bluuebunny/crossref_metadata_2025_split huggingface dataset:
In-Game Uncertainties and Climate Change Challenges as Identified by Pre-Service Teachers
Authors: van Schaik Fiona 
Published: September 2023

Background This study explores what pre-service teachers identify as uncertainties within two climate board games and to what aspects of reality they relate these uncertainties, if at all. Should games be suitable resources for teaching topics related to climate change uncertainty, I expect pre-service teachers to (a) identify uncertainties within climate board games, and (b) relate these uncertainties to reality. Intervention To explore whether and how pre-service teachers identify and relate in-game uncertainties to reality, pre-service teachers played and discussed two climate board games ( Another Future and CATAN: Oil Springs Scenario). Methods A content analysis of pre-service teachers’ audio-recorded discussions about the games was conducted. First, a conventional content analysis indicated the uncertainties that they identified. Second, further analysis showed how the pre-service teachers related these uncertainties to reality. Results This study indicates that pre-service teachers identify various uncertainties from two different board games, including the occurrence/impact of catastrophes and the behaviour of players, who can ruin the game. In most cases, the pre-service teachers link these uncertainties to reality. Pre-service teachers discuss, for example, political dilemmas and interests, climate justice issues, challenges of and adaptation to possible catastrophes, and the significance of environmental activism. Discussion The research findings are discussed in relation to existing literature on uncertainty in climate change games. Teacher educators and teachers using games for education about climate change uncertainties are recommended to encourage dialogues about these uncertainties, use or take into account context, and consider games as tools for learning to live with climate change uncertainty.
ADHD Diagnosis in Children of Non-US-Born Parents: A Cross-Sectional Analysis
Authors: Pham Duy , Lin Allison , Rosenthal Hannah , Milanaik Ruth 
Published: September 2023

Objective(s): To assess the likelihood of ADHD diagnosis in children of non-US-born caregivers relative to children of US-born caregivers. Method: Cross-sectional analysis of a combined 2016 to 2019 National Survey of Children’s Health dataset ( n = 109,881) was performed to identify associations between caregiver’s birth outside the U.S. and child’s ADHD diagnosis. Logistic regression models adjusted for potential sociodemographic confounders. Results: Children with one or two non-US-born caregivers were less likely to be diagnosed with ADHD (aOR = 0.58, p < .001; aOR = 0.59, p < .001, respectively). Likelihood of a diagnosis increased as non-US-born caregivers spent more time in the US. After a diagnosis, children of two non-US-born caregivers were less likely to be treated with medication. Conclusion: The lower likelihood of ADHD diagnosis in children with non-US-born caregivers may reflect an increasing need for public health education to raise awareness about ADHD among this population and the development of culturally sensitive ADHD identification methodologies.
Efficacy and safety of four-year ofatumumab treatment in relapsing multiple sclerosis: The ALITHIOS open-label extension
Authors: Hauser Stephen L , Zielman Ronald , Das Gupta Ayan , Xi Jing , Stoneman Dee , Karlsson Goeril , Robertson Derrick , Cohen Jeffrey A , Kappos Ludwig 
Published: September 2023

Background: Ofatumumab has demonstrated superior efficacy and favorable safety for up to 2.5 years versus teriflunomide in relapsing multiple sclerosis (RMS). Objective: Further characterize efficacy and safety of ofatumumab in RMS. Methods: Efficacy set: patients randomized to ofatumumab/teriflunomide in ASCLEPIOS I/II (core). Safety set: patients who received ⩾ 1 dose of ofatumumab in ASCLEPIOS I/II, APLIOS, APOLITOS (all core), or ALITHIOS (umbrella open-label extension). Patients received continuous ofatumumab or were newly switched from teriflunomide. Data cut-off: 25 September 2021. Results: In the efficacy set ( n = 1882), the continuous ofatumumab group had a low annualized relapse rate (ARR 0.05 (95% confidence interval: 0.04–0.07)), low numbers of gadolinium-enhancing (Gd+) T1 lesions (0.01 lesions/scan) and fewer new/enlarging T2 lesions (annualized rate 0.08). Overall, 78.8% met three-parameter “no evidence of disease activity” criteria through 4 years. Switching from teriflunomide led to reduced ARR, risk of confirmed disability worsening (CDW), new/enlarging T2 lesions, Gd+ T1 lesions, and serum neurofilament light chain. In the continuous and newly switched ofatumumab groups, cumulative 3- and 6-month CDW rates remained low. In the safety set ( n = 1969), the most frequently reported adverse events were infections and infestations (58.35%). No new safety signals were identified. Conclusion: Ofatumumab has a favorable longer-term benefit–risk profile in RMS. Trial registry: ALITHIOS (NCT03650114): https://clinicaltrials.gov/ct2/show/NCT03650114
Role of Public Debt in Indian States in the Post-Reform Era
Authors: Roy Subaran , Paul Anusree 
Published: September 2023

