bluuebunny/crossref_metadata_2025_split huggingface dataset:
A novel electronic patient-reported outcome delivery system to implement health-related quality of life measures in routine clinical care: An analysis of 5 years of experience
Authors: Tymms Kathleen , O'Sullivan Catherine , Smith Tegan , Littlejohn Geoffrey , Freeman Tim , Hoffman David , Segelov Dana , Griffiths Hedley , Ciciriello Sabina , Youssef Peter , Mathers David , Deakin Claire T 
Published: January 2023

ObjectiveTo develop a simple and secure technological solution to incorporate electronic patient-reported outcomes (ePROs) into routine clinical care.MethodsA novel ePRO questionnaire delivery system was developed by Software for Specialists (S4S) in partnership with OPAL Rheumatology Australia. Validated questionnaires were sent from the electronic medical record (EMR) (Audit4) of patients with rheumatoid arthritis (RA), psoriatic arthritis (PsA), ankylosing spondylitis (AS), lupus or giant cell arteritis (GCA) and delivered to the patient's email address or completed in the clinic waiting room using a smart device (in-practice). Completed questionnaires were encrypted and returned to the patient's Audit4. Deidentified clinical data was extracted and aggregated across all sites. Data collected between April 2016-Dec 2020 were analysed descriptively.ResultsBetween April 2016 to Dec 2020, 221,352 Functional Assessment of Chronic Illness Therapy Fatigue (FACIT-F), Patient Health Questionnaire-2 (PHQ-2) and/or HealthCare Resource Utilization (HCRU) questionnaires were sent from 39 of 42 contributing clinics (93%). 85% of questionnaires were delivered via email and 15% in-practice. Overall, 85% of patients completed at least one questionnaire, and of all questionnaires sent, 73% were completed. Females were more likely to engage with the questionnaires than males (87% vs. 81%), and older patients were slightly more likely to complete all questionnaires delivered.ConclusionsThe novel Audit4 ePRO delivery system is an effective tool for incorporating PROs into routine clinical care. The data generated provides a unique opportunity to understand the full burden of disease for patients in the real-world setting and the impact of interventions.
Petrology of UHP eclogite-facies felsic schist in the Western Tianshan subduction zone, China
Authors: Zhang Lijuan , Zhang Lifei , Qi Ning , Bader Thomas 
Published: January 2023

Although quartzo-feldspathic metasedimentary rocks are widespread in high pressure-ultrahigh pressure (HP-UHP) metamorphic belts worldwide, their petrogenesis and metamorphic evolution is poorly understood. We discovered an UHP eclogite-facies felsic schist in the Western Tianshan metamorphic belt, China. Petrological observations and phase equilibria modeling both indicate the felsic schist experienced UHP metamorphism in the coesite stability field. In particular, it experienced prograde metamorphism at 21–24 kbar, 445°C–470°C, a pressure peak at 25–28 kbar and 490°C–525°C, and eventually heating with decompression to 20 kbar and 560°C. The obtained clockwise P-T path was consistent with those of other lithologies (eclogite and pelitic schist) from the same belt, which provides new evidence for the coherent exhumation of the UHP unit of the Western Tianshan metamorphic belt. The final uplift of the Western Tianshan oceanic crust to the surface is attributed to fluid activity and late tectonic deformation.
Differential impact of COVID-19 school closures on immigrant students: A transnational comparison
Authors: Finch Holmes , Hernández Finch Maria E. , Avery Brooke 
Published: January 2023

IntroductionThe COVID-19 pandemic had a major impact on many aspects of life, perhaps most notably education. Efforts to mitigate the negative effects of the pandemic, particularly lockdowns, led to major disruptions in schools and resulted in both learning loss and increased mental health challenges for students. These deleterious impacts appear to have been felt most keenly by students from marginalized communities, including first and second generation students.Materials and methodsThis study sought to investigate the mechanisms underlying these negative effects of pandemic mitigation efforts, particularly in terms of school efforts to support teachers and parents of students not speaking the language of instruction for three nations (Denmark, Russia, and Slovenia) included in the Responses to Educational Disruption Survey (REDS) survey.ResultsResults of the study revelated that in Denmark greater school-level support to teachers of non-native language students moderated the relationship between home language status and student perceptions of their relative academic performance before and during the pandemic, but that such was not the case in Russia or Slovenia. Likewise, school-level support to teachers moderated the relationship between home language status and perceived teacher support in Denmark but not in Russia or Slovenia. Implications of these results are discussed.
Systematic review of transcriptome and microRNAome associations with gestational diabetes mellitus
Authors: Lewis Kimberly A. , Chang Lisa , Cheung Julinna , Aouizerat Bradley E. , Jelliffe-Pawlowski Laura L. , McLemore Monica R. , Piening Brian , Rand Larry , Ryckman Kelli K. , Flowers Elena 
Published: January 2023

