bluuebunny/crossref_metadata_2025_split huggingface dataset:
Ultra-fast vitrification: Minimizing toxicity of cryoprotective agents and osmotic stress in mouse oocyte cryopreservation
Authors: Cho Jung Ran , Yu Eun Hee , Lee Hyun Joo , Kim In Hye , Jeong Ji Hye , Lee Dan Bi , Cho Seong Keun , Joo Jong Kil 
Published: August 2023

Abstract
Globally, women have been adopting oocyte cryopreservation (OC) for fertility preservation for various reasons, such as inevitable gonadotoxic treatment for specific pathologic states or social preferences. While conventional vitrification (C-VIT) technology has advanced the success rate of OC, challenges remain regarding the possible toxicities of high concentration cryoprotective agents and osmotic stress. To overcome such difficulties, we evaluated the ultra-fast vitrification (UF-VIT) method, which reduces non-vitrification stage exposure time compared to C-VIT, by observing mouse oocyte intracellular organelles. Consequently, compared to fresh mouse oocytes, UF-VIT had significant differences only in endoplasmic reticulum (ER) intensity and mitochondria (MT) distribution. Meanwhile, C-VIT showed significant differences in the survival rate and all ER and MT parameters. Among the two methods, UF-VIT had fewer damages than C-VIT in all MT parameters. Meiotic spindle (spindle and chromosome) morphology showed no significant changes between the groups during the vitrification/warming (VW) process, suggesting that the VW process did not harm the meiotic spindle of the oocytes. In conclusion, UF-VIT seems more effective in OC by efficient cytoplasmic water molecule extraction, osmotic stress reduction, and minimization of cell contraction and expansion amplitude, thus compensating for the drawbacks of C-VIT.
Unraveling the Molecular Links between Benzopyrene Exposure, NASH, and HCC: An Integrated Bioinformatics and Experimental Study
Authors: Yang Zheming , Li Jiayin , Song Haixu , Mei Zhu , Jia Xiaodong , Yan Chenghui , Han Yaling 
Published: August 2023

Abstract
Benzopyrene (B[a]P) is a widely recognized carcinogen that, upon chronic exposure, can induce chronic inflammation and fibrosis in liver tissue, ultimately resulting in liver disease. Nonalcoholic steatohepatitis (NASH) is a chronic liver condition characterized by fat accumulation, inflammation, and fibrosis, often resulting in hepatocellular carcinoma (HCC). This study aimed to investigate the intricate connections between B[a]P exposure, NASH, and HCC. Through comprehensive bioinformatics analysis, we identified differentially expressed genes (DEGs) associated with B[a]P exposure, NASH, and liver cancer using publicly available gene expression profiles. Subsequent network analysis revealed hub genes and protein-protein interactions, highlighting cellular metabolic dysfunction and disruption of DNA damage repair in the B[a]P-NASH-HCC process. Particularly, HSPA1A and PPARGC1A emerged as significant genes in this pathway. To validate their involvement, we performed qPCR in NASH mouse liver tissues and immunohistochemistry labeling in mouse and human HCC liver sections. Our findings providing crucial insights into the potential regulatory mechanisms underlying benzopyrene-induced hepatotoxicity. These results shed light on the pathogenesis of B[a]P-associated NASH and HCC and suggest that HSPA1A and PPARGC1A hold promise as therapeutic targets. Enhancing our understanding of their regulatory roles may facilitate the development of targeted therapies, leading to improved patient outcomes.
Examination of iatrogenic FDG accumulation after COVID-19 vaccination
Authors: Takahashi Keiko , Manabe Osamu , Shizukuishi Kazuya , Shibata Hiroshi , Kawakami Hiroki , Ohtsuka Akira , Oyama-Manabe Noriko 
Published: August 2023

