bluuebunny/crossref_metadata_2025_split huggingface dataset:
Unearthing the Contextual Influence of National Culture on Entrepreneurship: Systematic Literature Review
Authors: Bate Adisu Fanta 
Published: January 2023

Abstract
The way national culture induces entrepreneurship and business growth remains contestable in the literature. Besides, little has been known about how national culture influences entrepreneurship across countries of different income categories. This paper, thus, aims to address how national cultural dimensions affect entrepreneurship, considering both developing and developed countries' contexts. The systematic literature review methodology is thoroughly pursued, and after identifying keywords and developing search queries, the Web of Science and EBSCO were utilized to trace out target studies. The review reveals individualism, indulgence, long-term orientation, low power distance, and low uncertainty avoidance culture are positively associated with entrepreneurship across countries. We postulate them as a set of pro-entrepreneurship cultural dimensions and argue that it is the bundling of these cultural dimensions that makes a difference in entrepreneurial performance, not an isolated effect of individual dimensions. The review also shows no clear distinction between developing and developed countries pertaining to national culture dimensions and their effect on entrepreneurship. It is wisely commendable to work on identifying and promoting the right set of cultural dimensions that can bolster entrepreneurship to enhance economic growth instead of on a particular dimension. The study claims to have significantly contributed new insights into the discourse on national culture and entrepreneurship.
Blocking microglial proliferation by CSF-1R inhibitor does not alter the neuroprotective effects of adoptive regulatory T cells in 3xTg Alzheimer's disease mice
Authors: Park Seon-Young , Cha Nari , Chae Songah , Lee Won-jun , Jung Hyun Jae , Bae Hyunsu 
Published: January 2023

Abstract
Background
Alzheimer's disease (AD) is a chronic neurodegenerative disease that causes cognitive impairment. Neuroinflammation induced by activated microglia exacerbates AD. Regulatory T cells (Tregs) play roles in limiting neuroinflammation by converting microglial polarization. Therefore, adoptive regulatory T cell therapy is considered an attractive option for neurodegenerative disorders. However, the mechanism underlying Treg therapy via microglial modulation is not fully understood. In this study, we sought to determine whether adoptively transferred Tregs were effective when microglia were depleted by CSF-1R inhibition.
Methods
First, we inhibited microglial proliferation using GW2580, a CSF-1R inhibitor, when Tregs were transferred. Learning and memory were assessed using a passive avoidance test. The accumulation of Aβ and pTAU, a hallmark of AD, was measured using immunofluorescence. Microglial neuroinflammation was assessed using immunofluorescence and RT-PCR. To track adoptively transferred Tregs, Tregs from Thy1.1 mice were transferred to 3xTg-AD Thy1.2 mice and mouse tissues, including brains, were harvested after 3–112 days.
Results
We found that inhibition of microglial proliferation during Treg transfer did not alter the therapeutic effects of Tregs on cognitive deficits and the accumulation of Aβ and pTAU in 3xTg-AD mice. The expression of pro- and anti-inflammatory markers in the hippocampus of 3xTg mice showed that GW2580 did not affect the inhibition of neuroinflammation by Treg transfer. Additionally, adoptively transferred Tregs were commonly detected in the brain on day 7 after transfer and their levels decreased slowly over 100 days.
Conclusions
Together, these data suggest that adoptively transferred Tregs can survive longer than 100 days in the brain, suppressing microglial activation and thus alleviating AD pathology. The present study provides valuable evidence to support the prolonged efficacy of adoptive Treg therapy in AD.
Low temperature phenol-degrading microbial agent: construction and mechanism
Authors: Yang Yu , Zhang Yu , Liu Cong , Su Zhiqiang , Zhao Ruizhi , Zhou Jiti 
Published: January 2023

