bluuebunny/crossref_metadata_2025_split huggingface dataset:
Molecular surveillance reveals a potential hotspot of tick-borne disease in Hulunbuir City, Inner Mongolia
Authors: Tian Junhua , Liu Jing , Zhao Hongqing , Chen Xiaomin , Geng Xueqin , Lu Miao , Li Kun 
Published: September 2023

Abstract
A molecular surveillance of tick-borne diseases was performed in Hulunbuir City, Inner Mongolia. A total of 149 ticks including three species (Ixodes persulcatus, Haemaphysalis concinna, and Dermacentor silvarum) were collected. As many as 11 tick-borne bacterial pathogens were identified in them. Some of them have high positive rates. For example, Candidatus Rickettsia tarasevichiae was detected with a high prevalence of 72.48%, while Candidatus Lariskella sp. was detected in 31.54% of ticks. For both Rickettsia raoultii and Anaplasma phagocytophilum, two distinct genotypes were identified based on their phylogenetic trees based on 16S rRNA, gltA, and groEL sequences. Remarkable genetic diversity was also observed for 16S and flaB genes of Borreliella garinii, an agent of Lyme disease. Rickettsia heilongjiangensis causing Far-Eastern spotted fever (2.68%, 4/149), Ehrlichia muris causing human ehrlichiosis (4.70%, 7/149), Borrelia miyamotoi causing relapsing fever (2.01%, 3/149), and Borreliella afzelii causing Lyme disease (2.01%, 3/149) were also detected. Additionally, a previously uncharacterized Anaplasma species closely related to Anaplasma ovis was identified. Herein we name it “Candidatus Anaplasma mongolica”. Based on these results, we propose that Hulunbuir City, as well as the northeast part of Inner Mongolia, might be a potential hotspot of tick-borne diseases.
Selective medial septum lesions in healthy rats induce longitudinal changes in microstructure of limbic regions, behavioral alterations, and increased susceptibility to status epilepticus
Authors: Luna-Munguia Hiram , Gasca-Martinez Deisy , Garay-Cortes Alejandra , Coutiño Daniela , Regalado Mirelta , Rios Ericka de los , Villaseñor Paulina , Hidalgo-Flores Fernando , Flores-Guapo Karen , Benito Brandon Yair , Concha Luis 
Published: September 2023

Abstract
Septo-hippocampal pathway, crucial for physiological functions and involved in epilepsy. Clinical monitoring during epileptogenesis is complicated. We aim to evaluate tissue changes after lesioning the medial septum (MS) of normal rats and assess how the depletion of specific neuronal populations alters the animals’ behavior and susceptibility to establishing a pilocarpine-induced status epilepticus. Male Sprague-Dawley rats were injected into the MS with vehicle or saporins (to deplete GABAergic or cholinergic neurons; n = 16 per group). Thirty-two animals were used for diffusion tensor imaging (DTI); scanned before surgery and 14 and 49 days post-injection. Fractional anisotropy and apparent diffusion coefficient were evaluated in fimbria, dorsal hippocampus, ventral hippocampus, dorso-medial thalamus and amygdala. Between scans 2 and 3, animals were submitted to diverse behavioral tasks. Stainings were used to analyze tissue alterations. Twenty-four different animals received pilocarpine to evaluate the latency and severity of the status epilepticus two weeks after surgery. Eight animals were only used to evaluate the extent of neuronal damage inflicted on the MS one week after the molecular surgery. Progressive changes in DTI parameters in both white and gray matter structures of the four evaluated groups were observed. Behaviorally, the GAT1-saporin injection impacted spatial memory formation, while 192-IgG-saporin triggered anxiety-like behaviors. Histologically, the GABAergic toxin also induced aberrant mossy fiber sprouting, tissue damage and neuronal death. Regarding the pilocarpine-induced status epilepticus, this agent provoked an increased mortality rate. Selective septo-hippocampal modulation impacts the integrity of limbic regions crucial for certain behavioral skills and could represent a precursor for epilepsy development.
The COVID-19 pandemic effect on psychologists’ professional quality of life and mental health
Authors: Kercher Amy , Rahman Jodie , Pedersen Mangor 
Published: September 2023

