bluuebunny/crossref_metadata_2025_split huggingface dataset:
Developing a novel positronium biomarker for cardiac myxoma imaging
Authors: Moskal Paweł , Kubicz Ewelina , Grudzień Grzegorz , Czerwiński Eryk , Dulski Kamil , Leszczyński Bartosz , Niedźwiecki Szymon , Stępień Ewa Ł. 
Published: March 2023

Abstract
Purpose
Cardiac myxoma (CM), the most common cardiac tumor in adults, accounts for 50–75% of benign cardiac tumors. The diagnosis of CM is often elusive, especially in young stroke survivors and transthoracic echocardiography (TTE) is the initial technique for the differential diagnostics of CM. Less invasive cardiac computed tomography (CT) and magnetic resonance imaging (MRI) are not available for the majority of cardiac patients. Here, a robust imaging approach, ortho-Positronium (o-Ps) imaging, is presented to determine cardiac myxoma extracted from patients undergoing urgent cardiac surgery due to unexpected atrial masses. We aimed to assess if the o-Ps atom, produced copiously in intramolecular voids during the PET imaging, serves as a biomarker for CM diagnosing.

Methods
Six perioperative CM and normal (adipose) tissue samples from patients, with primary diagnosis confirmed by the histopathology examination, were examined using positron annihilation lifetime spectroscopy (PALS) and micro-CT. Additionally, cell cultures and confocal microscopy techniques were used to picture cell morphology and origin.

Results
We observed significant shortening in the mean o-Ps lifetime in tumor with compare to normal tissues: an average value of 1.92(02) ns and 2.72(05) ns for CM and the adipose tissue, respectively. Microscopic differences between tumor samples, confirmed in histopathology examination and micro-CT, did not influenced the major positronium imaging results.

Conclusions
Our findings, combined with o-Ps lifetime analysis, revealed the novel emerging positronium imaging marker (o-PS) for cardiovascular imaging. This method opens the new perspective to facilitate the quantitative in vivo assessment of intracardiac masses on a molecular (nanoscale) level.
Personalization of 99mTc-sestamibi activity in SPECT/CT myocardial perfusion imaging with the cardiofocal SmartZoom® collimator
Authors: Verrecchia-Ramos Emilie , Morel Olivier , Beauchat Valérie , Denet Sylvie , Djibo Sidikou Abdourahamane , Ginet Merwan , Pfletschinger Estelle , Teodor Luminita , Trombowsky Maud , Verdier Jeany , Vère Christelle , Retif Paul , Mahmoud Sinan Ben 
Published: March 2023

AbstractBackgroundPatient radioprotection in myocardial perfusion imaging (MPI)-SPECT is important but difficult to optimize. The aim of this study was to adjust injected activity according to patient size—weight or BMI—by using a cardiofocal collimator camera.MethodsThe correlation equation between size and observed counts in image was determined in patients who underwent stress Tc-99m-sestamibi MPI-SPECT/CT with a cardiofocal collimator-equipped conventional Anger SPECT/CT system. Image quality analyses by seven nuclear physicians were conducted to determine the minimum patient size-independent observed count threshold that yielded sufficient image quality for perfusion-defect diagnosis. These data generated an equation that can be used to calculate personalized activity for patients according to their size.ResultsAnalysis of consecutive patients (n = 294) showed that weight correlated with observed counts better than body mass index. The correlation equation was used to generate the equation that expressed the relationship between observed counts, patient weight, and injected activity. Image quality analysis with 50 images yielded an observed count threshold of 22,000 counts. Using this threshold means that the injected activity in patients with < 100 kg would be reduced (e.g., by 67% in 45-kg patients). Patients who are heavier than 100 kg would also benefit from the use of the threshold because although the injected activity would be higher (up to 78% for 150-kg patients), good image quality would be obtained.ConclusionsThis study provided a method for determining the optimal injected activity according to patient weight without compromising the image quality of conventional Anger SPECT/CT systems equipped with a cardiofocal collimator. Personalized injected activities for each patient weight ranging from 45 to 150 kg were generated, to standardize the resulting image quality independently of patient attenuation. This approach improves patient/staff radioprotection because it reduces the injected activity for < 100-kg patients (the majority of patients).
“Black People Like Me”: A virtual conference series to engage underserved patients with asthma in patient centered outcomes research
Authors: Graham LeRoy , Hart Mary , Stinson Michael , Moise Rhoda , Mitchell Lynda , Winders Tonya A. , Gardner Donna D. 
Published: March 2023

Abstract
Background
In response to racial inequity in asthma, asthma-related research among diverse patients is vital. However, people from historically marginalized groups are underrepresented in clinical and patient-centered outcomes research (PCOR). The “Black People Like Me” (BPLM) virtual conference series was developed to: (1) engage Black patients with asthma and their caregivers in education and discussions about asthma, and (2) encourage involvement in PCOR. Education about COVID-19 and COVID-19 vaccination was also incorporated.

