bluuebunny/crossref_metadata_2025_split huggingface dataset:
Feasibility of Growing Selected Vegetables in Hanging Sac With Different Levels of Manure
Authors: Sultana R , Dash PK , Das S , Mannan MA , Gomasta J 
Published: January 2023

Vegetable production in the waterlogged condition is the main challenge in Southwest Bangladesh and to address the problem the present investigation was undertaken to determine the prospect and profitability of hanging sack vegetable cultivation in the waterlogged condition under various levels of organic matter. The investigation comprised three of vegetables such as Brinjal, Tomato, and Okra in hanging sacs under three soil and organic matter combinations at Germplasm Center of Agrotechnology Discipline, Khulna University Bangladesh from November 2018 to March 2019. Three soil+cowdung combinations were T0= 100% soil, T1= 50% soil+50% cowdung, T2= 75% soil+ 25% cowdung, T3=25% soil+ 75% cowdung replicated seven times to produce the less erroneous results. Brinjal in the hanging sac had significantly better biological yield (28.92 t/ha), net return (451,000Tk./ha)and benefit-cost ratio (BCR) (2.08)over okra or tomato when the sac was filled with 75% cowdung + 25% soil. The higher price plus advanced yield contributed to enhancing the profitability of brinjal. These results will inform decision-making about cropping system modification that can be adopted by vegetable growers of Southwest Bangladesh to considerably reduce fallow waterlogged areas and enhanced vegetable production in waterlogged conditions.
Bangladesh J. Agril. Res. 46(1): 35-42, March 2021
Effect of Soil Moisture Level on Yield and Nutrient Balance Sheet of Black Cumin
Authors: Yousuf MN , Karim AJMS , Solaiman ARM , Islam MS , Zakaria M 
Published: January 2023

A field experiment was conducted at Bangabandhu Sheikh Mujibur Rahman Agricultural University, Gazipur during rabi season of 2016-17 to evaluate the effects of soil moisture levels on black cumin (BARI Kalozira-1). The study site belongs to Shallow Red-Brown Terrace Soil of Salna series under AEZ-28 (Madhupur Tract). The experiment was set up in randomized complete block design with three replications, comprised five treatments assoil moisture levels: irrigation at 90%, 80%, 70%, 60% and 50% field capacity. The required number and amount of irrigation were 11 and 113.1mm, 8 and 108.1mm, 6 and 104.2mm, 4 and 99.4mm and 0 and 22.4mm in irrigation at 90%, 80%, 70%, 60% and 50% field capacity, respectively. The maximum seed yield (1243.0 kg ha-1) was recorded under the highest moisture regime in irrigation at 90% field capacity of soil moisture. The minimum yield (165.5 kg ha-1) was noted at 50% field capacity (where no irrigation was required). Net nutrient balance of N, K and S showed negative balance but P was positive. The maximum water use efficiency (10.51 kg ha-1 mm-1) for yield was noted with irrigation at 90% field capacity, which may be recommended for black cumin cultivation in the study area. The cost-benefit analyses also indicate that the maximum net income (Tk. 123503.00) of black cumin cultivation has been achieved by maintaining the highest soil moisture through irrigating the crop at 90% of field capacity.
Bangladesh J. Agril. Res. 46(1): 43-61, March 2021
Screening of Betelvine Cultivars Against Sclerotium Rolfsii Causing Foot and Root Rot Disease
Authors: Rahman MH , Islam MR , Aminuzzaman FM , Patwary MMA , Nasim MFA 
Published: January 2023

Thirteen betelvine cultivars (PB 001 to PB 013) collected from different locations of Bangladesh showed remarkable variations in disease reaction to foot and root rot caused by Sclerotium rolfsii. The lowest incubation period 8 days was required for the cultivars PB 005 (BARI line), PB 006 (Misti pan), PB 009 (BARI Pan-1) and PB 010 (Bangla pan) and the highest incubation period 22 days was required for the cultivars PB 001 (Laldingi pan) and PB 013 (Gayasur pan) for appearance of 1st disease symptoms. The disease incidence ranged 8.33 to 100% at 30 days after inoculation. The lowest disease incidence was recorded in PB 001 (Laldingi pan), while the highest disease incidence was recorded from PB 002, PB 003 (Chalitaguti pan), PB 004 (Sanchi pan), PB 005, PB 006 (Misti pan), PB 007, PB 008, PB 009 (BARI Pan-1), PB 010 (Bangla pan) and PB 012 (Bhabna pan) followed by PB 011 (Jhal pan) and PB 013 (Gayasur pan). Based on incidence of foot and root rot on those cultivars, PB 001 (Laldingi) was graded as resistant, PB 011 (Jhal pan) and PB 013 (Gayasur pan) as moderately susceptible and rest of the cultivars as susceptible.
Bangladesh J. Agril. Res. 46(1): 63-69, March 2021
Hedonic Price Analysis for Hog Plum Purchase Decision In Southern Part of Bangladesh
Authors: Hossain A , Hoq MS , Mandal AK 
Published: January 2023

