bluuebunny/crossref_metadata_2025_split huggingface dataset:
Loss of TRPC2 function in mice alters sex differences in brain regions regulating social behaviors
Authors: Pfau Daniel R. , Baribeau Sarah , Brown Felix , Khetarpal Niki , Marc Breedlove S. , Jordan Cynthia L. 
Published: July 2023

AbstractThe transient receptor potential cation channel 2 (TRPC2) conveys pheromonal information from the vomeronasal organ (VNO) to the brain. Both male and female mice lacking this gene show altered sex‐typical behavior as adults. We asked whether TRPC2, highly expressed in the VNO, normally participates in the development of VNO‐recipient brain regions controlling mounting and aggression, two behaviors affected by TRPC2 loss. We now report significant effects of TRPC2 loss in both the posterodorsal aspect of the medial amygdala (MePD) and ventromedial nucleus of the hypothalamus (VMH) of male and female mice. In the MePD, a sex difference in neuron number was eliminated by the TRPC2 knockout (KO), but the effect was complex, with fewer neurons in the right MePD of females, and fewer neurons in the left MePD of males. In contrast, MePD astrocytes were unaffected by the KO. In the ventrolateral (vl) aspect of the VMH, KO females were like wildtype (WT) females, but TRPC2 loss had a dramatic effect in males, with fewer neurons than WT males and a smaller VMHvl overall. We also discovered a glial sex difference in VMHvl of WTs, with females having more astrocytes than males. Interestingly, TRPC2 loss increased astrocyte number in males in this region. We conclude that TRPC2 normally participates in the sexual differentiation of the mouse MePD and VMHvl. These changes in two key VNO‐recipient regions may underlie the effects of the TRPC2 KO on behavior.
Secure identity key and blockchain‐based authentication approach for secure data communication in multi‐WSN
Authors: Ganeshan Arulkumaran , Jayagopalan Santhosh , Perumal Balamurugan , Sarveshwaran Velliangiri 
Published: July 2023

SummaryBlockchain technology is an effective way to protect data that is handled by wireless sensor network (WSN). The traditional authentication protocol relies on trusted third parties, and most of the WSN paradigm does not allow these conditions, which results in single‐point failure. Sometimes the authentication process is slower, eventually hampers user experience, and thus users are inclined towards other alternatives that can offer multi‐factor authentication. To solve such issues, a secure identity key and blockchain‐based authentication framework is designed using a hybrid blockchain system in multi‐WSN to enhance the system's security. The nodes cooperate in the network to perform specific tasks such that interaction between nodes ensures the legitimacy of node's identity. With hybrid blockchain technology, nodes' identity information is authenticated and stored in the network after verification to establish communication among ordinary nodes and between the ordinary nodes and end user. The proposed method obtains higher security by achieving less computation time, higher detection rate, and limited memory usage of 0.072 s, 92.48%, and 3.925 MB, respectively. The developed authentication method is proved capable of proving resistance in case of different attacks in the network.
Hsa_circ_0057105 modulates a balance of epithelial‐mesenchymal transition and ferroptosis vulnerability in renal cell carcinoma
Authors: Cen Junjie , Liang Yanping , Feng Zihao , Chen Xu , Chen Jinlong , Wang Yinghan , Zhu Jiangquan , Xu Quanhui , Shu Guannan , Zheng Wenbin , Liang Hui , Wang Zhu , Deng Qiong , Cao Jiazheng , Luo Junhang , Jin Xiaohan , Huang Yong 
Published: July 2023

