bluuebunny/crossref_metadata_2025_split huggingface dataset:
Teaching biochemistry and metabolism using a YouTube channel during COVID‐19 pandemic
Authors: Tume Fernando 
Published: June 2023

AbstractYouTube is a widely recognized video‐sharing platform that students often use to search for videos that explain ideas and concepts related to their courses, seminars, or research. With the outbreak of the coronavirus pandemic, education has undergone a dramatic shift toward virtual learning, leading to a surge in the number of YouTube viewers and video creators. This article discusses the launch of a Spanish‐language YouTube channel focused on biochemistry and metabolism, which was found to be useful by the author's students enrolled in Nutritional Biochemistry course at Universidad Científica del Sur, Lima Perú. This innovation has also benefited students and teachers across Peru and other Spanish‐speaking countries. Based on this experience, teachers and professionals should be encouraged to share their knowledge on this platform, making it a reliable source of information during challenging situations.
Efficacy and safety of maintenance therapy with pamiparib versus placebo for advanced gastric cancer responding to first‐line platinum‐based chemotherapy: Phase 2 study results
Authors: Ciardiello Fortunato , Bang Yung‐Jue , Cervantes Andrés , Dvorkin Mikhail , Lopez Charles D. , Metges Jean‐Philippe , Sánchez Ruiz Antonio , Calvo Mariona , Strickland Andrew H. , Kannourakis George , Muro Kei , Kawakami Hisato , Wei Jia , Borg Christophe , Zhu Zhaoyin , Gupta Neal , Pelham Robert J. , Shen Lin 
Published: June 2023

AbstractBackgroundPoly (ADP‐ribose) polymerase (PARP) inhibitors (PARPi) are approved for the treatment of various solid tumors. In gastric cancer, genes commonly harbor mutations in the homologous recombination DNA repair pathway, potentially increasing sensitivity to PARPi. Pamiparib (BGB‐290) is a small molecule inhibitor of PARP1 and PARP2.MethodsThe PARALLEL‐303 study (NCT03427814) investigated the efficacy and safety of pamiparib 60 mg orally (PO) twice daily (BID) versus placebo PO BID as maintenance therapy in patients with inoperable locally advanced or metastatic gastric cancer that responded to platinum‐based first‐line chemotherapy. The primary endpoint of this double‐blind, randomized, global phase 2 study was progression‐free survival (PFS) (RECIST version 1.1; per investigator assessment). Secondary endpoints included overall survival (OS) and safety.ResultsIn total, 136 patients were randomized 1:1 to receive pamiparib (n = 71) or placebo (n = 65). Median PFS was numerically longer with pamiparib versus placebo but did not reach statistical significance (3.7 months [95% confidence interval (CI): 1.9, 5.3] vs. 2.1 months [95% CI: 1.9, 3.8]; hazard ratio 0.8 [95% CI: 0.5, 1.2]; p = 0.1428). Median OS was 10.2 months (95% CI: 8.7, 16.3) in the pamiparib arm versus 12.0 months (95% CI: 8.2, not estimable) in the placebo arm. Overall, 8 patients (11.3%) in the pamiparib arm and 2 patients (3.1%) in the placebo arm experienced ≥1 TEAE leading to treatment discontinuation.ConclusionsMaintenance pamiparib did not meet statistical significance for superiority versus placebo for PFS, but was well tolerated with few treatment discontinuations; no unexpected safety signals were identified.
Genomic landscape of locally advanced rectal adenocarcinoma: Comparison between before and after neoadjuvant chemoradiation and effects of genetic biomarkers on clinical outcomes and tumor response
Authors: Lee Tae Hoon , Jang Bum‐Sup , Chang Ji Hyun , Kim Eunji , Park Jeong Hwan , Chie Eui Kyu 
Published: June 2023

