bluuebunny/crossref_metadata_2025_split huggingface dataset:
The microbial communities and metabolic profiles of follicular fluid in patients with premature ovarian insufficiency
Authors: Wang Wei , Shu Mingming , Li Jianhua , Wang Qihang , Zhang Wendan , Wang Ye , Guo Yiming , Cheng Yanbin , Jiang Honghong , Song Chunlan , Liu Yuan , Shang Wei 
Published: January 2025

IntroductionPremature ovarian insufficiency (POI) is a condition characterized by ovarian dysfunction occurring before the age of 40, and its etiology is multifactorial, including genetic, immunological, infectious, environmental, and iatrogenic factors, with over half of the cases remaining unexplained. Whether the microbial communities and metabolites in follicular fluid, which is the direct microenvironment for oocyte survival, are related to POI has not been reported.MethodsIn this study, Follicular fluid samples of 26 patients with POI and 27 controls with a normal ovarian reserve were collected and analyzed using 16S rDNA sequencing and untargeted metabolomics. Conjoint analysis was performed to identify key microbial communities and metabolites that might be involved in POI.ResultsPatients with POI exhibited significant alterations in microbial richness and diversity and metabolic profile in their follicular fluid. The downregulation of ABC transporters and upregulation of the citrate cycle (TCA cycle) might be critical for the development and progression of POI. G-Rhodopseudomonas and g-Caulobacter were identified as key microbial genera, while L-aspartic acid, citrate, isoleucine, and cytidine were identified as key metabolites.DiscussionThese findings offer novel insights into the pathogenesis of POI and might pave the way for improved clinical outcomes for individuals with POI.
Family planning and preimplantation testing: family experiences in congenital adrenal hyperplasia
Authors: Sandy Jessica L. , Betts Grant , Harper Jessica L. , Nevin Suzanne M. , Deans Rebecca , Neville Kristen A. 
Published: January 2025

IntroductionPre-implantation testing (PGT) is often suggested by healthcare professionals (HCP) to parents of children with congenital adrenal hyperplasia (CAH) considering subsequent children. Despite this, some families choose to conceive naturally without genetic testing and intervention. The aims of this study were to explore fertility choices of couples with a child with CAH and the decision making process and perceptions behind these choices, and to explore the families’ lived experiences with CAH and the couples’ subsequent fertility journey. A better healthcare professional understanding of these experiences may subsequently help guide clinicians to better manage and support families of children with CAH and other autosomal recessive conditions.MethodsAll parents of current children of a tertiary service in 2020 with 21-hydroxylase deficient CAH who made an active decision regarding family planning after diagnosis of their index child were invited to participate in a semi-structured interview. Thematic analysis was performed using an inductive, semantic approach.ResultsThirty families (34 children) were identified. Fourteen considered subsequent children and had directed genetic counselling. Eight decided to have additional children of whom seven agreed to participate. Thematic analysis identified six key domains. Psychological impact surrounding the CAH diagnosis was long-lasting, causing symptoms of trauma including depression and anxiety, and influencing a couple’s choice to pursue PGT to avoid having another affected child. The perception of the index child having a mild phenotype, and fear of a more severe phenotype, often supported this decision. Conversely, lived experience of CAH and low day-to-day impact, along with a negative experience of PGT, with a greater than anticipated financial, physical, and emotional toll, led some families to subsequently consider natural conception. The role of the healthcare professional (HCP) was important in the CAH and family planning journeys. A perceived poor understanding of CAH, overstating its potential seriousness, contributed to distress. Parents reported feeling pressured to undergo PGT. Peer-support had a universally positive impact on family experience.Discussion/conclusionsThis study highlights the complex and dynamic nature of fertility decision-making, and the importance of HCP empathy and open-mindedness. Education of HCP and encouraging peer support may improve the CAH and fertility journey for families.
Investigating the asymmetric impact of tourism, green fiscal policy, and fintech on environmental emissions and coastal water quality: an empirical study using the method of moments quantile regression
Authors: Yang Mengqi , Tang Xing 
Published: January 2025