AbstractBackgroundThe incidence of renal cell carcinoma (RCC) has increased in recent years. Metastatic RCC is common and remains a major cause of mortality. A regulatory role for circular RNAs (circRNAs) in the occurrence and progression of RCC has been identified, but their function, molecular mechanisms, and potential clinical applications remain poorly understood.MethodsHigh‐throughput RNA sequencing was used to explore the differential expression of circRNAs and their related pathways in RCC patients. Transwell and CCK‐8 assays were used to assess the function of hsa_circ_0057105 in RCC cells. The clinical relevance of hsa_circ_0057105 was evaluated in a cohort of RCC patients. The hsa_circ_0057105 regulatory axis was defined using RNA pull‐down, luciferase reporter assays, and fluorescence in situ hybridization assays, and the in vivo effect of hsa_circ_0057105 was validated using animal experiments.ResultsSingle‐sample gene set enrichment analysis and correlation analysis of RNA‐seq data showed that hsa_circ_0057105 was potentially oncogenic and may serve to regulate epithelial‐mesenchymal transition (EMT) activation in RCC. Hsa_circ_0057105 expression was associated with advanced TNM stages and was an independent prognostic factor for poor RCC patient survival. Phenotypic studies show that hsa_circ_0057105 can enhance the migration and invasion abilities of RCC cells. Further, hsa_circ_0057105 was shown to inhibit the expression of miR‐577, a miRNA that regulated the expression of both COL1A1, which induced EMT activation, and VDAC2, which modulated ferroptosis sensitivity. The dual regulatory roles of hsa_circ_0057105 on EMT and ferroptosis sensitivity were verified using rescue experiments. Animal studies confirmed that hsa_circ_0057105 increased the metastatic ability and ferroptosis sensitivity of RCC cells in vivo.ConclusionsIn RCC, hsa_circ_0057105 regulates COL1A1 and VDAC2 expression through its sponge effect on miR‐577, acting like a ‘double‐edged sword’. These findings provide new insight into the relationship between EMT and ferroptosis in RCC and provide potential biomarkers for RCC surveillance and treatment.
Anibal Quijano: (Dis)entangling the geopolitics and coloniality of curriculum
Authors: Fúnez‐Flores Jairo I. 
Published: July 2023

AbstractThis essay reviews and builds upon Aníbal Quijano's contribution to decolonial theory to sketch out what I refer to as the geopolitics and coloniality of curriculum, broadly understood as an imperial doctrine and a pedagogical mode of domination aimed at producing a modern/colonial subjectivity. It argues that the geopolitics and coloniality of the curriculum reveal the relationship between geopolitical designs, colonialism, and curriculum, thereby contributing to the interrogation of how dominant ways of knowing are propagated discursively and pedagogically. The article focuses on how the geopolitics and coloniality of curriculum enable the reading, interpretation and unsettling of curricular discourses and pedagogical practices reproducing Euro‐Anglo‐American ways of being (ontological violence), individualist ways of knowing (epistemic violence) and racialised affective grammars. It concludes by gesturing towards ways to think, be, act, relate and do otherwise.
Neutrophil subsets in noncancer liver diseases: Cellular crosstalk and therapeutic targets
Authors: Huang Chen , Fan Xiaoli , Shen Yi , Shen Mengyi , Yang Li 
Published: July 2023

AbstractNeutrophils are the most abundant circulating granulocytes, linking innate and adaptive immunity. Neutrophils can regulate inflammatory and immune responses through degranulation, reactive oxygen species generation, the production of cytokines and chemokines, and NETosis. Emerging evidence has indicated that neutrophils contribute to the pathogenesis of various noncancer liver diseases, including nonalcoholic fatty liver disease, alcohol‐associated liver disease, hepatic ischemia‒reperfusion injury, and liver fibrosis. Cellular interactions among neutrophils, other immune cells, and nonimmune cells constitute a complex network that regulates the immune microenvironment of the liver. This review summarizes novel neutrophil subtypes, including CD177+ neutrophils and low‐density neutrophils. Moreover, we provide an overview of the cellular cros stalk of neutrophils in noncancer liver diseases, aiming to shed new light on mechanistic studies of novel neutrophil subtypes. In addition, we discuss the potential of neutrophils as therapeutic targets in noncancer liver diseases, including inhibitors targeting NETosis, granule proteins, and chemokines.
Research progress on pesticide residue detection based on microfluidic technology
Authors: Zhu Lv , Wu Mengyao , Li Ruiyu , Zhao Yunyan , Lu Yang , Wang Ting , Du Leilei , Wan Li 
Published: July 2023

AbstractThe problem of pesticide residue contamination has attracted widespread attention and poses a risk to human health. The current traditional pesticide residue detection methods have difficulty meeting rapid and diverse field screening requirements. Microfluidic technology integrates functions from sample preparation to detection, showing great potential for quick and accurate high‐throughput detection of pesticide residues. This paper reviews the latest research progress on microfluidic technology for pesticide residue detection. First, the commonly used microfluidic materials are summarized, including silicon, glass, paper, polydimethylsiloxane, and polymethyl methacrylate. We evaluated their advantages and disadvantages in pesticide residue detection applications. Second, the current pesticide residue detection technology based on microfluidics and its application to real samples are summarized. Finally, we discuss this technology's present challenges and future research directions. This study is expected to provide a reference for the future development of microfluidic technology for pesticide residue detection.
Characterisation of pressure‐concentration‐temperature profiles for metal hydride hydrogen storage alloys with model development
Authors: Ge Y. T. 
Published: July 2023