Methods
The Project Advisory Group consisting of Black patients, clergy, physicians, and a program evaluator met monthly to develop BPLM. The program consisted of free one-hour virtual sessions held monthly for 6 months. BPLM was promoted through the Allergy & Asthma Network website, emails, social media, and personal contacts with a recruitment goal of ≥ 100 Black patients with asthma or caregivers. Program evaluations, interactive polling questions during each session, and participant pre- and post-session tests were conducted.

Results
Sessions averaged 658 participants including Black patients, family members, caregivers, Black clergy, health care providers, and other concerned community. Overall, 77% of participants strongly agreed with satisfaction with the sessions. Pre- and post-tests demonstrated that participants exhibited growth in knowledge regarding asthma risk, PCOR, and PCOR research opportunities for patients, exhibited preexisting and sustained knowledge regarding COVID-19 vaccination and side effects, and demonstrated an increased sense of empowerment during healthcare visits.

Conclusions
BPLM demonstrated that a virtual platform can successfully engage Black communities. Incorporating clergy and religious organizations was critical in developing the trust of the Black community towards BPLM.
Association between diet history and symptoms of individuals having recovered from COVID-19
Authors: Sahloul Ola T. , Sahloul Talaat M. 
Published: March 2023

Abstract
Background
Many studies show that people who eat a balanced diet have stronger immunity. The present work aimed to identify the effects of the diet history of COVID-19 patients having recovered from the disease on the occurrence and severity of symptoms.

Methods
The study sample consisted of 346 individuals aged 20–65 years. The participants’ data and answers to an electronic questionnaire regarding their diet history and symptoms were collected. The study focused on four hard symptoms (fever, body pain, cough, and dyspnoea) to investigate the relationship between these symptoms and the consumption of specific immunity foods.

Results
Symptoms were reported by 88.1% of the participants eating none of the foods investigated, whereas 85.54% and 85.55% of the individuals with little or intensive food intake, respectively, experienced symptoms.

Conclusions
Intake of specific functional foods might slightly reduce the occurrence of some symptoms.
CCNE1 is a predictive and immunotherapeutic indicator in various cancers including UCEC: a pan-cancer analysis
Authors: Zheng Xingyu , Chen Lingli , Liu Wenlu , Zhao Shuangshuang , Yan Ye , Zhao Jianzhen , Tian Wenyan , Wang Yingmei 
Published: March 2023

Abstract
Background
CCNE1 plays an important oncogenic role in several tumors, especially high-stage serous ovarian cancer and endometrial cancer. Nevertheless, the fundamental function of CCNE1 has not been explored in multiple cancers. Therefore, bioinformatics analyses of pan-cancer datasets were carried out to explore how CCNE1 regulates tumorigenesis.

Methods
A variety of online tools and cancer databases, including GEPIA2, SangerBox, LinkedOmics and cBioPortal, were applied to investigate the expression of CCNE1 across cancers. The pan-cancer datasets were used to search for links between CCNE1 expression and prognosis, DNA methylation, m6A level, genetic alterations, CCNE1-related genes, and tumor immunity. We verified that CCNE1 has biological functions in UCEC cell lines using CCK-8, EdU, and Transwell assays.

Results
In patients with different tumor types, a high mRNA expression level of CCNE1 was related to a poor prognosis. Genes related to CCNE1 were connected to the cell cycle, metabolism, and DNA damage repair, according to GO and KEGG enrichment analyses. Genetic alterations of CCNE1, including duplications and deep mutations, have been observed in various cancers. Immune analysis revealed that CCNE1 had a strong correlation with TMB, MSI, neoantigen, and ICP in a variety of tumor types, and this correlation may have an impact on the sensitivity of various cancers to immunotherapy. CCK-8, EdU and Transwell assays suggested that CCNE1 knockdown can suppress UCEC cell proliferation, migration and invasion.

