bluuebunny/crossref_metadata_2025_split huggingface dataset:
Influenza A virus uses actomyosin for genome packaging at the cell membrane
Authors: Wang I-Hsuan , Usukura Jiro , Miyake Yasuyuki , Usukura Eiji , Narita Akihiro , Endou Toshiaki , Yamauchi Yohei , Kawaoka Yoshihiro 
Published: March 2023

Abstract
Influenza A virus encodes its genome in eight segments of viral ribonucleopropteins (vRNPs) replicated in the cell nucleus. Clustering of the eight vRNPs is thought to be a selective process promoted by negative-strand RNA interactions. Here, we visualized vRNP packaging at the plasma membrane using immuno-freeze-etching electron microscopy. After being transported to the inner surface of the plasma membrane, individual progeny vRNPs bound to actin filaments and clustered. Clustered vRNPs intermingled with actin filaments, myosin-II and the viral matrix protein M1, as shown by immunocytochemistry. Inhibition of myosin function by blebbistatin prevented proper vRNP clustering. Live-cell high-speed atomic force microscopy revealed blebbistatin reduced the budding kinetics of virions. Our findings highlight the importance of the actomyosin system in vRNP assembly and virion morphogenesis.
The effect of solution focused brief on overcome vaccine hesitancy: A randomized controlled study
Authors: Kaplan Mehmet , ERCİ Behice 
Published: March 2023

Abstract

This study was carried out to determine the effect of solution-focused approach on the elimination of vaccine hesitancy. The research was applied as a quasi-experimental model with pretest-posttest control group. The population of the study included 148 couples (female-male) who did not want to vaccinate their 0-24-month-old babies in Bingöl city, Turkey. Individuals were randomly selected using a simple random sampling method and probability sampling method. The numbers of people who completed the study in the experimental and control groups were 43 and 23, respectively. Four sessions of psychological counseling with a solution-focused brief were applied to each group. The attitude of the parents in the experimental group towards the vaccine changed positively. The confidence in the vaccination of babies between 0-24 months was determine to increase. Although there was no change in the behavior sub-dimension, official records were examined in 1 month after the completion of the study. Six families in the experimental group were determined to vaccinate their children. Solution-focused brief therapy was found to change positively parents’ attitudes and behaviors towards vaccination and to increase healtly lifestyle behaviors.
PpBZR1, a BES/BZR transcription factor, enhances cold stress tolerance by suppressing sucrose degradation in peach fruit
Authors: Zhang Shiyun , Cao Kefeng , Wei Yingying , Jiang Shu , Ye Jianfen , Xu Feng , Chen Yi , Shao Xingfeng 
Published: March 2023

Abstract

Brassinolides (BRs) are phytohormones that play numerous roles in a plants’ response to environmental stress. While BES/BZR transcription factors are essential components in BR signaling, their role in regulating postharvest fruit responses to cold stress is largely unknown. In this study, the application of 24-epibrassinolide (EBR) to peaches alleviated chilling injury (CI) during postharvest cold storage. We further characterized a key BES/BZR gene,PpBZR1, which regulates peach cold resistance. Transient expression PpBZR1in peaches showed that PpBZR1 inhibits PpVIN2 expression and VIN activity, resulting in an elevated level of sucrose, which protects fruit from CI. Arabidopsis expressing PpBZR1 had a high germination and seedling survival rate at low temperatures, which may be due to higher level of sucrose and lower oxidative damage. Mechanistically, we confirmed that PpBZR1 directly binds to the PpVIN2 promoter and functions as a negative regulator. In addition, PpCBF1/5/6 were induced by EBR treatment and AtCBFswere upregulated in transgenic Arabidopsis. Combined with previous findings, we hypothesize that PpBZR1 regulates PpVIN2 through a CBF-dependent manner. In conclusion, PpBZR1 expression is induced by EBR treatment during cold storage, and PpBZR1 futher inhibite sucrose degradation gene PpVIN2transcription via direct binding its promoter and indirectly regulating PpVIN2. This CBF-dependent and CBF-independent regulation results in slower sucrose degradation and higher chilling tolerance of peach.
Bone Disease Imaging through the Near-Infrared-II Window
Authors: Jin Dayong , Mi Chao , Zhang Xun , Yang Chengyu , Wu Jianqun , Chen Xinxin , Ma Chenguang , Wu Sitong , Yang Zhichao , Qiao Pengzhen , Liu Yang , Wu Weijie , Guo Zhiyong , Liao Jiayan , zhou jiajia , Guan Ming , Liang Chao , Liu Chao 
Published: March 2023