Abstract
In this study, three cold-tolerant phenol-degrading strains, Pseudomonas veronii Ju-A1 (Ju-A1), Leifsonia naganoensis Ju-A4 (Ju-A4), and Rhodococcus qingshengii Ju-A6 (Ju-A6), were isolated. All three strains can produce cis, cis-muconic acid by ortho-cleavage of catechol at 12 ℃. Response surface methodology (RSM) were used to optimize the proportional composition of low-temperature phenol-degrading microbiota. Degradation of phenol below 160 mg L-1 by low-temperature phenol-degrading microbiota followed first-order degradation kinetics. When the phenol concentration was greater than 200 mg L-1, the overall degradation trend was in accordance with the modified Gompertz model. The experiments showed that the bacterial agent (three strains of low-temperature phenol-degrading bacteria were fermented separately and constructed in the optimal ratio) degraded phenol at the fastest rate. The above construction method is more advantageous in the actual wastewater treatment.
WHEAT LEAF RUST DETECTION BASED ON MULTI-SCALE DWT AND LCS COLOR THRESHOLDING METHODS
Authors: Khan Sajid Ullah , Ullah Imdad , Rehman Alia , Khan Fahim , Lee youngmoon 
Published: January 2023

Abstract
Health, Technology, education, and food production are the four main issues facing developing nations like Pakistan, and it is undeniable that agriculture is the most important factor behind economic growth. In addition, implementing a strategy for food production is crucial for citizens to ensure their survival, and it is assumed that these initiatives will result in sufficient farm productivity. One strategy to make a field productive is to take significant care of its components, which starts with cultivating healthy plants or crops. Wheat leaf rust is a fatal condition that attacks young seedlings. It is a significant fungi disease. Leaf rust has 25% effect on the productivity of wheat. To mitigate this issue, a Multi-Scale Discrete Wavelet Transform (MsclDWT) using hybrid fusion rules method is proposed to obtain the complementary information from multiple input images. In second phase, Lab color space followed by color thresholding method is applied to detect and segment wheat leaf rust disease in wheat crop. The proposed model also computes the rust-affected area of the wheat crop, which assists the farmers in the post-medication (anti rust spray) process. The empirical results show that the proposed model achieved 97% of accuracy in rusted pixels detection and classification and outperformed the existing comparative methods.
High proprotein convertase subtilisin/kexin type 9 antibody level is associated with poor prognosis in patients with diabetes: A Prospective cross-sectional study
Authors: Yamagata Hiroki , Hayashi Aiko , Yoshida Yoich , Koshizaka Masaya , Onishi Shunichiro , Yohida Tomohiko , Hiwasa Takaki , Takemoto Minoru 
Published: January 2023

Abstract
Background
In addition to pathogenic autoantibodies, polyclonal autoantibodies with unknown physiological roles and pathogenicity are produced in the body. Moreover, serum antibodies against the proprotein convertase subtilisin/kexin type 9 (PCSK9) protein, which is deeply involved in cholesterol metabolism, have also been observed. Therefore, we examined the clinical significance of PCSK9 antibody (PCSK9-Ab) levels.
Methods
We measured the blood PCSK9-Ab and PCSK9 protein levels in 109 healthy donors (HD) and 274 patients with diabetes mellitus (DM) (type 2 DM: 89.8%) using an amplified luminescence proximity homogeneous assay-linked immunosorbent assay. Subsequently, patients with DM were followed up (mean: 4.93 years, standard deviation: 2.77 years, maximum: 9.58 years, minimum: 0.07 years) to examine associations between antibody titers and mortality, myocardial infarction, stroke onset, and cancer.
Results
Although PCSK 9-Ab levels were significantly higher in the DM group than in the HD group, PCSK9 protein levels were not significantly different. PCSK9-Ab and PCSK9 protein levels showed no correlation in either group. We found that mortality was significantly associated with higher PCSK9-Ab levels, but not related to PCSK9 protein levels. After investigating for potential confounding factors, PCSK9-Ab levels were significantly associated with prognosis in patients with DM.
Conclusions
PCSK9-Ab may be a novel prognostic marker in patients with diabetes. However, further studies are warranted to verify its usefulness as a prognostic marker.
Berberine exhibits antitumor effects by activating autophagy and apoptosis in anaplastic thyroid carcinoma cells by regulating ROS and the PI3K/AKT/mTOR signaling pathways
Authors: Shi Xiang-Zhe , Zhao Sheng , Wang Yan , Wang Meng-Yao , Xiong Chen , Su Su-Wen , Wu Yan-Zhao 
Published: January 2023

