bluuebunny/crossref_metadata_2025_split huggingface dataset:
HTLV-1 bZIP Factor-Induced Reprogramming of Lactate Metabolism and Epigenetic Status Promote Leukemic Cell Expansion
Authors: Toyoda Kosuke , Yasunaga Jun-ichirou , Shichijo Takafumi , Arima Yuichiro , Tsujita Kenichi , Tanaka Azusa , Salah Tarig , Zhang Wenyi , Hussein Osama , Sonoda Miyu , Watanabe Miho , Kurita Daisuke , Nakashima Kazutaka , Yamada Kyohei , Miyoshi Hiroaki , Ohshima Koichi , Matsuoka Masao 
Published: May 2023

Abstract

Acceleration of glycolysis is a common trait of cancer. A key metabolite, lactate, is typically secreted from cancer cells because its accumulation is toxic. Here, we report that a viral oncogene, HTLV-1 bZIP factor (HBZ), bimodally upregulates TAp73 to promote lactate excretion from adult T-cell leukemia-lymphoma (ATL) cells. HBZ protein binds to EZH2 and reduces its occupancy of the TAp73 promoter. Meanwhile, HBZ RNA activates TAp73 transcription via the BATF3-IRF4 machinery. TAp73 upregulates the lactate transporters MCT1 and MCT4. Inactivation of TAp73 leads to intracellular accumulation of lactate, inducing cell death in ATL cells. Furthermore, TAp73 knockout diminishes the development of inflammation in HBZ-transgenic mice. An MCT1/4 inhibitor, syrosingopine, decreases the growth of ATL cells in vitro and in vivo. MCT1/4 expression is positively correlated with TAp73 in many cancers, and MCT1/4 upregulation is associated with dismal prognosis. Activation of the TAp73–MCT1/4 pathway could be a common mechanism contributing to cancer metabolism.

Significance:
An antisense gene encoded in HTLV-1, HBZ, reprograms lactate metabolism and epigenetic modification by inducing TAp73 in virus-positive leukemic cells. A positive correlation between TAp73 and its target genes is also observed in many other cancer cells, suggesting that this is a common mechanism for cellular oncogenesis.
This article is featured in Selected Articles from This Issue, p. 337
Culinary Diaspora: An Anchor for Indonesian Gastrodiplomacy in the Netherlands
Authors: Yayusman Meilinda Sari , Mulyasari Prima Nurahmi , Trihartono Agus , Gusnelly†  
Published: May 2023

Summary
Many Indonesian diasporas in the Netherlands have established culinary businesses for both economic purposes and out of a sense of nostalgia for their home country by emphasising Indonesian food authenticity and the richness of spices in their new home. The Netherlands, with its strong historical links with Indonesia, plays a pivotal role in Indonesian gastrodiplomacy. However, the Indonesian government has not yet paid attention to the importance of harnessing the existence of diasporas and targeting the Netherlands as a strategic country of implementation in the context of strengthening Indonesian gastrodiplomacy. This article suggests that Indonesian diasporas, through representation in restaurants, could be a ‘frontline messenger’ in introducing Indonesian foods to the Netherlands and be the anchor of gastrodiplomacy. While Indonesia is now strategising gastrodiplomacy, diasporas are of the utmost importance in winning the hearts and minds of the public.
Soil Moisture Influences on Summer Arctic Cyclones and Their Associated Poleward Moisture Transport
Authors: Fearon Matthew G. , Doyle James D. , Finocchio Peter M. 
Published: May 2023

Abstract
Moisture transport into the Arctic is an important modulator for clouds, radiative forcing, and sea ice change. Transport events—namely, moist-air intrusions—are often associated with Arctic cyclones, and during the summer season we find that the high-latitude land surface is a significant moisture source for intrusions. Summer Arctic cyclones typically originate from the surrounding continental interior and shorelines where during the early stages of intensification the warm sector experiences strong latent heat fluxes from the land surface. In this study, we use multiyear reanalysis data and back-trajectory calculations to quantify the linkages between key continental moisture source regions and water vapor within cyclone-induced intrusions. We also conduct regional soil moisture sensitivity experiments using the Coupled Ocean–Atmosphere Mesoscale Prediction System (COAMPS) to diagnose the land surface moisture contribution for an August 2016 Arctic cyclone case. Results from reanalysis show that land regions on average account for more than 30% of the total moist-air intrusion flux at 70°N during summer. COAMPS case-study experiments reaffirm this result, showing that land surface moisture flux on average accounts for 30% of the intrusion water vapor content. COAMPS experiments further reveal that land surface moisture impacts cyclone intensification and moist-air intrusion cloud water vapor. When the regional soil moisture is reduced, intrusion cloud cover is also reduced, resulting in an increase in the surface solar radiation > 90 W m−2. These results demonstrate that the high-latitude land surface plays an important role in the Arctic summer hydrological cycle and may be increasingly impactful as traditionally cold or frozen soils warm.

