bluuebunny/crossref_metadata_2025_split huggingface dataset:
A novel method for detection of Cas9 gRNAs using a fluorophore-labeled DNA oligo
Authors: Bandara Ranmal Avinash , Zhou Zhichang Peter , Chen Ziyan Rachel , Duan Rongqi , Davidson Alan Richard , Wong Amy P , Hu Jim 
Published: February 2025

AbstractClustered regularly interspaced short palindromic repeats (CRISPR) associated protein 9 (Cas9) has become one of the most important gene editing tools. CRISPR guide RNA (gRNA) serves as an important component in guiding Cas proteins to a target site in gene editing processes. For effective gene editing or site specific gene integration, levels of gRNA present in a target cell are critical. Especially, for prime editing, even the ratio of different gRNAs present in target cells has a major effect on gene editing efficiency. Therefore, having a convenient, highly sensitive method for direct detection of gRNAs can improve editing design and optimize targeting efficiency. Here, we have developed a convenient, highly sensitive method for direct detection of gRNAs, which may be used to improve editing design and optimize targeting efficiency.
Reward-based prosocial choices in mice
Authors: Esteve-Agraz Joan , Rodríguez-Milan Víctor Javier , Márquez Cristina 
Published: February 2025

AbstractProsocial behaviors, actions that benefit others, are an essential part of the social life of humans and other animals, by promoting bonding and cohesion among individuals and groups. Here we present a new behavioural paradigm to assess prosociality in food foraging contexts in laboratory mice, based in our paradigm previously developed for rats. In this task, the decision-maker can choose between two options, one that will only provide rewards to itself (selfish choice) or one that will reward both itself and its cagemate (prosocial choice). Our work reveals that prosocial choices in male mice are not widespread and are only observed in a small proportion of animals. Using detailed analysis of behavior, we describe that recipients of help express different social cues in prosocial and selfish trials, but decision makers do not take them into account to guide their choices. Furthermore, we assess how the level of individual training and the physical layout of the paradigm might affect the performance in this social task. Only those mice with increased social attention (16% of the animals) display prosocial preferences, suggesting these to be rooted in similar behavioural factors and social interactions that we previously described in other work with rats.
Evaluating the impact of staff training on “personality disorder” on attitudes, knowledge and confidence over time
Authors: Myklebust Hege , Gaffney Hannah , Parkinson Sarah 
Published: February 2025

Purpose
Research demonstrates that health-care staff can hold negative attitudes toward people diagnosed with “Personality Disorders” (PdxPD), which can negatively impact health-care outcomes. Evidence for staff training is inconsistent with limited demonstration of sustained impact. This study aims to evaluate whether a new, brief training session can deliver similar results to longer training packages and sustain positive change 6- and 12-months after training (Black et al., 2011).

Design/methodology/approach
A total of 428 health-care staff attended the 3-hour training session during the study period. Questionnaires assessing knowledge, confidence and attitudes were administered pre- and post-training and at 6- and 12-month follow-up.

Findings
Brief training significantly improved knowledge of PdxPD (p < 0.001), attitudes towards PdxPD (p < 0.001) and staff confidence (p < 0.001) working with PdxPD after training. Feedback showed high ratings of training usefulness and quality of training delivery. Improvements in knowledge, attitudes and confidence were maintained over 12 months. However, gains in knowledge were not maintained at six months.

Research limitations/implications
The study demonstrates positive outcomes from short training which can lead to significant resource and clinical time savings.

Originality/value
Findings suggest that the brief training session can achieve similar outcomes to more intensive training evaluated elsewhere. To the best of the authors’ knowledge, this study is the first to consider maintenance of outcomes at 12 months for foundational PdxPD training. The staff sample was broad and not limited to staff working in specific settings or professions.
Optimal maintenance and spare part policy for an urban micro wind power system
Authors: Lin Hongjie , Qi Faqun , Liu Yuxin , Chen Xiang , Zha Wenfei 
Published: February 2025

