bluuebunny/crossref_metadata_2025_split huggingface dataset:
P-541 Does the IVF embryo remain at the site of deposition in successful IVF cycles?
Authors: Vasquez Carlon D , Rouleau J P , Costa M , Hernández J , Naftolin F N , Palumbo A 
Published: June 2023

Abstract

Study question
Does the placement of the IVF embryo differ from the implantation site?

Summary answer
At five weeks the implanted embryo has not moved in the lateral plane of the endometrial cavity but is slightly lower in the vertical plane.

What is known already
We previously showed that vertical placement of embryos approximately 9 mm from the fundus and equidistant from the tubal ostia has the highest implantation rate. Whether the embryo migrates from the point where the droplet is deposited before implantation is controversial. We used 3D ultrasound to compare the point of placement with the location of the gestational sac (GS) at 5 weeks gestational age (GA).

Study design, size, duration
Prospective analysis of 292 transfers of single egg donation blastocysts (2018-2020) and 139 resultant pregnancies in which the position of the GS in the coronal plane at 5 weeks was compared to that of the embryo flash immediately post transfer. A Voluson 6 with a 4D RAB4-8 transabdominal probe imaged the embryo transfer and a 4D RIC-5-9 transvaginal probe imaged the pregnancy scan (GE Healthcare, Austria).

Participants/materials, setting, methods
Consecutive patients with a normal uterine cavity undergoing egg donation in a single private IVF Center. Embryo transfers were performed under 2D US guidance using a soft Wallace Catheter. A 3D volume of the uterus was acquired immediately post-transfer. A second 3D scan was performed at 5 weeks GA. Distances of the embryo flash and the center of GS from the fundus, ostia and internal cervical os (ICO) were recorded and compared using SPSS.

Main results and the role of chance
Of 292 transfer procedures, 139 pregnancies who had the first ultrasound in our Center were included and analysed. The mean distance between the embryo flash and the uterine fundus was significantly less than the distance between the center of the GS and the uterine fundus [(9,49±3,03 vs 11,02±3,9 mm respectively (p &lt; 0,05)]. The mean distances between the embryo flash and the right and left ostium were 16,66 ± 4,96 and 16,69 ± 4,61 mm respectively, with no significant differences from the same distances taken from the center of the GS.

Limitations, reasons for caution
As the image is of the embryo flash and not the embryo, this might have been misleading.

Wider implications of the findings
These findings extend and support the proposal of an optimal point of embryo placement for pregnancy. The apparent vertical difference in the implantation of the embryo could be due to uterine growth or it could be consequence of uterine peristalsis. This bears further study.

Trial registration number
not applicable
P-622 Age-specific reference intervals for anti-müllerian hormone in Mexico: A population-based study. implications for Latin America
Authors: Rodriguez-Purata J , Gingold J , Alonso-de-Mendieta M , Gomez-Cuesta M J , Cervantes-Bravo E 
Published: June 2023

Abstract

Study question
To determine the age-specific normal range of antimullerian hormone (AMH) levels in a female Latin American population.

Summary answer
Age-related nomograms for general population for the 3rd-97th percentiles were produced. These nomograms could provide a reference guide for clinicians to consult women with infertility.

What is known already
The variation of AMH levels among different races and ethnicities has been well documented (Seifer et al., 2009). Despite the wide use of AMH measurements in clinical assessment of ovarian reserve, a population-based estimate for its reference range in Latin America is not available.

Study design, size, duration
A longitudinal, observational, retrospective, real-world study was carried out, including all AMH measurements performed from January 2017 to December 2018 at a large clinical laboratory network in the Mexico City area, encompassing 35 locations. A total of 6444 AMH measurements were included.

Participants/materials, setting, methods
AMH was assayed using either the cobas or ELISA methodologies. All AMH measurements from women 25-45 years were included. The Kolmogorov-Smirnov test was used to confirm that AMH levels between patients assayed with cobas were comparable to those assayed with ELISA at every age.

Main results and the role of chance
In total, 6444 measurements were identified and available for analysis. Age-related normograms for the 3rd, 10th, 25th, 50th, 75th, 90th, and 97th percentiles of AMH were produced. The overall rate of AMH decline accelerated after 40 years of age. In women 25-30 years, 11.4% (63/554) had an AMH &lt;1.0ng/mL. In women 30-35 years, 18.6% (276/1484) had an AMH &lt;1.0ng/mL. In women 35-40 yeras, 35.8% (830/2319) had an AMH &lt;1.0ng/mL. In women 40-45 years, 58.0% (1120/1932) had an AMH &lt;1.0ng/mL.

