bluuebunny/crossref_metadata_2025_split huggingface dataset:
LIFTOSCOPE: development of an automated AI-based module for time-effective and contactless analysis and isolation of cells in microtiter plates
Authors: Narrog Florian , Lensing Richard , Piotrowski Tobias , Nottrodt Nadine , Wehner Martin , Nießing Bastian , König Niels , Gillner Arnold , Schmitt Robert H. 
Published: February 2023

Abstract
Background
The cultivation, analysis, and isolation of single cells or cell cultures are fundamental to modern biological and medical processes. The novel LIFTOSCOPE technology aims to integrate analysis and isolation into one versatile, fully automated device.

Methods
LIFTOSCOPE’s three core technologies are high-speed microscopy for rapid full-surface imaging of cell culture vessels, AI-based semantic segmentation of microscope images for localization and evaluation of cells, and laser-induced forward transfer (LIFT) for contact-free isolation of cells and cell clusters. LIFT transfers cells from a standard microtiter plate (MTP) across an air gap to a receiver plate, from where they can be further cultivated. The LIFT laser is integrated into the optical path of an inverse microscope, allowing to switch quickly between microscopic observation and cell transfer.

Results
Tests of the individual process steps prove the feasibility of the concept. A prototype setup shows the compatibility of the microscope stage with the LIFT laser. A specifically designed MTP adapter to hold a receiver plate has been designed and successfully used for material transfers. A suitable AI algorithm has been found for cell selection.

Conclusion
LIFTOSCOPE speeds up cell cultivation and analysis with a target process time of 10 minutes, which can be achieved if the cell transfer is sped up using a more efficient path-finding algorithm. Some challenges remain, like finding a suitable cell transfer medium.

Significance
The LIFTOSCOPE system can be used to extend existing cell cultivation systems and microscopes for fully automated biotechnological applications.
Comparative transcriptome and co-expression network analysis revealed the genes associated with senescence and polygalacturonase activity involved in pod shattering of rapeseed
Authors: Mahmood Umer , Li Xiaodong , Qian Mingchao , Fan Yonghai , Yu Mengna , Li Shengting , Shahzad Ali , Qu Cunmin , Li Jiana , Liu Liezhao , Lu Kun 
Published: February 2023

AbstractBackgroundThe pod shattering (PS) trait negatively affects the crop yield in rapeseed especially under dry conditions. To better understand the trait and cultivate higher resistance varieties, it’s necessary to identify key genes and unravel the PS mechanism thoroughly.ResultsIn this study, we conducted a comparative transcriptome analysis between two materials significantly different in silique shatter resistance lignin deposition and polygalacturonase (PG) activity. Here, we identified 10,973 differentially expressed genes at six pod developmental stages. We found that the late pod development stages might be crucial in preparing the pods for upcoming shattering events. GO enrichment results fromK-means clustering and weighed gene correlation network analysis (WGCNA) both revealed senescence-associated genes play an important role in PS. Two hub genesBna.A05ABI5andBna.C03ERF/AP2-3were selected from the MEyellow module, which possibly regulate the PS through senescence-related mechanisms. Further investigation found that senescence-associated transcription factorBna.A05ABI5upregulated the expression ofSAG2andERF/AP2to control the shattering process. In addition, the upregulation ofBna.C03ERF/AP2-3is possibly involved in the transcription of downstreamSHP1/2and LEA proteins to trigger the shattering mechanism. We also analyzed the PS marker genes and foundBna.C07SHP1/2 andBna.PG1/2were significantly upregulated in susceptible accession. Furthermore, the role of auxin transport byBna.WAG2was also observed, which could reduce the PG activity to enhance the PS resistance through the cell wall loosening process.ConclusionBased on comparative transcriptome evaluation, this study delivers insights into the regulatory mechanism primarily underlying the variation of PS in rapeseed. Taken together, these results provide a better understanding to increase the yield of rapeseed by reducing the PS through better engineered crops.
Review of methods for coding of speech signals
Authors: O’Shaughnessy Douglas 
Published: February 2023