PurposeThis paper aims to develop an optimal maintenance and spare parts policy for an urban micro wind power system, focusing on two urban micro wind farms (UMWF). The reliability and efficiency of these systems are sought to be enhanced by considering the relationship between urban wind parameters and wind turbine degradation.Design/methodology/approachA proportional hazards (PH) model is utilized to describe how urban wind conditions impact turbine degradation. The maintenance strategy includes preventive maintenance (PM), corrective maintenance (CM) and opportunistic maintenance (OM). A multi-objective optimization algorithm is developed to optimize the joint policy of OM plans and spare parts resource allocation.FindingsThe proposed maintenance and spare parts policy effectively balances the trade-offs between PM, CM and OM strategies. Numerical experiments demonstrate that the policy improves the reliability of UMWF, reducing downtime and maintenance costs while ensuring the availability of spare parts when needed. The results show a significant enhancement in system performance compared to traditional maintenance approaches.Originality/valueA novel maintenance policy and spare parts management approach for urban micro wind power systems is proposed. A multi-objective optimization algorithm is developed to optimize the OM schedule and maintenance spare parts resource management strategy for wind farms in urban wind environments.
Owing to interactivity: a meta-analysis on consumer-brand responses in the digital context
Authors: Fan Xiaojun , Li Huiyao , Jiang Xinyu 
Published: February 2025

PurposeInteractivity is the key to developing digital branding. However, existing research on brand interactivity outcomes is inconsistent and fragmented, lacking a systematic empirical exploration of its effects on consumer responses in the digital context.Design/methodology/approachDrawing upon the cognition-affection-conation (CAC) framework as our theoretical compass, a meta-analysis was conducted to synthesize and analyze empirical evidence from 144 samples involving 57,952 participants to assess how and when digital brand interactivity influences consumers’ multilevel responses.FindingsOur narrative unfolds with digital brand interactivity as the catalyst, fostering positive consumer behaviors – brand loyalty and purchase intention – through a sequential dance of cognitive mindset shifts (brand experience, engagement and attitude) and affective resonance (trust and emotional attachment). A moderation analysis adds depth, revealing stronger effects in B2C settings for lesser-known brands with hedonic interaction content and among individuals with a collectivist orientation.Practical implicationsOur findings serve as a roadmap for targeted digital marketing strategies, guiding brands, consumers and contextual aspects to optimize the performance of digital branding by harnessing the full potential of digital interactivity.Originality/valueThis study introduces a framework combining CAC and brand-consumer psychology to understand how interactivity affects consumer responses in digital contexts. It delves into dynamic shifts moderated by brand characteristics, consumer traits and contextual factors, offering a holistic view of digital branding’s impact on interactive marketing.
Unleashing supply chain innovation and frugal innovation: examining the interaction between institutional pressures and supply chain analytics adoption
Authors: Al-Omoush Khaled Saleh , Fernández-Caparrós Miguel Reinoso , Lassala Carlos , Ribeiro-Navarrete Samuel 
Published: February 2025

PurposeThis study investigates the effects of institutional pressures on the adoption of supply chain analytics (SCA) and its subsequent impact on supply chain (SC) innovation and frugal innovation. It also examines the correlation between SC innovation and frugal innovation.Design/methodology/approachThe study collected data from manufacturing companies in Jordan. PLS path analysis is applied.FindingsThe PLS path analysis showed that coercive pressures did not significantly affect the outcome, but both normative and mimetic ones were found to have significant effects on SCA adoption. The study also shows that SCA plays a significant role in SC innovation and frugal innovation. Furthermore, it demonstrates that SC innovation significantly impacts frugal innovation.Originality/valueOverall, these findings provide valuable theoretical implications to the literature by advancing our understanding of the interactive relationships between institutional pressures, SCA, SC innovation and frugal innovation. Understanding the role that institutional pressures play in SCA adoption and its subsequent impact on SC innovation and frugal innovation enables organizations to strategically position themselves for competitive advantage and sustainable growth within the dynamic field of supply chain management.
Brand experiences and loyalty among young smartphone users: a serial mediation analysis
Authors: Gazi Md. Abu Issa , Ibrahim Md. , Masud Abdullah Al , Reza Syed Muhammod Ali 
Published: February 2025

PurposeThe current study aims to investigate how young smartphone users in Bangladesh relate their brand experiences to brand loyalty. In addition, we want to visualize the direct and mediating effects of brand satisfaction, brand love and brand advocacy in our model.Design/methodology/approachThe researchers examined the hypotheses by employing structural equation modeling (SEM) in AMOS and Decision Analyst STATS, version 2.0, with a sample size of 470 Bangladeshi smartphone users. The authors constructed the conceptual model by drawing upon both theoretical and empirical foundations. The researchers obtained data by utilizing an adopted and self-administered pre-structured questionnaire distributed via an online platform.FindingsThe results showed that brand experience greatly influences brand satisfaction, love, advocacy and loyalty, all of which have a significant impact on users’ brand loyalty across the country. The findings also suggested that the function of brand satisfaction as a critical mediator in the link between brand experience and brand loyalty was significant.Originality/valueThis experiment contributes to the body of knowledge by focusing on emotional brand attachments like brand satisfaction, love and advocacy and proposing that they can mediate experience and loyalty in the mobile market. The study also helps managers and executives better understand the primary drivers of smartphones, which are essential for generating and sustaining consumers’ happiness and loyalty in today’s highly competitive consumer market.
Recordkeeping for project management information system in public procurement: an action research
Authors: Rebuglio Massimo , Ottaviani Filippo Maria , De Marco Alberto 
Published: February 2025

