bluuebunny/crossref_metadata_2025_split huggingface dataset:
The gut microbiome of obese postpartum women with and without previous gestational diabetes mellitus and the gut microbiota of their babies
Authors: Dualib Patricia Medici , Fernandes Gabriel , Taddei Carla R. , Carvalho Camila R. S. , Sparvoli Luiz Gustavo , Bittencourt Célia , Silva Isis T. , Mattar Rosiane , Ferreira Sandra R. G. , Dib Sergio A. , de Almeida-Pititto Bianca 
Published: December 2022

Abstract
Background
The incidence of gestational diabetes mellitus (GDM) is increasing worldwide, and has been associated with some changes in the gut microbiota. Studies have shown that the maternal gut microbiota pattern with hyperglycemia can be transmitted to the offspring. The study aimed to evaluate the gut microbiota of obese postpartum women with and without previous GDM and their offspring.

Methods
We evaluated a total of 84 puerperal women who had (n = 40) or not GDM (n = 44), and their infants were also included. Stool samples were obtained 2–6 months after delivery. The molecular profile of the fecal microbiota was obtained by sequencing V4 region of 16S rRNA gene (Illumina® MiSeq).

Results
We found that the gut microbiota structures of the puerperal women and their infants were similar. Stratifying according to the type of delivery, the relative abundance of Victivallis genus was higher in women who had natural delivery. Exposure to exclusive breastfeeding was associated with a greater abundance of Bacteroides and Staphylococcus. The differential abundance test showed correlations to clinical and laboratory parameters. This work showed no difference in the microbiota of obese puerperal women with and without GDM and their offspring. However, breastfeeding contributed to the ecological succession of the intestinal microbiota of the offspring.

Conclusion
This work can contribute to understanding the potential effects of GDM and early life events on the gut microbiome of mothers and their offspring and its possible role in metabolism later in life.
Immunoglobulin replacement therapy in patients with immunodeficiencies: impact of infusion method on patient-reported outcomes
Authors: Mallick Rajiv , Solomon Geneviève , Bassett Paul , Zhang Xiang , Patel Palak , Lepeshkina Oleksandra 
Published: December 2022

AbstractBackgroundUnderstanding the impact of different immunoglobulin (Ig) infusion methods (intravenous [IVIg] and subcutaneous [SCIg]) upon treatment experience can potentially facilitate optimization of patient outcomes. Here, the perspective of patients with primary and secondary immunodeficiency diseases (PID and SID, respectively) receiving IVIg and SCIg was evaluated, in terms of treatment satisfaction, accounting for treatment history, using Association des Patients Immunodéficients du Québec (APIQ) survey data.MethodsThe online APIQ survey (shared October 2020–March 2021) of patients with immunodeficiencies in Canada contained 101 questions on: Ig use, history, and detailed infusion characteristics; as well as structured patient-reported outcomes such as treatment satisfaction (via TSQM-9), symptom state (via PASS), general health perception (via GHP), and physical and mental function (via PROMIS). Adult respondents (≥ 18 years old) currently using Ig were compared by their current Ig infusion method (IVIg or SCIg cohort) overall, and in a sub-analysis, the IVIg cohort was compared with the SCIg cohort after stratification by respondents who started SCIg when naïve to Ig (‘SCIg naïve’) or with previous IVIg experience (‘SCIg switch’).ResultsIn total, 54 respondents currently used IVIg and 242 used SCIg. The average duration per infusion of a weekly SCIg infusion was significantly shorter compared with the average duration of a 3–4 weekly IVIg infusion (p < 0.001). The SCIg cohort was associated with significantly higher scores for the TSQM-9effectivenessdomain compared with the IVIg cohort. The scores for TSQM-9convenienceandglobal satisfactiondomains were similar in the two cohorts. The SCIg cohort was also associated with a significantly higher proportion of respondents who were in an acceptable symptom state and a lower proportion who reported very poor or poor perception of health compared with the IVIg cohort. Further, the SCIg naïve subgroup was associated with significantly higher TSQM-9effectivenessandconveniencedomain scores compared with the IVIg cohort, while there was no significant difference between the SCIg switch subgroup and the IVIg cohort in terms ofconvenience.ConclusionsA better understanding of how different IgRT administration methods impact treatment experience and satisfaction may assist with informed treatment decision making and ultimately further improvements in patient outcomes.
Novel PGM3 mutation in two siblings with combined immunodeficiency and childhood bullous pemphigoid: a case report and review of the literature
Authors: Fallahi Mazdak , Jamee Mahnaz , Enayat Javad , Abdollahimajd Fahimeh , Mesdaghi Mehrnaz , Khoddami Maliheh , Segarra-Roca Anna , Frohne Alexandra , Dmytrus Jasmin , Keramatipour Mohammad , Mansouri Mahboubeh , Eslamian Golnaz , Fallah Shahrzad , Boztug Kaan , Chavoshzadeh Zahra 
Published: December 2022

