bluuebunny/crossref_metadata_2025_split huggingface dataset:
The clinical value of proneural, classical and mesenchymal protein signatures in WHO 2021 adult-type diffuse lower-grade gliomas
Authors: Dénes Anna , Bontell Thomas Olsson , Barchéus Hanna , Vega Sandra Ferreyra , Carén Helena , Lindskog Cecilia , Jakola Asgeir S. , Smits Anja 
Published: May 2023

Objectives
Accumulating evidence shows that mesenchymal transition of glioblastomas is associated with a more aggressive course of disease and therapy resistance. In WHO2021-defined adult-type diffuse gliomas of lower grade (dLGG), the transition of the tumor phenotype over time, has not been studied. Most efforts to correlate proneural, classical or mesenchymal phenotype with outcome in dLGG were made prior to the WHO 2021 classification. Here, we set out to investigate if phenotype predicted survival and tumor recurrence in a clinical cohort of dLGGs, re-classified according to the 2021 WHO criteria.

Methods
Using a TMA-based approach with five immunohistochemical markers (EGFR, p53, MERTK, CD44 and OLIG2), we investigated 183 primary and 49 recurrent tumors derived from patients with previously diagnosed dLGG. Of the 49 relapses, nine tumors recurred a second time, and one a third time.

Results
In total, 71.0% of all tumors could be subtyped. Proneural was most dominant in IDH-mut tumors (78.5%), mesenchymal more common among IDH-wt tumors (63.6%). There was a significant difference in survival between classical, proneural and mesenchymal phenotypes in the total cohort (p<0.001), but not after molecular stratification (IDH-mut: p = 0.220, IDH-wt: p = 0.623). Upon recurrence, proneural was retained in 66.7% of the proneural IDH-mut dLGGs (n = 21), whereas IDH-wt tumors (n = 10) mainly retained or gained mesenchymal phenotype. No significant difference in survival was found between IDH-mut gliomas remaining proneural and those shifting to mesenchymal phenotype (p = 0.347).

Conclusion
Subtyping into classical, proneural and mesenchymal phenotypes by five immunohistochemical markers, was possible for the majority of tumors, but protein signatures did not correlate with patient survival in our WHO2021-stratified cohort. At recurrence, IDH-mut tumors mainly retained proneural, while IDH-wt tumors mostly retained or gained mesenchymal signatures. This phenotypic shift, associated with increased aggressiveness in glioblastoma, did not affect survival. Group sizes were, however, too small to draw any firm conclusions.
Breast cancer-related lymphedema and recurrence of breast cancer: Protocol for a prospective cohort study in China
Authors: Zhuang Linli , Chen Qian , Chen Huaying , Zheng Xuemei , Liu Xia , Feng Zhenzhen , Wu Shaoyong , Liu Li , Shen Xiaolin 
Published: May 2023

Introduction
The primary aim is to determine the factors associated with breast cancer-related lymphedema and to identify new associated factors for the recurrence of breast cancer and depression. The secondary objective is to investigate the incidence of breast cancer-related events (breast cancer-related lymphedema, recurrence of breast cancer, and depression). Finally, we want to explore and validate the complex relationship among multiple factors influencing breast cancer complications and breast cancer recurrence.

Patients and methods
A cohort study of females with unilateral breast cancer will be conducted in West China Hospital between February 2023 and February 2026. Breast cancer survivors in the age range of 17–55 will be recruited before breast cancer surgery. We will recruit 1557 preoperative patients with a first invasive breast cancer diagnosis. Consenting breast cancer survivors will complete demographic information, clinicopathological factors, surgery information, baseline information, and a baseline depression questionnaire. Data will be collected at four stages: the perioperative stage, chemotherapy therapy stage, radiation therapy stage, and follow-up stage. Data including the incidence and correlation of breast cancer-related lymphedema, breast cancer recurrence, depression, and medical cost will be collected and computed through the four stages above. For every statistical analysis, the participants will be classified into two groups based on whether they develop secondary lymphedema. Incidence rates of breast cancer recurrence and depression will be calculated separately for groups. Multivariate logistic regression will be used to determine whether secondary lymphedema and other parameters can predict breast cancer recurrence.

