bluuebunny/crossref_metadata_2025_split huggingface dataset:
Accelerated biological aging, inflammatory bowel disease, genetic susceptibility and life expectancy: Evidence from UK Biobank and All of Us Cohorts
Authors: Chen Hao , Li Lingyi , Zhang Han , Zhang Lijun , Long Yu , Feng Jing , Ma Yuying , Wu Yanjun , Meng Meijun , Zeng Ruijie , Luo Dongling , Wang Yi , Leung Felix , Duan Chongyang , Sha Weihong 
Published: January 2025

Abstract

Background: Inflammatory bowel disease (IBD) is a chronic condition affecting individuals across all age groups. However, the association between IBD and biological aging remains unclear.
Methods: We utilized data from the UK Biobank and the diverse cohort of the All of Us (AoU) Research Programme to investigate the role of biological aging in the development of IBD and its subtypes. Biological age was assessed using the Klemera-Doubal method (KDMAge) and phenotypic biological age (PhenoAge), with KDMAgeAccel and PhenoAgeAccel defined as the residuals of chronological age minus KDMAge and PhenoAge, respectively. We assessed the impact of accelerated biological aging on life expectancy in patients with IBD through survival analysis. Additionally, we examined genetic susceptibility and its potential mediating effects on the association between biological aging and IBD.
Findings: In the UK Biobank, accelerated biological aging was associated with an increased risk of IBD (KDMAgeAccel: HR 1.22, 95% CI 1.13-1.32; PhenoAgeAccel: HR 1.57, 95% CI 1.46-1.69). This association was further validated in the AoU cohort, where PhenoAgeAccel was also linked to an elevated risk of IBD (HR 1.57, 95% CI 1.18-2.09). An additive interaction was observed between accelerated biological aging and genetic risk for IBD. Individuals with both high genetic risk and accelerated aging exhibited the highest risk of developing IBD (KDMAgeAccel: HR 1.36, 95% CI 1.20-1.53; PhenoAgeAccel: HR 1.59, 95% CI 1.41-1.79). Life expectancy analysis indicated that IBD patients with accelerated biological aging experienced a significant reduction in life expectancy, with an average decrease of 1.36 years (KDMAgeAccel) and 1.95 years (PhenoAgeAccel). Mediation analyses suggested that accelerated biological aging partially mediated the protective effects of dried fruit and cooked vegetables on the risk of developing IBD. Results from multistate modelling showed that PhenoAgeAccel was also significantly associated with an increased risk of IBD occurrence to mortality (HR 1.44 [95% CI 1.17-1.77]).
Interpretation: Biological aging is significantly associated with the risk of IBD and its subtypes, especially in individuals with high genetic susceptibility, and it reduces life expectancy in these patients. Identifying individuals with accelerated biological aging can serve as a marker for the effective prevention and management of IBD.
Integrated Whole Genome and Transcriptome Sequencing as a Framework for Pediatric and Adolescent AML Diagnosis and Risk Assessment
Authors: Wang Lu , Voss Rebecca , Pastor Victor , Cardenas Maria , Kumar Priyadarshini , Maciaszek Jamie , Namwanje Maria , Ma Jing , Neary Jennifer , Jin Meiling , Umeda Masayuki , Wilkinson Mark , Payne-Turner Debbie , Eldomery Mohammad , Ma Jingqun , Gu Jiali , Dalton James , Melton Samantha , Liu Yen-Chun , Foy Scott , Rusch Michael , Wheeler David , Zhang Jinghui , Nichols Kim , Karol Seth , Inaba Hiroto , Ribeiro Raul , Rubnitz Jeffrey , Klco Jeffery 
Published: January 2025

