bluuebunny/crossref_metadata_2025_split huggingface dataset:
Development of alcohol vinegars macerated with ACAI (Euterpe precatoria Mart.) berries and their quality evaluations with emphasis on color, antioxidant capacity, and volatiles profile
Authors: Boeira Lúcia S. , Cád Sandra V. , Bezerra Jaqueline A. , Benavente César T. , Neta Maria Terezinha S. L. , Sandes Rafael Donizete Dutra , Narain Narendra 
Published: January 2023

AbstractIn this work, the Amazonian native acai fruit, a superfruit recognized worldwide, was used through a simple operation of maceration in alcohol vinegar to transform it into an attractive and functional product containing the acai natural colorant and its bioactive compounds. The variables studied were the proportion of alcohol vinegar to acai (8:2 and 1:1) and maceration period (7, 14, and 21 days). The final vinegar was subjected to the determination of color parameters, antioxidant capacity (DPPH, ABTS), total phenolics content (TPC), volatile compounds extracted by stir bar sorptive extraction and identified by gas‐chromatography–mass spectrometry analysis. The alcohol vinegars macerated with acai presented the color according to the content of acai added and maceration period employed, whereas antioxidant capacity and TPC were comparable to vinegars elaborated from fruits and red wine. Sixty volatiles compounds classified into five chemical groups were identified. The principal volatile compounds which contributed to the aroma in the products were 3‐methyl‐1‐butanol, phenylethyl alcohol, benzaldehyde, o‐cymene, p‐cymenene, isoamyl acetate, and ethyl acetate. The most attractive product regarding the parameters studied was obtained from the use of the proportion of 1:1 of alcohol vinegar:acai and maceration period of 14 days. This product retained the most similar color to acai in natura, the highest values for antioxidant capacity measured by ABTS and TPC while being rich in volatile compounds due to the contributions mainly of alcohols, esters, aldehydes, and terpenes.Practical ApplicationThis work demonstrates the feasibility to produce an alcohol vinegar with an attractive color and functional properties by the addition of acai resulting in to a wide spectrum of chemical compounds of acai through a very simple operation of maceration during 14 days of a proportion of 1:1 of alcohol vinegar:acai.
Left–Right‐Position, party affiliation and regional differences explain low COVID‐19 vaccination rates in Germany
Authors: Jäckle Sebastian , Timmis James K. 
Published: January 2023

AbstractEstablished vaccine hesitancy measurement instruments, such as the Vaccine Hesitancy Determinants Matrix, are not sufficiently equipped to adequately and consistently measure political and ideological attitudes. Focusing on Germany, which is a particularly interesting case since it witnessed the establishment of the by far most well‐organized and sustained ‘anti‐Covid’ movement in Europe, this quantitative study explores the impact of political ideology and partisanship on the degree of vaccine hesitancy based on four surveys (February—October 2021) among more than 30,000 individuals. We demonstrate that party affiliation, political ideology and region of residence all impact vaccine hesitancy. In fact, they turn out to have a greater impact than two factors often analysed with respect to vaccine hesitancy: gender and educational background. Further interaction models show that the effect of political ideology on vaccine hesitancy is moderated by age, gender and region of residency. For instance, while the more rightwing a young individual is, the more hesitant they are towards SARS‐CoV‐2 vaccination—for older individuals, this is not the case. Our findings are relevant for future investigators measuring vaccine hesitancy and policy makers contemplating the differential impact of complex public health interventions: as the impact of political and ideological attitudes on vaccine hesitancy are not adequately captured by established vaccine hesitancy measurement instruments, we recommend its modification to include a clear and harmonised definition of the political‐ideological dimension of vaccine hesitancy together with pre‐validated measurement items that improve future studies. In addition, we reason that vaccine hesitancy, while being an outcome of complex socio‐political factors, is in itself an indicator for societal cohesion and anomie, the degree of which is associated with trust in (health) policy makers, (public) health authorities, health service providers, etc. Therefore, we further recommend that vaccine hesitancy questions should be integrated in pertinent national surveys.
FOXO3A‐short is a novel regulator of non‐oxidative glucose metabolism associated with human longevity
Authors: Santo Evan E. , Ribel‐Madsen Rasmus , Stroeken Peter J. , de Boer Vincent C. J. , Hansen Ninna S. , Commandeur Maaike , Vaag Allan A. , Versteeg Rogier , Paik Jihye , Westerhout Ellen M. 
Published: January 2023

