bluuebunny/crossref_metadata_2025_split huggingface dataset:
Nano Iron Versus Bulk Iron Forms as Functional Feed Additives: Growth, Body Indices, Hematological Assay, Plasma Metabolites, Immune, Anti-oxidative Ability, and Intestinal Morphometric Measurements of Nile tilapia, Oreochromis niloticus
Authors: Mohammady Eman Y. , Elashry Mohamed A. , Ibrahim Mohamed S. , Elarian Mohamed , Salem Shimaa M. R. , El-Haroun Ehab R. , Hassaan Mohamed S. 
Published: June 2023

AbstractThe current study aimed to compare the utilization efficiency of iron (Fe) feed additives from either bulk or nano sources in Nile tilapia, Oreochromis niloticus diets on growth, haematological, immunity, anti-oxidative, and intestinal topography capacities. Five isonitrogenous and isoenergetic diets were performed; the basal diet served as a control with no Fe added, whereas the experimental diets were shaped by adding bulk-Fe2O3 and nano-Fe2O3 to the basal diet to preserve Fe levels at 0.2 and 0.4 mg kg−1, respectively. Results indicated that superior growth performance was recorded in fish-fed diets supplemented with 0.4 nano-Fe2O3 mg kg−1 diet. In addition, the highest (P ≤ 0.05) survival rate, absorption area of villous (AAV), mucosal to serosal amplification ratio (MSR), and villi parameters (height and width) were noticed in fish fed diet enrichment with either bulk or nano-Fe2O3 source. However, the superiority observed in nano-Fe2O3 fish groups. Also, the highest values of plasma albumin, total protein, high-density lipoprotein cholesterol (HDL-C), white blood cells (WBCs), and lymphocyte absolute count (LYM) (P ≤ 0.05) recorded in fish fed a diet supplemented with nano-Fe2O3 versus the basal diet. Moreover, the highest values of catalase (CAT), glutathione peroxidase (GPx), superoxide dismutase (SOD), and plasma lysozyme activity (P ≤ 0.05) were observed in fish fed 0.4 mg/kg−1 nano-Fe2O3, while the lowest value was recorded in fish fed the control diet. The best value of malondialdehyde activity (P ≤ 0.05) recorded in a fish-fed diet supplemented with 0.4 mg/kg−1 nano-Fe2O3. The current findings emphasize the importance of including Fe to improve fish growth, immunity, antioxidant capabilities, and intestinal structure, primarily with a nano-Fe source, which demonstrated a more effective function in satisfying Nile tilapia dietary Fe requirements and improving the aforementioned parameters.
Resolution of Cerebral Inflammation Following Subarachnoid Hemorrhage
Authors: Patsouris Victor , Blecharz-Lang Kinga G. , Nieminen-Kelhä Melina , Schneider Ulf C. , Vajkoczy Peter 
Published: June 2023

Abstract
Background
Aneurismal subarachnoid hemorrhage (SAH) is a type of hemorrhagic stroke that, despite improvement through therapeutic interventions, remains a devastating cerebrovascular disorder that has a high mortality rate and causes long-term disability. Cerebral inflammation after SAH is promoted through microglial accumulation and phagocytosis. Furthermore, proinflammatory cytokine release and neuronal cell death play key roles in the development of brain injury. The termination of these inflammation processes and restoration of tissue homeostasis are of utmost importance regarding the possible chronicity of cerebral inflammation and the improvement of the clinical outcome for affected patients post SAH. Thus, we evaluated the inflammatory resolution phase post SAH and considered indications for potential tertiary brain damage in cases of incomplete resolution.

Methods
Subarachnoid hemorrhage was induced through endovascular filament perforation in mice. Animals were killed 1, 7 and 14 days and 1, 2 and 3 months after SAH. Brain cryosections were immunolabeled for ionized calcium-binding adaptor molecule-1 to detect microglia/macrophages. Neuronal nuclei and terminal deoxyuridine triphosphate-nick end labeling staining was used to visualize secondary cell death of neurons. The gene expression of various proinflammatory mediators in brain samples was analyzed by quantitative polymerase chain reaction.

Results
We observed restored tissue homeostasis due to decreased microglial/macrophage accumulation and neuronal cell death 1 month after insult. However, the messenger RNA expression levels of interleukin 6 and tumor necrosis factor α were still elevated at 1 and 2 months post SAH, respectively. The gene expression of interleukin 1β reached its maximum on day 1, whereas at later time points, no significant differences between the groups were detected.

