bluuebunny/crossref_metadata_2025_split huggingface dataset:
Quantifying the adverse effects of long COVID on individuals’ health after infection: A Propensity Score Matching design study
Authors: Miao Yudong , Ruizhe Ren , Shen Zhanlei , Li Yi , Zhang Wanliang , Bai Junwen , Zhu Dongfang , Zhang Jingbao , Guo Dan , Tarimo Clifford , Liu Rongmei , Zhao Qiuping , Hu Jianping , Zhou Xue , Dong Wenyong 
Published: September 2023

Abstract
This study assessed the prevalence and influencing factors of long COVID, and measured the difference in health status between long COVID and non-long COVID cases. A total of 3165 COVID-19 patients (53.11% female) completed the questionnaire and provided data on socio-demographic characteristics, lifestyles and health status. The total score of lifestyle assignment ranged from 5 to 21 points. Health status was assessed through self-reported, and the score ranged from 0 to 100 points. The criteria of “persistent symptoms or health problems after 4 weeks (28 days) of COVID-19 infection” issued by the US Centers for Disease Control and Prevention was used to assess whether the individuals had long COVID. The answers revealed that 9.73% of patients were identified long COVID. The health status scores of the long COVID cases 74.79 (95% CI: 72.75 ~ 76.82) was lower than that of the non-long COVID cases 81.06 (95% CI: 80.50 ~ 81.61). Never focused on mental decompression was a common risk factor for the health status of long and non-long COVID cases (β=-25.40, 95% CI: -38.82~-11.98; β=-4.86, 95% CI: -7.51~-2.21). The total lifestyle scores were identified as a mediating factor long COVID infection on individuals’ health, with a mediating effect of 0.2249 (95%CI: 0.0774 ~ 0.4096). In conclusion, the proportion of long COVID cases was low, but they were significantly worse off in health. This study proved that healthy lifestyles including mental decompression should be considered as the core strategy of primary prevention when the epidemic of COVID-19 is still at a low level.
Threshold Sampling
Authors: Rass Stefan , Jakobitsch Max-Julian , Haan Stefan , Hiebler Moritz 
Published: September 2023

Abstract
We consider the problem of sampling elements with some desired property from a large set, without testing the property of interest, but with the (probabilistic) assurance to have at least one match among the random sample. Like in ranked set sampling, we consider an infinite population under study, whose properties of interest are too expensive and/or time-consuming to measure. Unlike RSS, we are void of a ranking mechanism, so our sampling is done entirely blind. We show how it is nonetheless doable to assure, with controllably large likelihood, to either have at least one of the interesting elements in a random sample, or, contrarily, sample with the likewise assurance of not having one of the interesting elements in the sample. Our technique utilizes density bounds for distributions and threshold functions from random graph theory.
Anti-CTLA4 treatment reduces lymphedema risk through a systemic expansion of the FOXP3+ Treg population
Authors: Gousopoulos Epameinondas , Wolf Stefan , Madanchi Matiar , Turko Patrick , Hollmen Maija , Tugues Sònia , von Atzigen Julia , Giovanoli Pietro , Dummer Reinhard , Lindenblatt Nicole , Halin Cornelia , Detmar Michael , Levesque Mitchell 
Published: September 2023

Abstract

Secondary lymphedema is a common sequel of oncologic surgery following lymphatic injury and presents a substantial global health burden for which no pharmacological treatment exists. The infiltration of the affected lymphedematous extremities with CD4+T-cells has been shown to influence lymphedema onset. Various approaches towards their manipulation have emerged as a promising strategy in lymphedema therapy. Here, we show that the modulation of CD4+FOXP3+ regulatory T-cells (Tregs) upon anti-CTLA4 treatment can protect against lymphedema development in melanoma patients and a mouse lymphedema model. A retrospective evaluation of a melanoma patients’ registry revealed that anti-CTLA4 reduces lymphedema risk compared to patients receiving only lymphadenectomy. Importantly, lymphedema tissue biopsies from human and mice presented an increased infiltration with CTLA4+ and Treg cells. To assess the underlying mechanism, anti-CTLA4 was used in the murine-tail lymphedema model, leading to a significant edema reduction and improvement of lymphatic function. The effect of the anti-CTLA4 treatment was attributed to the systemic expansion of Tregs, both in the animal model and in melanoma patients. The data from both, the pre-clinical and human studies indicating the protective effect of anti-CTLA4 against lymphedema, coupled with its anti-tumor properties, identify a promising candidate suitable for quick transition in the clinics.
One Sentence Summary: Anti-CTLA4 treatment reduces lymphedema risk in melanoma patients and lymphedema development in a pre-clinical model via Treg cell modulation.
Neuroprotective and memory enhancing effect of Nose to Brain formulation of 6- Hydroxyflavone in icv-STZ-induced Alzheimer's disease mouse model
Authors: Patil Hitesh , Pardeshi Chandrakant , Khule Siddheshwar , Gagarani Manish 
Published: September 2023

