bluuebunny/crossref_metadata_2025_split huggingface dataset:
Transcriptomic Landscape Of Colorectal Mucinous Adenocarcinoma Has Similarity With Intestinal Goblet Cells Differentiation
Authors: Liu Jianbo , Qiu Siyuan , Fu Xiaorui , Zhou Bin , Zu Ruijuan , Lv Zhaoying , Li Yuan , Yang Lie , Zhou Zongguang 
Published: June 2023

Abstract
Background
Mucinous adenocarcinoma (MC) of colorectal cancer (CRC) differs from adenocarcinoma (AD) in clinical features and molecular characteristics. Current treatment of colorectal MC isn't precise enough and the molecular characteristics remain unclear.
Methods
We accessed the data of CRC patients from The Cancer Genome Atlas (TCGA) database, then we performed differential analysis and weighted gene co-expression network analysis (WGCNA) to identify the differential hub RNAs between colorectal MC and AD. Functional enrichment analysis, RNAs co-expression networks, risk score based on least absolute shrinkage and selection operator (LASSO) regression model and validation in Gene Expression Omnibus (GEO) database, survival analysis were also performed. Finally, differential hub lncRNAs and hub RNA of significant module were validated by quantitative real time PCR (qRT-PCR) among different colon cancer cell lines.
Results
In total, we found 1680 differential expressed RNAs (DERs) and 4 significant modules (darkred, magenta, lightstellblue1, tan) comparing colorectal MC (52, 13.3%) with AD (340, 86.7%). From the functional enrichment analysis and RNAs co-expression networks, the darkred module was considered as a mucin-associated module, while others may be associated with other features of colorectal MC. Construction of logistic regression model and calculation of risk score based on differential hub RNAs in darkred module showed acceptable result in both TCGA and GEO data. Survival analysis suggested that many differential hub RNAs were positive and correlated with better survival. Finally, 8 differential hub RNAs in the darkred module (CTD-2547H18.1, CTD-2589M5.4, RP11-234B24.2, LA16c-321D4.2, LINC00261, RP11-25K19.1, COLCA1 and CAPN9) were validated by qRT-PCR. Except for LA16c-321D4.2 and COLCA1, all other RNAs showed higher expression levels in mucin-producing colorectal cell lines (Ls174T, HT-29 and T84).
Conclusion
The study strengthens the findings of distinct molecular features between MC and AD in CRC and identifies potential marker RNAs making colorectal MC unique from AD. The genesis of colorectal MC may be related to the differentiation fate of intestinal goblet cells.
Customized targeted massively parallel sequencing enables the identification of novel pathogenic variants in Tunisian patients with Developmental and Epileptic Encephalopathy
Authors: said Mariem Ben , Jallouli Olfa , Aissa Abir Ben , souissi Amal , Kamoun Fatma , Fakhfakh Faiza , Masmoudi Saber , Ayed Ikhlas Ben , triki Chahnez Charfi 
Published: June 2023

AbstractBackgroundDevelopmental and Epileptic Encephalopathies stand for a heterogenous group of epileptic syndromes, where the epileptic activity itself and/or the etiology contribute to cognitive and behavioral impairment. In recent decades, genetic etiology has increasingly been recognized as the cause of Developmental and Epileptic Encephalopathies and numerous genes have been identified, thanks to advances in genetic technologies. These discoveries have enabled precision treatments for several syndromes. Therefore, the identification of the causal variant in a gene is an intrinsic starting point to specify a precision therapy for the patient and an adequate management.ResultsWe developed a custom panel for Next Generation Sequencing of the coding sequences of 116 genes in individuals with Developmental and Epileptic Encephalopathy from the Tunisian population. Segregation analyses as well as in silico studies have been conducted to assess the identified variants’ pathogenicity. We report 12 pathogenic variants inSCN1A,CHD2,CDKL5,SZT2,KCNT1,GNAO1,PCDH19,MECP2,GRIN2A,andSYNGAP1in patients with Developmental and Epileptic Encephalopathy. Five of these variants are novel: “c.149delA, p.(Asn50MetfsTer26)” inCDKL5; “c.3616C>T, p.(Arg1206Ter)” inSZT2; “c.111_113del, p.(Leu39del)” inGNAO1; “c.1435G>C , p.(Asp479His)” inPCDH19;as well as “c.2143delC, p. (Arg716GlyfsTer10)”inSYNGAP1. Additionally, for five of our patients, the genetic result facilitated the choice of the appropriate treatment.ConclusionThis is the first report of a custom gene panel to identify genetic variants implicated in Developmental and Epileptic Encephalopathy in the Tunisian population as well as the North African region (Tunisia, Egypt, Libya, Algeria, Morocco) with a diagnostic rate of 30%. This high-throughput sequencing panel has considerably improved the rate of positive diagnosis of Developmental and Epileptic Encephalopathy in the Tunisian population, which was less than 15% using Sanger sequencing. The benefit of genetic testing in these patients was approved by both physicians and parents.
Characteristics of meiofaunal community in the subtidal zone near Hupo, anticipating ocean acidification in the East Sea of Korea
Authors: Oh Je Hyeok , Kang Teawook , Kim Dongsung , Shin Ayoung , Jung Min Gyu , Lee Wonchoel 
Published: June 2023

