bluuebunny/crossref_metadata_2025_split huggingface dataset:
Prognostic significance of cyclin D1 expression pattern in HPV‐negative oral and oropharyngeal carcinoma: A deep‐learning approach
Authors: Yang Ke , Zhu Guixin , Sun Yanan , Hu Yaying , Lv Yinan , Li Yiwei , Pan Juncheng , Chen Fu , Zhou Yi , Zhang Jiali 
Published: September 2023

AbstractBackgroundWe aimed to establish image recognition and survival prediction models using a novel scoring system of cyclin D1 expression pattern in patients with human papillomavirus‐negative oral or oropharyngeal squamous cell carcinoma.MethodsThe clinicopathological data of 610 patients with human papillomavirus‐negative oral/oropharyngeal squamous cell carcinoma were analyzed retrospectively. Cox univariate and multivariate risk regression analyses were performed to compare cyclin D1 expression pattern scoring with the traditional scoring method—cyclin D1 expression level scoring—in relation to patients' overall and progression‐free survival. An image recognition model employing the cyclin D1 expression pattern scoring system was established by YOLOv5 algorithms. From this model, two independent survival prediction models were established using the DeepHit and DeepSurv algorithms.ResultsCyclin D1 had three expression patterns in oral and oropharyngeal squamous cell carcinoma cancer nests. Superior to cyclin D1 expression level scoring, cyclin D1 expression pattern scoring was significantly correlated with the prognosis of patients with oral squamous cell carcinoma (p < 0.0001) and oropharyngeal squamous cell carcinoma (p < 0.05). Moreover, it was an independent prognostic risk factor in both oral squamous cell carcinoma (p < 0.0001) and oropharyngeal squamous cell carcinoma (p < 0.05). The cyclin D1 expression pattern‐derived image recognition model showed an average test set accuracy of 78.48% ± 4.31%. In the overall survival prediction models, the average concordance indices of the test sets established by DeepSurv and DeepHit were 0.71 ± 0.02 and 0.70 ± 0.01, respectively.ConclusionCombined with the image recognition model of the cyclin D1 expression pattern, the survival prediction model had a relatively good prediction effect on the overall survival prognosis of patients with human papillomavirus‐negative oral or oropharyngeal squamous cell carcinoma.
Actions speak louder than words: Home visits and its effect on dietary adherence in patients with phenylketonuria
Authors: Bulut Fatma Derya , Kor Deniz , Kilavuz Sebile , Cicek Ebru , Koseci Burcu , Kara Esra , Burgac Ezgi , Kaplan İrem , Onenli Mungan Neslihan 
Published: September 2023

AimPhenylketonuria (PKU) is an inherited metabolic disorder in which accumulation of phenylalanine (Phe) leads to poor neurological outcomes without treatment. Dietary therapy is the main treatment and nonadherence is associated with elevated blood Phe levels and correspondingly poor neuropsychiatric outcomes. This study aimed to examine the effect of home visits on blood Phe levels in PKU patients.MethodsSixty‐five paediatric PKU patients who were on low‐phenylalanine diet were visited monthly at home for 6 months. At each visit, dietary education was provided, patients' height and weight were measured and blood samples were collected.ResultsTwenty‐eight (43.1%) patients had classic PKU and 37 (56.9%) had moderate PKU. Blood Phe levels decreased statistically significant at first, second, fifth, and sixth months compared with screening visit. Blood Phe levels in moderate PKU patients decreased significantly at the last visit unlike classic PKU patients. A significant decrease in blood Phe levels was observed in patients older than 10 years. Anthropometric parameters improved.ConclusionsDietary nonadherence is the main treatment failure in PKU. Home visits for education are a promising way to improve treatment outcomes by providing quality education, better assessment, and correction of mistakes but they should be ongoing and supported by different interventions that address patients' special needs.
Morphological and pathological characterization of Colletotrichum species causing anthracnose of litchi leaves in Guangxi, China
Authors: Huang Rong , Sun Wenxiu , Guo Tangxun , Huang Suiping , Tang Lihua , Chen Xiaolin , Li Qili 
Published: September 2023

