bluuebunny/crossref_metadata_2025_split huggingface dataset:
Neuroeducación: una mirada para potenciar el aprendizaje de la física en estudiantes de segundo año de bachillerato
Authors: Bozada Morales Elva Alexandra , Suárez Lombeida María Magdalena , Mesa Villavicencio Paulina 
Published: January 2025

Introducción: El artículo aborda la influencia de la neuroeducación en el aprendizaje de la física en estudiantes de segundo año de Bachillerato. Objetivo: identificar estrategias didácticas que promuevan un aprendizaje significativo de la física mediante la integración de conceptos de la neuroeducación. Metodología: La investigación se desarrolló mediante una metodología mixta que incluyó la aplicación de encuestas a 37 estudiantes y entrevistas a docentes que imparten la asignatura de física, con la finalidad de conocer las percepciones sobre las prácticas pedagógicas, y la motivación en el aula. Este enfoque permitió recopilar información cualitativa y cuantitativa para fundamentar los hallazgos. Resultados: mostraron puntos favorables y no favorables. Lo favorable es la percepción de los estudiantes de la enseñanza de la física, y la evidencia no favorable es la necesidad de estrategias que estimulen la memoria, la atención y la motivación en los estudiantes. Se diseñó una propuesta educativa basada en ejercicios prácticos y trabajo experimental en principios neuro educativos, fomentando el aprendizaje experiencial y colaborativo. Estas actividades no solo buscan fomentar el aprendizaje significativo, sino también desarrollar habilidades como el pensamiento crítico y la resolución de problemas. Conclusión: En conclusión, la neuroeducación puede ser una herramienta clave para mejorar la calidad educativa, promoviendo la comprensión profunda y el desarrollo integral de los estudiantes. Área de estudio general: Educación. Área de estudio específica: Neuroeducación. Tipo de estudio: Artículos originales.
Elaboración de una guía de ejercicios terapéuticos en la displasia de la cadera en la clase de educación física
Authors: Espinoza Acosta José Luis , Coello Sánchez Ángel Miguel , Maqueira Caraballo Giseya de la Caridad , Rodríguez Vargas Antonio Ricardo 
Published: January 2025

Introducción: la displasia de cadera (DDC) se trata de una afección en la que la articulación de la cadera no se desarrolla adecuadamente, impidiendo una movilidad articular poco estable y equilibrada para llevar una vida saludable. El presente artículo realizará una guía de ejercicios físicos para el estudiante que presenta esta patología permitiéndole la estimulación y el desarrollo de habilidades en la clase de educación física (EF). Objetivo: el objetivo de este artículo es elaborar una guía de ejercicios terapéuticos en la DDC en la clase de EF para tratar su condición, preservar o mejorar el desarrollo psicomotor y funcional que puede verse afectado por esta patología, abordando aspectos prácticos y metodológicos que ayudan a tener una ventaja favorable para el estudiante a nivel educativo. Metodología: En este caso se aplicará el método de diagnóstico con una entrevista y control observacional para investigar factores de riesgo aplicando el método cualitativo, en las investigaciones de otros estudios se considerará realizar análisis biomecánicos no experimental para comprender mejor las alteraciones en la articulación de la cadera. Resultados: una guía de ejercicios terapéuticos en la DDC en la clase de EF tiene un impacto remediar a nivel educativo favorable de ventajas para el estudiante y no a nivel clínico. Conclusiones: estos ejercicios han demostrado la efectividad en su proceso, como la estudiante que presenta esta patología ha comprobado, que realizando actividad física conlleva a una vida saludable presentando una movilidad articular pélvica funcional a un porcentaje más elevado de lo que ella presentaba al inicio. Área general del conocimiento: Educación física. Área específica del conocimiento: Fisioterapia. Tipo de estudio: Artículos originales.
SOSIALISASI PENGGUNAAN GOOGLE DRIVE DALAM PENYIMPANAN DOKUMEN PADA PEMBELAJARAN MATA KULIAH MANUSIA DAN KEBUDAYAAN OLEH MAHASISWA FAKULTAS ILMU BUDAYA UNIVERSITAS HALU OLEO
Authors: Wulandari Kiki Reski , Marhini La Ode , Rihu Agus , Samiel Edy , Ustianti Ustianti 
Published: January 2025

