bluuebunny/crossref_metadata_2025_split huggingface dataset:
CEP55 as a promising biomarker and therapeutic target on gallbladder cancer
Authors: Huang Maotuan , Zhong Fuxiu , Chen Mingyuan , Hong Lingju , Chen Weihong , Abudukeremu Xiahenazi , She Feifei , Chen Yanling 
Published: May 2023

IntroductionGallbladder cancer (GBC) is a highly malignant biliary tumor with a poor prognosis. As existing therapies for advanced metastatic GBC are rarely effective, there is an urgent need to identify more effective targets for treatment.MethodsHub genes of GBC were identified by bioinformatics analysis and their expression in GBC was analyzed by tissue validation. The biological role of CEP55 in GBC cell and the underlying mechanism of the anticancer effect of CEP55 knockdown were evaluated via CCK8, colony formation assay, EDU staining, flow cytometry, western blot, immunofluorescence, and an alkaline comet assay.ResultsWe screened out five hub genes of GBC, namely PLK1, CEP55, FANCI, NEK2 and PTTG1. CEP55 is not only overexpressed in the GBC but also correlated with advanced TNM stage, differentiation grade and poorer survival. After CEP55 knockdown, the proliferation of GBC cells was inhibited with cell cycle arrest in G2/M phase and DNA damage. There was a marked increase in the apoptosis of GBC cells in the siCEP55 group. Besides, in vivo, CEP55 inhibition attenuated the growth and promoted apoptosis of GBC cells. Mechanically, the tumor suppressor effect of CEP55 knockdown is associated with dysregulation of the AKT and ERK signaling networks.DiscussionThese data not only demonstrate that CEP55 is identified as a potential independent predictor crucial to the diagnosis and prognosis of gallbladder cancer but also reveal the possibility for CEP55 to be used as a promising target in the treatment of GBC.
Real world evidence of Lenvatinib + anti PD-1 as an advanced line for metastatic melanoma
Authors: Stoff Ronen , Asher Nethanel , Laks Shachar , Steinberg Yael , Schachter Jacob , Shapira-Frommer Ronnie , Grynberg Shirly , Ben-Betzalel Guy 
Published: May 2023

IntroductionImmunotherapy has revolutionized the prognosis of patients with metastatic melanoma. To date, the most active regimen is the combination of ipilimumab + nivolumab (ipi-nivo) achieving a response rate of nearly 60% and a median survival (OS) of 6 years. However, approximately 40% of patients experience primary resistance, while around 50% experience secondary resistance, highlighting the need for an effective second-line treatment option The recently published results on the use of lenvatinib + pembrolizumab in the advanced line setting led to the adoption of this regimen at our institution. Here we present our experience with this regimen, focusing on efficacy and safety.MethodsElectronic medical records of patients treated at a tertiary referral melanoma center, with at least one cycle of anti PD-1 + lenvatinib from 2020 to 2023 were analyzed for baseline demographic characteristics, disease related characteristics and treatment outcomes.ResultsForty-two patients were identified. The Response rate (RR) was 28% and the disease control rate was 38%. Responses were seen across different melanoma subtypes, including 67% in acral melanoma, 20% in uveal melanoma, and 25% in mucosal melanoma. Patients with a more aggressive disease manifested by elevated lactate dehydrogenase (LDH) achieved a RR of 26%, while patients with active central nervous system (CNS) metastases had a RR of 31%, and an intra-cranial RR of 23%. Responses were seen across lines of treatment, with a 25% RR in the second and third lines, and a 36% RR in the fourth and fifth lines. The median progression free survival was 3 months, and the median survival was 11 months. The treatment was not easily tolerated with 31% of the patients experiencing grade 3-4 toxicity, which was manageable through dose interruptions and reductions. Only 7% of patients discontinued the treatment due to toxicity.ConclusionLenvatinib in combination with anti-PD1 had demonstrated both relative safety and efficacy in patients with metastatic melanoma of all subtypes in the advanced line setting. We are eagerly anticipating the mature results of the LEAP-004 study hoping that this regimen will receive regulatory approval, paving the way for its widespread adoption in daily practice worldwide.
Performance of two interferon-gamma release assays for tuberculosis infection screening in Kawasaki children before immunosuppressive therapy
Authors: Chen Hao , Zheng Huiwen , Cui Lang , Xiao Jing , Li Feina , Wang Yonghong , Guo Yajie , Chen Yuying , Yuan Yue , Shen Chen 
Published: May 2023

