bluuebunny/crossref_metadata_2025_split huggingface dataset:
UPLC–MS/MS Method Development for Simultaneous Estimation of Diclofenac and Resveratrol-Loaded Liposomal Gel Formulation in Mice Skin Model: Application to Dermatokinetic Study
Authors: Alqahtani Safar M , Altharawi Ali , Alrobaian Majed , Almalki Waleed H , Alabbas Alhumaidi B , Ullah Shehla Nasar Mir Najib , Singh Tanuja , Thajudeen Kamal Younus , Khan Gyas , Rub Rehan A , Barkat Md Abul , Beg Sarwar , Rahman Mahfoozur 
Published: January 2023

Abstract
The current research work describes the development of a simple, fast, sensitive and efficient bioanalytical UPLC/MS–MS method for the simultaneous estimation of diclofenac and resveratrol in mice skin samples. Quetiapine was used as an internal standard (IS). Analytical separation was performed on ACQUITY UPLC C18 Column (2.1 × 100 mm; 1.7 μm) using ammonium acetate (5 mM) in water and methanol (B) with isocratic elution at ratio of (50, 50 v/v) and flow rate of 0.4 mL/min. The duration of separation was maintained for 3 min. Electrospray ionization mass spectrometry in a positive and negative ionization mode was used for detection. Selective ion mode monitoring was used for the quantification of m/z 296.025&gt; 249.93 for diclofenac, m/z 229.09 &gt; 143.03 for resveratrol and MRM/ES+ve mode applied in m/z 384.25&gt; 253.189 for IS transitions from parent to daughter ion. The lower detection and quantification limits were accomplished, and precision (repeatability and intermediate precision) with a coefficient of variation below 10% produced satisfactory results. The developed bioanalytical method was found to be useful for its suitability for the dermatokinetic evaluation of treatments through rat skin. Improvement in AUC (1.58-fold for diclofenac and 1.60-fold for resveratrol) and t1/2 in the dermis (2.13 for diclofenac and 2.21-fold for resveratrol) followed by epidermis was observed for diclofenac and resveratrol-loaded liposomal gel formulation over the conventional gel. Overall, the developed method for the dermatokinetic studies of the above-mentioned dual drugs-loaded liposome gel was found to be reproducible and effective for bioanalytical.
Could Home Human Immunodeficiency Virus Self-Testing Ever Become a Game Changer?
Authors: Wood Brian R , Stekler Joanne D 
Published: January 2023

Abstract
Could home human immunodeficiency virus (HIV) self-testing make a major difference in identifying persons with undiagnosed HIV in the United States? We argue that approval of new self-test assays for home use would help but must be combined with extensive investment in community outreach and linkage to care to make an impact.
Commutability Assessment of Candidate Reference Materials for Lipoprotein(a) by Comparison of a MS-based Candidate Reference Measurement Procedure with Immunoassays
Authors: Dikaios Ioannis , Althaus Harald , Angles-Cano Eduardo , Ceglarek Uta , Coassin Stefan , Cobbaert Christa M , Delatour Vincent , Dieplinger Benjamin , Grimmler Matthias , Hoofnagle Andrew N , Kostner Gerhard M , Kronenberg Florian , Kuklenyik Zsusanna , Lyle Alicia N , Prinzing Urban , Ruhaak L Renee , Scharnagl Hubert , Vesper Hubert W , Deprez Liesbet 
Published: January 2023

AbstractBackgroundElevated concentrations of lipoprotein(a) [Lp(a)] are directly related to an increased risk of cardiovascular diseases, making it a relevant biomarker for clinical risk assessment. However, the lack of global standardization of current Lp(a) measurement procedures (MPs) leads to inconsistent patient care. The International Federation for Clinical Chemistry and Laboratory Medicine working group on quantitating apolipoproteins by mass spectrometry (MS) aims to develop a next-generation SI (International system of units)-traceable reference measurement system consisting of a MS-based, peptide-calibrated reference measurement procedure (RMP) and secondary serum-based reference materials (RMs) certified for their apolipoprotein(a) [apo(a)] content. To reach measurement standardization through this new measurement system, 2 essential requirements need to be fulfilled: a sufficient correlation among the MPs and appropriate commutability of future serum-based RMs.MethodsThe correlation among the candidate RMP (cRMP) and immunoassay-based MPs was assessed by measuring a panel of 39 clinical samples (CS). In addition, the commutability of 14 different candidate RMs was investigated.ResultsResults of the immunoassay-based MPs and the cRMPs demonstrated good linear correlations for the CS but some significant sample-specific differences were also observed. The results of the commutability study show that RMs based on unspiked human serum pools can be commutable with CS, whereas human pools spiked with recombinant apo(a) show different behavior compared to CS.ConclusionsThe results of this study show that unspiked human serum pools are the preferred candidate secondary RMs in the future SI-traceable Lp(a) Reference Measurement System.
Diagnosis of Familial Dysbetalipoproteinemia Based on the Lipid Abnormalities Driven by APOE2/E2 Genotype
Authors: Bea Ana M , Cenarro Ana , Marco-Bened Victoria , Laclaustra Martn , Martn Csar , Ibarretxe Daiana , Pint Xavier , Arrobas Teresa , Vials Clara , Civeira Fernando , Olmos Salvador 
Published: January 2023

