bluuebunny/crossref_metadata_2025_split huggingface dataset:
Lab-In-A-Fiber Detection and Capture of Cells
Authors: Varela João C. , Harish Achar V. , Maniewski Pawel , Gibbon Timothy , Tudoran Oana , Heuchel Rainer , Löhr Matthias , Margulis Walter , Russom Aman , Laurell Fredrik 
Published: January 2025

Abstract

A lab-in-a-fiber component was fabricated using an optical fiber and a fiber capillary. It was used in a test suspension of fluorescently labeled and unlabeled cells and enabled detection of the labeled cells. Subsequently the labeled cells were selectively collected via suction into the capillary. A novel sampling technique reduced photobleaching of the labeled cells, extending the measurement time. The collected cells remained viable for downstream analysis. This platform’s low fabrication cost, simplicity, compatibility with standard laboratory equipment, and capacity for fully automated cell capture highlights its potential for future applications in minimally invasive sample collection and point-of-care diagnostics.
WITHDRAWN: SUV39H1 alleviates MAFLD through inhibition of the FABPs/CEPT1 signaling pathway
Authors: sun yi , Wang changyuan , Wang Yang , Hao Ru , Liu Lu , Jin yue , Sun Huijun 
Published: January 2025

Abstract

The full text of this preprint has been withdrawn, as it was submitted in error. Therefore, the authors do not wish this work to be cited as a reference. Questions should be directed to the corresponding author.
Using Cumulative summation analysis (CUSUM) for the learning curve of robotic docking time in radical prostatectomy with the HUGO RAS System
Authors: Russo Pierluigi , Sighinolfi Mariachiara , Mastrovito Sara , Cretì Antonio , Panico Giovanni , Marino Filippo , Presutti Simona , Scarciglia Eros , Gandi Carlo , Ragonese Mauro , Gavi Filippo , Sacco Emilio , Foschi Nazario , Rocco Bernardo Maria Cesare 
Published: January 2025

Abstract

Minimally invasive surgery like robotic surgery is known to yield better outcomes in terms of blood loss, blood transfusion, and length of stay, and robot-assisted radical prostatectomy provides a clear example compared to open surgery. It is still constrained by issues related to platform availability and cost-effectiveness. Introducing new robotic platforms, such as the HUGO™ Robot-Assisted Surgery (RAS) System, could lead to longer operating times caused by the surgeon's learning curve, system configuration, adjustment of robotic devices, and robotic docking. Several studies have assessed the influence of resident physicians on outcomes in urological surgeries. Our main objective was to evaluate the learning curve of the docking time for 195 radical prostatectomies performed in our hospital. The results of our research indicate that the setup and docking process with the HUGO RAS system can be accomplished with ease, and the learning curve for robotic docking is consistent with the available data for other robotic platforms. Our training facilitated a rapid docking process and seamless completion of the surgery.
Omics studies of nuclear protein aggregates in subcellular fractions reveals co- aggregation of RNA-binding proteins affecting cytosolic pathways
Authors: Shademan Milad , Flannery Sarah , Bos Erik , Evers Tom , Sheikhhassani Vahid , Mashaghi Alireza , Kusters Benno , Engelen Baziel , Sharp Thom , Fischer Roman , Kessler Benedikt M. , Raz Vered 
Published: January 2025

Abstract

Disease-associated RNA binding protein (RBP) aggregation is a hallmark of several age-related neurodegenerative diseases. How insoluble RBP aggregates leads to cellular dysfunction is poorly understood. Here, we investigated the molecular mechanisms affected by insoluble PABPN1 aggregates. PABPN1 aggregates are nuclear, but PABPN1 regulates nuclear export of mRNA. To explore the cellular consequences of PABPN1 nuclear aggregates, we performed RNA sequencing and proteomic studies in subcellular fractions in an inducible human muscle cell model. RNA sequencing analyses revealed PABPN1 dysfunction in this cell model associated with reduced endogenous PABPN1 levels. Proteomic analyses revealed that most of the changes driven by PABPN1 nuclear aggregates were in the cytoplasmic fraction, accounting for reduced cell metabolism, muscle cell differentiation and muscle cell biomechanics. Changes in the insoluble fraction were small but enriched for RBPs. We show that sequestration of mRNA in nuclear aggregates is associated with impaired nuclear export of mRNA and reduced translational efficiency. Our study suggests that RBPs nuclear protein aggregates are regulated by both gain-of-function and loss-of-function mechanisms, which is relevant for the development of therapeutics for age-associated protein aggregation diseases.
Evaluation of Robotic Surgery in Pediatric and Adolescent Populations for Posterior Approach Instrumentation - a Systematic Review, Single-Arm Meta-Analysis and Meta-Regression
Authors: Łajczak Paweł , Łajczak Anna 
Published: January 2025

