bluuebunny/crossref_metadata_2025_split huggingface dataset:
An interprofessional school‐based initiative to increase access to oral health care in underserved Florida counties
Authors: Morón Elías M. , Singer Richard 
Published: March 2023

AbstractOBJECTIVETo examine and describe the effect of an oral health education program on school‐based nurses' acquisition of oral health knowledge.METHODSThree‐hour synchronous videoconference sessions provided training for nurses to conduct oral health risk assessments, screen for oral diseases, deliver oral health education, apply fluoride varnish, and refer children identified in need of further assessment and treatment to a dentist. Oral health knowledge acquisition was assessed by comparing pre‐training and post‐training examination scores. Analyses included descriptive statistics and the Wilcoxon signed‐rank test.RESULTSSeventeen nurses from Suwannee, Lafayette, and Hamilton counties participated in the oral health education training program. Analyses of the school‐based nurses' test results showed a significant increase in correct answers on the post‐training test (93%) compared to the pre‐training test (56%). Six hundred forty‐one children from six elementary public schools received oral health education, oral screenings, and fluoride varnish applications. Fifty‐eight percent of the children had untreated caries, 43% had treated caries, 15% had sealant on permanent molars, and 3% required urgent care. Nurses successfully referred children identified in need of further assessment and treatment to a dentist.CONCLUSIONSThe synchronous videoconference oral health training program was effective in improving school‐based nurses' oral health knowledge. The knowledge acquired by school‐based nurses via oral health training programs can be leveraged to increase access to oral health care for vulnerable and unserved school‐aged populations.
Long‐term ear‐EEG monitoring of sleep – A case study during shift work
Authors: Jørgensen Sofie Drevsholt , Kidmose Preben , Mikkelsen Kaare , Blech Maxine , Hemmsen Martin Christian , Rank Mike Lind , Kjaer Troels Wesenberg 
Published: March 2023

SummaryThe interest in sleep as a potential clinical biomarker is growing, but the standard method of sleep assessment, polysomnography, is expensive, time consuming, and requires a lot of expert assistance for both set‐up and interpretation. To make sleep analysis more available both in research and in the clinic, there is a need for a reliable wearable device for sleep staging. In this case study, we test ear‐electroencephalography. A wearable, where electrodes are placed in the outer ear, as a platform for longitudinal at‐home recording of sleep. We explore the usability of the ear‐electroencephalography in a shift work case with alternating sleep conditions. We find the ear‐electroencephalography platform to be reliable both in terms of showing substantial agreement to polysomnography after long‐time use (with an overall agreement, using Cohen's kappa, of 0.72) and by being unobtrusive enough to wear during night shift conditions. We find that fractions of non‐rapid eye movement sleep and transition probability between sleep stages show great potential as sleep metrics when exploring quantitative differences in sleep architecture between shifting sleep conditions. This study shows that the ear‐electroencephalography platform holds great potential as a reliable wearable for quantifying sleep “in the wild”, pushing this technology further towards clinical adaptation.
Dynamic personalized prediction of the individual liver‐related risk after sustained viral response in HCV patients
Authors: Moreau Clémence , Roux Marine , Riou Jérémie , Canivet Clémence M. , Audureau Etienne , Lusivika‐Nzinga Clovis , Nahon Pierre , Carrat Fabrice , Boursier Jérôme ,   the ANRS CO12 CirVir group and the ANRS CO22 HEPATHER group
Published: March 2023

