bluuebunny/crossref_metadata_2025_split huggingface dataset:
Priority setting to support a public health research agenda: a modified Delphi study with public health stakeholders in Germany
Authors: Hoekstra Dyon , Gerhardus Ansgar , Lhachimi Stefan K. 
Published: August 2023

Abstract
Background
Research priority setting (RPS) studies are necessary to close the significant gap between the scientific evidence produced and the evidence stakeholders need. Their findings can make resource allocation in research more efficient. However, no general framework for conducting an RPS study among public health stakeholders exists. RPS studies in public health are rare and no such study has been previously conducted and published in Germany. Therefore, we aimed to investigate which research topics in public health are prioritised by relevant stakeholders in Germany.

Methods
Our RPS study consisted of a scoping stage and a Delphi stage each split into two rounds. Firstly, we invited members of the German Public Health Association to gather expert insights during two initial workshops. Next, we defined the relevant stakeholder groups and recruited respondents. Thereafter, we collected research topics and assessment criteria with the respondents in the first Delphi round and aggregated the responses through content analysis. Finally, we asked the respondents to rate the research topics with the assessment criteria in the second Delphi round.

Results
In total, 94 out of the 140 invited public health organisations nominated 230 respondents for the Delphi study of whom almost 90% participated in both Delphi rounds. We compiled a comprehensive list of 76 research topics that were rated and ranked by several assessment criteria. We split the research topics into two types, substantive research topics and methodological-theoretical research topics respectively, to ensure the comparability among the research topics. In both types of research topics—substantive research topics and methodological-theoretical research topics—the respective top five ranked research topics hardly differed between public health researchers and public health practitioners. However, clear differences exist in the priority ranking of many (non-top priority) research topics between the stakeholder groups.

Conclusions
This research demonstrates that it is possible, with limited resources, to prioritise research topics for public health at the national level involving a wide range of pertinent stakeholders. The results can be used by research funding institutions to initiate calls for research projects with an increased relevance for health and/or scientific progress.
Rapid and accurate genotyping of human SNP rs671 in aldehyde dehydrogenase 2 gene using one-step CRISPR/Cas12b assay without DNA amplification
Authors: Wu Fang , Xue Yong , Wang Yan , Si Xinxin , Zhang Xinyue , Xu Yuyang , Luo Zhidan 
Published: August 2023

Abstract
Background
The SNP rs671 of Human aldehyde dehydrogenase (ALDH) is G-A transition at 1510th nucleotides, which is an important clinical indicator of alcoholic liver disease, digestive tract cancer and some drug efficiency. The commonly used genotyping assay of this polymorphism is relatively time-consuming and costly.

Finding
This study develops a rapid and accurate one-step CRISPR/Cas12b assay to distinguish the G1510A polymorphism of human ALDH2 free of DNA amplification. The method we established requires only one step of adding 1 μl genomic DNA sample to premixed system, and waiting for the acquisition of fluorescent signal, taking approximate 30 min.

Conclusions
This method provides a potential tool for more accurate and reliable nucleic acid detection with a single base difference and supports the relevant disease diagnosis and personalized medicine.
Evaluation of calcification distribution by CT-based textural analysis for discrimination of immature teratoma
Authors: Nakamori Akari , Tsuyoshi Hideaki , Tsujikawa Tetsuya , Orisaka Makoto , Kurokawa Tetsuji , Yoshida Yoshio 
Published: August 2023

Abstract
Background
Mature and immature teratomas are differentiated based on tumor markers and calcification or fat distribution. However, no study has objectively quantified the differences in calcification and fat distributions between these tumors. This study aimed to evaluate the diagnostic potential of CT-based textural analysis in differentiating between mature and immature teratomas in patients aged < 20 years.

Materials and methods
Thirty-two patients with pathologically proven mature cystic (n = 28) and immature teratomas (n = 4) underwent transabdominal ultrasound and/or abdominal and pelvic CT before surgery. The diagnostic performance of CT for assessing imaging features, including subjective manual measurement and objective textural analysis of fat and calcification distributions in the tumors, was evaluated by two experienced readers. The histopathological results were used as the gold standard. The Mann–Whitney U test was used for statistical analysis.