Discussion
Our prospective cohort study will contribute to establishing an early detection program for breast cancer-related lymphedema and recurrence of breast cancer, which are both associated with poor quality of life and reduced life expectancy. Our study can also provide new insights into the physical, economic, treatment-related and mental burdens of breast cancer survivors.
Identifikasi Molekuler Chlorella sorokiniana menggunakan Marka ITS dan 18S rDNA serta Produksi Karotenoid dengan Perlakuan Cahaya
Authors: Zulkarnain Muhammad Iskandar , Kusumaningrum Hermin Pancasakti , Nurhayati Nurhayati , Suprihadi Agung , Zainuri Muhammad 
Published: May 2023

Chlorella sorokiniana merupakan salah satu mikroalga penghasil astaxantin. Produksi astaxantin C. sorokiniana dapat meningkat pada kondisi kultur yang optimal. Penelitian ini bertujuan untuk memastikan identitas C. sorokiniana secara molekuler menggunakan ITS, dan 18S rDNA, serta untuk mengetahui pertumbuhan dan produksi astaxantin C. sorokiniana berdasarkan perlakuan cahaya. Metode yang digunakan pada penelitian ini meliputi kultivasi mikroalga C. sorokiniana, isolasi DNA, uji kuantitatif DNA, identifikasi molekuler melalui proses amplifikasi DNA dengan PCR (Polymerase Chain Reaction) menggunakan marka molekuler ITS dan 18S rDNA, visualisasi hasil PCR dengan elektroforesis menggunakan GelDoc diikuti sekuensing DNA. Produksi astaxantin mikroalga C. sorokiniana dihitung selama 10 hari dibawah perlakuan cahaya matahari dan sinar UV. Identifikasi molekuler C. sorokiniana menggunakan ITS memperoleh dengan ukuran fragmen sebesar 500 pb, sedangkan fragmen 18S rDNA sebesar 600 pb. Pertumbuhan mikroalga C. sorokiniana kontrol pada hari ke-10 dengan kerapatan 15×106 sel/ml, sedangkan pada perlakuan cahaya matahari dan sinar UV pada hari ke-10 dengan kerapatan 38,45×106 sel/ml. Konsentrasi astaxantin yang dihasilkan mikroalga C. sorokiniana pada perlakuan kontrol mencapai tertinggi 0.3306 mg/mL dan pada perlakuan cahaya matahari dan sinar UV meningkat mencapai 0,3874 mg/mL. Chlorella sorokiniana is one of the astaxanthin-producing microalgae. Astaxanthin production of C. sorokiniana can be increased under optimal culture conditions. This study aims to determine the molecular identity of C. sorokiniana using ITS and 18S rDNA, as well as to determine the growth and production of astaxanthin C. sorokiniana based light treatment. The methods used in this study included C. sorokiniana microalgae cultivation, DNA isolation, DNA quantitative testing, molecular identification through the process of DNA amplification with PCR (Polymerase Chain Reaction) using ITS and 18S rDNA molecular markers, visualization of PCR results by electrophoresis using GelDoc followed by sequencing DNA. The astaxanthin production of C. sorokiniana microalgae was calculated for 10 days under sunlight and UV light treatment. Molecular identification of C. sorokiniana using ITS obtained a fragment size of 500 bp, while the 18S rDNA fragment was 600 bp. The growth of C. sorokiniana microalgae was controlled on day 10 with a density of 15×106 cells/ml, whereas in the treatment of sunlight and UV light on day 10 with a density of 38,45×106 cells/ml. The concentration of astaxanthin produced by C. sorokiniana microalgae in the control treatment reached 0,3306 mg/mL and in the sunlight and UV light treatments it increased to 0,3874 mg/mL.
Validation of the Eating Habits Questionnaire in Greek adults
Authors: Bali Georgia , Kokka Ioulia , Gonidakis Fragiskos , Papakonstantinou Eleni , Vlachakis Dimitrios , Chrousos George P. , Kanaka-Gantenbein Christina , Bacopoulou Flora 
Published: May 2023

