bluuebunny/crossref_metadata_2025_split huggingface dataset:
Fiscal policy and the twin deficits: structural changes matter
Authors: Kim Wongi 
Published: March 2023

AbstractThis paper empirically and theoretically investigates the relationship between budget balances and external balances, the so-called twin deficit hypothesis. Using the US post-World War II data, I estimate a time-varying structural vector autoregressive model to evaluate the effects of structural breaks on this relationship. The empirical results reveal that the relationship is significantly time varying: (1) an increase in government spending and the consequent budget deficits tend to cause trade deficits in the Bretton Woods era; (2) in contrast, an increase in government spending tends to induce trade surpluses in the post-Bretton Woods era; and (3) with the exceptions of the 1980s and 2010s, government spending shocks cause trade deficits under a floating exchange regime. Using the open economy New Keynesian model with rule-of-thumb consumers, I find that a shift in exchange rate regimes helps in understanding empirical results (1) and (2). Moreover, slowly adjusted taxes inform our comprehension of exceptions in the 1980s, whereas zero lower bound aids our explanation of exceptions in the 2010s.
The challenges for health professionals delivering palliative care in the community during the COVID-19 pandemic: An integrative review
Authors: Frey Rosemary , Balmer Deborah 
Published: March 2023

AbstractObjectivesThe growing demand for palliative care has been accelerated due to the COVID-19 pandemic. However, providing community-based palliative care was also more difficult to do safely and faced several challenges. The goal of this integrative review was to identify, describe, and synthesize previous studies on the challenges for health professionals delivering palliative care in the community during the COVID-19 pandemic.MethodsSearches were carried out on the Ovid MEDLINE, CINAHL, PsycINFO, Social Care Online, PubMed, Embase, and Expanded Academic databases. Journals typically reporting palliative care and community health studies were also searched (Palliative Medicine, Journal of Pain and Symptom Management, and Health & Social Care in the Community). All articles were peer-reviewed and published in English between December 2019 and September 2022.ResultsDatabase and hand searches identified 1231 articles. After duplicates were removed and the exclusion criteria applied, 27 articles were included in the final review. Themes in the research findings centered on 6 interconnected categories. The challenges imposed by the pandemic (lack of resources, communication difficulties, access to education and training, and interprofessional coordination), as well as the varying levels of success of the health-care responses, impacted the well-being of health professionals and, in turn, the well-being and care of patients and families.Significance of resultsThe pandemic has provided the impetus for rethinking flexible and innovative approaches to overcome the challenges of delivering community palliative care. However, existing governmental and organizational policies require revision to improve communication and effective interprofessional collaboration, and additional resources are needed. A blended model of virtual and in-person palliative care delivery may provide the best solution to community palliative care delivery moving forward.
Okapi-EM: A napari plugin for processing and analyzing cryogenic serial focused ion beam/scanning electron microscopy images
Authors: Perdigão Luís M. A. , Ho Elaine M. L. , Cheng Zhiyuan C. , Yee Neville B.-Y. , Glen Thomas , Wu Liang , Grange Michael , Dumoux Maud , Basham Mark , Darrow Michele C. 
Published: March 2023

Abstract
An emergent volume electron microscopy technique called cryogenic serial plasma focused ion beam milling scanning electron microscopy (pFIB/SEM) can decipher complex biological structures by building a three-dimensional picture of biological samples at mesoscale resolution. This is achieved by collecting consecutive SEM images after successive rounds of FIB milling that expose a new surface after each milling step. Due to instrumental limitations, some image processing is necessary before 3D visualization and analysis of the data is possible. SEM images are affected by noise, drift, and charging effects, that can make precise 3D reconstruction of biological features difficult. This article presents Okapi-EM, an open-source napari plugin developed to process and analyze cryogenic serial pFIB/SEM images. Okapi-EM enables automated image registration of slices, evaluation of image quality metrics specific to pFIB-SEM imaging, and mitigation of charging artifacts. Implementation of Okapi-EM within the napari framework ensures that the tools are both user- and developer-friendly, through provision of a graphical user interface and access to Python programming.
Mobilization and Export of Particulate and Dissolved Solids and Organic Carbon From Contrasting Mountainous River Watersheds in California and Oregon
Authors: Goñi Miguel A. , Welch Kylie A. , Ghazi Layla , Pett‐Ridge Julie C. , Haley Brian A. 
Published: March 2023

