bluuebunny/crossref_metadata_2025_split huggingface dataset:
Metabolomics revealed the anxiety-like behaviors led to liver injury through EGFR/PI3K/AKT/NF-κB pathway in rats
Authors: Liu Binjie , Zhang Shanshan , Sun Lizhu , Huang Lan , Zhang Rong , Liu Zhongqiu , An Lin 
Published: April 2023

Abstract
Psychological distress is associated with increased liver disease mortality. This association highlighted the close relationship between psychological and physical health. Thus, further work to elucidated the underlying mechanism should be carried out. In this experiment, a model of anxious rats was established by compound stress. The HPA axis situation and inflammatory factor changes in the brain of the rats were observed, which in turn were evaluated for behavioral tests and liver function, respectively. The liver metabolic profiles of the rats were characterized by liquid chromatography-mass spectrometry (LC-MS). Differential metabolites were screened by p < 0.05 and VIP > 1. Pathway enrichment analysis was performed on metabolomics data by Ingenuity Pathway Analysis (IPA). Immunofluorescence (IF), immunohistochemistry (IHC) and western blotting, were employed to detect the expression of the screened target epidermal growth factor receptor (EGFR), and verify its potential pathway of mechanism. The results show that we found liver function impairment in anxiety-like rats. Further, 61 differential metabolites in control and anxiety groups were screened by using metabolomics (p < 0.05, VIP > 1). IPA analysis revealed the key target EGFR which was also identified from the HPA axis. We further found that anxiety-like rats may cause liver injury through EFGR/PI3K/AKT/NF-κB pathway, and then lead to the production of inflammatory factors in the liver. This study demonstrated that the activation of HPA axis in anxiety-like rats led to phosphorylation of EGFR, which finally contributed to liver injury through the EGFR/PI3K/AKT/NF-κB pathway. This finding provided novel evidence for the deleterious effects of psychological problems on physical health.
Clicking Through the Clickstream: A Novel Statistical Modeling Approach to Improve Information Usage of Clickstream Data by Ecommerce Entities
Authors: Allenbrand Corban 
Published: April 2023

Abstract

Success or failure of an ecommerce platform is often reduced to its ability to maximize the conversion rate of its visitors. This is commonly regarded as the capacity to induce a purchase from a visitor. Visitors possess individual characteristics, histories, and objectives which complicates the choice of what features of a web system solves the conversion maximization problem. Modern web technology has made clickstream data accessible allowing a complete record of a visitor’s actions on a website to be analyzed. What remains poorly constrained is what parts of the clickstream data are meaningful information and what parts are accidental. In this research clickstream data from an online retailer was examined to provide answers to the previous questions with statistical modeling. A conceptual model was developed from which several hypotheses on the nature of clickstream relationships were posited. A discrete choice logit model was developed which showed that the content of a website, the history of website use, and the exit rate of pages visited had marginal effects on derived utility for the visitor. Exit rate and bounce rate were modeled as beta distributed random variables. It was found that exit rate and its variability for pages visited by visitors was associated with site content, site quality, prior visitor history on the site, and technological preferences of the visitor. Bounce rate was also found to be influenced by the same factors but were in a direction opposite to the registered hypotheses. Most findings supported that clickstream data is open to statistical modeling with interpretable and comprehensible models.
“I literally had no support”: Barriers and facilitators to supporting the psychosocial wellbeing of young people with mental illness in Tasmania, Australia
Authors: Savaglio Melissa , Yap Marie , Smith Toni , Vincent Ash , Skouteris Helen 
Published: April 2023