Purpose
This study aims to examine the impact of laws, regulations and standards (i.e. the sources) on implementing a recordkeeping system in the public sector. It then provides an approach to set up a recordkeeping system in public procurement that complies with modern project management (PM) paradigms, information technology best practices and sources.

Design/methodology/approach
This study used the action research methodology to implement a recordkeeping system module within a PM information system for an Italian public procurement project. The study involved mapping the existing information flows, analyzing stakeholders’ needs and constraints and developing, testing and analyzing PM approaches and tools.

Findings
Regarding the impact of the sources, national laws set usability parameters and specify the content to store but often overlook storage methods and formats. Customs dictate data exchange formats, significantly affecting storage efficiency and retrieval, and are challenging to alter. Contracts outline content requirements and may standardize formats among parties. Standards establish foundational principles, ensuring records remain authentic, retrievable and usable over time. The proposed approach to address these constraints involves implementing information systems across multiple layers.

Practical implications
This research proposes a blueprint for effective recordkeeping in public procurement. This can lead to cost savings, reduced paperwork and faster access to critical data. In terms of proposed policies, it is also discussed how contracts could address these issues at the root by imposing suitable formats for PM data exchange.

Originality/value
The novelty of this study is associated with the proposed approach for designing and implementing recordkeeping systems that comply with laws, regulations and standards aligned with modern PM paradigms.
Conceptualizing and modeling firms’ price image in the business market
Authors: Ranjan Piyush 
Published: February 2025

PurposePrice image studies have dominated the consumer market but have been slower to evolve in the business market. This research aims to conceptualize and validate the firms’ price image (FPI) in the business market, as well as to develop a conceptual framework for exploring the importance of pricing capability (PC) in the formation of FPI and its influence on customer value (CV) and business performance (BP).Design/methodology/approachThe study conducted field interviews with business managers and customers of Indian small and medium-sized enterprises to operationalize and validate the FPI variable using a mixed method of qualitative and quantitative multivariate analyses. Partial least square structural equation modeling technique was applied to validate a conceptual model on a sample of 286 respondents acquired from both physical and digital questionnaire survey techniques.FindingsThe findings revealed that PC is positively linked to FPI, which in turn, significantly affects CV and BP. Moreover, CV serves as a partial mediator in the FPI–BP link. The research also offers a measurement scale consisting of eight items for assessing the FPI variable from the stakeholders’ perspective.Research limitations/implicationsThis research contributes to the literature on price image by refining and testing measures of FPI, and enriches the existing literature by understanding the theoretical relationships among PC, FPI, CV, and BP.Practical implicationsThe research reveals that developing PC is a useful tool for SMEs to build favorable FPI, which delivers superior CV and obtains improved BP. The findings can also assist SME managers to gain a sustainable competitive advantage by maintaining a favorable FPI.Originality/valueWhile a growing number of consumer market studies reveal that price image is strategically important for the retailers who own it, no study has explored the role of price image in the business market. This is the first study to explore price image at the firm level in the business market. This study further provides empirical evidence for the claim that PC influences the formation of a FPI and clarifies the relationship between FPI, CV and BP.
Species‐specific seasonal variations in thermal performance curves shape the direct and transgenerational vulnerability to marine heatwaves
Authors: Dinh Khuong V. , Vu Minh T. T. 
Published: February 2025

