bluuebunny/crossref_metadata_2025_split huggingface dataset:
High-throughput sequencing approach for the identification of lncRNA biomarkers in hepatocellular carcinoma and revealing the effect of ZFAS1/miR-150-5p on hepatocellular carcinoma progression
Authors: Zhu Peng , Pei Yongyan , Yu Jian , Ding Wenbin , Yang Yun , Liu Fuchen , Liu Lei , Huang Jian , Yuan Shengxian , Wang Zongyan , Gu Fangming , Pan Zeya , Chen Jinzhong , Qiu Jinrong , Liu Huiying 
Published: February 2023

Aims
To screen abnormal lncRNAs and diagnostic biomarkers in the progression of hepatocellular carcinoma through high-throughput sequencing and explore the underlying mechanisms of abnormal lncRNAs in the progression of hepatocellular carcinoma.

Methods
The transcriptome sequencing was used to analyze the RNA expression profile and identify differentially expressed RNAs. Hub lncRNAs were screened by combining (WGCNA, ceRNA regulatory network, PPI, GO and KEGG analyses, Kaplan-Meier curve analysis, Cox analysis, risk model construction and qPCR). Thereafter, the correlation between the expression of hub lncRNAs and tumor clinicopathological parameters was analyzed, and the hub lncRNAs were analyzed by GSEA. Finally, the effects of hub RNAs on the proliferation, migration and invasion of HepG2 cells were investigated in vitro.

Results
Compared with the control group, a total of 610 lncRNAs, 2,593 mRNAs and 26 miRNAs were screened in patients with hepatocellular carcinoma. Through miRNA target prediction and WGCNA, a ceRNA was constructed, comprising 324 nodes and 621 edges. Enrichment analysis showed that mRNAs in ceRNA were involved mainly in cancer development progression. Then, the ZFAS1/miR-150-5p interaction pair was screened out by Kaplan Meier curve analysis, Cox analysis and qPCR analysis. Its expression was related to tumor stage, TNM stage and patient age. ROC curve analysis showed that it has a good predictive value for the risk of hepatocellular carcinoma. GSEA showed that ZFAS1 was also enriched in the regulation of immune response, cell differentiation and proliferation. Loss-of-function experiments revealed that ZFAS1 inhibition could remarkably suppress HepG2 cell proliferation, migration and invasion in vitro. Bioinformatic analysis and luciferase reporter assays revealed that ZFAS1 directly interacted with miR-150-5p. Rescue experiments showed that a miR-150-5p inhibitor reversed the cell proliferation, migration and invasion functions of ZFAS1 knockdown in vitro.

Conclusion
ZFAS1 is associated with the malignant status and prognosis of patients with hepatocellular carcinoma, and the ZFAS1/miR-150-5p axis is involved in hepatocellular carcinoma progression.
Synbiotic Yogurt Fortified with Bawang Dayak (Eleutherine palmifolia) Extract
Authors: Andriani R. D. , Sawitri M. E. , Widayanti V. T. , Manab A. , Rahayu P. P. , Fadilah R. N. 
Published: February 2023

Aims: The purpose of this research was to study the quality of yogurt symbiotic fortified with Bawang Dayak (Eleutherine palmifolia) extract.
Study Design: This research used Completely Randomized Design (CRD) experimental method and consist of 4 treatments.
Place and Duration of Study: Sample: Animal Product Technology Laboratory Faculty of Animal Science Universitas Brawijaya, Food Quality and Safety Testing Laboratory Department of Agricultural Product Technology, Faculty of Agricultural Technology Universitas Brawijaya, between June 2022 – December 2022.
Methodology: The material used are skim milk, fresh milk, yogurt starter bacteria (Lactobacillus bulgaricus, Streptococcus thermophilus, Lactobacillus acidophillus), and bawang dayak extract. Bawang Dayak were extracted using Microwave-Asisted Extraction (MAE) with temperature 60-700C. This research which consist of 4 treatments and 3 replications with various concentration of Bawang Dayak extract of T0 (0%), T1 (1%), T2 (4%), and T3 (6%), respectively. The samples of synbiotic yogurt with fortification of Bawang Dayak extract were analyzed for their total lactic acid bacteria, antioxidant activity, viscosity and color analysis.
Results: Fortification of Bawang dayak extract in synbiotic yogurt results in highly significant different effects on the quality parameters of antioxidant and color (lightness, redness and yellowness) (P<0.01), but had no significant effect (P>0.05) on the total quality of lactic acid bacteria. Fortification of 6% Bawang dayak extract (T3) in yogurt produced the best quality in terms of antioxidant content and color (lightness, redness, and yellowness).
Conclusion: The best treatment of synbiotic yogurt was achieved in T3 with the 6% of Bawang Dayak extract.
Comparative Assessment of Farmer’s Brinjal Variety in Different Agro-Climatic Conditions
Authors: Choudhary Hardev , Dave Parthkumar P. , Parvez Noushad , Chodvadiya Mahesh B. , Ritaparna Prajana , Khuman Laishram Yelhounganba , Ipe Chriss , Sahu Leelaram 
Published: February 2023

