bluuebunny/crossref_metadata_2025_split huggingface dataset:
Hemispheric Asymmetry of the Polar Ionospheric Density Investigated by ESR and JVD Radar Observations and TIEGCM Simulations for the Solar Minimum Period
Authors: Kim E. , Jee G. , Wang W. , Kwak Y.‐S. , Shim J.‐S. , Ham Y.‐B. , Kim Y. H. 
Published: January 2023

AbstractThe ionospheric density displays hemispheric asymmetries in the polar region due to various hemispheric differences, for example, in the offset between geographic and geomagnetic poles and in the geomagnetic field strength. Using ground‐based ionospheric measurements from Vertical Incidence Pulsed Ionospheric Radar with Dynasonde analysis at Jang Bogo Station (JBS), Antarctica and from EISCAT Svalbard Radar (ESR) where both sites are located mostly in the polar cap, we investigate the hemispheric differences in the ionospheric density between the northern and southern hemispheres for geomagnetically quiet and solar minimum condition. The results are also compared with Thermosphere Ionosphere Electrodynamic Global Circulation Model (TIEGCM) simulations. The observations show larger density and stronger diurnal and seasonal variations at JBS in the southern hemisphere than at Svalbard in the northern hemisphere. The diurnal variations of the density peak height are also observed to be much larger at JBS. In both hemispheres, the ionospheric density is significantly reduced in winter due to the limited solar production at high geographic latitudes, but TIEGCM considerably overestimates winter density, which is even larger than summer density, especially in the northern hemisphere. Also existed are the differences in the equinoctial asymmetry between the observations and the simulations: the daytime F‐region density is observed to be larger in fall than in spring in both hemispheres, but TIEGCM shows the opposite. In general, most of the observed asymmetrical density are much weaker in the model simulation, which may result from lack of proper magnetospheric forcings and neutral dynamics in the model.
Forced Subduction Initiation Near Spreading Centers: Effects of Brittle‐Ductile Damage
Authors: Liu Mingqi , Gerya Taras 
Published: January 2023

AbstractAlthough positive buoyancy of young lithosphere near spreading centers does not favor spontaneous subduction, subduction initiation occurs easily near ridges due to their intrinsic rheological weakness when plate motion reverses from extension to compression. It has also been repeatedly proposed that inherited detachment faults may directly control the nucleation of new subduction zones near ridges subjected to forced compression. However, recent 3D numerical experiments suggested that direct inversion of a single detachment fault does not occur. Here we further investigate this controversy numerically by focusing on the influence of brittle‐ductile damage on the dynamics of near‐ridge subduction initiation. We self‐consistently model the inversion of tectonic patterns formed during oceanic spreading using 3D high‐resolution thermomechanical numerical models with strain weakening of faults and grain size evolution. Numerical results show that forced compression predominantly reactivates and rotates inherited extensional faults, shortening and thickening the weakest near‐ridge region of the oceanic lithosphere, thereby producing ridge swellings. As a result, a new megathrust zone is developed, which accommodates further shortening and subduction initiation. Furthermore, brittle/plastic strain weakening has a key impact on the collapse of the thickened ridge and the onset of near‐ridge subduction initiation. In contrast, grain size evolution of the mantle only slightly enhances the localization of shear zones at the brittle‐ductile transition and thus plays a subordinate role. Compared to the geological record, our numerical results provide new helpful insights into possible physical controls and dynamics of natural near‐ridge subduction initiation processes recorded by the Mirdita ophiolite of Albania.
The Initiation of Frictional Motion—The Nucleation Dynamics of Frictional Ruptures
Authors: Gvirtzman Shahar , Fineberg Jay 
Published: January 2023

