bluuebunny/crossref_metadata_2025_split huggingface dataset:
Patients with bilateral patellar instability have multiple and symmetric risk factors in each knee
Authors: Parikh Shital N. , Rajdev Neil 
Published: September 2023

AbstractPurposeBilateral involvement is common in patients with patellar instability. The management of bilateral patellar instability is associated with increased complication rate. The higher complication rate in this cohort may be related to the presence of underlying anatomic risk factors. The purpose of the study was to evaluate the presence and side‐to‐side differences in risk factors between knees in bilateral patellar instability.MethodsIn a retrospective study (2008–2017), demographic information, characteristics of patellar dislocation and anatomic risk factors on MRI (trochlear dysplasia, patellar height, tibial tubercle lateralization, patellar tilt, sulcus angle, bump height) were evaluated in both knees of all patients (n = 32, 15 males and 17 females) with bilateral patellar instability. The risk factors were analyzed based on established cut off values and were compared between gender, laterality and more symptomatic (index) knee. Knee symmetry and absolute differences between risk factors for both knees were analyzed.ResultsThe mean age of 32 patients was 14.6 ± 2.3 years. Of the 4 major anatomic risk factors, the most common were trochlear dysplasia in 59/64 (92.1%) knees and patella alta in 51/64 (79.7%) knees. Tibial tubercle lateralization was the least common risk factor being present in 8/64 (12.5%) knees. Of 64 knees, 55 (85.9%) had 2 or more risk factors and 30 (46.8%) had 3 or all 4 risk factors present. There were no significant differences in risk factors based on gender, laterality or index knee. There was symmetry between paired knees for 31/32 (96.8%) patients for trochlear depth, 29/32 (90.6%) for patellar tilt, 27/32 (84.3%) for TT–TG distance and 25/32 (78.1%) for patellar height. There were no significant differences in absolute measurements between knees for any of the risk factors.ConclusionPatients with bilateral instability had multiple risk factors, with trochlear dysplasia being the most common and increased TT–TG distance being the least common. Majority of patients had 2 or more risk factors and about half had 3 or 4 risk factors in each knee. There was symmetry between paired knees for each risk factor without any significant differences between the index knee compared to the contralateral knee.Level of evidenceLevel III.
Schnittstellenmanagement in der manuellen Medizin
Authors: Winkelmann Claudia , Rogalski Christina 
Published: September 2023

ZusammenfassungIm Rahmen der manuellen Medizin bestehen sowohl innerhalb einer Einrichtung wie Arztpraxis als auch nach außen wie Klinik oder Praxis für Physiotherapie Schnittstellen. Die Patientenversorgung erfolgt häufig als Zusammenarbeit von Facharzt und Physiotherapeut. Dabei kann die Arbeitsteilung in der manuellen Diagnostik und Verordnung durch den Facharzt sowie in der manuellen Therapie durch den Physiotherapeuten bestehen. Immer dann, wenn unterschiedliche Einrichtungen und/oder Sektoren und/oder Personen im Versorgungsprozess beteiligt sind, kommt es an den Grenzen zu Brüchen. Diese Grenzen respektive Schnittstellen gilt es mit Blick auf die optimale, qualitätsgesicherte Patientenversorgung so zu gestalten, dass die Prozesse der Übergänge möglichst reibungslos verlaufen. Aus betriebswirtschaftlicher Perspektive bezieht sich dies auf die drei Faktoren Qualität, Zeit und Kosten. Schnittstellenmanagement zählt zum Qualitätsmanagement. Ziel des Beitrags ist es, praxisrelevante Punkte zum Management vorhandener Schnittstellen zur optimalen Versorgung von Patienten im Rahmen der manuellen Medizin und Therapie herauszuarbeiten. Dabei werden die Fragen, weshalb ein Schnittstellenmanagement erforderlich ist und welche Aspekte für die Praxis relevant sind, sekundärdatenanalytisch untersucht. Wesentliches Ergebnis ist, dass es Best Practice, insbesondere im Kontext des Projektmanagements, gibt. Allerdings sind für jede Einrichtung im Rahmen der Qualitätssicherung die Schnittstellen und deren optimales Management festzulegen. Voraussetzungen sind das Organigramm mit der Definition von Verantwortungsbereichen sowie nachfolgend die Prozessbeschreibungen inklusive Erstellen von Dokumenten. Das Vorgehen nach dem Prinzip „Das haben wir schon immer so gemacht“ weicht vom vorstehenden Vorgehen insofern ab, als dass hier die Struktur den bestehenden Abläufen angepasst wird. Im Rahmen des Qualitätsmanagements, insbesondere im Zuge von Zertifizierungen, kann sich das Hinterfragen und Neuausrichten von Strukturen und Prozessen anbieten.
Flickering stimuli presentation in imprinting
Authors: Lemaire Bastien S. , Zanon Mirko , Placì Sarah , Werk Benjamin , Vallortigara Giorgio 
Published: September 2023

