bluuebunny/crossref_metadata_2025_split huggingface dataset:
Knockdown of klotho Leads to Cell Movement Impairment during Zebrafish Gastrulation
Authors: Pan Heng-Chih , Lo Kang-Chieh , Wang Yun-Hsin , Huang Han-Ting , Cheng Shu-Chun , Sun Chiao-Yin , Chen Yau-Hung 
Published: August 2023

(1) Background: Klotho is a multifunctional protein, but its biophysiological roles during fish early development are unclear. (2) Methods: We carried out anti-sense Klotho morpholino injection and whole-mount in situ hybridization experiments in this study. (3) Results: Our results showed that in the Klotho MO1-injected group, embryos displayed longitudinal shapes and swelling yolks compared to those of the un-injected groups. Their length/width ratio by 9 hpf for the uninfected group is 1 ± 0.1; but for 0.1 mM-, 0.2 mM-, and 0.5 mM-MO-injected are 1.1 ± 0.1, 1.2 ± 0.1, and 1.3 ± 0.1, respectively, suggesting that the klotho knockdown-induced length/width ratio changes are in a dose-dependent manner. Whole-mount in situ hybridization showed that several cell migration-related gene expressions, including goosecoid, floating head, bone morphogenetic protein 4, and even-skipped-liked 1 were affected in the Klotho MO-injected embryos. (4) Conclusions: We suggest that knockdown of klotho leads to cell movement impairment during zebrafish gastrulation.
Molecular Identification and Acid Stress Response of an Acidithiobacillus thiooxidans Strain Isolated from Rio Tinto (Spain)
Authors: Ibáñez Ana , Barreiro Carlos , Diez-Galán Alba , Cobos Rebeca , Calvo-Peña Carla , Coque Juan José R. 
Published: August 2023

Acidithiobacillus thiooxidans is of paramount importance in the development of biomining technologies. Being widely recognized as an extreme acidophile, extensive research has been dedicated to understanding its significant role in the extraction of several ores in recent years. However, there still exist significant molecular uncertainties surrounding this species. This study focuses on developing a taxonomic assignment method based on the sequencing of the 16S-5S rRNA cluster, along with a qPCR-based technology enabling precise growth determination. Additionally, an approach to understanding its response to acid stress is explored through RT-PCR and MALDI-TOF analysis. Our findings indicate that when subjected to pH levels below 1, the cell inhibits central (carbon fixation and metabolism) and energy (sulfur metabolism) metabolism, as well as chaperone synthesis, suggesting a potential cellular collapse. Nevertheless, the secretion of ammonia is enhanced to raise the environmental pH, while fatty acid synthesis is upregulated to reinforce the cell membrane.
Simulasi Tegangan (Stress) Pada Komponen Rangka Mesin Uji Tarik Sealent Menggunakan Solidworks
Authors: Prabowo Dian , Satria Jati Unggul , Ulikaryani Ulikaryani , Hardini Probo 
Published: August 2023

A series of processes are carried out in order to obtain the desired product result. One of the processes carried out is testing the strength of polymers by means of tensile testing in this case testing of polymer-based sealants. The method used is the method of simulating frame loading on the tensile testing machine that has been made. The software used to assist the simulation process is SolidWorks. The simulation is carried out in static mode or a fixed loading (no movement or vibration). A stress Analysis Simulation is carried out to get the result of static loading in the form of σ (stress). Loading simulations on the frame of the tensile testing machine for the tensile testing of the sealant are carried out on the components of the frame with different loading variations. Frame components with upward loading are given an average load of 38.5 MPa. Meanwhile, the frame components with downward loading are given an average load of 6.169 MPa. The largest average stress obtained from each component is 0.326 N/mm2. this value does not exceed the yield strength of 235 N/mm2.
CYTOTOXIC ACTIVITY OF BASIL SEEDS( Ocimum baslicum L) EXTRACTS ON SOME BREAST CANCER CELL LINES ( IN VITRO)
Authors: Ayeda M.M  , J. M. Awda  
Published: August 2023

