bluuebunny/crossref_metadata_2025_split huggingface dataset:
Three-dimensional-printed porous prosthesis for the joint-sparing reconstruction of the proximal humeral tumorous defect
Authors: Zhang Yuqi , Lu Minxun , Hu Xin , Li Zhuangzhuang , Wang Jie , Gong Taojun , Zhou Yong , Luo Li , Min Li , Tu Chongqi 
Published: January 2023

Background: Tumorous bone defect reconstructions of the proximal humerus with joint sparing is a challenge. Numerous reconstruction methods have been proposed but the proximal residual humerus is commonly sacrificed because of its extremely short length. To preserve the proximal humerus and improve clinical outcomes, we designed a three-dimensional (3D) printed uncemented prosthesis with a porous structure to treat tumorous bone defects of the proximal humerus.Methods: Our analysis included seven patients treated between March 2018 and July 2019. A 3D model was established, and related data were obtained, including the diameter of the humeral head, the resection length, and the residual length. A prosthesis was designed and fabricated based on these data. Functional and oncologic outcomes were recorded, and complications and osseointegration were evaluated.Results: The mean age of the patients was 20.3 years, and the median follow-up period was 26 months. The lengths of the residual proximal humerus were 17.9 mm on average. All the patients had preserved humeral heads and most of the rotator cuff was intact. The average postoperative range of motion (ROM) of the affected shoulder was 83.8°; flexion was 82.5°, extension was 43.8°, and adduction was 16.3°. The average Musculoskeletal Tumor Society score (MSTS) was 94.3%. Good osseointegration was observed on the interface between the bone and prosthesis.Conclusion: A 3D printed porous prosthesis with cone-like structures successfully achieved joint-sparing reconstruction of proximal humeral tumorous defects with satisfying functional outcomes. The preservation of the rotator cuff and humeral head plays an essential role in the function of the shoulder joint.
Petrology and geochemical characteristics of amphibolite facies rocks in Xunyangba area, South Qinling Orogen, Central China
Authors: Kong Juanjuan , Wang Lei , Yao Yongxiang , Lin Jinyan , Xiao Yuanyuan , Yang Yiting 
Published: January 2023

As a tectonic block of the Qinling Orogenic Belt, Central China, the South Qinling Orogen is generally believed to have had a tight affinity with the Yangtze Block during the Precambrian. Knowledge on the South Qinling Orogen is important to constrain the tectonic evolution of the northern margin of the Yangtze Block basement. Previous studies mainly focused on the east segment of the South Qinling Orogen. This paper presented petrological, geochronological, and geochemical studies on the amphibolite-facies rocks newly discovered in the middle section of the South Qinling Orogen. Geochemical studies indicate that the protolith of these amphibolites is calc-alkaline island arc basalt, which was formed during the reconstruction of the Paleo-Middle Archean crust on the northern margin of the Yangtze Block 2,362 ± 100 Ma ago (equivalent to metamorphic rocks of the Yudongzi group). Later, these arc basalts experienced amphibolite-facies metamorphism at 1,500–1,800 Ma, which is consistent with the metamorphism identified in the Yangtze Block, confirming the association of the South Qinling Orogen with the Yangtze Block basement. Subsequently, these amphibolite-facies rocks further experienced three stages of metamorphism at c. 800 Ma, 440 Ma, and 200 Ma in the context of the northward subduction of the Yangtze Block.
Two different types of provenances and the amalgamation of subduction complexes in the Eastern Tianshan of the Southern Altaids
Authors: Mao Qigui , Xiao Wenjiao , Sang Miao , Ao Songjian , Song Dongfang , Tan Zhou , Wang Hao , Li Rui 
Published: January 2023

