bluuebunny/crossref_metadata_2025_split huggingface dataset:
Decoding of human identity by computer vision and neuronal vision
Authors: Zhang Yipeng , Aghajan Zahra M. , Ison Matias , Lu Qiujing , Tang Hanlin , Kalender Guldamla , Monsoor Tonmoy , Zheng Jie , Kreiman Gabriel , Roychowdhury Vwani , Fried Itzhak 
Published: January 2023

AbstractExtracting meaning from a dynamic and variable flow of incoming information is a major goal of both natural and artificial intelligence. Computer vision (CV) guided by deep learning (DL) has made significant strides in recognizing a specific identity despite highly variable attributes. This is the same challenge faced by the nervous system and partially addressed by the concept cells—neurons exhibiting selective firing in response to specific persons/places, described in the human medial temporal lobe (MTL) ⁠. Yet, access to neurons representing a particular concept is limited due to these neurons’ sparse coding. It is conceivable, however, that the information required for such decoding is present in relatively small neuronal populations. To evaluate how well neuronal populations encode identity information in natural settings, we recorded neuronal activity from multiple brain regions of nine neurosurgical epilepsy patients implanted with depth electrodes, while the subjects watched an episode of the TV series “24”. First, we devised a minimally supervised CV algorithm (with comparable performance against manually-labeled data) to detect the most prevalent characters (above 1% overall appearance) in each frame. Next, we implemented DL models that used the time-varying population neural data as inputs and decoded the visual presence of the four main characters throughout the episode. This methodology allowed us to compare “computer vision” with “neuronal vision”—footprints associated with each character present in the activity of a subset of neurons—and identify the brain regions that contributed to this decoding process. We then tested the DL models during a recognition memory task following movie viewing where subjects were asked to recognize clip segments from the presented episode. DL model activations were not only modulated by the presence of the corresponding characters but also by participants’ subjective memory of whether they had seen the clip segment, and by the associative strengths of the characters in the narrative plot. The described approach can offer novel ways to probe the representation of concepts in time-evolving dynamic behavioral tasks. Further, the results suggest that the information required to robustly decode concepts is present in the population activity of only tens of neurons even in brain regions beyond MTL.
Comparative metabolic profiling of olive leaf extracts from twelve different cultivars collected in both fruiting and flowering seasons
Authors: Kabbash Eman M. , Abdel-Shakour Zeinab T. , El-Ahmady Sherweit H. , Wink Michael , Ayoub Iriny M. 
Published: January 2023

AbstractOlea europaea is an economically significant crop native to Mediterranean countries. Its leaves exhibit several biological properties associated to their chemical composition. The aqueous ethanolic extracts of olive leaves from twelve different cultivars were analyzed by high performance liquid chromatography coupled to photodiode array and electrospray ionization mass spectrometry (HPLC/PDA/ESI–MS/MS). A total of 49 phytochemicals were identified in both positive and negative ionization modes. The identified compounds belonged to four classes of secondary metabolites including secoiridoids, flavonoids, pentacyclic triterpenoids and various phenolic compounds. Seasonal variation in chemical composition among the studied cultivars was apparent in autumn and spring. Secologanoside, oleuropein, hydroxy-oleuropein, demethyl oleuropein, gallocatechin, luteolin-O-hexoside, diosmetin, oleanolic acid and maslinic acid were detected in all cultivars in both seasons. Oleuropein-O-deoxyhexoside was tentatively identified for the first time in olive leaf extracts; detected only in the Spanish cultivar Picual (PIC) collected in spring. Also, dihydroxy-oxooleanenoic acid and hydroxy-oxooleanenoic acid, two bioactive pentacyclic triterpenes, were identified. Principle component analysis (PCA) showed good discrimination among the studied cultivars in terms of their botanical origin. This study is considered the first study for non-targeted metabolic profiling of different olive leaf cultivars cultivated in Egypt.
Potentially toxic elements in the brains of people with multiple sclerosis
Authors: Pamphlett Roger , Buckland Michael E. , Bishop David P. 
Published: January 2023

