bluuebunny/crossref_metadata_2025_split huggingface dataset:
A comparison of Patient-Reported Outcomes and Post-Operative complications between Suture-Augmented and Standard Hamstring Autograft Single-Bundle ACL Reconstruction: a retrospective cohort study
Authors: Darestani Reza Tvakoli , Afzal Sina , baroutkoub Mojtaba , Barati Hassan , Sayyadi Shahram , Pourmojarab Ali 
Published: May 2023

Abstract
Background Augmentation of the biologic graft with nonabsorbable suture material during anterior cruciate ligament reconstruction (ACLR) is a relatively new technique to enhance its biomechanical properties and add additional support during the critical process of healing. We aim to compare the short-term functional patient-reported outcome measures (PROMs) and complication rates of patients treated with either standard single-bundle four-strand hamstring ACLR or added suture augmentation (SA).
Methods: Patients undergoing arthroscopic ACLR between February 2015 and September 2019 in the standard ACLR group (79 patients) and the SA-ACLR group (90 patients) operated by adding a no.5 FiberWire® (Arthrex, Naples, FL, USA) braided suture to the hamstring autograft were retrospectively comared together in this study. Patients were followed up for a 24-month period and PROMs were assessed and compared by the International Knee Documentation Committee (IKDC) Subjective Knee Form and Tegner-Lysholm knee score. Patients’ demographic and clinical characteristics and postoperative complications including graft retear requiring revision surgery, deep vein thrombois, and surgical site infection were recorded and analyzed.
Results Mean age was 31.6 ± 8.3 in standard ACLR and 30.5 ± 7.6 in SA-ACLR. There was no statistically significant difference between the two groups in terms of age, sex, body mass index, and medical comorbidities. The values of the IKDC scores increased to 75.8 ± 18.9 in the standard ACLR group, and 85.6 ± 12.6 in the SA-ACLR group, 24 months after the operation (P<0.05). The 24-month postoperative Tegner-Lysholm scores escalated to 79.3 ± 21.0 in the standard ACLR group and 91.0 ± 13.7 in the SA-ACLR group (P<0.05). Four (5.1%) patients in the standard ACLR group and 4 (4.4%) in the SA-ACLR group experienced graft retear requiring revision surgery (P>0.05). Surgical site infection and deep vein thrombosis showed no significant difference between the two groups two years after ACLR.
Conclusion SA-ACLR is associated with improved short-term functional PROMs compared to the standard hamstring ACLR. Although SA did not reduce retear rate, and infection and DVT were not differ between study groups, superior improvement of PROMs in SA approach, leverages this method for ACLR.
Parabens and Paraben Transformation Products in The Brazos River (Texas, USA) Before and After Wastewater Treatment
Authors: Penrose Michael , Cobb George 
Published: May 2023

Abstract
Parabens are ubiquitous, being found in surface waters around the world. Although little is known about the release of paraben transformation products and fate of transformation products in surface water. This study evaluates both parabens and paraben transformation products in the Brazos River upstream and downstream of a wastewater facility located in Waco, Texas. Concentrations of thirteen compounds were reported in this study, five parent parabens and eight paraben disinfection byproducts. Analyte concentrations were spatially evaluated to determine if release of wastewater effluent effects their concentrations in the river. Two Brazos River tributaries were also sampled to determine if they released parabens and related compounds to the Brazos. Sampling occurred weekly for one year with at least 40 samples collected at each site. Analyses were completed for both yearly and seasonal data. Sites downstream of wastewater treatment outfalls had lower concentrations of methyl paraben during the yearly analysis and across multiple seasons in the seasonal analysis. Para-hydroxybenzoic acid was the compound present in greatest concentration at most sites across most seasons, and spatial changes in para-hydroxybenzoic acid varied by season, with no identifiable trends. Dichlorinated paraben concentrations increased in the river at sites downstream of wastewater treatment. Concentration increases indicate that wastewater effluent contains sufficiently high dichlorinated paraben concentrations to effect concentrations downstream of effluent discharges. Dichlorinated species also persisted in the environment, with no significant decreases at sites further downstream during any season. Methyl paraben concentrations decreased at the site furthest downstream while dichlorinated methyl paraben concentrations remained stable showing that the dichlorinated species degrade slower than their respective parent paraben. Due to the dichlorinated species being released in higher concentrations in effluent than parents and being more resistant to degradation, the dichlorinated parabens are more likely to environmentally relevant than are parent parabens.
mCherry Fusion Proteins Facilitate Production of Recombinant,Cysteine-Rich Leptospira interrogans Proteins in Escherichia coli
Authors: Chaurasia Reetika , Liang Cathleen , How Kenneth , Vieira Dielson S. , Vinetz Joseph M. 
Published: May 2023