Abstract
Background: Psychologists are at known risk of work-related stress, secondary trauma, and burnout. The COVID-19 pandemic increased stress and anxiety for communities worldwide and corresponded with an increased demand for mental health services. Our study investigated the impact of COVID-19 on psychologists’ professional quality of life, psychological symptoms and work-related stress in Aotearoa, New Zealand.
Method: Ninety-nine registered psychologists were recruited via NZ professional psychology organisations, representing 3% of the total workforce. Survey data collected included symptoms of compassion fatigue and satisfaction, psychological symptoms, COVID-19-related stress and resilience, and professional and personal circumstances during the third year of the pandemic, 2022.
Results: Seventy per cent reported that their work stress had increased, and 60% that their caseload intensity had increased during COVID-19. Psychologists reported receiving little to no additional personal or professional support, while 55% reported increased personal responsibilities during the pandemic (for example, closed childcare and schools during lockdowns). High rates of compassion fatigue (burnout and secondary traumatic stress) were reported, and low resilience. We observed that psychological distress was higher than the community averages before the pandemic and comparable with frontline healthcare professionals. Compassion fatigue was associated with COVID-related stress, psychological distress, years in practice and more frequent supervision, but not with working with at-risk clients, levels of personal support or having children at home. Despite these difficulties, high Compassion Satisfaction scores were also reported, with over 90% indicating they had no intention of leaving the profession in the foreseeable future.
Conclusions: Compassion Fatigue rates appear to have worsened during the COVID-19 pandemic, as have psychological distress symptoms among psychologists. Our findings suggest that mental health workforces are not immune to crisis-related changes. We hope that increased awareness and understanding of psychological difficulties experienced during COVID-19 can be used to tackle future crises better, and better support mental health professionals.
Impact of ocean acidification on bioactive compounds production by marine phytoplankton, Off Visakhapatnam, Bay of Bengal
Authors: Rao D. Bhaskara , Kumar BSK , GVM Gupta 
Published: September 2023

Abstract
Shallow coastal regions face heightened vulnerability due to human development, making them susceptible to substantial influxes of human-caused inputs alongside waters with low pH levels. This research delved into a microcosm pH alteration experiment to explore the impact of pH reduction on the generation of bioactive substances by marine phytoplankton in the eutrophic coastal waters of the Bay of Bengal. Initially, the prevalent compounds in the surface seawater were fucoxanthin at 75%, zeaxanthin at 10%, and other bioactive elements like diadinoxanthin, diatoxanthin, and β-carotene collectively contributing to around 15%. Notably, all bioactive compounds and Chl-a concentrations significantly favored the control container (ranging from 35–70%), while the least growth occurred in the more acidified experimental containers (15–40%). In alignment with the above findings, the nutrient uptake rates were comparably diminished in the acidified experimental containers compared to the control group. The ratio between protective bioactive compounds (Diato + Diadino + Zea + β-Car) and synthetic bioactive compounds (Fuco + Chl-a) varied from 0.03 to 0.8, with the control container exhibiting the lowest values, and the more acidified experimental containers displaying the highest values of significance. Similarly, the DT index (diatoxanthin / (diatoxanthin + diadinoxanthin)) ratios followed a parallel pattern, with the control container showing the lowest average ratios and the acidified experimental containers displaying the highest ratios. Furthermore, based on our current study, we postulated that acidified water stimulates the proliferation of carotenoid-based bioactive compounds in marine regions more prominently than their synthetic counterparts. Mainly, the production of bioactive compounds in these experiments could also be influenced by our acidification method.
Comprehensive and Comparative Survey of Microbial Fuel Cell Performance in the Presence of Graphite and Carbon Felt Electrodes Modified with Nano Bio-Nickel Thin Film
Authors: Mahmoodzadeh Fatemeh , Navidjouy Nahid , Alizadeh Saber , Rahimnejad Mostafa 
Published: September 2023