The substantial amount of existing literature investigating the debt–growth association for high-income countries shows that debt has a negative impact on economic growth or the existence of an inverted U-shaped relationship between these two variables on some occasions. Given the lack of literature involving developing economies in this space, our study entirely focuses on a developing country like India. The study examines the empirical relationship between public debt (internal) and state gross domestic product (SGDP) for India in the post-reform era. The panel data extend from the year 2000 till 2020. Our random error component model findings indicate a positive and significant impact of debt in enhancing the Indian SGDPs for the above-mentioned period. Results are also confirmed by using an alternative estimation technique, namely, fixed effect. Next, we address the issue of endogeneity by employing generalized method of moments. Our results continue to remain robust. Finally, the inclusion of other control variables does not alter our findings. JEL Codes: H63, H54, O50, C51
Demographics and deprivation in obstetric brachial plexus palsy: a retrospective cohort study
Authors: Hardie Claire Madeline , Bourke Grainne , Salt Emily , Fort-Schaale Alice , Clark Stephen , Wiberg Mikael , Bains Robert 
Published: September 2023

The present study analyses the relationships between deprivation and obstetric brachial plexus palsy (OBPP). A retrospective observational study was conducted of infants with OBPP seen between 2008 and 2020 ( n = 321). The index of multiple deprivation (IMD) was used to assign an IMD rank to patients based on birth postcode and the relationship with OBPP was analysed, including deprivation, gestational diabetes, age at referral and at first assessment. Quintile-based analysis demonstrated over-representation of patients from more deprived neighbourhoods ( n = 109, 39%) living in the top 20% most deprived neighbourhoods. A total of 48 (15%) mothers had diabetes and 98 (31%) infants underwent surgical brachial plexus exploration (a marker of disease severity). Neither diabetes, age at referral nor age at first assessment were associated with IMD score. This suggests that neighbourhood deprivation is associated with OBPP, though the mechanisms are unclear. Further studies in this area may enable targeted health intervention for more deprived maternal and infant groups. Level of evidence: III
Tips and Tricks for Anatomic ACL Reconstruction With Soft-Tissue Quadriceps Tendon and Remnant Repair
Authors: Dadoo Sahil , Keeling Laura E. , Runer Armin , Musahl Volker 
Published: September 2023

Background: Quadriceps tendon (QT) autograft represents an excellent option for anterior cruciate ligament (ACL) reconstruction (ACLR), with minimal donor site morbidity and failure rates comparable with bone-patellar tendon-bone (BPTB) autograft. This video aims to provide technical tips for ACLR using all-soft-tissue QT autograft. Indications: ACLR with QT autograft is indicated in young, active ACL-injured patients who desire a return to sport. It represents a viable option in both primary and revision ACLR, as well as in skeletally immature patients. It is particularly indicated in those who kneel frequently, such as wrestlers or laborers, due to the lower incidence of postoperative anterior knee pain. Technique Description: A vertical incision is used to harvest a 10 mm × 70 mm partial thickness, all-soft-tissue QT graft. Care is taken not to violate the capsule or musculature. If necessary, graft size is modified based on preoperative magnetic resonance imaging (MRI) measurement of the notch width. The graft is then prepared with continuous loop suspensory fixation on the femoral side and draw sutures on the tibial side. The lateral femoral notch is debrided to allow for visualization of the posterior wall to enable anatomic tunnel placement. When possible, the tibial stump is preserved. The femoral tunnel is drilled via an anteromedial portal and the tibial tunnel via a tibial guide. The graft is then passed through the tibial stump into the femur. It is fixed on the tibial side with a PEEK interference screw in full extension with application of a posterior drawer. Results: Outcomes following ACLR with QT autograft are excellent, with laxity and patient-reported outcomes comparable with those following ACLR with BPTB and hamstring autograft. Furthermore, QT ACLR has been shown to result in less donor site morbidity than BPTB autograft, and lower rates of failure and infection compared with hamstring autograft. Conclusion: ACLR with QT autograft is a good option in young, active patients in both the primary and revision settings. Advantages of QT ACLR include less donor site morbidity than BPTB, and lower rates of failure compared with hamstring autograft in young patients. Patient Consent Disclosure Statement: The author(s) attests that consent has been obtained from any patient(s) appearing in this publication. If the individual may be identifiable, the author(s) has included a statement of release or other written form of approval from the patient(s) with this submission for publication.
Photon-shielding properties of alkali- and acid-treated Philippine natural zeolite
Authors: Gili Mon Bryan Z. 
Published: September 2023

