bluuebunny/crossref_metadata_2025_split huggingface dataset:
Evolutionary Trajectory of Pattern Recognition Receptors in Plants
Authors: Ngou Bruno Pok Man , Wyler Michele , Schmid Marc W , Kadota Yasuhiro , Shirasu Ken 
Published: July 2023

AbstractPlants perceive pathogen-associated molecular patterns (PAMPs) via pattern recognition receptors (PRRs) to activate PRR-triggered immunity (PTI). Despite extensive research on PTI in model plant species, the evolutionary trajectory and emergence of PRRs remain elusive. Here we conducted a comparative genomic analysis of cell-surface receptors and downstream signalling components among 350 plant species. Our findings reveal that cell-surface receptors comprise two major classes, receptor-like proteins (RLPs) and receptor-like kinases (RLKs), with RLP being more ancient whereas RLK families have undergone significant expansion. We also demonstrate that multiple downstream signalling components have an ancient origin within the plant lineage. To shed light on the immune-specificity of PRRs, we traced the evolutionary origin of immune-specific leucine-rich repeat-RLPs (LRR-RLPs) in plants. Surprisingly, we discovered that the last four LRR motifs crucial for co-receptor interaction in LRR-RLPs are closely related to those of the LRR-RLK subgroup Xb, which primarily governs growth and development. Functional characterisation further reveals that LRR-RLPs initiate immune responses through their juxtamembrane and transmembrane regions, while LRR-RLK-Xb members regulate development through their cytosolic kinase domains. Our data suggest modular evolution of cell-surface receptors in which immunity- and development-specific cell-surface receptors share a common origin. After diversification, their ectodomains, juxtamembrane, transmembrane, and cytosolic regions have either diversified or stabilised to recognize ligands that activate different downstream responses. We propose that cell-surface receptors and downstream signalling components are ancient, and likely predate the emergence of land plants, subsequently evolving to exhibit greater complexity and specificity within the land plant lineage.
Effects of Copy Number Variations on Longevity in Late-Onset Alzheimer’s Disease Patients: Insights from a Causality Network Analysis
Authors: Hao Yanan , Li Chuhao , Wang He , Ming Chen 
Published: July 2023

AbstractAlzheimer’s disease (AD), particularly late-onset Alzheimer’s disease (LOAD), is a prevalent form of dementia that significantly affects patients’ cognitive and behavioral capacities and longevity. Although approximately 70 genetic risk factors linked with AD have been identified, their influence on patient longevity remains unclear. Further, recent studies have associated copy number variations (CNVs) with the longevity of healthy individuals and immune-related pathways in AD patients. This study aims to investigate the role of CNVs on the longevity of AD patients by integrating multi-omics data from the Religious Orders Study/Memory and Aging Project (ROSMAP) cohort through causality network inference.Our comprehensive analysis led to the construction of a CNV-gene-age of death (AOD) causality network. We successfully identified three key CNVs (DEL5006, mCNV14192, and DUP42180) and seven AD-longevity causal genes (PLGRKT, TLR1, PLAU, CALB2, SYTL2, OTOF,andNT5DC1) impacting AD patient longevity, independent of disease severity. This outcome emphasizes the potential role of plasminogen activation and chemotaxis in longevity. We propose several hypotheses regarding the role of identified CNVs and the plasminogen system on patient longevity.However, experimental validation is required to further corroborate these findings and uncover precise mechanisms. Despite these limitations, our study offers promising insights into the genetic influence on AD patient longevity and contributes to paving the way for potential therapeutic interventions.
A neural mechanism for discriminating social threat from social safety
Authors: Kassraian Pegah , Bigler Shivani K. , Gilly Diana M. , Shrotri Neilesh , Siegelbaum Steven A. 
Published: July 2023

