bluuebunny/crossref_metadata_2025_split huggingface dataset:
Mechanism of cystogenesis by Cd79a-driven, conditional mTOR activation in developing mouse nephrons
Authors: Tran Nguyen Truc Linh , Matsuda Satoshi , Takenouchi Akiko , Tran Thuy Huong Quynh , Kotani Yui , Miyazaki Tatsuhiko , Kanda Hiroaki , Yoshizawa Katsuhiko , Tsukaguchi Hiroyasu 
Published: January 2023

AbstractPolycystic kidney disease (PKD) is a common genetic disorder arising from developmental and postnatal processes. Defects in primary cilia and their signaling (eg, mTOR) underlie the pathogenesis. However, how mTOR regulates tubular integrity remains unclear. The paucity of faithful models has limited our understanding of pathogenesis and, therefore, the refinement of therapeutic targets. To understand the role of mTOR in early cystogenesis, we studied an in-house mouse model, Cd79a-Cre;Tsc1ff. (Cd79a-Tsc1 KO hereafter), recapitulating human autosomal-dominant PKD histology. Cre-mediated Tsc1 depletion driven by the promoter for Cd79a, a known B-cell receptor, activated mTORC1 exclusively along the distal nephron from embryonic day 16 onward. Cysts appeared in the distal nephron at 1 weeks of age and mice developed definite PKD by 4 weeks. Cd79a-Tsc1 KO tubule cells proliferated at a rate comparable to controls after birth but continued to divide even after postnatal day 14 when tubulogenesis is normally completed. Apoptosis occurred only after 9 weeks. During postnatal days 7–11, pre-cystic Cd79a-Tsc1 KO tubule cells showed cilia elongation, aberrant cell intercalation, and mitotic division, suggesting that defective cell planar polarity (PCP) may underlie cystogenesis. mTORC1 was activated in a portion of cyst-lining cells and occasionally even when Tsc1 was not depleted, implying a non-autonomous mechanism. Our results indicate that mTORC1 overactivation in developing distal tubules impairs their postnatal narrowing by disrupting morphogenesis, which orients an actively proliferating cell toward the elongating axis. The interplay between mTOR and cilium signaling, which coordinate cell proliferation with PCP, may be essential for cystogenesis.
Detailed morphological structure and phylogenetic relationships of Degeeriella punctifer (Phthiraptera: Philopteridae), a parasite of the bearded vulture Gypaetus barbatus (Accipitriformes: Accipitridae)
Authors: Pérez Jesús M. , Sáez-Ventura Ángeles , Liébanas Gracia , Rossi Luca , Fernández Mercedes , Fraija-Fernández Natalia 
Published: January 2023

AbstractHabitat loss is one of the main threats to species survival and, in the case of parasites, it is their hosts that provide their habitat. Therefore, extinction even at local scale of host taxa also implies the extinction of their parasites in a process known as co-extinction. This is the case of the bearded vulture (Gypaetus barbatus), which almost became extinct at the beginning of the twentieth century. After several attempts, this species was successfully reintroduced into the Alps at the end of the twentieth century. We collected 25 lice specimens from an electrocuted bearded vulture from Susa (Italian Alps) that were morphologically identified asDegeeriella punctifer. Six individuals were studied by scanning electron microscopy, with particular emphasis on their cephalic sensorial structures, while four further specimens were characterized at molecular level by amplifying partial regions of the12SrRNA,COX1and elongation factor 1 alpha (EF-1) genes. From a morphological perspective, the number, type and arrangement of the sensillae on the two distal antennal segments is quite similar to that of other species of the family Philopteridae (Phthiraptera: Ischnocera). The mandibles and tarsal claws allow lice to cling firmly to their host’s feathers. Phylogenetic analyses help unravel the paraphyletic nature of the genusDegeeriellaand demonstrate the clear differentiation between lice parasitizing Accipitriformes and Falconiformes, as well as the close relationship betweenD. punctifer, D. fulva, D. nisusandCapraiellasp. that, along with other genera, parasitize rollers (Aves: Coraciiformes).
Evolution of field induced magnetic phase attributed to higher order magnetic moments in TbVO4
Authors: Ranaut Dheeraj , Mukherjee K. 
Published: January 2023