Abstract
Background There has been limited focus on understanding the barriers and facilitators to meeting the broader psychosocial needs of young people with mental illness, from the perspectives of young people themselves. This knowledge is required to advance the local evidence base and inform service design and development. Therefore, the aim of this qualitative study was to explore young people’s (10–25 years) and carers’ experiences of mental health services, focusing on the barriers and facilitators to services supporting young people’s psychosocial functioning.Method Young people with living experience of mental illness were involved in all stages of this research. Semi-structured interviews were conducted with 32 young people aged 10–25 years with experience of mental illness, and 29 carers (12 were parent-child dyads). Qualitative analysis was guided by the Social-Ecological Framework to identify barriers and facilitators at the individual (young person/carer level), interpersonal, and service/systemic level.Results Young people and carers identified eight barriers and six facilitators across the various levels. Barriers included, at the individual level: (1) the complexity of young people’s psychosocial needs and (2) lack of awareness/knowledge of services available; at the interpersonal level: (3) negative experiences with adults and (4) fragmented communication between services and family; and at the systemic level: (5) lack of services; (6) long waiting periods; (7) limited service accessibility; and (8) the missing middle. Facilitators included, at the individual level: (1) education for carers; the interpersonal level: (2) positive therapeutic relationships and (3) carer advocacy/support; and systemic level: (4) flexible or responsive services, (5) services that address the psychosocial factors; and (6) safe service environments.Conclusions This study identified lived-experience recommendations for public mental health policy and practice, including service design and development. To better enhance their psychosocial functioning, young people and carers want lived-experience workers to provide practical wrap-around support, and they want mental health services that integrate health and social care, and are flexible, responsive and safe. These findings will inform the co-design and development of a new community-based youth psychosocial service to support the psychosocial wellbeing of young people experiencing severe mental illness.
In Situ Assembly of Bioresorbable Organic Bioelectronics in the Brain
Authors: Hjort Martin , Mousa Abdelrazek H. , Bliman David , Shameem Muhammad Anwar , Hellman Karin , Yadav Amit Singh , Ekström Peter , Ek Fredrik , Olsson Roger 
Published: April 2023

Abstract

Bioelectronics has the potential to complement classical therapies in nonchronic treatments, such as immunotherapy and cancer. In addition to functionality, minimally invasive implantation methods and bioresorbable materials are central to nonchronic treatments. The latter is to avoid the need for surgical removal after disease relief. Self-organizing substrate-free organic electrodes have the potential to meet these criteria and the need for seamless integration into dynamic biological systems in a way that is not possible for classical rigid solid-state electronics. We have developed a general approach that is not dependent on specific external or endogenous triggers to assemble bioresorbable electrodes from water-dispersed nanoparticles in the brain. To control precise localization, conductive structures were placed in the targeted area using a capillary thinner than a human hair. An optional auxiliary module grew dendrites from the installed organic electrode to seamlessly embed neurons. This module also modified the conductive structure’s volume properties, such as functionality and biocompatibility while displaying brain matched shear modulus. These soft microdendritic electrodes set off a controlled cellular response in the brain when relaying external stimuli. The materials were well tolerated, and the initial inflammation in the brain caused by the implantation was resolved, showing no tissue damage after bioresorption. These findings initiate the discovery of in vivo assembled, fully integrated, transient organic bioelectronics within cellular structures that can be used for nonchronic treatments.
Production and composition of Extracellular Polymeric Substances (EPS) by a unicellular strain and natural colonies of Microcystis: impact of salinity and nutrient stress
Authors: Reignier Océane , Bormans Myriam , Marchand Laetitia , Sinquin Corinne , Amzil Zouher , Zykwinska Agata , Briand Enora 
Published: April 2023

Abstract
The transfer of toxic cyanobacterial Microcystis blooms from freshwater to estuaries constitutes a serious environmental problem worldwide that is expected to expand in scale and intensity with anthropogenic and climate change. The formation and maintenance of Microcystis in colonial form is conditioned to the presence of Extracellular Polymeric Substances (EPS). In an attempt to better understand how mucilaginous colonial form may evolved under environmental stress condition, we studied and compared the production and the composition of EPS fractions (attached and free) from natural colonies of a Microcystis bloom and from a unicellular M. aeruginosa strain under salinity and nutrient stress (representing a land-sea continuum). Our results highlighted a greater production of EPS from the natural colonies of Microcystis than the unicellular one without or under stress condition. In comparison to the unicellular Microcystis, EPS produced by the colonial form were characterized by high molecular weight polysaccharides which were enriched in uronic acids and hexosamines, notably for the free fraction in response to increased salinities. This complex extracellular matrix gives the cells the ability to aggregate and allows the colonial cyanobacterial population to cope with osmotic shock.
Issues Related to IUCD Discontinuation Rates Among IUCD Users in Kembata Tembaro Zone, Southern Ethiopia
Authors: Yoseph Tessema 
Published: April 2023