IntroductionThe factors that significantly and negatively impact carbon dioxide (CO2) emissions and coastal water quality (CWQ) must be continuously monitored and thoroughly evaluated. Among these, tourism (TR) volume stands out as one of the primary contributors to such effects. In contrast, green fiscal policy (GFP) and fintech (FT) can be considered proactive and modern efforts contributing to the improvement of these environmental indicators. Exploring whether the impacts of these factors exhibit uniformity across quantiles will greatly benefit strategic solutions aimed at avoiding resource waste.MethodsThis paper aims to calibrate procedures to apply the method of moment quantile regression (MMQR) model to address this issue. Firstly, cross-sectional dependence (CSD) among the variables is examined. Next, a stable long-term relationship between the variables is assessed using stationarity analysis. Finally, the MMQR estimation is conducted to thoroughly investigate the impact of independent variables on CWQ and CO2 across different quantiles.ResultsThe results from both the fixed effects (FE-OLS) and dynamic ordinary least squares (D-OLS) models reveal stable and significant correlations between the regressors and response variables. The research findings indicate that GFP and FT exert a significant impact on improving both CWQ and reducing CO2. In contrast, the favorable growth of the TR sector contributes negatively to CWQ and CO2.DiscussionThe paper recommends that the government increase spending and investment in green projects utilizing renewable energy, green transportation, blockchain technology, and advanced techniques. It also advocates for a strategic approach to controlling TR, focusing on enhanced waste management, in order to improve CWQ and CO2 indicators across most quantiles.
Serum proteomic and metabolomic profiling of hepatocellular carcinoma patients co-infected with Clonorchis sinensis
Authors: Tang Zeli , Wei Caibiao , Deng Xueling , Lin Qiumei , Hu Qiping , Li Shitao , Wang Jilong , Wu Yuhong , Liu Dengyu , Fang Min , Zhan Tingzheng 
Published: January 2025

BackgroundClonorchis sinensis (C. sinensis) infection is a significant risk factor for hepatocellular carcinoma (HCC), yet its underlying mechanisms remain poorly understood. This study aimed to investigate the impact of C. sinensis infection on the serum proteomic and metabolomic profiling of HCC patients, focusing on the potential mechanisms.MethodA retrospective clinical analysis was conducted on 1121 HCC patients, comparing those with and without C. sinensis infection. The influence of C. sinensis on serum proteome and metabolome in HCC was further assessed.ResultC. sinensis infection correlated with a younger age at cancer onset, male predominance, advanced cancer stage, liver cirrhosis, and microvascular invasion in HCC patients. It also associated with shorter overall survival (OS) and recurrence-free survival (RFS). The levels of blood lipids (e.g., APO-A, HDL-C, and TG) were significantly altered after C. sinensis infection. Proteomic and metabolomic analyses revealed metabolic reprogramming caused by C. sinensis, with excessive depletion of argininosuccinate synthase (ASS) and D-glucose as potential factors in C. sinensis-associated HCC malignancy. Key molecules ILF2, CNN2, OLFM4, NOTCH3, and LysoPA were implicated in HCC progression. Furthermore, C. sinensis triggered inflammation, insulin resistance, and pro-tumor immune escape, and exacerbated the complication of degenerative diseases.ConclusionThis study not only provides compelling evidence for elucidating the mechanisms underlying C. sinensis-mediated HCC development but also identifies potential therapeutic targets for HCC patients co-infected with C. sinensis.
Murine model of cross-IgE sensitization and cross-anaphylactic reactions among multiple group food allergens
Authors: Musa Ibrahim , Ardalani Fariba , Yang Nan , Maleki Soheila J. , Li Xiu-Min 
Published: January 2025

