bluuebunny/crossref_metadata_2025_split huggingface dataset:
Nitrate-responsive transcriptome analysis of the rice RGA1 mutant reveals G-protein α subunit regulation of nitrogen-sensitivity and -use efficiency
Authors: Prasanna Jangam Annie , Mandal Vikas Kumar , Kumar Dinesh , Chakraborty Navjyoti , Raghuram Nandula 
Published: August 2023

Abstract
Nitrogen (N) use efficiency (NUE) is important for sustainable agriculture. G-protein signalling was implicated in N-response/NUE in rice, but needs genetic characterization of the role of α subunit (RGA1). We found that RGA1 knock-out mutant in japonica rice exhibited lesser nitrate-dose sensitivity, higher yield and NUE relative to the wild type (WT). We investigated its genomewide basis using nitrate-responsive rga1 transcriptome relative to WT. It revealed 3,416 differentially expressed genes (DEGs), including 719 associated with development, grain yield and phenotypic traits for NUE. The upregulated DEGs were related to photosynthesis, chlorophyll, tetrapyrrole and porphyrin biosynthesis, while the downregulated DEGs belonged to cellular protein metabolism and transport, small GTPase signalling, cell redox homeostasis etc. RT-qPCR validated 26 nitrate-responsive DEGs across functional categories. Physiological validation of nitrate-response in the mutant and WT at 1.5 and 15 mM doses revealed higher chlorophyll and stomatal length but decreased stomatal density, conductance and transpiration. The consequent increase in photosynthesis and water use efficiency may contribute to higher yield and NUE in the mutant, whereas the WT was N-dose sensitive. The mutant was not as N dose-responsive as WT in shoot/root growth, productive tillers and heading date, but equally responsive as WT in total N and protein content. RGA1mutant is less impacted by higher N-dose or salt stress in terms of yield, protein content, photosynthetic performance, relative water content, water use efficiency and catalase activity. PPI-network analyses revealed known NUE-related proteins as RGA1 interactors. Therefore, RGA1 regulates N-dose sensitivity and NUE in rice.
Insight into synthesis and characterisation of Ga0.9Fe2.1O4 superparamagnetic NPs for biomedical applications
Authors: Mesaros Amalia , Garzón Alba , Nasui Mircea , Bortnic Rares , Vasile Bogdan , Vasile Otilia , Iordache Florin , Leostean Cristian , Ciontea Lelia , Ros Josep , Pana Ovidiu 
Published: August 2023

Abstract
A Ga3+-substituted spinel magnetite nanoparticles (NPs) with the formula Ga0.9Fe2.1O4 were synthesized using both the one-pot solvothermal decomposition method (TD) and the microwave-assisted heating method (MW). Stable colloidal solutions were obtained by using triethylene glycol, which served as a NPs stabilizer and as a reaction medium in both methods. A narrow size cridistribution of NPs, below 10 nm, was achieved through selected nucleation and growth. The composition, structure, morphology, and magnetic properties of the NPs were investigated using FTIR spectroscopy, thermal analysis (TA), X-ray diffraction (XRD), transmission electron microscopy (TEM), X-ray photoelectron spectroscopy (XPS), and magnetic measurements. NPs with the expected spinel structure were obtained in the case of the TD method, while the MW method produced, additionally, an important amount of gallium suboxide. The NPs, especially those prepared by TD, have superparamagnetic behavior. In the case of MW preparation, the presence of a secondary phase affects both the dimensional distribution and the magnetic behavior of NPs. The NPs were tested in vitro on amniotic mesenchymal stem cells. It was shown that the cellular metabolism is active in the presence of Ga0.9Fe2.1O4 NPs and also preserves an active biocompatible cytoskeleton.
Genome-wide identification of WRKY gene family and expression analysis under abiotic stresses in Avena sativa
Authors: Ren Guoling , Li Mingjing , Han Zihui , Zhou Jiahui , Yin Yajie , Chen Naiyu , Ling Lei 
Published: August 2023

