bluuebunny/crossref_metadata_2025_split huggingface dataset:
Cystathionine β-synthase affects organization of cytoskeleton and modulates carcinogenesis in colorectal carcinoma cells
Authors: Liskova Veronika , Chovancova Barbora , Babula Petr , Rezuchova Ingeborg , Pavlov Kristina Ploth , Matuskova Miroslava , Krizanova Olga 
Published: July 2023

BackgroundCystathionine β-synthase (CBS), one of three enzymes that endogenously produce hydrogen sulfide, is extensively studied for its relevance in the cells of various tumors. In our previous work, we observed that the immunofluorescence pattern of CBS is very similar to that of tubulin and actin. Therefore, we focused on the potential interaction of CBS with cytoskeletal proteins β-actin and β-tubulin and the functional relevance of the potential interaction of these proteins in colorectal carcinoma cell lines.MethodsTo study the potential interaction of CBS with cytoskeletal proteins and its functional consequences, a CBS-knockout DLD1 (DLDx) cell line was established by using the CRISPR/Cas9 gene editing method. The interaction of the selected cytoskeletal protein with CBS was studied by immunoprecipitation, Western blot analysis, immunofluorescence, and proximity ligation assay. The functional consequences were studied by proliferation and migration assays and by generation of xenografts in SCID/bg mice.ResultsWe have found that CBS, an enzyme that endogenously produces H2S, binds to cytoskeletal β-tubulin and, to a lesser extent, also to β-actin in colorectal carcinoma-derived cells. When CBS was knocked out by the CRISPR/Cas9 technique (DLDx), we observed a de-arranged cytoskeleton compared to the unmodified DLD1 cell line. Treatment of these cells with a slow sulfide donor GYY4137 resulted in normal organization of the cytoskeleton, thus pointing to the role of CBS in microtubule dynamics. To evaluate the physiological importance of this observation, both DLD1 and DLDx cells were injected into SCID/bg mice, and the size and mass of the developed xenografts were evaluated. Significantly larger tumors developed from DLDx compared to the DLD1 cells, which correlated with the increased proliferation of these cells.ConclusionsTaken together, in colorectal cancer DLD1 cells, CBS binds to the cytoskeleton, modulates microtubule dynamics, and thus affects the proliferation and migration in the colorectal carcinoma stable cell line.
Genomic versus phenotypic selection to improve corn borer resistance and grain yield in maize
Authors: Gesteiro Noemi , Ordás Bernardo , Butrón Ana , de la Fuente María , Jiménez-Galindo José Cruz , Samayoa Luis Fernando , Cao Ana , Malvar Rosa Ana 
Published: July 2023

IntroductionThe study of yield and resistance/tolerance to pest are related traits fundamental for maize breeding programs. Genomic selection (GS), which uses all marker information to calculate genomic breeding values, is presented as an emerging alternative to phenotypic and marker-assisted selections for improving complex traits controlled by many genes with small effects. Therefore, although phenotypic selection (PS) has been effective for increasing resistance and yield under high infestation with maize stem borers, higher genetic gains are expected to be obtained through GS based on the complex architecture of both traits. Our objective was to test whether GS is more effective than PS for improving resistance and/or tolerance to maize stem borers and grain yield.MethodsFor this, we compared different selection programs based on phenotype and genotypic value for a single trait, resistance or yield, and for both traits together.Results and discussionWe obtained that GS achieved the highest genetic gain for yield, meanwhile phenotypic selection for yield was the program that achieved the highest reduction of tunnel length, but was ineffective for increasing yield. However, phenotypic or genomic selection for increased resistance may be more effective in improving both traits together; although the gains per cycle would be small for both traits.
Virtual interdisciplinary collaboration during the COVID-19 pandemic: pain and joy in an international joint university
Authors: Lu Jinjin 
Published: July 2023

