bluuebunny/crossref_metadata_2025_split huggingface dataset:
Can a specific biobehavioral based therapeutic education program lead to changes in pain perception and brain plasticity biomarkers in chronic pain patients? A study protocol for a randomized clinical trial
Authors: Di Bonaventura Silvia , Fernández Carnero Josué , Ferrer Peña Raúl 
Published: July 2023

AbstractBackgroundChronic pain conditions are complex multifactorial disorders with physical, psychological, and environmental factors contributing to their onset and persistence. Among these conditions, the role of brain-derived neurotrophic factor (BDNF) and the impact of a specific therapeutic education (TE) on pain management have emerged as important areas of research.ObjectiveThe aim of this study is to investigate the effects of a specific type of therapeutic education on pain levels and BDNF concentrations.MethodsIn this single-blind, randomized clinical trial, patients will be randomly assigned to one of two groups: one group will receive education with TE and the other group will receive education without TE. Assessments will be made at baseline, mid-treatment, post-intervention, and at one and eight months.OutcomesThe expected results of this study will shed light on the role of TE in pain management and provide insight into its effects on BDNF levels. The intervention will also provide an opportunity to biochemically measure the plastic changes, thereby improving our understanding of its effects on brain plasticity.ConclusionThis study aims to determine the applicability of therapeutic education as an effective non-pharmacological intervention for the management of chronic pain. The rigorous scientific methods used ensure that the proposed interventions will be clinically applicable across different health care systems.Strengths and limitations of this studyThe study will use a specifically pain oriented methodological approach that intends to go beyond purely informational pain neuroscience education, aiming for a more useful understanding of pain from patients.Incorporating the salutogenic model in therapeutic education will bring a health focused perspective to the research, potentially enriching its interpretive framework helping future interventions.The proposed use of clear and replicable guidelines such as GREET or TIDieR may help in the practical implementation challenge for clinicians, potentially influencing also the study’s reproducibility.While the study plans to assess psychosocial factors, pain intensity levels, and brain plastic changes due to therapeutic education, this comprehensive approach might pose notable methodological challenges.Given the emerging field of brain plasticity related to therapeutic education in chronic pain patients, the limited existing research may influence the interpretive depth of the study’s findings.Protocol versionIn this first version of the protocol dated July 2023, we are recruiting the sample, which is planned to be completed in approximately two months (September 2023) in order to start the trial in October 2023.
Protocol for secondary data analysis of 4 UK cohorts examining youth adversity and mental health in the context of intersectionality
Authors: Hosang Georgina M. , Havers Laura , Shuai Ruichong , Fonagy Peter , Fazel Mina , Morgan Craig , Karamanos Alexis , Fancourt Daisy , McCrone Paul , Smuk Melanie , Bhui Kamaldeep , Shakoor Sania 
Published: July 2023

AbstractBackgroundYouth adversity (e.g., abuse and bullying victimisation) is robust risk factor for later mental health problems (e.g., depression and anxiety). Research shows the prevalence of youth adversity and rates of mental health problems vary by individual characteristics, identity or social groups (e.g., gender and ethnicity). However, little is known about whether the impact of youth adversity on mental health problems differ across the intersections of these characteristics (e.g., white female). This paper reports on a component of the ATTUNE research programme (work package 2) which aims to investigate the impact and mechanisms of youth adversity on depressive and anxiety symptoms in young people by intersectionality profiles.MethodsThe data are from 4 UK adolescent cohorts: HeadStart Cornwall, Oxwell, REACH, and DASH. These cohorts were assembled for adolescents living in distinct geographical locations representing coastal, suburban and urban places in the UK. Youth adversity was assessed using a series of self-report questionnaires and official records. Validated self-report instruments measured depressive and anxiety symptoms. A range of different variables were classified as possible social and cognitive mechanisms.Results and analysisStructural equation modelling (e.g., multiple group models, latent growth models) and multilevel modelling will be used, with adaptation of methods to suit the specific available data, in accord with statistical and epidemiological conventions.DiscussionThe results from this research programme will broaden our understanding of the association between youth adversity and mental health, including new information about intersectionality and related mechanisms in young people in the UK. The findings will inform future research, clinical guidance, and policy to protect and promote the mental health of those most vulnerable to the negative consequences of youth adversity.
Accumulating Sedentary Time and Physical Activity from Childhood to Adolescence and Cardiac Function in Adolescence
Authors: Haapala Eero A. , Leppänen Marja H. , Lee Earric , Savonen Kai , Laukkanen Jari A. , Kähönen Mika , Brage Soren , Lakka Timo A. 
Published: July 2023

