bluuebunny/crossref_metadata_2025_split huggingface dataset:
Do good carefully: The long‐term effects of low‐dose DDT exposure in early childhood on education, marriage and employment
Authors: Chang Simon , Kan Kamhon 
Published: December 2022

AbstractIt is well known that malaria has serious adverse effects on humans. Yet, little is known as to how dichlorodiphenyltrichloroethane (DDT), which is still used to control malaria, may affect human socioeconomic outcomes in the long run. Utilizing the large‐scale indoor residual spraying of low‐dose DDT in Taiwan in the 1950s, we estimated the long‐term effects of low‐dose DDT exposure in early childhood on education, marriage and employment in adulthood. Our identification hinges on the unexpected extension of DDT spraying after malaria had already been largely brought under control. We found that even at a very low dosage, DDT exposure still resulted in discernible negative effects on education and marriage. For employment, although no effect on the probability of working was detected, people exposed to more sprayings in childhood were more likely to work in the agricultural sector that typically requires less human capital.
Correcting Hargreaves‐Samani formula using geographical coordinates and rainfall over different timescales
Authors: Elagib Nadir Ahmed , Musa Ammar Ahmed 
Published: December 2022

AbstractThe United Nations Food and Agricultural Organization (FAO) approved the Hargreaves‐Samani formula (HAR‐85) as an alternative to the standard Penman‐Monteith method (FAO‐PM) for estimating grass reference evapotranspiration (ETo). With much less data demand, HAR‐85 is unequivocally useful where meteorological variables are often scarce, incomplete or unavailable. Herein, we evaluate HAR‐85 against FAO‐PM across 2.505 million km2, representing Sudan and South Sudan and encompassing wide hydroclimate domains including the Nile River. We further propose simple year‐round and seasonal adjustment models to correcting HAR‐85 across the entire study area. The models express HAR‐85's error in multiple linear regressions in terms of latitude, longitude, altitude and/or monthly rainfall. Varying data periods, including odd, even and all years, are used in the evaluation and the adjustment models development and validation processes to investigate the influence of changing data period. A suit of eight performance indicators shows dependency of the original bias of HAR‐85 on the geographical location, monthly rainfall amount, season of the year and data period. All error indicators amplify southward from the hyper‐arid region to the dry sub‐humid zone. For example, the mean bias error (MBE) ranges from −0.51 to 1.29 mm/day, respectively. Study area‐wide, HAR‐85 least represents FAO‐PM during the hottest month and the transitional month (between the wet and dry‐cool seasons) with MBE of 0.65 and 0.70 mm/day, respectively. Conversely, it represents FAO‐PM the most in the wettest month, with smallest MBE of 0.32 mm/day. Beholding this spatiotemporal trait, the final yearly and seasonal adjustment models developed herein enormously moderate the predominant overestimation of the original HAR‐85. The former model explains 46.7% of the error variance whereas 36.9% to 62.3% of the variation in the error is explainable by the latter models. These adjustment models narrow the monthly MBE among the stations from −0.71‐2.17 to −0.80‐1.20 and −0.65‐0.99 mm/day, respectively. Without undermining the accuracy, the year‐round adjustment model can still be feasibly recommended for general use across the study area.
A case of acute lymphocytic gastritis related to treatment with pembrolizumab for metastatic urothelial carcinoma
Authors: Fukiishi Yousuke , Fukuhara Hideo , Kurano Yoshitaka , Shugimoto Hiroki , Yamashita Erika , Karasima Takashi , Inoue Keiji 
Published: December 2022

IntroductionImmune checkpoint inhibitors such as programmed cell death/−ligand 1 inhibitor and cytotoxic T‐lymphocyte‐associated antigen‐4 inhibitors have been widely used for various advanced malignancies. The mechanism of action for these inhibitors is the improvement of antitumor immunity via T‐cell modulation. On the contrary, immune‐related adverse events such as autoimmune colitis might arise in association with T‐cell activation. Upper gastrointestinal adverse events related to pembrolizumab have rarely been reported.Case presentationA 72‐year‐old man underwent laparoscopic radical cystectomy for muscle‐invasive bladder cancer (pT2N0M0). Multiple lymph node metastases appeared in the paraaortic region. First‐line chemotherapy comprising gemcitabine and carboplatin failed to stop disease progression. After the administration of pembrolizumab as second‐line treatment, the patient showed symptomatic gastroesophageal reflux disease. Esophagogastroduodenoscopic biopsy of the gastric body showed severe lymphoplasmacytic and neutrophilic infiltration.ConclusionWe present acute gastritis related to pembrolizumab. Early eradication therapy may be able to control immune checkpoint inhibitor‐related gastritis.
Deciphering mechanistic implications of antimicrobial and antioxidant potentials of certain new dibutyltin(IV) formulations as possible therapeutic options based on DFT and hybrid materials paradigm
Authors: Soni Komal , Saxena Sanjiv , Jain Asha 
Published: December 2022

