bluuebunny/crossref_metadata_2025_split huggingface dataset:
Cumulative Effect of pH and Redox Triggers on Highly Adaptive Transient Coacervates
Authors: Chowdhuri Sumit , Das Saurav , Kushwaha Ritvika , Das Tanushree , Das Basab Kanti , Das Debapratim 
Published: February 2023

AbstractAn intricate synergism between multiple biochemical processes and physical conditions determines the formation and function of various biological self‐assemblies. Thus, a complex set of variables dictate the far‐from‐equilibrium nature of these biological assemblies. Mimicking such systems synthetically is a challenging task. We report multi‐stimuli responsive transient coacervation of an aldehyde‐appended polymer and a short peptide. The coacervates are formed by the disulphide linkages between the peptide molecules and the imine bond between the polymer and the peptide. Imines are susceptible to pH changes and the disulphide bonds can be tuned by oxidation/reduction processes. Thus, the coacervation is operational only under the combined effect of appropriate pH and oxidative conditions. Taking advantage of these facts, the coacervates are transiently formed under a pH cycle (urea‐urease/gluconolactone) and a non‐equilibrium redox cycle (TCEP/H2O2). Importantly, the system showed high adaptability toward environmental changes. The transient existence of the coacervates can be generated without any apparent change in size and shape within the same system through the sequential application of the above‐mentioned nonequilibrium reaction cycles. Additionally, the coacervation allows for efficient encapsulation/stabilisation of proteins. Thus, the system has the potential to be used for protein/drug delivery purposes in the future.
Nanopore Deciphering Single Digital Polymers Towards High‐Density Data Storage
Authors: Hu Zheng‐Li , Liu Yu‐Hang , Xin Kai‐Li , Yu Ru‐Jia , Zhang Li‐Min , Ying Yi‐Lun 
Published: February 2023

AbstractSequence‐defined polymer is one of the most promising alternative media for high‐density data storage. It could be used to alleviate the problem of insufficient storage capacity of conventional silicon‐based devices for the explosively increasing data. To fulfil the goal of polymer data storage, suitable methods should be developed to accurately read and decode the information‐containing polymers, especially for those composed by a combination of the natural and unnatural monomers. Nanopore‐based approaches have become one of the most competitive analysis and sequencing techniques, which are expected to read both natural and synthetic polymers with single‐molecule precision and monomeric resolution. Herein, this work emphasizes the advances being made in nanopore reading and decoding of information stored in the man‐made polymers and DNA nanostructures, and discusses the challenges and opportunities towards the development and realization of high‐density data storage.
Rhodamine‐Sensitized Two‐Photon Activation of a Red Light‐Absorbing BODIPY Photocage
Authors: Weber Rebekka , Chok Kerby , Junek Stephan , Glaubitz Clemens , Heckel Alexander 
Published: February 2023

AbstractTwo‐photon (2P) activatable probes are of high value in biological and medical chemistry since near infrared (NIR) light can penetrate deeply even in blood‐perfused tissue and due to the intrinsic three‐dimensional activation properties. Designing two‐photon chromophores is challenging. However, the two‐photon absorption qualities of a photocage can be improved with an intramolecular sensitizer, which transfers the absorbed light onto the cage. We herein present the synthesis and photophysical characterization of a 2P‐sensitive uncaging dyad based on rhodamine 101 as donor fluorophore and a redshifted BODIPY as acceptor photocage. Liberation of p‐nitroaniline (PNA) upon one‐photon photolysis was confirmed by HPLC analysis. The photoreaction was found to be accompanied by a considerable change of the fluorescence properties of the chromophores. The possibility of a fluorescent read‐out enabled the detection of two‐photon induced uncaging by confocal fluorescence microscopy.
Biokatalytische Ganzzellproduktion des Sesquiterpens Presilphiperfolan‐8β‐ol in stoffwechseloptimierten Escherichia coli
Authors: Wildhagen Maik , Pudenz Tabea , Nguyen Trang , Kirschning Andreas , Beutel Sascha 
Published: February 2023