Abstract
Purpose
This study aimed to investigate the frequency of COVID-19 vaccine-induced reactive change and potential factors correlated with increased FDG uptake on positron emission tomography (PET)/computed tomography (CT).
Materials and methods
We evaluated 285 patients who underwent PET/CT between June and September 2021 and had a known history of COVID-19 vaccination. Information on the injection site, vaccine type, and adverse reactions was obtained. We visually assessed the presence or absence of accumulation in the axillary and supraclavicular lymph nodes and the deltoid muscles. We measured the maximum standardized uptake value (SUVmax) using semi-quantitative analysis.
Results
Our study included 158 males and 127 females aged 16 to 94 years. The median time between vaccination and PET/CT was nine and 42 days for patients who had received their first and second doses, respectively. We observed axillary lymph node accumulation, supraclavicular lymph node accumulation, and deltoid muscle accumulation in 99 (SUVmax 1.07–25.1), nine (SUVmax 2.28–14.5), and 33 cases (SUVmax 0.93–7.42), respectively. In cases with axillary lymph node (P = 0.0066) or deltoid muscle (P = 0.047) accumulation, the shorter the time since vaccination, the higher the FDG accumulation. Patients with axillary lymph node accumulation were significantly younger (P < 0.0001) and had a significantly higher frequency of adverse reactions such as fever (P < 0.0001) and myalgia (P = 0.001). Logistic regression analysis also showed that age, sex, days since vaccination, and adverse reactions such as fever and myalgia were important factors for axillary lymph node accumulation.
Conclusion
Our study found that FDG accumulation in the axillary lymph nodes and deltoid muscle was higher within a shorter time after vaccination, and axillary lymph node accumulation was higher in young patients, females, and those with adverse reactions of fever and myalgia. Confirming the vaccination status, time since vaccination, and the presence of adverse reactions before PET may reduce false positives.
Exploring pineapple peel hydrolysate as a sustainable carbon source for xylitol production
Authors: Nasoha Nur Zahidah , Luthfi Abdullah Amru Indera , Roslan Muhammad Faizuddin , Hariz Hikmah Bajunaid , Bukhari Nurul Adela , Manaf Shareena Fairuz Abdul 
Published: August 2023

Abstract
This study explores utilizing pineapple peel (PP) hydrolysate as a promising carbon source for xylitol production, covering scopes from the pre-treatment to the fermentation process. The highest xylose concentration achieved was around 20 g/L via mild acid hydrolysis (5% nitric acid, 105°C, 20-minute residence time). Two sets fermentability experiments were carried out of varying pH levels in synthetic media that includes acetic acid as the main inhibitors and hydrolysate supplemented with diverse nitrogen source. The results revealed that pH 7 exhibited the highest xylitol production, yielding 0.35 g/g. Furthermore, urea was found to be a highly promising and cost-effective substitute for yeast extract, as it yielded a comparable xylitol production of 0.31 g/g with marginal difference of only 0.01g/g compared to yeast extract further highlights the viability of urea as the preferred option for reducing xylitol production cost. The absence of a significant difference between the synthetic media and hydrolysate, with only a marginal variance of 0.35 to 0.32 g/g, implies that acetic acid is indeed the primary constraint in xylitol production using PP hydrolysate. The study sheds light on PP biomass's potential for xylitol production, aligning economic benefits with environmental sustainability and waste management.
Construction of an index system of the biosafety incident response capability for nursing staff: A Delphi study
Authors: Wu Chao , Zhang Yinjuan , Zhang Xinyan , Fu Mimi , Zhang Hongli , Wu Jing , Li Lu , Chen Nana , Guo Xiaolan , Lang Hongjuan 
Published: August 2023

Abstract

Aim: This study was to establish an index system of the biosafety incident response capability for nursing staff.
Background: Faced with austere situations of the biosafety threat, the study on the requirement for nursing staff to respond to the biosafety incidents is not clear.
Methods. A modified recommendation for the Conducting and Reporting of Delphi studies was used to guide this study. After two-round Delphi survey, 28 nursing specialists from 6 provinces and cities around China, with expertise in three prevention research, epidemiology, military health service and biosafety incident rescue, established the index system of the biosafety incident response capability for nursing staff.
Results: The index system of the biosafety incident response capability for nursing staff following the two-round Delphi survey is composed of 4 primary indicators, namely, biosafety incident preparedness, monitoring capability, protection ability and nursing disposal capabilities, 10 secondary indicators and 49 tertiary indicators. The authority coefficient, judgment coefficient and familiarity degree of Delphi experts were 0.877, 0.911 and 0.843 respectively.
Conclusions: The index system of the biosafety incident response capability for nursing staff is scientific and reliable. It is helpful for nursing staff to perform better in biosafety incident by clarifying the specific items of biosafety response capability.
Relevance to clinical practice: Nursing staff, who are part of clinical workers, play an important role in maintaining biosafety. They must make clear how to manage the pertinent instances in light of the growing biosafety concern. The nursing staff can use the index system of the biosafety incident response capacity as a training resource to enhance their preparedness for the response, monitoring and early warning, protection and sensitivity management, and nursing disposal capabilities.
On-chip Raman spectroscopy of live single cells for the staging of oesophageal adenocarcinoma progression
Authors: Farooq Alisha , Wood Christopher D. , Ladbury John E. , Evans Stephen D. 
Published: August 2023

