bluuebunny/crossref_metadata_2025_split huggingface dataset:
Antibiotic potentiation and inhibition of cross-resistance in pathogens associated with cystic fibrosis
Authors: Kadeřábková Nikol , Furniss R. Christopher D. , Maslova Evgenia , Eisaiankhongi Lara , Bernal Patricia , Filloux Alain , Landeta Cristina , Gonzalez Diego , McCarthy Ronan R. , Mavridou Despoina A.I. 
Published: August 2023

ABSTRACTCritical Gram-negative pathogens, likePseudomonas,StenotrophomonasandBurkholderia, have become resistant to most antibiotics. Complex resistance profiles together with synergistic interactions between these organisms increase the likelihood of treatment failure in distinct infection settings, for example in the lungs of cystic fibrosis patients. Here, we discover that cell envelope protein homeostasis pathways underpin both antibiotic resistance and cross-protection in CF-associated bacteria. We find that inhibition of oxidative protein folding inactivates multiple species-specific resistance proteins. Using this strategy, we sensitize multi-drug resistantPseudomonas aeruginosato β-lactam antibiotics and demonstrate promise of new treatment avenues for the recalcitrant pathogenStenotrophomonas maltophilia. The same approach also inhibits cross-protection between resistantS. maltophiliaand susceptibleP. aeruginosa, allowing eradication of both commonly co-occurring CF-associated organisms. Our results provide the basis for the development of next-generation strategies that target antibiotic resistance, while also impairing specific interbacterial interactions that enhance the severity of polymicrobial infections.
Multifunctionality of V-type ATPase during asexual growth and development ofPlasmodium falciparum
Authors: Shadija Neeta , Dass Swati , Xu Wei , Ke Hangjun 
Published: August 2023

AbstractV-type ATPases are highly conserved hetero-multi-subunit proton pumping machineries found in all eukaryotic organisms. They use ATP hydrolysis to pump protons, acidifying intracellular or extracellular compartments, and are thus crucial for various biological processes. Despite being evolutionarily conserved in malaria parasites, this proton pump remains understudied. To understand the localization and biological function of V-type ATPase in the deadliest human malaria parasitePlasmodium falciparum, we utilized CRISPR/Cas9 to endogenously tag the subunit A of the V1domain at the C-terminus. V1A (PF3D7_1311900) was tagged with a triple hemagglutinin (3HA) epitope and TetR-DOZI-aptamers for conditional expression under the regulation of anhydrotetracycline. Through immunofluorescence assays, we identified that V-type ATPase was expressed throughout the intraerythrocytic developmental cycle and was mainly localized on the digestive vacuole and plasma membrane. Immuno-electron microscopy further revealed that V-type ATPase was also localized on secretory organelles, such as rhoptries in merozoites. Knockdown of V1A led to cytosolic pH imbalance and blockage of hemoglobin digestion in the digestive vacuole, resulting in an arrest of parasite development in the trophozoite stage and, ultimately, parasite demise. Using BN-PAGE/Western blot, we detected a large molecular weight complex (∼ 1.0 MDa) corresponding to the total molecular weights of V1and Vodomains. The complex was readily disrupted by the V-type ATPase specific inhibitor Bafilomycin A1, but not by low glucose conditions or treatment with chloroquine. Together, our data suggest that V-type ATPase is localized on several subcellular compartments inP. falciparumand plays critical roles to support malaria parasites to grow and replicate inside red blood cells.
Experience-dependent information routing through the basolateral amygdala
Authors: Antonoudiou Pantelis , Stone Bradly , Colmers Phillip L.W. , Evans-Strong Aidan , Walton Najah , Weiss Grant , Maguire Jamie 
Published: August 2023

