bluuebunny/crossref_metadata_2025_split huggingface dataset:
Establishment of TaqMan probe dual real-time PCR Assay for the Identification of NADC34-like PRRSV based on GP5 gene
Authors: Li Qunhui , Chang Hao , Qiu Yingwu , Wu Yu , Cao Xuezhen , Lin Limiao , Li Wei , Shen Hanqin , Zhou Qingfeng , Gong Lang , Wang Lianxiang 
Published: March 2023

Abstract
Porcine reproductive and respiratory disorder syndrome virus (PRRSV) is an RNA virus that can clinically cause reproductive disorders in sows and respiratory diseases in growing pigs. Recently, NADC34-like PRRSV has been reported to become prevalent gradually and an outbreak in pig farms in China. Additionally, there is no precise detection method for clinical testing. Therefore, in this study, a TaqMan probe dual real-time quantitative fluorescence detection method based on M and GP5 genes was developed to distinguish NADC34-like PRRSV by specific amplification of the GP5 gene. The method is strongly specific, only NADC34-like PRRSV was accurately detected. Besides, the maximum limit of detection of pMD-M and pMD-GP5 sensitivity was 2.67 × 102 copies/µL and 1.35 × 101 copies/µL, respectively. The method was reproducible and the coefficient of variation (CV) values of both recombinant plasmids were <2%. In the progress detection of 251 clinical samples, it was found that 27 NADC34-like PRRSV may be accurately detected by the method. Overall, this study was the first to establish an accurate and sensitive probe dual real-time PCR for NADC34-like PRRSV based on GP5 gene, which provides effective support and methods for clinical testing in pig farms.
Rumination and Insomnia in Chinese Adolescents with Mood Disorders: The Mediating Role of Anxiety
Authors: Liu Dianying , Lei Gang , Qiu Lijuan , Deng Hongdong , Liu Shaohua , Dang Yonghui , Zhang Xiang-Yang 
Published: March 2023

Abstract
Objective
Insomnia is the most common complaint in adolescents with mood disorders (MD). However, the psychopathological mechanisms associated with insomnia remain unclear. Therefore, we aimed to explore anxiety’s mediating role in the effect of rumination on insomnia in MD adolescents.
Methods
A total of 569 MD patients were recruited. Participants completed the Patient Health Questionnaire-9 (PHQ-9), 7-item Generalized Anxiety Disorder (GAD-7) scale, and Insomnia Severity Index (ISI) to self-assess their mood and insomnia symptoms. In addition, the 21-item Chinese version of the Ruminative Response Scale (RRS) was used to assess rumination specifically.
Results
The prevalence of insomnia in MD patients was 60.63%, with a higher prevalence in females (63.32%) vs. male patients(54.39%). MD patients with insomnia symptoms also scored higher on depression and anxiety symptom scales, as well as rumination, when compared to MD patients without insomnia. Depression-related rumination and anxiety were correlated with insomnia in MD patients. The AUCROC showed that the combination of depression-related rumination and anxiety could effectively distinguish patients with and without insomnia in MD adolescents. Furthermore, in adolescents with MD, depression-related rumination positively predicted anxiety and insomnia, and anxiety positively predicted insomnia. Finally, anxiety partially mediated the association between depression-related rumination and insomnia.
Conclusion
Our results suggest that depression-related rumination and anxiety are key riskfactors for insomnia in adolescents with MD. Furthermore, anxiety can exacerbate the effects of depression-related rumination on insomnia, suggesting that clinical interventions to reduce depression-related rumination and anxiety may be a viable consideration for insomnia in adolescents with MD.
Exploring the Effectiveness of Binary-Valued and Real-Valued Representations for Cross-Modal Retrieval
Authors: Bhatt Nikita , Bhatt Nirav , Prajapati Purvi 
Published: March 2023

