bluuebunny/crossref_metadata_2025_split huggingface dataset:
Anti-Candida sp Activity of Astronium urundeuva derivatives Free and Loaded into a Nanostructured Lipid System
Authors: Bonifácio Bruna Vidal , Carvalho Flávio Alexandre , Mota Luiza de Oliveira , Silva Patrícia Bento da , de Souza Leonardo Perez , Vilegas Wagner , Chorilli Marlus , Santos André Gonzaga dos , Bauab Taís Maria 
Published: July 2023

Abstract
Infections caused by Candida strains increased, as well as strains resistant to the most conventional antimicrobials. Thus, the search for new natural sources of antimicrobials without adverse effects also increased. In this case, Astronium urundeuva Engl., a medicinal plant with several pharmacological activities, including antifungal activity, emerges as a source of antifungal compounds. Here, we evaluated the antifungal activity of A. urundeuvaand its derivatives free or loaded into a nanostructured lipid system, and combination studies against Candidastrains. A. urundeuva components purification and identification were performed through chromatographic and spectroscopic techniques, respectively. The major components identified were gallic acid (1), methyl gallate (2), ethyl gallate (3), and 1,2,3,4,6-penta-O-galoyl-β-D-glucose (PGG) (4). A. urundeuva extract, its aqueous fraction, and PGG (4) exhibited the same MIC values between 0.2 - 62.5 µg/mL against Candida strains being classified as significant activity, while the amphotericin B MIC was 0.1 - 1.2 µg/mL. Combination assay demonstrated that may occur synergism (0.37) between gallic acid (1) x methyl gallate (2). Thus, the results demonstrated as PGG (4) much as the gallic acid (1) x methyl gallate (2) combined might be the main responsible for the antifungal activity against Candida strains, mainly C. glabrata ATCC 2001 and that the tested nanostructured system maintained the antifungal activity. Through the antifungal activity of the extract and purified compounds, the extract may be employed in the development of antifungal herbal medicine, because its purification process is simple and greener than pure compounds.
Advancements in the Research of Modified Hydroxyapatite Materials for Bone Repair : a Scoping Review
Authors: Huang Leiyun , Bai Zhenglin , Fu Guangjie , Meng Zengdong 
Published: July 2023

Abstract
Modified hydroxyapatite (mHA) materials have shown efficacy in bone defect repair, exhibiting excellent properties such as biocompatibility, mechanical performance, antibacterial activity, and degradability. This article aims to consolidate existing research on the various aspects of mHA materials, discussing their superior performance and the fundamental principles of osteoinduction. Additionally, it explores the potential of these materials to facilitate the exploration of novel bone repair materials in the future. The primary objective of this study is to provide new insights into optimizing the functionality and structure of bone repair materials, as well as enhancing their biocompatibility, mechanical properties, antibacterial capabilities, and degradability. A systematic search was conducted in PubMed and Web of Science databases for relevant articles on modified hydroxyapatite materials. A total of 601 articles were retrieved, 461 articles with poor correlation with the purpose of the study, obsolete content, repetition and stage of the same author were excluded, and 140 articles that met the criteria were reviewed. The critical analysis and synthesis were based on the examination of in vitro and in vivo experimental results, and theoretical foundations of mHA materials. The discussion section addresses the current challenges and identifies future directions in this field.
Infection of Omicron BA.5.2 in patients provides broad immune responses against SARS-CoV-2
Authors: Li Le , Feng Tang , Liu Jiyan , Si Longlong 
Published: July 2023

Abstract
The host immunity induced by wide spread of Omicron variants and its influence on emerging SARS-CoV-2 variants are attracting broad attention. Here we report that the natural infection with Omicron BA.5.2 induce broad cross-reactive immunity against SARS-CoV-2 variants, which could reduce the risk of next wave of SARS-CoV-2 infection.
Enhancing Properties of Thermoplastic Starch/Natural Rubber Blends through the Synergistic Combination of PEG and Modified Natural Rubber
Authors: Songtipya Ladawan , Kalkornsurapranee Ekwipoo , Songtipya Ponusa , Sengsuk Theerarat , Promsung Rawiporn , Chuaybamrung Arthittaya , Johns Jobish 
Published: July 2023