In this study, four types of basil seed extracts were used, being cold aqueous extract, hot aqueous extract, petroleum ether extract, and methanolic extract.Nine concentrations (10000, 5000, 2500, 1250, 625, 312.56, 156.25, 78.125, 39.0, 0.0) µg/ml were used to study the extracts cytotoxicity against two breast cancer cell lines MCF7 and AMJ13 beside REF cell line as normal cells. The methanol extract showed the highest inhibition rate of 64.4% on MCF7 cell line at concentration of 1250 µg/ml and 42.4% on AMJ13 cell line at a concentration of 39.0 µg/ml with significant difference as compared to control sample during the 72hr exposure period. While the petroleum ether extract showed a lower inhibition rate of 16.02% at a concentration of 78.1 µg/ml on MCF7 cell line and 35.3 % at a concentration of 312.5 µg /ml on AMJ13 cells during a 72hr exposure period, compared with the two aqueous extracts which showed a slight effect compared to the control. The cold aqueous extract showed the slight effect of 24.4% at a concentration of 5000 µg/ml on AMJ13 cell line and no toxicity was shown on MCF7 cells. All extracts showed no effect on REF normal cells. In all effective concentrations the basil seed extracts caused a damage to the DNA that cannot be repaired, and thus the cells are heading to programmed death. This effect on the genetic material was studied using the comet assay and mitotic index.
ANALISIS PENDAPATAN PETANI SAWIT DARI ALIH FUNGSI LAHAN SAWIT DI DESA PONDOK BARU KECAMATAN SELAGAN RAYA KABUPATEN MUKOMUKO
Authors: Gusriati Gusriati , Sumarno Wawan , Sudarso Jos 
Published: August 2023

Tujuan penelitian ini adalah untuk mengetahui alasan petani melakukan alih fungsi lahan karet menjadi lahan sawit dan mempelajari pendapatan petani sawit dari alih fungsi lahan karet tersebut di Desa Pondok Baru Kecamatan Selagan Raya Kabupaten Mukomuko. Penelitian ini dilaksanakan pada bulan April-Mei 2022. Metode pelaksanaan penelitian adalah metode survey. Jumlah sampel sebanyak 85 petani dari 170 orang populasi. Analisis data menggunakan analisis deskriptif kuantitatif. Hasil penelitian menunjukkan alasan petani karet beralih ke sawit ada 4 faktor, yaitu : (a) Faktor modal sebanyak 2 orang (2,35%). (b) Faktor pendapatan sebanyak 38 orang (44,70%). (c) Faktor harga sebanyak 44 orang(51,76%). (d) dan Faktor produksi sebanyak 1 orang (1,17%). Pendapatan petani sawit dari alih fungsi lahan karet di Desa Pondok Baru Kecamatan Selagan Raya Kabupaten Mukomuko adalah sebesar Rp3.938.035/Ha/Bln.
DINAMIKA EKONOMI PETANI KARET
Authors: Sasmi Meli , Agustar Asdi , Syarfi Ira Wahyuni , Hasnah Hasnah 
Published: August 2023