Abstract

Bone health related skeletal disorders are commonly diagnosed by X-ray imaging, but the radiation limits its use. Light excitation and optical imaging through the near-infrared-II window (NIR-II, 1000–1700 nm) can penetrate deep tissues without radiation risk, but the targeting of contrast agent is non-specific. Here, we report that lanthanide-doped nanocrystals can be passively transported by endothelial cells and macrophages from the blood vessels into bone marrow microenvironment. We found that this bone targeting scheme can be effective for longer than two months. We therefore developed an intravital 3D and high-resolution planar imaging instrumentation for bone disease diagnosis. We demonstrated the regular monitoring of 1 mm bone defects for 11 days in NIR-II window, with spatial resolution similar to X-ray imaging result, but more flexible use in prognosis. Moreover, the passive targeting can be used to reveal the early onset inflammation at the joints as the synovitis in the early stage of rheumatoid arthritis. Furthermore, the proposed method is comparable to micro computed tomography (µCT) in recognizing symptoms of osteoarthritis, including the mild hyperostosis in femur which is ~ 100 µm thicker than normal, and the growth of millimeter-scale osteophyte in the knee joint, which further proves the power and universality of our approach.
Seasonal analysis of aquatic water quality in a forestry area, MS, Brazil
Authors: Oliveira Víncler Fernandes Ribeiro de Oliveira , Pinto André Luiz Pinto , Lima Cesar Gustavo da Rocha Lima , Américo-Pinheiro Juliana Heloisa Pinê Américo-Pinheiro , Bacani Vitor Matheus Bacani 
Published: March 2023

Abstract

Water quality is one of the world's greatest concerns as it is a fundamental resource for life, and it must constantly be monitored. This study aimed to carry out seasonal monitoring of water quality in the hydrographic basin of the Urutu-MS stream, which is occupied by more than 50% of eucalyptus silviculture forests. Water samples were collected from five points seasonally between autumn 2019 and summer 2020. In total, twenty-three parameters were included, including temperature (Cº), pH, Dissolved Oxygen (DO, mg/L), Conductivity (EC ), Total Alkalinity (ALK), Organic Matter (OM), Chloride (Cl), Sulfate (SO42), Total Iron (Fe Total), Color, Turbidity (NTU), Total Dissolved Solids (TDS), Total Suspended Solids (TSS), Chemical Oxygen Demand (COD), Total Phosphorus (PT), Hardness, Aluminum (Al), Manganese (Mn), Magnesium (Mg), Sodium (Na), Calcium (Ca), Copper (Cu) and Dissolved Iron (Fe). The results showed that there is a difference in water quality between the seasons of the year depending on the different concentrations of physical-chemical parameters. Among the seasons, spring was classified in class II, while the others were classified in class I, showing the influence of the onset of rains after the dry winter. Interactions between parameters also followed conventional associations such as ALK and EC, TDS and EC, Color and Turbidity, TSS and Turbidity, EC and Ca, EC and Mg. The ALK and TDS were the main parameters that showed an influence of seasonality.
PRMT4 interacts with NCOA4 to inhibit ferritinophagy in cisplatin-induced acute kidney injury
Authors: Zhou Lizhi , Wang Yilong , Zhang Hao , Deng Zebin , Yan Shu , Kanwar Yashpal , Wang Yinhuai , Dai Yingbo , Deng Fei 
Published: March 2023

Abstract
Cisplatin-induced acute kidney injury (AKI) is commonly seen in clinical practice. Ferroptosis, an iron-catalyzed non-apoptotic cell death, is operative in the occurrence of cisplatin-induced AKI. Protein arginine methyltransferase (PRMT4), a member of type I PRMT family, was incorporated in various bioprocesses, but its role in renal injuries has not been investigated. In the present study, we aimed to explore the role of PRMT4 in cisplatin-induced AKI and its mechanism involved. Our data showed that PRMT4 was highly expressed in renal proximal tubular cells, and it was downregulated in cisplatin-induced AKI. Besides, genetic disruption of PRMT4 exacerbated, while its overexpression attenuated, cisplatin-induced redox injuries in renal proximal epithelia. Mechanistically, our work showed that PRMT4 interacted with NCOA4 to inhibit ferritinophagy, a process favoring lipid peroxidation to accelerate ferroptosis. Taken together, our study demonstrated that PRMT4 was bound to NCOA4 to attenuate ferroptosis in cisplatin-induced AKI, suggesting that PRMT4 might present as a new therapeutic target for cisplatin-related nephropathy.
Evaluation of expression of UHRF1 and its prognostic and clinicopathological value in human malignancies: A Systematic Review and Meta-analysis
Authors: Shu Qiang , Liu Xiaoling , Mou Tinggang , Xie Fei 
Published: March 2023

