bluuebunny/crossref_metadata_2025_split huggingface dataset:
Cross-Cultural Differences in Stigma Associated with Parkinson’s Disease: A Systematic Review
Authors: Karacan Ahmet Veli , Kibrit Sema Nur , Yekedüz Merve Koç , Doğulu Neslihan , Kayis Gorkem , Unutmaz Elif Yüsra , Abali Talha , Eminoğlu F. Tuba , Akbostancı M. Cenk , Yilmaz Rezzak 
Published: June 2023

Background: Stigma is an important social attitude affecting the quality of life (QoL) of people with Parkinson’s disease (PwP, PD) as individuals within society. Objective: This systematic review aimed to 1) identify the factors associated with stigma in PD and 2) demonstrate culture-based diversity in the stigmatization of PwP. We also reported data from the Turkish PwP, which is an underrepresented population. Methods: Following the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) statement, a literature search of the PubMed/Medline electronic database was performed covering the last 26 years. Articles on self-perceived stigma in PD with a sample size >  20 and quantitative results were included. Data were extracted by independent reviewers. Results: After screening 163 articles, 57 were eligible for review, most of which were from Europe or Asia. Only two studies have been conducted in South America. No study from Africa was found. Among the 61 factors associated with stigma, disease duration, sex, and age were most frequently studied. A comparison of the investigated factors across the world showed that, while the effect of motor impairment or treatment on stigma seems to be culture-free, the impact of sex, education, marriage, employment, cognitive impairment, and anxiety on stigma may depend on culture. Conclusion: The majority of the world’s PD population is underrepresented or unrepresented, and culture may influence the perception of stigma in PwP. More diverse data are urgently needed to understand and relieve the challenges of PwP within their society.
Efficiency of using larch bark extract as a feed additive for calves
Authors: Aminova A. L. , Yumaguzin I. F. , Sharifyanov B. G. , Kolesnik A. B. 
Published: June 2023

Relevance. In recent years, the search and synthesis of new more effective plant extracts have been intensively conducted, which also require extensive scientific and industrial tests. These include the search for local resources and the simplest methods of biotechnology and harvesting plant extracts, including feed additives. In this regard, the study of the use of a plant extract based on larch bark is of great importance and is an urgent area of research. The aim of the work is to determine the effectiveness of the use of larch bark extract as a feed additive to increase the natural resistance and intensity of growth of live weight of cattle calves.Methods. For the study, we used: larch bark extract and raido remedy in a dosage of 5 g per calfʼs head for 10 days. Hematological studies were carried out on a Beckman Coulter LH-500 analyzer using the Culture method. The activity of blood serum was determined by the methods of V.G. Dorofeichuk, O.V. Smirnova and G.A. Kuzmina.Results. Studies have found that in order to stimulate the intensity of growth of live weight of cattle in the early dairy period of cultivation, it is recommended to use a plant extract from the bark of larch, which allows to increase the intensity of growth and get an additional increase in live weight by 9.0% compared with calves of the control group. The use of the extract stimulates the formation of nonspecific resistance, which is manifested in an increase in phagocytic (by 8.5%), bactericidal (by 8.9%), lysozyme (by 6.6%) activity of the blood serum of calves compared with the control group
Faktor-faktor yang Mempengaruhi Kecurangan Akuntansi (Studi Kasus pada PT. Bank Tabungan Negara (Persero) Tbk KC Medan)
Authors: Siregar Ahmad Aidil , Hanafi Lubis Reza 
Published: June 2023

Penelitian ini dilakukan di PT. Bank Tabungan Negara (Persero) Tbk KC Medan yang betujuan untuk menguji dan menganalisis faktor-faktor yang mempengaruhi kecurangan akuntansi yang terdiri atas pengendalian internal, moralitas individu, motivasi dan ketaatan aturan akuntansi. Penelitian ini menggunakan metode kuantitatif dengan instrumen kuesioner. Populasi dalam penelitian ini adalah seluruh pegawai yang ada di PT. Bank Tabungan Negara (Persero) Tbk KC Medan yang berjumlah 85 orang. Jumlah sampel yang dipilih dengan metode purposive sampling ialah sebanyak 37 orang. Metode analisis yang digunakan yaitu analisis regresi linear berganda. Hasil penelitian menunjukkan bahwa pengendalian internal, moralitas individu dan motivasi secara parsial memiliki pengaruh positif dan signifikan terhadap kecurangan akuntansi, serta ketaatan aturan akuntansi secara parsial memiliki pengaruh negatif dan signifikan terhadap kecurangan akuntansi di PT. Bank Tabungan Negara (Persero) Tbk KC Medan. Sedangkan secara simultan pengendalian internal, moralitas individu, motivasi dan ketaatan aturan akuntansi memiliki pengaruh positif dan signifikan terhadap kecurangan akuntansi di PT. Bank Tabungan Negara (Persero) Tbk KC Medan.
The flow of scientific books as a source for scientific library collection development: The problems of completeness and accessibility
Authors: Podkorytova N. I. , Kuular M. Ch. , Makhotina N. V. 
Published: June 2023

