bluuebunny/crossref_metadata_2025_split huggingface dataset:
Identification of four secretory phospholipase A2s in a lepidopteran insect, Acrolepiopsis sapporensis, and their functional association with cellular immune responses
Authors: Hrithik Md Tafim Hossain , Hong Jooan , Kim Yonggyun 
Published: June 2023

BackgroundEicosanoids are a group of the oxygenated C20 polyunsaturated fatty acids and play crucial roles in mediating various insect physiological processes. Catalytic activity of phospholipase A2 (PLA2) provides an initial substrate, arachidonic acid (AA), for subsequent eicosanoid biosynthesis.ResultsThis study identified four different secretory PLA2 (As-PLA2A–As-PLA2D) genes encoded in the Asian onion moth, Acrolepiopsis sapporensis. A phylogenetic analysis indicated that As-PLA2A and As-PLA2D are clustered with Group III PLA2s while As-PLA2B and As-PLA2C are clustered with Group XII and Group X PLA2s, respectively. Expression levels of these PLA2 genes increased along with larval development, especially in the fat body. A bacterial immune challenge upregulated the basal expression levels of the four PLA2 genes, which resulted in significant increases of the PLA2 enzyme activity. The enzyme activity was susceptible to a calcium chelator or reducing agent, suggesting Ca2+ dependency and disulfide linkage required for the catalytic activities of the secretory type of PLA2s. In addition, the PLA2 activity was also susceptible to bromophenacyl bromide (BPB), a specific inhibitor to sPLA2, but not to intracellular PLA2 inhibitors. An addition of BPB to the immune challenge significantly prevented hemocyte-spreading behavior of A. sapporensis. BPB treatment also suppressed a cellular immune response measured by hemocyte nodule formation. However, the immunosuppression was significantly rescued by the AA addition. To determine the PLA2(s) responsible for the immunity, individual RNA interference (RNAi) treatments specific to each of the four PLA2s were performed. Injection of gene-specific double-stranded RNAs caused significant reductions in the transcript level in all four PLA2s. In all four PLA2s, the RNAi treatments prevented the cellular immune response even after the immune challenge.ConclusionThis study reports four secretory PLA2s encoded in A. sapporensis and their function in mediating cellular immunity.
Setbacks for gas stations in a world with regionally varying emissions factors and acceptable health risks
Authors: Hilpert Markus 
Published: June 2023

MotivationGas stations chronically release gasoline vapor to the environment that contains the carcinogen benzene. However, there is no method for estimating setbacks for a gas station depending on an acceptable excess cancer risk due to the benzene emissions, sales volume, benzene content of the emissions, and inhalation dose, without performing an air dispersion simulation for each scenario.MethodsWe developed a new modeling framework, in which only one air dispersion simulation is performed for a reference gas station. Then, a new scaling law is used to estimate cancer risks and setbacks for different gas station characteristics and exposure scenarios.ResultsOur new scaling law allows estimating cancer risk vs. distance as a function of an acceptable excess cancer risk, total benzene emission rate, and frequency of exposure. Setbacks can also be determined from this scaling law or graphically from a design chart. Calculated setbacks differ only slightly from those determined from air dispersion simulations. Different emission control technologies substantially affect calculated setbacks.ConclusionsWe developed a framework that allows policy makers to examine easily how setbacks depend on regulatable measures such as emission control and acceptable cancer risk as well as on exposure characteristics. The framework also allows incorporating a safety factor to account for increased emissions. While this study was conducted in the context of US gas stations, our framework can be applied world-wide.
Spatio-temporal variation of water requirement and meteorological impact factors of maize Shaanxi, China
Authors: Duan Jingjing , Zhao Jing , Sun Geyao , Gao Feng 
Published: June 2023

