bluuebunny/crossref_metadata_2025_split huggingface dataset:
PS-BPB10-5: POSSIBLE INVOLVEMENT OF RENAL TUBULAR NFAT5 IN AGING-ASSOCIATED RENAL DYSFUNCTION AND SALT-SENSITIVE HYPERTENSION
Authors: Maruyama Kosuke , Izumi Yuichiro , Ono Makoto , Kakizoe Yutaka , Morinaga Jun , Kuwabara Takashige , Mukoyama Masashi 
Published: January 2023

Objective:
Japan is currently facing a super-aging society, where an incidence of lifestyle-related diseases has become a greater social burden. The number of patients with chronic kidney disease (CKD) and hypertension is being increased with age; however, it is not clearly defined why and how aging causes renal dysfunction and hypertension. Nuclear factor of activated T-cells 5 (NFAT5) is a transcription factor that is activated upon hypertonic conditions as observed in the renal medulla. We have shown that the renal tubular cell-specific NFAT5 conditional knockout (KO) mice exhibit salt-sensitive hypertension, while the mice exhibit impaired urine concentrating ability and are susceptible to renal fibrosis. These phenotypes resemble aging-associated renal dysfunction, i.e., urine concentrating disorder, salt-sensitive hypertension, and renal fibrosis. We therefore investigated the possible involvement of NFAT5 in aging-related changes of the kidney.

Methods:
Male wild-type (WT) and KO mice (12 weeks old) were subjected to unilateral ureteral obstruction (UUO) or sham operation. After 7 days, mice were sacrificed and kidneys were removed for analysis. Renal fibrosis was evaluated by AZAN staining. Senescence-associated beta-galactosidase (SA-β;-Gal) activity was examined by fluorescence study in the whole kidney slices. mRNA expression for senescence-associated secretory phenotype (SASP)-related factors (TGF-β;1, COL1A1, ICAM1, PAI-1, p21, p16, and p53) in the whole kidney tissue was examined by real-time PCR.

Results:
AZAN staining showed that UUO induced renal fibrosis both in WT and KO mice. The UUO-induced fibrotic area was significantly larger in KO mice than in WT mice in the medulla. In sham-operated mice, overall SA-β;-Gal activity was larger in KO mice, in which the activity was dominant in the corticomedullary region. In UUO groups, overall SA-β;-Gal activity was low compared to sham-operated groups. The mRNA expression of SASP-related factors was not different between WT and KO mice in sham-operated groups. UUO upregulated the mRNA expression of all SASP-related factors both in WT and KO mice. In UUO groups, the expression of TGF-β;1, COL1A1, ICAM1, and PAI-1 was significantly larger in KO mice than in WT mice. The expression of p21, p16, and p53 tended to be larger in KO mice than in WT mice.

Conclusions:
Renal tubular NFAT5 deficiency showed senescence-related phenotypes at baseline. Deficit of renal tubular NFAT5 may further induce cell senescence upon insult, which might accelerate aging-associated renal inflammation and dysfunction in CKD.
PS-BPC02-4: RELATIONSHIP BETWEEN EXERCISE CAPACITY AND CHANGES OF PULSE WAVE VELOCITY AFTER CARDIAC REHABILITATION IN PATIENTS WITH ACUTE MYOCARDIAL INFARCTION
Authors: Yoon Homin , An Soyeon , Kang Bomin , Beom Jongwook , Boo Kiyoung , Lee Jaegeun , Choi Joonhyouk , Kim Songyi , Joo Seungjae 
Published: January 2023

Objective:
Guidelines strongly recommend exercise-based cardiac rehabilitation (CR) to reduce cardiovascular mortality in patients with acute myocardial infarction (AMI). Reduction of central aortic systolic pressure, augmentation index, and pulse wave velocity (PWV) after aerobic exercise may be associated with better clinical outcomes after CR. However, the change of pulse wave velocity after CR in patients with AMI has been rarely investigated.