AbstractDie fermentative Ganzzellproduktion in Escherichia coli ermöglicht die Produktion komplexer Naturstoffe, wie Terpene, aus einfachen, günstigen und nachhaltigen C‐Quellen. Ziel der Arbeit war es, einen solchen Prozess zur Synthese von Presilphiperfolan‐8β‐ol (PSP) zu entwickeln. Der Biosyntheseweg dieses tricyclischen Sesquiterpenalkohols konnte erfolgreich in E. coli eingebracht werden. Der resultierende Multiplasmid‐Stamm ist in der Lage, in vivo über den Mevalonat‐Weg Farnesylpyrophosphat zu bilden, welches dann durch die Sesquiterpencyclase BcBOT2 zu PSP umgesetzt wird. Das überwiegend in das Kulturmedium sekretiert Produkt konnte via GC‐MS identifiziert und via GC‐FID quantifiziert werden. Der entwickelte Stamm produzierte in vivo bei 20 °C in 48 h 10 mg L−1 PSP direkt aus der C‐Quelle Glycerin.
ACHEMA 2022: Membranes and Membrane Processes
Authors: Götze Katja , Haseneder Roland , Herdegen Volker 
Published: February 2023

AbstractNumerous companies working in the field of membrane development, membrane production, and membrane separation engineering were present at ACHEMA 2022. Compared to the last ACHEMA in 2018, there were fewer attending companies and only few new topics since development has been blocked partially by the pandemic situation and the subsequent restrictions of this years. Nevertheless, some new developments have been identified concerning process design as well as ceramic and polymeric systems.
Impaired social functioning in adolescent and young adult sarcoma survivors: Prevalence and risk factors
Authors: Drabbe Cas , Coenraadts Elena S. , van Houdt Winan J. , van de Sande Michiel A. J. , Bonenkamp Johannes J. , de Haan Jacco J. , Nin Johanna W. M. , Verhoef Cornelis , van der Graaf Winette T. A. , Husson Olga 
Published: February 2023

AbstractBackgroundSarcomas account for almost 11% of all cancers in adolescents and young adults (AYAs; 18–39 years). AYAs are increasingly recognized as a distinct oncological age group with its own psychosocial challenges and biological characteristics. Social functioning has been shown to be one of the most severely affected domains of health‐related quality of life in AYA cancer survivors. This study aims to identify AYA sarcoma survivors with impaired social functioning (ISF) and determine clinical and psychosocial factors associated with ISF.MethodsAYAs from the population‐based cross‐sectional sarcoma survivorship study (SURVSARC) were included (n = 176). ISF was determined according to the European Organization for Research and Treatment of Cancer Quality of Life Questionnaire Core 30 social functioning scale, and age‐ and sex‐matched norm data were used as reference.ResultsThe median time since diagnosis was 6.2 years (range, 1.8–11.2). More than one‐quarter (28%) of AYA sarcoma survivors experienced ISF. Older age, higher tumor stage, comorbidities, lower experienced social support, uncertainty in relationships, feeling less attractive, sexual inactivity, unemployment, and financial difficulties were associated with ISF. In a multivariable analysis, unemployment (OR, 3.719; 95% CI, 1.261–10.967) and having to make lifestyle changes because of financial problems caused by one's physical condition or medical treatment (OR, 3.394; 95% CI, 1.118–10.300) were associated with ISF; better experienced social support was associated with non‐ISF (OR, 0.739; 95% CI, 0.570–0.957).ConclusionMore than one‐quarter of AYA sarcoma survivors experience ISF long after diagnosis. These results emphasize the importance of follow‐up care that is not only disease‐oriented but also focuses on the psychological and social domains.Plain Language Summary

Sarcomas account for almost 11% of all cancers in adolescents and young adults (AYAs; 18–39 years). The AYA group is increasingly recognized as a distinct oncological age group with its own psychosocial challenges and biological characteristics.

Social functioning has been shown to be severely affected in AYA cancer survivors.

A population‐based questionnaire study to identify AYA sarcoma survivors with impaired social functioning (ISF) and determine factors associated with ISF was conducted. More than one‐quarter of AYA sarcoma survivors experience ISF long after diagnosis. These results emphasize the importance of follow‐up care that is not only disease‐orientated but also focuses on the psychological and social domains.
Malignant rhabdoid tumour of liver: Conundrum on ascitic fluid
Authors: Sharma Rashim , Thirunavukkarasu Balamurugan , Nalwa Aasma , Pathak Manish , Garg Pawan Kumar 
Published: February 2023

