bluuebunny/crossref_metadata_2025_split huggingface dataset:
UPAYA PENINGKATAN PERSEPSI PERAWATAN KAKI PASIEN DIABETES MELITUS TIPE 2: ACTION RESEARCH DI KLINIK PRATAMA 24 JAM FIRDAUS UMY
Authors: Suib Suib 
Published: February 2023

Background: Diabetic Mellitus (DM) patient rarely to obtain health education related foot care from the health services, therefore patients perceived that DM foot care is unnecessary. This study was aimed to enhance the patientâ€™s perception on Diabetic foot care in DM type 2 in the Firdaus Primary Clinic Muhammadiyah University of Yogyakarta (UMY).Â Methods: Qualitative study with action research design which composed three cycles was applied to frame this study. Six of DM type 2 clients in the Firdaus Primary Clinic Muhammadiyah University of Yogyakarta (UMY) were selected to participate as respondents in this research.Results: Before received intervention, DM patients were assumed that foot care was not being required and has not performed special foot care yet. This might happen because insufficient information related the diabetic foot care.DM type 2 client have perceived that diabetic foot care was the same treatment like healthy people have. After received intervention and perform the foot care at the first cycle, the DM patients were perceived that foot care is important. They felt some foot disorder such as numb, aches and pain were decreased after performing the foot care at the second stage. Moreover, at the third cycle evaluationâ€™s patients still conducting the foot care. Furthermore, diabetic foot care has become daily activity because they felt the advantages.Conclusion: Based on the Focus Group Discussion (FGD) analysis and interview with the DM type 2 patients found that the diabetic foot care training could improve perception related the importance and beneficence of foot care so that patients keep performing the foot care.
Biomechanical evaluation of custom-made short implants with wing retention applied in severe atrophic maxillary posterior region restoration: A three-dimensional finite element analysis
Authors: Yang Zhen , Zhang Jingran , Xu Zexian , Liu Xiaoqiang , Yang Jianjun , Tan Jianguo 
Published: February 2023

Severe bone atrophy in the maxillary posterior region poses a big challenge to implant restoration. Digitally designed and customized short implants with wing retention provide a safer and minimally invasive implant restoration scheme in such circumstances. Small titanium wings are integrated with the short implant supporting the prosthesis. Using digital designing and processing technology, the wings fixed by titanium screws can be flexibly designed, providing the main fixation. The design of the wings will influence the stress distribution and implant stability. This study analyzes the position, structure, and spread area of the wings fixture scientifically by means of three-dimensional finite element analysis. The design of the wings is set to linear, triangular, and planar styles. Under the simulated vertical and oblique occlusal forces, the implant displacement and stress between the implant and the bone surface are analyzed at different bone heights of 1 mm, 2 mm, and 3 mm. The finite element results show that the planar form can better disperse the stress. By adjusting the cusp slope to reduce the influence of lateral force, short implants with planar wing fixtures can be used safely even if the residual bone height is only 1 mm. The results of the study provide a scientific basis for the clinical application of this new customized implant.
Gut microbiota in patients with COVID-19 and type 2 diabetes: A culture-based method
Authors: Petakh Pavlo , Kobyliak Nazarii , Kamyshnyi Aleksandr 
Published: February 2023

