bluuebunny/crossref_metadata_2025_split huggingface dataset:
Intracorporeal Anastomosis and ERAS Program: The Winning Combination of Optimized Postoperative Outcomes After Laparoscopic Right Colectomy
Authors: Meillat Hélène , Du Castel Héloise , Aubert Mathilde , Brun Clément , de Chaisemartin Cécile , Faucher Marion , Lelong Bernard 
Published: July 2023

Background:
Laparoscopic technique and the enhanced recovery after surgery (ERAS) protocol have become the standard of care in patients undergoing right colon cancer surgery, reducing hospital stay and postoperative morbidity. However, the optimal anastomosis technique [intracorporeal anastomosis (ICA) or extracorporeal anastomosis (ECA)] remains debated. This study aimed to determine the optimal perioperative approach (ICA vs. ECA combined with the ERAS program vs. standard care) for patients undergoing laparoscopic right colectomy for cancer.

Materials and Methods:
This single-center, retrospective study evaluated all consecutive patients who underwent laparoscopic right hemicolectomy for cancer between January 2012 and June 2021. Patient data were analyzed in 4 treatment groups according to the type of anastomosis and the care protocol. The primary endpoint was total postoperative hospital stay (THS). The secondary endpoints were recovery time and overall morbidity rate.

Results:
The baseline characteristics of the 373 included patients were comparable between the 4 groups. THS was significantly shorter in the ICA/ERAS group than in the ECA/standard and ICA/standard groups (P<0.01), but there was no significant difference between the ICA/ERAS and ECA/ERAS groups (5.7 vs. 6.4 d; P=0.17). Recovery time was significantly shorter in the ICA/ERAS group (P<0.01). The overall morbidity rate, including anastomotic morbidity, was comparable among the 4 groups. Regression analysis showed that ICA and the ERAS program were both predictive factors to reduce the THS and recovery time.

Conclusions:
ICA combined with the ERAS program achieves the best outcomes and thus seems to be the optimal approach for the management of patients undergoing laparoscopic right colectomy for cancer.
Transvaginal Natural Orifice Specimen Extraction (NOSE) in Laparoscopic Colorectal Cancer Surgery With New Insights on Technique and Patient Selection
Authors: Seow-En Isaac , Khor Shao Nan , Koo Chee Hoe , Wee Ian Jun Yan , Tan Emile Kwong-Wei 
Published: July 2023

Background:
Following laparoscopic colorectal surgery, transabdominal specimen extraction requires a mini-laparotomy or Pfannenstiel incision, associated with increased postoperative pain and wound complications. The vagina has several unique properties that make natural orifice specimen extraction (NOSE) ideal. We report our experience with transvaginal NOSE for colorectal cancer surgery.

Materials and Methods:
A transvaginal sizer allows the posterior vagina to be incised under tension. A transverse or cruciate incision is made, followed by the insertion of a double-ring wound protector. The external ring is opened against the perineum to shorten the length of the conduit for specimen delivery. Vaginotomy closure is performed via laparoscopy using a barbed suture.

Results:
Seventeen consecutive female patients underwent elective colorectal cancer surgery with attempted transvaginal NOSE. Median age and body mass index was 67 (range: 50 to 82) years and 26.5 (range: 19.7 to 35.8) kg/m2, respectively. Fourteen patients (82%) underwent left-sided resections and 3 (18%) underwent right-sided resections. Median operating time, blood loss, and length of hospital stay was 245 (range: 155 to 360) minutes, 30 (range: 10 to 500) mL, and 3 (range: 2 to 9) days, respectively. Transvaginal extraction was unsuccessful in 1 (6%) patient. Two (12%) patients experienced early postoperative morbidity, neither attributable to the extraction procedure. Median tumor circumferential diameter was 3.3 (range: 2.2 to 7.0) cm. Median follow-up duration was 17 (range: 8 to 27) months. There was no instance of sexual dysfunction.

