bluuebunny/crossref_metadata_2025_split huggingface dataset:
Mastocytosis: retrospective analysis of maculopapular cutaneous form
Authors: Kasikhina E. I. , Potekaev N. N. , Kochetkov M. A. , Zhukova O. V. , Ostretsova M. N. , Mednikova M. A. , Bogdel A. M. 
Published: December 2022

Introduction. The article presents the data of a retrospective analysis of the debut and clinical manifestations of the types of maculopapular cutaneous mastocytosis (МPCM) in 96 children, and the frequency of non-regression of skin rashes with age in 33 adults. Aim. To conduct a retrospective analysis of the onset, clinical manifestations and dynamics of maculopapular cutaneous mastocytosis (MPCM). Materials and methods. Outpatient records of 96 children and 33 adults, who were treated and monitored at the Moscow Scientific and Practical Center of Dermatovenereology and Cosmetology, were statistically analyzed. Results. Monomorphic type of MPCM was diagnosed in 28 (29.2%) patients, polymorphic – in 68 (70.8%) patients. In 92.6% of children with a polymorphic type and in 35.7% with a monomorphic type, the rash debuted at the age of two years. The clinical picture of the monomorphic type was dominated by multiple small brown or yellow-brown spots. Rashes in the polymorphic type were spread, represented by brown or reddish-brown spots, papules, plaques ranging in size from several millimeters to several centimeters. The positive phenomenon of Unna-Darier was determined in 82.3% of cases. In patients with polymorphic type, the general population level of tryptase (<11.4 µg/l) was determined significantly more often than in patients with monomorphic type (p < 0.05). Elevated tryptase level was found in 53.6% of children and 69.7% of adults with monomorphic rashes. Pruritus was the most common cutaneous symptom, occurring in 49.0% of children. Bullous eruptions developed only in patients with polymorphic type. The most frequently reported systemic manifestations were gastrointestinal symptoms and headaches. Conclusions. The obtained results suggest that patients with early onset of the disease with polymorphic and variable lesions show a more favorable outcome.
Screening testing and computerized vestibulometry in vestibular neuronitis
Authors: Lilenko S. V. , Sugarova S. B. , Lilenko A. S. , Kostevich I. V. 
Published: December 2022

Introduction. The demonstrative example of unilateral peripheral vestibulopathy is vestibular neuronitis. This disease of infectious-toxic origin has the involvement of vestibular ganglia neurons or vestibular nerve fibers. The overwhelming moment in topic diagnostics of vestibular impairments is within-patient comparison anamnesis data and vestibulometry results. In cases of vestibular neuronitis exception of central vestibular system pathology is mandatory. Objective. To attain objectification of disequilibrium in vestibular neuronitis by means of registration and assessment of oculogyric and postural reactions. Materials and methods. Vestibular passport trials are recommended in the examination of patients with acute vertiginous complaints. Special attention has been given to the revelation of impairments of command eye movements, disturbances of ocular pursuit, spontaneous nystagmus, and catch-up saccades in Halmagyi – Curthoys test. Possible impairments of static and dynamic balance control are assessed in modified Romberg test and Unterberger test. Gaze tests with registration of saccadic and pursuit eye movements, spontaneous and optokinetic nystagmus reactions as well bithermal caloric test are carried out in computerized electrooculography and videooculography. Deviations of body gravity center are analyzed with the help of sensory organization test in computerized dynamic posturography. Results and discussion. The results of screening and computerized vestibular testing of 24 patients are presented. High diagnostic value of registration and analysis of oculogyric reactions as well as postural control signs is demonstrated in cases of acute vestibular neuronitis and acerbation of its chronic recurrent variant. The key objective symptom, which confirms peripheral level of vestibular dysfunction, is spontaneous nystagmus registration in elimination of gaze fixation. Unsteadiness in static and dynamic trials confirms the vestibular decompensation extent in acute phase of vestibular ganglia / neurons pathology in cases of vestibular neuronitis. Decompensation signs revelation of this peripheral vestibular dysfunction needs urgent medicine: possible etiotropic and obligate symptomatic therapy as well as spare vestibular rehabilitation exercises. Conclusion. Combination of oculogyric impairments and disturbances of postural control enables to improve accuracy of diagnostics in decompensated stage of vestibular neuronitis.
Exploration of the effect of simultaneous removal of EDCs in the treatment process of different types of wastewater
Authors: Wu Lieshan , Lu Chunliu , Huang Ning , Zhong Minjie , Teng Yunmei , Tian Yan , Ye Kaixiao , Liang Liuling , Hu Zaoshi 
Published: December 2022

