bluuebunny/crossref_metadata_2025_split huggingface dataset:
Estrategias de determinación en oraciones inespecíficas en el español ecuatoriano
Authors: Favaro Federica , Rodríguez Hurtado Carlos Patricio , López Pérez Sonia de los Angeles , Orbea Peñafiel Mónica Narciza 
Published: June 2023

Introducción. Las oraciones inespecíficas introducen un Sintagma Nominal (SN) interpretado como no específico o un hecho interpretado como habitual. En esta tipología de oraciones, el español permite diferentes estrategias de determinación del objeto directo como el nombre escueto, el artículo indefinido y, aunque limitadamente, el artículo definido. Objetivo. Observar las preferencias de los hablantes español ecuatoriano lengua materna en cuanto a la determinación de los objetos directos de las oraciones inespecíficas. Metodología. Se ha creado un cuestionario de opción múltiple compuesto por 32 oraciones que sugerían una lectura inespecífica del objeto directo. Por cada oración se pusieron como opciones a elegir diferentes estrategias de determinación como artículo definido, artículo indefinido y nombre escueto. Los 96 participantes encuestados tenían que seleccionar la tipología de determinación que consideraban más adecuada. Resultados. De los datos recolectados se ha observado un amplio uso del artículo indefinido a cuesta del nombre escueto y un uso del artículo definido en estadio embrionario. Conclusiones. Del análisis de los resultados se puede concluir que, aunque el artículo definido español se coloque en la etapa II del ciclo de Greenberg, éste presenta un leve movimiento hacia la etapa III, representado por una limitada gramaticalización en el dominio de la inespecificidad, sobre todo en las oraciones genéricas interpretadas como habituales. Área de estudio general: Educación. Área de estudio específica: Lingüística.
Correlation between Clinical, Operative and Histopathological Findings of Adnexal Masses – A Study of 50 Cases
Authors: Rahman Dilara , Jahan Afia Akhter , Akter Asma , Parvin Mossammat Tahmina , Mile Jasrin Akter , Rouf Salma 
Published: June 2023

Background: Adnexal mass has multidimensional diagnosis that may be related to fallopian tube, ovary etc. These masses may be benign, intermediate and malignant. The ability of clinical approach to diagnose the exact pathology of adnexal mass is still a question.
Objective: To explore the correlation of clinical findings with operative and histopathological findings of adnexal masses.
Method and Materials: A prospective and observational study was conducted in a tertiary hospital during the period of July 2015 to December 2015. 69 patients were selected according to purposive sampling and further scrutinized according to selection criteria, which were clinically diagnosed as adnexal mass. All the 50 patients undergone surgical intervention followed by histopathology. All the patients’ data were recorded through a semi-structured data collection sheet.
Result: Study shows clinical and post-operative (per-operative + histopathology) findings of adnexal masses were 54% vs 50% for ovarian endometrioma, 24% vs 22% for benign ovarian tumor and 22% vs 26% for other adnexal masses respectively.
Conclusion: Clinical findings with the help of sonography can guide effectively to the diagnosis of adnexal masses. However, per-operative and histopathological findings are required for the confirmation.
Central Medical College Journal Vol 6 No 2 Jauly 2022 Page: 62-66
Pattern and seasonal variations of snake bite in southern part of Bangladesh
Authors: Uddin Mohammad Nazim , Ghose Aniruddha , Majumder Md Mahabubul Islam , Akter Raushan , Ali Mohammad Moharam , Ahmed Mostaque 
Published: June 2023

