bluuebunny/crossref_metadata_2025_split huggingface dataset:
A new predictor of the optimal pulmonary artery reconstruction method during the arterial switch operation
Authors: Hongu Hisayuki , Oda Shinichiro , Maeda Yoshinobu , Asada Satoshi , Fujita Shuhei , Yamashita Eijiro , Nagase Takashi , Yamagishi Masaaki 
Published: May 2023

Abstract

OBJECTIVES
The aim of this study was to more accurately predict the optimal pulmonary artery (PA) reconstruction procedure (Lecompte manoeuvre or original Jatene procedure) during the arterial switch operation, we focused on the horizontal sectioning (HS) angle between the left hilum PA and the great arteries using preoperative computed tomography imaging.

METHODS
We defined the HS angle α (β) as the angle between the tangential line from the posterior (anterior) wall of the left PA at the hilum to the left anterior (right posterior) surface of the main PA and the tangential line from the left surface of the ascending aorta to the left anterior (right posterior) surface of the main PA. We identified 14 consecutive patients diagnosed with transposition of the great arteries or transposition of the great artery-type double-outlet right ventricle who underwent preoperative computed tomography imaging. The original Jatene or Lecompte procedure was used for 9 (OJ group) and 5 (L group) patients. Relationships of the great arteries of the OJ and L groups were side by side in 8 and 2 patients, oblique in 1 and 1 patient and anteroposterior in 0 and 2 patients, respectively.

RESULTS
In the OJ group, β was greater than α in all patients. The median α/β value was 0.618. In group L, α was greater than β in all patients. The median α/β was 1.307. Left PA stenosis caused by stretching was not observed in the L group. Coronary obstruction was not identified in the OJ group. Left PA stenosis behind the neo-ascending aorta was observed in 1 patient in the OJ group and required reoperation.

CONCLUSIONS
The HS angle may be a useful predictor of optimal intraoperative PA reconstruction during arterial switch operation, especially for side-by-side or oblique relationships.
Barriers and facilitators of academia-government collaboration in the context of the COVID-19 pandemic in Colombia: a qualitative study
Authors: Rodriguez-Villamizar Laura Andrea , Linares García Johanna , Ruiz-Rodriguez Myriam 
Published: May 2023

Abstract
The COVID-19 pandemic highlighted the importance of strengthening collaborations between academia and government. The development and maintenance of these collaborative relationships is a complex and dynamic process, particularly during public health emergencies. This study was aimed at identifying and analysing factors that acted as barriers and facilitators in the collaboration process between academia and government during the COVID-19 pandemic in the five largest cities in Colombia. The study used a qualitative approach based on the systematization of experiences. A total of 25 semi-structured interviews were conducted with local actors from government and academia during 2021. Participants identified a variety of situations that involved individual, institutional and relational factors that acted as barriers and facilitators, and which have been previously reported in other countries and contexts not related to pandemics. Based on participant reports, two additional factors emerged, one corresponding to situations related to the pandemic management process itself and another related to structural or systemic conditions that involved government processes and the Colombian health system. Despite the challenges posed by the pandemic, the health emergency brought about shared feelings of local commitment and a willingness to work through interdisciplinary teams to address the pandemic with the least adverse effects on the community. Other facilitators of the collaborative process that were recognized were the importance of timely access to data and transparent analyses, as well as government decisions being informed by the perspectives of academics. The main barriers identified by both actors were excessive centralization of the pandemic’s management and the need for rapid decision-making processes under high levels of uncertainty. In addition, the fragmentation of services in the health system posed a barrier to the interventions that were suggested by the collaborative work. Our results suggest that government–academia collaborations should be implemented as ongoing participatory processes integrating various sectors, actors and disciplines.
The m6A reader MhYTP2 regulates the stability of its target mRNAs contributing to low nitrogen tolerance in apple (Malus domestica)
Authors: Guo Tianli , Yang Zehua , Bao Ru , Fu Xiaomin , Wang Na , Liu Changhai , Ma Fengwang 
Published: May 2023

