bluuebunny/crossref_metadata_2025_split huggingface dataset:
Implementation of a Best Practice Advisory to Improve Infection Screening Prior to New Prescriptions of Biologics and Targeted Synthetic Drugs
Authors: Baker Hailey , Fine Rebecca , Suter Fenn , Allore Heather , Hsiao Betty , Chowdhary Vaidehi , Lavelle Elizabeth , Chen Ping , Hintz Richard , Suter Lisa G. , Danve Abhijeet 
Published: June 2023

ObjectiveUse of biologic and targeted synthetic disease‐modifying antirheumatic drugs (b/tsDMARDs) in patients with preexisting tuberculosis (TB), hepatitis B virus (HBV), or hepatitis C virus (HCV) infection can have serious consequences. Although various society guidelines recommend routine screening for these infections before initiating certain b/tsDMARDs, adherence to these recommendations varies widely. This quality improvement initiative evaluated local compliance with screening and assessed whether an automated computerized decision support system in the form of a best practice advisory (BPA) in the electronic health record could improve patient screening.MethodsEstablished patients with autoimmune rheumatic disease (ARD) aged 18 years or older with at least one visit to our rheumatology practice between October 1, 2017, and March 3, 2022, were included. When prescribing a new b/tsDMARD, clinicians were alerted via a BPA that showed the most recent results for TB, HBV, and HCV. Screening proportions for TB, HBV, and HCV before BPA initiation were compared with those of eligible patients after the BPA implementation.ResultsA total of 711 patients pre‐BPA and 257 patients post‐BPA implementation were included in the study. The BPA implementation was associated with statistically significant improvement in screening for TB from 66% to 82% (P ≤ 0.001), HCV from 60% to 79% (P ≤ 0.001), hepatitis B core antibody 32% to 51% (P ≤ 0.001), and hepatitis B surface antigen from 51% to 70% (P ≤ 0.001).ConclusionImplementation of a BPA can improve infectious disease screening for patients with ARD who are started on b/tsDMARDs and has potential to improve patient safety.
Combining PEDOT:PSS Polymer Coating with Metallic 3D Nanowires Electrodes to Achieve High Electrochemical Performances for Neuronal Interfacing Applications
Authors: Muguet Ines , Maziz Ali , Mathieu Fabrice , Mazenq Laurent , Larrieu Guilhem 
Published: June 2023

AbstractThis study presents a novel approach to improve the performance of microelectrode arrays (MEAs) used for electrophysiological studies of neuronal networks. The integration of 3D nanowires (NWs) with MEAs increases the surface‐to‐volume ratio, which enables subcellular interactions and high‐resolution neuronal signal recording. However, these devices suffer from high initial interface impedance and limited charge transfer capacity due to their small effective area. To overcome these limitations, the integration of conductive polymer coatings, poly(3,4‐ethylenedioxythiophene)‐poly(styrenesulfonate) (PEDOT:PSS) is investigated as a mean of improving the charge transfer capacity and biocompatibility of MEAs. The study combines platinum silicide‐based metallic 3D nanowires electrodes with electrodeposited PEDOT:PSS coatings to deposit ultra‐thin (<50 nm) layers of conductive polymer onto metallic electrodes with very high selectivity. The polymer‐coated electrodes were fully characterized electrochemically and morphologically to establish a direct relationship between synthesis conditions, morphology, and conductive features. Results show that PEDOT‐coated electrodes exhibit thickness‐dependent improved stimulation and recording performances, offering new perspectives for neuronal interfacing with optimal cell engulfment to enable the study of neuronal activity with acute spatial and signal resolution at the sub‐cellular level.
Sorting, Characterization, Environmentally Friendly Recycling, and Reuse of Components from End‐of‐Life 18650 Li Ion Batteries
Authors: Cattaneo Pietro , Callegari Daniele , Merli Daniele , Tealdi Cristina , Vadivel Dhanalakshmi , Milanese Chiara , Kapelyushko Valeriy , D'Aprile Fiorenza , Quartarone Eliana 
Published: June 2023

