bluuebunny/crossref_metadata_2025_split huggingface dataset:
The Cyanobacterial “Nutraceutical” Phycocyanobilin Inhibits Cysteine Protease Legumain
Authors: Wilkinson Isabel V. L. , Castro‐Falcón Gabriel , Roda‐Serrat Maria C. , Purdy Trevor N. , Straetener Jan , Brauny Melanie M. , Maier Lisa , Brötz‐Oesterhelt Heike , Christensen Lars P. , Sieber Stephan A. , Hughes Chambers C. 
Published: December 2022

AbstractThe blue biliprotein phycocyanin, produced by photo‐autotrophic cyanobacteria including spirulina (Arthrospira) and marketed as a natural food supplement or “nutraceutical,” is reported to have anti‐inflammatory, antioxidant, immunomodulatory, and anticancer activity. These diverse biological activities have been specifically attributed to the phycocyanin chromophore, phycocyanobilin (PCB). However, the mechanism of action of PCB and the molecular targets responsible for the beneficial properties of PCB are not well understood. We have developed a procedure to rapidly cleave the PCB pigment from phycocyanin by ethanolysis and then characterized it as an electrophilic natural product that interacts covalently with thiol nucleophiles but lacks any appreciable cytotoxicity or antibacterial activity against common pathogens and gut microbes. We then designed alkyne‐bearing PCB probes for use in chemical proteomics target deconvolution studies. Target identification and validation revealed the cysteine protease legumain (also known as asparaginyl endopeptidase, AEP) to be a target of PCB. Inhibition of this target may account for PCB's diverse reported biological activities.
Identification of quantitative trait loci for γ‐oryzanol concentration using a cross between cultivars with extremely high and low γ‐oryzanol levels
Authors: Nakano Hiroshi , Takai Toshiyuki , Kondo Motohiko 
Published: December 2022

AbstractBackground and ObjectiveAside from its various biomedical effects, γ‐oryzanol is a biomarker for heat stress in rice (Oryza sativa L.). Recently, we found that a japonica cultivar Momiroman had the highest γ‐oryzanol among 92 cultivars. In the present study, we aimed to detect the quantitative trait locus (QTLs) for the concentrations of cycloartenyl ferulate (1), 24‐methylenecycloartanyl ferulate (2), and total γ‐oryzanol in the populations from a cross between Momiroman and an indica cultivar Takanari.FindingsWe detected the QTLs for the concentrations of 1, 2, and/or total γ‐oryzanol on chromosomes 4, 5, 9, and 11. The Momiroman alleles at the QTLs on chromosomes 4, 5, and 11 increased the concentrations of 1, 2, and/or total γ‐oryzanol, whereas the Takanari alleles at the QTLs on chromosome 9 increased.ConclusionConsidering the literature data, the japonica alleles at the QTLs on chromosomes 4, 5, and 11 may increase the concentrations of 1, 2, and/or total γ‐oryzanol in the indica genetic background.Significance and NoveltyThis study revealed the QTLs for the concentrations of 1, 2, and/or total γ‐oryzanol on chromosomes 4, 5, 9, and 11, providing essential information for the development of cultivars with specific biomedical properties or heat stress resistance.
Ciprofloxacin induced toxic epidermal necrolysis with cholestatic hepatitis: A case report with literature review and revisit to the Naranjo adverse drug reaction probability scale
Authors: Dhakal Bishal , Dawadi Sagun , Khadka Laxman , Bohara Sujan , Neupane Shashank , Ale Magar Priajan , Pathak Bishnu Deep , Upadhaya Regmi Binit 
Published: December 2022

AbstractCiprofloxacin, among the many Fluoroquinolones, has been widely used as a broad‐spectrum antibiotic due to its wide range of action and relatively safe adverse effect profile. However, among the cutaneous adverse drug reactions due to Ciprofloxacin, toxic epidermal necrolysis occurring along with cholestatic hepatitis is a rare one. Here, we present a case of a 22‐year‐old male patient who was diagnosed with toxic epidermal necrolysis with cholestatic hepatitis secondary to Ciprofloxacin. Naranjo adverse drug reaction probability scale was used for the causal association.
A case of pericarditis in a middle‐aged woman with COVID‐19
Authors: Kushima Hisako , Shimizu Sayaka , Koide Yohei , Kawamura Akira , Ishii Hiroshi 
Published: December 2022