AbstractPurposeTo explore genomic biomarkers in rectal cancer by performing whole‐exome sequencing.Materials and MethodsPre‐chemoradiation (CRT) biopsy and post‐CRT surgical specimens were obtained from 27 patients undergoing neoadjuvant CRT followed by definitive resection. Exomes were sequenced to a mean coverage of 30×. Somatic single‐nucleotide variants (SNVs) and insertions/deletions (indels) were identified. Tumor mutational burden was defined as the number of SNVs or indels. Mutational signatures were extracted and fitted to COSMIC reference signatures. Tumor heterogeneity was quantified with a mutant‐allele tumor heterogeneity (MATH) score. Genetic biomarkers and frequently occurred copy number alterations (CNAs) were compared between pre‐ and post‐CRT specimens. Their associations with tumor regression grade (TRG) and clinical outcomes were explored.ResultsTop five mutated genes were APC, TP53, NF1, KRAS, and NOTCH1 for pre‐CRT samples and APC, TP53, NF1, CREBBP, and ATM for post‐CRT samples. Several gene mutations including RUNX1, EGFR, and TP53 in pre‐CRT samples showed significant association with clinical outcomes, but not with TRG. However, no such association was found in post‐CRT samples. Discordance of driver mutation status was found between pre‐ and post‐CRT samples. In tumor mutational burden analysis, higher number of SNVs or indels was associated with worse treatment outcomes. Six single‐base substitution (SBS) signatures identified were SBS1, SBS30, SBS29, SBS49, SBS3, and SBS44. The MATH score decreased after CRT on paired analysis. Less than half of CNAs frequent in post‐CRT samples were present in pre‐CRT samples.ConclusionPre‐ and post‐CRT samples showed different genomic landscape. Potential genetic biomarkers of pre‐CRT samples found in the current analysis call for external validation.
The role of DYNLT3 in breast cancer proliferation, migration, and invasion via epithelial‐to‐mesenchymal transition
Authors: Wang Han , Chen Xin , Jin Yanshan , Liu Tingxian , Song Yizuo , Zhu Xuejie , Zhu Xueqiong 
Published: June 2023

AbstractPurposeDYNLT3 is identified as an age‐related gene. Nevertheless, the specific mechanism of its carcinogenesis in breast tumor has not been clarified. This research aims to elucidate the role and the underlying molecular pathways of DYNLT3 on breast cancer tumorigenesis.MethodsThe differential expression of DYNLT3 among breast cancer, breast fibroids, and normal tissues, as well as in various breast cancer cell lines were detected by immunohistochemical staining, real‐time quantitative reverse transcription‐PCR and Western blotting, respectively. Additionally, the role of DYNLT3 on cell viability and proliferation were observed through cell counting kit‐8, bromodeoxyuridine, and colony formation experiments. Migratory and invasive abilities was envaulted by wound healing and Transwell methods. Apoptotic cells rate was examined by flow cytometry. Furthermore, nude mice xenograft models were established to confirm the role of DYNLT3 in tumor formation in vivo.ResultsDYNLT3 expression was highly rising in both breast cancer tissues and cells. DYNLT3 knockdown obviously suppressed cell growth, migration and invasion, and induced cell apoptosis in MDA‐MB‐231 and MCF‐7 breast cancer cells. The overexpression of DYNLT3 exerted the opposite effect in MDA‐MB‐231 cells. Moreover, DYNLT3 knockdown inhibited tumor formation in vivo. Mechanistically, an elevation of N‐cadherin and vimentin levels and a decline of E‐cadherin were observed when DYNLT3 was upregulated, which was reversed when DYNLT3 knockdown was performed.ConclusionDYNLT3 may function as a tumor‐promotor of age‐associated breast cancer, which is expected to provide experimental basis for new treatment options.
18F‐FDG PET/CT imaging of several asymptomatic skeletal muscle metastases of mixed germ cell ovarian cancer
Authors: Abdinejad Maryam , Alavi Mehrosadat 
Published: June 2023

Key Clinical MessageMetastasis to muscles caused by ovarian cancer is very rare and has a poor prognosis. Performing a whole‐body F18‐FDG PET/CT scan makes it possible to examine the whole body in one study and detected lesions in unexpected places.
A Quadruple Catalysis Enabling Intermolecular Branch‐Selective Hydroacylation of Styrenes
Authors: Takekawa Yunosuke , Nakagawa Masanari , Nagao Kazunori , Ohmiya Hirohisa 
Published: June 2023

AbstractA quadruple N‐heterocyclic carbene/cobalt/photoredox/Brønsted base catalysis to realize branch‐selective hydroacylation of styrenes with aromatic and aliphatic aldehydes is demonstrated. This protocol allows access to branched ketones from readily available materials in an atom‐economical manner. The quadruple catalysis can transfer a formyl hydrogen of aldehydes as a hydrogen radical equivalent onto the terminal carbon of an alkene by controlled electron and proton transfers.
Atmospheric Degradation and Performance Recovery of Two‐Dimensional MoS2 Field Effect Transistor†
Authors: Sun Lifei , Lu Zhixing , Xu Rui , Li Zhewei , Xu Guanchen , Chen Fengen , Cheng Zhihai , Jiao Liying 
Published: June 2023

