bluuebunny/crossref_metadata_2025_split huggingface dataset:
Immunohistochemical staining seems mandatory for individualizing and shortening follow‐up in unilateral primary aldosteronism
Authors: Gunnarsdottir Hrafnhildur , Agnarsson Bjarni A. , Jonasdottir Sigurros , Gudmundsson Jon , Birgisson Gudjon , Sigurjonsdottir Helga A. 
Published: August 2023

AbstractObjectiveThe clinical significance of immunohistochemistry (IHC) for unilateral primary aldosteronism (PA) has been unclear. Individualized follow‐up of PA patients could be in sight. Long‐term outcomes of patients, classified based on IHC, need further investigation. We aimed to assess long‐term clinical outcomes for unilateral PA, classifying patients based on IHC.DesignA nationwide observational study, with up to 16 years follow‐up, executed in 2007–2016 at Landspitali University Hospital, tertiary referral center. Patients were diagnosed and treated in line with the current guidelines. Haematoxylin and eosin (H&E) tissue slides were stained using CYP11B1 and ‐B2 antibodies. All cases were re‐evaluated and classified according to the HISTALDO consensus. Outcomes were assessed using the PASO criteria.PatientsAll unilateral PA patients diagnosed in 2007‐2016 in Iceland, 26 patients aged 28–73 years, who underwent adrenalectomy, were included.MeasurementsAldosterone, renin, and cortisol values, use and dosage of antihypertensives, potassium supplementation, blood pressure and serum potassium pre‐intervention and throughout follow‐up, and histopathology results post‐adrenalectomy.ResultsFollowing IHC, an aldosterone‐producing nodule was seen in 12 adrenals, an aldosterone‐producing adenoma in 10 and multiple aldosterone‐producing micronodules in four. IHC altered histopathology from previous H&E diagnosis in 23% (6/26) of the patients. In total, 81% (21/26) of the patients had partial clinical success. Eight percent (2/26) of the patients needed potassium supplementation during follow‐up. In the classical group, the AVS results were more determinative with significantly higher lateralization index (median 10.1 vs. 5.3, p = .04) and more contralateral suppression (median nondominant ratio 0.4 vs. 1.0, p = .03). One out of five patients with complete clinical success at 12 months post‐op had severe relapse later, the other four were normotensive without antihypertensives for up to 10 years.ConclusionsWe found IHC mandatory for accurate histopathologic diagnosis of PA. Our results support the importance of contralateral suppression when interpreting AVS results. Also, the study highlights the complicated assessment of clinical outcome and importance of aldosterone and renin measurements during follow‐up.
Clinical evaluation and quantitative occlusal change analysis of posterior implant‐supported all‐ceramic crowns: A 3‐year randomized controlled clinical trial
Authors: Zhang Yifan , Wei Donghao , Tian Jiehua , Zhao Yijiao , Lin Ye , Di Ping 
Published: August 2023

AbstractObjectivesTo compare the survival and complication rates of posterior screw‐retained monolithic lithium disilicate (LS2)/veneered zirconia (ZrO2) single implant crowns (SICs), as well as analyze the occlusal changes observed during a 3‐year follow‐up period.Materials and MethodsThirty‐three patients were included and randomly divided into two groups. The test group consisted of 17 patients who received monolithic‐LS2–SIC, while the control group consisted of 16 patients who received veneered‐ZrO2‐SIC. Implant/prosthesis survival rates, technical complications, peri‐implant soft tissue conditions, and quantitative occlusal changes of SIC (obtained by the intra‐oral scanner and analyzed in reverse software Geomagic Control 2015) were assessed at 1‐ and 3‐year follow‐ups. Bone loss and Functional Implant Prosthodontic Score (FIPS) were evaluated at a 3‐year follow‐up.ResultsAfter a 3‐year follow‐up period, one patient dropped out of the follow‐up. No implant loss was observed. One crown was fractured, resulting in prosthesis survival rates of 93.75% for the monolithic group and 100% for the veneered group. A technical complication rate of 25% (4/16) was observed in the veneered group (p = .333). No significant differences in the marginal bone loss were observed at the 3‐year follow‐up (0.00 (−0.22, 0.17) mm versus 0.00 (−0.12, 0.12) mm, p = .956). The total FIPS scores for the test group were 9.0 (9.0, 9.0), while the control group received scores of 9.0 (8.0, 10.0) (p = .953). The changes in mean occlusal clearance were 0.022 ± 0.083 mm for the test and 0.034 ± 0.077 mm for the control group (at 3 years, p = .497). The changes in occlusal contact area were 1.075 ± 2.575 mm2 for the test and 1.676 ± 2.551 mm2 for the control group (at 3 years, p = .873).ConclusionAfter a 3‐year follow‐up, screw‐retained monolithic LS2 and veneered ZrO2 SIC demonstrated similar survival rates. The occlusal performance of implant prostheses needs to be closely examined during follow‐up, and appropriate occlusal adjustments need to be considered.
Featured Cover
Authors: Yao Yunqian , Wei Ling , Chen Zhenhua , Li Hao , Qi Jiao , Wu Qingfeng , Zhou Xingtao , Lu Yi , Zhu Xiangjia 
Published: August 2023