Kegiatan pengabdian ini bertujuan untuk memberikan pengetahuan kepada mahasiswa sehingga mereka memiliki penguasaan ilmu pengetahuan dan teknologi (IPTEK) khususnya dalam hal proses digitalisasi penggunaan Google Drive pada penyimpanan dokumen. Dengan adanya kegiatan pengabdian ini diharapkan mahasiswa dapat memahami dan menguasai proses penyimpanan data elektronik sehingga ke depannya mahasiswa akan memiliki pemahaman dan menggunakan Google Drive sebagai tempat penyimpan dokumen penting dan dokumen dengan kapasitas yang besar. Metode pengabdian yang digunakan dalam pelatihan ini yaitu dengan sistem pembelajaran tatap muka yang disertai materi pelatihan yang dianggap paling mutakhir dalam perkembangan ilmu pengetahuan dan teknologi (IPTEK). Proses pelaksanaan pengabdian ini melibatkan mahasiswa secara langsung pada proses digitalisasi penggunaan Google Drive dalam penyimpanan dokumen elektronik sehingga mereka dipersiapkan menjadi lulusan yang kreatif, inovatif dan berkualitas.
Association between D-dimer to lymphocyte ratio and in hospital all-cause mortality in elderly patients with sepsis: a cohort of 1123 patients
Authors: Long Xinguang , Hu Zhenkui , Song Chao , Zhang Jinhui 
Published: January 2025

BackgroundThe D-dimer to lymphocyte ratio (DLR), a novel inflammatory biomarker, had been shown to be related to adverse outcomes in patients with various diseases. However, there was limited research on the relationship between the DLR and adverse outcomes in patients with infectious diseases, particularly those with sepsis. Therefore, this study aimed to explore the association between the DLR and in hospital all-cause mortality in elderly patients with sepsis.MethodsA total of 1123 patients admitted in intensive care unit (ICU) were included in this study. The patients were categorized into quartiles (Q1-Q4) based on their DLR values. The primary outcomes included hospital mortality and ICU mortality. Kaplan-Meier analysis was conducted to compare all-cause mortality among the four DLR groups. The association between DLR and all-cause mortality in patients with sepsis was further elucidated using the receiver operating characteristic (ROC) curve and Cox proportional hazards regression analysis.ResultsThe study included participants with a median age of 75 (65-84) years, with 707 (63.0%) being male. The rates of hospital mortality and ICU mortality were 33.7% and 31.9%, respectively. Kaplan-Meier analysis highlighted a significantly increased risk of all-cause mortality among patients with elevated DLR values (log-rank p &lt; 0.001). ROC curve analyses revealed that DLR had a stronger ability to predict hospital mortality and ICU mortality in patients with sepsis than D-dimer or Lym. Multivariable Cox proportional hazards analyses revealed DLR as an independent predictor of hospital death [per 1 SD increase in DLR: HR (95% CI): 1.098 (1.020-1.181); p = 0.013] and ICU death [per 1 SD increase in DLR: HR (95% CI): 1.095 (1.017-1.180); p = 0.017] during the hospital stay.ConclusionsA higher DLR value was associated with hospital and ICU all-cause death in elderly patients with sepsis. This finding demonstrated that the DLR could be a convenient and useful prognostic marker for sepsis prognosis.
Silent cerebral lesions after catheter ablation for atrial fibrillation using cryoballoon, hotballoon, laserballoon and radiofrequency catheters: a Bayesian network meta-analysis
Authors: Zheng Tiantian , Kong Youjin , Wu Li , Wu Chenxia , Mao Wei , Zhou Xinbin 
Published: January 2025