Abstract

Background:The mortality and recurrence of patients with cancer is of high prevalence. Ubiquitin-like with plant homeodomain (PHD) and RING finger domains 1 (UHRF1) is a promising nuclear protein gene. There is increasing evidence that UHRF1 is abnormally expressed in various tumors and is associated with cancer prognosis. This study was designed to identify the prognostic value of UHRF1 in human malignancies.Methods:we searched electronic databases up to February 18, 2023, including PubMed, Cochrane Library, Embase, MEDLINE, and Web of Science. Eligible studies that evaluated the clinicopathological and prognostic role of UHRF1 in patients with malignant tumors were included. The pooled odds ratios (ORs) and the hazard ratios (HRs) were calculated to assess the role of UHRF1 using Stata/SE 14.1 software.Results:10 studies included a total of 1461 patients on cancer patients were incorporated into the present meta-analysis. The combined results revealed that high expression of UHRF1 was significantly associated with unfavorable overall survival (OS) (HR=2.01, 95% CI: 1.23-3.29,P=0.005), worse disease free survival(DFS) (HR=1.86, 95% CI: 1.34-2.60,P=0.0002) in a variety of cancers. In additional, the increase in UHRF1 expression was also closely correlated with poor clinical stage (OR = 2.37, 95% CI: 1.59-3.51), lymph node metastasis (OR = 2.86, 95% CI: 1.97-4.16), and tumor recurrence (OR = 3.62, 95% CI: 2.30-5.28) in patients with cancer. Furthermore, the expression datasets of UHRF1 in human malignancies and paired normal tissues were downloaded from Gene Expression Profiling Interactive Analysis(GEPIA) showed that the expression level of UHRF1 was higher in most tumor tissues than in the corresponding normal tissues, which predicted a worse prognosis.The data analysis in Kaplan-meier Plotter also confirmed that patients with UHRF1 upregulation had a worse prognosis.Conclusions:UHRF1 may actas a valuable prognostic biomarker and a potential therapeutic target for patients with cancers.
Removal of thiophene compounds from model fuel with supported copper on activated carbon, adsorption kinetics, and isotherms
Authors: Golipour Haleh , Kazemi Bahador , Mafi Morteza , mokhtarani babak 
Published: March 2023

Abstract

In this study, the adsorption of thiophene compounds (TCs, comprised of thiophene (T), benzothiophene (BT), and dibenzothiophene (DBT)) from model fuel was performed with modified Active Carbon(AC). The single solute model fuel was prepared at 2000ppm, and the mixture concentration of components was performed for 3000ppm. Furthermore, thiophene adsorption from commercial fuel (kerosene) was studied. Based on the experimental results, the correlated parameters of adsorption isotherms, kinetic models, and Fisher factor were calculated. The pseudo-second-order model has the best fitting to experimental data, and the 10% Cu+supported on acid-washed Activated Carbon (A1CN10) has the best-adsorbed amount of TCs (T, BT, and DBT), being 78, 96, 100%, respectively. The physicochemical characterizations for adsorbents were verified by the N2adsorption-desorption surface area analyzer (BET), scanning electron microscopy (SEM), X-ray diffraction (XRD), and Energy Dispersive Spectroscopy (EDS). In addition, the adsorbent-regeneration process was performed by two agitating methods and ultrasound impact was studied.
Biotin-Thiamine Responsive Basal Ganglia Disease: A Retrospective Review of the Clinical, Radiological and Molecular Findings of Cases in Kuwait with Novel Variants
Authors: Aburezq Maryam , Alahmad Ahmad , Alsafi Rasha , Al-Tawari Asma , Ramadan Dina , Shafik Magdy , Abdelaty Omar , Makhseed Nawal , Elshafie Reem , Ayed Mariam , Hayat Abrar , Dashti Fatima , Marafi Dana , Albash Buthaina , Bastaki Laila , Alsharhan Hind 
Published: March 2023

