bluuebunny/crossref_metadata_2025_split huggingface dataset:
Diversity and ecological functions of viruses inhabiting the oil reservoirs
Authors: An Liyun , Liu Xinwu , Wang Jianwei , Xu Jingbo , Chen Xiaoli , Liu Xiaonan , Hu Bingxin , Nie Yong , Wu Xiao-Lei 
Published: September 2023

AbstractOil reservoirs, being one of the most significant subsurface repositories of energy and carbon, have long hosted diverse microorganisms affecting energy production and carbon emissions. Viruses play crucial roles in the ecology of microbiomes, however, their distribution and ecological significance in oil reservoirs remain undetermined. Here, we assembled an extensive catalogue encompassing viral and prokaryotic genomes sourced from oil reservoirs. The catalogue comprises 7,229 prokaryotic genomes and 6,218 viral genomes from 182 oil reservoir metagenomes, respectively. Based on sequence clustering we identified 3,886 Operational Taxonomic Units (vOTUs) approximately at species level, 94.80% of which were not found in any other environments. The results showed that viruses were widely distributed in oil reservoirs, and oil reservoirs contain a significant number of unique and unexplored viruses. We also constructed a catalogue of 322,060 viral gene clusters including 105 virus-encoded putative auxiliary metabolic genes (AMGs) that participate in host metabolism and adaptation to the environment. Furthermore, our investigation has yielded a total of 7,197 putative virus-host pairs based on CRISPR and tRNA profiles. Viruses within oil reservoirs profoundly infected bacterial Actinobacteriota, Desulfobacterota, as well as archaeal Halobacteriota and Methanobacteriota. Combined microcosm enrichment experiments and bioinformatics analysis, we validated the ecological roles of viruses in regulating the community structure of sulfate reduction microorganisms, primarily through the predation of virulent viruses. Collectively, these findings have unveiled a rich diversity of novel viruses and their ecological functions within oil reservoirs. This study provides a comprehensive understanding of the role of viral communities in the biogeochemical cycle of the deep biosphere. It also facilitates microbial applications aimed at addressing challenges related to fossil-fuel production and carbon emissions in the petroleum industry.
Bacterial Efflux Pump Modulators Prevent Bacterial Growth in Macrophages and Under Broth Conditions that Mimic the Host Environment
Authors: Allgood Samual C. , Su Chih-Chia , Crooks Amy L. , Meyer Christian T. , Zhou Bojun , Betterton Meredith D. , Barbachyn Michael R. , Yu Edward W. , Detweiler Corrella S. 
Published: September 2023

ABSTRACTNew approaches for combatting microbial infections are needed. One strategy for disrupting pathogenesis involves developing compounds that interfere with bacterial virulence. A critical molecular determinant of virulence for Gram-negative bacteria are efflux pumps of the resistance-nodulation-division (RND) family, which includes AcrAB-TolC. We previously identified small molecules that bind AcrB, inhibit AcrAB-TolC, and do not appear to damage membranes. These efflux pump modulators (EPMs) were discovered in an in-cell screening platform called SAFIRE (Screen for Anti-infectives using Fluorescence microscopy of IntracellulaR Enterobacteriaceae). SAFIRE identifies compounds that disrupt the growth of a Gram-negative human pathogen,Salmonella entericaserotype Typhimurium (S.Typhimurium) in macrophages. We used medicinal chemistry to iteratively design ∼200 EPM35 analogs and test them for activity in SAFIRE, generating compounds with nanomolar potency. Analogs were demonstrated to bind AcrB in a substrate binding pocket by cryo-electron microscopy (cryo-EM). Despite having amphipathic structures, the EPM analogs do not disrupt membrane voltage, as monitored by FtsZ localization to the cell septum. The EPM analogs had little effect on bacterial growth in standard Mueller Hinton Broth. However, under broth conditions that mimic the micro-environment of the macrophage phagosome,acrABis required for growth, the EPM analogs are bacteriostatic, and increase the potency of antibiotics. These data suggest that under macrophage-like conditions the EPM analogs prevent the export of a toxic bacterial metabolite(s) through AcrAB-TolC. Thus, compounds that bind AcrB could disrupt infection by specifically interfering with the export of bacterial toxic metabolites, host defense factors, and/or antibiotics.IMPORTANCEBacterial efflux pumps are critical for resistance to antibiotics and for virulence. We previously identified small molecules that inhibit efflux pumps (efflux pump modulators, EPMs) and prevent pathogen replication in host cells. Here we used medicinal chemistry to increase the activity of the EPMs against pathogens in cells into the nanomolar range. We show by cryo-electron microscopy that these EPMs bind an efflux pump subunit. In broth culture, the EPMs increase the potency (activity), but not the efficacy (maximum effect), of antibiotics. We also found that bacterial exposure to the EPMs appear to enable the accumulation of a toxic metabolite that would otherwise be exported by efflux pumps. Thus, inhibitors of bacterial efflux pumps could interfere with infection not only by potentiating antibiotics, but also by allowing toxic waste products to accumulate within bacteria, providing an explanation for why efflux pumps are needed for virulence in the absence of antibiotics.
Incomplete immunity in a natural animal-microbiota interaction boosts pathogen virulence
Authors: Hoang Kim L. , Read Timothy D. , King Kayla C. 
Published: September 2023

