bluuebunny/crossref_metadata_2025_split huggingface dataset:
Unexplained Multi-Site Thrombosis: A Step-by-Step Strategy for Factor V Leiden Detection in a Hypercoagulable Patient
Authors: Raberi Venus Shahabi , Kahourian Ozra , Faramarzzadeh Reza , Parizad Razieh , McConnell Oliver , Rostamzadeh Alireza 
Published: January 2025

Thrombotic manifestations involve the development of blood clots within blood vessels. These conditions can occur unexpectedly in different areas and are often linked to life-threatening situations, presenting challenges for both diagnosis and treatment.
We present a case of multi-site thrombosis in a patient with a confirmed hypercoagulable state, resulting from a positive factor V Leiden mutation. The patient’s medical history included hypertension, chronic obstructive pulmonary disease, previous thrombotic events, and changes in anticoagulant therapy.
This case highlights the challenges associated with multisystem thrombosis and underscores the necessity of employing various diagnostic techniques, such as echocardiography, computed coronary angiography, and Doppler ultrasonography. In this instance, the patient presented with a history of unprovoked lower limb deep vein thrombosis and multiple arterial thromboses.
The patient’s treatment regimen comprised anticoagulants, antiplatelet drugs, and vasodilators. While a reduction in thrombus size was noted, complete revascularization could not be attained.
Effective diagnosis and treatment of venous and arterial thrombosis often require multimodal imaging. Selective blood test screening can be beneficial in diagnosing or detecting inherited or acquired abnormalities linked to thrombosis development.
Iliopsoas Injections
Authors: Katz Luca , Feinberg Griffin , Kent Victoria , Quinn Matthew , Milner John D. , Tabaddor Ramin 
Published: January 2025

Background:
Iliopsoas injuries are a common cause of anterior hip and groin pain and can be successfully managed with conservative treatment. Corticosteroid and local anesthetic injections can also be offered in conjunction with nonoperative management. Given the variability in reported injection guidelines, composition, and techniques, the purpose of this study was to systematically review the literature to assess progression to surgery and patient outcomes following iliopsoas injections.

Methods:
Four online databases (PubMed, Cochrane Library, MEDLINE, and Scopus) were searched for studies investigating the outcomes of iliopsoas injections from database inception until January 2024 in accordance with the Preferred Reporting Items for Systematic Meta-Analyses guidelines. Three reviewers screened titles, abstracts, and full-text articles independently and in duplicate. Recorded data included demographic data, patient-reported outcomes, complications, injection traits, and progression to surgery.

Results:
Six articles were included in the review (follow-up time = 28.6 months). These studies included patients with iliopsoas bursitis, tendinopathy, and snapping hip. Five studies used the iliopsoas bursa as the injection target. All studies used local anesthetics in their injection formulations, with 5 also adding a corticosteroid. In 3 studies, the Numeric Rating Scale improved from preinjection (mean = 7.33) to postinjection (mean = 2.47). Three studies demonstrated an improvement in Harris Hip Score from a mean of 58.49 preinjection to 89.91 postinjection. Following injections, 28.9% (68/235) of patients progressed to surgery, with psoas tenotomy (38.3%, 26/68) being the most common procedure. There were no complications reported in all of the included studies.

Conclusion:
This study demonstrates that iliopsoas injections are a clinically effective treatment of a variety of pathologies, including bursitis, tendinopathy, and snapping hip, and have a low rate of complications. Physicians should consider using iliopsoas injections in patients whose symptoms are refractory to conservative management, including physical therapy.

Level of Evidence:

Level III. See Instructions for Authors for a complete description of levels of evidence.
Porosity and mineralogy in the Lajas tight gas sandstone reservoir, Neuquén Basin, Argentina
Authors: Lavauzelle Nathan , Siitari-Kauppi Marja , Sardini Paul , Loggia Didier , Sammaljärvi Juuso , Beaufort Daniel , Rainoldi Ana Laura , Minisini Daniel 
Published: January 2025

