bluuebunny/crossref_metadata_2025_split huggingface dataset:
Comparative transcriptome analysis of iron-overaccumulating Arabidopsis genotypes uncover novel putative regulators of systemic and retrograde signaling
Authors: Grillet Louis , Schmidt Wolfgang 
Published: April 2023

Abstract
Background Maintaining iron homeostasis requires a precise orchestration of cellular uptake, trafficking through mesophyll cells, translocation via xylem and phloem, and uptake of iron by roots adjusted to the demand of sink organs by systemic signals. Plants defective in the iron transporter OPT3 in the leaves behave as if they were constitutively iron deficient and accumulate toxic iron levels in their leaves. IRONMAN (IMA) genes encode peptides that trigger iron remobilization and uptake by inhibiting the ubiquitin ligase BRUTUS. Ectopic expression of IMA genes also causes iron accumulation. This study aims at bridging the knowledge gap of the mechanisms operating between OPT3-transported iron and the activation of IMA genes, and between phloem-localized IMAs and the activation of iron uptake genes in the epidermis.Results Among the 1999 DEGs identified in all the genotypes, tissues and treatments, a small subset of genes was found to be shared among the three iron-accumulating genotypes and iron-deficient control plants. Root expression profiles were more strongly correlated that those of shoots, confirming a predominant function in the regulation of root processes by IMAs and OPT3. The largest overlap was observed between opt3-2 and wild-type plants. IMA1 Ox and IMA3 Ox lined showed a surprisingly small overlap except for the iron-related genes, suggesting distinct physiological functions. The set genes with discordant expression pattern between IMA1 Ox and IMA3 Ox lines comprised approximately 40 root genes and 40 shoot genes. A small subset of genes was consistently differentially expressed in all the genotypes, including genes involved in root iron uptake and ROS detoxification, a gene encoding an F-Box protein, and two groups of non-protein coding genes.Conclusions The small suite of DEGs common to all genotypes under investigation constitute potential novel regulators of cellular iron homeostasis. A group of plastid genes seem to produce tRNA-alanine, fragments of which possibly target IMA3 transcripts, constituting a retrograde control on iron homeostasis.
Photo-click hydrogels for 3D in situ differentiation of pancreatic progenitors from induced pluripotent stem cells
Authors: Arkenberg Matthew R. , Ueda Yoshitomo , Hashino Eri , Lin Chien-Chi 
Published: April 2023

Abstract
Background Induced pluripotent stem cells (iPSC) can be differentiated to cells in all three germ layers, as well as cells in the extraembryonic tissues. Efforts in iPSC differentiation into pancreatic progenitors in vitro have largely been focused on optimizing soluble growth cues in conventional two-dimensional (2D) culture, whereas the impact of three-dimensional (3D) matrix properties on the morphogenesis of iPSC remains elusive.Methods In this work, we employ gelatin-based thiol-norbornene photo-click hydrogels for in situ 3D differentiation of human iPSCs into pancreatic progenitors (PP). Molecular analysis and single cell RNA-sequencing were utilized to elucidate on the distinct identities of subpopulations within the 2D and 3D differentiated cells.Results We found that, while established soluble cues led to predominately PP cells in 2D culture, differentiation of iPSCs using the same soluble factors led to prominent branching morphogenesis, ductal network formation, and generation of diverse endoderm populations. Through single-cell RNA-sequencing, we found that 3D differentiation resulted in enrichments of pan-endodermal cells and ductal cells. We further noted the emergence of a group of extraembryonic cells in 3D, which was absent in 2D differentiation. The unexpected emergence of extraembryonic cells in 3D was found to be associated with enrichment of Wnt and BMP signaling pathways, which may have contributed to the emergence of diverse cell populations. The expressions of PP signature genes PDX1 and NKX6.1 were restored through inhibition of Wnt signaling at the beginning of the posterior foregut stage.Conclusions To our knowledge, this work established the first 3D hydrogel system for in situ differentiation of human iPSCs into PPs. Ongoing work focuses on enhancing pancreatic differentiation efficiency through modulating physicochemical properties of the iPSC-laden matrices.
The impact of sleep quality on mental health in working Australians: A quasi-experimental approach
Authors: Foo Jia Kai , Doan Tinh 
Published: April 2023