Purpose.The authors analyze the trends in the national flow of book publications in science for the period 2012–2021 and its impact upon scientific library collections.Sources and methods. The study is based on the statistical data of large information systems, i.e. the Russian Book Chamber and scientific electronic library eLibrary.ru), and State Public Scientific and Technological Library of the Russian Academy Siberian Branch (RAS SB SPSTL). The data of the Higher School of Economics characterize scientific focuses of Russian researchers.Findings and conclusions. The data on the flow of scientific book publications were analyzed to confirm that after 2014, the volume of science publications has been continuously decreasing. It is especially true for academic book publishing. The trend for a disproportion in the discipline structure of scientific book flow, and for scattering information on scientific book publications, is revealed. It is due to the lack of coordination between scientific content creators and institutions intended to acquire complete data on scientific document flow. Science information support and knowledge generation require special infrastructure necessarily embracing scientific and academic libraries.
Evaluation of the red seaweed Mazzaella japonica as a feed additive for beef cattle
Authors: Terry Stephanie A. , Coates Trevor , Gruninger Robert , Abbott D. Wade , Beauchemin Karen A. 
Published: June 2023

Supplementing ruminant diets with macroalgae is gaining interest globally because bromoform-containing seaweeds (e.g., Asparagopsis spp.) have been shown to be highly effective enteric methane (CH4) inhibitors. Some alternative seaweeds decrease in vitro CH4 production, but few have been evaluated in animals. This study examined the effects of including the red seaweed Mazzaella japonica in the diet of beef cattle on dry matter intake (DMI), rumen fermentation, digestibility, nitrogen (N) utilization, and enteric CH4 production. Six ruminally cannulated, mature beef heifers (824 ± 47.1 kg) were used in a double 3 × 3 Latin square with 35-d periods. The basal diet consisted of 52% barley silage, 44% barley straw, and 4% vitamin and mineral supplement [dry matter (DM) basis]. The treatments were (DM basis): 0% (control), 1%, and 2% M. japonica. The DMI increased quadratically (P = 0.025) with the inclusion of M. japonica, such that the DMI of heifers consuming 1% was greater (P &lt; 0.05) than that of control heifers. The apparent total-tract digestibility of DM decreased linearly (P = 0.002) with the inclusion of M. japonica, but there were no treatment differences in the digestibility of organic matter, crude protein (CP), neutral detergent fiber, or starch. The level of M. japonica linearly (P &lt; 0.001) increased the N intake of the heifers. Fecal N excretion linearly increased (P = 0.020) with M. japonica, but there were no differences in total urinary N excretion, N fractions (allantoin, uric acid), total purine derivatives, microbial purine derivatives absorbed, microbial N flow, or retained N. There were no treatment effects on rumen pH or total volatile fatty acids (VFAs); however, adding M. japonica to the diet quadratically (P = 0.023) decreased the proportion of acetate, whereas 1% inclusion decreased the acetate proportion. Methane production (g/day) decreased quadratically (P = 0.037), such that the heifers receiving 2% M. japonica produced 9.2% less CH4 than control animals; however, CH4 yield (g/kg DMI) did not differ among treatments. We conclude that supplementing a forage-based diet with up to 2% M. japonica failed to lower the enteric CH4 yield of beef heifers. M. japonica can be used in diets to help meet the CP requirements of cattle, but inclusion rates may be limited by high inorganic matter proportions. When comprising up to 2% of the diet, M. japonica cannot be recommended as a CH4 inhibitor for beef cattle fed on high-forage diets.
Zinc supplementation and ractopamine hydrochloride impact gene expression of zinc transporters in finishing beef steers
Authors: Rients Emma L. , Wyatt Remy N. , Deters Erin L. , Genther-Schroeder Olivia N. , Hansen Stephanie L. 
Published: June 2023