AbstractImprinting, the process of forming lasting social bonds with early encountered stimuli, has been the subject of extensive research. However, there is still a need to systematically study the optimal methods for displaying imprinting stimuli in laboratory settings. This study aimed to investigate the effectiveness of different virtual presentation methods for imprinting stimuli and their impact on the memory of chicks. In the first experiment, we examined the attractiveness of various flickering frequencies, comparing them to static and translatory motion stimuli. The results revealed that flickering frequencies between 0.5 and 5 Hz were particularly appealing to newly hatched chicks, while higher frequencies (10–40 Hz) were less effective. We observed no significant differences in attractiveness between low flickering frequencies, moving stimuli, and static stimuli. In the second experiment, the focus shifted to the development of imprinting preference and memory. We found no significant difference in terms of preference for the imprinting stimulus between chicks imprinted with translatory motion or static stimuli. However, imprinting with flickering stimuli produced varied preferences. Chicks imprinted with a 2 Hz flickering stimulus exhibited a preference for the imprinting stimulus, albeit weaker than those imprinted with moving stimuli, while chicks imprinted with a 1 Hz flickering stimulus did not show a preference. These findings suggest that imprinting with flickering frequencies is not as effective as imprinting with moving stimuli and, to a lesser extent, static stimuli. Future studies should aim to determine the most optimal low frequencies within the 0.5–5 Hz range and explore different motion types. Overall, this research enhances our understanding of imprinting and provides valuable insights into virtual stimulation methods, thus informing the design of experiments in virtual environments.
Baseline interleukin-6 is a prognostic factor for patients with metastatic breast cancer treated with eribulin
Authors: Bun Ayako , Nagahashi Masayuki , Kuroiwa Mamiko , Komatsu Miki , Miyoshi Yasuo 
Published: September 2023

Abstract
Purpose
Eribulin is a unique anti-cancer drug which can improve overall survival (OS) of patients with metastatic breast cancer (MBC), probably by modulating the tumor immune microenvironment. The aim of this study was to investigate the clinical significance of serum levels of immune-related and inflammatory cytokines in patients treated with eribulin. Furthermore, we investigated the association between cytokines and immune cells, such as myeloid-derived suppressor cells (MDSCs) and cytotoxic and regulatory T cells, to explore how these cytokines might affect the immune microenvironment.

Methods
Sixty-eight patients with MBC treated with eribulin were recruited for this retrospective study. The relationship of cytokines, including interleukin (IL)-6, to progression-free survival and OS was examined. CD4+ and CD8+ lymphocyte, MDSCs and regulatory T cell levels were determined in the blood by flow cytometry analysis.

Results
In our cohort, patients with high IL-6 at baseline had shorter progression-free survival and OS compared with those with low IL-6 (p = 0.0017 and p = 0.0012, respectively). Univariable and multivariable analyses revealed that baseline IL-6 was an independent prognostic factor for OS (p = 0.0058). Importantly, CD8+ lymphocytes were significantly lower and MDSCs were significantly higher in patients with high IL-6, compared to those with low IL-6.