Abstract
Work conditions such as job demand can impact individual sleep quality, and subsequently impact mental health. This study aims to investigate pathway effects of exogenous factors on mental health via sleep, and the direct effect of sleep quality on mental health in working Australians. We employ a novel method in public health studies, a quasi-experimental (instrumental variable) approach, which can address reciprocal relationships between sleep and mental health in order to estimate causal effect of sleep quality on mental health in a sample of 19,789 working Australians aged 25-64 from waves 2013, 2017, and 2021 of the Household, Income and Labour Dynamics in Australia (HILDA) survey data. We find that high job demand, as a valid instrument, adversely affects sleep quality, and the changes in sleep quality ultimately affect mental health of working Australians. These findings highlight the need for policies that address employment conditions by reducing high work demand or workload to mitigate poor sleep quality in the Australian workforce, ultimately improving workers' sleep quality and promoting better mental health, overall health outcomes and productivity.
New Framework for Identifying Discrete-Time Switched Linear Systems
Authors: Lopes Renato Vilela , Ishihara João Y. , Borges Geovany Araújo 
Published: April 2023

Abstract
This paper addresses the problem of offline identification of a particular class of hybrid dynamical systems that are discrete-time switched linear state space models. The identification process is carried out from data previously sampled from the system. Unlike most existing methods that prioritize identifying the switching instants first, a new framework is proposed in which the local models are identified first, and the task of identifying the switching instants is performed later. The methodology involves the iterative calculation of discrete and continuous models' a posteriori probability density function using subspace identification, clustering, data classification, and hybrid stochastic filtering methods. This strategy allows grouping the data most likely to have been generated by the same submodels, thus allowing the estimation of these local models. An essential feature of the algorithm is that the matrices of the different submodels are identified with the same state-space basis allowing them to be evaluated and, if necessary, combined. The performance of the identification procedure is evaluated through numerical examples, and a comparison with a prior method described in the literature is conducted.
Proteomics-driven noninvasive screening of circulating serum protein panels for the early diagnosis of hepatocellular carcinoma
Authors: Xing Xiaohua , Cai Lin-Sheng , Ouyang Jia-He , Wang Fei , Wang Ying-Chao , Zhou Yang , Hu En , Li Zong-Man , Huang Chang-Li , Wu Li-Ming , Liu Jing-Feng , Liu Xiao-Long 
Published: April 2023

Abstract
Early diagnosis of hepatocellular carcinoma (HCC) lacks highly sensitive and specific protein biomarkers. Proteomics-driven discovery of tumor biomarkers is an important direction for omics study. Here, we described a staged mass spectrometry (MS)-based discovery-verification-validation proteomics workflow to explore serum proteomic biomarkers for HCC early diagnosis in 662 individuals (373 HCC patients and 289 non-HCC patients). Our workflow reproducibly quantified 451serum proteins using a data independent acquisition mass spectrometry (DIA-MS) strategy from discovery cohort, and proteins with significantly altered abundance in HCC were validated as candidates in an independent validation cohort using targeted proteomics based on parallel reaction monitoring (PRM). Machine learning models determined as P4 serum protein-panels (two serum proteomics biomarkers: HABP2, CD163 and two clinical used serum biomarkers: AFP, PIVKA-II) could clearly distinguish HCC patients from LC patients in an independent validation cohort (AUC 0.979, sensitivity 0.925, specificity 0.915), outperforming existing clinical prediction strategies (p < 0.05). Moreover, the P4 panels showed high sensitivity in AFP negative (0.857) HCC patients and PIVKA-II negative HCC patients (0.813). Most importantly, the P4 panels were validated to be perfectly accurate in predicting the conversion of LC to HCC (accuracy: 100.0%) with predicting HCC at a median of 12.6 months prior to imaging in a prospective external validation cohort, which was superior to existing clinical prediction strategies. These results suggested that proteomics-driven serum biomarker discovery provided a valuable reference for the liquid biopsy, and had great potential to improve early diagnosis of HCC.
Assessing the influence of energy transition on economic-social growth: Case of China
Authors: Liu Pingkuo , Zhao Ruiqi , Han Xue 
Published: April 2023