Conclusion
Our study demonstrated that CCNE1 is upregulated in multiple cancers in the TCGA database and may be a promising predictive biomarker for the immunotherapy response in some types of cancers. Moreover, CCNE1 knockdown can suppress the proliferation, migration and invasion of UCEC cells.
Intrathecal bupivacaine with clonidine or dexmedetomidine as adjuvant in gynecological surgery: an enigma
Authors: Choudhary Rupesh , Pathania Jyoti , Sharma Anoop , Sharma Aparna , Sharma Rahul 
Published: March 2023

Abstract
Background
Addition of adjuvants to routinely used intrathecal drugs is cornerstone in safe and effective prolongation of single shot spinal block for gynecological surgery. In resource deficient countries, where epidural anesthesia is usually not used because of cost factor, adjuvants are routinely used to prolong the effect of regional anesthesia. Alpha 2 agonists are considered best drugs as adjuvants, but there is inconclusive data in literature about the block characteristic, dose at which to be used and side effect after use of these drugs.

Methods
Clonidine 30 µgm or dexmedetomidine 5 µgm was used intrathecally as an adjuvant with 15 mg bupivacaine 0.5% in 90 female patients undergoing gynecological surgery in this randomized, prospective, single blind study.

Results
The mean time to onset of sensory block a (T6 level) and time to attain maximum sensory height were significantly early in group D over group C (124.44 ± 20.64 s, 175.09 ± 68.01 s, p < 0.0001) and (13.53 ± 2.97 min, 18.64 ± 4.82 min, p < 0.0001)respectively. Time to two segment sensory regression, total duration of analgesia, duration of motor blockade was (115.24 ± 8.9 min, 370.60 ± 17.98 min, 316.67 ± 21.39 min) in group D and (103.58 ± 11.25 min, 323.91 ± 23 min, 273.51 ± 18.95 min) in group C respectively (p < 0.001). The post-operative visual analogue scale score (VAS) was more in group C at 240 min onwards (p ≤ 0.01). Analgesic use and intraoperative complications were similar in both the groups. (p > 0.05).

Conclusions
We recommend clonidine 30 µg over dexmedetomidine 5 µg as an adjuvant to intrathecal bupivacaine, to effectively and safely prolong the effect of single shot spinal anesthesia.
Effect of high body mass index on postoperative pulmonary complications: a retrospective study
Authors: Shiramoto Kiyotaka , Wakamatsu Hiroya , Kametani Yusuke , Matsumoto Satoshi , Ota Keisuke , Morioka Tomoyuki , Harada Kaori , Matsumoto Mishiya 
Published: March 2023

Abstract
Background
Postoperative pulmonary complications (PPCs) are associated with perioperative mortality and morbidity. Obesity physiologically affects respiratory function and thus could be a risk factor for PPCs. The aim of this study was to clarify the effect of high body mass index (BMI) and other factors on the development of PPCs and the perioperative course.

Methods
This study retrospectively analyzed patients admitted to the intensive care unit (ICU) after undergoing elective upper abdominal surgery at our hospital between December 2015 and December 2018. The primary endpoint was the effect of BMI on the development of PPCs, and the secondary endpoints were the effect of BMI on length of ICU stay, ICU readmission, length of hospital stay, and death within 30 days of surgery.

Results
A total of 231 patients were included in this study, 27 of whom had PPCs. BMI was not significantly associated with the development of PPCs. BMI was not significantly associated with length of ICU stay or ICU readmission, whereas higher BMI was significantly associated with shorter hospital stays. The only death within 30 days of surgery occurred in a patient who developed PPCs and died while in the ICU.

