bluuebunny/crossref_metadata_2025_split huggingface dataset:
Development and characterization of a multimeric recombinant protein based on the spike protein receptor binding domain of SARS-CoV-2 that can neutralize virus infection
Authors: de Lima Veronica Aparecida , da Silva Ferreira Rodrigo , Vilela Oliva Maria Luiza , Andreata-Santos Robert , Ramos Janini Luiz Mario , Maricato Juliana Terzi , Akamatsu Milena Apetito , Ho Paulo Lee , Schenkman Sergio 
Published: February 2023

AbstractBackgroundThe SARS-CoV-2 virus, responsible for the COVID-19 pandemic, has four structural proteins and sixteen non-structural proteins. The S-protein is one of the structural proteins exposed on the surface of the virus and is the main target for producing neutralizing antibodies and vaccines. The S-protein forms a trimer that can bind the angiotensin-converting enzyme 2 (ACE2) through its receptor binding domain (RBD) for cell entry.MethodsWe stably expressed in a constitutive manner in HEK293 cells a new recombinant protein containing a signal sequence of immunoglobulin to produce an extended C-terminal portion of the RBD followed by a region responsible for the trimerization inducer of the bacteriophage T4, and a sequence of 6 histidines. The protein was produced and released in the culture supernatant of cells and was purified by Ni-agarose column and exclusion chromatography. It was then characterized by SDS-polyacrylamide gel and used as antigen to generate protective antibodies to inhibit ACE2 receptor interaction and virus entry into Vero cells.ResultsThe purified protein displayed a molecular mass of 135 kDa and with a secondary structure like the monomeric RBD. Electrophoresis analysis in SDS-polyacrylamide gel with and without reducing agents, and in the presence of crosslinkers indicated that it forms a multimeric structure composed of trimers and hexamers. The purified protein was able to bind the ACE2 receptor and generated high antibody titers in mice (1:10000), capable of inhibiting the binding of biotin labeled ACE2 to the virus S1 subunit, and to neutralize the entry of the SARS-CoV-2 Wuhan strain into cells.ConclusionOur results characterize a new multimeric protein based on S1 subunit to combat COVID-19, as a possible immunogen or antigen for diagnosis.
CRB3 navigates Rab11 trafficking vesicles to promote γTuRC assembly during ciliogenesis
Authors: Wang Bo , Liang Zheyong , tan Tan , Zhang Miao , Jiang Yina , Shang Yangyang , Gao Xiaoqian , Song Shaoran , Wang Ruiqi , Chen He , Liu Jie , Li Juan , Ren Yu , Liu Peijun 
Published: February 2023

AbstractThe primary cilium plays important roles in regulating cell differentiation, signal transduction, and tissue organization. Dysfunction of the primary cilium can lead to ciliopathies and cancer. The formation and organization of the primary cilium are highly associated with cell polarity proteins, such as the apical polarity protein CRB3. However, the molecular mechanisms by which CRB3 regulates ciliogenesis and the location of CRB3 remain unknown. Here, we show that CRB3, as a navigator, regulates vesicle trafficking in γ-TuRC assembly during ciliogenesis and cilium-related Hh and Wnt signaling pathways in tumorigenesis.Crb3knockout mice display severe defects of the primary cilium in the mammary ductal lumen and renal tubule, while mammary epithelial-specificCrb3knockout mice exhibit the promotion of ductal epithelial hyperplasia and tumorigenesis. CRB3 is essential for lumen formation and ciliary assembly in the mammary epithelium. We demonstrate that CRB3 localizes to the basal body and that CRB3 trafficking is mediated by Rab11-positive endosomes. Significantly, CRB3 interacts with Rab11 to navigate GCP6/Rab11 trafficking vesicles to CEP290, resulting in intact γ-TuRC assembly. In addition, CRB3-depleted cells are unresponsive to the activation of the Hh signaling pathway, while CRB3 regulates the Wnt signaling pathway. Therefore, our studies reveal the molecular mechanisms by which CRB3 recognizes Rab11-positive endosomes to facilitate ciliogenesis, and regulates cilium-related signaling pathways in tumorigenesis.
Scaling and controlling talent development in high-intensity organizations: the case of a Swedish football club
Authors: Carlsson-Wall Martin , DeMott Kai , Ali Hamza 
Published: February 2023

