bluuebunny/crossref_metadata_2025_split huggingface dataset:
Effects of oral β‐cryptoxanthin administration on the transcriptomes of peripheral neutrophil and liver tissue using microarray analysis in post‐weaned Holstein calves
Authors: Kimura Atsushi , Kim Yo‐Han , Hashizume Kazuyoshi , Ito Akira , Mukai Katsuyuki , Kizaki Keiichiro , Sato Shigeru 
Published: March 2023

AbstractWe investigated the effects of oral administration of β‐cryptoxanthin (β‐CRX), a precursor of vitamin A synthesis, on the transcriptomes of peripheral neutrophils and liver tissue in post‐weaned Holstein calves with immature immunity. A single oral administration of β‐CRX (0.2 mg/kg body weight) was performed in eight Holstein calves (4.0 ± 0.8 months of age; 117 ± 10 kg) on Day 0. Peripheral neutrophils (n = 4) and liver tissue (n = 4) were collected on Days 0 and 7. Neutrophils were isolated by density gradient centrifugation and treated with the TRIzol reagent. mRNA expression profiles were examined by microarray and differentially expressed genes were investigated using the Ingenuity Pathway Analysis software. The differentially expressed candidate genes identified in neutrophils (COL3A1, DCN, and CCL2) and liver tissue (ACTA1) were involved in enhanced bacterial killing and maintenance of cellular homoeostasis respectively. The changes in the expression of six of the eight common genes encoding enzymes (ADH5 and SQLE) and transcription regulators (RARRES1, COBLL1, RTKN, and HES1) were in the same direction in neutrophils and liver tissue. ADH5 and SQLE are involved in the maintenance of cellular homoeostasis by increasing the availability of substrates, and RARRES1, COBLL1, RTKN, and HES1 are associated with the suppression of apoptosis and carcinogenesis. An in silico analysis revealed that MYC, which is related to the regulation of cellular differentiation and apoptosis, was the most significant upstream regulator in neutrophils and liver tissue. Transcription regulators such as CDKN2A (cell growth suppressor) and SP1 (cell apoptosis enhancer) were significantly inhibited and activated, respectively, in neutrophils and liver tissue. These results suggest that oral administration of β‐CRX promotes the expression of candidate genes related to bactericidal ability and regulation of cellular processes in peripheral neutrophils and liver cells in response to the immune‐enhancing function of β‐CRX in post‐weaned Holstein calves.
The pharmacokinetics of single oral dose extended‐release topiramate and adverse effects after multi‐dose administration in healthy cats
Authors: Graham Lindsey T. , Foss Kari D. , Reinhart Jennifer M. , Smith Kathryn M. , Hague Devon W. , Li Zhong 
Published: March 2023

AbstractCurrent treatment options for feline epilepsy are limited to medications that require administration of multiple doses per day or administration of a capsule or large tablet. Expanding the current treatment options could improve patient and owner compliance and optimize seizure control. Topiramate has been used sparingly in veterinary medicine, and limited pharmacokinetic studies have focused on immediate release formulations in dogs. If effective and safe, topiramate extended‐release (XR) could broaden the current treatment options for feline epilepsy. The aims of this two‐phase study were to establish single‐dose pharmacokinetics for topiramate XR in cats, identify a dosing regimen that maintains steady‐state plasma drug concentrations within a reference range extrapolated from human medicine (5–20 μg/mL), and evaluate the safety of topiramate XR in cats following multidose administration. Topiramate XR administered orally at 10 mg/kg once daily for 30 days was sufficient to achieve the desired concentrations in all cats. While no clinically apparent adverse effects were observed, four out of eight cats developed subclinical anemia, calling into question the safety of topiramate XR with chronic administration. Further studies are necessary to better understand the potential adverse effects and overall efficacy of topiramate XR for the treatment of feline epilepsy.
Relationship between serum allergen‐specific immunoglobulin E and threshold dose in an oral food challenge
Authors: Yanagida Noriyuki , Sato Sakura , Nagakura Ken‐ichi , Takahashi Kyohei , Fusayasu Naoko , Miura Yoko , Itonaga Takaaki , Ogura Kiyotake , Ebisawa Motohiro 
Published: March 2023

