bluuebunny/crossref_metadata_2025_split huggingface dataset:
Osterix mRNA Enrichment in Small Extracellular Vesicles Derived From Osteogenically Induced ADSCs: A Promoter of Osteogenic Differentiation in BMSCs
Authors: Liang Zhaoquan , Wu Yuelin , Bao Junhao , Xiao Qiang , Luo Sidong , Liu Xinfang , Wang Yeyang , Xie Chao , Zhang Li 
Published: January 2025

ABSTRACTOsteogenic differentiation of bone marrow stem cells (BMSCs) is essential for bone tissue regeneration and repair. However, this process is often hindered by an unstable differentiation influenced by local microenvironmental factors. While small extracellular vesicles (sEVs) derived from osteogenically induced adipose mesenchymal stem cells (ADSCs) reportedly can promote osteogenic differentiation of BMSCs, the underlying molecular mechanisms remain incompletely understood. In this study, we investigated the mRNA expression profile of ADSC‐sEVs+ and explored the role of specific mRNAs in the osteogenic differentiation of BMSCs. We first validated the osteogenic induction activity of ADSC‐sEVs+ through both in vitro and in vivo experiments. Using reverse transcription polymerase chain reaction, we compared mRNA expression between ADSC‐sEVs+ and ADSC‐sEVs and further assessed the impact of specific mRNAs on the differentiation of BMSCs through a series of in vitro experiments. One of our key findings was that osterix mRNA was highly enriched in ADSC‐sEVs+, which significantly enhanced alkaline phosphatase staining and upregulated downstream osteoblastic markers in BMSCs. Both overexpression and knockdown experiments confirmed that osterix mRNA is a critical signalling molecule that facilitates the differentiation of BMSCs into osteoblasts through ADSC‐sEVs+. This finding expands our understanding of the molecular mechanisms underlying the osteogenic differentiation of BMSCs and offers a promising strategy for targeted osteoblastic differentiation in clinical applications.
Measuring the Work Relative Value of Common Nursing Items for the General Departments of Tertiary Hospitals: A Descriptive Study
Authors: Wang Yingli , Wang Cong , Ren Hongfei , Mao Ling , Chen Fenjiao , Liang Taoyun , Luo Yuqin , Jiang Yan 
Published: January 2025

ABSTRACTAim(s)This study aims to evaluate the workload of clinical nurses by measuring the work relative value (work RVU) of common nursing items based on the resource‐based relative value scale in China.BackgroundVarious single measurements have been employed to measure the nursing workload, but no comprehensive method has yet to be developed in China.MethodsA descriptive study was conducted to construct a common item set for nursing work in general wards on the basis of the 2019 History Information System nursing database from Class A tertiary hospitals to identify the time associated with each service. Data cleaning methods, expert consensus meetings, observational methods, Delphi consensus methods and the analytic hierarchy processes were employed.ResultsA common item set for nursing work was established, which contained 2 first‐level indexes, 8 secondary‐level indexes and 79 third‐level indexes. The average working hours of the common items ranged from 1.38 to 23.76 min. The top three nursing items identified were: extra large dressing change, cleaning enema, and nasal feeding catheterization.ConclusionsThe common nursing items used in general wards were universal. The work RVU of common items comprehensively evaluates clinical nurses' workload, which can better meet the needs of management.ImplicationsThe work RVU of common items will facilitate effective and comprehensive allocation of nursing human resources and performance assessment.Reporting MethodWe have adhered to relevant EQUATOR guidelines and named the reporting method.
Nursing Students' Attitudes Towards Clinical Practice and Occupational Risk Perceptions
Authors: Daşbilek Ferhat , Yeşiltepe Akgün , Erçel Şeyda 
Published: January 2025

