bluuebunny/crossref_metadata_2025_split huggingface dataset:
Structure-guided design and optimization of covalent VHL-targeted sulfonyl fluoride PROTACs
Authors: Shah Rishi R. , De Vita Elena , Conole Daniel , Sathyamurthi Preethi S. , Zhang Xinyue , Fellows Elliot , Fleites Carlos M. , Queisser Markus A. , Harling John D. , Tate Edward W. 
Published: June 2023

AbstractAbstract FigureProteolysis-targeting chimeras (PROTACs) are heterobifunctional molecules that have emerged as a therapeutic modality to induce targeted protein degradation (TPD) by harnessing cellular proteolytic degradation machinery. PROTACs which ligand the E3 ligase in a covalent manner have attracted intense interest, however, covalent PROTACs with a broad protein of interest (POI) scope have proven challenging to discover by design. Here, we report structure-guided design and optimization of Von Hippel-Lindau (VHL) protein-targeted sulfonyl fluorides which covalently bind Ser110 in the HIF1α binding site. We demonstrate that their incorporation in bifunctional degraders induces targeted protein degradation of BRD4 or androgen receptor (AR) without further linker optimization. Our study discloses the first covalent VHL ligands which can be implemented directly in bifunctional degrader design expanding the substrate scope of covalent E3 ligase PROTACs.
Acute targeted induction of gut-microbial metabolism affects host clock genes and nocturnal feeding
Authors: Greter Giorgia , Moresi Claudia , Oswald Stefanie , de Wouters d’Oplinter Alice , Künzli Daria , Cappio Barazzone Elisa , Lan Jiayi , Slack Emma , Arnoldini Markus 
Published: June 2023

SummaryThe gut microbiota and host circadian clock mutually influence each other, and microbiota metabolism has been shown to play a role in regulating host circadian function via secretion of fermentation products. Microbial metabolism is dependent on the availability of nutrients for the microbiota, typically through the host’s food intake, making it challenging to disentangle the effect of host and microbiota metabolism. In this study, we acutely induced gut microbial metabolic activity without inducing host metabolism in mice. We found that increasing microbial metabolism in the gut altered circadian clock gene expression locally. Actuating microbiota metabolism also reduced host food intake beyond the calories provided by the microbiota, suggesting a systemic signaling effect of microbial metabolism on the host.
Novel hydrogen- and iron-oxidizing sheath-producing Zetaproteobacteria thrive at the Fåvne deep-sea hydrothermal vent field
Authors: Hribovšek Petra , Denny Emily Olesin , Dahle Håkon , Mall Achim , Viflot Thomas Øfstegaard , Boonnawa Chanakan , Reeves Eoghan P. , Steen Ida Helene , Stokke Runar 
Published: June 2023

AbstractIron oxidizing Zetaproteobacteria are well-known to colonize deep-sea hydrothermal vent fields around the world where iron-rich fluids are discharged into oxic seawater. How inter-field and intra-field differences in geochemistry influence the diversity of Zetaproteobacteria, however, remains largely unknown. Here, we characterize Zetaproteobacteria phylogenomic diversity, metabolic potential, and morphologies of the iron oxides they form, with a focus on the recently discovered Fåvne vent field. Located along the Mohns ridge in the Arctic, this vent field is a unique study site with vent fluids containing both iron and hydrogen with thick iron microbial mats (Fe mats) covering porously venting high-temperature (227-267 °C) black smoker chimneys. Through genome-resolved metagenomics and microscopy, we demonstrate that the Fe mats at Fåvne are dominated by tubular iron oxide sheaths, likely produced by Zetaproteobacteria of genusGhiorsea. With these structures,Ghiorseamay provide a surface area for members of other abundant taxa such as Campylobacterota, Gammaproteobacteria and Alphaproteobacteria. Furthermore,Ghiorsealikely oxidizes both iron and hydrogen present in the fluids, with severalGhiorseapopulations co-existing in the same niche. Homologues of Zetaproteobacteria Ni,Fe hydrogenases and iron oxidation genecyc2were found in genomes of other community members, suggesting exchange of these genes could have happened in similar environments. Our study provides new insights into Zetaproteobacteria in hydrothermal vents, their diversity, energy metabolism and niche formation.ImportanceKnowledge on microbial iron oxidation is important for understanding the cycling of iron, carbon, nitrogen, nutrients, and metals. The current study yields important insights into the niche sharing, diversification, and Fe(III) oxyhydroxide morphology ofGhiorsea, an iron- and hydrogen oxidizing Zetaproteobacteria representative belonging to ZetaOTU9. The study proposes thatGhiorseaexhibits a more extensive morphology of Fe(III) oxyhydroxide than previously observed. Overall, the results increase our knowledge on potential drivers of Zetaproteobacteria diversity in iron microbial mats and can eventually be used to develop strategies for the cultivation of sheath-forming Zetaproteobacteria.
Body size shapes song in honeyeaters
Authors: Hay Eleanor M. , McGee Matthew D. , White Craig R. , Chown Steven L. 
Published: June 2023

