bluuebunny/crossref_metadata_2025_split huggingface dataset:
A fractional-order creep constitutive model considering the viscoelastic - viscoplastic coexistence mechanism
Authors: Zhao Jia , Zhao Weigang , Xie Kaize , Yang Yong 
Published: June 2023

Abstract
In order to improve the accuracy and universality of the nonlinear viscoelastic-plastic mechanical behavior characterization method of asphalt mixture, a new criterion for the division of creep process of materials is established based on the strain yield characteristics, and the coexistence mechanism of viscoelastic-viscoplastic strain is proposed in the subsequent yield stage; Then, a novel viscoelastic element in the form of parallel connection of two fractional viscoelastic elements is established based on the theory of fractional calculus, and its mathematical equation is derived; With the novel viscoelastic element, a constitutive model characterizing the whole process of creep of asphalt mixture is established, and its analytical expression is derived. The laboratory short-term creep test of Cement and Asphalt Mortar (CA mortar) and the simulation test data of asphalt mixture from the references were used to verify the constitutive model. The results show that the constitutive model has good adaptability to the creep characteristics of different types of asphalt mixture under different stress levels.So, the model can used to provide a theoretical reference for the study of the mechanical behavior characterization of asphalt materials.
Hypertonic saline induces inflammation in human macrophages through the NLRP1 inflammasome
Authors: Sposito Francesca , Northey Sarah , Charras Amandine , McNamara Paul , Hedrich Christian 
Published: June 2023

Abstract
Nebulized hypertonic saline (3–7%) is commonly used to increase mucociliary clearance in patients with chronic airway disease and/or virus infections. However, altered salt concentrations may contribute to inflammatory responses. The aim of this study was to investigate whether 500mM NaCl (3%) triggers inflammation in human macrophages and identify the molecular mechanisms involved. NaCl induced pyroptosis, IL-1β, IL-18 and ASC speck release was measured in primary human monocyte-derived macrophages. Treatment with the recombinant IL-1 receptor antagonist anakinra or the NLRP3 inhibitor MCC950 did not affect NaCl-mediated inflammasome assembly. Knock-down of NLRP1 expression, but not of NLRP3 and NLRC4, reduced NaCl induced pyroptosis, proinflammatory cytokine and ASC speck release from human THP-1-derived macrophages. Data from this study suggest that 3% NaCl induced inflammatory responses in human macrophages depend on NLRP1 and inflammasome assembly. Targeting inflammation in addition to inhalation with hypertonic saline may benefit patients with inflammatory airway disease.
Scale-selective identification and assessment of global eddies from satellite data
Authors: Yang Yikai , Zeng Lili , Wang Qiang 
Published: June 2023

Abstract
Automatic ocean eddy identification algorithms are crucial for global eddy research. In this study, a scale-selective eddy identification algorithm (SEIA) that features improvements in the detection and tracking processes is presented for the global ocean based on closed sea level anomalies. First, the previous strategy of using thresholds to define eddy boundaries is replaced with a scale-selective scheme, which restricts the numbers of upper and lower grid points based on the data resolution and eddy spatial scale. Under such conditions, eddy boundaries that are overestimated will be flexibly removed. Furthermore, an effective overlap scheme is used to track eddies by calculating the intersection ratio of time-step-successive eddies. The SEIA generates approximately 1.6 million anticyclonic eddies and 1.5 million cyclonic eddies by the satellite altimetry product from the French Archiving, Validation, and Interpretation of Satellite Oceanographic Data (AVISO) over a 29-year period (1993-2021; https://doi.org/10.11922/sciencedb.o00035.00004). Assessments of the global distribution of eddies, eddy propagation speed, eddy path and evolution characteristics, and observation-based eddy hydrological conditions verify the validity of the SEIA.
COVID-19 and flu, exploring the infection of virus, the safety and attitudes of vaccination in pediatric patients with rheumatic diseases
Authors: Yi Cuili , Zheng Xiaohuang , Lin Kezhao , Xiao Jihong 
Published: June 2023

