bluuebunny/crossref_metadata_2025_split huggingface dataset:
EFEKTIVITAS PENERAPAN E-TILANG DALAM PENINDAKAN PELANGGARAN LALU LINTAS DI WILAYAH HUKUM POLRESTABES SURABAYA
Authors: Nagendra Aldy Putu , Sushanty Vera Rimbawani 
Published: March 2023

Artikel ini bertujuan untuk meneliti efektivitas penerapan e-tilang khususnya di wilayah hukum Polrestabes Surabaya sejak diberlakukannya sampai dengan tahun 2020 berdasarkan sumber bahan hukum primer Undang-Undang Nomor 22 Tahun 2009 tentang Lalu Lintas Dan Angkutan Jalan (“UU LLAJ”) serta Peraturan Pemerintah Nomor 80 Tahun 2012 tentang Tata Cara Pemeriksaan Kendaraan Bermotor Di Jalan Dan Penindakan Pelanggaran Lalu Lintas Dan Angkutan Jalan (PP 80/2012), serta Peraturan Mahkamah Agung Republik Indonesia Nomor 12 Tahun 2016 Tentang Tata Cara Penyelesaian Perkara Pelanggaran Lalu Lintas dan peraturan pelaksana lainnya. Adapun fokus masalah ditujukan pada bagaimanakah penerapan sistem e-tilang dalam penyelesaian perkara tindak pidana pelanggaran lalu lintas dan efektivitas penerapan sistem e-tilang dalam pencegahan tindak pidana pelanggaran lalu lintas di wilayah hukum Kepolisian Daerah Jawa Timur. Metode yang digunakan pada penelitian ini adalah yuridis empiris melalui pengumpulan sumber data dan peraturan melalui wawancara dan kemudian diolah untuk mendapatkan kesimpulan hasil penelitian. Kajian ini menyimpulkan bahwa penerapan sistem e-tilang terhadap penyelesaian perkara pelanggaran lalu lintas di wilayah hukum Polresta Surabaya telah cukup efektif sesuai dengan tujuan yang diharapkan dengan dilihat dari dampak turunnya jumlah pelanggaran lalu lintas selama tahun bersangkutan sebagaimana dalam periode penelitian yang dilakukan..

Kata Kunci: e-Tilang, Efektivitas, Polrestabes Surabaya.
PERAN SATUAN POLISI PAMONG PRAJA DALAM PENEGAKAN PERATURAN DAERAH DI KABUPATEN SUKABUMI
Authors: Junaedi Gatot Sambas 
Published: March 2023

Tugas Satpol PP secara umum adalah sebagai Penegak Perda dan Perkada, Penyelenggara Trantibumtranmas, serta melaksanakan pelindungan masyarakat, namun realita yang terjadi di lapangan peran dari satpol pp tersebut belum diketahui secara luas oleh masyarakat secara umum. Sehubungan hal tersebut maka Penelitian ini bertujuan untuk mengetahui sedalam apa peran satpol pp kabupaten sukabumi dalam menegakan Perda dan Perkada serta dalam pelaksanaan trantibumtranmas. Methode penelitian disini menggunakan methode penelitian kualitatif. Setiap Data didapatkan melalui wawancara, observasi, dan studi literatur dengan Teknik analytical datanya yaitu pengumpulan, reduksi, penyajian, verifikasi dan kesimpulan data. Hasil dari penelitian ini adalah bahwa Peran Satpol PP Kabupaten Sukabumi dalam melaksanaan tugas pokok dan fungsinya dalam rangka trantibumtranmas dan penegakan perda pun rutin dilaksanakan dengan terstruktur, sistemasis dan masif, dengan rincian giat diantaranya Pembinaan dan Penyuluhan Perda dan Perkada, Sidang Tipiring (Tindak Pidana Ringan) ditempat, Operasi Yustisi Penertiban Pedagang Kaki Lima (PKL) dan Tertib Kebersihan, Operasi Penertiban IMB, Operasi Pekat, Operasi Tertib Spanduk, Operasi Minuman Keras, Patroli di Wilayah Rawan Pelanggaran, Patroli Kewilayahan Siskamling dll. Adapun kesimpulan terkait Peran Satpol PP berdasarkan Tupoksinya yaitu Penyelenggara Ketertiban Umun dan Ketentraman Masyarakat, Menegakan Perda dan Pelindung Masyarakat sudah dilaksanakan oleh Satuan Polisi Pamong Praja kabupaten Sukabumi sudah Cukup Baik dimana jumlah pelanggaran Perda dari tahun ke tahun menurun serta trantibumtranmas pun berjalan dengan kondusif diwilayah kabupaten sukabumi.

