bluuebunny/crossref_metadata_2025_split huggingface dataset:
Opportunities and obstacles in microbial synthesis of metal nanoparticles
Authors: Carmona Manuel , Poblete‐Castro Ignacio , Rai Mahendra , Turner Raymond J. 
Published: March 2023

AbstractMetallic nanoparticles (MeNPs) are widely used in many areas such as biomedicine, packaging, cosmetics, colourants, agriculture, antimicrobial agents, cleaning products, as components of electronic devices and nutritional supplements. In addition, some MeNPs exhibit quantum properties, making them suitable materials in the photonics, electronic and energy industries. Through the lens of technology, microbes can be considered nanofactories capable of producing enzymes, metabolites and capping materials involved in the synthesis, assembly and stabilization of MeNPs. This bioprocess is considered more ecofriendly and less energy intensive than the current chemical synthesis routes. However, microbial synthesis of MeNPs as an alternative method to the chemical synthesis of nanomaterials still faces some challenges that need to be solved. Some of these challenges are described in this Editorial.
Three unrelated chemoreceptors provide Pectobacterium atrosepticum with a broad‐spectrum amino acid sensing capability
Authors: Velando Félix , Matilla Miguel A. , Zhulin Igor B. , Krell Tino 
Published: March 2023

AbstractAmino acids are important nutrients and also serve as signals for diverse signal transduction pathways. Bacteria use chemoreceptors to recognize amino acid attractants and to navigate their gradients. In Escherichia coli two likely paralogous chemoreceptors Tsr and Tar detect 9 amino acids, whereas in Pseudomonas aeruginosa the paralogous chemoreceptors PctA, PctB and PctC detect 18 amino acids. Here, we show that the phytobacterium Pectobacterium atrosepticum uses the three non‐homologous chemoreceptors PacA, PacB and PacC to detect 19 proteinogenic and several non‐proteinogenic amino acids. PacB recognizes 18 proteinogenic amino acids as well as 8 non‐proteinogenic amino acids. PacB has a ligand preference for the three branched chain amino acids L‐leucine, L‐valine and L‐isoleucine. PacA detects L‐proline next to several quaternary amines. The third chemoreceptor, PacC, is an ortholog of E. coli Tsr and the only one of the 36 P. atrosepticum chemoreceptors that is encoded in the cluster of chemosensory pathway genes. Surprisingly, in contrast to Tsr, which primarily senses serine, PacC recognizes aspartate as the major chemoeffector but not serine. Our results demonstrate that bacteria use various strategies to sense a wide range of amino acids and that it takes more than one chemoreceptor to achieve this goal.
A remote sensing tool for near real‐time monitoring of harmful algal blooms and turbidity in reservoirs
Authors: Pamula Abhiram S. P. , Gholizadeh Hamed , Krzmarzick Mark J. , Mausbach William E. , Lampert David J. 
Published: March 2023

AbstractHarmful algal blooms (HABs) diminish the utility of reservoirs for drinking water supply, irrigation, recreation, and ecosystem service provision. HABs decrease water quality and are a significant health concern in surface water bodies. Near real‐time monitoring of HABs in reservoirs and small water bodies is essential to understand the dynamics of turbidity and HAB formation. This study uses satellite imagery to remotely sense chlorophyll‐a concentrations (chl‐a), phycocyanin concentrations, and turbidity in two reservoirs, the Grand Lake O′ the Cherokees and Hudson Reservoir, OK, USA, to develop a tool for near real‐time monitoring of HABs. Landsat‐8 and Sentinel‐2 imagery from 2013 to 2017 and from 2015 to 2020 were used to train and test three different models that include multiple regression, support vector regression (SVR), and random forest regression (RFR). Performance was assessed by comparing the three models to estimate chl‐a, phycocyanin, and turbidity. The results showed that RFR achieved the best performance, with R2 values of 0.75, 0.82, and 0.79 for chl‐a, turbidity, and phycocyanin, while multiple regression had R2 values of 0.29, 0.51, and 0.46 and SVR had R2 values of 0.58, 0.62, and 0.61 on the testing datasets, respectively. This paper examines the potential of the developed open‐source satellite remote sensing tool for monitoring reservoirs in Oklahoma to assess spatial and temporal variations in surface water quality.
Targeting diacylglycerol kinase α impairs lung tumorigenesis by inhibiting cyclin D3
Authors: Zhou Dong , Liu Tao , Rao Xinrui , Jie Xiaohua , Chen Yunshang , Wu Zilong , Deng Huilin , Zhang Dan , Wang Jian , Wu Gang 
Published: March 2023

