bluuebunny/crossref_metadata_2025_split huggingface dataset:
Health risk assessment of heavy metals in marine fish caught from the northwest Persian Gulf
Authors: Torabi Solaleh , Gholizadeh Mohammad , Yazarlo Mahsa , Riahi Zahra 
Published: August 2023

Abstract

Due to the presence of metalloids and toxic metals such as cadmium, lead, copper, and zinc in fish tissue, it may endanger health considering the countless benefits of consuming fish, which can harm the human body if consumed in toxic amounts. Therefore, it is vital to monitor the concentration of metals in fish meat to ensure compliance with food safety regulations and, protect the consumer. We considered the levels of Ni, Zn, Cu, Pb, and Cd in 60 marine fish samples (3 species) collected from coastal areas of the northwestern coast of the Persian Gulf and estimated their health risk. Mean concentrations of Ni, Zn, Cu, Pb, and Cd were 1.88±0.07, 27.16±8.11, 11.55±4.12, 14±0.06, and 0.19 ± 0.03 µg/g wet weight. Estimated average daily intakes (EDIs) for adults and children of Ni, Zn, Cu, Pb, and Cd were 0.89-4.15, 12.89-60.02, 5.47-25.53, 0.54-2,51 and 0.09-0.42 μg/kg bw/day. Considering that there are different levels of certain elements in marine fish in the Persian Gulf, this study provides suggestions for risk management and a scientific basis for food safety assessment.
A multifunctional optoelectronic device based on 2D material with wide bandgap
Authors: Ding Baofu , Xu Hongwei , Liu Jingwei , Wei Sheng , Luo Jie , Gong Rui , Tian Siyuan , Yang Yiqi , Lei Yukun , Chen Xinman , Wang Jiahong , Zhong Gaokuo , Tang Yongbing , Wang Feng , Cheng Hui-Ming 
Published: August 2023

AbstractLow-dimensional materials exhibit unique quantum confinement effects and morphologies as a result of their nanoscale size in one or more dimensions, making them exhibit distinctive physical properties compared to bulk counterparts. Among all low-dimensional materials, due to their atomic level thickness, two-dimensional materials possess extremely large shape anisotropy and consequently are speculated to have large optically anisotropic absorption. In this work, we demonstrate an optoelectronic device based on the combination of two-dimensional material and quantum dot with wide bandgap. High-efficient luminescence of carbon quantum dot and extremely large shape anisotropy (>1500) of two-dimensional material with the wide bandgap of >4 eV cooperatively endow the optoelectronic device with multi-functions of optically anisotropic blue-light emission, visible light modulation, wavelength-dependent ultraviolet-light detection as well as blue fluorescent film assemble. This research opens new avenues for constructing multi-function-integrated optoelectronic devices via the combination of nanomaterials with different dimensions.
The role of antimicrobial prophylaxis in laparoscopic nephrectomy for renal cell carcinoma
Authors: Wei Mengchao , Yang Wenjie , Xu Weifeng , Liu Guanghua , Xie Yi , Dong Jie , Ji Zhigang 
Published: August 2023

Abstract
Background: Antimicrobial prophylaxis (AMP) is a regular perioperative procedure to prevent postoperative infection. However, the role and standard regimen of AMP in laparoscopic urologic surgery remain controversial. Our study aimed to investigate the role of AMP in laparoscopic nephrectomy for renal cell carcinoma (RCC).
Methods: We retrospectively enrolled 1000 patients who underwent laparoscopic nephrectomy from August 2019 to November 2021 in the Peking Union Medical College Hospital. Patients were divided into non-AMP (n = 444) group and AMP (n = 556) group. Outcomes including 30-day postoperative infection rate, the increase rate of pre- and post-operative white blood cell (WBC) counts and hospital stay were analyzed.
Results: The overall infection rate was 5.0% (28/556) in the AMP group, which was similar to 4.1% (18/444) in the non-AMP group (P= 0.461). The increase rate of pre- and post-operative WBC counts was significantly lower (85.5% versus 97.0%) in the AMP group (P = 0.004). The postoperative hospital stay was 5 (4, 6) days in both groups (P = 0.483). Logistic regression analyses identified the use of AMP had no influence on the occurrence of infection events [odds ratio (OR) = 0.797; 95% confidence interval (CI), 0.435–1.460; P = 0.462]. Hemoglobin (OR = 0.430; 95% CI, 0.257–0.719; P = 0.001) and partial nephrectomy (OR = 2.292; 95% CI, 1.724–3.046; P < 0.001) influenced the use of AMP independently.
Conclusions: The use of AMP had no impact on postoperative infection in patients receiving laparoscopic nephrectomy for RCC.
Analysis and improvement of human-induced vibration comfort of articulated steel pedestrian bridges
Authors: Yang Shuo , Ning Xiaojun 
Published: August 2023

