bluuebunny/crossref_metadata_2025_split huggingface dataset:
NR4A1 silencing alleviates high-glucose-stimulated HK-2 cells pyroptosis and fibrosis via hindering NLRP3 activation and PI3K/AKT pathway
Authors: Li Jin-Meng , Song Zi-Hua , Li Yuan , Chen Han-Wen , Li Han , Yuan Lu , Li Jing , Lv Wen-Yue , Liu Lei , Wang Na 
Published: January 2025

BACKGROUND
The pathophysiology of diabetic kidney disease (DKD) is complex. Interfering with the processes of pyroptosis and fibrosis is an effective strategy for slowing DKD progression. Previous studies have revealed that nuclear receptor subfamily 4 group A member 1 (NR4A1) may serve as a novel pathogenic element in DKD; however, the specific mechanism by which it contributes to pyroptosis and fibrosis in DKD is unknown.
AIM
To investigate the role of NR4A1 in renal pyroptosis and fibrosis in DKD and possible molecular mechanisms.
METHODS
Streptozotocin 60 mg/kg was injected intraperitoneally to establish a rat model of DKD. Typically, 45 mmol/L glucose [high glucose (HG)] was used to activate HK-2 cells to mimic the DKD model in vitro . HK-2 cells were transfected with NR4A1 siRNA to silence NR4A1.
RESULTS
NR4A1 was elevated in renal tissues of DKD rats and HG-stimulated HK-2 cells. Concurrently, NOD-like receptor protein 3 (NLRP3) and phosphoinositide 3-kinase (PI3K)/protein kinase B (AKT) pathways were triggered, and pyroptosis and expression of fibrosis-linked elements was increased in vivo and in vitro . These alterations were significantly reversed via NR4A1 silencing.
CONCLUSION
Inhibition of NR4A1 mitigated pyroptosis and fibrosis via suppressing NLRP3 activation and the PI3K/AKT pathway in HG-activated HK-2 cells.
High expression of stearoyl-coenzyme A desaturase in colorectal cancer oncogenic functions and its potential as a therapeutic target
Authors: Wang Xiao-Wei , Huang Wan-Ying , Qin Kai , Zeng Da-Tong , Chen Zu-Yuan , Chi Bang-Teng , Tang Yu-Xing , Li Qi , Li Bin , Li Dong-Ming , He Rong-Quan , Huang Wei-Jian , Chen Gang , Tang Rui-Xue , Feng Zhen-Bo 
Published: January 2025

BACKGROUND
The stearoyl-coenzyme A desaturase (SCD ) gene influences colorectal cancer (CRC) pathogenesis, with its expression linked to tumor cell survival and resistance, necessitating further investigation into its role in CRC.
AIM
To explore the clinical and pathological significance of SCD expression in CRC tissues and to evaluate the affinity between nitidine chloride (NC) and SCD as a target.
METHODS
Multi-center high-throughput data related to CRC were integrated to calculate the standardized mean difference of SCD mRNA expression levels. Immunohistochemical staining results, Clustered Regularly Interspaced Short Palindromic Repeats knockout screening results of cell growth, and single-cell sequencing were employed to verify the significance of SCD expression in CRC. The clinical and pathological significance of SCD was assessed using pooled receiver operating characteristic curves, sensitivity, specificity, and likelihood ratios. The molecular mechanism of NC against CRC was clarified using the SwissTarget Prediction and functional enrichment, and molecular docking techniques were utilized to explore the targeting affinity between NC and SCD.
RESULTS
Data from 18 platforms, including 2482 CRC samples and 1334 non-cancerous colorectal tissue controls. SCD expression was significantly upregulated in CRC, with a standardized mean difference of 2.05 [95% confidence interval (CI): 1.69-2.41]. The area under the pooled receiver operating characteristic curve was 0.95 (95%CI: 0.92-0.96), with a sensitivity of 0.86 (95%CI: 0.81-0.90) and a specificity of 0.90 (95%CI: 0.87-0.93). Positive and negative likelihood ratios were 9.02 (95%CI: 6.49-12.51) and 0.15 (95%CI: 0.10-0.22), respectively. High SCD protein expression was noted in 208 CRC patients, significantly associated with vascular invasion (P < 0.001). At the single-cell level, SCD was significantly overexpressed in CRC cells (P < 0.001). A total of 33 CRC cell lines depended on SCD for growth. The potential mechanism of NC against CRC might involve modulation of the cell cycle, positioning SCD as a potential target for NC.
CONCLUSION
SCD promotes CRC cell growth and thus acts as an oncogenic factor, making it a potential therapeutic target for NC in CRC treatment.
Internal biliary diversion using appendix during liver transplantation for progressive familial intrahepatic cholestasis type 1: A case report
Authors: Song Jia-Qi , Zhou Tao , Luo Yi , Liu Yuan 
Published: January 2025