AbstractStudy of quantum magnetism in rare earth orthovanadates (RVO4, R = rare earth) is a topic which is currently being investigated by the condensed matter physicists. In this work, through both experimental and theoretical tools, we report the presence of field induced magnetic phase, attributed to fifth order susceptibility, in TbVO4, at low temperatures. The structural transition reported around 31 K, results in the formation of pseudospin—$${\raise0.7ex\hbox{$1$} \!\mathord{\left/ {\vphantom {1 2}}\right.\kern-0pt} \!\lower0.7ex\hbox{$2$}}$$

1

/

2

doublet ground state separated by an energy δ. Temperature dependent heat capacity indicates toward an increment in δ, on application of magnetic fields. Above 10 kOe, the Zeeman energy associated with magnetic anisotropy strengthens, resulting in an enhanced splitting of the pseudo-doublet ground state. This increased splitting stabilizes the magnetic phase associated with higher order moments. These observations are further supported by our theoretical model to evaluate δ, as a function of applied field. Our study provides a platform to study the possible presence of higher order moments in other Jahn–Teller systems.
An Interactive and Neurodiversity-Affirming Approach to Communication Supports for Autistic Students Through Videogaming
Authors: Santhanam Siva priya 
Published: January 2023

Purpose:
Videogaming offers an interactive, enjoyable, and socially valid context for interaction between autistic and non-autistic students in schools. In this tutorial, I describe an approach that supports communication and peer interaction through group-based videogaming. This approach creates an opportunity for autistic and non-autistic students to engage in a meaningful and enjoyable activity and to promote acceptance and appreciation of communication differences.

Method:
This tutorial includes development, planning, essential components, goal writing, and a list of resources and directions to implement the videogaming-based group intervention in middle and high school settings. This tutorial is grounded in a supports-based, anti-ableist, neurodiversity framework that embraces a difference-based view of autistic communication. Cost-effectiveness and ease of implementation make this intervention feasible for school settings.

Conclusions:
Ableist practices are pervasive in academic and clinical settings. There is an urgency within the field of speech-language pathology to address misconceptions regarding autism and autistic individuals, increase the understanding and appreciation of diversity in autistic communication, and eliminate practices that may promote autistic masking. These can be achieved by developing supports that value the preferences and experiences of autistic individuals and honor their strengths. Videogaming is one context that supports social communication. It does not hinge upon normative communication as a benchmark. Increased contact and exposure to autistic peers through fun and engaging game-based interactions help reduce stigma and prejudice toward autistic students.
Teacher Questioning to Promote Extended Language Use for Preschool Emergent Bilinguals
Authors: Buckley Laura , Mancilla-Martinez Jeannette , Ozdemir Merve 
Published: January 2023

Purpose:
Emergent bilinguals (EBs) from Spanish-speaking households are a sizable and quickly growing segment of the preschool population in the United States. However, there is limited research on the provision of opportunities for EBs to engage in language-rich classroom discussion, particularly in English-dominant contexts where most EBs attend preschool. This study focused on teacher and Spanish–English EBs' language interactions in an English-dominant preschool program to better understand whether and, if so, how teachers' use of questioning strategies provided extended oral language use opportunities for Spanish-speaking EBs in their classrooms.

Method:

We adopted a sequential–explanatory mixed-methods design to examine audio recordings from whole-group instruction across seven preschool classrooms and investigate how EBs responded to teachers' conversationally responsive questioning strategies, with a specific focus on how they used Spanish as they composed extended responses. Researchers coded 31 audio recordings from 12 EB students to identify teachers' (
n
= 7) use of questioning strategies (closed-response, open-response, and single-word-response), as well as students' responses to questions (one-word-response or extended response) and Spanish use.

Results:
Teachers' use of closed-response and single-word-response questions emerged as most important in supporting Spanish–English EBs' extended language use during whole-group instruction. Furthermore, the majority of student responses that included Spanish utterances were extended responses, underscoring the value of Spanish use for students to develop extended responses.