AbstractThe frequency of pericarditis as a complication in COVID‐19 patients without underlying disease is not well known. We report a case of COVID‐19 presenting with pericarditis without myocarditis or severe respiratory symptoms in a middle‐aged woman, who had neither underlying disease nor previous diagnosis of COVID‐19.
Childhood Guillain–Barre syndrome in the SARS‐CoV‐2 era: Is there any causative relation?
Authors: Pourbakhtyaran Elham , Heidari Morteza , Akbari Masood Ghahvechi , Mohammadi Mahmoud , Badv Reza Shervin , Zamani Gholamreza , Tavasoli Ali Reza , Rezaei Zahra , Mamishi Setareh , Haji Esmaeil Memar Elmira , Hosseiny Seyyed Mohammad Mahdi , Ghabeli Homa , Haghighi Roya , Ashrafi Mahmoud Reza 
Published: December 2022

AbstractWe reported an association between SARS‐CoV‐2 infection and Guillain–Barre syndrome (GBS). From 37 patients with GBS, previous SARS‐CoV‐2 clinical clues, including fever, cough, and diarrhea, were recorded in 18 patients. Among them, SARS‐CoV‐2 IgG was detected in seven patients, considered confirmed as cases. SARS‐CoV‐2 PCR was positive in just one patient. Although we found no increase in patient recruitment during the pandemic compared to previous years, our study indicated that SARS‐CoV‐2 is associated with poorer outcomes regarding GBS disability scale and hospital stay.
MOx@VOx‐Pd‐type Nanorods and Nanotubes as Catalysts for Selective Reduction of NO
Authors: Warmuth Lucas , Lott Patrick , Deutschmann Olaf , Feldmann Claus 
Published: December 2022

AbstractVanadium oxide (VOx‐Pd) nanotubes as well as VOx‐coated ZnO nanorods (ZnO@VOx‐Pd) and VOx‐coated, layered‐titania nanotubes (l‐TiO2@VOx‐Pd) are decorated with Pd nanoparticles and evaluated for selective catalytic reduction with hydrogen (H2‐SCR) for the first time. The nanostructures exhibit lengths of 300 to 700 nm, diameters of 20–100 nm and, in the case of the nanotubes, an inner tube diameter of about 10 nm. Pd nanoparticles (14±5 nm) are well‐dispersed over the respective nanorod/nanotube nanostructure. Structure and composition are characterized by SEM, TEM, EDXS with element mapping, XPS, FT‐IR, XRD, and sorption analysis. Thermal analysis indicates the nanostructures to be thermally stabile up to 350 °C (VOx), and 500 °C (ZnO@VOx, l‐TiO2@VOx). All catalysts are tested for their activity in regard of the selective catalytic reduction of NO with H2, revealing a significant impact of the catalyst support on both activity and selectivity. Specifically, l‐TiO2@VOx nanotubes show promising properties with an activity up to 70 % and a selectivity up to 80 % N2.
Bimetallic Catalysts for Sustainable Chemistry: Surface Redox Reactions For Tuning The Catalytic Surface Composition
Authors: Especel Catherine , Lafaye Gwendoline , Epron Florence 
Published: December 2022

AbstractThe development of a bimetallic catalyst for a given reaction requires not only the selection of the appropriate metals M1 and M2 but also the control as far as possible of the distribution of the two metals together and at the support surface in the case of supported catalysts. Preparation methods using redox reactions specifically enable the deposition of a second metal M2 at the surface of monometallic M1 nanoparticles, leading in most cases to core‐shell nanoparticles with strong metal‐metal interactions. Various methods are possible depending on the electrochemical potentials of the species involved: either a direct redox reaction, also named galvanic replacement, or the reduction of an intermediate reducing agent activated at the surface of M1. In this minireview, the fundamental bases of the preparation of bimetallic catalysts by both types of redox reactions and the recent advances in that domain are described.
Four‐Step Synthesis of (−)‐4‐epi‐Presilphiperfolan‐8α‐ol by Intramolecular Iron Hydride Atom Transfer‐Mediated Ketone‐Alkene Coupling and Studies to Accesstrans‐Hydrindanols with a Botryane Scaffold
Authors: Saladrigas Mar , Gómez‐Bengoa Enrique , Bonjoch Josep , Bradshaw Ben 
Published: December 2022

