bluuebunny/crossref_metadata_2025_split huggingface dataset:
Temporal and Spatial Expression of Parasitism-Related Olfactory Genes in Larvae of the Ectoparasitoid Dastarcus helophoroides (Coleoptera: Bothrideridae)
Authors: Yin Huayang , Li Xiaojuan , Dong Guangping , Guo Wanlin , Fang Jianmin , Liu Hongjian 
Published: June 2023

Abstract
Dastarcus helophoroides (Fairmaire) (Coleoptera: Bothrideridae) is an ectoparasitoid of the pine sawyer beetle, Monochamus alternatus Hope (Coleoptera: Cerambycidae). A sensitive and precise olfactory system is required for the accurate location of the coleopteran host by D. helophoroides neonates. Herein, we characterized the relative expression patterns of six representative olfactory-related genes at different stages of M. alternatus parasitism and in different body regions of D. helophoroides neonates. The genes encoding chemosensory protein 2 (DhelCSP2), odorant receptor 2 (DhelOR2), and ionotropic receptor 2 (DhelIR2) were significantly upregulated before parasitization was initiated, whereas the genes encoding odorant binding protein 8 (DhelOBP8), gustatory receptor 5 (DhelGR5), and sensory neuron membrane protein 1 (DhelSNMP1) were significantly upregulated 4–5 d after initiation of parasitism. In D. helophoroides neonates, four genes (DhelOBP8, DhelCSP2, DhelOR2, and DhelIR2) were significantly upregulated in the head compared with the thoracoabdominal region, and one gene (DhelGR5) was significantly upregulated in the thoracoabdominal area compared with the head. Double-stranded RNAs (dsRNAs) targeting the six olfactory-related genes were synthesized and delivered to D. helophoroides neonates via immersion. After dsRNA treatment, the transcript levels of four olfactory-related genes (DhelOBP8, DhelCSP2, DhelOR2, and DhelSNMP1) were significantly reduced compared with that of the controls. These results provide a basis for further functional explorations of D. helophoroides olfactory genes, which may lead to the development of improved biological pest control methods using D. helophoroides larvae.
An operational model for capturing grape ripening dynamics to support harvest decisions
Authors: Van Leeuwen Cornelis , Destrac-Irvine Agnès , Gowdy Mark , Farris Laura , Pieri Philippe , Marolleau Laura , Gambetta Gregory A. 
Published: June 2023

Grape ripening is a critical phenological phase during which many metabolites that impact wine quality accumulate in the berries. Major changes in berry composition include a rapid increase in sugar and a decrease in malic acid content and concentration. Its duration is highly variable depending on grapevine variety, climatic parameters, soil type and management practices. Together with the timing of mid-veraison, this duration determines when grapes can be harvested.Viticulturists and winemakers monitor the sugar-to-total acidity ratio (S/TA) during grape ripening and start harvesting grapes when this ratio reaches the optimum value for the desired wine style. The S/TA ratio evolves linearly as a function of thermal summation during the first four weeks following the onset of ripening. The linearity of the evolution of the S/TA ratio as a function of thermal time during the first four weeks following mid-veraison is applied in this study on two large data sets encompassing (1) 53 varieties studied during 10 years with two to four replicates for each combination of year and cultivar and (2) two varieties, cultivated on three soil types over 13 years. Grape ripening speed is highly variable. The effects of the year impact ripening speed more than the effects of the soil or the variety, although all three effects are highly significant. Grape ripening speed decreases with berry weight and also varies with vine water status. By using this approach, viticulturists and winemakers can assess four weeks after mid-veraison, for each individual vineyard parcel, at what speed grape ripening progresses. Combined with precise mid-veraison scoring, expertise from previous vintages and complementary approaches like sensory assessment of berries, it allows harvest date estimates to be fine-tuned. The results of this study can also be used to identify slow ripening varies, which are better performing in warm climates and, thus, better adapted to climate change.
Authors: Bela Gesit Marti , Suhardi Imam , Yanti Sri Nani Hari 
Published: June 2023

