bluuebunny/crossref_metadata_2025_split huggingface dataset:
Multifaceted role of redox pattern in the tumor immune microenvironment regarding autophagy and apoptosis
Authors: Ren Yuqing , Wang Ruizhi , Weng Siyuan , Xu Hui , Zhang Yuyuan , Chen Shuang , Liu Shutong , Ba Yuhao , Zhou Zhaokai , Luo Peng , Cheng Quan , Dang Qin , Liu Zaoqu , Han Xinwei 
Published: August 2023

AbstractThe reversible oxidation-reduction homeostasis mechanism functions as a specific signal transduction system, eliciting related physiological responses. Disruptions to redox homeostasis can have negative consequences, including the potential for cancer development and progression, which are closely linked to a series of redox processes, such as adjustment of reactive oxygen species (ROS) levels and species, changes in antioxidant capacity, and differential effects of ROS on downstream cell fate and immune capacity. The tumor microenvironment (TME) exhibits a complex interplay between immunity and regulatory cell death, especially autophagy and apoptosis, which is crucially regulated by ROS. The present study aims to investigate the mechanism by which multi-source ROS affects apoptosis, autophagy, and the anti-tumor immune response in the TME and the mutual crosstalk between these three processes. Given the intricate role of ROS in controlling cell fate and immunity, we will further examine the relationship between traditional cancer therapy and ROS. It is worth noting that we will discuss some potential ROS-related treatment options for further future studies.
Air pollution induces pyroptosis of human monocytes through activation of inflammasomes and Caspase-3-dependent pathways
Authors: Gałuszka-Bulaga Adrianna , Tkacz Karolina , Węglarczyk Kazimierz , Siedlar Maciej , Baran Jarek 
Published: August 2023

AbstractAccording to the World Health Organization (WHO), air pollution is one of the most serious threats for our planet. Despite a growing public awareness of the harmful effects of air pollution on human health, the specific influence of particulate matter (PM) on human immune cells remains poorly understood. In this study, we investigated the effect of PM on peripheral blood monocytes in vitro. Monocytes from healthy donors (HD) were exposed to two types of PM: NIST (SRM 1648a, standard urban particulate matter from the US National Institute for Standards and Technology) and LAP (SRM 1648a with the organic fraction removed). The exposure to PM-induced mitochondrial ROS production followed by the decrease of mitochondrial membrane potential and activation of apoptotic protease activating factor 1 (Apaf-1), Caspase-9, and Caspase-3, leading to the cleavage of Gasdermin E (GSDME), and initiation of pyroptosis. Further analysis showed a simultaneous PM-dependent activation of inflammasomes, including NLRP3 (nucleotide-binding oligomerization domain-like receptor pyrin domain containing 3) and Caspase-1, followed by cleavage of Gasdermin D (GSDMD) and secretion of IL-1β. These observations suggest that PM-treated monocytes die by pyroptosis activated by two parallel signaling pathways, related to the inorganic and organic PM components. The release of IL-1β and expression of danger-associated molecular patterns (DAMPs) by pyroptotic cells further activated the remnant viable monocytes to produce inflammatory cytokines (TNF-α, IL-6, IL-8) and protected them from death induced by the second challenge with PM.In summary, our report shows that PM exposure significantly impacts monocyte function and induces their death by pyroptosis. Our observations indicate that the composition of PM plays a crucial role in this process—the inorganic fraction of PM is responsible for the induction of the Caspase-3-dependent pyroptotic pathway. At the same time, the canonical inflammasome path is activated by the organic components of PM, including LPS (Lipopolysaccharide/endotoxin).
Graphical Abstract
Education mediates the relationship of parental socioeconomic status with subjective adult oral health
Authors: Haider Faten , Bernabé Eduardo , Delgado-Angulo Elsa Karina 
Published: August 2023

Abstract
Background
Evidence shows that both socioeconomic status (SES) during childhood and education are associated with adult oral health. However, whether the range of opportunities families have regarding their children’s education mediate the effect of childhood disadvantage on oral health later in life remains unknown. The aim of this study was to evaluate the mediating role of education in the association between parental SES and subjective oral health status in middle adulthood.