AbstractConcentration‐discharge (C‐Q) relationships of total suspended solids (TSS), total dissolved solids (TDS), particulate organic carbon (POC), and dissolved organic carbon (DOC) were investigated in the tributaries and main‐stems of two mountainous river systems with distinct watershed characteristics (Eel and Umpqua rivers) in Northern California and central Oregon (USA). Power‐law (C = a × Qb) fits to the data showed strong transport‐limited behavior (b > 1) by TSS and POC, moderate transport limitation of DOC (b > 0.3) and chemostatic behavior (b < 0) by TDS in most streams. These contrasts led to significant compositional differences at varying discharge levels, with particle‐bound constituents becoming increasingly important (relative abundances of 50% to >90%) at high‐flow conditions. Organic carbon contents of TSS displayed marked decreases with discharge whereas they increased in TDS during high‐flow conditions. Daily and cumulative material fluxes for different coastal streams were calculated using the C‐Q relationships and showed that the delivery of transport‐limited constituents, such as TSS and POC (and DOC to a lesser degree), was closely tied to high‐discharge events and occurred primarily during the winter season. The coherence between winter fluxes and high wave‐southerly wind conditions along the coast highlights how seasonal and inter‐annual differences in fluvial discharge patterns affect the fate of land‐derived materials delivered to coastal regions.
Late‐Orogenic Evolution of the Southern European Variscan Belt Constrained by Fabric Analysis and Dating of the Camarat Granitic Complex and Coeval Felsic Dykes (Maures–Tanneron Massif, SE France)
Authors: Bolle Olivier , Corsini Michel , Diot Hervé , Laurent Oscar , Melis Raphaël 
Published: March 2023

AbstractThe Camarat Granitic Complex (CGC), emplaced in the migmatitic Internal Zone of the Maures–Tanneron Massif (MTM), SE Variscides, consists of the Gigaro granodiorite and the composite Camarat granite. U‐Pb dating of the latter gives crystallization ages of 304.5 ± 3.3 Ma (zircon date) and 303.5 ± 4.0 Ma (monazite date). Two representatives of late felsic dykes cutting across the MTM Internal Zone have 39Ar/40Ar muscovite ages of 302.43 ± 2.62 Ma and 298.11 ± 2.38. Magmatic lineations revealed by anisotropy of magnetic susceptibility (AMS) measurements and image analysis, aplite dykes, dyke‐like bodies of cordierite microgranite and joints in the Camarat granite, as well as the late dykes all have orientations consistent with subhorizontal, NNE‐SSW‐trending lineations in the migmatitic country rocks representing the stretching direction of a late‐Variscan transpression phase (D3). The CGC and the late dykes are therefore witness to a thermal event that affected the MTM between ∼305 and ∼298 Ma (late Pennsylvanian–earliest Permian times), at the end of D3 which initiated at ∼325 Ma. Grabens related to post‐Variscan, Permian rifting (D4 phase), which cut across the MTM are WNW‐ESE‐trending, indicating a NNE‐SSW direction of extension, parallel to the previous (D3) lateral horizontal flow. The present results and a comparison with AMS data published for the Corsica–Sardinia Batholith reveal that evolution in the SE Variscides from Devonian–early Carboniferous contraction to Permian extension, through late Carboniferous transpression is characterized by the persistence of a ca. N‐S stretching direction, supporting a strong horizontal, orogen‐parallel crustal flow.
Representation of Fully Three‐Dimensional Interfacial Curvature in Pore‐Network Models
Authors: Giudici Luke M. , Qaseminejad Raeini A. , Blunt Martin J. , Bijeljic Branko 
Published: March 2023

