bluuebunny/crossref_metadata_2025_split huggingface dataset:
Systematic Quality Evaluation of Epimedium wushanense T. S. Ying Based on Two Quality Control Standards: Total Flavonoid Glycosides and Epimedin C
Authors: Wang Congying , Tian Shuyun , Tang Qin , Zhou Ziwei , Peng Xinheng , Cai Xiaoxue , Xu Yanqin 
Published: February 2023

AbstractTwo quality control standards, total flavonoid glycosides of Epimedii Folium and epimedin C of Epimedii Wushanensis Folium, were used to systematically evaluate the quality of Epimedium wushanense T. S. Ying, so as to provide reference for its germplasm screening and resource utilization. Seven representative populations of E. wushanense covering its main distribution areas were uniformly sampled during the flowering period. There were significant quality differences among the populations of E. wushanense. According to the quality standard of total flavonoid glycosides, all populations were superior to the quality standard of the Chinese Pharmacopoeia for Epimedii Folium, with more than 1.5 % total flavonoid glycosides. The variation ranges of epimedin A, epimedin B, epimedin C, icariin and total flavonoid glycosides were 0.40–0.76 %, 0.51–0.83 %, 1.70–9.31 %, 0.40–1.23 % and 3.05–10.61 %, respectively. According to the quality standard of epimedin C, all populations were better than the quality standard of the Chinese Pharmacopoeia for Epimedii Wushanensis Folium, with more than 1.0 % epimedin C. The variation range of epimedin C was 2.22–10.06 %. When comparing the results of the two methods, a trend of slightly lower mean values was found for total flavonoid glycosides, except for the HBXW population. The quality of E. wushanense was superior to both the quality standard of Epimedii Folium and Epimedii Wushanense Folium in the Chinese Pharmacopoeia. Epimedin C was the most abundant component. Among the investigated populations, HBXW and HBGK exhibited the highest quality, and may provide excellent genetic resources for standardized cultivation. In addition, the habitat of these populations can also serve a reference for cultivation conditions.
Early healing after treatment of coronary lesions by thin strut everolimus, or thicker strut biolimus eluting bioabsorbable polymer stents: The SORT‐OUT VIII OCT study
Authors: Andreasen Lene N. , Balleby Ida R. , Barkholt Trine Ø. , Hebsgaard Lasse , Terkelsen Christian J. , Holck Emil N. , Jensen Lisette O. , Maeng Michael , Dijkstra Jouke , Antonsen Lisbeth , Kristensen Steen D. , Tu Shengxian , Lassen Jens F. , Christiansen Evald H. , Holm Niels R. 
Published: February 2023

AbstractAimsEarly healing after drug‐eluting stent (DES) implantation may reduce the risk of stent thrombosis. The aim of this study was to compare patterns of early healing after implantation of the thin strut everolimus‐eluting Synergy DES (Boston Scientific) or the biolimus‐eluting Biomatix Neoflex DES (Biosensors).Methods and ResultsA total of 160 patients with the chronic or acute coronary syndrome were randomized 1:1 to Synergy or Biomatrix DES. Optical coherence tomography (OCT) was performed at baseline and at either 1‐ or 3‐month follow‐up. The primary endpoint was a coronary stent healing index (CSHI), a weighted index of strut coverage, neointimal hyperplasia, malapposition, and extrastent lumen. A total of 133 cases had OCT follow‐up and 119 qualified for matched OCT analysis. The median CSHI score did neither differ significantly between the groups at 1 month: Synergy 8.0 (interquartile range [IQR]: 3.0; 14.0) versus Biomatrix 8.5 (IQR: 4.0; 15.0) (p = 0.47) nor at 3 months: Synergy 6.5 (IQR: 2.0; 13.0) versus Biomatrix 6.0 (IQR: 4.0; 11.0) (p = 0.83). Strut coverage was 84.6% (IQR: 72.0; 97.9) for Synergy versus 77.6% (IQR: 70.1; 90.3) for Biomatrix (p = 0.15) at 1 month and 90.3% (IQR 79.0; 98.8) (Synergy) versus 83.9% (IQR: 77.5; 92.6) (Biomatrix) (p = 0.068) at 3 months. Pooled 1‐ and 3‐month coverage was 88.6% (IQR: 74.4; 98.4) for Synergy compared with 80.7% (IQR: 73.2; 90.8) for Biomatrix (p = 0.02).ConclusionsThe early healing response after treatment with the Synergy or Biomatrix DES did not differ significantly as determined by a healing index. The Synergy DES showed overall better early stent strut coverage.
Influence of the Ageing and Drying Steps of a CoMoP/γ‐Al2O3 Catalyst onto the Multi‐Scale Molybdenum Active Phase Organization
Authors: Cottrez Candice , Humbert Séverine , Gay Anne‐Sophie , Devers Elodie , De Geuser Frédéric , Carrier Xavier , Chaumonnot Alexandra 
Published: February 2023

