bluuebunny/crossref_metadata_2025_split huggingface dataset:
PI3 expression predicts recurrence after chemotherapy with DNA‐damaging drugs in gastric cancer
Authors: Harada Kenji , Sakamoto Naoya , Kitaoka Takumi , Nakamura Yuka , Kondo Ryotaro , Morisue Ryo , Hashimoto Hiroko , Yamamoto Yusuke , Ukai Shoichi , Maruyama Ryota , Sakashita Shingo , Kojima Motohiro , Tanabe Kazuaki , Ohdan Hideki , Shitara Kohei , Kinoshita Takahiro , Ishii Genichiro , Yasui Wataru , Ochiai Atsushi , Ishikawa Shumpei 
Published: February 2025

AbstractDespite recent advances in gastric cancer therapy, chemotherapy resistance and lack of methods for selecting combination regimens remain major problems. Organoids, which provide a culture system that more closely resembles tumor cell organization than traditional cell lines, can be established from surgical specimens with a high success rate and are widely used for drug sensitivity assays. In this study, we aimed to identify a novel biomarker for predicting multidrug resistance using gastric cancer organoids (GCOs). We evaluated 5‐fluorouracil or oxaliplatin‐resistant GCOs to find novel biomarkers that reflect multidrug resistance in gastric cancer. To examine the resistance mechanisms, RNA‐sequencing analysis and ex vivo drug sensitivity testing were performed. The association of biomarkers with patient prognosis and chemotherapy efficacy was evaluated using three original cohorts with a total of 230 cases. The results were also validated with two independent public cohorts and single‐cell RNA sequence data. Increased expression of peptidase inhibitor 3 (PI3) was detected in all 5‐fluorouracil or oxaliplatin‐resistant GCOs. Our findings suggest a potential association of PI3 expression with ribosome biosynthesis and RNA metabolism under organoid conditions. We also found that PI3 overexpression promoted 5‐fluorouracil/oxaliplatin/cisplatin resistance but not paclitaxel resistance. Immunohistochemical evaluation of PI3 expression revealed that the PI3‐positive gastric cancer group had a poorer outcome, especially in terms of time to recurrence. PI3 positivity was also an independent predictor of relapse after chemotherapy with DNA‐damaging agents. PI3 promotes DNA‐damaging drug resistance through multiple downstream regulations related to RNA and ribosomal metabolism. PI3 may be useful as a biomarker for the therapeutic selection of non‐DNA‐damaging agents. © 2025 The Author(s). The Journal of Pathology published by John Wiley & Sons Ltd on behalf of The Pathological Society of Great Britain and Ireland.
Spatial profiling of ANO7 in prostate tissue: links to AR‐signalling‐associated lipid metabolism and inflammation
Authors: Metsälä Olli , Wahlström Gudrun , Goel Neha , Miihkinen Mitro , Taimen Pekka , Schleutker Johanna 
Published: February 2025

AbstractProstate cancer (PrCa) is highly prevalent in the Western world. Currently, however, there are many unmet needs in PrCa care, for example in distinguishing between clinically significant and indolent cases in early phases of the disease. ANO7 is a prostate‐specific gene associated with PrCa risk and prognosis, but its exact function in the prostate remains unclear. This study investigates the role of ANO7 in benign prostatic epithelium using spatial transcriptomics by examining differences between ANO7‐expressing and non‐expressing epithelial regions and their corresponding stromal compartments. A total of 18,676 protein‐coding genes were assessed from prostatectomy samples collected from patients with localised prostate cancer. In the collected sample cohort, ANO7 exhibited a distinct, heterogeneous, on–off epithelial expression pattern, enabling an in‐depth analysis of ANO7‐dependent processes. ANO7‐positive epithelium was predominantly enriched with luminal epithelial cells and a specific NK cell subtype, CD56bright. In contrast, ANO7‐negative regions were characterised by enrichment of club cells, inflammation, and features of proliferative inflammatory atrophy. Gene‐set enrichment analysis revealed that ANO7 expression is associated with androgen receptor (AR) signalling and lipid metabolism. A detailed analysis of differentially expressed genes identified an ANO7‐ signature, which consisted of genes co‐expressed with ANO7 in luminal cells, that demonstrated high consistency in bulk RNA‐sequencing (RNA‐seq) data. The ANO7‐signature was enriched for AR‐regulated genes, which highlighted lipid metabolism processes, particularly arachidonic acid metabolism, as a key metabolic feature of the ANO7‐positive epithelium. Furthermore, the ANO7‐signature demonstrated clinical significance in low‐grade PrCa, correlating with a better response to therapy. In summary, these results highlight the potential role of ANO7 in regulating lipid metabolism associated with androgen signalling in benign luminal cells and low‐grade cancer, reinforcing the hypothesis that ANO7 functions as a tumour suppressor. © 2025 The Pathological Society of Great Britain and Ireland.
After 75 Years of Methotrexate, Can Treatment Results be Improved with Appropriate Folinic Acid Rescue?
Authors: Cohen Ian J. 
Published: February 2025

