bluuebunny/crossref_metadata_2025_split huggingface dataset:
Elucidation of the Molecular Basis and Cellular Functions of Vinculin-Actin Directional Catch Bonding
Authors: Campbell Sharon , Chirasani Venkat , Khan Mohammad , Malavade Juilee , Dokholyan Nikolay , Hoffman Brenton 
Published: January 2023

Abstract
The ability of cells and tissues to differentially resist or adapt to mechanical forces applied in distinct directions is mediated by the ability of load-bearing proteins to preferentially maintain physical linkages in certain directions. However, the molecular basis and biological consequences of directional force-sensitive binding are unclear. Vinculin (Vcn) is a load-bearing linker protein that exhibits directional catch bonding due to interactions between the Vcn tail domain (Vt) and filamentous (F)-actin. We developed a computational approach to predict Vcn residues involved in directional catch bonding and produced a set of associated Vcn variants with unaltered Vt structure, actin binding, or phospholipid interactions. Incorporation of these variants into Vcn biosensors did not perturb Vcn conformation, but reduced Vcn loading consistent with loss of directional catch bonding. Expression of Vcn variants perturbed the coalignment of FAs and F-actin and directed cell migration, establishing key cellular functions for Vcn directional catch bonding.
Needlescopic suturless repair of congenital inguinal hernia: A randomized controlled study
Authors: Shalaby Rafik Yousef , Ali Mohamed Abdelmaboud , Daboos Mohammad Alsayed , Ahmed Yousef Mohamed , Helal Ahmed Abdelghafar , Gamman Ibrahim Ahmed 
Published: January 2023

Abstract
Background: Many techniques have been published in the literature about laparoscopic treatment of congenital inguinal hernia [CIH]. Division of the sac, with suturing the peritoneal defect has been advocated by many authors. Others stated that peritoneal disconnection alone is enough. This study aimed to compare the results of needlescopic disconnection of CIH sac with or without peritoneal defect suturing as regards feasibility, operative details, recurrence rate, and postoperative complications.
Patients and Methods: This study has been conducted prospectively on children operated upon by needlescopic separation of hernia sac at its neck with or without peritoneal defect suturing over the Internal Inguinal Ring [IIR] in the period from January 2020 to December 2022.
Results: A total of 230 patients with 260 hernial defects were corrected by needlescopic disconnection with either suturing or without suturing of the defect. They comprised of 141 (61.3%) males, and 89 (38.7%) females. Their ages ranged from 0.5 to 12 years (mean=5.14 ± 2.79). Patients were distributed into group A (N=116 patients) and group B (N=114 patients). In sac separation and closure of the defect group A, the mean operative time was 27.98 ± 2.89 [Rang = from 20 to 30 minutes] for unilateral cases and 37.29 ± 4.68 [Range = from 25 to 40 minutes] for bilateral one, whereas in group B, the mean operative time was 20.37 ± 2.37 [Range=from 15 to 25 minutes] for one-sided and 23.38 ± 2.22 [Range =from 20 to 25 minutes] in two-sided cases.
Conclusion: Needlescopic separation of hernia sac with suturing of peritoneal defect and disconnection without suturing for pediatric inguinal hernia repair, are feasible, safe, and less invasive with no recurrence and outstanding cosmetic results. However, non- suturing of peritoneal defect gives significantly shorter operative time than suturing of peritoneal defect
Evaluation and mapping of precipitable water in Southeast Asia using ground-based and satellite data for solar energy application
Authors: Sabooding Rusmadee , Taweekun Juntakan , Fawzi Mas 
Published: January 2023

