bluuebunny/crossref_metadata_2025_split huggingface dataset:
Parry–Romberg syndrome—Still progressing over 20 years of disease course
Authors: Thoibisana Ashem , Mishra Biswamohan , Elavarasi Arunmozhimaran , Singh Rajesh Kumar , Vibha Deepti , Gaikwad Shailesh , Tripathi Manjari 
Published: June 2023

AbstractParry–Romberg syndrome presents with atrophy of one‐half of the face involving skin, soft tissue, muscle, and bone. It usually progresses slowly over 2–20 years and finally stabilizes. Neuropsychiatric abnormalities are subtle, seen in about 10% of the patients, and include cognitive disturbances, aphasia, hallucination, and other psychiatric disorders. We describe the case of a young female who had progressive brain atrophy over 20 years of disease course and presented with focal seizures and recent onset progressive cognitive impairment.
The proteinuria‐lowering effects of dapagliflozin are associated with an initial decline in estimated glomerular filtration rate in patients with chronic kidney disease
Authors: Murakoshi Maki , Kobayashi Takashi , Kihara Masao , Ueda Seiji , Suzuki Yusuke , Gohda Tomohito 
Published: June 2023

AbstractAimSodium‐glucose co‐transporter‐2 inhibitor, dapagliflozin (DAPA) reduced albuminuria and slowed down the decline in estimated glomerular filtration rate (eGFR) in patients with chronic kidney disease (CKD) in the DAPA‐CKD trial. However, proteinuria (albuminuria) does not necessarily decrease in all patients in real‐world clinical settings. Therefore, we aimed to identify the clinical characteristics of patients with CKD and decreased proteinuria in response to DAPA treatment.MethodsOf 106 patients with CKD, 54 patients were finally included who received 10 mg of DAPA once daily. Patients whose urinary protein‐to‐creatinine ratio (UPCR) decreased by >30% or ≤30% from baseline after 1 month of treatment were defined as responders and non‐responders, respectively.ResultsAt baseline, median eGFR and UPCR were 45.3 mL/min/1.73 m2 (interquartile range [IQR], 29.7, 54.6) and 1.09 g/gCr (IQR, 0.52, 1.91), respectively. After 1 month of treatment, the mean decline in eGFR and reduction in UPCR was 6.5% (standard deviation [SD], 7.2%) and 6.6% (SD, 42.1%) from baseline, respectively. Moreover, the blood pressure, eGFR, and uric acid decreased significantly from baseline, but haemoglobin and serum potassium did not change. The median UPCR decreased significantly in patients with UPCR ≥0.5 g/gCr, but not <0.5 g/gCr at baseline. UPCR responders had a greater initial decline in eGFR at 1 month than non‐responders.ConclusionThe percent changes in UPCR were positively associated with the initial decline rate in eGFR in patients with CKD with a UPCR ≥0.5 g/gCr at baseline after 1 month of DAPA treatment.
Tryptophan‐synthesizing bacteria enhance colonic motility
Authors: Legan Theresa B. , Lavoie Brigitte , Norberg Emilia , Ley Isabella C. , Tack Stephanie , Tompkins Thomas A. , Wargo Matthew J. , Mawe Gary M. 
Published: June 2023

AbstractBackgroundAn emerging strategy to treat symptoms of gastrointestinal (GI) dysmotility utilizes the administration of isolated bacteria. However, the underlying mechanisms of action of these bacterial agents are not well established. Here, we elucidate a novel approach to promote intestinal motility by exploiting the biochemical capability of specific bacteria to produce the serotonin (5‐HT) precursor, tryptophan (Trp).MethodsMice were treated daily for 1 week by oral gavage of Bacillus (B.) subtilis (R0179), heat‐inactivated R0179, or a tryptophan synthase‐null strain of B. subtilis (1A2). Tissue levels of Trp, 5‐HT, and 5‐hydroxyindoleacetic acid (5‐HIAA) were measured and changes in motility were evaluated.Key resultsMice treated with B. subtilis R0179 exhibited greater colonic tissue levels of Trp and the 5‐HT breakdown product, 5‐HIAA, compared to vehicle‐treated mice. Furthermore, B. subtilis treatment accelerated colonic motility in both healthy mice as well as in a mouse model of constipation. These effects were not observed with heat‐inactivated R0179 or the live 1A2 strain that does not express tryptophan synthase. Lastly, we found that the prokinetic effects of B. subtilis R0179 were blocked by coadministration of a 5‐HT4 receptor (5‐HT4R) antagonist and were absent in 5‐HT4R knockout mice.Conclusions and inferencesTaken together, these data demonstrate that intestinal motility can be augmented by treatment with bacteria that synthesize Trp, possibly through increased 5‐HT signaling and/or actions of Trp metabolites, and involvement of the 5‐HT4R. Our findings provide mechanistic insight into a transient and predictable bacterial strategy to promote GI motility.
Listening to plant's Esperanto via root exudates: reprogramming the functional expression of plant growth‐promoting rhizobacteria
Authors: Feng Haichao , Fu Ruixin , Luo Jiayu , Hou Xueqin , Gao Kun , Su Lv , Xu Yu , Miao Youzhi , Liu Yunpeng , Xu Zhihui , Zhang Nan , Shen Qirong , Xun Weibing , Zhang Ruifu 
Published: June 2023