Abstract
Microbial fuel cells (MFCs) have garnered significant interest from researchers as a self-sustaining and environmentally friendly system for the simultaneous treatment of urban and industrial wastewater and production of bioelectricity. The type and material of the electrode are critical factors that can influence the efficiency and energy production of this treatment process, with porosity, surface area, conductivity, and stability being key considerations. In this study, graphite plates and carbon felt were modified through the electrodeposition of nickel followed by the formation of a biofilm, resulting in conductive bio-anode thin film electrodes with enhanced power generation capacity. The structural and morphological properties of the electrode surfaces were characterized using X-ray diffraction (XRD), energy-dispersive X-ray spectroscopy (EDX), elemental mapping, and field-emission scanning electron microscopy (FE-SEM) techniques. The results confirmed that nickel was successfully doped and that sufficient amounts of microorganisms were attached to the nickel-deposited electrodes, leading to improved electron transfer and increased power generation. To validate this claim, various parameters such as maximum voltage, current density, and power generation were investigated using a dual-chamber MFC equipped with a Nafion 117 membrane and bio-nickel-doped carbon felt (bio-Ni@CF) and bio-nickel-doped graphite plate (bio-Ni@GP) electrodes under constant temperature conditions. The polarization curve obtained during four loading stages using different anode electrodes indicated that the maximum voltage achieved was 468.0 mV using the bio-Ni@CF electrode, representing an increase of 35.0%, 18.37%, and 9.82% compared to the bare GP, bare CF, and bio-Ni@GP electrodes, respectively. The highest power density and current density achieved using the bio-Ni@CF electrode were 130.72 mW/m2 and 760.0 mA/m2, respectively. Furthermore, the modified electrodes demonstrated appropriate stability and resistance during successful runs. These results suggest that nickel-doped carbon-based electrodes can serve as suitable and stable supported catalysts and conductors for improving efficiency and increasing power generation in MFCs.
Desmoplasia in cervical cancer is associated with a more aggressive tumor phenotype
Authors: Wolf Benjamin , Weydandt Laura , Dornhöfer Nadja , Hiller Grit Gesine Ruth , Höhn Anne Katrin , Nel Ivonne , Jain Rakesh K. , Horn Lars-Christian , Aktas Bahriye 
Published: September 2023

Abstract
In cancer of the uterine cervix, the role of desmoplasia, i.e., peritumoral stromal remodeling characterized by fibroblast activation and increased extracellular matrix deposition, is not established. We conducted a retrospective cohort study based on data from 438 patients who had undergone surgical treatment for cervical cancer as part of the prospective Leipzig Mesometrial Resection study between 1999 and 2021. Using non-parametric tests, Kaplan-Meier plotting, and Cox regression modeling, we calculated the prognostic impact of desmoplasia and its association with other risk factors. Desmoplasia was present in 80.6% of cases and was associated with a higher frequency of lymphovascular space involvement (76.5 vs. 56.5%, p < 0.001) and venous infiltration (14.4 vs. 2.4%, p < 0.001). Lymph node metastasis (23.0 vs. 11.8%, p < 0.05) and parametrial involvement (47.3 vs. 17.6%, p < 0.0001) were also more common in patients with desmoplasia. The presence of desmoplasia was associated with inferior overall (80.2% vs. 94.5% hazard ratio [HR] 3.8 [95%-CI 1.4–10.4], p = 0.002) and recurrence-free survival (75.3% vs. 87.3%, HR 2.3 [95%-CI 1.2–4.6], p = 0.008). In addition, desmoplasia was associated with significantly less peritumoral inflammation (rho − 0.43, p < 0.0001). In summary, we link desmoplasia to a more aggressive phenotype of cervical cancer, reduced peritumoral inflammation, and inferior survival.
Facilitators and Barriers of Midwife-led Model of care at Public Health Institutions of Dire Dawa city, Eastern Ethiopia, 2021 A qualitative study
Authors: Hailu Mickiale , Mohammed Aminu , Tadesse Daniel , Abdurashid Neil , Abera Legesse , Ali Samrawit , Dejene Yesuneh , Weldeamaniel Tadesse , Girma Meklit , Hailemariyam Tekelebirhan , Melkamu Netsanet , Getnet Tewodros , Manaye Yibekal , Derese Tariku , Yigezu Muluken , Dechasa Natnael , Atle Anteneh 
Published: September 2023

