bluuebunny/crossref_metadata_2025_split huggingface dataset:
Causes & risk factors for unscheduled readmissions in the paediatric ward of a major public hospital
Authors: Kundana P. Keerthi , Agrawal Mukesh , Tullu Milind S. , Kulkarni Ankita A. 
Published: April 2023

Background & objectives:
Readmissions are often considered as an indicator of poor quality of care during previous hospitalization, although many of these are unavoidable or unrelated to the past admission. The identification of high-risk cases for readmissions and appropriate interventions will help not only reduce the hospital burden but also to establish the credibility of the hospital. So this study aimed to determine the readmission percentage in the paediatric wards of a tertiary care hospital and to identify the reasons and risk factors that can help minimize preventable re-hospitalizations.

Methods:
This prospective study from a public hospital included 563 hospitalized children, classified as first admission or readmissions. Readmissions were defined as one or more hospitalizations within preceding six months, excluding scheduled admissions for investigations or treatment. Reason-wise, the readmissions were classified into various categories, based on the opinion of three paediatricians.

Results:
The percentage of children getting readmitted within six, three and one month time from the index admission was 18.8, 11.1 and 6.4 per cent, respectively. Among readmissions, 61.2 per cent were disease-related, 16.5 per cent unrelated, 15.5 per cent patient-related, 3.8 per cent medication/procedure-related and 2.9 per cent physician-related causes. Patient- and physician-related causes were deemed preventable, contributing to 18.4 per cent. The proximity of residence, undernutrition, poor education of the caretaker and non-infectious diseases were associated with increased risk of readmission.

Interpretation & conclusions:
The findings of this study suggest that readmissions pose a substantial burden on the hospital services. The primary disease process and certain sociodemographic factors are the major determinants for the increased risk of readmissions among paediatric patients.
High-risk human papillomavirus in Turkish patients with clinically suspicious cervical lesions analyzed by multiplex-PCR
Authors: Gorur Levent , Dolanbay Mehmet , Ozturk Figen , Canoz Ozlem , Donmez-Altuntas Hamiyet 
Published: April 2023

Background & objectives:
Human papillomavirus (HPV) infection is known to be the main cause of cervical cancer. This study aimed to determine the prevalence of high-risk HPV genotypes in smear specimens taken from women who had normal or abnormal cytology using a multiplex PCR method.

Methods:
The study included 270 women aged between 19 and 69 yr with or without suspicious cervical abnormalities. A Pap smear sample from each patient was cytologically examined, and HPV typing was performed using a multiplex fluorescent PCR method. Those who were high-risk HPV positive and had a normal or abnormal cytology were further evaluated by colposcopy and biopsy.

Results:
The total HPV positivity was 43 per cent (116/270). HPV positivity in the patients with an abnormal cytology was 77 per cent (33/43), whereas it was only 37 per cent (83/227) in women with normal cytology, which showed a significant difference (P<0.05). HPV positivity was also related to the age group when all the subjects were considered (P<0.05), and the highest prevalence of HPV infection was in the 30-39 yr age group. High-risk HPV types 16, 18, 31, 35, 51 and 56 were more common in the normal cytology patients, whereas high-risk HPV types 16, 31, 35, 45, 58 and 68 were commonly found in the abnormal cytology patients.

Interpretation & conclusions:
The determination of high-risk HPV genotypes in women with clinically suspicious cervical lesions should be conducted during an annual follow-up, irrespective of a normal or abnormal cytology by the age of 30 years or above.
Konstruk Ideologi Muhammadiyah: Fondasi Pengembangan Instrumen Pengukuran Kekuatan Ideologi Muhammadiyah
Authors: Subando Joko , Samsuri Muh , Muslimin Edy 
Published: April 2023

