bluuebunny/crossref_metadata_2025_split huggingface dataset:
Biosensor-integrated transposon mutagenesis reveals rv0158 as a coordinator of redox homeostasis in Mycobacterium tuberculosis
Authors: Singh Amit , Shee Somnath , TV Reshma , Mohanraj Karthikeyan , Das Mayashree , Malhotra Nitish , Bandopadhyay Devleena , Beig Hussain , Birua Shalini , Niphadkar Shreyas , Nagarajan Sathyanarayanan , Sinha Vikrant Kumar , Thakur Chandrani , Rajmani Raju , Chandra Nagasuma , Laxman Sunil , Singh Mahavir , Samal Areejit , Seshasayee Aswin Sai Narain 
Published: July 2023

Abstract
Mycobacterium tuberculosis (Mtb) is evolutionarily equipped to resist exogenous reactive oxygen species but shows vulnerability to an increase in endogenous ROS (eROS). Since eROS is an unavoidable consequence of aerobic metabolism, understanding how Mtb manages eROS levels is essential yet needs to be characterized. By combining the Mrx1-roGFP2 redox biosensor with transposon mutagenesis, we identified 368 genes (redoxosome) responsible for maintaining homeostatic levels of eROS in Mtb. Integrating redoxosome with a global network of transcriptional regulators revealed a hypothetical protein (Rv0158) as a critical node managing eROS in Mtb. Disruption of rv0158 (rv0158 KO) impaired growth, redox balance, respiration, and metabolism of Mtb on glucose but not on fatty acids. Importantly, rv0158 KO exhibited enhanced growth on propionate, and the Rv0158 protein directly binds to methylmalonyl-CoA, a key intermediate in propionate catabolism. Metabolite profiling, ChIP-Seq, and gene-expression analyses indicate that Rv0158 coordinates metabolic neutralization of propionate toxicity by regulating the methylcitrate cycle. Disruption of rv0158 enhanced the sensitivity of Mtb to oxidative stress, nitric oxide, and anti-TB drugs. Lastly, rv0158 KO showed poor survival in macrophages and persistence defect in mice. Our results suggest that Rv0158 is a metabolic integrator for carbon metabolism and redox balance in Mtb.
Persistent organic pollutants profile of sediments from marine protected areas: the Northern Persian Gulf
Authors: Ghaemi Maryam , Soleimani Farshid , Gholamipour Sara 
Published: July 2023

Abstract
Marine protected areas (MPAs) are one of the policy tools to support marine biodiversity conservation and sustainable use of marine resources. The distribution, sources, and ecological risk assessment of persistent organic pollutants (POPs), including polycyclic aromatic hydrocarbon (PAHs), total petroleum hydrocarbons (TPHs), polychlorinated biphenyls (PCBs), trace metals, and metalloids in sediments of MPAs in the northern Persian Gulf were evaluated for the first time in this study. The Σ16PAHs ranged from 4.65 to 20.86 µg/kg dw. The molecular ratios and ring's pattern of PAHs suggested a mixed origin with a predominance of pyrogenic sources. The TPHs concentration varied from 5.21 to 17.90 µg/g dw. Ecological risk assessment suggested that sediment samples in Bushehr Province’s MPAs can be categorized as medium risk. The mean concentration of ∑18PCB was 0.345–0.419 ng/g dw, and the main components correspond to PCB-77, PCB-105, PCB-81, PCB-101, and PCB-114. The mean concentration of As, Co, Cr, Ni, V, Mg, Pb, Zn, Cu, Al, and Fe varied from 4.79–9.69, 2–12, 39–142, 18–90, 15–58, 184–425, 7-459, 6-424, 4–20 µg/g dw, 0.75–4.12%, and 0.35–1.62%, respectively. The results of this study provided the background information on the extent of POPs contamination in the sediment and highlighted the need to further control pollution in MPAs.
Linking cerebral hemodynamics and ocular microgravity-induced alterations through an in silico-in vivo head-down tilt framework
Authors: Fois Matteo , Diaz-Artiles Ana , Zaman Syeda Yasmin , Ridolfi Luca , Scarsoglio Stefania 
Published: July 2023

