bluuebunny/crossref_metadata_2025_split huggingface dataset:
The predictive, preventive, and personalized medicine of insomnia: Gut microbiota and inflammation
Authors: Chen Hao-wen , Zhou Rui , Cao Bi-Fei , Liu Kuan , Zhong Qi , Huang Yi-Ning , Liu Hua-Min , Zhao Jin-Qing , Wu Xian-Bo 
Published: July 2023

Abstract
Background
The human gut microbiota (GM) has been recognized as a significant factor in the development of insomnia, primarily through inflammatory pathways, making it a promising target for therapeutic interventions. In light of the principles of primary prediction, targeted prevention, and personalized treatment medicine (PPPM), identifying of specific gut microbiota associated with insomnia and exploring the underlying mechanisms comprehensively are crucial steps towards achieving primary prediction, targeted prevention, and personalized treatment of insomnia.
Working hypothesis and methodology
We hypothesized that alterations in the composition of specific GM could induce insomnia through inflammatory response, which postulates the existence of a GM-Inflammation-Insomnia pathway. Mendelian randomization (MR) analyses were employed to examine this pathway and explore the mediative effects of inflammation. We utilized genetic proxies representing GM, insomnia, and inflammatory indicators [including 41 circulating cytokines and C-reactive protein (CRP)], specifically identified from European ancestry. The primary method used to identify insomnia-related GM and examine the medicative effect of inflammation was the inverse variance weighted method, supplemented by MR-Egger and weighted median methods. Our findings have the potential to identify individuals at risk of insomnia through screening for GM imbalances, leading to the development of targeted prevention and personalized treatment strategies for the condition.
Results
Nine genera and three circulating cytokines were identified to have causal effects on insomnia, only the associations of Clostridium (innocuum group) and β-NGF on insomnia remained significant after FDR test, OR = 1.08 (95% CI = 1.04–1.12, P = 1.45×10− 4, q = 0.02) and OR = 1.06 (95% CI = 1.02–1.10, P = 1.06×10− 3, q = 0.04), respectively. CRP were associated with increased risk of insomnia, OR = 1.05 (95% CI = 1.01–1.10, P = 6.42×10− 3). After adjusting for CRP, causal effects of Clostridium (innocuum group), Bilophila, Candidatus Soleaferrea, Coprococcus 1, Holdemania, Prevotella 7, and Rikenellaceae (RC9gut group) on insomnia were changed significantly (P > 0.05). In addition, the causal pathway from Candidatus Soleaferrea and Holdemania to insomnia were mediated by most circulating cytokines. No heterogeneity and pleiotropy were detected.
Conclusions
Our study highlights the role of specific GM alterations in the development of insomnia and provides insights into the mediating effects of inflammation. Targeting these specific GM alterations presents a promising avenue for advancing the transition from reactive medicine to PPPM in managing insomnia, potentially leading to significant clinical benefits.
Aquaporins-mediated water availability in substrates for cannabis cultivation in relation to CBD yield.
Authors: Ortiz-Delvasto Nidia , Garcia-Gomez Pablo , Carvajal Micaela , González Gloria Bárzana 
Published: July 2023

Abstract
Aims
The objective of this study was to identify the most suitable substrate for Cannabis sativa L. cultivation based on its effects on water relations and CBD production.
Methods
Biomass production, physiological parameters, mineral contents, changes in the expression levels of the PIP aquaporins and the concentration of CBD was measured in Cannabis sativa L. (var. Tiborszallasi) plants cultivated on 5 substrates with different physical-chemical characteristic under controlled conditions.
Results
The substrates available water (AW) was the main factor affecting growth and production. The efficiency of the water use was governed fundamentally by transpiration. S1 and S3 were those in which the plants grew optimally and allows plants to invest energy in secondary metabolites production acquiring high levels of CBD. The plants grown in S2 and S5, composed by coconut fiber and perlite, showed the lowest growth in agreement with low transpiration rates which reduce the water uptake. S5 substrate, with some AW still available, is forcing plants to invest energy in improve water and nutrients transport as observed by the high levels of nutrients in planta and high PIPs expression levels. S4 plants presented the highest inflorescences production and CBD content which can be attributed to plant stress due to the low levels of AW and high pH and electrical conductivity (EC).
Conclusion
The absorption of water and minerals by plants has been affected by PIP-mediated water transport, playing key roles for an optimal use of the water present in the substrates with specific isoforms involved in this responses.
Effect of GABA on seed germination and seedling growth of rapeseed under salt stress
Authors: Zhang Shuyu , Khan Aaqil , Zhao Liming , Feng Naijie , Zheng Dianfeng , Shen Xuefeng 
Published: July 2023

