bluuebunny/crossref_metadata_2025_split huggingface dataset:
Translation and Psychometric Evaluation of the Persian Version of “Good Death Inventory- Short Form” from the perspective of family- members of cancer patients
Authors: Roshan Hosein Mohammadi , Ebadi Abbas , Karimi Leila , Barasteh Salman 
Published: March 2023

Abstract
Introduction: Achieving good death and enhancement of end-of-life care are among the objectives of palliative care in patients with cancer. There should be an instrument for evaluating the quality of palliative care provided by family members to provide comprehensive care at the end of life. This study was done for translation and assessment the psychometric properties of good death inventory- short form according to the perspective of family of patients with cancer in Tehran.
Method: This methodological study was done in 2022 at two hospitals in Tehran. The translation was done after receiving permission from the instrument’s developer via forward-backward method. Face validity was examined through cognitive interviewing with 10 family members of patients with cancer. To perform the quantitative and qualitative content validation, the opinions of 10 palliative care specialists were used. The construct validity was explored through exploratory factor analysis and examination of convergent validation with care evaluation scale 2.0 (CES), as well as inspection of correlation by answering two general questions of satisfaction with treatment and end-stage quality of life. The scale’s reliability, internal consistency was calculated using Cronbach’s alpha coefficient and stability via test-retest.
Results:Overall, 204 family members of patients with cancer were included. In the exploratory factor analysis, three factors of peace, hope, and value as well as quality of care were extracted with cumulative variance of 41.8%. We found a significant and suitable correlation between the total scores of the participants Good death inventory-short form and care evaluation scale2.0 (r = 0.459, P < 0.001). Also, there was a positive and significant correlation between the GDI short form total score and general satisfaction with end-of-life care (r=0.423, p<0.001) as well as the patient’s general quality of life at terminal stages (r=0.539, p<0.001). The Cronbach’s alpha coefficient for the questionnaire was found 0.842, and the stability was confirmed with ICC=0.851.
Conclusion: the Persian version of GDI-short form is a valid and reliable questionnaire which can investigate the factors associated with good death according to patients’ family members’ perspective. Thus, this instrument can be used in clinical evaluation as well as research purposes and for family members in Iranian population.
Experimental comparative study on Near-dry wire-cut electrical discharge machining process using moistened wire
Authors: Xie Wenling , Guo Cuixia , Wang Xing , Zhang Jianping 
Published: March 2023

Abstract

The disturbance of flushing to the electrode wire exists in conventional top and bottom flushing process and the machined surface easily being burned in the dry process, a novel dielectric fluid feeding method (moistened wire method) with near-dry high-speed reciprocating wire electrical discharge machine (HS-WEDM) was proposed. A dielectric fluid was poured onto a drum to achieve a moistened wire and packed into the discharge gap by a high-speed running molybdenum wire, and form a gas-liquid double-layer dielectric for discharge machining, cooling and cleaning for the working material. The effect of four processing (conventional top and bottom flushing with emulsion, the dry with atmospheric, the moistened wire with emulsion and moistened wire with emulsion-SiC nanofluids) during the finishing process on the kerf width, average cutting speed, surface roughness, surface morphology, and corner accuracy of the Cr12MoV die steel was studied. Compared to the conventional top and bottom flushingprocess, the moistened wires with emulsion and emulsion-SiC nanofluid processes maintained the high cutting speeds and obtained smooth machined surfaces. The Ra values decreased by 39.8% and 45.8%, the inner circle radius decreased by 4.1% and 4.9%, and outer circle radius decreased by 3.4% and 10.3%, respectively. The results showed that the moistened wire process improved the machined surface quality and corner accuracy of 90° profile without sacrificing the average cutting speed due to their moderate discharge gaps. Compared with the moistened wires with emulsion process, the moistened wires with emulsion-SiC nanofluid process further improved machined quality with lower surface roughness and corner error.
lncRNA TRHDE-AS1 correlated with genomic landscape and clinical outcome in glioma
Authors: Xie Jinxuan , Lin Yi , Li Yajie , Fang Aizhong , Li Xin , Li Wenbin 
Published: March 2023