The hog plum (Spondias mombin) is locally known as Amra is very popular fruit in Bangladesh. It is very delicious fruit and sources of Vitamin C. The purpose of this study is to identify the importance of the attributes of hog plum on its price. A total of 121 respondents were interviewed to identify the attributes of hog plum purchase decision using both OLS and robust regression models. Results show that quantity, whole fresh and blemish free are important attributes for hog plum purchase decision. Quantity, blemish free and traceability are significantly important attributes for low income consumer groups whereas quantity and whole fresh are significantly important attributes for high income consumer groups. The implication of the study is that proper hog plum farming system and its marketing activities design are necessary for making it a profitable business.
Bangladesh J. Agril. Res. 46(1): 107-116, March 2021
Carvacrol and Cinnamaldehyde as Next Generation Antimicrobial Agents against Foodborne Pathogens: Antibacterial Efficacy and Synergistic Interaction with Nisin
Authors: Mishu Israt Dilruba , Akter Tanzia , Akhter Marufa Zerin , Hoque Md Mahfuzul 
Published: January 2023

Foodborne infections cause substantial issues all around the world, hence food safety is a major public health concern. The goal of this research is to provide information to the scientific community on the possible application of two plant essential oil molecules, Carvacrol (CAR) and Cinnamaldehyde (CN), as next-generation antimicrobials in the fight against foodborne pathogens. CAR and CN demonstrated outstanding dose-dependent antibacterial efficacy against 10 foodborne pathogens. Both Gram-positive and Gram-negative bacteria were suitably inhibited by the drugs. Antimicrobial activity of 10% v/v CAR and CN was much higher than that of the conventional antibiotic azithromycin (15g/disc). The study discovered that CAR and CN have very low MIC values (0.08% to 0.31% v/v), indicating that the compounds are efficacious even at very low concentrations. Even at 0.16% concentration, combining CAR and CN with Nisin (a widely available natural preservative) exhibited substantial synergism, with inhibition zones ranging from 8.5- 12.38 mm, decreasing the dose required to produce sufficient antimicrobial action. The findings imply that CAR and CN combined with nisin can be used as a natural antibacterial agent to limit the growth of foodborne pathogens and as a natural food preservative.
Bangladesh J Microbiol, Volume 39, Number 1, June 2022, pp 7-14
Class 1 Integron gene harbouring multidrug resistant Pseudomonas isolates from clinical specimen
Authors: Deuri Pranab , Bhowmik Anindita , Ahsan Sunjukta 
Published: January 2023

Gram-negative Pseudomonas spp. particularly P. aeruginosa is an important pathogen in hospitalized patients, contributing to the morbidity and mortality due to its multiple resistance mechanisms. The present study was undertaken to determine the characteristics of Pseudomonas spp. isolated from pus of patients admitted in a local diagnostic center, Dhaka, Bangladesh. A total of 14 clinical isolates were obtained. Detailed biochemical tests, antibiotic resistance pattern and class 1 integron gene profiling were successfully performed. All isolates (100%) showed resistance against amoxicillin-clavulanate, ampicillin, ciprofloxacin, cephalexin, ceftriaxone, kanamycin, nitrofurantoin and rifampicin. About 35% of the isolates (n=5) showed resistance against Amoxicillin-clavulanate, ampicillin, azithromycin, amikacin, ciprofloxacin, cephalexin, co-trimoxazole, ceftriaxone, imipenem, kanamycin, nitrofurantoin and rifampicin. Only 28.5% isolates (n=4) were sensitive to Imipenem. Plasmid was observed in none of the isolates, whereas 50% of the isolates carried Class 1 Integron gene. The presence of mobile genetic element is an added threat as this enables transfer of resistance genes to sensitive isolates. The findings of this study are significant from a public health viewpoint as therapeutic options become restricted.
Bangladesh J Microbiol, Volume 39, Number 1, June 2022, pp 15-19
Identification and Differentiation of Closely Related Members of Bacillus cereus Group by Multiplex PCR
Authors: Roy Sushmita , Saha Avirup , Khan Shafayet Imtiaze , Hasan Md Mahmud , Karim Muhammad Manjurul , Akhter Marufa Zerin , Hoq Md Mozammel , Khan Shakila Nargis 
Published: January 2023