Abstract
Background Recombinant fluorescent fusion proteins are fundamental to advancing many aspects of protein science. Such proteins are typically used to enable the visualization of functional proteins in experimental systems, particularly cell biology. An important problem in biotechnology is the production of functional, soluble proteins. Here we report the use of mCherry-fusions of soluble, cysteine-rich, Leptospira-secreted exotoxins in the PF07598 gene family, the so-called virulence modifying (VM) proteins.
Results The mCherry fusion proteins facilitated the production of the VM proteins (LA3490 and LA1402) by enabling the visual detection of pink colonies and following them through lysis and sequential chromatography steps. CD-spectroscopy analysis confirmed the stability and robustness of the mCherry-fusion protein, with a structure comparable to AlphaFold structural predictions. LA0591, a unique member of the PF07598 gene family that lacks N-terminal ricin B-like domains, was produced as a tagless protein that strengthens the recombinant protein production protocol. The current study provides the approaches for the synthesis of 50-125 kDa soluble, cysteine-rich, high-quality mCherry tagged or tagless fast protein liquid chromatography (FPLC)-purified protein.
Conclusions The use of mCherry-fusion proteins enables a streamlined, efficient process of protein production and qualitative and quantitative downstream analytical and functional studies. Approaches for troubleshooting and optimization were systemically evaluated to overcome difficulties in recombinant protein expression and purification, demonstrating biotechnology utility in accelerating recombinant protein production.
Discrepancy in the reports on life events between parents and their depressed/anxious children leads to severer psychopathology and lower responsiveness to SSRI treatment
Authors: Amitai Maya , etedgi elhai , mevorach tomer , kalimi roni , horesh netta , oschry noga , apter alan , benaroya noa , fennig silvana , weizman abraham , chen alon 
Published: May 2023

Abstract
Introduction: Exposure to a range of stressful life events (SLE) is implicated in youth psychopathology. Discrepancy between parents’/children’s’ reports (especially regarding SLE) is a major concern in child psychiatry. This study was designed to assess parent–youth discrepancies regarding SLE and its association with severity of psychopathology at baseline and response to treatment. Additionally, we assessed the association between three plasma pro-inflammatory cytokine levels and SLE.
Methods: SLE were assessed in children/adolescents suffering from depressive/anxiety disorders using the life events checklist (LEC), a self-report questionnaire measuring the impact of negative life events (NLE) and positive life events (PLE), as reported by the children and their parents. Severity of depression/anxiety disorders and response to antidepressant treatment were evaluated and correlated with both measures of LEC. We also corelated SLE with levels of three pro-inflammatory cytokines (TNF-α, IL-6, IL-1β).
Results: Participants were 96 parent-child dyads (39 boys, 57 girls) aged 6-18 (mean=13.90, SD=2.41y). Parents reported higher severity of NLE than their children. Discrepancy in PLE was associated with more severe psychopathology and reduced response to treatment. No association with cytokine levels was found.
Discussion: Discrepancy in informant reports regarding life events in depressed/anxious youth, especially regarding PLE, is associated with more severe psychopathology and reduced response to pharmacotherapy. It is important to increase congruency regarding SLE between parents and children to improve response to treatment.
Development of a Sensitive Monoclonal Antibody-Based Lateral Flow Immunoassay for the Detection of Benzoic Acid in Liquid Food
Authors: Zhao Qiuyan , Ding Mingyue , Pei Dangshuai , Qi Xiuhua , Mao Yexuan , Huang Xianqing , Song Lianjun , Zuo Jingnan , Zhang Xiya , Yang Huijuan 
Published: May 2023