ABSTRACTAfter 75 years of clinical use of folic acid antagonists such as methotrexate, relevant pharmacological data currently important for the effective and safe use of methotrexate were reviewed to see if it is possible to improve outcomes. Specifically, to improve how high‐dose methotrexate (HD‐MTX) can be given safely, what doses of MTX (methotrexate) are adequate to achieve therapeutic levels, and what is the appropriate folinic acid (FA) dose for effective rescue. This review is based on 50 years of personal experience with the use of HD‐MTX in published literature. Many pharmacologic studies were performed over 50 years ago, but are still relevant and stand up to scrutiny today. What should be considered HD‐MTX and how it can be given safely and effectively without late toxicity are presented. The variables responsible for effective folinic acid rescue, especially the doses of MTX and folinic acid and the time to start of rescue, are discussed. Understanding these highlighted aspects of therapy could help to prevent acute toxicity, improve treatment results, and prevent late effects.
Benefits of Routine Screening for Parental Distress in Pediatric Cancer: A Pilot Implementation Effectiveness Study
Authors: Skrabal Ross Xiomara , Kemp Janine , Radford Claire , Carter Amanda , Davey Lara , Day Toni , Tyack Zephanie , Carter Hannah , Bradford Natalie 
Published: February 2025

ABSTRACTObjectiveTo explore the preliminary effectiveness and implementation outcomes (feasibility, acceptability, cost) of an intervention (eMaP), which provides electronic psychosocial screening and support for parents of children and adolescents with cancer (0–18 years).MethodsThis single‐site, pilot implementation‐effectiveness study, integrated routine screening into standard care for parents of children 12–16 weeks post diagnosis. Parents completed an online distress screening questionnaire, and those scoring high in distress received additional support from a social worker. Feasibility was measured using study uptake and completion rates, while costs were calculated based on social workers’ time. Qualitative interviews provided insights into acceptability.ResultsOut of 66 parents approached, 57 (86%) agreed to participate, indicating strong interest. Preliminary results showed that parents with concerning initial distress levels, who received clinical social worker support, experienced significant reductions in distress and support needs, and improvements in quality of life from baseline to T2 (8 weeks later) (all p ≤ 0.01). Routine monthly screening was considered feasible and valuable by both parents and social workers. Parents appreciated distress screening as a means of increasing awareness of their needs and valued the additional psychosocial support provided when indicated, while social workers found it useful for capturing clinical information often missed in standard care.ConclusionThe eMaP intervention was feasible and acceptable to both parents and staff, showing promise to improve parents’ psychosocial outcomes. The study suggests the need for refinements of the intervention and explorations of effectiveness in improving parents’ outcomes in future studies.
Social Support and School Belonging for Gender Diverse Students
Authors: Gilbertson Megan L. 
Published: February 2025

ABSTRACTTransgender and gender diverse (TGD) students often face discrimination, harassment, and inadequate support in educational settings, leading to increased stress and negative health outcomes. This study investigated differences in perceived social support from various sources and types between TGD students and their cisgender peers, and how this support is associated with school belonging. A total of 744 middle school students from a Midwestern US school participated in the survey, including 60 students who identified as TGD. Compared to cisgender students, TGD students reported lower levels of social support from all sources (teachers, peers, and parents) and types (emotional, appraisal, instrumental, and informational). Teacher support was positively associated with school belonging for TGD students, while parent support was negatively associated. Among TGD students, appraisal and informational support were positively associated with school belonging, while emotional and instrumental support showed no significant associations. For cisgender students, all types of social support were positively associated with school belonging. These findings highlight the unique challenges faced by TGD students in terms of social support and school belonging, and implications are discussed.
Communication Between Individuals With Borderline Personality Disorder and Their Partners
Authors: Lauzon Claire , Di Bartolomeo Alyssa A. , Varma Sonya , Boritz Tali , Liebman Rachel , Monson Candice , Fitzpatrick Skye 
Published: February 2025