Abstract
This study focuses on enhancing the mechanical and thermal properties of thermoplastic starch (TPS) and natural rubber (NR) blends through the incorporation of polyethylene glycol (PEG 2,000) and various types of modified natural rubber such as epoxidized natural rubber (ENR), poly(methyl methacrylate) grafted natural rubber (NR-g-PMMA), and poly(butyl methacrylate) grafted natural rubber (NR-g-PBMA). The influence of TPS/NR blend ratios, PEG content, and type of modified NR on the properties of the blends was investigated along with their water absorption and bio-degradation. The increase in ductile properties of the TPS/NR blends can be achieved by increasing the NR content. Among the series of TPS/NR blends, the highest toughness (1,630 MJ/m3) can be observed when the blend was formulated from 1.0 wt% of PEG and 10.0 wt% of ENR. The water absorption of TPS/NR blends was found to be lower than the TPS sample, but still exhibited a high water absorption rate compared to the other conventional polymers. The bio-degradation test confirmed the bio-degradation capability of TPS/NR blends according to ASTM 5988-12, and the result revealed that more than 80% of the tested samples can be bio-degraded in soil within 90 days.
Optimization of a new EAE induction strategy by subcutaneous administration of a Eukaryotic RpfE- MOG(35-55) Expressing Plasmid
Authors: Ziaei Maryam , Aliomrani Mehdi , Javdani Moosa , Shakhsi-Niaei Mostafa 
Published: July 2023

Abstract
Multiple Sclerosis (MS) is considered as the common autoimmune inflammatory disease; while, Experimental Autoimmune Encephalomyelitis (EAE) is the most recognized common animal model of the human MS disease. The present study aimed at introducing a new and simple method for induction of chronic EAE in C57BL/6J mice by using a eukaryotic vector. In this method, Resuscitation-promoting factor-E (RpfE) as an adjuvant, and Myeline oligodendrocyte glycoprotein (MOG(35−55)), as neuro-antigen were cloned into pcDNA3.1(+) IRES GFP. Thereafter, the mice received two doses of plasmids in the 1st and 7th days of experiment as follows: group A, 16–16 µg; group B, 32 − 16 µg; group C, 32–32 µg; group D, 64–64 µg and group E was the control group. The clinical scores during 9 weeks were documented before they were euthanized. There were not only significant differences in the clinical symptoms initiation and severity amongst groups but also in the time of showing highest inflammatory responses, which was about two weeks after clinical symptoms onset. Histopathological results revealed different degrees of demyelination as well as CD4+, CD8+, and CD20+ cell infiltration into the CNS tissues. High doses of the recombinant plasmid (64–64 µg) led to higher CD4+ cell accumulation, lesser demyelination, and delayed EAE induction (week 5); while, lower doses (up to 32–32 µg) resulted in higher CD8+ cells penetration, greater demyelination, and earlier EAE initiation (week four). To sum up, different doses of this eukaryotic plasmid is suggested as a simpler and about 3-times cheaper technique for induction of different pathological features of EAE model in mice.
Programmable Mixed-Signal Circuits
Authors: Tappertzhofen Stefan 
Published: July 2023

Abstract
A novel concept for programmable mixed-signal circuits is presented based on programmable transmission gates. For implementation, memristively switching devices are suggested as the most promising candidates for realization of fast and small-footprint signal routing switches with small resistance and capacity. As a proof-of-concept, LT Spice simulations of digital and analogue example circuits implemented by the new concept are demonstrated. It is discussed how important design parameters can be tuned in the circuity. Compared to competing technologies such as Field Programmable Analogue Arrays or Application-Specific Integrated Circuits, the presented concept allows for development of ultra-flexible, reconfigurable, and cheap embedded mixed-signal circuits for applications where only limited space is available or high bandwidth is required.
Spatio-temporal Simulation and Statistical Analysis of Soil Total Nitrogen in Alpine Meadow of Gannan
Authors: 张 锐祺 , Zhang Meiling , Jia Xiaonan 
Published: July 2023

Abstract

To determine the temporal and spatial characteristics of soil nitrogen in the alpine meadow of Gannan, China, the contents of soil organic nitrogen, mineral nitrogen and soil total nitrogen in Gannan alpine meadow from 1971 to 2020 were simulated based on the localized CENTURY model, on this basis, the spatial and temporal distribution of soil nitrogen of the study area was visualized. The influence of various environmental factors on soil nitrogen in the alpine meadow of Gannan was analyzed. The results show that from 1971 to 2020, the soil organic nitrogen in the study area has moderate spatial variability. Active soil organic nitrogen pool and chronic soil organic nitrogen pool accounted for 11.2% and 39.8% of total soil organic nitrogen, respectively. The inert soil organic nitrogen pool accounts for 49.0% of the total organic nitrogen in the soil. In spatial distribution, the total organic nitrogen and total mineral nitrogen in the alpine meadow of Gannan showed a decreasing trend from west to east, and the total mineral nitrogen content in the soil decreased significantly with the increase of soil depth. Correlation analysis of soil nitrogen and environmental factors and structural equation model showed that precipitation, elevation, soil organic matter content and silt content had positive correlation with soil organic nitrogen and mineral nitrogen contents. The effects of sand content and temperature on soil organic nitrogen and mineral nitrogen contents were negatively correlated.
Gas cavities in the chest wall, a report of Clostridium perfringens infection
Authors: Li Kun , Huang Xiaohan , Gu Silan , Cai Hongliu , Huang Lingtong 
Published: July 2023