Karet merupakan salah satu komoditi perkebunan yang berperan penting dalam membangun ekonomi petani terutama di pedesaan. Karet diperdagangkan secara internasional untuk kebutuhan bahan baku industri. Disebagian wilayah Indonesia komoditi karet dibudidayakan oleh perusahaan perkebunan (Estate) dan perkebunan rakyat, namun kondisi ekonomi petani karet dari waktu kewaktu mengalami penurunan. Berbagai faktor penyebabnya adalah: Rendahnya produksi karet, lahan berskala kecil, produkstivitas tenaga kerja rendah, panen tergantung kepada alam dan hanya bisa dipanen jika tidak hujan, belum ada teknologi untuk mengatasi kondisi alam serta teknologi yang mampu menggantikan tenaga kerja panen, selain itu pemasaran hasil karet masih dikuasai oleh pedagang pengumpul di desa. Harga ditingkat petani karet sangat cepat turun jika harga dipasaran internasioanal turun, sebaliknya harga akan lambat meningkat jika harga meningkat. Kondisi yang demikian menyebabkan rendahnya kemampuan ekonomi petani. Sering dikonotasikan bahwa wilayah yang perekonomian masyarakatnya berbasis komoditi karet, adalah wilayah lumbung kemiskinan. Mengatasi permasalahan tersebut maka perlu kebijakan pengembangkan lembaga ekonomi petani karet untuk menjaga stabilitas harga karet dan meningkatkan bargaining position petani perlu adanya regulasi mendukung kebijakan berupa mandatori dalam pengembangan kelembagaan petani dalam memperbaiki tataniaga karet sertaperlu adanya pengembangan Badan Usaha milik petani yang bergerak pada kegiatan hilirisasi karet ditingkat petani.
Patient Safety in Undergraduate Nursing: Student Perspective
Authors: Fernanda Accioly de Lima Santos  , Lisboa de Vasconcelos Maria Viviane , Maria de Lourdes Fonseca Vieira  , Ana Clara Valente de Lima Melo  , Inara Lourenço Leitão  
Published: August 2023

Introduction: "Patient safety" needs to be addressed and discussed in a serious and responsible manner, where it is indispensable to equip future professionals to prevent adverse events and develop a culture of patient safety. Thus, there is a need to assess patient safety in undergraduate nursing, in the context of classroom teaching and curriculum internships. Objective: This study proposes to analyze students’ perception of patient safety at a university in Northeastern Brazil. Methodology: This is a unique instrumental exploratory case study using a qualitative approach. Participants were 12 students selected by intentional sampling. Data collection occurred via an online focus group. Data were processed in NVivo software, version 12.0 for qualitative research. Results: The data illustrated the students’ perception of patient safety in three categories: classroom teaching; curriculum internships; and attributes for the management of future nurses. Although the university does not offer a specific patient safety discipline, the issue is addressed from the start of the course, by all teachers in a satisfactory manner. Final comments: The case study found that training health professionals based on patient safety measures is one of the largest investments that universities can make, sharpening critical thinking in order to improve care and reduce hospital costs, and above all, preserve life.
FILSAFAT ALIRAN PROGRESIVISME DAN KAITANNYA UNTUK ANAK BERKEBUTUHAN KHUSUS DALAM SETTING PENDIDIKAN INKLUSI
Authors: Badiah Lutfi isni , Siti Masitoh  , Mochamad Nursalim  
Published: August 2023

Artikel ini berisi tentang pandangan aliran filsafat progresivisme dan kaitannya untuk anak berkebutuhan khusus (ABK) dalam setting pendidikan inklusi. Anak berkebutuhan khusus adalah anak yang mengalami keterbatasan atau hambatan, baik dari segi fisik, mental-intelektual, sosial, maupun emosional, yang kemudian berdampak secara signifikan pada proses perkembangannya dibandingkan dengan anak normal lain seusianya. Negara Indonesia telah menjamin pendidikan untuk ABK melalui pendidikan inklusif agar mereka dapat memaksimalkan kemampuan belajarnya dan menggali potensi dirinya. Artikel ini menggunakan metode penelitian kepustakaan (library research). Teknik pengumpulan data dilakukan dengan menelaah buku, literatur, catatan dan jurnal yang berkaitan dengan pandangan aliran progresivisme terhadap anak berkebutuhan khusus dan pandangan mengenai pendidikan inklusi. Data yang telah terkumpul, kemudian dilakukan analisis data dengan menggunakan reduksi data, penyajian data, verifikasi, dan penarikan kesimpulan. Dalam kaitannya aliran filsafat progresivisme dengan pendidikan inklusif untuk anak berkebutuhan khusus tercermin pada lima komponen yakni tenaga pendidik, kurikulum, pengelolaan kelas, system evaluasi dan akesibilitas.
Kata kunci: progresivisme, pendidikan inklusi, anak berkebutuhan khusus
Pemberdayaan Masyarakat dalam Program Sanitasi Air Bersih melalui Program CSR PT Paiton Energy
Authors: Hayati Ashlihul , Wulandari Ratu Mutiara , Ghozali Ahmad , Saputra Meldy , Lubab Muhammad , Wahyuni Nina Ari 
Published: August 2023

