bluuebunny/crossref_metadata_2025_split huggingface dataset:
Health promotion programs for middle‐aged adults that promote physical activity or healthy eating and involve local governments and health services: A rapid review
Authors: Wallbank Geraldine , Voukelatos Alexander , Taki Sarah , Hughes Jessica K. , Gammack Stephen , Pokhrel Ruby , Bedford Karen , Simone Lisa , Wen Li Ming 
Published: February 2023

AbstractBackgroundNoncommunicable diseases can be prevented or delayed through health promotion programs. Little is known about programs delivered by partnership organisations that address lifestyle behaviours. The study's purpose was to review the literature on physical activity or healthy eating health promotion programs, delivered in partnership by the local government and local health services, to describe characteristics of programs and their impact on physical activity, healthy eating or related health outcomes among middle‐aged adults.MethodsThis rapid review was conducted from November 2021 to June 2022, informed by the Cochrane Rapid Reviews Methods guidance for conducting rapid reviews. Articles published in English since 2000 were identified in Medline, Embase, CINAHL, AgeLine and Scopus databases. A narrative synthesis was performed.ResultsTen articles involving 19 802 participants were identified from a total of 4847 articles identified from the search. The primary role of the partnership was providing funds. Other roles were facilitating stakeholder involvement, program development, delivery and recruitment. Positive outcomes were likely if programs were developed by collaborative stakeholder partnerships, informed by previous research or a behaviour change framework. The heterogeneity of study designs and reported outcomes did not permit meta‐analysis.ConclusionThis review highlights the lack of evidence of local government‐health service partnerships delivering physical activity or healthy eating health promotion programs for middle‐aged adults. Programs designed collaboratively with an evidence base or a theory base are recommended and can guide future work investigating strategies for partnership development.So What?Physical activity or healthy eating health promotion programs need early stakeholder collaborative input designed with a theory/evidence base. This can guide future work for investigating strategies for partnership development.
Effect of iron on biological clogging in porous media: Implications for managed aquifer recharge
Authors: Cui Ruijuan , Page Declan , Du Xinqiang , Zhang Hexuan , Ye Xueyan 
Published: February 2023

AbstractManaged aquifer recharge (MAR) is increasingly being adopted to improve water security internationally. However, clogging during MAR remains one of the greatest challenges for sustainable operations. This study examines the effects of iron on biological clogging processes using column experiments and suggests management options. The results indicated that the presence of iron limits the transport of bacteria through the column, and that concentrations <10 mg/L are correlated with increased bacterial growth. Conversely, the increased viscosity of biofilm subsequently limits the transport of iron through the column. Fourier transform infrared spectroscopy and x‐ray photoelectron spectroscopy indicated that large iron‐Pseudomonas sp. flocs formed which occupied the sand pore spaces. The effect of iron induced chemical clogging was most notable in the initial stage of the experiment while bio‐clogging dominated later. There are many recommended values of iron concentration in water recharge, most of them are advised from the point of pollution perspective. Based on these laboratory results, iron concentrations in recharge water for MAR should be <0.3 mg/L to mitigate clogging effects. Furthermore, using non‐corrodible materials for bore screen and pumps, and avoiding external oxidant inputs should be considered to prevent iron related chemical and biological clogging.
Toxicology assessment for six per- and polyfluoroalkyl (PFAS)-free aqueous film forming foam (AFFF) products
Authors: Holden Lindsay A. , East Andrew G. , Narizzano Allison M. , Quinn Michael J. 
Published: February 2023

Abstract
There is an urgent need to understand the toxicity hazards of aqueous film forming foam (AFFF) replacement products to ensure the balance between performance and toxicity hazards and avoid regrettable substitutions during the rapid phasing out of per- and polyfluoroalkyl substance (PFAS)-containing AFFFs. To address this need, we assessed the toxicity of six candidate PFAS-free products via literature review, estimation techniques, and incorporation of testing data from whole products and compared them against one PFAS-containing product. Then, we combined the relative hazards across human occupational exposure (e.g., concentrate, foam, or dilute exposures), human environmental exposure (e.g., training, emergency response, cleanup), and environmental exposure to aquatic, mammalian, and other terrestrial species using an index-based scoring system to quantify potential hazards across these domains. We found that most PFAS-free products in their concentrated form may cause dermal and ocular irritation, and aquatic toxicity may be a concern from direct or repeated environmental releases. Additionally, all PFAS-free AFFF products assessed contain chemicals that are notable as plausible hazards resulting from release uncertainties (e.g., concentration, release volume, release timing), but the PFAS-free AFFF products appear to have a lower likelihood of environmental persistence and bioaccumulation and to have lower oral human health toxicity than the PFAS-containing reference product. Decision makers can use this information alongside cost–benefit, sustainability, or life-cycle analyses to make a data-driven decision for the adoption of PFAS-free AFFF. Integr Environ Assess Manag 2023;19:1609–1618. © 2023 SETAC
A reflex testing protocol using two multivariate index assays improves the risk assessment for ovarian cancer in patients with an adnexal mass
Authors: Fritsche Herbert A. , Bullock Rowan G. 
Published: February 2023