AbstractSustained viral response (SVR) significantly improves the prognosis in patients with hepatitis C virus (HCV) chronic infection but does not totally alleviate the risk of liver‐related complications (LRC). We aimed to evaluate whether the dynamics of multiple measurements of simple parameters after SVR enable the development of a personalized prediction of prognosis in HCV patients. HCV mono‐infected patients who experienced SVR in two prospective cohorts (ANRS CO12 CirVir cohort: derivation set; ANRS CO22 HEPATHER cohort: validation set) were included. The study outcome was LRC, a composite criterion including decompensation of cirrhosis and/or hepatocellular carcinoma. Joint latent class modelling accounting for both biomarker trajectory and event occurrence during follow‐up was developed in the derivation set to compute individual dynamic predictions, with further evaluation in the validation set. In the derivation set (n = 695; 50 LRC during the median 3.8 [1.6–7.5] years follow‐up), FIB4 was identified as a biomarker associated with LRC occurrence after SVR. Joint modelling used sex and the dynamics of FIB4 and diabetes status to develop a personalized prediction of LRC. In the validation set (n = 7064; 273 LRC during the median 3.6 [2.5–4.9] years follow‐up), individual dynamic predictions from the model accurately stratified the risk of LRC. Time‐dependent Brier Score showed good calibration that improved with the accumulation of visits, justifying our modelling approach considering both baseline and follow‐up measurements. Dynamic modelling using repeated measurements of simple parameters predicts the individual residual risk of LRC and improves personalized medicine after SVR in HCV patients.
Spermidine suppresses the activation of hepatic stellate cells to cure liver fibrosis through autophagy activator MAP1S
Authors: Shi Boyun , Wang Wei , Ye Mengting , Liang Min , Yu Ziyu , Zhang Yingying , Liu Zhaoyu , Liang Xue , Ao Jian , Xu Fengfeng , Xu Guibin , Jiang Xianhan , Zhou Xinke , Liu Leyuan 
Published: March 2023

AbstractBackground and AimsLiver diseases present a wide range of fibrosis, from fatty liver with no inflammation to steatohepatitis with varying degrees of fibrosis, to established cirrhosis leading to HCC. In a multivariate analysis, serum levels of spermidine were chosen as the top metabolite from 237 metabolites and its levels were drastically reduced along with progression to advanced steatohepatitis. Our previous studies that showed spermidine supplementation helps mice prevent liver fibrosis through MAP1S have prompted us to explore the possibility that spermidine can alleviate or cure already developed liver fibrosis.MethodsWe collected tissue samples from patients with liver fibrosis to measure the levels of MAP1S. We treated wild‐type and MAP1S knockout mice with CCl4‐induced liver fibrosis with spermidine and isolated HSCs in culture to test the effects of spermidine on HSC activation and liver fibrosis.ResultsPatients with increasing degrees of liver fibrosis had reduced levels of MAP1S. Supplementing spermidine in mice that had already developed liver fibrosis after 1 month of CCl4 induction for an additional 3 months resulted in significant reductions in levels of ECM proteins and a remarkable improvement in liver fibrosis through MAP1S. Spermidine also suppressed HSC activation by reducing ECM proteins at both the mRNA and protein levels, and increasing the number of lipid droplets in stellate cells.ConclusionsSpermidine supplementation is a potentially clinically meaningful approach to treating and curing liver fibrosis, preventing cirrhosis and HCC in patients.
Bulk RNA‐seq analyses of mandibular condylar cartilage in a post‐traumatic TMJ osteoarthritis rabbit model
Authors: Tosa Ikue , Ruscitto Angela , Wang Ziyi , Chen Kira Z. , Ono Mitsuaki , Embree Mildred C. 
Published: March 2023

AbstractObjectiveThe temporomandibular joint (TMJ) is anatomically comprised of the mandibular condylar cartilage (CC) lined with fibrocartilaginous superficial zone and is crucial for eating and dental occlusion. TMJ osteoarthritis (OA) leads to pain, joint dysfunction and permanent loss of cartilage tissue. However, there are no drugs clinically available that ameliorate OA and little is known about global profiles of genes that contribute to TMJ OA. Furthermore, animal models that recapitulate the complexity of signalling pathways contributing to OA pathogenesis are crucial for designing novel biologics that thwart OA progression. We have previously developed a New Zealand white rabbit TMJ injury model that demonstrates CC degeneration. Here, we performed genome‐wide profiling to identify new signalling pathways critical for cellular functions during OA pathology.Materials and MethodsTemporomandibular joint OA was surgically induced in New Zealand white rabbits. Three months following injury, we performed global gene expression profiling of the TMJ condyle. RNA samples from TMJ condyles were subjected to sequencing. After raw RNA‐seq data were mapped to relevant genomes, differential expression was analysed with DESeq2. Gene ontology enrichment analysis and Kyoto Encyclopedia of Genes and Genomes pathway analysis were conducted.Results/ConclusionsOur study revealed multiple pathways altered during TMJ OA induction including the Wnt, Notch and PI3K‐Akt signalling pathways. We demonstrate an animal model that recapitulates the complexity of the cues and signals underlying TMJ OA pathogenesis, which is essential for developing and testing novel pharmacologic agents to treat OA.
Drouetiella epilithica sp. nov. and Drouetiella lurida (Oculatellaceae, Synechococcales) isolated in the Republic of Korea based on the polyphasic approach
Authors: Kim Do‐Hyun , Lee Nam‐Ju , Wang Hye‐Ryeung , Lim An Suk , Lee Ok‐Min 
Published: March 2023