Abstract
In recent years because of the one-sided interpretation of the nexus between the green & low-carbon energy transition and the high-quality economic-social growth, some over-idealized due-oriented judgments have led to failures in theories or deviations from practices. It is necessary and typical to select the panel data of 30 provinces in China from 2010 to 2020 to demonstrate that nexus, since China plays a different role in both climate change and global energy security. This paper defines and measures both the high-quality economic-social growthand the green & low-carbon energy transition efficiency. After analyzing the structure characteristics of energy transition, it also discusses the impact of the transition efficiency on the high-quality growth. The results indicate that: The inflection point from quantitative to qualitative change in the regional high-quality growth has not yet arrived, while the developed regions and the developing regions are still grappling with pronounced problems of imbalance and insufficiency. As far as now, the energy transition efficiency in the energy resource centers is much higher than that in the energy load centers. The provinces with a characteristic of energy supply-demand equilibrium have seamlessly shifted from the Introduction stage of green & low-carbon development to the Growth stage. Both the decrease in energy intensity and the increase in renewables share are able to promote the high-quality economic-social development obviously, however, the reduction of per capita carbon emission cannot yield the expected positive externality in a short run.
High expression of KIF4A predicts poor prognosis hallmark and is correlated with immune infiltrates in cervical cancer
Authors: Ma Xiaofeng , Lu Yun , Wei Bing , Wang Wenyan , Wang Enlin 
Published: April 2023

Abstract
Background Cervical cancer (CC) has become the fourth most common cancer among women and cause a larger number of deaths in worldwide. Screening at the early stage of CC is an effective precaution. Discovery of the new hallmark of CC will provide a guidance for CC screening. Kinesin family member 4A (KIF4A) expressed in a variety of tissues and also contributed to development of several cancers, however its function in CC remains unclear.Methods we download and analyzed the clinical information and mRNA profile of cervical cancer patients from TCGA and GTEx database. After normalization process, the expression values of KIF4A were calculated according to TCGA and GTEx data. We collected CC patient tissue samples from the Second Hospital of Anhui Medical University and detected KIF4A expression by IHC and WB. The immune cell infiltration analysis is preformed in the online analysis tool TIMER 2.0 (http://timer.cistrome.org/).Results The high-expression of KIF4A was demonstrated in the CC patients according to the bioinformatics analysis and clinical test. Additionally, loss-function of KIF4A with shRNA abrogated cervical cell proliferation, migration and invasion. We also found that the difference expression genes were identified between KIF4A− high and KIF4A− low CC patients among with abundant mutation of several genes occurred in the CC progression. Finally, we also proved that KIF4A was involved in the immune cell infiltration in the CC patients by clinical information analysis. These demonstrated that the dys-expression of KIF4A may be used for the CC screening and clinical therapy.
How Serious Are Bacillus Cereus Ocular Infections? -a Detrimental Effect of Undermining a Fulminant Disease: Case Report
Authors: Jeremiah Christie , Iqbal Tajunisah , Hanafi Hanidah , Keat Gan Yuen 
Published: April 2023

Abstract
Introduction
Bacillus cereus is a gram-positive endospore-forming organism. Although not commonly encountered, it can cause a consequential ocular infection leading to severe vision loss. It is usually acquired following ocular trauma. The early suspicion and detection of this organism is often overlooked in favour of more common organisms responsible for exogenous endophthalmitis such as streptococcus or klebsiella species. We aim to shed light on Bacillus Cereus endophthalmitis and the choice of suitable antibiotics that may have led to a less severe outcome.
Case Presentation
We would like to report a case of how a local farmer developed fulminant endophthalmitis following ocular trauma while ploughing his garden. Initial infection was thought to be due to commonly encountered organisms in exogenous endophthalmitis. Treatment with the standard intravenous and intravitreal antibiotics was commenced but the eye still succumbed to evisceration. The delay in diagnosis and treatment caused detrimental effect to the patient’s vision. We reviewed all other cases of Bacillus cereus-related ocular infections reported previously in the literature and analyzed their outcomes.
Conclusion
A high index of suspicion of the accountable organisms following the mechanism of injury to the eye along with timely and appropriate choice of antibiotics can lead to a better visual outcome.
Reproductive outcomes of conventional in vitro fertilization and intracytoplasmic sperm injection in patients with non-severe male infertility across poor and different sub-optimal ovarian response categories: a cohort study based on 30352 fresh cycles from 2009-2019
Authors: Jiesisibieke Dina , Tian Tian , Zhu Xiaxuan , Fang Shilin , Zhang Nan , Ma Jinxi , Xia Yuqi , Li Rong , Liu Ping , Qiao Jie , Yang Rui 
Published: April 2023