ABSTRACT
In specific subsurface conditions, the sandstones of the Lajas Formation represent a tight gas reservoir in a deltaic mouth-bar system. Therefore, understanding the diagenetic processes that affected these sandstones is a first step towards reservoir quality prediction and production optimization.14C-PMMA autoradiography can be used to characterize the porosity distribution at centimeter-to-micrometer scales. The mineral phases can be associated with specific porosities using scanning electron microscopy (SEM) in the backscattered-electron imaging mode (BSEi) and can be combined with elemental mapping using energy-dispersive X-ray spectroscopic (EDS) analysis. For bulk analysis, porosity mapping is useful for characterizing the constrictivity and the tortuosity of the porous network created by the geological processes related to the basin’s evolution (e.g., compaction, dissolution, fracturing, cataclasis). 14C-PMMA autoradiography offers a reliable porosimetry method which is adaptable to most rocks. In our study, the superimposition of porosity maps and mineral maps revealed that: 1) secondary porosity, deriving from feldspar dissolution in medium- to coarse-grained sandstone, and the varying degree of dissolution are both influenced by grain size and degree of cementation, 2) mudstone rip-up clasts have homogeneous porosities (3–4%), 3) the host rock’s porosity varies widely (1–8%), 4) bioturbated siltstone and sandstone present higher porosities within the trace fossils, mainly associated with organo-pores (primary porosity in the organic matter), mineral formation (pyrite precipitation), and clay development (microporosity). Integrating these petrophysical and mineralogical observations with the interpretation of the sedimentary and structural processes that affected the lithologies analyzed allows the following generalizations: 1) the highest porosity and permeability occur in samples representing high-energy environments of deposition (deltaic channel infills and mouth bars), 2) deformation bands cannot be considered fairways for fluid migration, as they have a lower porosity than the sandstone matrix, mainly because dilational shear bands underwent cementation and compactional shear bands underwent cataclasis.
Identification of Reservoir Heterogeneities Using Tracer Breakthrough Profiles and Genetic Algorithms
Authors: Guerreiro J.N.C. , Barbosa H.J.C. , Garcia E.L.M. , Loula A.F.D. , Malta S.M.C. 
Published: January 2025

Abstract
In this paper we describe a methodology to identify reservoir properties using numerical techniques which combine finite element methods, a streamline approach and genetic algorithms. As a model problem we consider the system of equations governing incompressible miscible displacement in two-dimensions whose solution furnishes tracer breakthrough profiles which are parametrized as function of the properties to be determined. Given a "population" of initial parameters, after the solution of the model problem and the comparison of each profile with a target one, we "evolve" to a new "population" following the concepts of genetic algorithms and according to the fitness of each member of the previous "population".
Characterization of Corrosion Inhibiting Emulsions for Continuous Well Treatment
Authors: Civan Faruk , Martin Richard L. , Weers Jerry J. 
Published: January 2025

Abstract
Field experience has proven that water or oil-external emulsions containing properly formulated corrosion and scale inhibitors and cleaners, placed downhole in sufficient quantities, can provide more effective and long term well treatment than the frequently used oil-based products. The gradual break-up of emulsions due to the downhole shearing and heating conditions of wells yields a means of controlled release of the active ingredients present in these emulsions over a long period of time.
The typical properties of corrosion inhibiting emulsions affecting the downhole placement of emulsions are measured and correlated by relevant theories. The mechanisms of the emulsion break-up and inhibitor release by various effects including shearing and heating are determined, formulated, and verified by experimental data~ The viscosity, drying and stability processes, surface tension and repetitive film coating on tubing and casing walls, inhibitor resolution and return profiles of the typical water and oil-external emulsions used in industry are correlated. A transient-state model for emulsion break-up and consumption, temperature, and the remaining and delivered inhibitor concentrations of downhole emulsion reservoirs is developed and verified by experimental data. This model is used to simulate typical scenarios and determine the sensitivity of the various parameters involved in well treatment.
Prevalence and associated factors of Needle Stick and sharp Injuries Among health care workers ın a teiıtiary care hospıtal Mogadishu, Somalia
Authors: isse Suad abdikarim , DOĞAN Ahmet , Çelik Ali kutta , Ali Tigad Abdisad , Wehlie Jabir Abdullahi , Sheikh Sowda Abdikarim , Ali Ahmed Mohamed , Hussein Lıban Ade , Adam Fatima Nor , TÜRKAN Mucahit 
Published: January 2025