AbstractAscitic fluid analysis is an important tool for diagnosis and staging. Cytological analysis is routinely done as a part of workup for ascites. This is challenging in paediatric malignancies where multiple differentials need to be considered at times with limited cellularity. We present a case of malignant rhabdoid tumour of liver in a young child presenting with abdominal lump and ascites. The diagnosis was offered on ascitic fluid cytology based on cytomorphology, supporting immunohistochemistry and later confirmed on biopsy. This report briefly discusses its differentials and approach to diagnosis.
Butyrate prevents the migration and invasion, and aerobic glycolysis in gastric cancer via inhibiting Wnt/β‐catenin/c‐Myc signaling
Authors: Liang Yizhi , Rao Zilan , Du Dongwei , Wang Yiwen , Fang Taiyong 
Published: February 2023

AbstractGastric cancer (GC) remains a common cause of cancer death worldwide. Evidence has found that butyrate exhibited antitumor effects on GC cells. However, the mechanism by which butyrate regulate GC cell proliferation, migration, invasion, and aerobic glycolysis remains largely unknown. The proliferation, migration, and invasion of GC cells were tested by EdU staining, transwell assays. Additionally, protein expressions were determined by western blot assay. Next, glucose uptake, lactate production, and cellular ATP levels in GC cells were detected. Furthermore, the antitumor effects of butyrate in tumor‐bearing nude mice were evaluated. We found, butyrate significantly prevented GC cell proliferation, migration, and invasion (p < .01). Additionally, butyrate markedly inhibited GC cell aerobic glycolysis, as shown by the reduced expressions of GLUT1, HK2, and LDHA (p < .01). Moreover, butyrate notably decreased nuclear β‐catenin and c‐Myc levels in GC cells (p < .01). Remarkably, through activating Wnt/β‐catenin signaling with LiCl, the inhibitory effects of butyrate on the growth and aerobic glycolysis of GC cells were diminished (p < .01). Moreover, butyrate notably suppressed tumor volume and weight in GC cell xenograft nude mice in vivo (p < .01). Meanwhile, butyrate obviously reduced nuclear β‐catenin, c‐Myc, GLUT1, HK2 and LDHA levels in tumor tissues in GC cell xenograft mice (p < .01). Collectively, butyrate could suppress the growth and aerobic glycolysis of GC cells in vitro and in vivo via downregulating wnt/β‐catenin/c‐Myc signaling. These findings are likely to prove useful in better understanding the role of butyrate in GC.
Phosphonylated tyrosine and lysine residues as biomarkers of local exposure of human hair to the organophosphorus nerve agents sarin and VX
Authors: John Harald , Lindl Tamara , Reuter Henrik , Schmeißer Wolfgang , Schrader Michael , Thiermann Horst 
Published: February 2023

AbstractWe herein present for the first time a micro liquid chromatography‐electrospray ionization high‐resolution tandem‐mass spectrometry (μLC‐ESI MS/HR MS) procedure to detect phosphonylated tyrosine (Tyr) and lysine (Lys) residues obtained from human hair exposed to organophosphorus nerve agents (OPNA). In general, toxic OPNA react with endogenous blood proteins causing the formation of adducts representing well‐known targets for biomedical analysis to prove exposure. In contrast, no protein‐derived biomarker has been introduced so far to document local exposure of hair. Accordingly, we developed and characterized a μLC‐ESI MS/HR MS method for the analysis of scalp hair exposed to OPNA in vitro. Type I and Type II keratin from hair was dissolved during lysis, precipitated and subjected to pronase‐catalyzed hydrolysis yielding single adducted Lys and in a much higher amount Tyr residues. Exposure to sarin caused the adduction of an isopropyl methylphosphonic acid moiety and exposure to VX yielded adducts of ethyl methylphosphonic acid, well suited as biomarkers of exposure. These were of appropriate stability in the autosampler for 24 h. The biomarker yield obtained from hair of six individuals as well as from hair of six different parts of the body of one individual (armpit, beard, leg, arm, scalp, and pubic) differed reasonably indicating the variable individual protein composition and structure of hair. Exposed hair stored at ambient temperature for 9 weeks with contact to air and daylight showed stability of all adducts and therefore their suitability for verification of exposure.
Experiences of loss of control eating in women after bariatric surgery: A qualitative study
Authors: Yu Yang , Yeh Kuan‐Lin , Kalarchian Melissa A. , Groth Susan 
Published: February 2023

