bluuebunny/crossref_metadata_2025_split huggingface dataset:
Diversity of Anastrepha spp. (Diptera: Tephritidae) in the Chaco Biome
Authors: de Lima Jéssica Quéren Alves , Nascimento José Nicácio , Uchoa Manoel Araécio , Garcia Flávio Roberto Mello 
Published: August 2023

Abstract
Species of the genus Anastrepha cause injuries to several fruits and vegetables in the Nearctic and Neotropical regions. Among these species, many are present in Brazil. In this study, we surveyed the existing Anastrepha species in the Brazilian Chaco Biome. And we evaluated possible influences of climate and vegetation on the occurrence of Anastrepha spp. For this, we used McAlpine traps and hydrolyzed corn protein as bait. Collections were carried out in three distinct floristic compositions in the Chaco biome, from permanently dry areas to flooded sites. We identified Anastrepha fraterculus (Wiedemann, 1830), A. sororcula Zucchi 1979, A. undosa Stone 1942d daciformis Bezzi 1909. The dry and flooded environment did not influence the occurrence of Anastrepha species. The highest probability of occurrence was in vegetation with an intermediate density of the distribution of plants, between 25 and 75%. Being 25% low and 25% high of the total. The presence of multiple hosts increases the number of species. The richness and abundance of Anastrepha species are low in the Brazilian Chaco when compared to other native environments.
SERNA: A Stacking-Based Classification Algorithm for Early Prediction of ICU Needs for COVID-19 Patients
Authors: Fili Mohammad , Mohammadiarvejeh Parvin , Hu Guiping 
Published: August 2023

Abstract
The COVID-19 pandemic exerted an unprecedented strain on healthcare systems, with millions of hospitalizations and intensive care unit (ICU) admissions. The overwhelming demand for ICU beds necessitates the efficient allocation of resources and early prediction of patients’ ICU needs. In this paper, we introduce SERNA (Stacked Ensemble using Regional and Neighborhood Assessment), a novel stacking-based classification algorithm that predicts ICU needs within the first 2 hours of patient admission. The SERNA algorithm employs novel procedures to create new feature sets, establishing connections between data point locations and learners’ local performances. These generated features are then fed into a meta-learner, which strategically assigns higher weights to strong learners and lower weights to weaker ones. We evaluated the proposed algorithm using COVID-19 ICU admission data, comparing its performance against various baseline models. Remarkably, the SERNA algorithm achieved a recall of 90% and an AUC of 80%, surpassing all baseline models. It exhibited an improvement in accuracy by 3%, precision by 1%, recall by 14%, F1 score by 6%, and AUC by 4%. Notably, these results were achieved by utilizing data only from the first 2 hours after admission, enabling a crucial reduction in reaction time of 10–22 hours compared to previous studies.
Exposure of Caralluma tuberculata to Biogenic Selenium Nanoparticles as in vitro rooting agent: Stimulates Morpho-Physiological and Antioxidant Defense System
Authors: Ali Amir , Mashwani Zia-ur-Rehman , Raja Naveed Iqbal , Mohammad Sher , Luna-Arias Juan Pedro 
Published: August 2023

