bluuebunny/crossref_metadata_2025_split huggingface dataset:
Standardization of grocott`s methenamine (hexamine) silver method for glycogen demonstration in liver tissue
Authors: Idris Atheelah Mohammed , Elgamri Hibatalla Elshazli , Batran Samah Abdelrahim , Zakout Yosef Mohamed-Azzam 
Published: March 2023

Abstract
Purpose
Demonstration of glycogen can be done in different lesions and is considered diagnostically significant, mainly in some tumors. Glycogen staining is affected by the type of fixative, the temperature of fixation, and the staining technique.
Methods
Grocott`s methenamine (Hexamine) silver technique quality was assessed after 4 different types of fixatives at 2 different temperatures (Bouin's solution, 10% neutral buffered formalin (NBF), 80% alcohol, and Rossman's solution at room temperature (RT) and 4°C; for 24 hours). These variables were studied to optimize this technique for glycogen demonstration. Archived paraffin blocks were used in this study. They were prepared from one rabbit`s liver and thirty-two paraffin sections were prepared and stained with Grocott`s methenamine (hexamine) silver technique.
Results
Eighty percent ethanol gave better results at both RT and 4°C in comparison to the other fixatives.
Conclusion
80% alcohol at 4°C seems to provide the best staining results for glycogen with the Grocott methenamine (hexamine) silver technique at the level of this study.
Metabolic delineation of IDH1 wild-type Glioblastoma surgical anatomy: how to plan the tumor extent of resection
Authors: Altieri Roberto , Certo Francesco , Pacella Daniela , Cammarata Giacomo , Garozzo Marco , Broggi Giuseppe , Caltabiano Rosario , Magro Gaetano , Russo Giorgio , Cosentino Iano , Ippolito Massimo , Barbagallo Giuseppe 
Published: March 2023

Abstract
Purpose: Magnetic resonance imaging (MRI) is the current standard for preoperative planning of glioblastoma (GBM) surgery. However, recent data on the use of 11C-methionine positron emission tomography (11[C]-MET PET) suggest its role in providing additional information beyond MRI. The purpose of this study is to establish if there is a correlation between anatomical and metabolic data.
Methods: We retrieved all GBM cases treated at our institution from 2014 to January 2021. Preoperative MRI (Enhancing Nodule -EN-, FLAIR and Total Tumor Volume -TTV-) and PET volumes were evaluated to analyze potential correlations between anatomical and metabolic data. These findings were then correlated with pathological results obtained from the different radiological tumor regions.
Results: 150 patients underwent surgery for GBM and 49 of these were also studied preoperatively with 11[C]-MET PET; PET volume was evaluated in 47 patients. In 33 patients (70.21%) preoperative 11[C]-MET PET volume > preoperative EN volume and in 11 (23.4%) preoperative 11[C]-MET PET volume > preoperative TTV. We found a significant correlation between preoperative TTVs and PET volumes (p = 0.016) as well as between preoperative EN volumes and PET volumes (p = <0.001). Histologically, samples from ENs exhibited the conventional GBM morphology while samples from the FLAIR regions showed white matter tissue, with focal to diffuse tumor cells infiltration and areas of reactive astrogliosis.
Conclusion: In our series PET volumes correlated with EN and TTV on preoperative MRI, indicating that PET should be considered in the presurgical planning to achieve a supratotal resection.
Two Approaches to Estimate the Shapley Value for Superadditive Partially Defined Games
Authors: Masuya Satoshi 
Published: March 2023

Abstract
By Willson [1993], partially defined cooperative games (PDGs in short) are first considered. A PDG is a cooperative game in which the worth of some coalitions is unknown. We investigated superadditive games and Shapley values on a class of PDGs in Masuya [2016]. We showed that the set of the superadditive full games that can be obtained from a given PDG is a polytope and showed all its extreme points (Theorem 2, Masuya [2016]). However, it is not correct despite being a very important theorem in the paper. However, in this paper, we consider alternative approaches to resolve this problem.
JEL Classification Code: C710
Voice Patterns for Classification and Regression of Atherosclerotic Coronary Disease
Authors: Patrício Mélissa , Ferreira Nuno Dias , Morais Gustavo , Coelho Luís 
Published: March 2023

