bluuebunny/crossref_metadata_2025_split huggingface dataset:
Balancing read length and sequencing depth: Optimizing Nanopore long‐read sequencing for monocots with an emphasis on the Liliales
Authors: De La Cerda Gisel Y. , Landis Jacob B. , Eifler Evan , Hernandez Adriana I. , Li Fay‐Wei , Zhang Jing , Tribble Carrie M. , Karimi Nisa , Chan Patricia , Givnish Thomas , Strickler Susan R. , Specht Chelsea D. 
Published: June 2023

AbstractPremiseWe present approaches used to generate long‐read Nanopore sequencing reads for the Liliales and demonstrate how modifications to standard protocols directly impact read length and total output. The goal is to help those interested in generating long‐read sequencing data determine which steps may be necessary for optimizing output and results.MethodsFour species of Calochortus (Liliaceae) were sequenced. Modifications made to sodium dodecyl sulfate (SDS) extractions and cleanup protocols included grinding with a mortar and pestle, using cut or wide‐bore tips, chloroform cleaning, bead cleaning, eliminating short fragments, and using highly purified DNA.ResultsSteps taken to maximize read length can decrease overall output. Notably, the number of pores in a flow cell is correlated with the overall output, yet we did not see an association between the pore number and the read length or the number of reads produced.DiscussionMany factors contribute to the overall success of a Nanopore sequencing run. We showed the direct impact that several modifications to the DNA extraction and cleaning steps have on the total sequencing output, read size, and number of reads generated. We show a tradeoff between read length and the number of reads and, to a lesser extent, the total sequencing output, all of which are important factors for successful de novo genome assembly.
Lizards and amphisbaenians (Reptilia, Squamata) from the middle Eocene of Mazaterón (Soria, Spain)
Authors: Bolet Arnau 
Published: June 2023

AbstractThe assemblage of lizards and amphisbaenians (Reptilia, Squamata) from the middle Eocene locality of Mazaterón (Spain) is described. Considering the rather limited material available for the study, the assemblage shows a moderate diversity with eight taxa corresponding to five different families. In most cases the scarcity and fragmentary nature of squamate specimens precludes a precise identification, but provides insights on identity of the groups represented. Mazaterón fills the gap between early and late Eocene Iberian localities, showing the persistence of iguanids (possibly Geiseltaliellus), lacertids (possibly Dormaalisaurus), and glyptosaur (tribes glyptosaurini and “melanosaurini”) and anguine anguids through most of the Iberian Eocene. It also records the return of amphisbaenians (Blanidae) after their temporary retrieval from Europe during most of the middle Eocene, and the presence of two scincids, one of them possibly corresponding to a new taxon. The information provided by squamates complements what is already known from mammals, crocodylians, and turtles in what is arguably one of the most important vertebrate Paleogene localities of the Iberian Peninsula.
Is googling risky? A study on risk perception and experiences of adverse consequences in web search
Authors: Häußler Helena , Schultheiß Sebastian , Lewandowski Dirk 
Published: June 2023

AbstractSearch engines, such as Google, have a considerable impact on society. Therefore, undesirable consequences, such as retrieving incorrect search results, pose a risk to users. Although previous research has reported the adverse outcomes of web search, little is known about how search engine users evaluate those outcomes. In this study, we show which aspects of web search are perceived as risky using a sample (N = 3884) representative of the German Internet population. We found that many participants are often concerned with adverse consequences immediately appearing on the search engine result page. For example, 45.2% of respondents are concerned about retrieving incorrect information. In contrast, consequences with a delayed impact are rarely perceived as a risk. Moreover, participants' experiences with adverse consequences are directly related to their risk perception. Our results demonstrate that people perceive risks related to web search. In addition to our study, there is a need for more independent research on the possible detrimental outcomes of web search to monitor and mitigate risks. Apart from risks for individuals, search engines with a massive number of users have an extraordinary impact on society; therefore, the acceptable risks of web search should be discussed.
Emerging nonoxide dielectrics for next‐generation electronics
Authors: Kim Ga Hye , Shin Seung Beom , Kim Myung‐Gil 
Published: June 2023