Results
We evaluated 32 patients (mean age, 14.5 years; age range, 6–19 years). The mean maximum diameter and number of calcifications of immature teratomas were significantly larger than those of mature cystic teratomas (p < 0.01). The mean number of fats of immature teratomas was significantly larger than that of mature cystic teratomas (p < 0.01); however, no significant difference in the maximum diameter of fats was observed. CT textural features for calcification distribution in the tumors showed that mature cystic teratomas had higher homogeneity and energy than immature teratomas. However, immature teratomas showed higher correlation, entropy, and dissimilarity than mature cystic teratomas among features derived from the gray-level co-occurrence matrix (GLCM) (p < 0.05). No significant differences were observed in the CT features of fats derived from GLCM.

Conclusion
Our results demonstrate that calcification distribution on CT is a potential diagnostic biomarker to discriminate mature from immature teratomas, thus enabling optimal therapeutic selection for patients aged < 20 years.
Stability of guayulins from Parthenium argentatum, A. Gray during post-harvesting storage for industrial exploitation
Authors: Latorre Guayente , García-Martínez M. Mercedes , Coello Juana , Carrión María Engracia , Zalacain Amaya , Carmona Manuel 
Published: August 2023

Abstract
Background
Guayulins comprise a family of sesquiterpene compounds with potential industrial applications that are extracted from the resinous fraction of the guayule plant (Parthenium argentatum, A. Gray). If a whole industry is to be developed around guayulins, not only their activity should be assessed, but also their stability because the quality of the final products is likely influenced by the time that elapses from harvesting to processing. Thus, the aim of the present study was to evaluate the stability of guayulin compounds from harvested guayule stems or extracted resin after storage at different temperatures to find in which form, stems or resin, is the storage better.

Results
Results showed that, once extracted, the resin could be stored at 20-25 °C for 3 months or more without significant losses of guayulin content. In the case of harvested stems, however, the findings were more complex, with guayulins A and B degrading over time and guayulins C and D showing enrichment. In addition, analysis of the thermal and thermo-oxidative degradation of the resin and guayulins showed that while guayulins A and B showed a maximum decomposition rate around 280 °C, guayulins C and D decomposed at 245 °C. Such thermal differences might be attributed to the observed oxidation of guayulin A and B standards into guayulin C and D, respectively.

Conclusion
These findings provide, for the first time, information on the stability of guayulins after harvesting and resin extraction, which could help to the development of an integral industrial process from harvesting to commercializing.

Graphical Abstract
Deep learning approximation of attenuation maps for myocardial perfusion SPECT with an IQ$$\varvec{\cdot {}}$$SPECT collimator
Authors: Huxohl Tamino , Patel Gopesh , Zabel Reinhard , Burchert Wolfgang 
Published: August 2023

Abstract
Background
The use of CT images for attenuation correction of myocardial perfusion imaging with single photon emission computer tomography (SPECT) increases diagnostic confidence. However, acquiring a CT image registered to a SPECT image is often not possible because most scanners are SPECT-only. It is possible to approximate attenuation maps using deep learning, but this has not yet been shown to work for a SPECT scanner with an IQ$$\varvec{\cdot {}}$$

·

SPECT collimator. This study investigates whether it is possible to approximate attenuation maps from non-attenuation-corrected (nAC) reconstructions acquired with a SPECT scanner equipped with an IQ$$\varvec{\cdot {}}$$

·

SPECT collimator.

Methods
Attenuation maps and reconstructions were acquired retrospectively for 150 studies. A U–Net was trained to predict attenuation maps from nAC reconstructions using the conditional generative adversarial network framework. Predicted attenuation maps are compared to real attenuation maps using the normalized mean absolute error (NMAE). Attenuation-corrected reconstructions were computed, and the resulting polar maps were compared by pixel and by average perfusion per segment using the absolute percent error (APE). The training and evaluation code is available at https://gitlab.ub.uni-bielefeld.de/thuxohl/mu-map.

Results
Predicted attenuation maps are similar to real attenuation maps, achieving an NMAE of 0.020±0.007. The same is true for polar maps generated from reconstructions with predicted attenuation maps compared to CT-based attenuation maps. Their pixel-wise absolute distance is 3.095±3.199, and the segment-wise APE is 1.155±0.769.