Abstract

Purpose
Robot-assisted (RAS) surgery is increasingly used in pediatric and adolescent posterior-approach spine surgery. However, current literature often focuses on pairwise comparisons, limiting comprehensive evaluation of robotic effectiveness across diverse conditions. This single-arm meta-analysis aimed to evaluate the effectiveness of RAS in pediatric and adolescent spine surgery.
Methods
A systematic search was conducted across PubMed, Embase, Scopus, Web of Science, and Cochrane Library from inception to December 2024. Studies were included if they reported outcomes of RAS for pedicle screw placement in patients under 25 years with spinal deformities. The primary outcome was the accuracy of pedicle screw placement, assessed using the Gertzbein and Robbins (GR) grading system. Secondary outcomes included intraoperative metrics and deformity correction. A random-effects model was applied for meta-analyses.
Results
Fifteen studies encompassing 13,325 screws and 920 patients were included. The pooled rate of clinically acceptable screws (GR A+B) was 95.66% (95% CI: 94.04–97.28), with 88.54% (95% CI: 83.60–93.48) achieving perfect placement (GR A). The incidence of heavily misplaced screws (GR D+E) was 0.59% (95% CI: 0.18–1.16). Blood loss averaged 687.85 mL (95% CI: 534.27–841.43), and the mean operation time was 306.03 minutes (95% CI: 273.19–338.88). Curve correction rates and Cobb angle improvements were consistent with existing scoliosis treatment benchmarks. Heterogeneity was observed, likely due to variability in study designs, robotic systems, and patient populations.
Conclusion
RAS demonstrates high accuracy and safety in pediatric and adolescent spine surgery, with low rates of screw misplacement and acceptable secondary outcomes. While these findings highlight the clinical benefits of robotics, high heterogeneity and limited long-term data necessitate further research. Standardized reporting, longitudinal studies, and comparative analyses are essential to fully realize the potential of robotic systems in pediatric spine surgery.
Assessing acceptability of digital financial services and associated factors, opportunities and challenges in Burundi health sector
Authors: Niragira Juste , Ntawuyamara Epipode , Icitegetse Armella , Niyukuri Janvier 
Published: January 2025

Abstract

Background: Digital financial services (DFS) allow people to send, receive payments, and manage their finances. In Burundi, they are limited to individual money transfer agents and the purchase of phone credits.
Objective: To assess the acceptability of digital payment (DP) and identify factors, opportunities and challenges associated to the use of DFS in Burundi health system.
Methods: This is a cross-sectional, descriptive and analytical study conducted in Burundi from March to November 2023. The study was conducted in 16 health districts and included 2731 health care and services providers (HPs).
Results: The majority of HPs was young and working in the rural area. Most of them (86.5% ) had a general knowledge on DP. The acceptance level of DP among the HPs was 62% against 74.8% among the community health workers (CHWs). Many of our study participants (88.1%) believed that DFS have a positive impact on vaccination campaigns and 74.9% thought it has an excellent effectiveness. The HPs who worked at the rural level were 1.26 times more likely to use DP during vaccination campaigns than those in the urban area. The CHWs and paramedical staff were respectively 3.94 and 1.48 times more likely to use DP during vaccination campaigns than physicians.
Conclusion: This study sufficiently proved that the use of DP in the Burundi health sector in general and during vaccination campaigns especially can have a positive impact.
Volcano Monitoring System for Long-Term Eruption Forecasting Using Multiple Data Sources
Authors: Tsuji Takeshi , Ideno Minoru 
Published: January 2025

