bluuebunny/crossref_metadata_2025_split huggingface dataset:
The impact of chemical pollution on the European eel (Anguilla anguilla) from a Mediterranean hypersaline coastal lagoon
Authors: Martínez-Gómez Concepción , Fernández Beatriz , Barcala Elena , García-Aparicio Víctor , Jumilla Esther , Gea-Pacheco Ángel , León Víctor Manuel 
Published: June 2023

Abstract
The European eel (Anguilla anguilla) is a critically endangered species. The impact of environmental contamination on this species has been highlighted as contributing to the decline in recruitment. The Mar Menor hypersaline coastal lagoon (SE Spain) is one of the most productive fisheries of European eel in Europe, making it a critical habitat for species conservation. The present study aimed to provide an initial overview of the impact of organic chemical contaminants on the European eel and the potential sublethal effects of chemical pollution on pre-migrating eels in this hypersaline habitat. We investigated muscle bioaccumulation of main persistent and hazardous organic contaminants (including some current-use pesticides) and genotoxicity, neurotoxicity, and xenobiotic detoxification system responses. The findings show that lagoon eels were exposed to high levels of legacy organochlorine contaminants, recently banned pesticides (chlorpyrifos), and some emerging chemicals. Some individuals surpassed the maximum levels of CBs authorized by the European Commission for human consumption. In this species, residuals of chlorpyrifos, pendimethalin, and chlorthal dimethyl have been reported for the first time. This field study provides relevant data to stock management and human health consumption and provides the first biomarker responses in European eel under permanent hypersaline conditions. Furthermore, the high frequency of micronuclei in peripheral erythrocytes of lagoon eels indicates sublethal genotoxic effects on the organism. Overall, the European eels growing and maturing in the Mar Menor lagoon are exposed to toxic and carcinogenic chemicals. The lack of seafood safety regulations for human consumption for some legacy chemicals that were measured in high concentrations in our study requires special action. Further biomonitoring and research are recommended to protect the animal, public, and environmental health.
Empirical studies on the impact of booking status on customers’ choice behavior in online appointment systems
Authors: Kaluza Isabel , Voigt Guido , Paetz Friederike 
Published: June 2023

AbstractWe consider customers' choice behavior in online appointment systems. In three online experiments, we investigate whether and to what extent customers are impacted by the number of available slots by asking subjects to choose between two providers of several service areas with different occupancy rates. In line with previous literature, we find some evidence that customers infer quality from a utilized system compared to an empty schedule; that is, any demand (very few booked appointments) is preferred to no demand (empty booking schedule). A too-small offer set, in contrast, shows congestion and leads to an opposed scarcity effect because customers expect the provider to be in a rush, the waiting room to be crowded, and further value flexibility in the choice of the appointment time. We contribute to the literature by analyzing the interaction of the two nonlinear effects and further find that the presence of the quality-inference effect depends on the type of service. For a standardized service, we find no evidence of the quality-inference effect. For nonstandardized services, however, we find an inverse U-shaped preference in the number of offered slots, showing that customers prefer a medium utilization of the service provider. We find that this is a robust representation of customers' preferences, even if other quality signals, such as star ratings and prices, are available.
Advances in Osteoporosis Therapy: Focus on Osteoanabolic Agents, Secondary Fracture Prevention, and Perioperative Bone Health
Authors: Kostenuik Paul J. , Binkley Neil , Anderson Paul A. 
Published: June 2023

Abstract
Purpose of Review
This review summarizes recently published data and other developments around osteoanabolic osteoporosis therapies in patients with very high fracture risk, including those undergoing bone-related surgery.

Recent Findings
Two osteoanabolic agents, abaloparatide and romosozumab, were recently approved for treatment of patients with osteoporosis at high fracture risk. These agents, along with teriparatide, are valuable for primary and secondary fracture prevention. Orthopedic surgeons are well positioned to facilitate secondary fracture prevention via referrals to fracture liaison services or other bone health specialist colleagues. This review aims to help surgeons understand how to identify patients with sufficiently high fracture risk to warrant consideration of osteoanabolic therapy. Recent evidence around the perioperative use and potential benefits of osteoanabolic agents in fracture healing and other orthopedic settings (e.g., spinal fusion and arthroplasty) in individuals with osteoporosis is also discussed.