Abstract
Background
Bullous pemphigoid is the most common autoimmune subepidermal blistering disorder with a low incidence in childhood. Combined immunodeficiencies (CIDs) are a group of monogenic inborn errors of immunity (IEIs) characterized by T- and B-cell dysfunction leading to recurrent infections, lymphoproliferation, predisposition to malignancy, and autoimmunity. Here, we report two Afghan siblings with a diagnosis of CID and extremely rare manifestation of diffuse bullous pemphigoid skin lesions.

Case presentation
The older sibling (patient 1) was a 32-month-old male with facial dysmorphism, protracted diarrhea, failure to thrive, recurrent oral candidiasis, recurrent otitis media with tympanic membrane perforation, who had been previously diagnosed with CID. While he was under treatment with intravenous immunoglobulin (IVIg), he developed extensive blistering lesions, which were diagnosed as childhood bullous pemphigoid. Methylprednisolone and azathioprine were added to the regimen, which resulted in a remarkable improvement of the skin lesions and also the feeding condition. However,2 weeks later, he was re-admitted to the intensive care unit (ICU) and eventually died due to fulminant sepsis. Later, his 12-month-old sister (patient 2) with similar facial dysmorphism and a history of developmental delay, food allergy, recurrent oral candidiasis, and respiratory tract infections also developed blistering skin lesions. She was under treatment for occasional eczematous lesions, and had been receiving IVIg for 3 months due to low levels of immunoglobulins. Further immunologic workup showed an underlying CID and thus treatment with IVIg continued, gradually improving her clinical condition. The genetic study of both siblings revealed a novel homozygous mutation in exon 7 of the PGM3 gene, c.845 T > C (p.Val282Ala).

Conclusions
Dermatologic disorders may be the presenting sign in patients with CID and mutated PGM3. This case report further extends the spectrum of skin manifestations that could be observed in PGM3 deficiency and emphasizes the importance of considering CIDs during the assessment of skin disorders, particularly if they are extensive, recurrent, refractory to treatment, and/or associated with other signs of IEIs.
High isolation MIMO antenna for 5G C-band application by using combination of dielectric resonator, electromagnetic bandgap, and defected ground structure
Authors: Sandi Efri , Diamah Aodah , Al Mawaddah Mumtaz 
Published: December 2022

AbstractHigh isolation between massive MIMO antenna elements is one of the important parameters that improves antenna performance, especially for 5G communication applications. In this study, we propose a design to improve isolation between elements to enhance the antenna performance. The proposed solution to improve the performance of massive MIMO antennas is to use a combination of dielectric resonator, electromagnetic bandgap (EBG) and defected ground structure (DGS) techniques at the frequency band 3.5 GHz as the 5G frequency band under 6 GHz. The material used is FR-4 which has a dielectric constant ($${\varepsilon }_{r}$$

ε
r

) of 4.3. Simulation results and measurements between antenna elements show an improvement in mutual coupling, widening the bandwidth and increasing the gain of the antenna. The proposed design using the dielectric resonator antenna (DRA) by MIMO 8 × 8 16 port—64 elements and the addition of EBG and DGS structures on the ground plane—has shown to suppress mutual coupling parameter lower than without using DRA-EBG-DGS design by 15 dB, increase bandwidth to 246 MHz, increase gain to 24.7 dB and improve the overall envelope correlation coefficient (ECC) parameter.
Investigating the most essential skills of planners in Ethiopia’s Amhara National Regional State’s Regiopolitan cities
Authors: Workineh Nigatu Amsalu 
Published: December 2022

