bluuebunny/crossref_metadata_2025_split huggingface dataset:
Chemical Zymogens: Specificity and Steroidal Control of Reactivation
Authors: Casanovas Montasell Mireia , Baumann Anna , Zelikin Alexander N. 
Published: April 2023

AbstractActivating and masking enzymatic activity on demand is of the highest importance in nature. It is achieved by chemical interconversion of enzymes and the corresponding zymogens through, for example, proteolytic processing or reversible phosphorylation, and affords on‐demand activation of enzymes, controlled in space and/or time. In stark contrast, examples of chemical zymogens are very few, and in most cases these are based on disulfide chemistry, which is largely indiscriminate as to the nature of the activating thiol. In this work, we address an outstanding challenge of specificity of reactivation of chemical zymogens. We achieve this through engineering affinity between the chemical zymogen and the activator. Additional, higher‐level control over zymogen reactivation is installed in a nature‐mimicking approach using steroidal hormones. Taken together, the results of this study take a step towards establishing the specificity of reactivation of synthetic, chemical zymogens. We anticipate that the results of this study will contribute significantly to the development of chemical zymogens as tools for diverse use in chemical biology and biotechnology.
Left atrial appendage occlusion using hydrogel coils in a child
Authors: Mohammad Nijres Bassel , Reinking Benjamin , Aldoss Osamah 
Published: April 2023

AbstractLeft atrial appendage occlusion in young children has not been reported before. Herein, we describe a successful occlusion using hydrogel coils in a toddler. The decision to occlude the appendage was made to mitigate the potential risk of systemic thromboembolism, given the child's unusual anatomy.
Disseminated ectopic pregnancy after salpingotomy in a 30‐year‐old patient
Authors: Matlac D. M. , Ribbat‐Idel J. , Stroschein K. , Perner S. , Rody A. , Offermann D. 
Published: April 2023

AbstractWe present a case of a 30‐year old patient who devoloped a disseminated abdominal pregnancy after receiving a salpingotomy due to a prior tubal pregnancy.
Think zinc: Transient nutritional deficiency related to novel maternal SLC30A2 mutation potentially precipitated by antenatal proton pump inhibitor exposure
Authors: Porter Emma , Molloy Oonagh , Murphy Michelle , O'Connor Cathal 
Published: April 2023

AbstractA second‐born breastfed infant presented with zinc deficiency. His mother had a novel heterozygous mutation in SLC30A2. A previous baby did not have zinc deficiency but the mother had taken a proton pump inhibitor (PPI) during the second pregnancy. Antenatal PPI exposure may plausibly contribute to transient infantile zinc deficiency.
Reversible Pressure‐Magnetic Modulation in a Tetrathiafulvalene‐Based Dyad Piezochromic Dysprosium Single‐Molecule Magnet**
Authors: Pointillart Fabrice , Flores Gonzalez Jessica , Douib Haiet , Montigaud Vincent , McMonagle Charles J. , Le Guennic Boris , Cador Olivier , Pinkowicz Dawid , Probert Michael R. 
Published: April 2023

AbstractThe extreme sensitivity of trivalent lanthanide ions to crystal field variations led to the emergence of single‐molecule magnetic switching under various stimuli. The use of pressure as an external stimulus instead of classic light irradiation, oxidation or any chemical reactions allows a fine tuning of the magnetic modulation. Here the well‐known pure isotopically enriched [162Dy(tta)3(L)]⋅C6H14 (162Dy) Single‐Molecule Magnet (SMM) (tta−=2‐2‐thenoyltrifluoroacetonate and L=4,5‐bis(propylthio)‐tetrathiafulvalene‐2‐(2‐pyridyl)benzimidazole‐methyl‐2‐pyridine) was experimentally investigated by single‐crystal diffraction and squid magnetometry under high applied pressures. Both reversible piezochromic properties and pressure modulation of the slow magnetic relaxation behavior were demonstrated and supported by ab initio calculations. The magnetic study of the diluted sample [162Dy0.05Y0.95(tta)3(L)]⋅C6H14 (162Dy@Y) indicated that variations in the electronic structure have mainly intermolecular origin with weak intramolecular contribution. Quantitative magnetic interpretation concludes to a deterioration of the Orbach process for the benefit of both Raman and QTM mechanisms under applied pressure.
Palladium Catalyzed, Annulative Multiple C‐H Bond Activations of BODIPYs to Access π‐Extended Polycyclic Heteroaromatic Molecules with Deep Red Emissions andSelf‐aggregation Behaviors
Authors: Miao Wei , Gan Rui , Liu Wanxiao , Guo Xing , Wu Qinghua , Wei Ying , Yu Changjiang , Hao Erhong , Jiao Lijuan 
Published: April 2023