AbstractQuasi two‐dimensional approximations of interfacial curvature, present in current network models of multiphase flow in porous media, are extended to three dimensions. The new expressions for threshold capillary pressure are validated and calibrated using high‐resolution direct numerical simulations on synthetic geometries. The effects of pore‐space expansion and sagittal interface curvature on displacement are quantified, and are shown to be a key step in improving the physical accuracy of network models. Finally, the calibrated network model is used to obtain predictions for relative permeability and capillary pressure in a water‐wet Bentheimer sandstone. The predictions are compared to experimental measurements, revealing that the inclusion of three‐dimensional interfacial curvature leads to more accurate predictions.
Altered behavior, brain structure, and neurometabolites in a rat model of autism-specific maternal autoantibody exposure
Authors: Bruce Matthew R. , Couch Amalie C. M. , Grant Simone , McLellan Janna , Ku Katherine , Chang Christina , Bachman Angelica , Matson Matthew , Berman Robert F. , Maddock Richard J. , Rowland Douglas , Kim Eugene , Ponzini Matthew D. , Harvey Danielle , Taylor Sandra L. , Vernon Anthony C. , Bauman Melissa D. , Van de Water Judy 
Published: March 2023

AbstractMaternal immune dysregulation is a prenatal risk factor for autism spectrum disorder (ASD). Importantly, a clinically relevant connection exists between inflammation and metabolic stress that can result in aberrant cytokine signaling and autoimmunity. In this study we examined the potential for maternal autoantibodies (aAbs) to disrupt metabolic signaling and induce neuroanatomical changes in the brains of exposed offspring. To accomplish this, we developed a model of maternal aAb exposure in rats based on the clinical phenomenon of maternal autoantibody-related ASD (MAR-ASD). Following confirmation of aAb production in rat dams and antigen-specific immunoglobulin G (IgG) transfer to offspring, we assessed offspring behavior and brain structure longitudinally. MAR-ASD rat offspring displayed a reduction in pup ultrasonic vocalizations and a pronounced deficit in social play behavior when allowed to freely interact with a novel partner. Additionally, longitudinal in vivo structural magnetic resonance imaging (sMRI) at postnatal day 30 (PND30) and PND70, conducted in a separate cohort of animals, revealed sex-specific differences in total and regional brain volume. Treatment-specific effects by region appeared to converge on midbrain and cerebellar structures in MAR-ASD offspring. Simultaneously, in vivo 1H magnetic resonance spectroscopy (1H-MRS) data were collected to examine brain metabolite levels in the medial prefrontal cortex. Results showed that MAR-ASD offspring displayed decreased levels of choline-containing compounds and glutathione, accompanied by increased taurine compared to control animals. Overall, we found that rats exposed to MAR-ASD aAbs present with alterations in behavior, brain structure, and neurometabolites; reminiscent of findings observed in clinical ASD.
E2F1-mediated KDM4A-AS1 up-regulation promotes EMT of hepatocellular carcinoma cells by recruiting ILF3 to stabilize AURKA mRNA
Authors: Shen Hao-Ming , Zhang Di , Xiao Ping , Qu Bin , Sun Yi-Fan 
Published: March 2023

AbstractHepatocellular carcinoma (HCC) is a gastrointestinal tumor with high clinical incidence. Long non-coding RNAs (lncRNAs) play vital roles in modulating the growth and epithelial-mesenchymal transition (EMT) of HCC. However, the underlying mechanism of lncRNA KDM4A antisense RNA 1 (KDM4A-AS1) in HCC remains elusive. In our study, the role of KDM4A-AS1 in HCC was systematically investigated. The levels of KDM4A-AS1, interleukin enhancer-binding factor 3 (ILF3), Aurora kinase A (AURKA), and E2F transcription factor 1 (E2F1) were determined by RT-qPCR or western blot. ChIP and dual luciferase reporter experiments were performed to detect the binding relationship between E2F1 and KDM4A-AS1 promoter sequence. RIP and RNA-pull down confirmed the interaction of ILF3 with KDM4A-AS1/AURKA. Cellular functions were analyzed by MTT, flow cytometry, wound healing and transwell assays. IHC was performed to detect Ki67 in vivo. We found that KDM4A-AS1 was increased in HCC tissues and cells. Elevated KDM4A-AS1 level was correlated to poor prognosis of HCC. Knockdown of KDM4A-AS1 inhibited the proliferation, migration, invasion and EMT of HCC cells. ILF3 bound to KDM4A-AS1 and AURKA. KDM4A-AS1 maintained the stability of AURKA mRNA by recruiting ILF3. E2F1 transcriptionally activated KDM4A-AS1. Overexpressed KDM4A-AS1 reversed the contribution of E2F1 depletion to AURKA expression and EMT in HCC cells. KDM4A-AS1 promoted tumor formation in vivo through the PI3K/AKT pathway. These results revealed that E2F1 transcriptionally activated KDM4A-AS1 to regulate HCC progression via the PI3K/AKT pathway. E2F1 and KDM4A-AS1 may serve as good prognostic targets for HCC treatment.
Deletion of Gpatch2 does not alter Tnf expression in mice
Authors: Dalseno Destiny , Anderton Holly , Kueh Andrew , Herold Marco J , Silke John , Strasser Andreas , Bouillet Philippe 
Published: March 2023

