bluuebunny/crossref_metadata_2025_split huggingface dataset:
Potentiation of host defense through sRNA packaged in OMVs ofXanthomonas oryzaepv.oryzicola
Authors: Wu Yan , Wang Sai , Wang Peihong , Nie Wenhan , Ahmad Iftikhar , Chen Gongyou , Zhu Bo 
Published: March 2023

AbstractBacterial outer membrane vesicles (OMVs)-packaged delivery of noncoding small RNAs (sRNAs) can function as novel mediators of interspecies communication. However, the role of which in the interaction between phytopathogenic bacteria and their host plants is unclear. In this study, we characterized differentially packaged sRNAs inXanthomonas oryzaepv.oryzicola(Xoc) BLS256 OMVs using RNA-Seq, and we selected the most abundant sRNA Xosr001 for further study based on its essential role in the induction of stomatal immunity in rice.Xocloads Xosr001 into OMVs, which are transferred specifically into the mechanical tissues of rice leaves. We uncovered that OMVs-mediated Xosr001 inhibitors attenuatedOsJMT1transcripts in vivo and reduced the endogenous MeJA contents in rice leaves. Stomatal conductance was measured to show that ΔXosr001 mutant weakened the ability of stomatal re-opening on rice leaves after spray inoculation. Most notably, the transgenic rice lines OsJMT1-HA-OE exhibited attenuated stomatal immunity and disease susceptibility after ΔXosr001 infection compared withXocinfection. These results define that Xosr001 packaged inXocOMVs highlights a smart molecular mechanism to activate stomatal immunity in rice.
Extraocular muscle stem cells exhibit distinct cellular properties associated with non-muscle molecular signatures
Authors: Di Girolamo Daniela , Benavente-Diaz Maria , Grimaldi Alexandre , Lopes Priscilla Thomas , Murolo Melania , Evano Brendan , Gioftsidi Stamatia , Laville Vincent , Mella Sebastian , Tajbakhsh Shahragim , Comai Glenda 
Published: March 2023

AbstractThe muscle stem cell (MuSC) population is recognized as functionally heterogeneous. Cranial muscle stem cells, which originate from head mesoderm, can have greater proliferative capacity in culture and higher regenerative potential in transplantation assays when compared to those in the limb. The existence of such functional differences in phenotypic outputs remain unresolved as a comprehensive understanding of the underlying mechanisms is lacking. We addressed this issue using a combination of clonal analysis, live imaging, and scRNA-seq, identifying critical biological features that distinguish extraocular (EOM) and limb (Tibialis anterior, TA) MuSC populations. Time-lapse studies using aMyogenintdTomatoreporter showed that the increased proliferation capacity of EOM MuSCs is accompanied by a differentiation delayin vitro. Unexpectedly, in vitro activated EOM MuSCs expressed a large array of distinct extracellular matrix (ECM) components, growth factors, and signaling molecules that are typically associated with mesenchymal non-muscle cells. These unique features are regulated by a specific set of transcription factors that constitute a coregulating module. This transcription factor network, which includes Foxc1 as one of the major players, appears to be hardwired to EOM identity as it is present in quiescent adult MuSCs, in the activated counterparts during growth and retained upon passages in vitro. These findings provide insights into how high-performing MuSCs regulate myogenic commitment by active remodeling of their local environment.
2’-Fucosyllactose helps butyrate producers outgrow competitors in infant gut microbiota simulations
Authors: Versluis David M. , Schoemaker Ruud , Looijesteijn Ellen , Geurts Jan M. W. , H. Merks Roeland M. 
Published: March 2023

