bluuebunny/crossref_metadata_2025_split huggingface dataset:
Biopolymer-based emulsions for the stabilization of Trichoderma atrobrunneum conidia for biological control
Authors: Martínez Yolanda , Heeb Markus , Kalač Tine , Gholam Zennat , Schwarze Francis W.M.R. , Nyström Gustav , De France Kevin 
Published: January 2023

Abstract
Trichoderma spp. are ubiquitous soil-borne fungi that are widely used in biological control to promote and regulate healthy plant growth, as well as protect against plant pathogens. However, as with many biological materials, the relative instability of Trichoderma propagules limits its practical use in industrial applications. Therefore, there has been significant research interest in developing novel formulations with various carrier substances that are compatible with these fungal propagules and can enhance the shelf-life and overall efficacy of the Trichoderma. To this end, herein, we investigate the use of a variety of biopolymers and nanoparticles for the stabilization of Trichoderma atrobrunneum T720 conidia for biological control. The best-performing agents—agar and cellulose nanocrystals (CNC)—were then used in the preparation of oil-in-water emulsions to encapsulate conidia of T720. Emulsion properties including oil type, oil:water ratio, and biopolymer/particle concentration were investigated with respect to emulsion stability, droplet size, and viability of T720 conidia over time. Overall, agar-based formulations yielded highly stable emulsions with small droplet sizes, showing no evidence of drastic creaming, or phase separation after 1 month of storage. Moreover, agar-based formulations were able to maintain ~ 100% conidial viability of T720 after 3 months of storage, and over 70% viability after 6 months. We anticipate that the results demonstrated herein will lead to a new generation of significantly improved formulations for practical biological control applications.

Graphical abstract

Key points
• Various biopolymers were evaluated for improving the stability of Trichoderma conidia
• Oil in water emulsions was prepared using cellulose nanocrystals and agar as interface stabilizers
• Agar-based emulsions showed ~ 100% viability for encapsulated conidia after 3 months of storage
Radiotherapy enhances CXCR3highCD8+ T cell activation through inducing IFNγ-mediated CXCL10 and ICAM-1 expression in lung cancer cells
Authors: Wang Chih-Liang , Ho Ai-Sheng , Chang Chun-Chao , Sie Zong-Lin , Peng Cheng-Liang , Chang Jungshan , Cheng Chun-Chia 
Published: January 2023

AbstractRadiotherapy (RT) not only damages tumors but also induces interferon (IFN) expression in tumors. IFNs mediate PD-L1 to exhaust CD8+ T cells, but which also directly impact tumor cells and potentially activate anti-tumor immune surveillance. Little is known about the contradictory mechanism of IFNs in regulating CD8+ T-mediated anti-tumor activity in lung cancer. This study found that RT induced IFNs and CXCL9/10 expression in the RT-treated lung cancer cells. Specifically, RT- and IFNγ-pretreated A549 significantly activated CD8+ T cells, resulting in significant inhibition of A549 colony formation. RNAseq and consequent qPCR results revealed that IFNγ induced PD-L1, CXCL10, and ICAM-1, whereas PD-L1 knockdown activated CD8+ T cells, but ICAM-1 knockdown diminished CD8+ T cell activation. We further demonstrated that CXCR3 and CXCL10 decreased in the CD8+ T cells and nonCD8+ PBMCs, respectively, in the patients with lung cancer that expressed lower reactivation as co-cultured with A549 cells. In addition, inhibitors targeting CXCR3 and LFA-1 in CD8+ T cells significantly diminished CD8+ T cell activation and splenocytes-mediated anti-LL/2shPdl1. In conclusion, we validated that RT suppressed lung cancer and overexpress PD-L1, CXCL10, and ICAM-1, which exhibited different roles in regulating CD8+ T cell activity. We propose that CXCR3highCD8+ T cells stimulated by CXCL10 exhibit anti-tumor immunity, possibly by enhancing T cells-tumor cells adhesion through CXCL10/CXCR3-activated LFA-1-ICAM-1 interaction, but CXCR3lowCD8+ T cells with low CXCL10 in patients with lung cancer were exhausted by PD-L1 dominantly. Therefore, RT potentially activates CD8+ T cells by inducing IFNs-mediated CXCL10 and ICAM-1 expression in tumors to enhance CD8+ T-tumor adhesion and recognition. This study clarified the possible mechanisms of RT and IFNs in regulating CD8+ T cell activation in lung cancer.
Sarcopenia indicate poor survival in patients undergoing transarterial chemoembolization (TACE) for hepatic malignancies
Authors: Loosen Sven H. , Jördens Markus S. , Schoon Berenike , Antoch Gerald , Luedde Tom , Minko Peter , Loberg Christina , Roderburg Christoph 
Published: January 2023