Abstract
Head-down tilt (HDT) has been widely proposed as terrestrial analogue of microgravity and used also to investigate the occurrence of the spaceflight associated neuro-ocular syndrome (SANS), which is currently considered one of the major health risks for human spaceflight. We propose here a novel, in vivo validated numerical framework to simulate the acute ocular-cerebrovascular response to 6° HDT and to explore the etiology and pathophysiology of SANS.
The model links cerebral and ocular posture-induced hemodynamics, simulating the response of the main cerebrovascular mechanisms, as well as the relationship between intracranial and intraocular pressure to HDT. Our results from short-term (10 min) 6° HDT show increased hemodynamic pulsatility in the proximal-to-distal/capillary-venous cerebral direction, a marked decrease (-43%) in ocular translaminar pressure, and an increase (+31%) in ocular perfusion pressure, suggesting a plausible explanation of the underlying mechanisms at the onset of ocular globe deformation and edema formation over longer time scales.
Association Between Needle Track Bleeding and Postoperative Immediate Pneumothorax in CT-Guided Percutaneous Transthoracic Lung Biopsies: A Cross-Sectional Study
Authors: Zhou Shao-Quan , Luo Fang , Li Kang , Ran Xiong , Lv Fu-Rong 
Published: July 2023

Abstract
Background:
The relationship between Needle Track Bleeding (NTB) and the occurrence of postoperative immediate pneumothorax remains unclear.
Methods:
In our cross-sectional study, we conducted a retrospective collected of data from 674 consecutive patients who underwent CT-guided percutaneous transthoracic lung biopsies between 2019 and 2022. A logistic regression model was employed to explore the association between NTB and postoperative immediate pneumothorax, and restricted cubic spline curves was used to investigate the link and its explicit curve shape. A sensitivity analysis was performed by transforming the continuous NTB into categorical variable and calculated an E-value.
Results:
A total of 453 participants (47.90% male) were included in our analysis. The postoperative immediate pneumothorax rate was 41.05% (186/453). We found a negative correlation between NTB and postoperative immediate pneumothorax (OR = 0.91, 95%CI: 0.88–0.95) after adjusting for confounding factors. This relationship was nonlinear, with a key inflection point at NTB of 8mm. No significant link was noted for NTB > 8mm (OR = 0.98, 95%CI: 0.95–1.02), while a protective association was observed for NTB ≤ 8mm (OR = 0.74, 95%CI: 0.66–0.81).
Conclusion:
NTB showed a nonlinear, protective correlation with postoperative immediate pneumothorax. However, when NTB exceeded 8mm, the protective association was not observed.
The impact of histologic subtype on primary site surgery in the management of metastatic lobular versus ductal breast cancer: a population based study from the National Cancer Database
Authors: Rothschild Harriet T , Clelland Elle N , Abel Mary Kathryn , Chien A Jo , Shui Amy M , Esserman Laura , Khan Seema A , Mukhtar Rita A 
Published: July 2023

Abstract
Purpose
Primary site surgery for metastatic breast cancer improves local control but does not impact overall survival. Whether histologic subtype influences patient selection for surgery is unknown. Given differences in surgical management between early-stage lobular versus ductal disease, we evaluated the impact of histology on primary site surgery in patients with metastatic breast cancer.
Methods
The National Cancer Database (NCDB, 2010–2016) was queried for patients with stage IV HR-positive, HER2-negative invasive lobular carcinoma (ILC) and invasive ductal carcinoma (IDC). We compared clinicopathologic features, primary site surgery rates, and outcomes by histologic subtype. Multivariable Cox proportional hazard models with and without propensity score matching were used for overall survival (OS) analyses.
Results
In 25,294 patients, primary site surgery was slightly but significantly less common in the 6,123 patients with ILC compared to the 19,171 patients with IDC (26.9% versus 28.8%, p = 0.004). Those with ILC were less likely to receive chemotherapy (41.3% versus 47.4%, p < 0.0001) or radiotherapy (29.1% versus 37.9%, p < 0.0001), and had shorter OS. While mastectomy rates were similar, those with ILC had more positive margins (10.6% versus 8.3%, p = 0.005). In both groups, the odds of undergoing surgery decreased over time, and were higher in younger patients with T2/T3 tumors and higher nodal burden.
Conclusion
Lobular histology is associated with less primary site surgery, higher positive margin rates, less radiotherapy and chemotherapy, and shorter OS compared to those with HR-positive HER2-negative IDC. These findings support the need for ILC-specific data and treatment approaches in the setting of metastatic disease.
Common causes of maternal mortality based on ICD-MM: A systematic review and meta- analysis
Authors: Alipour Jahanpour , Payandeh Abolfazl , Karimi Afsaneh 
Published: July 2023

