bluuebunny/crossref_metadata_2025_split huggingface dataset:
THE DYNAMICS OF FAMILY-OWNED SME SUSTAINABILITY IN RURAL AREAS [DINAMIKA KEBERLANJUTAN UKM KELUARGA DI PEDESAAN]
Authors: Kusmulyono Muhammad Setiawan 
Published: September 2023

<p><em>SMEs in rural areas have characteristics that are closely related to the character of a family business. This can be seen in the management of the SME business which is fully controlled by the family. This situation shows that SMEs in rural areas are more dominated by family-owned SMEs. As a family-owned SME, this village entrepreneur has a big challenge to be able to regenerate the business for the next generation. This study seeks to identify the things that affect the dynamics in realizing the sustainability of family-owned SMEs in the village. This study uses a qualitative approach by conducting interviews through purposive sampling to culinary informants who run their businesses in rural areas and have the next generation. The results of the study show that in addition to all SMEs in the village being dominated by family businesses, these SMEs have hopes that their families will continue their business, but on the other hand it was also found that there are SMEs who do not care who will continue their business. This study produces interesting findings that can be developed to help improve the sustainability of SME development in Indonesia.</em></p><p> </p><p><strong>Abstrak dalam Bahasa Indonesia.</strong> UKM di pedesaan memiliki karakteristik yang erat kaitannya dengan karakter usaha keluarga. Hal ini terlihat dari pengelolaan usaha UKM yang sepenuhnya dikendalikan oleh keluarga. Keadaan ini menunjukkan bahwa UKM di pedesaan lebih didominasi oleh UKM milik keluarga. Sebagai UKM milik keluarga, pengusaha desa ini memiliki tantangan besar untuk bisa meregenerasi bisnisnya untuk generasi selanjutnya. Kajian ini berupaya mengidentifikasi hal-hal yang mempengaruhi dinamika dalam mewujudkan keberlanjutan UKM milik keluarga di desa. Penelitian ini menggunakan pendekatan kualitatif dengan melakukan wawancara melalui purposive sampling kepada informan kuliner yang menjalankan usahanya di pedesaan dan memiliki generasi penerus. Hasil kajian menunjukkan bahwa selain seluruh UKM yang ada di desa didominasi oleh usaha keluarga, UKM ini memiliki harapan agar keluarganya tetap melanjutkan usahanya, namun disisi lain juga ditemukan adanya UKM yang tidak peduli yang akan melanjutkan usahanya. Kajian ini menghasilkan temuan menarik yang dapat dikembangkan untuk membantu meningkatkan keberlanjutan pengembangan UKM di Indonesia.</p><p> </p>
KNOWLEDGE AND ATTITUDE OF FARMERS TOWARDS ANTIMICROBIAL RESISTANCE IN ASIA: A SYSTEMATIC REVIEW AND META-ANALYSIS
Authors: Mikail Athif Zhafir Asyura Muhammad , Angela Novia 
Published: September 2023

Background: Antimicrobial resistance is a severe threat to public and environmental health. The agricultural sector contributes significantly to resistance, where antimicrobials are used as prophylaxis, growth promoters, and for treatment. A series of studies have been conducted to assess farmers' knowledge and attitude levels with varying results, particularly in Asia, one of the world's largest producers of livestock products. Purpose: To review the pooled estimated level of knowledge and attitude towards antimicrobial use and resistance in Asia. Methods: A literature search was conducted according to PRISMA in Scopus, PubMed, Google Scholar, and Embase for studies up to 30 April 2023. Quality was assessed using the Newcastle-Ottawa Scale (NOS) for cross-sectional studies. Outcomes were further categorized into constructs under knowledge and attitude. Random-effect meta-analysis was conducted using STATA 17. Results: 11 studies and 2131 subjects were included with fair to excellent quality. From the meta-analysis, the following knowledge and attitude levels were estimated: definition [55.7% (95%CI: 37.3%-74%)] and cause [60.6% (95%CI: 40.5%-80.6%)] of antimicrobial resistance; the negative impact of antimicrobials [62.6% (95%CI: 16.9%-100.0%)]; use of antimicrobials for treatment [47.8% (95%CI: 6.1%-89. 4%)], prophylaxis [58.5% (95%CI: 28.5%-88.5%)], growth promoter [39% (95%CI: 23.1%-54.9%)]; discontinuation of antimicrobials upon improving conditions [42.5% (95%CI: 15.4%-69.5%)]. Conclusions: Farmers in Asia have moderate knowledge of antimicrobial resistance but still exhibit attitudes that support resistance.
Teknik Self-Management untuk Menurunkan Perilaku Membolos pada Siswa Kelas XI
Authors: Rahma Eriska , Oktavia Citrawanti , Sholichah Ima Fitri 
Published: September 2023