Conclusions
By the herein presented molecular and histological data we provide an important indication for an incomplete resolution of inflammation within the brain parenchyma after SAH. Inflammatory resolution and the return to tissue homeostasis represent an important contribution to the disease’s pathology influencing the impact on brain damage and outcome after SAH. Therefore, we consider a novel complementary or even superior therapeutic approach that should be carefully rethought in the management of cerebral inflammation after SAH. An acceleration of the resolution phase at the cellular and molecular levels could be a potential aim in this context.
Kulturell-ästhetisches Engagement von Kindern und Jugendlichen
Authors: Isenberg Tim , Thole Werner 
Published: June 2023

ZusammenfassungEinrichtungen der Kinder- und Jugendarbeit bieten in Kooperation mit externen Künstler_innen und der Stadt Frankfurt a. M. kulturell-ästhetische Workshops äußerst niedrigschwellig an, um Heranwachsenden die Partizipation an außerschulischer kultureller Bildung zu ermöglichen. Erste Ergebnisse zeigen die Möglichkeit nachhaltiger Wirkungseffekte und eine Förderung der sozialen, personalen und technischen Fertigkeiten. Während die Beziehung zwischen Kindern bzw. Jugendlichen und den Kunstschaffenden als ein zentraler Erfolgsfaktor identifiziert wurde, kämpfen die Einrichtungen insbesondere mit der Bereitstellung von Geldern und Räumlichkeiten, um kulturell-ästhetische Bildungsangebote auch nachhaltig in der Sozialen Arbeit implementieren zu können.
Intimate Partner Violence Directed at Men: Experiences of Violence, Help-seeking, and Potential Gender Role Conflict Among Portuguese Men
Authors: Reis Eduardo , Moleiro Carla , Arriaga Patrícia 
Published: June 2023

AbstractRecent research has shed light on how men in same-sex and different-sex relationships experience Intimate Partner Violence (IPV), and how it may contribute to their Gender Role Conflict (GRC), through which men face a dissonance with their gender roles. However, knowledge on these topics is still lacking. We aim to better understand men’s experiences of violence in their same-sex or different-sex relationships by identifying their dynamics and the specific barriers and facilitators to seeking help. Semi-structured interviews were conducted with 14 adult men (Heterosexual, n = 6; Gay, n = 5; Bisexual, n = 2; Not disclosed, n = 1) who self-identified as having been targets of IPV. The interview protocol considered the literature on IPV and the feedback from stakeholders in victim support services. The results were analyzed following the principles of Content Analysis. Our findings suggest that men are negatively impacted by their abusive relationships, with physical, psychological, sexual, and economic violence having been perpetrated by their same-sex or different-sex partners. Some men reported being further stigmatized in their help-seeking experiences. Gay and bisexual men indicated specific processes that played a significant role in their experiences. Some men revealed discomfort with the identity of “victim”, which may be linked to possible GRC processes. In sum, IPV impacts men in different-sex and same-sex relationships, and our findings provide new insights about how violence and help-seeking are experienced in the Portuguese cultural setting. Future efforts should focus on assessing possible GRC processes in this population and provide adequate information and tailored services to the specific needs of these men.
Schwerhörigkeit im Alter: Was steckt dahinter?
Authors: Köppl Christine , Klump Georg 
Published: June 2023

AbstractAge-related hearing loss is of increasing concern in our ageing society. However, a causal understanding of the manifold degenerative processes in the cochlea and auditory brain, and their relation to specific hearing problems, is still in its infancy. Gerbils that are trained to perform auditory tasks show very similar auditory perception to humans, including in tests with speech-like signals. This offers a unique opportunity to experimentally probe age-related changes in perception and the underlying neural pathologies.
Engineering biopharmazeutischer Produkte in der Pflanzenbiotechnologie
Authors: Eidenberger Lukas , Steinkellner Herta 
Published: June 2023

AbstractPlants can be used to recombinantly produce high-quality biopharmaceuticals, such as therapeutic proteins and vaccines, also known as plant molecular farming (PMF). Recent advances in engineering places PMF amongst the most innovative manufacturing technologies that reduce cost and shorten the production timeline. State-of-the-art PMF uses a dual-approach strategy to optimize the generation of products, namely engineering of expression vectors and production hosts. Here we describe the assembly of genetic circuits, engineered to enable the rapid expression of multimeric proteins in the commonly used expression host Nicotiana benthamiana.
Zugängliche KI-Algorithmen für bessere Zellmikroskopie
Authors: Mairhörmann Benedikt , Padovani Francesco , Schmoller Kurt M. 
Published: June 2023