PurposeGestational diabetes (GDM) is associated with increased risk for preterm birth and related complications for both the pregnant person and newborn. Changes in gene expression have the potential to characterize complex interactions between genetic and behavioral/environmental risk factors for GDM. Our goal was to summarize the state of the science about changes in gene expression and GDM.DesignThe systematic review was conducted using the Preferred Reporting Items for Systematic Reviews and Meta-Analyses guidelines.MethodsPubMed articles about humans, in English, from any date were included if they described mRNA transcriptome or microRNA findings from blood samples in adults with GDM compared with adults without GDM.ResultsSixteen articles were found representing 1355 adults (n=674 with GDM, n=681 controls) from 12 countries. Three studies reported transcriptome results and thirteen reported microRNA findings. Identified pathways described various aspects of diabetes pathogenesis, including glucose and insulin signaling, regulation, and transport; natural killer cell mediated cytotoxicity; and fatty acid biosynthesis and metabolism. Studies described 135 unique miRNAs that were associated with GDM, of which eight (miR-16-5p, miR-17-5p, miR-20a-5p, miR-29a-3p, miR-195-5p, miR-222-3p, miR-210-3p, and miR-342-3p) were described in 2 or more studies. Findings suggest that miRNA levels vary based on the time in pregnancy when GDM develops, the time point at which they were measured, sex assigned at birth of the offspring, and both the pre-pregnancy and gestational body mass index of the pregnant person.ConclusionsThe mRNA, miRNA, gene targets, and pathways identified in this review contribute to our understanding of GDM pathogenesis; however, further research is warranted to validate previous findings. In particular, longitudinal repeated-measures designs are needed that control for participant characteristics (e.g., weight), use standardized data collection methods and analysis tools, and are sufficiently powered to detect differences between subgroups. Findings may be used to improve early diagnosis, prevention, medication choice and/or clinical treatment of patients with GDM.
Spatiotemporal evolution of investment-carbon emission coupling coordination in China’s electricity market
Authors: Zhang Yuan , Zhang Hongyuan , Hao Feng 
Published: January 2023

Promoting the low-carbon development of the electricity market is the key to controlling CO2 emissions and achieving carbon neutrality in China. It requires the coordinated development between investment and carbon emissions in the electricity industry. Based on the panel data on electricity investment and carbon emissions from 2000 to 2019, this study systematically explains the coupling coordination mechanism between electricity investment and carbon emissions. We use the coupling coordination model to calculate the coupling coordination degree of each province. Then, the research uses the GM (1, 1) model to predict the coupling coordination development from 2020 to 2030. The study finds that the development of China’s electricity industry is in good shape. Although the coupling coordination degree has entered barely or primary coordination in most provinces, there are certain fluctuations in recent years; there are spatial differences in coupling and coordinated development among regions: the central region has a high coupling coordination degree, while the eastern and northeastern regions are relatively lagging behind. In the next 10 years, the coupling coordination degree will continue to grow, and all regions will reach the primary coordination. Among them, the central region will reach the intermediate coordination.
Spatial heterogeneity and influence mechanisms on soil respiration in an old-growth tropical montane rainforest with complex terrain
Authors: Yang Huai , Huang Ting , Li Yide , Liu Wenjie , Fu Jialin , Huang Biao , Yang Qiu 
Published: January 2023

IntroductionAlthough numerous studies have investigated ecosystem-scale soil respiration (SR) at different ecosystem, our understanding of spatial heterogeneity of SR at plot scale is still incomplete, especially in tropical rainforests with complex topography. Further, the ecological factors that drive the variability of SR in tropical rainforests is also poorly understood.MethodsHere, we investigated the spatial variations and control mechanisms of SR in a 60-ha plot of old-growth tropical rainforest with complex topography. Specifically, we sampled a 60-ha plot in intervals of 20 m to measure SR with LI-8100, used semi-variogram of geostatistical tools to examine spatial heterogeneity of SR.ResultsThe mean SR rate in this plot was 4.312 ± 0.0410 (SE) μmol m−2 s−1. Geostatistical analysis indicated that the SR rate at this plot had a moderate spatial dependence, with a nugget-to-sill ratio of 68.1%. The coefficients variance of SR was 36.2% and the patch size was approximately 112 m. Stepwise linear regression analysis (involving a multiple regression tree) revealed that the independent factors regulated different types of SR’s. Liner mix-effect models showed that SR was significantly positively related to soil phosphorus and negatively to the slope in the 60-ha plot. Spatial disturbance of SR along multidimensional habitats that an increase in elevation of the multidimensional habitat, which was accompanied by enhanced SOC and soil phosphorous, also increased its SR in the 60-ha plot.DiscussionThis study would be helpful in designing future field experiments for a better understanding of SR at plot scale.
No difference in anti-spike antibody and surrogate viral neutralization following SARS-CoV-2 booster vaccination in persons with HIV compared to controls (CO-HIV Study)
Authors: Kling Kendall D. , Janulis Patrick , Demonbreun Alexis R. , Sancilio Amelia , Berzins Baiba , Krueger Karen , Achenbach Chad , Price Rachelle , Sullivan Margaret , Caputo Matthew , Hockney Sara , Zembower Teresa , McDade Thomas W. , Taiwo Babafemi 
Published: January 2023

