bluuebunny/crossref_metadata_2025_split huggingface dataset:
Perancangan Film Pendek “ LEVEL UP “ sebagai kampanye Pola Hidup Sehat di Lingkungan E - Sport
Authors: Yahya Abdul Ghaffar Liwauddin Maghribi, Saiful Yahya 
Published: January 2025

E-sport, merupakan salah satu olahraga elektronik yang menggunakan permainan sebagai bidang kompetitif. Banyak kasus yang disebabkan oleh dampak negatif e-sport karena penggunaan yang berlebihan. Kampanye merupakan suatu proses kegiatan komunikasi individu atau kelompok yang dilakukan secara terlembaga dan bertujuan untuk menciptakan suatu efek atau dampak tertentu. Kampanye hidup sehat kepada pemain e-sport dibutuhkan untuk menumbuhkembangkan perilaku sehat pada pemain e-sport, mengingat aktifitas pemain e-sport yang kurang menggunakan anggota tubuh untuk berolahraga. Peneliti mengembangkan bentuk kampanye melalui pembuatan film yang mengangkat cerita kehidupan pemain e-sport. Metode analisa yang akan digunakan adalah analisa deskriptif kualitatif. Melalui metode analisa Deskriptif , Peneliti dapat mendapatkan pemahaman yang lebih jelas mengenai kejadian ,fenomena atau keadaan sosial yang terjadi di sekitar. Film pendek “Level Up” merupakan hasil pengembangan melalui pendekatan design thinking guna mengkampanyekan gaya hidup sehat di kalangan pemain e-sport. Design thinking terdiri tahapan empathise, define, ideate, prototipe dan test. Empathise digunakan untuk mendapatkan data awal yang berasal dari pemain e-sport, tahapan define digunakan untuk mendeskripsikan permasalahan yang dihadapi pemain e-sport. Tahapan ideate digunakan untuk menentukan ide-ide yang akan digunakan dalam pengembangan film, tahapan prototipe merupakan tahapan pembuatan film pendek “Level-Up” serta tahapan test digunakan untuk menguji film tersebut. Berdasarkan hasil uji coba kepada target audiens, diperoleh hasil total presentase 86% dimana menunjukan bahwa film pendek yang dirancang berhasil untuk dipublikasikan sebagai media informasi mengkampanyekan dampak negatif e-sport.
Variations In Methods For Storing Samples From Diabetes Mellitus Patients On The Results Of Urine Leukocyte Examination
Authors: Maulidiyah Hikmatul , Puspitasari  
Published: January 2025

Diabetes mellitus (DM) is often accompanied by both microvascular and macrovascular. One of the common complications in patients with diabetes mellitus is a urinary tract infection. The purpose of this study was to determine the difference between using a 10% formaldehyde preservative and storage at 4°C to delay urine leukocyte examination in diabetic patients. This study employed an analytical observationalmethod using urine samples from patients with diabetes mellitus. A total of six samples were obtained from Siti Fatimah Tulangan 'Aisyiyah Hospital. The research was conducted from May to June 2023. The results indicate that there are differences in the results of urine leukocyte examinations in diabetes mellitus patients based on the variations in sample storage methods.

Keywords: Delayed, diabetes mellitus, leukocytes, storage, urine.
Project-Based Learning Model with Blended Learning in Academic Writing: A Need Analysis
Authors: Ilham Ilham , Irwandi Irwandi , Ayun Zakiyah Qurata , Wida Saniyatil 
Published: January 2025