SUMMARYFive strains of Drouetiella (ACKU666, 667, 668, 669 and 670) were isolated from gravels in water, stone monument and coastal mudflat in Korea, and were studied using morphological and molecular traits. All five strains had thin and simple trichomes and exhibited false branching. From these strains, four strains (ACKU666, 667, 668 and 669) exhibited similar cell lengths with reddish–brown colored cells such as Drouetiella lurida. The 16S rRNA gene phylogeny showed the four strains formed a clade with Drouetiella lurida, and their DNA similarity was calculated to be 99.1–100%. The color of strain ACKU670 appeared to be in bright blue–green color like Drouetiella fasciculata, and their thylakoids showed a parietal arrangement, which is a characteristic feature of the family Oculatellaceae. Strain ACKU670 turned out to be a sister clade to the D. lurida according to the phylogenetic analysis of the 16S rRNA gene. The 16–23S rRNA internal transcribed spacer secondary folding structure (D1–D1′, Box‐B and V3 helices) confirmed the uniqueness of strain ACKU670, therefore indicating differences from the related species. Considering all the results, we described our strain ACKU670 as Drouetiella epilithica sp. nov. in accordance with the International Code of Nomenclature for Algae, Fungi and Plants.
Agricultural producer service subsidies and agricultural pollution: An approach based on endogenous agricultural pollution
Authors: Li Xiaochun , Fu Huanan 
Published: March 2023

AbstractAgricultural producer service is an indispensable link in promoting agricultural modernization in developing countries, while agricultural pollution has become the largest source of pollution in many developing countries. Given that agricultural pollution is different from industrial pollution due to its endogenous characteristics, we linked agricultural pollution with the human capital level of labor and propose an endogenous model that reflects the agricultural pollution path, which is different from the traditional path. We studied the effects of government price subsidy, interest subsidy, and wage subsidy to agricultural producer service on agricultural pollution and other economic indicators by constructing a three‐sector general equilibrium model including agricultural producer service. We found that the rise of price subsidy for the agricultural producer service and the wage subsidy of the agricultural producer service sector would increase the output of agricultural sector and reduce the output of agricultural producer service under certain conditions, but aggravate agricultural pollution; at the same time, the rise of interest subsidy of the agricultural producer service sector would decrease the output of the agricultural sector, enhance the output of agricultural producer service conditionally, and decrease agricultural pollution. Preconditions of the three subsidies to improve national welfare have been obtained.
Disentangling working memory from multiple‐object tracking: Evidence from dual‐task interferences
Authors: Li Hui , Hu Luming , Wei Liuqing , He Hong , Zhang Xuemin 
Published: March 2023

Multiple object tracking (MOT) is generally regarded as a pure attention‐consuming task that draws heavily on attention resources. In the present study, we adopted a cross‐channel visual‐audio dual‐task paradigm, i.e., the MOT task combined with the concurrent auditory N‐back working memory task, to test whether working memory indeed plays a necessary role in the process of multiple tracking, as well as to further identify the specific types of working memory components involved in this process. Experiments 1a and 1b examined the relationship between the MOT task and nonspatial object working memory (OWM) processing by manipulating the tracking load and working memory load, respectively. Results in both experiments indicated that the concurrent nonspatial OWM task did not have a significant effect on the tracking capacity of the MOT task. In contrast, Experiments 2a and 2b examined the relationship between the MOT task and spatial working memory (SWM) processing by a similar approach. Results in both experiments indicated that the concurrent SWM task significantly impaired the tracking capacity of the MOT task, showing a gradual decrease with increasing SWM load. Overall, our study provides empirical evidence that multiple object tracking does involve working memory, primarily related to spatial working memory rather than nonspatial object working memory, which sheds more light on the mechanisms of multiple object tracking.
Well‐being during the coronavirus pandemic: The effect of big five personality and COVID‐19 beliefs and behaviors
Authors: Horwood Sharon , Anglim Jeromy , Bereznicki Hannah , Wood Joshua K. 
Published: March 2023