AbstractMechanistic implications of antimicrobial and in vitro antioxidant potentials of a set of newly generated nonbridged mononuclear N,O‐orthometallated and carboxylate bridged binuclear nonorthometallated dibutyltin(IV) formulations have been investigated. Some of these formulations were screened for their antibacterial and antifungal activities against Escherichia coli and Candida albicans, respectively whereas in vitro antioxidant potential was examined by Ferric reducing antioxidant power (FRAP) assay. Nonbridged mononuclear N,O‐orthometallated dibutyltin(IV) formulations were generated by the reactions of Bu2SnCl2 with sodium salts of 2‐aminophenol/substituted 2‐aminophenol and flexible N‐protected amino acids in 1:1:1 molar ratio in refluxing dry THF. Plausible structures of these nonbridged mononuclear N,O‐orthometallated dibutyltin(IV) formulations containing flexible N‐protected amino acids have been suggested on the basis of spectroscopic and mass studies of some representative formulations. Plausible structures suggested on the basis of spectroscopic studies are corroborated by density functional theory (DFT/B3LYP method) (SPARTAN‐20) investigation of a representative dibutyltin(IV) complex and the ligands involved in it. The presence of two different classes of organic ligands in this complex provides an opportunity to study optimized topologies, bonding, distortions, optimized energy, and stability of the complex.
Proteomics analysis of meclofenamic acid‐treated small cell lung carcinoma cells revealed changes in cellular energy metabolism for cancer cell survival
Authors: Yanar Sevinc , Kasap Murat , Kanli Aylin , Akpinar Gurler , Sarihan Mehmet 
Published: December 2022

AbstractSmall cell lung carcinoma (SCLC) is a highly aggressive cancer with low survival rate. Although initial response to chemotherapy in SCLC patients is well‐rated, the treatments applied after the disease relapses are not successful. Drug resistance is accepted to be one of the main reasons for this failure. Therefore, there is an urgent need for new treatment strategies for SCLC. Meclofenamic acid, a nonsteroidal anti‐inflammatory drug, has been shown to have anticancer effects on various types of cancers via different mechanisms. The aim of this study was to investigate the alterations that meclofenamic acid caused on a SCLC cell line, DMS114 using the tools of proteomics namely two‐dimensional gel electrophoresis coupled to MALDI‐TOF/TOF and nHPLC coupled to LC‐MS/MS. Among the proteins identified by both methods, those showing significantly altered expression levels were evaluated using bioinformatics databases, PANTHER and STRING. The key altered metabolism upon meclofenamic acid treatment appeared to the cellular energy metabolism. Glycolysis was suppressed, whereas mitochondrial activity and oxidative phosphorylation were boosted. The cells underwent metabolic reprogramming to adapt into their new environment for survival. Metabolic reprogramming is known to cause drug resistance in several cancer types including SCLC. The identified differentially regulated proteins in here associated with energy metabolism hold value as the potential targets to overcome drug resistance in SCLC treatment.
IGF2BP2 enhances LincRNA01116 stability via m6A: A potential biomarker and therapeutic target for patients with pre‐eclampsia
Authors: Zhang Tingting , Tang Xiaotong , Zhu Yuanyuan , Wang Cong , Jiang Ziyan , Yang Nana , Wang Tianjun , Shu Lijun , Xu Yetao , Sun Lizhou 
Published: December 2022

