bluuebunny/crossref_metadata_2025_split huggingface dataset:
PERTUMBUHAN KULTUR MERISTEM MAHKOTA NANAS (Ananas comosus (L.) Merr) PADA MEDIA MURASHIGE SKOOG (MS) DENGAN PENAMBAHAN EKSTRAK JAGUNG (Zea mays (L.) DAN Naphthalene Acetic Acid (NAA)
Authors: Desy Desy , Mukarlina  , Elvi Rusmiyanto  
Published: July 2023

Nanas (Ananas comosus (L.) Merr.) merupakan salah satu jenis buah-buahan holtikultura yang berpotensi untuk dibudidayakan dan memiliki nilai ekonomi baik di Kalimantan Barat. Perbanyakan tanaman nanas dapat dilakukan secara in vitro melalui kultur meristem mahkota nanas dengan menumbuhkan eksplan pada media Murashige Skoog dengan penambahan ekstrak biji jagung dan Naphthalene Acetic Acid (NAA). Tujuan penelitian adalah untuk mengetahui pengaruh ekstrak biji jagung dan NAA terhadap pertumbuhan kultur meristem mahkota nanas. Penelitian dilaksanakan bulan September 2019 hingga April 2020 di Laboratorium Kultur Jaringan Jurusan Biologi FMIPA Universitas Tanjungpura. Penelitian ini menggunakan Rancangan Acak Lengkap (RAL) dengan dua faktor. Faktor pertama ekstrak biji jagung konsentrasi 0% (J1), 10% (J2), dan 15% (J3) ; faktor kedua NAA konsentrasi 0M (N1), 5x10-8 M (N2), dan 10-7 M (N3) . Masing-masing perlakuan diulang 3 kali sehingga diperoleh 27 unit percobaan. Hasil penelitian menunjukkan bahwa kombinasi NAA dan ekstrak biji jagung berpengaruh nyata terhadap waktu muncul tunas (hari), jumlah tunas (tunas), jumlah daun (helai) dan tinggi planlet (cm). Perlakuan 10-7 M NAA+15% ekstrak biji jagung (N1J2) menghasilkan pertumbuhan terbaik dengan waktu muncul tunas 8,6 hari dan jumlah tunas 7 buah tunas.
JENIS PTERIDOPHYTA YANG TERDAPAT DI KAWASAN WISATA BRAYEUN KECAMATAN LEUPUNG KABUPATEN ACEH BESAR
Authors: Fauziah Asyifa , Hasanuddin Hasanuddin , Andayani Dewi , Nurmaliah Cut , Wardiah Wardiah 
Published: July 2023

Penelitian ini bertujuan untuk mengetahui jenis dan jumlah pteridophyta, jenis pteridophyta epifit dan teresterial serta jenis pteridophyta dimorfisme yang terdapat di kawasan Wisata Brayeun. Penelitian dilaksanakan pada bulan Juni hingga Juli tahun 2021. Penelitian ini menggunakan metode survei eksploratif dengan teknik purposive sampling. Data dianalisis secara deskriptif. Hasil penelitian: (1) Terdapat 23 Jenis pteridophyta yang tergolong kedalam 13 familia (2) Pada Kawasan Wisata Brayeun ditemukan 15 jenis pteridophyta yang hidup secara teresterial dan 6 jenis pteridophyta yang hidup secara epifit yaitu Drymoglossum piloselloides, Drynaria quercifolia, Microsorium fortunei, Pyrrosia lanceolata, Aspelium nidus dan Davalia trichomanoides (3) ditemukan 4 jenis pteridophyta dimorfisme yaitu Stenochlaena palustris, Pteris ensiformis, Drymoglossum piloselloides dan Drynaria quercifolia.
The PROFIL FITOKIMIA DAN AKTIVITAS ANTIOKSIDAN EKSTRAK AIR DAUN Tegetes erecta L
Authors: Fitrah Asma  Ulhusna  
Published: July 2023

