bluuebunny/crossref_metadata_2025_split huggingface dataset:
Readiness for return to work model-based analysis of return-to-work perception of young and middle-aged colorectal cancer patients with stoma: A descriptive qualitative study
Authors: Ren Yulan , Zhou Ying , Yang Yao , Xia Rui , Yang Yuan , Zhang Lingling , Li Hui , Tian Di , Su Xi , Wang Qiao 
Published: February 2023

Abstract
Purpose: With an increase in the number of young and middle-aged colorectal cancer (CRC) patients with stoma, understanding their perception about return to work (RTW) can guide medical professionals to provide appropriate rehabilitation strategies, which can eventually improve patients’ readiness for RTW and enable them to achieve final rehabilitation. The present study aimed to investigate the RTW-related perceptions of young and middle-aged CRC patients with stoma after surgery.
Methods: From 2021 to 2022, we conducted a basic interpretive qualitative study involving semi-structured interviews with 17 CRC patients with stoma in two grade 3A hospitals in China. This study was based on the readiness for return to work (RRTW) model. Data collection was continued until data saturation was reached, and all data were transcribed verbatim and analyzed by Colaizzi’s phenomenologicalmethod.
Results: The following three key themes were identified: (1) self-efficacy; (2) decision balance; and (3) change process. Eight subthemes were formulated that were included within the respective main themes.
Conclusion: In light of the current low self-efficacy and unsatisfactory willingness of patients with stoma about RTW, we suggest that medical staff should implement cognitive intervention and supportive intervention to improve self-efficacy and actively enhance the motivation of patients for RTW and simultaneously resolve the pertinent difficulties; this could help patients to accept the positive change process and enable their successful transition from a change process to RTW.
Plant-Inspired Adhesive and Injectable Natural Hydrogels: In Vitro and In Vivo Studies
Authors: Bohórquez-Moreno Cristian Daniel , ÖKSÜZ Kerim Emre , Dinçer Emine , Hepokur Ceylan , Şen İlker 
Published: February 2023

Abstract
The development of alternative therapeutic treatments based on the use of medicinal and aromatic plants (Juniper communis L.) has aroused interest in the medical field to find new alternatives to the conventional therapeutic treatments, which nowadays have shown problems related to bacterial resistance, high costs or sustainability in their production. The present work describes the use of hydrogels based on sodium alginate and carboxymethyl cellulose with combinations of juniperus leaves and berries extracts in order to characterize their chemical characteristics, antibacterial activity, tissue adhesion test, cytotoxicity in L929 cell line, and their effects on an in vivo model in mice in order to maximize the use of these materials in the healthcare field. It has been overall obtained an adequate antibacterial potential against S. aureus, E. coli, P. vulgaris with the use of doses above 100 mg. mL− 1 of hydrogels, likewise a low cytotoxicity in hydrogels combined with extracts has been identified according with IC50 value at 17.32 µg. mL− 1 in comparison with the higher cytotoxic activity expressed by the use of control hydrogels with a value at 11.05 µg. mL− 1. Besides, in general the observed adhesion was high to the different tissues, showing its adequate capacity to be used in different tissue typologies. Furthermore, the in-vivo results have not shown erythema and edema or other complications related with the use of the proposed hydrogels. These results suggest the feasibility of using these hydrogels in biomedical applications in reason of the observed safety.
Barriers and Enablers to Testing for Hepatitis C Infection in People Who Inject Drugs – A Scoping Review of the Qualitative Evidence
Authors: Balsom Cathy R. , Farrell Alison , Kelly Deborah V 
Published: February 2023