AbstractThe rapid growth in demand for lithium‐ion batteries (LIBs) is posing challenges in the management of end‐of‐life (EoL) systems and the supply of critical raw materials (CRMs), especially lithium, cobalt, and nickel. Such challenges can be addressed by collecting and recycling spent LIBs through economically and environmentally sustainable processes and by enabling the transition to a circular economy vision based on the use of secondary raw materials. These processes involve not only the metallurgic approaches to recover the critical metals, but also the pretreatment approaches that are crucial to enhance the recovery efficiency of other valuable materials (e.g., graphite, fluorinated compounds, binders, electrolyte). Herein, pretreatment processes ranging from the disassembling, opening, and sorting to the component separation, collection, and recovery, are described for the EoL 18650‐type commercial LIB. A closed loop of eco‐friendly recycling to fully recover the composite cathode, i.e., the cathode active material (CAM), the fluorinated binder, and the conductive carbon, as well as the separator, is presented. The recovery approach is based on green solvents and is designed to limit water consumption. The recovered materials are used to assemble a new cell, and the electrochemical characterization is used to evaluate the effective feasibility of the whole recycling process.
Adaptive Robust Control for Uncertain Underactuated Mechanical Systems
Authors: Zhang Jiale , Jiao Shengjie , Yi Xiaogang , Wu Chenguang 
Published: June 2023

AbstractAn adaptive robust control method is proposed for underactuated mechanical systems (UMSs) with uncertainty in the paper. UMSs are supposed to follow prescribed constraints when there is uncertainty. Inspired by the Udwadia–Kalaba modeling approach, those constraints are considered as control objectives realized by robust control. The uncertainty considered in UMSs is characterized as time‐varying and bounded, albeit with unknown bounds. In order to estimate the boundary information, a novel adaptive law is developed. By using the Lyapunov function, the UMS is guaranteed to be uniform boundedness and uniform ultimate boundedness. The simulation is validated by using a two‐wheeled bicycle as an example, compared to conventional PD control methods. The simulation comparison results show that the proposed control method exhibits notable characteristics, including fast transient response, small overshoots, and good robust performance.
Identification of Histone Lysine Acetoacetylation as a Dynamic Post‐Translational Modification Regulated by HBO1
Authors: Gao Yan , Sheng Xinlei , Tan Doudou , Kim SunJoo , Choi Soyoung , Paudel Sanjita , Lee Taeho , Yan Cong , Tan Minjia , Kim Kyu Min , Cho Sam Seok , Ki Sung Hwan , Huang He , Zhao Yingming , Lee Sangkyu 
Published: June 2023

AbstractKetone bodies have long been known as a group of lipid‐derived alternative energy sources during glucose shortages. Nevertheless, the molecular mechanisms underlying their non‐metabolic functions remain largely elusive. This study identified acetoacetate as the precursor for lysine acetoacetylation (Kacac), a previously uncharacterized and evolutionarily conserved histone post‐translational modification. This protein modification is comprehensively validated using chemical and biochemical approaches, including HPLC co‐elution and MS/MS analysis using synthetic peptides, Western blot, and isotopic labeling. Histone Kacac can be dynamically regulated by acetoacetate concentration, possibly via acetoacetyl‐CoA. Biochemical studies show that HBO1, traditionally known as an acetyltransferase, can also serve as an acetoacetyltransferase. In addition, 33 Kacac sites are identified on mammalian histones, depicting the landscape of histone Kacac marks across species and organs. In summary, this study thus discovers a physiologically relevant and enzymatically regulated histone mark that sheds light on the non‐metabolic functions of ketone bodies.
Efficient Mining of Anticancer Peptides from Gut Metagenome
Authors: Ma Yue , Liu Xiaolin , Zhang Xuan , Yu Ying , Li Yujing , Song Moshi , Wang Jun 
Published: June 2023

AbstractThe gut microbiome plays a crucial role in modulating host health and disease. It serves as a vast reservoir of functional molecules that hold great potential for clinical applications. One specific area of interest is identifying anticancer peptides (ACPs) for innovative cancer therapies. However, ACPs discovery is hindered by a heavy reliance on experimental methodologies. To overcome this limitation, we here employed a novel approach by leveraging the overlap between ACPs and antimicrobial peptides (AMPs). By combining well‐established AMP prediction methods with mining techniques in metagenomic cohorts, a total of 40 potential ACPs is identified. Out of the identified ACPs, 39 demonstrated inhibitory effects against at least one cancer cell line, exhibiting significant differences from known ACPs. Moreover, the therapeutic potential of the two most promising peptides in a mouse xenograft cancer model is evaluated. Encouragingly, the peptides exhibit effective tumor inhibition without any detectable toxic effects. Interestingly, both peptides display uncommon secondary structures, highlighting its distinctive characteristics. This findings highlight the efficacy of the multi‐center mining approach, which effectively uncovers novel ACPs from the gut microbiome. This approach has significant implications for expanding treatment options not only for CRC, but also for other cancer types.
HaptGlove—Untethered Pneumatic Glove for Multimode Haptic Feedback in Reality–Virtuality Continuum
Authors: Qi Jiaming , Gao Feng , Sun Guanghui , Yeo Joo Chuan , Lim Chwee Teck 
Published: June 2023