AbstractStrong partial immunity in recovered hosts is predicted to favour more virulent pathogens upon re-infection in the population. We present empirical evidence that the incomplete immunity generated by commensal host microbiota can similarly select for higher pathogen virulence. We tracked the evolutionary trajectories of a widespread pathogen (Pseudomonas aeruginosa) experimentally passaged through populations of nematode hosts which had been immune-primed by a natural commensal. Immune protection selected for pathogens more than twice as likely to kill the nematode as those evolved in non-primed or immune-compromised animals. Despite the higher virulence that emerged, pathogen molecular evolution in immune-primed hosts was slower and more constrained compared to evolution in immune-compromised hosts, where substantial genetic differentiation was exhibited. These findings directly attribute the partial protective immunity provided by host-microbiome interactions as a significant selective force shaping the virulence and evolutionary dynamics of novel infectious diseases.
Alternative splice variants of the mitochondrial fission proteinDNM1L/Drp1 regulate mitochondrial dynamics and tumor progression in ovarian cancer
Authors: Javed Zaineb , Shin Dong Hui , Pan Weihua , White Sierra R. , Kim Yeon Soo , Elhaw Amal Taher , Kamlapurkar Shriya , Cheng Ya-Yun , Benson J Cory , Abdelnaby Ahmed Emam , Phaëton Rébécca , Wang Hong-Gang , Yang Shengyu , Sullivan Mara L.G. , St.Croix Claudette M. , Watkins Simon C. , Mullett Steven J. , Gelhaus Stacy L. , Lee Nam , Coffman Lan G. , Aird Katherine M. , Trebak Mohamed , Mythreye Karthikeyan , Walter Vonn , Hempel Nadine 
Published: September 2023

AbstractAberrant mitochondrial fission/fusion dynamics have been reported in cancer cells. While post translational modifications are known regulators of the mitochondrial fission/fusion machinery, we show that alternative splice variants of the fission protein Drp1 (DNM1L) have specific and unique roles in cancer, adding to the complexity of mitochondrial fission/fusion regulation in tumor cells. Ovarian cancer specimens express an alternative splice transcript variant of Drp1 lacking exon 16 of the variable domain, and high expression of this splice variant relative to other transcripts is associated with poor patient outcome. Unlike the full-length variant, expression of Drp1 lacking exon 16 leads to decreased association of Drp1 to mitochondrial fission sites, more fused mitochondrial networks, enhanced respiration, and TCA cycle metabolites, and is associated with a more metastatic phenotypein vitroandin vivo. These pro-tumorigenic effects can also be inhibited by specific siRNA-mediated inhibition of the endogenously expressed transcript lacking exon 16. Moreover, lack of exon 16 abrogates mitochondrial fission in response to pro-apoptotic stimuli and leads to decreased sensitivity to chemotherapeutics. These data emphasize the significance of the pathophysiological consequences of Drp1 alternative splicing and divergent functions of Drp1 splice variants, and strongly warrant consideration of Drp1 splicing in future studies.
The secreted midbody remnants, MBsomes, as a new class of mRNA nanocarrier applicable to diverse medical fields
Authors: Kim Jae Hyun , Park Sungjin , Seok Ji-Young , Cho Myung-Haing , Hwang Soon-Kyung 
Published: September 2023

