bluuebunny/crossref_metadata_2025_split huggingface dataset:
Fe
3+
-binding transferrin nanovesicles encapsulating sorafenib induce ferroptosis in hepatocellular carcinoma
Authors: Xiao Youmei , Xu Zhanxue , Cheng Yuan , Huang Rufan , Xie Yuan , Tsai Hsiang-i , Zha Hualian , Xi Lifang , Wang Kai , Cheng Xiaoli , Gao Yanfeng , Zhang Changhua , Cheng Fang , Chen Hongbo 
Published: June 2023

Abstract

Background
Ferroptosis, iron-dependent cell death, is an established mechanism for cancer suppression, particularly in hepatocellular carcinoma (HCC). Sorafenib (SOR), a frontline drug for the treatment of HCC, induces ferroptosis by inhibiting the Solute Carrier family 7 member 11 (SLC7A11), with inadequate ferroptosis notably contributing to SOR resistance in tumor cells.

Methods

To further verify the biological targets associated with ferroptosis in HCC, an analysis of the Cancer Genome Atlas (TCGA) database was performed to find a significant co-upregulation of SLC7A11 and transferrin receptor (TFRC), Herein, cell membrane-derived transferrin nanovesicles (TF NVs) coupled with Fe
3+
and encapsulated SOR (SOR@TF-Fe
3+
NVs) were established to synergistically promote ferroptosis, which promoted the iron transport metabolism by TFRC/TF-Fe
3+
and enhanced SOR efficacy by inhibiting the SLC7A11.

Results

In vivo and in vitro experiments revealed that SOR@TF-Fe
3+
NVs predominantly accumulate in the liver, and specifically targeted HCC cells overexpressing TFRC. Various tests demonstrated SOR@TF-Fe
3+
NVs accelerated Fe
3+
absorption and transformation in HCC cells. Importantly, SOR@TF-Fe
3+
NVs were more effective in promoting the accumulation of lipid peroxides (LPO), inhibiting tumor proliferation, and prolonging survival rates in HCC mouse model than SOR and TF- Fe
3+
NVs alone.

Conclusions
The present work provides a promising therapeutic strategy for the targeted treatment of HCC.

Graphical abstract
Development of 3D printed patient-specific skull implants based on 3d surface scans
Authors: Kropla Fabian , Winkler Dirk , Lindner Dirk , Knorr Patrick , Scholz Sebastian , Grunert Ronny 
Published: June 2023

AbstractSometimes cranioplasty is necessary to reconstruct skull bone defects after a neurosurgical operation. If an autologous bone is unavailable, alloplastic materials are used. The standard technical approach for the fabrication of cranial implants is based on 3D imaging by computed tomography using the defect and the contralateral site. A new approach uses 3D surface scans, which accurately replicate the curvature of the removed bone flap. For this purpose, the removed bone flap is scanned intraoperatively and digitized accordingly. When using a design procedure developed for this purpose creating a patient-specific implant for each bone flap shape in short time is possible. The designed skull implants have complex free-form surfaces analogous to the curvature of the skull, which is why additive manufacturing is the ideal manufacturing technology here. In this study, we will describe the intraoperative procedure for the acquisition of scanned data and its further processing up to the creation of the implant.
Malondialdehyde (MDA) and 8-hydroxy-2′-deoxyguanosine (8-OHdG) in ischemic stroke: a systematic review
Authors: Tugasworo Dodik , Prasetyo Awal , Kurnianto Aditya , Retnaningsih Retnaningsih , Andhitara Yovita , Ardhini Rahmi , Budiman Jethro 
Published: June 2023

Abstract
Introduction
The low blood flow in ischemic stroke reduces oxygen and glucose and induces a series of reactions that produce free radicals. Free radicals can destroy cell membranes (lipid peroxidation) marked by the elevation of malondialdehyde (MDA), and the damage of deoxyribonucleic acid (DNA) showed by the elevation of 8-hydroxy-2’-deoxyguanosine (8-OHdG). This review aimed to assess and conclude the research-based study systematically to analyze the relationship of MDA/8-OHdG and ischemic stroke.

Method
Cochrane handbook for systematic reviews, the guideline of preferred reporting items for systematic review and meta-analysis (PRISMA), and synthesis without meta-analysis (SWiM) in systematic reviews: reporting guideline was used as guideline in this systematic review. Inclusion criteria in this review were primary studies of every design, articles published in English around January 2000–December 2021, and the study used human as subject. A systematic literature search was applied in 15 electronic medical journals. The authors assessed the study quality and risk of bias of each included study.

