bluuebunny/crossref_metadata_2025_split huggingface dataset:
Dead‐end hollow fiber ultrafiltration capture of environmental DNA for freshwater mussel (Unionidae) species detection with metabarcoding
Authors: McKee Anna M. , Klymus Katy E. , Lor Yer , Kaminski Marissa , Tajjioui Tariq , Johnson Nathan A. , Carroll Matt , Goodson Chris , Spear Stephen F. 
Published: September 2023

AbstractInsufficient water sample volumes can be a limiting factor for detecting species with environmental DNA (eDNA) from aquatic habitats. We compared detections of freshwater mussel (Unionidae) communities using large water sample volumes and dead‐end hollow fiber ultrafiltration (D‐HFUF or DEUF) with traditional eDNA filtration methods that use relatively small water sample volumes. Unionid species were detected in approximately 50‐L D‐HFUF eDNA samples with two mitochondrial DNA metabarcoding markers (COI and ND1) and compared to species detection results from eDNA captured from commonly used 1‐L samples filtered with polyethersulfone (PES) filters at three lotic sites in Georgia and Missouri. Of the 431,560 COI and 1,035,472 ND1 reads from all environmental samples of both filter types that passed quality control, 95% (410,755 reads) of COI reads and 85% (883,472 reads) of ND1 reads were assigned to a unionid species. Nineteen different freshwater mussel species were detected across all D‐HFUF samples, and 11 species were detected across all PES samples. Reads assigned to the genus Elliptio could not be resolved beyond the genus level with either marker. From D‐HFUF samples, 15 and 16 mussel species were detected with the COI and ND1 markers, respectively. From PES samples, nine and seven species were detected with the COI and ND1 markers, respectively. More mussel species were detected at each site in D‐HFUF samples than in PES samples regardless of whether results from both markers were combined or evaluated separately. Our results demonstrate the merit of further exploration and optimization of D‐HFUF for capturing eDNA from high‐volume water samples to facilitate detection of unionids and likely other aquatic organisms.
Evaluation of vector magnetic property in a three‐phase induction motor model core with visualization of streamline
Authors: Kai Yuichiro , Maeda Ryoya , Enokizono Masato 
Published: September 2023

AbstractIn this paper, a streamline display method for magnetic flux density and magnetic field strength vectors in a three‐phase induction motor model core is presented, and the relationship between streamlines and magnetic properties is investigated. It is possible to visualize the vector magnetic properties distribution by representing the magnetic flux density and the magnetic field strength vector with the streamlines. In addition, the time variation of the streamlines of the magnetic flux density and magnetic field strength vectors, and magnetic energy was compared. It was clear that the magnetic energy did not necessarily increase in the areas where the magnetic flux density and magnetic field strength increased because these vectors have the spatial phase difference.
Enantioselective Construction of Axially Iodobenzocarbazole Derivatives by Stereogenic‐at‐Cobalt(III)‐Complex‐Catalyzed Iodoarylation of Alkynes
Authors: Yao Chuan‐Zhi , Zhao Zi‐Yuan , Tu Xue‐Qin , Xie Zu‐Kui , Jiang Hua‐Jie , Li Qiankun , Yu Jie 
Published: September 2023

AbstractA new synthetic approach to novel axially chiral iodobenzocarbazole derivatives based on the highly enantioselective intramolecular iodoarylation of linked alkyne‐indole systems was developed by using the versatile chiral catalyst, stereogenic‐at‐cobalt(III)‐complex, through an axially chiral iodinated vinylidene o‐quinone methide (IVQM) intermediate. This protocol provides 21 examples in excellent yields with good to high enantioselectivities (up to 96 % yield, 98 % ee). Furthermore, the introduced iodine atoms can easily be converted into other functional groups.
Pain experience in eating disorders: The mediating role of depression, alexithymia and interoceptive awareness
Authors: Fazia Gilda , Carbone Elvira Anna , Rania Marianna , Quirino Daria , Aloi Matteo , de Filippis Renato , De Fazio Pasquale , Colloca Luana , Segura‐García Cristina 
Published: September 2023

