bluuebunny/crossref_metadata_2025_split huggingface dataset:
IMPLEMENTATION OF DRUG SUPPLY CHAIN MANAGEMENT IN THE PRACTICE OF THT-KL SPECIALISTS
Authors: Putra Ragil Andika , Syafwan Havid , Nasution Akmal 
Published: April 2023

Abstract: THT-KL Specialist Practice Dr. Julius Dariar is a practice that provides medical services and sells medicines for ear, nose, throat and head and neck surgery. The problem experienced is that drug preparation is still done manually, so it requires time, effort and a high level of accuracy and precision. This study aims to design an information system that can process incoming and outgoing drug supply data at the THT Specialist Practice-KL Dr. Julius Dariar by implementing Supply Chain Management. The result of his research is the design of a website-based Supply Chain Management application that can be used by users in managing drug stocks in the practice of THT-KL specialist Dr. Julius Dariar. Keywords: Practice; SCM; Specialist; THT-KL Abstrak: Praktek Spesialis THT-KL Dr. Julius Dariar merupakan salah satu praktek yang melayani jasa pengobatan dan penjualan obat mengenai telinga, hidung, tenggorokan, dan bedah kepala leher. Adapun masalah yang dialami ialah penyiapan obat masih dilakukan dengan manual, sehingga memerlukan waktu, tenaga serta tingkat ketelitian dan kecermatan yang tinggi. Penelitian ini bertujuan untuk merancang sistem informasi yang dapat mengolah data persediaan obat masuk dan keluar di Praktek Spesialis THT-KL Dr. Julius Dariar dengan menerapkan Supply Chain Management. Hasil penelitiannya adalah rancangan aplikasi Supply Chain Management berbasis website yang dapat digunakan oleh user dalam pengelolaan stok obat-obatan pada praktik spesialis THT-KL dr.Julius Dariar. Kata kunci: Praktik; SCM; Spesialis; THT-KL
A META-SYNTHESIS OF VIRTUAL REALITY IN LEARNING IN INDONESIA
Authors: Sapta Andy , Gafur Abd , Jameel Ami Hibatul 
Published: April 2023

Abstract: This research was conducted in the form of a literature study. This study investigates the use of Virtual Reality (VR) in teaching, including VR methods and their application to learning. In the literature study, the database used comes from Garuda (Garuda Digital Reference), published by The Ministry of Education, Culture, Research, and Technology. The results of the literature study obtained data that 157 articles discuss the use of VR in learning from 2012 - 2022 and in the majority of VR development using the 3D Modeling method, as much as 43%. Overall, using VR in learning provides positive results that can improve learning outcomes. Three essential things must be considered in the development of VR, namely: (1) the cost of operating VR technology, (2) Accessibility that makes it easy for teachers and respondents to access the technology, (3) Setting up a virtual environment to create a practical learning experience for respondents. Keywords: development; learning; virtual reality. Abstrak: Penelitian ini dilakukan dalam bentuk studi literatur. Dalam penelitian ini, menyelidikan penggunaan VR dalam pembejaran, meliputi metode VR yang digunakan dan penerapannya pada pembelajaran. Dalam studi literature, basis data yang digunakan berasal dari Garuda (Garuda Rujukan Digital) yang dipublikasi oleh The Ministry of Education, Culture, Reseach, and Technology. Dari hasil studi literature diperoleh data bahwa terdapat 157 artikel yang membahas tentang penggunaan VR dalam pembelajaran dari tahun 2012 – 2022. Secara mayoritas pengembangan VR dengan menggunakan metode 3D Modelling, sebanyak 43%. Secara keseluruhan penggunaan VR dalam pembelajaran memberikan hasil yang positif dapat meningkatkan hasil belajar. Terdapat tiga hal penting yang harus diperhatikan dalam pengembangan VR, yaitu: (1) biaya pengoperasian teknologi VR, (2) Aksesibilitas yang memudahkan pengajar dan responden untuk mengakses teknologi tersebut (3) Menyiapkan lingkungan maya (virtual environment) agar menciptakan pengalaman belajar yang efektif bagi responden. Kata kunci: pembelajaran; pengembangan; virtual reality
Hubungan Pengunaan Gadget Terhadap Interaksi Sosial dan Kecemasan (Nomophobia) pada Remaja Di RW 02 Dusun Keling
Authors: Juwariyah Siti , Sari Ni Made Ayu Wulan , Amalia Putri 
Published: April 2023

