bluuebunny/crossref_metadata_2025_split huggingface dataset:
Validation of an Adapted Version of the Veterans RAND 12-Item Health Survey for Older Adults Living in Long-Term Care Homes
Authors: Wilson Rozanne , Cuthbertson Lena , Sasaki Ayumi , Russell Lara , Kazis Lewis E , Sawatzky Richard 
Published: March 2023

Abstract

Background and Objectives
The Veterans RAND 12-Item Health Survey (VR-12) is a generic patient-reported outcome measure of physical and mental health status. An adapted version of the VR-12 was developed for use with older adults living in long-term residential care (LTRC) homes in Canada: VR-12 (LTRC-C). This study aimed to evaluate the psychometric validity of the VR-12 (LTRC-C).

Research Design and Methods
Data for this validation study were collected via in-person interviews for a province-wide survey of adults living in LTRC homes across British Columbia (N = 8,657). Three analyses were conducted to evaluate validity and reliability: (1) confirmatory factor analyses were conducted to validate the measurement structure; (2) correlations with measures of depression, social engagement, and daily activities were examined to evaluate convergent and discriminant validity; and (3) Cronbach’s alpha (r) statistics were obtained to evaluate internal consistency reliability.

Results
A measurement model with 2 correlated latent factors (representing physical health and mental health), 4 cross-loadings, and 4 correlated items resulted in an acceptable fit (root-mean-square error of approximation = 0.07; comparative fit index = 0.98). Physical and mental health were correlated in expected directions with measures of depression, social engagement, and daily activities, though the magnitudes of the correlations were quite small. Internal consistency reliability was acceptable for physical and mental health (r &gt; 0.70).

Discussion and Implications
This study supports the use of the VR-12 (LTRC-C) to measure perceived physical and mental health among older adults living in LTRC homes.
Barriers and facilitators of implementing proactive deprescribing within primary care: a systematic review
Authors: Okeowo Daniel A , Zaidi Syed Tabish R , Fylan Beth , Alldred David P 
Published: March 2023

Abstract

Objective
Proactive deprescribing – identifying and discontinuing medicines where harms outweigh benefits – can minimise problematic polypharmacy, but has yet to be implemented into routine practice. Normalisation process theory (NPT) can provide a theory-informed understanding of the evidence base on what impedes or facilitates the normalisation of routine and safe deprescribing in primary care. This study systematically reviews the literature to identify barriers and facilitators to implementing routine safe deprescribing in primary care and their effect on normalisation potential using NPT.
PubMed, MEDLINE, Embase, Web of Science, International Pharmaceutical Abstracts, CINAHL, PsycINFO and The Cochrane Library were searched (1996–2022). Studies of any design investigating the implementation of deprescribing in primary care were included. The Mixed Methods Appraisal Tool and the Quality Improvement Minimum Quality Criteria Set were used to appraise quality. Barriers and facilitators from included studies were extracted and mapped to the constructs of NPT.

Key findings
A total of 12,027 articles were identified, 56 articles included. In total, 178 barriers and 178 facilitators were extracted and condensed into 14 barriers and 16 facilitators. Common barriers were negative deprescribing perceptions and suboptimal deprescribing environments, while common facilitators were structured education and training on proactive deprescribing and utilising patient-centred approaches. Very few barriers and facilitators were associated with reflexive monitoring, highlighting a paucity of evidence on how deprescribing interventions are appraised.

Summary
Through NPT, multiple barriers and facilitators were identified that impede or facilitate the implementation and normalisation of deprescribing in primary care. However, more research is needed into the appraisal of deprescribing post-implementation.
Exploring Corruption as a Violation of Human Rights in the Practices of International Human Rights Institutions
Authors: Juwita Ratna 
Published: March 2023