Background: Snake bite is one of the leading causes of mortality and morbidity in many regions, notably the rural tropics, and is a globally overlooked occurrence. Present study is aimed to find the pattern and seasonal variations of snakebite in the southern part of Bangladesh.
Methods: A one-year retrospective survey was done on 554 venomous or non-venomous snake bite patients admitting in a tertiary care hospital Chittagong Medical College Hospital, Bangladesh. Through the administration of a questionnaire, data on snake bites and kinds, date and time of bite and envenomation characteristics were obtained from respondents over a twelve-month period. After data collection, SPSS-20 was utilized for analysis.
Results: In the present study male (64.1%) were found more affected than female and the vast majority of snake bite victims are younger Regarding pattern of envenomation, 400(72.2%) patients had no features of envenomation, 37(6.7%) local envenomation with spontaneous bleeding, 35(6.3%) had systemic envenomation, 74(13.4%) had local envenomation and 8(1.4%) had local swelling with neurotoxicity. Most of the patients were male (64.1%), female was mostly housewives (24.9%) and 68.1% were from poor socioeconomic status. Regarding monthly distribution of study patients where most patients were found during May to September and venomous bite were also more common during the month of May to August. Analysis of species of snakes revealed 89(16.1%) were pit viper, 87(15.7%) were dora snake, 23(4.2%) were cobra and 355(64.1%) snake bite were remained unidentified.
Conclusions: During the monsoon season (May to September) in Bangladesh, the incidence of both nonvenomous and venomous snake bites is much high and poisonous snake bites are prevalent during this time. Poor access to health services and superstition regarding dealing with Ozahs are also a common finding in our study.
Central Medical College Journal Vol 6 No 2 Jauly 2022 Page: 71-76
MAHREM ALANDA SİVİL HUKUK İNŞASI: MAHMUT ESAT BOZKURT’UN MEDENİ KANUN GEREKÇESİ VE EUGEN HUBER KAYNAKÇASI
Authors: DUMANLI Çiğdem 
Published: June 2023

Cumhuriyet’in ilanından sonra bir dizi inkılâp gerçekleştiren kadrolar, bu bağlamda o ana kadar mevcut olan Mecelle yerine doğrudan devletin çekirdeği, aileyi ilgilendiren hukuki meselelerde de bir dönüşüm öngörmüşlerdir. Buna yönelik çalışmalar 1924 yılında başlamış, 1926 yılında İsviçre Medeni Kanunu örneğinde Türk Kanunu Medenisi’ni resepsiyon yöntemiyle kabul etmişlerdir.
Bu Kanun, Osmanlı döneminde Mecelle’nin düzenlemeye dâhil etmediği, kişi, aile ve miras hukukunu da kapsayacak bir temel oluşturmuş ve o ana kadar mahrem olan alanda sivil bir hukuk anlayışını ortaya koymuştur. Şüphesiz Medeni Kanun o günden bu güne birçok çalışmaya konu olmuştur. Hristiyan-Batı’dan alınma bir kanunun Müslüman-Doğu coğrafyasından bir ülke ve toplumda uygulanabilirliği, bunda ne kadar başarılı olunabileceği, eksiklikleri ve doğruları ile 1926 Medeni Kanunu günümüz tartışmalarında da güncelliğini korumaktadır.
Bu makalede, Osmanlı döneminde Mecelle’nin ortaya koyduğu sorunlara, yeni Kanun’un Meclise taşınma sürecine, Meclise geldiği noktada dile getirilen görüşlere değinilecektir. Bununla birlikte, bilhassa Adalet Vekili Mahmut Esat (Bozkurt)’un Kanun’a sunduğu gerekçe irdelenecektir. Söz konusu gerekçe, İsviçre Federal Arşivi (Schweizerisches Bundesarchiv) evrakları içerisinde bulunan, Eugen Huber’in bir seçki olarak sunduğu ve o dönem İsviçre’de mevcut hukuk sorunlarına ilişkin bilgiler ihtiva eden kaynakça ile karşılaştırılacaktır. Bu bağlamda hem dönemin hem günümüzün birincil kaynakları ve ikincil literatürü çalışmaya dahil edilecektir.
Effect of oligonucleotide MT01 delivered by N-isopropylacrylamide modified polyethyleneimine for bone regeneration
Authors: Zhang Qian , Qu Xingyuan , Liang Chen , Li Hongyan , Du Siyu , Wang Chang , Xie Yuandong , Zheng Yi , Wang Lei 
Published: June 2023