The nature and final closure of the northern Tianshan Ocean have been debated in regard to the eastern Tianshan orogen, southern Altaids. The Kanguer subduction complex of the eastern Tianshan is the key to addressing these issues. In this study, we report new mapping, geochemical and geochronological results on the Kanguer subduction complex in the Haluo area. Our new results show that upper Permian (257 Ma) basaltic blocks emplaced in a sandstone matrix in the northern HL area are fragments of normal-mid-ocean-ridge-basalt (N-MORB)-type oceanic crust. The geochronological results indicate that the sandstone matrices display two different types of provenances. The first type in the northern part of the cross-section (till Sample YY12) has maximum depositional ages ranging from 316 Ma to 238 Ma. Their depositional settings varied from intraoceanic island arcs to continental Andean arcs after ca. 244 Ma, their detrital zircon age patterns vary from a single peak to multiple peaks, and their zircon εHf(t) values vary from uniquely high positive to some negative values. The second type is in the southern part of the cross-section with Samples YY12 to 21Kg05, which have the geochemical signatures of continental island arc sandstone, multiple-peak detrital zircon-age patterns, and positive to negative zircon εHf(t) values. The youngest sandstone sample has a maximum depositional age of 241 Ma. The above provenance results indicate that all mélanges and coherent units north of Sample YY12 belong to an accretionary complex of the Dananhu intraoceanic arc, and those south of Sample YY12 belong to an accretionary complex of the Yamansu-central Tianshan arc. According to the youngest components in both accretionary complexes, which suggest the latest subduction events, we conclude that the final amalgamation timing was after 238 Ma and that the Paleo-Asian Ocean closed during the Middle to Late Triassic.
Fission-track thermochronological evidence for the Yanshanian tectonic evolution of the northern Junggar Basin, northwest China
Authors: Li Zhenhua , Chen Zhanjun , Fan Yuhai , Yu Lan , Zhang Suya , Li Xiangyang 
Published: January 2023

The Junggar Basin is a multicycle intracontinental sedimentary basin developed on the pre-Mesozoic deformed basement. For a long time, the Junggar Basin and its adjacent Altai orogeny have been a focus of debate for geologists studying the opening and closing history of the Paleozoic Asian Ocean and Cenozoic intracontinental deformation. However, there has been no detailed research on the intracontinental tectonic activities of northern Xinjiang since the Mesozoic, particularly the Yanshanian tectonic activities in the northern Junggar Basin. Fission-track (FT) dating was conducted on 15 apatite samples and eight zircon samples obtained from the northern Junggar Basin to better understand the Yanshanian tectonic evolution. The results showed that apatite FT (AFT) ages ranged from 131 to 42 Ma and zircon FT ages ranged from 205 to 132 Ma. Based on the AFT track thermal history modeling and the regional geological data, we proposed that the northern Junggar Basin underwent three tectonic thermal events during 165–161, 93–81, and 72–66 Ma. The thermal events of 165–161 Ma may indicate magmatic activity during the Yanshanian, while the 93–86 and 72–66 Ma events reflect Late Cretaceous uplift and cooling. This study has confirmed the tectonic evolution of the Yanshanian in the northern Junggar Basin from the perspective of thermochronology. It has also revealed that the Yanshanian orogeny, a regional tectonic event, may have also occurred in northwest China.
Integration of solar based charging station in power distribution network and charging scheduling of EVs
Authors: Shafiq Aqib , Iqbal Sheeraz , Rehman Anis Ur , Elbarbary Z. M. S. , Kotb Hossam , Selim Ali , Kamel Salah 
Published: January 2023

The rapid development of electric vehicles (EVs) such as Easy Bike, Auto-Rickshaw, and Electric Bike is a major contributor to global energy concerns. Although electric vehicles are bringing a new dimension to the transportation sector, with advantages such as being the cheapest method of transportation and emitting fewer greenhouse gases (GHGs), the massive amounts of energy required to charge the electric vehicles is a challenging issue. Pakistan is also moving toward the use of electric vehicles however the absence of charging facilities in Pakistan slows down the charging process and increases the prices for electric vehicle users. Finding the requisite charging without threatening the current power infrastructure is one of the most challenging tasks of the present era. Renewable energy-based charging is required to fulfill the charging demand of electric vehicles. To find the best configuration to meet the necessary daily charging demand, this proposed work undertakes a techno-economic assessment for a novel renewables-based grid-tied charging station. The technical, economic and environmental impacts of Solar based grid-tied charging stations are taken into account. Moreover, the results are justified by considering the losses and building the system model. The suggested strategy decreases energy costs from $.200/kWh to $.016/kWh while reducing grid load by 254,030 kWh/yr. Furthermore, the system completes 7.7 charging sessions every day, using 13% of the electricity generated. The remaining 87% of the electricity is sold back to the grid, which generates significant revenue.
Exploring the prognostic value of HK3 and its association with immune infiltration in glioblastoma multiforme
Authors: Yang Yuling , Fu Xing , Liu Runsha , Yan Lijuan , Yang Yiping 
Published: January 2023

