bluuebunny/crossref_metadata_2025_split huggingface dataset:
The small and large intestine contain related mesenchymal subsets that derive from embryonic Gli1+ precursors
Authors: Pærregaard Simone Isling , Wulff Line , Schussek Sophie , Niss Kristoffer , Mörbe Urs , Jendholm Johan , Wendland Kerstin , Andrusaite Anna T. , Brulois Kevin F. , Nibbs Robert J. B. , Sitnik Katarzyna , Mowat Allan McI , Butcher Eugene C. , Brunak Søren , Agace William W. 
Published: April 2023

AbstractThe intestinal lamina propria contains a diverse network of fibroblasts that provide key support functions to cells within their local environment. Despite this, our understanding of the diversity, location and ontogeny of fibroblasts within and along the length of the intestine remains incomplete. Here we show that the small and large intestinal lamina propria contain similar fibroblast subsets that locate in specific anatomical niches. Nevertheless, we find that the transcriptional profile of similar fibroblast subsets differs markedly between the small intestine and colon suggesting region specific functions. We perform in vivo transplantation and lineage-tracing experiments to demonstrate that adult intestinal fibroblast subsets, smooth muscle cells and pericytes derive from Gli1-expressing precursors present in embryonic day 12.5 intestine. Trajectory analysis of single cell RNA-seq datasets of E12.5 and adult mesenchymal cells suggest that adult smooth muscle cells and fibroblasts derive from distinct embryonic intermediates and that adult fibroblast subsets develop in a linear trajectory from CD81+ fibroblasts. Finally, we provide evidence that colonic subepithelial PDGFRαhi fibroblasts comprise several functionally distinct populations that originate from an Fgfr2-expressing fibroblast intermediate. Our results provide insights into intestinal stromal cell diversity, location, function, and ontogeny, with implications for intestinal development and homeostasis.
Error-related signaling in nucleus accumbens D2 receptor-expressing neurons guides inhibition-based choice behavior in mice
Authors: Nishioka Tadaaki , Attachaipanich Suthinee , Hamaguchi Kosuke , Lazarus Michael , de Kerchove d’Exaerde Alban , Macpherson Tom , Hikida Takatoshi 
Published: April 2023

AbstractLearned associations between environmental cues and the outcomes they predict (cue-outcome associations) play a major role in behavioral control, guiding not only which responses we should perform, but also which we should inhibit, in order to achieve a specific goal. The encoding of such cue-outcome associations, as well as the performance of cue-guided choice behavior, is thought to involve dopamine D1 and D2 receptor-expressing medium spiny neurons (D1-/D2-MSNs) of the nucleus accumbens (NAc). Here, using a visual discrimination task in male mice, we assessed the role of NAc D1-/D2-MSNs in cue-guided inhibition of inappropriate responding. Cell-type specific neuronal silencing and in-vivo imaging revealed NAc D2-MSNs to contribute to inhibiting behavioral responses, with activation of NAc D2-MSNs following response errors playing an important role in optimizing future choice behavior. Our findings indicate that error-signaling by NAc D2-MSNs contributes to the ability to use environmental cues to inhibit inappropriate behavior.
Geometric fluctuation of conformal Hilbert spaces and multiple graviton modes in fractional quantum Hall effect
Authors: Yuzhu Wang , Bo Yang 
Published: April 2023

AbstractNeutral excitations in fractional quantum Hall (FQH) fluids define the incompressibility of topological phases, a species of which can show graviton-like behaviors and are thus called the graviton modes (GMs). Here, we develop the microscopic theory for multiple GMs in FQH fluids and show explicitly that they are associated with the geometric fluctuation of well-defined conformal Hilbert spaces (CHSs), which are hierarchical subspaces within a single Landau level, each with emergent conformal symmetry and continuously parameterized by a unimodular metric. This leads to several statements about the number and the merging/splitting of GMs, which are verified numerically with both model and realistic interactions. We also discuss how the microscopic theory can serve as the basis for the additional Haldane modes in the effective field theory description and their experimental relevance to realistic electron-electron interactions.
Oxidative dissolution of (U,Ce)O2 materials in aqueous solutions containing H2O2
Authors: Montaigne Théo , Szenknect Stéphanie , Broudic Véronique , Imbert Paul-Henri , Tocino Florent , Martin Christelle , Miserque Frédéric , Jégou Christophe , Dacheux Nicolas 
Published: April 2023

