bluuebunny/crossref_metadata_2025_split huggingface dataset:
Experimental study on the pull‐down performance of a −80°C cascade refrigeration freezer
Authors: Tan Haihui , Bai Minkai , Xu Lingfei , Li Xiong , Liu Zhan 
Published: February 2023

AbstractTo cope with global climate change, chlorofluorocarbons and hydrofluorocarbons refrigerants have been or will be abandoned by different countries worldwide. Natural refrigerants seem to be promising substitutes in the near future. In this study, eco‐friendly refrigerants R290 and R170 are adopted as high‐temperature and low‐temperature fluid in a cascade refrigeration freezer to generate −80°C. The experimental measurements are carried out to investigate the pull‐down performance of the freezer. The inlet and outlet pressure of two compressors are monitored and tested. Temperatures of different state monitors in high‐temperature cycle and low‐temperature cycle are measured to analysis the operation performance of the cascade refrigeration system. Different temperature test points are set to research the temperature drop and temperature distribution in the freezer. Some new findings are obtained. Due to the lack of ultralow temperature experimental data on cascade refrigeration system, this study can enrich and deepen the understanding on operation mechanism of ultralow temperature cascade refrigeration freezer.
From Cosmopolitan to Vernacular in the Language Sciences: A Global History Perspective
Authors: Leezenberg Michiel 
Published: February 2023

AbstractSheldon Pollock's justly famous work on cosmopolitan orders and processes of vernacularization in the worlds of Latinity and Sanskrit invites questions of a comparative and global‐historical character. I will raise such questions in the context of the Persianate cosmopolitan order, especially as exemplified by the early modern Ottoman Empire, focusing on the wave of vernacularizations this empire witnessed in the seventeenth–eighteenth centuries. In this process of vernacularization, new vernacular forms of philological learning appear to have played a crucial role. Building on Bourdieu's work, I will try to analyze the Ottoman cosmopolitan as a pre‐modern form of linguistic domination, and vernacularization as a form of resistance. Moving beyond Bourdieu, I will be arguing for a genealogical approach that is alive to premodern non‐European philological traditions, and to the historically variable relation between (philological) knowledge and power.
On the role of benchmarking data sets and simulations in method comparison studies
Authors: Friedrich Sarah , Friede Tim 
Published: February 2023

AbstractMethod comparisons are essential to provide recommendations and guidance for applied researchers, who often have to choose from a plethora of available approaches. While many comparisons exist in the literature, these are often not neutral but favor a novel method. Apart from the choice of design and a proper reporting of the findings, there are different approaches concerning the underlying data for such method comparison studies. Most manuscripts on statistical methodology rely on simulation studies and provide a single real‐world data set as an example to motivate and illustrate the methodology investigated. In the context of supervised learning, in contrast, methods are often evaluated using so‐called benchmarking data sets, that is, real‐world data that serve as gold standard in the community. Simulation studies, on the other hand, are much less common in this context. The aim of this paper is to investigate differences and similarities between these approaches, to discuss their advantages and disadvantages, and ultimately to develop new approaches to the evaluation of methods picking the best of both worlds. To this aim, we borrow ideas from different contexts such as mixed methods research and Clinical Scenario Evaluation.
Phytochemical Screening, Antibacterial, Antifungal, Anti‐Biofilm and Antioxidant Activity of Azadiracta Indica A. Juss. Flowers
Authors: Mohanasundaram Palanivelmurugan , Saral A. Mary 
Published: February 2023

