bluuebunny/crossref_metadata_2025_split huggingface dataset:
Novel endovascular transmural technique for pharmacological block of superior cervical ganglion prevents sympathetic-mediated cerebral vasospasm
Authors: Kim Wi Jin , Samarage Hasitha Milan , Jafari Matiar , Zarrin David , Goel Keshav , Qi Xin , Wang Anthony C , Johnson Jeremiah , Colby Geoffrey P 
Published: July 2023

BackgroundSympathetic-mediated vasoconstriction from the superior cervical ganglion (SCG) is a significant contributor to cerebral vasospasm. Inhibition of the SCG has been shown to improve cerebral blood flow and reverse cerebral vasospasm in swine models. We evaluated the efficacy of a novel minimally invasive endovascular approach to target and pharmacologically inhibit the SCG, using a Micro-Infusion Device for transmural drug delivery.MethodsEight SCGs in four Yorkshire swine were surgically identified. After confirming appropriate sympathetic-mediated intracranial vasoconstriction response with SCG stimulation, an endovascular Micro-Infusion Device was used for transmural targeting of the SCG and delivery of 1.5–2 mL of 1% lidocaine-contrast mixture to the perivascular space. Digital subtraction angiography was obtained at: (1) baseline; (2) with SCG stimulation; and (3) after lidocaine delivery to the SCG using the Micro-Infusion Device with concurrent SCG stimulation. Vessel diameters were measured and compared.ResultsEndovascular transmural delivery of lidocaine to the SCG and carotid perivascular tissue using the Micro-Infusion Device successfully inhibited sympathetic-mediated vasoconstriction response. Measured vessel diameters after lidocaine delivery were comparable to baseline despite SCG stimulation.ConclusionA novel endovascular technique of transmural delivery of lidocaine to the SCG and carotid artery perivascular tissues successfully inhibits the sympathetic input to the cerebral vasculature and modulates sympathetic-mediated cerebral vasospasm. These results suggest promising steps towards translation to potential clinical use for patients suffering from cerebral vasospasm.
Gaussian quantum steering in a nondegenerate three-level laser
Authors: Boukhris B. , Tirbiyine A. , El Qars J. 
Published: July 2023

Steering is a type of nonseparable quantum correlation, where its inherent asymmetric feature makes it distinct from Bell nonlocality and entanglement. In this paper, we investigate quantum steering in a two-mode Gaussian state [Formula: see text] coupled to a two-mode vacuum reservoir. The mode [Formula: see text] ([Formula: see text]) is emitted during the first (second) transition of a nondegenerate three-level cascade laser. By means of the master equation of the state [Formula: see text], we derive the analytical expression of the steady-state covariance matrix of the modes [Formula: see text] and [Formula: see text]. Using realistic experimental parameters, we show that the state [Formula: see text] can exhibit asymmetric steering. Furthermore, by an appropriate choice of the physical parameters of the state [Formula: see text], we show that one-way steering can be achieved. Essentially, we demonstrate that one-way steering can, in general, occur only from [Formula: see text]. Besides, we perform a comparative study between the steering of the two laser modes and their Gaussian Rényi-2 entanglement. As a result, we found that the entanglement and steering behave similarly in the same circumstances, i.e. both of them decay under dissipation effect; moreover, they can be well enhanced by inducing more and more quantum coherence in the state [Formula: see text]. In particular, we found that the steering remains always less than the Gaussian Rényi-2 entanglement.
Left Ventricular Gene Expression in Heart Failure With Preserved Ejection Fraction—Profibrotic and Proinflammatory Pathways and Genes
Authors: Ye Bo , Bradshaw Amy D. , Abrahante Juan E. , Dragon Julie A. , Häußler Tim N. , Bell Stephen P. , Hirashima Fuyuki , LeWinter Martin , Zile Michael R. , Meyer Markus 
Published: July 2023

BACKGROUND:
Heart failure with preserved ejection fraction (HFpEF) is increasingly prevalent and has few treatments. The molecular mechanisms and resultant signaling pathways that underlie the development of HFpEF are poorly defined. It has been proposed that activation of proinflammatory pathways plays a role in the development of cardiac fibrosis. The signature of gene expression (transcriptome) of previously validated left ventricular biopsies obtained from patients with HFpEF and matched referent controls allows for an unbiased assessment of proinflammatory and profibrotic signaling pathways and genes.