BACKGROUND
Progressive familial intrahepatic cholestasis type 1 (PFIC-1) is a genetic cholestatic disease causing end-stage liver disease, which needs liver transplantation (LT). Simultaneous biliary diversion (BD) was recommended to prevent allograft steatosis after transplantation, while increasing the risk of infection. Here, an attempt was made to perform BD using appendix to prevent bacterial translocation after LT.
CASE SUMMARY
An 11-month-old boy diagnosed with PFIC-1 received ABO compatible living donor LT due to refractory jaundice and pruritus. His mother donated her left lateral segment with a graft-to-recipient weight ratio of 2.9%. Internal BD was constructed during LT using the appendix by connecting its proximal end with the intrahepatic biliary duct and the distal end with colon. Biliary leakage was suspected on the 5th day after transplantation and exploratory laparotomy indicated biliary leakage at the cutting surface of liver. The liver function returned to normal on the 9th day post-operation and maintained normal during the 15-month follow-up. Cholangiography at 10 months after transplantation confirmed the direct secretion of bile into colon. Computerized tomography scan (4 months and 10 months) and liver biopsy (10 months) indicated no steatosis in the allograft. No complaint of recurrent diarrhea, infection or growth retardation was reported during follow-up.
CONCLUSION
Internal BD using appendix during LT is effective in preventing allograft steatosis and post-transplant infection in PFIC-1 recipients.
Comprehensive influence of individualized nutrition support and nursing strategy on rehabilitation of patients with liver cancer after operation
Authors: Zhang Xue-Li , Ma Hai-Yan 
Published: January 2025

BACKGROUND
Hepatocellular carcinoma ranks among the most prevalent malignant neoplasms. Surgical intervention constitutes a critical therapeutic approach for this condition. Nonetheless, postoperative recovery is frequently influenced by the patient's nutritional status and the quality of nursing care provided.
AIM
To examine the comprehensive impact of personalized nutritional support and nursing strategies on the postoperative rehabilitation of patients with liver cancer.
METHODS
In this study, a retrospective comparative analysis was conducted involving 60 post-operative liver cancer patients. The subjects were selected as subjects and divided into two groups based on differing nursing interventions, with each group comprising 30 patients. The control group received standard nutritional support and care, whereas the experimental group received individualized nutritional support and nursing strategies. The study aimed to evaluate the impact of individualized nutrition by comparing the rehabilitation indices, nutritional status, quality of life (QoL), and complication rates between the two groups.
RESULTS
The results showed that the recovery index of the experimental group was significantly better than that of the control group 2 weeks after surgery, and the average liver function recovery index of the experimental group was 85. significantly higher than that of the control group (73.67 ± 7.19). In terms of nutritional status, the serum albumin level and body weight stabilization rate of the experimental group were also significantly higher than those of the control group, which were 42.33 ± 2.4 g/L and 93.3%, respectively, compared with 36.01 ± 3.85 g/L and 76.7% of the control group. In addition, the average QoL score of the experimental group was 84.66 ± 3.7 points, which was significantly higher than that of the control group (70.92 ± 4.28 points). At the psychological level, the average anxiety score of the experimental group was 1.17 ± 0.29, and the average depression score was 1.47 ± 0.4, which were significantly lower than the 2.26 ± 0.42 and 2.57 ± 0.45 of the control group. This showed that patients in the experimental group were better relieved of anxiety and depression under the individualized nutrition support and nursing strategy. More importantly, the complication rate in the experimental group was only 10%, much lower than the 33.3% in the control group.
CONCLUSION
Personalized nutritional support and tailored nursing strategies significantly enhance the postoperative rehabilitation of liver cancer patients. Consequently, it is recommended to implement and advocate for these individualized approaches to improve both the recovery outcomes and QoL for these patients.
Predictive value of C-reactive protein, procalcitonin, and total bilirubin levels for pancreatic fistula after gastrectomy for gastric cancer
Authors: Yuan Jing-Long , Wen Xuan , Xiong Pan , Pei Li 
Published: January 2025