Abstract
Background
Maternal mortality is a universal public health challenge. The present study was conducted to estimate the global prevalence of maternal death causes through a systematic review and meta-analysis.
Methods
A systematic literature search was conducted using various databases, including Web of Science, PubMed, Scopus, ScienceDirect, Cochrane Library, as well as Persian databases such as MagIran and SID. The search encompassed articles published until August 21, 2022. Thirty-four eligible articles were included in the final analysis. Analysis was performed using a meta-analysis approach. The exact Clopper-Pearson confidence intervals, heterogeneity assessment, and random effects models with Mantel-Haenszel methods were employed using the STATA software version 14.2.
Results
The most prevalent causes of maternal deaths, listed in descending order from highest to lowest prevalence, were non-obstetric complications (48.32%), obstetric hemorrhage (17.63%), hypertensive disorders of pregnancy, childbirth, and the puerperium (14.01%), other obstetric complications (7.11%), pregnancy with abortive outcome (5.41%), pregnancy-related infection (5.26%), unanticipated complications of management (2.25%), unknown/undetermined causes (2.01%), and coincidental causes (1.59%), respectively.
Conclusion
Accurate and timely diagnosis and treatment of conditions complicating pregnancy, childbirth, and the puerperium continue to pose a significant gap in high-quality care for pregnant women worldwide, particularly in developing countries where the incidence is higher. To reduce the burden of maternal mortality causes, it is crucial to implement effective screening mechanisms for high-risk mothers during pregnancy, childbirth, and the puerperium. Additionally, increasing awareness and promoting self-care management among women of reproductive age can play a significant role. Our findings offer comprehensive and standardized information on the prevalence of maternal mortality causes using the ICD-MM classification. This information can be utilized by policymakers and managers at various levels to facilitate necessary planning aimed at reducing the burden of maternal mortality causes.
Solenoid magnetic field simulation capability analysis based on current inversion
Authors: Lu Binjie , Zhang Xiaobin , Dai Zhonghua 
Published: July 2023

Abstract
In order to analyze the simulation capability of the solenoid to the ship's magnetic field, an equivalent magnetic dipole array model is used. Calculate the solenoid magnetic field. Based on the empirical setting of solenoid configuration parameters, combined with the measured data of the ship model magnetic field, the least squares method was used to invert the solenoid current based on the plane magnetic field vector inversion solenoid constant current, the planar magnetic field scalar inversion solenoid constant current, the planar magnetic field vector inversion solenoid time-varying current, and the planar magnetic field scalar inversion solenoid time-varying current and other four methods. The relative error of the solenoid magnetic field vector and scalar and the ship model magnetic field vector and scalar generated by the four inversion methods is calculated. The simulation capabilities of different inversion methods are compared and analyzed, and finally the current inversion method of solenoid simulating the ship's magnetic field is given.
Reading Diabetes Prevalence in India: Awareness vs. Affluence
Authors: Jena Abhinesh , Mishra Udaya Shankar , - Rinju 
Published: July 2023

Abstract

The literature is convincing on increasing affluence being a driver of rising prevalence of diabetes that is verified here in terms of associating an additional dimension of awareness. Given the doubling of prevalence of diabetes in India in a span of 5 years based on a comparison between NFHS-4 and NFHS-5, this is an attempt towards dis-entangling bearing of affluence/awareness on the rising trend of diabetes. Here we compute the characteristics odds and its conditional equivalent to understand the influence of the twin axis of awareness and affluence represented by education and wealth quintile. The awareness level odds of experiencing diabetes have widened more than the same owing to affluence as indicated by the gap in odds of the two across categories over time. Overall findings suggest despite the systematic role of affluence in shaping the disease prevalence, rising awareness serves as a key to its compression.
Positive and negative plant-plant interactions influence seedling establishment at both high and low elevations
Authors: Hischier Chantal M. , Lambers Janneke Hille Ris , Iseli Evelin , Alexander Jake M. 
Published: July 2023

