bluuebunny/crossref_metadata_2025_split huggingface dataset:
Seroprevalence of Contagious Bovine Mycoplasma mycoides subsp. mycoides small colony type (MmmSC) in Cattle from Karamoja Region, North-eastern Uganda
Authors: Tweyongyere Robert , Nkamwesiga Joseph , Etiang Patrick , Mugezi Israel , Wamala Henry , Wasswa Auther Tamale , Kamusiime Maureen , Ainebyoona Solomon , Abizera Harriet , Mwiine Frank Norbert , Muhanguzi Dennis 
Published: September 2023

Abstract
Background
Contagious bovine pleuropneumonia [CBPP] is a transboundary animal disease of cattle caused by Mycoplasma mycoides subsp. mycoides biotype small colony [MmmSC]. CBPP causes severe economic losses to livestock producers in sub-Saharan Africa mainly due to high mortality, morbidity, reduction in productivity as well as livestock trade restrictions. This study aimed at determining seroprevalence of MmmSC in cattle from Karamoja region, north-eastern Uganda; data that are required to design and implement risk based CBPP control program.
Methods
We randomly collected blood samples from 2,300 cattle spread across Karamoja region. Serum was extracted and screened for antibodies against Mycoplasma mycoides subsp. mycoides biotype small colony [MmmSC] using the competitive enzyme linked immunosorbent assay [cELISA]
Results
A quarter [25.4%; 95% CI: 23.7–27.3] of the screened cattle [n = 2,300] were sero-positive for MmmSC. Amudat and Kaabong districts recorded the lowest [12.3%] and highest [30.7%] MmmSC seroprevalence respectively. Increasing age, overnight stay in cattle kraals and location [certain districts, villages, herds and sub counties] of the cattle herds, the factors that promote animal commingling, were the most significant risk factors of seroconversion with MmmSC.
Conclusion
Results from this study indicated a higher seroprevalence of MmmSC in Karamoja region cattle herds than previously reported implying that CBPP endemicity has increased over time. To be effective, CBPP vaccination programs should target high risk herds along the international borders and other hotspot areas [e.g., parishes or sub counties]. Additionally, animals should be vaccinated against CBPP prior to being considered for overnight stays in protected kraals.
Evaluation of colorectal cancer in ulcerative colitis reveals key immune factors during its malignant transformation
Authors: Ma Jiao , Wang Qing , Wang Chaoye , Liu Chuwei , Yu Yiling , Zhao Qi , Ren Jian , He Weiling 
Published: September 2023

Abstract
BACKGROUND & AIMS:
Ulcerative colitis (UC) is linked to an increased risk of colitis-associated colorectal cancer (CAC), which accounts for approximately 15% of UC-related deaths. Despite this significant impact on patients, the mechanism behind how UC promotes cancer development remains unknown. The present study aims to investigate alterations in the immune microenvironment during the malignant transformation of UC, shedding light on the underlying mechanisms of UC carcinogenesis.
METHODS
We collected single-cell transcriptome samples of 41 healthy samples, 45 UC samples, and 148 colorectal cancer(CRC) samples from public databases. Using the UC-CRC signature, we were able to screen for CAC-like samples. Based on those datasets, several bioinformatics analyses were performed on 228,538 immune cells to evaluate the immune microenvironment from UC to CAC.
RESULTS
Using predefined UC-CRC signature, we screened 14 CAC-like samples and revealed an immune remodeling process from healthy tissue to UC and CAC-like samples, particularly involving the VEGFA_Macro cells and Treg cells. VEGFA_Macro cells was significantly enriched in UC and CAC-like samples, showed a phenotype alteration during disease progression, and expressed more inflammation-related genes and signal pathways. Additionally, the proportion of Treg cells gradually increased with disease progression, potentially promoting an immunosuppressive microenvironment. Comparative analysis of the immune microenvironment between CAC-like and sporadic CRC(sCRC) samples revealed higher levels of myeloid cells but reduced CD8 + T cells in CAC-like samples. Finally, we simplified the UC-CRC signature for ease of clinical use in screening CAC-like samples.
CONCLUSIONS
Our results may help improve the understanding dynamic change of immune microenvironment from UC to CAC and provide clues for further exploration of strategies to prevent carcinogenesis of UC.
Enhancing Corrosion Resistance of Anodic Oxide Films through Zirconium and Titanium Salts Composite Sealing Process: An Optimization and Morphological Study
Authors: Yang Kaixuan , Yang Fan , Huang Yijia , Yu Yang , Xu Wenyuan , Hu Lin , Liu Jianping 
Published: September 2023