AbstractThe cytokine TNF has essential roles in immune defence against diverse pathogens and, when its expression is deregulated, it can drive severe inflammatory disease. The control of TNF levels is therefore critical for normal functioning of the immune system and health. We have identified GPATCH2 as a putative repressor of Tnf expression acting post-transcriptionally through the TNF 3’ UTR in a CRISPR screen for novel regulators of TNF. GPATCH2 is a proposed cancer-testis antigen with roles reported in proliferation in cell lines. However, its role in vivo has not been established. We have generated Gpatch2−/− mice on a C57BL/6 background to assess the potential of GPATCH2 as a regulator of Tnf expression. Here we provide the first insights into Gpatch2−/− animals and show that loss of GPATCH2 affects neither basal Tnf expression in mice, nor Tnf expression in intraperitoneal LPS and subcutaneous SMAC-mimetic injection models of inflammation. We detected GPATCH2 protein in mouse testis and at lower levels in several other tissues, however, the morphology of the testis and these other tissues appears normal in Gpatch2−/− animals. Gpatch2−/− mice are viable, appear grossly normal, and we did not detect notable aberrations in lymphoid tissues or blood cell composition. Collectively, our results suggest no discernible role of GPATCH2 in Tnf expression, and the absence of an overt phenotype in Gpatch2−/− mice warrants further investigation of the role of GPATCH2.
Risk of death following COVID-19 vaccination or positive SARS-CoV-2 test in young people in England
Authors: Nafilyan Vahé , Bermingham Charlotte R. , Ward Isobel L. , Morgan Jasper , Zaccardi Francesco , Khunti Kamlesh , Stanborough Julie , Banerjee Amitava , Doidge James C. 
Published: March 2023

AbstractSeveral studies have reported associations between COVID-19 vaccination and risk of cardiac diseases, especially in young people; the impact on mortality, however, remains unclear. We use national, linked electronic health data in England to assess the impact of COVID-19 vaccination and positive SARS-CoV-2 tests on the risk of cardiac and all-cause mortality in young people (12 to 29 years) using a self-controlled case series design. Here, we show there is no significant increase in cardiac or all-cause mortality in the 12 weeks following COVID-19 vaccination compared to more than 12 weeks after any dose. However, we find an increase in cardiac death in women after a first dose of non mRNA vaccines. A positive SARS-CoV-2 test is associated with increased cardiac and all-cause mortality among people vaccinated or unvaccinated at time of testing.
Ultrafast 3D nanofabrication via digital holography
Authors: Ouyang Wenqi , Xu Xiayi , Lu Wanping , Zhao Ni , Han Fei , Chen Shih-Chi 
Published: March 2023

AbstractThere has been a compelling demand of fabricating high-resolution complex three-dimensional (3D) structures in nanotechnology. While two-photon lithography (TPL) largely satisfies the need since its introduction, its low writing speed and high cost make it impractical for many large-scale applications. We report a digital holography-based TPL platform that realizes parallel printing with up to 2000 individually programmable laser foci to fabricate complex 3D structures with 90 nm resolution. This effectively improves the fabrication rate to 2,000,000 voxels/sec. The promising result is enabled by the polymerization kinetics under a low-repetition-rate regenerative laser amplifier, where the smallest features are defined via a single laser pulse at 1 kHz. We have fabricated large-scale metastructures and optical devices of up to centimeter-scale to validate the predicted writing speed, resolution, and cost. The results confirm our method provides an effective solution for scaling up TPL for applications beyond laboratory prototyping.
Conserved transcription factors promote cell fate stability and restrict reprogramming potential in differentiated cells
Authors: Missinato Maria A. , Murphy Sean , Lynott Michaela , Yu Michael S. , Kervadec Anaïs , Chang Yu-Ling , Kannan Suraj , Loreti Mafalda , Lee Christopher , Amatya Prashila , Tanaka Hiroshi , Huang Chun-Teng , Puri Pier Lorenzo , Kwon Chulan , Adams Peter D. , Qian Li , Sacco Alessandra , Andersen Peter , Colas Alexandre R. 
Published: March 2023