Abstract

Pediatric acute myeloid leukemia (AML) exhibits distinct genetic characteristics, including unique driver alterations and mutations with prognostic and therapeutic significance. Emerging rare, recurrent genetic abnormalities and their associations with outcomes emphasize the need for high-throughput molecular diagnostic tools. Whole genome sequencing (WGS) reliably detects key AML biomarkers such as structural variants, mutations, and copy number alterations. Whole transcriptome sequencing (WTS) complements WGS by uncovering oncogene expression patterns, allele-specific expression, and gene expression signatures. In this study, we describe an integrated WGS and WTS clinical workflow for routine pediatric AML diagnosis and present a systematic evaluation of its application compared to conventional cytogenetics and standard molecular diagnostic methods. Our findings demonstrate that the integrated WGS and WTS (iWGS-WTS) approach improves the identification of clinically relevant genetic alterations, enhancing precise disease classification and risk assessment. Moreover, with advancements in workflow and bioinformatics pipelines, the testing turnaround time can be optimized to meet the demands of clinical decision-making, positioning iWGS-WTS as a practical and superior alternative to traditional diagnostic methods in pediatric AML management.
A Novel Mmachc c.80A&gt;G Mouse Model Reveals the Role of Mitochondrial Dysfunction in Skeletal Muscle in Combined Methylmalonic Acidemia and Homocystinuria
Authors: Ma Wenhao , Mao Yingying , Niu Hongze , Wu Zhijie , Zheng Ping , Zhao Tianyi , Zhang Cengceng , Yu Shuangqing , Zhou Jianfang , Ren Zipei , Cheng Peidi , Zhang Lina , Xie Lina , Dong Zheyue , Xia Yan , Sheng Wang , Feng Zhichun , Dong Xiaoyan , Chen Qian , Wu Xiaobing 
Published: January 2025

Abstract

Combined methylmalonic acidemia and homocystinemia (cblC) is an autosomal recessive disorder characterized by aberrant organic acid metabolism. The c.80A > G mutation in the MMACHC gene has been documented in numerous studies and linked to cblC phenotypes. However, this mutation's pathogenic mechanisms remain elusive, as it has not yet been validated through functional studies. In a previous study, we developed a murine model with the Mmachc c.80A > G mutation to elucidate the intricacies of the cblC disorder. Our current investigation delves deeper into the cblC mouse model, revealing persistently elevated levels of methylmalonic acid (MMA) and homocysteine (Hcy) in blood, urine, and tissues. This phenomenon can be attributed to diminished expression of the Mmachc. The survival rate of these model animals was markedly compromised, with altered blood biomarkers and imaging abnormalities mirroring the clinical manifestations of cblC. Further exploration revealed that the accumulation of MMA and Hcy in tissues is not only a consequence of B12-processing but also contributes to mitochondrial dysfunction, which is particularly pronounced in skeletal muscle. Concurrently, the c.80A > G mouse model exhibited disruption of mitochondrial autophagy, resulting in mitochondrial proliferation and impaired ATP metabolism. Notably, MMA emerged as a pivotal factor in cellular energy stress, damage to the mitochondrial respiratory chain and ATPase activity. Collectively, these findings indicate that the c.80A > G mutation plays a significant role in the mitochondrial pathogenesis of cblC by inducing intron retention, thereby providing novel insights into the underlying disease mechanism.
Microstructural Damage to the Optic Nerve with Vigabatrin Treatment in West Syndrome: A DTI Study
Authors: Hu Junjie , Chen Li , Zhang Gongwei , Fang Yu , Zhang Huiting , Li Yilian , Liao Jianxiang , Zhao Cailei 
Published: January 2025

Abstract

Objective: This study aimed to assess the side effects of vigabatrin (VGB), focusing on optic nerve fiber damage in children with West syndrome, using DTI imaging parameters.
Method: Clinical data and DTI images were analyzed, recording FA and ADC values for both optic nerves. ROC curves were used to determine fractional anisotropy thresholds for optic nerve damage.
Results: Thirty-five children with West syndrome (0.3-22 months old, male: female = 19:16) were divided into three groups: (1) VGB and other anti-seizure medications (ASMs) with symmetrical thalamus abnormalities, (2) VGB and ASMs without thalamus abnormalities, and (3) control group with other ASMs. FA values in group 1 were significantly lower than in group 3 (P < 0.05), and FA values increased after VGB discontinuation (P < 0.05). The ROC analysis showed a cut-off score of 304 with 63.6% sensitivity and 100% specificity for detecting optic nerve damage.
Conclusion: FA values are effective imaging markers for detecting VGB-induced optic nerve damage in West syndrome, especially when thalamus abnormalities are present. FA values significantly improve after stopping VGB treatment.
Ferroptosis-mediated primary open-angle glaucoma: Insight from gene signature and identification of potential small-molecule drugs
Authors: Zhou Xiaoyu , Zhang Xinyue , Xu Jiahao , Wu Ping , Duan Xuanchu 
Published: January 2025

