bluuebunny/crossref_metadata_2025_split huggingface dataset:
Differences in the intraspecies copy number variation of Arabidopsis thaliana conserved and nonconserved miRNA genes
Authors: Samelak-Czajka Anna , Wojciechowski Pawel , Marszalek-Zenczak Malgorzata , Figlerowicz Marek , Zmienko Agnieszka 
Published: April 2023

AbstractMicroRNAs (miRNAs) regulate gene expression by RNA interference mechanism. In plants, miRNA genes (MIRs) which are grouped into conserved families, i.e. they are present among the different plant taxa, are involved in the regulation of many developmental and physiological processes. The roles of the nonconserved MIRs—which are MIRs restricted to one plant family, genus, or even species—are less recognized; however, many of them participate in the responses to biotic and abiotic stresses. Both over- and underproduction of miRNAs may influence various biological processes. Consequently, maintaining intracellular miRNA homeostasis seems to be crucial for the organism. Deletions and duplications in the genomic sequence may alter gene dosage and/or activity. We evaluated the extent of copy number variations (CNVs) among Arabidopsis thaliana (Arabidopsis) MIRs in over 1000 natural accessions, using population-based analysis of the short-read sequencing data. We showed that the conserved MIRs were unlikely to display CNVs and their deletions were extremely rare, whereas nonconserved MIRs presented moderate variation. Transposon-derived MIRs displayed exceptionally high diversity. Conversely, MIRs involved in the epigenetic control of transposons reactivated during development were mostly invariable. MIR overlap with the protein-coding genes also limited their variability. At the expression level, a higher rate of nonvariable, nonconserved miRNAs was detectable in Col-0 leaves, inflorescence, and siliques compared to nonconserved variable miRNAs, although the expression of both groups was much lower than that of the conserved MIRs. Our data indicate that CNV rate of Arabidopsis MIRs is related with their age, function, and genomic localization.
A vision of 14 T MR for fundamental and clinical science
Authors: Bates Steve , Dumoulin Serge O. , Folkers Paul J. M. , Formisano Elia , Goebel Rainer , Haghnejad Aidin , Helmich Rick C. , Klomp Dennis , van der Kolk Anja G. , Li Yi , Nederveen Aart , Norris David G. , Petridou Natalia , Roell Stefan , Scheenen Tom W. J. , Schoonheim Menno M. , Voogt Ingmar , Webb Andrew 
Published: April 2023

Abstract
Objective
We outline our vision for a 14 Tesla MR system. This comprises a novel whole-body magnet design utilizing high temperature superconductor; a console and associated electronic equipment; an optimized radiofrequency coil setup for proton measurement in the brain, which also has a local shim capability; and a high-performance gradient set.

Research fields
The 14 Tesla system can be considered a ‘mesocope’: a device capable of measuring on biologically relevant scales. In neuroscience the increased spatial resolution will anatomically resolve all layers of the cortex, cerebellum, subcortical structures, and inner nuclei. Spectroscopic imaging will simultaneously measure excitatory and inhibitory activity, characterizing the excitation/inhibition balance of neural circuits. In medical research (including brain disorders) we will visualize fine-grained patterns of structural abnormalities and relate these changes to functional and molecular changes. The significantly increased spectral resolution will make it possible to detect (dynamic changes in) individual metabolites associated with pathological pathways including molecular interactions and dynamic disease processes.

Conclusions
The 14 Tesla system will offer new perspectives in neuroscience and fundamental research. We anticipate that this initiative will usher in a new era of ultra-high-field MR.
Progradation of a shallow carbonate platform developed on a fault-block in the Western Tethys (lower Aptian, Sierra de Bedmar-Jódar, Prebetic of Jaén, Spain)
Authors: Martínez-Rodríguez Rafael , Nieto Luis M. , Castro José M. , de Gea Ginés A. , Ruiz-Ortiz Pedro A. , Molina José M. , Skelton Peter W. 
Published: April 2023

