bluuebunny/crossref_metadata_2025_split huggingface dataset:
Intraspecific variation in realized dispersal probability and host quality shape nectar microbiomes
Authors: Francis Jacob S. , Mueller Tobias G. , Vannette Rachel L. 
Published: August 2023

Summary

Epiphytic microbes frequently affect plant phenotype and fitness, but their effects depend on microbe abundance and community composition. Filtering by plant traits and deterministic dispersal‐mediated processes can affect microbiome assembly, yet their relative contribution to predictable variation in microbiome is poorly understood.

We compared the effects of host‐plant filtering and dispersal on nectar microbiome presence, abundance, and composition. We inoculated representative bacteria and yeast into 30 plants across four phenotypically distinct cultivars of Epilobium canum. We compared the growth of inoculated communities to openly visited flowers from a subset of the same plants.

There was clear evidence of host selection when we inoculated flowers with synthetic communities. However, plants with the highest microbial densities when inoculated did not have the highest microbial densities when openly visited. Instead, plants predictably varied in the presence of bacteria, which was correlated with pollen receipt and floral traits, suggesting a role for deterministic dispersal.

These findings suggest that host filtering could drive plant microbiome assembly in tissues where species pools are large and dispersal is high. However, deterministic differences in microbial dispersal to hosts may be equally or more important when microbes rely on an animal vector, dispersal is low, or arrival order is important.
A point mutation in the rice alpha‐tubulin gene OsTUBA3 causes grain notching
Authors: Xu Chenshan , Chen Bingtang , Huang Shanjin , Deng Zhuyun , Wang Tai 
Published: August 2023

Summary

Grain notching is a common deformation that decreases rice (Oryza sativa) quality; however, the underlying molecular basis causing grain notching remains unclear.

We report mechanisms underlying grain notching in Small and notched grain (Sng) mutants, which contained an arginine to histidine substitution at amino acid position 422 (R422H) of the α‐tubulin protein OsTUBA3. The R422H mutation decreased cell length and increased cell width/height of glumes and caryopses, but led to elongated caryopses compressed within shortened glumes, thus giving rise to notched and small grains. Glume and caryopsis cells had different dimensional orientations relative to the directions of organ elongation. Thus, the abnormal cell expansion induced in glumes and caryopses by the R422H mutation had different effects on elongation of these organs.

The R422H mutation in OsTUBA3 compromised β‐tubulin binding and led to formation of defective heterodimers. This in turn affected tubulin incorporation and microtubule (MT) nucleation and regrowth, consequently leading to MT instability and reducing the transverse orientation. The defective MT dynamics affected cell expansion and shape, causing different alterations in glume and caryopsis dimensions and resulting in grain notching.

These data indicate that Arg422 in OsTUBA3 is crucial for MT dynamics and that substitution with His causes grain notching, reducing grain quality and yield. These findings offer valuable insights into the molecular regulation underlying grain development in rice.
Communication strategies and effectiveness of early childhood obesity‐related prevention programs for linguistically diverse communities: A rapid review
Authors: Ahern Sinead , Marshall Sarah , Wallbank Geraldine , Jawad Danielle , Taki Sarah , Baur Louise A. , Wen Li Ming 
Published: August 2023

SummaryChildren from culturally and linguistically diverse backgrounds experience higher rates of obesity and have poorer outcomes in obesity prevention studies. Interventions tailored to specific cultural groups may be limited within linguistically diverse, multicultural communities, and thus, alternative approaches to childhood obesity prevention in these communities are needed. This study aims to describe communication strategies used in interventions targeting prevention of obesity/obesity‐related behaviors, among children 0–5 years, from linguistically diverse communities, and assess their effectiveness. A rapid review was conducted by systematically searching Medline, Embase, and CINAHL. The inclusion criteria are as follows: Studies reported an intervention tailored to linguistically diverse communities targeting at least one obesity‐related behavior among children 0–5 years. The exclusion criteria are as follows: Interventions used simple language translations, targeted one language group, or treated obesity. A total of 4677 articles were identified with 14 studies meeting inclusion criteria. Key communication strategies included materials in multiple languages, English text written at a set readability level, and multimodal delivery. Six studies reported effectiveness data, of which five had effective primary or secondary outcomes. This is the first rapid review to identify communication strategies used in childhood obesity prevention interventions for linguistically diverse communities, highlighting a need for future research to incorporate and evaluate the communication strategies identified.
Derrida and the exemplarity of literature
Authors: Toft Kristian Olesen 
Published: August 2023

