bluuebunny/crossref_metadata_2025_split huggingface dataset:
Efficacy of Kinesio Taping in Post Operative Sequalae After Surgical Removal of Mandibular Third Molars: a Split Mouth Randomized Control Study
Authors: Patil Sneha , Kambala Rajanikanth K. , Bhola Nitin 
Published: May 2023

Abstract
Background - Surgical removal of impacted lower third molars produces a significant degree of trauma to the soft tissue and bony structures of the oral cavity, which can initiate considerable inflammatory reaction. Consequently, patient experiences pain, swelling, hemorrhage, nerve paraesthesia, limited mouth opening etc. The kinesiologic tape (KT) can help with blood and lymphatic circulation and has shown acceptable outcomes in reducing pain and in managing post-operative muscle spasm.
Methodology – The study was aimed to compare the effects of kinesiologic tape on post operative pain, swelling and trismus following surgical removal of mandibular third molar when compared to control group. 15 patients with bilaterally impacted mandibular third molar were included in this split mouth study. One side was randomly assigned as Group A where patient’s face was subjected to Kinesio tape application post extraction. The other was Group B where Kinesio tape was not applied. After extraction each patient was evaluated in terms of post op pain, swelling and trismus in post-op day 1,2 and 7th.
Results - Pain was found to be significantly less (p =0.042) in group A when compared to group B on the first day. The mean pain was 5.07 in KT group and 6.20 in No KT group on day 2nd. Post OP Swelling was statistically significantly less (p <0.01) in Group A on 2nd & 3rd day. The postoperative mouth opening was more from the 2nd day in group A with mean of 26.07 mm and 20.33 mm in group B (p<0.01).
Conclusion- The kinesiologic taping originates from sports medicine, but can also used therapeutically for reducing post operative sequelae as demonstrated in our study. Kinesiologic tape (KT) enables patients to have a comfortable time post-operatively and helps to regain better quality of life.
Trial Registration – Registered in Clinical Trial Registry - India
Registration number - CTRI/2021/05/033359, registration date – 04/05/2021
Deubiquitinase USP1 enhances CCAAT/enhancer-binding protein beta (C/EBPβ) stability and accelerates adipogenesis and lipid accumulation
Authors: Chun Kyung-Hee , Kim Myung Sup , Baek Jung-Hwan , Lee JinAh 
Published: May 2023

Abstract
Dysregulation of the ubiquitin-proteasome system has been implicated in the pathogenesis of several metabolic disorders, including obesity, diabetes, and non-alcoholic fatty liver disease; however, the mechanisms controlling pathogenic metabolic disorders remain unclear. Transcription factor CCAAT/enhancer binding protein beta (C/EBPβ) regulates adipogenic genes. The study showed that the expression level of C/EBPβ is post-translationally regulated by the deubiquitinase ubiquitin-specific protease 1 (USP1) and that USP1 expression is remarkably upregulated during adipocyte differentiation and in the adipose tissue of mice fed a high-fat diet (HFD). We found that USP1 directly interacts with C/EBPβ. Knock-down of USP1 decreased C/EBPβ protein stability and increased its ubiquitination. Overexpression of USP1 regulates its protein stability and ubiquitination, whereas catalytic mutant of USP1 had no effect on them. It suggests that USP1 directly deubiquitinases C/EBPβ and increases the protein expression, leading to adipogenesis and lipid accumulation. Notably, the USP1-specific inhibitor ML323—originally developed to sensitize cancer cells to DNA-damaging agents—decreased adipocyte differentiation and lipid accumulation in 3T3-L1 cells without cytotoxicity. Oral gavage of ML323 was administered to HFD-fed mice, which showed weight loss and improvement in insulin and glucose sensitivity. Both fat mass and adipocyte size in white adipose tissues were significantly reduced by ML323 treatment, which also reduced expression of genes involved in adipogenesis and inflammatory responses. ML323 also reduced lipid accumulation, hepatic triglycerides, free fatty acids, and macrophage infiltration in the livers of HFD-fed mice. Taken together, we suggest that USP1 plays an important role in adipogenesis by regulating C/EBPβ ubiquitination, and USP1-specific inhibitor ML323 is a potential treatment option and further study by ML323 is needed for clinical application for metabolic disorders.
Analysis of influencing factors of revascularization after percutaneous coronary intervention in patients with coronary heart disease and construction of nomogram model
Authors: Zhou Xuelian , Yu Hongwei , Miao Xiao , Wang Shaomin , Li Xiaobo , Zhu Liqun , Cao Songmei 
Published: May 2023