<p>This study aims to determine the effect of <em>self-management</em> techniques on students' behaviour. This research was carried out on class XI students in Islamic high school. This study uses one pretest-posttest design group without comparison groups or control groups, the population employed by class XI IA. A total of 5 students obtained as many as four students. Sampling using a purposive sampling technique. Data collection methods use trooting behaviour questionnaires. Data analysis techniques use parametric paired sample t-test analysis. The results of the study. It shows that <em>self-management</em> techniques reduce the behaviour of skipping students. This is evidenced by the difference in lowering behaviour of diving between the pre-test and post-test results, where the average post-test results are lower than the pre-test results after a <em>self-management</em> technique is given. Based on the results of the pre-test and the existing post-test showed a change in the behaviour. Who experienced a decrease in the conduct of skipping after individual counselling using the behaviour approach with <em>self-management</em> techniques </p>
Public Insurance and Single-Guardian Households Are Associated with Diagnostic Delay in Slipped Capital Femoral Epiphysis
Authors: Smith Lacey M. , Chang Yuchiao , Feldman Candace H. , Santacroce Leah M. , Earle Madison , Katz Jeffrey N. , Novais Eduardo N. 
Published: September 2023

Background:
Extensive literature documents the adverse sequelae of delayed diagnosis of slipped capital femoral epiphysis (SCFE), including worsening deformity and surgical complications. Less is known about predictors of delayed diagnosis of SCFE, particularly the effects of social determinants of health. The purpose of this study was to evaluate the impact of insurance type, family structure, and neighborhood-level socioeconomic vulnerability on the delay of SCFE diagnosis.

Methods:
We reviewed medical records of patients who underwent surgical fixation for stable SCFE at a tertiary pediatric hospital from 2002 to 2021. We abstracted data on demographic characteristics, insurance status, family structure, home address, and symptom duration. We measured diagnostic delay in weeks from the date of symptom onset to diagnosis. We then geocoded patient addresses to determine their Census tract-level U.S. Centers for Disease Control and Prevention (CDC) and Agency for Toxic Substances and Disease Registry (ATSDR) Social Vulnerability Index (SVI), using U.S. Census and American Community Survey data. We performed 3 separate logistic regression models to examine the effects of (1) insurance status, (2) family structure, and (3) SVI on a delay of ≥12 weeks (reference, <12 weeks). We adjusted for age, sex, weight status, number of siblings, and calendar year.

Results:
We identified 351 patients with SCFE; 37% (129) had a diagnostic delay of ≥12 weeks. In multivariable logistic regression models, patients with public insurance were more likely to have a delay of ≥12 weeks than patients with private insurance (adjusted odds ratio [OR], 1.83 [95% confidence interval (CI), 1.12 to 2.97]; p = 0.015) and patients from single-guardian households were more likely to have a delay of ≥12 weeks than patients from multiguardian households (adjusted OR, 1.95 [95% CI, 1.11 to 3.45]; p = 0.021). We did not observe a significant increase in the odds of delay among patients in the highest quartile of overall SVI compared with patients from the lower 3 quartiles, in both the U.S. comparison (adjusted OR, 1.43 [95% CI, 0.79 to 2.58]; p = 0.24) and the Massachusetts comparison (adjusted OR, 1.45 [95% CI, 0.79 to 2.66]; p = 0.23).

Conclusions:
The delay in diagnosis of SCFE remains a concern, with 37% of patients with SCFE presenting with delay of ≥12 weeks. Public insurance and single-guardian households emerged as independent risk factors for diagnostic delay. Interventions to reduce delay may consider focusing on publicly insured patients and those from single-guardian households.

Level of Evidence:
Prognostic Level III. See Instructions for Authors for a complete description of levels of evidence.
Cellular and Molecular Characterization of CADASIL Using In Vitro Models of Vascular Smooth Muscle Cells and Blood Vessel Organoids
Authors: Do Jeong Tae , Lee So Been , Nam Hyo Suk , Oh Seungyeon , Na Seungbin , Kim Hyun Kyu , La Hyeonwoo , Lee Man Ryul , Hong Kwonho 
Published: September 2023