AbstractAnaplastic thyroid carcinoma (ATC) is the most lethal thyroid carcinoma. Doxorubicin (DOX) is the only drug approved for anaplastic thyroid cancer treatment, but its clinical use is restricted due to irreversible tissue toxicity. Berberine (BER), an isoquinoline alkaloid extracted from Coptidis Rhizoma, has been proposed to have antitumor activity in many cancers. However, the underlying mechanisms by which BER regulates apoptosis and autophagy in ATC remain unclear. Thus, the present study aimed to assess the therapeutic effect of BER in CAL-62 and BHT-101 cells as well as the underlying mechanisms. In addition, we assessed the antitumor effects of a combination of BER and DOX in ATC cells. The present results showed that BER significantly inhibited cell growth and induced apoptosis in ATC cells. BER treatment also significantly upregulated the expression of LC3B-II and increased the number of GFP-LC3 puncta in ATC cells, suggesting that BER induced a high level of autophagy. Inhibition of autophagy by 3-methyladenine (3-MA) suppressed BER-induced autophagic cell death, which confirmed the anticancer role of autophagy induced by BER. Moreover, BER induced the generation of reactive oxygen species (ROS). N-acetylcysteine (NAC), a potential ROS scavenger, substantially suppressed the expression of autophagy-regulated proteins and apoptosis proteins induced by BER. Moreover, BER and DOX cooperated to promote apoptosis and autophagy in ATC cells. Mechanistically, we demonstrated that BER regulated the autophagy and apoptosis of human ATC cells through the PI3K/AKT/mTOR pathways. Taken together, the present findings indicated that BER induces apoptosis and autophagic cell death by activating ROS and regulating the PI3K/AKT/mTOR signaling pathway.
Consolidative breast radiotherapy and prophylactic high-dose methotrexate are important first-line treatments for primary breast diffuse large B-cell lymphoma patients treated with R-CHOP-like regimens
Authors: Weng Huawei , Shrestha Prem Raj , Chen Zegeng , Hong Huangming , Huang He , Yu Le , Yao Yuyi , Li Xiaoqian , Pan Fei , Zhang Wei , Chen Yongchang , Li Xudong , Wan Mengdi , Zhang Zhihui , Zou Liqun , Zhu Bo , Zhou Hui , Liu Xianling , Liu Yao , Guo Hongqiang , Fang Xiaojie , Wang Zhao , Lin Tongyu 
Published: January 2023

Abstract
Purpose
Primary breast diffuse large B-cell lymphoma (PB-DLBCL) is a rare form of extranodal DLBCL. In this study, we aimed to determine the patterns of relapse and the optimal treatment strategy for PB-DLBCL in the rituximab era.
Methods
We retrospectively collected data from Chinese Southwest Oncology Group-affiliated institutes. Patients diagnosed with PB-DLBCL from 2008 to 2019 and treated with rituximab plus cyclophosphamide, doxorubicin, vincristine, prednisone (R-CHOP) or R-CHOP-like regimens were included.
Results
A total of 135 PB-DLBCL patients treated with R-CHOP or R-CHOP-like regimens were eligible for this study. With a median follow-up of 43 months, the 5-year overall survival (OS) and progression-free survival (PFS) rates were 84.7% and 69.6%, respectively. Continuous treatment failure was observed, especially affecting the breast and central nervous system (CNS). Consolidative RT significantly reduced the risk of breast relapse (p = 0.013). Relapse in CNS were detected in 13 (9.6%) patients, of whom 4 had received intrathecal prophylaxis and 9 had not received CNS prophylaxis. None of the patients who received high-dose methotrexate (HD-MTX) had CNS relapse. CNS relapse risk was reduced by HD-MTX (p = 0.036). Furthermore, we screened the genetic mutation profile of PB-DLBCL and found that MYD88 and/or CD79B mutations were present in all patients with CNS relapse, whereas patients with MYD88 and/or CD79B mutations who received HD-MTX did not experience CNS relapse.
Conclusions
Our results indicate that consolidative RT decreased the risk of breast relapse. Prophylactic HD-MTX reduced the risk of CNS relapse, especially in patients with MYD88/CD79B mutations.
Numerical simulation on melt pool and solidification in the direct energy deposition process of GH3536 powder superalloy
Authors: Liu Miao , Liu Zhongqiu , Li Baokuan , Qi Fengsheng , Peng Weiping 
Published: January 2023

