bluuebunny/crossref_metadata_2025_split huggingface dataset:
Targeting Nerve Fiber Outgrowth Inhibition After Experimental Spinal Cord Injury: A Systematic Review and Meta-analysis of Chondroitinase ABC
Authors: Khanteymoori Alireza , Peterson Clayton , Atamny Roza , Hohenhaus Marc , Beck Jürgen , Howells David W. , Schwab Jan M. , Watzlawick Ralf 
Published: January 2025

Background: Spinal cord injury (SCI) can impair motor, sensory, and autonomic function. The formation of the glial scar comprises protective as well as inhibitory neurite outgrowth properties operated by the deposition of chondroitin sulfate proteoglycans (CSPG). Chondroitinase ABC (ChABC) can degrade CSPG and foster neuroaxonal plasticity as a therapeutic approach to restore locomotor function after SCI. Objectives: To systematically review experimental ChABC treatments after SCI and assess their efficacy for locomotor function a comprehensive literature search was conducted following pre-registered Prospero Study protocol, selecting animal studies evaluating neurobehavioral outcomes after traumatic SCI followed by the calculation of normalized effect sizes applying meta-analysis and meta-regression methodology. Additional analyses were performed to investigate the impact of animal type, strain, sex, sample size, injury models, level of injury, and treatment duration. Results: Within the overall analysis of 1066 animals, a considerable amount of heterogeneity was observed. A subgroup analysis comprising experiments applying the same neurobehavioral measurement (blood–brain barrier/Basso-Mouse-Scale [BMS]-subgroup) demonstrated a 15.9% (95% CI = 11.3%-20.6%) improvement in locomotor outcomes. Different experimental characteristics influenced neurological recovery, including sex, level of injury, used anesthetic, reported dosage of ChABC treatment, the timepoint of assessment and perioperative temperature control. Sensitivity analysis applying Trim and Fill identified 19 hypothetical missing experiments suggestive of reporting bias. Conclusion: Reporting bias in experimental SCI research is prevalent and not limited to a specific intervention. ChABC treatment can exert beneficial effects on locomotor recovery after SCI.
Correlation of scapular Dyskinesia and hand grip strength in students with smartphone addiction pursuing a bachelor's degree in healthcare profession education
Authors: Shetty Mamta , Sathyadas Sreemaya , Sabu Thomas Aliza , Jayan Veenedathu Liya , Vichare Revati , Thakkar Prathna , Surve Adnyey 
Published: January 2025

Background Hand grip strength is a critical indicator of upper extremity function, especially important in healthcare professions. Effective upper extremity function relies on proximal stability from the scapula and distal mobility from hand actions. This study aimed to examine the relationship between scapular dyskinesia and hand grip strength in healthcare students with smartphone addiction. Methodology A descriptive cross-sectional study was conducted with 63 students with smartphone addiction. Scapular dyskinesia was measured using Lateral Scapular Slide Test (LSST) at neutral, 45 and 90 degrees. Hand grip strength was measured using the Jamar Hydraulic hand dynamometer. The Short version of Smartphone Addiction Scale (SAS-SV) questionnaire assessed the severity of smartphone addiction, while the Disability of Arm, Shoulder and Hand (DASH) questionnaire evaluated the impact on upper extremity function. Results A negative correlation was found between LSST at 45degrees and hand grip strength. However, no significant correlation was observed between SAS-SV, DASH, and scapular dyskinesia. Conclusion Findings of the present study report a negative association between scapular dyskinesia at 45 degrees of LSST and hand grip strength. This suggests greater the scapular dyskinesis, lower will be the hand grip strength.
Analysis and characterization of interhypothalamic adhesions in adults: No longer only a pediatric finding
Authors: Tuncer Onur , Harrell Alan D , Nascene David 
Published: January 2025