Kata Kunci: Peranan, Satuan Polisi Pamong Paraja, Penegakan Peraturan Daerah.
Detection and identification of Mucorales and Aspergillus in paraffin-embedded samples by real-time quantitative PCR
Authors: Jiang Xiaolin , Jiang Yong , Ye Feng 
Published: March 2023

BackgroundIn this study, we used real-time quantitative PCR (RQ-PCR) to rapidly detect Mucorales and Aspergillus in formalin-fixed, paraffin-embedded (FFPE) samples, targeting 18SrRNA gene and 28SrRNA gene. Identification of Mucorales and Aspergillus was analysed by combining Mucorales RQ-PCR (Mucorales18SrRNA and Mucorales28SrRNA) with Aspergillus RQ-PCR (Aspergillus18SrRNA and Aspergillus28SrRNA).ObjectivesThe aims of this study were to compare the diagnostic performances of four RQ-PCR assays as single and combined diagnostic and identification tools.MethodsWe collected 12 control group samples and 81 experimental group samples diagnosed by histopathology, including mucormycosis (19 patients, 21 FFPE samples), aspergillosis (54 patients, 57 FFPE samples) and mucormycosis with aspergillosis (3 patients, 3 FFPE samples). All samples were detected by four RQ-PCR tests to compare and analyze diagnostic performance.ResultsThe sensitivities of Mucorales18SrRNA and Mucorales28SrRNA were both 75%, with the tests having specificities of 97.10% and 94.20%. The sensitivities of Aspergillus18SrRNA and Aspergillus28SrRNA were 73.33% and 65%, with the tests having specificities of 87.88% and 81.82%. The values of the evaluation indexes of the combined detection of Mucorales28SrRNA and Aspergillus18SrRNA (M28A18) were the highest with a kappa coefficient value of 0.353, followed by M18A18. M28A18 had a sensitivity of 67.90% and a specificity of 100%.ConclusionsWe recommend using the combination of Mucorales RQ-PCR and Aspergillus RQ-PCR as a screening tool to detect samples suspected of mucormycosis and/or aspergillosis.
BPOZ-2 is a negative regulator of the NLPR3 inflammasome contributing to SARS-CoV-2-induced hyperinflammation
Authors: Li Jingfei , Lin Haotian , Fan Tinghui , Huang Linfei , Zhang Xinyong , Tai Yanhong , Fang Yi , Li Qihong , Zhao Ruzhou , Wang Penghao , Zhou Li , Wan Luming , Wu Yuhua , Zhong Hui , Wei Congwen , Yang Xiaopan 
Published: March 2023

