bluuebunny/crossref_metadata_2025_split huggingface dataset:
Metalloproteome of human-infective RNA viruses: a study towards understanding the role of metal ions in virology
Authors: Dixit Himisha , Kulharia Mahesh , Verma Shailender Kumar 
Published: September 2023

Abstract
Metalloproteins and metal-based inhibitors have been shown to effectively combat infectious diseases, particularly those caused by RNA viruses. In this study, a diverse set of bioinformatics methods was employed to identify metal-binding proteins of human RNA viruses. Seventy-three viral proteins with a high probability of being metal-binding proteins were identified. These proteins included 40 zinc-, 47 magnesium- and 14 manganese-binding proteins belonging to 29 viral species and eight significant viral families, including Coronaviridae, Flaviviridae and Retroviridae. Further functional characterization has revealed that these proteins play a critical role in several viral processes, including viral replication, fusion and host viral entry. They fall under the essential categories of viral proteins, including polymerase and protease enzymes. Magnesium ion is abundantly predicted to interact with these viral enzymes, followed by zinc. In addition, this study also examined the evolutionary aspects of predicted viral metalloproteins, offering essential insights into the metal utilization patterns among different viral species. The analysis indicates that the metal utilization patterns are conserved within the functional classes of the proteins. In conclusion, the findings of this study provide significant knowledge on viral metalloproteins that can serve as a valuable foundation for future research in this area.
Function analysis of CYP321A9 from Spodoptera frugiperda (Lepidoptera: Noctuidae) associated with emamectin benzoate, and a novel insecticide, cyproflanilide detoxification
Authors: Shi Yang , He Li , Ding Wenbing , Huang Hong , He Hualiang , Xue Jin , Gao Qiao , Zhang Zhixiang , Li Youzhi , Qiu Lin 
Published: August 2023

Abstract
The fall armyworm, Spodoptera frugiperda, is an invasive agricultural pest that is a serious threat to agricultural production and global food security. Chemical control is the most effective method for preventing outbreaks of S. frugiperda. However, insecticide resistance often develops as a result of prolonged pesticide use, and the molecular mechanisms involved in insecticide resistance remain unclear. Insect cytochrome P450 monooxygenases play an important role in the detoxification of insecticides and insecticide resistance in Lepidoptera. In our study, the LC50 of a novel insecticide (cyproflanilide) and a conventional insecticide (emamectin benzoate) for S. frugiperda second-instar larvae were 7.04 and 1.61 mg/L, respectively. Furthermore, CYP321A9 expression was upregulated in larvae exposed to these insecticides. Additionally, knockdown of CYP321A9 by feeding larvae with dsRNA for 72 h significantly increased the mortality of S. frugiperda exposed to emamectin benzoate and cyproflanilide by 23.33% and 7.78%, respectively. Our results indicate that CYP321A9 may play an important role in the detoxification of emamectin benzoate and cyproflanilide in S. frugiperda. Our findings provide a basis to better understand the mechanisms of insecticide resistance and contribute to the control of S. frugiperda.
Twentieth-century private collecting
Authors: Muskett Georgina 
Published: September 2023

Abstract
Dr Philip Nelson, who died in 1953, was an antiquary and collector who lived in Liverpool. One of the highlights of the collections of classical sculpture in the World Museum, part of National Museums Liverpool, is a group of statuettes and busts acquired by Nelson from the 12th Lord Kinnaird and formerly housed in Rossie Priory, near Perth in Scotland. Although Nelson bought only two sculptures from the first auction of sculptures from the Rossie Priory collection, held at Sotheby’s in London in December 1948, it is apparent that he acquired several others, either directly from Lord Kinnaird or through dealers. Details of this process can be found in the archives of the World Museum and the Walker Art Gallery, also part of National Museums Liverpool, including hitherto unpublished letters, notebooks and invoices, reproduced here in an online appendix. The correspondence provides an insight into the formation of a private collection of ancient sculpture in the first half of the twentieth century, a period often overlooked in collecting scholarship.
Massive mixed epithelial-stromal tumour of seminal vesicle requiring challenging abdominoperineal resection: a case report and review of literature
Authors: Yu Zirong , Choy Kay Tai , Ong Ferdinand , Williams Evan , Naidu Sanjeev , Smithers Bernard M , Gurung Anjan , Lutton Nicholas 
Published: September 2023

