bluuebunny/crossref_metadata_2025_split huggingface dataset:
Cow’s milk allergy skin tests: fresh milk, commercial extracts, or both?
Authors: Lachover-Roth Idit , Giorno Nadav , Hornik-Lurie Tzipi , Cohen-Engler Anat , Rosman Yossi , Meir-Shafrir Keren , Confino-Cohen Ronit 
Published: January 2023

Abstract
Background
The diagnosis of food allergy is based on a history of immediate allergic reaction following food ingestion, and skin prick test (SPT) demonstrating sensitization with commercial extracts (CE) or fresh food (FF). For most food allergens, the SPT with FF is considered more accurate and predictive. Regarding cow’s milk, the results are inconclusive. This retrospective study aimed to evaluate the accuracy of SPT with fresh milk compared to CE (cow’s milk and casein) for evaluation of cow’s milk allergy (CMA).

Methods
This study summarized the medical records of children, diagnosed with CMA. The data include demographics, skin tests and oral food challenge results, as well as atopic comorbidities.

Results
Records of 698 patients with the diagnosis of CMA were reviewed, 388 fulfilled the inclusion criteria. Overall, 134 patients (34.54%) had an additional atopic disease. The SPT wheal size with fresh milk was significantly larger than with CE (cow's milk and casein) at first evaluation or before oral food challenge (OFC). Combination of SPT results (CE and FF) gave the maximal odds ratio for reaction during OFC and SPT with fresh milk alone gave the minimal OR (34.18 and 4.74, respectively).

Conclusions
SPT with CE for CMA evaluation is more reliable than SPT performed with fresh milk. In patients suspected of having IgE-mediated CMA, before deciding on performing OFC, it is advised to perform SPT with at least two different extracts, and always include casein. Fresh milk can serve as a backup if commercial extracts are not available. In cases that the SPT with fresh milk is 3 mm or less, there is 93.3% chance that the OFC will pass without reaction.
Trial registration This study protocol was reviewed and approved by the Ethics Committee of Meir Medical Center, IRB Number 0083-18 MMC.
MRI anatomy of the rectum: key concepts important for rectal cancer staging and treatment planning
Authors: Bogveradze Nino , Snaebjornsson Petur , Grotenhuis Brechtje A. , van Triest Baukelien , Lahaye Max J. , Maas Monique , Beets Geerard L. , Beets-Tan Regina G. H. , Lambregts Doenja M. J. 
Published: January 2023

AbstractA good understanding of the MRI anatomy of the rectum and its surroundings is pivotal to ensure high-quality diagnostic evaluation and reporting of rectal cancer. With this pictorial review, we aim to provide an image-based overview of key anatomical concepts essential for treatment planning, response evaluation and post-operative assessment. These concepts include the cross-sectional anatomy of the rectal wall in relation to T-staging; differences in staging and treatment between anal and rectal cancer; landmarks used to define the upper and lower boundaries of the rectum; the anatomy of the pelvic floor and anal canal, the mesorectal fascia, peritoneum and peritoneal reflection; and guides to help discern different pelvic lymph node stations on MRI to properly stage regional and non-regional rectal lymph node metastases. Finally, this review will highlight key aspects of post-treatment anatomy, including the assessment of radiation-induced changes and the evaluation of the post-operative pelvis after different surgical resection and reconstruction techniques.
Study results from journals with a higher impact factor are closer to “truth”: a meta-epidemiological study
Authors: Heidenreich Andreas , Eisemann Nora , Katalinic Alexander , Hübner Joachim 
Published: January 2023

Abstract
Background
Scientists, physicians, and the general public legitimately expect scholarly publications to give true answers to study questions raised. We investigated whether findings from studies published in journals with higher Journal Impact Factors (JIFs) are closer to truth than findings from studies in less-cited journals via a meta-epidemiological approach.

Methods
We screened intervention reviews from the Cochrane Database of Systematic Reviews (CDSR) and sought well-appraised meta-analyses. We used the individual RCT study estimates’ relative deviation from the pooled effect estimate as a proxy for the deviation of the study results from the truth. The effect of the JIF on the relative deviation was estimated with linear regression and with local polynomial regression, both with adjustment for the relative size of studies. Several sensitivity analyses for various sub-group analyses and for alternative impact metrics were conducted.

