bluuebunny/crossref_metadata_2025_split huggingface dataset:
Access to malaria diagnostic testing, anti-malarial treatment and long-lasting insecticidal nets among Immigrant workers in Yala province, southern Thailand
Authors:   , Naing Tinzar 
Published: September 2023

Background: In Southeast Asia, trans-border migration from neighboring countries into Thailand is a well-known phenomenon. Population movement and migration is a factor having significant implications for vector-borne disease transmissions.
Objectives: This study aims to assess migrants’ access to malaria diagnosis and treatment in Yala province who have had fever in the previous three months and to evaluate the coverage and utilization of ITNs (insecticidal nets).
Methodology: A survey was conducted among 414 immigrant workers, in which information was sought on socio-demography, history of fever, health seeking behaviours, net ownership and utilization. Survey analysis was employed. Results: As key findings, 36.5% (166) migrants got fever in the last 3 months, 51.3% (89) sought healthcare at health facilities, 44.9% (80) got tested for malaria, 19.1% (36) were malaria positive and 60.3% (22) could receive anti-malarial treatment. When seeking healthcare, 48.3% (43) were through village health volunteers and 46.1% (41) migrants went to malaria posts and health promoting hospitals. Regarding nets, 40.7% (181) owned ITNs, but only 34.7% (57) used ITNs every night. Of the surveyed population, 23.6% (116) were forest-goers, 64.7% (75) had fever in the last 3 months and 31.8% (24) were malaria positive. Fifty percent (59) forest goers owned ITNs, but 28.1% (33) used ITNs while in the forest. Conclusions: More than half of migrants could access to malaria diagnosis and testing though ITN utilization was low. Most of the migrants sought diagnosis and treatment via community-based malaria services under National Malaria Elimination Program. BCC (Behaviour Change Communication) activities need to be enhanced to improve migrants’ behaviours since only one-third of migrants who owned ITNs used ITNs every night.
Graph representation for room layout matching using spectral embedding
Authors:   , Sa-ngawong Thamonwan 
Published: September 2023

Graph matching is efficient to search similar layout when the architectural floor plan data size is increasing. Because the computational time of floor plan matching using spectral embedding is only in seconds, so it is one of the popular methods. However, the isomorphism of each floor plan leads to low accuracy in the matching process and it becomes the weakness of this method. Therefore, we propose a graph representation for room layout matching using spectral embedding. Normally, graph representations of the floor plan define nodes as rooms and edges as connections between rooms. Besides, the graph spectral embedding is to find the feature vector of each floor plan by ignoring the semantic of rooms. Our proposed method also considers both room semantic which is the connection between the area outside and inside the room, and the structure of each layout. Furthermore, we show that by adding an extra node, our method can handle the isomorphism of a graph based on a mathematical idea called eigenvalue testing. There are three main processes in the proposed method: floor plan extracting, appended topology graph and floor plan matching. The performance from our experiment shows that our proposed method improve the matched accuracy from the conventional method by about 27.81 percent.
Automatic Method for Avian Red Blood Cell and Heterophil Counting Using Iterative Thresholding
Authors:   , Autaiem Tanapat 
Published: September 2023

Thailand is one of the top broiler exporters. Red blood cell (RBC) and white blood cell (WBC) counting is one of the most basic methods for health screening process. Because the mammalian and avian blood is different, the mammalian blood analyzing techniques cannot be used, so the counting is done manually. This thesis proposes an automatic counting method for avian RBCs and heterophils, the most common type of avian WBC. The detection of avian RBC is challenging because of elliptic nucleated cell, the possibility of overlapped cells, and various staining. Otsu’s multiple thresholding method is used to automatically extract nuclei under different staining. The threshold value is then reduced iteratively to separate connected nuclei. Heterophil detection is done by removing the detected RBCs, using edge information to extract possible WBCs, then applying statistically based algorithm to filter heterophils. The results show that RBC counting error is less than clinical acceptable error of 5% for 4 out of 5 types of slide and heterophil counting error is 8.8%.
Influence of curved root canal on retreatment efficacy of bioceramic sealer
Authors:   , Sujaritwanit Kasemsun 
Published: September 2023

