bluuebunny/crossref_metadata_2025_split huggingface dataset:
Intraoperative motor-evoked potential with tetanic stimulation changes pre- and post-hemispherotomy
Authors: Sasaki Ryota , Tamura Kentaro , Takatani Tsunenori , Park Young-Soo , Nakagawa Ichiro 
Published: September 2023

Abstract
Careful examination of motor evoked potential (MEP) findings is critical to the safety of intraoperative neuromonitoring during neurosurgery. We reviewed the intraoperative MEP findings in a pediatric patient who had undergone hemispherotomy for refractory epilepsy. The patient was a four-year-and-two-month-old boy with extensive right cerebral hemisphere, drug-resistant epilepsy, left upper and lower extremity paralysis, and cognitive impairment. We examined intraoperative MEP results both before and after hemispherotomy. Post-hemispherotomy, MEPs were successfully elicited through transcranial electrical stimulation (TES) but not via direct cortical stimulation on the right side. Furthermore, TES on the right side, following hemispherotomy, led to a reduction in the MEP amplification effect resulting from tetanic stimulation of the left unilateral median and tibial nerves. Conversely, we observed the effects of MEP amplification during TES on the left side after tetanic stimulation of these nerves. Postoperatively, the patient underwent magnetic resonance imaging and electroencephalogram examinations, confirming the anatomical and electrophysiological completeness of the dissection. Notably, the seizures disappeared, and no apparent complications were observed. Collectively, our findings suggest that TES can still activate deep structures and elicit MEPs, even in cases where the corticospinal connections to the posterior limb of the internal capsule are entirely severed. Thalamocortical interactions may affect the MEP amplification, observed during tetanic stimulation. Injury to the corticospinal tracts of the white matter may be obscured on conventional MEP findings; however, it may be identified by MEP changes in tetanic stimulation.
The combination of Angelica sinensis polysaccharide and cisplatin promotes ferroptosis in cisplatin-resistant ovarian cancer cells by regulating GPX4, thereby reversing their resistance to cisplatin
Authors: Guo Weikang , Wang Wanyue , Lei Fei , Zheng Ruxin , Zhao Xinyao , Gu Yuze , Yang Mengdi , Tong Yunshun , Wang Yaoxian 
Published: September 2023

Abstract
Background
Angelica sinensis (Oliv.) Diels has been used for centuries in Chinese traditional medicine to treat gynecological ailments. Numerous studies indicate that Angelica sinensis polysaccharide (ASP), an extract from Angelica sinensis, can inhibit various forms of cancer. Nevertheless, the therapeutic potential of ASP for treating ovarian cancer remains largely unexplored.
Methods
The study investigated cell proliferation and invasion, as well as the effects of a combination of ASP and DDP after tumor formation in nude mice. Tumor size was monitored, and HE staining was conducted on tissue samples. The identification of key gene GPX4 was performed via RNA-seq and bioinformatic analysis. GPX4 was overexpressed using lentivirus transfection, and its expression was evaluated via RT-qPCR and western blot. Additionally, the ferroptosis of cells was assessed through the measurement of Fe2+, malondialdehyde (MDA), superoxide dismutase (SOD), and glutathione (GSH).
Results
The results indicated a synergistic effect of ASP combined with DDP, leading to better inhibition of proliferation and invasion of SKOV3/DDP cells. Similarly, ASP combined with DDP demonstrated tumor proliferation inhibition in tumor-forming nude mice, while maintaining good safety. Bioinformatics analysis of 843 differentially expressed genes (DEGs) revealed that the key gene GPX4 played a significant role in the mechanism of action. Furthermore, the expression of GPX4 was inhibited by ASP combined with DDP, which resulted in SKOV3/DDP inhibition of proliferation and invasion. The study also demonstrated that ASP combined with DDP led to increased levels of Fe2+ and MDA, while decreasing levels of GSH and SOD, suggesting the promotion ferroptosis of SKOV3/DDP cells.
Conclusions
The combination of ASP and DDP has the ability to inhibit the proliferation and invasion of SKOV3/DDP cells. Additionally, inhibiting GPX4 expression in SKOV3/DDP cells promotes ferroptosis.
Highl diverse cuticular hydrocarbon profiles but no evidence for aggression towards non-kin in the ambrosia beetle Xyleborinus saxesenii
Authors: MELET A , LEIBOLD V. , SCHMITT T. , BIEDERMANN P.H. 
Published: September 2023