AbstractThe ability to distinguish a threatening from non-threatening conspecific based on past experience is critical for adaptive social behaviors. Although recent progress has been made in identifying the neural circuits that contribute to different types of positive and negative social interactions, the neural mechanisms that enable the discrimination of individuals based on past aversive experiences remain unknown. Here, we developed a modified social fear conditioning paradigm that induced in both sexes robust behavioral discrimination of a conspecific associated with a footshock (CS+) from a non-reinforced interaction partner (CS-). Strikingly, chemogenetic or optogenetic silencing of hippocampal CA2 pyramidal neurons, which have been previously implicated in social novelty recognition memory, resulted in generalized avoidance fear behavior towards the equally familiar CS-and CS+. One-photon calcium imaging revealed that the accuracy with which CA2 representations discriminate the CS+ from the CS-animal was enhanced following social fear conditioning and strongly correlated with behavioral discrimination. Moreover the CA2 representations incorporated a generalized or abstract representation of social valence irrespective of conspecific identity and location. Thus, our results demonstrate, for the first time, that the same hippocampal CA2 subregion mediates social memories based on conspecific familiarity and social threat, through the incorporation of a representation of social valence into an initial representation of social identity.
The neural basis of defensive behaviour evolution inPeromyscusmice
Authors: Baier Felix , Reinhard Katja , Tong Victoria , Murmann Julie , Farrow Karl , Hoekstra Hopi E. 
Published: July 2023

ABSTRACTEvading imminent predator threat is critical for survival. Effective defensive strategies can vary, even between closely related species. However, the neural basis of such species-specific behaviours is still poorly understood. Here we find that two sister species of deer mice (genusPeromyscus) show different responses to the same looming stimulus:P. maniculatus,which occupy densely vegetated habitats, predominantly dart to escape, while the open field specialist,P. polionotus,pause their movement. This difference arises from species-specific escape thresholds, is largely context-independent, and can be triggered by both visual and auditory threat stimuli. Using immunohistochemistry and electrophysiological recordings, we find that although visual threat activates the superior colliculus in both species, the role of the dorsal periaqueductal gray (dPAG) in driving behaviour differs. While dPAG activity scales with running speed and involves both excitatory and inhibitory neurons inP. maniculatus, the dPAG is largely silent inP. polionotus,even when darting is triggered. Moreover, optogenetic activation of excitatory dPAG neurons reliably elicits darting behaviour inP. maniculatusbut notP. polionotus. Together, we trace the evolution of species-specific escape thresholds to a central circuit node, downstream of peripheral sensory neurons, localizing an ecologically relevant behavioural difference to a specific region of the complex mammalian brain.
Cryo-EM structure of TMEM63C suggests it functions as a monomer
Authors: Qin Yuqi , Yu Daqi , Wu Dan , Dong Jiangqing , Li William Thomas , Ye Chang , Cheung Kai Chit , Zhang Yingyi , Xu Yun , Wang YongQiang , Shi Yun Stone , Dang Shangyu 
Published: July 2023

AbstractThe TMEM63 family proteins (A, B, and C), calcium-permeable channels in animals that are preferentially activated by hypo-osmolality, have been implicated in various physiological functions. Deficiency of these channels would cause many diseases including hearing loss. However, their structures and physiological roles are not yet well understood. In this study, we determined the cryo-EM structure of the mouse TMEM63C at 3.56 Å, and revealed structural differences compared to its plant orthologues OSCAs. Further structural guided mutagenesis and electrophysiological studies demonstrated the important roles of the coupling of TM0 and TM6 in channel activity. Additionally, we confirmed that the physiological state of TMEM63C is monomer, while TMEM63B is a mix of monomer and dimer in cells, suggesting that oligomerization is a novel regulatory mechanism for TMEM63 proteins.
New record ofDendronephthya hemprichi(Family: Nephtheidae) from Mediterranean, Israel- an evidence for tropicalization?
Authors: Nativ Hagai , Galili Ori , Almuly Ricardo , Einbinder Shai , Tchernov Dan , Mass Tali 
Published: July 2023