This study aims to find out the construct of Muhammadiyah ideology. This research was categorized as qualitative research with literature review as a method of data collection. The data consisted of the concept and the understanding related to Muhammadiyah ideology, the source of data was books and journals examining Muhammadiyah ideology from the aspect of outlook on life, life guidelines of Muhammadiyah citizens and Muhammadiyah strategies in realizing their ideals of life. Data analysis techniques used a simplified approach. The results show that the Muhamadiyah Ideology is a set of understandings that contain muhammadiyah views, life guidelines and strategies in realizing his ideals of life. Muhammadiyah's view of life is reflected in the muqaddimah of Muhammadiyah's articles of association containing views on anchorage, community, views on Islamic teachings, enforcement of Islamic teachings, ittiba' nabi in struggle, views of organization and muhammadiyah ideals. The life guidelines of Muhammadiyah residents discuss self-guidelines in religion, socializing in society, organizing and guidelines for state life. There are several Muhammadiyah strategies in realizing their life ideals pursued by community development related to aqidah, worship and morals, improving the quality of life of the community, fostering Muhammadiyah cadres, strengthening Muhammadiyah associations and appropriate political and state strategies. Furthermore, the ideological construct can be used as a foundation in the development of instruments for measuring the strength of Muhammadiyah ideology.
Waarom het maar niet wil lukken…
Authors: Bos Eltje 
Published: April 2023

Cultural policy and immigrants. Why did the integration fail?
In the aftermath of the last millennium a vice minister intended to realise what his predecessors of almost 25 years planned but never accomplished. The cultural expressions of immigrants were supposed to become integrated in the general cultural policy and the subsidized cultural organisations. The article explores why the policies never were formulated and implemented as the various (vice-)ministers explicitly intended.The main reason seems to be the dominance of the romantic universal interpretation of the notion ‘artistic quality’. The mentioned vice minister explicitly challenged this dominance, but didn’t succeed.Subsidies in the cultural sector were and are mainly allocated because of the artistic quality of cultural expressions. The majority of the key actors in this sector such as advisors, cultural institutions, politicians tend to have a romantic universal perspective on quality. These actors seemed to be unwilling or unable to broaden up their conception of quality. Due to this special implementation arrangements were created for the cultural expressions of immigrants. This implied the integration never was accomplished, and over time both cultural institutions and immigrant initiatives seemed relatively content with these arrangements.
Agents Isolated from Vaginal Cultures in the Reproductive Period and Their Antibiotic Sensitivities (Vaginal Culture and Antibiotic Sensitivitie)
Authors: Dinç Kemal , Akyüz Sümeyye 
Published: April 2023

Introduction: In our study, we aimed to examine the strains isolated from vaginal swab samples sent to our laboratory from various clinics with a pre-diagnosis of vulvovaginitis and antibiotic resistance rates, retrospectively.
Methods: ethods: 90 vaginal swab samples of patientswere included into this study. Two samples were taken from each patient and one sample was used for Gram staining. The other sample was inoculated in 5% sheep blood, Eozine Methylene Blue agar and Saboraud dextrose agar. TIdentification of isolated colonies and antibiotic susceptibility studies were carried out with Vitek 2 Compact automated system. Result of the susceptibility testing are reported according to EUCAST criteria. Nugent scoring was used for bacterial vaginosis.
Results: Normal vaginal flora elements were found in 66 (73.3 %) of the specimens, and 28 organisms were found in 24 (26.7 %). The distribution of the organisms is as follows: 6 (21.4%) Escherichia coli, 5(17.9%) Streptococcus agalactiae, 5 (17.9%) Gardnerella vaginalis, 3 (10.7%) Candida spp., 3(10.7%) Klebsiella pneumoniae, 3(10.7%) Enterococcus faecalis. Two of the E.coli strains and one of the K.pneumoniae strains are ESBL positive. Of the agents, 21 were isolated from outpatients and 7 from inpatients. All S. agalactiae strains were susceptible to penicillin and ampicillin. E.coli and K.pneumoniae strains, which are the most frequently isolated Gram (-) organisms, are most susceptible to amikacin, carbapenems and tigecycline.
Conclusion: In patients with vaginal discharge and itching complaints, determining the organisms with microbiological culture and oriented treatment instead of empirical treatment will be more beneficial for cure.
PENGGUNAAN APLIKASI ADOBE ANIMATE CC DALAM MENDESAIN MULTIMEDIA INTERAKTIF BERBASIS KONFLIK KOGNITIF MATERI ALAT-ALAT OPTIK
Authors: Ina Felina Ardini , Mufit Fatni 
Published: April 2023