Abstract
This study aimed to investigate the meiofauna community characteristics in coastal waters highly affected by ocean acidification. Therefore, the meiofauna communities in the coastal waters of Hupo in Uljin-gun, a county bordering the East Sea of Korea, were monitored over five years. During the study period, the mean abundance of total meiofauna communities expressed in population density was 614.4 individuals (Inds.)/10 cm2, similar to the reported meiofauna abundance in the subtidal zone in the Yellow Sea of Korea, an area with sandy sedimentary facies. The most dominant taxa were nematodes (65–70%) and harpacticoids (7–20%); these two taxa accounted for approximately 80% of the total meiofauna abundance. Among the stations studied, station (St.) 10 showed the lowest seawater pH value, and in 2011, when the measured pH was the lowest at 7.82, St. 10 showed the lowest abundance values for total meiofauna and harpacticoids in the 5-year period. To examine the effect of ocean acidification on meiofauna communities at the species level, species of nematodes, the most dominant taxon, were analyzed. The results indicated that the number of nematode species at St. 10 in 2009, when the pH value was low, was 8, which was very low compared to that in the other years of the study period. According to the feeding type, epistrate feeders (2A) accounted for a remarkably high proportion at St. 10, which showed a low pH. This study provides various data on meiobenthic community characteristics to understand the effects of ocean acidification on coastal ecosystems.
Parenthood and Labor Market Outcomes: Evidence from Chile
Authors: Heggeness Misty L. , León Ana Sofía 
Published: June 2023

Abstract
Throughout the pandemic Chile implemented a series of public health mandates restricting mobility and high social-contact activities with a goal of reducing disease spread. In this paper, we study the impact of one of these policies-central planner variation in school reopenings on labor market outcomes. We examine how access to supervised care for children in school affected mothers’ labor supply and show that mothers increased labor force participation anywhere from 2.6 to 21.0 percentage points (ppts) as schools reopened. As mothers came back to the labor force, however, unplanned disruptions decreased their ability to stay actively engaged in work and increased their take up of leave from work, an artifact of unanticipated sick children and quarantine policies that particularly affected mothers who were secondary earners in the household. Conditional on being in the labor force, having a teenager buffered both mothers and fathers from work disruptions; parents were more likely to be actively working and less likely to be on leave. Our findings support a theory that parental labor supply is sensitivity to disruptions in the care of children but also depends on household composition and each parent’s role beyond gender. Policies that encourage consistent formalized systems of care and learning for children will not only benefit children, but also a second-earner’s ability to re-enter the labor force and advance at work.
JEL: J13, J16, J22
Biochemical assessment of some Red Sea brown algae with potential of antioxidant and antimicrobial agents
Authors: Hawas Usama W. , Hussein Sarah , El-Kassem Lamia T. Abou , Taie Hanan A.A. , El-Sherbiny Mohsen M. 
Published: June 2023