Abstract
The WRKY gene family plays a vital role in plant growth and development and abiotic stress response. This will provide reference for further study on the regulation mechanism of WRKY gene family in response to abiotic stress in A.sativa. In this study, We identified 165 WRKY family members by bioinformatics methods. Furthermore, the chromosomal localization, phylogenesis, conserved motifs, gene structure and gene replication were analyzed for WRKY genes. There are many response elements related to abiotic stress in AsWRKY member promoter region. qRT-PCR experimental results showed that WRKY gene played a regulatory role in cold stress, low temperature, salt stress and salt-alkali stress. This study provided theoretical basis for further exploring the biological functions of WRKY gene family members in A.sativa.
Neck stiffness and bone osteolytic lesion in infants: a case report
Authors: Carraro Carolina , Rabusin Marco , Murru Flora Maria , Ammar Lydie , Barbi Egidio , Amaddeo Alessandro , Cozzi Giorgio 
Published: August 2023

Abstract
Background Neuroblastoma is the most frequent extracranial solid tumor occurring in childhood, representing approximately 28% of all cancers diagnosed in infants. Signs and symptoms of neuroblastoma vary with the site of development of the tumor and can mimic other diseases due to its extreme clinical variability. However, torticollis is not reported in the medical literature as a leading symptom of neuroblastoma.
Case presentation Here we report the case of a 3 years-old girl with fever and neck stiffness. Blood tests revealed a mild anemia and a rise in inflammatory markers. CT-scan showed a solid, heterogeneous, predominantly hypodense surrenal mass with eccentric calcification and extensive inhomogeneity of the vertebral metamers. A course of chemotherapy for neuroblastoma was promptly started with immediate clinical improvement.
Conclusions This case shows that the presence of torticollis could be a chief complaint of neuroblastoma. To our knowledge, neuroblastoma is not mentioned among life-threatening underlying conditions of torticollis in most recent literature reviews.
This case advises general practitioners that in presence of unexplained torticollis in a toddler a neuroblastoma should be ruled out.
Viral Communities in Millipede Guts: Insights into Diversity and the Potential Role in Modulating the Microbiome
Authors: Nweze Julius Eyiuche , Schweichhart Johannes Sergej , Angel Roey 
Published: August 2023

Abstract

Millipedes are important detritivores harbouring a diverse microbiome. Previous research focused on the microbiome, while the virome remains neglected. We elucidated the viral diversity in the hindguts of two millipede model species with distinct microbiomes: the tropical Epibolus pulchripes and the temperate Glomeris connexa. Based on metagenomic and metatranscriptomic assembled viral genomes (MAVGs), the viral communities differed markedly and preferentially infected the most abundant prokaryotic taxa. The majority of DNA viruses were Caudoviricetes (dsDNA), Cirlivirales (ssDNA), and Microviridae (ssDNA), while RNA viruses consisted of Leviviricetes (ssRNA), Potyviridae (ssRNA) and Eukaryotic viruses. A high abundance of subtypes I-C, I-B and II-C CRISPR-Cas systems was found, primarily from Pseudomonadota, Bacteroidota and Bacillota. In addition, auxiliary metabolic genes (AMGs) that modulate chitin degradation, vitamins and amino acid biosynthesis, and sulfur metabolism were also detected. Lastly, we found low virus-to-microbe-ratios (mVMR) and a prevalence of lysogenic viruses, supporting a Piggyback-the-Winner dynamic in both hosts.
Computing all persistent subspaces of a reaction-diffusion system
Authors: Peter Stephan , Woitke Linus , Dittrich Peter , Ibrahim Bashar 
Published: August 2023

Abstract
An algorithm is presented for computing for a reaction-diffusion partial differential equation (PDE) system all possible subspaces that can hold a persistent solution of the equation. For this, all possible sub-networks of the underlying reaction network that are distributed organizations (DOs) are identified, because recently it has been shown that a persistent subspace must be a DO. The algorithm computes the hierarchy of DOs starting from the largest by a linear programming (LP) approach using integer cuts. The underlying constraints use elementary reaction closures (ERCs) as minimal building blocks to guarantee local closedness and global self-maintenance, required for a DO. Additionally, the algorithm delivers for each subspace an affiliated set of organizational reactions (SOR) and a minimal compartmentalization that is necessary for this subspace to persist. It is proved that all SORs of a reaction network, as already DOs, form a lattice and that this lattice contains all potentially persistent sets of reactions of all constrained solutions of reaction-diffusion PDEs. This provides a hierarchical structure of all persistent subspaces with regard to the species and also to the reactions of the reaction-diffusion PDE system. Here, the algorithm is described and corresponding python source code is provided. Furthermore, an analysis of its run time is performed and all models from the BioModels database as well as further examples are examined. Apart from the practical implications of the algorithm the results also give insights into the complexity of solving reaction-diffusion PDEs.
Comparative Investigation of The Effects of Conventional and Skeletal Anchorage Supported Pendulum Appliances on Dentofacial Structures
Authors: Aksoy Ayca , Ozen Oyku , Erdem Murat Kaan , Erdem Dilek 
Published: August 2023