Conclusions
It is feasible to approximate attenuation maps from nAC reconstructions acquired by a scanner with an IQ$$\varvec{\cdot {}}$$

·

SPECT collimator using deep learning.
Morphology of the male reproductive system and sperm ultrastructure of the green lacewing, Chrysopa pallens (Rambur, 1838) (Neuroptera: Chrysopidae)
Authors: Liu Xiaoping , Guo Xingkai , Feng Yanjiao , Zhang Lisheng , Wang Mengqing , Li Yuyan , Mao Jianjun 
Published: August 2023

Abstract
Background
Chrysopa pallens is one of the most beneficial and effective natural predators, and is famous for its extensive distribution, wide prey spectrum, and excellent reproductive performance. This study examined the anatomy and fine structure of the C. pallens reproductive system and spermatogenesis.

Results
The male reproductive system of C. pallens comprises a pair of testes, a vas deferens, seminal vesicles, accessory glands, and short ejaculatory ducts. The testes were already mature on the day of emergence, but the accessory glands did not mature until 5 days post-emergence. In early spermatids, the flagellum had an axoneme on one side of the two mitochondrial derivatives. The nucleus was surrounded by parallel crystalline and paracrystalline materials. The spermatid envelope extends towards the paracrystalline material in a tail-shaped wing. In mature spermatids, the axoneme is located between the two accessory bodies and mitochondrial derivative sets. The parallel-crystalline and paracrystalline materials disappeared. In the testes, the wall of seminal cysts consists of a layer of epithelium, a muscular-connective sheath, and several vesicles of different sizes. The mature seminal cysts contained 128 spermatozoa. The accessory gland is composed of six parts: ventral papilla-like protuberance, anterior glandular lobe, lateral glandular lobe, seminal cyst, posterior kidney-shaped lobe, and posterior papilla-like protuberance. Muscle fibers and secretory granules are extensive.

Conclusions
This study provides information on the reproductive system of C. pallens and offers a resource for taxonomy and reproductive biology.
Circular developments of maritime industrial ports in Europe: a semi-systematic review of the current situation
Authors: Barona J. , Ballini F. , Canepa M. 
Published: August 2023

AbstractOur current approaches to resource management are not sustainable. Businesses typically follow a linear approach where resources are not well utilized. Maritime ports are part of the problem, as they need to optimize the use of resources and generate negative externalities. However, they can also be important drivers of sustainability if they effectively transition to Circular Economy (CE). In this study, eight principles of CE and ten CBMs, are discussed and used to analyze ports in three areas of intervention. This paper analyzes current CE practices in European ports and the potential to adopt Circular Business Models (CBMs) to create value for port stakeholders and contribute to the United Nations Sustainable Development Goals (SDGs). Drivers and barriers to CE were analyzed, and the level of development was assessed. The ports studied are in Europe and are generally regarded as Maritime Industrial Ports (MIP). The methodology includes a Semi-Systematic Literature Review (SSLR) and a SWOT analysis to examine CE practices in maritime ports. This methodology helped enhance the analysis of Drivers and Barriers to CE transition. This study concludes that ports are developing circular practices and business models on the technical and biological flows, but the level of implementation is moderated to low. Six drivers for adopting CE principles were identified, including European ports’ experience in circular activities and the Quality & Environmental Management System (EMS). In addition, seven barriers to CE were highlighted, including the diversity of port characteristics and the different interests and ambitions of port stakeholders that make it difficult to standardize measurements and transition to circularity. Circular development goals in ports should be integrated, and development must balance social, economic, and environmental sustainability. The findings and implications of this study will help Port Authorities (PA) in the strategy, policy development, and implementation of CBMs.
The priority areas and possible pathways for health cooperation in BRICS countries
Authors: Liu Zuokun , Wang Zongbin , Xu Ming , Ma Jiyan , Sun Yinuo , Huang Yangmu 
Published: August 2023