AbstractThe present study involves investigation of Azadiracta Indica flowers with respect to its pharmacognostic properties, phytochemical screening, and its application as anti‐oxidant, anti‐biofilm, and anti‐microbial agent. The Pharmacognostic characteristics were evaluated with respect to moisture content, total ash content, acid, and water‐soluble ash content, swelling index, foaming index, and metal content. The macro and micronutrient content of the crude drug was estimated by AAS and Flame photometric methods and it gives the quantitative estimation of minerals, where calcium is present in abundance (88.64 mg/L). Soxhlet extraction was carried out in the increasing order of polarity of the solvent viz Petroleum Ether (PE), Acetone (AC), and Hydroalcohol (20 %) (HA) to extract the bioactive compounds. The characterization of the bioactive compounds of all the three extract have been carried out using gcms and lcms. The presence of 13 major compounds have been identified in PE extract and 8 compounds in AC extract using gcms studies. The HA extract is found to contain polyphenols, flavanoids, and glycosides. The antioxidant activity of the extracts was evaluated by DPPH, FRAP, and Phosphomolybdenum assay. This reveals that HA extract shows good scavenging activity than PE and AC extracts which is well correlated with the bioactive compounds, especially phenols which are present as a major component in the extract. The anti‐microbial activity was investigated via Agar well diffusion method for all the extracts. Among all the extracts HA extract shows good antibacterial activity with MIC of 25 μg/mL and AC extract shows good anti‐fungal activity with MIC of 25 μg/mL. The antibiofilm assay confirms that the HA extract shows good biofilm inhibition about 94 % among other extracts when tested on human pathogens. The results confirm that the HA extract of A. Indica flowers will be an excellent source of natural anti‐oxidant and also antimicrobial agents. This paves the way for its potential uses in herbal product formulation.
Synthesis, Biological Evaluation and in Silico Study of N‐(2‐ and 3‐Pyridinyl)benzamide Derivatives as Quorum Sensing Inhibitors against Pseudomonas aeruginosa
Authors: Sharma Nikhil , Srivastava Namita , Devi Bharti , Kumar Lokender , Kumar Rajnish , Kumar Yadav Ashok 
Published: February 2023

AbstractThe effectiveness of treating bacterial infections is seriously threatened by the emergence of bacterial resistance to chemical treatment. Growth of microbes in biofilm is one of the main causes of resistance to antimicrobial drugs. Quorum sensing (QS) inhibition, which targets the QS signalling system by obstructing cell‐cell communication, was developed as an alternative treatment by creating innovative anti‐biofilm drugs. Therefore, the goal of this study is to develop novel antimicrobial drugs that are effective against Pseudomonas aeruginosa by inhibiting QS and acting as anti‐biofilm agents. In this study, N‐(2‐ and 3‐pyridinyl)benzamide derivatives were selected to design and syntheses. Antibiofilm activity was revealed by all the synthesized compounds and the biofilm was visibly impaired, and the OD595nm readings of solubilized biofilm cells presented a momentous difference between the treated and untreated biofilms. The best anti‐QS zone was observed for compound 5d and found to be 4.96 mm. Through in silico research, the physicochemical characteristics and binding manner of these produced compounds were examined. For the purpose of understanding the stability of the protein and ligand complex, molecular dynamic simulation was also carried out. The overall findings showed that N‐(2‐ and 3‐pyridinyl)benzamide derivatives could be the key to creating effective newer anti‐quorum sensing drugs that are effective against different bacteria.
Neutral, Heteroleptic [Cu(I)(PPh3)2(4H‐imidazolato)] Complexes: Ligand Exchange Reactivity, Redox Properties, Excited‐State Dynamics
Authors: Seidler Bianca , Tran Jens H. , Thomisch Luise , Vashistha Nikita , Görls Helmar , Liebing Phil , Schulz Martin , Dietzek‐Ivanšić Benjamin 
Published: February 2023

AbstractCu(I) 4H‐imidazolate complexes are rare examples of Cu(I) complexes with chelating anionic ligands and are potent photosensitizers with unique absorption and photoredox properties. In this contribution, five novel heteroleptic Cu(I) complexes with monodentate triphenylphosphine co‐ligands are investigated. As a consequence of the anionic 4H‐imidazolate ligand and in contrast to comparable complexes with neutral ligands, these complexes are more stable than their homoleptic bis(4H‐imidazolato)Cu(I) congeners. Here, the ligand exchange reactivity was studied by 31P‐,19F‐, and variable temperature NMR and the ground state structural and electronic properties by X‐ray diffraction, absorption spectroscopy, and cyclic voltammetry. The excited‐state dynamics were investigated by femto‐ and nanosecond transient absorption spectroscopy. The observed differences, with respect to chelating bisphosphine bearing congeners, are often due to the increased geometric flexibility of the triphenylphosphines. These observations render the investigated complexes interesting candidates for photo(redox)reactions not accessible with chelating bisphosphine ligands.
The Manufacture and Characterization of Biomimetic, Biomaterial‐Based Scaffolds for Studying Physicochemical Interactions of Neural Cells in 3D Environments
Authors: O'Connor Cian , Woods Ian , Hibbitts Alan , Dervan Adrian , O'Brien Fergal J. 
Published: February 2023