Design and Method:
Sixty-five patients (age 55.3 ± 10.1 years, 63 men) with AMI who completed the 6-month CR and evaluation program were included in this study. Maximal oxygen uptake (VO2max) during cardiopulmonary exercise (CPX) testing and brachial-ankle PWV (baPWV) were measured before exercise program and after 6 months. Based on the results of baseline CPX testing, each exercise program which included target metabolic equivalent, target heart rate, and exercise duration, was prescribed. Patients were recommended to attend the outpatient CR program which composed of 8 to 12 visits during 4 to 6 weeks. Self-exercise program was educated for each patient who finished or denied the outpatient CR program.

Results:
After 6 months, VO2max was increased from 28.9 ± 5.79 to 31.7 ± 6.5 mL/kg/min (p < 0.001) and baPWV was decreased from 14.68 ± 2.66 to 13.54 ± 2.92 m/sec (p < 0.001). Changes of baPWV (Delta-baPWV) showed a significant negative correlation with 6-month VO2max (r = -0.339, p = 0.006). Patient were divided into group 1 (n = 32, Delta-baPWV <-1.13 m/sec) and group 2 (n = 33, Delta-baPWV > = -1.13 m/sec). Group 1 showed higher 6-month VO2max (33.5 ± 6.7 vs. 29.9 ± 5.9 mL/kg/min; p = 0.023). On logistic regression analysis, 6-month VO2max was a significant determining factor for Delta-baPWV (OR 1.159, 95% CI 1.030–1.305, p = 0.014) after adjustment of age, sex, height and baseline baPWV.

Conclusions:
Patients with better exercise capacity after CR program showed more decreased PWV.
PS-BPC02-6: ASSOCIATION BETWEEN MAJOR ADVERSE CARDIOVASCULAR EVENTS AND GENSINI SCORE IN HYPERTENSIVE PATIENTS WHO UNDERWENT CORONARY COMPUTED TOMOGRAPHY ANGIOGRAPHY
Authors: Miura Shinichiro , Miura Erika , Higashi Sara , Tashiro Kohei , Kawahira Yuto , Shiga Yuhei 
Published: January 2023

Objective:
From the Fukuoka University coronary computed tomography angiography (FU-CCTA) registry, we present major cardiovascular events (MACEs) in hypertensive patients who underwent CCTA, and the association between MACE and Gensini score of coronary arteries.

Design and method:
Of the patients who underwent CCTA for the purpose of screening for CAD at Fukuoka University Hospital (FU-CCTA registry), 318 hypertensive patients with suspected CAD or at least one cardiovascular risk factor were enrolled. A severity of atherosclerosis of coronary arteries was assessed by the Gensini score. The coronary artery calcification score (CACS) was defined as the presence of two or more contiguous pixels with more than 130 Hounsfield Units on CT images at the time of CCTA. MACEs (all-cause death, ischemic stroke, acute myocardial infarction, coronary revascularization) was a primary endpoint for the follow-up of patients for up to 5 years. The patients were divided into two groups according to the presence (MACEs group) or absence (non-MACEs group) of MACEs.

Results:
The average age was 68 ± 10 years and 50 % were males. The percentages of smoking, dyslipidemia, diabetes and chronic kidney disease were 37 %, 70 %, 26 % and 35 %, respectively. The %males, %smoking, CACS and Gensini score in the MACEs group were significantly higher than that in the non-MACEs group, whereas there were no differences in age, dyslipidemia, diabetes and chronic kidney disease between the groups. Regarding the relationships between MACEs and various coronary risk factors, CACS or Gensini score by a Cox regression analysis, significant relationships were observed for the CACS (p = 0.019) and the Gensini score (p = 0.002).

