bluuebunny/crossref_metadata_2025_split huggingface dataset:
Tppp3+ synovial/tendon sheath progenitor cells contribute to heterotopic bone after trauma
Authors: Yea Ji-Hye , Gomez-Salazar Mario , Onggo Sharon , Li Zhao , Thottappillil Neelima , Cherief Masnsen , Negri Stefano , Xing Xin , Qin Qizhi , Tower Robert Joel , Fan Chen-Ming , Levi Benjamin , James Aaron W. 
Published: July 2023

AbstractHeterotopic ossification (HO) is a pathological process resulting in aberrant bone formation and often involves synovial lined tissues. During this process, mesenchymal progenitor cells undergo endochondral ossification. Nonetheless, the specific cell phenotypes and mechanisms driving this process are not well understood, in part due to the high degree of heterogeneity of the progenitor cells involved. Here, using a combination of lineage tracing and single-cell RNA sequencing (scRNA-seq), we investigated the extent to which synovial/tendon sheath progenitor cells contribute to heterotopic bone formation. For this purpose, Tppp3 (tubulin polymerization-promoting protein family member 3)-inducible reporter mice were used in combination with either Scx (Scleraxis) or Pdgfra (platelet derived growth factor receptor alpha) reporter mice. Both tendon injury- and arthroplasty-induced mouse experimental HO models were utilized. ScRNA-seq of tendon-associated traumatic HO suggested that Tppp3 is an early progenitor cell marker for either tendon or osteochondral cells. Upon HO induction, Tppp3 reporter+ cells expanded in number and partially contributed to cartilage and bone formation in either tendon- or joint-associated HO. In double reporter animals, both Pdgfra+Tppp3+ and Pdgfra+Tppp3- progenitor cells gave rise to HO-associated cartilage. Finally, analysis of human samples showed a substantial population of TPPP3-expressing cells overlapping with osteogenic markers in areas of heterotopic bone. Overall, these data demonstrate that synovial/tendon sheath progenitor cells undergo aberrant osteochondral differentiation and contribute to HO after trauma.
Diabetic cardiomyopathy: Early diagnostic biomarkers, pathogenetic mechanisms, and therapeutic interventions
Authors: Huo Jin-Ling , Feng Qi , Pan Shaokang , Fu Wen-Jia , Liu Zhangsuo , Liu Zhenzhen 
Published: July 2023

AbstractDiabetic cardiomyopathy (DCM) mainly refers to myocardial metabolic dysfunction caused by high glucose, and hyperglycemia is an independent risk factor for cardiac function in the absence of coronary atherosclerosis and hypertension. DCM, which is a severe complication of diabetes, has become the leading cause of heart failure in diabetic patients. The initial symptoms are inconspicuous, and patients gradually exhibit left ventricular dysfunction and eventually develop total heart failure, which brings a great challenge to the early diagnosis of DCM. To date, the underlying pathological mechanisms of DCM are complicated and have not been fully elucidated. Although there are therapeutic strategies available for DCM, the treatment is mainly focused on controlling blood glucose and blood lipids, and there is a lack of effective drugs targeting myocardial injury. Thus, a large percentage of patients with DCM inevitably develop heart failure. Given the neglected initial symptoms, the intricate cellular and molecular mechanisms, and the lack of available drugs, it is necessary to explore early diagnostic biomarkers, further understand the signaling pathways involved in the pathogenesis of DCM, summarize the current therapeutic strategies, and develop new targeted interventions.
A new daily quarter degree sea level anomaly product from CryoSat-2 for ocean science and applications
Authors: Banks Christopher J. , Calafat Francisco Mir , Shaw Andrew G. P. , Snaith Helen M. , Gommenginger Christine P. , Bouffard Jérôme 
Published: July 2023