Abstract
Background
Injection drug use is the primary mode of transmission of hepatitis C virus (HCV) infection in the developed world and guidelines recommend screening individuals with current or history of injection drug use for HCV; however, the majority of those living with HCV in Canada are not aware of their positive status. This low level of HCV status awareness suggests that screening is not effective with current testing strategies. The aim of this review is to determine what barriers and enablers people who inject drugs (PWID) experience surrounding testing for HCV to help inform the development of an appealing testing strategy.
Methods
Comprehensive literature searches were conducted using Medline, Embase and CINAHL. Included studies investigated the barriers and enablers to testing for HCV in PWID and the experiences of PWID in testing for HCV. Studies were included if they involved people with current injection drug use or those with a history of injecting drugs, were written in the English language and set in the community. Studies were compared and common themes were coded and analyzed
Results
Nine studies were included most studies focused on barriers instead of enablers. Common barriers included self-perception of low risk for HCV, fear of diagnosis, stigma associated with IV drug use and HCV, antipathy in relation to mainstream health care services, limited knowledge about HCV, lack of rapport with provider, lack of motivation or competing priority of drug use, and limited awareness of new treatment options. Common enablers to testing included increasing awareness of HCV testing and treatment and providing positive narratives around HCV care, positive rapport with provider, accessible testing options and personalized care.
Conclusion
This scoping review determined that there has been some research in this area over the past decade though it has not been focused to specifically address this research question. Nevertheless, some common barriers and enablers to testing for HCV were observed across the available research.
Autophagy and neuroprotection in astrocytes exposed to 6-hydroxydopamine is negatively regulated by NQO2: a potential novel target in Parkinson’s disease.
Authors: Janda Elzbieta , Parafati Maddalena , Martino Concetta , William Jonahunnatha Nesson George , Reybier Karine , Mollace Vincenzo , Boutin Jean 
Published: February 2023

Abstract
Dopaminergic degeneration is a central feature of Parkinson’s disease (PD), but glial dysfunction may accelerate or trigger neuronal death. In fact, astrocytes play a key role in maintenance of the blood-brain barrier and detoxification. 6-hydroxydopamine (6OHDA) is used to induce PD in rodent models due to its specific toxicity to dopaminergic neurons, but its effect on astrocytes has been poorly investigated. Here, we show that 6OHDA dose-dependently impairs autophagy in human U373 and primary murine astrocytes in the absence of cell death. LC3II downregulation was observed 6 to 48 hours after treatment. Interestingly, 6OHDA enhanced NRH:quinone oxidoreductase 2 (NQO2) expression and activity in U373 cells, even if 6OHDA is not its substrate. The autophagic flux was restored by inhibition of NQO2 with S29434, which correlated with a partial reduction of oxidative stress in response to 6OHDA in human and murine astrocytes. NQO2 inhibition also increased neuroprotective capability of U373 cells, since S29434 protected dopaminergic SHSY5Y cells from 6OHDA-induced cell death when co-cultured with astrocytes. Silencing of NQO2 attenuated toxic effects of 6OHDA on autophagy. Finally, the analysis of Gene Expression Ominibus datasets showed elevated NQO2 gene expression in the blood cells of early-stage PD patients. These data support a toxifying function of NQO2 in dopaminergic degeneration via negative regulation of autophagy and neuroprotection in astrocytes, suggesting a novel pharmacological target in PD.
Profiling stress-triggered RNA condensation with photocatalytic proximity labeling
Authors: Zou Peng , Ren Ziqi , Tang Wei , Peng Luxin 
Published: February 2023

