bluuebunny/crossref_metadata_2025_split huggingface dataset:
Microbiota changes in a stranding simulation of the holopelagic macroalgae Sargassum natans and Sargassum fluitans
Authors: Mendonça Inara R. W. , Theirlynck Tom , Zettler Erik R. , Amaral-Zettler Linda A. , Oliveira Mariana Cabral 
Published: February 2023

Abstract

Holopelagic Sargassum has been causing massive strandings on tropical Atlantic Ocean shorelines. After stranding, the algal biomass starts to decompose, releasing nutrients, toxic gases, and potentially introduces exogenous macro and microorganisms. Describing the microbiome associated with Sargassum, and how it changes after stranding is important in identifying potential microbial introductions to coastal environments, as well as sources of potential biotechnological resources. In this study, stranding simulation experiments were done for S. fluitans III and S. natans VIII on shipboard. Samples for microbiome identification were taken at 0 hr, just after removing healthy Sargassum from the seawater, and after 24 and 48 hrs of stranding simulation under environmental conditions. The bacterial community was identified through sequencing of 16S rRNA gene V3-V4 hypervariable regions, generating a total of 2,005 Amplicon Sequence Variants (ASVs). Of those, 628 were shared between Sargassum species. The stranding simulation changed the microbial community and only 30, out of 2,005 ASVs, persisted throughout the experiment. Phototrophs were in the main functional group at 0 hr, shifting to chemoheterotrophs within the first 24 hrs of exposure of Sargassum to air conditions. The most abundant orders Microtrichales and Rhodobacterales at 0 hr, were replaced after 24 hrs of exposure by Alteromonadales and Vibrionales, the latter representing up to 91% of the relative abundance in the bacterial community. These findings suggest that after stranding, the Sargassum microbiome goes through dysbiosis, and its biomass could become a fertile ground for potentially pathogenic bacteria.
Intrinsec Epidermoid Cyst of the Brainstem in Children – Case Report and Review
Authors: Malveira Adib Saráty , Costa Marcos Devanir Silva , Flores Esdras Ismael Borrayo , Vaz Herisson Harrider Silva , Nicácio Jardel Mendoça , Cavalheiro Sérgio 
Published: February 2023

Abstract
Purpose
The study aimed to summarize all published cases of intrinsic brainstem epidermoid cysts in a timeline to highlight the specific characteristics and individualize the disease, in addition to discussing the best treatment used.
Methods
The scientific literature on pediatric cases of intrinsic epidermoid cysts of the brainstem was analysed. We present the case of a1,5-year-old male with incidental apresentation, who was treated with gross total resection. We summarize all previously published cases to individualize the disease.
Results
We identified 21 patients, including 10 boys and 11 girls, with a mean age of 4.85 (1–15) years at the time of surgery. The most frequent symptoms were cranial nerve palsy (71.4%), pyramidal tract deficit (57.14%) and headache (52.38%). Among the affected cranial nerves, VII was the most frequent, reported in 10 patients.
Conclusion
Brainstem epidermoid cysts are extremely rare pathologies with relevant age involvement in young children. The treatment objective should be the maximum resection of the
In silico studies of Cilnidipine oxidative degradants for structure confirmation, toxicity prediction and molecular docking
Authors: Chintalapati Krishnam Raju , Kada Yesudas , Malkhed Vasavi , Palusa Sanath Kumar Goud , Bera Rabin , Jagarlapudi V Shanmukha Kumar 
Published: February 2023

