bluuebunny/crossref_metadata_2025_split huggingface dataset:
Physicochemical analysis as food safety in pasteurized milk in CV. Cita Nasional
Authors: Aji HP , Nuhriawangsa AMP , Kartikasari LR 
Published: June 2023

Abstract
Milk is an animal-source food that has a high nutritional with contains 2.8% protein, 3.0% fat, vitamin A, B, C, D, and others. CV. Cita Nasional is one of the dairy processing industries that produces pasteurized milk products. The purpose of this study was to analyze food safety by conducting physicochemical testing of pasteurized milk products with the types HTST (High-Temperature Short Time) or LTLT (Low-Temperature Long Time). The research method used was physicochemical testing on the finished product of pasteurized milk including pH, Fat, Total Solid, Brix and Organoleptic. Physicochemical testing was done by comparing the finished product data in CV. Cita Nasional with the Indonesian National Standard (SNI) data. The Mann-Whitney Test was used for the data analysis. The results showed a significant value for the variables of temperature 0.565; pH 0.104; Fat 0.001; Total Solid 0.000 and brix 0.000. Based on the data analysis results, the overall significance value was <0.05, which means that the Mann-Whitney Test showed a positive effect on the physicochemical quality and that the physicochemical test of pasteurized milk products meets the requirements of SNI.
Description of patient questions received by clinical pharmacists in the Nudge Study
Authors: Marrs Joel C , Orlando Steven T , Saseen Joseph J , Novins-Montague Sylvie , Sandy Lisa Caputo , Waughtal Joy , Glorioso Thomas J , Ho P Michael 
Published: June 2023

Abstract

Purpose
The Nudge Study is a patient level–randomized trial testing different text message medication refill reminders sent to patients assigned to 4 arms: (1) usual care, (2) generic text, (3) optimized text, and (4) optimized text plus chatbot. This report describes the frequency and types of patient questions sent to clinical pharmacists (CPs) following text reminders.

Methods
Patients were enrolled from Denver Health and Hospital Authority (DHHA) and Veterans Affairs Eastern Colorado Health Care System (VA ECHCS) from October 1, 2019, through May 30, 2021. Included patients responded to at least 1 text or interactive voice response (IVR) message. Patients were dichotomized as those who posed at least 1 question to a CP and those who posed no questions.

Results
Of the 6,325 patients enrolled in an intervention arm, 3,323 (52.5%) responded to at least 1 text or IVR message, and among those responding, 305 (9.2%) responded with a pharmacist question. Patient factors associated with submitting a CP question included age (45-74 years), enrollment from DHHA, and receipt of the optimized text or optimized text plus chatbot message versus the generic text. Questions to CP were in the following categories: medication related (48.2%), refill logistics (38.4%), cost (9.2%), and other (17.7%).

Conclusion
In a text messaging intervention focused on medication refills, there were few questions directed to the CP. Patients assigned to receive optimized texts were more likely to have questions. We hypothesize that this may suggest greater patient engagement regarding their condition, resulting in more questions.
Facial Hypertrophy as a Complication of Weight Gain in Autologous Fat Graft Patients: Considerations and Recommendations
Authors: Anderson Lexy , Nguyen Clara Do Tran , Trinh Kathleen , Dorfman Robert , Tandon Vickram , Do Nicholas , Lambros Val , Grotting James , Song David , Delong Michael 
Published: June 2023

Abstract

Fat grafting can restore facial volume for reconstructive and cosmetic indications. Common practice often involves extracting lipoaspirate from the most abundant anatomic location. However, grafted fat retains the phenotypic characteristics of its original location and can undergo exaggerated hypertrophy with patient weight fluctuations. The aim of this study was to systematically assess the literature to summarize the reported effects of postoperative weight gain on facial hypertrophy in patients after facial fat grafting and to determine potentially avoidable factors. A search through PubMed/MEDLINE was conducted on October 4, 2022, to identify relevant articles with appropriate search terms. No lower date limit was applied and all eligible nonanimal clinical articles in English were included for review. Reports were summarized and presented as descriptive statistics. The search generated 714 articles. After abstract and full-text review of the initial set of articles, 6 were included in our analysis. All articles described poor cosmetic outcomes resulting from nonanatomic hypertrophy of the grafted fat. None of the articles reported a thorough methodology for selecting the donor site to minimize fat hypertrophy with potential future weight fluctuations. Grafted facial fat is susceptible to exaggerated hypertrophy as a result of changes in patient weight. Specifically, harvesting lipoaspirate from maximally abundant areas of the body may increase this risk. Individualizing the area of fat donation may attenuate unwanted fat growth and further contribute to increased patient quality of life.

