bluuebunny/crossref_metadata_2025_split huggingface dataset:
A comparative analysis of current С-peptide assays compared to a reference method: can we overcome inertia to standardization?
Authors: Rohlfing Curt , Petroski Gregory , Connolly Shawn M. , Hanson Steven , Little Randie R. , Kabytaev Kuanysh 
Published: January 2025

Abstract

Objectives
C-peptide is an equimolar by-product of insulin biosynthesis. It is used clinically to assess insulin secretion and differentiate types of diabetes. However, the lack of standardization across assays limits its broader application. This study aimed to examine discrepancies between the leading C-peptide measurement methods used in clinical laboratories and propose a solution to reduce them based on a complete traceability chain.

Methods
Two sets of serum samples were distributed to 10 manufacturers of C-peptide assays. The first set (A, n=20) was analyzed independently by each manufacturer, who then returned their results to us. Subsequently, we sent out the second set (B, n=20) along with the reference values for set A. For set B, each manufacturer provided both non-calibrated and recalibrated values for each sample. The recalibration was performed according to each manufacturer’s internal standard protocols. We assessed how recalibration affected agreement between methods and alignment with the reference method. Non-parametric statistical approaches, including Passing-Bablok regression, level of agreement, and standard deviation analysis, were applied to compare data from multiple perspectives.

Results
Despite most manufacturers using the same WHO C-peptide calibrator material, significant disagreement was observed between methods prior to recalibration. Recalibration with matrix-appropriate serum samples reduced the discordance among assays, bringing them closer to the reference method. Overall, recalibration reduced both systematic bias and individual assay disagreement.

Conclusions
These findings underscore the importance of appropriate calibration schemes to improve agreement across C-peptide assays, enhancing the accuracy of C-peptide testing for clinical practice.
Positive answer to the invariant and hyperinvariant subspaces problems for hyponormal operators
Authors: Liu Junfeng , Mecheri Salah 
Published: January 2025

Abstract
There is a resent paper claiming that every hyponormal operator which is not a multiple of
the identity (operator) has a nontrivial hyperinvariant subspace. If this claim is true, then every hyponormal operator has a nontrivial invariant subspace, and every subnormal operator which is not multiple of the identity has a nontrivial
hyperinvariant subspace. But we find out that the proof of the above claim goes wrong.
Thus, the invariant subspace problem of the hyponormal operator and the hyperinvariant problem of subnormal
operator remain unresolved. Moreover, it is well known that these are two important research topics in operator
theory that have not been solved for a long time, and that many researchers have been trying to solve these two problems.
So, it is very meaningful to clarify whether these two problems have been solved.
Infinite system of integral equations associated with birth-and-death stochastic process: A challenge to solve
Authors: Banaś Józef , Madej Justyna , Rzepka Beata 
Published: January 2025

Abstract
The paper is dedicated to formulate an open problem concerning the existence of solutions of an infinite system of nonlinear differential (or integral) equations which are obtained during the modelling of the so-called birth-and-death stochastic process. Namely, creating a model of the mentioned birth-and-death process and also taking into account nonlinear components which are usually deleted during standard considerations, we obtain an infinite system of nonlinear differential (or integral) equations on a bounded or unbounded interval. It turns out that the operator associated with that infinite system is unbounded in all known classical Banach or Fréchet spaces. This fact initiates an open problem which will be described and raised in the paper.
Knockdown of CCNB1 alleviates high glucose-triggered trophoblast dysfunction during gestational diabetes via Wnt/β-catenin signaling pathway
Authors: Xiao Biru , Zhang Wenmiao , Ji Nini , Chen Qiuyue 
Published: January 2025

Abstract
Gestational diabetes mellitus (GDM), defined as glucose intolerance occurring or first detected during pregnancy, affects approximately 8% of pregnancies worldwide. The dysfunction of trophoblasts in pregnancies complicated by GDM is associated with changes in trophoblast cell functions, resulting in compromised proliferation and regulation of the cell cycle. Cyclin B1 (CCNB1), a pivotal controller of the start of mitosis, is crucial in these mechanisms. Nevertheless, the precise function of CCNB1 in trophoblast dysfunction related to GDM has not been extensively investigated. The aim of this study was to investigate CCNB1’s role in high glucose (HG)-triggered trophoblast. Herein, we revealed that in HG-stimulated HTR8/SVneo cells, CCNB1 is highly expressed. Knockdown of CCNB1 significantly promotes the growth of HG-stimulated HTR8/SVneo cells and suppresses inflammation (p < 0.05). Additionally, reducing CCNB1 expression significantly improves glucose uptake and inhibits the Wnt/β-catenin pathway in HG-stimulated HTR8/SVneo cells (p < 0.05). In conclusion, our study demonstrated that the deletion of CCNB1 can alleviate trophoblast dysfunction induced by HG in GDM through the Wnt/β-catenin pathway. This suggests that CCNB1 may be a potential target for managing GDM. Although our results underscore the potential therapeutic benefits of reducing CCNB1 in mitigating trophoblast dysfunction, it is important to note that the study is limited by its reliance on a single cell line and the absence of in vivo validation.
New alkaline earth and rare earth representatives adopting the Ce2Al16Pt9-type structure
Authors: Engel Stefan , Janka Oliver 
Published: January 2025

