bluuebunny/crossref_metadata_2025_split huggingface dataset:
Comparison of Sequencing Methods for Obtaining a Whole Genome of Pea Necrotic Yellow Dwarf Virus from U.K. Peas
Authors: Fowkes A. R. , Duffy S. , McGreig S. , Vazquez-Iglesias I. , Alraiss K. , Barrett B. , Conyers C. , Webster G. , Ward R. , Chisnall K. , Mynett M. , Adams I. P. , Macarthur R. , Howard B. , Fox A. 
Published: May 2023

During a project investigating pea viruses in the United Kingdom and developing a workflow for high-throughput sequencing in crop surveillance, pea necrotic yellow dwarf virus (PNYDV) was identified at one site, along with pea enation mosaic virus-1, pea enation mosaic virus-2, pea enation mosaic virus satellite RNA, pea seedborne mosaic virus, and turnip yellows virus. Following an initial finding of small PNYDV contigs by an RNA sequencing protocol on MiSeq (Illumina), confirmation and prevalence testing by PCR and Sanger sequencing determined the average PNYDV prevalence to be 3%. This is the first report of PNYDV, and a nanovirus, in the United Kingdom. Initial characterization of the octopartite genome by PCR revealed that component DNA N had a truncated sequence that differed from those previously reported. This led to confirmation testing using rolling circle amplification (RCA) into MiSeq and then MinION (Oxford Nanopore). Sequences were obtained by both RCA-MiSeq and RCA-MinION, with MinION producing more complete genomes than RCA-MiSeq. Data obtained from sequencing were used to provide an initial estimation of the genomic formula of PNYDV. Previous work has looked into comparing each method; however, this study shows how these methods can be used together. The truncated sequence for DNA N was also found by RCA-MiSeq and RCA-MinION and confirmed by specific primers, but further work is required to understand the impact of this truncation. [Formula: see text] Copyright © 2023 The Author(s). This is an open access article distributed under the CC BY-NC-ND 4.0 International license .
Innovations in Learning
Authors: DeBrew Jacqueline , Lindsey Karen 
Published: May 2023

Abstract
Because of a dearth of available resources, faculty faced with creating a Foundations of Nursing course aligned with the revised American Association of Colleges of Nursing Essentials for a new accelerated baccalaureate program struggled to design an innovative way to integrate concepts that demonstrate the role of the professional nurse. With the help of a colleague from the Communications Department, an innovative assignment was developed that engaged students throughout the semester. The assignment created a foundation for students’ practice as professional nurses.
Costs of Surgery in Adult Spinal Deformity
Authors: Joujon-Roche Rachel , Dave Pooja , Tretiakov Peter , Mcfarland Kimberly , Mir Jamshaid , Williamson Tyler K. , Imbo Bailey , Krol Oscar , Lebovic Jordan , Schoenfeld Andrew J , Vira Shaleen , Lafage Renaud , Lafage Virginie , Passias Peter G. 
Published: May 2023

Study Design.
Retrospective

Objective.
To assess impact of surgical costs on patient reported outcomes in ASD.

Summary of Background Data.
With increased focus on delivering cost effective healthcare, interventions with high resource utilization, such as adult spinal deformity (ASD) surgery, have received greater scrutiny.

Methods.
ASD patients≥18 years with BL and 2Y data were included. Surgical costs were calculated using 2021 average Medicare reimbursement by CPT code. Costs of complications and reoperations were intentionally excluded. Patients were ranked into tertiles by surgical cost: highest surgical costs (HC) and lowest surgical costs (LC). They were propensity score matched (PSM) to account for differences in baseline age and deformity. Bivariate logistic regressions assessed odds of achieving outcomes.

Results.
421 patients met inclusion (60.7 y, 81.8% female, CCI: 1.6, 27.1 kg/m2), 139 LC and 127 HC patients. After PSM, 102 patients remained in each cost group with an average reimbursement of LC: $12,494 versus HC: $29,248. Matched cohorts had similar demographics and baseline HRQLs. Matched groups had similar baseline SVA (HC: 59.0 vs. LC: 56.7 mm), PI-LL (HC: 13.1 vs. LC: 13.4°), and PT (HC: 25.3 vs. LC: 22.4°). Rates of complications were not significantly different between the cost groups. Compared to LC group, by 2Y HC patients had higher odds of reaching SCB in ODI (OR: 2.356, [1.220, 4.551], P=0.011), in SRS-Total (OR: 2.988, [1.515, 5.895], P=0.002), and in NRS Back (OR: 2.739, [1.105, 6.788], P=0.030). Similar findings were appreciated for HC patients in the setting of Schwab deformity outcome criteria.

