bluuebunny/crossref_metadata_2025_split huggingface dataset:
Dietary Practices And Perceptions During Pregnancy And Lactation Among Women In Rural Sindhudurg: A Qualitative Study
Authors: Deshpande Sonal , Potdar Pallavi 
Published: January 2025

Various regions of Maharashtra have different cultural practices. Pregnancy, childbirth, and lactation are circumstances where cultural beliefs strongly affect women's dietary practices. A qualitative study was conducted to describe dietary practices and perceptions during pregnancy and lactation among women in rural Sindhudurg. A cross-sectional study was conducted among pregnant and lactating women, women involved in cooking for their families, and frontline workers at the rural field practice area of SSPM Medical College, Sindhudurg. A homogenous group for Focused group discussions and a typical case method for In-depth Interviews were used. The mean age of the total 66 participants was 49.2 + 12.3 years. All the pregnant women agreed that their diet should include fruits, sprouts, and milk. There was a special diet for lactating women, especially during puerperium. Methi laddu, dink ladoo, and dry fruits laddu were consumed by lactating women as galactagogues. They had various myths regarding foods to be avoided during pregnancy. Lactating mothers believed that their diet does affect breast milk composition. There were myths regarding foods that, when consumed by lactating women, may lead to specific symptoms or conditions in babies. The Mean dietary diversity scores of pregnant and lactating mothers were low and were lowest during puerperium. Though many pregnant women know nutritious food, affordability and time availability are constraints. Dietary restrictions, especially during the early postpartum period, led to less dietary diversity. Some of the beliefs are deep-rooted and need to be more easily amenable.
Keywords: Diet, lactation, pregnancy, qualitative, rural Sindhudurg
The Role of Environmental Sanitation and Personal Hygiene in Soil Transmitted Helminths Infection in School-Age Children in Banjarharjo Sub-district
Authors: EKANIASARI MEVIANA DEA , WARDANI DITA PRATIWI KUSUMA , MUJAHID IKHSAN , ALMANFALUTHI MUHAMMAD LUTHFI 
Published: January 2025

Helminthiasis in school-age children can impede both physical and cognitive development. Poor personal hygiene practices are associated with various health issues among schoolchildren, including diarrhoea and helminth infections. A healthy environment is crucial for physical and mental well-being, whereas poor sanitation significantly increases the risk of infectious diseases. This study aims to examine the relationship between environmental sanitation, personal hygiene, and soil-transmitted helminth infections among school-age children in the Banjarharjo Sub-district. This analytic observational study employed a cross-sectional design and was conducted in July 2023. Data collection involved administering a personal hygiene and environmental sanitation questionnaire, alongside identifying soil-transmitted helminth (STH) eggs in faeces and nail samples using the MgSO4 sedimentation method. Participants were selected through purposive sampling based on predefined inclusion and exclusion criteria. The study population comprised school-age children from Banjarharjo District, with a final sample size of 58 children from Sindangheula Village and Cikuya Village. Data analysis was performed using the Fisher exact test. The results showed no association between environmental sanitation (faeces p = 0.583; nails p = 1.00) and personal hygiene (faeces p = 1.00; nails p = 1.00) and soil-transmitted helminth infection in both faeces and nail samples (p > 0.05). Therefore, the study found no significant relationship between environmental sanitation, personal hygiene, and soil-transmitted helminth infection among school-age children in Banjarharjo Sub-district.
Keywords: Environmental sanitation, personal hygiene, school age, soil-transmitted helminths
Safety Goggles Myopi as a Worker Protector with Myopic Eye Complaints
Authors: Naufal Ilham Saputra  , Ramadhani Husnul Kirom , Violin Margaretha Puspita Ningrum  , Amrina Rosyadah  , Muslikha Nourma Rhomadhoni  
Published: January 2025

