bluuebunny/crossref_metadata_2025_split huggingface dataset:
Gas phase synthesis of the C40 nano bowl C40H10
Authors: Tuli Lotefa B. , Goettl Shane J. , Turner Andrew M. , Howlader A. Hasan , Hemberger Patrick , Wnuk Stanislaw F. , Guo Tianjian , Mebel Alexander M. , Kaiser Ralf I. 
Published: March 2023

AbstractNanobowls represent vital molecular building blocks of end-capped nanotubes and fullerenes detected in combustion systems and in deep space such as toward the planetary nebula TC-1, but their fundamental formation mechanisms have remained elusive. By merging molecular beam experiments with electronic structure calculations, we reveal a complex chain of reactions initiated through the gas-phase preparation of benzocorannulene (C24H12) via ring annulation of the corannulenyl radical (C20H9•) by vinylacetylene (C4H4) as identified isomer-selectively in situ via photoionization efficiency curves and photoion mass-selected threshold photoelectron spectra. In silico studies provided compelling evidence that the benzannulation mechanism can be expanded to pentabenzocorannulene (C40H20) followed by successive cyclodehydrogenation to the C40 nanobowl (C40H10) – a fundamental building block of buckminsterfullerene (C60). This high-temperature pathway opens up isomer-selective routes to nanobowls via resonantly stabilized free-radical intermediates and ring annulation in circumstellar envelopes of carbon stars and planetary nebulae as their descendants eventually altering our insights of the complex chemistry of carbon in our Galaxy.
Secreted protease PRSS35 suppresses hepatocellular carcinoma by disabling CXCL2-mediated neutrophil extracellular traps
Authors: Wang Ting , Zhou Yingli , Zhou Zilong , Zhang Pinggen , Yan Ronghui , Sun Linchong , Ma Wenhao , Zhang Tong , Shen Shengqi , Liu Haiying , Lu Hui , Ye Ling , Feng Junru , Chen Zhaolin , Zhong Xiuying , Wu Gao , Cai Yongping , Jia Weidong , Gao Ping , Zhang Huafeng 
Published: March 2023

AbstractHepatocytes function largely through the secretion of proteins that regulate cell proliferation, metabolism, and intercellular communications. During the progression of hepatocellular carcinoma (HCC), the hepatocyte secretome changes dynamically as both a consequence and a causative factor in tumorigenesis, although the full scope of secreted protein function in this process remains unclear. Here, we show that the secreted pseudo serine protease PRSS35 functions as a tumor suppressor in HCC. Mechanistically, we demonstrate that active PRSS35 is processed via cleavage by proprotein convertases. Active PRSS35 then suppresses protein levels of CXCL2 through targeted cleavage of tandem lysine (KK) recognition motif. Consequently, CXCL2 degradation attenuates neutrophil recruitment to tumors and formation of neutrophil extracellular traps, ultimately suppressing HCC progression. These findings expand our understanding of the hepatocyte secretome’s role in cancer development while providing a basis for the clinical translation of PRRS35 as a therapeutic target or diagnostic biomarker.
The structural flexibility of MAD1 facilitates the assembly of the Mitotic Checkpoint Complex
Authors: Chen Chu , Piano Valentina , Alex Amal , Han Simon J. Y. , Huis in ’t Veld Pim J. , Roy Babhrubahan , Fergle Daniel , Musacchio Andrea , Joglekar Ajit P. 
Published: March 2023

AbstractThe spindle assembly checkpoint (SAC) safeguards the genome during cell division by generating an effector molecule known as the Mitotic Checkpoint Complex (MCC). The MCC comprises two subcomplexes: BUBR1:BUB3 and CDC20:MAD2, and the formation of CDC20:MAD2 is the rate-limiting step during MCC assembly. Recent studies show that the rate of CDC20:MAD2 formation is significantly accelerated by the cooperative binding of CDC20 to the SAC proteins MAD1 and BUB1. However, the molecular basis for this acceleration is not fully understood. Here, we demonstrate that the structural flexibility of MAD1 at a conserved hinge near the C-terminus is essential for catalytic MCC assembly. This MAD1 hinge enables the MAD1:MAD2 complex to assume a folded conformation in vivo. Importantly, truncating the hinge reduces the rate of MCC assembly in vitro and SAC signaling in vivo. Conversely, mutations that preserve hinge flexibility retain SAC signaling, indicating that the structural flexibility of the hinge, rather than a specific amino acid sequence, is important for SAC signaling. We summarize these observations as the ‘knitting model’ that explains how the folded conformation of MAD1:MAD2 promotes CDC20:MAD2 assembly.
Mesoporous Pt@Pt-skin Pt3Ni core-shell framework nanowire electrocatalyst for efficient oxygen reduction
Authors: Jin Hui , Xu Zhewei , Hu Zhi-Yi , Yin Zhiwen , Wang Zhao , Deng Zhao , Wei Ping , Feng Shihao , Dong Shunhong , Liu Jinfeng , Luo Sicheng , Qiu Zhaodong , Zhou Liang , Mai Liqiang , Su Bao-Lian , Zhao Dongyuan , Liu Yong 
Published: March 2023