Abstract
Background: Cardiovascular diseases are the main cause of mortality in both genders, being coronary artery disease the most prevalent type. Risk factors provide a limited help to estimate the presence of disease, acknowledging the need to investigate new techniques or biomarkers. In this study, it is our aim to evaluate the feasibility of using acoustic patterns of European Portuguese to infer about coronary disease;
Methods: By collecting audio signals from patients diagnosed with heart disease and healthy subjects, a new database was developed, consisting of audio recordings and clinical metadata from a total of 84 participants. Using a combination of acoustic features, risk factors and clinical information, with distinct machine learning models, we explored binary classification and regression;
Results: The Random Forests algorithm allowed to achieve a top accuracy of 88% for a binary classification (healthy vs disease) and ElasticNet allowed to achieve the minimum mean absolute error, 0.14, in a regression task. Fewer observations in higher CAD-RADS categories were limiting challenges;
Conclusions: A combination of speech biomarkers and medical information can be used for identifying patterns of atherosclerotic coronary disease.
Analysis of precipitation and drought trends by a modified Mann–Kendall method: a case study of Lorestan province, Iran
Authors: Lornezhad Ehsan , Ebrahimi Hossein , Rabieifar Hamid Reza 
Published: March 2023

Abstract
In this study, to evaluate the trend of precipitation change, the Mann–Kendall method has been used. The studied area is Lorestan province located in the western part of Iran. To achieve this goal, time series of annual and monthly rainfall data were collected for different statistical periods. Moreover, in order to analyze the drought, the standard precipitation index and the non-parametric Mann–Kendall test were used. To predict the meteorological drought in this province, the monthly time series of the precipitation parameter was incorporated. The results showed that most parts of Lorestan province are facing an extreme drought and such conditions will happen again in the future. Furthermore, the amount of precipitation was predicted until 2032, and the trend of predicted precipitation data in the entire Lorestan province showed that there is a significant trend in most months. The results of the research on an annual scale showed that all stations have a significant negative trend at the level of 5%, which indicates the existence of a negative trend, or in other words, a decrease in irrigation in the studied stations. Therefore, according to the obtained results, it is necessary to plan water consumption in Lorestan province toward sustainable management.
Circulating Tumor Cells and Cell-free Nucleic Acids as Biomarkers in Colorectal
Cancer
Authors: Pourali Ghazaleh , Khalili-Tanha Ghazaleh , Nazari Elham , Maftooh Mina , Nassiri Mohammadreza , Hassanian Seyed Mahdi , Mobarhan Majid Ghayour , Khazaei Majid , Ferns Gordon , Avan Amir 
Published: March 2023

Abstract:
Colorectal cancer (CRC) is currently the second most prevalent cancer diagnosed in women and the
third most common kind of cancer in men. Despite tremendous efforts and advancements in diagnostic approaches
and treatment options, the mortality rate of CRC accounts for around one million each year globally.
The five-year survival rate of CRC is reported to be approximately 14 percent for patients diagnosed at an advanced
stage. Due to its significant associated mortality and morbidity, diagnostic tools to identify the disease
at its early stages are urgently required. Early diagnosis may lead to better outcomes. The gold standard approach
for CRC diagnosis is colonoscopy with biopsy. However, it is an invasive process with a risk of complications
and discomfort for the patient. Moreover, it is usually performed in symptomatic or high-risk individuals
and therefore, asymptomatic patients might be missed. Thus, alternative non-invasive diagnostic techniques
are required to improve CRC outcomes. The new era of personalized medicine is identifying novel biomarkers
associated with overall survival and clinical outcomes. Recently, liquid biopsy, a minimally invasive analysis of
body fluid biomarkers, has gained attention for diagnosis, evaluation of prognosis, and follow-up of patients
with CRC. Several previous studies have demonstrated that this novel approach allows for better understanding
of CRC tumor biology and leads to an improvement in clinical outcomes. Here, we explain the enrichment
and detection methods of circulating biomarkers, including CTCs, ctDNA, miRNA, lncRNA, and circRNA.
Furthermore, we provide an overview on their clinical potential as diagnostic, prognostic, and predictive
biomarkers for CRC.
Unveiling Role of MicroRNAs in Metastasizing Triple Negative Breast
Cancer: From Therapeutics to Delivery
Authors: Arya Vedpriya , Balkrishna Acharya , Mittal Rashmi 
Published: March 2023