Level of Evidence: 4
Man, or Machine? Artificial Intelligence Language Systems in Plastic Surgery
Authors: Palacios Jose F , Bastidas Nicholas 
Published: June 2023

Abstract
Artificial intelligence (AI) language models are computer programs trained to understand and generate human-like text. The latest AI language models available to the public have impressive language generation capability with immediate applications in both academia and private practice. Plastic surgeons can immediately leverage this technology to more efficiently allocate valuable human capital to higher-yield tasks. This can ultimately translate to higher patient volume, higher research output, and improved patient communication. Commercially available models offer business solutions that should not be ignored by plastic surgeons hoping to establish, optimize, or grow their practices. In this paper, the authors review the current state of AI language systems, discuss potential applications, and explore the risks and limitations of this technology.
Farrerol attenuates glutamate-induced apoptosis in HT22 cells via the Nrf2/heme oxygenase-1 pathway
Authors: Gao Liying , Wang Tong , Zhuoma Dongzhi , Yuan Ruiying , Huang Shan , Li Bin 
Published: June 2023

ABSTRACTFarrerol is a flavonoid found in plants with a wide range of pharmacological effects, including protection and enhancement of nerve cell function, as well as antioxidant and antibacterial properties, among others. Neurodegenerative diseases are irreversible neurological disorders resulting from the loss of neuronal cells in the brain and spinal cord. In this experiment, we investigated the neuroprotective and antioxidant effects of farrerol on glutamate-induced HT22 cells. Our results showed that farrerol inhibited reactive oxygen species expression, apoptosis, mitochondrial damage, and the activation of caspases 3 and 9 in HT22 cells induced by glutamate. Additionally, farrerol potentially regulated the Nrf2/heme oxygenase-1 (HO-1) signaling pathway, as it attenuated the nuclear translocation of Nrf2 and promoted the expression of HO-1. These findings suggest that farrerol has potential as a new therapeutic option.
Genetic fine-mapping from summary data using a nonlocal prior improves the detection of multiple causal variants
Authors: Karhunen Ville , Launonen Ilkka , Järvelin Marjo-Riitta , Sebert Sylvain , Sillanpää Mikko J 
Published: June 2023

AbstractMotivationGenome-wide association studies (GWAS) have been successful in identifying genomic loci associated with complex traits. Genetic fine-mapping aims to detect independent causal variants from the GWAS-identified loci, adjusting for linkage disequilibrium patterns.ResultsWe present “FiniMOM” (fine-mapping using a product inverse-moment prior), a novel Bayesian fine-mapping method for summarized genetic associations. For causal effects, the method uses a nonlocal inverse-moment prior, which is a natural prior distribution to model non-null effects in finite samples. A beta-binomial prior is set for the number of causal variants, with a parameterization that can be used to control for potential misspecifications in the linkage disequilibrium reference. The results of simulations studies aimed to mimic a typical GWAS on circulating protein levels show improved credible set coverage and power of the proposed method over current state-of-the-art fine-mapping method SuSiE, especially in the case of multiple causal variants within a locus.Availability and implementationhttps://vkarhune.github.io/finimom/.
Plasmid permissiveness of wastewater microbiomes can be predicted from 16S rRNA sequences by machine learning
Authors: Moradigaravand Danesh , Li Liguan , Dechesne Arnaud , Nesme Joseph , de la Cruz Roberto , Ahmad Huda , Banzhaf Manuel , Sørensen Søren J , Smets Barth F , Kreft Jan-Ulrich 
Published: June 2023

Abstract

Motivation
Wastewater treatment plants (WWTPs) harbor a dense and diverse microbial community. They constantly receive antimicrobial residues and resistant strains, and therefore provide conditions for horizontal gene transfer (HGT) of antimicrobial resistance (AMR) determinants. This facilitates the transmission of clinically important genes between, e.g. enteric and environmental bacteria, and vice versa. Despite the clinical importance, tools for predicting HGT remain underdeveloped.