ABSTRACTBorderline personality disorder (BPD) is characterized by frequent and intense conflict in intimate relationships. Emerging theoretical perspectives have suggested that communication from both individuals with BPD and their partners during times of conflict may exacerbate BPD pathology and therefore reflect an important target for intervention. Communication samples between individuals with BPD and their partners (N = 18 couples) who were seeking conjoint therapy were coded using the Rapid Marital Interactions Coding System‐2. No differences were found in the amount of positive, constructive or hostile communication between individuals with BPD and their partners, and both partners used significantly more constructive communication than positive or hostile, though they were misaligned in their use of constructive communication. These findings challenge the notion that individuals with BPD communicate in a more hostile or conflictual way than their partners, despite the stigma they face in healthcare and community settings.
Taming interleukin‐12: Engineering of bispecific antibody‐based IL‐12 mimetics with biased agonism capacities
Authors: Lipinski Britta , Unmuth Laura , Arras Paul , Endruszeit Ron , Becker Stefan , Rödel Jonathan Mathias , Scheller Jürgen , Pudewell Silke , Floss Doreen M. , Krah Simon , Harwardt Julia , Doerner Achim , Helming Laura , Xu Chunxiao , Menrad Andreas , Evers Andreas , Kolmar Harald , Elter Desislava , Pekar Lukas , Zielonka Stefan 
Published: February 2025

AbstractIn this work, we have generated bispecific interleukin (IL)‐12 surrogate agonists based on camelid‐derived single‐domain antibodies (sdAbs) targeting the IL‐12 receptor (IL‐12R) subunits IL‐12Rβ1 and IL‐12Rβ2. Following immunization and antibody display‐based paratope isolation, respective sdAbs were combinatorially reformatted into a monovalent bispecific architecture by grafting resulting paratopes onto the hinge region of a heterodimeric Fc region. Functional characterization using NK‐92 cells enabled the identification of multiple different sdAb‐based bispecifics displaying divergent IL‐12R agonism capacities as analyzed by STAT4 phosphorylation. Further investigations by harnessing peripheral blood mononuclear cells (PBMCs) from healthy donors revealed attenuated pSTAT4 activation compared to recombinant human (rh) wild‐type IL‐12 regarding both natural killer (NK)‐cell and T‐cell activation but robust IL‐12R agonism on stimulated T cells. While several sdAb‐based IL‐12 mimetics were nearly inactive on NK cells as well as T cells obtained from PBMCs, they elicited significant STAT4 phosphorylation and interferon (IFN)‐γ release on stimulated T cells as well as an IL‐12‐like transcriptional signature. Furthermore, we demonstrate that the activity of receptor agonism of generated bispecific IL‐12 mimetics can also be biased towards stimulated T cells by changing the spatial orientation of the individual sdAbs within the molecular design architecture. Taken together, we present an alternative strategy to generate IL‐12‐like biologics with tailor‐made characteristics.
Land‐surface interactions with the atmosphere over the Iberian Semi‐arid Environment (LIAISE): First mesoscale modelling intercomparison
Authors: Jiménez Maria A. , Cuxart Joan , Boone Aaron A. , Le Moigne Patrick , Lunel Tanguy , Mercader Jordi , Miró Josep R. , Best Martin , Brooke Jennifer K. 
Published: February 2025