AbstractA reduced capacity for butyrate production by the early infant gut microbiota is associated with negative health effects, such as inflammation and the development of allergies. Here we develop new hypotheses on the effect of the prebiotic galacto-oligosaccharides (GOS) or 2’-fucosyllactose (2’-FL) on butyrate production by the infant gut microbiota using a multiscale, spatiotemporal mathematical model of the infant gut. The model simulates a community of cross-feeding gut bacteria at metabolic detail. It represents the gut microbiome as a grid of bacterial populations that exchange intermediary metabolites, using 20 different subspecies-specific metabolic networks taken from the AGORA database. The simulations predict that both GOS and 2’-FL promote the growth ofBifidobacterium, whereas butyrate producing bacteria are only consistently abundant in the presence of propane-1,2-diol, a product of 2’-FL metabolism. The results suggest that in absence of prebiotics or in presence of only GOS, bacterial species, includingCutibacterium acnesandBacteroides vulgatus, outcompete butyrate producers by feeding on intermediary metabolites. In presence of 2’-FL, however, production of propane-1,2-diol specifically supports butyrate producers.
Human immunodeficiency virus (HIV) dynamics in secondary lymphoid tissues and the evolution of cytotoxic T lymphocyte (CTL) escape mutants
Authors: Chung Wen-Jian , Connick Elizabeth , Wodarz Dominik 
Published: March 2023

AbstractIn the secondary lymphoid tissues, human immunodeficiency virus (HIV) can replicate both in the follicular and the extrafollicular compartments. Yet, virus is concentrated in the follicular compartment in the absence of antiretroviral therapy, in part due to the lack of cytotoxic T lymphocyte (CTL)-mediated activity there. CTL home to the extrafollicular compartment, where they can suppress virus load to relatively low levels. We use mathematical models to show that this compartmentalization can explain seemingly counterintuitive observations. First, it can explain the observed constancy of the viral decline slope during antiviral therapy irrespective of the presence of CTL in SIV-infected macaques, under the assumption that CTL-mediated lysis significantly contributes to virus suppression. Second, it can account for the relatively long times it takes for CTL escape mutants to emerge during chronic infection even if CTL-mediated lysis is responsible for virus suppression. The reason is the heterogeneity in CTL activity, and the consequent heterogeneity in selection pressure between the follicular and extrafollicular compartments. Hence, to understand HIV dynamics more thoroughly, this analysis highlights the importance of measuring virus populations separately in the extrafollicular and follicular compartments rather than using virus load in peripheral blood as an observable; this hides the heterogeneity between compartments that might be responsible for the particular patters seen in the dynamics and evolution of the HIVin vivo.
The Evolution of Centriole Degradation in Mouse Sperm
Authors: Khanal Sushil , Jaiswal Ankit , Chowdanayaka Rajanikanth , Puente Nahshon , Turner Katerina , Assefa Kebron Yeshitela , Nawras Mohamad , Back Ezekiel David , Royfman Abigail , Burkett James P. , Cheong Soon Hon , Fisher Heidi S. , Sindhwani Puneet , Gray John , Basappa Ramachandra Nallur , Avidor-Reiss Tomer 
Published: March 2023

AbstractCentrioles are subcellular organelles found at the cilia base with an evolutionarily conserved structure and a shock absorber-like function. In sperm, centrioles are found at the flagellum base and are essential for embryo development in basal animals. Yet, sperm centrioles have evolved diverse forms, sometimes acting like a transmission system, as in cattle, and sometimes becoming dispensable, as in house mice. How the essential sperm centriole evolved to become dispensable in some organisms is unclear. Here, we test the hypothesis that this transition occurred through a cascade of evolutionary changes to the proteins, structure, and function of sperm centrioles and was possibly driven by sperm competition. We found that the final steps in this cascade are associated with a change in the primary structure of the centriolar luminal protein FAM161A in rodents. This information provides the first insight into the molecular mechanisms and adaptive evolution underlying a major evolutionary transition within the internal structure of the mammalian sperm neck.Graphical abstractHighlights– Barrel-shaped centrioles are present in all rodents except members of the Muridae taxonomical family.– The FAM161A sequence evolved selectively in rodents and specifically in a murid subgroup.– The evolution of centriole degradation is correlated with the expression of a novel FAM161A isoform.– The ancestral centriole remodeling program involves elongation followed by redistribution.– The mouse centriole degradation program involves elongation followed by elimination.
A Living Organoid Biobank of Crohn’s Disease Patients Reveals Molecular Subtypes for Personalized Therapeutics
Authors: Tindle Courtney , Katkar Gajanan D. , Fonseca Ayden G. , Taheri Sahar , Lee Jasper , Maity Priti , Sayed Ibrahim M. , Ibeawuchi Stella-Rita , Vidales Eleadah , Pranadinata Rama F. , Fuller Mackenzie , Stec Dominik L. , Anandachar Mahitha Shree , Perry Kevin , Le Helen N. , Ear Jason , Boland Brigid S. , Sandborn William J. , Sahoo Debashis , Das Soumita , Ghosh Pradipta 
Published: March 2023

