bluuebunny/crossref_metadata_2025_split huggingface dataset:
Basolateral amygdala circuits supporting negative emotional bias in a mouse model for depression
Authors: Bigot Mathilde , De Badts Claire-Hélène , Benchetrit Axel , Vicq Éléonore , Moigneu Carine , Meyrel Manon , Wagner Sébastien , Houenou Josselin , Lledo Pierre-Marie , Henry Chantal , Alonso Mariana 
Published: January 2023

AbstractNegative emotional bias is an essential hallmark of depression reflected by negative shift in hedonic valence assignment to emotional stimuli. Pleasant cues become less attractive and unpleasant ones more aversive. Given the crucial role of amygdala in valence coding, we hypothesize that specific basolateral amygdala (BLA) circuits alterations might support negative emotional bias associated with depressive states.Using a translational assay, we evaluate odor valence assignment in an animal model for depression chronically administered by corticosterone (CORT). We show spontaneous negative bias in depressive-like mice that attribute more negative valences for both attractive and aversive odors, mimicking thus the bias observed in depressed bipolar patients.Combining CTB and rabies-based tracing withex vivomeasurements of neuronal activity and chemogenetics experiments, we find that the CORT treatment reduces BLA-to-nucleus accumbens (NAc) neuronal activity and increases BLA-to-central amygdala activity, circuits respectively known to be involved in positive and negative valence encoding. Alterations in presynaptic connectivity of BLA-projecting neurons accompany these activity shifts. Interestingly, inputs from the paraventricular thalamus nucleus (PVT) towards BLA-to-NAc neurons are reduced in CORT-treated mice. Finally, chemogenetically activating the BLA-to-NAc circuit attenuates the negative bias in CORT-treated mice as well as the depressive-like phenotype, similarly than Fluoxetine antidepressant treatment. Altogether, we demonstrate that depressive states are associated with negative emotional bias both in human and mice. This bias is supported by activity shifts of specific BLA circuits along with durable presynaptic connectivity changes, but it could be alleviated by antidepressant drug or activity manipulation of altered BLA circuit.
Eye movements track prioritized auditory features in selective attention to natural speech
Authors: Gehmacher Quirin , Schubert Juliane , Schmidt Fabian , Hartmann Thomas , Reisinger Patrick , Rösch Sebastian , Schwarz Konrad , Popov Tzvetan , Chait Maria , Weisz Nathan 
Published: January 2023

AbstractOver the last decades, cognitive neuroscience has identified a distributed set of brain regions that are critical for attention - one of the key principles of adaptive behavior. A strong anatomical overlap with brain regions critical for oculomotor processes suggests a joint network for attention and eye movements. However, the role of this shared network in complex, naturalistic environments remains understudied. Here, we investigated eye movements in relation to (un)attended sentences of natural speech in simultaneously recorded eye tracking and magnetoencephalographic (MEG) data. Using temporal response functions (TRF), we show that eye gaze tracks acoustic features (envelope and acoustic onsets) of attended speech, a phenomenon we termedocular speech tracking. Ocular speech envelope tracking even differentiates a target from a distractor in a multi speaker context and is further related to intelligibility. Moreover, we provide evidence for its contribution to neural differences in speech processing, emphasizing the necessity to consider oculomotor activity in future research and in the interpretation of neural differences in auditory cognition. Our results extend previous findings of a joint network of attention and eye movement control as well as motor theories of speech. They provide valuable new directions for research into the neurobiological mechanisms of the phenomenon, its dependence on learning and plasticity, and its functional implications in social communication.
Identification of Candidate Mitochondrial Inheritance Determinants Using the Mammalian Cell-Free System
Authors: Zuidema Dalen , Jones Alexis , Song Won-Hee , Zigo Michal , Sutovsky Peter 
Published: January 2023

