bluuebunny/crossref_metadata_2025_split huggingface dataset:
Recombinant perlecan domain V covalently immobilized on silk biomaterials via plasma immersion ion implantation supports the formation of functional endothelium
Authors: Lau Kieran , Fu Lu , Zhang Anyu , Akhavan Behnam , Whitelock John , Bilek Marcela M. , Lord Megan S. , Rnjak‐Kovacina Jelena 
Published: March 2023

AbstractStrategies to promote rapid formation of functional endothelium are required to maintain blood fluidity and regulate smooth muscle cell proliferation in synthetic vascular conduits. In this work, we explored the biofunctionalization of silk biomaterials with recombinantly expressed domain V of human perlecan (rDV) to promote endothelial cell interactions and the formation of functional endothelium. Perlecan is essential in vascular development and homeostasis and rDV has been shown to uniquely support endothelial cell, while inhibiting smooth muscle cell and platelet interactions, both key contributors of vascular graft failure. rDV was covalently immobilized on silk using plasma immersion ion implantation (PIII), a simple one‐step surface treatment process which enables strong immobilization in the absence of chemical cross‐linkers. rDV immobilization on surface‐modified silk was assessed for amount, orientation, and bio‐functionality in terms of endothelial cell interactions and functional endothelial layer formation. rDV immobilized on PIII‐treated silk (rDV‐PIII‐silk) supported rapid endothelial cell adhesion, spreading, and proliferation to form functional endothelium, as evidenced by the expression of vinculin and VE‐cadherin markers. Taken together, the results provide evidence for the potential of rDV‐PIII‐silk as a biomimetic vascular graft material.
Does corporate social responsibility play a moderating role in trading silence prior to bad news earnings announcements?
Authors: Chakraborty Atreya , Gao Lucia Silva , Kim Sangwan , Liu Rongbing 
Published: March 2023

AbstractWe examine whether corporate social responsibility (CSR) plays a role in moderating trading silence prior to bad news earnings announcements. Unusually low trading volume before the public release of negative earnings information constrains price discovery. We find that unusually low trading volume prior to earnings announcements is less pronounced for firms with a high level of CSR activities. We also find that this effect is stronger before bad news earnings announcements than good news earnings announcements. These findings are robust to various alternative research design choices and to the endogeneity concern between CSR and trading activity. Taken together, our study demonstrates that CSR plays a moderating role in trading silence by improving firms’ pre‐disclosure business and information environments.
Enhanced excretion of recombinant cyclodextrin glucanotransferase and cell stability of immobilized recombinant Escherichia coli by reducing tryptone concentration
Authors: Man Rohaida Che , Manas Nor Hasmaliana Abdul , Illias Rosli Md , Mudalip Siti Kholijah Abdul , Sulaiman Siti Zubaidah , Masngut Nasratun 
Published: March 2023

AbstractBACKGROUNDRecombinant protein excretion has become a mainstream strategy in reducing downstream processing costs. However, recombinant protein excretion is often bottlenecked by cell lysis and plasmid stability. In the present study, recombinant Escherichia coli cell immobilization was performed on a hollow fiber membrane. Tryptone concentration in the expression medium (Super Optimal Broth), which was used as a nitrogen source, was varied between 5 and 20 g L−1 to enhance the excretion of recombinant cyclodextrin glucanotransferase (CGTase), plasmid stability, and resistance of cell lysis.RESULTSThe immobilized cells with 5 g L−1 concentration of tryptone improved the plasmid stability with 119% improvement and 69% reduction of cell lysis without remarkably altering the excretion of CGTase compared with the tryptone concentration of 20 g L−1. The immobilized cells showed a 2‐fold increase in excretion of CGTase, a 45% reduction in cell lysis, and a 172% gain in plasmid stability in comparison with the free cells. Moreover, the doubling time increased to 58 and 5 h for the immobilized and free cells, respectively. The immobilized cells recorded 2301.62 U mL−1 of cumulative CGTase activity through seven fermentation cycles using the untreated membrane, marking their excellent reusability.CONCLUSIONThis new technique of recombinant protein expression utilizing an immobilized cell system under low tryptone concentration is an outstanding approach to improve recombinant CGTase excretion and plasmid stability with low cell lysis. © 2023 Society of Chemical Industry (SCI).
Accessorizing development: Fundraising bracelets for International Development as a New Development Responsibility
Authors: Hill‐Tout Kimberly N. , Hawkins Roberta 
Published: March 2023