Abstract
According to the stress gradient hypothesis (SGH), plant-plant interactions are expected to shift from predominantly negative (i.e. competition) to predominantly positive (i.e. facilitation) along gradients of environmental severity. The SGH has been particularly useful as a framework for understanding how plants interact with each other across elevation gradients, partly as a proxy for future responses to climate change. However, most experiments examine the net effects of interactions by growing plants in either the presence or absence of neighbours, thereby neglecting the interplay of both negative and positive effects acting simultaneously within communities. To partially unravel these effects, we tested how the seedling establishment of 10 montane grassland plants varied in the presence versus absence of plant communities at two sites along an elevation gradient. We created a third experimental treatment that retained the main hypothesised benefits of plant neighbours (microsite amelioration), while reducing a key negative effect (competition for soil resources). In contrast to predictions of the SGH, we found evidence for net positive effects of vegetation at the low site, and net negative effects at the high site. Interestingly, the surprisingly negative effects of plant neighbours at high elevation were driven by high establishment rates of low elevation grasses in bare soil plots. At both sites, establishment rates were highest in artificial vegetation (after excluding two low elevation grasses at the high site), indicating that positive effects of above-ground vegetation are partially offset by their negative effects. Our results demonstrate that both competition and facilitation act jointly to affect community structure across environmental gradients, while challenging the view that competition is weak at high elevation in temperate mountain regions. Consequently, plant-plant interactions are likely to influence the establishment of new, and persistence of resident, species in mountain plant communities as environments change.
Identification and characterization of CSE family members in cucumber reveals CsCSE5, a positive regulator of pathogen resistance
Authors: Yu Yong bo , He Jia jing , Liu Ling hao , Zhao Hong yan , Zhang Meng meng , Hong Jing hang , Meng Xiang nan , Fan Hai yan 
Published: July 2023

Abstract
Caffeoyl shikimate esterase (CSE), a key enzyme in the lignin biosynthetic pathway, has recently been characterized to play a key role in defense against pathogenic infection in plants. However, a systematic analysis of the CSE gene family in cucumber (Cucumis sativus) has not yet been conducted. Here, we identified eight CsCSEgenes from the cucumber genome via bioinformatic analyses, and these genes were unevenly distributed on chromosomes 1, 3, 4, and 5. Results from multiple sequence alignment indicated that the CsCSE proteins had CSE activity. Phylogenetic analysis of gene structure and protein motifs revealed the conservation and diversity of the CsCSE gene family. Collinearity analysis showed that CsCSEgenes had high homology with CSE genes in Benincasa hispida. Cis-acting element analysis of the promoters suggested that CsCSE genes might play important roles in growth, development, and stress tolerance. Expression pattern analysis indicated that CsCSE5 might be involved in regulating the resistance of cucumber to pathogens. Functional verification data confirmed that CsCSE5 positively regulates the resistance of cucumber to Podosphaera xanthii and Corynespora cassiicola. The results of our study provide information that will aid the genetic improvement of resistant cucumber varieties.
CD39 and LDHA affects the prognostic role of NLR in metastatic melanoma patients treated with immunotherapy
Authors: Mallardo Domenico , Fordellone Mario , White Andrew , Ottaviano Margaret , Sparano Francesca , Bailey Michael , Facchini Arianna , Ong Sufey , Maiolino Piera , Caracò Corrado , Church Sarah , Cavalcanti Ernesta , Warren Sarah , Budillon Alfredo , Cesano Alessandra , Simeone Ester , Chiodini Paolo , Ascierto Paolo A. 
Published: July 2023

Abstract
Background
Identifying response markers is highly needed to guide the treatment strategy in patients with metastatic melanoma.
Methods
A retrospective study was carried out in patients with unresectable/metastatic melanoma (stage IIIb–IV), treated with anti-PD-1 in the first line setting, to better explore the role and the timing of neutrophil/lymphocyte ratio (NLR) as potential biomarker of response. The relationship of NLR with inflammation-immune mediators and the underlying negative effect of raising NLR during immunotherapy, have been investigated with transcriptomic gene analysis.
Results
The results confirmed previous findings that a high baseline NLR is associated with a poorer prognosis and with higher serum level of lactate dehydrogenase (LDH), regardless of the presence of brain metastases. The transcriptomic analysis showed that high baseline NLR is associated with a characteristic gene signature CCNA1, LDHA and IL18R1, which is correlates with inflammation and tumorigenesis. Conversely, low baseline NLR is associated with the signature CD3, SH2D1A, ZAP70 and CD45RA, linked to the immune-activation. The genes positively associated with NLR (CD39 (ENTPD1), PTEN, MYD88, MMP9 and LDH) are involved in processes of immunosuppression, inflammation and tumor-promoting activity. Increased expression of CD39 correlated with TGFβ2, a marker of the N2 neutrophils with immunosuppressive activity.
Conclusions
These results suggest that increasing NLR is associated with an increased neutrophil population, with polarization to the N2 phenotype, and this process may be the basis for the negatively prognostic role of NLR.
Zeros of a One-parameter Family of Rational Harmonic Functions
Authors: Huang Yaru , Gao Junyang , Gao Linkui 
Published: July 2023

