bluuebunny/crossref_metadata_2025_split huggingface dataset:
Integrated multiplex analysis of cell death regulators in stage II colorectal cancer suggests patients with ‘persister’ cell profiles fail to benefit from adjuvant chemotherapy
Authors: Ginty Fiona , Sanghee Cho , McDonough Elizabeth , Graf John , Shia Jinru , Lindner Andreas , Salvucci Manuela , Matveeva Anna , Kisakol Batuhan , O’Grady Tony , Burke John , McNamara Deborah , McDade Simon , Surrette Christine , Longley Daniel , Prehn Jochen 
Published: September 2023

Abstract
Apoptosis is regulated by intrinsic and extrinsic signaling orchestrated through multiple proteins that initiate or inhibit apoptosis. Inducing tumor cell apoptosis is a primary objective of chemotherapy, but to date, biomarkers of apoptosis sensitivity or resistance have shown mixed results. To understand the interplay between these proteins and whether the balance of apoptosis regulators influences chemotherapy responses and patient outcomes, we conducted multiplexed imaging of 16 proteins in the intrinsic and extrinsic apoptosis pathways at single cell resolution on resected tissue from 194 stage II colorectal cancer (CRC) patients who either received adjuvant chemotherapy (n=108) or were treated with surgery only (n=86). K-means clustering of >400,000 individual cancer cells and cell-level intensities of Apaf-1, procaspase-9, procaspase-3, XIAP, SMAC, BAX, BAK, Bcl-2, Bcl-xL, MCL-1, procaspase-8, BID, FADD, FLIP, RIP3 and cIAP1 identified distinct cell cluster profiles and demonstrated profound patient-to-patient heterogeneity. In chemotherapy-treated stage II CRC patients, those with a higher percentage of cell clusters with low procaspase-3 and high XIAP had a higher risk of recurrence. The high risk cell cluster (low-procaspase 3, high XIAP) was validated in an independent cohort of adjuvant chemotherapy-treated high-risk stage II CRC patients. To further interrogate the apoptosis sensitivity of the cell clusters, we also applied two established systems models of apoptosis initiation and execution, the BCL-2 pathway (DR_MOMP) and the caspase activation pathway (APOPTO-CELL). Here we showed that cell clusters associated with increased recurrence risk do not appear have impaired MOMP sensitivity, but downstream procaspase-3 cleavage is compromised. This represents a key characteristic of drug-tolerant ‘persister’ cells. Our study represents the most comprehensive, integrated analysis to date of apoptosis protein distribution at single-cell level in CRC tumors and identifies a subgroup of stage II patients with an apoptosis resistant, ‘persister’ cell profile who do not benefit from adjuvant chemotherapy.
A new moduli for Banach spaces, Dunkl-Williams constant and Holder continuity of radial projection
Authors: Harandi Alireza Amini , Rahimi M. , Hafshejani A. Sadeghi 
Published: September 2023

Abstract
Let $X$ be a Banach space and let $DW(X)$ denotes the Dunkl-Williams constant of $X$. In this paper, we first introduce moduli $ \gamma_{X}:[0,2] \to [0,1]$ and show that $DW(X)=\sup\limits_{0<\epsilon \leq 2}\frac{\epsilon}{\gamma_{X}(\epsilon)}$, which provides a simple formula for calculating $DW(X)$ in terms of $\gamma_{X}(\epsilon)$. Then, we compute $\gamma_{H}(\epsilon)$, where $H$ is a Hilbert space, $\gamma_{l_{\infty}-l_1}(\epsilon)$, $\gamma_{l_{2}^{\infty}}(\epsilon)$ and we conclude that $DW(l_{\infty}-l_1)=1+\sqrt{2}$. Furthermore, we obtain a generalized Dunkl-Williams inequality and we conclude that, for each $\alpha\in (0,1)$, the radial projection on $X$ is $\alpha$-H\"older continuous.
Mathematics Subject Classification (2010). 46B20.
A Lifestyle Modification Program for Secondary Prevention of Atrial Fibrillation: A Pilot Study
Authors: Ashburner Jeffrey M. , Carmichael Taylor D. , Bhattacharya Romit , Bapat Aneesh C. , Natarajan Pradeep , Atlas Steven J. , Singer Daniel E. , Thorndike Anne N. 
Published: September 2023