Conclusions:
CACS or Gensini score could be a predictor of MACEs in hypertensive patients who underwent CCTA.
PS-BPC04-2: ASSOCIATION OF SHORT-TERM BLOOD PRESSURE VARIABILITY AND MICROALBUMINURIA IN ESSENTIAL HYPERTENSION: DIFFERENCES ACCORDING TO CLASSIFICATIONS OF BLOOD PRESSURE ABSOLUTE VALUE
Authors: Koike Tsutomu , Tomoda Fumihiro , Ohara Maiko , Yamazaki Hidenori , Kinugawa Koichiro 
Published: January 2023

Objective:
Increased short-term blood pressure (BP) variability has been highlighted as a novel risk for cardiovascular diseases. The influence of short-term BP variability on cardiovascular mortality is also accelerated together with increase in BP. In essential hypertension, short-term BP variability is independently associated with early renal abnormalities. In this study, the associations of short-term BP variability with microalbuminuria were analyzed according to classifications of BP absolute value in essential hypertensive patients.

Design and method:
In 222 untreated essential hypertensive patients (127 males, average age 55 ± 11 years) without chronic kidney disease (urinary albumin excretion less than 300 mg/g of creatinine and estimated glomerular filtration rate 60 mL/min/1.73m2 or more), ambulatory BP monitoring was performed to estimate BP and the standard deviation of systolic BP (SBP-SD) during 24 hours. The 24-hour urine collection was also done to evaluate urinary albumin excretion. According to 24-hour BP level, patients were divided into 4 groups: 33 patients with BP < 130/80 mmHg (N), 71 patients with BP of 130–149/80–89 mmHg (G1), 84 patients with BP of 150–169/90–99 mmHg (G2) and 34 patients with BP 170/100 mmHg (G3).

Results:
24-hour systolic BP correlated positively with urinary albumin excretion in G1, G2 and G3 groups (r = 0.25 in G1 group; r = 0.22 in G2 group; r = 0.30 in G3 group, p < 0.05, respectively), whereas there was no relation between them in N group. SBP-SD correlated positively with UAE in both G2 and G3 group (r = 0.20 in G2 group; r = 0.30 in G3 group, p < 0.05, respectively), but did not so in either N or G1 group. Thus, the correlation between SBP-SD and urinary albumin excretion was confined to patients with 24-hour BP ≧ 150/90 mmHg. Furthermore, in patients with 24-hour BP ≧ 150/90 mmHg, multiple regression analysis revealed that SBP-SD was an independent determinant for urinary albumin excretion together with 24-hour systolic BP.

Conclusions:
These data indicate in essential hypertensive patients, that the risk of increased short-term BP variability for microalbuminuria could be potentiated along with the elevation in BP absolute value.
PS-BPC05-10: DIASTOLIC DYSFUNCTION, HYPERTROPHY AND HYPERTENSION VENTRICULAR-ARTERIAL UNCOUPLING TREATMENT
Authors: Piskorz Daniel , Keller Luis , Tissera Gabriel , Mata Lucrecia , Citta Luciano , Citta Paula , Bongarzoni Laureano 
Published: January 2023

Objective:
the aim of this study was to evaluate the effects of hypertension ventricular-arterial uncoupling treatment on left ventricular hypertrophy and diastolic dysfunction regression.

Design and method:
observational prospective study, consecutive hypertension patients. Left ventricle mass index measured by Devereux 2D echocardiography method and diastolic function by conventional and tissue Doppler following the Guidelines from Echocardiography from the American Society of Echocardiography and the European Association of Cardiovascular Imaging. Left ventricular end systolic elastance (Ees) was measured by Guarracino calculator, the effective arterial elastance (Ea) and ventricular-arterial coupling (VAC) measured by Sunagawa et al single beat method adapted by Chen et al in human ventricles. The sample was analyzed in quartiles (Q) according to VAC (Q1 worst VAC to Q4 best VAC). Follow-up 2 years.