Conclusions:
Transvaginal NOSE for colorectal cancer surgery is feasible and safe in selected patients. Overall specimen diameter, inclusive of tumor and mesentery, relative to pelvic outlet and conduit diameter is the most important consideration for transvaginal NOSE.
Balloon-occluded Esophageal Varices Obliteration Versus Ligation for the Management of F2 Esophageal Varices: A Randomized Controlled Trial
Authors: Mei Xuecan , Zhang Qianqian , Zhang Fuming , Jin Jing , Zeng Zhuang , Kong Derun 
Published: July 2023

Aim:
To compare balloon-occluded esophageal varices obliteration (BEVO) with esophageal variceal ligation (EVL) in the management of cirrhotic patients with type F2 esophageal varices (F2-EVs).

Materials and methods:
A total of 157 patients with F2-EVs were randomly assigned to either BEVO (n=79) or EVL (n=78) group in the prospective study between July 2021 and December 2021. Primary outcomes included recurrence and eradication rates. Secondary outcomes included rebleeding rate, and procedural complications.

Results:
The recurrence rate of EVs was notably lower in the BEVO group than in the EVL group (3.80% vs. 21.79%; P=0.001). The rate of complete eradication in the BEVO group was significantly higher than that of the EVL group (96.20% vs. 74.36%; P<0.001). The incidence of rebleeding in the BEVO group was markedly lower than that of the EVL group (7.59% vs. 20.51%; P=0.02). There was a higher incidence of transient dysphagia in the EVL group than in the BEVO group (10.26% vs. 1.27%; P=0.015).

Conclusions:
BEVO exerted an effective treatment option for F2-EVs.
Artificial Intelligence Helps to Predict Recurrence and Mortality for Prostate Cancer Using Histology Images
Authors: Eminaga Okyaz , Saad Fred , Tian Zhe , Wolffgang Ulrich , Karakiewicz Pierre I. , Ouellet Véronique , Azzi Feryel , Spieker Tilmann , Helmke Burkhard M. , Graefen Markus , Jiang Xiaoyi , Xing Lei , Witt Jorn H. , Trudel Dominique , Leyh-Bannurah Sami-Ramzi 
Published: July 2023

AbstractBesides grading, deep learning could improve expert consensus to predict prostate cancer (PCa) recurrence. We developed a novel PCa recurrence prediction system based on artificial intelligence (AI). We validated it using multi-institutional and international datasets comprising 2,647 PCa patients with at least a 10-year follow-up. Survival analyses were performed and goodness-of-fit of multivariate models was evaluated using partial likelihood ratio tests, Akaike’s test, or Bayesian information criteria to determine the superiority of our system over existing grading systems. Comprehensive survival analyses demonstrated the effectiveness of our AI- system in categorizing PCa into four distinct risk groups. The system was independent and superior to the existing five grade groups for malignancies. A high consensus level was observed among five blinded genitourinary pathology experts in ranking images according to our prediction system. Therefore, AI may help develop an accurate and clinically interpretable PCa recurrence prediction system, facilitating informed decision-making for PCa patients.
A new paradigm of intracrine free fatty acid receptor 4 signaling at lipid droplets
Authors: Tripp Emma , O’Brien Shannon L. , Smith Gabrielle , Boufersaoui Adam , Roberts Jennie , Pike Jeremy , Correia Joao , Miljus Tamara , Tennant Daniel A. , Hudson Brian D. , Milligan Graeme , Gerhart-Hines Zachary , Schwartz Thue W. , Calebiro Davide 
Published: July 2023

ABSTRACTG protein-coupled receptors (GPCRs), once thought to be active exclusively at the plasma membrane, have been shown to signal from multiple intracellular membrane compartments, including endosomes and the Golgi. However, the potential occurrence and functional relevance of intracellular signaling for the emerging family of metabolite-sensing GPCRs is largely unknown. Here, we used live-cell imaging, bioluminescence resonance energy transfer (BRET) measurements, and functional readouts to investigate signal compartmentalization of the free fatty acid receptor 4 (FFA4), a prototypical metabolite-sensing GPCR that is activated by medium- and long-chain free fatty acids (FFAs). Unexpectedly, we show that FFA4 largely resides on intracellular membranes that are intimately associated with lipid droplets in adipocytes. Upon lipolysis induction, the released FFAs rapidly bind to and activate this intracellular pool of FFA4, leading to local Gi/ocoupling and inhibition of cAMP production in the vicinity of lipid droplets. This provides a spatiotemporally confined negative feedback mechanism allowing individual lipid droplets to rapidly adjust their lipolysis rate. Our results reveal a novel ‘intracrine’ signaling modality by a prototypical metabolite-sensing GPCR and identify a new lipid-droplet-associated signaling hub implicated in the rapid regulation of lipid metabolism, with important implications for adipocyte physiology and pharmacology.
Spring reproductive success influences autumnal malarial load in a passerine bird
Authors: Pigeault Romain , Cozzarolo Camille-Sophie , Wassef Jérôme , Gremion Jérémy , Bastardot Marc , Glaizot Olivier , Christe Philippe 
Published: July 2023