AbstractObjectivesPatients with adnexal masses suspicious for malignancy benefit from referral to oncology specialists during presurgical assessment of the mass. OVA1 is a multivariate assay using a five‐biomarker panel which offers high overall and early‐stage sensitivity. However, OVA1 has a high false‐positive rate for benign masses. Overa, a second‐generation multivariate index assay was developed to reduce the false‐positive rate. The aim of the present study was to use Overa as a reflex for OVA1 and increase specificity.MethodsOVA1 cut‐off scores were established to place patients into three categories: low, intermediate, and high cancer risk. Samples with intermediate‐risk OVA1 scores were reflexed to the Overa and defined as high or low risk. This protocol was tested with 1035 prospectively collected serum samples and validated with an independent prospectively collected sample set (N = 207).ResultsThirty‐five per cent (359) of samples had intermediate OVA1 scores. Reflexing these to Overa eliminated 58% of the false‐positives and improved the overall specificity from 50% to 72%. This finding was confirmed in the independent dataset, in which the specificity increased from 56% to 73%.ConclusionsReflexing samples with intermediate OVA1 scores significantly decreases the false‐positive rate, thereby reducing unnecessary surgical referrals.
Question 63: What role is played by multidisciplinary dialogue to achieve sustainable development goals?
Authors: Makin Ian W. , Pearson Damien , Ravi Varun 
Published: February 2023

AbstractCongress Question 63 ‘What role is played by multidisciplinary dialogue to achieve sustainable development goals?’ sought to explore how a multidisciplinary approach can be enhanced with improved interactions and new layers to engage in the attainment of sustainable development goals (SDGs) more effectively. In addressing these questions, some 39 papers summarized findings from about 24 countries representing substantially different economic situations, climate, farming systems and water resources. The papers illustrated the potential of irrigated agriculture to help end poverty (SDG 1), end hunger (SDG 2), to promote collaboration and investments (SDG 17) and sustain water‐related ecosystems (SDG 14 and SDG 15). The papers highlight the need for increased, and improved, exchange of information among multiple stakeholders involved in agricultural production systems, from field level, through the supply chain, to the consumers of the food and fibre created. Furthermore, a continued focus on capacity development among stakeholders in the sector is essential with improved communication of the value and challenges of agricultural water management beyond the immediate stakeholders (farmers, system operators, water sector regulators, etc.). As greater attention is directed towards the role of agricultural water use in the management of water resources and related ecosystems, the irrigation and drainage profession must communicate more effectively with the broader society to maintain a ‘social license to operate’.
Development and test of highly accurate endpoint free energy methods. 2: Prediction of logarithm of n‐octanol–water partition coefficient (logP) for druglike molecules using MM‐PBSA method
Authors: Sun Yuchen , Hou Tingjun , He Xibing , Man Viet Hoang , Wang Junmei 
Published: February 2023