ABSTRACT (Structured)Crohn’s disease (CD) is a complex, clinically heterogeneous disease of multifactorial origin; there is no perfect pre-clinical model, little insight into the basis for such heterogeneity, and still no cure. To address these unmet needs, we sought to explore the translational potential of adult stem cell-derived organoids that not only retain their tissue identity, but also their genetic and epigenetic disease-driving traits. We prospectively created a biobank of CD patient-derived organoid cultures (PDOs) using biopsied tissues from colons of 34 consecutive subjects representing all clinical subtypes (Montreal Classification B1-B3 and perianal disease). PDOs were generated also from healthy subjects. Comparative gene expression analyses enabled benchmarking of PDOs as tools for modeling the colonic epithelium in active disease and revealed that despite the clinical heterogeneity there are two major molecular subtypes: immune-deficient infectious-CD [IDICD] and stress and senescence-induced fibrostenotic-CD [S2FCD]. The transcriptome, genome and phenome show a surprising degree of internal consistency within each molecular subtype. The spectrum of morphometric, phenotypic, and functional changes within the “living biobank” reveals distinct differences between the molecular subtypes. These insights enabled drug screens that reversed subtype-specific phenotypes, e.g., impaired microbial clearance in IDICD was reversed using agonists for nuclear receptors, and senescence in S2FCD was rectified using senotherapeutics, but notvice versa. Phenotyped-genotyped CD-PDOs may fill the gap between basic biology and patient trials by enabling pre-clinical Phase ‘0’ human trials for personalized therapeutics.GRAPHIC ABSTRACTIn BriefThis work creates a prospectively biobanked phenotyped-genotyped Crohn’s disease patient-derived organoids (CD-PDOs) as platforms for molecular subtyping of disease and for ushering personalized therapeutics.HIGHLIGHTSProspectively biobanked CD-organoids recapitulate the disease epithelium in patientsThe phenome-transcriptome-genome of CD-organoids converge on two molecular subtypesOne subtype shows impaired microbial clearance, another increased cellular senescencePhenotyped-genotyped PDOs are then used for integrative and personalized therapeutics
Age-related brain mechanisms underlying short-term recognition of musical sequences: An EEG study
Authors: Costa M. , Vuust P. , Kringelbach M.L. , Bonetti L. 
Published: March 2023

AbstractRecognition is the ability to correctly identify previously learned information. It is an important part of declarative episodic memory and a vital cognitive function, which declines with ageing. Several studies investigated recognition of visual elements, complex images, spatial patterns, and musical melodies, focusing especially on automatic and long-term recognition. Here, we studied the impact of ageing on the event-related potentials using electroencephalography (EEG) associated with short-term recognition of auditory sequences. To this end, we recruited 54 participants, which were divided into two groups: (i) 29 young adults (20-30 years old), (ii) 25 older adults (60-80 years old). We presented two sequences with an interval of a few seconds. Participants were asked to state how similar the second sequence was with regards to the first one. The neural results indicated a stronger negative, widespread activity associated with the recognition of the same sequence compared to the sequences that were transposed or completely different. This difference was widely distributed across the EEG sensors and involved especially temporo-parietal areas of the scalp. Notably, we reported largely reduced neural responses for the older versus young adults, even when no behavioral differences were observed. In conclusion, our study suggests that the combination of auditory sequences, music, and fast-scale neurophysiology may represent a privileged solution to better understand short-term memory and the cognitive decline associated with ageing.
Spectral detection of condition-specific biological pathways in single-cell gene expression data
Authors: Chin Wee Loong , dos Santos Leonardo Portes , Small Michael , Lesterhuis W. Joost , Lassmann Timo 
Published: March 2023