AbstractThe Middle Member of the Llopis Fm in the Sierra de Bedmar-Jódar Unit of the Prebetic Zone of Jaén (southern Spain) was deposited on a shallow-marine platform of the Southern Iberian Continental Margin during the earliest Aptian. Detailed field logging of nine stratigraphic sections and facies mapping have allowed seven lithofacies associations (L1–L6) to be distinguished, one siliciclastic (L1) and five carbonate facies (L2–6). The succession is composed of eight consecutive elemental sequences of lithofacies associations L1–L6. Each elemental sequence is interpreted as representing one episode of shallowing-upwards carbonate deposition in a very shallow platform-lagoon that was bounded shoreward by clastic/ooid bars and passed seaward either to stromatoporoid bioconstructions (bioherms and biostromes) or rudist biostromes. The successive elemental sequences show north-eastward progradational geometries. Three phases of platform development are identified: (1) installation of the shallow platform; (2) development of a lagoon bounded by a stromatoporoid barrier and (3) development of an Urgonian-type platform dominated by rudists. During the early Aptian, the Bedmar-Jódar platform was partially isolated from the rest of the Prebetic platform and showed overall progradation towards the NE, in contrast to the general south-eastward progradational trend of the Prebetic platform. Sedimentation was controlled by rift-generated extensional tectonics that resulted in tilting of the platform block, causing the deviation of progradation from the general trends of the Prebetic Platform. In addition, climatic influence is inferred from the presence of siliciclastic sediments derived from weathering of the hinterland, which restricted the carbonate factory.
Residential building stock in Serbia: classification and vulnerability for seismic risk studies
Authors: Blagojević Nikola , Brzev Svetlana , Petrović Milica , Borozan Jovana , Bulajić Borko , Marinković Marko , Hadzima-Nyarko Marijana , Koković Veljko , Stojadinović Božidar 
Published: April 2023

AbstractRegional seismic risk assessment is necessary for designing effective seismic risk mitigation measures. In general, such risk assessment studies consist of three components: hazard, vulnerability, and exposure modelling. This paper lays the foundations for regional seismic risk assessment of the residential building stock in Serbia and addresses each of the three seismic risk assessment components, either by reviewing the existing or proposing novel models. First, a review of seismic hazard models and seismic design codes used in Serbia in the past 70 years was presented. Next, an overview of Serbia’s population metrics and historical development of Serbian’s residential building stock was presented to provide the context for the exposure model. Furthermore, the paper proposed a novel building classification for Serbia's residential building stock, which is based on the existing building taxonomies, but it has been adapted to account for the local building characteristics. Building damage patterns reported in past earthquakes in Serbia and neighbouring countries were reviewed as a basis for damage classification pertaining to building typologies included in the proposed classification. Finally, the results of a preliminary vulnerability model were presented in the form of expert-based fragility functions derived for buildings typical of Serbia's residential building stock.
Temporal trend evaluation in monitoring programs with high spatial resolution and low temporal resolution using geographically weighted regression models
Authors: von Brömssen Claudia , Fölster Jens , Eklöf Karin 
Published: April 2023

AbstractData from monitoring programs with high spatial resolution but low temporal resolution are often overlooked when assessing temporal trends, as the data structure does not permit the use of established trend analysis methods. However, the data include uniquely detailed information about geographically differentiated temporal trends driven by large-scale influences, such as climate or airborne deposition. In this study, we used geographically weighted regression models, extended with a temporal component, to evaluate linear and nonlinear trends in environmental monitoring data. To improve the results, we tested approaches for station-wise pre-processing of data and for validation of the resulting models. To illustrate the method, we used data on changes in total organic carbon (TOC) obtained in a monitoring program of around 4800 Swedish lakes observed once every 6 years between 2008 and 2021. On applying the methods developed here, we identified nonlinear changes in TOC from consistent negative trends over most of Sweden around 2010 to positive trends during later years in parts of the country.
Air pollution modeling to support strategic environmental assessment: case study—National Emission Reduction Plan for coal-fired thermal power plants in Serbia
Authors: Josimović Boško , Todorović Dušan , Jovović Aleksandar , Manić Božidar 
Published: April 2023

