bluuebunny/crossref_metadata_2025_split huggingface dataset:
‘Animal Ethics Dilemma’: an interactive learning tool for university and professional training
Authors: Hanlon AJ , Algers A , Dich T , Hansen T , Loor H , Sandøe P 
Published: January 2023

Abstract‘Animal Ethics Dilemma’ is a freely available computer-supported learning tool (www.animalethicsdilemma.net or www.aedilemma.net) which has been developed primarily for veterinary undergraduates but is applicable also to students in other fields of animal science. The objectives of the computer program are to promote students' understanding of the ethics related to animal use, to illustrate ethical dilemmas that arise in animal use, to broaden students' moral imagination, and to enable students to differentiate between types of ethical argument. The program comprises five case studies: (1) the blind hens; (2) ANDi the genetically modified monkey; (3) euthanasia of a healthy dog; (4) animal slaughter; and (5) rehabilitation of seals. Special consideration has been given to enhancing the pedagogic value of the program. Students can control their learning by selecting a variety of ways to explore the program; for example, they can navigate the program using the ‘Assist Me’ option, which explains the basis of the ethical arguments. Reality text provides details of real events on which the case is based, and a glossary of terminology is available for the students to explore. Selected access to a case template is also available, enabling students and teachers to create their own case studies. Evaluation of the program has been ongoing during its development.
Welfare and mate choice in zebra finches: effect of handling regime and presence of cover
Authors: Collins SA , Archer JA , Barnard CJ 
Published: January 2023

AbstractMuch attention has been focused, quite rightly, on the welfare of laboratory rodents and farm animals but certain other groups have been less well represented in welfare research. Small birds, for example, are often kept as pets and used in a wide variety of behavioural and physiological experiments where ‘best’ housing conditions are based on advice from experienced keepers as opposed to being tested experimentally. We investigated the effects of two husbandry conditions on the welfare of captive zebra finches: a) optional cover and b) rewarded handling versus random rewards. As a correlate of welfare in the four conditions (cover + reward, cover, reward, nothing), we recorded the time to settle and perform normal behaviours after an experimenter entered the room throughout the study (ie habituation to disturbance). In addition, we measured female preference for males in the four conditions to see whether welfare situation affected attractiveness as a mate. Birds in the two conditions where a reward was provided settled most quickly; and their settling time decreased across the study. Birds provided with cover alone became more disturbed by the entry of the experimenter as the study progressed. However, the birds taking longest on average to settle were those in cages with no cover and no reward. Females preferred males in the reward conditions as mates, either due to the fact that these males settled more quickly or because less-stressed males are more attractive in some other way. Thus, rewarding birds after disturbance is an effective and simple way to improve habituation to handling and human presence. In addition, these birds are more attractive to females, implying that males more habituated to captivity may be preferred as mates. Provision of cover may help under certain circumstances, but appears paradoxically to lead to increased fearfulness over time under the conditions studied here.
The effect of pen size and an enrichment structure (elevated platform) on the performances and the behaviour of fattening rabbits
Authors: Postollec G , Boilletot E , Maurice R , Michel V 
Published: January 2023

AbstractThe effects of a (30 cm high) elevated platform as an enrichment structure on the behaviour and performance of fattening rabbits kept in groups were investigated. Three housing systems for fattening rabbits were compared using a stocking density of 15 rabbits m–2. The rabbits were housed either in large pens (3.67 m2plus a platform of 0.39 m2; with 60 rabbits per pen) or in small pens (0.503 m2plus a platform of 0.159 m2; with10 rabbits per pen), or in conventional standard cages (0.39 m2, with 6 rabbits and without any enrichment) from 31 to 72 days of age. The conventional cages without a platform were used as a control and reference model. Rabbits housed in each small pen or in each cage belonged to the same litter, and the 60 rabbits sharing the same large pen were from six or seven litters. At the end of fattening, rabbits reached the weight of 2,508 g in cages, 2,397 g in small pens, and 2,340 g in large pens; the only significant difference was daily weight gain which was better in cages than in both pens. There was no difference in growth parameters between the two types of pens. The mortality rate was less than 1% for all treatments. No sanitation problems or severe lesions were seen with rabbits even those reared in large pens and large groups. Neither housing systems, nor elevated platform affected activities such as dietary intake or resting. The use of the platform appears to depend upon the amount of space available; in a large pen, an elevated platform can be utilised as an exercise structure while in a small pen it was used more as simply extra space to occupy.
Post-release survival of hand-reared tawny owls (Strix aluco) based on radio-tracking and leg-band return data
Authors: Leighton K , Chilvers D , Charles A , Kelly A 
Published: January 2023