AbstractMetal hydride (MH) alloys have been applied to hydrogen storage and various energy conversion systems such as refrigeration, heat pump and heat transformer. However, to facilitate and efficiently investigate efficiently a particular application, an MH alloy must firstly be characterised with a purposely built test facility to measure profiles of pressure, MH hydrogen concentration and temperature (PCT). Obtaining detailed PCT profiles or curves could be an arduous and expensive task as each isothermal hydrogen absorption or desorption line requires hundreds of measurement points. It is thus desirable to develop an accurate correlative model for the PCT profiles with limited measurements of thermophysical property data for the purpose of characterisation of each MH alloy. This correlative model or characterisation process has been developed and is described in detail in this article. The correlative PCT MH alloy profiles can cover all applicable hydrogen storage phase regions of α, α + β and β as well as the phase transition dome curve and critical point such that a PCT phase diagram for a particular MH alloy can be depicted and characterised. As an application example, the correlative model is applied to predict an MH alloy's hydrogen storage capacity and hysteresis at a specific MH temperature. It has been discovered that each of these two parameters shows comparative trends in variation with reduced temperature. Correspondingly, for each parameter, a correlative function with reduced temperature has been produced. The MH alloy characterisation process is an essential step towards a detailed dynamic MH energy system modelling, simulation and optimisation as well as experimental investigation.
Optimization of port injection of n‐decanol in a PCCI engine using response surface methodology
Authors: Vinodkumar V. , Karthikeyan A. , Jayaprabakar J. , Senthilkumar G. , Ranganathan L. 
Published: July 2023

AbstractThis work aims to define the optimum n‐decanol fraction in the inlet port and the corresponding engine load for the better emissions and performance characteristics of a partially premixed charged compression ignition (PCCI) engine by response surface methodology (RSM). The numerical model based on multi‐linear regression was established using experimental data. For this, the influence of various proportions of n‐decanol through intake port including 10%, 20%, 30%, and 40% were experimentally investigated besides the primary injection of neem oil biodiesel in the volumetric ratio of 80% diesel and 20% neem biodiesel, namely NB20. The optimization using RSM is exploited to capitalize the brake thermal efficiency (BTE) and diminish the emissions including oxides of nitrogen (NOx), carbon monoxide (CO) emission, smoke opacity, and hydrocarbon (HC) emission. The n‐decanol fraction in the port injection of 31.43% and the engine load in terms of brake power of 2.950 kW were found to be optimum parameters with the maximum desirability of 0.752. The optimal responses for brake‐specific fuel consumption (BSFC), BTE, CO, HC, smoke, and NOx under these operating conditions were found to be 0.305 kg/kWh, 28.8%, 0.145%, 19.61%, 54.85 ppm, and 837.7 ppm, respectively. Likewise, the correlation coefficient R2 values for BSFC, BTE, CO, HC, smoke and NOx have been found to be 99.85%, 99.95%, 93.58%, 90.32%, 99.97%, and 99.93%, respectively. According to the study's findings, the RSM is a realistic method for calculating and enhancing a diesel engine's emission and performance values operating in PCCI mode and using n‐decanol and NB20 as fuels.
Searching for prognostic markers for Stage I epithelial ovarian cancer: A role for systemic inflammatory markers
Authors: Borella Fulvio , Bertero Luca , Valabrega Giorgio , Fucina Stefano , Cassoni Paola , Benedetto Chiara 
Published: July 2023