AbstractPre‐eclampsia (PE) is a serious complication in pregnant women characterized by failure of placental remodeling and is one of the primary causes of changes in the placental structure and function. The aberrant expression of long noncoding RNA is associated with the occurrence and progression of PE. This study found that linc01116 expression was significantly downregulated in PE patients and was related to poor uterine spiral artery remodeling. Knockdown of linc01116 remarkably decreased the angiogenesis of trophoblast cells in vitro and in vivo. Mechanistically, IGF2BP2 regulated linc01116 RNA stability via m6A methylation. Bioinformatics and other experiments further revealed that linc01116 upregulates AAMP expression by adsorbing miR‐210‐3p in trophoblast cells. In conclusion, this study revealed the critical role of linc01116 in regulating trophoblast angiogenesis. Furthermore, the study provides new clues for detecting placental pathology in PE.
Effect of non‐covalent interactions in 2,5‐di‐Me‐pyrazine‐di‐N‐oxide ‐ methanol – Carbon nanotube electrocatalytic system
Authors: Kulakovskaya Svetlana I. , Zyubina Tatyana S. , Zyubin Alexander S. , Kulikov Alexander V. , Ryabenko Alexander G. , Zolotukhina Ekaterina V. , Dobrovolskiy Yuriy A. 
Published: December 2022

AbstractCatalytic oxidation of methanol (MeOH) in the absence of noble metals and noble metal oxides as catalysts, and the use of metal‐free materials are inexpensive and attractive process for practical use in electrocatalysis, sensors, and in direct methanol fuel cells. In previous works, it was found that the use of single‐walled (SWCNT) or multi‐walled (MWCNT) carbon nanotube paper electrodes instead of GC increases the catalytic efficiency of organic compounds oxidation in the presence of aromatic di‐N‐oxides by several times. In this work, the effect of non‐covalent interactions on the catalytic efficiency of MeOH oxidation in the presence of 2,5‐di‐Me‐pyrazine‐di‐N‐oxide (Pyr1) in 0.1 M Bu4NClO4 solution in acetonitrile at SWCNT and MWСNT paper electrodes was studied by the methods of quantum chemical modeling, Raman spectroscopy, and using electrochemical data. New factors determined the features of mechanism of MeOH oxidation on CNT electrodes and lead to an increase in the catalytic efficiency of the electrode process in comparison with the GC electrode were established.
The AP2/ERF transcription factor ORA59 regulates ethylene‐induced phytoalexin synthesis through modulation of an acyltransferase gene expression
Authors: Huang Li‐Jun , Zhang Jiayi , Lin Zeng , Yu Peiyao , Lu Mengzhu , Li Ning 
Published: December 2022

AbstractThe gaseous ethylene (ET) and the oxylipin‐derived jasmonic acid (JA) in plants jointly regulate an arsenal of pathogen responsive genes involved in defending against necrotrophic pathogens. The APETALA2 (AP2)/ETHYLENE RESPONSE FACTOR (ERF) transcription factor ORA59 is a major positive regulator of the ET/JA‐mediated defense pathway in Arabidopsis thaliana. The Arabidopsis agmatine coumaroyltransferase (AtACT) catalyzes the formation of hydroxycinnamic acid amides (HCAAs) which are effective toxic antimicrobial substances known as phytoalexins and play an important role in plant defense response. However, induction and regulation of AtACT gene expression and HCAAs synthesis in plants remain less understood. Through gene coexpression network analysis, we identified a list of GCC‐box cis‐element containing genes that were coexpressed with ORA59 under diverse biotic stress conditions and might be potential downstream targets of this AP2/ERF‐domain transcription factor. Particularly, ORA59 directly binds to AtACT gene promoter via the GCC‐boxes and activates AtACT gene expression. The ET precursor 1‐aminocyclopropane‐1‐carboxylic acid (ACC)‐treatment significantly induces AtACT gene expression. Both ORA59 and members of the class II TGA transcription factors are indispensable for ACC‐induced AtACT expression. Interestingly, the expression of AtACT is also subject to the signaling crosstalk of the salicylic acid‐ and ET/JA‐mediated defense response pathways. In addition, we found that genes of the phenylpropanoid metabolism pathway were specifically induced by Botrytis cinerea. Taking together, these evidence suggest that the ET/JA signaling pathway activate the expression of AtACT to increase antimicrobial HCAAs production through the transcription factor ORA59 in response to the infection of necrotrophic plant pathogens.
The role and mechanism of long non‐coding RNAs in homologous recombination repair of radiation‐induced DNA damage
Authors: Yu Nanxi , Qin Hongran , Zhang Fangxiao , Liu Tingting , Cao Kun , Yang Yanyong , Chen Yuanyuan , Cai Jianming 
Published: December 2022

