bluuebunny/crossref_metadata_2025_split huggingface dataset:
Genetic circuits for feedback control of gamma-aminobutyric acid biosynthesis in probioticEscherichia coliNissle 1917
Authors: Lebovich Matthew , Andrews Lauren B. 
Published: June 2023

AbstractEngineered microorganisms such as the probiotic strainEscherichia coliNissle 1917 (EcN) offer a strategy to sense and modulate the concentration of metabolites or therapeutics in the gastrointestinal tract. Here, we present an approach to regulate production of the depression-associated metabolite gamma-aminobutyric acid (GABA) in EcN using genetic circuits that implement negative feedback. We engineered EcN to produce GABA by overexpressing glutamate decarboxylase (GadB) fromE. coliand applied an intracellular GABA biosensor to identify growth conditions that improve GABA biosynthesis. We next employed characterized genetically-encoded NOT gates to construct genetic circuits with layered feedback to control the rate of GABA biosynthesis and the concentration of GABA produced. Looking ahead, this approach may be utilized to design feedback control of microbial metabolite biosynthesis to achieve designable smart microbes that act as living therapeutics.
Pontiella agarivoranssp. nov., a novel marine anaerobic bacterium capable of degrading macroalgal polysaccharides and fixing nitrogen
Authors: Liu Na , Kivenson Veronika , Peng Xuefeng , Cui Zhisong , Lankiewicz Thomas S. , Gosselin Kelsey M. , O’Malley Michelle A. , Valentine David L. 
Published: June 2023

AbstractMarine macroalgae produce abundant and diverse polysaccharides which contribute substantially to the organic matter exported to the deep ocean. Microbial degradation of these polysaccharides plays an important role in the turnover of macroalgal biomass. Various members of thePlanctomycetes-Verrucomicrobia-Chlamydia(PVC) superphylum are degraders of recalcitrant polysaccharides in widespread anoxic environments. In this study, we isolated a novel anaerobic bacterial strain NLcol2Tfrom microbial mats on the surface of marine sediments offshore Santa Barbara, California, USA. Based on 16S rRNA gene and phylogenomic analyses, strain NLcol2Trepresents a novel species within thePontiellagenus in theKiritimaitellaeotaphylum (within the PVC superphylum). Strain NLcol2Tis able to utilize various monosaccharides, disaccharides, and macroalgal polysaccharides such as agar and iota-carrageenan. A near-complete genome also revealed an extensive metabolic capacity for anaerobic degradation of sulfated polysaccharides, as evidenced by 202 carbohydrate-active enzymes (CAZymes) and 165 sulfatases. Additionally, its ability of nitrogen fixation was confirmed by nitrogenase activity detected during growth on nitrogen-free medium, and the presence of nitrogenases (nifDKH) encoded in the genome. Based on the physiological and genomic analyses, this strain represents a group of bacteria that may play an important role in the degradation of macroalgal polysaccharides and with relevance to the biogeochemical cycling of carbon, sulfur, and nitrogen in marine environments. Strain NLcol2T(= DSM 113125T= MCCC 1K08672) is proposed to be the type strain of a novel species inPontiellagenus, and the namePontiella agarivoranssp. nov. is proposed.ImportanceThe growth and preservation of marine macroalgae is considered as a carbon dioxide reduction strategy. However, we still have limited knowledge about the eventual fate of carbon stored in macroalgae. Diverse heterotrophic microbial communities in the ocean specialize on these complex polymers, for example, members in theKiritimatiellaeotaphylum. However, only four type strains within the phylum have been cultured and characterized to date, and there is limited knowledge about the metabolic capabilities and functional role of this phylum in the environment. The new isolate strain NLcol2Texpands the known substrate range of this phylum with the capability to utilize macroalgal polysaccharides agar and iota-carrageenan. It is also the first strain in theKiritimatiellaeotaphylum to be reported with nitrogen fixing ability.
Travelling waves due to negative plant-soil feedbacks in a model including tree life-stages
Authors: Iuorio Annalisa , Baudena Mara , Eppinga Maarten B. , Giannino Francesco , Rietkerk Max , Veerman Frits 
Published: June 2023