Conclusion
Baseline IL-6 is an important prognostic factor in patients with MBC treated with eribulin. Our results show that high IL-6 is associated with higher levels of MDSCs which suppress anti-tumor immunity, such as CD8+ cells. It appears that eribulin is not particularly effective in patients with high IL-6 due to a poor tumor immune microenvironment.
Improving performance of natural rubber composites by the application of functional biofiller: horsetail modified with silane coupling agents
Authors: Miedzianowska Justyna , Masłowski Marcin , Strzelec Krzysztof 
Published: September 2023

AbstractThe growing ecological awareness of society and increasingly stringent legal requirements regarding environmental protection and the strategy of implementing the principles of sustainable development force the search for and continuous development of environmentally friendly solutions in the field of polymer materials technology. One of the directions is the use of raw materials from renewable sources. For this reason, the research object of the presented work was natural rubber composites containing a filler of plant origin in the form of ground horsetail (HT) biomass. Despite its health-promoting properties, it is commonly considered a weed. What’s more, the ubiquitous occurrence and surplus make it a valuable source of waste biomass for management. Taking into account the limitations resulting from the hydrophilic nature of the lignocellulosic filler, and the related poor adhesion to the non-polar elastomer matrix, the horsetail filler was modified with organoalkoxysilanes. The study used silane compounds containing groups that can affect the properties of vulcanizates in various ways, such as vinyltriethoxysilane (VTES), 3,3′-Tetrathiobis(propyl-triethoxysilane) (TESPTS), 3-(aminopropyl)triethoxysilane (APTES), 3-(chloropropyl)triethoxysilane (CPTES) and octyltriethoxysilane (OTES). The biomass in the form of field horsetail was previously modified with selected silanes. Then, the bioadditive prepared in this way was applied to rubber mixtures. The process of modifying the natural filler contributed to structural changes in the lignocellulosic material, which may indicate the effective attachment of silane compounds to the horsetail surface. The results of the contact angle analysis show that the treatment strongly influenced the surface characteristics of the fillers, making them more hydrophobic. The results show that the type of silane coupling agent affects not only the processing associated with the vulcanization process but also the mechanical properties of the NR vulcanizates. This phenomenon is probably the result of increased rubber-bioadditive interaction and improved filler dispersion. Moreover, all composites with modified HT, show a greater flame permanence time than the one using unmodified filler.
Graphical abstract
Evaluation of Pile Driving Effects on Slope Stability in Clay
Authors: Attari Yeganeh , Jostad Hans Petter , Grimstad Gustav , Eiksund Gudmund Reidar 
Published: September 2023

AbstractPile driving can trigger slope failure, specifically in sensitive soil deposits or marginally stable slopes. Cases of slope failure during pile installation have been reported in countries such as Norway, Sweden and Canada where soft sensitive clay deposits are prevalent. Although a few approaches have been suggested to assess the factor of safety of a slope during pile driving, there is currently no standard method recognized by the geotechnical society to account for the reduction of safety factor of a slope as an effect of pile installation. Some approaches suggest using the excess pore pressure generated during pile driving to account for the reduction in soil strength (by reducing effective stresses), neglecting the change in total stresses. This paper discusses the accuracy of stability analysis methods currently in use for this problem by looking at stress changes in the soil during pile driving as well as using numerical analysis results. Importantly, the paper identifies the shortcomings regarding these approaches, thus allowing geo-engineers to understand the limitations of their analysis and the uncertainties in the models.
Nature reserves (zapovedniks) in the Volga catchment: protection, research and management
Authors: Schletterer M. , Kuzovlev V. V. , Zheltukhin A. S. , Litvinov K. V. , Osipov V. V. , Ruchin A. B. 
Published: September 2023

AbstractIn the Russian Federation, the protection category “Zapovednik” represents a strictly protected area, where the access is restricted and the aim is to keep the area “forever wild”. We provide an overview about the organization of these nature reserves and highlight their contribution to fundamental research. Aquatic ecosystems in zapovedniks provide pristine sites of great importance for long-term monitoring and research, which fosters process understanding regarding interannual variability and development of aquatic coenosis. We present a long term monitoring and research project about abiotic and biotic parameters in the Central Forest State Nature Biosphere Reserve. Examples from the Privolzhskaya Lesostep Nature Reserve include monitoring of invasive fish as well as analyses about the influence of beavers on fish. The Astrakhan Biosphere Nature Reserve in the Volga delta is a hotspot for fish biodiversity; thus, there the state nature reserve security service plays an important role to avoid poaching. Our case studies from the Volga Basin exemplify the structure and function of nature reserves and highlight their contribution to research, protection and management of aquatic ecosystems. Long-term datasets from the zapovedniks can (i) improve process understanding, (ii) ensure protection of aquatic systems and sustainable water management and (iii) support knowledge-based decisions.
Annotation Protocol for Textbook Enrichment with Prerequisite Knowledge Graph
Authors: Alzetta Chiara , Torre Ilaria , Koceva Frosina 
Published: September 2023