AbstractCross-modal retrieval(CMR) refers to the task of retrieving semantically related items across different modalities. For example, given an image query, the task is to retrieve relevant text descriptions or audio clips. One of the major challenges in CMR is the modality gap, which refers to the differences between the features and representations used to encode information in different modalities. To address the modality gap, researchers have developed various techniques such as joint embedding, where the features from different modalities are mapped to a common embedding space where they can be compared directly. Binary-valued and real-valued representations are two different ways to represent data. A binary-valued representation is a type of discrete representation where data is represented using either 0 or 1. Real-valued representation, on the other hand, represents each item as a vector of real numbers. Both types of representations have their advantages and disadvantages, and researchers continue to explore new techniques for generating representations that can improve the performance of CMR systems. First time, the work presented here generates both the representations and comparison is made by performing experiments on standard benchmark datasets using mean average precision (MAP). The result suggest that real-valued representation outperforms binary-valued representation in terms of MAP, especially when the data is complex and high-dimensional. On the other hand, binary codes are more memory-efficient than real-valued embedding, and they can be computed much faster. Moreover, binary codes can be easily stored and transmitted, making them more suitable for large-scale retrieval tasks.
Case report: Response with chemotherapy and Olaparib in a patient with advanced cervical adenocarcinoma
Authors: Zhuang Jing , Qin Baoning , Yu Yang , Kou Zixing , Sun Chunjie , Meng Fanfan , Zhang Yaxuan , Li Ling 
Published: March 2023

Abstract
Background:
Cervical adenocarcinomas are a heterogeneous group of tumors, accounting for approximately 20% of all cervical cancers. Many clinical data suggest that the activity of PARPi inhibitors may not be limited to BRCA mutated tumours and may involve the homologous recombination pathway.
At present, there are few data available on the activity of PARPi in endometrial and cervical cancers. And poly-(ADP)-ribose polymerase inhibitors (PARPi) are changing clinical practice in the BRCA mutant patients with cervical cancer.
Case Description:
Here, we report the case of a 66-year-old female patient admitted to the hospital due to abnormal vaginal bleeding. She was diagnosed with advanced cervical adenocarcinomas, and received chemotherapy followed by Paclitaxe and Cisplatin in the first-line. However, after one cycle of treatment, the patient's disease progressed. To identify more opportunities for treatment, the biopsy tissue was genetic tested and found BRCA1 exon 23 mutation and PD-L1 positive score, which motivated chemotherapy combined with immune checkpoint inhibitors, and the tumor size decreased. However, because of the side effects of from long-term chemotherapy, the patient chosen Olaparib treatment in the later stage. The patient benefited long-term disease control for about 24 months.
Conclusions:
This case might support the strategy that PARPi could be an alternative treatment for cervical adenocarcinoma patients with BRCA1 mutation in the future.
The ratio of intratumoral CD15+ neutrophils to CD8+ lymphocytes predicts recurrence in gastric cancer patients after resection
Authors: Watanabe Junichiro , Kimura Takashi , Saze Zenichiro , Nishimagi Atsushi , Shigeyuki Tsukida , Suzushino Seiko , Muto Makoto , Sato Naoya , Kofunato Yasuhide , Ishigame Teruhide , Okada Ryo , Kenjo Akira , Shimura Tatsuo , Kono Koji , Marubashi Shigeru 
Published: March 2023

Abstract
An elevated neutrophil-to-lymphocyte ratio (NLR) in the peripheral blood is an independent prognostic indicator of various cancers. However, whether a similar immune cell balance in tumor tissues influences the prognosis of cancer patients is not well understood. This study aimed to investigate the prognostic relevance of the intratumoral immune cell balance in gastric cancer patients. The study included 82 consecutive patients who underwent curative resection for gastric cancer. Intratumoral Cluster of Differentiation (CD) 15- and CD8-positive cells were evaluated using immunohistochemical staining, and clinicopathological factors and prognoses were analyzed. The effects of these scores on overall survival (OS) and relapse-free survival (RFS) were estimated using Cox proportional hazards regression. Patients with high intratumoral CD15/CD8 ratios had significantly lower OS and RFS compared to those with low CD15/CD8 ratios (P ). In addition, a high CD15/CD8 ratio was associated with lymph node metastasis (P = 0.019). Patients with a high NLR had a significantly lower RFS than those with a low NLR (P = 0.0050). Multivariate analysis revealed that the intratumoral CD15/CD8 ratio was an independent prognostic indicator of RFS (Hazard ratio = 4.7, 95% confidence interval = 1.4–16.2). Therefore, an increased intratumoral CD15/CD8 ratio is an independent predictor of survival following gastric cancer resection and is associated with lymph node metastasis and microscopic lymph vessel invasion. Establishing the postoperative follow-up schedule and adjuvant therapy may be clinically advantageous.
Mendelian Randomization Study Suggests Circulating Copper Level as a Risk Factor for Liver Cirrhosis
Authors: Tang Xiaolong , Miao Yandong , Ha Wuhua , Li Zheng , Yang Lixia , Mi Denghai 
Published: March 2023