AbstractThe basolateral amygdala (BLA) is an emotional processing hub and is well-established to influence both positive and negative valence processing. Selective engagement of a heterogeneous cell population in the BLA is thought to contribute to this flexibility in valence processing. However, how this process is impacted by previous experiences which influence valence processing is unknown. Here we demonstrate that previous positive (EE) or negative (chronic unpredictable stress) experiences differentially influence the activity of specific populations of BLA principal neurons projecting to either the nucleus accumbens core or bed nucleus of the stria terminalis. Using chemogenetic manipulation of these projection-specific neurons we can mimic or occlude the effects of chronic unpredictable stress or enriched environment on valence processing to bidirectionally control avoidance behaviors and stress-induced helplessness. These data demonstrate that previous experiences influence the responsiveness of projection-specific BLA principal neurons, biasing information routing through the BLA, to govern valence processing.
Comparative single-nucleus RNA-seq analysis captures shared and distinct responses to beneficial and pathogenic microbes in roots
Authors: Yang Qiuhua , Li Zhuowen , Guan Kaixiang , Liu Zhijian , Huang Ancheng , Zhai Jixian , Long Yanping , Song Yi 
Published: August 2023

AbstractDistinguishing and differentially responding to beneficial and pathogenic microbes are fundamental for plants to maintain microbiome homeostasis and promoting plant fitness. Using a recently developed protoplast-free single-nucleus RNA-seq approach, we generated single-cellular atlas of root responses to beneficial and pathogenic microbes. Notably, we identified triterpene biosynthesis as a novel cell type specific response to root pathogens and genetically confirmed the role of triterpene biosynthesis in regulating beneficial/pathogenic microbe ratios in a two-strain mixed community. Our results provide novel insights and vital resources for further elucidating novel regulators of beneficial and pathogenic microbe colonization and microbiome homeostasis.
Digital marketing adoption by small travel agencies: a comprehensive PLS-SEM model using reflective and higher-order formative constructs
Authors: Sharma Ankita , Sharma Swati 
Published: August 2023

PurposeThis study examines the vital factors that influence digital marketing adoption among small travel agencies (brick-and-mortar) and the impact of digital marketing adoption on organizational performance modeled as a formative-formative HOC.Design/methodology/approachThis empirical study builds on diffusion of innovation (DOI) theory and technology-organization-environment (TOE) framework to identify the factors of digital marketing adoption by small travel agencies. Data were collected from 226 small travel agents in India using purposive and snowball sampling. Partial least square-structural equation modelling (PLS-SEM) is used to analyze the reflectively and formatively measured constructs.FindingsThe findings reveal significant constructs of digital marketing adoption by small travel agencies. The study also establishes the positive impact of digital marketing adoption on organizational performance. Trust partially mediates the impact of technological factors (relative advantage, security concerns) and organizational factor (knowledge) on digital marketing adoption.Research limitations/implicationsThe study provides methodological contribution to the literature by applying confirmatory composite analysis (CCA) approach in PLS-SEM. The study contributes to the literature by integrating DOI theory and TOE framework. The study enriches the literature on trust as it recognizes the crucial role of trust as a mediating construct.Practical implicationsThe study provides useful implications to marketing practitioners of small travel agencies. The study shows strong predictive capacity and can be generalized throughout diverse samples.Originality/valueThe study adds value to the literature as it explores the digital marketing adoption among the under researched small travel agencies by integrating DOI-TOE theory. The study uniquely proposes and validates organizational performance as a formative-formative higher-order construct.
The role of artificial intelligence in improving the omnichannel customer experience
Authors: Calvo Ana Valeria , Franco Ana Dolores , Frasquet Marta 
Published: August 2023

PurposeThis study aims to explore the role that artificial intelligence (AI) systems could play in configuring and enhancing the omnichannel customer experience (OCE).This paper aims to pave the way to better understand the intersection between these two novel topics through perspectives and associated interpretations from managers', consultants' and consumers' beliefs, experiences and thoughts.Design/methodology/approachThe study adopts an explorative inductive design. Data from 41 in-depth interviews with high-level retail managers (12), AI consultants (3) and omnichannel consumers (26) was analyzed using grounded theory methodology.FindingsThe study's results revealed that, when AI systems are implemented in the omnichannel experience, some dimensions of the OCE change in relevance. The findings show that some OCE dimensions are easier to relate with experiential elements of the omnichannel experience, such as personalization, consistency and flexibility. In contrast, integration and connectivity are perceived as internal retailer capabilities that enable the omnichannel strategy. Consumers' data also show differences in the omnichannel customer journeys for the product categories of clothes, electronics and furniture.Originality/valueThis study presents insights on the impact of AI on OCE from top-retail managers', consultants' and consumers' perspectives. This choice allowed researchers to explore and uncover interesting intersecting points and examine issues related to omnichannel experience and AI systems implementation, providing guidance for future research.
Impact of COVID-19 stress on employee performance and well-being: role of trust in management and psychological capital
Authors: Pradhan Rabindra Kumar , Panda Madhusmita , Hati Lopamudra , Jandu Kailash , Mallick Manolina 
Published: August 2023