AbstractThe post-release survival of hand-reared tawny owls(Strix aluco)was measured using radio-tracking and leg-band return data. Of 16 birds fitted with 2.4 g radiotelemetry tags, two shed their tags after four and nine days, respectively and one bird was recovered and the tag removed. The remaining 13 birds were tracked for between 16 and 84 days (median 38). Of these, two were found dead (one emaciated and one predated) and one was recovered alive but emaciated and was subsequently euthanased. Thirty-seven percent were radio-tracked for more than six weeks, thought to be the critical period beyond which raptors will mostly survive. Of 112 birds banded between 1995 and 2005, 18 were recovered (seven live and 11 dead). Of the seven live recoveries, three were involved in road traffic collisions. Of the dead recoveries for which the cause of death was known (n = 4), all had been involved in road traffic collisions. The time elapsed between release and recovery ranged from 1–2,246 days (median 123 days). Over 65% survived for more than six weeks. The distance travelled between release and recovery ranged between 0 and 6 km (median 0). Further work is required on the effects of hand rearing on post-release survival of rehabilitated wildlife.
Perioperative stress response in dogs undergoing elective surgery: variations in behavioural, neuroendocrine, immune and acute phase responses
Authors: Siracusa C , Manteca X , Cerón J , Martínez-Subiela S , Cuenca R , Lavín S , Garcia F , Pastor J 
Published: January 2023

AbstractThe aim of this trial was to describe the behavioural, neuroendocrine, immune and acute phase stress responses in dogs undergoing elective surgery in normal, clinical practice conditions. Sixteen dogs were submitted to elective orchiectomy or ovariohysterectomy using a standardised surgical protocol. Each animal was confined to the Intensive Care Unit during pre- and post-surgery and perioperative behavioural, neuroendocrine, immune and acute phase responses were studied. Behavioural categories, cortisol, prolactin, white blood cell, C-reactive protein and haptoglobin variation were evaluated. Values at different times were compared with basal values shown by the dog in its usual environment. Communicative and explorative behaviours showed high occurrence pre-surgery and were inhibited post-surgery. Decreases in post-surgery activity, interactive behaviours and changes in waking/sleeping patterns were observed. The most sensitive marker of psychological stress, cortisol, in comparison with basal values, showed a significant increase both during pre- and post-surgery confinement in the ICU cage. Prolactin values were characterised by a significant decrease early into the post-surgery period. The immune response was characterised by long-term neutrophilia and monocytosis, but by short-term lymphopaenia and eosinopaenia, limited to the early post-operative period. With regard to the acute phase response, both C-reactive protein and haptoglobin showed a long-term increase, post-surgery. Changes in behavioural, haematological and biochemical markers showed that perioperative stress represents a major challenge to dog welfare.
Welfare assessment of captive Asian elephants (Elephas maximus) and Indian rhinoceros (Rhinoceros unicornis) using salivary cortisol measurement
Authors: Menargues A , Urios V , Mauri M 
Published: January 2023

AbstractThe measurement of salivary cortisol allows non-invasive assessment of welfare in captive animals. We utilised this technique to test the effect of zoo opening on six Asian elephants and two Indian rhinoceros at the Terra Natura Zoological Park, Alicante, Spain, during pre-opening, opening and post-opening periods. Salivary cortisol concentrations were found to be significantly higher during the opening period than during pre- and post-opening periods for both species. This method could prove a useful tool in monitoring the success of decisions taken to improve the welfare of captive animals.
Preferences of Housed Finishing Beef Cattle for Different Floor Types
Authors: Lowe D E , Steen R W J , Beattie V E 
Published: January 2023