Abstract

Background
Intrauterine contraceptive devices (IUCDs) are made of T-shaped plastic and are inserted into women’s uteruses to prevent unwanted pregnancies. Despite the improvement in the availability and utilisation of intrauterine contraceptive devices, discontinuation is becoming a public health concern. A significant proportion of women discontinue the method before its due date, which poses a concern in the health system, and its consequences may lead to the failure of a programme. As a result, the purpose of this study was to investigate the issues related to IUCD discontinuation rates among IUCD users IN twelve months ago in Kembata Tembaro Zone, Southern Ethiopia, in 2022.
Methods
A community-based, cross-sectional study was conducted. 415 women who had inserted an IUD from July 2021 to June 2022 in the Kembata Tembaro Zone were selected using a multistage sampling technique included in the analysis. This study's data collection instruments were pre-tested structured questionnaires. The coded data was entered into Epi Data version 4.6 and exported to SPSS version 25 for analysis. Finally, binary logistic regression analysis was carried out to identify independently associated factors and odds ratios at a 95% confidence interval with a significance level of p-value less than 0.05.
Results
Analysis revealed that 21% (95% CI 16.5–22.8) of women discontinued the use of their intrauterine contraceptive device in the last year. Issues like not being counselled about the intrauterine contraceptive device before insertion [AOR = 3.7; 95% CI: 1.23–7.30], not being appointed for follow-up [AOR = 2.8; 95% CI: 1.12–6.70], being married [AOR = 2.9; 95% CI: 1.35–6.23], and needing to have more children [AOR = 3.2; 95% CI: 1.5–7.0]
Conclusions
The findings of this study conclude that the overall magnitude of the IUCD discontinuation rates in the study area were found to be high when compared with different studies conducted in Ethiopia. Many of the factors that are attributed to the high magnitude of IUCD discontinuation are changeable. Appropriate counseling prior to insertion, including an appointment for follow-up visits, the preference to have more children, and marital status for the choice of service, will improve the continuation rate of IUCD.
Effect of Post-COVID Face-to-Face learning Mode on Endodontic Students’ Satisfaction and Exam Performance Compared to Hybrid Learning Mode during the pandemic; a Single-centre Retrospective Study.
Authors: Elemam Ranya , Elsewify Tarek , Eid Bassem 
Published: April 2023

Abstract
Aim
This study compared the perceptions and exam performance of third-year undergraduates in endodontic courses. The study analyzed hybrid training during COVID-19 and Face-to-Face teaching post-COVID-19 to identify the most effective teaching methods.
Materials and Methods
This study was a single-centre retrospectively analyzed endodontic courses delivered to third-year dental students in 2021 and 2022 at the Gulf Medical University, College of Dentistry. Student perceptions of the hybrid and F2F courses were obtained through a satisfaction questionnaire administered by the University's Quality Assurance Office at the end of each course. Student performance was measured by analyzing exam grades for both teaching modes.
Results
Total of 75 students took the hybrid course while 77 received the F2F course. The number of respondents to the questionnaire was significantly higher for the F2F group (81% vs. 31.5%, p ≤ 0.05). The hybrid course received a satisfaction score of 100% compared to 94.54% for F2F. Student performance in the hybrid group was comparable to the F2F group, with mid- and end-of-year course marks ranging from 64.67% to 71.46% and 63.93% to 65.79%, respectively (p ≤ 0.05). These findings suggest that the hybrid model did not negatively affect student performance.
Conclusion.
In conclusion, the hybrid learning approach was well-received and produced similar endodontic exam marks to F2F teaching. This suggests that combining e-learning with F2F teaching can maintain the quality of education. The absence of differences between the groups dismisses concerns that endodontic education suffered due to the pandemic.
WITHDRAWN: Control Algorithm Validation Using PLC based Hardware Test-Bench for Stable Microgrid Automation
Authors: Mehta Sahil , Basak Prasenjit 
Published: April 2023