AbstractThis study examined the relationship between personality traits, COVID‐specific beliefs and behaviors, and well‐being during the COVID‐19 pandemic. In July 2020, at the onset of a second major lockdown, Australian adults (n = 1453) completed measures of Big Five personality, COVID beliefs and behaviors (i.e., belief in a rapid recovery, perceived risk, compliance, change in exercise, and change in interpersonal conflict), subjective well‐being and COVID‐specific well‐being. Personality correlates of COVID‐specific well‐being differed from those with general life satisfaction. The benefits of conscientiousness were elevated whereas the benefits of extraversion and agreeableness were reduced. Neuroticism was related to greater perceived risk from the pandemic, elevated interpersonal conflict during the pandemic, and more pessimistic views about the rate at which society would recover from the pandemic. In contrast, conscientiousness was notably related to greater compliance with directions from public health authorities. While regression models showed that general well‐being was largely explained by personality, COVID factors provided incremental prediction, and this was greatest when predicting COVID‐specific well‐being and lowest for global evaluations of life satisfaction. The observed prediction by beliefs and behaviors on well‐being beyond personality, provides potential opportunities for targeted interventions to support the management of future novel stressors.
An assessment of various pools of organic phosphorus distributed in soil aggregates as affected by long‐term P fertilization regimes
Authors: Khan Asif , Guo Shengli , Wang Rui , Zhang Shulan , Yang Xueyun , He Binghui , Li Tianyang 
Published: March 2023

AbstractThe phosphorus (P) forms in long‐term fertilization determine the fate and transport of P in soil. However, the fate of various pools of organic P of added P in the long‐term measured with sequential chemical fractionation is not well‐understood. Four soil physical aggregates (>250, 125–250, 63–125 and <63 μm) from 0‐ to 20‐cm depth after 35 years of long‐term fertilization treatments including control (CK), nitrogen and phosphorus fertilizer (NP) and NP combined with farmyard manure (NPM) under continuous winter wheat were separated using settling tube apparatus. Results showed that the application of long‐term P fertilization had no apparent effects on promoting the mass proportion of soil aggregates except for >250 μm, where the NP and NPM treatments significantly increased the mass proportion by 60% and 70% over CK, respectively. Compared with CK, P fertilizer (NP and NPM) treatments significantly increased organic P (Po) contents in each size aggregate. In particular, mean labile Po increased by 35% and 246%, moderately labile Po by 125% and 161%, nonlabile Po by 105% and 170% and total Po (TPo) by 101% and 178%, respectively, under NP and NPM treatments, respectively. There was a significant correlation between soil organic carbon (SOC) and Po fractions. SOC was exponentially positively correlated with labile Po but linearly positively correlated with moderately labile Po, nonlabile Po and TPo fractions among soil aggregates. A reduced C:Po ratio (<100) in soil aggregates among treatment indicates a large amount of available P accumulated in soils, and soil P loss risk in the study site is still high. Our results show that the Po pool measured by sequential chemical fractionation may represent an important, yet often overlooked, source of P in agriculture ecosystems. According to the result, long‐term mineral P fertilizer combined with organic amendments better sustains soil structural stability in large aggregates, contributing more Po availability in the moderately labile P followed by labile P in soil aggregates.
Continuous assessment of Peter Senge's learning organization model for improving the quality of Christian higher education in Indonesia
Authors: Simanjuntak Junihot M. , Heriyanto  , Hasibuan Nelson 
Published: March 2023

