bluuebunny/crossref_metadata_2025_split huggingface dataset:
Unravelling human hematopoietic progenitor cell diversity through association with intrinsic regulatory factors
Authors: Favaro Patricia , Glass David R. , Borges Luciene , Baskar Reema , Reynolds Warren , Ho Daniel , Bruce Trevor , Tebaykin Dmitry , Scanlon Vanessa M. , Shestopalov Ilya , Bendall Sean C. 
Published: August 2023

SummaryHematopoietic stem and progenitor cell (HSPC) transplantation is an essential therapy for hematological conditions, but finer definitions of human HSPC subsets with associated function could enable better tuning of grafts and more routine, lower-risk application. To deeply phenotype HSPCs, following a screen of 328 antigens, we quantified 41 surface proteins and functional regulators on millions of CD34+ and CD34- cells, spanning four primary human hematopoietic tissues: bone marrow, mobilized peripheral blood, cord blood, and fetal liver. We propose more granular definitions of HSPC subsets and provide new, detailed differentiation trajectories of erythroid and myeloid lineages. These aspects of our revised human hematopoietic model were validated with corresponding epigenetic analysis andin vitroclonal differentiation assays. Overall, we demonstrate the utility of using molecular regulators as surrogates for cellular identity and functional potential, providing a framework for description, prospective isolation, and cross-tissue comparison of HSPCs in humans.
HCR spectral imaging: 10-plex, quantitative, high-resolution RNA and protein imaging in highly autofluorescent samples
Authors: Schulte Samuel J. , Fornace Mark E. , Hall John K. , Pierce Niles A. 
Published: August 2023

ABSTRACTSignal amplification based on the mechanism of hybridization chain reaction (HCR) provides a unified framework for multiplex, quantitative, high-resolution imaging of RNA and protein targets in highly autofluorescent samples. With conventional bandpass imaging, multiplexing is typically limited to four or five targets due to the difficulty in separating signals generated by fluorophores with overlapping spectra. Spectral imaging has offered the conceptual promise of higher levels of multiplexing, but it has been challenging to realize this potential in highly autofluorescent samples including whole-mount vertebrate embryos. Here, we demonstrate robust HCR spectral imaging with linear unmixing, enabling simultaneous imaging of 10 RNA and/or protein targets in whole-mount zebrafish embryos and mouse brain sections. Further, we demonstrate that the amplified and unmixed signal in each of 10 channels is quantitative, enabling accurate and precise relative quantitation of RNA and/or protein targets with subcellular resolution, and RNA absolute quantitation with single-molecule resolution, in the anatomical context of highly autofluorescent samples.SUMMARYSpectral imaging with signal amplification based on the mechanism of hybridization chain reaction enables robust 10-plex, quantitative, high-resolution imaging of RNA and protein targets in whole-mount vertebrate embryos and brain sections.
Respiratory syncytial virus-associated hospitalisation in adults with comorbidities in two European countries
Authors: Osei-Yeboah Richard , Johannesen Caroline , Egeskov-Cavling Amanda Marie , Chen Junru , Lehtonen Toni , Fornes Arantxa Urchueguía , Paget John , Fischer Thea K. , Wang Xin , Nair Harish , Campbell Harry 
Published: August 2023

AbstractBackgroundIndividuals with comorbidities are at increased risk of severe RSV infection. We estimated RSV-associated respiratory tract infection (RTI) hospitalisation among adults aged 45 years and older with comorbidities in Denmark and Scotland.MethodsBy analysing national hospital and virological data, we estimated annual average number and rate of RSV-associated hospitalisations by seven selected comorbidities and age during 2010-2018. We estimated rate ratios (RRs) of RSV-associated hospitalisation and 95% uncertainty ranges in comorbid adults versus the overall populations.ResultsIn Danish adults aged 45y+, RSV-RTI hospitalisation rates ranged from 3.1 per 1000 individuals with asthma, to 19.4 per 1000 individuals per year with chronic kidney disease (CKD). In Scotland, the rate ranged from 2.4 per 1000 individuals per year with chronic liver disease (CLD), to 9.0 per 1000 individuals per year with chronic obstructive pulmonary disease (COPD). In both countries, we found over 2-4-fold increased risk of RSV hospitalisation in adults with COPD, ischemic heart disease (IHD), stroke and diabetes, and 1.5-3-fold increased risk for adults with asthma and 3-7-fold for those with CKD. RSV hospitalisation rates among adults aged 45-64y with COPD, asthma, IHD or CKD were higher compared with the overall population aged 65-74y.ConclusionFindings of this study provide important evidence for identifying risk groups and assisting health authorities in RSV vaccination policy making.
Foreign language effect in negotiations: negotiation language and framing effect on contract terms and subjective outcomes
Authors: Lee Jung Hyun , Elfenbein Hillary Anger , Bottom William P. 
Published: August 2023