Results
In this study, we examined to which extent water cycle microbial community composition, as inferred by partial 16S rRNA gene sequences, can predict plasmid permissiveness, i.e. the ability of cells to receive a plasmid through conjugation, based on data from standardized filter mating assays using fluorescent bio-reporter plasmids. We leveraged a range of machine learning models for predicting the permissiveness for each taxon in the community, representing the range of hosts a plasmid is able to transfer to, for three broad host-range resistance IncP plasmids (pKJK5, pB10, and RP4). Our results indicate that the predicted permissiveness from the best performing model (random forest) showed a moderate-to-strong average correlation of 0.49 for pB10 [95% confidence interval (CI): 0.44–0.55], 0.43 for pKJK5 (0.95% CI: 0.41–0.49), and 0.53 for RP4 (0.95% CI: 0.48–0.57) with the experimental permissiveness in the unseen test dataset. Predictive phylogenetic signals occurred despite the broad host-range nature of these plasmids. Our results provide a framework that contributes to the assessment of the risk of AMR pollution in wastewater systems.

Availability and implementation
The predictive tool is available as an application at https://github.com/DaneshMoradigaravand/PlasmidPerm.
Faithfully Describing and Responding to Addiction and Pain: Christian “Homefulness” and Desire
Authors: Swinton John , Yang Emmy 
Published: June 2023

Abstract
This investigation develops in three steps. First, we seek to complexify the opioid crisis in a way that helps us to see how the issues of misguided desire and misplaced attachments are fundamentally important for a theological account of opioid addiction.1 Second, acknowledging the connections between pain and opioid addiction, we explore some of the ways in which our understanding of pain can influence our understanding of and responses to opioid use. Finally, we offer some tentative reflections on the theological and ecclesial dimensions of the opioid crisis which hold the potential to influence the nature of a Christian response to the issues. Having laid out these steps, we argue for the church’s calling, where she complexifies the narratives about the opioid crisis and provides individuals suffering from opioid use “homeful” encounters with God and the community.
Efficacy and safety of hypoxia-inducible factor prolyl hydroxylase inhibitors in patients with chronic kidney disease: meta-analysis of phase 3 randomized controlled trials
Authors: Minutolo Roberto , Liberti Maria Elena , Simeon Vittorio , Sasso Ferdinando C , Borrelli Silvio , De Nicola Luca , Garofalo Carlo 
Published: June 2023

ABSTRACT

Background
Hypoxia-inducible factor prolyl hydroxylase inhibitors (HIF-PHIs) are new therapeutic agents for anaemia in chronic kidney disease (CKD). We evaluated by meta-analysis and meta-regression the efficacy and safety of HIF-PHIs in patients with CKD-related anaemia.

Methods
We selected phase 3 randomized clinical trials (RCTs) comparing HIF-PHIs and erythropoiesis-stimulating agents (ESAs) in dialysis and non-dialysis patients. Efficacy outcomes were the changes from baseline of haemoglobin, iron parameters (hepcidin, serum iron, TIBC, TSAT, ferritin) and intravenous iron dose; as safety outcomes we considered cancer, adjudicated major adverse cardiovascular events (MACE), MACE+ (MACE plus hospitalization for hearth failure or unstable angina or thromboembolic event), thrombotic events (deep vein thrombosis, pulmonary embolism), arterovenous fistula (AVF) thrombosis and death.

Results
We included 26 RCTs with 24 387 patients. Random effect meta-analysis of the unstandardized mean difference between HIF-PHIs and ESAs showed a significant change in haemoglobin levels from baseline of 0.10 g/dL (95% CI 0.02 to 0.17). Meta-regression analysis showed a significantly higher haemoglobin change for HIF-PHIs in younger patients and versus short-acting ESA (0.21 g/dL, 95% CI 0.12 to 0.29 versus –0.01, 95% CI –0.09 to 0.07 in studies using long-acting ESA, P &lt; .001). No significant effect on heterogeneity was found for type of HIF-PHIs. In comparison with ESAs, HIF-PHIs induced a significant decline in hepcidin and ferritin and a significant increase in serum iron and TIBC, while TSAT did not change; intravenous iron dose was lower with HIF-PHI (–3.1 mg/week, 95% CI –5.6 to –0.6, P = .020). Rate ratio of cancer (0.93, 95% CI 0.76 to 1.13), MACE (1.00, 95% CI 0.94 to 1.07), MACE+ (1.01, 95% CI 0.95 to 1.06), thrombotic events (1.08, 95% CI 0.84 to 1.38), AVF thrombosis (1.02, 95% CI 0.93 to 1.13) and death (1.02, 95% CI 0.95 to 1.13) did not differ between HIF-PHIs and ESAs.

