bluuebunny/crossref_metadata_2025_split huggingface dataset:
Connect To Care (C2C): protocol for two-site randomized controlled pilot trial to improve outcomes for patients with hazardous drinking and PTSD and/or depression symptoms
Authors: Cucciare Michael A. , Marchant Kathy , Benton Cristy , Hildebrand Deanna , Ghaus Sharfun , Han Xiaotong , Thompson Ronald G. , Timko Christine 
Published: August 2023

Abstract
Background
In studies of the general population and of military veterans, many primary care patients with hazardous drinking and PTSD and/or depression (abbreviated here as HD +) do not initiate or engage with alcohol-related care. To address this gap in care, we identified and will pilot test a promising evidence-based intervention, Connect To Care (C2C). C2C is a strengths-based approach, delivered by a Care Coach by telephone and/or video, with four components: (1) identifying and leveraging patient strengths to facilitate care initiation, (2) collaborative decision-making around a menu of care options, (3) identifying and resolving barriers to care, and (4) monitoring and facilitating progress toward care initiation by, for example, checking on barriers, identifying solutions, and revisiting care options.

Methods/Design
Aim 1 will involve adapting C2C for use in Veterans Affairs’ (VA) primary care. We will use an iterative process that includes focus groups and semi-structured interviews with key stakeholders (patients, primary care providers, and VA national policy leaders). In Aim 2, we will conduct a two-site, pilot randomized controlled trial to determine the feasibility of conducting a larger scale trial to test C2C’s effectiveness, ascertain the acceptability of C2C among primary care patients with HD + , and explore the efficacy of C2C to improve veteran patients’ initiation of and engagement in alcohol care, and their alcohol and mental health (PTSD, depression) outcomes, at 3-month follow-up. We will explore explanatory mechanisms by which C2C is effective.

Discussion
Study findings are likely to have implications for clinical practice to enhance current approaches to linking patients with HD + to alcohol care by applying a practical intervention such as C2C. The results may improve treatment outcomes for people with HD + by drawing on patients’ strengths to problem-solve barriers to care following a process of shared decision-making with a coach. In addition to possibly accelerating the translation of C2C into practice, study findings will also support additional research in terms of a planned effectiveness-implementation hybrid trial, adding to this study’s potential for high impact.
Trial registration: ClinicalTrials.gov Identifier: NCT05023317.
Correlation analysis of quantitative MRI measurements of thigh muscles with histopathology in patients with idiopathic inflammatory myopathy
Authors: Wang Fengdan , Fang Shiyuan , Li Jia , Yuan Ling , Hou Bo , Zhu Jinxia , Jiao Yang , Liu Zhi , Qian Min , Santini Francesco , Wang Qian , Chen Lin , Feng Feng 
Published: August 2023

Abstract
Objectives
To validate the correlation between histopathological findings and quantitative magnetic resonance imaging (qMRI) fat fraction (FF) and water T2 mapping in patients with idiopathic inflammatory myopathy (IIM).

Methods
The study included 13 patients with histopathologically confirmed IIM who underwent dedicated thigh qMRI scanning within 1 month before open muscle biopsy. For the biopsied muscles, FF derived from the iterative decomposition of water and fat with echo asymmetry and least-squares estimation quantitation (IDEAL-IQ) and T2 time from T2 mapping with chemical shift selective fat saturation were measured using a machine learning software. Individual histochemical and immunohistochemical slides were evaluated using a 5-point Likert score. Inter-reader agreement and the correlation between qMRI markers and histopathological scores were analyzed.

Results
Readers showed good to perfect agreement in qMRI measurements and most histopathological scores. FF of the biopsied muscles was positively correlated with the amount of fat in histopathological slides (p = 0.031). Prolonged T2 time was associated with the degree of variation in myofiber size, inflammatory cell infiltration, and amount of connective tissues (p ≤ 0.008 for all).

Conclusions
Using the machine learning-based muscle segmentation method, a positive correlation was confirmed between qMRI biomarkers and histopathological findings of patients with IIM. This finding provides a basis for using qMRI as a non-invasive tool in the diagnostic workflow of IIM.

