bluuebunny/crossref_metadata_2025_split huggingface dataset:
Determination of the Plasma Levels of Growth Arrest Specific 6 in Colon Cancer Patients
Authors: TEZCAN Songul , İZZETTİN Fikret , BİNGÖL ÖZAKPINAR Özlem , ATTAALLAH Vafi , YUMUK Perran , URAS Fikriye 
Published: May 2023

Objective: Growth arrest-specific 6 (GAS 6) has a role in cell proliferation and was detected in different cancer types. The aim of this study was to evaluate the plasma GAS 6 levels in colon cancer patients.
Methods: This was a prospective study and conducted in a research and training hospital in Turkey. Thirty-three healthy volunteers were enrolled in the control group, thirty-three colon cancer patients who were diagnosed with colon cancer for the first time. Sociodemographic characteristics of participants were recorded. Blood samples of the control group were taken once a time. Patients’ blood samples were taken before and one month after surgery.
Results: There were no statistically significant differences between the sociodemographic characteristics of the two groups. The mean plasma GAS 6 levels in control were significantly higher than that of colon cancer patients (p
Examination of Factors Affecting the Tendency of Intensive Care Nurses Towards Medical Errors: A Multicenter Study in Turkey
Authors: TEPEHAN ERASLAN Selma , BOZKURT Gülçin 
Published: May 2023

Objective: This study aimed to examine the factors affecting the tendency of intensive care nurses towards medical errors.
Methods: The data of this descriptive and cross-sectional were collected using a Personal Information Form and the Medical Error Trend Scale in Nursing. The population of the study consisted of a total of 647 intensive care unit nurses who worked at two research and training hospitals located in the province of Istanbul and one university hospital located in the province of Edirne. The inclusion criterion was being a registered nurse working in intensive care units for at least two months, and it was aimed to reach the entire population. The sample included 349 nurses (participation rate: 53.12%) who completed the questionnaire.
Results: While76.5% of the participants were female, their mean age was 28.96±5.70 years, and 73.4% had graduated from universities. The order of significance of the four independent variables, which were determined to have significant effects on the Medical Error Trend Scale in Nursing scores of the participants, was satisfaction with working in the intensive care unit, the number of patients per nurse, having an intensive care nurse certificate, and weekly working hours.
Conclusion: The tendencies of the nurses who worked for 40 hours per week, those who were satisfied with working in the intensive care unit, those who provided care for 1 or 2 patients per day, and those who had an intensive care nurse certificate towards medical errors were lower in comparison to the others.
Construction of a fecal immune-related protein-based biomarker panel for colorectal cancer diagnosis: a multicenter study
Authors: Zhang Hao , Zuo Lugen , Li Jing , Geng Zhijun , Ge Sitang , Song Xue , Wang Yueyue , Zhang Xiaofeng , Wang Lian , Zhao Tianhao , Deng Min , Chai Damin , Wang Qiusheng , Yang Zi , Liu Quanli , Qiu Quanwei , He Xuxu , Yang Yiqun , Ge Yuanyuan , Wu Rong , Zheng Lin , Li Jianjun , Chen Runkai , Sun Jialiang , Hu Jianguo 
Published: May 2023

PurposeTo explore fecal immune-related proteins that can be used for colorectal cancer (CRC) diagnosis.Patients and methodsThree independent cohorts were used in present study. In the discovery cohort, which included 14 CRC patients and 6 healthy controls (HCs), label-free proteomics was applied to identify immune-related proteins in stool that could be used for CRC diagnosis. Exploring potential links between gut microbes and immune-related proteins by 16S rRNA sequencing. The abundance of fecal immune-associated proteins was verified by ELISA in two independent validation cohorts and a biomarker panel was constructed that could be used for CRC diagnosis. The validation cohort I included 192 CRC patients and 151 HCs from 6 different hospitals. The validation cohort II included 141 CRC patients, 82 colorectal adenoma (CRA) patients, and 87 HCs from another hospital. Finally, the expression of biomarkers in cancer tissues was verified by immunohistochemistry (IHC).ResultsIn the discovery study, 436 plausible fecal proteins were identified. And among 67 differential fecal proteins (|log2 fold change| &gt; 1, P&lt; 0.01) that could be used for CRC diagnosis, 16 immune-related proteins with diagnostic value were identified. The 16S rRNA sequencing results showed a positive correlation between immune-related proteins and the abundance of oncogenic bacteria. In the validation cohort I, a biomarker panel consisting of five fecal immune-related proteins (CAT, LTF, MMP9, RBP4, and SERPINA3) was constructed based on the least absolute shrinkage and selection operator (LASSO) and multivariate logistic regression. The biomarker panel was found to be superior to hemoglobin in the diagnosis of CRC in both validation cohort I and validation cohort II. The IHC result showed that protein expression levels of these five immune-related proteins were significantly higher in CRC tissue than in normal colorectal tissue.ConclusionA novel biomarker panel consisting of fecal immune-related proteins can be used for the diagnosis of CRC.
Failure to decrease HbA1c levels following TB treatment is associated with elevated Th1/Th17 CD4+ responses
Authors: Krause Robert , Warren Christian M. , Simmons Joshua D. , Rebeiro Peter F. , Maruri Fernanda , Karim Farina , Sterling Timothy R. , Koethe John R. , Leslie Al , van der Heijden Yuri F. 
Published: May 2023