Abstract
Objective: The aim of this study is to compare the effects of the conventional pendulum and pendulum appliances applied with miniscrew to eliminate the anchorage loss on skeletal and dentoalveolar structures.
Materials and Methods: Research groups consist of 30 individuals with skeletal class 1, dental class II malocclusion. The patients included into 3 groups. Group 1 who treated with conventional pendulum appliance; Group 2 who are applied pendulum with single miniscrew; Group 3 who are applied pendulum with double miniscrews. Pendulum appliances were completed when Class I molar relationship was obtained and data were analysed.
Results: Distalization applications was statistically significantly shorter in Group 3. Distal tipping was observed in all groups. In incisor teeth, protrusion was observed in both groups except the Group 3. The increase in the overjet was found to be statistically significantly lower in Group 3. Changes in SNA values were not statistically significant, the decrease in the SNB value was insignificant in Group 1, while a statistically significant decrease was observed in the groups using skeletal anchorage.
Conclusion: Ideal molar relationship has been achieved in all pendulum appliances without patient cooperation. Side effects were seen less in pendulum which skeletal anchoring was provided with double miniscrew support and treatment time in this was shortened.
Clinical Relevance: Pendulum appliances have specific uses in orthodontics. When these appliances are used in the proper indication, they shorten the treatment time and increase patient motivation. Clinicians using pendulum should be know their clinical indications, the potential problems and benefits.
Prevalent Landscape of Tumor Genomic Alterations of Luminal B1 Breast Cancers Using a Comprehensive Genomic Profiling Assay in Taiwan
Authors: Chen Bo-Fang , Tsai Yi-Fang , Lien Pei-Ju , Lin Yen-Shu , Feng Chin-Jung , Chen Yen-Jen , Cheng Han-Fang , Liu Chun-Yu , Chao Ta-Chung , Tseng Ling-Ming , Huang Chi-Cheng 
Published: August 2023

Abstract
Background:
The human epidermal growth factor receptor 2 (HER2) negative luminal B1 subtype of breast cancer has been reported with a poorer outcome than luminal A in recent studies. This study aimed to investigate the molecular alterations and identify potential therapeutic targets by analyzing the genetic profiling from a cohort of luminal B1 breast cancer in Taiwan.
Methods:
We enrolled patients with luminal B1 breast cancer in our study. They were classified as patients who received curative surgery and adjuvant or neoadjuvant chemotherapy as the low-risk group, and who had advanced or metastatic disease or early relapse during the follow-up time as the high-risk group. Using targeted sequencing, we evaluated genomic alterations, interpreting variants with the ESMO Scale of clinical actionability of molecular targets (ESCAT).
Results:
A total of 305 lumina B1 breast cancer patients underwent targeted sequencing analyses. The high-risk patients reported more actionable genes and called variants than the low-risk group (P < 0.05). PIK3CA (42%), FGFR1 (25%) and BRCA1/2 (10.5%) were the most prevalent ESCAT actionable alterations in luminal B1 breast cancer. There was no difference in the prevalence of actionable mutations between these two groups, except for ERBB2 oncogenic mutations, which were more prevalent among the high-risk than the low-risk group (P < 0.05). Alterations in PTEN, ERBB2 and BRCA1/2 were associated with disease relapse events in luminal B1 breast cancer.
Conclusions:
PIK3CA, FGFR1 and BRCA1/2 were the most prevalent actionable alterations among Taiwanese luminal B1 breast cancer. Moreover, PTEN and BRCA1/2 was significantly associated with disease relapse.
Wet activation and electrochemical performance of the residual carbon from coal gasification fine slag
Authors: Han Baobao , Zhu Jiaqi , Liu Rui , Xin Guoxiang , Wang Yao , Gao Jianmin , Song Jinling 
Published: August 2023