Summary
Rhizomicrobiome plays important roles in plant growth and health, contributing to the sustainable development of agriculture. Plants recruit and assemble the rhizomicrobiome to satisfy their functional requirements, which is widely recognized as the ‘cry for help’ theory, but the intrinsic mechanisms are still limited.
In this study, we revealed a novel mechanism by which plants reprogram the functional expression of inhabited rhizobacteria, in addition to the de novo recruitment of soil microbes, to satisfy different functional requirements as plants grow. This might be an efficient and low‐cost strategy and a substantial extension to the rhizomicrobiome recruitment theory.
We found that the plant regulated the sequential expression of genes related to biocontrol and plant growth promotion in two well‐studied rhizobacteria Bacillus velezensis SQR9 and Pseudomonas protegens CHA0 through root exudate succession across the plant developmental stages. Sixteen key chemicals in root exudates were identified to significantly regulate the rhizobacterial functional gene expression by high‐throughput qPCR.
This study not only deepens our understanding of the interaction between the plant–rhizosphere microbiome, but also provides a novel strategy to regulate and balance the different functional expression of the rhizomicrobiome to improve plant health and growth.
Is chloroplast size optimal for photosynthetic efficiency?
Authors: Głowacka Katarzyna , Kromdijk Johannes , Salesse‐Smith Coralie E. , Smith Cailin , Driever Steven M. , Long Stephen P. 
Published: June 2023

Summary
Improving photosynthetic efficiency has recently emerged as a promising way to increase crop production in a sustainable manner. While chloroplast size may affect photosynthetic efficiency in several ways, we aimed to explore whether chloroplast size manipulation can be a viable approach to improving photosynthetic performance.
Several tobacco (Nicotiana tabacum) lines with contrasting chloroplast sizes were generated via manipulation of chloroplast division genes to assess photosynthetic performance under steady‐state and fluctuating light. A selection of lines was included in a field trial to explore productivity.
Lines with enlarged chloroplasts underperformed in most of the measured traits. Lines with smaller and more numerous chloroplasts showed a similar efficiency compared with wild‐type (WT) tobacco. Chloroplast size only weakly affected light absorptance and light profiles within the leaf. Increasing chloroplast size decreased mesophyll conductance (gm) but decreased chloroplast size did not increase gm. Increasing chloroplast size reduced chloroplast movements and enhanced non‐photochemical quenching. The chloroplast smaller than WT appeared to be no better than WT for photosynthetic efficiency and productivity under field conditions.
The results indicate that chloroplast size manipulations are therefore unlikely to lead to higher photosynthetic efficiency or growth.
What has philosophy ever done for nursing: A discursive shift from margins to mainstream
Authors: Georges Jane M. 
Published: June 2023

AbstractThis paper is a personal dialogue of maneuvering the landscape of scholarship in the United States as a nurse faculty. The principal thesis of this paper is that a discursive shift from margins to mainstream literature has occurred within nursing discourse during the past 20 years as the result of a growing body of work by nurse philosophers. I utilize my own work in nursing philosophy as an exemplar and provide a narrative situated in a feminist‐critical paradigm. This paper: (1) presents a historical background through a critical‐feminist lens of the discursive shift using my own work and lived experiences as exemplars; (2) examines a contemporary mainstream ‘authoritative’ text as an exemplar of this discursive shift and (3) proposes both potential positive intersections and threats in the future development of nursing philosophy resulting from this discursive shift.
Impacts of nutrition counseling on depression and obesity: A scoping review
Authors: Milligan Meredith N. , Duemling Kathleen , Radovanovic Natasa , Alkozah Maria , Riblet Natalie 
Published: June 2023

