bluuebunny/crossref_metadata_2025_split huggingface dataset:
Can home care work be organized to promote health among the workers while maintaining productivity? An investigation into stakeholders’ perspectives on organizational work redesign concepts based on the Goldilocks Work principles
Authors: Liaset Ingeborg Frostad , Fimland Marius Steiro , Holtermann Andreas , Mathiassen Svend Erik , Redzovic Skender 
Published: June 2023

AbstractBackgroundDue to the aging population, the need for home care services is increasing in most Western countries, including Norway. However, the highly physical nature of this job could contribute to make recruiting and retaining qualified home care workers (HCWs) challenging. This issue may be overcome by adopting the Goldilocks Work principles, aiming at promoting workers’ physical health by determining a “just right” balance between work demands and recovery periods while maintaining productivity. The aim of this study was to 1) gather suggestions from home care employees on suitable organizational (re)design concepts for promoting HCWs’ physical health and 2) have researchers and managers define actionable behavioral aims for the HCWs for each proposed (re)design concept and evaluate them in the context of the Goldilocks Work principles.MethodsHCWs, safety representatives, and operation coordinators (n = 14) from three Norwegian home care units participated in digital workshops led by a researcher. They suggested, ranked, and discussed redesign concepts aimed at promoting HCWs’ health. The redesign concepts were subsequently operationalized and evaluated by three researchers and three home care managers.ResultsWorkshop participants suggested five redesign concepts, namely "operation coordinators should distribute work lists with different occupational physical activity demands more evenly between HCWs", "operation coordinators should distribute transportation modes more evenly between HCWs", "Managers should facilitate correct use of ergonomic aids and techniques", "HCWs should use the stairs instead of the elevator", and "HCWs should participate in home-based exercise training with clients". Only the first two redesign concepts were considered to be aligned with the Goldilocks Work principles. A corresponding behavioral aim for a “just right” workload was defined: reduce inter-individual differences in occupational physical activity throughout a work week.ConclusionsOperation coordinators could have a key role in health-promoting organizational work redesign based on the Goldilocks Work principles in home care. By reducing the inter-individual differences in occupational physical activity throughout a work week, HCWs’ health may be improved, thus reducing absenteeism and increasing the sustainability of home care services. The two suggested redesign concepts should be considered areas for evaluation and adoption in practice by researchers and home care services in similar settings.
Generation, evolution, interfering factors, applications, and challenges of patient-derived xenograft models in immunodeficient mice
Authors: Zeng Mingtang , Ruan Zijing , Tang Jiaxi , Liu Maozhu , Hu Chengji , Fan Ping , Dai Xinhua 
Published: June 2023

AbstractEstablishing appropriate preclinical models is essential for cancer research. Evidence suggests that cancer is a highly heterogeneous disease. This follows the growing use of cancer models in cancer research to avoid these differences between xenograft tumor models and patient tumors. In recent years, a patient-derived xenograft (PDX) tumor model has been actively generated and applied, which preserves both cell–cell interactions and the microenvironment of tumors by directly transplanting cancer tissue from tumors into immunodeficient mice. In addition to this, the advent of alternative hosts, such as zebrafish hosts, or in vitro models (organoids and microfluidics), has also facilitated the advancement of cancer research. However, they still have a long way to go before they become reliable models. The development of immunodeficient mice has enabled PDX to become more mature and radiate new vitality. As one of the most reliable and standard preclinical models, the PDX model in immunodeficient mice (PDX-IM) exerts important effects in drug screening, biomarker development, personalized medicine, co-clinical trials, and immunotherapy. Here, we focus on the development procedures and application of PDX-IM in detail, summarize the implications that the evolution of immunodeficient mice has brought to PDX-IM, and cover the key issues in developing PDX-IM in preclinical studies.
Nanostructures for prevention, diagnosis, and treatment of viral respiratory infections: from influenza virus to SARS-CoV-2 variants
Authors: Sharifi Esmaeel , Yousefiasl Satar , Trovato Maria , Sartorius Rossella , Esmaeili Yasaman , Goodarzi Hamid , Ghomi Matineh , Bigham Ashkan , Moghaddam Farnaz Dabbagh , Heidarifard Maryam , Pourmotabed Samiramis , Nazarzadeh Zare Ehsan , Paiva-Santos Ana Cláudia , Rabiee Navid , Wang Xiangdong , Tay Franklin R. 
Published: June 2023

