bluuebunny/crossref_metadata_2025_split huggingface dataset:
A chromosome scale assembly of the tarnished plant bug, Lygus lineolaris (Palisot de Beauvois), genome
Authors: Perera O. P. , Saha Surya , Glover James , Parys Katherine A. , Allen K. Clint , Grozeva Snejana , Kurtz Ryan , Reddy Gadi V. P. , Johnston J. Spencer , Daly Mark , Swale Thomas 
Published: June 2023

Abstract
Objective
The tarnished plant bug (TPB), Lygus lineolaris (Palisot de Beauvois) (Hemiptera: Miridae), is a pest damaging many cultivated crops in North America. Although partial transcriptome data are available for this pest, a genome assembly was not available for this species. This assembly of a high-quality chromosome-length genome of TPB is aimed to develop the genetic resources that can provide the foundation required for advancing research on this species.

Results
The initial genome of TPB assembled with paired-end nucleotide sequences generated with Illumina technology was scaffolded with Illumina HiseqX reads generated from a proximity ligated (HiC) library to obtain a high-quality genome assembly. The final assembly contained 3963 scaffolds longer than 1 kbp to yield a genome of 599.96 Mbp. The N50 of the TPB genome assembly was 35.64 Mbp and 98.68% of the genome was assembled into 17 scaffolds larger than 1 Mbp. This megabase scaffold number is the same as the number of chromosomes observed in karyotyping of this insect. The TPB genome is known to have high repetitive DNA content, and the reduced assembled genome size compared to flowcytometric estimates of approximately 860 Mbp may be due to the collapsed assembly of highly similar regions.
Polyetheretherketone implants with hierarchical porous structure for boosted osseointegration
Authors: Chen Zhiyong , Chen Yu , Wang Yang , Deng JiaJia , Wang Xin , Wang Qingqing , Liu Yuehua , Ding Jiandong , Yu Lin 
Published: June 2023

Abstract

Background
Good osseointegration is the key to the long-term stability of bone implants. Thermoplastic polyetheretherketone (PEEK) has been widely used in orthopedics; however, its inherent biological inertia causes fibrous tissue to wrap its surface, which leads to poor osseointegration and thus greatly limits its clinical applications.

Methods
Herein, we developed a facile yet effective surface modification strategy. A commonly used sulfonation coupled with “cold pressing” treatment in the presence of porogenic agent formed a three-dimensional hierarchical porous structure on PEEK surface. Subsequently, the effects of porous surface on the in vitro adhesion, proliferation and differentiation of rat bone marrow-derived mesenchymal stem cells (BMSCs) were evaluated. Finally, the osteoinduction and osseointegration of surface-porous PEEK implant were examined in the rat distal femoral defect model.

Results
In vitro results showed that the surface modification did not significantly affect the mechanical performance and cytocompatibility of PEEK substance, and the porous structure on the modified PEEK substrate provided space for cellular ingrowth and enhanced osteogenic differentiation and mineralization of BMSCs. In vivo tests demonstrated that the surface-porous PEEK implant could effectively promote new bone formation and had higher bone-implant contact rate, thereby achieving good bone integration with the surrounding host bone. In addition, this modification technique was also successfully demonstrated on a medical PEEK interbody fusion cage.

Conclusion
The present study indicates that topological morphology plays a pivotal role in determining implant osseointegration and this facile and effective modification strategy developed by us is expected to achieve practical applications quickly.

Graphical Abstract
Hybrid control using fuzzy logic and adaptive space vector modulation for reduction of torque ripples in PM-BLDC motor drive
Authors: Karan Vijay Kumar , Alam Aftab , Thakur Amarnath 
Published: June 2023