BACKGROUND
Gastric cancer is the most common malignancy of the digestive system and surgical resection is the primary treatment. Advances in surgical technology have reduced the risk of complications after radical gastrectomy; however, post-surgical pancreatic fistula remain a serious issue. These fistulas can lead to abdominal infections, anastomotic leakage, increased costs, and pain; thus, early diagnosis and prevention are crucial for a better prognosis. Currently, C-reactive protein (CRP), procalcitonin (PCT), and total bilirubin (TBil) levels are used to predict post-operative infections and anastomotic leakage. However, their predictive value for pancreatic fistula after radical gastrectomy for gastric cancer remains unclear. The present study was conducted to determine their predictive value.
AIM
To determine the predictive value of CRP, PCT, and TBil levels for pancreatic fistula after gastric cancer surgery.
METHODS
In total, 158 patients who underwent radical gastrectomy for gastric cancer at our hospital between January 2019 and January 2023 were included. The patients were assigned to a pancreatic fistula group or a non-pancreatic fistula group. Multivariate logistic analysis was conducted to assess the factors influencing development of a fistula. Receiver operating characteristic (ROC) curves were used to determine the predictive value of serum CRP, PCT, and TBil levels on day 1 post-surgery.
RESULTS
On day 1 post-surgery, the CRP, PCT, and TBil levels were significantly higher in the pancreatic fistula group than in the non-pancreatic fistula group (P < 0.05). A higher fistula grade was associated with higher levels of the indices. Univariate analysis revealed significant differences in the presence of diabetes, hyperlipidemia, pancreatic injury, splenectomy, and the biomarker levels (P < 0.05). Logistic multivariate analysis identified diabetes, hyperlipidemia, pancreatic injury, CRP level, and PCT level as independent risk factors. ROC curves yielded predictive values for CRP, PCT, and TBil levels, with the PCT level having the highest area under the curve (AUC) of 0.80 [95% confidence interval (CI): 0.72-0.90]. Combined indicators improved the predictive value, with an AUC of 0.86 (95%CI: 0.78-0.93).
CONCLUSION
Elevated CRP, PCT, and TBil levels predict risk of pancreatic fistula post-gastrectomy for gastric cancer.
Comparative study of cylindrical vs circular ring magnets for colonic anastomosis in rats
Authors: Zhang Miao-Miao , Shi Ai-Hua , Muensterer Oliver J , Uygun Ibrahim , Lyu Yi , Yan Xiao-Peng 
Published: January 2025

BACKGROUND
Magnetic compression anastomosis (MCA) offers a simple and reliable technique for inducing anastomoses at any point along the digestive tract. Evidence regarding whether the design of the MCA device influences the anastomosis effect is lacking.
AIM
To investigate any difference in the side-to-side colonic anastomosis effect achieved with cylindrical vs circular ring magnets.
METHODS
We designed cylindrical and circular ring magnets suitable for side-to-side colonic anastomosis in rats. Thirty Sprague-Dawley rats were randomly divided into a cylindrical group, circular ring group, and cylindrical–circular ring group (n = 10/group). Side-to-side colonic anastomosis was completed by transanal insertion of the magnets without incision of the colon. Operation time, perioperative complications, and magnet discharge time were recorded. Rats were euthanized 4 weeks post-operatively, and anastomotic specimens were obtained. The burst pressure and anastomotic diameter were measured sequentially, and anastomosis formation was observed by naked eye. Histological results were observed by light microscopy.
RESULTS
In all 30 rats, side-to-side colonic anastomosis was completed, for an operation success rate of 100%. No postoperative complications of bleeding and intestinal obstruction occurred, and the postoperative survival rate were 100%. The operation time, magnet discharge time, anastomotic bursting pressure, and anastomotic diameter did not differ significantly among the three designs (P > 0.05). Healing was similar across the groups, with gross specimens showing good anastomotic healing and good mucosal continuity observed on histological analysis.
CONCLUSION
This study found no significant difference in the establishment of rat side-to-side colonic anastomosis with the use of cylindrical vs circular ring magnets.
Healthcare professionals' knowledge, attitude and practice towards ischemic bowel disease
Authors: Ying Mei , Li Yan-Ju , Chen Yan , Fu Ming-Yan , Zhang Ge 
Published: January 2025