AbstractThis article discusses the evolution of soft law concerning the link between corruption and human rights. Evolution is defined as the increase in quantity and/or quality in International Human Rights Institutions’ normative outputs concerning corruption and human rights in (non-legally binding) instruments. Previous scholarly research was used as the benchmark to assess the evolution. The results indicated quantitative and qualitative evolutions in the production of the normative outputs regarding the link between corruption and human rights. The number of normative outputs produced by the respective institutions doubled and a substantial content-based evolution was identified. However, no normative output explicitly addressing corruption as a violation of human rights was found. Considering the pivotal role of the normative outputs to guide the implementation of human rights promotion and protection, this article suggests that the International Human Rights Institutions should continue to address and further clarify the link between corruption and human rights.
Perceptions of Adolescents With Chronic Pain About Peer Support: Reflexive Thematic Analysis
Authors: Linkiewich Delane , Dib Katherine C , Forgeron Paula A , Dick Bruce D , McMurtry C Meghan 
Published: March 2023

Abstract

Objective
Social challenges are a common experience for adolescents with chronic pain. Group peer support for these adolescents could be a promising intervention; however, no studies have focused exclusively on the peer support needs of this population. The present study addressed this gap in the literature.

Methods
Adolescents with chronic pain between 12 and 17 years of age completed a virtual interview and demographics questionnaire. Interviews were analyzed using inductive reflexive thematic analysis.

Results
Fourteen adolescents (Mage: 15.21 years; 9 females; 3 males, 1 nonbinary, 1 gender questioning) with chronic pain participated. Three themes were generated: “Being Misunderstood,” “They Would Understand Me,” and “Moving Forward Together in Our Pain Journeys.” Adolescents with chronic pain feel misunderstood and under supported by their peers without pain leading to feeling “othered” by having to explain their pain, yet not feeling free to talk about their pain with friends. Adolescents with chronic pain expressed that peer support would provide the forms of social support they are missing amongst their friends without pain as well as companionship and a sense of belonging due to shared knowledge and experiences.

Conclusions
Adolescents with chronic pain desire peer support from others like themselves, highlighting the challenges in their everyday friendships as the impetus for this support, as well as their anticipated short- and long-term benefits, including learning from their peers and developing new friendships. Findings indicate that adolescents with chronic pain may benefit from group peer support. Findings will inform the development of a peer support intervention for this population.
CRISPR/Cas-mediated in planta gene targeting: current advances and challenges
Authors: Guzmán-Benito Irene , Achkar Natalia Patricia , Bologna Nicolas G , Ursache Robertas 
Published: March 2023

Abstract
Gene targeting can be used to make modifications at a specific region in a plant’s genome and create high-precision tools for plant biotechnology and breeding. However, its low efficiency is a major barrier to its use in plants. The discovery of CRISPR (clustered regularly interspaced short palindromic repeats)–Cas-based site-specific nucleases capable of inducing double-strand breaks in desired loci resulted in the development of novel approaches for plant gene targeting. Several studies have recently demonstrated improvements in gene targeting efficiency through cell-type-specific expression of Cas nucleases, the use of self-amplified gene-targeting-vector DNA, or manipulation of RNA silencing and DNA repair pathways. In this review, we summarize recent advances in CRISPR/Cas-mediated gene targeting in plants and discuss potential efficiency improvements. Increasing the efficiency of gene targeting technology will help pave the way for increased crop yields and food safety in environmentally friendly agriculture.
Neglected acidity pitfall: boric acid-anchoring hole-selective contact for perovskite solar cells
Authors: Guo Huanxin , Liu Cong , Hu Honglong , Zhang Shuo , Ji Xiaoyu , Cao Xiao-Ming , Ning Zhijun , Zhu Wei-Hong , Tian He , Wu Yongzhen 
Published: March 2023

ABSTRACT
The spontaneous formation of self-assembly monolayer (SAM) on various substrates represents an effective strategy for interfacial engineering of optoelectronic devices. Hole-selective SAM is becoming popular among high-performance inverted perovskite solar cells (PSCs), but the presence of strong acidic anchors (such as –PO3H2) in state-of-the-art SAM is detrimental to device stability. Herein, we report for the first time that acidity-weakened boric acid can function as an alternative anchor to construct efficient SAM-based hole-selective contact (HSC) for PSCs. Theoretical calculations reveal that boric acid spontaneously chemisorbs onto indium tin oxide (ITO) surface with oxygen vacancies facilitating the adsorption progress. Spectroscopy and electrical measurements indicate that boric acid anchor significantly mitigates ITO corrosion. The excess boric acid containing molecules improves perovskite deposition and results in a coherent and well-passivated bottom interface, which boosts the fill factor (FF) performance for a variety of perovskite compositions. The optimal boric acid-anchoring HSC (MTPA-BA) can achieve power conversion efficiency close to 23% with a high FF of 85.2%. More importantly, the devices show improved stability: 90% of their initial efficiency is retained after 2400 h of storage (ISOS-D-1) or 400 h of operation (ISOS-L-1), which are 5-fold higher than those of phosphonic acid SAM-based devices. Acidity-weakened boric acid SAMs, which are friendly to ITO, exhibits well the great potential to improve the stability of the interface as well as the device.
The START domain potentiates HD-ZIPIII transcriptional activity
Authors: Husbands Aman Y , Feller Antje , Aggarwal Vasudha , Dresden Courtney E , Holub Ashton S , Ha Taekjip , Timmermans Marja C P 
Published: March 2023

