bluuebunny/crossref_metadata_2025_split huggingface dataset:
Long-term tracking of social structure in groups of rats
Authors: Nagy Máté , Davidson Jacob D. , Vásárhelyi Gábor , Ábel Dániel , Kubinyi Enikő , El Hady Ahmed , Vicsek Tamás 
Published: March 2023

ABSTRACTRodents serve as an important model for examining both individual and collective behavior. Dominance within rodent social structures can determine access to critical resources, such as food and mating opportunities. Yet, many aspects of the intricate interplay between individual behaviors and the resulting group social hierarchy, especially its evolution over time, remain unexplored. In this study, we utilized an automated tracking system that continuously monitored groups of male rats for over 250 days to enable an in-depth analysis of individual behavior and the overarching group dynamic. We describe the evolution of social structures within a group and additionally investigate how past behaviors influence the emergence of new social hierarchies when group composition and experimental area changes. Notably, we find that conventional individual and pairwise tests exhibit a weak correlation with group behavior, highlighting their limited accuracy in predicting behavioral outcomes in a collective context. These results emphasize the context-dependence of social behavior as an emergent property of interactions within a group and highlight the need to measure and quantify social behavior in more naturalistic environments.
Neuron cilia constrain glial regulators to microdomains around distal neurons
Authors: Ray Sneha , Gurung Pralaksha , Manning R. Sean , Kravchuk Alexandra , Singhvi Aakanksha 
Published: March 2023

ABSTRACTEach glia interacts with multiple neurons, but the fundamental logic of whether it interacts with all equally remains unclear. We find that a single sense-organ glia modulates different contacting neurons distinctly. To do so, it partitions regulatory cues into molecular microdomains at specific neuron contact-sites, at its delimited apical membrane. For one glial cue, K/Cl transporter KCC-3, microdomain-localization occurs through a two-step, neuron-dependent process. First, KCC-3 shuttles to glial apical membranes. Second, some contacting neuron cilia repel it, rendering it microdomain-localized around one distal neuron-ending. KCC-3 localization tracks animal aging, and while apical localization is sufficient for contacting neuron function, microdomain-restriction is required for distal neuron properties. Finally, we find the glia regulates its microdomains largely independently. Together, this uncovers that glia modulate cross-modal sensor processing by compartmentalizing regulatory cues into microdomains. Glia across species contact multiple neurons and localize disease-relevant cues like KCC-3. Thus, analogous compartmentalization may broadly drive how glia regulate information processing across neural circuits.
Speed-independent modulation of locomotor gait preference by sensory feedback in mice
Authors: Mitrevica Zane , Murray Andrew J 
Published: March 2023

SummaryLocomotion is one of the most ubiquitous motor actions in the animal kingdom, essential for behaviours as diverse as foraging, migration, and escape. Successful execution of all these tasks relies on continual adjustment of locomotor gait in line with the behavioural demand for speed as well as the terrain. Failure in this process would disrupt locomotor smoothness, raise its energetic cost, and increase the risk of injury due to skeletal stress [1, 2]. Animals avoid these scenarios, in part, by transitioning from left-right alternating (walk, trot) to synchronous (gallop, bound) gaits as they increase the speed [3, 4]. However, this relationship is not deterministic [5, 6] and its connection to biomechanical factors, like the loading of limbs [7, 8], is unclear. To address this, we developed a head-fixed locomotor paradigm that decouples the speed- and leg loading-related influences on gait by combining optogenetic stimulation of an established speed-control pathway [9, 10] with head height or surface incline modulation. We found a pronounced speed-independent shift in homolateral limb coordination from strict alternation to a gallop-like pattern at upward oriented body postures and upsloping terrains. Both conditions are associated with greater relative loading of the hindlimbs and have a consistent effect on gait preference during head-fixed and head-free locomotion. These results suggest that mice use proprioceptive feedback from the limbs to coordinate their gait across speeds and environments, and implicate ipsilateral control mechanisms in this process. More broadly, our work serves as a principled entry point to a behaviour-driven study of gait circuits.
Predicting an Individual’s Cerebellar Activity from Functional Connectivity Fingerprints
Authors: Tripathi Vaibhav , Somers David C 
Published: March 2023