<p><em>Desa Binor merupakan salah satu desa di Kabupaten Probolinggo yang mengalami masalah krisis air bersih dikarenakan letak daerah di pesisir pantai menyebabkan sumber mata air mengandung garam dan zat organik yang tinggi. Masalah tersebut dapat diatasi dengan bantuan berbagai pihak salah satunya melalui pelaksanaan program CSR ini bertujuan untuk mengetahui dampak CSR Program Sanitasi Air Bersih yang dijalankan oleh PT Paiton Energy di Desa Binor, Kecamatan Paiton, Kabupaten Probolinggo. Pelaksanaan Program Sanitasi Air Bersih mengedepankan prinsip pemberdayaan masyarakat dengan melibatkan partisipasi masyarakat mulai dari perencanaan, implementasi, monitoring, hingga evaluasi. Metode yang digunakan dalam pelaksanaan program ini adalah sosialisasi, pelatihan dan pendampingan program. Adapun kegiatan yang telah dijalankan meliputi instalasi air bersih dan sosialisasi, pemasangan panel surya, pelatihan pengelolaan sampah kertas dan plastik, pelaksanaan pengelolaan sampah plastik dan diaper. Hasil yang diperoleh melalui program ini yaitu total penerima manfaat program sebanyak 400 KK, 186 KK miskin terserap, penanaman 20.000 pohon, pemasukan BUMDes dari pengelolaan air bersih sebesar Rp.24.000.000/bulan dan terlaksananya sistem distribusi air bersih yang lebih teratur, merata, dan lebih baik dibandingkan sebelumnya. Implementasi sanitasi air bersih ini kemudian dapat dilaksanakan di wilayah lain untuk mengatasi tantangan air bersih dan pengelolaan lingkungan yang holistik.</em> </p><p><strong>Kata kunci<em>: </em></strong><strong><em>C</em></strong><strong><em>orporate Social Responsibility</em></strong><strong><em>,</em></strong><strong><em> Implementasi</em></strong><strong><em>,</em></strong><strong><em> Sanitasi Air Bersih</em></strong><em></em></p>
Sikap Profesional Dan Loyalitas Kerja Perawat Di Masa Pandemi Covid-19
Authors: Rachmania Diana , Sunaringtyas Widyasih 
Published: August 2023

ABSTRAK
Kondisi pandemi COVID-9 menjadi tantangan berat bagi perawat dimana perawat memiliki resiko tinggi tertular COVID-19. Perawat tetap bekerja menyelesaikan tugasnya dalam merawat pasien secara bertanggung jawab sebagai bentuk loyalitas kerja perawat yang tinggi. Di sisi lain, perawat dalam berbagai kondisi termasuk di masa pandemic Covid-19 juga memiliki kewajiban profesi untuk tetap merawat pasien. Penelitian ini bertujuan untuk mengetahui hubungan sikap profesional dengan loyalitas kerja perawat pada masa pandemi COVID-19. Desain penelitian korelasional dengan pendekatan cross sectional. Populasi sebanyak 48 perawat dan diperoleh sampel sebanyak 48 responden melalui teknik total sampling. Variabel bebas penelitian ini adalah sikap profesional dan variabel terikatnya adalah loyalitas kerja perawat. Alat ukurnya menggunakan kuesioner dari masing-masing variabel. Data dianalisis dengan uji Spearman's rho dengan α < 0,05. Hasil penelitian menunjukkan bahwa sebagian besar responden (69%) memiliki sikap profesional yang baik dan sebagian besar responden (77,1%) memiliki loyalitas kerja yang tinggi. Hasil uji Spearman rho menunjukkan p value 0,000 dengan r = 0,702. Ada hubungan antara sikap profesional dengan loyalitas kerja perawat pada masa pandemi COVID-19. Perawat diharapkan mampu melaksanakan profesionalisme dan memiliki loyalitas kerja yang baik dalam melaksanakan asuhan keperawatan kepada pasien sehingga dapat memberikan pelayanan yang berkualitas.

