bluuebunny/crossref_metadata_2025_split huggingface dataset:
Evaluation of Proximate, Phytochemical, and Heavy Metal Content in Black Cumin and Fenugreek Cultivated in Gamo Zone, Ethiopia
Authors: Tura Alemu Mekonnen , Debisa Markos Dele , Tulu Etalema Desta , Tilinti Birhanu Zeleke 
Published: January 2023

Spices have been recognized to have medicinal properties. Among several spices and medicinal plants, black cumin and fenugreek are very common. Geographical, climatic, and cultivating soil type can change the chemical composition of these spices. The objective of this work is to determine proximate, phytochemical, and heavy metal content in black cumin and fenugreek cultivated in the Gamo zone, Ethiopia. The proximate composition (moisture, ash, fiber, fat, protein carbohydrate, and gross energy) was determined; their content (%) in black cumin is

6.98
±
0.12

,

5.02
±
2.9

,

6.90
±
0.21

,

32.1
±
0.68

,

20.9
±
0.09

,

34.1
±
0.73

, and

498
±
4.4

, respectively, and

6.30
±
0.35

,

4.27
±
0.17

,

9.36
±
0.25

,

12.8
±
0.41

,

30.8
±
0.09

,

46.1
±
0.52

, and

422
±
1.6

, respectively, in fenugreek. The total means of phenolic flavonoids and alkaloids in black cumin are

193
±
5.3

mg

GAE
/
100

g

,

87.6
±
4.3

mg

QE
/
100

g

, and

69.4
±
4.7

mg

AE
/
100

g

, respectively, and

382
±
11

mg

GAE
/
100

g

,

123
±
3.6

mg

QE
/
100

g

, and

37.6
±
2.2

mg

AE
/
100

g

, respectively, in fenugreek. In black cumin and fenugreek collected from Kamba, Daramalo, and Dita woredas, nine heavy metals were determined by using FAAS. The total mean concentrations in mg/kg for detected heavy metals (Fe, Zn, Cu, Mn, and Co) in black cumin are

78.5
±
5.9

,

27.3
±
1.5

,

3.06
±
0.26

,

8.47
±
0.22

, and

10.1
±
0.37

, respectively, and Cr, Ni, Pb, and Cd were not detected in black cumin. Similarly, the concentrations of Fe, Zn, Cu, Mn, Co, and Ni in fenugreek are

168
±
5.2

,

14.8
±
0.20

,

4.76
±
0.1

,

13.7
±
0.40

,

3.66
±
0.21

, and

2.12
±
0.03

, respectively, and Cr, Pb, and Cd were not detected in fenugreek. All the results of determined parameters were compared to previous studies, and the values were in agreement with slight variations. Therefore, black cumin and fenugreek cultivated in the Gamo zone were free from heavy metal toxicity.
Data-driven Cross-lingual Syntax: An Agreement Study with Massively Multilingual Models
Authors: Varda Andrea Gregor de , Marelli Marco 
Published: January 2023

Abstract
Massively multilingual models such as mBERT and XLM-R are increasingly valued in Natural Language Processing research and applications, due to their ability to tackle the uneven distribution of resources available for different languages. The models’ ability to process multiple languages relying on a shared set of parameters raises the question of whether the grammatical knowledge they extracted during pre-training can be considered as a data-driven cross-lingual grammar. The present work studies the inner workings of mBERT and XLM-R in order to test the cross-lingual consistency of the individual neural units that respond to a precise syntactic phenomenon, that is, number agreement, in five languages (English, German, French, Hebrew, Russian). We found that there is a significant overlap in the latent dimensions that encode agreement across the languages we considered. This overlap is larger (a) for long- vis-à-vis short-distance agreement and (b) when considering XLM-R as compared to mBERT, and peaks in the intermediate layers of the network. We further show that a small set of syntax-sensitive neurons can capture agreement violations across languages; however, their contribution is not decisive in agreement processing.
An information-theoretic analysis of resting-state versus task fMRI
Authors: Tuominen Julia , Specht Karsten , Vaisvilaite Liucija , Zeidman Peter 
Published: January 2023