AbstractFrom an (R)‐(+)‐pulegone‐derived building block that incorporates the stereo‐defined tertiary carbon bearing a methyl group, as found in the targeted sesquiterpenoid, a four‐step synthesis of (−)‐4‐epi‐presilphiperfolan‐8‐α‐ol was achieved. The key processes involved are a ring‐closing metathesis leading to a bridged alkene‐tethered ketone and its subsequent FeIII‐mediated metal‐hydride atom transfer (MHAT) transannular cyclization. This synthetic method, implying an irreversible addition of a carbon‐centered radical upon a ketone by means of a hydrogen atom transfer upon the alkoxy radical intermediate, was also applied in the synthesis oftrans‐fused hydrindanols structurally related to botrydial compounds.
Vibrational Sum Frequency Generation Spectra of Water‐Vapor Interfaces Covered by Alcohols: Effects of Surface Coverage and Coupling between Oscillators
Authors: Das Banshi , Chandra Amalendu 
Published: December 2022

AbstractThe present study deals with the effects of varying coverage of water surface by alcohols on the vibrational sum frequency generation (VSFG) spectrum of interfacial water. We have considered two different alcohols: Tertiary butyl alcohol (TBA) whose alkyl part is fully branched and stearyl alcohol (STA) which has a long linear alkyl chain with larger hydrophobic surface area than that of TBA. With increase of the alcohol concentration, the hydrogen bonded OH stretch region of the VSFG spectrum is found to change following a regular trend for the STA‐water system, whereas non‐monotonic variation of the VSFG spectrum is observed for the TBA‐water system which can be correlated with the presence of very different interactions of TBA molecules at different concentrations. On increasing the concentration of TBA, the hydrophobic groups get more tilted towards the water phase and significant hydrophobic interactions are introduced at higher concentrations. Whereas, for STA, there is a gradual increase in the hydrophilic interaction. Because of stacking interactions between the long chain alkyl groups, the hydrophobic parts stay outward from the water phase at higher concentrations and a regular change in the VSFG spectrum is observed. We have also presented a computationally efficient scheme to calculate the VSFG spectrum of interfacial systems for coupled oscillators which is expected to be beneficial for the treatment of coupling where the interfacial system size is inherently large.
An ecosystems perspective on the reconversion of offshore platforms: Towards a multi‐level governance
Authors: Basile Vincenzo , Loia Francesca , Capobianco Nunzia , Vona Roberto 
Published: December 2022

AbstractThe decommissioning of offshore platforms has been increasingly discussed due to its economic, social, and environmental impacts. The high complexity of this multilevel context pushes for the adoption of a service ecosystem view to explore the value propositions and actors' relations involved in resource exchanges. This study follows a mixed‐method approach based on semistructured interviews conducted with oil and gas stakeholders and content analysis of the secondary data collected. The results highlight the ecosystem elements and identify the main drivers for sustainable growth in the process of the reconversion of oil and gas assets. A “meta” level is theorized to investigate how the actors' purposes can be harmonized with an ecosystem's goal to encourage the diffusion of a sustainable‐oriented culture in the context of offshore decommissioning. In this sense, the study provides several insights for researchers and professionals in both the local and national governance field and the oil and gas industry.
Synergistic Combination of Fermi Level Equilibrium and Plasmonic Effect for Formic Acid Dehydrogenation
Authors: Zhu Jiannan , Huang Jing , Dai Jiawei , Jiang Lipei , Xu You , Chen Rong , Li Longhua , Fu Xiaoqi , Wang Zhengyun , Liu Hongfang , Li Guangfang 
Published: December 2022

AbstractDeveloping an efficient catalyst for formic acid (FA) dehydrogenation is a promising strategy for safe hydrogen storage and transportation. Herein, we successfully developed trimetallic NiAuPd heterogeneous catalysts through a galvanic replacement reaction and a subsequent chemical reduction process to boost hydrogen generation from FA decomposition at room temperature by coupling Fermi level engineering with plasmonic effect. We demonstrated that Ni worked as an electron reservoir to donate electrons to Au and Pd driven by Fermi level equilibrium whereas plasmonic Au served as an optical absorber to generate energetic hot electrons and a charge‐redistribution mediator. Ni and Au worked cooperatively to promote the charge heterogeneity of surface‐active Pd sites, leading to enhanced chemisorption of formate‐related intermediates and eventually outstanding activity (342 mmol g−1 h−1) compared with bimetallic counterpart. This work offers excellent insight into the rational design of efficient catalysts for practical hydrogen energy exploitation.
Differences in the intrinsic chondrogenic potential of human mesenchymal stromal cells and iPSC‐derived multipotent cells
Authors: Xiang Shiqi , Lin Zixuan , Makarcyzk Meagan J. , Riewruja Kanyakorn , Zhang Yiqian , Zhang Xiurui , Li Zhong , Clark Karen L. , Li Eileen , Liu Silvia , Hao Tingjun , Fritch Madalyn R. , Alexander Peter G. , Lin Hang 
Published: December 2022

