bluuebunny/crossref_metadata_2025_split huggingface dataset:
Chemical Composition, Anthelmintic Activity, and Mechanism of Action of Lippia dominguensis Mold. Essential Oil on Haemonchus contortus
Authors: Espino Ureña Marcos J. , Katchborian‐Neto Albert , Trinidad Alexander Benítez , Ramírez Ramírez Maritza , Vásquez Tineo Manuel , Vilemar de Araújo‐Filho José , Ribeiro Wesley Lyeverton Correia , de Souza Collares Maia Castelo‐Branco Débora , de Oliveira Lorena Mayana Beserra , Bevilaqua Claudia Maria Leal , Chagas‐Paula Daniela Aparecida , Viegas Claudio , Marques Marcos José 
Published: May 2023

AbstractGastrointestinal nematode parasitism is a major burden to small ruminant production globally, compounded by increasing anthelmintic resistance. Previous studies have identified essential oils (EOs) from the Lippia genus with antiprotozoal and anthelmintic effects. Lippia dominguensis Moldenke (Ld), an endemic specie from the Dominican Republic, has similar popular uses, however, is chemically and pharmacologically yet uncharacterized. Here, we investigated the in vitro anthelmintic activity of LdEO and its ultrastructural effects on eggs and adult nematodes of Haemonchus contortus multidrug‐resistant isolated. The GC/MS analysis showed linalool (33.85 %), 1,8‐cineole (30.88 %), and δ‐terpineol (10.61 %) as the main EO constituents. The LdEO showed an IC50=0.523 mg/mL in the egg hatch test, and the motility in the adult worm motility test was 95.8 % at 1 mg/mL. The confocal scanning laser microscopy of eggs indicated permeabilization or disruption of egg cell membranes as the possible mechanism of action of LdEO. The scanning electron microscopy of adult worms showed wrinkling, undulations, and cuticular disruptions. The LdEO displayed significant in vitro anthelmintic activity on eggs and adult worms of H. contortus. Additionally, the LdEO showed low oral toxicity in mice at 2,000 mg/kg. Thus, additional in vivo studies are justified to determine its anthelmintic efficacy in small ruminants.
Increased cytokine levels induced by high phenylalanine concentrations in late diagnosis PKU patients compared to early diagnosis: Anti‐inflammatory effect of L‐carnitine
Authors: Faverzani Jéssica Lamberty , Hammerschmidt Tatiane Grazieli , Mescka Caroline Paula , Guerreiro Gilian , Lopes Franciele Fatima , Delgado Camila Aguilar , de Moura Coelho Daniella , Sitta Angela , Deon Marion , Wajner Moacir , Vargas Carmen Regla 
Published: May 2023

AbstractPhenylketonuria (PKU) was the first genetic disease to have an effective therapy, which consists of phenylalanine intake restriction. However, there are patients who do not adhere to treatment and/or are not submitted to neonatal screening. PKU patients present L‐carnitine (L‐car) deficiency, compound that has demonstrated an antioxidant and anti‐inflammatory role in metabolic diseases. This study evaluated the effect caused by exposure time to high Phe levels in PKU patients at early and late diagnosis, through pro‐ and anti‐inflammatory cytokines, as well as the L‐car effect in patients under treatment. It was observed that there was a decrease in phenylalanine levels in treated patients compared to patients at diagnosis, and an increase in L‐car levels in the patients under treatment. Inverse correlation between Phe versus L‐car and nitrate plus nitrite versus L‐car in PKU patients was also showed. We found increased proinflammatory cytokines levels: interleukin (IL)‐1β, interferons (IFN)‐gamma, IL‐2, tumor necrosis factor (TNF)‐alpha, IL‐8 and IL‐6 in the patients at late diagnosis compared to controls, and IL‐8 in the patients at early diagnosis and treatment compared to controls. Increased IL‐2, TNF‐alpha, IL‐6 levels in the patients at late diagnosis compared to early diagnosis were shown, and reduced IL‐6 levels in the treated patients compared to patients at late diagnosis. Moreover, it verified a negative correlation between IFN‐gamma and L‐car in treated patients. Otherwise, it was observed that there were increased IL‐4 levels in the patients at late diagnosis compared to early diagnosis, and reduction in treated patients compared to late diagnosed patients. In urine, there was an increase in 8‐isoprostane levels in the patients at diagnosis compared to controls and a decrease in oxidized guanine species in the treated patients compared to the diagnosed patients. Our results demonstrate for the first time in literature that time exposure to high Phe concentrations generates a proinflammatory status, especially in PKU patients with late diagnosis. A pro‐oxidant status was verified in not treated PKU patients. Our results demonstrate the importance of early diagnosis and prompt start of treatment, in addition to the importance of L‐car supplementation, which can improve cellular defense against inflammation and oxidative damage in PKU patients.
Expression and Characterization of a New Self‐Sufficient P450 Monooxygenase (P450 AZC1) from Azorhizobium caulinodans
Authors: Yıldırım Didem , Ozic Cem , Ensari Yunus 
Published: May 2023