AbstractDNA double‐strand breaks can seriously damage the genetic information that organisms depend on for survival and reproduction. Therefore, cells require a robust DNA damage response mechanism to repair the damaged DNA. Homologous recombination (HR) allows error‐free repair, which is key to maintaining genomic integrity. Long non‐coding RNAs (lncRNAs) are RNA molecules that are longer than 200 nucleotides. In recent years, a number of studies have found that lncRNAs can act as regulators of gene expression and DNA damage response mechanisms, including HR repair. Moreover, they have significant effects on the occurrence, development, invasion and metastasis of tumor cells, as well as the sensitivity of tumors to radiotherapy and chemotherapy. These studies have therefore begun to expose the great potential of lncRNAs for clinical applications. In this review, we focus on the regulatory roles of lncRNAs in HR repair.
A phase III, randomised, double‐blind, multi‐national clinical trial comparing SB12 (proposed eculizumab biosimilar) and reference eculizumab in patients with paroxysmal nocturnal haemoglobinuria
Authors: Jang Jun Ho , Gomez Roberta Demichelis , Bumbea Horia , Nogaieva Larysa , Wong Lily Lee Lee , Lim Soo Min , Kim Younsoo , Park Jihye 
Published: December 2022

AbstractTreatment of paroxysmal nocturnal haemoglobinuria (PNH) includes the monoclonal antibody eculizumab. This randomised, double‐blind, multi‐national cross‐over Phase III study in PNH patients aimed to demonstrate the equivalence of the proposed eculizumab biosimilar SB12 and reference eculizumab (Soliris, ECU). PNH patients with lactate dehydrogenase (LDH) ≥1·5× upper limit of normal were randomised into treatment sequences SB12‐ECU or ECU‐SB12. Four weekly infusions of 600 mg eculizumab were followed by fortnightly infusions of 900 mg until week 50 (ECU/SB12 cross‐over at week 26). Primary endpoints were LDH at week 26 and the time‐adjusted area under the effect curve (AUEC) of LDH over weeks 14‒26 and 40‒52. Among 46 patients (92%) who completed the study, the least squares mean (LSM) difference in LDH at week 26 (34·48; 95% confidence interval [CI] −47·66‒116·62 U/l) and geometric LSM ratio of time‐adjusted AUEC of LDH (1·08; 90% CI 0·95‒1·23) were within pre‐defined equivalence margins. Mean numbers of transfused red blood cell units, other secondary endpoints, pharmacokinetics, and pharmacodynamics were comparable. No patients developed anti‐drug antibodies. Treatment‐emergent adverse events were reported in 72% and 68% of patients in the SB12 and ECU treatment groups, respectively. The results demonstrate equivalence of SB12 to ECU and support SB12‐use in PNH patients.
Sexually dimorphic characters of the ultimate legs in lithobiid centipedes (Myriapoda, Chilopoda, and Lithobiomorpha): Morphology and implications for reproductive behavior
Authors: Roithmair Zita , Edgecombe Gregory D. , Wanninger Andreas , Akkari Nesrine 
Published: December 2022

AbstractMany species of lithobiomorph centipedes present a pronounced sexual dimorphism reflected in remarkable structural modifications on the ultimate legs of males. Most records of these male secondary sexual characters addressed taxonomy, helping to identify and characterize species or diagnose genera, but information on their diversity, detailed morphology and possible function(s) is scarce. In this study, nine species of the two lithobiid genera Lithobius Leach, 1814 and Eupolybothrus Verhoeff, 1907 were investigated, using light and scanning electron microscopy to document the detailed morphology of secondary sexual characters of male ultimate legs. Secondary sexual characters affecting the cuticle of the ultimate legs are described in detail and found to often be associated with sensilla, interpreted here as chemo‐ and mechanoreceptors, and with clusters of pores, a hitherto undescribed pore‐distribution for this group. The tibial nodule of the species Lithobius nodulipes Latzel, 1880, was additionally examined with histological semi‐thin sections. These results revealed that the clustered pores are connected to glandular tissue, and are, based on their morphology, interpreted as openings of flexo‐canal epidermal glands. The presence of various sensory and glandular structures associated with sexual dimorphism indicates a likely role during courtship and mating. The closely related species examined in this research show comparable dimorphic structures, which are otherwise species‐specific. Morphological observations on secondary sexual structures inform on reproductive biology in groups like lithobiomorphs for which there are limited behavioral data.
Total Thyroidectomy and Subsequent Weight Gain in Pediatric Populations
Authors: Edwards Evan R. , Hazkani Inbal , Stein Eli , Josefson Jami L. , Samis Jill H. , Miller Jennifer L. , Rastatter Jeffrey 
Published: December 2022