Abstract

Background: The midwife-led model of care is woman-centered and based on the premise that pregnancy and childbirth are normal life events, and the midwife plays a fundamental role in coordinating care for women and linking with other health care professionals as required. Worldwide, this model of care has made a great contribution to the reduction of maternal and child mortality. For example, the global under-5 mortality rate fell from 42 deaths per 1,000 live births in 2015 to 39 in 2018. The neonatal mortality rate fell from 31 deaths per 1,000 live births in 2000 to 18 deaths per 1,000 in 2018. Even if this model of care has a pivotal role in the reduction of maternal and newborn mortality, in recent years it has faced many challenges.
Objective: To explore facilitators and barriers to a midwife-led model of care at a public health institution in Dire Dawa, Eastern Ethiopia, in 2021.
Methodology: A qualitative study with the supporting philosophy of phenomenology was conducted at Dire Dawa public health institution from March 1–April 30, 2022. Data was collected using a semi-structured, in-depth interview tool guide, focused group discussions, and key informant interviews. A convenience sampling method was implemented to select study participants, and the data were analyzed thematically using computer-assisted qualitative data analysis software Atlas.ti7. The thematic analysis with an inductive approach goes through six steps: familiarization, coding, generating themes, reviewing themes, defining and naming themes, and writing up.
Result: Two major themes were driven from facilitators of the midwife-led model of care (professional pride and good team spirit), and seven major themes were driven from barriers to the midwife-led model of care (lack of professional development, shortage of resources, unfair risk or hazard payment, limited organizational power of midwives, feeling of demoralization absence of recognition from superiors, lack of work-related security).
Conclusion and recommendation: The midwifery-led model of care is facing considerable challenges, both pertaining to the management of the healthcare service locally and nationally. A multidisciplinary and collaborative effort is needed to solve those challenges.
Exploration of the Shared Gene and Molecular Mechanisms between Breast Cancer and Non-alcoholic Fatty Liver Disease Based on Available Public Transcriptome Sequencing Data
Authors: Chen Buyang , Tian Nan , Qian Ying , Li Jie , Wang Qi , Yu Zhiling , Zhao Hong , Dou Xiaobing 
Published: September 2023

Abstract
Background: Breast cancer (BC) is one of the most common malignant tumors in women; its etiology is unknown. A meta-analysis demonstrates a close association between non-alcoholic fatty liver disease (NAFLD) and BC. However, the mechanism of their association remained unknown. The present study aimed to investigate the associations between BC and NAFLD.
Method: The transcriptome sequence data on BC and NAFLD were downloaded from the The Cancer Genome Atlas Program (TCGA) and Gene Expression Omnibus (GEO) databases, respectively. The co-expression modules related to BC and NAFLD were identified using Weighted Gene Co-Expression Network Analysis (WGCNA). ClueGo software was used for enrichment analysis on BC and NAFLD common genes. Moreover, the common microRNAs (miRNAs) in BC and NAFLD were obtained from the Human microRNA Disease Database (HMDD), and the target genes of these miRNAs were predicted using the miRTarbase. Disease enrichment was performed using lncRNA from the Starbase. We then constructed the common miRNAs–mRNAs network. In addition, we obtained a correlation analysis of common genes and BC unique genes.
Result: Several modules were identified as significant with BC and NAFLD based on WGCNA results. ClueGO enrichment analysis revealed that vasculature development is a feature shared by the pathophysiology of BC and NAFLD. The miRNA enrichment BP and lncRNA disease enrichment analyses revealed a link between BC and NAFLD. Furthermore, we identified three BC features that may be involved in the transition from NAFLD to BC and disease-crucial genes FOXO1 and PKD2.
Conclusion: Our study revealed that vasculature development may be an important point for BC and NAFLD, with parts of BC patients evolving from NAFLD by three group feature genes. Furthermore, these gene modules could be biomarkers or potential diagnostic targets in NAFLD patients.
Thermoplastic compression is a technique of pure titanium microstructure enhancement for biomedical applications
Authors: Bańczerowski Jakub , Pawlikowski Marek , Płociński Tomasz , Zagórski Andrzej , Sawicki Sylwester , Gieleta Roman 
Published: September 2023

Abstract
Pure titanium due to its high corrosion resistance, low stiffness and good mechanical properties is commonly used in medicine for orthopaedic applications. However, its material properties can be further enhanced to better fulfil the role of the biocompatible material. The thermoplastic deformation in heightened temperature is proposed as a method for microstructure improvement. Titanium samples were compressed in different temperatures and strain rates in order to determine the best conditions for grain fragmentation – the main factor responsible for strength and hardness increase. The thermoplastic stress-strain curves were registered. Then microstructure observations and electron backscatter analysis were performed on the chosen samples. Finally, mechanical response of the previously deformed material was obtained in room temperature compression tests. A significant grain fragmentation was recorded for the material deformed in 400 °C, at 0.1/s and 1/s strain rates. Desirable results were also noticed for the deformation performed at 500-600 °C. However, high temperatures (700-800 °C) and strain rates (10/s) resulted in dynamic recrystallization, causing undesirable grain growth. An increase in hardness was observed in all cases, with higher values recorded in lower deformation temperatures. Room temperature compression tests revealed slight increase of ductility.
Comparative analysis of multiple nutritional scoring tools to predict the incidence of adverse events in elderly patients with acute coronary syndrome after PCI
Authors: Zhu Xing-Yu , Yang Dan-Dan , Zhang Kai-Jie , Zhu Hui-Jing , SU Fei-Fei , Tian Jian-Wei 
Published: September 2023

