bluuebunny/crossref_metadata_2025_split huggingface dataset:
Hygiene Practices and Associated Factors Among Meat Handlers at Butcher Houses and Restaurants in Gambela Town, Southwest Ethiopia
Authors: Gil Ruot Gatwech , Siraj Shuayib Shemsu , DONACHO DEREJE OLJIRA 
Published: February 2023

Abstract
Background
Meat is subjected to contamination from a variety of sources during slaughter and sale. Globally, an estimated 600 million people per year are affected by illness from poor meat handling, of which 420,000 result in deaths. However, evidence on the magnitude and factors associated with hygienic practice in low-income countries limits the extent of this underestimation of the problem. The aim of this study was to assess the magnitude of meat hygiene practices and factors associated with poor practices among food handlers in Gambela, Ethiopia.
Methods
This institution-based cross-sectional study was conducted in Gambela town. Systematic sampling at random was used to select workers from butchers’ houses, meat-selling shops, and restaurants in Gambela. A sampling frame of 1,080 workers was obtained from the total number of restaurants, meat-selling shops, and restaurants in the town. A sampling interval (K) was calculated. Finally, a participant was selected for every two individuals until the final 422 individuals were sampled. Data were collected using structured questions through interviews and observations. Data collectors were trained and oriented on the questionnaires before data collection, and they were supervised throughout the data collection period.
Results
This study showed a low prevalence of good hygiene practices of 40.6% (36.0–45.6) among meat handlers at butcher houses and restaurants in Gambela, southwest Ethiopia. Low levels of good hygiene practices were significantly related to education (i.e., having a secondary education) (AOR: 95% C.I., 2.51: 1.17–5.41) and food hygiene training (i.e., receiving training on general safe food hygiene) (AOR: 95% C.I., 5.11: 2.55–10.23).
Conclusion
This study finds evidence of a low level of hygiene practices among meat handlers at butcher houses and restaurants in Gambela, Ethiopia. Low hygiene practices were significantly associated with the education level of food handlers and meat hygiene training. Intensive food safety training and providing a comfortable work environment will help them attract educated workers.
Effectiveness assessment of paraloid B-72 enhanced with Nano materials to improve of mortars properties for conservation of Seti I temple in Al-Qurna, Thebes west bank, Egypt
Authors: Mohamed Essam H. 
Published: February 2023

Abstract
This paper presents an experimental study to assessment of the potential effectiveness of four consolidation treatments performed with: Nano silica 5% dissolved in water, Nano lime 5% dissolved in ethanol, Paraloid B72 3% dissolved in toluene enhanced with Nano silica 5%, and Paraloid B72 3% dissolved in toluene enhanced with Nano lime 5% to improve the physical and mechanical properties of proposed completion mortars that will use to compensation of the missing parts of the sandstone. The experimental samples were exposed to artificial ageing cycles and investigated by several scientific techniques such as; X-Ray Diffraction (XRD), Polarized light microscope (PLM), Scanning Electron Microscopy (SEM) coupled with X-ray energy dispersive system (EDS), and the physical and mechanical properties tests to determine the appropriate mortar for the completion process. Results of this study indicated that the mortar which consolidated by 3% of paraloid B72 enhanced with 5% of Nano silica and 3% of paraloid B72 enhanced with 5% of Nano lime achieved the best results.
Use of life cycle assessment for estimating impacts of waste-to-energy technologies in solid waste management systems: The case of Buenos Aires, Argentina
Authors: Galván S. Luciana , Bielsa Raquel Ofelia 
Published: February 2023

Abstract
Analysing municipal solid waste (MSW) management scenarios is relevant for planning future policies and actions toward a circular economy. Life cycle assessment (LCA) is appropriate for evaluating technologies of MSW treatment and their environmental impacts. However, in developing countries, advanced assessments are difficult to introduce due to the lack of technical knowledge, data, and financial support. This research aims to assess the main potentialities of the introduction of waste-to-energy (WtE) systems in a developing Argentinean urban area taking into account the existing regulations about MSW recycling goals. The study was conducted with WRATE software and the proposed scenarios were: current management, grate incineration of raw MSW, incineration of solid recovered fuel (SRF). In addition, a sensitivity analysis on the energy matrix was included. It was found that the production of SRF allows increasing the energy generation from waste by 200% and reducing the environmental impact of about 10% regarding the current MSW management system. Acidification Potential and Abiotic Depletion Potential were sensitive to changes in electricity mix. Results showed that if MSW reduction goals are achieved, changes in MSW composition will affect the performance of WtE plants and, in some cases, they will be not technically feasible. The outcomes of this study can be of interest for developing countries stakeholders, and practitioners interested in LCA and sustainable MSW management.
c-MYC-dependent transcriptional inhibition of autophagy is implicated in Cisplatin sensitivity in HPV-positive Head and Neck Cancer
Authors: Medda Alessandro , Spini Giorgio , Compagnoni Micaela , Citro Simona , Croci Ottavio , Campaner Stefano , Tagliabue Marta , Ansarin Mohssen , Chiocca Susanna 
Published: February 2023

