bluuebunny/crossref_metadata_2025_split huggingface dataset:
Comparative evaluation of post-operative pain & analgesic intake after root canal treatment using three bioceramic sealers- An In-vivo study
Authors: Kour Sukhbir , Kaul Rudra , Choudhary Ashish , Malik Azhar , Kumar Ajay , Dhani Rachna 
Published: April 2023

To evaluate the occurrence and intensity of post-operative pain & number of analgesic tablets intake after single visit endodontic treatment using three different Bioceramic sealers.45 single rooted teeth diagnosed with asymptomatic irreversible pulpits requiring endodontic treatment were randomly assigned to three groups (n=15) according to sealer used during obturation. To avoid bias in post-operative pain perception, each sealer is evaluated in three different single rooted teeth of the same patient. The different groups are Group 1-MTA-Fillapex, Group –II Ceraseal RCS, Group-III BioRoot RCS. Single visit Endodontic treatment was performed and teeth were obturated using cold lateral compaction and different root canal sealers with no radiographic material extrusion beyond the apex. Severity of post obturation pain was assessed by Visual Analog Scale (VAS) with score ranging from 0 to 10 at 6hrs, 12hrs, 24hrs and 48hrs intervals. The need for analgesic (Tab. Aceclofenac 100 mg) intake was also recorded. Differences in incidence of postoperative pain were analyzed using the Kruskal Wallis test followed by Mann Whitneys Post Hoc to compare the mean VAS score & number of analgesics taken between three groups at different time intervals (p&#60;0.05).MTA Fillapex shows statistically significant difference in mean VAS score (more pain) at 6hrs, 12hrs (p value &#60; 0.001) & at 24hrs (p&#60;0.005) than Ceraseal RCS and BioRoot RCS. There is statistically significant difference in the no. of analgesic intake for MTA Fillapex i.e., more tablets taken by the patient to relive pain (p&#60;0.001) as compared to Ceraseal RCS and BioRoot RCS. The decrease in pain was statistically significant for each time intervals from 6hrs to 48hrs for all the three groups.The teeth of the patients where MTA Fillapex sealer was used as a sealer in the obturation showed significantly more pain at 6hrs & 12hrs and needed to take more analgesics than teeth obturated with other two sealers. This probably could be due to leaching out of resin constituents from MTA Fillapex.
THE RELATIONSHIP OF PARENTS KNOWLEDGE AND SOCIOECONOMIC STATUS BASED ON PERFORMANCE TREATMENT INDEX
Authors: Rizkia Namira Aulia , Hatta Isnur , Dwi Kurniawan Fajar Kusuma 
Published: April 2023

ABSTRACTBackground: Dental and oral health problems are related to individual motivation to maintain oral and dental health. It is measured by the Performance Treatment Index (PTI). The average percentage of teeth decay, cavities and pain in Kotabaru regency is greater than the average percentage in South Kalimantan, which is 55.19% and the average percentage of filling in Kotabaru Regency is 2.72%. The level of knowledge and socioeconomic status affect individual motivation to maintain dental and oral health. Purpose: To analyze the relationship between knowledge of oral health and the socioeconomic status of parents with the Performance Treatment Index in students of SMPN 1 Kotabaru. Methods: This research is an analytic observational with a cross sectional approach. The sampling technique by simple random sampling with total 34 person. Results: The level of knowledge of parents on dental and oral health is mostly in the good category as many as 23 person (68%). The level of socioeconomic status of parents is mostly in the middle category with 21 person (62%). The level of Performance Treatment Index for SMPN 1 Kotabaru students is mostly in the category above the national standard with 15 person (44%). Spearman test results showed the relationship between parental knowledge and PTI with p value = 0.00 and a correlation coefficient of 0.719. The relationship between parents' socioeconomic status and PTI with p value = 0.00 and a correlation coefficient of 0.630. Conclusion: Based on on the research it can be concluded that there is a significant relationship between knowledge and socioeconomic status of parents with the Performance Treatment Index in students of SMPN 1 Kotabaru with a strong positive correlation. Keywords: Knowledge, Performance Treatment Index, Socioeconomic
THE EFFECT OF KASTURI LEAF EXTRACT ON THE HEMOGLOBIN LEVEL EXPOSED TO PERIAPICAL RADIOGRAPHY X-Rays
Authors: Damayanti Firda , Aspriyanto Didit , Putri Utami Juliyatin 
Published: April 2023

