bluuebunny/crossref_metadata_2025_split huggingface dataset:
Probe-integrated electrochemical immunosensor based on electrostatic nanocage array for reagentless and sensitive detection of tumor biomarker
Authors: Chen Dong , Luo Xuan , Xi Fengna 
Published: March 2023

Sensitive detection of tumor biomarkers is crucial for early diagnosis and prognosis evaluation of cancer. Owing to no need of labelled antibody, formation of sandwich immunocomplexes and additional solution-based probe, probe-integrated electrochemical immunosensor for reagentless detection of tumor biomarkers is highly desirable. In this work, sensitive and reagentless detection of a tumor biomarker is realized based on fabrication of a probe-integrated immunosensor by confining redox probe in electrostatic nanocage array modified electrode. Indium tin oxide (ITO) electrode is employed as the supporting electrode because it is cheap and easily available. The silica nanochannel array consisted of two layers with opposite charges or different pore diameters was designated as bipolar films (bp-SNA). In this work, Electrostatic nanocage array is equipped on ITO electrode by growth of bp-SNA with two layered nanochannel array having different charge properties including a negatively charged silica nanochannel array (n-SNA) and a positively charged amino-modified SNA (p-SNA). Each SNA can be easily grown with 15 s using electrochemical assisted self-assembly method (EASA). Methylene blue (MB) is applied as the model electrochemical probe with positive charge to be confined in electrostatic nanocage array with stirring. The combination of the electrostatic attraction from n-SNA and the electrostatic repulsion from p-SNA endows MB with highly stable electrochemical signal during continuous scanning. When the amino groups of p-SNA are modified using the bifunctional glutaraldehyde (GA) to introduce aldehydes, the recognitive antibody (Ab) of the most commonly used tumor biomarker, carcinoembryonic antigen (CEA), can be covalently immobilized. After the non-specific sites are blocked, the immunosensor is successfully fabricated. As the formation of antigen-antibody complex decreases electrochemical signal, the immunosensor can achieve reagentless detection of CEA ranged from 10 pg/mL to 100 ng/mL with a low limit of detection (LOD, 4 pg/mL). Determination of CEA in human serum samples is realized with high accuracy.
Combined analysis of plasma metabolome and intestinal microbiome sequencing to explore jiashen prescription and its potential role in changing intestine–heart axis and effect on chronic heart failure
Authors: Cui Xialian , Su Yangyan , Huang Xiaotong , Chen Jiaping , Ma Jiang , Liao Peiran , He Xin 
Published: March 2023

BackgroundHeart failure (HF) is a syndrome with global clinical and socioeconomic burden worldwide owing to its poor prognosis. Jiashen Prescription (JSP), a traditional Chinese medicine (TCM) formula, exhibits unambiguous effects on treating HF. Previously, we have reported that underlying mechanisms of JSP by an untargeted metabolomics approach, but the contribution of gut microbiota and metabolic interaction to the cardioprotective efficacy of JSP remains to be elucidated.Materials and methodsFirstly, the rat model of heart failure was established by the permanent ligation of the left anterior descending coronary artery. The efficacy evaluation of JSP in treating HF rats was per-formed by left ventricular ejection fraction (LVEF). Then, 16S rRNA gene sequencing and LC/MS-based metabolomic analysis were utilized to explore the characteristics of cecal-contents microecology and plasma metabolic profile, respectively. After that, the correlation between intestinal micro-ecological characteristics and plasma metabolic characteristics was analyzed to explore the potential mechanism of the JSP treatment in HF.ResultsJSP could improve the cardiac function of heart failure rats and thus ameliorate heart failure via enhancing rat LVEF. Results of intestinal flora analysis revealed that JSP not only adjusted gut microbiota disturbances by enriching species diversity, reducing the abundance of pathogenic bacteria (such as Allobaculum, Brevinema), as well as increasing the abundance of beneficial bacteria (such as Lactobacillus, Lachnospiraceae_NK4A136_group), but also improved metabolic disorders by reversing metabolite plasma levels to normality. Through the conjoint analysis of 8 metabolites and the OTUs relative abundance data in the 16srRNA sequencing results by WGCNA method, 215 floras significantly related to the eight compounds were identified. The results of the correlation analysis demonstrated a significant association between intestinal microbiota and plasma metabolic profile, especially the significant correlation of Ruminococcaceae_UCG-014 and Protoporphyrin IX, Ruminococcaceae_UCG-005, Christensenellaceae_R-7_group and nicotinamide, dihydrofolic acid.ConclusionThe present study illustrated the underlying mechanism of JSP to treat heart failure by affecting intestinal flora and plasma metabolites, provide a potential therapeutic strategy against heart failure.
Experimental research on the water precipitation performance of polymer colloid and fly ash colloid and their applications as fire extinguishing materials in mines
Authors: Shang Yuyang 
Published: March 2023