Abstract

Background
Familial dysbetalipoproteinemia (FDBL) is a monogenic disease due to variants in APOE with a highly variable phenotype. Current diagnostic lipid-based methods have important limitations. The objective is twofold: to define characteristics of dysbetalipoproteinemia (DBL) based on the analysis of APOE in patients from a lipid unit and in a sample from the general population, and to propose a screening algorithm for FDBL.

Methods
Lipids and APOE genotype from consecutive unrelated subjects from Miguel Servet University Hospital (MSUH) (n 3603), subjects from the general population participants of the Aragon Workers Health Study (AWHS) (n 4981), and selected subjects from external lipid units (Ext) (n 390) were used to define DBL criteria and to train and validate a screening tool.

Results
Thirty-five subjects from MSUH, 21 subjects from AWHS, and 31 subjects from Ext were APOE2/2 homozygous. The combination of non high-density lipoprotein cholesterol (non-HDLc)/apoB 1.7 plus triglycerides/apoB 1.35, in mg/dL (non-HDLc [mmol/L]/apolipoprotein B (apoB) [g/L] 4.4 and triglycerides [mmol/L]/apoB [g/L] 3.5), provided the best diagnostic performance for the identification of subjects with hyperlipidemia and APOE2/2 genotype (sensitivity 100 in the 3 cohorts, and specificity 92.8 [MSUH], 80.9 [AWHS], and 77.6 [Ext]). This improves the performance of previous algorithms. Similar sensitivity and specificity were observed in APOE2/2 subjects receiving lipid-lowering drugs.

Conclusions
The combination of non-HDLc/apoB and triglycerides/apoB ratios is a valuable tool to diagnose DBL in patients with hyperlipidemia with or without lipid-lowering drugs. FDBL diagnosis requires DBL and the presence of a compatible APOE genotype. Most adult APOE2/2 subjects express DBL, making FDBL as common as familial hypercholesterolemia in the population.
Rescue Therapies for Steroid-refractory Acute Severe Ulcerative Colitis: A Review
Authors: Gisbert Javier P , García María José , Chaparro María 
Published: January 2023

AbstractBackgroundOne-third of patients with acute severe ulcerative colitis [ASUC] are steroid-refractory. We aimed to review the different options for the management of steroid-refractory ASUC, including not only the standard treatment [cyclosporine and infliximab], but also most recently developed agents [such as vedolizumab, ustekinumab, and tofacitinib].MethodsWe performed a bibliographical search to identify studies focusing on the treatment of steroid-refractory ASUC.ResultsCyclosporine and infliximab currently represent the mainstays of salvage therapy and they are generally considered comparable. However, long-term persistence is higher in infliximab therapy, and many clinicians prefer to use infliximab given its ease of use. However, cost of cyclosporine is lower. Sequential rescue therapy after cyclosporine or infliximab failure [with infliximab and cyclosporine, respectively] could be considered in referral centres for highly selected cases. Tofacitinib, due to its rapid effect, represents an attractive rescue option mainly in biologic-experienced patients. The good safety profile of vedolizumab and ustekinumab makes them ideal candidates for use as maintenance therapy in combination with cyclosporine as induction therapy, especially for patients previously exposed to anti-TNFs or thiopurines.ConclusionsAlthough cyclosporine and infliximab still represent the mainstays of salvage therapy for steroid-refractory ASUC, new therapeutic agents may also play a role. Tofacitinib, due to its rapid effect, is an attractive therapeutic rescue option. Vedolizumab and ustekinumab, as maintenance therapy in combination with the fast-acting cyclosporine as induction therapy, may represent a promising bridging strategy, especially in patients with previous failure to thiopurines and/or anti-TNF agents.
Vaccines in Children Exposed to Biological AgentsIn Uteroand/or During Breastfeeding: Are They Effective and Safe?
Authors: Gisbert Javier P , Chaparro María 
Published: January 2023