Abstract
The commercial-scale production of Caralluma tuberculata faces significant challenges due to lower seed viability and sluggish rate of root growth in natural conditions. To overcome these obstacles, using phyto mediated selenium nanomaterials as an invitro rooting agent in plant invitro cultures is a promising approach to facilitate rapid propagation and enhance the production of valuable therapeutic compounds. This study aimed to investigate the impact of phytosynthesized selenium nanoparticles (SeNPs) on the morphological growth attributes, physiological status, and secondary metabolite fabrication in in vitro micro propagated Caralluma tuberculata. The results demonstrated that a lower dose of SeNPs (100 µg/L) along with Plant growth regulators (IBA: 1 mg/L) had an affirmative effect on growth parameters and promoted earliest root initiation (4.6±0.98 days), highest root frequency (68.21±5.12%), number of roots (6.3±1.8), maximum fresh weight (710±6.01 mg) and dry weight (549.89±6.77 mg). However, higher levels of SeNPs (200 and 400 µg/L) in the growth media proved detrimental to growth and development. Further, stressed caused by SeNPs at 100 ug/L along with PGRs (IBA: 1 mg/L) produced higher level of total chlorophyll contents (32.66± 4.36 ug/ml), while cultures exposed to 200 ug/L SeNPs alone exhibited maximum amount of Proline contents (10.5± 1.32 ug/ml). Interestingly, exposure to 400 µg/L SeNPs induced a stress response in the cultures, leading to increased levels of total phenolic content, total flavonoid content, and antioxidant activity (3.4 ± 0.052, 1.8 ± 0.034 and mg/g dry weight: DW and 82 ± 4.8%). Furthermore, the combination of 100 µg/L SeNPs and plant growth regulators (1 mg/L IBA) led to accelerated enzymatic antioxidant activities, including superoxide dismutase (SOD = 4.4 ± 0.067 U/mg), peroxidase dismutase (POD = 3.3 ± 0.043 U/mg), catalase (CAT = 2.8 ± 0.048 U/mg), and ascorbate peroxidase (APx = 1.6 ± 0.082 U/mg). This is the first report that highlights the efficacy of SeNPs in culture media and presents a promising approach for the commercial propagation of C. tuberculata with strong antioxidant defense system in vitro.
Improved Prediction of Clinical Pregnancy Using Artificial Intelligence with Enhanced Inner Cell Mass and Trophectoderm Images
Authors: Kim Hyung Min , Ko Taehoon , Kang Hyoeun , Choi Sungwook , Park Jong Hyuk , Chung Mi Kyung , Kim Miran , Kim Na Young , Lee Hye Jun 
Published: August 2023

Abstract
This study aimed to assess the performance of an artificial intelligence (AI) model for predicting clinical pregnancy using enhanced inner cell mass (ICM) and trophectoderm (TE) images. In this retrospective study, we included static images of 2,555 day-5-blastocysts from seven in vitro fertilization centers in South Korea. The main outcome of the study was the predictive capability of the model to detect clinical pregnancies (gestational sac). Compared to the original embryo images, the use of enhanced ICM and TE images improved the average area under the receiver operating characteristic curve for the AI model from 0.716 to 0.741. Additionally, a gradient-weighted class activation mapping analysis demonstrated that the AI model utilizing the enhanced ICM and TE images was able to extract features from crucial areas of the embryo, including the ICM and TE, in 99% (506/512) of the cases. In contrast, the AI model trained on the original images focused on the main areas in only 86% (438/512) of the cases. Our results highlight the potential efficacy of utilizing ICM- and TE-enhanced embryo images in AI models for the prediction of clinical pregnancy.
Ability of nitrogen-fixing bacteria to alleviate drought stress in cowpea varies depending on the origin of the inoculated strain
Authors: Correa Sulamita Santos , Pacheco Rafael Sanches , Viana Guilherme Caldieraro , Vidal Márcia Soares , Xavier Gustavo Ribeiro , Simoes-Araujo Jean Luiz 
Published: August 2023