AbstractSix pairs of steers were allowed to choose between two types of floors in a paired choice test. The four floors tested were a fully slatted floor, a fully slatted floor covered with rubber mats, a solid floor with sawdust bedding, and a solid floor with straw bedding. All combinations of floor types were tested and the choices were repeated eight times, using naïve animals. The animals were allowed 17 days to habituate, and on days 18-21 their behaviour was recorded by video for 72 hours. Straw was the most preferred floor type, followed by sawdust, then mats, and finally slats. During a second test period, rubber mats were compared with rubber strips, and no significant preferences were found.
Improving the Implementation of Advance Directives in Spain
Authors: Herreros Benjamín , Gella Pablo , Valenti Emanuele , Márquez Octavio , Moreno Beatriz , Velasco Tayra 
Published: January 2023

AbstractSince 2002, legislation in Spain has allowed for the creation and documentation of end-of-life decisionmaking. Over the intervening years, the actual implementation of such documents is very low. Through extensive analysis of the literature, this article explores the current status of the use of and attitudes toward advance directives in Spain and then proposes strategies for improvement in their implementation.
On the Replicability of Data Collection Using Online News Databases
Authors: Karstens Mikaela , Soules Michael J. , Dietrich Nick 
Published: January 2023

ABSTRACTNews databases, such as Factiva and Nexis Uni, are vital for the construction of many commonly used datasets of political events because they provide researchers with access to thousands of diverse news sources. This article raises several issues with news databases that pose a threat to the quality and replicability of data-collection efforts. We recommend best practices for using news databases to gather event data.
‘Not a cure, but helpful’ – exploring the suitability of evidence-based psychological interventions to the needs of Black, Asian and Minority Ethnic (BAME) communities
Authors: Faheem Afsana 
Published: January 2023

Abstract
Individuals from Black, Asian and Minority Ethnic (BAME) groups experience profound disparities in accessing mental healthcare, show poorer treatment outcomes, and high attrition rates when compared to their White British counterparts. Despite the national rollout of Improving Access to Psychological Therapies (IAPT) services, research exploring service users’ recovery narrative has been scarce. The aim of this study was to explore whether evidence-based psychological interventions are suitable to the needs of BAME communities. Semi-structured interviews were conducted with nine BAME service users who received evidence-based psychological treatment(s) from IAPT services. Reflexive thematic analysis (RTA) was used to analyse the data which included a six-phase process to produce a robust pattern-based analysis. Overall, three themes were generated. The first theme highlighted the importance of recognising cultural dissonance within therapy, which considered patient therapeutic expectations, therapeutic guilt, and conflicting cultural identities. The second theme identified the need for therapists to develop cultural competency. This included the importance of building therapeutic trust and exploration of patient culture within therapy. The final theme considered the road to recovery and highlighted challenges with therapeutic engagement and evaluations of therapeutic effectiveness. Overall, BAME service users felt that therapy was not a cure, but found it helpful. Clinical implications and future recommendations are discussed.

Key learning aims

(1)
To briefly introduce cross-cultural theoretical models that may assist mental health professionals to think critically about whether Western notions of therapy are suited to the needs of ethnic minority communities.

(2)
To highlight cultural challenges that may impede therapeutic success for BAME communities.

(3)
For IAPT services and practitioners to consider cultural competency training needs that may enhance service user therapeutic experiences and outcomes.

(4)
To encourage IAPT services to enhance knowledge about psychotherapy and mental health by promoting culturally sensitive psychoeducation in ethnic minority communities.
Physics‐Based Approach to Thermospheric Density Estimation Using CubeSat GPS Data
Authors: Mutschler Shaylah M. , Axelrad Penina , Sutton Eric K. , Masters Dallas 
Published: January 2023