Abstract
The CLASS III HOMEODOMAIN-LEUCINE ZIPPER (HD-ZIPIII) transcription factors (TFs) were repeatedly deployed over 725 million years of evolution to regulate central developmental innovations. The START domain of this pivotal class of developmental regulators was recognized over 20 years ago, but its putative ligands and functional contributions remain unknown. Here, we demonstrate that the START domain promotes HD-ZIPIII TF homodimerization and increases transcriptional potency. Effects on transcriptional output can be ported onto heterologous TFs, consistent with principles of evolution via domain capture. We also show the START domain binds several species of phospholipids, and that mutations in conserved residues perturbing ligand binding and/or its downstream conformational readout abolish HD-ZIPIII DNA-binding competence. Our data present a model in which the START domain potentiates transcriptional activity and uses ligand-induced conformational change to render HD-ZIPIII dimers competent to bind DNA. These findings resolve a long-standing mystery in plant development and highlight the flexible and diverse regulatory potential coded within this widely distributed evolutionary module.
Brassinosteroids regulate cotton fiber elongation by modulating very-long-chain fatty acid biosynthesis
Authors: Yang Zuoren , Liu Zhao , Ge Xiaoyang , Lu Lili , Qin Wenqiang , Qanmber Ghulam , Liu Le , Wang Zhi , Li Fuguang 
Published: March 2023

Abstract
Brassinosteroid (BR), a growth-promoting phytohormone, regulates many plant growth processes including cell development. However, the mechanism by which BR regulates fiber growth is poorly understood. Cotton (Gossypium hirsutum) fibers are an ideal single-cell model in which to study cell elongation due to their length. Here we report that BR controls cotton fiber elongation by modulating very-long-chain fatty acid (VLCFA) biosynthesis. BR deficiency reduces the expression of 3-ketoacyl-CoA synthases (GhKCSs), the rate-limiting enzymes involved in VLCFA biosynthesis, leading to lower saturated VLCFA contents in pagoda1 (pag1) mutant fibers. In vitro ovule culture experiments show that BR acts upstream of VLCFAs. Silencing of BRI1-EMS-SUPPRESOR 1.4 (GhBES1.4), encoding a master transcription factor of the BR signaling pathway, significantly reduces fiber length, whereas GhBES1.4 overexpression produces longer fibers. GhBES1.4 regulates endogenous VLCFA contents and directly binds to BR RESPONSE ELEMENTS (BRREs) in the GhKCS10_At promoter region, which in turn regulates GhKCS10_At expression to increase endogenous VLCFA contents. GhKCS10_At overexpression promotes cotton fiber elongation, whereas GhKCS10_At silencing inhibits cotton fiber growth, supporting a positive regulatory role for GhKCS10_At in fiber elongation. Overall, these results uncover a mechanism of fiber elongation through crosstalk between BR and VLCFAs at the single-cell level.
Phytochromes mediate germination inhibition under red, far-red, and white light in Aethionema arabicum
Authors: Mérai Zsuzsanna , Xu Fei , Musilek Andreas , Ackerl Florian , Khalil Sarhan , Soto-Jiménez Luz Mayela , Lalatović Katarina , Klose Cornelia , Tarkowská Danuše , Turečková Veronika , Strnad Miroslav , Mittelsten Scheid Ortrun 
Published: March 2023