Interaksi sosial merupakan hubungan yang erat kaitannya dengan hubungan antar individu. Interaksi sosial individu ini terjadi saat individu satu dengan individu yang lain saling bertemu, berjabat tangan, saling berbicara dan saling mempengaruhi dalam pikiran dan tindakan. Seseorang menjadi sulit bertahan hidup jika tidak menjalin interaksi sosial. Dampak negatif penggunaan gadget yang ditimbulkan ini dapat menyebabkan remaja menjadi sering cemas dan tegang, bahkan dampaknya juga dapat menyerang mental para remaja dan mereka cenderung menjadi pribadi yang penakut dan tidak percaya diri, karena kecanduan gadget ini, para remaja menjadi tidak memiliki banyak teman berbicara. Tujuan penelitian ini untuk menganalisis hubungan penggunaan gadget terhadap interaksi sosial dan kecemasan (normophobia). Metode penelitian menggunakan desain penelitian deskriptif analitik dengan pendekatan cross sectional. Hasil penelitian di dapatkan hasil analisis statistik dengan uji Chi Square untuk menilai hubungan penggunaan gadget terhadap interaksi sosial pada remaja, didapatkan nilai ⍴-value 0,006 (⍴<0,05) sehingga dapat disimpulkan bahwa ada hubungan antara penggunaan gadget terhadap interaksi sosial pada remaja. Uji Chi square untuk menilai hubungan penggunaan gadget dengan kecemasan nomophobia di dapatkan nilai ⍴-value 0,023 (⍴<0,05) yang berarti terdapat hubungan antara penggunaan gadget terhadap nomophobia pada remaja. Semakin sering gadget mendampingi penggunanya dalam kehidupan sehari-hari maka semakin tinggi tingkat kecemasan yang dialami ketika remaja tidak memiliki akses terhadap gadget nya. Faktor yang mempengaruhi kecemasan (nomophobia) selain penggunaan gawai adalah kontrol diri. Kontrol diri yang baik akan menjauhkan seseorang dari kecanduan pada gadget, dan tentunya individu akan terhindar dari masalah kecemasan bila berada jauh dari gadget atau kecemasan (nomophobia). Kontrol diri yang baik akan membuat individu mengatur penggunaan gadget nya agar sesuai dengan kebutuhan dan tidak berlebihan.
Improving field legume nodulation by crushing nodules onto seeds: implications for small-scale farmers
Authors: Pudasaini Roshan , Hewedy Omar A. , Raizada Manish N. 
Published: April 2023

One billion people globally suffer from protein (amino acid) malnutrition. Grain legumes represent a solution. They recruit symbiotic rhizobia bacteria from soil into root nodules, where the rhizobia convert atmospheric nitrogen gas (N2) into ammonia (NH3) which serves as a building block for chlorophyll and protein. However, when a legume species is newly introduced to a region, yields can be low due to incompatible soil rhizobia. Millions of subsistence legume farmers can benefit from inoculation with exotic rhizobia bacteria, but many subsistence farmers especially in Africa do not benefit from commercial inoculants due to real-world constraints. Here, in a sequential series of indoor and outdoor experiments, we show that root nodules (rhizobia habitats) can be harvested and crushed onto legume seeds, ultimately improving nodulation and chlorophyll under field conditions. 16S rRNA metagenomic sequencing confirmed that nodule crushing onto seeds effectively transferred rhizobia to next-generation nodules. Therefore, nodule crushing represents a simple method to diffuse elite rhizobia strains. However, exotic rhizobia come with risks and limitations. Therefore, in addition to diffusing elite rhizobia, we propose that this simple, decentralized technology can also empower smallholders to improve indigenous strains or indigenize exotic strains by repeated nodule crushing from healthy plants.
Statistical analysis of ionospheric vertical total electron content anomalies before global Mw≥6.0 shallow earthquakes during 2000–2020
Authors: Ma Yifang , Zhou Xiaohui , Yang Yilin , Hu Leyin , Dong Hongyan , Yan Rui 
Published: April 2023