Abstract

Accurately forecasting volcanic eruptions is challenging due to the complexity of precursory signals. Here, we develop a machine learning-based long-term eruption forecasting model for Mount Aso, Japan, by integrating multiple observational datasets—seismic tremors, magnetic field, crater wall temperature, thermal pool temperature and volume, tilt, and volcanic gas amount—at the characteristic temporal scales of the underlying physical phenomena. The temporal scales are aligned with the intrinsic dynamics captured by each dataset to enhance the model's predictive capability. We construct a theoretical framework to quantify the predictive performance improvement. Our proposed model significantly improves predictive performance, increasing the Matthews correlation coefficient by 0.65 compared to the conventional seismic-tremor-based model, and achieving a precision of >70% in predicting volcanic eruptions. Our findings demonstrate that an ensemble of multiple data sources over optimized temporal scales, underpinned by a theoretical ensemble framework, enables high-precision, interpretable eruption forecasts months in advance and makes effective disaster mitigation planning possible.
Somatic Mutations and Outcomes in CML Adolescent and Young Adults Compared to Children, Adults, and BCR::ABL1-positive ALL Patients
Authors: Polakova Katerina Machova , Krizkova Jitka , Polivkova Vaclava , Laznicka Adam , Curik Nikola , Benesova Adela , Suchankova Pavla , Smazik Tomas , Vysinova Veronika , Mikulenkova Dana , Klamova Hana , Markova Marketa Stastna , Srb Dana , Zuna Jan , Zaliova Marketa , Trka Jan , Salek Cyril 
Published: January 2025

Abstract

Adolescent and young adult (AYA) patients with chronic myeloid leukemia in chronic phase (CML-CP) reportedly fare worse on tyrosine kinase inhibitor (TKIs) than adults. This real-life study compared mutation profiles and outcomes in 80 AYA, 97 adult, and 16 pediatric CML-CP patients, alongside 81 BCR::ABL1-positive acute lymphoblastic leukemia (ALL) patients. Somatic mutations in cancer-related genes (CRGs) were more frequent in AYAs with CML-CP (25.0%) than in adults (19.6%) or children (12.5%). AYAs with Ph+ ALL also exhibited higher mutational frequencies (53.3%) compared to children (26.7%) and adults (38.9%). Mutation landscapes differed at diagnosis with ASXL1, DNMT3A, and TET2dominant in CML-CP and RUNX1, IKZF1, and BCR::ABL1predominated in Ph+ ALL. ASXL1 mutations correlated with reduced progression-free survival (PFS) in AYAs and adults, with adults showing increased BCR::ABL1 mutations during TKI therapy, a trend not observed in AYAs. Nilotinib improved PFS in AYAs with ASXL1 mutations, highlighting the efficacy of higher-generation TKIs. ASXL1 mutations also impaired erythropoiesis, warranting further validation. Despite a higher mutational burden, AYAs did not show worse prognoses than adults, with lower mutation rates at follow-up suggesting better adherence. Mutation profiling and optimized TKI use are crucial to mitigate progression risks in CRG-mutated patients.
Urban Forest Quality Corresponds with Soil Microbial Community Composition and Arbuscular Fungi Root Colonization
Authors: Yarwood Stephanie , Gaimaro Lindsay , Gonzalez Humberto Castillo 
Published: January 2025

Abstract

Fairfax County government in Virginia, USA, conducted an extensive survey of urban forests that included assessing tree health, quantifying invasive species, and determining impervious surface. These measurements were used to calculate a quality index using the iTree tool kit. Building on survey results, our research team sampled soils and tree roots in a subset of sites representing a range of forest quality index values. Soil bacterial/archaeal and fungal communities were quantified and characterized using qPCR and amplicon sequencing to understand how soil biology relates to aboveground forest health. We observed differences in community composition and putative functional assignments indicated a decrease in ectomycorrhizal fungi with declining quality. Arbuscular mycorrhizal fungal root colonization also decreased with forest quality. This study demonstrates the crucial above- and belowground connections within urban forests and highlights the need for urban managers to consider soil biology when assessing urban ecosystem health.
Spinal Cord Stimulation for Neuropathic Pain Caused by Spinal Cysticercosis: Case Report
Authors: Guo Mali , Zhang Yixin , Li Meirui , Zhang Guangjian 
Published: January 2025