ObjectiveWe aimed to compare QuantiFERON-TB Gold In-Tube (QFT-GIT) and X.DOT-TB for screening latent tuberculosis infection (LTBI) in kawasaki patients, and to identify the risk factors associated with indeterminate IGRA results.MethodsWe conducted a retrospective study on children with KD, who were screened for mycobacterium tuberculosis (Mtb) infection by either ELISA-based QFT-GIT or ELISPOT-based X.DOT-TB tests, admitted in Department of Cardiology, Beijing Children's Hospital from July 2019 to April 2022.ResultsA total of 1327 cases were included. Among them, 932 cases were tested by QFT-GIT and 395 cases by X.DOT-TB. The positive rate of children was 0.1% and 0.2%, and the indeterminate rate was 68.2% and 6.1% for QFT-GIT and X.DOT-TB, respectively. Patients with hypoproteinemia had a higher risk of indeterminate X.DOT-TB result. Female, critical ill, shock or hypoproteinemia presented statistically significant associations with an increased risk of indeterminate QFT-GIT result. High-dose of IVIG inhibited the release of IFN-γ by more than 90%, which might account for the high indeterminate incidence.ConclusionIt is recommended to perform X.DOT-TB rather than QFT-GIT to screen LTBI in patients with high level of the mitogen that can inhibit IFN-γ release. For KD children with positive IGRA results, it has a higher risk of activation TB infection when treated with immunosuppressive therapy in the future. Children with KD aged &lt;5 years old had higher frequency of indeterminate IGRA results.
Photo-thermoelastic wave propagation with changing thermal conductivity on excited pulsed laser nanoscale microelongated semiconductor material
Authors: Farhan Ashraf M. , El-Sapa Shreen , El-Bary Alaa A. , Chteoui Riadh , Lotfy Khaled 
Published: May 2023

Indroduction: The novel model of a non-local elastic semiconductor material that is microelongated is created. The process of photothermal transfer is responsible for the stimulation of the material. Photo-thermoelastic theories are used when the thermal conductivity is changed for the non-local medium. Under the influence of laser pulses, the effective framework describes the nanoscale microelongation instance as well as the interference between the photo-thermoelastic propagation waves in the non-local medium. It is possible to think of thermal conductivity as a linear function of temperature when electronic and thermoelastic deformation mechanisms are described. Two-dimensional deformation (2D) is used to extract the main fields, which obtained in non-dimension.Methods: Harmonic wave analysis, which is described by the normal mode, has been used to convert the basic equations into non-homogeneous higher-order ordinary differential equations. Applying a small subset of the possible non-local semiconductor surface conditions leads to exhaustive solutions.Result and Discussion: The outcomes of numerical simulations for silicon (Si) are graphically shown. There are comparisons made and explanations given for the investigated physical factors like their thermal conductivity, laser pulses and microelongation parameters.
Oral CBD-rich hemp extract modulates sterile inflammation in female and male rats
Authors: Hopkins Shelby , Kelley Tel , Roller Rachel , Thompson Robert S. , Colagiovanni Dorothy B. , Chupka Kris , Fleshner Monika 
Published: May 2023