AbstractJacques Derrida's scattered remarks on the ambiguous role examples play in the passage between the universal and the singular revolve around an often‐neglected point: any attempt to theorise exemplarity will itself be subject to the law it seeks to account for. This oversight limits scholarship on the subject, but may be amended by returning to the loci classici on exemplarity in Derrida's Glas, La vérité en peinture, Passions, and elsewhere. Moreover, in three texts published in the 1980s: La loi du genre, Préjugés and Psyché, Derrida emphasises how literature is particularly given to ‘remark’ its own status as literature and as such provides a privileged example of the problem of exemplarity. Another example in which exemplarity is in general singularly implicated is deconstruction itself.
Checkpoint kinase 1 inhibitor + low‐dose hydroxyurea efficiently kills BRAF inhibitor‐ and immune checkpoint inhibitor‐resistant melanomas
Authors: Zeng Zhen , Ngo Hung Long , Proctor Martina , Rizos Helen , Dolcetti Riccardo , Cruz Jazmina Gonzalez , Wells James W. , Gabrielli Brian 
Published: August 2023

AbstractTreatment of melanomas with targeted and immunotherapies has proven effective, but resistance to both treatments is a common outcome leaving a high proportion of patients without effective alternative treatment options. Replication stress is a common feature of melanomas, and this is effectively targeted using a combination of checkpoint kinase 1 (CHK1) inhibitor and low‐dose hydroxyurea (LDHU). This combination also promotes inflammatory and anti‐tumour immune responses in vivo. Melanoma cell lines resistant to BRAF inhibitor (BRAFi) or immune checkpoint inhibitors (ICI) retain their sensitivity to CHK1i + LDHU, with sensitivity similar to that of parental tumours. In vivo, BRAFi‐resistant and BRAFi‐sensitive parental tumours produce an identical immune response with treatment.
Effect of cornstalk biochar on phytoremediation of Pb‐contaminated soil by females and males ofPopulus deltoides(Salicaceae)
Authors: Su Yan , Peng Shuming , Xu Gang , Gao Qiao , Chen Juan , Lu Xuyang , Duan Baoli 
Published: August 2023

AbstractSoil pollution with lead (Pb) has become a serious global concern, adversely affecting the forest ecosystem. This study was conducted to investigate the effects of corn straw on the remediation efficiency of Pb‐contaminated soil usingPopulus deltoides. Female and maleP. deltoidescuttings were subjected to soil spiked with 900 mg kg−1Pb and amended with 5% (v/v) corn straw biochar for 90 days. Under Pb stress, the addition of biochar significantly increased the total biomass accumulation by 29% in females and 26% in males. However, without the addition of biochar, the biomass accumulation was significantly reduced by 11% in females and 3% in males under Pb stress. Females showed a higher uptake and accumulation of Pb in roots and leaves, while males accumulated more Pb in roots and stems and exhibited an increased anti‐oxidative capacity. Biochar addition alleviated Pb toxicity in both male and femaleP. deltoidesby immobilizing Pb ion in the soil, reducing Pb uptake and translocation, promoting nutrient uptake, and improving the diversity and stability of the soil bacteria community. Under Pb stress, the relative abundances of metal‐resistance bacteria significantly increased, such as the abundance of Bacteroidetes in females and the abundances of Actinobacteria, Firmicutes, and Planctomycetes in males. In brief, the males under biochar addition exhibited promising potential as candidates for phytoremediation of Pb‐contaminated soil. This study provides new insights into mechanisms underlying sexually differential responses to Pb stress in the presence of biochar amendment.
Immune disorders induced by improper use of dietary immunostimulants in aquatic animals: Research progress and prospective solutions by targeting gut microbiota
Authors: Zhang Qingshuang , Yang Hongwei , Teame Tsegay , Ran Chao , Yang Yalin , Yao Yuanyuan , Ding Qianwen , Liu Shubin , Li Shengkang , Zhang Zhen , Zhou Zhigang 
Published: August 2023