AbstractA novel hybrid control strategy for reducing torque ripple in permanent magnet brushless direct current motors has been developed by combining the deterministic nature of direct torque control and the adaptive nature of fuzzy logic control. Direct torque control using space vector pulse width modulation has been developed. This technique ensures the near-sinusoidal nature of the stator and rotor current. The impact of commutation ripple corresponding to the sinusoidal current is reduced compared to square wave current in conventional direct torque control. Further, the fuzzy logic controller provides adaptive modulation in space vector to mitigate the torque ripple. The response of the proposed method has been analyzed in the time domain as well as in the frequency domain. Experimental results establish a significant reduction in torque ripple and minimization of high-frequency components. A comparative study with conventional direct torque control methods has also been presented.
Pleiotropy of autism-associated chromatin regulators
Authors: Lasser Micaela , Sun Nawei , Xu Yuxiao , Wang Sheng , Drake Sam , Law Karen , Gonzalez Silvano , Wang Belinda , Drury Vanessa , Castillo Octavio , Zaltsman Yefim , Dea Jeanselle , Bader Ethel , McCluskey Kate E. , State Matthew W. , Willsey A. Jeremy , Willsey Helen Rankin 
Published: June 2023

ABSTRACT
Gene ontology analyses of high-confidence autism spectrum disorder (ASD) risk genes highlight chromatin regulation and synaptic function as major contributors to pathobiology. Our recent functional work in vivo has additionally implicated tubulin biology and cellular proliferation. As many chromatin regulators, including the ASD risk genes ADNP and CHD3, are known to directly regulate both tubulins and histones, we studied the five chromatin regulators most strongly associated with ASD (ADNP, CHD8, CHD2, POGZ and KMT5B) specifically with respect to tubulin biology. We observe that all five localize to microtubules of the mitotic spindle in vitro in human cells and in vivo in Xenopus. Investigation of CHD2 provides evidence that mutations present in individuals with ASD cause a range of microtubule-related phenotypes, including disrupted localization of the protein at mitotic spindles, cell cycle stalling, DNA damage and cell death. Lastly, we observe that ASD genetic risk is significantly enriched among tubulin-associated proteins, suggesting broader relevance. Together, these results provide additional evidence that the role of tubulin biology and cellular proliferation in ASD warrants further investigation and highlight the pitfalls of relying solely on annotated gene functions in the search for pathological mechanisms.
Phosphorylation of Rab29 at Ser185 regulates its localization and role in the lysosomal stress response in concert with LRRK2
Authors: Komori Tadayuki , Kuwahara Tomoki , Fujimoto Tetta , Sakurai Maria , Koyama-Honda Ikuko , Fukuda Mitsunori , Iwatsubo Takeshi 
Published: June 2023

ABSTRACT
Rab proteins are small GTPases that regulate a myriad of intracellular membrane trafficking events. Rab29 is one of the Rab proteins phosphorylated by leucine-rich repeat kinase 2 (LRRK2), a Parkinson's disease-associated kinase. Recent studies suggest that Rab29 regulates LRRK2, whereas the mechanism by which Rab29 is regulated remained unclear. Here, we report a novel phosphorylation in Rab29 that is not mediated by LRRK2 and occurs under lysosomal overload stress. Mass spectrometry analysis identified the phosphorylation site of Rab29 as Ser185, and cellular expression studies of phosphomimetic mutants of Rab29 at Ser185 unveiled the involvement of this phosphorylation in counteracting lysosomal enlargement. PKCα and PKCδ were deemed to be involved in this phosphorylation and control the lysosomal localization of Rab29 in concert with LRRK2. These results implicate PKCs in the lysosomal stress response pathway comprised of Rab29 and LRRK2, and further underscore the importance of this pathway in the mechanisms underlying lysosomal homeostasis.
Comparative evaluation of clinical performance, child and parental satisfaction of Bioflex, zirconia and stainless steel crowns in pediatric patients
Authors: Rahate Ishani , Fulzele Punit , Thosar Nilima 
Published: June 2023

