bluuebunny/crossref_metadata_2025_split huggingface dataset:
Production of Biodegradable Animal-Free Leather from Bacterial Cellulose Layer
Authors: Soni Riya , Sapte Sakhi , singh Ravi kant , Wani Minal , afreen huda 
Published: February 2023

Abstract

One of the most popular goods traded globally is leather and its by-products. Tanneries transform the principal industrial raw material into usable leather. Although the leather tanning industry primarily uses waste from the livestock industry, it also uses a variety of chemicals to transform the raw material into the completed product. This excessive use of chemicals is detrimental to the environment as it is one of the significant causes of pollution. To overcome this, we attempted to produce biodegradable leather using SCOBY in our experiment. A by-product of the fermentation of Kombucha tea, SCOBY is a cellulose biofilm harbouring a symbiotic culture of bacteria and yeast. Two sucrose sources were used - sugarcane (13–15% sucrose) and sugar (99.9% sucrose), and the resulting layers from both sources were compared based on various tests. Our results have demonstrated that leather out of SCOBY possesses durability and strength at various temperatures as well as biodegradability and water retention capacity. Conclusively, we have demonstrated that further tests on these materials will exemplify their viable use in our day-to-day life in the future and would serve as the best and the most cost-effective as well as a chemical-free alternative to traditional leather.
The Control Effect of Strike-slip Faults on Geothermal Resources: A Case Study of the Middle-high Temperature Hot Spring in Xianshuihe Fault Zone, the Eastern of Qinghai-Tibet Plateau
Authors: Zhao Xueqin , Zhu Yuyin , Jiang Yaoxi , He Yujiang , Wang Fudong , Chai Peiran , Zhu Heyuan , Li Wentao , Tang Jie 
Published: February 2023

Abstract
Based on analysis of the fault structure of the Xianshuihe Fault Zone (XFZ) in combination with the analysis of hydrogen and oxygen isotopes and hydrochemical characteristics of hot springs, the coupling mechanism of the strike slip fault and hot springs was studied. The XFZ is located in the high-temperature geothermal area in the eastern part of the Qinghai–Tibet Plateau. The results have shown that the hot spring in the XFZ is in strong alignment with the mechanical segment of the fault. This can be divided into three geothermal zones with pronounced heterogeneity in terms of isotopic composition and hydrochemical characteristics. Among them, the water δD-δ18O composition of hot springs in the Daofu hydrothermal area was the separation, the water δD-δ18O composition of hot springs in the Kangding hydrothermal area was relatively enrichment, and the water δD-δ18O composition of hot springs in the Moxi hydrothermal area was the enrichmentest. This has highlighted the difference in recharge sources (elevation) in the three hydrothermal areas. The water chemistry of the hot springs reflects significant differences in the mechanism of water–rock action among the three hydrothermal areas. The water chemistry of the hot springs in the Daofu and Kangding hydrothermal areas is predominantly influenced by the dissolution of silicate rocks, while that in the Moxi hydrothermal area is mainly influenced by the dissolution of carbonate rocks and evaporite salts. The analysis suggests that the large strike-slip faults are the main channels for deep hydrothermal upwelling and control the heat source of the geothermal zone on the western Sichuan Plateau. Meanwhile, the structures on both sides of the large faults constitute local water circulation channels.
Atlantic meridional overturning circulation increases flood risk along the United States southeast coast
Authors: Volkov Denis , Zhang Kate , Johns William , Willis Josh , Hobbs William , Goes Marlos , Zhang Hong , Menemenlis Dimitris 
Published: February 2023

Abstract
The system of oceanic flows constituting the Atlantic Meridional Overturning Circulation (AMOC) moves heat and other properties to the subpolar North Atlantic, controlling regional climate, weather, sea levels, and ecosystems. Climate models suggest a potential AMOC slowdown towards the end of the 21st century due to anthropogenic forcing, which would accelerate coastal sea level rise along the western boundary and dramatically increase coastal flood risk. While the slowdown has not been observed to date, we show here that the AMOC-induced intrinsic changes in gyre-scale heat content, superimposed on the global mean sea level rise, are already influencing the frequency of floods along the United States southeastern seaboard. We find that ocean heat convergence, being the primary driver for interannual sea level changes in the subtropical North Atlantic, has led to an exceptional gyre-scale warming and associated dynamic sea level rise since 2010, accounting for 30-50% of flood days in 2015-2020.
Effectiveness of voice therapy after recovery from COVID-19 infection: A Rare Case Report
Authors: Aghadoost Samira , Molazeinal Yasamin , Khoddami Seyyedeh Maryam , Aghajanzadeh Mahshid 
Published: February 2023