Results:
288 patients, mean age 56.3 + 12.5 years and 168 patients (58.3%) males. VAC increased from 0.303 + 0.07 to 0.54 + 0.25 (p < 0.005) in Q1 mainly due to a reduction in Ees from 5.25 + 2.3 to 3.68 + 0.25 mm Hg/ml (p < 0.01) while Ea increased from 1.5 + 0.53 to 1.64 + 0.56 mm Hg/ml (p = NS); on the other side VAC was reduced from 0.87 + 0.15 to 0.68 + 0.26 (p < 0.05) in Q 4 with an increase in Ees from 2.28 + 0.6 to 2.67 + 1.07 mm Hg/ml (p = NS) and a reduction of Ea from 1.94 + 0.41 to 1.69 + 0.67 mm Hg/ml (p = NS) (Figure). The frequency of LVH was reduced from 31.9% to 10.8% in Q1 (p < 0.025) while moved from 14.7 to 17.9% (p = NS) in Q2, 22.9% to 13.9% (p = NS) in Q3 and 11.3 % to 23.7% (p = NS) in Q4. The frequency of normal diastolic function increased from 75% to 94.6% (p < 0.01) in Q1, from 78.7% to 100% in Q2 (p < 0.005), from 87.1% to 100% (p < 0.025) in Q3 and from 88.7% to 100% (0,025) in Q4.

Conclusions:
1) patients with the worst ventricular-arterial uncoupling (Q1) are the most VAC benefited from hypertension treatment; 2) regression of left ventricular hypertrophy was observed only in the group of patients with the worst ventricular-arterial uncoupling (Q1); 3) improvement in diastolic function was demonstrated in all Q of ventricular-arterial coupling. This study shows that achieving an optimal mechanical efficiency measured as ventricular-arterial coupling by hypertension treatment is essential for left ventricular hypertrophy and diastolic dysfunction regression.
PS-BPC06-5: EXOGENOUS THYROXINE INCREASES GLUT4 TRANSLOCATION IN MEMBRANE AND IMPROVES CARDIAC METABOLISM INDEPENDENT OF REDUCED SBP IN INSULIN RESISTANT OLETF RATS
Authors: Mendez Dora Alicia , Organis Jose Guadalupe Sonanez , Anaya Guillermo Vasquez , Nakano Daisuke , Nishiyama Akira , Ortiz Rudy Martin 
Published: January 2023

Objective:
During insulin resistance the heart undergoes a metabolic shift where fatty acids (FA) account for about 99% of the ATP production, indicative of impaired glucose metabolism. This results in increased reactive oxygen species and lipotoxicity leading to impaired mitochondrial function. Thyroid disorders such as hyperthyroidism and hypothyroidism both have the potential to lead to hypertension. Thyroid hormones (TH) may improve glucose intolerance by increasing glucose reabsorption and metabolism and therefore improve cardiac function and lower the risk of developing hypertension. However, incongruent data exists showing that TH promote oxidative stress and cardiac hypertrophy. Thus, the beneficial effects of TH in the heart during insulin resistance remains controversial. The glucose transport protein, GLUT4, is a downstream target of the TH response element indicating a possible mechanism by which TH regulate glucose metabolism.

Design:
Insulin resistant, Otsuka Long Evans Tokushima Fatty (OLETF) rats were used to assess the effects of exogenous thyroxine (T4) on glucose metabolism in cardiac tissue. Rats were assigned to four groups: 1) lean, Long Evans Tokushima Otsuka (LETO; n = 6), 2) LETO + T4 (8 μg/100 g BM/d × 5 wks; n = 7), 3) untreated OLETF (n = 6), and 4) OLETF + T4 (n = 7). T4 was administered via osmotic minipumps implanted subcutaneously. Systolic blood pressure (SBP) was measured weekly in conscious rats by tail-cuff plethysmography. Animals were provided water and food ad libitum.

Results:
SBP increased by 15% in OLETF compared to lean LETO (138 ± 2 vs. 117 ± 1 mm Hg), but T4 did not alter SBP is OLETF strain. T4 increased GLUT4 gene expression by 85% and GLUT4 protein translocation to the membrane by 328% in OLETF. Additionally, T4 increased phosphofructokinase-1, the rate limiting step in glycolysis, by 98% and decreased CD36 gene expression for FA transport by 20% in OLETF. We expect our recent metabolomics and lipidomics data to help better explain these molecular changes.