Significance Statement
The purpose of this study is to better understand to what extent high-latitude land surface moisture flux is entrained into summer Arctic cyclones and their poleward moisture transport. Moisture transport into the Arctic is important for regional clouds, radiative forcing, and sea ice change. Our results show that land surface evaporation augments cyclone-induced moisture transport into the Arctic on average by 30%. Results are important because traditionally cold or partially frozen high-latitude soils continue to warm and exhibit more positive evaporative trends, including areas undergoing permafrost thaw.
The Tertiary Survey as a Quality Improvement Initiative in Pediatric Trauma Care
Authors: Ulloa Emily , Archie Jessica , Slevakumar Sruthi , Levy Marc , Elkbuli Adel , Plumley Donald 
Published: May 2023

Background Patients are at risk of missed or delayed injuries in the setting of multisystem trauma, which may be identified with a tertiary trauma survey (TTS). There is limited literature to support the utilization of a TTS in pediatric trauma population. We aim to assess the impact of the TTS as a quality and performance improvement tool in identifying missed or delayed injuries and improving the quality of care among pediatric trauma population. Methods A retrospective study assessing a quality improvement/performance improvement (QI/PI) project focusing on the administration of tertiary surveys to pediatric trauma patients was conducted at our level 1 trauma center between 08-2020 and 08-2021. Patients with injury severity scores (ISS) greater than 12 and/or an anticipated hospital stay greater than 72 hours met inclusion criteria and were included. Results Of the 535 trauma patients admitted to the pediatric trauma service during the study period, 85 (16%) patients met the criteria and received a TTS. Thirteen unaddressed or undertreated injuries were found in 11 patients: 5 cervical spine injuries, 1 subdural hemorrhage, 1 bowel injury, 1 adrenal hemorrhage, 1 kidney contusion, 2 hematomas, and 2 full thickness abrasions. Following TTS, 13 patients (15%) had additional imaging, which identified 6 of the 13 injuries. Conclusion The TTS is a valuable quality and performance improvement tool in the comprehensive care of trauma patients. Standardization and implementation of a tertiary survey have the potential to facilitate the prompt detection of injuries and improve the quality of care for pediatric trauma patients. Level of Evidence III.
Secondary mental health service utilisation following emergency department contact for suicidal behaviour: A systematic review
Authors: Feng YR , Valuri GM , Morgan Vera A , Preen DB , O’Leary Colleen M , Crampin E , Waterreus A 
Published: May 2023

Objective: Engagement with secondary mental health services after an emergency department presentation with suicidal behaviours may be an important strategy for reducing the risk of repeat attempts. Our aim was to examine secondary mental health service contact following a presentation to emergency department with suicidal behaviours. Methods: A systematic review of papers published between 2000 and 2020 was undertaken. This identified 56 papers relating to 47 primary studies. Data were extracted and summarised separately by age group: (1) young people, (2) older adults and (3) adults and studies with participants of ‘all ages’. Results: Studies in young people ( n = 13) showed, on average, 44.8% were referred and 33.7% had contact with secondary mental health services within 4 weeks of emergency department discharge. In comparison, in adult/all ages studies ( n = 34), on average, 27.1% were referred to and 26.2% had mental health service contact within 4 weeks. Only three studies presented data on contact with mental health services for older adults, and proportions ranged from 49.0% to 86.0%. Conclusion: This review highlights poor utilisation of secondary mental health service following emergency department presentation for suicidal behaviours, and further research is needed to identify the reasons for this. Crucially, this information could assist in the allocation of resources to facilitate the timely implementation of suicide prevention services.
Youth voices creating healthy eating and physically active environments in schools
Authors: Shah Smita , Rizzo Liu Kym , Lockett Catriona , Sainsbury Emma 
Published: May 2023

