bluuebunny/crossref_metadata_2025_split huggingface dataset:
Investigating Neurometabolite Changes in Response to Median Nerve Stimulation
Authors: Houlgreave Mairi S. , Dyke Katherine , Berrington Adam , Jackson Stephen R. 
Published: January 2025

ABSTRACTBackgroundRhythmic median nerve stimulation (MNS) at 10 Hz has been shown to cause a substantial reduction in tic frequency in individuals with Tourette syndrome. The mechanism of action is currently unknown but is hypothesized to involve entrainment of oscillations within the sensorimotor cortex.ObjectiveWe used functional magnetic resonance spectroscopy (fMRS) to explore the dynamic effects of MNS on neurometabolite concentrations.MethodsHere, we investigated the effects of rhythmic and arrhythmic 10 Hz MNS on glutamate (Glu) and GABA concentrations in the contralateral sensorimotor cortex in 15 healthy controls, using a blocked fMRS design. We used a Mescher–Garwood‐semi‐localized by adiabatic selective refocusing (MEGA‐sLASER) sequence at 7 T.ResultsOur results show no difference in the difference‐from‐baseline measures between the two stimulation conditions. Looking at the effect of MNS over both conditions there is a trend for an initial increase in Glu/tCr (total creatine) followed by a decrease over time, whereas GABA/tCr decreased during each stimulation block.ConclusionsThese results suggest that despite entrainment of oscillations during rhythmic MNS, there are no significant differences in the tonic neuromodulatory effects of rhythmic and arrhythmic stimulation. The reduction in Glu over the course of stimulation may reflect a decrease in the glutamatergic firing due to adaptation. This may make it less likely that an involuntary movement is generated during continuous stimulation.
Barnahus Is Coming: A Reflexive Thematic Analysis of How Child Sexual Abuse Services at a Dublin Hospital Adapt and Respond
Authors: Lacey Eimear , Collins Mary 
Published: January 2025

ABSTRACTThe Barnahus Model, influencing how professionals across child protection, criminal justice and health come together to support families, has become the core model of response to concerns of child sexual abuse (CSA) in Europe and represents a significant social innovation currently being developed in Ireland. In 2019, Barnahus West in Galway, Ireland, was launched. Two additional services, Barnahus South and East, are in development. Nine senior professionals, across three internal services in a Dublin hospital and members of this organisations Barnahus steering group, participated in semistructured interviews on this topic. This reflexive thematic analysis, utilising the lenses of leadership, innovation and change, explores how already existing services based in this Dublin hospital may adapt and respond to facilitate this social innovation. Three themes were identified: Focus of Change Management, Barriers to Change and First Steps to Implementation. Recommendations include that a collective leadership approach is best suited to implementation of this change. The steering group would also benefit from utilising a formal change model to support their work. Small‐scale testing of an initial cross‐system response to CSA to inform wider implementation of systemic change is recommended.
Antimicrobial Potentials of Haliclona fibulata Derived Compounds for Combatting Drug‐Resistant Bacterial Infections in Zebrafish Model
Authors: Bajhal Sakshi , Seenivasan Boopathi , Arockiaraj Jesu , Sundaramurthy Anandhakumar 
Published: January 2025

ABSTRACTThe rising threat of antimicrobial resistance among pathogens highlights the critical need for novel antimicrobial agents. This study explores the potential of natural products by investigating n‐hexane extracts from the marine sponge Haliclona fibulata (HF) for their antibacterial efficacy. The well diffusion method of HF extract showed significant antibacterial activity against Pseudomonas aeruginosa, Escherichia coli, and Staphylococcus aureus with inhibition zones of 20 ± 0.66, 27 ± 0.58, and 22 ± 0.33 mm, respectively. Scanning electron microscopy investigations confirmed the disruptive impact of the extracts on bacterial cell morphology. Further investigations using P. aeruginosa‐infected zebrafish model revealed that HF treatment improved survival rates in a dose‐dependent manner. Additionally, oxidative stress and apoptosis were significantly reduced in the 100 µg/mL (HF100) treatment group, alongside downregulation of inflammatory markers such as il‐1β, nf‐kβ, tnf‐α, and inos. Histological assessments showed reduced intestinal damage and fewer circulating macrophages in HF100‐treated zebrafish larvae. Identifying potent bioactive compounds within the extracts suggests that HF could be a valuable source of natural antimicrobials, warranting further exploration for developing treatments against pathogenic infections.
Ce[N(SiMe3)2]3(THF)3‐Catalyzed Hydroboration of CO2, Esters and Epoxides with Pinacolborane: Selective Synthesis of Methanol in Multigram Scale
Authors: Hossain Md Jabed , Shah Brij Kumar , Dash Soumya Ranjan , Kashish  , Vanka Kumar , Khan Shabana 
Published: January 2025