Abstract
In order to evaluate the impacts of gamma-aminobutyric acid (GABA) priming on rapeseed germination and seedling growth under salt stress, we conducted pot experiments using salt-sensitive rapeseed variety 'Zhongshuang 11'. To study the influence of seed priming on the morph-physiological traits, rapeseed plants were treated with 150 mm NaCl after priming of GABA (0.5, 1, 1.5, 2.0, 2.5 mM). Data on morphological traits, oxidation damage and antioxidant system were contrasted before. Our findings revealed that NaCl significantly reduced root activity, chlorophyll content and antioxidative enzymes activities, and also hindered germination, seedlings and morphological growth traits of rapeseed plants. However, priming of GABA (1.5 mM) treatment to salt-stressed plants not only increased the germination and vigor indexes but also significantly improved root length and height of rapeseed. Moreover, compared to CK2, GABA (1.5 mM) application significantly enhanced root activity and chlorophyll content of 77.02% and 38.06%, respectively. GABA (1.5 mM) reduced the H2O2 and MDA content. Furthermore, Compared to CK2, GABA (1.5 mM) significantly enhanced the antioxidant enzymes i.e SOD, POD, CAT and APX activities of 34.81%, 239.46%, 66.20%, and 57.84%, as well as increased AsA and GSH content of 89.77% and 8.90%, respectively. These findings elucidated that GABA (1.5 mM) priming not only increased the germination rate, seedlings growth and antioxidants traits but also successfully alleviate the adverse impacts of salinity stress.
What is the status of the scientific association of midwifery in Iran based on the criteria of the international midwifery confederation?
Authors: Kiani Zahra , Simbar Masoumeh 
Published: July 2023

Abstract
Background
This study aimed to evaluate the Iran Scientific Association of Midwifery (ISAM) using a translated valid version of the Member Association Capacity Assessment tool (MACAT), and also to explain the needs and the related strategies to improve ISAM capacities.
Methods
This was a mixed sequential explanatory study. The study was performed in three phases. In the first phase, MACAT was translated into Persian and then its validity was assessed. In the second descriptive phase, the capacity of ISAM was assessed by two trained evaluators using MACAT. In the third phase, with a qualitative approach and using a focused group discussion and six in-depth individual interviews with 14 key informants, the demonstrated challenges in the first phase of the study, and the related suggestions for the problem solutions were discussed. The data from the qualitative phase of the study were analyzed using directed qualitative content analysis. Lincoln and Guba’s criteria were used to confirm the trustworthiness of the data.
Results
In the first phase, the validity of MACAT was shown by S-CVI = 0.91 and I-CVI = 0.89. CVR scores of all items were above 0.62 and acceptable. In the second phase, the evaluation by MACAT showed 88 out of 96 items scored 1. The items which showed poor status were related to advocacy, shortage in resources, low contribution in decision-making in midwifery affairs. In the third phase, 83 codes in the seven dimensions were extracted. Then, 53 actions were proposed for improving ISAM capabilities.
Conclusions
ISAM is among the most potent member associations of the ICM. The most necessary actions for the ISAM capacity improvement are; considering more independence, paying attention to infrastructure improvement, and defining the mechanisms in communication with the other midwifery-related sectors and potential sponsors; involvement in making macro policies in midwifery affairs.
Effect of Electrical Muscular Stimulation on Occupational Activities: a Systematic Review and Meta-Analysis
Authors: Eldaly Sameh 
Published: July 2023