AbstractIn Low Earth Orbit (LEO), atmospheric drag is the largest contributor to trajectory prediction error. The current thermospheric density model used in operations, the High Accuracy Satellite Drag Model (HASDM), applies corrections to an empirical density model every 3 hr using observations of 75+ calibration satellites. This work aims to improve global thermospheric density estimation by utilizing a physics‐based space environment model and precise GPS‐based orbit estimates of LEO CubeSats. The data assimilation approach presented here estimates drivers of the Thermosphere‐Ionosphere‐Electrodynamics General Circulation Model (TIE‐GCM) every 1.5 hr using CubeSat GPS information. In this work, Spire Global CubeSat data are used to demonstrate the method using only 10 satellites; the true strength of the method is its potential to exploit data already collected on large LEO constellations (hundreds of CubeSats). Precise Orbit Determination (POD) information from 10 CubeSats over 12 days is used to sense a global density field when Kp historical data show a minor and moderate geomagnetic storm in succession. This paper provides a direct comparison of estimated density, derived by our new method, to HASDM and Swarm mission derived density. A propagation analysis is also executed by comparing the CubeSat POD data to orbits propagated using our estimated density versus HASDM density. The analyses show that the estimated density is within 35% of HASDM during storm‐time conditions, and that the propagation using the estimated density yields an improvement of 26% over NRLMSISE‐00 compared to HASDM, while outperforming HASDM during the second storm peak.
Structure and Seasonal Variability of the Arctic Boundary Current North of Severnaya Zemlya
Authors: Ruiz‐Castillo Eugenio , Janout Markus , Hölemann Jens , Kanzow Torsten , Schulz Kirstin , Ivanov Vladimir 
Published: January 2023

AbstractWe assessed the spatial and temporal variability of the Arctic Boundary Current (ABC) using seven oceanographic moorings, deployed across the continental slope north of Severnaya Zemlya in 2015–2018. Transports and individual water masses were quantified based on temperature and salinity recorders and current profilers. Our results were compared with observations from the northeast Svalbard and the central Laptev Sea continental slopes to evaluate the hydrographic transformation along the ABC pathway. The highest velocities (>0.30 m s−1) of the ABC occurred at the upper continental slope and decreased offshore to below 0.03 m s−1 in the deep basin. The ABC showed seasonal variability with velocities two times higher in winter than in summer. Compared to upstream conditions in Svalbard, water mass distribution changed significantly within 20 km of the shelf edge due to mixing with‐ and intrusion of shelf waters. The ABC transported 4.15 ± 0.3 Sv in the depth range 50–1,000 m, where 0.88 ± 0.1, 1.5 ± 0.2, 0.61 ± 0.1 and 1.0 ± 0.15 Sv corresponded to Atlantic Water (AW), Dense Atlantic Water (DAW), Barents Sea Branch Water (BSBW) and Transformed Atlantic Water (TAW). 62–70% of transport was constrained to within 30–40 km of the shelf edge, and beyond 84 km, transport increases were estimated to be 0.54 Sv. Seasonality of TAW derived from local shelf‐processes and advection of seasonal‐variable Fram Strait waters, while BSBW transport variability was dominated by temperature changes with maximum transport coinciding with minimum temperatures. Further Barents Sea warming will likely reduce TAW and BSBW transport leading to warmer conditions along the ABC pathway.
Dinitrogen Emissions Dominate Nitrogen Gas Emissions From Soils With Low Oxygen Availability in a Moist Tropical Forest
Authors: Almaraz Maya , Groffman Peter M. , Silver Whendee L. , Hall Steven J. , Lin Yang , O’Connell Christine , Porder Stephen 
Published: January 2023