Conclusion:
Findings suggest that equitable opportunities to enter into classroom dialogue for EBs might require more explicitly scaffolded questioning strategies and might necessitate the purposeful and intentional use of Spanish.
Reducing carbon emissions and energy costs in multi‐building facilities: A Co‐optimisation approach
Authors: Abbas Akintonde , Chowdhury Badrul 
Published: January 2023

AbstractCommercial buildings remain one of the most significant consumers of energy. As such, any sustainable pathway to a zero‐emissions future will need to pay close attention to emissions reduction in commercial buildings. An interesting category of commercial buildings is the multi‐building commercial facility with different buildings collocated within a defined geographical area, serving different purposes and containing varying equipment types. While most existing facility management approaches focus on minimising energy costs and emissions at the level of each building, this work considers a different perspective where energy and emissions are co‐optimised across all buildings within the facility. Illustrative case studies based on a multi‐building facility consisting of individual buildings adapted from the United States Department of Energy's (DOE) Commercial Reference Buildings database are considered. Different diurnal and seasonal variations in building usage are also considered. Simulations are run using a Python‐based commercial building simulation toolbox. Results indicate that the co‐optimisation approach can indeed provide superlinear emissions reductions and energy cost savings while satisfying predefined comfort limits compared to when each building is separately optimised.
A 2022τ-Herculid meteor cluster from an airborne experiment: automated detection, characterization, and consequences for meteoroids
Authors: Vaubaillon J. , Loir C. , Ciocan C. , Kandeepan M. , Millet M. , Cassagne A. , Lacassagne L. , Da Fonseca P. , Zander F. , Buttsworth D. , Loehle S. , Toth J. , Gray S. , Moingeon A. , Rambaux N. 
Published: January 2023

Context. The existence of meteor clusters has long since been a subject of speculation and so far only seven events have been reported, among which two involve less than five meteors, and three were seen during the Leonid storms.Aims. The 1995 outburst of Comet 73P/Schwassmann-Wachmann was predicted to result in a meteor shower in May 2022. We detected the shower, proved this to be the result of this outburst, and detected another meteor cluster during the same observation mission.Methods. Theτ-Herculids meteor shower outburst on 31 May 2022 was continuously monitored for 4 h during an airborne campaign. The video data were analyzed using a recently developed computer-vision processing chain for meteor real-time detection.Results. We report and characterize the detection of a meteor cluster involving 38 fragments, detected at 06:48 UT for a total duration of 11.3 s. The derived cumulative size frequency distribution index is relatively shallow:s= 3.1. Our open-source computer-vision processing chain (named FMDT) detects 100% of the meteors that a human eye is able to detect in the video. Classical automated motion detection assuming a static camera was not suitable for the stabilized camera setup because of residual motion.Conclusions. From all reported meteor clusters, we crudely estimate their occurrence to be less than one per million observed meteors. Low heliocentric distance enhances the probability of such meteoroid self-disruption in the interplanetary space.
Offset between X-ray and optical centers in clusters of galaxies: Connecting eROSITA data with simulations
Authors: Seppi R. , Comparat J. , Nandra K. , Dolag K. , Biffi V. , Bulbul E. , Liu A. , Ghirardini V. , Ider-Chitham J. 
Published: January 2023

Context.The characterization of the dynamical state of galaxy clusters is key to studying their evolution, evaluating their selection, and using them as a cosmological probe. In this context, the offsets between different definitions of the center have been used to estimate the cluster disturbance.Aims.Our goal is to study the distribution of the offset between the X-ray and optical centers in clusters of galaxies. We study the offset for clusters detected by the extended ROentgen Survey with an Imaging Telescope Array (eROSITA) on board the Spectrum-Roentgen-Gamma (SRG) observatory. We aim to connect observations to predictions by hydrodynamical simulations andN-body models. We assess the astrophysical effects affecting the displacements.Methods.We measured the offset for clusters observed in the eROSITA Final Equatorial-Depth Survey (eFEDS) and the first eROSITA all-sky survey (eRASS1). We focus on a subsample of 87 massive eFEDS clusters at low redshift, withM500c> 1×1014 M⊙and 0.15 <z< 0.4. We compared the displacements in such sample to those predicted by the TNG and the Magneticum simulations. We additionally link the observations to the offset parameterXoffmeasured for dark matter halos inN-body simulations, using the hydrodynamical simulations as a bridge.Results.We find that, on average, the eFEDS clusters show a smaller offset compared to eRASS1 because the latter contains a larger fraction of massive and disturbed structures. We measured an average offset of ΔX−O = 76.3−27.1+30.1kpc, when focusing on the subsample of 87 eFEDS clusters. This is in agreement with the predictions from TNG and Magneticum, and the distribution ofXofffrom dark matter only (DMO) simulations. However, the tails of the distributions are different. Using ΔX − Oto classify relaxed and disturbed clusters, we measured a relaxed fraction of 31% in the eFEDS subsample. Finally, we found a correlation between the offset measured on hydrodynamical simulations andXoffmeasured on their parent dark-matter-only run and we calibrated the relation between them.Conclusions.We conclude that there is good agreement between the offsets measured in eROSITA data and the predictions from simulations. Baryonic effects cause a decrement (increment) in the low (high) offset regime compared to theXoffdistribution from dark matter-only simulations. The offset–Xoffrelation provides an accurate prediction of the trueXoffdistribution in Magneticum and TNG. It allows for the offsets to be introduced in a cosmological context with a new method in order to marginalize over selection effects related to the cluster dynamical state.
MicroRNA-32 Suppression: its Effects on Prostate Cancer
Cells’ Capability to Proliferate and Migrate
Authors: Al-Marzook Farah A. , Hassan Duha Maithem , Alghazal Maha Waleed , Kadheem Rana Abd Alameer , Jalil Abduladheem Turki , Saleh Marwan Mahmood 
Published: January 2023