Abstract
The view on the role of light during seed germination stems mainly from studies with Arabidopsis (Arabidopsis thaliana), where light is required to initiate this process. In contrast, white light is a strong inhibitor of germination in other plants, exemplified by accessions of Aethionema arabicum, another member of Brassicaceae. Their seeds respond to light with gene expression changes of key regulators converse to that of Arabidopsis, resulting in opposite hormone regulation and prevention of germination. However, the photoreceptors involved in this process in A. arabicum remain unknown. Here, we screened a mutant collection of A. arabicum and identified koy-1, a mutant that lost light inhibition of germination due to a deletion in the promoter of HEME OXYGENASE 1, the gene for a key enzyme in the biosynthesis of the phytochrome chromophore. koy-1 seeds were unresponsive to red- and far-red light and hyposensitive under white light. Comparison of hormone and gene expression between wild type and koy-1 revealed that very low light fluence stimulates germination, while high irradiance of red and far-red light is inhibitory, indicating a dual role of phytochromes in light-regulated seed germination. The mutation also affects the ratio between the 2 fruit morphs of A. arabicum, suggesting that light reception via phytochromes can fine-tune several parameters of propagation in adaptation to conditions in the habitat.
Phenytoin and carbamazepine cross reactivity: report of a case and review of literature
Authors: Misra U K , Kalita J , Rathore C 
Published: March 2023

Abstract
Cross reactivity between phenytoin, carbamazepine, and oxcarbazepine is reported. An 8 year old boy with partial seizures developed maculopapular rashes with itching on day 15 of carbamazepine therapy. After stopping carbamazepine, phenytoin 100 mg daily was prescribed two days later. On the 12th day of phenytoin therapy he developed cervical and axillary lymphadenopathy with fever. Lymph nodes revealed reactive hyperplasia. Oxcarbazepine 75 mg twice daily also resulted in oral and mucosa ulceration. The seizures were controlled without any side effects with sodium valproate 200 mg three times a day and gabapentin 300 mg twice a day. Due to the cross reactivity of aromatic anticonvulsants (phenytoin, carbamazepine, and oxcarbazepine), valproate or newer anticonvulsants should be used if a patient has sensitivity to these drugs.
Premature ejaculation prevalence among young men who have sex with men: a cross-sectional study with internet users in the metropolitan region of São Paulo, Brazil
Authors: de Mello Ferreira dos Reis Margareth , Barros Eduardo Augusto Corrêa , Monteiro Leonardo , Pazeto Cristiano Linck , Baccaglini Willy Roberto Camargo , Glina Sidney 
Published: March 2023

AbstractBackgroundThere are only a few studies about the prevalence and correlates of premature ejaculation (PE) among men who have sex with men (MSM).Aim(1) To estimate PE prevalence according to 3 assessment methods: self-reported time from penetration to ejaculation (ejaculation latency time [ELT]); Premature Ejaculation Diagnostic Tool (PEDT); and a direct question about the self-perception of ejaculation as being normal, too early (premature), or retarded. (2) To assess the agreement of the 3 assessment methods and identify factors associated with PE according to each method and their combination.MethodsWe evaluated data from 226 MSM who participated in a cross-sectional study about sexual behavior among men living in the metropolitan region of São Paulo, Brazil. They responded anonymously to an online survey between May 2019 and March 2020. We calculated the agreement of the 3 assessment methods and their association with other characteristics using logistic regression models.OutcomesOutcomes included the prevalence of PE according to the assessment methods and the association measures (PE vs sociodemographic characteristics and sexual behavior).ResultsThe prevalence of PE among MSM was 21.2% (95% CI, 16.1%-27.1%) according to the PEDT, 17.3% (95% CI, 12.6%-22.8%) per self-report, and 6.2% (95% CI, 3.4%-10.2%) by estimated ELT ≤2 minutes. The agreement among the 3 assessments was fair (kappa, 0.31; 95% CI, 0.25-0.37; P &lt; .001). Association with PE varied by assessment method: obesity and shorter time for ejaculation with anal sex vs masturbation were associated with PE according to the PEDT and ELT but not self-evaluation. Perception about ideal time to ejaculate ≤5 minutes increased the chance of PE based on ELT. Higher chances of self-reported PE were associated with trying to hold back ejaculation, and lower chances were associated with higher frequencies of masturbation.Clinical ImplicationsCombining tools to investigate PE allows the identification of characteristics associated with this condition and may result in improvement in the care of MSM.Strengths and LimitationsThis anonymous online survey provided the privacy necessary for participants to respond freely about sensitive questions, with a low risk of social adequacy bias. However, as it was a secondary analysis of a larger study, it could not evaluate comorbidities (eg, erectile dysfunction, prostatitis, depression) and the use of condoms.ConclusionThe prevalence of PE among MSM is high and varies according to the instrument used for the assessment, and the agreement among the 3 assessments was only fair.
Readability of the Orthopaedic Trauma Association Patient Education Tool
Authors: Ghanem Diane , Covarrubias Oscar , Harris Andrew B. , Shafiq Babar 
Published: March 2023