AbstractIn this work, we have reduced CO2 with HBpin to afford borylated methanol product selectively in ~99 % yield using Ce[N(SiMe3)2]3(THF)3 as a catalyst. This led to multigram scale isolation of methanol obtained from CO2 reduction via the hydrolysis of borylated methanol, this establishes the potential of Ce[N(SiMe3)2]3(THF)3 as an efficient homogeneous catalyst for the bulk scale methanol synthesis. A practical application of this catalytic system was also shown by reducing CO2‐containing motorbike exhaust efficiently and selectively. Further, C−O bond activation of esters and epoxides using HBpin and 1–2 mol % of Ce[N(SiMe3)2]3(THF)3 at 60 °C afforded the borylated alcohols in good to excellent yields, which can easily be hydrolysed to the eco‐friendly corresponding alcohol. The stoichiometric experiments were performed to prove the formation of in‐situ generated cerium hydride [Ce]‐H as an active catalyst.
Harnessing the Sulfur‐for‐Oxygen Shift: A Magic Bullet for Dynamic Photophysical and Anticancer Activities of Indole‐Barbituric Acid Construct
Authors: Tyagi Kartikay , Kumari Reena , Venkatesh V. 
Published: January 2025

AbstractThe development of small molecule‐based drugs emerged as a cornerstone of modern drug discovery. Structural activity relationship (SAR) studies in medicinal chemistry are crucial for lead optimization, where a subtle change in the substituent can significantly alter its binding affinity with the biological target. Herein, a highly efficient single‐atom substitution (SAS) approach has been developed, where sulfur for oxygen strategy is utilized as a powerful molecular editing technique to identify N‐vinyl Indole‐thiobarbituric acid (6 a) as a novel small molecule‐based scaffold with tunable photophysical and antiproliferative activities. A series of NIR‐emitting indole‐barbituric/thiobarbituric acid conjugates exhibiting aggregation‐induced emission (AIE) were prepared, where the replacement of oxygen for sulfur strategy emerged as a magic bullet. On the evaluation of photophysical properties and chemopreventive efficacies, a significant improvement in the absorption and emission profile, cellular uptake, and antiproliferative activity was noted for sulfur counterparts. From the pool of the molecules, the lead molecule 6 a unveils a 55 nm emission shift, 142‐fold increased anticancer profile, and ~4‐fold elevated cellular uptake. Furthermore, the colocalization experiment unravels the nuclear localization of 6 a, where it causes severe DNA damage, arrests the cell cycle in the G2/M phase, and leads to the activation of p53‐mediated apoptosis. Our experimental findings represent 6 a as a potential lead molecule possessing excellent anticancer potency in the HCT 116 cell line and HCT 116‐derived 3D spheroid model.
Mitigating Climate Policy Shocks Through Mergers and Acquisitions? An Empirical Investigation of International Oil and Gas Firms
Authors: Chen Yajie , Zhou Chengchen , Zhang Dayong , Xie Jun , Managi Shunsuke 
Published: January 2025

ABSTRACTClimate change and its related policies have significant impacts on energy industries, leading to a considerable number of stranded assets and poor financial performance. Using a global sample of 1147 listed oil and gas firms from 2000 to 2021, this paper investigates whether mergers and acquisitions (M&As) mitigate climate policy shocks, focusing on the consequential financial impacts. Taking the Paris Agreement as the major climate policy shock, we first confirm the negative impacts of climate policy on the financial performance of oil and gas firms, after which we demonstrate M&As can alleviate the adverse effects. Mechanism analysis reveals that the financial benefits of M&As are stronger in upstream firms, those with better corporate governance and sufficient cash flows. Firms in countries with high‐level economic development and carbon risk can benefit from M&As. Furthermore, conglomerate M&As increased following the Paris Agreement, indicating that these energy firms responded to climate policy shocks through diversification. These findings can help us understand the global impacts of climate policies and have important implications for how the energy sector should respond to policy shocks.
Nanopore adaptive sampling accurately detects nucleotide variants and improves the characterization of large‐scale rearrangement for the diagnosis of cancer predisposition
Authors: Chevrier Sandy , Richard Corentin , Mille Marie , Bertrand Denis , Boidot Romain 
Published: January 2025