BackgroundThe COVID-19 pandemic has brought interdisciplinary academics and research students many uncertainties and challenges in adapting to new communication styles. Compared with other academics in the same field, interdisciplinary academics might face more challenges in transitioning from traditional face-to-face communication to virtual communication.ObjectiveThis study aimed to explore the pain and joy of using Western and Chinese localized communication channels in experienced interdisciplinary academics (N = 10) and young research students (N = 14) during the pandemic. Among them, 14 are Europeans and 10 are Chinese.MethodMeeting records and participants' reflective writing were used as qualitative data.ResultsWe identified five key themes: two were tied to personal and behavioral issues, two were involved in management issues, and one dealt with topic choice issues.ConclusionConsidering that virtual interdisciplinary teamwork is likely to continue in the post-pandemic period, it is necessary to implement measures such as technical training and voluntary assistants to help alleviate some of the issues that make virtual meetings difficult for participants. Study limitations and future directions are also discussed.
Chatbot-assisted therapy for patients with methamphetamine use disorder: a preliminary randomized controlled trial
Authors: Chun-Hung Lee , Guan-Hsiung Liaw , Wu-Chuan Yang , Yu-Hsin Liu 
Published: July 2023

BackgroundMethamphetamine (MA) use disorder is associated with a large public health burden. Despite the therapeutic effects of psychosocial interventions based on current evidence, finding an approach to retain patients in treatment remains a real-world challenge. The rapid development of mobile health (mHealth) systems suggests the potential to provide real-time personalized care at any time and from any location, minimize barriers to treatment, maximize use, and promote the dissemination of accessible therapeutic tools in at-risk populations. Our study aimed to investigate the feasibility and effectiveness of chatbots for the treatment of MA use disorder.MethodThe inclusion criteria were (a) a diagnosis of MA use disorder as defined by the DSM-5, (b) age between 18 and 65 years, (c) no acute exacerbation of severe mental illness during the initial assessment, such as schizophrenia or bipolar I disorder, (d) willingness to participate in standard outpatient treatment for ≥ 6 months, and (e) an Android phone. Participants were randomly allocated to either a chatbot-assisted therapy via smartphone (CAT) group or a control group following simple randomization procedures (computerized random numbers) without blinding. All participants were followed up for 6 months. Treatment retention and monthly urine test results were analyzed as outcome measures. Participants' satisfaction with CAT was also assessed.ResultsIn total, 50 and 49 participants were allocated to the CAT and control groups, respectively. There were no significant differences in retention time between the two treatment groups (df = 1, p = 0.099). The CAT group had fewer MA-positive urine samples than the control group (19.5% vs. 29.6%, F = 9.116, p = 0.003). The proportion of MA-positive urine samples was positively correlated with the frequency of MA use (r = 0.323, p = 0.001), severity of MA use disorder (r = 0.364, p &lt; 0.001), and polysubstance use (r = 0.212, p = 0.035), and negatively correlated with readiness to change (r = −0.330, p = 0.001). Totally 55 participants completed the study at the 6-month follow-up and 60% reported relative satisfaction.ConclusionParticipants in this study had favorable acceptance and generally positive outcomes, which indicates that chatbot is feasible for treating people who use MA.
The heterogeneity of attenuated and brief limited psychotic symptoms: association of contents with age, sex, country, religion, comorbidities, and functioning
Authors: Theisen Christian , Rosen Marlene , Meisenzahl Eva , Koutsouleris Nikolaos , Lichtenstein Theresa , Ruhrmann Stephan , Kambeitz Joseph , Kambeitz-Ilankovic Lana , Riecher-Rössler Anita , Chisholm Katharine , Upthegrove Rachel , Antonucci Linda A. , Bertolino Alessandro , Pigoni Alessandro , Salokangas Raimo K. R. , Pantelis Christos , Wood Stephen J. , Lencer Rebekka , Falkai Peter , Hietala Jarmo , Brambilla Paolo , Schmidt André , Andreou Christina , Borgwardt Stefan , Osman Naweed , Schultze-Lutter Frauke 
Published: July 2023