AbstractThe emergence and maintenance of tree species diversity in tropical forests is commonly attributed to the Janzen-Connell (JC) hypothesis, which states that growth of seedlings is suppressed in the proximity of conspecific adult trees. As a result, a JC distribution due to a density-dependent negative feedback emerges in the form of a (transient) pattern where conspecific seedling density is highest at intermediate distances away from parent trees. Several studies suggest that the required density-dependent feedbacks behind this pattern could result from interactions between trees and soil-borne pathogens. However, negative plant-soil feedback may involve additional mechanisms, including the accumulation of autotoxic compounds generated through tree litter decomposition. An essential task therefore consists in constructing mathematical models incorporating both effects showing the ability to support the emergence of JC distributions.In this work, we develop and analyse a novel reaction-diffusion-ODE model, describing the interactions within tropical tree species across different life stages (seeds, seedlings, and adults) as driven by negative plant-soil feedback. In particular, we show that under strong negative plant-soil feedback travelling wave solutions exist, creating transient distributions of adult trees and seedlings that are in agreement with the Janzen-Connell hypothesis. Moreover, we show that these travelling wave solutions are pulled fronts and a robust feature as they occur over a broad parameter range. Finally, we calculate their linear spreading speed and show its (in)dependence on relevant nondimensional parameters.2020 MSC35C07, 34C60, 34D05, 35K57, 37C25, 65M06, 92D40.
Allelic chromatin structure primes imprinted expression ofKcnk9during neurogenesis
Authors: Loftus Daniel , Bae Bongmin , Whilden Courtney M. , Whipple Amanda J. 
Published: June 2023

AbstractDifferences in chromatin state inherited from the parental gametes influence the regulation of maternal and paternal alleles in offspring. This phenomenon, known as genomic imprinting, results in genes preferentially transcribed from one parental allele. While local epigenetic factors such as DNA methylation are known to be important for the establishment of imprinted gene expression, less is known about the mechanisms by which differentially methylated regions (DMRs) lead to differences in allelic expression across broad stretches of chromatin. Allele-specific higher-order chromatin structure has been observed at multiple imprinted loci, consistent with the observation of allelic binding of the chromatin-organizing factor CTCF at multiple DMRs. However, whether allelic chromatin structure impacts allelic gene expression is not known for most imprinted loci. Here we characterize the mechanisms underlying brain-specific imprinted expression of thePeg13-Kcnk9locus, an imprinted region associated with intellectual disability. We performed region capture Hi-C on mouse brain from reciprocal hybrid crosses and found imprinted higher-order chromatin structure caused by the allelic binding of CTCF to thePeg13DMR. Using anin vitroneuron differentiation system, we show that on the maternal allele enhancer-promoter contacts formed early in development prime the brain-specific potassium leak channelKcnk9for maternal expression prior to neurogenesis. In contrast, these enhancer-promoter contacts are blocked by CTCF on the paternal allele, preventing paternalKcnk9activation. This work provides a high-resolution map of imprinted chromatin structure and demonstrates that chromatin state established in early development can promote imprinted expression upon differentiation.
Mediation approach of knowledge management process towards supply chain performance under green technology: an empirical analysis
Authors: Alam Sajjad , Zhang Jianhua , Shehzad Muhammad Usman , Khan Naveed , Ali Ahmad 
Published: June 2023