Abstract
Alzheimer's disease characterized by memory and cognitive impairments as well as neurobehavioral abnormalities. Streptozotocin (STZ) can induce a condition analogous to alzheimer's disease by promoting the accumulation of plaques and neurofibrillary tangles, resulted in progressive cognitive decline. 6-hydroxyflavone (6HOF) is a flavone that occurs naturally in the foliage of Barleria prionitis, an Acanthaceae plant species native to India. The purpose of this study was to examine the effects of intranasal formulation of 6-Hydroxyflavon (6HOF-NTB) in mice that had been administered STZ (3mg/kg of icv) unilaterally. Neurodegeneration, and oxidative stress were used to evaluate the impact on working memory and neuroprotection. Memory impairment was developed by intracerebrovascular (icv) injections of streptozotocin (3mg/kg). Upon 21-day administration of 6HOF-NTB and Oral administrations of 6-HOF the behavioral parameter was studied using Morris water maze, novel object recognitions test, Y-maze, EPM etc. and the level of acetylcholinesterase and antioxidants was measured in brain homogenate.In a mouse model of a condition like Alzheimer's, demonstrated significant effect than the STZ-treated group. The 6HOF-NTB (1 mg/kg) increased transfer latency in the EPM, spontaneous alternation in the Y-maze, discrimination index, and novelty preference in the NOR. In addition, in the MWM, the number of platform crossings and time spent in the target quadrants increased, indicating an improvement in working spatial memory. In addition, the intranasal formulation inhibited oxidative stress and acetylcholinesterase levels in the brain, thereby reducing neurodegeneration. In the STZ induced memory impairment model in mice, the intranasal formulation of 6 HOF demonstrated desirable effects on cognitive functions and neuroprotection.
Deep Learning for Protein–peptide binding Prediction: Incorporating Sequence, Structural and Language Model Features
Authors: Chandra Abel , Sharma Alok , Dehzangi Iman , Tsunoda Tatsuhiko , Sattar Abdul 
Published: September 2023

Abstract
Protein-peptide interactions play a crucial role in various cellular processes and are implicated in abnormal cellular behaviors leading to diseases such as cancer. Therefore, understanding these interactions is vital for both functional genomics and drug discovery efforts. Despite a significant increase in the availability of protein-peptide complexes, experimental methods for studying these interactions remain laborious, time-consuming, and expensive. Computational methods offer a complementary approach but often fall short in terms of prediction accuracy. To address these challenges, we introduce PepCNN, a deep learning-based prediction model that incorporates structural and sequence-based information from primary protein sequences. By utilizing a combination of half-sphere exposure, position specific scoring matrices, and pre-trained transformer language model, PepCNN outperforms state-of-the-art methods in terms of specificity, precision, and AUC. The PepCNN software and datasets are publicly available at https://github.com/abelavit/PepCNN.git.
Study on eye movement characteristics of basketball players during free throws under different intensities
Authors: Zhao chunzhou , Li Sunnan , Liu Na 
Published: September 2023

Abstract
The present study aimed to investigate the eye movement characteristics of basketball players during free throws at different intensities. This study selected 20 professional female basketball players wore a mobile eye tracker and made free throws at low, moderate and high exercise intensities. Eye movement data were recorded using the Tobii Glasses 3 eye tracker.Three kinds of exercise intensity under the free throws, front, top,bottom,top left, bottom left and bottom right average number of fixation was significantly different, front, bottom, top left and bottom right average fixation duration were significantly different, average pupil dilation was significantly significant.With the increase of exercise intensity, the total average number of fixation and fixation duration gradually increased, the pupil dilation increased, and the average number of fixation and fixation duration of AOI changed.The increase of exercise intensity leads to the distraction of attention, the automatic calculation ability of vision is reduced, and the information search strategy is decreased. The automatic information processing mode is destroyed and the control ability of the cognitive system is reduced, the fixation stability is changed, and the information processing ability is decreased.
Clinical Application of the Modified Neck-Shoulder Technique Based on Membrane Autopsy in Laparoscopic Totally Extraperitoneal Prosthesis for Indirect Inguinal Hernia
Authors: Pan Guofeng , Xu Yanchang , Chen Jian , Pan Guoyan , He Limei , Weng Jianbin , Li Junpeng , Zhang Weihong 
Published: September 2023

