bluuebunny/crossref_metadata_2025_split huggingface dataset:
Case Report: A Case Report and Literature Analysis on Intestinal Tuberculosis Intestinal Perforation Complicated by Umbilical Intestinal Fistula and Bladder Ileal Fistula
Authors: Chen Tianyan , Liu Guobin , Kang Quan , Song Xiaofeng , Chen Bolin 
Published: February 2023

Abstract
Objectives and design: This article is to make pediatric surgeons fully aware of the atypical manifestations caused by intestinal tuberculosis, as well as the principles and methods of diagnosis and treatment reported in this case and related literature.
Materials and methods: Description of a case report.
Results: We report a rare case of umbilical fistula and bladder ileal fistula caused by intestinal perforation secondary to intestinal tuberculosis，and analysis and summary the literature on the diagnosis and treatment of intestinal TB.
Conclusions: This is a rare case of umbilical fistula and bladder ileal fistula caused by intestinal perforation secondary to intestinal tuberculosis. The purpose of this article is to make the surgical community aware of this atypical presentation of intestinal tuberculosis so that they can be prepared if they encounter this condition in the future.
The association between fear of missing out and mobile phone addiction: A meta-analysis
Authors: Zhang Yali , Zhang Weina 
Published: February 2023

Abstract
Background
Numerous studies have explored the association between fear of missing out and mobile phone addiction, but the results were inconsistent. This study aims at exploring the relationship between fear of missing out and mobile phone addiction, as well as the reasons for the different results of previous studies.
Methods
In order to achieve our goals, a meta-analysis of 42 studies (43 effect sizes) was conducted with a random-effects model in this research.
Results
The main effect analysis revealed a high positive correlation between fear of missing out and mobile phone addiction (r = 0.47, 95%CI=[0.44, 0.50]). Furthermore, the measurements applied to quantify the variables moderated the strength of the association between fear of missing out and mobile phone addiction, whereas age, gender, year of publication and cultural background didn't.
Conclusions
The results indicated that fear of missing out was closely related to mobile phone addiction, which complied with the Theory of Compensatory Internet Use. In the future, experimental and longitudinal studies are needed to explore the dynamic relationship between fear of missing out and mobile phone addiction.
The belowground of the resurrection plant Myrothamnus flabellifolia: Exploring the desiccation-tolerant root-associated microbiome
Authors: Tebele Shandry M. , Marks Rose A. , Farrant Jill Margaret 
Published: February 2023

Abstract
Aims and Background
The resurrection plant Myrothamnus flabellifolia tolerates complete desiccation and is a great model for studying how plants cope with extreme drought. The response to desiccation in M. flabellifolia leaves has been studied extensively, but little is known about how the associated microbiome contributes to desiccation tolerance. Root-associated microbes play a major role in stress tolerance, and are an attractive target for enhancing drought tolerance in staple crops. However, how these dynamics play out under the most extreme water limitation remains underexplored.
Methods
The high-throughput amplicon metagenomic technique was used to investigated the bacterial and fungal communities in the bulk soil, rhizosphere, and endosphere of the M. flabellifolia.
Results
The bacterial phyla that were most abundant across all compartments were Acidobacteriota, Actinobacteria, Chloroflexi, Planctomycetes andProteobacteria, while fungal phyla were Ascomycotaand Basidiomycota. Although the bulk soil hosted multiple beneficial root-associated microbes, the rhizosphere compartment showed the highest functional diversity of bacteria and fungi. In contrast, the endosphere exhibited a low abundance and diversity of microbes. These findings suggest that M. flabellifolia recruits soil microbes from bulk to rhizosphere, many of which may confer extreme drought tolerance and promote plant growth.
Conclusion
The diverse rhizosphere microbiome is filtered to result in a highly selective endosphere microbiome. We find that compartment niches act as the major driver of microbial diversity, but that soil physicochemical factors also influence the microbial composition. These results suggest that the root-associated microbiome of M. flabellifolia is highly structured and may aid in plant function.
Predicting response and toxicity to immune checkpoint inhibitors in lung cancer using antibodies to frameshift neoantigens
Authors: Shen Luhui , Brown Justin R , Johnston Stephen Albert , Altan Mehmet , Sykes Kathryn Frances 
Published: February 2023