Abstract
Animal societies have nestmate-recognition to protect against social cheaters and parasites. In most social insect societies individuals recognize and exclude any non-nestmate. There, the roles of cuticular hydrocarbons as recognition cues are well documented. Some ambrosia beetles live in cooperatively breeding societies, within nests that are almost completely isolated. Their fungus-farming and inbreeding habits within their nest ensure a nearly complete isolation. However, little is known about whether nestmate recognition is present and adaptive in these ambrosia beetle societies.
Here we studied the ability for nestmate recognition in the cooperatively breeding ambrosia beetle Xyleborus saxesenii, combining behavioural observations and cuticular hydrocarbon analyses. Laboratory nests of X. saxesenii were exposed to foreign adult females, from the same population, another population and another species and survival as well as behaviours of receivers and the donor individual were observed. We expected that increasing genetic distance would cause increasing distance in chemical profiles and increasing levels of behavioural exclusion and possibly mortality. Chemical profiles were different between populations and appeared as variable as in other highly social insects. However, we did not find any evidence for behavioural exclusion of foreign individuals. These results suggest that cuticular hydrocarbon profiles might be used for communication, but that exclusion of non-nestmates is either absent in X. saxesenii or agonistic encounters are so rare or subtle that they could not be detected by our method. In the first case it may be adaptive for inbreeding ambrosia beetle species to accept non-nestmate to increase genetic diversity within nests.
Exsitu assessment of potential free (FC) and immobilized cell (IC) bacteria used as engine oil (UEO) degraders in the degradation of UEO in liquid yeast extract minimal salt medium (YEMSM)
Authors: Elhamrouni Izeddin Abdalla , Ishak Mohd Yusoff , Jaber Saja Mahdey , Kadhum Safaa A , Johari Wan , Halimoon Normala , Awadh Hassan 
Published: September 2023

Abstract
Used engine oil (UEO) contains potentially toxic substances that harmful humans and the ecosystem. Its dangerous pollution requires customized, efficient adsorptive bioremediation. This study investigates the local soil bacteria that can remove petrol-UEO (shell 15–40) by metabolic capability in UEO-YEMSM, previously isolated from UEO-polluted soil in KL, Malaysia. The biosorptive bioremediation capabilities of the free cell (FC) formula for UEO have been studied using pure Ochrobacterium intermedium LMG 3301 and mixed culture consortia of Ochrobacterium intermedium LMG 3301 plus Bacillus paramycoides MCCC1A04098 (BC), with an inoculation size of 3 × 109 CFU/mL in 0.675% (v/v) UEO-YEMSM. To study the UEO adsorption bioremediation of IC systems and compare them to the same FC systems, pure and mixed bacteria cells were immobilized using alginate-attapulgite-calcium carbonate (AAC). On day 24, the IC systems exhibited the removal of nC9 to nC17 with a 30% increase in TPH obtained with O.intermedium and the 18% by O. intermedium + B. paramycoides (BC) in UEO, as compared to the same FC systems. The AAC-IC of O. intermedium and the BC also removed nC9-nC29 to 93% and 98%, demonstrating the adsorptive-biosorptive bioremediation capacity of IC-systems with efficient mass transfers of experimental effectiveness factor (η) values of 1.04, and1.05, close to FC system η = 1. The enhanced degradation and bacterial growth in the AAC-IC systems indicated the high potential of AAC beads to be applied in the insitu bioremediation of UEO-polluted soils/water environments in KL, Malaysia.
An investigation of the breaking strength of timber from different tree species used for school furniture in Walukuba Division, Jinja City, Uganda
Authors: Napulu George , Balimunshe John , balinya lincoln , Nzala Nicholas Walter , Kabagambe Musa 
Published: September 2023