ABSTRACTThe midbodysome (MBsome), a secreted remnant of midbody during cell division, is now known to play a key role in cytokinesis. It was thought that the MBsome is either released into the extracellular space or autophagically degraded by one of its daughter cells. However, recent studies have revealed that MBsomes can be maintained by cells even after cell division is complete, and that they accumulate in the cytoplasm and regulate cell proliferation and survival through integrin and epidermal growth factor receptor-dependent pathways. Here, we examined the ability of MBsomes to act as carriers of mRNAs, a novel function that has not been studied. We found that MBsomes isolated from human lung cancer and stem cells via sucrose cushion ultracentrifugation were 300–400 nm in size and stable for up to 4 days when stored at 4°C. In addition, we confirmed successful expression of the EGFP protein following incubation of the isolated MBsomes with theEGFPmRNA at room temperature. These results suggest that MBsomes have the potential to serve as mRNA carriers and therapeutic agents capable of delivering a gene-of-interest.
ASK1 promotes inflammation in senescence and aging
Authors: Odawara Takeru , Yamauchi Shota , Ichijo Hidenori 
Published: September 2023

AbstractCellular senescence is a stress-induced, permanent cell cycle arrest involved in tumor suppression and aging. Senescent cells secrete bioactive molecules such as pro-inflammatory cytokines and chemokines. This senescence-associated secretory phenotype (SASP) has been implicated in immune-mediated elimination of senescent cells and age-associated chronic inflammation. However, the mechanisms regulating the SASP are incompletely understood. Here, we show that the stress-responsive kinase ASK1 promotes inflammation in senescence and aging. ASK1 is activated during senescence and increases the expression of pro-inflammatory cytokines and chemokines by activating p38, a kinase critical for the SASP. ASK1-deficient mice show impaired elimination of oncogene-induced senescent cells and an increased rate of tumorigenesis. Furthermore, ASK1 deficiency prevents age-associated p38 activation and inflammation and attenuates glomerulosclerosis. Our results suggest that ASK1 is a driver of the SASP and age-associated chronic inflammation and represents a potential therapeutic target for age-related diseases.
An algorithmic historiography of biodiversity accounting literature
Authors: Maione Gennaro , Cuccurullo Corrado , Tommasetti Aurelio 
Published: September 2023

PurposeThe study aims to shed light on the historical and contemporary trends of biodiversity accounting literature, while simultaneously offering insights into the future of research in this sector. The paper also aims to raise awareness among accounting researchers about their role in preserving biodiversity and informing improvements in policy and practice in this area.Design/methodology/approachThe Bibliometrix R-package is used to carry out an algorithmic historiography. The reference publication year spectroscopy (RPYS) methodology is implemented. It is a unique approach to bibliometric analysis that allows researchers to identify and examine historical patterns in scientific literature.FindingsThe work provides a distinct and comprehensive discussion of the four distinct periods demarcating the progression of scientific discourse regarding biodiversity accounting. These periods are identified as Origins (1767–1864), Awareness (1865–1961), Consolidation (1962–1995) and Acceleration (1996–2021). The study offers an insightful analysis of the main thematic advancements, interpretative paradigm shifts and theoretical developments that occurred during these periods.Research limitations/implicationsThe paper offers a significant contribution to the existing academic debate on the prospects for accounting scholars to concentrate their research efforts on biodiversity and thereby promote advancements in policy and practice in this sector.Originality/valueThe article represents the first example of using an algorithmic historiography approach to examine the corpus of literature dealing with biodiversity accounting. The value of this study comes from the fusion of historical methodology and perspective. To the best of the authors’ knowledge, this is also the first scientific investigation applying RPYS in the accounting sector.
Mitigating the impact of a pandemic: a time-varying-parameter structural VAR (TVP-SVAR) and time-varying granger causality estimations
Authors: Olaoye Olumide O. , Zerihun Mulatu F. 
Published: September 2023