AbstractLitchi (Litchi chinensis Sonn.) is an evergreen subtropical fruit tree native to southern China. Litchi is vulnerable to a wide range of diseases affecting yield and fruit quality. Anthracnose is one of the main diseases during the period of growth and storage, which has a serious impact on the quality and production of litchi. In December 2020 to May 2021, typical anthracnose symptoms were observed on litchi leaves in different orchards in Qinzhou City, Guangxi Province, Southern China. According to colony features, conidial and appressorial morphology, and sequence analysis of several genomic regions (internal transcribed spacer (ITS) region, chitin synthase (chs‐1), actin (act), calmodulin (cal), glyceraldehyde‐3‐phosphate dehydrogenase (gapdh), β‐tubulin (tub2) and the intergenic region of apn2 and MAT1‐2‐1 (ApMat)), 44 isolates were obtained, and 26 were identified as three Colletotrichum species: C. fructicola (50%), C. siamense (42.31%), C. gigasporum (7.69%). The pathogenicity tests were performed with conidial suspension and mycelia plugs to inoculate wounded litchi seedlings. The results of pathogenicity tests showed that the virulence of C. gigasporum was the weakest, and the virulence of C. fructicola was the strongest. This is the first report of C. gigasporum causing anthracnose of litchi worldwide.
Whole genome analyses reveal weak signatures of population structure and environmentally associated local adaptation in an important North American pollinator, the bumble bee Bombus vosnesenskii
Authors: Heraghty Sam D. , Jackson Jason M. , Lozier Jeffrey D. 
Published: September 2023

AbstractStudies of species that experience environmental heterogeneity across their distributions have become an important tool for understanding mechanisms of adaptation and predicting responses to climate change. We examine population structure, demographic history and environmentally associated genomic variation in Bombus vosnesenskii, a common bumble bee in the western USA, using whole genome resequencing of populations distributed across a broad range of latitudes and elevations. We find that B. vosnesenskii exhibits minimal population structure and weak isolation by distance, confirming results from previous studies using other molecular marker types. Similarly, demographic analyses with Sequentially Markovian Coalescent models suggest that minimal population structure may have persisted since the last interglacial period, with genomes from different parts of the species range showing similar historical effective population size trajectories and relatively small fluctuations through time. Redundancy analysis revealed a small amount of genomic variation explained by bioclimatic variables. Environmental association analysis with latent factor mixed modelling (LFMM2) identified few outlier loci that were sparsely distributed throughout the genome and although a few putative signatures of selective sweeps were identified, none encompassed particularly large numbers of loci. Some outlier loci were in genes with known regulatory relationships, suggesting the possibility of weak selection, although compared with other species examined with similar approaches, evidence for extensive local adaptation signatures in the genome was relatively weak. Overall, results indicate B. vosnesenskii is an example of a generalist with a high degree of flexibility in its environmental requirements that may ultimately benefit the species under periods of climate change.
Matrix multipliers, demand composition and income distribution: Post‐Keynesian–Sraffian theory and evidence from the world's ten largest economies
Authors: Mariolis Theodore , Ntemiroglou Nikolaos 
Published: September 2023

AbstractThis paper builds and explores in detail a post‐Keynesian–Sraffian one‐country model of effective demand, which, on the one hand, extends the Kurz multiplier model to open–with state‐economies of single production and, on the other hand, is applicable to data from the National Input–Output Tables of the World Input–Output Database. Thus, it estimates the net output, import and employment matrix demand multipliers for the world's 10 largest economies. The overall findings provide general support for and new insights into Sraffian theoretical and policy arguments: (i) the multiplier effects depend heavily on the physical composition of autonomous demand; (ii) the incremental share of wages in net national income can move in either direction with an increase in the savings ratios out of wages and profits and/or the corresponding direct tax rates; (iii) there is, in general, no one‐to‐one correspondence between alternative tax–transfer policies and their multiplier effects; and (iv) there is not necessarily an inverse relationship between the overall level of the profit rate and the multiplier effects or the wage share.
Illuminating bunyavirus entry into host cells with fluorescence
Authors: Gu Yu , Lozach Pierre‐Yves 
Published: September 2023

