bluuebunny/crossref_metadata_2025_split huggingface dataset:
Real‐world experience with Curcumin–QingDai combination for patients with active ulcerative colitis: A retrospective multicentre cohort study
Authors: Yanai Henit , Salomon Nir , Lahat Adi , Ungar Bella , Eliakim Rami , Kriger‐Sharabi Ofra , Reiss‐Mintz Hilla , Koslowsky Benjamin , Shitrit Ariella Bar‐Gil , Tamir‐Degabli Natalie , Dotan Iris , Zittan Eran , Maharshak Nitsan , Hirsch Ayal , Ben‐Horin Shomron , Kopylov Uri 
Published: May 2023

SummaryBackgroundCurcumin and QingDai (QD, Indigo) have been shown to be effective for treating active ulcerative colitis (UC).AimTo evaluate the real‐world experience with the Curcumin–QingDai (CurQD) herbal combination to induce remission in active UC.MethodsA retrospec‑tive multicentre adult cohort study from five tertiary academic centres (2018–2022). Active UC was defined as a Simple Clinical Colitis Activity Index (SCCAI) ≥ 3. Patients were induced by CurQD. The primary outcome was clinical remission at weeks 8–12, defined as SCCAI ≤2 and a decrease ≥3 points from baseline. Secondary outcomes were clinical response (SCCAI decrease ≥3 points), corticosteroid‐free remission, faecal calprotectin (FC) response (reduction ≥50%), FC normalisation (FC ≤100 μg/g for patients with FC ≥300 μg/g at baseline), and safety. All outcomes were analysed for patients who were maintaining stable treatment.ResultsEighty‐eight patients were included; 50% were biologics/small molecules experienced, and 36.5% received ≥2 biologics/small molecules. Clinical remission was achieved in 41 (46.5%), and clinical response in 53 (60.2%). Median SCCAI decreased from 7 (IQR:5–9) to 2 (IQR:1–3); p < 0.0001. Of the 26 patients on corticosteroids at baseline, seven achieved corticosteroid‐free remission. Among 43 biologics/small molecules experienced patients, clinical remission was achieved in 39.5% and clinical response in 58.1%. FC normalisation and response were achieved in 17/29 and 27/33, respectively. Median FC decreased from 1000 μg/g (IQR:392–2772) at baseline to 75 μg/g (IQR:12–136) at the end of inductions (n = 30 patients with paired samples); p < 0.0001. No overt safety signals emerged.ConclusionIn this real‐world cohort, CurQD effectively induced clinical and biomarker remission in patients with active UC, including patients who were biologics/small molecules experienced.
Detection and verification of neurodegeneration after traumatic brain injury in the mouse: Immunohistochemical staining for amyloid precursor protein
Authors: Xiong Guoxiang , Metheny Hannah , Hood Kaitlin , Jean Ian , Farrugia Anthony M. , Johnson Brian N. , Tummala Shanti R. , Cohen Noam A. , Cohen Akiva S. 
Published: May 2023

AbstractPrevious studies of human traumatic brain injury (TBI) have shown diffuse axonal injury as varicosities or spheroids in white matter (WM) bundles when using immunoperoxidase‐ABC staining with 22C11, a mouse monoclonal antibody against amyloid precursor protein (APP). These findings have been interpreted as TBI‐induced axonal pathology. In a mouse model of TBI however, when we used immunofluorescent staining with 22C11, as opposed to immunoperoxidase staining, we did not observe varicosities or spheroids. To explore this discrepancy, we performed immunofluorescent staining with Y188, an APP knockout‐validated rabbit monoclonal that shows baseline immunoreactivity in neurons and oligodendrocytes of non‐injured mice, with some arranged‐like varicosities. In gray matter after injury, Y188 intensely stained axonal blebs. In WM, we encountered large patches of heavily stained puncta, heterogeneous in size. Scattered axonal blebs were also identified among these Y188‐stained puncta. To assess the neuronal origin of Y188 staining after TBI we made use of transgenic mice with fluorescently labeled neurons and axons. A close correlation was observed between Y188‐stained axonal blebs and fluorescently labeled neuronal cell bodies/axons. By contrast, no correlation was observed between Y188‐stained puncta and fluorescent axons in WM, suggesting that these puncta in WM did not originate from axons, and casting further doubt on the nature of previous reports with 22C11. As such, we strongly recommend Y188 as a biomarker for detecting damaged neurons and axons after TBI. With Y188, stained axonal blebs likely represent acute axonal truncations that may lead to death of the parent neurons. Y188‐stained puncta in WM may indicate damaged oligodendrocytes, whose death and clearance can result in secondary demyelination and Wallerian degeneration of axons. We also provide evidence suggesting that 22C11‐stained varicosities or spheroids previously reported in TBI patients might be showing damaged oligodendrocytes, due to a cross‐reaction between the ABC kit and upregulated endogenous biotin.
LIPUS‐SCs‐Exo promotes peripheral nerve regeneration in cavernous nerve crush injury‐induced ED rats via PI3K/Akt/FoxO signaling pathway
Authors: Ye Kun , Li Zitaiyu , Yin Yinghao , Zhou Jun , Li Dongjie , Gan Yu , Peng Dongyi , Xiao Ming , Zhao Liangyu , Dai Yingbo , Tang Yuxin 
Published: May 2023