AbstractTo study the influence of ageing and drying steps onto the multiscale Mo active phase arrangement (i. e. MoS2 slabs and slab aggregates), a series of highly loaded CoMoP/γ‐Al2O3 catalysts aged, unaged, dried or freeze‐dried were prepared and tested in toluene hydrogenation. Electron Probe Micro Analyses (EPMA) and Anomalous Small Angle X‐Ray Scattering (ASAXS) permit to highlight the influence of each preparation steps. A growth of Mo entities occurs during ageing along with metal diffusion inside the support and leads to longer slabs. The same occurs during drying with metal redistribution due to solvent evaporation. Concomitantly, a fragmentation of slab aggregates takes place and leads to numerous small slabs aggregates. Slab length and aggregates amount were found to drive the catalytic activity, which translates the relationship between activity, number of active sites and their accessibility. Conversely, macroscopic metal distribution into the extrudates appears to have no impact on the catalytic performance.
An Atropisomerism Study of Large Cycloarylenes: [n]Cyclo‐4,10‐Pyrenylenes’ Case
Authors: Kurosaki Ryo , Morimoto Hirofumi , Matsuo Kyohei , Hayashi Hironobu , Yamada Hiroko , Aratani Naoki 
Published: February 2023

AbstractAn atropisomerism of large cycloarylenes was studied using [n]cyclo‐4,10‐pyrenylenes (n=6–21) as an illustrative example with two simple assumptions: (1) alternating configurations (R,S,R,S,…) are thermodynamically most stable, and (2) three consecutive identical configurations (R,R,RorS,S,S) are prevented. Ni‐mediated coupling of a 5,9‐diiodopyrene gave a series of directly‐linked cyclic pyrene oligomers in one‐pot reaction. As‐synthesized cyclic hexamer was assigned as an (R,S,S,R,R,S) structure, converted into an (R,S,R,S,R,S)‐form upon heating. Cyclic heptamer consists of two types ofC2symmetric structures predicted from assumption (2), one of which was convergent to one another by heating. Three atropisomers of cyclic octamer were analyzed from the possible five candidates by means of1H nuclear magnetic resonance (NMR) spectroscopy, and the conversion process to (R,S,R,S,R,S,R,S) configurations upon heating was investigated. In total, according to two simple rules, the analysis of atropisomerism could be performed smoothly.
Absolute Configuration Determination of Chiral API Molecules by MicroED Analysis of Cocrystal Powders Formed Based on Cocrystal Propensity Prediction Calculations**
Authors: Shah Harsh S. , Yuan Jiuchuang , Xie Tian , Yang Zhuocen , Chang Chao , Greenwell Chandler , Zeng Qun , Sun GuangXu , Read Brandon N. , Wilson Timothy S. , Valle Henry U. , Kuang Shanming , Wang Jian , Sekharan Sivakumar , Bruhn Jessica F. 
Published: February 2023

AbstractEstablishing the absolute configuration of chiral active pharmaceutical ingredients (APIs) is of great importance. Single crystal X‐ray diffraction (scXRD) has traditionally been the method of choice for such analysis, but scXRD requires the growth of large crystals, which can be challenging. Here, we present a method for determining absolute configuration that does not rely on the growth of large crystals. By examining microcrystals formed with chiral probes (small chiral compounds such as amino acids), absolute configuration can be unambiguously determined by microcrystal electron diffraction (MicroED). Our streamlined method employs three steps: (1) virtual screening to identify promising chiral probes, (2) experimental cocrystal screening and (3) structure determination by MicroED and absolute configuration assignment. We successfully applied this method to analyze two chiral API molecules currently on the market for which scXRD was not used to determine absolute configuration.
Styrene Oxide Isomerase‐Catalyzed Meinwald Rearrangement in Cascade Biotransformations: Synthesis of Chiral and/or Natural Chemicals
Authors: See Willy W. L. , Li Zhi 
Published: February 2023