Healthy eating has gained ground in people's daily lives in modern society. However, an overwhelming preoccupation with healthy eating can lead to a pathological form setting the ground for orthorexia nervosa. This study aimed to validate the Greek version of the Eating Habits Questionnaire (EHQ) in adults 18 to 65 years old. The EHQ evaluates orthorexia nervosa traits. An online survey was conducted among adults of the general Greek population by administrating a battery of self-report instruments. The IPIP Big-Five personality questionnaire, Beck’s Depression Inventory, the Obsessive-Compulsive Inventory-Revised, the Bulimic Investigatory Test, the Edinburg BITE, and the Eating Attitudes Test-13 were used. Internal consistency, test-retest reliability, and convergent and criterion validity were examined. A total of 551 adults (92.2% females) voluntarily participated in the study. Results suggest that the Greek version of the instrument has good psychometric properties. Analysis revealed a 3-factor model explaining 48.20% of the total variance. Cronbach’s alphas ranged between 0.80 to 0.82, indicating good internal consistency. The test-retest reliability analysis showed no statistically significant difference between the measurements of the first and the post-2 weeks. Correlations with other eating disorder-related constructs were found to be weak to moderate. Body mass index was not significantly correlated with neither of the three EHQ subscales. The Greek version of EHQ is a robust instrument that could be used in clinical practice and research in the field of eating disorders in Greece.
Testing the relationship between the technical condition of motorcycle shock absorbers determined on the diagnostic line and their characteristics
Authors: Podosek Krzysztof , Jaśkiewicz Marek , Zuska Andrzej 
Published: May 2023

Abstract
The article presents the results of research on the relationship between the technical condition of motorcycle shock absorbers determined on the diagnostic line and their damping characteristics. The basic division of shock absorber tests was characterized, which includes tests of shock absorbers installed in the motorcycle and disassembled, carried out on a test stand made of MTS components. The results of two sets of shock absorbers tested using the EUSAMA and THETA methods, as well as tested on a stand for testing shock absorbers removed from a motorcycle, are discussed. Based on the obtained results, the best method of diagnosing shock absorbers was indicated.
The prevalence of smoking cigarettes among HIV-infected patients after a decade. Single-center analysis
Authors: Tochman Pola , Mikuła Tomasz , Lembas Agnieszka , Wiercińska-Drapało Alicja 
Published: May 2023

Abstract

Objectives
Smoking habit is known to be a risk factor for the development of multiple diseases and conditions, premature death, and worse quality of life. The prevalence of smoking in PLWH is 2–3 times higher than in the general population. The study aimed to evaluate how the prevalence of smoking has changed among PLWH over the past decade.

Methods
The data of n=204 PLWH hospitalized from November 2018 to November 2019 was analyzed. All patients filled out the survey including age, gender, the number of cigarettes smoked, the number of years as a smoker, and the impact of HIV diagnosis on the number of cigarettes smoked. The data was compared to a similar analysis performed in our department in 2009.

Results
The study showed a decrease in the prevalence of smoking among PLWH over the past decade. In comparison to 2009, a statistically significant (p<0.05) reduction in the number of smoking individuals among ever and never smokers was observed both in males and in females.

Conclusions
The prevalence of smoking cigarettes among PLWH in our department has significantly decreased since 2009 but remains much higher than in the general population. Smoking cessation interventions provided by HIV care professionals are necessary and should be continued among PLWH.
A global optimal resource scheduling algorithm in muti-layer optical networks
Authors: Zhang Lei , Chen Guangxuan , An Jing , Tian Li 
Published: May 2023

Abstract
This paper analyzed the characteristic of business flow and divided the business flow into two categories according to the flow distribution: elephant flow and mice flow, combined with the characteristics of the elephant flow’s high delay tolerance and bandwidth demand flexibility, focused on the multi-layer traffic coordination problem in the optical transport network, and the lack of fairness guarantee and multi-path transmission mechanism in the competition of bandwidth resources between elephant flows, realized the coordination of service traffic between the IP layer and the optical layer, proposed a periodic resource scheduling mechanism and a cross-layer global routing and bandwidth scheduling algorithm based on fairness and multi-path transmission under the unified control “IP over OTN” transport network architecture based on the SDN architecture and the OpenFlow protocol.
Two-dimensional material integrated micro-nano fiber, the new opportunity in all-optical signal processing
Authors: Wang Xinyu , Ma Wanzhuo , Fu Yanwei , Liu Xianzhu , Tao Zonghui , Song Yansong , Dong Keyan , Jiang Huilin 
Published: May 2023