AbstractThe European Space Agency launched CryoSat-2 as the first European ice mission in 2010. Its advanced altimeter met primary objectives concerned with sea ice thickness and ice sheets. The value of Cryosat-2 data over global oceans was recognised, and operational products were developed via the CryoSat Ocean Processor (COP). The novel orbit of CryoSat-2 results in a denser coverage of sample points compared to other satellite altimeters. The National Oceanography Centre Sea Level Anomaly (NOCSLA) gridded product is based on interpolating Geophysical Ocean Products (GOP) using weights in space and time. GOP represents the highest quality operational ocean data. NOCSLA is a daily, ¼° sea level anomaly product covering non-coastal oceans between [60°N 60°S] and January 2011 to October 2020. The paper presents the methodology and scientific applications of NOCSLA. Oceanographic features observed are compared against products from other missions, including Rossby waves and El Niño signals. Results show good agreement with other products, confirming the value of Cryosat-2 data for ocean science and applications.
Comparison of bacterial diversity in Bactrocera cucurbitae (Coquillett) ovaries and eggs based on 16S rRNA sequencing
Authors: Lixiang Chen , Zhenya Tian , Weihua Ma , Jingjing Wang , Qiaofen Huang , Yongping Zhou , Xuyuan Gao , Hongsong Chen , Zhongshi Zhou 
Published: July 2023

AbstractNext-generation sequencing allows for fine-scale studies of microbial communities. Herein, 16S ribosomal RNA high-throughput sequencing was used to identify, classify, and predict the functions of the bacterial communities in the eggs and ovaries ofBactrocera cucurbitae(Coquillett) (Diptera: Tephritidae), which is a pest that infests a variety of cucurbit fruits at different developmental stages. Taxonomic analyses indicate that bacteria associated withB. cucurbitaerepresent 19 phyla, which were spread across different developmental stages. Specifically, the egg microbiota had a higher alpha diversity than those of microbiota in the primary and mature ovaries. Significant differences were not observed between the primary and mature ovaries in terms of their microbiota’s alpha diversities. Pseudomonadota, Deinococcota, Bacteroidota, Bacillota, and Actinomycetota were the dominant phyla in all three developmental stages ofB. cucurbitae, andPseudomonadaceaeandEnterobacteriaceaewere the most abundant families. Owing to the unique physiological environment of the ovaries, the diversity of their bacterial community was significantly lower than that in the eggs. This study provides new insights into the structure and abundance of the microbiota inB. cucurbitaeat different developmental stages and contributes to forming management strategies for this pest.
Distribution of alpha temperature anisotropy in the slow and fast solar wind: WIND observations and Vlasov theory
Authors: Li Q. H. , Xiang L. , Wu D. J. , Chen L. , Zhao G. Q. , Zhao A. K. , Zhao Y. , Feng H. Q. 
Published: July 2023

Context. Alpha particles in the solar wind frequently show temperature anisotropies, which can be constrained by various kinetic instabilities. However, the detailed constraint mechanism remains unclear, especially in different types of solar wind.
Aims. Using data obtained by the Wind spacecraft between 2005 and 2015, we investigate the distribution of alpha temperature anisotropy and the correlation with background plasma parameters in the slow and fast solar wind.
Methods. We present a comparison between observations of the alpha temperature anisotropy by the Wind spacecraft and theoretical results obtained with the linear Vlasov theory.
Results. The results show that the distribution of the alpha temperature anisotropy depends not only on the parallel temperature ratio of alpha to proton Tα∥/Tp∥ and the proton temperature anisotropy Tp⊥/Tp∥, but also on the drift velocity of alpha to proton vα/vA and the solar wind velocity vsw. In particular, there are clearly different constraining mechanisms on the distribution of alpha temperature anisotropy in the slow and fast solar wind.
Conclusions. The alpha temperature anisotropy instability together with different types of free energy can be effective for regulating the alpha temperature anisotropy in the slow and fast solar wind.
Flexible gamma photon detector based on plastic scintillation optical fibers
Authors: Rodríguez-Razón Uriel , Vera-Tizatl Pamela , Ramírez-Nava Gerardo , Santos-Cuevas Leticia , Chairez Isaac 
Published: July 2023