Abstract
Mixed epithelial-stromal tumours (MESTs) are a rare biphasic tumour that frequently arise in women from the renal and urogenital tract. They are also seen in men but are exceptionally uncommon with only few cases reported to originate from the seminal vesicles. Malignant transformation of its epithelial or stromal components is possible; however, by in large, these tumours are benign in nature. We report the case of a 48-year-old man with no remarkable medical or surgical history who presented with a huge expanding pelvic and intra-abdominal mass that required extensive surgical management including a pelvic exenteration. Histopathological analysis concluded the diagnosis of benign MEST originating from the seminal vesicles with no malignant features. No further systemic therapy was recommended for our patient. Given the technical intricacy in the operative resection of this tumour, we aim to present our findings and surgical management of this complex MEST.
The WD40 domain-containing protein Ehd5 positively regulates flowering in rice (Oryza sativa)
Authors: Zhang Xuening , Feng Qi , Miao Jiashun , Zhu Jingjie , Zhou Congcong , Fan Danlin , Lu Yiqi , Tian Qilin , Wang Yongchun , Zhan Qilin , Wang Zi-Qun , Wang Ahong , Zhang Lei , Shangguan Yingying , Li Wenjun , Chen Jiaying , Weng Qijun , Huang Tao , Tang Shican , Si Lizhen , Huang Xuehui , Wang Zi-Xuan , Han Bin 
Published: August 2023

Abstract
Heading date (flowering time), which greatly influences regional and seasonal adaptability in rice (Oryza sativa), is regulated by many genes in different photoperiod pathways. Here, we characterized a heading date gene, Early heading date 5 (Ehd5), using a modified bulked segregant analysis method. The ehd5 mutant showed late flowering under both short-day and long-day conditions, as well as reduced yield, compared to the wild type. Ehd5, which encodes a WD40 domain-containing protein, is induced by light and follows a circadian rhythm expression pattern. Transcriptome analysis revealed that Ehd5 acts upstream of the flowering genes Early heading date 1 (Ehd1), RICE FLOWERING LOCUS T 1 (RFT1), and Heading date 3a (Hd3a). Functional analysis showed that Ehd5 directly interacts with Rice outermost cell-specific gene 4 (Roc4) and Grain number, plant height, and heading date 8 (Ghd8), which might affect the formation of Ghd7–Ghd8 complexes, resulting in increased expression of Ehd1, Hd3a, and RFT1. In a nutshell, these results demonstrate that Ehd5 functions as a positive regulator of rice flowering and provide insight into the molecular mechanisms underlying heading date.
MYB24 orchestrates terpene and flavonol metabolism as light responses to anthocyanin depletion in variegated grape berries
Authors: Zhang Chen , Dai Zhanwu , Ferrier Thilia , Orduña Luis , Santiago Antonio , Peris Arnau , Wong Darren C J , Kappel Christian , Savoi Stefania , Loyola Rodrigo , Amato Alessandra , Kozak Bartosz , Li Miaomiao , Liang Akun , Carrasco David , Meyer-Regueiro Carlos , Espinoza Carmen , Hilbert Ghislaine , Figueroa-Balderas Rosa , Cantu Dario , Arroyo-Garcia Rosa , Arce-Johnson Patricio , Claudel Patricia , Errandonea Daniel , Rodríguez-Concepción Manuel , Duchêne Eric , Huang Shao-shan Carol , Castellarin Simone Diego , Tornielli Giovanni Battista , Barrieu Francois , Matus José Tomás 
Published: August 2023

Abstract
Variegation is a rare type of mosaicism not fully studied in plants, especially fruits. We examined red and white sections of grape (Vitis vinifera cv. ‘Béquignol’) variegated berries and found that accumulation of products from branches of the phenylpropanoid and isoprenoid pathways showed an opposite tendency. Light-responsive flavonol and monoterpene levels increased in anthocyanin-depleted areas in correlation with increasing MYB24 expression. Cistrome analysis suggested that MYB24 binds to the promoters of 22 terpene synthase (TPS) genes, as well as 32 photosynthesis/light-related genes, including carotenoid pathway members, the flavonol regulator HY5 HOMOLOGUE (HYH), and other radiation response genes. Indeed, TPS35, TPS09, the carotenoid isomerase gene CRTISO2, and HYH were activated in the presence of MYB24 and MYC2. We suggest that MYB24 modulates ultraviolet and high-intensity visible light stress responses that include terpene and flavonol synthesis and potentially affects carotenoids. The MYB24 regulatory network is developmentally triggered after the onset of berry ripening, while the absence of anthocyanin sunscreens accelerates its activation, likely in a dose-dependent manner due to increased radiation exposure. Anthocyanins and flavonols in variegated berry skins act as effective sunscreens but for different wavelength ranges. The expression patterns of stress marker genes in red and white sections of ‘Béquignol’ berries strongly suggest that MYB24 promotes light stress amelioration but only partly succeeds during late ripening.
Color Flow Doppler in Spinal Ultrasound: A Novel Technique for Assessment of Catheter Position in Labor Epidurals
Authors: van den Bosch O.F.C. , Gleicher Y. , Arzola C. , Siddiqui N. , Downey K. , Carvalho J.C.A. 
Published: September 2023