AbstractThe paper presents a specific method of environmental impact assessment applied in Strategic Environmental Assessment (SEA) for the National Emission Reduction Plan (NERP) in the Republic of Serbia, based on air quality. The specificity of the approach is in the application of a semiquantitative method of multicriteria evaluation based on air dispersion modeling and the integration of SEA goals, indicators and criteria for assessing the impact of the NERP on the quality of air and other environmental elements in this method. When predicting changes in air quality for the planning horizon to 2028, the physical, geographical and climatic characteristics of the area were taken into account, as well as technical measures to reduce SO2 emissions, since this was the dominant pollutant from the Serbian coal-fired power plants studied by the NERP. Air pollution modeling was carried out using the AERMOD software package based on the data collected, and the quantitative results obtained were used in a multicriteria evaluation as part of the SEA. The results of the research indicated the importance of applying this approach in order to significantly increase objectivity in the SEA process, since it is an important element of decision making at the strategic level. In addition, a comparative presentation of the modeling results before and after application of the NERP was an important part of the SEA process, and it provided a clear insight into expected changes in the air quality. This is a key argument for making appropriate policy decisions on spatial, energy, environmental and socio-economic development in the Republic of Serbia, which, like other developing countries, is sluggishly following global trends in energy transition.
Nitrogen translocation by Highland cattle grazing in Alnus viridis-encroached pastures
Authors: Svensk Mia , Pittarello Marco , Mariotte Pierre , Nota Ginevra , Schneider Manuel K. , Frund David , Dubois Sébastien , Allan Eric , Probo Massimiliano 
Published: April 2023

AbstractDuring the last decades, Alnus viridis has expanded over former montane pastures and meadows, due to land use and abandonment. This nitrogen-fixing woody species has triggered negative agro-environmental impacts, such as nitrogen (N) leaching, soil acidification and a reduced biodiversity. The aim of this study was to estimate the N translocation from A. viridis-encroached areas to adjacent open pastures by Highland cattle. In 2019 and 2020, Highland cattle herds equipped with GPS collars were placed in four A. viridis-encroached paddocks across Italy and Switzerland. The N content was measured in A. viridis leaves, herbaceous vegetation, and cattle dung pats, which were collected throughout the grazing season. Using GPS locations and collar activity sensors, livestock activity phases were discriminated. The N ingested by cattle was estimated through the N content of herbaceous vegetation and A. viridis leaves of vegetation patches visited by cattle during 24 h before dung sampling (N24H). The N content of herbaceous vegetation significantly increased with increasing A. viridis cover. The average N content in dung pats (31.2 ± 3.4 g.kg−1 DM) was higher than average values from literature on grazing cattle. Moreover, it was positively related to the N24H. Most of this N (29.5 ± 10.3 kg ha−1 yr−1) was translocated towards resting areas, which generally occurred on flat open pastures. Our results highlight the potential of Highland cattle to effectively translocate part of the ingested N from A. viridis-encroached towards targeted open areas, thus bringing new perspective for forage yield and quality improvement in the long-term.
Crisscrossing scapes in the global flow of elite mainland Chinese students
Authors: Woo Etienne , Wang Ling 
Published: April 2023

AbstractThis paper applies Appadurai’s notion of scapes in globalisation to study international student mobility. Thirty mainland Chinese students were interviewed; the majority of whom studied at prestigious institutions in the West before enrolling in their current PhD programmes at a research-intensive university in Hong Kong (HK) in the immediate aftermath of HK’s large-scale social protests and amidst the Covid-19 pandemic. We seek to understand why these students relocated to HK to further their studies given these turbulent circumstances and how their mainlander identity and sojourns in the West influence their perceptions of HK’s social movements from the perspectives of ethnoscape and ideoscape, respectively. Our findings reveal that HK represented the ‘best’ compromise for our participants, mitigating their nostalgia for home (i.e. mainland China) whilst offering a superior education to the Chinese mainland. Most participants perceived HK as a nationalistic ideoscape, wherein HK people’s pursuit of autonomy is subordinated to the putative Chinese national interests. Moreover, ethnoscape and ideoscape dynamics were found to crisscross other scapes. Generous scholarships (i.e. financescape) provided additional incentives driving student relocations. The persistent consumption of Chinese social media (techno-mediascape) was found to have resulted in worldview conformity between our participants and the Chinese state.
DESMILS: a decision support approach for multi-item lot sizing using interactive multiobjective optimization
Authors: Kania Adhe , Afsar Bekir , Miettinen Kaisa , Sipilä Juha 
Published: April 2023

