bluuebunny/crossref_metadata_2025_split huggingface dataset:
Antioxidant and phytometabolite profiles of ethanolic extract from the cascara pulp of Coffea arabica collected from Gayo Highland: A study for potential anti-photoaging agent
Authors: Lestari Wahyu , Hasballah Kartini , Listiawan M. Yulianto , Sofia Sofia 
Published: September 2023

Background: As the most abundant coffee by-product, cascara pulp has been considered a good source of antioxidants which could be used to prevent photoaging. The aim of this study was to determine the phytometabolite profiles, antioxidant and photoaging properties of the ethanolic extract of Coffea arabica cascara pulp. Methods: Ethanolic maceration was performed on the fine powder of C. arabica cascara pulp collected from Gayo Highland, Aceh Province, Indonesia. The filtrate obtained was evaluated for its 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging activity, total phenolic content (TPC), and total flavonoid content (TFC). The phytometabolite profiling was conducted qualitatively using reagents and quantitatively using gas chromatography—mass spectroscopy (GC-MS). The potential of the cascara pulp phytometabolites in inhibiting activator protein-1 (AP-1) was evaluated through molecular docking. Results: The extract had TPC and TFC of 2.04 mg gallic acid equivalent/g extract and 91.81 mg quercetin equivalent/g extract, respectively. The half-maximal inhibitory concentration (IC50) for the DPPH inhibition reached as low as 9.59 mg/L. Qualitative phytocompound screening revealed the presence of alkaloids, saponins, tannins, flavonoids, steroids, quinones, polyphenols, and triterpenoids. GC-MS revealed the extract containing 5-hydroxy-methylfurfural (22.31%); 2,5-dimethyl-4-hydroxy-3(2H)-furanone (0.74%); and caffeine (21.07%), which could form interaction with AP-1 with binding energies of -172.8, -150.8, and -63.188 kJ/mol, respectively. Conclusion: Ethanolic extract from C. arabica cascara pulp potentially have anti-photoaging properties which is worthy for further investigations in the future.
Immunoinformatics design of B-cell multi-epitope peptide for the diagnosis of Methicillin Resistant Staphylococcus aureus (MRSA) infection
Authors: Olatunde Simeon Kayowa , Owolabi Joshua Babatunde , Ayinde Bolaji Oluwabusayo , Ebanks Shauna Angella , Vidale Edla  Sophia , Oladipo Elijah Kolawole , Alli Yakubu Adekunle , Adewara Enoch Oluwalonimi 
Published: September 2023

Background: Methicillin (oxacillin)-resistant Staphylococcus aureus (MRSA) remains a significant clinical and epidemiological pathogen in hospital settings and in the community world-wide. The resistance to methicillin in Staphylococcus aureus is mediated by the mecA gene, which encodes penicillin-binding protein 2a (PBP2A). Rapid patient screening for MRSA is essential for infection control procedures in order to possibly enhance the outcomes of infected patients. In this study, we utilized PBP2A to predict and create a novel synthetic protein with multiple immunodominant B cell epitopes for rapid diagnosis of MRSA using an in-silico approach. Methods: Seven putative PBP2A peptides were used to analyze the protein’s primary, secondary, and tertiary structures (BepiPred). The B cell construct was then evaluated using I-TASSER server, and physicochemical properties, and homology modeling of the 3 D structure of the protein were obtained. Results: In silico analyses revealed regions with high immunogenicity. Altogether, 19 consented epitopes were selected for the in-silico succession; three consented epitopes from ALJ10988.1, three from ORN56903.1, three from AFJ06714.1, one from AEO00772.1, three from WP_000721309.1, three from WP_057521704.1, and three from WP_063851348.1. The constructs have an average length of 503 amino acids, molecular weight of 55,151.78, instability index of 41.44, theoretical PI of 9.28 and a C score −1.50. In addition, the parameters that were examined indicated the newly multi-epitope construct could potentially serve as a theoretical framework for the development of a MRSA diagnostic kit. Conclusions: Overall, we have developed an antigen-based multi-epitope peptide for the rapid and accurate diagnosis of MRSA infection through an in-silico approach, acceptable in terms of antigenicity, physicochemical properties, structural stability and strong immunogenicity.
Cultural differences in the measurement of self-curiosity within Mexico: a person-centered and variable-centered study
Authors: Aschieri Filippo , Quiroga Garza Angélica , Pascarella Giulia , Zambelli Michela , Tagliabue Semira 
Published: September 2023