Abstract
Background
Patients with rheumatic diseases have an increased burden of infections. Immunization is recognized as being one of the most efficient means to prevent infections. However, many patients refuse or hesitate to be vaccinated mainly due to safety concerns. We performed this study to explore the infection of COVID-19 and influenza, the safety and attitudes regarding inactivated COVID-19 vaccination and influenza vaccination in pediatric rheumatic patients.
Methods
We conducted a retrospective survey using a questionnaire from November 1, 2021 to February 28, 2022. Pediatric rheumatic patients with inactive disease received inactivated COVID-19 vaccine at the age of 3–18 years old were recruited. Demographic data and vaccination adverse events (AEs) were collected. All parents of patients were telephone-interviewed in May 2023 regarding COVID-19 infection, influenza infection and influenza vaccination of their children in the past one year. Self-reported disease flares that occurred after infection and vaccination, as well as reasons for non-vaccination were recorded.
Results
A total of 192 patients were recruited. Local AEs occurred in 11.5% and 10.1% of patients after the first and the second dose of COVID-19 vaccination; the percentages were 8.9% and 6.3% for systemic AEs. Three (1.6%) patients experienced original disease flare. In total, 177 parents responded the telephone questionnaire, among which, 101 (57.1%) of their children had COVID-19 infection. All had mild symptoms and 8 patients experienced disease flares. Thirty-two (18.1%) patients had influenza infection. All experienced mild symptoms except one patient was hospitalization. One patient experienced a disease flare. Seventeen (9.6%) patients were vaccinated against influenza, none of them experienced serious AEs or disease flare. The main reasons for non-vaccination of influenza were the fear of AEs and disease flare (n = 60, 37.5%) and not receiving enough information about influenza vaccination from their doctors (n = 84, 52.5%). Ninety-six (60%) parents whose children were non-vaccination responded that would vaccinate their children if advised to by a rheumatologist.
Conclusions
These findings confirm on the safety of inactivated COVID-19 vaccination and inactivated influenza vaccination in pediatric rheumatic patients. Rheumatologists should take more responsibility in vaccination education of pediatric rheumatic patients.
Protein-mediated folding of the genome is essential for site-specific integration of foreign DNA into CRISPR loci
Authors: Santiago-Frangos Andrew , Henriques William , Wiegand Tanner , Gauvin Colin , Buyukyoruk Murat , Neselu Kasahun , Eng Edward , Lander Gabriel , Wilkinson Royce , Graham Ava , Wiedenheft Blake 
Published: June 2023

Abstract
Bacteria and archaea acquire resistance to viruses and plasmids by integrating fragments of foreign DNA into the first repeat of a CRISPR array. However, the mechanism of site-specific integration remains poorly understood. Here, we determine a 560 kDa integration complex structure that explains how Cas (Cas1-2/3) and non-Cas proteins (IHF) fold 150 base-pairs of host DNA into a U-shaped bend and a loop that protrude from Cas1-2/3 at right angles. The U-shaped bend traps foreign DNA on one face of the Cas1-2/3 integrase, while the loop places the first CRISPR repeat in the Cas1 active site. Both Cas3s rotate 100-degrees to expose DNA binding sites on either side of the Cas2 homodimer, that each bind an inverted repeat motif in the leader. Leader sequence motifs direct Cas1-2/3-mediated integration to diverse repeat sequences that have a 5’-GT.
Implementation of different relationship estimate methodologies in Breeding Value prediction in kiwiberry (Actinidia arguta)
Authors: Mertten Daniel , Baldwin Samantha , Cheng Canhong H. , McCallum John , Thomson Susan , Ashton David T. , McKenzie Catherine , Lenhard Michael , Datson Paul M. 
Published: June 2023

Abstract
In dioecious crops such as Actinidia arguta(kiwiberries), some of the main challenges when breeding for fruit characteristics is the selection of potential male parents and the long juvenile period. Currently, breeding values of male parents are estimated through progeny tests, which makes the breeding of new kiwiberry cultivars time-consuming and costly. The application of Genomic Best Linear Unbiased Prediction (BLUP), which does not rely exclusively on information of relatives, would allow direct estimation of sex-related traits and speed up kiwiberry breeding. Owing to the high cost of genome sequencing, it is important to understand the effects of different sources of relationship matrices on estimating breeding values across a breeding population. In this study, we used a linear mixed model approach to estimate narrow sense heritability for one vine-related trait and five fruit-related traits for two incomplete factorial crossing designs. We obtained BLUPs for all genotypes, taking into consideration whether the relationship was pedigree-based or marker-based. Because of the increasing implementation of genomic selection in crop breeding, we compared the effects of incorporating different sources of information in building relationship matrices and ploidy levels on the accuracy of BLUPs, heritability and predictive ability. As kiwiberries are autotetraploids, multivalent chromosome formation and occasionally double reduction can occur during meiosis, and this can affect the accuracy of prediction. We demonstrate that the accuracy of BLUPs of male siblings, without phenotypic observations, strongly improved when a tetraploid marker-based relationship matrix was used rather than parental BLUPs and female siblings with phenotypic observations.
Lysozyme-Derived Peptides as a Novel Nutra-Preservative to Control Some Food Poisoning and Food Spoilage Microorganisms
Authors: Abdou Adham M. , Awad Dina A. B. 
Published: June 2023