Abstract

Purpose:To evaluate a new class of blood-based biomarkers, anti-frameshift peptide antibodies, for predicting both tumor responses and adverse immune events to immune checkpoint inhibitor (ICI) therapies in advanced lung cancer patients.Experimental Design:Serum samples were obtained from 74 lung cancer patients prior to palliative PD-(L)1 therapies with subsequently recorded tumor responses and immune adverse events (irAEs). Pretreatment samples were assayed on microarrays of frameshift peptides (FSPs), representing ~375,000 variant peptides that tumor cells can be informatically predicted to produce from translated mRNA processing errors. Serum-antibodies recognizing these ligands were measured. Differential binding profiles of best-response and adverse-event contrasts were analyzed. Binding activities shared among an outcome group but distinct relative to the other were identified. Antibody bound FSPs were used in iterative resampling analyses to develop predictive models of clinical response and immune toxicity.Results:Lung cancer serum samples were classified based on predictive models of ICI treatment outcomes. Disease progression was predicted with 100% accuracy pretreatment in the full cohort of all response categories, although half of the samples did not show the minimum number of differential binding events to classify. This model included samples from patients that i) would show either responsive or stable outcomes, ii) would be administered either single or combination therapies and iii) were diagnosed with different lung cancer subtypes. Removing the stable disease group or those who would receive combination chemotherapy increased the proportion of samples classified. On the other hand, removing small cell lung cancer from the non-small cell cancer samples had little impact. Informatic analyses showed that several of the FSPs in the all-response model mapped to translations of variant mRNAs from the same genes. In the predictive model for treatment toxicities, binding to irAE-associated FSPs provided 88% prediction accuracy pretreatment, with no indeterminates. Several of the classifying FSPs displayed sequence similarity to self-proteins.Conclusions:Anti-FSP antibodies may serve as biomarkers for predicting ICI outcomes when tested against ligands corresponding to mRNA-error derived FSPs. Model performances suggest this approach might provide a single test to predict treatment response to ICI and identify patients at high risk for immunotherapy toxicities.
Dietary Exposure Risk Assessment of Pyrethroids in Fruits and Vegetables: A National Scale Investigation
Authors: Li Qianqian , Li Bei , Chen Dawei , Zhang Rong , Liu Shuyan , Yang Shupeng , Li Yi , Li Jianxun 
Published: February 2023

Abstract
The pyrethroids (PYRs) were extensively used to increase agriculture outputs. However, the cumulative exposures of PYRs would bring about potential risks through food intake. It is in urgent requirement to explore the cumulative exposures on the fruits and vegetables. In this study, a total of 1720 samples incorporating eight primary fruits and vegetables collected around China were investigated to assess the health risk for adults and children from eight PYRs. The relative potency factor (RPF) method was employed to reveal both chronic and acute cumulative exposure. As a result, the hazard index (HI) were 0.004%~0.200% and 11.85%~99.19% for chronic and acute cumulative dietary exposure, respectively. The national wide investigation indicated the cumulative assessments were not hazardous. Besides, the acute intake of pear, grape, and lettuce should be paid on more attention, particularly. This study provides compelling evidence to develop relative policy and regulation to improve the food quality and safety.
The mannitol-modified emodin nano drug restored the intestinal barrier function and alleviated inflammation in a mouse model of DSS-induced colitis
Authors: Xu Yinyue , Zhu Min , Wu Jiang , Luo Longbiao , Dong Sijing , Zhang Menggai , Liu Xue , Wang Ke , Wang Yitao , Jing Wanghui , Wang Lin , Wang Sicen 
Published: February 2023

