bluuebunny/crossref_metadata_2025_split huggingface dataset:
ROS Modulate Cell Death Mechanism in Cervical Cancer Cells Treated
with the Combination of Polyphenolic Compounds and Anticancer Drug
Cisplatin: A Review
Authors: Abdullah Hasmah , Sazeli Syahirah , Mamat Norlida , Hapidin Hermizi , Sulong Sarina 
Published: April 2023

Background:
Most cervical cancer fatalities have been reported due to drug resistance, invasion,
and metastasis. Combination therapy is a prominent technique for overcoming the toxicity of cancer
chemotherapy to normal cells, which is mediated across numerous targeted pathways and requires a
lower dose of each individual agent. Polyphenolic substances have the potential to improve chemotherapy
efficacy while also reducing negative effects.

Aims:
This study aimed to review the research findings on the role of reactive oxygen species (ROS) in
cervical cancer cell HeLa treated with combination therapy.

Results:
Hydroxyl radicals damage DNA, causing a cascade of structural changes in purine and pyrimidine
bases that could lead to mutagenicity. ROS, such as hydroxyl radical (OH-), superoxide anions (O2
-),
hydrogen peroxide (H2O2), and peroxyl radicals (ROO-), are frequent products of aerobic metabolism
that can be beneficial or detrimental to the biological system. To combat the harmful effects of ROS,
cells have an antioxidative defense system that comprises superoxide dismutases, catalase, glutathione,
and other defensive mechanisms. Excessive ROS accumulation causes DNA damage, which triggers
the apoptotic machinery, resulting in cell death.

Conclusion:
Chemotherapeutic medications with phenolic compounds or polyphenol-rich extracts
exhibit anticancer synergy. Combination treatment with polyphenols and anticancer drugs is one of the
promising approaches in the treatment of cervical cancer.
Differential Expression of Down-regulated MicroRNA-126 and Micro
RNA-22 in Ovarian Carcinogenesis
Authors: Paliwal Nidhi , Vashist Minakshi , Chauhan Meenakshi , Yadav Geetanjali , Deshwal Suman , Siwach Kiran 
Published: April 2023

Background:
Non-coding RNA i.e. MicroRNA plays an important role in the progression
and pathogenesis of different types of tumours including ovarian cancer. Deregulated microRNA expression could transform the passivity of cancer, disease progression, chemoresistance, and mobility.
The miRNA profile of healthy and cancerous samples was observed to be different. MicroRNA expression analysis and profiling have a bright future in improving the effectiveness of ovarian cancer
treatment that is customized to the patient's specific requirements. Expression analysis of serum microRNA-126 and microRNA-22 in ovarian carcinogenesis has been done in the present study.

Methods:
Present study has been done in the serum of eighty- two epithelial ovarian cancer patients
and eighty- two their age-matched healthy women to investigate +the expression of miR-126 and miR22 was TaqMan PCR microRNA assay. The correlation of expression miR-126 and miR-22 with epidemiological and clinicopathological factors was determined statistically.

Results:
Results showed a 3.37-fold decrease in miR-126 expression and a 2.67-fold reduction in
miR-22 expression in ovarian cancer patients. Decreased serum level of miR-22 and miR-126 in ovarian cancer patients has been correlated with the FIGO stage. Results revealed 87.46 sensitivity and
91.12 specificities for down-regulated microRNA 22 with FIGO stage of ovarian cancer and 71.26
sensitivity and 81.12 specificities respectively. More sensitivity and specificity of microRNA 22 with
FIGO stage of ovarian cancer was revealed as compared to microRNA 126.

Conclusion:
Present study revealed downregulated expression of miR-126 and miR-22 in the serum
of epithelial ovarian cancer patients. Among all epidemiological and clinical parameters, the FIGO
stage of ovarian cancer revealed a substantial association with microRNA level. Results revealed that
the sensitivity and specificity of down-regulated microRNA 22 in ovarian cancer were 87.46 and
91.12 and for microRNA 126 with 71.26 and 81.12 respectively. This may be investigated and verified as a durable diagnostic biomarker for the pre-diagnosis of ovarian carcinogenesis.
Endometrial Adenofibroma in a Patient Receiving Toremifene: A Case Report
Authors: Li Tao , Li Lu , Liu Gang , Huang Li-Zhao 
Published: April 2023

Introduction:
Adenofibroma is a rare benign Müllerian mixed tumor composed of epithelial and mesenchymal cells. This tumor may occasionally be associated with toremifene therapy which is used as an adjuvant drug for breast cancer.

