bluuebunny/crossref_metadata_2025_split huggingface dataset:
Evaluation of Acute Myeloid Leukemia Approach in Damascus (Al-Mujtahid) Hospital
Authors: Sulaiman Seham , Hamdan Ahmed Al - , Ali Mohammad AL - 
Published: May 2023

Abstract

Background AML can be diagnosed based on a sample of peripheral blood or bone marrow. In this article we will discuss the role of bone marrow assessment and peripheral blood monitoring in the diagnosis, management, and follow-up of patients with acute myelogenous leukemia (AML). For patients with circulating blast, it is reasonable to conduct the necessary studies for diagnosis and risk classification, including multifactorial flow cytometry, genetics Cellular, and molecular analysis on a peripheral blood sample. A 'pure day 14' assay is used to document cell deficiency in response to chemotherapy, but it is unclear whether this assessment is necessary as it often does not influence immediate management. Current recovery response assessments to assess remission and measure residual disease are based on bone marrow biopsy. Peripheral blood assessment may be sufficient to monitor relapse, but the sensitivity of the bone marrow test in some cases is higher. While bone marrow assessment can certainly be avoided in certain cases, this cumbersome and uncomfortable procedure currently remains the de facto standard for assessing response.Methods A retrospective study targeting 50 patients attending Al-Mujtahid Hospital in Damascus according to certain acceptance and exclusion criteria.Results Our study explored many aspects and factors related to leukemia, as it included two samples of 50 people, the largest proportion of them were males, and the largest proportion of the participants were between the ages of 65 − 20, and also the majority of the participants were non-smokers and non-alcoholic, moving to talk about antecedents, there was a percentage Few suffer from diabetes and arterial hypertension, while not all of the participants suffer from other tumors, as our knowledge has proven a relationship between age and remission, between smoking and AFP, and between insensitivity and complete inactivity.Conclusion We recommend conducting periodic analyzes and not neglecting any general symptom of lack of weight, appetite or heat, as it was the most common among patients. We also recommend the need to stay away from smoking and alcohol. For patients, we recommend the necessity of conducting periodic analyzes in order to monitor complete and partial complacency. We also note the need to monitor minimal residual disease. The remainder because of its importance in the recurrence of the disease
Ultrahigh-modulation-efficiency graphene-silicon micro-ring modulator
Authors: Kong Deming , Liu Yong , Ding Yunhong , Hu Hao , Luan Chao 
Published: May 2023

Abstract
Electro-optic (E/O) modulators are crucial for optical communication but face a trade-off between modulation bandwidth and efficiency. A small footprint could reduce the capacitance and increase the bandwidth. However, this usually results in low modulation efficiency. We address this trade-off by embedding a partially overlapped double-layer graphene on a silicon slot waveguide into an integrated micro-ring modulator. The modulator achieves a modulation bandwidth exceeding 40 GHz with an ultrahigh modulation efficiency of 10.99 V-1µm-1, which is an order of magnitude higher than state-of-the-art E/O modulators. We also demonstrate high reproducibility of the graphene modulator. The compact, highly efficient, and highly reproducible graphene E/O modulator has the potential to enable large-scale graphene photonic integrated circuits, facilitating a broad range of applications such as optical interconnects, optical neural networks, and programmable photonic circuits.
Sequence Analysis and Biochemical Characteristics of Two Non-specific
Lipid Transfer Proteins from Tartary Buckwheat Seeds
Authors: Cui Xiaodong , Li Chen , Tian Wenhua , Wang Wenhua , Li Jiao , Wang Zhuanhua 
Published: May 2023

Introduction:
Plant non-specific lipid transfer proteins (nsLTPs) play an important role in
plant resistance to various stresses, and show potential applications in agriculture, industrial
manufacturing, and medicine. In addition, as more and more nsLTPs are identified as allergens,
nsLTPs have attracted interest due to their allergenicity. Two nsLTPs from Tartary buckwheat have
been isolated and identified. There is a need to study their biochemical characteristics and
allergenicity.

Objective:
The study aims to investigate the biochemical characteristics of two nsLTPs from Tartary
buckwheat seeds and evaluate their potential allergenicity.

Methods:
Two nsLTPs derived from Tartary buckwheat, namely FtLTP1a and FtLTP1b, were
produced by gene cloning, expression, and purification. Sequence analysis and biochemical
characteristics of the proteins, including lipid binding ability, α-amylase inhibition activity, antifungal
activity, and allergenic activity, were investigated.