Conclusions
HIF-PHIs at the doses selected for the comparisons are effective in correcting anaemia in comparison with ESA therapy with a significant impact on iron metabolism without notable difference among various agents. No safety signals emerge with use of HIF-PHIs.
NephroCheck at 10: addressing unmet needs in AKI diagnosis and risk stratification
Authors: Martin-Cleary Catalina , Sanz Ana Belen , Avello Alejandro , Sanchez-Niño Maria Dolores , Ortiz Alberto 
Published: June 2023

ABSTRACT
Despite its name, the current diagnosis of acute kidney injury (AKI) still depends on markers of decreased kidney function and not on markers of injury. This results in a delayed diagnosis: AKI is diagnosed based on serum creatinine criteria only when the severity of injury is enough to decrease glomerular filtration rate. Moreover, by the time AKI is diagnosed, the insult may have already ceased, and even appropriate therapy targeted at the specific insult and its associated pathogenic pathways may no longer be effective. Biomarkers of injury are needed that allow the diagnosis of AKI based on injury criteria. At least three commercially available immunoassays assessing urinary or plasma neutrophil gelatinase-associated lipocalin and urinary tissue inhibitor of metalloproteinases-2 × insulin-like growth factor-binding protein-7 ([TIMP2]*[IGFBP7]) (NephroCheck®) have generated promising data regarding prediction and early diagnosis of AKI, although their relative performance may depend on clinical context. Recently, a urinary peptidomics classifier (PeptAKI) was reported to predict AKI better than current biomarkers. Focusing on [TIMP2]*[IGFBP7], the cellular origin of urinary TIMP2 and IGFBP7 remains unclear, especially under the most common predisposing condition for AKI, i.e. chronic kidney disease. We now discuss novel data on the kidney cell expression of TIMP2 and IGFBP7 and its clinical implications.
Aetiology and outcome in hospitalized cardiac arrest patients
Authors: Albert Malin , Herlitz Johan , Rawshani Araz , Forsberg Sune , Ringh Mattias , Hollenberg Jacob , Claesson Andreas , Thuccani Meena , Lundgren Peter , Jonsson Martin , Nordberg Per 
Published: June 2023

Abstract

Aims
To study aetiologies of in-hospital cardiac arrests (IHCAs) and their association with 30-day survival.

Methods and results
Observational study with data from national registries. Specific aetiologies (n = 22) of IHCA patients between April 2018 and December 2020 were categorized into cardiac vs. non-cardiac and six main aetiology categories: myocardial ischemia, other cardiac causes, pulmonary causes, infection, haemorrhage, and other non-cardiac causes. Main endpoints were proportions in each aetiology, 30-day survival, and favourable neurological outcome (Cerebral Performance Category scale 1–2) at discharge. Among, 4320 included IHCA patients (median age 74 years, 63.1% were men), approximate 50% had cardiac causes with a 30-day survival of 48.4% compared to 18.7% among non-cardiac causes (P &lt; 0.001). The proportion in each category were: myocardial ischemia 29.9%, pulmonary 21.4%, other cardiac causes 19.6%, other non-cardiac causes 11.6%, infection 9%, and haemorrhage 8.5%. The odds ratio (OR) for 30-day survival compared to myocardial ischemia for each category were: other cardiac causes OR 1.48 (CI 1.24–1.76); pulmonary causes OR 0.36 (CI 0.3–0.44); infection OR 0.25 (CI 0.18–0.33); haemorrhage OR 0.22 (CI 0.16–0.3); and other non-cardiac causes OR 0.56 (CI 0.45–0.69). IHCA caused by myocardial ischemia had the best favourable neurological outcome while those caused by infection had the lowest OR 0.06 (CI 0.03–0.13).

Conclusion
In this nationwide observational study, aetiologies with cardiac and non-cardiac causes of IHCA were evenly distributed. IHCA caused by myocardial ischemia and other cardiac causes had the strongest associations with 30-day survival and neurological outcome.
Metabolic Responses of Normal Rat Kidneys to a High Salt Intake
Authors: Shimada Satoshi , Hoffmann Brian R , Yang Chun , Kurth Theresa , Greene Andrew S , Liang Mingyu , Dash Ranjan K , Cowley Allen W 
Published: June 2023