AbstractNovel haptics technologies are urgently needed to bridge the gap between entirely physical world and fully digital environment to render a more realistic and immersive human–computer interaction. Current virtual reality (VR) haptic gloves either deliver limited haptic feedback or are bulky and heavy. The authors develop a haptic glove or HaptGlove, an untethered and lightweight pneumatic glove, that allows users to “physically” interact in a VR environment and enables both kinesthetic and cutaneous sensations naturally and realistically. Integrated with five pairs of haptic feedback modules and fiber sensors, HaptGlove provides variable stiffness force feedback and fingertip force and vibration feedback, allowing users to touch, press, grasp, squeeze, and pull various virtual objects and feel the dynamic haptic changes. Significant improvements in VR realism and immersion are observed in a user study with participants achieving 78.9% accuracy in sorting six virtual balls of different stiffnesses. Importantly, HaptGlove facilitates VR training, education, entertainment, and socialization in a reality–virtuality continuum.
Long‐Read Sequencing Reveals Extensive DNA Methylations in Human Gut Phagenome Contributed by Prevalently Phage‐Encoded Methyltransferases
Authors: Sun Chuqing , Chen Jingchao , Jin Menglu , Zhao Xueyang , Li Yun , Dong Yanqi , Gao Na , Liu Zhi , Bork Peer , Zhao Xing‐Ming , Chen Wei‐Hua 
Published: June 2023

AbstractDNA methylation plays a crucial role in the survival of bacteriophages (phages), yet the understanding of their genome methylation remains limited. In this study, DNA methylation patterns are analyzed in 8848 metagenome‐assembled high‐quality phages from 104 fecal samples using single‐molecule real‐time sequencing. The results demonstrate that 97.60% of gut phages exhibit methylation, with certain factors correlating with methylation densities. Phages with higher methylation densities appear to have potential viability advantages. Strikingly, more than one‐third of the phages possess their own DNA methyltransferases (MTases). Increased MTase copies are associated with higher genome methylation densities, specific methylation motifs, and elevated prevalence of certain phage groups. Notably, the majority of these MTases share close homology with those encoded by gut bacteria, suggesting their exchange during phage–bacterium interactions. Furthermore, these MTases can be employed to accurately predict phage–host relationships. Overall, the findings indicate the widespread utilization of DNA methylation by gut DNA phages as an evasion mechanism against host defense systems, with a substantial contribution from phage‐encoded MTases.
InSitu Integrated Fabrication for Multi‐Interface Stabilized and Highly Durable Polyaniline@Graphene Oxide/Polyether Ether Ketone Special Separation Membranes
Authors: Lin Ziyu , Zhong Jundong , Sun Runyin , Wei Yingzhen , Sun Zhonghui , Li Wenying , Chen Liyuan , Sun Yirong , Zhang Haibo , Pang Jinhui , Jiang Zhenhua 
Published: June 2023

AbstractSpecial separation membranes are widely employed for separation and purification purposes under challenging operating conditions due to their low energy consumption, excellent solvent, and corrosion resistance. However, the development of membranes is limited by corrosion‐resistant polymer substrates and precise interfacial separation layers. Herein, polyaniline (PANI) is employed to achieve insitu anchoring of multiple interfaces, resulting in the fabrication of polyaniline@graphene oxide/polyether ether ketone (PANI@GO/PEEK) membranes. Insitu growth of PANI achieves the adequate bonding of the PEEK substrate and GO separation interface, which solves the problem of solution processing of PEEK and the instability of GO layers. By bottom‐up confined polymerization of aniline, it could control the pore size of the separation layer, correct defects, and anchor among polymer, nano‐separation layer, and nano‐sheet. The mechanism of membrane construction within the confined domain and micro‐nano structure modulation is further explored. The membranes demonstrate exceptional stability realizing over 90% rejection in 2 m HCl, NaOH, and high temperatures. Additionally, –membranes exhibit remarkable durability after 240 days immersion and 100 h long‐term operation, which display the methanol flux of 50.2 L m−2 h−1 and 92% rejection of AF (585 g mol−1). This method substantially contributes to special separation membranes by offering a novel strategy.
Low Catalyst Loading Enhances Charge Accumulation for Photoelectrochemical Water Splitting
Authors: Liu Tianying , Li Wei , Wang David Z. , Luo Tongtong , Fei Muchun , Shin Dongyoon , Waegele Matthias M. , Wang Dunwei 
Published: June 2023