Results
The authors evaluated 374 studies found in literature searching, 30 studies met the criteria for this review, and then underwent the assessment of study quality and risk of bias.

Conclusion
MDA has the role as oxidative stress biomarker, outcome predictor, mortality predictor, post-stroke cognitive impairment predictor, post-stroke depression predictor, and hand grip strength predictor of ischemic stroke; while 8-OHdG has the role as oxidative stress biomarker, outcome predictor, mortality predictor, post-stroke cognitive impairment predictor, post-stroke depression predictor of ischemic stroke.
Clinical importance of peri-ictal water drinking
Authors: Al-Attas Alawi A. 
Published: June 2023

Abstract
Background
Peri-ictal water drinking is drinking water within a short period or during a seizure. This behavior can be experienced in childhood and adulthood and commonly affects adults suffering from temporal lobe epilepsy. Peri-ictal water drinking has clinical importance for lateralizing signs in the non-dominant hemisphere. It has been found in up to 7–15% of patients with focal epilepsy.

Case presentation
This case study involved a 44-year-old right-handed female referred tour center as a case of drug-resistant epilepsy for presurgical evaluation. After evaluation in the Epilepsy Monitoring Unit, the patient was considered a candidate for right temporal lobectomy. The patient exhibited good outcome post-temporal lobectomy.

Conclusions
This case highlights the previously observed association between peri-ictal water drinking and the non-dominant hemisphere in patients with epilepsy. Clinicians must not overlook this automatic behavior that both patients and physicians usually underestimate because drinking water is a normal phenomenon.
Genetic tool used to diagnose achromatopsia: first case report from India
Authors: Pritti Kumari , Mishra Vineet , Kotecha Udhaya , Aggarwal Somesh 
Published: June 2023

Abstract
Background
Achromatopsia is an autosomal recessive disease characterized by poor visual acuity, lack of color vision, nystagmus, and marked photophobia. The symptoms can be extremely disabling, and at present, there is no cure available. Mutations in the CNGA3, CNGB3, GNAT2, PDE6C, PDE6H, and ATF6 genes have been identified as associated with this disease. The genetic approach for these patients is currently an important issue, and gene therapy is an ongoing therapeutic option already being studied in clinical trials.

Case presentation
We report the case of two siblings (8 and 5 years old) affected by achromatopsia. They carry compound heterozygous mutations in the CNGA3 gene at positions 1306 and 1279 in exon 8 (c.1306C > T, p.Arg436Trp and c.1279C > T, p.Arg427Cys). The parents were found to have one of the variants in a heterozygous condition. This is the first reported case of achromatopsia in India.

Conclusion
This report emphasizes the importance of genetic testing in such patients, which can aid not only in the diagnosis and management but also in providing counseling to parents regarding the significance of prenatal diagnosis in future pregnancies, helping them make informed choices. Due to its variable presentation, the diagnosis of achromatopsia may be challenging, and exome sequencing has proven to be a crucial diagnostic tool.
International Tuberculum Sellae Meningioma Study: Surgical Outcomes and Management Trends
Authors: Magill Stephen T. , Schwartz Theodore H. , Couldwell William T. , Gardner Paul A. , Heilman Carl B. , Sen Chandranath , Akagami Ryojo , Cappabianca Paolo , Prevedello Daniel M. , McDermott Michael W. 
Published: June 2023

BACKGROUND AND OBJECTIVES:
Tuberculum sellae meningiomas (TSMs) can be resected through transcranial (TCA) or expanded endonasal approach (EEA). The objective of this study was to report TSM management trends and outcomes in a large multicenter cohort.

METHODS:
This is a 40-site retrospective study using standard statistical methods.