Abstract
Cirrhosis is a serious liver disease with an unclear etiology and pathogenesis. Previous studies suggest a correlation between circulating copper and cirrhosis. However, whether circulating copper is a risk factor for cirrhosis is currently controversial because the liver is a major organ of copper metabolism and cirrhosis affects copper circulation. To address this, we used a mendelian randomization to explore the effect of circulating copper concentration on the risk of cirrhosis. We selected instrumental variables (IVs) of circulating copper from genome-wide association studies and analyzed two datasets from FinnGen, one for cirrhosis in general and the other for cirrhosis caused by non-alcoholic fatty liver disease (NAFLD). The inverse-variance weighted method was primarily used for mendelian randomization analysis. We created two SNP IVs that were associated with circulating copper, and their genetic associations with cirrhosis were extracted from the two datasets. The cirrhosis-related dataset included 811 cirrhosis patients and 273,592 controls, while the dataset for cirrhosis caused by NAFLD included 437 cirrhosis patients and 216,861 controls. Mendelian randomization analysis predicted a significant association between higher levels of circulating copper and increased risk of cirrhosis of liver (OR = 1.82, 95% CI: 1.30 to 2.54, P < 0.001). Additionally, higher levels of circulating copper were also associated with increased risk of cirrhosis of liver caused by NAFLD (OR = 1.68, 95% CI: 1.08 to 2.60, P = 0.021). The study suggests that higher levels of circulating copper may be a pathogenic risk factor for cirrhosis, providing important insights for the prevention and treatment of cirrhosis.
Pesticide removal by the bioaugmentation process in secondary treated wastewater
Authors: Ammeri Rim Werheni , Kloula Salma , Simeone Giuseppe Di Rauso , Mehri Ines , Hassan Wafa , Hassen Abdennaceur 
Published: March 2023

Abstract

The work was focused on the effect of the bioaugmentation process on STWW contaminated by pentachlorophenol (PCP: 100 mg L−1) by Pseudomonas putida AE015451. The monitoring of bioaugmentation treatments was assessed by chloride content determination via high-performance liquid chromatography (HPLC), optical density (OD) for microbial biomass determination, and pyoverdine and biofilm production. The process of bioaugmentation by a PGPR Pseudomonas strain showed a high-efficiency removal rate of PCP (100 mg L−1). The contaminant decreased up to 92% after 168 h. The production of pyoverdine and the formation of bacterial biofilm by the strain Ps. putida AE015451 showed an important role in tolerating the toxicity of PCP by using it as a carbon source. The obtained result proved that the pyoverdine production and biofilm formation help the Pseudomonas bacteria to tolerate to the stressed condition as pesticide. Moreover, the co-existence of the iron and PCP molecule ameliorate its biodegradation.
The Feasibility of Interactive Multimedia and Online Quiz Based Gamification on Learning Management System (LMS) Thematic Learning Courses
Authors: Aprilia Tika , Ardiansyah Amalia Rizki , Riyanti Henni 
Published: March 2023