ABSTRACTBackground: Periapical radiographic x-ray exposure can damage living cells, one of which is erythrocytes which contain hemoglobin by forming free radicals. Antioxidant compounds are needed to counteract the effects of these free radicals. One of the natural antioxidant compounds is kasturi leaves which contain flavonoids, triterpenoids and tannins. Purpose: To analyze the effect of kasturi leaf extract on hemoglobin levels of male mice exposed to periapical x-ray radiography. Methods: This study used a true experimental method with a post test only design with a control group design. The samples used were 24 male mice aged 3-4 months which weighed 20-25 grams. Mice were divided into 4 groups, each group consisted of 6 mice. Group P1 was a group exposed to radiation 1 times a dose of 1 mGy with a dose of 0,2 ml of kasturi leaf extract, group P2 was a group exposed to radiation 7 times a dose of 1 mGy with a dose of 0,2 ml of kasturi leaf extract, Group P3 was a group exposed to radiation 10 times at a dose of 1 mGy with a dose of 0,2 ml of kasturi leaf extract, and group P4 was a group given the extract without radiation exposure. Results: The results showed hemoglobin levels in the P1 group = 7.2833 g/dL, the P2 group = 7.4800 g/dL, the P3 group = 12.7800 g/dL, and P4 = 12.4200 g/dL. Conclusion: The results showed that there was an effect of kasturi leaf extract on the hemoglobin levels of male mice exposed to periapical x-ray radiography. The results of the Mann Whitney test showed that the results of the P1 and P2 groups were significant to P3 and P4. Keywords: Antioxidant, kasturi leaf extract, periapical radiography
Diagnostic and therapeutic value of EVs in Lungs Diseases and Inflammation
Authors: Khawar Muhammad Babar , Afzal Ali , Habiba Ume , Hamid Syeda Eisha , Abbasi Muddasir Hassan , Rafiq Mussarat , Sheikh Nadeem , Abaidullah Rimsha , Asif Zoya , Saeed Tahaa 
Published: April 2023

Abstract
Extracellular Vesicles (EVs) are membrane-derived messengers which have been playing an important role in the inflammation and pathogenesis of lung diseases. EVs contain varieties of DNA, RNA, and membrane receptors through which they work as a delivery system for bioactive molecules as well as intracellular communicators. EV signaling mediates tumor progression and metastasis. EVs are linked with many diseases and perform a diagnostic role in lung injury and inflammation so are used to diagnose the severity of diseases. EVs containing a variety of biomolecules communicate with the recipient cells during pathophysiological mechanisms thereby acquiring the attention of clinicians toward the diagnostic and therapeutic potential of EVs in different lung diseases. In this review, we summarise the role of EVs in inflammation with an emphasis on their potential as a novel candidate in the diagnostics and therapeutics of chronic obstructive pulmonary disease (COPD), asthma, and sarcoidosis.
Genetic Diversity and Population Structure Analysis of Bread Wheat (Triticum aestivum L.) Germplasms as Revealed by ISSR markers
Authors: Atsbeha Genet - , Tesfaye Kassahun - , Mekonnen Tilahun - , Haileselassie Teklehaimanot - , Kebede Mulugeta - 
Published: April 2023

Abstract

Wheat is one of the major food crop in Ethiopia and elsewhere in the world. Genetic diversity provides breeders chance to create new, improved varieties with desirable traits. In this study genetic diversity and population structure of 180 bread wheat germplasms representing eight populations were analyzed using ten Inter Simples Sequence Repeat (ISSR) marker. Based on diversity indices all the ISSR markers were polymorphic and highly informative to describe genetic diversity and population structure of the crop. High within-populations genetic diversity with a mean of alleles of 1.47, effective alleles of 1.33, Shannon’s Information index of 0.29, Nei's gene diversity value of 0.19, and percent of polymorphic loci (PPL) of 80.5% were reviled. Among the populations, international Septoria observation nursery (ISEPTON) showed relatively highest observed number of alleles (1.47), effective number of alleles (1.33), Shannon’s Information index (0.4) and Nei's gene diversity (0.19). Thus, ISEPTON lines could be good source of useful alleles to be used in wheat improvement programs. Analysis of molecular variance (AMOVA) revealed that 95% of the total genetic variation resides within populations, and 5% was accounted for the among populations variation. Clustering using Unweighted pair group method with arithmetic mean (UPGMA), Principal coordinates analysis (PCoA) and population structure analysis did not group the wheat populations into genetically distinct clusters following their breeding objectives. Adequate polymorphism and reproducible fingerprinting profiles were explored form the genetic structure analysis of the wheat germplasms. The information obtained could be useful in future bread wheat breeding programs, and also its conservation.
Outcomes of Interferon-free Direct Acting Antivirals in Patients with HCV-related Decompensated Cirrhosis: A Systematic Review and Meta-analysis
Authors: Jongraksak Tanawat , Chuncharunee Alan , Intaraprasong Pongphob , Tansawet Amarit , Thakkinstian Ammarin , Sobhonslidsuk Abhasnee 
Published: April 2023