AbstractImmunostimulants were widely used in aquafeed due to their multiple important functions, including promoting growth, enhancing non‐specific defence mechanisms and resisting infectious diseases. But the efficiencies of the positive effects of immunostimulants were affected by the duration of the administration and their dose. Short‐term and normal‐dose of immunostimulants promoted the growth and enhance non‐specific immunity of aquatic animals. However, improper use led to several immune disorders, including immune tolerance, immune exhaustion and over‐immunity. The application of two immunostimulant diets in rotation technique and the intermittent use of immune stimulant diets may solve the problems associated with immune disorders, but this application method will be limited by both species and immunostimulants. The occurrence of immune disorders is accompanied by different degrees of gut microbiota dysbiosis, especially significantly up‐regulated the abundance of Proteobacteria. Besides, the gut symbiotic microbiota plays a significant role in host immunity. Consequently, the regulation of gut microbiota may be an effective way to modulate immune disorders caused by improper use of polysaccharide immunostimulants. Regulating the gut microbiota not only solves the negative effects caused by improper use of immunostimulants without restrictions on both species and immunostimulants, but also has the advantage of simplicity and convenience compared to conventional approaches. In summary, this article reviews the possible negative effects caused by improper use of immunostimulants on aquatic animals and compares regular approaches with regulating gut microbiota. And gut microbiota maybe be used as a therapeutic target to improve immune disorders caused by immunostimulants in aquatic animals.
Hypoglycaemia management with a hypercaloric supplementation in neonatal puppies delivered by caesarean section
Authors: Fuchs Kárita da Mata , Pacífico Pereira Keylla Helena Nobre , de Sousa Gabriela Carneiro , Xavier Gleice Mendes , Camargo Gabriel de Azevedo , de Souza Fabiana Ferreira , Lourenço Maria Lucia Gomes 
Published: August 2023

AbstractThere is a high perinatal mortality rate in dogs, estimated at 20%, and one of the leading causes of this rate is hypoglycaemia. Therefore, we aimed to evaluate the efficacy of a hypercaloric supplement containing vitamins and amino acids in newborn puppies presenting hypoglycaemia at birth. Ninety‐nine pups were divided into four groups: normoglycaemic caesarean section (NORMOCS), hypoglycaemic caesarean section supplemented with the hypercaloric (SUPLCS), hypoglycaemic caesarean section supplemented with glucose (GLICCS) and eutocic delivery (EUT). We evaluated the following parameters glycaemia, Apgar score, neurological reflexes and rectal temperature of neonates at the following moments 5 min (M5), 30 min (M30) and 60 min (M60) after birth. Brachycephalic dogs were 73.3% (22/30) of caesarean sections (c‐sections). The puppy's average glycaemia represented about 90% of the maternal glycaemia, while 15.1% (14/99) of the neonates had hypoglycaemia (<90 mg/dL) at M0 and 46.5% (44/99) at M60. Only four neonates had glycaemia below 40 mg/dL at M30 but without showing any clinical signs. The puppy's fasting while waiting for the intraoperative period and the dam's anaesthetic recovery was considered risk factors for hypoglycaemia. There was no difference in mean blood glucose levels or vitality parameters among puppies from the SUPLCS and GLICCS. In conclusion, the hypercaloric supplement can be used as a replacement for glucose in hypoglycaemic puppies and it can also bring nutritional benefits for the puppy. The prepartum glycaemia of the dam is an important parameter to be measured, and the appropriate management of it reduces the chances of the puppies being born with hypoglycaemia.
Dental management of Kartagener syndrome: A case report
Authors: Abed Hassan , Gogandi Huda , Almutawwif Mustafa , Aloufi Abdullah , Tashkandi Mustafa , Alqarni Ali , Aladwani Fahad , Sadek Hisham S. 
Published: August 2023

