bluuebunny/crossref_metadata_2025_split huggingface dataset:
Comprehensive Analysis of lncRNA and mRNA Expression Profile of
Macrophage RAW264.7 Stimulated by Antimicrobial Peptide BSN-37
Authors: Qin Ting , Liu Mingcheng , Lv Yanhe , Zheng Airong , Wang Lei , Wu Yundi , Kasianenko Oksana , Wei Xiaobing , Teng Zhanwei , Xia Xiaojing , Hu Jianhe 
Published: August 2023

Background:
BSN-37, a novel antimicrobial peptide (AMP) containing 37 amino acid
residues isolated from the bovine spleen, has not only antibacterial activity but also immunomodulatory
activity. Recent evidence shows that long non-coding RNAs (lncRNAs) play an important
role in regulating the activation and function of immune cells. The purpose of this experiment was
to investigate the lncRNA and mRNA expression profile of mouse macrophages RAW264.7 stimulated
by bovine antimicrobial peptide BSN-37.

Methods:
The whole gene expression microarray was used to detect the differentially expressed lncRNA
and mRNA between antimicrobial peptide BSN-37 activated RAW264.7 cells and normal
RAW264.7 cells. KEGG pathway analysis and GO function annotation analysis of differentially expressed
lncRNAs and mRNA were carried out. Eight kinds of lncRNAs and nine kinds of mRNA
with large differences were selected for qRT-PCR verification, respectively.

Results:
In the current study, we found that 1294 lncRNAs and 260 mRNAs were differentially expressed
between antibacterial peptide BSN-37 treatment and control groups. Among them,
Bcl2l12, Rab44, C1s, Cd101 and other genes were associated with immune responses and were all
significantly up-regulated. Mest and Prkcz are related to cell growth, and other genes are related to
glucose metabolism and lipid metabolism. In addition, some immune-related terms were also
found in the GO and KEGG analyses. At the same time, real-time quantitative PCR was used to
verify selected lncRNA and mRNA with differential expression. The results of qRT-PCR verification
were consistent with the sequencing results, indicating that our data were reliable.

Conclusion:
This study provides the lncRNA and mRNA expression profiles of RAW264.7
macrophages stimulated by antimicrobial peptide BSN-37 and helps to provide a reference value
for subsequent studies on lncRNA regulation of antimicrobial peptide BSN-37 immune function.
LncRNA LINC00847 Accelerates Melanoma Progression by Regulating
MiR-133a-3p/TGFBR1 Axis
Authors: Jiang Lei , Shi Xiufang , Liu Yanxin , Chen Huaxia 
Published: August 2023

Aims:
Growing evidence has suggested that lncRNAs play a regulatory role in tumorigenesis.
Dysregulation of a newly identified lncRNA (LINC00847) has been involved in several
tumors. Nevertheless, the expression and roles of lncRNAs in skin melanoma remain unclear.
Therefore, we attempted to investigate the expressions and roles of lncRNAs in this study.

Materials and Methods:
Expression levels of LINC00847 were quantified in tissue samples from
the TCGA database and clinically recruited participants. LINC00847 was inhibited in cells by
transfecting with si-LINC00847 or si-NC. Expressions of LINC00847 and miR-133a-3p were determined
using RT-qPCR, and the TGFBR1 level was determined using Western blotting. Targeting
sites of LINC00847 with miR-133a-3p and miR-133a-3p with TGFBR1 were predicted by bioinformatic
tools and proved by dual-luciferase reporter system and RNA immunoprecipitation. Cell
proliferation, invasion, and migration abilities were assessed using CCK8, cell colony formation,
cell wound scratch, and transwell assay, respectively.