Abstract

Background
Recent studies have found that ferroptosis may be involved in the process of trabecular meshwork injury in glaucoma. This study aims to reveal ferroptosis-related gene signature in primary open-angle glaucoma (POAG) and identify small molecule drugs as new direction of therapy.
Methods
Ferroptosis-related indicators in POAG patients and chronic ocular hypertension (COH) rats were detected by ELISA kits. The dataset (GSE27276) from GEO database and ferroptosis-related genes from FerrDb were downloaded for analysis. Small molecule drugs targeting ferroptosis-related signature components were predicted via CMap database and CB-Dock2. H2O2-induced human trabecular meshwork cells (HTMCs) oxidative stress model was constructed to validate the expression of hub genes and efficacy of drugs. Digoxin was made into eye drops to verify its intraocular pressure (IOP) lowering effect in vivo.
Results
Ferroptosis levels were enhanced in POAG patients and COH eyes of rats. A total of 14 ferroptosis-related differentially expressed genes were identified. PPI analysis and in vitro experiments showed HBA1, SLC2A3 and SCD played an important role in ferroptosis-mediated POAG. CMap and molecular docking indicated that ATPase inhibitors digoxin might be considered as potential therapeutic drugs for POAG. Digoxin administration alleviated H2O2-induced HTMCs ferroptosis and lowered IOP of COH eyes.
Conclusions
This study clarified the ferroptosis-related gene signature in the pathogenesis of POAG, which provide a theoretical basis for the prevention and early diagnosis of POAG. Translation of specific small molecule drugs will propose new ideas for therapy of POAG.
Impacts of anthropogenic activities on land use land cover change dynamics in the Ogun River Basin, Nigeria
Authors: Ojelabi Oluwatoyin Khadijat , Lawin Agnidé Emmanuel , Amichiatchi N'da Jocelyne , Oluwasemire Kolapo Olatunji 
Published: January 2025

ABSTRACT
In this research, the impacts of past (1984–2020) and future (2040–2060) changes in Ogun River Basin's land use land cover (LULC) dynamics were studied. LULC changes were classified using the Random Forest algorithm in Google Earth Engine on Landsat imagery from 1984, 2000, and 2020 epochs. The multi-layer perceptron-neural network and Markov Chain in the Land Change Modeler were used to predict the LULC maps under various future scenarios. The results revealed significant alterations in the LULC pattern. Forests, wetlands, and water bodies decreased between the epochs 1984 and 2020 by −29.0%, −58.3%, and −84.5%, respectively, whereas farmland and built-up areas expanded by 104 and 20%, respectively. The Business-as-Usual scenario is anticipated to result in an increase in farmland of 3.73% in 2040 and 3.89% in 2060, respectively, at the detriment of forest cover and wetland areas, which are projected to decrease. Under the afforestation scenario, the forest is predicted to expand at the expense of farmland and built-up areas, which are anticipated to expand by 6% (2040) and 8% (2060), respectively. These results, in combination with those derived from the Markov model, provide the necessary evidence base to support land use planning and the future-proofing of sustainable water resources management strategies in the basin.
Evaluating the Effectiveness of Resilience Training among Nursing Professionals in Saudi Arabia
Authors: Alanazi Hanan Qayyadh , Alreshidi Nashi Masnad , Alrimali Afaf Mufadhi , Alshammari Wadida Darwish , Elsharif Nashwa Essam , Teodosio Maria Theresa Hermano , Gonzales Kristine Angeles , Gaspar Fe Baltazar , Tabungar Debora Malibago , Alshammri Miad Ayed 
Published: January 2025

Introduction
Nursing is a demanding profession that calls for resilience and stress management. Nevertheless, many nurses do not receive adequate training in these areas. This study aimed to evaluate the impact of resilience training programs on work-related stress and resilience levels among nurses in Saudi Arabia.

Methods
A quasi-experiment intervention study employing pre- and post-test approaches was conducted from January to March, 2024, to assess a resilience training program for nurses in Hail, Saudi Arabia. Fifty nurses from sixteen hospitals were divided into two groups in the stratified random sample: the intervention group (n = 25) and the control group (n = 25). Data was collected using an online survey at baseline and three months post-training, with the Connor-Davidson Resilience Scale and the Expanded Nursing Stress Scale employed for measuring outcomes. The paired samples t-tests for statistical analysis were performed using the IBM SPSS Statistics 29.0.

