bluuebunny/crossref_metadata_2025_split huggingface dataset:
Clinical Evaluation of Predisposing Factors of Hypoglycaemia in Diabetic Patients
Authors: Rahman Mohammad Motiur , Talukder Suzauddin , Baker Issa Muhammad , Das Anupam , Baset Md Abdul , Najnin Farjana 
Published: January 2023

Background: Hypoglycemia is a common medical problem in diabetic patients, and the potential range of morbidity and mortality in diabetic patients exposed to hypoglycemia is significant. The study was conducted to evaluate the predisposing factors of hypoglycemia in diabetic populations.
Methods: This is a descriptive cross-sectional study carried out in the indoor and outdoor patient department of BIRDEM hospital, Dhaka, Bangladesh, from July 2009 to December 2009 after ethical approval in conformity with the revised 1964 Helsinki declaration. Following informed written consent, a total of 50 diabetic patients having hypoglycemia were finally included. Data analysis was done using SPSS 17.
Results: In the study population, significant members of hypoglycemic episodes were found in middle age (31-70) insulin-treated patients and poor diabetic education and in those who had other concomitant diseases. Poor dietary compliance (Reduced meal intake in 50% of cases and missed meal in 34% of cases) and lack of proper diabetic education about early recognition and treatment of hypoglycemia also appeared as important contributory factors. Noncompliance with regular exercise (40%) and unaccustomed exercise (4%) contribute to hypoglycemia. A significant number of hypoglycemic patients have chronic renal insufficiency (68%).
Conclusion: Hypoglycemia is common in the diabetic population who have poor knowledge and practice about diet, drug exercise technique of, use of insulin devices, and have an underlying systemic illness. So more rational education system and appropriate management of systemic diseases regarding the prevention and treatment of hypoglycemia is demanded by our diabetic patients.
TAJ 2022; 35: No-2: 143-149
Glomerulonephritis Following Russell’s Viper Bite – 1st Case Report in Bangladesh
Authors: Rahman Abu Shahin Mohammed Mahbubur , Rahman M Siddiqur , Haque Md Zahirul , Rahman Md Khalilur , Amin M Robed , Faiz MA 
Published: January 2023

Russell’s viper bite causing coagulopathy, hypotension, DIC, rhabdomyolysis and acute kidney injury. Nephropathy usually is caused by RV having hemotoxic or myotoxic venom components. Multiple mechanism causes renal pathologic changes including tubular necrosis, cortical necrosis, interstitial nephritis, and glomerulonephritis. But above all glomerulonephritis is very rare. We report a case of Russell’s viper bite who died of renal failure due to focal segmental proliferative glomerulonephritis admitted int the Rajshahi Medical College Hospital, Rajshahi, Bangladesh.
TAJ 2022; 35: No-2: 169-172
La praxis metodológica del Pensamiento Complejo fortalece el desarrollo integral del Sector cooperativo-solidario agropecuario colombiano
Authors: Ramírez-Miranda Jorge 
Published: January 2023

Este artículo tiene como propósito presentar una síntesis de los resultados del tema-problema de investigación de la tesis doctoral “Participación y perspectivas del Sector cooperativo-solidario agropecuario colombiano (Scsacol) en la Economía Solidaria y el sector agropecuario nacional (2006-2019): Propuesta pedagógico-didáctica desde la praxis del Pensamiento Complejo articulada a la Economía Solidaria Colombiana”. El estudio requería la elección de un método de investigación pertinente; para el efecto, el autor preliminarmente examinó cuál era el método de investigación más adecuado y a su juicio, eligió el Pensamiento Complejo por considerarlo el más conveniente a ser aplicado en los diferentes tópicos relacionados con el desarrollo del proyecto y a explicar las razones de su elección.

Una vez definido el método del estudio, procedió a aplicarlo y trabajar en el desarrollo de sus objetivos: 1) determinar las causas, desarrollo y efectos socioeconómicos y políticos por los cuales se presenta la baja participación de éste sector en la economía nacional y solidaria y el sector agropecuario colombiano; 2) explicar las razones por las cuales se eligió el Pensamiento Complejo en el estudio y su articulación con los principios, valores y emprendimiento del sector solidario colombiano y 3) Proponer al (Scsacol) su fortalecimiento integral desde la praxis pedagógico-didáctica del Pensamiento Complejo articulada a los principios, valores y emprendimiento solidario planteados por el sector de la Economía solidaria colombiana (ESC).

