bluuebunny/crossref_metadata_2025_split huggingface dataset:
ATF3 Modulates the Proliferation, Migration, and Apoptosis of
Synovial Fibroblasts after Arthroscopy by Promoting RGS1
Transcription
Authors: He Xiao , Deng Lili , Zou Kang , Tian Yun , Tang Xianzhe 
Published: April 2023

Background:
Osteoarthritis (OA) is a degenerative joint disease involving
both cartilage and synovium. Activating transcription factor 3 (ATF3) and regulator of G
protein signaling 1 (RGS1) have been reported to be up-regulated in OA. However, little
is known regarding the relationship between these two genes and the mechanism of this
relationship in OA development. Therefore, the present study explores the mechanism of
ATF3-mediated RGS1 in the proliferation, migration, and apoptosis of synovial
fibroblasts.

Methods:
After the OA cell model was constructed with TGF-β1 induction, human
fibroblast-like synoviocytes (HFLSs) were transfected with ATF3 shRNA or RGS1
shRNA alone or co-transfected with ATF3 shRNA and pcDNA3.1-RGS1. Then,
proliferation, migration, apoptosis, and the expression of ATF3, RGS1, α-SMA, BCL-2,
caspase3, and cleaved-caspase3 were measured. Meanwhile, the potential relationship
between ATF3 and RGS1 was predicted and validated.

Results and Discussion:
Analysis of the GSE185059 dataset suggested that RGS1
was up-regulated in OA synovial fluid exosomes. Moreover, ATF3 and RGS1 were both
highly expressed in TGF-β1-induced HFLSs. Transfection of ATF3 shRNA or RGS1
shRNA significantly reduced proliferation and migration and promoted apoptosis of TGF-
β1-induced HFLSs. Mechanistically, ATF3 bound to the RGS1 promoter and elevated
RGS1 expression. Silencing ATF3 repressed proliferation and migration and enhanced
apoptosis of TGF-β1-induced HFLSs by down-regulating RGS1.

Conclusion:
ATF3 binds to the RGS1 promoter and enhances RGS1 expression to
accelerate cell proliferation and block cell apoptosis in TGF-β1-induced synovial
fibroblasts.
Synthetic and Mechanistic Investigations in cis-3-(Substituted acetoxy)
azetidin-2-ones from cis-3-Hydroxyazetidin-2-ones: Potential
Synthons for Pharmacophoric Hybridized Molecules
Authors: Bhalla Aman , Modi Garima , Kaur Jaswinder , Kumar Pankaj , Bari Shamsher S. 
Published: April 2023

Abstract:
The present work describes the synthesis of cis-3-(substituted acetoxy)azetidin-2-ones from
cis-3-hydroxyazetidin-2-ones. Two different routes have been investigated for the substitution at the
C-3 position of the azetidin-2-ones. Method A involves the use of acetyl chloride XCOCl in the presence
of pyridine and method B consists of using appropriate acid XCOOH in a catalytic amount of
DMAP which was found to be the best to furnish the target azetidin-2-one. All the newly synthesized
compounds were characterized on the basis of various spectroscopic techniques (FT-IR, 1H NMR, 13C
NMR, and elemental analysis). Two different routes have been investigated for the substitution at the
C-3 position of the azetidin-2-ones.
Knockdown of VASH2 Inhibits the Stemness and EMT Process by Regulating
ZEB2 in Colorectal Cancer
Authors: Shayimu Paerhati , Yin Cheng , Zeng Xiangyue , Jiapaer Rexida 
Published: April 2023

Introduction:
VASH2 is associated with the malignant progression of a variety of tumors, but
the role and mechanism of VASH2 in colorectal cancer are still unclear.

Methods:
We analyzed the expression of VASH2 in colorectal cancer from the TCGA database and also
analyzed the relationship between VASH2 expression and survival of colorectal cancer patients in the
PrognoScan database. We verified the role of VASH2 in colorectal cancer through transfecting si-VASH2
into colorectal cancer cells and detecting cell viability by CCK8, cell migration by wound healing assay,
and cell invasion by Transwell assay. ZEB2, Vimentin, and E- cadherin protein expression were examined
by Western-Blot assay. Cell sphere-forming ability was determined by sphere formation assay, and
we further confirmed the mechanism of VASH2 in colorectal cancer progression by rescue assays.

Results:
Colorectal cancer has a high expression of VASH2, and its expression is associated with a poorer
patient survival rate. The vitality, migration, invasion, EMT, and tumor stemness of colorectal cancer
cells were all decreased by VASH2 knockdown. These alternations were attenuated by ZEB2 overexpression.