Tanaman Tegetes erecta L merupakan tumbuhan herba yang tumbuh di daerah tropis. Tujuan penelitian adalah untuk menganalisis senyawa bioaktif dan aktivitas antioksidan edari kstrak air daun T. erecta. Uji fitokimia dilakukan secara kualitatif. Aktivitas antioksidan dianalisis dengan metode DPPH. Hasil dari penelitian ini menunjukkan bahwa kandungan bioaktif pada ekstrak air daun T. erecta adalah alkaloid, saponin, tannin, flavonoid, steroid, dan polifenol tetapi negatif untuk kuinon dan triterpenoid. Ekstrak daun T. erecta dengan pelarut air memiliki aktivitas antioksidan sebesar 17.08 ppm. Kesimpulan dari penelitian adalah adalah ekstrak air daun T. erecta memiliki senyawa bioakatif berupa alkaloid, saponin, tannin, flavonoid, steroid, dan polifenol serta ekstrak air daun T. erecta memiliki aktivitas antioksidan yang sangat kuat yaitu 17.08 ppm.
Kata Kunci: antioksidan, ekstrak, fitokimia, Tagetes erecta L.
Pengembangan LKPD Berbasis Guided Inquiry pada Materi Sistem Pernapasan Manusia
Authors: Damanik Nurul Nazmi , Rasyidah  , Rambe Riris Nurkholidah 
Published: July 2023

Dilaksanakannya suatu pembelajaran Guided Inquiry yaitu menuntun peserta didik untuk memperoleh pengetahuan melalui kegiatan pembelajaran mandiri, maka perlu adanya LKPD yang dapat mengarahkan peserta didik untuk mencari dan berusaha mendapatkan jawaban dari permasalahan yang ada dalam pembelajaran. Metode penelitian yang digunakan adalah Research and Development (R&D). Model yang digunakan dalam mengembangkan produk pembelajaran adalah model ADDIE. Kepraktisan LKPD berbasis guided inquiry pada materi sistem pernapasan manusia mendapatkan tanggapan dari peserta didik dari kelas XI IPA-1 yaitu 81,79% dengan keterangan “Sangat Praktis”, untuk kelas XI IPA-2 mendapatkan tanggapan sebesar 87,30% dengan keterangan “Sangat Praktis”, selanjutnya validasi praktisi lapangan sebesar 94.8% yaitu dengan keterangan “Sangat Praktis” Terdapat perbedaan jumlah rata-rata skor gain ternormalisasi pada kelas XI IPA-1 dan IPA -2, rata-rata skor gain ternormalisasi kelas IPA-2 lebih tinggi dibandingkan dengan kelas XI IPA-1 dengan selisih skor 0,03, namun, diantara keduanya berada pada kategori “Efektif”.
Status of screening and preventive efforts against diabetic kidney disease between 2013 and 2018: analysis using an administrative database from Kyoto-city, Japan
Authors: Tateyama Yukiko , Shimamoto Tomonari , Uematsu Manako K. , Taniguchi Shotaro , Nishioka Norihiro , Yamamoto Keiichi , Okada Hiroshi , Takahashi Yoshimitsu , Nakayama Takeo , Iwami Taku 
Published: July 2023

BackgroundJapan has the second highest prevalence of dialysis use in the world. Approximately 40% of patients who begin dialysis have diabetic kidney disease (DKD). Local governments and medical facilities are required to provide preventive measures against worsening diabetes mellitus (DM). However, the percentage of patients with DM who receive such screening or interventions for DKD is unclear. This study aimed to reveal to what extent screening for DKD and preventive measures against worsening DKD are performed in patients with DM, using an administrative database in a municipality.MethodsThis was a cross-sectional study that used the Kyoto-city’s administrative medical and long-term care database. Patients with a diagnosis of DM and receiving antidiabetic medication between 2013 and 2018 were defined as patients with DM and included. Patients with DKD were defined as those diagnosed with diabetic nephropathy or those with chronic kidney disease. We described the characteristics of patients with DM, diabetic complications, and extent of DKD screenings and preventive efforts against worsening of DM by fiscal year.ResultsAcross fiscal years, 25.8% to 27.5% of patient with DM had DKD. More than 3% of patients were on dialysis due DM in each fiscal year; approximately 15% started receiving dialysis that year. The percentage of patients who were regularly prescribed antidiabetic medication and received glycosylated hemoglobin testing ranged from 64.0% to 67.2% and from 30.6% to 36.5%, respectively. Urine microalbuminuria testing at least once a year occurred in 9.3% to 10.0%. The percentage of patients who received nutritional guidance ranged from 19.0% to 21.0%. Approximately 1% of patients received guidance for preventing DM from progressing to a disease that requires dialysis each fiscal year.ConclusionThis study from Japan, where a super-aging society has developed, using an administrative database in a municipality covering most of the elderly population clearly demonstrated an evidence-practice gap in efforts to prevent worsening of DKD. Strengthening cooperation between government and medical facilities and support for providing preventive measures against DKD are urgently needed.
Combining network pharmacology, molecular docking, molecular dynamics simulation, and experimental verification to examine the efficacy and immunoregulation mechanism of FHB granules on vitiligo
Authors: Li Xiaolong , Miao Fengze , Xin Rujuan , Tai Zongguang , Pan Huijun , Huang Hao , Yu Junxia , Chen Zhongjian , Zhu Quangang 
Published: July 2023