AbstractThis paper briefly discusses Peter Senge's Continuous Assessment Learning Organization Model for Quality Improvement, and describes how it is implemented in improving the quality of education by Christian Religious Colleges in Indonesia. The main conclusion of this paper is that Christian Religious Colleges in Indonesia must develop an organizational culture where continuous assessment for quality improvement is part of everyday practice in schools. To achieve this, it is proposed that schools should become learning communities based on Peter Senge's Learning Organization model. While this paper is not based on research, the main implication is that every school should seek creative ways to implement Peter Senge's Continuous Assessment Learning Organization Model for Quality Improvement according to its culture and spirituality. A second implication is a Christian learning organization should be a prayer‐defined organization. Every activity, every decision should be informed by prayer and be done in prayer, seeking the will of God, his approval, and his glory. Prayer should play a key role in any assessment. This conceptual paper is original as it explores the fit of applying Peter Senge's Continuous Assessment Learning Organization Model with seeking the will of God, his approval, and his glory for quality improvement in Christian Religious Colleges in Indonesia.
Impact of sternotomy and pericardiotomy on cardiopulmonary haemodynamics in a large animal model
Authors: Kirk Mathilde Emilie , Merit Victor Tang , Moeslund Niels , Dragsbaek Simone Juel , Hansen Jacob Valentin , Andersen Asger , Lyhne Mads Dam 
Published: March 2023

AbstractAnimal models of cardiovascular disease are often evaluated by invasive instrumentation for phenotyping. As no consensus exists, both open‐ and closed‐chest approaches are used, which might compromise rigour and reproducibility in preclinical research. We aimed to quantify the cardiopulmonary changes induced by sternotomy and pericardiotomy in a large animal model. Seven pigs were anaesthetized, mechanically ventilated and evaluated by right heart catheterization and bi‐ventricular pressure–volume loop recordings at baseline and after sternotomy and pericardiotomy. Data were compared by ANOVA or the Friedmann test where appropriate, with post‐hoc analyses to control for multiple comparisons. Sternotomy and pericardiotomy caused reductions in mean systemic (−12 ± 11 mmHg, P = 0.027) and pulmonary pressures (−4 ± 3 mmHg, P = 0.006) and airway pressures. Cardiac output decreased non‐significantly (−1329 ± 1762 ml/min, P = 0.052). Left ventricular afterload decreased, with an increase in ejection fraction (+9 ± 7%, P = 0.027) and coupling. No changes were observed in right ventricular systolic function or arterial blood gases. In conclusion, open‐ versus closed‐chest approaches to invasive cardiovascular phenotyping cause a systematic difference in key haemodynamic variables. Researchers should adopt the most appropriate approach to ensure rigour and reproducibility in preclinical cardiovascular research.
Did nutrient-rich oceans fuel Earth’s oxygenation?
Authors: Rasmussen Birger , Muhling Janet R. , Tosca Nicholas J. , Fischer Woodward W. 
Published: March 2023

Abstract
Phosphorus (P) availability exerts a strong influence on primary productivity in global oceans. However, its abundance and role as a limiting nutrient prior to the start of the Great Oxygenation Event (GOE) 2.45–2.32 Ga is unclear. Low concentrations of seawater P have been proposed to explain the apparent delay between the early appearance of oxygen-producing Cyanobacteria and the onset of atmospheric oxygenation. We report evidence for seawater precipitation of Ca-phosphate nanoparticles in 2.46–2.40 Ga iron formations deposited on a marine shelf, including shallow-water facies, immediately prior to the onset of the GOE. Our modeling shows that the co-precipitation of Ca-phosphate and ferrous silicate (greenalite) required ferruginous seawater with dissolved P concentrations many orders of magnitude higher than in today’s photic zone. If correct, it follows that P availability is unlikely to have suppressed the expansion of Cyanobacteria prior to the GOE. A reservoir of P-rich surface water shortly before 2.40 Ga could ultimately have triggered a rapid rise in atmospheric oxygen by fueling a sharp increase in primary productivity and organic-carbon burial. We speculate that the enigmatic Lomagundi positive carbon-isotope excursion, recorded in 2.32–2.06 Ga shallow-water carbonates, may mark a key step in the transition toward a modern biosphere of high biological productivity controlled by nutrient availability.