AbstractFrictional interfaces lose stability via earthquake‐like ruptures, which are close analogues of shear cracks that are well‐described by fracture mechanics. Interface ruptures, however, need to be first formed—or nucleated. Rupture nucleation therefore determines the onset of friction, replacing the concept of a characteristic “static friction coefficient”. Utilizing rupture arrest at an imposed barrier, we experimentally determine nucleation locations, times and stresses at the origin of each subsequent rupture event. This enables us study the nucleation process via real‐time measurements of real contact area and local strain. Nucleation events initiate as 2D patches that expand at nearly constant velocities, vnuc, that are orders of magnitude lower than the dynamic rupture velocities described by conventional fracture mechanics. We find that: (a) Nucleation has location‐dependent stress thresholds, (b) vnuc is roughly proportional to the local stress level, (c) the nucleation process continues until the patch size reaches Ltran ∼ LG, the Griffith length for the onset of dynamic fracture (d) scaling time by τ = Ltran/vnuc, nucleation patches exhibit self‐similar dynamics (e) dynamic ruptures' cohesive zones are not fully established until significantly beyond Ltran. Many details of nucleation are governed by the local contact area topography, which is roughly invariant under successive rupture events in mature interfaces. Topography‐dependent details of the nucleation process include: precise nucleation site location, patch geometry, critical stress thresholds and the proportionality constant of vnuc with stress. We believe that these results shed considerable light on both how frictional motion is triggered and earthquake initiation.
Prion infection modulates hematopoietic stem/progenitor cell fate through cell-autonomous and non-autonomous mechanisms
Authors: Sim Hyun-Jaung , Kim Yong-Chan , Bhattarai Govinda , Won Sae-Young , Lee Jeong-Chae , Jeong Byung-Hoon , Kook Sung-Ho 
Published: January 2023

AbstractStudies of PrPC-derived prion disease generally focus on neurodegeneration. However, little is known regarding the modulation of hematopoietic stem progenitor cells (HSPCs) that express PrPC in prion infection. Among bone marrow (BM) hematopoietic cells, hematopoietic stem cells (HSCs) strongly express PrPC. A bioassay revealed the presence of misfolded prion protein (PrPSc) in BM cells derived from prion-infected mice; these BM cells demonstrated reproducible prion infectivity. At 5 months after infection with ME7, mice exhibited a significant decrease in the number of HSPCs. This decrease was mainly driven by increased apoptotic cell death, rather than cell cycle progression and senescence, in PrPC-positive but not PrPC-negative HSPC populations through a cell-autonomous mechanism. Notably, both PrPC-positive and PrPC-negative HSCs underwent cellular senescence, as indicated by high levels of senescence-associated factors and deficits in repopulation and self-renewal capacities at 7 months after infection. Senescence of HSCs occurred in the ME7-impaired BM microenvironment with aging phenotypes through non-cell autonomous mechanisms. These data provide novel evidence that prion infection differentially modulates HSC fate through both cell-autonomous and non-autonomous mechanisms.
Impact of retrotransposon protein L1 ORF1p expression on oncogenic pathways in hepatocellular carcinoma: the role of cytoplasmic PIN1 upregulation
Authors: Zadran Bassier , Sudhindar Praveen Dhondurao , Wainwright Daniel , Bury Yvonne , Luli Saimir , Howarth Rachel , McCain Misti Vanette , Watson Robyn , Huet Hannah , Palinkas Fanni , Berlinguer-Palmini Rolando , Casement John , Mann Derek A. , Oakley Fiona , Lunec John , Reeves Helen , Faulkner Geoffrey J. , Shukla Ruchi 
Published: January 2023

Abstract
Background
Molecular characterisation of hepatocellular carcinoma (HCC) is central to the development of novel therapeutic strategies for the disease. We have previously demonstrated mutagenic consequences of Long-Interspersed Nuclear Element-1 (LINE1s/L1) retrotransposition. However, the role of L1 in HCC, besides somatic mutagenesis, is not well understood.

Methods
We analysed L1 expression in the TCGA-HCC RNAseq dataset (n = 372) and explored potential relationships between L1 expression and clinical features. The findings were confirmed by immunohistochemical (IHC) analysis of an independent human HCC cohort (n = 48) and functional mechanisms explored using in vitro and in vivo model systems.

Results
We observed positive associations between L1 and activated TGFβ-signalling, TP53 mutation, alpha-fetoprotein and tumour invasion. IHC confirmed a positive association between pSMAD3, a surrogate for TGFβ-signalling status, and L1 ORF1p (P < 0.0001, n = 32). Experimental modulation of L1 ORF1p levels revealed an influence of L1 ORF1p on key hepatocarcinogenesis-related pathways. Reduction in cell migration and invasive capacity was observed upon L1 ORF1 knockdown, both in vitro and in vivo. In particular, L1 ORF1p increased PIN1 cytoplasmic localisation. Blocking PIN1 activity abrogated L1 ORF1p-induced NF-κB-mediated inflammatory response genes while further activated TGFβ-signalling confirming differential alteration of PIN1 activity in cellular compartments by L1 ORF1p.