PurposeEmployee knowledge is an essential contributor to managing supply chain ambiguities and supply improvement. The current research examined green technology (GT) implementation and knowledge management (KM) processes toward knowledge worker supply performance (KWSP). The present study postulates that GT implementation's positive impacts on the KM process and KM process lead to improved KWSP based on employee knowledge contribution.Design/methodology/approachTo accomplish the research objective, the data were taken from those production houses that are utilizing GT. A total of 400 questionnaires were distributed in Zhengzhou city, and feedback was received (343). The feedback data were studied through partial least squares-structural equation modelling (PLS-SEM) and fuzzy set qualitative comparative analysis (fsQCA). fsQCA is used to analyze improving the multiple configurational paths of KWSP.FindingsThe study revealed that GT implementation is positively associated with the KM process, and KM processes substantially influence the improvement of KWSP. The consequence of fsQCA demonstrated that various combinations improve supply chain performance. The study combines certain dimensions of GT implementation with the KM process, revealing that supply chain performance improved based on knowledge sharing in manufacturing firms.Research limitations/implicationsThis study guides decision-makers and academics on using the knowledge process associated with GT to improve supply chain performance. Moreover, future researchers should use information technology in supply houses and compare the result with the literature to generalize the results better.Originality/valueThe asymmetric technique assists in differentiating the connections that are not directly investigated by the traditional symmetric method. The study covers and identifies various approaches that can be used in manufacturing firms to improve the supply chain process and employee satisfaction. The study combines the two techniques to understand supply performance based on knowledge sharing in manufacturing firms.
Understanding BMI from Industry 4.0, knowledge management and market uncertainty perspective
Authors: Kafetzopoulos Dimitrios 
Published: June 2023

PurposeThe main purpose of this study is to investigate the role of Industry 4.0 capability (I4.0 C) in creating a supportive environment for business model innovation by focussing on the mediating role of knowledge management capability (KMC) and the moderating effect of market uncertainty.Design/methodology/approachThis paper uses interviews with managers and academics. Data were collected from 379 managers; CFA and finally regression analysis were performed, and the program “PROCESS” software was used to validate the data and examine the hypothesized relationships.FindingsKMC mediates the relationship between Industry 4.0 capability and business model innovation. The effect of Industry 4.0 capability on KMC is stronger for firms with increased market uncertainty. In contrast, the impact of KMC on business model innovation is weaker for firms operating in highly-uncertain markets.Research limitations/implicationsData were collected at only one point in time from one country, Greece. This might pose limitations on the generalizability of our results. Future research should test the relationships examined in this study in other international contexts.Practical implicationsManagers should invest in more advanced technology in order to obtain knowledge and capability. In addition, they need to pay more attention to how their firms' knowledge derived from Industry 4.0 contributes to business model innovation.Originality/valueThe current state of knowledge of both theory and practise for critical organizational factors such as Industry 4.0 capability, KMC, business model innovation and market uncertainty will be extended.
Work automation and the rise of virtual teams: how to lead employees in the post-pandemic world
Authors: Murphy Liam 
Published: June 2023

Purpose
To support senior leaders and HR practitioners with building the internal leadership capabilities to oversee automation in a virtual teams environment.

Design/methodology/approach
This point of view paper presents the topic of workplace automation in a virtual teams environment through contextual practitioner literature sources.

Findings
Six new capabilities are suggested for the modern leader.

Originality/value
This paper introduces a scarcely researched area which is of enormous relevance in the post-covid age of remote working and digital transformation agendas, alongside presenting recommendations for HR practitioners and senior leaders to build internal leadership capabilities.
A bitter pill to swallow: the model of despotic leadership, bullying behavior, emotional intelligence and well-being
Authors: Islam Talat , Chaudhary Arooba , Ali Hafiz Fawad 
Published: June 2023