Objective: This study aimed to investigate the regulatory effect of N-isopropylacrylamide-modified polyethyleneimine (PEN)-delivered oligodeoxynucleotide (ODN) MT01 on bone regeneration in vitro and in vivo.Methods: A polyethylenimine (PEI) derivative, PEN, was constructed through Michael addition and employed as a carrier for ODN MT01 transfection. PEN/MT01 nanocomposites were characterized using agarose gel retardation assay, size distribution, zeta potential and transmission electron microscopy. The Cell Counting Kit-8 (CCK-8) assay was used to detect the effect of PEN on cell viability. Alkaline phosphatase (ALP) staining was used to detect the osteogenic differentiation ability of PEN/MT01 nanocomposite. Real-time quantitative PCR (q RT-PCR) and enzyme-linked immunosorbent assay (ELISA) were used to detect the regulatory effects of PEN/MT01 nanocomposite on osteogenic differentiation gene expression. Rat model was observed using the skull defect method and verified using micro-computed tomography (CT), serum biochemical indices, hematoxylin and eosin (H&amp;E) staining and Immunohistochemistry (IHC).Results: PEN had good biological properties and could deliver MT01 well to achieve efficient transmission of MT01. PEN/MT01 nanocomposites were effectively transfected into MC3T3-E1 cells at a ratio of 6.0. CCK-8 assay displayed that PEN had no cytotoxicity to MC3T3-E1 cells. Additionally, PEN/MT01 nanocomposites could promote the expression of osteogenic genes. In vivo results revealed that PEN/MT01 nanocomposites could promote bone regeneration more effectively than the other groups.Conclusion: PEN has good biocompatibility and low toxicity, which is a good carrier for ODN MT01. PEN-delivered MT01 can be potentially employed as a useful approach to achieving bone regeneration.
Identification of potential drug targets for varicose veins: a Mendelian randomization analysis
Authors: Lin Jianfeng , Zhou Jiawei , Liu Zhili , Zeng Rong , Wang Lei , Li Fangda , Cui Liqiang , Zheng Yuehong 
Published: June 2023

IntroductionVaricose veins are a common chronic disease that creates a significant economic burden on the healthcare system. Current treatment options, including pharmacological treatments, are not always effective, and there is a need for more targeted therapies. A Mendelian randomization (MR) method uses genetic variants as instrumental variables to estimate the causal effect of an exposure on an outcome, and it has been successful in identifying therapeutic targets in other diseases. However, few studies have used MR to explore potential protein drug targets for varicose veins.MethodsTo identify potential drug targets for varicose veins of lower extremities, we undertook a comprehensive screen of plasma protein with a two-sample MR method. We used recently reported cis-variants as genetic instruments of 2,004 plasma proteins, then applied MR to a recent meta-analysis of genome-wide association study on varicose veins (22,037 cases and 437,665 controls). Furthermore, pleiotropy detection, reverse causality testing, colocalization analysis, and external replication were utilized to strengthen the causal effects of prioritized proteins. Phenome-wide MR (PheW-MR) of the prioritized proteins for the risk of 525 diseases was conducted to screen potential side effects.ResultsWe identified eight plasma proteins that are significantly associated with the risk of varicose veins after Bonferroni correction (P &lt; 2.495 × 10−5), with five being protective (LUM, POSTN, RPN1, RSPO3, and VAT1) and three harmful (COLEC11, IRF3, and SARS2). Most identified proteins showed no pleiotropic effects except for COLLEC11. Bidirectional MR and MR Steiger testing excluded reverse causal relationship between varicose veins and prioritized proteins. The colocalization analysis indicated that COLEC11, IRF3, LUM, POSTN, RSPO3, and SARS2 shared the same causal variant with varicose veins. Finally, seven identified proteins replicated with alternative instruments except for VAT1. Furthermore, PheW-MR revealed that only IRF3 had potential harmful adverse side effects.ConclusionsWe identified eight potential causal proteins for varicose veins with MR. A comprehensive analysis indicated that IRF3, LUM, POSTN, RSPO3, and SARS2 might be potential drug targets for varicose veins.
Identify Tcea3 as a novel anti-cardiomyocyte hypertrophy gene involved in fatty acid oxidation and oxidative stress
Authors: Guo Yingying , Cen Xian-feng , Li Dan , Qiu Hong-liang , Chen Ya-jie , Zhang Meng , Huang Si-hui , Xia Hao , Xu Man 
Published: June 2023