Abstract
Background
The role of lncRNA in cancer development has received more and more attention in researches. A variety of lncRNAs are associated with the occurrence and development of glioma. However, the role of TRHDE-AS1 in glioma is still unknown.
Result
In this study, we explored the role of TRHDE-AS1 in glioma through bioinformatics methods. We first identified an association between TRHDE-AS1 and tumor prognosis in a pan-cancer analysis. Subsequently, the expression levels of TRHDE-AS1 in various clinical types of glioma were compared, and significant differences were found in pathological classification, WHO classification, molecular classification, IDH mutation and age stratification. We analyzed the genes co-expressed with TRHDE-AS1 in glioma. In the functional analysis of TRHDE-AS1, we found that TRHDE-AS1 may be involved in the regulation of synapse-related functions. In glioma cancer driver gene correlation analysis, it was also found that TRHDE-AS1 was significantly correlated with the expression levels of multiple driver genes such as TP53, BRAF, and IDH1. By comparing the mutant profiles of the high and low TRHDE-AS1 groups, we also found that there may be differences in TP53 and CIC gene mutations in low-grade gliomas. Subsequent correlation analysis between TRHDE-AS1 and glioma immune microenvironment showed that the expression level of TRHDE-AS1 was correlated with a variety of immune cells.
Conclusion
Therefore, we believe that TRHDE-AS1 is involved in the occurrence and development of glioma and has the ability to predict the prognosis of glioma as a biomarker of glioma.
Development of a Genetically Encoded Magnetic Platform in Magnetospirillum gryphiswaldense MSR-1 for Downstream Processing of Protein Expression System
Authors: Wu Sha , Tian Jiesheng , Xue Xianle , Tang Zongwen , Huang Zekai , Hammock Bruce D. , Morisseau Christophe , Li Qing X. , Xu Ting 
Published: March 2023

Abstract
Background: Protein downstream processing remains a challenge in protein production, especially in low yields of products, in spite of ensuring effective disruption of cell and separation of target proteins. It is complicated, expensive and time-consuming. Here, we report a novel nano-bio-purification system for producing recombinant proteins of interest with automatic purification from engineered bacteria.
Results: This system employed a complete genetic engineering downstream processing platform for proteins at low expression levels, referred to as a genetically encoded magnetic platform (GEMP). GEMP consists of four elements as follows. (1) A truncated phage lambda lysis cassette (RRz/Rz1) is controllable for lysis of Magnetospirillum gryphiswaldense MSR-1 (host cell). (2) A surface-expressed nuclease (NucA) is to reduce viscosity of homogenate by hydrolyzing long chain nucleic acids. (3) A bacteriogenic magnetic nanoparticle, known as magnetosome, allows an easy separation system in a magnetic field. (4) An intein realizes abscission of products (nanobodies against tetrabromobisphenol A) from magnetosome.
Conclusions: In this work, removal of most impurities greatly simplified the subsequent purification procedure. The system also facilitated the bioproduction of nanomaterials. The developed platform can substantially simplify industrial protein production and reduce its cost.
Energetics of the non-covalent interactions in the acetonechloroform system by MP2/CBS quantum chemical calculations
Authors: Rosas-Garcia Victor M. , Alanís-Cuevas Cristofer Adrián 
Published: March 2023

Abstract
We studied the energetics of the non-covalent interactions present in the acetone-chloroform system at the MP2/Complete Basis Set level of theory to shed light on the interactions responsible for azeotrope formation. To this end, we did a configurational search for the diverse geometries of homodimers of (acetone)2 and (chloroform)2 , and acetone-chloroform heterodimers. All the structures were subjected to full geometry optimization and their vibrational frequencies calculated. Energies were extrapolated to the Complete Basis Set limit and BSSE correction applied. We found that MP2/Complete Basis Set level of theory predicts the trend (acetone)2 > acetone-chloroform > (chloroform)2 , while MP2/cc-pVDZ severely underestimates the interaction energies as well as predicts a wrong trend in the energies. The resulting wavefunctions were analyzed by Reduced Density Gradient and Non-Covalent Interaction methods yielding a variety of critical points. This revealed that the reported hydrogen bond for the acetone-chloroform dimer likely has a van der Waals component involving interaction between a chlorine in chloroform and a hydrogen in acetone.
A time-motion study of community health workers delivering community-based primary health care in Neno District, Malawi
Authors: Aron Moses Banda , Ndambo Myness Kasanda , Munyaneza Fabien , Mulwafu Manuel , Makungwa Henry , Nhlema Basimenye , Connolly Emilia 
Published: March 2023