RationaleApproximately 32 million people in the United States suffer from food allergies. Some food groups, such as legumes – peanuts, tree nuts, fish, and shellfish, have a high risk of cross-reactivity. However, the murine model of multiple food group cross-reactivity is limited.ObjectiveWe sought to develop a murine model that can be used to investigate novel therapeutics for the treatment of multiple food allergies.MethodsC3H/HeJ mice were sensitized intraperitoneally (i.p.) once a week for three weeks with a mixture of 500µg of protein from peanut, cashew, walnut, shrimp, cod, and 2 mg Alum. The control group consisted of naïve mice. IgE levels against the sensitized allergens and their cross-reactive allergens were measured by ELISA at baseline and 3 weeks after sensitization. In weeks 4 and 5, the mice were given intragastric challenges with 200mg/mouse of each food: peanut, chickpea, lentil, cashew, almond, pistachio, hazelnut, brazil nut, walnut, pecan, shrimp, lobster, cod, salmon, and mackerel. After each challenge, anaphylactic symptoms, rectal temperatures, and plasma histamine were measured.ResultsThere was a significant elevation of IgE against sensitized antigens (peanut cashew, walnut, shrimp, and cod) as well as cross-reactive allergens used for oral food challenge from legumes including peanut, chickpea, and lentil, as well as tree nuts such as cashew, almond, pistachio, hazelnut, brazil nut, walnut, and pecan. Furthermore, there was a significant increase in crustaceans such as shrimp, lobster and fish like cod, salmon, and mackerel (p&lt;0.01). Consistently, significantly increased anaphylactic symptom scores (p&lt;0.05), decreased rectal temperature (p&lt;0.001), and increased plasma histamine (p&lt;0.05) compared to the naïve mice occurred following each challenge with sensitized foods and unsensitized, but cross-reactive foods.ConclusionWe generated a comprehensive murine model of IgE-mediated multiple food groups of cross-reactive anaphylaxes. This will provide an essential tool for developing novel therapies for cross-reactivity multiple food allergies.
Case report: Reversible splenial lesion syndrome preceding the onset of multiple sclerosis
Authors: Mauritz Matthias , Kliushnikova Dariia , Otto Ferdinand , Harrer Andrea , Moser Tobias , Radlberger Richard Friedrich , Kleindienst Waltraud , Trinka Eugen , Wipfler Peter 
Published: January 2025

BackgroundThe reversible splenial lesion syndrome is frequently associated with systemic and central nervous system infections. Whether an infection associated with the occurrence of the reversible splenial lesion syndrome could play a role in the later development of multiple sclerosis is unknown.MethodsCase Report.ResultsA 27-year-old woman developed an infection-related reversible splenial lesion syndrome. Diagnostic findings did not establish a specific type of infection, but revealed evidence for a potential disposition towards autoimmunity. 32 months after the initial presentation, new clinical and radiological manifestations developed that led to a diagnosis of multiple sclerosis.ConclusionsIn susceptible individuals, infectious disease processes involving the central nervous system, such as described in this case, might be a factor in the pathogenesis of multiple sclerosis. More research on the prodromal stage of multiple sclerosis is needed to better understand the relationship between infections and autoimmunity.
Efficacy of traditional Chinese medicine Cordyceps sinensis as an adjunctive treatment in patients with renal dysfunction: a systematic-review and meta-analysis
Authors: Wu Fenfang , Xu Chunhua , Si Xinlei , He Fei , Xu Kang , Zhang Yu , Lin Shan 
Published: January 2025

ObjectiveThe effectiveness of using Cordyceps sinensis as an adjuvant therapy for patients with renal dysfunction (RD), especially acute kidney injury (AKI), is still a topic of debate. In response to the current conflicting data, the present meta-analysis was conducted to assess the clinical effectiveness of C. sinensis in the treatment of RD and to provide evidence for clinical practice.MethodsSeveral databases, including PubMed, EMBASE, Cochrane Library, China National Knowledge Infrastructure (CNKI) and Wanfang, were systematically searched updated to March 25, 2024. We used the combined ratio (OR) and diagnostic ratio (DOR) to assess the therapeutic effect of C. sinensis. In addition, risk of bias was assessed by Cochrane Risk of Bias Assessment Tool.ResultsThe present meta-analysis ultimately incorporated 15 studies comprising a total of 1,310 patients with RD. We pooled estimated the sensitivity, specificity as well as DOR from patient-based analyses with 0.89 (95% confidence interval [CI]: 0.84–0.93), 0.69 (95% CI: 0.59–0.77) and 18.0 (95% CI: 8.0–39.0), respectively. Moreover, we calculated the combined positive likelihood ratio (PLR) as well as negative likelihood ratio (NLR) to be 2.8 (95% CI: 2.1–3.9) and 0.16 (95% CI: 0.10–0.27), respectively. Additionally, area under the curve (AUC) of the summary receiver operating characteristic (SROC) was calculated as 0.88 (95% CI: 0.85–0.90) reflecting prognostic accuracy. Subsequently, subgroup analysis indicated that the clinical efficacy of C. sinensis in northern Chinese patients with RD was superior to that of southern. On the other hand, C. sinensis significantly reduced patients’ blood creatinine levels, shortened the oliguria period, and increased urine osmolality, indicating it can improve the function of glomeruli and renal tubules.ConclusionOur results indicate that C. sinensis can be considered a dependable clinical treatment for individuals with RD. It may improve the function of glomeruli and tubules, promote the recovery of tubular function, and thus enhance the clinical therapeutic effects.Systematic review registrationwww.crd.york.ac.uk/PROSPERO/#recordDetails, identifier CRD42024559042.
Dynamic assessment of long-term survival in survivors with stage III non-small cell lung cancer: a novel conditional survival model with a web-based calculator
Authors: Meng Xiangdi , Wang Peihe , Liu Jie , Sun Daqing , Ju Zhuojun , Cai Yuanyuan 
Published: January 2025