AbstractArtificial intelligence (AI) has revolutionized many fields, including bioimage analysis. Powerful AI-based tools enable the analysis of large image datasets with unprecedented accuracy. However, easy-to-use tools are needed to allow experimentalists without specialised technical knowledge to leverage AI to its full potential. Here, we discuss our efforts to develop an intuitive and open-source bioimage analysis software that enables scientists to easily access state-of-the-art AI models.
Should young athletes be screened for cardiomyopathies to reduce the burden of sudden cardiac death in athletes?
Authors: McColgan Grace , Villarroel Mauricio , Gehmlich Katja 
Published: June 2023

AbstractIn this correspondence, we highlight the
risk of sudden cardiac death associated with undiagnosed cardiomyopathies. Life-threatening arrhythmias, which underlie sudden cardiac death, can be triggered by high-intensity exercise. It raises the question whether, and if so, how athletes should be screened for cardiomyopathies. The example of practice from Italy is discussed. We also briefly discuss novel developments, such as wearable biosensors and machine learning, which could be applied to screening for cardiomyopathies in future.
In-hospital outcomes after SAVR or TAVI in patients with severe aortic stenosis
Authors: Takeji Yasuaki , Taniguchi Tomohiko , Morimoto Takeshi , Shirai Shinichi , Kitai Takeshi , Tabata Hiroyuki , Kitano Kazuki , Ohno Nobuhisa , Murai Ryosuke , Osakada Kohei , Murata Koichiro , Nakai Masanao , Tsuneyoshi Hiroshi , Tada Tomohisa , Amano Masashi , Watanabe Shin , Shiomi Hiroki , Watanabe Hirotoshi , Yoshikawa Yusuke , Nishikawa Ryusuke , Yamamoto Ko , Obayashi Yuki , Toyofuku Mamoru , Tatsushima Shojiro , Kanamori Norio , Miyake Makoto , Nakayama Hiroyuki , Nagao Kazuya , Izuhara Masayasu , Nakatsuma Kenji , Inoko Moriaki , Fujita Takanari , Kimura Masahiro , Ishii Mitsuru , Usami Shunsuke , Sawada Kenichiro , Nakazeki Fumiko , Okabayashi Marie , Shirotani Manabu , Inuzuka Yasutaka , Ando Kenji , Komiya Tatsuhiko , Minatoya Kenji ,   on behalf of the CURRENT AS registry-2 Investigators, Kimura Takeshi 
Published: June 2023

Abstract
There were no data comparing the in-hospital outcomes after transcatheter aortic valve implantation (TAVI) with those after surgical aortic valve replacement (SAVR) in Japan. Among consecutive patients with severe AS between April 2018 and December 2020 in the CURRENT AS Registry-2, we identified 1714 patients who underwent aortic valve replacement (TAVI group: 1134 patients, and SAVR group: 580 patients). Patients in the TAVI group were much older (84.4 versus 73.6 years, P < 0.001) and more often had comorbidities than those in the SAVR group. In-hospital death rate was numerically lower in the TAVI group than in the SAVR group (0.6% versus 2.2%). After excluding patients with dialysis, in-hospital death rate was very low and comparable in the TAVI and SAVR groups (0.6% versus 0.8%). The rates of major bleeding and new-onset atrial fibrillation during index hospitalization were higher after SAVR than after TAVI (72% versus 20%, and 26% versus 4.6%, respectively), while the rate of pacemaker implantation was higher after TAVI than after SAVR (8.1% versus 2.4%). Regarding the echocardiographic data at discharge, the prevalence of patient-prosthesis mismatch was lower in the TAVI group than in the SAVR group (moderate: 9.0% versus 26%, and severe: 2.6% versus 4.8%). In this real-world data in Japan, TAVI compared with SAVR was chosen in much older patients with more comorbidities with severe AS. In-hospital death rate was numerically lower in the TAVI group than in the SAVR group.
Attitudes and Perception of the REFLECT Communication Curriculum for Clinical Oncology Graduate Medical Education
Authors: Laughlin Brady S. , Langley Natalie , Patel Samir H. , Kough Katherine , Ernst Brenda , Ashman Jonathan B. , Rule William G. , Vern-Gross Tamara Z. 
Published: June 2023