Abstract
Introduction: Community Health Workers (CHWs) are vital resources in delivering community-based primary health care, especially in low-and-middle-income countries (LMIC). However, few studies have investigated detailed time and task assessments of CHW's work. We conducted a time-motion study to evaluate CHWs' time on health conditions and specific tasks in Neno District, Malawi.
Methods: We conducted a descriptive quantitative study utilizing a time observation tracker to capture time spent by CHWs on focused health conditions and tasks performed during household visits. We observed 64 CHWs between 29 June and 20 August 2020. We computed counts and median to describe CHW distribution, visit type, and time spent per health condition and task. We utilized Mood’s Median Test to compare the median time spent at a household during monthly visits with the program design standard time. We used Pairwise Median Test to test differences in median time duration for health conditions and assigned tasks.
Results: We observed 660 CHW visits from 64 CHWs, with 95.2% (n= 628) of the visits as monthly household visits. The median time for a monthly household visit was 34 minutes, statistically less than the program design time of 60 minutes (p<0.001). While the CHW program focused on eight disease areas, pretesting with the observation tool showed that CHWs were engaged in additional health areas like COVID-19. Of the 3043 health area touches by CHWs observed, COVID-19, tuberculosis, and non-communicable diseases (NCDs) had the highest touches (19.3%, 17.6%, and 16.6%, respectively). The median time spent on sexually transmitted infections (STIs) and NCDs was statistically higher than in other health areas (p<0.05). Of 3813 tasks completed by CHWs, 1640 (43%) were on health education and promotion. A significant difference was observed in the median time spent on health education, promotion, and screening compared to other tasks (p<0.05).
Conclusion: This study demonstrates that CHWs spend the most time on health education, promotion, and screening per programmatic objectives but, overall, less time than program design. CHWs deliver care for a broader range of health conditions than the programmatic design indicates. Future studies should examine associations between time spent and quality of care delivery.
Quantifying the invasion risk of West Nile virus: insights from a multi-vector/multi host SEIR model
Authors: Ferraguti Martina , Martins Afonso Dimas , Artzy-Randrup Yael 
Published: March 2023

Abstract
The invasion of vector-borne diseases depends on the type of specific features of the vector and hosts at play. Within the Culex pipiens complex, differences in ecology, biology, and vector competence can influence the risk of West Nile virus (WNV) outbreaks. To determine which life-history traits are most important, we constructed an epidemiological Susceptible-Exposed-Infectious-Recovered model with three vector eco-types, Culex pipiens pipiens, Cx. pip. molestus, and their hybrids, and two vertebrate hosts, birds (as amplifying hosts) and humans (as dead-end hosts). We investigated how differences in feeding preferences and transmission rates influenced WNV transmission across different habitats and two simulated scenarios, i.e., global change and increasing urbanisation settings, to investigate the impact of increasing mosquito and human abundance on the WNV transmission risk. Our results showed that vector feeding preferences and the transmission rate between mosquitoes and birds were the parameters that most impacted WNV invasion risk. Natural habitats were overall more susceptible to WNV invasion, although rural habitats were also susceptible in a climate change scenario. Pipiens-type-related factors dominated virus amplification in natural and rural habitats, while molestus-type-related factors drove the spread of WNV in urban environments. Contrary to common opinion, our findings suggest that hybrids may play only a minor role in WNV transmission. Our study will improve current vector surveillance and control programs by targeting specific vector types in specific environments, especially in rural settings, which are most responsive to environmental shifts. This approach can help reduce wasted time and economic costs while maximizing the efficiency of local public health authorities.
Investigating the Atlantic-monsoon teleconnection pathways in PMIP3 Last Millennium Simulations
Authors: Dutta Arijeet , Sivankutty Rahul , Neena J.M. 
Published: March 2023