PurposeIn this paper, the authors empirically and theoretically analyze the scaling and control of talent development to highlight an important part of commercialization in football clubs, especially in the light of a growing transfer market.Design/methodology/approachConducting a single case study of a Swedish football club, the authors adapt a view of the club as a “high-intensity” organization (Alvesson and Kärreman, 2004), one that inherently relies on strong identification of employees and the fostering of talent. This view allows us to detail the importance of both socio-ideological and technocratic forms of control involved in the talent development process.FindingsThe authors show how socio-ideological and technocratic forms of control were combined to establish the football club as a “talent factory” in the league, as well as the corresponding challenges when scaling talent development activities and how these challenges were handled. In doing so, the authors contribute to the broader accounting literature on talent- and human resource management, as the authors provide an example of how football clubs may commercialize without necessarily violating their fundamental sports values.Originality/valueTalent management has mainly been studied in terms of increasing player wages and a focus on the cost of talent. As opposed to these perspectives, the authors highlight the revenue potential in developing players in the light of a growing transfer market and the relevance of talent development for the commercialization of football clubs.
Latent topics identification from the articles of Sri Lankan authors using LDA
Authors: Ravikumar S. , Boruah Bidyut Bikash , Gayang Fullstar Lamin 
Published: February 2023

Purpose
The purpose of the study is to identify the latent topics from 9102 Web of Science (WoS) indexed research articles published in 2645 journals of the Sri Lankan authors from 1989 to 2021 by applying Latent Dirichlet Allocation to the abstracts. Dominant topics in the corpus of text, the posterior probability of different terms in the topics and the publication proportions of the topics were discussed in the article.

Design/methodology/approach
Abstracts and other details of the studied articles are collected from WoS database by the authors. Data preprocessing is performed before the analysis. “ldatuning” from the R package is applied after preprocessing of text for deciding subjects in light of factual elements. Twenty topics are decided to extract as latent topics through four metrics methods.

Findings
It is observed that medical science, agriculture, research and development and chemistry-related topics dominate the subject categories as a whole. “Irrigation” and “mortality and health care” have a significant growth in the publication proportion from 2019 to 2021. For the most occurring latent topics, it is seen that terms like “activity” and “acid” carry higher posterior probability.

Practical implications
Topic models permit us to rapidly and efficiently address higher perspective inquiries without human mediation and are also helpful in information retrieval and document clustering. The unique feature of this study has highlighted how the growth of the universe of knowledge for a specific country can be studied using the LDA topic model.

Originality/value
This study will create an incentive for text analysis and information retrieval areas of research. The results of this paper gave an understanding of the writing development of the Sri Lankan authors in different subject spaces and over the period. Trends and intensity of publications from the Sri Lankan authors on different latent topics help to trace the interests and mostly practiced areas in different domains.
A bibliometric analysis and visualization of global research on rural livelihood
Authors: Khan Moin , Ahmad Ateeque , Yuvaraj Mayank , Husain Syed Shaz , Khan Daud 
Published: February 2023

Purpose
This study aims to explore the scientific research progress in the field of “Rural Livelihood” from 1991 to 2022.

Design/methodology/approach
A total of 2,122 documents were extracted from the Web of Science Core Collection using the key terms “Rural Livelihood” OR “Rural Livelihoods” in the “Title-Abstract-Keyword” field. The statistical analysis was performed through VOSviewer, Bibliometrix, Biblioshiney open-source package of R language environment, and MS Office software.

Findings
The results show that the first research article related to the field of rural livelihood was published in 1991, and the rapid growth in the publications of rural livelihood was observed since 2000, with a positive annual growth rate of 14.87%. Shackleton CM (n = 30 articles) and Belcher B (n = 1235 citations) are the most productive and highly cited authors in the field of rural livelihood, respectively. World Development is the most prolific and dominant journal, followed by Sustainability and Land Use Policy. The citation analysis disclosed that “Capitals and capabilities: a framework for analysing peasant viability, rural livelihoods and poverty” is the most cited research paper published in the field of rural livelihood by Anthony Bebbington. University of Copenhagen, Wageningen University and Rhodes University emerged as the topmost organizations engaged in rural livelihood research. The USA and the UK are the most productive and cited countries in rural livelihood. The keyword analysis revealed that most of the research published in the field of rural livelihood has focussed on rural livelihoods, management, conservation, poverty, strategies, climate change, etc.; however, the least emphasis is given on the subjects like food security, income diversification, biodiversity, deforestation, soil fertility management, bio-economy and environmental intervention. The thematic evolution reflects that the field of rural livelihood has been extensively researched and has undergone many dimensions, such as agriculture, management, conservation, climate change, households, policy and biodiversity.

Originality/value
The study’s findings provide an insight into global research trends, latest advances, hot issues, leading topics, and the thematic evolution of rural livelihood research over the last 31 years approximately. This study is quite useful to researchers and stakeholders to obtain rigorous bibliographic knowledge on literature related to the topic and work accordingly for R&D activities.
Information literacy development and competencies of high school students in Accra
Authors: Kankam Philip Kwaku 
Published: February 2023

Purpose
Information literacy (IL) is clearly important for academic performance, as evidenced by literature. It could be defined as a set of abilities, attitudes and experiences that enable people to recognize when they need information to solve an issue. The importance of investigating students’ IL competencies cannot be overstated. This study therefore aims to look into the IL development and competencies of high school students in Accra, as there appears to be a dearth of systematic study on this in Ghana.