ABSTRACTBirdsongs are among the most distinctive animal signals. Their evolution is thought to be shaped simultaneously by habitat structure and by the constraints of morphology. Habitat structure affects song transmission and detectability, thus influencing song (the acoustic adaptation hypothesis), while body size and beak size and shape necessarily constrain song characteristics (the morphological constraint hypothesis). Yet, support for the acoustic adaptation and morphological constraint hypotheses remains equivocal, and their simultaneous examination is infrequent. Using a phenotypically diverse Australasian bird clade, the honeyeaters (Aves: Meliphagidae), we compile a dataset consisting of song, environmental, and morphological variables for 163 species and jointly examine predictions of these two hypotheses. Overall, we find that body size constrains song frequency and pace in honeyeaters. Although habitat type and environmental temperature influence aspects of song, that influence is indirect, likely via effects of environmental variation on body size, with some evidence that elevation constrains the evolution of song peak frequency. Our results demonstrate that morphology has an overwhelming influence on birdsong, in support of the morphological constraint hypothesis, with the environment playing a secondary role generally via body size rather than habitat structure. These results suggest that changing body size, a consequence of both global effects such as climate change, and local effects such as habitat transformation, will substantially influence the nature of birdsong.
Kir2.1 modulation in macrophages sensitises dorsal root ganglion neurons through TNF secretion after nerve injury
Authors: Konnova Elena A. , Deftu Alexandru-Florian , Chu Sin Chung Paul , Kirschmann Guylène , Decosterd Isabelle , Suter Marc R. 
Published: June 2023

AbstractMacrophages and satellite glial cells are found between injured and uninjured neurons in the lumbar dorsal root ganglia (DRG). We explored the mechanism of neuro-immune and neuron–glia crosstalk leading to hyperexcitability of DRG neurons. After spared nerve injury (SNI), CX3CR1+resident macrophages became activated, proliferated and increased inward-rectifying potassium channel Kir2.1 currents. Conditioned medium (CM) by macrophages, obtained from DRG of SNI mice, sensitised small DRG neurons from naïve mice. However, treatment with CM from GFAP+glial cells did not affect neuronal excitability. When subjected to this macrophage-derived CM, DRG neurons had increased spontaneous activity, current-evoked responses and voltage-gated NaV1.7 and NaV1.8 currents. Silencing Kir2.1 in macrophages after SNI prevented the induction of neuronal hyperexcitability from their CM. Blocking vesicular exocytosis or soluble tumour necrosis factor (TNF) in CM or interfering with the downstream intracellular p38 pathway in neurons, also prevented neuronal hyperexcitability. Blocking protein trafficking in neurons reduced the effect of CM, suggesting that the hyperexcitable state resulted from changes in NaVchannel trafficking. These results suggest that DRG macrophages, primed by peripheral nerve injury, contribute to neuron–glia crosstalk, NaVchannel dysregulation and neuronal hyperexcitability implicated in the development of neuropathic pain.Graphical abstract
The impact of industrial Internet of things on sustainable performance: the indirect effect of supply chain visibility
Authors: Al-Khatib Ayman Wael 
Published: June 2023