AbstractViruses are a major cause of mortality and socio-economic downfall despite the plethora of biopharmaceuticals designed for their eradication. Conventional antiviral therapies are often ineffective. Live-attenuated vaccines can pose a safety risk due to the possibility of pathogen reversion, whereas inactivated viral vaccines and subunit vaccines do not generate robust and sustained immune responses. Recent studies have demonstrated the potential of strategies that combine nanotechnology concepts with the diagnosis, prevention, and treatment of viral infectious diseases. The present review provides a comprehensive introduction to the different strains of viruses involved in respiratory diseases and presents an overview of recent advances in the diagnosis and treatment of viral infections based on nanotechnology concepts and applications. Discussions in diagnostic/therapeutic nanotechnology-based approaches will be focused on H1N1 influenza, respiratory syncytial virus, human parainfluenza virus type 3 infections, as well as COVID-19 infections caused by the SARS-CoV-2 virus Delta variant and new emerging Omicron variant.Graphical Abstract
HIF1α-dependent hypoxia response in myeloid cells requires IRE1α
Authors: Mawambo Gaëlle , Oubaha Malika , Ichiyama Yusuke , Blot Guillaume , Crespo-Garcia Sergio , Dejda Agnieszka , Binet François , Diaz-Marin Roberto , Sawchyn Christina , Sergeev Mikhail , Juneau Rachel , Kaufman Randal J. , Affar El Bachir , Mallette Frédérick A. , Wilson Ariel M. , Sapieha Przemyslaw 
Published: June 2023

AbstractCellular adaptation to low oxygen tension triggers primitive pathways that ensure proper cell function. Conditions of hypoxia and low glucose are characteristic of injured tissues and hence successive waves of inflammatory cells must be suited to function under low oxygen tension and metabolic stress. While Hypoxia-Inducible Factor (HIF)-1α has been shown to be essential for the inflammatory response of myeloid cells by regulating the metabolic switch to glycolysis, less is known about how HIF1α is triggered in inflammation. Here, we demonstrate that cells of the innate immune system require activity of the inositol-requiring enzyme 1α (IRE1α/XBP1) axis in order to initiate HIF1α-dependent production of cytokines such as IL1β, IL6 and VEGF-A. Knockout of either HIF1α or IRE1α in myeloid cells ameliorates vascular phenotypes in a model of retinal pathological angiogenesis driven by sterile inflammation. Thus, pathways associated with ER stress, in partnership with HIF1α, may co-regulate immune adaptation to low oxygen.
MiR-137 promotes TLR4/NF-κB pathway activity through targeting KDM4A, inhibits osteogenic differentiation of human bone marrow mesenchymal stem cells and aggravates osteoporosis
Authors: Yu Ying-feng , Yao Pei-quan , Wang Zhi-kun , Xie Wen-wei 
Published: June 2023

Abstract
Purpose
As the global population ages rapidly, osteoporotic fractures have become an important public health problem. Previous studies have suggested that miR-137 is involved in the regulation of bone formation, but its specific regulatory mechanism remains unclear. In this study, we aimed to explore the expression, role, and regulatory mechanism of miR-137 in the osteogenic differentiation of human bone marrow mesenchymal stem cells (hBMSCs).

Methods
hBMSCs were induced into osteoblasts at first, and the expression level of miR-137 at different time points was detected. After knockdown and overexpression of miR-137, the effect of miR-137 on the osteogenic differentiation of hBMSCs was examined through alkaline phosphatase (ALP) staining and Alizarin Red staining. Western blotting was performed to detect the expression of runt-related transcription factor 2 (Runx2), osteocalcin (OCN), and toll-like receptor 4 (TLR4)/nuclear factor-κB (NF-κB) pathway. Bioinformatics websites were used to predict the target binding sites for miR-137 and KDM4A, and the results were validated using luciferase reporter gene experiments. Moreover, the ALP activity, calcium nodule formation, and activation of Runx2, OCN, and TLR4/NF-κB pathways were observed after knockdown of KDM4A.

Results
The expression of miR-137 decreased during osteogenic differentiation. Knockdown of miR-137 expression increased the osteogenic ability of hBMSCs, while overexpression of it weakened the ability. Through the activation of the TLR4/NF-κB pathway, miR-137 inhibited osteogenic differentiation. KDM4A was identified as a predicted target gene of miR-137. After knocking down KDM4A expression, the osteogenic ability of hBMSCs was diminished, and the TLR4/NF-κB pathway was activated. Furthermore, the osteogenic ability of hBMSCs was partially restored and the activation level of TLR4/NF-κB was reduced after miR-137 knockdown.