BackgroundCatheter ablation (CA) is an effective therapeutic option for patients with symptomatic atrial fibrillation (AF). Previous studies have reported silent cerebral lesions (SCLs) detected by magnetic resonance imaging (MRI) after different CA techniques; however, the results were controversial. Therefore, we performed this network meta-analysis (NMA) to assess the incidence of SCLs after cryoballoon, hotballoon, laserballoon, and radiofrequency ablation (RFA).MethodsDatabases such as PubMed, Embase, and the Cochrane Library were searched systematically. Both pairwise meta-analysis (PMA) and NMA were conducted. The primary outcome was the incidence of new SCLs on MRI after CA procedures.ResultsNine studies were analyzed and 1,057 patients were enrolled. Laserballoon ablation (LBA) had a higher incidence of SCLs than cryoballoon ablation (CBA) [odds ratio [OR] = 1.86, 95% confidence interval [CI] 1.06–3.27, p = 0.032] in the PMA, while no significant difference was detected between the CA techniques according to the NMA. The surface under the cumulative ranking curve (SUCRA) values indicated that CBA may be the best therapeutic option (SUCRA = 81.1%). The NMA results demonstrated similar procedure-related complication rates and mean activated clotting time between CBA (SUCRA = 53.7%, 66.3%), hotballoon ablation (HBA) (SUCRA = 81.5%, 43.6%), LBA (SUCRA = 3.39%, 42.8%) and RFA (SUCRA = 61.3%, 47.3%). LBA therapy required significantly more procedure time than CBA [weighted mean difference (WMD) = 24.36 min, 95% CI 12.51–36.21 min, p = 0.00].ConclusionsCBA treatment had lower incidence of post-procedural SCLs and took less procedure time compared with LBA for patients with AF. The procedure-related complications were comparable between CBA, LBA, HBA and RFA.Systematic Review RegistrationPROSPERO, identifier (CRD42024511110).
Relationship between ferroptosis and healing of diabetic foot ulcer: a prospective clinical study
Authors: Jiang Qian , Tang Long , Xiao Shuao 
Published: January 2025

ObjectiveDiabetic foot ulcer (DFU) is one of the common complications in patients with diabetes mellitus (DM). In order to find a method to monitor and treat the refractory DFU, the ferroptosis level in DFU and traumatic wounds (TW) was monitored and the difference between them was analyzed. At the same time, this study further analyzed the correlation of ferroptosis levels with DM severity and DFU’s healing.MethodsA prospective cohort study was from January, 2021 to December, 2023 in the Second People’s Hospital of Gansu province, which included 59 patients with DFU and 42 patients with TW. We then used the kit to detect the indicators related to ferroptosis, including 4-Hydroxynonenal (4-HNE), Malondialdehyde (MDA) and reactive oxygen species (ROS), in the wound exudate of the two groups of patients.ResultsThe DFU group had higher ferroptosis level than the TW group (4-HNE: P = 0.003, MDA: P&lt;0.001, ROS: P&lt;0.001). The severity of diabetes was significantly associated with ferroptosis level in DFU patients(r = 0.936, P &lt;0.001). The results of multiple regression analysis showed that 4-HNE (β = -0.182, P = 0.008), MDA (β = -0.478, P &lt;0.001) and ROS (β = -0.394, P&lt;0.001) significantly negatively predicted the healing rate of DFU.ConclusionAs a new monitoring and therapeutic target, ferroptosis level plays an important role in predicting the healing rate of DFU and assisting clinical treatment decision-making.
Comparison of immune responses to SARS-CoV-2 spike following Omicron infection or Omicron BA.4/5 vaccination in kidney transplant recipients
Authors: Tometten Inga , Brandt Tobias , Schlotz Maike , Stumpf Ricarda , Landmann Sinje , Kantauskaite Marta , Lamberti Joshua , Hillebrandt Jonas , Müller Lisa , Kittel Margarethe , Ivens Katrin , Gruell Henning , Voges Anja , Schaal Heiner , Lübke Nadine , Königshausen Eva , Rump Lars Christian , Klein Florian , Stegbauer Johannes , Timm Jörg 
Published: January 2025