This study evaluated the influence of a curved root canal on the retreatment efficacy of root canals sealed with bioceramic sealer. 60 mandibular molars were divided into 4 subgroups of severely curved canal filled with AH Plus, slightly curved canal filled with AH Plus, severely curved canal filled with BioRoot RCS and slightly curved canal filled with BioRoot. Two weeks after root canal obturation, retreatment was performed with WaveOne Gold Primary and ProTaper Next X3 following with passive ultrasonic irrigation. The retreatment efficacy parameters were regaining canal patency, retreatment time and residual filling material. MicroCT and confocal microscope were used to evaluate the residual filling materials and sealer penetration in dentinal tubules. Regaining canal patency was compared by Pearson Chi-square test. Retreatment time and residual filling materials were compared using one-way ANOVA at 0.05 level of confidence. Retreatment efficacy of BioRoot RCS in severely curved root canal was similar to that of slightly curved root canal. There was no significant difference in retreatment efficacy between BioRoot RCS and AH Plus groups, except for regaining canal patency.
Functional characterization of cytosolic ddx41 sensor in mediating antiviral immune response in black tiger shrimp penaeus monodon
Authors:   , Soponpong Suthinee 
Published: September 2023

DEAD (Asp-Glu-Ala-Asp) box polypeptide 41 (DDX41), a receptor belonging to the DExD family, was recently identified as an intracellular DNA sensor in vertebrates. Here, we investigated the presence of cytosolic DNA sensing, DDX41 in shrimp Penaeus monodon. By searching for gene involved in DNA sensing cascade within the shrimp P. monodon EST database, 3 cDNA fragments exhibited similarity to DDX41 in various species were identified. Sequences assembly resulted in a complete ORF of Penaeus monodon DDX41 (PmDDX41) which has 1868-bp encoding a putative protein of 620 amino acid residues. The multiple sequence alignment of deduced amino acid sequence of PmDDX41 with other species revealed that there are three conserved domains in the protein: DEADc domain, HELICc domain and Zinc finger domain. PmDDX41 shares 83% similarity with DDX41 in bee and crab. The transcript of PmDDX41 was detected in all tested tissues. Gene expression profile of PmDDX41 in hemocyte of pathogen challenged was analyzed by quantitative RT-PCR. The results showed that the PmDDX41 transcript of White Spot Syndrome Virus (WSSV) challenged shrimp was up-regulated at 6 and 48 hpi when compared to the control shrimp. On the contrary, after challenge with Vibrio harveyi, PmDDX41 mRNA expression was not significantly changed at all time points when compared with the control PBS-injected shrimp. Knockdown of PmDDX41 by dsRNA-mediated gene silencing resulted in a decrease of the mRNA expression level of PmIKKβ, PmIKKɛ, PmPEN3 and PmPEN5 genes. Moreover, PmDDX41-knockdown shrimp exhibited increase in the cumulative mortality after WSSV infection. In addition, PmDDX41 was localized in the cytoplasm of shrimp hemocytes and upon WSSV infection or stimulation the nucleic acid mimics, poly(dA:dT) and poly(I:C), it was found in both the cytoplasm and nucleus of hemocytes. Similar results were observed when PmDDX41 was transfected into human embryonic kidney 293T (HEK293T) cells. Immunoprecipitation further demonstrated that PmDDX41 bound to biotin-labeled poly(dA:dT) but not poly(I:C). The overexpression of shrimp PmDDX41 and mouse stimulator of interferon gene (MmSTING) in HEK293T cells synergistically promoted IFN-β and NF-κB promoter activity via the DEADc domain. Co-immunoprecipitation (Co-IP) also confirmed that there was an interaction between PmDDX41 and STING after stimulation with poly(dA:dT) but not poly(I:C). Our study is the first to demonstrate that PmDDX41 acts as a cytosolic DNA sensor that interacts with STING via its DEADc domain to trigger the IFN-β and NF-κB signaling pathways, thus activating antiviral innate immune responses.
Encapsulation of adzuki bean vigna angularis hydrolysate by alginate-based beads
Authors:   , Sangsukiam Thasanporn 
Published: September 2023