Limitations, reasons for caution
The lack of medical history for patients precluded identification and exclusión of patients with iatrogenically abnormal AMH levels (e.g. due to oophorectomy). Patients undergoing measurement of AMH were primarily referred through fertility clinics, limiting the potential for extrapolation to the general population.

Wider implications of the findings
The study reports age-specific AMH levels drawn from a large sample of women around Mexico City. The study includes the largest dataset to date. Latin American patients considering fertility preservation should be aware of the high prevalence of diminished ovarian reserve in this large cohort, including among women 35-39.

Trial registration number
Not Applicable
Activation of the Mu-Delta Opioid Receptor Heteromers Blocks Morphine Rewarding Effects
Authors: Requana Aradas Ariadna , Djaboub Youssra , McCort-Tranchepain Isabelle , Hajasova Zuzana , Clémenceau Loïc , Canestrelli Corinne , Mann Anika , Schulz Stefan , Delaval Angélique , Acher Francine , Massotte Dominique , Noble Florence , Marie Nicolas 
Published: June 2023

Abstract

Background
Evidence has accumulated demonstrating the existence of opioid receptor heteromers, and recent data suggest that targeting these heteromers could reduce opioid side effects while retaining therapeutic effects. Indeed, CYM51010 characterized as a MOR (mu opioid receptor)/DOR (delta opioid receptor) heteromer–preferring agonist promoted antinociception comparable with morphine but with less tolerance. In the perspective of developing these new classes of pharmacological agents, data on their putative side effects are mandatory.

Methods
Therefore, in this study, we investigated the effects of CYM51010 in different models related to drug addiction in mice, including behavioral sensitization, conditioned place preference and withdrawal.

Results
We found that, like morphine, CYM51010 promoted acute locomotor activity as well as psychomotor sensitization and rewarding effect. However, it induced less physical dependence than morphine. We also investigated the ability of CYM51010 to modulate some morphine-induced behavior. Whereas CYM51010 was unable to block morphine-induced physical dependence, it blocked reinstatement of an extinguished morphine induced-conditioned place preference.

Conclusions
Altogether, our results reveal that targeting MOR-DOR heteromers could represent a promising strategy to block morphine reward.
What is a Lattice W-Algebra?
Authors: Izosimov Anton , Beffa Gloria Marí 
Published: June 2023

Abstract
We employ the Poisson-Lie group of pseudo-difference operators to define lattice analogs of classical $W_{m}$-algebras. We then show that the so-constructed algebras coincide with the ones given by discrete Drinfeld–Sokolov-type reduction.
Antibody to Plasmodium falciparum Variant Surface Antigens, var Gene Transcription, and ABO Blood Group in Children With Severe or Uncomplicated Malaria
Authors: Barua Priyanka , Duffy Michael F , Manning Laurens , Laman Moses , Davis Timothy M E , Mueller Ivo , Haghiri Ali , Simpson Julie A , Beeson James G , Rogerson Stephen J 
Published: June 2023

Abstract

Background
Antibodies to variant surface antigens (VSAs) such as Plasmodium falciparum erythrocyte membrane protein 1 (PfEMP1) may vary with malaria severity. The influence of ABO blood group on antibody development is not understood.

Methods
Immunoglobulin G antibodies to VSAs in Papua New Guinean children with severe (n = 41) or uncomplicated (n = 30) malaria were measured by flow cytometry using homologous P falciparum isolates. Isolates were incubated with ABO-matched homologous and heterologous acute and convalescent plasma. RNA was used to assess var gene transcription.

Results
Antibodies to homologous, but not heterologous, isolates were boosted in convalescence. The relationship between antibody and severity varied by blood group. Antibodies to VSAs were similar in severe and uncomplicated malaria at presentation, higher in severe than uncomplicated malaria in convalescence, and higher in children with blood group O than other children. Six var gene transcripts best distinguished severe from uncomplicated malaria, including UpsA and 2 CIDRα1 domains.