Abstract
Objective To analyze the influencing factors of revascularization in patients with coronary heart disease after PCI, and to construct a Nomo diagram model of revascularization in patients with coronary heart disease after PCI and conduct internal verification.
Methods Patients with coronary heart disease who underwent PCI in the National Key Specialty Cardiology Department of a tertiary and first-class hospital in Zhenjiang City from January 2015 to November 2015 were retrospectively collected as the research subjects. Multivariate Logistic regression was used to analyze the influencing factors of repeat revascularization after PCI in patients with coronary heart disease, and a nomogram model was established to predict the risk of repeat revascularization after PCI in patients with coronary heart disease. The predictive performance of the model was internally validated using ROC curves.
Results Multivariate analysis showed that poor daily life behaviors [OR=4.208, 95%CI(1.828, 9.686)], high risk of coronary heart disease specific index [OR=3.308, 95%CI(1.157, 9.453)], high fat Hyperemia [OR=7.262, 95%CI(3.236, 16.296)], multivessel disease [OR=2.691, 95%CI(1.061, 6.822); OR=6.068, 95%CI(2.467, 14.925)] was coronary heart disease The independent risk factors of revascularization in patients (P<0.05) were included in the model. Model internal validation AUC value was 0.830 [95%CI (0.782, 0.870), P<0.05], Youden index was 0.5530, specificity was 85.48%, sensitivity was 69.81%, and cut-off value was 122.39 points.
Conclusion The main influencing factors of poor daily living behavior, high risk of coronary heart disease-specific index, hyperlipidemia and multivessel disease, the nomogram model established based on the above factors has good predictive performance.
Clinically used broad-spectrum antibiotics compromise inflammatory monocyte-dependent antibacterial defense in the lung
Authors: Opitz Bastian , Dörner Patrick , Anandakumar Harithaa , Röwekamp Ivo , Milek Miha , Vernengo Facundo Fiocca , Pascual-Leone Belen Millet , Krzanowski Marta , Igbokwe Vanessa , Loeber Ulrike , Holstein Markus , Heinz Gitta , Mashreghi Mir-Farzin , Schulte Leon , Klatt Ann-Brit , Caesar Sandra , Wienhold Sandra-Maria , Offermanns Stefan , Mack Matthias , Witzenrath Martin , Beule Dieter , Forslund Sofia , Wilck Nicola , Bartolomaeus Hendrik , Heimesaat Markus 
Published: May 2023

Abstract
Hospital-acquired pneumonia (HAP) is associated with high mortality and costs, and frequently caused by multidrug-resistant (MDR) bacteria. Although prior antimicrobial therapy is a major risk factor for HAP, the underlying mechanism remains incompletely understood. Here, we demonstrate that antibiotic therapy in hospitalized patients is associated with decreased diversity of the gut microbiome and depletion of short-chain fatty acid (SCFA) producers. Mice transplanted with patient gut microbiota reveal that these antibiotic-induced microbiota perturbations compromise pulmonary defense against MDR Klebsiella pneumoniae by compromising free fatty acid receptor (FFAR)2/3-controlled antibacterial activity of inflammatory monocytes (IMs). Collectively, we characterize how clinically relevant antibiotics affect antimicrobial defense in the context of human microbiota, and reveal a critical impairment of IMs. Our study provides additional arguments for the rational use of antibiotics and offers mechanistic insights for the development of novel prophylactic strategies to protect high-risk patients from HAP.
Structural insights into MIC2 recognition by MIC2-associated protein
Authors: Zhang Su , Wang Fangfang , Liu dongsheng , Ding Wei , Springer timothy , Zhang Dujuan , Song Gaojie 
Published: May 2023

Abstract
Microneme protein 2 (MIC2) and MIC2-associated protein (M2AP) play crucial roles in the gliding motility and host cell invasion of Toxoplasma gondii. Complex formation between MIC2 and M2AP is required for maturation and transport from the microneme to the parasite surface. Previous studies showed that M2AP associates with the 6th TSR domain of MIC2 (TSR6), but the detailed interaction remains unclear. In this study, we determined crystal structures of MA2P alone and in complex with TSR6. TSR domains have an unusually thin, long structure with a layer of intercalated residues on one side. The non-layered side of TSR6 with hotspot residue His-620 at the center binds to M2AP. Remarkably, we discovered that TSR6 residue Y602 is dynamic; it equilibrates between being part of the layer (the layered state) and in a flipped-out state in the absence of M2AP. However, when bound to M2AP, Y602 shifts to the flipped-out state. Our findings provide insights into the association and stabilization of MIC2-M2AP complex, and may be used to develop new therapies to prevent infections caused by this parasite.
Clinical study of the early use of ivabradine in patients with acute myocardial infarction after percutaneous coronary intervention
Authors: He Ruiqing , Li Lingyan , Han Chao , An Wen , Liu Zongjun , Gao Junqing 
Published: May 2023