Results
In 2459 results from 446 meta-analyses, results with a higher JIF were on average closer to “truth” than the results with a lower JIF. The relative deviation decreased on average by −0.023 per JIF (95% CI −0.32 to −0.21). A decrease was consistently found in all sensitivity analyses.

Conclusions
Our results indicate that study results published in higher-impact journals are on average closer to truth. However, the JIF is only one weak and impractical indicator among many that determine a studies’ accuracy.
An improvement of a nonuniform bound for unbounded exchangeable pairs
Authors: Sumritnorrapong Patcharee , Suntornchost Jiraphan 
Published: January 2023

AbstractIn this paper, we obtain a nonuniform Berry–Esseen bound for a normal approximation via the Stein method and the exchangeable-pair coupling technique where the boundedness condition of the difference between the exchangeable pair is not required. As applications of the result, we obtain nonuniform bounds for the normal approximations in two well-known applications that are the independence test and the quadratic form. Our results suggest that the obtained bounds for the two applications are sharper than other existing bounds.
Essential medicines for mental disorders: comparison of 121 national lists with WHO recommendations
Authors: Todesco Beatrice , Ostuzzi Giovanni , Gastaldon Chiara , Papola Davide , Barbui Corrado 
Published: January 2023

Abstract
Background
To compare the medicines for mental disorders included in national essential medicines lists with the World Health Organization (WHO) essential medicines list and assess the extent to which economic status and WHO Region account for the differences.

Methods
We searched WHO repository and government sites for national essential medicines lists and we abstracted medicines for mental disorders. We calculated the proportion of WHO essential medicines included, the total number of differences (counting both additions and deletions) between national and WHO model list and the proportion of lists including one second-generation oral antipsychotic plus one new-generation antidepressant. Non-parametric statistics was used to investigate whether these indicators were dependent on economic status and WHO Region.

Results
Amongst the 121 identified national lists, the total number of medicines for mental disorders ranged from 2 to 63 (median: 18; IQR: 14 to 25). The median proportion of WHO essential medicines for mental disorders included was 86% (IQR: 71–93%), with 16 countries (13%, 95% CI 7.75–20.5%) including all WHO essential medicines, while the median number of differences with the WHO EML was 11 (IQR: 7 to 15). Country economic level was positively associated with both the proportion of WHO essential medicines included (Spearman's rho = 0.417, p < 0.001) and the number of differences (Spearman's rho = 0.345, p < 0.001), implying that countries with higher income level included more WHO essential medicines, but also more additional medicines. Significant differences were observed in relation to WHO Region, with the African and Western Pacific Region showing the lowest proportions of WHO essential medicines, and the European Region showing the highest median number of differences. Overall, 88 national lists (73%, 95% CI 63–80%) included at least one second-generation oral antipsychotic and new-generation antidepressant, with differences by income level and WHO Region.

Conclusions
The degree of alignment of national lists with the WHO model list is substantial, but there are considerable differences in relation to economic status and WHO Region. These findings may help decision-makers to identify opportunities to improve national lists, aiming to increase access to essential medicines for mental disorders.
Association between central venous pressure measurement and outcomes in critically ill patients with severe coma
Authors: Tong Xin , Feng Xin , Duan Chuanzhi , Liu Aihua 
Published: January 2023

Abstract
Background
The use of central venous pressure (CVP) measurements among (intensive care unit) ICU patients with severe coma has been questioned. This study aimed to investigate the application value of CVP in this population.

Methods
Data stored in the ICU Collaborative Research Database (eICU-CRD) and Medical Information Mart for Intensive Care III (MIMIC-III) database were reviewed. Critically ill patients with a Glasgow Coma Scale (GCS) score of 3–8 were included. The primary outcome was the in-hospital mortality rate. The statistical approaches used included multivariable Cox regression, propensity score matching (PSM), inverse probability treatment weighting (IPTW), stabilized IPTW, and restricted cubic splines (RCS) to ensure the robustness of our findings.