BackgroundThe emergence of novel SARS-CoV-2 variants challenges immunity, particularly among immunocompromised kidney transplant recipients (KTRs). To address this, vaccines have been adjusted to circulating variants. Despite intensive vaccination efforts, SARS-CoV-2 infections surged among KTRs during the Omicron wave, enabling a direct comparison of variant-specific immunity following-vaccination against Omicron BA.4/5 or Omicron infection in KTRs.Methods98 SARS-CoV-2 naïve KTRs who had received four vaccine doses were studied. Before and after a 5th antigen exposure, either via the bivalent vaccine composed of ancestral SARS-CoV-2 and Omicron BA.4/5 (29 KTRs) or via natural infection with Omicron (38 BA.4/5, 31 BA.1/2), spike-specific T cells were quantified using Elispot and serum pseudovirus neutralizing activity was assessed against the ancestral Wuhan strain, BA.5 and XBB.1.5.ResultsCompared to BA.4/5 vaccination, spike-specific T-cell responses and neutralization activity were higher up to six months post-Omicron infection and reached levels similar to healthy controls. Vaccinated KTRs showed modestly boosted neutralization activity against the Wuhan strain and BA.5, but not XBB.1.5. Baseline immunity correlated with immune responses three months post-vaccination and post-infection, indicating a predictive value for peak immune responses. Tixagevimab/Cilgavimab treatment was associated with robust neutralization of the Wuhan strain, but ineffective against XBB.1.5.ConclusionThe BA.4/5 vaccine improved neutralizing activity against the BA.4/5 variant, but not against the subsequently circulating XBB.1.5 variant in KTRs. Conversely, omicron infection boosted T cells and humoral responses more effectively, showing efficacy against XBB.1.5. These findings suggest that infection-induced immunity associates with greater protection than vaccination against future variants in KTRs.
Fatty acid synthase inhibition improves hypertension-induced erectile dysfunction by suppressing oxidative stress and NLRP3 inflammasome-dependent pyroptosis through activating the Nrf2/HO-1 pathway
Authors: Luan Jiaochen , Yu Mengchi , Gu Qi , Zhou Xuan , Shao Yunqiang , Chen Tong , Zhang Jiayi , Zhu Zheng , Song Ninghong , Yang Jie 
Published: January 2025

BackgroundErectile dysfunction (ED) is a prevalent male sexual disorder, commonly associated with hypertension, though the underlying mechanisms remain poorly understood.ObjectiveThis study aims to explore the role of Fatty acid synthase (Fasn) in hypertension-induced ED and evaluate the therapeutic potential of the Fasn inhibitor C75.Materials and methodsErectile function was assessed by determining the intracavernous pressure/mean arterial pressure (ICP/MAP) ratio, followed by the collection of cavernous tissue for transcriptomic and non-targeted metabolomic analyses. In vitro, a concentration of 10-6 M angiotensin II (Ang II) was applied to rat aortic endothelial cells (RAOECs) to establish a model of hypertension. In vivo, spontaneously hypertensive rats (SHR) were randomly divided into two groups. The SHR+C75 group received intraperitoneal injections of C75 at a dose of 2 mg/kg once a week. After five weeks of treatment, the erectile function of the rats was assessed, and penile tissues were harvested for further analysis. Molecular and protein expression were assessed using Western blotting, qRT-PCR, immunofluorescence staining, and immunohistochemistry.ResultsThe SHR exhibited ED, indicated by reduced maximum ICP/MAP ratios. Histologically, corpus cavernosum tissue of SHR showed elevated fibrosis and endothelial dysfunction. Additionally, increased expression of the NLRP3 inflammasome, Caspase-1, GSDMD, and the pro-inflammatory cytokines IL-1β and IL-18 was observed. Multi-omics analysis revealed significant enrichment in lipid metabolic pathways, with Fasn identified as a hub gene. In vitro, siFasn and C75 enhanced antioxidant markers Nrf2 and HO-1, reduced ROS accumulation, and suppressed NLRP3 and GSDMD levels. In vivo, C75 treatment restored endothelial function and reversed erectile dysfunction, accompanied by decreased oxidative stress and pyroptosis in the penile corpus cavernosum.ConclusionThese findings suggest that Fasn inhibition may offer a promising therapeutic strategy for hypertension-induced ED by alleviating oxidative stress and suppressing NLRP3 inflammasome-dependent endothelial cell pyroptosis via activation of the Nrf2/HO-1 pathway.
Genetic variations suggests that Takifugu rubripes, T. chinensis, and T. pseudommus are the same species with a shared gene pool
Authors: Lee Ji Young , Kim Kun Hee , Lee Hyuk Je , Hwang Sun-Goo , Kang Tae Sun 
Published: January 2025