AbstractObjectiveLoss of control (LOC) eating following bariatric surgery remains insufficiently understood, reflected in a lack of clear conceptualization, valid measurements, and effective treatments. This study explored patients' perspectives on LOC eating post‐bariatric surgery, focusing on the relevance of the Diagnostic and Statistical Manual of Mental Disorders‐5 (DSM‐5) indicators of impaired control, and patients' experiences before (precipitating factors), during (eating patterns, perpetuating factors), and after the episodes (strategies used to stop eating).MethodsThis was a qualitative study using descriptive phenomenology. Participants were adults who have undergone gastric bypass or sleeve gastrectomy and self‐reported recent LOC eating. Data were collected by semi‐structured interviews and analyzed using a combined inductive and deductive approach.ResultsParticipants were all women (N = 15; age: 34.5 ± 9.2 years). Results suggested that (1) except for feeling disgusted, guilty, or depressed after eating, other DSM‐5 indicators did not seem to be highly relevant to this bariatric cohort; (2) LOC eating could occur across contexts, with food access, boredom, and food craving being consistent triggers; (3) the food amount consumed during a LOC episode was not necessarily considered excessive; (4) “mindlessness” and “satisfying hedonic, physiological, and mental needs” were the main perpetuating factors of LOC eating; and (5) self‐talk and distracting attention were the most adopted strategies to stop eating.DiscussionStudy findings have implications concerning the conceptualization, measurement, and intervention of LOC eating among bariatric patients. For example, results suggested the need for more data to determine the appropriate indicators of LOC eating and the inclusion of boredom as an intervention target in bariatric patients.Public SignificanceLoss of control (LOC) eating is characterized by a sense of being unable to stop while eating. LOC eating is prevalent in patients who have undergone weight loss surgery and is associated with worse surgical outcomes. This qualitative study that explored post‐bariatric surgery patients' experiences of LOC eating will inform efforts to better assess and intervene in this disordered eating behavior, thus ultimately optimizing patients' health following weight loss surgery.
The future of suitable habitats of an endangered Neotropical grassland bird: A path to extinction?
Authors: Meireles Ricardo C. , Lopes Leonardo E. , Brito Gustavo R. , Solar Ricardo 
Published: February 2023

AbstractGlobal changes increasingly worry researchers and policymakers and may have irreversible impacts on Earth's biodiversity. Similar to other phytophysiognomies, natural grasslands suffer from the effects of land use changes and rising temperatures, threatening animal and plant communities. Birds, being very sensitive to these changes, are widely studied and fundamental to understand the dynamics of ecosystems in relation to climate and land use changes. The Campo Miner Geositta poeciloptera is a grassland bird endemic to the Brazilian Cerrado and threatened with extinction that has been widely studied in recent years. We analyze the decrease in its extent of occurrence (EOO) and the effects of climate and land use change to understand the environmental suitability of the species in current and future scenarios. We used 5 common algorithms to produce ecological niche models. For future predictions, we use two general circulation models for two different greenhouse gas emission scenarios with different climate policies, an optimistic (ssp245) and a pessimistic (ssp585), plus two land use models focusing on increasing farmlands and reducing native grasslands. The current EOO represents ~45% of that presented by the IUCN EOO. The models generated for the present were satisfactory (TSS = 0.77 and ROC = 0.90) and showed high environmental suitability in areas where the species is currently found and low suitability where it is already extinct. All future scenarios have reduced suitable areas for the species, and the models of a greater increase in temperature and increase in farmlands and a greater decrease in grasslands were the worse. Our results reinforce the need to care about biome awareness disparity and the importance of actively preserving grassy‐shrub areas. Apparently, the state of Minas Gerais will be the only stronghold of the species in the coming years; however, the lack of protected areas that guarantee its survival needs attention.
A study on improving shielding performance of shielded cable
Authors: Tsuchida Takashi 
Published: February 2023

AbstractWith the developments in power electronics equipment, the electromagnetic environment has become severe for electronic equipment. In the smart facility field, the stable operation of electronic devices is indispensable, and noise countermeasures must be improved. To suppress intrusive noise from cables, shielded cables are used in many cases. However, electromagnetic induction cannot be suppressed when the shield is grounded at one end, and when grounding both ends of the shield, current flows through the shield creating intrusive noise in the cable. That is, even if a shielded cable is used, a sufficient effect cannot be obtained. Therefore, as a countermeasure, this study prototypes and evaluates cables that combine double shielded cables with magnetic cores. Evaluations conducted using the copper pipe method, triaxial method, and impulse test confirm that the shielded performance improved by 10–30 dB compared with the conventional shielded cable.
STAT6 controls the stability and suppressive function of regulatory T cells
Authors: Arroyo‐Olarte Rubén D. , Rivera‐Rugeles Ana , Nava‐Lira Eduardo , Sánchez‐Barrera Ángel , Ledesma‐Soto Yadira , Saavedra Rafael , Armas‐López Leonel , Terrazas Luis I , Ávila‐Moreno Federico , Leon‐Cabrera Sonia 
Published: February 2023