AbstractBackgroundDiacylglycerol kinase α (DGKA) is the first member discovered from the diacylglycerol kinase family, and it has been linked to the progression of various types of tumors. However, it is unclear whether DGKA is linked to the development of lung cancer.MethodsWe investigated the levels of DGKA in the lung cancer tissues. Cell growth assay, colony formation assay and EdU assay were used to examine the effects of DGKA‐targeted siRNAs/shRNAs/drugs on the proliferation of lung cancer cells in vitro. Xenograft mouse model was used to investigate the role of DGKA inhibitor ritanserin on the proliferation of lung cancer cells in vivo. The downstream target of DGKA in lung tumorigenesis was identified by RNA sequencing.ResultsDGKA is upregulated in the lung cancer cells. Functional assays and xenograft mouse model indicated that the proliferation ability of lung cancer cells was impaired after inhibiting DGKA. And cyclin D3(CCND3) is the downstream target of DGKA promoting lung cancer.ConclusionsOur study demonstrated that DGKA promotes lung tumorigenesis by regulating the CCND3 expression and hence it can be considered as a potential molecular biomarker to evaluate the prognosis of lung cancer patients. What's more, we also demonstrated the efficacy of ritanserin as a promising new medication for treating lung cancer.
The vanishing searches and audit fees: Evidence from Google's withdrawal from China
Authors: Zu Chunyu 
Published: March 2023

AbstractI investigate the impact of the disruption of free information access via search engines on audit fees using a quasi‐natural experiment provided by Google's withdrawal from China. Employing a difference‐in‐differences design, I document an increase in audit fees for firms with overseas business relative to firms without overseas business after Google's withdrawal. The results are robust to matched samples, placebo tests, alternative specifications, excluding alternative explanations and different event windows. This trend in audit fees suggests that Google's withdrawal hampers firms' foreign information streams and increases audit risk and audit effort. Consistent with this argument, after Google's withdrawal, firms with overseas business conduct more earnings management, pay more abnormal audit fees and experience longer audit report lags. Furthermore, the increase in audit fees is greater for firms with poor information environments, more retail investors or non‐Big 4 auditors. My findings suggest a potential auditing cost of restricting the free flow of public foreign information about firms.
Testing the Benefits of Public Deliberation
Authors: Minozzi William , Kennedy Ryan , Esterling Kevin M. , Neblo Michael A. , Jewell Ryan 
Published: March 2023

AbstractPublic deliberation grows increasingly prevalent yet remains costly in terms of money and time. Accordingly, some suggest supplanting talk‐based practices with individual, “deliberation within.” Yet we have little evidence either way on the additional benefits of public deliberation over its individual variant. We evaluate the benefits of public deliberation with a field experiment. With the cooperation of two sitting US Senators, we recruited several hundred of their constituents to deliberate on immigration reform. Participants were randomly assigned to either deliberate publicly in an online discussion, to deliberate individually, or to an information‐only control. Across several measures, public deliberation yielded more benefits than individual deliberation. We find, moreover, little evidence to ground worries that differences in education, race, conflict avoidance, gender, or gender composition of deliberating groups will render public talk less valuable than individual deliberation.
Outpatient vs inpatient induction of labor with oral misoprostol: A retrospective study
Authors: Hallén Natalie , Amini Mahdi , Wide‐Swensson Dag , Herbst Andreas 
Published: March 2023