Abstract
Background
Lifestyle modification programs, such as cardiac rehabilitation, may reduce atrial fibrillation (AF) burden and improve quality of life (QOL), but remain unproven. The objective of this pilot study was to assess feasibility, acceptability, and preliminary effectiveness of an exercise and nutrition-based cardiac rehabilitation-like program for AF patients.
Methods
We enrolled overweight adults aged ≥ 30 years with symptomatic AF in a 12-week cardiac lifestyle group program, including 6 virtual and 6 in-person visits. All visits included discussion and education about nutrition, exercise, and behavior modification. In-person visits included supervised aerobic exercise and strength training. Outcomes at baseline and 12 weeks included feasibility of participation, acceptability, change in weight and BMI, and changes in survey-based AF burden, symptoms, and QOL.
Results
From 84 invitees, 11 (13.1%) were enrolled (mean age 64; baseline BMI 38 kg/m2); 9 (82%) completed the program. Patients attended an average of 9.7 (81%) visits (Range: 6–11). Mean weight loss was 9.1 pounds (Range: 0–16); mean BMI decrease was 1.4 kg/m2 (Range: 0-2.6). Patients found the program helpful overall: all reported making diet and exercise changes during the program. Compared to baseline, patients reported decreased AF burden (12.9 vs. 11.7, p = 0.03) and symptom (10.1 vs. 5.6, p = 0.003) scores at the conclusion of the program. Patients also reported increased QOL overall (68.9 vs. 86.4, p = 0.001)
Conclusions
Participation in a cardiac rehab-like program was feasible and acceptable for overweight patients with symptomatic AF. Results suggest preliminary effectiveness of the program for reducing AF burden and symptoms and increasing QOL.
Brain glucose induces fungicidal tolerance during fungal meningitis
Authors: Wang Linqi , Chen Lei , Tian Xiuyun , Zhang Lanyue , Wang Wenzhao , Hu Pengjie , Ma Zhongyi , Li Yeqi , Li Shibin , Shen Zhenghao , Fan Xin , Ye Leixin , weixin ke , Wu Yao , Shui Guanghou , Xiao Meng , He Guang-Jun , Yang Ying , Bai Fan , Liao Guojian , Chen Min , Lin Xiaorong , Li Chong , Fang Wenxia 
Published: September 2023

Abstract
In bacteria, antibiotic tolerance, the ability of a susceptible population to survive high doses of cidal drugs, has been shown to compromise therapeutic outcomes. In comparison, whether fungicide tolerance can be induced by host-derived factors during fungal diseases remains unproven. Here, through a systematic evaluation of metabolite-drug-fungal interactions in the leading fungal meningitis pathogen, Cryptococcus neoformans, we found that glucose, on which the brain depends for fuel, induces fungal tolerance to amphotericin B (AmB) in mouse brain tissue and patient cerebrospinal fluid via the fungal glucose repression activator Mig1. Mig1-mediated tolerance limits treatment efficacy for cryptococcal meningitis in mice and occurs through inhibiting the synthesis of ergosterol, the target of AmB, and promoting the production of inositolphosphorylceramide, which competes with AmB for ergosterol. Furthermore, AmB combined with an inhibitor of fungal-specific inositolphosphorylceramide synthase, aureobasidin A (AbA), shows a better efficacy against cryptococcal meningitis in mice than do clinically recommended therapies. Our discoveries provide evidence of host metabolite-induced fungicidal tolerance, which limits treatment efficacy for fungal meningitis.
Putative hybrids between Indian giant squirrels (Ratufa indica) and grizzled giant squirrels (Ratufa macroura): a case study from the Western Ghats, India.
Authors: G SIVA SANKARI , THAMPY DENCIN RONS , MOTI AKSHAY 
Published: September 2023