Background: Pediatric treatment is challenging in patients with early childhood caries. It is difficult due to the adjacency of pulp, miniature tooth size, and thin enamel compared to permanent dentition. Nowadays, aesthetics play an important role in managing decayed teeth, and children need treatment that includes full coverage crowns in either stainless steel or anesthetic crown in zirconia or the recently developed Bioflex crown. The Bioflex crowns are highly flexible and aesthetically preformed pediatric crowns with combined stainless steel and zirconia properties. This study aims to assess the clinical performance and child and parental satisfaction of Bioflex crowns compared to zirconia and stainless steel crowns. Methods: In the current in-vivo comparison of Bioflex crowns with zirconia and stainless steel crowns, children aged three to seven years old will be selected, and 72 primary teeth requiring crowns will be randomly distributed into three groups, n = 24: Group I: Preformed stainless steel crown, control; Group II: Preformed Bioflex crown; Group III: Preformed zirconia crown. Crowns will be evaluated for recurrent caries, plaque accumulation, restoration failure, gingival status opposing tooth wear, and clinicians and parental satisfaction at zero, three, six, and 12 months. Newly introduced aesthetic crowns will serve as a versatile alternative for restoring primary decayed teeth that over-performed aesthetic and conventional crowns. Conclusions: The Bioflex crown will be assessed as a better aesthetic substitute for the future, and the satisfaction level of parents will be evaluated. Trial registration: CTRI registration number: CTRI/2023/05/052256; Date of registration: May 03, 2023. Protocol version: Two; Date: April 22, 2023
Creative Destruction? Impact of E-Commerce on the Retail Sector
Authors: Chava Sudheer , Oettl Alexander , Singh Manpreet , Zeng Linghang 
Published: June 2023

Using an administrative payroll data set for 2.6 million retail workers, we find that the staggered rollout of a major e-commerce firm’s fulfillment centers reduces traditional retail workers’ income in geographically proximate counties by 2.4%. Wages of hourly workers, especially part-time hourly workers, decrease significantly driven by a drop in the number of hours worked. We observe a U-shaped pattern in which both young and old workers experience a sharper decrease in wage income. Consequently, some workers experience an increase in credit card delinquency. Using data for 3.2 million stores, we find that sales (employment) at proximate stores decrease by 4% (2.1%). Exits, especially of young and small stores, increase, and entry decreases. Our results highlight how creative destruction led by e-commerce impacts local labor markets. This paper was accepted by Anindya Ghose, information systems. Funding: The authors gratefully acknowledge funding support from the Kauffman Foundation Junior Faculty Fellowship. Supplemental Material: The online appendix and data are available at https://doi.org/10.1287/mnsc.2023.4795 .
Punish Underperformance with Suspension: Optimal Dynamic Contracts in the Presence of Switching Cost
Authors: Cao Ping , Sun Peng , Tian Feng 
Published: June 2023

This paper studies a dynamic principal–agent setting in which the principal needs to dynamically schedule an agent to work or be suspended. When the agent is directed to work and exert effort, the arrival rate of a Poisson process is increased, which increases the principal’s payoff. Suspension, on the other hand, serves as a threat to the agent by delaying future payments. A key feature of our setting is a switching cost whenever the suspension stops and the work starts again. We formulate the problem as an optimal control model with switching and fully characterize the optimal control policies/contract structures under different parameter settings. Our analysis shows that, when the switching cost is not too high, the optimal contract demonstrates a generalized control-band structure. The length of each suspension episode, on the other hand, is fixed. Overall, the optimal contract is easy to describe, compute, and implement. This paper was accepted by Ilia Tsetlin, behavioral economics and decision analysis. Funding: The work of P. Cao is financially supported by the National Natural Science Foundation of China [Grants 72122019 and 71771202]. The work of F. Tian is financially supported by the Hong Kong Research Grants Council (RGC) funding (RGC Early Career Scheme 2022/23) [Grant 27500822]. Supplemental Material: The e-companion and data are available at https://doi.org/10.1287/mnsc.2023.4845 .
Leukocyte differentiation in bronchoalveolar lavage fluids using higher harmonic generation microscopy and deep learning
Authors: van Huizen Laura M. G. , Blokker Max , Rip Yael , Veta Mitko , Mooij Kalverda Kirsten A. , Bonta Peter I. , Duitman Jan Willem , Groot Marie Louise 
Published: June 2023