AbstractBackgroundMolecular diagnosis has become highly significant for patient management in oncology.MethodsHere, 30 well‐characterized clinical germline samples were studied with adaptive sampling to enrich the full sequence of 152 cancer predisposition genes. Sequencing was performed on Oxford Nanopore (ONT) R10.4.1 MinION flowcells with the Q20+ chemistry.ResultsIn our cohort, 11 samples had large‐scale rearrangements (LSR), which were all detected with ONT sequencing. In addition to perfectly detecting the locus of the LSR, we found a known MLPA amplification of exon 13 in the BRCA1 (NM_7294) gene corresponded to a duplication in tandem of both exons 12 and 13 of the reference NM_7300. Similarly, in another sample with a known total deletion of the BRCA1 gene, ONT sequencing highlighted this complete deletion was the consequence of a large deletion of almost 140 000 bp carrying over five different genes. ONT sequencing was also able to detect all pathogenic nucleotide variants present in 16 samples at low coverage. As we analyzed complete genes and more genes than with short‐read sequencing, we detected novel unknown variants. We randomly selected six new variants with a coverage larger than 10× and an average quality higher than 14, and confirmed all of them by Sanger sequencing, suggesting that variants detected with ONT (coverage >10× and quality score >14) could be considered as real variants.ConclusionsWe showed that ONT adaptive sampling sequencing is suitable for the analysis of germline alterations, improves characterization of LSR, and detects single nucleotide variations even at low coverage.Key points
Adaptive sampling is suitable for the analysis of germline alterations.
Improves the characterization of Large Scale Rearrangement and detects SNV at a minimum coverage of 10x.
Allows flexibility of sequencing.
Gene Expression After Exercise Is Disrupted by Early‐Life Stress
Authors: Campbell Taylor S. , Donoghue Katelyn , Roth Tania L. 
Published: January 2025

ABSTRACTExercise can be leveraged as an important tool to improve neural and psychological health, either on its own or to bolster the efficacy of evidence‐based treatment modalities. Research in both humans and animal models shows that positive experiences, such as exercise, promote neuroprotection while, in contrast, aversive experiences, particularly those in early development, are often neurologically and psychologically disruptive. In the current study, we employed a preclinical model to investigate the therapeutic benefits of exercise on gene expression in the brains of adult rats. Long Evans rats were exposed to maltreatment stress or nurturing care during infancy, with some rats later given voluntary running wheels as an aerobic exercise intervention from Postnatal Days 70 to 90. Our results showed that irisin gene expression, which promotes neuroprotection, was differentially affected by exercise and early exposure to stress. We add to a rapidly growing area of research on the neuroprotective benefits of exercise and shed light on important molecular mechanisms that may affect the efficacy of exercise in different individuals.
Carbon Storage Response to Land Use/Land Cover Changes and SSP‐RCP Scenarios Simulation: A Case Study in Yunnan Province, China
Authors: Liu Jing , Yang Kun , Zhang Shaohua , Zeng Wenxia , Yang Xiaofang , Rao Yan , Ma Yan , Bi Changyou 
Published: January 2025