Abstract
We reported a case of Clostridium perfringens infection with Gas activities in the Chest wall. Unlike the widely reported of gas cavity in liver, gas cavities in the chest wall caused by Clostridium perfringens infection is rare. Extrahepatic gas cavities indicate bloodstream infection and tissue invasion of Clostridium perfringens which can be an indicator of poor disease outcome.
Effects of multiple cations and sintering temperature on microstructure and dielectric properties in Na1/2Ln1/2Cu3Ti4O12 (Ln = Sm and Eu) ceramic materials
Authors: Yuan Long-Fei , Zhang Ting , Han Dan-Dan 
Published: July 2023

Abstract
Na1/2Eu1/2Cu3Ti4O12 and Na1/2Sm1/2Cu3Ti4O12 dielectric ceramics were synthesized at different sintering temperatures (950, 975 and 1000 oC) by a solid-state reaction method. Phase structure, cation valence state, and dielectric properties of all sintered ceramics were systematically investigated. When the preparation temperature was changed, the Cu+ ion concentration of (Na+, Eu3+) co-doped ceramics changed faster than that of (Na+, Sm3+) co-doped ceramics. Abnormally high dielectric permittivity ~3.17 Í 104 were accomplished of Na1/2Sm1/2Cu3Ti4O12 ceramic at a sintering temperature 950 oC. However, Na1/2Eu1/2Cu3Ti4O12 and Na1/2Sm1/2Cu3Ti4O12 prepared in high sintering temperature (1000 oC) exhibited a good dielectric stability and low loss tangent (0.061 and 0.102 at 10 Hz, respectively). It was demonstrated that an increasing number of charge carriers induced by the increase of sintering temperature could lead to a competitive coexistence of two polarization mechanisms (surface barrier layer capacitor and internal barrier layer capacitor), further changing the dielectric properties of CaCu3Ti4O12-based ceramics.
Bushen Tiaochong Recipe induces resistance to apoptosis in oocytes by activating the JNK pathway, inhibiting mitochondrial autophagy and promoting mitochondrial fusion
Authors: Li Shuang , Zhang Yin-Feng , Han Ying , Wang Xin-Yan , Zhang Yun-Shan , Luo Hai-Ning 
Published: July 2023

Abstract
Background: The mechanisms underlying ovarian function regulation and decline have been a hot topic of research. Based on the traditional Chinese medicine concept of "pattern differentiation-physiologic system-pathway analysis," a systematic characterization method can be used to investigate these issues. In this study, we used microproteomics techniques to compare the differential protein expression in oocytes of diminished ovarian reserve (DOR) mice, and drug-treated mice, and to comprehensively analyze the regulatory effects of Bushen Tiaochong Recipe (BSTCR) on the level and functional effects of protein expression in DOR mice.
Methods: There were 10 mice in each of the groups, which included the blank group, the model group, the Western medicine group, and the traditional Chinese medicine group. After 6 weeks of gavage, superovulation was induced in the mice, and seven eggs was collected from each mouse for testing. We analyzed and compared the differentially expressed proteins in oocytes of DOR mice and drug-treated mice.
Results: Using microproteomic technology, we found that the expression of the apoptosis-related proteins BAX and MK09 was significantly downregulated after treatment with BSTCR, and this may inhibit oocyte apoptosis by affecting the JNK-c-Jun pathway. The core proteins UBP30 and MOB1B were significantly downregulated, inhibiting mitophagy to exert antiapoptotic and cell growth effects, which are potential targets of BSTCR.
Conclusion: Overall, we found that BSTCR regulates the expression of MFN1, promotes mitochondrial fusion, and enhances mitochondrial function. Furthermore, BSTCR exerts anti-apoptotic and growth-promoting effects on oocytes by inhibiting mitochondrial autophagy and promoting mitochondrial fusion.
Myxobolus longirostris sp. n. (Myxozoa: Myxobolidae) infecting the brain of Chinese longsnout catfish Leiocassis longirostris (Günther, 1864) in China
Authors: Zhao Xiaojing , Xu Liwen , Wei Qiang , Yin Qi , Yang Yijing , Zhang Jinyong , Xiang Jianguo , Yu Jianbo , Li Deliang , Liu Xinhua 
Published: July 2023