AbstractBackgroundBiological agents cross the placenta, resulting in detectable blood levels in the neonate. Neonatal vaccinations are essential to prevent serious infections.AimTo review the effectiveness and safety of vaccines in children exposed to biological drugs in utero and/or those whose mothers received biological agents during lactation.MethodsA systematic bibliographic search was performed.ResultsInfants exposed in utero to anti-tumour necrosis factor drugs [anti-TNFs], vedolizumab or ustekinumab mount adequate serological responses to vaccines. No relevant adverse events for non-live inactivated vaccines have been reported in newborns exposed in utero to biologics. Studies assessing the safety of live-attenuated vaccines administered to infants exposed to biologics in utero have not observed, in general, serious adverse events. However, although no severe complications have been reported with rotavirus live vaccination, several fatal disseminated tuberculosis infections after administration of the BCG live vaccine in infants exposed to anti-TNFs in utero have been reported. Infliximab, adalimumab, and probably also vedolizumab and ustekinumab treatments are considered compatible with breastfeeding, although minuscule amounts of these biologics have been detected in breast milk of treated nursing mothers. Based on the literature available, the benefits from breastfeeding while receiving infliximab [or any other anti-TNF] and at the same time adhering to national infant immunization programmes probably outweigh any hypothetical risk for the infant.ConclusionsVaccines appear to be effective in infants exposed to biologics in utero. Inactivated vaccines are probably safe, whereas live-attenuated vaccines should be avoided while the children have detectable levels of biological drugs. Vaccines [non-live and live] are probably safe in children breastfed by mothers treated with biologics.
Single-cell RNA sequencing reveals transcriptional changes of human choroidal and retinal pigment epithelium cells during fetal development, in healthy adult and intermediate age-related macular degeneration
Authors: Collin Joseph , Hasoon Megan S R , Zerti Darin , Hammadi Sarah , Dorgau Birthe , Clarke Lucy , Steel David , Hussain Rafiqul , Coxhead Jonathan , Lisgo Steven , Queen Rachel , Lako Majlinda 
Published: January 2023

Abstract
Age-related macular degeneration (AMD) is the most prevalent cause of blindness in the developed world. Vision loss in the advanced stages of the disease is caused by atrophy of retinal photoreceptors, overlying retinal pigment epithelium (RPE) and choroidal endothelial cells. The molecular events that underline the development of these cell types from in utero to adult as well as the progression to intermediate and advanced stages AMD are not yet fully understood. We performed single-cell RNA-sequencing (RNA-Seq) of human fetal and adult RPE–choroidal tissues, profiling in detail all the cell types and elucidating cell type-specific proliferation, differentiation and immunomodulation events that occur up to midgestation. Our data demonstrate that progression from the fetal to adult state is characterized by an increase in expression of genes involved in the oxidative stress response and detoxification from heavy metals, suggesting a better defence against oxidative stress in the adult RPE–choroid tissue. Single-cell comparative transcriptional analysis between a patient with intermediate AMD and an unaffected subject revealed a reduction in the number of RPE cells and melanocytes in the macular region of the AMD patient. Together these findings may suggest a macular loss of RPE cells and melanocytes in the AMD patients, but given the complex processing of tissues required for single-cell RNA-Seq that is prone to technical artefacts, these findings need to be validated by additional techniques in a larger number of AMD patients and controls.
Management of testosterone around ovarian stimulation in transmasculine patients: challenging common practices to meet patient needs—2 case reports
Authors: Moravek Molly B , Dixon Marjorie , Pena Samantha M , Obedin-Maliver Juno 
Published: January 2023

AbstractApproximately 50% of transmasculine people use testosterone for gender affirmation, yet very little is known about the effects of testosterone on future reproductive capacity. Moreover, there are no data to guide fertility specialists on how to manage testosterone leading up to or during ovarian stimulation. Most clinics require cessation of testosterone prior to ovarian stimulation in this setting of no data; however, the current literature does suggest a potential increase in dysphoria with cessation of testosterone and during stimulation. This divergence begs the question of whether clinicians may be doing more harm than good by enacting this requirement. Here, we present two cases of transmasculine individuals who were on testosterone prior to stimulation and maintained their testosterone dosage throughout stimulation as proof of concept, followed by a discussion of current clinical practice and providing some rationale to support continuation of testosterone throughout stimulation.
Almost All Alternating Groups are Invariably Generated by Two Elements of Prime Order
Authors: Teräväinen Joni 
Published: January 2023

