bluuebunny/crossref_metadata_2025_split huggingface dataset:
Trends in clinical characteristics and outcomes of all critically ill COVID-19 adult patients hospitalized in France between March 2020 and June 2021: a national database study
Authors: Naouri Diane , Vuagnat Albert , Beduneau Gaëtan , Dres Martin , Pham Tai , Mercat Alain , Combes Alain , Demoule Alexandre , Kimmoun Antoine , Schmidt Matthieu , Jamme Matthieu 
Published: January 2023

Abstract
Introduction
Studies regarding coronavirus disease 2019 (COVID-19) were mainly performed in the initial wave, but some small-scale data points to prognostic differences for patients in successive waves. We therefore aimed to study the impact of time on prognosis of ICU-admitted COVID-19 patients.

Method
We performed a national retrospective cohort study, including all adult patients hospitalized in French ICUs from March 1, 2020 to June 30, 2021, and identified three surge periods. Primary and secondary outcomes were in-hospital mortality and need for invasive mechanical ventilation, respectively.

Results
105,979 critically ill ICU-admitted COVID-19 patients were allocated to the relevant three surge periods. In-hospital mortality for surges 1, 2, and 3 was, respectively, 24%, 27%, and 24%. Invasive mechanical ventilation was the highest level of respiratory support for 42%, 32%, and 31% (p < 0.001) over the whole period, with a decline in the use of vasopressors over time. Adjusted for age, sex, comorbidities, and modified Simplified Acute Physiology Score II at ICU admission, time period was associated with less invasive mechanical ventilation and a high risk of in-hospital death. Vaccination against COVID-19 was associated with a lower likelihood of invasive mechanical ventilation (adjusted sub-hazard ratio [aSHR] = 0.64 [0.53–0.76]) and intra-hospital death (aSHR = 0.80, [0.68–0.95]).

Conclusion
In this large database of ICU patients admitted for COVID-19, we observed a decline in invasive mechanical ventilation, vasopressors, and RRT use over time but a high risk of in-hospital death. Vaccination was identified as protective against the risk of invasive mechanical ventilation and in-hospital death.
Hand pose estimation based on improved NSRM network
Authors: Yang Shiqiang , He Duo , Li Qi , Wang Jinhua , Li Dexin 
Published: January 2023

AbstractHand pose estimation is the basis of dynamic gesture recognition. In vision-based hand pose estimation, the performance of hand pose estimation is affected due to the high flexibility of hand joints, local similarity and severe occlusion among hand joints. In this paper, the structural relations between hand joints are established, and the improved nonparametric structure regularization machine (NSRM) is used to achieve more accurate estimation of hand pose. Based on the NSRM network, the backbone network is replaced by the new high-resolution net proposed in this paper to improve the network performance, and then the number of parameters is decreased by reducing the input and output channels of some convolutional layers. The experiment of hand pose estimation is carried out by using public dataset, the experimental results show that the improved NSRM network has higher accuracy and faster inference speed for hand pose estimation.
Caspase-8 contributes to an immuno-hot microenvironment by promoting phagocytosis via an ecto-calreticulin-dependent mechanism
Authors: Gong Zhihua , Jia Qingzhu , Guo Jinming , Li Chongyi , Xu Shouxia , Jin Zheng , Chu Han , Wan Yisong Y. , Zhu Bo , Zhou Yi 
Published: January 2023

Abstract
Background
Caspase-8 (Casp8) acts as an initiator in cell apoptosis signaling. However, the role of Casp8 in tuning the tumor immune microenvironment remains controversial due to the complicated crosstalk between immune-tolerogenic apoptotic cell death and immunogenic cell death cascades.

Methods
The Cancer Genome Atlas (TCGA) and publicly accessible immune checkpoint blockade (ICB)-treated cohorts were used to investigate the clinical relevance of Casp8. A tumor-bearing mouse model was used to characterize changes in the tumor microenvironment and to explore the efficacy of ICB treatment under Casp8 knockout conditions.