Abstract
During an investigation of Myxobolus diversity in the Chinese longsnout catfish Leiocassis longirostris (Günther, 1864), a new species infecting the intracranial epidermis of the host was discovered. Upon opening the cranial cavity, several round whitish plasmodia measuring 0.55–0.80 mm in diameter were observed. Fresh spores (n = 50) were pyriform in the frontal view and fusiform in the sutural view, with a length of 15.4 ± 0.6 (13.9–16.5) µm, width of 9.1 ± 0.4 (8.3–9.8) µm, and thickness of 7.0 ± 0.4 (6.3–7.9) µm. The spores had smooth shell surfaces and transparent membrane sheaths in the posterior. No folds, intercapsular appendix, and caudal appendages were observed. Two equal polar capsules were pyriform and measured 7.5 ± 0.5 (6.7–8.7) µm in length and 3.2 ± 0.3 (2.5–3.6) µm in width. The polar filaments were coiled with five to six turns and perpendicular to polar capsule length. A BLAST search indicated Myxobolus longirostris sp. n. was closely related to four Myxobolus species (with sequences similarities ranging from 91.8–97.1%) found in different organs of yellow catfish Tachysurus fulvidraco, rather than the Leiocassis-infecting species M. branchiola (with 90.5% sequence similarity). Phylogenetic analysis showed that Myxobolus longirostris sp. n. clustered with M. jianlinensis and M. voremkhai within the Siluriformes-clade with highly supported values. This suggests that the host may play an important role in the evolution of Myxobolus species. Based on the morphological, ecological, and molecular differences observed between the newly discovered species and other available Myxobolus species, Myxobolus longirostris sp. n., is proposed and described in this study.
Influence of Samarium Doping on Structural, thermoelectrical properties of Nano Crystalline Cobalt Ferrite
Authors: Siddeshwar M. , Boda Nehru , Chittury Kanchana Latha , S B Shanti 
Published: July 2023

Abstract

Samarium substituted Cobalt ferrite nanoparticles with chemical formula CoSmXFe2-XO4 (where X=0.0, 0.010, 0.030, 0.050, 0.070 0.090 and 0.10) were synthesized via citrate-gel auto combustion method. Followed by annealing at 500 °C for 4 h in air. The physical properties of the sample were detected through x-ray diffraction, Fourier transformation infrared spectroscopy transmission electron microscopy, scanning electron microscopy, thermo electric power measurements, and dielectric measurement techniques. X-ray diffraction (XRD) analysis showed single-phase cubic spinel structure of the ferrites and the values of lattice constant (a) and x-ray density (dx) increased with the increase of Sm3+ content. The surface morphology of the samples was observed by scanning electron microscopy (SEM). Thermoelectric power studies of prepared samples were carried out from room temperature to well beyond Transition temperature (K) with a differential method. The seebeck coefficient variation with temperature was studied and it shows the prepared samples are n-type semiconducting nature. The transition temperatures of the prepared samples were also observed from thermoelectric power studies measurements. The dielectric parameters like real part of dielectric constant(εʹ), imaginary part dielectric constant (εʺ), Loss tangent(Tanδ), AC Conductivity, of the samples were studied as a function of frequency in the range of 50Hz to5MHz at room temperature using Agilent E4980 were studied. The observed result can be explained on the basis of conduction mechanism.
Impact of care disruption during Covid-19 on the Mental and Physical Well-being of cancer survivors: a nationally representative survey-based study
Authors: Elkefi Safa , Matthews Alicia K. 
Published: July 2023