Abstract
The nutritional compositions of fatty acids (FAs), amino acids (AAs), polysaccharides, and phenolic compounds from brown seaweeds Cystoseira trinodis, Dictyota cervicornis, Padina gymnospora and Turbinaria oranata were studied, and their antioxidant and antimicrobial activities were evaluated. The lipophilic profile of D. cervicornis and T. oranata showed richness in saturated FAs (48.13 and 42.41%, respectively) mainly comprised of palmitic acid (16:0) with the proportions 38.4 and 33.79%, respectively. In contrast, C. trinodis and P. gymnospora revealed the highest amounts of unsaturated FAs (51.5 and 38.15%, respectively), constituted mainly of oleic acid (18:1, n9) with proportions 18.29 and 33.79%, respectively. Phytol, cholest-5-en-3-ol, β-stigmasterol and 9Z-octadecenamide were recorded as major unsaponifiable-lipid constituents. The major phenolic compounds were gallic acid in D. cervicornis, catechin in T. oranata and C. trinodis, and kaempferol in P. gymnospora with values of 227.54, 209.63, 167.18 and 85.82µg/g, respectively. The backbone of the polysaccharides in P. gymnospora was galactose (26.5%), ribose (38.9%) in C. trinode, mannose (49.7%) in D. cervicornis, and rhamnose (30.7%) in T. oranata. The major essential AAs were leucine and phenylalanine, while proline was the major non-essential AAs in all species. The MeOH extracts of T. oranata and C. trinodis displayed mild antioxidant activity with IC50 values of 26.1 and 30.5µg/ml. FAs contents of C. trinodis and D. cervicornis exhibited antibacterial activities against Staphylococcus aureus and Bacillus cereus, while FAs contents of T. oranata and P. gymnospora exhibited potent antifungal activities against Penicillium expansum and Syncephalastrum racemosum with inhibition MIC value for both = 0.312mg/ml.
Trends in antimicrobial resistance in Gaza Strip, 2020–2022
Authors: Mansour Hashem M. , Kurraz Rana A. , Rabii Ahmed Al , Masood Khalil I. 
Published: June 2023

Abstract
Background:
In this study, we analyzed the data on antimicrobial resistance in the bloodstream. This study aims to evaluate the bacterial resistance in the Gaza Strip and compare with that in other countries.
Methods:
This study was conducted retrospectively in the Indonesian and Shifa hospitals in the Gaza Strip of Palestine. The isolates were collected from 2020 to 2022 and compared with those from other countries. Chi square test to compare between means. P- value less than 0.05 was considered statistically significant
Results:
The incidences of bloodstream infections as well as the resistance against most antibiotics increased. The exceptions were a decreased incidence of P. aeruginosa infection and a decrease in resistance against ceftazidime. Overall, the incidence and resistance of antimicrobials were higher than other countries.
Conclusion:
These results showed increased trends to bacterial resistance except for ceftazidime. A specialist in clinical microbiology should take an active role in prescribing antibiotics in hospitals based on laboratory and epidemiological data besides clinical experience. Also, antibiotic stewardship should be constructed and activated in our country to, as this will decrease the emergence of resistant strains and decrease the burden
Mechanism of no-wear super-slip design of tribo-pair in load lifting and friction reduction
Authors: Hu Qiong , Wang Jinhua , Xiao Yang , Wang Yan , Wang Xiaolei , Zhou Jiawang 
Published: June 2023

Abstract
The concept of “super-slip design” is used to investigate the influence of multiple factors on the opening and frictional forces of a tribo-pair with microscale grooves. The results demonstrate that significantly improving the load-carrying capacity and reducing the viscous friction can be simultaneously realised by super-slip modification of the bottom surface of the hydrodynamic groove as a result of the joint action of the super-slip surface, groove geometrical parameters, and operating conditions. This is expected to further enhance the load-carrying capacity and reduce friction by applying shear stress on the interface of the liquid film and superslippery surface of the groove bottom. The superslippery surface does not fail because of rubbing, and the logical relationship between the performance parameters and groove depth changes unusually under this design.
Leaf-height-seed strategy of paired Meconopsis species and their impact factors along the elevational gradients in the south-eastern margin of Qinghai- Tibet Plateau
Authors: Shi Ning , Wang Jinniu , Zhang Lin , Wu Yan 
Published: June 2023