Abstract
The SPE-HPLC-MS/MS method was used to investigate the concentration distribution of nine types of estrogens in 18 locations of pollution source along the Jiuzhou River belonging to river systems in Guangdong province and Guangxi Zhuang autonomous region in China, and the estrogenic activity and potential ecological risks were evaluated by calculating the estradiol equivalency (EEQ). The results showed that the calculated estradiol equivalents (cEEQs) of wastewater treatment plants from 17 locations were all higher than 1 ng/L. To further study the removal effect of the treatment process on the estrogens, the pig breeding wastewater from P4 and the municipal wastewater from P13, as well as the black-odorous water, were sampled and surveyed during the entire process. It turned out that estrogens were effectively removed after nitrification activated sludge treatment. Meanwhile, there was a positive correlation between the removal of NH3-N, total nitrogen (TN) and total phosphorus (TP) and the removal of endocrine disrupting chemicals (EDCs). It is shown in the study the secondary treatment process has achieved a significant effect on the removal of estrogen in both the wet and dry periods and that there has been a positive correlation between the activities of total phosphorus compounds, nitrogen-based compounds and the removal of EDCs.
The Status of Messianic Jews on mixed marriage in the canonicallegalorderanditscanonicalimplications
Authors: Wilk Jacek 
Published: December 2022

W zaprezentowanym opracowaniu przedmiotem uwagi Autora stała się kwestia statusu Żydów Mesjanistycznych w przedmiocie małżeństw mieszanych w kanonicznym porządku prawnym. Badając doktrynę wspólnoty Żydów Mesjanistycznych Autor wyka- zał, iż charakteryzuje się ona synkretyzmem, zawierając zarówno elementy judaistyczne, jak i chrześcijańskie, wśród których istotną staje się wiara w Jezusa Chrystusa jako Mesja- sza. Patrząc na tą doktrynę przez pryzmat regulacji dotyczących małżeństw mieszanych w prawie kanonicznym (kan. 1086, 1125 KPK/83) dowiódł, iż o statusie drugiej strony nie decyduje wiara w Chrystusa, ale przyjęty chrzest; tego sakramentu nie przyjmuje się na- tomiast u Żydów Mesjanistycznych. Stąd też traktować ich należy jako nieochrzczonych. W efekcie podpadają oni pod przeszkodę różności religii (kan. 1086 KPK/83).
Oprócz tego Autor wskazał, iż pokaźna część członków tej wspólnoty jest nie-Żydami, o proweniencji chrześcijańskiej, dlatego też należy ich traktować jako ochrzczonych; podpadają więc oni pod przeszkodę odmiennego wyznania (kan. 1125 KPK/83).
PAK1 Inhibition Suppresses the Proliferation, Migration and Invasion of
Glioma Cells
Authors: Shi Hengliang , Yi Qinghao , Chen Tianze , Zhou Kunlin , Ma Qiang , Sun Zhiyuan 
Published: December 2022

Background:
p21-activated kinase 1 (PAK1) is abnormally expressed in glioma, but its
roles and mechanisms in glioma remain unclear. This study aims to explore the effects of PAK1 inhibition
on the proliferation, migration and invasion of glioma cells.

Method:
Cell Counting Kit-8 (CCK-8), 5‐ethynyl‐20‐deoxyuridine (EdU) incorporation and colony
formation assays were performed to evaluate the effects of PAK1 inhibition on the proliferation of
glioma cells. The cell cycle distribution and apoptosis rate of glioma cells were explored by flow cytometry.
Wound healing and Transwell assays were performed to investigate the effects of PAK1 inhibition
on glioma cell migration and invasion. The orthotopic xenograft glioma model was used to
probe the effect of PAK1 silencing on glioma tumor formation.

Results:
PAK1 inhibition arrested cells at the G1 phase and induced apoptosis of glioma cells. Moreover,
the knockdown of PAK1 decreased the protein expression levels of MDM2, p38, p-p38, cyclin
D1, CDK4, Bcl-2, MMP2, MMP9, and cofilin but increased the protein levels of p53, Bax, p21 and
cleaved caspase-3. A xenograft glioma model confirmed that the silencing of PAK1 repressed the
formation of tumors induced by U87 cell transplantation.