AbstractThe effects of chemical treatment on the radiation-shielding properties of Philippine natural zeolites were investigated using EpiXS following the EPICS2017 library. The zeolites were studied using X-ray diffraction and energy-dispersive X-ray spectroscopy. The acid treatment eliminated Fe and Ca, having a negative impact on the cross-section of the HCl-modified zeolite. The mass attenuation coefficients of the raw, NaOH- and HCl-modified zeolites at 1332 keV were 0.0545, 0.0544 and 0.0548 cm2 g–1, respectively. At 100–10,000 keV, the linear attenuation coefficient depends on the density and increases in the order HCl-modified > NaOH-modified > raw zeolite. In the energy range of 100–16,000 keV, the mean free path and half-value layer values are in the order of HCl-modified < NaOH-modified < raw zeolite. The raw and NaOH-modified zeolites have comparable effective atomic numbers, whereas the HCl-treated zeolite has significantly lower such values.
Chemical and mineralogical reactions of bentonites in geotechnical barriers at elevated temperatures: review of experimental evidence and modelling progress
Authors: Kaufhold Stephan , Dohrmann Reiner , Wallis Ilka , Weber Christian 
Published: September 2023

AbstractBentonites are proposed to be used as buffers in high-level radioactive waste repositories. The elevated temperatures in repositories may, however, affect bentonites’ desired properties. For instance, heating under dry conditions can cause cation fixation, potentially affecting swelling properties. The kinetics of mineral dissolution and precipitation reactions will equally be influenced by temperature. Redistributions of Ca-sulphates and -carbonates have been observed, as well as illitization of smectite. Illitization, however, has only been observed in laboratory experiments at large solution/solid ratios, whereas it has not yet been unambiguously identified in large-scale experiments. In many large-scale tests, cation exchange is the first observable geochemical reaction. In addition, an enrichment of Mg close to the heater is found in many such tests. The thermal gradient and (incongruent) smectite dissolution are suspected to play a role with respect to the Mg enrichment, but the underlying mechanism has not been unravelled so far. To predict the long-term performance of a bentonite buffer, numerical modelling is required in order to be able to simulate the reactions of all accompanying mineral phases. Smectites, which dominate the bentonite composition, are therefore particularly difficult to characterise, as their dissolution is often observed to be non-stoichiometric. Various model approaches exist to simulate smectite reactions, mostly based on kinetic rate reactions, ideally considering the effect of pH (congruent or incongruent dissolution), temperature and the degree of saturation of the solution. Reassessing and improving the thermodynamic/kinetic data of smectites are prerequisites for improving long-term buffer performance assessment.
Theratyping cystic fibrosis patients to guide elexacaftor/tezacaftor/ivacaftor out-of-label prescription
Authors: Dreano Elise , Burgel Pierre Régis , Hatton Aurelie , Bouazza Naim , Chevalier Benoit , Macey Julie , Leroy Sylvie , Durieu Isabelle , Weiss Laurence , Grenet Dominique , Stremler Nathalie , Ohlmann Camille , Reix Philippe , Porzio Michele , Roux Claude Pauline , Rémus Natacha , Douvry Benoit , Montcouquiol Sylvie , Cosson Laure , Mankikian Julie , Languepin Jeanne , Houdouin Veronique , Le Clainche Laurence , Guillaumot Anne , Pouradier Delphine , Tissot Adrien , Priou Pascaline , Mély Laurent , Chedevergne Frederique , Lebourgeois Muriel , Lebihan Jean , Martin Clémence , Zavala Flora , Da Silva Jennifer , Lemonnier Lydie , Kelly-Aubert Mairead , Golec Anita , Foucaud Pierre , Marguet Christophe , Edelman Aleksander , Hinzpeter Alexandre , de Carli Paola , Girodon Emmanuelle , Sermet-Gaudelus Isabelle , Pranke Iwona 
Published: September 2023