Abstract
The Atlantic multidecadal oscillation (AMO) is considered as one of the major drivers of Indian Summer monsoon (ISM) multidecadal variability, but the exact mechanism of teleconnection remains elusive. AMO can impact the ISM dominantly by two pathways – by generating upperlevel circulation and heating responses across Eurasia and via the Pacific through the atmospheric bridge mechanism and associated modulations of Hadley-Walker circulations in the Indo-Pacific. Using the PMIP3 Last millennium (LM) simulations, the current study investigates the AMO-ISM teleconnection in the context of these pathways of interaction. A significant positive correlation is observed between AMO and ISM in five of the eight models. While the models display only limited skill in simulating the upper-level circulation and tropospheric temperature responses involved in the Eurasian pathway, they exhibit better skill in capturing the Pacific SST responses induced by the AMO. Four of the five models capture the AMO modulation of summer North Atlantic Oscillation (SNAO), but major discrepancies are observed in the SNAO downstream responses. CCSM4 and MPI exhibit better skill in simulating the AMO forced upper-level circulation responses across Eurasia, while CSIRO, GISS and MRI-CGCM3 exhibit better skill in simulating the AMO modulation of extra tropical-tropical SST gradient over the Pacific. Reliability of decadal climate predictions of ISM largely depends on the fidelity of current generation global models in simulating the teleconnection mechanisms which modulate the ISM multidecadal variability, and results from the current study helps in highlighting some of the main deficiencies in model simulated teleconnection processes.
Phylogenotyping of the Vietnamese infectious bursal disease viruses according to the newly unified genotypic classification scheme
Authors: LE THANH HOA , Le Xuyen Thi Kim , Do Roan Thi , Doan Huong Thi Thanh , Nguyen Khue Thi , Pham Linh Thi Khanh 
Published: March 2023

Abstract
The unified genotypic classification scheme (genotypes A0, A1–A8, and B1–B5) was proposed and applied for the classification of the infectious bursal disease virus (IBDV). Since 1987, IBDVs have circulated and evolved in Vietnam, but little is known about the genotypes present. The IBDV samples were collected in 1987, 2001–2006, 2008, 2011, 2015–2019, and 2021 in 18 provinces. We conducted phylogenotyping analysis based on the alignment of 143 VP2-HVR (439 bp, nt 628–1066; aa 210–355 in VP2), including 64 Vietnamese isolates and two vaccines, and 82 VP1 B-marker (725 bp, nt 13–737; aa 4–245 in VP1) sequences, including four Vietnamese isolates and one vaccine. The analysis revealed three A-genotypes, A1, A3, and A7, and two B-genotypes, B1 and B3, among the Vietnamese IBDVs. The IBDV strains showed the lowest average evolutionary distance at 8.6% between A1 and A3 and the highest (21.7%) between A5 and A7, while 14% was between B1 and B3 and 17% between B3 and B2. The signature residues 222T - 253Q - 256V - 294L - 299(S/N) appeared to be unique for the genotype A2; the 222A - 253Q - 256I - 294I - 299S for the A3, the 222T - 253Q - 256V - 294L - 299N for the A5, the 222Q - 253E - 256K - 294L - 299S for the A6, and the 222A - 253Q - 256A - 294L - 299S for the A8, and each of these could be the genotypical motif for genotypic discrimination. The timeline statistical summary revealed that the A3-genotype predominated (79.8% presence) in Vietnam from 1987 to 2021 and that it has remained dominant in circulating IBDVs over the last five years (2016–2021).
Engineering siRNA-loaded and RGDfC-targeted selenium nanoparticles for highly efficient silencing of DCBLD2 gene for colorectal cancer treatment
Authors: Huang Hongli , Chen Hanqing , Shou Diwen , Quan Ying , Cheng Jiemin , Chen Huiting , Ning Gang , Li Yongqiang , Xia Yu , Zhou Yongjian 
Published: March 2023

