bluuebunny/crossref_metadata_2025_split huggingface dataset:
The Impact of Eastern Pacific Warming on Future North Atlantic Tropical Cyclogenesis
Authors: Karnauskas Kristopher B. , Heede Ulla K. , Zhang Lei 
Published: September 2023

AbstractTropical cyclogenesis in the Atlantic is influenced by environmental parameters including vertical wind shear, which is sensitive to forcing from the tropical Pacific. Reliable projections of the response of such parameters to radiative forcing are key to understanding the future of hurricanes and coastal risk. One of the least certain aspects of future climate is the warming of the eastern tropical Pacific Ocean. Using climate model experiments isolating the warming of the eastern Pacific and controlling for other factors including El Niño‐Southern Oscillation (ENSO), changes in Atlantic tropical cyclogenesis potential by the end of this century are ∼20% lower with enhanced eastern Pacific warming. The ENSO signal in Atlantic tropical cyclogenesis potential amplifies with global warming, and that amplification is larger with enhanced eastern Pacific warming. The largest changes and dependencies on eastern Pacific warming are found in the south‐central main development region, attributable to changes in zonal overturning.
Exploring a new mechanism between lactate and VSMC calcification: PARP1/POLG/UCP2 signaling pathway and imbalance of mitochondrial homeostasis
Authors: Zhu Yi , Zhang Jia-li , Yan Xue-jiao , Ji Yuan , Wang Fang-fang 
Published: September 2023

AbstractLactate leads to the imbalance of mitochondria homeostasis, which then promotes vascular calcification. PARP1 can upregulate osteogenic genes and accelerate vascular calcification. However, the relationship among lactate, PARP1, and mitochondrial homeostasis is unclear. The present study aimed to explore the new molecular mechanism of lactate to promote VSMC calcification by evaluating PARP1 as a breakthrough molecule. A coculture model of VECs and VSMCs was established, and the model revealed that the glycolysis ability and lactate production of VECs were significantly enhanced after incubation in DOM. Osteogenic marker expression, calcium deposition, and apoptosis in VSMCs were decreased after lactate dehydrogenase A knockdown in VECs. Mechanistically, exogenous lactate increased the overall level of PARP and PARylation in VSMCs. PARP1 knockdown inhibited Drp1-mediated mitochondrial fission and partially restored PINK1/Parkin-mediated mitophagy, thereby reducing mitochondrial oxidative stress. Moreover, lactate induced the translocation of PARP1 from the nucleus to the mitochondria, which then combined with POLG and inhibited POLG-mediated mitochondrial DNA synthesis. This process led to the downregulation of mitochondria-encoded genes, disturbance of mitochondrial respiration, and inhibition of oxidative phosphorylation. The knockdown of PARP1 could partially reverse the damage of mitochondrial gene expression and function caused by lactate. Furthermore, UCP2 was upregulated by the PARP1/POLG signal, and UCP2 knockdown inhibited Drp1-mediated mitochondrial fission and partially recovered PINK1/Parkin-mediated mitophagy. Finally, UCP2 knockdown in VSMCs alleviated DOM-caused VSMC calcification in the coculture model. The study results thus suggest that upregulated PARP1 is involved in the mechanism through which lactate accelerates VSMC calcification partly via POLG/UCP2-caused unbalanced mitochondrial homeostasis.
A twelve-electron conversion iodine cathode enabled by interhalogen chemistry in aqueous solution
Authors: Ma Wenjiao , Liu Tingting , Xu Chen , Lei Chengjun , Jiang Pengjie , He Xin , Liang Xiao 
Published: September 2023

AbstractThe battery chemistry aiming for high energy density calls for the redox couples that embrace multi-electron transfer with high redox potential. Here we report a twelve-electron transfer iodine electrode based on the conversion between iodide and iodate in aqueous electrolyte, which is six times than that of the conventional iodide/iodine redox couple. This is enabled by interhalogen chemistry between iodine (in the electrode) and bromide (in the acidic electrolyte), which provides an electrochemical-chemical loop (the bromide-iodate loop) that accelerates the kinetics and reversibility of the iodide/iodate electrode reaction. In the deliberately designed aqueous electrolyte, the twelve-electron iodine electrode delivers a high specific capacity of 1200 mAh g−1 with good reversibility, corresponding to a high energy density of 1357 Wh kg−1. The proposed iodine electrode is substantially promising for the design of future high energy density aqueous batteries, as validated by the zinc-iodine full battery and the acid-alkaline decoupling battery.
Quaternization-spiro design of chlorine-resistant and high-permeance lithium separation membranes
Authors: Peng Huawen , Yu Kaicheng , Liu Xufei , Li Jiapeng , Hu Xiangguo , Zhao Qiang 
Published: September 2023