ObjectiveTo evaluate weight gain in children post‐thyroidectomy and identify predictors.MethodsCharts from patients at a tertiary health care facility who underwent total thyroidectomy from 2014 to 2020 were reviewed for Body Mass Index z‐scores (BMIz) at the time of thyroidectomy and at 1 and 2‐year post‐operation intervals. Patient demographic information, comorbidities, pre‐ and postoperative thyroid stimulating hormone, and postoperative free T4 levels were also extracted. Patients with other known endocrine abnormalities, chronic kidney disease, or without sufficient follow‐up were excluded.ResultsA total of 56 patients (ages 3–17 years old) met the inclusion criteria (n = 17 Graves' disease; n = 39 presumed cancer). Over the first year, average BMIz significantly increased in patients with Graves' disease (∆BMIz = 0.45 ± 0.77, p = 0.03), Hispanic ethnicity (∆BMIz = 0.43 ± 0.68, p = 0.004), Medicaid/no insurance coverage (∆BMIz = 0.33 ± 0.74, p = 0.038), age <13 years at thyroidectomy (∆BMIz = 0.35 ± 0.68, p = 0.016), and persistent postoperative hypothyroidism (∆BMIz = 0.41 ± 0.41, p = 0.012). These changes remained significant after the second year. Age at thyroidectomy correlated negatively with ∆BMIz only after the first year (r = −0.40, p = 0.002). Regression analysis, controlling for Graves' status, persistent postoperative hypothyroidism, and insurance coverage, identified age at thyroidectomy as a significant predictor of ∆BMIz after the first year (b = −0.06, p = 0.004) and Hispanic ethnicity as a significant predictor after the second year (b = 0.60, p = 0.003).ConclusionA small increase in BMIz post‐thyroidectomy was observed across several patient subgroups. Younger age at thyroidectomy and Hispanic ethnicity were associated with increased BMIz in the first 2 years post‐thyroidectomy.Level of EvidenceLevel 4 ‐ Historically controlled cohort Laryngoscope, 133:1518–1523, 2023
Effect of drying on kinetics, physiochemical, and antioxidant properties of black gram nuggets
Authors: Nahar Najmun , Raychaudhuri Utpal , Adhikari (Nee Pramanik) Sunita 
Published: December 2022

AbstractBlack gram (Vigna mungo) nuggets are locally known as “bori” and are an indigenous food and are consumed widely in the Indian subcontinent. The objective of the work was to compare the mode of selected drying techniques (hot‐air, freeze, and microwave drying) on drying kinetics followed by the development of suitable mathematical modeling for the process. Additionally, the antioxidant, color, and textural properties of the dried nuggets were evaluated for their acceptability and associated health benefits. Based on regression parameters, it was found that the Page model fitted well (R2 = 0.99) with the experimental data when compared with other models. The effective moisture diffusivity exhibited an inverse relation to drying time. Among tested drying techniques, it was found that TB in microwave drying at 450 W had the highest amount of phenolic content (5.27 mg/g), flavonoid content (1.72 mg/100 g), ferric reducing antioxidant power assay (45.71 μmol/g), 61.42 μmol/g of ABTS assay, and 67.81 μmol/g of DPPH assay values. The freeze‐drying products were better for physicochemical parameters than other drying process products. The presence of phytochemicals was responsible for the high bioactivity of microwave‐dried nuggets.
PLIC11 drives lung cancer progression through regulating the YY1/PIWIL4 axis
Authors: Zhu Yuyao , Chen Bing , Pan Hongyu , Sun Lei , Yu Tao 
Published: December 2022

AbstractLung cancer is the leading cause of cancer related deaths worldwide. Nonsmall cell lung cancers (NSCLC), the most common histological type of lung cancer, are known to be less well characterized. Long noncoding RNAs are a new class of cancer regulators. Here, we aimed to investigate the effect of lncRNA PLIC11 in NSCLC progression. In our study, we found that PLIC11 was upregulated in lung cancer, particularly in metastatic lung cancer tissues. Overexpression of PLIC11 enhanced cell proliferation, migration, and metastasis in vitro and in vivo. Mechanically, PLIC11 could interact with YY1 and promote PIWIL4 expression by transcription activation. Therefore, PLIC11 upregulation is a potential indicator of aggressive lung cancer, Silencing of PLIC11 has great potential therapeutic strategy in NSCLC.