In this paper, polymer colloid and fly ash colloid fire extinguishing materials are selected. Through experimental preparation and water precipitation characteristics research, colloid fire extinguishing materials and optimal concentration ratio suitable for Binchang mining area are selected to effectively control the fire area. The results show that, the higher the concentration of polymer colloidal powder mixed with water, the shorter the gelling time. After the fly ash is mixed with the gelling agent and water, when the concentration ratio of fly ash to water remains the same, the greater the concentration of the gelling agent, the shorter the gelling time. After the colloid is left standing for 24 h, the smaller the concentration is, the more water is precipitated, and the higher the water precipitation rate is. Through comparison, it was found that when the polymer colloid was selected as the fire extinguishing material, with the concentration ratio of polymer colloid material to water being 3.5%–4%: 96.5%–96%, effective control could be achieved over the fire zones in the goaf of Binchang mine. The research is of great reference value for the control of mine fires.
Analysis of the generation potential of hybrid solar power plants
Authors: Martins Gleyzer , Mendoza Oscar Saul Hernandez , Amjad Muhammad , Bandarra Filho Enio Pedone 
Published: March 2023

Photovoltaic solar energy has emerged in Brazil in distributed generation due to affordable costs and its application in different segments of the market. However, the electric energy storage in the photovoltaic systems is only viable for certain conditions in which the installation does not have access to the electricity network and the thermal portion of the solar energy is lost to the ambient. Heliothermic power plant represents another system available for solar electricity generation, which has higher costs, but takes better advantage of the portion of thermal energy and allows energy storage efficiently to meet the demands during periods without incident solar radiation. Heliothermic and photovoltaic generation have great potential in the Southeast and Northeast regions of Brazil, but the costs of both technologies cannot meet the demands during periods without solar radiation. This work presents a conceptual analysis of compound parabolic solar concentrators along with hybrid of photovoltaic and thermal collector systems to meet both the demand during sunlight hours and without solar radiation. The investigation consisted of implementing an organic Rankine cycle with different heliothermic plant configurations and the analysis of the diode model to examine different commercial photovoltaic cells operated in the Rankine cycle evaporation region. The results showed that the best working fluid for the application is R245fa with Urea-NaCl as molten salt for the thermal energy storage. The expected electrical power for the hydride cycle is 1580W at 2600 rpm and at 80°C of evaporation temperature, enabling a power generation for 1 hour and 20 min without solar radiation.
Comparative study on the performance of the application of clean alternative fuels in SOFC/ICE hybrid power systems on electric aircraft
Authors: Li Chengjie , Wang Zixuan , Liu He , Qin Jiang , Wei Liqiu 
Published: March 2023

Solid oxide fuel cells (SOFC) have gradually become possible to be used in the aviation field because of their high efficiency and fuel flexibility. The number of internal combustion engines (ICE) that are already in use is large, which makes it difficult to completely abandon them in a short period. Hence, finding low-carbon alternative fuels is significant for the development of cleaner internal combustion engines. In this paper, different hybrid power systems configured with different fuels, i.e., hydrogen, methanol, liquid ammonia, isooctane, natural gas and gasoline, which consist of a solid oxide fuel cell and an internal combustion engine, are constructed to help find the optimal set of fuel and system structure by analyzing the power generation performance and weight of different systems. The results show that the hybrid power system with ammonia or methanol (except gasoline) as fuel presents the lowest actual fuel consumption rate (around 310 g/kWh). The hybrid power system with ammonia as fuel performs the highest power generation efficiency of 61.58%, and the corresponding power density is estimated at 0.556 kW/kg. In conclusion, the proposed system with ammonia as fuel has obvious advantages in fuel consumption rate, power generation efficiency, carbon emission reduction and fuel storage.
Pile covering of waste disposal using technosols made of municipal solid waste incineration bottom ash and coal combustion residues: A column leaching experiment
Authors: Bilibio Carolina , Hensel Oliver 
Published: March 2023