Abstract
Resting-state fMRI is an increasingly popular alternative to task-based fMRI. However, a formal quantification of the amount of information provided by resting-state fMRI as opposed to active task conditions about neural responses is lacking. We conducted a systematic comparison of the quality of inferences derived from a resting-state and a task fMRI paradigm by means of Bayesian Data Comparison. In this framework, data quality is formally quantified in information-theoretic terms as the precision and amount of information provided by the data on the parameters of interest. Parameters of effective connectivity, estimated from the cross-spectral densities of resting-state- and task time series by means of dynamic causal modelling (DCM), were subjected to the analysis. Data from 50 individuals undergoing resting-state and a Theory-of-Mind task were compared, both datasets provided by the Human Connectome Project. A threshold of very strong evidence was reached in favour of the Theory-of-Mind task (&gt;10 bits or natural units) regarding information gain, which could be attributed to the active task condition eliciting stronger effective connectivity. Extending these analyses to other tasks and cognitive systems will reveal whether the superior informative value of task-based fMRI observed here is case specific or a more general trend.
The transformation of medical research in Mexico: A structural analysis of thematic domains, institutional affiliations, authors’ cohorts, and possible correlations
Authors: Milia Matías Federico , Brambila Claudia Gonzalez , Lee Ángel , Ponce José Ignacio 
Published: January 2023

Abstract
Global research on medical and health-related issues has experienced a profound reconfiguration over the last 30 years. The rise of new areas of inquiry has transformed the medical research landscape as staff with medical training gradually relinquished their prominence and specialists from other disciplines raised their profile within research teams. Given this, research priorities seem to be shifting increasingly towards laboratory-based and innovation-oriented research lines. The unfolding of these shifts in nonhegemonic countries such as Mexico is still to be understood. This paper surveys structural changes in Mexican medical research from 1993 to 2021 by observing temporal aggregation of authorships, emerging thematic features, and institutional affiliation patterns. It also explores correlations between these findings and their possible explanations. The results allow us to empirically describe significant changes in medical research done in Mexico. We detected periods of stability in authorship allowing us to describe stages in the accumulation of research and development (R&D) capabilities. The identified semantic patterns allowed us to characterize this transformation, observing subsequent stages of an accumulation and specialization process that began in the mid-1990s. Moreover, we found divergent thematic and institutional patterns that point towards a growing gap between research conducted in health institutions and scientific ones.
Empirical Theology as Theological Netnography: Methodological Considerations
Authors: Cartledge Mark J. 
Published: January 2023

Abstract
It is clear that the role of the internet is ubiquitous in everyday life. With smart phones almost everyone can access the internet 24/7 and use it for emails, social network activity, searching for information and navigation when driving a car to a new destination. It is also the case that the online world intersects directly with and is embedded in the off-line world of our everyday lives. Almost all forms of religion have this intersection and function with it as a matter of course. For Christians this has meant that ecclesial dimensions of spiritual life have also been taken online, or that the online dimensions have intersected with the everyday ecclesial life of most if not all Christians. The Covid-19 pandemic demonstrated how easily it was for most church congregations to arrange for services to be live-streamed online, supported by social media. Indeed, these new types of services attracted many visitors during the various lockdowns and a number of regular church-goers decided that they preferred the online world to the concrete one they were previously familiar. These recent changes in religious life invite reflection from the perspective of empirical theology. In particular, it may be asked how might an empirical approach to theology develop in relation to virtual ethnography or netnography? There is an emerging body of methodological literature that embraces the study of online or digital communities, otherwise known as netnography (Boellstorff et al, 2012; Hine, 2000, 2015; Kozinets, 2013, 2020; Pink et al, 2016), but, so far, there is a very limited application of empirical studies from a theological perspective (although see Hutchings, 2017; Sutinen and Cooper, 2021). Therefore, this paper explores the question: is there a distinctly empirical-theological approach to netnography? This question will be addressed in this exploratory paper and a methodological proposal suggested as part of the discussion.
Modulation Effect of the Annual Cycle on Interdecadal Warming Trends over the Tibetan Plateau during 1998–2020
Authors: Qin Zhengkun , Zou Xiaolei 
Published: January 2023

