as described by us, have a better agreement with the CSMA tool with a sensitivity of 87.3% and specificity of 40%, with a 'k' agreement of 29.3%. Conclusion: The standard GINA syndromic approach 2019 Tool is stringent and may sometimes miss the ACO diagnosis. The original GINA syndromic approach table 2019 should be brought back with modifications.