Technosols are characterized by a substantial quantity of artifacts or industrial wastes in the upper 100 cm. They can be used as cover caps in mining rehabilitation, in which vegetation cover is subsequently established. However, prolific vegetation growth can be limited by the chemical and physical properties of technogenic substrates. As further studies need to be conducted to evaluate the suitability of municipal solid waste incineration bottom ash and coal combustion residues as cover caps, the pH, electrical conductivity, total porosity, and bulk density of these substrates were measured. Furthermore, the seepage pH and electrical conductivity of the covered and uncovered technosols after saturation were determined. The municipal solid waste incineration bottom ash, 4–10 mm in diameter, showed the highest bulk density (1.16 g cm-3) and lowest total porosity by saturation (24.7%). In contrast, the coal combustion residues registered the highest total porosity (57.2%). The coal combustion residues also revealed a higher pH of 12.5 and electrical conductivity values of 7.8 mS cm-1. Furthermore, no differences were observed between the treatments for pH seepage (8.2–8.3) and its electrical conductivity (14.3–16.0 mS cm-1) in the covered and uncovered columns, when using a technosol. This study provides information on the use of municipal solid waste incineration bottom ash and coal combustion residues as evapotranspiration covers during mining rehabilitation. The management of municipal solid waste residues is a global challenge and the use of this resource is valuable not only for the mining industry but potentially also in other fields, such as construction. The heavy metal content of the bottom ash from municipal solid waste incineration and coal combustion residues should be studied before implementing these waste residues on a large scale.
High serum levels of the C-propetide of type V collagen (PRO-C5) are prognostic for short overall survival in patients with pancreatic ductal adenocarcinoma
Authors: Nissen Neel I. , Johansen Astrid Z. , Chen Inna M. , Jensen Christina , Madsen Emilie A. , Hansen Carsten P. , Thorlacius-Ussing Jeppe , Karsdal Morten , Johansen Julia S. , Diab Hadi M. H. , Jørgensen Lars N. , Willumsen Nicholas 
Published: March 2023

Introduction: Pancreatic ductal adenocarcinoma (PDAC) is characterized by a pronounced fibrotic tumor microenvironment, which impairs treatment response. Type I and V collagens are responsible for the densely packed fibrils in the tumor fibrosis environment. While the role of the major type I collagen in cancer is well described, less is known about the minor type V collagen. Quantifying collagen propeptides in serum has been shown to have prognostic and predictive value. In this study, we evaluated the clinical utility of measuring the propeptide of type V collagen (PRO-C5) in serum from a discovery cohort and a validation cohort of patients with PDAC as well as in non-pancreatic solid tumor types to explore the relevance of the PRO-C5 biomarker in cancer.Methods: Serum PRO-C5 was measured in three cohorts: a discovery cohort (19 healthy controls, 12 patients with chronic pancreatitis and 33 patients with PDAC (stage I-IV)), a validation cohort (800 patients with PDAC (stage I-IV)), and a non-pancreatic solid tumor type cohort of 33 healthy controls and 200 patients with 10 different non-pancreatic solid tumor types. The levels of serum PRO-C5 in patients with cancer were compared to levels in healthy controls. The association between PRO-C5 levels and overall survival (OS) was evaluated in patients with PDAC after adjusting for established prognostic factors.Results: PRO-C5 was significantly increased in serum from patients with PDAC compared to healthy controls (p &lt; 0.001). High PRO-C5 levels were significantly associated with short OS in both the discovery- and the validation cohort, especially in early stages of PDAC (validation cohort stage II, HR = 2.0, 95%CI1.2-3.4). The association was independent of other prognostic parameters including stage, performance status and CA19-9. Furthermore, serum levels of PRO-C5 were significantly increased in serum from patients with other non-pancreatic solid tumor types compared to healthy controls.Conclusion: High levels of serum PRO-C5 is prognostic for short OS in patients with PDAC and may provide clinical value in many other tumor types beyond PDAC. This underlines the importance of type V collagen in tumor fibrosis. PRO-C5 could have the potential to be used in several aspects within drug discovery, patient stratification and drug efficacy.
Clinical and genomic characterization of Chinese patients with functional high-risk multiple myeloma: A real-world validation study
Authors: Wang Yu-tong , Chu Bin , Zhou Tian-guan , Lu Min-qiu , Shi Lei , Gao Shan , Fang Li-juan , Xiang Qiu-qing , Zhao Xin- , Wang Meng-zhen , Sun Kai , Bao Li 
Published: March 2023