AbstractCurrent polyamide lithium extraction nanofiltration membranes are susceptible to chlorine degradation and/or low permeance, two problems that are hard to reconcile. Here we simultaneously circumvented these problems by designing a quaternized-spiro piperazine monomer and translating its beneficial properties into large-area membranes (1 × 2 m2) via interfacial polymerization with trimesoyl chloride. The quaternary ammonium and spiral conformation of the monomer confer more positive charge and free volume to the membrane, leading to one of the highest permeance (~22 L m−2 h−1 bar−1) compared to the state-of-the-art Mg2+/Li+ nanofiltration membranes. Meanwhile, membrane structures are chlorine resistant as the amine–acyl bonding contains no sensitive N-H group. Thus the high performance of membrane is stable versus 400-h immersion in sodium hypochlorite, while control membranes degraded readily. Molecular simulations show that the high permeance and chlorine resistance, which were reproducible at the membrane module level, arise from the spiral conformation and secondary amine structures of the monomer.
Molecular architecture and electron transfer pathway of the Stn family transhydrogenase
Authors: Kumar Anuj , Kremp Florian , Roth Jennifer , Freibert Sven A. , Müller Volker , Schuller Jan M. 
Published: September 2023

AbstractThe challenge of endergonic reduction of NADP+ using NADH is overcome by ferredoxin-dependent transhydrogenases that employ electron bifurcation for electron carrier adjustments in the ancient Wood-Ljungdahl pathway. Recently, an electron-bifurcating transhydrogenase with subunit compositions distinct from the well-characterized Nfn-type transhydrogenase was described: the Stn complex. Here, we present the single-particle cryo-EM structure of the Stn family transhydrogenase from the acetogenic bacterium Sporomusa ovata and functionally dissect its electron transfer pathway. Stn forms a tetramer consisting of functional heterotrimeric StnABC complexes. Our findings demonstrate that the StnAB subunits assume the structural and functional role of a bifurcating module, homologous to the HydBC core of the electron-bifurcating HydABC complex. Moreover, StnC contains a NuoG-like domain and a GltD-like NADPH binding domain that resembles the NfnB subunit of the NfnAB complex. However, in contrast to NfnB, StnC lost the ability to bifurcate electrons. Structural comparison allows us to describe how the same fold on one hand evolved bifurcation activity on its own while on the other hand combined with an associated bifurcating module, exemplifying modular evolution in anaerobic metabolism to produce activities critical for survival at the thermodynamic limit of life.
Highly sensitive and broadband meta-mechanoreceptor via mechanical frequency-division multiplexing
Authors: Li Chong , Liao Xinxin , Peng Zhi-Ke , Meng Guang , He Qingbo 
Published: September 2023

AbstractBio-mechanoreceptors capable of micro-motion sensing have inspired mechanics-guided designs of micro-motion sensors in various fields. However, it remains a major challenge for mechanics-guided designs to simultaneously achieve high sensitivity and broadband sensing due to the nature of resonance effect. By mimicking rat vibrissae, here we report a metamaterial mechanoreceptor (MMR) comprised of piezoelectric resonators with distributed zero effective masses featuring a broad range of local resonances, leading to near-infinite sensitivity for micro-motion sensing within a broad bandwidth. We developed a mechanical frequency-division multiplexing mechanism for MMR, in which the measured micro-motion signal is mechanically modulated in non-overlapping frequency bands and reconstructed by a computational multi-channel demodulation approach. The maximum sensitivity of MMR is improved by two orders of magnitude compared to conventional mechanics-guided mechanoreceptors, and its bandwidth with high sensitivity is extendable towards both low-frequency and high-frequency ranges in 0–12 kHz through tuning the local resonance of each individual sensing cell. The MMR is a promising candidate for highly sensitive and broadband micro-motion sensing that was previously inaccessible for mechanics-guided mechanoreceptors, opening pathways towards spatio-temporal sensing, remote-vibration monitoring and smart-driving assistance.
Heterogeneity in M. tuberculosis β-lactamase inhibition by Sulbactam
Authors: Malla Tek Narsingh , Zielinski Kara , Aldama Luis , Bajt Sasa , Feliz Denisse , Hayes Brendon , Hunter Mark , Kupitz Christopher , Lisova Stella , Knoska Juraj , Martin-Garcia Jose Manuel , Mariani Valerio , Pandey Suraj , Poudyal Ishwor , Sierra Raymond G. , Tolstikova Alexandra , Yefanov Oleksandr , Yoon Chung Hong , Ourmazd Abbas , Fromme Petra , Schwander Peter , Barty Anton , Chapman Henry N. , Stojkovic Emina A. , Batyuk Alexander , Boutet Sébastien , Phillips George N. , Pollack Lois , Schmidt Marius 
Published: September 2023