AbstractSignal transducer and activator of transcription 6 (STAT6) promotes tumorigenesis by decreasing the Forkhead box P3+ (Foxp3+) cell frequency allowing for the infiltration of inflammatory cells during the early stages of colitis‐associated cancer (CAC). In this study, we dissected the role of STAT6 in the generation of inducible in vitro regulatory T cells (iTregs) and peripheral in vivo Tregs (pTregs) under inflammatory conditions. In in vitro assays, when STAT6 was lacking, iTregs preserved a stable phenotype and expressed high levels of Foxp3 and CD25 during long expansion periods, even in the presence of IL‐6. This effect was associated with increased in vitro suppressive ability, over‐expression of programmed death‐1 (PD‐1), CTLA‐4, and Foxp3, and decreased IFN‐γ expression. Furthermore, iTregs developed during STAT6 deficiency showed a higher demethylation status for the FOXP3 Treg‐specific demethylated region (TSDR), coupled with lower DNA methyltransferase 1 (DNMT1) mRNA expression, suggesting that STAT6 may lead to Foxp3 silencing. Using a mouse model of CAC, the STAT6‐/‐ pTregs expressed a more activated phenotype at the intestine, had higher suppressive capacity, and expressed more significant levels of PD‐1 and latency‐associated peptide of TGF‐β (LAP) associated with their ability to attenuate tumor development. These data suggest that STAT6 signaling impairs the induction, stability, and suppressive capacity of Tregs developed in vitro or in vivo during gut inflammation.
Coal gangue detection and recognition method based on multiscale fusion lightweight network SMS‐YOLOv3
Authors: Li Deyong , Ren Huaiwei , Wang Guofa , Wang Shuang , Wang Wenshan , Du Ming 
Published: February 2023

AbstractAiming at the problems of large memory footprint, low detection speed, and low detection accuracy for small and overlapping targets existing in the current coal gangue target detection algorithm, a real‐time detection method for coal gangue based on a multiscale fusion lightweight network (SMS‐YOLOv3) is proposed. Taking MobileNetv3 as a feature extraction network, in which all SE modules are replaced with SKNet, thus improving the ability of image feature extraction and making more effective use of parameters. A shallow detection scale is added to form a detection structure with the fusion of four scales to improve the detection accuracy of small targets. The spatial pyramid pooling is added after the backbone network to convert different feature maps into fixed feature maps, to improve the detection accuracy of the algorithm. CIoU bounding box regression loss and the K‐means++ clustering anchorbox are used to improve the detection accuracy of targets. Experimental equipment was built, and coal gangue datasets of small size, large size, dim light, mutual concealment, and a large number of coal gangue under multiple conditions were constructed. Experiment results demonstrate the effective and fast detection of the proposed algorithm for small targets and overlapping targets of coal gangue accurately, with mAP reaching 98.97%. The algorithm has an mAP improvement of 0.37% and an fps increase of 119.04% compared with the original YOLOv3, with memory only 1/24 of the original.
X‐Ray and NMR Structural Data of Ethynylbenziodoxolones (EBXs) Reagents and Their Analogues
Authors: Le Du Eliott , Ramirez Nieves P. , Nicolai Stefano , Scopelliti Rosario , Fadaei‐Tirani Farzaneh , Wodrich Matthew D. , Hari Durga Prasad , Waser Jerome 
Published: February 2023

AbstractFirst synthesized in 1991, EthynylBenziodoXolones (EBXs) – cyclic hypervalent iodine reagents derived from 2‐iodobenzoic acid – are now among the most versatile electrophilic alkynylation reagents. Due to their cyclic structure, these reagents exhibit enhanced stability compared to previously used alkynyl iodonium salts. Over the last decade, both the iodoheterocycle and the arene ring have been extensively modified to fine‐tune the reactivity of the reagents, resulting in new analogues such as Ethynylbenziodoxoles (EBxs) or N‐heterocyclic reagents. In this article, we have for the first time compiled the structural data available for EBXs and their analogues, focusing especially on X‐Ray and NMR data. For selected compounds, molecular electrostatic potentials (MEP) have also been calculated. When considering the tight relationship between structure and reactivity in hypervalent iodine reagents, the collected data is expected to be highly useful for further developments in the field.