AbstractSpeech is the most common form of human communication, and many conversations use digital communication links. For efficient transmission, acoustic speech waveforms are usually converted to digital form, with reduced bit rates, while maintaining decoded speech quality. This paper reviews the history of speech coding techniques, from early mu-law logarithmic compression to recent neural-network methods. The techniques are examined in terms of output quality, algorithmic complexity, delay, and cost. Focus is on which aspects of speech can be exploited for high-quality transmission. The choices made to code speech are motivated by efficiency, the needs of applications, and access to information in the speech signal that is useful for both intelligibility and naturalness in the reconstructed speech at the decoder.
Local limit theorems without assuming finite third moment
Authors: Kammoo Punyapat , Laipaporn Kittipong , Neammanee Kritsana 
Published: February 2023

AbstractOne of the most fundamental probabilities is the probability at a particular point. The local limit theorem is the well-known theorem that estimates this probability. In this paper, we estimate this probability by the density function of normal distribution in the case of lattice integer-valued random variables. Our technique is the characteristic function method. We complete to relax the third moment condition of Siripraparat and Neammanee (J. Inequal. Appl. 2021:57, 2021) and the references therein and also obtain explicit constants of the error bound.
Grayken lessons: the role of an interdisciplinary endocarditis working group in evaluating and optimizing care for a woman with opioid use disorder requiring a second tricuspid valve replacement
Authors: Rozansky Hallie , Awtry Eric , Weinstein Zoe M. , Peterkin Alyssa F. 
Published: February 2023

Abstract
Background
Injection drug use-related endocarditis is increasingly common among hospitalized patients in the United States, and associated morbidity and mortality are rising.

Case presentation
Here we present the case of a 34-year-old woman with severe opioid use disorder and multiple episodes of infective endocarditis requiring prosthetic tricuspid valve replacement, who developed worsening dyspnea on exertion. Her echocardiogram demonstrated severe tricuspid regurgitation with a flail prosthetic valve leaflet, without concurrent endocarditis, necessitating a repeat valve replacement. Her care was overseen by our institution’s Endocarditis Working Group, a multidisciplinary team that includes providers from addiction medicine, cardiology, infectious disease, cardiothoracic surgery, and neurocritical care. The team worked together to evaluate her, develop a treatment plan for her substance use disorder in tandem with her other medical conditions, and advocate for her candidacy for valve replacement.

Conclusions
Multidisciplinary endocarditis teams such as these are important emerging innovations, which have demonstrated improvements in outcomes for patients with infective endocarditis and substance use disorders, and have the potential to reduce bias by promoting standard-of-care treatment.
The role and regulation of Maf proteins in cancer
Authors: Deng Yalan , Lu Liqing , Zhang Huajun , Fu Ying , Liu Ting , Chen Yongheng 
Published: February 2023

AbstractThe Maf proteins (Mafs) belong to basic leucine zipper transcription factors and are members of the activator protein-1 (AP-1) superfamily. There are two subgroups of Mafs: large Mafs and small Mafs, which are involved in a wide range of biological processes, such as the cell cycle, proliferation, oxidative stress, and inflammation. Therefore, dysregulation of Mafs can affect cell fate and is closely associated with diverse diseases. Accumulating evidence has established both large and small Mafs as mediators of tumor development. In this review, we first briefly describe the structure and physiological functions of Mafs. Then we summarize the upstream regulatory mechanisms that control the expression and activity of Mafs. Furthermore, we discuss recent studies on the critical role of Mafs in cancer progression, including cancer proliferation, apoptosis, metastasis, tumor/stroma interaction and angiogenesis. We also review the clinical implications of Mafs, namely their potential possibilities and limitations as biomarkers and therapeutic targets in cancer.
Inverse correlation of free triiodothyronine with glycated albumin and the glycated albumin/glycated hemoglobin ratio in hemodialysis patients: a cross-sectional study
Authors: Mimura Keiji , Nishide Takahiro , Naganuma Toshihide , Katsube Yui , Ishitani Seiji , Nakahara Katsuhiko , Hirasaka Naohisa , Mizobata Rie , Yamagata Yuko , Nishikawa Osamu , Yukawa Susumu 
Published: February 2023

Abstract
Background
That the prevalence of low triiodothyronine (T3) syndrome is high among hemodialysis (HD) patients has been previously established. Herein, we examined the association of glycated albumin (GA) and the GA to glycated hemoglobin (HbA1c) ratio (GA/HbA1c) with free triiodothyronine (FT3) in HD patients.