AbstractThe degradation of sperm-borne mitochondria after fertilization is a conserved event. This process known as post-fertilization sperm mitophagy, ensures exclusively maternal inheritance of the mitochondria-harbored mitochondrial DNA genome. This mitochondrial degradation is in part carried out by the ubiquitin-proteasome system. In mammals, ubiquitin-binding pro-autophagic receptors such as SQSTM1 and GABARAP have also been shown to contribute to sperm mitophagy. These systems work in concert to ensure the timely degradation of the sperm-borne mitochondria after fertilization. We hypothesize that other receptors, cofactors, and substrates are involved in post-fertilization mitophagy. Mass spectrometry was used in conjunction with a porcine cell-free system to identify other autophagic cofactors involved in post-fertilization sperm mitophagy. This porcine cell-free system is able to recapitulate early fertilization proteomic interactions. Altogether, 185 proteins were identified as statistically different between control and cell-free treated spermatozoa. Six of these proteins were further investigated, including MVP, PSMG2, PSMA3, FUNDC2, SAMM50, and BAG5. These proteins were phenotyped using porcinein vitrofertilization, cell imaging, proteomics, and the porcine cell-free system. The present data confirms the involvement of known mitophagy determinants in the regulation of mitochondrial inheritance and provides a master list of candidate mitophagy co-factors to validate in the future hypothesis-driven studies.
Compressed phenotypic screens for complex multicellular models and high-content assays
Authors: Mead Benjamin E. , Kummerlowe Conner , Liu Nuo , Kattan Walaa E. , Cheng Thomas , Cheah Jaime H. , Soule Christian K. , Peters Josh , Lowder Kristen E. , Blainey Paul C. , Hahn William C. , Cleary Brian , Bryson Bryan , Winter Peter S. , Raghavan Srivatsan , Shalek Alex K. 
Published: January 2023

AbstractHigh-throughput phenotypic screens leveraging biochemical perturbations, high-content readouts, and complex multicellular models could advance therapeutic discovery yet remain constrained by limitations of scale. To address this, we establish a method for compressing screens by pooling perturbations followed by computational deconvolution. Conducting controlled benchmarks with a highly bioactive small molecule library and a high-content imaging readout, we demonstrate increased efficiency for compressed experimental designs compared to conventional approaches. To prove generalizability, we apply compressed screening to examine transcriptional responses of patient-derived pancreatic cancer organoids to a library of tumor-microenvironment (TME)-nominated recombinant protein ligands. Using single-cell RNA-seq as a readout, we uncover reproducible phenotypic shifts induced by ligands that correlate with clinical features in larger datasets and are distinct from reference signatures available in public databases. In sum, our approach enables phenotypic screens that interrogate complex multicellular models with rich phenotypic readouts to advance translatable drug discovery as well as basic biology.
Telomerase-independent survival leads to a mosaic of complex subtelomere rearrangements inChlamydomonas reinhardtii
Authors: Chaux Frédéric , Agier Nicolas , Garrido Clotilde , Fischer Gilles , Eberhard Stephan , Xu Zhou 
Published: January 2023

AbstractTelomeres and subtelomeres, the genomic regions located at chromosome extremities, are essential for genome stability in eukaryotes. In the absence of the canonical maintenance mechanism provided by telomerase, telomere shortening induces genome instability. The landscape of the ensuing genome rearrangements is not accessible by short-read sequencing. Here, we leverage Oxford Nanopore Technologies long-read sequencing to survey the extensive repertoire of genome rearrangements in telomerase mutants of the model green microalgaChlamydomonas reinhardtii. In telomerase mutant strains grown for ∼700 generations, most chromosome extremities were capped by short telomere sequences that were either recruitedde novofrom other loci or maintained in a telomerase-independent manner. Other extremities did not end with telomeres but only with repeated subtelomeric sequences. The subtelomeric elements, including rDNA, were massively rearranged and involved in breakage-fusion-bridge cycles, translocations, recombinations and chromosome circularization. These events were established progressively over time and displayed heterogeneity at the subpopulation level. New telomere-capped extremities composed of sequences originating from more internal genomic regions were associated with high DNA methylation, suggesting thatde novoheterochromatin formation contributes to restore chromosome end stability inC. reinhardtii. The diversity of alternative strategies to maintain chromosome integrity and the variety of rearrangements found in telomerase mutants are remarkable and illustrate genome plasticity at short timescales.
Compressed Perturb-seq: highly efficient screens for regulatory circuits using random composite perturbations
Authors: Yao Douglas , Binan Loic , Bezney Jon , Simonton Brooke , Freedman Jahanara , Frangieh Chris J. , Dey Kushal , Geiger-Schuller Kathryn , Eraslan Basak , Gusev Alexander , Regev Aviv , Cleary Brian 
Published: January 2023