AbstractIntronic single‐nucleotide polymorphisms (SNPs) in FOXO3A are associated with human longevity. Currently, it is unclear how these SNPs alter FOXO3A functionality and human physiology, thereby influencing lifespan. Here, we identify a primate‐specific FOXO3A transcriptional isoform, FOXO3A‐Short (FOXO3A‐S), encoding a major longevity‐associated SNP, rs9400239 (C or T), within its 5′ untranslated region. The FOXO3A‐S mRNA is highly expressed in the skeletal muscle and has very limited expression in other tissues. We find that the rs9400239 variant influences the stability and functionality of the primarily nuclear protein(s) encoded by the FOXO3A‐S mRNA. Assessment of the relationship between the FOXO3A‐S polymorphism and peripheral glucose clearance during insulin infusion (Rd clamp) in a cohort of Danish twins revealed that longevity T‐allele carriers have markedly faster peripheral glucose clearance rates than normal lifespan C‐allele carriers. In vitro experiments in human myotube cultures utilizing overexpression of each allele showed that the C‐allele represses glycolysis independently of PI3K signaling, while overexpression of the T‐allele represses glycolysis only in a PI3K‐inactive background. Supporting this finding inducible knockdown of the FOXO3A‐S C‐allele in cultured myotubes increases the glycolytic rate. We conclude that the rs9400239 polymorphism acts as a molecular switch which changes the identity of the FOXO3A‐S‐derived protein(s), which in turn alters the relationship between FOXO3A‐S and insulin/PI3K signaling and glycolytic flux in the skeletal muscle. This critical difference endows carriers of the FOXO3A‐S T‐allele with consistently higher insulin‐stimulated peripheral glucose clearance rates, which may contribute to their longer and healthier lifespans.
Notch activation shifts the fate decision of senescent progenitors toward myofibrogenesis in human adipose tissue
Authors: Boulet Nathalie , Briot Anaïs , Jargaud Valentin , Estève David , Rémaury Anne , Belles Chloé , Viana Pénélope , Fontaine Jessica , Murphy Lucie , Déon Catherine , Guillemot Marie , Pech Catherine , Veeranagouda Yaligara , Didier Michel , Decaunes Pauline , Mouisel Etienne , Carpéné Christian , Iacovoni Jason S. , Zakaroff‐Girard Alexia , Grolleau Jean‐Louis , Galitzky Jean , Ledoux Séverine , Guillemot Jean‐Claude , Bouloumié Anne 
Published: January 2023

AbstractSenescence is a key event in the impairment of adipose tissue (AT) function with obesity and aging but the underlying molecular and cellular players remain to be fully defined, particularly with respect to the human AT progenitors. We have found distinct profiles of senescent progenitors based on AT location between stroma from visceral versus subcutaneous AT. In addition to flow cytometry, we characterized the location differences with transcriptomic and proteomic approaches, uncovering the genes and developmental pathways that are underlying replicative senescence. We identified key components to include INBHA as well as SFRP4 and GREM1, antagonists for the WNT and BMP pathways, in the senescence‐associated secretory phenotype and NOTCH3 in the senescence‐associated intrinsic phenotype. Notch activation in AT progenitors inhibits adipogenesis and promotes myofibrogenesis independently of TGFβ. In addition, we demonstrate that NOTCH3 is enriched in the premyofibroblast progenitor subset, which preferentially accumulates in the visceral AT of patients with an early obesity trajectory. Herein, we reveal that NOTCH3 plays a role in the balance of progenitor fate determination preferring myofibrogenesis at the expense of adipogenesis. Progenitor NOTCH3 may constitute a tool to monitor replicative senescence and to limit AT dysfunction in obesity and aging.
Renal dysfunction occurs following ileostomy formation and is independent of readmission
Authors: Panizza John , Swee Yan Jing Sherrie , Edmundson Aleks , Clark David 
Published: January 2023