Abstract
In the direct energy deposition (DED) process, the highly energetic laser, rapid melting, and solidification processes lead to complex heat transfer and flow phenomena. A three-dimensional finite element model (FEM) is established to study the effect of process parameters on the melt pool and solidification quality during the DED process. The heat transfer, fluid flow, and solidification in the DED process of the GH3536 superalloy are studied. By investigating the effects of laser power, scanning speed, and feed rate on the morphology of melt pool and interlayer fusion, the appropriate input parameters for GH3536 are obtained. Temperature gradient and solidification rate obtained in transient thermal distribution are applied to predict the quality and morphology of the solidified structure at the cut-off point. Results show that high laser power and low scanning speed or feed rate will enlarge the melt pool. Well-solidified microstructure frequently appears in the middle of the parameter set; focusing on the enlargement of the melt pool is not the best strategy. The correlation between feed rate and laser power is not obvious. The minimum threshold for scanning speed is found at a given feed rate. When the scanning rate is below the threshold, abnormal morphology of the melt pool and irregular solidification structures will occur. The laser power and scanning speed range suitable for the GH3536 superalloy are summarized, and the undesirable and possibly fluctuating parameters are marked. The middle part of the parameter set is recommended for the feed rate.
Medicago truncatula-Sinorhizobium meliloti-Fusarium oxysporum tripartite interaction alters nodulation and nitrogen fixation
Authors: BATNINI Marwa , López-Gómez Miguel , Hidalgo-Castellanos Javier , Palma Francesco , Peña Agustín Javier Marín , Mhadhbi Haythem 
Published: January 2023

Abstract
Plants encounter a myriad of microorganisms at the root-soil interface that can invade with detrimental or beneficial outcomes. Promoting a symbiotic relationship may interfere with the restriction responses to pathogens. In this study, we established a tripartite Medicago truncatula- Sinorhizobium meliloti-Fusarium oxysporum interaction to study the effect of the interplay between symbiosis and defense on the symbiosis establishment and efficiency in a resistant (A17) and susceptible (TN1.11) genotype to F. oxysporum infection. Our results showed that Sm induced the expression of allene oxide cyclase 1 and 2 (MtAoc1) and (MtAoc2) at 6 and 24 hours post infection (hpi) in A17 and at 24 hpi in TN1.11. This up-regulation of MtAoc1 and MtAoc2 was also observed when co-inoculating TN1.11 genotype with Fo and Sm. Moreover, in this later treatment the expression level of the gene of the common signaling pathway doesn’t make infection 3 (DIM3) was reduced compared to Sm-inoculated plants. This reduction of DIM3 transcripts was concomitant to a decrease in nodule number (NN), nodule fresh weight, and nitrogen-fixing rate (NFR), and to an accumulation of spermine in nodules. This defectiveness in symbiosis parameters, in the co-inoculation treatment, was also observed in A17 genotype, along with an accumulation of phytohormones in leaves and polyamines in nodules. Our results suggest that in the tripartite interaction, the high expression of MtAoc and the decrease of DMI3 transcripts along with the accumulation of phytohormones and polyamines in leaves overlapped with symbiosis establishment and functioning and thus, reduced NN and NFR.
Curative resection following conservative treatment for perforated early gastric cancer: A case report
Authors: Yoshizawa Junichi , Sugiyama Satoshi , Kubota Koji , Nakayama Ataru 
Published: January 2023

Abstract
Background: Gastric perforation is a relatively rare complication of gastric cancer. Malignant gastric perforation is often a manifestation of advanced cancer with serosal invasion and lymph node metastasis; thus, gastric cancer rarely perforates at an early stage. Herein, we describe a case of gastric perforation, which was treated conservatively; later, the patient was diagnosed with an extremely rare early-stage gastric cancer and underwent radical surgery in two stages.
Case presentation: An 81-year-old woman was referred to our hospital for breathlessness. Abdominal contrast-enhanced computed tomography (CT) revealed gastric perforation and perforated peritonitis. She was hospitalized, and conservative treatment was initiated; upper gastrointestinal endoscopy was performed on the 14th day after admission. Gastric ulcer scars were observed in the anterior wall of the stomach; biopsy from around the ulcer showed group V (moderately-well differentiated adenocarcinoma). She was diagnosed with gastric perforation due to early gastric cancer. Abdominal CT revealed no findings suggestive of liver, lung, or para-aortic lymph node metastasis, and a radical gastrectomy with lymph node dissection was performed 32 days after onset. Microscopic findings revealed that the cancer cells proliferated to the regenerated mucosa of ulcer scars and infiltrated into scar tissue equivalent to submucosal tissue (T1b). The gastric cancer was staged as T1bN0M0, stage IA. The patient has been reported healthy without any evidence of gastric cancer recurrence for the past 48 months.
Conclusions: Treatment of gastric cancer perforation includes first-stage surgery and second-stage surgery after conservative therapy and closure of the omental patch or perforation site. Ensuring a balance between lifesaving and curability, while selecting surgical methods is necessary. Depending on the patient's general condition and degree of peritonitis, curatively resectable perforated gastric cancer, including early-stage gastric cancer, should be treated conservatively as in our case, or minimally invasively with an omental patch or perforation closure as surgery for gastric perforation. After evaluation of the patient's general condition and adequate assessment of the extent and progression of the gastric cancer, a two-stage gastrectomy with lymph node dissection was suitable and achieved an improved general condition, which may lead to a highly curative surgery and improve prognosis.
Detection and Mitigation of Malicious DDoS Floods in Software Defined Networks
Authors: Ahmad Furqan 
Published: January 2023