Abstract
Direct-acting antiviral (DAA) is an effective treatment for chronic hepatitis C. However, the treatment benefit on overall and hepatocellular carcinoma-free survival remains unanswered. This meta-analysis aimed to assess overall survival (OS), HCC-free survival and the Model for End-stage Liver Disease score (MELD) improvement. Relevant studies were identified by searching MEDLINE, SCOPUS and CENTRAL. OS and HCC-free survival probabilities and time data were extracted from Kaplan-Meier curves. A one-stage meta-analysis using parametric Weibull regression was applied to estimate relative treatment effects of DAA versus no-DAA. Eight cohorts with 3430 participants (2603 cases in DAA and 1999 cases in no-DAA groups) were included. The OS probabilities at 12- and 24-months were 95% and 90% for the DAA group, comparing with 89% and 80% in the no-DAA group. The hazard ratios (HR) was 0.48 (95% CI: 0.39, 0.60; p<0.001). The HCC-free survival probabilities at 12- and 24-months were 96% and 90% in the former and 94% and 85% in the later. The HR was 0.72 (95% CI: 0.52, 1.00; p = <0.001). The mean MELD score difference was -7.75 (95% CI: -14.52, -0.98; p = 0.02). DAA treatment in decompensated HCV cirrhosis patients prolongs OS, improves MELD score and may reduce HCC occurrence.
Trial Registration: PROSPERO (ID:CRD42022316276).
Plant Polyploidy: Origin, Induction, Phenotypic Change Mechanisms, Population Establishment, and Distribution
Authors: Yang Aiyiwei , Chen Xiaoyu , Chen Su 
Published: April 2023

Abstract
Polyploidy refers to individuals that contain two or more heritable sets of chromosomes. It is an important feature of chromosome evolution in eukaryotes and is very common in plants in nature. Polyploidy forms in nature in many ways. Researchers have also mastered a variety of polyploid induction and detection methods. In the process of plant polyploidization, a variety of genetic and epigenetic changes occur. This reduces the redundancy of polyploidy genes and changes their traits, promoting the long-term evolution and diversification of polyploidy. Polyploidy has many strategies to overcome minority cytotype exclusion (MCE) during population establishment to coexist stably with diploid parents. Due to differences in traits, polyploidy changes its interaction with the environment, and there is a certain regular distribution in natural and urban ecosystems. Polyploids have been used in several fields, including forestry, and agriculture. In this review, we summarized the natural formation of polyploidy artificial polyploids induction, detection of polyploidy, and mechanism of phenotypic differences between polyploids and diploidy.
Identification and pathogenicity analysis of Fusarium spp. on peach in China
Authors: Dong Jingping , Shi Hengsong , Wu Yu , Yang Lina , Zhu Feng , Ji Zhaolin 
Published: April 2023

Abstract
Background Vascular diseases with symptoms of vascular browning and branch dying seriously impact peach yield worldwide. This study aimed to identify and analyze the pathogenicity of Fusarium spp. isolates on the diseased branches of peach trees obtained from several peach-producing areas of China.Results We obtained and confirmed nine Fusarium isolates based on the morphological and molecular characteristics and phylogenetic relationships using a combination of rDNA–internal transcribed spacer, elongation factor (EF)-1α, and mitochondrial small subunit gene sequences. GJH-Z1, GJH-6, and GJH-1 were identified as F. avenaceum strains; GG-2020-1, HYR-Z3, ZLZT-6, and HH-2020-G2 were identified as F. solani strains; SYGZ-1 was identified as F. equiseti strain; and HYTZ-4 was identified as F. lateritium strain. A comparison of the pathogenicity of the nine isolates on peach branches displayed that the F. equiseti (SYGZ-1) strain showed a higher level of pathogenicity compared with other strains using the acupuncture method, and F. solani (HYR-Z3 and ZLZT-6), F. equiseti (SYGZ-1), and F. lateritium (HYTZ-4) strains showed a higher level of pathogenicity using the impregnation method.Conclusions This study was novel in reporting that Fusarium spp. caused vascular bundle browning of peach branches in China. The findings might help better elucidate the basis of pathogenicity in peaches in the future.
Effect of thermal manipulation on the biological and mechanical characteristics of horizontal platelet rich fibrin membranes
Authors: Wu Qian , Yu Shimin , Wang Yulan , Zhang Xiaoxin X. 
Published: April 2023