Relevance statement
By using ML-based muscle segmentation, a correlation between qMRI biomarkers and histopathology was found in patients with IIM: qMRI is a potential non-invasive tool in this clinical setting.

Key points
• Quantitative magnetic resonance imaging measurements using machine learning-based muscle segmentation have good consistency and reproductivity.
• Fat fraction of idiopathic inflammatory myopathy (IIM) correlated with the amount of fat at histopathology.
• Prolonged T2 time was associated with muscle inflammation in IIM.

Graphical Abstract
Integrated structure model-based virtual screening approaches identified anti-cancer agents against prostate cancer by targeting MAOB protein
Authors: Molla Mohammad Habibur Rahman , Asseri Amer H. , Islam Md. Shafiqul 
Published: August 2023

Abstract
Background
Flavin monoamine oxidase gene encodes a protein (MAOB) that forms a part of the flavin monoamine oxidase family in the outer membrane of mitochondria. It plays a role in the tissue metabolism of neuroactive and vasoactive amines as well as the oxidative deamination of xenobiotic and biogenic amines. However, overexpression of the receptor reduced apoptosis in cells, resulting in the progress of prostate sarcoma. Therefore, various kinds of MAOB antagonists are often used to fix an apoptosis mechanism that makes it hard to get rid of cancer from live tissues. Moreover, chemical compounds that have been discovered to be MAOB inhibitors to date exhibit side effects that are causing problems in chemotherapy treatment. The study aims to discover new purchasable compound that induces apoptosis by allowing caspases to operate at their maximum efficiency and is low toxic.

Methods
With the assistance of virtual screening, molecular docking, and molecular dynamics simulation (MD), a structure-based pharmacophore model of the protein active site cavity was made. Twenty hits were found, and then a molecular docking strategy was used to choose four molecules to study in more depth. MD simulations were used to check the stability of the four compounds, and they were all shown to be stable when bound to the target protein.

Results
Four newly discovered compounds, included with ZINC ID Such as ZINC12143050, ZINC08301324, ZINC16743012, and ZINC64165826 with binding scores of − 11.7, − 11.4, − 11.2 and − 11.1 kcal/mol, respectively, may serve as lead compounds for the treatment of prostate cancer associated with MAOB; however, further evaluation through wet lab is needed to determine the compounds effectiveness.

Conclusion
A structure-based model was initially developed, followed by molecular docking, ADMET analysis, and MD simulation. The top four natural compounds identified in the A-to-Z virtual screening process could serve as lead molecules in the fight against prostate cancer.
Using CRISPR/Cas9 to identify genes required for mechanosensory neuron development and function
Authors: Johnson Christopher J. , Kulkarni Akhil , Buxton William J. , Hui Tsz Y. , Kayastha Anusha , Khoja Alwin A. , Leandre Joviane , Mehta Vanshika V. , Ostrowski Logan , Pareizs Erica G. , Scotto Rebecca L. , Vargas Vanesa , Vellingiri Raveena M. , Verzino Giulia , Vohra Rhea , Wakade Saurabh C. , Winkeljohn Veronica M. , Winkeljohn Victoria M. , Rotterman Travis M. , Stolfi Alberto 
Published: August 2023

ABSTRACT
Tunicates are marine, non-vertebrate chordates that comprise the sister group to the vertebrates. Most tunicates have a biphasic lifecycle that alternates between a swimming larva and a sessile adult. Recent advances have shed light on the neural basis for the tunicate larva's ability to sense a proper substrate for settlement and initiate metamorphosis. Work in the highly tractable laboratory model tunicate Ciona robusta suggests that sensory neurons embedded in the anterior papillae transduce mechanosensory stimuli to trigger larval tail retraction and initiate the process of metamorphosis. Here, we take advantage of the low-cost and simplicity of Ciona by using tissue-specific CRISPR/Cas9-mediated mutagenesis to screen for genes potentially involved in mechanosensation and metamorphosis, in the context of an undergraduate ‘capstone’ research course. This small screen revealed at least one gene, Vamp1/2/3, which appears crucial for the ability of the papillae to trigger metamorphosis. We also provide step-by-step protocols and tutorials associated with this course, in the hope that it might be replicated in similar CRISPR-based laboratory courses wherever Ciona are available.
An interaction between OTULIN and SCRIB uncovers roles for linear ubiquitination in planar cell polarity
Authors: Almeida Stephanie M. , Ivantsiv Sofiia , Niibori Rieko , Dunham Wade H. , Green Brooke A. , Zhao Liang , Gingras Anne-Claude , Cordes Sabine P. 
Published: August 2023