AbstractAs electronic devices continue to develop, there is a growing demand for materials capable of supporting new physical properties, such as flexibility, stretchability, low‐temperature processability, applicability to large areas or ultra‐thin films, and operation at extremely low voltages. Metal oxide dielectrics, such as silicon dioxide, aluminum oxide, and hafnium oxide, offer excellent dielectric properties, but their ability to meet these new properties and the choice of substrate is limited. Therefore, a new dielectric material capable of providing stable dielectric properties with new functionalities is required. In this review, we briefly introduce the physical principles of dielectric materials and the recent trends of nonoxide dielectrics which satisfy requirements of new functionalities. We discussed various nonoxide dielectric materials of organic, self‐assembled mono/multilayers, electrolytes, two‐dimensional (2D) materials, and metal halides. Finally, we conclude with an outlook and challenges on dielectric materials for next‐generation micro‐ and macro‐electronics.
White matter abnormalities are associated with the declined ability of reasoning and problem‐solving in major depressive disorder
Authors: Ma Ruihua , Luo Yang , Liu Sijia , Wang Xuan , Guo Hua , Zhao Meng , Chen Nan , Liu Panqi , Shi Jing , Li Yingna , Tan Yunlong , Tan Shuping , Yang Fude , Tian Li , Wang Zhiren 
Published: June 2023

AbstractObjectivesExecutive function in people with depression is linked to the integrity of white matter fibers in the brain. We hypothesized that the maze tests in neuropsychological tests assessed reasoning and problem‐solving abilities dependent on the integrity of brain white matter fibers, and assessed this relationship using diffusion tensor imaging (DTI) in depressed patients and healthy controls.MethodsParticipants aged from 18 to 50 years were recruited from Zhumadian Second People's Hospital from July 2018 to August 2019. The sample included 33 clinically diagnosed individuals with major depressive disorder (MDD) and 24 healthy volunteers (HVs). All subjects underwent Neuropsychological assessment battery (NAB) maze tests and DTI. Tract‐based spatial statistics technology in FSL software was used to process DTI data, and threshold‐free cluster enhancement (TFCE) was used to perform multiple comparison corrections. The fractional anisotropy (FA) of white matter fibers in the MDD group and HVs group were compared and extracted. Pearson correlation was used to analyze the relationship between FA and NAB scores and HAMD scores.ResultsThe mean NAB maze test score for the MDD group was lower than the HVs group, and the difference was statistically significant (F = 11.265, p = .037). The FA value of the body of corpus callosum and cerebral peduncle right in the depression group was lower than that in the healthy control group, and the difference was statistically significant (p < .05). FA value of the body of corpus callosum was positively correlated with NAB score (r = 0.400, p = .036), but not with the HAMD score (r = 0.065, p = .723).ConclusionsThe decreased ability of reasoning and problem‐solving in MDD may be due to the decreased integrity of the white matter fibers of the body of the corpus callosum.
Development of software for measuring brain amyloid accumulation using 18F‐florbetapir PET and calculating global Centiloid scale and regional Z‐score values
Authors: Matsuda Hiroshi , Soma Tsutomu , Okita Kyoji , Shigemoto Yoko , Sato Noriko 
Published: June 2023

AbstractBackground and purposeQuantitative measures have been proposed to aid the visual interpretation of amyloid PET. Our objective was to develop and validate quantitative software that enables calculation of the Centiloid (CL) scale and Z‐score for amyloid PET with 18F‐florbetapir.MethodsThis software was developed as a toolbox in statistical parametric mapping 12 running on MATLAB Runtime. For each participant's amyloid PET, this software calculates the CL scale using the standard MRI‐guided pipeline proposed by the Global Alzheimer's Association Interactive Network (GAAIN) and generates a Z‐score map for comparison with a new amyloid‐negative database constructed from 20 healthy controls. In 23 cognitively impaired patients with suspected Alzheimer's disease, Z‐score values for a target cortical area from the new database were compared with those from the GAAIN database constructed from 13 healthy controls. The CL values obtained using low‐dose CT of PET/CT equipment were then compared with those obtained using MRI.ResultsThe CL calculation was validated with the 18F‐florbetapir dataset in the GAAIN repository. Z‐score values obtained from the new database were significantly higher (mean ± standard deviation, 1.05 ± 0.77; p < .0001) than those obtained from the GAAIN database. The use of low‐dose CT provided CL scales that were highly correlated with those obtained with MRI (R2 = .992) but showed a slight yet significant underestimation (−2.1 ± 4.2; p = .013).ConclusionsOur quantification software provides the CL scale and Z‐score for measuring overall and local amyloid accumulation with the use of MRI or low‐dose CT.
Development and psychometric evaluation of the family intensive care units syndrome inventory
Authors: Saeid Yaser , Ebadi Abbas , Salaree Mohammad Mahdi , Moradian Seyed Tayeb 
Published: June 2023