Abstract
It is difficult to utilize the large amount of gasification slag produced in the coal chemical industry, which is a major problem in solid waste resource utilization. The residual carbon from coal gasification fine slag (RC) was actived by KOH wet method. The activation temperature (110℃, 130℃, 150℃, 170℃) was tuned to regulate the pore structure of the RC and explore the effects on the electrochemical performance. When the active temperature was 150℃, the electrochemical performance of the as-actived RC-150 exhibited the best among the actived materials. The specific capacitance of RC-150 reached 109.0 F/g at a current density of 0.5 A/g. The energy density of the symmetric supercapacitor using RC-150 as active materials reached 3.78 Wh/Kg at a current density of 2 A/g. After 10 000 cycles, the capacitance was able to maintain 103.81% of the initial value, showing better cycle stability. The results could be attributed to the improvement of pore structure, the removal of oxide impurities and the modulation of graphitization degree during the activation process.
Tribological and Mechanical laboratory Analysis of DLC as substitutes for hard chromium coating in view of Cold Rolling of High Carbon Steel Strips
Authors: Ould Choumad , Gachon Yves , Badiche Xavier , Montmitonnet Pierre 
Published: August 2023

Abstract
Two types of wear-resistant, heavy-duty rolls are widely used in high carbon steel strips cold rolling: High Speed Steel (HSS) rolls and chromium-electroplated rolls. Lower costs and replacement of chromium, criticized because of its effects on health, are two reasons to look for another coating solution for rolling mill rolls. To be economically viable, it must maintain surface quality in accordance with customer requirements, and / or allow larger strip reduction per pass, and / or work at higher rolling speed and / or resist wear better so as to roll more coils between roll changes. Different phenomena, strongly related to the nature of roll surface, must be controlled in order to benefit from such improvements: severe adhesive wear of strip and adhesive transfer (“pick up”, “roll coating”), abrasive wear of rolls. DLC coatings are widely used in various tribological applications. Their resistance to wear and adhesion gives them a high potential in rolling. The interest of a hydrogenated DLC coating (here, with 20 at.% H) has therefore been studied for this application. The proposed paper analyzes its tribological and mechanical characteristics under conditions relevant to rolling processes, using two well adapted tests. Its resistance to transfer, severe adhesive wear, abrasive wear and fatigue is evaluated and compared with reference materials used in the rolling industry, uncoated and chrome-coated HSS, but also with other PVD coatings (TiN, CrN, TiBN) already described in previous papers. The results show the good potential of this coating in this application.
Phonetic Accommodation in Non-native Directed Speech Supports L2 Word Learning and Pronunciation
Authors: Piazza Giorgio , Kalashnikova Marina , Martin Clara D. 
Published: August 2023

Abstract
This study assessed whether Non-native Directed Speech (NNDS) facilitates second language (L2) learning, specifically L2 word learning and production. Spanish participants (N=50) learned novel English words, presented either in NNDS or Native-Directed Speech (NDS), in two tasks: Recognition and Production. Recognition involved matching novel objects to their labels produced in NNDS or NDS. Production required participants to pronounce these objects’ labels. The novel words contained English vowel contrasts, which approximated Spanish vowel categories more (/ɪ-i/) or less (/æ-ɛ/). Participants assigned to the NNDS group recognized novel words faster and learned and pronounced the /ɪ-i/ contrast words better compared to participants in the NDS group. Participants’ ability to discriminate the target vowel contrasts was also assessed before and after the tasks, with no improvement detected in the two groups. These findings support the didactic assumption of NNDS, indicating the relevance of the phonetic adaptations in this register for successful L2 acquisition.
WITHDRAWN: Action detection of Objects Devices Using Deep Learning in IoT Applications
Authors: Rustemli Sabir , Alani Ahmed Yaseen Bishree , Şahin Gökhan 
Published: August 2023

Abstract
Internet of Things (IoT) technology is the communication and communication of smart technological devices with each other. However, with the development of the Internet of Things, the number of smart applications and connected devices is increasing day by day. Deep Learning (DL) method has become necessary to process the large amount of raw data collected, to further develop intelligence and application capabilities. It is seen that the majority of researchers focus on action perception. Direct action detection on IoT devices using deep learning is not a common method. Standard Deep Learning techniques are difficult to use in IoT devices as Deep Learning applications require high CPU, RAM and storage. In the study, unlike the use of deep learning techniques in IoT devices, the action detection process was carried out directly on the edge device. For this, mini-size Deep Learning (DL-Lite) techniques have been applied on the real IoT device. Comparison of IoT devices and mini Deep Learning techniques was carried out according to parameters such as detection accuracy, delay and device temperature resulting from the application of these techniques in IoT devices.
First report of leaf spot on Atractylodes lancea caused by Alternaria gaisen in Japan
Authors: Kawashimo Midori , Sakurai Miki 
Published: August 2023

