bluuebunny/crossref_metadata_2025_split huggingface dataset:
Voices of children adopted at an older age about challenges and facilitators in their social history
Authors: Bejenaru Anca , Iovu Mihai , Raiu Sergiu 
Published: June 2023

Abstract
Since 2016, the adoption of older children has become a priority for policymakers and practitioners in Romania, and several measures have been taken to stimulate their adoption. Since this is a relatively new practice in Romania, we were not able to identify any studies that gave a voice to Romanian children to understand how they experience adoption and the challenges and facilitator factors that entail. This study aimed to fill this gap by interviewing 12 children and adolescents aged between 7 and 13. The children offered important insights into the risk factors and adversities they endured before adoption, the challenges they faced during this process, and the tasks they had to undertake after. They also highlighted the factors that facilitated their resilience throughout the adoption process. We found that older children, despite their past adversities, have a good capacity for resilience, which is reflected in their ability to create new bonds based on trust and security with their adoptive parents, in their self-satisfaction and awareness of the progress they have made after adoption, and in their confidence and future aspirations. Finally, based on the interviews, we formulated recommendations for practice and social policies.
An Optimized Machine Learning Model for the Early Prognosis of Chronic Respiratory Diseases with specific focus On Asthma
Authors: Pooja M R , Giri Jayant , Chadge Rajkumar , Sunheriya Neeraj , Mahatme Chetan , mallik Saurav , Alsubaie Najah , Alqahtani Mohammed S. , Abbas Mohamed , Soufiene Ben Othman 
Published: June 2023

Abstract
Clinicians and those into the field of clinical research often evaluate the benefits against costs and inconvenience of preventive interventions for asthma by performing risk identification and assessment through the application of Artificial Intelligence (AI) based predictive models. As such, a Weighted Feature Averaging Technique (WFAT), a novel feature extraction strategy that uniquely identifies the risk factors contributing to asthma, by taking into consideration the various aspects of weighing the importance of features with respect to their predictive capabilities. We introduce a weighted feature averaging technique to extract the most relevant and predominant features that score well on their relevance with respect to asthma outcome, the target for the problem under study. To strengthen the classification performance, ensemble machine learning techniques centric to AI techniques that are widely known to do so are explored and a new ensemble machine learning model, which is seen to outperform the weak classifiers by deploying optimization techniques that yield comparatively better results has been proposed. The ensemble model proposed yielded a classification accuracy as high as 85% and 91% with two diverse datasets chosen for modeling early prognosis of asthma disease by deploying only 25% of the features in the reduced feature set.
Biosynthesis, characterization of silver nano particles from Agaricus bisporus (Mushroom) and their antimicrobial activity against foodborne bacterial pathogens
Authors: Amr Maryam , Abd-Elhalim Basma T. , Ismail Radwa , Aboubakr Asmaa , Wael Rahma , Yasser Mariam , Hemdan Bahaa , El-Sayed Salwa M. , Abu-Hussien Samah H. , Bakry Ashraf , Ebeed Naglaa , Elhariry Hesham , Galal Ahmed 
Published: June 2023

Abstract
Biosynthesized silver nanoparticles (AgNPs) attracted broad interest in the food sector due to their excellent antimicrobial activities as a new alternative to harmful chemical disinfectants. This work aims to study characterization parameters, antimicrobial and toxicological characteristics of the biosynthesized silver nanoparticles from Agaricus bisporus (Mushroom) aqueous extract. Mushroom extract was treated with silver nitrate and incubated for 24h until its color was turned brown. AgNPs characterization studies were estimated for their stability, their characteristics using UV and visible spectroscopy, X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), high-resolution transmission electron microscopy (HR-TEM), Dynamic light scattering (DLS) and energy dispersive X-ray spectroscopy (XRD). All synthesized particles had the maximum absorption at 456nm at 1.5 Surface plasmon resonance (SPR), indicating the biosynthesis of nano silver particles. In addition. AgNPs from A. bisporus (Mushroom) exhibited broad antibacterial action against eight foodborne bacterial strains. Among all the tested bacterial strains, P. aeruginosa ATCC 27853 was the superlative sensitive strain with inhibition zone of 48mm, while IZD for K. pneumonia ATCC 700603 and B. cereus ATCC 11778 were the most resistant strains with IZD of 20mm. According to the MIC/MBC ratio, the silver cations released by AgNPs had strong bactericidal effects against both G+ ve and G− ve bacteria. Cytotoxicity test against normal human skin fibroblast indicated that silver nano silver particles from the mushroom extract were safe, exhibiting cell viability reached 98.2%. Therefore, AgNPs could be a good alternative source to inhibit the biofilm formation in the food industry sector.
Identifying the Glaze Structure of Historical Clay Bricks: A Case Study of Persepolis Clay Bricks
Authors: Samanian Sasan , Bahmani Sareh 
Published: June 2023