AbstractIndividuals in the Global North wishing to support people in the Global South can purchase a fundraising bracelet in order to ‘change lives’. This article examines the use of fundraising bracelets for International Development (FBID) as a form of ‘New Development Responsibility’. We illustrate through a database and critical discourse analysis that FBID campaigns are a distinctly feminized version of ID aid where women are engaged as consumers, campaign founders and artisans. Our analysis of these different roles suggest that FBID represent a specifically feminized version of the ‘White Saviour Complex’ requiring further exploration, particularly around the ‘accessorization’ of ID.
Long‐term follow‐up in preschool children after radiofrequency catheter ablation of arrhythmias
Authors: Svintsova Liliya I. , Krivolapov Sergey N. , Dzhaffarova Olga Y. , Plotnikova Irina V. 
Published: March 2023

AbstractBackgroundRadiofrequency ablation (RFA) is the standard method of treatment for tachyarrhythmias in school children, and it leads to complete recovery in children without structural heart disease. However, RFA in young children is limited by the risk of complications and unstudied remote effects of radiofrequency lesions.ObjectiveTo present the experience of RFA of arrhythmias and the results of follow‐up of younger children.Materials and MethodsRFA procedures (n = 255) were performed in 209 children with arrhythmias from 0 to 7 years old. The arrhythmias were presented with atrioventricular reentry tachycardia with Wolff‐Parkinson‐White (WPW) syndrome (56%), atrial ectopic tachycardia (21.5%), atrioventricular nodal reentry tachycardia (4.8%), and ventricular arrhythmia (17.2%).ResultsThe overall effectiveness of RFA, considering the repeated procedures performed due to the primary ineffectiveness and recurrencies, was 94.7%. There was no mortality associated with RFA in patients, including young patients. All cases of “major” complications are associated with RFA of the left‐sided accessory pathway and tachycardia foci and are represented by the mitral valve damage in three patients (1.4%). Tachycardia and preexcitation recurred in 44 (21%) patients. There was a correlation between recurrences and parameters of RFA (odds ratio 0.894; 95% confidence interval: 0.804–0.994; p = .039). Reducing the maximum power of effective applications in our study increased the risk of recurrence.ConclusionThe use of the minimum effective parameters of RFA in children reduces the risk of complications, but increases arrhythmia recurrence rate.
Micro‐structural study of crystals and glassy matrix of black glazed wares from Jian kilns, China (Song Dynasty, AD 960–1279)
Authors: Qian Cheng , Li Qiang , Wang Fen , Zhu Jianfen , Zhao Jing , Yang Chi , Wang Tian 
Published: March 2023

AbstractBlack glazed wares (Wujin wares) and hare fur's decorated black glazed wares (HF wares) are ones of typical production of Jian kilns, China during Song Dynasty. In this work, Wujin wares and HF wares excavated in Jian kiln complex (Yulinting kilns, Daluhoumen kilns and Luhuaping kilns) were systematically analyzed by optical microscope, X‐ray fluorescence spectroscopy and micro‐Raman spectroscopy. The results show that the index of glassy polymerization (Ip) of Wujin wares is smaller than black areas of HF wares, indicating the higher firing temperature used for HF wares. Several pseudobrookite, rutile, hematite and quartz crystals were detected in glazes of Wujin wares while a great number of ɛ‐Fe2O3 crystals was observed in brown stripes of HF wares. Besides, wares in different kiln sites present obviously differences in bubble distribution and glassy structure, indicating a lower firing temperature used for Yulinting kilns than those of Daluhoumen kilns and Luhuaping kilns.
Juvenile Nasopharyngeal Angiofibroma: Outcomes Analysis Based Upon Hospital Volume
Authors: Adil Eelam A. , Francisco Sarah , Morgan Ella , Kawai Kosuke , Cunningham Michael J. 
Published: March 2023