Abstract
To monitor benzoic acid (BA) residues in liquid food samples, a monoclonal antibody (mAb)-based lateral flow immunoassay (LFA) was developed in this study. First, 2-aminobenzoic acid (2-AA), 3-aminobenzoic acid (3-AA), and 4-aminobenzoic acid (4-AA) were conjugated to BSA and used as immunogens. After cell fusion, mAb 6D8 from 4-AA-BSA performed best with an IC50 value of 0.21 mg/L using 3-AA-OVA as heterogeneous antigen, which represented a 3.4-fold improvement compared with the homogeneous antigen 4-AA-BSA. Subsequently, eight kinds of CGNPs with sizes varying from 20.94 nm to 90.00 nm were synthesized for screening the suitable size to develop a sensitive LFA. Finally, a sensitive LFA based on colloidal gold (23.27nm) nanoparticles was developed for screening BA with a cut-off value of 4 mg/L, which could meet the requirement of BA detection in milk, Fanta, Sprite, Coca-Cola, and Stmart samples.
Featured immune characteristics of COVID-19 and systemic lupus erythematosus revealed by multidimensional integrated analyses
Authors: Zhao Xingwang , Zhang Mengjie , Jia Yuying , Liu Wenying , Li Shifei , Gao Cuie , Zhang Lian , Ni Bing , Ruan Zhihua , Dong Rui 
Published: May 2023

Abstract
Coronavirus disease 2019 (COVID-19) shares similar immune characteristics with autoimmune diseases like systemic lupus erythematosus (SLE). However, such associations have not yet been investigated at the single-cell level. Thus, in this study, we integrated and analyzed RNA sequencing results from different patients and normal controls from the GEO database and identified subsets of immune cells that might involve in the pathogenesis of SLE and COVID-19. We also disentangled the characteristic alterations in cell and molecular subset proportions as well as gene expression patterns in SLE patients compared with COVID-19 patients. Key immune characteristic genes (such as CXCL10 and RACK1) and multiple immune-related pathways (such as the coronavirus disease-COVID-19, T-cell receptor signaling, and MIF-related signaling pathways) were identified. We also highlighted the differences in peripheral blood mononuclear cells (PBMCs) between SLE and COVID-19 patients. Moreover, we provided an opportunity to comprehensively probe underlying B-cell‒cell communication with multiple ligand‒receptor pairs (MIF-CD74 + CXCR4, MIF-CD74 + CD44) and the differentiation trajectory of B-cell clusters that is deemed to promote cell state transitions in COVID-19 and SLE. Our results demonstrate the immune response differences and immune characteristic similarities, such as the cytokine storm, between COVID-19 and SLE, which might pivotally function in the pathogenesis of the two diseases and provide potential intervention targets for both diseases.
Application of impulse oscillometry to detect interstitial lung disease and airway disease in adults with rheumatoid arthritis
Authors: Cheng Wen-Chien , Chang Shih-Hsin , Chen Wei-Chun , Wu Bing-Ru , Chen Chia-Hung , Lin Chi-Chien , Hsu Wu-Huei , Lan Joung-Liang , Chen Der-Yuan 
Published: May 2023

AbstractBackground We conducted a retrospective observational study to explore the potential application of impulse oscillometry (IOS) as an alternative to high-resolution computed tomography (HRCT) for detecting pulmonary involvement in patients with rheumatoid arthritis (RA) because clinically evident interstitial lung disease (ILD) and airway involvement are common in this population. Methods We enrolled 72 patients with RA who underwent pulmonary function tests (PFTs) and IOS between September 2021 and September 2022. We aimed to identify the PFT and IOS variables associated with lung diseases shown on HRCT images. Results In our cohort of 72 patients, 48 underwent HRCT; of these, 35 had airway disease or ILD and 13 showed no obvious abnormalities on HRCT. Abnormal IOS and PFT parameters were observed in 34 and 23 patients, respectively, with abnormal HRCT images. The predicted percentages for forced vital capacity, the ratio of forced expiratory volume in the first one second to forced vital capacity, and forced mid-expiratory flow value were significantly lower in patients with abnormal HRCT. Lung resistance at 5 Hz, difference in resistance between 5 and 20 Hz, resonant frequency (Fres), and reactance area were higher in these patients and reactance at 5 Hz was lower. Compared to other parameters, Fres > 14.14 was significantly associated with alterations in HRCT and may be used as an indicator for monitoring disease. Conclusion Fres > 14.14 is significantly associated with lung involvement in RA patients. Performance of spirometry with IOS is more beneficial than spirometry alone for evaluating lung involvement in RA patients.
Effect of partial delignification on defibrillation of Brassica Campestris straw and its performance in transparent film
Authors: Xiao Peng , Huang Dayong , Rahman Md Mostafizur , Wu Min , Kuga Shigenori , Huang Yong 
Published: May 2023