PurposeThis study aims to investigate how despotic leadership affects employee well-being through bullying behavior. The study further investigates emotional intelligence as a conditional variable on the association between bullying behavior and employee well-being.Design/methodology/approachThe data from 257 nurses and their immediate supervisors (dyads) were collected on convenience basis using a cross-sectional design. Further, structural equation modeling was used to analyze the data.FindingsThe study noted that despotic leadership negatively affects employee well-being. Specifically, despotic leaders were noted to trigger employees’ bullying behavior that ultimately diminish their well-being. The study noted emotional intelligence as a conditional variable such that individuals with high emotional intelligence are more likely to buffer the negative association between bullying behavior and employee well-being.Research limitations/implicationsThis study highlights the importance of employee well-being and suggests the management focus on their leadership style. Further, the study suggests to Human Resource practitioners the importance of personality traits (emotional intelligence) at the time of recruitment, as it serves as a coping strategy to diminish employee well-being.Originality/valueDrawing upon the conservation of resources, this study shed light on the mediating role of bullying behavior between negative leadership (despotic) and well-being. In addition, emotional intelligence has not been examined as a conditional variable between bullying behavior and employee well-being.
Celebrate with me: a black adolescent girl’s speculative multimodal design of intersectional college and career futures
Authors: Turner Jennifer D. 
Published: June 2023

Purpose
This paper aims to demonstrate how Alayah, a 16-year-old African American girl, leverages multiple expressive modes for intersectional self-representation as speculative design. Here, speculative design refers to a multimodal composition (i.e. digital collage) which leverages multiple expressive modes for intersectional self-celebration in possible futures.

Design/methodology/approach
Informed by intersectional multimodal literacy frameworks and analyses, this paper addresses the question, “How does Alayah represent her college and career futures in her speculative multimodal design? To address this question, the author analyzed Alayah's digital collage using an intersectional multimodal analysis template complemented by a thematic analysis of her interview data and the narrated explanation of her collage.

Findings
In a speculative design composed of 15 images and words, Alayah agentively determined and critically celebrated her intersectional college and career futures through four interrelated themes: Black girl affirmation; Collegiate success; “Sweet” work; and Black livingness.

Originality/value
By centering Black girls’ speculative multimodal designs in college and career curricula, ELA educators (re)imagine college and career pedagogies to critically celebrate Black adolescent girls as intelligent, empowered and literate young women worthy of the futures that they desire.
Social sustainability of continuing care retirement communities in China
Authors: Hu Xin 
Published: June 2023

Purpose
China’s population is ageing. Continuing care retirement communities (CCRCs) are an emerging living arrangement of older Chinese. Incorporating social sustainability features into CCRCs helps to create age-friendly residential environments for residents. However, it is still unclear what kinds of social sustainability features are incorporated into the residential environments of CCRCs in China. Therefore, this study aims to address this research gap.

Design/methodology/approach
Qualitative content analysis is adopted to analyse the retrieved business information of representative CCRC developers in China.

Findings
This study revealed 36 social sustainability features in CCRCs, with the top-ranked ones being health care and management, social connection and engagement, high-quality and diverse services and daily life support and assistance. Additionally, a preliminary social sustainability framework of CCRCs was proposed, and this framework includes the five components of care and health, environment and management, service and facility, age-friendly life philosophy and social support and inclusion.

Originality/value
In theory, this research’s findings clarify the meaning of social sustainability within the context of CCRCs, which supports future relevant explorations in the CCRC research community. In practice, these findings enhance stakeholders’ understanding of the social sustainability in CCRCs, which promotes the development of age-friendly living environments for older people in an ageing society.
Covid-19 crisis management human resource cost-retrenchment: the role of transformational leadership and ethical climate
Authors: Shulga Lenna V. , Busser James A. 
Published: June 2023

Purpose
As the tourism industry emerges from full or partial closure caused by the COVID-19 crisis, it is imperative to understand the internal conditions that assisted organizations to maintain positive employee attitudes despite the adverse effects of unpopular cost–retrenchment strategies. Therefore, this study aims to understand the impacts of transformational leadership (TFL), human resource management (HRM) crisis cost–retrenchment and ethical climate (EC) on employee job outcomes affected by COVID-19 pandemic.