AbstractBackgroundFamily members of patient in the intensive care unit (ICU) experience a set of problems which are entitled Family Intensive Care Units Syndrome (FICUS).ObjectivesThe aim of this study was to develop and psychometrically evaluate the FICUS Inventory (FICUSI) in Iran.MethodsThis sequential exploratory mixed method study was conducted in 2020 in two main phases. In the first phase, FICUSI was developed based on the results of an integrative review and a qualitative study. In the second phase, the psychometric properties of FICUSI, namely, face, content, and construct validity, reliability, responsiveness, interpretability, and scoring, were evaluated. The sample for the construct validity evaluation consisted of 283 ICU family members.ResultsThe primary item pool of FICUSI had 144 items and was reduced to 65 items or omitting overlapping and similar items. The scale‐level content validity index of FICUSI was 0.89. In the construct validity evaluation through exploratory factor analysis, 31 items with factor loading values more than 0.3 were loaded on two factors (namely psychological symptoms and nonpsychological symptoms) which explained 68.45% of the total variance. The Cronbach's alpha and the test‐retest intraclass correlation coefficient of FICUSI were 0.95 and 0.97, respectively.ConclusionFICUSI is a valid and reliable instrument which can be used in clinical settings and studies for FICUS assessment. Further studies for the cross‐cultural adaptation of FICUSI in other contexts are recommended.Relevance to clinical practiceHealth care providers in clinical settings can use FICUSI to assess FICUS among the family caregivers of patients in ICU. Health care providers’ better understanding of FICUS helps them understand the quality of their own services for the family members of patients in ICU.
Lesion patterns and mechanism analysis of acute contralateral ischemic stroke accompanying stenosis of unilateral extracranial internal carotid artery
Authors: Li Mengxin , Pang Meng , Cui Xiaomei , Wang Yuge , Jia Shuai , Lu Zhengqi , Wang Yanqiang 
Published: June 2023

AbstractBackgroundPrevious studies on unilateral internal carotid artery occlusive disease have focused on the mechanisms of ipsilateral hemispheric stroke, and contralateral stroke is considered to be an accidental phenomenon. Little is known about the relationship between severe stenosis (including occlusion) of the unilateral extracranial segment of the internal carotid artery and contralateral cerebral stroke, and the infarct patterns and pathogenesis require further study. The purpose of this study was to investigate the clinical characteristics and pathogenesis of contralateral acute stroke with unilateral extracranial internal carotid artery stenosis (including occlusion).MethodsThirty‐four patients were enrolled in this study, and all patients underwent routine clinical evaluation, including medical history, physical examination, laboratory tests, and various imaging evaluations. The morphological characteristics of diffusion‐weighted magnetic resonance imaging were applied to determine infarct patterns. The etiological classification was confirmed according to the TOAST classification.ResultsThere were six distinctive lesion patterns: small subcortical infarcts (six patients), large subcortical infarcts (one patient), diffuse infarcts (eight patients), multiple anterior circulation infarcts (eight patients), multiple posterior circulation infarcts (two patients), and multiple anterior and posterior circulation infarcts (nine patients).ConclusionDiffuse and multiple infarcts were the most common topographic patterns in ischemic stroke contralateral to internal carotid artery stenosis or occlusion. Hemodynamic impairment of the contralateral hemisphere due to hypoperfusion and blood theft is regarded as the basis of stroke occurrence. Low ischemic tolerance and embolism are the main causes of acute ischemic stroke.
Salivary micro RNAs as biomarkers for oropharyngeal cancer
Authors: Ekanayake Weeramange Chameera , Tang Kai Dun , Barrero Roberto A. , Hartel Gunter , Liu Zhen , Ladwa Rahul , Langton‐Lockton Julian , Frazer Ian , Kenny Lizbeth , Vasani Sarju , Punyadeera Chamindie 
Published: June 2023