In order to evaluate the performance and suitability of the long brinjal variety ‘Niranjan Bhata’ developed by an innovative farmer of Chhattisgarh in different agro-climatic conditions of the country, the comparative field experiment conducted under Gandhinagar, Gujarat condition was accommodated in a randomized complete block design comprising five replications of four treatments, i.e. Niranjan Bhata, Pusa Shamla as National Check (NC) and two Local Check (LC) during June - March 2016. The plant height of Niranjan Bhata (115.50 cm) was recorded at par with LC-1 (118.33 cm). However, the maximum fruit length (45.80 cm) was recorded in Niranjan Bhata (45.80 cm). The significant highest fruit yield was produced by Niranjan Bhata (31.40 t/ha).
Farmer managed trials (FMTs) were also carried out in five states between 2017-18 and 2020-21 at 68 locations (loc.) during the Kharif season. The test variety ‘Niranjan Bhata’ reported superior performance in Chhattisgarh with the highest plant height (161.17 cm), fruit length (59.67 cm), and marketable fruit yield (9.81 kg/plant). The variety also outperformed with similar characters in Gujarat and Maharashtra states over checks. As per the growers' feedback, the variety was found to be high-yielding. The long fruits containing lesser seeds and soft pulp having good market preference too. Thus the evaluation of variety with the farmers’ participation was found most suitable approach which assured the varietal preference and its suitability for large-scale dissemination.
Assessment of Available Water and Crop Water Requirements of Major Crops in Nagarjuna Sagar Right Canal Command Area, Andhra Pradesh, India
Authors: Rao D. Sai Gangadhara , Krishna Ch. Murali , Kumar H. V. Hema , Devi B. Sarojini , Edukondalu L. , Rao V. Srinivasa 
Published: February 2023

The mean water releases at head section of 11 years of Nagarjuna Sagar Jawahar right main Canal, Andhra Pradesh, India is found to be 128.13 TMC (3,627.71 MCM). The mean surface water availability was estimated as 2,229 MCM. For the efficient utilization of groundwater, the quality plays a major role in terms of selection of crop variety for sustainable agriculture. The ground water quantity of the command area available was estimated 1385 MCM and the mean annual conjunctive use of command area was 3,614 MCM (128 TMC).
The crop water requirement for paddy, cotton, chilies, millet and pulses were estimated in the command area by using CROPWAT model. The crop water requirement for paddy in NSRC command area of Guntur district for black soils was 848.2 mm. The irrigation water requirement was 638.9 mm and for red soils, crop water requirement for paddy and irrigation water requirements were 848.2 mm and 624.5 mm respectively. Similarly, computed for all the crops and overall crop water demand for both (Guntur and Prakasam) districts of NSRC command area for different soils and different crops per annum was 4,456 MCM without considering cattle and domestic water use. Based on the research, it was concluded that the available water is not sufficient and it is suggested to choose alternative crops for bringing entire area into cultivation.
Role of Magnetic Resonance Imaging and Magnetic Resonance Spectroscopy in Evaluation of Different Types of Dementia
Authors: Esawy Abeer Salah Abd El Razek El , Younis Rasha Lottfy , Din ELSayed Aly Mohamed Tag EL , Dawoud Mahmoud Abd El Aziz 
Published: February 2023