AbstractBioinvasions have the potential to provoke cascade effects that can disrupt natural ecosystems, and cause ecological regime shifts. The Mediterranean Sea is particularly prone to bioinvasions as the changing water conditions, evoked by climate change, are creating advantageous conditions for Lessepsian migrants from the Red Sea. Recently, in May 2023, a new alien species was documented in the Mediterranean Sea - a soft coral of the genusDendronephthya. This discovery was made by divers conducting ‘Long Term Ecological Research’ surveys, along the coast of Israel, at a depth of 42 m. Genetic and morphological testing were utilized to confirm the species identity asDendronepthya hemprichi, an Indo-Pacific coral, common in the Red Sea. According to life history traits of this species such as accelerated attachment to available surfaces and fast growth, we expect it to rapidly expand its distribution and abundance across the Mediterranean.HighlightsPotential ‘Tropicalization’ of the Mediterranean SeaIncreasing water temperatures are an important vector for marine bioinvasionA second soft coral species, associated with Lessepsian migration, was identified in the MediterraneanThe importance of a long-term ecological monitoring program for identifying ecosystem changes
Metabolism regulates muscle stem cell self-renewal by connecting the microenvironment and histone acetylation
Authors: Ly C. Hai , Chung Jin D. , Nguyen John H.V. , Tian Luyi , Schroeder Jan , Knaupp Anja S. , Su Shian , Trieu Jennifer , Salmi Talhah M. , Zalcenstein Daniela , Jabbari Jafar S. , Boughton Berin A. , Cox Andrew G. , Naik Shalin H. , Polo Jose M. , Ritchie Matthew E. , Lynch Gordon S. , Ryall James G. 
Published: July 2023

SUMMARYSkeletal muscle contains a resident population of somatic stem cells capable of both self-renewal and differentiation. The signals that regulate this important decision have yet to be fully elucidated. Here we use metabolomics and mass spectrometry imaging (MSI) to identity a state of localized hyperglycaemia following skeletal muscle injury. We show that committed muscle progenitor cells exhibit an enrichment of glycolytic and TCA cycle genes and that extracellular monosaccharide availability regulates intracellular citrate levels and global histone acetylation. Muscle stem cells exposed to a reduced (or altered) monosaccharide environment demonstrate reduced global histone acetylation and transcription of myogenic determination factors (includingmyod1). Importantly, reduced monosaccharide availability was linked directly to increased rates of asymmetric division and muscle stem cell self-renewal in regenerating skeletal muscle. Our results reveal an important role for the extracellular metabolic environment in the decision to undergo self-renewal or myogenic commitment during skeletal muscle regeneration.
MCDM model to rank the performance outcomes in the implementation of Industry 4.0
Authors: Kumar Veepan , Vrat Prem , Shankar Ravi 
Published: July 2023