Purpose
This study aims to test negotiation outcomes when bilinguals negotiate in a foreign rather than their native language. Decision research on the foreign language effect indicates that bilingual individuals may be less susceptible to framing bias when using a foreign language because they make less emotional and biased choices. With increasing international business activity, there is a pressing need to examine the effect of language on bilingual negotiators.

Design/methodology/approach
The authors tested the hypotheses using a two (task frame: gain vs loss) × 2 (language: foreign vs native) factorial design recruiting 246 Korean–English bilinguals. A negotiation simulation with three issues was used, and participants exchanged offers with a preprogrammed computer they believed to be a real counterpart.

Findings
There was no significant interaction effect between framing and language on the offers made, but the framing effect was mitigated and nonsignificant for negotiators who used their foreign language. The interaction between framing and language conditions significantly affected negotiators’ positive emotions and satisfaction with the negotiation.

Originality/value
The uniqueness of this paper is related to its effort to investigate the effect of negotiation language on a negotiator’s decision-making. Considering globalization and the increasing prevalence of international negotiations, this paper has implications for researchers and practitioners.
Impact of entrepreneurial education, trait competitiveness and psychological capital on entrepreneurial behavior of university students in GCC
Authors: Abubakar Ahmed , Mourgan Fatma Hassan Abdelbasset , Al Kharusi Buthaina , Elfitori Chadilia Mohammed 
Published: August 2023

Purpose
This research is in response to the growing need for economic diversification in the Gulf Cooperation Council (GCC) countries as they seek to manage the impacts of volatile oil revenues. To this end, governments in the region are encouraging their citizens to undertake entrepreneurial activities. The purpose of this study is to examine the effects of entrepreneurial education (EE), trait competitiveness (TC) and psychological capital (PC) on the entrepreneurial behavior (EB) of university students.

Design/methodology/approach
As a quantitative study, primary data were collected through a survey administered to the team of young university students in Oman. Using stratified sampling techniques, 858 questionnaires were retrieved and used for the analysis out of the 1,176 sample. The analysis of the data was done using structural equation modeling.

Findings
The results have established a positive influence of EE, TC and PC on the EB of university students in the GCC region. This study also found that PC acts as a bridge between EE, TC and EB.

Originality/value
This paper provides further literature evidence on the contribution of other variables like TC and PC to EB, particularly in the GCC context, by providing a model of the influence mechanism on EB. This research reveals a very unique influence of PC on the relationship between TC and EB. The findings confirm that there is no direct relationship between TC and EB. However, the relationship was established with the introduction of PC.
Data‐driven identification of major axes of functional variation in bacteria
Authors: Lajoie Geneviève , Kembel Steven W. 
Published: August 2023

AbstractThe discovery of major axes of correlated functional variation among species and habitats has revealed the fundamental trade‐offs structuring both functional and taxonomic diversity in eukaryotes such as plants. Whether such functional axes exist in the bacterial realm and whether they could explain bacterial taxonomic turnover among ecosystems remains unknown. Here, we use a data‐driven approach to leverage global genomic and metagenomic datasets to reveal the existence of major axes of functional variation explaining both evolutionary differentiation within Bacteria and their ecological sorting across diverse habitats. We show that metagenomic variation among bacterial communities from various ecosystems is structured along a few axes of correlated functional pathways. Similar clusters of traits explained phylogenetic trait variation among >16,000 bacterial genomes, suggesting that functional turnover among bacterial communities from distinct habitats does not only result from the differential filtering of similar functions among communities, but also from phylogenetic correlations among these functions. Concordantly, functional pathways associated with trait clusters that were most important for defining functional turnover among bacterial communities were also those that had the highest phylogenetic signal in the bacterial genomic phylogeny. This study overall underlines the important role of evolutionary history in shaping contemporary distributions of bacteria across ecosystems.
Mutations of voltage‐gated sodium channel contribute to pyrethroid resistance in Panonychus citri
Authors: Pan Deng , Luo Qiu‐Juan , O´Reilly Andrias O. , Yuan Guo‐Rui , Wang Jin‐Jun , Dou Wei 
Published: August 2023