Abstract
Detecting small-volume tumoral tissues is a central goal within the nuclear imaging disciplines. Early detection of such small tumors may contribute to the suitable treatment of diverse cancers or the identification of metastatic nodules. Furthermore, the application of feasible radionuclides could contribute to the identification of such tumoral tissues. However, characterizing such radiation sources requires detectors that must adapt their forms to obtain a precise identification of the tumor location. This study's primary outcome was a suitable flexible gamma-ray detector capable of measuring the radiation emitted from small-volumetrics. The detector implements scintillating plastic fibers integrated into an avalanche photodiode (APD), which constitute the main elements of the proposed detection system. The proposed system was characterized in terms of the electrical response, as well as the detection characteristics of the radionuclide source. The optical efficiency of the plastic optical fibers and the photons detection settings of the APD served to develop a prototype of a gamma-probe system. This detection device can be specifically oriented to provide a trustable gamma detector of small-volume radiation sources with the corresponding corrections for the scattering and the compensation for attenuation and background factors. The system design included the corresponding electrical conditioning circuit and the signal processing software. This software detected and discriminated the voltage level to characterize the photon energy. A preclinical study represented the distance and angular sensibilities of the proposed detector to the 99m
Tc-RGD (pegylated arginine-glycine-aspartic acid) radiopharmaceutical in a murine model.
The evaluation of the osteopromoting capabilities of composites based on biopolymers and gold/silver nanoparticles doped bioactive glasses on an experimental rat bone defect
Authors: Dreanca Alexandra , Bogdan Sidonia , Popescu Andra , Sand Denisa , Pall Emoke , Astilean Andreea Niculina , Pestean Cosmin , Toma Corina , Marza Sorin , Taulescu Marian , Cenariu Mihai , Sevastre Bogdan , Oana Liviu , Todea Milica , Baia Lucian , Magyari Klara 
Published: July 2023

Abstract
The most important concept behind using bone scaffolds is the biocompatibility of the material to avoid a local inflammatory response and must have the following properties: osteoinduction, osteoconductivity, angiogenesis, and mechanical support for cell growth. Gold nanoparticles/gold and silver nanoparticles -containing bioactive glasses in biopolymer composites have been used to enhance bone regeneration. These composites were tested in vitro on fibroblast and osteoblast cell lines using MTT tests, immunofluorescence, scanning electron microscopy analysis, and in vivo in an experimental bone defect in Sprague-Dawley rats. Both composites promoted adequate biological effects on human fibroblastic BJ (CRL 2522TM) cell lines and human osteoblastic cells isolated from the human patella in terms of cell proliferation, morphology, migration, and attachment. Most importantly, they did not cause cellular apoptosis and necrosis. According to the histological and immunohistochemical results, both composites were osteoinductive and promoted new bone formation at 60 d. Evidence from this study suggests that the small amount of silver content does not influence negatively the in vitro or in vivo results. In addition, we obtained accurate results proving that the existence of apatite layer and proteins on the surface of the recovered composite, supports the validity of in vitro bioactivity research.
Does mean sea level trend mask historical storm surge trend: evidence from tropical cyclones affecting Japan since 1980
Authors: Islam Md. Rezuanul , Duc Le , Sawada Yohei , Satoh Masaki 
Published: July 2023

Abstract
Variability in storminess, storm surge, and mean sea level (MSL) can substantially alter coastal hazards associated with extreme sea levels (ESLs). However, the detection and attribution of the past changes in tropical cyclone (TC) activity and associated storm surges are hampered by the inhomogeneous TC records. In this study, we investigate spatiotemporal changes in storm surge levels in Japan from 1980 to 2019, a period when observational platforms including tide gauges and storm records are highly consistent. We find statistical evidence supporting the increase in surge annual maxima in several places including the bay area of Tokyo since 1980. This rate of change is comparable to that observed for MSL rise over the same period. These findings cast doubt on the current hypothesis underlying the flood adaptation plan, which assumes that future surge extremes will remain the same and only considers MSL changes. We demonstrate that the changes in ESL in the last 40 years cannot be explained by the rise of MSL alone. Rather, the northeastward shifting of TC landfall location along with intensifying and widening of TCs, might have altered the likelihood of ESL, including surge extremes. The substantial influence of these TC meteorological variables on surge levels combined with the rise of MSL, suggests that current coastal planning practices including critical heights for flood defenses might be inadequate in the future.
Pharmacists’ role in supporting the return of over 10,000 preemptive pharmacogenomics results: The Mayo Clinic experience
Authors: Mitaly Serena , Wright Jessica , El Melik Razan , Matey Eric 
Published: July 2023

Abstract

Purpose
To develop a pharmacist-driven, exploratory pharmacogenomics implementation model with the goal of creating a process for pharmacists to interpret pharmacogenomics results from RIGHT 10K Study samples and provide electronic consults to providers.