IntroductionThe rising global burden of metabolic disease impacts the control of endemic tuberculosis (TB) in many regions, as persons with diabetes mellitus (DM) are up to three times more likely to develop active TB than those without DM. Active TB can also promote glucose intolerance during both acute infection and over a longer term, potentially driven by aspects of the immune response. Identifying patients likely to have persistent hyperglycemia following TB treatment would enable closer monitoring and care, and an improved understanding of underlying immunometabolic dysregulation.MethodsWe measured the relationship of plasma cytokine levels, T cell phenotypes and functional responses with the change in hemoglobin A1c (HbA1c) before and after treatment of pulmonary TB in a prospective observational cohort in Durban, South Africa. Participants were stratified based on stable/increased HbA1c (n = 16) versus decreased HbA1c (n = 46) levels from treatment initiation to 12 month follow-up.ResultsCD62 P-selectin was up- (1.5-fold) and IL-10 downregulated (0.85-fold) in plasma among individuals whose HbA1c remained stable/increased during TB treatment. This was accompanied by increased pro-inflammatory TB-specific IL-17 production (Th17). In addition, Th1 responses were upregulated in this group, including TNF-α production and CX3CR1 expression, with decreased IL-4 and IL-13 production. Finally, the TNF-α+ IFNγ+ CD8+ T cells were associated with stable/increased HbA1c. These changes were all significantly different in the stable/increased HbA1c relative to the decreased HbA1c group.DiscussionOverall, these data suggest that patients with stable/increased HbA1c had an increased pro-inflammatory state. Persistent inflammation and elevated T cell activity in individuals with unresolved dysglycemia following TB treatment may indicate failure to fully resolve infection or may promote persistent dysglycemia in these individuals, and further studies are needed to explore potential mechanisms.
A fibroblast-associated signature predicts prognosis and immunotherapy in esophageal squamous cell cancer
Authors: Ren Qianhe , Zhang Pengpeng , Zhang Xiao , Feng Yanlong , Li Long , Lin Haoran , Yu Yue 
Published: May 2023

BackgroundCurrent paradigms of anti-tumor therapies are not qualified to evacuate the malignancy ascribing to cancer stroma’s functions in accelerating tumor relapse and therapeutic resistance. Cancer-associated fibroblasts (CAFs) has been identified significantly correlated with tumor progression and therapy resistance. Thus, we aimed to probe into the CAFs characteristics in esophageal squamous cancer (ESCC) and construct a risk signature based on CAFs to predict the prognosis of ESCC patients.MethodsThe GEO database provided the single-cell RNA sequencing (scRNA-seq) data. The GEO and TCGA databases were used to obtain bulk RNA-seq data and microarray data of ESCC, respectively. CAF clusters were identified from the scRNA-seq data using the Seurat R package. CAF-related prognostic genes were subsequently identified using univariate Cox regression analysis. A risk signature based on CAF-related prognostic genes was constructed using Lasso regression. Then, a nomogram model based on clinicopathological characteristics and the risk signature was developed. Consensus clustering was conducted to explore the heterogeneity of ESCC. Finally, PCR was utilized to validate the functions that hub genes play on ESCC.ResultsSix CAF clusters were identified in ESCC based on scRNA-seq data, three of which had prognostic associations. A total of 642 genes were found to be significantly correlated with CAF clusters from a pool of 17080 DEGs, and 9 genes were selected to generate a risk signature, which were mainly involved in 10 pathways such as NRF1, MYC, and TGF-Beta. The risk signature was significantly correlated with stromal and immune scores, as well as some immune cells. Multivariate analysis demonstrated that the risk signature was an independent prognostic factor for ESCC, and its potential in predicting immunotherapeutic outcomes was confirmed. A novel nomogram integrating the CAF-based risk signature and clinical stage was developed, which exhibited favorable predictability and reliability for ESCC prognosis prediction. The consensus clustering analysis further confirmed the heterogeneity of ESCC.ConclusionThe prognosis of ESCC can be effectively predicted by CAF-based risk signatures, and a comprehensive characterization of the CAF signature of ESCC may aid in interpreting the response of ESCC to immunotherapy and offer new strategies for cancer treatment.
Understanding the breeding phenology of anadromous fish Tenualosa ilisha (Hamilton, 1822) in relation to climatic variables in Brahmaputra River, India
Authors: Borah Simanku , Sahoo Amiya Kumar , Gopinathapillai Vaisakh , Meena Dharmendra Kumar , Jaiswar Ashok Kumar , Deshmukhe Geetanjali , Yadav Anil Kumar , Gogoi Pranab , Mohanty Debasmita , Ramteke Karankumar , Bhattacharjya Birendra Kumar , Das Basanta Kumar 
Published: May 2023