Highly similar genetic and phenotypic traits of at least eight bacterial species forming the ‘Bacillus cereus group’ create their precise identification and differentiation quite difficult. The present study explores the applicability of a previously suggested multiplex-PCR method for the accurate identification of the candidate Bacillus species. Out of the 257 Bacillus isolates collected from soil, 44 were identified as B. thuringiensis and 39 as B. cereus by chromogenic cultural method using Bacillus agar, although few of them shared similar colony characteristics. Identification by the multiplex PCR in a thermo cycler using 5 different sets of primer-pairs, however produced distinct amplification corresponding to the bacterial species. Four of those pairs, named BMSH, BCJH, BTJH and BASH were designed based on gyrB gene that produced amplicons of four different sizes: 604, 475, 299 and 253 bp and were specific for B. mycoides, B. cereus, B. thuringiensis, and B. anthracis respectively. The remaining one of the sets was used as an internal control which was a universal primerpair, BCGSH, designed targeting a housekeeping gene, groEL that could produce an amplicon of 400 bp in polymerase chain reactions for all members of the B. cereus group, When probing the chromosomes extracted from 257 Bacillus isolates by multiplex PCR; 48, 39 and 5 were identified as B. thuringiensis, B. cereus, and B. anthracis respectively, however the rest of the isolates did not any amplification. Interestingly, the phylogenetic tree, constructed based on partial sequences of 16S rRNA genes of selected isolates including the reference strain of B. thuringiensis (HD-73, sotto) could not differentiate the species, instead posited those in a single cluster. The multiplex PCR, therefore, proved to be a sensitive and reliable method for the identification of the bacterial candidates of Bacillus cereus group than that of cultural and rRNA gene sequence analyses.
Bangladesh J Microbiol, Volume 39, Number 1, June 2022, pp 21-29
Metagenomic investigation of bacterial community of arsenic-prone area in the northwest region of Bangladesh
Authors: Rahman Khondaker Md Jaminur , Diba Farzana , Shuvo Md Sadikur Rahman , Siddique Mohammad Anwar , Hossain M Anwar , Sultana Munawar 
Published: January 2023

Metagenomic analysis provides in-depth understanding of microbe mediated Arsenic (As) metabolism. The present study aims atmetagenomic analysis of the distribution, diversity, and abundance of bacteriome in arsenic affected groundwater and surrounding soils collected fromBogra district of Bangladesh. Metagenomic DNA was extracted from two groundwater samples BCW3 andBCW4 (As content10μgL-1 and 500 μgL-1, respectively), and two tube-well surrounding soil samples BSS1 and BCS5 (As content335 μgkg-1 and 492 μgkg-1),where As rich water flows off. Metagenomic analysis of six hypervariable regions of 16S rRNA gene resulted in a total of 788709 processed sequence reads and 5878 operational taxonomic units (OTUs). Bacterial richness, abundance and diversity (alpha and beta) were higher in BCW4 (85 genera) than BCW3 (19 genera) whereas both soil samples exhibited almost similar richness and diversity. Predominant genera in BCW3 were Pseudomonas, Microbacterium, Achromobacterwhereas Acinetobacter, Thiothrix, Stenotrophomonas, Paracoccus, Dechloromonas dominated in BCW4. Soils were co-dominated with more than hundreds of genera with a high relative abundance of Bacillus, Rhodoplanesand Pseudomonas.Metagenomic investigation explored potentialarsenotrophicbacteriome. Exploring microbial community might help to understand the biogeochemistry of As affected groundwater and surrounding soil environment.
Bangladesh J Microbiol, Volume 39, Number 1, June 2022, pp 31-38
Behavioral, infrastructural and climate change Factors for Rising Diarrhea Episodes in Bangladesh
Authors: Bari Md Latiful , Emon Sharmin Zaman 
Published: January 2023