AbstractOxyfunctionalization of non‐activated carbon bonds by P450 monooxygenases has drawn great industrial attraction. Self‐sufficient P450s containing catalytic heme and reductase domains in a single polypeptide chain offer many advantages since they do not require external electron transfer partners. Here, we report the first P450 enzyme identified and expressed from Azorhizobium caulinodans. Firstly, expression conditions of P450 AZC1 were optimized for enhanced expression in E.coli. The highest P450 content was obtained in E.coli Rosetta DE3 plysS when it was incubated in TB media supplemented with 0.75 mM IPTG, 0.5 mM ALA, and 0.75 mM FeCl3 at 25 °C for 24 hours. Subsequently, the purified enzyme showed a broad substrate spectrum including fatty acids, linear and cyclic alkanes, aromatics, and pharmaceuticals. Finally, P450 AZC1 showed optimal activity at pH 6.0 and 40 °C and a broad pH and temperature profile, making it a promising candidate for industrial applications.
Jointly Depleting Glutathione Based on Self‐Assembled Nanomicelles for Enhancing Photodynamic Therapy
Authors: Wu Jingxi , Shang Jingjing , An Jing , Chen Wenlong , Hong Gaobo , Hou Haoran , Zheng Wen‐Heng , Song Fengling , Peng Xiaojun 
Published: May 2023

AbstractPhotodynamic therapy (PDT) is one common ROS‐generating therapeutic method with high tumor selectivity and low side effects. But the GSH‐upregulation often alleviates its therapeutic efficiency. Here, we proposed a new strategy of jointly depleting GSH to enhance the therapeutic effect of PDT by preparing a nanomicelle by self‐assembly method from GSH‐activated photosensitizer DMT, curcumin, and amphiphilic polymer TPGS.
The contemporary role of protamine in the cardiac catheterization laboratory
Authors: Danek Barbara A. , Kearney Kathleen E. , Chung Christine J. , Steinberg Zachary , Lombardi William L. , McCabe James M. , Azzalini Lorenzo 
Published: May 2023

AbstractAccess to the arterial circulation and full anticoagulation carries a risk of serious bleeding during and after percutaneous coronary intervention. Important sources of bleeding include the arterial access site and coronary artery perforation. Prompt and effective management of hemorrhagic complications is an essential interventional skill. Protamine sulfate is well‐known as a heparin reversal agent. Despite this, there is heterogeneity in the use of protamine during interventional procedures. While protamine is generally well‐tolerated, it is associated with a risk of hypersensitivity reaction, including anaphylaxis, among others. The purpose of this review is to summarize the existing evidence about and experience with the use of protamine sulfate in the setting of percutaneous coronary and structural interventional procedures.
Determining the impact of genotype × environment on oat protein isolate structural and functional characteristics
Authors: Mel Roshema , Gough Kathleen M. , Nilsen Kirby T. , Beattie Aaron D. , Malalgoda Maneka 
Published: May 2023

AbstractBackground and ObjectivesThe effect of genotype × environment (G × E) on oat protein structural characteristics and functionality was studied using three oat genotypes grown in three different environments across the Canadian Prairies.FindingsThe protein content of oat protein isolate (OPI) ranged from 76.9% to 90.2%. All structural characteristics except protein surface hydrophobicity were significantly impacted by G × E, whereas protein surface hydrophobicity strongly depended on the growing environment. SDS‐PAGE and FT‐IR analysis demonstrated that oat protein profile and secondary structure were significantly impacted by genotype rather than environment. Cultivar Summit grown in Alberta had the highest foaming capacity which was significantly higher than Summit grown in the other environments, indicating a strong influence of environment on OPI foaming capacity.ConclusionThe structure‐function properties of OPI extracted from the given cultivars were influenced by G × E however, further studies are needed to evaluate the stability of the tested cultivars over several years.Significance and NoveltyUnderstanding how OPI structure and functional properties are influenced by G × E will allow better utilization of OPI in the food industry by facilitating the development of OPI ingredients with predictable functional properties.
Origin of the Captodative Effect: The Lone‐Pair Shielded Radical
Authors: Blokker Eva , ten Brink Martijn , van der Schuur J. Martijn , Hamlin Trevor A. , Bickelhaupt F. Matthias 
Published: May 2023