La información para este estudio se obtuvo principalmente del Estado colombiano, de investigadora(es) de la (ESC), del sector agropecuario, de organizaciones de la sociedad civil nacional e internacional del paradigma moriniano y de estudiosos del tema-problema investigativo; información que posibilitó realizar los objetivos del estudio y de la mencionada propuesta pedagógica. A través del desarrollo del proyecto se comprobó que la elección y aplicación metodológica del Pensamiento Complejo resultó ser la más pertinente.
Boost dynamic protocols for producing mammalian biopharmaceuticals with intensified DoE—a practical guide to analyses with OLS and hybrid modeling
Authors: Nold V. , Junghans L. , Bayer B. , Bisgen L. , Duerkop M. , Drerup R. , Presser B. , Schwab T. , Bluhmki E. , Wieschalka S. , Knapp B. 
Published: January 2023

Introduction: For the implementation of robust bioprocesses, understanding of temporal cell behavior with respect to relevant inputs is crucial. Intensified Design of Experiments (iDoE) is an efficient tool to assess the joint influence of input parameters by including intra-experimental changes.Methods: We applied iDoE to the production phase of a monoclonal antibody in a mammalian bioprocess. The multidimensional design space spanned by temperature, dissolved oxygen (DO), timing of change, and growth category was investigated in 12 cultivations. We built ordinary least squares (OLS) and hybrid models (HM) on the iDoE-data, validated them with classical DoE (cDoE)-derived data, and used the models as in silico representation for process optimization.Results: If the complexity of interactions between changing setpoints of inputs is sufficiently captured during planning and modeling, iDoE proved to be valid for characterizing the mammalian biopharmaceutical production phase. For local behavior and flexible composition of optimization goals, OLS regressions can easily be implemented. To predict global and interconnected dynamics while incorporating mass balances, HM holds potential.Discussion: iDoE will boost protocols that optimize inputs for different bioprocess phases. The described key aspects of OLS- and HM-based analyses of iDoE-data shall guide future applications during manufacturing.
Roles of vaginal flora in human papillomavirus infection, virus persistence and clearance
Authors: Zeng Mi , Li Xin , Jiao Xiaoyang , Cai Xiaochun , Yao Fen , Xu Shaomin , Huang Xiaoshan , Zhang Qiaoxin , Chen Jianqiang 
Published: January 2023

Vaginal flora plays a vital role in human papillomavirus (HPV) infection and progression to cancer. To reveal a role of the vaginal flora in HPV persistence and clearance, 90 patients with HPV infection and 45 healthy individuals were enrolled in this study and their vaginal flora were analyzed. Women with HPV infection were treated with Lactobacillus in the vaginal environment as a supplement to interferon therapy. Our results indicated that patients with high risk HPV (Hr-HPV) 16/18 infection had a significantly higher alpha diversity compared with the healthy control (p &lt; 0.01), while there was no significant difference between the non-Hr-HPV16/18 group and the controls (p &gt; 0.05). Patients with multiple HPV infection had insignificantly higher alpha diversity compared with single HPV infection (p &gt; 0.05). The vaginal flora of patients with HPV infection exhibited different compositions when compared to the healthy controls. The dominant bacteria with the highest prevalence in HPV-positive group were Lactobacillus iners (n = 49, 54.44%), and the top 3 dominant bacteria in the HPV-persistent group were Lactobacillus iners (n = 34, 53.13%), Sneathia amnii (n = 9, 14.06%), and Lactobacillus delbrueckii (n = 3, 4.69%). Patients with HPV clearance had significantly lower alpha diversity, and the flora pattern was also different between groups displaying HPV clearance vs. persistence. The patients with persistent HPV infection had significantly higher levels of Bacteroidaceae, Erysipelotrichaceae, Helicobacteraceae, Neisseriaceae, Streptococcaceae (family level), and Fusobacterium, Bacteroides, Neisseria, and Helicobacter (genus level) than patients who had cleared HPV (p &lt; 0.05).ImportanceOur study revealed differences in vaginal flora patterns are associated with HPV persistence and its clearance. Interferon plus probiotics can greatly improve virus clearance in some patients. Distinguishing bacterial features associated with HPV clearance in patients would be helpful for early intervention and reverse persistent infection.
Parasitoid-mediated horizontal transmission of Rickettsia between whiteflies
Authors: Liu Yuan , He Zi-Qi , Wen Qin , Peng Jing , Zhou Yu-Tong , Mandour Nasser , McKenzie Cindy L. , Ahmed Muhammad Z. , Qiu Bao-Li 
Published: January 2023