AbstractObjective To investigate the efficacy of early use of ivabradine in patients with acute myocardial infarction (AMI) after percutaneous coronary intervention (PCI). Methods Eighty patients with AMI were randomly divided into the ivabradine group and the control group. The ivabradine group was treated with ivabradine combined with metoprolol after PCI, while the control group was treated with metoprolol only. Both groups were treated continuously for 1 year. Echocardiography-derived parameters, heart rate, cardiopulmonary exercise testing (CPET) data, major adverse cardiac events (MACE) and myocardial markers were analyzed. Results The echocardiography-derived left ventricular ejection fraction was significantly higher in the ivabradine group than in the control group at 1 week, 3 months and 1 year after PCI. The heart rate of the ivabradine group was significantly lower than that of the control group at 1 week after PCI. The VO2max, metabolic equivalents, anaerobic threshold heart rate, peak heart rate, and heart rate recovery at 8 minutes of the ivabradine group were significantly higher than those of the control group at 1 year after PCI. The one-year total incidence of major adverse cardiac events was 10% in the ivabradine group, which was significantly lower than the 37.5% in the control group. The B-type natriuretic peptide of the ivabradine group was significantly lower than that of the control group on Day 2 and Day 3 after PCI. The troponin I level of the ivabradine group was significantly lower than that of the control group on Day 5 after PCI. Conclusion Early use of ivabradine in patients with AMI after PCI can achieve effective heart rate control, reduce myocardial injury, improve cardiac function and exercise tolerance, and reduce the incidence of major adverse cardiac events.
Discrimination of Camellia cultivars using iD-NA analysis
Authors: Kikkawa Hitomi S. , Sato Mitsuhiko P. , Matsuo Ayumi , Sasaki Takanori , Suyama Yoshihisa , Tsuge Kouichiro 
Published: May 2023

Abstract
Recently, many new cultivars are taken abroad illegally, which is now considered an international issue. Botanical evidence found at a crime scene provides valuable information about the origin of the sample. However, botanical resources for forensic evidence remain underutilized because molecular markers, such as microsatellites, are not available without a limited set of species. Multiplexed ISSR genotyping by sequencing (MIG-seq) has been used to determine a number of genome-wide genetic markers and can be applied for every plant, including species that lack genetic information. Camellia cultivars are popular worldwide and are often planted in many gardens and bred to make new cultivars. In this study, we aimed to analyze Camellia cultivars/species through MIG-seq. Through this, MIG-seq could discriminate similar samples, such as bud mutations and closely related samples which could not be distinguished based on morphological features. This discrimination was consistent with that of a previous study which classified cultivars by short tandem repeat (STR) markers, indicating that MIG-seq has the same or higher discrimination ability as STR markers. Furthermore, based on our results, unknown phylogenetic relationships were observed. Since MIG-seq can be applied to unlimited species and low-quality DNA, it may be useful for various scientific fields.
Tree communities and functional traits determine herbivore compositional turnover
Authors: Wang Ming-Qiang , Wen Zhixin , Ke Jinzhao , Chesters Douglas , Li Yi , Chen Jing-Ting , Luo Arong , Shi Xiaoyu , Zhou Qing-Song , Liu Xiao-Juan , Ma Keping , Bruelheide Helge , Schuldt Andreas , Zhu Chao-Dong 
Published: May 2023

Abstract
There are many factors known to drive species turnover although the mechanisms by which these operate are less clear. Based on comprehensive datasets we used zeta diversity and multi-site generalized dissimilarity modelling to investigate the pattern and determinants of species turnover for Lepidoptera herbivores. We found that the average number of shared herbivore species declined sharply with an increasing number of plots, with complete changes in caterpillar species composition observed even at fine spatial scales. Plant community characteristics rather than abiotic factors were found to play key roles in driving caterpillar compositional turnover, although these effects varied with increasing plots due to the varying contributions of rare and common species to compositional turnover. Our study reveals details of the impact of phylogeny- and trait- mediated processes of trees on herbivore compositional turnover, which has implications for forest management and conservation and potential avenues for maintenance of heterogeneity in herbivore communities.
Abnormal grey matter structural changes in patients with end-stage kidney disease and mild cognitive impairment: correlations with clinical features
Authors: Yuan Huijie , Luo Zhaoyao , Gu Wen , Ma Shaohui , Li Guangyu , Ding Dun , Ma Xueying , Li Peng , Yang Jing , Xu Xiaoling , Mu Junya , Zhang Ming 
Published: May 2023