Abstract
The absence of early diagnosis contributes to oesophageal cancer being the sixth most common cause of global cancer-associated deaths, with a five-year survival rate of < 20%. Barrett’s oesophagus (BO) is the main pre-cancerous condition to adenocarcinoma development, characterised by the morphological transition of oesophageal squamous epithelium to metaplastic columnar epithelium. Early tracking and treatment of oesophageal adenocarcinoma could dramatically improve with diagnosis and monitoring of patients with BO. Current diagnostic methods involve invasive techniques such as endoscopies, and with only a few identified biomarkers of disease progression, the detection of oesophageal adenocarcinoma (OAC) is costly and challenging. In this work, single-cell Raman spectroscopy was combined with microfluidic techniques to characterise the development of oesophageal adenocarcinoma through the progression of healthy epithelial, Barrett’s oesophagus and oesophageal adenocarcinoma cell lines. Principal component analysis and linear discriminant analysis were used to classify the different stages of cancer progression. with the ability to differentiate between healthy and cancerous cells with an accuracy of 97%. Whilst the approach could also separate the diysplastic stages from healthy or cancer with high accuracy – the intra-class separation was approximately 68%. Overall, these results highlight the potential for rapid and reliable diagnostic/prognostic screening of BO patients.
Grafting of cotton fabrics with diallyldimethylammonium chloride
Authors: Kusuktham Miss Boonsri 
Published: August 2023

Abstract
The grafting of cotton fabrics with diallyldimethylammonium chloride was studied. The objective was to improve reactive dyes adsorption and antibacterial properties. The cotton fabric was modified with the diallyldimethylammonium chloride at room temperature using the redox initiator system of potassium persulfate as an initiator and N,N,N/,N/-tetramethylethylenediamine as an accelerator. It was found that the nitrogen content of the cationized cotton fabrics increased with an increase in the diallyldimethylammonium chloride concentration and stayed constant at a concentration of 40% (v/v). The adsorption of the reactive dyes on the cotton fabrics was investigated. The contact time of the reactive dyes adsorption on the modified cotton fabrics reached equilibrium at 30 minutes. The dye adsorption properties of the modified cottons depended on the concentration of the diallyldimethylammonium chloride, dyes concentration, chemical structure, and molecular weight of the reactive dyes. By grafting the fabrics with the diallyldimethylammonium chloride, the cotton fabric's antibacterial function was also enhanced.
Understanding flood plain aquaculture practice: An evidence from Daudkandi, Cumilla District, Bangladesh
Authors: Jareen Nadia Afreen , Imran Md. Faisal , Habib AHM Shafiullah , Naser Md. Niamul , Hossain Md. Nuralam 
Published: August 2023

Abstract
The floodplains of Bangladesh represent a vital common-pool resource, and the aquaculture sector stands alone as one of the most prosperous industries in the country. This industry is essential for the provision of food and nutrition, employment opportunities, and earnings in foreign currencies for a country's development and economic growth. This study examines the prevailing fish culture practices, assemblage and growth patterns, marketing strategies, profit-sharing mechanisms, costs and benefits, community-based approaches, management protocols, poaching activities, and the current administrative setup at the government level. This research was conducted at "Shapla Fisheries", a floodplain fish culture venture at Raipur Village, in Daudkandi Upazila, Cumilla. This study applied the participatory rural appraisal (PRA) approach to understand the floodplain's intensive and nonintensive culture practices. The survey identified 39 fish species in the study area, with 5% endangered, 10% nearly threatened, 8% vulnerable, 54% least concerned, and 23% unlisted according to Red List 2015 criteria. Under rigorous culture, Barbonymus gonionotus reached 4566.67% growth. In 2018, the proportion of induced species in the intense system was 79.63%, representing a significant increase from the 60.18% observed in 2008. Intensive aquaculture systems boost earnings and have improved profits but lost biological biodiversity. This study demonstrates that small indigenous species (SIS) maintain a healthy ecology despite intensive farming focused on financial growth. Thus, the current floodplain aquaculture system lacks community association and guaranteed earnings. This study proposed community-based native aquaculture practices to protect wild species, conserve small indigenous species (SIS), and support floodplain-dependent populations.
LncRNA MLLT4-AS1 induces autophagy to inhibit tumorigenesis of cervical cancer through modulating the myosin-9/ATG14 axis
Authors: zhang Tingting , Ji tiantian , Xue Yuanyuan , Duan Zhao , Fan Yinxia 
Published: August 2023