Abstract
With the development of all-optical networks, all-optical devices have become a research hotspot in recent years. Two-dimensional materials, represented by graphene and black phosphorus, have attracted great interest in the scientific community due to their excellent optical, electrical, magnetic, and mechanical properties. Bridging the gap between fiber optics and nanotechnology, microfibers can interact with light and matter at the micro or even nanoscale. By combining two-dimensional materials with microfibers, composite waveguides can be formed. They have the advantages of high nonlinear effect, all-fiber structure, and high damage threshold, etc. The composite waveguide can be directly applied to optical fiber communication systems, and plays an important role in the field of all-optical signal processing with a huge application prospect. In this review, the properties of typical 2D materials are first introduced. Next, the preparation methods of the relevant equipments are introduced and compared. Then, the all-optical signal processing technology based on 2D material-integrated microfiber composite waveguide is reviewed. The latest developments of all-optical modulators, all-optical wavelength converters, all-optical logic gates and all-optical thresholding devices are presented. Finally, the challenges and opportunities for the future development of 2D materials-integrated microfiber optoelectronic devices are summarized.
Bioinformatics analyses suggest that mutations in COL12A1 and its miRNAs promote stomach adenocarcinoma via loss of COL12A1 suppression
Authors: Xin Qiang , Zhao Yanlong , Huang Shaoli , Xin Hong-Yi , Feng Zhipeng , Xin Hong-Wu , Wang Xiaoyan , Wang Yingying , Su Xiulan 
Published: May 2023

Abstract

Objectives
Stomach adenocarcinoma (STAD) is the fifth most common cancer and the third leading cause of cancer-related deaths worldwide. It is important to understand its molecular basis better and identify noninvasive biomarkers for targeted therapy. Usually, tumors contain driver genes or passenger genes, which can be regulated by microRNAs (miRNAs). However, the mechanism underlying the interaction between miRNA and their target genes in STAD has not been fully demonstrated. To date, no miRNA-targeted gene mutation sites that promote STAD progression have been reported, and there are no reports of the mutations within the 3′-UTR of COL12A1 that could contribute to STAD development.

Methods
Here, multiple genomic and transcriptomic profiles of STAD patients were downloaded from several reputable online databases and various analyses were conducted, including assessments of mutation rates, copy number variations (CNVs), single‐nucleotide polymorphisms (SNPs), RNA secondary structure, protein-protein interaction (PPI), pathway network, differentially expressed genes (DEGs), and patient survival rate. We aimed to evaluate impact of the SNPs on progression of stomach adenocarcinoma.

Results
We identified 22 critical DEGs involved in cell-cell and cell-matrix interaction. Seven of these key DEGs, including COL12A1, were associated with poorer overall survival. Interestingly, we found that COL12A1 was targeted by the miRNAs: hsa-miR-130b-5p (which carries the mutations rs1174814901 and rs761813865) and hsa-miR-18a-5p (which carries the mutation rs1198460870). These mutations lead to a change in the secondary structure of the miRNAs. And we also found that the miRNA target sites of COL12A1 were also mutated. These mutations at both miRNAs and COL12A1 likely attenuated their binding capability, leading to compensatory miRNA overexpression in the STAD patients. The miRNA overexpression was further found to be significantly associated with worse survival in STAD patients.

Conclusions
Our results suggest that mutations in Col12A1 and its miRNAs promote STAD via loss of COL12A1 suppression, providing new insight into the molecular mechanisms of STAD progression. This finding has significant implications for the development of novel noninvasive diagnostic, prognostic and therapeutic biomarkers.
Monocytes differentiate along two alternative pathways during sterile inflammation
Authors: Villar Javiera , Ouaknin Léa , Cros Adeline , Segura Elodie 
Published: May 2023

AbstractDuring inflammation, monocytes differentiate within tissues into macrophages (mo‐Mac) or dendritic cells (mo‐DC). Whether these two populations derive from alternative differentiation pathways or represent different stages along a continuum remains unclear. Here, we address this question using temporal single‐cell RNA sequencing in an in vitro model, allowing the simultaneous differentiation of human mo‐Mac and mo‐DC. We find divergent differentiation paths, with a fate decision occurring within the first 24 h and confirm this result in vivo using a mouse model of sterile peritonitis. Using a computational approach, we identify candidate transcription factors potentially involved in monocyte fate commitment. We demonstrate that IRF1 is necessary for mo‐Mac differentiation, independently of its role in regulating transcription of interferon‐stimulated genes. In addition, we describe the transcription factors ZNF366 and MAFF as regulators of mo‐DC development. Our results indicate that mo‐Macs and mo‐DCs represent two alternative cell fates requiring distinct transcription factors for their differentiation.
Improved Accuracy of Naïve Bayes Algorithm and Support Vector Machine Using Particle Swarm Optimization for Menstrual Cup Sentiment Analysis on Twitter
Authors: Shalikha Dini , Alamsyah Alamsyah 
Published: May 2023