Conclusions
We retrospectively investigated the effect of BMI on the development of PPCs, length of ICU stay, and ICU readmission in patients who underwent upper abdominal surgery. BMI was not significantly associated with the development of PPCs, length of ICU stay, or ICU readmission.
On-chip optical sources of 3D photonic integration based on active fluorescent polymer waveguide microdisks for light display application
Authors: Wang Chunxue , Zhang Daming , Yue Jian , Lin Hang , Zhang Xucheng , Zhang Tong , Chen Changming , Fei Teng 
Published: March 2023

AbstractIn this work, on-chip three-dimensional (3D) photonic integrated optical sources based on active fluorescent polymer waveguide microdisks are proposed for light display application. Fluorescent green and red oligomers with high-efficiency photoluminescence are doped into epoxy crosslinking SU-8 polymer as the waveguide gain medium. The microdisk-based on-chip optically pumping light sources are designed and fabricated using the organic functionalized materials by direct UV written process. The promising stacking dual-microdisk structures with double gain layers could provide white signal light source generated perpendicular to the chip, and green signal light source stimulated in the chip. The approach could realize the monolithically on-chip assembled vertical and horizontal bright emitters. The optical pumping threshold power is obtained as 50 mW with continuous-wave (CW) pumping. The average gain coefficient of a white light source is measured by vertical fiber coupling as 112 dB/W, and that of green light source by horizontal fiber coupling as 137 dB/W, respectively. The rising and falling response time of the on-chip optical sources are 60 and 80 µs under modulating pulsed pumping. This technique is very promising for achieving 3D integrated light display application, including photonic circuits and optical information encryption.
Intestinal Vitamin D Receptor Is Dispensable for Maintaining Adult Bone Mass in Mice With Adequate Calcium Intake
Authors: Jiang Heng , Chanpaisaeng Krittikan , Christakos Sylvia , Fleet James C 
Published: March 2023

Abstract
1,25-Dihydroxyvitamin D3 (1,25(OH)2D3)-mediated intestinal calcium (Ca) absorption supplies Ca for proper bone mineralization during growth. We tested whether vitamin D receptor (VDR)-mediated 1,25(OH)2D3 signaling is critical for adult Ca absorption and bone by using mice with inducible Vdr gene knockout in the whole intestine (villin-CreERT2+/− × Vdrf/f, WIK) or in the large intestine (Cdx2-CreERT2+/− ×Vdrf/f, LIK). At 4-month-old, Vdr alleles were recombined (0.05 mg tamoxifen/g BW, intraperitoneally [i.p.], 5 days) and mice were fed diets with either 0.5% (adequate) or 0.2% (low) Ca. Ca absorption was examined after 2 weeks while serum 1,25(OH)2D3, bone mass, and bone microarchitecture were examined after 16 weeks. Intestinal and renal gene expression was measured at both time points (n = 12/genotype/diet/time point). On the 0.5% Ca diet, all phenotypes in WIK and LIK mice were similar to the controls. Control mice adapted to the 0.2% low-Ca diet by increasing renal Cyp27b1 mRNA (3-fold), serum 1,25(OH)2D3 level (1.9-fold), and Ca absorption in the duodenum (Dd, + 131%) and proximal colon (PCo, + 28.9%), which prevented bone loss. In WIK mice, low-Ca diet increased serum 1,25(OH)2D3 (4.4-fold) but Ca absorption remained unaltered in the Dd and PCo. Consequently, significant bone loss occurred in WIK mice (e.g., cortical thickness, Ct.Th, −33.7%). LIK mice adapted to the low-Ca diet in the Dd but not the PCo, and the effect on bone phenotypes was milder (e.g., Ct.Th, −13.1%). Our data suggest intestinal VDR in adult mice prevents bone loss under low Ca intake but is dispensable under adequate calcium intake.
Sports Participation and Neurological Injuries in Pediatric Patients With Chiari I Malformation
Authors: Wieland Clare M. , Holste Katherine G. , Selzer Bela , Garton Hugh J. L. , Muraszko Karin M. , Maher Cormac O. 
Published: March 2023

BACKGROUND:
Currently, there is no consensus recommendation regarding the safety of sports participation for pediatric patients with Chiari I malformation (CM-I).

OBJECTIVE:
To prospectively survey the treated and untreated patients with CM-I to define the risk of sports-associated neurological injury.

METHODS:
A prospective survey was administered to 744 pediatric patients at one neurosurgery clinic between 2010 and 2021. Data were recorded on demographic information, imaging characteristics, treatment, sports participation, and presence of sports-related neurological injury. Patients with incomplete data were called. Two hundred seven patients completed at least 1 subsequent survey and were prospectively followed (mean 1.6 years).

