bluuebunny/crossref_metadata_2025_split huggingface dataset:
Ste20‐like kinase activity promotes meiotic resumption and spindle microtubule stability in mouse oocytes
Authors: Song Ke , Jiang Xiuying , Xu Xiangning , Chen Ye , Zhang Jiaqi , Tian Ying , Wang Qian , Weng Jing , Liang Yuanjing , Ma Wei 
Published: December 2022

AbstractSte20‐like kinase (SLK) is involved in cell proliferation and migration in somatic cells. This study aims to explore SLK expression and function in mouse oocyte meiosis. Western blot, immunofluorescence, Co‐immunoprecipitation, drug treatment, cRNA construct and in vitro transcription, microinjection of morpholino oilgo (MO) and cRNA were performed in oocytes. High and stable protein expression of SLK was detected in mouse oocyte meiosis, with dynamic distribution in the nucleus, chromosomes and spindle apparatus. SLK phosphorylation emerges around meiotic resumption and reaches a peak during metaphase I (MI) and metaphase II. SLK knockdown with MO or expression of kinase‐dead SLK K63R dramatically delays meiotic resumption due to sequentially suppressed phosphorylation of Polo‐like kinase 1 (Plk1) and cell division cycle 25C (CDC25C) and dephosphorylation of cyclin‐dependent kinase 1 (CDK1). SLK depletion promotes ubiquitination‐mediated degradation of paxillin, an antagonist to α‐tubulin deacetylation, and thus destroys spindle assembly and chromosome alignment; these phenotypes can be substantially rescued by exogenous expression of SLK kinase active fragment. Additionally, exogenous SLK effectively promotes meiotic progression and spindle assembly in aging oocytes with reduced SLK. Collectively, this study reveals SLK is required for meiotic resumption and spindle assembly in mouse oocyte meiosis.
A rare cause of pulmonary hypertension: Idiopathic fibrosing mediastinitis obstructing right pulmonary artery and pulmonary veins
Authors: Sonsoz Mehmet Rasih , Atamaner Oya , Inan Duygu , Kilicgedik Alev 
Published: December 2022

AbstractFibrosing mediastinitis is caused by a proliferation of fibrous tissue in the mediastinum with encasement of mediastinal viscera and compression of mediastinal bronchovascular structures. Pulmonary hypertension (PH) may occur as a severe complication of the disease. Herein, we report a patient diagnosed with fibrosing mediastinitis and pulmonary hypertension, whose imaging tests showed obstruction of right pulmonary artery and veins.
The influence of viral protein R amino acid substitutions on clinical outcomes in people living with HIV: A systematic review
Authors: Asia Levanco K. , Jansen Van Vuren Esmé , Williams Monray E. 
Published: December 2022

AbstractBackgroundThe HIV viral protein R (Vpr) is a multifunction protein involved in the pathophysiology of HIV‐1. Recent evidence has suggested that Vpr amino acid substitutions influence the pathophysiology of HIV‐1 and clinical outcomes in people living with HIV (PLWH). Several studies have linked Vpr amino acid substitutions to clinical outcomes in PLWH; however, there is no clear consensus as to which amino acids or amino acid substitutions are most important in the pathophysiology and clinical outcomes in PLWH. We, therefore, conducted a systematic review of studies investigating Vpr amino acid substitutions and clinical outcomes in PLWH.MethodsPubMed, Scopus and Web of Science databases were searched according to PRISMA guidelines using a search protocol designed specifically for this study.ResultsA total of 22 studies were included for data extraction, comprising 14 cross‐sectional and 8 longitudinal studies. Results indicated that Vpr amino acid substitutions were associated with specific clinical outcomes, including disease progressions, neurological outcomes and treatment status. Studies consistently showed that the Vpr substitution 63T was associated with slower disease progression, whereas 77H and 85P were associated with no significant contribution to disease progression.ConclusionsVpr‐specific amino acid substitutions may be contributors to clinical outcomes in PLWH, and future studies should consider investigating the Vpr amino acid substitutions highlighted in this review.
First report of Botrytis leaf blight caused by Botrytis cinerea on Aucuba japonica in China
Authors: Rui Lin , Kong Wei‐Liang , Sun Xiao‐Rui , Wu Xiao‐Qin 
Published: December 2022