Abstract

Background and Aims
Drought is one of the main causes of global crop decline. Plant growth-promoting rhizobacteria enhance plant tolerance to adverse environmental conditions. This study aimed to determine whether the rhizobacteria Microvirga vignae (BR 3296 and BR 3299) and Bradyrhizobium sp. (BR 3301) can maintain cowpea growth under drought stress.
Methods
We analyzed biomass, nodulation, nitrogen accumulation, and physiological traits of the inoculated plants. Rhizobacterial strains were assessed for exopolysaccharide (EPS) and indole acetic acid (IAA) production, growth, and biofilm formation in a water-stress medium induced by polyethylene glycol (PEG)-6000. The expression of genes associated with abscisic acid (ABA) biosynthesis in root nodules was also investigated.
Results
All evaluated strains were grown in a culture medium supplemented with PEG. M. vignae strains exhibited increased biofilm formation and EPS production, while Bradyrhizobium showed high IAA production. Cowpea plants inoculated with Bradyrhizobium exhibit higher levels of nodulation, biomass, and nitrogen accumulation. Conversely, M. vignae strains were more efficient at alleviating drought stress and maintaining nodulation, biomass, nitrogen accumulation, and stomatal conductance similar to well-watered plants. Drought-inducible genes were more strongly upregulated in the nodules of plants inoculated with Bradyrhizobium than in those inoculated with M. vignae.
Conclusion
Our results suggest that M. vignae strains, isolated from a semi-arid region, help plants withstand water-stress, whereas the strain of Bradyrhizobium sp. isolated from a wet region did not effectively alleviate drought stress. However, Bradyrhizobium sp. conferred growth and nitrogen accumulation to cowpea superior to M. vignae and like plants supplied with nitrogen fertilizer.
The effect of clinical nurses’ job-esteem on work engagement: Identifying mediating and moderating effects of positive nursing organizational culture
Authors: Cha Ji-Eun , Choi Eun-Hi 
Published: August 2023

Abstract
Background
Nursing services play an important role in providing quality healthcare. However, the association of positive nursing organizational culture with job esteem and work engagement of clinical nurses is not well established. In this study, we assessed the mediating and moderating effects of positive nursing organizational culture and job esteem on work engagement among clinical nurses.
Methods
The study adopted a descriptive correlational design. A total of 200 nurses with more than six months’ experience at two university hospitals in Korea participated in the study. Data were collected using a questionnaire which comprised questions on general characteristics of the participants, positive nursing organizational culture, the Job-Esteem Scale for Korean Nurses, and the Utrecht Work Engagement Scale.
Results
The results revealed that the direct effect of job esteem on work engagement, indirect effect via positive nursing organizational culture, and total effect were statistically significant. The moderating effect of positive nursing organizational culture on job esteem was not statistically significant.
Conclusion
The results highlight the need for a development plan to increase job esteem and create a positive nursing organizational culture to improve the work engagement of clinical nurses. Nursing colleges also need to implement measures to improve job esteem and develop step-by-step education programs, interventions, and policy support considering clinical experience. Moreover, establishment of fair procedures and appropriate compensation may boost nurses’ morale leading to increased work engagement.
Study on different mass ratio of the cathode and anode electrode of LiMn2O4//AC lithium ion capacitor
Authors: Fang Wenying , Yan Liangliang , An Zhongxun , Xia Hengheng 
Published: August 2023

Abstract
Lithium ion capacitors have high energy density and power density, among of which lithium manganate is low cost of intrinsic value, simultaneously the recovery technology of manganese is relatively mature. Therefore, it has special advantages in terms of economy. This paper applies for LiMn2O4 as the cathode electrode and AC as the anode electrode to explore the influence of the mass ratio of cathode and anode electrodes on the electrochemical performance of LiMn2O4//AC lithium ion capacitors. The results show that when the mass ratio of cathode and anode electrodes is 1:1, the specific capacity of the lithium ion capacitor can reach maximized, up to 24.33 mAh g-1. Meanwhile, the cathode electrode potential is below 4.3V, the anode electrode potential is above 1.6V as well as the rate performance and self-discharge performance are the best. At a voltage range of 0.3-2.5V, the energy density can reach 35.5 Wh/kg at a power density 34.9 W/kg. When the mass ratio of the cathode and anode electrodes is less than 1:1, the cathode electrode is insufficient. The capacity is basically not attenuated after 500 cycles, but the electrochemical impedance is large and the polarization is rather obvious. The XRD diffraction peak is weakened and the surface morphology of the electrode is blurred with cracking material particles. The study suggests that the capacity ratio of cathode and anode has a crucial effect on the electrical performance of lithium ion capacitors.
Comparative mitochondrial genome brings insights to slight variation in gene proportion and large intergenic spacer and phylogenetic relationship of mudskipper species.
Authors: Muhala Valdemiro , Guimarães-Costa Aurycéia , Bessa-Silva Adam , Rabelo Luan , Carneiro Jeferson , Macate Isadola , Watanabe Luciana , Balcázar Oscar , Gomes Grazielle , Vallinoto Marcelo , Sampaio Iracilda 
Published: August 2023