BackgroundUnderstanding the immune response to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) vaccination will enable accurate counseling and inform evolving vaccination strategies. Little is known about antibody response following booster vaccination in people living with HIV (PLWH).MethodsWe enrolled SARS-CoV-2 vaccinated PLWH and controls without HIV in similar proportions based on age and comorbidities. Participants completed surveys on prior SARS-CoV-2 infection, vaccination, and comorbidities, and provided self-collected dried blood spots (DBS). Quantitative anti-spike IgG and surrogate viral neutralization assays targeted wild-type (WT), Delta, and Omicron variants. We also measured quantitative anti-nucleocapsid IgG. The analysis population had received full SARS-CoV-2 vaccination plus one booster dose. Bivariate analyses for continuous outcomes utilized Wilcoxon tests and multivariate analysis used linear models.ResultsThe analysis population comprised 140 PLWH and 75 controls with median age 58 and 55 years, males 95% and 43%, and DBS collection on 112 and 109 days after the last booster dose, respectively. Median CD4 count among PLWH was 760 cells/mm3 and 91% had an undetectable HIV-1 viral load. Considering WT, Delta, and Omicron variants, there was no significant difference in mean quantitative anti-spike IgG between PLWH (3.3, 2.9, 1.8) and controls (3.3, 2.9, 1.8), respectively (p-values=0. 771, 0.920, 0.708). Surrogate viral neutralization responses were similar in PLWH (1.0, 0.9, and 0.4) and controls (1.0, 0.9, 0.5), respectively (p-values=0.594, 0.436, 0.706).ConclusionsPLWH whose CD4 counts are well preserved and persons without HIV have similar anti-spike IgG antibody levels and viral neutralization responses after a single SARS-CoV-2 booster vaccination.
Construction of ceRNA and m6A-related lncRNA networks associated with anti-inflammation of AdipoAI
Authors: Yu Hongwen , Wu Hongle , Xie Qiuyan , Liu Zining , Chen Zehao , Tu Qisheng , Chen Jake , Fang Fuchun , Qiu Wei 
Published: January 2023

BackgroundAdiponectin (APN) is an endogenous adipokine secreted from adipocytes that exerts anti-inflammatory properties. AdipoAI is an orally active adiponectin receptor agonist identified by our group that can emulate APN's anti-inflammatory properties through mechanisms that are not fully understood. LncRNAs, a type of noncoding RNA more than 200 bp in length, have been demonstrated to have abundant biological functions, including in anti-inflammatory responses.Materials and ResultIn the current study, we performed a lncRNA microarray in LPS-induced Raw264.7 cells that were prestimulated with AdipoAI and screened 110 DElncRNAs and 190 DEmRNAs. Enrichment analyses were conducted on total mRNAs and DEmRNAs, including GSVA, ssGSEA, GO/KEGG, GSEA, and PPI analysis. Among all these processes, endocytosis was significantly enriched. A coexpression analysis was built based on DElncRNAs and DEmRNAs. Then, using TargetScan and miRwalk to predict related microRNAs of DElncRNAs and DEmRNAs, respectively, we established competing endogenous RNA (ceRNA) networks including 54 mRNAs from 8 GO items. Furthermore, 33 m6A methylation-related marker genes were obtained from a previous study and used for the construction of an m6A-related lncRNA network by coexpression analysis. We identified FTO as the hub gene of the network and 14 lncRNAs that interacted with it. The expression levels of 10 lncRNAs selected from ceRNA and FTO-related lncRNA networks were validated with qRT‒PCR. Finally, macrophage phenotype scores showed that AdipoAI could attenuate the M2b and M2c polarization of macrophages and correlate with the above lncRNAs.ConclusionOur work reveals that lncRNAs might be involved in the anti-inflammation process of AdipoAI in LPS-induced macrophages through the ceRNA network and the epigenetic regulation of m6A. Mechanistically, these lncRNAs associated with AdipoAI might be related to endocytosis and polarization in macrophages and provide new candidates for the anti-inflammatory application of APN and its receptor agonist.
Melanocortin receptor agonist NDP-α-MSH improves cognitive deficits and microgliosis but not amyloidosis in advanced stages of AD progression in 5XFAD and 3xTg mice
Authors: Daini Eleonora , Vandini Eleonora , Bodria Martina , Liao Wenjie , Baraldi Carlo , Secco Valentina , Ottani Alessandra , Zoli Michele , Giuliani Daniela , Vilella Antonietta 
Published: January 2023