AbstractPotentially toxic elements such as lead and aluminium have been proposed to play a role in the pathogenesis of multiple sclerosis (MS), since their neurotoxic mechanisms mimic many of the pathogenetic processes in MS. We therefore examined the distribution of several potentially toxic elements in the autopsied brains of people with and without MS, using two methods of elemental bio-imaging. Toxicants detected in the locus ceruleus were used as indicators of past exposures. Autometallography of paraffin sections from multiple brain regions of 21 MS patients and 109 controls detected inorganic mercury, silver, or bismuth in many locus ceruleus neurons of both groups, and in widespread blood vessels, oligodendrocytes, astrocytes, and neurons of four MS patients and one control. Laser ablation-inductively coupled plasma-mass spectrometry imaging of pons paraffin sections from all MS patients and 12 controls showed that combinations of iron, silver, lead, aluminium, mercury, nickel, and bismuth were present more often in the locus ceruleus of MS patients and were located predominantly in white matter tracts. Based on these results, we propose that metal toxicants in locus ceruleus neurons weaken the blood–brain barrier, enabling multiple interacting toxicants to pass through blood vessels and enter astrocytes and oligodendroglia, leading to demyelination.
Nutritional epigenomic and DNA-damage modulation effect of natural stilbenoids
Authors: Volpes Sara , Cruciata Ilenia , Ceraulo Federica , Schimmenti Chiara , Naselli Flores , Pinna Cecilia , Mauro Maurizio , Picone Pasquale , Dallavalle Sabrina , Nuzzo Domenico , Pinto Andrea , Caradonna Fabio 
Published: January 2023

AbstractThe aim of the present work is the evaluation of biological effects of natural stilbenoids found in Vitis vinifera, with a focus on their activity as epigenetic modulators. In the present study, resveratrol, pterostilbene and for the first time their dimers (±)-trans-δ-viniferin, (±)-trans-pterostilbene dehydrodimer were evaluated in Caco-2 and HepG-2 cell lines as potential epigenetic modulators. Stilbenoids were added in a Caco-2 cell culture as a model of the intestinal epithelial barrier and in the HepG-2 as a model of hepatic environment, to verify their dose-dependent toxicity, ability to interact with DNA, and epigenomic action. Resveratrol, pterostilbene, and (±)-trans-pterostilbene dehydrodimer were found to have no toxic effects at tested concentration and were effective in reversing arsenic damage in Caco-2 cell lines. (±)-trans-δ-viniferin showed epigenomic activity, but further studies are needed to clarify its mode of action.
Prediction of type 2 diabetes mellitus using hematological factors based on machine learning approaches: a cohort study analysis
Authors: Mansoori Amin , Sahranavard Toktam , Hosseini Zeinab Sadat , Soflaei Sara Saffar , Emrani Negar , Nazar Eisa , Gharizadeh Melika , Khorasanchi Zahra , Effati Sohrab , Ghamsary Mark , Ferns Gordon , Esmaily Habibollah , Mobarhan Majid Ghayour 
Published: January 2023

AbstractType 2 Diabetes Mellitus (T2DM) is a significant public health problem globally. The diagnosis and management of diabetes are critical to reduce the diabetes complications including cardiovascular disease and cancer. This study was designed to assess the potential association between T2DM and routinely measured hematological parameters. This study was a subsample of 9000 adults aged 35–65 years recruited as part of Mashhad stroke and heart atherosclerotic disorder (MASHAD) cohort study. Machine learning techniques including logistic regression (LR), decision tree (DT) and bootstrap forest (BF) algorithms were applied to analyze data. All data analyses were performed using SPSS version 22 and SAS JMP Pro version 13 at a significant level of 0.05. Based on the performance indices, the BF model gave high accuracy, precision, specificity, and AUC. Previous studies suggested the positive relationship of triglyceride-glucose (TyG) index with T2DM, so we considered the association of TyG index with hematological factors. We found this association was aligned with their results regarding T2DM, except MCHC. The most effective factors in the BF model were age and WBC (white blood cell). The BF model represented a better performance to predict T2DM. Our model provides valuable information to predict T2DM like age and WBC.
Converging orifice used to control the discharge rate of spherical particles from a flat floor silo
Authors: Wiącek Joanna , Horabik Józef , Molenda Marek , Parafiniuk Piotr , Bańda Maciej , Stasiak Mateusz 
Published: January 2023