Beberapa penelitian menunjukan miskonsepsi terjadi pada materi alat-alat optik. Penggunaan multimedia interaktif juga kurang dalam mendukung pembelajaran abad 21. Penelitian ini bertujuan untuk menghasilkan disain multimedia interaktif berbasis konflik kognitif menggunakan adobe animate cc materi alat-alat optik yang valid. Jenis penelitian adalah penelitian pengembangan (development/design research) menggunakan model Plomp. Tahap penelitian ini meliputi preliminary research dan develop or prototyping phase yang dibatasi sampai expert review evaluation. Sumber data diperoleh dari hasil angket, analisis jurnal dan validasi tenaga ahli oleh 3 dosen Fisika. Teknik analisis data menggunakan persentasi dan formula V Aiken. Berdasarkan preliminary research diperoleh pemahaman konsep siswa rendah pada materi alat – alat optik, belum tersedia bahan ajar berbasis IT yang dapat meningkatkan pemahamn konsep. Pada Develop or Prototyping Phase telah didesain multimedia interaktif sesuai dengan sintaks model pembelajaran berbasis konflik kognitif. Hasil self-evaluation diperoleh dengan kriteria sangat baik. Hasil uji validitas diperoleh rata-rata 0,86 dengan kategori sangat valid. Jadi, disimpukan bahwa multimedia interaktif berbasis konflik kognitif terutama pada materi alat-alat optik sangat valid dengan aspek substansi materi, desain materi, desain pembelajaran, tampilan komunikasi visual dan penggunaan software.
Supplemental data from An Essential Role of <i>Maspin</i> in Embryogenesis and Tumor Suppression
Authors: Dzinic Sijana H. , Bernardo M. Margarida , Li Xiaohua , Fernandez-Valdivia Rodrigo , Ho Ye-Shih , Mi Qing-Sheng , Bandyopadhyay Sudeshna , Lonardo Fulvio , Vranic Semir , Oliveira Daniel S.M. , Bonfil R. Daniel , Dyson Gregory , Chen Kang , Omerovic Almasa , Sheng Xiujie , Han Xiang , Wu Dinghong , Bi Xinling , Cabaravdic Dzenana , Jakupovic Una , Wahba Marian , Pang Aaron , Harajli Deanna , Sakr Wael A. , Sheng Shijie 
Published: April 2023

<p>List of supplemental materials accompanying the current manuscript: 1.	Table S1. List of primers and expected amplicon sizes for genotyping and mRNA detection. 2.	Table S2. Live births & gender distribution. 3.	Figure S1. Tertiary maspin structure. 4.	Figure S2. qRT-PCR detection of mRNA in mouse mammary gland and small intestine tissues using primers PB1687/1688. 5.	Figure S3. Identification of PrPC mRNA. 6.	Figure S4. Histopathological (H&E) and immunohistological (IHC) analysis of mouse tissues. 7.	Figure S5. Mouse growth analysis. 8.	Figure S6. Histopathological analysis of mouse testes and ovaries. 9.	Figure S7. Mammary gland adenoma of mouse KO tissue. 10.	Figure S8. Histomorphometry of wt/wt mouse prostate. 11.	Figure S9. Histopathological analysis of mouse small intestine tissues.</p>
Figure S3 from Epstein–Barr Virus miRNA BART2-5p Promotes Metastasis of Nasopharyngeal Carcinoma by Suppressing RND3
Authors: Jiang Chen , Li Lei , Xiang Yan-Qun , Lung Maria Li , Zeng Tingting , Lu Jiabin , Tsao Sai Wah , Zeng Mu-Sheng , Yun Jing-Ping , Kwong Dora L.W. , Guan Xin-Yuan 
Published: April 2023