Abstract
End-stage kidney disease and mild cognitive impairment (ESKD-MCI) affect the quality of life and long-term treatment outcomes of patients affected by these diseases. Clarifying the morphological changes from brain injuries in ESKD-MCI and their relationship with clinical features is helpful for the early identification and intervention of MCI, before it progresses irreversible dementia. This study gathered data from 23 patients with ESKD-MCI, 24 patients with ESKD and non-cognitive impairment (NCI), and 27 health controls (HCs). Structural magnetic resonance studies, cognitive assessments, and general clinical data were collected from all of the participants. Voxel-based morphometry analysis was performed to compare grey matter (GM) volume differences between the groups. The patients’ GM maps and clinical features were subjected to univariate regression, to check for possible correlations. Patients with ESKD-MCI displayed significantly more impairments in multiple cognitive domains including global cognition, visuospatial and executive function, and memory, as compared with patients with ESKD-NCI. Using a more liberal threshold (P < 0.001, uncorrected), we found that compared to patients with ESKD-NCI, patients with ESKD-MCI exhibited clusters of regions with lower GM volumes, including the right hippocampus (HIP), parahippocampal gyrus (PHG), Rolandic operculum, and supramarginal gyrus. The volumes of the right HIP and PHG were negatively correlated with serum calcium levels. ESKD-MCI was associated with a subtle volume reduction of GM in several brain areas known to be involved in memory, language, and auditory information processing. We speculate that these slight morphometric impairments may be associated with disturbed calcium metabolism.
A Case of Cerebral Cavernous Malformations Misdiagnosed as Multiple Myeloma
Authors: Zhao Yinan , Gu Yan 
Published: May 2023

Abstract
Cerebral cavernous malformations (CCMs) are angiographically occult cerebrovascular malformations (AOVM) of the central nervous system. They are composed of a sponge-like abnormal vascular mass with numerous thin-walled vessels. CCM incidence rate is approximately 7% of cerebrovascular malformations. The clinical manifestations mainly include seizures, bleeding, headache, etc., with seizures being the most common first symptom, and some patients may be without clinical manifestations. MRI has been the specific diagnostic method for CCMs. This article reports the case of a patient initially diagnosed with multiple myeloma in the left temporal lobe at our hospital in September 2020. Later, the patient was confirmed to have CCMs through surgery and pathology. The main symptom was a sudden onset of cognitive dysfunction, and the patient was discharged after receiving symptomatic and surgical interventions.
Design and fabrication of sculptured thin films for application in control of cancer and other cells
Authors: Shayegan Fahimeh Farid , Savaloni Hadi , Zali Hakimeh , Niknam Zahra 
Published: May 2023

Abstract
Given the importance of cell colonization for use in tissue engineering and for studying the inhibition of cancer cells, the use of bio material sculptured nano-structures mimicking the structure of the extracellular matrix offers a unique perspective. In this study, Mn and ZnS sculptured nano-structured fabricated using oblique (OAD) and glancing (GLAD) angle vapor deposition methods for investigation of cell behavior. Different characteristics of the produced sculptured thin films of Mn and ZnS materials in form of helical square and 3- and 5-fold helical star shapes were obtained from XRD, FESEM, and AFM analyses. Measurement of contact angle classified the produced samples in two groups of hydrophilic and hydrophobic samples, which provided the opportunity to investigate their function when cultured with normal and cancerous cells. Results showed that the hydrophilic surfaces increased the proliferation of both normal and cancer cells, albeit with different rates. On the contrary, the hydrophobic surfaces prevented the proliferation of cells and their expansion and cells undergo cell death or apoptosis. The specific morphology of the hydrophilic sculptured samples makes it possible to send proliferation signals for tissue engineering purposes, and in hydrophobic samples, to send apoptotic signals to inhibit cancer cells.
Lipids-related Domestication Accounts for the Extreme Cold-sensitivity of Semiwild and Tropic Xishuangbanna cucumber (Cucumis sativus L. var. xishuangbannanesis)
Authors: Zhang Rui-Jing , Liu Bin , Song Shan-Shan , Salah Radwa , Song Chang-Jiang , Zhen Xiao-Fang , Xia Shi-Wei , Hao Qian , Liu Yan-Jun , Li Yu , Li Huan-Xiu , Lai Yun-Song 
Published: May 2023

