bluuebunny/crossref_metadata_2025_split huggingface dataset:
Superconducting radio frequency linear collider HELEN
Authors: Belomestnykh S. , Bhat P.C. , Grassellino A. , Checchin M. , Denisov D. , Geng R.L. , Jindariani S. , Liepe M. , Martinello M. , Merkel P. , Nagaitsev S. , Padamsee H. , Posen S. , Rimmer R.A. , Romanenko A. , Shiltsev V. , Valishev A. , Yakovlev V. 
Published: September 2023

Abstract
This article discusses a proposed Higgs-Energy LEptoN
(HELEN) e
+
e
- linear collider based on advanced traveling wave
superconducting radio frequency technology. The proposed collider
offers cost and AC power savings, smaller footprint (relative to the
ILC), and could be built at Fermilab. After the initial physics run
at 250 GeV, the collider could be upgraded either to higher
luminosity or to higher, up to 500 GeV, energies.
AULA Outperforms RULA in Evaluating MSD Risks of Oyster Mushroom Farmers: Implications for Safer Farming Practices
Authors: Pawitra T A , Fathimahhayati L D , Fariza M A 
Published: September 2023

Abstract
This study aims to compare the suitability of two ergonomic evaluation tools, AULA and RULA, in assessing musculoskeletal disorders (MSD) potential risks in oyster mushroom farmers. The evaluation was based on 26 activities involved in the oyster mushroom cultivation process, observed in four farms in Lempake Samarinda, and assessed by 12 experienced experts in ergonomics, safety and health, and agriculture. The hit rate and quadratic weighted κ were used to compare the two methods. The results showed that AULA has a higher hit rate and Kappa value than RULA, indicating a moderate level of agreement and greater suitability for evaluating WMSDs of oyster mushroom farmers in Samarinda. This finding has implications for improving the safety and health of farmers in the agriculture sector.
Enhancing Red Ginger Plant Growth through Eco-Friendly Pest Management: Synergistic Effects of Metarhizium anisopliae and Imidacloprid
Authors: Solichah C , Poerwanto M E , Suryawati A 
Published: September 2023

Abstract
This study explores the potential of a more environmentally friendly pest management approach for enhancing the vegetative growth of red ginger plants (Zingiber officinale Rosc.) by mitigating the damage caused by Lepidiota stigma (white grub) larvae. The study was conducted at the Experimental Garden of the Faculty of Agriculture, UPN “Veteran” Yogyakarta from April to August 2022, utilizing a factorial Completely Randomized Design. The application of the entomopathogenic fungus Metarhizium anisopliae at varying doses (0 g, 10 g, 20 g, and 30 g per 3 kg of planting media) and the systemic insecticide Imidacloprid (either absent or at a dose of 0.02 g per 3 kg of planting media) were the primary factors studied. Each treatment combination, executed on ten plants each infested with one larvae, was repeated thrice. The findings demonstrate that a combined application of M. anisopliae and Imidacloprid could significantly reduce the intensity of white grub damage to red ginger plants and improve their vegetative growth. Notably, a dose of M. anisopliae of 20 g per 3 kg of planting media maximized the plant height, leaf number, and tiller count. The findings highlight the potential of integrating biological and chemical controls for sustainable pest management and crop yield improvement, with broad implications for other similar agricultural contexts.
Enhancing Nutritional Value and Safety in Horse Meat Cutlets with Pumpkin Additives
Authors: Abilmazhinov Y , Rebezov M , Fedoseeva N , Nikolaeva N , Sepiashvili E 
Published: September 2023