Conclusion:
This study showed that PAK1 inhibition impedes the proliferation, migration, and invasion
of glioma cells.
Clinical, Genetic, and Pathological Features of very Early Onset Frontotemporal
Lobe Degeneration: A Systematic Review
Authors: Chu Min , Wu Liyong , Liu Li , Nan Haitian , Jiang Deming , Wang Yihao , Rosa-Neto Pedro 
Published: December 2022

Background:
In most patients with frontotemporal lobe degeneration (FTLD), the degenerative
process begins between the ages 45 and 65 years; onset younger than 45 years is relatively rare
and considered very early onset FTLD (VEO-FTLD).

Objective:
To delineate the clinical, genetic, and pathological features of VEO-FTLD.

Methods:
A systematic literature review was carried out in PubMed and Embase from inception to
September 2021. Patients diagnosed with definite FTLD with onset before age 45 years were included.
Patients lacking detailed clinical data or both genetic and neuropathological data were excluded.
Phenotypic, genotypic, and pathological data were extracted for further analyses.

Results:
Data from 110 patients with VEO-FTLD, reported in a cumulative 70 publications, were included.
Age of onset was 35.09 ± 7.04 (14-44) years. Sixty-seven patients were reported age at death
of 42.12 ± 7.26 (24–58) years, with a disease course lasting 8.13 ± 4.69 (1–20) years. Behavioural
variant frontotemporal dementia (104/110, 94.5%) was the most common clinical subtype, often manifesting
as disinhibition (81.8%) and apathy (80.9%), and frequently accompanied by a cognitive deficit
(90.9%) and parkinsonism (37.3%). Frequency of familial aggregation was high (familial vs. sporadic,
73/37, 66.4%); most patients carried MAPT gene mutations (72.9% in familial, 40% in sporadic),
followed by C9 (18.8% in familial, 10% in sporadic), TARDBP (2.1% in familial), and VCP
(2.1% in familial). The most common neuropathology subtype was tau (43.5%), followed by ubiquitin-
positive (24.6%), FUS (20.3%), and TDP 43 (2.9%).

Conclusions:
VEO-FTLD may have unique clinical, genetic, and neuropathological markers and
should be considered in young patients with psycho-behavioral symptoms.
Ethosomes: Novel Vesicular Carriers for Effective Transdermal Delivery of Natural Therapeutics
Authors: Bhatia Shiveena , Singh Varinder , Singh Manjinder , Singh Deepinder , Singh Tanveer , Piplani Mona , Singh Ravinder 
Published: December 2022

Abstract:
The topical delivery, being the most reliable route for drug administration, offers multiple advantages.
The conventional topical dosage forms deliver a relatively higher amount of drug to achieve
therapeutic action triggering hypersensitivity reactions accompanied by greasiness and staining issues.
The advent of transdermal nanocarriers has waived off some of these limitations and assisted in achieving
enhanced therapeutic efficacy with sustained release and minimal/no instances of systemic toxicity. The
ethosome, one of the novel lipid carrier systems, has eased the administration of many hydrophilic and
lipophilic drugs through the stratum corneum. It is a non-invasive drug carrier consisting of 45% ethanol,
phospholipids, and non-ionic surfactants. The discovery of ethosomal technology has proved to be fruitful
in delivering drugs with a wide range of polarity and other physicochemical parameters across skin. In
this review, the ethosomal system has been explored for the delivery of complex phytoconstituents across
the skin along with the key building material of ethosomes, associated mechanism of drug delivery, recent
developments in ethosomes as a drug carrier, reported ethosomal formulations encapsulating various plant
metabolites, conducted clinical trials and available ethosomal formulations for the delivery of phytocomponents
across stratum corneum.
Phytochemistry and Pharmacological Profile of Drumstick Tree “Moringa
oleifera Lam”: An Overview
Authors: Singh Sonia , Dubey Sudhanshu , Rana Nishu 
Published: December 2022

Background:
The herbal plant “Moringa oleifera Lam” is widely cultivated throughout
India. The plant belongs to the Moringaceae family. It is commonly called as Drumstick tree
and Horse-radish tree. Sometimes, the plant Moringa oleifera is called a “Miracle tree or Tree of
life.”