Zinc is a trace mineral of interest for optimizing growth in feedlot cattle due to its roles in many physiological functions, including growth. Twenty-four Angus-cross steers (467 ± 13 kg) were used to assess the effects of supplemental Zn and ractopamine hydrochloride (RAC) on trace mineral concentrations and muscle gene expression. Four GrowSafe-equipped pens were randomly assigned to treatments (1 pen of six steers/treatment): 0 (CON), 60 (LOW), 120 (MED) or 180 (HI) mg supplemental Zn/kg DM (Availa-Zn, Zinpro). Dietary Zn treatments were initiated on d 0 and RAC supplementation (300 mg·steer·-1·d-1; Actogain45, Zoetis) began on d 53. Blood, liver and muscle (longissimus thoracis) samples were collected from all steers on d -4, 48, and 67. The LOW treatment was removed from gene expression analyses due to &lt; 3 steers being represented for 14 of 22 genes. Data were analyzed using ProcMixed of SAS with the fixed effect of treatment and steer as the experimental unit; orthogonal linear and quadratic contrast statements were used to compare treatments. On d 48 and 67, there were linear and quadratic trends for plasma Zn to be greater in Zn-supplemented steers than CON (P ≤ 0.10). On d 48, there was a tendency for a quadratic decrease on the expression of SLC30A4 (P ≤ 0.07) but no other differences due to treatment. On d 67, several genes involved in Zn transport and storage (MTA1, SLC39A7, SLC39A8, SLC39A9, SLC39A10, SLC39A13) were decreased (P ≤ 0.08), suggesting increased growth influences intracellular Zn trafficking and demands.
Preparation of photothermal responsive, antibacterial hydrogel by using PVA-Alg and silver nanofibers as building blocks
Authors: Ding Jiaxin , Gao Binbin , Mei Xifan 
Published: June 2023

Introduction: Photothermal responsive, antimicrobial hydrogels are very attractive and have great potential in the field of tissue engineering. The defective wound environment and metabolic abnormalities in diabetic skin would lead to bacterial infections. Therefore, multifunctional composites with antimicrobial properties are urgently needed to improve the current therapeutic outcomes of diabetic wounds. We prepared an injectable hydrogel loaded with silver nanofibers for efficient and sustained bactericidal activity.Methods: To construct this hydrogel with good antimicrobial activity, homogeneous silver nanofibers were first prepared by solvothermal method and then dispersed in PVA-lg solution. After homogeneous mixing and gelation, injectable hydrogels (Ag@H) wrapped with silver nanofibers were obtained.Results: By virtue of Ag nanofibers, Ag@H exhibited good photothermal conversion efficiency and good antibacterial activity against drug-resistant bacteria, while the in vivo antibacterial also showed excellent performance. The results of antibacterial experiments showed that Ag@H had significant bactericidal effects on MRSA and E. coli with 88.4% and 90.3% inhibition rates, respectively.Discussion: The above results indicate that Ag@H with photothermal reactivity and antibacterial activity is very promising for biomedical applications, such as wound healing and tissue engineering.
Chemical profiling of Sanjin tablets and exploration of their effective substances and mechanism in the treatment of urinary tract infections
Authors: Li Meng-Yuan , Li Yang , Wang Li-Li , Xu Feng , Guo Xu-Yan , Zhang Jing , Lv Yang , Wang Peng-Pu , Wang Shun-Qi , Min Jian-Guo , Zou Xun , Cai Shao-Qing 
Published: June 2023

Introduction: Sanjin tablets (SJT) are a well-known Chinese patent drug that have been used to treat urinary tract infections (UTIs) for the last 40 years. The drug consists of five herbs, but only 32 compounds have been identified, which hinders the clarification of its effective substances and mechanism.Methods: The chemical constituents of SJT and their effective substances and functional mechanism involved in the treatment of UTIs were investigated by using high performance liquid chromatography-electrospray ionization-ion trap-time of flight-mass spectrometry (HPLC-ESI-IT-TOF-MSn), network pharmacology, and molecular docking.Results: A total of 196 compounds of SJT (SJT-MS) were identified, and 44 of them were unequivocally identified by comparison with the reference compounds. Among 196 compounds, 13 were potential new compounds and 183 were known compounds. Among the 183 known compounds, 169 were newly discovered constituents of SJT, and 93 compounds were not reported in the five constituent herbs. Through the network pharmacology method, 119 targets related to UTIs of 183 known compounds were predicted, and 20 core targets were screened out. Based on the “compound–target” relationship analysis, 94 compounds were found to act on the 20 core targets and were therefore regarded as potential effective compounds. According to the literature, 27 of the 183 known compounds were found to possess antimicrobial and anti-inflammatory activities and were verified as effective substances, of which 20 were first discovered in SJT. Twelve of the 27 effective substances overlapped with the 94 potential effective compounds and were determined as key effective substances of SJT. The molecular docking results showed that the 12 key effective substances and 10 selected targets of the core targets have good affinity for each other.Discussion: These results provide a solid foundation for understanding the effective substances and mechanism of SJT.
Antioxidant activity of lidocaine, bupivacaine, and ropivacaine in aqueous and lipophilic environments: an experimental and computational study
Authors: Kavčič H. , Jug U. , Mavri J. , Umek N. 
Published: June 2023