Abstract
This study introduces an innovative approach to meat cutlet production, integrating horse meat with a 20% pumpkin additive. To evaluate the nutritional impact of the pumpkin addition, two variants of cutlets were analyzed - a control variant devoid of pumpkin and an experimental variant supplemented with pumpkin. It was observed that the pumpkin additive enriched the cutlets with a significantly higher level of carbohydrates, minerals like calcium, and vitamins A and C (p<0.001). Simultaneously, critical control points (CCPs) in the production process were identified to mitigate potential hazards (biological, chemical, physical) at various stages, including raw meat receiving, storage, and preparation, grinding, mixing, packing, and freezing of the final product. The results underscore the potential of integrating vegetable additives like pumpkin into meat products not only for nutritional enhancement but also for effective hazard control and traceability throughout the production process, offering important implications for meat product innovation and safety protocols.
Improved Organoleptic Properties in Cooked Sausages through a Mutton-Poultry Blend: An Analytical Study
Authors: Kalibekkyzy Z , Neverova O , Rebezov M , Fedoseeva N , Vasilievich N 
Published: September 2023

Abstract
This study investigates the implications of utilizing a combination of mutton and poultry meat for cooked sausage production, with an emphasis on understanding changes in chemical composition and textural properties. Utilizing a systematic approach, four experimental variants were developed, progressively incorporating up to 30% poultry meat as a replacement for mutton. A comprehensive chemical analysis revealed an increase in the water-binding capacity, a notable decrease in fat content, and a slight increase in ash content (P<0.05 for both changes). In contrast, textural assessment showed no significant variations across the samples. The blending of mutton and poultry meat demonstrated an enhancement of organoleptic properties, inclusive of flavor, aroma, juiciness, and tenderness of the final product, without compromising the textural characteristics. The results of this study underline the potential benefits of integrating mutton and poultry in cooked sausage production, suggesting a novel pathway to create a nutritious, tasty, and high-quality product. The findings possess implications for the meat industry, providing scientific backing for the inclusion of diverse meats for an optimized product formulation.
Economic Feasibility of Modified Cassava Flour Milling in Gunungkidul Region, Indonesia: A Value-Added Agribusiness Venture
Authors: Herlianto D , Tahalea S P , Wibowo A W A , Rahatmawati I 
Published: September 2023

Abstract
This study embarked on the evaluation of the feasibility of constructing a Modified Cassava Flour (MOCAF) mill in the Gunungkidul Region of Indonesia, a significant cassava producer yet underutilizing its potential due to traditional, low-yield production techniques. The goal was to enhance MOCAF production and leverage its benefits, such as a low glycemic index, to meet contemporary dietary trends. Employing cost-benefit analysis, supported by key financial metrics including Net Present Value (NPV) and Payback Period (PP), we sought to establish the financial viability of this proposed agribusiness venture. Our analysis covered various factory feasibility aspects including market, marketing, technical, technological, human resources, management, environmental, legality, and risk factors. Results indicated the feasibility of the MOCAF mill construction in KWT Ngudi Rejeki, Ngestirejo, Tanjungsari, Gunungkidul DIY, both in operational and financial terms. The mill’s projected payback period was significantly shorter than the maximum threshold (6 years versus 10 years), and the NPV was positive at IDR 49,587,106 over ten years. This study implies that developing MOCAF mills could be a strategic move towards agro-industrial development and food security, advancing the value addition of cassava and providing an attractive investment opportunity for stakeholders in the agribusiness sector.
Assessing the Potential for an Agro-GeoHydroPark in Gedangrejo: An Integrated Geological and Agricultural Study
Authors: Kusumayudha S B , Setyaningrum T , Rahatmawati I , Probowati D , Budiman L , Nurwantari N A 
Published: September 2023