AbstractStudies have shown that the m6A reader primarily affects genes expression by participating in the regulation of mRNA localization, splicing, degradation, translation, and other metabolic processes. Previously, we discovered that the apple (Malus domestica) m6A reader MhYTP2 bound with and destabilized m6A-modified MdMLO19 mRNA. In addition, it enhanced the translation efficiency of m6A-modified mRNA of MdGDH1L, encoding a glutamate dehydrogenase, which confers resistance to powdery mildew. In this study, we report the function of MhYTP2 in the regulation of resistance to low nitrogen (N). The overexpression of MhYTP2 enhances the resistance of apple to low N. We show that MhYTP2 binds with and stabilizes the mRNAs of MdALN, which participates in the allantoin catabolic process and cellular response to N starvation in apple; MdPIDL, which participates in root hair elongation; MdTTG1, which is involved in the differentiation process of trichomes; and MdATG8A, which is a core participant in the regulation of autophagy. In addition, MhYTP2 accelerates the degradation of MdRHD3 mRNA, which regulates root development. RNA immunoprecipitation-seq and electrophoretic mobility shift assays show that the mRNAs of MdALN, MdATG8A, MdPIDL, MdTTG1, and MdRHD3 are the direct targets of MhYTP2. Overexpressing or knocking down the above genes in MhYTP2 overexpressing plants dismisses the function of MhYTP2 under low N, suggesting the role of MhYTP2 is dependent on those genes. Together, these results demonstrate that MhYTP2 enhances the resistance of apple to N deficiency by affecting the stability of the bound mRNAs.
On the null controllability of integer order integro-differential equations
Authors: Zhou Xiuxiang , Cheng Lijuan , Wang Xin 
Published: May 2023

Abstract
This paper is addressed to the study of the null controllability for integer order integro-differential equations. Unlike the known results for partial differential equations, we need to consider the equation involving a $\beta -$power of the Laplace operator $(-\varDelta )^\beta $ and an integral term. The key point is to construct a suitable state space of the controlled system at the final time. We first discuss a class of hyperbolic integro-differential equation. We prove that the controlled system is null controllable by an Ingham-type estimate. Also, the controllability time is given. On the other hand, by reduction to absurdity, we deduce that the null controllability property fails for a class of parabolic integro-differential equation with $\beta \in \mathbb{N}^+$.
Subcutaneous immunisation with zymosan generates mucosal IgA-eliciting memory and protects mice from heterologous influenza virus infection
Authors: Nihei Yoshihito , Higashiyama Mizuki , Miyauchi Kosuke , Haniuda Kei , Suzuki Yusuke , Kubo Masato , Kitamura Daisuke 
Published: May 2023

Abstract
Immunoglobulin A (IgA) is the most abundant isotype of antibodies and provides a first line of defense at the mucosa against pathogens invading the host. It has been widely accepted that the mucosal IgA response provided by vaccination requires mucosal inoculation, and intranasal inoculation has been proposed for vaccines against influenza virus. Considering the difficulty of intranasal vaccination in infants or elderly people, however, parenteral vaccination that provides the mucosal IgA response is desirable. Here, we demonstrate that subcutaneous immunisation with zymosan, a yeast cell wall constituent known to be recognised by Dectin-1 and TLR2, potentiates the production of antigen-specific IgA antibodies in the sera and airway mucosa upon intranasal antigen challenge. We confirmed that the antigen-specific IgA-secreting cells accumulated in the lung and nasal-associated lymphoid tissues after the antigen challenge. Such an adjuvant effect of zymosan in the primary immunisation for the IgA response depended on Dectin-1 signalling, but not on TLR2. The IgA response to the antigen challenge required both antigen-specific memory B and T cells, and the generation of memory T cells, but not memory B cells, depended on zymosan as an adjuvant. Finally, we demonstrated that subcutaneous inoculation of inactivated influenza virus with zymosan, but not with alum, mostly protected the mice from infection with a lethal dose of a heterologous virus strain. These data suggest that zymosan is a possible adjuvant for parenteral immunisation that generates memory IgA responses to respiratory viruses such as influenza virus.
57 Relationship between Proportion ofBos indicus Genetics of Dams and Calf Performance from a 31-Year Multibreed Herd Dataset
Authors: Martins Thiago , Rocha Cecilia Constantino C , Driver Joseph Danny D , Rae Owen , Aguirre Elzo Mauricio A , Mateescu Raluca G , Portela Santos José Eduardo E , Binelli Mario 
Published: May 2023