Abstract
Glazes include a wide range of organic and inorganic compounds. During the Achaemenid period, one of the decoration methods of buildings and palaces was the use of colored glazed clay bricks. This experimental-laboratory research was carried out regarding valid scientific documents.
Purpose: to identify the constituent elements of glazes in Achaemenid clay bricks.
Research Question: What are the constituent elements and the main coloring factors in gray and azure blue glazes of Achaemenid clay bricks in Persepolis?
This research aims to identify the structure of the glaze of Persepolis clay bricks. Among the different devices that are used to identify minerals, X-Ray Diffraction and Scanning Electron Microscope laboratory methods were used. The XRD device was used to detect the type of material and determine the type of constituent phases and the SEM device was used to determine the amount of constituent elements of the material. The glazes of Persepolis bricks are white, pale green, dark green, dark brown, gray, fawn yellow, and azure blue. In this article, the structure of gray and azure blue glazes was identified and studied. The study samples were physically sampled as a tiny flake-shaped layer of glaze from the building body on the eastern wall of the exclusive women's palace during the time of Xerxes. In total, three samples of gray glaze and three samples of azure blue glaze were selected. According to the results, the existence of silica and porosity of the body and also the existence of iron and magnesium in the composition of the glazes were confirmed, which were probably present in the original composition (base glaze), showing that besides the color effects, they were also present in them as impurities. Based on the structure of gray and azure blue glazes, both glazes are alkaline. The scientific and academic application of this research is for the protection and restoration of brick glazes and also for the use of the Ministry of Cultural Heritage, Handicrafts, and Tourism, as well as students in restoration of cultural and historical artefacts in Art universities.
Rationale for issuing neuroimaging requests for patients with primary headaches: a cross-sectional survey of hospitals and clinicians in China
Authors: Zhong Xuemin , Zhao longchao , Peng Linli , Li Li , Li Changqing 
Published: June 2023

Abstract
Background
Neuroimaging is overused globally in patients with primary headaches. Evidence of this is lacking in China. We aimed to investigate the prevalence of neuroimaging in patients with primary headaches and the clinician-based rationale for requesting neuroimaging in China.
Methods
This was a cross-sectional survey of hospitals and clinicians in China. We used a convenience sampling method to recruit patients with primary headaches admitted to six hospitals in three provincial capitals in 2022. We identified whether neuroimaging had been requested and the types of neuroimaging conducted. We interviewed clinicians using a combination of personal in-depth and topic selection group interviews to explore why doctors request neuroimaging.
Results
Among 2,263 patients recruited for this study, 1,942 (89.75%) underwent neuroimaging. Patients underwent head computed tomography (CT), 628 (27.75%); magnetic resonance imaging (MRI), 1,157 (51.13%); or both CT and MRI, 246 (10.87%). Fifteen of the 16 interviewed clinicians would not issue a neuroimaging request for patients with primary headaches. Further, we found that doctors issued a neuroimaging request for patients with primary headaches mostly to exclude the risk of misdiagnosis, reduce uncertainty, avoid medical disputes, meet patients’ medical needs, and complete the hospital assessment indicators.
Conclusions
For primary headaches, the probability of clinicians requesting neuroimaging is higher in China than in other countries. There is considerable room for improvement in finding appropriate strategies for doctors and patients to reduce the use of low-value care.
Symptom Reduction in Advanced Cancer from Multi-session Mindful Breathing: A Randomised Controlled Trial
Authors: Tung Yu-Zhen , Ng Diana-Leh-Ching , Lai Natalie-Zi , Ang Chui-Munn , Lim Poh-Khuen Lim , Zainuddin Sheriza Izwa , Lam Chee-Loong , Loh Ee-Chin , Chai Chee-Shee , Tan Seng-Beng 
Published: June 2023