Abstract
The Tibetan Plateau is a sensitive area of global climate change, where few conventional observations exist. Satellite AMSU-A microwave temperature sounding observations of brightness temperature (TB) are located in the absorption band of oxygen, which is well mixed in the atmosphere, and have microwave frequencies varying from 50.3 to 57.6 GHz. Therefore, AMSU-A TB observations at different sounding channels reflect atmospheric temperatures at different altitudes. In this study, AMSU-A TB observations during 1998–2020 from five polar-orbiting environmental meteorological satellites (POESs) are employed to investigate the interdecadal warming/cooling trends over the Tibetan Plateau. A limb correction is first applied to all AMSU-A channels before using TB observations at all fields of view for examining geographic distributions and differences of global warming/cooling trends. It is found that interdecadal trends of upper-tropospheric warming and stratospheric cooling are stronger over the Qinghai Tibetan Plateau than its eastern plain areas. An interdecadal variation of the annual cycle over the Tibetan Plateau is an important factor for the enhanced tropospheric warming trend. We also applied a different approach of significance testing that is based on counting signs of local trends (sign test) and confirmed that the detected significant local trends were not a result of chance. In addition, high-frequency noise in TB observations with periods smaller than annual and semiannual oscillations do not affect the climate trends of TB very much, but significantly reduced the uncertainty of the TB trends over the Tibetan Plateau.
Methods for Estimating Surface Water Storage Changes and Their Evaluations
Authors: Wang Shusen , Li Junhua , Russell Hazen A. J. 
Published: January 2023

Abstract
Developing effective methods for estimating regional-scale surface water storage change (ΔSW) has become increasingly important for water resources studies and environmental impact assessment. Three methods for estimating monthly ΔSW are proposed in this study, of which one is based on land surface runoff and two that use water body water budgets. Water areas observed by Landsat satellites for Canada’s entire landmass are used for evaluation of the results. The surface runoff method achieved the least satisfactory results, with large errors in the cold season or dry regions. The two water-budget methods demonstrated significant improvements, particularly when water area dynamics is considered in the estimation of the water body water budget. The three methods performed consistently across different climate regions in the country and showed better correlations with observations over wet climate regions than over dry regions with poorly connected hydrological system. The results also showed the impact of glacier and permanent snow melts over the Rocky Mountains on basin-scale surface water dynamics. The methods and outputs from this study can be used for calibrating and validating hydrological and climate models, assessing climate change and human disturbance impacts on regional water resources, and filling the ΔSW data gaps in GRACE-based total water storage decompositions studies.

Significance Statement
The purpose of this study is to develop and evaluate methods for estimating regional-scale surface water storage change. This is important because information on surface water dynamics is limited for water resources studies and environmental impact assessment. Our study makes available two new methods which significantly improve on surface water storage estimation from the traditional runoff model. A guide on controls of surface water dynamics is provided for regions under various hydroclimate and physiographic–hydraulic conditions and reveals the influence of glacier melt on surface water variations.
Sports-Related Concussions and Adverse Health Behaviors Among Middle and High School Students
Authors: Sarmiento Kelly , Miller Gabrielle F. , Jones Sherry Everett 
Published: January 2023