Methods
We conducted a cross-sectional study on 134 patients (68 patients with diabetes mellitus [DM group] and 66 patients without diabetes mellitus [non-DM group]) who received maintenance HD at our dialysis clinic between 2014 and 2018. Univariate linear regression analyses of GA, GA/HbA1c, or HbA1c with several clinical variables were primarily conducted. Multiple regression analyses with GA (or GA/HbA1c) as the objective variable were conducted with explanatory variable FT3 adjusted for age, sex, Hb, Alb, and average plasma glucose (Av-PG) (or HbA1c).

Results
In the DM and non-DM groups, GA tended to be inversely correlated with FT3, although significantly so only in the non-DM group. GA/HbA1c also showed a strong significant inverse correlation with FT3 in the DM group and the non-DM group. FT3 and GA/HbA1c were also significantly correlated with the Geriatric Nutritional Risk Index in the DM group and non-DM group. In the multivariate analysis, which was adjusted for age, sex, Hb, Alb, and HbA1c, FT3 was a significant and independent factor associated with GA in the DM group (β = − 0.334, p < 0.001) and in the non-DM group (β = − 0.412, p < 0.001). The regression equations obtained by stepwise multiple regression analyses using all of these variables as independent variables were GA = 3.3HbA1c − 4.4FT3 + 1.9sex + 8.8 for the DM group and GA =  − 2.4FT3 + 0.04Age − 0.5Hb + 25.2 for the non-DM group. These contribution rates (i.e., coefficient of determination) were R2 = 0.708 in the DM group and R2 = 0.347 in the non-DM group, In the DM group, the estimation formulas, based on the regression equation [GA (men) = 3.3HbA1c − 4.4FT3 + 10.7 and GA (women) = 3.3HbA1c − 4.4FT3 + 8.8], showed very high contribution rates (i.e., coefficient of determination R2 = 0.674 for men and 0.761 for women) for the GA measured values.

Conclusions
GA and GA/HbA1c have a close relationship with FT3 in HD patients. The estimation formulas of GA could be obtained. In particular, the estimation formulas in the DM group are believed to be useful in considering HbA1c and FT3 simultaneously when evaluating GA.
Measuring the effect of employment uncertainty on fertility in low-fertility contexts: an overview of existing measures
Authors: Buh Brian 
Published: February 2023

AbstractNumerous studies aim to connect negative fertility desires and outcomes with employment conditions deemed to be uncertain. However, there is a lack of consensus about how to define, conceptualise, and measure employment uncertainty. This paper considers issues surrounding the conceptualisation of employment uncertainty. It then reviews existing measures of employment uncertainty in the context of fertility decisions. Finally, it raises considerations about their use. While some aspects of employment uncertainty are well studied, there are still gaps between theory and empirical evidence. Researchers should be aware of existing population heterogeneity, contextual factors, and model selection when considering their conceptualisation of employment uncertainty.
Development and internal validation of a diagnostic prediction model for psoriasis severity
Authors: Liljendahl Mie Sylow , Loft Nikolai , Egeberg Alexander , Skov Lone , Nguyen Tri-Long 
Published: February 2023

Abstract
Background
While administrative health records such as national registries may be useful data sources to study the epidemiology of psoriasis, they do not generally contain information on disease severity.

Objectives
To develop a diagnostic model to distinguish psoriasis severity based on administrative register data.