Results:
High-purity recombinant FtLTP1a and FtLTP1b were obtained. FtLTP1a and FtLTP1b
exhibited similar lipid binding and antifungal properties. Only FtLTP1b showed weak inhibitory
activity against α-amylase.

Conclusion:
FtLTP1b could specifically bind IgE in the serum allergic to buckwheat and cross-react
with pollen (w6). FtLTP1b is a novel allergenic member of the lipid-transfer protein 1 family found in
Tartary buckwheat.
A Stable Cell Line Co-expressing hTRPV1 and GCaMP6s: A Novel
Cell-based Assay For High-throughput Screening of hTRPV1 Agonists
Authors: Shi Jingjing , Chen Xuejun , Zhang Yi , Shi Tong , Zhang Ruihua , Zhu Siqing , Zong Xingxing , Wang Chen , Li Liqin 
Published: May 2023

Background:
Transient receptor potential vanilloid-1 (TRPV1) is a non-selective cation
channel capable of integrating various noxious chemical and physical stimuli. Recently, human
TRPV1 (hTRPV1) has attracted wide attention from researchers because it is closely related to
pain, inflammation, temperature perception, and tumors. Our study was aimed at generating a stable
cell line co-expressing hTRPV1 receptor and GCaMP6s calcium indicator protein and, based
on this, developing high-throughput screening methods for targeting hTRPV1 agonists.

Methods:
The CHO-hTRPV1-GCaMP6s cell line stably expressing hTRPV1 and GCaMP6s was
generated by co-transfection of hTRPV1 and GCaMP6s into Chinese hamster ovary (CHO) cells.
The high-throughput screening methods were developed based on detecting the concentration of
intracellular calcium ions ([Ca2+]i) by using chemically synthesized dyes and genetically encoded
calcium indicator (GECI). Meanwhile, the sensitivity and adaptability of these methods in the
evaluation of capsaicinoids were also compared.

Results:
A stable cell line co-expressing hTRPV1 and GCaMP6s was generated and used to establish
a functional high-throughput screening assay based on the measurement of [Ca2+]i by fluorometric
imaging plate reader (FLIPR). The GECI exhibited a higher sensitivity and applicability
than that of chemically synthesized dyes in detecting the changes in [Ca2+]i induced by capsaicin.
The CHO-hTRPV1-GCaMP6s cell line was further used to detect the dose-dependent relationships
of various hTRPV1 agonists (comparison of EC50 values: capsaicin (39 ± 1.67 nM) < nonivamide
(67 ± 3.05 nM) < piperine (9222 ± 1851 nM)), and this order is consistent with the pharmacological
properties of hTRPV1 activation by these agonists.

Conclusion:
The successful establishment of the CHO-hTRPV1-GCaMP6s cell lines and their
application in high-throughput screening of hTRPV1 agonists.
The Expression and Prognostic Value of Co-stimulatory Molecules in
Clear Cell Renal Cell Carcinoma (CcRcc)
Authors: Wu Chengjiang , Cai Xiaojie , He Chunyan 
Published: May 2023

Background:
Renal cell carcinoma (RCC) was one of the most common malignant cancers in the urinary system. Clear cell carcinoma (ccRCC) is the most common pathological type, accounting for approximately 80% of RCC. The lack of accurate and effective prognosis prediction methods has been a weak link in ccRCC treatment. Co-stimulatory molecules played the main role in increasing anti-tumor immune response, which determined the prognosis of patients. Therefore, the main objective of the present study was to explore the prognostic value of Co-stimulatory molecules genes in ccRCC patients.

Methods:
The TCGA database was used to get gene expression and clinical characteristics of patients with ccRCC. A total of 60 Co-stimulatory molecule genes were also obtained from TCGA-ccRCC, including 13 genes of the B7/ CD28 Co-stimulatory molecules family and 47 genes of the TNF family. In the TCGA cohort, the least absolute shrinkage and selection operator (LASSO) Cox regression model was used to generate a multigene signature. R and Perl programming languages were used for data processing and drawing. Real-time PCR was used to verify the expression of differentially expressed genes.