AbstractNatural hybridization, long considered insignificant, is now recognized as one of the major driving forces for the diversification of vertebrate groups. It often occurs when previously allopatric species are forced into sympatry due to anthropogenic or natural causes. The impacts of hybridization on many rare and threatened animal groups are not clearly understood. We present here a detailed account of the possible hybridization between two species of oriental giant squirrels endemic to the Indian Sub-continent; the Indian giant squirrel (Ratufa indica) and the grizzled giant squirrel (Ratufa macroura) from the Western Ghats, India. A total of eleven putative hybrids could be recorded from the Chinnar Wildlife Sanctuary in Kerala. We present here the conclusive evidence for reproductive viability among the putative hybrids. The distribution of putative hybrids in the wildlife sanctuary is recorded, and the major factors contributing to the hybridization event are discussed.
Lamina Cribrosa Changes following Pars Plana Vitrectomy and Silicone Oil Injection for Rhegmatogenous Retinal Detachment: A contralateral Eye Study
Authors: Nikkhah Homayoun , Eshtahbanati Fatemeh Foroudi , Hooshmandi Sadid , Karimi Saeed , Sazgar Amir Keyvan , Nouri Hosein , Abtahi Seyed-Hossein , Forouhari Ali 
Published: September 2023

Abstract
Purpose
To assess changes in lamina cribrosa (LC) characteristics in eyes with silicone oil (SO) due to rhegmatogenous retinal detachment (RRD).
Method
This cross-sectional contralateral eye study was conducted on 49 patients with unilateral RRD who underwent pars plana vitrectomy and SO injection. The contralateral eyes served as controls. Thickness and depth of LC of both eyes of the participants were evaluated using enhanced depth imaging technique via optical coherence tomography.
Results
The average age of the participants was 57.6 ± 10.5 years and the mean duration of post-op SO retainment was 1.7 ± 6.4 months. The mean LCD in eyes with SO tamponade was 347.6 ± 64.3 µm, whereas, in healthy eyes, it was 329.6 ± 76.7 µm (P = 0.232). On the other hand, the LC in eyes with SO tamponade was significantly thinner compared to that in healthy eyes (270.1 ± 45.1 µm and 303.2 ± 48.6 µm, respectively) (P < 0.0001). Subgroup analysis revealed that after excluding patients who were underwent anti-glaucoma medications, similar findings was resulted.
Conclusion
We showed that eyes with silicone oil exhibit a significantly thinner LC compared to healthy eyes, with no difference in the depth of the LC between the two groups.
Data-Driven Reduced-Order Modeling of Blood Flow in a Constricted Channel with Magnetic Field Effect using Deep Learning
Authors: Farooq Hamayun , Ali Amjad , Umar Muhammad , Ahmad Naveed , Kazmi Syeda Zahra 
Published: September 2023

Abstract
In an era characterized by an unprecedented influx of data across various domains, the quest for efficient, accurate, and computationally feasible modeling or reduced-order modeling (ROM) techniques has become paramount. This paper embarks on an exploration of leveraging the power of artificial neural networks (ANNs) as a data-driven approach to constructing ROM for non-Newtonian blood flows with magnetic field effects in an artery with plaque (a two-dimensional channel with uniform constriction). Several highdimensional simulations are performed in the parametric space of the Reynolds number and Hartmann number to generate the dataset for the training of the deep-learning model. The parametric space constitutes a wide range of Reynolds numbers from 1.0×102 to 1.1×103 and Hartmann numbers from 0 to 30. The snapshots of the vorticity field have been utilized to construct the proper-orthogonal decomposition (POD) to reconstruct the data-driven lowdimensional version of vorticity. In the ANN model, the past-time temporal coefficients along with the case value of the Reynolds number and Hartmann number used as the input to predict the future-time temporal coefficients following through the deep neural networks. These coefficients, in turn, are used to reconstruct the vorticity in a Galerkin fashion. The accuracy of the trained ANN model is tested by predicting the coefficients of several unseen cases, those which were not used in the training process. Results show remarkable predictive accuracy of the trained model. Thus, the ANN-based ROM (ANN-ROM) can be used as a real-time device in the field of biomechanical engineering to accurately predict the flow field and shear stresses on the artery surfaces.
Consumers’ safety of some selected ready to eat and street vended foods in Wudil, Kano state as determined by microbial content
Authors: Garba Abubakar Ibrahim 
Published: September 2023

