bluuebunny/crossref_metadata_2025_split huggingface dataset:
The efficient CO2 fixation catalyzed by K‐doped g‐C3N4 catalyst for synthesizing benzimidazoles at atmospheric pressure
Authors: Tu Xuewei , Zhang Luping , Chen Yutong , Zhu Shouxin , Sun Can , Jin Jiali , Liu Min , Zheng Hui 
Published: May 2023

AbstractThe conversion of CO2 into valuable chemicals to reduce greenhouse gas emissions has received extensive attention. Converting CO2 into pharmaceutical intermediates via graphitic carbon nitride (CN) at atmospheric pressure is a challenge. In this work, a series of novel graphitic carbon nitrides (K‐CN) catalysts with different doping ratios of K were synthesized by post‐treatment of CN with KOH as a dopant under magnetic stirring. Herein, substrates of o‐phenylenediamine with different electron‐donating/withdrawing groups were employed to convert CO2 into high‐value heterocyclic benzimidazoles. The optimal reaction conditions were determined by a single factor optimization approach. A series of benzimidazole derivatives were synthesized with a yield of up to 96% under atmospheric pressure, indicating that the catalyst can efficiently fix CO2. This work not only designs a simple and low‐cost K‐CN catalyst but also provides a new pathway for converting CO2 into valuable benzimidazole derivatives at atmospheric pressure. © 2023 Society of Chemical Industry and John Wiley & Sons, Ltd.
Knowledge and attitude toward human papillomavirus vaccination and associated factors among adolescent school girls in Ambo town, Ethiopia, 2021: A multicenter cross‐sectional study
Authors: Yohannes Ephrem , Beyen Mulugeta W. , Bulto Gizachew A. , Chaka Eshetu E. , Debelo Bikila T. , Erena Maru M. , Tasu Tefera L. 
Published: May 2023

AbstractBackground and AimsThe human papillomavirus (HPV) vaccination has shown considerable promise in lowering the incidence of cervical cancer. However, there are few studies done on knowledge and attitude toward the HPV vaccine, especially in the local setting. Therefore, this study aimed to assess knowledge and attitudes regarding HPV vaccination and related factors among adolescent girls in Ambo town, Ethiopia, in 2021.MethodsA cross‐sectional study was conducted from May 1 to May 30, 2021, at selected schools in Ambo town among 422 adolescent girls. A structured self‐administered questionnaire was used to collect the data. The collected data were coded, and entered into Epi‐Info 7.2.2.6 and exported to STATA 16 for analysis. Descriptive statistics were used to compute summary statistics and proportions. Bivariate and multivariate logistic regression analyses were carried out. The adjusted odds ratio (AOR) along with a 95% confidence interval (CI) was computed to ascertain the association.ResultsCurrent study revealed that 24.9% (95% CI: 20.8–29.0) and 55.6% (95% CI: 50.5–60.4) of respondents had good knowledge and favorable attitude, respectively. The factors like being secondary school students [AOR = 2.01, 95% CI; (1.24, 3.27)], having health workers as a source of information [AOR = 2.13, 95% CI; (1.30, 3.47)], and respondents who have heard about the HPV vaccine at school [AOR = 1.66, 95% CI; (1.02, 2.71)] were significantly associated with respondent's knowledge of HPV vaccine. Moreover, the perceived severity of the diseases [AOR = 2.89, 95% CI; (1.61, 5.21)], and the perceived benefit of the vaccine [AOR = 4.26, 95% CI; (2.43, 7.48)] were factors significantly associated with an attitude of the respondents about the HPV vaccine.ConclusionThere was low knowledge and unfavorable attitude toward HPV vaccination among the school adolescents in the study area. Several seemingly major deterrents of knowledge and attitude toward the HPV vaccination were identified. Therefore, all parties involved should collaborate to improve female students' knowledge and attitudes toward Human Papilloma Virus vaccination.
Technical, ergonomic and cognitive learning methodology in transumbilical single‐port laparoscopic hysterectomy
Authors: Yang Ye , Gu Song , Sang Wei Lin , Liu Wei , Wu Su Fang 
Published: May 2023