Abstract
This is a 31-year retrospective cohort study aiming to evaluate the relationship between the proportion of B. indicus genetics of crossbred dams and the weaning weight of their calves. Our hypothesis was that crossbred dams with intermediate degree of B. indicus genetics wean heavier calves than those with low-to-high degree of B. indicus genetics at the subtropical condition. The cow-calf pairs (n = 4,901) were in six evenly distributed breed groups according to the proportion of Brahman: G0–19%, G21–34%, 38%, G41–59%; G63–78% and G81–100%. Dams reared contemporaneously were undergone to controlled breeding seasons where the six breed groups of dams and sires were reciprocally mated. Non-adjusted and 205d-adjusted weaning weight of calves were considered as dependent variables and analyzed using MIXED procedure of SAS 9.4. The following independent variables were included in the model as fixed effects: parity category order (primiparous, secundiparous and pluriparous), calving period (0-30d, 31-60d, 61-90d and 91-120d), gender of calf (male or female) and breed group. Year was included as a random effect. All possible interactions were tested and only those with P &lt; 0.20 remained in the final model. Most of all, for the non-adjusted weaning weight, the breed group effect (P &lt; 0.001) varied according to the calving period. Dams calving after 30d of calving season weaned the lightest calves. However, the magnitude of such losses was variable within breed groups. Calves from G0-19% and G81-100% of dams were the most and least impacted by calving period, respectively. The difference on weaning weight between calves born at 0-30d and 91-120d was 60kg for G0-19% of dams and 30kg for G81-100% of dams. For every other breed group, this difference ranged from 45kg to 55kg. In spite of such interaction effect, calves from G0-19% and G81-100% of dams were lighter (230.4 and 225.0 kg, respectively; SEM: 3.4) than any other breed group (238.3 to 242.5 kg). For the 205d-adjusted weaning weight, there was a breed group effect (P &lt; 0.001), regardless of calving period. Lighter calves were still weaned from G0-19% and G81-100% of dams (214.9 and 206.5 kg, respectively, SEM: 2.8) compared with any other breed group (223.4 to 227.6 kg). Thus, crossbred dams with intermediate degree of B. indicus genetics weaned the heaviest calves, probably as result of outbreeding enhancement provided by the adaptability of B. indicus to the tropics and productivity of B. taurus.
58 Reproductive Performance of Beef Cow-Calf Operations in VA with a Fall or Spring Breeding Season
Authors: Goncherenko Guillermo , Pancini Stefania , Dias Nicholas W , Craun Hannah , Vidlund Trinity , Klonicke Zachary , Redifer Tracey , Currin John , Clark Sherrie , Stewart Jamie L , Mercadante Vitor R G 
Published: May 2023

Abstract
Establishing two breeding seasons (Fall and Spring) is a common practice in the southeast USA, including VA. We performed a retrospective analysis of data from 11 years, 22 breeding seasons and 14 locations in VA to compare reproductive performance between Fall and Spring breeding seasons. A total of 30,191 breeding records (24,032 for cows and 6,259 for heifers) from the VA Department of Corrections beef cattle operations were analyzed. Every year, each cow and heifer were enrolled in estrous synchronization protocols and received fixed-time artificial insemination (TAI) and were then exposed to natural service bulls for an approximately 65-day breeding season. Fall breeding occurred from November until January, and Spring breeding from March until May. Body condition score (BCS; 1 to 9, scale) was determined at the initiation of the TAI protocol. Estrus detection before TAI was performed by scoring an estrus detection aid prior to insemination. Pregnancy rate was determined by ultrasonography between days 55-65 post-TAI and 40-50 after the end of the breeding season. Data were analyzed using the SAS GLIMMIX procedure for binary data and MIXED procedure for continuous data. Average BCS was greater (P &lt; 0.001) for Fall cattle than Spring cattle (5.7 ± 0.02 and 5.1 ± 0.02, respectively); however, days postpartum at TAI was similar between Fall and Spring (P = 0.645; 77.8 and 76.2 ± 0.5 days, respectively). Pregnancy rate to TAI differed (P &lt; 0.0001) between Fall and Spring, 53.0 ± 0.03% and 48.6 ± 0.05%, respectively. Interestingly, estrus expression was greater (P &lt; 0.001) for Spring than Fall (58.4 ± 0.05% and 50.4 ± 0.06%, respectively) and also was greater for cows that became pregnant to TAI compared with cows that failed to become pregnant to TAI (P &lt; 0.001; 61.7 ± 0.03% and 46.9 ± 0.04%, respectively). End of breeding season pregnancy rate differed between season and was greater for Fall than Spring (P &lt; 0.001; 90.7 ± 0.04% and 87.9 ± 0.04%, respectively). Calf birth weight was similar between seasons (P = 0.478; 34.1 and 34.4 ± 0.09 kg, for Fall and Spring, respectively). However, 205-day adjusted weaning weights differed (P &lt; 0.001) between seasons with Spring calves being heavier than Fall calves (246.3 ± 0.12 kg and 227.8 ± 0.21 kg, respectively). In conclusion, for beef cow-calf operations in VA reproductive performance is enhanced for Fall breeding seasons; however, weaning weights were greater for calves born during the Spring seasons.
179 An Evaluation of Oklahoma Beef Calf Market Characteristic Trends from 2011 to 2021
Authors: Vining Timothy P , Raper Kellie C , Peel Derrell S , Robe Jeff , Freking Brian M , Zook Dana L , New Marty G , Beck Paul A 
Published: May 2023