Abstract
Due to the influence of economic, social and many other factors, there are more and more reproductive problems. Originally introduced for managing male factor infertility, intracytoplasmic sperm injection had become the most commonly used fertilization treatment in the world, with broadened indications including low oocyte yield, prior fertilization failure with conventional in vitro fertilization etc. However, academic evidence for better live-birth outcomes of intracytoplasmic sperm injection over conventional in vitro fertilization is limited. Thus, we aimed to compare the reproductive outcomes of conventional in vitro fertilization and intracytoplasmic sperm injection in patients with non-severe male factor infertility across poor and different sub-optimal ovarian response categories. The fertility rate, implantation rate, clinical pregnancy rate, live birth rate and other obstetric outcomes were mainly compared. Our results showed that independent of the number of oocytes retrieved, intracytoplasmic sperm injection significantly increased the fertilization rate, while conventional in vitro fertilization cycles showed a higher implantation rate, clinical pregnancy rate, and live birth rate. No differences were observed in most obstetric outcomes. Our study indicates that poor ovarian response is not an indication for intracytoplasmic sperm injection in couples with non-severe male infertility.
Comparison of the effects of isokinetic and isotonic strength training in team sport athletes: A randomized controlled trial
Authors: Eyre Julia Christine , Weyer Jonas , Geisler Stephan , Gavanda Simon 
Published: April 2023

Abstract
Background Athletes may benefit from isokinetic training (IK) to improve strength and jump performance. However, previous studies comparing IK methods to isotonic resistance training (IT) have utilized high-repetition protocols and a Dynamometer, which are usually reserved for laboratory testing or rehabilitation settings.Methods This study compared the effects of IK and IT using ≤ 6 repetitions on strength and jump performance in 50 male, team-sport athletes (ages 18–35) during COVID-19 lockdown using the commercially-available TechnoGym BioCircuit Dynamometer. With statistical significance set to p < 0.05, this 4-group randomized parallel study assessed the effects of an 8-week isokinetic at 80°/s (IK80; n = 16) and 25°/s (IK25; n = 12) or isotonic training program (IT; n = 15) compared to a control group (CG; n = 7) on anthropometric measurements, peak torque at 80°/s (PT80) and 25°/s (PT25), one-repetition maximum (1-RM) leg extension, and countermovement (CMJ) and squat jump height.Results BM, FFM, FM, and TC were not different between groups post intervention. Strength increased in IT (PT80: p = 0.006; PT25: p = 0.023; 1-RM: p = 0.001), IK80 (PT80: p = 0.004; PT25: p = 0.021; 1-RM: p = 0.009), and IK25 (PT80: p = 0.042; PT25: p = 0.048; 1-RM: p = 0.009) compared to CG, with no difference between intervention groups (p = 1.000). SJ increased in all intervention groups (IT: +1.5cm, p = 0.036; IK80: +2.7cm, p < 0.001; IK25: +1.9cm, p = 0.021) compared to the CG (CG-IT: p = 0.005; CG-IK80: p = 0.009; CG-IK25: p = 0.004), with difference between groups (all p = 1.000). CMJ improved only in IT (+ 1.4cm, p = 0.035) and IK80 (+ 2.7cm, p < 0.001) with no difference between groups (p = 1.000).Conclusions Low-repetition IK and IT can be effectively used to improve strength and jump performance. However, the efficacy of IK on CMJ depends on training velocity and repetition count, with higher velocity training protocols (80°/s) being more effective for jump performance and lower speeds potentially more useful for improving maximal strength (25°/s).
Unveiling the molecular identity of the diminutive cyprinid, Horadandia brittani (Teleostei: Cyprinidae), a species endemic to Southern India
Authors: Daniel N. , Nanaobi Hemam , Praveenraj J. , Balaji V. , Kumar J. Stephen Sampath 
Published: April 2023

