bluuebunny/crossref_metadata_2025_split huggingface dataset:
Genome‐wide analysis facilitates estimation of the amount of male contribution in meiotic gynogenetic three‐spined stickleback (Gasterosteus aculeatus)
Authors: Currey Mark C. , Walker Charline , Bassham Susan , Healey Hope M. , Beck Emily A. , Cresko William A. 
Published: January 2023

AbstractGynogenetic embryos – those inheriting only maternal DNA – can be experimentally created by fertilizing eggs with radiation‐treated sperm containing inactivated paternal chromosomes. Diploidy in the zygotes can be maintained through prevention of the second meiosis or restored by preventing the first mitosis after the maternal chromosome complement has been replicated. These gynogenetic organisms are useful in many fields including aquaculture, evolutionary biology and genomics. Although gynogenetic organisms have been created in numerous species, the completeness of uni‐parental inheritance has often been assumed rather than thoroughly quantified across the genome. Instead, when tests of uni‐parental inheritance occur, they typically rely on well‐studied genetically determined phenotypes that represent a very small sub‐set of the genome. Only assessing small genomic regions for paternal inheritance leaves the question of whether some paternal contributions to offspring might still have occurred. In this study, the authors quantify the efficacy of creating gynogenetic diploid three‐spined stickleback fish (Gasterosteus aculeatus). To this end, the authors mirrored previous assessments of paternal contribution using well‐studied genetically determined phenotypes including sex and genetically dominant morphological traits but expanded on previous studies using dense restriction site‐associated DNA sequencing (RAD‐seq) markers in parents and offspring to assess paternal inheritance genome‐wide. In the gynogenetic diploids, the authors found no male genotypes underlying their phenotypes of interest – sex and dominant phenotypic traits. Using genome‐wide assessments of paternal contribution, nevertheless, the authors found evidence of a small, yet potentially important, amount of paternally “leaked” genetic material. The application of this genome‐wide approach identifies the need for more widespread assessment of paternal contributions to gynogenetic animals and promises benefits for many aspects of aquaculture, evolutionary biology and genomics.
A simulated marine heatwave impacts European sea bass sperm quantity, but not quality
Authors: Geffroy Benjamin , Sandoval‐Vargas Leydy , Boyer‐Clavel Myriam , Pérez‐Atehortúa Maritza , Lallement Stephane , Isler Iván Valdebenito 
Published: January 2023

AbstractRapid environmental changes will be the major challenge that most biota will have to deal with in the near future. Extreme events, such as marine heatwaves, are becoming more frequent and could be spatially uniform at a regional scale for a relatively long period of time. To date, most research studies on heatwaves have focused on sessile organisms, but these extreme events can also impact mobile species. Here, a 3‐week marine heatwave was simulated to investigate its effects on the male reproductive performance of a Mediterranean Sea emblematic species, the European sea bass Dicentrarchus labrax. Males from the control condition (c. 13°C) produced significantly more sperm than those exposed to a relatively warm thermal treatment (c. 16°C). Nonetheless, neither the percentage of motile spermatozoa nor most of the other sperm motility parameters were significantly affected by the rearing temperature over the whole period. Overall, the results of this study suggest only moderated effects of a potential winter heatwave on the reproductive performance of male European sea bass.
Understanding the regulation of cereal grain filling: The way forward
Authors: Ma Bin , Zhang Lin , He Zuhua 
Published: January 2023

AbstractDuring grain filling, starch and other nutrients accumulate in the endosperm; this directly determines grain yield and grain quality in crops such as rice (Oryza sativa), maize (Zea mays), and wheat (Triticum aestivum). Grain filling is a complex trait affected by both intrinsic and environmental factors, making it difficult to explore the underlying genetics, molecular regulation, and the application of these genes for breeding. With the development of powerful genetic and molecular techniques, much has been learned about the genes and molecular networks related to grain filling over the past decades. In this review, we highlight the key factors affecting grain filling, including both biological and abiotic factors. We then summarize the key genes controlling grain filling and their roles in this event, including regulators of sugar translocation and starch biosynthesis, phytohormone‐related regulators, and other factors. Finally, we discuss how the current knowledge of valuable grain filling genes could be integrated with strategies for breeding cereal varieties with improved grain yield and quality.
COUP‐TFII plays a role in cAMP‐induced Schwann cell differentiation and in vitro myelination by up‐regulating Krox20
Authors: Han Sang‐Heum , Kim Young Hee , Park Su‐Jeong , Cho Jun‐Gi , Shin Yoon Kyung , Hong Young Bin , Yun Jeanho , Han Jin‐Yeong , Park Hwan Tae , Park Joo‐In 
Published: January 2023

