bluuebunny/crossref_metadata_2025_split huggingface dataset:
Biogenic Silver Nanoparticles and Stressors Generate Synergistic Growth
Inhibition in Candida Species through Cell Wall Damage, Osmotic Stress,
and Oxidative Stress
Authors: Cervantes-Chávez José Antonio , García-Bouchot Gastón , García-Gutiérrez Nataly , Vergara- Castañeda Hayde Azeneth , Nava-Mendoza Rufino , Luna-Bárcenas Gabriel , Elizalde-Peña Eduardo Arturo , Esquivel-Naranjo Edgardo Ulises , Landeros-Jaime Fidel , Rojas-Avelizapa Norma Gabriela , Pool Héctor 
Published: March 2023

Background:
The need to combat and reduce the incidence, virulence, and drug resistance
of species belonging to Candida genus, has led to the development of new strategies.
Nanotechnology, through the implementation of nanomaterials, has emerged as an infallible tool
to treat various diseases caused by pathogens, where its mechanisms of action prevent the development
of undesirable pharmacological resistance.

Objective:
The antifungal activity and adjuvant properties of biogenic silver nanoparticles in different
Candida species (C. parapsilosis, C. glabrata, and C. albicans) are evaluated.

Methods:
The biogenic metallic nanoparticles were developed by quercetin-mediated biological
synthesis. The physicochemical properties were studied by light scattering, electrophoretic mobility,
UV-vis and infrared spectroscopy, and transmission electron microscopy. The elucidation of
mechanisms of antifungal action was carried out under stress conditions in Candida species at the
cell wall and response to oxidative stress.

Results:
Small silver nanoparticles (≈ 16.18 nm) with irregular morphology, and negative surface
electrical charge (≈ -48.99 mV), were obtained through quercetin-mediated biosynthesis. Infrared
spectra showed that the surface of silver nanoparticles is functionalized with the quercetin molecule.
The antifungal activity of biogenic nanoparticles had efficacy in the following trend C. glabrata
≥ C. parapsilosis > C. albicans. Biogenic nanoparticles and stressors showed synergistic
and potentiated antifungal effects through cell damage, osmotic stress, cell wall damage, and oxidative
stress.

Conclusion:
Silver nanoparticles synthesized by quercetin-mediated biosynthesis could be implemented
as a powerful adjuvant agent to enhance the inhibition effects of diverse compounds
over different Candida species.
The Characteristics of Entorhinal Cortex Functional Connectivity in
Alzheimer’s Disease Patients with Depression
Authors: Zhu Haokai , Zhu Hong , Liu Xiaozheng , Wei Fuquan , Li Huichao , Guo Zhongwei 
Published: March 2023

Background:
Depression is one of the most common neuropsychiatric symptoms of Alzheimer’s disease (AD) which decreases the life quality of both patients and caregivers. There are currently no effective drugs. It is therefore important to explore the pathogenesis of depression in AD patients.

Objective:
The present study aimed to investigate the characteristics of the entorhinal cortex (EC) functional connectivity (FC) in the whole brain neural network of AD patients with depression (D-AD).

Methods:
Twenty-four D-AD patients, 14 AD patients without depression (nD-AD), and 20 healthy controls underwent resting-state functional magnetic resonance imaging. We set the EC as the seed and used FC analysis. One-way analysis of variance was used to examine FC differences among the three groups.

Results:
Using the left EC as the seed point, there were FC differences among the three groups in the left EC–inferior occipital gyrus. Using the right EC as the seed point, there were FC differences among the three groups in the right EC–middle frontal gyrus, –superior parietal gyrus, –superior medial frontal gyrus, and –precentral gyrus. Compared with the nD-AD group, the D-AD group had increased FC between the right EC and right postcentral gyrus.

Conclusion:
Asymmetry of FC in the EC and increased FC between the EC and right postcentral gyrus may be important in the pathogenesis of depression in AD.
Sleep Characteristics in Older Adults with Different Levels of Risk for
Dementia: A Cross-sectional Study
Authors: Huang Xiuxiu , Zhang Shifang , Fang Yuxi , Zhao Xiaoyan , Cao Ting , Sun Yongan , Wan Qiaoqin 
Published: March 2023

Background:
Sleep problems are very prevalent in older adults, especially in those at risk for dementia. But the relationships between sleep parameters and subjective or objective cognitive decline are still inconclusive.