Comprehensive SummaryTwo‐dimensional (2D) transition metal dichalcogenides (TMDCs) showed great potentials in 2D nanoelectronic devices due to their abundant and unique properties. The performance stability of the 2D TMDCs devices turns into one of the keys for their practical applications but has been rarely explored. Here, we investigated stability of MoS2 devices in ambient condition and contributed the device performance degradation to the surface oxidation of the contact metals with low work function, which increased the contact barrier and hindered the electron injection. We developed a new approach to recover the performance of the aged devices through the selective doping of contacts with organolithium, which prolonged the lifetime of MoS2 devices. Our work not only provides important insights into the stability of 2D TMDCs devices, but also opens up a new avenue for optimizing the performance of 2D MoS2 devices.
Enhanced lactic acid production by Lactobacillus rhamnosus ATCC 7469 through simultaneous saccharification and fermentation from household food waste
Authors: Song Liang , Liu Shiyu , Yang Donghai , Liu Rui , Dai Xiaohu 
Published: June 2023

AbstractA large amount of household food waste (HFW) is generated yearly and may negatively impact the environment and human health. The recovery of value‐added chemicals lactic acid (LA) from HFW through anaerobic treatment is a promising approach. However, it remains a challenge to increase the LA yield. In this paper, the LA production from HFW was enhanced by Lactobacillus rhamnosus ATCC 7469 through simultaneous saccharification and fermentation (SSF). The results showed that the mixed commercial enzymes of plant enzymes (E1), animal proteases (E2), and glucoamylase (E3) were more conducive to LA production than those used separately. Compared to the separate hydrolysis and fermentation (SHF), the LA production increased by 14% in the SSF, and the glucose concentration showed different change rules. Additionally, an average LA concentration of 47.7 g L–1 was obtained by repeated batch fermentation through SSF. However, the maximum LA concentration decreased with the fermentation ongoing. The trend of decreasing LA production could be improved by repeated batch fermentation with supplementary inoculation.
Diagnosis of salivary gland tumors: Does the triple diagnosis method have value?
Authors: Preston Peace , Esebua Magda , Layfield Lester J. 
Published: June 2023

AbstractBackgroundAppropriate clinical management of salivary gland lesions requires a determination as to whether a salivary gland nodule is benign or malignant. Approximately three‐quarters of all salivary gland nodules represent benign neoplasms. Separation of salivary gland carcinomas from benign lesions can be diagnostically challenging. The Milan System for Reporting Salivary Gland Cytopathology recommends the correlation of cytologic diagnoses with imaging and clinical findings creating a diagnostic triplet. How often the “Triple Diagnosis” method is used and its accuracy in separating salivary gland nodules into benign and malignant groups are unknown.MethodsAn electronic records search of cytology files at the University of Missouri was performed for fine needle aspirates of the salivary gland obtained between September 2018 and August 2022. Chart review was performed for preoperative clinical and imaging diagnoses. Diagnostic “Triplets” constructed from cytologic, clinical, and imaging diagnoses were correlated with final surgical pathology diagnosis.ResultsOne hundred and thirty‐six FNAs were identified. Eighty‐seven cases had preoperative imaging with 52 of these cases having clinical diagnoses. Due to the lack of a definitive clinical or imaging diagnosis for a nodule as benign or malignant, only 12 (23%) cases had definitive “Triplets.” Nine (17%) “Triplets” were benign and three (6%) were malignant. Accuracy of concordant triplets was 100% for the prediction of malignancy and 89% for the prediction of a benign result as determined by final histologic diagnoses.ConclusionWhile highly accurate in predicting the benign or malignant nature of a salivary gland nodule, concordant triplets made up only 23% of cases limiting their clinical utility.
Xanthatin induce DDP‐resistance lung cancer cells apoptosis through regulation of GLUT1 mediated ROS accumulation
Authors: Liu Yunxiao , Zhang Xinge , Cheng Fenting , Cao Wei , Geng Yadi , Chen Zhaolin , Wei Wei , Zhang Lei 
Published: June 2023