AbstractThe logarithm of n‐octanol–water partition coefficient (logP) is frequently used as an indicator of lipophilicity in drug discovery, which has substantial impacts on the absorption, distribution, metabolism, excretion, and toxicity of a drug candidate. Considering that the experimental measurement of the property is costly and time‐consuming, it is of great importance to develop reliable prediction models for logP. In this study, we developed a transfer free energy‐based logP prediction model‐FElogP. FElogP is based on the simple principle that logP is determined by the free energy change of transferring a molecule from water to n‐octanol. The underlying physical method to calculate transfer free energy is the molecular mechanics‐Poisson Boltzmann surface area (MM‐PBSA), thus this method is named as free energy‐based logP (FElogP). The superiority of FElogP model was validated by a large set of 707 structurally diverse molecules in the ZINC database for which the measurement was of high quality. Encouragingly, FElogP outperformed several commonly‐used QSPR or machine learning‐based logP models, as well as some continuum solvation model‐based methods. The root‐mean‐square error (RMSE) and Pearson correlation coefficient (R) between the predicted and measured values are 0.91 log units and 0.71, respectively, while the runner‐up, the logP model implemented in OpenBabel had an RMSE of 1.13 log units and R of 0.67. Given the fact that FElogP was not parameterized against experimental logP directly, its excellent performance is likely to be expanded to arbitrary organic molecules covered by the general AMBER force fields.
Tight junctions and acute kidney injury
Authors: Wei Wei , Li Weiying , Yang Letian , Weeramantry Savidya , Ma Liang , Fu Ping , Zhao Yuliang 
Published: February 2023

AbstractAcute kidney injury (AKI) is characterized by a rapid reduction in kidney function caused by various etiologies. Tubular epithelial cell dysregulation plays a pivotal role in the pathogenesis of AKI. Tight junction (TJ) is the major molecular structure that connects adjacent epithelial cells and is critical in maintaining barrier function and determining the permeability of epithelia. TJ proteins are dysregulated in various types of AKI, and some reno‐protective drugs can reverse TJ changes caused by insult. An in‐depth understanding of TJ regulation and its causality with AKI will provide more insight to the disease pathogenesis and will shed light on the potential role of TJs to serve as novel therapeutic targets in AKI.
Regulation of epidermal stratification and development by basal keratinocytes
Authors: Yin Hanxiao , Hu Mingzheng , Li Dengwen 
Published: February 2023

AbstractThe epidermis is a stratified squamous epithelium distributed in the outermost layer of the skin and is intimately involved in the formation of a physical barrier to pathogens. Basal keratinocytes possess the properties of stem cells and play an essential role in epidermal development and skin damage recovery. Therefore, understanding the molecular mechanism of how basal keratinocytes participate in epidermal development and stratification is vital for preventing and treating skin lesions. During epidermal morphogenesis, the symmetric division of basal keratinocytes contributes to the extension of skin tissues, while their asymmetric division and migration facilitate epidermal stratification. In this review, we summarize the process of epidermal stratification and illustrate the molecular mechanisms underlying epidermal morphogenesis. Furthermore, we discuss the coordination of multiple signaling pathways and transcription factors in epidermal stratification, together with the roles of cell polarity and cell dynamics during the process.
Whole‐genome sequencing of Crimean−Congo hemorrhagic fever virus circulating in Pakistan during 2022
Authors: Umair Massab , Rehman Zaira , Haider Syed A. , Ali Qasim , Hakim Rabia , Bibi Shaheen , Salman Muhammad , Ikram Aamer 
Published: February 2023

AbstractPakistan is an endemic country for Crimean−Congo hemorrhagic fever (CCHF) and its Balochistan province is considered a hotspot region for circulation of the virus whereas sporadic cases have been reported from other parts of the country. Our study aims to investigate the genomic diversity of the CCHF virus circulating in Punjab and Khyber Pakhtunkhwa provinces of Pakistan. Between April to September 2022, 46 samples from suspected CCHF patients were tested, with 6 (13%) showing positive RT‐PCR results. Among the positive cases, all were male, aged 14−48 years among which 4 were butchers. Three CCHF patients succumbed to the disease. The complete S‐M‐L‐gene fragments of 4 positive samples were sequenced. The S and L segments belonged to the Asia‐1 clade and clustered with regional strains from Iran, India, and Afghanistan. One M segment sequence grouped into Africa‐2 along with those reported from India during 2016−2019. We report the detection of a reassorted virus (NIH‐PAK‐CCHF‐03|2022) having Asia‐1‐Africa‐2‐Asia‐1 (S‐M‐L) segment pattern for the first time from Pakistan. Mutational analysis showed M segments harboring eight mutations (T55A, S80P, T110I, T185A, T189A, A212T, and N239I/T) in the mucin‐like domain, five mutations (D250N, T333S, I375V, M401I, A433T), four mutations (N545D, Y657F, K688R, and I824V) in GP38‐domain, and three mutations (T1418N, A1431V, and G1449S) in Gc‐domain. These findings highlight the significance of whole‐genome sequencing of indigenous strains for a better understanding of the CCHFV evolution in Pakistan.
Integrative review of remote patient monitoring in gynecologic and urologic surgical oncology
Authors: Perez Fernanda , Nolde Margaret , Crane Tracy E. , Kebria Mehdi , Chan Kevin , Dellinger Thanh , Sun Virginia 
Published: February 2023