ABSTRACTChanges in terrestrial ecosystem carbon storage (CS) affect the global carbon cycle, thereby influencing global climate change. Land use/land cover (LULC) shifts are key drivers of CS changes, making it crucial to predict their impact on CS for low‐carbon development. Most studies model future LULC by adjusting change proportions, leading to overly subjective simulations. We integrated the Integrated Valuation of Ecosystem Services and Trade‐offs (InVEST) model, the Patch‐generating Land Use Simulation (PLUS) model, and the Land Use Harmonization 2 (LUH2) dataset to simulate future LULC in Yunnan under different SSP‐RCP scenarios of climate and economic development. Within the new PLUS‐InVEST‐LUH2 framework, we systematically analyzed LULC alterations and their effects on CS from 1980 to 2040. Results demonstrated that: (1) Forestland had the highest CS, whereas built‐up land and water showed minimal levels. Western areas boast higher CS, while the east has lower. From 1980 to 2020, CS continuously decreased by 29.55 Tg. In the wake of population increase and economic advancement, the area of built‐up land expanded by 2.75 times. Built‐up land encroaches on other land categories and is a key cause of the reduction in CS. (2) From 2020 to 2040, mainly due to an increase in forestland, CS rose to 3934.65 Tg under the SSP1‐2.6 scenario, whereas under the SSP2‐4.5 scenario, primarily due to a reduction in forestland and grassland areas, CS declined to 3800.86 Tg. (3) Forestland is the primary contributor to CS, whereas the ongoing enlargement of built‐up land is causing a sustained decline in CS. Scenario simulations indicate that future LULC changes under different scenarios will have a significant impact on CS in Yunnan. Under a green sustainable development pathway, Yunnan can exhibit significant carbon sink potential. Overall, this research offers a scientific reference for optimizing land management and sustainable development in Yunnan, aiding China's “double carbon” goals.
Ruthenium‐Zeolite Catalyzed Dehydrative N‐Benzylation of Amides for the Construction of Macrolactams Under Continuous Flow
Authors: Bandu Jamdade Akash , Bamayya Swami Gourishankar , Vishambar Sutar Dashrat , Nair Padmavathy V. K. , Gnanaprakasam Boopathy 
Published: January 2025

AbstractContinuous‐flow synthesis of acyclic amides and macrolactams has been demonstrated successfully via N‐benzylation of amides using a highly reusable heterogeneous Ru‐Zeolite as a catalyst, which gives only water as the byproduct. N‐Benzylation of acyclic amides furnished 30 N‐benzylated amides whereas intramolecular N‐benzylation with alcohols was well tolerated with 15 examples of macrolactams bearing 11‐ to 15‐membered rings. Notably, the scalability of this flow process has been demonstrated from milligrams to gram scale using the Ru‐Zeolite heterogeneous catalyst. Moreover, the reusability of the catalyst is exemplified by the 16 reaction cycles carried out with one time‐packed heterogeneous catalyst in the reactor bed.
Followers’ Locomotion Mode Predicts Preferring Assertive Leaders: Regulatory Fit by Means of Valued Social Characteristics of Others
Authors: Beylat Magali , Woltin Karl‐Andrew , Barbedor Julien , Sassenberg Kai , Yzerbyt Vincent 
Published: January 2025

ABSTRACTBuilding on prior work on regulatory fit in leader–follower contexts, the present work investigated such fit regarding followers’ locomotion mode (the concern to move forward and maintain action flow) and leader assertiveness. In three studies, we recruited English‐speaking employees online (Ntotal = 948) and assessed their regulatory mode. In Study 1, we then measured participants’ preferences regarding characteristics their leaders ideally should have. In Study 2, participants reported perceived characteristics (ability/assertiveness/morality/friendliness) of their actual leader. In Study 3, we presented participants with a highly assertive versus highly able leader profile. Participants subsequently indicated their expected satisfaction with and motivation by these leaders. Across studies, the higher the followers’ locomotion mode, the more they valued their leader being assertive. These findings show that feelings of fit can also derive from an alignment with others’ characteristics and shed light on when leader assertiveness is appreciated.
Optimizations of Placenta Extracellular Matrix‐Loaded Silk Fibroin/Alginate 3D‐Printed Scaffolds Structurally and Functionally for Bone Tissue Engineering
Authors: Bashiri Zahra , Khosrowpour Zahra , Moghaddaszadeh Ali , Jafari Davod , Alizadeh Sanaz , Nasiri Hajar , Parsaei Houman , Keshtkaran Zahra , Abdollahpour‐Alitappeh Meghdad , Bargrizaneh Farshad , Rezaei Behzad , Simorgh Sara , Gholipourmalekabadi Mazaher 
Published: January 2025