ABSTRACTAims and ObjectivesIn this study, it was aimed to determine nursing students' attitudes towards clinical practice and their perceptions of occupational risk.MethodThe research is descriptive and cross‐sectional. The population of this study consisted of second‐, third‐ and fourth‐year students studying in the Department of Nursing affiliated to the Faculty of Health Sciences of a university located in Turkey. The analysis was conducted using JASP 0.19.1.0 and SPSS 25 software. Mann–Whitney U and Kruskal–Wallis tests were applied. The Bonferroni test was performed to identify the group causing the difference.ResultsA significant positive correlation was found between nursing students' perception of occupational risk and nursing students' attitude toward clinical practice (r = 0.434, p = 0.000). The mean score of the nursing students' perception of occupational risk in nursing students was 68.74 ± 15.04. The mean score of attitude towards clinical practice in nursing students was 100.04 ± 24.83. A statistically significant difference was found between the presence of attitude towards clinical practice in nursing students and perception of occupational risk in nursing students in the gender variable (p < 0.05), whereas a statistically significant difference was found between adequate information about the nursing profession and attitude towards clinical practice in nursing students (p < 0.05).ConclusionIn the findings of the study, nursing students' perception of occupational risk and attitudes towards clinical practice were found at a high level. A statistically significant difference was found between the presence of perception of occupational risk in nursing students and attitude towards clinical practice in nursing students in the gender variable. In addition, a statistically significant difference was found between adequate knowledge and nursing profession about the nursing profession and attitude towards clinical practice in nursing students.
Longitudinal Serotonergic and Dopaminergic Binding: Impact on Parkinson's Disease Progression and Levodopa Dyskinesia
Authors: Jeong Eun Hye , Lee Jae Yong , Song Yoo Sung 
Published: January 2025

ABSTRACTBackground and PurposeWe investigated the relationship between serotonergic and dopaminergic specific binding transporter ratios (SBRs) over 4 years in Parkinson's disease (PD) patients. We assessed serotonergic innervation's potential compensatory role for dopaminergic denervation, association with PD symptoms, and involvement in the development of levodopa‐induced dyskinesia (LID).MethodsSBRs of the midbrain and striatum were evaluated from [I‐123] N‐ω‐fluoropropyl‐2β‐carbomethoxy‐3β‐(4‐iodophenyl)nortropane SPECT images at baseline and after 4 years. Correlations between SBRs and PD symptoms were analyzed, alongside interval changes.ResultsStudy included 177 PD patients (110 males, 67 females; mean age 61.0 ± 9.0 years). Significant worsening was observed in Hoehn and Yahr staging and Movement Disorder Society‐Unified Parkinson's Disease Rating Scale (MDS‐UPDRS) Part II and III scores over 4 years (p < 0.05, p < 0.001, and p < 0.001, respectively). SBRs of the caudate, putamen, and midbrain declined significantly (p < 0.001). Midbrain and striatal SBRs correlated significantly at both baseline and 4‐year follow‐up (p < 0.0001). Striatal SBRs correlated significantly with MDS‐UPDRS II and III scores at both time points, while midbrain SBRs correlated with changes in MDS‐UPDRS III scores over the 4 years (p < 0.01). Putamen and midbrain SBRs at 4 years were significantly lower in patients who developed LID compared to those who did not (p < 0.05).ConclusionThe study demonstrates correlations between midbrain and putamen SBRs and MDS‐UPDRS scores over 4 years in PD patients. Midbrain serotonin dysfunction may contribute to the development of LID.
The effect of music on fistula needle insertion‐related pain intensity and pain distress: A single‐centre, single‐blind, randomised controlled trial
Authors: Baykan Sultan , Kılıç Akça Nazan 
Published: January 2025

AbstractBackgroundPatients feel pain and distress when a needle is inserted into the fistula. This affects the comfort of patients. One of the nonpharmacological methods used to decrease pain and stress is music intervention.ObjectiveThis study was conducted to assess the effect of Turkish classical music in makams, which the patients preferred, via headphones on fistula needle insertion‐related pain intensity and pain distress.DesignIt was single‐blind randomised controlled study.ParticipantsThis study was conducted with 54 patients between March and June 2023.MeasurementsThe patient data were collected through a patient information form, visual analogue scale and distress thermometer. The intervention group was played music for 10 min, starting 5 min before the needle was inserted into the fistula. The music intervention was made in accordance with the established music protocol. The control group received only routine treatment.ResultsPatients in the intervention group were found to have a significant decrease in fistula needle insertion‐related pain intensity and pain distress.ConclusionAccording to the results obtained from the research, music listening to patients is a reliable, effective and low‐cost way of a nursing intervention to reduce levels of fistula needle insertion‐related pain intensity and pain distress. Additionally, the results will provide evidence‐based guidance to dialysis nurses for pain and distress management.
Generating Value by Working With User Communities: An Analysis of Financial Market Returns to Corporate Open Source Code Contributions
Authors: Aksoy‐Yurdagul Dilan 
Published: January 2025