ABSTRACT
Planar cell polarity (PCP) plays critical roles in developmental and homeostatic processes. Membrane presentation of PCP complexes containing Van Gogh-like (VANGL) transmembrane proteins is central to PCP and can be directed by the scaffold protein scribble (SCRIB). The role atypical linear ubiquitin (Met1-Ub) chains might play in PCP is unknown. Here, HEK293 cell-based interactomic analyses of the Met1-Ub deubiquitinase OTULIN revealed that OTULIN can interact with SCRIB. Moreover, Met1-Ub chains associated with VANGL2 and PRICKLE1, but not SCRIB, can direct VANGL2 surface presentation. Mouse embryos lacking Otulin showed variable neural tube malformations, including rare open neural tubes, a deficit associated with PCP disruption in mice. In Madin–Darby canine kidney cells, in which the enrichment of VANGL2-GFP proteins at cell-cell contacts represents activated PCP complexes, endogenous OTULIN was recruited to these sites. In the human MDA-MB-231 breast cancer cell model, OTULIN loss caused deficits in Wnt5a-induced filopodia extension and trafficking of transfected HA-VANGL2. Taken together, these findings support a role for linear (de)ubiquitination in PCP signaling. The association of Met1-Ub chains with PCP complex components offers new opportunities for integrating PCP signaling with OTULIN-dependent immune and inflammatory pathways.
An Assessment of Contemporary Methods and Data-Enabled Approaches for Early Cataract Detection
Authors: Muchibwa Chris , Eldaw Muawya H. Sarnoub ,   Mu Mu, Agyeman Michael Opoku 
Published: August 2023

Background: Cataracts are common causes of visual impairment. Preventing blindness requires an early and accurate diagnosis. This review examines current cataract diagnosis strategies, explores data-driven machine learning algorithms for early detection, investigates the use of artificial intelligence (AI) approaches, assesses improvements in cataract detection accuracy, identifies research gaps, and provides recommendations for future studies. Methods: We gathered labelled cataract and non-cataract fundus data from the Kaggle. Scholarly publications were sourced from reliable databases such as ProQuest, IEEE, ELSEVIER, Google Scholar, and PubMed. A detailed literature search with specific terms expanded the scope of this review. We included studies that used cataract and non-cataract fundus eye images from cross-sectional, retrospective, and prospective studies. The quality assessment used the AMSTAR tool, considering factors such as literature search comprehensiveness, study selection criteria, data extraction methodologies, and study validity (Table 1). Results: This study encompassed 130 research publications, focusing on machine learning models and clinical-based diagnostic approaches for early-stage cataract identification. The performance of machine-learning models is influenced by factors such as dataset noise and limited reliable data. Barriers to the successful implementation of AI for cataract diagnosis were identified. Conclusions: This review emphasises the obstacles hindering the broad application of AI in cataract diagnosis. Addressing these findings is vital for developing strategies to overcome these challenges and enhance cataract detection systems. To achieve improved accuracy and efficiency in cataract diagnosis, future research should prioritise efforts to enhance dataset availability and quality, reduce data noise, and refine machine-learning algorithms. Unlocking the full potential of AI and/or machine learning can lead to significant breakthroughs in cataract diagnosis, ultimately resulting in better patient outcomes and reduced visual impairments.
Measurement uncertainty interval in case of a known relationship between precision and mean
Authors: Uhlig Steffen , Colson Bertrand , Gowik Petra 
Published: August 2023