Abstract
The regulatory mechanism of long non-coding RNAs (lncRNAs) in autophagy is as yet not well established. In this research, we show that the lncRNA MLLT4-AS1 is induced by the MTORC inhibitor PP242 and rapamycin in cervical cells. Overexpression of MLLT4-AS1 promotes autophagy and inhibits tumorigenesis and the migration of cervical cancer cells, whereas knockdown of MLLT4-AS1 attenuates PP242-induced autophagy. Mass spectrometry, RNA fluorescence in situ hybridization (RNA-FISH), and immunoprecipitation assays were performed to identify the direct interactions between MLLT4-AS1 and other associated targets, such as myosin-9 and autophagy-related 14(ATG14). MLLT4-AS1 was upregulated by H3K27ac modification with PP242 treatment, and knockdown of MLLT4-AS1 reversed autophagy by modulating ATG14 expression. Mechanically, MLLT4-AS1 was associated with the myosin-9 protein, which further promoted the transcription activity of the ATG14 gene. In conclusion, we demonstrated that MLLT4-AS1 acts as a potential tumor suppressor in cervical cancer by inducing autophagy, and H3K27ac modification–induced upregulation of MLLT4-AS1 could cause autophagy by associating with myosin-9 and promoting ATG14 transcription.
Analysis of Factors Associated with Undescended Testis in Patients with Congenital Diaphragmatic Hernia
Authors: Umeda Satoshi , Yoshida Masayuki , Matsumoto Sayaka , Sakai Takaaki , Noguchi Yuki , Matsuura Rei , Zenitani Masahiro , Usui Noriaki 
Published: August 2023

Abstract
Purpose
This study aimed to investigate the incidence and clinical factors associated with undescended testes (UDT) in patients with congenital diaphragmatic hernia (CDH).
Methods
We retrospectively reviewed the incidence of UDT in male neonates admitted to our institution and underwent surgery for CDH between January 2006 and December 2022. Patients were divided into two groups based on the presence or absence of UDT, and risk factors for UDT were compared between the two groups.
Results
Among the 66 male neonates with CDH, 16 (24.2%) developed UDT. Patients with UDT had a significantly smaller gestational age (p = 0.026), lower birth weight (p = 0.042), and lower Apgar score at 1 minute (p = 0.016) than those without UDT. They had a significantly higher incidence of large diaphragmatic defects (p = 0.005), received more patch closures (p = 0.020), had a longer mechanical ventilation period (p = 0.034), and longer hospital stay (p = 0.028).
Conclusion
Our data indicate a strong correlation between CDH and UDT. In patients with CDH, the incidence of UDT was related not only to patients’ prematurity but also to the large diaphragmatic defect, suggesting that an insufficient increase in intra-abdominal pressure during the foetal period may result in the development of UDT in patients with CDH.
Effect of bilateral low serratus anterior plane block on quality of recovery after trans-subxiphoid robotic thymectomy: Results of a randomized placebo-controlled trial
Authors: Fu Yu , Fu Huimin , Liu Huqing , Wen Zongmei , Lv Xin , Lu Yugang 
Published: August 2023

Abstract
Scientific literature about pain management for patients undergoing trans-subxiphoid robotic thymectomy (TRT) remains limited. This randomized placebo-controlled trial aimed to investigate the impact of ultrasound-guided, bilateral, low level (T8-T9) deep serratus anterior plane (DSAP) blocks on postoperative recovery quality and postoperative analgesia in patients undergoing TRT. 39 patients undergoing TRT were randomized to receive either low DSAP blocks (T8-T9) with 20 ml 0.375% ropivacaine under general anesthesia (Group S) or the sham block with 0.9% normal saline (Group C) on each side. The primary outcome was the global Quality of Recovery-40 (QoR-40) score at 24 hours after surgery. Secondary outcomes included numeric rating scale (NRS) scores over time, postoperative 48 hours opioid consumption, QoR-40 scores at 48 hours, 30 days and 90 days after surgery. The QoR-40 scores on POD1-2 were higher among Group S compared with those in Group C [179.1 (4.9) vs 167.7 (2.8), P < 0.01; 187.7 (4.6) vs 178.1 (3), P < 0.01, respectively]. Pain scores were significantly lower in Group S than in Group C, both during resting and motion at postoperative 6h, 12h, and 24h (P < 0.05 for each). The total amount of sufentanil consumed in the first 48 h was lower in Group S than in Group C [61.4 (4.9) vs 78.9 (4.6), P < 0.001]. The bilateral low DSAP blocks with ropivacaine enhanced the quality of recovery for 2 days postoperatively, relieved postsurgical pain, and reduced opioid consumption during the early postoperative period in patients undergoing TRT.
Assessment of Respiratory Mechanics in Patients With Covid- 19 Acute Respiratory Distress Syndrome (ARDS): Are There Mortality Impacts?
Authors: Gutiérrez Luis , Araya Karina , Becerra Mara , Pérez Camilo , Valenzuela Jorge , Lera Lydia , Lizana Pablo A. , Sol Mariano , Muñoz-Cofré Rodrigo 
Published: August 2023