Abstract:
Triple negative breast cancers are malignant, heterogeneous tumors with high histological
grades, increased reoccurrence, and cancer-related death rates. TNBC metastasis to the brain,
lungs, liver, and lymph nodes is a complex process regulated by epithelial to mesenchymal transition,
intravasation, extravasation, stem cell niche, and migration. Aberrant expression of miRNAs,
also known as a transcriptional regulators of genes, may function as oncogenes or tumor suppressors.
In this review, we systematically elucidated the biogenesis and tumor suppressor role of miRNA
in targeting distant metastasis of TNBC cells and the above-mentioned underlying mechanisms
involved in complicating the disease. Apart from their therapeutic implications, the emerging roles
of miRNAs as prognostic markers have also been discussed. To overcome delivery bottlenecks,
RNA nanoparticles, nano-diamonds, exosomes, and mesoporous silica nanoparticle-mediated delivery
of miRNAs have been contemplated. Altogether, the present review article uncovers the potential
role of miRNA in antagonizing distant metastasis of TNBC cells, and highlights their clinical
significance as prognostic markers and possible drug delivery strategies to enhance the likely outcome
of miRNA-based therapy against the disease.
Point-of-Care HIV Test for a Promising Simple and Rapid Clinical HIV
Definite Diagnosis Process
Authors: Yao Chunyan , Xue Zhenrui , Song Min , Peng Ping 
Published: March 2023

Background:
This study compared and evaluated the performance of a commercially available HIV POC rapid test with assays commonly used in clinical laboratories, including enzyme-linked immunosorbent assay (ELISA), western blot (WB), and reverse transcription-polymerase chain reaction (RT-PCR).

Methods:
500 patients’ samples were detected by the POC rapid test and clinically common tests (WB, ELISA, and RT-PCR) to compare detection performance, test time, and test cost.

Results:
Taking the WB results as the gold standard, the results of RT-PCR were completely consistent with WB. The concordance of ELISA and POC with WB was 82.00% and 93.80%, respectively, with statistically significant differences (p<0.05).

Conclusion:
This study provides evidence that rapid HIV POC assays are superior to ELISA and that WB and RT-PCR have equal detection performance in detecting HIV. As a result, a rapid and cost-effective HIV definition process based on the POC assays can be proposed.
Application of Nanotechnology for Herbal Medicine Development: A Review
Authors: Anand Anubhav , Gautam Preety , Ojha Smriti 
Published: March 2023

Background:
Herbal medicines have been extensively used to treat diseases since the prehistoric
era, but written records date back more than 5,000 years only. All civilizations developed their
knowledge of herbal medicines in a well-ordered system, such as Ayurveda, Unani, Traditional Chinese
Medicine, etc. The interest in traditional medicine declined after the discovery of modern medicine. However,
in the 21st century, herbal medicines are staging a comeback as the dangers and limitations of modern
medicine have become more apparent and herbal medicines are viewed as a balanced and moderate
approach to healing.

Methods:
This review includes the nanoformulations of phytoconstituents and extract. Advancements in
analysis and clinical research prove the efficacy of nano-herbal medicines in preventing and treating diseases.
This review is mostly about how nanotechnology can be used to help herbal medicines work better.

Result:
The major problems with herbal medicines are their poor solubility and stability. New technological
advancements are capable of removing the problems associated with herbal medicine. Novel drug
delivery systems such as microemulsion, liposome, niosome, and nano-drug delivery systems are used to
enhance the safety and efficacy of herbal medicines.

Conclusion:
Nanotechnology has significant merit for herbal medicines, such as improving solubility,
bioavailability, pharmacological activity enhancement, and stability enhancement.
Trace Element Levels in Rice Varieties Consumed in Saudi Arabia
Authors: Ashraf Muhammad Waqar , Alanezi Faisal 
Published: March 2023

Background:
Rice is the most available and consumed food in the world, and is considered
one nutritious food. Due to the same reason, rice is the popular food in Saudi Arabia, being
consumed with vegetables and non-vegetable recipes.