AbstractObjectiveWe introduced learning curves on a detailed step protocol and ergonomic aspect to determine key surgical points in transumbilical single‐port laparoscopic hysterectomy (TSPLH) and to popularize both technical and cognitive methodology on laparoendoscopic single‐site surgery (LESS).Materials and MethodsA retrospective analysis of 87 TSPLH procedures was conducted by a single surgeon in three learning stages. Technical, ergonomic, and cognitive steps were introduced, and surgical outcomes were analyzed.ResultsKey production points in TSPLH include developing a clear retroperitoneal space, maintaining appropriate strength and direction with a vaginal manipulator, coagulating the uterine artery, and applying an improved vaginal stump suturing method. Technical factors included instrument domination, hand–eye coordination, and alternating hand functions. Ergonomic techniques focused on shoulder, elbow, arm, wrist, and finger movements, range of motion, muscle power, continuous forces, and flexibility. Improved cognitive factors such as confidence, decision‐making, and communication were also observed.ConclusionsThe study aimed to form methodological education on TSPLH and LESS and benefit more surgeons. The detailed production and key ergonomic points will help guide self‐learning and education.
An improved method for thyroid nodule ultrasound image segmentation based on U2‐Net
Authors: Liu Qiong , Li Yue , Zhai Zi‐Xin , Jia Hai‐Yan , Liu Li‐Ping 
Published: May 2023

AbstractTo achieve accurate segmentation of thyroid nodule ultrasound images, obtain information on the physiological parameters of the lesion area and guide the clinical formulation of individualized treatment plan, an improved network based on the U2‐Net model is proposed in this paper. Thyroid images of 264 patients and 215 healthy volunteers at the First Hospital of Shanxi Medical University from February 2016 to June 2022 are studied, and the digital database thyroid image (DDTI) data set is proposed for data expansion. The experimental results show that the Dice coefficient on the test set was 80.58%, and the mean intersection over union was 81.21%. The improved U2‐Net model has the best segmentation accuracy compared with similar models, realizes the automatic segmentation of thyroid nodules, provides help for manual segmentation, and has good application prospects and clinical value.
Pavlovian learning and conditioned reinforcement
Authors: Madden Gregory J. , Mahmoudi Saba , Brown Katherine 
Published: May 2023

AbstractConditioned reinforcers are widely used in applied behavior analysis. Basic research evidence reveals that Pavlovian learning plays an important role in the acquisition and efficacy of new conditioned‐reinforcer functions. Thus, a better understanding of Pavlovian principles holds the promise of improving the efficacy of conditioned reinforcement in applied research and practice. This paper surveys how (and if) Pavlovian principles are presented in behavior‐analytic textbooks; imprecisions and knowledge gaps within contemporary Pavlovian empirical findings are highlighted. Thereafter, six practical principles of Pavlovian conditioning are presented along with empirical support and knowledge gaps that should be filled by applied and translational behavior‐analytic researchers. Innovative applications of these principles are outlined for research in language acquisition, token reinforcement, and self‐control.
Nonalcoholic Fatty Liver Disease Is Associated With Decreased Bone Mineral Density in Adults: A Systematic Review and Meta‐Analysis
Authors: Su Ying‐Hao , Chien Kuo‐Liong , Yang Shu‐Hua , Chia Wei‐Tso , Chen Jen‐Hau , Chen Yen‐Ching 
Published: May 2023