ObjectiveTo evaluate the management of juvenile nasopharyngeal angiofibroma (JNA) from a national perspective with outcomes comparison based on hospital volume.Study DesignTen‐year Pediatric Health Information Systems (PHIS) data analysis.MethodsThe PHIS database was queried for the diagnosis of JNA. Data regarding demographics, surgical approach, embolization, length of stay, charges, readmission, and revision surgery was collected and analyzed. Hospitals were classified as low volume if fewer than 10 cases and high volume if greater than or equal to 10 cases during the study period. A random effects model compared outcomes based on hospital volume.ResultsA total of 287 JNA patients were identified with a mean age of 13.8 (± 2.7) years. Nine hospitals were classified as high volume, accounting for a total of 121 patients. The mean length of hospitalization, blood transfusion rate, and 30‐day readmissions did not differ significantly by hospital volume. Patients cared for at high‐volume institutions were less likely to require postoperative mechanical ventilation (8.3% vs. 25.0%; adjusted RR = 0.32; 95% CI 0.14–0.73; p < 0.01) or return to the operating room for residual disease than patients admitted to low‐volume hospitals (7.4% vs. 20.5%; adjusted RR = 0.38; 95% CI 0.18–0.79; p = 0.01).ConclusionsThe management of JNA is complex from both an operative and perioperative management standpoint. Over the past decade, nearly half (42.2%) of JNA patients have been managed at nine institutions in the United States. These centers have significantly lower rates of postoperative mechanical ventilation and the need for revision surgery.Level of Evidence3 Laryngoscope, 133:3216–3220, 2023
Discharge Disposition After Head and Neck Reconstruction: Effect on Adjuvant Therapy and Outcomes
Authors: Dang Sophia , Patel Terral , Lao Isabella , Sridharan Shaum S. , Solari Mario G. , Kim Seungwon , Duvvuri Umamaheswar , Ferris Robert , Kubik Mark 
Published: March 2023

ObjectivesHead and neck cancer patients that require major reconstruction often have advanced‐stage disease. Discharge disposition of patients can vary and impact time to adjuvant treatment. We sought to examine outcomes in patients discharged to skilled nursing facilities (SNF) compared to those discharged home, including the impact on adjuvant therapy initiation and treatment package time (TPT).MethodsPatients with head and neck squamous cell carcinoma treated with surgical resection and microvascular free flap reconstruction from 2019 to 2022 were included. Retrospective review was conducted to evaluate the impact of disposition on time to radiation (RT) and TPT.Results230 patients were included, with 165 (71.7%) discharged to home and 65 (28.3%) discharged to SNF. 79.1% of patients were recommended adjuvant therapy. Average time to RT was 59 days for patients discharged to home compared to 70.1 days for patients discharged to SNF. Disposition was an independent risk factor for delays to starting RT (p = 0.03). TPT was 101.7 days for patients discharged to home versus 112.3 days for those who discharged to SNF. Patients discharged to SNF had higher rates of readmission (p < 0.005) compared to patients discharged home in an adjusted multivariate logistic regression.ConclusionsPatients discharged to an SNF had significantly delayed time to initiation of adjuvant treatment and higher rates of readmission. Timeliness to adjuvant treatment has recently been established as a quality measure, thus identifying delays to adjuvant treatment initiation should be a priority.Level of Evidence3 Laryngoscope, 133:2977–2983, 2023
The Effects of Nasal Surgery on Pulmonary Function: A Systematic Review and Meta‐Analysis
Authors: Baharmand Iman , Sheikh‐Oleslami Sara , Pascual Rodríguez Athenea , Hernaiz‐Leonardo Juan Carlos , Alim Bader M. , Javer Amin R. 
Published: March 2023

ObjectiveA deviated nasal septum (DNS) can result in an anatomical obstruction and impact lung function through prolonged suboptimal inspiration. Given the improvements in respiration reported by patients following septoplasty or septorhinoplasty (with or without inferior turbinate reduction), our study investigated the effect of these procedures on pulmonary function through a systematic review and meta‐analysis.Data SourcesMedline, Embase, Cochrane Databases, Web of Science, and Google Scholar.Review MethodsThe review was registered with PROSPERO [CRD42022316309]. The study population was composed of adult patients (18–65) who were symptomatic with confirmed DNS. Extracted outcomes (pre‐operative versus postoperative) included the six‐minute walk test (6MWT) and pulmonary function tests (FEV1, FVC, FEV1/FVC, FEF25‐75, PEF). Meta‐analyses were performed using a random‐effects model.ResultsThree studies included measures of the 6MWT in meters and all three found a statistically significant increase in the distance walked after surgery with a mean difference of 62.40 m (95% CI 24.79–100.00). Statistically significant improvements in PFT outcomes were observed with a standard mean difference of 0.72 for FEV1 (95% CI 0.31–1.13), 0.63 for FVC (95% CI 0.26–1.00), and 0.64 for PEF (95% CI 0.47–0.82). Of the twelve studies which measured PFT outcomes, six showed statistically significant improvements, three studies showed mixed results, and three studies found no difference in PFT outcomes between pre‐and post‐surgery testing.ConclusionsThe present study suggests that pulmonary function does improve after nasal surgery for DNS, but the high heterogeneity observed in the meta‐analyses indicates that the evidence supporting this conclusion is low. Laryngoscope, 133:2837–2845, 2023
Association Between Hearing Impairment and Incident Depression: A Nationwide Follow‐up Study
Authors: Kim Hye Jun , Jeong Seogsong , Roh Kyung Jin , Oh Yun Hwan , Suh Michelle J. 
Published: March 2023