AbstractWe have quantum chemically analyzed the origin of the captodative effect in the dimerization ofpara‐substituted phenyl dicyanomethyl radicals RPh(CN)2C⋅ in the gas phase and in solution. Captodative radicals are characterized by the presence of both, electron‐donating and electron‐withdrawing groups, and a weakening of the associated C−C bond in the dimer of these radicals. Our quantitative bonding analyses reveal that the captodative weakening of the C−C bond is the consequence of a special feature in the RPh(CN)2C⋅ electronic structure which we designate “lone‐pair shielded radical”. Solvation effects weaken the C−C bond as the radicals have a more prominent internal charge separation than the dimer and are, therefore, stabilized more than the intact dimer. Interestingly, we find that differences in solvent effects as a function of thepara‐substituent in the most prominent case arise from variations in the charge distribution in the dimer, not from that in the separate radicals which experience very similar solvation in those instances.
Bismuth Cations: Fluoride Ion Abstraction, Isocyanide Coordination, and Impact of Steric Bulk on Lewis Acidity
Authors: Dunaj Tobias , Schwarzmann Johannes , Ramler Jacqueline , Stoy Andreas , Reith Sascha , Nitzsche Joel , Völlinger Lena , von Hänisch Carsten , Lichtenberg Crispin 
Published: May 2023

AbstractInvited for the cover of this issue are the groups of Carsten von Hänisch and Crispin Lichtenberg at the Philipps University of Marburg. The image depicts a bismuth kraken, eagerly grabbing Lewis basic substrates, thereby solving scientific puzzles about bismuth‐based Lewis acidity. Read the full text of the article at 10.1002/chem.202204012.
Ultra wideband (UWB) dielectric resonator antenna using fractal‐inspired feeding mechanism
Authors: Huda Shabana , Saha Anuradha , Karmakar Anirban 
Published: May 2023

SummaryThis article examines a compact dielectric resonator antenna (DRA) for UWB applications. Here a composite feeding structure excites a dielectric resonator which in tern provides the resonant modes TE111, TE121, TE212, and TE222. The dielectric resonator (DR), built of alumina ceramic (εr, DR = 9.8), is mounted on a fractal triangular patch, inspired by Sierpinski Gasket. The suggested DRA is supported by a FR4 substrate (εr, sub = 4.4) and measures compactly 40 × 30 × 8.5 mm3. To confirm the results of its simulations, the proposed antenna's prototype is prepared and measured up to the second iteration. The measured outcome demonstrates that the suggested antenna has a frequency range of 3.38–10.71 GHz (104%) for S11 < −10 dB and provides a maximum gain of 7.23 dBi at 8 GHz along with highest possible simulated efficiency of 98%. The suggested DRA is appropriate for small‐range future 4G/5G UWB wireless multimedia applications due to its ultrawide bandwidth, excellent gain, reasonable efficiency, omnidirectional radiation features, and compact construction. This DRA is also suitable for repeaters of mobile and vehicular communications.
Inosine attenuates rotenone‐induced Parkinson's disease in rats by alleviating the imbalance between autophagy and apoptosis
Authors: El‐Latif Aya M. Abd , Rabie Mostafa A. , Sayed Rabab H. , Fattah Mai A. Abd El , Kenawy Sanaa A. 
Published: May 2023