AbstractObjectiveEvidence suggests reduced sensitivity to pain due to high pain threshold in anorexia and bulimia nervosa and a possible role of depression, alexithymia and interoceptive awareness on pain experience. This study examined whether self‐report and real‐time evoked pain experience were mediated by depression, alexithymia and interoceptive awareness in a comprehensive sample of patients with eating disorders (ED).Method145 participants (90 ED, 55 healthy controls (HC)) underwent a real‐time evoked examination of pain and completed self‐report questionnaires for pain (Pain Detect Questionnaire (PD‐Q), PD‐Q VAS, Leeds Assessment of Neuropathic Symptoms and Signs), depression (BDI‐II), interoceptive awareness Multidimensional Assessment of Interoceptive Awareness (MAIA), and alexithymia (TAS‐20). Three mediation models, with ED diagnosis as independent variable, and BDI, MAIA and TAS‐20 as mediators, were tested.ResultsParticipants with ED and HC exhibited similar pain type and intensity (self‐report and real‐time). Eating disorders diagnosis was associated with lower self‐report pain intensity and non‐neuropathic like pain experience (model 1–2). Depressive symptoms partially (model 1–2) or fully (model 3) mediated the association between ED diagnosis and pain experience, alone (model 1) or via alexithymia (model 3). Interoceptive awareness did not influence pain symptomatology.DiscussionED diagnosis is associated with non‐neuropathic and lower pain experience. However, concurrent depression and alexithymia are associated with higher pain symptoms and neuropathic features. These results could inform clinicians about the influence of psychopathology on pain experience in ED.
Potato peel waste fermentation by Rhizopus oryzae to produce lactic acid and ethanol
Authors: Ozer Uyar Gülsüm Ebru , Uyar Basar 
Published: September 2023

AbstractPotato peel waste (PPW), a zero‐value by‐product generated from potato processing, is a promising fermentation substrate due to its large quantity of starch, nonstarch polysaccharides, lignin, protein, and lipid. Rhizopus oryzae is a filamentous fungus that is mainly known as a lactic acid producer and can ferment various agro‐wastes. This study aimed to use R. oryzae for the fermentation of PPW. A series of batch fermentations were conducted to investigate the effects of different PPW loading rates (2%–8%) and particle sizes (0–4 mm). Under an initial PPW loading rate of 8% and particle size of 1–2 mm, the maximum ethanol (18.83 g/L) and lactic acid (3.14 g/L) concentrations, the highest ethanol (9.41 g/L·day) and lactic acid (1.89 g/L·day) average production rates were obtained. Under these conditions, the yield of ethanol and lactic acid was 0.235 g/gPPW and 0.039 g/gPPW, respectively. R. oryzae was shown to utilize PPW as a substrate to produce value‐added bioproducts such as ethanol (major product) and lactic acid.
Petrographic composition and heavy minerals in modern river sand: A global database
Authors: Liang Wendong , Hu Xiumian , Garzanti Eduardo , Wen Huaguo , Hou Mingcai 
Published: September 2023

AbstractIn the case of modern river sediments, source rocks, tectonic setting, and climatic and geomorphological conditions are fully known, diagenetic effects are by definition none, and physical and chemical processes during erosion, transport, and deposition can be investigated directly in any required detail. Their study thus provides the fundamental information needed to understand the impact of various controls on sediment generation and sediment routing systems in diverse geological settings. With the growing interest in source‐to‐sink sedimentary systems for economic purposes, researchers have focused more and more on modern environments, producing an ever‐increasing amount of compositional data on river sediments. To better integrate and utilize these large datasets, we systematically compiled a global database of 4,208 fluvial sediment samples compiled from 100 published papers, book chapters, and dissertations and included 3,747 petrographic and 1,943 heavy‐mineral analyses. The analysed samples are mostly located in Asia, Africa, Europe, and South America, with fewer examples from North America and Oceania. Each sample is complemented by metadata describing references, river name, geographic location, sampling date, analytical method, and grain size, so that samples can be grouped and filtered to meet different needs including comparison with other datasets.
Chronic psychosocial stress triggers microglial‐/macrophage‐induced inflammatory responses leading to neuronal dysfunction and depressive‐related behavior
Authors: Kokkosis Alexandros G. , Madeira Miguel M. , Hage Zachary , Valais Kimonas , Koliatsis Dimitris , Resutov Emran , Tsirka Stella E. 
Published: September 2023