Results:
In both TCGA and clinical cohorts, the expression of LINC00847 was abnormally upregulated
in skin melanoma tissues than that of benign nevus. Besides, LINC00847 expression increased
more markedly in A375 and SK-MEL-28 cells than in normal epidermal melanocytes
(HEMa-LP cells). LINC00847 knockdown remarkably restrained skin melanoma cell proliferation,
metastasis, and wound healing rate. Furthermore, miR-133a-3p/TGFBR1 was the downstream target
for LINC00847. LINC00847 negatively regulated miR-133a-3p expression in skin melanoma
cells. Both miR-133a-3p inhibitors and TGFBR1 vector transfection reversed the effect of
LINC00847 silence in skin melanoma cells.

Conclusion:
LINC00847 was highly expressed in skin melanoma, and its overexpression accelerated
the malignant tumor behavior of skin melanoma cells. The miR-133a-3p /TGFBR1 axis was
involved in the roles of LINC00847 in skin melanoma.
Prognostic Role of Unfolded Protein Response-Related Genes in Hepatocellular
Carcinoma
Authors: Zhang Shuqiao , Li Xinyu , Zheng Yilu , Hu Hao , Liu Jiahui , Zhang Shijun , Tang Chunzhi , Mo Zhuomao , Kuang Weihong 
Published: August 2023

Aims:
To reveal the prognostic role of unfolded protein response (UPR) -related genes
in hepatocellular carcinoma (HCC).

Background:
Hepatocellular carcinoma is a genetically heterogeneous tumor, and the prediction of
its prognosis remains a challenge. Studies elucidating the molecular mechanisms of UPR have
rapidly increased. However, the UPR molecular subtype characteristics of the related genes in
HCC progression have yet to be thoroughly studied.

Objective:
Conducting a comprehensive assessment of the prognostic signature of genes related to
the UPR in patients with HCC can advance our understanding of the cellular processes contributing
to the progression of HCC and offer innovative strategies in precise therapy.

Methods:
Based on the gene expression profiles associated with UPR in HCC, we explored the
molecular subtypes mediated by UPR-related genes and constructed a UPR-related genes signature
that could precisely predict the prognosis for HCC.

Results:
Using microarray data of HCC patients, differentially expressed UPR-related genes
(DEGs) were discovered in malignancies and normal tissues. The HCC was classified into two
molecular subtypes by the NMF algorithm based on DEGs modification of the UPR. Moreover, we
developed a UPR-related model for predicting HCC patients' prognosis. The robustness of the UPR-
related model was confirmed in external validation. Moreover, we analyzed immune responses
in different risk groups. Analysis of immune functions revealed that Treg, Macrophages, aDCs,
and MHC class-I were significantly up-regulated in high-risk HCC. At the same time, cytolytic activity
and type I and II INF response were higher in a low-risk subgroup.

Conclusion:
This study identified two UPR molecular subtypes of HCC and developed a ten-gene
HCC prognostic signature model (EXTL3, PPP2R5B, ZBTB17, CCT3, CCT4, CCT5, GRPEL2,
HSP90AA1, PDRG1, and STC2), which can robustly forecast the progression of HCC.
Role of Magnetic Resonance Imaging in the Diagnosis of Placenta Accreta
Authors: Niu Lei , Cui Wen , Zhu Chunxia , Lu Xiaoning , Wang Yongkang , Wang Feng 
Published: August 2023

Introduction:
To analyze the value of magnetic resonance imaging (MRI) in suspicious cases for prenatal detection of placenta accreta (PA).

Materials and Methods:
A total of 50 placental MRI exams performed on a 1.5T scanner were retrospectively reviewed by two radiologists in consensus.HASTE(half-Fourier acquisition single-shot turbo spin echo)and True-FISP (true fast imaging with steady-state precession) sequences were acquired. Findings from MRI were compared with the final diagnosis, which was determined by clinical findings at delivery and pathological examination of specimens.

Results:
Of 50 pregnant women in the analysis, 33 required cesarean hysterectomy, and 17 underwent cesarean delivery.MRI signs such as myometrial
thinning, loss of T2 hypointense interface(loss of retroplacental clear space on US), heterogenous intraplacental sign, and intraplacental T2 dark
bands were more likely to be seen in this group.