Conclusion
MiR-137 enhances the activity of the TLR4/NF-κB pathway by targeting KDM4A, thereby inhibiting the osteogenic differentiation of hBMSCs and exacerbating osteoporosis.
Comparison of clinical outcome of lumbar spinal stenosis surgery in patients with and without osteoporosis: a prospective cohort study
Authors: Aghajanloo Mashhood , Abdoli Ali , Poorolajal Jalal , Abdolmaleki Sajjad 
Published: June 2023

Abstract
Background
Osteoporosis is one of the most important risk factors for failure of the spine instrumentation. Management of patients with osteoporosis who requires spinal surgery because of the difficulty in instrument placement and the potential complications is still a challenge. This study was designed to evaluate the clinical outcome of lumbar spinal canal stenosis after instrumentation in patients with and without osteoporosis.

Methods
This prospective cohort study was performed from June 2018 to December 2020, in Be'sat Hospital, Hamadan, Iran. The sample consisted of patients over 50 years old referred to Be'sat Hospital with a diagnosis of lumbar spinal canal stenosis who underwent instrumental surgery (n = 107). Based on bone densitometry, the sample was divided into two groups with osteoporosis (n = 34) and without osteoporosis (n = 73). To collect data, we used a three-part researcher-made questionnaire (demographic information, medical records information, and paraclinical parameters). Statistical analyzes were performed by the Fisher Exact, chi-square, independent t-test, Multiple ANCOVA, Mann–Whitney and the Rank Wilcoxson tests using Stata version 17 software.

Results
The mean age (SD) of patients in the two groups with and without osteoporosis was 67.9 (7.0) and 59.1 (5.1) years, respectively (p = 0.001). The results indicated that a significant difference was observed between the two groups in sex (p = 0.032), educational status (p = 0.001), marital status (p = 0.023), employment status (p = 0.004), menopausal status (p = 0.018), taking corticosteroids (p = 0.028), and body mass index (p = 0.015). Also, there was a significant difference between two groups in the loosening of instrument (p = 0.039), the postoperative pain intensity (p = 0.007), fusion (p = 0.047), and neurogenic claudication (p = 0.003). Based on multiple ANCOVA test, there was not a significant difference between two groups in the clinical and paraclinical charatecristics (p > 0.05). The mean (SD) of T-Score in the osteoporosis group was 3.06 (0.37).

Conclusion
This study provides evidence that there is no significant difference in the clinical outcomes of lumbar spine instrumentation due to spinal canal stenosis in patients with and without osteoporosis. Because of the high cost of specific instrumentation developed for patients with osteoporosis and their unavailability, it seems that the use of conventional instrumentation along with complete treatment of osteoporosis can help improve the clinical outcome of surgery in these patients.
BCAT1 controls embryonic neural stem cells proliferation and differentiation in the upper layer neurons
Authors: Zhang Shukui , Zhao Jinyue , Zhao Cheng , Su Libo , Jiao Jianwei 
Published: June 2023

AbstractThe regulation of neural stem cell (NSC) proliferation and differentiation during brain development is a precisely controlled process, with the production of different neuronal subtypes governed by strict timelines. Glutamate is predominantly used as a neurotransmitter by the subtypes of neurons in the various layers of the cerebral cortex. The expression pattern of BCAT1, a gene involved in glutamate metabolism, in the different layers of neurons has yet to be fully understood. Using single-cell data, we have identified seven different states of NSCs and found that state 4 is closely associated with the development of projection neurons. By inferring the developmental trajectory of different neuronal subtypes from NSC subsets of this state, we discovered that BCAT1 is involved in the regulation of NSC proliferation and differentiation and is specifically highly expressed in layer II/III and IV neurons. Suppression of BCAT1 through shRNA resulted in a reduction in NSC proliferation and an abnormal development of layer II/III and IV neurons. These findings provide new insights into the role of BCAT1 in the regulation of NSC behavior and neuronal development.
The mediating role of inflammation in the association between pregnancy loss history and gestational diabetes mellitus
Authors: Li Qiong , Wang Haixia , Sun Lijun , Wang Peng , Yin Wanjun , Ma Shuangshuang , Tao Ruixue , Ge Jinfang , Zhu Peng 
Published: June 2023

Abstract
Background
To assess the association of pregnancy loss history with an elevated risk of Gestational diabetes mellitus (GDM) and to investigate whether this association was mediated by high-sensitivity C-reactive protein (hs-CRP).