Intracellular bacterial endosymbionts of arthropods are mainly transmitted vertically from mother to offspring, but phylogenetically distant insect hosts often harbor identical endosymbionts, indicating that horizontal transmission from one species to another occurs in nature. Here, we investigated the parasitoid Encarsia formosa-mediated horizontal transmission of the endosymbiont Rickettsia between different populations of whitefly Bemisia tabaci MEAM1. Rickettsia was successfully transmitted from the positive MEAM1 nymphs (R+) into E. formosa and retained at least for 48 h in E. formosa adults. Fluorescence in situ hybridization (FISH) visualization results revealed that the ovipositors, mouthparts, and digestive tract of parasitoid adults get contaminated with Rickettsia. Random non-lethal probing of Rickettisia-negative (R−) MEAM1 nymphs by these Rickettsia-carrying E. formosa resulted in newly infected MEAM1 nymphs, and the vertical transmission of Rickettsia within the recipient females can remain at least up to F3 generation. Further phylogenetic analyses revealed that Rickettsia had high fidelity during the horizontal transmission in whiteflies and parasitoids. Our findings may help to explain why Rickettsia bacteria are so abundant in arthropods and suggest that, in some insect species that shared the same parasitoids, Rickettsia may be maintained in populations by horizontal transmission.
Association of body mass index trajectory and hypertension risk: A systematic review of cohort studies and network meta-analysis of 89,094 participants
Authors: Tan Ling , Long Lin-zi , Ma Xiao-chang , Yang Wen-wen , Liao Fei-fei , Peng Yu-xuan , Lu Jie-ming , Shen A-ling , An Dong-qing , Qu Hua , Fu Chang-geng 
Published: January 2023

IntroductionBody mass index (BMI) trajectories, such as non-linear time trends and nonlinear changes in BMI with age, can provide information on the underlying temporal health patterns. The relationship between BMI trajectories and the risk of hypertension remains controversial.MethodsPubMed, Embase, Cochrane, Scopus, and Web of Science databases were searched from their inception to January 31, 2022. We categorized BMI trajectories as “Stable high,” “table normal,” “Stable low,” “Fluctuated (sharp increase),” and “Fluctuated (elevated-decrease).” The main outcome was the relative risk for the prevalence of hypertension in the different BMI trajectories. Potential sources of heterogeneity were examined using meta-regression and subgroup analysis. A publication bias test and Grading of Recommendations Assessment, Development and Evaluation (GRADE) approach were also used.ResultsThe 18 cohort studies included 89,094 participants. Compared with the “Stable normal” trajectory, “Stable high,” “Fluctuated (sharp increase),” and “Fluctuated (elevated-decrease)” trajectories were associated with an increased relative risk of hypertension: [RR (95% CI)]: 1.80 (1.29 2.50), p &lt; 0.001; 1.53 (1.27 1.83), p &lt; 0.001; 1.30 (1.24 1.37), p = 0.001, respectively. The “Stable low” trajectory was associated with a reduced risk of hypertension [0.83 (0.79 0.83), p &lt; 0.001]. The “Stable high” trajectory (surface under the cumulative ranking curve = 88.1%) had the highest probability of developing hypertension in the population. The certainty of the evidence for direct comparisons of the incidence of hypertension between various BMI trajectories was generally very low.ConclusionOur findings suggested that “Stable high,” “Fluctuated (sharp increase),” and “Fluctuated (elevated-decrease)” trajectories were associated with an increased relative risk of hypertension, with the “Stable high” trajectory most likely associated with hypertension.Systematic review registration[https://www.crd.york.ac.uk/prospero/display_record.php?RecordID=308575], identifier [CRD42022308575].
Intra-oceanic subduction in the southeastern Paleo-Asian ocean: Evidence from early Permian gabbro of the Diyanmiao ophiolite in central Inner Mongolia, North China
Authors: Zhang Xiawei , Li Yingjie , Wang Genhou , Wang Shuai , Kong Xingrui , Wang Xiaodong , Liu Zhibin 
Published: January 2023