Methods:
Taking the nutritive value of rice into consideration, the metal analysis was performed,
and both essential and heavy elements were detected in seven varieties of rice, viz, Paddy Rice,
Brown Rice, White Rice, Broken Rice, Parboiled Rice, Black Rice and Red Cargo Rice. Among
the heavy elements arsenic, cadmium, iron, lead and mercury were analysed and the concentration
of these elements was found within the value of tolerance.

Results:
The maximum concentration of all these elements was found below the limits set by
NSU and the FDA. The essential elements, which were analyzed in all seven varieties of rice, include
cobalt, magnesium, copper, selenium, manganese and zinc.

Conclusion:
All these elements were found in rice quantity, required for the normal metabolic
activities of the body. The varieties of rice analyzed have essential element concentrations in a
normal range.
Research Progress of Aging-related MicroRNAs
Authors: Chen Zhongyu , Li Chenxu , Huang Haitao , Shi Yi-Ling , Wang Xiaobo 
Published: March 2023

Abstract:
Senescence refers to the irreversible state in which cells enter cell cycle arrest due to internal or
external stimuli. The accumulation of senescent cells can lead to many age-related diseases, such as neurodegenerative
diseases, cardiovascular diseases, and cancers. MicroRNAs are short non-coding RNAs
that bind to target mRNA to regulate gene expression after transcription and play an important regulatory
role in the aging process. From nematodes to humans, a variety of miRNAs have been confirmed to alter
and affect the aging process. Studying the regulatory mechanisms of miRNAs in aging can further deepen
our understanding of cell and body aging and provide a new perspective for the diagnosis and treatment
of aging-related diseases. In this review, we illustrate the current research status of miRNAs in aging and
discuss the possible prospects for clinical applications of targeting miRNAs in senile diseases.
Choice and Timing of Antithrombotic after Ischemic Stroke,
Intracerebral Hemorrhage or Cerebral Venous Thrombosis
Authors: Ryan Dylan , Girotra Tarun , Feng Wuwei 
Published: March 2023

Abstract:
Stroke is a multifactorial vascular disease and remains a leading cause of disability in the
United States. Strokes can be ischemic or hemorrhagic in nature and secondary to arterial or venous
disease, making determining the etiology and secondary prevention strategy important for preservation
of the injured brain, prevention of recurrent strokes, and in the maintenance of good functional outcomes
for patients impacted by stroke. In this narrative review, we provide a synopsis of the available
medical evidence surround selection, timing, and choice of therapy, including utilization of left atrial
appendage closure, in patients with ischemic, hemorrhagic or venous stroke.
Metabolic Syndrome: The Constellation of Co-morbidities, A Global Threat
Authors: Madan Kirtika , Paliwal Sarvesh , Sharma Swapnil , Kesar Seema , Chauhan Neha , Madan Mansi 
Published: March 2023

Background:
Metabolic syndrome, also referred to as Syndrome X or obesity syndrome
is a cluster of diseases prevalent worldwide in both developed and developing countries. According
to WHO, it is referred to as a pathological condition wherein multiple disorders are manifested in the
same individual. These include hypertension, hyperglycemia, dyslipidemia and abdominal obesity.

Aims::
Metabolic syndrome is one of the most serious non-communicable health
hazards that have gained pivotal importance in the present scenario. The increasing prevalence affecting
around 25 % of the world populace, mainly attributes to the acceptance of western culture,
i.e. the intake of high-calorie food along with a substantial decrease in manual labor and adoption of
sedentary lifestyles. Therefore, its timely prevention and management are the dire need in the present
scenario.

Methods:
For successful accomplishment of the present review, an exhaustive analysis was performed
utilizing a pool of previous related literature. The terms used during the search included ‘metabolic
syndrome, prevalence, etiology, current pharmacotherapy for metabolic syndrome, etc. PUBMED,
Medline and SCOPUS were explored for the study of abstracts, research and review papers in
the quest for related data. The articles were downloaded and utilized for a meta-analysis study approach.

