bluuebunny/crossref_metadata_2025_split huggingface dataset:
Wealth inequalities in physical and cognitive impairments across Japan and Europe: the role of health expenditure and infrastructure
Authors: Le Dung D. , Quashie Nekehia T. , Brandt Martina , Ibuka Yoko 
Published: June 2023

AbstractAlthough prior research has provided insights into the association between country-level factors and health inequalities, key research gaps remain. First, most previous studies examine subjective rather than objective health measures. Second, the wealth dimension in health inequalities is understudied. Third, a handful of studies explicitly focus on older adults. To bridge these research gaps, this study measures wealth-related inequalities in physical and cognitive impairments and examines the extent to which welfare states moderate wealth inequalities in physical and cognitive impairments among older people across Japan and Europe. We utilized harmonized data on non-institutionalized individuals aged 50–75 from the Japanese Study of Aging and Retirement (JSTAR) and the Survey of Health, Ageing and Retirement in Europe (SHARE) (N = 31,969 for physical impairments and 31,348 for cognitive impairments). Our multilevel linear regression analyses examined whether national public health spending and healthcare access resources explained cross-country differences in wealth inequalities in physical and cognitive impairments. We applied a concentration index to quantify the degree of wealth inequalities in impairments. The findings indicate that inequalities in both impairment outcomes favored wealthier individuals in all countries, but the magnitude of inequality varied by country. Furthermore, a higher share of public health spending, lower out-of-pocket expenditure, and higher investment in healthcare resources were associated with lower wealth inequalities, especially for physical impairments. Our findings suggest that different health interventions and policies may be needed to mitigate specific impairment inequalities.
Discovery of extracellular vesicle-delivered miR-185-5p in the plasma of patients as an indicator for advanced adenoma and colorectal cancer
Authors: Shi Yun-Jie , Fang Yu-Xiang , Tian Tong-Guan , Chen Wei-Ping , Sun Qiang , Guo Fang-Qi , Gong Pi-Qing , Li Chun-Mei , Wang Hao , Hu Zhi-Qian , Li Xin-Xing 
Published: June 2023

Abstract
Background
We aimed to evaluate whether extracellular vesicles (EV)-derived microRNAs (miRNAs) can be used as biomarkers for advanced adenoma (AA) and colorectal cancer (CRC).

Methods
We detected the changes in the plasma EV-delivered miRNA profiles in healthy donor (HD), AA patient, and I-II stage CRC patient groups using miRNA deep sequencing assay. We performed the TaqMan miRNA assay using 173 plasma samples (two independent cohorts) from HDs, AA patients, and CRC patients to identify the candidate miRNA(s). The accuracy of candidate miRNA(s) in diagnosing AA and CRC was determined using the area under the receiver-operating characteristic curve (AUC) values. Logistic regression analysis was performed to evaluate the association of candidate miRNA(s) as an independent factor for the diagnosis of AA and CRC. The role of candidate miRNA(s) in the malignant progression of CRC was explored using functional assays.

Results
We screened and identified four prospective EV-delivered miRNAs, including miR-185-5p, which were significantly upregulated or downregulated in AA vs. HD and CRC vs. AA groups. In two independent cohorts, miR-185-5p was the best potential biomarker with the AUCs of 0.737 (Cohort I) and 0.720 (Cohort II) for AA vs. HD diagnosis, 0.887 (Cohort I) and 0.803 (Cohort II) for CRC vs. HD diagnosis, and 0.700 (Cohort I) and 0.631 (Cohort II) for CRC vs. AA diagnosis. Finally, we demonstrated that the upregulated expression of miR-185-5p promoted the malignant progression of CRC.

Conclusion
EV-delivered miR-185-5p in the plasma of patients is a promising diagnostic biomarker for colorectal AA and CRC.
Trial registration The study protocol was approved by the Ethics Committee of Changzheng Hospital, Naval Medical University, China (Ethics No. 2022SL005, Registration No. of China Clinical Trial Registration Center: ChiCTR220061592).
Influence of depression and interpersonal support on adherence to antiretroviral therapy among people living with HIV
Authors: Nutor Jerry John , Gyamerah Akua O. , Alhassan Robert Kaba , Duah Henry Ofori , Thompson Rachel G.A. , Wilson Natalie , Harris Orlando , Gutierrez Jose , Hoffmann Thomas J. , Getahun Monica , Santos Glenn-Milo 
Published: June 2023

Abstract
Background
Poor adherence and under-utilization of antiretroviral therapy (ART) services have been major setbacks to achieving 95-95-95 policy goals in Sub-Saharan Africa. Social support and mental health challenges may serve as barriers to accessing and adhering to ART but are under-studied in low-income countries. The purpose of this study was to examine the association of interpersonal support and depression scores with adherence to ART among persons living with HIV (PLWH) in the Volta region of Ghana.