Abstract
Xishuangbanna (XIS) cucumber (Cucumis sativus L. var. xishuangbannanesis) is a semiwild variety originated from low latitude tropic area, and therefore show extreme cold sensitivity and heat tolerance. Here, we mapped the quantitative trait loci (QTLs) that control cold-sensitivity and heat-tolerance of XIS cucumber seedlings. By bulked-segregant analysis (BSA), we identified three QTLs (HTT1.1, HTT3.1 and HTT3.2, in a total length of 11.98 Mb) for heat tolerance and two QTLs (LTT6.1 and LTT6.2, in a total length of 8.74 Mb) for cold sensitivity. The QTL LTT6.1 was then narrowed down to a length of 641 kb. Based on structural variants (SVs) and single nucleotide polymorphism (SNP), we found the LTT6.1 is covered by a high divergent region including a 50-kb deletion in XIS49 genome, which affect the gene structure of lipase ABHD6 (Csa_6G032560). Accordingly, there is a very big difference in lipid composition but not other osmoprotectants like free amino acids and fatty acids between XIS49 and cultivated cucumber CL. Moreover, we calculated the composite likelihood ratio (CLR) and identified selective sweeps from 115 resequencing data, and found that lipid- and fatty acid-related processes is a major aspect of the domestication of XIS group cucumber. LTT6.1 is such a special region positioned nearby lipid-related selective sweeps. These studies above suggested that lipids-related domestication of XIS cucumbers should account for their extreme cold sensitivity.
Immunological and Biochemical Biomarker Alterations Among SARS-COV-2 Patients with Varying Disease Phenotypes in Uganda
Authors: Kato Charles Drago , Nsubuga Julius , Niyonzima Nixon , Kitibwa Annah , Matovu Enock , Othieno Emmanuel , Ssebugere Patrick , Tumwine Amanda Agnes , Namayanja Monica 
Published: May 2023

Abstract
Every novel infection requires an assessment of the host response coupled with identification of unique biomarkers for predicting disease pathogenesis, treatment targets and diagnostic utility. Studies have exposed dysregulated inflammatory response induced by the novel severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) as significant predictor or cause of disease severity/prognosis and death. This study evaluated inflammatory biomarkers induced by SARS-CoV-2 in plasma of patients with varying disease phenotypes and healthy controls with prognostic or therapeutic potential. We stratified SARS-CoV-2 plasma samples based on disease status (asymptomatic, mild, severe, and healthy controls), as diagnosed by RT-PCR SARS-CoV-2. We used a solid phase sandwich and competitive Enzyme-Linked Immunosorbent Assay (ELISA) to measure levels of panels of immunological (IFN-γ, TNF-α, IL-6, and IL-10) and biochemical markers (Ferritin, Procalcitonin, C-Reactive Protein, Angiotensin II, Homocysteine, and D-dimer). Biomarker levels were compared across SARS-CoV-2 disease stratification. Plasma IFN-γ, TNF-α, IL-6, and IL-10 levels were significantly (P < 0.05) elevated in the severe SARS-CoV-2 patients as compared to mild, asymptomatic, and healthy controls. Ferritin, Homocysteine, and D-dimer plasma levels were significantly elevated in severe cases over asymptomatic and healthy controls. Plasma C-Reactive Protein and Angiotensin II levels were significantly (P < 0.05) higher in mild than severe cases and healthy controls. Plasma Procalcitonin levels were significantly higher in asymptomatic than in mild, severe cases and healthy controls. Our study demonstrates the role of host inflammatory biomarkers in modulating the pathogenesis of COVID-19. The study proposes a number of potential biomarkers that could be explored as SARS-CoV-2 treatment targets and possible prognostic predictors for a severe outcome. The comprehensive analysis of prognostic biomarkers may contribute to the evidence-based management of COVID-19 patients.
Fusion of Probabilistic Projections of Sea Level
Authors: Grandey Benjamin S. , Dauwels Justin , Horton Benjamin P. , Chew Lock Yue 
Published: May 2023

Abstract

To combine the complementary strengths of model-based and expert-based projections of sea level, we propose a fusion. The fusion follows the model-based probabilistic projection in the centre of the distribution and the expert-based probabilistic projection in the upper tail. The fusion can be interpreted intuitively as a single probabilistic projection and meets the needs of diverse stakeholders.