bluuebunny/crossref_metadata_2025_split huggingface dataset:
Covalent Targeting of Glutamate Cysteine Ligase to Inhibit Glutathione Synthesis**
Authors: Zhang Lydia H. , Tang Michelle , Tao Xavier , Shao Qian , Thomas Vienna , Shimizu Saki , Kasano Miki , Ishikawa Yoshinori , Inukai Takayuki , Nomura Daniel K. 
Published: September 2023

AbstractDysregulated oxidative stress plays a major role in cancer pathogenesis and some types of cancer cells are particularly vulnerable to inhibition of their cellular antioxidant capacity. Glutamate‐cysteine ligase (GCL) is the first and rate‐limiting step in the synthesis of the major cellular antioxidant glutathione (GSH). Developing a GCL inhibitor may be an attractive therapeutic strategy for certain cancer types that are particularly sensitive to oxidative stress. In this study, we reveal a cysteine‐reactive ligand, EN25, that covalently targets an allosteric cysteine C114 on GCLM, the modifier subunit of GCL, and leads to inhibition of GCL activity. This interaction also leads to reduced cellular GSH levels and impaired cell viability in ARID1A‐deficient cancer cells, which are particularly vulnerable to glutathione depletion, but not in ARID1A‐positive cancer cells. Our studies uncover a novel potential ligandable site within GCLM that can be targeted to inhibit GSH synthesis in vulnerable cancer cell types.
LPMO‐like Activity of Bioinspired Copper Complexes: From Model Substrate to Extended Polysaccharides
Authors: Leblay Rébecca , Delgadillo‐Ruíz Rafael , Decroos Christophe , Hureau Christelle , Réglier Marius , Castillo Ivan , Faure Bruno , Simaan A. Jalila 
Published: September 2023

AbstractPolysaccharide oxidative depolymerization is highly desirable to achieve recalcitrant biomass valorization. Inspired by recently discovered Lytic Polysaccharide Monooxygenases, mononuclear copper complexes have been prepared and studied in the literature. However, the activities were evaluated on different substrates and under various conditions. In this work we intended to establish a robust and reproducible activity assay, in aqueous solution at a pH close from neutrality and under mild conditions. We have evaluated several complexes on substrates of increasing complexity: the model substrate para‐nitrophenyl‐β‐D‐glucopyranoside (p‐NPG), cellobiose (glucose dimer), as well as on extended substrates (chitin, cellulose and bagasse from agave). The different assays were compared and proof‐of‐concept that bioinspired complexes can oxidatively promote polysaccharide depolymerization was obtained. Finally, we measured level of hydroxyl radicals released by the complexes under comparable experimental conditions and mechanistic pathways are discussed.
Fluorogen‐Functionalized Mesoporous Silica Hybrid Sensing Materials: Applications in Cu2+ Detection
Authors: Xu Lijie , Jiang Xiaoping , Liu Yuhong , Liang Kang , Gao Meng , Kong Biao 
Published: September 2023

AbstractSince Cu2+ ions play a pivotal role in both ecosystems and human health, the development of a rapid and sensitive method for Cu2+ detection holds significant importance. Fluorescent mesoporous silica materials (FMSMs) have garnered considerable attention in the realm of chemical sensing, biosensing, and bioimaging due to their distinctive structure and easily functionalized surfaces. As a result, numerous Cu2+ sensors based on FMSMs have been devised and extensively applied in environmental and biological Cu2+ detection over the past few decades. This review centers on the recent advancements in the methodologies for preparing FMSMs, the mechanisms underlying sensing, and the applications of FMSMs‐based sensors for Cu2+ detection. Lastly, we present and elucidate pertinent perspectives concerning FMSMs‐based Cu2+ sensors.
Good Practices and Practical Considerations for Research with Perfluoroalkyl Substances
Authors: Taylor Chloe M. , Breadmore Michael C. , Kilah Nathan L. 
Published: September 2023

AbstractPer‐ and polyfluoroalkyl substances (PFAS) are a class of hazardous pollutant that are ubiquitous in our modern world. Current research is driven by an increased awareness and concern regarding the safety of PFAS for the general population, while tightening regulations have prompted the need for detection and quantification techniques from a wide range of matrices. PFAS are a group of molecules offering unique behaviours, hazards, and complications that are not obvious or readily apparent from reading the literature. These peculiarities can impede or convolute analyses when not considered in experimental design. Drawing on the knowledge of a range of literature sources, this tutorial review looks to highlight and amalgamate the valuable suggestions and methodologies currently available to enable successful PFAS research in any laboratory.
Acyclic Cucurbit[n]urils: Effective Taste Masking Nanocontainers for Cationic Bitter Compounds
Authors: Peng Wen‐Chang , Lei Zhuo , Lin Qi‐Han , Wu Yan , Yang Jing‐Yu , Wang Hui , Zhou Wei , Zhang Dan‐Wei , Li Zhan‐Ting , Ma Da 
Published: September 2023