AbstractAlthough avian haemosporidian parasites are widely used as model organisms to study fundamental questions in evolutionary and behavorial ecology of host-parasite interactions, some of their basic characteristics, such as seasonal variations in within-host density, are still mostly unknown. In addition, their interplay with host reproductive success in the wild seems to depend on the interaction of many factors, starting with host and parasite species and the temporal scale under study. Here, we monitored the parasitemia of two haemosporidian parasites –Plasmodium relictum(lineage SGS1) andP. homonucleophilum(lineage SW2) – in two wild populations of great tits (Parus major) in Switzerland over three years, to characterize their dynamics. We also collected data on birds’ reproductive output – laying date, clutch size, fledging success – to determine whether they were associated with parasitemia before (winter), during (spring) and after (autumn) breeding season. Parasitemia of both species dramatically increased in spring, in a way that was correlated to parasitemia in winter. Parasitemia before and during breeding season did not explain reproductive success. However, the birds which fledged the more chicks had higher parasitemia in autumn, which was not associated with their parasitemia in previous spring. Our results tend to indicate that high haemosporidian parasite loads do not impair reproduction in great tits, but high resource allocation into reproduction can leave birds less able to maintain low parasitemia over the following months.
Clinical prediction of wound re-epithelisation outcomes in non-severe burn injury using the plasma lipidome
Authors: Ryan Monique J. , Raby Edward , Masuda Reika , Lodge Samantha , Nitschke Philipp , Maker Garth L. , Wist Julien , Fear Mark W. , Holmes Elaine , Nicholson Jeremy K. , Gray Nicola , Whiley Luke , Wood Fiona M. 
Published: July 2023

AbstractWhilst wound repair in severe burns has received substantial research attention, non-severe burns (<20% total body surface area) remain relatively understudied, despite causing considerable physiological impact and constituting most of the hospital admissions for burns. Early prediction of healing outcomes would decrease financial and patient burden, and aid in preventing long-term complications from poor wound healing. Lipids have been implicated in inflammation and tissue repair and may play essential roles in burn wound healing. In this study, plasma samples were collected from 20 non-severe burn patients over 6 weeks from admission, including surgery, and analysed by liquid chromatography-tandem mass spectrometry and nuclear magnetic resonance spectroscopy to detect 850 lipids and 112 lipoproteins. Orthogonal projections to latent structures-discriminant analysis was performed to identify changes associated with re-epithelialisation and delayed re-epithelisation.We demonstrated that the lipid and lipoprotein profiles at admission could predict re-epithelisation outcomes at 2 weeks post-surgery, and that these discriminatory profiles were maintained up to 6 weeks post-burn. Inflammatory markers GlycB and C-reactive protein indicated divergent systemic responses to the burn injury at admission. Triacylglycerols, diacylglycerols and low-density lipoprotein subfractions were associated with delayed wound closure (p-value <0.02, Cliff’s delta >0.7), whilst high-density lipoprotein subfractions, phosphatidylinositols, phosphatidylcholines, and phosphatidylserines were associated with re-epithelisation at 2 weeks post-surgery (p-value <0.01, Cliff’s delta <-0.7). Further model validation will potentially lead to personalised intervention strategies to reduce the risk of chronic complications post-burn injury.
Targeted Photoconvertible BODIPYs Based on Directed Photooxidation Induced Conversion for Applications in Photoconversion and Live Super Resolution Imaging
Authors: Saladin Lazare , Breton Victor , Lequeu Thibault , Didier Pascal , Danglot Lydia , Collot Mayeul 
Published: July 2023