AbstractThe effect of the converging orifice geometry in a model silo on the discharge rate of monosized spherical particles was studied experimentally and numerically. The cylindrical container was equipped with interchangeable inserts with converging discharge orifices of various upper diameters in the upper base and a constant lower diameter in the lower base. Plastic PLA beads and agricultural granular materials: wheat, rapeseeds, and linseeds were tested. A series of discrete element method simulations corresponding to the performed experiments was conducted with a largely extended set of experimental discharge conditions. In the case of the constant thickness of the insert, the discharge rate initially increased with an increase in the half cone angle of the converging orifice and then the tendency reversed. In the majority of cases, the discharge rate through the converging orifice was higher than through the hopper with the same orifice diameter.
Trogocytosis of neurons and glial cells by microglia in a healthy adult macaque retina
Authors: Goyal Megan , Bordt Andrea S. , Neitz Jay , Marshak David W. 
Published: January 2023

AbstractMicroglial cells are the primary resident immune cells in the retina. In healthy adults, they are ramified; that is, they have extensive processes that move continually. In adult retinas, microglia maintain the normal structure and function of neurons and other glial cells, but the mechanism underlying this process is not well-understood. In the mouse hippocampus, microglia engulf small pieces of axons and presynaptic terminals via a process called trogocytosis. Here we report that microglia in the adult macaque retina also engulf pieces of neurons and glial cells, but not at sites of synapses. We analyzed microglia in a volume of serial, ultrathin sections of central macaque retina in which many neurons that ramify in the inner plexiform layer (IPL) had been reconstructed previously. We surveyed the IPL and identified the somas of microglia by their small size and scant cytoplasm. We then reconstructed the microglia and studied their interactions with other cells. We found that ramified microglia frequently ingested small pieces of each major type of inner retinal neuron and Müller glial cells via trogocytosis. There were a few instances where the interactions took place near synapses, but the synapses, themselves, were never engulfed. If trogocytosis by retinal microglia plays a role in synaptic remodeling, it was not apparent from the ultrastructure. Instead, we propose that trogocytosis enables these microglia to present antigens derived from normal inner retinal cells and, when activated, they would promote antigen-specific tolerance.
Large-scale genomic analysis of Mycobacterium tuberculosis reveals extent of target and compensatory mutations linked to multi-drug resistant tuberculosis
Authors: Napier Gary , Campino Susana , Phelan Jody E. , Clark Taane G. 
Published: January 2023

AbstractResistance to isoniazid (INH) and rifampicin (RIF) first-line drugs in Mycobacterium tuberculosis (Mtb), together called multi-drug resistance, threatens tuberculosis control. Resistance mutations in katG (for INH) and rpoB (RIF) genes often come with fitness costs. To overcome these costs, Mtb compensatory mutations have arisen in rpoC/rpoA (RIF) and ahpC (INH) loci. By leveraging the presence of known compensatory mutations, we aimed to detect novel resistance mutations occurring in INH and RIF target genes. Across ~ 32 k Mtb isolates with whole genome sequencing (WGS) data, there were 6262 (35.7%) with INH and 5435 (30.7%) with RIF phenotypic resistance. Known mutations in katG and rpoB explained ~ 99% of resistance. However, 188 (0.6%) isolates had ahpC compensatory mutations with no known resistance mutations in katG, leading to the identification of 31 putative resistance mutations in katG, each observed in at least 3 isolates. These putative katG mutations can co-occur with other INH variants (e.g., katG-Ser315Thr, fabG1 mutations). For RIF, there were no isolates with rpoC/rpoA compensatory mutations and unknown resistance mutations. Overall, using WGS data we identified putative resistance markers for INH that could be used for genotypic drug-resistance profiling. Establishing the complete repertoire of Mtb resistance mutations will assist the clinical management of tuberculosis.
Cortical inhibitory but not excitatory synaptic transmission and circuit refinement are altered after the deletion of NMDA receptors during early development
Authors: Deng Rongkang , Chang Minzi , Kao Joseph P. Y. , Kanold Patrick O. 
Published: January 2023