Abstract
Objective: To investigate the anatomical basis and clinical application value of the modified neck-shoulder technique based on membrane autopsy in laparoscopic totally extraperitoneal prosthesis (TEP) for tension-free repairs of indirect inguinal hernia.
Methods:In this retrospective cohort study, we analyzed the clinical data of 136 patients with indirect inguinal hernia who underwent laparoscopic TEP for tension-free repairs in Department of gastroenterological surgery Unit 1, the First Hospital of Putian City, Fujian Province from June 2017 to June 2020. The patients were divided into the modified neck-shoulder technique group (68 cases) and the traditional surgery group (68 cases) according to the different surgical methods. The intraoperative and postoperative conditions of the two groups were compared.
Results:Both the modified neck-shoulder technique group and the traditional surgery group completed the herniorrhaphy. Compared with the traditional surgery group, the modified neck-shoulder technique group had a shorter operation time [(37.15±5.320) min vs. (54.04±5.202) min, t=18.472, p<0.001], less intraoperative blood loss [(5.53±1.634) ml vs. (16.21±3.375) ml, t=23.544, p<0.001], lower incidence of intraoperative peritoneal injury [3 cases (4.41%) vs. 9 cases (13.26%), χ²=3.29, p=0.07], lower intraoperative conversion rate [1 case (1.47%) vs. 8 cases (11.76%), χ²=5.83, p=0.016], and lower incidence of postoperative chronic pain [1 case (1.47%) vs. 12 cases (17.65%), χ²=10.291, p=0.001], all of which were statistically significant (p<0.05). Both groups were followed up for 12 months after surgery. Relapse was not observed in any case.
Conclusion: Based on the surgical principles of the open neck-shoulder technique and the understanding of the membrane autopsy in the inguinal region, our center has summarized a set of operation procedures called the “modified neck-shoulder technique” for laparoscopic TEP for tension-free repairs of indirect inguinal hernia. This new surgical technique could quickly and accurately separate and cross the interlayer gap in the preperitoneal space under the enlarged view of the laparoscope, allow the high ligation and detachment or return of the hernia sac, and make the placement of the patch more reliable, reduce collateral damage, reduce postoperative complications, and save operation time.
Spatially Distinct Immunothrombotic Signatures in Patients with Pneumonia-Related Acute Respiratory Distress Syndrome: Insights from Lung Alveolar and Blood Circulation Profiles
Authors: Zong Xiaolong , Geng Liying , Liu Yaru , Wang Xiao , Li Duanyang , Sun Yu , Wang Jinying , Zhou Zhiqing , Li Zhenyu 
Published: September 2023

Abstract
Background
The role of immunothrombosis in the pathogenesis of pulmonary acute respiratory distress syndrome (ARDS) is increasingly recognized, but its implications in extrapulmonary complications remain inadequately understood. This study aimed to compare the immunothrombotic signatures in patients with pneumonia-related ARDS (p-ARDS) at both pulmonary and systemic levels and to assess their clinical relevance.
Methods
This prospective observational study included consecutive patients with p-ARDS admitted to the intensive care unit between July and November 2022. Concurrently hospitalized patients with common pneumonia in the general ward were included as controls. Paired bronchoalveolar lavage fluid (BALF) and serum samples were utilized to quantify 15 biomarkers and characterize pulmonary and systemic immunothrombotic signatures, respectively. The clinical relevance of these biomarkers was explored using Spearman correlation, receiver operating characteristic, and binary logistic regression analyses.
Results
A total of 23 patients with p-ARDS and 10 pneumonia controls were included for analysis. Among the p-ARDS cohort, 10 out of 23 patients experienced mortality within 28 days of admission. Our results revealed significant signatures of pulmonary inflammation and systemic endothelial injury in patients with p-ARDS, in comparison to the pneumonia controls. Specially, BALF IL-6 showed a negative correlation with PaO2/FiO2 ratio (Spearman r = − 0.67, P < 0.001), while serum a disintegrin and metalloproteinase with thrombospondin type 1 motif, 13 (ADAMTS-13) and soluble thrombomodulin (TM) exhibited close correlations with SOFA and DIC score. The combination of BALF IL-6 and serum TM showed promise in distinguishing p-ARDS from common pneumonia (area under the curve [AUC] = 0.955; 95% confidence interval [CI]: 0.895 − 1.000). Furthermore, BALF H3cit was significantly associated with 28-day mortality, even after adjusting for the SOFA score upon admission (odds ratio [OR] = 6.71; 95% CI: 1.05 − 42.44; P = 0.043).
Conclusions
This preliminary investigation revealed compartment-specific differences in the immunothrombotic signature between patients with p-ARDS and those with pneumonia alone. These findings provide insights into the pathophysiology underlying p-ARDS and its complications, with potential to facilitate the development of precision medicine approaches for its clinical management.
Research on Improved YOLOv8 Algorithm for Insulator Defect Detection
Authors: Zhang Lin , Li Boqun , Cui Yang , Lai Yushan , Gao Jing 
Published: September 2023

