bluuebunny/crossref_metadata_2025_split huggingface dataset:
A novel human specific lncRNA MEK6-AS1 regulates adipogenesis and fatty acid biosynthesis by stabilizing MEK6 mRNA
Authors: Li Di , Chen Yunhua , Zhu Xingyu , Yang Yanlei , Li Hongling , Zhao Robert Chunhua 
Published: January 2025

Abstract
Background
Obesity is becoming one of the major non-communicable diseases with increasing incidence and risks that cannot be ignored. However effective and safe clinical treatment strategies still need to be deeply explored. Increased number and volume of adipocytes lead to overweight and obesity. The aim of our work is to identify lncRNAs that have important regulatory in differentiation of human mesenchymal stem cells (MSCs) into adipocytes, and to provide effective targets for clinical prevention and treatment of obesity and related metabolic disorders.

Methods
We extracted primary MSCs from human adipose tissue, and conducted expression profile analysis of lncRNAs during adipogenic differentiation of MSCs to screen changed lncRNAs. Characteristics of lncRNA were revealed mainly by RACE and RNA FISH. Loss- and gain-of function experiments in vivo and in vitro were used to analyze effects of lncRNA. Targeted metabolomics was utilized to detect levels of free fatty acids. RNA pull-down, mRNA stability tests, etc. were employed to explore mechanisms of lncRNA.

Results
Human-specific lncRNA, we named it MEK6-AS1, was the most up-regulated transcript during adipogenic differentiation of MSCs. MEK6-AS1 was highly expressed in adipose tissue samples from individuals with BMI ≥ 25 and positively correlated with adipogenic marker genes in these samples. Knocking down lncRNA inhibited expression of adipogenic differentiation markers and ectopic adipogenesis, reducing contents of various free fatty acids, as well as promoting osteogenic differentiation. Overexpression of lncRNA had the opposite effects to the above processes. We also found that MEK6-AS1 was elevated during hepatic steatosis organoid generation. Mechanistically, MEK6-AS1 worked partially through stabilization of MEK6 mRNA by NAT10.

Conclusions
We have identified a human-specific lncRNA (MEK6-AS1) with position information in the genomic database but has not been extensively reported. We demonstrated that MEK6-AS1 as a novel lncRNA involved in adipogenic differentiation and adipogenesis, fatty acid metabolism, and osteogenic differentiation. We found that MEK6-AS1 may exert its effect by enhancing MEK6 mRNA stability through NAT10. Our study may provide insights into implication of lncRNAs in stem cell biology and offer a new potential therapeutic target for the prevention and treatment of obesity and other related disease.
Pyopericardium with cardiac tamponade caused by pyogenic liver abscess: a case report
Authors: Steffen Carolin , Sägmüller Josef , Schöneburg Dominique , Göncz Eva , Möckel Martin , Ott Sascha , Ungur Alexander Lavinius , O ’Brien Benjamin 
Published: January 2025

Abstract
Introduction
Purulent bacterial pericarditis is a potentially fatal disease with mortality rates reaching 100% if left untreated.

Case presentation
We present the case of a 33-year-old Caucasian male patient who developed cardiac tamponade, most likely caused by a pyogenic liver abscess communicating with the pericardium. Treatment with antibiotics, extended sepsis therapy, and drainage of the abscess led to a full recovery.

Conclusion
This report describes a rare but potentially fatal differential diagnosis of aortic dissection and serves as a reminder that lives abscesses can manifest unexpectedly. Clinical signs and symptoms of tamponade can be mistaken as sepsis. In this particular case, the combination of a septic abscess and tamponade caused by pyopericardium posed a diagnostic challenge.
A blockchain and internet of things-based information infrastructure for the Chinese automotive sector carbon-credit market
Authors: Liu Yanchu , Zhang Yu , Zheng Duosi 
Published: January 2025