AbstractDefining the mechanisms safeguarding cell fate identity in differentiated cells is crucial to improve 1) - our understanding of how differentiation is maintained in healthy tissues or altered in a disease state, and 2) - our ability to use cell fate reprogramming for regenerative purposes. Here, using a genome-wide transcription factor screen followed by validation steps in a variety of reprogramming assays (cardiac, neural and iPSC in fibroblasts and endothelial cells), we identified a set of four transcription factors (ATF7IP, JUNB, SP7, and ZNF207 [AJSZ]) that robustly opposes cell fate reprogramming in both lineage and cell type independent manners. Mechanistically, our integrated multi-omics approach (ChIP, ATAC and RNA-seq) revealed that AJSZ oppose cell fate reprogramming by 1) - maintaining chromatin enriched for reprogramming TF motifs in a closed state and 2) - downregulating genes required for reprogramming. Finally, KD of AJSZ in combination with MGT overexpression, significantly reduced scar size and improved heart function by 50%, as compared to MGT alone post-myocardial infarction. Collectively, our study suggests that inhibition of barrier to reprogramming mechanisms represents a promising therapeutic avenue to improve adult organ function post-injury.
An actin filament branching surveillance system regulates cell cycle progression, cytokinesis and primary ciliogenesis
Authors: Cao Muqing , Zou Xiaoxiao , Li Chaoyi , Lin Zaisheng , Wang Ni , Zou Zhongju , Ye Youqiong , Seemann Joachim , Levine Beth , Tang Zaiming , Zhong Qing 
Published: March 2023

AbstractDysfunction of cell cycle control and defects of primary ciliogenesis are two features of many cancers. Whether these events are interconnected and the driving mechanism coordinating them remains elusive. Here, we identify an actin filament branching surveillance system that alerts cells of actin branching insufficiency and regulates cell cycle progression, cytokinesis and primary ciliogenesis. We find that Oral-Facial-Digital syndrome 1 functions as a class II Nucleation promoting factor to promote Arp2/3 complex-mediated actin branching. Perturbation of actin branching promotes OFD1 degradation and inactivation via liquid-to-gel transition. Elimination of OFD1 or disruption of OFD1-Arp2/3 interaction drives proliferating, non-transformed cells into quiescence with ciliogenesis by an RB-dependent mechanism, while it leads oncogene-transformed/cancer cells to incomplete cytokinesis and irreversible mitotic catastrophe via actomyosin ring malformation. Inhibition of OFD1 leads to suppression of multiple cancer cell growth in mouse xenograft models. Thus, targeting OFD1-mediated actin filament branching surveillance system provides a direction for cancer therapy.
Single-molecule localization microscopy reveals the ultrastructural constitution of distal appendages in expanded mammalian centrioles
Authors: Chang Ting-Jui Ben , Hsu Jimmy Ching-Cheng , Yang T. Tony 
Published: March 2023

AbstractDistal appendages (DAPs) are vital in cilia formation, mediating vesicular and ciliary docking to the plasma membrane during early ciliogenesis. Although numerous DAP proteins arranging a nine-fold symmetry have been studied using superresolution microscopy analyses, the extensive ultrastructural understanding of the DAP structure developing from the centriole wall remains elusive owing to insufficient resolution. Here, we proposed a pragmatic imaging strategy for two-color single-molecule localization microscopy of expanded mammalian DAP. Importantly, our imaging workflow enables us to push the resolution limit of a light microscope well close to a molecular level, thus achieving an unprecedented mapping resolution inside intact cells. Upon this workflow, we unravel the ultra-resolved higher-order protein complexes of the DAP and its associated proteins. Intriguingly, our images show that C2CD3, microtubule triplet, MNR, CEP90, OFD1, and ODF2 jointly constitute a unique molecular configuration at the DAP base. Moreover, our finding suggests that ODF2 plays an auxiliary role in coordinating and maintaining DAP nine-fold symmetry. Together, we develop an organelle-based drift correction protocol and a two-color solution with minimum crosstalk, allowing a robust localization microscopy imaging of expanded DAP structures deep into the gel-specimen composites.