Summary
A train-the-trainer model program was initiated whereby pharmacogenomics pharmacists developed a documentation template and a quick reference guide as a standard guide to train other pharmacists. Pharmacists completed electronic consults (e-consults) reviewing pharmacogenomics results, with reference to drug-gene interactions, for patients with “semi-urgent” and “clinically actionable” results, defined as those indicating a potential for gene-drug interactions to cause major harm and those indicating a potential for an adverse drug reaction or reduced efficacy, respectively. Outcomes measured included the number of consults over time, number and role of pharmacists involved, average time to complete e-consults over time, and gene-drug pairs for semi-urgent consults per month. A total of 395 pharmacists were trained. The total number of e-consults completed was 2,843: 61 semi-urgent and 2,782 clinically actionable consults. The average time spent per consult was 24 minutes, and the average number of e-consults per pharmacist was 7. CYP2C19-clopidogrel was the most common gene-drug pair targeted in semi-urgent consults.

Conclusion
Pharmacy leaders planning to implement similar pharmacogenomics programs can utilize this data to estimate hiring needs for future pharmacogenomics implementation, while also considering the potential additional cost of developing resources.
Effects of flash-boil shucking on biochemical, sensorial and ultrastructural properties of Yesso Scallop (Mizuhopecten yessoensis) adductor muscle during refrigeration
Authors: Niu Yabin , Dong Shiliang , Wada Nobuyoshi , Wei Huamao , Yamashita Tetsuro , Takaki Koichi , Yuan Chunhong 
Published: July 2023

Abstract
Scallop adductor muscle has great popularity for raw consumption in Japan, but conventionally shucking live scallops with high vitality by knife requires skill and is time-consuming. Flash-boil shucking is a convenient shucking method in sushi restaurants for live scallops. However, the effects of this treatment on the quality of its main edible part, the adductor muscle, are not clear. This study was conducted to investigate the impact of flash-boil shucking on the quality of raw-edible scallop adductor muscle, focusing on flavor-enhancing components and ultrastructure. Thermal treatment could contribute to the formation of flavor-enhancing nucleotides in the external layer of the adductor muscle. Adenosine monophosphate content increased with the boiling time, which significantly increased to 1.47±0.42 µmol/g in the 30 s-boiled group compared with 0.38±0.13 µmol/g in the control group (p&lt;0.05). The rapid adenosine monophosphate accumulation after boiling resulted in a faster accumulation of inosine and hypoxanthine during subsequent refrigeration, which could bring bitterness to aquatic products. Flash-boil shucking had no discernible effect on the proportion of free amino acids relevant to the flavor of scallop adductor muscle, despite the possibility of a reduction in total free amino acids. The external layer of the scallop adductor muscle could be hardened after flash-boil shucking. At the ultrastructural level, muscle fibers tend to aggregate transversely and contract longitudinally depending on the heating time. Flash-boil shucking is ideal for consuming the raw-edible scallop adductor muscle promptly. Flash-boil shucking treatment is recommended for households, restaurants, and small factories for treating scallops with high vitality.
Advances in endobariatrics: past, present, and future
Authors: Shenoy Abhishek , Schulman Allison R 
Published: July 2023

Abstract
The obesity epidemic in the USA and worldwide is well documented and continues to grow. Endoscopic metabolic and bariatric therapies may offer a less invasive approach than surgical intervention. This article will review advances in endobariatrics over the last several decades, addressing the past and current state of bariatric and metabolic endoscopy. Food and Drug Administration-cleared devices and interventions currently under investigation are described including gastric devices, gastric remodeling procedures, small-bowel devices, duodenal ablation, as well as procedures to address weight regain after bariatric surgery. Future studies evaluating gastric and duodenal combination therapy, adjunctive pharmacotherapy, as well as individualized precision-health algorithms are underway.
Volatile compound-mediated plant–plant interactions under stress with the tea plant as a model
Authors: Jin Jieyang , Zhao Mingyue , Jing Tingting , Zhang Mengting , Lu Mengqian , Yu Guomeng , Wang Jingming , Guo Danyang , Pan Yuting , Hoffmann Timothy D , Schwab Wilfried , Song Chuankui 
Published: July 2023