BackgroundFufang Honghua Buji (FHB) granules, have proven efficacy against vitiligo in long-term clinical practice. However, its major active chemical components and molecular mechanisms of action remain unknown. The purpose of this study was to confirm the molecular mechanism of FHB’s therapeutic effect on vitiligo utilizing network pharmacology, molecular docking, and molecular dynamics simulation prediction, as well as experimental verification.MethodsTraditional Chinese Medicine Systems Pharmacology (TCMSP) and HERB databases were used to obtain the chemical composition and action targets of FHB. Online Mendelian Inheritance in Man (OMIM), DrugBank, DisGeNET, GeneCards, and Therapeutic Target Database (TTD) databases were applied to screen for vitiligo-related targets. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses were performed through the Matascape database. Molecular docking and dynamics simulation methods were for the analysis of the binding sites and binding energies between the FHB’s active components and the targets. Finally, a vitiligo mouse model was created, and the therapeutic effect and molecular mechanism of action of FHB were validated using enzyme linked immunosorbent assay (ELISA), western blot (WB), and quantitative reverse transcription-polymerase chain reaction (qRT-PCR). Additionally, hematoxylin-eosin staining (HE) and blood biochemical assays were conducted to assess the biosafety of FHB.ResultThe screening of chemical composition and targets suggested that 94 genetic targets of FHB were associated with vitiligo. The bioinformatics analysis suggested that luteolin, quercetin, and wogonin may be major active components, and nuclear factor-kappa B p65 subunit (RELA), signal transducer, and activator of transcription (STAT) 3 and RAC-alpha serine/threonine-protein kinase (AKT) 1 may be potential targets of FHB-vitiligo therapy. Molecular docking and dynamics simulation further demonstrated that luteolin, quercetin, and wogonin all bound best to STAT3. Through experimental verification, FHB has been demonstrated to alleviate the pathogenic characteristics of vitiligo mice, suppress the JAK-STAT signaling pathway, reduce inflammation, and increase melanogenesis. The in vivo safety evaluation experiments also demonstrated the non-toxicity of FHB.ConclusionsFHB exerts anti-inflammatory and melanogenesis-promoting effects via the effect of multi-component on multi-target, among which the JAK-STAT pathway is a validated FHB-vitiligo target, providing new ideas and clues for the development of vitiligo therapy.
Phorbol-12-myristate-13-acetate is a potent enhancer of B cells with a granzyme B+ regulatory phenotype
Authors: Veh Johanna , Mangold Charlotte , Felsen Anja , Ludwig Carolin , Gerstner Lisa , Reinhardt Peter , Schrezenmeier Hubert , Fabricius Dorit , Jahrsdörfer Bernd 
Published: July 2023

IntroductionThe infusion of ex-vivo-generated regulatory B cells may represent a promising novel therapeutic approach for a variety of autoimmune and hyperinflammatory conditions including graft-versus-host disease.MethodsPreviously, we developed a protocol for the generation of a novel population of regulatory B cells, which are characterized by secretion of enzymatically active granzyme B (GraB cells). This protocol uses recombinant interleukin 21 (IL-21) and goat-derived F(ab)’2 fragments against the human B cell receptor (anti-BCR). Generally, the use of xenogeneic material for the manufacturing of advanced therapy medicinal products should be avoided to prevent adverse immune reactions as well as potential transmission of so far unknown diseases.ResultsIn the present work we demonstrated that phorbol-12-myristate-13-acetate (PMA/TPA), a phorbol ester with a particular analogy to the second messenger diacylglycerol (DAG), is a potent enhancer of IL-21-induced differentiation of pre-activated B cells into GraB cells. The percentage of GraB cells after stimulation of pre-activated B cells with IL-21 and PMA/TPA was not significantly lower compared to stimulation with IL-21 and anti-BCR.DiscussionGiven that PMA/TPA has already undergone encouraging clinical testing in patients with certain haematological diseases, our results suggest that PMA/TPA may be a safe and feasible alternative for ex-vivo manufacturing of GraB cells.
A novel stratification framework based on anoikis-related genes for predicting the prognosis in patients with osteosarcoma
Authors: Zhang Xiaoyan , Wen Zhenxing , Wang Qi , Ren Lijuan , Zhao Shengli 
Published: July 2023