Abstract
Background
Ulcerative colitis (UC) is an inflammatory disease of the colon that is characterized by mucosal ulcers. Given its increasing prevalence worldwide, it is imperative to develop safe and effective drugs for treating UC. Emodin, a natural anthraquinone derivative presenting in various medicinal herbs, has demonstrated therapeutic effects against colitis. However, low bioavailability due to poor water solubility limits its clinical applications.
Methods
Emodin-borate nanoparticles (EmB) were synthesized to improve drug solubility, and modified with oligomeric mannitol into microgels (EmB-MO) for targeted delivery to intestinal cells expressing mannose receptors. Colitis was induced in a mouse model using dextran sulphate sodium (DSS), and the different drug formulations were administered to the mice via drinking water. The levels of inflammation-related factors in the colon tissues and fecal matter were measured by enzyme linked immunosorbent assay (ELISA). Intestinal permeability was evaluated using fluorescein isothiocyanate dextran. HE staining, in vivo imaging, real-time PCR and western blotting were performed to assess intestinal barrier dysfunction.
Results
Both EmB and EmB-MO markedly alleviated the symptoms of colitis, including body weight loss, stool inconsistency and bloody stools, and restored the levels of pro- and anti-inflammatory cytokines. However, the therapeutic effects of EmB-MO on the macroscopic and immunological indices were stronger compared to that of EmB, and similar to that of 5-aminosalicylicacid. Furthermore, EmB-MO selectively accumulated in the inflamed colon epithelium, and restored the levels of the gut barrier proteins ZO-1 and occludin.
Conclusions
Encapsulation of EmB-MO significantly improved water solubility, which translated to greater therapeutic effects on the immune balance and gut barrier function in mice with DSS-induced colitis. Our findings provide novel insights into developing emodin-derived drugs for the management of UC.
RankDMG: a differentially methylated gene analysis method for three special scenarios
Authors: Fan Qi , Zheng Linyong , Chen Haifeng , He Jun , Yang Fenglong , Shen Xiaopei , Yan Haidan 
Published: February 2023

Abstract
Background
Common methods of identification of differentially methylated genes (DMGs) mainly detect differences between case and control groups, which cannot tell whether a gene is differentially methylated in a specific disease sample (first scenario), and are not applicable for the study with no normal control (one-phenotype, second scenario). Also, these methods have low detection capacity at the control-limited (third) scenario.
Results
we developed a method, termed RankDMG, to analyze DNA methylation data in the three special scenarios. For the individualized DMG analysis, RankDMG showed remarkable performances in simulated and real data, independent of measured platforms. Using DMGs detected by common methods as ‘gold standard’, the DMGs identified by RankDMG using only one-phenotype data were comparable to those detected by common methods using case-control samples. Moreover, even when the number of disease samples reduced to five, RankDMG could also identify disease-related DMGs for control-limited data.
Conclusion
RankDMG provides a novel tool to dissect the inter-individual heterogeneity of tumor at epigenetic level, and it could analyze the one-phenotype and control-limited methylation data. RankDMG is provided as an open source tool via https://github.com/FunMoy/RankDMG.
Screw fixation without fusion for low lumbar Chance fracture accompanied by spinal epidural hematoma in patient with ankylosing spondylitis
Authors: Kim Dae Kyun , Kim Seok Won 
Published: February 2023

Abstract
Ankylosing spondylitis (AS) is a chronic inflammatory disease involving the sacroiliac joint and axial spine. AS may render the ankylosed spine prone to trauma and cause an increased frequency of associated epidural hematomas in spine fractures. Herein, we report a rare case of L5 chance fracture and epidural hematoma in a 27-year-old female patient with AS. She was treated surgically but without bone fusion or decompressive laminectomy due to the neurologically intact status despite significant neural compression by the spinal epidural hematoma (SEH). We believe that conservative treatment with close observation of neurological status may be effective in SEH presenting with mild neurological symptoms despite significant neural compression. It was possible to preserve motion segments by minimally invasive percutaneous screw fixation instead of conventional long-level fixation and bone fusion in low lumbar chance fractures accompanying SEH in patients with AS.
A simple and available measurement of onco-exosome dsDNA to protein ratio as a potential tumor marker
Authors: Sun Yifan , Li Miao , Zhang Xiaoshan , Xu Dongjie , Wu Jie , Gu Xinrui , Khan Adeel , Shen Han , Li Zhiyang 
Published: February 2023