The global Covid-19 pandemic has had a variety of impacts on the education sector including the deepening of the learning process. The college has transformed initially face-to-face learning activities into distance learning using online learning platform. However, the design of the platform used is still less varied from the type of media and learning applications, where lectures should be able to provoke motivation and the active role of students. This research aims to find out the feasibility results of interactive multimedia and online quiz based on integrated gamification learning system in supporting thematic learning courses. This study used a research and development approach, specifically the ADDIE development model design by Branch (2009). The research subjects are 2nd semester students who will take Thematic Learning courses in Elementary School of Universitas Negeri Yogyakarta and Universitas PGRI Palembang. Data collection techniques use questionnaires and interviews. Data analysis uses descriptive analysis. The results showed that the validation results of media experts obtained a score of 3.88 with good categories and material expert validation obtained a score of 3.89 with a good category. Then the results of student responses to interactive multimedia products and online quiz based gamification at the time of the limited trial which included one to one trial obtained a score of 3.50 with a good category, small group trail obtained a score of 3.69 with a good category, and the field trial received a score of 3.95 with an very good category. Thus interactive multimedia and online quiz based gamification integrated LMS in thematic learning courses are said to be feasible and can be used in increasing student motivation and interaction in the lecture process.
An Intelligent System for Heart Disease Diagnosis using Regularized Deep Neural Network
Authors: Rani Pooja , Kumar Rajneesh , Jain Anurag 
Published: March 2023

Heart disease is one of the deadly diseases. Timely detection of the disease can prevent mortality. In this paper, an intelligent system is proposed for the diagnosis of heart disease using clinical parameters at early stages. The system is developed using the Regularized Deep Neural Network model (Reg-DNN). Cleveland heart disease dataset has been used for training the model. Regularization has been achieved by using dropout and L2 regularization. Efficiency of Reg-DNN was evaluated by using hold-out validation method.70% data was used for training the model and 30% data was used for testing the model. Results indicate that Reg-DNN provided better performance than conventional DNN. Regularization has helped to overcome overfitting. Reg-DNN has achieved an accuracy of 94.79%. Results achieved are quite promising as compared to existing systems in the literature. Authors developed a system containing a graphical user interface. So, the system can be easily used by anyone to diagnose heart disease using the clinical parameters.
PEMBUATAN APLIKASI RENCANA DETAIL TATA RUANG (RDTR) PERKOTAAN BERBASIS ANDROID
Authors: Yuni Mega Ningsih  , Mabrur Adkha Yulianandha , Feny Arafah  
Published: March 2023

Rencana Detail Tata Ruang (RDTR) adalah rencana secara terperinci tentang tata ruang wilayah kabupaten yang dilengkapi dengan peraturan zonasi kabupaten. Dengan perkembangan teknologi saat ini, maka diperlukan informasi persebaran Rencana Detail Tata Ruang (RDTR) dengan aturan zonasi yang ada di Perkotaan Lumajang melalui Sistem Informasi Geografis (SIG). Sehingga tujuan dari penelitian ini adalah memvisualisasikan data menjadi digital dan kemudian dikembangkan untuk mengakomodasi kebutuhan informasi yang diakses secara realtime dengan metode mobile GIS. Pembuatan aplikasi ini dibuat menggunakan software ArcGIS 10.3 sebagai pengolahan data dan MIT App Inventor 2 sebagai pembuatan bahasa pemrograman visual block dan PHP untuk pembuatan aplikasi android. Berdasarkan pembuatan aplikasi ini memiliki 9 (sembilan) desa/ kelurahan dengan data pola ruang yang terdiri dari 14 zona dan data 10 struktur objek yang tersebar pada zona. Hasil pembuatan aplikasi ini menampilkan tiga menu yang dapat di akses yaitu menu peta, menu fitur RDTR dan menu bantuan. Kelebihan dari menu peta yaitu menampilkan visualisasi data dengan beberapa fitur yang tersedia baik legenda, layers, rules, dan lokasi untuk membantu masayarat untuk mengetahui lokasi yang ada dilapangan dengan lokasi zona yang ada dengan memasukkan koordinat longitude dan latitude titik yang diinginkan. Sedangkan untuk menu fitur RDTR menampilkan data tabular data non spasial dan dapat dilakukan query untuk menampilkan informasi sesuai yang diinginkan masyarakat. Aplikasi RDTR Perkotaan Lumajang mendapat total persentase kelayakan sebesar 84% dan termasuk dalam kagetori sangat layak. Dari hasil pembuatan aplikasi ini diharapkan dapat membantu masyarakat untuk memberikan keterbukaan informasi publik terkait data RDTR Perkotaan Lumajang, serta memberikan kemudahan dalam akses data RDTR.
PELATIHAN PENGGUNAAN MEDIA PEMBELAJARAN ICT BERBASIS VIRTUAL CLASS UNTUK MENDUKUNG PEMBELAJARAN DARING PADA MASA PANDEMI COVID-19 BAGI GURU-GURU SMP/ SMA DI KOTA BANDAR LAMPUNG
Authors: Yarmaidi Yarmaidi , Zulkarnain Zulkarnain , Miswar Dedy , Utami Dian , Salsabilla Annisa , Tarkono Tarkono 
Published: March 2023