AbstractA particular challenge to the field of neuroscience involves translating findings from 2D in vitro systems to 3D in vivo environments. Standardized cell culture environments that adequately reflect the properties of the central nervous system (CNS) such as the stiffness, protein composition, and microarchitecture in which to study 3D cell–cell and cell–matrix interactions are generally lacking for in vitro culture systems. In particular, there remains an unmet need for reproducible, low‐cost, high‐throughput, and physiologically relevant environments comprised of tissue‐native matrix proteins for the study of CNS microenvironments in 3D. Advances in the field of biofabrication over the past number of years have facilitated the production and characterization of biomaterial‐based scaffolds. Typically developed for tissue engineering applications, they also provide sophisticated environments in which to study cell–cell and cell–matrix interactions and have been used for 3D modeling for a range of tissues. Here, we describe a simple and scalable protocol for the production of biomimetic, highly porous freeze‐dried hyaluronic acid scaffolds with tunable microarchitecture, stiffness, and protein composition. Furthermore, we describe several different approaches that can be used to characterize a range of physicochemical properties and how to employ the scaffolds for the 3D culture of sensitive CNS cells in vitro. Finally, we detail several approaches for the study of key cell responses within the 3D scaffold environments. Overall, this protocol describes the manufacture and testing of a biomimetic and tunable macroporous scaffold system for neuronal cell culture applications. © 2023 The Authors. Current Protocols published by Wiley Periodicals LLC.Basic Protocol 1: Scaffold manufactureBasic Protocol 2: Scaffold characterizationBasic Protocol 3: Cell culture and analysis of neurons in scaffolds
The role of task difficulty in directing selective attention in bobwhite quail (Colinus virginianus) neonates: A developmental test of the intersensory redundancy hypothesis
Authors: Lickliter Robert , Bahrick Lorraine E. , Vaillant‐Mekras Jimena 
Published: February 2023

AbstractThe dynamics of selective attention necessarily influences the course of early perceptual development. The intersensory redundancy hypothesis proposes that in early development information presented redundantly across two or more senses selectively recruits attention to the amodal properties of an object or event. In contrast, information presented to a single sense enhances attention to modality‐specific properties. The present study assessed the second of these predictions in neonatal bobwhite quail (Colinus virginianus), with a focus on the role of task difficulty in directing selective attention. In Experiment 1, we exposed quail chicks to unimodal auditory, nonredundant audiovisual, or redundant audiovisual presentations of a bobwhite maternal call paired with a pulsing light for 10 min/h on the day following hatching. Chicks were subsequently individually tested 24 h later for their unimodal auditory preference between the familiarized maternal call and the same call with pitch altered by two steps. Chicks from all experimental groups preferred the familiarized maternal call over the altered maternal call. In Experiment 2, we repeated the exposure conditions of Experiment 1, but presented a more difficult task by narrowing the pitch range between the two maternal calls during testing. Chicks in the unimodal auditory and nonredundant audiovisual conditions preferred the familiarized call, whereas chicks in the redundant audiovisual exposure group showed no detection of the pitch change. Our results indicate that early discrimination of pitch change is disrupted by intersensory redundancy under difficult but not easy task conditions. These findings, along with findings from human infants, highlight the role of task difficulty in shifting attentional selectivity and underscore the dynamic nature of neonatal attentional salience hierarchies.
Limited population and community effects of hatching asynchrony in a pond‐breeding salamander
Authors: Anderson Thomas L. , Burkhart Jacob J. , Cianci‐Gaskill Jacob A. , Davenport Jon M. 
Published: February 2023