The cover image is based on the Original Article Single‐cell RNA sequencing: Inhibited Notch2 signalling underlying the increased lens fibre cells differentiation in high myopia by Yunqian Yao et al., https://doi.org/10.1111/cpr.13412.
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Comparison of glutaminase and cancer antigen 125 for distinguishing benign and malignant ovarian tumors
Authors: Winarno Gatot Nyarumenteng Adhipurnawan , Effendi Jusuf Sulaeman , Harsono Ali Budi , Salima Siti , Darwizar Bagja Dumas , Sasotya R. M. Sonny , Rachmawati Anita , Mulyantari Ayu Insafi , Trianasari Nurvita , Handono Budi 
Published: August 2023

AbstractIncreasing demand for glutaminase (GLS) due to high rates of glutamine metabolism is considered one of the hallmarks of malignancy. In parallel, cancer antigen 125 (CA‐125) is a commonly used ovarian tumor marker. This study aimed to compare the roles of GLS and CA‐125 in distinguishing between benign and malignant ovarian tumors. The research was conducted as a comparative study, enrolling 156 patients with ovarian tumors. Preoperative serum CA‐125 and GLS levels were analyzed to evaluate their effectiveness in distinguishing between benign and malignant ovarian tumors. The results revealed that the mean levels of CA‐125 and GLS were significantly higher in malignant ovarian tumors compared with benign ones (389.54 ± 494.320 vs. 193.15 ± 529.932 (U/mL) and 17.37 ± 12.156 vs. 7.48 ± 4.095 (μg/mL), respectively). The CA‐125 and GLS cutoff points of 108.2 U/mL and 18.32 μg/mL, respectively, were associated with malignant ovarian tumors. Multivariate analyses showed that GLS had higher predictive capabilities compared with CA‐125 (odds ratio 9.4 vs. 2.1). The accuracy of using GLS combined with CA‐125 was higher than using CA‐125 alone (73.1% vs. 68.8%). In conclusion, higher levels of CA‐125 and GLS are associated with malignant ovarian tumors. GLS outperforms CA‐125 in distinguishing between benign and malignant ovarian tumors. The combination of GLS and CA‐125 demonstrated improved accuracy for distinguishing benign and malignant ovarian tumors when compared with using CA‐125 alone.
Does the advent of depot therapy represent a step change in our understanding of opioid use disorder and its treatment?
Authors: Roberts Emmert , Humphreys Keith 
Published: August 2023

AbstractAfter years of minimal innovation in pharmacotherapeutics, impressive outcomes in the treatment of opioid use disorder are being obtained from a new way of delivering an old medication; long‐acting injectable formulations of buprenorphine appear to produce compelling reductions in relapse to illicit opioid use not only during use but also following depot discontinuation. This commentary discusses potential mechanisms behind this observation, asks if the removal of the need for daily oral opioid agonist dosing furthers our understanding of addiction treatment and whether we should therefore consider expanding access to depot formulations.
Replication and extension of the military family stress model: The after deployment adaptive parenting tools ADAPT4U study
Authors: Cheng Cheuk H. , Ali‐Saleh Darawshy Neveen , Lee Susanne , Brigman Hayley , DeGarmo Dave , Gewirtz Abigail 
Published: August 2023