Abstract
The effect of different pedestrian densities (0.2, 0.5, 1, 1.5 and 4.6 persons/m2) is studied, and the comfort level is evaluated according to the acceleration peak, and the most sensitive part of the acceleration response is set up with tuned mass dampers (TMD) for vibration control. The study shows that the bearing capacity level of the pedestrian bridge with articulated piers and columns meets the standard. Compared with the pier-column rigid connection, the structural dynamic characteristics of pier-column articulation do not change much, and the vertical frequency and peak acceleration in the span are slightly smaller. The comfort evaluation results are the same as those of the pier-column rigid pedestrian bridge. The setting of TMD can effectively reduce the human-induced vibration time response, and the vibration reduction efficiency can reach 52%, and the comfort level changes from CL2 to CL1 after vibration reduction.
A DNA damage repair signature predicted immunotherapy response and clinical prognosis in HNSCC
Authors: Ma Zhaoen , Liao Wenjing , Yang Saixuan , Song Lijuan , Zhang Xiaowen 
Published: August 2023

Abstract
Background:
DNA damage repair genes were confirmed to play important role in the tumorigenesis and tumor microenvironment. However, the role of DNA damage repair genes in Head and Neck Squamous Cell Carcinoma (HNSCC) is scarce
Method:
Gene expression profiles of The Cancer Genome Atlas (TCGA) HNSCC cohort was used to identify the DNA damage repair genes with survival prognosis. Least absolute shrinkage and selection operator (LASSO) regression was used to construct the prognosis model for HNSCC. Survival analysis and receiver operating curve for the HNSCC were used to validate the model. Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis were performed to identify the potential pathways associated with the signature model. Gene set enrichment analysis and Tumor Immune Dysfunction and Exclusion (TIDE) algorithm were used to investigate the tumor immune environment and immunotherapy response of HNSCC patients. Finally, nomogram was constructed to validate the clinical usefulness of the signature model.
Result:
We constructed a six-gene DNA damage repair related signature (including DCLRE1C, TOP3B, POLE2, POLD2, ERCC2 and RAD23B). The results of the survival analysis and ROC curve suggested that the signature showed good prognosis value. The risk score based on the signature model was significantly correlated with the immune cell infiltration and expression levels of the immune markers of HNSCC and could predict the immunotherapy response for HNSCC. Finally, a nomogram was constructed and showed good consistent fitness between the predicted and observed values for 1-, 3- and 5-year OS.
Conclusion:
We developed a DNA damage repair signature for HNSCC, which related to survival prognosis and tumor microenvironment, indicating its usage for clinical management.
Chaetoglobosin A induces T-24 apoptosis in human bladder cancer cells through oxidative stress and MAPK/PI3K-AKT-mTOR pathway
Authors: Song Jia , Qiao Jinyu , Chen Mingxue , Li Jiahui , Wang Jixia , Yu Dayong , Zheng Huachuan , Shi Liying 
Published: August 2023

Abstract
Chaetoglobosin A (ChA) is an antitumor compound produced by Chaetomium globosum. However, the mechanism of its antitumor effect has been rarely reported. In this study, we evaluated the anti-proliferative effect of ChA on human bladder cancer T-24 cells and explored its mechanism of action. ChA was found to have a good inhibitory effect on T-24 cells by MTT assay with an IC50 value of 48.14 ± 10.25 µΜ. Moreover, it was found to have a migration inhibitory ability and a sustained proliferation inhibitory effect on tumor cells by cell aggregation assay and cell migration assay. The appearance of apoptotic morphology was observed by Hoechst 33342 staining after ChA (25, 50, 75 µΜ) treatment, while Annexin V-FITC/PI double-staining assay also demonstrated that the number of apoptotic cells increased with the increase of drug concentration. Flow cytometry results showed that ChA treatment increased reactive oxygen species (ROS) and decreased mitochondrial membrane potential (MMP) in T-24 cells and inhibited cell mitosis, resulting in an increase in the number of sub-G1 phase cells. Further Western Blot experiments demonstrated that MAPK and PI3K-AKT-mTOR pathways were activated after drug treatment in addition to endogenous and exogenous apoptotic pathways. The addition of the ROS inhibitor N-acetylcysteine (NAC) upregulated the expression level of Bcl-2 protein, decreased p38 phosphorylation, increased ERK phosphorylation and restored the levels of PI3K and p-mTOR after ChA treatment. These suggest that regulation of oxidative stress, MAPK and PI3K-AKT-mTOR signaling pathways affect apoptosis.
Characteristics of HPV Integration in Cervical Adenocarcinoma and Squamous Carcinoma
Authors: Bi Yuxin , Hu Junbo , Zeng Ling , Chen Gang , Cao Huang , Wu Xufeng , Ma Quanfu 
Published: August 2023