AbstractFor decades, researchers have elucidated essential enzymatic functions on the atomic length scale by tracing atomic positions in real-time. Our work builds on possibilities unleashed by mix-and-inject serial crystallography (MISC) at X-ray free electron laser facilities. In this approach, enzymatic reactions are triggered by mixing substrate or ligand solutions with enzyme microcrystals. Here, we report in atomic detail (between 2.2 and 2.7 Å resolution) by room-temperature, time-resolved crystallography with millisecond time-resolution (with timepoints between 3 ms and 700 ms) how the Mycobacterium tuberculosis enzyme BlaC is inhibited by sulbactam (SUB). Our results reveal ligand binding heterogeneity, ligand gating, cooperativity, induced fit, and conformational selection all from the same set of MISC data, detailing how SUB approaches the catalytic clefts and binds to the enzyme noncovalently before reacting to a trans-enamine. This was made possible in part by the application of singular value decomposition to the MISC data using a program that remains functional even if unit cell parameters change up to 3 Å during the reaction.
Genomes of cultivated and wild Capsicum species provide insights into pepper domestication and population differentiation
Authors: Liu Feng , Zhao Jiantao , Sun Honghe , Xiong Cheng , Sun Xuepeng , Wang Xin , Wang Zhongyi , Jarret Robert , Wang Jin , Tang Bingqian , Xu Hao , Hu Bowen , Suo Huan , Yang Bozhi , Ou Lijun , Li Xuefeng , Zhou Shudong , Yang Sha , Liu Zhoubing , Yuan Fang , Pei Zhenming , Ma Yanqing , Dai Xiongze , Wu Shan , Fei Zhangjun , Zou Xuexiao 
Published: September 2023

AbstractPepper (Capsicum spp.) is one of the earliest cultivated crops and includes five domesticated species, C. annuum var. annuum, C. chinense, C. frutescens, C. baccatum var. pendulum and C. pubescens. Here, we report a pepper graph pan-genome and a genome variation map of 500 accessions from the five domesticated Capsicum species and close wild relatives. We identify highly differentiated genomic regions among the domesticated peppers that underlie their natural variations in flowering time, characteristic flavors, and unique resistances to biotic and abiotic stresses. Domestication sweeps detected in C. annuum var. annuum and C. baccatum var. pendulum are mostly different, and the common domestication traits, including fruit size, shape and pungency, are achieved mainly through the selection of distinct genomic regions between these two cultivated species. Introgressions from C. baccatum into C. chinense and C. frutescens are detected, including those providing genetic sources for various biotic and abiotic stress tolerances.
The master energy homeostasis regulator PGC-1α exhibits an mRNA nuclear export function
Authors: Mihaylov Simeon R. , Castelli Lydia M. , Lin Ya-Hui , Gül Aytac , Soni Nikita , Hastings Christopher , Flynn Helen R. , Păun Oana , Dickman Mark J. , Snijders Ambrosius P. , Goldstone Robert , Bandmann Oliver , Shelkovnikova Tatyana A. , Mortiboys Heather , Ultanir Sila K. , Hautbergue Guillaume M. 
Published: September 2023