Conclusion:
MRI is particularly useful in cases where US is inconclusive and to assess the extent to which the placenta penetrates the uterine serosa and invades outward into surrounding tissues.MRI has become a routine examination for patients with suspected PA in clinical practice.

:
In this group, the cases that were finally clinically and pathologically confirmed were 12, 16, and 22 cases of placenta accreta vera, placenta
increta, and placenta percreta respectively.
Insight into JNK Inhibition-based Strategy for the Treatment of
Alzheimer's Disease
Authors: Zyuz’kov Gleb Nikolaevich , Miroshnichenko Larisa Arkad`evna , Polykova Tatyana Yur`evna , Simanina Elena Vladislavovna , Chayikovskyi Alexander Vasil`evich , Kotlovskaya Larisa Yur`evna 
Published: August 2023

Background:
Existing drugs for the treatment of Alzheimer's disease do not meet the basic
requirements. Therefore, it is necessary to search for fundamentally new targets for the discovery of
drugs for Alzheimer's disease (AD). When creating approaches to stimulate neurogenesis, such a search
is promising to conduct as part of the development of a strategy for targeted regulation of intracellular
signal transduction in regeneration-competent cells (RCCs). Moreover, the participation of c-Jun Nterminal
kinases (JNK) in the regulation of the functions of the nervous tissue and neuroglial cells is
known.

Objective:
The aim of the work was to study the effect of the JNK inhibitor (IQ-1S) on exploratory behavior
and cognitive functions in aged mice (16-month-old male C57BL/6 mice as a model of AD) in
combination with the dynamics of shifts in the RCCs functioning (nervous tissue progenitors: neural
stem cells (NSCs) and neuronal-committed progenitors (NCPs); neuroglial cells: astrocytes, oligodendrocytes,
microglial cells).

Methods:
The studies were carried out on male C57BL/6 mice aged 16 months as model of Alzheimer's
disease. We studied the effect of the JNK inhibitor on exploratory behavior, conditioned reflex activity,
and on the functioning of RCCs in the subventricular zone of the cerebral hemispheres (SVZ). NCPs
and neuroglial cells of different types were obtained by immunomagnetic separation.

Results:
We observed significant changes in exploratory behavior and impaired conditioned reflex activity
in aged C57BL/6 mice. The administration of the JNK inhibitor led to a significant correction of
age-related behavioral and memory disorders in aged mice. At the same time, against the background of
JNK inhibition-based therapy, an increase in mitotic activity and the content of both NSCs and NCPs in
the SVZ was noted. However, these shifts were more pronounced in committed precursors. The phenomenon
of the inhibition of NSC specialization under the influence of a pharmacological agent was
also revealed. In addition, the JNK inhibitor caused an increase in the secretion of neurotrophic growth
factors by oligodendrocytes and microglial cells.

Conclusion:
The findings open up prospects for the development of JNK targeting-based approaches
for the treatment of AD.
Investigating the Association of rs4962416 and rs6465657 with Prostate
Adenocarcinoma in the Iranian Population
Authors: Hemmati Tannaz , Angaji Seyed Abdolhamid , Beikzadeh Behnaz , Alibeik Hengameh , Roudi Raheleh , Narouie Behzad 
Published: August 2023

Background:
Prostate cancer is one of the most commonly diagnosed malignancies
in the developed world. Despite other risk factors like age, diet, environment and the
pathogenesis of prostate cancer, recent advances in molecular genetics suggest that genetic
inheritance plays an important role in prostate cancer.

Objective:
We attempted to analyze the association of SNPs rs4962416 and rs6465657 in
the development of prostate cancer. A better understanding of the association of SNPs in
prostate cancer susceptibility may improve risk prediction, improve precision mapping, and
provide new insights into the underlying pathophysiology of prostate cancer. To date, no
one has investigated these two SNPs in the Iranian populations, and according to the
heterogeneity that exists, SNPs in communities should be examined separately.