AbstractIn 2021, 12 fraudulent cases were identified in the Chinese carbon market. As a critical component of this emerging market, China’s carbon-credit scheme in the automotive sector faces several shortcomings, including informational opacity and operational inefficiency, which affect market functionality and fairness. This study develops an information system that integrates blockchain technology and the Internet of Things to manage a carbon-credit scheme. Specifically, we attached carbon credits to each vehicle with radio frequency identification electronic tags and a chained data structure to ensure the traceability and reliability of information flow. We use the distributed ledger technology and establish five distinct types of smart contracts for decentralized operations to ensure that all procedures of the Chinese carbon-credit scheme are standardized and under public scrutiny. The proposed infrastructure has the potential to significantly enhance the transparency and efficiency of China’s carbon-credit schemes.
Effect of using a hemostatic gelatin sponge versus a dense polytetrafluorethylene membrane for socket sealing following immediate implant placement: a randomized clinical study
Authors: Shalash Mahmoud , Emara Aalaa 
Published: January 2025

Abstract
Background
Socket sealing following flapless immediate implant placement involves placing a barrier membrane to prevent the ingrowth of bacteria or contaminants to the dental implant during the early phase of healing. Dense Polytetrafluorethylene membranes have been the gold standard socket sealing barrier due to their impermeability to the oral flora, which allows for undisturbed implant healing. The search for a more cost-efficient material for sealing the socket without compromising implant osseointegration was sought. This study aims to evaluate the effectiveness of socket sealing after immediate implant placement in the premolar/molar region using a gelatin sponge, as opposed to a dense Polytetrafluorethylene membrane, and its impact on clinical implant osseointegration.

Materials and methods
The study was conducted on 20 patients, comprising 12 males and 8 females with an age range of 25–60 years. Those meeting the eligibility criteria of immediate implant placement were selected and divided equally into two groups. A control group, where an immediate implant and a dense polytetrafluorethylene membrane were used, and a test group where an immediate implant and a gelatin sponge was used. Exclusion criteria included smokers, presence of systemic or metabolic conditions, or a local pathology that would contraindicate implant placement. Teeth were extracted using a flapless approach. Implants were placed, and sockets were sealed using the allocated barrier. In the control group, the membrane was removed after 4 weeks. For the test group, the gelatin sponge was left to resorb spontaneously. After 4 months, implants were uncovered, and a 30 N/cm reverse torque test was applied to assess the clinical osseointegration.

Results
All implants healed uneventfully, without any radiographic evidence of bone loss. There was no clinical or radiographic difference between the outcomes in the control and the test groups. For the 20 dental implants subjected to a 30 N/cm reverse torque test, clinical integration was observed.

Conclusion
Within the limitations of this study, both dense polytetrafluorethylene membranes and gelatin sponges are effective methods for socket sealing, allowing for successful osseointegration following flapless immediate implant placement in posterior sites, with gelatin sponge offering easier handling and a reduced cost compared to dense polytetrafluorethylene. A larger study sample to verify the outcomes of this stud is recommended.
Endoplasmic reticulum stress in the salivary glands of patients with primary and associated Sjögren’s disease, and non-Sjögren’s sicca syndrome: a comparative analysis and the influence of chloroquine
Authors: Cavalcanti Graziela Vieira , de Oliveira Fabiola Reis , Bannitz Rafael Ferraz , de Paula Natalia Aparecida , Motta Ana Carolina Fragoso , Rocha Eduardo Melani , Chiorini John , Ricz Hilton Marcos Alves , Garcia Denny Marcos , Foss-Freitas Maria Cristina , de Freitas Luiz Carlos Conti 
Published: January 2025

Abstract

Background
Endoplasmic reticulum stress (ERS) and the unfolded protein response (UPR) are adaptive mechanisms for conditions of high protein demand, marked by an accumulation of misfolded proteins in the endoplasmic reticulum (ER). Rheumatic autoimmune diseases (RAD) are known to be associated with chronic inflammation and an ERS state. However, the activation of UPR signaling pathways is not completely understood in Sjögren’s disease (SD). This study evaluated the expression of ERS-related genes in glandular tissue of patients with primary SD (pSD), associated SD (aSD) with other autoimmune diseases, and non-Sjögren sicca syndrome (NSS).

Methods
In a cross-sectional study, minor salivary gland biopsies were obtained from 44 patients with suspected SD and 13 healthy controls (HC). Patients were classified as pSD, aSD, or NSS based on clinical, serological, and histological assessment. Histopathological analysis and mRNA expression analysis of genes associated with ERS and UPR (PERK, XBP1, ATF-6, ATF-4, CANX, CALR, CHOP, and BIP) were performed on the samples. Differences between groups (pSD, aSD, NSS, and HC) were assessed. The influence of chloroquine (CQ) on the ER was also investigated.