Abstract
Background
Patient selection for transarterial chemoembolization (TACE) has remained challenging. Currently used markers mainly reflect liver function and turned out as less reliable in larger clinical trials. The patients´ body composition has been linked with patient outcome in different cancers. Now, we analyzed the function of different parameters of the patient’s body composition as prognostic and/ or predictive parameters in patients that received TACE.

Methods
CT scans were used to assess five parameters of the individual body composition (skeletal muscle index (SMI), median muscular attenuation (MMA), bone mineral density (BMD) as well as the visceral and subcutaneous fat area) in 89 patients undergoing TACE. Results were correlated with tumor response to TACE and outcome of patients.

Results
SMI and visceral fat area were significantly higher in male patients and among patients undergoing TACE for HCC compared to patients with liver metastases. While all parameters of the body composition did not predict response to TACE, patients with an SMI below the ideal cutoff value of 37.76 cm2/m2 had a significantly reduced long-term outcome with a median overall survival of 404 days compared to 1321 days for patients with a high SMI. Moreover, the pre-interventional SMI turned out as an independent prognostic factor in a multivariate Cox regression model including clinicopathological parameters and laboratory markers of organ dysfunction and systemic inflammation (HR: 0.899, 95% CI 0.827–0.979, p = 0.014).

Conclusion
The pre-interventional SMI represents an independent prognostic factor for overall survival following TACE. Assessment of the individual body composition using routine CT scan might help to identify the ideal patients for TACE.
Pupal productivity of larval habitats of Aedes aegypti in Msambweni, Kwale County, Kenya
Authors: Mwakutwaa Alawih S. , Ngugi Harun N. , Ndenga Bryson A. , Krystosik Amy , Ngari Moses , Abubakar Laila U. , Yonge Shadrack , Kitron Uriel , LaBeaud A. Desiree , Mutuku Francis M. 
Published: January 2023

AbstractAedes aegypti is an important vector of several arboviruses including dengue and chikungunya viruses. Accurate identification of larval habitats of Ae. aegypti is considered an essential step in targeted control. This study determined Ae. aegypti productivity in selected larval habitats in Msambweni, Kwale County, Kenya. Three sequential larval habitat surveys were conducted. The first survey was habitat census (baseline) through which 83 representative larval habitats were identified and selected. The second and third surveys involved estimating daily productivity of the 83 selected larval habitats for 30 consecutive days during a wet and a dry season, respectively. Of 664 larval habitats examined at baseline, 144 larval habitats (21.7%) were found to be infested with Ae. aegypti larvae. At baseline, majority (71%) of the pupae were collected from two (2/6) larval habitat types, tires and pots. Multivariate analysis identified habitat type and the habitat being movable as the predictors for pupal abundance. During the 30-day daily pupal production surveys, only a few of the habitats harbored pupae persistently. Pupae were found in 28% and 12% of the larval habitats during the wet and dry seasons, respectively. In the wet season, drums, tires, and pots were identified as the key habitat types accounting for 85% of all pupae sampled. Three habitats (all drums) accounted for 80% of all the pupae collected in the dry season. Predictors for pupal productivity in the wet season were habitat type, place (whether the habitat is located at the back or front of the house), habitat purpose (use of the water in the habitat), and source of water. Although the multivariate model for habitat type did not converge, habitat type and habitat size were the only significant predictors during the dry season. Drums, pots, and tires were sources of more than 85% of Ae. aegypti pupae, reinforcing the “key container concept.” Targeting these three types of habitats makes epidemiological sense, especially during the dry season.
Random vibration of hysteretic systems under Poisson white noise excitations
Authors: Chen Lincong , Yuan Zi , Qian Jiamin , Sun J. Q. 
Published: January 2023