Results
By exploring TCGA datasets, we showed that the expression level of Casp8 was associated with an immuno-hot microenvironment across various solid tumor types. Casp8 deficiency leads to decreased CD8+ T cell infiltration and resistance to anti-PD-L1 therapy in a mouse model. Mechanistically, Casp8 deficiency or pharmacological disruption results in impaired ecto-calreticulin transition in tumor cells, which in turn hampers antigen presentation in draining lymph nodes. Furthermore, radiotherapy restored sensitivity to anti-PD-L1 treatment via elevated calreticulin surface expression.

Conclusions
Our data revealed a causative role of Casp8 in modulating the immunogenicity of tumor cells and responsiveness to ICB immunotherapies and proposed radiotherapy as a salvage approach to overcome Casp8 deficiency-mediated ICB resistance.
Spatial ecology of translocated American Eel (Anguilla rostrata) in a large freshwater lake
Authors: Stahl Aliénor , Larocque Sarah M. , Gardner-Costa Jesse , Mathers Alastair , Pratt Thomas C. , Schlueter Scott , Midwood Jonathan D. 
Published: January 2023

AbstractAmerican Eel (Anguilla rostrata) undertake extensive migrations from their rearing grounds to spawn in the Sargasso Sea, and historically the upper St. Lawrence River and Lake Ontario provided an important source for large, fecund female eel. Following declines in the Lake Ontario population, glass eel were translocated from eastern Canada from 2006 to 2010. From 2016 to 2018, large, presumably translocated yellow eel (N = 230) with the potential to begin maturing and out-migrating within their year of capture were collected in spring and fall and tagged with acoustic transmitters. Eel were released into eastern Lake Ontario and tracked to better understand their movement patterns prior to and during migration, and the timing of migration. Most eels successfully migrated out of Lake Ontario (64%). Timing of migration was consistent regardless of year or tagging season and primarily occurred in late summer or fall, with cooling water temperatures and decreasing sky illumination associated with initiation for fall tagged eel. Eels were mostly detected in eastern Lake Ontario and those in western Lake Ontario were mostly detected in shallow waters (< 20 m) close to shore. Eels were detected on fewer receivers in the winter, suggesting reduced movements during this season. Finally, larger individuals spent less time in the system, particularly when tagged in the fall. These findings confirm that translocated eels can migrate out of Lake Ontario; however, the weeks when migration occurred were more aligned with timing in their natal range (i.e., eastern Canada) than with naturally recruited eels from Lake Ontario. This temporal mismatch requires further consideration, since it may influence arrival times of translocated eel to the spawning grounds and their recruitment potential. These results can be used to inform future assessments of eel translocation efficacy and can also aid in the design of future tracking studies to more completely explore the downstream migration success of eel translocated into the highly productive waters of Lake Ontario.
Feasibility of a meditation intervention for stroke survivors and informal caregivers: a randomized controlled trial
Authors: Beauchamp Jennifer E. S. , Sharrief Anjail , Chaoul Alejandro , Casameni Montiel Tahani , Love Mary F. , Cron Stanley , Prossin Alan , Selvaraj Sudhakar , Dishman Deniz , Savitz Sean I. 
Published: January 2023

Abstract
Background
Depressive symptoms are a significant psychological complication of stroke, impacting both survivors and informal caregivers of survivors. Randomized controlled trials are needed to determine optimal non-pharmacological strategies to prevent or ameliorate depressive symptoms in stroke survivors and their informal caregivers.

Methods
A prospective, randomized, parallel-group, single-center, feasibility study. Participants were assigned to a 4-week meditation intervention or expressive writing control group. The intervention comprised four facilitator-led group meditation sessions, one session per week and building upon prior session(s). Descriptive statistics were used to examine the proportion of eligible individuals who enrolled, retention and adherence rates, and the proportion of questionnaires completed. Data were collected at baseline, immediately after the 4-week intervention period, and 4 and 8 weeks after the intervention period. Secondary analysis tested for changes in symptoms of depression (Center for Epidemiologic Studies-Depression [CES-D]), anxiety [State-Trait Anxiety Inventory for Adults (STAI)], and pain (Brief Pain Inventory-Short Form) in the intervention group via paired t tests. Linear mixed models were used to compare longitudinal changes in the measures between the groups. Intervention and trial design acceptability were preliminary explored.