AbstractNew acyclic cucurbit[n]urils (ACBs) with eight carboxylate groups were synthesized. These hosts are highly soluble in water, and can form stable inclusion complexes with cationic bitter compounds. ACBs are confirmed to be non‐toxic and biocompatible. Two‐bottle preference (TBP) tests on mice show that all ACBs are tasteless to mammals. ACBs are discovered to mask the bitterness of berberine and denatonium benzoate, but not quinine hydrochloride, due to different binding modes.
Higher‐Order Electrocyclizations in Biological and Synthetic Processes
Authors: de Cózar Abel , Arrieta Ana , Arrastia Iosune , Cossío Fernando P. 
Published: September 2023

AbstractIn general, electrocyclizations follow the Woodward‐Hoffmann's rules of conservation of orbital symmetry. These rules have been extensively verified in low‐order processes, both in thermal and photochemical reactions, up to eight π‐electrons. However, when the number of π‐electrons in the system increases, some deviations of that general rules can be found. This focused review highlights the main features of reported higher‐order electrocyclizations involving 10, 12, 14, 16 and 18 π‐electrons. Some of these examples constitute useful intermediates in the synthesis of biologically active compounds. When computational studies were not included in the reported examples, DFT calculations have been performed to be included in this review. Analysis of the respective pericyclic topologies shows the importance of computational tools for understanding the selectivity observed experimentally.
Calling Cards: A Customizable Platform to Longitudinally Record Protein‐DNA Interactions Over Time in Cells and Tissues
Authors: Yen Allen , Mateusiak Chase , Sarafinovska Simona , Gachechiladze Mariam A. , Guo Juanru , Chen Xuhua , Moudgil Arnav , Cammack Alexander J. , Hoisington‐Lopez Jessica , Crosby MariaLynn , Brent Michael R. , Mitra Robi D. , Dougherty Joseph D. 
Published: September 2023

AbstractCalling Cards is a platform technology to record a cumulative history of transient protein‐DNA interactions in the genome of genetically targeted cell types. The record of these interactions is recovered by next‐generation sequencing. Compared with other genomic assays, readouts of which provide a snapshot at the time of harvest, Calling Cards enables correlation of historical molecular states to eventual outcomes or phenotypes. To achieve this, Calling Cards uses the piggyBac transposase to insert self‐reporting transposon “Calling Cards” into the genome, leaving permanent marks at interaction sites. Calling Cards can be deployed in a variety of in vitro and in vivo biological systems to study gene regulatory networks involved in development, aging, and disease. Out of the box, it assesses enhancer usage but can be adapted to profile‐specific transcription factor (TF) binding with custom TF‐piggyBac fusion proteins. The Calling Cards workflow has five main stages: delivery of Calling Cards reagents, sample preparation, library preparation, sequencing, and data analysis. Here, we first present a comprehensive guide for experimental design, reagent selection, and optional customization of the platform to study additional TFs. Then, we provide an updated protocol for the five steps, using reagents that improve throughput and decrease costs, including an overview of a newly deployed computational pipeline. This protocol is designed for users with basic molecular biology experience to process samples into sequencing libraries in 2 days. Familiarity with bioinformatic analysis and command line tools is required to set up the pipeline in a high‐performance computing environment and to conduct downstream analyses. © 2023 Wiley Periodicals LLC.This article was corrected on 16 October 2023. See the end of the full text for details.Basic Protocol 1: Preparation and delivery of Calling Cards reagentsSupport Protocol 1: Next‐generation sequencing quantification of barcode distribution within self‐reporting transposon plasmid pool and adeno‐associated virus genomeBasic Protocol 2: Sample collection and RNA purificationSupport Protocol 2: Library density quantitative PCRBasic Protocol 3: Sequencing library preparationBasic Protocol 4: Library pooling and sequencingBasic Protocol 5: Data analysis
Fabrication Technique of Micropatterned Inverse Photonic Crystal Films
Authors: Ashurov Matin , Liu Yuchen , Ezhov Alexander , Din Salah Ud , Baranchikov Alexander , Klimonsky Sergey 
Published: September 2023

