bluuebunny/crossref_metadata_2025_split huggingface dataset:
Experimental Investigation of DFIG-based Wind Energy Conversion System Using Fuzzy Logic Control
Authors: Hallouz Mohamed , Kabeche Nadir , Moulahoum Samir , Kechidi Ziane 
Published: May 2023

In this paper, an experimental study of a Wind Energy Conversion System (WECS) is performed. A test bench with a power of 1.5 kW is setup. The system consists of a Doubly-Fed Induction Generator (DFIG) and a wind emulator based on a DC motor associated with a Maximum Power Point Tracking (MPPT) control. The proposed emulator is driven by a four quadrants DC/DC converter to produce a real wind turbine behavior. The aim of this work is to improve the DIFG performances by using the fuzzy logic-based intelligent controller. This control technic is designed to monitor the stator reactive and active powers. This can be achieved by the DFIG rotor side converter (RSC) using the field-oriented control. The experimental setup uses a dSPACE DS1104 device, MATLAB/Simulink software and a ControlDesk interface. The paper shows that, the desired amount of active and reactive powers has been independently controlled and the implementation is successfully verified the effectiveness of the proposed control scheme achieved using the FLC strategy.
Raman imaging for the analysis of silicone microplastics and nanoplastics released from a kitchen sealant
Authors: Fang Cheng , Luo Yunlong , Naidu Ravi 
Published: May 2023

Plastic products are used ubiquitously and can potentially release microplastics and nanoplastics into the environment, for example, products such as the silicone sealant used in kitchens. It is important to develop an effective method to monitor these emerging contaminants, as reported herein. By using advanced Raman imaging to characterize microplastics and nanoplastics from hundreds of spectra in a scanning spectrum matrix and not from a single spectrum or peak, the signal-to-noise ratio can be significantly increased, from a statistical point of view. The diffraction of the laser spot usually constrains the imaging resolution (such as at ∼300 nm), which is also pushed to the limit in this report by shrinking the scanning pixel size down to ∼50 nm to capture and image small nanoplastics effectively. To this end, image reconstruction is developed to successfully pick up the meaningful Raman signal and intentionally avoid the noise. The results indicate that the silicone sealant in a kitchen can release a significant amount of microplastics and nanoplastics. Overall, advanced Raman imaging can be employed to characterize the microplastics and even nanoplastics that are smaller than the diffraction limit of the laser via Raman imaging and image reconstruction toward deconvolution.
Improving osteoinduction and osteogenesis of Ti6Al4V alloy porous scaffold by regulating the pore structure
Authors: Wang Chao , Wu Jie , Liu Leyi , Xu Duoling , Liu Yuanbo , Li Shujun , Hou Wentao , Wang Jian , Chen Xun , Sheng Liyuan , Lin Huancai , Yu Dongsheng 
Published: May 2023

Titanium alloy scaffolds with a porous structure have attracted much attention in bone defect repair. However, which pore structure is more beneficial to bone defect repair is controversial. In the present research, the Ti6Al4V alloy porous scaffolds with gradient pore sizes were designed and fabricated. The microstructure characterization, tests of mechanical properties, and in vitro and in vivo experiments have been performed to systematically evaluate the effect of pore size on osteoinduction and osteogenesis. The results revealed that the contact angle with water, compressive strength, and elastic modulus of the Ti6Al4V alloy porous scaffolds decreased gradually with the increase of pore size. However, there were obvious drops when the pore size of the porous scaffold was around 600 μm. As the pore size increased, the proliferation and integrin β1 of RAW 264.7 macrophages seeded on Ti6Al4V alloy porous scaffolds increased at first, reaching a maximum value at a pore size of around 600 μm, and then decreased subsequently. The proliferation, integrin β1, and osteogenic gene-related expressions of Bone marrow mesenchymal stem cells (BMSCs) seeded on Ti6Al4V alloy porous scaffolds with different pore sizes all exhibited similar variations which rose with increased pore size firstly, obtaining the maximum value at pore size about 600 μm, and then declined. The in vivo experiments confirmed the in vitro results, and the Ti6Al4V alloy porous scaffold with a pore size of 600 μm possessed the better capability to induce new bone formation. Therefore, for the design of Ti6Al4V alloy with a regular porous scaffold, the surface morphology, porosity, strength, and elastic modulus should be considered systematically, which would determine the capability of osteoinduction and osteogenesis.
Building a nomogram plot based on the nanopore targeted sequencing for predicting urinary tract pathogens and differentiating from colonizing bacteria
Authors: Jiang Shengming , Wei Yangyan , Ke Hu , Song Chao , Liao Wenbiao , Meng Lingchao , Sun Chang , Zhou Jiawei , Wang Chuan , Su Xiaozhe , Dong Caitao , Xiong Yunhe , Yang Sixing 
Published: May 2023

