bluuebunny/crossref_metadata_2025_split huggingface dataset:
The effect of the anti-leukemia inhibitory factor on the immune system in the Balb/c mice bearing breast cancer induced with 4T1 cells
Authors: Yavari Abolfazl , Zare Fateme , Hadinedoushan Hossein , Tahoori Mohammad Taher 
Published: July 2023

Abstract
Background
Breast cancer is one of the most common cancers. Leukemia inhibitory factor (LIF) is considered as one of the effective factors in the growth of breast cancer, and anti-leukemia inhibitory factor antibody is considered as one of the treatment options for this type of cancer.

Methods
Mice models of breast cancer were made with 4T1 cell line and were randomly divided into four groups. The first group included the mice that received anti-LIF (Anti LIF group). The mice in the second group received anti-LIF and doxorubicin (Anti LIF & DOX). The mice in the third group received only doxorubicin (DOX). Finally, the mice in the fourth group did not receive any intervention. 22 days after tumor induction, some of the mice were killed, and their tumor tissues, lymph nodes, and spleens were separated for evaluating P53, Caspase-3, TIM-3, LAG-3, CTLA-4, and PD-1 genes expression. The percentage of regulatory T cells and level of interferon gamma (IFN-γ) and transforming growth factor-beta (TGF-β) were evaluated. The rest of the mice were kept to check the tumor size and their survival rate.

Results
The proposed intervention did not have any significant effect on the tumor growth and the survival rate. However, the expression of P53 gene and Caspase-3 in the tumor tissue of the Anti LIF group had a significant enhancement. In tumor tissues and lymph nodes, the expression of T-bet, PD-1, TIM-3, and LAG-3 genes in the Anti LIF group showed a significant increase. There was no significant difference between groups in the percentage of regulatory T cells and level of IFN-γ and TGF-β.

Conclusions
The proposed interventions were able to have a direct effect on tumors, but no significant effect was observed on the immune system.
Dietary supplemental coated essential oils and organic acids mixture improves growth performance and gut health along with reduces Salmonella load of broiler chickens infected with Salmonella Enteritidis
Authors: Hu Zeqiong , Liu Lin , Guo Fangshen , Huang Jia , Qiao Jianing , Bi Ruichen , Huang Jinyu , Zhang Kaichen , Guo Yuming , Wang Zhong 
Published: July 2023

Abstract
Background
Reducing Salmonella infection in broiler chickens by using effective and safe alternatives to antibiotics is vital to provide safer poultry meat and minimize the emergence of drug-resistant Salmonella and the spread of salmonellosis to humans. This study was to first evaluate the protective efficacy of feeding coated essential oils and organic acids mixture (EOA) on broiler chickens infected with Salmonella Enteritidis (S. Enteritidis, SE), and then its action mechanism was further explored.

Methods
A total of 480 1-day-old Arbor Acres male chickens were randomly assigned into five treatments with six replicates, including non-challenged control fed with basal diet (A), SE-challenged control (B), and SE-infected birds fed a basal diet with 300 mg/kg of EOA (BL), 500 mg/kg of EOA (BM) and 800 mg/kg of EOA (BH), respectively. All birds on challenged groups were infected with Salmonella Enteritidis on d 13.

Results
Feeding EOA showed a reversed ability on negative effects caused by SE infection, as evidenced by decreasing the feed conversion rate (FCR) and the ratio of villus height to crypt depth (VH/CD) (P < 0.05), obviously decreasing intestinal and internal organs Salmonella load along with increasing cecal butyric acid-producing bacteria abundance (P < 0.05). Moreover, supplemental different levels of EOA notably up-regulated claudin-1 (CLDN-1), occludin (OCLN), zonula occludens-1 (ZO-1), mucin-2 (MUC-2), fatty acid binding protein-2 (FABP-2), nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB), myeloid differential protein-88 (MyD88) and interleukin-6 (IL-6) mRNA levels in the ileum of the infected chickens after challenge, whereas down-regulated toll-like receptor-4 (TLR-4) mRNA levels (P < 0.05). Linear discriminant analysis combined effect size measurements analysis (LEfSe) showed that the relative abundance of g_Butyricicoccus, g_Anaerotruncus and g_unclassified_f_Bacillaceae significantly was enriched in infected birds given EOA. Also, phylogenetic investigation of communities by reconstruction of unobserved states (PICRUSt) analysis showed that alpha-linolenic acid metabolism, fatty acid metabolism and biosynthesis of unsaturated fatty acids were significantly enriched in the EOA group.