Penelitian ini membahas cerpen Siapa Namamu, Sandra? karya Norman Erikson Pasaribu yang mengisahkan tentang perjalanan seorang ibu yang mengalami kedukaan sesudah kematian anak tunggalnya. Cerpen tersebut memuat psikologis tokoh utama dan suasana atmosferik, tempat, atau objek. Berdasarkan hal tersebut, penelitian ini difokuskan untuk mengidentifikasi leksia-leksia yang dikategorikan sebagai kode semik dalam kajian semiotika Roland Barthes. Metode yang digunakan dalam penelitian ini adalah metode deskriptif kualitatif. Teknik pengumpulan data yang digunakan adalah teknik baca dan catat. Teknik analisis data yang digunakan adalah teknik analisis isi atau konten. Hasil penelitian menemukan 14 leksia kode semik. Adapun pemaknaan leksia-leksia tersebut menghasilkan 3 makna yang diperoleh dengan bantuan teori Psikoanalisis Jacques Lacan. Tiga makna tersebut yaitu kebahagiaan sebagai permintaan (demand), nama sebagai identitas, dan kehilangan. Makna-makna yang diperoleh menggambarkan fase ketidakutuhan dalam diri tokoh utama. Oleh karena itu, cerpen Siapa Namamu, Sandra? dapat dijadikan sebagai pembelajaran.
Kata kunci: semiotika, kode semik, leksia, makna
Estetika Cerpen Ahmad Tohari Dari Sudut Pandang Publik Seni
Authors: Setyaningrum Sri Nurhayati , Rahayu Selvia Era , Putri Evani Asiya Kusuma , Puspitasari Rini 
Published: June 2023

Cerita pendek merupakan karya sastra berupa prosa yang umumnya bercerita tentang hal nyata maupun fiksi. Tujuan penelitian ini ialah menganalisis unsur estetika dalam publik seni. Beberapa unsur yang dianalisis di antaranya interpretasi, apresiasi, isi, bentuk, gaya bahasa, dan evaluasi pada cerpen. Metode yang digunakan dalam penelitian ini ialah deskriptif kualitatif. Metode deskriptif diartikan sebagai prosedur pemecahan masalah yang diselidiki dengan menggambarkan atau melukiskan keadaan subjek atau objek penelitian (novel, drama, cerita pendek, dan puisi) berdasarkan fakta yang tampak dan sebagaimana adanya. Sedangkan metode kualitatif dimaksudkan penyampaian makna dari isi karya tersebut. Hasil penelitian dapat memunculkan analisis seni yang dilakukan oleh pembaca/publik seni sehingga dapat memunculkan nilai-nilai sosial yang dapat diwujudkan dalam bentuk empati maupun simpati atau hal positif lainnya.
Pengaruh Pemberian Vitamin E Terhadap Derajat Stress Oksidatif Akibat Olahraga
Authors: Ngadiman Ngadiman , Noor Heza Fuad , Suko Wahono Bayu 
Published: June 2023

Olahraga mempunyai dampak positif dan negatif. Dampak positif olahraga diantaranya adalah meningkatkan kebugaran dan kesehatan, meningkatkan prestasi dan mencegah berbagai penyakit. Salah satu dampak negatif olahraga adalah terbentuknya senyawa oksidan yang memicu peristiwa stres oksidatif. Stres oksidatif terjadi karena sistem pertahanan antioksidan tubuh tidak mampu mengimbangi produksi radikal bebas. Stress Oxidative (SOD) dan Malondialdehyde (MDA) merupakan penanda aktivitas biologis dalam organisme yang terpapar stres oksidatif dari radikal bebas. Vitamin E adalah contoh antioksidan eksogen yang memiliki kandungan berbagai komponen antioksidan. Penelitian ini bertujuan untuk mengetahui pengaruh pemberian vitamin E sebelum aktivitas fisik terhadap konsentrasi malondialdehida plasma dan derajat kerusakan SOD akibat stres oksidatif pada manusia setelah aktivitas fisik. Rancangan penelitian menggunakan desain kelompok kontrol uji coba pasca laboratorium dengan tiga kelompok yang masing-masing terdiri dari sembilan subjek. Pengukuran dilakukan dengan mengambil 3 cm3 darah sebelum dan sesudah aktivitas fisik submaksimal. Dari uji post hoc diketahui terdapat perbedaan yang bermakna (0,001) antara kelompok kontrol dan kelompok K1 (dosis vitamin E 250 mg). Terdapat perbedaan bermakna (0,000) antara kelompok kontrol dan kelompok K2 (vitamin E dosis 500mg). Terdapat perbedaan bermakna (0,006) antara kelompok perlakuan K1 (vitamin E dosis 250mg) dengan K2 (vitamin E dosis 500mg). Simpulan dari penelitian ini adalah pemberian antioksidan eksogen (vitamin E dosis 250 mg dan dosis 500 mg) sebelum aktivitas fisik selama tujuh hari dapat menurunkan kadar SOD dan MDA plasma
Seasonal changes in the ratio of congeneric charrs in northern Japanese tributaries with different temperature regimes
Authors: Yamada Taihei , Koizumi Itsuro , Nakamura Futoshi 
Published: June 2023