ABSTRACTPhotomodulable fluorescent probes are drawing an increasing attention due to their applications in advanced bioimaging. Whereas photoconvertible probes can be advantageously used in tracking, photoswitchable probes constitute key tools for single molecule localization microscopy to perform super resolution imaging. Herein we shed light on a red and far-red BODIPY, namely BDP-576 and BDP-650 possessing both properties of conversion and switching. Our study demonstrates that theses pyrrolyl-BODIPYs respectively convert towards typical green- and red-emitting BODIPYs that are perfectly adapted to microscopy. We also showed that these pyrrolyl-BODIPYs undergo Directed Photooxidation Induced Conversion, a photoconversion mechanism that we recently introduced and where the pyrrole moiety plays a central role. These unique features were used to develop targeted photoconvertible probes towards different organelles or subcellular units (plasma membrane, mitochondria, nucleus, actin, Golgi apparatus,etc.) using chemical targeting moieties and Halo tag. We notably showed that BDP-650 could be used to track intracellular vesicles over more than 20 minutes in two color imaging with laser scanning confocal microscopy demonstrating its robustness. The switching properties of these photoconverters were studied at the single molecule level and were then successfully used in live Single Molecule Localization Microscopy in epithelial cells and neurons. Both membrane and mitochondria targeted probes could be used to decipher membrane 3D architecture and mitochondria dynamics at the nanoscale. This study builds a bridge between the photoconversion and photoswitching properties of probes undergoing directed photooxidation and shows the versatility and efficacy of this mechanism in live advanced imaging.
Earthworms and plants can decrease soil greenhouse gases emissions by modulating soil moisture fluctuations and soil macroporosity in a mesocosm experiment
Authors: Ganault Pierre , Nahmani Johanne , Capowiez Yvan , Fromin Nathalie , Shihan Ammar , Bertrand Isabelle , Buatois Bruno , Milcu Alexandru 
Published: July 2023

AbstractEarthworms can stimulate microbial activity and hence, greenhouse gas (GHG) emissions from soils. However, the extent of this effect in the presence of plants and soil moisture fluctuations, which are influenced by earthworm burrowing activity, remains uncertain. Here we report the effect of earthworms (without, anecic, endogeic, both) and plants (with, without) on GHG (CO2, N2O) emissions in a 3 month-greenhouse mesocosm experiment simulating a simplified agricultural context. The mesocosms allowed for water drainage at the bottom to account for the earthworm engineering effect on water flow during two drying-wetting cycles. N2O cumulative emissions were 34.6 and 44.8% lower when both earthworm species and only endogeic species were present, respectively, and 19.8% lower in presence of plants. The presence of the endogeic species alone or in combination with the anecic species slightly reduced CO2emissions by 5.9% and 11.4% respectively, and plants presence increased emissions by 6%. Earthworms, plants and soil water content interactively affected weekly N2O emissions, an effect controlled by increased soil dryness due to drainage via earthworm burrows and mesocosm evapotranspiration. Soil macroporosity (measured by X-ray tomography) was affected by earthworm species-specific burrowing activity. Both GHG emissions decreased with top soil macropore volume, presumably due to reduced moisture and microbial activity. N2O emissions decreased with macropore volume in the deepest layer, likely due to fewer anaerobic microsites. Our results indicate that, under experimental conditions allowing for plant and earthworm engineering effects on soil moisture, earthworms do not increase GHG emissions and that endogeic earthworms may even reduce N2O emissions.
The C-terminus of α-Synuclein regulates its dynamic cellular internalization by Neurexin 1β
Authors: Birol Melissa , Douzoglou Muñoz Isabella Ioana , Rhoades Elizabeth 
Published: July 2023

AbstractThe aggregation of the disordered neuronal protein, α-Synuclein (αS), is the primary pathological feature of Parkinson’s disease. Current hypotheses favor cell-to-cell spread of αS species as underlying disease progression, driving interest in identifying the molecular and cellular species involved in cellular internalization of αS. Prior work from our lab identified the chemically specific interaction between αS and the pre-synaptic adhesion protein neurexin 1β (N1β) to be capable of driving cellular internalization of both monomer and aggregated forms of αS. Here we explore the physical basis of N1β-driven internalization of αS. Specifically, we show that spontaneous internalization of αS by SH-SY5Y and HEK293 cells expressing N1β requires essentially all of the membrane-binding domain of αS; αS constructs truncated beyond residue 90 bind to N1β in the plasma membrane of HEK cells, but are not internalized. Interestingly, prior to internalization, αS and N1β co-diffuse rapidly in the plasma membrane. αS constructs that are not internalized show very slow mobility themselves, as well as slow N1β diffusion. Finally, we find that truncated αS is capable of blocking internalization of full-length αS. Our results draw attention to the potential therapeutic value of blocking αS-N1β interactions.
Single tyrosine mutation in VE-cadherin modulates gene lung expressions: evidence for FOXF1 mediated S1PR1 upregulation to stabilize vessels in mice
Authors: Garnier Olivia , Jeanneret Florian , Durand Aude , Fertin Arnold , Martin Donald , Berndt Sarah , Carpentier Gilles , Battail Christophe , Vilgrain Isabelle 
Published: July 2023