Background: This study assessed the role of culture in the measurement of the Self-Curiosity Attitude-Interest scale (SCAI-M), a measure of attitude and interest in increasing one’s knowledge of self, adopting both a person-centered and variable-centered approach. Methods: The study was conducted on a Mexican sample composed of 484 adult participants who completed both the SCAI-M and a series of instruments that measure cultural dimensions through Qualtrix. Data were collected between November 9, to December 18, 2020, and respondents were contacted using advertisements on social media platforms (Facebook and WhatsApp). Analyses included multigroup confirmatory factor analysis and latent profile analysis. Results: A latent profile analysis allowed for the generation of three groups featuring distinct cultural orientations that were similar to previously found cultural profiles (Consensus-oriented Egalitarians, Flexible Individualists, and Rules-based Competitors). Multigroup Confirmative Factor Analysis showed partial metric and scalar invariance for the SCAI-M between groups; moreover, we found proofs of convergent validity with other cultural dimensions besides the ones linked with the Hofstede model. Our results indicate that cultural profiles and cultural variables are associated with both the level and meaning of self-curiosity among Mexican citizens. Conclusions: Finally, the discussion includes considerations on self-curiosity divergence among minority cultures and relevant clinical applications; a field for which we propose future research.
Clinical use of autologous cell-based therapies in an evolving regulatory landscape: A survey of patient experiences and perceptions
Authors: Ogbogu Ubaka , Case Nevicia 
Published: September 2023

Background: Clinical treatments involving autologous cell-based therapies (ACBT) remain prevalent despite a lack of scientific backing and an evolving regulatory landscape aimed at assessing their safety and efficacy for clinical adoption. This study seeks to assess patients’ experiences and perceptions of clinical treatments involving ACBT and their knowledge and views of the regulatory context and associated governance issues. Methods: An anonymous online survey of 181 participants who have been treated or are in the process of being treated with ACBT. Recruitment was via social media platforms. Data was collected through Qualtrics and analyzed using SPSS 29 for the quantitative responses and NVivo 1.7.1 for the qualitative responses. Results: Several themes emerged from the data, including the prominent role of healthcare providers throughout the patient journey, informational practices during the clinical encounter, the high prevalence of pay-for-participation trials, patients’ gaps in regulatory knowledge, and patients’ priorities regarding clinical trials and regulation of ACBT. Conclusions: The study makes a novel contribution to the literature by providing the first analysis of patients’ experiences and perceptions of an emerging cell-based therapy within an evolving regulatory landscape. The findings serve as a valuable resource for developing policy, promoting scientific rigor, and ensuring ethical oversight of ACBT and other upcoming cell-based therapies.
How do we measure the costs, benefits, and harms of sharing data from biomedical studies? A scoping review
Authors: Maxwell Lauren , Shreedhar Priya , Krishnan Ankur 
Published: September 2023

Introduction The benefits of sharing participant-level data from biomedical studies have been widely touted and may be taken for granted. As investments in data sharing and reuse efforts continue to grow, understanding the cost and positive and negative effects of data sharing for research participants, the general public, individual researchers, research and development, clinical practice, and public health is of growing importance. In this scoping review, we will identify and summarize existing evidence on the positive and negative impacts and costs of data sharing and how they are measured. Methods and analysis Eligible studies will report on qualitative or quantitative approaches for measuring the cost of data sharing or its impact on participant privacy, individual or public health, researcher’s careers, clinical or public health practice, or research or development. The systematic search strategy uses MeSH and text terms and is tailored for Ovid Medline, Cumulative Index to Nursing and Allied Health Literature, and Web of Science. We will apply the Arskey and O’Malley scoping review methodology. We selected a scoping rather than a systematic review approach to address multiple related questions and provide guidance related to an emerging field. Two reviewers will conduct the title-abstract and full-text screening and data charting independently. Discrepancies will be resolved through consensus and results will be summarized in a narrative form. Conclusion Research participants, investigators, regulatory groups, ethics review committees, data protection officers, and funders cannot make informed decisions or policies about data reuse without appropriate means of measuring the effects, positive or negative, and cost of data sharing.
Addressing disparities in European Social Sciences & Humanities research on climate, energy and mobility: insights from a Call for Evidence survey and analysis workshops on the challenges and opportunities of working in Southern and Central & Eastern Europe
Authors: Foulds Chris , Crowther Ami , Sorman Alevgul H. , Cabello Violeta , Bálint Dóra , Tagai Gergely , Varjú Viktor , Robison Rosie , Sánchez Ester Galende , Zindulková Kristina 
Published: September 2023