BackgroundAccumulating evidence suggests that hearing impairment is associated with the onset of depression. However, large‐scale epidemiological studies are required to define this association more clearly. We aimed to investigate the risk of new‐onset depression in Korean older adults with and without hearing impairment.MethodsFrom the National Health Insurance Service‐Senior Cohort, which is a retrospective‐prospective hybrid database, we analyzed data for 254,466 older adults enrolled in the Korea National Health Insurance Service‐Senior Cohort who underwent at least one health screening between 2003 and 2019. A Cox proportional hazards regression model was used to evaluate the association between hearing impairment and the risk of incident depression, which was presented as adjusted hazard ratios (aHR) with 95% confidence intervals (CIs). All participants were followed up until the date of incident depression, death, or December 31, 2019.ResultsDuring 3,417,682 person‐years of follow‐up investigation, hearing impairment was associated with a higher risk of incident depression (vs. no hearing impairment) in the final adjusted model (aHR, 1.11; 95% CI, 1.01–1.21; p = 0.033). Stratified analyses revealed a significant interaction among age, hearing impairment, and the risk of depression. Participants aged <65 years had a higher risk of depression (aHR, 1.29; 95% CI, 1.12–1.50; p < 0.001) than those aged 65 or above (aHR, 1.15; 95% CI, 1.01–1.30; p = 0.032).ConclusionsHearing impairment is independently associated with a higher risk of depression among older adults. The prevention and treatment of hearing impairment may aid in mitigating the risk of incident depression.Level of EvidenceLevel 3 Laryngoscope, 133:3144–3151, 2023
Enhanced Cell Penetration and Pluripotency Maintenance of hiPSCs in 3D Natural Chitosan Scaffolds
Authors: Tang Yuanzhang , Zhou Yang , Lin Guanyou , Zhang Miqin 
Published: March 2023

AbstractHuman‐induced pluripotent stem cells (hiPSCs) cultured in 3D matrices hold great promise in disease modeling, drug discovery, and tissue regeneration. Uniform cell distribution in a 3D structure is critical to the growth and function of hiPSCs, yet cell seeding in 3D matrices often remains superficial, leading to limited cell proliferation and compromised pluripotency. Here, an approach to improve cell penetration depth of hiPSCs in 3D scaffolds modified with hiPSCs conditioned medium (CM) is reported. It is shown that extracellular matrix components are successfully deposited onto the scaffold wall surface after CM treatment and promoted homogeneous cell adhesion during initial seeding. Compared to plain, unmodified scaffolds, the CM treated scaffold improves spatial cell distribution uniformity and upregulates pluripotency markers. Notably, the expression of 29 genes associated with 11 signaling pathways participated in the pluripotency maintenance of hiPSCs exhibits >2‐fold change in hiPSCs grown in the CM treated scaffolds than 2D counterparts, demonstrating that CM treated scaffolds can support a more primitive and undifferentiated phenotype of hiPSCs. This study introduces a simple and effective method to enhance cell penetration and maintain cell pluripotency in 3D matrices.
How Is Functional Neurological Disorder Managed in Australian Hospitals? A Multi‐Site Study Conducted on Acute Inpatient and Inpatient Rehabilitation Wards
Authors: Petrie Dharsha , Lehn Alexander , Barratt Jessica , Hughes Amy , Roberts Kathryn , Fitzhenry Sarah , Gane Elise 
Published: March 2023