Methods
Data from 6703 members of the British Cohort Study 1970 were analyzed. Parental SES was measured using the 7-class National Statistics Socio-Economic Classification (NS-SEC) at age 10 years. Five measures of education (type of high school, highest qualification, age left full-time education, status of institution and field of study) were obtained from ages 16 and 42 years. Subjective oral health was measured with a single global item at age 46 years. Causal mediation analysis was performed, using a weighting-based approach, to evaluate how much of the effect of parental SES on subjective oral health was mediated by the measures of education separately and jointly.

Results
Overall, 23.6% of individuals reported poor oral health. Parental SES was associated with every measure of education, and they were also associated with subjective oral health in regression models adjusted for confounders. The effect of parental SES on subjective oral health was partially mediated by each measure of education, with a proportion mediated of 53.2% for the institution status, 46.5% for the field of study, 42.8% for the school type, 38.9% for the highest qualification earned and 38.4% for the age when full-time education was discontinued. The proportion of the effect of parental SES on subjective oral health jointly mediated by all measures of education was 81.1%.

Conclusion
This study found a substantial mediating role of education in the association between parental SES and subjective oral health in middle adulthood.
Restenosis after excimer laser coronary atherectomy and drug-coated balloon dilation in Takayasu’s arteritis: a case report and review of the literature
Authors: Liang Shichu , Yang Jinming , Ma Min , Zhou Minggang , Liu Zhiyue , Huang He , He Yong 
Published: August 2023

AbstractTakayasu’s arteritis (TAK) is a rare chronic granulomatous arteritis that mainly affects the aorta and its major branches. Coronary artery (CA) involvement can be observed in 10–25% of TAK patients. We report a 21-year-old young female who was previously diagnosed with TAK and severe left main coronary artery (LMCA) stenosis and underwent numerous percutaneous coronary interventions (PCIs) in our hospital due to in-stent restenosis (ISR). This time, an excimer laser coronary atherectomy (ELCA) and drug-coated balloon (DCB) dilation was taken at the LMCA for the ISR. The blood flow was smooth after the operation, and she was symptom-free after discharge. Unfortunately, 5 months later, severe intimal hyperplasia was still seen in the stent of LMCA and left anterior descending (LAD) coronary artery. A coronary artery bypass graft surgery (CABG) was performed, and she has been symptom-free ever since. ELCA plus DCB is one of the novel ways we first reported. However, ensuring long-term inflammation control is equally important to restore blood flow. The combination of revascularization and anti-inflammation/immunosuppression is recommended to improve the outcomes of TAK patients with CA involvements.
Exploring the landscape of Babesia bovis vaccines: progress, challenges, and opportunities
Authors: Santos John Harvey M. , Siddle Hannah V. , Raza Ali , Stanisic Danielle I. , Good Michael F. , Tabor Ala E. 
Published: August 2023

AbstractBovine babesiosis, caused by different Babesia spp. such as B. bovis, B. bigemina, B. divergens, and B. major, is a global disease that poses a serious threat to livestock production. Babesia bovis infections are associated with severe disease and increased mortality in adult cattle, making it the most virulent agent of bovine babesiosis. Babesia bovis parasites undergo asexual reproduction within bovine red blood cells, followed by sexual reproduction within their tick vectors, which transmit the parasite transovarially. Current control methods, including therapeutic drugs (i.e., imidocarb) have been found to lead to drug resistance. Moreover, changing environmental factors add complexity to efficient parasite control. Understanding the fundamental biology, host immune responses, and host–parasite interactions of Babesia parasites is critical for developing next-generation vaccines to control acute disease and parasite transmission. This systematic review analyzed available research papers on vaccine development and the associated immune responses to B. bovis. We compiled and consolidated the reported vaccine strategies, considering the study design and rationale of each study, to provide a systematic review of knowledge and insights for further research. Thirteen studies published since 2014 (inclusive) represented various vaccine strategies developed against B. bovis such as subunit, live attenuated, and viral vector vaccines. Such strategies incorporated B. bovis proteins or whole live parasites with the latter providing the most effective prophylaxis against bovine babesiosis. Incorporating novel research approaches, such as "omics" will enhance our understanding of parasite vulnerabilities.
Graphical Abstract
Enhanced activity of the left precuneus as a predictor of visuospatial dysfunction correlates with disease activity in rheumatoid arthritis
Authors: Zheng Yanmin , Xie Lei , Huang Zikai , Peng Jianhua , Huang Shuxin , Guo Ruiwei , Huang Jinzhuang , Lin Zhirong , Zhuang Zelin , Yin Jingjing , Hou Zhiduo , Ma Shuhua 
Published: August 2023

Abstract
Objective
To identify the potential diagnostic biomarkers of rheumatoid arthritis (RA) and assess the relation between visuospatial dysfunction and disease activity in RA patients using mental rotation task (MRT)-based functional magnetic resonance imaging (fMRI).