Introduction: Local anesthetics are widely recognized pharmaceutical compounds with various clinical effects. Recent research indicates that they positively impact the antioxidant system and they may function as free radical scavengers. We hypothesize that their scavenging activity is influenced by the lipophilicity of the environment.Methods: We assessed the free radical scavenging capacity of three local anesthetics (lidocaine, bupivacaine, and ropivacaine) using ABTS, DPPH, and FRAP antioxidant assays. We also employed quantum chemistry methods to find the most probable reaction mechanism. The experiments were conducted in an aqueous environment simulating extracellular fluid or cytosol, and in a lipophilic environment (n-octanol) simulating cellular membranes or myelin sheets.Results: All local anesthetics demonstrated ABTS˙+ radical scavenging activity, with lidocaine being the most effective. Compared to Vitamin C, lidocaine exhibited a 200-fold higher half-maximal inhibitory concentration. The most thermodynamically favorable and only possible reaction mechanism involved hydrogen atom transfer between the free radical and the -C-H vicinal to the carbonyl group. We found that the antioxidant activity of all tested local anesthetics was negligible in lipophilic environments, which was further confirmed by quantum chemical calculations.Conclusion: Local anesthetics exhibit modest free radical scavenging activity in aqueous environments, with lidocaine demonstrating the highest activity. However, their antioxidant activity in lipophilic environments, such as cellular membranes, myelin sheets, and adipose tissue, appears to be negligible. Our results thus show that free radical scavenging activity is influenced by the lipophilicity of the environment.
Human and porcine aortic valve endothelial and interstitial cell isolation and characterization
Authors: Nehl D. , Goody P. R. , Maus K. , Pfeifer A. , Aikawa E. , Bakthiary F. , Zimmer S. , Nickenig G. , Jansen F. , Hosen M. R. 
Published: June 2023

BackgroundCalcific aortic valve stenosis (AVS) is defined by pathological changes in the aortic valve (AV) and their predominant cell types: valvular interstitial (VICs) and endothelial cells (VECs). Understanding the cellular and molecular mechanisms of this disease is a prerequisite to identify potential pharmacological treatment strategies. In this study, we present a unique aortic valve cell isolation technique to acquire specific human and porcine cell populations and compared VICs and VECs of these species with each other for the first time.MethodsAV cells were isolated from tissue obtained from human patients undergoing surgical aortic valve replacement (SAVR) or from porcine hearts. Functional analysis and in vitro experiments revealed that endothelial-to-mesenchymal transition (EndMT) can be induced in hVECs, leading to a significant increase in mesenchymal markers. In vitro calcification experiments of VICs demonstrated pronounced expression of calcification markers and visible calcific deposits in Alizarin Red staining in both species after incubation with pro-calcific media.ResultsCells isolated from patient-derived AVs showed mesenchymal and endothelial-specific gene signatures (VIC and VEC, respectively). For instance, von Willebrand factor (vWF) and platelet endothelial adhesion molecule-1 (PECAM1) were upregulated in VECs, while the myofibroblastic markers alpha-smooth muscle actin (α-SMA) and vimentin (VIM) were downregulated in VECs compared to VICs. Analysis of cell function by migration revealed that VECs are more migratory than VICs. Induction of EndMT in vitro in VECs displayed increased expression of EndMT markers and decreased expression of endothelial markers, confirming their mesenchymal transdifferentiation ability. In vitro calcification of VICs revealed upregulation of alkaline phosphatase (ALPL), a hallmark of calcification. In addition, other calcification-related genes such as osteocalcin (BGLAP) and runt-related factor 2 (RUNX2) were upregulated. Alizarin red staining of calcified cells provided a further layer of confirmation that the isolated cells were VICs with osteoblastic differentiation capacity.ConclusionThis study aims to take a first step towards standardizing a reproducible isolation technique for specific human and porcine VEC and VIC populations. A comparison of human and porcine aortic valve cells demonstrated that porcine cells may serve as an alternative cellular model system in settings where human tissue is difficult to obtain.
Is blue light exposure a cause of precocious puberty in male rats?
Authors: Uğurlu Aylin Kılınç , Bideci Aysun , Demirel Ayşe Mürşide , Kaplanoğlu Gülnur Take , Dayanır Duygu , Gülbahar Özlem , Bulut Tuba Saadet Deveci , Döğer Esra , Çamurdan Mahmut Orhun 
Published: June 2023