To quantitatively reveal the characteristics of the spatial and temporal distribution and the influencing factors of the maize water requirement in Shaanxi Province, the Penman-Monteith model and path analysis were used to systematically analyze the interannual variation and spatial distribution of the maize water requirement at different growth stages in three climatic zones in Shaanxi Province. And the relationship between the water requirement and meteorological factors based on daily meteorological data in Shaanxi Province for the past 60 years. The results showed that the maize water requirement during the whole growth period decreased from 1960 to 1989, with a rate of change of −2.08 mm/a, increased from 1989 to 2019, with a rate of change of 0.38 mm/a, and decreased from 1960 to 2019, with a rate of change of −0.46 mm/a. The water requirement of maize decreases from north to south during the whole reproductive period. That of seedling and male stage are more in the Guanzhong, followed by the north and the least in the south. The water requirement of maize at jointing stage increased from north to south. And the water requirement distribution is more in the north, followed by the south and the least in the Guanzhong area during the maturity period. The average temperature and the duration of the sunshine are the key factors that affect maize water requirement in Shaanxi Province, and have an increasing effect on water requirement, wind speed has an increasing effect on water requirement but is not obvious, water vapor pressure and relative humidity have an inhibiting effect on water requirement. For spring maize, the promoting effect of the duration of the sunshine on maize water requirement was gradually strengthened from north to south. The promoting effect of mean temperature and wind speed decreased gradually. The inhibitory effect of water vapor pressure and relative humidity decreases continuously. This study can provide a scientific basis for the management of irrigation quota in maize growing areas under similar cropping conditions.
Prioritization of nasal polyp-associated genes by integrating GWAS and eQTL summary data
Authors: Yoshikawa Masahiro , Asaba Kensuke , Nakayama Tomohiro 
Published: June 2023

Background: Nasal polyps (NP) are benign inflammatory growths of nasal and paranasal sinus mucosa that can substantially impair patients’ quality of life by various symptoms such as nasal obstruction, insomnia, and anosmia. NP often relapse even after surgical treatment, and the curative therapy would be challenging without understanding the underlying mechanisms. Genome wide association studies (GWASs) on NP have been conducted; however, few genes that are causally associated with NP have been identified.Methods: We aimed to prioritize NP associated genes for functional follow-up studies using the summary data-based Mendelian Randomization (SMR) and Bayesian colocalization (COLOC) methods to integrate the summary-level data of the GWAS on NP and the expression quantitative trait locus (eQTL) study in blood. We utilized the GWAS data including 5,554 NP cases and 258,553 controls with 34 genome-wide significant loci from the FinnGen consortium (data freeze 8) and the eQTL data from 31,684 participants of predominantly European ancestry from the eQTLGen consortium.Results: The SMR analysis identified several genes including TNFRSF18, CTSK, and IRF1 that were associated with NP due to not linkage but pleiotropy or causality. The COLOC analysis strongly suggested that these genes and the trait of NP were affected by shared causal variants, and thus were colocalized. An enrichment analysis by Metascape suggested that these genes might be involved in the biological process of cellular response to cytokine stimulus.Conclusion: We could prioritize several NP associated genes including TNFRSF18, CTSK, and IRF1 for follow-up functional studies in future to elucidate the underlying disease mechanisms.
Genome editing of human embryos for research purposes: Japanese lay and expert attitudes
Authors: Akatsuka Kyoko , Hatta Taichi , Sawai Tsutomu , Fujita Misao 
Published: June 2023