AbstractThe cerebellum is gaining scientific attention as a key neural substrate of cognitive function; however, individual differences in the cerebellar organization have not yet been well studied. Individual differences in functional brain organization can be closely tied to individual differences in brain connectivity. ‘Connectome Fingerprinting’ is a modeling approach that predicts an individual’s brain activity from their connectome. Here, we extend ‘Connectome Fingerprinting’ (CF) to the cerebellum. We examined functional MRI data from 160 subjects (98 females) of the Human Connectome Project young adult dataset. For each of seven cognitive task paradigms, we constructed CF models from task activation maps and resting-state cortico-cerebellar functional connectomes, using a set of training subjects. For each model, we then predicted task activation in novel individual subjects, using their resting-state functional connectomes. In each cognitive paradigm, the CF models predicted individual subject cerebellar activity patterns with significantly greater precision than did predictions from the group average task activation. Examination of the CF models revealed that the cortico-cerebellar connections that carried the most information were those made with the non-motor portions of the cerebral cortex. These results demonstrate that the fine-scale functional connectivity between the cerebral cortex and cerebellum carries important information about individual differences in cerebellar functional organization. Additionally, CF modeling may be useful in the examination of patients with cerebellar dysfunction, since model predictions require only resting-state fMRI data which is more easily obtained than task fMRI.
Universal open MHC-I molecules for rapid peptide loading and enhanced complex stability across HLA allotypes
Authors: Sun Yi , Young Michael C. , Woodward Claire H. , Danon Julia N. , Truong Hau , Gupta Sagar , Winters Trenton J. , Burslem George , Sgourakis Nikolaos G. 
Published: March 2023

AbstractThe polymorphic nature and intrinsic instability of class I major histocompatibility complex (MHC-I) and MHC-like molecules loaded with suboptimal peptides, metabolites, or glycolipids presents a fundamental challenge for identifying disease-relevant antigens and antigen-specific T cell receptors (TCRs), hindering the development of autologous therapeutics. Here, we leverage the positive allosteric coupling between the peptide and light chain (β2microglobulin, β2m) subunits for binding to the MHC-I heavy chain (HC) through an engineered disulfide bond bridging conserved epitopes across the HC/β2m interface, to generate conformationally stable, open MHC-I molecules. Biophysical characterization shows that open MHC-I molecules are properly folded protein complexes of enhanced thermal stability compared to the wild type, when loaded with low- to intermediate-affinity peptides. Using solution NMR, we characterize the effects of the disulfide bond on the conformation and dynamics of the MHC-I structure, ranging from local changes in β2m interacting sites of the peptide binding groove to long-range effects on the α2-1helix and α3domain. The interchain disulfide bond stabilizes empty MHC-I molecules in a peptide-receptive, open conformation to promote peptide exchange across multiple human leucocyte antigen (HLA) allotypes, covering representatives from five HLA-A, six HLA-B supertypes, and oligomorphic HLA-Ib molecules. Our structural design, combined with conditional β-peptide ligands, provides a universal platform for generating ready-to-load MHC-I systems of enhanced stability, enabling a range of approaches to screen antigenic epitope libraries and probe polyclonal TCR repertoires in the context of highly polymorphic HLA-I allotypes, as well as oligomorphic nonclassical molecules.Significance StatementWe outline a structure-guided approach for generating conformationally stable, open MHC-I molecules with enhanced ligand exchange kinetics spanning five HLA-A, all HLA-B supertypes, and oligomorphic HLA-Ib allotypes. We present direct evidence of positive allosteric cooperativity between peptide binding and β2m association with the heavy chain by solution NMR and HDX-MS spectroscopy. We demonstrate that covalently linked β2m serves as a conformational chaperone to stabilize empty MHC-I molecules in a peptide-receptive state, by inducing an open conformation and preventing intrinsically unstable heterodimers from irreversible aggregation. Our study provides structural and biophysical insights into the conformational properties of MHC-I ternary complexes, which can be further applied to improve the design of ultra-stable, universal ligand exchange systems in a pan-HLA allelic setting.
Diverse and abundant phages exploit conjugative plasmids
Authors: Quinones-Olvera Natalia , Owen Siân V. , McCully Lucy M. , Marin Maximillian G. , Rand Eleanor A. , Fan Alice C. , Martins Dosumu Oluremi J. , Paul Kay , Sanchez Castaño Cleotilde E. , Petherbridge Rachel , Paull Jillian S. , Baym Michael 
Published: March 2023