AbstractHomogeneous and heterogeneous U1-xCexO2 (with 0≤ x≤ 0.25) materials were prepared via wet and dry chemistry routes, respectively before being submitted to dynamic leaching experiments. The feeding solution containing 0.20 mmol.L−1 H2O2 was kept under air and renewed to guarantee the stability of H2O2 during the experiment. Normalized alteration rates were determined from U concentration in the leachates. For homogeneous (U,Ce)O2 materials, the dissolution rate was divided by a factor of 3 when increasing the Ce content from 0.08 to 0.25. Surface characterizations revealed that studtite precipitated all over UO2 pellet surface and only on the UO2 grains of heterogeneous U0.92Ce0.08O2 samples. The behaviour of this heterogeneous material was similar to that observed for (U,Pu)O2 in the same conditions, which revealed the reliability of cerium as a plutonium analogue.
The potential of bamboo seeds for natural biofortification of dietary zinc and iron
Authors: Hu Qifang , Wang Rong , Hu Lin , Chen Rong , Yu Xuejun , Shao Ji Feng 
Published: April 2023

AbstractMoso bamboo has been shown to accumulate high concentrations of iron and zinc in the seeds. However, the bioavailablity of iron and zinc in bamboo seeds is poorly understood. Here, we evaluated the bioaccessibility and bioavailability of iron and zinc in bamboo seeds by using an in vitro digestion protocol. Our evaluations revealed that values of bioaccessibility and bioavailability of iron were 25 and 21 mg kg−1in bamboo seeds which were 1.6- and 1.7- fold higher than in rice, respectively. Also, values of bioaccessibility and bioavailability of zinc were 20 and 13 mg kg−1in bamboo seeds which were 1.9- and 2.6- fold higher than in rice, respectively. Boiling process reduced both the bioaccessibility and bioavailability of iron and zinc. In addition, phytic acid concentration in bamboo seeds was only 0.42 times higher than in rice. By contrast, the tannins concentration in bamboo seeds was 2.2 times higher than in rice. Cellular localization results showed that iron and zinc were mainly concentrated in the embryo and the aleurone layer. These results clearly suggest that Moso bamboo seeds are rich in iron and zinc and have potential as a food for iron and zinc biofortification.
On enabling collaborative non-intrusive load monitoring for sustainable smart cities
Authors: Shi Yunchuan , Li Wei , Chang Xiaomin , Yang Ting , Sun Yaojie , Zomaya Albert Y. 
Published: April 2023

AbstractImproving energy efficiency is a crucial aspect of building a sustainable smart city and, more broadly, relevant for improving environmental, economic, and social well-being. Non-intrusive load monitoring (NILM) is a computing technique that estimates energy consumption in real-time and helps raise energy awareness among users to facilitate energy management. Most NILM solutions are still a single machine approach and do not fit well in smart cities. This work proposes a model-agnostic hybrid federated learning framework to collaboratively train NILM models for city-wide energy-saving applications. The framework supports both centralised and decentralised training modes to provide a cluster-based, customisable and optimal learning solution for users. The proposed framework is evaluated on a real-world energy disaggregation dataset. The results show that all NILM models trained in our proposed framework outperform the locally trained ones in accuracy. The results also suggest that the NILM models trained in our framework are resistant to privacy leakage.
Clinical and microbiological characteristics of bloodstream infection caused by Klebsiella pneumoniae harboring rmpA in Japanese adults
Authors: Kikuchi Shota , Kosai Kosuke , Ota Kenji , Mitsumoto-Kaseida Fujiko , Sakamoto Kei , Hasegawa Hiroo , Izumikawa Koichi , Mukae Hiroshi , Yanagihara Katsunori 
Published: April 2023

