bluuebunny/crossref_metadata_2025_split huggingface dataset:
The impact of COVID-19 and bushfires on the mental health of Australian adolescents: a cross-sectional study
Authors: Beames Joanne R. , Huckvale Kit , Fujimoto Hiroko , Maston Kate , Batterham Philip J. , Calear Alison L. , Mackinnon Andrew , Werner-Seidler Aliza , Christensen Helen 
Published: March 2023

AbstractBackgroundWhen COVID-19 spread to Australia in January 2020, many communities were already in a state of emergency from the Black Summer bushfires. Studies of adolescent mental health have typically focused on the effects of COVID-19 in isolation. Few studies have examined the impact of COVID-19 and other co-occurring disasters, such as the Black Summer bushfires in Australia, on adolescent mental health.MethodsWe conducted a cross-sectional survey to examine the impact of COVID-19 and the Black Summer bushfires on the mental health of Australian adolescents. Participants (N = 5866; mean age 13.61 years) answered self-report questionnaires about COVID-19 diagnosis/quarantine (being diagnosed with and/or quarantined because of COVID-19) and personal exposure to bushfire harm (being physically injured, evacuated from home and/or having possessions destroyed). Validated standardised scales were used to assess depression, psychological distress, anxiety, insomnia, and suicidal ideation. Trauma related to COVID-19 and the bushfires was also assessed. The survey was completed in two large school-based cohorts between October 2020 and November 2021.ResultsExposure to COVID-19 diagnosis/quarantine was associated with increased probability of elevated trauma. Exposure to personal harm by the bushfires was associated with increased probability of elevated insomnia, suicidal ideation, and trauma. There were no interactive effects between disasters on adolescent mental health. Effects between personal risk factors and disasters were generally additive or sub-additive.ConclusionsAdolescent mental health responses to community-level disasters are multi-faceted. Complex psychosocial factors associated with mental ill health may be relevant irrespective of disaster. Future research is needed to investigate synergistic effects of disasters on young mental health.
The Network Zoo: a multilingual package for the inference and analysis of gene regulatory networks
Authors: Ben Guebila Marouen , Wang Tian , Lopes-Ramos Camila M. , Fanfani Viola , Weighill Des , Burkholz Rebekka , Schlauch Daniel , Paulson Joseph N. , Altenbuchinger Michael , Shutta Katherine H. , Sonawane Abhijeet R. , Lim James , Calderer Genis , van IJzendoorn David G.P. , Morgan Daniel , Marin Alessandro , Chen Cho-Yi , Song Qi , Saha Enakshi , DeMeo Dawn L. , Padi Megha , Platig John , Kuijjer Marieke L. , Glass Kimberly , Quackenbush John 
Published: March 2023

AbstractInference and analysis of gene regulatory networks (GRNs) require software that integrates multi-omic data from various sources. The Network Zoo (netZoo; netzoo.github.io) is a collection of open-source methods to infer GRNs, conduct differential network analyses, estimate community structure, and explore the transitions between biological states. The netZoo builds on our ongoing development of network methods, harmonizing the implementations in various computing languages and between methods to allow better integration of these tools into analytical pipelines. We demonstrate the utility using multi-omic data from the Cancer Cell Line Encyclopedia. We will continue to expand the netZoo to incorporate additional methods.
Hypochromic red cells as a prognostic indicator of survival among patients with systemic sclerosis screened for pulmonary hypertension
Authors: Xanthouli Panagiota , Gordjani Ojan , Benjamin Nicola , Harutyunova Satenik , Egenlauf Benjamin , Marra Alberto M. , Haas Simon , Milde Nicklas , Blank Norbert , Lorenz Hanns-Martin , Fiehn Christoph , Ulrich Silvia , Distler Oliver , Grünig Ekkehard , Eichstaedt Christina A. 
Published: March 2023

Abstract
Background
Patients with systemic sclerosis (SSc) are frequently affected by iron deficiency, particularly those with pulmonary hypertension (PH). The first data indicate the prognostic importance of hypochromic red cells (% HRC) > 2% among patients with PH. Hence, the objective of our study was to investigate the prognostic value of % HRC in SSc patients screened for PH.

Methods
In this retrospective, single-center cohort study, SSc patients with a screening for PH were enrolled. Clinical characteristics and laboratory and pulmonary functional parameters associated with the prognosis of SSc were analyzed using uni- and multivariable analysis.