BackgroundThe global pandemic of coronavirus disease 2019 (COVID-19) continues to affect people around the world, with one of the most frequent comorbidities being Type 2 Diabetes (T2D). Studies have suggested a link between disbalances in gut microbiota and these diseases, as well as with COVID-19, potentially due to inflammatory dysfunction. This study aims to analyze the changes in gut microbiota in COVID-19 patients with T2D using a culture-based method.MethodsThe stool samples were taken from 128 patients with confirmed COVID-19. Changes in the composition of gut microbiota were analyzed by culture-based method. The study used chi-squared and t-test to find significant differences in gut bacteria between samples and non-parametric correlation analysis to examine relationship between gut bacteria abundance, C‐reactive protein (CRP) levels and length of stay (LoS) in COVID-19 patients without T2D.ResultsThe gut microbiota of T2D patients with COVID-19 showed increased Clostridium spp., Candida spp., and decreased Bifidobacterium spp., Lactobacillus spp. Metformin-treated patients with T2D and COVID-19 without antibiotic treatment showed increased Bacteroides spp., Lactobacillus spp., and decreased Enterococcus, Clostridium compared to the same group with antibiotic treatment. The study also found a positive correlation between the abundance of certain gut microbiota genera, such as Klebsiella spp. and Enterococcus spp., and CRP levels and LoS in COVID-19 patients without and with T2D, while the abundance of other genera, such as Bifidobacterium spp. and Lactobacillus spp., was found to have a negative correlation.ConclusionIn conclusion, this study provides important insights into the gut microbiota composition of SARS-CoV-2-infected individuals with T2D and its potential impact on the course of the disease. The findings suggest that certain gut microbiota genera may be associated with increased CRP levels and longer hospital stays. The significance of this study lies in the fact that it highlights the potential role of gut microbiota in the progression of COVID-19 in patients with T2D, and may inform future research and treatment strategies for this patient population. The future impact of this study could include the development of targeted interventions to modulate gut microbiota in order to improve outcomes for COVID-19 patients with T2D.
Detection of immunoreactive proteins of Escherichia coli, Streptococcus uberis, and Streptococcus agalactiae isolated from cows with diagnosed mastitis
Authors: Dobrut Anna , Wójcik-Grzybek Dagmara , Młodzińska Agata , Pietras-Ożga Dorota , Michalak Katarzyna , Tabacki Aleksander , Mroczkowska Urszula , Brzychczy-Włoch Monika 
Published: February 2023

IntroductionMastitis is a widespread mammary gland disease of dairy cows that causes severe economic losses to dairy farms. Mastitis can be caused by bacteria, fungi, and algae. The most common species isolated from infected milk are, among others, Streptococcus spp., and Escherichia coli. The aim of our study was protein detection based on both in silico and in vitro methods, which allowed the identification of immunoreactive proteins representative of the following species: Streptococcus uberis, Streptococcus agalactiae, and Escherichia coli.MethodsThe study group included 22 milk samples and 13 serum samples obtained from cows with diagnosed mastitis, whereas the control group constituted 12 milk samples and 12 serum samples isolated from healthy animals. Detection of immunoreactive proteins was done by immunoblotting, while amino acid sequences from investigated proteins were determined by MALDI-TOF. Then, bioinformatic analyses were performed on detected species specific proteins in order to investigate their immunoreactivity.ResultsAs a result, we identified 13 proteins: 3 (molybdenum cofactor biosynthesis protein B, aldehyde reductase YahK, outer membrane protein A) for E. coli, 4 (elongation factor Tu, tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG, GTPase Obg, glyceraldehyde-3-phosphate dehydrogenase) for S. uberis, and 6 (aspartate carbamoyltransferase, elongation factor Tu, 60 kDa chaperonin, elongation factor G, galactose-6-phosphate isomerase subunit LacA, adenosine deaminase) for S. agalactiae, which demonstrated immunoreactivity to antibodies present in serum from cows with diagnosed mastitis.DiscussionDue to the confirmed immunoreactivity, specificity and localization in the bacterial cell, these proteins can be considered considered potential targets in innovative rapid immunodiagnostic assays for bovine mastitis, however due to the limited number of examined samples, further examination is needed.
Dietary precursors and cardiovascular disease: A Mendelian randomization study
Authors: Jing Wangwei , Huang Shushi , Xiang Pingping , Huang Jiniu , Yu Hong 
Published: February 2023

BackgroundThe Dietary precursor has been identified as a contributor in the development of cardiovascular disease. However, it is inconsistent if dietary precursors could affect the process of cardiovascular disease.MethodsHere we performed Mendelian randomization (MR) analysis of the data from genome-wide association study of European ancestry to evaluate the independent effects of three dietary precursors on cardiovascular disease (CVD), myocardial infarction (MI), heart failure (HF), atrial fibrillation (AF), and valvular disease (VHD). Inverse variance weighting method was used for the MR estimation. Sensitivity was determined by MR-PRESSO analysis, weighted median analysis, MR-Egger analysis, and Leave-one-out analysis.ResultsWe found that elevated choline level had a causal relationship with VHD [odds ratio (OR) = 1.087, 95% confidence interval (CI), 1.003–1.178, P = 0.041] and MI (OR = 1.250, 95% CI, 1.041–1.501, P = 0.017) by single-variable MR analysis. Furthermore, elevated carnitine level was associated with MI (OR = 5.007, 95% CI, 1.693–14.808, P = 0.004) and HF (OR = 2.176, 95% CI, 1.252–3.780, P = 0.006) risk. In addition, elevated phosphatidylcholine level can increase the risk of MI (OR = 1.197, 95% CI, 1.026–1.397, P = 0.022).ConclusionOur data show that choline increases VHD or MI risk, carnitine increases the risk of MI or HF, and phosphatidylcholine increases HF risk. These findings suggest the possibility that decrease in choline level in circulation may be able to reduce overall VHD or MI risk, reduce in carnitine level could be decrease MI and HF risks as well as decrease in phosphatidylcholine could reduce MI risk.
Paradoxical reduction of plasma lipids and atherosclerosis in mice with adenine-induced chronic kidney disease and hypercholesterolemia
Authors: Padalkar Mugdha V. , Tsivitis Alexandra H. , Gelfman Ylona , Kasiyanyk Mariya , Kaungumpillil Neil , Ma Danyang , Gao Michael , Borges Kelly A. , Dhaliwal Puneet , Nasruddin Saud , Saji Sruthi , Gilani Hina , Schram Eric J. , Singh Mohnish , Plummer Maria M. , Savinova Olga V. 
Published: February 2023