Conducting a need analysis is crucial for tailoring academic writing instruction to meet the students’ need. Need analysis serve as systematic tool for developing relevant learning materials that align with students’ learning needs. This research hold significant importance in adressing the integration of project-based learning (PjBL) and blended learning within the context of academic writing. By identifying the needs of students, it provides valuable insight for culliculum designers and educators in English education program. The research aims to identify the students' need for project-based learning with blended learning in academic writing. This study is conducted as survey research at the English education study program at Muhammadiyah University of Mataram and Mandalika University of Education. The questionnaire of collecting data consists of 61 statements with 11 factors. The result of the research shows that all of the students need eleven factors such as learning objectives, topic needs, teaching material requirements, needs assessment, the need of PJBL syntax, blended learning, social system need, requirement principle of reaction, need of support system, learning impact need, and need for impact accompanying learning. The results of this research are expected contributing to the lecturers, stakeholders, and curriculum development of project-based blended learning in academic writiting at the English Education department.
Hydroxyurea mitigates diabetic kidney disease through mTOR-S6K signaling pathway in STZ-induced diabetic mice
Authors: Cheng Wanying , Wang Cenzhu , Ma Meican , Zhou Yu 
Published: January 2025

BackgroundDiabetic kidney disease (DKD) is the leading risk factor for end-stage renal disease (ESRD). Hydroxyurea (HU), a sickle cell disease (SCD) drug approved by FDA, shows protective effect in nephropathy. This study aims to understand whether the application of HU could be effective to treat DKD.MethodsThe streptozotocin (STZ)-induced diabetic mice, and high glucose (HG)-treated human renal mesangial cells (HRMCs) were used to investigate the effect of HU on DKD. Serum creatinine and blood urea nitrogen levels reflecting renal function were evaluated. Histology was used to evaluate pathological changes. Indicators of inflammation and apoptosis were detected. Lastly, the mTOR-S6K pathway was explored by detecting the protein expression of S6K and phosphorylated S6K.ResultsIn STZ-induced diabetic mice, administration of HU (20 mg/kg) in drinking water for 16 weeks resulted in significant reductions in creatinine and urea nitrogen levels, alongside mitigating histopathological damage. Additionally, HU effectively suppressed the inflammatory response and apoptosis within the kidneys. HRMC cells were cultivated in HG conditions, and HU effectively attenuated the HG-induced inflammation and apoptosis. Moreover, HU treatment significantly inhibited the mTOR signaling pathway in both in both in vivo and in vitro experiments.ConclusionThis study unveils a new role of HU in alleviating diabetic kidney disease by modulating inflammation and apoptosis through the mTOR-S6K pathway. However, since HU did not significantly affect blood glucose levels, its therapeutic potential may be best realized when used in combination with standard antidiabetic therapies. Such a combination approach could simultaneously address hyperglycemia and renal dysfunction, offering a more comprehensive management strategy for DKD.
Salivary miRNAs and cytokines associated with diagnosis and prognosis of oral squamous cell carcinoma
Authors: Qin Yuxiao , Dong Xiaodan , Li Bo 
Published: January 2025

Oral squamous cell carcinoma (OSCC) is the most common malignant tumour in the oral and maxillofacial region. Early diagnosis can significantly improve the 5-year survival rate of patients with OSCC. Therefore, it is extremely important to differentiate OSCC patients early, easily and quickly. Human saliva contains a variety of components that can be used as biomarkers for the diagnosis and prognosis of OSCC. Studies have shown that salivary microRNAs (miRNAs) and cytokines are closely associated with the progression of OSCC. The aim of this review is to summarize the research progress of salivary biomarkers (miRNAs and cytokines) in the past 3 years, and to explore the possibility of using miRNAs and cytokines to improve the diagnosis and prognosis of OSCC.
Prognostic value of inflammatory markers for all-cause mortality in patients with acute myocardial infarction in the coronary care unit: a retrospective study based on MIMIC-IV database
Authors: Cao Fen , Jiang Jun-jun , Zhang Gui , Liu Jun , Xiao Ping , Tian Yang , Zhang Wei , Zhang Sheng , Hou Feng , Bao Zhong-Wu , Wu Kun , Zhu Yong-zhi 
Published: January 2025