PurposeThe study investigates the effectiveness of government policies to mitigate the impact of a pandemic. The study adopts the small open economy of Nigeria for the following reasons. First, Nigeria is the largest economy in SSA. Second, Nigeria was also significantly impacted by the COVID-19 pandemic.Design/methodology/approachThe study employed the time-varying structural autoregressive (TVSVAR) model to control for the potential asymmetry in fiscal variables and to control for the shift in the structural shift, following a macroeconomic shock. As a form of robustness, the study also implements the time-varying Granger causality to formally assess the temporal instability of the variable of interest.FindingsThe results show that an oil price shock is an important source of macroeconomic instability in Nigeria. Importantly, the results indicate that the effects of fiscal policy are strongly time varying. Specifically, the results show that fiscal policy helps to stabilize the economy, (i.e. they help to reduce inflation and spur output growth) following macroeconomic shock. Further, the Granger test shows that fiscal policy helped to spur growth in Nigeria. The research and policy implications are discussed.Originality/valueThe study accounts for the time-varying effects of fiscal policy.
Selection ability and market timing skills of mutual fund and unit trust managers in a developing economy: evidence from Ghana
Authors: Danquah Richard , Yu Baorong 
Published: September 2023

PurposeThe study assess the selection ability and market timing skills of mutual fund and unit trust managers in Ghana.Design/methodology/approachThe study uses an improved survivorship bias-free dataset of yearly after-fee returns of all mutual funds and unit trusts operating in Ghana from January 2011 to December 2019, cumulating in nine years of quantitative fund data. The authors assess Mutual funds and Unit trusts that ever existed, “alive” or “dead,” over the sample period in the study. The authors construct factor loadings to enable the application of multifactor models in the analysis. The authors apply the unconditional versions of the Jensen alpha, Fama-French three-factor, and Carhart four-factor models to determine the selection ability and market timing skills of 32 mutual funds and 17 unit trusts. The authors deploy HAC-consistent robust standard errors to the OLS estimations to subdue the effect of heterogeneity and autocorrelation.FindingsThe results indicate that, on average, mutual funds and unit trust managers possess market timing skills but no selection ability. When the results are decomposed into fund types, fixed-income and balanced mutual fund managers possess selection ability and market timing skills.Originality/valueTo the authors' best knowledge, this study is the earliest to examine the selection ability and market timing skills of both mutual fund and unit trust managers in Sub-Saharan Africa (SSA). It is also the earliest to construct factor loadings for the Ghana stock market.
Relationships among supervisors’ socialization behaviors, newcomers’ socialization outcomes, organizational commitment, and work outcomes
Authors: Lee Andrew Sanghyun , Jacobs Ronald Lynn 
Published: September 2023

Purpose
This study aims to explore relationships among supervisors’ socialization behaviors, newcomers’ socialization outcomes, organizational commitment, and work outcomes in three large companies in Korea.

Design/methodology/approach
The research was conducted via an online survey in three large firms in Korea. The sample comprised newcomers who had worked for more than six months but less than one year in these firms. The collected data were analyzed using descriptive statistics and structural equation modeling (SEM).

Findings
Derived from SEM, the study’s results confirmed a causal relationship between supervisor’s socialization behaviors and newcomer’s work outcomes, which was fully mediated by newcomer’s socialization outcomes. However, organizational commitment was observed as a mediating variable, not a moderating variable. All types of supervisors’ socialization behaviors were related to newcomers’ socialization outcomes. Providing appropriate feedback, supporting newcomers’ development and improving social relationships were most strongly related to newcomers’ socialization outcomes.