BackgroundAtherosclerotic cardiovascular disease is prevalent among patients with chronic kidney disease (CKD). In this study, we initially aimed to test whether vascular calcification associated with CKD can worsen atherosclerosis. However, a paradoxical finding emerged from attempting to test this hypothesis in a mouse model of adenine-induced CKD.MethodsWe combined adenine-induced CKD and diet-induced atherosclerosis in mice with a mutation in the low-density lipoprotein receptor gene. In the first study, mice were co-treated with 0.2% adenine in a western diet for 8 weeks to induce CKD and atherosclerosis simultaneously. In the second study, mice were pre-treated with adenine in a regular diet for 8 weeks, followed by a western diet for another 8 weeks.ResultsCo-treatment with adenine and a western diet resulted in a reduction of plasma triglycerides and cholesterol, liver lipid contents, and atherosclerosis in co-treated mice when compared with the western-only group, despite a fully penetrant CKD phenotype developed in response to adenine. In the two-step model, renal tubulointerstitial damage and polyuria persisted after the discontinuation of adenine in the adenine-pre-treated mice. The mice, however, had similar plasma triglycerides, cholesterol, liver lipid contents, and aortic root atherosclerosis after being fed a western diet, irrespective of adenine pre-treatment. Unexpectedly, adenine pre-treated mice consumed twice the calories from the diet as those not pre-treated without showing an increase in body weight.ConclusionThe adenine-induced CKD model does not recapitulate accelerated atherosclerosis, limiting its use in pre-clinical studies. The results indicate that excessive adenine intake impacts lipid metabolism.
The association of cell adhesion molecules and selectins (VCAM-1, ICAM-1, E-selectin, L-selectin, and P-selectin) with microvascular complications in patients with type 2 diabetes: A follow-up study
Authors: Siddiqui Khalid , George Teena P. , Mujammami Muhammad , Isnani Arthur , Alfadda Assim A. 
Published: February 2023

ObjectiveChronic hyperglycemia induces pathogenic changes in the vascular endothelium and leads to the development of microvascular complications in patients with type 2 diabetes mellitus. Early identification of markers of diabetes complications may help to minimize the risk of the development and progression of microvascular complications.MethodsThis follow-up study was conducted in type 2 diabetic cohort aged between 30-70 years. Out of 160 eligible participants, 70 of them completed follow-up. Levels of cell adhesion molecules and selectins (VCAM-1, ICAM-1, E-selectin, L-selectin and P-selectin) at baseline and follow-up were measured using Randox Evidence biochip analyzer (UK). Development of microvascular complications (diabetic neuropathy, retinopathy and nephropathy) was evaluated.ResultsDuring the follow-up (2 years, median), 31 (44.3%) developed diabetic neuropathy, 10 (14.3%) developed diabetic retinopathy and, 27 (38.6%) developed diabetic nephropathy. A significant difference in levels of cell adhesion molecules and selectins were found in type 2 diabetic patients with and without microvascular complications. Multiple logistic regression analysis reveals that baseline level of VCAM-1 is significantly associated with microvascular complications; diabetic neuropathy(p=0.028), retinopathy (p=0.007) and nephropathy(p=&lt;0.001). Additionally, levels of P-selectin (p=0.05) and L-selectin (p=0.008) is associated with diabetic nephropathy while retinopathy associated with L-selectin (p=0.005) only.ConclusionCell adhesion molecules and selectins are indicators of microvascular complication among patients with type 2 diabetes (T2D). Association of these markers with the development of microvascular complications may provide additive information for developing strategies for diabetes management and prediction of microvascular complications.
Determinants of poor glycemic control among type 2 diabetes mellitus patients at University of Gondar Comprehensive Specialized Hospital, Northwest Ethiopia: Unmatched case-control study
Authors: Legese Gebrehiwot Lema , Asres Getahun , Alemu Shitaye , Yesuf Tesfaye , Tesfaye Yeabsira Aklilu , Amare Tsegaw 
Published: February 2023