Kemampuan dan keterampilan guru baik menggunakan maupun mengadakan media pengajaran ICT berbasis virtual class dalam proses belajar mengajar di dalam kelas bukan lagi merupakan suatu hal yang dapat diabaikan, akan tetapi ini merupakan suatu keharusan, mengingat dalam era pembangunan pendidikan di masa sekarang dan mendatang bertitik tolak pada kualitas out-putnya. Tujuan diadakan kegiatan ini untuk meberikan pelatihan kepada guru-guru tentang pemanfaataan media pengajaran ICT berbasis virtual class dengan aplikasi Edmodo untuk melaksanakan pembelajaran secara daring (online). Hasil yang diperoleh dari kegiatan ini bahwa tingkat pengetahuan para peserta yang bersifat aspek pengetahuan atau kognitif tentang teori-teori dan konsep-konsep dasar Media Pembelajaran Berbasis ICT dapat dikatakan meningkat secara signifikan, peningkatan kognitif dicapai oleh lebih dari separuh peserta yang hadir, dan kelompok materi yang bersifat kesungguhan atau sikap, antusiasme peserta cukup tinggi. Simpulan dalam kegiatan ini bahwa dimasa yang akan datang pelatihan pembuatan media pembelajaran berbasis ICT bagi guru-guru perlu diperbanyak dan ditingkatkan lagi, minimal selama 4 minggu atau 1 bulan, khususnya bagi guru-guru yang mengajar materi di sekolah dasar dan menengah.
LUKISAN MURAL PENCEGAHAN COVID-19 SEBAGAI BENTUK EDUKASI KEPADA MASYARAKAT DESA RANTAU MINYAK
Authors: Perdana Yusuf , Adi Pratama Rinaldo , Saidi Subian , Febriansyah Febi Eka , Pratama Muhammad Ridho , Kusniar Dea , Irfani Azzahra Zatil , Febriani Ayu , Mulyani Anisa , Alikum Zatti , Yoga Muhamad , Tori Ferdinandus Ivanelian Jodie 
Published: March 2023

Dalam dunia seni visual, tentunya sudah tak asing lagi dengan yang namanya seni mural. Mural adalah salah satu karya seni yang cukup populer di Indonesia, terutama pada kalangan remaja. Seni mural juga seolah memberikan kebebasan bagi pembuatnya untuk mengeksplorasi kreatifitas yang dimilikinya lalu dicurahkan pada media yang permanen seperti dinding. Seni mural juga dapat dijadikan sebagai penunjuk tempat, history suatu tempat, atau bahkan media edukasi bagi masyarakat. Dengan masih adanya wabah Covid-19 terutama varian terbaru yaitu Omicron yang merajalela pada saat ini, mural menjadi andalan bagi mahasiswa sebagai media edukasi untuk pencegahan penularan wabah Covid-19 bagi masyarakat di salah satu desa Kabupaten Lampung Selatan yaitu Desa Rantau Minyak Kecamatan Candipuro. Oleh karena itu, kajian melalui penulisan ini dapat menjelaskan dengan lebih terperinci mengenai seni mural sebagai media edukasi bagi masyarakat di desa Rantau Minyak, Kecamatan Candipuro, Kabupaten Lampung Selatan.
PEMANFAATAN LIMBAH AMPAS TAHU MENJADI PRODUK INOVATIF "NUGGET AMPAS TAHU" UNTUK MENAMBAH PENDAPATAN EKONOMI MASYARAKAT PEDUKUHAN SILUWOK LOR
Authors: Kuncoro Sapto , Abdurrahim Mukhlis , Gisavana Finka , Alifiantoro Nurul Sharina , Saputri Dian Ristiani , Damayanti Vania Widyadhari , Febrianti Putri , Tunang Indika Poloriani , Anindyaguna Anggit , Rizani Joana Putri , Haq Faisal Rohmadhiyaul 
Published: March 2023

