bluuebunny/crossref_metadata_2025_split huggingface dataset:
Cultivation, cryopreservation and resuscitation of Theileria annulata transformed cells in serum-free media
Authors: Elati Khawla , Zweygarth Erich , Mhadhbi Moez , Darghouth Mohamed Aziz , Nijhof Ard M. 
Published: December 2022

IntroductionTropical theileriosis is a protozoan disease caused by Theileria annulata that affects cattle in Northern Africa, the Middle East and Asia where vector ticks of the genus Hyalomma occur. Various measures are applied to control the disease, including vaccination with attenuated T. annulata schizonts. Cultivation of T. annulata schizonts is mainly conducted in media containing Fetal Bovine Serum (FBS), which has some disadvantages such as costs, batch- to-batch variation and ethical concerns.MethodsIn this study, we conducted three experiments to evaluate the ability of (1) T. annulata strains grown in RPMI with 10% FBS (RPMI-FBS) to adapt and grow in serum-free media (i.e., HL-1, RPMI without FBS supplementation, ISF-1, and M199), (2) a T. annulata strain grown in ISF-1 and subsequently frozen in this medium to grow in ISF-1 again after long-term storage in liquid nitrogen, and (3) a T. annulata strain freshly isolated from infected bovine lymphocytes to growin ISF-1, also after cryopreservation. Cell numbers, schizont index, the viability and generation doubling time were calculated in all experiments.Results and discussionIn the first experiment, the Hessiene and Beja cell lines from Tunisia previously cultivated in RPMI-FBS and adapted to serum-free media continued to grow significantly better in RPMI-FBS compared to the serum-freemedia. In the second experiment, a Tunisian cell line (Hessiene) cryopreserved in ISF-1 with 5%[v/v] dimethylsulfoxide (DMSO) grewbetter after thawing in RPMI-FBS compared to ISF-1 with a highly significant difference in cell growth (p &lt; 0.001), whereas the third experiment showed that the Ankara cell line had similar growth characteristics in both RPMI-FBS and ISF-1 before and after thawing, with a shorter generation doubling time in ISF-1 than in RPMI-FBS (p = 0.23). Our findings suggest that freshly isolated cells can be propagated, frozen and thawed in serum-free media such as ISF-1, but once cells are adapted to cultivation in the presence of FBS or resuscitated from frozen storage, propagation in serum-free media may not perform as well as cultivation in RPMI-FBS.
Rapid Review of COVID-19 Vaccination Access and Acceptance for Global Refugee, Asylum Seeker and Undocumented Migrant Populations
Authors: Nichol Ariadne A. , Parcharidi Zoi , Al-Delaimy Wael K. , Kondilis Elias 
Published: December 2022

Objectives: Refugees, asylum seekers, and undocumented migrants globally have been disproportionally impacted by COVID-19. Vaccination has been a major tool to reduce disease impact, yet concerns exist regarding equitable allocation and uptake.Methods: A rapid literature review was conducted based on PRISMA guidelines to determine COVID-19 vaccination acceptance rates and level of access for these population groups globally.Results: Relatively high COVID-19 vaccine acceptance levels were commonly reported in these populations, although, trust in host governments was a frequently expressed concern, especially for undocumented migrants. Outreach efforts and access to comprehensive information from a trusted source and in appropriate language were found to be major determinants of COVID-19 vaccine acceptance. COVID-19 vaccination access and policies varied considerably across host countries despite urgings by international organizations to include migrants and refugees. While most governments endorsed inclusive policies, evidence of successful program implementation was frequently lacking, creating difficulty to better tailor and implement COVID-19 outreach programs.Conclusion: This review identifies impactful improvements to be implemented to ensure equitable COVID-19 vaccinations and to reduce disease burden on refugees, asylum seekers, and undocumented migrants.
A Mad7 System for Genetic Engineering of Filamentous Fungi
Authors: Vanegas Katherina Garcia , Rendsvig Jakob Kræmmer Haar , Jarczynska Zofia Dorota , Cortes Marcio Vinicius de Carvalho Barros , van Esch Abel Peter , Morera-Gómez Martí , Contesini Fabiano Jares , Mortensen Uffe Hasbro 
Published: December 2022