AbstractObjectiveClinical treatment of erectile dysfunction (ED) caused by cavernous nerve (CN) injury during pelvic surgery is difficult. Low‐intensity pulsed ultrasound (LIPUS) can be a potential strategy for neurogenic ED (NED). However, whether Schwann cells (SCs) can respond to LIPUS stimulation signals is unclear. This study aims to elucidate the signal transmission between SCs paracrine exosome (Exo) and neurons stimulated by LIPUS, as well as to analyze the role and potential mechanisms of exosomes in CN repair after injury.MethodsThe major pelvic ganglion (MPG) neurons and MPG/CN explants were stimulated with LIPUS of different energy intensities to explore the appropriate LIPUS energy intensity. The exosomes were isolated and purified from LIPUS‐stimulated SCs (LIPUS‐SCs‐Exo) and non‐stimulated SCs (SCs‐Exo). The effects of LIPUS‐SCs‐Exo on neurite outgrowth, erectile function, and cavernous penis histology were identified in bilateral cavernous nerve crush injury (BCNI)‐induced ED rats.ResultsLIPUS‐SCs‐Exo group can enhance the axon elongation of MPG/CN and MPG neurons compared to SCs‐Exo group in vitro. Then, the LIPUS‐SCs‐Exo group showed a stronger ability to promote the injured CN regeneration and SCs proliferation compared to the SCs‐Exo group in vivo. Furthermore, the LIPUS‐SCs‐Exo group increased the Max intracavernous pressure (ICP)/mean arterial pressure (MAP), lumen to parenchyma and smooth muscle to collagen ratios compared to the SCs‐Exo group in vivo. Additionally, high‐throughput sequencing combined with bioinformatics analysis revealed the differential expression of 1689 miRNAs between the SCs‐Exo group and the LIPUS‐SCs‐Exo group. After LIPUS‐SCs‐Exo treatment, the phosphorylated levels of Phosphatidylinositol 3‐kinase (PI3K), protein kinase B (Akt) and forkhead box O (FoxO) in MPG neurons increased significantly compared to negative control (NC) and SCs‐Exo groups.ConclusionOur study revealed that LIPUS stimulation could regulate the gene of MPG neurons by changing miRNAs derived from SCs‐Exo, then activating the PI3K‐Akt‐FoxO signal pathway to enhance nerve regeneration and restore erectile function. This study had important theoretical and practical significance for improving the NED treatment.
Multicentre, prospective, randomized control non‐inferiority trial of bladder catheter management in colon surgery
Authors: Serra‐Aracil Xavier , Hidalgo Jose M. , Dominguez Arturo , Vallverdu Helena , Millan Monica , Caro‐Tarragó Aleidis , Delgado Salvadora , Gomez C. , Llorach Nuria , Mora‐Lopez Laura ,   the CR‐Vesical Cath‐Study group
Published: May 2023

AbstractAimPerioperative bladder catheterization is a controversial issue. Most current recommendations are based on data from open surgery extrapolated to enhanced recovery after surgery or fast‐track programmes ranging between 24 and 48 h. The aim of this study is to provide a rationale for reducing catheterization time while at the same time avoiding acute urine retention (AUR), in patients undergoing scheduled laparoscopic colon surgery.MethodThis is a multicentre, prospective, controlled, randomized non‐inferiority study of bladder catheter management in patients undergoing scheduled laparoscopic colon surgery, randomized into two groups: experimental (with catheter removal immediately after surgery) and control (with catheter removal 24 h post‐surgery). The main outcome will be the development of AUR, and secondary outcomes the development of urinary infection within the first 30 days and hospital stay. Demographic, surgical and pathological variables will also be evaluated, especially the development of adverse effects assessed according to the Clavien scale and the Comprehensive Complication Index. Following the literature, we assume an incidence of AUR of 11% and a margin of non‐inferiority (delta) of 8% and estimate that a sample size of 208 patients per group will be required (with an estimated 10% of losses per group).ConclusionsIn this study we try to demonstrate that the bladder catheter can be removed immediately after scheduled laparoscopic colon surgery, without increasing acute urine retention. This measure would offers the benefits of earlier mobilization and reduces catheter‐related morbidity.
Digital technologies: Innovations that transform the face of drug development
Authors: Izmailova Elena S. , AbuAsal Bilal , Hassan Hazem E. , Saha Anindita , Stephenson Diane 
Published: May 2023