PurposeSustainable management has become a strategic and popular requirement for stakeholders, as the ability to create competitive advantages in the current dynamic business environment and the pressures of digital transformation need to be increased attention in achieving the highest level of sustainable performance. The main objective of this paper is to explore the direct effects of the industrial Internet of things and supply chain visibility on sustainable performance (economic performance, environmental performance and social performance), in addition to evaluating the indirect effects that supply chain visibility plays in these relationships.Design/methodology/approachTo achieve the purposes and objectives of this work, a conceptual model was developed by referring to the current works in the literature, and the theoretical model was developed, and then data were collected through the questionnaire and by the snowball sampling method, as 380 questionnaires were used. In the data analysis, the multivariate analysis technique was used via Covariance-based Structural Equation Modeling (CB-SEM), as hypothesized relationships were evaluated through confirmatory factor analysis (CFA), and then the hypotheses were tested through a structural model.FindingsThe results reached the acceptance of all hypotheses, and this means that all hypothesized relationships were positive, as the impact of the industrial Internet of things was positive on each of the supply chain visibility and economic performance, environmental performance and social performance, and also the supply chain visibility mediated the relationship between each of the industrial Internet of things and economic performance, environmental performance and social performance.Originality/valueThe current paper reported a range of theoretical and practical contributions, as this study is the first to examine these hypothesized relationships together in this way and thus helped this study to develop new perceptions about Industry 4.0 technologies and their potential impacts on supply chain and sustainable performance. Also, this study had a variety of management recommendations that allow managers of pharmaceutical manufacturing companies operating in Jordan to develop their technological capabilities and use them to enhance sustainable performance.
A hybrid enrichment strategy for the efficient construction of reduced order models by the proper generalized decomposition
Authors: Müller Fabian , Baumanns Paul , Hameyer Kay 
Published: June 2023

Purpose
The calculation of electromagnetic fields can involve many degrees of freedom (DOFs) to achieve accurate results. The DOFs are directly related to the computational effort of the simulation. The effort is decreased by using the proper generalized decomposition (PGD) and proper orthogonalized decomposition (POD). The purpose of this study is to combine the advantages of both methods. Therefore, a hybrid enrichment strategy is proposed and applied to different electromagnetic formulations.

Design/methodology/approach
The POD is an a-priori method, which exploits the solution space by decomposing reference solutions of the field problem. The disadvantage of this method is given by the unknown number of solutions necessary to reconstruct an accurate field representation. The PGD is an a-priori approach, which does not rely on reference solutions, but require much more computational effort than the POD. A hybrid enrichment strategy is proposed, based on building a small POD model and using it as a starting point of the PGD enrichment process.

Findings
The hybrid enrichment process is able to accurately approximate the reference system with a smaller computational effort compared to POD and PGD models. The hybrid enrichment process can be combined with the magneto-dynamic T-Ω formulation and the magnetic vector potential formulation to solve eddy current or non-linear problems.

Originality/value
The PGD enrichment process is improved by exploiting a POD. A linear eddy current problem and a non-linear electrical machine simulation are analyzed in terms of accuracy and computational effort. Further the PGD-AV formulation is derived and compared to the PGD-T-Ω reduced order model.
Utilization of electronic resources: an analysis of awareness and perception of users of Kashmir University
Authors: Wagay Javaid Ahmad , Dutta Saurabh 
Published: June 2023

Purpose
This paper focuses on the use of e-resources by research scholars and post-graduate students at Kashmir University. The main aim is to determine the use of e-resources, users skills in handling e-resources and the purpose of their use. Further, this paper aims to highlight the problems faced by research scholars and post-graduate students in accessing e-resources.