(Reg Anesth Pain Med 2022;47:775–779)
Epidural analgesia is one of the most common pain relief treatments during labor and delivery and is usually very effective. In a small percentage of epidurals, misplacement of the epidural catheter can cause inadequate anesthetic effects. To reduce instances of insufficient pain relief, it would be beneficial to be able to assess the position and flow of the catheter before administering medication. This study was designed to visualize flow in the epidural space using a structured color flow Doppler assessment, and to describe the location of the flow relative to the catheter insertion site. The primary outcome was the visualization of flow in the epidural space by color flow Doppler ultrasound. Secondary outcomes were visualization of catheter flow at the interspace of insertion, and at the interspaces above and below insertion, as well as visibility from unilateral or bilateral view, and visibility of flow on injection of saline only or an injection of a saline and air mixture.
Preparing for Obstetric Anesthesia: An Educational Randomised Controlled Trial Comparing E-learning to Written Course Material
Authors: Andersson M.L. , Duch P. , Bessmann E.L. , Lundstrøm L.H. , Ekelund K. 
Published: September 2023

(Acta Anaesthesiol Scand. 2023;67:36–43)
E-learning is becoming more common in postgraduate continual medical education. While there many studies that have shown the benefits of e-learning and its effectiveness has similarity to traditional teaching methods, the studies vary in quality. This study aimed to determine whether e-learning better prepares obstetric anesthesia course participants compared with written course material by assessing precourse knowledge, anxiety level, group performance, and postcourse knowledge.
Association of Texas’ 2021 Ban on Abortion in Early Pregnancy With the Number of Facility-based Abortions in Texas and Surrounding States
Authors: White K. , Sierra G. , Lerma K. , Beasley A. , Hofler L.G. , Tocce K. , Goyal V. , Ogburn T. , Potter J.E. , Dickman S.L. 
Published: September 2023

(JAMA. 2022;328:2048–2055)
In 2021, the state of Texas implemented a strict abortion law (SB 8) that banned abortion after the detection of fetal cardiac activity. Previously, abortions could be performed in the state of Texas up to 22 weeks of gestation. In June 2022, the US Supreme Court overturned its decision on Roe v Wade, making it possible to enforce the new legislation banning nearly all abortions. Primary outcomes for this study included the change in the number of facility-based, out-of-state, and total documented abortions for Texas residents after the implementation of SB 8. The secondary outcome was the change in the percentage of out-of-state abortions for Texas residents that were provided at or after 12 weeks of gestation.
Delivering Evidence to Prevent Recurrent Venous Thromboembolism in Pregnancy
Authors: van Lennep J.E.R. , Nerenberg K.A. 
Published: September 2023

(Lancet. 2022;400:1743–1745)
Pregnant and postpartum women have an increased risk for venous thromboembolism (VTE) (0 to 1%) with a history of VTE increasing the likelihood (2% to 10%). Hypercoagulability, endothelial damage, and venous stasis are contributing factors. Low–molecular-weight heparin (LMWH) thromboprophylaxis during pregnancy to 6 weeks postpartum is the recommended treatment for women with history of VTE, yet evidence-based dosage recommendations are lacking, causing dosage variation between countries and practices.
Put Your Weight Behind It—Effect of Body Mass Index on the Active Second Stage of Labor: A Retrospective Cohort Study
Authors: Østborg T.B. , Sande R.K. , Kessler J. , Tappert C. , von Brandis P. , Eggebø T.M. 
Published: September 2023

(BJOG. 2022;129:2166–2174)
Recent studies have indicated rising rates of obesity throughout the world, with larger percentages of women in developed countries being classified as overweight or obese. Obesity in pregnancy has been associated with complications such as pre-eclampsia, gestational diabetes, preterm delivery, and others. Obesity is also associated with delivery complications and increases risk of surgical intervention. Previous research has shown an association between obesity and slow labor progress, and awareness of the risks associated with obesity may bias both the mother and the birth attendant, affecting both labor experience and potentially outcome. The purpose of this study was to examine differences in the active second stage of labor in groups based on body mass index (BMI), with the primary outcome the median duration of the second stage.
Expectant Management or Early Ibuprofen for Patent Ductus Arteriosus
Authors: Hundscheid T. , Onland W. , Kooi E.M.W. , Vijlbrief D.C. , de Vries W.B. , de Boode W.P. ,   BeNeDuctus Trial Investigators
Published: September 2023