Purpose
The COVID-19 pandemic presents an unprecedented case of organisation–environment interaction in the occupational settings. Using Palmer and colleagues (2003) model of work stress, this study aims to examine the influence of COVID-19 stress on employee performance and psychological well-being. Furthermore, drawing on conservation of resources theory (Hobfoll, 1989), the role of trust in management and psychological capital as moderators has also been explored.

Design/methodology/approach
Data were collected from the service sector employees (N = 507) using purposive sampling technique.

Findings
Structural equation modelling analysis revealed the negative impact of COVID-19 stress on employee performance and psychological well-being; moderated by trust in management and psychological capital, respectively.

Research limitations/implications
The proposed conceptual model contributes to the stress management literature by providing an understanding of how COVID-19-related stress impacts employee behaviour in organisational context. The present study considers the COVID-19 stress as bio-psycho-socioeconomic challenge that impacts and determines nearly all the activities of the individuals, groups and organisations at a global scale.

Practical implications
The findings of the studies offer several managerial implications pertaining to performance and well-being at workplace especially during such crisis characterised by the elements of uncertainty and ambiguity. Identification of the personal resources would prove beneficial for the management in devising and implementing customised interventions for employees and the organisation. Since the pandemic has impacted each individual differently, human resource managers should remind their employees to be vigilant about self-care and take advantage of their health care benefits, particularly with regards to mental health.

Originality/value
The proposed conceptual model contributes to the workplace stress management literature by providing an understanding of how COVID-19-related stress impacts employee outcomes in the organisational context. Furthermore, identifying personal resources would prove beneficial for the management in devising and implementing customised interventions at the workplace.
Exploring overnight social care for older adults: a scoping review
Authors: Boyle Naomi , Seddon Diane , Toms Gill 
Published: August 2023

Purpose
Demand for care at home is growing because of the increase in life expectancy, an ageing population and the chronic conditions that often accompany longevity. Daytime care at home services have been widely reported on, but less is known about overnight care at home. This paper aims to gather evidence about overnight social care for older adults in their homes.

Design/methodology/approach
Recent studies were identified through searches in three electronic databases. Studies published in English between January 2016 and June 2022 exploring overnight care at home for older adults were eligible for inclusion. An additional Google search identified home care services within the UK currently providing overnight support.

Findings
The review retrieved five relevant papers, highlighting the paucity of research in this area. A narrative review of the literature identified common themes that suggested domiciliary night care staff play an integral role in meeting the overnight care and support needs of older adults who wish to be cared for at home. Despite the limited evidence base in this area, the Google search for UK domiciliary services who provide overnight support identified several active programmes.

Originality/value
To the best of our knowledge, this is the first scoping review exploring the provision of overnight social care to older adults in their own homes. The review highlights the need for further research to inform commissioning and practice development.
Permeability evolution in fine‐grained Aji granite during triaxial compression experiments
Authors: Sueyoshi Kazumasa , Katayama Ikuo , Sawayama Kazuki 
Published: August 2023

AbstractTriaxial compression experiments were carried out on samples of fine‐grained Aji granite to measure the evolution of permeability during deformation prior to failure under confining pressures of 20 and 40 MPa. During the initial stages of deformation, a small decrease in permeability was observed, due to the closure of pre‐existing microcracks; permeability then increased with increasing differential stress. During deformation, permeability varied by up to two orders of magnitude, and we observed a small pressure dependence, with a larger variation observed at 20 MPa than at 40 MPa. This suggests that more cracks developed during brittle deformation under the lower confining pressure. The observed increase in permeability during our experiments was approximately proportional to inelastic volumetric strain, which corresponded to the volume of dilatant cracks. On the other hand, prior to brittle failure, we observed a further increase in permeability that was greater than the inelastic volumetric strain, suggesting crack aperture opening accelerated at this stress level (>∼80%). The permeability enhancement resulting from the crack dilation affects the gas‐sealing capacity of the fluid‐saturated caprock. Our experimental findings would be beneficial for the safety assessment in applications such as the long‐term storage of various gaseous wastes.
Gustatory responsiveness in Vespula germanica workers: exploring the interplay between sensory perception and task specialization
Authors: Mattiacci Analía , Goñalons Carolina Mengoni , Masciocchi Maité , Corley Juan C. 
Published: August 2023