Results
Significant improvements were noted in the intervention group, with resilience sores improving to 79.50 from 77.12 (p = 0.015) and stress levels decreasing to 63.54 from 65.08 (p = 0.020). No significant changes were noted in the control group.

Conclusion
Findings indicate that resilience training programs can significantly enhance resilience levels and reduce work-related stress. These results underscore the importance of implementing structured resilience training to better equip nurses for the challenges of their demanding profession.
Análisis de las prácticas de la responsabilidad social empresarial de las micro y pequeñas empresas de Puerto Vallarta, Jalisco
Authors: Becerra Bizarrón Manuel Ernesto 
Published: January 2025

Este estudio examina la adopción de prácticas de Responsabilidad Social Empresarial (RSE) en micro y pequeñas empresas (MIyPES) de Puerto Vallarta, Jalisco. El objetivo principal fue analizar la implementación de RSE en tres áreas: social, económica y medioambiental. Utilizando un enfoque correlacional y no experimental, se recolectaron datos mediante encuestas a 432 administradores, dueños y gerentes de empresas. Los datos se analizaron con la utilización del software SPSS y el programa AMOS para llevar a cabo análisis factoriales tanto exploratorios como confirmatorios. Los resultados indicaron que el 60% de la varianza total fue explicada por tres factores claves, demostrando que las MIyPES que aplican prácticas de RSE mejoran sus relaciones tanto con los empleados como con la comunidad. Además, la implementación de RSE también incrementa su sostenibilidad y competitividad en el sector. El análisis de fiabilidad mostró altos niveles de consistencia interna, con valores de 0.933, 0.910 y 0.853 para las dimensiones social, económica y medioambiental, respectivamente. El estudio confirma que la incorporación de RSE en las políticas y planes estratégicos de las MIyPES no solo es un compromiso ético, sino una estrategia que refuerza el rendimiento empresarial.
Endoscopic evaluation of the feline tympanic bulla is feasible for detection of epithelial remnants following ventral bulla osteotomy in a cadaveric model
Authors: Williams Rachel W. , Regier Penny J. , Pathak Nirali , Solari Francesca P. , Ham Kathleen M. , Bertran Judith , Case J. Brad 
Published: January 2025

Abstract

OBJECTIVE
To evaluate endoscopy for detection of epithelial remnants within the feline tympanic bulla following ventral bulla osteotomy (VBO) and to report the most likely locations within the bulla for epithelial remnants to occur.

METHODS
This was a single-center experimental cadaveric study. 10 VBO procedures were performed by 5 board-certified surgeons in 5 feline cadavers. Following completion, a 1.9-mm, 30° rigid endoscope was inserted into the rostral, caudal, dorsal, lateral, and medial section, and images were acquired. A blinded observer evaluated the endoscopic images for epithelial remnants, and the percentage of the section containing epithelial remnants was measured. The mean and SD of distribution of epithelial remnants were calculated for each section.

RESULTS
Remnant epithelium was noted in at least 1 section in each cadaver. The dorsal section was the most common to have epithelial remnants identified (10 of 10), whereas the lateral (5 of 10) and medial (5 of 10) were the least common. The lateral section contained the most epithelial remnants (6.9 ± 11.42%), whereas the caudal section contained the least (1.2 ± 1.42%).

CONCLUSIONS
The use of a 1.9-mm, 30° endoscope was feasible for evaluation and identification of epithelial remnants in a feline tympanic bulla following VBO. Epithelial remnants were consistently identified in all sections with endoscopic assistance.

CLINICAL RELEVANCE
Endoscopy following completion of a VBO may be considered to ensure complete removal of epithelium.
Reconciling a Fresh Start with Personal Data: The Challenge of EU Insolvency Discharge
Authors: Bolzanas Darius , Jokubauskas Remigijus 
Published: January 2025