Results:
The study's initial dataset included 539 ccRCC samples and 72 normal samples. The 13 samples have been eliminated. According to FDR<0.05, there were differences in the expression of 55 Co-stimulatory molecule genes in ccRCC and normal tissues. LASSO Cox regression analysis results indicated that 13 risk genes were optimally used to construct a prognostic model of ccRCC. The patients were divided into a high-risk group and a low-risk group. Those in the high-risk group had significantly lower OS (Overall Survival rate) than patients in the low-risk group. Receiver operating characteristic (ROC) curve analysis confirmed the predictive value of the prognosis model of ccRCC (AUC>0.7). There are substantial differences in immune cell infiltration between high and low-risk groups. Functional analysis revealed that immune-related pathways were enriched, and immune status was different between the two risk groups. Real-time PCR results for genes were consistent with TCGA DEGs.

Conclusion:
By stratifying patients with all independent risk factors, the prognostic score model developed in this study may improve the accuracy of prognosis prediction for patients with ccRCC.
Study of work of PVC membranes for engineering waterproofing under increased seismic conditions
Authors: Shalimov V. N. , Tsybenko A. V. , Goglev I. N. , Loginova S. A. 
Published: May 2023

Objective. At present, increased requirements are imposed on waterproofing materials used in seismic hazard zones. The aim of the study is to assess the possibility of using LOGICBASE™ polymer membranes in regions of increased seismic activity in the Russian Federation.Method. The mechanism of operation of polymeric membranes in foundation structures under conditions of constant displacement and friction is considered. Experiments were carried out to determine the coefficient of friction according to the method of state standards on a tensile testing machine MIRK-1000K. Samples of a round shape made of polymeric membranes were studied on a special multiaxial stretching device in accordance with the requirements of state standards.Result. Multiaxial tensile testing of LOGICBASE™ V-SL polymeric membranes revealed that the maximum tensile strength of the samples was 6948.22 kPa (~ 6.95 MPa), and the elongation of the samples at break was 113.89%. The coefficient of friction in the system "polymer waterproofing material - concrete structure" was determined under conditions of increased seismic activity according to the MSK-64 scale.Conclusion. According to the studies, it was concluded that PVC membranes for engineering waterproofing can be used in construction areas with seismicity up to 9 points inclusive on the MSK-64 scale.
Facilitators and Barriers to the Sustainability of eHealth Solutions in Low- and Middle-Income Countries: Descriptive Exploratory Study
Authors: Mamuye Adane , Nigatu Araya Mesfin , Chanyalew Moges Asressie , Amor Lamia Ben , Loukil Sihem , Moyo Chris , Quarshie Samuel , Antypas Konstantinos , Tilahun Binyam 
Published: May 2023

Background
Despite the widely anticipated benefits of eHealth technologies in enhancing health care service delivery, the sustainable usage of eHealth in transitional countries remains low. There is limited evidence supporting the low sustainable adoption of eHealth in low- and middle-income countries.

Objective
The aim of this study was to explore the facilitators and barriers to the sustainable use of eHealth solutions in low- and middle-income nations.

Methods
A qualitative descriptive exploratory study was conducted in 4 African nations from September to December 2021. A semistructured interview guide was used to collect the data. Data were audio-recorded and transcribed from the local to the English language verbatim, and the audio data were transcribed. On the basis of the information gathered, we assigned codes to the data, searched for conceptual patterns, and created emerging themes. Data were analyzed thematically using OpenCode software.

Results
A total of 49 key informant interviews (10 from Tunisia, 15 from Ethiopia, 13 from Ghana, and 11 from Malawi) were conducted. About 40.8% (20/49) of the study participants were between the ages of 26 and 35 years; 73.5% (36/49) of them were male participants; and 71.4% (35/49) of them had a master’s degree or higher in their educational background. Additionally, the study participants' work experience ranged from 2 to 35 years. Based on the data we gathered, we identified 5 themes: organizational, technology and technological infrastructure, human factors, economy or funding, and policy and regulations.