Methods:
This case-control study includes 82 people with prostate adenocarcinoma as cases
and 96 people with benign prostatic hyperplasia (BPH) as controls. Genotyping of each participant
was done by TETRA ARMS-PCR method and for statistical analysis chi-squared,
Fisher’s exact logistic regression was used to find the SNPs associated with prostate cancer.

Result:
The frequency of the polymorphisms rs4962416 and rs6465657 in the prostate adenocarcinoma
group was evaluated compared to the BPH control group (p-value < 0.05%) to
choose the meaningful SNP. For rs4962416, we didn’t find any meaningful association with
prostatic cancer (p=0.402) but for rs6465657 there was a significant difference between
genotype frequency (p=0.001).

Conclusion:
rs6465657 polymorphism which is associated with prostate cancer, can be chosen
as a biomarker for this cancer and there should be more investigation on this SNP as
these results need to be confirmed in a larger population.
Taste Masking Cetirizine Hydrochloride using Ion-exchange Resins
Authors: Sridhar Vanga , Thirupathi Kaluvala , Sridhar Mittapally 
Published: August 2023

Aim:
The scope of the study is to determine the exact drug content present in the dry resinate and the utilization of ion-exchange resin in taste masking highly bitter cetirizine hydrochloride (CTZ). The Amberlite® IRP-69 cross-linked polystyrene was used.

Objective:
The objectives of this study are drug loading, drug–resin ratios, effect of particle size determination, and qualitative evaluation of taste masking ion-exchange resins.

Background:
The resinate (drug–resin complex) preparation process was completed by batch method in the ratio of 1:1, 1:2, and 1:3. The loaded drug amount in the resin was evaluated spectrophotometrically by measuring the remaining drug to be loaded in the solution at 278nm. Different sieve sizes of Amberlite® IRP-69 resins were employed to determine the effect of the particle size in drug loading and drug releasing profiles in simulated gastric fluid without enzymes. The X-ray diffractograms of cetirizine hydrochloride, Amberlite® IRP-69, and a mixture of cetirizine hydrochloride with resins (resinate) were obtained by using an X-ray diffractometer.

Results:
Resinates were developed by using single-batch and double-batch methods. Both of them are giving almost the same results of drug loading into ion-exchange resins. We selected 1:1 drug–resin ratio to see the effect of the particle size on the rate and extent of drug loading and drug releasing from the complex. In the 1:1 drug-to-resin ratio, almost 78% of the drug was involved in the complex after 24 hours; in the case of 2:1 and 3:1 ratios, only 49% and 25% of the drug were involved in the complex, respectively.

Conclusion:
Among many taste masking methods, the formation of resinates with an ion-exchange resin is simple and inexpensive. The present work reveals that the degree of bitterness of cetirizine hydrochloride is reduced to zero by strong cation-exchange resin. The present study deals on the scope of utilizing ion-exchange resins in taste masking cetirizine hydrochloride (CTZ), a highly bitter drug.
Introducing a New Method for DPMU in Detecting the Type and Location
of the Fault
Authors: Zand Mohammad , Nasab Morteza Azimi , Padmanaban Sanjeevikumar , Khan Bassam 
Published: August 2023

Introduction:
Nowadays, due to the increasing development of distribution networks, their
safety and high-reliability performance are of particular importance. One of the most important problems
that endanger the security and reliability of these networks is the creation of some faults in them.
In case of a fault in the network, identifying its location and type can be of great help in repairing faulty
equipment. Also, by detecting the disconnection of one of the equipment or lines, it is possible to prevent
accidents in the network.

Methods:
Phasor Measurement Unit (PMU) has been widely and successfully used as Transmission-
Phasor Measurement Unit (T-PMU). The reporting time of PMUs is much shorter than the old Supervisory
Control and Data Acquisition (SCADA) systems. They can provide synchronized phasor measurements
that can generate voltage phasors of different network nodes. This study aimed to investigate
the various applications of phasor measurement units in distribution networks and present a new method
for detecting and analyzing the location and type of fault and topology fault of the distribution network
using the Internet of Things (IOT) analysis method.