ABSTRACTBackgroundLittle is known about the associations of a sedentary lifestyle from childhood with cardiac work and function during adolescence. We studied if cumulative sedentary time and physical activity from childhood to adolescence are associated with cardiac work and function in adolescence.MethodsA total of 153 adolescents aged 15 years at the time of assessment of cardiac work and function participated. We assessed sedentary time and physical activity using a combined movement and heart rate sensor in childhood and adolescence (baseline, 2- and 8-year follow-ups) and cardiac work and function using impedance cardiography in adolescence (8-year follow-up).ResultsCumulative sedentary time from baseline over the follow-up was directly (standardised regression coefficient β=0.245 to 0.246, 95% confidence intervals, CI=0.092 to 0.400) and moderate to vigorous physical activity (β=-0.355 to -0.323, 95% CI=-0.579 to - 0.119), and vigorous physical activity (β=-0.305 to -0.295, 95% CI=-0.524 to -0.083) from baseline over the follow-up was inversely associated with cardiac work at 8-year follow-up. Cumulative vigorous physical activity from baseline to 2- and 8-year follow-up was inversely associated with cardiac work index at 8-year follow-up (β=-0.225 to -0.218, 95% CI=-0.450 to 0.000). However, adiposity and other cardiometabolic risk factors partially explained these associations.ConclusionsHigher levels of sedentary time and lower levels of moderate and/or vigorous-intensity physical activity during childhood were associated with higher cardiac work in adolescence. These findings highlight the importance of obesity prevention and weight management and promotion physically active lifestyle since childhood to prevent abnormalities in cardiac function later in life.CLINICAL PERSPECTIVESedentary lifestyle increases the risk of cardiovascular diseases, but little is known about the role of sedentary time and physical activity in cardiac work and function in youth.We found that adolescents accumulating higher levels of sedentary time and lower levels of physical activity since childhood had higher cardiac workload compared to their more physically active peers. However, these associations were partly explained by adiposity and other cardiometabolic risk factors.These findings highlight the importance of obesity prevention and weight management and promotion physically active lifestyle since childhood to prevent abnormalities in cardiac function later in life.
The machine learning algorithm identified COL7A1 as a diagnostic marker for LUSC and HNSC
Authors: Wang Chenyu , Ma Yongxin , Qi Jiaojiao , Jiang Xianglai 
Published: July 2023

AbstractSquamous cell carcinomas (SCCs) comes from different parts, but there may be similar tumorigenic signaling pathways and metabolism, and different squamous cell carcinoma has a similar mutation landscape and squamous differentiation expression. Studying the expression profile of common SCCs is helpful to find biomarkers with diagnostic and prognostic significance for a variety of squamous cell carcinoma. Lung squamous cell carcinoma (LUSC), head and neck squamous cell carcinoma (HNSC), and ‘squamous cell cancer’ in esophageal carcinoma (ESCA) and cervical squamous cell carcinoma and endocervical adenocarcinoma (CESC) in The Cancer Genome Atlas (TCGA) database were used as training sets. The relevant data sets in the Gene Expression Omnibus (GEO) database were selected as validation sets. Machine learning algorithms were used to screen out factors with high accuracy in the diagnosis of SCCs as core genes, and explore their effects on patient prognosis and immunotherapy. COL7A1 (Collagen Type VII Alpha 1 Chain) has high accuracy in the diagnosis of LUSC and HCSC, whether in the training set (LUSC _ AUC: 0.987; HNSC _ AUC: 0.933) or validation set (LUSC _ AUC: 1.000; HNSC _ AUC: 0.967). Moreover, the expression of COL7A1 was significantly correlated with shorter OS and DSS in HNSC and LUSC patients, and was also significantly negatively correlated with IPS in LUSC patients treated with CTLA4 (-) PD1 (+), CTLA4 (+) PD1 (-) and CTLA4 (+) PD1 (+). COL7A1 has the potential to be used as a diagnostic and prognostic marker for LUSC and HNSC and to predict the efficacy of LUSC immunotherapy.
The epidemiological investigation of hypertension in northern Henan Province
Authors: Lu Xing , Lin Ziyang , Yang Junzheng 
Published: July 2023