AbstractWe investigated the clinical features of bloodstream infections (BSIs) caused by Klebsiella pneumoniae harboring rmpA and molecular characteristics of the bacteria. We retrospectively investigated adult patients with K. pneumoniae BSI from January 2010 to March 2021 at Nagasaki University Hospital. A matched case–control study in a 1:3 ratio was conducted to clarify the clinical and bacterial characteristics of BSI caused by rmpA-positive K. pneumoniae compared with those caused by rmpA-negative isolates. Antimicrobial susceptibility testing and multilocus sequence typing (MLST) were performed for rmpA-positive isolates. The rmpA was detected in 36 (13.4%) of the 268 isolates. Of these 36 isolates, 31 (86.1%) harbored iucA and 35 (97.2%) each possessed peg-344 and iroB; capsular types were identified as K1 in 9 (25.0%) and K2 in 10 isolates (27.8%). Contrarily, of the 108 rmpA-negative isolates, which were matched for case–control studies, 5 isolates (4.6%) harbored iucA and 1 (0.9%) each possessed peg-344 and iroB; 2 (1.9%) and 3 isolates (2.8%) had K1 and K2 capsular types, respectively. Among the rmpA-positive isolates, ST23/K1 (eight isolates) was the most frequent, followed by ST412/non-K1/K2 (seven isolates), ST86/K2 (five isolates), and ST268/non-K1/K2 (four isolates). In a multivariate analysis using clinical factors, liver abscess positively correlated with rmpA-positive isolates, whereas biliary tract infection and use of anticancer drugs negatively correlated with rmpA-positive isolates in patients with K. pneumoniae BSI. Considering the correlation between rmpA-positive isolates and clinical features, rmpA can be used as a marker for understanding the pathophysiology of K. pneumoniae BSI.
Exploration on ability of printable modified papers for the application in heat sublimation transfer printing of polyester fabric
Authors: Adel Abeer M. , Ahmed Nivin M. , Diab Mohamed A. , El-Shall Fatma N. , El-Shinnawy Nabila 
Published: April 2023

AbstractIn this work heat transfer papers were loaded with a new core–shell pigment based on precipitating thin shell of titanium dioxide on a core of rice husk silica ash TiO2/RHSA to be applied in dye sublimation printing of textile fabrics. Besides, 0.1% (w/w) cationic polyacrylamide (CPAM) and 1% (w/w) bentonite (Bt) were also added sequentially to improve drainage and filler retention of the paper hand-sheets made from bleached kraft bagasse pulps. The effect of the new core–shell pigment on the mechanical and barrier properties, thermal stability and surface morphology of modified paper sheets were investigated. In addition, the study of transfer printability and ease of dye release from paper to fabric in this heat transfer printing of polyester fabrics using silk-screen printing under different transfer parameters were studied. Also, fastness measurements including washing, light and perspiration of printing polyester fabric were also estimated.
The screening method for use of wild pheasant feathers in the monitoring of environmental pollution with heavy metals
Authors: Tajchman Katarzyna , Drabik Kamil , Ukalska-Jaruga Aleksandra , Janiszewski Paweł , Spustek Damian , Wengerska Karolina 
Published: April 2023

AbstractIt has been shown that some species of birds, especially herbivorous and territorial ones, are more sensitive to the effects of toxic substances compared to mammals. This allows for taking integrated actions in the area of environmental protection and monitoring in a holistic sense (at various trophic levels). Therefore, this study aimed to assess the possibility of using pheasant feathers (Phasianuscolchicus) as a potential bioindicator ofenvironmental contamination, and thus to determine the concentration of heavy metals (lead—Pb, arsenic—As, cadmium—Cd, chromium—Cr, nickel—Ni, and zinc—Zn) in the analyzed tissue of animals inhabiting the forest districts of the Lubartów, Tomaszów, Skierniewice, and Ostrowiec Świętokrzyski areas. The chemical analysis used to determine the concentration of toxic elements in pheasant feathers was carried out by inductively coupled plasma mass spectrometry. The highest concentrations of Cr and Zn were found in birds from the Lubartów Forest District (1.93 mg/kg and 120.63 mg/kg, respectively), As and Ni in the Tomaszów Forest District (0.55 mg/kg and 1.60 mg/kg, respectively), Cd in the Lubartów Forest District and Skierniewice (0.04 mg/kg), and Pb in the Skierniewice Forest Distict (6.79 mg/kg). The observed results were strongly related to soil contamination and urbanization index, as key environmental factors which significantly determine the metal content in pheasant feathers. Therefore, proposed non-invasive measurements of the elemental composition of feathers of birds living in specific areas may be an important indicator of environmental pollution in relation to the high impact of anthropopressure.
Influence of lateral single jets for thermal protection of reentry nose cone with multi-row disk spike at hypersonic flow: computational study
Authors: Shi Yunbin , Cheng Qiong , Alizadeh As’ad , Yan Hongbo , Choubey Gautam , Fallah K. , Shamsborhan Mahmoud 
Published: April 2023