AbstractA simple and frugal method for fabricating inverse micropatterned photonic crystal (PhC) films aimed at miniaturization and multiplexing of opaline PhCs is described. First, highly uniform micropatterned synthetic opal structures in the form of periodic stripes are formed via stick‐slip motion of the meniscus during SiO2 colloid solution evaporation. The prepared opal structures are then used as templates to produce inverse stripe patterned films with highly uniform colors via photopolymerization of ethoxylate trimethylolpropane triacrylate (ETPTA) photocurable resin. Local reflectance spectra are recorded to prove the PhC properties of the inverse stripes, and peaks over 40% associated with the first photonic stop band are observed. The readily fabrication process suggested here is an important step toward the development of many applications that can pave the technical road map for the next wave of innovations and breakthrough in PhCs for sensing. In particular, the resulting structures can be used as PhC arrays containing a line of periodically repeating sensor strips capable to detect the content of alcohols in water.
Determination of chlorphenesin in cosmetics, a preservative that can affect anti‐doping test result interpretations
Authors: Lu Yi‐Wen , Chan Kuei‐Hui , Wu Yu‐Tse , Chang William Chih‐Wei 
Published: September 2023

AbstractChlorphenesin is a legitimate preservative commonly used in cosmetics. It shares one urinary metabolite of 4‐chlorophenoxyacetic acid with meclofenoxate, a prohibited stimulant in sports. Recently, there have been cases where athletes using chlorphenesin‐containing products were falsely identified as users of meclofenoxate. This study developed and validated a liquid chromatography method with diode‐array detection to determine the chlorphenesin content in 61 selected personal care products with various functions (e.g., facial care, body cleansing, sun protection, make‐up, hairstyling, perfume, and oral cleaning). The analytical method demonstrated fit‐for‐purpose quantitation and provided good linearity, precision, accuracy, and recovery for analyzing different cosmetic matrices. Among the 27 cosmetics labeled with chlorphenesin, the chlorphenesin concentrations ranged from 0.10 to 2.67 mg/g, with three products showing no detection. None of the products exceeded the maximum limit of 3 mg/g (0.3%) set by regulatory authorities. Among the 34 cosmetics not labeled with chlorphenesin, none of them contained chlorphenesin. This study confirmed the absence of undeclared chlorphenesin in the selected cosmetics, supporting the correctness of chlorphenesin labeling in cosmetics sold in Taiwan. Further investigations studying urinary excretion patterns after different types, doses, frequencies, and sites of cosmetics applications could contribute to strengthen current testing approaches in anti‐doping.
Fundamental experiment and numerical simulation of pre‐ionized inert gas plasma MHD electrical power generation
Authors: Kimsor Ork , Kodera Yoshinobu , Okuno Yoshihiro 
Published: September 2023

AbstractThe experiments of pre‐ionized inert gas plasma MHD electrical power generation are conducted, and the performance and plasma behavior in the experimental generator are examined through time‐dependent r‐_ two‐dimensional numerical simulation. In the experiment, an enthalpy extraction ratio of 4.01% has been obtained with a disk‐shaped MHD generator with radio‐frequency pre‐ionization. In the numerical simulation, at an assumed inlet electron temperature around 5600 K (inlet ionization degree 0:10 × 10−4)_6600 K (1:36 × 10−4), the plasma structure is similar to the non‐uniform structure observed in the experiment. An enthalpy extraction ratio around 2_5% matches well with that in the experiment. At a suitable inlet electron temperature of 7000 K (3:15 × 10−4)_8000 K (1:79 × 10−3), although non‐uniform plasma structure still occurs, a high enthalpy extraction ratio over 10% is expected.
A Hydrophobic Deep Eutectic Solvent for Nuclear Fuel Cycle: Extraction of Actinides and Dissolution of Uranium Oxide
Authors: Gamare Jayashree , Vats Bal Govind 
Published: September 2023