BackgroundIn diseases such as interstitial lung diseases (ILDs), patient diagnosis relies on diagnostic analysis of bronchoalveolar lavage fluid (BALF) and biopsies. Immunological BALF analysis includes differentiation of leukocytes by standard cytological techniques that are labor-intensive and time-consuming. Studies have shown promising leukocyte identification performance on blood fractions, using third harmonic generation (THG) and multiphoton excited autofluorescence (MPEF) microscopy.ObjectiveTo extend leukocyte differentiation to BALF samples using THG/MPEF microscopy, and to show the potential of a trained deep learning algorithm for automated leukocyte identification and quantification.MethodsLeukocytes from blood obtained from three healthy individuals and one asthma patient, and BALF samples from six ILD patients were isolated and imaged using label-free microscopy. The cytological characteristics of leukocytes, including neutrophils, eosinophils, lymphocytes, and macrophages, in terms of cellular and nuclear morphology, and THG and MPEF signal intensity, were determined. A deep learning model was trained on 2D images and used to estimate the leukocyte ratios at the image-level using the differential cell counts obtained using standard cytological techniques as reference.ResultsDifferent leukocyte populations were identified in BALF samples using label-free microscopy, showing distinctive cytological characteristics. Based on the THG/MPEF images, the deep learning network has learned to identify individual cells and was able to provide a reasonable estimate of the leukocyte percentage, reaching >90% accuracy on BALF samples in the hold-out testing set.ConclusionsLabel-free THG/MPEF microscopy in combination with deep learning is a promising technique for instant differentiation and quantification of leukocytes. Immediate feedback on leukocyte ratios has potential to speed-up the diagnostic process and to reduce costs, workload and inter-observer variations.
The relationship between family history of cancer and cancer attitudes & beliefs within the Community Initiative Towards Improving Equity and Health Status (CITIES) cohort
Authors: Lin Li , Zhang Xiaochen , Yu Mengda , Bernardo Brittany , Adeyanju Toyin , Paskett Electra D. 
Published: June 2023

Objective
To determine the relationship between family history of cancer with cancer attitudes and beliefs (CABs) and cancer screening knowledge.

Methods
This study used data collected for the Community Initiative Towards Improving Equity and Health Status (CITIES) project which surveyed Ohioans ages 21–74. In the current analysis, we included data on age, gender, race, marital status, education, income, financial security, health insurance, CABs, knowledge about the correct age to begin cancer screenings, and presence of a first-degree relative with cancer. Multivariable logistic regression was used to examine the association of family history of cancer with CABs and knowledge about the correct age to begin cancer screening.

Results
Participants were predominantly over the age of 41, female, and white. Out of 603 participants, 295 (48.92%) reported not having a first-degree relative with cancer and 308 (51.08%) reported having a first-degree relative with cancer. Overall, 109 (18.08%) participants reported negative CABs, 378 (62.69%) reported moderate CABs, and 116 (19.24%) reported positive CABs. Participants who reported a first-degree relative with cancer were more likely to report positive CABs, but the association was not significant (p = .11). We observed that older, more educated, and married participants were more likely to have positive CABs (all p < 0.05). Family history of cancer was not associated with differences in knowledge about the correct age for beginning colorectal cancer screening (p = .85) and mammography (p = .88).

Conclusions
Having a first-degree relative with cancer was not found to be associated with CABs or knowledge about cancer screening. However, age and socioeconomic status were associated with more positive CABs and increased knowledge about cancer screening. Future research should focus on standardizing a CABs scale and expanding the generalizability of our findings.
Effect of COVID-19 on dental service delivery in Fiji: Perspective of Dental Officers (Dos) and Dental Managers (DMs)
Authors: Kajal Kartika , Mohammadnezhad Masoud 
Published: June 2023

Background
Corona Virus Disease 2019 (COVID-19) was declared a global pandemic by the World Health Organization (WHO) has had significant impact on dentistry in Fiji. Due to lack of previous study, this study aims to explore the perspective of Dental Officers (DOs) and Dental Managers (DMs) on the effects of COVID-19 on dental service delivery in Fiji Islands.

Methods
This qualitative study was conducted amongst 30 DOs and 17 DMs between 9th August to 12th September, 2021. It was conducted in the government dental clinics, private dental clinics and the School of Dentistry and Oral Health clinic (SDOH), in the Central Division, Fiji. The study settings were randomly selected. Purposive sampling method was used for the selection of participants who met the study criteria. Semi-structure open ended questionnaires were used for data collection through in-depth interviews via zoom. Manual thematic analysis of the data was conducted to derive themes and codes.