IntroductionAlzheimer’s disease (AD) is the most frequent cause of dementia and still lacks effective therapy. Clinical signs of AD include low levels of endogenous melanocortins (MCs) and previous studies have shown that treatment with MC analogs induces neuroprotection in the early stages of AD. MethodsWe investigated the neuroprotective role of MCs in two transgenic mouse models of severe AD using 5 and 7 month-old (mo) 5XFAD mice and 9 and 12 mo 3xTg mice. These mice were subjected to a chronic stimulation of MC receptors (MCRs) with MC analogue Nle4-D-Phe7-α-melanocyte stimulating hormone (NDP-α-MSH, 340 μg/kg, i.p.). Mouse behavior and ex-vivo histological and biochemical analyses were performed after 50 days of treatment.ResultsOur analysis demonstrated an improvement in cognitive abilities of AD mice at late stage of AD progression. We also showed that these protective effects are associated with decreased levels of hyperphosphorylated Tau but not with Aβ burden, that was unaffected in the hippocampus and in the cortex of AD mice. In addition, an age-dependent NDP effect on glial reactivity was observed only in 3xTg mice whereas a global downregulation of p38 mitogen-activated protein kinase was selectively observed in 7 mo 5XFAD and 14 mo 3xTg mice.ConclusionOur results suggest that MCR stimulation by NDP-α-MSH could represent a promising therapeutic strategy in managing cognitive decline also at late stage of AD, whereas the effects on neuroinflammation may be restricted to specific stages of AD progression.
DNA methylation change of HIPK3 in Chinese rheumatoid arthritis and its effect on inflammation
Authors: Jiang Ping , Wei Kai , Xu Lingxia , Chang Cen , Zhang Runrun , Zhao Jianan , Jin Yehua , Xu Linshuai , Shi Yiming , Qian Yi , Sun Songtao , Guo Shicheng , Wang Rongsheng , Qin Yingying , He Dongyi 
Published: January 2023

IntroductionHomeodomain-interacting protein kinase 3 (HIPK3) plays an important role in cell proliferation, apoptosis, and inflammation. Over-expression of HIPK3 in immune cells in rheumatoid arthritis (RA) has been reported. In this study, we investigated blood methylation levels and clinical characteristics of RA in a Chinese population.MethodsA total of 235 patients with RA, 30 with osteoarthritis (OA), and 30 matched healthy controls were recruited. The methylation status of seven CpGs in the differentially methylated region of HIPK3 (cg05501357) was measured using targeted methylation-sequencing technology. The association between methylation haplotypes and the overall methylation status of HIPK3 with clinical characteristics was assessed using generalized linear regression.ResultsAll seven CpGs showed hypomethylation status in RA blood compared with OA and normal individuals (overall p= 1.143×10-8 and FDR= 2.799×10-7), which is consistent with the previously reported high expression of HIPK3 in RA immune cells. Among all seven CpGs, 33286785 showed the highest predictive power with an area under the curve (AUC) of 0.829; we received a higher AUC=0.864 when we combined HIPK3 with anti-citrullinated protein antibodies (ACPA -) and rheumatoid factor (RF +) in the prediction model, indicating that when a patient’s ACPA is negative, HIPK3 can assist RF as a new clinical index for the diagnosis of RA. We also found that HIPK3 methylation levels were negatively correlated with C-reactive protein (CRP; r= -0.16, p= 0.01). Methylation haplotypes were analyzed, and the full methylation haplotype (FMH; r= 0.16, p= 0.01) and full non-methylation haplotype (FNH; r= 0.18, p= 0.0061) were negatively correlated with CRP.ConclusionCirculating blood methylation levels in the protein region of HIPK3 can be utilized as a supportive diagnostic biomarker and CRP level indicator for RA.
Microwave ablation and synchronous transarterial chemoembolization combined with PD-1 inhibitor in patients with hepatocellular carcinoma following tyrosine kinase inhibitor intolerance
Authors: Shi Qin , Zhou Xin , Zhang Zihan , Zhang Wen , Ma Jingqin , Yang Minjie , Yu Jiaze , Luo Jianjun , Liu Lingxiao , Yan Zhiping 
Published: January 2023