The aims of this research were to study the effect of different cooking conditions on bioactive compounds and biological activities of cooked adzuki beans and cooking water. The different encapsulation methods to entrap adzuki beans hydrolysate were investigated. The release characteristics of different freeze-dried alginate particles were investigated after in vitro simulated gastrointestinal digestion. The results showed that all cooking conditions (60-min boiling, 10-min autoclaving and 60-min autoclaving) caused a significant reduction in crude protein, TCA-soluble peptides, free amino groups, total phenolic content (TPC) and total flavonoid content (TFC) in cooked adzuki beans (p ≤ 0.05). Meanwhile, cooking resulted in a significant increase in TCA-soluble peptides, free amino group content, TPC and TFC in cooking water. The different cooking conditions affected amino acid composition and phenolic compounds. The 10-min autoclaving was the appropriate condition as it could retain more amino acids and release more bound phenolics. The obtained cooked beans and its cooking water contained higher bioactive compounds and showing higher biological activity compared to other conditions. Thus, mixed cooked beans and its cooking water from 10-min autoclaving, substrate for protein hydrolysis, were hydrolyzed with 7% Flavouryzme at 50OC, pH 6.0 for 6 hours. The obtained beans hydrolysate was entrapped in alginate-based via different encapsulation techniques (calcium alginate beads and sodium alginate particles). Freeze-dried adzuki beans hydrolysate was prepared as the control. The sodium alginate particles had a higher encapsulation efficiency of TPC and TCA-soluble peptides compared to calcium alginate beads. Fourier-transform infrared result confirmed the interaction of functional groups between adzuki beans hydrolysate and alginate structure. A remarkable increase in FRAP, ferrous chelating, ORAC value and ACE-I inhibitory after in vitro GI tract digestion were observed in the particles. Whilst beads exhibited higher ABTS scavenging activity after in vitro GI tract digestion compared to particles. DPP-IV inhibitory activity of particles and beads were not significantly different. This might be because encapsulation technique had a significant effect on the entrapped bioactive compounds and controlled-release characteristic of sample.
The prevalence and risk factors of oral soft tissue lesions in kidney transplant recipients
Authors:   , Sirivichayakul Charinrat 
Published: September 2023

Background: Kidney transplantation is considered to be the best treatment for end-stage renal disease patient, and widely accepted it improves quality and length of life. However, the kidney transplant recipient has to receive long-term immunosuppressive therapy in order to prevent kidney transplant rejection, causing the immune function system declined. Oral and dental problems also increase in these patients, which mostly develop as a result of side effects and drug interaction. This study aims to determine the prevalence and risk factors of oral soft tissue lesions in kidney transplant recipients. Methodology: The cross-sectional study was conducted in April to October 2019. Sixty-five kidney transplant recipients and 65 dialysis patients were examined for oral soft tissue lesion, oral hygiene and gingival enlargement. Difference on prevalence of oral soft tissue lesions between groups was tested by Chi-square. For the risk factors, categorical data was presented in frequencies and percentages. Continuous data was presented in means and standard deviations. Univariate and multivariate linear regression was used to assess association between plaque index and different variables. Results: Oral lesions were found in 7 (10.8%) kidney transplant recipients and 6 (9.2%) dialysis patients. Though plaque index in kidney transplant recipients was not significantly different from dialysis group, linear regression model revealed that plaque index was increasing by age (regression coefficient (95% confidence interval) = 0.009 (0.001-0.016); p-value=0.025). Conclusions: Prevalence of oral soft tissue lesions in both groups were not statistically significant different and prevalence in kidney transplant recipients was lower than most of previous studies. Regular dental examination is necessary for early detection, which allows consulting in medications adjustment and stops or relieves progression of oral lesion.
Oral candida colonization in Thai denture wearing elderly with hyposalivation
Authors:   , Buranarom Nada 
Published: September 2023

Objective: To examine relationships between hyposalivation, oral Candida colonization and oral health status in generally healthy elders and evaluate factors that affect salivary flow rates and Candida colonization in a cross-sectional study.