AbstractSingle cell RNA sequencing is an ubiquitous method for studying changes in cellular states within and across conditions. Differential expression (DE) analysis may miss subtle differences, especially where transcriptional variability is not unique to a specific condition, but shared across multiple conditions or phenotypes. Here, we present CDR-g (Concatenate-Decompose-Rotate genomics), a fast and scalable strategy based on spectral factorisation of gene coexpression matrices. CDR-g detects subtle changes in gene coexpression across a continuum of biological states in multi-condition single cell data. CDR-g collates these changes and builds a detailed profile of differential cell states. Applying CDR-g, we show that it identifies biological pathways not detected using conventional DE analysis and delineates novel, condition-specific subpopulations in single-cell datasets.
Assessing the strategic levers in the discourse of best-performing CEOs: a three-dimensional model
Authors: Galan-Cubillo Javier , Garcia-Ortega Beatriz , de-Miguel-Molina Blanca 
Published: March 2023

PurposeThe main purpose of this paper is to assess the patterns in the public discourse of successful chief executive officers (CEOs) in terms of performance, with the CEO's strengths and aspects to improve.Design/methodology/approachThis paper aligns with the literature that appraises CEO public discourse and relevance. From the literature review, the strategic levers in CEO discourse toward high performance are identified. The CEO letters in the period 2017–2019 of the top 25 best performing CEOs (BPCs) according to Harvard Business Review ranking 2019 are qualitatively examined through a multiple close reading analytical technique and multiple correspondence analysis (MCA) is applied to assess the patterns.FindingsThe paper delivers a three-dimensional model representing how the identified strategic levers are articulated by BPCs in the BPC's discourse following diverse patterns. This paper points out BPC's strengths, among them a high level of moral reasoning compared to previous studies and improvable areas such as the extended absence of autocritique at the firm and personal level or the lack of leverage on the need for agility and proactive adaption.Practical implicationsThis paper contributes further CEO awareness of the strategic role of the discourse and offers clues to enhance CEO awareness, as well as criteria for boards of directors to appraise CEO discourse.Originality/valueAdopting a novel approach, this paper addresses the strategic levers triggered by CEOs in their letters from a managerial implication perspective, providing relevant theoretical insight on how they are articulated.
A study on mediation effect of relationship quality outcome between customer relationship management and mutual fund decision
Authors: Deb Suman Kumar , Jain Ruchi , Manohar Sridhar , Marwah Sanjiv 
Published: March 2023

Purpose
Usage of updated technology is continuously empowering customer relationship management (CRM) to be convenient and user friendly, where customers are kept engaged with knowledge and information. This enables them on decision-making and managing their portfolio, especially in mutual fund investments. To improve toward a positive decision, certain quality related variables needed to be considered. Thus, this study aims to estimate the mediation effect of relationship quality and outcome (RQO) between CRM and investment decision-making in mutual funds (MFD).

Design/methodology/approach
The descriptive study adopted the constructs from existing empirical literatures to conceptualize the model with three higher order constructs with 12 dimensions. Survey method is used, and with a structured questionnaire, a total of 323 mutual fund investors were approached using nonprobability criterion sampling technique, of which 262 relevant responses were considered for estimating the structural model. Smart PLS was used to establish the relationship of the constructs.

Findings
The result emphasizes a significant direct and indirect relationship indicating that investors are more inclined to MFD through technology-enabled CRM and RQO plays a vital role in explaining the direct relationship between CRM and MFD. The results of the study are in-line with the existing literature.

Practical implications
The study highlights that financial institutions must focus not only on technological diffusion but also needs to ensure quality service by providing knowledge and information during every access of transactions by customers, making them independent and confident during investments.

Originality/value
This study indicates how capacity efficiency, which is a part of service productivity, can be managed without affecting the outcome efficiency by incorporating technology in the place of human interaction during relationship acquiring and retaining process.
Effects of reputation on guest satisfaction: from the perspective of two-sided reviews on Airbnb
Authors: Ye Qiang , Liang Sai , Wei Zaiyan , Law Rob 
Published: March 2023

Purpose
From the perspective of two-sided review systems, this study aims to investigate how guests’ prior reputation influences their subsequent satisfaction on Airbnb.