AbstractAromatic ring fusion on BODIPY core can effectively tune its electronic property, and red‐shift its absorption and emission wavelength. In this work, we report that a one‐pot Pd(II) catalyzed multiple C−H activation to access acenaphtho[b]‐fused BODIPYs though the reaction ofα,β‐unsubstituted‐BODIPYs and 1,8‐dibromonaphthalenes. These newly synthesized acenaphtho[b]‐fused BODIPYs revealed intensified deep red absorptions (639–669 nm) and emissions (643–683 nm), with high fluorescence quantum yields (0.53–0.84) in dichloromethane. Notably, these acenaphtho[b]‐fused BODIPYs exhibited well‐defined self‐aggregation behavior in water/THF mixture, and for instance, the absorption of3 awas red‐shifted by 53 nm to 693 nm after forming aggregates.
Flow Synthesis of Gigantic Porphyrinic Cages: Facile Synthesis of P12L24 and Discovery of Kinetic Product P9L18
Authors: Lee Hochan , Joo Jeong‐Un , Dhamija Avinash , Gunnam Anilkumar , Koo Jaehyoung , Giri Pritam , Ho Ko Young , Hwang In‐Chul , Kim Dong‐Pyo , Kim Kimoon 
Published: April 2023

AbstractA continuous flow methodology for the facile and high‐yielding synthesis of the porphyrin‐based self‐assembled organic cage, P12L24 is reported, along with the serendipitous discovery of a kinetic product, P9L18 cage, which has been characterized by MALDI‐TOF MS, NMR, and AFM analysis. A theoretical study suggests a tricapped trigonal prismatic geometry for P9L18. Unlike P12L24, P9L18 is unstable and readily decomposes into monomers and small oligomers. While the batch synthesis produces only the thermodynamic product P12L24, the continuous flow process generates not only the thermodynamic product but also kinetic products, such as P9L18, illustrating the advantages of the continuous flow process for the synthesis of self‐assembled cages and the exploration of new non‐equilibrium assemblies.
A Brick‐Wall Topological 2D Covalent Organic Framework Constructed from an H‐Shaped “Two‐in‐One” Monomer
Authors: Bao Pengli , Yang Zongfan , Yan Xiaoli , Guo Linshuo , Su Xi , Zhang Guang , Yuan Daqiang , Chen Long 
Published: April 2023

AbstractAs an emerging class of porous crystalline material, covalent organic frameworks (COFs) have received considerable research interests in terms of exploring new architectures and functions. Herein, we developed an unprecedented “H‐shaped” monomer, upon self‐polycondensation, which facilely produced a benzoimidazole‐based COF (H‐BIm‐COF) with a rarely reported brick‐wall topology. H‐BIm‐COF displayed high crystallinity, nano‐sized porosity, and high thermal and chemical stabilities. Interestingly, H‐BIm‐COF based membranes showed selective permeability towards different solvents, which related to the size and polarity of the guest molecule. Additionally, initial study suggested the COF displayed excellent rejection efficiency towards ionic dyes, for example chromium black T (99.7 %) and rhodamine B (97.3 %). This work provides insights into developing new topological COFs by designing monomers with new configurations.
ProMetaS – A Metadata Schema for Process Engineering and Industry
Authors: Sherpa Lincoln , Müller-Pfefferkorn Ralph , Tolksdorf Gregor , Khaydarov Valentin , Wiedau Michael , Urbas Leon 
Published: April 2023

AbstractData collection in process industry yields a variety of data types. To maintain the knowledge about the data and to enable finding and reusing them (FAIR data), a common description with metadata is necessary. In the project KEEN, ProMetaS – the Process Engineering/Industry Metadata Schema – was developed. It defines various metadata categories adhering to available metadata standards. In this article, ProMetaS, its implementation into the KEEN data platform, and its application in two use cases is described.
Clinical outcomes of ultrasound‐guided radiofrequency ablation for solitary T1N0M0 papillary thyroid carcinoma: A retrospective study with more than 5 years of follow‐up
Authors: Yan Lin , Li Yingying , Li Xin Yang , Xiao Jing , Tang Jie , Luo Yukun 
Published: April 2023