Abstract
Designed to provide a fresh start, the discharge procedure relieves debtors of pre-bankruptcy debts, allowing them to resume economic activity. Attainment of this aim is also linked with the processing of the debtor’s personal data during and even after the discharge procedure is completed. The processing of the debtor’s personal data in the discharge procedure is subject to the general rules of protection of personal data in the European Union law. This article analyzes what the legal basis for processing of debtor’s personal data is during and after the discharge procedure. It also deals with the question whether a proper balance between the principle of publicity of the discharge procedure and the main goals of the processing of personal data (lawful processing of personal data, data minimization principle) are achieved under the current legal framework of the European Union. To show the practical problems in this area, the article focuses on the recent case law of the Court of Justice of the European Union, which reveals the problems to achieve the proper balance between the different aims of the discharge of debt procedure and processing of personal data.
The Self-Restrained Positive Legislator: Assessing the Constitutional Review of Legislative Omissions in Lithuania
Authors: Pūraitė-Andrikienė Dovilė , Valuta Andrius 
Published: January 2025

Abstract
The function of constitutional courts has undergone significant transformation in recent decades. More frequently, constitutional courts, instead of dealing with existing legislation, assume the role of assistants to the legislator. Some of these courts also control the absence of legislation or legislative omissions. This article seeks to explore the approach of the Lithuanian Constitutional Court in handling cases of legislative omission, particularly focusing on its efforts to strike a balance between respecting the autonomy of law-making institutions and upholding the supremacy of the Constitution. The text addresses the extent to which the Constitutional Court has adhered to the principle of self-restraint, ensuring that its interventions do not unduly encroach upon the prerogatives of law-making institutions. To reach this, the following tasks are undertaken and dealt with: 1) to shed light on the concept and typology of legislative omissions in comparative constitutional law, as well as the trends of their investigation (including statistical data) in European countries; 2) to analyse the official constitutional doctrine formulated by the Lithuanian Constitutional Court on the concept of legislative omissions and the scope of the Court’s power to examine legislative omissions; 3) to analyse the approach of the Lithuanian Constitutional Court to legislative omissions from a quantitative point of view, i.e. to examine the statistics of this Court in dealing with legislative omissions and the reasons for its changes.
Institutional Components Affecting the Internationalization of Higher Education: A Case Study on Estonia
Authors: Joamets Kristi , Vasquez Maria Claudia Solarte 
Published: January 2025

Abstract
A comprehensive understanding of higher education internationalization remains elusive within the European context, despite the guidance offered by the European Union (EU) through policy principles, notably the Bologna Process. As a result, Member States face challenges in developing and implementing effective internationalization strategies. Typically, these prove unsustainable due to their overly ambitious and broad focus, leading to inefficiencies such as inadequate allocation of resources, and to fragmented approaches that often fail to produce cohesive, functional systems. This paper argues that establishing clear definitions, priorities and coordinated action plans involving key stakeholders could enhance the formulation of more feasible and impactful regulatory strategies, facilitating a smooth internationalization in the European Higher Education Area (EHEA). The opposite would increase the likelihood of wasteful investments and mismanagement, affecting the stability, reliability, and sustainability of advancements in this area. This article contributes to the delineation of a higher education internationalization model drawing from an examination of Estonian institutional policies and regulatory trends. Through a partial institutional review, it explores the challenges, opportunities, and prospects inherent to cultiva ting a well defined, principled and comprehensive internationalization approach, emphasizing the necessity of meaningful stakeholder involvement for the creation of reflective regulatory frameworks to ensure the attainment of tangible and measurable goals.
Research Data Management Within the EU Data Policy Framework
Authors: Milčiuvienė Saulė , Amilevičius Darius , Raškinis Gailius 
Published: January 2025

Abstract
The rapid digitalization has significantly influenced EU research policy, particularly in open science and research data management. While free access to research data is integral to open science, the broader concept of research data management extends beyond mere accessibility. The EU has introduced various regulations to address the evolving challenges of digital technologies, balancing innovation with fundamental legal principles such as privacy and intellectual property protection. Although no single legal framework is dedicated to research data, multiple EU policies directly impact its management. The Open Data Directive emphasizes the reuse of publicly funded research data, promoting open access while safeguarding intellectual property and privacy rights. The Data Governance Act complements this by creating a framework for reusing protected public sector data, balancing openness with the need to protect commercial, intellectual, and personal data. The Data Act primarily focuses on private sector data and facilitates data sharing across businesses and sectors, particularly in the domain of the Internet of Things, while promoting interoperability and fair contractual terms. Despite these robust EU-level frameworks, Lithuania’s national policies lag behind, particularly in addressing the growing needs of artificial intelligence innovation.