Methods
We conducted a cross-sectional survey among 181 PLWH 18 years or older who receive care at an ART clinic between November 2021 and March 2022. The questionnaire included a 6-item simplified ART adherence scale, the 20-item Center for Epidemiologic Studies Depression Scale (CES-D), and the 12-item Interpersonal Support Evaluation List-12 (ISEL-12). We first used a chi-squared or Fisher’s exact test to assess the association between these and additional demographic variables with ART adherence status. We then built a stepwise multivariable logistic regression model to explain ART adherence.

Results
ART adherence was 34%. The threshold for depression was met by 23% of participants, but it was not significantly associated with adherence in multivariate analysis(p = 0.25). High social support was reported by 48.1%, and associated with adherence (p = 0.033, aOR = 3.45, 95% CI = 1.09–5.88). Other factors associated with adherence included in the multivariable model included not disclosing HIV status (p = 0.044, aOR = 2.17, 95% CI = 1.03–4.54) and not living in an urban area (p = 0.00037, aOR = 0.24, 95% CI = 0.11–0.52).

Conclusion
Interpersonal support, rural residence, and not disclosing HIV status were independent predictors of adherence to ART in the study area.
Physical discipline as a normative childhood experience in Singapore
Authors: Sudo Mioko , Won Ying Qing , Chau Winnie W. Y. , Meaney Michael J. , Kee Michelle Z. L , Chen Helen , Eriksson Johan Gunnar , Yap Fabian , Rifkin-Graboi Anne , Tiemeier Henning , Setoh Peipei 
Published: June 2023

AbstractBackgroundThe cultural normativeness theory posits that specific parenting behaviors can be interpreted as displays of appropriate parenting in contexts where they are deemed normative. Previous studies suggest high acceptance of physical discipline in Singapore, where strict parenting could be interpreted as care for the child. However, there is a lack of studies on the local prevalence and implications of physical discipline. This study aimed to investigate the prevalence of Singaporean children experiencing parental physical discipline, longitudinal changes in this prevalence, and how exposure to physical discipline relates to children’s evaluation of their parents’ parenting.MethodsParticipants were 710 children with parental reports of physical discipline at one or more assessments at ages 4.5, 6, 9, and 11 years in the Growing Up in Singapore Towards healthy Outcomes birth cohort study. Parental reports of physical discipline were obtained using the Parenting Styles and Dimensions Questionnaire or the Alabama Parenting Questionnaire across the four assessments. Child reports of their parents’ care and control were obtained using the Parental Bonding Instrument for Children at the age 9 assessment. Prevalence was specified as being exposed to at least one physical discipline at any frequency. A generalized linear mixed model was performed to examine whether children’s age predicted their exposure to physical discipline. Linear regression analyses were conducted to investigate whether children’s exposure to physical discipline predicted their evaluation of their parents’ parenting.ResultsThe prevalence of children experiencing at least one physical discipline was above 80% at all ages. There was a decrease in this prevalence from age 4.5 to 11 years (B = − 0.14,SE = 0.01, OR = 0.87,p < 0.001). The more frequent the paternal physical discipline children were exposed to, the more likely they were to report lower levels of care (B = − 1.74,SE = 0.66,p = 0.03) and higher levels of denial of psychological autonomy by fathers (B = 1.05,SE = 0.45,p = 0.04). Maternal physical discipline was not significantly associated with children’s evaluation of their mothers’ parenting (ps ≥ 0.53).ConclusionsPhysical discipline was a common experience among our Singaporean sample, consistent with the notion that strict parenting could be regarded as a form of care. However, exposure to physical discipline did not translate to children reporting their parents as caring, with paternal physical discipline being negatively associated with children’s evaluations of paternal care.
Callous-unemotional traits and externalizing problem behaviors in left-behind preschool children: the role of emotional lability/negativity and positive teacher-child relationship
Authors: Tan Ruifeng , Guo Xinying , Chen Suiqing , He Guixian , Wu Xingtao 
Published: June 2023