Aims: The aim of this work is to study the role of magnetic resonance imaging and magnetic resonance spectroscopy in evaluation of dementia patients.
Patients and Methods: This study conducted on fifty patients referred to radio diagnosis and medical imaging department from Neuropsychiatry department at Tanta University Hospital with clinical diagnosis of different types of dementia.
Results: The grade of medial temporal lobe atrophy was higher in AD than in the other two types of dementia. Vascular lesions that fulfill these criteria were present in all patients diagnosed as VaD and their Fazekas score was 3, while patients diagnosed as AD and PDD didn’t have vascular lesions that fulfill NINDS-AIREN criteria and Fazekas scoring of these patients did not exceed 2. Decreased NAA/Cr ratio in the posterior cingulate gyrus was less valuable in diagnosing AD when compared with elevated mI/Cr. Decreased NAA/Cr ratio in the centrum semiovale, Decreased NAA/Cr ratio in the occipital lobe.
Conclusion: MRS is complementary to MRI revealing variable underlying metabolic abnormalities in different dementia subtypes even in cases with apparently normal conventional MRI series. Elevated mI/Cr ratio in the posterior cingulate gyrus is useful indicator when assessing Alzheimer’s dementia and also differentiating it from Vascular dementia and Parkinson’s disease dementia. Decrease in NAA/Cr ratio in the centrum semiovale is useful indicator when assessing suspected vascular dementia. Decrease in NAA/Cr ratio in the occipital lobe is useful indicator when assessing suspected Parkinson’s disease dementia.
A Comparative Model Analysis of Predictors of Community Pharmacists’ Collaborative Practices in Two Southwestern States in Nigeria
Authors: Oamen Theophilus Ehidiamen 
Published: February 2023

Background: In community pharmacy practice, pharmacists are expected to engage in collaborative activities to deliver quality service to clients. However, due to the hybrid nature (social and commercial dimensions) of the practice, certain subjective behavioral norms-competitive and information-sharing behavior may have potential influence on the collaborative behaviors of community pharmacists
Objectives: Based on the theory of planned behavior framework, the study tests the influence of subjective norms-competitive behavior and information-sharing practices on the collaborative behavior of community pharmacists; and, investigated possible differences in the perception of respondents in two southwestern states in Nigeria
Methods: A cross-sectional quantitative study with randomly administered questionnaires to 421 community pharmacists from two purposively selected states- Lagos (230) and Ogun (191) in southwest Nigeria. A structural equation model was developed using Analysis of Moment Structures software (AMOS). Group differences were evaluated using the chi-square difference test.
Results: Competitive behavior and information-sharing behavior are substantial predictors of collaborative behaviors among community pharmacists (R2=0.97, p<0.001). Multigroup analysis revealed differences in perception between respondents in the densely populated and less densely populated settings. Model comparisons revealed insignificant group differences.
Conclusion: The study confirmed that information-sharing and competitive behavior have a positive and significant influence on collaborative behavior. The findings of the study imply that no link exists between the geographical placement of community pharmacists and differences in their practice behavior. Professional associations and regulatory bodies should provide training and engage in participatory workshops to strengthen the right collaborative attitudes among community pharmacists. The study provides a theoretical framework for further studies in inter- and intra-professional relations among healthcare professionals.
Experimental Study of Self-Compacting Concrete Using Plantain Leaf Ash and Polycarboxylate Ether Solution as Superplasticizer
Authors: Tochukwu Mmeka Paschal , Emeka Ugwu Stephen , Chisomnazu Iwenofu Alvin 
Published: February 2023

Concrete is a binding material, made up of constituent of cement, fine aggregate, coarse aggregate and water. Self-Compacting Concrete is an innovative concrete that does not require vibration for placing and compaction. It is able to flow under its own weight, completely filling formwork and achieving full compaction, even in the presence of congested reinforcement. The study also intended to quantify the amount of plantain leaf ash to be added to the concrete according to the value of concrete properties measured. For such purpose, the fresh concrete must possess intense fluidity and great cohesiveness. Plantain leaf ash is waste and is causing threat to environment in terms of odour and to reduce this problem of this material the project has been undertaken so that it can be used for construction purpose. The following points were attempted for the resdearch;
i. To replace cement by different percentage of Plantain leaf ash,
ii. To prepare concrete by replacing the cement by Plantain leaf ash,
iii. To compare the fresh properties of self-compacting concrete containing varying amounts of plantain leaf ash with that containing commercially available superplasticizer,
iv. To study the compressive strength of normal conventional concrete and self-compacting concrete.
Analysis of Financial Statement Preparation, Fiscal Reconciliation and Human Resources Competency on Corporate Income Tax of Sriwijaya Argo Sakti Company, Indonesia
Authors: Yunita Hesti 
Published: February 2023