AbstractExtracting and formally representing the knowledge embedded in textbooks, such as the concepts explained and the relations between them, can support the provision of advanced knowledge-based services for learning environments and digital libraries. In this paper, we consider a specific type of relation in textbooks referred to as prerequisite relations (PR). PRs represent precedence relations between concepts aimed to provide the reader with the knowledge needed to understand a further concept(s). Their annotation in educational texts produces datasets that can be represented as a graph of concepts connected by PRs. However, building good-quality and reliable datasets of PRs from a textbook is still an open issue, not just for automated annotation methods but even for manual annotation. In turn, the lack of good-quality datasets and well-defined criteria to identify PRs affect the development and validation of automated methods for prerequisite identification. As a contribution to this issue, in this paper, we propose PREAP, a protocol for the annotation of prerequisite relations in textbooks aimed at obtaining reliable annotated data that can be shared, compared, and reused in the research community. PREAP defines a novel textbook-driven annotation method aimed to capture the structure of prerequisites underlying the text. The protocol has been evaluated against baseline methods for manual and automatic annotation. The findings show that PREAP enables the creation of prerequisite knowledge graphs that have higher inter-annotator agreement, accuracy, and alignment with text than the baseline methods. This suggests that the protocol is able to accurately capture the PRs expressed in the text. Furthermore, the findings show that the time required to complete the annotation using PREAP are significantly shorter than with the other manual baseline methods. The paper includes also guidelines for using PREAP in three annotation scenarios, experimentally tested. We also provide example datasets and a user interface that we developed to support prerequisite annotation.
Parental Support and Adolescents’ Coping with Academic Stressors: A Longitudinal Study of Parents’ Influence Beyond Academic Pressure and Achievement
Authors: Zimmer-Gembeck Melanie J. , Skinner Ellen A. , Scott Riley A. , Ryan Katherine M. , Hawes Tanya , Gardner Alex A. , Duffy Amanda L. 
Published: September 2023

AbstractAdolescents face many academic pressures that require good coping skills, but coping skills can also depend on social resources, such as parental support and fewer negative interactions. The aim of this study was to determine if parental support and parental negative interactions concurrently and longitudinally relate to adolescents’ ways of academic coping, above and beyond the impact of three types of academic stress, students’ achievement at school (i.e., grades in school), and age. Survey data were collected from 839 Australian students in grades 5 to 10 (Mage = 12.2, SD = 1.72; 50% girls). Students completed measures of support and negative interactions with parents; academic stress from workload, external pressure (teachers/parents) to achieve, and intrapsychic pressure for high achievement; and ways of academic coping that were grouped into two positive and two negative types. Hypothesized associations were tested concurrently and from one year to the next using path modeling. Beyond the numerous significant influences of academic stress and achievement on coping, and control for age and COVID-19 timing, adolescents with more parental support reported more use of engagement coping (e.g., strategizing) and comfort-seeking, whereas those who reported more negative interactions with parents reported more use of disengagement coping (e.g., concealment) and escape. In the longitudinal model, parental support predicted an increase in engagement and comfort-seeking and a decrease in disengagement coping, whereas negative interaction with parents predicted an increase in disengagement coping. Overall, the findings support the view that coping with academic stressors will continue to depend on parent-adolescent relationships even into the teen years.
In vivo CRISPR/LbCas12a-mediated knock-in and knock-out in Atlantic salmon (Salmo salar L.)
Authors: Raudstein Mari , Kjærner-Semb Erik , Barvik Morten , Broll Silje , Straume Anne Hege , Edvardsen Rolf Brudvik 
Published: September 2023