Abstract
Purpose Regardless of application scenarios, proper mechanical characteristics and degradation property is the prerequisite for horizontal platelet rich fibrin (H-PRF) to manifest its ability. Among the methods used to modify PRF, thermal manipulation is promising as it is easy to handle without adding extra additives. Yet there is no consensus on optimal temperature treatment. The present study aimed to investigate the effect of heating on the biological and mechanical characteristics and explore the most suitable heating temperature for H-PRF thermal treatment.Methods We employed a series of temperature gradients, room temperature, 50℃, 75℃, 90℃, 105℃. The microstructure and the mechanical properties were recorded by Scanning Electron Microscope (SEM) and tensile strength tests respectively. The degradation rate of H-PRF membranes was examined by digestion assay with plasmin and trypsin. The viability of cells within H-PRF membranes and the proliferation of osteoblasts cultured with extracts from different H-PRF groups was evaluated using CCK-8 assays.Results Compared with the nonheated group, over-heated manipulation beyond 90℃ can significantly prolong the degradation properties up to 3 to 4 weeks and enhance the mass stress of H-PRF membranes. A high temperature treatment of 105℃ accompanied with the cell activity beneath H-PRF reduced more than half and thus the biological effect to human osteoblasts (hFOBs) also reduced dramatically.Conclusions High thermal manipulation can prolong the degradation properties and enhance the mechanical properties of PRF membranes while accompanied by the loss of biological effect.
Llamas (Llama glama) enhance novel proglacial ecosystem development: an experimental approach in the Cordillera Blanca, Peru
Authors: Zimmer Anais , Beach Timothy , Regalado Sebastián Riva , Aliaga Jean Salcedo , Encarnación Rolando Cruz , Anthelme Fabien 
Published: April 2023

Abstract

Worldwide, mountain glaciers are shrinking rapidly and projected to disappear largely by 2100. Consequently, large areas are becoming available for novel alpine ecosystems. These harsh environments, however, slow down primary succession. In this study with a local community, we conducted an inclusion experiment to investigate if and howLlama glamainfluences soils and vegetation primary succession following glacial retreat. At the foot of the Uruashraju glacier in the Cordillera Blanca, Peru (~ 4680 m.a.s.l.), we established four llama inclusion plots and four control plots that we studied from 2019 to 2022, 24–40 years after glacial retreat. After three years, the llama plots had significantly increased soil organic carbon and soil nitrogen. In the llama plots, we found a large, significant increase in vascular plant cover (+ 57%) between the second and third years of experimentation, and we identified four new species that were not present in 2019. Our results suggest thatLlama glama, through their latrine behavior and role as a seed disperser, enhances the primary succession and novel ecosystem formation in recently deglaciated landscapes. Our study provides scientific support that rewilding of native Andean camelid may favor adaptation to glacier retreat and strengthen the conservation and management of novel proglacial ecosystems.
Targeted Deep Brain Stimulation of the Motor Thalamus Facilitates Voluntary Motor Control After Cortico-spinal Tract Lesions
Authors: Ho Jonathan , Grigsby Erinn , Damiani Arianna , Liang Lucy , Balaguer Josep-Maria , Kallakuri Sridula , Barrios-Martinez Jessica , Karapetyan Vahagn , Fields Daryl , Gerszten Peter , Hitchens T. , Constantine Theodora , Adams Gregory , Crammond Donald , Capogrosso Marco , Gonzalez-Martinez Jorge , Pirondini Elvira 
Published: April 2023

Abstract
Cerebral white matter tract lesions prevent cortico-spinal descending inputs from effectively activating spinal motoneurons, leading to untreatable muscle paralysis. However, in most cases the damage to cortico-spinal axons is incomplete and the spared connections could be potentiated by neurotechnologies to restore motor function. Here we hypothesized that, by engaging direct excitatory connections to cortico-spinal motoneurons, deep brain stimulation (DBS) of the motor thalamus could facilitate activation of spared cortico-spinal fibers improving muscle activation of the paretic limb. We first identified, in monkeys, optimal stimulation targets and parameters that enhanced motor evoked potentials to arm, hand, and face muscles, as well as grip forces. This potentiation persisted after cerebral white matter lesions. We then translated these results to human subjects by identifying the corresponding optimal thalamic targets (VIM/VOP nuclei) and replicated the results obtained in monkeys. Finally, we designed a DBS protocol that immediately improved voluntary grip force control in a patient with a chronic traumatic brain injury. Our results suggest that targeted DBS of the motor thalamus may become an effective therapy for motor paralysis.
Effect of a supersaturated calcium-phosphate rinse on oral mucositis in patients undergoing hematopoietic stem cell transplantation (HSCT): a systematic review and meta- analysis
Authors: de Vries J.A. , Vos M.H.G.F. , Etten-Jamaludin F.S. , Maaskant J.M. 
Published: April 2023