(N Engl J Med. 2023;388:980–990)
Patent ductus arteriosus (PDA), where the vessel connecting the pulmonary artery and aorta does not properly close, is a common adverse outcome for preterm birth. Cyclooxygenase inhibitors are used to help treat PDA but the evidence and clinical outcome benefits are lacking. This study aimed to answer the question whether expectant management or ibuprofen treatment in extremely preterm infants with PDA are a superior treatment.
Interventions for the Prevention or Treatment of Epidural-related Maternal Fever: A Systematic Review and Meta-analysis
Authors: Cartledge A. , Hind D. , Bradburn M. , Martyn-St James M. , Davenport S. , Tung W.S. , Yung H. , Wong J. , Wilson M. 
Published: September 2023

(British Journal of Anaesthesia. 2022;129:567e580)
Epidural-related maternal fever (ERMF) occurs in 15% to 25% of laboring women who receive analgesia. ERMF is not seen in non-pregnant or pregnant women who are not in labor. The cause behind ERMF is not yet understood. There have been studies on a number of preventative interventions for ERMF. This systematic review aimed to compare the effectiveness of proposed interventions, primarily in terms of the incidence of ERMF, and secondarily, by comparing the incidence of neonatal sepsis evaluation and neonatal admission to level 2 care.
Individualized Music Interventions for Dementia-Related Behavior Management
Authors: Simpson Jeannie 
Published: September 2023

ABSTRACT:
Dementia presents many challenges for the person diagnosed, as well as for caregivers. The use of psychotropic medications for dementia-related behaviors has been linked to adverse reactions and increased mortality. Alternative approaches such as the use of personalized music playlists and musically-related spiritual care have had promising results including improved quality of life for persons with dementia and reduced stress for caregivers. Implementing an individualized music program is relatively simple and avoids deleterious side effects that may accompany medication use.
Effects of individualised positive end-expiratory pressure titration on respiratory and haemodynamic parameters during the Trendelenburg position with pneumoperitoneum
Authors: Boesing Christoph , Schaefer Laura , Schoettler Jochen Johannes , Quentin Alena , Beck Grietje , Thiel Manfred , Honeck Patrick , Kowalewski Karl-Friedrich , Pelosi Paolo , Rocco Patricia R.M. , Luecke Thomas , Krebs Joerg 
Published: August 2023

BACKGROUND
The Trendelenburg position with pneumoperitoneum during surgery promotes dorsobasal atelectasis formation, which impairs respiratory mechanics and increases lung stress and strain. Positive end-expiratory pressure (PEEP) can reduce pulmonary inhomogeneities and preserve end-expiratory lung volume (EELV), resulting in decreased inspiratory strain and improved gas-exchange. The optimal intraoperative PEEP strategy is unclear.

OBJECTIVE(S)
To compare the effects of individualised PEEP titration strategies on set PEEP levels and resulting transpulmonary pressures, respiratory mechanics, gas-exchange and haemodynamics during Trendelenburg position with pneumoperitoneum.

DESIGN
Prospective, randomised, crossover single-centre physiologic trial.

SETTING
University hospital.

PATIENTS
Thirty-six patients receiving robot-assisted laparoscopic radical prostatectomy.

INTERVENTION(S)
Randomised sequence of three different PEEP strategies: standard PEEP level of 5 cmH2O (PEEP5), PEEP titration targeting a minimal driving pressure (PEEP
Δ
P
) and oesophageal pressure-guided PEEP titration (PEEPPoeso) targeting an end-expiratory transpulmonary pressure (P
TP) of 0 cmH2O.

MAIN OUTCOME MEASURES
The primary endpoint was the PEEP level when set according to PEEP
Δ
P
and PEEPPoeso compared with PEEP of 5 cmH2O. Secondary endpoints were respiratory mechanics, lung volumes, gas-exchange and haemodynamic parameters.