Summary
Osteoanabolic agents should be considered for patients with osteoporosis at very high fracture risk, including those with prior osteoporotic fractures and those with poor bone health who are undergoing bone-related surgery.
Ionomic Variation Among Tissues in Fallow Deer (Dama dama) by Sex and Age
Authors: Wenting Elke , Siepel Henk , Christerus Melanie , Jansen Patrick A. 
Published: June 2023

Abstract(1) In mammals, the mineral nutrient and trace elemental composition of the body - the ionome - differs among individuals. It has been hypothesized that these differences may be related to age and sex, both for ecotoxic and essential elements. (2) We investigated whether and how intraspecific ionomic variation is related to age and sex in Fallow deer (Dama dama). We tested the predictions that concentrations of ecotoxic elements increase with age, that ionomic variation is lower among young individuals than among older individuals, and that reproductive females (does) have the lowest concentrations of essential elements. (3) Culled animals of different sex and age were obtained from a single protected area. The animals were dissected to collect 13 tissues, and concentrations of 22 different elements were measured in a sample of each tissue. (4) We described substantial ionomic variation between individuals. Some of this variation was related to age and sex, as predicted. Based on the limited existing knowledge on chemical element allocation and metabolism in the body, sex-related differences were more difficult to interpret than age-related differences. Since reference values are absent, we could not judge about the consequences of the elemental values that we found. (5) More extensive ionomic surveys, based on a wide range of elements and tissues, are needed to enlarge the understanding of within-species ionomic variation and potential biological, ecological, and metabolic consequences.
Capicua (CIC) mutations in gliomas in association with MAPK activation for exposing a potential therapeutic target
Authors: Darabi Sourat , Xiu Joanne , Samec Timothy , Kesari Santosh , Carrillo Jose , Aulakh Sonikpreet , Walsh Kyle M. , Sengupta Soma , Sumrall Ashley , Spetzler David , Glantz Michael , Demeure Michael J. 
Published: June 2023

AbstractGliomas are the most prevalent neurological cancer in the USA and care modalities are not able to effectively combat these aggressive malignancies. Identifying new, more effective treatments require a deep understanding of the complex genetic variations and relevant pathway associations behind these cancers. Drawing connections between gene mutations with a responsive genetic target can help drive therapy selections to enhance patient survival. We have performed extensive molecular profiling of the Capicua gene (CIC), a tumor and transcriptional suppressor gene, and its mutation prevalence in reference to MAPK activation within clinical glioma tissue. CIC mutations occur far more frequently in oligodendroglioma (52.1%) than in low-grade astrocytoma or glioblastoma. CIC-associated mutations were observed across all glioma subtypes, and MAPK-associated mutations were most prevalent in CIC wild-type tissue regardless of the glioma subtype. MAPK activation, however, was enhanced in CIC-mutated oligodendroglioma. The totality of our observations reported supports the use of CIC as a relevant genetic marker for MAPK activation. Identification of CIC mutations, or lack thereof, can assist in selecting, implementing, and developing MEK/MAPK-inhibitory trials to improve patient outcomes potentially.
Development of generalizable automatic sleep staging using heart rate and movement based on large databases
Authors: Lee Joonnyong , Kim Hee Chan , Lee Yu Jin , Lee Saram 
Published: June 2023

Abstract
Purpose
With the advancement of deep neural networks in biosignals processing, the performance of automatic sleep staging algorithms has improved significantly. However, sleep staging using only non-electroencephalogram features has not been as successful, especially following the current American Association of Sleep Medicine (AASM) standards. This study presents a fine-tuning based approach to widely generalizable automatic sleep staging using heart rate and movement features trained and validated on large databases of polysomnography.