Abstract
Background
The 2019 coronavirus (COVID-19) can generate acute respiratory distress syndrome (ARDS), requiring advanced management within the Intensive Care Unit (ICU) using invasive mechanical ventilation (IMV). However, managing this phenomenon has seen learning and improvements through direct experience. This study aimed to describe the assessment of IMV variables for ARDS patients following COVID-19 hospitalization in the ICU, and their relation with mortality.
Methods
Observational and retrospective study. The sample was divided into two, the surviving group (SG) and the non-surviving group (NSG). Clinical data were extracted from the electronic clinical file and the respiratory therapist record sheet. The following information was obtained: Patient medical history: gender, age, co-morbidities, arterial gases, days on IMV, and IMV parameters. Cox proportional regression models for 2–68 days of mortality were estimated to analyze adjusted hazard ratios (HR) by the presence of multimorbidity and ventilation reduction.
Results
Out of a total of 101 patients, the total mortality was 32%. There was a significant rise in respiratory rate (RR) (29.12 ± 4.24–26.78 ± 3.59, p = 0.006), Driving pressure (DP) (11.33 ± 2.39–9.67 ± 1.84, p = 0.002), Ventilatory rate (VR) (2.26 ± 0.66–1.89 ± 0.45, p = 0.001) and the partial pressure of carbon dioxide in arterial blood (PCO2)(45.91 ± 8.59–41.90 ± 6.4, p = 0.001) and a significant decrease in Static compliance (Cest) (35.49 ± 8.64–41.45 ± 9.62, p = 0.003) and relation between Arterial oxygen pressure/Inspirated oxygen fraction (PaO2/FiO2) (201.5 ± 53.98–227.8 ± 52.11, p = 0.008) after 72 hours of IMV, within the NSG compared to the SG. Apart from these points, multi-morbidity (HR = 3.208, p = 0.010) and DP (HR = 1.228, p = 0.030) and VR variables (HR = 2.267, p = 0.027) had more death probabilities.
Conclusion
The results of this study indicate that there was a significant increase in FR, DP, VR, and CO2 and a significant drop in Cest and PaO2/FiO2 among the NSG compared with the SG. Apart from this, the DP and VR variables, multi-morbidity and being male. have more possibility of death.
A systematic review and meta-analysis of the effectiveness of experimental techniques in altering intrusive memories of lab-analogue trauma
Authors: Hu Xiaoqing , Varma Mohith , Zeng Shengzi , Singh Laura , Holmes Emily , Huang Jingyun 
Published: August 2023

Abstract

Experiencing trauma can lead to intrusive memories, a hallmark symptom of post-traumatic stress disorder and transdiagnostic feature of stress-related disorders. Understanding why intrusions increase or decrease is pivotal in developing effective interventions to safeguard mental wellness following trauma. Building on lab-analogue trauma paradigms, we conducted a pre-registered (PROSPERO: CRD42021224835)meta-analysis to investigate the effectiveness of experimental techniques in either reducing or increasing intrusion frequency (e.g., assessed with a daily diary), intrusion-related distress and symptoms. Aggregating data from 126 articles examining 118 techniques with 584 effect sizes and 11,132 participants, we found that techniques hypothesized to reduce intrusion frequency significantly decreased intrusion frequencies, intrusion-related distress and symptoms (g = 0.11— 0.49). Moreover, techniques hypothesized to increase intrusions significantly increased intrusion frequencies and their associated distress (g = -0.15 — -0.19). Moderator and focal analyses showed that behavioral techniques tapping into imagery-based processing (e.g. playing Tetris following trauma reminder) were most effective in reducing intrusion frequency and symptoms (g = 0.22 — 0.68). However, those targeting verbal-based processing (e.g., rumination, adding contextual information) significantly increased intrusions, albeit to a small degree (g = -0.29 — -0.30). We further supplement the meta-analysis with a website that allows researchers to analyze the dataset to facilitate research synthesis and future endeavor. While techniques that reduce intrusions can inform the development of evidence-based interventions to reduce intrusions in real life, techniques that may (inadvertently) increase intrusions underscore vulnerabilities factors and thus can inform preventive measures.