Conclusions
This study explores potential facilitators and barriers to long-term eHealth solution implementation. Addressing barriers early in the implementation process can aid in the development of eHealth solutions that will better fulfill the demands of end users. Therefore, focusing on potential challenges would enhance the sustainability of eHealth solutions in low- and middle-income countries.
Recovery of Breakthrough Asthma Attacks Treated With Oral Steroids While on Monoclonal Antibody Therapy: Protocol for a Prospective Observational Study (BOOST)
Authors: Howell Imran , Mahdi Mahdi , Bafadhel Mona , Hinks Timothy S C , Ramakrishnan Sanjay , Melhorn James , Jabeen Maisha , Pavord Ian D 
Published: May 2023

Background
Asthma attacks are a common and important problem. Someone experiences an asthma attack in the United Kingdom every 10 seconds. Asthma attacks cause coughing, wheezing, breathlessness, and chest tightness and are highly stressful for patients. They result in reduced quality of life, with days lost from work or school. Asthma attacks are treated with oral corticosteroids (OCSs), but these have many short- and long-term side effects. Asthma monoclonal antibodies (mAbs) have revolutionized the treatment of severe asthma by reducing asthma attacks and OCS burden by over 50%, but some people still experience attacks while on mAbs. The MEX study showed that residual asthma attacks are broadly eosinophilic (high fractional exhaled nitric oxide [FeNO]) or noneosinophilic (low FeNO), but it did not measure response to OCS treatment. There is an evidence gap in understanding the clinical and inflammatory responses that occur when using OCSs to treat residual asthma attacks in patients taking asthma mAbs.

Objective
The primary objective is to compare the clinical recovery between high-FeNO and low-FeNO attacks after acute treatment with oral prednisolone among people established on long-term asthma mAb treatment. The exploratory objective is to compare the inflammatory response to acute treatment with oral prednisolone between high-FeNO and low-FeNO attacks.

Methods
BOOST (Breakthrough Asthma Attacks Treated With Oral Steroids) is a single-center, prospective observational study of 60 adults established on long-term asthma mAb treatment who receive acute treatment with oral prednisolone (usual care) for an asthma attack. The primary outcome will be the proportion of treatment failure (the need to start oral prednisolone or antibiotics or an unscheduled health care visit for asthma, following an attack) at day 28. The secondary outcomes will be the change in forced expiratory volume in 1 second and the change in visual analogue scale symptom score between the stable state, attack, day 7, and day 28 visits. The exploratory outcomes include the changes in sputum, nasal, and blood inflammometry between the stable state, attack, day 7, and day 28 visits.

Results
The last asthma attack visit is anticipated to occur in December 2023. Data analysis and publication will take place in 2024.

Conclusions
We will test the hypothesis that there is a difference in the rate of recovery of clinical and inflammatory measures between high-FeNO and low-FeNO asthma attacks that occur in patients on mAb therapy. The study data will help power a future randomized placebo-controlled trial of prednisolone treatment for nonsevere attacks in patients treated with asthma mAbs and will provide important information on whether corticosteroid treatment should be FeNO-directed.

International Registered Report Identifier (IRRID)
DERR1-10.2196/46741
Mengkaji Publikasi Dosen Menggunakan Analisis Bibliometrik
Authors: Mubarok Aly , Istiana Purwani 
Published: May 2023

INTISARI
Pertumbuhan publikasi menjadi perhatian sebuah institusi pendidikan tinggi. Capaian jumlah publikasi menjadi salah satu indikator kinerja institusi pendidikan tinggi. Tujuan penelitian ini adalah mendekripsikan profil dokumen dosen Fakultas Farmasi yang terindeks pada database bereputasi Internasional (Scopus). Deskripsi profil dokumen tersebut meliputi, sebaran dokumen berdasarkan tahun, jenis tipe dokumen, penulis/dosen paling produktif, dokumen berdasarkan sumber, dokumen yang paling banyak disitir, dan tren topik riset dosen. Data dikumpulkan berdasarkan Scopus ID dosen. Analisis bibliometrik digunakan pada penelitian ini terhadap 989 dokumen. Aplikasi Vosviewer digunakan untuk memvisualiasikan kata kunci. Hasil penelitian menunjukkan bahwa pada rentang tahun 2012-2021, dokumen terbanyak terjadi pada tahun 2020 yakni mencapai 217 dokumen. Tipe dokumen terbanyak adalah artikel jurnal mencapai 88, 465. Penulis/dosen paling produktif dengan 252 dokumen adalah Rohman, Abdul. Karya dosen paling banyak terdapat pada sumber Journal of applied Pharmaceutical Science. Artikel yang berjudul Paper-Based Microfluidic Devices: Emerging Themes and Applications merupakan artikel yang paling banyak disitir dan berdasarkan hasil visualisasi kata kunci menggunakan aplikasi Vosviewer diketahui kata kunci Ftir Spectroscopy, Indonesia dan Chemometrics merupakan kata kunci yang dominan muncul pada dokumen rentang tahun 2012-2021.
The impacts of school on English learners’ motivation in Indonesian Islamic schools
Authors: Farid Achmad , Adara Reza Anggriyashati , Ashar Salim 
Published: May 2023