AbstractPGC-1α plays a central role in maintaining mitochondrial and energy metabolism homeostasis, linking external stimuli to transcriptional co-activation of genes involved in adaptive and age-related pathways. The carboxyl-terminus encodes a serine/arginine-rich (RS) region and an RNA recognition motif, however the RNA-processing function(s) were poorly investigated over the past 20 years. Here, we show that the RS domain of human PGC-1α directly interacts with RNA and the nuclear RNA export receptor NXF1. Inducible depletion of PGC-1α and expression of RNAi-resistant RS-deleted PGC-1α further demonstrate that its RNA/NXF1-binding activity is required for the nuclear export of some canonical mitochondrial-related mRNAs and mitochondrial homeostasis. Genome-wide investigations reveal that the nuclear export function is not strictly linked to promoter-binding, identifying in turn novel regulatory targets of PGC-1α in non-homologous end-joining and nucleocytoplasmic transport. These findings provide new directions to further elucidate the roles of PGC-1α in gene expression, metabolic disorders, aging and neurodegeneration.
Machine learning-based anomaly detection of groundwater microdynamics: case study of Chengdu, China
Authors: Shi Haoxin , Guo Jian , Deng Yuandong , Qin Zixuan 
Published: September 2023

AbstractDetection of subsurface hydrodynamic anomalies plays a significant role in groundwater resource management and environmental monitoring. In this paper, based on data from the groundwater level, atmospheric pressure, and precipitation in the Chengdu area of China, a method for detecting outliers considering the factors affecting groundwater levels is proposed. By analyzing the factors affecting groundwater levels in the monitoring site and eliminating them, simplified groundwater data is obtained. Applying sl-Pauta (self-learning-based Pauta), iForest (Isolated Forest), OCSVM (One-Class SVM), and KNN to synthetic data with known outliers, testing and evaluating the effectiveness of 4 technologies. Finally, the four methods are applied to the detection of outliers in simplified groundwater levels. The results show that in the detection of outliers in synthesized data, the OCSVM method has the best detection performance, with a precision rate of 88.89%, a recall rate of 91.43%, an F1 score of 90.14%, and an AUC value of 95.66%. In the detection of outliers in simplified groundwater levels, a qualitative analysis of the displacement data within the field of view indicates that the outlier detection performance of iForest and OCSVM is better than that of KNN. The proposed method for considering the factors affecting groundwater levels can improve the efficiency and accuracy of detecting outliers in groundwater level data.
A systematic review and meta-analysis of the SIRT1 response to exercise
Authors: Juan Ciara Gallardo , Matchett Kyle B. , Davison Gareth W. 
Published: September 2023

AbstractSirtuin 1 (SIRT1) is a key physiological regulator of metabolism and a target of therapeutic interventions for cardiometabolic and ageing-related disorders. Determining the factors and possible mechanisms of acute and adaptive SIRT1 response to exercise is essential for optimising exercise interventions aligned to the prevention and onset of disease. Exercise-induced SIRT1 upregulation has been reported in animals, but, to date, data in humans have been inconsistent. This exploratory systematic review and meta-analysis aims to assess various exercise interventions measuring SIRT1 in healthy participants. A total of 34 studies were included in the meta-analysis (13 single bout exercise, 21 training interventions). Studies were grouped according to tissue sample type (blood, muscle), biomarkers (gene expression, protein content, enzyme level, enzyme activity), and exercise protocols. A single bout of high-intensity or fasted exercise per se increases skeletal muscle SIRT1 gene expression as measured by qPCR or RT-PCR, while repeated resistance training alone increases blood SIRT1 levels measured by ELISA. A limited number of studies also show a propensity for an increase in muscle SIRT1 activity as measured by fluorometric or sirtuin activity assay. In conclusion, exercise acutely upregulates muscle SIRT1 gene expression and chronically increases SIRT1 blood enzyme levels.
Spontaneous degradation of methylene blue adsorbed on magnetic biochars
Authors: Zubrik Anton , Jáger Dávid , Mačingová Eva , Matik Marek , Hredzák Slavomír 
Published: September 2023

AbstractMethylene blue (MB) is one of the most widely studied organic molecules in the treatment of wastewater. Sorption, biodegradation, photodegradation, electrochemical oxidation, ozonation, and other advanced oxidative processes are frequently used to remove this dye from water solutions. The unexpected degradation of MB adsorbed on magnetic biochar from aqueous solution was observed. We found that the conditions of handling, such as drying temperature and storage period, substantially influenced the stability of the dye fixed on the (magnetic) carbon adsorbents. Twelve substances were identified by mass spectrometry as products of decomposition, mostly demethylated, oxidated, and hydroxylated substances. The decomposition of MB was further investigated using non-magnetic carbon biochars and aluminosilicate mineral zeolite. Our findings of the spontaneous decomposition of MB and the identification of the species of degradation offer a new approach to evaluate the mechanism of adsorption, the process of regeneration, and the toxicity of treated solutions.
Integrated system architecture with mixed-reality user interface for virtual-physical hybrid swarm simulations
Authors: Zheng Chuanqi , Jarecki Annalisa , Lee Kiju 
Published: September 2023