The Hilsa, Tenualosa ilisha, commands a very high value as food fish. The present study was carried out to understand the breeding phenology of T. ilisha in relation to climatic variables. Monthly fish samples were collected from two landing centres, namely, Uzanbazar (Guwahati) and Shri Ramghat, Dhubri, of River Brahmaputra during May 2018 to April 2019. The assessment of gonadosomatic index (GSI) of T. ilisha revealed higher GSI values during October to February, and showed temporal variations with respect to sex. In males, highest GSI value was observed in the month January followed by February, whereas in females, GSI value was found to be highest in November followed by October. GSI (pooled) value was negatively correlated with air temperature, indicating vulnerability of the species to climate change. The highest percentage of mature males was observed during October–February, and mature females during October–December. The length at first maturity was recorded to be 290 mm for female(s) and 259 mm for male(s). The absolute fecundity ranged from 103,164 to 583,456 ova for fishes in the size range of 229–403 mm, with an average of 250,532 ova per female. Relative fecundity was found to range from 306 to 1096 ova per gram body weight, with an average of 791 ova per gram body weight. The diameter of ova of the studied fishes ranged from 414.6 to 738.2 µm, with a mean value of 546.73 ± 7.18 µm. The percentage frequency distribution of mature ova shows a distinct single peak or mode. Sex ratio (male: female) was found to be 1:0.87, indicating dominance of males over females. The chi-square test on observed sex ratio against the hypothetical ratio of 1:1 did not reveal a significant difference (p &gt; 0.05). The findings of the present study can provide impetus toward successful management of this highly prized, transboundary, and migratory resource of River Brahmaputra, in the context of changing climate.
Mathematical prediction with pretreatment growth rate of metastatic cancer on outcomes: implications for the characterization of oligometastatic disease
Authors: Shin Yerim , Chang Jee Suk , Kim Yeseul , Shin Sang Joon , Kim Jina , Kim Tae Hyung , Liu Mitchell , Olson Robert , Kim Jin Sung , Sung Wonmo 
Published: May 2023

BackgroundOligometastatic disease (OMD) represents an indolent cancer status characterized by slow tumor growth and limited metastatic potential. The use of local therapy in the management of the condition continues to rise. This study aimed to investigate the advantage of pretreatment tumor growth rate in addition to baseline disease burden in characterizing OMDs, generally defined by the presence of ≤ 5 metastatic lesions.MethodsThe study included patients with metastatic melanoma treated with pembrolizumab. Gross tumor volume of all metastases was contoured on imaging before (TP-1) and at the initiation of pembrolizumab (TP0). Pretreatment tumor growth rate was calculated by an exponential ordinary differential equation model using the sum of tumor volumes at TP-1 and TP0 and the time interval between TP-1. and TP0. Patients were divided into interquartile groups based on pretreatment growth rate. Overall survival, progression-free survival, and subsequent progression-free survival were the study outcomes.ResultsAt baseline, median cumulative volume and number of metastases were 28.4 cc (range, 0.4-1194.8 cc) and 7 (range, 1-73), respectively. The median interval between TP-1 and TP0 was -90 days and pretreatment tumor growth rate (×10-2 days-1) was median 4.71 (range -0.62 to 44.1). The slow-paced group (pretreatment tumor growth rate ≤ 7.6 ×10-2 days-1, the upper quartile) had a significantly higher overall survival rate, progression-free survival, and subsequent progression-free survival compared to those of the fast-paced group (pretreatment tumor growth rate &gt; 7.6 ×10-2 days-1). Notably, these differences were prominent in the subgroup with &gt;5 metastases.ConclusionPretreatment tumor growth rate is a novel prognostic metric associated with overall survival, progression-free survival, and subsequent progression-free survival among metastatic melanoma patients, especially patients with &gt;5 metastases. Future prospective studies should validate the advantage of disease growth rate plus disease burden in better defining OMDs.
Mineral Composition and Consumer Acceptability of Amaranthus Leaf Powder Supplemented Ujeqe for Improved Nutrition Security
Authors: Olusanya Ruth N. , Kolanisi Unathi , Ngobese Nomali Z. 
Published: May 2023