Results
The participants interviewed for the study included more female DOs (66.7%) and male DMs (58.8%). Seven themes emerged from data analysis: range of services delivered, appointment versus walk-in patients for aerosol generating procedures (AGPs), impact of pandemic on clinic opening hours, impact of COVID-19 on patient numbers, quality of services delivered, resources and infrastructure, perceptions about the burden of disease.

Conclusion
COVID-19 has significantly affected dental service delivery. Mostly emergency dental services were delivered. AGPs were delivered on appointment basis. Most participants stated the quality of services had improved. Participants stated that they were not given adequate resources and the infrastructure was not up to standard to provide dental services during the pandemic. The dental disease burden had increased during the pandemic as per the participants. Future research can be conducted amongst other dental professionals in other divisions of the country.
Cultural Appropriation in Fashion and Entertainment, Yuniya Kawamura and Jung-Whan Marc de Jong (2021)
Authors: Williams Gwyneth I. 
Published: June 2023

Review of: Cultural Appropriation in Fashion and Entertainment, Yuniya Kawamura and Jung-Whan Marc de Jong (2021)
London and New York: Bloomsbury Visual Arts, 218 pp.,
ISBN 978-1-35017-055-1, p/bk, $20.96
ISBN 978-1-3501-7054-4, h/bk, $63.00
ISBN 978-1-3501-7058-2, ePUB, $16.17
ISBN 978-1-3501-7056-8, e-book, $16.17
circNFATC3 facilitated the progression of oral squamous cell carcinoma via the miR-520h/LDHA axis
Authors: Xie Hongguo , Lu Xiaopeng 
Published: June 2023

Abstract
The aim of this study was to verify the effects of circular RNA nuclear factor of activated T-cells, cytoplasmic 3 (circNFATC3), in oral squamous cell carcinoma (OSCC) development. The levels of circNFATC3, microRNA-520h (miR-520h), and lactate dehydrogenase A (LDHA) were measured by qRT-PCR and western blot analysis. The cellular functions were assessed by using commercial kits, MTT assay, EdU assay, flow cytometry analysis, and transwell assay. The interactions between miR-520h and circNFATC3 or LDHA were confirmed by dual-luciferase reporter assay. Finally, the mice test was enforced to evaluate the character of circNFATC3. We observed that the contents of circNFATC3 and LDHA were upregulated and miR-520h levels were downregulated in OSCC tissues compared with those in paracancerous tissues. For functional analysis, circNFATC3 knockdown repressed the cell glycolysis metabolism, cell proliferation, migration, and invasion, although it improved cell apoptosis in OSCC cells. LDHA could regulate the development of OSCC. circNFATC3 acted as a miR-520h sponge to modulate LDHA expression. In addition, the absence of circNFATC3 subdued tumor growth in vivo. In conclusion, circNFATC3 promoted the advancement of OSCC by adjusting the miR-520h/LDHA axis.
Prone position effect in intensive care patients with SARS-COV-2 pneumonia
Authors: Rebelo Oliveira Sandra Manuela , Ferreira Alexandra Marisa da Silva , Silva Paulo Jorge Ventura , Pinto Cristina Susana Sousa , Campello Maria Glória Cabral , Carvalho Amâncio António de Sousa 
Published: June 2023

AbstractVentilation in the prone position (PP) has been used for decades in patients with acute respiratory distress syndrome (ARDS) and is associated with a reduction in mortality rate. Its application has been extended to patients with SARS-Cov-2 pneumonia and is recommended by the main international organizations. The objective is to evaluate the effects of PP on the outcomes of patients with SARS-Cov-2 pneumonia admitted to a multipurpose intensive care unit. This is a quantitative, quasi-experimental, single-group, longitudinal and retrospective study. Data were collected based on clinical records. Data were processed using SPSS (version 26.0). PP significantly increased oxygenation in patients with SARS-Cov-2 pneumonia, with a mean increase of 21.27% between the PaO2/FiO2ratio before and after the PP. However, its effectiveness was inversely proportional to the number of cycles performed and the timing of orotracheal intubation. PP improves oxygenation in patients with SARS-Cov-2 pneumonia. However, multiple PP sessions are not beneficial, as this procedure is no longer effective after the fourth cycle. This study thus contributes to better management in the approach of critically ill patients with SARS-Cov-2 pneumonia.