Abstract
Introduction: COVID-19 induces ear, nose, and throat (ENT) difficulties in nearly 57.4 percent of patients. In patients with COVID-19, voice disorder and dysphonia can be created by shortness of breath, upper airway congestion, and changes in the larynx.
Case Presentation: We aimed to study the effect of voice therapy (VT) on a woman who suffered from long-term post-COVID-19 dysphonia and recurrent coughs that interrupted the connected speech. This study is a single case report. The patient was a 33-year-old woman referred by a laryngologist to the VT clinic with the diagnosis of muscle tension dysphonia (MTD). All voice assessments were conducted during two sessions before and after 10 sessions of VT. The evaluations included laryngostroboscopy, perceptual voice assessment, and acoustic-aerodynamic voice measurement. After that, ten sessions of Eclectic VT were done by a voice therapist. Following VT, the results showed better findings in all aspects of voice assessments.
Conclusions: The results of this study showed improvement of different aspects of voice after Eclectic VT and also, and the effectiveness of VT for decreasing dysphonia post-COVID-19. Although significant improvement was achieved, there was some degree of dysphonia and recurrent coughs after VT. These findings indicated that some side-effects of COVID_19 are persistent even after VT. This case report can highlight the sensory-motor deficit hypothesis or damage in ACE2 receptor in the larynx after COVID_19, but it still needs more investigations.
Sequenced steering vector estimation for eigen-subspace projection robust adaptive beamformer
Authors: Chen Xiangwei , Sheng Weixing 
Published: February 2023

Abstract
Eigen-subspace projection (ESP) is one of the most popular methods of robust adaptive beamforming, and the key issue of ESP is the selection of the projection subspace. The traditional ESP method treats the signal subspace as the projection subspace, so it needs source enumeration for a prior. Another inherent defect of it is its poor performance at low SNR. In this paper, we study in detail two methods for ESP-based robust adaptive beamforming. The main idea underlying these methods is to use the sequenced steering vector estimation to invert the estimate of the subspace dimension for an arranged eigenvector matrix. Since the proposed methods work without source enumeration, it is simple to implement. In addition, simulation results are included to illustrate the effectiveness and robustness of the proposed method as compared to the conventional ESP methods and other widely used robust techniques.
Like Moths Into the Fire: How Dark Triad Leaders can be Both Threatening and Fascinating
Authors: Diller Sandra , Czibor Andrea , Weber Magdalena , Klackl Johannes , Jonas Eva 
Published: February 2023

Abstract
The Dark Triad (DT; narcissism, Machiavellianism, psychopathy) has fascinated its audience in movies and theaters for decades. However, they also seem to evoke a sense of threat among people that interact with them. The present research hypothesizes that DT leaders are perceived as both threatening and fascinating. As fascination is approach-oriented while threat is avoidance-oriented, people facing a DT leader should be motivated to avoid and approach these leaders simultaneously, which could lead to a motivational conflict. In three studies (N1 = 255, N2 = 156, N3 = 138), we found that DT leaders evoked states of threat in the form of higher behavioral inhibition and lower behavioral activation. We further found that positive fascination emotions in terms of interest or curiosity were significantly higher in the control condition, while negative fascination emotions in terms of puzzlement or bafflement were substantially higher in the DT condition. The qualitative data gives additional insights into whata can be fascinating about a DT leader. Furthermore, the findings depict how both threat and fascination influence people's tendency for social proximity. Theoretical and practical implications are discussed.
Indicators and Factors Associated with Severe Maternal Outcomes at a Tertiary Health Facility in North-Eastern Tanzania: A Crossectional Study
Authors: Hassan Lisa , Kiritta Richard , Matovelo Dismas , Mujuni Fridolin , Hokororo Adolfine , Kihunrwa Albert , Ndaboine Edgar 
Published: February 2023