Abstract
Plant functional trait reflects plant growth information and resource utilization strategy. Leaf-height-seed (LHS) scheme is to quantify the strategy of plants by the location of three easily measurable functional traits in three-dimensional space. We applied this scheme to paired Meconopsis species to assess the variation of life-history traits over altitudinal gradients in the south-eastern margin of Qinghai-Tibet Plateau. LHS traits of 3 ~ 6 M. punicea and M. integrifolia populations had been measured. Furthermore, the impact of environmental factors on LHS traits had been addressed by regression models. We found both species displayed a significant decrease with increasing elevation for plant height and only the SLA of M. punicea varied significantly among elevations, while the elevation had no significant effect on seed mass. Moreover, most LHS traits have no significant relationships within species. Along the elevational gradient, the LHS strategies adopted by these two species varied to some extent. At high elevation, M. punicea tended to adopt conservative nutrient utilization strategies and enhance surviability, while M. integrifolia tended to adopt strategies that ensured rapid nutrient utilization and enhanced surviability. The most important environmental factor for their trait variation is the average growing season mean temperature. Variations in LHS traits of a single species suggested that plant strategy determined by the position within this three-factor triangle is not constant along the elevational gradient. Additionally, our study didn’t support some previously established cross-species correlations for interspecific patterns of LHS traits not widespread within species.
Friction and degradation of graphite: a nanotribological approach
Authors: Kim J. M. , Caron Arnaud 
Published: June 2023

Abstract
We investigated the friction and wear of graphite by atomic force microscopy in sliding contact with SiOx, Pt, and diamond tips with contact forces up to several micronewtons. Graphite’s tribology strongly depends on the chemistry of the counter body. With a SiOx tip, friction is governed by puckering. Wear initiates at surface steps by mechanical destabilization of folds. With a Pt tip, the adhesive effects lead to the exfoliation of graphite. At higher loads, friction crosses over from exfoliation to puckering. For SiOx and Pt, the wear rate is low in ambient conditions. In the case of diamond tips, we measured a friction coefficient and a wear rate of an order of magnitude larger than with SiOx or Pt tips.
Light and oxygen caused degradation of carbon nanotubes supported platinum catalyst in storage
Authors: Li Xiang , Li Lingling , Wu Yun , Cao Gengyu 
Published: June 2023

Abstract
Platinum (Pt) based catalysts are widely used in fuel cells due to its high catalytic activity. However, the high price and insatiability of Pt have hampered its commercial application. Enhancing the stability of Pt-based catalysts is very important. In this study, the degradation of Pt-based catalysts under storage conditions has been investigated. Both the oxygen and light will cause the degradation of Pt in storage conditions. However, the oxidation caused by light and oxygen alone is very slight, and the oxidation of Pt is obvious when oxygen and light work together.These findings provide a new sight in investigating the degradation mechanism of Pt-based catalysts.
Biomarkers Classification for Various Brain Disease using Artificial Intelligence Approach-A Study
Authors: Reeja S R , Mounika Sunkara , Mohanty Sachi Nandan 
Published: June 2023

Abstract

Diagnostic and prognostic markers for disease identification Furthermore impact of treatment on the illness continues to be a significant restriction in science. As a matter of fact, initial determination and guess of the sickness are hindered by a lack of suitable markers, and as a result, many lives are lost due to a variety of diseases because diagnosis occurs too late for a severe form of the illness because it is challenging to comprehend a recurrence. An illness’s medical condition can be positively identified using biomarkers. Diagnostic biomarkers may be crucial in this situation to make a precise diagnosis, identify individuals with a disease, and classify people with the same type of condition to tailor pharmacological therapies and boost therapeutic response. As a result, these biomarkers may be helpful for more than only diagnosing diseases; they may also be able to anticipate how a patient will respond to treatment. Research in the biology of disease is therefore heavily focused on finding appropriate and useful biomarkers for disease. Finding disease-specific biomarkers has recently been aided by deep learning. Brain functional connectivity (FC) changes may serve as biomarkers for forecasting several types of brain diseases. When the alterations are modest and there aren't any major structural changes overall, fMRI may be able to find abnormalities in the brain that other imaging methods can't. FMRI analytics are frequently used in various brain investigations, even clinical trials, in conjunction with deep learning models.
Electrochemical Label-free Methods for Ultrasensitive Multiplex
Protein Profiling of Infectious Diseases
Authors: Madhurantakam Sasya , Churcher Nathan Kodjo Mintah , Kumar Ruchita Mahesh , Prasad Shalini 
Published: June 2023