Abstract
Communication and interpersonal skills are essential components of oncology patient care. The REFLECT (Respect, Empathy, Facilitate Effective Communication, Listen, Elicit Information, Compassion, and Teach Others) curriculum is a novel framework to improve and refine physician/patient interactions for oncology graduate medical trainees. We seek to evaluate the attitudes and perceptions of the REFLECT communication curriculum among oncology trainees. Seven-question and 8-question Likert scale surveys (1 = not beneficial and 5 = beneficial) were distributed to resident/fellow participants and faculty mentors, respectively. Questions asked trainees and faculty about their perceptions of improvement in communication, handling of stressful situations, the value of the curriculum, and overall impression of the curriculum. Descriptive statistics determined the survey’s baseline characteristics and response rates. Kruskal–Wallis rank sum tests were used to compare the distribution of continuous variables. Thirteen resident/fellow participants completed the participant survey. Six (43.6%) Radiation Oncology trainees and 7 (58.3%) Hematology/Oncology fellows completed the trainee survey. Eight (88.9%) Radiation Oncologists and 1 (11.1%) Medical Oncologist completed the observer survey. Faculty and trainees generally felt that the curriculum increased communication skills. Faculty responded more favorably to the program’s impact on communication skills (median 5.0 vs. 4.0, p = 0.008). Faculty were more assertive about the curriculum’s capabilities to improve a learner’s ability to handle stressful situations (median 5.0 vs. 4.0, p = 0.003). Additionally, faculty had a more favorable overall impression of the REFLECT curriculum than the residents/fellows (median 5.0 vs. 4.0, p < 0.001). Radiation Oncology residents felt more strongly that the curriculum enhanced their ability to handle stressful topics, compared to Heme/Onc fellows (median 4.5 vs. 3.0, range 1–5, p = 0.379). Radiation Oncology trainees felt more consistently that the workshops improved their communication skills, compared to Heme/Onc fellows (median 4.5 vs. 3.5, range 1–5, p = 0.410). The overall impression between Rad Onc resident and Heme/Onc fellows was similar (median 4.0, p = 0.586). Conclusions: Overall, the REFLECT curriculum enhanced communication skills of trainees. Oncology trainees and faculty physicians feel that the curriculum was beneficial. As interactive skills and communication is critical to build positive interactions, further work is needed to improve the REFLECT curriculum.
Canis MitoSNP database: a functional tool useful for comparative analyses of human and canine mitochondrial genomes
Authors: Kowal Krzysztof , Tkaczyk-Wlizło Angelika , Jusiak Marcin , Grzybowska-Szatkowska Ludmiła , Ślaska Brygida 
Published: June 2023

AbstractCanis MitoSNP is a tool allowing assignment of each mitochondrial genomic position a corresponding position in the mitochondrial gene and in the structure of tRNA, rRNA, and protein. The main aim of this bioinformatic tool was to use data from other bioinformatic tools (TMHMM, SOPMA, tRNA-SCAN, RNAfold, ConSurf) for dog and human mitochondrial genes in order to shorten the time necessary for the analysis of the whole genome single nucleotide polymorphism (SNP) as well as amino acid and protein analyses. Each position in the canine mitochondrial genome is assigned a position in genes, in codons, an amino acid position in proteins, or a position in tRNA or rRNA molecules. Therefore, a user analysing changes in the canine and human mitochondrial genome does not need to extract the sequences of individual genes from the mitochondrial genome for analysis and there is no need to rewrite them into amino acid sequences to assess whether the change is synonymous or nonsynonymous. Canis mitoSNP allows the comparison between the human and canine mitochondrial genomes as well. The Clustal W alignment of the dog and human mitochondrial DNA reference sequences for each gene obtained from GenBank (NC_002008.4 dog, NC_012920.1 human) was performed in order to determine which position in the canine mitochondrial genome corresponds to the position in the human mitochondrial genome. This function may be useful for the comparative analyses. The tool is available at: https://canismitosnp.pl.
Association of albumin, globulin and albumin/globulin ratio with renal injury in type 2 diabetic nephropathy patients
Authors: Liu Dao-qin , Fu Xia , Yang Cheng-cheng , Zhou Ru , Zhao Hong-jing , Zhuang Ling-dan , Wu Qi-wen 
Published: June 2023

Abstract
Background
Studies have shown that albumin/globulin ratio (AGR) can assess the extent of kidney damage in type 2 diabetic nephropathy (T2DN). However, there is a lack of similar clinical data to support this.