SummaryPhages exert profound evolutionary pressure on bacteria by interacting with receptors on the cell surface to initiate infection. While the majority of phages use chromosomally-encoded cell surface structures as receptors, plasmid-dependent phages exploit plasmid-encoded conjugation proteins, making their host range dependent on horizontal transfer of the plasmid. Despite their unique biology and biotechnological significance, only a small number of plasmid-dependent phages have been characterized. Here we systematically search for new plasmid-dependent phages targeting IncP and IncF plasmids using a targeted discovery platform, and find that they are common and abundant in wastewater, and largely unexplored in terms of their genetic diversity. Plasmid-dependent phages are enriched in non-canonical types of phages, and all but one of the 64 phages we isolated were non-tailed, and members of the lipid-containing tectiviruses, ssDNA filamentous phages or ssRNA phages. We show that plasmid-dependent tectiviruses exhibit profound differences in their host range which is associated with variation in the phage holin protein. Despite their relatively high abundance in wastewater, plasmid-dependent tectiviruses are missed by metaviromic analyses, underscoring the continued importance of culture-based phage discovery. Finally, we identify a tailed phage dependent on the IncF plasmid, and find related structural genes in phages that use the orthogonal type 4 pilus as a receptor, highlighting the promiscuous use of these distinct contractile structures by multiple groups of phages. Taken together, these results indicate plasmid-dependent phages play an under-appreciated evolutionary role in constraining horizontal gene transfer via conjugative plasmids.
Monetization of politics and public procurement in Ghana
Authors: Lassou Philippe Jacques Codjo , Sorola Matthew , Senkl Daniela , Lauwo Sarah George , Masse Chelsea 
Published: March 2023

PurposeThis paper aims to investigate the prevalence of corruption in Ghana to understand how and why it has turned public procurement into a mere money-making scheme instead of a means to provide needed public goods and services.Design/methodology/approachThe study focuses on Ghana as a case study and mobilizes the monetization of politics lenses. Data are collected via interviews with key officials across the procurement sector (including the government, donors and civil society), documents, documentaries and news articles.FindingsThe findings suggest that the increasing costs of elections and political financing coupled with the costs of vote-buying, which has become informally institutionalized, intensify corruption practices and, consequently, turns public procurement into a mere source of cash for political ends. Political appointments and legalized loopholes facilitate this by helping to nullify the safeguard accounting and other control institutions are designed to provide. Likewise, enduring poverty and rising inequality “force” citizens into a vote-buying culture which distorts democratic premises that may drive out unscrupulous politicians; thus, perpetuating capture schemes. Civil society's efforts to remedy these have had little success, and corruption and inequality remain rife.Practical implicationsThe main practical implication of the study lies in the need for a gradual demonetization of elections, and the consideration of the fundamental function of public procurement as a policy instrument embedded in economic, social, cultural and environmental plans. Additionally, given the connectedness of the various corruption issues raised, a comprehensive system-based approach in dealing with them would be more effective than a piecemeal approach targeting each issue/problem in isolation.Originality/valueWhile extant literature has examined the issue of endemic corruption in developing countries using state capture, few have attempted to explain why it remains enduring, particularly in public procurement. This study, therefore, contributes to the literature on corruption and state capture theoretically and empirically by drawing on monetization of politics from political science to explain why corruption and state capture endure in certain contexts (with Ghana as an illustrative example) which reduce public procurement to a cash-milking scheme.
Interconnectedness and the web of accountabilities: Humboldtian approaches to social and environmental accounting
Authors: Lehner Othmar Manfred , Kyriacou Orthodoxia 
Published: March 2023