AbstractBackgroundSuccessful management for functional neurological disorder (FND) requires multidisciplinary involvement starting with providing a definitive diagnosis.ObjectivesTo observe clinical management of patients with FND during hospital admission.MethodsA prospective observational study was conducted over six Australian hospitals over a 4‐month period. Data collected included patient demographics, communication of the diagnosis of FND, access to the multidisciplinary team, hospital length of stay (LOS), and emergency department (ED) presentations.ResultsA total of 113 patients were included. Median LOS was 6 (interquartile range, 3–14) days. Thirty‐five (31%) presented to ED with 9 (8%) re‐presenting two or more times after hospital discharge. Total hospital utilization cost was AUD$3.5million. A new diagnosis was made in 82 (73%) patients. Inpatient referrals were made to neurology (81, 72%), psychology (29, 26%), psychiatry (27, 24%), and physiotherapy (100, 88%). Forty‐four (54%) were not told of the diagnosis. Twenty (24%) did not have their diagnosis documented in their medical record. Of the 19 (23%) not reviewed by neurology on non‐neurosciences wards, 17 (89%) did not have their diagnosis communicated and 11 (58%) did not have it documented. Twenty‐five (42%) referred to neurology were not provided with a diagnosis.ConclusionsCurrent gaps in service provision during inpatient hospital admissions in Australia include low rates of communication of a diagnosis, particularly when patients are not located on a neurosciences ward, and limited and variable access to inpatient multidisciplinary teams. Specialized services are needed to improve education, clinical pathways, communication, and health outcomes while reducing healthcare system costs.
Immediate‐delayed lymphatic reconstruction after axillary lymph nodes dissection for locally advanced breast cancer‐related lymphedema prevention: Report of two cases
Authors: Brahma Bayu , Yamamoto Takumi , Agdelina Clarissa , Adella Devina , Putri Rizky Ifandriani , Hanifah Wardah , Sundah Vincentius Henry , Perdana Adhitya Bayu , Putra Mohammad Reka Ananda , Taher Akmal , Panigoro Sonar Soni 
Published: March 2023

AbstractApproximately 60%–70% of breast cancer patients in Indonesia are diagnosed in the locally advanced stage. The stage carries a higher risk of lymph node metastasis which increases susceptibility to lymph obstruction. Hence, breast cancer‐related lymphedema (BCRL) could present before axillary lymph node dissection (ALND). The purpose of this case report is to describe immediate‐delayed lymphatic reconstructions with lymphaticovenous anastomosis in two subclinical lymphedema cases that present before ALND. There were 51 and 58 years old breast cancer patients with stage IIIC and IIIB, respectively. Both had no arm lymphedema symptoms, but arm lymphatic vessel abnormalities were found during preoperative indocyanine green (ICG) lymphography. Mastectomy and ALND were performed and proceeded with lymphaticovenous anastomoses (LVA) in both cases. One LVA at the axilla (isotopic) was done in the first patient. On the second patient, 3 LVAs at the affected arm (ectopic) and 3 isotopic LVAs were created. The patients were discharged on the second day without complications during the follow‐up. The intensity of dermal backflow was reduced, and no subclinical lymphedema progression occurred during 11 and 9 months follow‐up, respectively. Based on these cases, BCRL screening might be recommended for the locally advanced stage before cancer treatment. Once diagnosed, immediate lymphatic reconstruction after ALND should be recommended to cure or prevent BCRL progression.
Mitochondrial dysfunction caused by SIRT3 inhibition drives proinflammatory macrophage polarization in obesity
Authors: Zhou Qing , Wang Yuyan , Lu Zongshi , Wang Bowen , Li Li , You Mei , Wang Lijuan , Cao Tingbing , Zhao Yu , Li Qiang , Mou Aidi , Shu Wentao , He Hongbo , Zhao Zhigang , Liu Daoyan , Zhu Zhiming , Gao Peng , Yan Zhencheng 
Published: March 2023

AbstractObjectiveMetabolic reprogramming is a main feature of proinflammatory macrophage polarization, a process that leads to inflammation in dysfunctional adipose tissue. Therefore, the study aim was to explore whether sirtuin 3 (SIRT3), a mitochondrial deacetylase, participates in this pathophysiological process.MethodsMacrophage‐specific Sirt3 knockout (Sirt3‐MKO) mice and wild‐type littermates were treated with a high‐fat diet. Body weight, glucose tolerance, and inflammation were evaluated. Bone marrow–derived macrophages and RAW264.7 cells were treated with palmitic acid to explore the mechanism of SIRT3 on inflammation.ResultsThe expression of SIRT3 was significantly repressed in both bone marrow–derived macrophages and adipose tissue macrophages in mice fed with a high‐fat diet. Sirt3‐MKO mice exhibited accelerated body weight and severe inflammation, accompanied with reduced energy expenditure and worsened glucose metabolism. In vitro experiments showed that SIRT3 inhibition or knockdown exacerbated palmitic acid–induced proinflammatory macrophage polarization, whereas SIRT3 restoration displayed opposite effects. Mechanistically, SIRT3 deficiency resulted in hyperacetylation of succinate dehydrogenase that led to succinate accumulation, which suppressed the transcription of Kruppel‐like factor 4 via increasing histone methylation on its promoter, thus evoking proinflammatory macrophages.ConclusionsThis study emphasizes an important preventive role of SIRT3 in macrophage polarization and implies that SIRT3 is a promising therapeutic target for obesity.