AbstractBackgroundCallous-unemotional traits and emotional lability/negativity of young children have been regarded as the markers of externalizing problem behaviors. Based on the sensitivity to threat and affiliative reward model and the general aggression model, emotional lability/negativity may act as a mediator in the relationship between callous-unemotional traits and externalizing problem behaviors. Additionally, a positive teacher-child relationship could act as a buffer given the parental absence in left-behind children. However, these links remain unexplored in left-behind preschool children. Therefore, this study explored the link between callous-unemotional traits of left-behind preschool children and externalizing problem behaviors, as well as the mediating role of emotional lability/negativity and the moderating role of a positive teacher-child relationship.MethodData were collected on 525 left-behind children aged 3 to 6 years from rural kindergartens in China. Preschool teachers reported all data through an online survey platform. Moderated mediation analysis was performed to examine whether the mediated relation between callous-unemotional traits and externalizing problem behaviors was moderated by a positive teacher-child relationship.ResultsThe results showed callous-unemotional traits significantly predicted externalizing problem behaviors and lability/negativity acted as a mediator, while a positive teacher-child relationship acted as a protective factor in moderating the relationship between callous-unemotional traits and emotional lability/negativity. This study identified a moderated mediation effect among the four variables in left-behind preschool children in China.ConclusionThe findings provide support for the advancement of theoretical foundations, and provide an avenue for further exploration to support the mental health and overall development of left-behind children during early childhood.
The downregulation of miR-509-3p expression by collagen type XI alpha 1-regulated hypermethylation facilitates cancer progression and chemoresistance via the DNA methyltransferase 1/Small ubiquitin-like modifier-3 axis in ovarian cancer cells
Authors: Wu Yi-Hui , Huang Yu-Fang , Wu Pei-Ying , Chang Tzu-Hao , Huang Soon-Cen , Chou Cheng-Yang 
Published: June 2023

Abstract
Background
MicroRNAs are a group of small non-coding RNAs that are involved in development and diseases such as cancer. Previously, we demonstrated that miR-335 is crucial for preventing collagen type XI alpha 1 (COL11A1)-mediated epithelial ovarian cancer (EOC) progression and chemoresistance. Here, we examined the role of miR-509-3p in EOC.

Methods
The patients with EOC who underwent primary cytoreductive surgery and postoperative platinum-based chemotherapy were recruited. Their clinic-pathologic characteristics were collected, and disease-related survivals were determined. The COL11A1 and miR-509-3p mRNA expression levels of 161 ovarian tumors were determined by real-time reverse transcription-polymerase chain reaction. Additionally, miR-509-3p hypermethylation was evaluated by sequencing in these tumors. The A2780CP70 and OVCAR-8 cells transfected with miR-509-3p mimic, while the A2780 and OVCAR-3 cells transfected with miR-509-3p inhibitor. The A2780CP70 cells transfected with a small interference RNA of COL11A1, and the A2780 cells transfected with a COL11A1 expression plasmid. Site-directed mutagenesis, luciferase, and chromatin immunoprecipitation assays were performed in this study.

Results
Low miR-509-3p levels were correlated with disease progression, a poor survival, and high COL11A1 expression levels. In vivo studies reinforced these findings and indicated that the occurrence of invasive EOC cell phenotypes and resistance to cisplatin are decreased by miR-509-3p. The miR-509-3p promoter region (p278) is important for miR-509-3p transcription regulation via methylation. The miR-509-3p hypermethylation frequency was significantly higher in EOC tumors with a low miR-509-3p expression than in those with a high miR-509-3p expression. The patients with miR-509-3p hypermethylation had a significantly shorter overall survival (OS) than those without miR-509-3p hypermethylation. Mechanistic studies further indicated that miR-509-3p transcription was downregulated by COL11A1 through a DNA methyltransferase 1 (DNMT1) stability increase. Moreover, miR-509-3p targets small ubiquitin-like modifier (SUMO)-3 to regulate EOC cell growth, invasiveness, and chemosensitivity.