Abstract
Cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy (CADASIL) is the most common inherited form of stroke, characterized by a NOTCH3 mutation on chromosome 19. Despite many genetic and functional studies, the exact mechanisms and pathologies have not been fully elucidated. Herein, the cellular and molecular characteristics of vascular smooth muscle cells (VSMCs) and constituent cells of blood vessel organoids are investigated. Fibroblasts derived from a patient with CADASIL are reprogrammed into iPSCs and differentiated into VSMCs, cerebral organoids, and blood vessel organoids. In CADASIL iPSC-derived VSMCs, NOTCH3 extracellular domain antibody is detected. In CADASIL-blood vessel organoids, significant differences in the blood vessel related parameters between CADASIL and control blood vessel organoids are found. Additionally, cerebral organoids by CADASIL-iPSCs are distinct from those by control-iPSCs. Furthermore, based on RNA sequencing, differentially expressed genes between control and CADASIL samples are associated with proliferation rate, cell cycle, and nuclear division in VSMCs and angiogenesis and vasculogenesis in blood vessel organoids. Six distinct clusters in blood vessel organoids and cell proportion differences between control and CADASIL are identified using single-cell RNA sequencing. This study highlights CADASIL VSMCs and 3D organoids may facilitate investigating molecular and cellular mechanisms underlying CADASIL pathogenesis.
Annual Solar Geoengineering: Mitigating Yearly Global Warming Increases
Authors: Feinberg Alec 
Published: September 2023

Abstract
Solar geoengineering (SG) solutions have many advantages compared to the difficulty of carbon removal (CR): SG produces fast results, is shown here to have much higher efficiency than CR, is not related to fossil fuel legislation, and is something we all can participate in brightening the Earth with cool roofs, and roads. SG requirements detailed previously to mitigate global warming (GW) have been concerning primarily because of overwhelming goals and climate circulation issues. In this paper, the advantages of annual solar geoengineering (ASG) to mitigate yearly global warming increases are explored and detailed as it provides higher feasibility in geoengineering applications. ASG area modification requirements found here are generally 50 to possibly higher than 150 times less compared to the challenge of full SG GW mitigation reducing circulation concerns. Results indicate that there are mixed technologies that can help meet annual mitigation Earth brightening goals. As well, results show much higher feasibility for L1 space shading compared to prior literature estimates for full GW mitigation. However, stratosphere injections appear challenging in the annual approach. Because ASG earth brightening area requirements are much smaller than those required for full mitigation, we have concerns that worldwide negative SG would interfere with making positive advances for several reasons. Negative SG currently dominates yearly practices with the application of dark asphalt roads and roofs. This issue is discussed.
Development and validation of a nomogram to predict the risk of postmenopausal uterine leiomyoma degeneration: A retrospective study
Authors: WANG Yaping , Zhao Yiyi , Shi Chaolu , Li Juanqing , Huang Xiufeng 
Published: September 2023

Abstract
Objective: The aim of this study was to investigate the risk factors for postmenopausal uterine leiomyoma degeneration and to develop a nomogram for clinical risk assessment.
Study design and setting: A total of 508 patients were enrolled from 1 August 2012 to 1 August 2022. Univariate and multivariate logistic regression models were used to analyse the association between variables and uterine leiomyoma degeneration in postmenopausal patients. A nomogram for uterine leiomyoma degeneration in postmenopausal patients was developed and validated by bootstrap resampling. The calibration curve was used to assess the accuracy of the model and the ROC, DCA and NRI were compared with the clinical experience model.
Results: DM, breast cancer, number, size5, LDH, TG and Ca2+ were independent risk factors for uterine leiomyoma degeneration in postmenopausal patients. We developed a user-friendly nomogram which showed good diagnostic performance (AUC=0.714). The model was consistent and the calibration curve of our cohort was close to the ideal diagonal line. DCA indicated that the model has potential value for clinical application. Furthermore, our model was superior to the previous clinical experience model in terms of ROC (P=0.00039), DCA and NRI (NRI=0.4175722).
Conclusions: We have developed a prediction nomogram for uterine leiomyoma degeneration in postmenopausal patients. This nomogram could serve as an important warning signal and evaluation method for the degeneration and malignant transformation of uterine leiomyoma in postmenopausal patients.
Comparative study of the corneal morphological characteristics of keratoconus evaluated using CASIA2, Sirius, and Pentacam
Authors: Zhou Xingtao , Sun Bingqing , Hu Xiaojun , Peng Xiaoliao , Ten Weijung , Huang Yangyi , Sun Ling , Zhang Xiaoyu 
Published: September 2023