Methods
A deep neural network is used to predict sleep stages using heart rate and movement features. The model is optimized on a dataset of 8731 nights of polysomnography recordings labeled using the Rechtschaffen & Kales scoring system, and fine-tuned to a smaller dataset of 1641 AASM-labeled recordings. The model prior to and after fine-tuning is validated on two AASM-labeled external datasets totaling 1183 recordings. In order to measure the performance of the model, the output of the optimized model is compared to reference expert-labeled sleep stages using accuracy and Cohen’s κ as key metrics.

Results
The fine-tuned model showed accuracy of 76.6% with Cohen’s κ of 0.606 in one of the external validation datasets, outperforming a previously reported result, and showed accuracy of 81.0% with Cohen’s κ of 0.673 in another external validation dataset.

Conclusion
These results indicate that the proposed model is generalizable and effective in predicting sleep stages using features which can be extracted from non-contact sleep monitors. This holds valuable implications for future development of home sleep evaluation systems.
Therapeutic effects of isoquercetin on ovariectomy-induced osteoporosis in mice
Authors: Wu Mengjing , Qin Mengyu , Wang Xian 
Published: June 2023

AbstractBone marrow mesenchymal stem cells (BMSCs) are non-hematopoietic multipotent stem cells capable of differentiating into mature cells. Isoquercetin, an extract from natural sources, has shown promise as a potential treatment for osteoporosis. To investigate the therapeutic effects of isoquercetin on osteoporosis, bone marrow mesenchymal stem cells (BMSCs) were cultured in vitro, and osteogenesis or adipogenesis was induced in the presence of isoquercetin for 14 days. We evaluated cell viability, osteogenic and adipogenic differentiation, as well as mRNA expression levels of Runx2, Alpl, and OCN in osteoblasts, and mRNA expression levels of Pparγ, Fabp4, and Cebpα in adipocytes. The results showed that isoquercetin dose-dependently increased cell viability and promoted osteogenic differentiation, as evidenced by Alizarin Red and alkaline phosphatase staining and mRNA expression levels of Runx2, Alpl, and OCN in osteoblasts (P < 0.05). In contrast, isoquercetin inhibited adipogenic differentiation and decreased the mRNA expression levels of Pparγ, Fabp4, and Cebpα in adipocytes (P < 0.05). In vivo, isoquercetin treatment increased bone quantity and density in an osteoporosis model mice group, as determined by μCT scanning and immunohistochemistry (P < 0.05). These findings suggest that isoquercetin may have therapeutic potential for osteoporosis by promoting the proliferation and differentiation of BMSCs towards osteoblasts while inhibiting adipogenic differentiation.
Graphical Abstract
Centralisers, complex reflection groups and actions in the Weyl group $$E_6$$
Authors: Niblo Graham A. , Plymen Roger , Wright Nick 
Published: June 2023

AbstractThe compact, connected Lie group $$E_6$$

E
6

admits two forms: simply connected and adjoint type. As we previously established, the Baum–Connes isomorphism relates the two Langlands dual forms, giving a duality between the equivariant K-theory of the Weyl group acting on the corresponding maximal tori. Our study of the $$A_n$$

A
n

case showed that this duality persists at the level of homotopy, not just homology. In this paper we compute the extended quotients of maximal tori for the two forms of $$E_6$$

E
6

, showing that the homotopy equivalences of sectors established in the $$A_n$$

A
n

case also exist here, leading to a conjecture that the homotopy equivalences always exist for Langlands dual pairs. In computing these sectors we show that centralisers in the $$E_6$$

E
6

Weyl group decompose as direct products of reflection groups, generalising Springer’s results for regular elements, and we develop a pairing between the component groups of fixed sets generalising Reeder’s results. As a further application we compute the K-theory of the reduced Iwahori-spherical $$C^*$$

C
∗

-algebra of the p-adic group $$E_6$$

E
6

, which may be of adjoint type or simply connected.
Electromechanical therapy in diabetic foot ulcers patients: A systematic review and meta-analysis
Authors: Rathnayake Ayeshmanthe , Saboo Apoorva , Vangaveti Venkat , Malabu Usman 
Published: June 2023

Abstract
Purpose
Diabetic foot ulcer (DFU) is one of the most devastating and troublesome consequences of diabetes. The current therapies are not always effective because of the complicated aetiology and interactions of local and systemic components in DFU. However, adjunctive therapy (electromechanical therapy) has become the latest modality in recent years, although there is a lack of significant research to support its utilization as a treatment standard. The purpose of this systematic research was to review the literature on the application of electromechanical therapies in the healing of DFUs.