Method
We conducted a retrospective registry-based cohort study using the Danish Skin Cohort linked with the Danish national registries. We developed a diagnostic model using a gradient boosting machine learning technique to predict moderate-to-severe psoriasis. We performed an internal validation of the model by bootstrapping to account for any optimism.

Results
Among 4016 adult psoriasis patients (55.8% women, mean age 59 years) included in this study, 1212 (30.2%) patients were identified as having moderate-to-severe psoriasis. The diagnostic prediction model yielded a bootstrap-corrected discrimination performance: c-statistic equal to 0.73 [95% CI: 0.71–0.74]. The internal validation by bootstrap correction showed no substantial optimism in the results with a c-statistic of 0.72 [95% CI: 0.70–0.74]. A bootstrap-corrected slope of 1.10 [95% CI: 1.07–1.13] indicated a slight under-fitting.

Conclusion
Based on register data, we developed a gradient boosting diagnostic model returning acceptable prediction of patients with moderate-to-severe psoriasis.
Intraspecific body size variability in a population of Billaea rhynchophorae (Blanchard) (Diptera: Tachinidae) parasitizing Rhynchophorus palmarum (Linnaeus) (Coleoptera: Curculionidae) in Brazil
Authors: Guzzo Elio Cesar , Toma Ronaldo , Acevedo Juan Pablo Molina , Vega Jackeline Gaviria , Negrisoli Aldomario Santo , Löhr Bernhard Leo , dos Santos Tamara Tais , da Silva Diego Jorge , Diniz Leandro Eugênio Cardamone 
Published: February 2023

AbstractBackgroundThe black palm weevil,Rhynchophorus palmarum(Linnaeus, 1758) (Coleoptera: Curculionidae) is considered the most important palm pest in the Americas. In the State of Bahia, Brazil, it is parasitized by tachinid flies (Diptera: Tachinidae). During 2019–2020, larvae and pupae ofR. palmarumwere collected from African oil palms (Elaeis guineensisJacq.) in the municipalities of Jaguaripe and Taperoá, Bahia State, Brazil. Puparia and adult tachinids of two apparent morphospecies, with considerable differences in body size, were obtained from theR. palmarumimmatures, which led to initially assume that we had collected two different tachinid species.ResultsMorphological identification as well asCOIgene sequencing showed that the two apparent morphospecies belong to a single taxonomic species,Billaea rhynchophorae(Blanchard, 1937) (Diptera: Tachinidae).ConclusionsOccurrence of intraspecific size variability inB. rhynchophoraewas reported, as well as performed the first genetic sequencing for this species. The reason of the size variation could not be determined but some possible causes and implications are discussed.
The double-balloon technique: a safe and effective adjunctive technique in patients undergoing arterial therapy for hepatic malignancies with vascular supply not amenable to selective administration
Authors: Tanaka Mari , Uppot Raul , Daye Dania , Liu Raymond , Wehrenberg-Klee Eric 
Published: February 2023

Abstract
Purpose
During catheter directed intraarterial therapy for liver lesions, challenging hepatic vascular anatomy can sometimes prevent selective administration of treatment delivery to liver tumors leading to increased toxicity to normal liver parenchyma. The objective of this study is to describe a variation of the double balloon technique that isolates the feeding artery to liver tumors proximally and distally to provide treatment delivery in lesions that cannot be otherwise selected.

Materials and methods
An IRB-approved retrospective review of 7 patients who had undergone either radioembolization, chemoembolization, or bland embolization and the double balloon technique was employed. The devices used for flow augmentation were two 2.1 French balloon microcatheters (Sniper™, Embolx). One balloon was inflated distal to target vessel and the second was inflated proximal to protect from reflux.

Results
DEB-TACE was performed in 3 cases, 90Y was performed in 4, and bland embolization was performed in the last patient. There were no adverse effects from the procedure or clinically evident effects from non-target embolization. Mean follow up time was 286.4 +/− 200.1 days. Six of the 7 patients are alive. One patient passed away on post-procedure day 121 from septic shock unrelated to the procedure. One patient was bridged to transplant with an additional TACE of a separate lesion.