Abstract
Purpose
To analyze the corneal morphology of keratoconus using CASIA2, Sirius, and Pentacam.
Methods
Patients diagnosed with keratoconus at the Eye and ENT Hospital of Fudan University (January-November 2022) were included. Corneal refraction, astigmatism, thickness, and elevation parameters were measured using CASIA2, Sirius, and Pentacam. The measurement differences, agreement, and the sensitivity and specificity of each instrument for diagnosing keratoconus was evaluated. P < 0.05 was considered statistically significant.
Results
A total of 61 keratoconus eyes and 48 healthy myopic eyes were included. The Ks and Kf measured using Sirius were the highest (p < 0.0001). The thinnest corneal thickness measured using Pentacam was the thickest (p < 0.0001); however, the significance disappears in the Kmax ≤ 48D keratoconus group. When the best fit sphere was based on CASIA2, the anterior and posterior elevations at thinnest points measured using Sirius were the highest in keratoconus group and the lowest in control group. Corneal refractive power measurements significantly differed among instruments (p < 0.0001). In the control group, the agreement limits for total corneal refractive power (TCRP), fKm, and pKm were lower and narrower than in keratoconus. In keratoconus group, Pentacam and CASIA2 showed the best consistency in the measurement of TCRP and fKm, whereas Pentacam and Sirius showed the best consistency in the measurement of pKm. Sensitivities for diagnosing keratoconus ranked from high to low: Pentacam, CASIA2, Sirius (p < 0.0001).
Conclusion
CASIA2, Sirius, and Pentacam exhibit differences in corneal elevation, refractive power, and thickness measurements, with poor consistency in refractive power measurement.
Fully automated 4D cardiac modelling from 2D echocardiography for clinical applications
Authors: Laumer Fabian , Rubi Lena , Matter Michael , Buoso Stefano , Fringeli Gabriel , Mach Francois , Ruschitzka Frank , Buhmann Joachim , Matter Christian 
Published: September 2023

Abstract
Transthoracic Echocardiography (TTE) is a crucial tool for assessing cardiac morphology and function quickly and non-invasively without ionizing radiation. However, the examination is subject to intra- and inter-user variability and recordings are often limited to 2D imaging and assessments of end-diastolic and end-systolic volumes. Current 3D TTE is time-consuming and provides lower image quality than 2D TTE. We have developed a novel, fully automated machine learning-based framework to generate a 4D (3D plus time) personalized model of the left ventricular (LV) blood pool. This digital twin was generated from specific 2D echocardiographic views employing deep neural networks, pre-trained on a synthetic dataset, and fine-tuned in a self-supervised manner using a novel optimization method for cross-sectional imaging data. This 4D model enabled detailed three dimensional analyses of LV systolic and diastolic function with high temporal resolution. Validation was performed on a multi-center dataset encompassing TTE exams of 144 patients with normal TTEs (controls) and 314 patients with acute myocardial infarction (AMI). LV volume measurements in controls derived from the digital twin showed a high correlation with the values from the clinical TTE reports (Pearson correlation coefficient of 0.7). In AMI patients, these novel biomarkers showed a high predictive value for survival (area under the curve (AUC) of 0.82 for 1-year all-cause mortality) and and excellent diagnostic value for infarct localisation and quantification, bearing the potential to improve diagnostic accuracy and risk assessment. Thus, our digital twin allows for fully automated robust LV shape analyses over time including contractility, relaxation, and regional wall motion abnormalities aiming at replacing expensive 3D imaging modalities with cheaper and more versatile 2D cardiac ultrasound.
Efficacy of four indigenous Penicillium fungal isolates and Aspergillus fumigatus on Fusarium oxysporum f.sp. radicis lycopersici (FORL)
Authors: Ramachela Khosi , Ramatsitsi Mukondeleli Ndivhuwo , Segone Galaletsang Petronella 
Published: September 2023