Kata kunci: profesionalisme, loyalitas kerja, perawat, pandemi COVID-19
Assist in GHG Abatement of Offshore Ships: Design and Economic Analysis of an Integrated Utilization Model of Hydrogen-Powered Ship and Offshore Wind Power
Authors: Liu Xinyu , Yang Guogang , Sun Baixun , Wang Hao , Li Yue , Wang Renjie 
Published: August 2023

As a hopeful solution to help the shipping sector achieve the greenhouse gas (GHG) abatement goal of the IMO by 2050, the application of hydrogen-powered ships has attracted more and more attention. To solve the problem of hydrogen supplement for offshore polymer electrolyte membrane fuel cell (PEMFC) ships, this paper presents a scenario design for the integrated application of coastal hydrogen-powered ships and offshore wind power, and analyzes its techno-economic feasibility. This model concept considers the problems in offshore hydrogen transportation and offshore wind power development together. It provides a feasible integrated scheme and operation mode for the combination of offshore wind power and hydrogen energy storage. A transformation scheme is also provided for the marine ranch ships, and these PEMFC-powered ships are combined with the hydrogen production of offshore wind farms. The analysis results show that the integrated utilization model is economically feasible and with a significant effect on decarbonization. It also shows great potential for further expansion to other coastal areas.
INVESTIGATION OF THE CONSEQUENCES OF LARGE FIRES IN CITIES AND RURAL AREAS OF THE RUSSIAN FEDERATION IN 2010–2021
Authors: Удавцова Е.Ю. , Бобринев Е.В. , Кондашов А.А. , Рюмина С.И. Рюмина С.И. 
Published: August 2023

Проведена сравнительная оценка уровней пожарной опасности объектов защиты разной категории при крупных пожарах в городах и сельской местности Российской Федерации в 2010–2021 годах. Проанализированы показатели «наибольшее количество крупных пожаров в жилом секторе» и «наибольшее количество крупных пожаров на предприятиях промышленности»; «наибольшая гибель людей при пожарах в жилом секторе» и аналогичный показатель для городов; а также количество погибших на объектах социально-культурной сферы в сельской местности и в городах. В соответствии с оценкой по показателю «соотношение доли спасенных при крупных пожарах людей от суммарного количества погибших и спасенных людей» приведены уровни пожарной опасности в жилом секторе, на предприятиях промышленности и объектах социально-культурной сферы в сельской местности, аналогичный показатель для городов. Осуществлено сравнение по показателю «доля травмированных при пожарах людей от общего количества погибших и травмированных людей» в жилом секторе и аналогичного показателя для городов.
There was carried out the comparative assessment of fire hazard levels of objects of protection of different categories in case of large fires in cities and rural areas of the Russian Federation in 2010-2021. There are analyzed the following indicators «the largest number of large fires in the residential sector» and «the largest number of large fires at industrial enterprises»; «the greatest loss of life in residential fires» and a similar indicator for cities. There are also analyzed the number of fatalities at social and cultural facilities in rural areas and in cities. In accordance with the assessment of the indicator «the ratio of the proportion of people rescued in large fires from the total number of deaths and rescued people» there are listed the levels of fire danger in the residential sector, as well as at industrial enterprises and objects of social and cultural sphere in rural areas. The similar indicator is given for cities. There is carried out the comparison of the indicator «the proportion of people injured in fires from the total number of dead and injured people» in the residential sector and the similar indicator for cities.
REVISION OF THE METHODOLOGY FOR DETERMINING FIRE RISK VALUES AT INDUSTRIAL FACILITIES
Authors: Фомин М.В. , Рукавишников М.М. , Хатунцев А.Ю. , Воронина Е.А. 
Published: August 2023