Introduction: Cannabidiol (CBD) extract from the cannabis plant has biomedical and nutraceutical potential. Unlike tetrahydrocannabinol (THC), CBD products produce few psychoactive effects and pose little risk for abuse. There is emerging preclinical and clinical evidence that CBD is stress modulatory and may have anti-inflammatory properties. People across the United States legally ingest CBD-rich hemp extracts to manage mental and physical health problems, including stress and inflammation. Preclinical studies have revealed potential mechanisms for these effects; however, the impact of this prior work is diminished because many studies: 1) tested synthetic CBD rather than CBD-rich hemp extracts containing terpenes and/or other cannabinoids thought to enhance therapeutic benefits; 2) administered CBD via injection into the peritoneal cavity or the brain instead of oral ingestion; and 3) failed to examine potential sex differences. To address these gaps in the literature, the following study tested the hypothesis that the voluntary oral ingestion of CBD-rich hemp extract will attenuate the impact of stressor exposure on plasma and tissue inflammatory and stress proteins in females and males.Methods: Adult male and female Sprague Dawley rats (10–15/group) were randomly assigned to be given cereal coated with either vehicle (coconut oil) or CBD-rich hemp extract (L-M0717, CBDrx/Functional Remedies, 20.0 mg/kg). After 7 days, rats were exposed to a well-established acute model of stress (100, 1.5 mA, 5-s, intermittent tail shocks, 90 min total duration) or remained in home cages as non-stressed controls.Results: Stressor exposure induced a robust stress response, i.e., increased plasma corticosterone and blood glucose, and decreased spleen weight (a surrogate measure of sympathetic nervous system activation). Overall, stress-induced increases in inflammatory and stress proteins were lower in females than males, and oral CBD-rich hemp extract constrained these responses in adipose tissue (AT) and mesenteric lymph nodes (MLN). Consistent with previous reports, females had higher levels of stress-evoked corticosterone compared to males, which may have contributed to the constrained inflammatory response measured in females.Discussion: Results from this study suggest that features of the acute stress response are impacted by oral ingestion of CBD-rich hemp extract in female and male rats, and the pattern of changes may be sex and tissue dependent.
The relationship of muscle oxygen saturation analyzer with other monitoring and quantification tools in a maximal incremental treadmill test
Authors: Osmani Florent , Lago-Fuentes Carlos , Alemany-Iturriaga Josep , Barcala-Furelos Martín 
Published: May 2023

Introduction: The study aims to explore whether NIRS derived data can be used to identify the second ventilatory threshold (VT2) during a maximal incremental treadmill test in non-professional runners and to determine if there is a correlation between SmO2 and other valid and reliable exercise performance assessment measures or parameters for maximal incremental test, such as lactate concentration (LT), RPE, HR, and running power (W).Methods: 24 participants were recruited for the study (5 women and 19 men). The devices used consisted of the following: i) a muscle oxygen saturation analyzer placed on the vastus lateralis of the right leg, ii) the Stryd power meter for running, iii) the Polar H7 heart rate band; and iv) the lactate analyzer. In addition, a subjective perceived exertion scale (RPE 1-10) was used. All of the previously mentioned devices were used in a maximal incremental treadmill test, which began at a speed of 8 km/h with a 1% slope and a speed increase of 1.2 km/h every 3 min. This was followed by a 30-s break to collect the lactate data between each 3-min stage. Spearman correlation was carried out and the level of significance was set at p &lt; 0.05.Results: The VT2 was observed at 87,41 ± 6,47% of the maximal aerobic speed (MAS) of each participant. No relationship between lactate data and SmO2 values (p = 0.076; r = −0.156) at the VT2 were found. No significant correlations were found between the SmO2 variables and the other variables (p &gt; 0.05), but a high level of significance and strong correlations were found between all the following variables: power data (W), heart rate (HR), lactate concentration (LT) and RPE (p &lt; 0.05; r &gt; 0.5).Discussion: SmO2 data alone were not enough to determine the VT2, and there were no significant correlations between SmO2 and the other studied variables during the maximal incremental treadmill test. Only 8 subjects had a breakpoint at the VT2 determined by lactate data.Conclusion: The NIRS tool, Humon Hex, does not seem to be useful in determining VT2 and it does not correlate with the other variables in a maximal incremental treadmill test.
Rosemary and neem methanolic extract: antioxidant, cytotoxic, and larvicidal activities supported by chemical composition and molecular docking simulations
Authors: Alhaithloul Haifa A. S. , Alqahtani Mesfer M. , Abdein Mohamed A. , Ahmed Mohamed A. I. , Hesham Abd El-Latif , Aljameeli Mohammad M. E. , Al Mozini Reem N. , Gharsan Fatehia N. , Hussien Suzan M. , El-Amier Yasser A. 
Published: May 2023