AbstractHydrophobic Deep Eutectic Solvents (DESs) have been attracting attention for metal ion extraction in solvent extraction process due to their favorable properties. A Trioctyl phosphine oxide (TOPO) and Thenoyl trifluoroacetone (HTTA) based hydrophobic DES was synthesized and characterized by FTIR and NMR spectroscopy. Actinide ions ( UO22+, Pu4+ and Am3+) and Eu3+ ion extraction was carried out using the DES which shows that it extracts these metal ions from aqueous nitric acid medium depending upon the molarity of nitric acid. At higher molarity of nitric acid (>5 M) the extraction becomes insignificant only for trivalent metal ions and open up the possibility to selectively strip trivalent metal ions from tetravalent and hexavalent ions. This DES was also used for dissolution of uranium oxide (UO3). The dissolution kinetics was studied and it was shown that oxide was dissolved within an hour at 80 °C. The maximum solubility of UO3 in DES was measured and found to be 130±5 mg/mL which is one of the highest reported solubility of UO3 in ILs and DES. The species of uranium which is formed in situ in DES was ascertained to be UO2(TTA)2.TOPO after dissolution of UO3 as supported by FTIR and NMR (1H and 31P) investigations.
Cellular Mechanistic Considerations on Cytotoxic Mode of Action of Phosphino Ru(II) and Ir(III) Complexes
Authors: Wojtala Daria B. , Komarnicka Urszula K. , Kyzioł Agnieszka , Kozieł Sandra , Szmitka Magdalena , Słowikowski Mateusz , Kulczyńska Julia , Stochel Grażyna 
Published: September 2023

AbstractTwo piano‐stool ruthenium(II) complexes Ru(η6‐p‐cymene)Cl2PPh2CH2OH (RuPOH) and Ru(η6‐p‐cymene)Cl2P(p‐OCH3Ph)2CH2OH (RuMPOH) and two half‐sandwich iridium(III) complexes Ir(η5‐Cp*)Cl2PPh2CH2OH (IrPOH) and Ir(η5‐Cp*)Cl2P(p‐OCH3Ph)2CH2OH (IrMPOH) have been studied in terms of potential anticancer activity on previously selected cell line (human lung adenocarcinoma). Based on experimental results obtained in monoculture in vitro model mechanistic considerations on the possible cellular modes of action have been carried out. ICP‐MS analysis revealed the higher cellular uptake for less hydrophobic Ir(III) complexes in comparison to the corresponding Ru(II) compounds. Cytometric analysis showed a predominance of apoptosis over the other types of cell death for all complexes. The apoptotic pathway was confirmed by a decrease in mitochondrial membrane potential and the activation of caspases‐3/9 for both Ru(II) and Ir(III) complexes. It was concluded that in the case of Ru(II) complexes the intense ROS generation is mainly responsible for the resulting cytotoxicity. The corresponding Ir(III) complexes trigger simultaneously at least three different cytotoxic pathways i. e., depletion of mitochondrial potential, activation of caspases‐dependent apoptosis, and ROS‐associated oxidation. Thus, it can be assumed that the final accumulation of toxic effects over time via parallel activation of different pathways results in the highest cytotoxicity in vitro exhibited by Ir(III) complexes when compared with Ru(II) complexes.
Market research of infant formula milks in Spain
Authors: Albertos Irene , Romero Janira , Piqueras‐Picón Alicia , García Sara , López María , Jiménez José María , Alija María José Castro 
Published: September 2023

AbstractThere are circumstances in which breastfeeding is not possible and formula milk feeding has to be resorted to. In these cases, it is difficult to choose among the multitude of brands on the market for infant feeding. Although the composition of formula milk is largely regulated by legislation, there are certain nutrients whose presence in breast milk is beneficial. This circumstance will help us to establish some criteria to choose formula milk for a healthy infant. Among all the formula milks which can be found in different shops (pharmacies, supermarkets, etc.), market research has been carried out based on the nutritional criteria: the lipid and protein profile, certain carbohydrates, amino acids, and vitamins, as well as the presence of nucleotides, prebiotics, and probiotics. Based on these results, it can be established which formula milk we would give to a healthy infant. Thus, within the analyzed formula milks, it has been concluded that the most complete milk in this would be formula milk D and the least formula milk J. Although more studies are needed to confirm this, it is foreseeable that, as the scientific evidence is greater, the legislation will be updated and considering these nutrients for a correct formula milk.
Can technical indicators based on underlying assets help to predict implied volatility index
Authors: Yafeng Shi , Shi Yanlong , Tingting Ying 
Published: September 2023