Abstract

Background
Needle sticking and sharps injuries (NSSIs) are significant occupational hazards for healthcare workers (HCWs), contributing to the transmission of blood-borne pathogens such as hepatitis B, hepatitis C, and HIV. However, the prevalence and associated risk factors for NSSIs, particularly in low-resource settings, remain underexplored.
Materials and Methods
A cross-sectional survey was conducted with 334 healthcare workers in a tertiary care hospital in Mogadishu, Somalia, in which questionnaires were used to assess injury occurrence, demographic data, and safety practices.
Results
The majority (72.4%) were aged between 26 and 40 years, with males accounting for 55.6% of the study population. A significant proportion (59.5%) reported being single. Nurses and midwives constituted the largest professional category, representing 33.6% of the participants. Notably, 72.7% of the respondents reported working night shifts. With respect to educational qualifications, 64.6% held a bachelor’s degree, and 50% had between 6 and 10 years of professional experience.
Conclusion
This study highlights the alarming prevalence of NSSIs among healthcare workers in Somalia, emphasizing the occupational risks faced by nurses, followed by doctors. These findings underscore the urgent need for enhanced safety protocols, training, and awareness campaigns to mitigate these injuries and protect healthcare workers from blood-borne infections.
High host specificity of alphacoronaviruses in Nearctic, insectivorous bats
Authors: Kotwa Jonathon , Bhuinya Arkadeb , Yim Winfield , Gallo Giulia , Singh Pahul , Liu Qiang , Kumar Anil , Chien Emily , Hou Aaron , Chee Hsien-Yao , Blais-Savoie Juliette , Yip Lily , Von Zuben Valerie , Wouters K. Aidan , Misra Vikram , Lung Oliver , Bailey Dalan , Maguire Finlay , Banerjee Arinjay , Mubareka Samira , Davy Christina 
Published: January 2025

Abstract

Bats are reservoir hosts for a number of coronaviruses, some of which may pose spillover risks for humans and other animals. Surveillance for bat coronaviruses in temperate regions remains limited and represents an important blind spot for emerging pathogen preparedness and bat conservation. We detected two alphacoronaviruses in big brown bats (Eptesicus fuscus) and little brown myotis (Myotis lucifugus) in the province of Ontario, Canada. These viruses are closely related to other coronaviruses circulating in bats in North America and Asia and also related to human and swine coronaviruses. We found unexpected diversity in the spike gene of these highly similar coronaviruses. High homology in the receptor-binding domain (RBD) was maintained in viruses derived from the same species of bat, but markedly lower in those derived from other species. RBD in silico structural analysis of closely related coronaviruses suggests that the viruses we detected are less likely to use bat APN (30 bat species) or ACE2 (20 bat species), or human DPP4 or TMPRSS2 as putative receptors or attachment factors. To gain early insights into interferon antagonism, we also functionally characterized the accessory protein ORF3 from both bat viruses and discovered that ORF3 inhibited both IFNβ production and signaling. Taken together, our study provides insights into coronavirus diversity in Nearctic, insectivorous bats in a previously under-sampled region. This work provides a baseline for more in-depth surveillance to better characterize the transmission dynamics of endemic coronaviruses in free-ranging wildlife, and for exploring the evolutionary relationships between coronaviruses and their hosts.
Assessment of multi-strain probiotic exhibiting in vitro cholesterol-lowering, antioxidative and lipolytic properties
Authors: Chauhan Mehul , Modasiya Ishita , Maniya Hina , Mori Priya , Kumar Vijay 
Published: January 2025