Abstract
Effective and safe delivery of small interfering RNA (siRNA) by nanomaterials to cancer cells is one of the main challenges in cancer treatment. In this study, we constructed the selenium nanoparticles conjugated with RGDfC (one tumor-targeted polypeptide) to prepare a biocompatible gene vector (RGDfC-SeNPs) and then loaded with siDCBLD2 to synthesize the RGDfC-Se@siDCBLD2 for colorectal cancer (CRC) therapy. As expected, RGDfC-SeNPs could enhance the cellular uptake of siDCBLD2 in human HCT-116 colon cancer cells by targeting polypeptide RGDfC on the surface of colon cancer cells. RGDfC-Se@siDCBLD2 could be effectively internalized by HCT-116 cells mainly through a clathrin-related endocytosis pathway. In addition, RGDfC-Se@siDCBLD2 exhibited high siRNA release efficiency in an acidic tumor environment. Moreover, RGDfC-Se@siDCBLD2 could inhibit the proliferation and induce apoptosis in HCT-116 cells by special silencing gene DCBLD2 expression. RGDfC-Se@siDCBLD2 could be specifically accumulated to the tumor sites and exhibited significantly anti-CRC efficacy on HCT-116 tumor-bearing mice without obvious side effects. Taken together, these results suggest that selenium nanoparticles can be used as an effective gene vector with good biocompatibility, and RGDfC-Se@siDCBLD2 provides a promising strategy for combining tumor-target and siRNA delivery in treating CRC.
Carbonation and sweet taste dysgeusia with Topiramate therapy for migraine prevention treatment: a case report
Authors: Galassi Luca , Facchinetti Federica 
Published: March 2023

Abstract
Introduction
Migraine is a common pathology with a reported incidence up to 12% in the European general population. It can determine a significant impact on patients’ quality of life considering symptoms that can range from headaches, nausea, vomiting or external stimuli hypersensibility.
Migraine treatment includes numerous classes of drugs that can be used both for treatment and prophylaxis including anti-epilepsy drugs such as Topiramate.
Although topiramate has been reported to be associated with a significant number of adverse side effects including dysgeusia. We report the first case of carbonated drink dysgeusia associated with sweet taste perversion.
Case presentation
We present the case of a 30 year-old man with aura migraine that developed carbonation and sweet taste dysgeusia associated with topiramate use.
After the beginning of therapy and reaching the titration of topiramate 100 mg/daily with the complete absence of migraine recurrence, the patient developed dysgeusia for carbonated beverages that started to feel flat and assume a sweet aftertaste, while other sweetened carbonated beverages tasted flat and unsweetened, determining a major impact on his social quality of life and low therapy adherence.
In order to better tolerate therapy, considering the satisfactory results obtained in terms of migraine relapse, the patient was sent for a psychological evaluation and counseling, eventually accepting his condition and to continue therapy.
Conclusions
Although taste misperceptions are often underestimated and causes are still not well defined, it’s mandatory to correctly inform patients on the possible side effects that could influence patients’ quality of life and therapy adherence.
A Meta-analysis Reveals Gastric Microbes Associated with Atrophic Gastritis
Authors: Gao Xiaoxiang , Yin Pingping , Ren Yilin , Yu Leilei , Tian Fengwei , Zhao Jianxin , Chen Wei , Xue Yuzheng , Zhai Qixiao 
Published: March 2023

Abstract
Previous studies have associated microbial alterations with gastritis; however, problems regarding the reproducibility of biomarkers in cohorts remain poorly resolved. Patients from different regions provided excellent information, that was conducive to the exploration of the gastric microbes associated with gastric inflammation. We performed a meta-analysis of seven reliable datasets, consisting of a total of 367 fecal 16s rRNA including atrophic gastritis (AG) (174 patients) and superficial gastritis (SG) (170 patients) after quality control and discarding samples of gastric fluid, since the microbial diversity between gastric fluid and antrum was different. We also investigated the similarity in microbial composition between healthy controls and SG without Helicobacter pylori infection, but not in those with H. pylori infection. According to the linear discriminant analysis effect size and random forest analyses, Bacteroides, Weissella, Actinomyces, Atopobium, Oribacterium, Peptostreptococcus, and Rothia were biomarkers between SG and AG (AG_N) without H. pylori infection, whereas Actinomyces, Cutibacterium, Helicobacter, Novosphingobium, Pseudomonas, and Streptococcus were signatures between SG and AG (AG_P) with H. pylori infection. We also found that the development of AG_N was associated with lower vitamin B12 levels, whereas significant changes in the gastric microbiota functions of AG_P included gallate, amino acid degradation, and heme biosynthesis. In addition, non-dialysis classifiers were developed for AG_N with an area under the curve (AUC) of 0.7417 and for AG_P with an AUC of 0.8862. This study thoroughly profiled the gastric microbiota of AG and SG and provided evidence of the potential of microbial markers as noninvasive classifiers for global AG.