Because of their cost, affordability, availability and simplicity to prepare, ready-to-eat foods are highly consumed in all over Nigeria, therefore critical to Nigerians’ health. This research aimed at ascertaining the consumers’ safety in terms of microbial load in ready-to-eat sell on the street Awara (Steamed Cowpea Beans Slurry) and Moin-moin (Bean pie) sold in three different vending locations retailed to the students of Aliko Dangote University of Science and Technology Wudil, Kano state Nigeria. Total of 24 samples consisting of equal quantity of Awara (Bean pie) and Moin-moin (Steamed Cowpea Bean Slurry) were collected from three different location in Wudil town and analyzed for total aerobic bacteria, fungi and Staphyococcus count. Seven different species of microorganism of health concern were identified to include Micrococcus spp, Staphylococcus aureous, Bacillus spp., Klebsiella spp., Mucor, Rhizophus stoonifer and Aspergillus flavus. The total mean of the aerobic bacteria, fungi and Staphylococcus count across all the location in all the samples were found to be withing the tolerable range with the standard set by the International Commission for Microbial Specification for Foods (ICMSF) and Food and Environmental Hygiene Standard (FEHD) as well as the South Wales Food Authority Standards for ready-to-eat foods. Higher microbial counts were founds on Moin-moin sample and this may be attributed to its high moisture content which is found to range from 64.2 to 70.0 %. The comparison of this research finding with international standard justified the safety of consumers and concludes the acceptability of these foods as safe to eat even though the presence of microorganisms such as Aspergilus spp. has been found to pose serious public health issue such as aflatoxin intoxication
CD39+MDSC Facilitates Cisplatin Resistance of Ovarian Cancer Cells by
Affecting Adenosine Synthesis
Authors: Ma Jiong , Zhou Chunxia , Zheng Xia , Chen Xuejun 
Published: September 2023

Background::
Chemoresistance is a major cause of relapse or death in ovarian cancer (OC) patients.
New evidence suggests the crucial role of myeloid-derived suppressor cells (MDSCs) in mediating chemoresistance
of cancer cells. We aimed to dissect the way MDSCs affect the cisplatin resistance phenotype of OC and
the related mechanisms.

Methods::
MDSCs were isolated from the spleen of OC mice isograft. CCK-8 and colony formation assays revealed
the effects of an MDSC-conditioned medium with dysregulated CD39 on the proliferation and cisplatin
sensitivity of OC cells. Fluorescence assay was used to reveal the effects of MDSCs with dysregulated CD39
on adenosine triphosphate (ATP) hydrolysis and adenosine (ADO) synthesis.

Results::
MDSCs with highly expressed CD39 could facilitate the proliferation and cisplatin resistance of OC
cells, while MDSCs with downregulated CD39 caused the opposite results. In addition, MDSCs with upregulated
CD39 could facilitate the hydrolysis of immunogenic ATP to immunosuppressive ADO, while the introduction
of CD39 inhibitor could repress such hydrolysis of ATP and generation of ADO, thereby abating the
proliferation and cisplatin resistance of OC cells.

Conclusion::
CD39+MDSC could promote the proliferation and cisplatin resistance of OC cells by generating
high concentrations of ADO, which indicates that targeting CD39+MDSC might be a feasible way to improve
cisplatin resistance in OC.
Diversity and Isolation of Endophytic Fungi in Panax japonicus and
Biotransformation Activity on Saponins
Authors: Li Pengfei , Ling Xiaofeng , Zhao Shujuan , Xu Lili , Wang Rufeng 
Published: September 2023

Objectives::
This study reports the diversity and community structure differences of
the endophytic fungi of Panax japonicus of different ages to obtain novel endophytic fungi with
glycoside hydrolytic activity for rare saponins production.

Methods::
This study used the high-throughput sequencing method to analyze the diversity and
community structure of endophytic fungi of P. japonicus. The endophytic fungi were processed
by traditional isolation, culture, conservation, and ITS rDNA sequence analyses. Then the total
saponins of P. japonicus were used as the substrate to evaluate the glycoside hydrolytic activity.