Abstract
Stress granules (SGs) are highly dynamic cytoplasmic membrane-less organelles that assemble when cells are challenged by stress. At different stages of assembly, various RNA molecules are sorted into SGs where they play important roles in maintaining the structural stability of SGs and regulating gene expression. Despite recent efforts of fluorescence microscopy and biochemical fractionation analysis, there still lacks a complete description of the dynamic changes in the molecular inventory of SG components during different stages of assembly and disassembly. In this study, we applied a proximity-dependent RNA labeling method, CAP-seq, to comprehensively investigate the content of local transcriptome in SGs, in the context of live mammalian cells. CAP-seq captures 457 and 822 RNAs in arsenite- and sorbitol-induced SGs in HEK293T cells, respectively, revealing that SG enrichment is positively correlated with RNA length and AU content, but negatively correlated with translation efficiency. The high spatial specificity of CAP-seq dataset is validated by single-molecule FISH imaging. We further applied CAP-seq profile RNA components in microscopically invisible SG cores, both before stress and after recovery from stress, thus mapping the dynamic changes in SG-proximal transcriptome along the time course of granule assembly/disassembly processes. Our data portray a model of AU-rich and translationally repressed SG nanostructure that are memorized long after the removal of stress.
Bioaccumulation of Persistent Organic Pollutants in Neotropical Fish Astyanax Altiparanae
Authors: Stremel Tatiana R. de O. , Silva Cleber Pinto da , Domingues Cinthia E. , Voigt Carmem Lucia , Pedroso Carlos Raphael , Vidal Carlos Magno de Sousa , Campos Sandro X. 
Published: February 2023

Abstract
The Alagados Reservoir is located within two conservation units: the Devonian Escarpment Environmental Protection Area (DEEPA) and the Campos Gerais National Park (CGNP), forming part of the Pitangui River Dam. Additionally, it is situated beneath the Guarani Aquifer, one of the largest aquifers in the world, which is an important source of groundwater for several countries in South America. Persistent Organic Pollutants (POPs) residues were detected in roe, viscera, and muscle of Astyanax altiparanae from the Alagados reservoir (Paraná State, Brazil). The presence of POPs may be related to the excessive use of regulated pesticides or even contrabanded products, which have a higher concentration than that allowed by law in Paraná State. Was observed a pattern of accumulation of p, pꞌ-DDT in Astyanax altiparanae: roe > muscle > viscera; regarding the other POPs, the accumulation pattern observed was: viscera > roe > muscle. Principal component analysis showed a proportional relationship between the highest concentrations of POPs and the width of the fish abdomen. The levels of POPs detected in Astyanax altiparanae samples did not exceed the maximum residue limits imposed by the Food and Agriculture Organization of the United Nations (FAO) and the World Health Organization (WHO), but risk quotient calculations indicated possible health risks associated with the consumption of these fish. In addition, of the POPs analyzed in regularly commercialized samples, the only pesticide detected in concentrations above the stipulated Maximum Residue Limit (MRL) by the FAO and WHO were α-Endosulfan and β-Endosulfan. As POPs may act as endocrine disruptors, low concentrations may be more effective than those of toxicological levels, concluding that the consumption of these fish can pose risks to the health of their consumers.
Leveraging community health workers as vaccinators: a case study exploring the role of Malawi’s Health Surveillance Assistants in delivering routine immunization services
Authors: Alban Rebecca , Gibson Emily , Payne Jenny , Chihana Tafwirapo 
Published: February 2023

Abstract

Background:Global chronic health worker shortages and stagnating routine immunization rates require new strategies to increase vaccination coverage and equity. As trained, trusted members of their local communities, community health workers (CHWs)are in a prime position to expand the immunization workforce and increase vaccination coverage in under-reached communities. Malawi is one of only a few countries that relies on CHWs - called Health Surveillance Assistants (HSAs) in Malawi - to administer routine immunizations, and as such offers a unique example of how this can be done.
Case Presentation: We sought to describe the operational and programmatic characteristics of a functional CHW-led routine immunization program by conducting interviews with HSAs, HSA supervisors, ministry of health officials, and community members in Malawi. This case study describes how and where HSAs provide vaccinations, their vaccination-related responsibilities, training and supervision processes, vaccine safety considerations, and the community-level vaccine supply chain. Interview participants consistently described HSAs as a high-functioning vaccination cadre, skilled and dedicated to increasing vaccine access for children. They also noted a need to strengthen some aspects of professional support for HSAs, particularly related to training, supervision, and supply chain processes. Interviewees agreed that other countries should consider following Malawi’s example and use CHWs to administer vaccines, provided they can be sufficiently trained and supported.
Conclusions: This account from Malawi provides an example of how a CHW-led vaccination program operates. Leveraging CHWs as vaccinators is a promising yet under-explored task-shifting approach that shows potential to help countries maximize their health workforce, increase vaccination coverage and reach more zero-dose children. However more research is needed to produce evidence on the impact of leveraging CHWs as vaccinators on patient safety, immunization coverage/vaccine equity, and cost-effectiveness as compared to use of other cadres for routine immunization.
Addressing Dementia Through Analysis of Population Traits and Risk Factors (ADAPTOR) using Australia’s largest cohort study: a study protocol
Authors: McNamara Martin , Dolja-Gore Xenia , Cavenagh Dominic , D'Este Catherine , Melo Luciano , Jackson Kirsten , Nepal Smriti , Cresswell Adam , Anstey Kaarin , Brodaty Henry , Welberry Heidi , Goh Anita 
Published: February 2023