Conclusion
Double-balloon technique for patients undergoing 90Y or chemoembolization is a safe adjunctive technique for super selective treatment of hepatic lesions where direct selection via catheter is not feasible. This may increase the range of lesions that can be both safely and effectively treated by catheter directed therapies.
Structured illumination microscopy based on principal component analysis
Authors: Qian Jiaming , Cao Yu , Bi Ying , Wu Hongjun , Liu Yongtao , Chen Qian , Zuo Chao 
Published: February 2023

AbstractStructured illumination microscopy (SIM) is one of the powerful super-resolution modalities in bioscience with the advantages of full-field imaging and high photon efficiency. However, artifact-free super-resolution image reconstruction requires precise knowledge about the illumination parameters. The sample- and environment-dependent on-the-fly experimental parameters need to be retrieved a posteriori from the acquired data, posing a major challenge for real-time, long-term live-cell imaging, where low photobleaching, phototoxicity, and light dose are a must. In this work, we present an efficient and robust SIM algorithm based on principal component analysis (PCA-SIM). PCA-SIM is based on the observation that the ideal phasor matrix of a SIM pattern is of rank one, leading to the low complexity, precise identification of noninteger pixel wave vector and pattern phase while rejecting components that are unrelated to the parameter estimation. We demonstrate that PCA-SIM achieves non-iteratively fast, accurate (below 0.01-pixel wave vector and 0.1$$\%$$
%
of 2$$\pi$$
π
relative phase under typical noise level), and robust parameter estimation at low SNRs, which allows real-time super-resolution imaging of live cells in complicated experimental scenarios where other state-of-the-art methods inevitably fail. In particular, we provide the open-source MATLAB toolbox of our PCA-SIM algorithm and associated datasets. The combination of iteration-free reconstruction, robustness to noise, and limited computational complexity makes PCA-SIM a promising method for high-speed, long-term, artifact-free super-resolution imaging of live cells.
The Potential Impact of Basivertebral Foramen Morphology and Pedicle Screw Placement on Epidural Cement Leakage With Cement-Augmented Fenestrated Pedicle Screw Fixation: A Multicenter Retrospective Study of 282 Patients and 1404 Augmented Screws
Authors: Yu Weibo , Liang De , Yao Zhensong , Zhang Haiyan , Zhong Yuanming , Tang Yongchao , Jiang Xiaobing , Cai Daozhang 
Published: February 2023

BACKGROUND:
Epidural cement leakage (CL) is a common complication in cement-augmented fenestrated pedicle screw fixation (CAFPSF) with the potential for neural injury. However, there are no reports discussing basivertebral vein morphology and pedicle screw placement, which are critical in the analysis of the risk of epidural CL after CAFPSF.

OBJECTIVE:
To identify the incidence and risk factors of epidural CL in osteoporotic patients during CAFPSF.

METHODS:
Two hundred and eighty-two osteoporotic patients using 1404 cement-augmented fenestrated screws were included. Preoperative computed tomography (CT) was used to characterize the morphology of posterior cortical basivertebral foramen. After CAFPSF, the severity of epidural CL, the implantation position of the screw tip, and cement extension within the vertebral body were determined by postoperative CT scans. In this study, significant risk factors for epidural CL were identified through logistic regression analysis.

RESULTS:
In total, 28 patients (18.8%) and 108 screws (7.7%) had epidural CL and 7 patients (13 screws) experienced neurological symptoms. Although local epidural CL was generally not clinically significant, extensive epidural leakage posed a higher risk of neurological symptoms. Significant predictors for extensive epidural CL were a magistral type of basivertebral foramen and the smaller distance between the tip of the screw and the posterior wall of the vertebral body.

CONCLUSION:
In osteoporotic patients receiving CAFPSF, epidural CL is relatively common. The morphology of basivertebral foramen should be taken into account when planning a CAFPSF procedure. It is important to try and achieve a deeper screw implantation, especially when a magistral type of basivertebral foramen is present.