AbstractBackgroundHuman multipotent progenitor cells (hiMPCs) created from induced pluripotent stem cells (iPSCs) represent a new cell source for cartilage regeneration. In most studies, bone morphogenetic proteins (BMPs) are needed to enhance transforming growth factor‐β (TGFβ)‐induced hiMPC chondrogenesis. In contrast, TGFβ alone is sufficient to result in robust chondrogenesis of human primary mesenchymal stromal cells (hMSCs). Currently, the mechanism underlying this difference between hiMPCs and hMSCs has not been fully understood.MethodsIn this study, we first tested different growth factors alone or in combination in stimulating hiMPC chondrogenesis, with a special focus on chondrocytic hypertrophy. The reparative capacity of hiMPCs‐derived cartilage was assessed in an osteochondral defect model created in rats. hMSCs isolated from bone marrow were included in all studies as the control. Lastly, a mechanistic study was conducted to understand why hiMPCs and hMSCs behave differently in responding to TGFβ.ResultsChondrogenic medium supplemented with TGFβ3 and BMP6 led to robust in vitro cartilage formation from hiMPCs with minimal hypertrophy. Cartilage tissue generated from this new method was resistant to osteogenic transition upon subcutaneous implantation and resulted in a hyaline cartilage‐like regeneration in osteochondral defects in rats. Interestingly, TGFβ3 induced phosphorylation of both Smad2/3 and Smad1/5 in hMSCs, but only activated Smad2/3 in hiMPCs. Supplementing BMP6 activated Smad1/5 and significantly enhanced TGFβ’s compacity in inducing hiMPC chondrogenesis. The chondro‐promoting function of BMP6 was abolished by the treatment of a BMP pathway inhibitor.ConclusionsThis study describes a robust method to generate chondrocytes from hiMPCs with low hypertrophy for hyaline cartilage repair, as well as elucidates the difference between hMSCs and hiMPCs in response to TGFβ. Our results also indicated the importance of activating both Smad2/3 and Smad1/5 in the initiation of chondrogenesis.
Loss of G0/G1 switch gene 2 (G0S2) promotes disease progression and drug resistance in chronic myeloid leukaemia (CML) by disrupting glycerophospholipid metabolism
Authors: Gonzalez Mayra A. , Olivas Idaly M. , Bencomo‐Alvarez Alfonso E. , Rubio Andres J. , Barreto‐Vargas Christian , Lopez Jose L. , Dang Sara K. , Solecki Jonathan P. , McCall Emily , Astudillo Gonzalo , Velazquez Vanessa V. , Schenkel Katherine , Reffell Kelaiah , Perkins Mariah , Nguyen Nhu , Apaflo Jehu N. , Alvidrez Efren , Young James E. , Lara Joshua J. , Yan Dongqing , Senina Anna , Ahmann Jonathan , Varley Katherine E. , Mason Clinton C. , Eide Christopher A. , Druker Brian J. , Nurunnabi Md , Padilla Osvaldo , Bajpeyi Sudip , Eiring Anna M. 
Published: December 2022

AbstractTyrosine kinase inhibitors (TKIs) targeting BCR::ABL1 have turned chronic myeloid leukaemia (CML) from a fatal disease into a manageable condition for most patients. Despite improved survival, targeting drug‐resistant leukaemia stem cells (LSCs) remains a challenge for curative CML therapy. Aberrant lipid metabolism can have a large impact on membrane dynamics, cell survival and therapeutic responses in cancer. While ceramide and sphingolipid levels were previously correlated with TKI response in CML, the role of lipid metabolism in TKI resistance is not well understood. We have identified downregulation of a critical regulator of lipid metabolism, G0/G1 switch gene 2 (G0S2), in multiple scenarios of TKI resistance, including (1) BCR::ABL1 kinase‐independent TKI resistance, (2) progression of CML from the chronic to the blast phase of the disease, and (3) in CML versus normal myeloid progenitors. Accordingly, CML patients with low G0S2 expression levels had a worse overall survival. G0S2 downregulation in CML was not a result of promoter hypermethylation or BCR::ABL1 kinase activity, but was rather due to transcriptional repression by MYC. Using CML cell lines, patient samples and G0s2 knockout (G0s2−/−) mice, we demonstrate a tumour suppressor role for G0S2 in CML and TKI resistance. Our data suggest that reduced G0S2 protein expression in CML disrupts glycerophospholipid metabolism, correlating with a block of differentiation that renders CML cells resistant to therapy. Altogether, our data unravel a new role for G0S2 in regulating myeloid differentiation and TKI response in CML, and suggest that restoring G0S2 may have clinical utility.
Artemisitene suppresses rheumatoid arthritis progression via modulating METTL3‐mediated N6‐methyladenosine modification of ICAM2 mRNA in fibroblast‐like synoviocytes
Authors: Chen Jian , Lin Xian , He Juan , Liu Dandan , He Lianhua , Zhang Miaomiao , Luan Huijie , Hu Yiping , Tao Cheng , Wang Qingwen 
Published: December 2022