Abstract
Software-defined networking provides modular network management, allowing the flexible quality of services to remove conventional networks' limitations. It implies the concept of separating the control and data plane attributes for flexible network management. Contrary to network flexibilities, the centralized management is exposed to cyber threats i.e., Distributed Denial-of-service (DDoS) attacks which can compromise of SDN controllers. Meanwhile, entropy-based DDoS attack detection methods are most prominent among other detection methods but relying on entropy itself can neglect detection in several parameters i.e., variations in flow specification. In this research, a DDOS attacks detection and mitigation framework inside the SDN control plane is designed to ensure the secure availability of the network. Our approach comprises an entropy-based detection system integrated flow initiation and specification modules to classify the malicious DDoS flows against regular traffic. This lightweight approach is designed to minimize DDoS attacks by detecting its effects in the early stages a perform mitigation before compromising the controller resources. The simulation is performed on Mininet network simulator, for implementing SDN architecture and the testbed is created on various DDOS attacks, i.e., UDP, TCP-SYN, and ICMP ping flood attacks, to validate on commonly used data centric network environments. Based on the results, the proposed solution assures the SDN-based DDoS attack detection and mitigation under 150 packets maintaining significantly low detection time and high accuracy.
Combination of Astragaloside II and Notoginsenoside Fc Ameliorated Apoptosis and Mitochondrial Oxidative Stress in Diabetes with Acute Kidney Injury via activating PDGFB and inhibiting SHP-1/VEGFA pathway
Authors: Shen Yilan , Ye Dan , Lin Kanghong , Guo Xieyi , Wang Niansong , Xin Wenfeng , Xu Youhua , Gui Dingkun 
Published: January 2023

Abstract
Acute kidney injury (AKI) is a disease with high morbidity and mortality and ischemia-reperfusion (IR) injury is the main cause of AKI. It has been reported that hyperglycemia was a crucial risk factor for renal IRI in diabetes patients with renal IR-induced AKI. Whereas there is no effective treatment. Here, we explored the nephroprotective effects of combination of Astragaloside II and Notoginsenoside Fc on diabetes with IR-induced AKI and its potential mechanisms. We established hyperglycemia with renal hypoxia reoxygenation (H/R) injury model in vivo and in vitro. We first found that combination of Astragaloside II and Notoginsenoside Fc could improve biochemical indexes and renal histologic injury in model mice. We provided relevant evidence that combination of Astragaloside II and Notoginsenoside Fc significantly reduced apoptosis level. In addition, our data suggested that combination of Astragaloside II and Notoginsenoside Fc could have inhibited effects on oxidative stress and NLRP3 activation, further suppressing inflammatory cell aggregation and inflammatory factor secretion. Further studies found that the combination of Astragaloside II and Notoginsenoside Fc could prevent SHP-1-induced VEGF inhibition and activate PDGFB to ameliorate renal tubular epithelial cells (TECs) function. In conclusion, our study indicated that combined treatment of Astragaloside II and Notoginsenoside Fc exerted beneficial protective effects on renal tubular injury and mitochondrial oxidative stress in diabetes with ischemia-reperfusion induced AKI via activating PDGFB and inhibiting SHP-1/VEGFA signaling pathway. Therefore, combination of Astragaloside II and Notoginsenoside Fc may be a potential therapeutic strategy to treat diabetes with IR-induced AKI.