AbstractBackgroundIleostomy formation may be permanent or intended as temporary to defunction a high‐risk pelvic anastomosis to reduce the risk of septic consequences of anastomotic leak. However, these procedures have a high rate of readmission, most commonly due to dehydration and associated with acute kidney injury (AKI).MethodsA single centre retrospective cohort study of patients who underwent ileostomy formation from 2015 to 2020 and analysed in two groups: those who needed readmission within 60 days and those who did not. Data collected included demographics and renal function and electrolytes at baseline (ileostomy formation) and at elective ileostomy closure.ResultsA total of 171 patients were included in the analysis, with a readmission rate 38% within 60 days of discharge. There was a significant increase in creatinine from baseline blood tests compared to date of elective ileostomy closure in both the readmission and no readmission arms. There was a significant decrease in eGFR from baseline blood tests compared with date of ileostomy closure in both readmission and no readmission arms. There was no significant difference in creatinine or eGFR between readmission and no readmission arms at date of ileostomy closure. Baseline serum sodium levels were lower in the readmission arm compared to no readmission arm.ConclusionIleostomy formation is associated with a deterioration in renal function, which occurs independent of whether the patient requires readmission to hospital. Low serum sodium may be used as a predictor for patients with an increased risk of readmission.
Psychological interventions decrease unintended pregnancies: A meta‐analysis of randomized controlled trials
Authors: Swain Le'Sa Tai'Mua , Malouff John M. , Meynadier Jai , Schutte Nicola S. 
Published: January 2023

AbstractObjectiveDespite innovations in contraceptive methods, unintended pregnancies remain common. Researchers have examined psychological approaches to decrease unintended pregnancies through contraceptive use. These interventions have involved applying aspects of social cognitive theory, the health belief model and self‐determination theory. Research findings on the effects of these psychological approaches show conflicting evidence. The aim of this meta‐analysis was to clarify the impact of these psychological interventions on unintended pregnanciesDesignMeta‐analysis of randomized controlled trials (RCTs) of psychological interventions intended to prevent unwanted pregnancies through an increase in the use of contraceptive methodsMethodsA systematic search of databases and article reference lists led to 26 relevant RCTs with a total of 31,222 participantsResultsThe odds ratio for pregnancy in the psychological intervention condition = .83, 95% CI [.75, .93]. The results also showed that the longer an intervention's follow‐up period was, the less the prevention effect. Quality assessment of included studies indicated that all used a treatment manual and reported attrition. It also showed that most studies reported the reasons for drop‐out and assessed the facilitators' adherence to the intervention protocol. The proportion of variability due to chance amongst studies was I2 = 22%. Duval and Tweedie's Trim and Fill showed a difference between the observed and the adjusted values. The adjusted value, representing a conservative estimate of effect size, was OR = .891, 95% CI [.777, .999]ConclusionsAltogether, these results support the efficacy of psychological interventions aimed at preventing unintended pregnancy through contraception.
Oil price shock and informal workers in dual labor markets
Authors: Chaudhuri Sarbajit , Kar Saibal 
Published: January 2023

AbstractThis paper examines the impact of a rise in oil prices on the wages of workers in the unorganized sector of a developing economy. The model economy is comprised of two non‐traded transport sectors, formal and informal, along with other sectors. The main results that we obtain are as follows. The informal transport sector contracts when fuel price rises and lowers the real income of the informal workers. The per‐unit return to land rises, and the factor readjustments even raise the output of other sectors in the economy. We also show why inclusion of non‐passenger transport services does not alter the main outcomes of the model.
Distant metastasis from morphologically low‐grade spiradenocarcinoma: A report of two cases
Authors: Lach Katherine , Gharpuray‐Pandit Deepa , Franko Angela , Brenn Thomas 
Published: January 2023