This study aimed to employ GC–MS to assess the chemical composition of MeOH leaf extracts of R. officinalis and A. indica and evaluate their insecticidal, antioxidant, and antibacterial activities. Twelve components, representing 98.61% and 100% of the total volatile compounds, were deduced from the extracted R. officinalis and A. indica, respectively, using this method. In R. officinalis extract, limonene is typically positioned as the main component (23.03%), while the main chemicals identified in A. indica extract were methyl (E)-octadec-13-enoate (23.20%) and (2R)-1,3,8-trimethyl-4-propyl-5-ethyl-2-(1-hydroxyethyl)-7-methoxycardonylethyl-6-methylenecarbonyl-porphyrin (23.03%). Both extracts of R. officinalis and A. indica exhibited different toxicity against the stored grain pest T. castaneum, with LC50 values of 1.470 and 2.588 mg/ml, respectively. Additionally, after 4 and 5 h of treatment at a concentration of 0.2 mg/ml, the A. indica extract showed the highest levels of repellent action (81.4% and 93.4%), and the R. officinalis extract showed a good repellent rate (64.9% and 80.7%) against T. castenum larvae. With an IC50 value of 35.83 and 28.68 mg/L and a radical scavenging activity percentage of 67.76% and 72.35%, the leaf extract was found to be the most potent plant extract when tested for DPPH antioxidant activity. Overall results showed that MeOH extracts of R. officinalis and A. indica were more effective against S. aureus than E. coli. To determine how the investigated chemicals attach to the active sites of E. coli DNA gyrase A and S. aureus undecaprenyl diphosphate synthase, docking studies were carried out. The consensus score analysis showed that limonene exhibits the best binding energy with both enzymes in docking analysis and more stability in molecular dynamics simulations. The RMSD was obtained at 20.6 and 4.199 (Kcal/mole). The two compounds were successfully used in molecular dynamics simulation research to generate stable complexes with DNA gyrase A.
The impact of multiple language exposure on cognition during childhood: evidence from the UK Millennium Cohort Study
Authors: Clayden Jonathan D. , Hope Steven , Argyri Froso , Goksan Sezgi , Stefani Artemis , Wei Li , Liegeois Frederique Jeanne 
Published: May 2023

IntroductionMany studies argue that exposure to, and use of, multiple languages in childhood has beneficial effects beyond the linguistic domain, including on executive functions (EFs), although recent evidence remains controversial. EFs encompass abilities necessary for regulating goal-directed behaviours in everyday life and, in children, EFs strongly predict later academic achievement and wellbeing. One theoretical framework distinguishes “hot” EFs, which have a reward or affective component, from “cool” EFs that do not. How exposure to more than one language in early childhood modulates hot and cool EFs in later childhood, alongside other environmental and cognitive factors, remains poorly understood.MethodsWe analysed data from the UK Millennium Cohort Study, a large-scale, nationally representative longitudinal cohort study, which provides information on perinatal and environmental factors (e.g., languages spoken in the home, maternal education) alongside cognitive measures assessed in English. At 3 years, we examined the effect of multiple language exposure on the Bracken school readiness assessment (knowledge of shapes, letters, etc.), and on naming vocabulary. At age 11, we examined the predictors of cool EF, measured with a spatial working memory task; hot EF, measured using a gambling task; and vocabulary, measured using a verbal reasoning task.ResultsData from 16,134 children were analysed. At age 3, a negative effect of multiple language exposure on school readiness and vocabulary was observed, but the difference was smaller with higher maternal education. At age 11, there was also a negative effect on vocabulary, but smaller than that observed at age 3. There were no direct effects of language exposure on either spatial working memory or gambling scores. For hot EF, the multiple language exposure effects were indirect, mediated by early cognition, and the most significant predictor of gambling strategy was sex. For cool EF, school readiness and vocabulary at age 3 were the strongest predictors.DiscussionOur findings, based on a UK population sample, highlight the importance of considering socioeconomic status and early-life abilities when interpreting the effects of language environments on hot and cool EFs.
A bibliometric analysis of publications on obsessive–compulsive disorder using VOSviewer
Authors: Tang Yimiao , Lu Xuewen , Wan Xin , Hu Maorong 
Published: May 2023