AbstractResearch Highlights: Sasaki, M., Finiguerra, M. & Dam, H.G. (2024). Seasonally variable thermal performance curves prevent adverse effects of heatwaves. Journal of Animal Ecology. https://doi.org/10.1111/1365‐2656.14221. Marine heatwaves (MHWs) emerge as a devastating stressor that can have direct and transgenerational effects on marine organisms. However, we know very little about how seasonal variations in thermal performance curves (TPCs) may help marine zooplankton cope with these direct and transgenerational effects of MHWs. In a recent study, Sasaki et al. (2024) combined field observations and simulated laboratory heatwave experiments, uncovering seasonal variations in TPCs for key fitness‐related traits, including egg and offspring production, hatching success and survivorship in two ecologically important copepod species Acartia tonsa and A. hudsonica. They discovered that the TPC of A. tonsa was highly seasonally variable, allowing them to maintain their thermal optimum of at least 5°C above the field temperature. The transgenerational effects of parental exposure to MHWs on the offspring were minor. In contrast, the TPC of A. hudsonica was relatively unchanged across seasons, suggesting that this species may be highly vulnerable to MHWs, especially during summer. These findings agree with distinct seasonal abundances of the two species in nature: A. hudsonica is primarily abundant during winter and spring while A. tonsa dominates the summer and fall. These findings enhance our understanding of how seasonal variations in TPCs can determine the vulnerability of marine species to heatwaves through direct and transgenerational effects, which are important for ecological risk assessments of marine ecosystems under a rapidly changing climate.
Triple gains: More production, less nitrogen and greater diversity from cropland reallocation in England and Wales
Authors: Almeida‐Furtado Murilo , Meuwissen Miranda P. M. , Ang Frederic 
Published: February 2025

AbstractAgricultural production is the main driver of nitrogen pollution and diversity loss. This study assesses the potential of cropland reallocation to simultaneously increase production and decrease nitrogen balances, and its impact on crop diversity. Our technological specification extends the by‐production approach by dynamically modelling the impact of the N balance from the previous year on current year crop production. We use a robust order‐m data envelopment analysis to estimate the production frontier, and the Hill‐Shannon index to assess crop diversity before and after optimal cropland reallocation. The application uses Farm Business Survey data from farms in England and Wales between 2015 and 2019. The results show that efficiency gains would have increased crop production by GBP 10.31 per ha and decreased the nitrogen balance by 1.05 kg per ha, when compared with a business‐as‐usual scenario. Reallocation, only focusing on increasing production, would have increased crop production by GBP 83.74 per hectare and reduced the nitrogen balance by 2.01 kg per ha. Reallocation, focusing on increasing production and decreasing the nitrogen balance, would have increased the former by GBP 71.88 per hectare and reduced the latter by 4.99 kg per ha. The median cropland diversity increases by approximately 0.24 species per farm in both reallocation scenarios. Our results suggest that farmers can simultaneously improve economic and environmental performance, which would increase crop diversity. Effective policies should address barriers to diversification and foster management practices that both increase production and decrease nitrogen balances.
Structures, antioxidant, and angiotensin I‐converting enzyme (ACE)‐inhibitory activities of peptides derived from protein hydrolysates of three phenolics‐rich legume genera
Authors: Chang Sam K. C. , Zhang Yan , Pechan Tibor 
Published: February 2025

AbstractLentil, black soybean, and black turtle bean are rich in phenolic antioxidants but belong to different scientific genera with different protein structures. This study's objective was to compare the characteristics of peptides derived from the protein hydrolysates of these legumes. Proteins were isolated, cooked, and subjected to in vitro digestion with pepsin, trypsin, and chymotrypsin. Hydrolyzed peptides were fractionated by ultrafiltration (UF), anion‐exchange chromatography, and gel‐permeation chromatography (GPC). GPC‐eluted peptides of <3 kDa with high angiotensin I‐converting enzyme (ACE)‐inhibitory activities were sequenced. Antioxidant profiles of peptides from the three legumes analyzed by five methods did not follow the same activity patterns associated with the decreases in peptide's molecular size. Among the UF fractions, the <3 kDa fraction had the highest ACE‐inhibition with approximately 45%, 42%, and 39% at 100 µg/mL. Stepwise purifications of the hydrolysates enhanced the ACE‐inhibitory capacity (IC50) by two to nine folds after GPC to approximately 85, 64, and 93 µg/mL for lentil, black soybean, and black turtle bean, respectively. The 210 peptides sequenced in the <3 kDa fractions had chain‐lengths, ranging from 6 to 18 amino acids with lentil having the shortest average length of 7.7 per peptide. Overall, based on chemical analyses, peptides contributed higher antioxidant capacity and ACE‐inhibition than phenolics in legumes. Black turtle bean may need to be heated more prior to consumption to achieve the same digestibility as lentil and soy proteins. Results provided a foundation for making bioactive peptides from the three legumes.Practical ApplicationThree antioxidant‐rich legume genera, lentil, black soybean, and black turtle bean, produced different peptides with different angiotensin I‐converting enzyme (ACE) potencies and different peptide lengths. The results provided a scientific basis for producing peptides from the selected genera for the development of functional foods or dietary supplement with high antioxidant, ACE inhibitory activities, and digestibility.