Abstract

Various studies have been conducted reporting the hypocholesterolemic effect of individual probiotic strains while only a few such reports are available on multi-strain probiotic formulation. In this study, 23 indigenous isolated probiotics were used to formulate multi-strain probiotic consortium after evaluating their co-culture potential. The study evaluated the antioxidant, lipolytic, hypocholesterolemic, and synergistic attributes of probiotic isolates for selection of the be compatible strains for effective consortium formulation. Limosilactobacillus fermentum BAB 7912 showed the highest antioxidant activity (30.22% DPPH radical reduction potential and 87.21% ABTS radical scavenging potential) among strains growing synergistically. The other strain of the final consortium Bacillus rugosus PIC5CR had the highest ferric reducing antioxidant potential (81.45%). Another probiotic isolate Bacillus rugosus PIB9CR was able to produce highest quantity of p-nitrophenol (272.61 ± 0.54 µM/15 min) as determined by p-nitrophenyl acetate method, indicating the higher lipolytic activity for both whole-cells and cell free supernatant (CFS). Hence, the multi-strain probiotic consortium consisting of these three strains was formulated that showed the significant cholesterol reductions potential (70.07% after 24 hours and 74.77% after 48 hours) which was higher than any of the single isolates used in this study. The strains and probiotic consortium demonstrated satisfactory cholesterol reduction and lipolytic activity, with isolates having higher co-culture attributes. Further investigation is needed to determine their viability as well as efficacy in an in-vivo model.
Metronidazole-induced neurotoxicity: is iron a contributing factor?
Authors: Bariweni Moses W , Patel Vinood B , Zariwala Gulrez M , Ozolua Raymond I 
Published: January 2025

Abstract

Background
Metronidazole-induced neurotoxicity is a rising challenge in the management of susceptible infections. The mechanisms involved in metronidazole-induced neurotoxicity are not fully unraveled. This study was aimed at determining the effect of metronidazole on iron homeostasis in SH-SY-5Y neuroblastoma cells.
Methods
Confluent SH-SY-5Y neuroblastoma cells were treated with 1, 10, 25, 50, 100, 250 µM concentrations of metronidazole only or in combination with 20 µM iron. DMSO or culture media was used as control. Viability and ferritin assay were conducted on the treated cells. The treatments were for 24 hr, 48 hr and 72 hr respectively.
Results
In the viability assay, doses of metronidazole reduced viability of SH-SY-5Y neuroblastoma cells in a time and concentration dependent manner. After 24 hr treatment, 250 µM metronidazole reduced (P < 0.001) cell viability while 50 µM, 100 µM and 250 µM metronidazole reduced (p < 0.01, p < 0.001) viability only after 48 and 72 hr compared with control. Doses of metronidazole 50 µM, 100 µM and 250 µM in 20 µM iron reduced viability in a time dependent manner in all the tests periods. Metronidazole also induced a time and concentration dependent increase (P < 0.05) in cellular iron uptake in the 48 and 72 hr treated cells in concentrations above 25 µM metronidazole.
Conclusion
It is concluded that metronidazole induces a time and concentration dependent iron overload and consequent cell death in SH-SY5Y neuroblastoma cells and this may contribute to the mechanism of metronidazole-induced neurotoxicity.
An in vitro one-pot synthetic biology approach to simulating diverging Golgi O-glycosylation of tumor-associated MUC1 from normal tissue MUC1
Authors: Naidoo Kevin , Nashed Abdullateef , Dilsook Kyllen , Senapathi Tharindu 
Published: January 2025

Abstract

Peptide O-glycosylation relies on the coordinated action of glycosyltransferases (GTs) within the endoplasmic reticulum (ER) and Golgi apparatus. An in vitro one-pot synthetic biology approach was developed to investigate the specificity and kinetics of GT O-GalNAc glycosylation that leads to tumor antigen glycoforms of mucin 1 (MUC1). The focus is to experimentally simulate the divergent glycosylation pathways that lead to the synthesis of cancer-associated antigens (Tn, T) and their sialylated derivatives. First, the biosynthetic details of the defining first step of GALNT relocation from the ER to the Golgi was modeled using the one-pot method. Our findings reveal that an ER enriched with GALNTs results in complete Galnac (Tn) MUC1 site occupancy. This comes about as a function of two processes that are i) extended GALNT reaction time and ii) prevention of inhibition by subsequent glycosylation enzymes such as C1GALT1. The modeling confirms that B3GNT6 has negligible specificity for MUC1 Tn, explaining the absence of core 3 and core 4 structures in MUC1 in both normal and cancerous breast cell lines. Moreover, ST6GALNAC1, and not ST6GALNAC2, is primarily responsible for α-2-6 sialylation of Tn and T antigens. Computer reaction dynamic simulations combined with kinetic experimental analysis show that ST6GALNAC1 prefers fully glycosylated MUC1 and more importantly that its preference is to sialyate the S9 and T13 sites in the SAPDTR motif. This is especially the case when the MUC1 concentration is high (i.e., high-level of expression), suggesting that sTn upregulation on MUC1 in cancer is linked to the occupancy status of S9 and T13 glycosylated sites, that were previously found to be cancer-associated. The results from the one-pot synthesis approach presented here demonstrate its ability to simulate cellular glycosylation within the Golgi-ER. This systems modelling unpacks the molecular details of enzyme localization and substrate glycan occupancy that is fundamental to the regulatory mechanisms that gives rise to tumor-associated MUC1 antigens.
Wood-water relationship and micro-chemical Properties of Huminated Archaeological European Elm (Ulmus laevis P.)
Authors: Ghavidel Amir , Wang Shennan , Bak Miklós , Rautkari Lauri , Hosseinpourpia Reza 
Published: January 2025