Background: Measurement uncertainty is typically expressed in terms of a symmetric interval y±U, where y denotes the measurement result and U the expanded uncertainty. However, in the case of heteroscedasticity, symmetric uncertainty intervals can be misleading. In this paper, a different approach for the calculation of uncertainty intervals is introduced. Methods: This approach is applicable when a validation study has been conducted with samples with known concentrations. In a first step, test results are obtained at the different known concentration levels. Then, on the basis of precision estimates, a prediction range is calculated. The measurement uncertainty for a given test result can then be obtained by projecting the intersection of the test result with the limits of the prediction range back onto the axis of the known values, now interpreted as representing the measurand. Results: It will be shown how, under certain circumstances, asymmetric uncertainty intervals arise quite naturally and lead to more reliable uncertainty intervals. Conclusions: This article establishes a conceptual framework in which measurement uncertainty can be derived from precision whenever the relationship between the latter and concentration has been characterized. This approach is applicable for different types of distributions. Closed expressions for the limits of the uncertainty interval are provided for the simple case of normally distributed test results and constant relative standard deviation.
The outcome efficacy of the entity risk management requirements of the NIS 2 Directive
Authors: Ferguson Donald David Stewart 
Published: August 2023

AbstractThe NIS 2 Directive (2022/2555) of the European Union (EU) identifies the cybersecurity risk management requirements for essential and important entities in EU member states. The principal question we address is, how effective are the cybersecurity risk management measures of the NIS 2 Directive against cyberattacks on essential and important entities in EU member states? It was observed, through statutory interpretation and cyber kill chain model analysis, that the cybersecurity risk management measures of the NIS 2 Directive may be significantly limited in their effectiveness against cyberattacks on essential and important entities in EU member states. The limited effectiveness is primarily due to the narrow scope of the cybersecurity risk management measures, including the lack of specific measures focused on the reconnaissance phase of a cyberattack.
Systematic review with meta-analysis: Prevalence, risk factors, and challenges for urinary schistosomiasis in children (USC)
Authors: Masdor Noor Azreen , Kandayah Thinakaran , Amsah Norizzati , Othman Rahayu , Hassan Mohd Rohaizat , Rahim Syed Sharizman Syed Abdul , Jeffree Mohammad Saffree , Awang Lukman Khamisah , Hidrus Aizuddin 
Published: August 2023

Background
Schistosomiasis is a parasitic infection that causes significant public health problems in tropical countries. Schistosoma haematobium species are blamable for causing urinary schistosomiasis. The infected person, specifically children, may be carrying the disease. This systematic review aimed to identify the current knowledge of urinary Schistosmiasis in children or USC on its epidemiology, risk factors, and challenges to spread the understanding of controlling the disease and reducing the complications.

Method
In November 2021, a systematic computer-aided literature review was conducted using PubMed, SCOPUS and Web of Science, following the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) criteria. The results were updated in February 2022. We only used papers that have at least the abstract available in English. Relevant articles were screened, duplicates were deleted, eligibility criteria were applied, and studies that met the criteria were reviewed. The keywords Human Schistosoma infections, prevalence, risk factors and challenges were included. The protocol for the review was registered with PROSPERO (registration number CRD42022311609). Pooled prevalence rates were calculated using the programme R version 4.2.1. Heterogeneity was assessed using the I2 statistic and p-value. A narrative approach was used to describe risk factors and challenges. Studies were selected and finalised based on the review question to prioritise. The quality of the included studies was assessed using the Mixed-Method Appraisal Tool (MMAT).

Results
A total of 248 publications met the requirements for inclusion. Fifteen articles were included in this review, with the result showing high heterogeneity. The pooled prevalence of urinary schistosomiasis in children is 4% (95% confidence interval (CI)). Age, poor socioeconomic status, education, exposure to river water, and poor sanitation are the risk factors identified in this review. Challenges are faced due to limitations of clean water, lack of water resources, and poor hygiene.

Conclusion
Modifiable risk factors such as poor knowledge and practices must be addressed immediately. Healthcare providers and schools could accomplish engaging in practical promotional activities. Communicating the intended messages to raise community awareness of urinary schistosomiasis is critical.
Institutional Gaps in Agonistic and Communicative Planning Theories. Critical Implications of the ‘Systemic Turn’ in Deliberative Democracy Theory
Authors: Mäntysalo Raine , Mattila Hanna , Hirvola Aino 
Published: August 2023