Abstract
Background: Exosomes have great potential as new biomarkers in liquid biopsy. However, due to the limitations of exosome extraction and component analysis procedures, further clinical applications of exosomes are hampered. Carcinoembryonic antigen (CEA) is a commonly used broad-spectrum tumor marker that is strongly expressed in a variety of malignancies.
Results: In this study, CEA+ exosomes were directly separated from serum using immunomagnetic beads, and the nucleic acid to protein ultraviolet absorption ratio (NPr) of CEA+ exosomes was determined. It was found that the NPr of CEA+ exosomes in tumor group was higher than that of healthy group. We further analyzed the exosome-derived nucleic acid components using fluorescent staining and found that the concentration ratio of double-stranded DNA to protein (dsDPr) in CEA+ exosomes was also significantly different between the two groups, with a sensitivity of 100% and a specificity of 41.67% for the diagnosis of pan-cancer. The AUC of dsDPr combined with NPr was 0.87 and the ACU of dsDPr combined with CA242 could reach 0.94, showing good diagnostic performance for pan-cancer.
Conclusions: This study demonstrates that the dsDPr of CEA+ exosomes can effectively distinguish exosomes derived from tumor patients and healthy individuals, which can be employed as a simple and cost-effective non-invasive screening technology to assist tumor diagnosis.
Fungi associated with stem collar necroses of Fraxinus excelsior affected by ash dieback
Authors: Peters Sandra , Fuchs Sebastian , Bien Steffen , Bußkamp Johanna , Langer Gitta Jutta , Langer Ewald Johannes 
Published: February 2023

Abstract
In recent decades the vitality and physical stability of European ash trees in Germany have been reduced by European ash dieback, especially when associated with stem collar necroses and rots. This study was carried out to investigate the composition of the fungal communities associated with stem collar necroses. Filamentous fungi were isolated from 58 ash trees out of nine forest stands in northern, eastern, and central Germany. Obtained isolates were identified to a genus or species level by means of morphological and molecular analyses. In total 162 morphotypes including endophytic, saprotrophic and pathogenic fungi were isolated. For 33 species found no prior reports from Fraxinus excelsior were recognised, including Cryptostroma corticale and Diplodia sapinea. None of the identified species were found at all studied sites, though Diplodia fraxini was the most common fungi with regard to frequency within all isolates, occurring at seven sample sites. This species is followed by Hymenoscyphus fraxineus, Armillaria spp., Neonectria punicea, Diaporthe cf. eres, Fusarium cf. lateritium, and Paracucurbitaria sp. in order of frequency within all isolates. The aforementioned species are characterised and analysed in respect to their occurrence in stem collar necroses and at sample sites. The influence of site conditions on the fungal composition was described for five intensively sampled sites with a minimum of five studied trees (Schwansee, Rhüden, Berggießhübel, Satrup, and Schlangen). The sampling site of Schlangen was further subdivided into four subplots with different positions in the terrain. In the remaining four extensive sample sites either one or two trees, respectively, were sampled and analysed (Oranienbaumer Heide, Woltershausen, Wolfenbüttel, and Neuhege). Over all sample sites fungal communities of symptomatic stem tissue are similar concerning the most frequent fungi, but vary greatly according to singularly isolated fungi.
Clinical characteristics and outcomes of women with adenomyosis pain during pregnancy: a retrospective study
Authors: SAYAMA Seisuke , IRIYAMA Takayuki , TAKEIRI Yotaro , HASHIMOTO Ayako , TOSHIMITSU Masatake , ICHINOSE Mari , SEYAMA Takahiro , SONE Kenbun , KUMASAWA Keiichi , NAGAMATSU Takeshi , KOGA Kaori , OSUGA Yutaka 
Published: February 2023