Abstract
Autophagy is important for removal, degradation and recycling of damaged organelles, proteins, and lipids through the degradative action of lysosomes. In addition to its catabolic function, autophagy is important in cancer and viral-mediated tumorigenesis, including Human Papillomavirus (HPV) positive cancers. HPV infection is a major risk factor in a subset of Head and Neck Cancer (HNC), for which no targeted therapies are currently available. Herein, we assessed autophagy function in HPV-positive HNC. We showed that HPV-positive HNC cells presented a transcriptional and functional impairment of autophagic process compared to HPV-negative cells, which was reactivated by knocking-down HPV E6/E7 oncoproteins, the drivers of cellular transformation. We found that the oncoprotein c-MYC was stabilized and triggered in HPV-positive cell lines. This resulted in the reduced binding of the MiT/TFE transcription factors to their autophagy targets due to c-MYC competition. Thus, knock-down of c-MYC induced the upregulation of autophagic and lysosomal genes in HPV-positive HNC cells, as well as the increase of autophagic markers at the protein level. Moreover, HPV oncoprotein E7 upregulated the expression of the phosphatase inhibitor CIP2A, accounting for c-MYC upregulation and stability in HPV + HNC cells. CIP2A mRNA expression negatively correlated with autophagy gene expression in tumor tissues from HNC patients, showing, for the first time, its implication in a transcriptional autophagic context. Both CIP2A and c-MYC knock-down, as well as pharmacological downregulation of c-MYC, resulted in increased resistance to Cisplatin treatment. Our results not only show a novel way by which HPV oncoproteins manipulate the host machinery, but also provide more insights on the role of autophagy in chemoresistance, with possible implications for targeted HPV-positive HNC therapy.
Realizing tight-binding Hamiltonians using site-controlled coupled cavity arrays
Authors: Majumdar Arka , Saxena Abhi , Manna Arnab , Trivedi Rahul 
Published: February 2023

Abstract
Analog quantum simulators rely on programmable quantum devices to emulate Hamiltonians describing various physical phenomenon. Photonic coupled cavity arrays are a promising platform for realizing such devices. Using a silicon photonic coupled cavity array made up of \(8\) high quality-factor resonators and equipped with specially designed thermo-optic island heaters for independent control of cavities, we demonstrate a programmable device implementing tight-binding Hamiltonians with access to the full eigen-energy spectrum. We report a \(\tilde50\%\) reduction in the thermal crosstalk between neighboring sites of the cavity array compared to traditional heaters, and then present a control scheme to program the cavity array to a given tight-binding Hamiltonian.
Documentation of phytochemicals, bio-toxicity and haemato-physiological responses at sub-lethal concentrations of Parthenium hysterophorus hydroethanolic extract in Cyprinus carpio: a concept of waste valorization as a phyto-ichthyotoxin
Authors: KHAN MD. IDRISH RAJA , Sharma Monika , Sonkar Parasmani , Komre Priyanka , Kumar Shashank , Barik Pabitra 
Published: February 2023

Abstract
In the present study, phytoextraction was performed through aqueous, alcoholic and hydroethanolic (80%) solvents followed by phytochemical profiling and evaluation of median lethal concentration (LC50) of hydroethanolic extract in Common carp, Cyprinus carpio. The haemato-immunological and biochemical response was evaluated at sub-lethal concentrations (T1: 0.395 mg l-1 and T2: 0.79 mg l-1 and control: devoid of extract) at three intervals (24, 48 and 96 h). The study revealed the presence of toxic constituents in extracts namely saponin, rotenone, tannin and alkaloid. Moreover, phytochemical typing exhibited the superior ability of hydroethanolic solvent and the extract was chosen for further biological activities. The anti-bacterial assay revealed the inhibitory capacity, whereas, phyto-haemagglutination revealed clumping of blood cells due to parthenium extract exposure. The haemagglutination limit test revealed the minimum concentration of extract causes agglutination was 1/96 and the haemolytic activity result exhibited clear lysis of fish RBCs. LC50 of parthenium was found to be 18.99 mg l-1. The study revealed that significant (p < 0.05) reduction in haemato-immunological indices alongside serum biochemical parameters. The results presented in the study emphasise locally available weed plant gajar ghas, P. hysterophorus as a non-chemical phyto-ichthyotoxin.
The recovery of the microbial community after plaque removal depends on periodontal health status
Authors: Wang Jinfeng , Li Xiaoqing , Yu Cheng , Zhang Bing , Shan Xiaogang , Mao Wenjun , Zhang Zicheng , Wang Chunyan , Jin Xiaoxia , Zhao Hui 
Published: February 2023