AbstractChemoresistance to cisplatin (DDP) therapy is a major obstacle that needs to be overcome in treating lung cancer patients. Xanthatin has been reported to exhibit an antitumor effect on various cancers, but the function of xanthatin in DDP‐resistance lung cancer remains unclear. The study aimed to explore the effect and mechanisms of xanthatin on proliferation, apoptosis, and migration in DDP‐resistance lung cancer cells. In the present study, xanthatin suppresses the expression of glucose transporter 1 (GLUT1), attenuates the pentose phosphate pathway (PPP), and causes ROS accumulation and apoptosis, thereby mitigating the antioxidative capacity in DDP‐resistance cells. Previous studies have shown that GLUT1 is associated with resistance to platinum drugs. We found that GLUT1 was significantly increased in the DDP‐resistant lung cancer cell line compared to the parental cell line, and xanthatin significantly downregulated GLUT1 expression in DDP‐resistant lung cancer cells. Notably, overexpression of GLUT1 significantly reduced the production of ROS and increased cellular NADPH/NADP+ and GSH/GSSG ratios. Thus, these results suggest that xanthatin induces DDP‐resistance lung cancer cells apoptosis through regulation of GLUT1‐mediated ROS accumulation. These findings might provide a possible strategy for the clinical treatment of DDP‐resistant lung cancer.
A framework to identify functional interactors that contribute to disrupted early retinal development in Vsx2 ocular retardation J mice
Authors: Leung Amanda M. , Rao Mahesh B. , Raju Nathan , Chung Minh , Klinger Allison , Rowe DiAnna J. , Li Xiaodong , Levine Edward M. 
Published: June 2023

AbstractBackgroundA goal of developmental genetics is to identify functional interactions that underlie phenotypes caused by mutations. We sought to identify functional interactors of Vsx2, which when mutated, disrupts early retinal development. We utilized the Vsx2 loss‐of‐function mouse, ocular retardation J (orJ), to assess interactions based on principles of positive and negative epistasis as applied to bulk transcriptome data. This was first tested in vivo with Mitf, a target of Vsx2 repression, and then to cultures of orJ retina treated with inhibitors of Retinoid‐X Receptors (RXR) to target Rxrg, an up‐regulated gene in the orJ retina, and gamma‐Secretase, an enzyme required for Notch signaling, a key mediator of retinal proliferation and neurogenesis.ResultsWhereas Mitf exhibited robust positive epistasis with Vsx2, it only partially accounts for the orJ phenotype, suggesting other functional interactors. RXR inhibition yielded minimal evidence for epistasis between Vsx2 and Rxrg. In contrast, gamma‐Secretase inhibition caused hundreds of Vsx2‐dependent genes associated with proliferation to deviate further from wild‐type, providing evidence for convergent negative epistasis with Vsx2 in regulating tissue growth.ConclusionsCombining in vivo and ex vivo testing with transcriptome analysis revealed quantitative and qualitative characteristics of functional interaction between Vsx2, Mitf, RXR, and gamma‐Secretase activities.
Breastfeeding and pumping as maladaptive weight control behaviors
Authors: Bailey‐Straebler Suzanne M. , Susser Leah C. , Cooper Zafra 
Published: June 2023

AbstractIt is well recognized by the general public that breastfeeding expends calories. In our clinical practice, a number of postpartum women with a history of or a current eating disorder (ED) report using breastfeeding and/or pumping breast milk to influence their body shape and weight. This appears to be either a form of weight control behavior or, in some cases, a compensation for perceived overeating or binge eating. Breastfeeding and pumping have not generally been identified as maladaptive weight control behaviors, nor have they been a subject of research to date. We suggest that this practice should be investigated to determine how common it is, its potential role in maintaining EDs or contributing to relapse in the postpartum period, and to better understand other potential harms it may cause to both the mother and infant/child.Public significanceBreastfeeding and pumping may be used as maladaptive methods of weight control by women with EDs. This behavior is under‐recognized in clinical practice and has received little research attention. We argue that maladaptive breastfeeding and pumping warrant further investigation, as the behaviors may play an important role in maintaining an ED or in contributing to relapse during the postpartum period and may also indirectly harm the infant/child.
Nitraza‐/Oxa‐Propylene‐ and Hydrazonemethylene‐Bridged 1,2,4‐Nitraminotriazoles and Selected Salts
Authors: Harter Alexander G. , Klapötke Thomas M. , Krumm Burkhard , Lechner Jasmin T. , Riedelsheimer Christian 
Published: June 2023