AbstractBackground/purposeTo understand the epidemiological status and risk factors of hypertension in northern Henan province, aim to provide a basis for the prevention, diagnosis, and treatment of hypertension in northern Henan Province.MethodsTo collect the clinical data in Xinxiang Central Hospital from 2019-2021, those data were classified and analyzed according to the basic information of investigation subjects including gender, ethnicity, age, smoking, marital status, occupation, education, and potential risk factors including hyperlipidemia, diabetes mellitus, ischemic stroke, coronary heart disease, angina pectoris, myocardial infarction, heart failure, peripheral artery disease, chronic renal insufficiency, gastrointestinal ulcers, chronic obstructive pulmonary disease, cerebral hemorrhage.ResultsA total of 770 clinical data of investigation subjects were collected, there are 621 males and 149 females, accounting for 80.65% and 19.35%, respectively; age range of investigation subjects was 20-97 years old; there were 248 males with hypertension, accounting for 32.21% in the total population, and there were 92 females with hypertension, accounting for 27.06% in the total population; there were 329 people with a history of smoking, and 346 people were never smoking, accounting for 49.10% and 51.64% in the total investigation population, respectively; in this investigation, the proportion of married people is the highest (638/670, 95.22%); the main occupational population was peasant farmer (353/670, 52.69%), followed by (164/670, 24.48%); the number of people with middle school/vocational school education was the highest(338/670, 50.45%), followed by primary school education (249/670, 37.16%).the results of statistical analysis demonstrated that the incidence of hypertension is related to gender (P<0.01), hyperlipidemia (P<0.05), diabetes mellitus (P<0.001) and ischemic stroke (P<0.001), and had no relation to smoking, family history of coronary heart disease, family history of angina pectoris, history of heart failure, history of coronary intervention, history of peripheral arterial disease, chronic renal insufficiency, history of peptic ulcer disease, history of Chronic obstructive pulmonary disease and history of cerebral hemorrhage (P>0.05).ConclusionThere is a certain regional specificity in the epidemiology of hypertension in the northern Henan Province, and the incidence of hypertension was related to gender, age, occupation, hyperlipidemia, diabetes mellitus and ischemic stroke, those evidences may provide clinical significance for the prevention, diagnosis, and treatment of hypertension in northern Henan province.
Cognitive behavioural therapy-based interventions for gastroduodenal disorders of gut-brain interaction: A systematic review
Authors: Law Mikaela , Pickering Isabella , Bartlett Esme , Sebaratnam Gabrielle , Varghese Chris , Gharibans Armen , O’Grady Greg , Andrews Christopher N. , Calder Stefan 
Published: July 2023

AbstractObjectiveCognitive behavioural therapy (CBT) is increasingly used to manage Disorders of Gut-Brain Interaction (DGBIs). This systematic review aimed to review the evidence for the effectiveness of CBT-based interventions for patients with gastroduodenal DGBIs.MethodsMedline, Embase, PubMed, Cochrane Central, and Scopus were searched in July 2022. Studies were included if they investigated the effects of a CBT-based intervention on gastrointestinal symptoms and/or psychological outcomes pre- and post-intervention in patients with gastroduodenal DGBIs. Case studies, studies not in English, and studies with patients under 18 years were excluded. Results were synthesised narratively, and standardised effect sizes were calculated where possible.ResultsNine studies (seven RCTs and two pre/post studies) were identified, with data reported in 10 articles (total N=602). The studies investigated patients with functional dyspepsia (n=7), rumination syndrome (n=1), and supragastric belching (n=1). The studies had heterogeneous interventions, methodologies, and outcomes, precluding meta-analysis, as well as a moderate-high risk of bias and high drop-outs rates. Findings demonstrated decreased gastrointestinal symptoms and improved anxiety, depression, and quality of life, from pre- to post-intervention, with medium to large effect sizes for symptoms and small to large effect sizes for psychological outcomes. Efficacy was maintained at follow-up, up to one year later.ConclusionsThis review suggests promising evidence that CBT effectively improves gastrointestinal symptoms and psychological outcomes in patients with gastroduodenal DGBIs. However, heterogeneity, risk of bias, and lack of statistical reporting were noted, indicating the need for more robust research and standardisation.
Spinal mobilization characteristics: a scoping literature review of biomechanical parameters
Authors: Gorrell Lindsay M , Nyirö Luana , Pasquier Mégane , Pagé Isabelle , Heneghan Nicola R , Schweinhardt Petra , Descarreaux Martin 
Published: July 2023