Results
From 280 SSc patients screened, 171 could be included in the analysis having available data of iron metabolism (81% female, 60 ± 13 years of age, 77% limited cutaneous SSc, 65 manifest PH, and 73 pulmonary fibrosis). The patients were followed for 2.4 ± 1.8 (median 2.4) years. HRC > 2% at baseline was significantly associated with worse survival in the uni- (p = 0.018) and multivariable (p = 0.031) analysis independent from the presence of PH or pulmonary parenchymal manifestations. The combination of HRC > 2% and low diffusion capacity for carbon monoxide (DLCO) ≤ 65% predicted was significantly associated with survival (p < 0.0001).

Conclusion
This is the first study reporting that HRC > 2% is an independent prognostic predictor of mortality and can possibly be used as a biomarker among SSc patients. The combination of HRC > 2% and DLCO ≤ 65% predicted could serve in the risk stratification of SSc patients. Larger studies are required to confirm these findings.
Correlation between Selective Motor Control of the Lower Extremities and Balance in Spastic Hemiplegic Cerebral Palsy: a randomized controlled trial
Authors: Mohammed Amira H. , El-Serougy Hager R. , Karim Amel E Abdel , Sakr Mohamad , Sheha Samah M. 
Published: March 2023

Abstract
Background
Children with cerebral palsy (CP) have motor deficits caused by spasticity, weakness, contractures, diminished selective motor control (SMC), and poor balance. The purpose of the current study was to evaluate the influence of mirror feedback on lower extremity selective motor control and balance in children with hemiplegic cerebral palsy. Understanding the relationship between SMC and balance will help children with hemiplegic CP receive more appropriate therapies.

Methods
Forty-seven children of both sexes diagnosed with hemiplegic CP participated in the study. Group1 (Gr1 - control group) received conventional physical therapy training while group 2 (Gr2 - intervention group) received conventional physical therapy training in addition to bilateral lower extremity mirror therapy (MT). The primary outcome measure used was Selective Control Assessment of Lower Extremity scale (SCALE), while the secondary outcome measure was the Pediatric Balance Scale (PBS).

Results
There were significant differences in Selective Control Assessment of Lower Extremity Scale (SCALE) and Pediatric Balance Scale (PBS) between both groups in favor of Gr2. After treatment, both groups improved significantly, yet Gr2 outperformed Gr1 by a large margin.

Conclusion
Mirror therapy may be a useful addition to home-based motor interventions for children with hemiplegic CP due to its relative simplicity, low cost, and high patient adherence. Additionally, it may help children improve their selective motor skills and balance.

Trial registration
Current Controlled Trials using African Clinical Trials Registry website with ID number PACTR202105604636415 retrospectively registered on 21/01/202.
The electrocardiogram on the wrist: a frightening experience to the untrained consumer: a case report
Authors: Zenzes Michael , Seba Philip , Portocarrero Vivero-Fäh Bettina 
Published: March 2023

Abstract
Background
Smartwatches offering electrocardiogram recordings advertise the benefits of supporting an active and healthy lifestyle. More often, medical professionals are faced with privately acquired electrocardiogram data of undetermined quality recorded by smartwatches. This is boasted by results and suggestions for medical benefits, based on industry-sponsored trials and potentially biased case reports. Yet potential risks and adverse effects have been widely overlooked.

Case presentation
This case report describes an emergency consultation of a 27-year-old Swiss–German man lacking known previous medical conditions who developed an episode of anxiety and panic due to pain in the left chest prompted by over-interpretation of unremarkable electrocardiogram readings of his smartwatch. Fearing acute coronary syndrome, he presented at the emergency department. His smartwatch electrocardiograms, as well as a 12-lead electrocardiogram, appeared normal. After extensive calming and reassuring, as well as symptomatic therapy with paracetamol and lorazepam, the patient was discharged with no indications for further treatment.