AbstractThis paper introduces a hybrid robotic swarm system architecture that combines virtual and physical components and enables human–swarm interaction through mixed reality (MR) devices. The system comprises three main modules: (1) the virtual module, which simulates robotic agents, (2) the physical module, consisting of real robotic agents, and (3) the user interface (UI) module. To facilitate communication between the modules, the UI module connects with the virtual module using Photon Network and with the physical module through the Robot Operating System (ROS) bridge. Additionally, the virtual and physical modules communicate via the ROS bridge. The virtual and physical agents form a hybrid swarm by integrating these three modules. The human–swarm interface based on MR technology enables one or multiple human users to interact with the swarm in various ways. Users can create and assign tasks, monitor real-time swarm status and activities, or control and interact with specific robotic agents. To validate the system-level integration and embedded swarm functions, two experimental demonstrations were conducted: (a) two users playing planner and observer roles, assigning five tasks for the swarm to allocate the tasks autonomously and execute them, and (b) a single user interacting with the hybrid swarm consisting of two physical agents and 170 virtual agents by creating and assigning a task list and then controlling one of the physical robots to complete a target identification mission.
Fabrication and errors in the bibliographic citations generated by ChatGPT
Authors: Walters William H. , Wilder Esther Isabelle 
Published: September 2023

AbstractAlthough chatbots such as ChatGPT can facilitate cost-effective text generation and editing, factually incorrect responses (hallucinations) limit their utility. This study evaluates one particular type of hallucination: fabricated bibliographic citations that do not represent actual scholarly works. We used ChatGPT-3.5 and ChatGPT-4 to produce short literature reviews on 42 multidisciplinary topics, compiling data on the 636 bibliographic citations (references) found in the 84 papers. We then searched multiple databases and websites to determine the prevalence of fabricated citations, to identify errors in the citations to non-fabricated papers, and to evaluate adherence to APA citation format. Within this set of documents, 55% of the GPT-3.5 citations but just 18% of the GPT-4 citations are fabricated. Likewise, 43% of the real (non-fabricated) GPT-3.5 citations but just 24% of the real GPT-4 citations include substantive citation errors. Although GPT-4 is a major improvement over GPT-3.5, problems remain.
Feasibility of aquaculture cultivation of elkhorn sea moss (Kappaphycus alvarezii) in a horizontal long line in the Tropical Eastern Pacific
Authors: Montúfar-Romero Milton , Rincones-León Raúl E. , Cáceres-Farias Lorena Belén , Espinoza-Vera María Mercedes , Avendaño Ulises , Cruz-Jaime Teodoro , Cubillos Luis , Ruiz Walter , Revelo Willan , Lodeiros César , Alfaro-Núñez Alonzo , Cáceres-Farias Lenin 
Published: September 2023

AbstractSeaweed aquaculture has become a profitable and an attractive alternative of cultivation thanks to its quick biomass production for food, feed, and other non-food applications. In addition, the ecosystem services generated by seaweed cultivation towards carbon fixation represents a more sustainable solution to the ocean’s acidification. The growth of elkhorn sea moss (Kappaphycus alvarezii) was evaluated in three plots with 200 propagules during a period of 70 days in a floating raft system covered by a fishing net underneath. Initial weight of propagules was 159.3 ± 12.74 g in wet biomass and 15.3 ± 1.43 g in dry biomass and were sampled up to 19 days (in the lag growth phase; period I), up to 33 days (in the exponential growth phase; period II) and up to 70 days (in the stationarity growth phase; period III). The variations of sea surface water temperature, salinity, turbidity (Secchi depth), total ammonium, nitrites, nitrates, and phosphate were determined. The growth increase was more evident in the exponential phase II when a dry biomass of 28.0 ± 2.48 (1153.3 ± 6.25 g in wet mass) was reached, more than 7 times the biomass of propagules with an average daily growth rate of 15.2% g.day–1. The carrying capacity of the zone was estimated at 86.2% in the area where 53 cultivation units would be projected. The economic analysis presented a financial feasibility with a net profit of 19% over the projected income and an IRR of 16.5%, recovering the investment in an estimated period of 4.3 years. We recommend to continue with larger-scale studies to optimize the cultivation of K. alvarezii in the study area.