Abstract
Background: The species distributions and community compositions of stream fishes are often shaped by water temperature. Stream salmonids, for example, show longitudinal gradients or marked community differences between spring-fed vs. non-spring-fed streams, which may be a result of species-specific temperature preferences, interspecific competition, or their interaction (i.e., temperature-dependent competition). Despite the widely reported patterns, how such temperature-associated distributions change across seasons remains largely unknown. If temperature is the main driver, the pattern will change across seasons. Alternatively, seasonal patterns will be obscured if other factors, such as species-specific life histories or seasonal aggregation, are important. Here, we investigated the effects of water temperature on the abundance and species ratio of two potentially competing salmonids, southern Asian Dolly Varden Salvelinus curilus and white-spotted charr Salvelinus leucomaenis, in 6 small tributaries with different temperature regimes (i.e., spring-fed and non-spring-fed) across seasons.
Results: Across seasons, the southern Asian Dolly Varden dominated in spring-fed tributaries, whereas white-spotted charr dominated in non-spring-fed tributaries. The seasonal variation in the abundance of southern Asian Dolly Varden and species ratio were explained by water temperature fluctuation, whereas that of white-spotted charr was not explained by the temperature fluctuation. No winter aggregation was observed in any of the tributaries. In addition, only the species ratio in summer was explained by the water temperature, and the water temperature did not fully explain the species ratio.
Conclusions: Our results indicated that temperature preference and/or the strength of interspecific competition may differ across seasons, which is expected to determine the seasonal distribution patterns in spring-fed and non-spring-fed tributaries. This study underscores the importance of considering seasons when evaluating temperature effects on the distribution of stream salmonids.
SuRxgWell: Study Protocol for a Randomized Controlled Trial of Telemedicine-based digital Cognitive Behavioral Intervention for high Anxiety and Depression Among Patients Undergoing Elective Hip and Knee Replacement surgery
Authors: Kaynar Ata Murat , Lin Charles , Sanchez Andrea Gomez , Lavage Daniel R. , Monroe Amy , Zharichenko Nicole , Strassburger Meredith , Saucier Katheryn , Groff Yram J. , Klatt Brian A , O'Malley Michael J. , Szigethy Eva M. , Wasan Ajay D. , Chelly Jacques E 
Published: June 2023

Abstract
Background
Mood disorders (anxiety, depression) and catastrophizing, lead to increased post-operative pain perception, decreased engagement with physical activity, and increased resource utilization. Studies on perioperative psychological assessment and treatment are scarce. Digital cognitive behavioral intervention (dCBI) such as RxWelläreduces depression in patients with inflammatory bowel disease. We hypothesize that SuRxgWell, the surgical adaptation of RxWellä, can improve preoperative mood disorders and subsequently reduce postoperative pain and opioid requirement in patients undergoing primary total hip and knee arthroplasty (THA, TKA).
Methods
This randomized, controlled trial will enroll primary and unilateral THA or TKA patients with anxiety and/or depression symptoms before surgery to receive the SuRxgWell. After an informed consent, subjects will be screened using the PROMIS Anxiety/Depression questionnaires. Subjects with a T-score of ≥ 60 will be randomized to either usual care (control group) or SuRxgWell dCBI pre- and perioperative cognitive behavioral intervention via RxWell plus usual care (intervention group). Control group will receive information on how to locate tools to address mood disorders, whereas the intervention group will have access to SuRxgWell 1 month prior to surgery and up to 3 months thereafter. The allocation will be 3:1 (intervention: control). Investigators will be blinded, but research coordinators and subjects will not.
The primary outcome will be day of surgery anxiety or depression symptoms measured with the PROMIS Anxiety/Depression, GAD-7, and PHQ-8. Secondary endpoints include sleep disturbance, fatigue, ability to participate in social roles, pain interference, cognitive function, pain catastrophizing, and physical function as well as preoperative and postoperative pain scores, pain medication usage, orthopedic functional recovery at baseline, day of surgery, and 1, 2, and 3 months after the surgery. Subjects will complete a GAD-7 and PHQ-8 questionnaires bi-weekly. Data about RxWell usage and engagement, postsurgical complications, and resource utilization will be recorded. The primary outcome will be analyzed with intention-to-treat estimates and tested using independent two sample t-tests.
Discussion
We will evaluate dCBI as a perioperative tool to improve anxiety and depression before and after major orthopedic surgery. If effective, this might have important clinical implications towards improving outcomes.
Trial Registration
clinicaltrials.gov, NCT05658796. Registered 21 December 2022. https://clinicaltrials.gov/ct2/show/NCT05658796
Blood-conserving and therapeutic efficacy of intravenous tranexamic acid at different time points in primary total knee arthroplasty with tourniquet application: a randomized controlled trial
Authors: Wang Mingyou , Lan Yuping , Wang Hongping , Chen Chunyu , Mei Zhu , Tao Qifeng 
Published: June 2023