AbstractBunyavirales constitute the largest order of enveloped RNA viruses, many members of which cause severe diseases in humans and domestic animals. In recent decades, innovative fluorescence‐based methods have paved the way to visualize and track single fluorescent bunyaviral particles in fixed and live cells. This technological breakthrough has enabled imaging of the early stages of infection and the quantification of every step in the bunyavirus cell entry process. Here, we describe the latest procedures for rendering bunyaviral particles fluorescent and discuss the advantages and disadvantages of each approach in light of the most recent advances in fluorescence detection and monitoring of bunyavirus entry. In this mini‐review, we also illustrate how fluorescent viral particles are a powerful tool for deciphering the cellular entry process of bunyaviruses, the vast majority of which have not yet been analyzed.
Adeno‐associated virus‐9 reverses delayed gastric emptying of solids in diabetic mice
Authors: Li Xiaojie , Ji Sihan , Cipriani Gianluca , Hillestad Matthew L. , Eisenman Seth T. , Barry Michael A. , Nath Karl A. , Linden David R. , Wright Alec , AlAsfoor Shefaa , Grover Madhusudan , Sha Lei , Hsi Linda C. , Farrugia Gianrico 
Published: September 2023

AbstractBackgroundGastroparesis is defined by delayed gastric emptying (GE) without obstruction. Studies suggest targeting heme oxygenase‐1 (HO1) may ameliorate diabetic gastroparesis. Upregulation of HO1 expression via interleukin‐10 (IL‐10) in the gastric muscularis propria is associated with reversal of delayed GE in diabetic NOD mice. IL‐10 activates the M2 cytoprotective phenotype of macrophages and induces expression of HO1 protein. Here, we assess delivery of HO1 by recombinant adeno‐associated viruses (AAVs) in diabetic mice with delayed GE.MethodsC57BL6 diabetic delayed GE mice were injected with 1 × 1012 vg scAAV9‐cre, scAAV9‐GFP, or scAAV9‐HO1 particles. Changes to GE were assessed weekly utilizing our [13C]‐octanoic acid breath test. Stomach tissue was collected to assess the effect of scAAV9 treatment on Kit, NOS1, and HO1 expression.Key ResultsDelayed GE returned to normal within 2 weeks of treatment in 7/12 mice receiving scAAV9‐cre and in 4/5 mice that received the scAAV9‐GFP, whereas mice that received scAAV9‐HO1 did not respond in the same manner and had GE that took significantly longer to return to normal (6/7 mice at 4–6 weeks). Kit, NOS1, and HO1 protein expression in scAAV9‐GFP‐treated mice with normal GE were not significantly different compared with diabetic mice with delayed GE.Conclusions and InferencesInjection of scAAV9 into diabetic C57BL6 mice produced a biological response that resulted in acceleration of GE independently of the cargo delivered by the AAV9 vector. Further research is needed to determine whether use of AAV mediated gene transduction in the gastric muscularis propria is beneficial and warranted.
SECRETS OF THE DRAVA: BRONZE AGE METALWORK IN CONTINENTAL CROATIA
Authors: Kudelić Andreja , Franjić Ana , Vrdoljak Snježana , Radivojević Miljana 
Published: September 2023

SummaryThe article presents a group of Bronze Age artefacts recovered from the Drava river wetlands in continental Croatia, examining their typological and chronological markers, and assessing the technological characteristics of the material through spectrometric and use‐wear analyses. We discuss the context of the finds, types of items retrieved and deposition locations, and how these fit into the patterns of European Bronze Age metal deposition practices. Compositional and metalwork use analyses indicate that most of the items were in use prior to their deposition and display solid metallurgical skill. Chronologically, typologically, compositionally, and conceptually, the items align with the depositional trends seen in the wider region and beyond.
Poet as poem: The intermedial staging of A. E. Housman in Tom Stoppard's The Invention of Love
Authors: Yang Huayu , Wang Bowen 
Published: September 2023

AbstractTom Stoppard's The Invention of Love (1997) offers the audience a dream‐like voyage through the post‐mortem reminiscences of the central character, A. E. Housman. The attempt to resurrect Housman, as the historical figure in real life, is suspended by the intertextual incorporation of Housman's poems, the both fictive and enigmatically private voice of which opens up the illusory closure of biographically accurate dramatic characterisation. By fluidising and activating the subversive emotions contained in the formal patterns of the poetic text, the intermedial stage with the corporeality of its theatrical embodiment becomes a spatialised metaphor for the poetry as an open space that is inviting meaning to be projected onto it. The poetic and the theatrical, thus deterritorialised by their intermedial exchanges, interlace a dreamscape in which the nomadic search for the uncorrupted, unappropriated poet/poem continuum provides a transcendental empiricist reading of the ‘truth’ about Housman.
A systematic review and network meta‐analysis comparing different epidural steroid injection approaches
Authors: Mahmoud Atef Mohamed , Shawky Mohamed Ahmed , Farghaly Omer Sayed , Botros Joseph Makram , Alsaeid Mohamed Awad , Ragab Safaa Gaber 
Published: September 2023