Abstract

BackgroundDementia is a leading cause of disease burden in Australia, with almost half a million people living with dementia and a steady increase over time in dementia-related deaths. Strengthening the evidence base for dementia risk factors is critical for an effective and efficient public health and policy response. However, the number of Australians with dementia remains unknown, and there are significant gaps in the knowledge on risk factors in the Australian context. In this study we aim to develop reliable data on dementia incidence in Australia and investigate the associated risk factors, using a large population cohort. Specifically, we will assess the relative contribution of risk factors to dementia incidence as a basis for strengthening dementia prevention efforts.MethodsWe will use data from the 45 and Up Study that includes 267,358 residents of New South Wales, Australia, aged over 45 years, recruited between 2006-2009. To identify dementia cases we will link data from the 45 and Up Study with multiple health datasets containing information relevant to dementia case identification. We will estimate age- and sex-specific dementia incidence and model the association between dementia and risk factors related to socio-demographic characteristics, health conditions and health behaviours. We will also estimate the impact of various modifiable exposures on dementia incidence. Based on the results, we will produce a series of knowledge translation products providing advice on the contribution of identified risk factors to dementia incidence in Australia.DiscussionLinking the 45 and Up Study data to multiple health datasets provides a unique opportunity to explore the role of risk factors on dementia incidence, including modelling the effect of modifiable risk factors on dementia incidence in the Australian population. We anticipate the results from this study to guide targeted and gradated strategies for population-level dementia prevention.
Essential Features and Level of Inquiry of the Activities in the Grade 9 Science Learner’s Material
Authors: Meneses Jethromel 
Published: February 2023

Abstract

This research employed a document analysis-descriptive method to investigate the presence and level of scientific inquiry within the Grade 9 Science learner's material across four domains: Biology, Chemistry, Earth Science, and Physics. A panel of experts evaluated the material using a five-phase data analysis and a researcher-made rubric. The findings indicate that most of the analyzed science activities encompass the five essential features of inquiry, but with a higher frequency of Feature 1 (engaging learners in scientifically oriented questions) and Feature 2 (making learners prioritize evidence in responding to questions) across all activities and domains. Furthermore, the mean level of the essential inquiry features in the material falls under Category 2, indicating a slight level of student self-direction. The study suggests that the activities in the Grade 9 Science learner's material are limited in providing learners with opportunities to develop their inquiry skills. This study thus provides important implications for educational administrators and policy-makers in developing and implementing science education content.
Regional Collaborative Prediction of Air Pollutants Based on CNN-BiLSTM Model
Authors: Lu Yanan , Li Kun 
Published: February 2023