Abstract
In this paper, zirconium and titanium are used as the main components of a sealing agent to seal composite holes. The effects of zirconium content, titanium content, pH, time, and temperature on the sealing effect of the oxide film in the environmentally friendly zirconium salt and titanium salt composite sealing process are studied by the orthogonal experiment and response surface method. The optimization results show that the best sealing conditions of the composite salt are: ammonium fluotitanate 6.0 g/L, ammonium fluozirconate 6.0 g/L, pH 4.0, and sealing time 20 min. The sealing temperature is 35°C. After analyzing the micro-morphology and surface elements of the oxide film sealed by composite salt, the authors found that the porous structure of the oxide film surface obviously disappeared after sealing, and the oxide film surface after sealing was mainly composed of Zr, Ti, O, Al, and other elements.
Highly expressed RPLP2 inhibts ferroptosis to promote hepatocellular carcinoma progression and predicts poor prognosis
Authors: Guo Jiaxing , Huang Meiyuan , Deng Shuang , Wang Haiyan , Wang Zuli , Yan Bokang 
Published: September 2023

Abstract
Background
RPLP2, an integral part of ribosomal stalk, plays an important role in the tumorigenesis of various cancers. However, its specific effect on HCC remains elusive.
Methods
TCGA, GTEx, HCCDB, HPA, UALCAN, MethSurv, TISIDB, K-M plotter, FerrDb, RNAactDrug, STRING, Cytoscape and R studio were conducted for bioinformatics analysis. RPLP2 expression level in HCC was verified by IHC and western blot. IHC was used to demonstrate the immune cell infiltration. Functional experiments including CCK8, transwell and colony formation assays, and nude mice xenograft model were performed for in vitro and in vivo validation. Western blot, IHC, CCK8 assay and detection of GSH and lipid ROS were adopted to determine the effect of RPLP2 on the ferroptosis of HCC cells.
Results
Here, we demonstrated that elevated level of RPLP2 is strongly associated with advanced clinicopathologic features, and predicts poor prognosis of HCC patients. Additionally, DNA methylation level of RPLP2 decreases in HCC, and significantly correlated with patients outcome. Moreover, high RPLP2 expression level is linked closely to the unfavorable immune infiltration. Most importantly, RPLP2 positively associates with ferroptosis suppressor GPX4, and inhibition of RPLP2 could lead to the acceleration of ferroptosis to suppress tumor progression of HCC. Last, drug sensitivity analysis predicts many drugs that potentially target RPLP2.
Conclusion
Together, our study reveals previous unrecognized role of RPLP2 in HCC, and provides new regulatory mechanism of ferroptosis, indicating RPLP2 may be a novel therapeutic target for HCC.
Object Recognition and Detection Using Convolutional Neural Network in Deep Learning
Authors: Shahalinejad Sahand , Nooshyar Mehdi , Fakheri Mir Majid , Imani Nima 
Published: September 2023

Abstract
Object recognition technology in the field of computer vision for finding and identifying objects in an image or video sequence. Humans recognize a multitude of objects in images with little effort, although the image of the objects may vary somewhat in different viewpoints, in many different sizes and scales or even when they are translated or rotated. Objects can even be recognized when they are partially obstructed from view. This task is still a challenge for computer vision systems. Many approaches to the task have been implemented over multiple decades In many computer vision systems, object detection is the first task being performed as it allows us to obtain further information regarding the detected object and about the scene. In recent years, the use of deep learning has attracted the attention of researchers, Deep learning uses multiple layers to extract raw features from high-level features the machine divides each complex concept into simpler concepts. The proposed method is based on AlexNet architecture. In this method, a convolutional neural network-based architecture with a small amount of data can detect objects in the image, the data used is the March database, so this process identifies thirty pictures in four separate classes with 100% accuracy.
Development of a health promoting sports clubs intervention: an intervention mapping process analysis
Authors: Hoye Aurélie Van , Johnson Stacey , Rostan Florence , Lemonnier Fabienne , Tezier Benjamin , Geidne Susanna , Donaldson Alex , Vuillemin Anne 
Published: September 2023