ABSTRACT

The number of workers who have near-sightnedness and is supported by a lack of compliance in use of PPE for comfort reasons causes the potential for work accidents. This research aimed to reduce the number of eye injuries and become an innovation in safety glasses and PPE for workers complaints of myopia. This study used an analytical descriptive method with a cross-sectional study design, which was carried out from June to October 2022. The total respondents in this study were 60 respondents. Furthermore, this study used an instrument, namely a survey questionnaire through a comprehensive Google Form. From the survey, 55% of respondents used glasses, 53.3% of respondents experienced complaints of myopia, 18.3% of respondents have worn glasses before work, 20% of respondents were required to wear protective glasses while working, and 80% of respondents showed interest in "Safety Goggles Myopi". The test results at the laboratory of Nahdlatul Ulama University Surabaya using the Lutron Type-340A UV Metershowed that "Safety Goggles Myopi" is effective in reducing exposure to UV radiation from the sun and welding rays to 0 mW/m2. Additionally, "Safety Goggles Myopi" also has a concept of glasses with 3 in 1 lenses that are comfortable and practical when used. Through these results, the idea of PPE innovation entitled "Safety Goggles Myopi" is appropriate to reduce the number of work accidents, increase compliance in the use of PPE protective glasses in the workplace, especially for workers who have near-sightedness, and improve the implementation of K3’s culture in Indonesia.
Keywords: Eye complaints, K3 culture, near-sightedness, PPE Use, safety goggles myopi, workers, work accidents.
Stress Level Overviews of Final Semester Students in Faculty of Health Universitas Nahdlatul Ulama Surabaya
Authors: Rosyadah Amrina , Firmansyah Dio , Hardiastuty Hanny Elok , Fauziah Khusniada Inayatul , Sunaryo Merry , Rosyadah Amrina 
Published: January 2025

Stress is a condition that can be caused by uncontrolled physical, environmental, and social demands. Stress can come from external factors originating from the environment or caused by the individual's internal perception. Stress in students can also be caused by stress in the form of parental demands, the complexity of the courses used in the thesis, a bad environment, conflict with friends, meeting supervisors, and other people. This research provides an overview of the stress level in final-semester students at the Faculty of Health, Universitas Nahdlatul Ulama Surabaya. The research design used in this research is pre-experimental The variables used are a quantitative approach that focuses on one variable, namely stress levels. The research results showed that respondents experienced severe stress with a percentage of (71%) and those who experienced mild stress were (29%). This study used five indicators of stress sources including lack of understanding of the topic of 8 students (11%). This economic factor indicator is one of the factors widely experienced by students with a total of 28 students (39%), in comparison concerns about not being able to take the exam 27 students (37%). In addition, two other indicators, namely family and friend support as many as 7 students (10%) and 2 students (3%) came from supervisors.
Keywords : Final-semester, student, indicator, stress
ANALYSIS OF INFLUENCING FACTORS BAZNAS HUMAN RESOURCES PLANNING (HRP) MODEL
Authors: Hadi Moch. Aminudin , Kurniawan Robby 
Published: January 2025

The purpose of this qualitative research is to obtain factors that affect the human resources planning (HRP) of the National Amil Zakat Agency (BAZNAS) and the important themes that are in it. The establishment of BAZNAS as the main organization for zakat management in Indonesia has been a long journey. Human resources management of BAZNAS is very important in supporting BAZNAS' role in Indonesia. This research uses qualitative paradigm and with phenomenology approach strategy. Data collection techniques were conducted by interviewing 8 informants. The interview process was recorded by audio recording device and transcribed. The transcription result were arranged in tabulation based on some specified themes. The model was built with Interpretative Structural Modelling (ISM) approach and supported by Strategic Assumption Surfacing and Testing (SAST) method. The result of this research found 10 elements that build the BAZNAS Human Resources planning model, namely: 1) HR strategic plan, 2) HR planning and procurement, 3) leadership role, 4) HR audit, 5) sharia criteria, 6) digital friendly criteria, 7) key withdrawal and selection system, 8) special work standards & certification, 9) leadership competency and supporting certification, and 10) building organizational culture. The contribution of this research is to provide a theoretical basis for preparing BAZNAS HR planning throughout Indonesia.
Pengembangan Urban Nutrigarden Sebagai Pemberdayaan Ekonomi Masyarakat Dengan Menggunakan Internet Of Things (Iot)
Authors: Nurmatiani Erika , Ryanto Muhamad 
Published: January 2025