AbstractIn May 2019, Aucuba japonica var. variegata, known as an evergreen shrub, was found to have multiple leaf spots in Nanjing City, Jiangsu Province, China. A fungus was isolated from the margins of the lesions and one isolate JS‐RL3 was selected for identification and pathogenicity tests. The morphological characteristics of the fungus were similar to Botrytis cinerea. Phylogenetic analysis using concatenated sequences of ITS and G3PDH also showed that isolate JS‐RL3 clustered with strains of B. cinerea, a relationship supported by a 100% bootstrap value. This report is the first of B. cinerea causing leaf spots on A. japonica var. variegata.
How a Government Organisation Evolved to Embrace Ethnographic Methods for Service (and Team) Resilience: The Case of the Canadian Digital Service
Authors: NAIK MITHULA , MACARTHUR COLIN 
Published: December 2022

Government websites and online services are often built with limited input from the people they serve. This approach limits their ability to respond to ever changing needs and contexts. This case study describes a government digital team built from the ground‐up to embrace ethnographic methods to make government services more resilient.The case study begins by tracing the organisation's origins and relationship to other research‐driven parts of its government. Then it shows how the organisation's structure evolved as more projects included ethnography. It describes various approaches to locating skilled researchers within bureaucratic confines, as well as what responsibilities researchers took on as the organisation grew. It then summarises researchers' experiences with matrixed, functional and hybrid organisation schemes.The case study concludes explaining how embracing ethnographic approaches (and values) increased not only online service, but also organisational resilience. Teams who embraced ethnography had deeper and more thoughtful responses to the pandemic, and inclusivity challenges in the organisation. Lessons learned for other organisations attempting to scale an ethnographic research practice, and seize its benefits for resilience.
Designing and Envisioning a More Resilient Social System: How to Start from What's Good to Create Something Better in Public Services
Authors: CARVALHO SOFIA 
Published: December 2022

Segurança Social (Portugal's Social Security System) offers multiple service channels to the people. However, they were not perceived as a whole because the assistance was not standard, depending on the channel or person answering—leading to cumulative problems that could take months to resolve.We faced the complex nature of a big governmental organization. Our research made us more aware of people's general reluctance towards public institutions as they tend to expect poor quality service. We used the information from field research to create four prototypes that would bring tangible results to citizens and impact the institution's culture in the long term.Segurança Social has always been about resilience: the organization itself and the people it serves. Despite its flaws and fragilities, it's the social system that allows many to thrive. That's why we envisioned the system's sustainability, rooted in the workers' resilience and processes.
Synergetic fusion of Reinforcement Learning, Grey Wolf, and Archimedes optimization algorithms for efficient health emergency response via unmanned aerial vehicle
Authors: Gupta Himanshu , Sreelakshmy K. , Verma Om Prakash , Sharma Tarun Kumar , Ahn Chang Wook , Goyal Kapil Kumar 
Published: December 2022