Abstract

Background: Mindfulness-based interventions have shown promising effects among patients with advanced cancer and other health conditions. While evidence of symptom reduction in palliative care from a single session of mindful breathing is available, data on symptom reduction from multi-session mindful breathing remains unavailable. The objective of this study was to determine the efficacy of multi-session mindful breathing in symptom reduction among patients with advanced cancer.
Methods: Adult patients with advanced cancer who scored ≥4 in at least two or more symptoms based on the Edmonton Symptom Assessment Scale (ESAS) were recruited from January to March 2020. Participants were randomly assigned to receive either four daily sessions of 30-minute mindful breathing and standard care (intervention) or standard care alone (control).
Results: There were statistically significant reductions in the total ESAS scores following all four sessions in the intervention group (n1 = 40: z1 = -5.09, p < 0.001; z2 = -3.77, p < 0.001; z3 = -4.38, p < 0.001; z4 = -3.27, p < 0.05). For the control group, statistically significant reductions in the total ESAS scores were seen only after sessions 1 and 3 (n2 = 40: z1 = -4.04, p < 0.001; z3 = -4.53. p < 0.001).
Conclusions: Our result provides evidence that four daily sessions of 30-minute mindful breathing may be effective in reducing multiple symptoms rapidly in advanced cancer patients.
Trial registration: NCT 05910541, date of registration 9th June 2023. (Retrospectively registered)
Effects of rice-fish symbiosis on the muscle nutrition, intestinal microbes, liver metabolism and of yellow catfish (Pelteobagrus fulvidraco)
Authors: Zhou Ya , He Xianlin , Xue Xiaoshu , Tang Guo , Mei Jie 
Published: June 2023

Abstract

To investigate the effects of different rice growth periods and different farming modes on the muscle nutrition, intestinal microbes and liver metabolome profiling of yellow catfish in rice-fish symbiosis. 16S rDNA and non-targeted metabolomic methods were used to analyze the differences of muscle amino acids and fatty acids, intestinal microbes’ diversity and liver metabolome profiling of yellow catfish in the rice pre-anthesis paddy group (aRY_tac), the rice post-anthesis paddy group (bRY_tac), the post-harvest paddy group (cRY_tac) and the post-harvest pond group (dRY_tac). The result showed that the muscle total amino acids (TAA), non-essential amino acids (TNEAA), and umami amino acids (TDAA) in bRY_tac group were significantly (P < 0.05) higher than those in other groups, the muscle saturated fatty acids in dRY_tac group were significantly (P < 0.05) higher than those of other groups, and the content of monounsaturated fatty acids in cRY_tac group was significantly higher than that of the other groups (P < 0.05). The dominant bacteria in aRY_tac group wasPseudomonas, in bRY_tac group wereClostridium,OxyphotobacteriaandBrevundimonas, in cRY_tac group wereCetobacteriumandCandidatus_Arthromitus, and in dRY_tac group wasPlesiomonas. Yellow catfish cultured in rice-fish symbiosis mode possessed higher abundance and diversity of intestinal microbes than that in pond mode. Moreover, under rice-fish symbiosis mode, compared with the rice pre-anthesis and post-harvest periods, yellow catfish in rice post-anthesis’s period showed higher abundance and diversity of intestinal microbes and the liver metabolic phenotype, especially in Arginine biosynthesis, Monobactam biosynthesis, Cysteine and methionine metabolism and amino acids biosynthesis, etc. Our study suggested that different rice growth periods in the paddy field had different effects on the muscle nutritional components, microbial diversity and liver metabolism of yellow catfish, the fish nutritional quality in rice post-anthesis periods would be better. This study provides a theoretical reference for the cultivation of yellow catfish under rice-fish symbiosis system.
Crosstalk of cell death pathways implicates heterogeneity among molecular subtypes in colorectal cancer
Authors: Weng Siyuan , Cui Haiyang , Xu Hui , Zhang Yuyuan , Wang Libo , Xing Zhe , Liu Zaoqu , Han Xinwei 
Published: June 2023