Abstract
Objective: To determine the effectiveness of electrotherapy modalities to improve muscular performance and coordination in occupational activities.
Data sources:: Search strategies were conducted on 5 Randomized control trial and one Pilot study and one Experimental proof.
Results: seven studies (151 participants) were analyzed. Movement therapy plus electrical stimulation for at least two weeks is likely to produce the best outcome in improving muscle recruitment and coordination patients with in patients with occupational activities disorders.
Conclusion: the available evidence suggests that the electrical muscular stimulation in occupational therapy can be a useful adjunct to traditional therapy approaches for improving muscle function, strength, and physical function in a variety of populations.
Time-sequential fibroblast-to-myofibroblast transition in elastin-variable 3D hydrogel environments mediated by calcium signaling
Authors: Do Nhuan T. , Lee Sun Young , Lee Yoon Seo , Lee Tae Geol , Son Jin Gyeong , Kim Se-Hwa 
Published: July 2023

Abstract
Background Fibrosis plays an important role in both normal physiological and pathological phenomena as fibroblasts differentiate to myofibroblasts. The activation of fibroblasts is determined through interactions with the surrounding extracellular matrix (ECM). However, how this fibroblast-to-myofibroblast transition (FMT) is regulated and affected by elastin concentration in a three-dimensional (3D) microenvironment has not been investigated.
Methods We developed an insoluble elastin-gradient 3D hydrogel system for long-lasting cell culture and studied the molecular mechanisms of the FMT in embedded cells by mass spectrometry along with validation through real-time PCR and immunofluorescence staining.
Results By optimizing pH and temperature, four 3D hydrogels containing fibroblasts were successfully fabricated having elastin concentrations of 0, 20, 50, and 80% in collagen. At the low elastin level (20%), fibroblast proliferation was significantly increased compared to others, and in particular, the FMT was clearly observed in this condition. Moreover, through mass spectrometry of the hydrogel environment, it was confirmed that differentiation proceeded in two stages. In the early stage, calcium-dependent proteins including calmodulin and S100A4 were highly associated. On the other hand, in the late stage after several passages of cells, distinct markers of myofibroblasts were presented such as morphological changes, increased production of ECM, and increased a-SMA expression. We also demonstrated that the low level of elastin concentration induced some cancer-associated fibroblast (CAF) markers, including PDGFR-β, and fibrosis-related disease markers, including THY1.
Conclusion Using our developed 3D elastin-gradient hydrogel system, we evaluated the effect of different elastin concentrations on the FMT. The FMT was induced even at a low concentration of elastin with increasing CAF level via calcium signaling. With this system, we were able to analyze varying protein expressions in the overall FMT process over several cellular passages. Our results suggest that the elastin-gradient system employing nonlinear optics imaging provides a good platform to study activated fibroblasts interacting with the microenvironment, where the ECM plays a pivotal role.
Mild heat treatment in vitro potentiates human adipose stem cells: delayed aging and improved quality
Authors: Ryu Chiseon , Lee Jae Young 
Published: July 2023

Abstract
Background Mesenchymal stem cells (MSCs) have gained significant attention for diverse biomedical applications, including cell-based therapy. Hence, in vitro expansion of MSCs is critical; however, in vitro MSC culture inevitably leads to significant loss of stemness, growth, and differentiation potential.
Method Effects of mild heat treatment (HT) conditions (temperature, duration, and repetition) on the characteristics of adipose tissue-derived MSCs in vitro were systematically investigated. Characteristics of the MSCs subjected to the predetermined HT conditions (41 or 44ºC, 1 h, and 2X HT) were first analyzed in a single passage using various assays. In addition, the feasibility of HT for long-term MSC culture was studied. The RNA sequencing analyses were performed to elucidate the mechanism of HT effects on MSCs.
Results A comprehensive exploration of various HT conditions revealed that specific mild HT at 41ºC or 44ºC for 1 h upregulated the expression of heat shock proteins and stemness markers and enhanced differentiation potentials. Furthermore, periodic mild HT extended the maintenance of growth rate and stemness of MSCs up to an additional 10 passages, which substantially retarded their spontaneous aging during subsequent in vitro culture. RNA sequencing analyses unveiled that HT downregulated genes associated with aging and apoptosis.
Conclusion Our study successfully demonstrated that mild HT of MSCs has positive effects on their application in various biomedical fields, enhancing their capabilities and slowing down the aging process.
Photon-phonon collaboratively pumped laser
Authors: Chen Yanfeng , Fu Yu , Liang Fei , He Cheng , Yu Haohai , Zhang Huaijin 
Published: July 2023