Materials and methods: Fifty-three elderly participants were enrolled and interviewed for medical history, subjective dry mouth symptoms, oral hygiene practice and denture information. Unstimulated and stimulated salivary flow rates, clinical signs of dry mouth, gingival index, tongue coating index and root caries index were recorded. Stimulated saliva samples were cultured on Sabouraud dextrose agar for colony counts and Candida species were identified with chromogenic Candida agar and polymerase chain reaction.

Results: Hyposalivation was associated with higher prevalence of oral Candida colonization (p=0.010; adjusted OR=4.360, 95% confidence interval=1.292-14.717), higher gingival and tongue coating indices (p=0.003 and 0.015, respectively), but not root caries index. These two indices and Candida load were also negatively correlated with unstimulated and stimulated salivary flow rates. Non-albicans Candida species were more frequently isolated in patients who wore dentures (p=0.017).

Conclusion: Hyposalivation is a risk factor for oral Candida colonization and poorer oral health in generally healthy elderly participants. Because hyposalivation could adversely affect oral and systemic health, we suggest that it be carefully monitored in elders.
The study of oro-dental structures and application of dental adhesives in patients affected with osteogenesis imperfecta
Authors:   , Nutchoey Oadcharawadee 
Published: September 2023

Objectives: To investigate genetic mutations, oro-dental features, tooth ultrastructures, and characteristics of total-etch adhesives (Optibond FL; Kerr) and self-etch adhesives (Clearfill SE Bond; Kuraray) applied on the teeth of the patients who affected with syndromic osteogenesis imperfecta and dentiongenesis imperfecta(DGI), compared with the controls. Methods: Three patients were diagnosed with osteogenesis imperfecta and dentinogenesis imperfecta at Faculty of Medicine, Chulalongkorn University. Clinical, radiographic, and laboratory examination were performed. Genetic mutations were analyzed by whole-exome sequencing (WES) and Sanger sequencing. Three teeth were obtained, one tooth from each patient (DGI tooth). Each tooth sample was examined for its color by colorimeter, mineral composition by Micro-CT scan, surface roughness by profilometer, microhardness by Knoop microhardness tester, microscopic morphology by scanning electron microscope (SEM) and histology, and mineral composition by EDX. The microtensile and microshear bond strengths (µTBS and µSBS) of two dental adhesive system, total-etch adhesives (Optibond FL; Kerr) and self-etch adhesives (Clearfill SE Bond; Kuraray), were analyzed compared with the controls. Results: WES identified that the patients with OI and DGI harbored the missense mutations in COL1A2. The DGI teeth were yellowish and dark. The size of dentin and pulp cavity and enamel surface roughness of DGI teeth were diverse. Mineral density of DGI enamel was lower than that of the controls while the mineral density of DGI dentin was variable. Knoop microhardness values of DGI dentin were statistically lower than those of the controls. Correspondingly, DGI enamel also showed reduced microhardness values. Carbon percentage was increased in both DGI enamel and dentin. SEM revealed irregular arrangement and reduced number of dentinal tubules in DGI teeth. Dental adhesive analyses showed that µTBS and µSBS of DGI teeth were lower than those of the controls. Optibond FL showed superior µTBS and µSBS with DGI dentin compared to Clearfil SE bond. Conclusions: This study demonstrates that syndromic DGI teeth of OI patients possessing the missense mutations in COL1A2 exhibited alterations in clinical characteristic, dentin ultrastructure, mineral density, and hardness of dental tissues. These variations could influence the performance of dental adhesives.
Effectiveness of topical anesthesia as an adjuvant to local anesthetic injection during open surgical release of trigger digit: a randomized controlled trial
Authors:   , Laohaprasitiporn Panai 
Published: September 2023

Background: Open trigger digit release in adult is a common hand surgery. Most of the patients considered local anesthetic injection was the most painful step during the procedure. Topical anesthetic drug was not routinely used in the hand surgery. The study on its effectiveness in open trigger digit release has never been studied before.

