bluuebunny/crossref_metadata_2025_split huggingface dataset:
Why should clinical practitioners ask about their patients’ concerns about falling?
Authors: Ellmers Toby Jack , Freiberger Ellen , Hauer Klaus , Hogan David B , McGarrigle Lisa , Lim Mae Ling , Todd Chris , Martin Finbarr , Delbaere Kim ,   The World Falls Guidelines Working Group on Concerns About Falling, Kempen Ruud , sadly Ruud , Martin F 
Published: April 2023

Abstract
Concerns (or ‘fears’) about falling (CaF) are common in older adults. As part of the ‘World Falls Guidelines Working Group on Concerns about Falling’, we recommended that clinicians working in falls prevention services should regularly assess CaF. Here, we expand upon these recommendations and argue that CaF can be both ‘adaptive’ and ‘maladaptive’ with respect to falls risk. On the one hand, high CaF can lead to overly cautious or hypervigilant behaviours that increase the risk of falling, and may also cause undue activity restriction (‘maladaptive CaF’). But concerns can also encourage individuals to make appropriate modifications to their behaviour to maximise safety (‘adaptive CaF’). We discuss this paradox and argue that high CaF—irrespective of whether ‘adaptive’ or ‘maladaptive’—should be considered an indication that ‘something is not right’, and that is represents an opportunity for clinical engagement. We also highlight how CaF can be maladaptive in terms of inappropriately high confidence about one’s balance. We present different routes for clinical intervention based on the types of concerns disclosed.
Heterogeneity of reported outcomes in epidermolysis bullosa clinical research: a scoping review as a first step towards outcome harmonization
Authors: Korte Eva W H , Welponer Tobias , Kottner Jan , van der Werf Sjoukje , van den Akker Peter C , Horváth Barbara , Kiritsi Dimitra , Laimer Martin , Pasmooij Anna M G , Wally Verena , Bolling Maria C 
Published: April 2023

Abstract

Background
Epidermolysis bullosa (EB) is a rare, genetically and clinically heterogeneous group of skin fragility disorders. No cure is currently available, but many novel and repurposed treatments are upcoming. For adequate evaluation and comparison of clinical studies in EB, well-defined and consistent consensus-endorsed outcomes and outcome measurement instruments are necessary.

Objectives
To identify previously reported outcomes in EB clinical research, group these outcomes by outcome domains and areas and summarize respective outcome measurement instruments.

Methods
A systematic literature search was performed in the databases MEDLINE, Embase, Scopus, Cochrane CENTRAL, CINAHL, PsycINFO and trial registries covering the period between January 1991 and September 2021. Studies were included if they evaluated a treatment in a minimum of three patients with EB. Two reviewers independently performed the study selection and data extraction. All identified outcomes and their respective instruments were mapped onto overarching outcome domains. The outcome domains were stratified according to subgroups of EB type, age group, intervention, decade and phase of clinical trial.

Results
The included studies (n = 207) covered a range of study designs and geographical settings. A total of 1280 outcomes were extracted verbatim and inductively mapped onto 80 outcome domains and 14 outcome areas. We found a steady increase in the number of published clinical trials and outcomes reported over the past 30 years. The included studies mainly focused on recessive dystrophic EB (43%). Wound healing was reported most frequently across all studies and referred to as a primary outcome in 31% of trials. Great heterogeneity of reported outcomes was observed within all stratified subgroups. Moreover, a diverse range of outcome measurement instruments (n = 200) was identified.

Conclusions
We show substantial heterogeneity in reported outcomes and outcome measurement instruments in EB clinical research over the past 30 years. This review is the first step towards harmonization of outcomes in EB, which is necessary to expedite the clinical translation of novel treatments for patients with EB.
Blue light and skin: what is the intriguing link?
Authors: Das Anupam , Sil Abheek , Kumar Piyush , Khan Ismat 
Published: April 2023

Abstract
Blue light has garnered attention because of its ability to penetrate more deeply into the skin layers, and induce cellular dysfunction and DNA damage. Photoageing, hyperpigmentation and melasma are some of the cutaneous changes that develop on exposure to blue light. To date, the therapeutic roles of blue light have been evaluated in dermatological conditions like psoriasis, eczema, acne vulgaris, actinic keratosis and cutaneous malignancies, among others. In this review, we have attempted to present an evidence-based compilation of the effects of blue light on the skin.
The effect of immunomodulatory therapy with recombinant human interferon alpha-2β on blood cytokine levels in children with recurrent episodes of acute obstructive bronchitis
Authors: Kenzhetaeva Tattigul , Lukyanenko Nataliia S , Kaliakpar Danara M , Dolinnaya Vilena T , Tkachenko Olexandr 
Published: April 2023