BackgroundSpinal mobilization (SMob) is often included in the conservative management of spinal pain conditions as a recommended and effective treatment. While some studies quantify the biomechanical (kinetic) parameters of SMob, interpretation of findings is difficult due to poor reporting of methodological details. The aim of this study was to synthesise the literature describing biomechanical parameters of manually applied SMob.MethodsThis study is reported in accordance with the Preferred Reporting Items for Scoping Reviews (PRISMA-ScR) statement. Databases were searched from inception to October 2022: MEDLINE (Ovid), Embase, CINAHL, ICL, PEDro and Cochrane Library. Data were extracted and reported descriptively for the following domains: general study characteristics, number of and characteristics of individuals who delivered/received SMob, region treated, equipment used and biomechanical parameters of SMob.ResultsOf 7,607 records identified, 36 (0.5%) were included in the analysis. Of these, SMob was delivered to the cervical spine in 13 (36.1%), the thoracic spine in 3 (8.3%) and the lumbopelvic spine in 18 (50.0%) studies. In 2 (5.6%) studies, spinal region was not specified. For SMob applied to all spinal regions, biomechanical parameters were: peak force (0-128N); duration (10-120s); frequency (0.1-4.5Hz); and force amplitude (1-102N).ConclusionsThis study reports considerable variability of the biomechanical parameters of SMob. In studies reporting biomechanical parameters, SMob was most frequently delivered to the lumbar and cervical spine of humans and most commonly peak force was reported. Future studies should focus on the detailed reporting of biomechanical parameters to facilitate the investigation of clinical dose-response effects.
Pneumococcal Serotype Prevalence in Southeast Asia: A Systematic Review and Meta-Analysis
Authors: Lister Alex J. J. , Dombay Evelin , Cleary David W. , Clarke Stuart C. 
Published: July 2023

AbstractBackgroundThe prevalence ofStreptococcus pneumoniaeserotypes in the ASEAN region is not well studied despite severe pneumococcal infections being a major cause of death among children in the region. This information is crucial for understanding the epidemiology of the disease and guiding vaccine policies. Our study aimed to provide a thorough analysis of the pneumococcal serotypes in ASEAN prior to vaccine introduction to assist countries in formulating evidence-based vaccine policies.MethodsWe conducted a systematic review and meta-analysis of studies reportingS. pneumoniaeserotypes from carriage, invasive disease (IPD) and non-invasive disease (non-IPD) published up until 31stDecember 2022 using PubMed, PubMed Central, Ovid MEDLINE and Scopus databases, reference lists and search engines. Data prior to the national introduction of conjugate vaccines in the ASEAN region were included. Non-English, animal, case studies, reviews, and studies on antibiotic resistance only were excluded. The quality of studies was examined using the CASP and the JBI’s Critical Appraisal Tools. The frequency of serogroups and serotypes was calculated, and vaccine coverage was estimated by the addition of vaccine serotypes as a fraction of the total number of isolates per age group. This study was registered with PROSPERO CRD42022243994.FindingsA total of 940 studies were identified, and 99 and 84 relevant papers were included in the qualitative and quantitative analyses, respectively. A total of 16,396 isolates were identified, spread across all body sites and ages, with vaccine-covered serotypes 19F (n = 2,061, 12.57% [95%CI: 12.07 – 13.09]), 23F (n = 1,508, 9.20% [95%CI: 8.76 – 9.65]) and 6B (n = 1,160, 7.07% [95%CI: 6.69 – 7.48]) occurring most often. Non-vaccine typese.g., 6AB (n = 617, 3.76% [95%CI: 3.48 – 4.07]), 15BC (n = 35, 2.57% [95%CI: 2.33 – 2.82]) and 34 (n = 260, 1.59% [95%CI: 1.40 – 1.79]) were also frequently observed.InterpretationThe most common serotypes found in IPD, non-IPD, and carriage in ASEAN are covered by currently available conjugate vaccines. This underscores the importance of vaccination and predicts future success in reducing the burden of pneumococcal disease. The data gathered offers important insights into pneumococcal serotype epidemiology across the different countries belonging to ASEAN.
CRISPR-Cas9 knockdown of ESR1 in preoptic GABA-kisspeptin neurons suppresses the preovulatory surge and estrous cycles in female mice
Authors: Clarkson Jenny , Yip Siew Hoong , Porteous Robert , Kauff Alexia , Heather Alison K. , Herbison Allan E. 
Published: July 2023