Abstract
Fish mitochondrial genome have been largely studied worldwide for evolutionary and other genetic purposes and the structure and gene organization are commonly conservative. However, several studies have demonstrated that this scenario may present variations in some taxa, showing differentiation on the gene rearrangement. In this study, the complete mitogenome of terrestrial fish Boleophthalmus dussumieri was generated and compared with other species of the Exudercidae fishes. The newly complete mitogenome generated is circular and 16,685 pb of length, and it contained 13 protein-coding genes (PCGs), two ribosomal RNA (rRNAs), 22 transfer RNA genes (tRNAs), and one control region (CR), with high conservative structure, like other Mudskippers. Most of the PCG showed similar codon usage bias. The gene length was found to be different specially for the CR, 12S rRNA gene and ND5 gene in some taxon. All the Boleophthalmus species showed a gene duplication in the CR, except for B. dussumieri. The phylogenetic analysis grouped all mudskippers species as a monophyletic group and the analysis positioned the Periophthalmus clade as the most basal in the terrestrial fishes. This finding provides basis and brings insights for gene variation, gene rearrangements and replications showing evidence for variety of mitochondrial structure diversity within mudskippers.
WITHDRAWN: Stability analysis of rainfall-induced landslides: A case study of a hilly area in Bangladesh
Authors: Hossain Md Monir , Hasan Md Tanvir , Hasanath Md Abul , Hore Ripon 
Published: August 2023

Abstract
This investigation examines the landslide due to rain in the Chattogram Hill Tracts of Bangladesh, including its origins, impacts, and the role played by the local geology and environment. Thus, designing early warning systems for such threats requires an in-depth understanding of the geo-environmental properties of soil slopes under heavy rainfall. In the past, when it rained more than 150–250 mm, localized slope collapses and large-scale debris floods occurred. Both extensive landslides and small-scale slope collapses have been seen. As a result, studies of soil-water characteristics have been conducted, followed by laboratory testing at the impacted sites. While hill cutting, deforestation, and unplanned human settlements contribute to landslide disasters, the study found that rainfall and soil type (fine-grained silt) were the most critical factors behind landslides. The slope stability equation was combined with a numerical model to pinpoint the issue's root. When it rains heavily, the soil becomes more saturated than usual, and its suction and shear strengths fall, as shown by analytical investigations and computer models. The slopes of the hills become precarious as a consequence. However, the mechanism has been demonstrated, considering some simplified assumptions. In contrast, it has been noticed that most of the landslides occurred when the degree of saturation was maximum and the safety factor was nearly 0.4 to 0.9; on the other hand, the safety factor was 1.8 to 2.0 in the dry condition. These experimental findings might be implemented in early warning systems for flash floods and landslides.
Mechanism of fluoxetine downregulation of circMap2k1 to alleviate neurological function after ischemic stroke
Authors: Zhang Hui , Deng Jian , He Yibo , Cai Zhili , He Yitao 
Published: August 2023