Abstract
The high incidence of children with recurrent episodes of acute obstructive bronchitis is a widespread problem. Correct identification of children at risk of developing bronchial asthma at school age may improve treatment and prevention approaches to this pathology, but the ability to identify these children remains limited. The purpose of the study was to determine the effectiveness of recombinant interferon alpha-2β in children with recurrent episodes of acute obstructive bronchitis in the course of treatment based on the assessment of cytokine profile. The study examined 59 children of the main group with recurrent episodes of acute obstructive bronchitis and 30 children of the comparison group who suffered from acute bronchitis, aged 2–8 years, who were in the hospital. The results of laboratory studies were compared with the data of 30 healthy children. In children with recurrent episodes of acute obstructive bronchitis, the content of serum interferon-γ and interleukin-4 was significantly reduced compared to healthy children, after treatment with recombinant human interferon alpha-2β, the content of interferon-γ and interleukin-4 in children significantly increased. The content of interleukin-1β in children with recurrent episodes of acute obstructive bronchitis was significantly higher than in healthy children, after immunomodulatory therapy with recombinant interferon alpha-2β, interleukin-4 normalized to its level in healthy children. It was found that children with recurrent episodes of acute obstructive bronchitis have an imbalance of cytokines, the effectiveness of recombinant human interferon alpha-2β therapy, which normalized the levels of the studied cytokines in the serum.
A Publicly Verifiable Optimistic Fair Exchange Protocol Using Decentralized CP-ABE
Authors: Zhang Liang , Kan Haibin , Qiu Feiyang , Hao Feng 
Published: April 2023

Abstract
Fair exchange is a challenging problem for two mutually distrusting players. It is widely known that fair exchange is impossible without a trusted third party (TTP). However, relying on a single TTP can cause a single-point failure. An intuitive idea is to adopt multiple TTPs to distribute trust. This paper constructs a two-party optimistic fair exchange (OFE) protocol using decentralized ciphertext-policy attribute-based encryption (CP-ABE), achieving decentralized TTPs. This is achievable because decentralized CP-ABE ciphertext supports a nested access control policy. A nested access control policy fits perfectly in a fair exchange protocol which contains multiple roles (i.e. players and TTPs). Further, we apply non-interactive zero knowledge proofs to prove the well-formedness of ciphertexts, so as to enforce players to follow the protocol specification honestly. Consequently, we construct an OFE protocol in which each player’s operations are publicly verifiable without revealing secret information. Also, we obtain decentralized TTPs with optimism (i.e. the TTPs are involved only when arbitration is required), autonomy (i.e. the TTPs do not need to interact with each other), statelessness (i.e. the TTPs do not need to store data for the exchange protocol) and verifiability (i.e. the TTPs are publicly verifiable). Compared with previous work, our protocol assumes only a public communication channel and each party’s operations are publicly verifiable. Besides, it achieves a favorable $O(n)$ verification complexity in the normal case, where $n$ is the number of TTPs. Finally, we present a proof-of-concept implementation to demonstrate the feasibility.
An Improved Genetic-XGBoost Classifier for Customer Consumption Behavior Prediction
Authors: Li Yue , Qi Jianfang , Jin Haibin , Tian Dong , Mu Weisong , Feng Jianying 
Published: April 2023