AbstractThe main challenge for the advancement of current high-speed automotives is aerodynamic heating. In this study, the application of lateral jet for thermal protection of the high-speed automotives is extensively studied. The simulation of the lateral coolant jet is done via Computational fluid dynamic at high-velocity condition. Finding optimum jet configuration for reduction of the aerodynamic heating is the main goal of this research. Two different coolant jets (Helium and Carbon dioxide) are investigated as coolant jet and flow study and fuel penetration mechanism are fully presented. In addition, the thermal load on the main body of nose cone is compared for different configurations. Our results specify that the injection of lateral jet near the tip of spike is effective for thermal protection of main body via deflection of bow shock. Also, Carbon dioxide jet with lower diffusivity is more effective for the protection of forebody with multi-row disk from sever aerodynamic heating.
Automated cornea diagnosis using deep convolutional neural networks based on cornea topography maps
Authors: Fassbind Benjamin , Langenbucher Achim , Streich Andreas 
Published: April 2023

AbstractCornea topography maps allow ophthalmologists to screen and diagnose cornea pathologies. We aim to automatically identify any cornea abnormalities based on such cornea topography maps, with focus on diagnosing keratoconus. To do so, we represent the OCT scans as images and apply Convolutional Neural Networks (CNNs) for the automatic analysis. The model is based on a state-of-the-art ConvNeXt CNN architecture with weights fine-tuned for the given specific application using the cornea scans dataset. A set of 1940 consecutive screening scans from the Saarland University Hospital Clinic for Ophthalmology was annotated and used for model training and validation. All scans were recorded with a CASIA2 anterior segment Optical Coherence Tomography (OCT) scanner. The proposed model achieves a sensitivity of 98.46% and a specificity of 91.96% when distinguishing between healthy and pathological corneas. Our approach enables the screening of cornea pathologies and the classification of common pathologies like keratoconus. Furthermore, the approach is independent of the topography scanner and enables the visualization of those scan regions which drive the model’s decisions.
Machine learning reveals limited contribution of trans-only encoded variants to the HLA-DQ immunopeptidome
Authors: Nilsson Jonas Birkelund , Kaabinejadian Saghar , Yari Hooman , Peters Bjoern , Barra Carolina , Gragert Loren , Hildebrand William , Nielsen Morten 
Published: April 2023

AbstractHuman leukocyte antigen (HLA) class II antigen presentation is key for controlling and triggering T cell immune responses. HLA-DQ molecules, which are believed to play a major role in autoimmune diseases, are heterodimers that can be formed as both cis and trans variants depending on whether the α- and β-chains are encoded on the same (cis) or opposite (trans) chromosomes. So far, limited progress has been made for predicting HLA-DQ antigen presentation. In addition, the contribution of trans-only variants (i.e. variants not observed in the population as cis) in shaping the HLA-DQ immunopeptidome remains largely unresolved. Here, we seek to address these issues by integrating state-of-the-art immunoinformatics data mining models with large volumes of high-quality HLA-DQ specific mass spectrometry immunopeptidomics data. The analysis demonstrates highly improved predictive power and molecular coverage for models trained including these novel HLA-DQ data. More importantly, investigating the role of trans-only HLA-DQ variants reveals a limited to no contribution to the overall HLA-DQ immunopeptidome. In conclusion, this study furthers our understanding of HLA-DQ specificities and casts light on the relative role of cis versus trans-only HLA-DQ variants in the HLA class II antigen presentation space. The developed method, NetMHCIIpan-4.2, is available at https://services.healthtech.dtu.dk/services/NetMHCIIpan-4.2.
Lunar gravity prevents skeletal muscle atrophy but not myofiber type shift in mice
Authors: Hayashi Takuto , Fujita Ryo , Okada Risa , Hamada Michito , Suzuki Riku , Fuseya Sayaka , Leckey James , Kanai Maho , Inoue Yuri , Sadaki Shunya , Nakamura Ayano , Okamura Yui , Abe Chikara , Morita Hironobu , Aiba Tatsuya , Senkoji Teruhiro , Shimomura Michihiko , Okada Maki , Kamimura Daisuke , Yumoto Akane , Muratani Masafumi , Kudo Takashi , Shiba Dai , Takahashi Satoru 
Published: April 2023

