bluuebunny/crossref_metadata_2025_split huggingface dataset:
Evaluation of the fungicide treatment with copper oxide and potassium phosphonate solutions for the sustainable management of P. pinaster trees infected with B. xylophilus
Authors: López-Villamor Adrián , Silva Marta Nunes da , Vasconcelos Marta W. 
Published: March 2023

Abstract
Fungicides induce changes in the plants promising to increase tolerance of Pinus pinaster against the pathogenic pinewood nematode (PWN). To test this hypothesis, P. pinaster plants were inoculated with the PWN, treated with copper oxide (CO) or potassium phosphonate (PP), and evaluated post-inoculation for: i) the extent of foliar symptoms; ii) nematode density inside stem tissues; iii) proxies for oxidative damage and antioxidant activity, iv) mineral concentration; and v) bacterial diversity. The mortality of infected plants reached 12.5% regardless of the treatment, but plants treated with fungicides, particularly with PP, had significantly lower PWN density (up to 0.61-fold). Plants treated with PP had substantially higher concentrations of anthocyanins at 14 dai than those treated with CO and non-Treated plants (by 1.47-fold), possibly contributing to the lower PWN colonization and degree of foliar symptoms observed. CO and PP led to increased lipid peroxidation at 28 dai (by 1.84- and 1.77-fold), and PP showed higher flavonoids concentration than CO (by 1.37- and 0.49-fold), corroborating its higher potential in increasing plant antioxidative response during infection. Fungicides also induced significant changes in micronutrient accumulation in plant tissues, resulting in a decrease in Zn and P concentrations in plants treated with either fungicide as compared to infected non-treated plants. Finally, CO treatment increased the diversity of the bacterial communities, while PP decreased microbial biodiversity. Altogether, results suggest that treatment with CO and PP increases tolerance against B. xylophilus by promoting the plant antioxidant system, changing the accumulation of essential minerals, and modulating plant-associated bacterial diversity.
An eConsultant versus a hospital-based outpatient consultation for general (internal) medicine: a costing analysis
Authors: Job Jennifer , Nicholson Caroline , Donald Maria , Jackson Claire , Byrnes Joshua 
Published: March 2023

Abstract
Background
The eConsultant model of care is an outpatient substitution approach which has been evaluated and implemented extensively internationally. It provides an asynchronous, digital, clinician-to-clinician advice service, giving primary care physicians remote access to specialist support for patient care within 3 business days. Results from initial trials of the eConsultant model in Australia support international evidence of reduced wait times and improved access to specialist input, avoidance of face-to-face hospital outpatient visits, and better integrated care. This study compared the cost of delivery of an eConsultant episode of care with that of a hospital-based outpatient appointment.
Methods
A cost analysis, using a decision analytic model, was used to compare the two approaches. eConsultant costs were calculated from specialist reported data (minutes spent preparing the response; the number of patients referred subsequently for a hospital-based outpatient appointment) and administration staff data (time spent recording the occasion-of-service). Outpatient costs were calculated using finance data and information from outpatient clinic managers at the hospital-based outpatient clinic. The primary outcome was incremental cost saving per patient from a health system perspective. Uncertainty was explored using one-way sensitivity analyses and characterised with probabilistic sensitivity analysis using 10,000 Monte Carlo simulations.
Results
The traditional referral pathway cost estimate was $587.20/consult compared to $226.13/consult for an eConsultant episode: an efficiency saving of $361.07 per patient. The incremental difference between eConsultant and traditional care was most sensitive to the cost estimate of an outpatient attendance, the time for a specialist to complete an eConsult, and the probability of a patient requiring a face-to-face hospital-based attendance following an eConsult. However, at the upper bounds of each of these estimates, an eConsult remained the most cost-efficient model. In 96.5% of the Monte Carlo simulations eConsult was found to be more cost efficient than the traditional approach.
Conclusions
The eConsultant model of care was associated with a 61.5% efficiency gain, allowing diversion of support to hospital-based outpatient appointments.
Bifunctional protein ArsRM contributes to arsenite methylation and resistance in Brevundimonas sp. M20
Authors: Li Congcong , Zong Gongli , Gao Wenhui , Chen Xi , Tan Meixia , Fu Jiafang , Zhang Peipei , Wang Bing , Cao Guangxiang 
Published: March 2023