Abstract
Background
Ischemic stroke (IS) is known for its high incidence, disability and mortality, and there is an urgent need to investigate the pathophysiological mechanisms and develop novel treatment strategies. We aimed to investigate the mechanisms of the novel circMap2k1/miR-135b-5p/Pidd1 axis in the treatment of IS progression with fluoxetine.
Methods
The middle cerebral artery occlusion (MCAO) model was established, followed by fluoxetine treatment and injecting adeno-associated viruses (AAV)-sh-ctr and AAV-sh-circMap2k1 into bilateral hippocampal tissues of rats. Then cerebral infarction area, weight, Longa score, and neurological injury were evaluated. Dual-luciferase reporter gene assay was employed to confirm the binding between miR-135b-5p and Pidd1. ELISA was performed to measure the concentrations of inflammatory factors TNF-α, IL-6, and IL-1β in the plasma. Finally, we verified the role of circMap2k1 in cellular experiments by overexpression of circMap2k1. Cell viability was assessed using CCK-8 assay, while apoptosis was measured by flow cytometry.
Results
Knockdown of circMap2k1 enhanced the therapeutic effect of fluoxetine on IS injury (cerebral infarction area, weight, and Longa score) in rats. Then knockdown of circMap2k1 enhanced the protective effect of fluoxetine on neurological injury after IS in rats. Dual-luciferase reporter gene assay confirmed the targeting of miR-135b-5p to Pidd1. Additionally, fluoxetine deactivated the adsorption of miR-135b-5p by downregulating circMap2k1, and miR-135b-5p further exerts its inhibitory effect on Pidd1, and finally attenuares the inflammatory response caused by microglial polarization after IS. Cell experiments revealed that overexpression of circMap2k1 repressed cell viability and promoted cell apoptosis.
Conclusions
Fluoxetine downregulated circMap2k1 to ameliorate neurological injury and inflammatory responses induced by microglia polarization after IS.
RAS-activated PI3K/AKT signaling sustains cellular senescence in experimental models of psoriasis via P53/P21 axis
Authors: Albanesi Cristina , Mercurio Laura , Bailey Jacob , Glick Adam , Dellambra Elena , Scarponi Claudia , Pallotta Sabatino , Madonna Stefania 
Published: August 2023

Abstract
Psoriasis is a chronic immune-mediated skin disease in which upper epidermal keratinocytes exhibit a senescent-like phenotype. In psoriatic skin, a variety of inflammatory cytokines can activate intracellular pathways including phosphatidylinositol 3-kinase (PI3K)/AKT signaling and RAS effectors. AKT and RAS participate to cellular senescence, but currently their role in senescence responses occurring in psoriasis have not yet been investigated. Here, we found AKT hyperactivation associated to the upregulation of senescence markers in psoriatic keratinocyte cultures subjected to multiple passages to promote senescence in vitro, as well as in skin lesions of patients affected by psoriasis. AKT-induced senescence was sustained by constitutive RAS activation, and PI3K/AKT pharmacological inhibition contrasted senescence processes induced by cytokines in psoriatic keratinocyte cultures. Additionally, RAS overexpression in the suprabasal epidermis in a murine model of psoriasis was accompanied by AKT upregulation, increase of senescence marker expression and by skin inflammation. In this model, both senescence and inflammation were significantly reduced by selective AKT inhibition. Therefore, targeting RAS-AKT pathway could be a promising novel strategy to counteract multiple psoriasis symptoms.
Which protocol for prostate biopsies in patients with a positive MRI? Interest of systematic biopsies by sectors.
Authors: Zambon Audrey , Nguyen Truong An , Fourcade Alexandre , Segalen Tristan , Saout Kevin , Deruelle Charles , Joulin Vincent , Tissot Valentin , Doucet Laurent , Fournier Georges , Valeri Antoine 
Published: August 2023