Conclusions
ABO blood group may influence antibody acquisition to VSAs and susceptibility to severe malaria. Children in Papua New Guinea showed little evidence of acquisition of cross-reactive antibodies following malaria. Var gene transcripts in Papua New Guinean children with severe malaria were similar to those reported from Africa.
Rilmazafone: A designer benzodiazepine pro-drug involved in fatal intoxications
Authors: Kronstrand Robert , Roman Markus , Johansson Anna , Wu Xiongyu , Green Henrik , Truver Michael T 
Published: June 2023

Abstract
Rilmazafone is a pro-drug that can be prescribed in Japan to treat insomnia. Rilmazafone metabolizes into active compounds by a ring closure resulting in a triazolo benzodiazepine structure similar to alprazolam. In mid-2022, the National Board of Forensic Medicine in Sweden were requested to investigate two separate deaths with the suspected use of pagoclone. Packages labeled “Pagoclone” were found at each scene that was suspected to contain rilmazafone based on website information. During screening by high resolution mass spectrometry, rilmazafone metabolites were presumptively identified. Due to the lack of reference material for the active metabolites, the metabolites were synthesized in house and quantification of the compounds identified in the two autopsy cases was prompted. In Case 1, femoral blood concentrations of 7.9, 65 and 170 ng/g of the metabolites rilmazolam, N-desmethyl rilmazolam and di-desmethyl rilmazolam, respectively, were detected. Additional toxicological findings included the medications haloperidol, alimemazine, fluoxetine, olanzapine and acetaminophen. In Case 2, femoral blood concentrations of 1.7, 1.4 and 70 ng/g of rimazolam, N-desmethyl rilmazolam and di-desmethyl rilmazolam, respectively, were detected. Additional toxicological findings included loperamide, alimemazine and pregabalin. The intake of rilmazafone was determined as the cause of death in Case 1 and contributed in the Case 2.
Hot Earth or Young Venus? A nearby transiting rocky planet mystery
Authors: Kaltenegger L , Payne R C , Lin Z , Kasting J , Delrez L 
Published: June 2023

ABSTRACT
Venus and Earth provide astonishingly different views of the evolution of a rocky planet, raising the question of why these two rocky worlds evolved so differently. The recently discovered transiting Super-Earth LP 890-9c (TOI-4306c, SPECULOOS-2c) is a key to the question. It circles a nearby M6V star in 8.46 d. LP890-9c receives similar flux as modern Earth, which puts it very close to the inner edge of the Habitable Zone (HZ), where models differ strongly in their prediction of how long rocky planets can hold onto their water. We model the atmosphere of a hot LP890-9c at the inner edge of the HZ, where the planet could sustain several very different environments. The resulting transmission spectra differ considerably between a hot, wet exo-Earth, a steamy planet caught in a runaway greenhouse, and an exo-Venus. Distinguishing these scenarios from the planet’s spectra will provide critical new insights into the evolution of hot terrestrial planets into exo-Venus. Our model and spectra are available online as a tool to plan observations. They show that observing LP890-9c can provide key insights into the evolution of a rocky planet at the inner edge of the HZ as well as the long-term future of Earth.
Clinical features of newly diagnosed systemic lupus erythematosus after SARS-CoV-2 vaccination
Authors: Moriyama Masayori , Noda Kentaro , Ito Haruyasu , Matsushita Takayuki , Kurosaka Daitaro 
Published: June 2023

ABSTRACT
Systemic lupus erythematosus (SLE) is an autoimmune disease that causes damage to multiple organs. Various factors, including vaccination, have been associated with SLE development. Vaccination for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) began in 2020, and there are a few reports on the exacerbation of SLE after SARS-CoV-2 vaccination. The influence of SARS-CoV-2 vaccination on SLE development remains unclear. We present the case of a 53-year-old man who developed peritonitis and was subsequently diagnosed with SLE on Day 9 after receiving a third dose of the messenger ribonucleic acid-1273 SARS-CoV-2 vaccine. This case and previous reports have shown that patients who developed SLE after SARS-CoV-2 vaccination are more likely to develop it within 2 weeks of vaccination, especially when they have a higher rate of immunological abnormalities or a family history of autoimmune diseases. Furthermore, these features suggest that type I interferon is involved in the pathogenesis of SLE after SARS-CoV-2 vaccination.
Development of “Baby, Me, & NRT”: A Behavioral Intervention to Improve the Effectiveness of Nicotine Replacement Therapy in Pregnancy
Authors: McDaid Lisa , Emery Joanne , Thomson Ross , Coleman Tim , Cooper Sue , Dickinson Anne , Kinahan-Goodwin Darren , Phillips Lucy , Naughton Felix 
Published: June 2023

Abstract

Background
The effectiveness of Nicotine Replacement Therapy (NRT) for smoking cessation in pregnancy is limited by inconsistent and incorrect use. This paper describes the development process for “Baby, Me, & NRT”, a novel pregnancy-specific intervention aimed at enhancing adherence to NRT.