Abstract
With the rapid advancement of artificial intelligence technologies, drone aerial photography has gradually become the mainstream method for defect detection of transmission line insulators. To address the issues of slow recognition speed and low accuracy in existing detection methods, this paper proposes an insulator defect detection algorithm based on an improved YOLOv8s model. Initially, a Multi-scale Large Kernel Attention (MLKA) module is introduced to enhance the model's focus on features of different scales as well as low-level feature maps. Additionally, by employing lightweight GSConv convolution and constructing the GSC_C2f module, the computational process is simplified and memory burden is reduced, thereby effectively improving the performance of insulator defect detection. Finally, an improved loss function using SIoU is adopted to optimize the model's detection performance and enhance its feature extraction capability for insulator defects. Experimental results demonstrate that the improved model exhibits excellent performance in drone aerial photography for insulator defect detection, achieving an mAP of 99.22% and an FPS of 55.73 frames per second. Compared to the original YOLOv8s and YOLOv5s, the improved model's mAP increased by 2.18% and 2.91%, respectively, and the model size is only 30.18MB, meeting the requirements for real-time operation and accuracy.
Outcome of transarterial radioembolization in patients with hepatocellular carcinoma as a first line interventional therapy and after previous transarterial chemoembolization
Authors: Wagenpfeil Julia , Kupczyk Patrick , Bruners Philipp , Siepmann Robert , Guendel Emelie , Luetkens Julian , Isaak Alexander , Meyer Carsten , Attenberger Ulrike , Kütting Daniel 
Published: September 2023

Abstract
Due to lack of data, there is an ongoing debate regarding the optimal interventional therapy of unresectable hepatocellular carcinoma (HCC). The aim of the study is to compare the results of transarterial radioembolization (TARE) as first-line therapy and after prior transarterial chemoembolization (TACE) in these patients. 83 patients were evaluated. 38 patients received at least one TACE session prior to TARE (27 male; mean age 67.2 years; 68.4% stage BCLC B, 31.6% BCLC C); 45 patients underwent primary TARE (33 male; mean age 69.9 years; 40% BCLC B, 58% BCLC C). Clinical (age, gender, BCLC stage, activity (GBq), Child-Pugh status, portal vein thrombosis, tumor volume), and procedural (overall survival (OS), local tumor control (LTC) and progression-free survival (PFS)) data were compared. Regression analysis was performed to evaluate OS, LTC and PFS. No differences were found in OS (95% CI:1.12, P = 0.289), LTC (95% CI:0.003, P = 0.95) and PFS (95% CI:0.4, P = 0.525). Regression analysis revealed a relationship between Child-Pugh Score (P = 0.005), size of HCC lesions (> 10 cm) (P = 0.022) and OS; neither prior TACE (Child Pugh B patients; 95% CI:0.120, P = 0.729) nor number of lesions (> 10; 95% CI:2.930, P = 0.087) correlated with OS. Prior TACE does not affect outcome of TARE in unresectable HCC.
Ligand Based Pharmacophore Modeling, Virtual Screening, Molecular Docking, Molecular Dynamic simulation and In-silico ADMET Studies for the Discovery of Potential BACE-1 Inhibitors
Authors: Shareef Usman , Altaf Aisha , Zargaham Muhammad Kazim , Bhatti Rohail , Ibrahim Ahsan , Zahid Muhammad Ammar 
Published: September 2023