Abstract

In search for indigenous soil borne fungi with bio-control properties against plant pathogenic F. oxysporum f.sp. radicis-lycopersici (FORL) resulted in the isolation of five fungal species. These were identified as: Penicillium commune-1 (MK660351.1), P. commune-2 (MK660335.1), Aspergillus fumigatus (MN178806.1), P. concavorugulosum-1 (MK841454.1) and P. concavorugulosum-2 (MK841454.1). An in vitro dual culture plate experiment was carried out to assess radial growth inhibition and growth competitiveness of potential antagonism on FORL. Analysis of the fungal bio-agents’ interactions with FORL showed occurrence of various relationships such as antibiosis, competition and mycoparasitism. High inhibition effect of P. concavorugulosum-2 (67.9%) and A. fumigatus (65.36%) against FORL was established. The other test isolates’ inhibition was not significant as they ranged from 9–29%. A conclusion can be drawn that P. concavorugulosum-2 and A. fumigatus contain potential bio-antagonistic properties that suppressed of growth of FORL. These fungal isolates should be considered as good candidates for development as commercial bio-control agents as individuals or in combination. However, these species’ suppressive properties should be evaluated under natural soil conditions where various biotic and abiotic factors could influence the efficacy.
Third-Party Cytotoxic T Lymphocytes for High-Risk Patients with Covid-19
Authors: Grosso Dolores , Wagner John , O'Connor Allyson , Keck Kaitlyn , Huang Yanping , Wang Zi-Xuan , Mehler Hilary , Leiby Benjamin , Flomenberg Phyllis , Gergis Usama , Nikbakht Neda , Morris Michael , Karp Julie , Peedin Alexis , Flomenberg Neal 
Published: September 2023

Abstract
Treatment with off-the-shelf cellular therapy may provide direct and rapid treatment for COVID-19, overcoming the delayed adaptive immune responses associated with poor outcomes in high-risk patients. Thirty ambulatory patients with COVID-19 were enrolled on a phase I trial to assess the safety of 3rd party, COVID-19-specific cytotoxic T lymphocytes (CTLs). Twelve “Interventional” patients matching the HLA-A*02:01 restriction of the CTLs received a single infusion of one of four escalating doses of a product containing 68.5% COVID-19-specific CD8+ CTLs/total cells. Eighteen “Observational” patients lacking HLA-A*02:01 served as comparisons. No dose-limiting toxicities were observed. Nasal swab PCR data showed ≥ 88% viral elimination in 92% of patients in 4 days and the CTLs remained detectable at 6 months. Interventional patients consistently reported symptomatic improvement 2-3 days after infusion, whereas improvement was more variable in Observational patients. Our study shows that COVID-19-specific CTLs are a potentially useful cellular therapy for COVID-19.
Opioid induced hyperalgesia treated with low dose naltrexone improves pain
Authors: Johnson Brian , Zhang-James Yanli 
Published: September 2023

Abstract
Opioids are prescribed for 5 – 8 million Americans with chronic pain1 and many more worldwide. For patients currently on chronic opioid therapy (COT) continuation of opioids is recommended because of mental health crises and overdoses that occur during medication taper2 and because of deaths associated with accidental overdose when opioid medications are discontinued.3, 4 Because opioid medications are exogenous opioid hormones that downregulate the pain damping opioid receptor system and upregulate pain drivers, opioid induced hyperalgesia (OIH) is a near-universal finding when the cold pressor test (CPT) is used to measure pain tolerance.5 Detoxification is best accomplished with a single dose of buprenorphine immediately followed with low dose naltrexone (LDN). LDN treats OIH.
We conducted a retrospective chart review of patients treated on our pain service from 2011 through 2022. Short CPTs improved with detoxification and LDN. Pain diminished.
Our results show that opioid medications are contraindicated for chronic pain. Patients currently prescribed daily opioid medications can safely be detoxified and have their centrally mediated pain treated with LDN, using the CPT to track resolution of OIH. Psychotherapy is necessary in many instances because opioids attenuate feelings and distort relationships.
Characterization of N-methyltransferase for catalyzing the terminus of leucinostatins in Purpureocillium lilacinum
Authors: Li Yan , Li Zixin , Jiao Yang , Ling Jian , zhao Jianlong , Yang Yuhong , Mao Zhenchuan , Zhou Kaixiang , Wang Wenzhao , Xie Bingyan 
Published: September 2023

Abstract

N-methyltransferase (NMT)-catalyzed methylations are rarely reported at nonribosomal peptides (NRPs) terminuses. Here, we discovered a fungal NMT LcsG for the iterative terminal N-methyl formation of a family of NRPs, leucinostatins. Gene deletion suggested LcsG is essential to the methylation of leucinostatins. In vitro assay and HRESI-MS-MS analysis proved the methylation sites were the NH2, NHCH3 and N(CH3)2 in the C-terminal unit of various leucinostatins. Based on the protein structure predicted by artificial intelligence (AI), molecular docking, and site-directed mutagenesis, we proposed the catalytic mechanism of the LcsG-catalyzed reaction was an N atom coordinated by two negatively charged residues (Asp368, Asp395 for LcsG) towards the subsequent SN2 methylation. These findings not only provide an approach for enriching the variety of natural bioactivity of NPRs but also deepen the insight into the catalytic mechanism of N-methylation of NRPs.