Conclusion
Our data suggest that the essential oils and organic acids mixture can be used as an effective strategy to ameliorate and alleviate Salmonella Enteritidis infection in broilers.
Low bone mineral density is found in low weight female youth with avoidant/restrictive food intake disorder and associated with higher PYY levels
Authors: Sella Aluma Chovel , Becker Kendra R. , Slattery Meghan , Hauser Kristine , Asanza Elisa , Stern Casey , Kuhnle Megan , Micali Nadia , Eddy Kamryn T. , Misra Madhusmita , Thomas Jennifer J. , Lawson Elizabeth A. 
Published: July 2023

Abstract
Background
Avoidant/restrictive food intake disorder (ARFID) is a restrictive eating disorder commonly associated with medical complications of undernutrition and low weight. In adolescence, a critical time for bone accrual, the impact of ARFID on bone health is uncertain. We aimed to study bone health in low-weight females with ARFID, as well as the association between peptide YY (PYY), an anorexigenic hormone with a role in regulation of bone metabolism, and bone mineral density (BMD) in these individuals. We hypothesized that BMD would be lower in low-weight females with ARFID than healthy controls (HC), and that PYY levels would be negatively associated with BMD.

Methods
We performed a cross-sectional study in 14 adolescent low-weight females with ARFID and 20 HC 10–23 years old. We assessed BMD (total body, total body less head and lumbar spine) using dual x-ray absorptiometry (DXA) and assessed fasting total PYY concentration in blood.

Results
Total body BMD Z-scores were significantly lower in ARFID than in HC (− 1.41 ± 0.28 vs. − 0.50 ± 0.25, p = 0.021). Mean PYY levels trended higher in ARFID vs. HC (98.18 ± 13.55 pg/ml vs. 71.40 ± 5.61 pg/ml, p = 0.055). In multivariate analysis within the ARFID group, PYY was negatively associated with lumbar BMD adjusted for age (β = -0.481, p = 0.032).

Conclusion
Our findings suggest that female adolescents with low-weight ARFID may have lower BMD than healthy controls and that higher PYY levels may be associated with lower BMD at some, but not all, sites in ARFID. Further research with larger samples will be important to investigate whether high PYY drives bone loss in ARFID.
Study protocol for a randomised controlled trial investigating the effects of Mindfulness Based Stress Reduction on stress regulation and associated neurocognitive mechanisms in stressed university students: the MindRest study
Authors: Kogias Nikos , Geurts Dirk E. M. , Krause Florian , Speckens Anne E. M. , Hermans Erno J. 
Published: July 2023

Abstract
Background
Stress-related disorders are a growing public health concern. While stress is a natural and adaptive process, chronic exposure to stressors can lead to dysregulation and take a cumulative toll on physical and mental well-being. One approach to coping with stress and building resilience is through Mindfulness-Based Stress Reduction (MBSR). By understanding the neural mechanisms of MBSR, we can gain insight into how it reduces stress and what drives individual differences in treatment outcomes. This study aims to establish the clinical effects of MBSR on stress regulation in a population that is susceptible to develop stress-related disorders (i.e., university students with mild to high self-reported stress), to assess the role of large-scale brain networks in stress regulation changes induced by MBSR, and to identify who may benefit most from MBSR.

Methods
This study is a longitudinal two-arm randomised, wait-list controlled trial to investigate the effects of MBSR on a preselected, Dutch university student population with elevated stress levels. Clinical symptoms are measured at baseline, post-treatment, and three months after training. Our primary clinical symptom is perceived stress, with additional measures of depressive and anxiety symptoms, alcohol use, stress resilience, positive mental health, and stress reactivity in daily life. We investigate the effects of MBSR on stress regulation in terms of behaviour, self-report measures, physiology, and brain activity. Repetitive negative thinking, cognitive reactivity, emotional allowance, mindfulness skills, and self-compassion will be tested as potential mediating factors for the clinical effects of MBSR. Childhood trauma, personality traits and baseline brain activity patterns will be tested as potential moderators of the clinical outcomes.