BackgroundAcute myocardial infarction (AMI) is prevalent and perilous, leading to mortality and disability in the coronary care unit (CCU). This paper was to verify the correlation of neutrophil-to-lymphocyte ratio (NLR), systemic immune-inflammation index (SII), platelet-to-lymphocyte ratio (PLR), and systemic inflammation response index (SIRI) with all-cause mortality for AMI patients in the CCU.MethodsAdult patients diagnosed with AMI and admitted to CCU were selected from the MIMIC-IV database. Various clinical and laboratory data were extracted. Logistic regression models were employed to determine the correlation between NLR and in-hospital mortality, 30-day mortality, and 90-day mortality. Confounding factors were adjusted to validate the result robustness. Restricted cubic spline (RCS) curves were adopted to analyze the potential correlation between NLR and all-cause mortality. Meanwhile, the area under the receiver operating characteristic (ROC) curve (AUC) was utilized to compare the prediction ability of NLR, SII, PLR, and SIRI in all-cause mortality. Subsequently, subgroup analyses of gender and comorbidities were performed.Results1,386 AMI patients in the CCU were enrolled. The NLR was non-linearly and positively associated with in-hospital mortality [Q4: OR (95%CI) 2.61; (1.261–5.626), p = 0.012], 30-day mortality [Q4: OR (95%CI) 2.005; (1.048–3.925); p = 0.038], 90-day mortality [Q4: OR (95%CI) 2.191; (1.235–3.948); p = 0.008] with Q1 as the reference group. The NLR had the highest AUC for in-hospital mortality, 30-day mortality, and 90-day mortality among four inflammatory markers (NLR, SII, PLR, SIRI). Stratified analyses based on gender and comorbidities showed that the risk of death was significantly increased in male and female patients, with or without diabetes, without cerebral infarction, chronic obstructive pulmonary disease, liver disease, and renal disease in the Q4 group when compared to the Q1 group.ConclusionsNLR is nonlinearly and positively associated with all-cause mortality of AMI patients in the CCU. The predictive ability of NLR in in-hospital mortality, 30-day mortality, and 90-day mortality is superior to that of SII, PLR, and SIRI.
DNA-loaded targeted nanoparticles as a safe platform to produce exogenous proteins in tumor B cells
Authors: Grimaldi Maria Cristina , Bozzer Sara , Sjöström Dick J. , Andersson Linnea I. , Mollnes Tom Eirik , Nilsson Per H. , De Maso Luca , Riccardi Federico , Dal Bo Michele , Sblattero Daniele , Macor Paolo 
Published: January 2025

IntroductionThe functionalization of nanoparticles (NPs) with an antiCD19 targeting mechanism represents a promising approach for the selective delivery of drugs and nucleic acids into normal and tumor B cells. This strategy has the advantage of minimizing off-target effects by restricting gene delivery to the desired cell population. However, the nanoplatform must guarantee both the local production of the protein and the safety of the treatment to allow an effective therapy with reduced systemic toxicity.MethodsIn order to ensure a selective delivery of nucleic acids, we developed poly(lactic-co-glycolic acid) (PLGA)-poly(vinyl alcohol) (PVA) NPs loaded with an Enhanced Green Fluorescent Protein (EGFP)-coding plasmid and covalently coated with antiCD19 recombinant antibody as a targeting mechanism. To assess the functionality of the NPs, physicochemical characterization, safety tests, and transfection assay were employed to evaluate the NPs’ behavior in vitro and in vivo, in a human/zebrafish lymphoma xenograft model.ResultsThe results demonstrated that the PLGA-PVA nanoplatform was capable of efficiently encapsulating and releasing the payload. These nanostructures demonstrated a favorable safety profile, as evidenced by the absence of significant cell cytotoxicity, coagulation activation, complement system activation, and the slight activation of endothelial cells and leukocytes. The targeting mechanism facilitated the interaction of NPs with target cells, thereby enhancing their internalization and subsequent exogenous plasmid DNA (pDNA) translation and protein expression. In the human/zebrafish lymphoma xenograft model, no evidence of toxicity was observed, and targeted NPs demonstrated the capacity to enhance exogenous pDNA expression.ConclusionOur findings provide a rationale for the use of targeted NPs as a DNA delivery system for the local expression of therapeutic proteins.
Human inborn errors of immunity underlying Talaromyces marneffei infections: a multicenter, retrospective cohort study
Authors: Fan Huifeng , Yang Zhiyong , Wu Yuhui , Lu Xiulan , Li Tian , Lu Xuyang , Lu Gen , He Liming , Lu Guoping , Huang Li 
Published: January 2025