Results:
To implement this method, it is necessary to measure different parameters of the distribution
network before and after the occurrence of a fault, which is used by the DPMU for measurement. The
simulation results indicate that for both single-topology and multi-topology faults, the proposed method
has higher accuracy and better detection than the remaining common methods and effectively detects
single-topology and multi-topology faults in the distribution network.

Conclusion:
This method can provide a more accurate network topology to estimate the state of the
distribution network, which improves the accuracy of the state estimation and is suitable for implementing
various advanced functions of the distribution management system.
THE FEKETE -SZEGO ̈ PROBLEM OF ANALYTIC FUNCTIONS BASED ON THE DEFERENtIAL OPERTOR AND CERTAIN SUBCLASSES
Authors: Văn an lý  
Published: August 2023

In this paper I use the operators Dmβ,η(η, φ)f, Sm(d, β, η) and Cm(d, β, η).To establish |a3−μa22 | -functional inequalities for the Fekete-Szeg ̈o problem. That’s my main result.
Effectiveness of Friction Force Reduction in Sliding Motion Depending on the Frequency of Longitudinal Tangential Vibrations, Sliding Velocity and Normal Pressure
Authors: Leus Mariusz , Gutowski Paweł , Rybkiewicz Marta 
Published: August 2023

Abstract
The article presents the results of experimental research and simulation analyses of the influence of slip velocity, normal pressures and vibration frequency on the effectiveness of friction force reduction carried out in sliding motion in the presence of forced tangential vibrations. In experimental studies, changes in the driving force were measured during the slip of the upper body over the vibrating lower body. The direction of these vibrations was parallel both to the contact plane and to the direction of movement of the shifted body. The simulation tests were carried out in the Matlab/Simulink environment through the use of numerical procedures that were specially created for this purpose. Dynamic friction models considering the tangential compliance of contact and the phenomenon of pre-sliding displacement were used for calculations. The paper presents the designated values of the so-called coefficient of average friction force reduction in sliding motion for the following friction pairs: steel C45–steel C45, steel C45–cast iron GGG40 and steel C45–polytetrafluoroethy-lene PTFE (Teflon). The results of numerical analyses were in good agreement with those of experimental tests. A significant dependence of the level of average friction force reduction on the frequency of forced vibrations, sliding velocity as well as the kind of sliding pair material, and normal pressures was shown.
Owls’ responses to forest conservation in the Alps
Authors: Nemček Vladimír , Kohl Barbara 
Published: August 2023

Abstract
As inhabitants of cavities, some owl species benefit from natural processes, different tree compositions and higher volumes of dead wood. We assumed that protected areas would have a positive impact on the owl community. We compared the abundance of calling owls on 22 lines in managed versus protected forests. Here, we recorded the acoustic communication of owls. The composition of the owl community did not differ between areas. However, we found the impact of forest landscape integrity index and altitude on the diversity of owls. Forests in protected areas probably need time to develop natural and heterogeneous habitat structures. The conservation priority should be to increase the integrity of the forests. Our results also confirmed that managed forests can have a high diversity of owls.
PERTARUNGAN KATA DALAM SLOGAN POLITIK (KONTEKS PEMILIHAN KEPALA DAERAH DI MALUKU): SUATU KAJIAN ANALISIS WACANA KRITIS
Authors: Pesiwarissa Leonora F. , Hiariej Chrissanty , Wuarlela Mouren 
Published: August 2023