Conclusions
This case demonstrates the potential risks of anxiety from nonprofessional electrocardiogram recordings by smartwatches. Medico–legal and practical aspects of electrocardiogram recordings by smartwatches need to be further considered. The case shows the potential side effects of pseudo-medical recommendations for the untrained consumer, and may add to the discussion on the ethics of how to evaluate smartwatch electrocardiogram data as a medical professional.
A novel Sybil attack detection scheme in mobile IoT based on collaborate edge computing
Authors: Yan Junwei , Jiang Tao , Lin Liwei , Wu Zhengyu , Ye Xiucai , Tian Mengke , Wang Yong 
Published: March 2023

Abstract
Background
Internet of things (IoT) has been used in many places. IoT make devices connected to the Internet via sensor devices to achieve the interconnection between things and things, people and things. Sybil attacker attacks IoT by imitating the identity of users. Few methods are applicable for mobile IoT in previous Sybil attack detecting methods, while the methods are mainly focus on static IoT.

Results
A distributive and lightweight Sybil attack detection scheme in the mobile IoT is proposed in this paper. This scheme works around received signal strength indications (RSSI). The scheme consists of two rounds. Identity information is sent from member nodes to edge nodes in both of the two rounds. In the first round edge, nodes calculate the possible RSSI interval for each member node; in the second round, they check the RSSI value of member nodes to detect Sybil attacks. Intelligent algorithms are used to predict the position of member nodes, which makes the theoretical interval more accurate. Extensive experimental studies show that in the true and false detection rate, this scheme is superior to many existing schemes.
Application research of polar coded OFDM underwater acoustic communications
Authors: Zhai Yushuang , Li Jilong , Feng Haihong , Hong Feng 
Published: March 2023

AbstractTo further accelerate the practical process of polar codes in underwater acoustic communication (UWC) and improve the reliability and efficiency of underwater data transmission, we first propose a polar code construction method suitable for the underwater acoustic channel, which drastically reduces the complexity and meets the need for practical UWC. Then, we establish a practical polar coded UWC scheme by devising a two-step procedure based on the Orthogonal Frequency Division Multiplexing technique. Moreover, through simulation, the scheme's performances with the unimproved and the proposed polar code construction method are analyzed. Moreover, its performances with four different polar decoders are also given. The performance comparison with the LDPC coded UWC system is studied as well. Furthermore, lake-trial results under two different channel conditions demonstrate that the proposed construction method and the scheme effectively guarantee the reliability of data transmission, achieving error-free transmission at a transmission distance of 1718 m with the signal-to-noise-ratio (SNR) of 21 dB, and a transmission distance of 749 m with the SNR of 16.5 dB, better than the LDPC coded system with same conditions. The semi-experimental results show that the proposed polar coded system under the Cyclic Redundancy Check-Aided Successive Cancellation List decoder outperforms the LDPC coded system by approximately 2.1–3.7 dB.
Fundamental and speculative components of the cryptocurrency pricing dynamics
Authors: Kukacka Jiri , Kristoufek Ladislav 
Published: March 2023

AbstractThe driving forces behind cryptoassets’ price dynamics are often perceived as being dominated by speculative factors and inherent bubble-bust episodes. Fundamental components are believed to have a weak, if any, role in the price-formation process. This study examines five cryptoassets with different backgrounds, namely Bitcoin, Ethereum, Litecoin, XRP, and Dogecoin between 2016 and 2022. It utilizes the cusp catastrophe model to connect the fundamental and speculative drivers with possible price bifurcation characteristics of market collapse events. The findings show that the price and return dynamics of all the studied assets, except for Dogecoin, emerge from complex interactions between fundamental and speculative components, including episodes of price bifurcations. Bitcoin shows the strongest fundamentals, with on-chain activity and economic factors driving the fundamental part of the dynamics. Investor attention and off-chain activity drive the speculative component for all studied assets. Among the fundamental drivers, the analyzed cryptoassets present their coin-specific factors, which can be tracked to their protocol specifics and are economically sound.
Acute organ injury and long-term sequelae of severe pneumococcal infections
Authors: Kruckow Katherine L. , Zhao Kevin , Bowdish Dawn M.E. , Orihuela Carlos J. 
Published: March 2023