AbstractPooled CRISPR screens with single-cell RNA-seq readout (Perturb-seq) have emerged as a key technique in functional genomics, but are limited in scale by cost and combinatorial complexity. Here, we reimagine Perturb-seq’s design through the lens of algorithms applied to random, low-dimensional observations. We present compressed Perturb-seq, which measures multiple random perturbations per cell or multiple cells per droplet and computationally decompresses these measurements by leveraging the sparse structure of regulatory circuits. Applied to 598 genes in the immune response to bacterial lipopolysaccharide, compressed Perturb-seq achieves the same accuracy as conventional Perturb-seq at 4 to 20-fold reduced cost, with greater power to learn genetic interactions. We identify known and novel regulators of immune responses and uncover evolutionarily constrained genes with downstream targets enriched for immune disease heritability, including many missed by existing GWAS or trans-eQTL studies. Our framework enables new scales of interrogation for a foundational method in functional genomics.
Investigation on intergranular corrosion susceptibility of a nitrogen-containing austenitic stainless steel QN2109 by optimized DL-EPR tests
Authors: Wang Xiangyu , He Pei , Zhou Qinglong , Zhou Qingyuan , Cheng Manlang , Sun Yangting , Jiang Yiming , Jiang Laizhu , Li Jin 
Published: January 2023

Purpose
The purpose of this study is to investigate the intergranular corrosion (IGC) susceptibility of a nitrogen-containing austenitic stainless steel QN2109. The intergranular corrosion (IGC) susceptibility of a nitrogen-containing austenitic stainless steel QN2109 was investigated.

Design/methodology/approach
The double-loop electrochemical potentiodynamic reactivation (DL-EPR) tests were carried out. Scanning electron microscopy and atomic force microscopy were used to characterize the microstructure.

Findings
The optimized test condition for QN2109 was 1 M H2SO4 + 0.01 M NH4SCN at 40°C. The nose temperature of the temperature–time–sensitization (TTS) curve of QN2109 plot was approximately 750°C. Moreover, the IGC susceptibility started to appear at approximately 120 min. The Cr-depletion zone of QN2109 was generated by the formation of M23C6 rather than by the addition of nitrogen. The depth–width ratio of the grain boundaries after the DL-EPR tests decreased as the aging temperature increased. The degree of Cr depletion and size of the Cr-depletion zone at the grain boundary were reflected by the degree of sensitization and depth–width ratio, respectively.

Originality/value
The optimized test condition for DL-EPR tests of a nitrogen-containing austenitic stainless steel QN2109 was investigated. The TTS curve of QN2109 was first plotted to avoid IGC failure. The morphology of the Cr-depletion zone was reflected by the depth–width ratio.
Food safety and convenience meals: consumers' actual and perceived behaviours – a mixed methods study
Authors: Lavelle Fiona , McKernan Claire , Murphy Blain , Dean Moira 
Published: January 2023