ABSTRACTThis systematic review and meta‐analysis aimed to investigate the effect of nonalcoholic fatty liver disease (NAFLD) on bone mineral density (BMD) and the risk of osteoporosis and osteoporotic fracture in adults. We searched PubMed, MEDLINE, Embase, CINAHL, Web of Science, Cochrane Library, and Scopus for observational studies published from inception to January 2023 that reported adjusted effect sizes of NAFLD on BMD, osteopenia/osteoporosis, and osteoporotic fracture. The data were synthesized using multilevel and random‐effects models. A total of 19 studies were included; of these, nine (21,294 participants) evaluated the effect of NAFLD on BMD, six (133,319 participants) investigated the risk of osteoporosis, and five (227,901 participants) assessed the risk of osteoporotic fracture. This meta‐analysis showed that NAFLD was associated with decreased BMD (mean difference −0.019 g/cm2, 95% confidence interval [CI] −0.036 to −0.002, I2 = 93%) and increased risks of osteoporosis (adjusted risk ratio [RR] = 1.28, 95% CI 1.08 to 1.52, I2 = 84%) and osteoporotic fractures (adjusted RR = 1.17, 95% CI 1.00 to 1.37, I2 = 67%). Subgroup analyses revealed that NAFLD had a significantly detrimental effect on BMD in men and on the BMD of the femoral neck and total hip. Stratified analyses by ethnicity demonstrated that NAFLD was not associated with BMD, osteoporosis, or osteoporotic fracture in non‐Asian populations. The publication bias of all included studies was low; however, there was considerable heterogeneity among the studies, warranting a careful interpretation of the findings. Overall, our results suggest that NAFLD is associated with decreased BMD and an increased risk of osteoporosis or osteoporotic fractures. Male sex and the BMD of the femoral neck and total hip may be potential risk factors for decreased BMD in adults with NAFLD. Additionally, ethnic disparities were observed between Asian and non‐Asian populations regarding BMD and osteoporotic fractures. © 2023 American Society for Bone and Mineral Research (ASBMR).
Molecular and cellular remodeling of HepG2 cells upon treatment with antitubercular drugs
Authors: Bakshi Shikha , Kaur Maninder , Verma Arpana , Sharma Sadhna 
Published: May 2023

AbstractDrug‐induced liver injury (DILI) is an adverse outcome of the currently used tuberculosis treatment regimen, which results in patient noncompliance, poor treatment outcomes, and the emergence of drug‐resistant tuberculosis. DILI is primarily caused by the toxicity of the drugs and their metabolites, which affect liver cells, biliary epithelial cells, and liver vasculature. However, the precise mechanism behind the cellular damage attributable to first‐line antitubercular drugs (ATDs), as well as the effect of toxicity on the cell survival strategies, is yet to be elucidated. In the current study, HepG2 cells upon treatment with a high concentration of ATDs showed increased perforation within the cell, cuboidal shape, and membrane blebbing as compared with control/untreated cells. It was observed that ATD‐induced toxicity in HepG2 cells leads to altered mitochondrial membrane permeability, which was depicted by the decreased fluorescence intensity of the MitoRed tracker dye at higher drug concentrations. In addition, high doses of ATDs caused cell damage through an increase in reactive oxygen species production in HepG2 cells and a simultaneous reduction in glutathione levels. Further, high dose of isoniazid (50–200 mM), pyrazinamide (50–200 mM), and rifampicin (20–100 µM) causes cell apoptosis and affects cell survival during toxic conditions by decreasing the expression of potent autophagy markers Atg5, Atg7, and LC3B. Thus, ATD‐mediated toxicity contributes to the reduced ability of hepatocytes to tolerate cellular damage caused by altered mitochondrial membrane permeability, increased apoptosis, and decreased autophagy. These findings further emphasize the need to develop adjuvant therapies that can mitigate ATD‐induced toxicity for the effective treatment of tuberculosis.
Circular RNA HIPK3 facilitates ferroptosis in gestational diabetes mellitus by regulating glutathione peroxidase 4 DNA methylation
Authors: Jiang Jinna , Gao Haijie , Zhou Weidong , Cai Hongxia , Liao Liming , Wang Chenhong 
Published: May 2023