BackgroundChronic pressure overload triggers pathological cardiac hypertrophy that eventually leads to heart failure. Effective biomarkers and therapeutic targets for heart failure remain to be defined. The aim of this study is to identify key genes associated with pathological cardiac hypertrophy by combining bioinformatics analyses with molecular biology experiments.MethodsComprehensive bioinformatics tools were used to screen genes related to pressure overload-induced cardiac hypertrophy. We identified differentially expressed genes (DEGs) by overlapping three Gene Expression Omnibus (GEO) datasets (GSE5500, GSE1621, and GSE36074). Correlation analysis and BioGPS online tool were used to detect the genes of interest. A mouse model of cardiac remodeling induced by transverse aortic constriction (TAC) was established to verify the expression of the interest gene during cardiac remodeling by RT-PCR and western blot. By using RNA interference technology, the effect of transcription elongation factor A3 (Tcea3) silencing on PE-induced hypertrophy of neonatal rat ventricular myocytes (NRVMs) was detected. Next, gene set enrichment analysis (GSEA) and the online tool ARCHS4 were used to predict the possible signaling pathways, and the fatty acid oxidation relevant pathways were enriched and then verified in NRVMs. Furthermore, the changes of long-chain fatty acid respiration in NRVMs were detected using the Seahorse XFe24 Analyzer. Finally, MitoSOX staining was used to detect the effect of Tcea3 on mitochondrial oxidative stress, and the contents of NADP(H) and GSH/GSSG were detected by relevant kits.ResultsA total of 95 DEGs were identified and Tcea3 was negatively correlated with Nppa, Nppb and Myh7. The expression level of Tcea3 was downregulated during cardiac remodeling both in vivo and in vitro. Knockdown of Tcea3 aggravated cardiomyocyte hypertrophy induced by PE in NRVMs. GSEA and online tool ARCHS4 predict Tcea3 involved in fatty acid oxidation (FAO). Subsequently, RT-PCR results showed that knockdown of Tcea3 up-regulated Ces1d and Pla2g5 mRNA expression levels. In PE induced cardiomyocyte hypertrophy, Tcea3 silencing results in decreased fatty acid utilization, decreased ATP synthesis and increased mitochondrial oxidative stress.ConclusionOur study identifies Tcea3 as a novel anti-cardiac remodeling target by regulating FAO and governing mitochondrial oxidative stress.
Efficacy and safety of single-pill amlodipine/losartan versus losartan in patients with inadequately controlled hypertension after losartan treatment: a multicenter, double-blind, randomized phase III clinical trial
Authors: Zhang Shuyang , Li Ying , Xu Xin , Xu Rui , Zhang Linchao , Wan Xiaoqun , Yao Zhuhua , Sun Yuemin , Liu Yong , Bin Jianping , Wang Zhen , Li Shuren , Yang Ping , Xu Xiping , Liang Weidong , Gao Xiaohong , Li Xiaodong , Jia Min , Ma Guang , Gu Xiang , Hong Chang 
Published: June 2023