Abstract

Archaeological wood requires effective conservation to prevent further degradation, and traditional modifications such as polyethylene glycol (PEG) have limitations, including hygroscopicity and chemical degradation over time. To explore alternative modification, this study was conducted to investigate the suitability of humins, crosslinked with different concentrations of succinic acid (SA), to protect archaeological elm wood from the Agapia Monastery against water and to determine the modification mechanism. Key parameters such as dimensional stability, moisture sorption isotherms, and determination of accessible hydroxyl groups as a function of humination modification were analyzed using dynamic vapor sorption (DVS). The modification mechanism was studied by microstructural and chemical properties evaluation by Confocal-Raman spectroscopy and scanning electron microscopy (SEM). Results indicated that humins, particularly crosslinked with SA, significantly improved the water-related properties of wood and its dimensional stability. The humination also reduced the accessibility of the hydroxyl group, thus decreasing the equilibrium moisture content (EMC) of huminated elm at RHs ranging from 0 to 95%. While SEM images revealed structural changes in the modified wood. Confocal-Raman spectroscopy confirmed the successful allocation of humins into the cell walls. This study demonstrates that humins are promising materials for archaeological wood conservation, providing improvements in both chemical and physical properties.
Liver regeneration following partial hepatectomy in hepatocellular carcinoma: not only size matters
Authors: Long Haiyi , Wu Qingyu , Huang Jiayao , Zhong Xian , Xie Yuhua , Duan Yu , Xie Wenxuan , Chen Zebin , Li Dongming , Xie Xiaoyan , Su Liya , Lin Manxia 
Published: January 2025

Abstract

Background
To investigate factors associated with liver regeneration capacity in patients with hepatocellular carcinoma (HCC) after liver resection.
Methods
A total of 123 patients with HCC who underwent liver stiffness (LS) measurement by two-dimensional shear wave elastography (2D-SWE) before liver resection were retrospectively included. The future liver remnant (FLR) and postoperative liver volume (PLV) were measured. The standardized future liver remnant (sFLR), parenchymal hepatic resection rate (PHRR), and regeneration index (RI) were calculated. Patients were classified into two subgroups based on the extent of resection. Spearman correlation analysis was used to investigate factors associated with RI, and the receiver operating characteristic (ROC) curve was employed to evaluate the diagnostic performance of these relative factors to predict a significant liver regeneration (RI > 1).
Results
Strong correlation between RI and volume-related parameters, including FLR (r=-0.754, p < 0.001) and sFLR (r=-0.811, p < 0.001), were identified. Optimal cutoff values of 535ml for FLR and 0.452 for sFLR were used to predict a significant liver regeneration with an area under the curve (AUC) value of 0.967 and 0.980, respectively (all p < 0.001). In the high PHRR (> 50%) subgroup, LS was the only non-volume parameter correlating with RI (r=-0.526, p = 0.012). A cutoff value of 6.7kPa was used to predict a significant regeneration with an AUC value of 0.767 (p = 0.035).
Conclusions
The remnant volume was the key to liver regeneration after resection for HCC. LS may serve as a potential predictor for liver regeneration in those with extensive loss of parenchyma.