More than 4.5 million people nationwide have experienced diarrhea in the country’s most recent outbreak, which began on March, 2022. About 70% to 80% of patients (of whom 23% had severe diarrhea or cholera) were hospitalized. Several factors were associated with the risk for diarrhea include; unhygienic and unsafe environment, age, low educational status of mothers, employment status, family size, income, poor handling of drinking water at household level, place of residence, unhygienic disposal method of feces and wastes, lack of personal hygiene and lack of access to sanitation facilities. In addition, etiology, influence of climate and geographical location, diagnosis capability, hygiene education, and infrastructure are strongly linked to the high frequency of diarrhea and play a crucial role in efficiently managing diarrhea. We observed that a significant proportion of people with diarrheal illnesses continue to seek care from unqualified healthcare practitioners since inaccessibility from professional healthcare providers. Dissemination of information on health education, increasing the supply of skilled healthcare providers, and low-cost and quality healthcare services may encourage more people to seek care from professional healthcare providers, thus may help reducing mortality in the country. In conclusion, the policy mechanisms to increase systems’ resilience, some engineering approaches include source, treatment, distribution, and point-of-use control measures could be implemented to manage microorganism proliferation in drinking water.
Bangladesh J Microbiol, Volume 39, Number 1, June 2022, pp 39-45
The effects of Sodium-glucose cotransporter 2 inhibitors on adipose tissue in patients with type 2 diabetes: A meta-analysis of randomized controlled trials
Authors: Liu Xindong , Chen Ying , Liu Tao , Cai Ling , Yang Xiaofeng , Mou Chuan 
Published: January 2023

PurposeTo systematically evaluate the effect of Sodium-glucose cotransporter 2 (SGLT2) inhibitors on adipose tissue in patients with type 2 diabetes.MethodsWe searched PubMed, Cochrane Library, EMBASE, and Web of science databases for literature pertaining to Randomized controlled trials (RCTs) of SGLT2 inhibitors in treating type 2 diabetes patients. The retrieval time was from the date of establishment of the databases to September 1, 2022. Meta-analysis was performed using RevMan5.4 software.ResultsTotally 551 patients were included in 10 articles. Meta-analysis results showed that compared with the control group, the visceral adipose tissue (WMD = -16.29 cm2, 95% CI: -25.07 ~ -7.50, P&lt;0.00001), subcutaneous adipose tissue (WMD = -19.34 cm2, 95% CI: -36.27 ~ -2.41, P&lt;0.00001), body weight (WMD = -2.36 kg, 95% CI: -2.89 ~ -1.83, P&lt;0.00001) and triglyceride (WMD = -24.41 mg/dl, 95% CI: -45.79 ~ -3.03, P = 0.03) of the trial group significantly reduced.ConclusionSGLT2 inhibitors cause significant reductions in visceral adipose tissue, subcutaneous adipose tissue, body weight and triglycerides in type 2 diabetes patients, which may be attributed to the protective effect of the inhibitors on cardiovascular system.
Immunomodulatory effects of complex probiotics on the immuno-suppressed mice induced by cyclophosphamide
Authors: Ma Weiwei , Li Wenwen , Yu Shuang , Bian Hongsheng , Wang Yanyan , Jin Yang , Zhang Zhenhua , Ma Qing , Huang Lili 
Published: January 2023

IntroductionPrevious studies have reported the beneficial effects of Bifidobacterium animalis subsp. lactis XLTG11, Lacticaseibacillus casei Zhang, and Lactiplantibacillus plantarum P8, respectively. However, studies on the immunomodulatory enhancing effects of three complex probiotics have not been conducted. The aim of our study is to investigate the immunomodulatory effects of complex probiotics effect on the immunosuppressed mice induced by cyclophosphamide (CTX).MethodsAn immunocompromised mouse model was established by intraperitoneal injection of cyclophosphamide, which was gavage of different doses of complex probiotics and levamisole hydrochloride. The splenic and thymic indices, intestinal barrier, leukocyte and lymphocyte counts, percentage of splenic lymphocyte subpopulations, cytokine levels, and gut microbiota were determined.ResultsResults showed that the complex probiotics significantly elevated the spleen and thymus indices, increased the villi and crypt depth and the goblet cells. The leukocyte and lymphocyte counts and the percentage of splenic lymphocyte subpopulations in the CTX-treated mice were significantly elevated by the complex probiotics. In addition, the cytokines (IL-6, IL-10, IL-1β, and IFN-γ) were significantly increased after complex probiotic treatment. The complex probiotics restored the gut microbiota structure to the pattern of the control group by reducing the ratio of Firmicutes/Bacteroidetes and enhancing the relative abundances of specific microbiota that produced short-chain fatty acids.DiscussionThis study provides theoretical support for the immunity-enhancing function of the complex probiotics as well as a pharmacological basis for its further development and utilization.
Factors associated with COVID-19 preventive behaviors among taxi drivers in Bangkok
Authors: Deesua Amornrat , Kaewpan Wonpen , Kalampakorn Surintorn , Sillabutra Jutatip 
Published: January 2023

