bluuebunny/crossref_metadata_2025_split huggingface dataset:
Controlling the Morphological Features, Aspect Ratio and Emission Patterns of Supramolecular Copolymers by Restricted Dimensional Growth
Authors: Das Gourab , Anand Anjali , Vedhanarayanan Balaraman , Padmakumar Akhil , Praveen Vakayil K. , Ajayaghosh Ayyappanpillai 
Published: July 2023

AbstractOne of the bottlenecks associated with supramolecular polymerization of functional π‐systems is the spontaneous assembly of monomers leading to one‐ or two‐dimensional (1D or 2D) polymers without control over chain length and optical properties. In the case of supramolecular copolymerization of monomers that are structurally too diverse, preferential self‐sorting occurs unless they are closely interacting donor‐acceptor pairs. Herein, it is established that the spontaneous 1D polymerization of a phenyleneethynylene (PE) derivative and the 2D polymerization of a Bodipy derivative (BODIPY) can be controlled by copolymerizing them in different ratios, leading to unusual spindle‐shaped structures with controlled aspect ratio, as evident by scanning electron microscopy (SEM), transmission electron microscopy (TEM) and confocal laser scanning microscopy (CLSM) studies. For example, when the content of BODIPY is 50 % in the BODIPY‐PE mixture, the 1D polymerization of PE is significantly restricted to form elongated spindle‐like structures having an aspect ratio of 4–6. The addition of 75 % of BODIPY to PE resulted in circular spindles having an aspect ratio of 1–2.5, thereby completely restricting the 1D polymerization of PE monomers. Moreover, the resultant supramolecular copolymers exhibited morphology and aspect ratio dependent emission features as observed by the time‐resolved emission studies.
Fully Conjugated Covalent Organic Frameworks: Synthesis, Structures and Applications†
Authors: Xu Yueshuai , Ren Guanshi , Zhang Dianqi , Sun Lishui , Zhao Yingjie 
Published: July 2023

Comprehensive SummaryThis review presents a comprehensive examination of fully conjugated covalent organic frameworks (COFs), which constitute an emerging class of porous materials with immense potential for diverse applications. This article focuses on diversified fully conjugated COFs, including sp2 carbon‐carbon linkages, pyrazine linkages, benzobisoxazole linkages, dioxin linkages, β‐aminoalkenone linkages, etc. The synthesis techniques and structural attributes of these COFs are expounded upon in great detail, along with their potential applications in various fields. The review thus provides a valuable resource for researchers keen on delving into the synthesis and applications of fully conjugated COFs, thereby highlighting their potential for developing novel functional materials with distinctive properties.
Magnesium enhances the graft‐versus‐tumor effect of donor lymphocytic infusion on hematologic malignancies
Authors: Wei Yan , Guo Jiayuan , Lu Ning , Liu Yi , Wang Lijun , Wang Lili , Bo Jian , Li Honghua , Dou Liping , Liu Daihong , Gao Chunji 
Published: July 2023

AbstractDonor lymphocyte infusion (DLI) cures relapsed hematologic malignancies after allogeneic hematopoietic stem cell transplantation through the graft‐versus‐tumor (GVT) effect. Although the important role of magnesium in enhancing immunity has been mentioned in studies, limited clinical data have explored how magnesium affects the efficacy of DLI. Besides, although laboratory data demonstrate that magnesium can enhance CD8+ T cells effector function, whether magnesium regulates the tumor killing effect of peripheral blood mononuclear cells (PBMCs) remains to be explored. Here, for the retrospective study, we collected clinical data of relapsed patients receiving DLI and explored the relationship between different serum magnesium levels and patient outcomes. For in vitro studies, we investigated the effect of magnesium on the cytotoxicity of DLI cells which were PBMCs and preliminarily explored the mechanism. Eighty‐one patients were enrolled in this study. It was found that the high post‐DLI magnesium level was significantly associated with a higher incidence of complete remission (CR) or partial remission (CR/PR) and a higher possibility of survival. The magnesium level after DLI was an independent risk factor of overall survival. In vitro studies proved that increased magnesium enhanced the cytotoxic function of PBMCs on hematologic malignancies. Besides, magnesium modulated LFA‐1 headpiece opening. When blocking the integrin‐ligand interaction between LFA‐1 and ICAM‐1, the regulation effect of magnesium on PBMCs was weakened. Therefore, it was possible that magnesium regulated PBMCs effector function by stimulating LFA‐1. These results show that serum magnesium levels affect immunological responses mediated by donor lymphocytes in hematologic malignancies.
Safety and efficacy of parsaclisib in combination with obinutuzumab and bendamustine in patients with relapsed or refractory follicular lymphoma (CITADEL‐102): A phase 1 study
Authors: Hamadani Mehdi , Coleman Morton , Boccia Ralph , Duras Juraj , Hutchings Martin , Zinzani Pier Luigi , Cordoba Raul , Oreiro Mariana Bastos , Williams Vanessa , Liu Huiqing , Stouffs Michael , Langmuir Peter , Sancho Juan‐Manuel 
Published: July 2023