AbstractBackgroundGestational diabetes mellitus (GDM) is the most frequently occurring complication during pregnancy, with a high prevalence rate. Ferroptosis, a type of iron‐dependent cell death, is closely associated with GDM nosogenesis. The present study aimed to examine the potential role and mechanism of circHIPK3 in GDM.MethodsPlacental tissues, plasma samples, and HTR‐8/SVneo cells were used. A receiver operating characteristic curve was used to analyze the diagnostic value of circHIPK3 in GDM. Actinomycin D and RnaseR were added to identify circHIPK3 characteristics. The expression of circHIPK3, miR‐1278, and DNA methyltransferase 1 (DNMT1) was assessed using a quantitative reverse transcriptase‐PCR. Cell counting kit‐8 and terminal deoxynucleotidyl transferase dUTP nick end labeling assays and specific kits were employed to assess cell viability, apoptosis, reactive oxygen species (ROS), malondialdehyde, iron, glutathione, and glutathione peroxidase 4 (GPX4) levels.ResultsThe interaction between miR‐1278 and circHIPK3 or DNMT1 was validated via luciferase reporter and RNA pull‐down assays. circHIPK3 expression was found to be high in GDM placental tissues, plasma, and cells, with a high diagnostic value. In high glucose (HG)‐induced HTR‐8/SVneo cells, the inhibition of circHIPK3 provoked cell viability and mitigated cell apoptosis, ROS, and iron levels, but it was rescued through the downregulation of miR‐1278. Mechanism experiments showed that circHIPK3 bound with miR‐1278 targeting DNMT1 in GDM. The elevation in DNMT1 expression abolished the effects of miR‐1278 overexpression on ferroptosis in HG‐cultured HTR‐8/SVneo cells.ConclusionsOverall, circHIPK3 might facilitate ferroptosis via miR‐1278/DNMT1 to regulate GPX4 DNA methylation in HG‐cultured HTR‐8/SVneo cells. CircHIPK3 could be a therapeutic agent for GDM treatment.
Women's participation in decision‐making: Analysis of Bangladesh Demographic and Health Survey data 2017–2018
Authors: Kirkwood Elizabeth K. , Raihana Shahreen , Alam Neeloy Ashraful , Dibley Michael J. 
Published: May 2023

AbstractGender‐based inequities prevent women from partaking in decision‐making. This study is a secondary analysis of the 2017–2018 Bangladesh Demographic and Health Survey (BDHS) data. We examined women's participation in no‐decisions against a set of independent variables to determine the factors associated with not participating in any decisions. The adjusted multivariable logistic regression revealed that women, aged 15–19, with no children, living in larger households, not employed for cash, agreed with at least one reason for husband's beating their wives and were not exposed to print media, were significantly less likely to be involved in any household decisions. This study sheds light on ways to promote women's active participation in decisions and to identify programme‐modifiable indicators for future research.
Liver directed adeno‐associated viral vectors to treat metabolic disease
Authors: Chuecos Marcel A. , Lagor William R. 
Published: May 2023