AbstractInsecticide resistance in Panonychus citri is a major obstacle to mite control in citrus orchards. Pyrethroid insecticides are continually used to control mites in China, although resistance to pyrethroids has evolved in some populations. Here, the resistance to the pyrethroid fenpropathrin was investigated and 7 out of 8 field‐collected populations of P. citri exhibited a high level of resistance, ranging from 171‐fold to 15 391‐fold higher than the susceptible (SS) comparison strain. Three voltage‐gated sodium channel (VGSC) mutations were identified in the tested populations: L1031V, F1747L, and F1751I. Amplicon sequencing was used to evaluate the frequency of these mutations in the 19 field populations. L1031V and F1751I were present in all populations at frequencies of 11.6%–82.1% and 0.5%–31.8%, respectively, whereas the F1747L mutation was only present in 12 populations from Chongqing, Sichuan, Guangxi, and Yunnan provinces. Introduction of these mutations singly or in combination into transgenic flies significantly increased their resistance to fenpropathrin and these flies also exhibited reduced mortality after exposure to the pyrethroids permethrin and β‐cypermethrin. Panonychus citri VGSC homology modeling and ligand docking indicate that F1747 and F1751 form direct binding contacts with pyrethroids, which are lost with mutation, whereas L1031 mutation may diminish pyrethroid effects through an allosteric mechanism. Overall, the results provide molecular markers for monitoring pest resistance to pyrethroids and offer new insights into the basis of pyrethroid actions on sodium channels.
The consequences of neglecting reservoir storage in national‐scale hydrologic models: An appraisal of key streamflow statistics
Authors: Hodgkins Glenn A. , Over Thomas M. , Dudley Robert W. , Russell Amy M. , LaFontaine Jacob H. 
Published: August 2023

AbstractA better understanding of modeled streamflow errors related to basin reservoir storage is needed for large regions, which normally have many ungaged basins with reservoirs. We quantified the difference between modeled and observed streamflows for one process‐based and three statistical‐transfer hydrologic models, none of which explicitly accounted for reservoir storage. Streamflow statistics representing low to high flows, seasonality, annual variability, and daily autocorrelation were examined at 1082 study basins across the conterminous USA. All models increasingly overpredict (or decreasingly underpredict) observed annual maximum flows with increasing storage. Correlations between absolute values of errors for low‐flow statistics and storage are often larger in magnitude than those for signed errors—additional storage is associated with increases in model errors in both directions even when its overall effect in one direction is weak. The rate of increase in absolute values of model errors was nonlinear for most statistics. For low flows, model errors had a change point to larger errors at 48 days of reservoir storage (relative to long‐term mean daily flow); mean and high flows had change points at 147 to 176 days. We present predicted‐to‐observed errors for nine streamflow statistics over a large range of reservoir storage to help modelers and users of modeled streamflow understand the amount of storage for which explicit reservoir modeling is needed.
Endogenous opioid system modulates conditioned cocaine reward in a sex‐dependent manner
Authors: Matsumura Kanako , Nicot Amélia , Choi In Bae , Asokan Meera , Le Nathan N. , Natividad Luis A. , Dobbs Lauren K. 
Published: August 2023