Discussion
Our data demonstrate a causal link between L1 ORF1p and key oncogenic pathways mediated by PIN1, presenting a novel therapeutic avenue.
USP39 stabilizes β-catenin by deubiquitination and suppressing E3 ligase TRIM26 pre-mRNA maturation to promote HCC progression
Authors: Wang Weiwei , Lei Yongbin , Zhang Gongye , Li Xiaomei , Yuan Jiahui , Li Tingting , Zhong Wei , Zhang Yuqi , Tan Xuemei , Song Gang 
Published: January 2023

AbstractUbiquitin-specific protease 39(USP39) plays an important role in modulating pre-mRNA splicing and ubiquitin-proteasome dependent proteolysis as a member of conserved deubiquitylation family. Accumulating evidences prove that USP39 participates in the development of hepatocellular carcinoma (HCC). However, little is known about the mechanism especially deubiquitinating target of USP39 in regulating hepatocellular carcinoma (HCC) growth. Here, we prove that USP39 promotes HCC cell proliferation and migration by directly deubiquitin β-catenin, a key molecular of Wnt/β-catenin signaling pathway whose abnormal expression or activation results in several tumors, following its co-localization with USP39. In this process, the expression of E3 ligase TRIM26, which is proved to restrain HCC in our previous research, shows a decreasing trend. We further demonstrate that TRIM26 pre-mRNA splicing and maturation is inhibited by USP39, accompanied by its reduction of ubiquitinating β-catenin, facilitating HCC progression indirectly. In summary, our data reveal a novel mechanism in the progress of HCC that USP39 promotes the proliferation and migration of HCC through increasing β-catenin level via both direct deubiquitination and reducing TRIM26 pre-mRNA maturation and splicing, which may provide a new idea and target for clinical treatment of HCC.
Computer vision quantification of whole-body Parkinsonian bradykinesia using a large multi-site population
Authors: Morinan Gareth , Dushin Yuriy , Sarapata Grzegorz , Rupprechter Samuel , Peng Yuwei , Girges Christine , Salazar Maricel , Milabo Catherine , Sibley Krista , Foltynie Thomas , Cociasu Ioana , Ricciardi Lucia , Baig Fahd , Morgante Francesca , Leyland Louise-Ann , Weil Rimona S. , Gilron Ro’ee , O’Keeffe Jonathan 
Published: January 2023

AbstractParkinson’s disease (PD) is a common neurological disorder, with bradykinesia being one of its cardinal features. Objective quantification of bradykinesia using computer vision has the potential to standardise decision-making, for patient treatment and clinical trials, while facilitating remote assessment. We utilised a dataset of part-3 MDS-UPDRS motor assessments, collected at four independent clinical and one research sites on two continents, to build computer-vision-based models capable of inferring the correct severity rating robustly and consistently across all identifiable subgroups of patients. These results contrast with previous work limited by small sample sizes and small numbers of sites. Our bradykinesia estimation corresponded well with clinician ratings (interclass correlation 0.74). This agreement was consistent across four clinical sites. This result demonstrates how such technology can be successfully deployed into existing clinical workflows, with consumer-grade smartphone or tablet devices, adding minimal equipment cost and time.
Quantitative assessment method of muzzle flash and smoke at high noise level on field environment
Authors: Yan Chenguang , Zhu Chenguang 
Published: January 2023