AbstractMalignant tumors arising from benign eccrine spiradenomas are rare. They are divided by morphology into low‐grade and high‐grade spiradenocarcinomas, with prognosis and metastatic potential closely linked to their histopathologic features. Tumors with low‐grade morphology are known for their indolent behavior, with only two reported instances of metastatic spread. We report herein two further low‐grade metastatic spiradenocarcinomas resulting in distant metastasis. Both tumors showed a background of a benign spiradenoma and subtle histopathologic signs of malignant transformation, characterized by loss of the dual‐cell population, up to moderate cytological atypia and increased mitotic activity. Both patients developed metastases to the lungs years after the initial presentation, and one showed additional lymph nodal disease. We show that even the morphologically low‐grade tumors may rarely show more aggressive behavior. Although often challenging, recognition of the morphologically low‐grade malignant spiradenocarcinoma and long‐term follow‐up of the patients are important to detect metastatic disease.
Global Health Care Experiences of Female Survivors Who Experienced Trafficking: A Meta‐Ethnography
Authors: Vo Timothea , Purkayastha Bandana 
Published: January 2023

IntroductionMost nurses and midwives do not feel adequately prepared to respond to the complex trauma and social and cultural needs of female clients who have experienced trafficking. There are data to support a lack of knowledge among health care providers about the types of human trafficking as well as poor structural supports within health care systems. The purpose of this review was to gain a more comprehensive understanding of the global health care experiences of females who have experienced trafficking.MethodsNoblit and Hare's 7‐step meta‐ethnographic approach was used. We analyzed constructs, concepts, themes, and metaphors using reciprocal translations. The guidelines for preferred reporting the synthesis of qualitative research were adhered to enhancing transparency (Preferred Reporting Items for Systematic Reviews and Meta‐Analyses).ResultsThe collaborative search process in the PsycINFO, CINAHL, and Scopus databases resulted in 7 high‐quality research studies published between January 2016 and January 2021. Most studies explored individuals’ experiences of trafficking and views of health care services, challenges, and service use barriers. A total of 228 female survivors who experienced trafficking participated. They primarily experienced trafficking within different parts of North America, Eastern Europe, Africa, and South and Southeast Asia. Three main themes emerged: privacy, confidentiality, and identity problems; powerlessness; and clinician recognition and responsiveness.DiscussionAll participants reported being exposed to extremely high levels of physical and mental abuse before, during, and after exiting trafficking. Therefore, clinicians will require ongoing training and clearer guidance from clinical leadership to adequately care for the varying health needs of women and girls who have experienced trafficking or are currently in a trafficking situation. Adoption of a culturally sensitive, patient‐centered, and trauma‐informed approach is needed, as clients’ reasons for staying in a trafficking situation vary. Relationships of trust should be formed in which rapport is cocreated and in which members of the health care team and client work hand in hand together to envision, identify, and pursue future‐oriented and strength‐based goals toward healing.
Demography and linked selection interact to shape the genomic landscape of codistributed woodpeckers during the Ice Age
Authors: Moreira Lucas R. , Klicka John , Smith Brian Tilston 
Published: January 2023