Abstract
Purpose
HPV integration usually occur in HPV-related cancer, and is the main cause of cancer. But the carcinogenic mechanism of HPV integration is unclear. The study aims to provide a theoretical basis for understanding the pathogenesis of cervical adenocarcinoma (AC) and cervical squamous carcinoma (SCC).
Methods
We used HPV capture sequencing to obtain HPV integration sites in AC and SCC, and analyzed cytobands, distribution of genetic and genomic elements, identified integration hotspot genes, and performed pathway analysis.
Results
The results revealed that the most frequently observed integrated cytoband was 8q24.21 in AC and 21p11.2 in SCC, respectively. The breakpoints in both AC and SCC were more tended to occur within gene regions, compared to intergenetic regions. Compared to SCC samples, AC samples had a higher prevalence of genomic elements. Hotspot genes of HPV integration were STARD3 and ERBB2 in AC, and RNA45S rDNA and MIR3648-1 in SCC, respectively.
Conclusion
These results suggest that overexpression of viral oncogenes plays a significant role in the carcinogenesis of SCC. In AC, HPV integration may affect hosts’ oncogenes, and the dysregulation of oncogenes primarily contributes to progression of AC. Our analysis highlights STARD3 as a novel candidate biomarker of AC.
X Chromosome Rearrangement Associated with Premature Ovarian Insufficiency as Diagnosed by Molecular Cytogenetic Methods: A Case Reportand review of the literature
Authors: Peng Zhifang , Yang Renqi , Liu Qing , Chen Binbin , Long Panpan 
Published: August 2023

Abstract
Background: Premature ovarian insufficiency (POI) is a clinical condition characterized by ovarian dysfunction in women under 40. The etiology of most POI cases remains unidentified and is believed to be multifactorial, including factors such as autoimmunity, metabolism, infection, and genetics. POI exhibits significant genetic heterogeneity, and it can result from chromosomal abnormalities and monogenic defects.
Case presentation: The study participant, a 33-year-old woman, presented with a history of irregular menstruation that commenced two years ago, progressing to prolonged menstrual episodes and eventual cessation. The participant exhibits a rearrangement of the X chromosome, characterized by heterozygosity duplication on the long arm and heterozygosity deletion on the short arm by whole exome sequencing(WES) combined with cell chromosome detection.
Conclusions: This study expands the spectrum of mutations associated with POI resulting from X chromosomal abnormalities. WES-Copy number variation (CNV) analysis, in conjunction with chromosome karyotype analysis and other detection techniques, can provide a more comprehensive understanding of the genetic landscape underlying complex single or multi-system diseases.
Does bilateral Wilms' tumor involving the collecting system in children have a worse prognosis?
Authors: Fang YiWei , Sun Ning , Song HongCheng , Zhang WeiPing , Li Ning , Yang Yang 
Published: August 2023

Abstract
Objective
To summarize the clinical characteristics, treatment methods and prognosis of bilateral Wilms' tumor (BWT) in children involving the collecting system.
Methods
Patients with BWT admitted to our pediatric surgery center between January 2008 and June 2022 were reviewed. Children were grouped according to the relationship between the tumor and the collecting system. Group Ⅰ included children with tumor infiltrating the collecting system, group Ⅱ included children with tumor growing into the collecting system, and group Ⅲ included children whose tumor did not involve the collecting system. The clinical features, treatments and prognosis of the patients were analyzed.
Results
Seventy patients were enrolled, including 20 patients with 25 sides of tumors infiltrating the collecting system in group Ⅰ,10 patients with 13 sides of tumors growing into the collecting system in group Ⅱ, and 40 patients in group III. There was no significant difference in patients age and gender between group I and group II. In total, 20 patients in group Ⅰ and 9 patients in group II had partial response (PR) after neoadjuvant chemotherapy. In group I, 22 of 25 sides of tumors underwent nephron-sparing surgery (NSS); in group Ⅱ, 11 of 13 sides of tumors underwent NSS. During an average follow-up of 47 months, in group I, 6/20 patients relapsed and 2/20 patients died; in group Ⅱ, 3/10 patients relapsed and 1/10 patient died. There was no significant difference in 4-year overall survival (OS) rate among groups I, Ⅱ and Ⅲ(86.36% vs. 85.71%vs. 91.40%, P = 0.902).
Conclusions
To preserve renal parenchyma, NSS is feasible for children with BWT involving the collecting system. There was no significant difference in postoperative long-term OS between patients with BWT involving the collecting system and not involving the collecting system.
Analysis of land use/cover changes using the concepts of ecosystem services for the ecological security assessment of Lavasanat watershed (Tehran, Iran)
Authors: moarrab Yasser , Amiri Mohammad Javad , Jadidi Mahnaz 
Published: August 2023