Abstract
This article presents column integrated precipitable water (PW) readings from a NASA satellite and fourteen sites of the Aerosol Robotic Network (AERONET). The Terra satellite was compared with that obtained from ground-based observations in the tropics. It was found that the PW from the MODIS aboard the Terra satellite and those obtained from ground-based observations were in good agreement, with a root mean square error (RMSE) of 4.27% and a mean bias error (MBE) of 0.03% for the monthly. Develop empirical model to estimate the PW using data from relative humidity (AIRS) and temperature (AIRS) of ambient air. The model was developed to determine the daily and monthly PW based on PW data obtained from these stations over a 5-year period (2012–2016) and it was then validated against independent data for the previous 4-year period (2017–2020). It can be seen that the monthly PW values calculated using the empirical model had a RMSE of 3.64%. Develop a model2 to allow users to estimate the PW, namely relative humidity (AIRS) and temperature (GLDAS Model) of ambient air. This model2 performs reasonably when tested against an independent data set with the root mean square error (RMSE) and mean bias error (MBE) of 9.30% and − 0.05%, respectively (daily) and 3.58% and 0.02%, respectively (monthly). This research presents PW resource maps generated with the Geographic Information System (GIS) by satellite-based data from the Terra satellite for the 21-year period (2000–2020) and from AIRS (relative humidity and temperature) for the nineteen-year period (2002–2020). According to the monthly PW maps, Southeast Asia's PW values are generally high during the wet season (May to October) and low during the dry season (November to April) and according to the annual PW map, areas near the sea have high PW.
Poor tibiofemoral congruity increases the risk of posterior cruciate ligament injury.
Authors: Wang De-ning , Zhang Ke-fan , Shi Hui , Du Chang-ling , Wang Xin 
Published: January 2023

Abstract
Background Studies suggest that posterior cruciate ligament (PCL) injury may be related to the anatomical morphology of the distal femur and proximal tibia.
Purpose In retrospectively, we studied whether there were differences in the morphology and correlation between the distal femur and proximal tibia between the PCL injury group and the age and sex matched PCL intact control group, and then found the risk factors for posterior cruciate ligament rupture.
Methods From January 2015 to August 2022, 98 male patients with knee injury or discomfort due to non-contact mechanism were selected in the posterior cruciate ligament rupture group (n=49) and the posterior cruciate ligament intact group (n=49) by MRI images and arthroscopy. The posterior tibial slope (PTS), intercondylar notch width (INW), bicondyle width (BCW), angle of intercondylar notch (AIN), tibial spine width (TSW), and tibial platform width (TPW) were measured on X-ray and MRI coronal images, and the notch width index (NWI) was obtained by calculating the ratio of the intercondylar notch width to the bicondyle width, and the ratio of the tibial spine width to the tibial platform width was calculated to obtain the tibial eminence width index (TEWI). The ratio of the tibial spine width to the intercondylar notch width was calculated to obtain the tibiofemoral consistency index (TFCI) to describe the matching between the tibial spine and intercondylar notch.
Results Univariate analysis showed that the posterior tibial slope, tibial spine width, tibial eminence width index, and tibiofemoral consistency index were lower than those in the posterior cruciate ligament rupture group (P < 0.05), and there was no significant difference in age, intercondylar notch width, bicondyle width, notch width index, angle of intercondylar notch and tibial platform width between the two groups (P > 0.05). Multivariate logistic regression analysis showed that the tibiofemoral consistency index was an independent risk factor for posterior cruciate ligament rupture (P < 0.05). The receiver operator characteristic curve (ROC curve) shows that the tibiofemoral consistency index has some predictive value for posterior cruciate ligament rupture, the area under the curve was 0.785, 95% CI (0.695, 0.875).
Conclusions The tibiofemoral consistency index is an independent risk factor for posterior cruciate ligament rupture, and has a certain predictive value for posterior cruciate ligament rupture, and the lower the ratio, the worse the match between the tibial spine and the intercondylar notch, and the higher the risk of posterior cruciate ligament rupture.
DFT study on molecular structure, spectroscopic properties, Hirshfeld surface and molecular docking reveals the potential of flavones based on experimental and theoretical investigations
Authors: Manaka A. , Uvarani R. 
Published: January 2023