Objective: The objectives of this study were to (1) measure the acceptability of Students As LifeStyle Activists (SALSA) Youth Voices (SYV) programme, (2) determine the skills gained by peer leaders from their participation in SYV and (3) determine whether peer leaders were able to design and implement an action to promote healthy eating and physical activity in their school. Design: Process evaluation. Setting: Peer leaders (aged 15–16 years) from high schools in Western Sydney, Australia. Method: We conducted a process evaluation to determine the reasons why students participated in SYV, which workshop activities they enjoyed, the skills they gained from participating in SYV and whether the actions were designed and implemented. Peer leaders completed an online survey at the end of the Leadership Day. The peer leaders then presented their actions at an SYV Action Day event and completed a further online survey. Results: Eighty-four peer leaders from seven high schools in Western Sydney participated in SYV. Peer leaders reported their involvement with the SYV programme as positive, with 68% rating it as ‘very valuable’. Skills gained by the peer leaders included teamwork (90%), communication (85%), leadership (77%) and confidence (65%). Peer leaders planned and devised actions, which included installing water refill stations, fitting the school gymnasium with new equipment, redesigning girls’ sports shorts, and other strategies to engage girls in physical activity. Students from all seven schools presented their actions to receive feedback and expressed confidence that their actions were sustainable. Conclusion: The SYV programme showed that providing peer leaders with ownership of their actions was successful in creating healthy school environments, while building leadership, teamwork, communication skills and confidence in adolescents.
Trajectory of PaO2/FiO2 Ratio in Shock After Angiotensin II
Authors: Wieruszewski Patrick M. , Coleman Patrick J. , Levine Andrea R. , Davison Danielle , Smischney Nathan J. , Kethireddy Shravan , Guo Yanglin , Hecht Jason , Mazzeffi Michael A. , Chow Jonathan H. 
Published: May 2023

Introduction High-dose catecholamines can impair hypoxic pulmonary vasoconstriction and increase shunt fraction. We aimed to determine if Angiotensin II (Ang-2) is associated with improved PaO2/FiO2 and SpO2/FiO2 in patients in shock. Methods Adult patients at four tertiary care centers and one community hospital in the United States who received Ang-2 from July 2018-September 2020 were included in this retrospective, observational cohort study. PaO2, SpO2, and FiO2 were measured at 13 timepoints during the 48-h before and after Ang-2 initiation. Piecewise linear mixed models of PaO2/FiO2 and SpO2/FiO2 were created to evaluate hourly changes in oxygenation after Ang-2 initiation. The difference in the proportion of patients with PaO2/FiO2 ≤ 300 mm Hg at the time of Ang-2 initiation and 48 h after was also examined. Results The study included 254 patients. In the 48 h prior to Ang-2 initiation, oxygenation was significantly declining (hourly PaO2/FiO2 change −4.7 mm Hg/hr, 95% CI − 6.0 to −3.5, p < .001; hourly SpO2/FiO2 change −3.1/hr, 95% CI−3.7 to −2.4, p < .001). Ang-2 treatment was associated with significant improvements in PaO2/FiO2 and SpO2/FiO2 in the 48-h after initiation (hourly PaO2/FiO2 change +1.5 mm Hg/hr, 95% CI 0.5-2.5, p  =  .003; hourly SpO2/FiO2 change +0.9/hr, 95% CI 0.5-1.2, p < .001). The difference in the hourly change in oxygenation before and after Ang-2 initiation was also significant ( pinteraction < 0.001 for both PaO2/FiO2 and SpO2/FiO2). This improvement was associated with significantly fewer patients having a PaO2/FiO2 ≤ 300 mm Hg at 48 h compared to baseline (mean difference −14.9%, 95% CI −25.3% to −4.6%, p  =  .011). Subgroup analysis found that patients with either a baseline PaO2/FiO2 ≤ 300 mm Hg or a norepinephrine-equivalent dose requirement >0.2 µg/kg/min had the greatest associations with oxygenation improvement. Conclusions Ang-2 is associated with improved PaO2/FiO2 and SpO2/FiO2. The mechanisms for this improvement are not entirely clear but may be due to catecholamine-sparing effect or may also be related to improved ventilation-perfusion matching, intrapulmonary shunt, or oxygen delivery.
Unsteady flow of hybrid fluid via peristaltic pumping in wavy micro-channel under electric and magnetic field effects: An application of Hall device and slip boundary
Authors: Bilal Muhammad , Javid Khurram , Ali Nasir 
Published: May 2023