Among the teaching practitioners in Indonesia, there is a general belief that some learners of English in some Indonesian pesantren (Islamic boarding schools) have a negative view on English, which consequently affects their motivation. However, it remains unclear what has affected the trend. The current study investigates this issue in three pesantren schools in Indonesia affiliated with different Islamic groups. Using the L2 Motivational Self System (L2MSS) model proposed by Dörnyei (2009) as an analytical lens, a survey instrument was designed to describe and compare students’ motivation to learn English (n = 376). A multivariate analysis of variance (MANOVA) was run to describe and compare the students’ motivation, and a stepwise multiple regression analysis was performed to discover which motivational factors made the most significant contribution to motivation by predicting reported learning effort in English. In addition, a thorough document analysis was performed to obtain background information regarding the teaching of English in the three institutions. It was found that institutional factors do play a role in influencing the learners’ motivation, in that in the most religiously conservative context, the school policy does shape the learners’ ideal L2 self. As the learners’ ideal L2 self was weak, it leads to weak motivational power as well. The paper concludes by emphasizing the crucial role of educational contexts in shaping students’ ideal selves and in providing favourable learning experience, which are key elements in motivating learners to learn English.
Weighing research competencies of department of English language education graduates at UIN Ar-Raniry Banda Aceh
Authors: Dahliana Syarifah , Akmal Saiful , Phonna Rizka Malda 
Published: May 2023

The purpose of this study is to scrutinize participants’ research competency, source of research competency, and problems related to the research conduct. Nine graduates of the Department of English Language Education were purposefully selected; three of them completed their theses in one semester, three others finished in two semesters, and one other finalized in three semesters. In this qualitative study, semi-structured interviews were used. The findings revealed that graduates with one-semester thesis completion rates have stronger research proficiency than other groups. They have a higher motivational value component, a higher methodology-reflective component, and a higher operational activity component of research competency. Meanwhile, the emotional-volitional component of research competency remains equal for each group of participants. Furthermore, graduates acquired research competency through coursework, research conduct, and self-study. In terms of problems with research conduct, the data disclosed that graduates frequently struggled with self-motivation, supervisors, time/class schedule, knowledge of prior studies, data analysis, and participants selection.
EFEKTIVITAS RUMPUT BEBEK (Lemna minor) DALAM PENYISIHAN KADMIUM (Cd) DAN CHEMICAL OXYGEN DEMAND (COD) PADA LINDI TPA SAMPAH GAMPONG JAWA KOTA BANDA ACEH
Authors: Harahap Juliansyah , Irhamni  , Nazri Adhlani  , T. Muhammad Ashari  
Published: May 2023

Lemna minor plant have the ability to reduce pollutant levels in Landfill leachate. The use of Lemna minor in leachate treatment was considered effective in degrading Cd and COD pollutants. The objective of this study was to determine the effectiveness and absorption rate of Lemna minor in degrading heavy metal Cd and reducing COD levels in Gampong Jawa Landfill leachate. This study used the phytoremediation method on a laboratory scale, and the leachate sampling method used the grab sampling technique. In testing both parameters, two types of test variations were carried out. First, in Reactor I, it was carried out without using an aerator, and in Reactor II, the test was carried out with the addition of an aerator. For duration, the variations of retention time were used are the third, sixth, ninth, and 12th days. The weight of the Lemna minor were used in each reactor was 35 grams for a leachate volume of 5 Liters. The results showed that Lemna minor was able to reduce pollutant levels in the Gampong Jawa Landfill leachate with the most optimal proportion of 15.92% for the heavy metal Cd, and 94.68% for the organic pollutant COD. Treatment on the 12th day for tests carried out in reactors that have an aeration system was the most effective variation in reducing the levels of Cd and COD parameters.
Keywords : Lemna minor, Landfill Leachate, Phytoremediation