SummaryThis scoping review aims to evaluate the impact of nutrition counseling on mental health and wellbeing among people affected by obesity. Depression and obesity are major sources of morbidity and mortality worldwide. The prevalence of obesity is higher in patients with severe or suboptimally managed depression. Change in dietary quality prompted by nutrition counseling may pose a unique opportunity for intervention. Of the 1745 studies identified, 26 studies (total n = 6727) met inclusion criteria. Due to the heterogeneity of methods and outcome reporting, it was not possible to perform meta‐analysis. Across all included studies, 34 different scales were used to quantify mental health/wellbeing. Eleven studies (42.3%) reported statistically significant findings between intervention and control groups. Only two of these studies assessed nutrition counseling independently rather than as part of a multidisciplinary intervention. Overall, many studies have examined the role of nutrition counseling on mental health/wellbeing in individuals affected by obesity. However, due to inconsistency in study methodologies and outcome measurement tools, it is challenging to draw robust or clinically meaningful conclusions about the effects of nutrition counseling on mental health in this population.
Dental caries in HIV‐infected children and adolescents: A systematic review with meta‐analysis
Authors: Faé Daniele Sorgatto , de Aquino Sibele Nascimento , Verner Francielle Silvestre , Lemos Cleidiel Aparecido Araujo 
Published: June 2023

AbstractThis study aimed to evaluate and compare the risk of dental caries in between human immunodeficiency virus (HIV)‐infected and uninfected children and adolescents. An electronic search was performed on PubMed/MEDLINE, Web‐of‐Science, Scopus, LILACS, ProQuest, and ClinicalTrials.gov up to May 2022. The critical appraisal checklist developed by the Joanna Briggs Institute was used to evaluate the quality of the included studies. Meta‐analysis was performed using the RevMan 5.4. Sixteen studies were included. A total of 3231 participants were evaluated, including 1701 and 1530 HIV‐infected and uninfected children and adolescents, respectively. The meta‐analysis revealed a higher risk of dental caries for primary dentitions in HIV‐infected children and adolescents by decayed, missing, filled tooth (dmft) (SMD:0.34; p = 0.006) and decayed, missing, filled surface (dmfs) scores (SMD:0.37; p = 0.001). Similar results were observed for permanent dentition, with increased dental caries in HIV‐infected children and adolescents with DMFT (SMD:0.32; p = 0.003) and DMFS (SMD:1.78; p < 0.0001) scores. Regarding the quality assessment, most of the included studies were moderate or good quality. However, the certainty of the evidence of the outcomes was very low. This systematic review and meta‐analysis showed higher caries severity in permanent and deciduous teeth among HIV‐infected children and adolescents.
Palmitic acid induces GSDMD‐mediated pyroptosis in periodontal ligament cells via the NF‐κB pathway
Authors: Sun Bing‐Jing , Chen Mei‐Hua , Zhang Wei‐Hua , Zhao Bing‐Jiao , Zhang Meng‐Han , Han Xin‐Xin , Yu Li‐Ming , Liu Yue‐Hua 
Published: June 2023

AbstractObjectiveObesity can affect periodontal tissues and exacerbate periodontitis. Pyroptosis, a newly identified type of inflammatory cell death, is involved in the development of periodontal inflammation. The saturated fatty acid palmitic acid (PA) is elevated in obese patients. The effect of PA on pyroptosis in periodontal ligament cells (PDLCs) and its underlying mechanisms remain unknown.Materials and MethodsHuman PDLCs were isolated from healthy individuals and cultured for experiments. The effects of PA on PDLC pyroptosis and the underlying mechanisms were examined by transmission electron microscopy, quantitative real‐time PCR and western blotting.ResultsThe morphology of PDLCs in the PA group indicated pyroptotic characteristics, including swollen cells, plasma membrane rupture and changes in subcellular organelles. PA induced inflammatory responses in PDLCs, as indicated by an increase in IL‐1β in the cell culture supernatant. Furthermore, we found that the pyroptosis‐related proteins caspase‐1, caspase‐4 and GSDMD were involved in PA‐induced cell death. GSDMD and caspase‐4 inhibitors alleviated pyroptotic death of PDLCs. Moreover, PA promoted NF‐κB P65 phosphorylation. A NF‐κB inhibitor decreased IL‐1β expression and partly rescued cell death induced by PA.ConclusionPA activated the NF‐κB pathway and induced the inflammatory response in PDLCs. Caspase‐4/GSDMD mediated PDLC pyroptosis induced by PA.
miR‐148a‐3p regulates proliferation and apoptosis of idiopathic gingival fibroma by targeting NPTX1
Authors: Sun Yuyang , Xie Liangkun , Ren Xiaobin , Ran Liquan , He Hongbing , Kong Fanying , Yang Shuran , Zhang Mingzhu 
Published: June 2023