ABSTRACTRecent interest has been focused on extracellular matrix (ECM)–based scaffolds totreat critical‐sized bone injuries. In this study, urea was used to decellularize and solubilize human placenta tissue. Then, different concentrations of ECM were composited with 8% alginate (Alg) and 12% silk fibroin (SF) for printing in order to produce a natural 3D construct that resembled bone tissue. The physical and biological features of the printed structures were evaluated entirely in vitro. Finally, a rat model was employed to examine the optimal 3D printed scaffold (5% ECM) as a bone transplant for the healing of cranial bone lesions. The present investigation demonstrated that decellularizing placental tissue fragments led to efficient removal of cell debris. In addition, a remarkable improvement in the printed scaffolds' mechanical and biological properties was observed by increasing the ECM concentration. The histology studies and real‐time PCR results demonstrated the acceleration of bone regeneration in the bone lesions treated with 5%ECM‐SF/Alg at 4 and 8 weeks after implantation. Overall, these results proved that the placental ECM‐printed scaffolds could potentially construct biomimetic grafts to reconstruct significant bone defects and now promise to proceed with clinical studies.
Fault Diagnosis Model via Vibration Signal Analysis With an Improved BKA‐VMD and CNN‐TELM Hybrid Framework
Authors: Yang Jingzong , Li Xuefeng , Mao Min 
Published: January 2025

ABSTRACTRolling bearings are fundamental components of contemporary machinery, yet their prolonged usage frequently leads to wear, performance deterioration, and potential faults. In scenarios characterized by limited sample sizes and complex, noisy environments, traditional diagnostic methods often encounter difficulties achieving satisfactory fault identification results. To address these challenges, this study introduces an innovative approach for rolling bearing fault diagnosis. Initially, the black‐winged kite algorithm (BKA) is enhanced through the integration of a differential evolution strategy and an iterative search method, enabling the precise determination of optimal parameters for variational mode decomposition (VMD). Subsequently, a comprehensive index evaluation criterion is established to identify the optimal signal components, which are then subjected to a detailed analysis to extract diverse sensitive features, ultimately forming a hybrid feature set. To further improve the accuracy and efficiency of fault diagnosis, this study proposes an enhanced extreme learning machine model, termed twin extreme learning machine (TELM). Moreover, the TELM model is seamlessly integrated into the architecture of a convolutional neural network (CNN), specifically as a component of its output layer, resulting in a novel hybrid fault diagnosis model. Rigorous data validation performed on a rolling bearing testbed underscores that the proposed fault diagnosis model significantly surpasses conventional approaches, including SVM, KELM, ELM, LSTM, and softmax, in terms of accuracy, recall, and F1 score. Notably, the model maintains robust fault diagnosis capabilities even in environments with varying degrees of noise interference.
Seasonal Analysis of Pathogenic Escherichia coli Contamination in Vegetables, Washing Water, and Vendor Hygiene: Virulence Group Classification and Antibiotic Resistance
Authors: Ahmad Zahra Maftuha , Niaj Murshidi Golam , Das Moon Unmilita , Sultana Sumaiya , Haque Fahim Kabir Monjurul 
Published: January 2025

ABSTRACTThis study, conducted between June 2022 and March 2023 in Dhaka, examined Escherichia coli prevalence in 874 samples from vegetables, vegetable wash water, and hand swabs from vendors during summer and winter. Of the total samples, 782 (89.50%) tested positive for E. coli , with 95.52% of samples in summer and 80.87% in winter. While overall E. coli prevalence showed no significant seasonal difference, pathotype prevalence was significantly higher in summer across all sample types, except for the CVD432 gene. E. coli isolated from spring onions had the highest prevalence of E. coli O157:H7 (19.23%) and the stx1 gene (30.76%), while capsicum isolates showed the highest prevalence of stx2 (40.00%), eaeA (20.00%), ipaH (35.00%), and eltB (20.00%) genes. In winter, coriander had the highest E. coli O157:H7 (14.28%), and cucumber isolates had the highest stx1 (19.04%) gene. Isolates from tomato and capsicum recorded elevated stx2 levels (16.00%). Carrot isolates exhibited the highest eaeA prevalence (11.42%), coriander isolates had the highest ipaH occurrence (14.28%), and tomato isolates had the highest eltB levels (16.00%). A significant seasonal difference was observed in only the stx1 gene, which was higher in summer for all vegetables. Antibiotic susceptibility testing of 1206 isolates revealed widespread resistance, particularly to ampicillin and erythromycin. Significant seasonal differences in resistance were noted in vegetable samples, but not in water and hand swab samples. Multidrug resistance was highest in isolates from spring onions (56.60% in summer) and carrots (71.87% in winter), with extensively drug‐resistant isolates highest in mint (2.17% in summer) and carrots (6.25% in winter).