Background and purpose Inter-hypothalamic adhesions (IHAs) are parenchymal tissue bridges traversing the third ventricle, previously reported only in the pediatric population. We aim to understand the prevalence of IHA in the adult population, assess their size and location, and ultimately investigate whether IHA volumes correlate with age. Materials and methods Patients who underwent routine high-resolution 3D T2WI MRI studies of the temporal bone/internal auditory canal at an otolaryngology clinic between 2008 and 2014 were consecutively selected. The presence of IHAs was confirmed when a parenchymal structure could be traced across the third ventricle, connecting bilateral hypothalamus, and was visible in at least two planes. They were classified based on their location within the third ventricle, considering their connection with hypothalamic nuclei. Patient clinical information were collected from electronic charts. The prevalence and volumes of IHAs were calculated. Associations between age and IHA volume, as well as between IHA type, age, volume, and gender, were analyzed. Results 779 patients, with a mean age of 54.7 years were included. Among them, 44 IHAs were identified within 41 patients, resulting in a prevalence of 5.26% in our cohort. Type 1 IHA was the most frequently encountered type, comprising 70.5% of all cases. No significant correlation was observed between IHA volumes and age. Additionally, no associations were found between IHA type and age, volume, or gender. Conclusion IHAs are not exclusive to the pediatric population; they are also present in adults, with a prevalence of 5.26% in patients undergoing temporal bone/internal auditory canal MRI.
Ubiquitin specific peptidase 11 knockdown slows Huntington’s disease progression via regulating mitochondrial dysfunction and neuronal damage depending on PTEN-mediated AKT pathway
Authors: Gao Bai , Jing Yuchen , Li Xi , Cong Shuyan 
Published: January 2025

Abstract
Background
Mitochondrial dysfunction and neuronal damage are major sign of cytopathology in Huntington’s disease (HD), a neurodegenerative disease. Ubiquitin specific peptidase 11 (USP11) is a deubiquitinating enzyme involved in various physiological processes through regulating protein degradation. However, its specific role in HD is unclear.

Methods
To interfere with USP11 expression, adeno-associated viruses 2 containing USP11-specific shRNA were injected into the bilateral striatum of 12-week-old R6/1 and WT mice. In vitro, the inducible PC12 cell model of HD was used in which the expression of an N-terminal truncation of huntingtin, with either wild type (Q23) or expanded polyglutamine (Q74) can be induced by the doxycycline. USP11 was knocked down to study its role in HD. The protein expression patterns in Q74 cells were quantified by label-free proteomics to further explore the target protein of USP11. Detecting the association between USP11 and Phosphatase and Tensin Homolog (PTEN) through Co-IP.

Results
Herein, USP11 was found to be upregulated in the striatum of R6/1 mice (an HD model with gradual development of symptoms) in an age-dependent manner. The spontaneous HD was alleviated by silencing USP11, as evidenced by improved locomotor activity and spatial memory, attenuated striatal atrophy in R6/1 mice, reduced accumulation of mutant huntingtin protein, and restored mitochondrial function in vitro and in vivo. The results of label-free proteomics revealed a significant change in the protein expression profile. Through functional enrichment, we focused on PTEN, known as a negative regulator of the AKT pathway. We demonstrated that USP11 downregulation promoted ubiquitination modification of PTEN and activated the AKT pathway, and PTEN overexpression reversed the effects of USP11 knockdown.

Conclusions
Collectively, USP11 knockdown protects R6/1 mouse neurons from oxidative stress by alleviating mitochondrial dysfunction, thereby preventing the HD progression. This is achieved by inhibiting PTEN expression, which in turn activates the AKT pathway. This study suggests that USP11-PTEN-AKT signaling pathway may be a new attractive therapeutic target for HD.
Tracing the paths of modular evolution by quantifying rearrangement events of protein domains
Authors: Coban Abdulbaki , Bornberg-Bauer Erich , Kemena Carsten 
Published: January 2025

Abstract
Background
Protein evolution is central to molecular adaptation and largely characterized by modular rearrangements of domains, the evolutionary and structural building blocks of proteins. Genetic events underlying protein rearrangements are relatively rare compared to changes of amino-acids. Therefore, these events can be used to characterize and reconstruct major events of molecular adaptation by comparing large data sets of proteomes.