AbstractIntroductionInduction of labor is one of the most common obstetrical procedures today, with a successively rising rate. With a limited number of hospital beds, the option of starting induction at home has gained increasing attention. The primary aim of this study was to compare the proportion of women achieving vaginal delivery and the duration of hospital stay before delivery in induction of labor with oral misoprostol starting at home and induction with oral misoprostol at the hospital, in a low‐risk population.Material and methodsWomen with home induction (n = 282) were individually matched to controls induced at the hospital during the same time period regarding parity, age, body mass index, labor unit and indication for induction.ResultsThe rates of vaginal birth were similar in outpatients and inpatients (84.8% vs 86.2%; p = 0.5). Time from hospital admission to delivery in the outpatient group was significantly shorter than in the inpatient group (12.8 vs 20.6 h; p < 0.001), as was total hospital stay (2 vs 3 days; p < 0.001). There were no significant differences between the groups in neonatal or maternal outcomes. One patient undergoing outpatient induction had an unplanned home birth.ConclusionsStarting induction at home reduced the time spent in hospital without affecting the vaginal delivery rate. Although underpowered to assess safety, this study did not show any differences in adverse maternal and perinatal outcomes between inpatients and outpatients. Further research is needed to evaluate the safety of outpatient induction of labor with misoprostol.
Accuracy and precision of sonographic fetal weight estimation in Sweden
Authors: Lindström Linda , Cnattingius Sven , Axelsson Ove , Granfors Michaela 
Published: March 2023

AbstractIntroductionFetal growth assessment by ultrasound is an essential part of modern obstetric care. The formula by Persson and Weldner for estimated fetal weight (EFW), used in Sweden since decades, has not yet been evaluated. The objective of this study was to evaluate accuracy and precision of the formula by Persson and Weldner, and to compare it to two other formulae using biparietal diameter instead of head circumference.Material and methodsThe study population consisted of 31 521 singleton pregnancies delivered at 22+0 gestational weeks or later, with an ultrasound EFW performed within 2 days before delivery, registered in the Swedish Pregnancy Register between 2014 and 2021. Fetal biometric ultrasound measurements were used to calculate EFW according to the formulae by Persson and Weldner, Hadlock 2 and Shepard. Bland–Altman analysis, systematic error (mean percentage error), random error (standard deviation [SD] of mean percentage error), proportion of weight estimates within ±10% of birthweight, and proportion with underestimated and overestimated weight was calculated. Moreover, calculations were made after stratification into small, appropriate, and large for gestational age (SGA, AGA and LGA), respectively, and gestational age at examination.ResultsFor the formula by Persson and Weldner, MPE was −2.7 (SD 8.9) and the proportion of EFW within ±10% from actual birthweight was 76.0%. MPE was largest for fetuses estimated as severe SGA (<3rd percentile, −5.4) and for the most preterm fetuses (<24 weeks, −5.4). For Hadlock 2 and Shepard's formulae, MPE were 3.9 (SD 8.9) and 3.4 (SD 9.7), respectively, and the proportions of EFW within ±10% from actual birthweight were 69.4% and 67.1%, respectively. MPE was largest for fetuses estimated as severe LGA (>97th percentile), 7.6 and 9.4, respectively.ConclusionsThe recommended Swedish formula by Persson and Weldner is generally accurate for fetal weight estimation. The systematic underestimation of EFW and random error is largest in extreme preterm and estimated SGA‐fetuses, which is of importance in clinical decision making. The accuracy of EFW with the formula by Persson and Weldner is as good as or better than Hadlock 2 and Shepard's formulae.
Key outcomes in childbirth: Development of a perinatal core outcome set for management of labor and delivery at or near term
Authors: Savchenko Julia , Asp Malin , Blomberg Marie , Elvander Charlotte , Hagman Anna , Pegelow Halvorsen Cecilia , Lindqvist Pelle , Nelander Maria , Skiöld Béatrice , Brismar Wendel Sophia 
Published: March 2023