Abstract
Objective
To investigate the effect of the first dose of tranexamic acid received intravenously before tourniquet compression and release in total knee arthroplasty (TKA) on perioperative blood loss and therapeutic efficacy of patients.
Methods
In this double-blind trial, 90 patients undergoing primary TKA were randomized into 2 groups: the patients in group A was received intravenous tranexamic acid (TXA) 10 minutes before tourniquet compression (20 mg/kg) and 3, 6 and 24 hours later (10 mg/kg), and the patients in Group B was treated the same as Group A but received intravenous TXA before tourniquet release. The primary outcomes were the change of blood loss, hemoglobin and hematocrit. Secondary outcomes included operation and tourniquet time, blood transfusion rate, subcutaneous petechiae and circumferential changes of the operated limb, visual analog scale (VAS), hospital for special surgery (HSS) score, length of stay (LOS) postoperatively, complications and patient satisfaction.
Results
No statistically significant differences were found between the 2 groups with regard to age, gender, weight, BMI, Kellgren-Lawrence class, preoperative blood volume, preoperative laboratory values, operation and tourniquet time, transfusion rate, knee circumference, preoperative HSS, VAS. There was no significant difference in intraoperative blood loss (IBL) (52.71 ml vs. 63.44 ml, P= 0.07), hidden blood loss (HBL) (91.39 ml vs. 119.90, P= 0.40) and total blood loss (TBL) (144.1 ml vs. 183.3 ml, P= 0.72) between groups A and B. Hemoglobin, hematocrit and red blood cell count (RBC) dropped to a low point on postoperative day 3 and then rebounded, returning to normal levels on day 21, and the trend of change between the 2 groups was no statistically significant difference. There was no significant difference in subcutaneous ecchymosis, knee swelling, HSS, VAS, LOS postoperatively, complication rate and patient satisfaction.
Conclusion
There was no significant difference in total perioperative blood loss, knee swelling, subcutaneous bruising and petechiae, knee function, complication rate and satisfaction between the intravenous tranexamic acid before tourniquet compression and release in primary total knee arthroplasty. However, the trend towards better reduction of perioperative blood loss, as reflected by changes in blood loss and h ematocrit, may be related to the role of the effective blood concentration formed in the operative area before tourniquet pressurization. Although it is not possible to conclude that better hemostasis can be achieved by administration before tourniquet compression, we believes that 10 minutes before tourniquet compression is a more recommendable option when conditions permit, and that the second postoperative dose should be dripped intravenously 3 hours after the first dose to ensure effective plasma concentrations.
Monitoring of electricity generation from exhaust waste heat and wireless data recording from a mobile phone in real driving conditions of a vehicle
Authors: Akal Dinçer , umut ilhan 
Published: June 2023

Abstract
This study is aimed to design electrical energy production and wireless data monitoring system by utilizing the working principles of thermoelectric generators from the exhaust waste heat of vehicles in traffic. In order to transmit the heat transfer in the exhaust pipe to the thermoelectric generators, a modular system has been developed in a hexagonal form that can be dismounted and mounted from aluminum material. Six thermoelectric generators (TEG-SP1848) are used on the aluminum module, and a heat sink is used to cool each thermoelectric generator. The voltage and current values of the electrical energy produced from the exhaust waste heat of the vehicle in real driving conditions are recorded with a special program written on the card on the system. Depending on the results obtained from the test drives, electrical energy was obtained at a maximum voltage of 9.8 V and a current of 0.32 A. This electrical energy obtained from the exhaust waste heat can be stored in the vehicle's existing battery if desired. In this way, since the alternator used for the electricity generation of the vehicle will be activated less, some fuel savings will be achieved in the engine, and harmful exhaust emissions will be reduced. In addition, the electrical energy obtained by this method can be stored in an external battery independent of the vehicle battery and used for various purposes when the vehicle is at a standstill or with a portable battery.
Expression of long non coding RNA H19&amp; miR-675 in colorectal cancer and ulcerative colitis patients
Authors: Sobhy Rania , Shaker Olfat , Khairy Ahmed , Gaber Dalia A. 
Published: June 2023