METHODS:
Epicardial left ventricular biopsies from stringently selected HFpEF patients (HFpEF, n=16) and referent control patients (CTR, n=14) were obtained during aortocoronary bypass surgery. The subepicardial myocardium was flash-frozen to build a repository that was parallel-processed for RNA sequencing to allow for an unsupervised in-depth comparison of the left ventricular transcriptome.

RESULTS:

The average patient age was 67±10 years. When compared with controls, patients with HFpEF were hypertensive with a higher body mass index (kg/m
2
: 30±5 versus 37±6;
P
<0.01) and elevated NT-proBNP levels (pg/mL: 155 [89–328] versus 1554 [888–2178];
P
<0.001). The transcriptome analysis revealed differential expression of 477 genes many of which were involved in profibrotic pathways including extracellular matrix production and posttranslational modification but no proinflammatory signature.

CONCLUSIONS:
The transcriptome analysis of left ventricular myocardial samples from patients with HFpEF confirms an overabundant extracellular matrix gene expression, the basis of myocardial fibrosis, without a signature of activated proinflammatory pathways or genes.
A New Year-Round Weather Regime Classification for North America
Authors: Lee Simon H. , Tippett Michael K. , Polvani Lorenzo M. 
Published: July 2023

Abstract
Weather regimes defined through cluster analysis concisely categorize the anomalous regional circulation pattern on any given day. Owing to their persistence and low dimensionality, regimes are increasingly used in subseasonal-to-seasonal prediction and in analysis of climate variability and change. However, a limitation of existing regime classifications for North America is their seasonal dependence, with most existing studies defining regimes for winter only. Here, we normalize the seasonal cycle in daily geopotential height variance and use empirical orthogonal function analysis combined with k-means clustering to define a new set of year-round North American weather regimes: the Pacific Trough, Pacific Ridge, Alaskan Ridge, and Greenland High regimes. We additionally define a “No Regime” state to represent conditions close to climatology. To demonstrate the robustness of the classification, a thorough assessment of the sensitivity of the clustering solution to various methodological choices is provided. The median persistence of all four regimes, obtained without imposing a persistence criterion, is found to be one week, approximately 3 times longer than the median persistence of the No Regime state. Regime-associated temperature and precipitation anomalies are reported, together with the relationship between the regimes and modes of climate variability. We also quantify historical trends in the frequency of the regimes since 1979, finding a decrease in the annual frequency of the Pacific Trough regime and an increase in the summertime frequency of the Greenland High regime. This study serves as a foundation for the future use of these regimes in a variety of weather and climate applications.

Significance Statement
Weather regimes provide a simple way of classifying daily large-scale regional weather patterns into a few predefined types. Existing methods usually define regimes for a specific season (typically winter), which limits their use, or provides only a minimal assessment of their robustness. In this study, we objectively quantify four weather regimes for use year-round over North America, while we classify near-normal conditions as No Regime. The four regimes represent persistent large-scale weather types that last for about a week and occasionally much longer. Our new classification can be applied to subseasonal-to-seasonal forecasts and climate model output to diagnose recurrent weather types across the North American continent.
The Obesity Paradox in Thyroid Surgery: Is Higher BMI Protective Against Hypoparathyroidism?
Authors: Fields Tyler , Ramonell Kimberly , Fazendin Jessica , Gillis Andrea , Buczek Erin , Porterfield John , Chen Herbert , Lindeman Brenessa 
Published: July 2023