RESULTS:
Of 744 patients with completed surveys, 462 participated in sports. Sports participants were more likely to be older at presentation (P < .001) and have rounded cerebellar tonsil morphology (P < .001). Seasons of sports played before and after CM-I decompression (CMD) totaled 5918.7 and 936, respectively. There were 84 sports-related concussions among 55 patients; 79 in untreated patients and 5 after CMD. For all sports participants, the concussion rate was 12.3/1000 seasons of all sports, 9.2/1000 seasons of limited-contact sports, and 13.8/1000 of contact sports. The concussion rate after CMD was 5.3/1000 seasons of all sports, 9.2/1000 seasons of limited-contact sports, and 7.1/1000 seasons of contact sports. There were no reports of long-lasting neurological issues postconcussion or of permanent spinal cord injury.

CONCLUSION:
No permanent or catastrophic sports-associated neurological injuries were reported. The concussion rates in treated and untreated patients with CM-I were low. Therefore, sports participation in this population should be permitted in most cases.
Effect of topical Zingiber cassumunar on painful diabetic neuropathy: a double-blind randomized-controlled trial
Authors: Jatuten Nachapol , Piyakunmala Phuangthong , Budkaew Jiratha , Chumworathayi Bandit 
Published: March 2023

Background: Plai or Zingiber cassumunar Roxb. was registered into the Thai Traditional Medicine list since 2011. However, there is limited evidence regarding Plai as a treatment in painful diabetic neuropathy (PDN). Therefore, this study aimed to evaluate the efficacy of topical Zingiber cassumunar. Methods: A RCT was conducted in patients with PDN during February to March 2019. All participants received oral gabapentin 300 mg before bed as a standard regimen. The intervention group (n=16) received Plai balm 15%w/w 0.5 gram to apply on their feet three times a day and the control group (n=15) received placebo balm to similarly apply. Pain score at baseline, 2nd and 4th weeks were assessed and compared. Patients’ quality of life, and adverse events, were collected. Mean pain scores before and after treatment in each group and between groups were also analyzed. Results: At the end of week two and week four, the Plai group showed statistically significant lesser mean pain scores than the placebo group by -1.47 (95%CI: -1.96 to -1.30, p-value < 0.001), and by -1.51 (95%CI: -1.92 to -0.13, p-value = 0.027), respectively. Moreover, the Plai group had more cases number/ percentages with at least 50% pain score reduction than the placebo group [12/16 (75%) vs 3/15 (20%), p-value = 0.004]. However, there was no statistically significant difference in quality of life between the two groups (overall p-value = 0.366). Adverse event was not found in any groups. Conclusions: Zingiber cassumunar balm (Plai) was efficacious for pain reduction in painful diabetic neuropathy. Registration: Registered with the Thai Clinical Trials Registry; TCTR20200221001.
Safety of a 2-dose primary series of 13-valent pneumococcal conjugate vaccine in Indonesian infants
Authors: Sundoro Julitasari , Prayitno Ari , Satari Hindra Irawan , Djelantik I Gusti Gede , Fletcher Mark Andrew , Hadinegoro Sri Rezeki , Syafriyal  
Published: March 2023

BACKGROUND In 2017, the Indonesian Technical Advisory Group on Immunization recommended a safety monitoring demonstration program for the 13-valent pneumococcal conjugate vaccine (PCV13) in West Lombok and East Lombok, West Nusa Tenggara to evaluate the 2-dose primary series (2 and 3 months of age) for serious adverse events (SAEs), adverse events, systemic events, and local reactions.
METHODS A total of 1,083 infants from 10 primary healthcare centers were analyzed, with 687 receiving the first dose and 396 receiving the second dose. Based on the national immunization program, they received PCV13 + DTwP-HB-Hib + OPV (n = 544), PCV13 + DTwP-HB-Hib (n = 101), or PCV13 only (n = 403). They were monitored for 30 min after vaccination for any immediate SAEs, and parents were given a diary card to record safety information prospectively for 28 days.
RESULTS No immediate SAEs were observed, and no SAEs were reported during 28 days after vaccination. Reports of local reactions and systemic events predominated on days 1–3 post-vaccination. Severe fever (axillary temperature >39.0°C) was uncommon (<2% of all infants). Most irritability was mild to moderate. Local pain was more frequent after the first dose than after the second dose. It was distributed evenly across mild, moderate, and severe classifications, while redness and swelling were mostly mild to moderate.
CONCLUSIONS The PCV13 primary series demonstration program in Indonesia confirmed tolerable local and systemic reactions.