Despite the efforts of the EU, disparities remain in terms of the participation of Social Sciences and Humanities (SSH) researchers from both Southern and Central & Eastern Europe in research collaborations, as compared to Northern and Western European scholars. To better understand these disparities, the EU Horizon Europe SSH CENTRE project ran a Call for Evidence over December 2022 to March 2023. Specifically, respondents were asked about the challenges they faced in conducting SSH research on climate, energy and/or mobility, as well as the ways in which these challenges could be addressed. The Call’s online survey was focused on maximising diversity, and it gathered views and experiences of 137 Southern and Central & Eastern European SSH researchers. The sample was balanced across genders (71 men, 66 women) and the three main themes (82 energy, 88 climate, 53 mobility), and included at least one respondent from each of the 27 target countries. The highest numbers of respondents were from Hungary (19) and Spain (21). To ensure that interpretation and analysis of the data was grounded in regional contexts, we ran two parallel analysis workshops hosted in a hybrid format (combining online and in-person participants): one in Pécs for Central & Eastern European SSH researchers (34 participants); and one in Bilbao for Southern European SSH researchers (26 participants). These workshops focused on discussing the relationship between SSH-STEM disciplines, analysing the institutional contexts, and discussing the implications for domestic and EU research funding relations. During the workshops, data collected through the survey was collectively analysed and the most important reflections were gathered into a common structure of ‘Challenges’ and ‘Ways forward’. Key messages from the workshop are being distilled in a Position Statement that focuses on the common elements while also emphasising possible differences between Southern and Central & Eastern Europe.
Blimp-1 and c-Maf regulate Il10 and negatively regulate common and unique proinflammatory gene networks in IL-12 plus IL-27-driven T helper-1 cells
Authors: Cox Luke S. , Alvarez-Martinez Marisol , Wu Xuemei , Gabryšová Leona , Luisier Raphaëlle , Briscoe James , Luscombe Nicholas M. , O'Garra Anne 
Published: September 2023

Background: CD4+ Th1 cells producing IFN-γ are required to eradicate intracellular pathogens, however if uncontrolled these cells can cause immunopathology. The cytokine IL-10 is produced by multiple immune cells including Th1 cells during infection and regulates the immune response to minimise collateral host damage. In this study we aimed to elucidate the transcriptional network of genes controlling the expression of Il10 and proinflammatory cytokines, including Ifng in Th1 cells. Methods: We applied computational analysis of gene regulation derived from temporal profiling of gene expression clusters obtained from bulk RNA sequencing (RNA-seq) of flow cytometry sorted CD4+ Th1 effector cells differentiated in vitro with IL-12 and IL-27 to produce Ifng and Il10, compared to control driven-CD4+ T cells. Data were integrated with analysis of active genomic regions from these Th1 cells using an assay for transposase-accessible chromatin with sequencing (ATAC)-seq, integrated with literature derived-Chromatin-immunoprecipitation (ChIP)-seq data and the RNA-seq data, to elucidate the transcriptional network of genes controlling expression of Il10 and pro-inflammatory effector genes in Th1 cells. The co-dominant role for the transcription factors, Prdm1 (encoding Blimp-1) and Maf (encoding c-Maf), in cytokine gene regulation in Th1 cells, was confirmed using T cells obtained from mice with T-cell specific deletion of these transcription factors. Results: We show that the transcription factors Blimp-1 and c-Maf each have unique and common effects on cytokine gene regulation and not only co-operate to induce Il10 gene expression in IL-12 plus IL-27 differentiated Th1 cells, but additionally directly negatively regulate key proinflammatory cytokines including Ifng, thus providing mechanisms for reinforcement of regulated Th1 cell responses. Conclusions: These data show that Blimp-1 and c-Maf positively and negatively regulate a network of both unique and common anti-inflammatory and pro-inflammatory genes to reinforce a Th1 response that will eradicate pathogens with minimum immunopathology.
IMMERSIVE JOURNALISM EXPERIENCE IN THE RUSSIAN MEDIA
Authors: Gavrilova Anna A. , Yakovleva Marina G. 
Published: September 2023