Abstract
In this study, novel methods were developed, which allowed continuous (24/7) measurement of arterial blood pressure and renal blood flow in freely moving rats and the intermittent collection of arterial and renal venous blood to estimate kidney metabolic fluxes of O2 and metabolites. Specifically, the study determined the effects of a high salt (HS; 4.0% NaCl) diet upon whole kidney O2 consumption and arterial and renal venous plasma metabolomic profiles of normal Sprague–Dawley rats. A separate group of rats was studied to determine changes in the cortex and outer medulla tissue metabolomic and mRNAseq profiles before and following the switch from a 0.4% to 4.0% NaCl diet. In addition, targeted mRNA expression analysis of cortical segments was performed. Significant changes in the metabolomic and transcriptomic profiles occurred with feeding of the HS diet. A progressive increase of kidney O2 consumption was found despite a reduction in expression of most of the mRNA encoding enzymes of TCA cycle. A novel finding was the increased expression of glycolysis-related genes in Cx and isolated proximal tubular segments in response to an HS diet, consistent with increased release of pyruvate and lactate from the kidney to the renal venous blood. Data suggests that aerobic glycolysis (eg, Warburg effect) may contribute to energy production under these circumstances. The study provides evidence that kidney metabolism responds to an HS diet enabling enhanced energy production while protecting from oxidative stress and injury.
Metabolomic and transcriptomic analysis of kidneys of Sprague-Dawley rats fed a high salt diet.
Concluding Embryogenesis After Diaspora: Seed Germination in Illicium Parviflorum
Authors: Losada Juan M 
Published: June 2023

Synopsis
Albuminous seeds, dispersed with a minimally developed embryo surrounded by nutrient storage tissue, are pervasive across extinct and extant early diverging angiosperm lineages. Typically, seed ontogenic studies have focused on the time between fertilization and seed release, but in albuminous seeds, embryogenesis is incomplete at the time of seed dispersal. Here, I studied the morphological and nutritional relationships between the embryo and the endosperm after seed dispersal in Illicium parviflorum (Austrobaileyales). Seeds of I. parviflorum germinate over a period of three months. Different stages during the germination process were anatomically evaluated using a combination of histochemistry and immunocytochemistry. At dispersal, the seeds of Illicium contain a tiny achlorophyllous embryo with minimal histological differentiation, surrounded by copious amounts of lipo-protein globules stored in the endosperm within cell walls rich in un-esterified pectins. Six weeks later, the embryo expanded and differentiated the vascular tissues before the emergence of the radicle through the seed coat, as the stored lipids and proteins coalesced within cells. Six weeks later, the cotyledons contained starch and complex lipids intracellularly, and accumulated low-esterified pectins in their cell walls. The proteolipid-rich albuminous seeds of Illicium exemplify how woody angiosperms of the Austrobaileyales, Amborellales, and many magnoliids release seeds with high-energy storage compounds that are reprocessed by embryos that complete development during germination. Seedlings of these lineages thrive in the understory of tropical environments, which match with the predicted habitats where angiosperms evolved.
Effect of papillary muscle approximation on acute ovine functional tricuspid regurgitation
Authors: Gaweda Boguslaw , Jaźwiec Tomasz , Gaddam Manikantam , Bush Jared D , MacDougall Brian , Widenka Kazimierz , Timek Tomasz A 
Published: June 2023

Abstract

OBJECTIVES
Ring annuloplasty represents the standard surgical treatment, but offers suboptimal results in patients with severe functional tricuspid regurgitation. Addition of papillary muscles (PMs) approximation may improve clinical outcomes.

METHODS
Eight healthy adult male sheep (56 ± 4 kg) underwent cardiopulmonary bypass and implantation of sonomicrometry crystals on the tricuspid annulus, PM tips and right ventricular (RV) free wall. Papillary muscles approximation sutures were anchored between anterior–posterior and anterior–septal PMs and their loose ends externalized through RV free wall to epicardial tourniquets. After weaning from cardiopulmonary bypass, acute right heart failure and tricuspid regurgitation were induced, and subsequent sequential anterior–posterior and anterior–septal PM approximations were performed. Echocardiographic, haemodynamic and sonomicrometry data were collected.

RESULTS
Tricuspid regurgitation at baseline in eight sheep was none or trace in 3 and mild in 5, and after induction of acute right heart failure increased significantly to moderate in 5, moderately severe in 1 and severe in 2 (P = 0.011). RV pressure increased from 31 [28; 43] to 51 [47; 55] mmHg (P = 0.012). Anterior–posterior PM approximation decreased regurgitation grade to none or trace in 1, mild in 4 and moderate in 3 (P = 0.016) and reduced PM area from 208 [160; 241] to 108 [48; 181] mm2 (P = 0.008), and anterior–posterior PM distance from 18 [16; 20] to 10 [7; 13] mm (P = 0.037). Anterior–septal approximation also significantly reduced PM area but had no effect on regurgitation grade.

CONCLUSIONS
Anterior–posterior but not anterior–septal PM approximation alleviated acute ovine tricuspid regurgitation. Selective PM approximation may offer better control of tricuspid regurgitation.