Abstract
The M
2Al16Pt9 series with M = Ca, La–Nd and Sm–Gd was synthesized from the elements in analogy to the literature known compounds Ce2Al16Pt9 and Sr2Al16Pt9. All compounds crystallize in the orthorhombic crystal system with space group Immm and have been characterized by powder X-ray diffraction. For La2Al16Pt9 and Eu2Al16Pt9 also single-crystal investigations have been conducted. In all cases, Al3Pt2 as well as the members of the REAl5Pt3 series (YNi5Si3 type, space group Pnma) have been observed as side phases. Annealing led in all cases to an increase of the side phases, suggesting that the title compounds decompose peritectically. In the crystal structures, polyanionic [Al16Pt9]
δ– networks can be found with large fourfold capped hexagonal prismatic cavities (M@Al6Pt6Al4), which host the respective M atoms. As seen from the unit cell volumes, the La–Nd, Sm and Gd compounds exhibit a formally trivalent oxidation state while the Ca and Eu atoms in Ca2Al16Pt9 and Eu2Al16Pt9 are divalent.
High-pressure synthesis and crystal structure of Al3BO6
Authors: Widmann Ingo , Oberndorfer Thomas , Huppertz Hubert 
Published: January 2025

Abstract
Orthorhombic Al3BO6 was synthesized in a Walker-type multianvil apparatus under high-pressure/high-temperature conditions of 5 GPa and 1,200 °C, respectively. Its structure has been determined via single-crystal diffraction and is isotypic to M
3BO6 (M = Cr, Fe) and homeotypic to the natural mineral norbergite (Mg3SiO4(OH)2). Thereby, we could complement the first report of Al3BO6 by Capponi et al. in 1972 (Capponi, J.-J.; Chenavas, J.; Joubert, J.-C. Eur. J. Mineral.
1972, 95, 412–417.) by providing a precise structure description. Al3BO6 crystallizes in the space group Pnma (no. 62) with 4 formula units per unit cell and the lattice parameters a = 9.5060(5), b = 8.1058(4) and c = 4.2899(2) Å. Infrared spectroscopy (powder sample) and high-temperature X-ray powder diffraction further characterize the compound.
LiEu[BO3]: a lithium europium(II) ortho-oxoborate with a familiar crystal structure
Authors: Hoslauer Jean-Louis , Reckeweg Olaf , Lissner Falk , Schulz Armin , Schleid Thomas 
Published: January 2025

Abstract
Yellow, transparent single crystals of LiEu[BO3] were obtained by serendipity in a solid-state reaction with the educts Eu2O3, MgBr2 and Li[BH4] at 750 °C within an arc-welded niobium ampoule enclosed in an evacuated fused silica secondary jacket. LiEu[BO3] crystallizes isotypically to LiBa[BO3] and LiSr[BO3] in the monoclinic space group P21/n exhibiting the unit cell parameters a = 638.79(4) pm, b = 677.12(4) pm and c = 690.43(4) pm with a monoclinic angle of β = 110.648(3)° for Z = 4. Li+ is coordinated by five oxygen atoms from three triangular planar [BO3]3– anions, while Eu2+ has seven oxygen neighbors belonging to six ortho-oxoborate groups. A brief synopsis of the crystal structures of other LiM
+II[BO3] compounds is given and the Raman spectrum of LiEu[BO3] compares well with the infrared spectrum obtained from the non-isotypic LiCa[BO3].
C–S–H–PCE nanocomposites as hydration accelerator in calcined clay-limestone-blended low carbon cement
Authors: Wang Xinyue , Plank Johann 
Published: January 2025