AbstractThe military family stress (MFS) model conceptualizes that wartime deployments and post‐traumatic stress disorder (PTSD) symptoms are associated with couple, parenting, and child adjustment difficulties. The aim of this study was to replicate and extend the military family stress model by examining the associations among deployment length, PTSD symptoms, marital functioning, parenting practices, and child adjustment in a replication sample of both National Guard and Reserve (NG/R) as well as active‐duty service member families. The MFS model is extended to test whether these relationships vary between mothers and fathers. The sample included 208 families enrolled in a randomized controlled trial of a parenting program for military families (94.4% of fathers and 21.6% of mothers were deployed). Replicating the MFS model, we specified parenting, marital quality, and child adjustment as latent variables and conducted multi‐group structural equation models. Parenting practices were positively associated with marital quality and child adjustment. PTSD symptoms were negatively associated with marital quality. The indirect effect from PTSD symptoms to parenting practices through marital quality was marginally significant. The indirect effect from marital quality to child adjustment through parenting practices was significant. There were no significant gender differences between the two structural models. This study provides empirical support for the MFS model. Results demonstrate that deployment‐related stressors are significantly associated with parent and family functioning. Parenting programs for military families might effectively target similar risk processes among both mothers and fathers.
‘Good, honest food’: older adults' and healthcare professionals' perspectives of dietary influences and food preferences in older age in Ireland
Authors: Mahony Lauren O. , Shea Emma O. , O'Connor Eibhlís M. , Tierney Audrey , Harkin Mary , Harrington Janas , Kennelly Sharon , Arendt Elke , O'Toole Paul W. , Timmons Suzanne 
Published: August 2023

AbstractBackgroundThis study aimed to explore older adults' and healthcare professionals' (HCPs) perceptions of dietary influences and food preferences in older age.MethodsThe research design was phenomenological qualitative description. Semistructured one‐to‐one interviews and focus groups were held separately with community‐dwelling older adults and HCPs involved in care of the older person in Ireland. Data were analysed using inductive thematic analysis.ResultsA total of 47 adults aged 55+ years were recruited (50% male; 49% aged 60–69 years; 28% aged above 70 years), and 26 HCPs were involved, comprising dietitians (n = 8); geriatricians (n = 6); clinical therapists (n = 4); and nurses, pharmacists, catering managers and meal delivery service coordinators (n = 2 each). There are strong desires for ‘good, honest food’ within the diet for an older person; however, gaps in current nutrition priorities, dietary guidance and health promotion were perceived. There were differences in the perspectives held by HCPs and adults aged 55+ years, as some HCPs centred their discussion around nutrition for preventing sarcopenia, frailty or cognitive decline, whereas many adults aged 55+ years desired foods which promote cardiometabolic health and reflect wider personal health and environmental values. Other themes included the impact of health and lifestyle changes accompanying ageing on dietary priorities, the importance of personal and psychosocial values in determining food choice and the impact of the external food environment on accessibility and shopping experiences.ConclusionsInfluences on dietary choice for the older person are multifactorial, driven by a range of health, psychological, sociocultural and environmental perspectives. Future nutrition priorities for older adults should encourage health‐promoting approaches and not just disease‐mitigating efforts.
A case study of the impact of a dietitian in the multi‐disciplinary team within primary care: a service evaluation
Authors: Hickson Mary , Child Jenny , Collinson Avril 
Published: August 2023

AbstractBackgroundExpanding the primary care workforce to alleviate general practitioner (GP) workload, improve access and improve quality of care is a current UK strategy. Evidence suggests dietitians can improve patient outcomes and make cost savings. The present study aimed to evaluate a dietitian working as an expert generalist and first contact practitioner (FCP) in a general practice multi‐disciplinary team (MDT) to provide appropriate care to patients and reduce GP workload.MethodsA dietitian was employed for 6 months at 0.6 full‐time equivalents in a group of general practices in Devon, UK. Data were collected on the referral source, patient satisfaction, health outcomes and changes in prescribing data for all patients seen by the dietitian. Focus groups and interviews provided data to understand the experience of introducing a dietitian into the team.ResultsThis model of service delivery showed the dietitian acting as an expert generalist, a FCP and able to educate the MDT. A range of professionals within the MDT referred patients with a wide range of diagnoses (both paediatric and adults) and the dietitian acted as a FCP for 29% of patients. Saving were made for the optimisation of medicine management.ConclusionsThe dietitian can improve patient‐centred care for several patient groups; enhance learning for staff around nutrition and dietary issues; and contribute to more efficient working and cost savings around prescription of nutritional products. This was an evaluation of one service and further research is needed to understand the value dietitians can contribute and the factors supporting effective and efficient working in this context.
Klebsiella pneumoniae infection in cynomolgus monkeys at primate research center facility in Indonesia
Authors: Saputro Suryo , Saepuloh Uus , Darusman Huda Shalahudin , Putriyani Wahyu , Permanawati  , Ayuningsih Ellis Dwi , Prabandari Silvia Arin , Setyawati Dyah , Pamungkas Joko 
Published: August 2023