Abstract
This study aimed to evaluate the potential of Gedangrejo, located in the Karangmojo district, Gunung Kidul regency, Yogyakarta Special Region, Indonesia, for agro-geohydropark development. Employing a comprehensive geological analysis, which included assessing geomorphological, lithological, and geological structures alongside hydrogeological conditions, the study also focused on the evaluation of water sources and agricultural potential. Gedangrejo, which falls within the Baturagung subzone of the Southern Mountains zone, presents a unique amalgamation of undulating plains, hills, karst hills, and alluvium deposits with formations belonging to Semilir, Oyo, and Wonosari. Geological structures constituted by joints and faults, including three horizontal faults with a Northeast-Southwest orientation, were noted. The hydrogeology uncovered two aquifer systems, with the Semilir fissured inter-grain aquifer controlling the Baturagung Embung. In terms of agricultural viability, the area exhibits fertile land capable of supporting ornamental and fruit plants, presenting potential for fruit-picking tourism. Therefore, findings suggest that the Gedangrejo region holds substantial potential for development as an agro-geohydropark, implying a sustainable economic upliftment of the local community through the prudent utilization of natural resources and local wisdom.
Hidden Treasures of Kediri’s Medicinal Plants: A Collaborative Effort to Map and Validate Authentic Information Using Innovative QR Code Security and Cryptography
Authors: Sucipto  , Firliana R , Wardani A S , Muzzaki M N , Santoso A M , Forijati RR , Primandiri P R 
Published: September 2023

Abstract
This research aims to map the distribution of rare and medicinal plants in Kediri Regency, Indonesia, and develop an interactive database system to provide accurate information on these plants. The study was conducted in collaboration between Balitbangda Kediri and Nusantara University PGRI Kediri. The data collection utilized innovation in creating a web-based information system with QR code security and cryptography validation to ensure the authenticity of the plant data. The results of this research provide a comprehensive database of rare and medicinal plants in Kediri Regency that can support drug information, particularly during the Covid-19 pandemic. The interactive system facilitates the dissemination of accurate information and raises public awareness about the potential of medicinal and rare plants in the region. This research has significant implications for the conservation of rare and medicinal plants and their potential contribution to public health.
Efficient evaluation of natural stochastic policies in off-line reinforcement learning
Authors: Kallus Nathan , Uehara Masatoshi 
Published: September 2023

Summary
We study the efficient off-policy evaluation of natural stochastic policies, which are defined in terms of deviations from the unknown behaviour policy. This is a departure from the literature on off-policy evaluation that largely considers the evaluation of explicitly specified policies. Crucially, off-line reinforcement learning with natural stochastic policies can help alleviate issues of weak overlap, lead to policies that build upon current practice and improve policies’ implementability in practice. Compared with the classic case of a prespecified evaluation policy, when evaluating natural stochastic policies, the efficiency bound, which measures the best-achievable estimation error, is inflated since the evaluation policy itself is unknown. In this paper we derive the efficiency bounds of two major types of natural stochastic policies: tilting policies and modified treatment policies. We then propose efficient nonparametric estimators that attain the efficiency bounds under lax conditions and enjoy a partial double robustness property.
A-002 The Prevalence of the High Sensitivity Cardiac Troponin I Outlier and False Positivity Rates on Beckman Coulter Access DxI
Authors: Li X , Bryksin J 
Published: September 2023

Abstract

Background
The false positive elevation in high sensitivity cardiac troponin I (hsTnI) can be triggered by many factors, for example, fibrin clots, heterophile antibodies, or other sources of troponin interferences. These interferences lead to inaccurate hsTnI results. These inaccurate results described in the literature as the “outliers”. Published studies use statistical tools to identify the outliers based on the discrepancies between the two repeat values. Depending on the instrument platform and the study design, the outlier rate for the conventional troponin I assay was reported from 0.44% to 1.01% (1, 2). In our laboratory, the false positivity rate for the conventional troponin assay on Beckman Coulter DxI platform was determined as 1% (unpublished data). This study aim was to determine both the statistically and clinically significant outliers for hsTnI assay on the Beckman Coulter Access DxI instrument in the academic medical center clinical laboratory setting.

Methods
A unique test repeat protocol was created. Two parallel hsTnI measurements are conducted in lithium heparin tubes (BD Vacutainer) on Beckman Coulter DXI 800 systems. When the difference between the two results is greater than 30%, a third repeat is automatically ordered and run after additional centrifugation at a higher relative centrifugal force. The outlier determination is based on assessing the difference between the first two values using previously described statistical analysis tools (1). The clinically significant outlier was determined by counting positive results in the first two runs which are not unconfirmed by the third run, i.e., false positive results. Gender specific cutoffs, 14 ng/L for female and 19 ng/L for male, were applied to identify the positive results. Overall, 2935 patients’ results were evaluated during the study.