AbstractRecently, digital health technologies (DHTs) and digital biomarkers have gained a lot of traction in clinical investigations, motivating sponsors, investigators, and regulators to discuss and implement integrated approaches for deploying DHTs. These new tools present new and unique challenges for optimal technology integration in clinical trial processes, including operational, ethical, and regulatory issues. In this paper, we gathered different perspectives to discuss challenges and perspectives from three different stakeholders: industry, US regulators, and a public‐private partnership consortium. The complexities of DHT implementation, which include regulatory definitions, defining the scope of validation experiments, and the need for partnerships between BioPharma and the technology sectors, are highlighted. Most of these challenges are related to translation of DHT‐derived measures into endpoints that are meaningful to clinicians and patients, participant safety, training, and retention and privacy of data. The example of the Wearable Assessments in the Clinic and Home in PD (WATCH‐PD) study is discussed as an example that demonstrated the advantages of pre‐competitive collaborations, which include early regulatory feedback, data sharing, and multistakeholder alignment. Future advances in DHTs are expected to spur device‐agnostic measured development and incorporate patient reported outcomes in drug development. More efforts are needed to define validation experiments for a defined context of use, incentivize data sharing and development of data standards. Multistakeholder collaborations via precompetitive consortia will help facilitate broad acceptance of DHT‐enabled measures in drug development.
A meta‐analytic review of the effectiveness of psychological treatment of functional/dissociative seizures on non‐seizure outcomes in adults
Authors: Gaskell Chris , Power Niall , Novakova Barbora , Simmonds‐Buckley Melanie , Reuber Markus , Kellett Stephen , Rawlings Gregg H. 
Published: May 2023

AbstractPsychological therapies are considered the treatment of choice for functional/dissociative seizures (FDSs). Although most previous studies have focused on seizure persistence or frequency, it has been argued that well‐being or health‐related quality of life outcomes may actually be more meaningful. This study contributes by summarizing and meta‐analyzing non‐seizure outcomes to quantify the effectiveness of psychological treatment in this patient group. A pre‐registered systematic search identified treatment studies (e.g., cohort studies, controlled trials) in FDSs. Data from these studies were synthesized using multi‐variate random‐effects meta‐analysis. Moderators of treatment effect were examined using treatment characteristics, sample characteristics, and risk of bias. A total of 171 non‐seizure outcomes across 32 studies with a pooled sample size of N = 898 yielded a pooled effect‐size of d = .51 (moderate effect size). The outcome domain assessed and the type of psychological treatment were significant moderators of reported outcomes. Greater rates of improvement were demonstrated for outcomes assessing general functioning. Behavioral treatments emerged as particularly effective interventions. Psychological interventions are associated with clinical improvements across a broad array of non‐seizure outcomes, over and above seizure frequency, in adults with FDSs.
Effect of ultrasonic treatment on soybean okara to be used as a gluten‐free bread improver
Authors: Moscoso Ospina Yeisson A , Lionello Melina E. , Garófalo Luciana , Burbano Juan J. , Cabezas Dario M. , Correa M. Jimena 
Published: May 2023

SummaryThe production of soybean protein isolates generates an insoluble residue called okara that is rich in proteins and dietary fibre. These macromolecules have functional properties that can be modified and improved by emerging technologies, such as high‐intensity ultrasound. The objectives of this work were to analyse the ability of soybean okara (UOK) to act as a gluten‐free bread improver and to evaluate the effect of a sonication process on this property (OKS). Both okaras were assayed at two percentages (2% and 3%) leading to a reinforcement of interactions in the matrix of batters, producing bread of better technological and sensorial quality. The addition of OKS 3%, improved specific volume (20%), reduced the hardness (30%) and increased the cohesiveness of the crumb and improved the air inclusion. These results demonstrated the efficiency of the sonication process to obtain a gluten‐free bread improver from a bulky by‐product of the soybean industry.
Fabrication of pea protein–naringin Pickering emulsion to mask the bitterness of naringin
Authors: Tian Mengwei , Wang Jin , Hayat Khizar , Lu Hui , Cong Lixia , Huang Meigui 
Published: May 2023