AbstractParsaclisib is a potent and highly selective PI3Kδ inhibitor that has shown clinical benefit with monotherapy in a phase 2 study in relapsed or refractory (R/R) follicular lymphoma (FL). CITADEL‐102 (NCT03039114), a phase 1, multicenter study, assessed the efficacy of parsaclisib in combination with obinutuzumab and bendamustine in patients with R/R FL. Patients were ≥18 years of age with histologically confirmed and documented CD20‐positive FL, and R/R to previous rituximab‐containing treatment regimens. Part one (safety run‐in) determined the maximum tolerated dose of parsaclisib in combination with standard dosage regimens of obinutuzumab and bendamustine. Part two (dose expansion) was an open‐label, single‐group design evaluating safety, tolerability (primary endpoint), and efficacy (secondary endpoint) of parsaclisib combination therapy. Twenty‐six patients were enrolled in CITADEL‐102 and all patients received parsaclisib 20 mg once daily for 8 weeks, followed by 20 mg once weekly thereafter, in combination with obinutuzumab and bendamustine. One patient in safety run‐in experienced a dose‐limiting toxicity of grade 4 QT interval prolongation that was considered related to parsaclisib. Eight patients (30.8%) discontinued treatment due to treatment‐emergent adverse events (TEAEs) of colitis (2 [7.7%]), alanine aminotransferase and aspartate aminotransferase increase (both in one patient [3.8%]), neutropenia, thrombocytopenia, QT prolongation, tonsil cancer, and maculopapular rash (each 1 [3.8%]). The most common reported TEAEs were pyrexia (53.8%), neutropenia (50.0%), and diarrhea (46.2%). Twenty‐three patients (88.5%) experienced grade 3 or 4 TEAEs; the most common were neutropenia (34.6%), febrile neutropenia (23.1%), and thrombocytopenia (19.2%). Seventeen patients (65.4%) had a complete response and 3 patients (11.5%) had a partial response, for an objective response rate of 76.9%. Overall, results from CITADEL‐102 suggest that the combination of parsaclisib with obinutuzumab and bendamustine did not result in unexpected safety events, with little evidence of synergistic toxicity, and demonstrated preliminary efficacy in patients with R/R FL who progressed following prior rituximab‐containing regimens.
Pyogenic liver abscess complicated with endogenous endophthalmitis caused by Klebsiella pneumoniae: A case report and Literature Review
Authors: Chen Yunjiang , Gong Yanchun , Song Bei , Du Yueling , Cai Kaiyu 
Published: July 2023

AbstractObjectivePyogenic liver abscess (PLA) is a common surgical infectious disease caused by various pathogens. Klebsiella pneumoniae is a relatively recent cause, often affecting patients with low immunity. Endogenous endophthalmitis (EE), a rare and serious complication of PLA, may appear with eye symptoms before PLA. By reviewing a case of Klebsiella pneumoniae‐induced PLA complicated with EE, we want to summarize the information about the characteristics, causes, and complications of PLA based on the literature review.MethodsThis case report describes a 37‐year‐old male who had fever high to 39°C for 10 days experienced blurred vision followed by nonlight perception vision. He reported a history of diabetes irregularly taking oral medications and insulin therapy. Imaging examination found a large low‐density area in the right lobe of the liver with an unclear border and vague surrounding fat gap. The blood culture was not positive. The culture of the drainage fluid from the liver puncture showed Klebsiella pneumonia. Blood and liver puncture drainage fluid were sent for microbial high‐throughput gene detection with next‐generation sequencing technology (NGS), which confirmed the diagnosis of Klebsiella pneumoniae‐induced PLA complicated with EE.ResultsThe patient's surgical incision had healed well at discharge, and he could feel light at his left eye. But the patient was lost to follow‐up since the third month after discharge.ConclusionBy reviewing this case and summarize the information about the characteristics, causes, and complications of PLA based on the literature review, we concluded that it is necessary to promptly perform liver puncture drainage and empirically use antibiotics for patients with PLA, especially those with poor glycemic control, to avoid serious complications such as EE.
Recent Advances in Electrochemical, Ni‐Catalyzed C−C Bond Formation
Authors: Franke Mareena C. , Weix Daniel J. 
Published: July 2023