RESULTS:
In 947 cases, TCA was used 66.4% and EEA 33.6%. The median maximum diameter was 2.5 cm for TCA and 2.1 cm for EEA (P < .0001). The median follow-up was 26 months. Gross total resection (GTR) was achieved in 70.2% and did not differ between EEA and TCA (P = .5395). Vision was the same or better in 87.5%. Vision improved in 73.0% of EEA patients with preoperative visual deficits compared with 57.1% of TCA patients (P < .0001). On multivariate analysis, a TCA (odds ratio [OR] 1.78, P = .0258) was associated with vision worsening, while GTR was protective (OR 0.37, P < .0001). GTR decreased with increased diameter (OR: 0.80 per cm, P = .0036) and preoperative visual deficits (OR 0.56, P = .0075). Mortality was 0.5%. Complications occurred in 23.9%. New unilateral or bilateral blindness occurred in 3.3% and 0.4%, respectively. The cerebrospinal fluid leak rate was 17.3% for EEA and 2.2% for TCA (OR 9.1, P < .0001). The recurrence rate was 10.9% (n = 103). Longer follow-up (OR 1.01 per month, P < .0001), World Health Organization II/III (OR 2.20, P = .0262), and GTR (OR: 0.33, P < .0001) were associated with recurrence. The recurrence rate after GTR was lower after EEA compared with TCA (OR 0.33, P = .0027).

CONCLUSION:
EEA for appropriately selected TSM may lead to better visual outcomes and decreased recurrence rates after GTR, but cerebrospinal fluid leak rates are high, and longer follow-up is needed. Tumors were smaller in the EEA group, and follow-up was shorter, reflecting selection, and observation bias. Nevertheless, EEA may be superior to TCA for appropriately selected TSM.
Extreme Lateral Approach to the Craniocervical Junction, Operative Technique and Approach Essentials: 2-Dimensional Operative Video
Authors: Ber Roee , Kay-Rivest Emily , Sen Chandra 
Published: June 2023

INDICATIONS: CORRIDOR AND LIMITS OF EXPOSURE:
The extreme lateral approach is useful for both extradural and intradural anterior and anterolateral lesions at the lower clivus down to the level of C2.

ANATOMIC ESSENTIALS: NEED FOR PREOPERATIVE PLANNING AND ASSESSMENT:
The patient is evaluated with MRI, computed tomography (CT), and an angiogram. Special attention is given to vascular (vertebral artery course, dominance, tumor feeders) and bony (occipital condyle, jugular tubercle, foramen magnum and extent of bony involvement) anatomy.

ESSENTIALS STEPS OF THE PROCEDURE:
The patient is positioned lateral with the head flexed and tilted down without axial rotation. A hockey-stick incision is performed, and the myocutaneous flap is raised. A retrocondylar craniectomy is performed. The extradural vertebral artery is exposed for proximal control. A C1 hemilaminectomy is performed. Cephalad/caudal exposure and drilling of the occipital condyle are determined per case. The dura is opened, and the vertebral artery is released at the dural entry point to facilitate the tumor removal. The tumor is debulked and delivered inferoventrally away from the neuroaxis and cranial nerves. After removing the tumor, the dura is closed using an allograft.
The patients consented to the procedure and to the publication of their images.

PITFALLS/AVOIDANCE OF COMPLICATIONS:
• Cranial nerve deficits
• Craniocervical instability
• Postoperative hydrocephalus
• Postoperative pseudomeningocele

VARIANTS AND INDICATIONS FOR THEIR USE:
A transmastoid extension of the craniectomy allows access further rostrally in the clivus. For C1-2 chordomas, the approach is extended inferiorly, and the vertebral artery is mobilized out of the C1-2 transverse foramina. For tumors involving the joints, an occipitocervical stabilization is required.
Images in video reused with permission as follows: image at 00:16 from Revuelta Barbero et al, Endoscopic endonasal transclival-medial condylectomy approach for resection of a foramen magnum meningioma: 2-dimensional operative video, Oper Neurosurg, 16(2), 2018, by permission from the Congress of Neurological Surgery; images at 00:30, and top image at 00:52 reused from Wen et al, Microsurgical anatomy of the transcondylar, supracondylar, and paracondylar extensions of the far-lateral approach, J Neurosurg, 87(4), 1997, with permission from JNSPG; bottom images at 00:52 from Muthukumar et al, A morphometric analysis of the foramen magnum region as it relates to the transcondylar approach, Acta Neurochir, 147(8), 2005, by permission from Springer Nature.
Single-cell proteomics: quantifying post-transcriptional regulation during development with mass-spectrometry
Authors: Slavov Nikolai 
Published: June 2023