BackgroundThe identification of uropathogens (UPBs) and urinary tract colonizing bacteria (UCB) conduces to guide the antimicrobial therapy to reduce resistant bacterial strains and study urinary microbiota. This study established a nomogram based on the nanopore-targeted sequencing (NTS) and other infectious risk factors to distinguish UPB from UCB.MethodsBasic information, medical history, and multiple urine test results were continuously collected and analyzed by least absolute shrinkage and selection operator (LASSO) regression, and multivariate logistic regression was used to determine the independent predictors and construct nomogram. Receiver operating characteristics, area under the curve, decision curve analysis, and calibration curves were used to evaluate the performance of the nomogram.ResultsIn this study, the UPB detected by NTS accounted for 74.1% (401/541) of all urinary tract microorganisms. The distribution of ln(reads) between UPB and UCB groups showed significant difference (OR = 1.39; 95% CI, 1.246–1.551, p &lt; 0.001); the reads number in NTS reports could be used for the preliminary determination of UPB (AUC=0.668) with corresponding cutoff values being 7.042. Regression analysis was performed to determine independent predictors and construct a nomogram, with variables ranked by importance as ln(reads) and the number of microbial species in the urinary tract of NTS, urine culture, age, urological neoplasms, nitrite, and glycosuria. The calibration curve showed an agreement between the predicted and observed probabilities of the nomogram. The decision curve analysis represented that the nomogram would benefit clinical interventions. The performance of nomogram with ln(reads) (AUC = 0.767; 95% CI, 0.726–0.807) was significantly better (Z = 2.304, p-value = 0.021) than that without ln(reads) (AUC = 0.727; 95% CI, 0.681–0.772). The rate of UPB identification of nomogram was significantly higher than that of ln(reads) only (χ2 = 7.36, p-value = 0.009).ConclusionsNTS is conducive to distinguish uropathogens from colonizing bacteria, and the nomogram based on NTS and multiple independent predictors has better prediction performance of uropathogens.
Estrogen receptor subtype mediated anti-inflammation and vasorelaxation via genomic and nongenomic actions in septic mice
Authors: Zhang Luyun , Wan Hanxing , Zhang Mengting , Lu Wei , Xu Feng , Dong Hui 
Published: May 2023

AimSepsis is a life-threatening disease with high mortality worldwide. Septic females have lower severity and mortality than the males, suggesting estrogen exerts a protective action, but nothing is known about the role of vascular endothelial estrogen receptor subtypes in this process. In the present study, we aimed to study the estrogen receptors on mesenteric arterioles in normal and sepsis mice and to elucidate the underlying mechanisms.MethodsSepsis was induced in mice by intraperitoneal injection of LPS. The changes in the expression and release of the serum and cell supernatant proinflammatory cytokines, including TNF-α, IL-1β and IL-6, were measured by qPCR and ELISA, and the functions of multiple organs were analyzed. The functional activities of mouse mesenteric arterioles were determined by a Mulvany-style wire myograph. The expression of phospholipase C (PLC) and inositol 1,4,5-trisphosphate receptor (IP3R) in endothelial cells were examined by Western blot and their functions were characterized by cell Ca2+ imaging.ResultsSeptic female mice had higher survival rate than the male mice, and pretreatment with E2 for 5 days significantly improved the survival rate and inhibited proinflammatory cytokines in septic male mice. E2 ameliorated pulmonary, intestinal, hepatic and renal multiple organ injuries in septic male mice; and ER subtypes inhibited proinflammatory cytokines in endothelial cells via PLC/IP3R/Ca2+ pathway. E2/ER subtypes immediately induced endothelial-derived hyperpolarization (EDH)-mediated vasorelaxation via PLC/IP3R/Ca2+ pathway, which was more impaired in septic male mice. E2/ER subtypes could rescue the impaired acetylcholine (ACh)-induced EDH-mediated vasorelaxation in septic male mice.ConclusionsE2 through ER subtypes mediates anti-inflammation and vasorelaxation via genomic and nongenomic actions in sepsis. Mechanistically, activation of endothelial ER subtypes reduces proinflammatory cytokines and induces EDH-mediated vasorelaxation via PLC/IP3R/Ca2+ pathway, leading to amelioration of sepsis-induced organ injury and survival rate.
Alveolar ridge preservation in post-extraction sockets using concentrated growth factors: a split-mouth, randomized, controlled clinical trial
Authors: Elayah Sadam Ahmed , Younis Hamza , Cui Hao , Liang Xiang , Sakran Karim Ahmed , Alkadasi Baleegh , Al-Moraissi Essam Ahmed , Albadani Mohammed , Al-Okad Wafa , Tu Junbo , Na Sijia 
Published: May 2023