AbstractEvidence suggests that estradiol-sensing preoptic area GABA neurons are involved in the pre-ovulatory surge mechanism necessary for ovulation. In vivo CRISPR-Cas9 editing was used to achieve a 60-70% knockdown in estrogen receptor alpha (ESR1) expression by GABA neurons located within the region of the rostral periventricular of the third ventricle (RP3V) and medial preoptic nuclei (MPN) in adult female mice. Mice exhibited variable reproductive phenotypes with the only significant finding being those mice with bilateral ESR1 deletion in RP3V GABA neurons that had reduced cFos expression in GnRH neurons at the time of the surge. One sub-population of RP3V GABA neurons expresses kisspeptin. Re-grouping ESR1-edited mice on the basis of their RP3V kisspeptin expression revealed a highly consistent phenotype; mice with a near complete loss of kisspeptin immunoreactivity displayed constant estrus and failed to exhibit surge activation but retained pulsatile LH secretion. These observations demonstrate ESR1-expressing GABA-kisspeptin neurons in the RP3V are essential for the murine preovulatory LH surge mechanism.
PseudoCell: A Multi-Valued Logical Regulatory Network to Investigate Premature Senescence Dynamics and Heterogeneity
Authors: Pierdoná Vinícius , Lavandoski Patrícia , Maurmann Rafael Moura , Borges Guilherme Antônio , Mombach Jose Carlos Merino , Guma Fátima Theresinha Costa Rodrigues , Barbé-Tuana Florencia María 
Published: July 2023

ABSTRACTPremature cellular senescence is a pivotal process in aging and age-related diseases, triggered by various stressors. However, this is not a homogeneous phenotype, but a heterogeneous cellular state composed of multiple senescence programs with different compositions. Therefore, understanding the complex dynamics of senescence programs requires a systemic approach. We introduce PseudoCell, a multi-valued logical regulatory network designed to explore the molecular intricacies of premature senescence. PseudoCell integrates key senescence signaling pathways and molecular mechanisms, offering a versatile platform for investigating diverse premature senescence programs initiated by different stimuli. Validation through simulation of classical senescence programs, including oxidative stress-induced senescence (OSIS) and oncogene-induced senescence (OIS), demonstrates its ability to replicate molecular signatures consistent with empirical data. Additionally, we explore the role of CCL11, a novel senescence-associated molecule, through simulations that reveal potential pathways and mechanisms underlying CCL11-mediated senescence induction. In conclusion, PseudoCell provides a systematic approach to dissecting premature senescence programs and uncovering novel regulatory mechanisms.
A Motion Transformer for Single Particle Tracking in Fluorescence Microscopy Images
Authors: Zhang Yudong , Yang Ge 
Published: July 2023

AbstractSingle particle tracking is an important image analysis technique widely used in biomedical sciences to follow the movement of sub-cellular structures, which typically appear as individual particles in fluorescence microscopy images. In practice, the low signal-to-noise ratio (SNR) of fluorescence microscopy images as well as the high density and complex movement of subcellular structures pose substantial technical challenges for accurate and robust tracking. In this paper, we propose a novel Transformer-based single particle tracking method called Motion Transformer Tracker (MoTT). By using its attention mechanism to learn complex particle behaviors from past and hypothetical future tracklets (i.e., fragments of trajectories), MoTT estimates the matching probabilities between each live/established tracklet and its multiple hypothesis tracklets simultaneously, as well as the existence probability and position of each live tracklet. Global optimization is then used to find the overall best matching for all live tracklets. For those tracklets with high existence probabilities but missing detections due to e.g., low SNRs, MoTT utilizes its estimated particle positions to substitute for the missed detections, a strategy we refer to as relinking in this study. Experiments have confirmed that this strategy substantially alleviates the impact of missed detections and enhances the robustness of our tracking method. Overall, our method substantially outperforms competing state-of-the-art methods on the ISBI Particle Tracking Challenge datasets. It provides a powerful tool for studying the complex spatiotemporal behavior of subcellular structures. The source code is publicly available athttps://github.com/imzhangyd/MoTT.git.