Abstract
Background Oral mucositis (OM) is a common complication of hematopoietic stem cell transplantation (HSCT). OM is very painful, influences nutritional intake and impairs patients’ quality of life (QoL). Although several studies have investigated the potential of supersaturated calcium-phosphate rinse (SCPR) in reducing the incidence, duration and severity of OM in patients undergoing HSCT, conclusive results have not yet been found. Therefore, the aim of this systematic review and meta-analysis is to comprehensively evaluate whether SCPR is effective in reducing the incidence, duration and severity of HSCT-induced OM.Methods Five electronic databases were searched: PubMed, EMBASE, the Cochrane Library, CINAHL and Google Scholar up to August 2021. Both randomized controlled trials (RCTs) and non-randomized studies of interventions (NRISs) were included. Two reviewers independently screened titles, abstracts and full texts for study inclusion, and extracted data from the included studies. We used the RoB 2 and ROBINS-I to critically appraise the included studies, and GRADE to assess the quality of evidence.Results Out of 1008 publications, 8 RCTs and 3 NRSIs with in total 830 participants, were included. Meta-analyses showed that SCPR did not decrease the incidence of OM: 3 studies (RR, 0.93; 95% CI, 0.80–1.08), moderate severe OM: 2 studies (RR, 1.16; 95% CI, 0.85–1.59) and severe OM: 2 studies (RR, 0.66; 95% CI, 0.27–1.64). Meta-analysis was not possible for the duration of OM due to heterogeneity between the studies.The overall quality of evidence was moderate for the incidence of OM and low for the duration and severity of OM.Conclusions Based on the available evidence, SCPR does not seem effective in reducing the incidence, duration and severity of OM in patients undergoing HSCT. Further large-scale studies with more rigorous methodology are required to enhance the quality of evidence.
Identification of Uromodulin Deposition in the Stroma of Perinephric Fibromyxoid Nephrogenic Adenoma by Mass Spectrometry
Authors: Yoshimura Kaori , Ito Yukinobu , Suzuki Mina , Horie Masafumi , Nishiuchi Takumi , Shintani-Domoto Yukako , Shigehara Kazuyoshi , Ohshima Hiroko , Ohshima Masanobu , Goto Akiteru , Nojima Takayuki , Tsuzuki Toyonori , Mizokami Atsushi , Ikeda Hiroko , Maeda Daichi 
Published: April 2023

Abstract
Background Nephrogenic adenoma (NA) is an epithelial lesion that usually occurs in the mucosa of the urinary tract. Rare cases of deep infiltrative or perinephric lesions have also been reported. Recently, NA with characteristic fibromyxoid stroma (fibromyxoid NA) has been proposed as a distinct variant. Although shedding of distal renal tubular cells due to urinary tract disorder has been postulated as the cause of NA in general, the mechanism underlying extraurinary presentation of NA and fibromyxoid stromal change in fibromyxoid NA remains unknown.Case presentation: An 82-year-old man had distal ureteral carcinoma and underwent total nephroureterectomy. The patient had no prior history of urinary tract injury or radiation. Periodic acid-Schiff staining-positive eosinophilic deposits were seen in the periureteral and perinephric regions proximal to the ureteral carcinoma. The eosinophilic structureless deposits in the stroma of fibromyxoid NA were microdissected and subjected to liquid chromatography/mass spectrometry (LC/MS). The analysis revealed the presence of a substantial amount of uromodulin (Tamm–Horsfall protein).The presence of urinary content in the stroma of perinephric fibromyxoid NA suggests that urinary tract failure and engraftment of renal tubular epithelial cells directly cause the lesion.Conclusions In conclusion, we reported a rare case of perinephric fibromyxoid NA with uromodulin deposits. In this study, proteomics analysis suggested the involvement of urinary material leakage. Mass spectrometry may be most effective when performed in lesions showing a characteristic morphology, especially in the stroma.