Abstract
Food-borne illnesses and microbial food contamination are crucial concerns and still issues of major worldwide concern. Lysozyme (Lz) is an active protein against Gram-positive bacteria through its muramidase lytic activity, however, several authors could identify some antimicrobial peptides derived from native Lz that have an exaggerated and broad-spectrum antibacterial activity. Therefore, a lysozyme peptides preparation (LzP) is developed in order to broaden the Lz spectrum. In this work, we investigated the potential efficacy of LzP as a novel Nutra-preservative (food origin) agent against some pathogenic and spoilage bacteria. Our results showed that LzP demonstrated only 11% of the lysozyme lytic activity. However, LzP exhibited strong antibacterial activity against Escherichia coli, Salmonella enteritidis, and Pseudomonas species, while Salmonella typhi and Aeromonas hydrophila exhibited slight resistance. Despite the lowest LzP concentration (0.1%) employed, it performs stronger antibacterial activity than weak organic acids (0.3%). Interestingly, the synergistic multi-component formulation (LzP, glycine, and citric acid) could inhibit 6 log10 cfu/ml of E. coli survival growth. The effect of heat treatment on LzP showed a decrease in its antibacterial activity at 5 and 67% by boiling at 100ºC/30 min, and autoclaving at 121 ºC/15 min; respectively. On the other hand, LzP acquired stable antibacterial activity at different pH values (4–7). In conclusion, LzP would be an innovative, natural, and food-origin preservative to control the growth of food poisoning and spoilage bacteria in food.
Identification of novel inhibitors from Urtica spp against MDAMB-231 targeting JAK 2 receptor for breast cancer therapy
Authors: Upreti Shobha , Muduli Kartik , Pradhan Jagannath , Elangovan Selvakumar , Samant Mukesh 
Published: June 2023

Abstract
Breast cancer is the most prevalent form of cancer in women globally, and MDA-MB-231 or TNBC (Triple-negative breast cancer) is its aggressive type since it lacks the usual targets. JAK2/STAT3 pathway can be an important lead in anticancer drug discovery, as restraining the downstream signalling of this pathway results in the induction of cell apoptosis. Moreover, various limitations associated with chemotherapy are the reason to find an alternative herbal-based therapy. For this study, we collected Urtica dioica, and U. parviflora from different regions of Uttarakhand, followed by preparation of their leaf and stem extracts in different solvents. The GC-MS analysis of these extracts revealed a total of 173 compounds to be present in them. Further, by molecular docking approach, we studied the interaction between these compounds and JAK2, and 12 major compounds with better binding energy than the control Paclitaxel were identified. In addition, the selected hits were also reported to display better pharmacokinetic properties. The anticancer potential of these extracts was also evaluated by in vitro approach in the MDA-MB-231 cell line, and both extracts displayed significant anticancer activity. Hence, the findings in our study can be crucial in the area of herbal-based target-specific drug development against breast cancer.
An energetics tale of the 2022 mega-heatwave over central-eastern China
Authors: Zhang Tuantuan , Deng Yi , Yang Song , Zhang Henian , Dai Yongjiu , Chen Junwen 
Published: June 2023

