bluuebunny/crossref_metadata_2025_split huggingface dataset:
Clinical characteristics and in-hospital management strategies in patients with acute coronary syndrome: results from 2,096 accredited Chest Pain Centers in China from 2016 to 2021
Authors: Li Linjie , Zhou Xin , Jin Zhengyang , A Geru , Sun Pengfei , Wang Zhuoqun , Li Yongle , Xu Chengyi , Su Xi , Yang Qing , Huo Yong 
Published: January 2023

Background and purpose:
The Chest Pain Center accreditation project was launched in 2011 in China as a nationwide effort to improve clinical management of acute chest pain patients. In this study, we summarize the clinical characteristics and in-hospital outcomes of patients undergoing treatment for acute coronary syndrome (ACS) in Chest Pain Centers in China.

Methods:
Data were based on the Chinese Cardiovascular Association (CCA) Database-Chest Pain Center of 1,745,118 ACS patients admitted at 2,096 accredited Chest Pain Center between January 1, 2016, and December 31, 2021. Patient characteristics, time delays, treatment, and outcomes were analyzed using descriptive analysis.

Results:
The final analysis included a total of 1,745,118 patients, 699,476 patients (40.1%) with ST segment elevation myocardial infarction (STEMI), 349,572 (20.0%) with non-ST segment elevation myocardial infarction (NSTEMI), and 696,070 (39.9%) with unstable angina (UA). Electrocardiogram (ECG) was conducted in 89.4% of the patients within 10 min after first medical contact. For STEMI patients, the median door-to-wire crossing time was 72.1 (53.1 to 91.9) min and the median first medical contact-to-needle time was 32.3 (23.8 to 58.6) min. In-hospital mortality was 2.0% in the overall analysis, 3.6% for STEMI, 2.1% for NSTEMI, and 0.3% for UA. Primary percutaneous coronary intervention (PCI) was conducted in 62.8% of STEMI patients, with increasing rate in grade I and II hospitals over the 6-year study period. Patients treated with thrombolysis had significantly higher mortality than those treated with PCI and thrombolysis combined with PCI. The development of Chest Pain Centers varied substantially across geographic regions.

Conclusions:
Based on CCA Database-Chest Pain Center, the current study provided an overall description of the clinical characteristics of ACS patients in China. The results on management pattern and in-hospital outcomes of STEMI patients identified important areas for further improvement in ACS patient management in China.
Age and Mental Health Symptoms Among Chinese Persons With HIV: The Mediating and Moderating Role of Perceived Discrimination
Authors: Han Shuyu , Pei Yaolin , Wang Jianyun , Hu Yan , Zhu Zheng , Qi Xiang , Yang Zhongfang , Wu Bei 
Published: January 2023

Abstract
The association between age and mental health symptoms among persons with HIV (PWH) is inconsistent, and little is known about the mediators and moderators of this association. This study aimed to examine the association between age and mental health symptoms, as well as the mediators and moderators of perceived discrimination. Data were from 1,304 PWH who completed a cross-sectional survey in five areas of China. Multiple linear regressions showed that younger age was significantly associated with more severe mental health symptoms and that perceived discrimination moderated this relationship. The Sobel test showed that perceived discrimination also mediated the association between age and mental health symptoms. Our study indicates that perceived discrimination shapes the association between age and mental health symptoms among PWH and highlights the importance of designing age-tailored mental health intervention strategies for perceived discrimination among young PWH. Interventions addressing discrimination are necessary to help improve mental health, especially for young PWH.
Nurse-facilitated preexposure prophylaxis delivery for adolescent girls and young women seeking contraception at retail pharmacies in Kisumu, Kenya
Authors: Pintye Jillian , Odoyo Josephine , Nyerere Bernard , Achieng Pauline , Araka Evelyne , Omondi Christine , Ortblad Katrina F. , Mugambi Melissa L. , Baeten Jared M. , Bukusi Elizabeth A. 
Published: January 2023

Objective:
We evaluated preexposure prophylaxis (PrEP) uptake, initiation, and continuation within a nurse-facilitated pharmacy-based delivery model for Kenyan adolescent girls and young women (AGYW) seeking contraception at retail pharmacies.

Methods:
From October 2020 to March 2021, PrEP-trained nurses were stationed at three retail pharmacies in Kisumu, Kenya. AGYW (aged 15–24 years) purchasing contraception (emergency contraception, oral contraceptive pills, injectables, implants, condoms) were counseled on PrEP, completed HIV testing, and offered a free 1-month supply of PrEP pills per national guidelines by nurses under supervision of a remote physician. We evaluated uptake among all AGYW offered PrEP. At 30 days after uptake, we evaluated PrEP use initiation and plans for continuation.