AbstractTwo new bridged nitraminotriazoles with bridging oxapropylene and nitrazapropylene moieties were synthesized, and converted into several salts, as well as from the hydrazonemethylene bridged nitraminotriazole. All compounds were fully characterized by NMR and IR spectroscopy, elemental analysis as well as differential thermal analysis. The sensitivity towards friction and impact were determined according to BAM standard technics and the energetic properties were calculated by using the EXPLO5 computer code. The neutral compounds as well as the various salts were examined in terms of their physicochemical properties and detonation performance to each other and compared to the commonly used secondary explosive RDX.
Assessing the efficacy of water management and wheat straw addition in mitigating methane emissions from rice paddy fields
Authors: Rassaei Farzad 
Published: June 2023

AbstractTo improve the quality of soil, agriculture typically uses organic additions like wheat straw (WS). Water management (WM) methods such as alternative wetness and dryness irrigation (AWDI) in paddy rice farming can increase water use efficiency and reduce greenhouse gas (GHG) emissions. In this study, a randomized complete block design (RCBD) pot greenhouse experiment was conducted to assess the effects of WM (CFI (continued flooding irrigation) and AWDI), WS (15.0 and 30.0 ton ha−1), and their interaction (WS*WM) on CH4emissions and rice yield in a paddy calcareous soil cultivated with rice. The largest CH4emission occurred 30 and 70 days following rice planting. Single applications of WS at 15.0 and 30.0 ton ha−1significantly increased CH4emissions by 19.60% and 68.70% (P < 0.01), respectively, compared to the Control. Compared to CFI, the AWDI reduced CH4emissions by 31.20% (P < 0.01). The correlations between AWDI and CH4emissions in days after rice planting were significantly negative at the 0.01 level based on Pearson correlation. Adding WS in the AWDI significantly decreased CH4emissions by 27.70 and 25.64% in 15.0 and 30.0 ton ha−1added WS (P < 0.05), respectively, compared to sole WS. Both rates of WS resulted in a considerable increase in biomass and rice grain compared to the Control, while the AWDI had no significant effect on biomass and grain yield. Our data showed significant interaction between AWDI and WS on biomass. Our study shows that the AWDI saved 18.50% water compared to the CFI.
Clean printing of cotton fabrics using novel environmental‐friendly phthalocyanine‐monoazo reactive dyes
Authors: Hu Liu , Yu Yingsong , Han Huayu , Zhang Shangkun , Hu Yi , Song Xiyu 
Published: June 2023

AbstractApplication of reactive dyes with low fixation in textile coloring field has caused discharges of colored wastewater and waste of resources. In this work, to reduce the discharge and improve the utilization, three novel reactive dyes (D1, D2, D3) with high reactivity based on copper phthalocyanine‐azo skeleton were designed and synthesized, and the structures were characterized by UV–vis, FT‐IR, and 1H NMR. All these dyes had large molar extinction coefficients, and they presented violet, brown, and dark‐violet colors. The printing performances on cotton fabrics of the dyes were investigated. Results show that the printing properties of the dyes on fabrics were not obviously affected by alkaline concentration, urea concentration, and steaming time. In addition, the printing process leaded to colorless wastewater, and the fixations on cotton fabrics of D1, D2, and D3 were all close to 100%, illustrating high utilization of the novel dyes. The design and application of this kind of reactive dye could provide a significant approach for reducing the discharge of colored wastewater and saving energy.
Experimental investigation in the selection of phase change materials based on thermal stratification effect in thermal energy storage system
Authors: Christopher Sathiya Satchi , Vikram Muthuraman Ponrajan , Bharathi Balavadivel Raja 
Published: June 2023

AbstractThis study aims to evaluate the effects of thermal stratification on thermal energy storage (TES) systems during the charging process and choose suitable phase change materials (PCMs) for various layers. The research revealed that fiberglass insulation of 60 mm thick significantly decreased natural convection losses and increased the TES system's potential for energy recovery. For constant heat load, the charging time increased with increasing HTF inlet temperature, but remained constant for the first TES layer at a specified flow rate, resulting in increased storage capacity with minimal heat loss to the surroundings. The solar collector efficiency was shown to be dependent on solar radiation and collector efficiency, with peak efficiency seen at specified periods. The lowest melting point of PCMs at each layer 68°C, 62.7°C, 57°C, and 50°C was chosen based on temperature graphs. For comparing stratification behavior, the “MIX” number versus non‐dimensional charging time plot was effective. Additional experiments are needed to assess the selected PCMs' charging and discharging properties.