Abstract

Background:Spinal cord cysticercosis, a rare subtype of neurocysticercosis (NCC), primarily affects the subarachnoid space. The optimal treatment approach remains uncertain, with both pharmacological and surgical strategies being employed. Spinal cord stimulation (SCS) has been established as a safe and effective therapy for chronic pain,we present a case of neuropathic pain caused by spinal cord cysticercosis, successfully managed with SCS.
Case presentation:A 69-year-old female presented with an 8-year history of trunk and limb pain. Eight years ago, she was diagnosed with spinal cord cysticercosis at a hospital in South Korea and underwent laminectomy. The surgery was uneventful, and her postoperative recovery was satisfactory. However, the pain persisted and progressively worsened over time, eventually involving the entire trunk and limbs, accompanied by sensory abnormalities and progressive lower limb weakness. After a thorough evaluation based on her symptoms, an electrode was placed at the L2 segment for spinal cord stimulation. During hospitalization, stimulation parameters were adjusted multiple times based on patient feedback. At a one-week postoperative follow-up, the patient reported significant relief from trunk and limb pain, along with marked improvement in lower limb strength, sensory perception, numbness, and cold sensations.
Conclusions: This case demonstrates the efficacy of SCS in treating neuropathic pain caused by spinal cord cysticercosis. The benefits extend beyond pain relief to include recovery of impaired motor function, providing a valuable reference for managing refractory pain in similar patients.
Evaluation of Antifungal Efficacy of Lantana camara, Hyptis suaveolens, and Trichoderma viride against Fungal Biodegradation of Timber
Authors: S Bharath Kumar , Kumar Muthu , Mamatha N , Nagadesi Praveen Kumar 
Published: January 2025

Abstract

Wood-rotting fungi (WRF) are significant agents of wood degradation. While chemical preservatives have been traditionally effective, this study investigates eco-friendly alternatives using antagonistic fungi and plant extracts as biological control agents (BCAs). The biodegradation potential of seven white-rot fungi—Trametes hirsuta, Schizophyllum commune, Pycnoporus sanguineus, Lenzites betulinus, Ganoderma applanatum, Oligoporus placentus, and Fomes meliae—were assessed on five wood types: Artocarpus heterophyllus, Pterocarpus marsupium, Tectona grandis, Dalbergia latifolia, and Santalum album. The effectiveness of BCAs was evaluated through a wood preservation technique conducted on selected woods mentioned above. The antagonistic effect of Trichoderma viride and Plant extract (Ethanolic & Methanolic) of Lantana Camera and Hyptis suaveolens (0.5–2.5%) were investigated against the T. hirsuta, S. commune, P. sanguineus, L. betulinus, G. applanatum, O. placentus and F. melia species of WRF. Degradation rates of A. heterophyllus, P. marsupium, and T. grandis reached 53.3%, 49.8%, and 47.2%, respectively. Methanolic extracts of Lantana camara and Hyptis suaveolens at 2.5% concentration demonstrated more antifungal efficacy, inhibiting fungal growth by 95–100%. The antagonistic fungus Trichoderma viride demonstrated 89% inhibition of pathogenic WRF. Wood preservation technique revealed 2.5% methanolic extracts of H. suaveolens able to control the WRF when compare to L. camara methonolic extract. This study highlights the potential of plant-derived extracts and antagonistic fungi as eco-friendly alternatives to chemical preservatives, addressing both fungal degradation and environmental concerns.
Analysis of shear creep behaviors of hardwood and softwood using creep recovery curves
Authors: Shimazaki Kanon , Ando Kosei 
Published: January 2025

Abstract

This study was aimed at exploring the sheer creep behavior of wood through off-axis tensile creep and creep recovery tests. Using the creep recovery data, the shear creep properties of softwood (Japanese Hinoki cypress, Chamaecyparis obtusa) and hardwood (Japanese Buna beech, Fagus crenata) were compared. The trends of three components of strain, i.e., instantaneous elastic, delayed elastic, and permanent strains, during shear creep were predicted by decomposing the total strain during creep recovery, assuming that the rate of increase in delayed elastic strain is the same as the recovery rate during creep recovery. Fitting a Burger model to each predicted strain yielded more reliable material parameters compared with those obtained by simply mathematically fitting the Burger model to the total creep strain. The Burger model demonstrated excellent accuracy in fitting the measured creep curves of hardwood. However, it could not explain the shear creep behavior of softwood. This discrepancy in the fitting results was attributable to the differences in the behavior of permanent strain: The permanent strain of cypress exhibited a curvilinear trend, while that of beech displayed a more linear trend. To explain the curvilinear behavior of permanent strain, a modified Burger model, which assumes that the apparent viscosity of permanent strain changes in a strain-rate-dependent manner, was proposed. The modified Burger model yielded better fitting results than the conventional Burger model, suggesting that the viscous component of wood exhibits an apparent viscosity that depends on the strain rate rather than a constant value, as assumed in the conventional Burger model.
Assessing the Impact of NPK Application Rates at different Growth Stages on morphology and Biomass Yield of Tephrosia vogelii Hook. f. (Fabaceae
Authors: Effa Emmanuel Bassey , Bisong Benedette Ozoemena , Okweche Simon Idoko , Binang Walter Bisong 
Published: January 2025