Pengabdian ini dilaksanakan di Pedukuhan Siluwok Lor, Kelurahan Tawangsari, Kecamatan Pengasih, Kabupaten Kulon Progo. Pengabdian ini bertujuan untuk mengembangkan ampas tahu menjadi produk nugget. Pengarajin industri tahu di Siluwok Lor ini belum memiliki pengetahuan tentang cara-cara penanganan ampas tahu yang baik. Limbah ampas tahu seringkali menimbulkan bau yang tidak sedap dan membuat lingkungan menjadi tampak kotor dan kumuh. Metode yang digunakan dalam penelitian ini adalah metode pendekatan dimana industri tahu dilibatkan langsung dalam proses pembuatan nugget ampas tahu. Sebelum dilaksanakan proses pembuatan, terlebih dahulu dilaksanakan pengarahan untuk memberikan pengetahuan terkait pemanfaatan ampas tahu dan prosedur pembuatan nugget ampas tahu. Hasil yang diperoleh dari penelitian ini adalah untuk mendapatkan tekstur yang baik dalam pembuatan Nugget ampas tahu adalah. ampas tahu 500 gram, telur 4 butir, tepung tapioka, tepung terigu, tepung panir, bawang merah 5 siung, bawang putih 4 siung, lada sdm, garam sdm, ketumbar 1 sdm, gula pasir 1 sdm, dan minyak goreng.
PENGARUH DOPING SBCL3 TERHADAP STRUKTUR DAN SIFAT OPTIK FILM TIPIS ZNO DENGAN METODE SOL-GEL SPIN COATING
Authors: Sirait Naomi 
Published: March 2023

Penelitian ini dilakukan untuk menghasil bahan baku pembuatan film tipis ZnO yang didoping Antimon (III) Klorida (SbCl3) sebagai bahan terbaik untuk didoping dengan ZnO. Film tipis ZnO doping SbCl3 telah berhasil disintesis dengan metode sol-gel spin coating dan proses pencampuran dengan teknik refluks. Bahan yang digunakan zinc acetat dehydrate {Zn(CH3COO)2 2H2O}, Antimoni (III) Klorida sebagai pendoping dilarutkan oleh pelarut isopropanol dengan variasi konsentrasi doping yaitu 4%; 5% dan 6% lalu distabilkan dengan diethanolamine (DEA). Pembuatan film tipis ZnO:SbCl3 ini dilakukan diatas substrat kaca dengan metode spin coating. Hasil karakterisasi film tipis ZnO:SbCl3­ dengan XRD menunjukkan ukuran kristal masing-masing adalah 26 nm, 26 nm, 24 nm. Dan hasil karakterisasi SEM menunjukkan permukaan morfologi film tipis ZnO:SbCl3 adanya retakan pada saat pelapisan dan bulatan hitam pada konsentrasi 5%. Hasil karakterisasi UV-VIS memperlihatkan nilai transmitansi dengan semua doping pada cahaya yang diteruskan pada lapisan ZnO doping SbCl3 lebih banyak daripada diserap pada rentang 350-800 nm. Dan nilai absorbansi menurun tajam pada rentang 350-800 nm. Nilai celah pita energi meningkat pada konsentrasi 5% dan menurun pada konsentrasi 6% dengan nilai Eg yaitu 3,03 eV.