AbstractThe liver is the metabolic center of the body and an ideal target for gene therapy of inherited metabolic disorders (IMDs). Adeno‐associated viral (AAV) vectors can deliver transgenes to the liver with high efficiency and specificity and a favorable safety profile. Recombinant AAV vectors contain only the transgene cassette, and their payload is converted to non‐integrating circular double‐stranded DNA episomes, which can provide stable expression from months to years. Insights from cellular studies and preclinical animal models have provided valuable information about AAV capsid serotypes with a high liver tropism. These vectors have been applied successfully in the clinic, particularly in trials for hemophilia, resulting in the first approved liver‐directed gene therapy. Lessons from ongoing clinical trials have identified key factors affecting efficacy and safety that were not readily apparent in animal models. Circumventing pre‐existing neutralizing antibodies to the AAV capsid, and mitigating adaptive immune responses to transduced cells are critical to achieving therapeutic benefit. Combining the high efficiency of AAV delivery with genome editing is a promising path to achieve more precise control of gene expression. The primary safety concern for liver gene therapy with AAV continues to be the small risk of tumorigenesis from rare vector integrations. Hepatotoxicity is a key consideration in the safety of neuromuscular gene therapies which are applied at substantially higher doses. The current knowledge base and toolkit for AAV is well developed, and poised to correct some of the most severe IMDs with liver‐directed gene therapy.
Statins in hospitalized COVID‐19 patients: A systematic review and meta‐analysis of randomized controlled trials
Authors: Ren Yajuan , Wang Guizuo , Han Dong 
Published: May 2023

AbstractThis systematic review and meta‐analysis aimed to determine the efficacy of statins in hospitalized patients with coronavirus disease‐2019 (COVID‐19). A systematic search was made of PubMed, Embase, Cochrane Library, and clinicaltrials.gov, without language restrictions. Randomized controlled trials (RCTs) on treatment of COVID‐19 with statins, compared with placebo or standard of care, were reviewed. Seven RCTs (enrolling 1830 participants) met the inclusion criteria. There was no statistically significant difference in all‐cause mortality (risk ratio [RR]: 0.92, 95% confidence interval [CI]: 0.75–1.13), length of hospital stay (weighted mean difference: −0.21 days, 95% CI: −1.01 to 0.59 days), intensive care unit (ICU) admission (RR: 1.84, 95% CI: 0.45–7.55), and mechanical ventilation (RR: 1.09, 95% CI: 0.70–1.70) between the two groups. Statins failed to reduce mortality, ICU admission, mechanical ventilation, and length of stay in hospitalized patients with COVID‐19. Statins probably should not be used routinely in COVID‐19 patients.
Histone mRNA polyadenylation‐mediated inflammation underlies various virus infections and cancers
Authors: Guo Mengbiao , Zhu Jianxi , Hu Zhijie , Wang Qi , Songyang Zhou , Xiong Yuanyan 
Published: May 2023

AbstractThe mechanistic understanding of virus infection and inflammation in many diseases is incomplete. Normally, messenger RNA (mRNA) tails of replication‐dependent histones (RDH) that safeguard naked nuclear DNAs are protected by a specialized stem‐loop instead of polyadenylation. Here, we showed that infection by various RNA viruses (including severe acute respiratory syndrome coronavirus 2) induced aberrant polyadenylation of RDH mRNAs (pARDH) that resulted in inflammation or cellular senescence, based on which we constructed a pARDH inflammation score (pARIS). We further investigated pARIS elevation in various disease conditions, including different types of virus infection, cancer, and cellular senescence. Notably, we found that pARIS was positively correlated with coronavirus disease 2019 severity in specific immune cell types. We also detected a subset of HIV‐1 elite controllers characterized by pARDH “flipping” potentially mediated by HuR. Importantly, pARIS was positively associated with transcription of endogenous retrovirus but negatively associated with most immune cell infiltration in tumors of various cancer types. Finally, we identified and experimentally verified two pARIS regulators, ADAR1 and ZKSCAN1, which was first linked to inflammation. The ZKSCAN1 was known as a transcription factor but instead was shown to regulate pARIS as a novel RNA binding protein. Both regulators were upregulated under most infection and inflammation conditions. In conclusion, we unraveled a potential antiviral mechanism underlying various types of virus infections and cancers.
IRF5‐TNOP3 polymorphisms are associated with elite control of HIV infection: A retrospective study
Authors: Sepúlveda‐Crespo Daniel , Jiménez‐Sousa María A. , Fernández‐Rodíguez Amanda , Muñoz‐Fernández María A. , Jiménez José Luis , Moreno Santiago , Garcia Felipe , Martínez Isidoro , Benito José M. , Rallón Norma , Resino Salvador 
Published: May 2023