Conclusion:
Our experiments confirmed that VASH2 affects colorectal cancer cell proliferation, migration,
invasion, EMT, and seed bovine stemness by regulating ZEB2 expression.
The Effectiveness of UV-C Light for Inactivating Listeria monocytogenes Bacteria and its Impact on Apple Juice
Authors: Tirono Mokhamad 
Published: April 2023

Background:
Apple juice is useful for preventing cardiovascular disease, degenerative disease, and cancer. The supply of apple juice is often contaminated with microbes due to raw materials and different processing methods. This study aimed to determine the effectiveness of inactivating L. monocytogenes bacteria using UV-C light by observing its impact on vitamin C content, pH, and color of apple juice.

Methods:
The research sample was Manalagi apple juice contaminated with L. monocytogenes bacteria. The treatment used UV-C with a wavelength of 185-280 nm and the optimum intensity at 256 nm. The light intensity used for the treatment was 0-125 mW/cm2, with treatment times of 30 minutes and 60 minutes.

Results:
The results showed that the inactivation of bacteria with a light intensity of 125 mW/cm2 for 30 minutes reduced the number of bacteria by 0.49 ± 0.02 log10, while the inactivation for 60 minutes was able to reduce the number of bacteria by 1.01 ± 0.02 log10. Treatment using UV-C reduced the content of vitamin C, raised the pH, and changed the color of apple juice.

Conclusion:
Treatment using UV-C with higher intensity was effective in reducing the number of large bacteria but had the potential to decrease the vitamin C content and change the color of apple juice to brown. Treatment using UV-C with an intensity of 100 and 125 mW/cm2 affected the smell of apple juice.
Obstacle Avoidance Path Planning of 7-DOF Redundant Manipulator
Based on Improved Ant Colony Optimization
Authors: Wang Shuaixiu , Wang Wenjie , Cao Yuting , Luo Yang , Wang Xiaohua 
Published: April 2023

Background:
Obstacle avoidance path planning is an important parameter of robot manipulators.
Path planning can directly affect the working efficiency of the manipulator.

Objective:
This study aims to summarize the optimization design method from a large number of literature
and propose a new optimization design method to make the planned obstacle avoidance path of the
manipulator shorter and smoother.

Methods:
The forward and inverse kinematics of the redundant manipulator is solved. Secondly, the obstacle
and the robot manipulator envelope are simplified for collision detection. Then, the ant colony algorithm
is improved by adding an obstacle environment to the construction of the heuristic function and
dynamically adjusting the heuristic function factor to make the shortest path distance planned by the algorithm.
Finally, the worst ant colony is added to the pheromone update to avoid the algorithm falling
into the local optimal solution.

Results:
Through experiments and comparative studies, the optimized design process shows that the
path planned by the improved ant colony algorithm has obvious advantages of shorter path distance and
smoother path distance, which verifies the rationality of the improved algorithm.

Conclusion:
This method optimizes the convergence speed of the ant colony algorithm and avoids the
ant colony algorithm from falling into the local optimal solution, which is of great significance for improving
the obstacle avoidance path planning problem of a redundant manipulator with a degree of
freedom.
Identification of Competing Endogenous RNA Networks and Potential
Therapeutic Drugs for Gastroesophageal Reflux Disease
Authors: Su Xin , Zhang Danping , Zhu Hong 
Published: April 2023

Background:
The diagnosis and cure rate of gastroesophageal reflux disease (GERD) is low, and its pathogenesis remains unclear.

Aims:
In this study, we aimed to construct the competing endogenous RNA (ceRNA) of GERD by bioinformatics and explore the potential therapeutic drugs for GERD.

Methods:
The differentially expressed genes (DEGs) were got by limma package and visualized by ggplot2 and pheatmap package in R version 4.1.3 from the GSE63401 gene microarray data. The pro-tein-protein interaction (PPI) network was constructed by the STRING database. The hub genes and subnetwork modules were obtained by Cytoscape software. The microRNA and lncRNA were pre-dicted by the miRTarBase and miRNet databases to construct the ceRNA network. Potential drugs tar-geting DEGs were screened by the CMAP database.

Results:
In this study, a total of 571 GEDs were obtained, including 462 up-regulated genes and 109 down-regulated genes, which mainly participated in the leukocyte migration process and the cytokine-cytokine receptor interaction pathway. Seventeen miRNAs and three key lncRNAs involved in regulat-ing hub genes were screened through miRTarBase and miRNet databases. Finally, nine ceRNA net-works were obtained, including TTN-AS1/NEAT1/XIST - miR-139-5p - CXCR4 axis, TTN-AS1/NEAT1/XIST - miR-185-5p - IL1B axis and TTN-AS1/NEAT1/XIST - miR-495-3p - LYN axis. In addition, five potential drugs (Pimavanserin, BMS-182874, CL-218872, Losartan, and Laropiprant) for GERD were obtained through the CMAP database.