Abstract
Recently, providing admixtures to improve the performance of the novel low-carbon-blended cement is needed to meet the requirement for environmentally friendly cements. In this study, calcium-silicate-hydrate (C–S–H) nanocomposites which are known as hydration accelerator for Portland cement were employed in such low-carbon cements. The investigation focused on the effect of polycarboxylate ether (PCE) possessing different side-chain lengths on the morphology of C–S–H nanocomposites synthesized from sodium silicate and calcium nitrate via chemical co-precipitation and on their performance (especially early strength enhancement) when applied in a calcined clay-blended cement (OPC substitution rate of 46 wt%). As observed by TEM, addition of PCE effectively controls the size of the C–S–H nanoparticles and delays the transition from C–S–H globules to foils. PCEs with short side-chain (23 EO units) exhibited the highest inhibition effect on the growth of the C–S–H nuclei, which was confirmed by particle size measurements. Additionally, incorporating such C–S–H–PCE nanocomposites into the calcined clay-blended cement (dosage 2.0 % by weight of cement) significantly improved fluidity of the cement pastes. Regarding compressive strength, pristine C–S–H produced only a minor improvement, whereas C–S–H–PCE nanocomposites yielded substantial enhancement, especially after 16 h and 1 day of curing. Remarkably, C–S–H–23PCE pronounced the strongest seeding and strength enhancing effect, owed to its particularly small particle size (d = 21.8 ± 0.3 nm). It also significantly accelerated the hydration of the silicate phases (C3S and C2S) in this blended cement, as was evidenced by isothermal heat flow calorimetry and XRD measurements.
Homo- and heteronuclear complexes derived from N-picoline-functionalized benzimidazolin-2-ylidene ligands
Authors: Jahnke Mareike C. , Hahn F. Ekkehardt 
Published: January 2025

Abstract
The transformation of gold(I) complexes of the type [AuCl(NHC)] (NHC = N-picoline-N′-R-functionalized benzimidazolin-2-ylidene ligand; R = Et, 1; R = n-Pr, 2; R = n-Bu, 3) into polynuclear complexes was studied. Chloride abstraction from [AuCl(NHC)] by treatment with silver tetrafluoroborate yielded the digold complexes [Au2(1)2](BF4)2–[Au2(3)2](BF4)2, featuring two gold atoms coordinated by an NHC carbon atom from one NHC ligand and a picoline nitrogen atom from the second NHC ligand each. Treatment of the gold complexes [AuCl(NHC)] (NHC = 1−3) with dicarbene silver complexes of type [Ag(NHC)2][AgBr2] (NHC = 1−3) in the presence of AgBF4 proceeded under transmetalation of the NHC ligand to gold and formation of discrete heterodinuclear di-NHC complexes [AgAu(1)2](BF4)2−[AgAu(3)2](BF4)2, where the two NHC donors bind to the gold atoms, while the two pyridine donors bind to the silver atom. Reaction of the gold(I) complex [AuCl(4)] (4 = N,N′-dipicoline-functionalized benzimidazolin-2-ylidene) with silver(I) complex [Ag(4)2][AgBr2] in the presence of AgBF4 yielded the trinuclear heterobimetallic complex [Ag2Au(4)2](BF4)3 featuring the gold atom coordinated by two carbene donors and the two silver atoms coordinated by the two pyridine donors from one ligand 4. The molecular structures were determined for the homonuclear digold as well as for the heteronuclear AgI/AuI and AgI
2/AuI complexes.
“Coordination caps” of graded electron-donor capacity
Authors: Stritt Anika , Ünal E. Alper , Irran Elisabeth , Grohmann Andreas 
Published: January 2025

Abstract
An efficient synthesis of the novel {6-[1,1-di(pyridin-2-yl)ethyl]pyridine-2-yl}2-methyl-1,3-propanediamine (2) is reported, as well as a reliable large-scale synthesis (of the order of 100 g) of previously known 2,2’-[1-(6-chloropyridin-2-yl)ethane-1,1-diyl]dipyridine (4); the latter is the starting material for the preparation of the former, as well as a multitude of other polypodal polyamine/polyimine ligands. Both materials, as well as the intermediates in their multi-step syntheses, have been fully characterised. Ligand 2, in conjunction with ligands 2,2’-(pyridine-2,6-diyl)bis(2-methylpropane-1,3-diamine) (1) and 2,6-bis(1,1-di(pyridin-2-yl)ethyl)pyridine (3), establishes a series of tetrapodal pentadentate N5 ligands L of like scaffold, and thus coordination geometry, but graded primary amine/imine donor atom ratios. The iron(II) complexes [Fe(L)NCCH3](OTf)2 (L = 1: A; L = 2: B; L = 3: C; OTf = triflate) have been prepared and fully characterised, including X-ray single-crystal structure analyses. The metal-centred one-electron oxidation/reduction potential (FeII/FeIII) depends sensitively on the electron donor capacity of the capping ligand used, as demonstrated by cyclic voltammetry. Whereas the acetonitrile ligand in A and C is readily exchanged for a methanol ligand in methanol solution, the resulting complexes showing variable-temperature spin crossover (SCO) in solution, B has been found to be inert to this type of ligand exchange.
French marigold (Tagetes patula) flavonoid extract-based priming ameliorates initial drought stress on Oryza sativa var indica, cultivar Satabdi (IET4786): a sustainable approach to avoid initial drought stress
Authors: Saha Arunima , Kumar Suraj , Dey Sanjukta , Bhattacharyya Chiranjib , Sahu Ranabir , Ashraf Gouhar Jahan , Bhattacharyya Somnath , Das Pritam , Bandyopadhyay Subhendu , Dewanjee Saikat , Gangopadhyay Moumita 
Published: January 2025