IntroductionInflammation play important roles in the initiation and progression of acute lung injury (ALI), acute respiratory distress syndrome (ARDS), septic shock, clotting dysfunction, or even death associated with SARS-CoV-2 infection. However, the pathogenic mechanisms underlying SARS-CoV-2-induced hyperinflammation are still largely unknown.MethodsThe animal model of septic shock and ALI was established after LPS intraperitoneal injection or intratracheal instillation. Bone marrow-derived macrophages (BMDMs) from WT and BPOZ-2 KO mouse strains were harvested from the femurs and tibias of mice. Immunohistology staining, ELISA assay, coimmunoprecipitation, and immunoblot analysis were used to detect the histopathological changes of lung tissues and the expression of inflammatory factors and protein interaction.Results and conclusionsWe show a distinct mechanism by which the SARS-CoV-2 N (SARS-2-N) protein targets Bood POZ-containing gene type 2 (BPOZ-2), a scaffold protein for the E3 ubiquitin ligase Cullin 3 that we identified as a negative regulator of inflammatory responses, to promote NLRP3 inflammasome activation. We first demonstrated that BPOZ-2 knockout (BPOZ-2 KO) mice were more susceptible to lipopolysaccharide (LPS)-induced septic shock and ALI and showed increased serum IL-1β levels. In addition, BMDMs isolated from BPOZ-2 KO mice showed increased IL-1β production in response to NLRP3 stimuli. Mechanistically, BPOZ-2 interacted with NLRP3 and mediated its degradation by recruiting Cullin 3. In particular, the expression of BPOZ-2 was significantly reduced in lung tissues from mice infected with SARS-CoV-2 and in cells overexpressing SARS-2-N. Importantly, proinflammatory responses triggered by the SARS-2-N were significantly blocked by BPOZ-2 reintroduction. Thus, we concluded that BPOZ-2 is a negative regulator of the NLPR3 inflammasome that likely contributes to SARS-CoV-2-induced hyperinflammation.
Obesity, malnutrition, and the prevalence and outcome of hypertension: Evidence from the National Health and Nutrition Examination Survey
Authors: Zhang Heng-Zhi , Wang Yi-Han , Ge Ying-Lin , Wang Shu-Yu , Sun Jin-Yu , Chen Lu-Lu , Su Shuang , Sun Ying 
Published: March 2023

BackgroundNutritionally unhealthy obesity is a newly introduced phenotype characterized by a combined condition of malnutrition and obesity. This study aims to explore the combined influence of obesity and nutritional status on the prevalence and outcome of hypertension.MethodsParticipants collected from the National Health and Nutrition Examination Survey (NHANES) database were divided into four subgroups according to their obesity and nutritional conditions, as defined by waist circumference and serum albumin concentration. The lean-well-nourished was set as the reference group. Logistic regression models were applied to evaluate the hypertension risk. Kaplan–Meier analysis and Cox proportional hazard regression models were used to assess the survival curve and outcome risk of participants with hypertension.ResultsA total of 28,554 participants with 10,625 hypertension patients were included in the analysis. The lean-malnourished group showed a lower hypertension risk (odds ratio [OR] 0.85, 95% confidence interval [CI]: 0.77–0.94), while the obese-well-nourished condition elevated the risk (OR 1.47, 95% CI: 1.3–1.67). Two malnourished groups had higher mortality risks (HR 1.42, 95% CI: 1.12–1.80 and HR 1.31, 95% CI: 1.03–1.69 for the lean and obese, respectively) than the reference group. The outcome risk of the obese-well-nourished group (HR 1.02, 95% CI: 0.76–1.36) was similar to the lean-well-nourished.ConclusionMalnutrition was associated with a lower risk of developing hypertension in both lean and obese participants, but it was associated with a worse outcome once the hypertension is present. The lean-malnourished hypertension patients had the highest all-cause mortality risk followed by the obese-malnourished. The obese-well-nourished hypertension patients showed a similar mortality risk to the lean-well-nourished hypertension patients.
How supervisors provide and students react to EAL thesis supervision: Voices from Sweden and Indonesia
Authors: Nangimah Musrifatun , Walldén Robert 
Published: March 2023