AbstractBackgroundKlebsiella pneumoniae infection in nonhuman primates has been widely reported and causes significant morbidity and mortality. Animal deaths occur routinely at the Primate Research Center of IPB University. The results of necropsy and culture suggested a K. pneumoniae infection.MethodsA mass health assessment of Cynomolgus monkeys (n = 429) was carried out by physical examination and molecular targeting K. pneumoniae (n = 96), family of Coronaviridae (n = 148) and Paramyxoviridae (n = 148).ResultsA total of 49.18% of the animals had clinical symptoms of respiratory disorders, abscesses, trauma, and others. PCR results indicated that 28.57% were positive for K. pneumoniae with 35.71% mortality, while all samples were negative for both virus families.ConclusionsThere have been outbreaks caused by K. pneumoniae and/or K. pneumoniae subsp. pneumoniae. This disease is chronic, infects all of the buildings, and no tendency for disease transmission according to gender and age class.
Cooperative and competitive regulation of the astrocytic transcriptome by neurons and endothelial cells: Impact on astrocyte maturation
Authors: Martinez‐Lozada Zila , Farmer W. Todd , Schober Alexandra L. , Krizman Elizabeth , Robinson Michael B. , Murai Keith K. 
Published: August 2023

AbstractAstrocytes have essential roles in central nervous system (CNS) health and disease. During development, immature astrocytes show complex interactions with neurons, endothelial cells, and other glial cell types. Our work and that of others have shown that these interactions are important for astrocytic maturation. However, whether and how these cells work together to control this process remains poorly understood. Here, we test the hypothesis that cooperative interactions of astrocytes with neurons and endothelial cells promote astrocytic maturation. Astrocytes were cultured alone, with neurons, endothelial cells, or a combination of both. This was followed by astrocyte sorting, RNA sequencing, and bioinformatic analysis to detect transcriptional changes. Across culture configurations, 7302 genes were differentially expressed by 4 or more fold and organized into 8 groups that demonstrate cooperative and antagonist effects of neurons and endothelia on astrocytes. We also discovered that neurons and endothelial cells caused splicing of 200 and 781 mRNAs, respectively. Changes in gene expression were validated using quantitative PCR, western blot (WB), and immunofluorescence analysis. We found that the transcriptomic data from the three‐culture configurations correlated with protein expression of three representative targets (FAM107A, GAT3, and GLT1) in vivo. Alternative splicing results also correlated with cortical tissue isoform representation of a target (Fibronectin 1) at different developmental stages. By comparing our results to published transcriptomes of immature and mature astrocytes, we found that neurons or endothelia shift the astrocytic transcriptome toward a mature state and that the presence of both cell types has a greater effect on maturation than either cell alone. These results increase our understanding of cellular interactions/pathways that contribute to astrocytic maturation. They also provide insight into how alterations to neurons and/or endothelial cells may alter astrocytes with implications for astrocytic changes in CNS disorders and diseases.image
Microbiological profile of peri‐implantitis: Analyses of peri‐implant microbiome
Authors: Kensara Anmar , Saito Hanae , Mongodin Emmanuel F. , Masri Radi 
Published: August 2023