Abstract
The Oklahoma Quality Beef Network (OQBN) is a partnership between the Oklahoma State University Extension Service and the Oklahoma Cattlemen’s Association, with the goal of providing and promoting value-added marketing opportunities for beef cattle producers. The Oklahoma Beef Network’s goal is realized through the implementation of the OQBN Vac-45 Program. The OQBN Vac-45 Program is a beef calf preconditioning program, requiring specific vaccination and management practices. Calves that fulfill program requirements receive OQBN Vac-45 Certification and may participate in an “OQBN Sanctioned Sale”. These sales occur as part of regularly scheduled livestock auction sales on specified dates at planned locations. Sale data for all calves sold at OQBN Sanctioned Sales (certified and non-certified calves) were collected each year to capture market premiums attained by OQBN Vac-45 certified calves. The objective of this study was to analyze 11 years of beef calf sale data (2011-2021), collected by extension specialists at 91 individual OQBN Vac-45 sales, which took place at 10 Oklahoma livestock auction locations. An average of 8 sales were conducted each year. A total of 149,525 calves were described using 42 market characteristics for 12 market traits (the number of assigned characteristics depended on the given trait). Market characteristics were analyzed as percentages based on the total number of calves recorded each year. Linear and quadratic regression analysis procedures, with year as the fixed effect were performed using R Statistical Software (v4.1.3; R Core Team 2022). A linear increase in the percentage of red-hided calves sold over time was observed, explained by (R2 = 0.51, P = 0.008). A Kansas State University study by McCabe et al. (2020) regarding breed trends in beef calf lots sold in video auctions from 1995 to 2018, indicated an increase in the number of calves sired by Red Angus bulls (P &lt; 0.0001). An inverse quadratic effect was observed with the percentage of over-conditioned “fleshy” calves decreasing (R2 = 0.89, P &lt; 0.05) and the number of optimally conditioned “average flesh” calves increasing (R2 =0.95. P &lt; 0.05) over time, indicating optimal calf nutritional management. Quadratic regression indicated a decreasing trend (R2 = 0.60, P = 0.01) in the percentage of calves participating in certified preconditioning programs over time, indicating that producers may perceive certified preconditioning programs as an unneeded additional cost. However, from 2011 to 2021 calves enrolled in the OQBN Vac-45 Program received an average premium of $12.55/cwt compared with non-preconditioned calves sold in the same sales. Further data are needed to evaluate the frequency and efficacy of preconditioning practices occurring without participation in certified programs.
13 Stockpiling Bahiagrass to Extend the Grazing Season and decrease Winter Supplementation Costs for South Alabama Cattle Producers
Authors: Agar Michael A , Mullenix Kim K , Vasco Carol , Justice Maggie M , Palmer Kelly , Kelley Ken , Elmore Josh , Parker Chris , Wiggins Anthony 
Published: May 2023