Design/methodology/approach
The survey was conducted through a structured questionnaire circulated among 250 research scholars, both (M.Phil./Ph.D.) and post-graduate students, from different departments of Kashmir University, and the response rate was 80%. Random sample method was used for the selection of respondents and interacting with them. The responses received from the research scholars to 14 questions are presented in the form of tables.

Findings
Major findings of the study reveal that the majority of the students reported using electronic journal resources for various purposes including working on assignments, research proposal writing, literature review writing, research report writing, current awareness and leisurely exploration of ongoing scientific debates through peer-reviewed papers. The challenges encountered in the use of electronic journal resources include a power outage, inadequate bandwidth, slow download speed, inability to access the resources from home, lack of training, lack of awareness, limited access to computers and difficulty in searching. The paper concludes that electronic resources have become an integral part of the information needs of research scholars’ post-graduate students at Kashmir University. Further, it finds that e-resources can be good substitutes for conventional resources, if the access is fast, and more computer terminals are installed to provide fast access to e-resources. Finally, recommendations for improving the use of electronic journal resources are provided.

Practical implications
The paper restrains the study exclusively to use of e-resources by the research scholars and post-graduate students of Kashmir University.

Originality/value
The paper highlights the use of e-resources by research scholars and post-graduate students of Kashmir University and makes some constructive suggestions for the improvement of electronic resources and services. This is the first time an effort has been made to assess the use of electronic resources by post-graduate students at Kashmir University. The study could be used to assess the post-graduate students’ needs for electronic resources at the Kashmir University and other users in higher learning institutions.
The role of renewable energy on life expectancy: evidence from method of moments quantile regression based on G-7 countries data
Authors: Karimi Alavijeh Nooshin , Ahmadi Shadmehri Mohammad Taher , Dehdar Fatemeh , Zangoei Samane , Nazeer Nazia 
Published: June 2023

Purpose
While science has researched the impact of air pollution on human health, the economic dimension of it has been less researched so far. Renewable energy consumption is an important factor in determining the level of life expectancy and reducing health expenditure. Thus, this study aims to investigate the impact of renewable energy, carbon emissions, health expenditure and urbanization on life expectancy in G-7 countries over the period of 2000–2019.

Design/methodology/approach
This study has adopted a novel Method of Moments Quantile Regression (MMQR). Furthermore, as a robustness check for MMQR, the fully modified ordinary least square, dynamic ordinary least squares and fixed effect ordinary least square estimators have been used.

Findings
The results indicated that renewable energy consumption, health expenditure and urbanization lead to an increase in life expectancy across all quantiles (5th to 95th), whereas higher carbon dioxide emissions reduce life expectancy at birth across all the quantiles (5th to 95th).

Practical implications
The empirical findings conclude that governments should recognize their potential in renewable energy sources and devise policies such as tax-related regulations, or relevant incentives to encourage further investments in this field.

Originality/value
This paper in comparison to the other research studies used MMQR to investigate the impact of factors affecting life expectancy. Also, to the best of the authors’ knowledge, so far no study has investigated the impact of renewable energy on life expectancy in G-7 countries.
The role of financial inclusion in moderating the incidence of entrepreneurship on energy poverty in Ghana
Authors: Asongu Simplice , Odhiambo Nicholas M. 
Published: June 2023

Purpose
This study aims to assess the role of financial inclusion (FI) in moderating the incidence of entrepreneurship on energy poverty in Ghana.

Design/methodology/approach
The assessment is made by using pooled data and two-stage least squares. The exposition builds from the 7th (GLSS7) and 6th (GLSS6) rounds focusing on the Ghana Living Standards Survey (GSS, 2014, 2019) that is collected by the Ghana Statistical Service (GSS) from 10 principal regions in the country.