Case Presentation:
We describe a case of a 55-year-old woman with adenofibroma of the endometrium. This patient was receiving toremifene after surgery and neoadjuvant chemotherapy for breast cancer. She underwent a total abdominal hysterectomy and bilateral salpingectomy. There was no evidence of tumor residual or recurrence at 32 months of MRI follow-up.

Conclusion:
In conclusion, we report a rare case of endometrial adenofibroma in a patient receiving toremifene. It must be borne in mind that long-term toremifene therapy may increase the frequency of endometrial neoplasms.
Experimental and Theoretical Study of Biosurfactants Functionalized
Gold Nanoparticles for Mixture Detection and Chiral Recognition of
Tryptophan by UV-VIS Spectroscopy
Authors: Wu Xiangzong , Li Yanxia , Chen Yiting , Qiu Zhenli , Huang Lu 
Published: April 2023

Background:
Tryptophan (Trp) is an essential amino acid and plays important roles in biological
processes. The detection of Trp is very important for its biological and chemical study. Moreover,
Trp is a chiral compound; due to its importance in biological processes, researchers have been
long committed to the chiral recognition and sensing of Trp enantiomers.

Methods:
Two biosurfactants, sodium cholate and sodium deoxycholate, were used for the preparation
of functionalized gold nanoparticles (AuNPs) which were characterized by transmission electron
microscope and potentiometer. UV-Vis spectra of functionalized gold nanoparticle solutions with different
concentrations of Trp, tyrosine, phenylalanine, D-Trp, and L-Trp were analyzed. Then, the discrimination
mechanism was further investigated, and the promotion mechanism of biosurfactants was
studied by density functional theory (DFT).

Results:
Trp could induce the aggregation of unmodified AuNPs in 2 h, while phenylalanine and tyrosine
could not. Adding biosurfactants promoted the aggregation process, and D- Trp rather than LTrp
was found to be responsible for the aggregation. Therefore, there were interaction differences not
only between Trp, phenylalanine, and tyrosine but also between Trp enantiomers.

Conclusions:
UV-vis spectroscopy could be applied for the direct detection of Trp in mixtures as well
as the chiral recognition of Trp enantiomers. DFT calculations proved that the interactions of D-Trp
with biosurfactants were the strongest, which contributes to the promotion of aggregation.
An Overview and Membership Process of PIC/S and ICH and its
Requirement for Global Regulatory Harmonization of Drugs
Authors: Mahajan Purvika , Thakkar Arti 
Published: April 2023

Abstract:
Due to the increased globalization of regulatory authorities, it has become important to enhance harmonization efforts in creating regulatory requirements, checking and determining GMP
compliance, licensing production locations, recalling defective batches, and boosting information interchange. ICH and PIC/S are the international platforms that provide an appealing and stable framework for addressing such globalization concerns. A country having a membership of PIC/S or ICH
gives an assurance of the quality, safety, and efficacy of their medical products exported across the
globe. Joining such international platforms helps the Pharmaceutical Industries and Regulatory System
of that country to improve quality standards. In the present study, the main objective is to gain
knowledge regarding the procedures to obtain membership in international platforms such as ICH and
PIC/S, and to know the changes/modifications that needs to be done at the institutional/organizational
level before applying for the membership, also, to gain knowledge of the eligibility criteria for the application and to know the differences in regulatory guidance in GMP aspects of different regulatory
authorities. By joining these international platforms, ICH and PIC/S, non-member countries will be
able to tap into the potential for pharmaceutical exports, especially in the largest countries of the
globe. After joining these international platforms, the semi or low-regulated nations’ pharmaceutical
industries will be benefited greatly in terms of cost savings, export convenience, and most importantly, market access. Membership in ICH and PIC/S will be able to provide the semi or low-regulated
Drug Regulatory Authority a global footprint and give foreign regulatory agencies confidence in semi
or low-regulated regulatory agencies.
Study on Migration Characteristics of Pollutants in Groundwater at a
Proposed Hazardous Waste Landfill
Authors: Fa-you A. , Wang Rui , Dai Xue-Gang , Wu Wen-jie , Yan Shi-qun 
Published: April 2023

Objective:
The paper aims to analyze the hydrogeological conditions of a proposed
hazardous waste landfill and the migration characteristics of lead, zinc, and nickel in fractured
aquifers and porous aquifers under accident conditions and provide a reference for the influence
of the proposed landfill on groundwater.

Methods:
In this study, based on a 1:50000 regional hydrogeological survey and 1:2000 site
hydrogeological mapping, the hydrogeological conceptual model was established. Finite difference
software GMS was used to analyze the migration characteristics.