PurposeFoodborne disease remains a significant public health threat and cause for concern. Despite numerous studies indicating that the domestic kitchen is a high-risk area for foodborne illness, consumers believe it is low-risk. The type of food being prepared in the kitchen has drastically changed, with an increased use of convenience products, perceived low-risk products. Little is known about consumers' behaviours with convenience meals. Therefore, it is essential to understand actual and perceived food safety behaviours in their use.MethodologyA mixed-methods approach using in-home observations and semi-structured interviews was undertaken with participants from the UK and the Republic of Ireland (N = 50). Observational data was summarized using descriptive statistics and an inductive thematic analysis was conducted to interpret the qualitative data.FindingsIn this study general food safety behaviours, such as handwashing, use-by date checking, as well as identifying obvious safety hazard were suboptimal. However, participants' convenience meal safety behaviours were as optimal as possible with the provided instructions. The qualitative data supported the participants' struggle with the provided on-packet instructions. They also displayed participants' uncertain perceptions around different products, for example if oven-products could be reheated multiple times, and varying levels of concern around meat and dairy products. Consumers urged for more detailed and clearer instructions with these products. Recommendations for producers are provided to enhance consumer experience with the use of the products as well as contributing towards ensuring consumer safety.Originality/valueMixed-methods approach investigating consumers' real and perceived food safety behaviours.
Numerical and experimental investigation for enhancing thermal performance of a concentric heat exchanger using different scenarios
Authors: Aytaç İpek , Badali Yosef , Tuncer Azim Doğuş 
Published: January 2023

Purpose
Heat exchangers (HEs) which provide heat transfer and transfer energy through direct or indirect contact between fluids have an essential role in many processes as a part of various industries from pharmaceutical production to electronic devices. Using nanofluid as working fluid and integrating different types of turbulators could be used to upgrade the thermal effectiveness of HEs. Recently, to obtain more increment in thermal effectiveness, hybrid nanofluids are used that are prepared by mixing two or more various nanoparticles. The purpose of this experimental and numerical study is investigating different scenarios for improving the effectiveness of a concentric U-tube type HE.

Design/methodology/approach
In the numerical section of this study, different turbulator modifications, including circular and quarter circular rings, were modeled to determine the effect of adding turbulator on thermal performance. In addition, Al2O3/water and SiO2/water single and Al2O3–SiO2/water hybrid nanofluids were experimentally tested in an unmodified concentric U-tube HE in two different modes, including counter flow and parallel flow. Al2O3–SiO2/water hybrid nanofluid was prepared at 2% (wt./wt.) particle ratio and compared with Al2O3/water and SiO2/water single type nanofluids at same particle ratios and with distilled water.

Findings
Numerical modeling findings exhibited that integrating turbulators to the concentric tube type HE caused to raise in the effectiveness by improving heat transfer area. Also, experimental results indicated that using both hybrid and single type nanofluids notably upgraded the thermal performance of the concentric U-tube HE. Integrating turbulators cannot be an effective alternative in a concentric U-tube type HE with lower diameter because of raise in pressure drop. Numerically achieved findings exhibited that using quarter circular turbulators decreased pressure drop in comparison with circular turbulators. According to the experimental outcomes, using hybrid Al2O3–SiO2/water nanofluid leads to obtain more thermal performance in comparison with single type nanofluids. The highest increment in overall heat transfer coefficient of HE by using Al2O3–SiO2/water nanofluid achieved as 58.97% experimentally.

Originality/value
The overall outcomes of the current research exhibited the positive impacts of using hybrid nanofluid and integrating turbulators. In this empirical and numerical survey, numerical simulations were performed to specify the impact of applying different turbulators and hybrid nanofluid on the flow and thermal characteristics in a concentric U-tube HE. The achieved outcomes exhibited that using hybrid nanofluid can notably increase the thermal performance with negligible pressure drop in comparison with two different turbulator modifications.
Sustainable maintenance planning in the petroleum industry: an application of system dynamics approach
Authors: Hosseinzadeh Mahnaz , Samadi Foroushani Marzieh , Ghayem Hakim , Mehregan Mohammad Reza 
Published: January 2023