Conclusion
The miR-509-3p/DNMT1/SUMO-3 axis may be an ovarian cancer treatment target.
Identification of STEAP3-based molecular subtype and risk model in ovarian cancer
Authors: Zhao Zouyu , Sun Chongfeng , Hou Jishuai , Yu Panpan , Wei Yan , Bai Rui , Yang Ping 
Published: June 2023

AbstractBackgroundOvarian cancer (OC) is one of the most common malignancies in women. It has a poor prognosis owing to its recurrence and metastasis. Unfortunately, reliable markers for early diagnosis and prognosis of OC are lacking. Our research aimed to investigate the value of the six-transmembrane epithelial antigen of prostate family member 3 (STEAP3) as a prognostic predictor and therapeutic target in OC using bioinformatics analysis.MethodsSTEAP3 expression and clinical data were acquired from The Cancer Genome Atlas (TCGA), Genotype-Tissue Expression (GTEx), and Gene Expression Omnibus (GEO). Unsupervised clustering was used to identify molecular subtypes. Prognosis, tumor immune microenvironment (TIME), stemness indexes, and functional enrichment analysis were compared between two definite clusters. Through the least absolute shrinkage and selection operator (LASSO) regression analysis, a STEAP3-based risk model was developed, and the predictive effectiveness of this signature was confirmed using GEO datasets. A nomogram was used to predict the survival possibility of patients. Additionally, TIME, tumor immune dysfunction and exclusion (TIDE), stemness indexes, somatic mutations, and drug sensitivity were evaluated in different risk groups with OC. STEAP3 protein expression was detected using immunohistochemistry (IHC).ResultsSTEAP3 displayed marked overexpression in OC. STEAP3 is an independent risk factor for OC. Based on the mRNA levels of STEAP3-related genes (SRGs), two distinct clusters were identified. Patients in the cluster 2 (C2) subgroup had a considerably worse prognosis, higher immune cell infiltration, and lower stemness scores. Pathways involved in tumorigenesis and immunity were highly enriched in the C2 subgroup. A prognostic model based on 13 SRGs was further developed. Kaplan-Meier analysis indicated that the overall survival (OS) of high-risk patients was poor. The risk score was significantly associated with TIME, TIDE, stemness indexes, tumor mutation burden (TMB), immunotherapy response, and drug sensitivity. Finally, IHC revealed that STEAP3 protein expression was noticeably elevated in OC, and overexpression of STEAP3 predicted poor OS and relapse-free survival (RFS) of patients.ConclusionIn summary, this study revealed that STEAP3 reliably predicts patient prognosis and provides novel ideas for OC immunotherapy.
Isolated splenic peliosis: a case report
Authors: Nassr Bahaa , Hassan Wael Abdu , Nassr Hasan , Allouzi Abdullah , Al-Shebly Mohammed 
Published: June 2023

Abstract
Background
Peliosis is a rare condition with anatomopathological characteristics that affect the liver. However, splenic peliosis is even more unique and rare. Patients with such abnormality usually exhibit no symptoms. Moreover, this is a lethal condition due to the high probability of splenic rupture accompanied by shock.

Case presentation
We present a case of a 29-year-old Arab female who was admitted to the hospital with severe upper abdominal pain that started 1 week from the date of admission, associated with nausea, anorexia, low-grade fever, and vomiting, with no past medical history or comorbidities. A computerized tomography scan with contrast showed intraperitoneal free fluid along with multiple hypodense splenic cysts. Hence, an emergent exploratory laparotomy with splenectomy was performed. Splenic peliosis was confirmed by the histopathological examination.