AbstractStyrene oxide isomerase (SOI) catalyzes the Meinwald rearrangement of aryl epoxides to carbonyl compounds via a 1,2‐trans‐shift in a stereospecific manner. A number of cascade biotransformations with SOI‐catalyzed epoxide isomerization as a key step have been developed to convert readily available substrates into valuable chiral chemicals. Cascade conversion of terminal or internal alkenes into chiral acids, alcohols or amines was achieved, which involved SOI‐catalyzed enantio‐retentive isomerization of terminal epoxides via 1,2‐H shift, or internal epoxides via 1,2‐methyl shift. SOI‐involved cascades were also developed to convert racemic epoxides into chiral acids or amines via dynamic kinetic resolution. Additionally, combining SOI‐catalyzed isomerization with enantioselective C−C bond forming enzymes enabled the synthesis of chiral amino acids or amino alcohols from racemic epoxides. Finally, integration of SOI‐involved cascades with biosynthesis pathways allowed for the direct utilization of renewable substrates for the sustainable synthesis of high‐value natural chemicals such as alcohols, acids, and esters.
Taste arbor structural variability analyzed across taste regions
Authors: Ohman Lisa C. , Hanbali Lama , Krimm Robin F. 
Published: February 2023

AbstractTaste ganglion neurons are functionally and molecularly diverse, but until recently morphological diversity was completely unexplored. Specifically, taste arbors (the portion of the neuron within the taste bud) vary in structure, but the reason for this variability is unclear. Here, we analyzed structural variability in taste arbors to determine which factors determine their morphological diversity. To characterize arbor morphology and its relationship to taste bud cells capable of transducing taste stimuli (taste‐transducing cell) number and type, we utilized sparse cell genetic labeling of taste ganglion neurons in combination with whole‐mount immunohistochemistry. Reconstruction of 151 taste arbors revealed variation in arbor size, complexity, and symmetry. Overall, taste arbors exist on a continuum of complexity, cannot be categorized into discrete morphological groups, and do not have stereotyped endings. Arbor size/complexity was not related to the size of the taste bud in which it was located or the type of taste‐transducing cell contacted (membranes within 180 nm). Instead, arbors could be broadly categorized into three groups: large asymmetrical arbors contacting many taste‐transducing cells, small symmetrical arbors contacting one or two taste‐transducing cells, and unbranched arbors. Neurons with multiple arbors had arbors in more than one of these categories, indicating that this variability is not an intrinsic feature of neuron type. Instead, we speculate that arbor structure is determined primarily by nerve fiber remodeling in response to cell turnover and that large asymmetrical arbors represent a particularly plastic state.
Metal Doping to Control Gate Opening and Increase Methane Working Capacity in Isostructural Flexible Diamondoid Networks
Authors: Wang Shao‐Min , Shivanna Mohana , Lama Prem , Yang Qing‐Yuan , Barbour Leonard J. , Zaworotko Michael J. 
Published: February 2023

AbstractAdsorbed natural gas (ANG) systems involve using porous materials to increase the working capacity and/or reduce the storage pressure compared to compressed natural gas (CNG). Flexible metal‐organic materials (FMOMs) are particularly interesting in this context since their stepped isotherms can afford increased working capacity if the adsorption/desorption steps occur within the proper pressure range. We report herein that metal doping in a family of isostructural FMOMs, ML2 (M=Co, Ni or NixCo1−x, L=4‐(4‐pyridyl)‐biphenyl‐4‐carboxylic acid), enables control over the gate opening between non‐porous (closed) and porous (open) phases at pressures relevant to methane storage. Specifically, methane‐induced phase transformations can be fine‐tuned by using different Ni/Co ratios to enhance methane working capacity. The optimal working capacity from 5 to 35 bar at 298 K (153 cm3 cm−3) was found for Ni0.89Co0.11L2 (X‐dia‐1‐Ni0.89Co0.11), which is greater than that of benchmark rigid MOFs.
Synthesis, antitubercular evaluation, and molecular docking studies of hybrid pyridinium salts derived from isoniazid
Authors: Bhavani Gollagani Vijaya , Kondapuram Sree Karani , Shamsudeen Aifa Fathima , Coumar Mohane Selvaraj , Selvin Joseph , Kannan Tharanikkarasu 
Published: February 2023