Abstract
Introduction This paper sought to scrutinize the role of microRNA-32
(miR-32) on the growth and migration as well as on the expression of metastatic
genes in PC3 cells of prostate cancer in vitro.
Methods Subsequent transfection of cells with miR-32 mimics, miR-32
inhibitor, negative control (NC), cell proliferation using MTT, and apoptosis by
ELISA were performed. Furthermore, qRT-PCR was directed to measure the
expression levels of matrix metalloproteinase 2 (MMP2) and vascular endothelial
growth factors (VEGF) as metastatic and angiogenesis genes in the progression of
PC3.
Results miR-32 was overexpressed in PC3 cells compared to normal cells
(P<0.001). Down-regulation of miR-32 obstructs in vitro
proliferation and migration while intensifying the apoptosis rate in PC3 cells.
Also, we found that miR-32 negatively modulates the expression of VEGF and MMP2
in PC3 cells.
Conclusion These results indicate that the suppression of miR-32 might
offer an auxiliary treatment procedure for addressing the invasion, progression,
and metastasis in PCa patients by improving cell apoptosis.
Acteoside from Conandron ramondioides Reduces Microcystin-LR Cytotoxicity by Inhibiting Intracellular Uptake Mediated by OATP1B3
Authors: Takumi Shota , Hashimoto Kairi , Tomioka Masaru , Sato Mina , He Weijie , Komatsu Yumiko , Aoki Shunji , Ikeda Ryuji , Shiozaki Kazuhiro , Furukawa Tatsuhiko , Komatsu Masaharu 
Published: January 2023

AbstractThe hepatotoxin microcystin-LR is a strong inhibitor of serine/threonine protein phosphatase (PP) 1 and PP2A. The onset of its cytotoxicity depends on its selective uptake via the
hepatocyte uptake transporters, organic anion transporting polypeptide (OATP) 1B1 and OATP1B3. Understanding and preventing the cytotoxicity of microcystin-LR is crucial to maintain human
health. This chemoprevention study demonstrates that the herbal plant extract of iwajisha (20 µg/mL) reduced microcystin-LR cytotoxicity in OATP1B3-expressing cells by approximately six
times. In addition, 20 µM acteoside, which is one of the major compounds in iwajisha, reduced microcystin-LR cytotoxicity by approximately 7.4 times. Acteoside could also reduce the
cytotoxicity of other compounds, such as okadaic acid and nodularin, which are both substrates of OATP1B3 and inhibitors of PP1/PP2A. To investigate the mechanism by which the cytotoxicity
of microcystin-LR is attenuated by acteosides, microcystin-LR and microcystin-LR-binding proteins in cells were examined after microcystin-LR and acteosides were co-exposed. Thus, acteoside
noncompetitively inhibited microcystin-LR uptake by OATP1B3-expressing cells. Furthermore, acteoside inhibited the intracellular interaction of microcystin-LR with its binding protein(s),
including the 22 kDa protein. Furthermore, using immunoblot analysis, acteoside induced the phosphorylation of extracellular signal-regulated kinase (ERK), which is one of the survival
signaling molecules. These results suggest that acteoside reduces microcystin-LR cytotoxicity through several mechanisms, including the inhibition of microcystin-LR uptake via OATP1B3, and
decreased interaction between microcystin-LR and its binding protein(s), and that ERK signaling activation contributes to the attenuation effect of acteoside against microcystin-LR
cytotoxicity.
Costs of a structured early detection program for advanced liver fibrosis and cirrhosis: insights on the “plus” of Check-up 35+
Authors: Ortner Julia , Van Ewijk Reyn Joris , Velthuis Louis , Labenz Christian , Arslanow Anita , Wörns Marcus-Alexander , Reichert Matthias Christian , Farin-Glattacker Erik , Fichtner Urs Alexander , Stelzer Dominikus , Galle Peter Robert , Lammert Frank 
Published: January 2023