AbstractBackgroundUltrasound‐guided radiofrequency ablation (RFA) has been used in patients with papillary thyroid carcinoma (PTC) who refuse surgery or active surveillance. However, the long‐term outcomes are still limited. This study aimed to evaluate the clinical outcomes of RFA for solitary T1N0M0 PTC in a large cohort over a more than 5‐year follow‐up period.MethodsThis retrospective study included 358 patients with solitary T1N0M0 PTC who were treated with RFA and followed for at least 5 years. The bipolar RFA procedure was performed using hydrodissection technique, transisthmic approach, and moving‐shot technique. The primary outcomes were disease progression, including lymph node metastasis (LNM), recurrent tumor, persistent tumor, and distant metastasis. The secondary outcomes were volume reduction rate, complete disappearance rate, complications, and delayed surgery.ResultsDuring a mean follow‐up period of 75.5 ± 9.7 months, the overall disease progression was 5.0%. The incidence of LNM, recurrent tumor, and persistent tumor was 1.4%, 3.1%, and 0.6%, respectively. There were no significant differences in the disease progression (5.0% vs. 5.5%, p = 1.000), LNM (1.3% vs. 1.8%, p = .568), recurrent tumor (3.3% vs. 1.8%, p = .872), persistent tumors (0.3% vs. 1.8%, p = .284), and 5‐year recurrence‐free survival rates (95.4% vs. 96.4%, p = .785) in the T1a and T1b groups. Volume reduction rate was 100.0 ± 0.3%, with 96.9% of tumors disappearing. No complications occurred. No patients underwent delayed surgery because of anxiety.ConclusionsRFA is an effective and safe alternative for patients with T1N0M0 PTC and can offer a minimally invasive curative option for patients who refuse surgery or active surveillance.Plain Language Summary

During a mean follow‐up period of 75.5 ± 9.7 months, the overall papillary thyroid carcinoma disease progression was 5.0%. The volume reduction rate was 100.0 ± 0.3%, with 96.9% of tumors disappearing.
The T1a and T1b groups had similar incidence of disease progression and 5‐year recurrence‐free survival rates. No patients experienced complications or underwent delayed surgery because of anxiety.
Color induction in the restoration of architecture in historic city centers
Authors: Serra Juan , Cabodevilla‐Artieda Ignacio , Torres‐Barchino Ana , Llopis Jorge 
Published: April 2023

AbstractIn the restoration of historic facades, specialists need to tackle the problem of colors applied to the ornamental elements that appear different on a facade than if they were isolated, because the colors of the surrounding surfaces influence them through color induction. This study evaluates the difference between the colors of ornaments observed in isolation, which we call nominal colors, and on different background colors, which we call induced colors, considering the historic color palette of Valencia as studied by the Color Research Group in Architecture of the Universitat Politècnica de València. The study consisted of the evaluation of 108 color pairs painted on physical cardboards, rated by 26 female students (average age 26.5), under natural lighting conditions, and using the Natural Color System (NCS) Color Atlas for the color match. Results indicate that induced colors have lower NCS blackness compared with nominal ones, and slightly higher or no NCS chromaticness shift. Nominal colors with low chromaticness exhibit an important hue induction effect and tend to shift to a hue that is located opposite to that of the background color in the NCS color circle, this effect being larger when the background has a warm color. The induction effect is larger when ornamental elements and background have similar nominal NCS blackness, and different nominal NCS chromaticness,with nominal hue difference no significant in the induction effect. The colors of ornamental elements that entailed greater induction were those with the lowest NCS chromaticness, and in the case of the background color, those with the highest chromaticness.
Carbon‐Based Electrodes for Organic Solar Cells
Authors: Shi Xiaoyu , Chen Shangshang 
Published: April 2023

AbstractOrganic solar cells (OSCs) are a promising low‐cost thin‐film photovoltaic technology while the fabrication of transparent conductive oxide (TCO) and metal electrodes still remains a factor that hinders the scaling‐up and commercialization of OSCs. Carbon‐based materials are regarded as potential alternatives due to their excellent properties, such as low cost, solution processibility, high conductance, and good chemical stability. In this mini‐review, the recent progress of carbon‐based materials such as graphite, carbon nanosheets, graphene, and carbon nanotubes to replace the TCO and metal electrodes of OSCs is surveyed. The preparation methods of different carbon‐based materials are also discussed. Based on current progress, we summarize the outlooks and challenges of carbon‐based electrodes. We anticipate this mini‐review will inspire more research efforts to develop high‐performance and OSC‐matched carbon materials for more efficient and stable carbon‐electrode‐based OSCs.
Fluorescent Protein Production, Purification, and Coupling to Microspheres
Authors: Dramicanin Marija , Lim Kevin , Monard Simon 
Published: April 2023