Abstract
Plaque accumulation and microbial community changes are important causes of periodontal disease. Cleaned plaque microorganisms reattach to form biofilms, but the recovery and outcome of plaque microbial communities in different periodontal health states are still unknown. In this study, we tracked the biofilm remodeling process in 206 dental plaque samples from 40 healthy periodontal, gingivitis and periodontitis volunteers at 6 time points before and after supragingival scaling. We found that microbial communities of different periodontal states changed asynchronously during the process, and the more severe the periodontal disease condition, the more lagged the recovery of plaque microorganisms to their original state after cleaning; this reflected a higher degree of plaque development in periodontitis samples. The plaque index and bleeding index were found to be significantly correlated with plaque recovery, especially the recovery of bacteria such as Abiotrophia and Capnocytophaga. Meanwhile, we found that the microbial community structure of different periodontal health states was most similar at the Day 3 after plaque cleaning, and the communities gradually differentiated and developed in different directions. Abiotrophia and other bacteria might play an important role in determining the development trend of plaque biofilms. The discovery of specific time points and bacteria was of great value in clarifying the pathogenesis of periodontal disease and in seeking targets for prevention and treatment.
IOT Based Wrist Band for Women Safety
Authors: Ebenezer V. , Falicica Uvaana , Baskaran Rithika , Celesty Agatha , Eden Sejal R 
Published: February 2023

Abstract
In the modern world, women now have tremendous success in every field. They can play, learn, and earn as much as men. But what about safety? Do they have the same secure environment that men and boys do? The answer is ”NO”. Women and girls have been subjected to numerous incidents, including acid throwing, rape, kidnapping, and harassment. It is common to read a lot of news like this in newspapers every day. These incidents make women feel unsafe in this society. Our freedom came a long time ago, but women still lack complete security in this society. All women can’t fight or shout all the time when some danger is happening to them. What can the physically challenged person and Children do? To make women feel safe, we designed ”Wrist Band” using IoT for Women Safety. As the sensors sense information from the body, it will always update the information such as pulse, temperature and vibration to the well wishers through the blynk app.
Tuberculosis Detection Using Chest X-Ray Image Classification by Deep Learning
Authors: Guia Sana Sahar , Laouid Abdelkader , Kara Mostefa , Hammoudeh Mohammad 
Published: February 2023

Abstract
Tuberculosis is a serious and widespread lung disease, not all patients
are usually detected. After the availability of high-resolution
chest x-rays, deep learning can now provide solutions for the successful
detection of this malignant disease and other possible applications
in the health sector. In this manuscript, a new deep learning
model for Tuberculosis detection using chest x-ray image classification,
employing a mixture of two popular pre-trained vgg16 and
vgg19 utilizing the ImageNet dataset, and block attention module
in order to obtain spatial data. We validate the presented method
through experiments on four popular datasets. After evaluating the
model on the test set, we achieve a score of 0.9992 for all metrics
(accuracy, precision, recall, f1-score).
Genetic heterogeneity of cardiomyopathy and its correlation with patient care
Authors: Kim Mi Jin , Cha Seulgi , Baek Jae Suk , Yu Jeong Jin , Seo Go Hun , Kang Minji , Do Hyo-Sang , Lee Sang Eun , Lee Beom Hee 
Published: February 2023

Abstract
Background: Cardiomyopathy, which is a genetically and phenotypically heterogeneous pathological condition, is associated with increased morbidity and mortality. Genetic diagnosis of cardiomyopathy enables accurate phenotypic classification and optimum patient management and counseling. This study investigated the genetic spectrum of cardiomyopathy and its correlation with the clinical course of the disease.
Methods: The samples of 72 Korean patients with cardiomyopathy (43 males and 29 females) were subjected to whole-exome sequencing (WES). The familial information and clinical characteristics of the patients were reviewed and analyzed according to their genotypes.
Results: Dilated cardiomyopathy (DCM), hypertrophic cardiomyopathy (HCM), left ventricular non-compaction cardiomyopathy, and restrictive cardiomyopathy was detected in 41 (56.9%), 25 (34.7%), 4 (5.6%), and 2 (2.8%) patients, respectively. WES analysis revealed positive results in 29 (40.3%) patients. Subsequent familial testing identified nine additional familial cases. Among DCM cases, 15 (36.6%) patients exhibited positive results, with TTNvariations being the most common alteration, followed by LMNA and MYH7 variations. Meanwhile, among HCM cases, 13 (52%) patients exhibited positive results with MYH7 variations being the most common alteration. In six patients with positive results, extracardiac surveillance was warranted based on disease information. The incidence of worse outcomes, such as mortality and life-threatening arrhythmic events, in patients with DCM harboring LMNA variants, was higher than that in patients with DCM harboring TTN or MYH7 variants.
Conclusions: Diverse genotypes were identified in a substantial proportion of patients with cardiomyopathy. Genetic diagnosis enables personalized disease surveillance and management.
Temperature/Pressure/Humidity Coplanar Integrated SAW Sensor Based on LGS
Authors: Tan Qiulin , Liang Xiaorui , Zhang Lei , Cheng Wenhua , Hu Dan , Li Shuang , Xiong Jijun 
Published: February 2023