AbstractBackgroundSeveral studies have reported threshold doses for food allergens. However, evidence regarding potential risk factors for low threshold doses is limited. Moreover, the relationship between threshold dose and specific immunoglobulin E (sIgE) levels to causative foods remains unclear. This study examined the relationship and the risk factors for a low threshold dose.MethodsWe recruited children with food allergies and examined the risk factors for a positive oral food challenge (OFC) with a low threshold dose and anaphylaxis.ResultsWe evaluated 2501 children with food allergies (1667 [67%] boys; median age, 4.9 years) to eggs (n = 1096), milk (n = 671), wheat (n = 370), peanuts (n = 258), walnuts (n = 65), and cashews (n = 41). Of these patients, 234 (9%) reacted to ≤30 mg protein of causative foods and 620 (25%) reacted to ≤100 mg protein of causative foods. The sIgE level to causative foods was a significant independent factor for positive OFCs with a threshold dose of ≤30 mg for milk, wheat, and peanuts; ≤ 100 mg for eggs, milk, wheat, peanuts, and cashews; and anaphylaxis from eggs, milk, wheat, peanuts, and walnuts. High sIgE levels to causative foods were associated with a lower threshold dose of the OFC and anaphylaxis during the OFC.ConclusionsApproximately 9% of patients reacted to ≤30 mg protein of causative foods. The potential risks of anaphylaxis should be considered during OFCs for patients with elevated sIgE levels.
Anxiety in children: A review on how to address it in the perioperative setting post‐pandemic
Authors: Challa Chaitanya , Geng‐Ramos Giuliana , Gray Laura , Orshan Taytum , Thackeray Laura , Gupta Pooja 
Published: March 2023

AbstractIn this paper, we review the psychological burden of SARS‐CoV‐2 on children and how health care workers can play a role in mitigating its mental health impact during anesthetic procedures. We evaluate the societal changes that have affected children over 2 years of the pandemic and the subsequent soaring rates of anxiety and depression reported. Unfortunately, the perioperative setting is a stressful experience at baseline and the addition of COVID‐19 has only exacerbated the situation. Anxiety and depression are often linked to maladaptive behavior post‐surgery, including increased rates of emergence delirium. Providers can utilize techniques based on developmental milestones, Certified Child Life Specialists, parental presence during induction, and medications to reduce anxiety. As health care workers, we need to recognize and address these concerns as untreated mental health issues can leave long‐term consequences for children.
Changes in parents' attitudes towards childhood vaccines during COVID‐19 pandemic
Authors: Duran Sedef , Duran Rıdvan , Acunaş Betül , Şahin Erkan Melih 
Published: March 2023

AbstractBackground: Concerns about the safety and adverse reactions of rapidly‐developed vaccines against COVID‐19 contributed to parents' vaccine hesitancy and this situation created an opportunity for anti‐vaccine campaigners. The aim of this study was to examine the changes in parents' attitudes towards childhood vaccines during COVID‐19 pandemic.Methods: In this cross‐sectional study, parents of children who applied to the outpatient clinic of pediatric department of Trakya University Hospital, Edirne, Türkiye, between August 2020 and February 2021 were recruited into two study groups according to COVID‐19 peak time in Türkiye. Group 1 included parents of children who applied after first peak of the COVID‐19 pandemic and Group 2 included parents of children who applied after second peak. The World Health Organization 10‐item Vaccine Hesitancy Scale (WHO‐VHS) was applied to each group.Results: A total of 610 parents agreed to participate in the study. Group 1 and 2 consisted of 160 and 450 parents, respectively. While the number of parents who were hesitant about childhood vaccines was 17 (10.6%) in Group 1, it was 90 (20%) in Group 2. A statistically significant difference was found between the two groups (p = 0.008). The (WHO‐VHS) mean ± SD score was found to be higher in Group 2 (23.7 ± 6.9) than Group 1 (21.3 ± 7.3) (p < 0.001). The WHO‐VHS‐ mean ± SD scores of parents who experienced COVID‐19 infection themselves or their family or acquaintances were significantly lower than those who did not (20.0 ± 6.5 vs. 24.7 ± 6.9) (p < 0.001).Conclusions: The hesitant attitudes towards childhood and COVID‐19 vaccines were low in parents who directly or indirectly experienced COVID‐19 or were worried about the devastating effects of this disease. However, it was shown that as the COVID‐19 pandemic progressed, parents' hesitations towards childhood vaccines increased.
On List's compatibilist libertarianism
Authors: Moore Dwayne , Ugljesic Sara 
Published: March 2023