BACKGROUND
Ischemic bowel disease (IBD) is a critical condition caused by reduced blood flow to the intestines, leading to tissue damage and potentially severe complications. Early recognition and timely management are essential for improving patient outcomes and reducing morbidity and mortality associated with IBD.
AIM
To evaluate the knowledge, attitude and practice (KAP) of healthcare professionals regarding IBD.
METHODS
This cross-sectional study was conducted among healthcare professionals in China from November 2023 to December 2023 using a self-designed questionnaire.
RESULTS
A total of 315 valid questionnaires were analyzed, with 215 participants (68.25%) being female. The mean KAP scores were 17.55 ± 5.35 (range: 0-24), 27.65 ± 2.77 (range: 8-40), and 18.88 ± 4.23 (range: 6-30), respectively. Multivariate linear regression analysis revealed the following factors to be independently associated with knowledge: Age 26-35 years (β = 2.80, 95%CI: 0.31-5.30, P = 0.028), professional title (β = 2.66, 95%CI: 0.91-4.41, P = 0.003), position (β = -3.78, 95%CI: -5.45 to -2.11, P < 0.001), participation in IBD-related training (β = 3.45, 95%CI: 2.39-4.51, P < 0.001), and admission of more than five IBD cases in the past month (β = 3.25, 95%CI: 1.58-4.92, P < 0.001). Attitude was independently associated with knowledge (β = 0.20, 95%CI: 0.15-0.26, P < 0.001) and being a nurse or nursing supervisor (β = -1.30, 95%CI: -2.16 to -0.40, P = 0.003). Practice was independently associated with knowledge (β = 0.20, 95%CI: 0.10-0.30, P < 0.001) and attitude (β = 0.24, 95%CI: 0.06-0.42, P = 0.007). Structural equation modeling demonstrated direct effects of knowledge on attitude (β = 0.24, P < 0.001) and practice (β = 0.26, P < 0.001), as well as of attitude on practice (β = 0.22, P = 0.012).
CONCLUSION
Healthcare professionals demonstrated adequate knowledge but moderate attitude and inactive practice regarding IBD. Addressing the gaps in attitude and practice through targeted training programs and interventions is essential for improving patient care and outcomes.
Efficacy-cost analysis of endoscopic mucosal resection and cold snare polypectomy: A propensity score matching analysis
Authors: Zhang Shi-Yi , Wang Ying-Chun , Liu Lei-Lei , Wang Zhi-Heng , Guan Xue-Mei 
Published: January 2025

BACKGROUND
Although substantial evidence supports the advantages of cold snare polypectomy (CSP) in terms of polypectomy efficacy and reduced postoperative adverse events, few studies have examined the cost differences between CSP and traditional endoscopic mucosal resection (EMR) for the treatment of intestinal polyps.
AIM
To compare the efficacy-cost of EMR and CSP in the treatment of intestinal polyps.
METHODS
A total of 100 patients with intestinal polyps were included in the retrospective data of our hospital from April 2022 to May 2023. According to the treatment methods, they were divided into EMR (n = 46) group and CSP (n = 54) group. The baseline data of the two groups were balanced by 1:1 propensity score matching (PSM), and the cost-effectiveness analysis was performed on the two groups after matching. The recurrence rate of the two groups of patients was followed up for 1 year, and they were divided into recurrence group and non-recurrence group according to whether they recurred. Multivariate logistic regression analysis was used to screen out the influencing factors affecting the recurrence of intestinal polyps after endoscopic resection.
RESULTS
Significant disparities were observed in the number of polyps and smoking background between the two groups before PSM (P < 0.05). Following PSM, the number of polyps and smoking history were well balanced between the EMR and CSP groups. The direct cost incurred by the CSP group was markedly higher than that incurred by the EMR group. Concurrently, the cost-effectiveness ratio in the CSP group was substantially reduced when juxtaposed with that in the EMR group (P < 0.05). Upon completion of the 1-year follow-up, the rate of recurrence after endoscopic intestinal polypectomy was 38.00%. Multivariate methods revealed that age ≥ 60 years, male sex, number of polyps ≥ 3, and pathological type of adenoma were risk factors for recurrence after endoscopic intestinal polypectomy (all P < 0.05).
CONCLUSION
CSP was more cost-effective for the treatment of intestinal polyps. An age ≥ 60 years, male sex, having a number of polyps ≥ 3, and pathological type of adenoma are independent influencing factors for recurrence.
Predicting post-hepatectomy liver failure using a nomogram based on portal vein width, inflammatory indices, and the albumin-bilirubin score
Authors: Sun Ke , Li Jiang-Bin , Chen Ya-Feng , Zhai Zhong-Jie , Chen Lang , Dong Rui 
Published: January 2025