Background: Concussions affect millions of youths in the United States each year, and there is concern about long-term health effects from this injury. Purpose: To examine the association between sports- or physical activity–related concussion and health risk behaviors among middle and high school students in 9 states. Study Design: Cross-sectional study; Level of evidence, 3. Methods: Data from the 2019 middle school and high school Youth Risk Behavior Survey were used for this analysis. Nine states were identified that included the same question on concussion and similar questions on health risk behaviors in their 2019 Youth Risk Behavior Survey. Students were asked to self-report whether they had ≥1 sports- or physical activity–related concussions during the 12 months preceding the survey. Self-reported concussion was the primary outcome of interest. Other variables included sex, race/ethnicity, played on a sports team, were physically active 5 or more days/week, ever tried cigarette smoking, ever used an electronic vapor product, academic grades, drank alcohol, were in a physical fight, seriously considered attempting suicide, made a suicide plan, and attempted suicide. Results: Among the 9 states, 18.2% of middle school students and 14.3% of high school students self-reported ≥1 sports- or physical activity–related concussions. Among both middle school and high school students, the prevalence of ≥1 sports- or physical activity–related concussions was higher among students who played on a sports team, were physically active 5 or more days per week, had ever tried cigarette smoking, had ever used an electronic vapor product, had seriously considered attempting suicide, had made a suicide plan, and had attempted suicide compared with those who had not engaged in those behaviors. The prevalence of sports- or physical activity–related concussion was consistently higher among middle school students than high school students across sex, race/ethnicity, and adverse health behaviors. Conclusion: Middle school students with a history of concussion warrant attention as an at-risk population for concussions and adverse health behaviors. Health care providers may consider screening students for adverse health behaviors during preparticipation examinations and concussion evaluations.
Betalain biosynthesis in red pulp pitaya is regulated via HuMYB132: a R-R type MYB transcription factor
Authors: Xie Fangfang , Chen Canbin , Chen Jiayi , Chen Jiaxuan , Hua Qingzhu , Shah Kamran , Zhang Zhike , Zhao Jietang , Hu Guibing , Chen Jianye , Qin Yonghua 
Published: January 2023

Abstract
Background
Multiple MYB transcription factors (TFs) are involved in the regulation of plant coloring. Betalain is a kind of natural plant pigment and its biosynthesis is regulated by a number of enzymes. Despite this, little is known about the molecular properties and roles of MYB TFs in pitaya betalain biosynthesis.

Results
In the present study, we identified a 1R-MYB gene, HuMYB132, which is preferentially expressed in red-pulp pitaya at the mature stage. It was clustered with Arabidopsis R-R-type genes and had two DNA-binding domains and a histidine-rich region. The expression assays in N. benthamiana and yeast indicated that HuMYB132 is a nucleus-localized protein with transcriptional activation activity. Dual luciferase reporter assay and electrophoretic mobility shift assays (EMSA) demonstrated that HuMYB132 could promote the transcriptional activities of HuADH1, HuCYP76AD1–1, and HuDODA1 by binding to their promoters. Silencing HuMYB132 reduced betalain accumulation and the expression levels of betalain biosynthetic genes in pitaya pulps.

Conclusions
According to our findings, HuMYB132, a R-R type member of 1R-MYB TF subfamily, positively regulates pitaya betalain biosynthesis by regulating the expression of HuADH1, HuCYP76AD1–1, and HuDODA1. The present study provides a new theoretical reference for the management of pitaya betalain biosynthesis and also provides an essential basis for future regulation of betalain biosynthesis in Hylocereus.
Primary head and neck cancer cell cultures are susceptible to proliferation of Epstein-Barr virus infected lymphocytes
Authors: Shao Senyao , Scholtz Lars Uwe , Gendreizig Sarah , Martínez-Ruiz Laura , Florido Javier , Escames Germaine , Schürmann Matthias , Hain Carsten , Hose Leonie , Mentz Almut , Schmidt Pascal , Wang Menghang , Goon Peter , Wehmeier Michael , Brasch Frank , Kalinowski Jörn , Oppel Felix , Sudhoff Holger 
Published: January 2023

Abstract
Background
New concepts for a more effective anti-cancer therapy are urgently needed. Experimental flaws represent a major counter player of this development and lead to inaccurate and unreproducible data as well as unsuccessful translation of research approaches into clinics. In a previous study we have created epithelial cell cultures from head and neck squamous cell carcinoma (HNSCC) tissue.

Methods
We characterize primary cell populations isolated from human papillomavirus positive HNSCC tissue for their marker expression by RT-qPCR, flow cytometry, and immunofluorescence staining. Their sensitivity to MDM2-inhibition was measured using cell viability assays.