AbstractChristian List has recently presented a compatibilist libertarian solution to the free will and determinism problem. He proposes the admixture of libertarianism, which endorses agential alternative possibilities, with physical determinism, which endorses the necessity of physical effects. In this paper, we argue that List's innovative proposal ultimately fails.
From Africa to the world—The journey of Nile tilapia
Authors: El‐Sayed Abdel‐Fattah M. , Fitzsimmons Kevin 
Published: March 2023

AbstractDespite the fact that Nile tilapia (Oreochromis niloticus) (Linnaeus, 1758) are African freshwater fish, they have been introduced into many countries inside and outside Africa. The journey of Nile tilapia started during the second half of the 20th Century, especially in Southeast Asia and the Americas, mainly for aquaculture and fisheries enhancement. Most of these introductions became well established in aquaculture. As a result, aquaculture of Nile tilapia has been steadily expanding in many countries. These fish play an important role in the livelihoods of local societies on different continents. This review summarizes the current knowledge on global Nile tilapia introductions and sheds light on their social and economic contributions to the countries into which they have been introduced, as well as their share in the global fish market. The ecological impacts of tilapia introductions have also been addressed. The success stories of the major Nile tilapia producers were highlighted so that the lessons learned from their experiences could be transferred to other countries.
A global review of problematic and pathogenic parasites of farmed tilapia
Authors: Shinn Andrew P. , Avenant‐Oldewage Annemarie , Bondad‐Reantaso Melba G. , Cruz‐Laufer Armando J. , García‐Vásquez Adriana , Hernández‐Orts Jesús S. , Kuchta Roman , Longshaw Matt , Metselaar Matthijs , Pariselle Antoine , Pérez‐Ponce de León Gerardo , Pradhan Pravata Kumar , Rubio‐Godoy Miguel , Sood Neeraj , Vanhove Maarten P. M. , Deveney Marty R. 
Published: March 2023

AbstractOver the past 80 years, tilapia have been translocated globally for aquaculture; active production is recorded in >124 countries. Of 7 million tonnes of tilapia produced in aquaculture, 79% is from 79 countries outside the natural range of tilapia. Capture fisheries account for a further 723,627 tonnes of tilapia, and >47% of this is landed from established invasive populations outside Africa. Tilapias host a rich fauna of parasites, many of which have been translocated with their hosts. This review summarises >2500 host–parasite records from 73+ countries and >820 recorded tilapia translocations (provided in the supplementary materials). This work focuses on the notable pathogens that threaten the health of cultured populations of tilapia, providing a description of their pathology and includes species that also have substantial impacts on wild tilapia populations, where relevant. For each major parasite taxonomic group, we highlight which parasites have been translocated or have been acquired from the new environments into which tilapia have been introduced, together with remarks on standard treatment approaches and research on them and their management and control. Regarding the theme ‘Tilapia health:quo vadis?’, Africa has enormous potential for aquaculture growth, but substantial knowledge gaps about tilapia parasites in many African states remain, which creates associated production and biosecurity risks. For each parasitic group, therefore, the risks of parasite translocation to new regions as tilapia aquaculture industries expand are highlighted.
Bacterial diseases of tilapia, their zoonotic potential and risk of antimicrobial resistance
Authors: Haenen Olga L. M. , Dong Ha Thanh , Hoai Truong Dinh , Crumlish Margaret , Karunasagar Iddya , Barkham Timothy , Chen Swaine L. , Zadoks Ruth , Kiermeier Andreas , Wang Bing , Gamarro Esther Garrido , Takeuchi Masami , Azmai Mohammad Noor Amal , Fouz Belén , Pakingking Rolando , Wei Zeng Wei , Bondad‐Reantaso Melba G. 
Published: March 2023