IntroductionThesis supervision is a critical part of students’ academic literacy development. Previous research has shown different dimensions of this development with limited attention to cross-cultural aspects. In particular, there has been little research on how students and supervisors negotiate supervision practices in non-anglophone contexts. This study aimed to explore students’ and supervisors’ reported priorities and experiences regarding the provision and reception of feedback in English as an Additional Language thesis supervision.MethodWe conducted a qualitative case study to illuminate supervisor’s and students’ experiences of supervision in Sweden and Indonesia. It involved 39 participants (14 supervisors and 25 students) from one Swedish and three Indonesian universities. One-on-one semi-structured interviews were conducted and analyzed thematically using Biesta’s functions of education, Habermas’ communicative action theory, and perspectives on academic literacy.FindingsFirstly, we found that Swedish and Indonesian supervisors had different feedback provision priorities. Swedish supervisors described prioritizing content-focused feedback to facilitate students’ socialization into academic writing. Conversely, most Indonesian supervisors expressed balancing content- and form-focused feedback with a greater emphasis on qualifying as English teachers. Despite these differences, supervisors in both contexts tended to isolate academic language use from discipline-specific values and practices. Secondly, students in both contexts largely expressed an instrumental orientation to achieving their goals and were frustrated by supervisors phrasing feedback as questions. Many students expressed unfamiliarity with necessary methodologies and theoretical frameworks, which made supervisors’ feedback difficult to decode.DiscussionSince only a few of the students viewed the feedback as a support for their process of learning, this study calls for a clear communication about the academic socialization intention through supervision. However, academic socialization cannot solely be the responsibility of supervisors but must be embedded in the curriculum courses.
Associations of serum amino acids related to urea cycle with risk of chronic kidney disease in Chinese with type 2 diabetes
Authors: Zhang Wei , Zheng Jun , Zhang Jikun , Li Ninghua , Yang Xilin , Fang Zhong-Ze , Zhang Qiang 
Published: March 2023

ObjectiveSerum levels of amino acids related to urea cycle are associated with risk of type 2 diabetes mellitus (T2DM). Our study aimed to explore whether serum levels of amino acids related to urea cycle, i.e., arginine, citrulline, and ornithine, are also associated with increased risk of chronic kidney disease (CKD) in T2DM.MethodsWe extracted medical records of 1032 consecutive patients with T2DM from the Electronic Administrative System of Liaoning Medical University First Affiliated Hospital (LMUFAH) system from May 2015 to August 2016. Of them, 855 patients with completed data available were used in the analysis. CKD was defined as estimated glomerular filtration rate (eGFR) &lt;60 mL/min/1.73 m2. Serum amino acids were measured by mass spectrometry (MS) technology. Binary logistic regression was performed to obtain odds ratios (ORs) and their 95% confidence intervals (CIs).Results52.3% of the 855 T2DM patients were male, and 143 had CKD. In univariable analysis, high serum citrulline, high ratio of arginine to ornithine, and low ratio of ornithine to citrulline were associated with markedly increased risk of CKD (OR of top vs. bottom tertile: 2.87, 95%CI, 1.79-4.62 &amp; 1.98, 95%CI,1.25-3.14 &amp; 2.56, 95%CI, 1.61-4.07, respectively). In multivariable analysis, the ORs of citrulline and ornithine/citrulline ratio for CKD remained significant (OR of top vs. bottom tertile: 2.22, 95%CI, 1.29-3.82 &amp; 2.24, 1.29-3.87, respectively).ConclusionsIn Chinese patients with T2DM, high citrulline and low ornithine/citrulline ratio were associated with increased risk of CKD.
Subcutaneous transplantation of human embryonic stem cells-derived pituitary organoids
Authors: Sasaki Hiroo , Suga Hidetaka , Takeuchi Kazuhito , Nagata Yuichi , Harada Hideyuki , Kondo Tatsuma , Ito Eiji , Maeda Sachi , Sakakibara Mayu , Soen Mika , Miwata Tsutomu , Asano Tomoyoshi , Ozaki Hajime , Taga Shiori , Kuwahara Atsushi , Nakano Tokushige , Arima Hiroshi , Saito Ryuta 
Published: March 2023