Abstract
We show that for all $n\leq X$ apart from $O(X\exp (-c(\log X)^{1/2}(\log \log X)^{1/2}))$ exceptions, the alternating group $A_{n}$ is invariably generated by two elements of prime order. This answers (in a quantitative form) a question of Guralnick, Shareshian, and Woodroofe.
Unicellular LLT polynomials and twins of regular semisimple Hessenberg varieties
Authors: Masuda Mikiya , Sato Takashi 
Published: January 2023

Abstract
The solution of Shareshian–Wachs conjecture by Brosnan–Chow linked together the cohomology of regular semisimple Hessenberg varieties and graded chromatic symmetric functions on unit interval graphs. On the other hand, it is known that unicellular LLT polynomials have similar properties to graded chromatic symmetric functions. In this paper, we link together the unicellular LLT polynomials and twin of regular semisimple Hessenberg varieties introduced by Ayzenberg–Buchstaber. We prove the palindromicity of LLT polynomials from topological viewpoint. We also show that modules of a symmetric group generated by faces of a permutohedron are related to a shifted unicellular LLT polynomial and observe the $e$-positivity of shifted unicellular LLT polynomials, which is established by Alexandersson–Sulzgruber in general, for path graphs and complete graphs through the cohomology of the twins.
Artistic Exceptionalism and the Risks of Activist Art
Authors: Earley Christopher 
Published: January 2023

Abstract
Activist artists often face a difficult question: is striving to change the world undermined when pursued through difficult and experimental artistic means? Looking closely at Adrian Piper’s Four Intruders Plus Alarm Systems (1980), I consider why this is an important concern for activist art and assess three different responses in relation to Piper’s work. What I call the ‘conciliatory stance’ recommends that when activist artists encounter misunderstanding, they should downplay their experimental artistry in favor of fitting their work to their audience’s appreciative capacities. What I call the ‘steadfast stance’ recommends that activist artists have reason to use their privilege of artistic exceptionalism to challenge their audience’s expectations, even if this leads to misunderstanding. I claim that a middle position, which I call ‘liberal conciliation’, best balances the demands for actual change placed on activism and the experimental means that artists bring to activism.
Analysis of user interactions with a digital health wallet for enabling care continuity in the context of an ongoing pandemic
Authors: Wachira Charles , Ogallo William , Okwako Sharon , Remy Sekou Lionel , Bukania Zipporah , Njeru Mercy Karimi , Mwangi Moses , Mokua Sharon , Omwanda Wycliffe , Ressler Daniele , Walcott-Bryant Aisha 
Published: January 2023

AbstractBackgroundThe onset of COVID-19 and related policy responses made it difficult to study interactive health informatics solutions in clinical study settings. Instrumented log and event data from interactive systems capture temporal details that can be used to generate insights about care continuity during ongoing pandemics.ObjectiveTo investigate user interactions with a digital health wallet (DHW) system for addressing care continuity challenges in chronic disease management in the context of an ongoing pandemic.Materials and methodsWe analyzed user interaction log data generated by clinicians, nurses, and patients from the deployment of a DHW in a feasibility study conducted during the COVID-19 pandemic in Kenya. We used the Hamming distance from Information Theory to quantify deviations of usage patterns extracted from the events data from predetermined workflow sequences supported by the platform.ResultsNurses interacted with all the user interface elements relevant to triage. Clinicians interacted with only 43% of elements relevant to consultation, while patients interacted with 67% of the relevant user interface elements. Nurses and clinicians deviated from the predetermined workflow sequences by 42% and 36%, respectively. Most deviations pertained to users going back to previous steps in their usage workflow.ConclusionsUser interaction log analysis is a valuable alternative method for generating and quantifying user experiences in the context of ongoing pandemics. However, researchers should mitigate the potential disruptions of the actual use of the studied technologies as well as use multiple approaches to investigate user experiences of health technology during pandemics.
Determination of Acephate, Dinotefuran, and Emamectin Benzoate in a Pesticide Formulation: A Single-Laboratory Validation
Authors: Patil Vilas K , Chandorkar Jayant G , Wagh Yogesh B , More Yogesh B , Narkhede Hemant P 
Published: January 2023

Abstract

Background
The advantage of simultaneous separation and quantification is the reduction of analysis time and consumption of solvents and reagents.