Background With a demonstrated association between adiposity and parathyroid hormone (PTH) levels, we hypothesized that patients with a higher body mass index (BMI) would have lower rates of postoperative hypoparathyroidism following total thyroidectomy. Methods retrospective review of patients undergoing total thyroidectomy from 2015 to 2021. Demographics, BMI, surgical indications, and laboratory data including pre- and postoperative PTH values were examined. Results Of the 352 patients with complete clinicopathologic data, most were female (n = 272, 77.3%) with an average age of 42.7 (SD+/−19.4). Obese (BMI 30-39.99) was most common BMI group (n = 108, 30.8%), with 11.7% (n = 41) morbidly obese (BMI > 40). Morbidly obese patients had significantly higher postoperative PTH levels than BMI < 18.5 (46.0 vs 19.3 pg/mL, P = .004). Patient race was significantly associated with pre- and postoperative PTH ( P = .03, P = .004.) On multivariable analysis, preoperative PTH, race, and BMI were independent predictors of higher postoperative PTH ( P < .05 for all). Discussion Patients with higher BMI and non-white race have relative protection from postoperative hypoparathyroidism.
Resistant distal femoral nonunion treated with combined nail/plate construct and reamer-irrigator-aspirator technique
Authors: Zhang Jin-Hui , Liu Hui , Cai Tao-Yi , Lin Yong-Zhi , Wu Jin 
Published: July 2023

Objective This study was performed to evaluate the effectiveness of intramedullary nailing and a lateral locking plate combined with the reamer-irrigator-aspirator (RIA) bone grafting technique for resistant distal femoral nonunion. Methods This retrospective observational study was performed from January 2018 to December 2021 and involved five patients who presented with resistant distal femoral nonunion despite undergoing several surgeries. They were treated with intramedullary nailing and a lateral locking plate combined with the RIA bone grafting technique. Postoperative follow-up was performed to observe the healing time, and functional outcomes were evaluated using the Lower Extremity Functional Scale (LEFS). Results After the patients had been monitored for a mean of 17.9 months, complete bone healing was observed in every patient (mean healing time of 4.8 months). Postoperative wound failure in an older patient was successfully treated with resuturing and nutritional assistance. At the last follow-up, the mean LEFS score was 71.2/80 and the mean knee flexion was 109 degrees. Conclusions Our study demonstrates that combining intramedullary nailing and a lateral locking plate with the RIA bone grafting technique enhances biological properties, provides good structural support, and achieves good union and functional results in the management of resistant nonunion of the distal femur.
Hemodynamic and Management Perspectives in Heart Failure Patients
Authors: Parvathareddy Krishna Mala Konda Reddy , Nagula Praveen 
Published: July 2023

Introduction Prevalence of heart failure (HF) is increasing, and right ventricular (RV) dysfunction is an independent predictor of mortality. Accurate echocardiographic evaluation of RV function is challenging and novel parameters which can aid in prediction RV function are required. Pulmonary artery Pulsatility index (PAPi) is one such parameter. Patients and Methods We conducted a prospective study in 33 patients admitted with symptoms of HF and severe LV dysfunction (EF < 35) who underwent right heart catheterization and followed them for 6 months. Primary objective was to determine the association of PAPi with hospitalizations and mortality in short-term follow-up in HF patients. Clinical, demographic, echocardiographic, and catheterization data were recorded. PAPi was calculated as a ratio of pulmonary artery pulse pressure and right atrial pressure. Results The mean age of population was 47 years (±12). Males were 20(61%) and females were 13(39%). Median PAPi in the study population was 2.42 (IQR-1.83). The study population were stratified into 3 groups based on 10th, 50th, and 90th percentile PAPi and on comparisons between groups PAPi was significantly associated with hospitalization at 1 and 6 months in univariate ( P = .03 and P = .03 respectively) and in multivariate analysis ( P = .04). PAPi was not found to be associated with mortality at 1 month or 6 months. However, the absolute mortality was low in the study population (n = 4). Conclusion PAPi has been found to be a strong and an independent predictor of hospitalization at 1 month and 6 months. Comments Advanced HF is seen in 1 out of 5 patients with HF. It is characterized by refractory symptoms, multiple hospitalizations, fluid retention, elevated natriuretic peptides, and decreased cardiac output often requiring inotropes. The survival rate is poor. The presence of right ventricular dysfunction is an independent predictor of mortality in these patients. Hemodynamic assessment is of importance in these patients to guide regarding further management, ie, use of inotropes, left ventricular assist devices, etc. The PAPi is a marker derived from the formula (PASP-PADP/RAP). 1 The outcome worsens as the PAPi decreases. However, the stroke volume, pulmonary artery capacitance (PAC), and right atrial pressure all have an impact on PAPi. Patients with high PAC will have better PAPi compared to those with low PAC with the stroke volume being constant. 2 Different subsets of HF population have varying PAPI thresholds, with isolated RV infarction having the lowest (0.9) and severe HF having the highest (3.2). 2 Future research should examine the impact of exercise, inotropes, and vasodilators.
The Accuracy of Various Lung Ultrasonography Findings in Predicting the Necessity for Surfactant Treatment in Neonates With Respiratory Distress Syndrome
Authors: Ghadiri Asli Seyed Mohammadsadegh , khalilzadeh Sabetmasouleh Ameneh , Akhoundi Neda , Siami Alireza 
Published: July 2023