AbstractSolar water oxidation is a critical step in artificial photosynthesis. Successful completion of the process requires four holes and releases four protons. It depends on the consecutive accumulation of charges at the active site. While recent research has shown an obvious dependence of the reaction kinetics on the hole concentrations on the surface of heterogeneous (photo)electrodes, little is known about how the catalyst density impacts the reaction rate. Using atomically dispersed Ir catalysts on hematite, we report a study on how the interplay between the catalyst density and the surface hole concentration influences the reaction kinetics. At low photon flux, where surface hole concentrations are low, faster charge transfer was observed on photoelectrodes with low catalyst density compared to high catalyst density; at high photon flux and high applied potentials, where surface hole concentrations are moderate or high, slower surface charge recombination was afforded by low‐density catalysts. The results support that charge transfer between the light absorber and the catalyst is reversible; they reveal the unexpected benefits of low‐density catalyst loading in facilitating forward charge transfer for desired chemical reactions. It is implied that for practical solar water splitting devices, a suitable catalyst loading is important for maximized performance.
Why do we need sex‐balanced studies of autism?
Authors: Nordahl Christine Wu 
Published: June 2023

AbstractMales are diagnosed with autism much more frequently than females, and most research study samples reflect this male predominance. The result is that autistic females are understudied. There is a critical need to increase our understanding of autistic females, both biologically and clinically. The only way to do this is to recruit sex‐balanced cohorts in studies so that similarities and differences between males and females can be evaluated in all autism research studies. The purpose of this commentary is to (1) provide historical context about how females came to be under‐represented in all research, not just in the field of autism and (2) learn from other areas of health and medicine about the potentially dire consequences of not studying both sexes, and (3) draw attention to the need to recruit sex‐balanced cohorts in autism research, particularly in neuroimaging studies.
Automated calibration training for forecasters
Authors: Stone Eric R. , Luu Jason , Costello Cory K. , Somerville Annie H. 
Published: June 2023

AbstractIn two studies, we investigated the effectiveness of an automated form of calibration training via individualized feedback as a means to improve calibration in forecasts. In Experiment 1, this training procedure was tested in a realistic forecasting situation, namely, predicting the outcome of baseball games. Experiment 2 was similar but used a more controlled forecasting task, predicting whether competitors would bust in a modified version of blackjack. In comparison to a control group without training, participants provided with calibration training had reduced confidence levels, which translated into reduced overconfidence and better overall calibration in Experiment 2. The results across both studies suggest that an automated form of individualized performance feedback can reduce the confidence of initially overconfident forecasters.
Disruption of regulatory domains and novel transcripts as disease‐causing mechanisms
Authors: Allou Lila , Mundlos Stefan 
Published: June 2023

AbstractDeletions, duplications, insertions, inversions, and translocations, collectively called structural variations (SVs), affect more base pairs of the genome than any other sequence variant. The recent technological advancements in genome sequencing have enabled the discovery of tens of thousands of SVs per human genome. These SVs primarily affect non‐coding DNA sequences, but the difficulties in interpreting their impact limit our understanding of human disease etiology. The functional annotation of non‐coding DNA sequences and methodologies to characterize their three‐dimensional (3D) organization in the nucleus have greatly expanded our understanding of the basic mechanisms underlying gene regulation, thereby improving the interpretation of SVs for their pathogenic impact. Here, we discuss the various mechanisms by which SVs can result in altered gene regulation and how these mechanisms can result in rare genetic disorders. Beyond changing gene expression, SVs can produce novel gene‐intergenic fusion transcripts at the SV breakpoints.
Inhibitory Effects of Corydalis saxicola Bunting Total Alkaloids on Macrophage Pyroptosis
Authors: Feng Cong , Yong Xiangzhi , Jiang Qiaozhi , Su Zhiheng , Liu Zhenmin , Wu Tiantian , Tao Renchuan 
Published: June 2023