Abstract
Background
Arsenic (As) with various chemical forms is the most prevalent water and environmental toxin. This metalloid occurs worldwide and causes various diseases including cancer, especially arsenite [As(III)]. Organification of arsenite is an effective way for organisms to cope with arsenic toxicity. Microbial communities have been shown to be a vital contributor in the global arsenic biocycle and to be a promising way to reduce arsenite toxicity.
Methods
Brevundimonas sp M20 with arsenite resistance was isolated from aquaculture sewage. arsHRNBC cluster and the metRFHH operon of M20 was identified by sequencing. Gene of ArsR/methyltransferase fusion protein, arsRM, was amplified and expressed in Escherichia coli BL21 (DE3) and resistance to arsenic was determined in present of 0.25–6 mM As(III), As(V), or Rox(V). Methylation and regulatory details of ArsRM were analyzed by Discovery Studio 2.0. Then methyltransferase activity analysis and EMSAs confirmed the functions.
Results
MIC of Brevundimonas sp. M20 to arsenite is 4.5 mM. A 3,011-bp arsenite resistant ars cluster arsHRNBC and a 5649-bp methionine biosynthesis met operon were found on the 3.315-Mb chromosome. Transcriptional regulator/methyltransferase fusion protein ArsRM is a difunctional protein. Expression of ArsRM in E. coli increased arsenite resistant to 1.5 mM. Arsenite methylation and binding to its own promoter region was confirmed by ArsRM. The As(III)-binding site (ABS) and SAM-binding motif provide the difunctional characteristic to ArsRM.
Conclusions
We conclude that ArsRM promotes the arsenite methylation and binding of to its own promoter region. This difunctional characteristic directly connects methionine and arsenic metabolism. Our findings add important knowledge about microbial arsenic resistance and detoxification. Future work should further explore the regulatory mechanism of ArsRM on met operon and ars cluster.
Effect of potting media on plant growth and flower yield in Dendrobium orchid cv. Sonia Red under High-Tech polyhouse
Authors: Kaur Rajveer , Singh Parminder 
Published: March 2023

Abstract
The study was conducted to determine the “Effect of potting media on plant growth and flower yield in Dendrobium orchid cv. Sonia Red” under hi-tech polyhouse at Punjab Agricultural University, Ludhiana, Punjab during 2021-22. Tissue cultured plants of Dendrobium orchid were grown in fifteen potting media treatment combinations comprising of Cocopeat, Coco hull, Plywood industry waste, Brick pieces, Maize rind, Vermicompost and Charcoal. Data was recorded at quarterly intervals. Among all the potting media treatments, T10 (Maize rind + Vermicompost + Charcoal - 2:1:1) number of florets per flower stem, spike length, rachis length, longevity of flower stems and vase life of flowers. However,treatment T6 exhibited maximum number of flower stems per plant. Teatments T5 (Plywood industry waste + Vermicompost + Charcoal - 3:1:1) and T4 (Plywood industry waste + Vermicompost + Charcoal - 2:1:1) exhibited minimum number of days for spike initiation, flower bud initiation, bud opening to harvest stage. To conclude potting media T10 was found to be better in terms of floral parameters.
AE analysis of mode II interlaminarfracture toughness of 3D reinforced CFRP
Authors: Oliveira Thiago Luiz Lara , Bortoluzzi Daniel Brighenti , Barbosa Lorena Cristina Miranda , Ancelotti Antônio Carlos 
Published: March 2023