AbstractOxidative degradation products (KD1 to KD4 and CD1 to CD3) of Cilnidipine has been isolated and characterized using Mass, FTIR and NMR studies in the reported article. The elucidated structures of CD1 and CD2 are shown 5 chiral centers each against one chiral center of API. The CD1 and CD2 structures could exhibit 16 enantiomeric pairs for each. NMR experimental data confirmed one enantiomer pair out of the possible isomers. Due to the complexity for structure confirmation of CD1 and CD2. In the current study, revisited the elucidated structures of CD1 and CD2 and reconfirmed using computational studies. NMR chemical shifts of hydrogen and carbon atoms of CD1 and CD2 are theoretically generated using the density functional theory (DFT) in DMSO. These values were compared with experimentally obtained chemical shift values. The results are in good agreement with the DFT calculations in terms of the chemical shifts and the stereo-configurations as well. Docking studies are carried out to identify the interactions and mode of binding of the Cilnidipine and their degradation products (KD1 to KD4 and CD1 to CD3) with the N-type Calcium channel subunit. Additionally, 20 drugs acting as Calcium channel blockers are also considered for docking analysis to correlate the affinities of binding. The interactions reveal the amino acid residues which are involved in binding. CD3 molecule shows the highest bind affinity with the ligand molecules with binding energy -9.3 (kcal/mol) and then KD3 with binding energy -8.7 (kcal/mol). Conducted in-silico toxicity predictions for Cilnidipine and their degradation products (KD1 to KD4 and CD1 to CD3). Two complementary methodologies; rule-based and statistical-based methodologies are applied to predict the bacterial mutagenicity and classified the impurities in accordance with ICH M7. The Alerting group is observed in CD1 to CD3 and no alerting group is observed in KD1 to KD4 for bacterial mutagenicity prediction. Based on in-silico toxicity predictions and docking studies, KD3 can be considered as a potential compound for binding with receptor molecule.
CSNK2A1/AKT1 signal axis plays a crucial role in DUSP2-mediated apoptosis in pancreatic cancer
Authors: Zhang Yangyang , Kong Rui , Yang Wenbo , Hu Keyi , Zhao Zhongjie , Li Le , Geng Xinglong , Liu Liwei , Chen Hongze , Xiao Peng , Liu Danxi , Luo Yan , Chen Hua , Hu Jisheng , Sun Bei 
Published: February 2023

Abstract
Pancreatic cancer is a malignant tumor of the digestive tract with poor prognosis. Dual-specificity phosphatase 2 (DUSP2) is a member of the mitogen-activated protein kinase phosphatase family that is involved in multiple pathophysiological processes. However, its role in pancreatic cancer remains further investigation. The most prominent feature of pancreatic cancer is its hypoxic microenvironment, which plays an important role in tumor progression, drug resistance, and immune evasion. Therefore, we explored the role of DUSP2 by simulating such tumor microenvironment and found that DUSP2 regulated the apoptosis of pancreatic cancer in vitro and in vivo. Mechanistically, DUSP2 competed with AKT1 for binding with casein kinase 2 alpha 1 (CSNK2A1) to inhibit the phosphorylation of AKT1, which played a crucial role in regulating apoptosis. Interestingly, aberrant activation of AKT1 resulted in an increase in the ubiquitin E3 ligase tripartite motif containing 21 (TRIM21), which could bind to and mediate ubiquitination-dependent proteasomal degradation of DUSP2. Taken together, our findings demonstrate a novel signal axis of CSNK2A1/AKT1 in DUSP2 regulating apoptosis and the existence of AKT1/TRIM21 feedback regulation in the degradation of DUSP2 protein in pancreatic cancer for the first time.
Nonlinear optical properties of BiWO4
Authors: Sharath  , Rahulan Mani , PrakashBabu  
Published: February 2023

Abstract
We report the third order nonlinear optical properties of BiWOO4 nanostructures,. BiWO4 nanostructures with different concentration of Er were synthesized by chemical precipitation method and were characterized by UV–Vis absorption, X-ray diffraction (XRD), transmission electron microscopy (TEM) and fluorescence measurements. The incorporation of Er ions shifted the absorption band of BiWO4 towards higher wavelength and enhanced the light absorption in the visible region. XRD patterns showed that the powders crystallize in scheelitetype tetragonal structure. The nonlinear optical behavior of the nanostructures was investigated by a Z-scan technique at 532 nm using continuous wave Nd:YAG laser. Experimental results suggested that the addition of Er can considerably enhance the nonlinear absorption coefficient and refractive index of BiWO4 which could be used as a potential for nonlinear optical device applications.
Procyanidins Extracted from the Lotus Seedpod Ameliorate Cognitive
Impairment through CREB-BDNF Pathway Mediated LTP in APP/PS1
Transgenic Mice
Authors: Wang Ziping , Li Xiaolong , Ren Xiaotong , Zhao Siqi , Chen Wenwen , Fan Cheng , Xu Yangliu , Pi Xuejiao , Zhang Yidan , Wang Ting , Rong Shuang 
Published: February 2023

Background:
Alzheimer’s disease (AD) is an age-related neurodegenerative disease
and is featured by cognitive impairment. Procyanidins have been shown to have a potential protective
effect against neurodegenerative diseases, but the underlying mechanism is not comprehensive
enough.

Objective:
To further investigate the effects of procyanidins from lotus seedpod (LSPC) on cognition
in AD.

Methods:
The APP/PS1 transgenic mice were administered with LSPC (100 mg/kg body weight)
for five months. The Morris water maze test was used to assess learning and memory function, the
long-term potentiation (LTP) was measured, and the expressions of Aβ, pCREB/CREB and BDNF
were quantified by western blot.