AbstractUnderstanding attributes of phenology beyond the mean date of a life history event, such as variability among individuals within a population, is critical to predict how climate‐induced phenological shifts may alter population dynamics. Identifying how phenological variability impacts organisms is especially needed to better understand how phenological shifts affect trophic dynamics (e.g., shifts in variability of top predators affecting primary production). To better understand the effects of phenological variability on both populations and communities, we examined how variation in egg hatching synchrony of predatory marbled salamanders (Ambystoma opacum) impacted intraspecific interactions at the larval stage, ultimately affecting demographic traits and survival through metamorphosis. We also examined how hatching synchrony affected overall trophic dynamics (e.g., primary consumers and producers) in pond food webs. We experimentally manipulated the degree of hatching synchrony of embryonic A. opacum and subsequently reared larvae in outdoor mesocosms. We monitored demographic traits such as larval growth, size at and time to metamorphosis, and survival. To assess trophic dynamics, we monitored zooplankton abundance and phytoplankton biomass during the experiment. Larvae exhibited greater variability in body size in medium and low hatching synchrony treatments compared to high synchrony treatments. Larval body size variation diminished over time to ultimately result in no differences in most life history traits at metamorphosis or survival among hatching synchrony treatments. We also found no differences among treatments in zooplankton abundance or phytoplankton biomass, likely because of minimal variation in A. opacum survival among treatments that would induce top‐down changes. Overall, we found that phenological variation may be context dependent in its influence on demography and overall community structure. Because of concerns for how phenological shifts will affect species interactions, greater scrutiny into conditions that would promote changes in population and community dynamics is needed.
Between two trees: Environmental effects of I. micheliana and A. latifolia on leaf litter ants in a coffee agroecosystem
Authors: Aponte Rolón Bolívar , Perfecto Ivette 
Published: February 2023

AbstractShade trees provide important ecological services that support productivity in coffee agroforestry systems. Processes such as biological nitrogen fixation play a key role in this. Less is known, however, about potential indirect mechanisms by which nitrogen fixation supports coffee productivity. One potential route for this to occur is by providing ecological benefits to other above‐ and belowground organisms that enrich the overall function of agroecosystems. A useful lens with which to evaluate the ecological benefits to these communities under shade trees is to assess how ground‐dwelling ant communities respond to the quality of leaf litter from established nitrogen (N)‐fixing tree species. Here, we use two trees commonly planted in coffee agroecosystems: Inga micheliana, an N‐fixing species, and Alchornea latifolia, a non‐N‐fixing species. In this study, we set out to answer the following questions: (1) How does the leaf litter environment differ between I. micheliana and A. latifolia? (2) Do differences in environmental factors between I. micheliana and A. latifolia correlate with differences in ant abundance and species richness? and (3) Do differences in environmental factors between I. micheliana and A. latifolia correlate with differences in ant community composition? Twenty‐eight randomly selected sites (14 I. micheliana and 14 A. latifolia) were established within a 45‐ha plot in a shaded organic coffee farm in Chiapas, Mexico. Three 1‐m2 quadrats within a 5‐m radius from the base of the selected trees were established, and the leaf litter within the quadrats was removed and sieved. Ant specimens were extracted from leaf litter collected from quadrats using the mini‐Winkler method and identified to genus and species, or morphospecies, level. Results indicate that I. micheliana, the N‐fixing species, has a lower C:N ratio than A. latifolia. Differences in C:N ratios are correlated with ant abundance but not with ant species richness. Distance to edge (in meters) has significant effects on leaf litter ant abundance, richness, and species composition. Results suggest that there may be unaccounted feedbacks from N‐ and non‐N‐fixing vegetation to brown food webs enabling them to sustain similar ground‐dwelling ant communities.
Isolated functional tricuspid regurgitation: how to define patients at risk for event?
Authors: L'Official Guillaume , Vely Mathilde , Kosmala Wojciech , Galli Elena , Guerin Anne , Chen Elisabeth , Sportouch Catherine , Dreyfus Julien , Oger Emmanuel , Donal Erwan 
Published: February 2023