Design/methodology/approach
To investigate this phenomenon, the study used a survey research design with a mixed-methods approach and a post-positivist research paradigm. A total of 454 high school students, 3 librarians and 3 heads of ICT departments from three senior high schools in Accra participated in this study. This study used two methods: an audit of the IL programmes and practices available at the selected schools as well as IL literacy assessment through the use of a standardized test instrument. The data collection tools used were a semi-structured interview schedule and a questionnaire.

Findings
This study found that high school students in Accra had low IL competencies. Again, the findings of this study revealed that inadequate infrastructure and lack of formalized IL instructions in schools hindered the IL development of students.

Originality/value
The author considers the study original both in conceptualization and design. The main question being interrogated stems from identified gaps in the literature and this study intends to fill these knowledge gaps. This study’s originality also stems from the fact that there is a paucity of information on the subject of study in the context of Ghana. This study recommends the need to integrate IL in the school curriculum to ensure effective and efficient IL instructions in high schools.
Cooperation and innovation under production risk: evidence from a lab-in-the-field experiment
Authors: Gómez Karoll , Arango Aramburo Santiago , Restrepo Soto Daniel 
Published: February 2023

PurposeThis study analyzes the role of cooperative behavior in facing the risk of encouraging innovative agricultural production projects by small-scale farmers in the context of farmers in developing countries.Design/methodology/approachA within-subjects field experiment was conducted with small-scale Colombian panela cane farmers. The authors used the collected data to run the regression analyses.FindingsThe results suggest that when small farmers can follow cooperative behavior by joining a group and pooling resources to face risk, they are more willing to invest in a novel and profitable alternative, albeit riskier. However, the possibility of cooperating with a group to invest in a novel production project depends on its expected risk level.Research limitations/implicationsThese results will help develop agricultural policies for sustainable development. Establishing informal networks for small-scale farmers to deal with unpredictable risks may aid in developing innovative systems.Social implicationsAgriculture is highly vulnerable to climatic impacts, which, combined with the inherent risk of innovation, may reduce small farmers' willingness to adopt innovation. Cooperation appears to be a mechanism for pooling resources and facing risk.Originality/valueResearch has focused on experimentally testing the effect of cooperative behavior when facing risk. The authors contribute to the literature by demonstrating the impact of the ability of small-scale farmers in rural areas to collectively manage risk on investment in innovative projects.
Integrating Industry 4.0 and agile new product development practices to evaluate the penetration of sustainable development goals in manufacturing industries
Authors: Palsodkar Manoj , Yadav Gunjan , Nagare Madhukar R. 
Published: February 2023

Purpose
The United Nations member countries adopted a set of 17 sustainable development goals (SDGs) to achieve a better and more sustainable future for all. It encourages the use of sustainable practices during new product development (NPD). Competitiveness has put pressure on organizations to maintain their market share and look for new approaches related to NPD. The current study aims to focus on creating a framework that can help to achieve the SDGs by adopting agile new product development (ANPD) practices and Industry 4.0 technologies.

Design/methodology/approach
From the literature, various ANPD practices, Industry 4.0 technologies, performance metrics, their interconnection and their contribution toward achieving SDGs are extracted. The weights of selected Industry 4.0–ANPD practices are computed by robust best worst method (RBWM), and the Fuzzy-VIKOR method is used to rank the selected performance metrics. To test the robustness of the developed framework, sensitivity analysis is also performed.

Findings
The results show that among the various Industry 4.0–ANPD practices “Multi-skilled employees” have the highest weight followed by “Customer requirement analysis and prioritization.” Whereas for performance metrics, “The number of innovative products launched per year” is ranked first, with the “Average time between two launches” at second place.

Practical implications
This research contributes to the adoption of ANPD practices and Industry 4.0 technologies for the achievement of the business SDGs. The shortlisted Industry 4.0–ANPD practices will help in resolving the social and environmental issues. The set of performance metrics will help practitioners and managers to evaluate the performance of ANPD in the context of business SDGs.