Aim of study In the current analysis, the blood is used as a viscoelastic fluid and manipulated with an electromagnetic field because of its magnetic features. Furthermore, blood is considered as a non-Newtonian fluid that is productive in nano- and micro-scales transport. To study the performance of hybrid fluids, a selection of nanofluids is examined with a Jeffrey fluid (blood) which is doped with [Formula: see text] and [Formula: see text] nanoparticles. Mathematical formulation The long wavelength assumption, lubrication theory and Debye–Huckel linearization are employed. Analytical solutions of flow equations are implemented in Mathematica by using integration technique. The impacts of considered parameters on the flow are computed. Combined influence of Hall device, momentum, and thermal slips is observed on various flow features. Outcomes Magnitude of the velocity goes on decreasing by increasing magnetic and viscoelastic parameters. The wavy behavior is predicted in the graphs of pressure gradient due to the complex nature of peristaltic walls. Enhancing the viscoelastic effects and concentration of nanoparticles results in reduction of magnitude of the pressure gradient. Heat transfer of hybrid fluid is larger in steady state flow as compared to the unsteady state. The heat source/sink parameter has remarkable impacts on enhancement of heat transfer phenomena of viscoelastic hybrid fluid. Applications The present investigation is relevant to electromagnetic hybrid liquid micropump manufactures and emerging nano-physiological technologies.
Microsomal glutathione S-transferase 1 targets the autophagy signaling pathway to suppress ferroptosis in gastric carcinoma cells
Authors: Peng Z , Peng N 
Published: May 2023

Objective Ferroptosis is a newly discovered form of programmed cell death; however, the specific mechanisms that regulate ferroptosis have yet to be fully elucidated in gastric carcinoma. In this study, we aimed to investigate how microsomal glutathione S-transferase 1 (MGST1) regulates ferroptosis in gastric carcinoma cells. Methods Gastric adenocarcinoma (SGC7901) cells that overexpressed MGST1 or expressed only low levels of MGST1, were treated with specific compounds (erastin, sorafenib, RSL3, MK-2206 and SC79). Then, we detected the levels of malondialdehyde (MDA), glutathione (GSH), iron and reactive oxygen species (ROS). Protein expression levels of the non-classical autophagy and protein kinase B (Akt)/glycogen synthase kinase-3β (GSK-3β) pathways were determined by western blotting and cell viability was analyzed by Cell Counting Kit-8 (CCK-8) assays. The expressions of target genes were detected using qRT-PCR. Results We evaluated a range of ferroptosis-inducing compounds and found that MGST1 expression was down-regulated during ferroptosis in SGC7901 cells. The ferroptosis inducer RSL3 played a role in classical ferroptotic events while the overexpression of MGST1 impaired these effects. Interestingly, the overexpression of MGST1 resulted in the inactivation of autophagy by repressing the expression of ATG16L1 and the conversion of LC3-I to LC3-II. The upregulation of ATG16L1 eliminated the inhibitory action of MGST1 on ferroptosis. Notably, the overexpression of MGST1 induced the activation of the Akt/GSK-3β pathway. An Akt inhibitor antagonized the inhibitory effects of MGST1 on autophagy and ferroptosis. Conclusion Collectively, our findings demonstrate a novel molecular mechanism and signaling pathway for ferroptosis. We also characterized that the overexpression of MGST1 induces gastric carcinoma cell proliferation by activating the Akt/GSK-3β signaling pathway.
Single-cell Transcriptome Landscape of DNA Methylome Regulators Associated with Orofacial Clefts in the Mouse Dental Pulp
Authors: Enkhmandakh Badam , Joshi Pujan , Robson Paul , Vijaykumar Anushree , Mina Mina , Shin Dong-Guk , Bayarsaihan Dashzeveg 
Published: May 2023