AbstractThe design of Pt-based nanoarchitectures with controllable compositions and morphologies is necessary to enhance their electrocatalytic activity. Herein, we report a rational design and synthesis of anisotropic mesoporous Pt@Pt-skin Pt3Ni core-shell framework nanowires for high-efficient electrocatalysis. The catalyst has a uniform core-shell structure with an ultrathin atomic-jagged Pt nanowire core and a mesoporous Pt-skin Pt3Ni framework shell, possessing high electrocatalytic activity, stability and Pt utilisation efficiency. For the oxygen reduction reaction, the anisotropic mesoporous Pt@Pt-skin Pt3Ni core-shell framework nanowires demonstrated exceptional mass and specific activities of 6.69 A/mgpt and 8.42 mA/cm2 (at 0.9 V versus reversible hydrogen electrode), and the catalyst exhibited high stability with negligible activity decay after 50,000 cycles. The mesoporous Pt@Pt-skin Pt3Ni core-shell framework nanowire configuration combines the advantages of three-dimensional open mesopore molecular accessibility and compressive Pt-skin surface strains, which results in more catalytically active sites and weakened chemisorption of oxygenated species, thus boosting its catalytic activity and stability towards electrocatalysis.
Evaluation of the immunization effectiveness of bOPV booster immunization and IPV revaccination
Authors: Yu-ping Zhao , Jing Li , Teng Huang , Zhi-fang Ying , Ting Zhao , Yan-chun Che , Zhi-mei Zhao , Yu-ting Fu , Jun-hui Tao , Qing-hai Yang , Ding-kai Wei , Guo-liang Li , Xiao-lei Yang , Li Yi , Hong-bo Chen , Jian-feng Wang , Rui-ju Jiang , Lei Yu , Wei Cai , Wei Yang , Ming-xue Xie , Qiong-zhou Yin , Jing Pu , Li Shi , Chao Hong , Yan Deng , Lu-kui Cai , Jian Zhou , Yu Wen , Hong-sen Li , Wei Huang , Zhao-jun Mo , Chang-gui Li , Qi-han Li , Jing-si Yang 
Published: March 2023

AbstractTo provide a basis for further optimization of the polio sequential immunization schedule, this study evaluated the effectiveness of booster immunization with one dose of bivalent oral poliovirus vaccine (bOPV) at 48 months of age after different primary polio immunization schedules. At 48 months of age, one dose of bOPV was administered, and their poliovirus types 1–3 (PV1, PV2, and PV3, respectively)-specific neutralizing antibody levels were determined. Participants found to be negative for any type of PV-specific neutralizing antibody at 24, 36, or 48 months of age were re-vaccinated with inactivated polio vaccine (IPV). The 439 subjects who received a bOPV booster immunization at the age of 48 months had lower PV2-specific antibody levels compared with those who received IPV. One dose of IPV during basic polio immunization induced the lowest PV2-specific antibody levels. On the basis of our findings, to ensure that no less than 70% of the vaccinated have protection efficiency, we recommend the following: if basic immunization was conducted with 1IPV + 2bOPV (especially Sabin strain-based IPV), a booster immunization with IPV is recommended at 36 months of age, whereas if basic immunization was conducted with 2IPV + 1bOPV, a booster immunization with IPV is recommended at 48 months of age. A sequential immunization schedule of 2IPV + 1bOPV + 1IPV can not only maintain high levels of antibody against PV1 and PV3 but also increases immunity to PV2 and induces early intestinal mucosal immunity, with relatively good safety. Thus, this may be the best sequential immunization schedule for polio in countries or regions at high risk for polio.
Agrimonia: a dataset on livestock, meteorology and air quality in the Lombardy region, Italy
Authors: Fassò Alessandro , Rodeschini Jacopo , Fusta Moro Alessandro , Shaboviq Qendrim , Maranzano Paolo , Cameletti Michela , Finazzi Francesco , Golini Natalia , Ignaccolo Rosaria , Otto Philipp 
Published: March 2023