IntroductionThe pituitary gland, regulating various hormones, is central in the endocrine system. As spontaneous recovery from hypopituitarism is rare, and exogenous-hormone substitution is clumsy, pituitary replacement via regenerative medicine, using pluripotent stem cells, is desirable. We have developed a differentiation method that in mice yields pituitary organoids (POs) derived from human embryonic stem cells (hESC). Efficacy of these POs, transplanted subcutaneously into hypopituitary mice, in reversing hypopituitarism was studied.MethodshESC-derived POs were transplanted into inguinal subcutaneous white adipose tissue (ISWAT) and beneath dorsal skin, a relatively avascular region (AR), of hypophysectomized severe combined immunodeficient (SCID) mice. Pituitary function was evaluated thereafter for ¾ 6mo, assaying basal plasma ACTH and ACTH response to corticotropin-releasing hormone (CRH) stimulation. Histopathologic examination of organoids 150d after transplantation assessed engraftment. Some mice received an inhibitor of vascular endothelial growth factor (VEGF) to permit assessment of how angiogenesis contributed to subcutaneous engraftment.ResultsDuring follow-up, both basal and CRH-stimulated plasma ACTH levels were significantly higher in the ISWAT group (p &lt; 0.001 – 0.05 and 0.001 – 0.005, respectively) than in a sham-operated group. ACTH secretion also was higher in the ISWAT group than in the AR group. Histopathologic study found ACTH-producing human pituitary-cell clusters in both groups of allografts, which had acquired a microvasculature. POs qPCR showed expression of angiogenetic factors. Plasma ACTH levels decreased with VEGF-inhibitor administration.ConclusionsSubcutaneous transplantation of hESC-derived POs into hypopituitary SCID mice efficaciously renders recipients ACTH-sufficient.
Meteorin-like levels are associated with active brown adipose tissue in early infancy
Authors: Garcia-Beltran Cristina , Navarro-Gascon Artur , López-Bermejo Abel , Quesada-López Tania , de Zegher Francis , Ibáñez Lourdes , Villarroya Francesc 
Published: March 2023

IntroductionMeteorin-like (METRNL) is a hormonal factor released by several tissues, including thermogenically active brown and beige adipose tissues. It exerts multiple beneficial effects on metabolic and cardiovascular systems in experimental models. However, the potential role of METRNL as brown adipokine in humans has not been investigated previously, particularly in relation to the metabolic adaptations taking place in early life, when brown adipose tissue (BAT) is particularly abundant.Methods and materialsMETRNL levels, as well as body composition (DXA) and circulating endocrine-metabolic variables, were assessed longitudinally in a cohort of infants at birth, and at ages 4 and 12 months. BAT activity was measured by infrared thermography at age 12 months. METRNL levels were also determined cross-sectionally in adults; METRNL gene expression (qRT-PCR) was assessed in BAT and liver samples from neonates, and in adipose tissue and liver samples form adults. Simpson-Golabi-Behmel Syndrome (SGBS) adipose cells were thermogenically activated using cAMP, and METRNL gene expression and METRNL protein released were analysed.ResultsSerum METRNL levels were high at birth and declined across the first year of life albeit remaining higher than in adulthood. At age 4 and 12 months, METRNL levels correlated positively with circulating C-X-C motif chemokine ligand 14 (CXCL14), a chemokine released by thermogenically active BAT, but not with parameters of adiposity or metabolic status. METRNL levels also correlated positively with infrared thermography-estimated posterior-cervical BAT activity in girls aged 12 months. Gene expression analysis indicated high levels of METRNL mRNA in neonatal BAT. Thermogenic stimulus of brown/beige adipocytes led to a significant increase of METRNL gene expression and METRN protein release to the cell culture medium.ConclusionCirculating METRNL levels are high in the first year of life and correlate with indices of BAT activity and with levels of an established brown adipokine such as CXCL14. These data, in addition with the high expression of METRNL in neonatal BAT and in thermogenically-stimulated brown/beige adipocytes, suggest that METRNL is actively secreted by BAT and may be a circulating biomarker of BAT activity in early life.
Quantitative assessment of the degree of harmony between humanity and nature for national parks in China: A case study of the Three-River-Source National Park
Authors: Guo Yanming , Liu Xiaojie , Liu Xiaohuang , Zhang Jiahong , Zhang Haiyan , Fan Jiangwen , Khan Nawab , Ma Jiliang 
Published: March 2023