AbstractGrowing evidence points to impaired autophagy as one of the major factors implicated in the pathophysiology of Parkinson's disease (PD). Autophagy is a downstream target of adenosine monophosphate‐activated protein kinase (AMPK). Inosine has already demonstrated a neuroprotective effect against neuronal loss in neurodegenerative diseases, mainly due its anti‐inflammatory and antioxidant properties. We, herein, aimed at investigating the neuroprotective effects of inosine against rotenone‐induced PD in rats and to focus on the activation of AMPK‐mediated autophagy. Inosine successfully increased p‐AMPK/AMPK ratio in PD rats and improved their motor performance and muscular co‐ordination (assessed by rotarod, open field, and grip strength tests, as well as by manual gait analysis). Furthermore, inosine was able to mitigate the rotenone‐induced histopathological alterations and to restore the tyrosine hydroxylase immunoreactivity in PD rats' substantia nigra. Inosine‐induced AMPK activation resulted in an autophagy enhancement, as demonstrated by the increased striatal Unc‐S1‐like kinase1 and beclin‐1 expression, and also by the increment light chain 3II to light chain 3I ratio, along with the decline in striatal mammalian target of rapamycin and p62 protein expressions. The inosine‐induced stimulation of AMPK also attenuated neuronal apoptosis and promoted antioxidant activity. Unsurprisingly, these neuroprotective effects were antagonized by a preadministration of dorsomorphin (an AMPK inhibitor). In conclusion, inosine exerted neuroprotective effects against the rotenone‐induced neuronal loss via an AMPK activation and through the restoration of the imbalance between autophagy and apoptosis. These findings support potential application of inosine in PD treatment.
Deconvolution approach for floating wind turbines
Authors: Liu Zirui , Gaidai Oleg , Sun Jiayao , Xing Yihan 
Published: May 2023

AbstractGreen renewable energy is produced by floating offshore wind turbines (FOWT), a crucial component of the modern offshore wind energy industry. It is a safety concern to accurately evaluate excessive weights while the FOWT operates in adverse weather conditions. Under certain water conditions, dangerous structural bending moments may result in operational concerns. Using commercial FAST software, the study's hydrodynamic ambient wave loads were calculated and converted into FOWT structural loads. This article suggests a Monte Carlo‐based engineering technique that, depending on simulations or observations, is computationally effective for predicting extreme statistics of either the load or the response process. The innovative deconvolution technique has been thoroughly explained. The suggested approach effectively uses the entire set of data to produce a clear but accurate estimate for severe response values and fatigue life. In this study, estimated extreme values obtained using a novel deconvolution approach were compared to identical values produced using the modified Weibull technique. It is expected that the enhanced new de‐convolution methodology may offer a dependable and correct forecast of severe structural loads based on the overall performance of the advised de‐convolution approach due to environmental wave loading.
Viscoelastic boundary layer analysis of constant surface temperature plate embedded in saturated porous media
Authors: Elayyan Mutaz , Maaitah Hussein , Quran Omar , Awad Ahmad S. , Duwairi Hamzeh M. 
Published: May 2023

AbstractMathematical models and numerical solutions of Williamson fluid flow under influences of various boundary conditions provide important support to experimental studies in the solar energy field. Therefore, the present study is concerned with the effects of forced convection of the viscoelastic boundary layer on a horizontal plate embedded in saturated porous media subjected to constant surface temperature. The study explores the profiles of shear stress, velocity, temperature, and heat transfer coefficient. The governing equations in nondimensional forms are obtained by using a model of Darcy–Forchheimer–Brinkman and finally are solved numerically by using bvp4c with MATLAB package. The results of the numerical solution show an insignificant rise in the distribution of the velocity boundary layer and shear stress profile as the Darcy parameter is increased, while a decrease in the temperature and Nusselt numbers are found. On the other hand, as the viscoelastic parameter is increased, the Darcy parameter shows a reverse response. Finally, insignificant increases in profiles of boundary layer velocity, temperature, shear stress, and Nusselt number are observed at high values of the Forchheimer number.
Intraoperative ultrasound assessment of ureteral patency after uterosacral ligaments apical suspension for prolapse surgical repair: A feasibility study
Authors: Frigerio Matteo , Barba Marta , Marino Giuseppe , Volontè Silvia , Cola Alice 
Published: May 2023