ABSTRACT
Many developmental processes are regulated post-transcriptionally. Such post-transcriptional regulatory mechanisms can now be analyzed by robust single-cell mass spectrometry methods that allow accurate quantification of proteins and their modification in single cells. These methods can enable quantitative exploration of protein synthesis and degradation mechanisms that contribute to developmental cell fate specification. Furthermore, they may support functional analysis of protein conformations and activities in single cells, and thus link protein functions to developmental processes. This Spotlight provides an accessible introduction to single-cell mass spectrometry methods and suggests initial biological questions that are ripe for investigation.
Modeling development using hydrogels
Authors: Xu Karen L. , Mauck Robert L. , Burdick Jason A. 
Published: June 2023

ABSTRACT
The development of multicellular complex organisms relies on coordinated signaling from the microenvironment, including both biochemical and mechanical interactions. To better understand developmental biology, increasingly sophisticated in vitro systems are needed to mimic these complex extracellular features. In this Primer, we explore how engineered hydrogels can serve as in vitro culture platforms to present such signals in a controlled manner and include examples of how they have been used to advance our understanding of developmental biology.
A Synthetic Strategy for Multi-Functionalized Phospharhodamines via Catalytic P-Arylation
Authors: Asada Yuichi , Taki Masayasu , Yamaguchi Shigehiro 
Published: June 2023

Abstract
The visualization of complex biological events in living cells is highly reliant on the development of sophisticated fluorescent probes with multiple functions. Herein, we report a general synthetic strategy for the synthesis of near-infrared-emissive phospharhodamines (PORs) bearing various types of aryl groups on their bridging phosphorus atoms. This versatile approach utilizes a Ni-catalyzed P-arylation that allows for the design of POR-based multi-functionalized fluorescent probes. We found that PORs substituted with an aminophenyl group exhibited pH-responsive fluorescence intensity, where photoinduced electron transfer occurs to quench the fluorescence. The combination of our new approach with conventional modification at the 9-position of the xanthene skeleton facilitates the development of multi-functionalized fluorescent probes. As one demonstration of this method, we synthesized a fluorescence probe that can selectively visualize the lysosomes that result from endosomal maturation.
Assessing use of vancomycin powder in craniotomy: randomized controlled trial (AVIC)
Authors: Belkhair Sirajeddin , khan Muhammad Mohsin , Baregzai Younis , Walizada Khalida , Eid Ahmed , Taha Ahmed , Safi Saleh , Mohammad Amr , llleyyan Abdullah , Ben Zabih Tarek , Raza Ali , khan Adnan , Hammadi Firas , Jarir Raed , Ayyad Ali , Alrabayah Talal 
Published: June 2023

Background: Surgical-site infections (SSIs) can lead to greater postoperative morbidity, mortality, and health care costs. Despite current prophylactic measures, rates of SSIs have been reported in up to 5% of patients post craniotomy. Intrawound vancomycin powder has been studied extensively in spinal fusion surgeries and been found to reduce rates of surgical site infections (SSIs) significantly. Despite its success in spinal surgeries, topical vancomycin has not been extensively studied with respect to cranial neurosurgery. Methods: Our study is Prospective Randomized clinical trial. Patients will be divided in this Trial into two groups, first group (intervention arm) they will receive the drug (vancomycin) in the wound before the closure of the skin at the end of the surgical procedure. The second group (control arm) they will not receive the drug, otherwise both groups they will receive identical measure to decrease the postoperative SSI. The primary outcome variable will be SSI rate factored by cohort. Secondary outcome will be to monitor the safety and any complication related to the use of vancomycin . SSI found to be around 0.49% when vancomycin was used, while SSI in standard care found to be 5%, to get power of study 80% and level of significance 5%. Sample size will be 250 in each group using sample size calculator. Discussion: This study is designed to evaluate the efficacy of vancomycin compared to standard method in neuro-surgical cases undergoing craniotomy . Additionally, safety of vancomycin will be assessed in these patients.
Pisarze asyryjscy związani z miastem Nisibis: ich życie i dzieła
Authors: Abdalla Michael 
Published: June 2023