AbstractIn an increasingly competitive market, predicting the customer’s consumption behavior has a vital role in customer relationship management. In this study, a new classifier for customer consumption behavior prediction is proposed. The proposed methods are as follows: (i) A feature selection method based on least absolute shrinkage and selection operator (Lasso) and Principal Component Analysis (PCA), to achieve efficient feature selection and eliminate correlations between variables. (ii) An improved genetic-eXtreme Gradient Boosting (XGBoost) for customer consumption behavior prediction, to improve the accuracy of prediction. Furthermore, the global search ability and flexibility of the genetic mechanism are used to optimize the XGBoost parameters, which avoids inaccurate parameter settings by manual experience. The adaptive crossover and mutation probabilities are designed to prevent the population from falling into the local extremum. Moreover, the grape-customer consumption behavior dataset is employed to compare the six Lasso-based models from the original, normalized and standardized data sources with the Isometric Mapping, Locally Linear Embedding, Multidimensional Scaling, PCA and Kernel Principal Component Analysis methods. The improved genetic-XGBoost is compared with several well-known parameter optimization algorithms and state-of-the-art classification approaches. Furthermore, experiments are conducted on the University of California Irvine datasets to verify the improved genetic-XGBoost algorithm. All results show that the proposed methods outperform the existing ones. The prediction results provide the decision-making basis for enterprises to formulate better marketing strategies.
Review of databases for experimentally validated human microRNA–mRNA interactions
Authors: Kariuki Dorian , Asam Kesava , Aouizerat Bradley E , Lewis Kimberly A , Florez Jose C , Flowers Elena 
Published: April 2023

Abstract
MicroRNAs (miRs) may contribute to disease etiology by influencing gene expression. Numerous databases are available for miR target prediction and validation, but their functionality is varied, and outputs are not standardized. The purpose of this review is to identify and describe databases for cataloging validated miR targets. Using Tools4miRs and PubMed, we identified databases with experimentally validated targets, human data, and a focus on miR–messenger RNA (mRNA) interactions. Data were extracted about the number of times each database was cited, the number of miRs, the target genes, the interactions per database, experimental methodology and key features of each database. The search yielded 10 databases, which in order of most cited to least were: miRTarBase, starBase/The Encyclopedia of RNA Interactomes, DIANA-TarBase, miRWalk, miRecords, miRGator, miRSystem, miRGate, miRSel and targetHub. Findings from this review suggest that the information presented within miR target validation databases can be enhanced by adding features such as flexibility in performing queries in multiple ways, downloadable data, ongoing updates and integrating tools for further miR–mRNA target interaction analysis. This review is designed to aid researchers, especially those new to miR bioinformatics tools, in database selection and to offer considerations for future development and upkeep of validation tools.
Database URL http://mirtarbase.cuhk.edu.cn/
Pulmonary artery pulsatility index in patients with tricuspid valve regurgitation: a simple non-invasive tool for risk stratification
Authors: Kane Conor J , Lara-Breitinger Kyla M , Alabdaljabar Mohamad S , Nkomo Vuyisile T , Padang Ratnasari , Pislaru Cristina , Kane Garvan C , Scott Christopher , Pislaru Sorin V , Lin Grace 
Published: April 2023

Abstract

Aims
Tricuspid valve regurgitation (TR) is a common valvular disease associated with increased mortality. There is a need for tools to assess the interaction between the pulmonary artery (PA) circulation and the right ventricle in patients with TR and to investigate their association with outcomes. The pulmonary artery pulsatility index (PAPi) has emerged as a haemodynamic risk predictor in left heart disease and pulmonary hypertension (PH). Whether PAPi discriminates risk in unselected patients with greater than or equal to moderate TR is unknown.

Methods and results
In 5079 patients with greater than or equal to moderate TR (regardless of aetiology) and PA systolic and diastolic pressures measured on their first echocardiogram, we compared all-cause mortality at 5 years based on the presence or absence of PH and PAPi levels. A total of 2741 (54%) patients had PH. The median PAPi was 3.0 (IQR 1.9, 4.4). Both the presence of PH and decreasing levels of PAPi were associated with larger right ventricles, worse right ventricular systolic function, higher NT-pro BNP levels, greater degrees of right heart failure, and worse survival. In a subset of patients who had an echo and right heart catheterization within 24 h, the correlation of non-invasive to invasive PA pressures and PAPi levels was very good (r = 0.76).

Conclusion
In patients with greater than or equal to moderate TR with and without PH, lower PAPi is associated with right ventricular dysfunction, right heart failure, and worse survival. Incorporating PA pressure and PAPi may help stratify disease severity in patients with greater than or equal to moderate TR regardless of aetiology.
Ablation of atrial fibrillation and dementia risk reduction during long-term follow-up: a nationwide population-based study
Authors: Li Guan-Yi , Chen Yun-Yu , Lin Yenn-Jiang , Chien Kuo-Liong , Hsieh Yu-Cheng , Chung Fa-Po , Lo Li-Wei , Chang Shih-Lin , Chao Tze-Fan , Hu Yu-Feng , Lin Chin-Yu , Chen Shih-Ann 
Published: April 2023

Abstract

Aims
This study investigated the epidemiological characteristics of new-onset dementia in patients with atrial fibrillation (AF) and the association of catheter ablation with different subtypes of dementia.