AbstractChronic environmental stress and traumatic social experiences induce maladaptive behavioral changes and is a risk factor for major depressive disorder (MDD) and various anxiety‐related psychiatric disorders. Clinical studies and animal models of chronic stress have reported that symptom severity is correlated with innate immune responses and upregulation of neuroinflammatory cytokine signaling in brain areas implicated in mood regulation (mPFC; medial Prefrontal Cortex). Despite increasing evidence implicating impairments of neuroplasticity and synaptic signaling deficits into the pathophysiology of stress‐related mental disorders, how microglia may modulate neuronal homeostasis in response to chronic stress has not been defined. Here, using the repeated social defeat stress (RSDS) mouse model we demonstrate that microglial‐induced inflammatory responses are regulating neuronal plasticity associated with psychosocial stress. Specifically, we show that chronic stress induces a rapid activation and proliferation of microglia as well as macrophage infiltration in the mPFC, and these processes are spatially related to neuronal activation. Moreover, we report a significant association of microglial inflammatory responses with susceptibility or resilience to chronic stress. In addition, we find that exposure to chronic stress exacerbates phagocytosis of synaptic elements and deficits in neuronal plasticity. Importantly, by utilizing two different CSF1R inhibitors (the brain penetrant PLX5622 and the non‐penetrant PLX73086) we highlight a crucial role for microglia (and secondarily macrophages) in catalyzing the pathological manifestations linked to psychosocial stress in the mPFC and the resulting behavioral deficits usually associated with depression.
Route sequence knowledge supports the formation of cognitive maps
Authors: Hilton Christopher , Wiener Jan 
Published: September 2023

AbstractIn this study, we examined the extent to which knowledge about the sequence of places encountered during route learning supports the formation of a metric cognitive map. In a between subjects design, participants learned a route until they could navigate it independently without error whilst also learning information about either the identity of places along the route (Recognition Learning condition) or the sequence of places along the route (Sequence Learning condition). In a follow‐up Reconstruction of Order Task, we confirmed that participants in the Sequence Learning condition had more accurate route sequence knowledge than those in the Recognition Learning condition, despite requiring the same overall number of trials to learn the route. Participants then completed a Pointing Task to assess the quality of their cognitive map of the environment. Both groups performed above chance level, showing incidental encoding of metric information, but the Sequence Learning group produced significantly lower pointing errors than the Recognition Learning group. Further, we found that route distance between pairs of places was a strong predictor of pointing error in both groups, whilst Euclidean distance between places was a significant, but weak, predictor only for the Sequence Learning condition. The results of this study demonstrate that discrete route sequence knowledge directly supports the formation of metric cognitive maps. We consider how the results are best explained by interactions between striatal route representations and hippocampal metric representations, centered around the sequence of places acting as a scaffold for the encoding of metric information.
Total Synthesis of Salviachinensine A Using a Matteson Homologation Approach
Authors: Kempf Merlin , Andler Oliver , Kazmaier Uli 
Published: September 2023

AbstractSalviachinensine A, a natural product obtained from the Chinese medicinal plant Salvia chinensis, was synthesized for the first time by employing Donald Matteson's boronic ester homologation. The use of (R,R)‐dicyclohexylethane‐1,2‐diol (DICHED) enabled the generation of the required stereogenic centers in a highly stereoselective fashion. Salviachinensine A was formed in a one‐pot protecting group cleavage/lactonization/ intramolecular Friedel–Crafts alkylation, followed by Lewis acid‐catalysed cleavage of the acetal protecting groups.
Effects of adoptive T‐cell immunotherapy on immune cell profiles and prognosis of patients with unresectable or recurrent cholangiocarcinoma
Authors: Kida Akihiko , Mizukoshi Eishiro , Kitahara Masaaki , Miyashita Tomoharu , Goto Shigenori , Kamigaki Takashi , Takimoto Rishu , Asai Jun , Kakinoki Kaheita , Urabe Takeshi , Tomita Katsuro , Kaneko Shuichi 
Published: September 2023