Objective: The study was conducted to determine the value of various lung ultrasonography (LUS) findings in diagnosing neonatal respiratory distress syndrome (RDS) and to assess the ability of ultrasonography to predict the need for surfactant treatment. Materials and Methods: This cohort study was conducted on 164 neonates with RDS with a gestational age of over 28 weeks. Transthoracic sonography was performed in all patients in the infant isolet immediately after admission and stabilization of the infant and during the first 6 hours before surfactant treatment. Immediately after the sonogram, the neonates underwent an anterior–posterior view chest radiograph, before and after surfactant treatment. Results: In comparison with clinical manifestations and radiographic findings as the references, the sensitivity and specificity of each of the LUS-related parameters for detecting RDS were as follows: Faded A-lines (95.4% and 76.7%), abnormal B-lines (91.7% and 71.6%), the presence of consolidation (90.9% and 88.3%), the presence of pleural effusion (95% and 86.7%), and alveolar–interstitial syndrome (AIS) (98% and 96%). Conclusion: LUS findings were valuable in assessing and grading the severity of neonatal RDS alongside clinical evaluation. LUS may assist in selecting infants for surfactant therapy and monitoring treatment response.
Genetically encoded phosphatidylserine biosensor for in vitro, ex vivo and in vivo labelling
Authors: Dirvelyte Eimina , Bujanauskiene Daina , Jankaityte Evelina , Daugelaviciene Neringa , Kisieliute Ugne , Nagula Igor , Budvytyte Rima , Neniskyte Urte 
Published: July 2023

Abstract
Background
The dynamics of phosphatidylserine in the plasma membrane is a tightly regulated feature of eukaryotic cells. Phosphatidylserine (PS) is found preferentially in the inner leaflet of the plasma membrane. Disruption of this asymmetry leads to the exposure of phosphatidylserine on the cell surface and is associated with cell death, synaptic pruning, blood clotting and other cellular processes. Due to the role of phosphatidylserine in widespread cellular functions, an efficient phosphatidylserine probe is needed to study them. Currently, a few different phosphatidylserine labelling tools are available; however, these labels have unfavourable signal-to-noise ratios and are difficult to use in tissues due to limited permeability. Their application in living tissue requires injection procedures that damage the tissue and release damage-associated molecular patterns, which in turn stimulates phosphatidylserine exposure.

Methods
For this reason, we developed a novel genetically encoded phosphatidylserine probe based on the C2 domain of the lactadherin (MFG-E8) protein, suitable for labelling exposed phosphatidylserine in various research models. We tested the C2 probe specificity to phosphatidylserine on hybrid bilayer lipid membranes by observing surface plasmon resonance angle shift. Then, we analysed purified fused C2 proteins on different cell culture lines or engineered AAVs encoding C2 probes on tissue cultures after apoptosis induction. For in vivo experiments, neurotropic AAVs were intravenously injected into perinatal mice, and after 2 weeks, brain slices were collected to observe C2-SNAP expression.

Results
The biophysical analysis revealed the high specificity of the C2 probe for phosphatidylserine. The fused recombinant C2 proteins were suitable for labelling phosphatidylserine on the surface of apoptotic cells in various cell lines. We engineered AAVs and validated them in organotypic brain tissue cultures for non-invasive delivery of the genetically encoded C2 probe and showed that these probes were expressed in the brain in vivo after intravenous AAV delivery to mice.