Results
Twenty-eight SD patients showed increased expression of PERK (p = 0.0117) and XBP1 (p = 0.0346), and reduced expression of ATF-6 (p = 0.0003) and CHOP (p = 0.0003), compared to the HC group. Increased expression of BIP (p < 0.0001), PERK (p = 0.0003), CALR (p < 0.0001), and CANX (p = 0.0111) was also observed in the SD group compared to the NSS group (n = 16). Patients receiving CQ (n = 16) showed a significant increase in ATF-6 (p = 0.0317) compared to patients not taking the medication (n = 29).

Conclusions
Altogether, the results suggest a greater activation of the ERS and UPR genes in patients with SD, especially in the pSD group. Antimalarial drugs, like CQ, used to treat RAD, may affect the ER function in exocrine glands.
Biventricular dysfunction predicts mortality in ST elevation myocardial infarction patients with cardiogenic shock
Authors: Prasetyo Angga Dwi , Bagaswoto Hendry Purnasidha , Saputra Firandi , Maharani Erika , Setianto Budi Yuli 
Published: January 2025

Abstract
Background
The incidence of mortality in patients with cardiogenic shock due to ST elevation myocardial infarction (STEMI) remains high even with prompt reperfusion therapy. Ventricular systolic dysfunction is the primary condition causing cardiogenic shock in STEMI. Studies have been widely conducted on the left ventricle (LV) and right ventricle (RV) systolic dysfunction related to mortality events. However, the parameters of biventricular systolic dysfunction predicting mortality as a stronger predictor of mortality are still unclear. Accordingly, we evaluated the predictor mortality value of biventricular systolic dysfunction in STEMI patients with cardiogenic shocks. Based on The Society for Cardiovascular Angiography and Intervention classification, we analyzed data from November 2021 to September 2023 at Dr. Sardjito General Hospital in Yogyakarta, Indonesia, using the Sardjito Cardiovascular Intensive Care (SCIENCE) registry with a retrospective cohort design. Multivariate logistic regression analysis was used to assess predictors of in-hospital mortality.

Results
There were 1,059 subjects with a mean ± SD age of 59 ± 11 years, dominated by men (80.5%) who met the inclusion and exclusion criteria. Based on multivariate analysis, biventricular dysfunction (BVD) is a factor that significantly increases the risk of in-hospital mortality (Odds ratio [OR], 1.771: 95% confidence interval [CI] 1.113–2.819; p = 0.016). Other significant factors affecting mortality were renal failure (OR, 5.122; 95% CI 3.233–8.116; p < 0.001), percutaneous coronary intervention (PCI) (OR, 0.493; 95% CI 0.248–0.981; p = 0.044), and inotropic/vasopressor (OR, 6.876; 95% CI 4.583–10.315; p < 0.001).

Conclusions
Biventricular dysfunction significantly increases the risk of in-hospital mortality in STEMI patients with cardiogenic shock. Renal failure, PCI, and the requirement for inotropic or vasopressor drugs are also factors that influence in-hospital mortality.
Long-term outcomes of Fontan palliation; the influence of the dominant ventricle
Authors: Helal Abdelmonem M. , Al-Ata Jameel , Badawy Nashwa Mostafa , Abdelwahed Ahmed , Ba-Atiyah Wejdan Khaled , Baeshen Anhar Ali , Alata Abdullah J. , Elmahrouk Ahmed F. , Shihata Mohammad S. , Jamjoom Ahmed A. , Bekheet Samia 
Published: January 2025

Abstract
Background
Factors affecting the outcomes after Fontan palliation are still controversial. Thus, this study aimed to compare hospital and long-term outcomes after Fontan palliation in patients with left-dominant, right-dominant, and co-dominant ventricles. Moreover, the study sought to identify factors associated with long-term surgical reintervention, catheter-based interventions, and morality. This retrospective cohort study was conducted in a single tertiary referral center between 2010 and 2024 and included all patients who had Fontan palliation (n = 247). The patients were grouped according to the dominant ventricle into three groups: left-dominant (n = 105), right-dominant (n = 108), and codominant (n = 36).