Abstract
It remains a major challenge to attribute heatwave’s lifecycle characteristics quantitatively to interwoven atmospheric and surface actions. By constructing a process-resolving, energetics-based attribution framework (PREAF), here we quantitatively delineate the lifecycle of the unprecedented 2022 mega-heatwave over central-eastern China from a local energetics perspective. It is found that the cloudlessness induced radiative heating and atmospheric dynamics dominate the total energy buildup during the developing stage, while the land-atmosphere coupling and atmospheric horizontal advection act most effectively to sustain and terminate the heatwave, respectively. A reduction in anthropogenic aerosols provides a persistent positive contribution during the event, suggesting that pollution mitigation measures may actually increase the amplitudes of future heatwaves. With the PREAF, initial efforts are made to unravel culprits in a model’s sub-seasonal prediction of this mega-heatwave, demonstrating the framework’s potential for efficiently detecting the origins of climate extremes and quantitatively assessing the impacts of mitigation policies for sustainable development.
Anti-Müllerian Hormone: a novel biomarker for aggressive prostate cancer? Emerging evidence from a prospective study of radical prostatectomies
Authors: Kontogiannis Stavros , Markantes Georgios , Stamou Maria , Tsagkarakis Michail , Mamali Irini , Giannitsas Konstantinos , Perimenis Petros , Georgopoulos Neoklis , Athanasopoulos Anastasios 
Published: June 2023

Abstract
PURPOSE Prostate cancer patients are a heterogeneous group concerning the aggressiveness of the disease. The relationship of steroid hormones with the aggressiveness of prostate cancer is unclear. It is known that the Anti-Müllerian Hormone (AMH), inhibits prostate cancer cell lines in vitro. The aim of this study is to investigate the relationship of AMH and steroid hormones with the aggressiveness of prostate cancer.METHODS This was a prospective study of consecutive radical prostatectomy patients. We measured the following hormones: total testosterone, sex hormone binding globulin, albumin, luteinizing hormone, follicle stimulating hormone, estradiol, dehydroepiandrosterone sulfate, androstenedione and AMH. The minimum follow-up after radical prostatectomy was 5 years. For the aggressiveness of prostate cancer, we considered the following 3 variables: post-operative Gleason Score (GS) ≥ 8, TNM pΤ3 disease and PSA biochemical recurrence (BCR).RESULTS In total, 91 patients were enrolled. The mean age and PSA was 64.8 years and 9.3 ng/dl, respectively. The median post-operative GS was 7. Low AMH blood levels were correlated with higher post-operative GS (p = 0.001), as well as with PSA BCR (p = 0.043). With pT3 disease, only albumin was (negatively) correlated (p = 0.008). ROC analysis showed that AMH is a good predictor of BCR (AUC 0.646, 95% CI 0.510–0.782, p = 0.043); a cut-off value of 3.06 ng/dl had a positive prognostic value of 71.4% and negative prognostic value of 63.3% for BCR. Cox regression analysis showed that AMH is a statistically significant and independent prognostic marker for BCR (p = 0.013). More precisely, for every 1 ng/ml of AMH rise, the probability for PSA BCR decreases by 20.8% (HR = 0.792). Moreover, in Kaplan-Meier analysis, disease-free survival is more probable in patients with AMΗ ≥ 3.06 ng/ml (p = 0.004).CONCLUSIONS Low AMH blood levels were correlated with aggressive prostate cancer in this radical prostatectomy cohort of patients. Therefore, AMH could be a prognostic biomarker for the aggressiveness of the disease.
Autoinflammatory Gene Mutations Associated with Eosinophilia and Asthma
Authors: alotaibi bashayr , Lopez Raquel , Garrido Carmen Venegas , Gonzalez Lucia , Khalidi Nader , Nair Parameswaran 
Published: June 2023

Abstract
Background
Respiratory conditions, such as asthma, are infrequently associated with auto-inflammatory diseases. We describe five patients with uncontrolled respiratory symptoms that were seen at St. Joesph’s Healthcare in Hamilton for severe asthma management diagnosed with rare autoinflammatory conditions using genetic molecular analysis.
Case presentation:
Five patients are included in this case series. Gene mutations associated with familial Mediterranean fever (FMF), Yao syndrome, Cryopyrin-associated periodic syndrome (CAPS), and Majeed syndrome were considered to explain partly the patient’s clinical manifestation after comprehensive clinical, biochemical, hematological investigations ruled out other disorders such as parasitosis, Allergic Bronchopulmonary Fungosis, Eosinophilic Granulomatosis with Poly Angitis (EGPA), and Hypereosinophilia syndrome.
Conclusions
Complex patients initially presenting with respiratory conditions in addition to unexplained autoinflammatory conditions are a diagnostic challenge. Genetic molecular testing provides healthcare practitioners with useful information that may diagnose underlying auto-inflammatory diseases in undifferentiated patients. Role of inflammasome-activation in asthma and eosinophilia needs further investigation.
Global emergent responses of stream microbial energetics to glacier shrinkage
Authors: Kohler Tyler , Bourquin Massimo , Peter Hannes , Sinsabaugh Robert L. , Yvon-Durocher Gabriel , Battin Tom 
Published: June 2023