Abstract
Plants respond to environmental stimuli via the release of volatile organic compounds (VOCs), and neighboring plants constantly monitor and respond to these VOCs with great sensitivity and discrimination. This sensing can trigger increased plant fitness and reduce future plant damage through the priming of their own defenses. The defense mechanism in neighboring plants can either be induced by activation of the regulatory or transcriptional machinery, or it can be delayed by the absorption and storage of VOCs for the generation of an appropriate response later. Despite much research, many key questions remain on the role of VOCs in interplant communication and plant fitness. Here we review recent research on the VOCs induced by biotic (i.e. insects and pathogens) and abiotic (i.e. cold, drought, and salt) stresses, and elucidate the biosynthesis of stress-induced VOCs in tea plants. Our focus is on the role of stress-induced VOCs in complex ecological environments. Particularly, the roles of VOCs under abiotic stress are highlighted. Finally, we discuss pertinent questions and future research directions for advancing our understanding of plant interactions via VOCs.
Glial cell response and microthrombosis in aneurysmal subarachnoid hemorrhage patients: An autopsy study
Authors: Koopman Inez , van Dijk Bart J , Zuithoff Nicolaas P A , Sluijs Jacqueline A , van der Kamp Marije J , Baldew Zelonna A V , Frijns Catharina J M , Rinkel Gabriel J E , Hol Elly M , Vergouwen Mervyn D I 
Published: July 2023

Abstract
Neuroinflammation and microthrombosis may be underlying mechanisms of brain injury after aneurysmal subarachnoid hemorrhage (aSAH), but they have not been studied in relation to each other. In postmortem brain tissue, we investigated neuroinflammation by studying the microglial and astrocyte response in the frontal cortex of 11 aSAH and 10 control patients. In a second study, we investigated the correlation between microthrombosis and microglia by studying the microglial surface area around vessels with and without microthrombosis in the frontal cortex and hippocampus of 8 other aSAH patients. In comparison with controls, we found increased numbers of microglia (mean ± SEM 50 ± 8 vs 20 ± 5 per 0.0026 mm³, p &lt; 0.01), an increased surface area (%) of microglia (mean ± SEM 4.2 ± 0.6 vs 2.2 ± 0.4, p &lt; 0.05), a higher intensity of the astrocytic intermediate filament protein glial fibrillary acidic protein (GFAP) (mean ± SEM 184 ± 28 vs 92 ± 23 arbitrary units, p &lt; 0.05), and an increased GFAP surface area (%) (mean ± SEM 21.2 ± 2.6 vs 10.7 ± 2.1, p &lt; 0.01) in aSAH tissue. Microglia surface area was approximately 40% larger around vessels with microthrombosis than those without microthrombosis (estimated marginal means [95% CI]; 6.1 [5.4–6.9] vs 4.3 [3.6–5.0], p &lt; 0.001). Our results show that the microglial and astrocyte surface areas increased after aSAH and that microthrombosis and microglia are interrelated.
Impact of Metagenomic Next-Generation Sequencing of Plasma Cell-free DNA Testing in the Management of Patients With Suspected Infectious Diseases
Authors: Linder Kathleen A , Miceli Marisa H 
Published: July 2023

AbstractMetagenomic next-generation sequencing (mNGS) of cell-free DNA is an emerging modality for the diagnosis of infectious diseases, but studies on its clinical utility are limited. We conducted a retrospective single-center study including all patients who had plasma mNGS sent at the University of Michigan between 1 January 2021 and 25 July 2022. Test results were assessed for clinical impact. A total of 71 tests were sent on 69 patients; the mean ± SD age was 52 ± 19 years; and 35% of patients were immunocompromised. Forty-five (63%) mNGS test results were positive and 14 (31%) had clinical impact—from starting new antimicrobials (n = 7), discontinuing antimicrobials (n = 4), or changing antimicrobial duration (n = 2) or by affecting surgical decision making (n = 1). Twenty-six (37%) mNGS test results were negative and only 4 (15%) were impactful, leading to discontinuation of antimicrobials. Overall, just 25% of mNGS tests were clinically relevant. There was no significant difference in the proportion of tests that were clinically relevant between negative and positive results (P = .16) or if patients were immunocompromised (P = .57). Plasma mNGS was most frequently impactful (in 50% of patients) when included in the diagnostic workup of cardiovascular infection but less impactful in other clinical syndromes, including fever of unknown origin and pulmonary infection. Our findings underscore the need to further study this testing modality, particularly with prospective research including negative controls, before it is considered for widespread use.