Methods
An integrated approach to intervention development was used, combining evidence, theory, stakeholders’ feedback, and tailoring principles. The process involved six iterative steps: (1) synthesizing relevant published evidence and guidance, (2) collecting primary qualitative data on barriers and facilitators to NRT adherence along with potential intervention design features, (3) identifying relevant behavioral theories and mapping the evidence against these, (4) prioritizing behavioral determinants identified in steps 1 and 2, generating intervention objectives, and identifying behavior change techniques which target the prioritized determinants, (5) consulting with stakeholders on intervention components, key content and tailoring features, and (6) producing a prototype intervention along with implementation guidance.

Results
The prototype intervention comprises of a multi-component, 1-month cessation programme, which includes six enhanced behavioral support sessions delivered by a trained advisor, tailored text messages, a website, and an illustrated booklet. It promotes the uptake of high-dose and combination NRT, emphasizes the importance of adherence, addresses motivation to use NRT, proactively helps problem solve NRT use issues, and provides guidance on preventing and managing smoking lapses.

Conclusion
The development process generated an evidence- and theory-guided intervention, designed with stakeholder input, aimed at improving NRT effectiveness for smoking cessation in pregnancy. The prototype intervention has since been optimized and is being evaluated in a randomized controlled trial.
Genome Sequence Resource of Burkholderia gladioli KRS027: A Strain with Antifungal Activity Against Plant Pathogens
Authors: Wang Dan , Zhang Dan-Dan , Li Ran , Zhu He , Song Jian , Kong Zhi-Qiang , Dai Xiao-Feng , Han Dongfei , Chen Jie-Yin 
Published: June 2023

Some species of Burkholderia have been studied extensively for their ability to control plant diseases and promote plant growth. Burkholderia gladioli KRS027 is a strain isolated from the rhizosphere soil of a healthy plant in a pathogen-infested cotton field in Xinjiang, China, which had an inhibitory ability against various plant-pathogenic fungi. To understand its biocontrol mechanism, we assembled a high-quality genome of KRS027 using the PacBio-HiFiReads. The KRS027 genome contained two circular DNA chromosomes and one plasmid, of which Chr1 contained 4,187,756 bp with 67.95% GC content, Chr2 had 3,892,275 bp with 68.56% GC content, and the plasmid comprised 177,327 bp with 61.11% GC content. Moreover, the genome contains 20 secondary metabolite biosynthesis gene clusters involving polyketides (PKS), nonribosomal peptides (NRPS), hybrid NRPS/PKS, and terpene. The genes involved in biosynthesis of phytotoxins, such as toxoflavin, rhizotoxin, and bongkrekic acid, were not detected. The high-quality genome resource of strain KRS027 will provide a molecular basis for further research on its biocontrol mechanisms. [Formula: see text] Copyright © 2023 The Author(s). This is an open access article distributed under the CC BY-NC-ND 4.0 International license .
Targeting mitochondrial dysfunction using methylene blue or mitoquinone to improve skeletal aging
Authors: Poudel Sher Bahadur , Frikha-Benayed Dorra , Ruff Ryan R. , Yildirim Gozde , Dixit Manisha , Korstanje Ron , Robinson Laura , Miller Richard A. , Harrison David E. , Strong John R. , Schaffler Mitchell B. , Yakar Shoshana 
Published: June 2023