Results
Based on the analysis of the first two repeat runs, out of 2935 samples 16 samples, or 0.54%, were identified as the statistical outliers. 2 out of these 16 outliers, or 12.5%, were negative results. Taking into consideration the third repeat data, 13 out of 2935 samples, or 0.44%, were identified as false positive results. Only 9 out of 13 false positive results, or 69.2%, were identified using the statistical approach above.

Conclusion
The false positivity rate of hsTnI testing has dropped significantly from 1% to 0.44% in a setting of our laboratory. Our refined repeat protocol approach significantly increases the clinician’s confidences in the hsTnI results by capturing potential false positive results. This approach addresses the outliers at an additional expense like reagents, instrument wear and tear, and labor. Therefore, a more robust methodology is still desired by the industry.
A-054 Comparison of a Reformulated Vitamin D Immunoassay, Its Predecessor, and Two Contemporary Formulations
Authors: Geno K A , Alvarez Y F , Cervinski M A , Hubbard J A , Nerenz R D , Singh R J 
Published: September 2023

Abstract

Background
We took advantage of our verification of a new biotin-resistant 25-hydroxyvitamin D (vitamin D) immunoassay formulation to compare analytical performance of the outgoing formulation and assays from Roche, Diasorin and Beckman. Liquid chromatography with tandem mass spectrometry (LCMS) was an independent arbiter of vitamin D concentration and provided quantification of vitamin D2 and vitamin D3.

Methods
Twenty-five specimens from patients on high-dose vitamin D2 supplementation (50 000 IU/week) and fifty additional specimens spanning our measurable range were identified by the Dartmouth Hitchcock Analytics Institute. Aliquots were tested by the Roche cobas Vitamin D Total II and Vitamin D Total III assays, deidentified, split, and shipped to other study sites for measurement by LCMS, the Diasorin Liaison 25 OH Vitamin D Total assay, and the Beckman Coulter Access 25(OH) Vitamin D Total assay. Estimated vitamin D2 was calculated by subtracting the LCMS-measured vitamin D3 result from the immunoassay total vitamin D result. Bias calculations assume LCMS measurements as the true concentration. Statistical analyses were performed in R. This study was approved by the Dartmouth Hitchcock Medical Center Institutional Review Board.

Results
No significant differences were observed between platforms in absolute or relative bias in total vitamin D or estimated vitamin D2. Clinical classification was unanimous in 62/75 specimens. For thirteen specimens in which at least one disagreement was observed, immunoassay results for discrepant classification were no more than 10.2 ng/mL from the classification cutoff (median 2 ng/mL). Two specimens with 68 and 70 ng/mL vitamin D were classified as toxic (&gt;100 ng/mL) by one immunoassay each.

Conclusion
Current vitamin D immunoassays continue to improve over their predecessors and offer good agreement across platforms. Vitamin D2 recovery no longer represents a clinically-concerning obstacle. These immunoassays display positive bias for total vitamin D relative to LCMS but generally result in correct clinical classification.
A-155 Unlocking the Complete Blood Count: Derivation of a Single-Panel Laboratory Test That Includes Monocyte Distribution Width to Create a Universal Sepsis Screening Tool
Authors: Hinson J S , Sarani N , Smith A , Debraine A , Badaki-Makun O S , Malinovska A , Levin S 
Published: September 2023

Abstract

Background
Sepsis is the leading cause of in-hospital death. Outcome improvement requires rapid detection and early antimicrobial therapy. The complete blood count (CBC) is ordered for more than two-thirds of all patients in the emergency department (ED). Individual CBC components have limited utility for sepsis screening due to low specificity and sensitivity. We sought to create a reliable sepsis screening tool by combining components of the CBC differential.