Results::
The composition analysis of the community structure showed that the abundance, evenness,
and diversity of endophytic fungi of nine-year-old P. japonicus were the best among all
samples. A total of 210 endophytic fungi were isolated from P. japonicus samples and further
annotated by sequencing the internal transcribed spacer. Then the biotransformation activity of
obtained strains was further examined on total saponins of P. japonicus (TSPJ), with a strain
identified as Fusarium equiseti (No.30) from 7-year-old P. japonicus showing significant glycoside
hydrolytic activity on TSPJ, including ginsenoside Ro→zinglbroside R1, pseudoginsenoside
RT1→pseudoginsenoside RP1, chikusetsusaponin IV→tarasaponin VI and chikusetsusaponin
IVa →calenduloside E.

Conclusion::
These results reveal the diversity and community structure differences of the endophytic
fungi of P. japonicus with different ages and establish a resource library of endophytic
fungi of P. japonicus. More importantly, we identified a valuable endophytic fungus with glycoside
hydrolytic activity and provided a promising convenient microbial transformation approach
to produce minor deglycosylated ginsenosides.
Exosome miR-30a-5p Regulates Glomerular Endothelial Cells' EndMT
and Angiogenesis by Modulating Notch1/VEGF Signaling Pathway
Authors: Ning Yaxian , Zhou Xiaochun , Wang Gouqin , Zhang Lili , Wang Jianqin 
Published: September 2023

Background:
Diabetic nephropathy (DN) is one of the microvascular complications of
diabetes. Endothelial-mesenchymal transition (EndMT) and endothelial damage lead to abnormal
angiogenesis in DN.

Objective:
This study aimed to investigate the role of exosome miR-30a-5p in high glucose
(HG)-induced glomerular endothelial cells (GECs) dysfunction and explore the underlying mechanisms.

Methods:
GECs were cultured in normal glucose (5.5 mM) and HG (30 mM) conditions. The recipient
GECs were transfected with exosome or miR-30a-5p mimic/inhibitor and then detected by
using CCK-8 and flow cytometry assay. Luciferase analysis was used to verify miR-30a-5p acted
on notch homolog protein 1 (Notch1). RT-qPCR and Western blot were used to detect the expression
of VE-cadherin, α-SMA, vascular endothelial growth factor (VEGF) and Notch1. In vivo, exosome
miR-30a-5p was administered to DN mice, and periodic acid-Schiff (PAS) staining, UTP
levels, and HbA1c levels were measured.

Results:
The expression of miR-30a-5p was downregulated in HG-treated GECs. Exosome
miR-30a-5p significantly promoted cell proliferation, and migration and reduced apoptosis of
GECs under HG conditions. MiR-30a-5p directly targeted the 3-UTR region of Notch1. Exosome
miR-30a-5p reduced the expression levels of Notch1 and VEGF, both at mRNA and protein levels.
Furthermore, exosome miR-30a-5p inhibited HG-induced EndMT, as evidenced by increased
VE-cadherin and reduced α-SMA. In vivo studies demonstrated that exosome miR-30a-5p reduced
serum HbA1c levels and 24-hour urine protein quantification.

Conclusion:
This study provides evidence that exosome miR-30a-5p suppresses EndMT and abnormal
angiogenesis of GECs by modulating the Notch1/VEGF signaling pathway. These findings
suggest that exosome miR-30a-5p could be a potential therapeutic strategy for the treatment
of DN.
Extracorporeal Shock Wave Therapy as Regenerative Medicine in
Knee Osteoarthritis: Pre-Experiment for Matrix Metalloproteinase-3
Evaluation
Authors: Randita Amandha Boy Timor , Wardhani Indrayuni Lukitra , Andriati Andriati , Wardani Nurul Kusuma , Philothra Petrina Theda , Subadi Imam , Melaniani Soenarnatalina 
Published: September 2023

Background:
Knee osteoarthritis (KOA) is currently treated by regenerative therapies
that aim to inhibit arthritic degeneration. Extracorporeal shock wave therapy (ESWT) is one of the
physical regenerative approaches used for KOA management. However, little is known regarding
the impact of shock wave treatment on matrix metalloproteinase-3 (MMP-3), which is one of the
enzymes mediating cartilage degradation.

Objective:
To evaluate the effect of ESWT on MMP-3 levels and pain intensity in patients with
KOA.