AbstractFluorescent proteins (FPs) have become an essential tool for biological research. Since the isolation and description of green FP, hundreds of FPs have been discovered and created with various characteristics. The excitation of these proteins ranges from ultraviolet (UV) up to near infrared (NIR). Using conventional cytometry, with each detector assigned to a fluorochrome, great care must be taken when selecting the optimal bandpass filters to minimalize the spectral overlap as the emission spectra of FPs are broad. Full‐spectrum flow cytometers eliminate the need to change optical filters for analyzing FPs, which simplifies instrument setup. In experiments where more than one FP is used, single‐color controls are required. These can be cells expressing each of the proteins separately. In the case of the confetti system, for instance, when four FPs are used, all these proteins will need to be expressed separately so that compensation or spectral unmixing can be performed, and this can be inconvenient and expensive. An appealing alternative is to produce FPs in Escherichia coli, purify them, and covalently couple them to carboxylate polystyrene microspheres. Such microspheres are ready to use and can be stored at 4°C for months or even years without any deterioration in fluorescence. The same procedure can be used to couple antibodies or other proteins to these particles. Here, we describe how to express and purify FPs, how to couple them to microspheres, and how to evaluate the fluorescent properties of the particles. © 2023 The Authors. Current Protocols published by Wiley Periodicals LLC.Basic Protocol 1: Escherichia coli expression and purification of recombinant mPlumBasic Protocol 2: Coupling fluorescent proteins to polystyrene microspheresSupport Protocol 1: Comparing the cell‐bound and bead‐bound fluorescence signaturesSupport Protocol 2: Comparing spectral signatures via the similarity index, complexity matrix, and spillover spread matrix of fluorescent protein–coupled beads
Air filtration mitigates aerosol levels both during and after endoscopy procedures
Authors: Phillips Frank , Crowley Jane , Warburton Samantha , Staniforth Karren , Parra‐Blanco Adolfo , Gordon George S.D. 
Published: April 2023

AbstractObjectivesUpper gastrointestinal endoscopies are aerosol‐generating procedures, increasing the risk of spreading airborne pathogens. We aim to quantify the mitigation of airborne particles via improved ventilation, specifically laminar flow theatres and portable high‐efficiency particulate air (HEPA) filters, during and after upper gastrointestinal endoscopies.MethodsThis observational study included patients undergoing routine upper gastrointestinal endoscopy in a standard endoscopy room with 15–17 air changes per hour, a standard endoscopy room with a portable HEPA filtration unit, and a laminar flow theatre with 300 air changes per hour. A particle counter (diameter range 0.3 μm‐25 μm) took measurements 10 cm from the mouth. Three analyses were performed: whole procedure particle counts, event‐based counts, and air clearance estimation using post‐procedure counts.ResultsCompared to a standard endoscopy room, for whole procedures we observe a 28.5x reduction in particle counts in laminar flow (p < 0.001) but no significant effect of HEPA filtration (p = 0.50). For event analysis, we observe for lateral flow theatres reduction in particles >5 μm for oral extubation (12.2x, p < 0.01), reduction in particles <5 μm for coughing/gagging (6.9x, p < 0.05), and reduction for all sizes in anesthetic throat spray (8.4x, p < 0.01) but no significant effect of HEPA filtration. However, we find that in the fallow period between procedures HEPA filtration reduces particle clearance times by 40%.ConclusionsLaminar flow theatres are highly effective at dispersing aerosols immediately after production and should be considered for high‐risk cases where patients are actively infectious or the supply of personal protective equipment is limited. Portable HEPA filers can safely reduce the fallow time between procedures by 40%.
Maintenance of complete mucosal healing is associated with avoiding restenosis after endoscopic balloon dilation of Crohn's disease‐related small intestinal strictures
Authors: Dashnyam Ulzii , Nagayama Manabu , Yano Tomonori , Sakamoto Hirotsugu , Mieno Makiko , Owada Jun , Oguro Kunihiko , Khurelbaatar Tsevelnorov , Sunada Keijiro , Lefor Alan Kawarai , Yamamoto Hironori 
Published: April 2023