AbstractThis study investigated the effect of Corydalis saxicola Bunting total alkaloids (CSBTA) on pyroptosis in macrophages (Mϕ). In the Mϕ pyroptosis model, an inverted fluorescence microscope was used to assess cell pyroptosis, while a scanning electron microscope was used to observe morphological changes in Mϕ. NLR family pyrin domain‐containing 3 (NLRP3), caspase‐1, and gasdermin D (GSDMD) expression levels were detected by polymerase chain reaction and western blotting, whereas interleukin‐1 (IL‐1) and interleukin‐18 (IL‐18) expression levels were measured by an enzyme‐linked immunosorbent assay. After pretreatment with CSBTA or the caspase‐1 inhibitor, acetyl‐tyrosyl‐valyl‐alanyl‐aspartyl‐chloromethylketone (Ac‐YVAD‐cmk), it was discovered that NLRP3, caspase‐1, and GSDMD expressions were significantly reduced at both the mRNA and protein levels, as were IL‐1 and IL‐18 levels. The inhibitory effects of CSBTA and Ac‐YVAD‐cmk did not differ significantly. These findings indicate that CSBTA blocks Porphyromonas gingivalis‐lipopolysaccharide‐induced Mϕ pyroptosis.
Chemical Synthesis of Peptides and Proteins Bearing Base‐Labile Post‐Translational Modifications: Evolution of the Methods in Four Decades
Authors: Ma Wenjie , Liu Han , Li Xuechen 
Published: June 2023

AbstractThe S‐palmitoylation on Cys residue and O‐acetylation on Ser/Thr residues are two types of base‐labile post‐translational modifications (PTMs) in cells. The lability of these PTMs to bases and nucleophiles makes the peptides/proteins bearing S‐palmitoyl or O‐acetyl groups challenging synthetic targets, which cannot be prepared via the standard Fmoc‐SPPS and native chemical ligation. In this review, we summarized the efforts towards their preparation in the past 40 years, with the focus on the evolution of synthetic methods.
Primary lung adenocarcinoma in transplanted lung in post bilateral lung transplantation
Authors: Mubarak Fathima Shafra , Agrawal Sanjay , Rao Jagan 
Published: June 2023

Key Clinical MessagePrimary lung cancer was reported by a bilateral lung transplant patient without any risk factors. Single lung transplantation should be contemplated instead of double lung transplantation as it has been shown to increase the risk of lung cancers.AbstractThis is a case report of a 37‐year‐old woman, with no history of smoking, who developed adenocarcinoma in her transplanted lung 17 years post transplantation. The development of lung cancer 17 years after transplantation is considered as a rare finding in this case report. According to the Annual Report on Cardiothoracic Organ Transplantation 2019–2020, the NHS Blood and Transplant Data, approximately 156 lung transplants were performed in the UK during 2019–2020. The third most common primary disease group recipient was cystic fibrosis and bronchiectasis. There are several medical complications described in the recipients' post‐lung transplantation, and the increased risk of lung malignancy due to immunosuppression is well‐established in comparison to the general population. Most cancers, however, develop in the native lung following a single lung transplant. Several cases of lymphoproliferative malignancies in the transplanted lung have been reported following bilateral lung transplantation. This is a case report of a 37‐year‐old woman with no history of smoking who developed adenocarcinoma in her transplanted lung 17 years later. This patient underwent lobectomy via thoracotomy and was discharged home in good condition. Only a few cases of patients developing primary lung cancer in the transplanted lung with no recipient risk factor have been reported in the literature to date. The development of lung cancer 17 years after transplantation was a rare finding in this case report.
Beyond entrepreneurial failure: Framing failure in the context of family business succession
Authors: Yezza Hedi , Chabaud Didier 
Published: June 2023

AbstractEntrepreneurial failure in the business creation context has received increasing attention over the past 2 decades. However, family business succession failure remains largely unexplored. Accordingly, the aim of this research is to understand the meaning of failure during family succession by proposing a typology of failure adapted to this context. A longitudinal qualitative study was conducted over six years with seven Tunisian family businesses, involving 54 interviews, mainly with their incumbent and successor. The main results highlight diverse perceptions of failure among successors, incumbents and other stakeholders. The findings also demonstrate that the family dimension must be considered to better understand business succession failure.