AbstractIn the quest to develop potent inhibitors for Mycobacterium tuberculosis, novel isoniazid‐based pyridinium salts were designed, synthesized, and tested for their antimycobacterial activities against the H37Rv strain of Mycobacterium tuberculosis using rifampicin as a standard. The pyridinium salts 4k, 4l, and 7d showed exceptional antimycobacterial activities with MIC90 at 1 µg/mL. The in vitro cytotoxicity and pharmacokinetics profiles of these compounds were established for the identification of a lead molecule using in vivo efficacy proof‐of‐concept studies and found that the lead compound 4k possesses LC50 value at 25 µg/mL. The in vitro antimycobacterial activity results were further supported by in silico studies with good binding affinities ranging from −9.8 to −11.6 kcal/mol for 4k, 4l, and 7d with the target oxidoreductase DprE1 enzyme. These results demonstrate that pyridinium salts derived from isoniazid can be a potentially promising pharmacophore for the development of novel antitubercular candidates.
A novel FAME1 repeat configuration in a European family identified using a combined genomics approach
Authors: Maroilley Tatiana , Tsai Meng‐Han , Mascarenhas Rumika , Diao Catherine , Khanbabaei Maryam , Kaya Sabine , Depienne Christel , Tarailo‐Graovac Maja , Klein Karl Martin 
Published: February 2023

AbstractFamilial adult myoclonic epilepsy (FAME) is an adult‐onset neurological disease characterized by cortical tremor, myoclonus, and seizures due to a pentanucleotide repeat expansion: a combination of pathogenic TTTCA expansion associated with a TTTTA repeat in introns of six different genes. Repeat‐primed PCR (RP‐PCR) is an inexpensive test for expansions at known loci. The analysis of the SAMD12 locus revealed that the repeats have different size, configuration, and composition. The TTTCA repeats can be very long (>1000 repeats) but also very short (14 being the shortest identified). Here, we report siblings of European descent with the clinical diagnosis of FAME yet a negative RP‐PCR test. Using short‐read genome sequencing, we identified the pentanucleotide expansion in intron 4 of SAMD12, which was confirmed by CRIPSR‐Cas9‐mediated enrichment and long‐read sequencing to be of (TTTTA)~879(TTTCA)3(TTTTA)7(TTTCA)7 configuration. Our finding is the first to associate the SAMD12 locus in European patients with FAME and currently represents the shortest identified TTTCA expansion. Our results suggest that the SAMD12 locus should be tested in patients with suspected FAME independent of ethnicity. Furthermore, RP‐PCR may miss the underlying mutation, and genome sequencing may be needed to confirm the pathogenic repeat.
Shaking table test of a damped‐outrigger system incorporating friction dampers
Authors: Lin Pao‐Chun , Chen Ming‐Ching , Chung Meng‐Lin 
Published: February 2023

AbstractThe main purpose of this study is to investigate the seismic performance of a damped‐outrigger system incorporating friction dampers through numerical analysis and shaking table tests. A 9‐m‐tall steel structure specimen was designed by scaling down a 20‐story benchmark model. The specimen was equally divided into 10 floors; the outrigger beams together with the friction dampers could be installed in different floors. The normal force in the friction damper was adjustable so that its energy performance could be modified during the test. The seismic response of the specimen was evaluated by performing response spectral analysis (RSA) using the OpenSees numerical model. The equivalent damping ratio was included in the RSA to evaluate the energy dissipation resulting from the friction dampers. Based on the RSA results, the specimen configurations when outrigger was located at the sixth (6F), eighth (8F), and roof floors (RF) and when the normal force in the friction damper varied between 5, 10, and 20 kN were tested by imposing five different ground motions with the peak ground acceleration of 0.64g. Both the RSA and test results indicated that the maximum roof drift of the specimen was around 0.7, 0.4, and 0.3% rad., when the outrigger was located at the RF, 8F, and 6F, respectively. The greater normal force applied in the friction damper generally result in a greater amount of energy dissipation and a smaller roof drift response. Based on the experimental and numerical results, the optimal design of the damped‐outrigger system incorporating friction dampers are demonstrated in this study.
Recent advances in targeted antibacterial therapy basing on nanomaterials
Authors: Geng Zhongmin , Cao Zhenping , Liu Jinyao 
Published: February 2023