Urban Nutrigarden adalah kegiatan bertanam sayuran dan herba secara organik di lingkungan perkotaan. Konsep Urban Nutrigarden memanfaatkan ruang yang tersedia di rumah, mulai dari pekarangan, vertikal, atap rumah, hingga tanah berbatu. Konsep Urban Nutrigarden yang ada pada komunitas ini berkonsep ramah lingkungan dan berkelanjutan. Adapun tujuan budidaya adalah untuk mendapatkan variasi nutrisi seimbang, membentuk ekosistem yang berkelanjutan di dalam rumah serta dapat meningkatkan perbaikan ekonomi Mendorong mitra budidaya Microgreens untuk terus mengimplementasikan penerapan teknologi IoT terhadap konsep Nutrigarden dalam kegiatan budidaya mereka secara mandiri.
Jurnal Pendampingan Pengolahan dan Pembuatan Teh Kombucha Penuh Manfaat Bagi Kesehatan Terhadap Pemuda-Pemudi Majlis Taklim Ar-Rahman Kabupaten Garut
Authors: Amirudin Jafar , Komariah Iis , Ginanjar Ani Yanti , Putri Megawati 
Published: January 2025

Tujuan dari pengabdian ini adalah pendampingan terhadap komunitas pemuda-pemudi, dengan banyaknya manfaat yang ddapatkan dari Teh Kombucha di harapkan ini akan menjadi oleh-oleh khas garut yang dapat di minati oleh masyarakat banyak, juga sebagai minuman alternative untuk mengobati beragam gangguan kesehatan dimasyarakat, sehingga hal ini akan membuka lapangan kerja baru bagi masyarakat. Pelaksanaan kegiatan ini dilaksanakan di Majlis Taklim ar-Rahman di Sukaresmi Garut dengan jumlah peserta sebanyak 30 orang. Metode yang digunakan dalam pengabdian ini adalah Participatory Action Research (PAR) yaitu memiliki arti pelaku pengabdian masyarakat merasakan langsung pendampingan pembuatan teh kombucha dilapangan dengan masyarakat. Ada empat tema dasar dalam PAR, yaitu kolaborasi melalui partisipasi, mendapat pengetahuan, dan perubahan sosial. Hasil dari pendampingan pembuatan teh kombucha ini pembuatan teh dari bibit kombucha yang sudah tersedia, kemudian melakukan pelatihan dari seorang ahli kombucha yang memiliki sertifikat dan dilakukan pendampingan pembuatan teh kombucha serta pelatihan pengemasan dan strategi usaha untuk memasarkan produk yang telah dihasilkan.
Whole-genome sequencing reveals transmission pattern and drug resistance of Mycobacterium tuberculosis intra- or inter-hosts
Authors: Ding Feng , Liu Wanfei , Wu Chi , Zhang Wensi , Chen Shuyan , Lai Wenjie , Qu Jiayao , Lin Qiang , Lu Shuihua , Qu Jiuxin 
Published: January 2025

BackgroundTuberculosis (TB) remains a serious global public health problem. The Mycobacterium tuberculosis (MTB) is responsible for approximately 10 million new TB cases globally each year. This study aimed to investigate transmission pattern and drug resistance of MTB in Shenzhen, China.MethodsA retrospective study on 286 samples from 184 TB patients collected between 2015 and 2018 in Shenzhen Third People’s Hospital was conducted using whole-genome sequencing. Drug susceptibility testing (DST) was performed using both phenotypic DST (pDST) and molecular DST (mDST). Sample diversity was evaluated by SNPs and transmission clusters were identified based on SNP differences of 12 or fewer in genetic clusters.ResultsExcept four samples identified as non-tuberculous mycobacteria, 282 MTB samples (181 patients) underwent mDST, with 244 samples (162 patients) undergoing pDST. The overall multidrug-resistant rate in patients was 22.31% in pDST (12.00% for new patients and 40.82% for retreatment patients) and 34.48% in mDST (20.41% for new patients and 58.21% for retreatment patients). Totally 92 transmission clusters were identified, encompassing 70.21% samples (57.46% patients), with 5 clusters containing samples (15, 5.32%) from different patients (9, 4.97%), indicating recent transmission. The drug-resistant mutations in 36 of 45 transmission clusters (80.00%) were identical in all samples, suggesting the transmission of drug resistance. Patients with multiple samples were categorized into simultaneous sampling (SS) and continuous sampling (CS) groups, revealing significant differences in treatment types, treatment outcomes, residential addresses, and drug resistance types. mDST showed greater accuracy than pDST in SS and CS groups. A novel method based on heterozygous SNPs and two-sample Kolmogorov–Smirnov test were developed and identified 12 (4.26%) samples as mixed infection samples. Six of 12 patients had mixed and pure samples together, and major strains of mixed samples were closer to corresponding pure strains than minor strains.ConclusionsThis retrospective study, conducted at the only municipal hospital specializing in infectious diseases in Shenzhen, provides the opportunity to understand drug resistance of TB patients, which mainly are refractory patients. The study revealed transmission patterns of MTB, analyzed mixed infections, and tracked changes in MTB strains during short/long-term treatment.
Characterization of the Vibrio anguillarum VaRyhB regulon and role in pathogenesis
Authors: Li Yingjie , Yu Xinran , Li Peng , Li Xin , Wang Lushan 
Published: January 2025