AbstractAimsFunctional tricuspid regurgitation (TR) is a turning point in cardiac diseases. Symptoms typically appear late. The optimal timing for proposing a valve repair remains a challenge. We sought to analyse the characteristics of right heart remodelling in patients with significant functional TR to identify the parameters that could be used in a simple prognostic model predicting clinical events.Methods and resultsWe designed a prospective observational French multicentre study including 160 patients with significant functional TR (effective regurgitant orifice area > 30 mm2) and left ventricular ejection fraction > 40%. Clinical, echocardiographic, and electrocardiogram data were collected at baseline and at the 1 and 2 year follow‐up. The primary outcome was all‐cause death or hospitalization for heart failure. At 2 years, 56 patients (35%) achieved the primary outcome. The subset with events showed more advanced right heart remodelling at baseline, but similar TR severity. Right atrial volume index (RAVI) and the tricuspid annular plane systolic excursion to systolic pulmonary arterial pressure (TAPSE/sPAP) ratio, reflecting right ventricular–pulmonary arterial coupling, were 73 mL/m2 and 0.40 vs. 64.7 mL/m2 and 0.50 in the event vs. event‐free groups, respectively (both P < 0.05). None among all the clinical and imaging parameters tested had a significant group × time interaction. The multivariable analysis leads to a model including TAPSE/sPAP ratio > 0.4 (odds ratio = 0.41, 95% confidence limit 0.2 to 0.82) and RAVI > 60 mL/m2 (odds ratio = 2.13, 95% confidence limit 0.96 to 4.75), providing a clinically valid prognostic evaluation.ConclusionsRAVI and TAPSE/sPAP are relevant for predicting the risk for event at 2 year follow‐up in patients with an isolated functional TR.
Memory B‐cell diversity: From early generation to tissue residency and reactivation
Authors: Reusch Laura , Angeletti Davide 
Published: February 2023

AbstractMemory B cells (MBCs) have a crucial function in providing an enhanced response to repeated infections. Upon antigen encounter, MBC can either rapidly differentiate to antibody secreting cells or enter germinal centers (GC) to further diversify and affinity mature. Understanding how and when MBC are formed, where they reside and how they select their fate upon reactivation has profound implications for designing strategies to improve targeted, next‐generation vaccines. Recent studies have crystallized much of our knowledge on MBC but also reported several surprising discoveries and gaps in our current understanding. Here, we review the latest advancements in the field and highlight current unknowns. In particular, we focus on timing and cues leading to MBC generation before and during the GC reaction, discuss how MBC become resident in mucosal tissues, and finally, provide an overview of factors shaping MBC fate‐decision upon reactivation in mucosal and lymphoid tissues.
Development of Macrocyclic Mn(II)−Bispyridine Complexes as pH‐Responsive Magnetic Resonance Imaging Contrast Agents
Authors: Shen Xiaojuan , Pan Yan , Liang Guohai 
Published: February 2023

AbstractIn this study, macrocyclic complexes of Mn (II) with pH‐responsive relaxivities were developed. The ligands were based on the rigid MPi platform, which was composed of a triazacyclononane (TACN) macrocycle and two picolyl pendant arms. An aryl sulfonamide moiety introduced in the skeleton of MPi endowed the final Mn complex (Mn−MPA) with pH‐sensitive relaxivities. Mn−MPA showed a ∼3‐fold increase in longitudinal relaxivity associated with the protonation and decoordination of the sulfonamide group in an acidic environment. We found that it was feasible to tune the pH window of the complexes by choosing proper substituents on the aromatic ring or modifying on the picolyl pendants. Considering the good kinetic inertness, rapid and efficient response to pH stimuli, and easy modulation characteristics, Mn−MPAs can be considered ideal candidates for use as acidic microenvironment‐specific MRI contrast agents.