To quantitatively investigate the relationship between earthquakes and ionospheric anomalies, this paper presents a statistical study of pre-earthquake vertical total electron content (VTEC) variations. A total of 1522 shallow (≤60 km) strong (Mw≥6.0) earthquakes in the global area during 2000-2020 are selected, and classified according to different magnitudes, latitudes and focal depths. A quartile-based process with different lengths of sliding windows, equaling 10 days, 15 days and 27 days, respectively, has been utilized to detect VTEC anomalies. The abnormal level is first defined, and then VTEC anomalies occurrence probabilities (Po) and occurrence rates (PE) within 1-10 days before 1522 earthquakes have been calculated. Besides, VTEC anomalies occurrence rates of the background days (PN) are also calculated. The results show that the significant correlation between Po and epicentral latitudinal locations could be observed within 1-10 days before earthquakes. The values of Po increase with larger magnitudes in the equatorial and low-latitude regions, but decrease with greater magnitudes in the mid- and high-latitude regions to some degree. Within 1-5 days before earthquakes, the overall trend of PE shows an increase with larger magnitudes, but the correlation between the values of PE and magnitudes is relatively weak in the southern mid- and high-latitude regions. There is no evident causality between PN and the magnitude, and most of the values of PE/PN are larger than 1, indicating that VTEC anomalies within a few days before earthquakes are probably related with the forthcoming earthquakes. Moreover, when the abnormal level exceeds 60%, different sliding window lengths have a significant impact on the values of Po and PE in the mid- and high-latitude regions. In particular, there are obvious systematic deviations between the values of Po obtained from different sliding windows in the southern mid- and high-latitude regions. However, the selection of the optimal sliding window needs to be further studied.
Experienced, trauma-informed teachers working in remote Australia: What is required for their work to be effective?
Authors: Brown Meegan , L’Estrange Lyra 
Published: April 2023

IntroductionIn remote education settings in Australia, experienced teachers who can effectively support students impacted by trauma are essential. Remote communities are unique yet are in many ways vulnerable to trauma as they face higher rates of disadvantage and exposure to traumatic events, including natural disasters and domestic and family violence. This is compounded by a lack of access to effective supports due to the tyranny of distance. Also, First Nations peoples living in remote areas continue to endure the ongoing and traumatic impacts of a violent and disruptive colonization.MethodsThe qualitative research study detailed in this article explored the requirements for the work of experienced, trauma-informed teachers in remote Australia to be effective, adding an important and unique perspective to the research evidence that is not often considered. Seven teachers from remote Australia completed a short, online questionnaire and participated in a focus group interview which was analyzed thematically.ResultsThemes emerging from the focus group data indicated that specific and contextualized preparation and support for teachers is required for them to do their work effectively. For remote Australian settings this means preparing teachers with cultural awareness and relevant trauma-informed training. Further, the wellbeing of these remote educators is often compromised, and addressing systemic factors such as adequate preparation of their colleagues and support to access relevant ongoing professional learning is needed.DiscussionRemote teaching work in Australia is complex, and while the current study is small and exploratory in nature, the findings highlight some of the real-world impacts of these issues at a community and individual teacher level that have not been previously explored.
Bone marrow mesenchymal stem cells derived exosomal miRNAs can modulate diabetic bone-fat imbalance
Authors: Han Fei , Wang Chao , Cheng Peng , Liu Ting , Wang Wei-Shan 
Published: April 2023

BackgroundDiabetes mellitus is a chronic metabolic disease with systemic complications. Patient with diabetes have increased risks of bone fracture. Previous studies report that diabetes could affect bone metabolism, however, the underlying mechanism is still unclear.MethodsWe isolated exosomes secreted by bone marrow mesenchymal stem cells of normal and diabetic mice and test their effects on osteogenesis and adipogenesis. Then we screened the differential microRNAs by high-throughput sequencing and explored the function of key microRNA in vitro and in vivo.ResultsWe find that lower bone mass and higher marrow fat accumulation, also called bone-fat imbalance, exists in diabetic mouse model. Exosomes secreted by normal bone marrow mesenchymal stem cells (BMSCs-Exos) enhanced osteogenesis and suppressed adipogenesis, while these effects were diminished in diabetic BMSCs-Exos. miR-221, as one of the highly expressed miRNAs within diabetic BMSCs-Exos, showed abilities of suppressing osteogenesis and promoting adipogenesis both in vitro and in vivo. Elevation of miR-221 level in normal BMSCs-Exos impairs the ability of regulating osteogenesis and adipogenesis. Intriguingly, using the aptamer delivery system, delivery normal BMSCs-Exos specifically to BMSCs increased bone mass, reduced marrow fat accumulation, and promoted bone regeneration in diabetic mice.ConclusionWe demonstrate that BMSCs derived exosomal miR-221 is a key regulator of diabetic osteoporosis, which may represent a potential therapeutic target for diabetes-related skeletal disorders.
Comparative efficacy of aromatase inhibitors and gonadotropin-releasing hormone analogue in increasing final height of idiopathic short stature boys: a network meta-analysis
Authors: Wang Siqi , Wu Zhixin , Chen Yang , Luo Kuanhong , Cui Zhenhai , Zhang Jiaoyue , Zheng Juan , Xiao Kangli , Li Huiqing 
Published: April 2023