Abstract
Background
Adenomyosis is known to be associated with unfavorable perinatal outcomes, but the patient population among women with adenomyosis who is at high risk for adverse perinatal outcomes remains unclear. Recent case reports show that some women with adenomyosis experience pain at the adenomyosis lesion during pregnancy and have detrimental perinatal outcomes. However, the prevalence of pain onset in women with adenomyosis has not been studied, nor has its influence on perinatal outcomes. This study aimed to clarify the clinical characteristics of pain developing in adenomyosis lesions during pregnancy and the perinatal outcomes associated with this phenomenon.
Methods
This was a single-center retrospective analysis of a cohort of women with adenomyosis who delivered between 2011 and 2021. The incidence of pain onset at adenomyosis lesions among women with adenomyosis during pregnancy was analyzed retrospectively from medical records. Pain during pregnancy was defined as persistent pain at the adenomyosis site with administration of analgesics for pain relief, and its association with perinatal outcomes was analyzed.
Results
Among 91 singleton pregnancies with adenomyosis, 12 pregnancies (13.2%) presented with pain at the adenomyosis site during pregnancy. In total, 5 of the 12 pregnancies (41.7%) developed preeclampsia, which resulted in preterm delivery, and only 3 of the 12 pregnancies (25.0%) achieved term delivery. The incidence of preeclampsia and preterm delivery was higher in those who experienced pain than in those who did not (41.7% vs. 13.9%; p < 0.05, and 66.7% vs. 31.7%; p < 0.05, respectively). Among the women who had pain during pregnancy, the maximum C-reactive protein level was significantly higher in women who developed preeclampsia than in those who did not (5.45 vs. 0.12 mg/dL, p < 0.05).
Conclusion
Our study revealed that adenomyosis can cause pain in over one of eight pregnancies with adenomyosis, which may be associated with the increased incidence of preeclampsia resulting in preterm delivery. Women who present with pain at the adenomyosis lesion, especially those with high C-reactive protein levels, may be at a high risk for the future development of preeclampsia and consequent preterm delivery.
topr: an R package for viewing and annotating genetic association results
Authors: Juliusdottir Thorhildur 
Published: February 2023

Abstract
Background
The successful identification of genetic loci for complex traits in genome-wide association studies (GWAS) has resulted in thousands of GWAS summary statistics becoming publicly available for hundreds of complex traits from multiple cohorts and studies. Visualisation is an important aid for interpreting, comparing, validating, and obtaining an overview of large amounts of data. However, the current software is limited in its ability and flexibility to annotate and simultaneously display multiple GWAS results which is useful when interpreting and comparing association results. Therefore, I created the topr R package to facilitate visualisation, annotation, and comparisons of single or multiple GWAS results. It contains functions tailored for viewing and analysing GWAS results.
Results
topr provides a fast and elegant visual display of association results, along with the annotation of association peaks with their nearest gene. Association results from multiple analyses can be viewed simultaneously over the entire genome or in a more detailed regional view along with gene information. Users can perform the essential steps of visually exploring and annotating association results and generating elegant publication-ready plots.
Conclusions.
topr is developed as a package for the R statistical computing environment, released under the GNU General Public License, and is freely available on the Comprehensive R Archive Network (http://cran.r-project.org/package=topr). The source code is available at GitHub (https://github.com/GenuityScience/topr). topr provides several advantages and advances over the current alternatives, particularly in its gene annotation functionality and customisable display of single- or multiple-association results. With topr, I provide a flexible tool with multiple features to aid in the analysis and evaluation of GWAS association results.