Research limitations/implications
This study proposed a rationale for the potential effectiveness of supervisor socialization behavior training. Related variables such as supervisor training aspects and the effectiveness of supervisor training for newcomers’ organizational socialization could be examined further.

Practical implications
The study offers critical areas to consider when designing training programs focused on supervisors’ socialization behaviors. Human resource development practitioners should be aware of the importance of supervisors’ socialization behaviors for newcomers’ organizational socialization and develop a program to improve all types of supervisors’ socialization behaviors.

Originality/value
New areas of training and development for supervisors can be proposed to improve newcomer organizational socialization and, eventually, to enhance the work outcomes of newcomers.
Female consumer entrepreneurship in Asia: capabilities for micro-entrepreneurial success and the role of coaching and training
Authors: Arshad Darwina , Hodgkinson Ian R. , Hughes Paul , Khamarudin Munirah , Arshad Muhammad Zulqarnain , Bari Adibah 
Published: September 2023

Purpose
The direct selling model adopted in the beauty and cosmetics industry puts female consumer entrepreneurs at the heart of the business model. A neglected phenomenon in female entrepreneurship, this study aims to focus on female sales agents’ capabilities that are linked to sales performance and examine which capabilities might be shaped and enhanced through coaching and training in an emerging economy context.

Design/methodology/approach
Survey data were generated from a sample of 249 female sales agents who agreed to participate in a coaching and training programme run by a focal firm. Data were collected in two phases to investigate the capabilities linked to sales performance pre-intervention and the impact of coaching and training on the relationships between the capabilities and sales performance post-intervention. The time-lag data were analysed using partial least squares structural equation modelling.

Findings
For female sales agents, self-efficacy and sales experience have a significant positive effect on adaptive sales performance both before and after the coaching and training intervention. In contrast, intellectual capital and self-motivation had a non-significant relationship with sales performance before the intervention. However, after the intervention, the relationship between these variables became positive and significant.

Originality/value
The study demonstrates the effects of pre- and post-coaching and training on female consumer entrepreneurs’ capabilities and the links to sales performance. These findings add critical empirical knowledge on how female consumer entrepreneurship may be developed and the role of entrepreneurship for female empowerment in the Asian context. Collectively, the findings bring to the fore the female sphere in consumer entrepreneurship research in emerging economies.
The influence of socioeconomic factors on female entrepreneurship in Southeast Asian countries
Authors: Chikh-Amnache Sabrina , Mekhzoumi Lotfi 
Published: September 2023

Purpose
Female entrepreneurship discussions will broaden and diversify as a result of global shifts. Studies of female entrepreneurship must take into account differences between male and female entrepreneurs due to the historical, cultural and social specificity of developing countries to narrow gender gaps, identify barriers, fine-tune support systems, release dormant potential and provide information for policymaking. This paper aims to measure and estimate the most crucial socioeconomic characteristics that Southeast Asian countries leverage to advance women’s business initiatives.

Design/methodology/approach
Using a panel data model whereby the Female Entrepreneurship Indicator Score serves as the dependent variable and the ten most important socioeconomic indicators serve as the independent variables. Ten southeast Asian countries are analyzed using the panel fixed effects approach of Method of Moments Quantile Regression (MM-QR) from 1980 to 2021.

Findings
It has been found by empirical panel quantile regression using the MM-QR method that the following indicators positively affect female entrepreneurship in southeast Asian countries: the assets indicator, the pay indicator, the workplace indicator, the mobility indicator and the a woman can sign a contract in the same way as a man indicator. But the parenthood indicator, the unemployment indicator, the school enrollment indicator, the men and women have equal ownership rights to immovable property indicator and the marriage indicator all have negative effects.

Originality/value
This paper uses a new method called MM-QR to look at how the most important socioeconomic factors affect female entrepreneurship in Southeast Asian countries. The results obtained will also add to and broaden the small amount of research that has been done on female entrepreneurs in developing countries.