Conclusions.
Although added cost did not guarantee an ideal outcome, HC patients experienced superior patient reported outcomes compared to LC patients. While cost efficiency remains an important priority for health policy, isolating cost reduction may compromise outcomes and add to future costs of re-intervention, particularly with more severe baseline deformity.

Level of Evidence.
III
Interleukin-1 Blockers for the Treatment of Recurrent Pericarditis: Pathophysiology, Patient-Reported Outcomes, and Perspectives
Authors: Thomas Georgia K. , Bonaventura Aldo , Vecchié Alessandra , van Tassell Benjamin , Imazio Massimo , Klein Allan , Luis Sushil Allen , Abbate Antonio 
Published: May 2023

Abstract
Recurrent pericarditis (RP) is the most troublesome complication of acute pericarditis reflecting an unresolving inflammation of the pericardial sac around the heart and associated with significant morbidity. Recent studies have shown interleukin-1 (IL-1) signaling to be central to the pathophysiology of cases of RP with evidence of activation of systemic inflammation. We herein review the literature and clinical trials discussing the utility of IL-1 blockade for RP. The early experience of IL-1 blockade with anakinra (Kineret) and its favorable safety profile paved the way for the clinical development of rilonacept (Arcalyst) and subsequent approval by the US FDA for RP. In patients with RP who have become colchicine-resistant and glucocorticoid-dependent, IL-1 blockade with rilonacept or anakinra effectively treats recurrences and prevents future flares and significantly improves quality of life.
Quality Matters: Examining the Impact of Nurse Staffing Challenges on Clinical Outcomes
Authors: Polancich Shea , Miltner Rebecca , Montgomery Aoyjai , Dick Tracey , Poe Terri , Brown Daran , Patrician Patricia A. 
Published: May 2023

Goal:
The purpose of this study was to examine nurse staffing while describing the relationships that exist in staffing and quality associated with nursing care during the COVID-19 pandemic, a significantly challenging time for nurse staffing. We examined the relationship between permanent registered nurse (RN) and travel RN staffing during the pandemic and the nursing-sensitive outcomes of catheter-associated urinary tract infections (CAUTIs), central line-associated bloodstream infections (CLABSIs), falls, and hospital-acquired pressure injuries (HAPIs) and length of stay and compared the cost of CAUTIs, CLABSIs, falls, and HAPIs in fiscal years 2021 and 2022.

Methods:
We used a descriptive, observational design to retrospectively examine permanent nurse staffing volume and CAUTI, CLABSI, HAPI, and fall counts from October 1, 2019, to February 28, 2022, and travel nurse volume for the most current 12 months, April 1, 2021, to March 31, 2022. Descriptive statistics, Pearson correlation, and statistical process control analyses were completed.

Principal Findings:
Pearson correlation showed a statistically significant, moderately strong negative correlation (r = −0.568, p = .001) between the active registered nurse full-time equivalents (RN FTEs) and average length of stay (ALOS), and a moderately strong positive correlation (r = 0.688, p = .013) between the travel RN FTEs and ALOS. Pearson correlations were not statistically significant, with low to moderate negative correlations for CAUTIs (r = −0.052, p = .786), CLABSIs (r = −0.207, p = .273), and falls (r = −0.056, p = .769). Pearson correlation for active RN and HAPI showed a moderately strong, statistically significant positive correlation (r = 0.499, p = .003). We observed common cause variation in CAUTIs and CLABSIs, with HAPIs and falls showing special cause variation via statistical process control.

Practical Applications:
Despite the challenges associated with the lack of available nurse staffing accompanied by increasing responsibilities including unlicensed tasks, positive clinical outcomes can be maintained by staff adherence to evidence-based quality improvement.
The Patient Experience of Adolescents With Traumatic Injuries: Recommendations for Improvement
Authors: McCord Emma , Ballard Joseph H. , Mills Carol A. , Bandali Elhaam , Bell Teresa M. , Mazurenko Olena 
Published: May 2023

Goal:
Positive patient experience is associated with less healthcare utilization, better treatment adherence, increased likelihood of returning to the same hospital, and fewer complaints. However, hospitals have been able to collect few insights into the experiences of pediatric patients due to age limitations. As an exception to that reality, adolescents (aged 12–20 years) are able to share their experiences and recommend improvements, yet little is known about their hospital experiences with traumatic injuries. We examined the patient experience of adolescents with traumatic injuries and collected their recommendations for improving care.