Findings
The findings show that entrepreneurship has an unconditional positive incidence on energy poverty while the interactive incidence between entrepreneurship and FI on energy poverty is negative. The corresponding FI policy thresholds that should be exceeded in order for FI to effectively moderate entrepreneurship for negative outcomes in energy poverty are between 0.154 and 0.280 index for the full sample; 0.187 index for the rural subsample; 0.200 and 0.333 index for the male sample. Thresholds are not computed for the rural and female subsamples because at least one estimated coefficient that is needed for the computation of such thresholds is not significant. Policy implications are discussed.

Originality/value
This study has complemented the existing literature by assessing how FI can be used to influence the nexus between entrepreneurship and poverty in Ghana.
Investigating the level of effectiveness of the anti-fraud technologies employed by the South African banking industry for cyberfraud mitigation
Authors: Akinbowale Oluwatoyin Esther , Klingelhöfer Heinz Eckart , Zerihun Mulatu Fekadu 
Published: June 2023

Purpose
The purpose of this study is to examine the level of effectiveness of the anti-fraud technologies employed by the South African banking industry for cyberfraud mitigation.

Design/methodology/approach
This research employed a qualitative research design involving a purposive sampling method. Primary data was collected from the key organisational staff across the 17 licensed commercial banks in South Africa via the use of structured questionnaires. In particular, these were experts involved in combating fraud and taking managerial decisions regarding the use of anti-fraud technologies for cyberfraud mitigation. Non-parametric statistical analyses were carried out from the responses obtained.

Findings
The results obtained indicated that the combination of internal and external anti-fraud technologies such as filtering software, firewalls, encryption, continuous auditing, discovery sampling, virus protection, financial ratios, digital analysis and data mining may have a positive effect on cyberfraud mitigation. These technologies are employed mostly to ensure effective internal control systems capable of minimising cyberfraud. In addition, the anti-fraud technologies employed in the South African banking industry may also be effective in the mitigation of cyberfraud, although significant cases of cyberattacks were reported by the respondents.

Practical implications
The study recommends investment in more digital and emerging technologies and the development of human capacities to effectively deploy them in the combat against cybercrime.

Originality/value
The novelty of this study lies in the identification of the type of anti-fraud technologies/software employed by the South African banking industry and their level of effectiveness or success rate.
The moderating effect of adaptability on the relationship between cognitive social capital and innovation capacity
Authors: Zirena-Bejarano Patricia Pilar , Tancayllo Yana Graci , Caryt Málaga Andrea Karina 
Published: June 2023

Purpose
The purpose of this study is to analyze the moderating effect of adaptability on the relationship between cognitive social capital and innovation capacity in companies in the tourism sector. The study aims to contribute to a better understanding of the direct and indirect linkage generated between these variables.

Design/methodology/approach
The research was designed under the quantitative approach, and an empirical study was carried out by applying a questionnaire to 300 companies located in Arequipa, Perú. Data processing was conducted using partial least squares structural equation modeling using Smart PLS software.

Findings
The results demonstrate the existence of a positive and significant direct relationship between cognitive social capital and innovation capacity. Furthermore, it was found that adaptability moderates this relationship, improving the results. The study shows that shared goals and culture facilitate the transfer of knowledge, which allows the exploration of new opportunities, and that the accommodation of business resources enables companies to respond quickly to new market requirements, promoting the capacity for innovation.

Practical implications
Organizations operate in an environment of intense competition. Therefore, developing the capacity for innovation is essential to stay competitive. Managers must strengthen cognitive relationships by sharing objectives and culture with their contacts, in addition to developing the ability to respond to market changes. This will stimulate the creation of new products and services.

Originality/value
This document adds value to the literature on social capital and innovation, demonstrating the importance of adaptability and its moderating effect on the relationship between cognitive social capital and innovation capacity. This has not been explored before. The research shows how these three variables act synergistically in a model, improving results, which is essential to achieve the competitiveness of companies in the tourism sector of an emerging country.