Abstract
This paper is devoted to investigating the number of zeros of a one-parameter familyof rational harmonic functions fc(z). It is considered to be as an analogue work on that of corresponding harmonic polynomials pc(z), which was investigated recently by Sandberg. Moreover, this paper explores the number of zeros of rational harmonic functions of rc(z). Our proofs rely on the Argument Principle for Harmonic Functions and the Poincare index.
Cutting tool wear prediction based on the multi-stage Wiener process
Authors: Wang Yuping , Chang Miaoxin , Huang Xianzhen , Li Yuxiong , Tang Jiwu 
Published: July 2023

Abstract
Cutting tools are one type of the critical components of the modern computer numerical control (CNC) machining system, whose failure can lead the whole system to fail or even cause substantive losses. Therefore, it is of great importance to study the method for tool wear prediction. A new model for wear prediction of cutting tools is established based on a multi-stage Wiener process, where the degradation rates of cutting tools are considered to change in three stages based on the typical cutting tool wear curve model. Firstly, the degradation processes of cutting tools are divided into three stages. Secondly, the parameter estimation for each stage of the degradation processes of cutting tools is completed respectively by utilizing the EM (expectation-maximization) algorithm. Then, the wear of cutting tools is predicted, and the reliability of cutting tools is analyzed by using a numerical integration simulation method based on the Monte Carlo algorithm. Finally, the proposed model is illustrated and verified via the flank wear data of cutting tools, and the prediction accuracy is measured by mean squared error (MSE) and the coefficient of determination (R^2). The prediction results show that the proposed model enables us to make more economical maintenance by delaying the tool replacement time with fewer degradation data. And compared to the traditional methods based on machine learning (ML), the proposed model can complete the wear prediction and reliability analysis more accurately.
Photospheric Velocities Measured at Mt. Wilson Show Zonal and Sectoral Flows Compose theTorsional Oscillations
Authors: Ulrich Roger K , Tran Tham , Boyden John 
Published: July 2023

Abstract
The methods for reducing the observations from the 150-foot Tower Telescope on Mt. Wilson are reviewed and a new method for determining the North/South (sectoral) and the East/West (zonal) velocity components is de-scribed and applied. Due to a calibration problem with the data prior to 1983, only observations between 1983 and 2013 are presented at this time. After sub-traction of latitude dependent averages over the 30-year period of observation the residual deviations in the sectoral and zonal flow velocities are well synchronized and correspond to what is widely recognized as the Torsional Oscillations. Both flow components need to be included in any model that replicates the Torsional Oscillations.
Dual antibody-aided mesoporous nanoreactor for H2O2 self-supplying chemodynamic therapy and checkpoint blockade immunotherapy in triple-negative breast cancer
Authors: Chen Ying-Tzu , Luo Ying-Xiang , Chan Shih-Hsuan , Chiu Wen-Yi , Yang Hung-Wei 
Published: July 2023

Abstract
Triple-negative breast cancer (TNBC) represents a formidable challenge due to the absence of estrogen receptor (ER), progesterone receptor (PR), and human epidermal growth factor receptor 2 (HER2) expression, rendering it unresponsive to conventional hormonal and targeted therapies. This study introduces the development of mesoporous nanoreactors (NRs), specifically mPDA@CuO2 NRs, as acid-triggered agents capable of self-supplying H2O2 for chemodynamic therapy (CDT). To enhance therapeutic efficacy, these NRs were further modified with immune checkpoint antagonists, specifically anti-PD-L1 and anti-CD24 antibodies, resulting in the formation of dual antibody-aided mesoporous nanoreactors (dAbPD−L1/CD24-mPDA@CuO2 NRs). These NRs were designed to combine CDT and checkpoint blockade immunotherapy (CBIT) for precise targeting of 4T1 TNBC cells. Remarkably, dAbPD−L1/CD24-mPDA@CuO2 NRs exhibited tumor-targeted CDT triggered by H2O2 and successfully activated immune cells including T cells and macrophages. This integrated approach led to a remarkable inhibition of tumor growth by leveraging the collaborative effects of the therapies. The findings of this study introduce a novel and promising strategy for the integrative and collaborative treatment of refractory cancers, providing valuable insights into addressing the challenges posed by aggressive breast cancer, particularly TNBC.