AbstractAs one of the largest alliances of middle-income countries, the BRICS, known as an acronym for five countries including “Brazil, Russia, India, China, and South Africa”, represents half of the global population. The health cooperation among BRICS countries will benefit their populations and other middle- and low-income countries. This study aims to summarize the current status of health cooperation in BRICS countries and identify opportunities to strengthen BRICS participation in global health governance. A literature review was conducted to analyze the status, progress, and challenges of BRICS' health cooperation. Content analysis was used to review the 2011–2021 annual joint declarations of the BRICS Health Ministers Meetings. The priority health areas were identified through segmental frequency analysis. Our research suggested that communicable diseases, access to medicine, and universal health coverage appeared most frequently in the content of declarations, indicating the possible top health priorities among BRICS' health collaboration. These priority areas align with the primary health challenges of each country, including the threats of double burden of diseases, as well as the need for improving health systems and access to medicines. Respective external cooperation, inter-BRICS health cooperation, and unified external cooperation are the main forms of health cooperation among BRICS countries. However, challenges such as the lack of a unified image and precise position, lack of practical impact, and weak discourse power have impeded the impact of BRICS on health governance. This study suggests that the BRICS countries should recognize their positioning, improve their unified image, and establish cooperative entities; at the same time, they should increase their practical strength, promote non-governmental cooperation, and expand the cooperation space through the “BRICS Plus” mechanism with countries with similar interests to join.
Baricitinib improves pulmonary fibrosis in mice with rheumatoid arthritis-associated interstitial lung disease by inhibiting the Jak2/Stat3 signaling pathway
Authors: Liu Hongli , Yang Yan , Zhang Jie , Li Xuelin 
Published: August 2023

Abstract
Objective
The study explored improvements in pulmonary inflammation and fibrosis in a bovine type II collagen-induced rheumatoid arthritis-associated interstitial lung disease mouse model after treatment with baricitinib and the possible mechanism of action.

Methods
A rheumatoid arthritis-associated interstitial lung disease mouse model was established, siRNA Jak2 and lentiviral vectors were transfected with human embryonic lung fibroblast cells. And the levels of relevant proteins in mouse lung tissue and human embryonic lung fibroblasts were detected by Western blotting.

Results
The levels of JAK2, p-JAK2, p-STAT3, p-SMAD3, SMA, TGFβR2, FN and COL4 were increased in the lung tissues of model mice (P < 0.5) and decreased after baricitinib intervention (P < 0.05). The expression levels of p-STAT3, p-SMAD3, SMA, TGFβR2, FN and COL4 were reduced after siRNA downregulation of the JAK2 gene (P < 0.01) and increased after lentiviral overexpression of the JAK2 gene (P < 0.01).

Conclusion
Baricitinib alleviated fibrosis in the lung tissue of rheumatoid arthritis-associated interstitial lung disease mice, and the mechanism of action may involve the downregulation of Smad3 expression via inhibition of the Jak2/Stat3 signaling pathway, with consequent inhibition of the profibrotic effect of transforming growth factor-β1.
A comparative study between the system reliability evaluation methods: case study of mining dump trucks
Authors: Moniri-Morad Amin , Sattarvand Javad 
Published: August 2023

AbstractThe shovel-truck system is a widely used technique for haulage systems in surface mining operations. However, predicting the failure patterns of complex systems requires accurate failure prediction techniques. In this study, several major system reliability evaluation groups, including non-parametric, parametric, and semi-parametric methods, are investigated, and their effectiveness is compared to identify the best group for predicting the failure patterns of complex systems such as mining dump trucks, which operate in harsh environments. A historical dataset of time to failure (TTF) and maintenance data was collected. Then, the system’s reliability was evaluated using the major TTF data analysis methods. The findings demonstrated that all the major system reliability evaluation groups produced similar curves; however, the semi-parametric method outperformed the other methods. This result underscores that this system reliability evaluation group is the most effective method for complex systems. Also, it was found that the dump truck reliability dropped to 50% after 40 operation hours, demonstrating the critical importance of implementing preventive maintenance to enhance the system’s performance and ensure operation safety. In addition, this study provided an appropriate insight into the predictive methods and offered an accurate estimation of the failure pattern of complex systems, resulting in availability and productivity improvement.
Inovações da legislação tributária no período de 1974/75
Authors: Dornelles Francisco Oswaldo Neves 
Published: August 2023

1. A Emenda nº 18, de 1965. 2. Imposto sobre circulação de mercadorias.3. A Lei Complementar nº 24, de 1975. 4. Imposto sobre produtos industrializados. 5. Imposto de importação. 6. Imposto de renda. 7. Fundos de investimentos. 8. Acordos internacionais. 9. O Deecreto-lei nº 1 380. 10. Conclusão.
Funcionário público - Remuneração - Cotas e percentuais - Gratificação de produtividade
Authors: Silva Carlos Medeiros 
Published: August 2023