Studi wacana sebagai bagian dari perkembangan ilmu linguistik yang berorientasi pada lingustik fungsional, membahas tentang bahasa dalam penggunaannya dan akan selalu terkait konteks. Melalui wacana, seseorang atau sekelompok masyarakat dapat diidentifikasi, bahkan ideologi, kepribadian, dan perilakunya dapat dikenali. Pada kenyataannya, wacana sering dimanfaatkan untuk kepentingan kekuasaan, salah satunya wacana politik. Wacana politik tidak terlepas dari stuktur-stuktur lingual yang menyusunnya. Melalui aspek kebahasaan itulah wacana politik dideksripsikan dan dianalisis untuk mengungkap ideologi dan kekuasaan yang tersembunyi dibalik wacana politik tersebut. Untuk tujuan politik dalam konteks pemilihan kepala daerah, slogan dijadikan sebagai salah satu alat yang ampuh untuk menarik perhatian masyarakat hanya dengan ‗kata-kata‘ demi tujuan utama yakni kemenangan. Pemanfaatan slogan memungkinkan adanya pertarungan secara tidak langsung antarcalon pemimpin maupun antarpemilih. Salah satu bentuk pertarungan yang acapkali tidak disadari dan dipahami oleh masyarakat adalah pertarungan kata. Pendekatan yang digunakan dalam penelitian ini adalah pendekatan kualitatif deksriptif dengan jenis penelitian AWK. Metode pengumpulan data yang digunakan adalah observasi dan wawancara. Analisis data penelitian dilakukan dengan menggunakan metode AWK Fairloucgh. Hasil penelitian menunjukkan bahwa bentuk pertarungan kata dalam slogan sebagai bagian dari wacana politik pada ranah pemilihan kepala daerah Maluku merepresentasikan: (1) Ideologi paslon sebagai bentuk pemertahanan kekuasaan yang tampak pada struktur lingual akronim dari nama paslon, kalimat imperatif berisi ―promosi‖ sebagai pendukung dan penegasan. (2) Ideologi paslon sebagai bentuk perebutan kekuasaan yang tampak pada akronim dari nama paslon, katakata formal dan informal dengan simbol-simbol kedaerahan,dan rujukan pribadi. (3) Cara pandang pemilih terhadap paslon terkait sinergi janji dan realisasi yang merepresentasikan sikap, karakter, tindakan, serta kinerja paslon dalam memimpin.
PENGEMBANGAN PEMBELAJARAN KREATIF DAN INOVATIF UNTUK MENUMBUHKEMBANGKAN SIKAP POSITIF BAHASA MAHASISWA JURUSAN TEKNIK SIPIL, POLITEKNIK NEGERI AMBON
Authors: Wuarlela Mouren 
Published: August 2023

Penguasaan, pemilihan, dan penggunaan bahasa di kalangan mahasiswa khususnya pada jurusan Teknik Sipil, Politeknik Negeri Ambon beragam yakni bahasa Indonesia, bahasa daerah, dan bahasa Inggris. Kebergaman bahasa menjadi identitas dan ciri khas masyarakat Indonesia. Akan tetapi keberagaman bahasa tersebut pun dapat berdampak pada sikap bahasa. Faktanya sikap bahasa mahasiswa belum sepenuhnya bersifat positif. Hal ini berdampak pada bergesernya kedudukan dan fungsi Bahasa Indonesia sebagai bahasa negara dan bahasa pengantar pendidikan. Oleh sebab itu dibutuhkan strategi dalam menumbuhkembangkan sikap bahasa mahasiswa. Salah satu strategi yang dapat digunakan dalam membentuk karakter mahasiswa khususnya sikap mahasiswa terhadap bahasa Indonesia adalah melalui pembelajaran yang kreatif dan inovatif.
Melalui tulisan ini, penulis memaparkan rancangan Pembelajaran Student Center Learning (SCL), Penugasan Berbasis Proyek, dan Pembelajaran berbasis Blended-Learning. Produk akhir dari strategi ini dirancangkan berupa “Stiker dan bros berisi slogan bertema Menumbuhkembangkan Rasa Cinta Bahasa Indonesia” yang dapat disebarkan kepada mahasiswa dan masyarakat sebagai wujud rasa cinta mahasiswa terhadap bahasa Indonesia.