Abstract
Atractylodes lancea is a medicinal plant that has been used in traditional Chinese and Japanese herbal medicines. In 2020, A. lancea plants with leaf spot were identified in Ibaraki, Japan. We isolated fungi from the leaf spot symptom, and in inoculation tests, the isolate reproduced these symptoms. The isolate was identified as Alternaria gaisen based on conidial morphology and sequences of three genomic regions (rpb2, gapdh, and tef1). To our knowledge, this is the first report of leaf spot of A. lancea caused by A. gaisen.
In-vivo study of three-dimensional, cervical segmental motion characteristics following anterior cervical fusion with different numbers of fusion levels
Authors: Zhong Yanlong , Zhang Zizhen , Luo Shiwei , Chen Gongxin , Li Chao , Yang Jie , Li Jidong , Li Guoan , Wang Shaobai , Wan Zongmiao 
Published: August 2023

Abstract
Background
The pathogenesis of adjacent segment degeneration (ASD) and the biomechanical influence following anterior cervical fusion (ACF) surgery with different number of fusion levels remains controversial. The objective of this study was to investigate the effect of different numbers of fusion levels on adjacent segments and overall cervical kinematics.
Methods
From October 2018 to August 2020, patients who underwent ACF at our department were screened for enrollment in the study and divided into three groups based on the number of fusion levels: one-, two- and three-level. All subjects underwent pre- and post-operative cervical spine cone beam computed tomography(CBCT)scans at 7 functional positions. The range of motion (ROM) of the superior and inferior adjacent segments as well as the overall cervical, were measured using a 3D-3D registration technique. Changes in the pre- and post-operative ROMs were calculated for each patient. Clinical outcomes were assessed using the cervical spine stiffness scale, VAS and the JOA Scores.
Results
At the superior and inferior adjacent segment, the ROM of three-level were all greater than one-level during left-right bending and flexion-extension (p＜0.05). The global ROM of three-level were significantly smaller than one-level during flexion-extension (p＜0.05). The postoperative ROM of C4-C5 segment was significantly greater than that of C2-C3 (p＜0.05) at flexion-extension. The post-operative VAS、JOA scores showed no significant differences between the three groups(p > 0.05). But the post-operative cervical spine stiffness scores of the three-level was higher than that of the one-level (p＜0.05).
Conclusion
Three-level ACF had increased compensatory motion at the superior or inferior adjacent segments than one-level group at last follow-up and were probably to develop adjacent segment degeneration, which is more common in C4-C5 segment. Compared with the one-level fusion, the global ROM of the three-level decreased slightly and had little effect on the postoperative function of cervical spine.
Comprehensive Analysis of m6A Modification in Immune Infiltration, Metabolism and Drug Resistance in Hepatocellular Carcinoma
Authors: Shi Yunxing , Li Kai , Yuan Yichuan , Wang Chenwei , Yang Zhiwen , Zuo Dinglan , Niu Yi , Qiu Jiliang , Li Binkui , Yuan Yunfei , He Wei 
Published: August 2023

Abstract
N6-methyladenosine (m6A) is important in regulating mRNA stability, splicing, and translation, and it also contributes to tumor development. However, there is still limited understanding of the comprehensive effects of m6A modification patterns on the tumor immune microenvironment, metabolism, and drug resistance in hepatocellular carcinoma (HCC). In this study, we utilized unsupervised clustering based on the expression of 23 m6A regulators to identify m6A clusters. We identified differential m6A modification patterns and characterized m6A-gene-cluster A, which exhibited poorer survival rates, a higher abundance of Treg cells, and increased expression of TGFβ in the tumor microenvironment (TME). Additionally, m6A-gene-cluster A demonstrated higher levels of glycolysis activity, cholesterol metabolism, and fatty acid biosynthesis. We also found that the m6A score was associated with prognosis and drug resistance. Patients with a low m6A score experienced worse prognoses, which were linked to an abundance of Treg cells, upregulation of TGFβ, and increased metabolic activity. HCC patients with a higher m6A score showed improved prognosis following sorafenib treatment and immunotherapy. In conclusion, we reveals the association between m6A modification patterns and the tumor immune microenvironment, metabolism, and drug resistance in HCC. Furthermore, the m6A score holds potential as a predictive factor for the efficacy of targeted therapy and immunotherapy in HCC.