AbstractThe air in the Lombardy region, Italy, is one of the most polluted in Europe because of limited air circulation and high emission levels. There is a large scientific consensus that the agricultural sector has a significant impact on air quality. To support studies quantifying the role of the agricultural and livestock sectors on the Lombardy air quality, this paper presents a harmonised dataset containing daily values of air quality, weather, emissions, livestock, and land and soil use in the years 2016–2021, for the Lombardy region. The daily scale is obtained by averaging hourly data and interpolating other variables. In fact, the pollutant data come from the European Environmental Agency and the Lombardy Regional Environment Protection Agency, weather and emissions data from the European Copernicus programme, livestock data from the Italian zootechnical registry, and land and soil use data from the CORINE Land Cover project. The resulting dataset is designed to be used as is by those using air quality data for research.
Urinary exosomal miRNA-663a shows variable expression in diabetic kidney disease patients with or without proteinuria
Authors: Sinha Nisha , Puri Veena , Kumar Vivek , Nada Ritambhra , Rastogi Ashu , Jha Vivekanand , Puri Sanjeev 
Published: March 2023

AbstractHeterogeneity in the Diabetic Kidney Disease (DKD) diagnosis makes its rational therapeutics challenging. Although albuminuria characterizes DKD, reports also indicate its prevalence among non-proteinuric. Recent understanding of disease progression has thus inclined the focus on proximal tubular cell damage besides the glomeruli. A non-invasive approach exploiting exosomal miRNA derived from human kidney proximal tubular cell line was, hence, targeted. Upon miRNA profiling, three miRNAs, namely, hsa-miR-155-5p, hsa-miR-28-3p, and hsa-miR-425-5p were found to be significantly upregulated, while hsa-miR-663a was downregulated under diabetic conditions. Among these, hsa-miR-663a downregulation was more pronounced in non-proteinuric than proteinuric DKD subjects and was thus selected for the bioinformatics study. Ingenuity Pathway Analysis (IPA) narrowed on to IL-8 signaling and inflammatory response as the most enriched ‘canonical pathway’ and ‘disease pathway’ respectively, during DKD. Further, the putative gene network generated from these enriched pathways revealed experimentally induced diabetes, renal tubular injury, and decreased levels of albumin as part of mapping under ‘disease and function’. Genes target predictions and annotations by IPA reiterated miR-663a’s role in the pathogenesis of DKD following tubular injury. Overall, the observations might offer an indirect reflection of the underlying mechanism between patients who develop proteinuria and non-proteinuria.
Tranexamic acid for the prevention of postpartum haemorrhage: the TAPPH-1 pilot randomized trial and lessons learned for trials in Canadian obstetrics
Authors: Alam Asim Q. , Barrett Jon , Callum Jeannie , Kaustov Lilia , Au Shelly , Fleet Andrew , Kiss Alex , Choi Stephen 
Published: March 2023

AbstractPostpartum haemorrhage (PPH) is a leading cause of maternal morbidity and mortality. While tranexamic acid (TXA) reduces bleeding and transfusion requirements in established PPH, we sought to determine the feasibility of conducting a fully powered trial assessing the effect of prophylactic tranexamic acid, prior to PPH onset, in a Canadian Obstetric setting. With institutional and Health Canada approval, consenting, eligible parturients (singleton, > 32 weeks gestation, vaginal or caesarian delivery) were randomly assigned to receive TXA (1 g intravenously) or placebo (0.9% saline) prior to delivery. Participants, investigators, data collectors/adjudicators, and analysis was blinded. The primary outcome was administration of study intervention to > 85% of randomized individuals. Secondary outcomes included recruitment rate (feasibility) and safety outcomes. Over 8 months, 611 were approached, 35 consented, and 27 randomized (14 TXA, 13 placebo). 89% of randomized participants received the assigned intervention. Recruitment fell below feasibility (23% target). No serious adverse outcomes occurred. Our pilot trial in a Canadian Obstetric setting was unable to demonstrate feasibility to conduct a large, multicentre trial to examine prophylactic use of tranexamic for PPH secondary to the complex regulatory requirements associated with a trial for an off-label, but commonly utilized intervention. These challenges should inform stakeholders on the resources and challenges of conducting future trials using off-label interventions.Trial registration: www.clinicaltrials.gov, NCT03069859 (03/03/2017).
Biomimetic ion substituted and Co-substituted hydroxyapatite nanoparticle synthesis using Serratia Marcescens
Authors: Paramasivan Mareeswari , Sampath Kumar T. S. , Kanniyappan Hemalatha , Muthuvijayan Vignesh , Chandra T. S. 
Published: March 2023