AbstractThis study intends to answer the specific skills and skills Ethiopian planners, particularly Amhara National Regional State's Regiopolitan cities planners, require the most in their urban planning profession. In today's urban world, understanding city dynamics, forecasting and predicting vibrant cities, making urban planning decisions and putting them into practice, collaborating with stakeholders from various governments and educational backgrounds, persuading people to accept urban plans, and building good personal and skilled relationships have all become highly complex and challenging. As a result, to succeed in their jobs in Ethiopia, planners must possess particular skills. This study looks into the essential skills that planners require in today's dynamic and complex urban environment. The investigation was conducted in Ethiopia as part of a more extensive regional study of Regiopolitan cities. A structured questionnaire survey of 104 experienced planners was undertaken, and semi-structured in-depth interviews with 9 key informants to investigate the essential planners' skills in the Ethiopian planning system to find the essential skills needed in practice. The findings demonstrate that an urban planning method in Regiopolitan cites of Amhara National Regional State, Ethiopia urban planning system is such practical planners who wish to carve out a position for them must equip themselves with analytical and synthesis skills. Finally, the study included recommendations for improving planners’ future skills and more research into the matter.
Research progress on the mechanism of interleukin-1β on epiphyseal plate chondrocytes
Authors: Tong Ziyuan , Yang Xu , Li Jianjun 
Published: December 2022

AbstractEpiphyseal plate injury, a common problem in pediatric orthopedics, may result in poor bone repair or growth defects. Epiphyseal plate, also known as growth plate is a layer of hyaline cartilage tissue between the epiphysis and metaphyseal and has the ability to grow longitudinally. Under normal physiological conditions, the epiphyseal plate has a certain axial resistance to stress, but it is fragile in growth phase and can be damaged by excessive stress, leading to detachment or avulsion of the epiphysis, resulting in life-long devastating consequences for patients. There is an obvious inflammatory response in the phase of growth plate injury, the limited physiological inflammatory response locally favors tissue repair and the organism, but uncontrolled chronic inflammation always leads to tissue destruction and disease progression. Interleukin-1β (IL-1β), as representative inflammatory factors, not only affect the inflammatory phase response to bone and soft tissue injury, but have a potentially important role in the later repair phase, though the exact mechanism is not fully understood. At present, epiphyseal plate injuries are mainly treated by corrective and reconstructive surgery, which is highly invasive with limited effectiveness, thus new therapeutic approaches are urgently needed, so a deeper understanding and exploration of the pathological mechanisms of epiphyseal plate injuries at the cellular molecular level is an entry point. In this review, we fully introduced the key role of IL-1 in the progression of epiphyseal plate injury and repair, deeply explored the mechanism of IL-1 on the molecular transcript level and endocrine metabolism of chondrocytes from multiple aspects, and summarized other possible mechanisms to provide theoretical basis for the clinical treatment and in-depth study of epiphyseal plate injury in children.
Enhanced delivery of a low dose of aducanumab via FUS in 5×FAD mice, an AD model
Authors: Kong Chanho , Yang Eun-Jeong , Shin Jaewoo , Park Junwon , Kim Si-Hyun , Park Seong-Wook , Chang Won Seok , Lee Chang-Han , Kim Hyunju , Kim Hye-Sun , Chang Jin Woo 
Published: December 2022

AbstractBackgroundAducanumab (Adu), which is a human IgG1 monoclonal antibody that targets oligomer and fibril forms of beta-amyloid, has been reported to reduce amyloid pathology and improve impaired cognition after administration of a high dose (10 mg/kg) of the drug in Alzheimer’s disease (AD) clinical trials. The purpose of this study was to investigate the effects of a lower dose of Adu (3 mg/kg) with enhanced delivery via focused ultrasound (FUS) in an AD mouse model.MethodsThe FUS with microbubbles opened the blood–brain barrier (BBB) of the hippocampus for the delivery of Adu. The combined therapy of FUS and Adu was performed three times in total and each treatment was performed biweekly. Y-maze test, Brdu labeling, and immunohistochemical experimental methods were employed in this study. In addition, RNA sequencing and ingenuity pathway analysis were employed to investigate gene expression profiles in the hippocampi of experimental animals.ResultsThe FUS-mediated BBB opening markedly increased the delivery of Adu into the brain by approximately 8.1 times in the brains. The combined treatment induced significantly less cognitive decline and decreased the level of amyloid plaques in the hippocampi of the 5×FAD mice compared with Adu or FUS alone. Combined treatment with FUS and Adu activated phagocytic microglia and increased the number of astrocytes associated with amyloid plaques in the hippocampi of 5×FAD mice. Furthermore, RNA sequencing identified that 4 enriched canonical pathways including phagosome formation, neuroinflammation signaling, CREB signaling and reelin signaling were altered in the hippocami of 5×FAD mice receiving the combined treatment.ConclusionIn conclusion, the enhanced delivery of a low dose of Adu (3 mg/kg) via FUS decreases amyloid deposits and attenuates cognitive function deficits. FUS-mediated BBB opening increases adult hippocampal neurogenesis as well as drug delivery. We present an AD treatment strategy through the synergistic effect of the combined therapy of FUS and Adu.
Global patterns of diversity and metabolism of microbial communities in deep-sea hydrothermal vent deposits
Authors: Zhou Zhichao , St. John Emily , Anantharaman Karthik , Reysenbach Anna-Louise 
Published: December 2022