AbstractTilapia culture is an important source of income and nutrition to many rural families. Since 2000, the production of tilapia increased and reached domestic and global markets. Major farmed species is Nile tilapia (Oreochromis niloticus), in earthen ponds and cage cultures. Intensification contributed to global tilapia disease outbreaks, with bacterial infections causing mortalities and morbidities, threatening sustainable production. At tilapia farms, high nutrient concentrations, water temperature and fish densities enhance bacterial growth including virulent bacterial clones and potential zoonotic bacteria. Global warming favours this. This review respectively provides a comprehensive overview of the most common and emerging bacterial pathogens, diseases, clinical presentations and diagnostics of tilapia, including bacteria and diseases with zoonotic potential. First, common bacterial disease outbreaks, including streptococcosis, motileAeromonassepticaemia, francisellosis, columnaris disease and vibriosis are described. Then, information on emerging bacterial infections of concern for tilapia, like edwardsiellosis throughEdwardsiella ictaluriandE.tarda, as well asAeromonas schubertiiis provided. Reports of infectious bacterial tilapia disease outbreaks from other bacteria, includingLactococcus garvieae,Aerococcus viridans,Pseudomonasspp.,Mycobacterium marinumandChlamydiaspp., and others are reviewed. Furthermore, bacteria with zoonotic potential, likeStreptococcus agalactiaeST283,S.iniae,Aeromonassp.,E.tarda,Vibrio vulnificuspathovar (pv)piscisandM.marinumare included in the review, to provide the most current overview of the disease risks affecting production and post‐harvest stages. Additionally, the status and risks of antimicrobial resistance in bacteria from tilapia and other cultured fish through imprudent use of antibiotics, and its future at a global level are provided.
Improving tilapia biosecurity through a value chain approach
Authors: MacKinnon Brett , Debnath Partho Pratim , Bondad‐Reantaso Melba G. , Fridman Sophie , Bin Hao , Nekouei Omid 
Published: March 2023

AbstractTilapia aquaculture is a major source of animal protein, with global production reaching over 6 million tonnes in 2020. The rapid growth of the tilapia sector has led to a number of emerging disease threats and subsequent production losses. Risk analysis can provide a targeted approach for improving biosecurity in the tilapia sector. The aim of this work was to describe the tilapia value chain and review the important infectious agents of tilapia that may affect the different points along the value chain; such points include input and service suppliers, producers (i.e., hatcheries, nurseries and grow‐out farms), and processors, traders and marketers. We then describe how risk analysis can be used to identify critical controls points along the value chain and describe potential risk mitigation measures that may be implemented at those points. The control of diseases of tilapia requires a multi‐faceted approach, with risk‐based control measures chosen based on their feasibility, effectiveness and sustainability. The Progressive Management Pathway for Improving Aquaculture Biosecurity, as a risk‐based, collaborative and progressive management approach combined with the systematic preventive principles of Hazard Analysis Critical Control Point, offers a strategic and practical way of improving biosecurity in the tilapia value chain.
Chronic adrenergic stress and generation of myeloid‐derived suppressor cells: Implications for cancer immunotherapy in dogs
Authors: Ammons Dylan T. , MacDonald Cameron R. , Chow Lyndah , Repasky Elizabeth A. , Dow Steven 
Published: March 2023

AbstractRecent studies have highlighted a key role played by the sympathetic nervous system (SNS) and adrenergic stress in mediating immune suppression associated with chronic inflammation in cancer and other diseases. The connection between chronic SNS activation, adrenergic stress and immune suppression is linked in part to the ability of catecholamines to stimulate the bone marrow release and differentiation of myeloid‐derived suppressor cells (MDSC). Rodent model studies have revealed an important role for β‐adrenergic receptor signalling in suppression of cancer immunity in mice subjected to chronic stresses, including thermal stress. Importantly, therapeutic blockade of beta‐adrenergic responses by drugs such as propranolol can partially reverse the generation and differentiation of MDSC, and partly restore tumour immunity. Clinical trials in both humans and dogs with cancer have demonstrated that propranolol blockade can improve responses to radiation therapy, cancer vaccines and immune checkpoint inhibitors. Thus, the SNS stress response has become an important new target to relieve immune suppression in cancer and other chronic inflammatory conditions.
Prevalence and incidence of anal cancer in HIV-positive men: a systematic review protocol
Authors: Walker Holly , Palokas Michelle 
Published: March 2023

Objective:
The objective of this review is to synthesize the best evidence available to determine the prevalence and incidence of anal cancer in HIV-positive men.

Introduction:
Worldwide, an estimated 50,685 people were diagnosed with anal cancer in 2020 and an estimated 19,293 people died from the disease during the same year. From 2001 to 2015, the overall incidence of anal cancer increased by 2.7% per year, while mortality jumped by 3.1% each year. Evidence has shown that anal intraepithelial neoplasia can progress to cancer over time, particularly within the immunocompromised population.

Inclusion criteria:
This review will consider studies conducted in any setting, in any geographical location that report on the prevalence and incidence of adult males aged 18 years or older from any racial or ethnic background who are HIV positive and diagnosed with any type of anal cancer. Participants will be included regardless of stage of anal cancer, type of cancer treatment used, or length of time diagnosed.