Discussion
This study aims to provide valuable insights into the effectiveness of MBSR in reducing stress-related symptoms in a susceptible student population and crucially, to investigate its effects on stress regulation, and to identify who may benefit most from the intervention.

Trial registration
Registered on September 15, 2022, at clinicaltrials.gov, NCT05541263.
Bibliometric mining of research directions and trends for big data
Authors: Lundberg Lars 
Published: July 2023

AbstractIn this paper a program and methodology for bibliometric mining of research trends and directions is presented. The method is applied to the research area Big Data for the time period 2012 to 2022, using the Scopus database. It turns out that the 10 most important research directions in Big Data are Machine learning, Deep learning and neural networks, Internet of things, Data mining, Cloud computing, Artificial intelligence, Healthcare, Security and privacy, Review, and Manufacturing. The role of Big Data research in different fields of science and technology is also analysed. For four geographic regions (North America, European Union, China, and The Rest of the World) different activity levels in Big Data during different parts of the time period are analysed. North America was the most active region during the first part of the time period. During the last years China is the most active region. The citation scores for documents from different regions and from different research directions within Big Data are also compared. North America has the highest average citation score among the geographic regions and the research direction Review has the highest average citation score among the research directions. The program and methodology for bibliometric mining developed in this study can be used also for other large research areas. Now that the program and methodology have been developed, it is expected that one could perform a similar study in some other research area in a couple of days.
Optical changes and association with axial elongation in children wearing orthokeratology lenses of different back optic zone diameter
Authors: Guo Biyue , Cho Pauline , Cheung Sin Wan , Kojima Randy , Vincent Stephen 
Published: July 2023

Abstract
Purpose
To compare changes in ocular aberrations in children wearing orthokeratology (ortho-k) lenses with a back optic zone diameter (BOZD) of 6 mm (6-MM group) or 5 mm (5-MM group) and their associations with axial elongation (AE) over two years.

Methods
Seventy Chinese children, aged 6 to < 11 years, with myopia between − 4.00 to − 0.75 D, were randomly allocated to 5-MM and 6-MM groups. Ocular aberrations were measured, rescaled to a 4-mm pupil, and fitted with a 6th order Zernike expansion. Measurements, including axial length, were taken prior to commencing ortho-k treatment and then every six months over two years.

Results
After two years, the 5-MM group displayed a smaller horizontal treatment zone (TZ) diameter (by 1.14 ± 0.11 mm, P < 0.001) and less AE (by 0.22 ± 0.07 mm, P = 0.002) compared with the 6-MM group. A greater increase in total root mean square (RMS) of higher-order aberrations (HOAs), primary spherical aberration (SA) ($${\mathrm{C}}_{4}^{0})$$

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, and coma were also observed in the 5-MM group at all follow-up visits. The horizontal TZ diameter was significantly associated with changes in RMS HOAs, SA (RMS, primary and secondary SA), and RMS coma. After controlling for baseline parameters, RMS HOAs, RMS SA, RMS coma, and primary ($${\mathrm{C}}_{4}^{0})$$

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and secondary ($${\mathrm{C}}_{6}^{0})$$

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SA were significantly associated with AE.

Conclusions
Ortho-k lenses with a smaller BOZD created a smaller horizontal TZ diameter and a significant increase in total HOAs, total SA, total coma, and primary SA and a decrease in secondary SA. Of these ocular aberrations, total HOAs, total SA, and primary SA were negatively correlated with AE over two years.
Trial registration: ClinicalTrial.gov, NCT03191942. Registered 19 June 2017, https://clinicaltrials.gov/ct2/show/NCT03191942.
Incidence and characteristics of aqueous misdirection after glaucoma surgery in Chinese patients with primary angle-closure glaucoma
Authors: Lin Haishuang , Li Jiaqian , Zheng Xuanli , Wan Rui , Zhou Mengtian , Ding Yutong , Ji Yiting , Xie Yanqian , Tham Clement C. , Zhang Shaodan , Liang Yuanbo 
Published: July 2023

Abstract
Background
To report the incidence and clinical characteristics of aqueous misdirection (AM) after glaucoma surgery in Chinese patients with primary angle-closure glaucoma.