Objectives
This study sought to inquire into the correlation of albumin (ALB), globulin (GLB) and albumin/globulin ratio (AGR) with renal injury in patients with type 2 diabetes mellitus (T2DM).

Methods
A retrospective analysis was performed on the clinical data of 82 patients with T2DM (Control group) and 110 patients with type 2 diabetic nephropathy (T2DN) who were admitted to the First Affiliated Hospital of Wannan Medical College from October 2019 to April 2022. T2DN patients were classified into mild renal impairment group (n = 75) and moderate renal impairment group (n = 35) according to urinary albumin excretion rate (UAER). Then, the general data of all groups were compared. Furthermore, Pearson correlation was used to analyze the correlation of serum ALB, GLB and AGR with UAER in the three groups. A receiver operating characteristic curve (ROC) was utilized to evaluate the diagnostic value of ALB, GLB and AGR for moderate renal injury in T2DN patients.

Results
There were significant differences in course of disease, history of hypertension, levels of fasting plasma glucose and glycosylated hemoglobin among the three groups. Besides, compared with the Control group, the levels of ALB and AGR were lower while GLB levels were higher in the mild and moderate renal impairment group. In particular, ALB and AGR levels were lower in the moderate renal impairment group relative to the mild renal impairment group, but the GLB levels exhibited no significant difference between the two groups. According to the results of Pearson correlation analysis, a negative correlation of ALB and AGR levels with UAER was revealed in T2DN patients. ROC curves displayed the area under the curve (AUC) of ALB (0.88) and AGR (0.71) predicting moderate renal injury in T2DM patients (p < 0.05). However, GLB has no significant diagnostic value for moderate renal injury in patients with T2DN.

Conclusion
The course of disease, hypertension and glycemic control may affect the occurrence and development of T2DN. ALB and AGR are of high value in predicting renal injury in patients with T2DN and can serve as the foundation for the clinical diagnosis of the condition.
Historiographical approaches to biogeography: a critical review
Authors: Bueno-Hernández Alfredo , Barahona Ana , J. Morrone Juan , Espinosa David , Juárez-Barrera Fabiola 
Published: June 2023

AbstractWe performed a critical review of the historiographical studies on biogeography. We began with the pioneering works of Augustin and Alphonse de Candolle. Then, we analyzed the historical accounts of biogeography developed by (1) Martin Fichman and his history on the extensionism-permanentism debate; (2) Gareth Nelson and his critique of the Neo-Darwinian historiography of biogeography; (3) Ernst Mayr, with his dispersalist viewpoint; (4) Alan Richardson, who wrote a microhistory on the biogeographic model constructed by Darwin; (5) Michael Paul Kinch and the ideas discussed in the 19th century about the geographical distribution of living beings; (6) Janet Browne, who highlighted the importance of the pre-Darwinian naturalists; (7) Peter Bowler, who focused mainly on the influence of paleontology on biogeography; (8) James Larson, who looked into the practices of the naturalists of Northern Europe in the late 18th century; and (9) Malte Ebach, who like Larson, was more interested in analysing the practices rather than the ideas of naturalists who studied the geographical distribution of organisms. Finally, these works are compared with each other. There has not been a dominant paradigm in the construction of historical narratives of biogeography; however, they provide a useful context for understanding problems of biogeography that continue to be debated to this day.
Spatial variation of physical and mechanical properties of tailings under different rainfall intensities and the interaction pattern
Authors: Nie Wen , Li Chenxi , Hu Jingwen , Saffari Pooya , Wang Wensong , Luo Minghua 
Published: June 2023

AbstractWith the continuous mining, a huge amount of mine waste has accumulated in tailing dams in China. The increasing frequency of abnormal rainfall in China poses a significant threat to the stability of tailings dams. It is important to study spatial variation of physical and mechanical properties of tailings for the process of dam destabilization due to rainfall. The Luomukeng tailings dam, Jiangxi province, China, was selected as the research object and the changes of physical parameters of tailings at different spatial locations under different rainfall intensities and influence on the tailing’s mechanical properties were studied. The correlations between the parameters were analyzed by Spearman correlation analysis. The results showed that rainfall led to particle migration, with a high number of fine particles repositioning. The greater the intensity of rainfall, the more significant the migration of particles, the more obvious the changes of physical and mechanical properties of tailings. Based on Spearman correlation analysis results, it was obtained that the change of inhomogeneity coefficient of tailings has the strongest effect on cohesion, and the change of water content has the strongest effect on friction angle.