ABSTRACTRationalePhosphorylation-dephosphorylation are processes involved in the adhesion of endothelial cells (ECs) to maintain vascular integrity in adults. VE-cadherin is a target for Src-mediated Y685phosphorylation, identified in highly vascularized human glioblastoma where it is involved in the abnormal feature of tumor blood vessels.ObjectiveWe aimed at understanding the molecular mechanisms through which Y685F-VE-cadherin triggers S1PR1 gene expression and stabilizes lung vessels in adult mice.Methods and ResultsWe compared lung ECs from a knock-in (KI) mouse carrying a point mutation in VE-cadherin (Tyr 685 to Phe) to Wild type. Analysis of EC parameters showed a difference in the migratory rate was between ECs from KI (22.45% ± 5.207) and WT (13.24% ± 5.17) (p-value=0.034). The direct adhesion of ECs from KI mice to fibronectin was significantly higher (37.625 ± 9.23) than that of the WT (26.8 ± 3.258, p-value=0.012). In the fibrin bead assay, ECs from KI showed a weaker angiogenic response. The transcriptome of mutated ECs showed that 884 genes were dysregulated of which 766 genes were downregulated and 118 genes were upregulated. The Gene Ontology Enrichment showed that most of the genes were related to cell-cell adhesion and angiogenesis. Focusing on angiogenic genes, we found that Sphingosine-1-phosphate-receptor was a gene upregulated in mutated ECs which was confirmed by RT-PCR and westernblotting. Mechanistically, chromatin immunoprecipitation assay (CHIPS) demonstrated that FOXF1 directly bound to the S1pr1 promoter 7 fold greater than WT. As a consequence, VE-cadherin at the membrane was higher in the mutant vs WT (100 ± 6.52 for WT vs 189.7 ± 21.06 for KI (p-value 0.0001). Finally, lung morphometric analysis showed less vessels and vascular remodeling with no fibrosis in mutated mice.ConclusionsThese data extend our knowledge on pY-VE-cadherin mediated pathological angiogenesis and provide new therapeutic opportunities to vascular normalization through pharmacological inhibition of the Y685-VE-cadherin phosphorylation.
Birth of an organelle: molecular mechanism of lipid droplet biogenesis
Authors: Nieto Vincent , Crowley Jackson , Santos Denys , Monticelli Luca 
Published: July 2023

AbstractLipid droplets (LDs) are cellular organelles regulating energy and lipid metabolism. Generated in the endoplasmic reticulum (ER) by phase separation of neutral lipids (e.g., triglycerides), nascent LDs resemble lens-shaped blisters, grow into spherical droplets, and eventually emerge from the ER membrane, generally towards the cytosol – a process known as budding. Images of both nascent and mature LDs are available, but the mechanism of biogenesis has never been observed experimentally.Here we identify the mechanism of the initial steps in LD biogenesis using computer simulations at the molecular level, emulating LD growth in ER-mimicking membranes. We find that LDs bud towards the cytosol only when sufficient asymmetry is generated between the two membrane leaflets: the budding transition is independent of membrane morphology, lipid composition, and LD size. Seipin, a protein essential for correct LD biogenesis, isper senot sufficient to promote budding, but it stabilizes LD-ER contact sites. Localization of triglyceride synthesis in the proximity of seipin is necessary to avoid nucleation of multiple LDs – a possible cause of aberrant phenotypes. In contrast, localization of phospholipid synthesis has no effect on the mechanism of budding. Our new methodology paves the way to simulations of organelle and cell biogenesis.