PurposeOur study aimed to examine the effects of blue light exposure on prepubertal male rats’ puberty and testis tissue.MethodsEighteen 21-day-old male Sprague Dawley rats were divided into three groups consisting of six rats in each group: Control Group (CG), Blue Light-6 hours (BL-6), and Blue Light-12 hours (BL-12). CG rats were maintained with 12/12-hour light-dark cycles. The rats of BL-6 and BL-12 were exposed to blue light (450-470nm/irradiance level 0.03uW/cm2) for 6 hours and 12 hours, respectively. Rats were exposed to blue light until the first signs of puberty. The ELISA method was used to analyze the serum levels of FSH, LH, testosterone, DHEA-S, leptin, ghrelin, melatonin, glutathione, glutathione peroxidase, and malondialdehyde. Testes were dissected for histomorphological examination.ResultsThe medians of the pubertal entry days of the CG, BL-6, and BL-12 were 38th, 30th, and 28th days, respectively. (p:0.001) The FSH, LH, and testosterone concentrations of all groups were similar. The FSH concentration increased as the LH concentration increased (r: 0.82 p: 0.001). The serum LH concentration increased as serum testosterone, and DHEAS decreased, respectively (r: -0.561, p: 0.01) (r:-0.55 p:0.01). Testicular lengths and weights of the BL groups were smaller compared to CG (p: 0.03),(p: 0.04). GPx was higher for BL-6 and BL-12 than the CG (p:0.021, p:0.024). Testis tissue was compatible with the pubertal period in all groups. As the blue light exposure time increased, spermatogenesis was suppressed, and capillary dilatation and edema in the testis tissue increased.ConclusionOur study is the first to show the effects of blue light exposure on male rats’ puberty process. And we showed that exposure to blue light and the duration of exposure lead to precocious puberty in male rats. The blue light exposure suppressed spermatogenesis, marked vasodilatation in the interstitial area of the testis, and disrupted the integrity of the basement membrane. These findings intensified with increasing exposure time.
Alcohol-induced Cushing syndrome: report of eight cases and review of the literature
Authors: Surani Asif , Carroll Ty B. , Javorsky Bradley R. , Raff Hershel , Findling James W. 
Published: June 2023

IntroductionAlcohol-induced hypercortisolism (AIH) is underrecognized and may masquerade as neoplastic hypercortisolism [Cushing syndrome (CS)] obscuring its diagnosis.Objective and methodsIn order to characterize AIH, we performed a chart review of eight patients (4 males and 4 females; 2014-2022) referred for evaluation and treatment of neoplastic hypercortisolism — six for inferior petrosal sinus sampling, one due to persistent CS after unilateral adrenalectomy, and one for pituitary surgery for Cushing disease (CD). Five underwent dDAVP stimulation testing.ResultsAll eight patients had clinical features of hypercortisolism and plasma ACTH levels within or above the reference interval confirming hypothalamic-pituitary mediation. All had abnormal low-dose dexamethasone suppression test and increased late-night salivary cortisol. Only one had increased urine cortisol excretion. In contrast to CD, the 5 patients tested had blunted or absent ACTH and cortisol responses to desmopressin. Two had adrenal nodules and one had abnormal pituitary imaging. Most patients underreported their alcohol consumption and one denied alcohol use. Elevated blood phosphatidyl ethanol (PEth) was required in one patient to confirm excessive alcohol use. All patients had elevations of liver function tests (LFTs) with AST&gt;ALT.ConclusionAIH is an under-appreciated, reversible cause of non-neoplastic hypercortisolism that is indistinguishable from neoplastic CS. Incidental pituitary and adrenal imaging abnormalities as well as under-reporting of alcohol consumption further confound the diagnosis. Measurement of PEth helps to confirm an alcohol use disorder. Elevations of LFTs (AST&gt;ALT) and subnormal ACTH and cortisol responses to dDAVP help to distinguish AIH from neoplastic hypercortisolism.