IntroductionThe Attenuated Psychosis Symptoms (APS) syndrome mostly represents the ultra-high-risk state of psychosis but, as does the Brief Intermittent Psychotic Symptoms (BIPS) syndrome, shows a large variance in conversion rates. This may be due to the heterogeneity of APS/BIPS that may be related to the effects of culture, sex, age, and other psychiatric morbidities. Thus, we investigated the different thematic contents of APS and their association with sex, age, country, religion, comorbidity, and functioning to gain a better understanding of the psychosis-risk syndrome.MethodA sample of 232 clinical high-risk subjects according to the ultra-high risk and basic symptom criteria was recruited as part of a European study conducted in Germany, Italy, Switzerland, and Finland. Case vignettes, originally used for supervision of inclusion criteria, were investigated for APS/BIPS contents, which were compared for sex, age, country, religion, functioning, and comorbidities using chi-squared tests and regression analyses.ResultWe extracted 109 different contents, mainly of APS (96.8%): 63 delusional, 29 hallucinatory, and 17 speech-disorganized contents. Only 20 contents (18.3%) were present in at least 5% of the sample, with paranoid and referential ideas being the most frequent. Thirty-one (28.5%) contents, in particular, bizarre ideas and perceptual abnormalities, demonstrated an association with age, country, comorbidity, or functioning, with regression models of country and obsessive-compulsive disorders explaining most of the variance: 55.8 and 38.3%, respectively. Contents did not differ between religious groups.ConclusionPsychosis-risk patients report a wide range of different contents of APS/BIPS, underlining the psychopathological heterogeneity of this group but also revealing a potential core set of contents. Compared to earlier reports on North-American samples, our maximum prevalence rates of contents were considerably lower; this likely being related to a stricter rating of APS/BIPS and cultural influences, in particular, higher schizotypy reported in North-America. The various associations of some APS/BIPS contents with country, age, comorbidities, and functioning might moderate their clinical severity and, consequently, the related risk for psychosis and/or persistent functional disability.
Development and validation of a nomogram to predict suicidal behavior in female patients with mood disorder
Authors: Liang Sixiang , Liu Xinyu , Li Dan , Zhang Jinhe , Zhao Guangwei , Yu Hongye , Zhao Xixi , Sha Sha 
Published: July 2023

IntroductionThis study aims to explore the risk factors associated with suicidal behavior and establish predictive models in female patients with mood disorders, specifically using a nomogram of the least absolute shrinkage and selection operator (LASSO) regression.MethodsA cross-sectional survey was conducted among 396 female individuals diagnosed with mood disorders (F30-F39) according to the International Classification of Diseases and Related Health Problems 10th Revision (ICD-10). The study utilized the Chi-Squared Test,t-test, and the Wilcoxon Rank-Sum Test to assess differences in demographic information and clinical characteristics between the two groups. Logistic LASSO Regression Analyses were utilized to identify the risk factors associated with suicidal behavior. A nomogram was constructed to develop a prediction model. The accuracy of the prediction model was evaluated using a Receiver Operating Characteristic (ROC) curve.ResultThe LASSO regression analysis showed that psychotic symptoms at first-episode (β= 0.27), social dysfunction (β= 1.82), and somatic disease (β= 1.03) increased the risk of suicidal behavior. Conversely, BMI (β= −0.03), age of onset (β= −0.02), polarity at onset (β= −1.21), and number of hospitalizations (β= −0.18) decreased the risk of suicidal behavior. The area under ROC curve (AUC) of the nomogram predicting SB was 0.778 (95%CI: 0.730–0.827,p&lt; 0.001).ConclusionThe nomogram based on demographic and clinical characteristics can predict suicidal behavior risk in Chinese female patients with mood disorders.
The relationship between depression, healthy lifestyle behaviors and internet addiction: a cross-sectional study of the athlete university students in Turkey
Authors: Çelik Demet Öztürk , Haney Meryem Öztürk 
Published: July 2023

IntroductionMental health research exists for university students in the areas of prevalence and association of depression, internet addiction and healthy lifestyle behaviors. However, the studies examining prevalence rates and these relationships in athlete university students are needed. This study aimed to evaluate the relationship between depression, healthy lifestyle behaviors and internet addiction among athlete university students in Turkey.MethodsA cross-sectional design was conducted, measuring depression using the Center for Epidemiologic Studies Depression Scale, healthy lifestyle behaviors (HPLP-II), and internet addiction (YIAT-SF). Predictive factors associated with depressive symptoms were estimated using binary logistic regression.ResultsOf the 501 participants, 61.3% were male, with a mean age of 21.45 years (SD: 3.19). Student-athletes were at risk of depressive symptoms (76.4%), internet addiction (34.4%) and had moderate healthy lifestyle behaviors (130.41 ± 22.93). No significant differences were found for age, smoking, time spent on social network, number of sports days, number of social networks use for depressive symptoms (p &gt; 0.05), however significant differences were found for depressive symptoms by sex and living with family status (p &lt; 0.05). Pathological internet addiction (OR: 12.74, 95% CI: 2.91–55.77) and low spiritual growth (OR: 0.854, 95% CI: 0.79–0.92) were found to be predictors of depressive symptoms within the athlete university students (p &lt; 0.001).ConclusionThe athlete university students are at increased risk for depressive symptoms and internet addiction and decreased healthy lifestyle behaviors. It is essential to establish programs to improve the mental health of students in universities and to strengthen the psychological counseling services offered to students. These findings can assist universities in establishing effective measures to improve mental health outcomes.
Toward an efficient determination of tissue-free water tritium in food
Authors: Ren Hong , Ma Xiaoxiang , Zhou Lei , Wang Peng , Chen Ting , Zou Xiao , Zou Hua , Yu Shunfei , Cao Yi , Lai Zhongjun , Lou Xiaoming , Cao Yiyao 
Published: July 2023