Conclusion:
In this review, an attempt was made to apprehend and summarize the epidemiology and
treatment strategies for metabolic syndrome with a better understanding of its pathogenesis. It was
postulated that an early diagnostic approach and subsequent line of treatment is required to prevent
the deterioration of an individual’s health and life.
A Health App for Evidence-Based Postpartum Information: Development and Validation Study
Authors: Silva de Medeiros Máyra Cármem , de Oliveira Maiara Costa , Gurgel Leonandro Valério Barbosa , Ribeiro Rodrigues Anna Giselle Câmara Dantas , Micussi Maria Thereza Albuquerque Barbosa Cabral , Magalhães Adriana Gomes 
Published: March 2023

Background
After childbirth, women undergo substantial physical and emotional changes. Therefore, it is important to provide them with information that helps them identify what is expected during this stage, as well as signs and symptoms that indicate complications after they have been discharged from the hospital.

Objective
This study aimed to develop a health app—Towards Motherhood—that provides evidence-based information about the postpartum period and evaluate the usability of the app with the target population.

Methods
This was a validation study involving 80 participants, including 24 professionals from the obstetric health field, 15 professionals from the technology field, and 41 postpartum women. The app was developed using React Native technology. Health professionals evaluated the app’s content using the Content Validity Index, technology professionals completed a validated evaluation to assess the appearance of the app, and postpartum women completed the System Usability Scale (SUS) to measure the usability of the app.

Results
The measurement of content validity using a Likert scale obtained an approval score of 99%. Regarding the app’s appearance, 92% of responses were positive, reflecting favorable approval. The SUS usability score was 86.2, which represents excellent acceptance.

Conclusions
The Towards Motherhood mobile app is a valid tool for promoting self-care during the postpartum period. The app’s evidence-based information, user-friendly design, and high usability make it an essential resource for women during this critical stage of their live.
Use of a Mobile Biofeedback App to Provide Health Coaching for Stress Self-management: Pilot Quasi-Experiment
Authors: Son Changwon , Hegde Sudeep , Markert Carl , Zahed Karim , Sasangohar Farzan 
Published: March 2023

Background
Mental health is an increasing concern among vulnerable populations, including college students and veterans.

Objective
The purpose of this study was to determine if mobile health technology combined with health coaching can better enable a user to self-manage their mental health.

Methods
This study evaluated the mobile app “Biofeedback” that provided health coaching on stress self-management for college student veterans’ mental health concerns. Twenty-four college student veterans were recruited from a large public university in Texas during the spring 2020 semester, impacted by COVID-19. Ten participants were assigned to the intervention group where they used the mobile Biofeedback app on their smartphones and smartwatches, and 14 were assigned to the control group without the app; assignment was based on mobile phone compatibility. Both groups participated in one initial lab session where they learned a deep-breathing exercise technique. The intervention group was then asked to use the mobile Biofeedback app during their daily lives and a smartwatch, and the control group was asked to perform the breathing exercises on their own. Both groups filled out Patient Health Questionnaire (PHQ-9) and Generalized Anxiety Disorder (GAD-7) self-assessments at 2-week intervals. At the end of the semester, both groups were given an exit interview to provide user experience and perceived benefits of health coaching via the mobile biofeedback app.

Results
The deep-breathing exercise in the initial lab session reduced stress in both groups. Over the course of the study, the app recorded 565 coached breathing exercises with a significant decrease (approximately 3 beats per minute) in participants’ heart rate during the 6-minute time period immediately after conducting the breathing exercises (Spearman rank correlation coefficient –0.61, P<.001; S=9,816,176). There was no significant difference between the two groups for PHQ-9 and GAD-7 scores over the course of the semester. Exit interview responses indicated that participants perceived that the mobile Biofeedback app improved their health and helped them address stress challenges. All participants reported that the intervention helped them manage their stress better and expressed that health coaching via a mobile device would improve their overall health.

Conclusions
Participants reported a positive perception of the app for their mental health self-management during a stressful semester. Future work should examine long-term effects of the app with a larger sample size balanced between male and female participants, randomized participant allocation, real-time detection of mental health symptoms, and additional features of the app.