AbstractNickel‐catalyzed cross‐electrophile coupling (XEC) is an efficient method to form carbon‐carbon bonds and has become an important tool for building complex molecules. While XEC has most often used stoichiometric metal reductants, these transformations can also be driven electrochemically. Electrochemical XEC (eXEC) is attractive because it can increase the greenness of XEC and this potential has resulted in numerous advances in recent years. The focus of this review is on electrochemical, Ni‐catalyzed carbon‐carbon bond forming reactions reported since 2010 and is categorized by the type of anodic half reaction: sacrificial anode, sacrificial reductant, and convergent paired electrolysis. The key developments are highlighted and the need for more scalable options is discussed.
How to treat jatrogenic ureteral injury after posterior spinal surgery? Case report and review of literature
Authors: Kavaric Petar , Magdelinic Aleksandar , Rebronja Almir , Albijanic Marko , Sabovic Eldin , Radovic Nenad , Vukovic Marko 
Published: July 2023

IntroductionEntry into the retroperitoneal space during open posterior spinal surgery introduces the rare possibility of iatrogenic ureteral injury.Case presentationWe describe a case of ureteral injury after spinal surgery in a 49‐year‐old female with persistent lumbar pain and high fever 2 weeks after spinal surgery. After admission to the urology department, a computer tomography scan was performed and revealed right‐side hydronephrosis grade III and large retroperitoneal fluid collection. After radiological confirmation of right ureteral injury, a ureteral stent was placed, but 4 weeks later, ureteral stricture was confirmed on antegrade pyelography. Therefore, surgical ureteroplasty was indicated 2 months after initial admission to the urology department. Six weeks later, the stent was removed, and intravenous pyelography revealed a normal ureteral passage.ConclusionThere should be a low threshold for ureteral injuries after spinal cord surgery in patients with high fever and elevated blood creatinine levels.
StainGAN: Learning a structural preserving translation for white blood cell images
Authors: Huang Maoye , Wang Tao , Cai Yuanzheng , Fan Haoyi , Li Zuoyong 
Published: July 2023

AbstractAnalysis of white blood cells in blood smear images plays a vital role in computer‐aided diagnosis for the analysis and treatment of many diseases. However, different techniques for blood smear preparation result in images with large appearance variations, which limits the performance of large‐scale machine learning algorithms. In this paper, we propose StainGAN, an image translation framework to transform the conventional Wright‐stained white blood cell images into their rapidly‐stained counterpart. Moreover, we designed a cluster‐based learning strategy that does not require manual annotations and a multi‐scale discriminator that incorporates a richer hierarchy of the spatial context to generate sharper images with better semantic consistency. Experimental results on multiple real‐world datasets prove the effectiveness of our proposed framework. Moreover, we show that the transformed images from StainGAN can be used to boost the downstream segmentation performance under the label‐limiting scenario.
Interpersonal skills and STEM career choice of three types of FIRST mentors
Authors: Rocker Yoel Shahaf , Dori Yehudit Judy 
Published: July 2023

AbstractBackgroundFor Inspiration and Recognition of Science and Technology (FIRST) robotics is an international, extra‐curricular program that fosters young students' interpersonal skills and career choices in science, technology, engineering, and mathematics (STEM). FIRST teams are guided by mentors, about half of whom are also mentees.PurposeTo describe and characterize FIRST mentors and their perceptions of their own interpersonal skills and STEM career choice and identify differences by mentor types and gender.MethodThe study participants included 261 FIRST mentors. A convergent mixed‐methods approach was used. Data was collected quantitatively via questionnaires and qualitatively via interviews. The analysis was guided by the social cognitive career theory (SCCT).ResultsFourteen categories were identified to describe and characterize the FIRST mentors. Nine were based on SCCT and five were new: influence of friends, interpersonal skill, personal contribution, challenges, and mentor‐as‐educator. Differences were found between three types of FIRST mentors: non‐FIRST mentors, graduate mentors, and mentee mentors. The correlations between factors and categories indicated that the mentors' perceptions were consistent with those of the mentees. The FIRST program impacted the STEM career choice of men more than women, and external motivation influenced women more than men.ConclusionsThe FIRST program contributes to developing its graduates' interpersonal skills and affects their STEM career choice. The study provides insights into mentors' influence on mentees' career choices, from which both genders benefit. Making FIRST available in schools as a widespread enrichment program is expected to foster students' STEM career choices, thereby contributing to the human resource reservoir of the high‐tech industry workforce.
Therapeutic efficacy of the resorcylic acid lactone LL‐Z1640‐2 for adult T‐cell leukaemia/lymphoma
Authors: Oura Masahiro , Harada Takeshi , Oda Asuka , Teramachi Jumpei , Nakayama Atsushi , Sumitani Ryohei , Inoue Yusuke , Maeda Yusaku , Sogabe Kimiko , Maruhashi Tomoko , Takahashi Mamiko , Fujii Shiro , Nakamura Shingen , Miki Hirokazu , Nakamura Masafumi , Hara Tomoyo , Yamagami Hiroki , Kurahashi Kiyoe , Endo Itsuro , Hasegawa Hiroo , Fujiwara Hiroshi , Abe Masahiro 
Published: July 2023