AbstractObjectiveTo prospectively compare power Doppler ultrasound diagnostic performance with reference standard cystoscopy in evaluating ureteral patency in a population at high risk of ureteral lesions.MethodsWe analyzed 100 women who underwent pelvic organ prolapse repair. All ultrasound scans were obtained, at the end of the procedures, before cystoscopic evaluation. Bilateral simultaneous ureteral jet evaluation with power Doppler was performed at the level of the ureterovesical junctions with a pulse repetition frequency set to detect low flow for a maximum of 3 min.ResultsAccording to the reference standard urethrocystoscopy, at least one ureter not ejaculating was observed in 6% of patients, for a total of seven ureters jets not visualized. No false‐negative results were obtained. Ultrasound with power Doppler showed 100% sensitivity and 95.9% specificity in detecting the lack of ureteral jet. The negative predictive value was 100%, and the positive predictive value was 46.7%.ConclusionsUltrasound with power Doppler represents an effective and reliable non‐invasive screening test to exclude ureteral kinking and reduce the need for intraoperative cystoscopy.
The mitochondrial gene COI reveals the origin of the introduction of Platanichthys platana (Regan, 1917) (Clupeiformes, Clupeidae) in a neotropical floodplain
Authors: Ramos Laura I. , Scorsim Bárbara , Pavanelli Carla S. , de Oliveira Alessandra V. 
Published: May 2023

AbstractThe first records of Platanichthys platana in the upper Rio Paraná floodplain (URPF) date to 2012, but variations in the body length of specimens collected from this basin have since generated uncertainty about their identity and origin of introduction. This study aimed to genetically characterize populations of P. platana in different river basins, assess haplotype diversity, and determine the origin of the introduced population in the URPF. Sequences from the COI region were used to calculate genetic distances, construct gene trees, and identify haplotype networks. Eight haplotypes were found, several of which were exclusive to the coastal basins of Santa Catarina and Rio Grande do Sul, and the Río Negro basin (Río Yí). The Río de La Plata and Mar Chiquita basins, and upper Rio Paraná floodplain shared haplotypes. Based on the results of our analysis, we concluded that the URPF specimens were introduced from populations in Argentina.
Feeder cattle genomic tests: Analyzing cattle producer adoption decisions
Authors: DeLong Karen L. , Jensen Kimberly L. , Griffith Andrew P. , Boyer Christopher N. , Martinez Charley C. 
Published: May 2023

AbstractGenomic tests (GTs) provide information about the expected performance of cattle. Cattle producer survey results indicated that 56% of producers were interested in using GTs for marketing cattle and 74% would use GTs to select replacement heifers. If interested in using GTs to market cattle, on average, producers indicated they were willing to pay $21/head and test 55% of their animals. For replacement heifers, on average, they were willing to pay $23/head and test 77% of their heifers. A conditional mixed process regression framework found producer characteristics and risk preferences were associated with the decisions involved in using GTs.
Research and scholarly methods: Thematic analysis
Authors: Cernasev Alina , Axon David R. 
Published: May 2023

AbstractThematic analysis (TA) is a commonly used approach in qualitative research. TA has been widely applied in various disciplines such as psychology, sociology, and anthropology, and is now increasingly employed in pharmacy and healthcare research. However, pharmacy researchers may not be familiar with TA. The objectives of this manuscript are to succinctly describe the process for TA explained through the six steps of Braun and Clark as it applies to pharmacy or healthcare‐related research, discuss how rigor is achieved in qualitative studies, provide an overview of the qualitative software that is available to assist with TA, and offer comparisons to quantitative analysis where appropriate to demonstrate the contrast with qualitative techniques such as TA.
Label‐free histological analysis of retrieved thrombi in acute ischemic stroke using optical diffraction tomography and deep learning
Authors: Chung Yoonjae , Kim Geon , Moon Ah‐Rim , Ryu DongHun , Hugonnet Herve , Lee Mahn Jae , Shin DongSeong , Lee Seung‐Jae , Lee Eek‐Sung , Park YongKeun 
Published: May 2023

AbstractFor patients with acute ischemic stroke, histological quantification of thrombus composition provides evidence for determining appropriate treatment. However, the traditional manual segmentation of stained thrombi is laborious and inconsistent. In this study, we propose a label‐free method that combines optical diffraction tomography (ODT) and deep learning (DL) to automate the histological quantification process. The DL model classifies ODT image patches with 95% accuracy, and the collective prediction generates a whole‐slide map of red blood cells and fibrin. The resulting whole‐slide composition displays an average error of 1.1% and does not experience staining variability, facilitating faster analysis with reduced labor. The present approach will enable rapid and quantitative evaluation of blood clot composition, expediting the preclinical research and diagnosis of cardiovascular diseases.