AbstractObjectiveIdiopathic gingival fibromatosis (IGF) is a rare heterogeneous disease that results in the progressive and diffuse hyperplasia of gingival tissues. MicroRNAs are implicated in the development and progression of various tumors. The present study aimed to explore the potential roles and mechanisms of miR‐148a‐3p in IGF.MethodsGingival fibroblasts (GFs) were transfected with miR‐148a‐3p mimics, miR‐148a‐3p inhibitors, or siNPTX1, and then, the proliferation and apoptosis of GFs and the expression of related genes were evaluated using Cell Counting Kit‐8 assays, 5‐ethynyl‐2′‐deoxyuridine assays, flow cytometry, reverse transcription‐quantitative polymerase chain reaction, and western blot analysis, respectively.ResultsmiR‐148a‐3p was highly expressed in GFs of IGF (IGF‐GFs) as compared with normal GFs (N‐GFs). Overexpression of miR‐148a‐3p promoted the proliferation and inhibited the apoptosis of N‐GFs, whereas downregulation of miR‐148a‐3p had the opposite effect in IGF‐GFs. Knockdown of NPTX1 reversed miR‐148a‐3p‐mediated effects in IGF‐GFs. Dual‐luciferase reporter assay confirmed that NPTX1 is a direct target of miR‐148a‐3p.ConclusionThese findings identify that miR‐148a‐3p could regulate cell proliferation and apoptosis by targeting NPTX1, providing new insights for the further study of the molecular mechanism and treatment of IGF.
Effects of low nitrogen supply on biochemical and physiological parameters related to nitrate and water, involving nitrate transporters and aquaporins in Citrus macrophylla
Authors: Garcia‐Gomez P. , Olmos‐Ruiz R. , Nicolas‐Espinosa J. , Carvajal M. 
Published: June 2023

Abstract
A reduction in chemical N‐based fertillizer was investigated in Citrus plants. As N and water uptake are connected, the relationship between the physiological response to reductions in N was studied in relation to N metabolism and water.
We examined the response of new and mature leaves and roots of Citrus macrophylla, grown under controlled conditions, and given different concentrations of N: 16, 8 or 4 mM. Differences in growth and development were determined for biochemical (mineral content, photosynthetic pigments, proteins and nitrate and nitrite reductase activity), physiological (photosynthesis and transpiration), and molecular (relative expression of nitrate transporters and aquaporins) parameters.
Only plants given 4 mM N showed a reduction in growth. Although there were changes in NR activity, protein synthesis, and chlorophyll content in both 8 and 4 mM N plants that were highly related to aquaporin and nitrate transporter expression.
The results revealed new findings on the relationship between aquaporins and nitrate transporters in new leaves of Citrus, suggesting a mechanism for ensuring growth under low N when new tissues are being formed.
“Rear bias” in spatial auditory perception: Attentional and affective vigilance to sounds occurring outside the visual field
Authors: Olszanowski Michal , Frankowska Natalia , Tołopiło Aleksandra 
Published: June 2023

AbstractPresented studies explored the rear bias phenomenon, that is, the attentional and affective bias to sounds occurring behind the listener. Physiological and psychological reactions (i.e., fEMG, EDA/SCR, Simple Reaction Task–SRT, and self‐assessments of affect‐related states) were measured in response to tones of different frequencies (Study 1) and emotional vocalizations (Study 2) presented in rear and front spatial locations. Results showed that emotional vocalizations, when located in the back, facilitate reactions related to attention orientation (i.e., auricularis muscle response and simple reaction times) and evoke higher arousal—both physiological (as measured by SCR) and psychological (self‐assessment scale). Importantly, observed asymmetries were larger for negative and threat‐related signals (e.g., anger) than positive/nonthreatening ones (e.g., achievement). By contrast, there were only small differences for the relatively higher frequency tones. The observed relationships are discussed in terms of one of the postulated auditory system's functions, which is monitoring of the environment in order to quickly detect potential threats that occur outside of the visual field (e.g., behind one's back).
Feasibility evaluation of two novel systems for the automated preparation and extended storage of DMSO cryopreserved platelets
Authors: Dumont Larry J. , Wolfe Brian , Leite Caroline , Moss Raymond , Wegener Christopher , Thompson Kyle , Min Kyungyoon 
Published: June 2023