Abstract
In 1916, Einstein considered stimulated photon emission of electron radiation, offering the theoretical foundation for laser1, technically achieved in 19602. To date, the laser has been emerging as cutting-edge modern technology from fundamental research to daily life3-8. However, the byproduct thermal phonons, along with heat creation of ineluctable non-radiative transition, is ineffective, even playing a detrimental role in lasing process. Despite the powerful ability of phonons, e.g., Raman process9,10 or Cooper pairs in superconductors11,12, it seems impractical to use random thermal phonons to help a highly coherent laser generation far beyond the inherent fluorescence spectra. Here, we realize a photon-phonon collaboratively pumped laser enhanced by heat in a counterintuitive way. We observe a clear laser transition from phonon-free 1064 nm lasing to phonon-pumped 1176 nm lasing in Nd:YVO4 crystals, associated with the phonon-pumped electron population inversion under gradually increasing temperature. Moreover, an additional temperature threshold (Tth) appears as the trigger besides ordinary photon pump power threshold (Pth). A two-dimensional lasing phase diagram of parameter space is revealed and experimentally verified with a general threshold curve ruled by Pth = C/Tth (constant C upon loss for a given crystal), similar to Curie’s Law13. We anticipate our strategy will promote the study of laser physics via dimension extending, searching for ultrahigh-efficiency and low-threshold yet tunable and compact laser devices via this new temperature degree of freedom.
Perspectives of French General Practitioners Regarding their Role in Detecting Alcohol Consumption in Pregnant Women and Identifying Individuals with Fetal Alcohol Spectrum Disorders: A Qualitative Study
Authors: Leruste Sébastien , Delfarguiel Louise , Doray Bérénice , Loubaresse Coralie , Sennsfelder Laetitia , Maillard Thierry , Marimoutou Catherine , Spodenkiewicz Michel 
Published: July 2023

Abstract
Background: Foetal Alcohol Spectrum Disorder (FASD) is the leading cause of non-genetic mental disability and social maladjustment in children. International guidelines recommend zero alcohol during pregnancy. Reunion is the most concerned from all the French regions with an estimated Foetal Alcohol Spectrum (FAS) prevalence of 1.2‰ births. General practitioners (GPs) are at the forefront of identifying patients with FASD.
Objective: To understand how GPs identify FASD.
Methods: Qualitative study with a grounded theory approach, through semi-structured face-to-face interviews with GPs. The interviews were conducted with the aim of reaching the theoretical saturation. These were transcribed into verbatim and then analysed by four researchers to ensure the triangulation of the data.
Results: The GPs reported obstacles to identify FASD: challenges in addressing taboos and paradoxical injunctions in society, the influence of limited knowledge and experience, non-specific and highly varying symptoms, ambiguous classification and method of diagnosis involving the mobilization of a multidisciplinary team and lengthy consultations. Conversely, they felt competent to identify neurodevelopmental disorders of all causes but were concerned about the long waiting period to access to specialized care. As per GPs' perspective, it is crucial to prioritize the promotion and training aimed at enhancing the identification and coordination of care pathways for children diagnosed with neurodevelopmental disorders, such FASD.
OncoBERT: Building an Interpretable Transfer Learning Bidirectional Encoder Representations from Transformers Framework for Longitudinal Survival Prediction of Cancer Patients
Authors: Lin Hui , Ginart Jorge Barrios , Chen William , Interian Yannet , Gong Haotian , Liu Bo , Upadhaya Taman , Lupo Janine , Hong Julian , Braunstein Steve , Morin Olivier 
Published: July 2023