AbstractBackgroundDespite the rising incidence, particularly of the human papillomavirus (HPV)‐associated fraction of oropharyngeal cancer (OPC), there are no early detection methods for OPC. Considering the close association between saliva and head and neck cancers, this study was designed to investigate salivary micro RNA (miRNAs) associated with OPC, especially focusing on HPV‐positive OPC.MethodsSaliva was collected from OPC patients at diagnosis and patients were clinically followed up ≤5 years. Salivary small RNA isolated from HPV‐positive OPC patients (N = 6), and HPV‐positive (N = 4) and negative controls (N = 6) were analysed by next‐generation sequencing to identify dysregulated miRNAs. Discovered miRNAs were validated by quantitative PCR using two different assays in a separate cohort of patients (OPC = 91, controls = 92). The relative expression was calculated considering SNORD‐96A as the normalizer. Candidate miRNAs with diagnostic and prognostic potential were evaluated by generalized logistic regression.ResultsA panel consisting of nine miRNAs was identified to have the best diagnostic performance to discriminate HPV‐positive OPC from HPV‐positive controls (AUC‐ validation‐1 = 94.8%, validation‐2 = 98%). Further, a panel consisting of six miRNAs were identified to discriminate OPC from controls regardless of the HPV status (AUC‐ validation‐1 = 77.2%, validation‐2 = 86.7%). In addition, the downregulation of hsa‐miR‐7‐5p was significantly associated with poor overall survival of OPC patients (HR = 0.638). A panel consisting of nine miRNAs were identified for the prediction of the overall survival of the OPC patients (log‐rank test‐p = 0.0008).ConclusionThis study highlights that salivary miRNAs can play an essential role in the detection and prognostication of OPC.
Magnetic resonance imaging characteristics of brain metastases in small cell lung cancer
Authors: Zhu Dan , Shao Yongjia , Yang Zhangwei , Cheng Ailan , Xi Qian , Liang Xiaohua , Chu Shuguang 
Published: June 2023

AbstractBackgroundLung is the most common primary site of brain metastases (BMs). For different pathological types of BMs have some similar characteristics, it is still a challenge to confirm the origin based on their characteristics directly. BMs of small cell lung cancer (SCLC) have favorable therapeutic expectations due to their high sensitivity to radiotherapy. This study sought to identify unique characteristics of BMs in SCLC, aiming to assist in clinical decision‐making.MethodsPatients diagnosed with BMs of lung cancer who received radiotherapy from January 2017 to January 2022 were reviewed (N = 284). Definitive diagnosis of BMs of SCLC was reached for 36 patients. All patients underwent head examination using magnetic resonance imaging. The number, size, location, and signal characteristics of lesions were analyzed.ResultsThere were 7 and 29 patients with single focus and non‐single focus, respectively. Ten patients had diffuse lesions, and the remaining 26 patients had a total of 90 lesions. These lesions were divided into three groups according to size: <1, 1–3, and >3 cm (43.33%, 53.34%, and 3.33%, respectively). Sixty‐six lesions were located in the supratentorial area, primarily including cortical and subcortical lesions (55.56%) and deep brain lesions (20%). Moreover, 22 lesions were located in the infratentorial area. According to diffusion‐weighted imaging and T1‐weighted contrast enhancement, the imaging characteristics were classified into six patterns. Hyperintensity in diffusion‐weighted imaging and homogeneous enhancement was the most common pattern of BMs in SCLC (46.67%), while partial lesions showed hyperintensity in diffusion‐weighted imaging without enhancement (7.78%).ConclusionsThe manifestations of BMs in SCLC were multiple lesions (diameter: 1–3 cm), hyperintensity in diffusion‐weighted imaging, and homogeneous enhancement. Interestingly, hyperintensity in diffusion‐weighted imaging without enhancement was also one of the characteristics.
Customer experience in coffee stores: A multidisciplinary Neuromarketing approach
Authors: Rancati Gaia , Nguyen Thi Thu Thao , Fowler Danae , Mauri Maurizio , Schultz Carsten D. 
Published: June 2023