Abstract
In the present investigation, theoretical study on molecular geometry, vibrational, pharmaceutical and electronic properties of 3,4,5,7-tetrahydroxyflavone (7THDFN) and 3,4,7,8-tetrahydroxyflavone (8THDFN) were carried out using various functional levels at 6-311 + + G(d,p) as basis set. The results of the optimized geometrical parameters are presented and compared with the experimental values. The harmonic vibrational frequencies were calculated and the computed FT-IR and FT-Raman spectra have been compared with the experimental data. The 1H and 13C NMR chemical shifts were calculated using Gauge Independent Atomic Orbital (GIAO) approach. As an outcome, we found a good agreement between the experimental and computational structural values for FT-IR, FT-Raman, and NMR spectra. The molecular frontier orbital analysis was used to determine the charge transfer interaction, chemical reactivity, and non-linear optical properties of the designed molecules at the PBEPBE level theory. In addition, the molecular electrostatic potential surface maps were plotted to determine the chemical reactivity and the intermolecular interactions of the bioactive molecules. All these theoretical and experimental calculations will help us to characterize the chemical or biological properties of the designed drug molecules. The molecular modeling programs were also performed to estimate the pharmaceutical activities of the flavone compounds against human breast cancer. Finally, the in vitro cytotoxicity of bioactive molecules against human MCF-7 cell lines measured in MTT assays.
Health Services Use After Covid-19 Infection in Persons With Non-communicable Diseases
Authors: Vieira Yohana Pereira , Caputo Eduardo Lucia , Rocha Juliana Quadros Santos , Duro Suele Manjourany Silva , Saes Mirelle de Oliveira 
Published: January 2023

Abstract
The COVID-19 pandemic has triggered several changes in health care service at all levels, the primary and specialized care services had to be restructured hampering longitudinal follow-up. Thus, this scenario led to impaired disease management of patients with non-communicable diseases (NCDs), worsening the clinical picture of their pre-existing disease. This study aimed to evaluate the use of health services after COVID-19 infection in persons with non-communicable diseases (NCDs). This is a cross-sectional study in the city of Rio Grande with individuals aged 18 or older, from the urban area of the city, who had a positive diagnosis of COVID-19 by reverse transcription followed by polymerase chain reaction (RT-PCR) from December 2020 to March 2021. Participants were asked if they attend any healthcare services after COVID-19 infection. Regarding exposure, participants were asked if they had a medical diagnosis of the following NCDs: systemic arterial hypertension (SAH), diabetes mellitus (DM), respiratory diseases (RD) and heart diseases (HD). Descriptive analyzes were carried out and the Poisson regression was used to verify associations, with robust adjustment of variance. The likelihood of using healthcare services among participants with NCDs was up to four times when compared to individuals without it. Specifically, participants with NCDs were 67% (95% CI 1.33; 2.11) more likely to use urgent and emergency services. When analyzed by specific NCDs, only HD was associated with emergency room and emergency care unit use (PR 2.14; CI95%1.30-3.54; PR1.70; CI95%1.31-2.20, respectively). An increased use of specialized services (i.e., cardiologist) is highlighted among individuals with at least one NCD (PR:3.77; CI95%2.97-4.80), SAH (PR:3.47; CI95%2.97-4.31) and DM (PR:3.87; CI95%3.11-4.81). People with NCD required more healthcare services after COVID-19 infection, regardless of their disease when compared to those without it.
Effectiveness of exercise rehabilitation from the hospital to the community in individuals with shoulder disorders: a study protocol
Authors: Jeong Minah , Moon Chang Won , Kim Young Wook , Jung Il-Young 
Published: January 2023

Abstract
Background

Rotator cuff injury (RCI) and adhesive capsulitis of the shoulder (ACS) are musculoskeletal disorders (MSDs) characterized by limited motion of the shoulder. As symptoms of MSDs worsen over time, constant management and exercise are necessary. We hypothesized that shoulder pain can be managed through full-cycle rehabilitation exercises. Therefore, the purpose of this study is to confirm the effect of full-cycle rehabilitation exercises by providing exercise education in the community after hospital exercise intervention in subjects with RCI and ACS.