AbstractPatients with cancer facing complex and invasive urologic and gynecologic cancer surgery often experience symptoms and rapid declines in functional capacity postoperatively. Remote patient monitoring that leverages patient‐generated health data is a potential approach to assess and promote postoperative recovery. This integrative review aims to provide an overview of the current literature and research on remote patient monitoring in gynecologic and urologic surgical oncology.
Developing an Objective Structured Assessment of Technical Skills (OSATS) for Microlaryngoscopy
Authors: Ramazani Fatemeh , Wright Erin D , Randall Derrick R , Lin Jun R , Jeffery Caroline C 
Published: February 2023

BackgroundMicrolaryngoscopy is a basic technical skill in Oto‐HNS. It is essential for residency programs to have a competency‐based assessment tool to evaluate residents' performance of this procedure. An Objective Structured Assessment of Technical Skills (OSATS) is a procedure‐specific assessment, which consists of the following: (a) Operation‐Specific Checklist and (b) Global Rating Scale (GRS).ObjectiveThe objective of this study was to create an OSATS for adult microlaryngoscopy.MethodsThis was a prospective study, with an initial qualitative phase for OSATS development (Phase I), and a clinical pilot phase (Phase II). In Phase I, interviews were conducted with three laryngologists to establish a stepwise description of adult microlaryngoscopy and review a previously validated GRS for relevance to microlaryngoscopy. Responses were used to create a framework for the OSATS. The OSATS was then presented to Oto‐HNS residents and laryngologists in an alternating fashion, for review of clarity and relevance. A pilot study was then performed to evaluate the resident performance of adult microlaryngoscopy. Multiple regression analysis was carried out to investigate whether training level, case complexity, and previous OSATS exposure could predict participant scores.ResultsPhase I of this study led to the creation of a 34‐item OSATS. The pilot study (N = 28 procedures) revealed that training level was significantly correlated with increased OSATS scores. There was no statistically significant correlation between case complexity and resident scores. Assessors reported the perceived utility of the OSATS and intent for use in residency training.ConclusionApplication of the proposed OSATS will allow for competency‐based assessment of the resident performance of microlaryngoscopy.Level of EvidenceNA Laryngoscope, 133:2719–2724, 2023
Comparison of CPAP and Expansion Sphincter Pharyngoplasty using the Mean Disease Alleviation Concept
Authors: Fiorella Michele , Armache Maria , Scott Elizabeth , Rodin Julianna , Boon Maurits , Huntley Colin 
Published: February 2023

ObjectiveContinuous Positive Airway Pressure (CPAP) is the typical first treatment for Obstructive Sleep Apnea (OSA); however, patient adherence is often suboptimal. Expansion Sphincter Pharyngoplasty (ESP) is an alternative treatment option for patients with OSA who demonstrate signs of palatal and lateral pharyngeal collapse. The aim of this study is to compare therapeutic outcomes, using the mean disease alleviation concept, for patients who underwent ESP to patients undergoing CPAP therapy.Data SourcesSingle‐institution retrospective cohort study.MethodsAll patients who underwent ESP from 2018 to 2021 or were prescribed CPAP from December to June 2021 at our institution were assessed for inclusion. ESP patients who had pre‐ and post‐operative sleep studies available and CPAP patients who followed up at our institution's sleep clinic were included for analysis. Charts were reviewed for demographic information and sleep study results, and treatment outcomes were measured by calculating mean disease alleviation.ResultsThere were 77 patients in the ESP group and 107 patients in the CPAP group. AHI reduction was greater in the CPAP group (p = 0.016); however, mean disease alleviation was similar between groups (p = 0.076). One‐way ANCOVA demonstrated similar MDA between groups when controlling for patient age, BMI, gender, and pre‐operative AHI (F [1,177] = 2,931.6, p = 0.104).ConclusionCPAP therapy provided superior reduction in AHI compared to ESP; however, overall treatment efficacy as measured by mean disease alleviation was similar for both groups.Level of Evidence3 Laryngoscope, 133:1513–1517, 2023
Small‐organelle‐enriched metagenomics: An improved method for environmental DNA‐based identification of marine plankton
Authors: Jin Soyeong , Lee Hyun‐Gwan , Park Chungoo , Kim Kwang Young 
Published: February 2023