AbstractOwing to the recent technological innovations, unmanned aerial vehicles (UAVs) are progressively employed in various civil and military applications, including healthcare. This requires estimating an optimum route under various real‐world complexities, such as non‐uniform obstacles. However, most of the reported work considers only uniform obstacles as an object, which limit their practical applicability. Hence, Archimedes optimization algorithm (AOA) is examined to overcome this limitation. Further, it is observed that many a time, AOA over‐exploits the search space, resulting in higher computational time. Therefore, the present work fuses AOA with grey wolf optimizer (GWO) to improve the convergence capability. Also, reinforcement learning (RL) is employed to intelligently switch between the exploration and exploitation phases. The efficacy of the developed algorithm is statistically analysed and validated against various metaheuristics on several benchmark functions. The simulated results verified that the developed RLGA provides optimal or near‐optimal solutions more efficiently relative to other metaheuristics. Moreover, it also affirms the hypothesis that the proposed modifications significantly improve the convergence speed of AOA. Finally, the appropriateness of RLGA is tested and validated by rigorous experimentation on real‐world 3D‐route estimation problems for UAVs. The simulated results reveal that RLGA produces a flyable path with 51.46%, 62.06%, and 70.42% lesser cost than RLGWO, AOA, and GWO, respectively. This ensures the employability of RLGA for efficient medical assistance in minimum time‐, energy‐, and transportation‐cost with safe and smooth UAV auto‐navigation for developing drone doctors.
The application of digital image correlation (DIC) in fatigue experimentation: A review
Authors: Hebert John , Khonsari Michael 
Published: December 2022

AbstractIn recent years, the use of digital image correlation (DIC) in fatigue experiments has become widespread. It is estimated that ~1000 published works exist that outline fatigue experiments in which DIC is employed for displacement and strain measurement. Of these, ~900 were published in the last 10 years. DIC is a noncontact method that uses a series of digital images to calculate full‐field strains on the surface of an object, planer or curved. Typical commercial DIC systems compute strains at resolutions high enough to trace hysteresis loops in metals. Properly operated open‐source systems can do the same. The DIC method is applied not only on optically based digital images but also on digital images from ultra‐high resolution (ultra‐HR) microscopes like a scanning electron microscope (SEM) or on volumetric images from computed tomography (CT) scans. In fatigue analysis, DIC provides much more information than that of an extensometer. Full‐field strains from DIC can be acquired at different scales (i.e., microscale, macroscale, and nanoscale) and can be related to items such as microstructural features, interacting surfaces (e.g., fretting), fatigue crack growth phenomenon, and distinct forms of energy. Because fatigue is a highly complex, strain‐induced process, the DIC method is and will be an important tool for current and future research in fatigue. This review begins with an overview of the history and fundamentals of DIC including an evaluation of the overall performance and accuracy of the method. Publications selected for review are then presented and discussed. Remarks about the present state‐of‐the‐art and an outlook for future work to be done are then provided.
Estimates of niche position and breadth vary across spatial scales for native and alien inland fishes
Authors: Cano‐Barbacil Carlos , Radinger Johannes , Olden Julian D. , García‐Berthou Emili 
Published: December 2022

AbstractAimWe estimate and compare niche position, marginality and breadth of Iberian inland fishes at three geographical extents (regional, restricted to the species’ range and global) to understand the effect of spatial scale on niche metrics. Furthermore, we investigate differences in niche metrics between native and alien fish, and test for associations with introduction date of alien species and niche characterization to better understand their invasion process.LocationIberian Peninsula and global.Time period2000–2020.Major taxa studiedFifty‐one native and 17 alien inland fish species from the Iberian Peninsula.MethodsOutlying mean index (OMI) analyses were used to estimate the niche position, marginality and breadth of Iberian inland fishes. Climatic OMI analyses were computed at three different scales (regional, restricted to the species’ range and global). Permutational analyses of variance (PERMANOVAs) were used to test for differences in niche position, marginality and breath among native and alien species.ResultsNiche metrics differed depending on the geographical extent of the investigation, as well as with respect to species origin (native versus alien). Differences in climatic niche position between native and alien species observed at the global scale were non‐existent at the regional scale. The niche breadth of widely distributed alien species was highly underestimated when only considering the invaded region, and further influenced by the first date of of species introduction.Main conclusionsEstimating niches of freshwater species, especially of alien invaders, should carefully consider the geographical extent of the investigation. We suggest that analyses that jointly consider regional and global scales will improve the estimation of niche metrics of widely distributed organisms, particularly regarding species climatic niche, and the assessment of the invasive potential of species.
Current treatments and emerging therapies of human polyomavirus‐associated skin diseases: a comprehensive review
Authors: Bartley Brooke R. , Moore Stephen A. , Doan Hung Q. , Rady Peter L. , Tyring Stephen K. 
Published: December 2022