AbstractHysteresis widely exists in civil structures, and dissipates the mechanical energy of systems. Research on the random vibration of hysteretic systems, however, is still insufficient, particularly when the excitation is non-Gaussian. In this paper, the radial basis function (RBF) neural network (RBF-NN) method is adopted as a numerical method to investigate the random vibration of the Bouc-Wen hysteretic system under the Poisson white noise excitations. The solution to the reduced generalized Fokker-Planck-Kolmogorov (GFPK) equation is expressed in terms of the RBF-NNs with the Gaussian activation functions, whose weights are determined by minimizing the loss function of the reduced GFPK equation residual and constraint associated with the normalization condition. A steel fiber reinforced ceramsite concrete (SFRCC) column loaded by the Poisson white noise is studied as an example to illustrate the solution process. The effects of several important parameters of both the system and the excitation on the stochastic response are evaluated, and the obtained results are compared with those obtained by the Monte Carlo simulations (MCSs). The numerical results show that the RBF-NN method can accurately predict the stationary response with a considerable high computational efficiency.
The Practice and Feasibility of Screening, Treatment, and Referral for Gaming Problems in Gambling, Alcohol and Other Drugs, and Youth Services
Authors: Park Jennifer J. , King Daniel L. , Wilkinson-Meyers Laura , Rodda Simone N. 
Published: January 2023

AbstractScreening, brief intervention, and referral to treatment (SBIRT) is a comprehensive approach to identifying people at risk of addiction, but its feasibility for gaming disorder is unknown. This study surveyed 88 clinicians from gambling, alcohol and other drugs, and youth services in New Zealand. Results indicated that the most frequent GD screening method was an unstructured interview (61%), but 74% stated they would use a standardized tool if available. Responsivity to the detection of GD was an immediate intervention (84%), and rates of referral were low (28%). Around 50% of clinicians indicated high confidence in administering motivational approaches and relapse prevention. There was strong support for screening training (85%), treatment guidelines (88%), self-help materials (92%), and access to internet-delivered CBT that could be used in conjunction with other treatment (84%). Clinicians appear motivated and willing to implement SBIRT for GD but report lacking necessary training and resources, including access to screening tools and treatment guidelines.
Advances in HIV Research Using Mass Cytometry
Authors: George Ashley F. , Roan Nadia R. 
Published: January 2023

Abstract

Purpose of Review
This review describes how advances in CyTOF and high-dimensional analysis methods have furthered our understanding of HIV transmission, pathogenesis, persistence, and immunity.

Recent Findings
CyTOF has generated important insight on several aspects of HIV biology: (1) the differences between cells permissive to productive vs. latent HIV infection, and the HIV-induced remodeling of infected cells; (2) factors that contribute to the persistence of the long-term HIV reservoir, in both blood and tissues; and (3) the impact of HIV on the immune system, in the context of both uncontrolled and controlled infection.

Summary
CyTOF and high-dimensional analysis tools have enabled in-depth assessment of specific host antigens remodeled by HIV, and have revealed insights into the features of HIV-infected cells enabling them to survive and persist, and of the immune cells that can respond to and potentially control HIV replication. CyTOF and other related high-dimensional phenotyping approaches remain powerful tools for translational research, and applied HIV to cohort studies can inform on mechanisms of HIV pathogenesis and persistence, and potentially identify biomarkers for viral eradication or control.
Anti-tumor effect of Wasabi component, 6-(methylsulfinyl) hexyl isothiocyanate, against endometrial carcinoma cells
Authors: Ono Motoki , Miyamoto Tsutomu , Fuseya Chiho , Asaka Ryoichi , Ando Hirofumi , Tanaka Yasuhiro , Shinagawa Manaka , Yokokawa Yusuke , Takeuchi Hodaka , Horiuchi Akiko , Shiozawa Tanri 
Published: January 2023

Abstract
Purpose
Wasabi is a traditional plant seasoning with an anti-septic function. Recent studies revealed several functions of Wasabi, such as anti-inflammation; however, the anti-tumor effect against endometrial carcinoma (EMC) cells has not been examined. In the present study, we investigated the anti-tumor effect of 6-(methylsulfinyl) hexyl isothiocyanate (6-MITC), a major chemical compound of Wasabi, against various EMC cell lines in vitro and in vivo.