AbstractAdult T‐cell leukaemia/lymphoma (ATL) remains incurable. The NF‐κB and interferon regulatory factor 4 (IRF4) signalling pathways are among the critical survival pathways for the progression of ATL. TGF‐β‐activated kinase 1 (TAK1), an IκB kinase‐activating kinase, triggers the activation of NF‐κB. The resorcylic acid lactone LL‐Z1640‐2 is a potent irreversible inhibitor of TAK1/extracellular signal‐regulated kinase 2 (ERK2). We herein examined the therapeutic efficacy of LL‐Z1640‐2 against ATL. LL‐Z1640‐2 effectively suppressed the in vivo growth of ATL cells. It induced in vitro apoptosis and inhibited the nuclear translocation of p65/RelA in ATL cells. The knockdown of IRF4 strongly induced ATL cell death while downregulating MYC. LL‐Z1640‐2 as well as the NF‐κB inhibitor BAY11‐7082 decreased the expression of IRF4 and MYC at the protein and mRNA levels, indicating the suppression of the NF‐κB‐IRF4‐MYC axis. The treatment with LL‐Z1640‐2 also mitigated the phosphorylation of p38 MAPK along with the expression of CC chemokine receptor 4. Furthermore, the inhibition of STAT3/5 potentiated the cytotoxic activity of LL‐Z1640‐2 against IL‐2‐responsive ATL cells in the presence of IL‐2. Therefore, LL‐Z1640‐2 appears to be an effective treatment for ATL. Further studies are needed to develop more potent compounds that retain the active motifs of LL‐Z1640‐2.
Discovering the effect of co‐fermentation involving Saccharomyces cerevisiae and Schizosaccharomyces pombe on the sensory quality improvement of mandarin wine based on metabolites and transcriptomic profiles
Authors: Luo Xiaoqin , Li Yumeng , Zhong Kai , Luo Dong , Wu Yanping , Gao Hong 
Published: July 2023

AbstractBACKGROUNDMandarin wine has high added value, which can extend the industry chain of mandarins with excellent economic results. However, innovative fermentation methods are urgently needed to improve the typical taste and flavor characteristics of mandarin wine. In this study, the effect and underlying mechanism of co‐fermentation with Saccharomyces cerevisiae and Schizosaccharomyces pombe on the characteristics of mandarin wine were investigated based on integrated metabolomic and transcriptomic analyses.RESULTSIn comparison with fermentation with only S. cerevisiae, the mandarin wine produced from co‐fermentation with S. cerevisiae and Sc. pombe had a higher pH value, lower malic acid content, and more abundant free amino acids, resulting in better sensory evaluation scores. The introduction of Sc. pombe extended the stage of alcoholic fermentation and enhanced the richness and diversity of volatile compounds, especially floral and fruity aroma compounds, including ethyl hexanoate, ethyl caprylate, ethyl enanthate, 1‐heptanol, and phenylethyl alcohol. he significantly differential metabolites and varying genes were mainly found in pathways of glycolysis, pyruvate metabolism, the citrate cycle, and amino acid metabolism.CONCLUSIONCo‐fermentation with S. cerevisiae and Sc. pombe showed advantages in producing distinctive taste and flavor of mandarin wine in comparison with fermentation with only S. cerevisiae. This study can inspire new co‐fermentation strategies to improve the sensory quality of mandarin wine. © 2023 Society of Chemical Industry.
No Correlation between Plasma GPNMB Levels and Multiple System Atrophy in Chinese Cohorts
Authors: Wan Linlin , Fu You , Chen Zhao , Long Zhe , Chen Daji , Yuan Xinrong , Zhu Sudan , Peng Linliu , Liu Wuping , Qiu Rong , Tang Beisha , Jiang Hong 
Published: July 2023