AbstractSkeletal muscle is sensitive to gravitational alterations. We recently developed a multiple artificial-gravity research system (MARS), which can generate gravity ranging from microgravity to Earth gravity (1 g) in space. Using the MARS, we studied the effects of three different gravitational levels (microgravity, lunar gravity [1/6 g], and 1 g) on the skeletal muscle mass and myofiber constitution in mice. All mice survived and returned to Earth, and skeletal muscle was collected two days after landing. We observed that microgravity-induced soleus muscle atrophy was prevented by lunar gravity. However, lunar gravity failed to prevent the slow-to-fast myofiber transition in the soleus muscle in space. These results suggest that lunar gravity is enough to maintain proteostasis, but a greater gravitational force is required to prevent the myofiber type transition. Our study proposes that different gravitational thresholds may be required for skeletal muscle adaptation.
RAB35 is required for murine hippocampal development and functions by regulating neuronal cell distribution
Authors: Maejima Ikuko , Hara Taichi , Tsukamoto Satoshi , Koizumi Hiroyuki , Kawauchi Takeshi , Akuzawa Tomoko , Hirai Rika , Kobayashi Hisae , Isobe Inoya , Emoto Kazuo , Kosako Hidetaka , Sato Ken 
Published: April 2023

AbstractRAB35 is a multifunctional small GTPase that regulates endocytic recycling, cytoskeletal rearrangement, and cytokinesis. However, its physiological functions in mammalian development remain unclear. Here, we generatedRab35-knockout mice and found that RAB35 is essential for early embryogenesis. Interestingly, brain-specificRab35-knockout mice displayed severe defects in hippocampal lamination owing to impaired distribution of pyramidal neurons, although defects in cerebral cortex formation were not evident. In addition,Rab35-knockout mice exhibited defects in spatial memory and anxiety-related behaviors. Quantitative proteomics indicated that the loss of RAB35 significantly affected the levels of other RAB proteins associated with endocytic trafficking, as well as some neural cell adhesion molecules, such as contactin-2. Collectively, our findings revealed that RAB35 is required for precise neuronal distribution in the developing hippocampus by regulating the expression of cell adhesion molecules, thereby influencing spatial memory.
A data-driven crop model for maize yield prediction
Authors: Chang Yanbin , Latham Jeremy , Licht Mark , Wang Lizhi 
Published: April 2023

AbstractAccurate estimation of crop yield predictions is of great importance for food security under the impact of climate change. We propose a data-driven crop model that combines the knowledge advantage of process-based modeling and the computational advantage of data-driven modeling. The proposed model tracks the daily biomass accumulation process during the maize growing season and uses daily produced biomass to estimate the final grain yield. Computational studies using crop yield, field location, genotype and corresponding environmental data were conducted in the US Corn Belt region from 1981 to 2020. The results suggest that the proposed model can achieve an accurate prediction performance with a 7.16% relative root-mean-square-error of average yield in 2020 and provide scientifically explainable results. The model also demonstrates its ability to detect and separate interactions between genotypic parameters and environmental variables. Additionally, this study demonstrates the potential value of the proposed model in helping farmers achieve higher yields by optimizing seed selection.
Search for ferromagnetism in Mn-doped lead halide perovskites
Authors: Sajedi Maryam , Luo Chen , Siemensmeyer Konrad , Krivenkov Maxim , Chen Kai , Taylor James M. , Flatken Marion A. , Radu Florin , Rader Oliver 
Published: April 2023

AbstractLead halide perovskites are new key materials in various application areas such as high efficiency photovoltaics, lighting, and photodetectors. Doping with Mn, which is known to enhance the stability, has recently been reported to lead to ferromagnetism below 25 K in methylammonium lead iodide (MAPbI3) mediated by superexchange. Two most recent reports confirm ferromagnetism up to room temperature but mediated by double exchange between Mn2+ and Mn3+ ions. Here we investigate a wide concentration range of MAMnxPb1−xI3 and Mn-doped triple-cation thin films by soft X-ray absorption, X-ray magnetic circular dichroism, and quantum interference device magnetometry. The X-ray absorption lineshape shows clearly an almost pure Mn2+ configuration, confirmed by a sum-rule analysis of the dichroism spectra. A remanent magnetization is not observed down to 2 K. Curie-Weiss fits to the magnetization yield negative Curie temperatures. All data show consistently that significant double exchange and ferromagnetism do not occur. Our results show that Mn is not suitable for creating ferromagnetism in lead halide perovskites.