Results:
LSPC significantly ameliorated cognitive dysfunction, reduced Aβ deposition and reversed
the remarkable reduction of the phosphorylation of CREB and the expression of BDNF,
and then enhanced the effect of LTP in APP/PS1 mice.

Conclusion:
These results revealed that LSPC could ameliorate cognitive impairment through the
CREB-BDNF pathway that mediates the enhancement of LTP in APP/PS1 transgenic mice.
Analisis kandungan teaflavin dan tearubigin pada teh hitam yang diolah melalui metode pelayuan kimia bertahap
Authors: Shabri Shabri , Rohdiana Dr. Dadan , Maulana Hilman , Haryanto Sugeng , Prawira-Atmaja Muhammad Iqbal 
Published: February 2023

The instability of tea shoots supply from the plantation to the factory was often a problem. In the flush season where tea shoots are abundant, an overcapacity often leads to the uncontrollable withering process. This problem was the cause of the decreased quality of black tea. Theaflavin (TF) and Thearubigin (TR) were chemical substances that positively correlated to the black tea quality. This research conducted to characterize TF and TR quality in two-stages chemical withering. This research treatment consists of two treatments, namely treatment A (plus season), treatment B (minus season), and one control/routine production (treatment C). Treatment A and B used 25 kg of fresh tea shoots to be withered, the first stage was to wither the leaves until it reached 60%, 65%, and 70% of water content. The second stage was to blended those leaves at the first stage and withered it until it reached about 54-56%. For treatment A and B, the enzymatic oxidation (fermentation) time used 90 minutes. Treatment C used 25 kg of fresh tea shot that processed as normal routine production of black tea orthodox-rotor vane process, with enzymatic oxidation time 110 minutes. Every treatment was repeated 3 times. Treatment A produced TF of 0,97% d.b and TR of 15,39 d.b. (ratio 1:15,97). And treatment C produced TF of 0,92% d.b and TR of 13,69% d.b. (ratio 1:14,82). It was concluded that two-stage chemical withering was able to maintain the chemical and organoleptic qualities of black tea.
Karakteritik sifat alir bubuk teh hijau yang diproses dengan metode penepung berbeda
Authors: Prawira-Atmaja Mukhammad Iqbal , Haryanto Sugeng , Maulana Hilman , Shabri Shabri , Rohdiana Dr. Dadan 
Published: February 2023

Recently, the public interest in green tea powder is increasing. Green tea powder is produced through the process stage: withering in hot steam, drying, and reducing size. The size reduction process on green tea powder is an important step because it will affect the characteristics of the powder flow properties. This study aims to determine the flow properties of green tea powder processed by different milling method. The milling method used are disc milling (DM) and stone milling (SM). Green tea powder was analyzed including moisture content, particle size distribution using dynamic laser scattering method, determined of bulk density (ρb), and tapped density (ρtap) to find out the Carr Index (CI) and Hausner Ratio (Hr). In addition, the lightness of color and shape and morphology of green tea powder were observed with SEM. The results showed that green tea powder produced from different milling methods have a Carr Index of 15 with “Good” flow properties. DM method produced darker green tea powder with L value (lightness) 49.91 and larger particle size (D50: 38.6 µm) compared with green tea powder produced by the SM method. Determination of flow properties of green tea powder can be applied for the utilization and development of food products and pharmaceutical products based on green tea powder.
Analisis efektivitas tenaga kerja pada stasiun kerja sortasi kering teh hijau menggunakan metode overall labor effectiveness (OLE) di PPTK
Authors: Anggara Bayu 
Published: February 2023

Ketatnya persaingan industri pada era sekarang ini, semakin memacu perusahaan manufacturing untuk terus melakukan perbaikan demi keberlanjutan perusahaan. Permintaan pasar harus dapat dipenuhi perusahaan sebagai upaya menjaga kepercayaan dari konsumen. Usaha yang perlu dilakukan perusahan untuk meningkatkan produktivitas diantaranya dengan meningkatkan efesiensi tenaga kerja. Penelitian ini bertujuan untuk mengidentifikasi tingkat efektivitas tenaga kerja dan mengidentifikasi kemungkinan yang dapat menurunkan nilai OLE. pada stasiun kerja sortasi kering di Pabrik Teh Hijau PPTK. Penelitian ini menggunakan metode Overall Labor Effectiveness (OLE) dan diagram fishbone. Indikator yang dihitung meliputi availability, performance, dan quality yang diamati selama enam hari (13-18 Januari 2020) pada stasiun kerja sortasi kering. Hasil identifikasi menunjukkan bahwa nilai OLE yaitu 55,77%. Nilai ini berada dibawah standar dunia yaitu 85%. Setelah diindentifikasi kemungkinan penyebab masalah yang sangat mempengaruhi nilai OLE yaitu : 1) Berdasarkan pengukuran selama 6 hari yaitu rata-rata mengalami keterlambatan bahan selama 90 menit dan 2) Kekurangan mesin fibro sehingga menyebabkan penumpukan bahan pada mesin tersebut.
Analisis postur kerja di stasiun sortasi kering pabrik teh hijau PPTK Gambung dengan metode REBA (Rapid Entire Body As-sessment)
Authors: Timur Yudhisthira 
Published: February 2023