Abstract
Background: Severe maternal outcome is a complementary indicator of maternal health services. In absence of the patient’s testimony, reviews of maternal deaths alone often fail to provide critical details from the victim’s perspective. Combining maternal near misses and maternal deaths as two indicators for maternal health inform of severe maternal outcomes is designed to bridge this gap.
Methods: A crossectional study conducted between November 2021 and May 2022, included 287 women admitted with obstetric-related conditions. Sub-Saharan Africa (SSA) maternal near-miss tool was used to identify women with maternal near-miss. Descriptive statistics were used to evaluate obstetric characteristics and severe maternal outcome indicators. Factors associated with SMO were tested using chi-square and fishers exact test with a p-value <0.05 considered significant.
Results: A total of 287 women were enrolled in this study, from whom 175(60.9%) had severe maternal outcomes (SMO) in form of 138(78.9%) maternal near-miss and 37(21.1%) maternal deaths with a total of 3,071 recorded live birth. A calculated severe maternal outcome ratio of 56.9 per 1000 live births, a maternal near-miss ratio of 44.9 per 1000 live births, a mortality index of 0.21, and the maternal mortality ratio of 1204 per 100,000 live births were obtained. Referral from other health facilities {OR 6.1, 95% CI [2.38 – 16.0]} p-value <0.001, delaying for more than 30min to receive intervention at our facility {OR 6.3, 95% CI [1.16 – 34.6]} p-value 0.03 and admission in intensive care units/ high dependent units {OR 33[7.24 – 159.5]} p-value <0.001 were factors highly associated with an SMO event.
Conclusion: This is the first study of its kind across the northeastern zone of Tanzania. The above national average maternal mortality rate, a higher mortality index, a lower maternal near miss to maternal mortality ratio, and a high SMO ratio seen in this study indicates inadequacies in the health care system across different levels of care. Delay in receiving intervention for more than 30 minutes, referral from other health facilities, and admission to intensive care units are highly associated with a SMO event. SMO studies offer a better understanding of healthcare system challenges than studying maternal deaths alone.
Data mining of customer reviews to analyse the consumer experience in hospitals
Authors: Pandiya Bhartrihari , Singh Ranjit , Raj Aditya 
Published: February 2023

Abstract
Background
Consumer experience is crucial in the healthcare industry as customers need intensive care and attention. The digital review texts posted by the patients and their relatives can be a great tool to understand how the customers in the healthcare industry opine about different aspects of the services, facilities, and treatment provided in the hospitals. This paper attempts to analyze online customer reviews through data mining for understanding the experience of customers regarding different aspects of hospitals. The paper uses different text mining tools with part of speech-based tagging for aspect-based opinion mining. The analysis of the different aspects extracted from the review data shows that customers write reviews about the aspects of the hospitals such as doctors, staff, facilities, treatment, care, overall management etc. The perception towards the staff, facilities, services, and treatment also highly contributes to the positive review ratings and hence positive consumer experience. The research work provides insights to stakeholders such as healthcare professionals and hospital administration. The digital space and footprint of the hospitals should also be positive as it is viewed by prospective customers. Government should also have stringent policies for continuously low-rated hospitals.
Developing the CARE intervention to enhance ethical self-efficacy in dementia care through literary texts
Authors: Lauridsen Sigurd , Schou-Juul Frederik , Folker Anna Paldam , Phil Marie-Elisabeth , Simonsen Peter , Skov Sofie 
Published: February 2023