Results:
We enrolled 235 AGYW clients who were HIV-negative and purchasing contraception at pharmacies. Emergency contraception was the most frequently purchased contraceptive (35%). Median age was 22 years (IQR 19–23), 44% were currently in school, and 33% currently had multiple sexual partners. One-fourth (24%) exchanged sex for money or favors and 14% had sex while intoxicated in the prior 6 months. Overall, PrEP uptake was 85%; at 1 month, 82% had initiated PrEP use and 68% planned to continue use. Among those initiating PrEP, 69% were willing to pay for PrEP at retail pharmacies (median KES 150, IQR 100–200) even if available for free at public sector facilities.

Conclusion:
In this evaluation of nurse-facilitated PrEP delivery at pharmacies in Kenya, a substantial proportion of AGYW who purchased contraception subsequently initiated PrEP, planned to continue use, and were willing to pay for PrEP.
Variation in hospice use among trauma centers may impact analysis of geriatric trauma outcomes: An analysis of 1,961,228 Centers for Medicare and Medicaid Services hospitalizations from 2,317 facilities
Authors: Fakhry Samir M. , Shen Yan , Wyse Ransom J. , Garland Jeneva M. , Watts Dorraine D. 
Published: January 2023

BACKGROUND
Defining discharges to hospice as “deaths” is vital for properly assessing trauma center outcomes. This is critical with older patients as a higher proportion is discharged to hospice. The goals of this study were to measure rates of hospice use, evaluate hospice discharge rates by trauma center level, and identify variables affecting hospice use in geriatric trauma.

METHODS
Patients from the Centers for Medicare and Medicaid Services Inpatient Standard Analytical Files for 2017 to 2019, 65 years or older, with ≥1 injury International Classification of Diseases, Tenth Revision, code, at hospitals with ≥50 trauma patients per year were selected. Total deaths was defined as inpatient deaths plus hospice discharges. Dominance analysis identified the most important contributors to a model of hospice use.

RESULTS
A total of 1.96 million hospitalizations from 2,317 hospitals (Level I, 10%; II, 14%; III, 18%; IV, 7%; none, 51%) were included. Level I's had significantly lower raw hospice discharge values compared with Levels II and III (I, 0.030; II, 0.035; III, 0.035; p < 0.05) but not Level IV (0.032) or nontrauma centers (0.030) (p > 0.05). Adjusted Level I hospice discharge rates were lower than all other facility types (Level I, 0.026; II, 0.031; III, 0.034; IV, 0.033; nontrauma, 0.030; p < 0.05). Hospice discharges as a proportion of total deaths varied by level and were lowest (0.38) at Level I centers. Dominance analysis showed that proportion of patients with Injury Severity Score of >15 contributed most to explaining hospice utilization rates (3.2%) followed by trauma center level (2.3%), proportion White (1.9%), proportion female (1.5%), and urban/rural setting (1.4%).

CONCLUSION
In this near population-based geriatric trauma analysis, Level I centers had the lowest hospice discharge rate, but hospice discharge rates varied significantly by trauma level and should be included in mortality assessments of hospital outcomes. As the population ages, accurate assessment of geriatric trauma outcomes becomes more critical. Further studies are needed to evaluate the optimal utilization of hospice in end-of-life decision making for geriatric trauma.

LEVEL OF EVIDENCE
Therapeutic/Care Management; Level II.
Vibrio sinus sp. nov., a marine bacterium isolated from coastal seawater
Authors: Wang Wei , Zhao Wenbin , Cao Bingbing , Liu Ronghua , Cheng Haojin , Tian Peng , Xiao Jiaguang , Xu Ziqing , Wang Xiaolei , Niu Wentao 
Published: January 2023

A Gram–straining–negative, facultatively anaerobic, motile by means of a polar flagellum and rod-shaped marine bacterium, designated S4M6T, was isolated from surface seawater collected in Dongshan Bay (Fujian, PR China). Phylogenetic analysis based on 16S rRNA genes, phylogenomic analysis of single-copy gene families and whole genome data indicated that S4M6T represented a member of the genus