IntroductionTritium in the environment constitutes a radiological concern because it can become part of the hydrogen pool in environmental and biological reservoirs and thereby expose people to radiation.MethodsTissue-free water tritium (TFWT) analysis in food is an important subject of environmental radiation monitoring which plays an important role in the estimation of health risks from environmental tritium exposure. At present, tritium content in food is generally determined by liquid scintillation counter (LSC). To improve the analytical efficiency in tritium determination, we developed a novel method to treat TFWT in food using microporous membranes.ResultsThe microporous membrane treatment method developed in this study has the following characteristics: It has a wide range of application and can process TFWT samples with conductivity below 5 μS/cm. Sample loss for the microporous membrane treatment is approximately 5%. The average treatment time is only 5 min, significantly shortened compared with the currently used atmospheric distillation treatment method (1.5 h). The results of the comparison and spike experiment show that the samples prepared by microporous membrane treatment provides equally satisfactory tritium measurement results as classic distillation method.DiscussionThe developed microporous membrane method is simple to operate, efficient, and environmentally friendly, and effectively improves the analysis efficiency of TFWT in food.
Association between opioid prescription profiles and adverse health outcomes in opioid users referred for sleep disorder assessment: a secondary analysis of health administrative data
Authors: Kendzerska Tetyana , Malhotra Atul , Gershon Andrea S. , Povitz Marcus , McIsaac Daniel I. , Aaron Shawn D. , Bryson Gregory L. , Talarico Robert , Godbout Michael , Tanuseputro Peter , Chung Frances 
Published: July 2023

BackgroundInformation is needed to guide safe opioid prescribing in adults referred for a sleep disorder assessment. Previous studies have shown that individuals referred for a sleep disorder assessment have a higher likelihood of long-acting opioids and higher opioid dosages prescription than the general population, suggesting that these individuals are more at risk for opioid-related adverse health consequences.MethodsWe included all adults who underwent a diagnostic sleep study (index date) in Ontario, Canada, between 2013 and 2016 (n = 300,663) and filled an opioid prescription overlapping the index date (n = 15,713). Through provincial health administrative databases, individuals were followed over time to assess the association between opioid use characteristics and 1-year all-cause mortality, hospitalizations and emergency department (ED) visits, and opioid-related hospitalizations and ED visits within extended follow-up to 2018.ResultsControlling for covariates, chronic opioid use (vs. not) was significantly associated with increased hazards of all-cause mortality [adjusted hazard ratio(aHR): 1.84; 95% confidence interval (CI): 1.12–3.02], hospitalization (aHR: 1.14; 95% CI: 1.02–1.28) and ED visit (aHR: 1.09; 95% CI: 1.01–1.17). A higher opioid dosage [morphine equivalent daily dose (MED) &gt;90 vs. ≤ 90 mg/day] was significantly associated with increased hazards of all-cause or opioid-related hospitalization (aHR: 1.13; 95% CI: 1.02–1.26 and aHR: 2.27; 95% CI: 1.53–3.37, respectively). Morphine or hydromorphone prescription (vs. oxycodone) was significantly associated with an increased hazard of all-cause hospitalization (aHR: 1.30; 1.07–1.59 and aHR: 1.43; 95% CI: 1.20–1.70, respectively). Hydromorphone or fentanyl prescription (vs. oxycodone) was significantly associated with an increased hazard of opioid-related ED visit and/or hospitalization (aHR: 2.28, 95% CI: 1.16–4.47 and aHR: 2.47, 95% CI: 1.16–5.26, respectively).ConclusionFindings from this retrospective study may inform the safe prescribing of opioids in adults referred for a sleep disorder assessment.
Effects of Different Submerged Macrophytes on the Water and Sediment in Aquaculture Ponds with Enrofloxacin Residues
Authors: Zhang Lingling , Liu Lizao , Zhang Yuping 
Published: July 2023