Results
Seventy-one (77%) individuals enrolled and 26 (37%) completed the study (baseline and 8-week post-intervention visits completed). Forty-two (66%) participants completed baseline and immediate post-intervention visits. Mean questionnaire completion rate was 95%. The median meditation group session attendance rate for the intervention group was 75.0%, and the mean attendance rate was 55%. Non-significant reductions in CES-D scores were found. Paired t tests for stroke survivors indicated a significant reduction from baseline through week 8 in BPI-sf severity scores (p = 0.0270). Repeated measures analysis with linear mixed models for informal caregivers indicated a significant reduction in in STAI-Trait scores (F [3,16.2] = 3.28, p = 0.0479) and paired t test showed a significant reduction from baseline to week 4 in STAI-Trait scores (mean = − 9.1250, 95% CI [− 16.8060 to 1.4440], p = 0.0262). No between-group differences were found.

Conclusions
Future trials will require strategies to optimize retention and adherence before definitive efficacy testing of the meditation intervention.
Trial registration: ClinicalTrials.gov Identifier: NCT03239132. Registration date: 03/08/2017
Emotional processing in patients with single brain damage in the right hemisphere
Authors: Álvarez-Fernández Sonia , Andrade-González Nelson , Simal Patricia , Matias-Guiu Jordi A. , Gómez-Escalonilla Carlos , Rodriguez-Jimenez Roberto , Stiles Bryan J. , Lahera Guillermo 
Published: January 2023

Abstract
Background
The interest in the relationship between brain damage and social cognition has increased in recent years. The objectives of the present study were the following: (1) to evaluate and compare emotional facial recognition and subjective emotional experience in patients who have suffered a single ischemic stroke in the right hemisphere (RH) and in healthy people, (2) to analyze the relationship between both variables in both groups of subjects, and (3) to analyze the association between the cerebral location of the stroke and these two variables.

Methods
Emotional facial recognition and the subjective emotional experience of 41 patients who had suffered a single ischemic stroke in the RH and 45 volunteers without previous cerebrovascular pathology were evaluated.

Results
Brain damaged patients performed lower in facial emotional recognition and had a less intense subjective emotional response to social content stimuli compared to healthy subjects. Likewise, among patients with RH ischemic stroke, we observed negative associations between facial recognition of surprise and reactivity to unpleasant images, and positive associations between recognition of disgust and reactivity to pleasant images. Finally, patients with damage in the caudate nucleus of the RH presented a deficit in the recognition of happiness and sadness, and those with damage in the frontal lobe exhibited a deficit in the recognition of surprise, compared to those injured in other brain areas.

Conclusions
Emotional facial recognition and subjective emotional experience are affected in patients who have suffered a single ischemic stroke in the RH. Professionals caring for stroke patients should improve their understanding of the general condition of affected persons and their environment, assess for risk of depression, and facilitate their adaptation to work, family, and social environments.
Comparison of transcriptome profiles between medulloblastoma primary and recurrent tumors uncovers novel variance effects in relapses
Authors: Okonechnikov Konstantin , Federico Aniello , Schrimpf Daniel , Sievers Philipp , Sahm Felix , Koster Jan , Jones David T. W. , von Deimling Andreas , Pfister Stefan M. , Kool Marcel , Korshunov Andrey 
Published: January 2023