Methods

This is the protocol for a prospective, randomized controlled trial. Sixty subjects with shoulder pain will be recruited from the rehabilitation clinics of an urban medical hospital. Then, subjects will be randomly allocated into two groups. The intervention group will underwent 12 weeks of rehabilitation exercises in both the hospital and community, while the control group will conduct home exercises for 4 weeks using videos provided by the researchers. The main outcomes will be the change in shoulder pain and score of activities of daily living from initial evaluation to 3 months. The secondary outcomes will be functional measurements, such as ROM and the Manual Muscle Test (MMT), Timed Up and Go test (TUG), 10-meter walk test (10MWT), and Berg Balance Scale (BBS) scores, and will include subject-reported outcomes at multi-time points up to 3 months after the intervention.

Discussion

This is a randomized controlled trial to study the effectiveness of full-cycle rehabilitation exercises for shoulder pain. The results of this study will determine whether exercise from the hospital to the community is effective in subjects with RCI or ACS and will present evidence for the proper community connection guidelines for sustainable rehabilitation.
Trial registration:
Assessment of the dynamics of bike-sharing for students’ mobility in Kigali City
Authors: Ntamwiza Jean Marie Vianney , Bwire Hannibal 
Published: January 2023

Abstract
Compared to other modes of transportation available today, bike sharing is favored in more than 800 cities for its low environmental impact. Members of the bike-sharing program can use bikes from any related bike-sharing network. Users get the advantages of the system without bearing the burdens of ownership. There have been four versions of bike-sharing used thus far. The application of smart cards is a relatively new innovation introduced in bike-sharing systems. The new innovation allowed for the beginning of data availability through Stations and it has facilitated data accessibility for researchers. But papers tracking the evolution of bike-sharing membership and activities are rare. In contrast, studies focusing on students' mobility are few and far between. Within the framework of bike sharing, the dynamics of bike sharing are discussed in the literature of this paper. This has been accomplished by keeping tabs on how many people used the system over time, since this provided important information about the system's functioning and might be used as a basis for allocating stations. Data from GuraRide, Kigali - Rwanda used in this study. And 10,073 bike-share trips taken between December 9, 2021 and May 30, 2022 were analyzed. Then, a GIS software was used to map the locations of stations and corridors, and Python software was used for statistical analysis. A random forest algorithm was used to assess changes in stations and corridors usage. The findings indicated positive variations among system users and stations usage.
Clinical spectrum of ear, nose and throat foreign bodies at a tertiary hospital in Zanzibar, Tanzania
Authors: Abraham Zephania Saitabau , Kahinga Aveline Aloyce , Khamis Khamis Omar , Liyombo Edwin 
Published: January 2023

Abstract
Objective: Presence of foreign bodies (FBs) in the ears and upper aerodigestive tract is a common encounter in children unlike adults in otorhinolaryngology practice. Such foreign bodies form a major part of emergencies that the otorhinolaryngologist needs to attend. The high prevalence in children can be attributable to the inquisitive nature of children and their tendency to explore the environment. This study was a cross sectional study that aimed to determine the clinical spectrum of such foreign bodies at the largest tertiary hospital in Zanzibar, Tanzania.
Results: In this study, there were more females, 56(58.9%) than males, 39(41.1%) with female to male ratio being 1.4:1. Children aged less than 10 years predominated in this study, 69(72.6%). The nose, 36(37.9%) and ear, 29(30.5%) were the commonest sites were foreign bodies lodged followed by the pharynx, 22(23.2%) and esophagus, 10(8.4%). Regarding types of foreign bodies, inorganic 49(51.6%) types predominated and were mostly coins 17(17.9%). Majority of foreign bodies were removed in less than 24 hours (53.6%) and complications were found in 29(30.5%) patients and being more with nasal foreign bodies. Majority of those with complications presented 24 to 72 hours post lodging of foreign bodies
Association between Controlling Nutritional Status Score and Severity of Coronary artery stenosis in elderly patients
Authors: Zhong Guofu , Chen Mingtai , Chen Junteng , Xu Mujuan , Han Yue , Chang Xiao , Men Ling , Wang Ling 
Published: January 2023