Aims: This study aims to find out, analyze, and how the competence of human resources in the internal PT SAS and fiscal correction and analyze whether the competence of internal human resources is an obstacle or supporting factor in preparing financial statements.
Study Design: The research design used in this study is a qualitative descriptive research design using the post-positivism paradigm with the triangulation method, which is an experimental approach using various methods, data sources, researchers, and theories.
Place and Duration of Study: The researcher set an analysis unit on the Corporate Income Tax (PPh) Fiscal Correction and reviewed PT SAS's 2020 financial statements with Human Resources (HR) as an influential factor. Sampling determination in this study used a purposive sampling technique.
Methodology: The sampling determination in this study used purposive sampling techniques with the criteria of PT SAS Tax Managers, Tax Consultants, Accounting Staff, and Tax Staff. The data collection used in this study can be grouped into two categories, namely interactive and non-interactive methods.
Results: Based on the study's results, a fiscal correction in corporate income tax was caused by HR errors that did not record accounts receivable. Then, there is the fiscal correction. From the aspect of knowledge (knowledge), attitude (attitude) and aspects of skills (skills), there are still problems that still need to be solved.
Conclusion: The company must be more thorough in making corrections to the costs that the company has incurred according to the rules. Companies should more often provide education and training in the field of accounting and taxation to employees. The company also advises the directorate general of taxes to review related CSR Costs.
Multinational Analysis of Estimated Health Care Costs Related to Extended-Interval Fixed Dosing of Checkpoint Inhibitors
Authors: Goldstein Daniel A. , Ginsberg Gary M. , Hofnung-Gabbay Dorit , De Abreu Lourenco Richard , Loong Herbert H. , Goh Boon Cher , Chan Kelvin K. W. , Di Maio Massimo , Perrone Francesco , Hall Peter S. , Zer Alona , Rosenbaum Eli 
Published: February 2023

ImportanceNew dosing options for immune checkpoint inhibitors have recently been approved by the US Food and Drug Administration (FDA), including fixed dosing with extended intervals. Although the dose intensity appears the same, there is expected to be some waste with extended-interval dosing, as some drug remains in the bloodstream once a decision to stop treatment is made. The economic impact of extended-interval fixed dosing is unknown compared with standard-interval fixed dosing.ObjectiveTo analyze the potential health care costs of using extended-interval fixed dosing instead of standard-interval fixed dosing.Design, Setting, and ParticipantsThis economic evaluation used a pharmacoeconomic model to simulate 2 cohorts of patients with platinum-resistant metastatic urothelial cancer receiving pembrolizumab as second-line therapy at different dosing intervals using 2020 pricing data. Data were analyzed from 2020 to 2022.ExposuresThe simulated patients received FDA-approved regimens of either 200 mg every 3 weeks or 400 mg every 6 weeks.Main Outcomes and MeasuresThe progression-free survival curve from the KEYNOTE-045 trial was used to estimate treatment duration. Drug, imaging, and administration costs were included in analyses. Sensitivity analyses were performed to assess how different imaging frequencies would affect the model results. The potential overall costs of using the 2 different dosing strategies were assessed. The base case was set in the US, while sensitivity analyses were set in several other countries.ResultsIn the base case analysis, dosing every 6 weeks instead of every 3 weeks resulted in an estimated 8.9% increase in pembrolizumab costs for the health care payer. Accounting for a decrease in infusion costs would result in an estimated net additional cost of $7483 per patient in the US (7.9% cost increase). In the US, this would amount to an increase of approximately $28 million annually for health care payers. Similar percentages in cost estimate increases were found for health care payers around the world, such as in Israel, where the net additional cost would be $5491 per patient.Conclusions and RelevanceThis economic evaluation assessed and quantified the potential increased costs related to extended-interval fixed dosing of pembrolizumab. The model method could be applied to other diseases and other drugs for which there has been a movement toward extended-interval dosing. Results may differ in other diseases owing to differing disease courses and patient profiles.
Detection of the Omicron BA.1 Variant of SARS-CoV-2 in Wastewater From a Las Vegas Tourist Area
Authors: Vo Van , Tillett Richard L. , Papp Katerina , Chang Ching-Lan , Harrington Anthony , Moshi Michael , Oh Edwin C. , Gerrity Daniel 
Published: February 2023