Aim:
The study aimed to investigate the self-reported and objectively measured sleep characteristics in older adults with mild cognitive impairment (MCI) and subjective cognitive decline (SCD).

Methods:
This study adopted a cross-sectional design. We included older adults with SCD or MCI. Sleep quality was measured separately by the Pittsburgh sleep quality index (PSQI) and ActiGraph. Participants with SCD were divided into low, moderate, and high levels of SCD groups. Independent samples T-tests, one-way ANOVA, or nonparametric tests were used to compare the sleep parameters across groups. Covariance analyses were also performed to control the covariates.

Results:
Around half of the participants (45.9%) reported poor sleep quality (PSQI＜7), and 71.3% of participants slept less than 7 hours per night, as measured by ActiGraph. Participants with MCI showed shorter time in bed (TIB) (p＜0.05), a tendency of shorter total sleep time (TST) at night (p = 0.074) and for each 24-hour cycle (p = 0.069), compared to those with SCD. The high SCD group reported the highest PSQI total score and longest sleep latency than all the other three groups (p＜0.05). Both the MCI and high SCD groups had shorter TIB and TST for each 24-hour cycle than the low or moderate SCD groups. Besides, participants with multiple-domain SCD reported poorer sleep quality than those with single-domain SCD (p＜0.05).

Conclusion:
Sleep dysregulation is prevalent in older adults with a risk for dementia. Our findings revealed that objectively measured sleep duration might be an early sign of MCI. Individuals with high levels of SCD demonstrated poorerself-perceived sleep quality and deserved more attention. Improving sleep quality might be a potential target to prevent cognitive decline for people with a risk for dementia.
Wnt/β-catenin Signaling Inhibitors
Authors: Zhang Xun , Dong Nazhen , Hu Xiaoyan 
Published: March 2023

Abstract:The Wnt/β-catenin signaling pathway plays a crucial role in the development, tissue ho-meostasis, angiogenesis, and carcinogenesis of cancer. Mutations and excessive activation of the Wnt/β-catenin signaling pathway in cancer cells and cancer stem cells lead to drug resistance and recurrence of cancer in patients treated with conventional chemotherapy and radiotherapy. Upregu-lation of proangiogenic factors is persistently induced by hyperactivated Wnt/β-catenin signaling during tumor angiogenesis. Furthermore, mutations and hyperactivated Wnt/β-catenin signaling are associated with worse outcomes in several human cancers, including breast cancer, cervical cancer, and glioma. Therefore, mutations and hyperactivation of Wnt/β-catenin signaling create challenges and limitations in cancer treatment. Recently, in silico drug design as well as high-throughput as-says and experiments have demonstrated the promising anticancer efficacy of chemotherapeutics, such as blocking the cancer cell cycle, inhibiting cancer cell proliferation and endothelial cell angi-ogenesis, inducing cancer cell apoptosis, removing cancer stem cells, and enhancing immune re-sponses. Compared to conventional chemotherapy and radiotherapy, small-molecule inhibitors are considered the most promising therapeutic strategy for targeting the Wnt/β-catenin signaling path-way. Herein, we review the current small-molecule inhibitors of the Wnt/β-catenin signaling path-way, focusing on Wnt ligands, Wnt receptors, the β-catenin destruction complex, ubiquitin ligases and proteasomal destruction complex, β-catenin, β-catenin-associated transcriptional factors and co-activators, and proangiogenic factors. We describe the structure, mechanisms, and functions of these small molecules during cancer treatment in preclinical and clinical trials. We also review sev-eral Wnt/β-catenin inhibitors reported to exhibit anti-angiogenic effects. Finally, we explain various challenges in the targeting of the Wnt/β-catenin signaling pathway in human cancer treatment and suggest potential therapeutic approaches to human cancer.
Research Progress of 3D Printing Technology for Pharmaceutical
Preparation
Authors: Tang Liqiang , Xie Baocheng 
Published: March 2023