Abstract

With the advancements in automation-based technology in engineering applications, programmable logic controller (PLC) has gained much attention. These PLCs possess real-time on-field applicability, reliable operation, long life, robust behavior and quick action in response to various inputs, multiple decision-making variables, harsh environmental conditions, etc. However, unlike the simulation software, tool boxes, and other setups, a robust and reconfigurable PLC can be employed to validate control algorithms for microgrids considering any geographical and environmental conditions. Thus, this paper proposes a PLC-based hardware test bench prototype as an effective solution for control algorithm validation aiming at power management problems and stable microgrid automation. The critical contribution of this paper includes the design and fabrication of the controller test bench prototype, abstracting it as the microgrid central controller (MGCC). The energy management system for the microgrid model with the fuzzy logic-based controller has been developed using MATLAB Simulink software. The performance of the proposed controller test bench is analyzed considering perturbations due to reduced renewable resource contribution. The results validate the proposed controller’s control algorithms, which can ensure the stable operation of the microgrid.
A Simple Framework for Likely Climate Projections Applied to Tropical Width
Authors: Baldassare Daniel , Reichler Thomas 
Published: April 2023

Abstract
Confronted with the difficult task of choosing between a wide range of climate models and emission scenarios, scientists are often left questioning which models and scenarios are most representative of our future climate. Currently, most climate projections use the highest emission scenario and an evenly weighted ensemble of all available climate models despite substantial evidence that the continuously rising emissions in the high emission scenario are unrealistic, and that some models are more reliable than others. As a result, unlikely climate projections have been presented as our likely future. Here, we present a simple and easy to use framework for creating projections of our likely future climate using available model data. The framework involves three measures: selecting the most likely emission scenario, choosing the most reliable models, and debiasing against reanalysis data. To demonstrate our framework and its components, we estimate future changes in tropical width, a hydrologically important climate feature. Our projections show that the likely tropical expansion is roughly half of previous estimates. This framework can be easily applied to other climate features, allowing for better estimates of likely future conditions.
Neuroanatomical Predictors of Alcohol Consumption in Adolescents via in vivo Structural Imaging: A Systematic Review of Longitudinal Studies
Authors: Honarvar Faraz , Arfaie Saman , Edalati Hanie , Ghasroddashti Arashk , Solgi Arad , Mashayekhi Mohammad Sadegh , Mofatteh Mohammad , Ren Lily Yuxi , Kwan Angela Tian Hui , Keramatian Kamyar 
Published: April 2023

Abstract
Aims
This study aimed to systematically review the literature on neuroanatomical predictors of future problematic drinking in adolescents.
Methods
Using PRISMA guidelines, a systematic review was conducted to evaluate neuroanatomical predictors of problematic drinking in adolescents. Embase, MEDLINE, and PsycINFO databases were searched from inception to January 6th, 2023. Studies were included if they were original, had a prospective design, had a sample size of at least 12, had a follow-up period of at least one year, had at least one structural neuroimaging scan before 18 with no prior alcohol use, and had alcohol use as the primary outcome. Studies were excluded if: had animals only; and were not in English. Risk of bias was conducted using the CASP tool.
Results
Out of 1,412 studies identified, 19 studies met the criteria, consisting of eleven gray matter (n = 4,040), five white matter (n = 319), and three assessing both (n = 3,608). Neuroanatomical predictors of future problematic drinking in adolescents were reported to be distributed across various brain regions such as the orbitofrontal cortex and paralimbic regions. However, the findings were largely heterogeneous.
Conclusions
This is the first systematic review to map out the existing literature on neuroanatomical predictors of problematic drinking in adolescents. Future research should focus on the aforementioned regions to determine their role in predicting future problematic drinking with more certainty.
Evidence for Involvement of the Alcohol Consumption WDPCP Gene in Lipid Metabolism, and Liver Cirrhosis
Authors: Pazoki Raha , O’Farrel Felix , Aleyakpo Benjamin , Mustafa Rima , Jiang Xiyun , Pinto Rui , Elliott Paul , Tzoulaki Ioanna , Abbas Dehghan , Loh Samantha , Barclay Jeff , Martins Luis 
Published: April 2023