Abstract

Recent years have seen growing interest in the use of the concepts of ecosystem services in ecological security assessments. This study analyzed the temporal-spatial changes of land use/cover in Lavasanat watershed, Tehran, Iran, between the years 2000 and 2040 under different land-use change scenarios, and assessed the consequent change in ecological security in terms of ecosystem services (water yield) under different climate scenarios. First, the LULC map of the watershed for the 20 year period between 2000 and 2020 was generated. Then, the area’s LULC changes from 2020 to 2040 were predicted based on the assumption of the continuation of current conditions, which was named Scenario S1. Next, three other scenarios named S2, S3, and S4, representing the pessimistic, realistic, and optimistic management of the situation were defined based on S1. Three climate scenarios named B1, N and M were also defined for use in the assessments. For ecological security assessments, temporal and spatial changes of water yield services for the years 2000, 2010 and 2020 were simulated based on the area’s climate data and LULC changes. The results were then used to estimate the water yield of the entire watershed and its residential areas by 2040 in the four defined land-use/cover scenarios (S1, S2, S3, and S4) and three climate scenarios (B1, N, and M). The results showed a 1.92 times increase in the water yield of the watershed’s residential areas from 2000 to 2020. Among the scenarios defined for Lavasanat watershed, the worst scenario (with the highest water yield) was S2N with a water yield of 37639293.581m3 in the entire watershed and 35091082.311m3 in the residential areas, and the best scenario (with the lowest water yield) was S4M with a water yield of 8329365.079m3 in the entire watershed and 7353006.022m3 in the residential areas. The results of all of the four land-use/cover scenarios under all three climate scenarios showed an increase in water yield, the expansion of residential areas, and the loss of ecologically valuable lands in the watershed by 2040.
Invasive FoxM1 phosphorylated by PLK1 induces the polarization of tumor-associated macrophages to promote immune escape and metastasis, amplified by IFITM1
Authors: Xu Rong , Lee Young-Joo , Kim Chang-Hyeon , Min Ga-Hong , Kim Yeo-Bin , Park Jung-Won , Kim Dae-Hoon , Kim Jung-Hyun , Yim Hyungshin 
Published: August 2023

Abstract
Background
Understanding the mechanism behind immune cell plasticity in cancer metastasis is crucial for identifying key regulators. Previously we found that mitotic factors regulate epithelial-mesenchymal transition, but how these factors convert to metastatic players in the tumor microenvironment (TME) is not fully understood.
Methods
The clinical importance of mitotic factors was analyzed by heatmap analysis and a KM plot in lung adenocarcinoma (LUAD) patients. Immunoprecipitation, LC-MS/MS, kinase assay, and site-directed mutagenesis were performed for the interaction and phosphorylation. A tail-vein injection mouse model, Transwell-based 3D culture, microarray analysis, coculture with monocytes, and chromatin immunoprecipitation assays were used to elucidate the function of phosphorylated FoxM1 in metastasis of TME.
Results
The phosphorylated FoxM1 at Ser25 by PLK1 acquires the reprogramming ability to stimulate the invasive traits in cancer and influence immune cell plasticity. This invasive form of p-FoxM1 upregulates the expression of IL1A/1B, VEGFA, and IL6 by direct activation, recruiting monocytes and promoting the polarization of M2d-like tumor-associated macrophages (TAMs). Upregulation of PD-L1 in LUAD having phosphomimetic FoxM1 facilitates immune evasion. In invasive LUAD with phosphomimetic FoxM1, IFITM1 is the most highly expressed through the activation of the STING-TBK1-IRF3 signaling, which enhances FoxM1-mediated signaling. Clinically, higher expression of FOXM1, PLK1, and IFITM1 is inversely correlated with the survival rate of advanced LUAD patients, providing a promising therapeutic strategy for the treatment of LUAD.
Conclusion
FoxM1-based therapy would be a potential therapeutic strategy for LUAD to reduce TAM polarization, immune escape, and metastasis, since FoxM1 functions as a genetic reprogramming factor reinforcing LUAD malignancy in the TME.
Characteristics of pulmonary artery catheter use in multicenter ICUs in Japan and the association with mortality: a multicenter cohort study using the Japanese Intensive care PAtient Database
Authors: Fukano Kentaro , Iizuka Yusuke , Nishiyama Seiya , Yoshinaga Koichi , Uchino Shigehiko , Sasabuchi Yusuke , Sanui Masamitsu 
Published: August 2023