Abstract:
Pharmaceutical preparation is a kind of finished pharmaceutical product made by combining
raw materials with various auxiliary materials in a certain form. At present, the field of
pharmaceutical preparations can meet most drug needs, but there are some limitations. It is difficult
to realize the production of personalized preparations. Because 3D printing technology has the ability
of precise dose control and flexible shape customization, it can realize precise control of drug
dosage, release behavior and local targeting in pharmaceutical preparations. Therefore, in medicine,
3D printing technology is increasingly used in the field of pharmaceutical preparations. 3D printing
technology provides an important means for new drug printing and personalized drug customization
of pharmaceutical preparations in the medical field. The 3D printing technology of drugs will inject
fresh vitality into individualized drug delivery. Therefore, the development trend of 3D printing
technology for pharmaceutical preparations has attracted more and more attention. In order to optimize
the wide application of 3D printing technology in pharmaceutical preparation, 3D printing
technology such as inkjet 3D printing technology, extrusion 3D printing technology and laser 3D
printing technology were studied. In this paper, the selection, classification and introduction of 3D
printing technology such as inkjet 3D printing technology, extrusion 3D printing technology and
laser 3D printing technology in pharmaceutical preparations are reviewed. Through the investigation
of various patents of 3D printing technology applied to pharmaceutical preparation in medicine, this
paper summarizes and analyzes the main problems of 3D printing technology applied to pharmaceutical
preparations, such as printing stability, production quality, etc. In addition, the development
trend of 3D printing technology is also discussed. Optimization of various 3D printing technologies
applied to pharmaceutical preparation in medicine is beneficial to improve printing stability and
production quality in medicine. More related patents will be invented in the future.
Sorafenib Loaded Resealed Erythrocytes for the Treatment of Hepatocellular
Carcinoma
Authors: Desai Raj M. , Desai Neha , Momin Munira , Bhatt Lokesh Kumar 
Published: March 2023

Background:
This study aims to formulate and characterize sorafenib-loaded resealed erythrocytes
(SoRE) and investigate their anticancer activity in a rat model of hepatocellular carcinoma.

Methods:
SoRE were prepared by hypotonic dialysis of red blood cells obtained from Wistar rats using
a range of drug-containing dialysis mediums (2-10 mg/ml) and osmosis time (30-240 mins). Optimized
SoRE (8 mg/mL and 240 mins) were characterized for size, morphology, stability, entrapment
efficiency, in vitro release profiles, and in vivo efficacy evaluations. For efficacy studies, optimized
SoRE were intravenously administered to Wistar rats having hepatocellular lesions induced by aflatoxin
B and monitored for in vivo antineoplastic activity.

Results:
The amount of sorafenib entrapped was directly proportional to the drug concentration in the
dialysis medium and duration of osmosis; highest for 10 mg/mL and 240 minutes and lowest for 2
mg/mL and 30 minutes, respectively. Optimized SoRE were biconcave with a size of 112.7 nm and
zeta potential of -11.95 ± 2.25 mV. Osmotic and turbulence fragility were comparable with native
erythrocytes.

Conclusion:
Drug release follows the first-order pattern. In vivo investigations reveal better anticancer
activity of SoRE formulation compared to sorafenib standard preparation. Resealed erythrocytes loaded
with sorafenib displayed first-order in vitro release and promising anticancer activity in a rat model
of hepatocellular carcinoma.
Research Status and Emerging Trends in Virtual Reality Rehabilitation: Bibliometric and Knowledge Graph Study
Authors: Fan Ting , Wang Xiaobei , Song Xiaoxi , Zhao Gang , Zhang Zhichang 
Published: March 2023

Background
Virtual reality (VR) technology has been widely used in rehabilitation training because of its immersive, interactive, and imaginative features. A comprehensive bibliometric review is required to help researchers focus on future directions based on the new deﬁnitions of VR technologies in rehabilitation, which reveal new situations and requirements.

Objective
Herein, we aimed to summarize effective research methods for and potential innovative approaches to VR rehabilitation by evaluating publications from various countries to encourage research on efficient strategies to improve VR rehabilitation.