ObjectivePrecise risk stratification is increasingly essential in the management of multiple myeloma (MM) as some standard-risk (SR) patients still exhibit similar poor outcomes as genetically high-risk (GHR) patients in the era of novel agents. It has recently been demonstrated that functional high-risk (FHR) patients, those with suboptimal response to first-line induction therapy or early relapse within 12 months, have identifiable molecular characteristics from the SR group in the CoMMpass dataset. However, these findings lack practical validation in the real world.MethodsMM cells purified by CD138 microbeads from newly diagnosed MM (NDMM) patients received fluorescence in situ hybridization and sequencing with a 92-gene Panel. Cytogenetic abnormalities defined GHR patients with t(4;14) or t(14;16) or complete loss of functional P53 or 1q21 gain and International Staging System (ISS) stage 3. SR group was patients who did not fulfill any criteria for GHR or FHR.ResultsThere were 145 patients with NDMM, 78 in the SR group, 56 in the GHR group, and 11 in the FHR group. In the FHR group, eight patients were suboptimal responses to induction therapy, and three relapsed within 12 months. We found that male patients, patients with extra-medullary plasmacytoma (EMD), circulating clonal plasma cells (CPC) ≥0.05%, and P53 mono-allelic inactivation were significantly higher in the FHR group compared to the SR group. After a median follow-up of 21.0 months, the median progression-free survival (PFS) and overall survival (OS) were 5.0 months, 19.1 months and 36.6 months in the FHR, GHR, and SR groups, respectively. Compared to the SR group, FHR patients had a higher frequency of mutations in MKI67, ERN1, and EML4. GO analysis showed that mutations in FHR were enriched for oxidative stress, chromosomal segregation, and hypoxia tolerance.ConclusionThe FHR found in the SR NDMM patient group has unique clinical features, including being male, with EMD and CPC, and genetic characteristics of mutations affecting oxidative stress, chromosome segregation, and hypoxia tolerance. In contrast to previous reports, our data suggested that patients with P53 mono-allelic inactivation should be classified in the GHR group rather than the FHR group.
Relationship between air pollution exposure and the progression of idiopathic pulmonary fibrosis in Madrid: Chronic respiratory failure, hospitalizations, and mortality. A retrospective study
Authors: Mariscal-Aguilar Pablo , Gómez-Carrera Luis , Carpio Carlos , Zamarrón Ester , Bonilla Gema , Fernández-Velilla María , Torres Isabel , Esteban Isabel , Regojo Rita , Díaz-Almirón Mariana , Gayá Francisco , Villamañán Elena , Prados Concepción , Álvarez-Sala Rodolfo 
Published: March 2023

IntroductionAir pollution has a significant impact on the morbidity and mortality of various respiratory diseases. However, this has not been widely studied in diffuse interstitial lung diseases, specifically in idiopathic pulmonary fibrosis.ObjectiveIn this study we aimed to assess the relationship between four major air pollutants individually [carbon monoxide (CO), nitrogen dioxide (NO2), ozone (O3), and nitrogen oxides (NOx)] and the development of chronic respiratory failure, hospitalization due to respiratory causes and mortality in patients with idiopathic pulmonary fibrosis.MethodsWe conducted an exploratory retrospective panel study from 2011 to 2020 in 69 patients with idiopathic pulmonary fibrosis from the pulmonary medicine department of a tertiary hospital. Based on their geocoded residential address, levels of each pollutant were estimated 1, 3, 6, 12, and 36 months prior to each event (chronic respiratory failure, hospital admission and mortality). Data was collected from the air quality monitoring stations of the Community of Madrid located &lt;3.5 km (2.2 miles) from each patient's home.ResultsThe increase in average values of CO [OR 1.62 (1.11–2.36) and OR 1.84 (1.1–3.06)], NO2 [OR 1.64 (1.01–2.66)], and NOx [OR 1.11 (1–1.23) and OR 1.19 (1.03–1.38)] were significantly associated with the probability of developing chronic respiratory failure in different periods. In addition, the averages of NO2, O3, and NOx were significantly associated with the probability of hospital admissions due to respiratory causes and mortality in these patients.ConclusionAir pollution is associated with an increase in the probability of developing chronic respiratory failure, hospitalization due to respiratory causes and mortality in patients with idiopathic pulmonary fibrosis.
Estimation of subjective quality of life in schizophrenic patients using speech features
Authors: Shibata Yuko , Victorino John Noel , Natsuyama Tomoya , Okamoto Naomichi , Yoshimura Reiji , Shibata Tomohiro 
Published: March 2023