Conclusion:
We constructed nine GERD-related ceRNA networks and screened five potential thera-peutic drugs for GERD, providing a theoretical basis for further research on the pathogenesis of GERD.
Topic Modeling on Online News.Portal Using Latent Dirichlet Allocation (LDA)
Authors: Fahlevvi Mohammad Rezza , SN Azhari 
Published: April 2023

The amount of News displayed on online news portals. Often does not indicate the topic being discussed, but the News can be read and analyzed. You can find the main issues and trends in the News being discussed. It would be best if you had a quick and efficient way to find trending topics in the News. One of the methods that can be used to solve this problem is topic modeling. Theme modeling is necessary to allow users to easily and quickly understand modern themes' development. One of the algorithms in topic modeling is the Latent Dirichlet Allocation (LDA). This research stage begins with data collection, preprocessing, n-gram formation, dictionary representation, weighting, topic model validation, topic model formation, and topic modeling results. Based on the results of the topic evaluation, the. The best value of topic modeling using coherence was related to the number of passes. The number of topics produced 20 keys, five cases with a 0.53 coherence value. It can be said to be relatively stable based on the standard coherence value.
Secado de PET contrastando hornos de microondas y eléctrico
Authors: Zamorano romero Cosme , Palacios alquisira Joaquín 
Published: April 2023

El objetivo de este trabajo consistirá en revisar conceptos termodinámicos como la Primera Ley de la Termodinámica y calor, y cómo pueden aplicarse en el cálculo de la energía necesaria para el secado de un material humedo. Se desarrolla una comparación, entre la energía necesaria para disminuir la humedad de hojuela de PET (Polietilen Tereftalato) mediante el uso de un horno eléctrico y otro que usa microondas.Se presenta un trabajo práctico, útil para bachillerato y primer semestre de carreras de ingeniería química. Se tomaron los datos de experimentos realizados y corroborados en el laboratorio (Hernández, 2006), los cuales se realizaron con muestras de hojuela de PET obtenidas de botellas de bebidas carbonatadas, con las mismas propiedades fisicoquímicas. De los resultados observados se puede concluir que el uso de microondas en el secado de PET reduce el consumo de energía.
Karakteristik Kimia dan Sensori Minuman Instan Kombucha dari Kulit Buah Naga Merah (Hylocereus polyrhizus) Berdasarkan Konsentrasi Gula dan Lama Fermentasi
Authors: Achmad Naufal  , Harini Noor , Nuriza Putri Desiana 
Published: April 2023

Kombucha merupakan produk minuman dari hasil fermentasi antara substrat dengan gula oleh mikroorganisme yang terdiri dari bakteri dan jamur. Bahan yang dapat digunakan untuk membuat kombucha adalah kulit buah naga yang memiliki kandungan tinggi antioksidan. Metode foam mat drying menjadi solusi untuk mempertahankan kandungan gizi kombucha. Penelitian ini bertujuan untuk mengetahui interaksi antara konsentrasi gula dan lama fermentasi terhadap karakteristik kimia dan sensori kombucha instan. Penelitian ini menggunakan Rancangan Acak Kelompok (RAK) faktorial yang terdiri dari dua faktor, yaitu faktor (G) konsentrasi gula terdiri dari 3 taraf (5%, 10%, 15%) dan faktor (L) lama fermentasi yang terdiri dari 3 taraf (6 hari, 8 hari, 10 hari) dilakukan sebanyak 3 kali ulangan. Paramater yang diamati meliputi aktivitas antioksidan, total fenol, rendemen, kadar air, kadar abu, pH, kelarutan, vitamin C dan uji organoleptik. Data dianalisis dengan analisa ragam dilanjutkan dengan uji DMRT taraf 5%. Hasil penelitian menunjukkan terdapat interaksi antar perlakuan terhadap pH, organoleptik aroma, rasa, warna dan kesukaan dari kombucha instan. Pada hasil penelitian diperoleh aktivitas antioksidan pada perlakuan L2 sebesar 79,67%, kadar air pada perlakuan L3 sebesar 3,35%, kadar abu pada perlakuan G3 sebesar 0,67%, kelarutan pada perlakuan L3 sebesar 97,89%, total fenol pada perlakuan L2 sebesar 0,40 mg GAE/g, uji pH pada perlakuan G1L1 sebesar 4,37, rendemen pada perlakuan G3 sebesar 29,02%, vitamin C pada perlakuan L2 0,72%, aroma pada perlakuan G1L1 sebesar 4,52 (Netral), skor rasa pada perlakuan G2L1 sebesar 4,92 (Agak suka), skor warna pada perlakuan G1L1 sebesar 6,00 (Suka), skor kesukaan pada perlakuan G2L1 sebesar 4,72 (Agak suka)
Revision risk by using the direct superior approach (DSA) for total hip arthroplasty compared with postero-lateral approach: early nationwide results from the Dutch Arthroplasty Register (LROI)
Authors: Van Dooren Bart , Peters Rinne M , Ettema Harmen B , Schreurs B Willem , Van Steenbergen Liza N , Bolder Stefan B T , Zijlstra Wierd P 
Published: April 2023