Conclusion
Further investigations are warranted if peliosis is confirmed in one organ, for example, the liver, to detect its presence in any other potential organs that can be affected by peliosis. Splenic peliosis is extraordinarily rare. Furthermore, such a disease has no established management plan. Definitive treatment is surgical. Many aspects of splenic peliosis remain puzzling requiring more research in the near future.
Multiplicity of positive periodic solutions to third-order variable coefficients singular dynamical systems
Authors: Li Shengjun , Zhang Fang 
Published: June 2023

AbstractIn this paper, by applying a nonlinear alternative principle of Leray–Schauder and Guo–Krasnosel’skii fixed point theorem on compression and expansion of cones, together with truncation technique, we study the existence of multiplicity noncollision periodic solutions to third-order singular dynamical systems. By combining the analysis of the sign of Green’s function for a linear equation, we consider the systems where the potential has a repulsive singularity at origin. The so-called strong force condition is not needed, and the nonlinearity may have sign changing behavior. Recent results in the literature, even in the scalar case, are generalized and improved.
Adolescent peer support for mental health problems: evaluation of the validity and reliability of the Mental Health Support Scale for Adolescents
Authors: Lu Shurong , Hart Laura M. , Jorm Anthony F. , Gregg Karen , Gross Maxine , Mackinnon Andrew J. , Morgan Amy J. 
Published: June 2023

Abstract
Background
The Mental Health Support Scale for Adolescents (MHSSA) is a criterion-referenced measure of adolescents’ supportive intentions towards peers with mental health problems, which was developed for use in evaluations of adolescent mental health interventions, such as the teen Mental Health First Aid (tMHFA) program. The present study aimed to examine the validity and reliability of the MHSSA.

Methods
A sample of 3092 school students (Mean ± SD: 15.9 ± 0.4 years old) and 65 tMHFA Instructors (the adult group with known expertise in tMHFA) completed the 12 items of the MHSSA. A sub-sample of 1201 students repeated the scale after a 3-4-week interval. Item concordance rates with the tMHFA Action Plan across helpful and harmful intentions scales were calculated. Scale reliabilities were assessed using agreement coefficients from a single test administration and test-retest reliability measured by intraclass correlation coefficients. The mean differences of MHSSA scores of students and Instructors were compared using independent samples t-tests, while convergent validity was tested via correlations of the scale with validated measures of confidence in providing help, social distance and personal stigma.

Results
The average score of Instructors was significantly higher than that of students. The scale was positively associated with confidence in providing help, whilst negatively associated with social distance and dimensions of personal stigma. All scales of MHSSA had high agreement coefficients (all > 0.80) and fair to good test-retest reliability over 3–4 weeks.

Conclusions
The MHSSA shows evidence of validity and reliability for use among adolescents for evaluating the quality of intentions to help peers with mental health problems.
From ambient vibration data analysis to 1D ground-motion prediction of the Mj 5.9 and the Mj 6.5 Kumamoto earthquakes in the Kumamoto alluvial plain, Japan
Authors: Hailemikael Salomon , Di Giulio Giuseppe , Milana Giuliano , Vassallo Maurizio , Bordoni Paola 
Published: June 2023

AbstractWe present horizontal ground motion predictions at a soft site in the Kumamoto alluvial plain for the Mj 5.9 and Mj 6.5 Kumamoto earthquakes of April 2016, in the framework of an international blind prediction exercise. Such predictions were obtained by leveraging all available information which included: (i) analysis of earthquake ground motions; (ii) processing of ambient vibration data (AMV); and (iii) 1D ground response analysis. Spectral analysis of earthquake ground-motion data were used to obtain empirical estimates of the prediction site amplification function, with evidence of an amplification peak at about 1.2 Hz. Horizontal-to-vertical spectral ratio analysis of AMV confirmed this resonance frequency and pointed out also a low-frequency resonance around 0.3 Hz at the prediction site. AMV were then processed by cross-correlation, modified spatial autocorrelation and high-resolution beamforming methods to retrieve the 1D shear-wave velocity (Vs) structure at the prediction site by joint inversion of surface-wave dispersion and ellipticity curves. The use of low frequency dispersion curve and ellipticity data allowed to retrieve a reference Vs profile down to few thousand meters depth which was then used to perform 1D equivalent-linear simulations of the M 5.9 event, and both equivalent-linear and nonlinear simulations of the M 6.5 event at the target site. Adopting quantitative goodness-of-fit metrics based on time–frequency representation of the signals, we obtained fair-to-good agreement between 1D predictions and observations for the Mj 6.5 earthquake and a poor agreement for the Mj 5.9 earthquake. In terms of acceleration response spectra, while ground-motion overpredictions were obtained for the Mj 5.9 event, simulated ground motions for the Mj 6.5 earthquake severely underestimate the observations, especially those obtained by the nonlinear approach.
Graphical Abstract