Results:
The study demonstrated that when the pollutants in the hazardous waste landfill leaked,
they migrated from northeast to southwest along the gully. The pollutants in the porous aquifer
migrated quickly, and the polluted area expanded rapidly from point to surface. The pollutants
migration in fractured aquifers was slow, and the groundwater quality was deteriorating continuously.
During the simulation period, the pollutants of lead, zinc and nickel all polluted the aquifer.
Among them, the lead pollution range w reported to be the largest, with an exceeding distance
of 216.7 m; the zinc pollution range was the smallest, with an exceeding distance of 33.3
m, and the exceeding distance of nickel was 165.1 m.

Conclusion:
In order to ensure the safety of the groundwater environment in the simulated area,
the impervious treatment must be carried out according to the requirements of the proposed hazardous
waste landfill. Meantime, an emergency plan should be formulated.
A Decenary Update on Metal Oxide Nanoparticles as a Heterogeneous
Greener Catalyst for the Synthesis of Bioactive Heterocycles
Authors: Kamanna Kantharaju , Badiger Krishnappa B 
Published: April 2023

Abstract:
In recent years, heterocycle derivatives have emerged as promising molecules, and have exhibited remarkable pharmacological applications. The statistical data analysis of the presently available drug molecules in the market has revealed that more than 70% of the drug candidates are derived from the heterocycles. Various synthetic protocols have been established employing a wide range of catalysts and reaction conditions; among them, one of the catalytic areas includes nanomaterials of metals and metal oxides. Nanocatalysts play an important role in the organic transformation under green chemistry protocol, due to their recycling nature and provision of the required catalytic amount. In this review, we have provided a comprehensive summary of the re-cent progress made in the catalytic heterogeneous metal oxide NPs application, exclusively for the synthesis of heterocyclic compounds reported in the period from 2012 to 2021. Also, this review provides an inherent framework for the reader to select a suitable catalytic system of interest to synthesize desired oxygen, nitrogen, and sulphur heteroatoms containing heterocyclic scaffold with potential pharmacological activities.
Heat Therapy: Concept, Mechanism and Application in Unani Medicine
Authors: Amanullah  , Shamim  , Sayeed Ahmad , Haque Merajul , Ali  Khan Asim 
Published: April 2023

Background:
Application of heat either generalised or localised is among various means employed in Unani medicine for promoting health, preventing as well as treating diseases. The concept of hotness (Ḥarārat) and application of physical heat have been in vogue during different periods of human civilization and it exists even today with certain modifications.

Heating Modalities:
Unani medicine offers various methods for the application of mild to moderate heat that includes Turkish bath (Ḥammām), massage (Dalk), cupping (Ḥijāma), sitz bath (Ābzan), inducing diaphoresis (Ta‘rīq), douche (Naṭūl), vapour bath (Inkibāb) and footbath (Pāshoya).

Application of Heat:
Heating modalities are commonly used by Unani physicians for relieving pain and stiffness, relaxing muscles, increasing blood circulation, liquefying the sticky and adherent humours lodged in the peripheral tissues and reducing inflammation. Application of severe heat also finds a place among various modes of treatment mentioned in Unani medicine. Cauterisation (Kayy) is the mode that is employed for the stoppage of bleeding, removing putrefactive matter and destroying lesions.

Conclusion:
Unani medicine offers classical methods and instruments for heat therapy used for centuries for the promotion of health, prevention and management of diseases. Heat therapy has been found considerably effective in managing certain health problems and diseases.
An Overview of Recently Published Patents on Pulmonary Drug Delivery
Devices
Authors: Dongare Suraj L. , Narkhede Minal R. 
Published: April 2023

Abstract:
The pulmonary drug delivery system is a minimally invasive method of administering drugs
with systemic and localised activity. Since 4000 BC, inhalation therapy has been known to the Indians.
The most effective and suitable pulmonary drug delivery methods have been used for controlling diseases
like asthma, chronic obstructive pulmonary disorder (COPD), TB(Tuberculosis), lung cancer, cystic
fibrosis, and pulmonary hypertension. Examples of pulmonary medication delivery devices- Metered
dose inhalers (MDIs), nebulizers, and dry powder inhalers (DPIs) in the latest patent 2022 that have undergone
numerous advancements over the years have been focused on in this article. Some promising patented
design advancements of nebulizers are humidifier breathing circuits to control liquid contamination,
technologically advanced nebulizers to increase pressure detection and nebulizer disinfection system
to decrease or eliminate contagions in expelled air are highlighted in this article. Some noticed formulation-
related advancements for inhalational dry powder patented in the year 2022, are mentioned in this
article. Development of heat-stable dry powder to solve instability of inhaled protein and peptide powder
at high temperatures. The inability of dry powder inhaler devices to administer low doses is solved by designing
an affordable and side effects-free inhaler. pMDI manufacturing process is simplified by manufacturing
tablets to be administered in pMDI. An aid is developed to lessen the activation force and keep
the dose count within budget. The patented advancement in the pulmonary drug delivery system can help
in the improvement of patient compliance and drug delivery efficacy.
A IMPORTÂNCIA DA INSTALAÇÃO DE UM PROTOCOLO DE HIGIENE ORAL EM PACIENTES ENTUBADOS REVISÃO DE LITERATURA
Authors: Clementino Cordeiro Luana , Rocha Vasconcellos Ribeiro Jade , Azevedo Goulart Amaral Aramis , Tristão Werneck Juliana 
Published: April 2023