ABSTRACTUsers are an important source of innovation. Scholars suggest that established firms can gain product‐related insights by working with user communities and studies documenting various ways of working with users, as well as managers' interest in doing so. However, the link between working with user communities for product development purposes and its value for firms is not established. Coupling the use of event study methodology and regression analysis, I examine stock market reactions to corporate announcements stating that the firm is contributing software code to the community. I find that when firms state that generating insights from users regarding new and improved features and functionality is a motivation for contributing code, the market's reaction to the announcement is greater than for announcements that do not state this goal. Additional analysis provides evidence supporting the hypothesis that firms can and do benefit by working with user communities and achieve increased R&D efficiency, which leads to greater firm value.
Estimation of water consumption using artificial neural network: A study on subscribers in Kocaeli Province, Turkey
Authors: Görenekli Kasım , Gülbağ Ali 
Published: January 2025

AbstractThis study focuses on Kocaeli province in Turkey and employs Artificial Neural Networks (ANN) to predict monthly water consumption. With approximately 800.000 water subscribers, a sample of 1.200 subscribers from residential, commercial and official categories was selected for analysis. The dataset includes water consumption records, weather information, weekends and holidays, the previous month's consumption and the influence of the COVID‐19 pandemic. During an extensive 80‐month period, spanning from January 2016 to August 2022, our study thoroughly analysed the dataset, ultimately achieving a notable R2 values of 0.88 for official subscribers and 0.87 for commercial subscribers, respectively. The findings of this research are highly important, considering Turkey's high water loss rate of approximately 37% (SABAH, n.d., 2022) and Turkey's per capita water supply is below the global average (“Precipitation by country, around the world”, n.d.). By leveraging artificial intelligence techniques in estimating water consumption, this study provides meaningful insights for effective water resource management and allocation.
Exploring the gaps: A scoping review of burn injury research in skin of colour
Authors: Nguyen Antoinette , Duckworth Emily , Abu‐Romman Anmar , Melnick Bradley , Coles Brigid , Galiano Robert D. 
Published: January 2025

AbstractBurn injury management and outcomes reveal observed disparities in individuals with darker skin tones, likely influenced by limited representation in medical literature and clinical research. These gaps may contribute to variations in care quality and outcomes for these populations. A comprehensive literature search was conducted across PubMed, Scopus, and Embase databases, initially yielding 74 articles. Due to limited relevant studies directly addressing the research question, the approach shifted from a systematic review to a scoping review to allow for a broader exploration of potential disparities in burn injury outcomes. Following these criteria, 31 relevant articles were identified and analysed. The analysis suggests an underrepresentation of diverse skin tones in medical textbooks and clinical research, limitations in current burn assessment tools for darker skin, and a lack of tailored treatment protocols. Studies indicate that patients with darker skin tones may face higher risks of complications and varied outcomes, potentially influenced by systemic healthcare challenges and limited guidelines addressing diverse skin types. This scoping review highlights the importance of more inclusive research and clinical practices that consider the specific needs of individuals with darker skin tones. Addressing these observed gaps can support improvements in burn injury management, ultimately contributing to more equitable healthcare for all skin types.
Experimental Validation of a Predictive Control Co-Design Algorithm
Authors: Nash Austin L. 
Published: January 2025