Abstract
Drought stress remains a serious concern in Oryza sativa L. var indica, cultivar Satabdi (IET4786) production, particularly during the earliest growth phases, ultimately affecting yield due to the recent trend of delayed rain arrival in West Bengal, India. This study aimed to develop a cost-effective strategy to improve the drought tolerance capacity of rice seedlings by priming the seeds with flavonoid-enriched extract (FEE) of French marigold (Tagetes patula) petals to withstand the initial drought milieu. The morpho-physiological and biochemical responses of rice seedlings were evaluated to perceive the priming efficacy in alleviating water stress-induced untoward effects. The findings revealed that mechanical priming of the IET4786 seeds with FEE (50 mg/mL for 30 min) significantly improved seedling survival against initial drought stress for 14 days. After 24 h of recovery from drought stress, the primed seed-derived seedlings exhibited significantly improved morphological, physiological, biochemical, and redox parameters compared to the seedlings derived from unprimed seeds under net house conditions. In search of mechanistic insights, seed priming significantly increased proline content by endorsing Δ1-pyrroline-5-carboxylate synthetase activities, endorsed methylglyoxal clearance homeostasis by improving glyoxalase I and II activities through restoring glutathione (GSH) level, and enhanced polyamine accumulation in the leaves of seedlings to endure drought stress.
Optimizing Stereolithography Printing Parameters for Enhanced Microfluidic Chip Quality
Authors: El Biyari Nidal , Zekriti Mohssin 
Published: January 2025

Abstract
In the pursuit of innovative biosensing technologies for critical applications such as early breast cancer detection, the development of efficient and portable devices is crucial. This work describes a unique stereolithography (SLA)-based three-dimensional–printed microfluidic device intended particularly for optofluidic biosensing with just microliter quantities of blood, similar to diabetes monitoring devices. Unlike typical cumbersome lab equipment such as the Biacore machine, which needs large blood sample volumes and laboratory processing, microfluidic technology allows for patient-operated, at-home testing, decreasing the requirement for hospital visits. The main contribution of this study is to optimize the SLA printing parameters, namely the exposure duration, in order to improve the microfluidic chip’s transparency and channel quality. This improvement allows for the exact immobilization of biorecognition components within the channels, resulting in sensitive and efficient biomarker detection. By extending the exposure duration, we considerably increase the structural integrity and optical clarity of the microfluidic channels, which are critical for successful biosignal transduction in labeled sensing applications. This development not only leads to a cheaper cost and faster manufacturing compared with conventional technologies but also offers increased performance in real bio-sensing applications. Thus, our work represents a big step forward in the development of accessible, efficient, and compact devices for early-stage illness diagnosis, outperforming existing lab-based diagnostics.
S’unir pour accompagner les élèves dans le développement des compétences en lecture à l’ère du numérique
Authors: Legault France , Beaudry Marie-Christine , Carignan Isabelle 
Published: January 2025

L’utilisation du numérique en classe amène les élèves à lire sur différents supports. L’enseignement de stratégies de lecture adaptées apparait donc important pour pouvoir outiller les élèves. Au secondaire, une difficulté supplémentaire s’impose : le transfert des apprentissages entre les disciplines. Ces enseignements doivent également être accessibles aux parents afin que ces derniers puissent soutenir leur enfant. Actuellement, le personnel enseignant a peu de ressources pour soutenir le développement de ces stratégies de lecture (Legault et al., 2021). Ainsi, la mise en place de structures de communication et d’outils favorisant la littératie, autant à l’école qu’à la maison, semble être une avenue prometteuse.
Deconstructing the Gender Stereotypes of the Villainess in Dan Brown’s Inferno
Authors: Theodar Caleb 
Published: January 2025