PurposeWhile the petroleum industry remains to be the main source of energy in the world, it is responsible for a large amount of resource consumption, environmental emission and safety issues. In this industry, most of the refinery equipment are running out of their designed life cycle, leading to many challenges regarding equipment reliability, products quality, organizations’ profitability, human resources safety and job satisfaction, and environmental pollution, which affects not only the human resources of the refinery but also the people who live in the vicinity. This study aims to model and simulate the maintenance system of an oil refinery to reduce the rotating equipment’s downtime while simultaneously considering the three pillars of sustainability.Design/methodology/approachConsidering the complexity of the system and its inherent dynamism, System Dynamics (SD) approach is applied to model and simulate the maintenance system of an oil refinery, aiming at reducing equipment’s downtime considering the three pillars of sustainability simultaneously. As a case study, the maintenance system of rotating equipment in the Abadan oil refinery of Iran is investigated.FindingsIndividual policies are investigated and categorized into three main groups: economic, social and environmental. The dynamic nature of the system demonstrates that applying combinations of the policies would be more effective than performing individual ones or even a combination of all policies at the same time. The findings show that to manage the maintenance and reliability issues in complex industries, only operational level maintenance strategies would not be helpful; rather, a holistic strategic solution counting different suppliers or even the government policies supporting the operational level maintenance decisions would be effective.Originality/valueThis study is the first which brings the perspective of sustainable policy-making in the SD modeling of a complex maintenance system like that of the petroleum industry. The developed model considers economic, environmental and social objectives simultaneously. Besides, it reflects the role of different stakeholders in the system. Furthermore, the policy-making attempt is not limited to the operational level corrective and maintenance solutions; instead, a comprehensive, holistic view is applied.
Dysfunctional behaviour in university accounting schools: a tale of management control
Authors: Tucker Basil , Parker Lee D. , Scully Glennda E.M. 
Published: January 2023

Purpose
The purpose of this inductive, exploratory study is to provide foundational insights into the role of management control in dealing with dysfunctional behaviour within accounting schools in changing environment of Australian universities.

Design/methodology/approach
Evidence is drawn from semi-structured interviews with 28 current or previous heads of school, research deans, deans of teaching and learning, school managers and human resource managers from 16 Australian universities and interpreted from the theoretical perspective of rational choice theory.

Findings
The findings suggest the incidence of a range of dysfunctional behaviours occurring in accounting schools. Even when such behaviours are limited in frequency, their consequences are nevertheless found to have far-ranging and potentially destructive change impacts for both individuals and the university. Formal management control systems designed to address such behaviours are perceived to be largely ineffective in identifying, managing, eliminating or even mitigating the consequences of such dysfunctionality. Instead, it is informal control processes that are preferred in dealing with dysfunctionality.

Originality/value
This study enhances our understanding of the role of management control in dealing with dysfunctional behaviour within university accounting schools, and points not only to the difference between the design and use of management controls but also to the implications of this disconnect between the underlying intent of control design and their actual use in the context of environments that are subject to significant change.
The “snowflakes” of modern society: a qualitative investigation of female university students' anxiety about adulting
Authors: Talbot Amelia , O'Reilly Michelle , Dogra Nisha 
Published: January 2023

PurposeThe paper aims to explore the anxiety of university students. The authors note that the rhetoric of the snowflake is frequently invoked in lay discourse to characterise a generation of young people as overly sensitive. This misleading conceptualisation is potentially stigmatising.Design/methodology/approachInterviews were conducted with tweleve young women (18–25 years) about anxiety during their transition through university and into adulthood.FindingsThe authors identified three themes: (1) students in a modern world, (2) gendered demands and (3) anxiety of adulting. Analysis demonstrated numerous, transecting and discourse-informed anxieties about modern life.Practical implicationsUniversity professionals may benefit from understanding the gendered dimensions of anxiety associated with transitions to adulthood, including the increased pressures to succeed and achieve.Originality/valueThe arguably pejorative label of “snowflake” could negatively impact the social progress made in recognising the importance of taking care of mental health and help-seeking. This is especially concerning for females, as they have higher prevalence of anxiety conditions than males.