Methods:
We conducted 28 semistructured interviews with English-speaking adolescents hospitalized at two trauma Level 1 hospitals (pediatric and adult) for physical injuries from July 2018 to June 2021. Interviews were transcribed and analyzed using modified thematic analysis.

Principal Findings:
The patients expressed three basic desires: (1) autonomy and active involvement in their care, (2) human connections with their clinicians, and (3) minimal discomfort. Study participants provided actionable recommendations for improving the patient experience for adolescents with traumatic injuries.

Practical Applications:
Hospital administrators and clinicians can improve the patient experience for adolescents in their care by sharing information, expectations, and goals with them. Hospital administrators can also empower the clinical staff to connect with adolescents with traumatic injuries on a personal level.
Transoral endoscopic thyroidectomy vestibular approach as a safe and feasible alternative to open thyroidectomy: a systematic review and meta-analysis
Authors: Oh Moon Young , Chai Young Jun , Yu Hyeong Won , Kim Su-Jin , Choi June Young , Lee Kyu Eun 
Published: May 2023

Background:
Transoral endoscopic thyroidectomy vestibular approach (TOETVA) is a scarless alternative to open thyroidectomy (OT). This systematic review and meta-analysis aimed to synthesize evidence comparing the intraoperative and postoperative outcomes of TOETVA and OT.

Methods:
A systematic literature search of PubMed, Web of Science, the Cochrane Library, and Google Scholar was performed to identify studies comparing the outcomes of TOETVA and OT published before February 2023. The outcomes of interest were operative time, intraoperative blood loss, hospital stay, postoperative pain, number of central lymph nodes retrieved, number of metastatic central lymph nodes, and incidences of transient and permanent recurrent laryngeal nerve injury, transient and permanent hypocalcemia, hematoma, and infection.

Results:
Thirteen studies published between 2016 and 2022, involving a total of 2889 patients (TOETVA, n=1085; OT, n=1804) were included in this systematic review and meta-analysis. Meta-analysis showed that the TOETVA group had a significantly longer overall operative time (weighted mean difference [WMD] 55.19; 95% CI, 39.15, 71.23; P<0.001), longer hospital stay (WMD, 0.27; 95% CI, 0.14, 0.39; P<0.001), and lower pain scores on postoperative day 1 (WMD, −1.41; 95% CI, −2.79, −0.03; P=0.04) than the OT group. Other intraoperative and postoperative outcomes were not significantly different between the groups.

Conclusion:
TOETVA has a similar safety profile to OT with less postoperative pain, making it an appropriate and more cosmetically appealing alternative to OT for select patients.
Postoperative infectious complications following laparoscopic versus open hepatectomy for hepatocellular carcinoma: a multicenter propensity score analysis of 3876 patients
Authors: Pu Jia-Le , Xu Xiao , Chen Lan-Lan , Li Chao , Jia Hang-Dong , Fan Zhong-Qi , Li Ju-Dong , Guan Ming-Cheng , Liang Ying-Jian , Zhou Ya-Hao , Wang Xian-Ming , Gu Wei-Min , Wang Hong , Li Jie , Chen Zhi-Yu , Chen Ting-Hao , Zhang Yao-Ming , Chen Zi-Xiang , Yao Lan-Qing , Diao Yong-Kang , Wang Ming-Da , Shen Feng , Pawlik Timothy M. , Lau Wan Yee , Chen Zhong , Yang Tian , Lv Guo-Yue 
Published: May 2023