Investigation of intra-oceanic subduction can improve our understanding of plate tectonic processes and the history of continental growth. Evidence for intra-oceanic subduction in the Paleo-Asian Ocean has recently become an important focus of research, including the Diyanmiao ophiolite in central Inner Mongolia, North China. Here, we report a newly discovered occurrence of early Permian gabbro in the Diyanmiao ophiolite zone. The gabbro yields a weighted mean zircon U–Pb age of 294.4 ± 2.2 Ma. The gabbro samples are characterized by moderate SiO2 (47.32–50.51 wt%), low TiO2 (0.26–0.54 wt%) and K2O (0.04–0.75 wt%), and high Na2O (1.84–4.52 wt%) contents, high Na2O/K2O ratios (2.92–58.29), and depleted chondrite-normalized light rare Earth element patterns that are similar to N-MORB. The gabbros show slightly lower contents of high-field-strength elements (e.g., Nb, Ta, and Ti) and slightly higher contents of large-ion lithophile elements (e.g., K, Rb, Ba, and U) relative to N-MORB. In addition, the gabbros show high εNd (t) values (8.0–9.8) that are similar to those of forearc basalt in the Diyanmiao ophiolite and N-MORB. Integrating these new data with available results for ophiolite and arc-magmatic rocks of central Inner Mongolia, we propose that the studied gabbro was formed during the initial stage of intra-oceanic subduction and that the Paleo-Asian Ocean was still in a subduction setting during the early Permian.
Three-dimensional characterization and quantitative research of Malan loess microstructure under seismic loading
Authors: Wei Tingting , Wu Zhijian , Chen Yanping , Luo Liang 
Published: January 2023

The deformation and failure of loess in areas of high seismic intensity are closely related to the dynamic vulnerability, which is primarily controlled by the loess microstructure. This study performed a series of dynamic triaxial tests and microstructure tests on intact loess to track and quantitatively characterize the evolution of the three-dimensional microstructure during deformation. The microstructural observations were performed using micro-CT on the samples after varying vibration times. The microstructure parameters (including pore radius, elongation, orientation, coordination number, pore throat area, and channel length) were obtained using a reconstructed pore network model. The results of this study demonstrated that the loess seismic subsidence originated from both compositional and microstructural characteristics. The intact loess had a loose structure with high porosity and limited cementation. Upon cyclic loading, cementation and contact breakdown led to the failure of the loess structure, followed by particle rearrangement. With increasing vibration times, the spaced and inter-aggregate pores became intra-aggregate pores, the pore throat size tended to decrease while the pore number tended to increase, the connectivity tended to weaken, and the shape tended to be long and flat. Pores &gt;28 μm mainly provided spatial conditions for collapse deformation under seismic load. In addition, under the ultimate loading, large-volume cracks occurred inside the sample. The findings of this study provide further insights into loess seismic subsidence from the perspective of three-dimensional microstructures and a research basis for analyzing the stability of loess in relation to construction projects by combining finite and discrete elements.
Student experience scale: Factor analysis in higher education institutions
Authors: Amado Mateus Marelby , Guzmán Rincón Alfredo , Cuero Acosta Yonni Angel 
Published: January 2023

IntroductionIn the context of higher education, the student experience (SX) has an important impact on student satisfaction and the perceived value of the training service received. For these reasons, higher education institutions (HEIs), which are focused on student-centred learning, need to know more about the experience that results from student interaction with the service and support systems that meet their needs in these institutions. However, despite the progress made in the analysis of SX, further research is still needed to know how to measure and manage it, and for this, it is essential to know the student’s perceptions.MethodsFor this reason, an SX scale was selected to have a suitable tool for experience management. Thus, the psychometric properties of the selected scale were evaluated in undergraduate students at two Colombian universities. The validation process consisted of an adaptation of the scale, a content validity analysis, an exploratory factor analysis and a confirmatory factor analysis.ResultsThe results were satisfactory showing the reliability and validity of the student experience scale in the context of universities.DiscussionTherefore, its cross-cultural goodness is confirmed in the student segment and it is suggested to perform this same process for other university audiences.
Genetic polymorphisms of Ca2+ transport proteins and molecular chaperones in mitochondria-associated endoplasmic reticulum membrane and non-alcoholic fatty liver disease
Authors: Tang Zongzhe , Ding Yajie , Zhang Ru , Zhang Mengting , Guan Qing , Zhang Liuxin , Wang Hongliang , Chen Yue , Jiang Rong , Zhang Wei , Wang Jie 
Published: January 2023