Abstract
Background
The Controlling Nutritional Status (CONUT) Score is a tool to evaluate the body nutritional state. We investigated whether the CONUT score was associated with the incidence of coronary artery disease (CAD) and the severity of coronary artery stenosis in elderly patients.
Methods
This study involved 3296 elderly inpatients undergone coronary angiography. A demographic and clinical profile, as well as a Gensini score, were collected. The CONUT score were evaluated by the score system. There was a correlation analysis, a multivariable logistic regression analysis, and an analysis of the receiver operating characteristic curve (ROC curve).
Results
Based on the quartiles of CONUT scores, 3296 elderly inpatients were divided into four groups. There were significant differences in the vessel of coronary artery stenosis among the four groups (P < 0.05). The CONUT score was significantly positively correlated with Gensini score (r = 0.416, p < 0.001). After we divided enrolled patients into four groups by Gensini score and degree of coronary artery stenosis, the CONUT score was closely associated with the severity of coronary artery stenosis. For multivariable logistic regression, the CONUT score was risk factors of incidence of CAD and process of atherosclerosis progression after adjustment for potential confounding factors. The AUCs were 0.807 (95% CI: 0.790–0.824) for CAD, 0.717 (95% CI: 0.692–0.742) for mild stenosis, 0.842 (95% CI: 0.824–0.861) for moderate stenosis and 0.887 (95% CI: 0.867–0.908) for Severe stenosis by receiver operating characteristic analysis.
Conclusions
The CONUT score confers a predict factor of CAD in elderly patients, which also indicated the prognosis value in atherosclerosis progression.
Measuring perceived, wished and actual knowledge of healthcare providers about pediatric palliative care: development and validation of an online questionnaire in Italy
Authors: Zanello Elisa , Vecchi Roberta , Zamagni Giulia , Biagi Maria Celeste , Bruno Irene , Cragnolin Elisa , Danielli Elisabetta , Paoletti Silvia , Rabusin Marco , Ronfani Luca , Valente Emanuelle Pessa 
Published: January 2023

Abstract
Background. There is a lack of validated tools to evaluate the healthcare professionals’ competences on Pediatric Palliative Care (PPC) and Pain Therapy (PT). The aim of this study is to document the development and validation of a questionnaire to assess perceived, wished and actual knowledge of healthcare workers on PPC/PT.
Methods. On the basis of the Italian Society for Palliative Care PPC Core Curriculum (CC) for physicians, nurses and psychologists, an online questionnaire was developed in four sequential steps. Construct validity, coherence of the instrument, and educational needs of participants were assessed after a field testing in a referral hospital, Northeast Italy.
Results. One-hundred-five respondents completed the online questionnaire, from January 1st to July 31th 2019. Findings showed high internal consistency for both scales of perceived and wished knowledge (⍺=0.95 and ⍺=0.94, respectively). Psychologists reported higher levels of self-perceived skills on psychosocial needs of child and family at the end of life (p=0.006), mourning (p=0.003) and ethics and deontology in PT/PC (p=0.049). No differences were found by profession in terms of wished knowledge. Regarding actual knowledge assessment psychologists provided the bigger number of correct answers (p=0.022).
Conclusions. The questionnaire showed promising psychometric properties as a multidisciplinary instrument to measure health professionals’ knowledge on PPC/PT. Our findings suggest a need of continuous training in this topic and present possible content to be addressed by decision makers when organizing future training programs and/or educational events.