Methods
For this systematic review, we searched PubMed, Medline, EmBase, the Cochrane library, and Google Scholar for the most current research (1990–2022) on electromechanical therapies for DFUs. We used the PICO method (where P is population, I is intervention, C is comparator/control, and O is outcome for our study) to establish research question with the terms [Electromechanical therapy OR Laser therapy OR photo therapy OR Ultrasound therapy OR Shockwave therapy] AND [diabetic foot ulcers OR diabetes] were used as search criteria. Searches were restricted to English language articles only. Whereas, Cochrane handbook of “Systematic Reviews of Interventions” with critical appraisal for medical and health sciences checklist for systematic review was used for risk of bias assessment. There were 39 publications in this study that were deemed to be acceptable. All the suitably selected studies include 1779 patients.

Results
The meta-analysis of 15 included research articles showed the overall effect was significant (P = 0.0002) thus supporting experimental groups have improvement in the DFUs healing in comparison to the control group.

Conclusion
This systematic review and meta-analysis revealed electromechanical treatments are significantly viable options for patients with DFUs. Electromechanical therapy can considerably reduce treatment ineffectiveness, accelerate healing, and minimize the time it takes for complete ulcer healing.
Clinical factors for predicting cardiovascular risk, need for renal replacement therapy, and mortality in patients with non–dialysis-dependent stage 3–5 chronic kidney disease from the Salford Kidney Study
Authors: Filipa Alexandre Ana , Stoelzel Matthias , Kiran Amit , Garcia-Hernandez Alberto , Morga Antonia , Kalra Philip A. 
Published: June 2023

Abstract
Background
Established cardiovascular risk assessment tools lack chronic kidney disease–specific clinical factors and may underestimate cardiovascular risk in non–dialysis-dependent chronic kidney disease (CKD) patients.

Methods
A retrospective analysis of a cohort of patients with stage 3–5 non–dialysis-dependent chronic kidney disease in the Salford Kidney Study (UK, 2002–2016) was performed. Multivariable Cox regression models with backward selection and repeated measures joint models were used to evaluate clinical risk factors associated with cardiovascular events (individual and composite cardiovascular major adverse cardiovascular events), mortality (all-cause and cardiovascular-specific), and need for renal replacement therapy. Models were established using 70% of the cohort and validated on the remaining 30%. Hazard ratios ([95% CIs]) were reported.

Results
Among 2192 patients, mean follow-up was 5.6 years. Cardiovascular major adverse cardiovascular events occurred in 422 (19.3%) patients; predictors included prior history of diabetes (1.39 [1.13–1.71]; P = 0.002) and serum albumin reduction of 5 g/L (1.20 [1.05–1.36]; P = 0.006). All-cause mortality occurred in 740 (33.4%) patients, median time to death was 3.8 years; predictors included reduction of estimated glomerular filtration of 5 mL/min/1.73 m2 (1.05 [1.01–1.08]; P = 0.011) and increase of phosphate of 0.1 mmol/L (1.04 [1.01–1.08]; P = 0.021), whereas a 10 g/L hemoglobin increase was protective (0.90 [0.85–0.95]; P < 0.001). In 394 (18.0%) patients who received renal replacement therapy, median time to event was 2.3 years; predictors included halving of estimated glomerular filtration rate (3.40 [2.65–4.35]; P < 0.001) and antihypertensive use (1.23 [1.12–1.34]; P < 0.001). Increasing age, albumin reduction, and prior history of diabetes or cardiovascular disease were risk factors for all outcomes except renal replacement therapy.

Conclusions
Several chronic kidney disease–specific cardiovascular risk factors were associated with increased mortality and cardiovascular event risk in patients with non–dialysis-dependent chronic kidney disease.