Objective
The objective of the present investigation was to optimize and validate a novel, rapid, and simple reverse-phase high-performance liquid chromatographic method for the simultaneous determination of acephate, dinotefuran, and emamectin benzoate in a pesticide formulation.

Method
The chromatographic separation and quantification were accomplished by using Kromasil CN column (250 mm × 4.6 mm; 5 μm) with a mobile phase consisting of acetonitrile and water [0.1% (v/v) triethylamine, pH 2.7 with 10% (v/v) orthophosphoric acid] in the ratio of 50:50 (v/v) with a flow rate of 1.0 mL/min and diode array detection at the wavelength of (215 nm and 245 nm).

Results
The HPLC method was able to separate and quantify all the actives in the formulation by isocratic elution within 10 min. The method was fully validated in accordance with the SANCO and Collaborative International Pesticide Analytical Council guidelines concerning system suitability, specificity, linearity, precision, accuracy, and robustness. All the analytical parameters are within the range of acceptable limits in the guidelines.

Conclusions
The validated method was successfully applied to a pesticide formulation.

Highlights
The novelty of the current research work lies in the development of the simple and rapid HPLC method for simultaneous determination of acephate, dinotefuran, and emamectin benzoate in wettable granular formulation.
Screening genes related to embryo implantation in Dazu black goats (Capra Hircus) by morphological and transcriptome analyses
Authors: Zhao Le , Yang Yongheng , Yang Haili , Luo Nanjian , Li Xingchun , Zheng Jikang , Yang Songjian , Zhao Yongju 
Published: January 2023

Abstract
Embryo implantation is a critical step in the establishment of pregnancy. However, the mechanisms of embryo implantation during early pregnancy in goats remain unclear due to the lack of published studies examining the genes involved in embryo implantation. As a popular goat breed in southwest China, Dazu black goats (DBGs) are highly adaptable and exhibit high fertility, making this breed a good model in which to study reproductive performance of goats. Here, morphological analysis showed that compared with the non-pregnant (NP) groups, the endometrial thickness of the goats in the P15 and P19 groups (15 and 19-day pregnant groups, respectively) were increased (P &lt; 0.01). Proliferating Cell Nuclear Antigen (PCNA) staining showed that PCNA was expressed in the NP, P15, and P19 groups. Transcriptome analysis was then conducted to identify gene expression patterns in uterine tissue during DBG embryo implantation. By comparing uterine tissue at different stages of embryonic implantation, 48 in NP_vs._P15, 318 in NP_vs._P19, and 1439 in P15_vs._P19, differentially expressed mRNAs were identified. Gene Ontology (GO) enrichments of the differentially expressed genes were enriched in the extracellular region, extracellular space, transporter activity, extracellular region, immune system process, immune response, and defense response etc. Through Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis, the biological metabolic pathways with which the differentially expressed genes are associated were explored. Through KEGG analysis, the DBGs were associated with oxidative phosphorylation, complement and coagulation cascades, arginine and proline metabolism, metabolic pathways, arachidonic acid metabolism, and ECM-receptor interaction. These candidate genes (CSF1, C1S, CST6, SLC24A4, HOXA10, HOXA11, MMP9, and ITGA11) and enriched signaling pathways could be valuable references for exploring the molecular mechanisms underlying goat embryo implantation.
Non-contingency in a paraconsistent setting
Authors: Kozhemiachenko Daniil , Vashentseva Liubov 
Published: January 2023

Abstract
We study an extension of first-degree entailment (FDE) by Dunn and Belnap with a non-contingency operator $\blacktriangle \phi $ which is construed as ‘$\phi $ has the same value in all accessible states’ or ‘all sources give the same information on the truth value of $\phi $’. We equip this logic dubbed $\textbf {K}^\blacktriangle _{\textbf {FDE}}$ with frame semantics and show how the bi-valued models can be interpreted as interconnected networks of Belnapian databases with the $\blacktriangle $ operator modelling search for inconsistencies in the provided information. We construct an analytic cut system for the logic and show its soundness and completeness. We prove that $\blacktriangle $ is not definable via the necessity modality $\Box $ of $\textbf {K}_{\textbf{FDE}}$. Furthermore, we prove that in contrast to the classical non-contingency logic, reflexive, $\textbf {S4}$ and $\textbf {S5}$ (among others) frames are definable.