Abstract
The study on preparation of lignin-containing cellulose nanofiber (LCNF) with high-yield, high-efficient, low-cost is a great challenge in large-scale production. Regulating hydrogen-bonding network by changing the content of lignin is of great significance to improve the efficiency of defibrillation of cellulose. In this paper, the LCNF with high yield (91.55%) was fabricated by oxidizing Brassica Campestris (BC) straw to partially remove the lignin, followed by ball-milling and high-density ultrasonic treatment. The atomic force microscope results reveal that LCNF exhibit fiber network structure with a typical diameter of 2-5 nm similar to those of elementary crystallites. Variations in the structure and hydrogen bonding characteristics of the products are further determined based on the FT-IR results followed deconvolution fitting, showing that intra and intermolecular hydrogen-bonding energies and distances decrease with the increase of degree of oxidization. Furthermore, the LCNF films with high tensile strength (225.78 MPa), good UV-blocking (80%) and water-resistant were constructed by vacuum assisted filtration. This research will provide an effective and convenient approach to obtain the LCNF materials with high-yield and widen its application fields.
Profiling diagnostic Approach of Thoracic Malignancies in Indonesia’s National Cancer Centre
Authors: Hanafi Arif Riswahyudi , Hanif Muhammad Alfin , Pangaribuan Mariska T.G. , Ariawan Wily Pandu , Pradipta Jaka , Cahyanti Dian , Rayhani Farilaila , Sutandyo Noorwati , Kurniawati Sri Agustini , Setiawan Lyana , Diandra Alyssa , Hidayat Kusmantoro , Imelda Priscillia 
Published: May 2023

Abstract
Introduction
Diagnostic modalities for thoracic malignancies, especially for lung cancer, have a pivotal impact on further planning. Providing tools should be utilized efficiently to gain a highly positive diagnosis. Thus, we aim to describe the diagnostic approach applied in our population and how each tools frequently used and provide adequate results, so it can be used as the fundamental picture for other centers.
Methods
A cross-sectional study, involving data from 3661 diagnostic procedures at the Dharmais Cancer Hospital, National Cancer Center in Indonesia, from January 2018-December 2022. Data were from the medical record and analyzed with p < 0.05 was considered statistically significant.
Result
Brushing with bronchoscopy was the most performed with a positive diagnostic yield of 58.59% cytologically, and VAST were carried out less but obtained the highest positive diagnosis 100% histologically (p = 0.000). Both cytology and histology examination obtained adenocarcinoma as the most common diagnosis (63.80%). Biomolecular EGFR test was the most frequently applied on lung cancer patients (81.43%), besides ALK and PDL-1 (p = 0.000). EGFR and TP53 mutation were highly expressed with next-generation sequencing.
Conclusion
Multimodality diagnostic procedures should be considered for patients since tissue is an issue, both cytologically and histologically. Biomolecular reflex tests, such as ALK, EGFR, and PD-L1, are essential to apply in lung cancer, and co-mutations can be detected by NGS, such as EGFR-TP53.
A pyroptosis score-based model for prognosis prediction of esophageal squamous cell carcinoma
Authors: Wang Jianlin , Meng Jing , Sun Fei , Xu Tian , Luo Judong , Ni Xinye 
Published: May 2023

Abstract
Background: Emerging evidences have demonstrated the dual effect of pyroptosis in cancer progression, pro- or anti-tumorigenic roles in differential cancer types. This study aimed to identify prognostic molecules in esophageal squamous cell carcinoma (ESCC) based on the bioinformatic analysis of pyroptosis-related genes (PRGs) expression profiles.
Method:Firstly, the pyroptosis gene sets were collected from the MSigDB database, and the mRNA levels were detected in ESCC and normal tissue samples from TCGA. Consensus cluster analysis was conducted to classify patients into different clusters that varied significantly in overall survival times. A pyroptosis score (PYS)- mediate model was generated and the property was assessed in the validation set.
Results: Here, we found sixteen PRGs were dysregulated in ESCC samples. The subtyping of ESCC based on differential expressed PRGs could classify patients into three clusters. A prognostic gene signature comprising two genes (HLA-E and TNFSF10) in the three clusters was established, and samples could be classified to two PYS groups. The PYS model displayed excellent performance in prognostic prediction both in the training and validation set. Patients in high PYS group implied universal inflammatory cell infiltration and the better response of paclitaxel and methotrexate (P < 0.05).
Conclusion: A PYS-based risk model comprising two genes that could predict the prognosis of ESCC patients. Our finding demonstrated the correlation between pyroptosis, survival outcome, immunity, and chemotherapy response in ESCC.
Access to Essential and Innovative Anti-cancer Medicines: A Longitudinal Study in Nanjing, China
Authors: Cao ZhaoLiu , Wang Lili , Hu Yun , Bao Baiyi , Liu Xiaohua , Li Mengyuan , Wang Xiao , Liu Pingyu , Li Xin 
Published: May 2023