Background and purpose: The direct superior approach (DSA) is a modification of the classic posterolateral approach (PLA) for total hip arthroplasty (THA), in which the iliotibial band and short external rotators are spared. The revision rate of the DSA has not been investigated previously using arthroplasty registry data. We examined the reasons and risk of revision of the DSA, compared with the direct anterior approach (DAA) and PLA.Patients and methods: In this population-based cohort study we included 175,543 primary THAs performed between 2014 and 2020 (PLA, n = 117,576; DAA, n = 56,626; DSA, n = 1,341). Competing risk survival analysis and multivariable Cox proportional hazard analyses, adjusted for potential confounders, were performed.Results: After 3 years, crude revision rates due to any reason were 2.1% (95% confidence interval [CI] 1.3–3.3) for DSA, and 2.9% (CI 2.8–3.0) for PLA. Crude dislocation revision rates were 0.3% (CI 0.1–0.8) for DSA, versus 1.0% (CI 0.9–1.0) for PLA. Dislocation revision rate for DSA did not differ from DAA (0.3% [CI 0.2–0.3]). Multivariable Cox regression analysis demonstrated no overall difference in revision rates for the DSA (HR 0.6 [CI 0.4–1.09) compared with the PLA. Lower risk of revision due to dislocation was found in patients operated on through the DSA (HR 0.3 [0.1–0.9]) compared with the PLA.Conclusion: Early nationwide results suggest that the DSA for total hip arthroplasty seems to show a tendency towards a lower risk of revision for dislocation but no overall reduced revision risk compared with the PLA.
Аномальные маточные кровотечения раннего репродуктивного периода и COVID-19.
Authors: Курмангалеева Алия , Сибирская Елена 
Published: April 2023

Аномальные маточные кровотечения являются одной из актуальных проблем здравоохранения, занимают лидирующее место среди показаний для госпитализации в стационар, и нет тенденции к снижению данного показателя. В структуре детской гинекологической патологии встречаются с частотой 25-30% случаев всех обратившихся девочек в возрасте от менархе до 17 лет включительно, у 33-69% заболевание склонно к рецидивам и хроническому течению.
Впервые в г. Москва в детском многопрофильном стационаре проводится работа, для определения частоты возникновения аномальных маточных кровотечений у девочек раннего репродуктивного периода (от наступления менархе до 17 лет включительно) с имеющейся или перенесенной новой коронавирусной инфекцией. Будет определена наиболее оптимальная тактика лечения и диагностики в зависимости от степени тяжести COVID-19, созданы алгоритмы ведения пациентов.
Risk Perception as Mediator in the Effect of Financial Literacy to Entrepreneurial Orientation among MSMEs
Authors: Carina Tiara , Mentari Ni Made Indah , Permana Putra I Dewa Made Arik , Sari Melani Gita , Purnama Dewi Ni Putu Tasiya 
Published: April 2023

This study aims to analyze the mediating role of risk perception on the effect of financial literacy to entrepreneurial orientation. From a total population of 32,026 MSMEs in Denpasar, 244 samples were taken through purposive sampling. Data were collected through the administration of Financial Literacy Instruments (rxx=0.639-0.891; r=0.974), Risk Perception Instruments (rxx=0.873-0.956; r=0.989), and Entrepreneurship Orientation Instruments (rxx=0.894-0.988; r=0.988). According to data analysis with Partial Least Square (PLS), it was found that financial literacy significantly affects entrepreneurial orientation; financial literacy significantly influences risk perception; risk perception significantly influences entrepreneurial orientation; and risk perception partially mediates the effect of financial literacy to entrepreneurial orientation in MSMEs in Denpasar. It was found that 49.8% of the variation in the entrepreneurial orientation variable is explained by financial literacy and risk perception variables, while the remaining 50.2% is contribution of other variables outside this research.
Keyword: risk perception, financial literacy, entrepreneurship orientation, MSME
Two Free Boundaries Problem for a Parabolic Equation
Authors: Расулов М.С. 
Published: April 2023