Abstract
Control co-design (CCD) represents a promising solution for coordinating the physical design and control of dynamic engineering systems as technological demands become more stringent. Predictive control co-design (pCCD), recently introduced to the CCD literature, optimizes combinations of feedforward and feedback static gain sets at the system design stage to combine the robustness and preview control afforded using state-of-the-art control methods, like model predictive control (MPC), in CCD with the computational efficiency of open-loop CCD methods that solve CCD problems with a single optimization level. This work contributes the first experimental validation of pCCD to the literature. First, pCCD is performed offline on a spring-mass-damper system. The co-designed system’s optimal response is then experimentally validated online. Results are compared to an analogous system co-designed with an open-loop CCD method. The experimental system co-designed using pCCD yielded a sum squared error with respect to a desired reference signal 40 times smaller than the system co-designed using open-loop CCD. The results indicate that pCCD yields co-designed systems with superior online robustness in comparison to open-loop CCD methods. Moreover, systems co-designed using pCCD are more robust to both modeling error and unexpected disturbance inputs or changes in desired reference signals encountered online.
Precision Quaternion Estimation With Partially Norm-Constrained Unscented Kalman Filtering
Authors: Mozaffari Hamed , Dabiri Arman 
Published: January 2025

Abstract
Accurate orientation estimation is a key challenge in dynamics and control, particularly for rigid body motion. Quaternions are commonly used to represent rotations due to their computational efficiency, but they must always maintain a unit norm to function correctly. If this constraint is not enforced properly, it can lead to significant errors in orientation estimation. This article proposes an unscented Kalman filter that ensures the quaternion parameters, a subvector within the state vector, adhere to the unit norm constraint. The proposed method provides a closed-form solution without dividing the state vector into constrained and unconstrained vectors. In simulation studies under high-noise conditions, the filter demonstrates significantly improved performance compared to standard unscented and pseudo-unscented Kalman filters, enhancing accuracy during both transient and steady-state phases. These results highlight the importance of enforcing quaternion constraints to reduce mean square error and improve convergence.
Hydro Power Generation in India – Status and Challenges
Authors: Thareja Dharam Vir , Bawa Jaideep Singh 
Published: January 2025

Power generation in India has come a long way from just about 1. 3 GW at the time of independence in 1947 to more than 416 GW as on the end of financial year 2022-23 (April 1, 2022 to March 31, 2023), reflecting an increase of more than 300 times. As a part of this generation, the hydro-power during this period of more than 75 years has increased almost 100 times from less than 0.5 GW to about 52 GW.
The annual electricity generation in India has increased from just about 805 BU during the year 2009-10 to more than 1624 MU during the year 2022-23 (Figure 3.1) and it has been estimated to touch about 2280 BU by the year 2029-30, as per the projections of 20th Electric Power Survey (EPS) of India conducted by Central Electricity Authority (CEA). The peak power demand of India is also growing which touched around 216 GW during the year 2022-23 and is projected to increase to about 334 GW by the year 2029-30.
Faculty perceptions of a professional development program for developing CUREs and promoting inclusive and equitable teaching
Authors: Gough Laura , Miranda Rommel , Hemm Matthew , Norman Leann , Jara Brian 
Published: January 2025

ABSTRACT

The Diffusion of Innovations (DOI) model can be used to explore how faculty prioritize learning about and adopting new pedagogical approaches. Here, we use the DOI framework to contextualize biology faculty perceptions of a professional development (PD) program designed to help them create a full semester course-based undergraduate research experience (CURE) class at a large, public comprehensive university. PD sessions included exploring self-reflexive identity while fostering inclusive classroom spaces through understanding and interrupting implicit bias and microaggressions. This qualitative study sought to determine 11 biology faculty members’ beliefs about the influence of their year-long PD on their CURE development and teaching practices. Findings suggest that faculty were motivated to teach CUREs for a variety of reasons. A common incentive was integrating research into a CURE to bring their passion into their classroom and to engage more students in research. This may be particularly important at institutions where faculty have a heavy teaching load. Faculty also reported modifying their teaching in their CUREs and other courses to be more inclusive and equitable. The importance of peer interactions in the PD was emphasized repeatedly as faculty learned from experts, the literature, and faculty who had already developed a CURE. Our results illustrate that a community of practice structure can enhance the learning aspect of the community, helping faculty consider their implementation of inclusive, equitable, and high-impact practices as an ongoing educational process for themselves and emphasizing the importance of reflection and iteration in a DOI framework.
Microtubule acetylation is not required for HIV-1 infection or TRIM69-mediated restriction of HIV-1 infection
Authors: Lichon Drew M. , LoMascolo Natalie J. , Mounce Bryan C. , Campbell Edward M. 
Published: January 2025