AbstractBackgroundLow back pain (LBP) and lumbosacral radiculopathy are frequent disorders that cause nerve root injury, resulting in a variety of symptoms ranging from loss of sensation to loss of motor function depending on the degree of nerve compression.ObjectivesThe goal of this study was to investigate the effectiveness of various epidural injection procedures in adult LBP patients.Study DesignSystematic review and network meta‐analysis.SettingEgypt.MethodsPubMed, Scopus, Web of Science, Cochrane Database, and Embase were used to conduct an electronic literature search. We included RCTs, cohorts, case controls, patients 30 years old with a clinical presentation of low back pain, and comprehensive data on the effects of the intervention on patients with lumbosacral radicular pain who got epidural steroid injections via various techniques. Only papers written in English were eligible.ResultsOur analysis showed that parasagittal intralaminar (PIL) was the most effective approach in decreasing VAS (0–10) in the short term (< 6 months) (MD = −1.16 [95% CI −2.04, −0.28]). The next significant approach was transforaminal (TF) (MD = −0.37 [95% CI −1.14, −0.32]) in the long term; TF was the most effective approach (MD = −0.56 [95% CI −1, −0.13]). According to VAS (0–100) in the short term (< 6 months), our analysis showed an insignificant difference among the injection approaches and in the long term; TF was the most effective approach (MD = −24.20 [95% CI −43.80, −4.60]) and the next significant approach was PIL (MD = −23.89 [95% CI −45.78, −1.99]).LimitationsThe main limitations are the heterogeneity encountered in some of our analyses in addition to studies assessed as high risk of bias in some domains.ConclusionTF was the most effective steroid injection approach. In decreasing VAS for short‐term PIL and TF were the most significant approaches, but TF was the most effective approach in decreasing VAS for the long term. Also, TF was the most effective approach in decreasing ODI for the long term.
BpGRP1 acts downstream of BpmiR396c/BpGRF3 to confer salt tolerance in Betula platyphylla
Authors: Liu Zhongyuan , Zhang Tengqian , Xu Ruiting , Liu Baichao , Han Yating , Dong Wenfang , Xie Qingjun , Tang Zihao , Lei Xiaojin , Wang Chao , Fu Yujie , Gao Caiqiu 
Published: September 2023

SummaryGlycine‐rich RNA‐binding proteins (GRPs) have been implicated in the responses of plants to environmental stresses, but the function of GRP genes involved in salt stress and the underlying mechanism remain unclear. In this study, we identified BpGRP1 (glycine‐rich RNA‐binding protein), a Betula platyphylla gene that is induced under salt stress. The physiological and molecular responses to salt tolerance were investigated in both BpGRP1‐overexpressing and suppressed conditions. BpGRF3 (growth‐regulating factor 3) was identified as a regulatory factor upstream of BpGRP1. We demonstrated that overexpression of BpGRF3 significantly increased the salt tolerance of birch, whereas the grf3‐1 mutant exhibited the opposite effect. Further analysis revealed that BpGRF3 and its interaction partner, BpSHMT, function upstream of BpGRP1. We demonstrated that BpmiR396c, as an upstream regulator of BpGRF3, could negatively regulate salt tolerance in birch. Furthermore, we uncovered evidence showing that the BpmiR396c/BpGRF3 regulatory module functions in mediating the salt response by regulating the associated physiological pathways. Our results indicate that BpmiR396c regulates the expression of BpGRF3, which plays a role in salt tolerance by targeting BpGRP1.
Assessing the agreement between the pneumatic and the flow‐centrifuge method for estimating xylem safety in temperate diffuse‐porous tree species
Authors: Paligi S. S. , Link R. M. , Isasa E. , Bittencourt P. , Cabral J. S. , Jansen S. , Oliveira R. S. , Pereira L. , Schuldt B. 
Published: September 2023