ImportanceInterpretation of wastewater surveillance data is potentially confounded in communities with mobile populations, so it is important to account for this issue when conducting wastewater-based epidemiology (WBE).ObjectivesTo leverage spatial and temporal differences in wastewater whole-genome sequencing (WGS) data to quantify relative SARS-CoV-2 contributions from visitors to southern Nevada.Design, Setting, and ParticipantsThis cross-sectional wastewater surveillance study was performed during the COVID-19 pandemic (March 2020 to February 2022) and included weekly influent wastewater samples that were analyzed by reverse transcription–quantitative polymerase chain reaction to quantify SARS-CoV-2 RNA and WGS for identification of variants of concern. This study was conducted in the Las Vegas, Nevada, metropolitan area, which is a semi-urban area with approximately 2.3 million residents and nearly 1 million weekly visitors. Samples were collected from 7 wastewater treatment plant (WWTP) locations that collectively serve the vast majority of southern Nevada (excluding the small number of septic systems) and 1 manhole serving the southern portion of the Las Vegas Strip. With Las Vegas tourism returning to prepandemic levels in 2021, it was hypothesized that visitors were contributing a disproportionate fraction of SARS-CoV-2 RNA to the largest WWTP in southern Nevada, potentially confounding efforts to estimate COVID-19 incidence in the local community through WBE.Main Outcomes and MeasuresRelative SARS-CoV-2 load and variants from visitors vs the local population.ResultsThe Omicron BA.1 VOC was detected in the Las Vegas Strip manhole approximately 1 week before its detection at the WWTP locations (December 13, 2021) and by clinical testing (December 14, 2021). On December 13, Omicron-specific mutations represented a mean (SD) of 48.0% (4.2%) of all genomes from the Las Vegas Strip manhole and 4.1% (1.4%) of all genomes at facilities 2 and 3; by December 20, Omicron-specific mutations represented means (SD) of 82.0% (3.0%) of all genomes at the Las Vegas Strip manhole and 48.0% (2.8%) of all genomes at facilities 2 and 3, respectively. During this time, it was estimated that visitors contributed more than 60% of the SARS-CoV-2 load to the sewershed serving the Las Vegas Strip and that Omicron prevalence among visitors was 40% to 60% on December 13 and 80% to 100% on December 20th.Conclusions and RelevanceWastewater surveillance is a valuable complement to clinical tools and can provide time-sensitive data for decision-makers and policy makers. This study represents a novel approach for quantifying the confounding effects of mobile populations on wastewater surveillance data, thereby allowing for modification of an existing WBE framework for estimating COVID-19 incidence in southern Nevada.
Estimates and Projections of the Global Economic Cost of 29 Cancers in 204 Countries and Territories From 2020 to 2050
Authors: Chen Simiao , Cao Zhong , Prettner Klaus , Kuhn Michael , Yang Juntao , Jiao Lirui , Wang Zhuoran , Li Weimin , Geldsetzer Pascal , Bärnighausen Till , Bloom David E. , Wang Chen 
Published: February 2023

ImportanceCancers are a leading cause of mortality, accounting for nearly 10 million annual deaths worldwide, or 1 in 6 deaths. Cancers also negatively affect countries’ economic growth. However, the global economic cost of cancers and its worldwide distribution have yet to be studied.ObjectiveTo estimate and project the economic cost of 29 cancers in 204 countries and territories.Design, Setting, and ParticipantsA decision analytical model that incorporates economic feedback in assessing health outcomes associated with the labor force and investment. A macroeconomic model was used to account for (1) the association of cancer-related mortality and morbidity with labor supply; (2) age-sex-specific differences in education, experience, and labor market participation of those who are affected by cancers; and (3) the diversion of cancer treatment expenses from savings and investments. Data were collected on April 25, 2022.Main Outcomes and MeasuresEconomic cost of 29 cancers across countries and territories. Costs are presented in international dollars at constant 2017 prices.ResultsThe estimated global economic cost of cancers from 2020 to 2050 is $25.2 trillion in international dollars (at constant 2017 prices), equivalent to an annual tax of 0.55% on global gross domestic product. The 5 cancers with the highest economic costs are tracheal, bronchus, and lung cancer (15.4%); colon and rectum cancer (10.9%); breast cancer (7.7%); liver cancer (6.5%); and leukemia (6.3%). China and the US face the largest economic costs of cancers in absolute terms, accounting for 24.1% and 20.8% of the total global burden, respectively. Although 75.1% of cancer deaths occur in low- and middle-income countries, their share of the economic cost of cancers is lower at 49.5%. The relative contribution of treatment costs to the total economic cost of cancers is greater in high-income countries than in low-income countries.Conclusions and RelevanceIn this decision analytical modeling study, the macroeconomic cost of cancers was found to be substantial and distributed heterogeneously across cancer types, countries, and world regions. The findings suggest that global efforts to curb the ongoing burden of cancers are warranted.