AbstractBackgroundKartagener syndrome (KS) is recognized as an inherited, autosomal recessive disorder characterized by a combination of chronic sinusitis, bronchiectasis, and situs inversus. It affects one in 12,500–50,000 live births worldwide.AimThis paper aims to discuss the dental management of patients diagnosed with KS.Case ReportA 31‐year‐old male with KS manifests by impaired cilia motility which increases the risk of a frequent lung infection. The dental examination revealed that the patient required comprehensive oral hygiene care which included patient education and nonsurgical periodontal therapy under local anesthesia.ConclusionsDental care providers should ask affected patients with KS about their signs and symptoms of cardiac and pulmonary disease and seek consultation with their attending physician regarding these health concerns before the initiation of general anesthesia and perhaps conscious sedation administration. Patients with KS with emerging cardiac and/or respiratory impairment should be referred promptly for medical assessment.
Income taxation and progressivity: A measure of equitability
Authors: Creedy John , Subramanian S. 
Published: August 2023

AbstractThis paper proposes a measure of ‘equitability of taxation’, in the context of progressivity and the income tax. By postulating specifications of ‘extreme equitability’ and ‘extreme inequitability’ of a tax system, the paper advances a measure of tax equitability as the normalised area distance of a ‘tax concentration curve’ from its most inequitable version. The measure is derived, and a procedure is outlined for the decomposition of differences in the measure across regimes into a ‘distribution effect’ and a ‘tax system’ effect. A criterion is established for asserting ‘unambiguously greater equitability’ in comparisons across regimes, in terms of a dominance relation akin to the Lorenz quasi‐ordering. The measure is illustrated with the help of numerical examples.
Emotional actions: A new approach
Authors: Pineda David 
Published: August 2023

AbstractThe recent philosophical literature on emotional action is divided between Humeans, who think that emotional action, for all its peculiarities, can in fact be explained along Humean lines, that is, with belief–desire pairs; and emotionists, who think that emotional actions can only be explained by appealing to emotions and some of their special features. After reviewing this philosophical discussion, I will argue, first, that none of the philosophical accounts of emotional action analysed, whether Humean or emotionist, is satisfactory enough. Second, I will argue that this philosophical debate has reached a stalemate, which does not allow further progress and which has not been able to provide us with a compelling account of such emotional actions where it is not obvious which is the goal involved. Third, I will argue that in order to overcome this stalemate, we need to approach emotional action in a radically different way. Drawing on ideas from some psychologists, I will suggest that the relevant philosophical issue should be whether emotional action is, or is not, goal‐directed. Finally, I will suggest how emotional actions, particularly the most puzzling ones, can be accounted for according to this new approach.
Safety and efficacy of weight‐based ganciclovir dosing strategies in overweight/obese patients
Authors: Miller William , January Spenser , Klaus Jeff , Neuner Elizabeth , Pande Anupam , Krekel Tamara 
Published: August 2023

AbstractBackgroundThe management of cytomegalovirus (CMV) is particularly challenging as both CMV and its usual first‐line treatment, ganciclovir, are associated with neutropenia. Ganciclovir dosing is weight‐based, most commonly utilizing total body weight (TBW). The subsequent high doses of ganciclovir in overweight/obese patients may increase the risk of toxicity. Utilizing adjusted body weight (AdjBW) dosing may help mitigate this risk. Therefore, the objective of this study was to evaluate the difference in toxicity and efficacy between TBW and AdjBW ganciclovir dosing strategies in overweight/obese patients.MethodsThis retrospective study conducted safety and efficacy analyses of ganciclovir courses (≥72 h) used as CMV treatment. The primary safety outcome was the incidence of neutropenia (absolute neutrophil count <1000 cells/μL), and the primary efficacy outcome was a 2‐log decrease in CMV polymerase chain reaction within 4 weeks following ganciclovir initiation. In both analyses, courses were excluded in which ganciclovir was dosed outside of specified renal dosing parameters for >20% of the course.ResultsAmong the 253 courses in the safety cohort, there was no difference in the incidence of neutropenia (17.4% vs. 13.5%, p = .50) in AdjBW compared to TBW dosing. In the 62 courses evaluating efficacy, there was no statistical difference between AdjBW and TBW dosing (60.0% vs. 45.2%, p = .28). No subgroups were identified in which AdjBW dosing was advantageous.ConclusionUtilization of AdjBW ganciclovir dosing did not result in decreased neutropenia or treatment efficacy as compared to TBW dosing. Further studies with larger patient populations would be beneficial to confirm these findings.
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Systematic fungal biomarker or polymerase chain reaction testing in lung transplant recipients: Retrospective analysis to optimize their use
Authors: Boutin Catherine‐Audrey , Ison Michael G 
Published: August 2023