Abstract
Background The implementation of an early detection program for
liver cirrhosis in a general population has been discussed for some time. Recently, the
effectiveness of a structured screening procedure, called SEAL (Structured Early detection of
Asymptomatic Liver cirrhosis), using liver function tests (AST and ALT) and APRI to early
detect advanced fibrosis and cirrhosis in participants of the German “Check-up 35” was
investigated.
Methods This study identifies the expected diagnostic costs of
SEAL in routine care and their drivers and reports on prevailing CLD etiologies in this
check-up population. The analysis is based on theoretical unit costs, as well as on the
empirical billing and diagnostic data of SEAL participants.
Results Screening costs are mainly driven by liver biopsies,
which are performed in a final step in some patients. Depending on the assumed biopsy rates
and the diagnostic procedure, the average diagnostic costs are between EUR 5.99 and 13.74 per
Check-up 35 participant and between EUR 1,577.06 and 3,620.52 per patient diagnosed with
fibrosis/cirrhosis (F3/F4). The prevailing underlying etiology in 60% of cases is
non-alcoholic fatty liver disease.
Discussion A liver screening following the SEAL algorithm could
be performed at moderate costs. Screening costs in routine care depend on actual biopsy rates
and procedures, attendance rates at liver specialists, and the prevalence of fibrosis in the
Check-up 35 population. The test for viral hepatitis newly introduced to Check-up 35 as
once-in-a-lifetime part of Check-up 35 is no alternative to SEAL.
Computer-aided diagnosis for optical diagnosis of diminutive colorectal polyps including sessile serrated lesions: a real-time comparison with screening endoscopists
Authors: Houwen Britt B. S. L. , Hazewinkel Yark , Giotis Ioannis , Vleugels Jasper L. A. , Mostafavi Nahid S. , van Putten Paul , Fockens Paul , Dekker Evelien ,   POLAR Study Group
Published: January 2023

Abstract
Background We aimed to compare the accuracy of the optical diagnosis of diminutive colorectal polyps, including sessile serrated lesions (SSLs), between a computer-aided diagnosis (CADx) system and endoscopists during real-time colonoscopy.
Methods We developed the POLyp Artificial Recognition (POLAR) system, which was capable of performing real-time characterization of diminutive colorectal polyps. For pretraining, the Microsoft-COCO dataset with over 300 000 nonpolyp object images was used. For training, eight hospitals prospectively collected 2637 annotated images from 1339 polyps (i. e. publicly available online POLAR database). For clinical validation, POLAR was tested during colonoscopy in patients with a positive fecal immunochemical test (FIT), and compared with the performance of 20 endoscopists from eight hospitals. Endoscopists were blinded to the POLAR output. Primary outcome was the comparison of accuracy of the optical diagnosis of diminutive colorectal polyps between POLAR and endoscopists (neoplastic [adenomas and SSLs] versus non-neoplastic [hyperplastic polyps]). Histopathology served as the reference standard.
Results During clinical validation, 423 diminutive polyps detected in 194 FIT-positive individuals were included for analysis (300 adenomas, 41 SSLs, 82 hyperplastic polyps). POLAR distinguished neoplastic from non-neoplastic lesions with 79 % accuracy, 89 % sensitivity, and 38 % specificity. The endoscopists achieved 83 % accuracy, 92 % sensitivity, and 44 % specificity. The optical diagnosis accuracy between POLAR and endoscopists was not significantly different (P = 0.10). The proportion of polyps in which POLAR was able to provide an optical diagnosis was 98 % (i. e. success rate).
Conclusions We developed a CADx system that differentiated neoplastic from non-neoplastic diminutive polyps during endoscopy, with an accuracy comparable to that of screening endoscopists and near-perfect success rate.