Abstract
The industrial application of composite materials is increasing, showing its viability to replace other materials have been gradually growing. Particularly, composite materials reinforced with carbon fibres have a good ratio of resistance/weight, being an advantage for many applications since Carbon Fibre Reinforced Polymers (CFRP) allows reducing weight and keeping structural resistance. Resistance to delamination damage is a key factor for composite materials, since it deals with material safety during service life. Fibre orientation in composite materials is designed to reinforce resistance in specific directions of severe solicitation. Nonetheless, out-of-plane loadings are inherent solicitations that occurs in service life and fibre orientation are not intended to reinforce thought thickness direction, making out-of-plane loadings a critical factor. Reinforcing composite materials through thickness, or 3D, has the potential to increase the material out-of-plane resistance and thus permitting a higher accuracy levels in terms of structural design. There are different ways to apply 3D reinforcement on composite materials, like Z-pinning, Stitching and Tufting. Non-destructive testing (NDT) are efficient methods of reassuring safety of components, NDT allows detection of damage and flaws in early stages and monitoring of a component. NDT methods are particular useful for composite materials for avoiding parts containing flaws, indicating repairs to increase lifespan and for lifespan estimation. Acoustic emission is one technique of NDT (non-destructive testing) that allows monitoring of damage evolution in real time, since it detects elastic waves emitted by energy release caused by changes in internal structure, for NDT purposes it means damage. This paper monitors and evaluates the signs generated during the mode II interlaminar fracture toughness test, end-notched flexure (ENF), monitored by acoustic emission comparing the effect of different though thickness reinforcements and a non-reinforced sample. The testing procedure and damage development are analysed regarding acoustic emission features and its behaviour. The results show that acoustic emission has a strong relationship with stages of testing, and samples resistance induced by the reinforcement. The data acquired from the ENF tests in synergy with acoustic emission provided damage characterization and differentiation, reaffirmed by fractography analysis, especially in terms of final stage of damage and 3D reinforcement effects on it.
Single Relaxation Time and Multiple Revised Matrix Lattice Boltzmann Simulations of Forced Isotropic Turbulence
Authors: Kareem Waleed Abdel , Izawa Seiichiro , Fukunishi Yu 
Published: March 2023

Abstract
The single relaxation time (SRT) and the revised matrix (RM) lattice Boltzmann models are compared for simulations of three dimensional forced isotropic turbulence with resolutions of 1283 and 2563, respectively. Guo et al.[1] forcing technique with the same forcing parameters and conditions is used to investigate the ability and stability of the two models for simulations of forced turbulence. Some new aspects and results have been confirmed such as the superiority of the MRT model to simulate forced turbulence in comparison with the SRT model. To overcome the stability shortage problem of the lattice Boltzmann models, it is proposed to apply the Courant-Friedichs-Lewy condition (CFL) [2] by multiplying the velocity input with the CFL coefficient which is less than unity and by dividing the output velocity data by the same CFL coeffi-cent. The initial velocity field is chosen as u(x, 0) = 0 and the force is injected at low wave-numbers with a fixed forcing amplitude of 10−4 for all cases. The single relaxation time is set to τ = 0.503 in all SRT simulations. Results show that the obtained turbulent velocity fields yield universal characteristics as proven in previous theoretical, experimental and numerical studies. The Taylor Reynolds number for the simulations are found as : Rλ(SRT) = 62 and Rλ(M RT) = 65 for the resolution of 1283 and Rλ(SRT) = 107 and Rλ(M RT) = 82 for the case of 2563, respectively. To test the weak incompressibility for the SRT model in comparison to the MRT model, the probability distribution function (PDF) of the density ρ is depicted and it is found that ρ is almost unity at all timesteps for the MRT case, while a clear disturbance about unity is observed for the SRT case. Time variation’s statistics such as the Taylor Reynolds number Rλ, the Taylor microscale λ, the Kolmogrov microscale η and energy spectra are calculated and depicted for all cases. One of the disadvantages of the MRT case is the simulations time which is found about twice the SRT simulations time, where about 80 hours are needed for simulating 12000 timesteps in the 2563 case in comparison to 42 hours for the SRT model using the same hardware resources.
SIGAA – SIGnaling Automated Analysis: a new tool for Ca2+ signaling quantification using ratiometric Ca2+ dyes
Authors: Lopes Rafael Faria , Gonçalves-Ribeiro Joana , Sebastião Ana M. , Meneses Carlos , Vaz Sandra H. 
Published: March 2023