Background: Multiple surveys of the general public and experts on human genome editing have been conducted. However, many focused only on editing in clinical applications, with few regarding its use for basic research. Given that genome editing for research purposes is indispensable for the realization of clinical genome editing, understanding lay attitudes toward genome editing in research, particularly using human embryos, which is likely to provoke ethical concerns, is helpful for future societal discussion.Methods: An online survey was conducted with Japanese laypeople and researchers to ascertain their views regarding human genome editing for research purposes. Participants were queried about their acceptance as a function of the target of genome editing (germ cells, surplus IVF embryos, research embryos, somatic cells); then, those who answered “acceptable depending on the purpose” were asked about their acceptance in the context of specific research purposes of genome editing. Participants were also asked about their expectations and concerns regarding human genome editing.Results: Replies were obtained from 4,424 laypeople and 98 researchers. Approximately 28.2–36.9% of the laypeople exhibited strong resistance to genome editing for research purposes regardless of their applications. In contrast, 25.5% of the researchers demonstrated resistance only to genome editing in research embryos; this percentage was substantially higher than those concerning the other three targets (5.1–9.2%). Approximately 50.4–63.4% of laypeople who answered “acceptable depending on the purpose” approved germline genome editing for disease research; however, only 39.3–42.8% approved genome editing in basic research to obtain biological knowledge. In contrast, the researchers displayed a lower degree of acceptance of germline genome editing for research purposes related to chronic diseases (60.9–66.7%) than for other research purposes (73.6–90.8%). Analysis of responses concerning expectations and concerns indicated that laypeople who would not accept genome editing of human embryos did not necessarily worry about “instrumentalization of the embryo.” They also had substantially low expectations for recognized advantages of genome editing, including “advances in science” and “reduction of intractable diseases,” compared with other groups of respondents.Conclusion: The assumptions shared among experts in conventional bioethical debates and policy discussions on human genome editing are not self-evident to laypeople.
Ultrarapid and high-resolution HLA class I typing using transposase-based nanopore sequencing applied in pharmacogenetic testing
Authors: Anukul Nampeung , Jenjaroenpun Piroon , Sirikul Chonticha , Wankaew Natnicha , Nimsamer Pattaraporn , Roothumnong Ekkapong , Pithukpakorn Manop , Leetrakool Nipapan , Wongsurawat Thidathip 
Published: June 2023

Nanopore sequencing has been examined as a method for rapid and high-resolution human leukocyte antigen (HLA) typing in recent years. We aimed to apply ultrarapid nanopore-based HLA typing for HLA class I alleles associated with drug hypersensitivity, including HLA-A*31:01, HLA-B*15:02, and HLA-C*08:01. Most studies have used the Oxford Nanopore Ligation Sequencing kit for HLA typing, which requires several enzymatic reactions and remains relatively expensive, even when the samples are multiplexed. Here, we used the Oxford Nanopore Rapid Barcoding kit, which is transposase-based, with library preparation taking less than 1 h of hands-on time and requiring minimal reagents. Twenty DNA samples were genotyped for HLA-A, -B, and -C; 11 samples were from individuals of different ethnicity and nine were from Thai individuals. Two primer sets, a commercial set and a published set, were used to amplify the HLA-A, -B, and -C genes. HLA-typing tools that used different algorithms were applied and compared. We found that without using several third-party reagents, the transposase-based method reduced the hands-on time from approximately 9 h to 4 h, making this a viable approach for obtaining same-day results from 2 to 24 samples. However, an imbalance in the PCR amplification of different haplotypes could affect the accuracy of typing results. This work demonstrates the ability of transposase-based sequencing to report 3-field HLA alleles and its potential for race- and population-independent testing at considerably decreased time and cost.
Association of the rs1990760, rs3747517, and rs10930046 polymorphisms in the IFIH1 gene with susceptibility to autoimmune diseases: a meta-analysis
Authors: Xiao Zilin , Luo Shuoming , Zhou Yuemin , Pang Haipeng , Yin Wenfeng , Qin Jiabi , Xie Zhiguo , Zhou Zhiguang 
Published: June 2023