ObjectiveSingle-pill amlodipine besylate (AML) plus losartan (LOS) has been used to treat inadequately controlled hypertension after antihypertensive monotherapy; however, relevant data in China are limited. This study aimed to compare the efficacy and safety of single-pill AML/LOS and LOS alone in Chinese patients with inadequately controlled hypertension after LOS treatment.MethodsIn this multicenter, double-blind, randomized, controlled phase III clinical trial, patients with inadequately controlled hypertension after 4 weeks of LOS treatment were randomized to receive daily single-pill AML/LOS (5/100 mg, AML/LOS group, N = 154) or LOS (100 mg, LOS group, N = 153) tablets for 8 weeks. At weeks 4 and 8 of treatment, sitting diastolic and systolic blood pressure (sitDBP and sitSBP, respectively) and the BP target achievement rate were assessed.ResultsAt week 8, the sitDBP change from baseline was greater in the AML/LOS group than in the LOS group (−8.84 ± 6.86 vs. −2.65 ± 7.62 mmHg, P &lt; 0.001). In addition, the AML/LOS group also showed greater sitDBP change from baseline to week 4 (−8.77 ± 6.60 vs. −2.99 ± 7.05 mmHg) and sitSBP change from baseline to week 4 (−12.54 ± 11.65 vs. −2.36 ± 10.33 mmHg) and 8 (−13.93 ± 10.90 vs. −2.38 ± 12.71 mmHg) (all P &lt; 0.001). Moreover, the BP target achievement rates at weeks 4 (57.1% vs. 25.3%, P &lt; 0.001) and 8 (58.4% vs. 28.1%, P &lt; 0.001) were higher in the AML/LOS group than those in the LOS group. Both treatments were safe and tolerable.ConclusionSingle-pill AML/LOS is superior to LOS monotherapy for controlling BP and is safe and well tolerated in Chinese patients with inadequately controlled hypertension after LOS treatment.
Eocene to Miocene paleoclimate reconstruction of the northern Tibetan Plateau: constraints from mineralogy, carbon and oxygen isotopes of lacustrine carbonates in the western Qaidam Basin
Authors: Li Shien , Liu Peixian , Guan Ping , Zhang Daowei , Xia Xiaomin , Ding Xiaonan , Zhang Chi , Zhang Jihua , Tang Jianzhou 
Published: June 2023

The Cenozoic climatic evolution of the Tibetan Plateau (TP), together with its driving mechanism, have been a subject of interest for decades. This study presents detailed sedimentology, mineralogical (XRD), carbon, and oxygen isotope analyses of lacustrine deposits from the Eocene to the Miocene in the western Qaidam Basin, the northern TP. The petrological observation and XRD data of 109 samples reveal that the sediments are composed of mixed siliciclastic, carbonate, and evaporate minerals. And the carbonate isotopic results show negative δ13C (−7.49‰ to −3.41‰) and negative to slightly positive δ18 values (−14.65‰ to 0.2‰). Both isotopes display a positive correlation with the contents of carbonates and evaporates, which suggests that evaporation is the major controlling factor of carbon and oxygen isotope. Therefore, the isotopes can be used as reliable indicators of the intensity of evaporation for paleoclimatic reconstruction. The reconstruction results reveal three distinct arid stages: top of the lower Xiaganchaigou Formation to the upper Xiaganchaigou Formation (ca.40-32 Ma), bottom of the Xiayoushashan Formation (ca.22-20 Ma), top of the Shangyoushashan Formation (ca.13-8.2 Ma). We suggest that the aridity during ∼40-32 Ma may have been related to the regression of the Paratethys Sea and uplift of the TP, while the aridity during 22-20 Ma may have been caused by the uplift and denudation of the mountains around the basin. The aridity after ∼13 Ma could be attributed to both global cooling and tectonic events in the northern TP. Furthermore, by comparing the climate records of the Qaidam Basin with those of other basins in Central Asia, a regional correlation can be established between different basins during the first and third drought stages. This study reveals that during the Eocene to Miocene, the climate change between different regions in the Qaidam Basin was synchronized and had a good connection with the surrounding basins, which responded to global climate change and regional tectonic activities.
Ovarian stimulation and oocyte cryopreservation in females and transgender males aged 18 years or less: a systematic review
Authors: Slonim Marnie , Peate Michelle , Merigan Kira , Lantsberg Daniel , Anderson Richard A. , Stern Kate , Gook Debra , Jayasinghe Yasmin 
Published: June 2023