BackgroundPoor glycemic control is one of the most determinant factors for type 2 diabetes-related morbidity and mortality. The proportion of type 2 diabetes mellitus with poor glycemic control remains high. Yet evidences on factors contributing to poor glycemic control remain scarce. The aim of this study is to identify determinants of poor glycemic control among type 2 diabetes mellitus patients at a diabetes mellitus clinic in University of Gondar Comprehensive Specialized Hospital, Northwest Ethiopia.MethodsA hospital-based case-control study was conducted from June to September 2020. Using convenience sampling techniques, a total of 90 cases and 90 controls with type 2 diabetes were recruited. The data were entered into Epidata version 4.6.0.2 and analyzed by Stata version 14. A multivariable logistic regression analysis was performed to assess the association between independent variables and glycemic control. Both 95% CI and p-value&lt;0.05 were used to determine the level and significance, respectively.ResultsThe mean age ( ± standard deviations) for the cases and controls were 57.55± 10.42 and 61.03± 8.93% respectively. The determinants of poor glycemic control were age (Adjusted odd ratio (AOR)= 0.08; 95% CI= 0.02-0.33), inadequate physical exercise (AOR = 5.05; 95% CI = 1.99-11.98), presence of comorbidities (AOR = 5.50; 95% CI = 2.06-14.66), non-adherence to anti-diabetes medications (AOR= 2.76; 95% CI= 1.19-6.40), persistent proteinuria (AOR=4.95; 95% CI=1.83-13.36) and high-density lipoprotein less than 40 mg/dl (AOR=3.08; 95% CI= 1.30-7.31).ConclusionsAge less than 65 years, inadequate physical exercise, presence of comorbidities, non-adherence to anti-diabetes medications, persistent proteinuria, and high-density lipoprotein less than 40 mg/dl were the determinants of poor glycemic control. Therefore, targeted educational and behavioral modification programs on adequate exercise and medication adherence should be routinely practiced. Furthermore, early guideline-based screening and treatment of comorbidities and complications is required to effectively manage diabetes mellitus.
Impacts of urbanization, LULC, LST, and NDVI changes on the static water table with possible solutions and water policy discussions: A case from Islamabad, Pakistan
Authors: Sohail Muhammad Tayyab , Manzoor Zaira , Ehsan Muhsan , Al-Ansari Nadhir , Khan Muhammad Bashir , Shafi Ahsan , Ullah Jar , Hussain Asrar , Raza Danish , Usman Umar , Akbar Sameem , Elbeltagi Ahmed 
Published: February 2023

Rapid urbanization, coupled with land use land cover changes (LULC), has caused stress on freshwater resources around the globe. As in the case of Islamabad, the capital of Pakistan, the population has increased significantly, creating a deficit of natural resources and affecting the environment adversely. Therefore, the purpose of this study is to examine the effects of urbanization and LULC on the decline of the static water table in Islamabad. It also seeks to analyze water policy issues in order to achieve sustainable water resource development. The excessive pumping of the existing groundwater has exceeded the safe limit, which is justified by the constantly growing population. However, the changes in the LULC of the study area have turned many green pastures into barren land. Our research data were obtained from the Capital Development Authority (CDA), Pakistan Meteorological Department (PMD), and Landsat Satellite images. After analyzing PMD and CDA data for the last 20 years (2000–2020), the results were interpreted using Arc GIS. It has been observed that the Normalized Difference Vegetation Index (NDVI) value increases as the Land Surface Temperature (LST) decreases. Therefore, the overall observation is a decreasing trend in Islamabad temperatures due to the increased vegetation in the study area during the period of 2000–2020. It was observed that there has been a considerable drop in water levels due to over-pumping in a few areas. It is primarily associated with the increasing population of the capital in the last 2 decades. This study uses a survey to explore the potential locations for check dams to enhance and recharge the groundwater aquifers in the capital, Islamabad. It suggests catchment areas throughout the Margalla Hills along with different localities, such as Rumli Village, Trail 5, and Shahdara.
Genetic variants in BAT2 are associated with immune responsiveness to influenza vaccination
Authors: Wen Simin , Wei Hejiang , Li Mao , Zhong Shuyi , Cheng Yanhui , Huang Weijuan , Wang Dayan , Shu Yuelong 
Published: February 2023