Results
Here we determine, at unprecedented completeness, the rates of fusion, fission, emergence and loss of domains in five eukaryotic clades (monocots, eudicots, fungi, insects, vertebrates). By characterizing rearrangements that were previously considered “ambiguous” or “complex” we raise the fraction of resolved rearrangement events from previously ca. 60% to around 92%. We exemplify our method by analyzing the evolutionary histories of protein rearrangements in (i) the extracellular matrix, (ii) innate immunity across Eukaryota, Metazoa, and Vertebrata, and (iii) Toll-Like-Receptors in the innate immune system of Eukaryota. In all three cases we can find hot-spots of rearrangement events in their phylogeny which (i) can be related with major events of adaptation and (ii) which follow the emergence of new domains which become integrated into existing arrangements.

Conclusion
Our results demonstrate that, akin to the change at the level of amino acids, domain rearrangements follow a clock-like dynamic which can be well quantified and supports the concept of evolutionary tinkering. While many novel domain emergence events are ancient, emerged domains are quickly incorporated into a great number of proteins. In parallel, the observed rates of emergence of new domains are becoming smaller over time.
Inverse association between obesity and suicidal death risk
Authors: Lee Joonyub , Lee Seung-Hwan , Kim Mee-Kyoung , Kwon Hyuk-Sang , Yun Jae-Seung , Yang Yeoree , Yoon Kun-Ho , Cho Jae-Hyoung , Pae Chi-Un , Han Kyungdo , Son Jang Won 
Published: January 2025

Abstract
Background
Suicide is a significant yet preventable public health issue. Body mass index (BMI) is a readily measurable indicator associated with various health outcomes. However, the relationship between BMI and suicidal death risk is complex and warrants further investigation, particularly within contemporary, non-Western contexts with consideration of potential confounders. The purpose of this study was to investigate the relationship between BMI and the risk of suicidal death.

Methods
This study was nationwide, retrospective, observational study based on Korean National Health Insurance Service database. We analyzed 4,045,081 participants who were aged > 19 years and underwent national health surveillance in 2009. The participants were categorized according to their BMI (underweight: < 18.5 kg/m², normal weight: 18.5–23 kg/m², overweight: 23–25 kg/m², class I obesity: 25–30 kg/m², and class II obesity: > 30 kg/m²). The primary outcome was the death events caused by suicide which was defined by International Classification of Disorders (ICD-10) codes (X60–X84) and death records documented by the Korea National Statistical Office. Multivariate Cox proportional hazard regression analysis was performed to estimate the risk of suicidal death with respect to BMI categories after adjusting for potential confounders (age, sex, income, diabetes, hypertension, dyslipidemia, smoking, drinking, exercise, self-abuse, waist circumference, schizophrenia, bipolar disorder, eating disorder, cancer, anxiety, and substance use disorder).

Results
Underweight individuals had an increased risk (hazard ratio [HR] 1.44, 95% confidence interval [CI] 1.31–1.57) while overweight (HR 0.79, 95% CI 0.76–0.83), class I (HR 0.76, 95% CI 0.71–0.80) and class II obesity (HR 0.71, 95% CI 0.63–0.81) were associated with decreased risks of suicidal deaths compared to those of the normal weight individuals (BMI 18.5–23). This trend was consistent regardless of the presence of major depressive disorder (MDD) or the type of living arrangements of the participants.

Conclusions
Suicidal death risk was inversely correlated with BMI categories, independent of MDD or living arrangements. Our data suggests the importance of physiological factors associated with body mass in understanding suicidal death risk. Furthermore, these data provide valuable insights to where the public health resources should be invested to reduce suicidal death rates.
Young people’s proposals for tackling everyday challenges in order to improve mental health: a qualitative comparison study based on different socioeconomic neighborhoods
Authors: Gard Helena , Ingvarsdotter Karin , Isma Gabriella Elisabeth , Enskär Karin , Mangrio Elisabeth 
Published: January 2025

Abstract
Background
Everyday challenges and stress negatively affect young people’s mental health. Socioeconomic status (SES) is associated with different stressors and different stress-coping mechanisms. Many interventions target youth mental health, but few consider socioeconomic differences in the planning, implementation, or evaluation. In a Swedish context socioeconomic status is related with migration experience. The aim of the study was to explore proposals for tackling everyday challenges among young people from different socioeconomic neighborhoods.