AimThe aim of this clinical trial was to assess the impact of autologous concentrated growth factor (CGF) as a socket-filling material and its ridge preservation properties following the lower third molar extraction.Materials and methodsA total of 60 sides of 30 participants who had completely symmetrical bilateral impacted lower third molars were enrolled. The primary outcome variables of the study were bone height and width, bone density, and socket surface area in the coronal section. Cone beam computed tomography images were obtained immediately after surgery and three months after surgery as a temporal measure. Follow-up data were compared to the baseline using paired and unpaired t-tests.ResultsCGF sites had higher values in height and width when compared to control sites (Buccal wall 32.9 ± 3.5 vs 29.4 ± 4.3 mm, Lingual wall 25.4 ± 3.5 vs 23.1 ± 4 mm, and Alveolar bone width 21.07 ± 1.55vs19.53 ± 1.90 mm, respectively). Bone density showed significantly higher values in CGF sites than in control sites (Coronal half 200 ± 127.3 vs -84.1 ± 121.3 and Apical half 406.5 ± 103 vs 64.2 ± 158.6, respectively). There was a significant difference between both sites in the reduction of the periodontal pockets.ConclusionCGF application following surgical extraction provides an easy, low-cost, and efficient option for alveolar ridge preservation. Thus, the use of CGF by dentists during dental extractions may be encouraged, particularly when alveolar ridge preservation is required.Clinical trial registrationTCTR identification, TCTR20221028003.
Efficacy of ICI-based treatment in advanced NSCLC patients with PD-L1≥50% who developed EGFR-TKI resistance
Authors: Li Yujing , Jiang Haohua , Qian Fangfei , Chen Ya , Zhou Wensheng , Zhang Yanwei , Lu Jun , Lou Yuqing , Han Baohui , Zhang Wei 
Published: May 2023

IntroductionPlatinum-based chemotherapy is still the standard of care for Epidermal growth factor receptor (EGFR) mutated non-small cell lung cancer (NSCLC) patients after developing EGFR-TKI resistance. However, no study focusing on the role of immuno checkpoint inhibitor (ICI) based treatments for EGFR mutated NSCLC patients who carried programmed death ligand 1 (PD-L1) tumor proportion score (TPS) greater than 50% progressed after EGFR-TKI therapy. In this study, we retrospectively investigated the outcomes of ICI-based treatments for EGFR mutated NSCLC patients carried PD-L1 TPS≥50% after developing EGFR-TKI resistance and to explore the population that may benefited from ICI-based treatment.MethodsWe retrospectively collected data of advanced NSCLC patients with EGFR mutations and PD-L1 TPS≥50% who have failed prior EGFR-TKI therapies without T790M mutation at Shanghai Chest Hospital between January 2018 and June 2021. Progression-free survival (PFS) and overall survival (OS) were utilized to evaluate the outcomes of this study.ResultsA total of 146 patients were included. Up to June 20th, 2022, median follow-up was 36.7 months (IQR, 12.5-44.2 months). Among the population, 66 patients (45.2%) received chemotherapy, the remaning (54.8%) received ICI-based treatment, including 56 patients(70.0%) received ICI combined with chemotherapy (IC) and 24 patients (30.0%) received ICI monotherapy (IM). In IC group,31 patients received ICI combined with chemotherapy,19 patients received ICI combined with antiangiogenic therapy and remaing received ICI combined with chemotherapy and antiangiogenic therapy. Survival analysis shown that patients who received ICI-based treatment had better progress-free survival (PFS) and overall survival (OS) compared with those treated with other therapy (median PFS, 10.0 vs. 4.0 months, P&lt;0.001; median OS, 39.5 vs. 24.2 months, P&lt;0.001). What’s more, patients who treated with IC treatment had a superior survival time than those received IM treatment (median PFS, 10.3 vs. 7.0 months, P&lt;0.001; median OS, 41.6 vs. 32.4 months, P&lt;0.001). Subgroup analysis found that the PFS and OS benefit of IC was evident in all subgroups.ConclusionsFor advanced NSCLC patients with EGFR mutations and PD-L1 TPS≥50% who have failed prior EGFR-TKI therapies without T790M mutation, ICI-based treatment could provide a more favorable survival than classical chemotherapy. What’ s more, compared with ICI monotherapy, ICI combined with chemotherapy seems to be the preferred treatment.
Identification and immunological characterization of cuproptosis-related molecular clusters in idiopathic pulmonary fibrosis disease
Authors: Shi Xuefeng , Pan Zhilei , Cai Weixiu , Zhang Yuhao , Duo Jie , Liu Ruitian , Cai Ting 
Published: May 2023