AbstractStreptococcus pneumoniae(Spn) is a major public health problem, as it is a main cause of otitis media, community-acquired pneumonia, bacteremia, sepsis, and meningitis. Acute episodes of pneumococcal disease have been demonstrated to cause organ damage with lingering negative consequences. Cytotoxic products released by the bacterium, biomechanical and physiological stress resulting from infection, and the corresponding inflammatory response together contribute to organ damage accrued during infection. The collective result of this damage can be acutely life-threatening, but among survivors, it also contributes to the long-lasting sequelae of pneumococcal disease. These include the development of new morbidities or exacerbation of pre-existing conditions such as COPD, heart disease, and neurological impairments. Currently, pneumonia is ranked as the 9thleading cause of death, but this estimate only considers short-term mortality and likely underestimates the true long-term impact of disease. Herein, we review the data that indicates damage incurred during acute pneumococcal infection can result in long-term sequelae which reduces quality of life and life expectancy among pneumococcal disease survivors.
Gallic acid enhances anti-lymphoma function of anti-CD19 CAR-T cells in vitro and in vivo
Authors: Luo Zhiqiang , Shi Jiaru , Jiang Qiyao , Yu Guohua , Li Xiaorui , Yu Zhuoying , Wang Jianxun , Shi Yuanyuan 
Published: March 2023

AbstractChimeric antigen receptor T (CAR-T) cell targeting CD19 antigen has achieved exhilarative clinical efficacy in B-cell malignancies. However, challenges still remain for the currently approved anti-CD19 CAR-T therapies, including high recurrence rates, side effects and resistance. Herein, we aim to explore combinatorial therapy by use of anti-CD19 CAR-T immunotherapy and gallic acid (GA, an immunomodulatory natural product) for improving treatment efficacy. We assessed the combinatorial effect of anti-CD19 CAR-T immunotherapy with GA in cell models and a tumor-bearing mice model. Then, the underlying mechanism of GA on CAR-T cells were investigated by integrating network pharmacology, RNA-seq analysis and experimental validation. Furthermore, the potential direct targets of GA on CAR-T cells were explored by integrating molecular docking analysis with surface plasmon resonance (SPR) assay. The results showed that GA significantly enhanced the anti-tumor effects, cytokine production as well as the expansion of anti-CD19 CAR-T cells, which may be mainly through the activation of IL4/JAK3-STAT3 signaling pathway. Furthermore, GA may directly target and activate STAT3, which may, at least in part, contribute to STAT3 activation. Overall, the findings reported here suggested that the combination of anti-CD19 CAR-T immunotherapy with GA would be a promising approach to increase the anti-lymphoma efficacy.
Actin-related protein 6 facilitates proneural protein-induced gene activation for rapid neural differentiation
Authors: Hsiao Yun-Ling , Chen Hui-Wen , Chen Kuan-Han , Tan Bertrand Chin-Ming , Chen Chia-Hsiang , Pi Haiwei 
Published: March 2023

ABSTRACT
Neurogenesis is initiated by basic helix-loop-helix proneural proteins. Here, we show that Actin-related protein 6 (Arp6), a core component of the H2A.Z exchange complex SWR1, interacts with proneural proteins and is crucial for efficient onset of proneural protein target gene expression. Arp6 mutants exhibit reduced transcription in sensory organ precursors (SOPs) downstream of the proneural protein patterning event. This leads to retarded differentiation and division of SOPs and smaller sensory organs. These phenotypes are also observed in proneural gene hypomorphic mutants. Proneural protein expression is not reduced in Arp6 mutants. Enhanced proneural gene expression fails to rescue retarded differentiation in Arp6 mutants, suggesting that Arp6 acts downstream of or in parallel with proneural proteins. H2A.Z mutants display Arp6-like retardation in SOPs. Transcriptomic analyses demonstrate that loss of Arp6 and H2A.Z preferentially decreases expression of proneural protein-activated genes. H2A.Z enrichment in nucleosomes around the transcription start site before neurogenesis correlates highly with greater activation of proneural protein target genes by H2A.Z. We propose that upon proneural protein binding to E-box sites, H2A.Z incorporation around the transcription start site allows rapid and efficient activation of target genes, promoting rapid neural differentiation.
Responses of the in vitro turtle brain to visual and auditory stimuli during severe hypoxia
Authors: Ariel Michael , Ahuja Shivika , Warren Daniel E. 
Published: March 2023