Objective Significant evidence links epigenetic processes governing the dynamics of DNA methylation and demethylation to an increased risk of syndromic and nonsyndromic cleft lip and/or cleft palate (CL/P). Previously, we characterized mesenchymal stem/stromal cells (MSCs) at different stages of osteogenic differentiation in the mouse incisor dental pulp. The main objective of this research was to characterize the transcriptional landscape of regulatory genes associated with DNA methylation and demethylation at a single-cell resolution. Design We used single-cell RNA sequencing (scRNA-seq) data to characterize transcriptome in individual subpopulations of MSCs in the mouse incisor dental pulp. Settings The biomedical research institution. Patients/Participants This study did not include patients. Interventions This study collected and analyzed data on the single-cell RNA expssion in the mouse incisor dental pulp. Main outcome measure(s) Molecular regulators of DNA methylation/demethylation exhibit differential transcriptional landscape in different subpopulations of osteogenic progenitor cells. Results scRNA-seq analysis revealed that genes encoding DNA methylation and demethylation enzymes (DNA methyltransferases and members of the ten-eleven translocation family of methylcytosine dioxygenases), methyl-DNA binding domain proteins, as well as transcription factors and chromatin remodeling proteins that cooperate with DNA methylation machinery are differentially expressed within distinct subpopulations of MSCs that undergo different stages of osteogenic differentiation. Conclusions These findings suggest some mechanistic insights into a potential link between epigenetic alterations and multifactorial causes of CL/P phenotypes.
COVID-19 Vaccination Clinic for Individuals With Autism Spectrum Disorder and Related Disorders: Feasibility and Acceptability
Authors: Pavlov Alexis , Hodnett Jennifer M. , Booth Chris , Wigton Sarah , Bernstein Alec , Lomas Mevers Joanna , Scheithauer Mindy 
Published: May 2023

Introduction: Autistic individuals often have comorbid medical conditions, which can increase the likelihood of being severely affected by COVID-19. The best prevention for this is vaccination. However, some autistic individuals engage in behaviors that might create a barrier to successful vaccination. Aims: We describe the development and acceptability of a clinic specializing in the administration of COVID-19 vaccines for individuals diagnosed with autism spectrum disorder (ASD) who exhibit challenging behavior. Method: The clinic utilized behavioral antecedent strategies and contingencies to increase compliance with the vaccine and decrease distress associated with the procedure. Results: We achieved a 100% success rate with vaccine administration and caregivers reported high satisfaction with the clinic. Conclusions: The present vaccine clinic is adaptable to various settings and patients and was well-received overall by caregivers. Replication in different geographic regions may be beneficial when resources allow for this type of clinic.
Anatomical variations of the great saphenous vein at the saphenofemoral junction. A cadaveric study and narrative review of the literature
Authors: Tepelenis Kostas , Papathanakos Georgios , Kitsouli Aikaterini , Barbouti Alexandra , Varvarousis Dimitrios N , Kefalas Athanasios , Anastasopoulos Nikolaos , Paraskevas Georgios , Kanavaros Panagiotis 
Published: May 2023

Objectives The great saphenous vein is the lower limb’s longest and thickest-walled superficial vein. Its anatomy is complex, while its anatomical variations are widespread. This study aimed to illustrate the anatomy and variations of the great saphenous vein at the saphenofemoral junction. Methods The study was conducted on 75 fresh-frozen cadaveric lower limbs. Cadavers with evidence of prior leg surgery or trauma and any congenital abnormality or gross deformity were excluded. The saphenofemoral junction was studied in detail with emphasis on the number, incidence, draining pattern of tributaries, and duplication of the great saphenous vein. Results Out of the 75 cadaveric lower limbs, 57.3% were obtained from males, and 42.7% were obtained from females. The mean age of the cadaveric lower extremities was 66.6 years (range 42–91). The number of tributaries at the saphenofemoral junction varied from 0 to 7, with a mean of 3.8. The most frequent number of branches was 4–5 in 61.3% of cases. The most consistent tributary was the superficial external pudendal vein, while the posterior accessory great saphenous vein was the least frequent tributary. 80% of the branches drained into the saphenofemoral junction directly (53.3%) or by a common trunk (26.7%%). The most frequent common trunk was the superficial epigastric and superficial circumflex iliac vein (10.7%). The rest 20% of the tributaries drained directly into the common femoral vein. The commonest branch reaching the common femoral vein was the superficial external pudendal vein (10.7%). Duplication of the great saphenous vein was observed in 2.7% of the cases. Conclusions There is a significant variation of tributaries at the saphenofemoral junction regarding the number, incidence, draining pattern of branches, and duplication of the great saphenous vein.