<p>Supplementary Figure 3 (a) CNE2 were transiently transfected with control mimic (Cel- 39), BART2-5p mimic, BART2-5p mimic + pcDNA3.1Vec, BART2-5p mimic + RND3 or BART2-5p and Y-27632 treatment. For control cells and BART2-5p transfected cells, F-Actin staining was performed to visualize stress fiber formation at 6 h after transfection. For cells transfected by BART2-5p and pcDNA3.1Vec or RND3 expressing vector, vector or RND3 was transfected 24 h earlier than transfection of BART2-5p to allow gene expression in cells. Pictures were taken at 6 h after transfection of BART2-5p. 1Î¼M ROCK inhibitor Y-27632 was added to culture medium for a 15min incubation at 6 h after transfection of BART2-5p before fixation. (b) CNE2 cells with BART2 expression or RND3 knock down were lysed for Western Blot. Either BART2 overexpression or RND3 knock down led to increased phosphorylated MLC2 and MYPT1. (c) Western Blot shows induced activation of ROCK signaling by BART2 in CNE2 cells could be abolished by reinstatement of RND3 or Y-27632 treatment. (d)	Images of Western Blot show alteration of key components in ROCK signaling pathway in siBART2-5p transfected or RND3 overexpressed HONE-1 EBV (+/-) cells.</p>
Figure S2 from Epstein–Barr Virus miRNA BART2-5p Promotes Metastasis of Nasopharyngeal Carcinoma by Suppressing RND3
Authors: Jiang Chen , Li Lei , Xiang Yan-Qun , Lung Maria Li , Zeng Tingting , Lu Jiabin , Tsao Sai Wah , Zeng Mu-Sheng , Yun Jing-Ping , Kwong Dora L.W. , Guan Xin-Yuan 
Published: April 2023

<p>Supplementary Figure 2 (a)	The four potential binding sites (position 931,1497,1522,1635) on the 3'UTR were predicted by the PITA analysis. Bases labeled blue were the mutated nucleotides of potential binding sites. (b)	Downregulated RND3 expression in BART2 expressing CNE2 cells was revealed by western blot. (c) RIP assay was performed with CNE2 cells with Vector or BART2 expression. The RT-PCR revealed that RND3 3'UTR was enriched on the RISC complex in BART2 expressing cells, but not in cells carrying vector. Input: cell lysate before immunoprecipitation. NC: precipitates pulled down by isotype control mouse IgG. Ago2: precipitates pulled down by anti-Ago2 antibody. Ago2 is core component of RISC. (d) Quality control of RIP assay. Western Blot confirmed that Ago2 was successfully and specifically pulled down by anti-Ago2 antibody. Light and heavy chains of primary antibody were also detected. (e) RND3 expression was inhibited in lung metastatic lesions formed by BART2 expressing cells. (f) Knock down efficiency of RND3 by shRNA was determined by western blot. (Left) Migration and invasion assays were performed to evaluate cell motility after knock down RND3. Migrated or invaded cells were counted and statistically compared in the bar chart. (Middle and Right) (g) Successful reconstitution of RND3 was confirmed by western blot. (Left) Cell migration and invasion was evaluated by transwell assay. Migrated or invaded cells were counted and statistically compared in the bar chart. (Middle and Right) (h) HONE-1 EBVï¼^+ï¼‰and HONE-1 cells were transfected with Control mimic/siRNA targeting BART2-5p or infected by lentivirus to establish stable cell lines with Vector/RND3 expression. Migration assays were performed 8h after transient transfection or once Blasticidin selection finished. Migrated cells were counted under microscope for following statistical analysis. (i) HONE-1 EBVï¼^+ï¼‰and HONE-1 cells were transfected with Control mimic/siRNA targeting BART2-5p or infected by lentivirus to establish stable cell lines with Vector/RND3 expression. Cell invasiveness was evaluated 8h after transient transfection or once Blasticidin selection finished. Invaded cells were counted under microscope for subsequent statistical analysis.</p>
Supplementary tables & figures from A miRNA Expression Signature in Breast Tumor Tissue Is Associated with Risk of Distant Metastasis
Authors: Rohan Thomas E. , Wang Tao , Weinmann Sheila , Wang Yihong , Lin Juan , Ginsberg Mindy , Loudig Olivier 
Published: April 2023