AbstractObjectiveTo determine the prognostic role of systemic inflammatory markers for Stage I epithelial ovarian cancer (EOC).Materials and MethodsWe performed a retrospective, single‐center, observational study. We included patients with Stage I EOC cancer undergoing primary surgery between 1993 and 2016. Inflammatory markers were assessed by analyzing blood samples collected at initial diagnosis before EOC surgery. We evaluated these markers' association with disease‐free survival (DFS) and cancer‐specific survival (CSS).ResultsWe included 176 women in our study. The neutrophil‐to‐lymphocyte ratio (NLR), platelet‐to‐lymphocyte ratio (PLR), and systemic immune inflammation index (SII) were related to both DFS and CSS in the univariate analysis. In the multivariate Cox analysis, adjuvant chemotherapy (hazard ratio [HR] 0.17, 95% confidence interval [CI] 0.04–0.71, P = 0.02) and SII ≥730 (HR 6.84, 95% CI 1.30–35.9, P = 0.023) were independent predictors of DFS, while FIGO Stage IB–IC (HR 7.91, 95% CI 1.04–59.8, P = 0.04), NLR ≥3 (HR 56.8, 95% CI 7.46–433, P < 0.001) and PLR ≥169 (HR 49.1 95% CI 11.1–217.8, P = 0.005) were independent predictors of CSS.ConclusionsSystemic inflammatory markers are easily obtainable from patients' routine blood analyses and may represent inexpensive and reproducible prognostic markers in early‐stage EOC.
Development of tools to facilitate the diagnosis of hereditary fructose intolerance
Authors: Panis Bianca , Janssen Lise E. F. , Lefeber Dirk J. , Simons Nynke , Rubio‐Gozalbo M. Estela , Brouwers Martijn C. G. J. 
Published: July 2023

AbstractAlthough hereditary fructose intolerance (HFI) is an inborn error of fructose metabolism that classically presents at infancy, the diagnosis is often missed or delayed. In this study, we aimed to develop tools to facilitate the diagnosis of HFI. The intake of fructose‐containing food products, that is, fruit, fruit juice and sugar‐sweetened beverages, was assessed by a 3‐day food diary in adult HFI patients (n = 15) and age, sex, and BMI‐matched controls (n = 15). Furthermore, glycosylation of transferrin was examined using high‐resolution mass spectrometry and abnormally glycosylated transferrin was expressed as ratio of normal glycosylated transferrin. We found that the sensitivity and specificity of the 3‐day food diary for the intake of at least one fructose‐containing food product were both 100%. Both mono‐glyco:diglyco transferrin and a‐glyco+mono‐glyco:di‐glyco transferrin were greater in HFI patients and had a high‐discriminatory power (area under the receiver operating characteristic curve: 0.97 and 0.94, respectively). In this well‐characterized cohort of adult HFI patients, the 3‐day food questionnaire and the glycosylation pattern of transferrin are valuable tools to facilitate the recognition and diagnosis of HFI in adult patients.
Pharmacomodulation of brain neuromedin U signaling as a potential therapeutic strategy
Authors: Pałasz Artur , Worthington John J. , Filipczyk Łukasz , Saganiak Karolina 
Published: July 2023

AbstractNeuromedin U (NMU) belongs to a family of multifunctional neuropeptides that modulate the activity of several neural networks of the brain. Acting via metabotropic receptor NMUR2, NMU plays a role in the regulation of multiple systems, including energy homeostasis, stress responses, circadian rhythms, and endocrine signaling. The involvement of NMU signaling in the central regulation of important neurophysiological processes and its disturbances is a potential target for pharmacological modulation. Number of preclinical studies have proven that both modified NMU analogues such as PASR8‐NMU or F4R8‐NMU and designed NMUR2 agonists, for example, CPN‐116, CPN‐124 exhibit a distinct pharmacological activity especially when delivered transnasally. Their application can potentially be useful in the more convenient and safe treatment of obesity, eating disorders, Alzheimer's disease‐related memory impairment, alcohol addiction, and sleep disturbances. Accumulating findings suggest that pharmacomodulation of the central NMU signaling may be a promising strategy in the treatment of several neuropsychiatric disorders.
Determining the twist angle of stacked MoS2layers using machine learning‐assisted low‐frequency interlayer Raman fingerprints
Authors: Tang Jing , Wang Zifan , Tan Qishuo , Cao Jun , Ling Xi 
Published: July 2023

AbstractThe investigation of twisted stacked few‐layer MoS2has revealed novel electronic, optical, and vibrational properties over an extended period. For the successful integration of twisted stacked few‐layer MoS2into a wide range of applications, it is crucial to employ a noninvasive, versatile technique for characterizing the layered architecture of these complex structures. In this work, we introduce a machine learning‐assisted low‐frequency Raman spectroscopy method to characterize the twist angle of few‐layer stacked MoS2samples. A feedforward neural network (FNN) is utilized to analyze the low‐frequency breathing mode as a function of the twist angle. Moreover, using finite difference method (FDM) and density functional theory (DFT) calculations, we show that the low‐frequency Raman spectra of MoS2are mainly influenced by the effect of the nearest and second nearest layers. A new improved linear chain model (TA‐LCM) with taking the twist angle into the consideration is developed to understand the interlayer breathing modes of stacked few‐layer MoS2. This approach can be extended to other 2D materials systems and provides an intelligent way to investigate naturally stacked and twisted interlayer interactions.
Utility of Extended High‐Frequency Audiograms in Clinical Practice
Authors: Saade Mia , Fernandez Karla , Little Christine , Schwam Zachary G , Cosetti Maura 
Published: July 2023