Methods:
CINAHL, MEDLINE, Embase, LBGTQ+ Source (EBSCO), Web of Science Core Collection, MedNar, WorldWideScience, and ProQuest Dissertations and Theses databases will be searched from 1990 till the present. Analytical and descriptive observational studies will be included and critically appraised by 2 independent reviewers. Data will be extracted using JBI standardized data extraction tools. If sufficient data are available, a meta-analysis will be conducted; otherwise, the findings will be presented in narrative format, including tables and figures to aid in data presentation.

Review registration:
PROSPERO CRD42022327933
A change of heart: understanding the mechanisms regulating cardiac proliferation and metabolism before and after birth
Authors: Dimasi Catherine G. , Darby Jack R. T. , Morrison Janna L. 
Published: March 2023

AbstractMammalian cardiomyocytes undergo major maturational changes in preparation for birth and postnatal life. Immature cardiomyocytes contribute to cardiac growth via proliferation and thus the heart has the capacity to regenerate. To prepare for postnatal life, structural and metabolic changes associated with increased cardiac output and function must occur. This includes exit from the cell cycle, hypertrophic growth, mitochondrial maturation and sarcomeric protein isoform switching. However, these changes come at a price: the loss of cardiac regenerative capacity such that damage to the heart in postnatal life is permanent. This is a significant barrier to the development of new treatments for cardiac repair and contributes to heart failure. The transitional period of cardiomyocyte growth is a complex and multifaceted event. In this review, we focus on studies that have investigated this critical transition period as well as novel factors that may regulate and drive this process. We also discuss the potential use of new biomarkers for the detection of myocardial infarction and, in the broader sense, cardiovascular disease.
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Plasma membrane Ca2+ pump isoform 4 function in cell migration and cancer metastasis
Authors: Naffa Randa , Hegedűs Luca , Hegedűs Tamás , Tóth Sarolta , Papp Béla , Tordai Attila , Enyedi Ágnes 
Published: March 2023

AbstractThe Ca2+ ion is a universal second messenger involved in many vital physiological functions including cell migration and development. To fulfil these tasks the cytosolic Ca2+ concentration is tightly controlled, and this involves an intricate functional balance between a variety of channels and pumps of the Ca2+ signalling machinery. Among these proteins, plasma membrane Ca2+ ATPases (PMCAs) represent the major high‐affinity Ca2+ extrusion systems in the cell membrane that are effective in maintaining free Ca2+ concentration at exceedingly low cytosolic levels, which is essential for normal cell function. An imbalance in Ca2+ signalling can have pathogenic consequences including cancer and metastasis. Recent studies have highlighted the role of PMCAs in cancer progression and have shown that a particular variant, PMCA4b, is downregulated in certain cancer types, causing delayed attenuation of the Ca2+ signal. It has also been shown that loss of PMCA4b leads to increased migration and metastasis of melanoma and gastric cancer cells. In contrast, an increased PMCA4 expression has been reported in pancreatic ductal adenocarcinoma that coincided with increased cell migration and shorter patient survival, suggesting distinct roles of PMCA4b in various tumour types and/or different stages of tumour development. The recently discovered interaction of PMCAs with basigin, an extracellular matrix metalloproteinase inducer, may provide further insights into our understanding of the specific roles of PMCA4b in tumour progression and cancer metastasis.
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Supplemental Material: Oligocene-Miocene northward growth of the Tibetan Plateau: Insights from intermontane basins in the West Qinling Belt, NW China
Authors: Zhang Yi-Peng , Wang Weitao , al. et 
Published: March 2023

<p>Supplemental Text S1: Demagnetization and tests. Text S2: Anisotropy of magnetic susceptibility (AMS) analyses. Text S3: Detrital Zircons U-Pb Ages test. Figure S1: Anhua sampling section. Figure S2: Photographs of the sampled Anhua-Huicheng Basin section. Figure S3: Orthogonal (Zijderveld) vector plots of the representative thermal demagnetization behaviors of specimens from the Anhua section. Figure S4: Equal-area plots of accepted ChRMs (185 sites) from the Anhua section in (a) geographic and (b) tilt-corrected coordinates. Figure S5: AMS data. Table S1: Main characteristics and interpretations of major sedimentary facies in this study. Table S2: Fossils were discovered along the West Qinling Belt. Table S3: Zircon U-Pb geochronological analyses. Table S4: Quantitative comparison of age distributions for the Anhua Basin (Z1&Z2; Z3&Z4). </p>