Tuntutan kerja yang tinggi sering membuat pekerja mengabaikan kenyamanan dan keselamatan, sehingga kecelakaan kerja sering terjadi di pabrik, seperti di stasiun sortasi kering Pusat Penelitian Teh dan Kina (PPTK). Oleh sebab itu, penelitian ini dilakukan untuk 1) menganalisis keluhan Musculoceletal Disorders (MSDs) dengan Nordic Body Map (NBM) dan 2) menganalisis postur kerja dengan metode REBA. Penelitian ini dilakukan pada 13-20 November 2020 di stasiun sortasi kering PPTK. Hasil yang diperoleh penelitian ini antara lain 1) diketahui bahwa karakteristik pekerja tidak selalu berpengaruh pada keluhan MSDs, 2) bagian tubuh yang paling sering dikeluhkan paling sakit adalah lengan bawah kanan, dan 3) sebagian besar pekerja memiliki skor NBM yang kecil. Pada analisis postur keja dengan metode REBA, diketahui bahwa kegiatan-kegiatan seperti memindahkan karung ke mesin midleton, memindahkan karung dari suatu mesin ke mesin lain dengan satu tangan, dan mengangkat karung dari bawah ke atas (pada mesin dengan corong di atas) memiliki skor REBA yang tinggi sehingga disarankan untuk segera dilakukan penyelidikan lebih lanjut dan perbaikan segera.
PERAN BADAN KEPENDUDUKAN DAN KELUARGA BERENCANA NASIONAL (BKKBN) DALAM PERCEPATAN PENURUNAN STUNTING PERSPEKTIF PERPRES NOMOR 72 TAHUN 2021
Authors: Lailiyah Kusroh 
Published: February 2023

This study aims to determine the role of the BKKBN in accelerating the reduction of the stunting rate in Indonesia and the efforts made by the BKKBN in accelerating the reduction of the stunting rate in terms of Presidential Regulation (Perpres) Number 72 of 2021 which is the legal basis for implementing the stunting reduction program in Indonesia. This study uses a qualitative approach through descriptive analysis method based on the study of the literature obtained and then used as material for analysis. The results of this study indicate that the BKKBN has a very important role in reducing stunting rates in Indonesia. This is because the BKKBN was appointed as the implementing coordinator in the stunting reduction program as stipulated in article 15 paragraph 2 of Presidential Decree Number 72 of 2021. The task of the BKKBN is to coordinate, synergize, and evaluate the implementation of the Acceleration of Stunting Reduction in an effective, convergent and integrated manner by involving cross-sectors in central and regional levels. In an effort to accelerate the reduction of stunting rates, the BKKBN as the chief executive has issued a National Action Plan to Accelerate the Reduction of Stunting Rates in Indonesia (RAN PASTI) as a reference in implementing the acceleration of stunting reduction for Ministries and Agencies, Provincial Governments, District/City Governments, Village Governments, as well as other stakeholders. The framework used in RAN PASTI focuses on three (3) approaches, namely the nutrition intervention approach, the multi-sector and multi-stakeholder approach, and the family-based approach at risk of stunting.
Keywords: Stunting, Presidential Decree No. 72 of 2021, BKKBN, stunting reduction
Analisis Manajemen Risiko Rantai Pasok Pada Produk Alat Berat Penunjang Industri Pertambangan
Authors: Atho R Muhamad , Hasibuan Sawarni 
Published: February 2023