AbstractBacterial infection has become one of the leading causes of death worldwide, particularly in low‐income countries. Despite the fact that antibiotics have provided successful management in bacterial infections, the long‐term overconsumption and abuse of antibiotics has contributed to the emergence of multidrug resistant bacteria. To address this challenge, nanomaterials with intrinsic antibacterial properties or that serve as drug carriers have been substantially developed as an alternative to fight against bacterial infection. Systematically and deeply understanding the antibacterial mechanisms of nanomaterials is extremely important for designing new therapeutics. Recently, nanomaterials‐mediated targeted bacteria depletion in either a passive or active manner is one of the most promising approaches for antibacterial treatment by increasing local concentration around bacterial cells to enhance inhibitory activity and reduce side effects. Passive targeting approach is widely explored by searching nanomaterial‐based alternatives to antibiotics, while active targeting strategy relies on biomimetic or biomolecular surface feature that can selectively recognize targeted bacteria. In this review article, we summarize the recent developments in the field of targeted antibacterial therapy based on nanomaterials, which will promote more innovative thinking focusing on the treatment of multidrug‐resistant bacteria.
From salt pan to saucepan: Salicornia, a halophytic vegetable with an array of potential health benefits
Authors: Ekanayake Sadini , Egodawatta Chaminda , Attanayake Renuka N. , Perera Dinum 
Published: February 2023

AbstractDomestication of novel crops from the wild is a key process that ensures food security by addressing adaptability to changing environments. These wild plants offer broader and unique health benefits beyond basic nutrition. Salinization resulted in a drastic reduction of arable land and freshwater intended for cultivation. Thus, the global food production is currently under a risk of meeting the demands. Salt‐tolerant plants (i.e., halophytes) are potential food candidates that can be used to increase the productivity of salinized land and also to enhance the diversification of food production. Salicornia can survive under hypersaline and extreme climatic conditions and deliver tender shoots and dried seeds that can be used as a food with a unique taste, texture, and therapeutic benefits. Although Salicornia is not equally popular among all the food cultures globally, it has been consumed over centuries, and it is now making its way to the shelves of supermarkets in some countries such as the United States, UAE, South Korea, and Mexico. Despite being an underutilized crop, Salicornia possesses an array of chemical compounds or basic derivatives that can enhance antioxidant, anti‐obesity, antidiabetes, anti‐inflammatory, cardioprotective, and neuroprotective properties of human nutrition. The presence of considerable amounts of nutritional elements and various functional bioactive phytochemicals have opened up avenues of using Salicornia as a functional food. The present paper reviews the recognition of Salicornia as a functional food and a potential cash crop in saline agriculture to enhance agricultural productivity and also to diversify the current food supply chain.
Epidemiological and clinical characteristics of central nervous system infections in a tertiary center: A retrospective study
Authors: Gajurel Bikram P. , Giri Subarna , Rayamajhi Shivani , Khanal Niharika , Bishowkarma Sagar , Mishra Aman , Karn Ragesh , Rajbhandari Reema , Ojha Rajeev 
Published: February 2023

AbstractBackground and AimsCentral nervous system (CNS) infection is one of the most common causes of morbidity, mortality, and hospital admission worldwide. The natural history of CNS infection is quite fatal. Early diagnosis and treatment have been proven to have a crucial role in patients' survival. The aim of this study was to identify the epidemiological and clinical patterns of patients diagnosed with CNS infections.MethodsThis study is a retrospective study conducted in a tertiary level hospital in Nepal in which patient diagnosed with CNS infections (September 2019 to 2021) were included. Data were collected and analyzed in SPSS.ResultsThe mean age of the 95 patients included in the study was 45.18 ± 19.56. Meningoencephalitis (n = 44, 46.30%) was the most common infection diagnosed. Patients belonging to the age group 30−60 years had a higher frequency of focal neurological deficit, and other classical clinical features. All the patients who died during the treatment had associated comorbidities but no concurrent infections. Altered sensorium, fever, and headache were the common presenting symptoms in all the recovered patients.ConclusionTo ensure optimum disease outcome, early diagnosis and prompt management are crucial. For this, recognizing the local disease patterns in terms of disease distribution, commonly implicated aetiologies, presenting symptoms, and prognostic factors is of utmost importance.