IntroductionPufferfish are valuable ingredients and important fishery resources in Asia. Among them, approximately 25 Takifugu species have undergone explosive speciation in the marine environments of East Asia. Specifically, T. rubripes, T. pseudommus, and T. chinensis are remarkably similar in appearance and genetics, and their classification remains controversial. Here, we provide comprehensive genetic and genomic evidence regarding their speciation and classification.MethodsWhole genome sequencing was performed on T. pseudommus, leading to the identification and development of 15 novel tandem simple sequence repeats (SSRs) from its genomic data. Genetic diversity, differentiation, and population structure among T. rubripes, T. pseudommus, T. chinensis, and T. xanthopterus were subsequently analyzed using a combination of these 15 SSR loci and mitochondrial cytochrome oxidase subunit I (CoI) and cytochrome b (CytB) gene sequences. Furthermore, genomic variations, including single nucleotide polymorphisms, insertions, and deletions, were identified by comparing the genome of T. pseudommus with the reference T. rubripes genome retrieved from the National Center for Biotechnology Information. These variations were screened using ENSEMBL annotation and gene ontology analysis, and their potential association with morphological differences, such as dorsal spot patterns, was evaluated using reference Takifugu specimens.Results and discussionPopulation genetic analysis of T. rubripes, T. pseudommus, and T. chinensis using the CoI and CytB genes, combined with the 15 SSR loci, revealed clustering into a single genetic group characterized by remarkably low genetic diversity (four haplotypes, with diversity values ranging from 0.0000 to 0.00065) and minimal pairwise differentiation (microsatellite-based FST values ranging from -0.0021 to 0.0075). Additionally, comparative genomic analysis between T. pseudommus and the reference T. rubripes genome did not identify genetic variations that could directly explain the observed morphological differences among the species. These findings strongly suggest that T. rubripes, T. pseudommus, and T. chinensis represent a single species sharing a common genetic pool.
Transcutaneous vagus nerve stimulation for Parkinson’s disease: a systematic review and meta-analysis
Authors: Shan Jiatong , Li Zehong , Ji Minxiu , Zhang Miao , Zhang Caidi , Zhu Yikang , Feng Zhen 
Published: January 2025