Methods and results
We conducted a population-based, retrospective cohort study using data from the Taiwan National Health Insurance Research Database. In total, 136 774 patients without a history of dementia were selected after 1:1 propensity score matching based on age (with AF vs. without AF). A competing risk model was used to investigate the three subtypes of dementia: Alzheimer’s disease, vascular dementia, and other/mixed dementia. Inverse probability of treatment weighting (IPTW) was performed to minimize the impact on dementia risk due to the imbalanced baseline characteristics. After a median follow-up period of 6.6 years, 8704 events of new-onset dementia occurred. Among all AF patients developing dementia, 73% were classified as having Alzheimer’s disease, 16% as having vascular dementia, and 11% as having other/mixed dementia. The cumulative incidence of dementia in AF patients was higher than those without AF (log-rank test: P &lt; 0.001 for both before and after IPTW). In patients with AF undergoing catheter ablation, the total dementia risk decreased significantly [P = 0.015, hazard ratio (HR): 0.74, 95% confidence interval (CI): 0.58–0.94] after multivariable adjustment, but not for the subtype of vascular dementia (P = 0.59, HR: 0.86, 95% CI: 0.49–1.50).

Conclusion
Patients with AF have a higher incidence of all types of dementia, including Alzheimer’s disease, vascular dementia, and a mixed type of dementia. Alzheimer’s disease is less likely to occur in patients with AF undergoing catheter ablation.
Educational inequalities in hypothermia mortality in the Baltic countries and Finland in 2000–15
Authors: Stickley Andrew , Baburin Aleksei , Jasilionis Domantas , Krumins Juris , Martikainen Pekka , Kondo Naoki , Shin Jae Il , Oh Hans , Waldman Kyle , Leinsalu Mall 
Published: April 2023

Abstract

Background
Despite an increased focus on cold-related mortality in recent years, there has been comparatively little research specifically on hypothermia mortality and its associated factors.

Methods
Educational inequalities in hypothermia mortality among individuals aged 30–74 in the Baltic countries (Estonia, Latvia, Lithuania) and Finland in 2000–15 were examined using data from longitudinal mortality follow-up studies of population censuses (the Baltics) and from a longitudinal register-based population data file (Finland).

Results
Age-standardized mortality rates (ASMRs) were much higher in the Baltic countries than in Finland across the study period. From 2000–07 to 2008–15, overall ASMRs declined in all countries except among Finnish women. Although a strong educational gradient was observed in hypothermia mortality in all countries in 2000–07, inequalities were larger in the Baltic countries. Between 2000–07 and 2008–15, ASMRs declined in all educational groups except for high-educated women in Finland and low-educated women in Lithuania; the changes however were not always statistically significant. The absolute mortality decline was often larger among the low educated resulting in narrowing absolute inequalities (excepting Lithuania), whereas a larger relative decline among the high educated (excepting Finnish women) resulted in a considerable widening of relative inequalities in hypothermia mortality by 2008–15.

Conclusion
Although some reduction was observed in absolute educational inequalities in hypothermia mortality in 2000–15, substantial and widening relative inequalities highlight the need for further action in combatting factors behind deaths from excessive cold in socioeconomically disadvantaged groups, including risky alcohol consumption and homelessness.
Is cooperation favored by horizontal gene transfer?
Authors: Scott Thomas W , West Stuart A , Dewar Anna E , Wild Geoff 
Published: April 2023