Abstract

The development of health promotion interventions using settings-based approaches in sports clubs remains a challenge. Intervention implementation in real world settings should be specific to a sports club’s context, especially considering the voluntary nature of the administration of most sporting organizations. The present paper aims to map and analyze the development of the PROSCeSS intervention, designed to support sports clubs to implement health promotion interventions. A case study design was used, mobilising the 5-steps of Intervention Mapping — needs assessment, matrix of change, theory of action, program design, plan for adoption and implementation and evaluation — to describe the multi-phase intervention and discuss its adaptation. Results encompass the main findings of a literature review and a concept mapping study as a needs assessment, a Delphi study and health promoting sports club intervention framework to design the matrix of change, a case study among sports clubs and sports federations to select the theory of action, the intervention co-construction to create the program, results from a pilot study to validate the plan for adoption and implementation and presentation of the e-PROSCeSS questionnaire creation and hybrid type-3 protocol as an evaluation method. The discussion explores the tensions created between evidence driven strategies and the needs and habits of sports club stakeholders, the use of top-down versus bottom-up approaches in multi-level design, and reflections on the recursive rather than linear process adopted by the authors. Future research should concentrate on intervention design description, as well as the importance of building iterative feedback when designing interventions.
Construction and validation of a neutrophil extracellular traps- related genes prognostic model in lung adenocarcinoma
Authors: Yang Wenxing , Deng Xinyue , Yin Haoyuan , Li Ying , Ji Yuxin , Lv Xiangyang , Ye Lianhua 
Published: September 2023

Abstract
Purpose The specific regulatory mechanism of neutrophil extracellular traps (NETs) in lung adenocarcinoma (LUAD) is unclear.
Methods LUAD related datasets were obtained via the cancer genome atlas (TCGA) and gene expression omnibus (GEO) databases. Differentially expressed genes (DEGs) between LUAD and control samples were sifted out, and the critical modules were yielded via weighted gene co-expression network analysis (WGCNA). NETs score of TCGA-LUAD set was estimated based on 136 NETs related genes. Further, DEGs1 between high/low scoring groups were interscted with the DEGs, critical module genes to acquire risk model genes via univariate Cox and least absolute shrinkage and selection operator (LASSO) analyses.
Results The six risk model genes were acquired, and the expression trends of which were all consistent in public datasets and clinical tissues. The survival percentages of LUAD patients increased with increasing risk score. Besides, the gene-drug network and the miRNA-mRNA-TF network targeting model genes were genereted, including ABCC2-Tamoxifen and hsa-mir-8485-IGF2BP1-ELK1.
Conclusion Through bioinformatic analysis, the study constructed a prognostic risk model according to IGF2BP1, ABCC2, BCL2L10, FIGF, EMR3, and WNT7A in LUAD, providing new ideas for related studies of LUAD.
Schisandrin B alleviates LPS induced mitochondrial damage in C28I2 cells
Authors: Hu Fei , Hu Wenjie , Xu Hongming 
Published: September 2023