ObjectiveTo investigate the efficacy of monotherapy with AIs or GnRHa in improving the height of boys with idiopathic short stature (ISS).MethodWe performed a systematic search in Pubmed, The Cochrane Library, Chinese National Knowledge Infrastructure databases, and Wanfang Database for eligible studies. The network meta-analysis was conducted using STATA software.ResultsWe identified a total of four studies that included 136 individuals. We used FAH/PAH as the main outcome of final height. The results revealed a statistically higher final height after treatment with AI or GnRHa in idiopathic short stature children(MD= 4.63, 95% CI[3.29,5.96]). In network meta-analysis, the direct and indirect comparison between AI and GnRHa was presented in the forest plot. Compared with control group, both AI and GnRHa were effective in increasing the final height, with the mean effect of 4.91(95%CI:1.10,8.17) and 5.55(95%CI:1.12,9.98) respectively. However, there was no statistical difference between the GnRHa and AI treatment, of which the mean effect was 0.65(95%CI: -4.30,5.60).ConclusionBoth AIs and GnRHa monotherapy were effective in augmenting the final height of boys with idiopathic short stature when compared to placebo groups. However, there was no statistical difference between the GnRHa and AI treatments.
Inhibition of macrophages inflammasome activation via autophagic degradation of HMGB1 by EGCG ameliorates HBV-induced liver injury and fibrosis
Authors: He Minjing , Chu Tianhao , Wang Ziteng , Feng Ying , Shi Runhan , He Muyang , Feng Siheng , Lu Lin , Cai Chen , Fang Fang , Zhang Xuemin , Liu Yi , Gao Bo 
Published: April 2023

BackgroundLiver fibrosis is a reversible wound-healing response that can lead to end-stage liver diseases without effective treatment, in which HBV infection is a major cause. However, the underlying mechanisms for the development of HBV-induced fibrosis remains elusive, and efficacious therapies for this disease are still lacking. In present investigation, we investigated the effect and mechanism of green tea polyphenol epigallocatechin-3-gallate (EGCG) on HBV-induced liver injury and fibrosis.MethodsThe effect of EGCG on liver fibrosis was examined in a recombinant cccDNA (rcccDNA) chronic HBV mouse model by immunohistochemical staining, Sirius red and Masson’s trichrome staining. The functional relevance between high mobility group box 1 (HMGB1) and inflammasome activation and the role of EGCG in it were analyzed by Western blotting. The effect of EGCG on autophagic flux was determined by Western blotting and flow cytometric analysis.ResultsEGCG treatment efficiently was found to alleviate HBV-induced liver injury and fibrosis in a recombinant cccDNA (rcccDNA) chronic HBV mouse model, a proven suitable research platform for HBV-induced fibrosis. Mechanistically, EGCG was revealed to repress the activation of macrophage NLRP3 inflammasome, a critical trigger of HBV-induced liver fibrosis. Further study revealed that EGCG suppressed macrophage inflammasome through downregulating the level of extracellular HMGB1. Furthermore, our data demonstrated that EGCG treatment downregulated the levels of extracellular HMGB1 through activating autophagic degradation of cytoplasmic HMGB1 in hepatocytes. Accordingly, autophagy blockade was revealed to significantly reverse EGCG-mediated inhibition on extracellular HMGB1-activated macrophage inflammasome and thus suppress the therapeutic effect of EGCG on HBV-induced liver injury and fibrosis.ConclusionEGCG ameliorates HBV-induced liver injury and fibrosis via autophagic degradation of cytoplasmic HMGB1 and the subsequent suppression of macrophage inflammasome activation. These data provided a new pathogenic mechanism for HBV-induced liver fibrosis involving the extracellular HMGB1-mediated macrophage inflammasome activation, and also suggested EGCG administration as a promising therapeutic strategy for this disease.
Monitoring of kinetics and exhaustion markers of circulating CAR-T cells as early predictive factors in patients with B-cell malignancies
Authors: García-Calderón Clara Beatriz , Sierro-Martínez Belén , García-Guerrero Estefanía , Sanoja-Flores Luzalba , Muñoz-García Raquel , Ruiz-Maldonado Victoria , Jimenez-Leon María Reyes , Delgado-Serrano Javier , Molinos-Quintana Águeda , Guijarro-Albaladejo Beatriz , Carrasco-Brocal Inmaculada , Lucena José-Manuel , García-Lozano José-Raúl , Blázquez-Goñi Cristina , Reguera-Ortega Juan Luis , González-Escribano María-Francisca , Reinoso-Segura Marta , Briones Javier , Pérez-Simón José Antonio , Caballero-Velázquez Teresa 
Published: April 2023