PurposeIndustry 4.0 has received significant attention in today's competitive business market, necessitating a restructuring of functional domains in nearly every manufacturing organization. A comprehensive strategy to improve performance in preparation for Industry 4.0 implementation necessitates several steps, one of which is the establishment of performance outcomes (POs). The aim of this paper is to identify and rank the POs realized due to the adoption of Industry 4.0 enablers.Design/methodology/approachBased on an extensive literature review and inputs received from experts, a comprehensive list of enablers and the POs was prepared and finalized. This paper proposes a framework based on hybrid solution methodology, namely Neutrosophic Analytical Hierarchy Process (N-AHP) and Neutrosophic Combined Compromise Solution (N-CoCoSo), to rank the POs realized due to the adoption of Industry 4.0 enablers. The N-AHP methodology has been adopted to calculate the relative weights of the Industry 4.0 enablers. In comparison, the N-CoCoSo method has been adopted to rank the POs of Industry 4.0.FindingsThe proposed framework is applied to an Indian manufacturing organization to test the organization's practical applicability. Additionally, sensitivity analysis is also carried out to check the steadiness of the proposed framework. The findings of this study revealed that “Improved responsiveness to market conditions in today's competitive business environment” is the top-ranked PO of Industry 4.0, followed by “Enhanced competitiveness and better market share”, “Better product quality, through smart management of production process” and “Reduction in manufacturing waste and environmental sustainability” which could be realized due to adoption of its enablers.Practical implicationsThis research would aid practitioners by enhancing the practitioners' capacity to understand and prioritize the various POs resulting from implementing Industry 4.0 enablers. Embracing a clear strategic plan will further assist practitioners in improving the efficiency of Industry 4.0 implementation.Originality/valuePrevious literature has only addressed the relationship between Industry 4.0 enablers and POs in a limited way. This paper attempts to compile a comprehensive list of Industry 4.0 enablers relevant to manufacturing organizations in order to fill this knowledge and research gap.
Executive compensation incentives, innovation openness and green innovation: evidence from China's heavily polluting enterprises
Authors: Zhao Jianfei , Chankoson Thitinan , Cheng Wenjin , Pongtornkulpanich Anan 
Published: July 2023

PurposeA green innovation strategy is an important step for enterprises to balance economic and environmental. As the executors of strategic decisions, the attitude and capabilities of senior managers determine the effectiveness of implementing green innovation. Therefore, this paper aims to explore the relationship between executive compensation incentives and green innovation.Design/methodology/approachBased on the data of heavily polluting enterprises listed in China's A-share market from 2015 to 2020, this study constructs an OLS model with fixed effects of time and industry, and uses the mediation three-step method to verify the correlation between executive compensation incentives, innovation openness and green innovation. Meanwhile, the grouping regression was used to test the moderating effect of environmental regulation on executive compensation incentives.FindingsThe empirical results show that executive salary incentives promote green innovation and equity incentives inhibit green innovation; the openness breadth partially mediates the relationship between salary incentives, equity incentives and green innovation, while the openness depth only partially mediates the relationship between equity incentives and green innovation; and environmental regulation positively moderates executive incentives.Research limitations/implicationsDue to sample selection and variable measurement, the study lacks certain generality. Therefore, future research needs to further analyze the internal factors affecting green innovation from multiple dimensions.Practical implicationsThis study provides a new evidence for analyzing how executive compensation measures affect green innovation, and further enhances the mediating mechanism of open innovation.Originality/valueThis study has significant theoretical implications for examining the intra-firm factors that affect green innovation.
Exploring the generational influence on social media-based tourist decision-making in India
Authors: Gulati Shruti 
Published: July 2023

Purpose
This study aims to explore how social media affects decision-making among tourists and whether there is a potential effect of age, which is studied through generations. For this purpose, baby boomers, Gen X, Gen Y and Gen Z tourists are studied and real-time implications are offered.

Design/methodology/approach
The study adopts a descriptive and exploratory design where the conceptual model of social media-based decision-making is developed through a review of the literature. Quantitative analysis is conducted on primary data from 600 Indian tourists. This is done using a self-administered questionnaire adopted from Gulati (2022) after checking its validity and reliability. The statistical analysis for hypothesis testing is done using PLS-SEM path modelling on pooled data. To study the categorical moderating effect of generations, partial least squares multigroup analysis (PLS-MGA) is performed as a paired comparison on every successive generation.

Findings
After testing every successive younger generation with an older generation through PLS-MGA, none of the pairs found any significant differences in path coefficients, as the values obtained were 0.05 < p < 0.95 for all five paths (SM → NR, SM → IS, SM → E, SM → P, SM → PPB). This indicates all the generations behave in a similar manner irrespective of them being older or younger, and age does not moderate social media’s impact on decision-making among Indian tourists.