BACKGROUND
Post-hepatectomy liver failure (PHLF) after liver resection is one of the main complications causing postoperative death in patients with hepatocellular carcinoma (HCC). It is crucial to help clinicians identify potential high-risk PHLF patients as early as possible through preoperative evaluation.
AIM
To identify risk factors for PHLF and develop a prediction model.
METHODS
This study included 248 patients with HCC at The Second Affiliated Hospital of Air Force Medical University between January 2014 and December 2023; these patients were divided into a training group (n = 164) and a validation group (n = 84) via random sampling. The independent variables for the occurrence of PHLF were identified by univariate and multivariate analyses and visualized as nomograms. Ultimately, comparisons were made with traditional models via receiver operating characteristic (ROC) curves, calibration curves, and decision curve analysis (DCA).
RESULTS
In this study, portal vein width [odds ratio (OR) = 1.603, 95%CI: 1.288-1.994, P ≤ 0.001], the preoperative neutrophil-to-lymphocyte ratio (NLR) (OR = 1.495, 95%CI: 1.126-1.984, P = 0.005), and the albumin-bilirubin (ALBI) score (OR = 8.868, 95%CI: 2.144-36.678, P = 0.003) were independent risk factors for PHLF. A nomogram prediction model was developed using these factors. ROC and DCA analyses revealed that the predictive efficacy and clinical value of this model were better than those of traditional models.
CONCLUSION
A new Nomogram model for predicting PHLF in HCC patients was successfully established based on portal vein width, the NLR, and the ALBI score, which outperforms the traditional model.
Prognostic value of combined detection of alpha-fetoprotein, plasma prothrombin activity, and serum prealbumin in acute-on-chronic liver failure
Authors: Duan Rui-Xian , Liu Ling , Wang Yan , Wu Wen-Ming 
Published: January 2025

BACKGROUND
Acute-on-chronic liver failure (ACLF) is a liver disease based on chronic liver disease, which is significantly influenced by clinical treatment regimen and disease status, and despite the existence of multiple prognostic assessment indicators for ACLF, the overall sensitivity and accuracy are relatively low.
AIM
To investigate the prognostic value of the combined detection of alpha-fetoprotein (AFP), plasma prothrombin activity (PTA), and serum prealbumin (PA) in ACLF.
METHODS
This retrospective study included 87 patients with ACLF admitted from February 2021 to February 2023 and categorized them into the survival (n = 47) and death (n = 40) groups according to their clinical outcomes 3 months posttreatment. All the participants underwent AFP, PTA, and PA level measurements upon admission. Baseline data, as well as AFP, PTA, and PA levels, were comparatively analyzed. Pearson correlation coefficients were utilized to analyze the correlations of AFP, PTA, and PA with different survival outcomes in patients with ACLF. Receiver operating characteristic (ROC) curves and areas under the curves were used to evaluate the predictive value of AFP, PTA, and PA for ACLF prognosis.
RESULTS
AFP, PTA, and PA levels were markedly decreased in the death group than in the survival group (P < 0.05). Pearson analysis indicated a positive association of the AFP, PTA, and PA levels with the survival of patients with ACLF (P < 0.05). ROC curve analysis determined the sensitivity and specificity of the combined diagnosis at 91.24% and 100.00%, respectively, both of which were notably increased compared to the single-index diagnosis. The ROC of their combined diagnosis was 0.989, significantly surpassing 0.907, 0.849, and 0.853 of AFP, PTA, and PA, respectively. No statistically significant variance was determined in the sensitivity and specificity of the combined diagnosis vs the single detection (P > 0.05).
CONCLUSION
The combined detection of AFP, PTA, and PA levels demonstrates favorable diagnostic value for the short-term prognosis of patients with ACLF, featuring high sensitivity and specificity.