PurposeCAR-T cell therapy has proven to be a disruptive treatment in the hematology field, however, less than 50% of patients maintain long-term response and early predictors of outcome are still inconsistently defined. Here, we aimed to optimize the detection of CD19 CAR-T cells in blood and to identify phenotypic features as early biomarkers associated with toxicity and outcomes.Experimental designIn this study, monitoring by flow cytometry and digital PCR (dPCR), and immunophenotypic characterization of circulating CAR-T cells from 48 patients treated with Tisa-cel or Axi-cel was performed.ResultsValidation of the flow cytometry reagent for the detection of CAR-T cells in blood revealed CD19 protein conjugated with streptavidin as the optimal detection method. Kinetics of CAR-T cell expansion in blood confirmed median day of peak expansion at seven days post-infusion by both flow cytometry and digital PCR. Circulating CAR-T cells showed an activated, proliferative, and exhausted phenotype at the time of peak expansion. Patients with increased expansion showed more severe CRS and ICANs. Immunophenotypic characterization of CAR-T cells at the peak expansion identified the increased expression of co-inhibitory molecules PD1 and LAG3 and reduced levels of the cytotoxicity marker CD107a as predictors of a better long-term disease control. ConclusionsThese data show the importance of CAR-T cells in vivo monitoring and identify the expression of PD1LAG3 and CD107a as early biomarkers of long-term disease control after CAR-T cell therapy.
Characterization of a novel Plasmodium falciparum merozoite surface antigen and potential vaccine target
Authors: Niaré Karamoko , Chege Timothy , Rosenkranz Micha , Mwai Kennedy , Saßmannshausen Zoe , Odera Dennis , Nyamako Lydia , Tuju James , Alfred Tiono , Waitumbi John N. , Ogutu Bernhards , Sirima Sodiomon B. , Awandare Gordon , Kouriba Bourema , Rayner Julian C. , Osier Faith H. A. 
Published: April 2023

IntroductionDetailed analyses of genetic diversity, antigenic variability, protein localization and immunological responses are vital for the prioritization of novel malaria vaccine candidates. Comprehensive approaches to determine the most appropriate antigen variants needed to provide broad protection are challenging and consequently rarely undertaken.MethodsHere, we characterized PF3D7_1136200, which we named Asparagine-Rich Merozoite Antigen (ARMA) based on the analysis of its sequence, localization and immunogenicity. We analyzed IgG and IgM responses against the common variants of ARMA in independent prospective cohort studies in Burkina Faso (N = 228), Kenya (N = 252) and Mali (N = 195) using a custom microarray, Div-KILCHIP.ResultsWe found a marked population structure between parasites from Africa and Asia. African isolates shared 34 common haplotypes, including a dominant pair although the overall selection pressure was directional (Tajima’s D = -2.57; Fu and Li’s F = -9.69; P &lt; 0.02). ARMA was localized to the merozoite surface, IgG antibodies induced Fc-mediated degranulation of natural killer cells and strongly inhibited parasite growth in vitro. We found profound serological diversity, but IgG and IgM responses were highly correlated and a hierarchical clustering analysis identified only three major serogroups. Protective IgG and IgM antibodies appeared to target both cross-reactive and distinct epitopes across variants. However, combinations of IgG and IgM antibodies against selected variants were associated with complete protection against clinical episodes of malaria.DiscussionOur systematic strategy exploits genomic data to deduce the handful of antigen variants with the strongest potential to induce broad protection and may be broadly applicable to other complex pathogens for which effective vaccines remain elusive.