BackgroundFertility preservation is an important healthcare focus in the paediatric and adolescent population when gonadotoxic treatments are required. Ovarian stimulation (OS) resulting in oocyte cryopreservation is a well-established fertility preservation option in the adult population. It’s utility, however, is little known in young patients. The purpose of this review was to synthesise the available literature on OS in patients ≤18 years old, to identify gaps in current research and provide suggestions for future research directions.MethodsUsing PRISMA guidelines, a systematic review of the literature was performed for all relevant full-text articles published in English in Medline, Embase, the Cochrane Library and Google Scholar databases. The search strategy used a combination of subject headings and generic terms related to the study topic and population. Two reviewers independently screened studies for eligibility, extracted data and assessed the risk of bias. Characteristics of the studies, objectives and key findings were extracted and summarised in a narrative synthesis.ResultsDatabase search and manual review identified 922 studies, 899 were eliminated based on defined exclusion criteria. Twenty-three studies were included and comprised 468 participants aged ≤18 years who underwent OS (median 15.2, range 7-18 years old). Only three patients were premenarchal, and four patients were on treatment to suppress puberty. Patients had OS for a broad range of indications including oncology treatment, transgender care and Turner syndrome. A total of 488 cycles of OS were completed, with all but 18 of these cycles (96.3%) successfully resulting in cryopreserved mature oocytes (median 10 oocytes, range 0-35). Fifty-three cycles (9.8%) were cancelled. Complications were rare (&lt;1%). One pregnancy was reported from a female who had OS aged 17 years old.ConclusionThis systematic review demonstrates that OS and oocyte cryopreservation is achievable in young females however there are only a few cases in the literature describing OS in premenarcheal children or those who have suppressed puberty. There is little proof that OS can lead to pregnancy in adolescents, and no proof that this can be achieved in premenarchal girls. Therefore it should be regarded as an innovative procedure for adolescents and experimental for premenarcheal girls.Systematic review registrationhttps://www.crd.york.ac.uk/prospero/display_record.php?RecordID=265705, identifier CRD42021265705.
Global drivers of timber carbon stock from income-based perspective
Authors: Wei Ying , Yi Meng , Yu Yuxin , You Yao , Zhang Weidi , Li Ruichao , Yu Chang , Wang Siyi 
Published: June 2023

Introduction: Timber and its products are key carriers of carbon stocks and can cause a hysteresis effect of carbon release in the carbon cycle of forest ecosystems. The literature regarding the cross-regional flow of timber carbon stock mainly pays attention to production- and consumption-based perspectives, which cannot reflect how the primary inputs drive timber carbon stock flow. The income-based perspective accounting can identify the influence of primary input suppliers and supplement research on timber carbon stock embodied in trade. The goal of this paper is to explore the cross-regional flow of global timber carbon stock and identify the critical countries from an income-based perspective.Methods: We used the Ghosh-multi-regional input-output (MRIO) model to calculate the income-based timber carbon stock flow among 190 countries. Furthermore, combined with the Leontief-MRIO model, a comparative analysis is carried out to analyze the different results of the income-based, production-based, and consumption-based methods.Results: The results showed that the income-based timber carbon stock of the United States and China were among the top countries in imports and exports simultaneously. However, their export volumes were significantly larger, meaning that these countries have invested more primary resources in timber products. The timber carbon stock of the United States mainly flows into Canada and Brazil. In China, the largest flow went to Canada. Furthermore, the flow to the United States increased significantly. Moreover, comparing the three perspectives shows that the United States’ primary inputs have a greater impact on the global timber production chain than their production- and consumption-based roles. Brazil and Russia, as main primary resource suppliers of timber carbon stock, are more important than as final consumers.Discussion: The research can contribute to clarify the flow of forest resources embodied in global trade activities. Furthermore, it also provides a scientific basis to fairly account for carbon offset shares to achieve better the goal of forest resource protection agreed upon in COP26.