PurposeCurrent accounting practice tends to split environmental complexities into quantifiable, codified elements, producing codified simplifications of the “complex” in pursuit of environmental externalities. This has led to standardization, but has done little to motivate organizations to engage in more environmentally-aware behavior that transcends the coercive dimensions of codification. The work of Alexander von Humboldt (1769–1859) can bring new insights and perspectives to social and environmental accounting (SEA). In discussing Humboldt's philosophy of understanding the interconnectedness between people, their contexts (cultures) and their environment, the authors contribute to the emerging SEA literature on notions of interconnectedness and the web of accountabilities. The authors also explore how a Humboldtian approach may help break through the current epistemological boundaries of SEA by combining accurate measurement with imagery to make the “complex” manageable whilst embracing interconnectedness and hermeneutics.Design/methodology/approachIn this conceptual paper, the authors humbly draw on Humboldt's legacy and explore the underlying philosophical assumptions of Humboldtian science. The authors then contrast these with current SEA approaches in the literature and derive new insights into their intentionality and practical use.FindingsRe-examining Humboldt's pioneering work enables us to pinpoint what might be missing from current SEA approaches and debates. Humboldt upheld an “ethics of precision,” which included both measurement accuracy and qualitative relevance, and combined hands-on scientific fieldwork with the aesthetic ideals and interconnectedness of the age of Romanticism. Drawing on Humboldtian science, the authors propose focusing on the interconnectedness of nature and humanity, embracing the qualitative and hermeneutical and including aesthetics and emotion in environmental visualizations.Originality/valueThe paper elucidates why and how Humboldtian science might inform, guide and enhance the emancipatory potential of SEA in the 21st century. Specifically, the authors discuss Humboldt's approach of linking accurate measurement with imagery to convey a sense of interconnectedness.
Technological diversification, technological coupling and invention performance
Authors: Martynov Aleksey 
Published: March 2023

PurposeTo investigate the relationship between technological diversification and firm performance as a function of varying levels of technological coupling and internal technological change.Design/methodology/approachA longitudinal study of US-based bio-pharmaceutical companies.FindingsTechnological diversification improves invention performance. However, high levels of technological coupling reduce this effect.Practical implicationsFirms with highly diversified technological portfolios should strive to keep their technologies at low levels of technological coupling.Originality/valueThis is the first study to show that technological coupling reduces the positive effect of technological diversification on firms' invention performance.
Performance measurement indicators influential to the espousal of cyber-physical systems for facilities management – a Delphi approach
Authors: Ikuabe Matthew , Aigbavboa Clinton Ohis , Anumba Chimay , Oke Ayodeji Emmanuel 
Published: March 2023

Purpose
The quest for improved facilities management (FM) delivery is receiving immense focus through the incorporation of innovative technologies such as cyber-physical systems (CPS). The system’s high computational capabilities can aid in the abatement of some of the challenges plaguing FM functions. However, the requisite ingredients for the uptake of the system for FM have still not gained scholarly attention. Because performance measurement is a vital index in determining the outcome of FM methods, this study aims to investigate the influence of performance measurement indicators that are influential to the uptake of CPS for delivering FM functions.

Design/methodology/approach
A qualitative technique was adopted using the Delphi technique. The panel of experts for the study was selected through a well-defined process based on stipulated criteria. The experts gave their opinions in two rounds before consensus was attained on the identified performance measurement indicators, whereas methods of data analysis were measures of central tendency, inter-quartile deviation and Mann–Whitney U test.