AbstractBackgroundRheumatoid arthritis (RA) is a chronic autoimmune disease. We previously revealed that the natural compound artemisitene (ATT) exhibits excellent broad anticancer activities without toxicity on normal tissues. Nevertheless, the effect of ATT on RA is undiscovered. Herein, we aim to study the effect and potential mechanism of ATT on RA management.MethodsA collagen‐induced arthritis (CIA) mouse model was employed to confirm the anti‐RA potential of ATT. Cell Counting Kit‐8 (CCK‐8) and 5‐ethynyl‐2'‐deoxyuridine (EdU) assays, cell cycle and apoptosis analysis, immunofluorescence, migration and invasion assays, quantitative real‐time PCR (RT‐qPCR), Western blot, RNA‐sequencing (RNA‐seq) analysis, plasmid construction and lentivirus infection, and methylated RNA immunoprecipitation and chromatin immunoprecipitation assays, were carried out to confirm the effect and potential mechanism of ATT on RA management.ResultsATT relieved CIA in mice. ATT inhibited proliferation and induced apoptosis of RA‐fibroblast‐like synoviocytes (FLSs). ATT restrained RA‐FLSs migration and invasion via suppressing epithelial–mesenchymal transition. RNA‐sequencing analysis and bioinformatics analysis identified intercellular adhesion molecule 2 (ICAM2) as a promoter of RA progression in RA‐FLSs. ATT inhibits RA progression by suppressing ICAM2/phosphoinositide 3‐kinase (PI3K)/protein kinase B (AKT)/p300 pathway in RA‐FLSs. Moreover, ATT inhibited methyltransferase‐like 3 (METTL3)‐mediated N6‐methyladenosine methylation of ICAM2 mRNA in RA‐FLSs. Interestingly, p300 directly facilitated METTL3 transcription, which could be restrained by ATT in RA‐FLSs. Importantly, METTL3, ICAM2 and p300 expressions in synovium tissues of RA patients were related to clinical characteristics and therapy response.ConclusionsWe provided strong evidence that ATT has therapeutic potential for RA management by suppressing proliferation, migration and invasion, in addition to inducing apoptosis of RA‐FLSs through modulating METTL3/ICAM2/PI3K/AKT/p300 feedback loop, supplying the fundamental basis for the clinical application of ATT in RA therapy. Moreover, METTL3, ICAM2 and p300 might serve as biomarkers for the therapy response of RA patients.
Flow visualization simulation of cemented tailings backfill slurry by particle tracking technology
Authors: Sun Haikuan , Gan Deqing , Xue Zhenlin , Zhang Yajie 
Published: December 2022

AbstractPipeline flow visualization of cemented tailings backfill slurry (CTBS) improves the safety and stability of transportation. High turbidity and low resolution make it difficult for conventional methods to monitor the particle distribution state of CTBS in a short period of time. Particle tracking technology (PTT) is used to simulate and investigate the flow characteristics of CTBS pipeline, combine with theoretical analysis to construct a CTBS pipeline visualization model, elaborate the particle distribution state when CTBS flows in the pipeline, and explore the effects of pipe diameter (PD), flow velocity (FV) and tailings gradation (TG) on the particle distribution. The results show that particle tracking technology is better applied to investigate the particle transport distribution characteristics of CTBS tailings. Three concepts of particle accumulated gravity Ga, static friction angle θ and diameter dividing line are defined, and the transport pipe is divided into light wear zone, medium wear zone and heavy wear zone. The increase in pipe diameter increases the content of fine particles at the pipe wall and the thickness of the lubrication layer becomes larger, which improves the safety and stability of CTBS transport. The increased flow velocity reduces the settling phenomenon of large size particles and improves the transport efficiency, which increases the pipeline transport resistance. Under good tailings grading conditions, a wide range of tailings grading is more suitable as backfill material. The results of the study illustrate the flow characteristics of the backfill slurry in the pipeline from the particle perspective and provide a theoretical basis for pipeline transportation research.