BackgroundThe marine Gram-negative bacterium Vibrio anguillarum is one of the major pathogens in aquaculture. Iron uptake is a prerequisite for virulence and is strictly controlled by a global iron uptake regulator, Fur, which acts as a repressor under iron-replete conditions. When iron is depleted, Fur also functions as an activator, playing an important role in pathogenesis. It is unclear whether this upregulation model is mediated by a small RNA, RyhB. MethodsThe small RNA, VaryhB, was deleted in V. anguillarum strain 775, and its regulon was investigated using transcriptomic analysis. The roles of VaRyhB in siderophore synthesis, chemotaxis and motility, and oxidative stress were evaluated using chrome azurol S (CAS) liquid assay, swimming motility assay, and intracellular reactive oxygen species (ROS) assay, respectively. The virulence of VaRyhB was evaluated by challenging turbot larvae intraperitoneally. ResultsThe small RNA called VaRyhB identified in V. anguillarum strain 775 is significantly longer than that in Escherichia coli. Transcriptomic analysis revealed that VaRyhB is critical for iron homeostasis under limited iron conditions, and deletion of VaRyhB resulted in lower expression levels of certain genes for siderophore biosynthesis and transport, thereby leading to impaired growth, reduced siderophore production, and decreased pathogenesis. The virulence factor motility is also upregulated by VaRyhB, and reduced motility capability was observed in the ΔVaryhB mutant, which may be another reason resulting in weak pathogenesis. The sensitivity toward H2O2 in the ΔVafur mutant could be restored by the loss of VaRyhB, suggesting that the role of Fur in oxidative stress is mediated by VaRyhB. VaRyhB also functions to inhibit the expression of genes involved in Fe-S assembly and the TCA cycle. In addition, two aspects of the type VI secretion system and molybdenum cofactor biosynthesis were first identified as being regulated by VaRyhB. ConclusionIn V. anguillarum, the sRNA VaRyhB plays a critical role in the inhibition of genes involved in the TCA cycle, Fe-S assembly, and the type VI secretion system. It is also essential for the activation of siderophore synthesis, chemotaxis and motility, and anaerobic denitrification. Our work provides the first evidence of the VaRyhB regulon and its role in the pathogenesis of V. anguillarum.
PCSK9-antibodies fail to block PCSK9-induced inflammation in macrophages and cannot recapitulate protective effects of PCSK9-deficiency in experimental myocardial infarction
Authors: Rauterberg Simon , Härdtner Carmen , Hein Jennifer , Schrepf Paola , Peyronnet Remi , Koentges Christoph , Vico Tamara A. , Ehlert Carolin , Dufner Bianca , Lindner Diana , von zur Mühlen Constantin , Wolf Dennis , Westermann Dirk , Hilgendorf Ingo , von Ehr Alexander 
Published: January 2025

Background and aimsProprotein convertase subtilisin/kexin type 9 (PCSK9) plays a crucial role in cholesterol homeostasis by regulating low-density lipoprotein (LDL) receptor levels. Despite its known effects on cholesterol metabolism, the role of PCSK9 in cardiac function, especially post-myocardial infarction (MI), remains unclear. This study investigates the impact of PCSK9 on heart function post-MI and evaluates the effects of PCSK9 inhibition via Alirocumab.MethodsWe used PCSK9 knockout (KO) mice and wildtype (WT) mice and in vivo treatment with Alirocumab to analyze cardiac function and survival post-MI induced by permanent ligation of the left anterior descending artery. PCSK9 and LDL receptor levels were measured using ELISA and qRT-PCR. Cardiac function was assessed via echocardiography and isolated working heart model experiments. Gene expression changes were evaluated using RNA sequencing, and inflammatory responses in bone marrow-derived macrophages (BMDMs) were analyzed in vitro.ResultsPCSK9 was expressed in murine heart tissue at levels comparable to the liver, despite minimal heart RNA expression. PCSK9 KO mice had lower plasma cholesterol levels and showed reduced cardiac functions in the working heart model compared to WT mice. Post-MI, PCSK9 KO mice demonstrated significantly improved survival and reduced ventricular rupture compared to WT mice. Alirocumab treatment, while effective in lowering plasma cholesterol, did not replicate the survival benefits seen in PCSK9 KO mice and even worsened cardiac function post-MI. In vitro, PCSK9 induced significant inflammatory responses in macrophages, which were not mitigated by Alirocumab.ConclusionPCSK9 accumulation in the heart post-MI contributes to adverse cardiac remodeling and inflammation. Genetic deletion of PCSK9 confers protection against post-infarct mortality, whereas pharmacological inhibition with Alirocumab fails to reproduce these benefits and may exacerbate cardiac dysfunction. These findings highlight the complex role of PCSK9 in cardiac pathology and caution against the assumption that PCSK9 inhibitors will necessarily yield cardiovascular benefits similar to genetic PCSK9 deficiency.
Integrating general practitioners’ and patients’ perspectives in the development of a digital tool supporting primary care for older patients with multimorbidity: a focus group study
Authors: Schäfer Ingmar , Jahns Vivienne , Paucke Valentina , Lühmann Dagmar , Scherer Martin , Nothacker Julia 
Published: January 2025