Malnutrition, especially micronutrient deficiency, is a widespread health challenge that predominantly affects young children, young ladies who are within the productive age, refugees, and older adults who reside in rural communities and informal settlements in underdeveloped and developing countries. Malnutrition is caused by consuming either too little or too much of one or more food nutrients. Additionally, monotonous dietary lifestyle, especially the over-reliance on staple foods, is identified among the top factors limiting many individuals’ intake of essential nutrients. Thus, enriching starchy and cereal-based staple foods including Ujeqe (steamed bread) with fruits and especially leafy vegetables is being suggested as a strategic medium for essential nutrient delivery to malnourished populations and especially the Ujeqe regular consumers. Amaranthus, called pigweed, has been rediscovered as a nutrient-dense multipurpose plant. The seed has been explored as a nutrient-enhancer in staple foods; however, the leaves are underutilized, especially in Ujeqe. This study aims to enhance the mineral content of Ujeqe. An integrated research approach was used where Amaranthus dubius was self-processed into leaf powder. Amaranthus leaf powder (ALP), and the ALP-supplemented wheat flour Ujeqe prototypes 0%, 2%, 4%, and 6% were investigated for their mineral composition. Sensory evaluations of enriched Ujeqe were conducted using 60 panelists on a five-point hedonic scale. Findings show that the moisture contents of the raw materials and the supplemented prototypes were low, indicating a good shelf life of the food ingredient before being used for Ujeqe development. Carbohydrates of raw materials ranged from 41.6–74.3%, fat ranged from 1.58–4.47%, ash ranged from 2.37–17.97%, and protein ranged from 11.96–31.56%. Additionally, fat, protein, and ash content had significant differences at (p < 0.05). The moisture content of enhanced Ujeqe was equally low, connoting keeping quality of the sample. The increase concentration of ALP led to an enriched Ujeqe especially in the ash and protein content. Similarly, calcium, copper, potassium, phosphorus, manganese, and iron content were significantly influenced at (p < 0.05); 2% ALP-supplemented Ujeqe was the most acceptable prototype as the control sample, 6% was the least preferred prototype. Although ALP dubius can enrich staple foods including (Ujeqe), this study declared that higher addition of ALP dubius leads to low consumer acceptability rate of the Ujeqe, which is not statistically significant. Amaranthus is an economical source of fiber, which was not investigated in the study. Therefore, further studies can explore the fiber content of the ALP-supplemented Ujeqe.
MATERIAL DIDÁTICO COMO INSTRUMENTO MEDIADOR NA ATIVIDADE DE ENSINO DO CONCEITO DE FRAÇÃO
Authors: Romeiro Iraji de Oliveira , Moretti Vanessa Dias 
Published: May 2023

Este texto[1] apresenta parte dos resultados de uma pesquisa de mestrado fundamentada na perspectiva Histórico-Cultural, tendo como objetivo verificar como o movimento do pensamento teórico do professor de matemática sobre o conceito de fração pode impactar na escolha, adequação ou utilização dos materiais didáticos oficiais da rede pública de educação do Estado de São Paulo. O método utilizado para verificar o movimento do pensamento do professor se pautou no materialismo histórico dialético materializado por meio de um experimento formativo no formato de uma formação de professores de matemática da rede pública estadual de São Paulo, entendendo a formação docente como uma relação dialética entre a atividade de ensino e a atividade de aprendizagem. Para estruturar a formação de professores tomamos como referência o conceito de Atividade Orientadora de Ensino. Como resultados dos dados da realidade apreendida identificamos que o professor, ao se deparar com as situações desencadeadoras de aprendizagem que permitam a revelação das relações histórico-lógicas do conceito de fração permite um movimento em direção do pensamento teórico sobre frações. Com isso, os docentes analisam de forma intencional e crítica os materiais didáticos, transformando o significado e o sentido sobre desses materiais na prática pedagógica. Concluímos que, a partir do desenvolvimento do pensamento teórico, o professor tomou os materiais didáticos oficiais como instrumento mediador da sua atividade de ensino.