IntroductionTalaromyces marneffei (T. marneffei) infections in children can occur secondary to inborn errors of immunity (IEIs). We aimed to investigate the clinical and genetic features of T. marneffei infection in Chinese pediatric patients.Materials and methodsWe retrospectively reviewed 18 pediatric patients with IEIs who were diagnosed with T. marneffei infections at five public hospitals in China from January 2015 to January 2023.ResultsThe common clinical features among the patients were fever, cough, and hepatomegaly. The most common severe complications included septic shock, hemophagocytic lymphohistiocytosis (HLH), and acute respiratory distress syndrome (ARDS). Three cases presented with pan-hypogammaglobulinemia, while three other cases showed heightened levels of IgM. Elevated levels of IgE were detected in five cases, and six cases exhibited decreased T lymphocyte absolute counts. Four children were diagnosed with hyperimmunoglobulin M syndrome (HIGM) due to CD40LG mutations, three cases had severe combined immunodeficiency (SCID), and five were diagnosed with hyper-IgE syndrome (HIES). Gain-of-function (GOF) mutations in STAT1 led to STAT1 GOF in four cases. One patient was diagnosed with caspase-recruitment domain (CARD9) deficiency due to a compound mutation in the CARD9 gene, while another patient was confirmed with adenosine deaminase (ADA) deficiency.ConclusionT. marneffei infections in children with IEIs induced severe systemic complications. These children commonly exhibited abnormal immunoglobulin levels in peripheral blood, and underlying IEIs associated with T. marneffei infections have enhanced our understanding of the disease.
Cardiovascular toxicity of tisagenlecleucel in children and adolescents: analysis of spontaneous reports submitted to FAERS
Authors: Wang Ganggang , Su Lin , Liu Yanjun , Yang Xiaohan , Li Yi , Mei Qi , Gao Wen 
Published: January 2025

BackgroundThe advent of tisagenlecleucel has been a major advance in the pharmacological treatment of relapsed/refractory B-cell acute lymphoblastic leukemia in children and adolescents. However, further research is required to better define its safety profile.ObjectivesTo determine the cardiovascular toxicity of tisagenlecleucel in children and adolescents.MethodsThe US Food and Drug Administration’s Adverse Event Reporting System was searched to identify cardiovascular adverse events (CVAEs) related to tisagenlecleucel in pediatric patients up to the age of 18 years.ResultsThe median time to onset of tisagenlecleucel-associated CVAEs was shorter than that of tisagenlecleucel-associated non-CVAEs (3 days [interquartile range (IQR) 1, 6] vs. 7 days [IQR 2, 54]). The median time to onset was longer in patients with fatal CVAEs than in those with non-fatal CVAEs (4 days [IQR 1, 12.5] vs. 2 days [IQR 1, 4]). The most frequently reported CVAEs were mitral valve disease, hypotension, and capillary leak syndrome. Patients who developed shock had the highest mortality rate (66.67%). Concomitant use of medication for a neurological disorder was an independent risk factor for CVAEs, and concomitant use of medication for a respiratory disease was an independent risk factor for fatal CVAEs. Most CVAEs were associated with cytokine release syndrome, and older patients had a more favorable prognosis.ConclusionsChildren and adolescents who receive tisagenlecleucel should be closely monitored for CVAEs, particularly during the first week of treatment.
PDL1 inhibitors may be associated with a lower risk of allograft rejection than PD1 and CTLA4 inhibitors: analysis of the WHO pharmacovigilance database
Authors: Gérard Alexandre O. , Merino Diane , Benzaquen Jonathan , Destere Alexandre , Borchiellini Delphine , Gosset Clément , Rocher Fanny , Andreani Marine , Marquette Charles-Hugo , Montaudié Henri , Drici Milou-Daniel , Sicard Antoine 
Published: January 2025