Methods
Eight focus groups, with participants between 13 and 15 years old from eight schools, were conducted in the south of Sweden. The participants discussed proposals for tackling everyday challenges. Using comparative thematic analysis, the focus group transcripts were divided into two groups, based on the socioeconomic status of the school’s neighborhood, and analyzed comparatively. Most of the participants in low SES neighborhoods had foreign background and most of the participants in high SES neighborhoods were Swedish born.

Results
The analysis resulted in four shared themes between the two SES groups: society is responsible, school is responsible, parents are responsible, and I am responsible. The differences and similarities between the two groups are presented in sub-themes. Many of the proposals were similar between the two SES groups, but with different underlying issues and examples.

Conclusion
Both groups proposed that adults must listen more to young people in order to improve the everyday challenges young people face. However, the low SES group in general expressed both more frustration and more agency, compared to the high SES group. This could be important to consider when planning school-based mental health promotion in different socioeconomic neighborhoods.
Early childhood caries risk prediction using machine learning approaches in Bangladesh
Authors: Hasan Fardous , Tantawi Maha El , Haque Farzana , Foláyan Moréniké Oluwátóyìn , Virtanen Jorma I. 
Published: January 2025

Abstract
Background
In the last years, artificial intelligence (AI) has contributed to improving healthcare including dentistry. The objective of this study was to develop a machine learning (ML) model for early childhood caries (ECC) prediction by identifying crucial health behaviours within mother-child pairs.

Methods
For the analysis, we utilized a representative sample of 724 mothers with children under six years in Bangladesh. The study utilized both clinical and survey data. ECC was assessed using ICDAS II criteria in the clinical examinations. Recursive Feature Elimination (RFE) and Random Forest (RF) was applied to identify the optimal subsets of features. Random forest classifier (RFC), extreme gradient boosting (XGBoost), support vector machine (SVM), adaptive boosting (AdaBoost), and multi-layer perceptron (MLP) models were used to identify the best fitted model as the predictor of ECC. SHAP and MDG-MDA plots were visualized for model interpretability and identify significant predictors.

Results
The RFC model identified 10 features as the most relevant for ECC prediction obtained by RFE feature selection method. The features were: plaque score, age of child, mother’s education, number of siblings, age of mother, consumption of sweet, tooth cleaning tools, child’s tooth brushing frequency, helping child brushing, and use of F-toothpaste. The final ML model achieved an AUC-ROC score (0.77), accuracy (0.72), sensitivity (0.80) and F1 score (0.73) in the test set. Of the prediction model, dental plaque was the strongest predictor of ECC (MDG: 0.08, MDA: 0.10).

Conclusions
Our final ML model, integrating 10 key features, has the potential to predict ECC effectively in children under five years. Additional research is needed for validation and optimization across various groups.
Primary care utilisation in different patients’ profiles with cardiovascular risk factors
Authors: Malo Sara , Maldonado Lina , Rabanaque María José , Bengoa-Urrengoechea Irantzu , Castel-Feced Sara , Sánchez-Calavera María Antonia , Aguilar-Palacio Isabel 
Published: January 2025

Abstract
Background
This study aimed to identify profiles of patients with cardiovascular disease (CVD) risk factors, based on their sociodemographic and clinical characteristics, and to analyse how their complexity is related to their frequency of visits in Primary Care.

Methods
Observational longitudinal study conducted in the Spanish CArdiovascular Risk factors for HEalth Services research (CARhES) cohort. Individuals older than 15 with hypertension, type 2 diabetes mellitus (DM) and/or dyslipidaemia in 2017 were selected and followed until 2021. Cluster analyses were performed to identify patients’ profiles according to age, sex and morbidity burden. Characteristics and annual visits in Primary Care in the different profiles were described. Panel data models were applied to study the variability of the frequency of visits to both physicians and nurses in Primary Care in the profiles across different time points.

Results
In this population-based cohort of 446,998 individuals, different profiles were identified among those with hypertension, type 2 DM and/or dyslipidaemia. Profiles comprising the elderly showed the highest morbidity burden. Among the profiles of individuals under 80, those that included women had a higher burden than profiles with men. This higher complexity was associated with higher frequency of Primary Care visits, regardless of the patient’s socioeconomic level and depopulation level of his/her Basic Health Area.