Abstract
The study investigated about the breaking strength of wood in Walukuba Division Jinja City. In this study we aimed to: to determine the breaking strength of different wood types varying length and extension while keeping force and area constant and to determine the breaking strength of different wood types by varying force and length while keeping area and extension constant. In this experiment we studied timber from Eucalyptus, Maesopsis eminii and Markhamia because they are the most common species in the study area. The cantilever method was used where a known mass was attached to the free end of a wood rod and the extension was noted. The experiment was repeated with various masses from 100 to 600grams. We varied the length of wood and kept noting the extension this time round varying the length of the wooden rod while maintaining a load of 100g and noting the extension on the meter rule that was clamped vertically at the extreme end using a retort stand. The results showed the young’s modulus of elasticity of 1267.27,1164.74, and 709.72Nm− 2 for Eucalyptus, Musizi and Makhamia timber respectively. With these results we deduce that timber form Eucalyptus could withstand higher load without breaking as compared to others we recommend that Eucalyptus should be used in generation of furniture for institutions moving forward.
Identification and Validation of Golgi Apparatus-Related Signature for Predicting Prognosis and Immunotherapy Response in Breast Cancer
Authors: Chen Xin , Tang Pengting , Kong Ying , Chen Deqin , Tang Kejun 
Published: September 2023

Abstract
Background: The Golgi apparatus plays a pivotal role in various aspects of cancer. This study aims to investigate the predictive value of Golgi apparatus-related genes (GARGs) in breast cancer prognosis and immunotherapy response evaluation.
Methods: Transcriptional and clinical data from the TCGA-BRCA cohort and GSE96058 cohort were utilized to construct and validate a prognostic model for breast cancer using Cox regression analysis. Differences in immune landscape, somatic mutations, gene expression, drug sensitivity, and immunotherapy response between different risk groups were assessed. A prognostic nomogram for breast cancer was further developed and evaluated. qPCR and single-cell sequencing analysis were performed to validate the expression of GARGs.
Results: A total of 394 GARGs significantly associated with breast cancer prognosis were identified, leading to the construction of a prognostic risk feature comprising 10 GARGs. This feature effectively stratified breast cancer patients into high-risk and low-risk groups, with the high-risk group exhibiting significantly worse prognosis. Meanwhile, significant differences in cliniopathological features, immune infiltration, drug sensitivity, and immunotherapy response were observed between the high- and low-risk groups. The constructed nomogram incorporating these factors showed superior performance in prognostic assessment for breast cancer patients. Ultimately, the utilization of qPCR and single-cell sequencing techniques substantiated the disparate expression patterns of these prognostic genes in breast cancer.
Conclusions: Our findings demonstrate that a prognostic risk feature derived from GARGs holds promising application potential for predicting prognosis and evaluating immunotherapy response in breast cancer patients.
Biodegradable Materials of Oat Hulls, Starch, and Polybutylene Succinate Produced by Thermoplastic Injection
Authors: Silva Samuel Camilo , Carvalho Fabíola Azanha , Yamashita Fabio 
Published: September 2023

Abstract

The utilization of biopolymers blended with agro-industrial residues or by-products holds promise due to the potential for cost reduction and improvement of material properties. In this study, our aim was to develop biodegradable polymeric blends using PBS, starch, and oat hulls via thermoplastic injection. Seven different formulations were analyzed [F0, F20, F40, F60, F80, F100, and PBS], and various analyses were performed. The mechanical properties revealed that pure PBS and F0 presented the highest tensile strength and elongation at break respectively. Pure PBS and F100 demonstrated the lowest density. SEM of materials containing oat hulls displayed cylindrical-shaped fibers, with significant agglomeration and alignment likely due to extrusion orientation. The results demonstrate the successful production of a novel material using thermoplastic starch, PBS, and oat hulls through thermoplastic injection. These materials exhibited good processability. This presents a promising avenue for the development of cheaper and biodegradable materials with good processability to be produced in large scale, as they are environment friendly.
Local Behavior of Certain n-Dimensional Diffeomorphisms
Authors: Siller Tiago E. 
Published: September 2023

Abstract
The main purpouse of this paper is to prove that a \(C^1\)-diffeomorphism of the form \(\varphi(x) = x + (x_n\varphi_1(x), \ldots, x_n\varphi_{n-1}(x), x_n^2\varphi_n(x))\) is locally topologically equivalent to its linear part under a generic condition.
MSC Classification: 37C15 , 37C05 , 58K40
RDSL: an Efficient Retrieval Algorithm for Group Editing CRDT
Authors: Ou Yuran , Zhou Jian 
Published: September 2023