BackgroundTranscutaneous vagus nerve stimulation (tVNS) has emerged as a novel noninvasive adjunct therapy for advanced Parkinson’s disease (PD), yet no quantitative analysis had been conducted to assess its therapeutic effect.ObjectivesThis review aimed to investigate the efficacy of tVNS on motor function, other potential clinical targets and its safety in various treatment conditions.MethodsWe searched six databases for randomized controlled trials (RCTs) that involved treating PD patients with tVNS. Primary outcome was motor functions, including severity of motor signs, functional mobility and balance, and gait parameters. Secondary outcomes were cognition, emotion, sleep related impairments, patient reported non-motor outcomes, and any adverse events. All outcomes were classified and analyzed according to the treatment duration and medication condition of an included study. Risk of bias was evaluated by referencing Cochrane risk of bias tool 1.0. Data was analyzed by Revman 5.4.Results6 RCTs with 176 PD patient were included. Several motor functions and non-motor functions measured during on-medication condition (severity of motor signs −0.48 [95% CI −0.93, −0.04], gait −0.48 [95% CI −0.85, −0.1], patients reported non-motor outcomes −0.4 [95% CI −0.78, −0.03]), improved significantly. However, verbal fluency, sleep-related impairment, and fatigue were negatively impacted by tVNS during on-medication condition. No distinct adverse events were reported.ConclusiontVNS is a relatively safe adjunct treatment for PD. It has small to moderate therapeutic effects on motor functions and may negatively impact on a few other outcomes. Quality level of the evidence is low and further research is warranted.Systematic review registrationhttps://www.crd.york.ac.uk/prospero/#recordDetails, identifier CRD42024503322 (PROSPERO).
Berberine alleviates enterotoxigenic Escherichia coli-induced intestinal mucosal barrier function damage in a piglet model by modulation of the intestinal microbiome
Authors: Du Min , Liu Xinran , Ji Xu , Wang Yue , Liu Xiaodan , Zhao Chunfang , Jin Erhui , Gu Youfang , Wang Hongyu , Zhang Feng 
Published: January 2025

IntroductionEnterotoxic Escherichia coli (ETEC) is the main pathogen that causes diarrhea, especially in young children. This disease can lead to substantial morbidity and mortality and is a major global health concern. Managing ETEC infections is challenging owing to the increasing prevalence of antibiotic resistance. Berberine, categorized as a substance with similarities in “medicine and food,” has been used in China for hundreds of years to treat gastrointestinal disorders and bacteria-induced diarrhea. This study investigated the preventive effect of dietary berberine on the intestinal mucosal barrier induced by ETEC and the microbial community within the intestines of weaned piglets.MethodsTwenty-four piglets were randomly divided into four groups. Piglets were administered either a standard diet or a standard diet supplemented with berberine at concentrations of 0.05 and 0.1%. and orally administered ETEC or saline.ResultsDietary supplementation with berberine reduced diamine oxidase, d-lactate, and endotoxin levels in piglets infected with ETEC (P &lt; 0.05). Berberine increased jejunal villus height, villus/crypt ratio, mucosal thickness (P &lt; 0.05), and goblet cell numbers in the villi and crypts (P &lt; 0.05). Furthermore, berberine increased the optical density of mucin 2 and the mucin 2, P-glycoprotein, and CYP3A4 mRNA expression levels (P &lt; 0.05). Berberine increased the expressions of zonula occludins-1 (ZO-1), zonula occludins-2 (ZO-2), Claudin-1, Occludin, and E-cadherin in the ileum (P &lt; 0.05). Moreover, berberine increased the expression of BCL2, reduced intestinal epithelial cell apoptosis (P &lt; 0.05) and decreased the expression of BAX and BAK in the duodenum and jejunum, as well as that of CASP3 and CASP9 in the duodenum and ileum (P &lt; 0.05). Berberine decreased the expression of IL-1β, IL-6, IL-8, TNF-α, and IFN-γ (P &lt; 0.05) and elevated total volatile fatty acids, acetic acid, propionic acid, valeric acid, and isovaleric acid concentrations (P &lt; 0.05). Notably, berberine enhanced the abundance of beneficial bacteria including Enterococcus, Holdemanella, Weissella, Pediococcus, Muribaculum, Colidextribacter, Agathobacter, Roseburia, Clostridium, Fusicatenibacter, and Bifidobacterium. Simultaneously, the relative abundance of harmful and pathogenic bacteria, such as Prevotella, Paraprevotella, Corynebacterium, Catenisphaera, Streptococcus, Enterobacter, and Collinsella, decreased (P &lt; 0.05).DiscussionBerberine alleviated ETEC-induced intestinal mucosal barrier damage in weaned piglets models. This is associated with enhancement of the physical, chemical, and immune barrier functions of piglets by enhancing intestinal microbiota homeostasis.