Abstract
Astrocytes are non-neural cells, restricted to the brain and spinal cord, whose functions and morphology depend on their location. Astrocyte-astrocyte and astrocyte-neuron interactions occur through cytoplasmic Ca2+ levels changes, that are assessed to determine cell function and response (i.e. drug testing). Evaluation of changes in intracellular Ca2+ levels is mostly centered on fluorescence imaging approaches, performed through video recording of cells incubated with Ca2+-sensitive dyes. By observing ion concentration shifts over time in a delimited region of interest (ROI) comprising a single cell, it is possible to attain conclusions on cell responses to specific stimuli. Our work describes a tool named SIGAA – SIGnaling Automated Analysis, for astrocyte ROI-based fluorescent imaging, which is tailored for two wavelengths dyes by using two inputs of Ca2+ signaling recorded frames/videos and outputs a set of features relevant to the experiment’s conclusions and cell characterization. SIGAA performs automatic drift correction for the two recorded videos with a template matching algorithm, followed by astrocyte identification (ROI) using morphological reconstruction techniques. SIGAA then extracts intracellular Ca2+ evolution functions for all identified ROIs, detects function transients, and estimates a set of features for each signal, which are very similar to the ones obtained by the traditional methods and software used so far. SIGAA is a new fully automated tool, which can speed up hour-long studies and analysis to a few minutes, showing reliable results as the validity tests indicate.
Anemia and Iron Dosing Supplementation Using Reticulocyte-Hemoglobin (Ret-He) in the NICU
Authors: Ali Sara , Fuenmayor Maria Franco , Huff Monica , Burdine Jordan , Meaux Katelyn Harris 
Published: March 2023

Abstract

Preterm infants are at high risk for iron-deficiency anemia necessitating treatment with a blood transfusion. Research regarding iron utilization and blood transfusions in the NICU has centered on the incidence, physiology, effects, and prevention of anemia in high-risks neonates and the potential to improve neurodevelopmental outcomes. Predictors of blood transfusion in the premature and very or extremely low birth weight infant include gestational age, initial hemoglobin level, phlebotomy losses and low endogenous erythropoietin. Enteral supplementation with iron is the standard of care. Several studies have addressed the ideal iron supplementation dosing for preterm infants comparing 2 to 4mg/kg/day of enteral iron until 8 weeks postnatal age. Higher iron dosing has been utilized in trials using erythropoietin supplementation. Ret-Hemoglobin (Ret-He) has emerged as a marker for iron sufficiency in preterm infants. With supplementation, there appears to be improved iron storage, less anemia, and a potential positive effect on neurodevelopment.
Advanced hepatocellular carcinoma with MET-amplified contained excellent response to Crizotinib: a case report.
Authors: Gu Yangjun , Xiao Min , Chen Zhitao , Li Qiyong 
Published: March 2023

Abstract
Background:Hepatocellular carcinoma (HCC) is one of the most lethal tumors all over the world. It’s considered with extensive heterogeneity and lacks therapeutic decision-making biomarkers. There are many novel therapeutic strategies tried to prolong the survival of advanced HCC. Next-generation sequencing (NGS) is generally used for helping treatment decision which help patient benefit from genome-directed targeted.
Case presentation:A 56 years-old male with more than 20 years of type-B hepatitis was admitted to our department who underwent laparoscopic hepatic left lateral lobectomy because hepatocellular carcinoma. Unfortunately, tumor recurrence 1 year later. Though multiple treatments were taken, including interventional therapy, tyrosine kinase inhibitor, radiotherapy and immunotherapy, the tumor invaded his 5th thoracic vertebras and leaded to hypoesthesia and hypokinesia below the nipple line plane 2 years later. NGS was used to find potential therapeutic biomarkers. As the met amplification was identified, Crizotinib, the inhibitor of the C-Met was recommended. Tumor reduction and decreased tumor marker were observed immediately in 1 month later. And the patient has remained on remission since then.
Conclusions: We reported a patient with high MET amplification benefited from MET inhibitors which recommended by NGS. It indicated the potential clinical decision support value of NGS and the satisfactory treatment effect of C-met inhibitor.
Effects of pyrolyzing and phosphiding on dibenzothiophene hydrodesulfurization of MOF-derived Ni2P
Authors: Liu Yanyan , Wang Zhiheng , Li Xin , Gao Ruiyao , Sun Jiong , Yao Songdong 
Published: March 2023