AbstractSchwann cells (SCs) are known to produce myelin for saltatory nerve conduction in the peripheral nervous system (PNS). Schwann cell differentiation and myelination processes are controlled by several transcription factors including Sox10, Oct6/Pou3f1, and Krox20/Egr2. Chicken ovalbumin upstream promoter‐transcription factor II (COUP‐TFII/NR2F2) is an orphan receptor that plays a role in the development and differentiation. However, the role of COUP‐TFII in the transcriptional regulatory network of SC differentiation has not been fully identified yet. Thus, the objective of this study was to investigate the role and molecular hierarchy of COUP‐TFII during cAMP‐induced SC differentiation. Our results showed that dibutyryl‐cAMP (db‐cAMP) increased expression levels of COUP‐TFII along with the expressions of Oct6, Krox20, and myelin‐related genes known to be related to SC differentiation. Our mechanistic studies showed that COUP‐TFII acted downstream of Hsp90/ErbB2/Gab1/ERK‐AKT pathway during db‐cAMP‐induced SC differentiation. In addition, we found that COUP‐TFII induced Krox20 expression by directly binding to Krox20‐MSE8 as revealed by chromatin immunoprecipitation assay and promoter activity assay. In line with this, the expression of COUP‐TFII was increased before up‐regulation of Oct6, Krox20, and myelin‐related genes in the sciatic nerves during early postnatal myelination period. Finally, COUP‐TFII knockdown by COUP‐TFII siRNA or via AAV‐COUP‐TFII shRNA in SCs inhibited db‐cAMP‐induced SC differentiation and in vitro myelination of sensory axons, respectively. Taken together, these findings indicate that COUP‐TFII might be involved in postnatal myelination through induction of Krox20 in SCs. Our results present a new insight into the transcriptional regulatory mechanism in SC differentiation and myelination.image
Diagnostic accuracy of a portable device (Bruxoff®) to measure sleep bruxism
Authors: Yanez‐Regonesi Fernanda , Eisa Elfatih , Judge Stephanie , Carlson Charles , Okeson Jeffrey , Moreno‐Hay Isabel 
Published: January 2023

AbstractBackgroundDiagnosis of sleep bruxism (SB) challenges clinicians every day due to multiple forms of assessment tools available, including self‐reported questionnaires, clinical examinations, portable devices and laboratory polysomnography (PSG). PSG has become the gold standard for evaluating SB, but it can be limited due to cost and restricted accessibility which often is characterised by long waiting times. Hence, there is a need for the development of a reliable method that can assess SB in a simple and portable manner, which would offer acceptable sensitivity and specificity to evaluate SB.ObjectiveThe objective of this study was to investigate reliability and validity of the Bruxoff® device for the diagnosis of SB compared to the PSG.MethodsForty‐nine subjects underwent one night of polysomnographic study with simultaneous recording with the Bruxoff® device. Rhythmic masticatory muscle activity (RMMA) index was scored according to published criteria. Pearson correlation, Bland–Altman plot and receiver operating characteristic (ROC) curve outcomes were used to quantify the agreement between both methods.ResultsReceiver operating characteristic analysis showed an acceptable accuracy for the Bruxoff® with sensitivity of 83.3% and specificity of 72% when the cut‐off was set at two events per hour. Pearson correlation analysis showed a nearly significant correlation between PSG and Bruxoff® for RMMA index (r = .282 p = .071) and for total SB episodes per night (r = .295 p = .058). Additionally, the Bland–Altman plot revealed a consistent and systematic difference in the measurement of events between devices.ConclusionThe Bruxoff® device appears to be a promising diagnostic method for clinical use, but further study is needed.
Midadolescents’ Language Learning at School: Toward More Just and Scientifically Rigorous Practices in Research and Education
Authors: Uccelli Paola 
Published: January 2023