Abstract
Background: It has been 50 years since the pulmonary artery catheter was introduced, but the actual use of pulmonary artery catheters in recent years is unknown. Some randomized controlled trials have reported no causality with mortality, but some observational studies have been published showing an association with mortality for patients with cardiogenic shock, and the association with a pulmonary artery catheter and mortality is unknown. The aim of this study was to investigate the utilization of pulmonary artery catheters (PACs) in the intensive care unit (ICU) and to examine their association with mortality, taking into account differences between hospitals.
Methods: This is a retrospective analysis using the Japanese Intensive care PAtient Database (JIPAD), a multicenter, prospective, observational registry in Japanese ICUs. We included patients aged 16 years or older who were admitted to the ICU for reasons other than procedures. We excluded patients who were discharged within 24 hours, or had missing values. We compared the prognosis of patients with and without PAC. The primary outcome was hospital mortality. We performed propensity score analysis to adjust for baseline characteristics and hospital characteristics.
Results: Among 184,705 patients in this registry from April 2015 to December 2020, 59,922 patients were included in the analysis. Most patients (94.0%) using PAC had cardiovascular disease. There was a wide variation in the frequency of PAC use between hospitals, from 0% to 60.3% (median 14.4%, interquartile range 2.2 to 28.6%). Hospital mortality was not significantly different between the PAC use group and the non-PAC use group in patients after adjustment for propensity score analysis (3.9% vs 4.3%; difference, -0.4%; 95% CI, -1.1 to 0.3; p=0.32). Among patients with cardiac disease, those with post open-heart surgery and those in shock, hospital mortality was also not significantly different between the two groups (3.4% vs 3.7%, p = 0.45, 1.7% vs 1.7%, p = 0.93, 4.8% vs 4.9%, p = 0.87).
Conclusions: The frequency of PAC use varied among hospitals. PAC use for ICU patients was not associated with lower hospital mortality after adjusting for differences between hospitals.
Peptidomimetics for CVD screened via TRADD-TRAF2 complex interface assessments
Authors: Alagumuthu Manikandan , S Jeevitha , Vusa Laharika 
Published: August 2023

Abstract
This study aimed to screen and develop a few Peptidomimetics to treat Cardio Vascular Disease (CVD). Designed peptidomimetics from peptides generated from the protein interacting region of TRADD (Tumor necrosis factor receptor type 1-associated DEATH domain protein) and TRAF2 (TNF receptor-associated factor 2) complex which is critical in Atherosclerosis (AS) pathogenesis as it assists in a series of signal transducers that activates NF-κB. The triggered NF-κB makes an extensive amount of nitric oxide (NO) synthesized by inducible nitric oxide synthase (iNOS), which boons the progress of AS. The examined TRADD-TRAF2 complex (PDB ID: 1F3V) from the previous study indicates that the sequence range W11-G165 of TRADD highly interacts with TRAF2. The sequence was selected for inhibitory peptide design. Further, the selected sequence was mutated with the alanine sequence to have a variety of peptide ranges. And with the help of different silicon tools, the top three, MIP11-25l, MIP131-143h, and MIP149-164m showed the best interaction with critical residue. pepMMsMIMIC is a virtual screening tool mainly based on a multi-conformers three-dimensional (3D)-similarity search strategy. 600 peptidomimetics were identified & retrieved for further screening over molecular docking and MD (Molecular Dynamics) simulations. Density Functional Theory (DFT) and ADMET predictions also applied in order to validate the screened peptidomimetics compounds druggability. In the results, pepmimic compounds MMs03918858 and MMs03927281 with binding energy values of -9.6 kcal/mol and − 9.1 kcal/mol respectively were screened as best and are proposed for further pre-clinical studies.