Abstract
In this paper, a three-parameter coplanar-integrated surface acoustic wave sensor based on Langasite is proposed. The micro-scale multi-parameter coplanar Temperature-Pressure-Humidity (TPH) sensor was developed by Micro-Electro-Mechanical System (MEMS) techniques such as wet etching, hot pressing bonding, and lifting off. The Mxene@ MoS2@Go (MMG) composite material was used as the humidity-sensitive material to enlarge the range of the humidity test and improve the sensitivity of the sensor test, and the humidity-sensitive unit shows high performance with a fast response time (3.18 s) and recovery time (0.94 s). The TPH sensor has been tested, and the experimental results indicate that the sensor can operate steadily at 25–700°C, 0-700 kPa, and 10–98% RH. Besides, a temperature decoupling approach is presented to address the issue of multi-parameter mutual coupling and improve the measurement accuracy of the sensitive unit while measuring several parameters at the same time. These excellent results are attributed to the coupling between many parameters in complex environments. Also, our work can be applied to the simultaneous measurement of several parameters in challenging conditions.
Variation in Pentose Phosphate Pathway-associated Metabolism Dictates Cytotoxicity Outcomes Determined by Tetrazolium Reduction Assays
Authors: Coyle Jayme P. , Johnson Caroline , Jensen Jake , Farcas Mariana , Derk Raymond , Stueckle Todd A. , Kornberg Tiffany G. , Rojanasakul Yon , Rojanasakul Liying 
Published: February 2023

Abstract
Tetrazolium reduction and resazurin assays are the mainstay of routine in vitro toxicity batteries. However, potentially erroneous characterization of cytotoxicity and cell proliferation can arise if verification of baseline interaction of test article with method employed is neglected. The current investigation aimed to demonstrate how interpretation of results from several standard cytotoxicity and proliferation assays vary in dependence on contributions from the pentose phosphate pathway (PPP). Non-tumorigenic Beas-2B cells were treated with graded concentrations of benzo[a]pyrene (B[a]P) for 24 and 48 hours prior to cytotoxicity and proliferation assessment with commonly used MTT, MTS, WST1, and Alamar Blue assays. B[a]P caused enhanced metabolism of each dye assessed despite reductions in mitochondrial membrane potential and was reversed by 6-aminonicotinamide (6AN) – a glucose-6-phosphate dehydrogenase inhibitor. These results demonstrate differential sensitivity of standard cytotoxicity assessments on the PPP, thus 1) decoupling “mitochondrial activity” as an interpretation of cellular formazan and Alamar Blue metabolism, and 2) demonstrating the implicit requirement for investigators to sufficiently verify interaction of these methods in routine cytotoxicity and proliferation characterization. The nuances of method-specific extramitochondrial metabolism must be scrutinized to properly qualify specific endpoints employed, particularly under the circumstances of metabolic reprogramming.
Impacts of educational debt on physical therapist employment trends
Authors: Dickson Tara , Hegedus Eric J , Mulligan Edward Patrick 
Published: February 2023

Abstract
Background: Newly graduated physical therapists have high amounts of educational debt.
Educational debt may negatively affect job satisfaction, aspirations for professional development, and choice of workplace setting. Research has not shown this association directly, yet it is conceptually supported by the Labor-Search Model. The purpose of this study was to understand the role that educational debt has on additional factors related to job choice in the Labor-Search Model.
Methods: Retrospective data were captured through the Virginia Longitudinal Data System (VLDS) for 12,594 licensed physical therapists within the Commonwealth of Virginia from 2014-2020. A fixed effects panel analysis, with inflation-adjusted educational debt as the variable of interest, was conducted to assess whether patterns of professional certifications, volume of work, workplace setting, and job satisfaction were related to educational debt.
Results: Educational debt was positively correlated with higher professional degrees (p = 0.009), the number of hours worked per week (p= 0.049), and expected number of years until retirement (p = 0.013). Job satisfaction was statistically significant (p = 0.042) and negatively correlated with educational debt.
Conclusions: Those with higher educational debt appear to have the habit of working more hours per week and have a longer time horizon until retirement. Newly licensed physical therapists with higher amounts of educational debt are more likely to experience this trend. Income and job satisfaction demonstrated an interaction effect on educational debt, such that those with lower levels of income had a stronger, negative relationship between their debt and job satisfaction, as compared to those with higher income.