Abstract
Alcohol consumption continues to cause a significant health burden globally. The advent of genome-wide association studies has unraveled many genetic loci associated with alcohol consumption. However, biological effects of these loci and pathways involved in alcohol consumption and its health consequences such as alcohol liver disease (ALD) remain to be elucidated. We combined human studies with model organisms Drosophila melanogaster and Caenorhabditis elegans to shed light on molecular mechanisms underlying alcohol consumption and the health outcomes caused by alcohol intake. Using genetics and metabolite data within the Airwave study, a longitudinal study to investigate the health of employees of police forces in the UK, we performed several analyses to identify changes in circulating metabolites that are triggered by alcohol consumption and found an enrichment of the alcohol-associated metabolites within the linoleic acid (LNA) and alpha-linolenic acid (ALA) metabolism pathways. We identified evidence of a potential causal relationship between alcohol consumption with several triradylglycerols (TAGs) and diradylglycerols (DAGs), a fatty ester (CAR DC18:1), an sphingomyelin (SM 40:2;O2), and an alkaloid (Piperine). We selected a set of genes annotated to genetic variants that (1) are known to be implicated in alcohol consumption, (2) are linked to liver function, and (3) are associated with the expression (cis-eQTL) of their annotated genes. We used mutations and/or RNA interference (RNAi) to suppress the expression of these genes in C. elegans and Drosophila. Testing the differences in locomotion of C. elegans showed that RNAi knockdown of ACTR1B and MAPT reduced locomotion rate in C. elegans after exposure to ethanol. We showed that RNAi knockdown of several genes (WDPCP, TENM2, GPN1, ARPC1B, SCN8A) in Drosophila changed the sedative effect of ethanol whilst RNAi knockdown of TENM2 reduced ethanol consumption. We also investigated alcohol-induced changes in TAG levels in Drosophila and demonstrated that RNAi knockdown of WDPCP, TENM2 and GPN1 reduce TAG levels. Finally, using publicly available human data, we showed that gene expression of WDPCP is linked to liver fibrosis and liver cirrhosis. Our results underline the impact of alcohol consumption on the metabolism of lipids and pinpoint WDPCP as a gene with a potential impact on lipid accumulation upon exposure to ethanol suggesting a possible pathway to ALD.
Panax notoginseng Saponins Alleviate LPS-induced Fibrosis of HK-2 Cells
by Inhibiting the Activation of NLRP3 Inflammasome and Pyroptosis
Authors: Xie Jing , Ma Xin , Li Xueying , Mao Nan , Ren Sichong , Fan Junming 
Published: April 2023

Background:
Renal fibrosis is related to impaired kidney function and can eventually
lead to end-stage renal disease, for which no effective treatment is available. Panax notoginseng
saponins (PNS), as a commonly used traditional Chinese medicine, is considered a possible alternative
for the treatment of fibrosis.

Objective:
The purpose of the present study was to investigate the effects and possible mechanisms
of PNS on renal fibrosis.

Methods:
HK-2 cells were used to induce renal fibrosis cell model by lipopolysaccharide (LPS),
and the cytotoxicity of PNS on HK-2 cells was investigated. Cell damage, pyroptosis, and fibrosis
were analyzed to investigate the effects of PNS on LPS-induced HK-2 cells. NLRP3 agonist Nigericin
was used further to explore the inhibitory effect of PNS on LPS-induced pyroptosis so as
to clarify the possible mechanism of PNS on renal fibrosis.

Results:
PNS had no cytotoxicity on HK-2 cells, and could reduce the apoptosis and the release of
lactate dehydrogenase (LDH) and inflammatory cytokines of LPS-induced HK-2 cells, showing an
alleviating effect on cell damage. PNS also reduced the expression of pyroptosis proteins NLRP3,
IL-1β, IL-18, and Caspase-1, as well as fibrosis proteins α-SMA, collagen Ⅰ and p-Smad3/Smad3,
which showed an inhibitory effect on LPS-induced pyroptosis and fibrosis. In addition, LPSinduced
cell damage, pyroptosis, and fibrosis were aggravated after Nigericin treatment, while
PNS alleviated the aggravation caused by Nigericin.

Conclusion:
PNS inhibited pyroptosis by inhibiting the activation of NLRP3 inflammasome in
LPS-induced HK-2 cells, which ultimately alleviated renal fibrosis and played a good role in the
treatment of kidney diseases.