Results
In total, 7386 patients were included in the study. Early CVP measurement was independently associated with in-hospital mortality [hazard ratio, 0.63; p < 0.001] in patients with severe-to-moderate coma. This result was robust in the PSM, sIPTW, and IPTW cohorts. For all patients with CVP measurements, the RCS curves showed that the risk of in-hospital mortality increased as the initial CVP time was delayed. In addition, early CVP measurement was significantly associated with lower ICU mortality, 28-day mortality, and 365-day mortality and a significantly higher number of ventilator-free days.

Conclusion
Early CVP measurement could improve clinical outcomes in critically ill patients with severe coma
Dietary Epimedium extract supplementation improves intestinal functions and alters gut microbiota in broilers
Authors: Zhang Jiaqi , Yu Haitao , Zhang Huiyan , Zhao Qingyu , Si Wei , Qin Yuchang , Zhang Junmin 
Published: January 2023

Abstract
Background
Growth-promoting antibiotics have been banned by law in the livestock and poultry breeding industry in many countries. Various alternatives to antibiotics have been investigated for using in livestock. Epimedium (EM) is an herb rich in flavonoids that has many beneficial effects on animals. Therefore, this study was planned to explore the potential of EM as a new alternative antibiotic product in animal feed.

Methods
A total of 720 1-day-old male broilers (Arbor Acres Plus) were randomly divided into six groups and fed basal diet (normal control; NC), basal diet supplemented with antibiotic (75 mg/kg chlortetracycline; CTC), and basal diet supplemented with 100, 200, 400 or 800 mg/kg EM extract for 6 weeks (EM100, EM200, EM400 and EM800 groups). The growth performance at weeks 3 and 6 was measured. Serum, intestinal tissue and feces were collected to assay for antioxidant indexes, intestinal permeability, lactic acid and short-chain fatty acids (SCFAs) profiles, microbial composition, and expression of intestinal barrier genes.

Results
The average daily feed intake in CTC group at 1–21 d was significantly higher than that in the NC group, and had no statistical difference with EM groups. Compared with NC group, average daily gain in CTC and EM200 groups increased significantly at 1–21 and 1–42 d. Compared with NC group, EM200 and EM400 groups had significantly decreased levels of lipopolysaccharide and D-lactic acid in serum throughout the study. The concentrations of lactic acid, acetic acid, propionic acid, butyric acid and SCFAs in feces of birds fed 200 mg/kg EM diet were significantly higher than those fed chlortetracycline. The dietary supplementation of chlortetracycline and 200 mg/kg EM significantly increased ileal expression of SOD1, Claudin-1 and ZO-1 genes. Dietary supplemented with 200 mg/kg EM increased the relative abundances of g_NK4A214_group and Lactobacillus in the jejunal, while the relative abundances of Microbacterium, Kitasatospora, Bacteroides in the jejunal and Gallibacterium in the ileum decreased.

Conclusion
Supplementation with 200 mg/kg EM extract improved the composition of intestinal microbiota by regulating the core bacterial genus Lactobacillus, and increased the concentration of beneficial metabolites lactic acid and SCFAs in the flora, thereby improving the antioxidant capacity and intestinal permeability, enhancing the function of tight junction proteins. These beneficial effects improved the growth performance of broilers.

Graphical Abstract
Dlk1 maintains adult mice long-term HSCs by activating Notch signaling to restrict mitochondrial metabolism
Authors: Huang Deyu , Han Yingli , Tang Tian , Yang Lin , Jiang Penglei , Qian Wenchang , Zhang Zhaoru , Qian Xinyue , Zeng Xin , Qian Pengxu 
Published: January 2023

Abstract
Background
Adult hematopoietic stem cells (HSCs) homeostasis is critically important in maintaining lifelong hematopoiesis. However, how adult HSCs orchestrate its homeostasis remains not fully understood. Imprinted gene Dlk1 has been shown to play critical role in mouse embryonic hematopoiesis and in regulation of stem cells, but its physiological roles in adult HSCs are unknown.