W opracowaniu przedstawiono w zarysie miasto Nisibis i jego szkołę oraz związanych z nim pisarzy i ich dorobek. Mało które miasto bliskowschodnie odegrało tak dużą rolę w chrześcijaństwie w ciągu IV-VII w. jak Nisibis. W nim urodzili się i działali św. Jakub (†338), aktywny uczestnik soboru nicejskiego (325) i św. Efrem (†373), pierwszy i powszechnie uznany poeta chrześcijański. Prężnie działająca w mieście szkoła wyższa wykształciła dziesiątków myślicieli i ludzi pióra, w tym 10 patriarchów i 60 biskupów i już w V wieku Nisibis nazwano ܐܡܐ ܕܝܘܠܦܢ̈ܐ Emma d-Yulpāne (Alma Mater).
Zestawienie pisarzy sporządzono chronologicznie, ograniczając się tylko do Kościoła Asyryjskiego. Napisane przez nich dzieła pozwolą wyrobić pogląd o tematyce, którą się zajmowali, będącej odbiciem warunków życia duchowego nie tylko ludzi Kościoła, lecz także społeczności wiejskich i wiejskich. Przekonamy się też, jaką rolę odegrali w dyplomacji i w dialogu międzykulturowym jako pomost między Bizancjum i Persją.
Preoperative investigations in thyroglossal duct cyst surgery: a 9-year experience and proposed practice guide
Authors: Mettias B , Cole S , Valsamakis T 
Published: June 2023

Introduction Thyroglossal duct cysts (TGDC) are the commonest midline developmental cyst and have a bimodal age distribution. They usually develop in an infrahyoid position. A National survey of TGDC practice among otolaryngologists in 2012 recommended preoperative investigation with ultrasound, with or without blood tests. Methods Retrospective analysis of preoperative investigation for clinically diagnosed TGDC surgeries between 2012 and 2020 in a single tertiary centre was undertaken. This was collated alongside postoperative outcomes (histology, recurrence, hypothyroidism). Comparison was made with the 2012 national survey. Results Ninety-five cases of thyroglossal duct surgery in children and adults were studied. Demographic data were comparable to the literature. Ultrasonography was the most commonly used preoperative investigation. Histology of 71% of excised cysts confirmed TGDC with another 8% reported as development cysts. The excision of the cyst with a cuff of strap muscles and middle portion of hyoid bone had the lowest recurrence rate, which was 4% overall in this study. There were no cases of ectopic thyroid tissue or postoperative hypothyroidism. Discussion and conclusion Nearly a decade of thyroglossal duct cyst excisions in a large-volume unit gave detail on actual preoperative practice and outcomes. Practice was found to be consistent overall with the 2012 recommendations although not standardised across all cases. From this experience, and a literature review, a visual guide on preoperative investigations for different age groups in the form of a flow chart is proposed to reduce risk of complications while avoiding unnecessary investigations.
Facilitators and barriers of routine psychosocial distress assessment within a stepped and collaborative care model in a Swiss hospital setting
Authors: Aebi Nicola Julia , Baenteli Iris , Fink Günther , Meinlschmidt Gunther , Schaefert Rainer , Schwenkglenks Matthias , Studer Anja , Trost Sarah , Tschudin Sibil , Wyss Kaspar ,   the SomPsyNet Consortium
Published: June 2023

Background
Stepped and Collaborative Care Models (SCCMs) have shown potential for improving mental health care. Most SCCMs have been used in primary care settings. At the core of such models are initial psychosocial distress assessments commonly in form of patient screening. We aimed to assess the feasibility of such assessments in a general hospital setting in Switzerland.

Methods
We conducted and analyzed eighteen semi-structured interviews with nurses and physicians involved in a recent introduction of a SCCM model in a hospital setting, as part of the SomPsyNet project in Basel-Stadt. Following an implementation research approach, we used the Tailored Implementation for Chronic Diseases (TICD) framework for analysis. The TICD distinguishes seven domains: guideline factors, individual healthcare professional factors, patient factors, professional interactions, incentives and resources, capacity for organizational change, and social, political, and legal factors. Domains were split into themes and subthemes, which were used for line-by-line coding.

Results
Nurses and physicians reported factors belonging to all seven TICD domains. An appropriate integration of the psychosocial distress assessment into preexisting hospital processes and information technology systems was the most important facilitator. Subjectivity of the assessment, lack of awareness about the assessment, and time constraints, particularly among physicians, were factors undermining and limiting the implementation of the psychosocial distress assessment.

Conclusions
Awareness raising through regular training of new employees, feedback on performance and patient benefits, and working with champions and opinion leaders can likely support a successful implementation of routine psychosocial distress assessments. Additionally, aligning psychosocial distress assessments with workflows is essential to assure the sustainability of the procedure in a working context with commonly limited time.