The purpose of the article is to study the experience of the Russian media in the field of creating immersive content. The empirical basis of the study was 216 immersive materials of the Russia Today TV channel and 26 immersive materials of RIA Novosti. The study was implemented by the content analysis method. The technique of expert interviews was also used to collect information about the activities of the Directorate of New Media of MIA “Russia Today”.
Results. The article analyzes Russian media experience, i.e., Russia Today channel and RIA Novosti, in using of virtual reality and augmented reality tools and 360° video technology; number, timing, genre and theme specifics and their popularity among the audience are determined; primary issues of Russian media in immersive content production are identified.
The results obtained will be useful in researching trends in the development of journalism, and may also be useful for media editorial offices interested in developing new media product formats and attracting new audience groups.
Stabilized single-frequency sub-kHz linewidth Brillouin fiber laser cavity operating at 1  µm
Authors: Deroh Moise , Lucas Erwan , Hammani Kamal , Millot Guy , Kibler Bertrand 
Published: September 2023

We experimentally demonstrate a stabilized single-frequency Brillouin fiber laser operating at 1.06 µm by means of a passive highly nonlinear fiber (HNLF) ring cavity combined with a phase-locking loop scheme. The stimulated Brillouin scattering efficiency is first investigated in distinct single-mode germanosilicate core fibers with increasing GeO2 content. The most suitable fiber, namely, 21 mol.% GeO2 core fiber, is used as the Brillouin gain medium in the laser cavity made with a 15-m-long segment. A Stokes lasing threshold of 140 mW is reported. We also show significant linewidth narrowing (below 1 kHz) as well as frequency noise reduction compared to that of the initial pump in our mode-hop free Brillouin fiber laser.
Three-dimensional super-resolution range-gated imaging based on Gaussian-range-intensity model
Authors: Wang Pengfei , Liu Hao , Qiu Shaoping , Liu Yu , Huang Feng 
Published: September 2023

Current range-gated imaging methods based on triangular or trapezoidal models face significant limitations in terms of the accuracy and precision of three-dimensional (3D) reconstruction. Here, we establish a range-gated 3D super-resolution image reconstruction method based on a Gaussian-range-intensity model. A denoised optimization method for image preprocessing is established to improve the distance accuracy of the reconstruction. The experimental results show that the model can reduce the error of target distance by 64% and possess the reconstruction of multiple kinds of targets with a distance resolution of 1.5 cm and lower noise, realizing better reconstruction results compared with the existing range-gated 3D imaging techniques.
Six-telescope integrated optics beam combiner fabricated using ultrafast laser inscription for J- and H-band astronomy
Authors: Dinkelaker Aline N. , Smarzyk Sebastian , Nayak Abani S. , Piacentini Simone , Corrielli Giacomo , Osellame Roberto , Pedretti Ettore , Roth Martin M. , Madhav Kalaga 
Published: September 2023

We have built and characterized, to our knowledge, the first six-telescope discrete
beam combiner (DBC) for stellar interferometry in the astronomical
J-band. It is the DBC with the largest number of beam combinations and
was manufactured using ultrafast laser inscription in borosilicate
glass, with a throughput of ≈56%. For calibration of the
visibility-to-pixel matrix, we use a two-input Michelson
interferometer and extract the complex visibility. A visibility
amplitude of 1.05 and relative precision of 2.9% and 3.8% are
extracted for 1328 nm and 1380 nm, respectively.
Broadband (≤40nm) characterization is affected by
dispersion but shows similar performance.
Optical pumping enhancement of a free-induction-decay magnetometer
Authors: Hunter Dominic , Mrozowski Marcin S. , McWilliam Allan , Ingleby Stuart J. , Dyer Terry E. , Griffin Paul F. , Riis Erling 
Published: September 2023