AbstractCocaine predictive cues and contexts exert powerful control over behaviour and can incite cocaine seeking and taking. This type of conditioned behaviour is encoded within striatal circuits, and these circuits and behaviours are, in part, regulated by opioid peptides and receptors expressed in striatal medium spiny neurons. We previously showed that augmenting levels of the opioid peptide enkephalin in the striatum facilitates acquisition of cocaine conditioned place preference (CPP), while opioid receptor antagonists attenuate expression of cocaine CPP. However, whether striatal enkephalin is necessary for acquisition of cocaine CPP and maintenance during extinction remains unknown. To address this, we generated mice with a targeted deletion of enkephalin from dopamine D2‐receptor expressing medium spiny neurons and tested them in a cocaine CPP paradigm. Low striatal enkephalin levels did not attenuate acquisition of CPP. However, expression of preference, assessed after acute administration of the opioid receptor antagonist naloxone, was blocked in females, regardless of genotype. When saline was paired with the cocaine context during extinction sessions, females, regardless of genotype, extinguished preference faster than males, and this was prevented by naloxone when paired with the cocaine context. We conclude that while striatal enkephalin is not necessary for acquisition, expression, or extinction of cocaine CPP, expression and extinction of cocaine preference in females is mediated by an opioid peptide other than striatal enkephalin. The unique sensitivity of females to opioid antagonists suggests sex should be a consideration when using these compounds in the treatment of cocaine use disorder.
Psoriasiform graft‐versus‐host disease responded to secukinumab: A case report
Authors: Gu Ying , Sun Jing , Zhang Jianzhong 
Published: August 2023

AbstractWe describe a 9‐year‐old boy who developed generalised erythematous lesions 12 months after haematopoietic stem cell transplant (HSCT). Histopathology showed both features of psoriasis and graft‐versus‐host disease (GVHD). The lesions responded well to secukinumab, suggesting that IL‐17A monoclonal antibody might be a treatment option for GVHD.
Insights into the daily life of ventricular assist device coordinators: Outcome of an international questionnaire
Authors: Mulzer Johanna , Kaufmann Friedrich , Mueller Marcus , Potapov Evgenij , Knierim Jan 
Published: August 2023

AbstractBackgroundAs the incidence of heart failure in developed countries is on the rise, mechanical circulatory support (MCS) often remains the only treatment option for patients with end‐stage heart failure and is well established worldwide. Even though VAD coordinators play a key role in VAD programs, their responsibilities and daily duties are not clearly defined and characterized. Recently published data from the first 5‐year multicenter clinical trial assessing experience with the HeartMate 3 left ventricular system (Abbott, Abbott Park, IL) show an overall survival of 61% at 5 years. When it comes to possible improvements to these systems, it is necessary for developers not only to know the status quo but also to determine and consider the visions and wishes of those individuals who take care of patients, provide education and deal with possible complications. This would be helpful a meaningful effort towards implementing a standard of care.MethodsTo fill this knowledge gap, we conducted an online survey using the SurveyMonkey tool, addressing representatives of programs implanting VADs worldwide. Representatives answered a standardized block of 14 questions and were asked to provide responses within 3 months.ResultsA total of 91 VAD coordinators from centers of various regions of the world completed the survey. The majority came from European countries. The numbers of patients followed up by the centers ranged from <20 to 390 patients. The majority of VAD coordinators had a nursing background (68%). Seventy‐seven percent of the centers operate a 24‐h hotline and 3‐monthly visits to the outpatient department are most common. Fifty‐nine percent of the centers do not use an infection scoring system for driveline wound care. The majority of the centers indicated that an optimized follow‐up concept including wound care, medication, and social care is crucial and the key issue for an improved outcome. Smaller components and intensified psychosocial support ranked highest in questions about how to improve quality of life. Surprisingly, the future prospects of telemetric monitoring were not rated high in significance.ConclusionsThere is a wide variability in the composition and tasks of VAD programs worldwide. Implementing a standard of care and improving psychosocial care as well as equipment is regarded most important to improve outcomes and quality of life. From the point of view of those responsible, the significance of telemetric monitoring seemed overrated.
Development of novel tracers for sentinel node identification in cervical cancer
Authors: Kodama Keisuke , Tateishi Chuya , Oda Tsuyoshi , Cui Lin , Kuramoto Kazutaka , Yahata Hideaki , Okugawa Kaoru , Maenohara Shoji , Yagi Hiroshi , Yasunaga Masafumi , Onoyama Ichiro , Asanoma Kazuo , Mori Takeshi , Katayama Yoshiki , Kato Kiyoko 
Published: August 2023