В статье освещены промежуточные результаты научно-исследовательской работы, проводимой специалистами ФГБУ ВНИИПО МЧС России по переработке «Методики определения расчетных величин пожарного риска на производственных объектах». Описана актуальность проводимой работы, а также рассмотрены предложения по внесению изменений в действующую методику.
This article highlights the intermediate results of the research work carried out by specialists of FGBU VNIIPO EMERCOM of Russia on revision of «Methodology for determining fire risk values at industrial facilities». The relevance of the work is described, as well as proposals for making changes to the current methodology are considered.
Phytoremediation potential and its methods- a review.
Authors: Hajipour Sahebe , Alinia-Ahandani Ebrahim , Behnaz Shirani-Bidabadi , Zahra Alizadeh-Tarpoei , Selamoglu Zeliha , Akram Muhammad 
Published: August 2023

With the increase in the world's population and the subsequent increase in housing density, we are facing the problem of water, soil, and air pollution. On the other hand, the use of physical environmental purification methods is very expensive and can even cause environmental pollution. Phytoremediation is a cheap and accessible method that uses the potential of plants to remove or destroy dangerous contaminants from water, soil, and air. One of the major problems in Phytoremediation is that the process is time-consuming. This can be overcome by adding compounds such as nanoparticles in order to increase the speed. Special care must be given to using the correct doses of nanoparticles because they pass through cells and biological membranes, which may cause contaminations. Therefore, the use of appropriate doses of nanoparticles will eventually accelerate the phytoremediation process. The purpose of this study is to assess the recent discoveries in the Phytoremediation process and its methods.
First Constraints on Dense Molecular Gas at z = 7.5149 from the Quasar Pōniuā‘ena
Authors: Feruglio Chiara , Maio Umberto , Tripodi Roberta , Winters Jan Martin , Zappacosta Luca , Bischetti Manuela , Civano Francesca , Carniani Stefano , D’Odorico Valentina , Fiore Fabrizio , Gallerani Simona , Ginolfi Michele , Maiolino Roberto , Piconcelli Enrico , Valiante Rosa , Zanchettin Maria Vittoria 
Published: August 2023

Abstract
We report the detection of CO(6–5) and CO(7–6) and their underlying continua from the host galaxy of quasar J100758.264+211529.207 (Pōniuā‘ena) at z = 7.5149, obtained with the NOrthern Extended Millimeter Array. Pōniuā‘ena belongs to the HYPerluminous quasars at the Epoch of ReionizatION sample of 18 z > 6 quasars selected to be powered by supermassive black holes, which experienced the fastest mass growth in the first cosmic gigayear. The one reported here is the highest-redshift measurement of the cold and dense molecular gas to date. The host galaxy is unresolved, and the line luminosity implies a molecular reservoir of M(H2) = (2.2 ± 0.2) × 1010
M
⊙, assuming a CO spectral line energy distribution typical of high-redshift quasars and a conversion factor α = 0.8

M

⊙

(
K

km

s

−
1

pc

2

)

−
1

. We model the cold dust spectral energy distribution to derive a dust mass of M
dust = (1.7 ± 0.6) × 108
M
⊙ and thus, a gas-to-dust ratio ∼130. Both the gas and dust mass are remarkably similar to the reservoirs found for luminous quasars at z ∼ 6–7. We use the CO detection to derive an estimate of the cosmic mass density of H2,

Ω

H

2

≃
1.31
×

10

−
5

. This value is in line with the general trend suggested by literature estimates at z < 7 and agrees fairly well with the latest theoretical expectations of nonequilibrium molecular-chemistry cosmological simulations of cold gas at early times.