Methods
In this single-center retrospective cohort study, all adult patients who had a CBC with differential performed within 6 h of ED arrival during a 20-month period were eligible for inclusion. Repeat ED visits by the same patient were excluded. Our primary outcome was manifestation of sepsis within 72 h of ED arrival, detected from timestamped electronic health records using a previously validated electronic phenotype based on Sepsis-3 consensus criteria. Multivariable logistic regression was performed to identify individual components of the CBC differential that were independently associated with our outcome and to identify optimal cutoffs for discretization. Odds ratios were used to assign weights to each component and to derive a 10-point system for scoring likelihood of sepsis.

Results
34 864 patients were included; 1306 (3.7%) met sepsis outcome criteria within 72 h of ED arrival and 181 (0.5%) had septic shock. White blood cell (WBC) count, monocyte distribution width (MDW), and the neutrophil to lymphocyte count ratio were found to predict sepsis. As shown in the figure, there was a linear relationship between our CBC infection severity index and likelihood of developing sepsis and septic shock. Patients with a high risk score (8 or above) had a greater than one in four chance of developing sepsis within 72 h.

Conclusion
A simple ten-point index derived from the most commonly used laboratory panel in medicine reliably estimated likelihood of developing sepsis.
A-246 Desialylation Profiles of Monocytes and Macrophages Upon LPS Stimulation
Authors: Aljohani M A , Sun X A , Zhao Y A 
Published: September 2023

Abstract

Background
Cell surface expresses a dense layer of glycans terminated with sialic acids (Sias), known as sialylation, which often represent different cellular statuses. Meanwhile, the removal of Sias from the glycans catalyzed by sialidase (desialylation) is also involved in many biological processes. Previous studies have established that lipopolysaccharide (LPS) can induce the expression of endogenous sialidases in monocytes and macrophages, leading to desialylation of the LPS receptor TLR4. This desialylation of TLR4 has been shown to impact the LPS/TLR4 signaling pathway. In this study, we conduct a profiling of desialylation in THP-1 monocytes and THP-1 macrophages in response to LPS stimulation. This will provide insights on the intricate connection between sialylation, desialylation, and LPS-induced signaling.

Methods
The activity of sialidases was assessed by utilizing 4-methylumbelliferyl-N-acetylneuraminic acid (4-MU-NANA) and ganglioside GM3 as substrates. The total sialic acid in the THP1 was quantified using liquid chromatography tandem mass spectrometry (LC-MS/MS). Flow cytometry was used to analyze the exposure of galactose on THP-1 monocytes and macrophages after LPS stimulation (100 ng/mL LPS for 24 h), using PNA-FITC staining. The expression level of Neu1 and Neu3 sialidases in cell lysates of THP-1 monocytes and macrophages were assessed by western blot technique after LPS stimulation. ELISA was used to measure the levels of sialidases in the cell supernatant of THP-1 monocytes and macrophages upon LPS stimulation.

Result
We found a significant reduction in total sialic acid levels in both THP-1 monocytes and macrophages upon LPS stimulation. This was further confirmed by the significant increase in total sialidase activities which measured by both 4-MU-Neu5Ac and GM3 substrates in THP-1 monocytes and macrophages upon LPS stimulation. Surprisingly, we also found that sialidase activities significantly increased in the cell supernatant of both THP-1 monocytes and macrophages, indicting sialidases secretion upon LPS stimulation. Western blot analysis further confirmed the high expression and secretion of both Neu1 and Neu3 sialidases from THP-1 monocytes and macrophages after LPS stimulation.

Conclusion
The present study conducted the first systematic profiling of desialylation and sialidase expression in monocytes and macrophages upon LPS stimulation. This results demonstrate that endogenous sialidase expression and secretion play a role in the LPS/TLR4 signaling pathway and subsequent cellular processes and functions in the context of inflammation and immune response.