Abstract

Background:Biotin-thiamine-responsive basal ganglia disease (BTRBGD) is a rare autosomal recessive neurometabolic disorder that is caused by biallelic pathogenicSLC19A3variants and characterized by subacute encephalopathy associated with confusion, convulsions, dysphagia, dysarthria, or other neurological manifestations.Methods:A retrospective analysis on the data registry in Kuwait Medical Genetics Center for cases diagnosed clinically, radiographically and confirmed genetically with BTRBGD.Results:Twenty cases from 12 different families were diagnosed with BTRBGD in Kuwait. Age of diagnosis ranges from newborn to 32 years, with a median age of 2 to 3 years. Most cases (83%) presented with confusion, dystonia, convulsions, or dysarthria, while three individuals were diagnosed pre-symptomatically during familial genetic screening. Symptoms resolved completely within 2-week of treatment in two-thirds of the symptomatic cases but progressed in five of them to severe cogwheel rigidity, dystonia and quadriparesis due to delayed presentation and management. Neuroradiological findings of the symptomatic cases revealed bilateral central necrosis of basal ganglia. Two novel homozygous missenseSLC19A3variants were detected in a Kuwaiti and a Jordanian individuals, in addition to the previously reported Saudi founder homozygous variant, c.1264A>G; p.(Thr422Ala) in the remaining cases. All cases are still alive receiving high doses of biotin and thiamine.Conclusion:This is the first study reporting the phenotype and genotype of 20 individuals with BTRBGD in Kuwait and describing two novelSLC19A3variants. It is a treatable neurometabolic disease that requires early recognition and treatment initiation; in addition to the importance of obtaining targeted molecular testing of the founder variant in patients with acute encephalopathy.
Comparison of the AmpFire® Multiplex HPV Assay to the Xpert® HPV Assay for detection of human papillomavirus and cervical disease in women with human immunodeficiency virus: A pragmatic performance evaluation
Authors: Moyo Sikhulile , Ramogola-Masire Doreen , Moraka Natasha , Tawe Leabaneng , Noubary Farzad , Motsumi Kesego , Manowe Godiraone , Zuze Boitumelo , Radibe Botshelo , Hungwe Faith TT , Mohammed Terence , Maphorisa Comfort , Shapiro Roger , Gaseitsiwe Simani , Luckett Rebecca 
Published: March 2023

Abstract
Background: Low- and middle-income countries (LMICs) account for nearly 85% of the global cervical cancer burden, yet have the least access to high-performance screening. International guidelines recommend human papillomavirus testing (HPV) as primary screening, yet implementation is inhibited by the cost of HPV testing.Atila AmpFire® HPV Assay (AmpFire) is both affordable and easy to use, and offers individual genotyping. The objective of this study was to compare the performance of the AmpFire HPV assay to the Xpert® HPV assay in detection of both HPV and clinically significant cervical disease.
Methods: We utilized stored cervical specimens from a prospective cohortstudy of women living with human immunodeficiency virus (HIV) in Botswana conducted from May to July 2018. Positive and negative percent agreement was calculated for the AmpFire and Xpert assays, as was detection of high-grade cervical dysplasia.
Results: 63 stored cervical specimens haddetectable DNA after thawing and were included in the analysis. The positive percent agreement was 91.2% (95%CI: 76.3-98.1) and negative percent agreement was 79.3% (95% CI: 60.3-92.0). Six cases positive by AmpFire but negative by Xpert were HPV genotypes 35, 52 (n=2), 58, 68, and co-infection with HPV 45 and 68. Both Xpert and AmpFire assays detected HPV in all 10 samples of women who had high-grade cervical dysplasia.
Conclusions: The AmpFire HPV assay demonstrated excellent analytic performance in both detection of HPV and clinically significant cervical disease. AmpFire HPV is a promising option to increase access to affordable, type-specific HPV screening for cervical cancer in LMICs.
Plant Disease Classification using Alex Net
Authors: Kewat Krishna 
Published: March 2023

Abstract
—In modern agriculture field, pest and disease identification is a major role of crop cultivation. Image classification by the use of deep convolutional neural networks of training and methodology used the facilitate a quick and easy system implementation. Pests and diseases are a threat to plant production, especially in India, but identification remains to be a challenge in massive scale and automatically. Collecting images from Image Net dataset. The results show that we can effectively detect and recognize the rice diseases and pests including healthy plant class using a deep convolutional neural network, with the best accuracy of 96.50%. The significantly high success rate makes the model a really useful advisory or early warning tool, and an approach that would be further expanded to support an integrated plant disease identification system to work in real cultivation conditions.
Association of AST and ALT Ratio with Incidence of Diabetes in Chinese Adults: A Retrospective Cohort Study
Authors: Yao Qing , Zhang Wei , Wu Weixun , Wang Xinyue , Jia Sheng , Huang Jiale , Gao Jian 
Published: March 2023

Abstract

Purpose

Little research exits on the effect of the aspartate aminotransferase/alanine aminotransferase (AST/ALT) ratio on the incidence of diabetes mellitus (DM). This study used the large-scale cohort study data of the Chinese population to explore the effect of the AST/ALT ratio on the incidence of DM.