BackgroundObsessive–compulsive disorder (OCD) is one of the top ten disabling diseases seriously affecting the health of population. Recently, studies on this disease significantly increased. However, only a few bibliometric analyses concerning this area have been reported. In this study, we used bibliometrics and visualization tools to examine the current state, hot topics and future trends in OCD research.MethodsScientific publications regarding OCD were retrieved from the Web of Science Core Collection (WoSCC) database. The features of OCD research were further analyzed using VOSviewer.ResultsA total of 24,552 publications and 65,296 authors in the field of OCD were retrieved from 2000 to 2022, showing an overall upward trend in publications over the past 22 years. One hundred and thirteen countries around the world had participated in the research. Among these countries, the developed countries such as the United States, England, and Canada were the crucial productive nations in this subject. As for institutions, the Harvard University, the University of London, and the University of California system were the leading institutions. Authors including Storch EA, Mataix-Cols D, and Stein DJ were the prolific authors. 1,949 journals are contributing to the OCD field, of which the top three are Biological Psychiatry (831 articles), European Neuropsychopharmacology (776 articles) and Psychiatric Research (648 articles). Research hotspots of OCD included pathogenesis, epidemiology, comorbidities, clinical features, and evaluation methods. COVID-19, mental health, functional connectivity, and genome-wide association were emerging trends in the field of OCD.ConclusionThis study integrates the bibliometric information on the current research status and emerging trends in OCD from a macro perspective. The findings can provide valuable insights into further research on OCD.
Exploring the positive aspects of caregiving among family caregivers of the older adults in India
Authors: Mishra Nidhi , Datti Ravi S. , Tewari Ashutosh , Sirisety Maneela 
Published: May 2023

BackgroundCaregiving is a committed role that focuses on providing the required support and care to a care receiver who is either ill, disabled, or dependent to the extent that they are incapable of independent functioning. The topic of caregiving has been extensively studied worldwide, mainly focused on the negative aspects of caregiving, like caregiver stress, burden, role conflicts, and burnout among caregivers. However, limited efforts have been made to understand the positive aspects of caregiving among informal caregivers who spend most of their time in this unpaid role. The present study addresses this concern by exploring the positive aspects of caregiving among family caregivers of older persons in India.MethodologyThis is a qualitative study, which was conducted, using the existential-phenomenological approach. In this study, a purposive sampling technique was used, and a total of a 100 family caregivers participated from four cities in India, namely Prayagraj, Pune, Visakhapatnam, and Guwahati. Twenty-five family caregivers between the age of 30–50  years participated from each of these four cities. The data was collected using six semi-structured interview questions on positive aspects of caregiving based on the lived experiences of caregivers. The interview schedule was developed based on the PERMA model and finalized after the pilot study. Each interview took 30–50 min and was recorded and transcribed.ResultsThe data was analyzed using thematic analysis. Some commonalities and differences were observed in the responses given by family caregivers from the four cities. Four major themes that emerged for the “Nature of positive caregiving” among participants from all four cities are “Caregiver’s attitude,” “Care and compassion,” “Roles and responsibilities,” and “Beliefs and values.” Four significant themes for the “Factors contributing to positive caregiving” are “Meaningfulness in life,” “Sense of belongingness,” “Personal growth,” and “Empathetic understanding.”ConclusionBased on the study’s findings, it can be said that the themes were associated with the PERMA model. Positive caregiving is an important indicator of caregivers’ and care receivers’ well-being. The findings have implications for planning some action research, training, and counseling programs for promoting positive aspects of caregiving among informal caregivers.