Methods
The effect of 6-MITC on cell viability was measured by the WST-1 assay in EMC and HUVEC cells. The impact of 6-MITC oral administration in nude mice was measured to assess the growth of the EMC xenograft and natural killer (NK) cell activity in the spleen.

Results
The addition of 6-MITC suppressed the proliferation of EMC cells (Ishikawa, HEC265, HEC108, KLE, and HEC1B) dose-dependently, but not HUVEC cells. 6-MITC (5 µM) enhanced the cisplatin sensitivity of EMC cells. 6-MITC induced apoptosis in a dose-dependent fashion in EMC cells other than HEC1B cells and was associated with increased expression of cleaved-caspase3 and decreased expression of BCL2. Oral administration of 6-MITC (2 and 4 µmol/kg) to Ishikawa and HEC1B xenografting mice resulted in a reduced tumor volume compared with the control (P < 0.05, 4 µmol/kg). Immunohistochemical staining of resected tumors revealed increased expression of Ki-67 and reduced cleaved-caspase3. Furthermore, 6-MITC treatment enhanced NK cell activity, especially when administered before tumor xenografting.

Conclusion
These results indicate that 6-MITC has a marked anti-tumor effect against EMC cells and a novel effect to enhance NK cell activity. These effects suggest the therapeutic potential of 6-MITC.
How to Study Religion? Notes on Research Methodology in the Context of Latin American Religions
Authors: Rodrigues Donizete 
Published: January 2023

AbstractThe aim of this paper is to discuss on the main methodological procedures used in Anthropology and Sociology and applied in studies of Latin American religions, particularly in the context of diasporic Brazilian Protestantism-Pentecostalism. After introduce the two principal categories (quantitative and qualitative) – which include various types of procedures, such as the case study, interdisciplinary, historical, comparative and cross-cultural—and based on ethnographic experiences of the author in America, Europe and Asia, examine the world scale and ‘glocal’ multi-situated ethnography and the traditional localized participant-observation, including the ‘outsider-insider dichotomy’ and Asia, the article examines. However, today, with new digital technologies and the broad cultural and religious manifestations in the Internet, the researcher can complement the search for information (ethnographic data)—and accompany of the daily life of the group, of the community under study—using the Internet, the various social networks, namely, Facebook, Twitter, and WhatsApp. The principal contribution of this work is to present the specificities of the ethnographic field in the studies of religious movements, evangelical churches, in particular, where issues and problems posed to researchers requiring appropriate ethical and methodological procedures for overcoming them.
Geriatric care for surgical patients: results and reflections from a cross-sectional survey in acute Belgian hospitals
Authors: Fagard Katleen , Deschodt Mieke , Geyskens Lisa , Willems Sarah , Boland Benoît , Wolthuis Albert , Flamaing Johan 
Published: January 2023

Abstract
Purpose
To explore geriatric care for surgical patients in Belgian hospitals and geriatricians’ reflections on current practice.

Methods
A web-based survey was developed based on literature review and local expertise, and was pretested with 4 participants. In June 2021, the 27-question survey was sent to 91 heads of geriatrics departments. Descriptive statistics and thematic analysis were performed.

Results
Fifty-four surveys were completed, corresponding to a response rate of 59%. Preoperative geriatric risk screening is performed in 25 hospitals and systematically followed by geriatric assessment in 17 hospitals. During the perioperative hospitalisation, 91% of geriatric teams provide non-medical and 82% provide medical advice. To a lesser extent, they provide geriatric protocols, geriatric education and training, and attend multidisciplinary team meetings. Overall, time allocation of geriatric teams goes mainly to postoperative evaluations and interventions, rather than to preoperative assessment and care planning. Most surgical patients are hospitalised on surgical wards, with reactive (73%) or proactive (46%) geriatric consultation. In 36 hospitals, surgical patients are also admitted on geriatric wards, predominantly orthopaedic/trauma, abdominal and vascular surgery. Ninety-eight per cent of geriatricians feel that more geriatric input for surgical patients is needed. The most common reported barriers to further implement geriatric-surgical services are shortage of geriatricians and geriatric nurses, and unadjusted legislation and financing.