BackgroundTransplant recipients face increased cancer mortality due to immunosuppressive treatments. Immune checkpoint inhibitors (ICI) have improved survival rates, but data on the use of these agents in transplant recipients is scarce. ICI may trigger allograft rejection, but the absolute risk of AR between the different ICI classes remains to be defined.MethodsVigiBase® (WHO’s pharmacovigilance database) was queried for reports of AR involving CTLA4, PD1, or PDL1 inhibitors. Disproportionality analysis compares the proportion of reports with a specific adverse drug reaction (ADR) and a given drug to the proportion of reports with the same ADR and other drugs. A lower 95% confidence interval for the Information Component (IC) &gt;0 suggests a signal. The comparative Reporting Odds Ratios (ROR) for AR, between PD1 and PDL1 inhibitors, was calculated.ResultsWe gathered 159 AR involving an ICI, especially nivolumab (73, 45.9%), mostly affecting kidneys (87, 54.7%). Median time to onset: 28 days. Fatal outcome: 36 reports (22.6%). ICI were significantly associated with AR: IC=1.7 [1.4;1.9]. Specifically, PD1 inhibitors yielded an IC of 2.0 [1.7;2.2] (152 reports observed compared to 38 expected). By contrast, the IC of PDL1 inhibitors was negative: -2.6 [-6.4;-1.0] (1 observed, 9 expected). The comparative ROR of PD1 compared to PDL1 inhibitors was 33.7 [4.7;240.9] (p=0.0005).ConclusionsWe confirm the association between ICI treatment and AR. Notably, PDL1 inhibitors showed surprisingly low AR reports compared to CTLA4 and PD1 inhibitors. Further prospective studies are warranted to confirm whether PDL1 inhibitors indeed reduce AR risk compared to other ICI.
Effectiveness rehabilitative therapy and Pridinol Mesylate in low back pain
Authors: Lauricella Lorenza , Calabrese Noemi , Scaturro Dalila , Migliorino Domenico , Vecchio Michele , Letizia Mauro Giulia 
Published: January 2025

BackgroundSpondylarthritis is a degenerative disease involving the intervertebral disc, vertebral bodies, and adjacent soft tissues. Treatment aims to slow disease progression and manage symptoms through an interdisciplinary approach. It can be conservative and rarely chirurgic.ObjectiveThis study aimed to evaluate the effectiveness of a rehabilitation combined with Pridinol Mesylate in the treatment of Spondylarthritis in elderly patients in terms of pain resolution, improving disability, and quality of life.Materials and methodsWe conducted a retrospective study on patients with Spondylarthritis. The patients recruited (n = 86) were divided into three groups: the Combined Group (CG:28), who received a rehabilitation combined with Pridinol Mesylate (16 women and 12 men, age 66.4 ± 3.99); the Rehabilitation Group (RG, n = 26), who received only rehabilitation (14 women and 12 men, age 66.2 ± 3.84); and the Drug Group (DG: 32), who received only the administration of the Pridinol(18 women and 14 men, age of 66.3 ± 3.9).ResultsThe results show, at T1 (20 days after treatment) in the CG, statistically significant improvements for the NRS and QBPDS. In the RG, statistically significant improvements were observed only for the QBPDS scale. In the DG group, only pain improvement. At T2 (90 days after treatment), the CG showed improvements in NRS, QBPDS, and (SF-36). The RG and DG showed improvements for NRS and for QBPDS. By Bonferroni method, obtained statistically significant values for CG versus RG and for CG versus DG. No statistical significance was found between RG versus DG.ConclusionTargeted rehabilitation treatment, combined with Pridinol Mesylate, reduced pain and improved disability in lumbar Spondylarthritis both in the short and medium term, with improved quality of life in elderly patients.
The amplitude of low frequency fluctuation and spontaneous brain activity alterations in age-related macular degeneration
Authors: Zhang Yan Yan , Hu Jin Yu , Ling Qian , Xu San Hua , Kang Min , Wei Hong , Zou Jie , Yi Quanyong , Tan Gang , Shao Yi 
Published: January 2025