Objectives:Hepatocellular carcinoma (HCC) is a common indication for hepatectomy that is often complicated by postoperative complication. The authors sought to investigate the relationship between the open with laparoscopic approach of hepatectomy and incidences of postoperative infectious complications.Patients and methods:Using a multicenter database, HCC patients who underwent laparoscopic hepatectomy (LH) or open hepatectomy (OH) were reviewed and analyzed. Propensity score matching (PSM), inverse probability of treatment weight (IPTW), and multivariate logistic regression analyses were utilized to assess the association of the operative approach with postoperative infectious complications, including incisional surgical site infection (SSI), organ/space SSI, and remote infection (RI).Results:Among 3876 patients, 845 (21.8%) and 3031 (78.2%) patients underwent LH and OH, respectively. The overall incidence of infection was 6.9versus14.6% among patients who underwent LHversusOH, respectively (P<0.001). Of note, the incidences of incisional SSI (1.8 vs. 6.3%,P<0.001), organ/space SSI (1.8 vs. 4.6%,P<0.001), and RI (3.8 vs. 9.8%,P<0.001) were all significantly lower among patients who underwent LHversusOH. After PSM (6.9, 1.8, 1.8, and 3.8% vs. 18.5, 8.4, 5.2, and 12.8%, respectively) and IPTW (9.5, 2.3, 2.1, and 5.5% vs. 14.3, 6.3, 4.5, and 9.8%, respectively), LH remained associated with statistically lower incidences of all types of infectious complications. After adjustment for other confounding factors on multivariate analyses, LH remained independently associated with lower incidences of overall infection, incisional SSI, organ/space SSI, and RI in the overall, PSM, and IPTW cohorts, respectively.Conclusion:Compared with open approach, laparoscopic approach was independently associated with lower incidences of postoperative infectious complications following hepatectomy for HCC.
Comparisons of different new-generation transcatheter aortic valve implantation devices for patients with severe aortic stenosis: a systematic review and network meta-analysis
Authors: Yang Yi-Xing , Liu Xin-Ming , Fu Yuan , Li Chuang , Wang Hong-Jiang , Xu Li , Xia Kun , Zhang Zhi-Yong , Zhong Jiu-Chang , Chen Mu-Lei , Su Pi-Xiong , Wang Le-Feng 
Published: May 2023

Background:Whether there are differences among the new-generation transcatheter aortic valve implantation (TAVI) devices for patients with aortic stenosis remains unclear. The aim of the study was to compare the efficiency and safety of different new-generation TAVI devices for patients with aortic stenosis.Materials and methods:A comprehensive search of PubMed, Embase and Web of Science from their inception to 1 February 2022. Randomized clinical trials and observational studies that compared two or more different TAVI devices were enroled. Pairwise meta-analysis and frequentist network meta-analysis were conducted to pool the outcome estimates of interest.Results:A total of 79 studies were finally included. According to the surface under the cumulative ranking, the top two ranked valves for lower rates of events were as follows: direct flow medical (DFM) (4.6%) and Lotus (48.8%) for lower rate of device success; Sapien 3 (16.8%) and DFM (19.7%) for lower mortality; DFM (8.6%) and Sapien 3 (25.5%) for lower rates of stroke; Evolut (27.6%) and DFM (35.8%) for lower rates of major and life-threatening bleeding; Portico (22.6%) and Sapien 3 (41.9%) for lower rates of acute kidney injury; Acurate (8.6%) and DFM (13.2%) for lower rates of permanent pacemaker implantation; Lotus (0.3%) and Sapien 3 (22.7%) for lower rates of paravalvular leak; Evolut (1.4%) and Portico (29.1%) for lower rates of mean aortic valve gradients.Conclusions:The findings of the present study suggested that the device success rates were comparable among these new-generation valves except for DFM. After excluding DFM, Sapien 3 might be the best effective for decreased mortality and stroke; Lotus might be the best effective for decreased paravalvular leak; Evolut might be the best effective for decreased major and life-threatening bleeding and mean aortic valve gradients; Acurate and Portico might be the best effective for decreased permanent pacemaker implantation and acute kidney injury, respectively.
Quantification of institutional and professional impact of a professional advancement program for advanced practice registered nurses and physician assistants
Authors: Kuriakose Clair , Chan Garrett K. , Haven Angela , Remer Lauren , Milliken Amy , Majabo Baker Anita , Sen Krishneel , Tippett Vicki 
Published: May 2023