AbstractIt is quite a challenge to obtain the temperature and species concentration fields of muzzle flash at high noise level. In this numerical study, radiation intensity of muzzle flash received by the high-speed Complementary Metal-Oxide-Semiconductor (CMOS) camera was simulated based on the line-of-sight method in the direct radiative transfer problem. The inverse radiative transfer problem of reconstructing distributions of temperature and soot volume fraction from the knowledge of flame radiation intensity was transformed into a minimization optimization problem and a meta-heuristic algorithm was used to solve the problem. The effects of the number of detection lines, optical thickness and measurement errors on the reconstruction results were discussed in details. A method to estimate the noise level of radiation intensity was developed, experimental results showed that the signal-to-noise ratio (SNR) of radiation intensity can be successfully inferred when the SNR is greater than 20 dB. Subsequently, prior knowledge of the noise level was introduced in the regularization to achieve a meaningful approximation of the exact value. The reconstruction of the soot volume fraction filed with SNR greater than 40 dB is considered successful with the inclusion of an appropriate regularization term in the objective function, and the reconstruction of the temperature field is feasible even with SNR as low as 15 dB. The high tolerance to the noise level of the radiation intensity gives the reconstruction algorithm the potential to be used in practical experiments of muzzle flash.
TLR4 and MD2 variation among horses with differential TNFα baseline concentrations and response to intravenous lipopolysaccharide infusion
Authors: Mukhopadhyay Abhijit , Cook Shawna R. , SanMiguel Phillip , Ekenstedt Kari J. , Taylor Sandra D. 
Published: January 2023

AbstractGram-negative bacterial septicemia is mediated through binding of lipopolysaccharide (LPS) to mammalian toll-like receptor protein 4 (TLR4). TLR4 and its cognate protein, myeloid differentiation factor 2 (MD2) form a heterodimeric complex after binding LPS. This complex induces a cascade of reactions that results in increased proinflammatory cytokine gene expression, including TNFα, which leads to activation of innate immunity. In horses, the immune response to LPS varies widely. To determine if this variation is due to differences in TLR4 or MD2, DNA from 15 healthy adult horses with different TNFα dynamics after experimental intravenous LPS infusion was sequenced across exons ofTLR4andMD2. Haplotypes were constructed for both genes using all identified variants. Four haplotypes were observed for each gene. No significant associations were found between either TNFα baseline concentrations or response to LPS and haplotype; however, there was a significant association (Pvalue = 0.0460) between the baseline TNFα concentration and oneMD2missense variant. Three-dimensional structures of the equine TLR4-MD2-LPS complex were built according to haplotype combinations observed in the study horses, and the implications of missense variants on LPS binding were modeled. Although the sample size was small, there was no evidence that variation in TLR4 or MD2 explains the variability in TNFα response observed after LPS exposure in horses.
Inferring genetic structure when there is little: population genetics versus genomics of the threatened bat Miniopterus schreibersii across Europe
Authors: Dufresnes Christophe , Dutoit Ludovic , Brelsford Alan , Goldstein-Witsenburg Fardo , Clément Laura , López-Baucells Adria , Palmeirim Jorge , Pavlinić Igor , Scaravelli Dino , Ševčík Martin , Christe Philippe , Goudet Jérôme 
Published: January 2023

AbstractDespite their paramount importance in molecular ecology and conservation, genetic diversity and structure remain challenging to quantify with traditional genotyping methods. Next-generation sequencing holds great promises, but this has not been properly tested in highly mobile species. In this article, we compared microsatellite and RAD-sequencing (RAD-seq) analyses to investigate population structure in the declining bent-winged bat (Miniopterus schreibersii) across Europe. Both markers retrieved general patterns of weak range-wide differentiation, little sex-biased dispersal, and strong isolation by distance that associated with significant genetic structure between the three Mediterranean Peninsulas, which could have acted as glacial refugia. Microsatellites proved uninformative in individual-based analyses, but the resolution offered by genomic SNPs illuminated on regional substructures within several countries, with colonies sharing migrators of distinct ancestry without admixture. This finding is consistent with a marked philopatry and spatial partitioning between mating and rearing grounds in the species, which was suspected from marked-recaptured data. Our study advocates that genomic data are necessary to properly unveil the genetic footprints left by biogeographic processes and social organization in long-distant flyers, which are otherwise rapidly blurred by their high levels of gene flow.
Continuous intraoperative perfusion monitoring of free microvascular anastomosed fasciocutaneous flaps using remote photoplethysmography
Authors: Schraven Sebastian P. , Kossack Benjamin , Strüder Daniel , Jung Maximillian , Skopnik Lotte , Gross Justus , Hilsmann Anna , Eisert Peter , Mlynski Robert , Wisotzky Eric L. 
Published: January 2023