AbstractThe influence of genetic drift on population dynamics during Pleistocene glacial cycles is well understood, but the role of selection in shaping patterns of genomic variation during these events is less explored. We resequenced whole genomes to investigate how demography and natural selection interact to generate the genomic landscapes of Downy and Hairy Woodpecker, species codistributed in previously glaciated North America. First, we explored the spatial and temporal patterns of genomic diversity produced by neutral evolution. Next, we tested (i) whether levels of nucleotide diversity along the genome are correlated with intrinsic genomic properties, such as recombination rate and gene density, and (ii) whether different demographic trajectories impacted the efficacy of selection. Our results revealed cycles of bottleneck and expansion, and genetic structure associated with glacial refugia. Nucleotide diversity varied widely along the genome, but this variation was highly correlated between the species, suggesting the presence of conserved genomic features. In both taxa, nucleotide diversity was positively correlated with recombination rate and negatively correlated with gene density, suggesting that linked selection played a role in reducing diversity. Despite strong fluctuations in effective population size, the maintenance of relatively large populations during glaciations may have facilitated selection. Under these conditions, we found evidence that the individual demographic trajectory of populations modulated linked selection, with purifying selection being more efficient in removing deleterious alleles in large populations. These results highlight that while genome‐wide variation reflects the expected signature of demographic change during climatic perturbations, the interaction of multiple processes produces a predictable and highly heterogeneous genomic landscape.
An elite γ‐gliadin allele improves end‐use quality in wheat
Authors: Liu Dan , Yang Huaimao , Zhang Zhaoheng , Chen Qian , Guo Weilong , Rossi Vincenzo , Xin Mingming , Du Jinkun , Hu Zhaorong , Liu Jie , Peng Huiru , Ni Zhongfu , Sun Qixin , Yao Yingyin 
Published: January 2023

Summary

Gluten is composed of glutenins and gliadins and determines the viscoelastic properties of dough and end‐use quality in wheat (Triticum aestivum L.). Gliadins are important for wheat end‐use traits, but the contribution of individual gliadin genes is unclear, since gliadins are encoded by a complex, multigenic family, including many pseudogenes.

We used CRISPR/Cas9‐mediated gene editing and map‐based cloning to investigate the contribution of the γ‐gliadin genes annotated in the wheat cultivar ‘Fielder’, showing that Gli‐γ1‐1D and Gli‐γ2‐1B account for most of the γ‐gliadin accumulation.

The impaired activity of only two γ‐gliadin genes in knockout mutants improved end‐use quality and reduced gluten epitopes associated with celiac disease (CD). Furthermore, we identified an elite haplotype of Gli‐γ1‐1D linked to higher end‐use quality in a wheat germplasm collection and developed a molecular marker for this allele for marker‐assisted selection.

Our findings provide information and tools for biotechnology‐based and classical breeding programs aimed at improving wheat end‐use quality.
The impacts of imports and trade liberalization in Brazil: Insights from an aggregate cost function
Authors: Truett Lila J. , Truett Dale B. 
Published: January 2023

AbstractBrazil has faced and continues to struggle with difficult economic challenges, including achieving noninflationary economic growth and a manageable external deficit. This paper presents the results of the estimation of an aggregate translog cost function with inputs of domestic capital and labor and imports and outputs of consumption and investment goods. The resulting calculated elasticities give insights into the relationships among the inputs, as well as the possible impacts of further reducing international trade barriers. The findings suggest that all of the inputs are substitutes for one another and that an increase in imports may have at least short‐run negative effects on the demand for domestic capital and labor. However, imports appear to be particularly important in the production of investment goods, so an increase in imports may result in a more than proportionate increase in the output of those products and enhance the long‐term growth of the economy. The responses to current challenges facing Brazil will affect its future economic growth as well as have a broader impact on the international scene. The results described here may help to inform policy makers as they address these issues, particularly with respect to international trade policies.
The Effect of Ministerial Instability on Government's Mandate Fulfilment: Evidence from the Portuguese Case
Authors: Belchior Ana Maria , Silveira Pedro 
Published: January 2023

AbstractResearch on the fulfilment of electoral promises has been particularly fruitful over the past decades. Most of it focused on examining pledge fulfilment at the cabinet level, and little emphasis was placed on the reasons underlying the level of compliance. As a consequence, core factors in explaining pledge fulfilment have not yet been explored. One such factor might be instability in a government's internal functioning. We argue that ministerial instability is relevant for explaining a government's broken promises, and that its importance increases at the junior minister level and among the most salient ministries. Relying on data on the fulfilment of electoral promises and ministerial instability in Portugal between 1995 and 2019, backed by interviews with former ministers and junior ministers, we provide evidence that the fulfilment of electoral promises is significantly influenced by portfolio volatility, particularly at the junior ministerial level and in the most important ministries.