Abstract

MOF template, MOF-derived Ni, and MOF-derived Ni2P were characterized by XRD, TG, SEM, TEM, XPS and Raman. Hydrodesulfurization (HDS) of dibenzothiophene of MOF-derived Ni2P suggests that its catalytic performance depends on the active center number of Ni2P (111), the size of Ni2P clusters, the phosphiding degree of Ni and the intervals between Ni2P clusters. Reducing the pyrolyzing temperature of MOF template can slow down particle aggregation and change the morphology of Ni and Ni2P from closely packed polyhedron to loosely packed petal-like. At the same time, the cluster size of Ni2P was significantly reduced, resulting in a higher HDS activity. Prolonging pyrolyzing time at low pyrolyzing temperature leads to the gradual removal of MOF framework and makes nickel aggregates more susceptible to phosphiding. This increases the ratio of Ni2P to Ni of the phosphidized material, resulting in higher HDS activity of MOF-derived Ni2P. In situ phosphiding of crystalline nickel with PH3 did not change the shape of aggregates, but change the surface morphology of MOF derivied Ni. Low-temperature phosphiding can generally form pure Ni2P species, while high-temperature phosphiding will form a variety of impurities including Ni5P4. This will lead to a decrease in HDS reaction performance of MOF-derived Ni2P catalyst.
False Position Method for Solving Fuzzy Non-Linear Equation Using Triangular and Trapezoidal Fuzzy Number
Authors: Akter Mst. Monjuara , Hossain Md. Sahadat 
Published: March 2023

Abstract
In this paper, we derive the false position method for solving fuzzy non-linear equations using triangular and trapezoidal fuzzy numbers. In particular, two techniques for obtaining the roots of a fuzzy nonlinear equation using a triangular fuzzy number are shown, and the optimal solution is determined by comparing the two methods which are best between them. Included are numerical examples with graphical representations to show the efficacy of the proposed method. Finally, we used this best method to solve fuzzy non-linear equations using trapezoidal fuzzy numbers.
Learning and adaptation：A comparison of traditional plant knowledge between Daman people and Tibetans in Gyirong River Valley, Tibet, China
Authors: Guo Chang-An , Ding Xiaoyong , Hu Huabin , Zhang Yu , Wang Yuhua 
Published: March 2023

Abstract
Background
"Diaspora" refers to a specific group of immigrants that have settled in different regions throughout history. In Gyirong town, Tibet, China, there exists such an ethnic group known as the "Daman people". In 2003, they were granted Chinese citizenship. The objective of this study is to document the traditional knowledge of plant use by the Daman people in Gyirong town. It aims to explore the extent to which the Daman people have incorporated local Tibetan plant use practices and whether they maintain their own distinct knowledge of plant use.
Methods
During fieldwork, ethnobotanical data were collected using various methods including free listings, key informant interviews, and semi-structured interviews. To quantify the importance of plant species in the Daman people's culture, the culture importance index (CII), informant consensus factor index (FIC), and The Index of Agreement on Species consensus (IASc) were used. To more comprehensively compare the differences in plant use between the Daman and Tibetan, this study constructed a knowledge network to compare the knowledge differences between the two groups.
Results
In this study, traditional knowledge was collected from 32 Daman informants and 126 Tibetan informants, resulting in a total of 68 species belonging to 39 families mentioned by Daman people and 111 species mentioned by Tibetans. Of these, 58 plants were used by both populations. The plants were classified into 3 first categories and 28 second categories, with 22 identical classes in both groups. The majority of use categories showed a high degree of sharing in both groups, and the Tibetan people had more plant use categories than the Daman people. Five plants with IASc value > 0.5 were identified in both groups: Rhododendron anthopogon D. Don, Artemisia japonica Thunb., Juniperus indica Bertol., Gastrodia elata Blume, and Rheum australe D. Don. The analysis of the knowledge network revealed a 66% overlap between the knowledge of the Daman and the knowledge of the Tibetans. Additionally, the plant knowledge of Tibetan people was found to be richer and more complex than that of the Daman people. However, the Daman people possess 30 unique knowledge items.
Conclusions
From the perspective of plant use, the history of the Daman people's discrete migration on the border between China's Tibet and Nepal allows them to retain their own knowledge of plant use. The status quo of joining Chinese nationality and settling in Gyirong town allows them to gradually integrate into the local Tibetan society. In constant communication with the Tibetan culture, the Daman acquired a great deal of traditional plant knowledge, so that they could better adapt to the natural environment of Gyirong.