- Com o propósito de manter o nível de rendimento, o legislador ordinário criou as gratificações de exercício em setores fiscais e os prêmios de produtividade para suprir a lacuna criada com a proibição das participações diretas na arrecadação dos tributos.- Esta fórmula não infringe a norma constitucional recente porque a maneira de atribuir os prêmios não se confunde com a participação na receita, que anteriormente vigorava.- Interpretação do art. 196 da Constituição.
Imposto sobre serviços - Taxatividade da lista e aplicação de seus itens
Authors: Nogueira Ruy Barbosa 
Published: August 2023

Na última reunião semanal da mesa-de-debates do Instituto Brasileiro deDireito Tributário, na Faculdade de Direito da USP, foi proposta a discussão do seguinte tema: "Se o ilustre mestre Aliomar Baleeiro reconhece e declara que a lista de serviços tributáveis pelos municípios é taxativa, como se deverá entender a referência que S. Ex~ faz de que 'cada item da relação comporta interpretação ampla e analógica'?"Como relator da questão, assim expusemos a problemática e as conclusões, que foram aprovadas:
O substituto legal no direito tributário
Authors: Leite Antônio de Oliveira 
Published: August 2023

1. Minha opinião. 2. Caracterização das três figuras: contribuinte,substituto e responsável. 3. Distinção entre contribuinte e substituto.4. Distinção entre substituto e responsável. 5. Confusão entre substitutoe responsável. 6. Necessidade de substituto. 7. O exemplo dassociedades anônimas. 8. Nem sempre é possível a repercussão jurídica.9. Conceito e efeitos da solidariedade tributária. 10. Outro contribuinte: o sujeito passivo, alterativo ou paralelo. 11. O primeiro responsável: o fiador. 12. Traço distintivo ou responsável: posterior contribuinte. Outros responsáveis: os sucessores. 13. Conclusão: necessidade de inclusão de uma nova alínea, e de aditamento em outra alínea, ambas do parágrcijo único do art. 121, do CTN.
Retroatividade do ato administrativo
Authors: Cretella Júnior José 
Published: August 2023

1. Distinções preliminares. 2. Eficácia do ato administrativo. 3. Retroatividade simples. 4. Ato administrativo intertemporal. 5. Autotutela. 6. Direitos adquiridos. 7. Irretroatividade da revogação. 8. Retroatividade da anulação. 9. O regulamento. 10. Conclusão.
Terras ocupadas pelo silvícolas - Posse e domínio - Parque Nacional do Xingu
Authors: Cretella Júnior José 
Published: August 2023

O art. 198 e seus parágrafos da Emenda Constitucional nº 1, de 1969, não tem aplicação retroativa, tendo ressalvado o direito adquirido dos legítimos proprietários, que adquiriram o domínio, mediante ato jurídico perfeito.
Presença norte-americana no direito administrativo brasileiro
Authors: Tácito Caio 
Published: August 2023

1. A influência francesa. 2. As obras pioneiras. 3. Os autores norteamericanos. 4. A regulamentação dos serviços de utilidade pública.5. Atividades econômicas da administração. 6. A renovação promovidapelo DASP. 7. Conclusão.
O contencioso administrativo
Authors: Guimarães Carlos da Rocha 
Published: August 2023

1. Antecedentes históricos. 2. Justiça administrativa. 3. Atos e contratosadministrativos. 4. Questões de direito tributário. 5. Direito previdenciário e acidentes de trabalho. 6. Opção entre justiça administrativa e tribunais judiciários especializados. 7. Conclusão.
O edital nas licitações
Authors: Mello Celso Antônio Bandeira de 
Published: August 2023

1. Introdução. 2. Os princípios da licitação. 3. A licitação comoprocedimento administrativo. 4. Edital. 5. Publicidade do edital. 6.Identificação do objeto licitado e delimitação do universo das propostas.7. Circunscrição do universo de proponentes. 8. Critérios para análise e avaliação de proponentes e propostas. 9. Fixação dos atos e termos do procedimento. 10. Fixação de cláusulas do contrato. 11. Vícios do edital.