The paper critically reviews communicative and agonistic planning theories from the viewpoint of a systemic turn in deliberative democracy theory. While the approach reveals complementarities between the theories, it also argues that each theory is vulnerable to criticism because of an ‘institutional gap’. The theories are found to complement each other in addressing planning conflicts at different dimensions. Communicative planning theory deals with conflicts between different stakeholders’ interests in planning processes. Agonistic planning theory, in turn, concentrates on conflicts from a more ontological dimension, related to the (implicit) conflict between hegemonic and marginalized discourses and related identity-forming processes of inclusion and exclusion in planning policies and governance. The institutional gap of communicative planning theory is argued to reside in its focus on situational deliberation that largely ignores the institutional dimension of rules and norms of democratic conduct. Agonistic pluralism, in turn, does engage with the dimension of democratic institutions, but in an overly critical manner, making it difficult for agonistic planning theory to address the dynamic interplay between institutional reconfiguration and policy stabilization in planning. This is argued to be the institutional gap of agonistic planning theory. The paper calls for further work in the field of planning theory to incorporate a systemic approach to deliberative democracy and thereby tap into the dialectics of institutional and situational dimensions of planning.
A new optimized ZV-ZCS non-isolated high-gain boost converter for renewable energy systems
Authors: Pal Ananta , Saha Shib Sankar 
Published: August 2023

Abstract
This paper proposes a novel zero-voltage zero-current transition (ZV-ZCT) DC/DC high gain coupled inductor based boost converter for applications in renewable energy systems. It employs an auxiliary resonant network to soft-switch transition of semiconductor switches and soft-recovery of power diodes over wide input voltage and output power range of the converter with high efficiency. The auxiliary cell of the proposed converter has negligible impact on the converter’s overall dynamics and it doesn’t contribute any extra current stress to the semiconductor switches compared to classical hard switched boost converters. A thorough theoretical analysis of each operating mode has been presented together with the converter’s steady-state performance. The mathematical equations illustrating the dynamic behaviour of the converter have been used to design the auxiliary cell components. The circulating current loss has been minimized and simultaneously soft-switched operation of the converter is maintained through optimal selection of auxiliary cell components. Additionally, a power loss study and the proposed converter’s advantages over existing traditional converters are discussed. The converter’s soft-switching behaviour is established by hardware testing on a 400 W, 100 kHz prototype model. The theoretically predicted behaviour of the converter has been well corroborated by experimental observations.
Cláusulas escalonadas de solución de controversias: una aproximación a sus principales problemáticas
Authors: Rodríguez Uriol Dario Andrés 
Published: August 2023

Las cláusulas escalonadas de solución de controversias son un tipo de convenio arbitral muy popular hoy en día. Estas cláusulas prevén que, para solucionar las controversias que surjan en torno a una relación contractual, las partes podrán o deberán recurrir a más de un mecanismo de solución de controversias, los cuales serán utilizados de forma progresiva, uno después de otro. Si bien este tipo de pactos pueden presentar ventajas para las partes, también pueden llegar a ser cláusulas problemáticas que, de diseñarse o gestionarse de forma inadecuada, pueden ser fuente de conflictos adicionales a los ya surgidos. Por ello, en el presente artículo, se analizarán las principales problemáticas que surgen en torno a este tipo de acuerdos. Más concretamente, se analizan tres problemáticas en torno a las etapas pre arbitrales: su obligatoriedad, los factores más relevantes a tener en cuenta para su ejecución y cumplimiento, y las consecuencias que su incumplimiento trae para el proceso arbitral.
Ten-Year Follow-Up of a Customized Total Talar Prosthesis for Revision Total Ankle Arthroplasty
Authors: Yano Koichiro , Ikari Katsunori , Okazaki Ken 
Published: August 2023

Case:
A 74-year-old woman with rheumatoid arthritis had undergone total ankle arthroplasty (TAA) for severe ankle joint destruction at our hospital 14 years earlier. Radiographs of the ankle revealed displacement of the tibial component and subsidence of the talar component. Revision TAA was performed using a customized alumina ceramic total talar prosthesis.

Conclusion:
The 10-year outcome of revision TAA with a total talar prosthesis was satisfactory, with no postoperative complications, such as displacement of the tibial component, dislocation of alumina ceramic artificial talus, or progression of degenerative changes in the talonavicular and subtalar joints.