Menstrual cup is a menstrual hygiene sanitation tool that replaces disposable sanitary napkins for women that reaps many pros and cons in its use. From this, it is necessary to analyze the public's views regarding the use of menstrual cups, which is called sentiment analysis. Sentiment analysis is a process that aims to determine the polarity of the sentiment of a text. This paper performs a classification of menstrual cup sentiment analysis on Twitter using the Naïve Bayes and the Support Vector Machine algorithm. Particle Swarm Optimization is applied to improve the accuracy of both classification algorithms. The final result of the accuracy obtained by the Naïve Bayes algorithm is 92.72% and the Support Vector Machine algorithm is 96.13%. While the accuracy results after Particle Swarm Optimization is applied, for Naïve Bayes it produces an accuracy rate of 95.87%, and Support Vector Machine is 96.68%.
Implementation Data Mining with Naive Bayes Classifier Method and Laplace Smoothing to Predict Students Learning Results
Authors: Pradana Dany , Sugiharti Endang 
Published: May 2023

Abstract. The application of information technology in the field of education produces big data. It retains information that can be treated as useful. Having data mining, can be used to model highly useful student performance for educators performing corrective actions against weak students.
Purpose: The study was to identify the application and accuracy algorithm Naive Bayes Classifier to predict students' study results.
Methods: The prediction system for student learning outcomes was built using the Naive Bayes Classifier and Laplace Smoothing methods using a combination of two Information Gain and Chi Square feature selections. The experiment was carried out 2 times using different dataset comparisons.
Result: In the first experiment using a dataset of 80:20, the accuracy Naive Bayes Classifier method with Laplace Smoothing and without Laplace Smoothing showed the same results as 94.937%. On the second experiment to equate dataset 60:40 results of the Naive Bayes Classifier accurate method without Laplace Smoothing only 86.076%, then score a 91.772% accuracy using the Laplace Smoothing. The improvement is caused by a probability of zero that can be worked out with Laplace Smoothing.
Novelty: The selection feature process is very important in the classification process. Thus, in this study, information gain and chi square double selections of such features as information gain and so promote accuracy.
On semantic redundancy in the epigraphs of Moesia Superior
Authors: Kunčer Dragana 
Published: May 2023

AbstractIn this paper, the principle of semantic redundancy is examined as evidenced in the corpus of the Moesia Superior inscriptions (Inscriptions de la Mésie supérieure – IMS). The objective is to identify pleonastic phrases within the epigraphic material and to distinguish instances of unmotivated redundancy. The starting point is the observation made by Hofmann, Löfstedt, Herman, and others that this type of redundancy, contrary to the pragmatically motivated or “rhetoric” one, could reflect the ongoing semantic change in Vulgar Latin. Given the scarcity of semantic redundancy findings in the IMS corpus, this paper also serves as an introduction to this linguistically important topic.
Sound, Interrogation, Torture: John le Carré and the Audible State
Authors: Hepburn Allan 
Published: May 2023

AbstractWhile completing his national service from 1951 to 1952, John le Carré served as an intelligence corps officer whose duties included the interrogation of refugees; as a member of MI5 and MI6 between 1958 and 1963, he interrogated defectors from Soviet bloc countries to test their sincerity or duplicity. In Tinker, Tailor, Soldier, Spy, The Honourable Schoolboy, Smiley's People, The Secret Pilgrim, The Mission Song, and le Carré's other novels, interrogation scenes contribute to the total soundworld of the audible state. As a way to gather information through extorted speech, interrogation occurs in extraterritorial nonplaces or undisclosed, deniable locations. Drawing on historical documents, this essay positions interrogation in terms of torture, human rights, and the capacity of the state to inflict harm or to extend protection to individuals under its authority. In le Carré's novels, characters not only listen like states—comprehensively, omnisciently—but also begin to think like states.