Abstract
BACKGROUND Current prostate biopsy (PBx) protocol for prostate cancer (PCa) diagnosis is to perform systematic biopsies (SBx) combined with targeted biopsies (TBx) in case of positive MRI (i.e PI-RADS ≥3). To assess the utility of performing SBx in combination with TBx, we determined the added value of SBx brought to the diagnosis of PCa according to their sextant location and MRI target characteristics.
METHODS In our local prospectively collected database, we conducted a single-center retrospective study including all patients with a suspicion of PCa, who underwent transrectal ultrasound guided (TRUS) prostate biopsies (PBx) with a prior MRI and a single lesion classified as PI-RADS ≥3. We have characterized the SBx according to their location on MRI: same sextant (S-SBx), adjacent sextant (A-SBx), ipsilateral side (I-SBx) and contralateral side (C-SBx). The added value of SBx and TBx was defined as any upgrading to significant PCa (csPCa) (ISUP ≥2).
RESULTS 428 patients were included in the study. The added value of SBx was 10% overall. Regarding the lesion location and the SBx sextant, the added value of SBx was: 5.1% for S-SBx, 5.6% for A-SBx, 4.9 % for I-SBx and 2.1% for C-SBx. The overall added value of SBx was 6.0% for PI-RADS 3 lesions, 15% for PI-RADS 4 lesions and 6.7% for PI-RADS 5 lesions (p = 0,017). The added value of SBx for contralateral side was 1.7% (2/117), 3.1% (6/191) and 0.8% (1/120) for PI-RADS 3, PI-RADS 4 and PI-RADS 5 lesions, respectively (p = 0,4). The added value of SBx was lower when the number of TBx was higher (OR 0.56; CI95% 0.38-0.82; p = 0.003).
CONCLUSIONS Our results suggest that the utility of performing SBx in the contralateral lobe toward the MRI lesion was very low, supporting that they might be avoided.
Multicenter prospective phase II trial of concurrent chemoradiotherapy with weekly low-dose carboplatin for cisplatin-ineligible patients with advanced head and neck squamous cell carcinoma
Authors: Ueki Yushi , Ohshima Shusuke , Yokoyama Yusuke , Takahashi Takeshi , Shodo Ryusuke , Yamazaki Keisuke , Ohtaki Kohei , Saijo Kohei , Tanaka Ryoko , Togashi Takafumi , Sato Yuichiro , Takano Satoshi , Omata Jo , Takahashi Nao , Okabe Ryuichi , Horii Arata 
Published: August 2023

Abstract
Background
The optimal chemotherapy regimen in concurrent chemoradiotherapy (CCRT) for cisplatin-ineligible head and neck squamous cell carcinoma (HNSCC) has not been established. We aimed to evaluate the feasibility, efficacy, and safety of CCRT with weekly low-dose carboplatin for the treatment of advanced HNSCC in patients who are cisplatin-ineligible.
Methods
This prospective phase II study enrolled adult patients (age ≥ 20 years) with HNSCC receiving whole-neck irradiation including bilateral levels II–IV and who were aged (≥ 75-year-old patients with 40 mL/min estimated glomerular filtration rate [eGFR] or better) or had renal dysfunction (< 75-year-old patients with 30–60 mL/min eGFR). Carboplatin was administered weekly in the area under the plasma concentration-time curve = 2.0 for up to seven cycles during concurrent radiotherapy (70 Gy/35 Fr). The primary endpoint was the completion rate of CCRT. Secondary endpoints included overall response rate and incidence of adverse events.
Results
Among the 30 patients enrolled, 28 were males. The median age was 73.5 years. Seventeen patients were <75 years whereas 13 were ≥75 years old. The completion rate of CCRT was 90%. Overall response rate was 90%. Grade 3 adverse events that occurred in 10% or more patients were oral/pharyngeal mucositis (47%), leukocytopenia (20%), and neutropenia (10%). Grade 4 adverse events occurred in one patient (elevation of alanine aminotransferase level). No treatment-related deaths occurred.
Conclusion
CCRT with weekly low-dose carboplatin is a promising treatment option, with favorable feasibility, efficacy, and acceptable toxicity, for patients who are cisplatin-ineligible with advanced HNSCC.