AbstractNowadays medulloblastoma (MB) tumors can be treated with risk-stratified approaches with up to 80% success rate. However, disease relapses occur in approximately 30% of patients and successful salvage treatment strategies at relapse remain scarce. Acquired copy number changes or TP53 mutations are known to occur frequently in relapses, while methylation profiles usually remain highly similar to those of the matching primary tumors, indicating that in general molecular subgrouping does not change during the course of the disease. In the current study, we have used RNA sequencing data to analyze the transcriptome profiles of 43 primary-relapse MB pairs in order to identify specific molecular features of relapses within various tumor groups. Gene variance analysis between primary and relapse samples demonstrated the impact of age in SHH-MB: the changes in gene expression relapse profiles were more pronounced in the younger patients (< 10 years old), which were also associated with increased DNA aberrations and somatic mutations at relapse probably driving this effect. For Group 3/4 MB transcriptome data analysis uncovered clear sets of genes either active or decreased at relapse that are significantly associated with survival, thus could be potential predictive markers. In addition, deconvolution analysis of bulk transcriptome data identified progression-associated differences in cell type enrichment. The proportion of undifferentiated progenitors increased in SHH-MB relapses with a concomitant decrease of differentiated neuron-like cells, while in Group 3/4 MB relapses cell cycle activity increases and differentiated neuron-like cells proportion decreases as well. Thus, our findings uncovered significant transcriptome changes in the molecular signatures of relapsed MB and could be potentially useful for further clinical purposes.
Application of hyperspectral imaging technology to digitally protect murals in the Qutan temple
Authors: Gao Zhenhua , Du Mingyi , Cao Ning , Hou Miaole , Wang Wanfu , Lyu Shuqiang 
Published: January 2023

Abstract
Hyperspectral imaging technology is a research hotspot in the field of cultural heritage protection. It can be used to quickly and noninvasively obtain detailed spectral information from the surfaces of cultural relics of different categories. We can intuitively analyse pigment compositions, line characteristics, painting skills and patterns using spectral information. Hyperspectral imaging has high scientific significance and application value for the protection, restoration and research of ancient murals and other cultural relics. In this study, a mural from Daheitian hall in the Qutan temple, Qinghai Province, China, was used as a sample. The hyperspectral data were acquired and analysed for several purposes. Pigment spectral matching and abundance inversion were carried out to obtain the pigment distribution. These data were enhanced by continuum removal and histogram stretching to obtain hidden information. The dark channel prior, Criminisi and Retinex methods were used to virtually restore the image of the mural. The results indicated that by using hyperspectral imaging data, the constructed pure pigment spectrum library and suitable approaches, the types and distributions of mural pigments can be quantitatively analysed, and the lines in murals can be extracted. Hyperspectral images are helpful for identifying information hidden by pigments or surface materials. Mural images can be enhanced, and hidden information can be highlighted using enhancement methods, such as continuum removal and histogram linear stretching. In addition, hyperspectral imaging data have unique advantages in the restoration of mural images, and the combination of defogging methods and image inpainting algorithms can realize the virtual restoration of mural images. In brief, hyperspectral imaging technology was found to have a highly favourable effect on pigment analysis, line extraction, information enhancement, hidden information extraction and the virtual restoration of ancient murals.
Understanding the microbial fibre degrading communities & processes in the equine gut
Authors: Wunderlich Georgia , Bull Michelle , Ross Tom , Rose Michael , Chapman Belinda 
Published: January 2023

AbstractThe equine gastrointestinal tract is a self-sufficient fermentation system, housing a complex microbial consortium that acts synergistically and independently to break down complex lignocellulolytic material that enters the equine gut. Despite being strict herbivores, equids such as horses and zebras lack the diversity of enzymes needed to completely break down plant tissue, instead relying on their resident microbes to carry out fibrolysis to yield vital energy sources such as short chain fatty acids. The bulk of equine digestion occurs in the large intestine, where digesta is fermented for 36–48 h through the synergistic activities of bacteria, fungi, and methanogenic archaea. Anaerobic gut dwelling bacteria and fungi break down complex plant polysaccharides through combined mechanical and enzymatic strategies, and notably possess some of the greatest diversity and repertoire of carbohydrate active enzymes among characterized microbes. In addition to the production of enzymes, some equid-isolated anaerobic fungi and bacteria have been shown to possess cellulosomes, powerful multi-enzyme complexes that further enhance break down. The activities of both anaerobic fungi and bacteria are further facilitated by facultatively aerobic yeasts and methanogenic archaea, who maintain an optimal environment for fibrolytic organisms, ultimately leading to increased fibrolytic microbial counts and heightened enzymatic activity. The unique interactions within the equine gut as well as the novel species and powerful mechanisms employed by these microbes makes the equine gut a valuable ecosystem to study fibrolytic functions within complex communities. This review outlines the primary taxa involved in fibre break down within the equine gut and further illuminates the enzymatic strategies and metabolic pathways used by these microbes. We discuss current methods used in analysing fibrolytic functions in complex microbial communities and propose a shift towards the development of functional assays to deepen our understanding of this unique ecosystem.
In silico identification of prospective virulence factors associated with candidiasis in Meyerozyma guilliermondii strain SO from genome dataset
Authors: Zainudin Robiatul Azilah , Sabri Suriana , Salleh Abu Bakar , Abu Arpah , Khairuddin Raja Farhana Raja , Oslan Siti Nurbaya 
Published: January 2023