BackgroundAround 20% of people with cystic fibrosis (pwCF) do not have access to the triple combination elexacaftor/tezacaftor/ivacaftor (ETI) in Europe because they do not carry the F508del allele on the CF transmembrane conductance regulator (CFTR) gene. Considering that pwCF carrying rare variants may benefit from ETI, including variants already validated by the US Food and Drug Administration (FDA), a compassionate use programme was launched in France. PwCF were invited to undergo a nasal brushing to investigate whether the pharmacological rescue of CFTR activity by ETI in human nasal epithelial cell (HNEC) cultures was predictive of the clinical response.MethodsCFTR activity correction was studied by short-circuit current in HNEC cultures at basal state (dimethyl sulfoxide (DMSO)) and after ETI incubation and expressed as percentage of normal (wild-type (WT)) CFTR activity after sequential addition of forskolin and Inh-172 (ΔIETI/DMSO%WT).Results11 pwCF carried variants eligible for ETI according to the FDA label and 28 carried variants not listed by the FDA. ETI significantly increased CFTR activity of FDA-approvedCFTRvariants (I601F, G85E, S492F, M1101K, R347P, R74W;V201M;D1270N and H1085R). We point out ETI correction of non-FDA-approved variants, including N1303K, R334W, R1066C, Q552P and terminal splicing variants (4374+1G>A and 4096-3C>G). ΔIETI/DMSO%WT was significantly correlated to change in percentage predicted forced expiratory volume in 1 s and sweat chloride concentration (p<0.0001 for both). G85E, R74W;V201M;D1270N, Q552P and M1101K were rescued more efficiently by other CFTR modulator combinations than ETI.ConclusionsPrimary nasal epithelial cells hold promise for expanding the prescription of CFTR modulators in pwCF carrying rare mutants. Additional variants should be discussed for ETI indication.
Acetylcholinesterase inhibition protects against trastuzumab-induced cardiotoxicity through reducing multiple programmed cell death pathways
Authors: Khuanjing Thawatchai , Maneechote Chayodom , Ongnok Benjamin , Prathumsap Nanthip , Arinno Apiwan , Chunchai Titikorn , Arunsak Busarin , Chattipakorn Siriporn C. , Chattipakorn Nipon 
Published: September 2023

Abstract
Background
Trastuzumab (Trz)-induced cardiotoxicity (TIC) is one of the most common adverse effects of targeted anticancer agents. Although oxidative stress, inflammation, mitochondrial dysfunction, apoptosis, and ferroptosis have been identified as potential mechanisms underlying TIC, the roles of pyroptosis and necroptosis under TIC have never been investigated. It has been shown that inhibition of acetylcholinesterase function by using donepezil exerts protective effects in various heart diseases. However, it remains unknown whether donepezil exerts anti-cardiotoxic effects in rats with TIC. We hypothesized that donepezil reduces mitochondrial dysfunction, inflammation, oxidative stress, and cardiomyocyte death, leading to improved left ventricular (LV) function in rats with TIC.

Methods
Male Wistar rats were randomly assigned to be Control or Trz groups (Trz 4 mg/kg/day, 7 days, I.P.). Rats in Trz groups were assigned to be co-treated with either drinking water (Trz group) or donepezil 5 mg/kg/day (Trz + DPZ group) via oral gavage for 7 days. Cardiac function, heart rate variability (HRV), and biochemical parameters were evaluated.

Results
Trz-treated rats had impaired LV function, HRV, mitochondrial function, and increased inflammation and oxidative stress, leading to apoptosis, ferroptosis, and pyroptosis. Donepezil co-treatment effectively decreased those adverse effects of TIC, resulting in improved LV function. An in vitro study revealed that the cytoprotective effects of donepezil were abolished by a muscarinic acetylcholine receptor (mAChR) antagonist.

Conclusions
Donepezil exerted cardioprotection against TIC via attenuating mitochondrial dysfunction, oxidative stress, inflammation, and cardiomyocyte death, leading to improved LV function through mAChR activation. This suggests that donepezil could be a novel intervention strategy in TIC.
Investigation on the Mechanical Properties and Shape Memory Effect of Landing Buffer Structure Based on NiTi Alloy Printing
Authors: Yu Zhenglei , Xin Renlong , Xu Zezhou , Zhu Yining , Zhang Xiaolong , Hao Shijie , Zhang Zhihui , Liang Ping 
Published: September 2023