Spin preparation prior to a free-induction-decay (FID) measurement can be adversely affected by transverse bias fields, particularly in the geophysical field range. A strategy that enhances the spin polarization accumulated before readout is demonstrated, by synchronizing optical pumping with a magnetic field pulse that supersedes any transverse fields by over two orders of magnitude. The pulsed magnetic field is generated along the optical pumping axis using a compact electromagnetic coil pair encompassing a micro-electromechanical systems (MEMS) vapor cell. The coils also resistively heat the cesium vapor to the optimal atomic density without spurious magnetic field contributions as they are rapidly demagnetized to approximately zero field during spin readout. The demagnetization process is analyzed electronically, and directly with a FID measurement, to confirm that the residual magnetic field is minimal during detection. The sensitivity performance of this technique is compared to existing optical pumping modalities across a wide magnetic field range. A noise floor sensitivity of 238fT/Hz was achieved in a field of approximately 50 µT, in close agreement with the Cramér–Rao lower bound predicted noise density of 258fT/Hz.
Safety and tolerability of moxidectin and ivermectin combination treatments for lymphatic filariasis in Côte d’Ivoire: A randomized controlled superiority study
Authors: Bjerum Catherine M. , Koudou Benjamin G. , Ouattara Allassane F. , Lew Daphne , Goss Charles W. , Gabo Pascal T. , King Christopher L. , Fischer Peter U. , Weil Gary J. , Budge Philip J. 
Published: September 2023

Background
Moxidectin is a macrocyclic lactone registered for the treatment of human onchocerciasis. The drug has a good safety profile, large volume of distribution and a long elimination half-life. This paper reports tolerability data from the first use of moxidectin in persons with Wuchereria bancrofti infection.

Methods
In this randomized, open-label, masked-observer superiority trial, adults with Wuchereria bancrofti microfilaremia in Côte d’Ivoire were randomized to 1 of 4 treatment arms: ivermectin + albendazole (IA), moxidectin + albendazole (MoxA), ivermectin + diethylcarbamazine (DEC) + albendazole (IDA), or moxidectin + DEC + albendazole (MoxDA). As part of a larger efficacy trial, all participants were closely monitored for 7 days after treatment.

Results
One hundred sixty-four individuals were treated, and monitored for treatment emergent adverse events (TEAE). Eighty-seven participants (53%) experienced one or more mild (grade 1) or moderate (grade 2) TEAE. Four participants had transient Grade 3 hematuria after treatment (3 after IDA and 1 after IA). There were no serious adverse events. There were no significant differences in frequency or types of TEAE between treatment groups (IA = 22/41 (53%), MoxA = 24/40 (60%), IDA = 18/41 (44%), MoxDA = 15/42 (36%), p = 0.530). Fifty-nine participants (36%) had multiple TEAE, and 8.5% had a one or more grade 2 (moderate) TEAE. Grade 2 TEAE were more frequent after triple drug treatments (IDA, 14.6%; MoxDA, 9.5%) than after two-drug treatments (IA, 7.3%; MoxA, 2.5%). There was no difference in TEAEs based on baseline Mf counts (OR 0.69 (0.33, 1.43), p-value 0.319).

Conclusion
All treatment regimens were well tolerated. We observed no difference in safety parameters between regimens that contained ivermectin or moxidectin.

Trial registration
Clinicaltrials.gov, NCT04410406.
Specific and sensitive loop-mediated isothermal amplification (LAMP) method for Madurella strains, eumycetoma filamentous fungi causative agent
Authors: Yoshioka Isato , Mori Yugo , Fahal Ahmed Hassan , Siddig Emmanuel Edwar , Kaneko Satoshi , Yaguchi Takashi 
Published: September 2023

Background
Filamentous fungi of the genus Madurella are the primary causative agents of mycetoma, a disease observed in tropical and subtropical regions. Since early diagnostics based on a morphological approach are difficult and have many shortcomings, a molecular diagnostic method suitable for rural settings is required. In this study, we developed the loop-mediated isothermal amplification (LAMP) method to present a foundational technique of the diagnosis of Madurella spp. (M. mycetomatis, M. pseudomycetomatis, M. tropicana, and M. fahalii), the common causative organisms of eumycetoma.

Principal findings
We successfully designed a primer pair targeting the rDNAs of three Madurella spp. excluding M. fahalii, and detected up to 100 fg of genomic DNA extracted from isolates of M. mycetomatis and 1 pg of M. pseudomycetomatis and M. tropicana, within one hour. Second, a primer pair specific to M. mycetomatis, the most common causative species, or M. fahalii, a drug-resistant species, was constructed, and the detection limit of both primer pairs was 1 pg. The designed primers accurately distinguished 16 strains of the genus Madurella from various fungal species known to cause mycetomas.

Conclusion
In summary, we established the first model of a LAMP detection method that rapidly and sensitively detects and identifies Madurella isolates for clinical diagnostics. Moreover, the combined designed primer sets could identify mycetoma-causing strains simultaneously.