Background: Influenza is a global public health problem for its detrimental impact on human health. Annual vaccination is the most effective prevention of influenza infection. Identifying host genetic factors associated with the responsiveness to influenza vaccines can provide clues for developing more effective influenza vaccines. In this study, we aimed to explore whether the single nucleotide polymorphisms in BAT2 are associated with the antibody responses to influenza vaccines.Method: A nested case-control study was conducted in this research. 1968 healthy volunteers were enrolled and 1,582 of them from a Chinese Han population were eligible for further research. According to the hemagglutination inhibition titers of subjects against all influenza vaccine strains, a total of 227 low responders and 365 responders were included in the analysis. Six tag single nucleotide polymorphisms in the coding region of BAT2 were selected and genotyped using the MassARRAY technology platform. Univariable and multivariable analyses were conducted to evaluate the relationship between variants and antibody responses to influenza vaccination.Results: Multivariable logistic regression analysis showed that, compared with the BAT2 rs1046089GG genotype, the GA + AA genotype was correlated with decreased risk of low responsiveness to influenza vaccines after adjusting for gender and age (p = 1.12E-03, OR = .562, 95%CI: .398–.795). rs9366785 GA + AA genotype was associated with a higher risk of low responsiveness to influenza vaccination compared with the GG genotype (p = .003, OR = 1.854, 95%CI: 1.229–2.799). The haplotype consisting of BAT2 rs2280801-rs10885-rs1046089-rs2736158-rs1046080-rs9366785 CCAGAG was correlated with a higher level of antibody response to influenza vaccines compared with haplotype CCGGAG (p &lt; .001, OR = .37, 95%CI: .23–.58).Conclusion: Genetic variants in BAT2 were statistically associated with the immune response to influenza vaccination among the Chinese population. Identifying these variants will provide clues for further research on novel broad-spectrum influenza vaccines, and improve the individualized influenza vaccination scheme.
Identification and validation of hub genes and potential drugs involved in osteoarthritis through bioinformatics analysis
Authors: Xu Wenbo , Wang Xuyao , Liu Donghui , Lin Xin , Wang Bo , Xi Chunyang , Kong Pengyu , Yan Jinglong 
Published: February 2023

Purpose: Osteoarthritis (OA) is a common degenerative disease, which still lacks specific therapeutic drugs. Synovitis is one of the most important pathological process in OA. Therefore, we aim to identify and analyze the hub genes and their related networks of OA synovium with bioinformatics tools to provide theoretical basis for potential drugs.Materials and methods: Two datasets were obtained from GEO. DEGs and hub genes of OA synovial tissue were screened through Gene Ontology (GO) annotation, Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment as well as protein—protein interaction (PPI) network analysis. Subsequently, the correlation between expression of hub genes and ferroptosis or pyroptosis was analyzed. CeRNA regulatory network was constructed after predicting the upstream miRNAs and lncRNAs. The validation of hub genes was undertook through RT-qPCR and ELISA. Finally, potential drugs targeting pathways and hub genes were identified, followed by the validation of the effect of two potential drugs on OA.Results: A total of 161 commom DEGs were obtained, of which 8 genes were finally identified as hub genes through GO and KEGG enrichment analysis as well as PPI network analysis. Eight genes related to ferroptosis and pyroptosis respectively were significantly correlated to the expression of hub genes. 24 miRNAs and 69 lncRNAs were identified to construct the ceRNA regulatory network. The validation of EGR1, JUN, MYC, FOSL1, and FOSL2 met the trend of bioinformatics analysis. Etanercept and Iguratimod reduced the secretion of MMP-13 and ADAMTS5 of fibroblast-like synoviocyte.Conclusion: EGR1, JUN, MYC, FOSL1, and FOSL2 were identified as hub genes in the development of OA after series of bioinformatics analysis and validation. Etanercept and Iguratimod seemed to have opportunities to be novel drugs for OA.