Results
No significant differences were found in demographic variables among groups. Heterotaxy was more common in codominant patients who exhibited higher preoperative saturation levels. Early postoperative complications were more frequent in right-dominant patients, with low cardiac output significantly lower in left-dominant patients. Codominant patients experienced more arrhythmias and complete heart block, while operative mortality rates were similar across groups. During a median follow-up of 80 months, the freedom from surgical reintervention at 5, 10, and 15 years was highest in the left-dominant group (97%) compared to right dominance (93%, 78%) and codominance (97%, 87%). Mortality was significantly higher in the right-dominant and codominant groups, with survival rates at 5, 10, and 15 years being 97% for left dominance, 92% for right dominance, and 91% for codominance. Factors influencing long-term mortality were older age, right dominance, and elevated pulmonary artery pressure.

Conclusions
Patients with left dominance exhibited superior survival rates and fewer complications compared to those with right dominance and codominance. The findings emphasize the importance of anatomical considerations in risk stratification and clinical decision-making. Young age at the time of palliation could improve the outcomes of the Fontan procedure.
Assessing ChatGPT's Capability in Addressing Thyroid Cancer Patient Queries: A Comprehensive Mixed-Methods Evaluation
Authors: Gorris Matthew A , Randle Reese W , Obermiller Corey S , Thomas Johnson , Toro-Tobon David , Dream Sophie Y , Fackelmayer Oliver J , Pandian T K , Mayson Sarah E 
Published: January 2025

Abstract

Context
Literature suggests patients with thyroid cancer have unmet informational needs in many aspects of care. Patients often turn to online resources for their health-related information, and generative artificial intelligence programs such as ChatGPT are an emerging and attractive resource for patients.

Objective
To assess the quality of ChatGPT's responses to thyroid cancer-related questions.

Methods
Four endocrinologists and 4 endocrine surgeons, all with expertise in thyroid cancer, evaluated the responses to 20 thyroid cancer-related questions. Responses were scored on a 7-point Likert scale in areas of accuracy, completeness, and overall satisfaction. Comments from the evaluators were aggregated and a qualitative analysis was performed.

Results
Overall, only 57%, 56%, and 52% of the responses “agreed” or “strongly agreed” that ChatGPT's answers were accurate, complete, and satisfactory, respectively. One hundred ninety-eight free-text comments were included in the qualitative analysis. The majority of comments were critical in nature. Several themes emerged, which included overemphasis of diet and iodine intake and its role in thyroid cancer, and incomplete or inaccurate information on risks of both thyroid surgery and radioactive iodine therapy.

Conclusion
Our study suggests that ChatGPT is not accurate or reliable enough at this time for unsupervised use as a patient information tool for thyroid cancer.
Point contact-restricted cAMP signaling controls ephrin-A5-induced axon repulsion
Authors: Bécret Johann , Gomez-Bravo Claudia , Michaud Camille , Assali Ahlem , Chenais Naïg A. L. , Kankadze Izeta , Roche Fiona , Couvet Sandrine , Fassier Coralie , Nicol Xavier 
Published: January 2025

ABSTRACT
Signal transduction downstream of axon guidance molecules is essential for steering developing axons. Second messengers including cAMP are key molecules shared by a multitude of signaling pathways and are required for a wide range of cellular processes including axon pathfinding. Yet, how these signaling molecules achieve specificity for each of their downstream pathways remains elusive. Subcellular compartmentation has emerged as a flexible strategy to reach such a specificity. Here, we show that point contact-restricted cAMP signals control ephrin-A5-evoked axon repulsion in vitro by modulating focal adhesion kinase (FAK; also known as PTK2) phosphorylation and the assembly and disassembly rate of point contacts. Consistent with this, preventing point contact-specific cAMP signals in developing retinal ganglion cells in vivo alters the refinement of their terminal axonal arbor in the brain. Altogether, our study identifies point contacts as a compartment containing a local cAMP signal required for ephrin-A5-dependent axon guidance and highlights the crucial role of such subcellularly restricted second messenger signals in the wiring of neuronal circuits.
Current guidelines for the management of Lyme borreliosis in European countries
Authors: Wyleciał Zuzanna , Ząber Julia , Zamachowska Wiktoria 
Published: January 2025

Introduction and purpose:
Lyme borreliosis, caused by Borrelia burgdorferi sensu lato complex, is the most common vector-borne disease in Europe. Transmitted by Ixodes ticks, its prevalence is increasing due to climate change, expanded tick habitats, and improved diagnostic awareness. Consequently, unified and evidence-based management guidelines are critical to ensure consistent diagnosis and treatment across European countries. This article provides an overview of current guidelines from 2023 for the management of Lyme borreliosis in Europe.