Conclusion
Geriatric care for surgical patients in Belgian hospitals is mainly reactive, although geriatricians favour more proactive services. The main opportunities and challenges for improvement are to resolve staff shortages in the geriatric work field and to update legislation and financing.
New Explicit and Approximate Solutions of the Newton-Schrödinger System
Authors: Bougoffa Lazhar , Khanfer Ammar , Bougouffa Smail 
Published: January 2023

AbstractIn this paper, we consider the Newton-Schrödinger system $$\begin{aligned} \left\{ \begin{array}{rcl} \nabla ^{2} \Psi & =& \left( \gamma \Phi +a(x)\right) \Psi ,\\ \nabla ^{2} \Phi & =& \mid \Psi \mid ^{2}, \end{array} \right. \end{aligned}$$

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a
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2

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which arises in certain quantum transport and chemistry problems. Explicit analytic solutions, which contain an auxiliary parameter, are obtained. An existence and uniqueness theorem to this nonlinear system subject to the boundary conditions is proved. Also, we introduce approximate solutions to the modified Newton-Schrödinger system in the case of spherically-symmetric stationary and time-independence by the Adomian decomposition method.
The Brussels Effect, European Regulatory Power and Political Capital: Evidence for Mutually Reinforcing Internal and External Dimensions of the Brussels Effect from the European Digital Policy Debate
Authors: Bendiek Annegret , Stuerzer Isabella 
Published: January 2023

AbstractAnu Bradford has described the European Union’s ability to externalise its norms and standards as the so-called Brussels effect. We apply the Brussels effect to select issues discussed via the EU-US Trade and Technology Council (TTC) and show that its capacity to project power stems not only from the EU’s market size but also from its domestic decision-making structure. The political capital accumulated in the EU’s consensus-based and inclusive deliberations functions as an effective instrument for motivating other states to adopt European regulations, as legislative acts resulting from European inter-institutional and multi-level policy-making hold high standards of legal certainty and signal European strategic goals and political commitments credibly. Knowing that European consensus is an important condition for externalisation, Brussels can facilitate consensus by calling for internal compromise in order to be able to take the European compromise to the international stage. Thus, the internal and external dimensions of the Brussels effect are mutually reinforcing. This twofold appearance demarcates it from the California effect and the Beijing effect.
Mapping Multi-Factor-Mediated Chromatin Interactions to Assess Dysregulation of Lung Cancer-Related Genes
Authors: Zhang Yan , Zhang Jingwen , Zhang Wei , Wang Mohan , Wang Shuangqi , Xu Yao , Zhao Lun , Li Xingwang , Li Guoliang 
Published: January 2023

Abstract
Studies on the lung cancer genome are indispensable for developing a cure for lung cancer. Whole-genome resequencing, genome-wide association studies, and transcriptome sequencing have greatly improved our understanding of the cancer genome. However, dysregulation of long-range chromatin interactions in lung cancer remains poorly described. To better understand the three-dimensional (3D) genomic interaction features of the lung cancer genome, we used the A549 cell line as a model system and generated high-resolution chromatin interactions associated with RNA polymerase II (RNAPII), CCCTC-binding factor (CTCF), enhancer of zeste homolog 2 (EZH2), and histone 3 lysine 27 trimethylation (H3K27me3) using long-read chromatin interaction analysis by paired-end tag sequencing (ChIA-PET). Analysis showed that EZH2/H3K27me3-mediated interactions further repressed target genes, either through loops or domains, and their distributions along the genome were distinct from and complementary to those associated with RNAPII. Cancer-related genes were highly enriched with chromatin interactions, and chromatin interactions specific to the A549 cell line were associated with oncogenes and tumor suppressor genes, such as additional repressive interactions on FOXO4 and promoter–promoter interactions between NF1 and RNF135. Knockout of an anchor associated with chromatin interactions reversed the dysregulation of cancer-related genes, suggesting that chromatin interactions are essential for proper expression of lung cancer-related genes. These findings demonstrate the 3D landscape and gene regulatory relationships of the lung cancer genome.