Abstract
Conflict-Free Replicated Data Types (CRDT) is a popular approach for underpinning group editing in many applications, providing features such as peer-to-peer and offline editing. While conventional approaches such as Operation Transformation (OT) can be challenging to implement those features, CRDT still faces a critical issue - inefficient data retrieval due to storing data in a data structure with linear complexity. To address the issue, we propose a novel retrieval algorithm called Relative Distance Skip List (RDSL). This algorithm utilizes a probabilistic balancing identifier structure, which is based on relative distances between original CRDT nodes. The RDSL algorithm is both time- and space-efficient, providing a stable and efficient solution for data retrieval in CRDT.
Evolution of the urinary microbiota in spinal cord injury patients with decubitus ulcer: a snapshot study
Authors: Morsli Madjid , Salipante Florian , Gelis Anthony , Magnan Chloé , Guigon Ghislaine , Lavigne Jean-Philippe , Sotto Albert , Dunyach-Remy Catherine 
Published: September 2023

Abstract
Background Current microbiome investigations of patients with pressure ulcers (PU) are mainly based on wound swabs and/or biopsy sequencing, leaving the colonization scenario unclear. Urinary microbiota has been never studied.Methods As part of the prospective ESCAFLOR study, we studied urinary microbiota of spinal cord injury (SCI) patients with PU without any urinary tract infection at the inclusion, collected at two times (at admission (D0) and after 28 days (D28)) during the patient's care (Trial Registration Number; South Mediterranean III; N°2014.04.01bis), investigated by 16S rDNA metagenomics next generation sequencing. Subgroup analyses were carried out between patients with wounds showing improved evolution versus stagnated/worsened wounds at D28. Analysis was done using EPISEQ® 16S and R software.Results Among the 12 studied patients, the urinary microbiota of patients with improved wound evolution at D28 (n = 6) presented a significant decrease of microbial diversity. This modification was associated with the presence of Proteobacteria phylum and an increase of Escherichia-Shigella (p = 0.005), as well as the presence of probiotic anaerobic bacteria Lactobacillus and Bifidobacterium. In contrast, Proteus abundance was significantly increased in urine of patients with stagnated/worsened wound evolution (n = 6) (p = 0.003).Conclusions This study proposes urinary microbiota as a complementary factor indirectly associated with the wound evolution and patient cure. It opens new perspectives for further investigations based on multiple body microbiome comparison to describe the complete scenario of the transmission dynamics of wound-colonizing microorganisms.
Genetic Variability Studies Based on Morphological and Physicochemical Characteristics Among Different Longan (Dimocarpus longan Lour.) Genotypes Under North Bihar Conditions
Authors: Kumar Ajay , Gupta A. K. , Kanth Neeharika , Yadav Sudheer Kumar 
Published: September 2023

Abstract

The present experiment was carried out in a randomized complete block design (RCBD) with three replications to study morphological variability, genetic variability, heritability, and genetic advance for different traits of twenty genotypes. The results indicated that the genotypes were diversified for all the quantitative and qualitative traits except a few qualitative traits. The phenotypic coefficient of variation (PCV) showed higher than the genotypic coefficient of variation (GCV) which indicates the meager effect of environment on the phenotypic expression of traits. The high heritability coupled with high genetic advance as percent of mean were recorded for the traits viz., plant height, trunk girth, crown diameter, tree volume, rachis and petiole length, duration of panicle imitation, number of days from panicle initiation to flowering, length and width of inflorescence, number of fruits per bunch, fruit, aril and seed weight, aril thickness, titratable acidity and TSS acid ratio. Therefore, these traits can be used as selection indices for indirect selection for the crop improvement programmes. On the basis of mean performance found best performing genotypes LGC-6 for yield attributing and fruit quality traits.
Enhanced catalysis of mechano-chemically synthesized KMgF3 catalysts for the Knoevenagel condensation
Authors: Iida Hajime , Sugiyama Shouya , Horie Tatsuya 
Published: September 2023