Vibrio

. The closest phylogenetic relatives of S4M6T were

Vibrio marisflavi

CGMCC 1.8994T (97.8 % 16S rRNA gene sequence pairwise similarity),

Vibrio variabilis

LMG 25438T (96.9 %),

Vibrio gangliei

SZDIS-1T (96.2 %) and

Vibrio aestivus

M22T (96.1 %). The growth of S4M6T occurred at 15–35 °C (optimum 28 °C), pH 4.0–9.0 (optimum 5.0–7.0) and in the presence of 2–5 % (w/v) NaCl (optimum 3 %). The predominant fatty acids (>10 %) are C16 : 0, summed feature 3 (C16 : 1ω7c and/or C16 :
1ω6c) and summed feature 8 (C18 : 1ω7c and/or C18 :
1ω6c). The DNA G+C content of the assembled genomic sequences was 43.4 % for S4M6T. Average nucleotide identity (ANI) values between S4M6T and the reference species were lower than the threshold for species delineation (95–96 %); in silico DNA–DNA hybridization further indicated that S4M6T had less than 70 % similarity to its relatives. On the basis of the polyphasic evidence, strain S4M6T is proposed to represent a novel species of the genus

Vibrio

, for which the name Vibrio sinus sp. nov. is proposed. The type strain is S4M6T (= KCTC 92312T= MCCC 1K06167T).
HPV and p16 expression association with 5-year survival in oral squamous cell carcinoma patients of North-East India
Authors: Das Rajjyoti , Kumar Rupesh , Rai Avdhesh Kumar , Sarma Anupam , Kakoti Lopamudra , Kataki Amal Chandra , Bhattacharyya Mouchumee , Kalita Manoj 
Published: January 2023

ABSTRACTBackgroundIn our study, we examined the 5-year survival of OSCC patients with HPV positive or negative status along with p16 protein expression.MethodA total of 72 biopsy tissue specimens from histologically confirmed oral squamous cell carcinoma (OSCC) patients were collected. HPV detection and genotyping were performed using HPV E6/E7 and HPV-type specific multiplex primer for nested-PCR. Immunohistochemistry evaluation of pl6 was conducted. SPSS statistical software (ver 20) was used for data analysis.ResultsHigh risk-HPV (hr-HPV) DNA positivity was found in 27.7% (n=20) of OSCC patients. Stage III OSCC patients were 7.80 times more likely to survive 5 years than stage IV patients (OR-7.80 CI-95%; P-0.03). Among the hr-HPV positive OSCC patients, we found that the median survival time for the 1st year (95%), 3 years (78.5%), and 5 years (38.5%) was significantly higher than that of the hr-HPV negative [1st year (78.6%), 3 years (45.2%) and 5 years (38.5%)] OSCC patients (P-0.03 The survival of male patients with hr-HPV positive OSCC is 9.75 times greater than the survival of patients with HPV negative OSCC (OR-9.75; CI-95%; P-0.05). The p16 expression level (low to overexpression) group and negative P16 expression group of OSCC patients have not demonstrated a significant association with 5-year survival.ConclusionWe conclude that in OSCC cases of North-East India, the presence of hr-HPV in OSCC cases could be a good predictor of 5-year survival rate. Expression of p16 does not appear to have any significant association with 5-year survival.
The role of rare genetic variants enrichment in epilepsies of presumed genetic etiology
Authors: Bundalian Linnaeus , Su Yin-Yuan , Chen Siwei , Velluva Akhil , Kirstein Anna Sophia , Garten Antje , Biskup Saskia , Battke Florian , Lal Dennis , Heyne Henrike O. , Platzer Konrad , Lin Chen-Ching , Lemke Johannes R. , Duc Diana Le ,   Epi25 Collaborative
Published: January 2023

1.AbstractPrevious studies suggested that severe epilepsies e.g., developmental and epileptic encephalopathies (DEE) are mainly caused by ultra-rarede novogenetic variants. For milder phenotypes, rare genetic variants could contribute to the phenotype. To determine the importance of rare variants for different epilepsy types, we analyzed a whole-exome sequencing cohort of 9,170 epilepsy-affected individuals and 8,436 controls. Here, we separately analyzed three different groups of epilepsies : severe DEEs, genetic generalized epilepsy (GGE), and non-acquired focal epilepsy (NAFE). We required qualifying rare variants (QRVs) to occur in controls at a minor allele frequency ≤ 1:1,000, to be predicted as deleterious (CADD≥20), and to have an odds ratio in epilepsy cases ≥2. We identified genes enriched with QRVs in DEE (n=21), NAFE (n=72), and GGE (n=32) - the number of enriched genes are found greatest in NAFE and least in DEE. This suggests that rare variants may play a more important role for causality of NAFE than in DEE. Moreover, we found that QRV-carrying genes e.g.,HSGP2, FLNAorTNCare involved in structuring the brain extracellular matrix. The present study confirms an involvement of rare variants for NAFE, while in DEE and GGE, the contribution of such variants appears more limited.
Genome diversity ofLeishmania aethiopica
Authors: Hadermann Amber , Heeren Senne , Maes Ilse , Dujardin Jean-Claude , Domagalska Malgorzata Anna , Van den Broeck Frederik 
Published: January 2023