Abstract
Mountain and polar glaciers are melting worldwide. However, the downstream impacts of this unprecedented environmental change on elemental fluxes and microbial energetics in the glacier-fed streams (GFS) remains poorly understood at a global scale. This contrasts the relevance of GFSs in initiating the flow of some of the world’s largest river networks and providing clean water to large human populations. Here, by studying resource stoichiometry in 154 GFSs from Earth’s major mountain ranges, we show that these ecosystems are potentially carbon (C) and phosphorus (P) limited, where P limitation may become exacerbated and C limitation alleviated as glaciers shrink. Modeling threshold elemental ratios from extracellular enzymatic activities, we show that the microbial metabolism in GFSs is indeed C and P limited. This is consistent with low microbial carbon use efficiencies (CUE; median: 0.15) indicative of maintenance metabolism. Using space-for-time substitution approaches, we found increasing biomass of benthic primary producers potentially relieving the microbial metabolism from resource limitation as glaciers shrink. Furthermore, increasing streamwater temperature will stimulate microbial growth (temperature sensitivity: 0.62 electronvolts) in GFSs. Our study unveils responses of the microbial energetics to a greening and warming GFS environment, shifting ecosystem metabolism to autotrophy with consequences for related ecosystem C cycling.
Obstructed Thoracic Duct Stent in a Patient With Recurrence of Chyluria After Treatment
Authors: Hoa Tran Quoc , Cuong Nguyen Ngoc , Hoan Le , Hoang Nguyen , Long Hoang , Hoan Nguyen Cong , Luu Doan Tien 
Published: June 2023

Abstract
Background
Thoracic duct (TD) stenting is considered a possible treatment for some pathological conditions caused by TD obstruction, including chyluria. Several studies reported on the efficacy of TD stent treatment, but few studies have evaluated flow through the stent after being deployed. This report aims to describe the flow through a TD stent and the efficacy of renal-lymphatic fistula embolization in the treatment of chyluria.
Case presentation
we report a case of chyluria treated by TD stent previously, but the symptoms recurred after four months. The second intervention we found the stent was obstructed by debris. We recanalized the stent and successful catheterised the microcatheter through the stent retrograde into the TD then into the renal-lymphatic fistula branch. After embolization of that abnormal branch, no recurrent symptom of chyluria happened.
Conclusion
stent in the TD is foreign body which may be stuck by debris in the TD. The key to treatment of spontaneous chyluria might be the embolization of renal-lymphatic fistula.
Perceptions of indigenous Baganda men regarding the use of LARCs by their rural women
Authors: Kibonire Ronald Arineitwe , Mphuthi DAVID Ditaba 
Published: June 2023

Abstract
Many women's complications during pregnancy, labour, and postpartum are the leading causes of death worldwide. Contraceptives, particularly long-acting reversible contraceptives (LARCs), are the most important and effective interventions for reducing maternal mortality. LARCs assist the mother in delaying pregnancy and permit extended intervals between births. However, the utilisation of LARCs remains low globally and in Uganda, partly due to inadequate male partner support. This study aimed to ascertain the perceptions of rural indigenous Ugandan men regarding the use of LARCs by rural women. The research was conducted in the Ugandan districts of Kiboga. Using purposeful sampling, ten married men aged 20 to 49 were selected for individual interviews. For face-to-face interviews, the researcher used semi-structured questions. Data analysis was performed by transcribing the interviews, arranging the field notes, organising and preserving the data, listening to recordings, perusing field notes and interviews, and then coding and categorising the data to identify the phenomenon's emergent themes. The study found that indigenous Baganda men had perceptions regarding their rural women's use of LARCs, and these perceptions acted as barriers to use. These included that adverse effects, fears, and desires. The study suggests strengthening social and behavioural change in communication, service delivery for LARCs, and monitoring and evaluation systems for LARCs. In addition, policymakers should foster a conducive environment for the provision of LARC services, and should prepare pre-service and in-service healthcare professionals to provide LARC services through health training institutions and universities.