Abstract
Osteoarthritis is a common joint disease characterized by damage to the joint cartilage that occurs throughout the entire joint tissue. This damage primarily manifests as pain in the affected area. In clinical practice, medication is commonly used to relieve pain, but the treatment’s effectiveness is poor and recurrent attacks are likely. Schisandrin B is the most abundant biphenylcyclohexene lignan found in the traditional Chinese medicine Schisandra chinensis, and it possesses various pharmacological effects. This study aims to investigate the protective effect of Schisandrin B on mitochondrial damage in osteoarthritis (C28I2 cells) under an inflammatory environment induced by LPS. Cell proliferation and activity, scratch tests, and LDH release tests are utilized to assess cell growth and migration ability. The immunofluorescence assay was used to detect the expression levels of proliferation and apoptosis proteins. The Western Blot assay was used to detect the expression levels of mitochondrial fusion and division proteins. The JC-1 assay was used to detect changes in mitochondrial membrane potential. The mitochondrial fluorescence probe assay was used to detect mitochondrial activity. Through research, it was found that Schisandrin B promotes the proliferation, growth, and migration of C28I2 cells, reduces apoptosis of C28I2 cells, balances mitochondrial fusion and division, stabilizes mitochondrial membrane potential, and promotes mitochondrial activity in an LPS-induced inflammatory environment.
Some like it (very) hot: the effects of climate change on nesting distribution of green turtles in the Mediterranean Sea
Authors: Mancino Chiara , Hochscheid Sandra , Maiorano Luigi 
Published: September 2023

Abstract
Climate change is reshaping global ecosystems at an unprecedented rate, with major impacts on biodiversity. Therefore, understanding how organisms can withstand change is key to identify priority conservation objectives. Marine ectotherms are being extremely impacted because their biology and phenology are directly related to temperature. Among these species, sea turtles are particularly problematic because they roam over both marine and terrestrial habitats throughout their life cycles. Focusing on green turtles (Chelonia mydas) in the Mediterranean Sea, we investigated the future potential changes of nesting grounds through time. We modeled the current distribution of nesting grounds including both terrestrial and marine variables, and we projected the potential nesting distribution across the Mediterranean basin under alternative future greenhouse gas emission scenario (2000–2100). Our models show an increase in nesting probability in the western Mediterranean Sea, irrespective of the climate scenario we consider. Contrary to what is found in most global change studies, the worse the climate change scenario, the more suitable areas for green turtles will potentially increase. The most important predictors were anthropogenic variables, which negatively affect nesting probability, and sea surface temperature, positively linked to nesting probability, up to a maximum of 24–25°C. The importance of the western Mediterranean beaches as potential nesting areas for sea turtles in the near future clearly call for a proactive conservation and management effort, focusing on monitoring actions (to document the potential range expansion) and threat detection.
Analyzing a full season of training load monitoring based on distance in Iranian Premier League Soccer players
Authors: Farhani Farid , Gharakhanlou Reza , Aghaalineghad Hamid , Amani-Shalamzari Sadegh 
Published: September 2023

Abstract
The present study was designed to investigate two aspects. Firstly, analysis of variations in acute load, chronic load, acute-chronic load ratio, monotony, and training strain during the preseason (PS), first and second half of the season (1st HS, 2nd HS). Secondly, comparing these indicators of training load in players' positions. Twenty-two elite soccer players from the Premier League of Iran (age: 27.2 4.5 years, professional experience: 6.2 4.3 years) were involved in this study. Players were monitored daily for 45 weeks through an 18 Hz global positioning system (GPS), to gather data on distance running (total running), distance running at 14 km/h (moderate intensity running), and distance running at a speed above 19.8 km/h (high-intensity running). One-way analysis of variance, followed by Tukey HSD post hoc test to analyze data. The pre-season has a significantly higher amount of acute load, chronic load, monotony and training strain, total running, and moderate-intensity running than the first half (p < 0.05) and the second half (p < 0.05). When comparing these training load indicators for high-intensity running, a significant difference was only observed between the PS and the 2nd HS (p 0.05, moderate ES). Also, no significant differences were observed between positions in total running and moderate intensity running. However, training load indices based on high-intensity running between positions showed external defenders vs center backs (p < 0.05), midfielders (p < 0.05) and strikers (p < 0.05) were significantly different. Furthermore, wingers had a significant difference in high-intensity running compared to central defenders (p < 0.05) and midfielders (p < 0.05). To conclude, this study demonstrated that acute load, chronic load, monotony, and training strain were more prevalent in the pre-season and slowly decreased during the season. External defenders and wingers experienced more acute load, chronic load, monotony, and training strain for high-intensity running during the season compared to other positions. Therefore, the results indicate that pre-season had a higher physical load than competition season, and players' positions experienced varying physical loads.
Mickelsson algebras via Hasse diagrams
Authors: Mudrov Andrey , Stukopin Vladimir 
Published: September 2023