ObjectiveInterferon induced with helicase C domain 1 (IFIH1) single-nucleotide polymorphisms (SNP) rs1990760, rs3747517, and rs10930046 have been shown to be closely related to the risk of autoimmune diseases. The aim of this study was firstly to examine the association of the rs1990760 with type 1 diabetes (T1D) in a Chinese population. Secondly, to assess the association of SNP rs1990760, rs3747517, and rs10930046 with autoimmune diseases susceptibility.MethodsA total of 1,273 T1D patients and 1,010 healthy control subjects in a Chinese population were enrolled in this case-control study. Subsequently, we performed a meta-analysis on the association of the SNP rs1990760, rs3747517, and rs10930046 in the IFIH1 gene with susceptibility to autoimmune diseases. The random and fixed genetic effects models were used to evaluate the association and the effect sizes, including odds ratios (OR) and 95% confidence intervals (CI). Stratification analyses based on ethnicity and the type of autoimmune diseases were performed.ResultsIFIH1 SNP rs1990760 was not associated with a significant risk of T1D in the Chinese population in the case-control study. A total of 35 studies including 70,966 patients and 124,509 controls were identified and included in the meta-analysis. The results displayed significant associations between IFIH1 rs1990760 A allele and rs3747517 C allele and autoimmune diseases risk (OR=1.09, 95% CI: 1.01~1.17; OR=1.24, 95% CI: 1.15~1.25, respectively). Stratified analysis indicated a significant association rs1990760 and rs3747517 with autoimmune diseases risk in the Caucasian population (OR=1.11, 95% CI: 1.02~1.20, OR=1.29, 95% CI: 1.18~1.41, respectively).ConclusionsThis study revealed no association between IFIH1 SNP rs1990760 and T1D in Chinese. Furthermore, the meta-analysis indicated that rs1990760 and rs3747517 polymorphisms, confer susceptibility to autoimmune diseases, especially in the Caucasian population.
Distinct subtypes of endometriosis identified based on stromal-immune microenvironment and gene expression: implications for hormone therapy
Authors: Wang Yuning , Chen Yun , Xiao Yinping , Ruan Jingyao , Tian Qi , Cheng Qi , Chang Kaikai , Yi Xiaofang 
Published: June 2023

BackgroundEndometriosis (EMs) is a chronic inflammatory condition that is highly heterogeneous. Current clinical staging fails to accurately predict drug responses and prognosis. In this study, we aimed to reveal the heterogeneity of ectopic lesions and investigate the possible underlying mechanisms using transcriptomic data and clinical information.MethodsThe EMs microarray dataset GSE141549 was obtained from the Gene Expression Omnibus database. Unsupervised hierarchical clustering was performed to identify EMs subtypes, which was followed by the functional enrichment analysis and estimation of immune infiltrates. Subtype-associated gene signatures were identified and further validated in other independent datasets, including GSE25628, E-MTAB-694, and GSE23339. Additionally, tissue microarrays (TMAs) were generated from premenopausal patients with EMs to investigate the potential clinical implications of the two identified subtypes.ResultsThe unsupervised clustering analysis revealed that ectopic EMs lesions can be classified into two distinct subtypes: stroma-enriched (S1) and immune-enriched (S2). The functional analysis revealed that S1 correlated with fibroblast activation and extracellular matrix remodeling in the ectopic milieu, whereas S2 was characterized by the upregulation of immune pathways and a higher positive correlation with the immunotherapy response. Moreover, we identified a subtype signature composed of FHL1 and SORBS1, and constructed a subtype diagnostic model. Based on the cohort data from the TMAs, we found that S2 was strongly associated with the failure of/intolerance to hormone therapy.ConclusionsThis study identified two distinct subtypes that are varyingly associated with hormone resistance, stroma-immunity, and molecular features, thereby highlighting the importance of this stromal-immune heterogeneity in identifying EMs subtypes and providing novel insights into future personalized hormone-free therapy in EMs.
Planning sentence production in aphasia: evidence from structural priming and eye-tracking
Authors: van Boxtel Willem S. , Cox Briana N. , Keen Austin , Lee Jiyeon 
Published: June 2023