A instalação de ventilação mecânica permite a manutenção da vida no processo de recuperação de pacientes hospitalizados que necessitam de auxílio respiratório. No entanto, a presença do ventilador no meio bucal propicia o acúmulo de debris e a retenção de placa. A cavidade oral é um meio rico em microrganismos e, em casos de pacientes entubados, o biofilme bucal pode abrigar patógenos respiratórios que levam ao desenvolvimento da Pneumonia Associada a Ventiladores (PAV). Essa infecção é a mais comum das infecções secundárias desenvolvidas em pacientes entubados e aumenta as chances de morbidade e mortalidade, bem como prolonga a hospitalização e seus custos. O objetivo desta revisão da literatura foi avaliar o impacto da higiene bucal na prevenção de PAV em pacientes entubados. Utilizando os descritores "Oral care", "Intubated Patients“ e “Cross Infection” na base de dados PubMed, foram encontrados 143 artigos. Destes, foram selecionados os estudos gratuitos publicados entre 2015 e 2020, que envolviam pesquisa em humanos adultos e jovens adultos. Foram descartados aqueles em que os pacientes em CTI e UTI não estavam entubados, restando 12 artigos para serem lidos integralmente. Segundo a literatura, a higienização da cavidade oral se mostra eficaz na redução de incidência da PAV, sendo o método mais utilizado a limpeza química com Clorexidina em concentrações variando de 0,12% a 2%, podendo ser complementada com a higienização mecânica. Assim, a implementação de um protocolo de higiene bucal para pacientes entubados é de fundamental importância para diminuir as chances de desenvolvimento de PAV.
Palavras Chave: Ventilação mecânica, Pneumonia Associada ao Ventilador, higiene bucal
ADVANCES IN SURFACE TREATMENT OF DENTAL IMPLANTS: A LITERATURE REVIEW
Authors: Ferreira Leticia Gonçalves , Salles Tamyres Silva , Câmara Eduardo Aguiar , Pesce Giovanna , Resende Rodrigo Figueiredo De Brito , Uzeda Marcelo José Pinheiro Guedes De , Calasans-Maia Mônica Diuana , Sartoretto Suelen Cristina 
Published: April 2023

Amplamente utilizados para substituir dentes perdidos, os implantes dentários nos últimos anos vêm apresentando tecnologias como superfícies com micro e nanotopografia e ajustes nas composições químicas, dentre outros, para melhorar a osseointegração e reduzir o prazo de tratamento, permitindo, assim, carga funcional imediata ou precoce em pacientes com densidade óssea reduzida. Vários métodos são aplicados com intuito de modificar a superfície do implante, como jateamento com areia, corrosão ácida, oxidação anódica, tratamento com flúor, usinagem, pulverização de plasma de titânio e revestimento de fosfato de cálcio; esses métodos podem aumentar notavelmente a área de superfície quando operada a técnica adequadada de modificação, quer por procedimento de adição ou subtração. Tais modificações promovem superfícies rugosas, as quais aumentam a porcentagem de contato osso-implante (BIC)durante o processo de cicatrização óssea inicial. Os principais benefícios da modificação da superfície são melhorar a molhabilidade (hidrofilicidade), adesão e fixação de células a implantes e proliferação celular. Dentre os tratamentos de superfície de implantes dentários destaca-se o jateamento de areia com granulação grossa e ataque-ácido com HCL/H2SO4 (SLA) em altas temperaturas, o revestimento de superfície do implante com hidroxiapatita, oxidação anódica e o duplo ataque ácido. O objetivo deste trabalho é realizar uma revisão de literatura discutindo a importância e eficácia desses métodos para a osseointegração e, por conseguinte, para a redução do período de tratamento.
Palavras-chave: Osseointegração; Implantes dentários; Superfície; Modificação
ANÁLISE ENDOSCÓPICA DAS ALTERAÇÕES NA ELEVAÇÃO DO ASSOALHO DO SEIO MAXILAR COM ABORDAGEM DA TÉCNICA DE SUMMER E IMPLANTE IMEDIATO: UM ENSAIO CLÍNICO COM 10 ANOS DE ACOMPANHAMENTO
Authors: Harduin-Couto Marcelo , Gonçalves Alexandre Oliveira , Rizzato Marcelo , Oliveira Evandro Ribeiro de , Fischer-Trindade Hiram , Fernandes Gustavo Vicentis de Oliveira 
Published: April 2023