Background: Hexokinase 3 (HK3) is one of the key enzymes involved in glucose phosphorylation (the first step in most glucose metabolic pathways). Many studies have demonstrated the vital role of dysregulation of HK3 in several tumors. However, there is a need for in-depth characterization of the role of HK3 in glioblastoma multiforme (GBM).Methods: All data were sourced from The Cancer Genome Atlas (TCGA) and Chinese Glioma Genome Atlas (CGGA). Kaplan-Meier analysis and univariate regression were applied for survival analysis. Gene set enrichment analysis (GSEA) was used for enrichment analysis. Tumor Immune Single Cell Hub (TISCH) database was applied for single-cell analysis. Tumor Immune Dysfunction and Exclusion (TIDE) analysis was applied to evaluate the immune response.Results: HK3 expression was upregulated in GBM and correlated with poor prognosis. The high HK3 expression group was primarily enriched in adaptive immune response, chemokine signaling pathway, and cytokine-cytokine receptor interaction. The high HK3 expression group showed significantly greater enrichment of the majority of immune cells and immune-related pathways. HK3 showed significant correlation with most immune cells, especially macrophages (p &lt; .001, R = .81). TISCH analysis showed that HK3 was predominantly expressed in macrophages in most cancers. HK3 showed significant correlation with most immune-related genes, such as PD-1 (p &lt; .001, R = .41), PDL-1 (p &lt; .001, R = .27), and CTLA-4 (p &lt; .001, R = .29). TIDE analysis revealed that the low HK3 expression group has a lower TIDE score and may benefit from immunotherapy. Drug sensitivity analysis showed that patients with high HK3 expression frequently showed drug resistance.Conclusion: HK3 was associated with poor prognosis and may serve as a biomarker of macrophages in GBM. HK3 was also associated with immune response and drug resistance. Our findings may provide novel insights for GBM immunotherapy.
Mitochondrial genome characteristics and phylogenetic analysis of the medicinal and edible plant Mesona chinensis Benth
Authors: Tang Danfeng , Huang Suhua , Quan Changqian , Huang Yuan , Miao Jianhua , Wei Fan 
Published: January 2023

Mesona chinensis Benth (MCB) (or Platostoma palustre or Platostoma chinense) is an important edible and medicinal plant in China. However, the mitochondrial genome (mitogenome, or mtDNA) of MCB has not been characterized or reported yet. In this study, we first sequenced and characterized the complete mitogenome of MCB. The MCB mitogenome was 494,599 bp in length and encoded 59 genes containing 37 protein-coding genes (PCGs), 19 tRNAs, and 3 rRNAs. Gene transfer analysis revealed that a total of 12 transfer segments with more than 93% identity (total length of 25,427 bp) were detected in the MCB mitogenome. Simple sequence repeats (SSR) analysis showed that 212 simple sequence repeats (SSR) were identified. Repeat sequence analysis revealed 305 repeat sequences (158 forward and 147 palindromic repeats) ranging from 30 bp to 48,383 bp and the 30–39 bp repeats were the majority type. Relative synonymous codon usage (RSCU) analysis uncovered that in total, 9,947 codons were encoding the protein-coding genes (PCGs). Serine (909, 9.1%) and leucine (879, 8.8%) were the two most abundant amino acids, while terminator (32, .3%) was the least abundant amino acid. Ka/Ks analysis indicated that almost all genes were subject to purification selection, except ccmB. Analysis of Lamiaceae mitogenomes constitution revealed that atpB and atpE were unique to the Rotheca serrata and Salvia miltiorrhiza mitogenomes. mttB gene loss was unique to the Boea hygrometrica mitogenome. The core fragments of the Lamiaceae mitogenomes harbored a higher GC content than the specific and variable fragments. In addition, phylogenetic analysis revealed that MCB was closely related to Salvia miltiorrhiza based on the mitogenomes. The current study provided valuable genomic resources for understanding and utilizing this important medicinal plant in the future.
Individual effects of GSTM1 and GSTT1 polymorphisms on cervical or ovarian cancer risk: An updated meta-analysis
Authors: Ye Jing , Mu Yi-Yang , Wang Jiong , He Xiao-Feng 
Published: January 2023