ABSTRACT
Professional advancement programs, also known as career or clinical ladders, have proliferated for advanced practice registered nurses (APRNs) and physician assistants (PAs) and recognize clinical and/or professional contributions within clinical agencies. Although the literature is rich in describing the benefits of these programs on job satisfaction and staff retention, there is a dearth of literature on the effect of these programs on clinical practice, institutions, and the respective professions. This article quantifies the impact on the institution and profession of APRNs and PAs who have been promoted through an institution's career ladder.
Asynchronous Learning Module for Pediatric Emergency Medicine Physicians on How to Manage Vaginal Birth and Neonatal Resuscitation in the Emergency Department
Authors: Nickerson Jillian , McCollum Nichole , Abdulrahman Eiman , Marko Kathryn , Soghier Lamia , Rucker Alexandra , Madati Jamil , Zhao Xian 
Published: May 2023

ObjectivesNewborn deliveries and neonatal resuscitation events are rare but essential skills for pediatric emergency medicine (PEM) physicians. We sought to evaluate the effect of an online module on PEM physicians' knowledge and confidence in managing newborn deliveries and neonatal resuscitation.MethodsA team of experts in PEM, obstetrics, neonatology, and medical education developed a self-directed, 1-hour online module on managing newborn deliveries with neonatal resuscitation. The module was designed to address the learning needs of the targeted group. The module was piloted before dissemination to PEM faculty. A 10-question multiple choice test was given to assess knowledge of the material covered. A 10-point Likert scale questions survey was used to evaluate confidence. Measures were administered before initiation, after module completion, and 6 months after completion. Pairedttests were used to compare mean knowledge scores, and rank sum tests were used to compare median confidence levels.ResultsMost (n = 47, 89%) of the PEM faculty members completed the module. The majority (n = 43, 91%) thought the information was relevant to their practice. After completing the module, physicians' overall knowledge scores improved by 18% (mean [SD]: 74% [14.7] vs 92% [8.0],P< 0.01). Self-assessed confidence improved after the module in terms of managing uncomplicated vaginal deliveries (median 5 vs 7,P< 0.01), care of patients with complicated vaginal deliveries (2 vs 5,P< 0.01), and managing neonatal resuscitation (7 vs 8,P< 0.01). During the 6-month follow-up, there was sustained improvement in physicians' overall knowledge score (82% [16.9],P= 0.007) and self-assessed confidence in managing complicated vaginal deliveries (median 2 vs 4,P= 0.0012); however, other measures were not statistically significant.ConclusionsAn online module is an appropriate method for training PEM providers about rarely used but essential skills such as managing vaginal deliveries and neonatal resuscitation.
“Frailty Indices Outperform Historic Risk Proxies as Predictors of Post-Abdominoplasty Complications: An Analysis of a National Database”
Authors: Akhavan Arya A. , Liu Helen , Alerte Eric , Ibelli Taylor , Etigunta Suhas K. , Katz Abigail , Kuruvilla Annet S. , Taub Peter J. 
Published: May 2023

Background:
While age, BMI, and major comorbidities were historically used as predictors of surgical risk, recent literature supports patient frailty as a more accurate predictor. Database studies and chart reviews support the modified Charlson Comorbidity Index (mCCI) and the Modified 5-Item Frailty Index (mFI-5) as predictors of postsurgical complications in plastic surgery. The authors hypothesized that the mFI-5 and mCCI are more predictive of abdominoplasty complications than historic risk proxies.

Methods:
A retrospective review of the NSQIP database was performed, for abdominoplasty patients from 2013 to 2019. Demographics, comorbidities, and complications were gathered. The mFI-5 and mCCI scores were calculated per patient. Age, BMI, major comorbidities, ASA class, mFI-5 score, and mCCI score were compared as predictors of all-cause 30-day complications, 30-day surgical site complications, length of stay, and aggregate Clavien-Dindo complication severity score.

Results:
Of 421 patients, the strongest predictors for all-cause complications and complication severity were mCCI score ≥ 3 and mFI-5 score ≥2. mFI-5 score was the strongest predictor of unplanned reoperation. Length of stay was best predicted by age ≥ 65. The only predictor of surgical site complications was BMI ≥ 30.0. Smoking was predictive of complication severity, but not any other outcome.

Conclusions:
The mFI-5 and mCCI are stronger outcome predictors than historically-used factors, which showed little predictive value in this cohort. While the mCCI is a stronger predictor than the mFI-5, the mFI-5 is easily calculated during an initial consultation. Surgeons can apply these tools to aid in risk stratification for abdominoplasty.