ObjectivesThis paper aimed to identify factors associated with COVID-19 preventive behaviors among taxi drivers in Bangkok.MethodsThis cross-sectional study included 401 taxi drivers. Data were analyzed using descriptive statistics. The association between predisposing factors, enabling factors, and reinforcing factors with COVID-19 preventive behaviors was analyzed by using analysis of variance and Pearson's Product Moment Correlation. Multiple linear regression analysis was used to determine the influencing factors in predicting COVID-19 preventive behaviors of taxi drivers.ResultsThe present findings revealed that income adequacy, support from family, co-workers, and healthcare professionals, perceived susceptibility, severity, benefits, barriers, and health motivation, accessibility to personal protective equipment for COVID-19 and preventative measures against COVID-19 from other agencies were associated with good COVID-19 preventive behaviors among taxi-driver in Bangkok during COVID-19 pandemic (R2 = 0.349, p = 0.008). The model could predict 34.9% of variance in COVID-19 preventative behavior among taxi drivers.ConclusionTaxi drivers should be encouraged to engage in appropriate preventive behaviors against COVID-19, emphasizing the individual and organizational levels. There should be a policy by organizations to promote the implementation of COVID-19 safety control standards to ensure safe working conditions. In addition, appropriate welfare benefits should be provided for taxi drivers, such as loans, personal protective equipment, and access to health services to improve COVID-19 preventive behaviors.
Study on the accessibility and affordability of 50 drugs in Wuhan based on the WHO/HAI standardization method
Authors: Dong Zuojun , Zhang Shaoya , Wu Shuaijun , Xie Xintong , Sun Guojun , Yu Xuanyao 
Published: January 2023

ObjectiveTo understand the availability and affordability of essential drugs in Wuhan since the implementation of the national essential medicine system, and to provide a basis for the subsequent formulation and improvement of related policies.MethodsUsing the standard survey method jointly developed by the WHO and Health Action International (HAI), a sample of 50 essential drugs was selected to investigate and evaluate their availability and affordability in public medical and health institutions and social retail pharmacies in Wuhan, using six diseases with high clinical morbidity as the targets.ResultsThe availability of the original drug and the lowest-priced generic drug in public hospitals is 26.4 and 42.47% respectively, and that in retail pharmacies is 26.8 and 54.4% respectively. The median price ratio of the original drug and the lowest-priced generic drug is 28.71 and 2.23 respectively in public hospitals, and 29.24 and 3.59 respectively in retail pharmacies; In addition to individual drugs, such as omeprazole, others are affordable. The availability of essential drugs in public hospitals in Wuhan is lower than that in social retail pharmacies, and the availability of the lowest-priced generic drugs is much higher than that of original drugs.ConclusionThe availability of essential drugs in public hospitals in Wuhan is lower than that in social retail pharmacies, and the availability of the lowest-priced generic drugs is much higher than that of original drugs. The price of the original drug is much higher than the international reference price; The price of medicines in public hospitals is lower than that in retail pharmacies;the overall condition of affordability is good, but there is a big gap between the affordability levels of original drugs and generic drugs, and the affordability of original drugs is relatively poor. It is recommended to adjust the relevant policies according to the actual situation of Wuhan city itself, moderately ensure the supply of original drugs, improve the price transparency of retail pharmacies, and ensure that the basic drug needs of the public are met.
Simultaneous Treatment of Complex Incisional Hernia and Stoma Reversal
Authors: Olona Carles , Sales Ricard , Caro-Tarragó Aleidis , Espina Beatriz , Casanova Raquel , Jorba Rosa 
Published: January 2023

Purpose: The simultaneous repair of incisional hernias (IH) and the reconstruction of the intestinal transit may pose a challenge for many surgeons. Collaboration between units specialized in abdominal wall and colorectal surgery can favor simultaneous treatment.Methods: Descriptive study of patients undergoing simultaneous surgery of complex IH repair and intestinal transit reconstruction from the start of treatment in a joint team. All interventions were performed electively and with the collaboration of surgeons experts in abdominal wall and colorectal surgery.Results: 23 patients are included. 11 end colostomies, 1 loop colostomy, 6 end ileostomies and 5 loop ileostomies. Seven (30%) patients presented with a medial laparotomy incisional hernia, 3 (13%) with a parastomal incisional hernia, and 13 (56%) with a medial and parastomal incisional hernia. Closure of the hernial defect was achieved in 100% of cases, and reconstruction of the intestinal tract was achieved in 22 (95%). Component separation was required in 17 patients (74%), which were 11 (48%) posterior and 6 (26%) anterior. In-hospital morbidity was 9%, and only two patients presented Clavien-Dindo morbidity &gt; III when requiring reoperation, one due to hemorrhage of the surgical bed and another due to dehiscence of the coloproctostomy. The mean follow-up was 11 months, with 20 (87%) patients having no complications. Mesh had to be removed in one patient with anastomotic dehiscence, no mesh had to be removed due to surgical site infection.