Objective:
The current manuscript summarizes the phytochemical constituents and pharmacological
properties of M.oleifera.

Methods:
Several pieces of literature have been searched in books, journals, and databases, including
ScienceDirect, Web of Science, Publons, and Scopus.

Results:
The fruits and leaves of M. oleifera are generally used as a vegetable in the Indian subcontinent
region. The whole parts of a plant contain valuable and essential therapeutic features; it
has the richest content of antiradical components and nutrients. Various secondary components
include hexacosone, pentacosane, phytol, carotenes, tocopherols, isothiocyanates, myricetin,
quercetin, kaempferol, rutin, chlorogenic acid, ferulic acid, sinalbin, and gallic acid. It contains a
higher percentage of vitamin A-E and vitamin K. Some mineral components include copper, calcium,
potassium, iron, magnesium, zinc, and manganese. It has approximately more than forty
naturally occurring antiradical constituents. The plant has diversified medicinal applications such
as antidiabetic, antiasthmatic, anti-inflammatory, anticancer, antifertility, antiallergic, antipyretic,
wound healing, analgesic, central nervous system activity, and hepatoprotective activities. The
plant Moringa oleifera Lam has been found effective in various infections and diseases.

Conclusion:
This article provides a detailed explanation of the phytochemical and pharmacological
activity of the plant that is being discussed.
Recent Advances on Natural and Non-Natural Xanthones as Potential
Anticancer Agents: A Review
Authors: Gogoi Urvashee , Pathak Kalyani , Saikia Riya , Pathak Manash Pratim , Paul Tirna , Khan Shah Alam , Das Aparoop 
Published: December 2022

Background:
Xanthones, natural or synthetic, due to their wide range of biological activities,
have become an interesting subject of investigation for many researchers. Xanthonic scaffold
has proven to have a vital role in anticancer drug development since many of its derivatives have
shown anticancer activities on various cell lines. In addition, targeting epigenetic markers in cancer
has yielded promising results. There have also been reports on the impact of xanthone and related
polyphenolic compounds on epigenetics markers in cancer prevention and therapy.

Objective:
The objective of this review is to comprehensively highlight the main natural and nonnatural
sources of xanthones having potential anti-cancer effects along with their key structural elements,
structure-activity relationships (SARs), mechanisms of action, and epigenetic profile of xanthone-
based anti-cancer compounds. The challenges and future directions of xanthone-based therapies
are also discussed briefly.

Method:
The methods involved in the preparation of the present review included the collection of all
recent information up to November 2021 from various scientific databases, indexed periodicals, and
search engines such as Medline Scopus, Google Scholar, PubMed, PubMed Central, Web of Science,
and Science Direct.

Results:
Exploration of the diversity of the xanthone scaffold led to the identification of several derivatives
having prominent anti-cancer activity. Their unique structural diversity and synthetic modifications
showed the ongoing endeavour of enriching the chemical diversity of the xanthone molecular
framework to discover pharmacologically interesting compounds. However, studies regarding
their modes of action, pharmacokinetic properties, clinical data, epigenetics, and safety are limited.

Conclusion:
Elucidation of the exact biological mechanisms and the associated targets of xanthones
will yield better opportunities for these compounds to be developed as potential anticancer drugs.
Further clinical studies with conclusive results are required to implement xanthones as treatment
modalities in cancer.
Anticancer Activity of 3,5-Bis(dodecyloxy)Benzoate-PAMAM Conjugates
with Indomethacin or Mefenamic Acid
Authors: Castillo-Rodríguez Irving Osiel , Pedro-Hernandez Luis Daniel , Ramírez-Ápan Teresa , Martínez-García Marcos 
Published: December 2022

aims:
The aim of the present investigation was the synthesis of 3,5-bis(dodecyloxy)benzoate -PAMAM conjugates with indomethacin an mefenamic acid to examine their anticancer activity.

background:
The synthesis of conjugates with nonsteroidal anti-inflammatory drugs could be improve their activity with less toxicity and these compounds could be used for the treatment of cancer.

objective:
The synthesis of 3,5-bis(dodecyloxy)benzoate -PAMAM conjugates with indomethacin or mefenamic acid to examine their anticancer activity.