AbstractThe identification of immune cell profiles (ICP) involved in anti‐tumor immunity is crucial for immunotherapy. Therefore, we herein investigated cholangiocarcinoma patients (CCA) who received adoptive T‐cell immunotherapy (ATI). Eighteen unresectable or recurrent CCA received ATI of αβ T cells alone or combined with chemotherapy. ICP were evaluated by flow cytometry. There were 14 patients with intrahepatic cholangiocarcinoma (iCCA) and four with distal cholangiocarcinoma (dCCA). After one course of treatment, nine iCCA and four dCCA had progressive disease (PD), while five iCCA had stable disease (SD). Median overall survival (OS) was prolonged to 21.9 months. No significant differences were observed in OS between the PD and SD groups of iCCA. The frequency of helper T cells (HT) in iCCA decreased from 70.3% to 65.5% (P = .008), while that of killer T cells (KT) increased from 27.0% to 30.6% (P = .005). dCCA showed no significant changes of immune cells. OS was prolonged in iCCA with increased frequencies of CD3+ T cells (CD3) (P = .039) and αβ T cells (αβ) (P = .039). dCCA showed no immune cells associated with OS. The frequencies of CD3+ T cells and αβ T cells in the PD group for iCCA decreased from 63.5% to 53% (P = .038) and from 61.6% to 52.2% (P = .028), respectively. In the SD group, the frequency of HT decreased from 65.8% to 56.9% (P = .043), whereas that of KT increased from 30.1% to 38.3% (P = .043). In conclusions, ATI affected ICP and prolonged OS. Immune cells involved in treatment effects differed according to the site of cholangiocarcinoma.
Partner testing with HIV self‐test distribution by Ugandan pregnant women living with HIV: a randomized trial
Authors: Mujugira Andrew , Nakyanzi Agnes , Donnell Deborah , Boyer Jade , Stein Gabrielle , Bulterys Michelle , Naddunga Faith , Kyomugisha Juliet , Birungi Juliet E. , Ssendiwala Paul , Nsubuga Rogers , Muwonge Timothy R. , Musinguzi Joshua , Sharma Monisha , Celum Connie L. 
Published: September 2023

AbstractIntroductionSecondary distribution of HIV self‐tests (HIVST) by HIV‐negative pregnant women to male partners increases men's testing rates. We examined whether this strategy promotes male partner testing for pregnant women living with HIV (PWLHIV).MethodsWe conducted an open‐label individually randomized trial in Kampala, Uganda, in which PWLHIV ≥18 years who reported a partner of unknown HIV status were randomized 2:1 to secondary distribution of HIVST for male partner(s) or standard‐of‐care (SOC; invitation letter to male partner for fast‐track testing). Women were followed until 12 months post‐partum. Male partners were offered confirmatory HIV testing and facilitated linkage to antiretroviral treatment (ART) or oral pre‐exposure prophylaxis (PrEP). Using intention‐to‐treat analysis, primary outcomes were male partner testing at the clinic and initiation on PrEP or ART evaluated through 12 months post‐partum (ClinicalTrials.gov, NCT03484533).ResultsFrom November 2018 to March 2020, 500 PWLHIV were enrolled with a median age of 27 years (interquartile range [IQR] 23–30); 332 were randomized to HIVST and 168 to SOC with 437 PWLHIV (87.4%) completing 12 months follow‐up post‐partum. Of 236 male partners who tested at the clinic and enrolled (47.2%), their median age was 31 years (IQR 27–36), 45 (88.3%) men with HIV started ART and 113 (61.1%) HIV‐negative men started PrEP. There was no intervention effect on male partner testing (hazard ratio [HR] 1.04; 95% confidence interval [CI]: 0.79–1.37) or time to ART or PrEP initiation (HR 0.96; 95% CI: 0.69–1.33). Two male partners and two infants acquired HIV for an incidence of 0.99 per 100 person‐years (95% CI: 0.12–3.58) and 1.46 per 100 person‐years (95% CI: 0.18%–5.28%), respectively. Social harms related to study participation were experienced by six women (HIVST = 5, SOC = 1).ConclusionsAlmost half of the partners of Ugandan PWLHIV tested for HIV with similar HIV testing rates and linkage to ART or PrEP among the secondary distribution of HIVST and SOC arms. Although half of men became aware of their HIV serostatus and linked to services, additional strategies to reach male partners of women in antenatal care are needed to increase HIV testing and linkage to services among men.
Facile extraction of berberine from different plants, separation, and identification by thin‐layer chromatography, high‐performance liquid chromatography, and biological evaluation against Leishmaniosis
Authors: Alam Syed Dilshad , Beg Mirza Adil , Bagadi Muralidhararao , Locatelli Marcello , ALOthman Zeid A. , Mustaqeem Mohd , Ali Imran 
Published: September 2023