Results
Primary HNSCC cell cultures showed the delayed formation of spheroids at higher passages. These spheroids mimicked the morphology and growth characteristics of other established HNSCC spheroid models. However, expression of epithelial and mesenchymal markers could not be detected in these cells despite the presence of the HNSCC stem cell marker aldehyde dehydrogenase 1 family member A1. Instead, strong expression of B- and T-lymphocytes markers was observed. Flow cytometry analysis revealed a heterogeneous mixture of CD3 + /CD25 + T-lymphocytes and CD19 + B-lymphocytes at a ratio of 4:1 at passage 5 and transformed lymphocytes at late passages (≥ passage 12) with CD45 + CD19 + CD20 + , of which around 10 to 20% were CD3 + CD25 + CD56 + . Interestingly, the whole population was FOXP3-positive indicative of regulatory B-cells (Bregs). Expression of transcripts specific for the Epstein-Barr-virus (EBV) was detected to increase in these spheroid cells along late passages, and this population was vulnerable to MDM2 inhibition. HPV + HNSCC cells but not EBV + lymphocytes were detected to engraft into immunodeficient mice.

Conclusions
In this study we present a primary cell culture of EBV-infected tumor-infiltrating B-lymphocytes, which could be used to study the role of these cells in tumor biology in future research projects. Moreover, by describing the detailed characteristics of these cells, we aim to caution other researchers in the HNSCC field to test for EBV-infected lymphocyte contaminations in primary cell cultures ahead of further experiments. Especially researchers who are interested in TIL-based adopted immunotherapy should exclude these cells in their primary tumor models, e.g. by MDM2-inhibitor treatment. BI-12-derived xenograft tumors represent a suitable model for in vivo targeting studies.
25-hydroxyvitamin D3 inhibits oxidative stress and ferroptosis in retinal microvascular endothelial cells induced by high glucose through down-regulation of miR-93
Authors: Zhan Dongmei , Zhao Juan , Shi Qin , Lou Juan , Wang Weiling 
Published: January 2023

Abstract
Background
The decrease of vitamin D plays a critical role in diabetes mellitus (DM)-induced oxidative stress and vascular endothelial injury. Therefore, we investigated the effect and mechanism of 25-hydroxyvitamin D3 (25 (OH) D3) on oxidative stress and ferroptosis induced by high glucose in human retinal microvascular endothelial cells (hRMVECs). And the objective of this paper was to propose a new strategy for the prevention and treatment of diabetic retinopathy (DR).

Methods
First, hRMVECs were transfected with mimics NC or miR-93. After that, cells were treated with 100 nM / 500 nM 25 (OH) D3 and then cultured in a high glucose (30 mM) environment. Subsequently, qRT-PCR was employed to detect the expression level of miR-93; CCK-8 for the proliferation of cells in each group; biochemical tests for the level of intracellular reactive oxygen species (ROS), malondialdehyde (MDA), reduced glutathione (GSH) and ferrous ion (Fe2+); and Western blot for the expression of ferroptosis-related proteins glutathione peroxidase 4 (GPX4) and SLC7A11).

Results
Under a high glucose environment, 25 (OH) D3 at 100 nM/500 nM could significantly promote the proliferation of hRMVECs, remarkably decrease the level of intracellular ROS/MDA, and up-regulate the level of GSH. Besides, 25 (OH) D3 greatly reduced Fe2+ level in the cells while increased protein level of GPX4 and SLC7A11. Subsequently, we found that high glucose induced miR-93 expression, while 25 (OH) D3 markedly decreased high glucose-induced miR-93 overexpression. Furthermore, overexpression of miR-93 inhibited the functions of 25 (OH) D3 by activating ROS (ROS and MDA were up-regulated while GSH was down-regulated) and inducing Fe2+ (Fe2+ level was up-regulated while GPX4 and SLC7A11 level was down-regulated) in cells.

Conclusion
25 (OH) D3 may inhibit oxidative stress and ferroptosis in hRMVECs induced by high glucose via down-regulation of miR-93.