Abstract
Background The intricate crosstalk of various cell death forms was recently implicated in cancers, laying a foundation for exploring the association between cell death and cancers. Recent evidence has demonstrated that biological networks outperformsnapshot gene expression profiles at discovering promising biomarkers or heterogenous molecular subtypes across different cancer types. In order to investigate the behavioral patterns of cell death-related interaction perturbation in colorectal cancer (CRC), this study constructed the interaction-perturbation network consisting of 11 cell death pathways and further revealed four cell death network (CDN) derived subtypes (CDN1-4).
Methods We constructed a perturbation matrix of 11 cell death pathways by using colorectal cancer data from TCGA and GTEx, from which four CDNs were decoded and validated by three GEO datasets.
Results Four subtypes were well characterized and displayed distinct clinical and molecular features: (1) CDN1: elevated proliferative activity, frequent KRAS mutations, immune desert, and high tumor purity; (2) CDN2: stronger immune activation, best prognosis, high BRAF mutation frequency, high mutational burden, moderate proliferative activity, neoantigen burden, microsatellite instability, and might be sensitive to immunotherapy sensitivity; (3) CDN3: metabolically hyperactive, immune desert, and moderate prognosis; (4) CDN4: stroma-rich, worst prognosis, immune-suppressed advanced stage, strong tumor invasion, stem cell-like, high levels of EMT and TGF-β signaling, and AOC3 considered as a potentially predictive molecule for CDN4.
Conclusions In general, based on the construction of the cell death crosstalk network, which is more stable and effective than gene features, our study established four stable CRC molecular subtypes that could predict prognosis and guided treatment.
Pumilio RNA binding family member 1 deficiency activates anti-tumor immunity in hepatocellular carcinoma via restraining M2 macrophage polarization
Authors: Yu Yang , Nie Gang , ren yi-wei , Ouyang Liu , Ni Chen-ming 
Published: June 2023

Abstract

Pumilio RNA binding family member 1 (PUM1), which has been implicated in both the progression of colorectal cancer and the regulation of Inflammation, has not yet been associated with hepatocellular carcinoma PUM1 is essential for the transition of tumor-associated macrophages (TAMs) into the M2 polarization state. It does this by inhibiting anti-tumor immunity in hepatocellular carcinoma through a process mediated by TAMs that target CD8 + T cells, as demonstrated in this study using PUM1-knockout mice. By activating the cAMP signaling pathway, we have shown that PUM1 promotes the transformation of TAMs into pro-tumorigenic M2-like phenotypes. In order to emphasize the potential of PUM1 as an objective for immunotherapy centered on TAMs in the treatment of gastric carcinoma, the present investigation revealed the molecular mechanism underlying the pro-tumor role of PUM1 in this cancer.
GUCA2A Dysregulation as a Promising Biomarker for Accurate Diagnosis and Prognosis of Colorectal Cancer
Authors: Jalali Pooya , Aliyari Shahram , Taher Sahar , Kavousi Kaveh , Salehi Zahra 
Published: June 2023

Abstract
Background:Colorectal cancer (CRC) is a leading cause of global mortality and presents a significant barrier to improving life expectancy. The primary objective of this study was to discern a unique differentially expressed gene (DEG) that exhibits a strong association with colorectal cancer. By achieving this goal, the research aims to contribute valuable insights to the field of translational medicine.
Methods:We performed an analysis on four colorectal cancer microarray datasets obtained from the GEO database in order to identify differentially expressed genes (DEGs). In addition, we explored the TCGA colon adenoma carcinoma (COAD) dataset using GEPIA2, which provided high-throughput RNA-Seq data to identify DEGs associated with COAD. To further investigate, we conducted a comprehensive analysis using a pan-cancer model encompassing 33 different cancer types to identify common DEGs between the GEO datasets and the GEPIA2 COAD-TCGA data. We also performed gene set enrichment analysis using Enrichr to gain insights into the functional relevance of these DEGs. To uncover potential regulatory relationships, we constructed a co-expression network utilizing data from the STRING and LinkedOmics databases. Furthermore, we established a competing endogenous RNA (ceRNA) network by integrating information from the miRTarBase and circBank databases. Additionally, correlation between tumor-immune signatures in distinct tumor microenvironments was investigated using the TISIDB database. Finally, we investigated potential interactions between the identified gene and various drugs, providing valuable insights into therapeutic possibilities.
Results:GUCA2A emerged as a significant DEG specific to colorectal cancer (|log2FC| > 1 and adjusted q-value < 0.05). Importantly, GUCA2A exhibited excellent diagnostic performance for COAD, with 98% sensitivity, 95% specificity, and a 99.6% area under the curve (AUC). Moreover, low expression of GUCA2A significantly impacted overall patient survival. Enrichment analysis highlighted the receptor guanylyl cyclase signaling pathway and guanylate cyclase activator activity as the most significant gene ontology terms. A ceRNA network consisting of 8 miRNAs targeting GUCA2A and 183 circRNAs acting as miRNA sponges was constructed. Significant correlations were observed between tumor-immune signatures and GUCA2A expression. Additionally, lactose anhydrous, Atropin, and Volanesorsen sodium were identified as drugs potentially interacting with GUCA2A.
Conclusions:This study identifies GUCA2A as a promising prognostic and diagnostic biomarker for colorectal cancer. Further investigations are warranted to explore the potential of GUCA2A as a therapeutic biomarker.
Weekend Hospital Admissions for Myxedema Coma Linked to Higher Mortality Rates: an insight from National Inpatient Sample from 2016 to 2020
Authors: Hashmi Mariam , Bodla Zubair Hassan , Niaz Fatima , Farooq Umer , Bray Christopher L. , Okonoboh Peters 
Published: June 2023