AbstractUnderstanding marine biodiversity is of paramount importance. Recently, we developed a polymerase chain reaction (PCR)‐free small‐organelle‐enriched metagenomics (SoEM) method with enhanced performance in species identification compared to multimarker DNA metabarcoding. However, this method has a problem of applicability for field surveys, for example, the need to filter large volumes of seawater. Here, we propose an improved version of the original SoEM method (SoEM pioneer version [SoEM‐pv]) for marine biodiversity assessment. There are three major contributions to this proposed SoEM method (SoEM v1.0): (1) presentation of PCR‐free protocols for seawater environmental DNA analysis; (2) provision of widely applicable optimal DNA extraction protocols that can be applied to small volumes (approximately 2 liters) of coastal waters; and (3) description of easy‐to‐use bioinformatics pipelines for analysis of the SoEM data. To validate the accuracy and sensitivity of SoEM v1.0, we evaluated the performance of the taxonomic capture capacity from seawater samples and found that, given the sample volume of 2 liters, (1) SoEM v1.0 had an approximately 5‐ to 10‐fold higher DNA extraction yield than the SoEM‐pv; (2) SoEM v1.0 had the highest taxonomic diversity compared to the other methods, 1.6‐fold higher than PCR‐based multi‐locus DNA metabarcoding and 1.2‐fold higher than shotgun metagenomic sequencing; and (3) the number of species identified did not increase proportionally with increasing sample size from 2 to 20 liters. We suggest that SoEM v1.0 is a promising and feasible method for marine eukaryotic diversity studies and expect it to be welcomed by biological oceanographers and coastal resource managers.
Photobiomodulation therapy mitigates cardiovascular aging and improves survival
Authors: Syed Sunayana Begum , Ahmet Ismayil , Chakir Khalid , Morrell Christopher H. , Arany Praveen R. , Lakatta Edward G. 
Published: February 2023

AbstractBackgroundPhotobiomodulation (PBM) therapy, a form of low‐dose light therapy, has been noted to be effective in several age‐associated chronic diseases such as hypertension and atherosclerosis. Here, we examined the effects of PBM therapy on age‐associated cardiovascular changes in a mouse model of accelerated cardiac aging.MethodsFourteen months old Adenylyl cyclase type VIII (AC8) overexpressing transgenic mice (n = 8) and their wild‐type (WT) littermates (n = 8) were treated with daily exposure to Near‐Infrared Light (850 nm) at 25 mW/cm2 for 2 min each weekday for a total dose of 1 Einstein (4.5 p.J/cm2 or fluence 3 J/cm2) and compared to untreated controls over an 8‐month period. PBM therapy was administered for 3.5 months (Early Treatment period), paused, due to Covid‐19 restrictions for the following 3 months, and restarted again for 1.5 months. Serial echocardiography and gait analyses were performed at monthly intervals, and serum TGF‐β1 levels were assessed following sacrifice.ResultsDuring the Early Treatment period PBM treatments: reduced the age‐associated increases in left ventricular (LV) mass in both genotypes (p = 0.0003), reduced the LV end‐diastolic volume (EDV) in AC8 (p = 0.04); and reduced the left atrial dimension in both genotypes (p = 0.02). PBM treatments substantially increased the LV ejection fraction (p = 0.03), reduced the aortic wall stiffness (p = 0.001), and improved gait symmetry, an index of neuro‐muscular coordination (p = 0.005). The effects of PBM treatments, measured following the pause, persisted. Total TGF‐β1 levels were significantly increased in circulation (serum) in AC8 following PBM treatments (p = 0.01). We observed a striking increase in cumulative survival in PBM‐treated AC8 mice (100%; p = 0.01) compared to untreated AC8 mice (43%).ConclusionPBM treatment mitigated age‐associated cardiovascular remodeling and reduced cardiac function, improved neuromuscular coordination, and increased longevity in an experimental animal model. These responses correlate with increased TGF‐β1 in circulation. Future mechanistic and dose optimization studies are necessary to assess these anti‐aging effects of PBM, and validation in future controlled human studies is required for effective clinical translation.