AbstractWe propose a decision support approach, called DESMILS, to solve multi-item lot sizing problems with a large number of items by using single-item multiobjective lot sizing models. This approach for making lot sizing decisions considers multiple conflicting objective functions and incorporates a decision maker’s preferences to find the most preferred Pareto optimal solutions. DESMILS applies clustering, and items in one cluster are treated utilizing preferences that the decision maker has provided for a representative item of the cluster. Thus, the decision maker provides preferences to solve the single-item lot sizing problem for few items only and not for every item. The lot sizes are obtained by solving a multiobjective optimization problem with an interactive method, which iteratively incorporates preference information and supports the decision maker in learning about the trade-offs involved. As a proof of concept to demonstrate the behavior of DESMILS, we solve a multi-item lot sizing problem of a manufacturing company utilizing their real data. We describe how the supply chain manager as the decision maker found Pareto optimal lot sizes for 94 items by solving the single-item multiobjective lot sizing problem for only ten representative items. He found the solutions acceptable and the solution process convenient saving a significant amount of his time.
Antibacterial and pH-sensitive methacrylate poly-L-Arginine/poly (β-amino ester) polymer for soft tissue engineering
Authors: Heydari Parisa , Varshosaz Jaleh , Kharaziha Mahshid , Javanmard Shaghayegh Haghjooy 
Published: April 2023

AbstractDuring the last decade, pH-sensitive biomaterials containing antibacterial agents have grown exponentially in soft tissue engineering. The aim of this study is to synthesize a biodegradable pH sensitive and antibacterial hydrogel with adjustable mechanical and physical properties for soft tissue engineering. This biodegradable copolymer hydrogel was made of Poly-L-Arginine methacrylate (Poly-L-ArgMA) and different poly (β- amino ester) (PβAE) polymers. PβAE was prepared with four different diacrylate/diamine monomers including; 1.1:1 (PβAE1), 1.5:1 (PβAE1.5), 2:1 (PβAE2), and 3:1 (PβAE3), which was UV cross-linked using dimethoxy phenyl-acetophenone agent. These PβAE were then used for preparation of Poly-L-ArgMA/PβAE polymers and revealed a tunable swelling ratio, depending on the pH conditions. Noticeably, the swelling ratio increased by 1.5 times when the pH decreased from 7.4 to 5.6 in the Poly-L-ArgMA/PβAE1.5 sample. Also, the controllable degradation rate and different mechanical properties were obtained, depending on the PβAE monomer ratio. Noticeably, the tensile strength of the PβAE hydrogel increased from 0.10 ± 0.04 MPa to 2.42 ± 0.3 MPa, when the acrylate/diamine monomer molar ratio increased from 1.1:1 to 3:1. In addition, Poly-L-ArgMA/PβAE samples significantly improved L929 cell viability, attachment and proliferation. Poly-L-ArgMA also enhanced the antibacterial activities of PβAE against both Escherichia coli (~5.1 times) and Staphylococcus aureus (~2.7 times). In summary, the antibacterial and pH-sensitive Poly-L-ArgMA/PβAE1.5 with suitable mechanical, degradation and biological properties could be an appropriate candidate for soft tissue engineering, specifically wound healing applications.
Graphical Abstract
Facilitated Subcutaneous Immunoglobulin Treatment in Patients with Immunodeficiencies: the FIGARO Study
Authors: Borte Michael , Hanitsch Leif G. , Mahlaoui Nizar , Fasshauer Maria , Huscher Dörte , Speletas Matthaios , Dimou Maria , Kamieniak Marta , Hermann Corinna , Pittrow David , Milito Cinzia 
Published: April 2023

Abstract

Purpose
The FIGARO study aims to provide insights on real-world utilization and tolerability of facilitated subcutaneous immunoglobulin (fSCIG) for primary immunodeficiency disease (PID) or secondary immunodeficiency disease (SID).