BackgroundGrammatical encoding is impaired in many persons with aphasia (PWA), resulting in deficits in sentence production accuracies and underlying planning processes. However, relatively little is known on how these grammatical encoding deficits can be mediated in PWA. This study aimed to facilitate off-line (accuracy) and real-time (eye fixations) encoding of passive sentences through implicit structural priming, a tendency to better process a current sentence because of its grammatical similarity to a previously experienced (prime) sentence.MethodSixteen PWA and Sixteen age-matched controls completed an eyetracking-while-speaking task, where they described a target transitive picture preceded by a comprehension prime involving either an active or passive form. We measured immediate and cumulative priming effects on proportions of passives produced for the target pictures and proportions of eye fixations made to the theme actor in the target scene before speech onset of the sentence production.Results and conclusionBoth PWA and controls produced cumulatively more passives as the experiment progressed despite an absence of immediate priming effects in PWA. Both groups also showed cumulative changes in the pre-speech eye fixations associated with passive productions, with this cumulative priming effect greater for the PWA group. These findings suggest that structural priming results in gradual adaptation of the grammatical encoding processes of PWA and that structural priming may be used as a treatment component for improving grammatical deficits in aphasia.
Influencing factors associated with high myopia in Chinese college students
Authors: Zhang Weiran , Hou Xiaoyun , Li Chang , Wang Sennan , Liu Nianen , Zhang Yan , Li Zhiqing 
Published: June 2023

BackgroundHigh myopia (HM) may elicit irreversible pathological changes in the fundus and severely impair visual quality, thereby becoming a major public health issue in China. However, the influencing factors associated with HM remain unknown in Chinese college students, whose visual quality is crucial to country development.MethodsThis is a cross-sectional observational study. Two thousand three hundred and fifteen undergraduate and graduate students were initially recruited from various majors in 3 universities in Tianjin, China. Under the principle of voluntary participation and informed consent, simple random sampling was conducted in the recruited subjects while maintaining balanced number of subjects from each major. After screening with inclusion and exclusion criteria, 96 undergraduate and graduate students (186 eyes) were finally included and divided into non-HM and HM groups. The eyes of subjects were examined by optical coherence tomography angiography (OCTA) for vessel density and structure thickness at the macula and optic disc, and the subjects were surveyed by an itemized questionnaire on lifestyles and study habits.ResultsThe OCTA and questionnaire results revealed 10 factors, including hemodynamic and anatomic parameters and lifestyle metrics, with statistical significance between the non-HM and HM groups. Receiver operating characteristic curve analysis showed that vessel density of the inner retina at the macula, vessel density of the radial peripapillary capillary at the optic disc, smartphone usage time, continuous near work time, and sleeping after midnight had superior values of area under the curve (AUC &gt; 0.700). Therefore, these 5 factors were selected for univariant and multivariant logistic regression analyses. A prediction model comprising the 5 influencing factors had an AUC of 0.940 and 95% CI of 0.908–0.972.ConclusionThis study for the first time identified the vessel density of the inner retina at the macula, the vessel density of the radial peripapillary capillary at the optic disc, smartphone usage time, continuous near work time, and sleeping after midnight as influencing factors associated with HM in Chinese college students. A prediction model comprising the 5 influencing factors was proposed for calculating likelihood of a Chinese college student developing HM, based on which lifestyle improvement and medical intervention might be recommended.
The impact of loneliness on quality of life in people with Parkinson’s disease: results from the Survey of Health, Ageing and Retirement in Europe
Authors: Prell Tino , Schönenberg Aline , Heimrich Konstantin G. 
Published: June 2023

BackgroundLoneliness is a growing issue for public health in an aging society. However, there is a lack of research on loneliness in people with Parkinson’s disease (PwPD).MethodsWe analyzed cross-sectional and longitudinal data from wave 5 (N = 559 PwPD) and 6 (N = 442 PwPD) from the Survey of Health, Ageing and Retirement in Europe (SHARE). Loneliness was assessed using the three-item version of the Revised UCLA Loneliness Scale. Descriptive statistics, group comparisons, multiple linear regressions, and generalized estimating equation analysis were performed to explore loneliness prevalence, its relationship with other factors, and its impact on Quality of Life (QoL) in PwPD.ResultsDepending on the used cut-off, the prevalence of loneliness in PwPD ranged from 24.1 to 53.8%. These prevalences were higher compared to people without PD. Loneliness was mainly linked to decreased functional abilities, weaker grip strength, more symptoms of depression, and country of residence. Loneliness was also associated with current QoL and predicts future QoL in PwPD, highlighting its impact on well-being.ConclusionAddressing loneliness could potentially improve QoL for PwPD, making it a modifiable risk factor that clinicians and policy-makers should consider.