Conclusions
Women and the elderly comprised the profiles with the highest morbidity burden and Primary Care attendance. Tailoring care and resources according to the complexity profile is essential to ensure that patients receive the best possible care based on their needs.
Development of a pandemic-related core set of quality indicators for quality and patient safety in University Hospitals in Germany
Authors: Evers Janis , Altschuck Natalie , Mehl Claudia , Rüthrich Lilly , Harst Lorenz , Walther Felix , Steidle Oliver , Suckow Arnt , Hecker Ruth , Schmitt Jochen , Geraedts Max 
Published: January 2025

Abstract
Background
The COVID-19 pandemic entailed a global health crisis, significantly affecting medical service delivery in Germany as well as elsewhere. While intensive care capacities were overloaded by COVID cases, not only elective cases but also non-COVID cases requiring urgent treatment unexpectedly decreased, potentially leading to a deterioration in health outcomes. However, these developments were only uncovered retrospectively. Especially university hospitals, which were meant to take on a central coordinating role, did not have detailed information on expected healthcare utilization, available resources and capacities, and the quality of medical care.
The experience of compromised healthcare and a lack of monitoring during the COVID-19 pandemic made it clear that healthcare systems should be better prepared. Therefore, the aim of this work was to develop a set of indicators suited to detect undesirable developments concerning the provision of inpatient healthcare.

Material & methods
The study employed a literature review, online surveys, expert interviews, and a multistep evaluation process to develop a core set of quality indicators (QIs) suitable for assessing the resilience of university hospitals during pandemics. This initial set of indicators was refined through consultations with a) quality and risk management officials from German university hospitals via an online survey and b) a diverse panel of experts.

Results
The comprehensive evaluation identified two primary strands: organizational/management indicators (Strand A, 60 indicators) and disease-specific clinical quality and patient safety indicators (Strand B, 20 indicators for critical conditions like stroke, myocardial infarction, and cancer.) Three additional indicators were added after a final expert panel meeting, resulting in a final set of 83 indicators.

Discussion and conclusion
The developed QIs mark a significant advancement in the operational preparedness of university hospitals for pandemics. The study contributes to quality management in healthcare during pandemics by creating the basis for a structured approach to pandemic preparedness and response. This unique set of QIs within the German context presents an opportunity for establishing quality improvement, underscoring the importance of a robust, adaptable quality management framework as a basis for safeguarding against future health crises.
SarkoLife: quality of life in patients undergoing multimodal soft tissue sarcoma treatment
Authors: Hoffmann Sebastian , Hoffmann Tabea , Potkrajcic Vlatko , Deinzer Christoph K. W. , Benzler Katrin , Zender Lars , Daigeler Adrien , Thiel Johannes Tobias 
Published: January 2025

Abstract
Objective
To assess the tolerability of multimodal therapy in soft tissue sarcoma patients, particularly with regard to their quality of life and level of distress.

Materials and methods
A retrospective cohort study enrolled individuals receiving sarcoma therapy at the sarcoma center of the University of Tuebingen between 2017 and 2022. Participants completed an online survey that included the EORTC’s questionnaire (QLQ-C30), coupled with the distress thermometer and demographic inquiries. The primary emphasis was on comparing three distinct modalities: Radiation, Chemotherapy and Surgery. The data were analysed performing one-way ANOVA.

Results
A total of 237 patients were included in the study. There was a significant difference (p < 0.001) in quality of life according to the EORTC scores (high score = high quality of life) between the different treatments: chemotherapy (mean: 26.8 [standard deviation: 19.5]), radiotherapy (51.0 [21.5]), and surgery (46.9 [28.3]). Similarly, a statistically significant discrepancy (p < 0.001) was found in average distress levels (high score = high level of distress) corresponding to each treatment type: radiation (5.0 [2.7]), surgery (6.0 [2.9]), and chemotherapy (7.4 [2.4]). The rates of patients willing to undergo the same treatment varied across groups, with the highest percentage observed in the surgery group (94.2%), followed by radiation (87.4%), and chemotherapy (73.5%).

Conclusion
Patients receiving multimodal therapy for soft tissue often find chemotherapy particularly demanding. Impairment of both quality of life and physical well-being is more likely and tends to be more severe compared with radiation or surgery. These observations should be taken into consideration when consenting patients and offering treatment plans.