IntroductionThe web application gp-multitool.de is based on the German clinical practice guideline “multimorbidity” and supports mutual prioritisation of treatments by GPs (general practitioners) and patients. The application facilitates sending hyperlinks to standardized assessments by email, which can be completed by patients on any suitable digital device. GPs can document clinical decisions. The tool also supports a structured medication review. Aims of this study were to consider needs and wants of the target groups in implementing the “multimorbidity” clinical practice guideline in a digital tool, and to examine themes of discussions in order to identify which aspects were considered most important for customising a digital tool.Materials and methodsWe conducted six focus groups with 32 GPs and six focus groups with 33 patients. Eight groups were conducted alongside the programming of the web application and four after finishing a prototype. GPs were recruited by mail and asked to invite up to six eligible patients from their practice to participate. Focus groups were based on semi-structured interview guides and discussed assessments, functionalities, usability and reliability of gp-multitool.de. Discussions were transcribed verbatim and analysed using content analysis.ResultsGPs wanted to avoid unnecessary and time-consuming functions and did not want to explore problems that they could not provide solutions for. For some assessments, GPs suggested simplifying scales or including residual categories. GPs and patients also addressed possible misunderstandings due to wording and discussed if some items might be too intimate or overtax patients intellectually. In most cases, participants confirmed usability, but they suggested changes in default settings and pointed out a few minor bugs that needed to be fixed. While some GPs considered data security an important topic, most patients were unconcerned with this issue and open to share their data.ConclusionOur study indicates that focus groups can be used to customize a digital tool according to the needs and wants of target groups and thus, improve content, functionality, usability, and reliability of digital tools. However, digital tools still need to be piloted and evaluated in everyday care. In our focus groups, study participants confirmed that gp-multitool.de can be a relevant approach for overcoming deficits in the information needed for mutual prioritisation of treatments by GPs and patients.
Towards a green world: how new urbanization affects green total factor carbon productivity
Authors: Wang Xiaohong , Li Xuanting 
Published: January 2025

IntroductionNew urbanization is a powerful support for regional economic growth and green transformation.MethodsIn this paper, we select data from 237 cities at prefecture level and above in China from 2010 to 2021 for quasi-experimental design, and investigate the effects of new urbanization policy pilots on urban green total factor productivity (GTFP) and its mechanism by constructing a multi-period difference-in-differences model.ResultsThe study finds that: a) the construction of new urbanization has a positive impact on promoting urban GTFP, and this result is verified by a series of robustness tests; b) the promotion of industrial structure upgrading, the facilitation of green technological innovation, and the reduction of the degree of resource mismatch all partially mediate the relationship between new urbanization and urban GTFP; c) environmental regulation and market integration both modulate the relationship between the construction of new urbanization and urban GTFP in positive directions; d) heterogeneity analysis shows that, in terms of the two-dimensional city attributes of ‘location-characteristics’, the increase in GTFP is more significant in eastern and central cities, and in non-resource cities; e) further analysis shows that the pilot policies of new urbanization have a spatial spillover effect on GTFP, and the policy spillover effects are mainly affected through mechanisms such as industrial structure upgrading in neighboring municipalities.DiscussionThis study provides new ideas for the next stage of sustainable regional green development in China, thus promoting China's high-quality development with theoretical and practical significance.