AbstractWhich theoretical and empirical insights can inform language‐in‐education research that advances equitable and high‐quality learning at school? In this three‐part article, I first draw from various sources to foreground the urgent need to counteract linguicism and epistemic injustices and to commit to more just and rigorous scientific practices in research and education. Based on findings from my own research, I then argue that more just and rigorous practices need to attend to midadolescents’ individual differences in language learning. In response, I present insights from recent innovative evidence‐based pedagogical frameworks and tools that reveal promising paths forward. I close by calling for research with a dual focus on equity and rigor that supports moving away from “pedagogies of silence,” which silence marginalized students’ voices, damaging their identities and obstructing their learning, towards “pedagogies of voices,” which affirm all learners’ voices while amplifying their language repertoires and their critical learning.
Does metabolite matter? Defining target itraconazole and hydroxy‐itraconazole serum concentrations for blastomycosis
Authors: Firkus Danielle , Abu Saleh Omar M. , Enzler Mark J. , Jannetto Paul J. , Mara Kristin , Vergidis Paschalis , Rivera Christina G. , Stevens Ryan W. 
Published: January 2023

AbstractBackgroundItraconazole is the recommended first‐line treatment for mild‐to‐moderate blastomycosis and consolidation treatment of moderate‐to‐severe disease. Itraconazole is metabolised into three metabolites, including an active metabolite hydroxy‐itraconazole. Literature provides little evidence indicating whether therapeutic drug monitoring targets should be based on itraconazole parent compound alone or a sum of itraconazole and hydroxy‐itraconazole serum concentrations.ObjectivesThis study aims to compare clinical outcomes and adverse drug events (ADEs) of combined itraconazole and hydroxy‐itraconazole concentrations versus itraconazole parent compound alone in patients with blastomycosis.Patients/MethodsThis study was a retrospective cohort review of patients ≥18 years with probable or proven Blastomyces infection who received itraconazole with at least one documented serum itraconazole concentration. The primary outcome was rate of partial or complete treatment response across three patient groups: (1) Itraconazole parent compound >1.0 mcg/ml (parent), (2) parent compound <1.0 mcg/ml, but a combined itraconazole and hydroxy‐itraconazole >1.0 mcg/ml (combined) and (3) failure to achieve a combined or parent concentration >1.0 mcg/ml (subtherapeutic) for >75% of the duration of itraconazole therapy.ResultsA total of 80 patients were included (parent = 32, combined = 36, subtherapeutic = 12). No statistically significant difference was observed for rate of partial or complete treatment response (97% parent vs 94% combined, p = .99). Significantly higher mortality due to blastomycosis was observed in patients in the subtherapeutic group (0% parent vs 3% combined vs 25% subtherapeutic, p = .01).ConclusionsThis study supports an itraconazole therapeutic target combining itraconazole and hydroxy‐itraconazole >1.0 mcg/ml for blastomycosis treatment.
Nutrition knowledge and influence on diet in the carer–client relationship in residential care settings for people with intellectual disabilities
Authors: Özdemir Aslıhan , Hall Rebecca , Lovell Andrew , Ellahi Basma 
Published: January 2023