Abstract
Horadandia brittani is a small cyprinid fish species found in the coastal floodplains of southern India. For almost 50 years, the Horadandia genus was monotypic with only one species (Horadandia atukorali) confined to Sri Lanka until the discovery of H. brittani in south-western India in 1992. Despite being described as a separate species, H. brittaniwas later considered a synonym of H. atukorali until 2013 when researchers recognized it as a distinct species based on some morphological differences. However, no one had yet generated DNA sequences to validate the identity of H. brittani and its evolutionary relationship with its closely related species. To address this gap, this study was conducted to generate DNA sequences and validate the identity of H. brittani using molecular data. The results showed that H. brittani is genetically distinct from H. atukorali with mitochondrial cytochrome C oxidase (COI) gene distance values ranging from 3.21-3.63%. The study also established the phylogenetic relationships between these two species, confirming H. brittani as a valid species based on COI gene sequences. These sequences can be used to identify H. brittani quickly and accurately in the future.
Bullying and Victimization among Students with Disabilities in Higher Education, United Arab Emirates
Authors: sallam ahmed , alomosh ahmad , Al-Othman Hussain , alsaadi asma 
Published: April 2023

Abstract
Regional and national level data on bullying victimization and its associated factors among students with disabilities are still lacking, particularly in higher education. Therefore, the current study aimed to conduct a random survey of a sample of students with disabilities in university education (n = 135) with different disabilities (physical, visual, hearing impairments, learning difficulties). Using the (bullying scale) prepared by the researchers and its stability and validity were calculated, multivariate analyzes of the data showed that the prevalence of bullying students with disabilities in university education in the UAE community is (40%), that (60%) are not parties to the bullying incident, and the percentage (36.3%) %) are the victims of bullying, and the percentage (3%) is the party that caused the bullying. The most common place in which bullying occurs is the bathrooms (16%), followed by the hallway (16%), then the classroom (14%). The most unsafe places are bathrooms (30%), followed inside the classroom (17%), followed by hallways (15%). The most common forms of bullying are nicknames and bad insults (27%), followed by neglect and exclusion (percentage). 22%, followed by threats (13%), cyberbullying (12%), physical bullying (11%), and theft or destruction of personal property (11%). The most common motive for bullying is weakness or disability (31%), followed by height (13%), young age (10%), and financial circumstances (5%). In addition to other results related to the age of the bully, his size, his relationship with the victim, the most prominent forms of bullying practiced by students with disabilities themselves as a response to bullying, and the extent to which teachers are aware of the occurrence of bullying, and the respondents suggested a set of proposals to reduce bullying in universities, Such as immediate reporting of bullying cases (34%), followed by effective academic counseling (30%), followed by continuous parental supervision (26%), awareness-raising (7%) and strict punishment (3%).
Hepatosplenic Schistosomiasis-Associated Chronic Portal Vein Thrombosis: Risk Factor for Hepatocellular Carcinoma
Authors: Darce George Felipe Bezerra , Makdissi Fabio Ferrari , Ando Sabrina Mello , Widman Azzo , Fonseca Gilton Marques , Kruger Jaime Arthur Pirola , Coelho Fabricio Ferreira , Rocha Manoel de Souza , Herman Paulo 
Published: April 2023

Abstract
Background: Hepatosplenic schistosomiasis is an endemic disease prevalent in tropical countries and is associated with a high incidence of portal vein thrombosis (PVT). Inflammatory changes caused by both parasitic infection and portal thrombosis can lead to the development of chronic liver disease with potential carcinogenesis. This study aimed to assess the incidence of PVT and hepatocellular carcinoma (HCC) in patients with schistosomiasis during a long-term follow-up.
Methods: A retrospective study was conducted involving patients with schistosomiasis followed up at our institution between 1990 and 2021.
Results: A total of 126 patients with schistosomiasis were evaluated in the study. The mean follow-up time was 16 years (range 5–31). Of the total, 73 (57.9%) patients presented PVT during the follow-up. Six (8.1%) of them were diagnosed with HCC, all of them with portal vein thrombosis diagnosed more than 10 years before.
Conclusion: The incidence of HCC in patients with schistosomiasis and chronic PVT highlights the importance of a systematic long-term follow-up in this group of patients.