AbstractPurposeTo characterize the microbiome composition in peri‐implant pocket of peri‐implantitis and peri‐implant sulcus controls using 16S rRNA gene sequencing.Materials and MethodsIn this controlled clinical cross‐sectional study, 23 subjects with control implants (n = 14) and diseased implants (peri‐implantitis, n = 21) were included. The peri‐implant pocket/sulcus was sampled and used to extract DNA and amplify the 16S rRNA gene using universal primers targeting the V3‐V4 regions. The resulting 16S PCR amplicons were sequenced on Illumina MiSeq, and the sequences were processed using DADA2 and the Human Oral Microbiome Database (HOMD) as references. Alpha and Beta diversity, as well as core microbiome and differential abundance analyses, were performed using the MicrobiomeAnalyst workflow.ResultsThere were no significant differences in microbial diversity between control implants and implants with peri‐implantitis (Shannon p = 0.82). Overall bacterial community structure assessed through beta diversity analysis was also not significantly different between the two groups (p = 0.18). However, high levels of Gram‐negative bacteria were detected in peri‐implant pockets compared to the control sulcus. Abundant species in peri‐implantitis were Capnocytophaga leadbetteri, Treponema maltophilum, Peptostreptococcus, Neisseria, P. gingivalis, and Porphyromonas endodontali, Lactococcus lactis and Filifactor alocis (p < 0.05). Gram‐positive bacteria such as Streptococcus salivaris, Prevotella melaninogenica, L. wadei, and Actinomyces spp. serve were more abundant in peri‐implant control sulcus.ConclusionsPeri‐implant sulcus in control implants harbors predominantly Gram‐positive bacteria, whereas pockets of implants with peri‐implantitis harbor predominantly Gram‐negative bacteria.
Quantification of temporomandibular joint space in patients with juvenile idiopathic arthritis assessed by cone beam computerized tomography
Authors: Otero María Florinda , Tahoces Pablo G. , Mera Antonio , Dalstra Michel , Verna Carlalberta , Pedersen Thomas Klit , Herlin Troels , Mira Jorge 
Published: August 2023

AbstractObjectiveTo describe a method to calculate the total intra‐articular volume (inter‐osseous space) of the temporomandibular joint (TMJ) determined by cone‐beam computed tomography (CBCT). This could be used as a marker of tissue proliferation and different degrees of soft tissue hyperplasia in juvenile idiopathic arthritis (JIA) patients.Materials and MethodsAxial single‐slice CBCT images of cross‐sections of the TMJs of 11 JIA patients and 11 controls were employed. From the top of the glenoid fossa, in the caudal direction, an average of 26 slices were defined in each joint (N = 44). The interosseous space was manually delimited from each slice by using dedicated software that includes a graphic interface. TMJ volumes were calculated by adding the areas measured in each slice. Two volumes were defined: Ve−i and Vi, where Ve−i is the inter‐osseous space, volume defined by the borders of the fossa and Vi is the internal volume defined by the condyle. An intra‐articular volume filling index (IF) was defined as Ve−i/Vi, which represents the filling of the space.ResultsThe measured space of the intra‐articular volume, corresponding to the intra‐articular soft tissue and synovial fluid, was more than twice as large in the JIA group as in the control group.ConclusionThe presented method, based on CBCT, is feasible for assessing inter‐osseus joint volume of the TMJ and delimits a threshold of intra‐articular changes related to intra‐articular soft tissue proliferation, based on differences in volumes. Intra‐articular soft tissue is found to be enlarged in JIA patients.
Intermittent ventricular pre‐excitation in symptomatic adults: Always a marker of low risk?
Authors: Robles Antonio Gianluca , Palamà Zefferino , Pernat Andrej , Gianfrancesco Domenico , Bartolomucci Francesco , Scarà Antonio , Borrelli Alessio , De Ruvo Ermenegildo , Calò Leonardo , Penco Maria , Romano Silvio , Sciarra Luigi 
Published: August 2023

AbstractBackgroundIntermittent ventricular pre‐excitation was considered a low‐risk marker for sudden death. However, to date, some studies do not exclude the existence of accessory pathways (APs) with high‐risk intermittent antegrade conductive properties. According to current European Guidelines, high‐risk features of APs are antegrade pathway conduction ≤250 ms in baseline or during the adrenergic stimulus, inducibility of atrioventricular reciprocating tachycardias (AVRT), inducibility of pre‐excited atrial fibrillation (AF), and presence of multiple APs. For all of these transcatheter ablation is recommended. The aim of our study was to evaluate the existence of differences in risk characteristics between patients with intermittent pre‐excitation (IPX) and those with persistent pre‐excitation (PPX), from a sample of adults with ventricular pre‐excitation and symptoms like palpitations.Methods293 adults [IPX: 51 (17.4%); PPX: 242 (82.6%)] underwent electrophysiological study and then catheter ablation of their APs if arrhythmia inducibility (AVRT/AF) was noted, or, conversely, if it was appreciated a fast AP antegrade conduction, in baseline or during intravenous isoproterenol infusion, or if multiple APs were detected.ResultsThere were no statistically significant differences in demographic characteristics (age and gender), AVRT/AF inducibility, antegrade conductive properties, the prevalence of multiple APs, and APs locations between IPX and PPX patients.ConclusionsIn our study, patients with IPX did not show significant differences in clinical and electrophysiological features versus PPX patients.