BackgroundConditional survival (CS) analysis can estimate further survival probabilities based on the time already survived, providing dynamic updates for prognostic information. This study aimed to develop a CS-nomogram to promote individualized disease management for stage III non-small cell lung cancer (NSCLC).MethodsThis study included patients diagnosed with stage III NSCLC in the Surveillance, Epidemiology, and End Results database from 2010 to 2017 (N = 3,512). The CS was calculated as CS(y|x) = OS(y + x)/OS(x), where OS(y + x) and OS(x) were the overall survival (OS) in the year (y + x) and year x, respectively, calculated by the Kaplan–Meier method. We used the least absolute shrinkage and selection operator (LASSO) regression to identify predictors and developed the CS-nomogram based on these predictors and the CS formula.ResultsThe CS analysis provided real-time updates on survival, with 5-year OS improving dynamically from 14.4 to 29.9%, 47.9, 66.0, and 80.8% (after 1–4 years of survival). Six independent predictors (age, tumor size, N status, surgery, radiotherapy and chemotherapy) were identified for the development of the CS-nomogram and its web version (https://dynapp.shinyapps.io/NSCLC/). The model performed with an excellent concordance index (C-index) of 0.71 (95% CI: 0.70–0.72), and a median time-dependent AUC of 0.71–0.73 from 200 iterations 5-fold cross-validation.ConclusionThe study demonstrated the improvement in real-time OS over time in stage III NSCLC survivors and developed the novel CS-nomogram to provide patients with updated survival data. It provided novel insights into clinical decisions in follow-up and treatment for survivors, offering a convenient tool for optimize resource allocation.
Research on a traditional Chinese medicine case-based question-answering system integrating large language models and knowledge graphs
Authors: Duan Yuchen , Zhou Qingqing , Li Yu , Qin Chi , Wang Ziyang , Kan Hongxing , Hu Jili 
Published: January 2025

IntroductionTraditional Chinese Medicine (TCM) case records encapsulate vast clinical experiences and theoretical insights, holding significant research and practical value. However, traditional case studies face challenges such as large data volumes, complex information, and difficulties in efficient retrieval and analysis. This study aimed to address these issues by leveraging modern data techniques to improve access and analysis of TCM case records.MethodsA total of 679 case records from Wang Zhongqi, a renowned physician of Xin’an Medicine, a branch of TCM, covering 41 diseases, were selected. The study involved four stages: pattern layer construction, knowledge extraction, integration, and data storage and visualization. A large language model (LLM) was employed to automatically extract key entities, including symptoms, pathogenesis, treatment principles, and prescriptions. These were structured into a TCM case knowledge graph.ResultsThe LLM successfully identified and extracted relevant entities, which were then organized into relational triples. A TCM case query system based on natural language input was developed. The system’s performance, evaluated using the RAGAS framework, achieved high scores: 0.9375 in faithfulness, 0.9686 in answer relevancy, and 0.9500 in context recall; In human evaluations, the levels of safety and usability are significantly higher than those of LLMs without using RAG.DiscussionThe results demonstrate that integrating LLMs with a knowledge graph significantly enhances the efficiency and accuracy of retrieving TCM case information. This approach could play a crucial role in modernizing TCM research and improving access to clinical insights. Future research may explore expanding the dataset and refining the query system for broader applications.
Effectiveness of the application of a digitalized repository on the quality of graduate and post-graduate education in the field of physical medicine and rehabilitation
Authors: Koleva Ivet B. , Yoshinov Borislav R. , Yoshinov Radoslav R. 
Published: January 2025