AbstractIRF5–TNPO3 polymorphisms have previously been related to immune response, and TNPO3 plays a role in human immunodeficiency virus (HIV)‐1 infection after nuclear import. Therefore, we analyzed the genetic association between IRF5–TNPO3 polymorphisms and the HIV elite control in long‐term nonprogressors (LTNPs). We performed a retrospective cohort study on 183 LTNPs, who were antiretroviral therapy‐naïve with CD4+ ≥ 500 cells/mm3, viral load ≤10 000 copies/mL, and asymptomatic over 10 years after HIV seroconversion. The primary outcome variable was HIV elite control (undetectable viral load in at least 90% of the measurements for at least 1 year). Seven IRF5–TNPO3 polymorphisms were genotyped using Agena Bioscience's MassARRAY platform. We found a significant association between specific IRF5–TNPO3 genotypes and HIV elite control: rs2004640 TT (aOR = 2.05; p = 0.041), rs10954213 AA (aOR = 1.95; p = 0.035), rs2280714 TT (aOR = 2.02; p = 0.031), and rs10279821 CC (aOR = 2.12; p = 0.017). We also found a significant association between IRF5‐TNPO3 haplotype TATC composed of the favorable significant polymorphisms (rs2004640, rs10954213, rs2280714, and rs10279821) and the HIV elite control (aOR = 1.59; p = 0.048). IRF5–TNPO3 rs2004640, rs10954213, rs2280714, and rs10279821 polymorphisms were related to HIV elite control in LTNPs. Our data provide new knowledge about the impact of IRF5–TNPO3 polymorphisms on HIV pathogenesis to understand the phenomenon of natural HIV control.
Static and dynamic adsorption of supramolecular surfactant for oil recovery in high temperature and salinity conditions
Authors: Nieto‐Alvarez David A. , Marín‐León Adlai , Luna‐Rojero Erick E. , Servín‐Nájera Ana G. , Soto‐Castruita Enrique , Cisneros‐Dévora Rodolfo , Cerón‐Camacho Ricardo , Hernández‐Altamirano Raúl , Mena‐Cervantes Violeta Y. , Zamudio‐Rivera Luis S. 
Published: May 2023

AbstractThe adsorption of a supramolecular surfactant formed by the non‐covalent interactions among sodium (E)‐dodec‐2‐ene‐1‐sulfonate, sodium 3‐hydroxydodecane‐1‐sulfonate and 3‐[(3‐dodecanoylamidopropyl)dimethylazaniumyl]‐2‐hydroxypropane‐1‐sulfonate onto limestone under static and dynamic conditions in the presence of seawater and connate water is presented. In the static test at 70°C and atmospheric pressure, the adsorption capacity of the saturated monolayer was 8.65 mg/g for seawater and 4.05 mg/g for connate water. This behavior was associated with the increase of the critical micellar concentration from 217 ppm in seawater to 672 ppm in connate water, which is related to the capacity of the supramolecular surfactant to form complexes at high salinity and hardness. The dynamic experiment was carried out at 130°C with a pressure of 16,500 kPa, a flow rate of 90 mL/h and a dose of 2000 ppm of the surfactant. An adsorption of 0.230 mg/g was found in seawater, while a value of 0.109 mg/g was obtained in connate water, consistent with the static experiment. In addition, spontaneous imbibition experiments for connate and connate water with 2000 ppm of surfactant showed an increase in the oil recovery factor from 3% to 17% in the presence of surfactant at 100°C. It was observed that the rock changed from oil‐wettable to water‐wettable, indicating that the supramolecular complex retains its capability to form the monolayer on the rock surface. In contrast to the ionic and cationic conventional surfactants, the experimental evidence indicates that the surfactant preserves its properties under the extreme conditions found in mature Mexican carbonate reservoirs.