Objective: To evaluate the effectiveness of topical anesthetic drug in patients who underwent open trigger digit release surgery

Materials and Methods: This study is a randomized controlled trial to compare pain score and patient satisfaction between topical anesthetic cream (5% lidocaine-prilocaine cream) versus placebo cream, applied approximately 90 minutes prior to local anesthetic injection in open trigger digit release surgery. One hundred participants were enrolled and randomly allocated into two groups between May 2019 and February 2020. Visual analog pain score and satisfaction rate were compared.

Results: Most of participants were female with Quinnell grade 2-3. Pain score during needle injection, local anesthetic infiltration, overall pain and satisfaction rate had no statistically significant difference between groups. There was no correlation between duration of topical anesthetic drug application and pain scores. Subgroup analysis did not show significant differences in pain score between genders. No complication was found.

Conclusion: The topical anesthetic drug is ineffective to use at the palmar skin of hand and preoperative application on the surgical site is not necessary for open trigger digit release operation.
Jellyfish sting and first aid knowledge among 6th-9thgrade students in Koh Mak, Koh Kood, and Koh Chang, Trat Province, Thailand
Authors:   , Premmaneesakul Hansa 
Published: September 2023

Background: Injuries from jellyfish are an important public issue. Students are both potential victims and victim-helpers. Objectives: This study aims to investigate the knowledge of jellyfish stings and first aid management in 6th-9th grade students living on three islands in Trat, Thailand. Materials and Methods: A cross-sectional study was conducted by using a questionnaire distributed to all 6th-9th grade students living on these islands. Relationship between total knowledge score and independent variables were assessed by Fisher’s exact test and multiple logistic regression. Results: Of the three islands students from Koh Kood had the highest knowledge (75.27%), followed by students from Koh Chang (59.44%). None of the students from Koh Mak had adequate total knowledge. By class, students from the 9th grade had the highest knowledge (74.47%), followed by 8th, 7th, and 6th grade respectively (63.54%, 62.96%, 53.12%). Female students (67.14%) had a higher total knowledge when compared to males (57.66%). One hundred and fifty-one students (70.89%) who visited the beach 1-2 times/month had the highest total knowledge score, followed by those who visited less than once a month (60.00%), and more than twice a month (55.88%), respectively. The relationship between students’ baseline characteristics and total knowledge score was assessed by multiple logistic regression. Ninth-grade students had 3.2 times higher knowledge compared to 6th grade students (95% CI 1.64, 6.33). Additionally, female students had 1.6 times higher knowledge compared to male students (95% CI 1.00, 2.46). Conclusions: Koh Mak students had the least adequate knowledge on toxic jellyfish stings and first aid; therefore, we should offer more education on this subject to the students. This could be done by including this topic into the science curricula or local subjects. Information on appropriate first aid management of jellyfish via simple infographic material for students can also be provided to adults who are residents and resort operators on all three islands. Everyone should be made aware of the severity associated with toxic jellyfish stings in order to create an effective preventive measure.
Affecting factors on perceived usefulness of area-business continuity management for flood case at industrial areas in Thailand
Authors:   , Meechang Kunruthai 
Published: September 2023

The 2011 Thailand floods widely affected many parts in Thailand such as households, industry and agriculture. Especially, industrial areas were massively damaged by floods. Many factories had to stop their operations. Therefore, it severely impacted economic losses. Area-Business Continuity Management (Area-BCM) is a useful system supporting company mitigate flood effectively. Before the system is implemented, opinion survey of related people in an area is important. Therefore, this research aims to investigate factors affecting Perceived Usefulness (PU) of the Area-BCM. The model is proposed based on Technology Acceptance Model and flood mitigation behavior. The analysis results categorized the respondents into four groups following their experience with floods. It is found that subjective norm significantly affect to PU. In addition, flood hazards knowledge significantly positively affect to PU in experienced group. On the other hand, worry about flooding indirectly affect to PU through subjective norm. Moreover, government is found to be the most trusted source by respondents in receiving disaster information. They also mentioned warning information should be provided. Furthermore, the findings are expected to be used as a guideline for developing a prototype of the Area-BCM system.