AbstractIntroductionConsistency and relevance of perinatal outcome measures are necessary basics for obstetric research, audit, and clinical counseling. Still, there is an unwarranted variation in reported perinatal outcomes, which impairs research synthesis, validity, and implementation, as well as clinical benchmarking and longitudinal comparisons. The aim of this study was to develop a short‐term perinatal (fetal and neonatal) Core Outcome Set to be used in research and quality assurance of management of labor and delivery at or near term.Material and methodsThe methods were guided by the Core Outcome Measures in Effectiveness Trials Initiative Handbook. The project was prospectively registered on July 2, 2020 in the Core Outcome Measures in Effectiveness Trials (COMET) data base (reference number 1593). A list of potential outcomes was created based on a systematic review of studies evaluating interventions for peripartum management at or near term (≥34 weeks of gestation), including decisions regarding timing and type of onset of labor, intrapartum care, and mode of delivery. The list was entered into a two‐round Delphi survey with predefined consensus criteria. Participants (n = 67) included clinicians, researchers, lay persons with experience of childbirth (patient representatives), and other stakeholders. A consensus meeting was held to reach a final agreement.ResultsResponse rates were 82.1% (55/67) and 92.7% (51/55) for the first and second Delphi rounds, respectively. In total, 17 outcomes were included in the final core outcome set, reflecting mortality, health or morbidity, including asphyxia, central nervous system status, infection, neonatal resuscitation and admission, breastfeeding and mother‐infant interaction, operative delivery due to fetal distress, as well as birthweight and gestational age. Two of these outcomes were suggested by patient representatives.ConclusionsThe Swedish Perinatal Core Outcome Set (SPeCOS) study involved a broad circle of relevant stakeholders and reached consensus on a minimal set of perinatal outcomes that should be collected and reported in a standardized way in all future studies on management of labor and delivery at or near term, regardless of the specific population or condition studied. This could improve obstetric research, evidence synthesis, uptake, implementation, and adherence, as well as clinical practice, audit, and comparisons in childbirth care.
Evaluation of diagnostic and prognostic candidate biomarkers in drug‐induced liver injury vs. other forms of acute liver damage
Authors: Cueto‐Sánchez Alejandro , Niu Hao , Álvarez‐Álvarez Ismael , López‐Longarela Bárbara , Del Campo‐Herrera Enrique , Ortega‐Alonso Aida , García‐Cortés Miren , Pinazo‐Bandera José , Sanabria‐Cabrera Judith , Díaz‐Mochón Juan José , Lucena M. Isabel , Andrade Raúl J. , Stephens Camilla , Robles‐Díaz Mercedes 
Published: March 2023

AimsDetection and characterization of idiosyncratic drug‐induced liver injury (DILI) currently rely on standard liver tests, which are suboptimal in terms of specificity, sensitivity and prognosis. Therefore, DILI diagnosis can be delayed, with important consequences for the patient. In this study, we aimed to evaluate the potential of osteopontin, cytokeratin‐18 (caspase‐cleaved: ccK18 and total: K18), α‐glutathione‐S‐transferase and microRNA‐122 as new DILI biomarkers.MethodsSerial blood samples were collected from 32 DILI and 34 non‐DILI acute liver injury (ALI) cases and a single sample from 43 population controls without liver injury (HLC) and analysed using enzyme‐linked immunosorbent assay (ELISA) or single‐molecule arrays.ResultsAll biomarkers differentiated DILI and ALI from HLC with an area under receiver operator characteristic curve (AUC) value of >0.75 but were less efficient in distinguishing DILI from ALI, with ccK18 (0.79) and K18 (0.76) demonstrating highest potential. However, the AUC improved considerably (0.98) for ccK18 when comparing DILI and a subgroup of autoimmune hepatitis cases. Cytokeratin‐18, microRNA‐122 and α‐glutathione‐S‐transferase correlated well with traditional transaminases, while osteopontin correlated most strongly with the international normalized ratio (INR).ConclusionsccK18 appears promising in distinguishing DILI from autoimmune hepatitis but less so from other forms of acute liver injury. Osteopontin demonstrates prognostic potential with higher levels detected in more severe cases regardless of aetiology.
Prototypicality at the intersection of gender and sexual orientation
Authors: Klysing Amanda 
Published: March 2023