AbstractLand‐surface–atmosphere interactions determine atmospheric boundary‐layer (ABL) features, and in the case of semi‐arid regions the water availability in the root‐zone layer is a major factor. To explore this issue, the Land‐surface Interactions with the Atmosphere over the Iberian Semi‐arid Environment (LIAISE) initiative organized an observational campaign in the Eastern Ebro River sub‐basin in summer 2021, focusing on the effect of surface heterogeneities on the ABL in a semi‐arid environment enclosing a large irrigated area. With the aim of understanding the performance of numerical models for the region studied, a mesoscale modelling intercomparison was undertaken for a three‐day case study in July 2016. The intercomparison consisted of three models, Meso‐NH, the Unified Model (UM), and Weather Research and Forecasting (WRF), run with two embedded domains, with corresponding horizontal resolutions of 2 km and 400 m and similar vertical grids. This is a case dominated by well‐developed thermally driven circulations in the Ebro Basin, allowing an investigation of the representation of surface features in the models and its impact on the organization of the flow at lower levels. Other effects are also explored, such as using different initial and lateral boundary conditions, changing horizontal resolution, or modifying some surface features. The mesoscale circulations generated by the three models are similar and accurate when compared with the surface observations, variations being found at the ABL scales related to the representation of surface processes in each model, which provide different grid values of the surface fluxes. It is found that the models do not perform as well over the irrigated areas as in the rest of the domain. The challenge at this point is to relate the model biases to the particularities of the parameterizations and physiographic databases used by each model.
Maintenance Modeling for a Multi‐Component System Considering Dynamic Importance of Components and Imperfect Repair
Authors: Qi Faqun , Kong Jiahui , Li Mi , Zhang Chengjie , Cui Mingguang , Xu Yun 
Published: February 2025

ABSTRACTA system consisting of a critical and an auxiliary component is studied, and imperfect maintenance models considering degradation interaction and maintenance effect are developed. The auxiliary component assists the critical one in completing the system function, and its failure does not directly lead to the system failure. When the critical component deteriorates to the preventive maintenance (PM) threshold, it is restored by PM. When the auxiliary component deteriorates to a poor condition, significantly affecting the system reliability, the dynamic importance of the auxiliary component is considered, and the maintenance decision is provided according to the state of the auxiliary component. By minimizing the system's long‐term average cost, the optimal PM threshold for the critical component and maintenance threshold for the auxiliary component are derived. Finally, the effectiveness of the proposed model is further demonstrated with examples, comparison with previous model and comprehensive sensitivity analysis.
Remediation of Inorganic Mercury From Spent Fluorescent Lamps Using Heterotrophic Bacterial Strains Isolated From Domestic Wastewater
Authors: Gohari Samaneh , Aali Rahim , Alidadi Hosein , Askarizadeh Fatemeh , Esmaeili Zahra , Farsiani Hadi 
Published: February 2025

ABSTRACTInorganic mercury from spent fluorescent lamps is classified as hazardous waste and has become a severe concern for the environment. This study aimed to survey the effect of indigenous heterotrophic bacteria from domestic wastewater on the remediation of inorganic mercury. In this study, the mercury from phosphor power was leached and then detoxified using bacterial strains isolated from domestic wastewater. Nitric and hydrochloric acid solutions were applied for mercury leaching. Twenty‐eight bacterial strains were isolated from domestic wastewater on Reasoner's 2A agar. Then, to check the bacterial mercury resistance, these strains were sub‐cultured on a Luria‐Bertani medium amended with mercury chloride. 16S rRNA gene sequencing was used to determine the genus and species of mercury‐reducing bacteria. This method was performed by the polymerase chain reaction (PCR) amplification of 16S rRNA, followed by sequencing. Bacterial strains demonstrating the remediation capacity of mercury were Acinetobacter junii, Aeromonas cariae, Pseudomonas alcaliphila, Pseudomonas marginalis, Aeromonas sanarellii, Klebsiella singaporensis, and Pseudomonas simiae. These strains showed bio‐uptake efficiency within the range of 67.9% to 92.2%. The lowest and highest rates of mercury remediation were related to P. simiae (67.9%) and P. marginalis (92.24%), respectively. The results show that 18 of these 28 bacterial strains displayed resistance to mercury. To determine the bio‐uptake capacity, the bacteria isolated from wastewater were exposed to the mercury leached from the fluorescent lamps, and mercury content was measured by atomic absorption spectrophotometry. The seven bacteria isolated from wastewater reduced the mercury concentration to less than 5 μg/L. The results of this study revealed that sewage heterotrophic bacteria can be used as an efficient and low‐cost method for mercury bioremediation in discarded fluorescent lamps.
Active Disturbance Rejection Control for the Stabilization of a Linear Hyperbolic System
Authors: Balogoun Ismaïla , Marx Swann , Orlov Yury , Plestan Franck 
Published: February 2025