BackgroundIdiopathic pulmonary fibrosis (IPF) has attracted considerable attention worldwide and is challenging to diagnose. Cuproptosis is a new form of cell death that seems to be associated with various diseases. However, whether cuproptosis-related genes (CRGs) play a role in regulating IPF disease is unknown. This study aims to analyze the effect of CRGs on the progression of IPF and identify possible biomarkers.MethodsBased on the GSE38958 dataset, we systematically evaluated the differentially expressed CRGs and immune characteristics of IPF disease. We then explored the cuproptosis-related molecular clusters, the related immune cell infiltration, and the biological characteristics analysis. Subsequently, a weighted gene co-expression network analysis (WGCNA) was performed to identify cluster-specific differentially expressed genes. Lastly, the eXtreme Gradient Boosting (XGB) machine-learning model was chosen for the analysis of prediction and external datasets validated the predictive efficiency.ResultsNine differentially expressed CRGs were identified between healthy and IPF patients. IPF patients showed higher monocytes and monophages M0 infiltration and lower naive B cells and memory resting T CD4 cells infiltration than healthy individuals. A positive relationship was found between activated dendritic cells and CRGs of LIPT1, LIAS, GLS, and DBT. We also identified cuproptosis subtypes in IPF patients. Go and KEGG pathways analysis demonstrated that cluster-specific differentially expressed genes in Cluster 2 were closely related to monocyte aggregation, ubiquitin ligase complex, and ubiquitin-mediated proteolysis, among others. We also constructed an XGB machine model to diagnose IPF, presenting the best performance with a relatively lower residual and higher area under the curve (AUC= 0.700) and validated by external validation datasets (GSE33566, AUC = 0.700). The analysis of the nomogram model demonstrated that XKR6, MLLT3, CD40LG, and HK3 might be used to diagnose IPF disease. Further analysis revealed that CD40LG was significantly associated with IPF.ConclusionOur study systematically illustrated the complicated relationship between cuproptosis and IPF disease, and constructed an effective model for the diagnosis of IPF disease patients.
Selection on female reproductive schedules in the marula fly, Ceratitis cosyra (Diptera: Tephritidae) affects dietary optima for female reproductive traits but not lifespan
Authors: Malod Kevin , Archer C. Ruth , Hunt John , Nicolson Susan W. , Weldon Christopher W. 
Published: May 2023

IntroductionA changing environment can select on life-history traits and trade-offs in a myriad of ways. For example, global warming may shift phenology and thus the availability of host-plants. This may alter selection on survival and fertility schedules in herbivorous insects. If selection on life-histories changes, this may in turn select for altered nutrient intake, because the blend of nutrients organisms consume helps determine the expression of life-history traits. However, we lack empirical work testing whether shifts in the timing of oviposition alter nutrient intake and life-history strategies.MethodsWe tested in the marula fruit fly, Ceratitis cosyra, how upward-selection on the age of female oviposition, in comparison with laboratory adapted control flies, affects the sex-specific relationship between protein and carbohydrate intake and life-history traits including lifespan, female lifetime egg production and daily egg production. We then determined the macronutrient ratio consumed when flies from each selection line and sex were allowed to self-regulate their intake.ResultsLifespan, lifetime egg production and daily egg production were optimised at similar protein to carbohydrate (P:C) ratios in flies from both selection lines. Likewise, females and males of both lines actively defended similar nutrient intake ratios (control =1:3.6 P:C; upward-selected = 1:3.2 P:C).DiscussionOur results are comparable to those in non-selected C. cosyra, where the optima for each trait and the self-selected protein to carbohydrate ratio observed were nearly identical. The nutrient blend that needs to be ingested for optimal expression of a given trait appeared to be well conserved across laboratory adapted and experimentally selected populations. These results suggest that in C. cosyra, nutritional requirements do not respond to a temporal change in oviposition substrate availability.
An exome-wide study of renal operational tolerance
Authors: Massart Annick , Danger Richard , Olsen Catharina , Emond Mary J. , Viklicky Ondrej , Jacquemin Valérie , Soblet Julie , Duerinckx Sarah , Croes Didier , Perazzolo Camille , Hruba Petra , Daneels Dorien , Caljon Ben , Sever Mehmet Sukru , Pascual Julio , Miglinas Marius ,   the Renal Tolerance Investigators, Pirson Isabelle , Ghisdal Lidia , Smits Guillaume , Giral Magali , Abramowicz Daniel , Abramowicz Marc , Brouard Sophie 
Published: May 2023