Abstract

Purpose To evaluate the availability, cost, affordability, and drug utilization of anti-cancer medicines in Nanjing, Jiangsu.Methods Based on the standard survey methods of World Health Organization/Health Action International(WHO/HAI), a longitudinal tracking investigation study was performed to collect information about 24 essential anti-cancer medicines (EAMs) and 17 innovative anti-cancer medicines (IAMs) in 26 healthcare institutions in Nanjing from 2016 to 2020. The availability, defined daily dose cost (DDDc), defined daily doses (DDDs) and affordability of EAMs and IAMs were investigated. Wilcoxon’s rank-sum test was used to analyze the available data of adjacent years to check whether there was a statistical difference.Results The availability of EAMs during the study period showed no significant changes in Nanjing, but the availability of IAMs showed a significant increase in 2018 and 2019 and tended to stabilize in 2020. For EAMs, the DDDc of lowest-priced generics (LPGs) showed no significant changes during the study period, and the DDDc of originator brands (OBs) and IAMs significantly decreased. The DDDs of EAMs (LPGs) showed a decreasing trend since 2016 and rose again in 2019. Overall, the DDDs of EAMs(LPGs) decreased by 25.18% between 2016 and 2020, but the proportion selected for clinical treatment remained at 67.35% in 2020. The DDDs of EAMs (OBs) and IAMs both showed an increasing trend year by year, with a proportional increase of 207.72% and 652.68%, respectively; but the proportion selected for clinical treatment was only 16.09% and 16.56% respectively in 2020. Under the standard set in this study, EAMs (LPGs) had good affordability for urban residents but poor affordability for rural residents; all IAMs were affordable to urban residents, but most IAMs(11/17) were not affordable to rural residents by 2020. However, the affordability of these drugs was constantly improving.Conclusions There were no significant changes in the availability and cost of EAMs (LPGs), whose lower prices showed better affordability, especially for urban residents. Although their relative change in drug utilization showed a decreasing trend, they still dominated clinical treatment. Driven by the national drug price negotiation (NDPN) policy, the availability of IAMs was on the rise. The prices of IAMs and EAMs (OBs) decreased year by year with significantly improving affordability, but the high prices of these drugs remained unaffordable for rural residents. It is necessary to further develop and strengthen policies for essential medicines procurement assessment to improve the accessibility of EAMs. Meanwhile, a comprehensive strategy is needed in the future to improve the affordability of IAMs.
Psychometric and Structural Properties of the Karolinska Exhaustion Disorder Scale: A 1,072-Patient Study
Authors: Lindsäter Elin , de Leur Jakob Clason van , Rück Christian , Hedman-Lagerlöf Erik , Bianchi Renzo 
Published: May 2023

Abstract
Objective. Exhaustion disorder is a stress-related diagnosis that was introduced in 2005 to the Swedish version of the International Statistical Classification of Diseases and Related Health Problems, 10th edition (ICD-10). The Karolinska Exhaustion Disorder Scale (KEDS) was developed to assess exhaustion disorder symptomatology. While the KEDS is intended to reflect a single construct and be used based on its total score, the instrument's characteristics have received limited attention. This study investigated the KEDS’s psychometric and structural properties in a large clinical sample.Methods. The study relied on data from 1,072 patients diagnosed with exhaustion disorder that were included in two clinical trials in Sweden. We investigated the dimensionality, homogeneity, and reliability of the KEDS using advanced statistical techniques, including exploratory structural equation modeling (ESEM) bifactor analysis.Results. A one-factor confirmatory analytic model exhibited a poor fit, suggesting at least a degree of multidimensionality. The ESEM bifactor analysis found the general factor to account for about 72% of the common variance extracted, with an omega hierarchical coefficient of 0.680. Thus, the ESEM bifactor analysis did not clearly support the scale’s essential unidimensionality. A homogeneity analysis revealed a scale-level H of only 0.296, suggesting that KEDS’s total scores do not accurately rank individuals on the latent continuum assumed to underlie the measure. The KEDS’s reliability was modest, signaling considerable measurement error.Conclusion. Findings reveal important limitations to the KEDS with possible implications for the status of exhaustion disorder as a nosological category.Trial registration: This study was pre-registered on Open Science Framework (osf.io) on April 24, 2022 (https://osf.io/p34sq/).