В данной работе рассматривается задача типа Стефана с двумя свободными границами для квазилинейного параболического уравнения в одномерном случае. Исследование нелинейных задач со свободными границами методом, основанным на построении априорных оценок. Поэтому сначала устанавливаются некоторые первоначальные априорные оценки для решения рассматриваемой задачи. Основной трудностью при построении теории для задач квазилинейных параболических уравнений второго порядка является получение априорной оценки модуля производной решение, а также в задачах со свободной границей требуются дополнительные рассуждения. Для этого задача сводится к задаче с фиксированной границей через замену переменных. Полученная задача имеет зависящие от времени и положения в пространстве коэффициенты с нелинейными слагаемыми. Далее построены априорных оценок типа Шаудера для решения уравнения с нелинейными слагаемыми и закрепленной границей. На основе полученных оценок доказана единственность решения задачи. Затем мы доказываем глобальное существование решения задачи с помощью теоремы Лерэ-Шаудера о неподвижной точке.
This paper considers a two-free-boundary Stefan-type problem for a quasi-linear parabolic equation in one dimension. Nonlinear problems with free boundaries are studied using a method based on constructing a priori estimates. Therefore, some initial a priori estimates for the solution to the problem under consideration are first established. The main difficulty in constructing a theory for second-order quasi-linear parabolic equations is obtaining an a priori estimate for the solution’s derivative module, and additional arguments are required in problems with a free boundary. To address this, the problem is reduced to a fixed-boundary problem through a change of variables. The resulting problem has time- and spacedependent coefficients with nonlinear terms. Next, Schauder-type a priori estimates are constructed for the equation with nonlinear terms and a fixed boundary. Based on these estimates, the uniqueness of the solution to the problem is proven. Then, the global existence of the solution to the problem is demonstrated using the Leray-Schauder fixed-point theorem.
The Problem for a Mixed Equation with Fractional Power of the Bessel Operator
Authors: Дзарахохов А.В. , Шишкина Э.Л. 
Published: April 2023

В последнее время особый интерес представляют уравнения с частными производными, содержащими дифференциальный оператор дробного порядка. Подобные уравнения и задачи для них находят применение в теории вязкой упругости, электрохимии, теории управления, моделировании эпидемий и пандемий и в других различных областях. Настоящая работа посвящена решению дифференциальных уравнений, содержащих оператор Бесселя дробной степени. В статье рассматривается прямое и обратное преобразование Мейера, модифицированное для удобства работы с оператором Бесселя дробной степени. Для рассматриваемого преобразования Мейера получена свертка. Используя преобразования Лапласа и Пуассона получены факторизации прямого и обратного преобразований Мейера. С использованием рассмотренного модифицированного преобразования Мейера находится решение обыкновенного дифференциального уравнения с оператором Бесселя дробной степени. Рассматривается нелокальная краевая задача для смешанного параболо-гиперболческого уравнения, содержащего дробной степени оператор Бесселя. Доказывается, что, при выполнении определенных условий гладкости входных функций задачи и выполнения условия сопряжения на линии раздела областей гиперболичности и параболичности, регулярное решение нелокальной краевой задачи для смешанного параболо-гиперболического уравнения с оператором Бесселя дробной степени существует и единственно.
Recently, of particular interest are partial differential equations containing a fractional order differential operator. Similar equations and problems for them find application in the theory of viscous elasticity, electrochemistry, control theory, modeling of epidemics and pandemics, and in various other areas. The present work is devoted to the solution of differential equations containing the Bessel operator of fractional degree. The article discusses the direct and inverse Meyer transforms, modified for the convenience of working with the Bessel operator of a fractional degree. For the considered Meyer transformation, a convolution is obtained. Using the Laplace and Poisson transformations, factorizations of the direct and inverse Meyer transformations are obtained. Using the considered modified Meyer transform, we find a solution to an ordinary differential equation with a Bessel operator of fractional degree. A nonlocal boundary value problem for a mixed parabolic-hyperbolic equation containing a fractional degree Bessel operator is considered. It is proved that, under certain conditions of smoothness of the input functions of the problem and the condition of conjugation on the dividing line of the regions of hyperbolicity and parabolicity, a regular solution of a nonlocal boundary value problem for a mixed parabolic-hyperbolic equation with a Bessel operator of fractional degree exists and is unique.