Methods
Medical records of all patients diagnosed with primary angle-closure glaucoma who underwent glaucoma surgery in the Eye Hospital of Wenzhou Medical University between January 2012 and December 2021 were retrospectively reviewed. Cases of AM were identified through a keyword-based search. The incidence of AM was calculated. Demographic and clinical characteristics of the AM patients were also described.

Results
A total of 5044 eyes with primary angle-closure glaucoma were included (mean age 65.81 ± 9.96 years, 68.11% women). Thirty-eight eyes developed AM, presenting an overall incidence of 0.75%. The mean time interval between surgery and first record of AM diagnosis was 2.57 ± 5.24 months (range, 0 day to 24 months). The incidence of AM was significantly higher in patients aged ≤ 40 years (21.28%) and those aged 40–50 years (3.32%), compared to those > 50 years (0.42%) (P < 0.001). AM developed much more frequently among patients with chronic angle-closure glaucoma (1.30%), compared to those with acute angle-closure glaucoma (0.32%, P < 0.001). Eleven eyes (0.37%) developed AM following non-filtering surgery compared to 24 eyes (2.27%) after filtering surgery (P < 0.001).

Conclusion
The incidence of AM after glaucoma surgery was 0.75% in Chinese patients with primary angle closure glaucoma. Younger age, chronic angle-closure glaucoma, and undergoing filtering surgery, were identified as associated risk factors for developing AM. Phacoemulsification may have less risk of developing AM compared to filtering surgery.
TP53 mutations in Romanian patients with colorectal cancer
Authors: Manirakiza Felix , Yamada Hidetaka , Iwashita Yuji , Ishino Keiko , Ishikawa Rei , Kovacs Zsolt , Osvath Eva , Nzitakera Augustin , Gurzu Simona , Sugimura Haruhiko 
Published: July 2023

Abstract
Background
Colorectal cancer (CRC) has been ranked as the second most deadly cancer and the third most diagnosed cancer cases for the year 2020. Specifically for Romania, the number of CRC-related deaths in 2019 was estimated at 6307 people, with a standardized mortality rate of 33.8 per 100,000 inhabitants. Although the tumor protein 53 (TP53) gene is intensively studied, there are few data on TP53 mutations in Romanian CRC. Furthermore, since genetic alterations may show geographical differences, our study aimed to analyze the clinical status and TP53 somatic variation in Romanian CRC patients.

Subjects and methods
DNA from 40 randomly selected cases of CRC was extracted from formalin-fixed paraffin-embedded tissues and sequenced using direct Sanger sequencing techniques, and variants were annotated according to the recommendations of the Human Genome Variation Society. Novel variants were analyzed using MutationTaster2021 to predict their effects.

Results
The mean age was 63.6 years (range 33–85 years) with a male to female ratio of 2.3. More than 45% (18/40) had an advanced cancer stage (≥ stage III). Mutations were found in 21/40 cases (52.5%), with one case having two mutations, giving a total of twenty-two mutations in the TP53 coding DNA. These mutations include 3 (13.6%) insertion-deletion mutations, two of which are novel frameshift mutations: c.165delT (in exon 4) and c.928_935dup (in exon 9), both of which are predicted to lead to nonsense-mediated mRNA decay and are classified as deleterious. The remaining 19 (86.36%) were substitution mutations: 1 nonsense and 18 (81.8%) missense mutations, with G > A (n = 7/19; 36.8%) and C > T (n = 6/19; 31.5%) transitions being the most common. The G > T transversion was found in 21.05% (4/19) of the substitution mutations.

Conclusion
We have described two novel frameshift mutations in TP53. The discovery of novel mutations following the efforts of The Cancer Genome Atlas and other large-scale cancer genome sequencing projects may be further evidence of the heterogeneous nature of mutations in cancer and may indicate that the identification of carcinogenic mutations is not yet saturated. Further sequencing is therefore needed, especially in less studied populations. Importantly, consideration of their geographical environment will shed light on population-specific carcinogenesis.
SkillSim: voice apps similarity detection
Authors: Guo Zhixiu , Liang Ruigang , Meng Guozhu , Chen Kai 
Published: July 2023