Methods
A total of 27 RA patients (11 in remission, 16 in active) and 27 well-matched controls were enrolled. The visuospatial function of the subjects was measured by MRT. Brain activity data were collected using blood oxygen level dependent fMRI technique under MRT. Disease activity score 28 (DAS28) was used to evaluate the disease severity of RA patients. An analysis of the correlations between abnormal visuospatial-related brain regions, MRT performance, and DAS28 was conducted.

Results
RA patients performed worse on MRT than controls. Compared to the control group, RA patients showed enhanced activation in the left precuneus, left superior frontal gyrus and right cingulate gyrus during the rotation task, with left hemisphere dominance. RA patients in active showed enhanced activation in the left precuneus, left middle frontal gyrus and right cingulate gyrus compared to the patients in remission. The left precuneus activation was negatively correlated with MRT accuracy (r = −0.621, p = 0.01) and positively correlated with DAS28 (r = 0.710, p = 0.002), and MRT accuracy was negatively correlated with DAS28 in RA patients (r = −0.702, p = 0.002).

Conclusion
Enhanced activation of the left precuneus in RA patients affects visuospatial function and is closely related to disease activity. These changes may provide a valuable diagnostic neuroimaging biomarker of RA.
Considerable decreased fruits and snack consumption in Iran population during COVID-19 lockdown: a cross-sectional web-based survey National Food and Nutrition Surveillance
Authors: Amini Maryam , Doustmohammadian Azam , Nikooyeh Bahareh , Clark Cain C. T. , Rabiei Samira , Ghodsi Delaram , Rasekhi Hamid , Abdollahi Zahra , Minaie Mina , Sadeghi Ghotbabadi Farzaneh , Neyestani Tirang R. 
Published: August 2023

AbstractBackgroundInadequate fruits and vegetables intake is associated with several diseases. This study aimed to explore changes in fruits and snack consumption during the COVID-19-related lockdown in Iran.MethodsAn online cross-sectional survey among Iranian households was conducted from the 4th to 25th April 2020, during the COVID-19 lockdown. Data were collected about any changes in frequency consumption of the fruits and snacks and the reasons for the changes. Ordinal logistic regression was performed to examine which factors contributed to changes in consumption. To account for the complex sampling design and allow inferences valid for the population, sampling weights were used in all analyses.ResultsA total of 21,290 households took part in the survey. During the epidemic, consumption of fruits and snacks decreased by 35% and 44%, respectively. Among the households whose fruit intake was decreased, about 21% fully omitted it from their food baskets. Residing in rural areas, having more than six members in the household, income loss, and residing in deprived provinces were directly associated with decreased consumption of fruits and snacks. Being a female-headed household was positively associated, and having persons with COVID-19 in the households was inversely associated, with decreased consumption of fruits. The main reason for reducing fruit and snack consumption was income loss.ConclusionsA significant decline in fruit and snack consumption because of COVID-19 lockdown was seen among the Iranian households. It is likely that these changes in dietary habits will become longstanding with consequent adverse health outcomes.
Eliciting beekeepers’ preferences for the small hive beetle control policy in Italy: a contingent valuation survey approach
Authors: Salvioni Cristina , Cerroni Simone 
Published: August 2023

AbstractAethina tumida, commonly known as the small hive beetle (SHB), is a parasite of social bee colonies. In 2014, when the beetle was first detected in the Italian Region of Calabria, the Italian Ministry of Health started an SHB control strategy. Over time, dissatisfaction with the control measures has grown among beekeepers and eroded compliance with the reporting obligations. Our study analyzes Southern Italian beekeepers’ preferences toward alternative SHB control policy. We use a contingent valuation survey to elicit beekeepers' preferences for five alternative control strategies. We find the ex-post biosecurity measure in place reflects in the lowest reporting rate. Our results suggest that implementing the destruction of infested hives only (selective destruction) can be a first, effective step toward enhancing compliance with the reporting obligations. Our findings also suggest that training and extension can be a winning strategy to improve beekeepers’ collaboration with the passive SHB surveillance system.
Effects of different starch structures on energy metabolism in pigs
Authors: Gao Xiaoqian , Yu Bing , Yu Jie , Mao Xiangbing , Huang Zhiqing , Luo Yuheng , Luo Junqiu , Zheng Ping , Yan Hui , He Jun , Chen Daiwen 
Published: August 2023