SummaryNaringin has various biological activities with an unacceptable bitter taste. In this work, pea protein–naringin complexes (PP–NG complexes) were constructed to stabilise the Pickering emulsion and reduce the bitterness of naringin. Sensory evaluation results showed that the bitterness and after‐bitterness of the PP–NG complexes were reduced by 77.78% and 75.99% compared with 1 mm naringin respectively. For the PP–NG complexes, the fluorescence spectra results showed that the endogenous fluorescence intensity of pea protein decreased with the naringin concentration increased (0–2 mm), and each pea protein molecule bound to one naringin molecule. Results obtained via thermodynamic analysis molecular docking demonstrated that naringin had an excellent binding affinity (−7.4 kcal mol−1) with pea protein and could form five hydrophobic bonds and four hydrogen bonds with pea protein. These findings would provide a new idea for the masking of bitterness and the evolution of Pickering emulsions stabilised by the protein–polyphenol nanoparticles.
Combined effects of paper‐containing 1‐methylcyclopropene with nitrogen‐modified atmosphere packaging in the logistics process of e‐commerce on fruit quality of plum (Prunus salicina cv. ‘Qingcuili’) during shelf life
Authors: Zhang Qianqian , Guan Wenqiang , Xiong Jinliang , Lin Hengxun , Wang Xueqing , Huang Ming , Tan Junjie , Lei Guisheng , Luo Qingyi , Zhao Qian 
Published: May 2023

SummaryIn this study, the paper‐containing 1‐methylcyclopropene (1‐MCP) and 100% nitrogen (modified atmosphere packaging, MAP) were used to treat plums during logistics process of e‐commerce (about 24 h). Quality changes of plums were analysed during shelf life at 25 °C and 4 °C for 6 and 12 days, respectively. Results showed that compared with control, 1‐MCP, MAP and 1‐MCP + MAP decreased respiration rate and ethylene production, maintained lower weight loss, higher green (a*) and firmness, maintained titratable acidity (TA) and ascorbic acid (ASA) content, reduced malondialdehyde (MDA) accumulation, inhibited polyphenol oxidase (PPO) activity and maintained peroxidase (POD) activity. The quality of 1‐MCP + MAP group was the best, followed by the 1‐MCP group and MAP group. Overall, the combination of 1‐MCP with MAP treatments in the logistics process of e‐commerce has potential prospects for application to maintain the shelf life quality of fruits.
Comparison of different pretreatments on drying characteristics, physical properties, nutritional compounds and antioxidant activity of heat pump‐dried longan pulp
Authors: Zhu Jingnan , Peng Jian , Li Jun , Li Lu , Bi Jinfeng , Yang Wanyuan , Yi Jianyong , Chen Shupeng , Xu Yujuan , Gu Qingqing 
Published: May 2023

SummaryDrying pretreatments are usually considered as effective way to improve the quality of dried fruit products. The results of this research showed that the blanching (BL), ultrasonic (US) and freeze–thaw (FT) pretreatments could significantly shorten the drying time, and improve the rehydration capacity of the dried longan pulp. Compared with the control group (CK, without pretreatment), more irregular inner micropores were formed in the dried longan pulp treated with BL, US and FT. Besides, the brightness and yellowness of dried longan pulp with pretreatments were higher than those of the CK. The higher retention rates of total phenolic content (292.54 mg/100 g d.b.) and ascorbic acid (2.38 mg g−1 d.b.) were observed in the dried longan pulp treated with BL. Meanwhile, a higher chlorogenic acid content was found in the samples treated with FT (3.68 mg/100 g d.b.) and radio frequency (RF) (3.10 mg/100 g d.b.). Furthermore, the highest retention of a total free amino acid (1843.13 mg/100 g d.b) was exhibited by RF‐treated longan pulp. This work guides how pretreatments are applied prior to longan pulp drying.
Determinants of the success in flap reconstruction—Outcome analysis of 120 flaps in 484 procedures for pressure injury
Authors: Huang Ching‐Ya , Lee Sheng‐Lian , Chiu Wen‐Kuan , Chen Chiehfeng , Chen Jin‐Hua , Wang Hsian‐Jenn 
Published: May 2023