Submerged macrophyes have been widely used to restore aquaculture ponds in recent years. Yet, whether the residual antibiotics in ponds will affect the remediation effect of submerged macrophyes, and the effect of different submerged macrophyes on the water and sediment in aquaculture ponds with antibiotic residues, is unclear. A microcosm experiment was carried out to study the interaction between three kinds of submerged macrophytes and their growing environment with antibiotic residues. Ceratophyllum demersum L. with no roots, Vallisneria spiralis L. with flourish roots, and Hydrilla verticillata L with little roots were chosen to be planted in the sediment added with enrofloxacin (ENR). The growth of submerged macrophytes, the changes of the overlying water and sediment characteristics, and the microbial community in the sediment were analyzed. The results showed that according to the growth rate and nutrients accumulation ability, V. spiralis with flourish roots performed best among the three submerged macrophytes. The concentrations of TOC, TP, NH4+-N, and TN in the overlying water were 25.0%, 71.7%, 38.1%, and 24.8% lower in the V. spiralis treatment comparing with the control, respectively. The richness and diversity of the microorganisms in the sediment of V. spiralis treatment were significantly higher than those in the control, but this advantage was not obvious in the H. verticillata treatment. V. spiralis promoted the growth of Proteobacteria (22.8%) and inhibited the growth of Acidobacteria (32.1%) and Chloflexi (31.7%) in the rhizosphere sediment with ENR residue. The effects of the three submerged macrophytes on the removal of ENR from sediment were not reflected due to the limitation of water depth. Compared with C. demersum and H. verticillata, V. spiralis was more suitable for the remediation of the aquaculture ponds with ENR residue.
Impact of sociodemographic status and UTI symptoms on women’s health-care seeking and management in England: findings from an e-survey conducted during the first year of the COVID-19 pandemic
Authors: Cooper Emily , Read Brieze , Sanyaolu Leigh , Ahmed Haroon , Lecky Donna 
Published: July 2023

BackgroundMultiple factors may influence women’s experiences of urinary tract infection (UTI) and its clinical management.AimTo explore how women’s background, symptoms, and severity of symptoms influence UTI reporting and management.Design & settingInternet questionnaire targeting women in England, focusing on UTI symptoms, care seeking, and management.MethodA total of 1096 women aged ≥16 years with UTI symptoms in the previous year completed the questionnaire in March and April 2021. Multivariable logistic regression was ued to estimate the odds of relevant outcomes while adjusting for background characteristics.ResultsWomen with children in their household, who were aged under 45 years, or who were married or cohabitating were more likely to experience UTI symptoms. The odds of antibiotic prescribing were lower if women reported dysuria (adjusted odds ratio [AOR] = 0.65, 95% confidence interval [CI] = 0.49 to 0.85), frequency (AOR = 0.63, 95% CI = 0.48 to 0.83), or vaginal discharge (AOR = 0.69, 95% CI = 0.50 to 0.96), but higher if reporting haematuria (AOR = 2.81, 95% CI = 1.79 to 4.41), confusion (AOR = 2.14, 95% CI = 1.16 to 3.94), abdominal pain (AOR = 1.35, 95% CI = 1.04 to 1.74), or systemic symptoms (AOR = 2.04, 95% CI = 1.56 to 2.69). Those with abdominal pain or two or more of nocturia, dysuria, or cloudy urine had lower odds of receiving a delayed antibiotic, while those with incontinence, confusion, unsteadiness, or low temperature had higher odds of a delayed prescription. Increasing symptom severity was associated with greater odds of receiving antibiotics.ConclusionExcept for reduced prescribing if a woman had dysuria and frequency, antibiotic prescribing followed an expected pattern, aligning generally with national guidance. Symptom severity and the likelihood of systemic infection probably influenced care seeking and prescribing. Sexual intercourse and childbirth may be key times to target women with messages about UTI prevention.