AbstractDeclines in mitochondrial content and impaired cytochrome c oxidase activity (complex IV) can result in reduced energy metabolism and increased levels of oxidants. Methylene blue (MB) is a well-established antioxidant that has been shown to improve mitochondrial function in both in vitro and in vivo settings. Mitoquinone (MitoQ) is a selective antioxidant that specifically targets mitochondria and effectively reduces the accumulation of reactive oxygen species (ROS) within cells.To investigate the effect of acute and long-term administration of MB on skeletal morphology, we administered MB to aged (18 months old) female C57BL/J6 mice, as well as to adult male and female mice with a genetically diverse background (UM-HET3). Additionally, we used MitoQ as an alternative approach to target mitochondrial oxidative stress during aging in adult female and male UM-HET3 mice.Although we observed some beneficial effects of MB and MitoQ in vitro, the administration of these compounds in vivo did not alter the progression of age-induced bone loss. Specifically, treating 18-month-old female mice with MB for 6 or 12 months did not have an effect on age-related bone loss. Similarly, long-term treatment with MB from 7 to 22 months or with MitoQ from 4 to 22 months of age did not affect the morphology of cortical bone at the mid-diaphysis of the femur, trabecular bone at the distal-metaphysis of the femur, or trabecular bone at the lumbar vertebra-5 (L5) in UM-HET3 mice.Based on our findings, it appears that long-term treatment with MB or MitoQ alone, as a means to reduce skeletal oxidative stress, is insufficient to inhibit age-associated bone loss. This supports the notion that interventions solely with antioxidants may not provide adequate protection against skeletal aging.
Hybrid machine-learning framework for volumetric segmentation and quantification of vacuoles in individual unlabeled yeast cells using holotomography
Authors: Lee Moosung , Kunzi Marina , Neurohr Gabriel , Lee Sung Sik , Park Yongkeun 
Published: June 2023

AbstractThe precise, quantitative evaluation of intracellular organelles in three-dimensional (3D) imaging data poses a significant challenge due to the inherent constraints of traditional microscopy techniques, the requirements of the use of exogenous labeling agents, and existing computational methods. To counter these challenges, we present a hybrid machine-learning framework exploiting correlative imaging of 3D quantitative phase imaging with 3D fluorescence imaging of labeled cells. The algorithm, which synergistically integrates a random-forest classifier with a deep neural network, is trained using the correlative imaging data set, and the trained network is then applied to 3D quantitative phase imaging of unlabeled cell data. We applied this method to unlabeled live budding yeast cells. The results revealed precise segmentation of vacuoles inside individual yeast cells, and also provided quantitative evaluations of biophysical parameters, including volumes, concentration, and dry masses of automatically segmented vacuoles.
Absolute calibration of ribosome profiling assesses the dynamics of ribosomal flux on transcripts
Authors: Tomuro Kotaro , Mito Mari , Toh Hirotaka , Kawamoto Naohiro , Miyake Takahito , Chow Siu Yu A. , Doi Masao , Ikeuchi Yoshiho , Shichino Yuichi , Iwasaki Shintaro 
Published: June 2023

AbstractRibosome profiling, which is based on deep sequencing of ribosome footprints, has served as a powerful tool for elucidating the regulatory mechanism of protein synthesis. However, the current method has substantial issues: contamination by rRNAs and the lack of appropriate methods to determine overall ribosome numbers in transcripts. Here, we overcame these hurdles through the development of “Ribo-FilterOut”, which is based on the separation of footprints from ribosome subunits by ultrafiltration, and “Ribo-Calibration”, which relies on external spike-ins of stoichiometrically defined mRNA-ribosome complexes. A combination of these approaches measures the absolute number of ribosomes on a transcript, the translation initiation rate, and the overall number of translation events before its decay, all in a genome-wide manner. Moreover, our method revealed the allocation of ribosomes under heat shock stress, during aging, and across cell types. Our strategy transforms ribosome profiling technique from relative to absolute quantification of translation.
The F pilus serves as a conduit for the DNA during conjugation between physically distant bacteria
Authors: Goldlust Kelly , Ducret Adrien , Halte Manuel , Dedieu-Berne Annick , Erhardt Marc , Lesterlin Christian 
Published: June 2023

AbstractHorizontal transfer of F-like plasmids by bacterial conjugation is responsible for disseminating antibiotic resistance and virulence determinants among pathogenic Enterobacteriaceae species, a growing health concern worldwide. Central to this process is the conjugative F pilus, a long extracellular filamentous polymer that extends from the surface of plasmid donor cells, allowing it to probe the environment and make contact with the recipient cell. It is well established the F pilus can retract to bring mating pair cells in tight contact before DNA transfer. However, whether DNA transfer can occur through the extended pilus has been a subject of active debate. In this study, we use live-cell microscopy to show that the F pilus can indeed serve as a conduit for the DNA during transfer between physically distant cells. Our findings enable us to propose a new model for conjugation that revises our understanding of the DNA transport mechanism and the dissemination of drug resistance in complex bacterial communities.One-Sentence Summary:Plasmid DNA passes through the F pilus during conjugational transfer between physically distant bacteria.