ABSTRACTLeishmania aethiopicais a zoonotic Old World parasite transmitted by Phlebotomine sand flies and causing cutaneous leishmaniasis in Ethiopia and Kenya. Despite a range of clinical manifestations and a high prevalence of treatment failure,L. aethiopicais the most neglected species of theLeishmaniagenus in terms of scientific attention. Here, we explored the genome diversity ofL. aethiopicaby analyzing the genomes of twenty isolates from Ethiopia. Phylogenomic analyses identified two strains as interspecific hybrids involvingL. aethiopicaas one parent andL. donovaniandL. tropicarespectively as the other parent. High levels of genome-wide heterozygosity suggest that these two hybrids are equivalent to F1 progeny that propagated mitotically since the initial hybridization event. Analyses of allelic read depths further revealed that theL. aethiopica-L. tropicahybrid was diploid and theL. aethiopica-L. donovanihybrid was triploid, as has been described for other interspecificLeishmaniahybrids. When focusing onL. aethiopica, we show that this species is genetically highly diverse and consists of both asexually evolving strains and groups of recombining parasites. A remarkable observation is that someL. aethiopicastrains showed an extensive loss of heterozygosity across large regions of the chromosomal genome, which likely arose from gene conversion/mitotic recombination. Hence, our prospection ofL. aethiopicagenomics revealed new insights into the genomic consequences of both meiotic and mitotic recombination inLeishmania.
Development of a Novel Medium Throughput Flow-Cytometry Based Micro-Neutralisation Test for SARS-CoV-2 with Applications in Clinical Vaccine Trials and Antibody Screening
Authors: O’Reilly Sophie , Kenny Grace , Alrawahneh Tamara , Francois Nathan , Angeliadis Matthew , de Masson d’Autume Valentin , Leon Alejandro Garcia , Feeney Eoin R. , Yousif Obada , Cotter Aoife , de Barra Eoghan , Horgan Mary , Mallon Patrick WG , Gautier Virginie 
Published: January 2023

AbstractQuantifying neutralising capacity of circulating SARS-COV-2 antibodies is critical in evaluating protective humoral immune responses generated post-infection/post-vaccination. Here we describe a novel medium-throughput flow cytometry-based micro-neutralisation test to evaluate Neutralising Antibody (NAb) responses against live SARS-CoV-2 Wild Type and Variants of Concern (VOC) in convalescent/vaccinated populations. Flow Cytometry-Based Micro-Neutralisation Test (Micro-NT) was performed in 96-well plates using clinical isolates WT-B, WT-B.177.18 and/or VOCs Beta and Omicron. Plasma samples (All Ireland Infectious Diseases (AIID) Cohort) were serially diluted (8 points, half-log) from 1/20 and pre-incubated with SARS-CoV-2 (1h, 37°C). Virus-plasma mixture were added onto VERO E6/VERO E6 TMPRSS2 cells for 18h. Percentage infected cells was analysed by automated flow cytometry following trypsinisation, fixation and SARS-CoV-2 Nucleoprotein intracellular staining. Half-maximal Neutralisation Titres (NT50) were determined using four-parameter logistic regression. Our assay was compared to Plaque Reduction Neutralisation Test (PRNT) and validated against WHO anti-SARS-CoV-2 Immunoglobulin Standards. Using WHO Standards with low, medium or high anti-SARS-CoV-2 IgG, both Micro-NT and PRNT achieved comparable NT50 values. Micro-NT was found to be highly reproducible (inter-assay CV of 11.64%). Screening 190 convalescent samples and 11 COVID-19 naive controls (AIID cohort) we demonstrated that Micro-NT has broad dynamic range differentiating NT50s <1/20 to >1/5000. We could also characterise immune-escape VOC observing up to 10-fold reduction in NT50 against SARS-CoV-2 Beta variant. Our flow cytometry-based Micro-NT is a robust and reliable assay to quantify NAb titres, and has been selected as an endpoint in clinical trials. It has higher throughput (96 well format versus 12 well) and reduced infection time (18h vs 48-96h) compared to the gold standard PRNT.
How would you respond to stereotype pressure? The effects of perceived occupational stigma on social workers’ proactive behavior in China
Authors: Zhan Jing , Zhao Yue 
Published: January 2023