Abstract
Stockpiling forage is a management practice that can be used to decrease the cost of winter cow supplementation and is defined as the process of accumulating forage late in a growing season for grazing at a later date. The purpose of this study was to explore the effectiveness of bahiagrass as a potential stockpiled forage in lower Alabama by developing a better understanding of its forage accumulation, nutritive value and persistent characteristics during the stockpiling period (6 to 10 weeks). A small-plot stockpiling trial was conducted at three locations in south Alabama (Troy, Headland, and Goodway). Nine, 6.5 m x 2.0 m plots were cut to a 7.5 cm stubble height with a buffer of 0.9 m between plots at each location beginning on September 16, 2021. Nitrogen fertilization strategy was the main plot [0 N fertilizer, split N application (33.6 kg N/ha at initiation and again 30 d later), or 67.2 kg N/ha; 3 replications/treatment; N source = urea (46-0-0)]. Stockpiling period length was the split-plot (6, 8 or 10 weeks of forage accumulation; n = 9 subplots/treatment). Response variables were measured including forage mass and nutritive value at 6, 8 and 10 weeks, and nutritive value of standing forage 30 days after the stockpiling period. Data were analyzed using the PROC MIXED procedure of SAS 9.3. As the accumulation period length increased, average forage mass increased across all treatments with 1,312.6 kg DM/ha at 6 weeks, 1,270.1 kg DM/ha at 8 weeks and 1,585.9 kg DM/ha at 10 weeks. With respect to the fertilization treatments, the split N application (1,621.8 kg DM/ha) and 67.2 kg N/ha fertilization strategies (1,606.1 kg DM/ha) did not differ in forage accumulation but were greater (P &lt; 0.05) than bahiagrass not receiving N fertilizer (1,377.6 kg DM/ha). For nutritive value, nitrogen application strategy impacted bahiagrass crude protein (CP), with both the split N application (14%) and 67.2 kg N/ha (13.8%) treatments having a greater CP (P &lt; 0.05) than the 0 N fertilization approach (11.7%). Average total digestible nutrients across stockpiling periods were 62.5%. Year one results study indicate that bahiagrass may support forage production and nutritive value for cow-calf operations wanting to extend grazing into the fall and early winter months in the Southeast US. Based on year one results for forage mass measured at 6, 8 and 10 weeks, stockpiled bahiagrass could provide an estimated 45 to 55 days of grazing depending on mature cow weight, pasture stocking strategy and accumulation period length.
32 Depot-Specific Transcriptome and Adipocyte Metabolism in Intramuscular and Subcutaneous Adipose Tissue in Beef Cattle
Authors: Tegeler Alexandra P , Fiallo-Diez Jean , Michelotti Tainara , Flores Lauryn , Benitez Oscar J , Johnson Bradley J , Strieder-Barboza Clarissa 
Published: May 2023

Abstract
Intramuscular adipose tissue (IMAT; marbling) deposition is the primary determination of beef quality grade in the U.S. While finishing diets increase marbling, they also increase subcutaneous adipose tissue (SAT) accumulation, leading to excessive fattening and economic losses due to ‘waste fat’. Our objective was to investigate the IMAT and SAT-specific transcriptional profile and adipocyte function in vitro. A better understanding of the mechanisms underlying IMAT and SAT-specific characteristics will help to identify molecular and cellular molecules for developing targeted strategies to enhance marbling while limiting overall fatness in beef animals. A section of the Longissimus dorsi muscle from 9-11th ribs was collected to dissect IMAT and adjacent SAT (backfat) from six beef animals harvested at the Texas Tech University Meat Lab. The SAT and IMAT RNA was extracted and sequenced in an Illumina NovaSeq 6000 targeting a minimum of 30 million reads per sample. Transcriptome analysis showed more than 1,800 differently expressed genes (DEGs) in IMAT and SAT. There was a downregulation of genes associated with adipogenesis, lipolysis regulation, insulin sensitivity, and fatty acid metabolism (e.g., LEP, INSIG1, and PLIN4) in IMAT vs. SAT, while pathways of insulin resistance and ß-adrenergic signaling were activated. To elucidate whether the differences between IMAT and SAT transcriptional profiles were translated into changes in the adipocyte function, preadipocytes were isolated, cultured, and differentiated into adipocytes in vitro for 14 followed by functional analyses of adipogenic capacity, insulin sensitivity, and lipolytic responses. Statistical analysis was performed in GraphPad 9. Even though preadipocytes were more abundant in IMAT than in SAT (flow cytometry quantification of CD45-CD31- cell), IMAT preadipocyte proliferation rates were decreased compared with SAT. Additionally, SAT adipocytes were larger than IMAT. These findings suggest an enhancement in SAT adipogenesis/lipogenesis compared with IMAT. Metabolically, SAT adipocytes released greater glycerol concentrations compared with IMAT upon ß-adrenergic stimulation of lipolysis with isoproterenol and tended to have greater insulin-stimulated glucose uptake compared with IMAT. Altogether, these findings indicate that IMAT is unable to accumulate lipids as efficiently as SAT, and these differences may be underlined by a decreased preadipocyte hyperplasia, insulin resistance, and potential dysregulation of ß-adrenergic receptors, all of these similarly suggested by the transcriptome analysis. Our study revealed molecular and cellular differences between IMAT and SAT that may partly explain why it is common to observe increased SAT fatness and limited marbling beef cattle.