Background: Studies have shown that glutathione S-transferase M1 (GSTM1) and. glutathione S-transferase T1 (GSTT1) null genotype may increase the risk of cervical cancer (CC) or ovarian cancer (OC), however, the results of published original studies and meta-analyses are inconsistent.Objectives: To investigate the association between GSTM1 present/null and GSTT1 present/null polymorphisms, with the risk of cervical cancer or ovarian cancer.Methods: The odds ratios (ORs) and 95% confidence intervals (CIs) were used to assess the association between GSTM1 present/null and GSTT1 present/null polymorphisms and the risk of cervical cancer or ovarian cancer. To assess the confidence of statistically significant associations, we applied false positive reporting probability (FPRP) and bayesian false discovery probability (BFDP) tests.Results: Overall analysis showed that GSTM1 null was associated with an increased risk of cervical cancer, and subgroup analysis showed a significant increase in cervical cancer risk in Indian and Chinese populations; GSTT1 was not found null genotype are significantly associated with cervical cancer. Overall analysis showed that GSTM1 and GSTT1 null were not associated with the risk of ovarian cancer, subgroup analysis showed that GSTM1 null was associated with an increased risk of OC in East Asia, and GSTT1 null was associated with an increased risk of OC in South America. However, when we used false positive reporting probability and bayesian false discovery probability to verify the confidence of a significant association, all positive results showed “low confidence” (FPRP &gt; .2, BFDP &gt; .8).Conclusion: Overall, this study strongly suggests that all positive results should be interpreted with caution and are likely a result of missing plausibility rather than a true association.
A cellular senescence-related genes model allows for prognosis and treatment stratification of hepatocellular carcinoma: A bioinformatics analysis and experimental verification
Authors: Li Jiaming , Tan Rongzhi , Wu Jie , Guo Wenjie , Wang Yupeng , You Guoxing , Zhang Yuting , Yu Zhiyong , Geng Yan , Zan Jie , Su Jianfen 
Published: January 2023

Introduction: Hepatocellular carcinoma (HCC) is the most common type of primary liver cancer with low 5-year survival rate. Cellular senescence, characterized by permanent and irreversible cell proliferation arrest, plays an important role in tumorigenesis and development. This study aims to develop a cellular senescence-based stratified model, and a multivariable-based nomogram for guiding clinical therapy for HCC.Materials and methods: The mRNAs expression data of HCC patients and cellular senescence-related genes were obtained from TCGA and CellAge database, respectively. Through multiple analysis, a four cellular senescence-related genes-based prognostic stratified model was constructed and its predictive performance was validated through various methods. Then, a nomogram based on the model was constructed and HCC patients stratified by the model were analyzed for tumor mutation burden, tumor microenvironment, immune infiltration, drug sensitivity and immune checkpoint. Functional enrichment analysis was performed to explore potential biological pathways. Finally, we verified this model by siRNA transfection, scratch assay and Transwell Assay.Results: We established an cellular senescence-related genes-based stratified model, and a multivariable-based nomogram, which could accurately predict the prognosis of HCC patients in the ICGC database. The low and high risk score HCC patients stratified by the model showed different tumor mutation burden, tumor microenvironment, immune infiltration, drug sensitivity and immune checkpoint expressions. Functional enrichment analysis suggested several biological pathways related to the process and prognosis of HCC. Scratch assay and transwell assay indicated the promotion effects of the four cellular senescence-related genes (EZH2, G6PD, CBX8, and NDRG1) on the migraiton and invasion of HCC.Conclusion: We established a cellular senescence-based stratified model, and a multivariable-based nomogram, which could predict the survival of HCC patients and guide clinical treatment.
Identifying and validating molecular subtypes of biliary atresia using multiple high-throughput data integration analysis
Authors: Wang Dingding , Yang Shen , Zhao Yong , Zhang Yanan , Hua Kaiyun , Gu Yichao , Li Shuangshuang , Liao Junmin , Yang Ting , Zhao Jiawei , Huang Jinshi 
Published: January 2023