AbstractBackgroundGlycoprotein nonmetastatic melanoma protein B (GPNMB) has been demonstrated to mediate pathogenicity in Parkinson's disease (PD) through interactions with α‐synuclein, and plasma GPNMB tended to be a novel biomarker for PD.ObjectiveThe goal of this study was to investigate whether plasma GPNMB could act as a potential biomarker for the clinical diagnosis and severity monitoring of multiple system atrophy (MSA), another typical synucleinopathy.MethodsPlasma GPNMB levels in patients with MSA, patients with PD, and healthy control subjects (HCs) were quantified using enzyme‐linked immunosorbent assays.ResultsA total of 204 patients with MSA, 65 patients with PD, and 207 HCs were enrolled. The plasma GPNMB levels in patients with MSA were similar to those in HCs (P = 0.251) but were significantly lower than those in patients with PD (P = 0.003). Moreover, there was no significant correlation detected between the plasma GPNMB levels and disease severity scores of patients with MSA.ConclusionsNo evidence was detected for the biomarker potential of plasma GPNMB in MSA. © 2023 International Parkinson and Movement Disorder Society.
Opitz GBBB syndrome with total anomalous pulmonary venous connection: A new MID1 gene variant
Authors: Perea‐Cabrera Maryangel , Granados‐Riveron Javier T. , Segura‐Stanford Begoña , Moreno‐Vargas Liliana M. , Prada‐Gracia Diego , Moran‐Espinosa Mari C. , Erdmenger Julio , Diaz‐Garcia Hector , Sánchez‐Urbina Rocío 
Published: July 2023

AbstractBackgroundOpitz GBBB syndrome (GBBB) is an X‐linked disease characterized by midline defects, including congenital heart defects. We present our diagnostic approach to the identification of GBBB in a consanguineous family in which two males siblings were concordant for a total anomalous connection of pulmonary veins and minor facial dysmorphias.MethodsTargeted exome sequencing analysis of a 380‐gene panel associated with cardiovascular disease was performed on the propositus. Interpretative analysis of the exome results was conducted, and 3D models of the protein changes were generated.ResultsWe identified a NM_000381.4:c.608G>A;p.(Arg203Gln) change in MID1, affecting the conformation of the B‐box 2 domain of the protein, with a zinc finger structure and associated protein interactions. This clinical phenotype is consistent with GBBB; however, the type of congenital heart disease observed in this case has not been previously reported.ConclusionA new likely pathogenic variant on MID1 c.608G>A was found to be associated with Opitz GBBB syndrome.
Unlocking the genomes of formalin-fixed freshwater fish specimens: An assessment of factors influencing DNA extraction quantity and quality
Authors: Brino Erin E. , Schumann David A. , Rezac Calvin R. , Ellwanger Robbie J. , Osmundson Todd W. 
Published: July 2023

Abstract

Objective
Recent technological developments may facilitate the description of evolutionary relationships and population genetic structure as well as other information relevant to fisheries management by using readily available natural history collections. Contemporary sequence capture and short-read sequencing methods offer opportunities to analyze highly fragmented DNA from formalin-fixed specimens so long as enough DNA of sufficient quality is recovered.

Methods
We compared two protocols developed to extract DNA from formalin-fixed tissues using specimens of three freshwater fishes: the Southern Brook Lamprey Ichthyomyzon gagei, Slimy Sculpin Cottus cognatus, and Brown Trout Salmo trutta. Extractions were attempted using hot alkali digestion with and without buffer wash pretreatments to compare the DNA concentration, purity, and fragment length of DNA recovered between extraction protocols, tissue types (muscle and caudal fin tissue for Brown Trout and Slimy Sculpin), and preservation periods (5 or 7 years for Southern Brook Lamprey).

Result
Likelihood models generally did not detect DNA quantity differences between extraction protocols nor tissue types; however, 6.0–8.7× more DNA was recovered from Slimy Sculpin caudal fins than from muscle tissue. Extraction protocol had mixed effects on DNA purity; the wash protocol outperformed the no-wash protocol for Slimy Sculpin and Brown Trout, but the reverse was true for the lamprey. Purer DNA was recovered from the caudal fins; however, fragment lengths were generally greater from muscle tissue for both ray-finned species.

Conclusion
Our results suggest that the best tissue for sampling may depend on the quality metric considered most important for a study's objectives and that omitting time-consuming tissue wash steps can yield DNA of quantity and quality comparable to DNA from more complex methods. Regardless of species, the DNA extracted from most samples using both protocols met quantity and quality thresholds that are likely to result in short-read sequencing success. These results provide optimism for unlocking the wealth of genetic information in natural history collections for use in fisheries management and conservation genomics.