<p>Supplementary tables showing: (1)	Associations of clinical characteristics with risk of metastasis in Stage 1 and 2; (2)	Multivariate analysis of clinical variables (3)	Kegg pathway analysis (4)	AUCs of the miRNA score for breast cancer subtypes (5)	Summary of previous studies Supplementary figures showing (6)	Sample distribution in sequencing libraries (7)	Repeatability of qPCR</p>
Supplementary Data from Time-Dependent Effects of Oral Contraceptive Use on Breast, Ovarian, and Endometrial Cancers
Authors: Karlsson Torgny , Johansson Therese , Höglund Julia , Ek Weronica E. , Johansson Åsa 
Published: April 2023

<p>Online Data Supplements: â€¢	Online methods. â€¢	Supplementary Table S1. A selection of previous studies aiming to estimate the effect of oral contraceptive use on risk of breast cancer. â€¢	Supplementary Table S2. Identification of cancer cases in the UK biobank database. â€¢	Supplementary Table S3. Identification of covariates in the UK biobank database and their Odds ratio [OR] and 95% confidence interval [CI]) on odds of cancer. â€¢	Supplementary Table S4. Fixed (time-independent) associations of oral contraceptives, during and after use, as analysed by Cox regression modelling. â€¢	Supplementary Table S5. Fixed (time-independent) associations of oral contraceptives during and after use, as analysed by Cox regression modelling with age as primary time scale (sensitivity analyses). â€¢	Supplementary Table S6. Time-dependent associations of oral contraceptives during use, as analysed by Cox regression modelling. â€¢	Supplementary Table S7. Time-dependent hazard ratios of oral contraceptives after last use. â€¢	Supplementary Figure S1. Sensitivity analyses for the associations between oral contraceptive use and cancer in the Cox regression. â€¢	Supplementary Figure S2. Discrepancy between self-reported data and cancer register. â€¢	Supplementary Figure S3. Power calculations. â€¢	Supplementary Figure S4. Distributions for age at menopause. â€¢	Supplementary Figure S5. Sensitivity analyses for different follow-up years after oral contraceptive discontinuation.</p>
Supplementary Data from HDAC5 Loss Impairs RB Repression of Pro-Oncogenic Genes and Confers CDK4/6 Inhibitor Resistance in Cancer
Authors: Zhou Yingke , Jin Xin , Ma Jian , Ding Donglin , Huang Zhenlin , Sheng Haoyue , Yan Yuqian , Pan Yunqian , Wei Ting , Wang Liguo , Wu Heshui , Huang Haojie 
Published: April 2023