method:
The anticancer activity was studied of the conjugates against six human cancer cells U-251 (human glioblastoma), PC-3 (human prostatic adenocarcinoma), K-562 (human chronic myelogenous leukemia cells), HCT-15 (human colorectal adenocarcinoma), MCF-7 (human mammary adenocarcinoma), SKLU-1 (human lung adenocarcinoma) and the COS-7 African green monkey (as a control) cell lines. The conjugates with indomethacin and mefenamic acid were characterized by 1H, 13C NMR one- and two-dimension spectroscopy.

result:
All the conjugates synthetized with indomethacin or mefenamic acid showed anticancer activity against all the human cancer cell lines. The first generation of indomethacin conjugates showed better activity against PC-3 cell line than the second generation. But the second generation with indomethacin showed better activity against PC-3 (human prostatic adenocarcinoma) than the first generation. The second generation conjugate with mefenamic acid had strong selectivity to PC-3 (human prostatic adenocarcinoma) cells with IC50 value of 10.23±1.2 M in vitro.

conclusion:
In the paper, we report the synthesis and spectroscopic analyses of new indomethacin or mefenamic acid conjugates. The overall results showed that the conjugate of second generation with mefenamic acid could be a potential nanocarrier for human prostatic adenocarcinoma cancer treatment.

other:
our research will be continued
Overview of High-Performance Thin Layer Chromatography and its
Applications in Pesticide Analysis
Authors: Kandasamy Ruckmani , Muruganantham Selvakumar , Krishnaswami Venkateshwaran , Alagarsamy Shanmugarathinam 
Published: December 2022

Abstract:
In recent decades, increased demand for food has been caused by a rapid rise in the human
population, which triggers agricultural intensification. To resist undesired pests from infecting crops,
farmers widely utilize pesticides to improve agricultural production during the pre-harvest period. Despite
the fact that pesticides cause a number of health risks, there is insufficient monitoring of these toxins.
Therefore, it is important to develop a specific, accurate, and efficient method for determining the pesticides
in varied samples in order to safeguard health against potential risks. Due to the lower concentrations
of active compounds and their diversity of availability, it is challenging to detect pesticide residues
in different samples. In this case, to effectively separate, identify, and accurately quantify pesticides at
low concentrations in a variety of samples, a reliable analytical methodology is needed. Recently, the
application of high-performance thin layer chromatography (HPTLC) offers a wider scope with excellent
separation, identification, and quantitative/qualitative determination in pesticide analysis. In spite of their
extremely low quantities, pesticide residues can be accurately and precisely identified using HPTLC.
HPTLC has a number of benefits, such as easy sample preparation, automation, densitometry, and hyphenation,
and is particularly well suited for identification and detection. Concerning this, the proposed
review paper provides an overview of stationary phases, mobile phases, sample applicators, visualization,
derivatization, and detection of HPTLC utilized for the identification and detection of pesticide residues
in agriculture and environmental samples.
Imbalance of Th1 and Th2 Cytokines and Stem Cell Therapy in
Pathological Pain
Authors: Yu Yao-Qing , Wang Huan 
Published: December 2022

Abstract:
The pathophysiological importance of T helper 1 (Th1) and Th2 cell cytokines in pathological
pain has been highly debated in recent decades. However, the analgesic strategy targeting individual
cytokines still has a long way to go for clinical application. In this review, we focus on the contributions
of Th1 cytokines (TNF-α, IFN-γ, and IL-2) and Th2 cytokines (IL-4, IL-5, IL-10, and IL-13)
in rodent pain models and human pain-related diseases. A large number of studies have shown that
Th1 and Th2 cytokines have opposing effects on pain modulation. The imbalance of Th1 and Th2 cytokines
might determine the final effect of pain generation or inhibition. However, increasing evidence
indicates that targeting the individual cytokine is not sufficient for the treatment of pathological pain.
It is practical to suggest a promising therapeutic strategy against the combined effects of Th1 and Th2
cytokines. We summarize the current advances in stem cell therapy for pain-related diseases. Preclinical
and clinical studies show that stem cells inhibit proinflammatory cytokines and release enormous
Th2 cytokines that exhibit a strong analgesic effect. Therefore, a shift of the imbalance of Th1 and Th2
cytokines induced by stem cells will provide a novel therapeutic strategy against intractable pain. It is
extremely important to reveal the cellular and molecular mechanisms of stem cell-mediated analgesia.
The efficiency and safety of stem cell therapy should be carefully evaluated in animal models and patients
with pathological pain.