AbstractLowland tropical forest soils are relatively N rich and are the largest global source of N2O (a powerful greenhouse gas) to the atmosphere. Despite the importance of tropical N cycling, there have been few direct measurements of N2 (an inert gas that can serve as an alternate fate for N2O) in tropical soils, limiting our ability to characterize N budgets, manage soils to reduce N2O production, or predict the future role that N limitation to primary productivity will play in buffering against climate change. We collected soils from across macro‐ and micro‐topographic gradients that have previously been shown to differ in O2 availability and trace gas emissions. We then incubated these soils under oxic and anoxic headspaces to explore the relative effect of soil location versus transient redox conditions. No matter where the soils came from, or what headspace O2 was used in the incubation, N2 emissions dominated the flux of N gas losses. In the macrotopography plots, production of N2 and N2O were higher in low O2 valleys than on more aerated ridges and slopes. In the microtopography plots, N2 emissions from plots with lower mean soil O2 (5%–10%) were greater than in plots with higher mean soil O2 (10%–20%). We estimate an N gas flux of ∼37 kg N/ha/yr from this forest, 99% as N2. These results suggest that N2 fluxes may have been systematically underestimated in these landscapes, and that the measurements we present call for a reevaluation of the N budgets in lowland tropical forest ecosystems.
Increased cross-presentation by dendritic cells and enhanced anti-tumour therapy using the Arp2/3 inhibitor CK666
Authors: Oliveira Mariana M. S. , D’Aulerio Roberta , Yong Tracer , He Minghui , Baptista Marisa A. P. , Nylén Susanne , Westerberg Lisa S. 
Published: January 2023

Abstract
Background
Dendritic cell (DC) vaccines for cancer therapy offer the possibility to let the patient’s own immune system kill cancer cells. However, DC vaccines have shown less efficacy than expected due to failure to induce cancer cell killing and by activating T regulatory cells.

Methods
We tested if inhibition of signalling via WASp and Arp2/3 using the small molecule CK666 would enhance DC-mediated killing of tumour cells in vitro and in vivo.

Results
Using CK666 during the ex vivo phase of antigen processing of ovalbumin (OVA), murine and human DCs showed decreased phagosomal acidification, indicating activation of the cross-presentation pathway. When compared to untreated DCs, DCs treated with CK666 during uptake and processing of OVA-induced increased proliferation of OVA-specific CD8+ OT-I T cells in vitro and in vivo. Using the aggressive B16-mOVA melanoma tumour model, we show that mice injected with CK666-treated DCs and OVA-specific CD8+ OT-I T cells showed higher rejection of B16 melanoma cells when compared to mice receiving non-treated DCs. This resulted in the prolonged survival of tumour-bearing mice receiving CK666-treated DCs. Moreover, combining CK666-treated DCs with the checkpoint inhibitor anti-PD1 further prolonged survival.

Conclusion
Our data suggest that the small molecule inhibitor CK666 is a good candidate to enhance DC cross-presentation for cancer therapy.
TP73 Isoform-specific disruption reveals a critical role of TAp73beta in growth suppression and inflammatory response
Authors: Zhang Jin , Sun Wenqiang , Yan Wensheng , Kong Xiangmudong , Shen Tong , Laubach Kyra , Chen Mingyi , Chen Xinbin 
Published: January 2023

AbstractTP73 is expressed as multiple N- and C-terminal isoforms through two separate promoters or alternative splicing. While N-terminal p73 isoforms have been well studied, very little is known about p73 C-terminal isoforms. Thus, CRISPR was used to delete TP73 Exon13 (E13-KO) to induce p73α to p73β isoform switch. We showed that E13-KO led to decreased cell proliferation and migration and sensitized cells to ferroptosis, which can be reverted by knockdown of TAp73β in E13-KO cells. To understand the biological function of p73β in vivo, we generated a mouse model in that the Trp73 E13 was deleted by CRISPR. We showed that p73α to p73β isoform switch led to increased cellular senescence in mouse embryonic fibroblasts. We also showed that E13-deficient mice exhibited shorter life span and were prone to spontaneous tumors, chronic inflammation and liver steatosis as compared to WT mice. Additionally, we found that the incidence of chronic inflammation and liver steatosis was higher in E13-deficient mice than that in Trp73-deficient mice, suggesting that p73β is a strong inducer of inflammatory response. Mechanistically, we showed that TAp73β was able to induce cysteine dioxygenase 1 (CDO-1), leading to cysteine depletion and subsequently, enhanced ferroptosis and growth suppression. Conversely, knockdown of CDO-1 was able to alleviate the growth suppression and ferroptosis in E13-KO cells. Together, our data suggest that at a physiologically relevant level, TAp73β is a strong inducer of growth suppression but insufficient to compensate for loss of TAp73α in tumor suppression due to aberrant induction of inflammatory response and liver steatosis.