Abstract
Deep learning transformer models have exhibited exceptional performance in various clinical tasks, including cancer outcome prediction, when applied to electronic health records (EHR). Inspired by the success of bidirectional encoder representations from transformers (BERT) in natural language processing, we present OncoBERT, a deep transformer transfer learning framework tailored for cancer outcome prediction using unstructured clinical notes from diverse cancer sites including Glioma, Prostate, and Breast. OncoBERT adapts the BERT framework for unstructured EHR notes processing and employs a logistic regression model for binary survival prediction. Our framework analyzes aggregated EHR notes for each patient from the time of diagnosis to the initial outcome evaluation timepoint, progressively incorporating new notes for up to one year, which allows us to capture the evolving nature of cancer prognostication. In comparison to stage-based outcome models and a conventional NLP method, OncoBERT outperforms them in predicting cancer outcomes across three distinct cancer types. Furthermore, we introduce a novel interpretability pipeline that allows us to extract clinical note segments identified by the OncoBERT model as the most relevant indicators of prognosis. This pipeline enhances the interpretability of the predictive outcome, surpassing the limitations of attention weights. By providing clearer insights into the model's decision-making process, our approach improves the transparency and comprehensibility of the results, ultimately empowering clinicians and patients to make more informed decisions.
BQA: A High-performance Quantum Circuits Scheduling Strategy Based on Heuristic Search
Authors: Chen Xin-miao , Wang Shi , Ye Yong-jin , Wu Yong-zheng , Jiang Bo 
Published: July 2023

Abstract
Quantum computing is currently a research hotspot in both academia and industry. The inherent parallelism of quantum computers and the resulting powerful computing power will bring new solutions to many problems that are difficult for classical computers. However, due to the limitations of technical conditions, it is difficult to achieve full direct coupling of all qubits on a quantum chip. When compiling a quantum circuit onto a physical chip, it is necessary to ensure those two-qubit gates act on pairs of directly coupled qubits by inserting SWAP gates. It will cause great additional cost when a large number of SWAP gates are inserted, leading to the execution time of quantum circuits longer. In this paper, we designed a strategy based on the business of each individual qubit to insert SWAP gates, named Busy-Qubits-Avoid Strategy. On the one hand, we try to hide the time overhead incurred by the inserted SWAP gates by exploiting the uneven distribution of quantum gates over qubits. On the other hand, we also expect the inserted SWAP gates to make as little negative impact on subsequent two-qubit gates as possible. We designed a heuristic function which take into account both of these points. Compared with Srabe and tket, we achieved a better effect. In addition, as the number of two-qubit gates increases, better optimization results will be achieved. This implies higher execution efficiency and lower decoherence error rate.
Spatio-temporal Change of Wetland and their Effects on Wetland Biodiversity in Dinsho District, Bale Mountain Eco-Region, Southeast Ethiopia
Authors: Garedew Kefa Feye , Wedajo Mersha Alemu , Belete Mulugeta Dadi 
Published: July 2023

Abstract
Wetlands are landscape units that exist at the boundary between land and water. Wetland resources are critical to the survival of humans, plants, and animals. Wetlands, on the other hand, have declined in both time and space. As a result, the opportunities for wetland services became more limited. As a result, the current study concentrated on assessing spatiotemporal wetland changes, drivers, and their effects on wetland biodiversity in Dinsho District of Bale Zone, Southeastern Ethiopia. Landsat satellite images from 1990, 2000, 2010, and 2020 were analyzed to determine spatiotemporal wetland changes over the last three decades. To supplement remotely sensed satellite data, a household survey, key informant interviews, focus group discussions with local communities, and field observations were conducted. For satellite image analysis and display, the results were statistically analyzed using ERDAS IMAGINE 2015 and ArcGIS 10.5 software. The socioeconomic data was analyzed using SPSS 20. According to the findings, the wetland's land cover share before 30 years was 10934.25ha (16.88 percent). However, after 30 years, the land cover shares of wetlands had decreased dramatically to 6090.79 ha (10.19 percent). The spatio-temporal wetlands change analysis revealed that wetlands in the study area were reduced by 4843.46 ha (7.48 percent) between 1990 and 2020. Because wetlands are wasteland, the local community has periodically encroached on them in the study area for farmland and grazing land. Wetland changes are primarily driven by farmland expansion, overgrazing, sedimentation, and climate variability. Wetland loss has had an impact on the wetland ecosystem and biodiversity, resulting in the extinction of water birds, grasses, and tree species. In conclusion, the study shows that the magnitude of change in wetland loss in the study area increased over time and space, resulting in ecological effects in the study area. As a result, the government and stakeholders should intervene to improve sustainable wetland management by implementing appropriate management practices and raising awareness.