This study intends to contribute to the existing research about modern female villains by delving deeper into the ways that represent the stereotypical pattern in film and literature. This article examines Sienna Brooks, the primary antagonist in Dan Brown’s novel Inferno and its film version. She was Bertrand Zobrist’s lover and resolved to finish his work by unleashing his Inferno virus on humanity. Vis-à-vis, articulating feminist momentum through Sienna’s antagonism to the hero highlights the boundaries of binary oppositions, challenging literary critics and questioning gender bias in literature. Female villains have empowered characters because they are often multidimensional, stronger, and more complex than female heroines, instrumental to the story’s narrative. Brown’s block characterisations make inferences about Sienna Brooks both explicitly and implicitly, replicating Sienna’s thinking style or how she thinks about the world in her mind. The conceptual portrayal of the feminine villain narrative reflects the evolving role of women in plot construction, highlighting the need for nuanced and equitable gender representation in movies.
Treatment Challenges in a Tourette Syndrome Patient with Self-Injurious Behaviour: Case Report and Literature Review
Authors: Asadauskaitė G. , Jasionytė G. , Karnickas M. 
Published: January 2025

Introduction. Tourette syndrome is known as a combined vocal and multiple motor tic disorder. It is a chronic complex neuropsychiatric disorder characterised by the onset of symptoms in early childhood and the presence of multiple motor tics and one or more vocal tics. In most cases, the symptoms resolve or become less severe in early adulthood. Nevertheless, there are some cases where the syndrome does not follow its typical clinical course.Case report. We present a 28-year-old patient with Tourette syndrome. The patient was hospitalized for complex motor and vocal tics, as well as tics resulting in self-harm. First tics began at the age of twelve and gradually intensified. The patient was diagnosed with Tourette syndrome at thirteen and has been taking Haloperidol 5 mg 3 times a day and Trihexyphenidyl 2 mg 3 times a day for the past 15 years. His condition worsened 6 months ago with increased tics, self-harm, insomnia, social withdrawal, and weight loss. In the hospital, Haloperidol was gradually replaced with 20 mg of Aripiprazole daily. The patient underwent twenty-six transcranial magnetic stimulation procedures. During hospitalization, insomnia was resolved, whereas motor tics and self-harm decreased.Discussion. The pathogenesis of Tourette syndrome emphasizes the dysfunction of neuronal networks in the basal ganglia. It leads to impaired inhibition in the sensorimotor cortex of the brain, which results in motor and vocal tics. Their characteristics are individual to each patient. The course of illness and the severity of symptoms are influenced by various stressors, intense emotional experiences, and environmental factors. Tourette syndrome is commonly treated with psychotherapy, pharmacological treatment, or a combination of these methods. The case we present shows that the conventional treatment methods are not always effective, and that the clinical course of Tourette syndrome may deviate from the usual expressions of the syndrome.
Cuidados de fim de vida prestados a cardiopatas: necessidade de maior integração com equipes de Cuidados Paliativos. Série de casos e revisão da literatura
Authors: Cristofolini Bruna , Quispe Julee , Tavares Laura , Coviello Carolina , Lisboa Brenda , Dei Santi Daniel , Tavares Ricardo 
Published: January 2025

ANTECEDENTES
Existe alta demanda por Cuidados Paliativos (CP) em pacientes com doenças cardiovasculares. Profissionais de qualquer especialidade devem ser capazes de oferecer Cuidados Paliativos e buscar ajuda destes especialistas quando necessário.
OBJETIVO
Avaliar a percepção de Cardiologistas (C) sobre a necessidade de CP para seus pacientes, se isso motivou interconsulta com equipe de CP e quais cuidados eles ofereceram para os pacientes que faleceram durante a internação.
MÉTODOS
Foram avaliados os casos internados, em um dia, num centro quaternário de cardiologia, e selecionados os casos que morreram na internação. Destes, foram coletados dados demográficos, clínicos, de intervenções realizadas durante a internação e de decisões de limitação de suporte terapêutico (LST). Os C foram questionados sobre o risco de óbito na internação e a necessidade de CP de seus pacientes.
RESULTADOS
Os C reconheceram que 82,35% dos pacientes estavam em fase final de vida. Afirmaram que 75,53% precisavam de CP, mas solicitaram interconsulta com o Paliativista para apenas 16% destes. 41,18% dos pacientes receberam plano de limitação de suporte, 76,5% morreu em Unidades de Terapia Intensiva, e foi observado um número importante de intervenções.
CONCLUSÃO
Os Cardiologistas reconheceram a necessidade de Cuidados Paliativos para a maior parte dos pacientes, mas também discutiram LST para poucos e indicaram intervenções potencialmente inadequadas. Mesmo reconhecendo essa necessidade, não convocaram a equipe de CP.