BackgroundDuring last ten years, we have developed a digital library with educational materials in Physical medicine and rehabilitation.ObjectivesThe objective of current article is the preparation of an electronic library with educational materials in the area of physical medicine, physical therapy and rehabilitation, and the comparative evaluation of the impact of this repository on the quality of education of students and trainees in the field.MethodologyThe electronic library includes e-books on different topics, elements of the specialty “Physical and rehabilitation medicine (PRM)” or Physiatry – with theoretical data, practical issues and case reports with videos of real patients. From the field of basic physiatry we included: books on kinesiological and pathokinesiological analysis, functional evaluation; physiotherapy (active exercises, soft-tissue techniques), ergotherapy (activities), balneotherapy (mineral baths and peloids, underwater gymnastics), manual therapy (tractions, mobilizations, manipulations), preformed physical factors (electric currents, magnetic field, ultrasound, light, laser, etc). In special physiatry we prepared manuals on: neurological and neurosurgical rehabilitation, orthopedic and traumatologic rehabilitation, rheumatologic rehabilitation, cardiac and cardiosurgical rehabilitation, etc. The digital library has been applied to educate 1,186 learners (graduate and post-graduate studies): students in Bachelor’s degree (Physiotherapy, Nursing, Midwifery, Medical assistants) and in Master’s degree (Physiotherapy, Medical rehabilitation and balneology), medical doctors—residents in Physical and Rehabilitation medicine (PRM), participants in long-life learning courses (physiatrists, physiotherapists and nurses). At the end of every educational course, we collected data from the practical and theoretical exams (investigation period—from April 2020 to October 2024). Additionally, we evaluated learners’ opinion using anonymous questionnaires on the usefulness of the electronic library.ResultsComparative analysis of the results revealed significant improvement in theoretical knowledge and practical skills of students and trainees, as evidenced by online assessments, and face-to-face theoretical and practical exams. The majority of learners consider the electronic repository useful.Conclusion and recommendationsThe authors consider this electronic library (with books, manuals, video-presentations and case studies) a valuable resource in both graduate and post-graduate education within the rehabilitation field. We recommend the application of electronic repositories in the process of education of the students and trainees.
Comparing the corneal temperature of dry eyes with that of normal eyes via high-resolution infrared thermography
Authors: Wu Chunbo , Huang Yuanshen , Xu Banglian , Li Baicheng , Zhuang Songlin , Cao Guofan , Hu Yan , Gu Zhensheng 
Published: January 2025

PurposeThis study compares the corneal temperature in dry eyes with normal eyes via high-resolution infrared thermography.MethodsA total of 86 participants were enrolled, with 40 and 46 participants in the dry eye disease (DED) and control groups, respectively. All participants underwent non-invasive breakup time (NIBUT) measurement, an Ocular Surface Disease Index (OSDI) questionnaire and ocular thermography.ResultsIn the DED group, the mean initial central corneal temperature (initial CCT) is 33.25 ± 0.66°C, the tenth-second central corneal temperature (10s-CCT) is 32.47 ± 0.84°C, and the mean change in central corneal temperature measured within 10 s (change in CCT within 10 s) is 0.78 ± 0.30°C. For the controls, the initial CCT, 10s-CCT, and change in CCT within 10 s are 33.14 ± 1.02°C, 32.90 ± 0.99°C, and 0.23 ± 0.20°C, respectively. Except for the initial CCT (p = 0.549), significant differences are observed in the 10s-CCT (p = 0.034) and the change in CCT within 10 s (p &lt; 0.001) between the two groups. The standard deviation of the temperature values within the region of interest (SD of TVs within ROI) on the central cornea is calculated to compare the uniformity of corneal temperature. In the DED group, the mean standard deviation of the initial temperature values within the region of interest (SD of initial TVs within ROI) is similar to that in the control group (0.23 ± 0.07°C vs. 0.22 ± 0.05°C, p = 0.926). In contrast, the mean standard deviation of the tenth-second temperature values within the region of interest (SD of 10s-TVs within ROI) in the DED group is greater than that in the control group, and there is a significant difference (0.44 ± 0.20°C vs. 0.35 ± 0.15°C, p = 0.016). In the DED group, the mean change in CCT within 3 s after tear film break-up is significantly greater than that before tear film break-up (0.19 ± 0.08°C vs. 0.10 ± 0.10°C, p &lt; 0.001).ConclusionAs the time with eyes open increases, dry eyes present a significantly faster decrease in central corneal temperature (CCT) and a significantly worse uniformity of corneal temperature compared with normal eyes.