Manufacturing activities such as trailer products potentially generate various risks. This research aims to analyze risk management in the supply chain of trailer production using the House of Risk (HOR) model. The business activities mapping is using the Supply Chain Operations Reference (SCOR) method which consists of 6 parts of the management process, plan, source, make, deliver return and enable. Research is conducted through two phases of HOR. The HOR 1 activities are the identification of risk events and risk agents based on SCOR mapping, measurement of severity, and occurrence levels to produce an Aggregate Risk Priority (ARP), then determine a priority risk agent in the mitigation process. The HOR 2 is used to determine which actions are to be done ﬁrst, considering their differing effectiveness as well as resources involved and the degree of difﬁculties in performing. The company should ideally select a set of actions that are not so difﬁcult to perform but could effectively reduce the probability of risk agents occurring. 7 experience respondents were involved in the study consisting of engineering, procurement, production, warehouse, production planning, after-sales dan quality control. The results of the analysis successfully identified 46 risk events and 41 risk agents in the manufacturing of mining trailers. FGD and Pareto analysis used in the study produced 16 recommendations for mitigation actions that are expected to be able to mitigate risk.
Kejujuran Akademik Mahasiswa dan Persepsi Anti Korupsi
Authors: Heryadi Adi , Jayanti Arini Mifti , Zetta Cantika Vanessa Emma 
Published: February 2023

Penelitian Suud dan Subandi (2017) mengatakan bahwa korupsi yang terjadi salah satunya adalah dampak dari ketidakjujuran. Mahasiswa yang notabene adalah kader pemimpin bangsa masa depan perlu terus ditanamkan nilai-nilai kejujurannya dibidang akademik sebagai bagian dari upaya mencetak pemimpin Indonesia 2045 yang bebas korupsi. Persepsi anti korupsi adalah cara pandang individu atau pemrosesan lebih lanjut dari nilai-nilai anti korupsi yang didapatkan melalui pengalaman sehingga kita dapat menyadari sesuatu yang benar, baik dan membangun untuk tidak mendukung upaya yang merugikan negara (Triana & Heryadi, 2020). Penelitian ini bertujuan untuk melihat hubungan antara kejujuran akademik mahasiswa dengan persepsi anti korupsi . Sampel dalam penelitian ini berjumlah 194 orang mahasiswa dari 4 program studi di Fakultas Ekonomi dan Sosial Unjani Yogyakarta dan didapatkan dengan tehnik insidental sampling. Alat Ukur yang digunakan dalam penelitian ini adalah skala kejujuran akademik yang dimodifikasi dari skala Utami, dkk (2020) yang setelah diujicobakan memiliki nilai Alpha Cronbach (α) 0,945 dengan 59 aitem sahih dan skala persepsi anti korupsi memodifikasi skala Triana dan Heryadi (2020) yang setelah diujicobakan memiliki nilai Alpha Cronbach (α) 0,814 dengan 28 aitem sahih. Hasil uji hipotesis statistic product moment diperolah hasil koefisien korelasi 0,648;p =0,000 (p<0,05), artinya terdapat hubungan yang signifikan antara persepsi anti korupsi dan kejujuran akademik mahasiswa dan arah hubungan bersifat positif..
Development of insulation moisture level criteria as a result of technological progress in the maintenance system of oil-filled transformers
Authors: Vysogorets S. P. , Nesterenko D. M. , Lopatin V. V. , Redkin S. M. 
Published: February 2023

Transformers are critical elements of electrical networks and systems. The operational reliability of oil-filled transformers largely depends on the characteristics of the insulation, which is confirmed by the failure statistics. A number of factors affecting the insulation of electrical machines are identified, including excessive moisture, with its negative consequences outlined. The complexity and shortcomings of the use of traditional technologies for drying solid insulation are indicated, which focus on the search and implementation of the latest technologies in the system of maintenance and repair of transformers — automated insulation restoration systems (AIRS).Taking into account the development of technical progress in the field of service maintenance of transformers, the negative impact of moisture on the reliability, durability and overload capacity of electrical machines, especially those beyond their normal service life, a new look at the criteria for the moisture content of cellulose insulation is proposed. A review is made of the normative requirements for the moisture content of the insulation of oil-filled transformers of a number of Russian and foreign standards. On the basis of the Oommen equilibrium curves, the shortcomings of the lack of comparability of the humidity criteria for solid and liquid insulation in STO 34.01–23.1– 001–2017 and RD 34.45–51.300–97 are presented. It is proposed to single out several areas of the operational state of transformers in terms of “moisture content of solid insulation (paper)”. The optimal period for the use of AIRS has been determined.The AIRS technology is implemented in a TRANSEC module, which enables to carry out work under load, without adversely affecting predictive diagnostics. The design features of the TRANSEC module, the principle of operation, functional and design differences from standard transformer units – thermosiphon /adsorption filters — are outlined. The results of testing the TRANSEC technology in an existing electrical installation serviced by PJSC Rosseti are presented. Six months of trial operation of the TRANSEC module demonstrated the restoration of insulation characteristics on operating equipment without additional involvement of personnel. Besides, during the testing of the TRANSEC technology, the influence of oil temperature on the information content of the assessment of solid insulation parameters was confirmed. Proposals are formulated on the revision of industry documentation in the established part.