AbstractBackgroundWhen deep-sea hydrothermal fluids mix with cold oxygenated fluids, minerals precipitate out of solution and form hydrothermal deposits. These actively venting deep-sea hydrothermal deposits support a rich diversity of thermophilic microorganisms which are involved in a range of carbon, sulfur, nitrogen, and hydrogen metabolisms. Global patterns of thermophilic microbial diversity in deep-sea hydrothermal ecosystems have illustrated the strong connectivity between geological processes and microbial colonization, but little is known about the genomic diversity and physiological potential of these novel taxa. Here we explore this genomic diversity in 42 metagenomes from four deep-sea hydrothermal vent fields and a deep-sea volcano collected from 2004 to 2018 and document their potential implications in biogeochemical cycles.ResultsOur dataset represents 3635 metagenome-assembled genomes encompassing 511 novel and recently identified genera from deep-sea hydrothermal settings. Some of the novel bacterial (107) and archaeal genera (30) that were recently reported from the deep-sea Brothers volcano were also detected at the deep-sea hydrothermal vent fields, while 99 bacterial and 54 archaeal genera were endemic to the deep-sea Brothers volcano deposits. We report some of the first examples of medium- (≥ 50% complete, ≤ 10% contaminated) to high-quality (> 90% complete, < 5% contaminated) MAGs from phyla and families never previously identified, or poorly sampled, from deep-sea hydrothermal environments. We greatly expand the novel diversity of Thermoproteia, Patescibacteria (Candidate Phyla Radiation, CPR), and Chloroflexota found at deep-sea hydrothermal vents and identify a small sampling of two potentially novel phyla, designated JALSQH01 and JALWCF01. Metabolic pathway analysis of metagenomes provides insights into the prevalent carbon, nitrogen, sulfur, and hydrogen metabolic processes across all sites and illustrates sulfur and nitrogen metabolic “handoffs” in community interactions. We confirm that Campylobacteria and Gammaproteobacteria occupy similar ecological guilds but their prevalence in a particular site is driven by shifts in the geochemical environment.ConclusionOur study of globally distributed hydrothermal vent deposits provides a significant expansion of microbial genomic diversity associated with hydrothermal vent deposits and highlights the metabolic adaptation of taxonomic guilds. Collectively, our results illustrate the importance of comparative biodiversity studies in establishing patterns of shared phylogenetic diversity and physiological ecology, while providing many targets for enrichment and cultivation of novel and endemic taxa.
Virus diversity and interactions with hosts in deep-sea hydrothermal vents
Authors: Cheng Ruolin , Li Xiaofeng , Jiang Lijing , Gong Linfeng , Geslin Claire , Shao Zongze 
Published: December 2022