BackgroundBiliary atresia (BA) is the most common form of severe neonatal obstructive jaundice. The etiology and pathogenesis of BA are multifactorial, and different factors may interact to produce heterogeneous pathological features and clinical outcomes. Despite different pathological features, all patients received the same treatment strategy. This study performed integrative clustering analysis based on multiple high-throughput datasets to identify the molecular subtypes of BA and provide a new treatment strategy for personalized treatment of the different subtypes of BA.MethodsThe RNA sequence dataset GSE122340 in the Gene Expression Omnibus (GEO) database was downloaded; 31 BA and 20 control normal liver tissues were collected at our center for transcriptome sequencing, and clinical and follow-up data of BA patients were available. Molecular subtypes were identified using integrated unsupervised cluster analysis involving gene expression, biliary fibrosis, and immune enrichment scores based on the transcriptome dataset, and the results were validated using independent datasets.ResultsBased on the results of the integrated unsupervised clustering analysis, four molecular subtypes were identified: autoimmune, inflammatory, virus infection-related, and oxidative stress. The autoimmune subtype with a moderate prognosis was dominated by autoimmune responses and morphogenesis, such as the Fc-gamma receptor and Wnt signaling pathway. The biological process of the inflammatory subtype was mainly the inflammatory response, with the best prognosis, youngest age at surgery, and lowest liver stiffness. The virus infection-related subtype had the worst prognosis and was enriched for a variety of biological processes such as viral infection, immunity, anatomical morphogenesis, and epithelial mesenchymal transition. The oxidative stress subtype was characterized by the activation of oxidative stress and various metabolic pathways and had a poor prognosis. The above results were verified independently in the validation sets.ConclusionsThis study identified four molecular subtypes of BA with distinct prognosis and biological processes. According to the pathological characteristics of the different subtypes, individualized perioperative and preoperative treatment may be a new strategy to improve the prognosis of BA.
Differential expression profiles and functional prediction of circRNA in mice with traumatic heterotopic ossification
Authors: Wang Zheng , Yi Xinzeyu , Liu Yuhang , Liu Qiaoyun , Li Zonghuan , Yu Aixi 
Published: January 2023

BackgroundTraumatic heterotopic ossification (HO) is an intractable sequela incited by inflammatory insult. To date, the exact molecular mechanisms of traumatic HO formation remain unclear. Recent studies have indicated that circular RNAs (circRNAs) participate in various human skeletal diseases. Although the formation of HO recapitulates many programs during bone development and remodeling, few data are available concerning whether circRNAs could participate in this pathological osteogenesis.MethodsTo investigate the differentially expressed circRNAs (DE-circRNAs) in HO formation, microarray assay was performed to analyze the circRNA expression profile in four pairs of mice HO tissues and normal tissues. Then, qRT-PCR was applied to verify the microarray data. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses showed the biological functions of the differentially expressed circRNAs target genes. Cytoscape software was used to construct the circRNA–miRNA–mRNA network for circRNAs with different expression levels as well as the target genes.ResultsWe demonstrated that 491 circRNAs were significantly differentially expressed in mouse HO tissues by a fold-change ≥ 2 and p-value ≤ 0.05. Among them, the expressions of 168 circRNAs were increased, while 323 were decreased. The expression levels of 10 selected circRNAs were verified successfully by qRT-PCR. GO analysis exhibited that these DE-circRNAs participated in a series of cellular processes. KEGG pathway analysis revealed that multiple upregulated and downregulated pathways were closely related to the DE-circRNAs in HO mice. The circRNA-miRNA-mRNA networks demonstrated that DE-circRNAs may be involved in the pathological osteogenesis of HO through the circRNA-targeted miRNA-mRNA axis.ConclusionOur study first demonstrated the expression profiles and predicted the potential functions of DE-circRNAs in mice traumatic HO, which may shed new light on the elucidation of mechanisms as well as provide novel potential peripheral biological diagnostic markers and therapeutic targets for traumatic HO.