Abstract
Background
Dementia care is essential to promote the well-being of patients but remains a difficult task prone to ethical issues. These issues include questions like whether manipulating a person with dementia is ethically permissible if it promotes her best interest or how to engage with a person who is unwilling to recognize that she has dementia.
To help people living dementia and carers manage ethical issues in dementia care, we developed the CARE intervention. This is an intervention focused on promoting the ethical self-efficacy of people living with dementia and carers, i.e., their confidence that they can manage ethical issues when they occur.
The purpose this paper is to explain and discuss how we have developed the CARE intervention to promote the ethical self-efficacy of people living with dementia, their family, and professional carers through a specific and, we believe, new use of literary texts.
Methods
The CARE intervention has been developed in two phases: First, we conducted a needs assessment of the occurrence of ethical issues in dementia care and the need for an intervention to support people living with dementia and their carers in managing such issues. Second, in a design phase, we developed the CARE intervention to meet identified needs.
Results
To address identified ethical issues in dementia care we designed the CARE intervention as a workshop format where people living with dementia and carers can meet, discuss literary texts, and deliberate on how to solve such issues. The workshop is structured by the following elements: An agenda of ethical issues, a collection of literary cases exemplifying ethical issues, a moderator with an understanding of dementia care, and an overview of the ethical principles relevant to the discussion of ethical issues.
This workshop concept is operationalized in three applications tailored to meet the specific ethical issues of each of the study´s three target groups: People living with dementia and family carers, professional and family carers, and professional carers.
Conclusion
We conclude the paper by stating that it is possible to develop an intervention that promotes the ethical self-efficacy of people living with dementia and family and professional carers.
Impact of social disparities and screening attendance on cervical cancer incidence among Danish women, 1987-2016.
Authors: Bønløkke Sara , Blaakær Jan , Steiniche Torben , Iachina Maria 
Published: February 2023

Abstract
Background: For cervical cancer (CC), the implementation of preventive strategies has the potential to make CC occurrence and death largely avoidable. To better understand the factors possibly responsible for the barriers to HPV vaccination and screening, we aimed to examine possible differences in biological and social parameters as well as screening adherence between women with and women without a prior CC diagnosis and according to disease stage.
Methods: Through the Danish Cancer Registry (DCR), women diagnosed with CC in Denmark between 1987 and 2016 were included. These were age- and residence-matched in a 1:5 ratio with controls from the general female population. The study population was sub grouped into a low-stage subpopulation with women with early-stage CC and matched controls and a high-stage subpopulation with women with late-stage CC and matched controls. Biological and social parameters were compared within the subpopulations as well as between low- and high-stage cases. For part of the study population, screening attendance was examined to compare differences in adherence.
Results: Overall, we found that the risk of CC is significantly increased in socially disadvantaged women and not least non-attenders in screening. Interestingly, the high-stage subpopulation was significantly older than the low-stage subpopulation (p<0.001), and when examining the impact of age further, we found that the risk of getting low-stage CC decreases significantly with increasing age, whereas the risk of getting high-stage CC increases significantly with increasing age. In the screening cohort, significantly less cases than controls were attenders in screening with the most pronounced differences seen in the old subpopulation (women aged 50-64 years) and in the high-stage subpopulation (p-values all <0.001). Interestingly, when examining the risk of CC for attenders and non-attenders, we demonstrated that many social parameters continue to influence the risk of CC, even in women attending screening.
Conclusions:Older women, socially disadvantaged women, and non-attenders in screening are particularly vulnerable in terms of developing CC, especially high-stage disease. Therefore, improvements in the participating rate in screening as well as a revision of the current screening guidelines are needed.
The artemisinin-induced dormant stages of Plasmodium falciparum exhibit hallmarks of cellular senescence and drug resilience
Authors: Tripathi Jaishree , Stoklasa Michal , Nayak Sourav , Low Kay En , Lee Erica Qian Hui , Rénia Laurent , Malleret Benoît , Bozdech Zbynek 
Published: February 2023

Abstract

Recrudescent infections with human malaria parasite, Plasmodium falciparum, presented traditionally the major setback of artemisinin-based monotherapies. Although introduction of artemisinin combination therapies (ACT) largely solved the problem, the ability of artemisinin to induce dormant parasites still poses major obstacle for current as well as future malaria chemotherapeutics. Here, we developed a robust laboratory model for induction of dormant P. falciparum parasites and characterized their transcriptome, drug sensitivity profile and cellular ultrastructure. We show that P. falciparumdormancy requires a ~5-days maturation process during which the genome-wide gene expression pattern gradually transitions from the ring-like state to a highly unique form. The transcriptome of the mature dormant stage carries hallmarks of cellular senescence with downregulation of most cellular functions associated with growth and development, but upregulation of selected metabolic functions and DNA repair. Moreover, the P. falciparum dormant stage is considerably more resistant to essentially all antimalaria drugs compared to the fast-growing asexual stages. Finally, the unique cellular ultrastructure further suggests unique properties of this new developmental stage of the P. falciparum life cycle that should be taken into consideration by new malaria control strategies.
SNW domain containing protein 1 (SNW1), a novel insulin resistant gene induces hyperglycemia via inducing gluconeogenesis and downregulating glucose uptake in gestational diabetic mother
Authors: BHUSHAN Ravi , Rani Anjali , Trivedi Rhimjhim , Gupta Dinesh Kumar , Tripathi Anima , Kumar Ajay , Rawat Shiv Govind , Dubey Pawan Kumar 
Published: February 2023