Design/methodology/approach
Mid-level managers of service organizations from a travel destination heavily reliant on the tourism participated in an online self-administered survey one month after the state eased its COVID-19 travel restrictions. Partial least square structural equation modeling (PLS-SEM) examined how TFL and EC influenced cost–retrenchment crisis–management HRM, satisfaction and trust in the organization, followed by PLS multi-group analysis (PLS-MGA) to understand differences between hospitality and non-hospitality employees.

Findings
Results revealed an overall positive effect of TFL that diminished the negative affect of HRM cost-retrenchment on employee satisfaction. PLS-MGA showed a significant positive role of other-focused EC on employee outcomes, especially for hospitality organizations, whereas self-focused EC had a negative impact for non-hospitality firms.

Originality/value
This study contributes to contingency theory of leadership by demonstrating that TFL in combination with EC mitigates or overpowers the negative effects of cost–retrenchment crisis management strategies on employees. The study advances knowledge of self-focused and other-focused moral reasoning climate impacts under COVID-19 conditions for hospitality organizations. The industry comparison results highlight the important positive characteristics of hospitality crisis management.
Letting AI make decisions for me: an empirical examination of hotel guests’ acceptance of technology agency
Authors: Morosan Cristian , Dursun-Cengizci Aslıhan 
Published: June 2023

Purpose
This study aims to examine hotel guests’ acceptance of technology agency – the extent to which they would let artificial intelligence (AI)-based systems make decisions for them when staying in hotels. The examination was conducted through the prism of several antecedents of acceptance of technology agency, including perceived ethics, benefits, risks and convenience orientation.

Design/methodology/approach
A thorough literature review provided the foundation of the structural model, which was tested using confirmatory factor analysis, followed by structural equation modeling. Data were collected from 400 US hotel guests.

Findings
The most important determinant of acceptance of technology agency was perceived ethics, followed by benefits. Risks of using AI-based systems to make decisions for consumers had a negative impact on acceptance of technology agency. In addition, perceived loss of competence and unpredictability had relatively strong impacts on risks.

Research limitations/implications
The results provide a conceptual foundation for research on systems that make decisions for consumers. As AI is increasingly incorporated in the business models of hotel companies to make decisions, ensuring that the decisions are perceived as ethical and beneficial for consumers is critical to increase the utilization of such systems.

Originality/value
Most research on AI in hospitality is either conceptual or focuses on consumers’ intentions to stay in hotels that may be equipped with AI technologies. Occupying a unique position within the literature, this study discusses the first time AI-based systems that make decisions for consumers. The value of this study stems from the examination of the main concept of technology agency, which was never examined in hospitality.
Identifying the drivers responsible for energy security in selected ASEAN countries by using panel data analysis
Authors: Manni Umme Humayara , Hj. Md. Mansur Datuk. Dr. Kasim 
Published: June 2023

Purpose
Energy security has been talked about by governments and policymakers because the global energy market is unstable and greenhouse gas emissions threaten the long-term health of the global environment. One of the most potent ways to cut CO2 emissions is through the production and consumption of renewable energy. Thus, the purpose of this paper is to highlight the drivers that, if ambitious environmental policies are implemented, might improve energy security or prevent its deterioration.

Design/methodology/approach
The study uses a balanced panel data set for Indonesia, Malaysia, the Philippines, Singapore, Thailand and Vietnam that covers a period of 30 years (1990–2020). The pooled panel dynamic least squares is used in this study.

Findings
The findings show that renewable energy consumption is positively related to gross domestic product per capita, energy intensity per capita and renewable energy installed capacity. Wherein renewable energy use is inversely related to per capita electricity consumption, CO2 emissions and the use of fossil fuel electricity.

Originality/value
There is a lack of research identifying the factors influencing energy security in the ASEAN region. Therefore, this study focuses on the drivers that influence energy security, which are explained by the proportion of renewable energy in final energy consumption. Without identifying the demand and supply sources of energy, especially electricity production based on renewable energy techniques, it is hard for policymakers to achieve the desired renewable energy-based outcome.