BackgroundAnoikis resistance is a prerequisite for the successful development of osteosarcoma (OS) metastases, whether the expression of anoikis-related genes (ARGs) correlates with OS prognosis remains unclear. This study aimed to investigate the feasibility of using ARGs as prognostic tools for the risk stratification of OS.MethodsThe Cancer Genome Atlas (TCGA) and Gene Expression Omnibus (GEO) databases provided transcriptome information relevant to OS. The GeneCards database was used to identify ARGs. Differentially expressed ARGs (DEARGs) were identified by overlapping ARGs with common differentially expressed genes (DEGs) between OS and normal samples from the GSE16088, GSE19276, and GSE99671 datasets. Anoikis-related clusters of patients were obtained by consistent clustering, and gene set variation analysis (GSVA) of the different clusters was completed. Next, a risk model was created using Cox regression analyses. Risk scores and clinical features were assessed for independent prognostic values, and a nomogram model was constructed. Subsequently, a functional enrichment analysis of the high- and low-risk groups was performed. In addition, the immunological characteristics of OS samples were compared between the high- and low-risk groups, and their sensitivity to therapeutic agents was explored.ResultsSeven DEARGs between OS and normal samples were obtained by intersecting 501 ARGs with 68 common DEGs. BNIP3 and CXCL12 were significantly differentially expressed between both clusters (P&lt;0.05) and were identified as prognosis-related genes. The risk model showed that the risk score and tumor metastasis were independent prognostic factors of patients with OS. A nomogram combining risk score and tumor metastasis effectively predicted the prognosis. In addition, patients in the high-risk group had low immune scores and high tumor purity. The levels of immune cell infiltration, expression of human leukocyte antigen (HLA) genes, immune response gene sets, and immune checkpoints were lower in the high-risk group than those in the low-risk group. The low-risk group was sensitive to the immune checkpoint PD-1 inhibitor, and the high-risk group exhibited lower inhibitory concentration values by 50% for 24 drugs, including AG.014699, AMG.706, and AZD6482.ConclusionThe prognostic stratification framework of patients with OS based on ARGs, such as BNIP3 and CXCL12, may lead to more efficient clinical management.
A contrast-enhanced ultrasound-based nomogram for the prediction of therapeutic efficiency of anti-PD-1 plus anti-VEGF agents in advanced hepatocellular carcinoma patients
Authors: Sun Chao , Wang Qian , Hou Lu , Zhang Rui , Chen Yu , Niu Lijuan 
Published: July 2023

BackgroundThere is no study focusing on noninvasive predictors for the efficacy of sintilimab (anti-PD-1) plus IBI305 (a bevacizumab biosimilar) treatment in advanced hepatocellular carcinoma (HCC).MethodA total of 33 patients with advanced HCC were prospectively enrolled and received sintilimab plus IBI305 treatment from November 2018 to October 2019. Baseline characteristics including clinical data, laboratory data, and tumor features based on pretreatment CT/MR were collected. Meanwhile, pretreatment contrast-enhanced ultrasound (CEUS) for target tumor was performed and quantitative parameters were derived from time–intensity curves (TICs). A nomogram was developed based on the variables identified by the univariable and multivariable logistic regression analysis. The discrimination, calibration, and clinical utility of the nomogram were evaluated.ResultsTumor embolus and grad ratio were significant variables related to the efficacy of sintilimab plus IBI305 strategy. The nomogram based on these two variables achieved an excellent predictive performance with an area under curve (AUC) of 0.909 (95% CI, 0.813–1). A bootstrapping for 500 repetitions was performed to validate this model and the AUC of the bootstrap model was 0.91 (95% CI, 0.8–0.98). The calibration curve and decision curve analysis (DCA) showed that the nomogram had a good consistency and clinical utility.ConclusionsThis study has established and validated a nomogram by incorporating the quantitative parameters of pretreatment CEUS and baseline clinical characteristics to predict the anti-PD-1 plus anti-VEGF treatment efficacy in advanced HCC patients.
The relationship between parent–child relationship and peer victimization: a multiple mediation model through peer relationship and depression
Authors: Zhou Pingyan , Dong Jinqi , Liu Jian , Wen Hongbo , Wang Zhe 
Published: July 2023