AbstractBackgroundThe deep sea harbors many viruses, yet their diversity and interactions with hosts in hydrothermal ecosystems are largely unknown. Here, we analyzed the viral composition, distribution, host preference, and metabolic potential in different habitats of global hydrothermal vents, including vent plumes, background seawater, diffuse fluids, and sediments.ResultsFrom 34 samples collected at eight vent sites, a total of 4662 viral populations (vOTUs) were recovered from the metagenome assemblies, encompassing diverse phylogenetic groups and defining many novel lineages. Apart from the abundant unclassified viruses, tailed phages are most predominant across the global hydrothermal vents, while single-stranded DNA viruses, including Microviridae and small eukaryotic viruses, also constitute a significant part of the viromes. As revealed by protein-sharing network analysis, hydrothermal vent viruses formed many novel genus-level viral clusters and are highly endemic to specific vent sites and habitat types. Only 11% of the vOTUs can be linked to hosts, which are the key microbial taxa of hydrothermal habitats, such asGammaproteobacteriaandCampylobacterota. Intriguingly, vent viromes share some common metabolic features in that they encode auxiliary genes that are extensively involved in the metabolism of carbohydrates, amino acids, cofactors, and vitamins. Specifically, in plume viruses, various auxiliary genes related to methane, nitrogen, and sulfur metabolism were observed, indicating their contribution to host energy conservation. Moreover, the prevalence of sulfur-relay pathway genes indicated the significant role of vent viruses in stabilizing the tRNA structure, which promotes host adaptation to steep environmental gradients.ConclusionsThe deep-sea hydrothermal systems hold untapped viral diversity with novelty. They may affect both vent prokaryotic and eukaryotic communities and modulate host metabolism related to vent adaptability. More explorations are needed to depict global vent virus diversity and its roles in this unique ecosystem.
Effects of acidic phosphorus-rich biochar from halophyte species on P availability and fractions in alkaline soils
Authors: Wang Xuyang , Sun Tao , Ma Haigang , Tang Guangmu , Chen Mo , Abulaizi Maidinuer , Yu Guangling , Jia Hongtao 
Published: December 2022

AbstractConditioning alkaline soil with acidic phosphorus-rich biochar might contribute to improving soil phosphorous (P) availability and waste utilization efficiency. In this study, acidic phosphorus-rich biochar was prepared using halophyte biochar (HBC) modified by H3PO4 (P-HBC) and H4P2O7 (PA-HBC). The P-containing groups and P fractions in the HBCs were characterized, and the effects of biochar on the P fractions in alkaline soil were examined with incubation experiments. The results showed that the pH of P-HBC (3.31) and PA-HBC (2.17) was significantly reduced compared with HBC, and the total P contents rose to 4.66 g·kg− 1 and 5.24 g·kg− 1, respectively. The spectral characterization confirmed the loading of P-containing and acidic functional groups in the HBCs after modification. The application of P-HBC and PA-HBC in alkaline soils decreased the soil pH and facilitated the transformation of stable P into active fractions. Overall, acidic phosphorus-rich biochar can be employed to increase P activity in alkaline soil.
Graphical Abstract
Gold nanoparticles loaded on TiO2 nanoparticles doped with N2 as an efficient electrocatalyst for glucose oxidation: preparation, characterization, and electrocatalytic properties
Authors: Jamil Yasmin M. S. , Awad Mohammed Ahmed Hussein , Al-Maydama Hussein M. A. , Alhakimi Ahmed N. , Shakdofa Mohamad M. E. , Mohammed Samir Osman 
Published: December 2022

AbstractA powder of titanium oxide nanoparticles (TiO2 NPs) was synthesized in this study by anodizing in 0.7 M HClO4 and then annealing in N2 at 450 °C for 3 h to produce TiO2 NPs-N2 powder as a catalyst. These TiO2 NPs-N2 nanoparticles were then encrusted with Au nanoparticles utilizing the photodeposition procedure with tetrachloroauric acid (HAuCl4) and isopropanol as sacrificial donors. With a surface area of 121 m2g−1, the Au NPs/TiO2 NPs-N2 powder catalyst has a high surface area, according to the Barrett–Joyner–Halenda technique. According to X-ray diffraction (XRD) analysis, TiO2 NPs-N2 contained uniformly integrated Au nanoparticles with an average crystallite size of about 26.8 nm. The XRD patterns showed that the prepared Au NPs/TiO2 NPs-N2 were crystallites and nano-sized. The transmission electron microscopy image revealed the spherical shape of the nanoparticles and their tendency for agglomeration. Utilizing the cyclic voltammetry, the electrochemical properties of the catalyst TiO2 NPs powders in a basic glucose solution were investigated. The electrocatalytic activity and stability of the loaded Au NPs/TiO2 NPs-N2 powder on the working electrode for the electrocatalytic oxidation of glucose were astonishingly high. The Au NPs/TiO2 NPs-N2 catalyst demonstrated electrocatalytic characteristics that were superior to a commercially available polycrystalline gold electrode in the application involving glucose alkaline fuel cells.