AbstractSince Merkel cell polyomavirus (MCPyV) was linked as the predominant etiology of Merkel cell carcinoma (MCC) in 2008, three additional human polyomaviruses (HPyV) have been definitively linked to cutaneous diseases—trichodysplasia spinulosa virus (TSPyV) and human polyomavirus 6 and 7 (HPyV6, HPyV7). TSPyV contributes to the development of trichodysplasia spinulosa (TS), and HPyV6/7 is associated closely with the eruption of pruritic and dyskeratotic dermatoses (PDD). Clinically, MCC is treated with surgical excision and radiation with adjuvant chemotherapy, although newer treatment options include immune checkpoint inhibition. These novel immunotherapies hold promise for the treatment of metastatic MCC, but resistance and side effects prevent a significant proportion of patients from realizing their benefits. Based on previous case reports, the standard of care for the less deadly but disfiguring cutaneous disease TS include immunosuppressant (IS) reduction, the use of antivirals such as cidofovir (CDV) or valganciclovir (VGCV), or a combination of these treatments. Similar treatments were attempted for PDD, but oral acitretin was found to be most effective. As MCC, TS, and PDD are rare diseases, further research is required for effective treatments. In this review, we summarize clinical trials, preclinical studies, and case reports that present outcomes and side effects of current and emerging treatments for HPyV‐associated cutaneous diseases, offering a comprehensive resource for clinical application and prospective clinical trials.
Physicochemical and sensory characteristics of pasta enriched with blue whiting (Micromesistius poutassou) fish protein hydrolysate
Authors: Khodaei Diako , Forde Aisling , Noci Francesco , Ryan Lisa 
Published: December 2022

SummaryBlue whiting fish protein hydrolysate (BWFPH) is a low‐value fish by‐product with potential health benefits. This study aimed to assess the effect of the addition of BWFPH (0%, 5%, 10% and 15% w/w) on the cooking quality, physical properties and sensorial characteristics of pasta. The results showed that by adding BWFPH to pasta, the optimal cooking time, swelling index, water absorption and cohesiveness decreased, while the cooking loss, total protein content, firmness and adhesiveness increased (P ≤ 0.05). The colour score and b* of pasta samples increased with the addition of BWFPH, while the lightness reduced. The samples containing 5% of BWFPH showed the highest sensorial parameters followed by 10% BWFPH, control and 15% of BWFPH. It can be concluded that fortification pasta with BWFPH has the potential for the food industry to deliver nutritionally enriched and high‐protein pasta.
High incidence of inflammatory bowel disease in Northern Australia: a prospective community population‐based Australian incidence study in the Mackay‐Isaac‐Whitsunday region
Authors: Flanagan Amanda , Allsopp Susan M. , O'Connor Sam A. , Tobin Jacinta , Pretorius Casper , Brown Ian S. , Bell Sally , Daveson A. James M. 
Published: December 2022