AbstractGiven the widespread use of technical analysis and the tight relationship between derivatives and the underlying assets, we employ the copula approach to investigate whether the technical indicators based on underlying assets convey extra information about the future movements of implied volatility (IV) indexes. The empirical results, based on long samples of five well‐known IV indexes, suggest that although the technical indicators are not informative for forecasting the future prices of IV indexes, they can provide extra information about the size of forecasting errors of the IV indexes. The findings are also robust to the impact of COVID‐19. The technical indicators are then used to extend Threshold ARCH and Exponencial GARCH models for improving the estimation of Value at Risks (VaRs). The out‐of‐sample forecast results show that the proposed model outperforms the benchmark in estimating the VaRs. These findings have implications for pricing options of IV indexes and managing the risks of IV‐related portfolios.
Rapid Synthesis of Propargyl‐γ‐Modified Peptide Nucleic Acid Monomers for Late‐Stage Functionalization of Oligomers
Authors: López‐Tena Miguel , Watson Emma E. , Romanens Patrick , Winssinger Nicolas 
Published: September 2023

AbstractThe exceptional hybridization properties of peptide nucleic acids (PNAs) coupled with the ease of their synthesis has made this artificial nucleic acid mimetic a desirable platform for diagnostics, therapeutics and supramolecular engineering. PNA backbone modifications have been extensively explored to finetune physicochemical properties and for conjugation of functional molecules. Here, we detail the synthesis of a universal γ‐propargyl‐PNA backbone from serine, and its acylation with the four DNA canonical nucleobases. The availability of serine as d or l enantiomer provide simple accesses to PNA oligomers for hybridization with natural oligonucleotides or for orthogonal hybridization circuitry. We show that late‐stage conjugation enables optimization of the physicochemical properties. This approach is appealing due to its orthogonality to Fmoc‐SPPS, its flexibility and ease for introducing diversity by on‐resin copper(I)‐catalyzed azide‐alkyne cycloaddition (CuAAC). We exemplified the utility of these novel monomers with PNA based hybridization chain reactions (HCRs).
Comparison of autologous, matched sibling, and alternative donor stem cell transplant outcomes for acute myeloid leukemia patients in first remission: A propensity score matching study
Authors: Wang Mingyang , Zhang Haixiao , Zheng Xinhui , Liu Jia , Wang Jiali , Cao Yigeng , Zhang Xiaoyu , Zhang Rongli , Chen Xin , Zhai Weihua , Ma Qiaoling , Wei Jialin , Huang Yong , Yang Donglin , He Yi , Pang Aiming , Feng Sizhou , Han Mingzhe , Jiang Erlie 
Published: September 2023

AbstractAutologous hematopoietic stem cell transplantation (auto‐HSCT), matched sibling donor HSCT (MSD‐HSCT), and alternative donor HSCT (AD‐HSCT) are viable post‐remission treatment options for acute myeloid leukemia (AML). A total of 283 de novo favorable‐ and intermediate‐risk AML patients, based on the ELN 2022 criteria, in first complete remission were initially included for propensity score matching. Following the matching process, 126 patients were selected for further analysis, with 42 patients in each of the auto‐HSCT, MSD‐HSCT, and AD‐HSCT groups. Among the AD‐HSCT group, 38 of 42 (90.5%) patients received haploidentical HSCT. In patients with persistent undetectable measurable residual disease (uMRD) before transplant (n = 83), overall survival (OS) was similar across the groups. However, auto‐HSCT showed a trend of increased disease‐free survival (DFS) compared to AD‐HSCT (HR 2.85, P = 0.09), resulting in a 3‐year DFS and OS of 79.1% and 82.8%, respectively. In the non‐persistent uMRD group (n = 38), auto‐HSCT exhibited a tendency to increase the risk of relapse, particularly when compared to AD‐HSCT (HR 0.24, P = 0.07), but this did not result in inferior OS. The monthly direct medical cost per patient within the first 2 years after HSCT was significantly lower in auto‐HSCT compared to MSD‐HSCT (P = 0.015) and AD‐HSCT (P < 0.001). Our results provide evidence for the use of auto‐HSCT as a viable therapeutic option for favorable‐ and intermediate‐risk de novo AML patients in first complete remission with persistent uMRD. Additionally, our findings demonstrated a notable cost advantage associated with auto‐HSCT compared to MSD‐HSCT and AD‐HSCT.
A case study on using an intrauterine foley catheter to reduce postpartum bleeding in a patient with hemophilia
Authors: Yared Georges , Tachdjian Annie , El Kazwini Mohammad El Jawad , Azzi Joelle , El Hajjar Charlotte , Ghazal Kariman 
Published: September 2023

SynopsisCase of a pregnant patient suffering from severe hemophilia A who had effective uterine foley tamponade to treat postpartum hemorrhage.