AbstractBackgroundAmong lung transplant recipients, serial bronchoscopies are performed frequently. Often, serial galactomannan (GM), 1,3‐β‐d‐glucan (BDG), and Pneumocystis jirovecii (PJ) testing is performed with these broncho‐alveolar lavages (BALs) as standard of care with limited data to support their routine use.MethodsAfter Institutional Review Board approval, we retrospectively collected all blood and BAL GM, BDG, and PJ test results from January 2015 to July 20, 2022. Primary data collection from the Northwestern Medicine EDW was supplemented by manual chart review.ResultsDuring the study period, 236 lung transplant recipients were cared for by our center. Of these patients, 217 (91.9%) had 1418 GM tests performed; 61 (4.3%) were positive (index ≥1). Fungal cultures were requested for most BAL‐GM (90.7%). Out of duplicates in same BAL, results discrepancy was minimal (3.4%). 172 (72.9%) had BDG tests were performed; 25.6% were positive. Thirteen patients had multiple BDG during one hospitalization (mean 2.3 tests); none of the negative test repeated became positive. Eleven negative BDG were seen in patients with invasive aspergillosis (IA). Note that, 577 PJ testing were performed (direct fluorescent antibody [n = 494] or polymerase chain reaction [PCR] [n = 80], or both [n = 3]) in 174 different patients. None were positive.ConclusionDespite supplemental GM, BDG, and Pneumocystis jirovecii pneumonia PCR being performed routinely on lung transplant recipients undergoing BAL at our center, the data suggests a more tailored approach may be appropriate. There is no role for routine serial testing with these assays during a single hospitalization. BDG confers no added‐value over GM with cultures for IA diagnosis.
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StMLP1, as a Kunitz trypsin inhibitor, enhances potato resistance and specifically expresses in vascular bundles during Ralstonia solanacearum infection
Authors: Wang Bingsen , Wang Yuqi , He Wenfeng , Huang Mengshu , Yu Liu , Cheng Dong , Du Juan , Song Botao , Chen Huilan 
Published: August 2023

SUMMARYMiraculin‐like proteins (MLPs), members of the Kunitz trypsin inhibitor (KTI) family that are present in various plants, have been discovered to have a role in defending plants against pathogens. In this study, we identified a gene StMLP1 in potato that belongs to the KTI family. We found that the expression of StMLP1 gradually increases during Ralstonia solanacearum (R. solanacearum) infection. We characterized the promoter of StMLP1 as an inducible promoter that can be triggered by R. solanacearum and as a tissue‐specific promoter with specificity for vascular bundle expression. Our findings demonstrate that StMLP1 exhibits trypsin inhibitor activity, and that its signal peptide is essential for proper localization and function. Overexpression of StMLP1 in potato can enhance the resistance to R. solanacearum. Inhibiting the expression of StMLP1 during infection accelerated the infection by R. solanacearum to a certain extent. In addition, the RNA‐seq results of the overexpression‐StMLP1 lines indicated that StMLP1 was involved in potato immunity. All these findings in our study reveal that StMLP1 functions as a positive regulator that is induced and specifically expressed in vascular bundles in response to R. solanacearum infection.
FDI, production networks and firm behaviour: Evidence from the South African automotive industry
Authors: Higuchi Yuki , Barnes Justin , Black Anthony , Otsuka Keijiro 
Published: August 2023

AbstractFirms in developing countries can learn advanced technology and management know‐how through foreign direct investment (FDI). The extent (or the lack) of transmission of technology and knowledge depends on the structure of production networks. In the automotive industry, networks consist of vehicle assemblers and their layers of parts suppliers. We used the South African automotive industry as a case study to examine how the behaviour of assemblers and parts suppliers is linked. Based on statistical analysis using the original firm‐level data, we found that the first‐tier suppliers significantly increased their production with the regional expansion of automotive production. However, such growth linkages were not observed among lower‐tier suppliers. Further, we obtained the suggestive evidence that only multinational (and not local) first‐tier firms improved their production efficiency, indicating the importance of production networks among multinationals in transmitting technology.