Methods
The SCIE (Science Citation Index Expanded) database was searched on January 20, 2022, for publications related to the application of VR technology in rehabilitation research. We found 1617 papers, and we created a clustered network, using the 46,116 references cited in the papers. CiteSpace V (Drexel University) and VOSviewer (Leiden University) were used to identify countries, institutions, journals, keywords, cocited references, and research hot spots.

Results
A total of 63 countries and 1921 institutes have contributed publications. The United States of America has taken the leading position in this field; it has the highest number of publications; the highest h-index; and the largest collaborative network, which includes other countries. The reference clusters of SCIE papers were divided into the following nine categories: kinematics, neurorehabilitation, brain injury, exergames, aging, motor rehabilitation, mobility, cerebral palsy, and exercise intensity. The research frontiers were represented by the following keywords: video games (2017-2021), and young adults (2018-2021).

Conclusions
Our study comprehensively assesses the current research state of VR rehabilitation and analyzes the current research hot spots and future trends in the field, with the aims of providing resources for more intensive investigation and encouraging more researchers to further develop VR rehabilitation.
Vertical Integration of Electronic Health Records in Medical Consortiums: Dynamic Modeling Approach Based on the Evolutionary Game Theory
Authors: Tian Shenghu , Chen Yu 
Published: March 2023

Background
China has continuously issued policies to speed up the interconnection, mutual recognition, sharing of medical information systems, and data integration management across regions and institutions. However, the vertical integration of electronic health records (EHRs) within the medical consortium is hampered by “poor mechanism and insufficient motivation” and the phenomenon of “free riding” among participating medical institutions, which makes the integration less effective.

Objective
We hope to clarify the game mechanism of stakeholders in the vertical integration of EHRs, and put forward targeted policy suggestions for improvement.

Methods
We constructed the “government-hospital-patient” tripartite evolutionary game model based on the detailed analysis of the research problems and their assumptions. We then simulated the game strategies and outcomes of each participant using the system dynamics approach to reveal the long-term strategy evolution mechanism of the core participants in the vertical integration of EHRs in the medical consortium, as well as the influencing factors and action mechanisms of each party’s strategy evolution to provide references for improving relevant policies.

Results
The evolutionary game system could eventually reach an optimal equilibrium, but in areas where the government was required to be in a dominant position, patient supervision was necessary to have a positive role, while a reasonable reward and punishment mechanism can promote active participation of hospitals.

Conclusions
The effective way to achieve the goal of vertical integration of EHRs in the medical consortium is to build a multiagent coordination mechanism under the guidance of the government. Meanwhile, it is necessary to establish a scientific integration performance evaluation mechanism, a reward and punishment mechanism, and a benefit distribution mechanism to promote the healthy development of vertical integration of EHRs in medical consortiums.
Screening for Media Use in the Emergency Department Among Young Australians: Cross-sectional Study
Authors: Dullur Pravin , Joseph Joanne , Diaz Antonio Mendoza , Lin Ping-I , Jairam Rajeev , Davies Rhian , Masi Anne , Shulruf Boaz , Eapen Valsamma 
Published: March 2023

Background
Research on problematic internet use has largely adhered to addiction paradigms, possibly impeding the identification of specific internet behaviors related to psychopathology. This study presents a novel approach to screening for specific problematic internet behaviors by using a new measure, the emergency department media use screener (EDMUS).

Objective
The purpose of this study was to identify patterns of internet use in young people presenting with mental health concerns to the emergency department (ED), ascertain associations with their mental health, and evaluate whether the EDMUS can be used to predict subsequent ED presentations within 3 months.

Methods
This cross-sectional retrospective study of Australian young people (N=149, aged 11-25 years; female: n=92, 61.7%) sought to use the EDMUS, a 24-item questionnaire, to identify problematic internet behaviors, including accessing or posting prosuicidal or proeating disorder content, cyberbullying, and inappropriate digital content. Data on each person’s mental health were extracted from electronic medical records to look for associations with EDMUS responses and ED re-presentation over 3 months. EDMUS items were grouped into clusters for analysis using chi-square tests, binary logistic regression, and path analyses.