BackgroundWet age-related macular degeneration (wAMD) is a vision-threatening eye disease worldwide. The amplitude of low-frequency fluctuation (ALFF) method was used to observe changes in spontaneous brain activity, which may help to investigate the underlying pathological mechanism of AMD.MethodsEighteen patients with wAMD and 18 age- and gender-matched healthy controls (HCs) were recruited. The ALFF method was used on each subject and mean ALFF values were compared between groups. The receiver operating characteristic (ROC) curve was used to compare the two groups.ResultsALFF values in the temporal lobe and limbic lobe/parahippocampal gyrus were significantly higher than controls, while values in the postcentral gyrus were significantly lower. The under the curve of the ROC (AUC) of the three regions shows high accuracy of the diagnosis.ConclusionThe abnormal spontaneous brain activity of patients with AMD suggests scope for the use of ALFF in the diagnosis or prognosis in AMD.
Risk factors for new vertebral fractures after percutaneous vertebroplasty or percutaneous kyphoplasty in the treatment of osteoporotic vertebral compression fractures
Authors: Yang Wencheng , Zou Kaiwei , Lin Xuping , Yang Yanfang , Chen Tianpei , Wu Xiuming , Wang Xiaomeng , Liu Qingjun , Huang Chunhui , Su Wanhan 
Published: January 2025

ObjectThis study aims to conduct a prospective analysis of patients with osteoporotic vertebral compression fractures (OVCF) who underwent percutaneous vertebroplasty (PVP) or percutaneous kyphoplasty (PKP), and further analyze the risk factors for new vertebral fracture following treatment.MethodsA prospective study was conducted from November 2020 to March 2022 at the First Hospital of Longyan City to select patients with OVCF who underwent treatment in the Department of Spinal Surgery. Data collection during the follow-up period focused on various factors that could potentially be associated with new vertebral fractures after PVP/PKP procedures. Patients were divided into two groups based on whether they experienced new vertebral fractures within two years after discharge: the new fracture group (n = 186) and the non-fracture group (n = 64), and statistical analysis was conducted accordingly.ResultsAll cases were followed up for 12 to 24 months, with an average of 14.7 months. Differential analysis revealed that age, diabetes, hemoglobin (HB), total protein (TP), serum albumin (ALB), b-C-terminal telopeptide of type I collage (β-CTX), 25-hydroxyvitamin D (25-OH-D3), number of fractured vertebrae, bone mineral density (BMD), regular exercise after discharge, anti-osteoporosis treatment after discharge, cross-sectional area (CSA), and fatty degeneration ratio (FDR) were associated with new vertebral fractures (all P &lt; 0.05). Multivariate analysis showed that age (OR = 1.519, P = 0.032), diabetes (OR = 3.273, P = 0.048), and FDR (OR = 1.571, P = 0.027) were positively associated with the occurrence of new vertebral fractures, while bone mineral density (OR = 0.108, P = 0.044), 25-OH-D3 (OR = 0.871, P = 0.032), CSA (OR = 0.564, P = 0.009), regular postoperative exercise (OR = 0.259, P = 0.025), and osteoporosis treatment (OR = 0.291, P = 0.045) were negatively associated with the occurrence of new vertebral fractures.ConclusionPatients with osteoporosis fractures who are older, have poor glycemic control, lower bone mineral density, lower levels of 25-OH-D3, weaker paraspinal muscles, and higher fat infiltration are at increased risk of new vertebral fractures after undergoing PKP/PVP. On the other hand, maintaining regular physical activity and adhering to osteoporosis treatment can help prevent new vertebral fractures.