Abstract
Pharmacophore modeling is an innovative technology to explore and extract potential interactions between ligand-protein complexes. On the other hand, virtual screening is an in-silico technique that uses pharmacophore models to analyze extensive databases of compounds or approved drugs to evaluate interactions. These techniques enable to discover, establish, and evaluate therapeutics and other biologically active compounds and also allow the optimization of several hundred and thousand compounds to be tested for interaction against the target protein or receptor, which narrows down the potential molecules that can be used for further studies. Drug repurposing can be done by integrating these techniques into the study design, allowing reduced cost associated with conventional hit and trial testing of compounds, running large databases in shorter duration. The study reported the successful generation and validation of pharmacophore model with subsequent virtual screening. Virtual screening of databases produced 6 hits which were further subjected to in-silico analysis and resulted in identification of anileridine as the potential BACE-1 inhibitor. Anileridine showed significant interaction with one of the important amino acids of the catalytic dyad of the enzyme i.e. Asp32. Furthermore, MD simulations supported the molecular docking and MM-GBSA results and revealed to formation of stable interactions between anileridine and BACE-1. After establishing anileridine as the potential BACE-1 inhibitor procured from already approved drugs, it was subjected to extensive in-silico ADMET studies. Furthermore, the model (AHRRR) can be used to rationally design novel inhibitors of BACE-1 and also identify new molecules from databases as potential BACE-1 inhibitors.
EZH2 Suppresses Ferroptosis in Hepatocellular Carcinoma and Reduces Sorafenib Sensitivity Through Epigenetic Regulation of TFR2
Authors: Lai Yongwei , Han Xu , Xie Bo , Wang Didi , Li Tongtong , Li Wei , Xie Yaohong , Yang Zhengyi , Song Wenqi , Zhang pengxia 
Published: September 2023

Abstract
Background: Sorafenib is a first-line drug, and increasing the sensitivity to sorafenib can effectively prolong the time of sorafenib resistance, which has great benefits for the treatment of patients with hepatocellular carcinoma. One of the important mechanisms of sorafenib in treating HCC is to induce cell ferroptosis. Hence, it is of great importance to investigate the mechanisms that increase sorafenib-induced ferroptosis in hepatocellular carcinoma.
Methods: Bioinformatic analysis approaches were used to assess EZH2 expression, prognosis and clinical characteristics. IHC staining assays were performed to assess the expression of EZH2 in tumors and normal tissues. CCK-8, EdU staining and colony formation assays were performed to assess cell proliferation. Western blot and q-PCR analysis of ferroptosis-associated genes. As indicators of ferroptosis, intracellular levels of glutathione, malondialdehyde, and iron were measured. ROS detection by the DCFH-DA probe and mitochondrial membrane potential were tested by JC-1. ChIP analysis was used to study histone modification. The antitumor effects of the combination of tazemetostat and sorafenib were investigated in both in vitro and in vivo studies.
Results: EZH2 expression is upregulated in HCC and predicts an unfavorable prognosis. Overexpression of EZH2 can promote HCC cell proliferation and reduce ferroptosis. Further analysis showed that EZH2 can increase the modification of H3K37me3 and regulate the expression of TFR2. Reducing RNA polymerase II binding in the promoter region of TFR2 leads to decreased expression of TFR2. The decrease in intracellular iron levels inhibits the occurrence of the Fenton reaction and cell ferroptosis. Knockdown of EZH2 can increase sorafenib sensitivity in hepatocellular carcinoma cells, and tazemetostat, an inhibitor of EZH2, with sorafenib had significant synergistic antitumor effects both in vitro and in vivo
Conclusions: EZH2 epigenetically regulates TFR2 expression through H3K27me3 and inhibits the occurrence of ferroptosis. The EZH2 inhibitor tazemetostat synergized with sorafenib and had superior synergistic effects in anticancer therapy in vitro and in vivo.
The Generalized AEP for Higher Order Nonhomogeneous Markov Information Source
Authors: Shi Zhiyan , Zhu Xiaoyu 
Published: September 2023

Abstract
The asymptotic equipartiton property (AEP) plays a crucial role in information theory, providing a theoretical foundation for understanding and analyzing aspects such as coding, compression, and the reliability of communication systems. In this paper, we mainly study the generalized AEP of higher order nonhomogeneous Markov information sources by establishing several strong deviation theorems. To achieve this, we first introduce the concepts of generalized sample divergence rate of th-order nonhomogeneous Markov information sources. Meanwhile, we give a class of generalized strong deviation theorems for moving average of the functions of + 1 variables for th-order nonhomogeneous Markov information sources, and also establish strong deviation theorems and strong limit theorems of the frequencies of occurrence of ordered tuples of states for this Markov information sources. Finally, the generalized AEP for th-order nonhomogeneous Markov information sources are establised. Our results also generalize some known results.
MSC(2020): 60J10; 60F15; 94A15