AbstractBiomimicry is becoming deep-rooted as part of bioceramics owing to its numerous functional advantages. Naturally occurring hydroxyapatite (HA) apart from primary nano structures are also characterised by various ionic substitutions. The ease of accommodating such key elements into the HA lattice is known to enhance bone healing properties of bioceramics. In this work, hydroxyapatite synthesized via biomimetic approach was substituted with individual as well as multiple cations for potential applications in bone repair. Ion substitutions of Sr, Mg and Zn was carried out on HA for the first time by using Serratia grown in a defined biomineralization medium. The individual ions of varying concentration substituted in Serratia HA (SHA) (Sr SHA, Mg SHA and Zn SHA) were analysed for crystallinity, functional groups, morphology and crystal size. All three showed decreased crystallinity, phase purity, large agglomerated aggregates and needle-shaped morphologies. Fourier transform infrared spectroscopy (FTIR) spectra indicated increased carbonate content of 5.8% resembling that of natural bone. Additionally, the reduced O–H intensities clearly portrayed disruption of HA lattice and subsequent ion-substitution. The novelty of this study lies primarily in investigating the co-substitution of a combination of 1% Sr, Zn and Mg in SHA and establishing the associated change in bone parameters. Scanning electron microscope (SEM) and transmission electron microscope (TEM) images clearly illustrated uniform nano-sized agglomerates of average dimensions of 20–50 nm length and 8–15 nm width for Sr SHA; 10–40 nm length and 8–10 nm width for both Zn SHA and Mg SHA and 40–70 nm length and 4–10 nm width in the case of 1% Sr, Zn, Mg SHA. In both individual as well as co-substitutions, significant peak shifts were not observed possibly due to the lower concentrations. However, cell volumes increased in both cases due to presence of Sr2+ validating its dominant integration into the SHA lattice. Rich trace ion deposition was presented by energy dispersive X-ray spectroscopy (EDS) and quantified using inductively coupled plasma optical emission spectrometer (ICP-OES). In vitro cytotoxicity studies in three cell lines viz. NIH/3T3 fibroblast cells, MG-63 osteosarcoma cells and RAW 264.7 macrophages showed more than 90% cell viability proving the biocompatible nature of 1% Sr, Zn and Mg in SHA. Microbial biomineralization by Serratia produced nanocrystals of HA that mimicked “bone-like apatite” as evidenced by pure phase, carbonated groups, reduced crystallinity, nano agglomerates, variations in cell parameters, rich ion deposition and non-toxic nature. Therefore ion-substituted and co-substituted biomineralized nano SHA appears to be a suitable candidate for applications in biomedicine addressing bone injuries and aiding regeneration as a result of its characteristics close to that of the human bone.
Bioinformatics analysis of rheumatoid arthritis tissues identifies genes and potential drugs that are expressed specifically
Authors: He Qingshan , Ding Hanmeng 
Published: March 2023

AbstractStudies have implicated necroptosis mechanisms in orthopaedic-related diseases, since necroptosis is a unique regulatory cell death pattern. However, the role of Necroptosis-related genes in rheumatoid arthritis (RA) has not been well described. We downloaded RA-related data information and Necroptosis-related genes from the Gene Expression Omnibus (GEO), Kyoto Gene and Genome Encyclopedia (KEGG) database, and Genome Enrichment Analysis (GSEA), respectively. We identified 113 genes associated with RA-related necroptosis, which was closely associated with the cytokine-mediated signaling pathway, necroptosis and programmed necrosis. Subsequently, FAS, MAPK8 and TNFSF10 were identified as key genes among 48 Necroptosis-associated differential genes by three machine learning algorithms (LASSO, RF and SVM-RFE), and the key genes had good diagnostic power in distinguishing RA patients from healthy controls. According to functional enrichment analysis, these genes may regulate multiple pathways, such as B-cell receptor signaling, T-cell receptor signaling pathways, chemokine signaling pathways and cytokine-cytokine receptor interactions, and play corresponding roles in RA. Furthermore, we predicted 48 targeted drugs against key genes and 31 chemical structural formulae based on targeted drug prediction. Moreover, key genes were associated with complex regulatory relationships in the ceRNA network. According to CIBERSORT analysis, FAS, MAPK8 and TNFSF10 may be associated with changes in the immune microenvironment of RA patients. Our study developed a diagnostic validity and provided insight to the mechanisms of RA. Further studies will be required to test its diagnostic value for RA before it can be implemented in clinical practice.
Lightweight saliency detection method for real-time localization of livestock meat bones
Authors: Xu Tao , Zhao Weishuo , Cai Lei , Shi Xiaoli , Wang Xinfa 
Published: March 2023