Conclusions
We have demonstrated that the developed genetically encoded PS biosensor can be utilised in a variety of assays as a two-component system of C2 and C2m2 fusion proteins. This system allows for precise quantification and PS visualisation at directly specified threshold levels, enabling the evaluation of PS exposure in both physiological and cell death processes.
Lineage-specific amplification and epigenetic regulation of LTR-retrotransposons contribute to the structure, evolution, and function of Fabaceae species
Authors: Yang Long-Long , Zhang Xin-Yu , Wang Li-Ying , Li Yan-Ge , Li Xiao-Ting , Yang Yi , Su Qing , Chen Ning , Zhang Yu-Lan , Li Ning , Deng Chuan-Liang , Li Shu-Fen , Gao Wu-Jun 
Published: July 2023

Abstract
Background
Long terminal repeat (LTR)-retrotransposons (LTR-RTs) are ubiquitous and make up the majority of nearly all sequenced plant genomes, whereas their pivotal roles in genome evolution, gene expression regulation as well as their epigenetic regulation are still not well understood, especially in a large number of closely related species.

Results
Here, we analyzed the abundance and dynamic evolution of LTR-RTs in 54 species from an economically and agronomically important family, Fabaceae, and also selected two representative species for further analysis in expression of associated genes, transcriptional activity and DNA methylation patterns of LTR-RTs. Annotation results revealed highly varied proportions of LTR-RTs in these genomes (5.1%~68.4%) and their correlation with genome size was highly positive, and they were significantly contributed to the variance in genome size through species-specific unique amplifications. Almost all of the intact LTR-RTs were inserted into the genomes 4 Mya (million years ago), and more than 50% of them were inserted in the last 0.5 million years, suggesting that recent amplifications of LTR-RTs were an important force driving genome evolution. In addition, expression levels of genes with intronic, promoter, and downstream LTR-RT insertions of Glycine max and Vigna radiata, two agronomically important crops in Fabaceae, showed that the LTR-RTs located in promoter or downstream regions suppressed associated gene expression. However, the LTR-RTs within introns promoted gene expression or had no contribution to gene expression. Additionally, shorter and younger LTR-RTs maintained higher mobility and transpositional potential. Compared with the transcriptionally silent LTR-RTs, the active elements showed significantly lower DNA methylation levels in all three contexts. The distributions of transcriptionally active and silent LTR-RT methylation varied across different lineages due to the position of LTR-RTs located or potentially epigenetic regulation.

Conclusion
Lineage-specific amplification patterns were observed and higher methylation level may repress the activity of LTR-RTs, further influence evolution in Fabaceae species. This study offers valuable clues into the evolution, function, transcriptional activity and epigenetic regulation of LTR-RTs in Fabaceae genomes.
Mobile care - a possible future for emergency care in Sweden
Authors: Teske Christofer , Mourad Ghassan , Milovanovic Micha 
Published: July 2023

Abstract
Introduction
Provision of mobile care at the home of patients appears to become necessary as the population becomes increasingly older. But there are challenges in moving emergency care from hospitals to the home of patients. The aim of the study was therefore to describe the experiences of the mobile care in Sweden.

Method
Semi structured interviews were conducted with 12 persons with experience of mobile care in Sweden, such as nurses, physicians, civil servants and politicians. Qualitative latent content analysis was used as an analysis method.

Result
The results show that cooperation is of utmost importance to achieve functioning mobile care. Cooperation both on an inter-organizational level and on a close team-work is required for all of the involved parties in mobile care to take on a joint responsibility for the patient. As mobile care is primarily provided to elderly multimorbid patients, a comprehensive view on patient care is required in which the patient and their relatives experience security.

Conclusion
Mobile care is seen as a moving care that comes to the seeking person and not the other way around. The resources are distributed where they make the most use, that is, closest to the individual. Mobile care is seen as a complement to the traditional hospital care. This means a different way of working that requires close collaboration between different categories of personnel and organizations, where there should not be any discussions about boundaries, rather, the discussion should include patient’s needs and situation instead.