AbstractCompanies in the service retailing industry are constantly looking for strategies to provide a customer experience that improves brand perception and increases customer loyalty. This study provides a deeper understanding of customer experience in two coffee stores in Vietnam by providing a multidisciplinary approach, including a quantitative and a neuromarketing approach. Two‐hundred participants participated in the study, combining mystery shopping visits with the Brand Association Reaction Time Test (BARTT). Results from the mystery shopping visits confirm the holistic concept of customer experience. Store atmosphere, employee service, drink quality, price, and previous visits significantly impact customer experience. Empirical results also confirm the impact of customer experience on loyalty. Furthermore, the BARTT analysis shows that in‐store customer experience can influence customers' short‐term brand perceptions. The national brand is associated with friendly, expensive, globalized, and unique, whereas the local brand revealed significant associations with clean, smells good, welcoming, and unique. From the mystery shopping results and the BARTT, we can infer that customer experience needs multi‐methodological capturing of its multifaceted aspects. Our findings are relevant to researchers and practitioners seeking to understand and bolster customer experiences, justifying a multidisciplinary approach and the application of new insightful tools.
Control Strategies for Enhanced Biogas Production from Chicken Manure
Authors: Siyal Ahmer Ali , Shamsuddin Rashid , Komiyama Masaharu , Manogaran M. Devendran , Soehartanto Totok 
Published: June 2023

AbstractThe control strategies adopted in formulation and anaerobic digestion (AD) process of chicken manure (CM) for the control and enhancement of biogas production are reviewed. The existing limitations and future challenges in the production of biogas from AD of CM are briefly discussed. The control strategy adopted for formulation is pretreatment of CM (physical, chemical, biological, and thermal) while strategies for AD include selection of optimal parameters (pH, C/N ratio, organic loading rate (OLR), and temperature), co‐AD with other substrates, and addition of supporting materials. The pretreatment of CM, selection of optimal parameters, Co‐AD of CM with other substrates, and addition of supporting materials overall enhance biogas and methane production due to increase of hydrolysis and C/N and decrease of ammonia inhibition, CO2 and H2S contents. The implementation of developed control strategies in continuous plants, development of new strategies, and use of supporting materials in combinations of two or three for better performance are the future challenges in the AD of CM. The control strategies had a significant impact on the efficient use of CM and improved biogas and methane production.
Efficient determination of pesticides in rice by QuEChERS and UV spectroscopy in combination with chemometrics
Authors: Wan Yuhang , Yuan Xinyu , Liu Jia , Zhang Ding , Zhang Zhengyong , Sha Min 
Published: June 2023

AbstractBackground and ObjectivesTargeting the problems, like various residual pesticides, the high interference of sample matrix and the slow detection speed, this work studied the rapid screening method of multipesticide residues in rice by taking bensulfuron‐methyl, propanil and cypermethrin as examples.FindingsRice matrix solution was obtained by using the improved QuEChERS method, matrix standard solution, and solvent standard solution were prepared to obtain the linear range and matrix effect of each pesticide. QuEChERS method could rapidly pretreat samples, and the matrix effect was soft, with matrix effect values lower than 16%. Mixed solutions of the three pesticides in four combinations were prepared within the linear range. The type of pesticides in rice was determined by support vector machine (SVM), and the pesticide concentration was predicted by partial least square regression (PLSR). SVM provided a supervised identification method for pesticide residues, making the recognition accuracy higher than 90%. The error of PLSR in predicting pesticide concentration was lower than 5.36%.ConclusionsUV spectroscopy had fast test speed, so the combination of QuEChERS and UV spectroscopy could improve the efficiency of analysis. SVM combined with PLSR could preliminarily determine the type of pesticides in rice sample, and then accurately predict the concentration of pesticides.Significance and NoveltyThe QuEChERS sample pretreatment technology and UV spectroscopy in combination with chemometrics was expected to be a new technology for rapid detection of pesticide residues in samples.
Visual impairment following aluminum phosphide poisoning: A rare case
Authors: Kargar Alireza , Noroozian Maryam , Ramezani Mahtab , Shadnia Shahin , Mostafazadeh Babak , Erfan Talab Evini Peyman , Rahimi Mitra 
Published: June 2023

Key Clinical MessageAluminum phosphide poisoning may cause rare visual impairment. In a case, a 31‐year‐old female, visual loss was linked to shock‐induced hypoperfusion, causing oxygen lack and cerebral atrophy, emphasizing the need for identifying atypical symptoms.AbstractThis case report describes the multidisciplinary evaluation of a 31‐year‐old female patient who suffered from visual impairment as a result of aluminum phosphide (AlP) poisoning. Phosphine, which is formed in the body when AlP reacts with water, cannot cross the blood–brain barrier; therefore, visual impairment seems unlikely to be the direct result of phosphine. To our knowledge, it is the first documented report of such impairment due to AlP.