Abstract
It has been hypothesized that horizontal gene transfer on plasmids can facilitate the evolution of cooperation, by allowing genes to jump between bacteria, and hence increase genetic relatedness at the cooperative loci. However, we show theoretically that horizontal gene transfer only appreciably increases relatedness when plasmids are rare, where there are many plasmid-free cells available to infect (many opportunities for horizontal gene transfer). In contrast, when plasmids are common, there are few opportunities for horizontal gene transfer, meaning relatedness is not appreciably increased, and so cooperation is not favored. Plasmids, therefore, evolve to be rare and cooperative, or common and noncooperative, meaning plasmid frequency and cooperativeness are never simultaneously high. The overall level of plasmid-mediated cooperation, given by the product of plasmid frequency and cooperativeness, is therefore consistently negligible or low.
Bacillus thuringiensis: a natural endophytic bacterium found in wild plants
Authors: Espinoza-Vergara Gabriela , García-Suárez Rosalina , Verduzco-Rosas Luis Alfonso , Cando-Narvaez Areli , Ibarra Jorge E 
Published: April 2023

Abstract
Despite the fact that Bacillus thuringiensis is the most widely used bacterium in biological pest control, its ecology has been notoriously neglected. Its role in nature is uncertain, and a defined habitat and niche are under discussion. In this report, wild-type strains were isolated from the inner plant tissues as natural endophytic bacteria in wild plants. Once a reliable superficial sterilization technique was standardized, leaf samples from 110 wildlife plant species within 52 families were processed to obtain their endophytic microflora, which were able to grow in artificial media. From 93 morphologically different isolates, 22 showed the typical sporangium morphology of B. thuringiensis (endospore and parasporal bodies). These isolates were identified and characterized by their 16S ribosomal RNA, hag gene, MLST, and cry gene sequences. Also, isolates were characterized by Bc-RepPCR and parasporal body protein content. All the isolates showed at least some of the typical B. thuringiensis features tested, but 10 showed information in all those features, which, in a rigorous selection, were taken as B. thuringiensis sensu stricto strains. Only three subspecies were identified: five kurstaki, four nigeriensis, and one thuringiensis. None showed toxicity against mosquito larvae or Caenorhabditis elegans, and only one showed significant toxicity against Manduca sexta larvae. The role of B. thuringiensis as a natural endophytic bacterium is discussed.
The mysterious diadenosine tetraphosphate (AP4A)
Authors: Zegarra Victor , Mais Christopher-Nils , Freitag Johannes , Bange Gert 
Published: April 2023

Abstract
Dinucleoside polyphosphates, a class of nucleotides found amongst all the Trees of Life, have been gathering a lot of attention in the past decades due to their putative role as cellular alarmones. In particular, diadenosine tetraphosphate (AP4A) has been widely studied in bacteria facing various environmental challenges and has been proposed to be important for ensuring cellular survivability through harsh conditions. Here, we discuss the current understanding of AP4A synthesis and degradation, protein targets, their molecular structure where possible, and insights into the molecular mechanisms of AP4A action and its physiological consequences. Lastly, we will briefly touch on what is known with regards to AP4A beyond the bacterial kingdom, given its increasing appearance in the eukaryotic world. Altogether, the notion that AP4A is a conserved second messenger in organisms ranging from bacteria to humans and is able to signal and modulate cellular stress regulation seems promising.
Engineering lanthipeptides by introducing a large variety of RiPP modifications to obtain new-to-nature bioactive peptides
Authors: Fu Yuxin , Xu Yanli , Ruijne Fleur , Kuipers Oscar P 
Published: April 2023

Abstract
Natural bioactive peptide discovery is a challenging and time-consuming process. However, advances in synthetic biology are providing promising new avenues in peptide engineering that allow for the design and production of a large variety of new-to-nature peptides with enhanced or new bioactivities, using known peptides as templates. Lanthipeptides are ribosomally synthesized and post-translationally modified peptides (RiPPs). The modularity of post-translational modification (PTM) enzymes and ribosomal biosynthesis inherent to lanthipeptides enables their engineering and screening in a high-throughput manner. The field of RiPPs research is rapidly evolving, with many novel PTMs and their associated modification enzymes being identified and characterized. The modularity presented by these diverse and promiscuous modification enzymes has made them promising tools for further in vivo engineering of lanthipeptides, allowing for the diversification of their structures and activities. In this review, we explore the diverse modifications occurring in RiPPs and discuss the potential applications and feasibility of combining various modification enzymes for lanthipeptide engineering. We highlight the prospect of lanthipeptide- and RiPP-engineering to produce and screen novel peptides, including mimics of potent non-ribosomally produced antimicrobial peptides (NRPs) such as daptomycin, vancomycin, and teixobactin, which offer high therapeutic potential.