IntroductionPatients with schizophrenia experience the most prolonged hospital stay in Japan. Also, the high re-hospitalization rate affects their quality of life (QoL). Despite being an effective predictor of treatment, QoL has not been widely utilized due to time constraints and lack of interest. As such, this study aimed to estimate the schizophrenic patients' subjective quality of life using speech features. Specifically, this study uses speech from patients with schizophrenia to estimate the subscale scores, which measure the subjective QoL of the patients. The objectives were to (1) estimate the subscale scores from different patients or cross-sectional measurements, and 2) estimate the subscale scores from the same patient in different periods or longitudinal measurements.MethodsA conversational agent was built to record the responses of 18 schizophrenic patients on the Japanese Schizophrenia Quality of Life Scale (JSQLS) with three subscales: “Psychosocial,” “Motivation and Energy,” and “Symptoms and Side-effects.” These three subscales were used as objective variables. On the other hand, the speech features during measurement (Chromagram, Mel spectrogram, Mel-Frequency Cepstrum Coefficient) were used as explanatory variables. For the first objective, a trained model estimated the subscale scores for the 18 subjects using the Nested Cross-validation (CV) method. For the second objective, six of the 18 subjects were measured twice. Then, another trained model estimated the subscale scores for the second time using the 18 subjects' data as training data. Ten different machine learning algorithms were used in this study, and the errors of the learned models were compared.Results and DiscussionThe results showed that the mean RMSE of the cross-sectional measurement was 13.433, with k-Nearest Neighbors as the best model. Meanwhile, the mean RMSE of the longitudinal measurement was 13.301, using Random Forest as the best. RMSE of less than 10 suggests that the estimated subscale scores using speech features were close to the actual JSQLS subscale scores. Ten out of 18 subjects were estimated with an RMSE of less than 10 for cross-sectional measurement. Meanwhile, five out of six had the same observation for longitudinal measurement. Future studies using a larger number of subjects and the development of more personalized models based on longitudinal measurements are needed to apply the results to telemedicine for continuous monitoring of QoL.
The safety and efficiency of benzoyl peroxide for reducing Cutibacterium acnes in the shoulder: An updated systematic review and meta-analysis
Authors: Fan DingYuan , Ma Jia , Liu XiaoHua , Zhang Sheng , Sun Jin , Li Yan , Jiang Bo , Zhang Lei 
Published: March 2023

BackgroundCutibacterium acnes (C. acnes), a common pathogen, contributes significantly to infections in shoulder surgery. Prevention of shoulder infection is crucial to improve postoperative functional recovery and reduce costs. This study aimed to perform a systematic review and meta-analysis to assess the safety and efficacy of 5% benzoyl peroxide (BPO) application in the shoulder to decrease C. acnes.MethodsThree electronic databases were searched as follows: PubMed, Embase, and the Cochrane Library databases. Data extraction for this study was performed by two independent reviewers, and only level I and level II studies were included. The outcome data sources of individual studies were pooled. The fixed-effect model was used to determine the meta-analysis.ResultsThere were five level I studies and five level II studies. The results showed that the 5% BPO group had a lower risk of C. acnes positivity [OR, 0.21 (0.15, 0.30), I2 = 24, p &lt; 0.00001]. The pooled analysis results showed that there was no significant difference in the ability of 5% BPO and 5% BPO + clindamycin to reduce C. acnes. However, the lower rate of adverse events was significantly in favour of the non-BPO group compared with the 5% BPO group.ConclusionBPO can decrease C. acnes in the shoulder to prevent infection. However, the combination of BPO and clindamycin does not enhance this effect further.Level of evidenceII, Systematic review and meta-analysis.