Conclusions:
Five-week dose of exogenous T4 did not reduce SBP is OLETF. The results suggest that increased T4 has the potential to increase glucose reabsorption and metabolism in the heart of insulin resistant rats and restore impaired substrate metabolism, but these improvements in substrate metabolism do not translate to sustained reduction in arterial pressure.
PS-BPC06-9: THE EFFECT OF COMMON SURGICAL MASKS ON UNATTENDED BLOOD PRESSURE CHANGES IN DRUG TREATED HYPERTENSIVE PATIENTS
Authors: Polyzos Dimitris , Drogkaris Sotiris , Tatakis Fotis , Manta Eleni , Fragkoulis Christos , Siafi Eirini , Filippou Christina , Kariori Maria , Chalmoukou Konstantina , Konstantinidis Dimitris , Thomopoulos Costas , Tsioufis Konstantinos 
Published: January 2023

Objective:
While there are several studies that have focused on the role of face masks in preventing airborne transmission of SARS-CoV-2, few data are available on their effects on physiological measures, and no study has examined their effects on blood pressure (BP). The purpose of our study was to investigate the effect of surgical masks on BP in drug-treated hypertensive patients who had a routine follow-up visit to a university hospital outpatient hypertension clinic.

Design and method:
The study included already treated hypertensive patients aged > 18 years, while the exclusion criteria were atrial fibrillation or any other arrhythmia affecting the BP measurement, an arm circumference > 42 cm, mental disorders, Parkinson disease, pregnancy, intolerance to the BP measurement method, or unwillingness to participate. A new surgical mask was provided to all participants to replace the face mask that was already in use. After the routine mask-on office BP measurement, patients were left alone and randomized to automated office BP measurement, with measurements taken after first wearing a mask for 10 min, then without wearing the mask for 10 min, and vice versa.

Results:
A total number of 265 patients were included in the study. Among the participants, 115 were women (43.4%), the mean age was 62 ± 12 years, and the mean office BP was 134 ± 15 / 81 ± 12 mmHg. There was no significant difference between mask-on unattended systolic BP (133 ± 15 mmHg) and mask-off unattended systolic BP (132 ± 15 mmHg) (P = 0.13) or between mask-on unattended diastolic BP (77 ± 13 mmHg) and mask-off unattended diastolic BP (76 ± 13 mmHg) (P = 0.32). Moreover, there was no difference in the heart rate (mask-on first, 69 ± 11 bpm; mask-off first, 69 ± 11 bpm, P = 0.7).

Conclusions:
Common surgical masks do not affect systolic/diastolic BP levels during unattended BP measurements in treated hypertensive patients.
PS-BPC07-1: PREEXISTING DIURETIC THERAPY DOES NOT ATTENUATE THE ANTIHYPERTENSIVE EFFECT OF EMPAGLIFLOZIN IN TYPE 2 DIABETIC PATIENTS WITH UNCONTROLLED HYPERTENSION
Authors: Kuzior Agnieszka , Hernandez-Lazaro Alba , Leon-Durango Ricardo de , Rios-Gomez Carlos , Santana-Ojeda Borja , Perez-Rivero Jennifer Maria , Perdomo-Herrera Esperanza , Tocino-Hernandez Alba Lucia , Gonzalez-Diaz Paula , Arnas-Leon Claudia , Acosta-Calero Carmen , Martinez-Martin Francisco Javier 
Published: January 2023

Objective:
The antihypertensive effect of SGLT2 inhibitors has been mostly overlooked, but in patients with type 2 diabetes mellitus (T2DM) and uncontrolled hypertension empagliflozin elicits a powerful systolic blood pressure (SBP) lowering effect, often attributed to diuretic/natriuretic effect and/or weight loss (WL), but other mechanisms are possible. We sought to retrospectively study the impact of the initiation of empagliflozin therapy on SBP in patients living with T2DM and uncontrolled hypertension in a real-world setting, and to assess its relationship with previous diuretic and other classes of antihypertensive therapy, and with weight loss during treatment.