Materials and methods:
A literature search was conducted by analysing scientific articles published in Google Scholar, PubMed, and UpToDate using keywords included: Lyme disease; guidelines; Borrelia burgdorferi; management. We also searched current guidelines of Polish Society of Epidemiology and Infectious Diseases and recommendations from other European countries.
Description of the state of knowledge:
There are 3 stages of Lyme disease: early localized, early disseminated and late. Clinical diagnosis is based on symptoms, history of tick exposure, and observation of erythema migrans. To confirm Lyme disease, laboratory tests must also be performed. Lyme borreliosis is typically treated with antibiotics depending on the character.
Conclusion:
European guidelines for Lyme borreliosis emphasize early diagnosis and appropriate antibiotic therapy based on disease stage. Preventive measures and public health initiatives are essential to reduce the disease burden. While current guidelines ensure effective management, continued harmonization and advancements in diagnostics, treatment, and vaccine development will further enhance Lyme borreliosis care across Europe.
Keywords: Lyme disease; guidelines; Borrelia burgdorferi; management.
Récits d’Ellis Island:
Authors: Moraes Ana Lúcia 
Published: January 2025

O objetivo do presente trabalho é analisar a pertinência dos conceitos de Gilles Deleuze de “potência do falso” e de “fabulação”, tal como elaborados em sua obra Imagem-tempo: cinema II, para abordar a primeira parte do documentário Récits d’Ellis Island (Relatos d’Ellis Island) de Robert Bober e Georges Perec, realizado entre 1978 e 1980. A primeira parte do documentário, intitulada Traces (Rastros) evoca a chegada a Ellis Island de cerca de dezesseis milhões de emigrantes provenientes da Europa, de 1892 a 1954. O propósito central da análise é verificar em que medida, a partir dos procedimentos cinematográficos empregados pelos autores, o questionamento das fronteiras entre verdade e fabulação permitem caracterizar o documentário como um filme político.
Imágenes, testimonios y archivos:
Authors: Bevilacqua Gilda , Zylberman Lior 
Published: January 2025

Presentación. Revista Maracanan, n. 37 - "Imágenes, testimonios y archivos: la representación cinematográfica de genocidios, masacres y dictaduras", p. 11-21, sept.-dic. 2024.
60 anos do golpe de 1964 | Entrevista com Lucas Pedretti
Authors: Pedretti Lucas 
Published: January 2025

Lucas Pedretti, pesquisador e professor de História, reflete sobre os 60 anos do golpe de 1964, criticando a decisão do governo Lula de não realizar eventos oficiais para celebrar a data. Ele destaca a frustração da sociedade civil e acadêmica, que promoveu mais de 110 eventos de rememoração crítica. Pedretti observa a evolução historiográfica, que agora inclui grupos antes marginalizados, como favelados, indígenas e LBTQIA+. Ele avalia a situação dos arquivos da ditadura no Brasil, ressaltando a necessidade de conservação e maior acesso, especialmente aos acervos das polícias e empresas que também guardam importantes informações sobre as violências do regime. Sobre a Justiça de Transição, ele aponta que as iniciativas têm sido lentas e limitadas, com desafios de responsabilização e reconhecimento das vítimas. O desmonte das políticas de memória durante os governos de Bolsonaro refletiu um retrocesso alarmante. Pedretti discute a militarização atual, afirmando que a repressão estatal persiste, principalmente contra a população negra e periférica, enquanto a ascensão da extrema-direita tem ameaçado o pacto social estabelecido pós-ditadura. Ele conclui que é vital reavaliar a democracia e garantir a justiça social para evitar os erros do passado.