Methods
We prospectively collected venous blood and pregnancy loss history information from 4873 pregnant women at 16–23 weeks of gestation from March 2018 to April 2022. Hs-CRP concentrations were measured from collected blood samples. A 75 g fasting glucose test was performed at 24 to 28 weeks of gestation for the diagnosis of GDM, with data obtained from medical records. Multivariate linear or logistic regression models and mediation analysis were used to examine the relationships between pregnancy loss history, hs-CRP, and GDM.

Results
A multivariable-adjusted logistic regression analysis revealed that compared with pregnant women with no induced abortion history, subjects with 1 and ≥ 2 induced abortions had a higher risk for GDM (RR = 1.47, 95% CI = 1.19–1.81; RR = 1.63, 95% CI = 1.28–2.09). Additionally, the mediation analysis indicated this association was mediated by an increased hs-CRP level with a 20.4% of indirect effect ratio. However, no significant association between a history of miscarriage and the prevalence of GDM was observed.

Conclusions
A history of induced abortion was significantly associated with an increased risk of GDM, and this association occurred in a dose-response effect. Hs-CRP may be accounted for a mediation effect in the pathways linking induced abortion history with GDM.
Association between neck circumference and diabetes mellitus: a systematic review and meta-analysis
Authors: Li Dandan , Zhao Yuxin , Zhang Lifang , You Qiqi , Jiang Qingqing , Yin Xiaoxv , Cao Shiyi 
Published: June 2023

Abstract
Background
Despite that several original researchers have investigated the association between neck circumference (NC) and the risk of diabetes mellitus (DM), their results remain controversial. This review aimed to quantitatively determine the risk of DM in relation to the NC.

Methods
We conducted a literature search of PubMed, Embase, and the Web of Science from these databases’ inception through September 2022 to identify observational studies that examined the association between NC and the risk of DM. A meta-analysis of the random-effects model was applied to combine the results of the enrolled studies.

Results
Sixteen observational studies involving 4,764 patients with DM and 26,159 participants were assessed. The pooled results revealed that NC was significantly associated with the risk of type 2 DM (T2DM) (OR = 2.17; 95% CI: 1.30–3.62) and gestational DM (GDM) (OR = 1.31; 95% CI: 1.17–1.48). Subgroup analysis revealed that after controlling for BMI, the relationship between the NC and T2DM remained statistically significant (OR = 1.94; 95% CI: 1.35–2.79). Moreover, the pooled OR of T2DM was found to be 1.16 (95% CI: 1.07–1.27) for an increment per each centimeter in the NC.

Conclusions
Integrated epidemiological evidence supports the hypothesis that a greater NC is associated with an increased risk of T2DM and GDM.
A new dosing regimen of ropeginterferon alfa-2b is highly effective and tolerable: findings from a phase 2 study in Chinese patients with polycythemia vera
Authors: Jin Jie , Zhang Lei , Qin Albert , Wu Daoxiang , Shao Zonghong , Bai Jie , Chen Suning , Duan Minghui , Zhou Hu , Xu Na , Zhang Sujiang , Zuo Xuelan , Du Xin , Wang Li , Li Pei , Zhang Xuhan , Li Yaning , Zhang Jingjing , Wang Wei , Shen Weihong , Zagrijtschuk Oleh , Urbanski Raymond , Sato Toshiaki , Xiao Zhijian 
Published: June 2023

AbstractRopeginterferon alfa-2b represents a new-generation pegylated interferon-based therapy and is administered every 2–4 weeks. It is approved for polycythemia vera (PV) treatment in the United States and Europe with a starting dose of 100 µg (50 µg for patients receiving hydoxyurea) and intra-patient dose titrations up to 500 µg at 50 µg increments, which took approximately 20 or more weeks to reach a plateau dose level. This study aimed to assess ropeginterferon alfa-2b at an alternative dosing regimen with a higher starting dose and quicker intra-patient dose titrations, i.e., the 250–350–500 μg schema, in 49 Chinese patients with PV with resistance or intolerance to hydroxyurea. The primary endpoint of the complete hematologic response rate at treatment weak 24 was 61.2%, which was notably higher than 43.1% at 12 months with the approved dosing schema. The JAK2V617F allele burden decreased from baseline to week 24 (17.8% ± 18.0%), with one patient achieving a complete molecular response. Ropeginterferon alfa-2b was well-tolerated and most adverse events (AEs) were mild or moderate. Common AEs included alanine aminotransferase and aspartate aminotransferase increases mostly at grade 1 or 2 levels. Patients did not present with jaundice or significant bilirubin level increase. No grade 4 or 5 AEs occurred. Seven patients (14.3%) experienced reversible, drug-related grade 3 AEs. No AEs led to treatment discontinuation. Ropeginterferon alfa-2b at the 250–350–500 μg regimen is highly effective and well-tolerated and can help patients achieve greater and rapid complete hematologic and molecular responses.Clinical Trial Registration: This trial is registered at ClinicalTrials.gov (Identifier: NCT05485948) and in China (China National Medical Products Administration Registration Number: CTR20211664).
The role of 18F-FDG PET/CT in distinguishing benign from malignant portal vein thrombosis
Authors: Abokoura Sameh , Ellaban Heba Said , Aly Rasha Abdelhafiz 
Published: June 2023