Methods
We performed gene expression analysis of Dlk1, and constructed conditional Dlk1 knockout (KO) mice by crossing Mx1 cre mice with Dlkflox/flox mice. Western blot and quantitative PCR were used to detect Dlk1 KO efficiency. Flow cytometry was performed to investigate the effects of Dlk1 KO on HSCs, progenitors and linage cells in primary mice. Competitive HSCs transplantation and secondary transplantation was used to examine the effects of Dlk1 KO on long-term hematopoietic repopulation potential of HSCs. RNA-Seq and cell metabolism assays was used to determine the underlying mechanisms.

Results
Dlk1 was highly expressed in adult mice long-term HSCs (LT-HSCs) relative to progenitors and mature lineage cells. Dlk1 KO in adult mice HSCs drove HSCs enter active cell cycle, and expanded phenotypical LT-HSCs, but undermined its long-term hematopoietic repopulation potential. Dlk1 KO resulted in an increase in HSCs’ metabolic activity, including glucose uptake, ribosomal translation, mitochondrial metabolism and ROS production, which impaired HSCs function. Further, Dlk1 KO in adult mice HSCs attenuated Notch signaling, and re-activation of Notch signaling under Dlk1 KO decreased the mitochondrial activity and ROS production, and rescued the changes in frequency and absolute number of HSCs. Scavenging ROS by antioxidant N-acetylcysteine could inhibit mitochondrial metabolic activity, and rescue the changes in HSCs caused by Dlk1 KO.

Conclusion
Our study showed that Dlk1 played an essential role in maintaining HSC homeostasis, which is realized by governing cell cycle and restricting mitochondrial metabolic activity.
Minimal expression of dysferlin prevents development of dysferlinopathy in dysferlin exon 40a knockout mice
Authors: Yasa Joe , Reed Claudia E. , Bournazos Adam M. , Evesson Frances J. , Pang Ignatius , Graham Mark E. , Wark Jesse R. , Nijagal Brunda , Kwan Kim H. , Kwiatkowski Thomas , Jung Rachel , Weisleder Noah , Cooper Sandra T. , Lemckert Frances A. 
Published: January 2023

AbstractDysferlin is a Ca2+-activated lipid binding protein implicated in muscle membrane repair. Recessive variants in DYSF result in dysferlinopathy, a progressive muscular dystrophy. We showed previously that calpain cleavage within a motif encoded by alternatively spliced exon 40a releases a 72 kDa C-terminal minidysferlin recruited to injured sarcolemma. Herein we use CRISPR/Cas9 gene editing to knock out murine Dysf exon 40a, to specifically assess its role in membrane repair and development of dysferlinopathy. We created three Dysf exon 40a knockout (40aKO) mouse lines that each express different levels of dysferlin protein ranging from ~ 90%, ~ 50% and ~ 10–20% levels of wild-type. Histopathological analysis of skeletal muscles from all 12-month-old 40aKO lines showed virtual absence of dystrophic features and normal membrane repair capacity for all three 40aKO lines, as compared with dysferlin-null BLAJ mice. Further, lipidomic and proteomic analyses on 18wk old quadriceps show all three 40aKO lines are spared the profound lipidomic/proteomic imbalance that characterises dysferlin-deficient BLAJ muscles. Collective results indicate that membrane repair does not depend upon calpain cleavage within exon 40a and that ~ 10–20% of WT dysferlin protein expression is sufficient to maintain the muscle lipidome, proteome and membrane repair capacity to crucially prevent development of dysferlinopathy.
Bayesian historical borrowing with longitudinal large-scale assessments
Authors: Kaplan David , Chen Jianshen , Lyu Weicong , Yavuz Sinan 
Published: January 2023