AbstractBackgroundManufacturing methods for dimethyl sulfoxide (DMSO)‐cryopreserved platelets (CPPs) are manual and labor intensive. Thawing and prepare‐for‐transfusion steps are in an open system that requires transfusion within 4 h. A fill‐and‐finish system (CUE) can automate the manufacturing process. A newly configured bag system allows freezing, thawing, and use of resuspension solutions while maintaining the functionally closed system, and extending the post‐thaw shelf life beyond 4 h. Our objective is to evaluate the feasibility of the CUE system and the functionally closed bag system.Study design and MethodsDMSO was volumetrically added to double‐dose apheresis platelets, concentrated, and delivered to a 50‐ or 500‐mL ethylene‐vinyl acetate (EVA) bag by the CUE (n = 12). The functionally closed bag system contained 25 mL platelet additive solution 3 (PAS‐3) in a 50‐mL EVA bag. Control CPP (n = 2) were manually prepared. PAS‐3 and CPP were thawed together. CPP were stored up to 98 h (20–24°C) and tested using a standard assay panel.ResultsCUE prepared CPP met the design targets: volume, platelet content, and DMSO concentration. CUE CPP P‐selectin was high. CD42b, phosphatidylserine (PS) expression, and live cell percentage were favorable compared to controls and favorably maintained over storage. The thrombin generation potency was slightly reduced compared to controls. The 50 mL EVA bag maintained pH for up to 30 h, and the 500 mL EVA bag beyond 76 h.DiscussionThe CUE system presents a technically feasible method to prepare CPP. A functionally closed bag system with resuspension solution was successful and can extend the post‐thaw storage time of CPP.
Tolerability and the effect on skin Staphylococcus pseudintermedius density of repeated diluted sodium hypochlorite (bleach) baths at 0.005% in healthy dogs
Authors: Banovic Frane , Reno Lisa , Lawhon Sara D. , Wu Jing , Hoffmann Aline Rodrigues 
Published: June 2023

AbstractBackgroundDilute sodium hypochlorite (bleach) baths at 0.005% concentration twice weekly have been shown to markedly reduce the severity of atopic dermatitis in children, yet no tolerability and efficacy data are available for this treatment in dogs.ObjectivesTo determine the local tolerability and the longitudinal effect on the density of Staphylococcus pseudintermedius of repeated diluted bleach baths on healthy dog skin.AnimalsFour healthy hound cross‐bred dogs.MethodsBleach baths (0.005%; twice weekly for 15 min) were applied to four healthy hound cross‐bred dogs over four weeks (eight baths). Local tolerability was assessed for axillae, abdomen and legs by an investigator before, immediately after and 24 h after each bath. The longitudinal effect on density of S. pseudintermedius from axillae and groin was analysed through quantitative PCR before treatment [at Day (D)‐7 and ‐3], during treatment on D4, D11 and D25, and on D30.ResultsThere was no erythema or scaling after the baths in any dog. Copy numbers of S. pseudintermedius in axillae, groin and both (axillae and groin together) were not significantly different at any time point during the study.Conclusions and Clinical RelevanceRepeated 0.005% hypochlorite bleach baths over four weeks were safe and well‐tolerated in healthy dogs without significant changes in the density of S. pseudintermedius.
Treatment of canine spontaneous chronic corneal epithelial defects (SCCEDs) with corneal thermal cautery: A retrospective study of 89 cases
Authors: Landrevie Clara , Marchal Julie , Robin Marie‐Claire , Douet Jean‐Yves 
Published: June 2023

AbstractPurposeTo evaluate the clinical course and outcome of canine spontaneous chronic corneal epithelial defects (SCCEDs) treated with a combination of cotton‐tip epithelial debridement (ED) and corneal thermal cautery (CTC), with or without diamond burr debridement (DBD).MethodsRetrospective medical record review was used to identify dogs treated for SCCEDs at the Veterinary School of Toulouse between 2001 and 2021. The variables identified included signalment, history, clinical findings, previous treatments, and outcomes. Surgery was performed under manual restraint after topical anesthesia of the cornea. The outcome endpoints included healing, clearing of the cornea and complications.ResultsSeventy‐seven dogs (89 eyes) from 28 different breeds fulfilled the inclusion criteria. The mean age was 8.78 years. The overall success rate after one procedure was 65.1% with a mean healing time of 15.4 days, but 21 eyes lost to follow‐up after the initial treatment, were not included in calculations. There was no significant effect of age, eye, brachycephalic conformation and previous treatments. No significant differences in healing rates were found between groups that received DBD or not. Fifteen eyes (22.7%) underwent a second procedure. Complications (corneal pigmentation, uveitis, corneal bullae, and corneal infection) were observed in 15 cases (22%), with only two cases of complicating melting ulcer.ConclusionsCTC is a safe and effective treatment for SCCEDs in dogs and can be performed without general anesthesia. Additional DBD does not bring any significant value to CTC in SCCEDs. CTC could be interesting in cases where other procedures are less desirable.