Research limitations/implications
The study establishes India as a unique geographical market and suggests tourism marketers to treat all generations at par, irrespective of age, as they behave and interact with social media in a similar manner. But, because this study is restricted to a single geographical location, i.e. India, further regions can be explored for global generalisation. Future research can also explore other demographics for combined, moderated analysis. Findings from the study suggest that marketers should ensure that equal attention is given to all generations as they engage with social media in a similar manner. Targeted marketing using artificial intelligence can help in ensuring custom ads. Personalisation according to generations can also facilitate greater purchases.

Originality/value
The study fills a major population and knowledge gap by exploring a topic that has been highly under-researched. Also, the study adopts an inclusive approach by analysing all the generations, both younger and older, to understand the potential effect of age on moderating the impact that social media has on tourist decision-making. Further, real-time suggestions and implications are offered to tourism marketers with special reference to the Indian tourism industry.
Success comes before work only in dictionary: role of job autonomy for intrapreneurial behaviour using trait activation theory
Authors: Alam Muhammad Zubair , Rafiq Muhammad , Alafif Adnan Mohammed , Nasir Sobia , Bashir Jawad 
Published: July 2023

Purpose
Using trait activation theory (TAT), this paper aims to empirically assess that work drive (WD) personality characteristics of employees will express better towards intrapreneurial behaviour (IB) outcomes in higher levels of job autonomy (JA) at the workplace.

Design/methodology/approach
Using cross-sectional design, a total of 258 engineering employees from the automotive industry of Pakistan were surveyed. The partial least square structural equation modelling (PLS-SEM) statistical method was used to evaluate the hypothesised theoretical model.

Findings
Statistical results revealed a significant and positive relationship between WD and IB. WD personality was also found to be activated with its interplay with JA for personality expression towards IB.

Practical implications
The present study offers a deeper insight into the interplay of organisational and individual factors as determinants of IB of employees. The study's findings suggested the importance of the rightful application of organisational situational cues in person–situation interaction for positive workplace behaviours by employees. Personality assessment of employees and their interplay with situational cues, as per conceptualisation of TAT, can assist the corporations towards enhanced levels of employees' behavioural tendencies towards intrapreneurship.

Originality/value
The application of TAT from the present study's perspective is novel theoretically as the literature on the activation of personality traits towards IB outcomes is non-existent. Also, the assessment of situational cues in JA will open avenues for organisational behaviour researchers to seek more organisational situational moderators for their assessment of various personality–outcome relationships in diverse contexts to activate personality traits.
Market participation, market impact and marketing efficiency: an integrated market research on smallholder paddy farmers from Eastern India
Authors: Dey Shiladitya , Singh Piyush Kumar 
Published: July 2023

PurposeThe study aims to analyze the impact of market participation on small paddy farmers' income and consumption expenditure. The study also estimates various determinants affecting the market participation of smallholders. Further, the study computes the efficiency of different paddy marketing channels and identifies the determinants that impact the marketing channel selection of paddy growers in Eastern India.Design/methodology/approachThe study used the propensity score matching (PSM) approach to measure the impact of market participation on farm income and per capita consumption. Further, the study employed Acharya and Aggarwal's composite index approach to estimate the marketing efficiency of various paddy marketing channels. Further, a multinomial logit model was used to determine the marketing channel selection constraints.FindingsThe outcomes indicate that market participation positively impacts farm income and consumption expenditure. Education, membership in farmers' organizations, price information and distance to the marketplace significantly affect farmers' market participation. The results show that the producer–retailer marketing channel is the most efficient compared to others. However, most paddy farmers sell paddy to farmgate collectors due to a lack of market information, vehicle ownership, storage system, and inability to take the risk of venturing out of the farmgate into markets.Research limitations/implicationsThe study uses primary data and captures only farmers' perspectives to measure the impact of market participation, marketing channel efficiency and determinants for market channel selection. The other stakeholder's perceptions can be included in future studies.Originality/valueRarely does any study identifies the efficiency of different marketing channels for paddy farmers in India and includes cognitive factors like risk perception and trust in buyers as constraints for market channel selection.