Findings
Results from this study showed that 11 of the performance indicators were of very high significance in the determination of the uptake of CPS for FM functions, whereas 5 of the indicators were proven to be of high significance. Furthermore, there was no statistical difference in the opinions of the experts based on their affiliation with academic institutions and professional practice.

Practical implications
The findings of this study contribute practically by aiding policymakers, facility managers and relevant stakeholders with the vital knowledge of delivery mandates for efficient FM services that can spur the uptake of digital technologies such as CPS.

Originality/value
This study contributes to the body of knowledge as it unveils a roadmap of the expected performance output and its accompanying evaluation that would drive the adoption of a promising technology such as CPS in the delivery of FM tasks.
Fault diagnosis of wind turbine power converter using intrinsic mode functions with relative energy entropy
Authors: R. Manikandan , R. Raja Singh 
Published: March 2023

Purpose
The purpose of this paper is to prevent the destruction of other parts of a wind energy conversion system because of faults, the diagnosis of insulated-gate bipolar transistor (IGBT) faults has become an essential topic of study. Demand for sustainable energy sources has been prompted by rising environmental pollution and energy requirements. Renewable energy has been identified as a viable substitute for conventional fossil fuel energy generation. Because of its rapid installation time and adaptable expenditure for construction scale, wind energy has emerged as a great energy resource. Power converter failure is particularly significant for the reliable operation of wind power conversion systems because it not only has a high yearly fault rate but also a prolonged downtime. The power converters will continue to operate even after the failure, especially the open-circuit fault, endangering their other parts and impairing their functionality.

Design/methodology/approach
The most widely used signal processing methods for locating open-switch faults in power devices are the short-time Fourier transform and wavelet transform (WT) – based on time–frequency analysis. To increase their effectiveness, these methods necessitate the intensive use of computational resources. This study suggests a fault detection technique using empirical mode decomposition (EMD) that examines the phase currents from a power inverter. Furthermore, the intrinsic mode function’s relative energy entropy (REE) and simple logical operations are used to locate IGBT open switch failures.

Findings
The presented scheme successfully locates and detects 21 various classes of IGBT faults that could arise in a two-level three-phase voltage source inverter (VSI). To verify the efficacy of the proposed fault diagnosis (FD) scheme, the test is performed under various operating conditions of the power converter and induction motor load. The proposed method outperforms existing FD schemes in the literature in terms of fault coverage and robustness.

Originality/value
This study introduces an EMD–IMF–REE-based FD method for VSIs in wind turbine systems, which enhances the effectiveness and robustness of the FD method.
Development trends and current status of facility management and professionalization of facility services in Turkey
Authors: Keskin Esra , Tanrıvermiş Harun , Temeljotov Salaj Alenka 
Published: March 2023

Purpose
Facility management (FM) is picking up steam in Turkey. However, delays in creating standards and adopting FM as a profession by corporate and public institutions led to an unusual predicament, unlike in Europe and the USA. The purpose of this study is to take an inductive and constructivist approach to explore the extent of maturity in the FM industry within Turkey.

Design/methodology/approach
Qualitative understanding of the complexity of the Turkish FM sector drives the design used in the study. All data has been collected through an in-depth interview and semi-structured questionnaire with FM stakeholders.

Findings
The findings revealed the perceptions of stakeholders working in various businesses and research institutions in Turkey, including FM organizations, supply markets and educational institutions, regarding the key challenges and opportunities in the business environment.

Practical implications
The trend on Turkish FM revealed a slow growth in FM professionalization, which needs the authority’s attention as the professionalization reduces the unemployment rate, reduces cost and enhances the life cycle of the physical property.

Originality/value
This is the first study, to the best of the authors’ knowledge, on Turkish FM that provides comprehensive studies on Turkish FM professionalization trends to draw the attention of policymakers to the need to enact policies that would improve FM professionalism.