Design/methodology/approach
This study applied a conceptual framework based on social capital theory to explain the effect of guests’ reputation decided by hosts’ prior evaluations on their subsequent satisfaction. The authors collected 96,204 guest reviews posted for 17,325 properties on Airbnb and used the review polarity to measure guest satisfaction. All historical evaluations generated by hosts for each guest were collected and treated as a proxy of guest reputation. Ordinary least squares regressions were conducted to estimate the effect of guests’ reputation on their subsequent satisfaction.

Findings
Results show that guests whose historical evaluations have higher valences or larger variations tend to be more satisfied in their subsequent bookings. However, the number of reviews that guests received from hosts in the past does not influence their subsequent satisfaction.

Research limitations/implications
This study provides new insights into the hospitality literature by identifying the influencing factors of guest satisfaction on peer-to-peer rental platforms from the perspective of two-sided review systems. Results also present practical implications to property owners and website designers to gain a deeper understanding of the determinants of guest satisfaction and the consequences of social interactions between hosts and guests.

Originality/value
This study is a novel attempt that analyzes the effect of guests’ reputation on their satisfaction with subsequent bookings based on two-sided review systems on peer-to-peer rental platforms. Thus, this study provides a starting point for investigating how two-sided review systems affect use behavior on peer-to-peer rental platforms.
The impact of transformational leadership on challenging influence strategies: an approach using structural equation models
Authors: López-Lemus Jorge Armando , De la Garza Carranza María Teresa 
Published: March 2023

Purpose
The purpose of this study is to identify the impact that transformational leadership has on challenging influence strategies in business leaders of the micro, small and medium-sized enterprises (MSMEs) of the state of Guanajuato, Mexico.

Design/methodology/approach
The methodological design was quantitative, explanatory, observational and cross-sectional, where a sample of 343 leaders from the business sector of the MSMEs of the state of Guanajuato, Mexico was obtained. To check the hypotheses, a structural equation model (SEM) was developed using the AMOS v.21 statistical software. For the analysis of the data, SPSS v.21 was used. Regarding the goodness and fit indexes of the SEM (χ2 = 595,133 df = 244; CFI = 0.914 and TLI = 0.903; RMSEA = 0.06), which were acceptable (Jöreskog and Sörbom, 1981; López-Lemus and Zavala, 2019; Rigdon, 1996; Tucker and Lewis, 1973).

Findings
The results obtained through the present study, it is shown that leadership and transformational influences positively and significantly on challenging influence strategies: rational (β1 = 0.53, p < 0.01), inspiring (β2 = 0.65, p < 0.01) as well as the strategy of participatory influence (β3 = 0.62, p < 0.01) of the business leaders of the MSMEs of the state of Guanajuato, Mexico.

Originality/value
The findings are relevant and of great value because there is currently not enough research that focuses on the variables analysed on the leadership and influence strategies of business leaders of the MSMEs in the context of the state of Guanajuato, Mexico.
Perceived organizational politics, organizational disidentification and counterproductive work behaviour: moderating role of external crisis threats to work
Authors: De Clercq Dirk , Pereira Renato 
Published: March 2023

Purpose
The purpose of this study is to unpack the relationship between employees’ perceptions of organizational politics and their counterproductive work behaviour, by postulating a mediating role of organizational disidentification and a moderating role of perceived external crisis threats to work.

Design/methodology/approach
The empirical assessment of the hypotheses relies on survey data collected among employees who work in a large banking organization.

Findings
Perceptions that organizational decision-making is marked by self-serving behaviour increase the probability that employees seek to cause harm to their employer, because they feel embarrassed by their organizational membership. This mediating role of organizational disidentification is especially prominent when they ruminate about the negative impact of external crises on their work.

Practical implications
This study details an important danger for employees who feel upset with dysfunctional politics: They psychologically distance themselves from their employer, which then prompts them to formulate counterproductive responses that likely make it more difficult to take on the problem in a credible manner. This detrimental dynamic is particularly risky if an external crisis negatively interferes with their work functioning.

Originality/value
This study adds to prior research by detailing an unexplored but relevant mechanism (organizational disidentification) and moderator (external crisis threats) by which perceived organizational politics translates into enhanced counterproductive work behaviour.