RESULTS
PEEP levels differed between PEEP
Δ
P
, PEEPPoeso and PEEP5 (18.0 [16.0 to 18.0] vs. 20.0 [18.0 to 24.0]vs. 5.0 [5.0 to 5.0] cmH2O; P < 0.001 each). End-expiratory P
TP and lung volume were lower in PEEP
Δ
P
compared with PEEPPoeso (P = 0.014 and P < 0.001, respectively), but driving pressure, lung stress, as well as respiratory system and dynamic elastic power were minimised using PEEP
Δ
P
(P < 0.001 each). PEEP
Δ
P
and PEEPPoeso improved gas-exchange, but PEEPPoeso resulted in lower cardiac output compared with PEEP5 and PEEP
Δ
P
.

CONCLUSION
PEEP
Δ
P
ameliorated the effects of Trendelenburg position with pneumoperitoneum during surgery on end-expiratory P
TP and lung volume, decreased driving pressure and dynamic elastic power, as well as improved gas-exchange while preserving cardiac output.

TRIAL REGISTRATION
German Clinical Trials Register (DRKS00028559, date of registration 2022/04/27). https://drks.de/search/en/trial/DRKS00028559
Alpha-lipoic Acid Protects Against Chronic Alcohol Consumption-induced Cardiac Damage by the Aldehyde Dehydrogenase 2–associated PINK/Parkin Pathway
Authors: Shen Cheng , Chen Xueheng , Cao Yong , Du Yanyan , Xu Xuan , Wu Qingjing , Lin Lizhi , Qin Yiran , Meng Runqi , Gan Lijun , Zhang Jinguo 
Published: September 2023

Abstract
Chronic alcohol intake contributes to high mortality rates due to ethanol-induced cardiac hypertrophy and contractile dysfunction, which are accompanied by increased oxidative stress and disrupted mitophagy. Alpha-lipoic acid (α-LA), a well-known antioxidant, has been shown to protect against cardiac hypertrophy and inflammation. However, little is known about its role and mechanism in the treatment of alcoholic cardiomyopathy. Here, we evaluated the role of α-LA in alcohol-induced cardiac damage by feeding mice a 4.8% (v/v) alcohol diet with or without α-LA for 6 w. Our results suggested that chronic alcohol consumption increased mortality, blood alcohol concentrations, and serum aldehyde levels, but a-LA attenuated the elevations in mortality and aldehydes. Chronic alcohol intake also induced cardiac dysfunction, including enlarged left ventricles, reduced left ventricular ejection fraction, enhanced cardiomyocyte size, and increased serum levels of brain natriuretic peptide, lactate dehydrogenase, and creatine kinase myocardial isoenzyme. Moreover, alcohol intake led to the accumulation of collagen fiber and mitochondrial dysfunction, the effects of which were alleviated by α-LA. In addition, α-LA intake also prevented the increase in reactive oxygen species production and the decrease in mitochondrial number that were observed after alcohol consumption. Chronic alcohol exposure activated PINK1/Parkin-mediated mitophagy. These effects were diminished by α-LA intake by the activation of aldehyde dehydrogenase 2. Our data indicated that α-LA helps protect cardiac cells against the effects of chronic alcohol intake, likely by inhibiting PINK1/Parkin-related mitophagy through the activation of aldehyde dehydrogenase 2.
The S100 calcium-binding protein A6 plays a crucial role in hepatic steatosis by mediating lipophagy
Authors: Du Qian , Zhu Tingting , Wen Guorong , Jin Hai , An Jiaxing , Xu Jingyu , Xie Rui , Zhu Jiaxing , Yang Xiaoxu , Zhang Ting , Liu Qi , Yao Shun , Yang Xingyue , Tuo Biguang , Ma Xiong 
Published: September 2023

Background:
S100 calcium-binding protein A6 (S100A6) is a calcium-binding protein that is involved in a variety of cellular processes, such as proliferation, apoptosis, and the cellular response to various stress stimuli. However, its role in NAFLD and associated metabolic diseases remains uncertain.

Methods and Results:
In this study, we revealed a new function and mechanism of S100A6 in NAFLD. S100A6 expression was upregulated in human and mouse livers with hepatic steatosis, and the depletion of hepatic S100A6 remarkably inhibited lipid accumulation, insulin resistance, inflammation, and obesity in a high-fat, high-cholesterol (HFHC) diet-induced murine hepatic steatosis model. In vitro mechanistic investigations showed that the depletion of S100A6 in hepatocytes restored lipophagy, suggesting S100A6 inhibition could alleviate HFHC-induced NAFLD. Moreover, S100A6 liver-specific ablation mediated by AAV9 alleviated NAFLD in obese mice.

Conclusions:
Our study demonstrates that S100A6 functions as a positive regulator of NAFLD, targeting the S100A6-lipophagy axis may be a promising treatment option for NAFLD and associated metabolic diseases.