IntroductionPeer victimization is a highly prevalent worldwide issue with cross-cultural characteristics. Parent–child relationship and peer victimization is known to be interrelated, but how they influence each other remains unclear. This study explored the mechanisms of peer victimization related to parent–child relationship.MethodsA total of 58,756 fourth grade students aged 10–12 years (10.83 ± 0.83, 54.4% males) from China completed four questionnaires. A multiple mediator model was tested, in which the two variables influenced each other through the mediating factors of peer relationship and depression.ResultsPeer victimization was indirectly negatively impacted by parent–child relationship through two chain mediating factors of peer relationship and depression: (1) the mediational path through peer relationship with an effect size of 44.66%; (2) the mediational path through depression with an effect size of 21.64%; and (3) the mediational path through peer relationship and depression with an effect size of 18.08%. The total mediational effect size was 84.11%.ConclusionThe effect size through peer relationship is the strongest among the three mediation paths, suggesting that peer relationship is the key determinant in breaking the link between parent–child relationship and victimization. Poor parent–child and peer relationships may be risk factors eliciting peer victimization. Compared to internalizing behaviors (e.g., depression), low-quality interpersonal relationships maybe the root cause of the formation and maintenance of victimization. Thus, intervention programs against bullying should pay more attention on children’s contextual factors, especially their relationships with their families and peers, among children at an early age.
School-based social skills group training (SKOLKONTAKT™): a pilot randomized controlled trial
Authors: Fridell Anna , Coco Christina , Borg Anna , Bölte Sven 
Published: July 2023

PurposeDifferences in socio-communicative behaviors contribute to social challenges for autistic learners at school and, in turn, are associated with increased risks of educational underachievement, social exclusion, and mental health issues. Given that intervention delivery in natural contexts may enhance skills generalization, build support capacities in society, and have practical advantages for youth and families, SKOLKONTAKT™ has been adapted from the clinically based social skills group training KONTAKT™ for mainstream educational settings to mitigate these risks.MethodsA pilot, randomized controlled trial with active controls was conducted in a mainstream Swedish high school. Autistic learners and students with social skills challenges (N = 33; MAGE = 17.5) were randomized to SKOLKONTAKT™ (n = 17) or active control (n = 16). Efficacy was measured at post and follow-up (3 months) on social skills [Social Skills Group Assessment Questionnaire (SSGQ); primary outcome] by parent-, self-, and (masked) teacher-report as well as self-reported life quality and social goal attainment.ResultsDespite COVID-19 challenges, 70.6% (n = 12) completed SKOLKONTAKT™, and 87.5% (n = 14) completed control groups. SKOLKONTAKT™ improved on a series of items on SSGQ as well as subjective life quality beyond controls. A larger proportion of social goals were attained, and side-effects were of little impact and proportionally fewer in SKOLKONTAKT™.ConclusionSKOLKONTAKT™ is a safe, feasible, and promising intervention option for autistic learners in mainstream educational settings. A larger-scale study is desirable to confirm the effects identified in this pilot study.
The psychological symptoms and behavioral problems of children with mothers working as medical staff in the crisis of Covid-19 outbreak in Hamadan, Iran
Authors: Raeisi Roya , Gholamzad Shakiba , Dehkordi Mansoureh Kiani , Kheirabadi Mehri Rezaei , Ddehkordi Ali Hhasanpour , Sobhani Mohammad Mahdi , Movahedi Mahsa 
Published: July 2023

Background and objectivesThe purpose of this study was to investigate the psychological symptoms and behavioral problems of children with mothers working as medical staff in the crisis of Covid-19 disease in Hamadan.MethodsThis descriptive causal-comparative study was conducted on all mothers with children aged 6 to 12  years in Hamadan from September 2 to November 29, 2020. In this study, eligible individuals were selected using random sampling and were assigned to two groups of mothers working as the medical staff and the control group. The research instruments included the Child Behavior Checklist (Achenbach) and the Child Symptom Inventory-4 (CSI-4).ResultsThe results showed that the mean scores of psychological and behavioral symptoms of children in terms of group membership (group of mothers working in the medical staff and control group) had a significant difference. There was a significant difference between the mean scores of depression and aggression in children of the staff group and the control group meaning that for depression and aggression scores of children of the staff group are higher than children of the control group (p &lt; 0.05). There was no significant difference between the mean anxiety scores and there was almost a significant difference between the attention scores of the staff group and the control group (p &lt; 0.05).ConclusionChildren whose mothers worked as medical staff during Covid-19 show more depression, attention, and aggression problems than children whose mothers do not work as medical staff.