AbstractFlap loss through limited perfusion remains a major complication in reconstructive surgery. Continuous monitoring of perfusion will facilitate early detection of insufficient perfusion. Remote or imaging photoplethysmography (rPPG/iPPG) as a non-contact, non-ionizing, and non-invasive monitoring technique provides objective and reproducible information on physiological parameters. The aim of this study is to establish rPPG for intra- and postoperative monitoring of flap perfusion in patients undergoing reconstruction with free fasciocutaneous flaps (FFCF). We developed a monitoring algorithm for flap perfusion, which was evaluated in 15 patients. For 14 patients, ischemia of the FFCF in the forearm and successful reperfusion of the implanted FFCF was quantified based on the local signal. One FFCF showed no perfusion after reperfusion and devitalized in the course. Intraoperative monitoring of perfusion with rPPG provides objective and reproducible results. Therefore, rPPG is a promising technology for standard flap perfusion monitoring on low costs without the need for additional monitoring devices.
Synthesis and characterization of linear/nonlinear optical properties of graphene oxide and reduced graphene oxide-based zinc oxide nanocomposite
Authors: Ebrahimi Naghani Mohsen , Neghabi Mina , Zadsar Mehdi , Abbastabar Ahangar Hossein 
Published: January 2023

AbstractIn this paper, we aimed to investigate the linear and nonlinear optical properties of GO-ZnO and RGO-ZnO nanocomposites in comparison with pure GO and reduced graphene oxide (RGO). For this purpose, GO, RGO, GO-ZnO, and RGO-ZnO were synthesized and characterized by Fourier transform infrared (FT-IR), Ultraviolet–Visible (UV–Vis) absorption, X-ray diffraction (XRD) and energy dispersive X-ray spectroscopy (EDX). XRD and EDX analysis indicated the reduction of GO as well as the successful synthesis of GO-ZnO and RGO-ZnO nanocomposites. The FT-IR spectroscopy showed that absorption bands were at 3340 cm−1, 1630 cm−1, 1730 cm−1 and 480 cm−1 related to OH, C=C, C=O, and Zn–O stretching vibrations, respectively. The direct band gaps of GO, RGO, GO-ZnO and RGO-ZnO from UV–Vis spectra were at 3.36, 3.18, 3.63 and 3.25 eV, sequentially. Moreover, the third-order nonlinear optical properties were investigated using a z-scan technique with Nd: YAG laser (532 nm, 70 mW). It can be seen that the nonlinear absorption coefficient value $$(\upbeta )$$

(
β
)

increased from 5.3 × 10–4 (GO) to 8.4 × 10–3 cm/W (RGO-ZnO). In addition, nonlinear refractive index (n2) of the GO, RGO, GO-ZnO, and RGO-ZnO was obtained as 10.9 × 10–10, 14.3 × 10–10, 22.9 × 10–10, and 31.9 × 10–10 cm2/W respectively.
The impact of pregnancy and childbirth on pelvic sensation: a prospective cohort study
Authors: Mahoney Charlotte K. , Reid Fiona M. , Smith Anthony R. B. , Myers Jenny E. 
Published: January 2023

AbstractPelvic organ prolapse, urinary, bowel and sexual dysfunction, collectively called pelvic floor dysfunction (PFD) affects 1 in 3 women and has a significant public health impact. The causes of PFD are not fully understood but involve injury to connective tissue and motor nerve during childbirth. Women with PFD also have sensory nerve impairment, and it is likely this occurs during childbirth, but this has never been investigated. In the current study 150 women underwent quantitative sensory testing for vibration sensation at the vagina and clitoris, and stretch sensation at the vagina and introitus, in the third trimester, 3 and 6 months postnatal. Antenatally vibration sensation was reduced but stretch sensation was normal. Postnatally vibration sensation deteriorated whilst stretch sensation initially deteriorated but recovered by 6 months postnatal to antenatal levels (all p < 0.001). Mode of birth had a significant impact on sensation, with caesarean section appearing neuroprotective, normal vaginal birth resulted in a transient deterioration in sensation that recovered by 6 months, whilst assisted vaginal delivery was prolonged suggesting persistent neurological impairment (all p < 0.015). Further research is required to study the clinical effect of these changes on pelvic floor dysfunction in the medium and long-term.