Abstract
Objective
Gestational Diabetes mellitus (GDM) is an emerging maternal health problem with increasing incidences. The lack of complete understanding of pathophysiological mechanism and novel regulatory biomarkers makes the early diagnosis difficult. Hence, the present study aims to decipher novel regulatory gene, which will help better understand the pathophysiology of GDM and serve as novel diagnostic and therapeutic biomarkers.
Materials and Methods
We performed high-throughput RNA sequencing on the Oxford Nanopore-MinION sequencing platform. Gene network analysis (STRING and Cytoscape), module extraction (MCODE), functional and pathway enrichment analysis (DAVID and Enrichr) were performed to identify the novel hub gene. Identified novel hub genes were validated through qRT-PCR and tested for their sensitivity and specificity. Serum metabolites were measured using 800MHz Bruker Avance NMR spectrometer and were compared between GDM and healthy control. PANC1 cells were transfected to validate the novel biomarkerand their possible regulatory mechanism. All expression and statistical analysis were carried out using Graph pad prism 9.
Results
Transcriptomic study leads to identification ofSNW1 as most sensitive and specific (AUC = 0.98; sensitivity = 96.7 and; specificity = 96.7; p < 0.001) biomarkerwhich isup-regulated (fold change = 1.09; p < 0.001) in GDM. Metabolomics study identified elevated gluconeogenesis which is evident from decreased level of amino acids like alanine (GDM = 397.5 ± 28.12; NGT = 515.1 ± 24.03) and elevated level of pyruvate (GDM = 17.35; NGT = 16.17) and glucose (GDM = 4.67; NGT = 3.25) in GDM and control respectively.SiRNA mediated knockdown of SNW1 causes significant downregulation of alanine aminotransferase (ALT/GPT), and insulin receptor substrate (IRS1) while significant upregulation of glucose transporter (GLUT2 and GLUT4) and insulin (INS) at mRNA as well as protein level both.
Conclusion
SNW1 is novel insulin resistant gene which induces hyperglycaemia via elevating gluconeogenesis and decreasing the glucose uptake. Hence SNW1 can be used as diagnostic biomarker for early diagnosis of GDM mother.
Prevalence and characterization of virulence-associated genes and antimicrobial resistance in Aeromonas hydrophila from freshwater finfish farms in Andhra Pradesh, India
Authors: Suresh Kummari , Pillai Devika 
Published: February 2023

Abstract

Aeromonas hydrophila, a natural inhabitant of aquatic environments and known to become pathogenic in fishes under favourable conditions, have also been reported to cause broad spectrum of infections in humans and animals. The prevalence, virulence- associated genes and antimicrobial resistance in 57 A. hydrophila isolates from 100 freshwater fish farms (253 fishes) of Andhra Pradesh, India was studied. The isolates showed resistance to trimethoprim (35.9%), co-trimoxazole (21.1%), oxytetracycline (19.3%), doxycycline (17.5%), and enrofloxacin (8.93%), while they were completely sensitive to ciprofloxacin. Majority of the A. hydrophila isolates were strong (33%), moderate (50%) and weak (17%) biofilm formers, having role in virulence and colonization in host. The virulence gene distribution in representative A. hydrophila isolates was very high for enolase (enol, 96.4%), followed by flagellin (fla, 94.7%), cytotonic enterotoxins (alt, 91.2%), cytotoxic enterotoxins (act, 84.2%), lipase (lip, 77.1%), serine protease (ahp, 54.3%), aerolysin (aerA, 50.8%), and elastase (ahyB, 43.8%), respectively. 80% of the isolates carried a maximum of four virulence genes, whereas none carried the DNAase (exu) or haemolysin (hly) genes. Hemolytic assays on blood agar demonstrated that β-hemolysis was present in 70% of the isolates. These findings will create awareness among aquaculturists on the potential risk of spread of the isolates and the virulence associated genes across other sectors and the need for practicing best management practices in aquaculture.