Introduction:
Online educational materials have become a fundamental resource for orthopaedic surgery patients; however, they are frequently written at a high level of reading ability and are too complicated for some patients to understand. The aim of this study was to appraise the readability of the Orthopaedic Trauma Association (OTA) patient education materials.

Methods:
All 41 articles on the OTA patient education website (https://ota.org/for-patients
) were analyzed for readability. Readability scores were calculated by 2 independent reviewers using the (1) FleschKincaid Grade Level (FKGL) and the (2) Flesch Reading Ease algorithms. Mean readability scores were compared across anatomic categories. One-sample t test was performed to compare mean FKGL with the recommended sixth-grade readability level and the average American adult reading level.

Results:
The average (SD) FKGL of the 41 OTA articles was 8.15 (1.14). The average (SD) Flesch Reading Ease for the OTA patient education materials was 65.5 (6.60). Four (11%) of the articles were at or below a sixth-grade reading level. The average readability of the OTA articles was significantly higher than the recommended sixth-grade level (P < 0.001; 95% confidence interval, 7.79–8.51). The average readability of the OTA articles was not significantly different from the average eighth-grade reading skill level of US adults (P = 0.41; 95% confidence interval, 7.79–8.51).

Conclusion:
Our findings suggest that despite most OTA patient education materials having readability levels suitable for the average US adult, these reading materials are still above the recommended sixth-grade level and may be too difficult for patient comprehension.
Methotrexate Drug Monitoring From Central Access: Can Blood Sample Collection From Central Venous Access Replace Peripheral Venipuncture in Adults?
Authors: Aydilek Enver , Schanz Julie , Brökers Nils 
Published: March 2023

Background:
Intravenous high-dose methotrexate (MTX ≥ 1 g/m2) is frequently used in patients with cerebral lymphoma or other malignancies. In addition to its potent efficacy, it is known to have pronounced toxicity and life-threatening side effects. Regular-level monitoring at short and defined intervals is mandatory. This study aimed to evaluate the possibility of replacing peripheral blood sampling with blood samples from central venous catheters for therapeutic monitoring of MTX in adults.

Methods:
A total of 6 patients and 7 cycles of chemotherapy (6 females; 5 with cerebral non-Hodgkin lymphoma and 1 with osteosarcoma, median age 51 years; range 33–62 years) were included. An immunoassay was used for quantitative analysis of MTX levels. The measurement points were obtained in the time intervals of 24, 42, 48, and 72 hours, and afterward, every 24 hours until the level was below <0.1 µmol/L. After flushing with 10 mL of saline solution and discarding 10 mL of venous blood, blood was drawn from the central venous access through which MTX had previously been administered. Simultaneously, MTX levels were obtained from peripheral venipuncture.

Results:
Methotrexate levels from central venous access and MTX levels from peripheral venipuncture showed a significant correlation (r = 0.998; P < 0.01; n = 35). During withdrawal from the central access group, 17 values showed a lower MTX level, 10 showed a higher level, and 8 showed no difference. However, the MTX level difference was not significant (P = 0.997, linear mixed model). No increase in the dose of calcium folinate was necessary based on the collected MTX levels.

Conclusions:
In adults, MTX monitoring from central venous access is not inferior to monitoring from peripheral venipuncture. Repeated venipuncture to measure MTX levels can be replaced after establishing standardized instructions for proper sampling by a central venous catheter.