AbstractBackground and AimsTo determine the incidence of inflammatory bowel disease (IBD) in the Mackay‐Isaac‐Whitsunday region in Northern Queensland (−21.14° S) and to allow a comparison with Southern Australian and New Zealand data (Geelong, Australia −38.14° S; Tasmania −41.43° S and −42.88° S (Launceston and Hobart) and Canterbury, New Zealand −43.46 °S).DesignA prospective observational community population‐based IBD study was conducted between 1 June 2017 and 31 May 2018.Outcome measuresPrimary includes the crude annual incidence rate of IBD, Crohn's disease (CD), ulcerative colitis (UC) and inflammatory bowel disease‐unclassified (IBDU), while secondary includes disease phenotype and behaviour.ResultsFifty‐six new cases of IBD were identified. Twenty‐three were CD, 30 were UC and 3 were IBDU. The crude annual incidence rate per 100 000 for IBD, CD, UC and IBDU were 32.2 (95% confidence interval (CI): 24.78–41.84), 13.23 (95% CI: 8.79–19.90), 17.25 (95% CI: 12.06–24.67) and 1.73 (95% CI: 0.56–5.35). When directly age‐standardised to the World Health Organisation Standard Population Distribution, the overall CD, UC and IBDU incidence were 13.19, 17.34 and 1.85 per 100 000, with an overall age‐standardised IBD incidence of 32.38.ConclusionsThis is the first study to define the incidence of IBD in a Northern Australian cohort and to allow a comparison between North and Southern Australia. The IBD crude is the highest reported in Australia. Like others, we found a high and low incidence of upper gastrointestinal Crohn's disease and complicated disease at diagnosis respectively, likely reflective of the increased availability and early uptake of endoscopic procedures.
Capability bundling for effective supply chain management: An integrative framework and research agenda
Authors: Madhavaram Sreedhar , Manis Kerry T. , Rashidi‐Sabet Siavash , Taylor Daniel F. 
Published: December 2022

AbstractEffective supply chain management (SCM) capabilities are critical to the success of organizations. Although research over the past three decades (i) firmly establishes that SCM plays a significant role in corporate strategy, (ii) either suggests and/or finds strong positive results for the impact of different types of SCM capabilities on firm performance, and (iii) indicates that intra‐firm and inter‐firm capabilities are central to the effectiveness of SCM, how firms come to have the SCM capabilities stays somewhat underexplored. Therefore, to understand how firms can bundle effective SCM capabilities, we (i) develop a brief overview of capabilities research, (ii) use the subprocesses of capability bundling—stabilizing (building), enriching (developing), and pioneering (creating)—as a framework for reviewing SCM research, (iii) review 236 articles from 66 journals to develop an integrative framework of capability bundling for SCM effectiveness, (iv) discuss the findings from the review for SCM capability bundling, and (v) delineate a comprehensive research agenda that provides specific insights into how firms can come to have SCM capabilities for competitive advantages in the marketplace. In doing so, we hope to initiate a silo‐breaking, cross‐disciplinary research program on how firms bundle capabilities for competitive advantages.
Protective effects of activated vitamin D receptor on radiation‐induced intestinal injury
Authors: Lin Yuhan , Xia Penglin , Cao Fangyu , Zhang Cheng , Yang Yajie , Jiang Haitao , Lin Haishan , Liu Hu , Liu Ruling , Liu Xiaodong , Cai Jianming 
Published: December 2022

AbstractRadiation‐induced intestinal injury (RIII) is a common complication after radiation therapy in patients with pelvic, abdominal, or retroperitoneal tumours. Recently, in the model of DSS (Dextran Sulfate Sodium Salt) ‐induced intestinal inflammatory injury, it has been found in the study that transgenic mice expressing hVDR in IEC (Intestinal Epithelial Cell) manifest highly anti‐injury properties in colitis, suggesting that activated VDR in the epithelial cells of intestine may inhibit colitis by protecting the mucosal epithelial barrier. In this study, we investigated the effect of the expression and regulation of VDR on the protection of RIII, and the radiosensitivity in vitro experiments, and explored the initial mechanism of VDR in regulating radiosensitivity of IEC. As a result, we found that the expression of VDR in intestinal tissues and cells in mice can be induced by ionizing radiation. VDR agonists are able to prolong the average survival time of mice after radiation and reduce the radiation‐induced intestinal injury. For lack of vitamin D, the radiosensitivity of intestinal epithelial cells in mice increased, which can be reduced by VDR activation. Ensuing VDR activation, the radiation‐induced intestinal stem cells damage is decreased, and the regeneration and differentiation of intestinal stem cells is promoted as well. Finally, on the basis of sequencing analysis, we validated and found that VDR may target the HIF/PDK1 pathway to mitigate RIII. We concluded that agonism or upregulation of VDR expression attenuates radiation‐induced intestinal damage in mice and promotes the repair of epithelial damage in intestinal stem cells.