Abstract
The development of industry has brought serious air pollution problems. It is very important to establish a high-precision and high-performance air quality prediction model and take corresponding control measures. In this paper, based on four years of air quality and meteorological data in Tianjin, China, the relationship between various meteorological factors and air pollutant concentrations are analyzed, the abnormal data are detected and preprocessed. A hybrid deep learning model consisting of convolutional neural network (CNN) and bidirectional long short-term memory (BiLSTM) is proposed to predict pollutant concentrations, and the effects of three different database input modes are compared. In addition, the Bayesian optimization algorithm is applied to obtain the optimal combination of hyper-parameters for the proposed deep learning model, which makes the model have higher generalization ability. Furthermore, based on the air quality data of multi stations in the region, a regional collaborative prediction method is designed, the concept of strongly-correlated station (SCS) is defined, and the results of collaborative prediction are modified using the idea of SCS, effectively improving the accuracy of prediction.
Facile fabrication of CuFe2O4 coated with Carbon Quantum Dots nanocomposite as an efficient heterogeneous catalyst toward the reduction of nitroaniline compounds for management of aquatic resources
Authors: Naghash-Hamed Samin , Arsalani Nasser , Mousavi Seyed Borhan 
Published: February 2023

Abstract
The main objective of the current study is to scrutinize the fabrication of facile carbon quantum dots (CQDs) of glucose along with CuFe2O4-CQD nanocomposite and its catalytic reduction towards 2-Nitroaniline (2-NA) and 4-Nitroaniline (4-NA). Considering this, glucose-CQDs and nanocomposite of CuFe2O4-CQD were synthesized via hydrothermal and co-precipitation methods, respectively. Afterward, the fabricated nanocomposite as a promising nanocatalyst was employed to catalyze the 2-NA and 4-NA as a model of toxic organic pollutants into the 1,2-diaminobenzene (1,2-DAB) and 1,4-diaminobenzene (1,4-DAB) with a reducing agent of sodium borohydride (NaBH4), sequentially. The developed CQDs and magnetic nanocomposite were analyzed using FT-IR, XRD, VSM, SEM, TEM, BET surface area, BJH pore volume, and UV-Vis techniques. The results explicated that the magnetically recyclable CuFe2O4-CQD catalyst demonstrated high activity (∼ 96.7% conversion within 45 s, for 2-NA and ∼ 96.5% conversion within 18 s, for 4-NA) and efficient recyclability (up to 5 Cycles). The pseudo-second-order apparent reaction constant rate (k2) of 2-NA and 4-NA reduction were 7.12×10− 2 and 1.77×10− 1, respectively. These findings reveal that the fabricated catalyst offers a new method for the simultaneous reduction of 2-nitroaniline as well as 4-nitroaniline simply for environmental safety purposes.
Combination of olaparib and savolitinib overcomes olaparib-resistance in epithelial ovarian cancer models
Authors: Kim Min-Je , Lee Shin-Wha , Park Su-Bin , Lee Young-Jae , Kim Yong-Man 
Published: February 2023

Abstract
Background
Resistance to PARP inhibitor occurs frequently and diversely in spite of germline/somatic BRCA mutations. Receptor tyrosine kinase cMET, which is frequently overexpressed in EOC associates with PARP1 activity and cell survival pathway. We investigated whether the combination of PARP inhibitor and cMET inhibitor could overcome PARP inhibitor resistance in resistant patient-derived xenografts (PDXs).
Methods
We established acquired resistant and innate resistant PDXs, which have BRCA mutation and cMET overexpression. These resistant models were used for evaluation of combined treatment of PARP inhibitor and cMET inhibitor. Resistant PDXs were treated with Vehicle, Olapairb, Savolitinib, Combination treatment.
Results
In acquired resistant PDXs, tumor growth rate were highly increased in vehicle group than the sensitive PDXs. Contrary to sensitive group, combination treatment was more efficient to inhibit tumor growth rather than olaparib single treatment. In the innate resistant PDXs were more tolerated to olapairb than the acquired resistant PDXs. As the acquired resistant PDXs, combination treatment was most efficient to inhibited tumor growth.
Conclusion
In this study, cMET inhibition sensitized the tumor with PARP inbihitor resistance to PARP inhibitor. These results indicated that savolitinib could have synerge with the combined treatment of PARP inhibitors for EOC patients with PARP inhibitor resistant.