Methods
This prospective, multicenter, observational study, evaluated medical records, charts, and diaries of patients who had received at least 1 fSCIG infusion for PID or SID. Data were analyzed by cohort (PID, SID) and age groups (pediatric [< 18 years], adult [18–64 years], older adult [≥ 65 years]). Patients were followed up to 36 months.

Results
The study enrolled 156 patients: 15 pediatric, 120 adult, 21 older-adult. Twelve-month follow-up data were available for 128 patients. fSCIG was mainly prescribed for PID among patients aged < 65 years and for SID among older adults. At inclusion, 75.6% received their fSCIG infusion at home, and 78.7% self-administered. Adults were more likely to receive their initial infusion at home and self-administer (81.7% and 86.6%, respectively) than pediatric patients (53.3% each) and older adults (57.1% and 52.4%, respectively). At 12 months, the proportion of patients infusing at home and self-administering increased to 85.8% and 88.2%. Regardless of age, most patients self-administered the full fSCIG dose at home every 3–4 weeks and required a single infusion site. The tolerability profile was consistent with previous pivotal trials. Acute severe bacterial infections occurred in 0%–9.1% of patients during follow-up visits (full cohort).

Conclusions
FIGARO confirms the feasibility, tolerability, and good infection control of fSCIG in PID and SID patients across the age spectrum in both the home-setting and medical facility.

Trial registration number
ClinicalTrials.gov NCT03054181
The Devil’s in the Detail: Implementation Fidelity in Evaluating a School-Based Prevention Programme for Children Under 12
Authors: Kurdi Zain , Millar Annemarie , Barter Christine Anne , Stanley Nicky 
Published: April 2023

Abstract
Purpose
This paper examines implementation fidelity (IF) and the underpinning conceptual framework drawing on the evaluation of a UK-wide, manualized child abuse and neglect prevention program for elementary schools. We describe and assess our approach to assessing IF and consider how IF can inform program development.

Method
We drew on the literature on program fidelity and critical components of the program evaluated to identify three dimensions of IF: Coverage, Quality and Context. Data was collected through external observations using systemized observation schedules which were extracted to be scored using scoring protocols for each intervention type. Scores were calculated by two researchers with a random sample cross-checked by a third member of the research team.

Results
Observation analysis demonstrated consistency in the coverage of content when delivering assemblies for both younger and older children with at least 76% coverage of content across the assemblies. However, observation analysis revealed greater levels of variability in the delivery of workshops. Material on sexual abuse was less fully covered and children reported that some facilitators lacked confidence and clarity in delivering this material (Stanley et al., 2023).

Conclusion
Our results indicate the usefulness of systemized observations in capturing coverage of content, these findings underscore the importance of developing scoring protocols and training observers prior to evaluating program delivery. We highlight the significance of integrating implementation fidelity training for program facilitators and implementers to both assist with monitoring and to maintain quality, despite variations in the actual delivery and setting of the program.
A Decision Aid to Support Vocational Rehabilitation Professionals Offering Tailored Care to Benefit Recipients with a Long-Term Work Disability: A Feasibility Study
Authors: de Geus Christa J. C. , Huysmans Maaike A. , van Rijssen H. Jolanda , Juurlink Trees T. , de Maaker-Berkhof Marianne , Anema Johannes R. 
Published: April 2023

Abstract
Purpose
This feasibility study focusses on the implementation and use of a decision aid, which supports vocational rehabilitation (VR) professionals in helping clients with a disability pension return to work in practice. The decision aid shows an overview of the clients’ return to work barriers and suggests suitable VR interventions based on these barriers.

Methods
The study population consisted of VR professionals working at the Dutch Social Security Institute and their clients receiving a (partial) work disability pension. The feasibility was measured with concepts of the Linnan and Steckler framework and the attitude, social norm and self-efficacy model. Data were collected using questionnaires, checklists and qualitative interviews.

Results
Ten professionals participated in this study. Fifty-four clients were asked to fill in the questionnaire of the decision aid and 32 clients received VR care based on the decision aid. In general, VR professionals and clients were satisfied with the decision aid and perceived a few barriers for using the decision aid.

Conclusions
This study showed that it is feasible to implement and use the decision aid. To improve the implementation of this decision aid, it should be implemented in digital systems used by professionals to improve efficiency of working with the decision aid.