Abstract
Background
Starch is a major component of carbohydrates and a major energy source for monogastric animals. Starch is composed of amylose and amylopectin and has different physiological functions due to its different structure. It has been shown that the energy supply efficiency of amylose is lower than that of amylopectin. However, there are few studies on the effect of starch structure on the available energy of pigs. The purpose of this study was to measure the effect of different structures of starch in the diet on the net energy (NE) of pigs using a comparative slaughter method and to establish a prediction equation to estimate the NE of starch with different structures. Fifty-six barrows (initial BW 10.18 ± 0.11 kg) were used, and they were housed and fed individually. Pigs were divided into 7 treatments, with 8 replicates for each treatment and 1 pig for each replicate. One of the treatments was randomly selected as the initial slaughter group (ISG). Pigs in the remaining treatments were assigned to 6 diets, fed with basic diet and semi-pure diets with amylose/amylopectin ratio (AR) of 3.09, 1.47, 0.25, 0.15 and 0.12, respectively. The experiment lasted for 28 d.

Results
Results showed that compared with the high amylose (AM) groups (AR 3.09 and 1.47), the high amylopectin (AP) group (AR 0.15) significantly increased the final BW, average daily weight gain and average daily feed intake of pigs (P < 0.05), but the F:G of the AM group was lower (P < 0.01). In addition, AR 0.15 and 0.12 groups have higher (P < 0.01) nutrient digestibility of dry matter, crude protein, gross energy and crude ash. Meanwhile, compared with other groups, AR 0.15 group has a higher (P < 0.05) NE intake and energy retention (RE). The regressive equation for predicting with starch structures was established as RE = 1,235.243 − 48.298AM/AP (R2 = 0.657, P = 0.05).

Conclusions
In conclusion, NE intake and RE of pigs augmented with the increase of dietary amylopectin content, indicating that diets high in amylopectin were more conducive to promoting the growth of pigs in the late conservation period.
Infection of sheep by Echinococcus multilocularis in Gansu, China: evidence from mitochondrial and nuclear DNA analysis
Authors: Shumuye Nigus Abebe , Li Li , Li Wen-Hui , Zhang Nian-Zhang , Wu Yan-Tao , Wu Yao-Dong , Tian Wen-Jun , Zhang Lin-Sheng , Nian Xiao-Feng , Dai Guo-Dong , Chen Wei-Gang , Gao Sheng-Zhi , Tian Xue-Qi , Liu Jun-Shi , Li Bin , Kebede Nigatu , Fu Bao-Quan , Yan Hong-Bin , Jia Wan-Zhong 
Published: August 2023

Abstract
Background
In the normal life cycle of the parasite (Echinococcus multilocularis) that causes alveolar echinococcosis, domestic and wild carnivores act as definitive hosts, and rodents act as intermediate hosts. The presented study contributes to the research on the distribution and transmission pattern of E. multilocularis in China having identified sheep as an unusual intermediate host taking part in the domestic transmission of alveolar echinococcosis in Gansu Province, China.

Methods
From 2020 to 2021, nine whitish different cyst-like were collected from the liver of sheep in Gansu Province for examination. A near complete mitochondrial (mt) genome and selected nuclear genes were amplified from the cyst-like lesion for identification. To confirm the status of the specimen, comparative analysis with reference sequences, phylogenetic analysis, and network analysis were performed.

Results
The isolates displayed ≥ 98.87% similarity to E. multilocularis NADH dehydrogenase sub-unit 1 (nad1) (894 bp) reference sequences deposited in GenBank. Furthermore, amplification of the nad4 and nad2 genes also confirmed all nine samples as E. multilocularis with > 99.30% similarity. Additionally, three nuclear genes, pepck (1545 bp), elp-exons VII and VIII (566 bp), and elp-exon IX (256 bp), were successfully amplified and sequenced for one of the isolates with 98.42% similarity, confirming the isolates were correctly identified as E. multilocularis. Network analysis also correctly placed the isolates with other E. multilocularis.

Conclusions
As a result of the discovery of E. multilocularis in an unusual intermediate host, which is considered to have the highest zoonotic potential, the result clearly demonstrated the necessity for expanded surveillance in the area.