IntroductionNational parks, defined as the mainstay of the nature reserve system in China, pursue to achieve scientific protection and rational utilization of natural resources. However, eco-environmental and socioeconomic benefits are rarely considered together. Hereby, how to quantitatively express the relationship between humanity and nature in national parks needs further exploration. We selected the Three-River-Source National Park (TRSNP), China's largest national park by area and the world's highest altitude national park, as a representative case to construct an evaluation model for the degree of harmony between humanity and the nature of the national alpine ecological park.MethodsBased on the field survey data, the meteorological data, the remote sensing data, and the socioeconomic data, the study used the model inversion and the spatial analysis methods to quantitatively evaluate the degree of harmony consisting of 12 indexes from a fresh perspective of a combination of the ecological environment and social economy. Considering the TRSNP establishment in 2016 as the time node, we assessed and compared the degree of harmony between humanity and nature during the dynamic baseline period (2011–2015) and the evaluation period (2016–2020).ResultsThe results show that the degree of harmony between humanity and nature showed a gradual upward trend from the northwest to the southeast in the TRSNP. Compared with the dynamic baseline period, the eco-environmental and socioeconomic levels of the evaluation period were increased by 34.48 and 5.46%, respectively. Overall, the degree of harmony between humanity and nature visibly increased by 23.38%.DiscussionThis study has developed a novel comprehensive method for evaluating national parks at the regional scale for the win-win goal of both protection and development, and it provides a theoretical basis for effective planning and management policies for national parks.
Climate change and maple syrup: Producer observations, perceptions, knowledge, and adaptation strategies
Authors: Ahmed Selena , Lutz David , Rapp Joshua , Huish Ryan , Dufour Boris , Brunelle Autumn , Morelli Toni Lyn , Stinson Kristina , Warne Teresa 
Published: March 2023

IntroductionClimate change is impacting forest-based agricultural systems with implications for producer decision-making and livelihoods. This article presents a case study on the observations, perceptions, knowledge, and adaptation strategies of maple syrup producers in the United States to climate change.MethodsWe carried out two semi-structured surveys with maple producers on: (1) climate change and its impacts on the maple system (n = 106 participants); and (2) responses to climate adaptation scenarios (n = 98 participants). Additionally, we carried out two focus groups and key informant interviews (n = 70+) to understand barriers and opportunities for climate adaptation. One of these focus groups and follow up key informant interviews was with tribally affiliated community members with the intention to acknowledge Indigenous Peoples’ voices, history, and relationships to the land.ResultsFindings highlight that most of the surveyed producers (89%) have experienced the negative impacts of climate on maple syrup production. While 40% of participants feel concerned regarding the future of the maple system, 39% feel hopeful, with significant differences based on the age of the surveyed producers. The majority of producers have adapted their harvesting practices to climate effects. Producers shared knowledge of multiple adaptation strategies in response to climate scenarios comprised of: (1) stand management practices such as diversification of sap species tapped; (2) harvesting practices such as changing the type and number of taps; (3) sap processing practices focused on the integration of technology such as the use of an evaporator and reverse osmosis; and (4) marketing practices such as innovation of products and marketing different maple syrup characteristics. Responses shared by tribally affiliated producers highlight knowledge of multiple adaptation strategies that focus on long-term ecological management of forests rather than technological solutions.DiscussionOverall, findings emphasize the importance of cooperation and diversification at every level and dimension of the maple system for its long-term resilience.