ABSTRACTThis paper deals with the stabilization of a linear hyperbolic system subject to a boundary disturbance. Our feedback design relies on the strategy called active disturbance rejection control (ADRC). The unknown disturbance is estimated by an extended state observer. We prove the existence of solutions of the closed‐loop system and the global asymptotic stability of the closed‐loop system. A numerical example is given to illustrate the efficiency of our strategy.
Input‐To‐State Stability of Switched Systems Under the New Mode‐Dependent Dynamic Event‐Triggered Impulsive Control
Authors: Gao Lijun , Li Mengjie , Yang Suo 
Published: February 2025

ABSTRACTThe aim of this article is to study non‐linear switched systems and obtain conditions of input‐to‐state stability (ISS) via dynamic event‐triggered impulsive control (DETIC) scheme, where the impulse instants are produced by a novel mode‐dependent dynamic event‐triggered impulsive mechanism (MD‐DETIM). The switching instants may not be synchronized with the event triggering instants. Different from conventional static event‐triggered impulsive control (ETIC), the MD‐DETIM can greatly decrease the number of data updates and significantly save communication transmission resource. Based on the mode‐dependent dynamic event‐triggered impulsive control (MD‐DETIC) scheme and the admissible edge‐dependent average dwell time switching rule, Lyapunov‐based results are created, which can effectively guarantee ISS of non‐linear switched systems. Meanwhile, a positive minimum bound is needed to obtain for triggering interval between any two adjacent triggering instants to exclude Zeno behavior. Eventually, two examples are provided to explicate the validity and less conservativeness of conclusions in this paper.
Stimuli‐responsive molecularly imprinted materials: Fundamentals and applications
Authors: Sullivan Mark V. , Lasserre Perrine , Blackburn Chester , Turner Nicholas W. , Sellergren Börje 
Published: February 2025

AbstractStimuli‐responsive molecularly imprinted polymers (MIPs) are exciting smart materials that are gaining substantial interest within the research community due to their versatility and possible widespread applications in biosensing, biomedicine and diagnostics, as well as chromatography and separation sciences. These materials offer significant advantages as recognition materials over their biological counterparts (antibodies) because of their ease and low cost of production along with their robustness and resistance to the extremes of temperature and pH. This much needed review aims to provide an updated summary of the various stimuli‐responsive MIPs reported to date including those relying on thermo, pH, photo, biomolecule, ion, magnetic and electrical stimuli and includes their design and synthesis. The review also explores the potential applications of the stimuli‐responsive MIPs, particularly in the fields of biosensors and diagnostics, along with biological imaging, drug delivery, disease treatments and interventions and the separation of targets from complex media. The advantages and disadvantages of the current stimuli‐responsive MIPs set out in the review, allows for researchers to gather a concise understanding of these smart‐materials and should pave the way for new methods of development and real‐world applications. We believe the review is a helpful and necessary guide for the future evolution and application of stimuli‐responsive MIPs.
Understanding the Limited Uptake of Reprocessed Construction Materials: A Pro‐Environmental Behaviour Perspective
Authors: Tennakoon Gihan Anuradha , Rameezdeen Raufdeen , Chileshe Nicholas 
Published: February 2025

ABSTRACTReverse logistics (RL) aims to direct demolition waste (DW) away from landfills towards alternative uses by converting it into reprocessed construction materials (RCMs). However, recent studies indicate that the uptake of RCMs remains limited, adversely affecting the effectiveness of RL for DW. Therefore, developing markets for these materials is crucial. Consequently, this study aimed to identify factors limiting the uptake of RCMs so that effective strategies can be designed to address such limitations. The study adopted a qualitative survey approach, with interviews conducted with construction professionals involved in material selection. Findings regarding the limiting factors were analysed through the lens of the Attitude–Behaviour–Context (ABC) theory, which is rooted in the domain of pro‐environmental behaviour. Consistent with the ABC theory, the findings revealed that a combination of personal and contextual factors limits the uptake of RCMs. Addressing these factors is essential for driving market uptake of RCMs and improving RL outcomes.