AbstractThe purpose of this paper is to extend and evaluate methods of Bayesian historical borrowing applied to longitudinal data with a focus on parameter recovery and predictive performance. Bayesian historical borrowing allows researchers to utilize information from previous data sources and to adjust the extent of borrowing based on the similarity of current data to historical data. We examine the utility of three static historical borrowing methods including complete pooling, Bayesian synthesis with aggregated data-dependent priors, traditional power priors, and two dynamic borrowing methods including Bayesian dynamic borrowing and commensurate priors. Using data from two administrations of the United States Early Childhood Longitudinal Study, we evaluate these methods in terms of in-sample simulation statistics, as well as pseudo out-of-sample measures of predictive performance. A case study examining growth in reading competency over time revealed that for one historical cycle, most methods of historical borrowing perform similarly with respect to predictive performance and parameter recovery. Pooling and power priors performed relatively poorly across the conditions in this study, particularly when the current data and the historical data were heterogeneous. Results from a comprehensive simulation study revealed that the advantages of different historical borrowing methods vary across different evaluation criteria. Overall, Bayesian dynamic borrowing and commensurate priors are no worse, and in some cases better, than other methods in terms of parameter recovery and predictive performance, and considering a previous paper by Kaplan et al. (Psychometrika, 10.1007/s11336-022-09869-3, 2022) found clear benefits of Bayesian dynamic borrowing over other methods of historical borrowing in the multilevel context using data from the Program for International Student Assessment (PISA) with five historical cycles, this paper argues that Bayesian dynamic borrowing or commensurate priors is a prudent choice for borrowing information from previous cycles of longitudinal data.
Development of a certified reference material for the analysis of vitamins in multivitamin tablets
Authors: Lee Joonhee , Kim Byungjoo , Sim Hee-Jung , Seo Dongwon , Kwak Byung-Man , Won Jongeun , Lee Sunyoung , Baek Song-Yee , Han Jeesoo 
Published: January 2023

AbstractMultivitamin tablet certified reference material (CRM, 108-10-019) was developed for the analysis of seven water-soluble vitamins, including thiamine, riboflavin, nicotinamide, pantothenic acid, pyridoxine, biotin, and folic acid. The CRM was prepared in powder form by grinding multivitamin tablets and then mixing, sieving, and bottling the powder. For the certification of each water-soluble vitamin, the isotope dilution mass spectrometry based on the liquid chromatography was applied. The methods for each analyte were validated by confirming the repeatability and reproducibility and by comparing with other CRMs. The property values and uncertainties for the vitamins were determined with 10 units from sample stored at − 20 °C. The homogeneity of each certified component was also examined in the range of 0.48–2.2%. All certified values for the seven water-soluble vitamins were stable for 3 or 6 years after the initial certification under storage conditions at − 20 °C. For fat-soluble vitamins, including retinol, α-tocopherol, cholecalciferol, and phylloquinone, two expert laboratories participated in analyses based on official methods, and the mean values of the reported results were assigned as reference values. The multivitamin tablet CRM (108-10-019) will be useful for validating analytical methods and for ensuring the quality of results for vitamin analysis in multivitamin tablets or similar products.
Uplift rate of Kitadaito Jima Island on the lithospheric forebulge of the Philippine Sea Plate
Authors: Iryu Yasufumi , Takayanagi Hideko , Ishikawa Tsuyoshi , Ishigaki Akimasa , Asanuma Takuji , Teruya Rin , Budd David A. 
Published: January 2023

AbstractEstimates of uplift rates for lithospheric forebulges are needed to understand exact plate motions at plate convergence zones and to delineate the fate of coral reefs atop the forebulges. A carbonate island on a lithospheric forebulge can provide excellent materials for estimating uplift rate because carbonate sediments can be dated and their paleo-water depth determined. We estimated the uplift rate of Kitadaito Jima Island, a carbonate island on the lithospheric forebulge of the Philippine Sea Plate that is subducting beneath the Eurasian Plate. Marine skeletal sands containing pebble-sized bioclasts and filling the concavity of an intertidal erosional notch at an elevation of ~ 71 m were found near the top of this island. Strontium isotope ages ranging from 1.78 to 2.01 Ma with an average of 1.89 Ma (standard deviation = 0.07 Ma) were obtained from these deposits. As global sea level at 1.89 Ma was ~ 21 m lower than the present, the mean uplift rate was estimated at ~ 49 m/million years (Myr) with a compounded uncertainty of ± 2.6 m/Myr. This rate is comparable to, or up to ~ 140 m/Myr less than, rates reported from other Indo-Pacific carbonate islands in similar tectonic settings. This study illustrates how contemporary Sr isotope age models and careful considerations of limestone sample depositional depths can yield more accurate and precise uplift rates of modern forearc bulges than possible in many older studies.