AbstractNeurons in the cerebral cortex form excitatory and inhibitory circuits with specific laminar locations. The mechanisms underlying the development of these spatially specific circuits is not fully understood. To test if postsynaptic N-methyl-D-aspartate (NMDA) receptors on excitatory neurons are required for the development of specific circuits to these neurons, we genetically ablated NMDA receptors from a subset of excitatory neurons in the temporal association cortex (TeA) through in utero electroporation and assessed the intracortical circuits connecting to L5 neurons through in vitro whole-cell patch clamp recordings coupled with laser-scanning photostimulation (LSPS). In NMDAR knockout neurons, α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) receptor-mediated connections were largely intact. In contrast both LSPS and mini-IPSC recordings revealed that γ-aminobutyric acid type A (GABAA) receptor-mediated connections were impaired in NMDAR knockout neurons. These results suggest that postsynaptic NMDA receptors are important for the development of GABAergic circuits.
A non-catalytic N-terminus domain of WRN prevents mitotic telomere deprotection
Authors: Romero-Zamora Diana , Hayashi Makoto T. 
Published: January 2023

AbstractTelomeric ends form a loop structure (T-loop) necessary for the repression of ATM kinase activation throughout the normal cell cycle. However, cells undergoing a prolonged mitotic arrest are prone to lose the T-loop, resulting in Aurora B kinase-dependent mitotic telomere deprotection, which was proposed as an anti-tumor mechanism that eliminates precancerous cells from the population. The mechanism of mitotic telomere deprotection has not been elucidated. Here, we show that WRN, a RECQ helicase family member, can suppress mitotic telomere deprotection independently of its exonuclease and helicase activities. Truncation of WRN revealed that N-terminus amino acids 168–333, a region that contains a coiled-coil motif, is sufficient to suppress mitotic telomere deprotection without affecting both mitotic Aurora B-dependent spindle checkpoint and ATM kinase activity. The suppressive activity of the WRN168–333 fragment is diminished in cells partially depleted of TRF2, while WRN is required for complete suppression of mitotic telomere deprotection by TRF2 overexpression. Finally, we found that phosphomimetic but not alanine mutations of putative Aurora B target sites in the WRN168–333 fragment abolished its suppressive effect. Our findings reveal a non-enzymatic function of WRN, which may be regulated by phosphorylation in cells undergoing mitotic arrest. We propose that WRN enhances the protective function of TRF2 to counteract the hypothetical pathway that resolves the mitotic T-loop.
How priming with body odors affects decision speeds in consumer behavior
Authors: Alcañiz Mariano , Giglioli Irene Alice Chicchi , Carrasco-Ribelles Lucia A. , Minissi Maria Eleonora , López Cristina Gil , Semin Gün R. 
Published: January 2023

AbstractTo date, odor research has primarily focused on the behavioral effects of common odors on consumer perception and choices. We report a study that examines, for the first time, the effects of human body odor cues on consumer purchase behaviors. The influence of human chemosignals produced in three conditions, namely happiness, fear, a relaxed condition (rest), and a control condition (no odor), were examined on willingness to pay (WTP) judgments across various products. We focused on the speed with which participants reached such decisions. The central finding revealed that participants exposed to human odors reached decisions significantly faster than the no odor control group. The main driving force is that human body odors activate the presence of others during decision-making. This, in turn, affects response speed. The broader implications of this finding for consumer behavior are discussed.
Cu3Sn joint based on transient liquid phase bonding of Cu@Cu6Sn5 core–shell particles
Authors: Wang Jintao , Wang Jianqiang , Duan Fangcheng , Chen Hongtao 
Published: January 2023

AbstractWith the development of high-integration and high-power electronics, the lack of matching chip connecting materials that can withstand high temperatures has been a challenge. In this manuscript, a Cu@Cu6Sn5 core–shell bimetallic particles (approx. 1 μm in diameter) are successfully prepared and introduced as a new solder material for the packaging of power devices to obtain a Cu3Sn all-IMC solder joint. The joint consisted mainly of equiaxed Cu3Sn grains, and a small portion of columnar Cu3Sn grains. In columnar-type growth, Sn is the dominant diffusing species, which comes from the depletion of Sn in Cu6Sn5. The depleted Cu6Sn5 is transformed into columnar Cu3Sn. In equiaxed-type growth, Cu is the dominant diffusing species. Cu reacts with Cu6Sn5 to grow a Cu3Sn layer. This conclusion was confirmed by the orientation relationship. The equiaxed Cu3Sn grain nucleates at the Cu/Cu3Sn interface have an orientation relationship with the Cu substrate. Columnar Cu3Sn grains at the Cu6Sn5/Cu3Sn interface have an orientation relationship with Cu6Sn5.
A geospatial approach to understanding clean cooking challenges in sub-Saharan Africa
Authors: Khavari Babak , Ramirez Camilo , Jeuland Marc , Fuso Nerini Francesco 
Published: January 2023