AbstractIndocyanine green (ICG) with near‐infrared (NIR) fluorescence imaging is used for lymphatic mapping. However, binding of ICG to blood proteins like serum albumin can shorten its retention time in sentinel lymph nodes (SLNs). Here, we investigated the efficacy and safety of a new fluorescence tracer comprising phytate and liposome (LP)‐encapsulated ICG. Coadministration of phytate with LP containing phosphatidic acid promotes chelation mediated by Ca2+ in bodily fluids to enhance SLN retention. Uniformly sized LPs (100 nm) encapsulating ICG under conditions that minimized fluorescence self‐quenching during storage were produced. We analyzed the behavior of the new tracer (ICG‐phytate‐LP) and control tracers (ICG and ICG‐LP) in the lymphatic flow of mice in terms of lymph node retention time. We also tested lymphatic flow and safety in pigs that have a more human‐like lymphatic system. LPs encapsulating stabilized ICG were successfully prepared. Mixing LP with phytate in the presence of Ca2+ increased both the particle size and negative surface charge. In mice, ICG‐phytate‐LP had the best lymph node retention, with a fluorescence intensity ratio that increased over 6 h and then decreased slowly over the next 24 h. In pigs, administration of ICG and ICG‐phytate‐LP resulted in no death or weight loss. There were no obvious differences between blood test results for the ICG and ICG‐phytate‐LP groups, and the overall safety was good. ICG‐phytate‐LP may be a useful new tracer for gynecological cancers that require time for lymph node identification due to a retroperitoneal approach.
Schizophrenia mediating the effect of smoking phenotypes on antisocial behavior: A Mendelian randomization analysis
Authors: Zhang Minghui , Tang Jie , Li Wei , Xue Kaizhong , Wang Zirui , Chen Yayuan , Xu Qiang , Zhu Dan , Cai Mengjing , Ma Juanwei , Yao Jia , Zhang Yijing , Wang He , Liu Feng , Guo Lining 
Published: August 2023

AbstractAimsPrevious studies have indicated that smoking is linked to an increased risk of developing schizophrenia, and that individuals with schizophrenia are more prone to engaging in antisocial behavior. However, the causal effects of smoking behaviors on antisocial behavior and the potential mediating role of schizophrenia remains largely unclear.MethodsIn the present study, using the summary data from genome wide association studies of smoking phenotypes (N = 323,386–805,431), schizophrenia (Ncases = 53,386, Ncontrols = 77,258), and antisocial behavior (N = 85,359), we assessed bidirectional causality between smoking phenotypes and schizophrenia by the Mendelian randomization (MR) approach. Using a two‐step MR approach, we further examined whether causal effects of smoking phenotypes/schizophrenia on antisocial behavior were mediated by schizophrenia/smoking phenotypes.ResultsThe results showed that smoking initiation (SmkInit) and age of smoking initiation (AgeSmk) causally increase the risk of schizophrenia (SmkInit: OR = 2.06, 95% CI = 1.77–2.39, p = 4.36 × 10−21; AgeSmk: OR = 0.32, 95% CI = 0.16–0.62, p = 8.11 × 10−4, Bonferroni corrected). However, there was no causal effect that liability to schizophrenia leads to smoking phenotypes. MR evidence also revealed causal influences of SmkInit and the amount smoked (CigDay) on antisocial behavior (SmkInit: OR = 1.28, 95% CI = 1.17–1.41, p = 2.53 × 10−7; CigDay: OR = 1.16, 95% CI = 1.06–1.27, p = 1.60 × 10−3, Bonferroni corrected). Furthermore, the mediation analysis indicated that the relationship between SmkInit and antisocial behavior was partly mediated by schizophrenia (mediated proportion = 6.92%, 95% CI = 0.004–0.03, p = 9.66 × 10−3).ConclusionsThese results provide compelling evidence for taking smoking interventions as a prevention strategy for schizophrenia and its related antisocial behavior.