PurposeTo determine the safety and efficacy of microwave ablation (MWA) and synchronous transarterial chemoembolization (TACE) combined with or without PD-1 inhibitor in patients with hepatocellular carcinoma (HCC) following tyrosine kinase inhibitor (TKI) intolerance.Materials and methodsThis study retrospectively enrolled TKI-intolerant HCC patients who underwent MWA-TACE combined with PD-1 inhibitor (MTP) or MWA-TACE (MT) from January 2019 to June 2021. MWA and TACE were performed simultaneously, and PD-1 inhibitor was administered intravenously at a dose of 200 mg once every three weeks after MWA-TACE. Adverse events (AEs) related to treatment were recorded during the follow-up. Progression-free survival (PFS) and overall survival (OS) were compared between the two groups.ResultsA total of 87 patients were included and classified into the MTP group (n =42) and MT group (n=45). Complications related to MWA-TACE in the MTP group were similar to that in the MT group (21.4% vs. 24.4%, P = 0.738). Moreover, 35 (83.3%) patients had eighty-four AEs related to PD-1 inhibitor in the MTP group, and 8 (19.0%) patients developed grade 3. Patients who underwent MWA-TACE combined with PD-1 inhibitor had better PFS (median, 10.0 vs. 4.7 months, P &lt; 0.001) and OS (median, 17.0 vs. 8.5 months, P &lt; 0.001) than those who underwent MWA-TACE alone. Treatment method and Child-Pugh class were independent prognostic factors for survival in the univariate and multivariate analysis.ConclusionMWA and synchronous TACE combined with PD-1 inhibitor might be a favorable treatment option in TKI-intolerant HCC patients.
Identification and validation of a prognostic signature of autophagy, apoptosis and pyroptosis-related genes for head and neck squamous cell carcinoma: to imply therapeutic choices of HPV negative patients
Authors: Nan Zhaodi , Dou Yu , Chen Anwei , Wang Ketao , Sun Jintang , Meng Zhen , Neckenig Markus , Ai Dan , Liu Shaohua , Dong Zuoqing , Ma Chao , Cheng Yufeng , Qu Xun 
Published: January 2023

IntroductionAn effective tool is needed to predict the prognosis of head and neck squamous cell carcinoma (HNSCC). Human papillomavirus (HPV) positive HNSCC patients generally have a favorable survival and a promising responsiveness to radiotherapy, chemoradiotherapy and checkpoint blockades. However, HPV negative patients, the majority of HNSCC patients, have been largely overlooked. Cell death has been involved in the therapeutic resistance of cancers. To this end, we aimed to identify the association of autophagy, apoptosis and pyroptosis-related genes with the prognosis of HNSCC, and construct a prognostic signature to predict the prognosis for HNSCC, especially for HPV negative HNSCC.MethodsAutophagy and apoptosis-related genes were obtained from Gene Set Enrichment Analysis (GSEA) website, and pyroptosis-related genes were obtained from GSEA and Gene Ontology (GO) database. We established the cell death index (CDI) based on RNA sequencing (RNA-seq) data and clinicopathological information from The Cancer Genome Atlas (TCGA) dataset. The prognostic value of CDI was verified by Kaplan-Meier, receiver operating characteristic (ROC) and univariate and multivariate Cox regression analyses in TCGA dataset, and validated with the datasets from Gene Expression Omnibus (GEO) and Qilu Hospital of Shandong University. We further assessed the immune microenvironment of patients with high and low CDI scores. Moreover, the expression of the signature genes in HNSCC cell lines were explored.ResultsWe found that CDI was an independent prognostic indicator for overall survival (hazard ratio 3.80, 95% confidential interval: 2.70-5.40, P &lt; 0.001). Furthermore, HNSCC patients with high CDI scores obtained increased overall survival post radiation indicating benefits from radiotherapy of this subgroup. On the other hand, HPV negative HNSCC patients with low CDI exhibited increased checkpoint gene expressions, an inflamed tumor microenvironment and an enriched immune response-related functions, suggesting the potential benefits from checkpoint immunotherapies of this subgroup. Moreover, we validated the baseline and induced expressions of above 16 genes in two HPV negative HNSCC cell lines, CAL27 and SCC-15.DiscussionWe established a prognostic signature and emphasized its implements in the therapeutic choices of HPV negative HNSCC patients, the majority and the poor outcome population of HNSCC.