<p>The file includes: â€¢	Supplementary Materials and Methods â€¢	Supplementary References â€¢	Supplementary Table S1. Sequence of gene specific shRNAs â€¢	Supplementary Table S2. Sequence of primers for RT-qPCR and ChIP-qPCR â€¢	Supplementary Table S3. Information of antibodies â€¢	Supplementary Table S4. Information of chemicals â€¢	Supplementary Table S5. Information of cell lines and recombinant DNA â€¢	Supplementary Table S6. QC metrics of RNA-seq data â€¢	Supplementary Table S7. Basic characteristics of ChIP-seq data â€¢	Supplementary Table S8. Top 20 enriched pathways identified by GSEA analysis â€¢	Supplementary Fig. S1. Expression of HDAC family members in the TCGA PRAD database and the effect of HDAC4 and HDAC5 dual inhibitor on PCa cell sensitivity to Palbociclib â€¢	Supplementary Fig. S2. The effect of HDAC5 deficiency on breast cancer cell sensitivity to Palbociclib â€¢	Supplementary Fig. S3. CDK4/6 phosphorylation of RB on S249/T252 impedes RB-N interaction with HDAC5 â€¢	Supplementary Fig. S4. CDK2 phosphorylation of RB on T821 dampens RB-C interaction with HDAC5 â€¢	Supplementary Fig. S5. HDAC5 is essential for Palbociclib-induced deacetylation on H3K27 â€¢	Supplementary Fig. S6. RB-HDAC5 regulate H3K27-ac in an E2F1 dependent manner</p>
Figure S3 from An AIB1 Isoform Alters Enhancer Access and Enables Progression of Early-Stage Triple-Negative Breast Cancer
Authors: Sharif Ghada M. , Campbell Moray J. , Nasir Apsra , Sengupta Surojeet , Graham Garrett T. , Kushner Max H. , Kietzman William B. , Schmidt Marcel O. , Pearson Gray W. , Loudig Olivier , Fineberg Susan , Wellstein Anton , Riegel Anna T. 
Published: April 2023

<p>A)	Representative images of hematoxylin and eosin (H&E) staining of MCFDCIS, MCFDCIS-Î"4 and mixed (4:1 respectively) tumors that were injected in the mammary fat pad of SCID/Beige mice after two or five weeks. MCFDCIS-Î"4 tumors were not collected at two weeks due to small tumor size. Scale bar=50Î¼m. B)	Growth of subcutaneous tumors of DCIS or DCIS-Î"4-hy in athymic nude mice. n=5. **p<0.01. H&E tumor histology at 3 weeks. Scale bar = 50Î¼m. C)	Quantification of P63 and Luciferase (unique to DCIS) co-localization in mixed tumors at 2 and 5 weeks. ROI= 2007x1500um. **p<0.01. D)	Representative images and quantification of p63 and CD44 IHC on tumors from S3B. **p<0.01, ***p<0.001. Scale bar = 50Î¼m. E)	Bioluminescence images showing lung metastases at 2-3 months. Signal is detected from parental DCIS that harbor the luciferase construct. DCIS-Î"4 cells are luciferase negative. F)	qPCR of genomic DNA from the lungs of mice harboring mix tumors. Primers are specific to either the unique region on AIB1 gene that was created by CRISPR editing (CRISPR) or human intronic DNA as a control (Intronic). Mouse intronic DNA was used for normalization. G)	Bioluminescence images and graph showing tumor recurrence rate after DCIS or Mix primary tumor removal when they reached an area of ~80mm2. Two million cells were injected in the MFP of SCID mice. H)	qPCR of human genomic DNA for either the unique region on AIB1 gene that was created by CRISPR editing (CRISPR) or human intronic DNA as a control (Intronic). MCFDCIS and MCFDCIS-Î"4 cell lines were used as reference points for MCFDCIS and Mix tumors (MCFDCIS and MCFDCIS-Î"4).</p>
Supplementary Figures 1 - 2 from Identification of Circulating MicroRNA Signatures for Breast Cancer Detection
Authors: Chan Maurice , Liaw Chiew Suan , Ji Shen Mo , Tan Hwee Huang , Wong Chow Yin , Thike Aye Aye , Tan Puay Hoon , Ho Gay Hui , Lee Ann Siew-Gek 
Published: April 2023

<p>PDF file - 189K, Supplementary Fig. S1:	Three components principal component analysis of (a) tissue and (b) serum samples. Breast cancer tumour tissues (a) and serum from breast cancer patients (b) are indicated in red. Adjacent normal tissues (a) and serum from healthy individuals (b) are indicated in blue. Supplementary Fig. S2:	Correlation plots from inter-platform comparison studies. (a) same breast cancer tumour sample extracted by mirVana and miRNeasy; (b) same breast cancer tumour sample ran on microarray and RT-PCR panels.</p>