AbstractWith the deepening of human research on deep space exploration, our research on the soft landing methods of landers has gradually deepened. Adding a buffer and energy-absorbing structure to the leg structure of the lander has become an effective design solution. Based on the energy-absorbing structure of the leg of the interstellar lander, this paper studies the appearance characteristics of the predatory feet of the Odontodactylus scyllarus. The predatory feet of the Odontodactylus scyllarus can not only hit the prey highly when preying, but also can easily withstand the huge counter-impact force. The predatory feet structure of the Odontodactylus scyllarus, like a symmetrical cone, shows excellent rigidity and energy absorption capacity. Inspired by this discovery, we used SLM technology to design and manufacture two nickel-titanium samples, which respectively show high elasticity, shape memory, and get better energy absorption capacity. This research provides an effective way to design and manufacture high-mechanical energy-absorbing buffer structures using bionic 3D printing technology and nickel-titanium alloys.
Characterisation of the Paenarthrobacter nicotinovorans ATCC 49919 genome and identification of several strains harbouring a highly syntenic nic-genes cluster
Authors: El-Sabeh Amada , Mlesnita Andreea-Mihaela , Munteanu Iustin-Tiberius , Honceriu Iasmina , Kallabi Fakhri , Boiangiu Razvan-Stefan , Mihasan Marius 
Published: September 2023

Abstract
Background
Paenarthrobacter nicotinovorans ATCC 49919 uses the pyridine-pathway to degrade nicotine and could provide a renewable source of precursors from nicotine-containing waste as well as a model for studying the molecular evolution of catabolic pathways and their spread by horizontal gene transfer via soil bacterial plasmids.

Results
In the present study, the strain was sequenced using the Illumina NovaSeq 6000 and Oxford Nanopore Technology (ONT) MinION platforms. Following hybrid assembly with Unicycler, the complete genome sequence of the strain was obtained and used as reference for whole-genome-based phylogeny analyses. A total of 64 related genomes were analysed; five Arthrobacter strains showed both digital DNA-DNA hybridization and average nucleotide identity values over the species threshold when compared to P. nicotinovorans ATCC 49919. Five plasmids and two contigs belonging to Arthrobacter and Paenarthrobacter strains were shown to be virtually identical with the pAO1 plasmid of Paenarthrobacter nicotinovorans ATCC 49919. Moreover, a highly syntenic nic-genes cluster was identified on five plasmids, one contig and three chromosomes. The nic-genes cluster contains two major locally collinear blocks that appear to form a putative catabolic transposon. Although the origins of the nic-genes cluster and the putative transposon still elude us, we hypothesise here that the ATCC 49919 strain most probably evolved from Paenarthrobacter sp. YJN-D or a very closely related strain by acquiring the pAO1 megaplasmid and the nicotine degradation pathway.

Conclusions
The data presented here offers another snapshot into the evolution of plasmids harboured by Arthrobacter and Paenarthrobacter species and their role in the spread of metabolic traits by horizontal gene transfer among related soil bacteria.
Sodium tanshinone IIA sulfonate ameliorates neointima by protecting endothelial progenitor cells in diabetic mice
Authors: Heng Yan-Yan , Shang Hui-Juan , Zhang Xia-ze , Wei Wei 
Published: September 2023

Abstract
Background
Endothelial progenitor cells (EPCs) transplantation is one of the effective therapies for neointima associated with endothelial injury. Diabetes impairs the function of EPCs and cumbers neointima prevention of EPC transplantation with an ambiguous mechanism. Sodium Tanshinone IIA Sulfonate (STS) is an endothelium-protective drug but whether STS protects EPCs in diabetes is still unknown.

Methods
EPCs were treated with High Glucose (HG), STS, and Nucleotide-binding Domain-(NOD) like Receptor 3 (NLRP3), caspase-1, the Receptor of Advanced Glycation End products (AGEs) (RAGE) inhibitors, Thioredoxin-Interacting Protein (TXNIP) siRNA, and EPC proliferation, differentiation functions, and senescence were detected. The treated EPCs were transplanted into db/db mice with the wire-injured Common Carotid Artery (CCA), and the CD31 expression and neointima were detected in the CCA inner wall.

Results
We found that STS inhibited HG-induced expression of NLRP3, the production of active caspase-1 (p20) and mature IL-1β, the expression of catalase (CAT) cleavage, γ-H2AX, and p21 in EPCs. STS restored the expression of Ki67, CD31 and von Willebrand Factor (vWF) in EPCs; AGEs were found in the HG-treated EPCs supernatant, and RAGE blocking inhibited the expression of TXNIP and the production of p20, which was mimicked by STS. STS recovered the expression of CD31 in the wire-injured CCA inner wall and the prevention of neointima in diabetic mice with EPCs transplantation.

Conclusion
STS inhibits the aggravated neointima hyperplasia by protecting the proliferation and differentiation functions of EPC and inhibiting EPC senescence in diabetic mice. The mechanism is related to the preservation of CAT activity by inhibiting the RAGE-TXNIP-NLRP3 inflammasome pathway.