Design and Methods:
Retrospective analysis of the SBP, weight and antihypertensive medication data in patients with T2DM who initiated empagliflozin therapy while having sitting office SBP > 140 mmHg. Mediation analyses with WL as mediator were performed post-hoc to quantify the relative contribution of WL to SBP reduction. Consent was obtained from all included subjects. Patients who changed their antihypertensive medication simultaneously with empagliflozin initiation were excluded.

Results:
Data were obtained from 198 patients (59.6% female, age 63.2 ± 8.9 years) from the visit in which empagliflozin (largely 10 mg/day) was started and the following one (3–4 months afterwards). Baseline BMI was 31.7 ± 8.5 Kg/m2. Baseline SBP was 166 ± 17 mmHg. 112 patients had ACEI/ARB, 76 diuretic, 69 calcium antagonist, 32 alpha-blocker and 29 beta-blocker therapy. The SBP reduction was 7.2 ± 5.7 mmHg (p = 0.012, paired t-test). The Figure shows comparisons by number and classes of antihypertensive drugs, with no significant differences for SBP reduction. The WL was 3.2 ± 1.7 kg, and it correlated significantly with the SBP reduction (Pearson's r: 0.22, p = 0.0012). The mediation analyses showed that a SBP reduction of 1.5 ± 0.9 mmHg was mediated by WL while 5.7 ± 4.9 mmHg was WL-independent.

Conclusions:
Empagliflozin initiation elicited a clinically relevant SBP decrease in patients with T2DM and uncontrolled hypertension. The number and classes of previous antihypertensive effects did not modify the SBP reduction. In particular, previous diuretic treatment does not attenuate it, suggesting that the diuretic effect is not the main driver of SBP reduction with empagliflozin. Also, the contribution of WL seems modest, implying alternative mechanisms for SBP reductions with empagliflozin; improvement of endothelial function and/or arterial stiffness and many others have been suggested.
PS-BPC10-2: A NOVEL PREDICTION MODEL OF ACUTE KIDNEY INJURY BASED ON COMBINED BLOOD VARIABLES IN STEMI
Authors: Goriki Yuhei , Tanaka Atsushi , Node Koichi 
Published: January 2023

Background:
Development of acute kidney injury (AKI) is associated with poor prognosis in patients with ST-segment elevation myocardial infarction (STEMI).

Objective:
We sought to investigate whether a combination of pre-procedural blood tests could predict the incidence of AKI in patients with STEMI.

Methods:
A total of 908 consecutive Japanese patients with STEMI who underwent primary percutaneous coronary intervention within 48 hours of symptom onset were recruited and divided into derivation (n = 617) and validation (n = 291) cohorts. A risk-score model was created based on a combination of parameters assessed on routine blood tests on admission.

Results:
In the derivation cohort, multivariate analysis showed that the following 4 variables were significantly associated with AKI: blood sugar ≧ 200 mg/dL (odds ratio [OR] 2.07), high-sensitive troponin I > 1.6 ng/mL (upper limit of normal × 50) (OR 2.43), albumin ≧ 3.5 mg/dL (OR 2.85) and estimated glomerular filtration rate < 45 mL/min/1.73 m2 (OR 2.64) (Table 1). Zero to four points were given according to the number of those factors. Incremental risk scores were significantly associated with a higher incidence of AKI in both cohorts (p < 0.001) (Figure 1). Receiver operating characteristic curve analysis of risk models showed adequate discrimination between patients with and without AKI (derivation cohort: area under the curve [AUC] 0.754, 95% confidence interval [CI] 0.733 - 0.846; validation cohort: AUC 0.754, 95% CI 0.644 - 0.839) (Figure 2).

Conclusions:
Our novel laboratory-based model might be useful for early prediction of the post-procedural risk of AKI in patients with STEMI.