Results
Sharing suicidal digital content was the most common problematic internet use pattern identified by the EDMUS. However, this did not correlate with having a prior mental health diagnosis or predict readmission. Most participants had families with a concern for their internet use; however, this was less likely in participants with a diagnosis of personality disorder. Diagnoses of personality disorder or posttraumatic stress disorder were independent predictors of readmission (P=.003; P=.048).

Conclusions
Although a history of complex psychopathology increases the likelihood of subsequent ED presentations, its links to internet use–related behaviors are still unclear. The EDMUS has potential for identifying young people who are most vulnerable to problematic internet behaviors and offers the opportunity for early intervention and potential prevention of more entrenched difficulties.
KEAMANAN SURAT UTANG NEGARA YANG DIJUAL SECARA LELANG DITINJAU DARI ASAS AKUNTABILITAS
Authors: Slamet arifin  
Published: March 2023

ABSTRACT

The government auctioned seven series of Government Securities on March 29, 2022, the auction was conducted so that the government would obtain funds to finance the 2022 State Budget, by conducting an auction of Government Securities for the SPN03220629 (new issuance), SPN 12230330 (new issuance), FR0090 (reopening) series. FR0089 (reopening). The purpose of a person participating in the auction of Sovereign Debt Instruments is to invest, considering that Government Securities are included in bonds, which is one of the products traded in the capital market as an investment scope. Sovereign Debt Instruments are securities in the form of debt acknowledgments that are guaranteed payment of interest and principal by the Republic of Indonesia according to their validity period, so everything in the object of Government Securities must be accountable by the government, both from the legal aspect and from the economic aspect.
The research method of this writing uses normative juridical research methods. The approach used in this study uses an analytical descriptive analysis method regarding the security of Government Securities sold at auction in terms of the principle of accountability. The technique of collecting legal materials is carried out through library research and analysis of library materials, as well as internet browsing studies including articles and scientific journals related to this research. After the legal materials have been collected, they are then analyzed using descriptive analytical and critical techniques.
The Government Securities Auction is conducted using an auction system organized by Bank Indonesia, which is open (open auction), using the multi-price method. Auction winners who submit non-competitive bids will pay according to the weighted average yield of the winning competitive bids. In the application of the principle of accountability, the government must provide certainty and security guarantees for the object of the auction of Government Securities, both juridically and economically. Bearing in mind that Government Securities are part of assets, in which assets there are potential risks in them, which can potentially harm and/or benefit the parties who control the object.
Witch Trials in Seventeenth-Century Târgu Mureş
Authors: GYÖRGY Árpád Botond 
Published: March 2023

The witch trials from Târgu-Mures are typical ones and fit together with the other cases from the early modern period. We can identify the same processes and elements as historiography and ethnography had described. On the other hand, these cases are slightly different because these witch trials were less bloody than usually in the seventeenth century. In the Hungarian Kingdom, the zenith of the witch hunts was in the middle of the eighteenth century. Perhaps the intensity of witch hunts in a medium town was smaller. This paper aims to describe and analyse the witch cases from the seventeenth-century Târgu Mures. These cases are not that remarkable, but we can gain insight into the juridical processes through them.
Rezumat: Procesele de vrăjitorie din Târgu-Mureş sunt tipice şi se potrivesc printre celelalte cazuri din perioada modernă timpurie. Putem identifica aceleaşi procese şi elemente ca cele descrise de istoriografie şi etnografie. Pe de altă parte, aceste cazuri sunt puțin diferite, deoarece aceste procese de vrăjitoare sunt mai puțin sângeroase ca de obicei în secolul al 17-lea. În Regatul Ungariei, zenitul vânătorilor de vrăjitoare a fost la mijlocul secolului al 18-lea. Poate că intensitatea vânătorilor de vrăjitoare într-un oraş mediu a fost mai mică. Studiul de față doreşte să descrie şi să analizeze cazurile de vrăjitorie din secolul al 17-lea din Târgu Mureş. Aceste cazuri nu sunt atât de remarcabile, dar prin ele putem obține o perspectivă asupra proceselor juridice.
Cuvinte-cheie: Târgu Mureş, procese de vrăjitoare, secolul al 17-lea, protocoale