AbstractBackgroundEndoscopic balloon dilation (EBD) is an effective, minimally invasive treatment for Crohn's disease (CD) related intestinal strictures. However, restenosis frequently occurs and requires repetitive EBD or surgical resection. Since previous studies could not evaluate restenosis based on stricture diameter, factors affecting restenosis after EBD were unclear. This study aimed to identify these factors by precisely measuring the diameter of small intestinal strictures in patients with CD.MethodsThis single‐center retrospective study enrolled patients with CD with de novo small intestinal strictures who underwent two double‐balloon enteroscopy sessions (EBD and follow‐up) between January 2016 and October 2021. Clinical and endoscopic data were obtained from electronic medical records. A calibrated small‐caliber‐tip transparent hood was used to precisely measure stricture diameters. Multivariate analysis was performed to identify factors associated with restenosis.ResultsForty‐eight patients (37 male) were analyzed. The total number of strictures detected decreased from 162 to 143. The mean diameter of all strictures and the narrowest stricture in each patient increased significantly from 8.6 to 9.8 mm and from 7.6 to 8.7 mm, respectively. Thirty‐two (67%) patients developed endoscopic restenosis. Multivariate analysis showed that the presence of ulcers at the follow‐up session was a risk factor for restenosis (odds ratio 9.4, p = 0.01). Patients with complete mucosal healing at both sessions (n = 21) showed significant improvement in the narrowest stricture (+1.7 mm, p = 0.001).ConclusionsMaintenance of complete mucosal healing is significantly associated with avoiding restenosis after EBD in CD‐related small intestinal strictures.
First description of injury feigning behavior in Godlewski's buntings
Authors: Liu Xizhu , Zhang Yuhan , Wan Guixia , Luo Haixia , Wang Longwu , Liang Wei 
Published: April 2023

AbstractNest defense behavior helps ground‐nesting birds improve their breeding success. Among such behaviors, injury feigning behavior (IFB) is one of the better ways to attract predators and protect birds' nests. IFB is generally associated with shorebirds in general and plovers in particular, however, through field observation, it has been found this behavior is also exhibited in bunting species when they facing the risk of predation. We attempt to explore the detailed description of this behavior in buntings and the factors that affect this behavior. Based on video‐recordings of the nest defense behavior by the ground‐nesting bird Godlewski's bunting (Emberiza godlewskii) against nest invaders, we explored the characteristic of IFB and differences in nest defense behavior during their brooding seasons. The results showed that female buntings had a distinct IFB: the wings of buntings incited within 60°–90° of the body, ran and called rapidly, pretending to be injured and unable to fly. The nest stage had significant and extremely significant effects on IFB and movement distance (MD), respectively. And IFB was more likely to occur in brooding (34.62%) than incubation period (16.42%). This indicates that there are significant differences in the IFB of buntings at different breeding stages.
Evaluation of community science monitoring with environmental DNA for marine fish species: “Fish survey project using environmental DNA”
Authors: Suzuki‐Ohno Yukari , Tanabe Akifumi S. , Kasai Akihide , Masuda Reiji , Seino Satoquo , Dazai Akihiro , Suzuki Shota , Abe Takuzo , Kondoh Michio 
Published: April 2023

AbstractEnvironmental DNA is a powerful tool for community science‐based biodiversity surveys. However, the effectiveness of environmental DNA for environmental education and the time and physical costs perceived by volunteers for collecting environmental DNA remain unclear. Here, we evaluated a community science program for monitoring marine fish biodiversity using environmental DNA metabarcoding. This program aimed to investigate marine fish biodiversity in coastal areas along the Japanese archipelago. The participants were allowed to decide on the date and site to collect environmental DNA. They received a paper manual, a data sheet, and a sampling kit via a parcel delivery service. Before collecting environmental DNA, they watched a video manual for collecting environmental DNA and attended a webinar about the process and precautions for collecting environmental DNA provided by the scientists. At the sampling sites, they obtained environmental DNA samples from seawater themselves and sent the samples to the scientists via a refrigerated parcel delivery service. After collecting environmental DNA, they received fish name data from their samples and attended a webinar about survey results provided by the scientists. A cumulative total of 168 volunteers (84 pairs) participated in the program and detected 572 taxonomic groups of fish environmental DNA in the summer of 2020 and 2021. According to a questionnaire survey, more than 75% of the respondents answered that the project improved their understanding of biodiversity, marine environments, and environmental DNA. Approximately 95% of the respondents thought that environmental DNA collection work was meaningful to them. Some respondents commented on the difficulty of interpreting their fish name data, or the time and effort of selecting a sampling site. Therefore, improving the methods to communicate more information about fish name data and select sampling sites will further develop community science monitoring using environmental DNA.