Abstract
The increasing frequency of global change‐type droughts has created a need for fast, accurate and widely applicable techniques for estimating xylem embolism resistance to improve forecasts of future forest changes.
We used data from 12 diffuse‐porous temperate tree species covering a wide range of xylem safety to compare the pneumatic and flow‐centrifuge method, two rapid methods used for constructing xylem vulnerability curves. We evaluated the agreement between parameters estimated with both methods and the sensitivity of pneumatic measurements to the duration of air discharge (AD) measurements.
There was close agreement between xylem water potentials at 50% air discharged (PAD), estimated with the Pneumatron, and 50% loss of hydraulic conductivity (PLC), estimated with the flow‐centrifuge method (mean signed deviation: 0.12 MPa, Pearson correlation: 0.96 after 15 s of gas extraction). However, the relationship between the estimated slopes was more variable, resulting in lower agreement in the xylem water potential at 12% and 88% PAD/PLC. The agreement between the two methods was not affected by species‐specific vessel length distributions. All pneumatic parameters were sensitive to AD time. Overall agreement was highest at relatively short AD times, with an optimum at 16 s.
Our results highlight the value of the Pneumatron as an easy and reliable tool to estimate 50% embolism thresholds for a wide range of diffuse‐porous temperate angiosperms. Further, our study provides a set of useful metrics for methodological comparisons of vulnerability curves in terms of systematic and random deviations, as well as overall agreement.
Chemodiversity in flowers of Tanacetum vulgare has consequences on a florivorous beetle
Authors: Sasidharan R. , Brokate L. , Eilers E. J. , Müller C. 
Published: September 2023

Abstract
The chemical composition of plant individuals can vary, leading to high intraspecific chemodiversity. Diversity of floral chemistry may impact the responses of flower‐feeding insects.
Tanacetum vulgare plants vary significantly in their leaf terpenoid composition, forming distinct chemotypes. We investigated the composition of terpenoids and nutrients of flower heads and pollen in plants belonging to three chemotypes – dominated either by β‐thujone (BThu), artemisia ketone (Keto) or a mixture of (Z)‐myroxide, santolina triene, and artemisyl acetate (Myrox) – using different analytical platforms. We tested the effects of these differences on preferences, weight gain and performance of adults of the shining flower beetle, Olibrus aeneus.
The terpenoid composition and diversity of flower heads and pollen significantly differed among individuals belonging to the above chemotypes, while total concentrations of pollen terpenoids, sugars, amino acids, and lipids did not differ. Beetles preferred BThu over the Myrox chemotype in both olfactory and contact choice assays, while the Keto chemotype was marginally repellent according to olfactory assays. The beetles gained the least weight within 48 h and their initial mortality was highest when feeding exclusively on floral tissues of the Myrox chemotype. Short‐term weight gain and long‐term performance were highest when feeding on the BThu chemotype.
In conclusion, the beetles showed chemotype‐specific responses towards different T. vulgare chemotypes, which may be attributed to the terpenoid composition in flower heads and pollen rather than to differences in nutrient profiles. Both richness and overall diversity are important factors when determining chemodiversity of individual plants and their consequences on interacting insects.
Prosocial behavior amid violence: The deservingness heuristic and solidarity with victims
Authors: Skigin Natán 
Published: September 2023

AbstractIncidents of state repression and criminal violence trigger disparate public responses: Some cases elicit widespread citizen solidarity with victims while others do not. What explains these different reactions? Public debates surrounding civilian victimization vary in the extent to which they present victims as deserving of help, often engaging in victim‐blaming narratives. I argue that through the use of attributional evidence, individuals primarily determine their level of support for the victims based on whether or not they are deemed deserving of assistance, instead of focusing on alternative information such as their similarity with victims' demographic characteristics or the perpetrator's identity. I test this argument using various forms of evidence, including experimental, observational survey, and qualitative data from Mexico's War on Drugs—one of the most significant contemporary human rights crises that has nonetheless triggered only sporadic solidarity. Consistent with the argument, the results show that narratives characterizing people as responsible for their misfortune reduce prosocial behavior by eroding compassion and perceptions of social norms—whether helping victims is socially acceptable. In contrast, citizens are more likely to aid victims perceived to have little control over their situation. These findings suggest that elite and media discourses crucially shape public responses to violence.