Graphical abstract
The concepts and origins of cell mortality
Authors: Durand Pierre M. , Ramsey Grant 
Published: June 2023

AbstractOrganismal death is foundational to the evolution of life, and many biological concepts such as natural selection and life history strategy are so fashioned only because individuals are mortal. Organisms, irrespective of their organization, are composed of basic functional units—cells—and it is our understanding of cell death that lies at the heart of most general explanatory frameworks for organismal mortality. Cell death can be exogenous, arising from transmissible diseases, predation, or other misfortunes, but there are also endogenous forms of death that are sometimes the result of adaptive evolution. These endogenous forms of death—often labeled programmed cell death, PCD—originated in the earliest cells and are maintained across the tree of life. Here, we consider two problematic issues related to PCD (and cell mortality generally). First, we trace the original discoveries of cell death from the nineteenth century and place current conceptions of PCD in their historical context. Revisions of our understanding of PCD demand a reassessment of its origin. Our second aim is thus to structure the proposed origin explanations of PCD into coherent arguments. In our analysis we argue for the evolutionary concept of PCD and the viral defense-immunity hypothesis for the origin of PCD. We suggest that this framework offers a plausible account of PCD early in the history of life, and also provides an epistemic basis for the future development of a general evolutionary account of mortality.
Pulling through together: social media response trajectories in disaster-stricken communities
Authors: Maldeniya Danaja , De Choudhury Munmun , Garcia David , Romero Daniel M. 
Published: June 2023

AbstractDisasters are extraordinary shocks that disrupt every aspect of the community life. Lives are lost, infrastructure is destroyed, the social fabric is torn apart, and people are left with physical and psychological trauma. In the aftermath of a disaster, communities begin the collective process of healing, grieving losses, repairing damage, and adapting to a new reality. Previous work has suggested the existence of a series of prototypical stages through which such community responses evolve. As social media have become more widely used, affected communities have increasingly adopted them to express, navigate, and build their response due to the greater visibility and speed of interaction that these platforms afford. In this study, we ask if the behavior of disaster-struck communities on social media follows prototypical patterns and what relationship, if any, these patterns may have with those established for offline behavior in previous work. Building on theoretical models of disaster response, we investigate whether, in the short term, community responses on social media in the aftermath of disasters follow a prototypical trajectory. We conduct our analysis using computational methods to model over 200 disaster-stricken U.S. communities. Community responses are measured in a range of domains, including psychological, social, and sense-making, and as multidimensional time series derived from the linguistic markers in tweets from those communities. We find that community responses on Twitter demonstrate similar response patterns across numerous social, aspirational, and physical dynamics. Additionally, through cluster analysis, we demonstrate that a minority of communities are characterized by more intense and enduring emotional coping strategies and sense-making. In this investigation of the relationship between community response and intrinsic properties of disasters, we reveal that the severity of the impact makes the deviant trajectory more likely, while the type and duration of a disaster are not associated with it.
The Spectral Einstein functional and Kastler–Kalau–Walze type theorems
Authors: Yang Yuchen , Wu Tong 
Published: June 2023

AbstractIn this paper, on the basis of defining the spectral Einstein functional associated with the Dirac operator for manifolds with boundary, we prove Kastler–Kalau–Walze type theorem for the spectral Einstein functional associated with the Dirac operator on low-dimensional manifolds with boundary.
The Book of Amos and its Audiences
Authors: Davis Andrew R. 
Published: June 2023

Many studies of the prophetic books assume that a text's addressee and audience are one and the same. Sometimes this is the case, but some prophetic texts feature multiple addressees who cannot be collapsed into a single setting. In this book Andrew R. Davis examines examples of multiple addressees within the book of Amos and argues that they force us to expand our understanding of prophetic audiences. Drawing insight from studies of poetic address in other disciplines, Davis distinguishes between the addressee within the text and the actual audience outside the text. He combines in-depth poetic analysis with historical inquiry and shows the ways that the prophetic discourse of the book of Amos is triangulated among multiple audiences.