Abstract

A two-year field study investigated the effects of varying rates of fertilizer NPK 20.10.10 and timing of application on the vegetative growth and biomass yield of T. vogelii (Fish bean) at the study area in 2019 and 2020 cropping seasons. Treatments consisted of three rates of N.P.K 20.10.10. (0, 100 and 200 kg/ha) and two fertilizer application periods (pre-anthesis and at pod commencement), giving a combination of 3 x 2 x 3 = 18 treatments. Randomized Complete Block Design (RCBD) with three replications was used to disperse all treatments in the field. Results showed that T. vogelii plants that were fertilized pre- anthesis with 200 kg/ha N.P.K. 20.10.10 in both years had higher growth and yield performance, outstripping plants that received 0 and 100 kg/ha NPK. Interactively, 200 kg/ha of NPK fertilizer at early pre-anthesis gave the best performance of the plant during the study. The study demonstrates that T. vogelii is suitable propagated in the study area and inorganic fertilizer at 200 kg/ha NPK is optimum for basal fertilization.
Development of Filament Dryer for Drying Process of 3D Printing Filament in Open Storage Condition
Authors: Widyianto Agus 
Published: January 2025

Abstract

PLA (Polylactic Acid) filament is one of the most popular 3D printing filaments, especially in industrial applications. This can happen because PLA is hygroscopic and easily reacts with water, breaking the chain and reducing its mechanical properties. To overcome these problems, this research aims to: (1) determine the effect of filament dryer modification on the drying process of 3D printing filament, and (2) determine the effect of accelerated drying to shorten the heating time on the mechanical properties of 3D printing filament. Thus, the drying process of materials is important. Therefore, the filament dryer was modified by adding control elements to improve the performance of the 3D printing filament drying process. The drying process reduces the water content trapped inside the 3D-printed filament. The strength of the filament left exposed for 5 weeks was restored through experimental testing by drying at different temperatures and durations. Mechanical properties increased by 1.10% with the combination of 45°C temperature and 6 hours duration, resulting in higher mechanical properties (yield strength) compared to the first time the filament was opened, which exhibited mechanical properties up to 81.4 MPa in the bending test and 41.8 MPa in the tensile test.
Anti-inflammatory response of Metformin Nanoparticles and/or low doses γ-Irradiation on Acute Pancreatitis in Rats via regulation of inflammatory cytokine
Authors: Osman Neamat Ahmed , Mosleh Mohamed , Zahran Faten 
Published: January 2025

Abstract

Repeated pancreatic insult inflammation leads to the development of pancreatitis. In these studies the evaluation of nano-formulated metformin drug with chitosan (Cs-Met NPs) and/or low-doses of γ-irradiation (IR) against acute pancreatitis (AP) induced by L-arginine in an animal model. Thirty adult of Albino rats were divided randomly into 5 groups. Normal control, L-Arginine treated (AP); AP + IR; AP + Cs-Met NPs and AP + IR + Cs-Met NPs. Histopathological studies of pancreatic and biochemical parameters (serum amylase and lipase levels, Random blood glucose (RBS), plasma insulin level, Insulin growth factor 1, tumor necrosis factor-alpha (TNF-α), IL-6, IL-8, IL-10, and CRP were measured in the pancreatitis model. AP induced by L-Arginine- treatment elevates the serum pancreatic amylase & lipase levels, significantly increases IL-6, IL-8, TNF-α, RBS, and CRP, and significantly decreases IL-10, insulin levels, and Insulin growth factor 1. Microscopic examination revealed loss of the pancreatic lobular architecture, marked fibrosis, acinar degeneration, inflammation, and marked oedema. All the serological parameters and the histopathological observations were markedly improved by Cs-Met NPs administration and/or low doses of γ-irradiation treatment. In conclusion, Cs-Met NPs and/or low doses of γ- irradiation have a therapeutic effect on acute pancreatitis model induced in rats.