BackgroundRenal operational tolerance is a rare and beneficial state of prolonged renal allograft function in the absence of immunosuppression. The underlying mechanisms are unknown. We hypothesized that tolerance might be driven by inherited protein coding genetic variants with large effect, at least in some patients.MethodsWe set up a European survey of over 218,000 renal transplant recipients and collected DNAs from 40 transplant recipients who maintained good allograft function without immunosuppression for at least 1 year. We performed an exome-wide association study comparing the distribution of moderate to high impact variants in 36 tolerant patients, selected for genetic homogeneity using principal component analysis, and 192 controls, using an optimal sequence-kernel association test adjusted for small samples.ResultsWe identified rare variants of HOMER2 (3/36, FDR 0.0387), IQCH (5/36, FDR 0.0362), and LCN2 (3/36, FDR 0.102) in 10 tolerant patients vs. 0 controls. One patient carried a variant in both HOMER2 and LCN2. Furthermore, the three genes showed an identical variant in two patients each. The three genes are expressed at the primary cilium, a key structure in immune responses.ConclusionRare protein coding variants are associated with operational tolerance in a sizable portion of patients. Our findings have important implications for a better understanding of immune tolerance in transplantation and other fields of medicine.ClinicalTrials.gov, identifier: NCT05124444.
Genomic characteristics of Mycobacterium tuberculosis isolates of cutaneous tuberculosis
Authors: Mei You-Ming , Zhang Wen-Yue , Sun Ji-Ya , Jiang Hai-Qin , Shi Ying , Xiong Jing-Shu , Wang Le , Chen Yan-Qing , Long Si-Yu , Pan Chun , Luo Tao , Wang Hong-Sheng 
Published: May 2023

ObjectivesCutaneous tuberculosis with various manifestations can be divided into several clinical types according to the host's immune status and infective route. However, the etiological factors of this disease remain unclear. The objective of this study is to investigate the pathogens associated with the occurrence and different types of cutaneous tuberculosis.Methods58 Mycobacterium tuberculosis strains isolated from cutaneous tuberculosis over the last 20 years were sequenced and analyzed for genomic characteristics including lineage distribution, drug-resistance mutations, and mutations potentially associated with different sites of infection.ResultsThe M. tuberculosis strains from four major types of cutaneous tuberculosis and pulmonary tuberculosis shared similar genotypes and genomic composition. The strains isolated from cutaneous tuberculosis had a lower rate of drug resistance. Phylogenic analysis showed cutaneous tuberculosis and pulmonary tuberculosis isolates scattered on the three. Several SNPs in metabolism related genes exhibited a strong correlation with different infection sites.ConclusionsThe different infection sites of TB may barely be affected by large genomic changes in M. tuberculosis isolates, but the significant difference in SNPs of drug resistance gene and metabolism-related genes still deserves more attention.
Learning implicit sentiments in Alzheimer's disease recognition with contextual attention features
Authors: Liu Ning , Yuan Zhenming , Chen Yan , Liu Chuan , Wang Lingxing 
Published: May 2023

BackgroundAlzheimer's disease (AD) is difficult to diagnose on the basis of language because of the implicit emotion of transcripts, which is defined as a supervised fuzzy implicit emotion classification at the document level. Recent neural network-based approaches have not paid attention to the implicit sentiments entailed in AD transcripts.MethodA two-level attention mechanism is proposed to detect deep semantic information toward words and sentences, which enables it to attend to more words and fewer sentences differentially when constructing document representation. Specifically, a document vector was built by progressively aggregating important words into sentence vectors and important sentences into document vectors.ResultsExperimental results showed that our method achieved the best accuracy of 91.6% on annotated public Pitt corpora, which validates its effectiveness in learning implicit sentiment representation for our model.ConclusionThe proposed model can qualitatively select informative words and sentences using attention layers, and this method also provides good inspiration for AD diagnosis based on implicit sentiment transcripts.