Methods:
Fourteen patients diagnosed with Kellgren Lawrence, grades 2 and 3 KOA were recruited
for the study. ESWT piezo shockwave was applied once a week for six weeks. MMP-3 levels
in the blood were measured pre-test, mid-test (three weeks after therapy) and post-test (one week
after the last session) by enzyme-linked immunosorbent assay (ELISA). The perceived pain was
recorded at each session by the Wong Becker Face Scale.

Results:
The median pre-test, mid-test and post-test MMP-3 levels were 19.92 ng/mL, 15.89
ng/mL and 18.82 ng/mL, respectively, and there were significant differences between the pre-test
and mid-test, and the pre-test and post-test values (p < 0.05). The pain scores also decreased significantly
over the period of intervention.

Conclusion:
MMP-3 levels decreased significantly in KOA patients after ESWT, and the decline
was most obvious after 3 weeks of therapy. Therefore, EWST should be considered as a suitable
treatment option for KOA.
The Application of Islamic Value-Based Empowerment of MSMEs to Improve the Performance of Micro Businesses in Panongan Subdistrict Tangerang
Authors: Jamaludin Nur , Muizzudin Muizzudin 
Published: September 2023

The population growth rate in Panongan, one of the subdistricts in Tangerang Regency, is 6.5%, while Tangerang Regency is 3.4% and Banten is 2.7%. This population explosion not only has the potential to cause economic, social and population problems but also provides opportunities for market growth and new economic opportunities. The purpose of this research is to describe the implementation of Islamic value-based empowerment of MSMEs in the form of the community service program called as the community engagement program run by FEBI UCA to assist 20 Micro, Small and Medium Enterprises (MSMEs) in Panongan Subdistrict. The method of this research is Participatory Action Research (PAR) emphasizing collaboration between researchers and the communities of MSMEs being studied. The research result shows that business motivation, marketing, bookkeeping, partnership and cooperation increase better than before. The increase opens wide opportunities of business and grabs more fortune.
أثر الانفاق العسكري علی معدلات البطالة في مجموعة من الدول النامیة للمدة (2005-2020) باستخدام panel data
Authors: محمد صالح زوزان 
Published: September 2023

تعد السيادة من مقومات الحياة العامة ومن مقومات الدولة الأساسية التي تهدف إلى تلبية مطالب ومتطلبات الأفراد التي يتكون منها المجتمع، لذا یتطلب غیاب الامن والاستقرار تخصیص مبالغ كبیرة أو مایسمی بالانفاق العسكري لتأمین وتوفیر مقومات الاستقرار، هذا من جهة ومن جهة أخری یعد الانفاق العسكري بمثابة مشكلة وعقبة في تحقیق التقدم للأنشطة المدنیة خاصة في البلدان النامیة والبلدان التي تتمیز بمعدل نمو حقیقي منخفض كالدول قید البحث والتي تترك هذه النفقات آاثارا سلبیة علی المتغیرات الاقتصادیة والاجتماعیة ومنها البطالة، ويطمح هذا البحث في تفسير، وتحليل أثر الانفاق العسكري علی معدلات البطالة في الدول التي تتصف بزیادة انفاقها العسكري مثل ( العراق وایران وتركیا وجمهوریة مصر العربیة). وأخذ البحث معدلات البطالة كمتغير تابع ( Dependent variable )داخل النموذج وبعض المتغيرات المستقلة (Independent variable) الذي يتوقع أن يكون لهذه المتغيرات دور في زيادة، أو انخفاض معدلات البطالة، وذلك بالاعتماد على النظرية الاقتصادية، مثل (الانفاق العسكري كنسبة من GDP، والاستثمار الاجنبي كنسبة من DGP، ونموGDP)، وأهم ما توصل الیه هذا البحث هو أن الزیادة الكبیرة للإنفاق العسكري يزاحم الميزانية الاقتصادية، مما يؤدي إلى قيود الميزانية لقطاعات أخرى مثل التعليم والصحة والإنتاجية الصناعية خاصة في الدول النامیة، ویقترح البحث في ضرورة اتجاه هذه الدول نحو معدل نمو مستقر من خلال نمو القطاعات الاقتصادیة نموا حقیقیا وتحويل مواردها الاستثمارية إلى مجالات أخرى تضمن نموها الاقتصادي.