O objetivo deste estudo foi avaliar através de endoscopia as possíveis intercorrências durante levantamento de seio maxilar fechado, além de verificar a taxa de sobrevivência dos implantes após 10 anos. Seis pacientes (12 seios maxilares) foram incluídos neste estudo com idades entre 26 e 74 anos. O mesmo cirurgião realizou os procedimentos, sob anestesia local e sedação venosa em ambiente hospitalar. Todos os participantes foram submetidos à elevação do seio nasal e colocação do implante em apenas um procedimento, conforme planejado anteriormente, por acompanhamento transcirúrgico da endoscopia (Stortz®). Todos os casos receberam enxerto ósseo bovino (Bio-Oss®) antes da colocação do implante. Após 10 anos, os pacientes foram chamados para acompanhamento. Foram detectadas duas intercorrências (16,66%) com o endoscópio, uma ruptura simples e outra perfuração com extravasamento do enxerto para dentro do seio. Ambos foram revertidos e corrigidos imediatamente. Houve perda de um implante (8,33%), portanto esse paciente não apresentou intercorrência no transoperatório e a membrana estava elevada menos de 5 mm. A taxa de sobrevivência alcançada foi de 91,66%. A técnica do osteótomo constitui um método confiáel em longo prazo (10 anos) apresentando uma alta taxa de sobrevivência do implante, sugerindo que uma elevação de até 5,5 mm em pacientes saudáveis é possível. Assim, verificou-se que as ocorrências no transoperatório foram detectadas apenas pela análise endoscópica que deve ser estimulada para garantir uma visibilidade mais segura. Por outro lado, a associação da técnica fechada com o endoscópio foi difícil, aumentou os custos, limitando seu uso como rotina.
Palavras-chave: Endoscopia; Seio maxilar; Elevação de seio maxilar; Técnica de Summers; Técnica fechada; Avaliação em longo prazo.
RETENÇÃO DO CONHECIMENTO SOBRE SAÚDE BUCAL EM UMA AMOSTRA DE ESCOLARES DE NOVA FRIBURGO.
Authors: Cordovil Siqueira Rhayanne , Mikhael Ammari Michelle , Thomas Canabarro de Andrade Marcia 
Published: April 2023

As medidas de orientação para a prevenção da cárie apoiam-se na educação em saúde bucal do paciente. Este trabalho avaliou a retenção do conhecimento sobre saúde bucal em escolares, antes e depois da realização de oficinas educativas. Após a aprovação no comitê de ética em pesquisa (CEP Nº2.987.965) este estudo foi realizado com crianças do ensino fundamental de uma escola pública em Nova Friburgo-RJ. Um questionário sobre práticas de higiene bucal e dieta, e conhecimento sobre as doenças bucais foi desenvolvido e aplicado antes da realização de oficinas educativas em saúde bucal e 15, 30, 60 e 90 dias após. A amostra foi de 136 escolares, 54,4% meninos e 45,6% meninas, com média de idade de 8,7 anos. Antes das oficinas as crianças relataram limpar seus dentes todos os dias (91,2%), pelo menos três vezes ao dia (60,3%), utilizando escova, pasta e fio dental (52,9%); 59,6% das crianças relataram saber o que causava a cárie antes das oficinas; 15 e 30 dias após as oficinas, os percentuais foram de 94,9% e 98,5%, respectivamente (p<0,05), diminuindo para 58,8% após 60 dias. Para a gengivite, apenas 11,8% das crianças relataram saber a causa da doença antes das oficinas; 15 e 30 dias após os percentuais foram de 81,6% e 94,9%, respectivamente, diminuindo para 13,2% após 60 dias. Com base nos resultados observou-se uma diminuição na retenção do conhecimento após 30 dias da realização das atividades educativas, sugerindo que esta população deva receber um programa de educação em saúde bucal de forma continuada.

Palavras chaves: Cárie dentária, Saúde bucal, Educação em saúde.