Abstract
Purpose
Our research focuses on utilizing the National Inpatient Sample (NIS) database for investigating the effects of admission day on myxedema coma outcomes.
Methods
This retrospective cohort study examined in-patient mortality and secondary outcomes of patients with myxedema coma from 2016 to 2020 using the NIS database. Patients were selected using International Classification of Diseases, Tenth Revision, Clinical Modification (ICD-10-CM) codes and divided into weekday and weekend admission groups. Statistical analysis was carried out using Stata version 14. Potential confounding variables were accounted for using multivariable logistic and linear regression analysis.
Results
Out of the 174,776,205 discharges, 5,095 patients satisfied the study's inclusion criteria. The average age of admitted patients was slightly higher on weekdays (67.14 year) than weekends (66.52), with females constituting 69.41% and 67.42% of admissions, respectively. Patients admitted on weekends had higher odds of inpatient mortality compared to those admitted on weekdays [adjusted odds ratio (aOR): 1.91, p = 0.009]. The overall mortality rate for myxedema coma rose from 6.76% in 2016 to 13.36% in 2020 (p-value of 0.014). There was no significant difference in the length of stay, total hospitalization charges, hospital cost, blood transfusion, acute kidney injury requiring dialysis, acute respiratory failure requiring intubation and parenteral nutrition between weekday and weekend admissions.
Conclusion
Weekend admissions of myxedema coma patients have a higher risk of inpatient mortality, which has increased from 2016 to 2020. Further studies are needed to identify factors contributing to this disparity and to confirm the findings of increasing mortality related to myxedema.
Needle-shaped beam optical coherence tomography angiography visualizing cortical blood perfusion after photothrombotic stroke in vivo
Authors: Huang Yong , Guo Xiangyu , Zhao Jingjing , Sun Liqun , Gupta Varun , Du Lin , Sharma Komal , Vleck Aidan Van , Liang Kaitlyn , Cao Liangcai , Kong Lingjie , Yang Yuanmu , Zerda Adam de la , Jin Guofan 
Published: June 2023

Abstract
Optical imaging techniques provide low-cost, non-radiative images with high spatiotemporal resolution, making them advantageous for long-term dynamic observation of blood perfusion in stroke research and other brain studies compared to non-optical methods. However, high-resolution imaging in optical microscopy fundamentally requires a tight optical focus, and thus a limited depth of field (DOF). Consequently, large-scale, non-stitched, high-resolution images of curved surfaces, like brains, are difficult to acquire without z-axis scanning. To overcome this limitation, we developed a needle-shaped beam optical coherence tomography angiography (NB-OCTA) system, and for the first time, achieved a volumetric resolution of less than 8 µm in a non-stitched volume space of 6.4 mm × 4 mm × 620 µm in vivo. This system captures the distribution of blood vessels at 3.4-times larger depths than normal OCTA equipped with a Gaussian beam (GB-OCTA). We then employed NB-OCTA to perform long-term observation of cortical blood perfusion after stroke, and quantitatively analyzed the vessel area density (VAD) and the diameters of representative vessels in different regions over 10 days, revealing different spatiotemporal dynamics in the acute, sub-acute and chronic phase of post-ischemic revascularization. Benefiting from our NB-OCTA, we revealed that the recovery process is not only the result of spontaneous reperfusion, but also the formation of new vessels. This study provides visual and mechanistic insights into strokes and helps to deepen our understanding of the spontaneous response of brain after stroke.