PurposeThe purpose of this study is to explore a balanced understanding of the relationship between perceived occupational stigma and social workers’ proactive behaviors in China. Drawing on cognitive appraisal theory, this study explored the mediating role of threat and challenge appraisals, as well as the moderating role of trait resilience.Design/methodology/approachData were collected using a time-lagged research design. The hypotheses were examined using a sample of 338 social workers in China.FindingsPerceived occupational stigma is appraised as both a challenge and a threat simultaneously. Challenge appraisal positively mediated perceived occupational stigma and proactive behavior, whereas threat appraisal negatively mediated this relationship. Trait resilience moderated the effect of threat appraisal, suggesting that perceived occupational stigma was appraised as a threat when trait resilience was lower (rather than higher), which then reduced social workers’ proactive behaviors.Originality/valueThis study enriches the literature on perceived occupational stigma by identifying the relationship and mechanism of perceived occupational stigma-proactive behaviors and a boundary condition from the theoretical perspective of cognitive appraisals. It demonstrates both the positive and negative aspects of perceived occupational stigma as appraised by social workers in relation to an important workplace outcome of proactive behaviors. In addition, it offers a fresh approach by exploring perceived occupational stigma from the perspective of social workers.
Entrepreneurial growth aspirations at re-entry after failure
Authors: Fuentelsaz Lucio , González Consuelo , Mickiewicz Tomasz 
Published: January 2023

PurposeUtilising the Theory of Planned Behaviour as the conceptual framework, the authors argue that entrepreneurial financial failure enhances entrepreneurial growth aspirations for the subsequent start-up projects. Furthermore, this effect is particularly strong for individuals rich in human capital, both general and specific; for them, financial failure of an entrepreneurial business is likely to be subsequently transformed into higher entrepreneurial growth aspirations.Design/methodology/approachThe authors employ multilevel estimation techniques applied to Global Entrepreneurship Monitor data consisting of annual subsamples, each with at least 2,000 observations drawn from the working age population of 95 countries, for the period 2007–2019.FindingsThe results confirm that the experience of financial failure, both individual and societal, leads to higher growth aspirations for subsequent ventures, while exit for opportunity reasons has an even stronger positive effect on growth aspirations. Furthermore, higher education and entrepreneurial experience enhance the positive impact of financial failure on the growth aspirations of subsequent start-ups.Originality/valueThe authors demonstrate that the Theory of Planned Behaviour, which centres on intentions, can be successfully utilised to understand why entrepreneurial failure may be transformed into high growth aspirations for subsequent projects and why this effect may be enhanced by the human capital of the entrepreneur. Furthermore, the authors apply multilevel methods to a large international dataset from Global Entrepreneurship Monitor and produce novel empirical evidence supporting their theoretical predictions.
How do Pakistani drama serials discursively represent domestic violence?
Authors: Ahmad Faiqa 
Published: January 2023

PurposeThe objective of this study was to look closely at how domestic violence is represented in Pakistani drama serials to see if portrayals are reinforcing stereotypical and/or patriarchal values, or breaking the rigid norms.Design/methodology/approachWith the help of dispositive analysis within the critical discourse approach, the prominent and non-dominant discourses about domestic violence were identified and discussed. Episodes from two popular drama serials, Kaisa Yeh Naseeban and Khaas, released in 2019, were watched with special focus on texts on domestic violence alongside objects and actions.FindingsAnalysis showed that both drama serials gave importance to socio-systemic and liberal humanist instrumentalism discourses, which describe domestic violence as a result of social structures and that abuse is used to assert control, respectively. However, some instances were noted where patriarchal values were encouraged.Originality/valueAs media has become a powerful tool of influence and awareness in the recent times, it is imperative that the content watched on it by millions of people be studied and analyzed. It is claimed that Pakistani drama serials with wide following and that are made on social issues around women aim to raise awareness and empower them. Domestic violence is a prevalent issue in Pakistan, and no research till date has examined representation of domestic violence on Pakistani popular media, which may influence response to domestic violence, which this paper aims to do.