Patients and methods

The data was downloaded from a computerized database (DATADRYAD, available at www.datadryad.org) including 32 sites and 11 cities in China which were collected between 2010 and 2016 by the Rich Healthcare Group in China. This study was a retrospective cohort study that, included 86,145 participants for the secondary analysis. Multivariable Cox proportional hazards regression and a two-piecewise linear regression model were used to explore the relationship between the AST/ ALT ratio and DM.

Results

The AST/ALT ratio was negatively correlated with the incidence of DM after adjusting for potential co-variables [HR = 0.44, (95%CI:0.38–0.51), P < 0.001]. By using the two-piecewise linear regression model, the inflection point was 1.3. On the left of the inflection point, the effect size was HR = 0.3 (0.2–0.3, P < 0.001). On the right side of the inflection point, the effect size was HR = 0.9 (0.7 to 1.2; P = 0.561).

Conclusion

The AST/ALT ratio could be an independent predictor of incident DM and was negatively correlated with DM events.
An unsupervised lightweight network for multispectral palmprint recognition
Authors: Imene Seghier , Chaa Mourad , Aiadi Oussama , Fouad Chebbara , Khaldi Belal 
Published: March 2023

Abstract
In this article, we propose an unsupervised convolutional deep learning network with a single layer for multispectral palmprint recognition. We refer to this method as GradNet because it depends on the magnitude and direction of the response from data-driven filters. GradNet generates a filter bank from training images using DCT. Then, there a twofold layer, which comprises two operations namely convolution using learned filters and computation of computation of gradient images (Magnitude and Direction). The binary hashing process can effectively and efficiently eliminate the over-fitting by combining different filter responses into a single feature map. The histograms of gradient magnitude, direction images has been constructed according to the feature map. The histograms of gradient magnitude, direction and single feature map are then normalized, using power-L2 rule, to cope with illumination disparity and combined. The holistic feature extraction method has been employed to attain salient characteristics. Finally, for the features matching the cosine Mahalanobis distance has been used for multispectral palmprint recognition. The proposed system has been evaluated on publicly available multispectral palmprint database of the Hong Kong Polytechnic University. Experimental analyses show that the proposed method demonstrate that our method is capable of competing with many existing state-of-the-art multispectral palmprint recognition techniques as well as outperforming many others.
Prediction of post-operative clinical parameters in posterior scoliosis surgery using an adaptive neuro-fuzzy interface system
Authors: ABEDI Rasoul 
Published: March 2023

Abstract
Background and Objective: Postoperative clinical indices should be estimated accurately in scoliosis correction surgeries, which have been analyzed in various studies such as experimental (in vitro or in vivo) trials through different modeling methods (finite element or multibody analysis). These costly and time-consuming methods can only be conducted on a large number of scoliotic patients. An adaptive neuro-fuzzy interface system (ANFIS) is used in this study to estimate the postoperative cobb and thoracic kyphosis angles in adolescent idiopathic scoliosis patients undergoing posterior scoliosis correction surgeries.
Methods: Four groups of 55 patients with distinct preoperative clinical indices (thoracic cobb and pelvic incidence) were considered the ANFIS inputs, whereas postoperative thoracic cobb and kyphosis angles were used as the outputs. For robustness evaluation, the predicted values of postoperative angles were compared with measurements by calculating the root mean square errors and clinical correction deviation indices (the relative deviation of postoperative predicted angles from the real angles).
Results: The least root mean square errors (3.0º and 6.3° for the main thoracic cobb and thoracic kyphosis estimations, respectively) were recorded in the group with the main thoracic cobb, pelvic incidence, thoracic kyphosis, and T1 spinopelvic inclination used as inputs. The clinical correction deviation indices were calculated 0.0086 and 0.0641 for cobb angles in two cases and 0.0534 and 0.2879 for thoracic kyphosis in two other cases.
Conclusion: Greater differences between preoperative and postoperative cobb angles compared with those of thoracic kyphosis decreased the root-mean-square errors and clinical deviation indices but improved accuracy.