AbstractExisting salient object detection networks are large, have many parameters, are bulky and take up a lot of computational resources. Seriously hinder its application and promotion in boning robot. To solve this problem, this paper proposes a lightweight saliency detection algorithm for real-time localization of livestock meat bones. First, a lightweight feature extraction network based on multi-scale attention is constructed in the encoding stage. To ensure that more adequate salient object features are extracted with fewer parameters. Second, the fusion of jump connections is introduced in the decoding phase. Used to capture fine-grained semantics and coarse-grained semantics at full scale. Finally, we added a residual refinement module at the end of the backbone network. For optimizing salient target regions and boundaries. Experimental results on both publicly available datasets and self-made Pig leg X-ray (PLX) datasets show that. The proposed method is capable of ensuring first-class detection accuracy with 40 times less parameters than the conventional model. In the most challenging SOD dataset. The proposed algorithm in this paper achieves a value of Fωβ of 0.699. And the segmentation of livestock bones can be effectively performed on the homemade PLX dataset. Our model has a detection speed of 5fps on industrial control equipment.
A comprehensive quantitative bottom-up analysis of fiber-reinforced recycled-aggregate concrete behavior
Authors: Hosseinzadeh Maedeh , Dehestani Mehdi , Samadvand Hojjat 
Published: March 2023

AbstractThis study provides a more profound understanding of the influence of the phases of fiber-reinforced recycled-aggregate concrete (FRRAC), on its elastic properties, in particular Young’s modulus and Poisson’s ratio. Multi-scale modeling analyses of mortar and FRRAC were conducted to assess the effect of variations in the fiber content, fiber elastic modulus, RA content, and water-to-cement ratio (w/c) on the elastic properties at each scale. Thus, the analytic Mori–Tanaka (MT) homogenization algorithm developed in Python programming language and the three-dimensional finite element (FE) homogenization scheme were applied to evaluate the elastic properties of FRRAC. As such, different fiber types including steel, basalt, glass, and propylene, at a volume fraction range of 0–2%, along with the variations in fiber elastic modulus, and different RA replacement levels ranging from 0 to 100% were incorporated in the modeling process at different w/c ratio. Based on the results, the Poisson’s ratio of steel FRRAC in the MT approach surges with increasing fiber content. Furthermore, the elastic modulus of FRRAC is highly susceptible to an increase in Young’s modulus of polypropylene fiber, among other fiber types. The elastic modulus of concrete experiences a sharp decrease with increasing w/c for all fiber types in both FE and MT approaches.
Phase behavior of single and multi-component liquid hydrocarbons in real reservoir rocks
Authors: Al-Kindi Ilyas , Babadagli Tayfun 
Published: March 2023

AbstractPhase-alteration phenomenon has a considerable influence on the dynamics and distribution of fluids in porous media. One of the major factors affecting the phase behaviour of fluids in reservoirs is the capillarity effect, which becomes unavoidably significant as the media becomes tighter (confinement effect) and contains more pores at nano sizes. Comprehending the nature of vaporization and condensation of hydrocarbon in such confined media is important for accurate modelling of two-phase envelopes and thereby the performance of energy production from hydrocarbon reservoirs. This paper studies the vaporization of single- and multicomponent hydrocarbons in different types of rocks (namely sandstones, limestones, tight sandstones, and shales). The vaporization temperatures were measured experimentally in each rock type and compared with boiling points measured at bulk conditions to investigate the deviation between the phase-change temperatures in capillary media and bulk values. The deviation between the measured vaporization temperatures and the bulk measurements ranged from 4.4% (1.6% in Kelvin unit) to 19.7% (5.2% in Kelvin unit) with single-component solvents and 1.4% (0.4% in Kelvin unit) to 27.6% (5.3% in Kelvin unit) with the hydrocarbon mixtures. The vaporization temperatures, obtained from the experiments, were also compared with the computed two-phase envelopes, calculated by the classical Peng-Robinson Equation of State. The deviation percentages of measured vaporization temperatures from the computed values were at least 4.4% (1.6% in Kelvin unit) with single-component solvents and 2.1% (0.7% in Kelvin unit) with the hydrocarbon mixtures.