AbstractThe intersectional invisibility hypothesis (IIH) states that members of multiply marginalized groups experience intersectional invisibility by not being seen as prototypical for either of their constitutive groups due to the influence of heterocentrism and androcentrism. That is, a lesbian woman may not be represented in relation to either the category ‘woman’ or the category ‘homosexual people’. Two online experiments conducted in Sweden and the United Kingdom (N = 1923) tested predictions from the IIH at different intersections of specific genders (woman and man) and sexual orientations (heterosexuality, homosexuality and bisexuality) using an attribute generation task and direct similarity ratings. Results show strong support for the influence of heterocentrism: Only prototypes for heterosexual women/men match general prototypes for women/men. Androcentrism influenced the prototype for ‘homosexual people’, which had a stronger match with the prototype for gay men compared with lesbian women. In contrast, bisexual women were seen as more prototypical ‘bisexual people’ than bisexual men were. Psychological research conducted on general gender groups may therefore only be applicable to heterosexual individuals, while research on homosexual people in general may be applicable mainly to gay men.
The roles of RGS proteins in cardiometabolic disease
Authors: McNeill Samantha M. , Zhao Peishen 
Published: March 2023

G protein‐coupled receptors (GPCRs) are the most prominent receptors on the surface of the cell and play a central role in the regulation of cardiac and metabolic functions. GPCRs transmit extracellular stimuli to the interior of the cells by activating one or more heterotrimeric G proteins. The duration and intensity of G protein‐mediated signalling are tightly controlled by a large array of intracellular mediators, including the regulator of G protein signalling (RGS) proteins. RGS proteins selectively promote the GTPase activity of a subset of Gα subunits, thus serving as negative regulators in a pathway‐dependent manner. In the current review, we summarise the involvement of RGS proteins in cardiometabolic function with a focus on their tissue distribution, mechanisms of action and dysregulation under various disease conditions. We also discuss the potential therapeutic applications for targeting RGS proteins in treating cardiometabolic conditions and current progress in developing RGS modulators.LINKED ARTICLESThis article is part of a themed issue Therapeutic Targeting of G Protein‐Coupled Receptors: hot topics from the Australasian Society of Clinical and Experimental Pharmacologists and Toxicologists 2021 Virtual Annual Scientific Meeting. To view the other articles in this section visit http://onlinelibrary.wiley.com/doi/10.1111/bph.v181.14/issuetoc
The application of targeted protein degradation technologies to G protein‐coupled receptors
Authors: Keen Alastair C. , Jörg Manuela , Halls Michelle L. 
Published: March 2023

The ubiquitin‐proteasome system is one of the major pathways for the degradation of cellular proteins. In recent years, methods have been developed to exploit the ubiquitin‐proteasome system to artificially degrade target proteins. Targeted protein degraders are extremely useful as biological tools for discovery research. They have also been developed as novel therapeutics with several targeted protein degraders currently in clinical trials. However, almost all targeted protein degrader technologies have been developed for cytosolic proteins. The G protein‐coupled receptor (GPCR) superfamily is one of the most important classes of drug targets, yet only limited examples of GPCR degradation exist. Here, we review these examples and provide a perspective on the different strategies that have been used to apply targeted protein degradation to GPCRs. We also discuss whether alternative approaches that have been used to degrade other integral membrane proteins could be applied to the degradation of GPCRs.LINKED ARTICLESThis article is part of a themed issue Therapeutic Targeting of G Protein‐Coupled Receptors: hot topics from the Australasian Society of Clinical and Experimental Pharmacologists and Toxicologists 2021 Virtual Annual Scientific Meeting. To view the other articles in this section visit http://onlinelibrary.wiley.com/doi/10.1111/bph.v181.14/issuetoc