Abstract
Background
Diagnosis of tumour thrombosis and differentiating it from benign thrombosis are essential for managing patients, planning treatments, and minimising unneeded anticoagulation therapy. Bland thrombi occur in both cancer and non-cancer patients; tumour thrombi and bland can coexist. 18F-fluorodeoxyglucose positron emission tomography/computed tomography (18F-FDG PET/CT) is useful in detecting and diagnosing tumour thrombosis and distinguishing it from benign thrombosis.

Objective
This study’s aim was to assess the value of 18F-FDG PET/CT in distinguishing benign from malignant portal vein thrombosis (PVT) in liver cirrhosis patients.

Methods
A retrospective study was conducted on 38 patients who had PVT that was histopathologically confirmed and performed 18F-FDG PET/CT scans at our institute between January 2021 and April 2022. For all patients, sociodemographic data, visual analysis, semiqualitative analysis (SUVmax value), and associated hepatic pathology were collected.

Results
The SUVmax values were significantly higher in the tumour thrombosis group (6.26 ± 1.94), compared to the bland thrombosis group (1.79 ± 0.69), (P < 0.001). The ROC curve of semiqualitative analysis (SUVmax) revealed a sensitivity of 96.3% and a specificity of 36.4%, at area under curve of 0.827 with SUVmax > 3.5 as the pathological cut-off value to distinguish tumour from bland thrombi.

Conclusions
By using semiqualitative analysis, 18F-FDG PET/CT is a valuable new technique in differentiating between neoplastic and bland PV thrombi, with optimal cut-off SUVmax value > 3.5 as a criterion.
GC-MS analysis of honeybee products derived from medicinal plants
Authors: Montaser Maha , Sayed Ahmed M. , Bishr Mokhtar M. , Zidan Ehab W. , Zaki Mohamed A. , Hassan Hossam M. , Mohammed Rabab , Hifnawy Mohamed S. 
Published: June 2023

Abstract
Background
Honeybees provide a wealth of valuable natural products containing health-promoting bioactive compounds, including honey, bee bread, bee venom, bee pollen, propolis, and royal jelly. In the present study, we investigated the chemical composition of four honeybee products (bees, honey, royal jelly, and bee bread) derived from three medicinal plants (marjoram, trifolium, and citrus) using headspace GC-MS.

Results
GC-MS analysis coupled with the headspace method resulted in identification of 24 volatile compounds in marjoram honey, 14 volatile compounds in trifolium honey, and 25 volatile compounds in citrus honey, e.g., some of these compounds appeared in all three types of honey aroma, which are 2,3-dihydro-3,5-dihydroxy-6-methyl-4H-pyran-4-one, 2-furancarboxaldehyde, 5-(hydroxymethyl) and other unique compounds specific for each type where 23 compounds were from marjoram bees, 38 compounds from trifolium bees, and about 37 compounds were identified in citrus bees where 2,4-decadienal, (E, E) and methyl N-methyl anthranilate were common in all. Furthermore, the volatile compounds of all three types of royal jelly aroma were acetic acid, 2,3-dihydro-3,5-dihydroxy-6-methyl-4H-pyran-4-one, 8-nonen-2-one and furfural where one compound appeared in both marjoram and trifolium royal jelly that is 2-furancarboxaldehyde,5-(Hydroxymethyl) and the volatile compounds in marjoram and citrus are 2,3-butanediol and 5-methylfurfural also only one volatile compound appear in both trifolium and citrus royal jelly that is furfur alcohol, Finally, 3 compounds from marjoram bee bread, 30 volatile compounds from trifolium bee bread, and 3 volatile compounds in citrus bee bread were identified.

Conclusions
A detailed metabolomic analysis of the four honey product groups revealed an intriguing chemical diversity, with each sample exhibiting its own chemical fingerprint.