AbstractUniversal clean cooking is a key target under Sustainable Development Goal (SDG) 7, with implications for several other SDGs, such as good health, gender equality and climate. Yet, 2.4 billion people globally still lack access to clean cooking. The situation is especially dire in sub-Saharan Africa (SSA), where only 17% use clean options. We develop OnStove, an open-source spatial tool comparing the relative potential of different cookstoves on the basis of their costs and benefits, and apply it to SSA. Our results suggest a severe market failure as the currently most used solution, traditional biomass, produces the lowest social net-benefits nearly everywhere in SSA. Correcting this failure, which stems from multiple market and behavioural obstacles, would deliver significant health, time and emission benefits but requires identification and promotion of policies to transform cooking energy use. Spatial mapping offers a more nuanced understanding of the costs needed to deliver cleaner cooking transitions than was previously possible, which is useful for improved targeting of intervention strategies.
Maternal intermittent fasting in mice disrupts the intestinal barrier leading to metabolic disorder in adult offspring
Authors: Liang Yuan , Yin Wenzhen , Luo Chao , Sun Lijun , Feng Tiange , Zhang Yunhua , Yin Yue , Zhang Weizhen 
Published: January 2023

AbstractMaternal nutrition plays a critical role in energy metabolism of offspring. We aim to elucidate the effect of long-term intermittent fasting (IF) before pregnancy on health outcomes of offspring. Here we show long-term IF before pregnancy disrupts intestinal homeostasis of offspring with subsequent disorder of glucose and lipid metabolism. This occurs through the reduction in beneficial microbiota such asLactobacillus_intestinalis. Our observations further support the concept that intestinal microbiota in offspring is vulnerable to maternal nutrition, and its homeostasis is critical for the integrity of intestinal barrier and metabolic homeostasis.
Similar adaptative mechanism but divergent demographic history of four sympatric desert rodents in Eurasian inland
Authors: Cheng Jilong , Peng Xingwen , Li Hong , Feijó Anderson , Xia Lin , Shenbrot Georgy I. , Ge Deyan , Wen Zhixin , Wang Dehua , Yang Qisen 
Published: January 2023

AbstractPhenotypes associated with metabolism and water retention are thought to be key to the adaptation of desert species. However, knowledge on the genetic changes and selective regimes on the similar and divergent ways to desert adaptation in sympatric and phylogenetically close desert organisms remains limited. Here, we generate a chromosome level genome assembly for Northern three-toed jerboa (Dipus sagitta) and three other high-quality genome assemblies for Siberian jerboa (Orientallactaga sibirica), Midday jird (Meriones meridianus), and Desert hamster (Phodopus roborovskii). Genomic analyses unveil that desert adaptation of the four species mainly result from similar metabolic pathways, such as arachidonic acid metabolism, thermogenesis, oxidative phosphorylation, insulin related pathway, DNA repair and protein synthesis and degradation. However, the specific evolved genes in the same adaptative molecular pathway often differ in the four species. We also reveal similar niche selection but different demographic histories and sensitivity to climate changes, which may be related to the diversified genomic adaptative features. In addition, our study suggests that nocturnal rodents have evolved some specific adaptative mechanism to desert environments compared to large desert animals. Our genomic resources will provide an important foundation for further research on desert genetic adaptations.
Partition decoupling model and method in power distribution network, part I: Optimised network partition model and process
Authors: Sheng Wanxing 
Published: January 2023

AbstractIn order to deal with the problems of complex optimisation model and computing speed in the multi‐objective operation control of the power distribution network, this paper is oriented to the basic model and process of the feeder partition decoupling method for the distribution network. Firstly, the necessity of developing partition decoupling for the distribution network is expounded according to the development status and control mode of the complex distribution network. Secondly, the objective models commonly used in the distribution network optimisation control are given to illustrate the importance of partition decoupling for the complex distribution network, including line loss and voltage offset. Finally, three general decoupling equivalent models are presented, namely Ward equivalent model, virtual generator equivalent model, and radial equivalent independent model, and then the partition decoupling equivalent process is proposed.