AbstractGenome editing using the CRISPR/Cas system offers the potential to enhance current breeding programs and introduce desirable genetic traits, including disease resistance, in salmon aquaculture. Several nucleases are available using this system, displaying differences regarding structure, cleavage, and PAM requirement. Cas9 is well established in Atlantic salmon, but Cas12a has yet to be tested in vivo in this species. In the present work, we microinjected salmon embryos with LbCas12a ribonucleoprotein complexes targeting the pigmentation gene solute carrier family 45 member 2 (slc45a2). Using CRISPR/LbCas12a, we were able to knock-out slc45a2 and knock-in a FLAG sequence element by providing single-stranded DNA templates. High-throughput sequencing revealed perfect HDR rates up to 34.3% and 54.9% in individual larvae using either target or non-target strand template design, respectively. In this work, we demonstrate the in vivo application of CRISPR/LbCas12a in Atlantic salmon, expanding the toolbox for editing the genome of this important aquaculture species.
Growth patterns of Pseudomonas aeruginosa in milk fortified with chitosan and selenium nanoparticles during refrigerated storage
Authors: Atia Rehab M. , Mohamed Hamdi A. , AboELRoos Nahla A. , Awad Dina A. B. 
Published: September 2023

AbstractPseudomonas spp are considered a common milk-associated psychotropic bacteria, leading to milk deterioration during storage; therefore, our study aimed to study the distribution of Pseudomonas aeruginosa in raw milk and its associated products then studying the growth behavior of P. aeruginosa in milk after employing chitosan nanoparticles (CsNPs 50, 25, and 15 mg/100ml) and selenium nanoparticles (SeNPs 0.5, 0.3 and 0.1 mg/100ml) as a trial to control the bacterial growth in milk during five days of cooling storage. Our study relies on the ion gelation method and green synthesis for the conversion of chitosan and selenium to nanosized particles respectively, we subsequently confirmed their shape using SEM and TEM. We employing Pseudomonas selective agar medium for monitoring the bacterial growth along the cooling storage. Our findings reported that high prevalence of Pseudomonas spp count in raw milk and kareish cheese and high incidence percent of P. aeruginosa in ice cream and yogurt respectively. Both synthesized nanoparticles exhibited antibacterial activity in a dose-dependent manner. Moreover, CsNPs50 could inhibit the P. aeruginosa survival growth to a mean average of 2.62 ± 1.18 log10cfu/ml in the fifth day of milk cooling storage; also, it was noted that the hexagonal particles SeNPs0.5 could inhibit 2.49 ± 11 log10cfu/ml in comparison to the control P. aeruginosa milk group exhibited growth survival rate 7.24 ± 2.57 log10cfu/ml under the same conditions. In conclusion, we suggest employing chitosan and selenium nanoparticles to improve milk safety and recommend future studies for the fate of nanoparticles in milk.
Mesenchymal Stem Cells Isolated from Equine Hair Follicles Using a Method of Air-Liquid Interface
Authors: Li Hanluo , Xiong Shiwen , Masieri Federica Francesca , Monika Seltenhammer , Lethaus Bernd , Savkovic Vuk 
Published: September 2023

AbstractEquine mesenchymal stem cells (MSC) of various origins have been identified in horses, including MSCs from the bone marrow and adipose tissue. However, these stem cell sources are highly invasive in sampling, which thereby limits their clinical application in equine veterinary medicine. This study presents a novel method using an air-liquid interface to isolate stem cells from the hair follicle outer root sheath of the equine forehead skin. These stem cells cultured herewith showed high proliferation and asumed MSC phenotype by expressing MSC positive biomarkers (CD29, CD44 CD90) while not expressing negative markers (CD14, CD34 and CD45). They were capable of differentiating towards chondrogenic, osteogenic and adipogenic lineages, which was comparable with MSCs from adipose tissue. Due to their proliferative phenotype in vitro, MSC-like profile and differentiation capacities, we named them equine mesenchymal stem cells from the hair follicle outer root sheath (eMSCORS). eMSCORS present a promising alternative stem cell source for the equine veterinary medicine.
Graphical abstract