ABSTRACT

Microtubule acetylation, a post-translational modification catalyzing the addition of acetyl groups to lysine residues on alpha tubulin, confers mechanical resilience to microtubules and influences intracellular cargo transport. Despite its known cellular functions, its role in viral infections remains poorly understood. The goal of this study was to determine the role of microtubule acetylation in both HIV-1 infection and TRIM69-mediated restriction. To this end, we generated CRIPSR/Cas9 vectors to disrupt alpha-tubulin acetyltransferase (αTAT1), the main enzyme responsible for microtubule acetylation. We assessed the role of acetylation in HIV-1 infectivity and the degree to which TRIM69 relies on microtubule acetylation for its ability to restrict HIV-1. We determined that microtubule acetylation is not required for HIV-1 infection and that preventing microtubule acetylation actually leads to a modest increase in HIV-1 infection. We also determined that TRIM69 can restrict a diverse range of viruses and that its restriction of HIV-1 does not rely on microtubule acetylation.

IMPORTANCE
Although microtubule acetylation is a well-studied post-translational modification in the context of cellular processes, its role during viral infections remains underexplored. Existing studies often rely on various protein and drug perturbations to indirectly examine microtubule acetylation. In this study, we directly target the enzyme responsible for microtubule acetylation to delineate its role in both HIV-1 infection and TRIM69-mediated restriction.
CD93 blockade promotes effector T-cell infiltration and facilitates adoptive cell therapy in solid tumors
Authors: Sun Yi , Yee Elliott , Fujiwara Yuki , Dickinson Kaitlyn , Guo Yujie , Sun Zhiwei , Hu Junyi , Davila Eduardo , Schulick Richard D , Zhu Yuwen 
Published: January 2025

BackgroundAdaptive cellular therapy (ACT), particularly chimeric antigen receptor (CAR)-T cell therapy, has been successful in the treatment of hemopoietic malignancies. However, poor trafficking of administered effector T cells to the tumor poses a great hurdle for this otherwise powerful therapeutic approach in solid cancers. Our previous study revealed that targeting CD93 normalizes tumor vascular functions to improve immune checkpoint blockade therapy. The objective of this study is to evaluate whether CD93 blockade improves ACT in solid cancers.MethodsMonoclonal antibodies (mAbs) against CD93 or IGFBP7 were administered in implanted mouse melanoma models to assess the effect of CD93 blockade on ACT. Different sources of effector T cells were used, including pre-activated CD8+OT-1, pmel-1 transgenic T cells, and CAR-T cells. Rip-OVA and Rip-TAG-OVA transgenic mice were used to evaluate the selective impact of CD93 blockade on effector T-cell infiltration in tumors. For mechanistic studies, vascular maturation was determined by immunofluorescent staining and flow cytometry was performed to examine tumor-infiltrating T lymphocytes. Neutralizing mAbs against adhesion molecules ICAM1 and VCAM1 were infused to assess their involvement.ResultsBlockade of the CD93 pathway increases the expression of adhesion molecules on tumor vasculature to improve effector T-cell infiltration and function. T-cell transfer and CD93 blockade synergistically improve tumor vascular maturation, as well as inhibit tumor progression. Anti-CD93 selectively promotes effector T-cell infiltration in a tumorous setting where the CD93 pathway is upregulated. In a solid mouse tumor model, blockade of the CD93 pathway improves CAR-T therapy.ConclusionsCD93 blockade normalizes tumor vasculature leading to improved effector T-cell infiltration and function in solid cancers. Our study advocates the application of CD93 blockade for ACT in solid cancers.