AbstractVirtual personal assistants (VPAs), such as Amazon Alexa and Google Assistant, are software agents designed to perform tasks or provide services to individuals in response to user commands. VPAs extend their functions through third-party voice apps, thereby attracting more users to use VPA-equipped products. Previous studies demonstrate vulnerabilities in the certification, installation, and usage of these third-party voice apps. However, these studies focus on individual apps. To the best of our knowledge, there is no prior research that explores the correlations among voice apps.Voice apps represent a new type of applications that interact with users mainly through a voice user interface instead of a graphical user interface, requiring a distinct approach to analysis. In this study, we present a novel voice app similarity analysis approach to analyze voice apps in the market from a new perspective. Our approach, called SkillSim, detects similarities among voice apps (i.e. skills) based on two dimensions: text similarity and structure similarity. SkillSim measures 30,000 voice apps in the Amazon skill market and reveals that more than 25.9% have at least one other skill with a text similarity greater than 70%. Our analysis identifies several factors that contribute to a high number of similar skills, including the assistant development platforms and their limited templates. Additionally, we observe interesting phenomena, such as developers or platforms creating multiple similar skills with different accounts for purposes such as advertising. Furthermore, we also find that some assistant development platforms develop multiple similar but non-compliant skills, such as requesting user privacy in a non-compliance way, which poses a security risk. Based on the similarity analysis results, we have a deeper understanding of voice apps in the mainstream market.
Parametric study on prestressed skewed box-girder bridge
Authors: Agarwal Preeti , Singh Deepak Kumar 
Published: July 2023

AbstractThis study employs the finite element-based CSiBridge v.20.0.0 software to examine the response of a single-cell prestressed box-girder bridge subjected to Indian loading conditions. The analyses is carried out on a simply supported bridge considering the specifications of Indian Road Congress (IRC) 6:2017, IRC 18:2000 and IRC 21:2000. An existing model of prestressed skewed bridge is validated with the published one. A convergence study is conducted for determining the model’s mesh size. An extensive parametric study is carried out to gain a better understanding of the response of a skewed prestressed bridge. The parameters variables are: Skew angle (0°, 10°, 20°, 30°, 40°, 50°, and 60°); Span (35, 40, 45, 50, 55, and 60 m); and Span-depth ratio (10, 12, 14, 16 and 18). The results of this study are presented as ratios of Bending moment, Shear force, Torsional moment, and Vertical deflection. Finally, equations for estimation of these ratios for different span and span-depth ratio are also deduced from the statistical approach so that the results of skewed bridges may be evaluated directly. It is determined that the skewed bridge outperforms the straight bridge because of its higher span-depth ratio, which results in less bending moment development. Evidence suggests that the skewness may help to lessen the prestress load’s dominance. The findings of this study may be helpful to engineers and designers in the analysis and design of prestresssed skewed box-girder bridges.
Vigorously cited: a bibliometric analysis of the 100 most cited sedentary behaviour articles
Authors: Memon Aamir R. , Chen Sitong , To Quyen G. , Vandelanotte Corneel 
Published: July 2023

Abstract
Objectives
No citation analysis has examined peer-reviewed sedentary behaviour research articles, which is needed to assess the impact of this research and identify knowledge gaps. Therefore, this study aimed to identify the 100 most cited sedentary behaviour articles and examine their bibliometric characteristics.

Methods
All databases indexed in the Web of Science database were searched in October 2022, and bibliometric characteristics of the studies, irrespective of the publication year, were imported and calculated. Descriptive statistics and visualisations by the VOSviewer were used for the presentation of bibliometric characteristics.

Results
The 100 most cited articles received 49,062 citations in total, with a median citation density of 32.5 citations per article per year. The majority of included articles were reviews (n = 48; 22,856 citations), focused on adults (58%; 26,809 citations) and reported on the relationship of sedentary behaviour with health (n = 64; 34,598 citations); more specifically they focused on anthropometric indices (28%), metabolic health (24%), and mortality (23%). The United States was ranked first in terms of the overall for most cited articles. However, Australia was ranked first for institutions and authors contributing to the most cited sedentary behaviour articles.

Conclusions
Papers published after 2007 were predominant in the list of 100 most cited sedentary behaviour papers, as were those focusing on associations with physical health outcomes and those focusing on adults. While original articles were cited more, discussion papers had more impact on the field as they received more citations in less time. Research examining associations between sedentary behaviour and health was cited more. The field is dominated by contributions from high-income countries.