Abstract

Let A be an associative algebra containing either classical or quantum universal enveloping algebra of a semi-simple complex Lie algebra g. We present a construction of the Mickelsson algebra Z(A, g) relative to the left ideal in A generated by positive root vectors. Our method employs a calculus on Hasse diagrams associated with classical or quantum g-modules. We give an explicit expression for a PBW basis in Z(A, g) in the case when A = U (a) of a finite-dimensional Lie algebra a ⊃ g. For A = U q (a) and g the commutant of a Levi subalgebra in a, we construct a PBW basis in terms of quantum Lax operators, upon extension of the ground ring of scalars to C[[ℏ]].
AMS classification codes: 17B10, 17B37.-
A detailed analysis of mixture stratification on flame displacement speed for syngas combustion
Authors: Patil Rahul , Sreedh Sheshadri 
Published: September 2023

Abstract
Gasoline direct injection (GDI) engines can provide higher thermal efficiency and lower emissions compared to conventional combustion techniques. The direct charge injection near the ignition source forms compositional stratification inside the combustion chamber. Compositional stratification inside the combustion chamber opens possibilities for ultra-lean and low-temperature combustion. In this paper, a 2D direct numerical simulation (DNS) has been performed to investigate the propagation of syngas flame in an equivalence ratio stratified medium. A spherically expanding flame has been initiated with a hotspot at the center of the domain. An open-source PENCIL code [Babkovskaia, 2011] is used to analyse the effect of stratification by simulating cases with varying integral scales of mixing (lϕ) and fluctuations of equivalence ratio (ϕ´). Effects of differential diffusion of species on flame propagation have also been examined by comparing results with cases with unity Lewis number (Le=1). The results show that with an increase in lϕ, flame propagation shows a non-monotonic behavior. With an increase in lϕ, the flame speed and extent of burning increase first and then decrease. With an increase in ϕ´, the flame speed and extent of burning decreased consistently. The peak reaction rate of fuel species is also observed to be shifted to a higher reaction progress variable (c) with increased stratification. The effect of stratification and differential diffusion has been analysed for four identified components of flame displacement speed (Sd) viz. reaction (Sr), normal diffusion (Sn), tangential (St), and inhomogeneity (Sz). Sr and Sn are observed to be major contributors to Sd. The magnitude of Sr shows reductions with an increase in stratification. In comparison, Sn does not show significant change with increased stratification. The variation of the contribution of chemical reactions to heat release rate with stratification is also analysed in this study. The results show that shifting of peak reaction rate of fuel species to higher c values results in variation in heat release rate contribution for chemical reactions.
Application of single mesh repair for bilateral inguinal hernia in Posterior sheath posterior approach single-incision laparoscopic totally extraperitoneal repair.
Authors: Zhang Yizhong , Ye Lebin , Chen junjie , Tang Jiansong , Wang Zhuoyin 
Published: September 2023

Abstract
This study aimed to assess the clinical effect of single mesh repair in the posterior sheath-posterior approach single-incision laparoscopic totally extraperitoneal repair (SIL-TEP) for bilateral inguinal hernia (BIH). We collected the relevant clinical data of 33 patients with BIH who underwent SIL-TEP via posterior sheath with a single mesh from May 2020 to July 2021, and analyzed the intraoperative and postoperative data. We evaluated the operation time, 24h postoperative visual analogue scale, intraoperative and postoperative complications of this group of patients. The results showed that 33 cases with BIH were successfully repaired in posterior sheath-posterior approach SIL-TEP without vascular or intestinal damage. All patients were followed up for 21 to 36 months, and no incision or mesh infection, recurrence or chronic pain occurred. We concluded that the transumbilical SIL-TEP repair of BIH with a single mesh through the posterior sheath-posterior is a safe and feasible procedure that has a cost-effectiveness advantage and deserves to be recommended.