AbstractWorkers’ task specialization and division of labor are critical features of social insects’ ecological success. It has been proposed that the division of labor relies on response threshold models: individuals varying their sensitivity (and responsiveness) to biologically relevant stimuli and performing a specific task when a stimulus exceeds an internal threshold. In this work, we study carbohydrate and protein responsiveness and their relation to worker task specialization in Vespula germanica, an invasive social wasp. The sucrose and peptone responsiveness of two different subcastes, preforagers and foragers, was determined by stimulating the antenna of the wasps with increasing concentrations of the solution and quantifying whether each concentration elicited a licking response. We studied responsiveness in five different ways: (1) response threshold, (2) concentration 50 (concentration to which at least 50% of wasps responded), (3) maximum response, (4) mean scores and (5) median scores. Our results suggest that V. germanica foragers are more sensitive to sucrose (lower thresholds) than preforager workers. However, we found no differences for peptone thresholds (i.e., a protein resource). Nonetheless, this is the first study to investigate response thresholds for protein resources. The intercaste variation in sucrose responsiveness shown in our work contributes to the existing knowledge about response threshold theory as a mechanism for task specialization observed in V. germanica.
Predictors of lung injury during durvalumab maintenance therapy following concurrent chemoradiotherapy in unresectable locally advanced non‐small cell lung carcinoma
Authors: Hirama Nobuyuki , Yamamoto Masaki , Nagaoka Satoshi , Segawa Wataru , Sugimoto Chihiro , Nagayama Hirokazu , Hiro Shuntaro , Kajita Yukihito , Maeda Chihiro , Kubo Sousuke , Seki Kenichi , Nagahara Yoshinori , Teranishi Shuhei , Tashiro Ken , Hara Yu , Kobayashi Nobuaki , Watanabe Shigenobu , Kudo Makoto , Kaneko Takeshi 
Published: August 2023

AbstractBackgroundBased on the results of the PACIFIC trial, maintenance with durvalumab has emerged as the standard treatment following concurrent chemoradiotherapy in patients with unresectable locally advanced non‐small cell lung carcinoma (NSCLC). However, adverse events attributed to durvalumab, especially lung injuries, including immune‐related adverse events, and radiation pneumonitis, are concerning. This study retrospectively investigated the factors related to lung injury in patients receiving the PACIFIC regimen.MethodsPatients with unresectable locally advanced NSCLC who received durvalumab maintenance therapy following concurrent chemoradiotherapy at Yokohama City University Medical Centre between July 2018 and March 2022 were included. Clinical data, volume of normal lung receiving 20 or 5 Gy or more (V20 or V5), planning target volume (PTV), and relative lung parenchyma volume in emphysematous lung receiving 20 or 5 Gy or more (RLPV20 or 5; V20 or V5/100‐percentage of low‐attenuation volume) were evaluated.ResultsPerformance status (PS), V20, V5, PTV, RLPV20, and RLPV5 were significantly higher in the lung injury group in the univariate analysis. Furthermore, RLPV20 was the most significant factor in the lung injury group in the multivariate analysis comprising PS, PTV, V20, and RLPV20.ConclusionRLPV20 and RLPV5 are useful in estimating lung inflammation. RLPV20 could be considered the most reliable risk factor for maintenance therapy with durvalumab following concurrent chemoradiotherapy in patients with unresectable locally advanced NSCLC.
Sevoflurane suppresses the malignant progression of breast cancer via the hsa_circ_0000129/miR‐578/EPSTI1 axis
Authors: Zeng Xuehui , Zheng Qingqi , Wang Lang , Chen Tao , Lin Wenxin , Lin Quanyang 
Published: August 2023