Abstract
To determine the most appropriate nutritional assessment tool for predicting the occurrence of major adverse cardiovascular events (MACE) within 1 year in elderly ACS patients undergoing PCI from four nutritional assessment tools including PNI, GNRI, CONUT, and BMI. Consecutive cases diagnosed with acute coronary syndrome (ACS) and underwent percutaneous coronary intervention (PCI) in the Department of Cardiovascular Medicine of the Air Force Specialty Medical Centre from 1 January 2020 to 1 April 2022 were retrospectively collected. The basic clinical characteristics and relevant test and examination indexes were collected uniformly, and the cases were divided into the MACE group (174 cases) and the non-MACE group (372 cases) according to whether a major adverse cardiovascular event (MACE) had occurred within 1 year. Predictive models were constructed to assess the nutritional status of patients with the Prognostic Nutritional Index (PNI), Geriatric Nutritional Risk Index (GNRI), Controlling nutritional status (CONUT) scores, and Body Mass Index (BMI), respectively, and to analyze their relationship with prognosis. The incremental value of the four nutritional assessment tools in predicting risk was compared using the Integrated Discriminant Improvement (IDI) and the net reclassification improvement (NRI). The predictive effect of each model on the occurrence of major adverse cardiovascular events (MACE) within 1 year in elderly ACS patients undergoing PCI was assessed using area under the ROC curve (AUC), calibration curves, decision analysis curves, and clinical impact curves; comparative analyses were performed. Among the four nutritional assessment tools, the area under the curve (AUC) was significantly higher for the PNI (AUC: 0.798, 95%CI: 0.755-0.840 P < 0.001) and GNRI (AUC: 0.760, 95%CI .0.715-0.804 P < 0.001) than for the CONUT (AUC: 0.719,95%CI. 0.673-0.765 P < 0.001) and BMI (AUC:0.576, 95%CI:0.522-0.630 P < 0.001). The positive predictive value (PPV) of PNI: 67.67% was better than GNRI, CONUT, and BMI, and the negative predictive value (NPV): of 83.90% was better than CONUT and BMI and similar to the NPV of GNRI. The PNI, GNRI, and CONUT were compared with BMI, respectively. The PNI had the most significant improvement in the Integrated Discriminant Improvement Index (IDI) (IDI: 0.1732, P < 0.001); the PNI also had the most significant improvement in the Net Reclassification Index (NRI) (NRI: 0.8185, P < 0.001). In addition, of the four nutritional assessment tools used in this study, the PNI was more appropriate for predicting the occurrence of major adverse cardiovascular events (MACE) within 1 year in elderly ACS patients undergoing PCI.
A highly selective and sensitive flavonoid fluorescent probe for the detection of Al3+ in aqueous environments and its application in cellular imaging
Authors: An Chaona , Li Hengyi , liu Cunfang , Liu Di , Wang Wenlong , Zhang Chenyang , Zhao Bailin , Liu Bo , Tian Guanghui 
Published: September 2023

Abstract
Aluminum (Al), which is not an essential element in maintaining the living system could cause chronic toxicity. The specific and sensitive detection of Al3+ is a prerequisite to evaluating its risk to life. We rationally designed and synthesized a novel bromoflavonol-based fluorescent probe IV, which shows a blue shift of the emission and fluorescence enhancement after binding to Al3+, and thus could be used for Al3+ detection. UV-Vis absorption and fluorescence emission spectra show that Ⅳ has high selectivity and sensitivity to Al3+ and exhibits strong immunity to other metal ions. The detection limit(LODs) of Ⅳ to Al3+ is 1.8×10− 8 mol/L as deduced from the fluorescence titration. The binding stoichiometry of Ⅳ to Al3+ is 1:1 as deduced from Job curve and 1H NMR titration. The HOMO and LUMO energy gap difference between Ⅳ and Ⅳ་Al3+ was calculated by DFT (generalized functional theory), and the structure of the complex formed with probe IV and Al3+ is more stable than that of IV. Al3+ was detected in tap water and river water in the middle regions of the Han River with recoveries of more than 96% by using this probe, which can realize the quantitative detection of Al3+ in environmental water samples. In addition, the probe can be successfully used for imaging of Al3+ in the MG63 cells, indicating a potential application of this fluorescence probe in biological imaging.