AbstractPeople with intellectual disabilities generally have poorer health outcomes compared with those who do not, including outcomes related to nutrition‐related non‐communicable diseases. Carers support people with intellectual disabilities in many aspects including habitual shopping and preparation of food, but their own nutrition knowledge and the influence this may have on dietary intakes of clients is unknown. We explored the nutrition knowledge of carers of people with intellectual disabilities in residential care settings, their dietary habits and their influence on clients' food shopping and preparation and therefore the diet consumed by their clients. Ninety‐seven carers belonging to a large independent care sector organisation specialising in the care of people with an intellectual disability completed a validated general nutrition knowledge and behaviour questionnaire. Seventeen carers from the residential care settings were interviewed to contextualise practice. Knowledge about key dietary recommendations scored highly. Carers who had more work experience were found to have higher scores in ‘making everyday food choices’ (p = 0.034). Daily consumption of fruit and vegetables (at least one portion per day) was observed (for fruit by 46% of the carers and for vegetables by 60% of the carers), whilst most carers reported avoiding consuming full‐fat dairy products, sugary foods and fried foods. The concept of a healthy diet; typical dietary habits of clients; role in food acquisition; and training in nutrition emerged as themes from the interviews. Carers discussed various topics including the importance of a balanced diet, cooking fresh foods and control of food portion sizes for clients relative to the care philosophy of a client‐centred approach, which encapsulates client autonomy. Gaps in knowledge around specific nutrients, making healthy choices and cooking skills remain. Carers have an influence on clients' dietary choices; they are able to provide healthy meals and share good dietary habits with clients. Further training in nutrition is recommended for impact on clients' health.
Anatomical and Biomechanical Stability of Single/Double Screw‐Cancellous Bone Fixations of Regan–Morry Type III Ulnar Coronoid Fractures in Adults: CT Measurement and Finite Element Analysis
Authors: Ye Hao , Yang Yongchao , Xing Tingyang , Tan Guirong , Jin Shuxun , Zhao Zhichao , Zhang Weikang , Li Yanyan , Zhang Lei , Wang Jianshun , Zheng Rongmei , Lu Yun , Wu Lijun 
Published: January 2023

ObjectiveAt present, it is still uncertain whether single screw has the same stability as double screws in the treatment of ulnar coronal process basal fracture (Regan–Morry type III). So, we aimed to compare the pull‐out force and anti‐rotation torque of anterior single/double screw‐cancellous bone fixation (aSSBF, aDSBF) in this fracture, and further study the influencing factors on anatomical and biomechanical stability of smart screw internal fixations.MethodsA total of 63 adult volunteers with no history of elbow injury underwent elbow CT scanning with associated three‐dimensional reconstruction that enabled the measurements of bone density and fixed length of the proximal ulna and coronoid. The models of coronal process basal fracture, aSSBF and aDSBF, were developed and validated. Using the finite element model test, the sensitivity analysis of pull‐out force and rotational torque was carried out.ResultsThe pull‐out force of aSSBF model was positively correlated with the density of the cancellous bone and linearly related to the fixed depth of the screw. The load pattern of pull‐out force of aDSBF model was similar to that of aSSBF model. The ultimate torque of aDSBF model was higher than that of aSSBF model, but the load pattern of ultimate torque of both models was similar to each other when the fracture reset was satisfactory, and the screw nut attaches closely to coronoid process. Moreover, with enhancement of initial pre‐tightening force, the increase of ultimate torque of both models was small.ConclusionsIn addition to three pull‐out stability factors of smart screw fixations, fracture surface fitting degree and nut fitting degree are the other two important anatomical and biomechanical stability factors of smart screw fixations both for rotational stability. When all pull‐out stability and rotational stability factors meet reasonable conditions simultaneously, single or double screw fixation methods are stable for the treatments of ulnar coronoid basal fractures.
Chinese regions' participation in global value chains and the associated global transmission of export price and quantity shocks
Authors: Egger Peter H. , Li Jie , Zhao Yu 
Published: January 2023

AbstractThis paper proposes an imputation of a global input‐output matrix, where China is broken up into 332 prefecture‐type regions in three years of data, 2007, 2012, and 2017. Using the resulting global input‐output matrix, the paper documents that sizable spillover effects exist with regard to economic volatility. In particular, such volatility spillovers are important for prices and somewhat less so for quantity shocks. We demonstrate that individual Chinese prefectures are large recipients and donors of such shocks. All Chinese prefectures together have a very large impact on the world economy in terms of the considered volatility shocks.
Mucormycosis causing oral and nasal osteonecrosis in a case of acute myeloid leukemia
Authors: Lisboa Mariah Luz , Grando Liliane Janete , Kretzer Sara Letícia , Tomazelli Karin Berria , Bianco Bianca Carla , Santos da Silva Maria Claudia , Kosmann Cleumara , Meurer Maria Inês 
Published: January 2023