The extraction of berberine was carried out from Berberis vulgaris, Berberis aquifolium, and Hydrastis canadensis plants using ethanol and water (70:30, v/v). The extracted berberine was characterized by ultraviolet‐visible and Fourier‐transform infrared spectroscopy. The purity of berberine was ascertained by thin‐layer chromatography using n‐propanol‐formic acid‐water (95:1:4) and (90:1:9) solvents. hRf values were in the range of 44–49 with compact spots (diameter 0.2–0.4 cm). HPLC was carried out using ammonium acetate buffer and acetonitrile in gradient mode with Zodiac (4.6 × 150 mm, 3 μm) column. The flow rate was 1.0 mL/min and detection was at 220 nm. The values of separation and resolution factors of the standard and the extracted berberine were in the range of 1.13–1.16 and 1.40–1.71, respectively. A comparison has shown that both thin‐layer chromatography and high‐performance liquid chromatography (HPLC) methods found applications in different situations and requirements. The extracted berberine samples were used to treat Leishmaniosis and the results showed better activity of berberine in comparison to the standard drug Amphotericin B. Briefly, the reported research is a novel and may be used to extract berberine from plants, separation and identification of berberine by thin layer chromatography and HPLC and to treat Leishmaniosis.
Impacts of infiltration holes on rainfall‐runoff production on slopes treated with engineering measures: A simulation study in the Loess Plateau of China
Authors: Li Gaoliang , Wang Ke , Zheng Jiyong , Zheng Yi , Ma Zhanghuai , Zhang Xiaoyuan , Yang Kaiqi 
Published: September 2023

AbstractPresent slope engineering measures are insufficient to intercept and infiltrate extreme rainstorm runoff in the Loess Plateau of China. The infiltration hole that has specific depth and volume can be used as a supplemental to slope engineering measures to rapidly reduce rainstorm runoff and store them into deep soil layers. In this study, the performance of infiltration holes (60, 80, and 100 cm in depth and 10 cm in diameter) in intercepting and infiltrating rainstorm runoff was evaluated through the field simulation experiment (rainfall intensity of 1.5 mm min−1). The results showed that infiltration holes directly increased the runoff infiltration area by 0.20, 0.26, and 0.32 m2. Before infiltration holes were filled up, all the runoff were intercepted, the reduced runoff were 5.70, 5.85, 8.85 mm, respectively. When runoff reducing rate reached stable (around 110 min), the stable runoff‐reducing rates were 0.079, 0.088, and 0.091 mm min−1, and the cumulative reduced runoff were 15.05, 18.11, and 20.97 mm, accounting for 30.41%, 36.59%, and 42.36% of the simulated rainstorm runoff. This implies that filtration holes could, on average, reduce runoff by 70.50, 132.42, and 256.26 mm within 12, 24, and 48 h, respectively. Based on the results, it was verified that infiltration holes could reduce most of the runoff under the scenario of historical extreme rainstorms in the past two decades. Our research determined the rainfall runoff reduced by infiltration holes and verified the effectiveness of infiltration holes under rainstorm events, and provided a prospective technique for regulating extreme rainstorm runoff and decreasing landslides and mudslides risks on the Loess Plateau.
Correlation between the efficacy of picosecond‐domain laser treatment for solar lentigo and the vascularity in the upper dermis using optical coherence tomography angiography in Asian women
Authors: Hara Yusuke , Ninomiya Masato , Yamashita Toyonobu , Negishi Kei 
Published: September 2023

AbstractObjectivesThe purpose of this study was to noninvasively confirm the characteristics of the dermal vasculature in patients with solar lentigo (SL) and determine any association with the efficacy of picosecond‐domain laser (PSL) treatment.MethodsThirteen facial SL lesions in 11 Asian female patients were included in this study and evaluated over 12 weeks. An Nd:YAG laser was used at 532 nm and 750 ps. Skin color and morphological structure were evaluated by ANTERA‐3D® and optical coherence tomography (OCT), respectively. To analyze the vascularity in the upper dermis, an OCT angiography (OCTA) algorithm was applied to the OCT data.ResultsAfter PSL treatment, significant improvement in both hyperpigmentation and abnormally thickened epidermis was observed, but the efficacy varied for each lesion. There was a significant correlation between the change in the melanin index due to PSL treatment and preoperative vascular density in the upper dermis.ConclusionsTo the best of our knowledge, this is the first report to demonstrate a correlation between the efficacy of PSL treatment of SL lesions and the vascularity in the upper dermis. Methods to evaluate the vasculature in the upper dermis may be useful for preoperative prediction of the efficacy of PSL treatment for SL lesions.