BackgroundNon-alcoholic fatty liver disease (NAFLD) is recognized to be closely associated with endoplasmic reticulum stress and mitochondrial dysfunction, while previous studies have emphasized the important role of calcium homeostasis from the mitochondria-associated endoplasmic reticulum membrane (MAM) in the endoplasmic reticulum and mitochondria. This article will assess the association between genetic polymorphisms of Ca2+ transport proteins and molecular chaperones in MAM and NAFLD risk.MethodsA case-control study was conducted in a community of Nanjing, China during April to December 2020. 2701 subjects were enrolled and genotyped for 6 genetic variants in HSPA5 and ITPR2 genes. Logistic regression analysis was used to assess impact of these variants on NAFLD risk.ResultsAfter adjusting for age, gender, total cholesterol and glucose, we identified that HSPA5 rs12009 variant genotypes (recessive model: OR= 0.801, 95% CI= 0.652-0.986, P= 0.036), rs430397 variant genotypes (recessive model: OR= 0.546, 95% CI= 0.314-0.950, P= 0.032), and ITPR2 rs11048570 variant genotypes (recessive model: OR= 0.673, 95% CI= 0.453-0.999, P= 0.049) were associated with a reduced risk of NAFLD. Multivariate stepwise regression analysis indicated that gender, glucose, body mass index, triglycerides and favorable alleles were independent influencers of NAFLD (all P&lt; 0.05). The area under the receiver operating characteristic curve was 0.764 (95% CI= 0.745-0.783, P&lt; 0.001).ConclusionThe variant genotypes of Ca2+ transport-associated genes HSPA5 (rs12009 and rs430397) and ITPR2 (rs11048570) might contribute to the reduction of the NAFLD risk in Chinese Han population, which can provide new insight into NAFLD pathogenesis.
Characterization of the microenvironment of diabetic foot ulcers and potential drug identification based on scRNA-seq
Authors: Li Yao , Ju Shuai , Li Xiaoyan , Li Wenqiang , Zhou Siyuan , Wang Guili , Cai Yunmin , Dong Zhihui 
Published: January 2023

BackgroundDiabetes foot ulcers (DFUs) are a type of foot infection, ulcer, and/or deep tissue destruction caused by neuropathy and vascular disease in the distal extremities of diabetic patients. Its pathogenesis and its microenvironment are not entirely understood.MethodsInitially, the GSE165816 data set from the GEO database was utilized for single cell analysis to reveal the microenvironment and functional status of DFUs. The GSE199939 RNA-seq data set was utilized for external validation. On the basis of the logistic regression machine learning algorithm (OCLR), pseudo time series analysis, dryness index analysis, and drug target gene analysis were then performed. By constructing drug-gene and gene-gene networks, we can locate the most recent DFUs treatments. Finally, immunofluorescence technology was used to detect the cell-related markers of the DFUs microenvironment, and qPCR was used to detect the expression of drug targets in DFUs.ResultsFirstly, we used the Cell Maker database to obtain information about human cells and related gene markers, and manually reviewed a total of 45 kinds of cells and maker information that may appear in the DFUs microenvironment, which were divided into 17 cell clusters after annotation. Subsequently, we counted the proportions of DM and DFUs in different types of cells, and the results showed that the proportions of macrophages, white blood cells, and monocytes were higher in patients with DFUs, while the proportions of pluripotent stem cells and stromal cells were higher in patients with DM. The Pseudo-time series analysis of cells in DFUs showed that the differentiation pathways of immune cells, mesenchymal cells and stem cells were similar in the three states, while the other cells were distributed in different stages. At the level of a single cell, the scores of both multipotential stem cells and hematopoietic stem cells were significantly lower in DFU healing and non-healing than in DM. Additionally, the highly expressed genes in DFU were chosen as drug targets. We identified seven potential target genes and discovered twenty drugs with high significance. Finally, the colocalization relationship between CD19, ITGAM, and HLA-DR expression in monocytes and macrophages of DFU skin tissue and healthy subjects was analyzed by laser confocal microscopy with the immunofluorescence triple labeling method. The results showed that the expressions of CD19, ITGAM, and HLA-DR in the skin of DFUs were significantly higher than those in the skin of healthy subjects, and the co-localization relationship was significant in DFUs.ConclusionThis study can serve as a resource for the treatment of DFUs.