[1]Artigo ampliado a partir de trabalho apresentado no XIII ENEM 2019, Cuiabá/MT/Brasil.

[1]Artigo ampliado a partir de trabalho apresentado no XIII ENEM 2019, Cuiabá/MT/Brasil.
Distribusi Muatan Kapal Trammel Net di Pelabuhan Perikanan Samudera Cilacap, Jawa Tengah
Authors: Istiqomah Siti , Iskandar Budhi Hascaryo , Simbolon Domu , Yuwandana Dwi Putra , Novita Yopi 
Published: May 2023

Perairan Cilacap seringkali mengalami kondisi yang ekstrim dengan gelombang yang cukup besar karena perairan ini berhubungan langsung dengan Samudera Hindia. Kondisi perairan ini akan berdampak terhadap peristiwa kecelakaan kapal trammel net di sekitar perairan Cilacap. Kecelakaan kapal sebagai akibat faktor lingkungan ini memang sulit dikendalikan, akan tetapi faktor teknis seperti tata letak muatan yang mempengaruhi keseimbangan kapal trammel net dapat diperbaiki. Tujuan penelitian ini mengidentifikasi tata letak muatan pada kapal trammel net di PPS Cilacap, menentukan pengaruh penempatan muatan terhadap keberadaan titik berat kapal dan memberikan rekomendasi distribusi muatan yang baik. Analisis yang digunakan yaitu analisis tata letak muatan pada kapal trammel net di PPS Cilacap, analisis pengaruh penempatan muatan terhadap keberadaan titik berat kapal, dan rekomendasi distribusi muatan yang baik. Hasil penelitian menunjukkan bahwa distribusi atau tata letak muatan pada kapal trammel net di PPS Cilacap sebagian besar berada di atas lantai dek kapal. Nilai titik berat secara vertikal pada semua kondisi ketiga kapal yang diteliti tidak jauh berbeda karena adanya kompensasi dari hasil tangkapan dan perbekalan sedangkan titik berat secara horizontal sebagian besar ke arah haluan kapal. Penempatan muatan di bawah dek dan mendekati midship dapat meningkatkan keseimbangan kapal. Oleh karena itu, penempatan muatan berlebih di atas dek kapal sebaiknya dihindari. Berdasarakan analisis nilai KG pada 3 kondisi distribusi muatan, kapal I memiliki kondisi stabilitas lebih baik dibandingkan dengan kapal lainnya.
Filogeografi Trigonopoma gracile dari Sungai Gedong, Bangka dan Catatan di Paparan Sunda berdasarkan gen COI
Authors: Ardiansyah Kurniawan  , Apriyanti Rina , Safitri Alya Maisan , Almaghribi Siti Puan NIK , Syarif Ahmad Fahrul , Kurniawan Andri 
Published: May 2023

Trigonopoma gracile merupakan salah satu speies ikan air tawar yang ditemukan hidup di perairan Pulau Bangka yang terdampak penambangan timah. Perlu dilakukan kajian terkait hubungan genetik ikan ini dengan wilayah lainnya di paparan Sunda. Sampel ikan dari Sungai Gedong, Bangka diidentifikasi karakteristik molekulernya dengan menggunakan gen COI (sitokrom oksidase I) dan dibandingkan dengan temuan di wilayah Sundaland lainnya. Trigonopoma gracile dari Sungai Gedong, Bangka memiliki hubungan yang dekat dengan ikan T. gracile dari Kalimantan Barat, Serawak, Belitung, dan Sempan, Bangka dengan jarak antara 0,93 – 1,89 %, namun berbeda kluster dengan Selangor, Bangka Selatan, Kemuja-Bangka, Trengganu,dan Serawak dengan jarak genetik 3,69 – 5,44 %. Hubungan antar pulau pada T. gracile diprediksi terjadi akibat hubungan sunga purba Sunda Utara dimasa lampau. Variasi genetik yang muncul di Pulau Bangka dimungkinkan sebagai adaptasi terhadap kondisi lingkungannya.