Abstract

Background: Colorectal cancer (CRC) is a growing public health problem and has been stretching in the recent years from old age group to encompass younger age group. Inflammatory bowel disease like ulcerative colitis is a risk factor for CRC.
Aim: Test the expression of microRNA- 675 (miR-675) and long non coding RNA H19 (Lnc H19) for their diagnostic & prognostic potential in CRC.
Methodology: A prospective cross-sectional study was conducted on 60 patients with CRC, 60 patients with UC as well as 30 control subjects. miR-675 & lnc H19 were quantified by real time RT-PCR. Lab investigations and radiological investigations were also performed and correlated with the genetic biomarkers.
Results: Lnc H19 was highly expressed in both CRC and UC groups with a statistically significant difference from the control group. There was a significant enhanced expression of H19 in CRC compared to UC group. Although miRNA -675 was down regulated in both UC and CRC groups but it was more expressed in CRC compared to UC group.
Conclusion: According to our results we can conclude that H19 can be considered as potential non-invasive biomarker for diagnosis of UC and early detection of CRC
ERASURE: Early autologous blood pleurodesis for postoperative air leaks - A randomized, controlled trial comparing prophylactic autologous blood pleurodesis versus standard watch and wait treatment for postoperative air leaks following thoracoscopic (VATS) anatomic lung resections.
Authors: Karampinis Ioannis , Ruckes Christian , Doerr Fabian , Boeluekbas Servet , Ricciardi Sara , Cardillo Giuseppe , Galvez Carlos , Vidmar Bogdan , Stupnik Tomaz , Fang Vincent , Petersen Rene Horsleben , Roessner Eric Dominic 
Published: June 2023

Abstract
• Background: The prolonged air leak is probably the most common complication following lung resections. Around 10-20% of the patients that undergo a lung resection will eventually develop a prolonged air leak. The definition of a prolonged air leak varies between an air leak, which is evident after the fifth, seven or even tenth postoperative day to every air leak that prolongs the hospital stay. However, the postoperative hospital stay following a thoracoscopic lobectomy can be as short as two days, making the above definitions sound outdated. The treatment of these air leaks is also very versatile. One of the broadly accepted treatment options is the autologous blood pleurodesis or “blood patch”. The purpose of this trial is to investigate the impact of a prophylactic autologous blood pleurodesis on reducing the duration of the postoperative air leak and therefore prevent the air leak from becoming prolonged.
• Methods: Patients undergoing an elective thoracoscopic anatomic lung resection for primary lung cancer or metastatic disease will be eligible for recruitment. Patients with an air leak of > 100ml/min within 6 hours prior to the morning round on the second postoperative day will be eligible for inclusion in the study and randomization. Patients will be randomized to either blood pleurodesis or watchful waiting. Primary endpoint is the time to drain removal measured in full days. The trial ends on the seventh postoperative day.
• Discussion: The early autologous blood pleurodesis could lead to a faster cessation of the air leak and therefore to a faster removal of the drain. A faster removal of the drain would relieve the patient from all the well-known drain associated complications (longer hospital stay, stronger postoperative pain, risk of drain-associated infection etc.). From the economical point of view, faster drain removal would reduce the hospital costs as well as the costs associated with the care of a patient with a chest drain on an outpatient setting.
Trial registration: The study has been prospectively registered in the German Clinical Trials Register (DRKS, DRKS-ID: DRKS00030810)
Vascular Wall Vibrations in Arteriovenous Fistula; A Specific Stimulus for Stenosis Development and AVF Failure?
Authors: Bozzetto michela , Remuzzi Andrea , Valen-sendstad kristian 
Published: June 2023

Abstract
Hemodialysis is the lifeline for nearly three million end stage renal disease patients worldwide. Native arteriovenous fistula (AVF) is the preferred vascular access, but 40% fail within 1 year. We recently demonstrated that AVFs harbour transitional flows and the goal of the present study was to investigate whether the associated high-frequency pressure fluctuations could promote vibrations within the vascular wall. We acquired MRI images and flow rates immediately after surgery in one patient and generated a 3D patient-specific model. High-fidelity fluid structure interaction simulations revealed the presence of wall vibrations in distinct frequency bands up to 200 Hz and amplitude of 200 microns. A sensitivity analysis to assess the impact of flow rates, and vascular wall stiffness and thickness, changes that typically occur during AVF maturation, confirmed the robustness of the results. Interestingly, the vibrations were always predominant at the anastomosis floor and on the inner venous side, which correlates with typical stenotic regions. As studies seeking to correlate aberrant stresses and vascular remodelling have been largely inconclusive, the focal collocation between vibrations and stenosis may suggest an unknown mechanobiological process between high-frequency mechanical stresses within the vascular wall and adverse vascular remodelling.