ABSTRACT
North American pond turtles (Emydidae) are renowned for their ability to survive extreme hypoxia and anoxia, which enables several species to overwinter in ice-locked, anoxic freshwater ponds and bogs for months. Centrally important for surviving these conditions is a profound metabolic suppression, which enables ATP demands to be met entirely with glycolysis. To better understand whether anoxia limits special sensory functions, we recorded evoked potentials in a reduced brain preparation, in vitro, that was perfused with severely hypoxic artificial cerebral spinal fluid (aCSF). For recordings of visual responses, an LED was flashed onto retinal eyecups while evoked potentials were recorded from the retina or the optic tectum. For recordings of auditory responses, a piezomotor-controlled glass actuator displaced the tympanic membrane while evoked potentials were recorded from the cochlear nuclei. We found that visual responses decreased when perfused with hypoxic perfusate (aCSF PO2&lt;4.0 kPa). In contrast, the evoked response within the cochlear nuclei was unattenuated. These data provide further support that pond turtles have a limited ability to sense visual information in their environment even while moderately hypoxic, but that auditory input may become a principal avenue of sensory perception during extreme diving in this species such as occurs during anoxic submergence.
Impact of the Covid-19 pandemic on women entrepreneurs in Sukoharjo Regency
Authors: Kriseka Putri Mediany , Annisa Diya 
Published: March 2023

Background: The number of workers in the Sukoharjo Regency in 2020 decreased compared to 2019 due to the Covid-19 pandemic. This is different from the number of MSME actors in Sukoharjo Regency, which has increased after the pandemic. During the Covid-19 pandemic, women entrepreneurs are experiencing a drastic impact on their lives. Most women are encouraged to become entrepreneurs, because their income is not enough if they only manage housework and take care of children. Methods: This study uses semi-structured interviews and focus group discussions under a case study research design. The main resource persons involved were seven business actors in the micro sector who were female and as a supporting resource in this study was one person from the Cooperative Small and Medium Enterprises, and Trade Office of Sukoharjo Regency. Results: The results of the study provide a valuable overview of how the Covid-19 pandemic affects women entrepreneurs with aspects of household income, lifestyle, mental health, and sales of the business. Since the Covid-19 pandemic, household income has decreased. Likewise, the lifestyles of women entrepreneurs have changed. They struggled to meet the basic needs of their families and a decline in their standard of living when compared to before the Covid-19 pandemic. Most of the women entrepreneurs admitted that their mental health was affected by the unprecedented changes in conditions ever. Then business sales also decreased, this was supported by a decrease in the purchasing power of customers so it could threaten the existence of their business. Conclusions: The results of this study are expected to be used to evaluate how women entrepreneurs can survive in any crisis conditions. In addition, the research results on the impact of the Covid-19 pandemic indicate the need to reconsider innovation, digitalization, understanding of business management and self-management in maintaining business continuity.
The identification of retro-DNAs in primate genomes as DNA transposons mobilizing via retrotransposition
Authors: Tang Wangxiangfu , Liang Ping 
Published: March 2023

Background: Mobile elements (MEs) constitute a major portion of the genome in primates and other higher eukaryotes, and they play important role in genome evolution and gene function. MEs can be divided into two fundamentally different classes: DNA transposons which transpose in the genome in a “cut-and-paste” style, and retrotransposons which propagate in a “copy-and-paste” fashion via a process involving transcription and reverse-transcription. In primate genomes, DNA transposons are mostly dead, while many retrotransposons are still highly active. We report here the identification of a new type of MEs, which we call “retro-DNAs”, for their combined characteristics of these two fundamentally different ME classes. Methods: A comparative computational genomic approach was used to analyze the reference genome sequences of 10 primate species consisting of five apes, four monkeys, and marmoset. Results: From our analysis, we identified a total of 1,750 retro-DNAs, representing 748 unique insertion events in the genomes of ten primate species including human. These retro-DNAs contain sequences of DNA transposons but lack the terminal inverted repeats (TIRs), the hallmark of DNA transposons. Instead, they show characteristics of retrotransposons, such as polyA tails, longer target-site duplications (TSDs), and the “TT/AAAA” insertion site motif, suggesting the use of the L1-based target-primed reverse transcription (TPRT) mechanism. At least 40% of these retro-DNAs locate into genic regions, presenting potentials for impacting gene function. More interestingly, some retro-DNAs, as well as their parent sites, show certain levels of expression, suggesting that they have the potential to create more retro-DNA copies in the present primate genomes. Conclusions: Although small in number, the identification of these retro-DNAs reveals a new mechanism for propagating DNA transposons in primate genomes without active canonical DNA transposon activity. Our data also suggest that the TPRT machinery may transpose a wider variety of DNA sequences in the genomes.