Abstract
Purpose
Our study explores the care disruption in cancer care among the different demographic subgroups. It also investigates the impact of care disruption on cancer survivors’ mental and physical well-being.
Methods
This study utilized the HINTS-SEER data (Health Information Trends Survey for Surveillance Epidemiology and End Results). Missing data was addressed using the K-nearest-neighbor algorithm. Descriptive statistics were conducted to understand the care disruption perceptions. Partial least squares structural equation models were employed for data analysis.
Results
Most of the cancer survivors thought that Covid-19 impacted their cancer treatment or cancer follow-up medical appointments (69.45%), their routine cancer screening or preventative care (60.70%), and their cancer treatment plans (73.58%). Only 28.03% of them thought the pandemic changed their interactions with their healthcare providers (HCPs). Elderly patients were more likely to report visits and appointment disruption. The interactions with HCPs showed significant correlations with the demographics. People who thought their contact with their doctors changed during Covid-19 are more likely to be older adults, white, or with higher income. Mental and physical well-being were negatively impacted by the changes in cancer treatment and visits but not by changes in the interactions with HCPs.
Conclusions & implications for cancer survivors
These findings underscore the importance of maintaining regular care for cancer survivors. They also emphasize the need for targeted interventions to address disparities in access to cancer treatment and follow-up care among the different demographic groups. Maintaining communication between survivors and HCPs may have played an important role in mitigating care disruption.
SUMOylation in astrocytes induces changes in the proteome of the derived small extracellular vesicles which change protein synthesis and dendrite morphology in target neurons
Authors: Fernandez Anllely , Corvalan Katherine , Santis Octavia , Méndez-Ruette Maxs , Caviedes Ariel , Pizarro Matias , Gomez Maria-Teresa , Batiz Luis Federico , Landgraf Peter , Kahne Thilo , Rojas-Fernandez Alejandro , Wyneken Ursula 
Published: July 2023

Abstract
Emerging evidence highlights the relevance of the protein post-translational modification by SUMO (Small Ubiquitin-like Modifier) in the central nervous system for modulating cognition and plasticity in health and disease. In these processes, astrocyte-to-neuron crosstalk mediated by extracellular vesicles (EVs) plays a yet poorly understood role. Small EVs (sEVs), including microvesicles and exosomes, contain a molecular cargo of lipids, proteins, and nucleic acids that define their biological effect on target cells. Here, we investigated whether SUMOylation globally impacts the sEV protein cargo. For this, sEVs were isolated from primary cultures of astrocytes by ultracentrifugation or by the use of a commercial sEV isolation kit. SUMO levels were regulated: 1) via plasmids that over-express SUMO, or 2) via experimental conditions that increase SUMOylation, i.e., by using the stress hormone corticosterone, or 3) via the SUMOylation inhibitor 2-D08 (2′,3′,4′-trihydroxy-flavone, 2-(2,3,4-Trihydroxyphenyl)-4H-1-Benzopyran-4-one). Corticosterone and 2-D08 had opposing effects on the number of sEVs and on their protein cargo. Proteomic analysis showed that increased SUMOylation in corticosterone-treated or plasmid-transfected astrocytes increased the presence of proteins related to cell division, transcription, and protein translation in the derived sEVs. When sEVs derived from corticosterone-treated astrocytes were transferred to neurons to assess their impact on protein synthesis using the fluorescence non-canonical amino acid tagging assay (FUNCAT), we detected an increase in protein synthesis, while sEVs from 2-D08-treated astrocytes had no effect. Our results show that SUMO conjugation plays an important role in the modulation of the proteome of astrocyte-derived sEVs with a potential functional impact on neurons.
Homoharringtonine enhances cytarabine induced apoptosis in acute myeloid leukemia by regulating p38 MAPK/H2AX/Mcl-1 axis
Authors: Qiu Yang , Bai Lu , Zhao Haosen , Mei Xifan 
Published: July 2023

Abstract
Acute myeloid leukemia (AML) is a fatal disease of hematopoietic malignancies and treated with the conventional combination of cytarabine (Ara-C) and daunorubicin (Dau). Homoharringtonine (HHT) is a natural alkaloid obtained from Cephalotaxus species which was used in China for AML combination treatment. Clinically it has been reported HHT plus Ara-C was equally effective as Dau plus Ara-C in some types of AML patients with less toxic effect. We found HHT downregulates Mcl-1, phosphorylates H2AX and induces apoptosis by activating p38 MAPK. Inactivation of p38 through inhibitors and siRNA blocks apoptosis, H2AX phosphorylation and Mcl-1 reduction. HHT enhances Ara-C activation of p38 MAPK signaling pathway, overcoming Ara-C tolerance to cell apoptosis by regulating the p38/H2AX/Mcl-1 axis. 1:4 (M/M) is the optimal ratio of HHT and Ara-C for AML cell synergistic lethality. HHT synergistically induces apoptosis with Ara-C in vitro and prolongs survival of THP-1 xenografts. We provide a new mechanism for AML treatment by regulating p38 MAPK/H2AX/Mcl-1 axis to improve cytarabine therapy.