Graphical Abstract
Validation of a photogrammetric approach for the objective study of early bowed instruments
Authors: Beghin Philémon , Ceulemans Anne-Emmanuelle , Fisette Paul , Glineur François 
Published: August 2023

Abstract
Some early violins have been reduced during their history to fit imposed morphological standards, while more recent ones have been built directly to these standards. We propose an objective photogrammetric approach to differentiate between a reduced and an unreduced instrument, whereby a three-dimensional mesh is studied geometrically by examining 2D slices. Our contribution is twofold. First, we validate the quality of the photogrammetric mesh through a comparison with reference images obtained by medical imaging, and conclude that a sub-millimetre accuracy is achieved. Then, we show how quantitative and qualitative features such as contour lines, channel of minima and a measure of asymmetry between the upper and lower surfaces of a violin can be automatically extracted from the validated photogrammetric meshes, allowing to successfully highlight differences between instruments.

Graphical Abstract
Liquid chromatography-tandem mass spectrometry for pharmacokinetics evaluation of AZD5305, a selective PARP1 inhibitor, in mice
Authors: Lee Gi Ju , Kim Jin Woo , Choi Hae-In , Choi Jin Young , Cho Kwan Hyung , Koo Tae-Sung 
Published: August 2023

AbstractPoly (ADP-ribose) polymerase (PARP) inhibitors that are effective against ovarian and breast cancers with breast cancer susceptibility gene (BRCA) mutations have undesirable side effects, such as hematological toxicity. AZD5305, a selective PARP1 inhibitor currently in Phase 1/2 clinical trials, may avoid the side effects caused by PARP2. However, the in vivo pharmacokinetic characteristics of AZD5305 and its bioanalytical methods are unknown. Therefore, a method based on liquid chromatography with tandem mass spectroscopy (LC–MS/MS) was developed and validated to quantify AZD5305 in plasma of mice. Optimal chromatographic separation in terms of peak intensity and symmetry was acquired using a 4-µm Polar-RP 80 Å (2.0 × 150 mm) column with ammonium acetate (5 mM) in distilled water–acetonitrile (50:50, v/v). The retention times of AZD5305 and internal standard (IS; olaparib) were 1.82 min and 1.99 min, respectively. Detection was carried out via triple quadrupole mass spectrometry in positive ion mode employing multiple reaction monitoring transitions at m/z 407.0 → 376.0 for AZD5305 and m/z 435.0 → 281.2 for the IS. The LC–MS/MS method was linear in the range 1–1000 ng/mL with a correlation coefficient ≥ 0.990 and showed acceptable values of major parameters including accuracy, precision, and recovery. Additionally, AZD5305 showed high stability under various conditions. The in vivo and in vitro pharmacokinetics of AZD5305 were successfully characterized by employing the validated LC–MS/MS method. A high level of drug exposure and linear pharmacokinetics were observed after intravenous (IV) bolus and oral administration (PO) of AZD5305 at 0.1–1 mg/kg and 0.1–3 mg/kg, respectively. The bioavailability was close to 100%, and the metabolic stability of AZD5305 in hepatic microsomes of mice and humans was very high. These results may contribute to the improvement of PARP inhibitors that are used to treat malignancies originating from BRCA mutations.
On the genesis of multi-peak vertical structure of quasi-two-day waves in the mesosphere–lower thermosphere
Authors: Prijith S. S. , Kishore Kumar K. 
Published: August 2023

AbstractThe study presents multi-peak vertical structure of westward propagating quasi-two-day wave with wave-number 3 (W3) and its formation mechanisms in the mesosphere–lower thermosphere, using two decades of multi-satellite measurements, for the first time. W3 in austral summer shows a multi peak vertical structure, with two prominent peaks having amplitudes of 11.24 ± 2.2 K and 8.66 ± 1.1 K at 108 km (M1) and 84 km (M2), respectively. Genesis of this multi-peak structure, which is not explored in details hitherto, is investigated. While weak eastward/westward winds favour vertical growth of W3 in the regions of M1 and M2, strong eastward winds weaken W3 in the region between M1 and M2. These changes in zonal winds are modulated by the altitude variations in meridional temperature gradient and gravity wave filtering. Thus, vertically alternating changes in wind and meridional temperature gradient are the potential drivers for the multi-peak vertical structure of W3, which will be of importance for atmosphere–ionosphere coupling processes.
Graphical Abstract