Originality/value
This study adds to the understanding related to Industry 4.0–ANPD practices adoption. And to the best of the authors’ knowledge, it is believed that no similar work has been done previously and by using industry insights into technology components, this work contributes to valuable insights into the subject.
Spurring entrepreneurship with public venture capital in developing industries – evidence from Hungary
Authors: Becsky-Nagy Patrícia , Fazekas Balázs 
Published: February 2023

Purpose
Venture capital (VC) is an essential element in healthy entrepreneurial environments; therefore, many countries in developing entrepreneurial economies support the industry via direct or indirect government interventions. The purpose of this study is to examine through the example of the Hungarian market, whether direct or hybrid state involvement has contributed more to the growth of the invested enterprises. The findings are relevant in the design of government VC schemes and in the contracts mitigating the moral hazards inherent in government funding.

Design/methodology/approach
The basis of empirical research is a unique hand-collected database covering Hungarian government-backed VC (GVC) investments. Based on the financial data of investee firms, the authors investigate whether firms financed by hybrid VC involving market participants are able to outperform firms that receive pure public financing using panel regression.

Findings
Based on Hungarian evidence, hybrid VC-backed firms generated lower growth and employment than their purely government-backed peers. Both schemes showed meagre innovation activity. The conclusion is that because of the conflict of private and economic policy objectives in hybrid financing, the exposure of hybrid risk capital to moral hazard is higher than that of pure public financing. Private interests in hybrid funds can only improve investment efficiency if they are structured along the lines of market-based independent financial intermediation and the contracts imitate the ones existing amongst limited and general partners in private schemes.

Research limitations/implications
The research covers the data of Hungarian government-backed firms by tracking the full range of 86 investments made in the purely government scheme and 340 firms that received funding in the hybrid scheme. The research focuses on two government initiatives, and the results are influenced by the specific regulation of the programs; therefore, the results cannot be generalized for all government agendas; they are indicative in the designs of the agendas.

Originality/value
There is a limited number of empirical studies investigating the impact of VC in developing markets, especially in the Central and Eastern Europe region. This firm-level research on the impact of public VC can help improve the effectiveness of development policies. By analysing the entirety of investments of a VC program that is near to its completion, the authors provide new insight into the efficiency and prospects of GVC schemes in the region.
Lessons on employees' digital resilience from COVID-19-induced transitions to remote work – a mixed methods study
Authors: Kohn Vanessa , Frank Muriel , Holten Roland 
Published: February 2023

PurposeIn response to the COVID-19 pandemic, many employees had to switch to remote work. While some adjusted successfully to this transition, others have struggled. Leveraging information systems (IS) to adjust to major exogenous shocks is called digital resilience. The purpose of this paper is to understand what we can learn about employees' digital resilience from externally enforced transitions to remote work.Design/methodology/approachAs digital resilience is challenging to measure, this study uses an embedded mixed methods approach. The authors conducted a qualitative analysis of 40 employees' statements on their remote work experience during the first six months of the pandemic and complemented these findings with scale-based digital resilience scores.FindingsThe authors find that employees' digital resilience largely depends on the amount of technical equipment and support they receive from their organizations as well as their ability and willingness to learn how to adequately use and communicate through information and communication technologies. Being self-disciplined and self-responsible positively affects digital resilience, while social isolation threatens it. Organizations can foster digital resilience building by encouraging digital networking, building a digital culture and netiquette, and treating digital resilience as a sociotechnical phenomenon.Originality/valueThis is one of the first empirical studies of digital resilience on a human level. It sheds light on the missing link between IS-enabled resilience and transitions to remote work. Specifically, it provides original insights into its development and manifestation in a remote work context during the COVID-19 pandemic. For researchers, it provides novel guidance on choosing appropriate measurement instruments to capture digital resilience.
The moderating and mediating effects of corporate governance and capital structure on firm performance: empirical evidence from an emerging market
Authors: Ronoowah Rishi Kapoor , Seetanah Boopen 
Published: February 2023

PurposeThis study aims to focus on the direct, mediating and moderating effects of corporate governance (CG) and capital structure (CS) in their relationships with firm performance (FP).Design/methodology/approachMultivariate panel data regression techniques are employed to analyse the direct, mediating and moderating impacts of the CG and CS on FP of 38 listed Mauritian non-financial companies from 2009 to 2019.FindingsThis study shows that CG has a positive but insignificant influence on return on equity (ROE) and Tobin's Q. CS has a significant negative impact on both ROE and Tobin's Q and supports the pecking order theory (POT). The interaction of CG and CS influences FP, but the strength of the moderating effects depends on the performance measure being used. Both CS and CG have no mediation effects in their relationship with FP measured by ROE and Tobin's Q.Practical implicationsThe results indicate that the combination of the high leverage ratio and good governance practices of companies can improve FP and increases investor confidence resulting in a positive reaction on their market share prices.Originality/valueThis paper contributes to the CG and CS literature by introducing a more precise and comprehensive research approach and is the first to attempt to extend CG and CS in their associations with FP by incorporating both CG and CS as profound moderator and mediator variables simultaneously in the same study.