Abstract
Background
Meyerozyma guilliermondii is a prospective yeast that has extensively contributed to the biotechnology sector. In 2015, M. guilliermondii strain SO which was isolated from spoiled orange has successfully been developed as an inducer-free expression system and attained a significant impact in producing industrially important recombinant proteins. The species possesses high similarity to Candida albicans which may cause candidiasis. The industrial-benefiting M. guilliermondii strain SO has been underexplored for its virulence status. Thus, this study aimed to document the potential virulence factors through the comprehensive in silico analysis of M. guilliermondii strain SO genome. This analysis demonstrated the molecular characterization which could distinguish the pathogenicity status of M. guilliermondii.

Results
The genome data were generated from Illumina HiSeq 4000 sequencing platform and assembled into 51 scaffolds successfully accumulating a genome size of 10.63 Mbp. These enclosed 5,335 CDS genes and 5,349 protein sequences with 43.72% GC content. About 99.29% of them were annotated to public databases. Komagataella phaffii, Saccharomyces cerevisiae and the reference strain of M. guilliermondii (ATCC 6260) were used as the controls. They were compared with our in-house strain SO to identify the consensus domain or subdomain which could putatively be considered as virulence factors. Candida albicans was used as the pathogenic model. Hence, hidden Markov model against strain SO proteome had identified secreted aspartic proteases (SAP), phospholipase C (PLC) and phospholipase D (PLD) with an E-value of 2.4e−107, 9.5e−200 and 0.0e+00, respectively, in resemblance of C. albicans. The topology of the phylogenetic analysis indicated that these virulence factors in M. guilliermondii strain SO and C. albicans branched from the same node and clustered together as a clade, signifying their molecular relatedness and congeneric among these species, subsequently proposing the virulence status of M. guilliermondii.

Conclusion
The SAP, PLC and PLD genes’ features that were significant in expressing determinants of pathogenicity were successfully identified in M. guilliermondii strain SO genome dataset, thus concluding the virulency of this species. On account of this finding, the strategy of gene knockout through CRISPR-Cas9 or homologous recombination strategies is needed to engineer the feasible novel expression host system. Over and above, the genetically modified strain of M. guilliermondii allegedly may eradicate the risk of candidiasis infection.
The adult and pediatric palliative care: differences and shared issues
Authors: Chelazzi Cosimo , Villa Gianluca , Lanini Iacopo , Romagnoli Stefano , Latronico Nicola 
Published: January 2023

AbstractAdult and pediatric palliative care (PC) share common aims and ethical principles but differ in many organizational and practical aspects. The aim of this narrative review is to analyze these differences and focus on which key aspects of pediatric palliative care could integrate adult services for a better care of suffering patients.Interventions which are peculiar of pediatric PC respect to adult PC include: an earlier referral to the PC service to identify the needs and plan the interventions at an earlier stage of the disease; consequently, a more systematic cooperation with the disease-specific physicians to reduce the burden of treatments; a better integration with the community and the social surroundings of the patients, to prevent social isolation and preserve their social role; a more dynamic organization of the PC services, to give patients the chance of being stabilized at in-hospital or residential settings and subsequently discharged and cared at home whenever possible and desired; the implementation of respite care for adults, to help the families coping with the burden of the disease of their beloved and promote the home-based PC.This review underlines the relevance of some key-aspects of pediatric PC that can be beneficial also within PC of adults. Its findings give the chance for a more dynamic and modern organization of adult PC services and may serve as a basis of future research for new interventions.