Abstract:
Electrochemical detection methods are the more appropriate detection methods
when it comes to the sensitive and specific determination of biomarkers. Biomarkers
are the biological targets for disease diagnosis and monitoring. This review focuses on recent
advances in label-free detection of biomarkers for infectious disease diagnosis. The
current state of the art for rapid detection of infectious diseases and their clinical applications
and challenges were discussed. Label-free electroanalytical methods are probably
the most promising means to achieve this. We are currently in the early stages of the
emerging technology of using label-free electrochemistry of proteins to develop biosensors.
To date, antibody-based biosensors have been intensively developed, although
many improvements in reproducibility and sensitivity are still needed. Moreover, there is
no doubt that a growing number of aptamers and hopefully label-free biosensors based
on nanomaterials will soon be used for disease diagnosis and therapy monitoring. And also
here in this review article, we have discussed recent developments in the diagnosis of
bacterial and viral infections, as well as the current status of the use of label-free electrochemical
methods for monitoring inflammatory diseases.
Dietary Components and Nutritional Strategies for Dementia Prevention in the Elderly
Authors: Cao Xi-Peng , Xu Wei , Wang Zuo-Teng , Tan Lan , Yu Jin-Tai 
Published: June 2023

Background:For decades, evidence from observational studies and randomized controlled trials has converged to suggest associations of dietary components, foods, and dietary patterns with dementia. With population aging and a projected exponential expansion of people living with dementia, formulating nutritional strategies for dementia prevention has become a research hotspot.Objective:This review aimed to summarize available data on the roles of specific dietary components, food groups, and dietary patterns in dementia prevention among the elderly.Methods:Database search was carried out using PubMed, the Cochrane Library, EMBASE, and Medline.Results:Polyphenols, folate, vitamin D, omega-3 fatty acids, and β-carotene might decrease the risk of dementia. Consumption of green leafy vegetables, green tea, fish, and fruits is recommended. However, saturated fat, a diet rich in both dietary copper and saturated fat, aluminum from drinking water, and heavy drinking might increase dementia risk. Healthy dietary patterns, especially the Mediterranean diet, were proven to bring more cognitive benefits than single dietary components.Conclusion:We discussed and summarized the evidence on the roles of dietary components and patterns in dementia prevention among the elderly and found that some factors were closely associated with dementia risk in elderly. This may pave the way for the identification of dietary components and patterns as new therapeutic targets for dementia prevention in the elderly population.
Investigating the Effects and Side Effects of Two Antipsychotic Drugs in
the Treatment of Children and Adolescents with Tourette Syndrome: A
Semi-experimental Research
Authors: Soltanifar Atefeh , Lashkarnevis Raheleh , Ziaee Maliheh , Moharari Fatemeh , Samadi Roya , Soltanifar Azadeh , Kamrani Maedeh 
Published: June 2023

Introduction:
Due to the high prevalence of Tourette's disorder among children and adolescents
and its negative consequences, an appropriate and effective medical treatment with minimal
complications is necessary. Therefore, this study was conducted to compare the effects of Aripiprazole
and Risperidone on Tourette's disorders in children and adolescents.

Method:
The statistical population of this semi-experimental study was children and adolescents aged
seven to eighteen years old. They were diagnosed with Tourette's disorder based on the DSM-V criteria
by the clinical interview of a child and adolescent psychiatrist in the child Psychiatry clinic of Ibne-
Sina's Psychiatric Hospital (Mashhad-Iran) in 2018. A total of forty participants were selected by the
convenience sampling method, and they were randomly divided into two groups treated with medicines,
Risperidone or Aripiprazole, for two months. Then, the demographic information questionnaire
was completed. The Y-GTSS Scale was completed. The clinical Effect Rating Scale (CGI-Tics Scale)
was completed. Calculation of body mass index and medical side effects complications were completed.
The evaluation was carried out at the beginning and on the second, fourth, and eighth weeks, and
the results were compared. The data were analyzed using SPSS software. 14, descriptive statistics,
Chi-square, and variance analysis.

Results:
The two groups were homogeneous in terms of demographic variables and body mass index.
Despite the positive effect of both medicines, no significant difference was observed among the general
scores of such disorders, the overall score of severity, Tourette's recovery, and BMI of these two
groups at the intervals and the end of treatments. (p <0.05). Due to the low number of complications
reported, statistical comparisons of the medical side effects were not made.

Conclusion:
According to the results, the two medicines, Aripiprazole and Risperidone, effectively improved
the symptoms of Tourette's disorder and its overall severity. However, there were no significant
statistical differences between them. Furthermore, in terms of the medical side effects, the statistical
comparison between the two medicines was impossible due to the small number of complications.