AbstractPressure injury (PI) mainly occurs in bedridden older adults or those with physical limitations. Here, we aimed to determine the appropriate timing to conduct flap reconstruction in patients with PIs and identify factors affecting surgical outcomes. We retrospectively reviewed the data of all patients who received debridement or flap reconstruction surgery for PIs in our hospital from January 2016 to December 2021. The extracted data included patient demographics, surgical records, blood test results, vital signs, and flap outcomes. In total, 484 surgical procedures (364 debridements and 120 flaps) were performed on 216 patients. Serum albumin level of ≥2.5 g/dL remarkably increased the likelihood of complete wound healing (odds ratio [OR] = 4.12, P = .032) and reduced the risk of postoperative complications (OR = 0.26, P = .040). In contrast, advanced age (OR = 1.04, P = .045) and serum creatinine level ≥2 mg/dL (OR = 5.07, P = .016) increased the risk of postoperative complications. Thus, patients with a favourable nutrition status have a higher likelihood of achieving complete wound healing. By contrast, patients who are older and have serum creatinine ≥2 mg/dL and serum albumin <2.5 g/dL tend to develop more postoperative complications. Overall, thorough correction for patient inflammation, infection, anaemia, and malnutrition status can provide optimal flap surgery outcomes.
Surgical site infection in skin surgery—An observational study
Authors: Schlager Justin Gabriel , Patzer Kathrin , Wallmichrath Jens , French Lars E. , Kunrad Elena , Schlingmann Sophia , Stiefel Daniel , Kendziora Benjamin , Hartmann Daniela 
Published: May 2023

AbstractBackgroundSurgical site infection (SSI) has a significant impact on patients’ morbidity and aesthetic results.ObjectiveTo identify risk factors for SSI in dermatologic surgery.Patients and MethodsThis prospective, single‐centre, observational study was performed between August 2020 and May 2021. Patients that presented for dermatologic surgery were included and monitored for the occurrence of SSI. For statistical analysis, we used a mixed effects logistic regression model.ResultsOverall, 767 patients with 1272 surgical wounds were included in the analysis. The incidence of SSI was 6.1%. Significant risk factors for wound infection were defect size over 10cm2 (OR 3.64, 95% confidence interval [CI] 1.80–7.35), surgery of cutaneous malignancy (OR 2.96, CI 1.41–6.24), postoperative bleeding (OR 4.63, CI 1.58–13.53), delayed defect closure by local skin flap (OR 2.67, CI 1.13–6.34) and localisation of surgery to the ear (OR 7.75, CI 2.07–28.99). Wound localisation in the lower extremities showed a trend towards significance (OR 3.16, CI 0.90–11.09). Patient‐related factors, such as gender, age, diabetes, or immunosuppression, did not show a statistically significant association with postoperative infection.ConclusionLarge defects, surgery of cutaneous malignancy, postoperative bleeding, and delayed flap closure increase the risk for SSI. High‐risk locations are the ears and lower extremities.
Bifidobacterium breve CBT BR3 is effective at relieving intestinal inflammation by augmenting goblet cell regeneration
Authors: Park I Seul , Kim Ji Hyung , Yu Jongwook , Shin YooJin , Kim Kibeom , Kim Tae Il , Kim Seung Won , Cheon Jae Hee 
Published: May 2023

AbstractBackground and AimBifidobacterium breve was the first bacteria isolated in the feces of healthy infants and is a dominant species in the guts of breast‐fed infants. Some strains of B. breve have been shown to be effective at relieving intestinal inflammation, but the modes of action have yet to be elucidated. In this study, we investigated the mechanisms of action of B. breve CBT BR3 isolated from South Korean infant feces in relieving colitis in vitro and in vivo.MethodsColitis was induced in mice with dextran sodium sulfate (DSS) and dinitrobenzene sulfonic acid (DNBS). Quantitative reverse‐transcription polymerase chain reaction, in vitro FITC‐dextran flux permeability assay, and aryl hydrocarbon receptor (AhR) luciferase assay are performed using Caco‐2 cells and HT29‐Lucia™ AhR cells.ResultsB. breve CBT BR3 was orally administered. B. breve CBT BR3 improved colitis symptoms in both DSS‐ and DNBS‐induced colitis models. B. breve CBT BR3 increased the number of goblet cells per crypt. B. breve increased the mRNA expressions of Notch, Spdef, Muc5, and Il22. The mRNA expressions of Occludin, which encodes a membrane tight‐junction protein, and Foxo3, which encodes a protein related to butyrate metabolism, were also increased in the DSS‐ and DNBS‐induced colitis models. B. breve CBT BR3 protected inflammation‐induced epithelial cell permeability and improved goblet cell function by inducing aryl hydrocarbon receptor in vitro.ConclusionsThese results indicate that B. breve CBT BR3 is effective at relieving intestinal inflammation by augmenting goblet cell regeneration.