AbstractBackgroundSevoflurane (Sev) is a commonly used volatile anesthetic that might suppress the process of breast cancer. Also, circular RNAs (circRNAs) have been reported to partake in the pathogenesis of breast cancer. Accordingly, this research was designed to investigate the mechanism of hsa_circ_0005962 on Sev‐mediated breast cancer development.MethodsSev was applied to treat breast cancer cells. Cell proliferation ability, migration, invasion, and apoptosis were detected using Cell Counting Kit‐8 (CCK‐8), 5‐ethynyl‐2′‐deoxyuridine (EdU), Transwell, and flow cytometry assay. Proliferating cell nuclear antigen (PCNA), Matrix metallopeptidase 9 (MMP9), B‐cell lymphoma‐2 (Bcl‐2)‐associated X protein (Bax), and Epithelial stromal interaction 1 (EPSTI1) were assessed using western blot assay. circ_0000129, microRNA‐578 (miR‐578), and EPSTI1 levels were determined using real‐time quantitative polymerase chain reaction (RT‐qPCR). Using bioinformatics software (Circinteractome and Targetscan), the binding between miR‐578 and circ_0000129 or EPSTI1 were predicted, and proved using dual‐luciferase reporter and RNA pull‐down assay. The biological roles of circ_0000129 and Sevoflurane on tumor growth were analyzed using a xenograft tumor model in vivo.ResultsSevoflurane blocked tumor cell proliferation, migration, invasion, and promoted apoptosis. Circ_0000129 and EPSTI1 expression were increased, and miR‐578 was decreased in breast cancer cells. Also, they presented an opposite trend in Sev‐treated tumor cells. Circ_0000129 upregulation might abolish Sev‐mediated tumor progression in vitro. Mechanically, circ_0000129 can affect EPSTI1 expression by sponging miR‐578. Sev might inhibit tumor growth by regulating circ_0000129 in vivo.ConclusionCirc_0000129 relieved Sev‐triggered suppression impacts on breast cancer development partly via the miR‐578/EPSTI1 axis, which provides a new mechanism for studying mediated therapy of breast cancer treatment.
Hepatocellular carcinoma in pregnancy: A systematic review
Authors: Marasciulo Francesco , Passerini Irene , Fichera Anna , Ferrari Federico , Odicino Franco E. , Prefumo Federico 
Published: August 2023

AbstractIntroductionHepatocellular carcinoma (HCC) is the most frequent primary malignant liver tumor and typically develops in the context of chronic liver disease, such as liver cirrhosis or chronic hepatitis B virus infection. Ultrasound evaluation, CT scan, and MRI are used to detect HCC. α‐fetoprotein (AFP) is a common marker used to detect HCC in the non‐pregnant population, which notoriously increases in pregnant women in relation to gestational age. Treatment is driven by the extent of the disease and the severity of underlying liver disease. Pregnancy may represent an obstacle to diagnosis and appropriate treatment of HCC. The aim of this descriptive systematic review was to describe the clinical features and maternal and neonatal outcomes of HCC in pregnancy.Material and methodsWe performed a systematic review of the literature about HCC diagnosed in pregnancy and the postpartum period, with signs or symptoms arising in pregnancy. We included case reports and case series describing the clinical features of women diagnosed with HCC, fibrolamellar variant of HCC, and mixed HCC and cholangiocarcinoma during pregnancy or the postpartum period (with onset of symptoms during pregnancy), from inception to March 2023. The study protocol was registered with the PROSPERO database (Registration number: ID CRD42021275584).ResultsWe identified 180 records. The articles included in this systematic review were 47 case reports and 5 case series, for a total of 63 pregnancies. The two most frequent predisposing conditions were hepatitis B virus infection (30/63; 47%) and liver cirrhosis (14/63; 22%). Ultrasound evaluation was the most used technique to detect HCC. AFP was higher than normal in 28/46 patients tested (61%). Surgical treatment was the most used therapy, both during pregnancy and after delivery. Twenty‐six patients (26/63; 42%) died within 6 months of diagnosis. Survival >24 months was 9% (4/46) in symptomatic and 29% (5/17) in asymptomatic women. No patient with cirrhotic liver survived more than 12 months. Thirty‐eight newborns were alive at 28 days of age (38/63; 61%).ConclusionsHepatocellular carcinoma in pregnancy is associated with a high risk of maternal and neonatal mortality. Diagnosis in asymptomatic high‐risk women or following abnormal maternal serum AFP screening is associated with better maternal outcomes.