Abstract

Catalysis of KMgF3 catalysts for Knoevenagel condensation were enhanced by synthesizing mechano-chemically. The BET surface area and number of strongly basic site of KMgF3 were increased by applying greater mechanical energy (higher rotation rate) in a mechano-chemical process. Their increases were caused by stronger mechano-chemical effects such as the micronization of the particles and the creation of lattice defect on the surface, resulted in resulted in the enhancement of catalytic activity of KMgF3 for Knoevenagel condensation. The XPS analysis revealed that the element composition on the surface of KMgF3 was similar with K2MgF4 and indicated the possibility which the true active component for this reaction was K2MgF4 rather than KMgF3. The kinetic measurements revealed that the Knoevenagel condensation catalyzed by KMgF3 was regard as a first order reaction and apparent activation energy was estimated at 55.8 kJmol-1. A soluble component capable of acting as a catalyst was not present in the solution and the KMgF3 acted as a true solid catalyst.
Nutrient stoichiometry mediates the patchy coexistence of two subalpine grassland types
Authors: Hernández Juan Luis Mora , Armas-Herrera Cecilia , Badía-Villas David , Gómez Daniel 
Published: September 2023

Abstract
Background and Aims
High mountain areas often show high soil heterogeneity that allows for the close coexistence of plant species and communities with contrasting resource requirements. This study investigates the nutritional factors driving the mosaic distribution of Nardus stricta L. grasslands and chalk grasslands dominated by forbs in the subalpine southern Pyrenees (Spain).
Methods
The concentrations of C, N, P, S, K, Ca and fiber fractions were analyzed in herbage in relation to soil nutrient availability, soil β–glucosidase, urease, phosphatase and arylsulfatase activity and plant species and functional type composition.
Results
The chalk grassland showed higher N:P ratios in herbage and higher enzyme demand for P relative to N in the soil, which indicate a greater limitation of P versus N compared to the Nardus grassland. This limitation was related to the higher soil and plant Ca levels in the chalk grassland, where the calcareous bedrock lies close to the soil surface. In the Nardus grasslands, the alleviation of P limitation translated into higher productivity and replacement of forbs with taller graminoids rich in structural carbohydrates, which was accompanied by greater β–D–glucosidase activity. The plant N:K and P:K ratios indicated potential K deficiency in both grasslands, which resulted from a decreased uptake of K due to competition from Ca, as indicated by the correlation between plant K and the soil K+:Ca2+ ratio.
Conclusions
Our results highlight the effect of the heterogeneity of soil nutrient constraints, as mediated by their stoichiometry and controlled by local topography, on the biodiversity of high mountain ecosystems.
Fabrication of 3D aligned porous nanocellulose-based aerogel for rapid enrichment of the histidine-containing polypeptides
Authors: Ou Jiahui , Zhou Shuaipeng , Huang Chuanqing Q. , Tang Xiaoyue , Li Lu , Tian Yunong , Ye Yong 
Published: September 2023

Abstract

The high-throughput immobilized metal affinity chromatography (IMAC) material is urgently required to improve the efficiency of separation and purification of tagged-peptides in the related biomedical applications. Herein, the aminothiourea-modified nanocellulose (S-TOCN)-polyvinyl alcohol (PVA) composite aerogel with aligned pores was fabricated by directional freezing casting technique and then used to immobilize metal ion for the selective separation and purification of histidine-containing peptides from protein hydrolysates. The obtained S-TOCN-PVA materials with an oriented porous structure under the optimal conditions exhibited excellent mechanical properties, good adsorption performance and reusability. The adsorption capacity of S-TOCN-PVA aerogel for metal ions were as follows: Cu2+>Ni2+>Zn2+>Co2+, in which the higher metal ion chelating amount of S-TOCN-PVA composite aerogel for Cu2+ and Ni2+ reached 87.21 mg/g at pH=7 and 83.66 mg/g at pH=6 in the 2 h incubation time, respectively. The enrichment effects of Cu2+/S-TOCN-PVA and Ni2+/S-TOCN-PVA aerogels with selectivity of His-containing peptides from apricot kernel protein hydrolysates were up to 3.99 folds and 4.81 folds for one cycle, respectively. Moreover, the Ni2+/S-TOCN-PVA aerogels retained 87.5% of their initial adsorption capacity (848 mg/g) after 5 cycles, and the concentration was raised 39.05 folds. Molecular docking simulation showed that the oxygen of carboxyl group (-C-O and -C=O) and hydrogen of imidazole group at His molecule was primarily bound to the hydrogen of amino groups and oxygen of carboxyl group (C-O-C) on the structure of M2+/S-TOCN-PVA aerogels. The fabricated metal ion immobilized 3D aligned porous nanocellulose-based aerogels can be used as IMAC materials for rapid separation and purification of histidine-containing polypeptides from complicated biological samples.