AbstractAimsMucormycosis is a rare and aggressive fungal infection with a high mortality rate because of its rapidly progressive and destructive nature. The oral cavity is often affected under opportunistic conditions. We report a 34‐year‐old woman diagnosed with acute myeloid leukemia complained of slight swelling on the right side of her face with toothache and gingival swelling. An incisional biopsy was performed, and the specimen analysis revealed broad aseptate hyphae with a ribbon‐like appearance, which is characteristic of opportunistic Mucorales infection.Methods and ResultsThe oral lesion worsened, and invasion of the fungal infection into the maxillary sinus, nasal cavity, ethmoidal air cells, and sphenoid and frontal sinuses was observed. Partial maxillectomy was performed concomitantly with the ongoing chemotherapy for leukemia. A maxillofacial prosthesis was used for functional rehabilitation.ConclusionSuccessful management requires a multimodal approach. In this case, the patient required different systemic approaches for treating leukemia and the fungal infection as well as rehabilitation with an obturator prosthesis.
Virological failure and adherence to antiretroviral therapy in adolescents and young adults living with human immunodeficiency virus
Authors: Daltro Adeilton Cleison Borges , Almeida Carla Santos , Unfried Adriana Graça Costa , de Aquino Talita Rocha , Travassos Ana Gabriela Álvares 
Published: January 2023

AbstractObjectiveHIV (human immunodeficiency virus) infection remains a major public health challenge. Infected young people at any age are less likely to adhere to care in a timely manner and to maintain a suppressed VL. This review aims to identify factors associated with virologic failure and adherence to drug therapy in adolescents and young adults (10–24 years) living with HIV.MethodsSystematic review using the PubMed and Virtual Health Library databases and including articles published between 2009 and 2021. Data were analysed in six categories: individual factors, pharmacological/therapy‐related aspects, factors related to HIV/acquired immunodeficiency syndrome (AIDS) infection, HIV/AIDS stigma, social support and health system/services. The study's protocol was registered on the PROSPERO platform (CRD42020167581).ResultsA total of 19,819 articles were found in the initial search and 31 studies were included in this systematic review. Most studies were carried out on the African continent. Male sex, alcohol use, low education, adverse effects of medication, lack of social support, stigma related to HIV/AIDS, need for transportation to access the health service and forgetfulness were linked to poor adherence to therapy. Good adherence was achieved with sufficient nutrition, good social support, greater confidence in the use of therapy and fewer ART side effects. Low levels of CD4, alcohol use, substance abuse, low education, non‐adherence to medication and forgetfulness were linked to virological failure.ConclusionIndividual, social and structural factors constitute barriers to adherence to ART among adolescent and young adults. It is necessary to know the difficulties related to the use of therapy to work out specific strategies that create conditions to improve medication adherence and viral suppression, reducing the levels of virological failure in this population.
The role of mitochondria in the peri‐implant microenvironment
Authors: Zhang Xidan , Sun Jiyu , Zhou Min , Li Chen , Zhu Zhuoli , Gan Xueqi 
Published: January 2023

AbstractOsseointegration is a dynamic biological process in the local microenvironment adjacent to a bone implant, which is crucial for implant performance and success of the implant surgery. Recently, the role of mitochondria in the peri‐implant microenvironment during osseointegration has gained much attention. Mitochondrial regulation has been verified to be essential for cellular events in osseointegration and as a therapeutic target for peri‐implant diseases in the peri‐implant microenvironment. In this review, we summarize our current knowledge of the key role of mitochondria in the peri‐implant milieu, including the regulation of mitochondrial reactive oxygen species and mitochondrial metabolism in angiogenesis, the polarization of macrophage immune responses, and bone formation and resorption during osseointegration, which will contribute to the research field and the development of new treatment strategies to improve implant success. In addition, we indicate limitations in our current understanding of the regulation of mitochondria in osseointegration and suggest topics for further study.