ObjectivesExtended high‐frequency (EHF) audiometry elicits pure‐tone thresholds at frequencies above 8 kHz, which are not included in routine clinical testing. This study explores the utility of EHF audiometry in patients with various audiologic symptoms despite normal‐hearing thresholds at ≤8 kHz.MethodsA retrospective review was performed of all patients receiving conventional (250–8 kHz) and EHF (9–20 kHz) audiometry at a tertiary otological referral center between April 2021 and August 2022. Only patients with audiologic symptoms and pure‐tone thresholds ≤25 dB HL at ≤8 kHz bilaterally on routine testing were included in subsequent analysis. EHF‐PTA was defined for each ear as an average of the air conduction thresholds at 9.0, 10.0, 11.2, 12.5, 14.0, 16.0, 18.0, and 20.0 kHz.ResultsOf the 50 patients who received EHF testing, 40 had audiologic symptoms and normal conventional audiograms at ≤8 kHz. Twenty‐five of the 40 (62.5%) were found to have hearing loss in the highest frequencies. Patients with EHF hearing loss (EHF‐HL) were more likely to report subjective hearing loss. Age was significantly greater in those with EHF‐HL compared with those without EHF‐HL, and age was positively correlated with the degree of EHF‐HL.ConclusionEHF testing correlates with audiologic symptoms in patients with normal testing at ≤8 kHz and may be considered when standard audiometry is normal. Additional data are warranted to create an evidenced‐based, clinical algorithm for EHF audiometry that can guide treatment, direct mitigation strategies, and potentially identify those at higher risk of hearing loss over time.Level of Evidence4 Laryngoscope, 134:907–910, 2024
Pediatric Inferior Turbinate Hypertrophy: Diagnosis and Management. A YO‐IFOS Consensus Statement
Authors: Maniaci Antonino , Calvo‐Henriquez Christian , Cammaroto Giovanni , Garcia‐Magan Carlos , Garcia‐Paz Vanesa , Iannella Giannicola , Jiménez‐Huerta Ignacio , La Mantia Ignazio , Lechien Jérome R. , Leong Samuel C. , Lobo‐Duro David , Maza‐Solano Juan , Mitchell Ron , Otero‐Alonso Andrea , Peng You , Radulesco Thomas , Simon François , Teissier Natasha , Cocuzza Salvatore , Saibene Alberto M. 
Published: July 2023

ObjectivePediatric inferior turbinate hypertrophy (PedTH) is a frequent and often overlooked cause or associated cause of nasal breathing difficulties. This clinical consensus statement (CCS) aims to provide a diagnosis and management framework covering the lack of specific guidelines for this condition and addressing the existing controversies.MethodsA clinical consensus statement (CCS) was developed by a panel of 20 contributors from 7 different European and North American countries using the modified Delphi method. The aim of the CCS was to offer a multidisciplinary reference framework for the management of PedTH on the basis of shared clinical experience and analysis of the strongest evidence currently available.ResultsA systematic literature review following the Preferred Reporting Items for Systematic Reviews and Meta‐Analyses (PRISMA) criteria was performed. From the initial 96 items identified, 7 articles were selected based on higher‐evidence items such as randomized‐controlled trials, guidelines, and systematic reviews. A 34‐statement survey was developed, and after three rounds of voting, 2 items reached strong consensus, 17 reached consensus or near consensus, and 15 had no consensus.ConclusionsUntil further prospective data are available, our CCS should provide a useful reference for PedTH management. PedTH should be considered a nasal obstructive disease not necessarily related to an adult condition but frequently associated with other nasal or craniofacial disorders. Diagnosis requires clinical examination and endoscopy, whereas rhinomanometry, nasal cytology, and questionnaires have little clinical role. Treatment choice should consider the specific indications and features of the available options, with a preference for less invasive procedures.Level of Evidence5 Laryngoscope, 134:1437–1444, 2024