The introduction of CRISPR technologies has revolutionized strain engineering in filamentous fungi. However, its use in commercial applications has been hampered by concerns over intellectual property (IP) ownership, and there is a need for implementing Cas nucleases that are not limited by complex IP constraints. One promising candidate in this context is the Mad7 enzyme, and we here present a versatile Mad7-CRISPR vector-set that can be efficiently used for the genetic engineering of four different Aspergillus species: Aspergillus nidulans, A. niger, A. oryzae and A. campestris, the latter being a species that has never previously been genetically engineered. We successfully used Mad7 to introduce unspecific as well as specific template-directed mutations including gene disruptions, gene insertions and gene deletions. Moreover, we demonstrate that both single-stranded oligonucleotides and PCR fragments equipped with short and long targeting sequences can be used for efficient marker-free gene editing. Importantly, our CRISPR/Mad7 system was functional in both non-homologous end-joining (NHEJ) proficient and deficient strains. Therefore, the newly implemented CRISPR/Mad7 was efficient to promote gene deletions and integrations using different types of DNA repair in four different Aspergillus species, resulting in the expansion of CRISPR toolboxes in fungal cell factories.
Posicionamiento de empresa mercantil en Tabasco
Authors: Sanchez-Trinidad Rosa del Carmen , Sosa-Peña Rocio Guadalupe , Sánchez-Trinidad Adriana del Carmen 
Published: December 2022

Estrategias de marketing y publicidad para incrementar el posicionamiento de la empresa mercantil y aumentar la visibilidad en el mercado para así llegar al usuario potencial.
La principal problemática de este giro de empresa es que no cuenta con ninguna estrategia de publicidad y mercadotecnia. Toda empresa tiene su propio plan estratégico para el logro de sus objetivos y metas para alcanzar, estos pueden ser a diferentes plazos dependiendo la amplitud y magnitud de cada organización.
Observando la gran oportunidad de los sectores que tiene para ofrecer, en diversas ciudades del país, se diseñará una propuesta de un plan estratégico de marketing, enfocado en publicidad para aumentar la presencia en el mercado y de esta manera incrementar las ventas para acrecentar los ingresos.
Pensamento computacional na educação básica e o uso do software Scratch
Authors: Lauro Brugger Rafael , Scortegagna Liamara 
Published: December 2022

Este artigo faz um recorte do Trabalho de Conclusão de Curso (TCC) intitulado Pensamento computacional na educação básica e o uso do software Scratch apresentado no Curso de Licenciatura em Computação da Universidade Federal de Juiz de Fora no ano de 2022. O objetivo deste artigo é analisar como a utilização do software Scratch pode auxiliar no desenvolvimento do ensino do Pensamento Computacional na disciplina de Matemática. A pesquisa é de abordagem qualitativa, e utilizou-se de uma Revisão Sistemática de Literatura (RSL) entre os anos de 2012 à 2022 e a aplicação de um questionário para professores do Departamento de Matemática de uma escola pública do Estado de Minas Gerais (MG). As respostas ao questionário e os textos encontrados na RSL mostraram que os professores reconhecem a necessidade de se trabalhar com os estudantes os conceitos do Pensamento Computacional, assim como apresentaram o software Scratch como uma das principais ferramentas para trabalhar estes conceitos.
Cortar por lo más fino. La precariedad del trabajo de las enfermeras, exposición a las violencias, conflictos y estrategias de cuidado
Authors: Pasero Victoria , Carabaca Carla 
Published: December 2022

En este artículo abordaremos las condiciones de trabajo de cuidados de enfermeras, las violencias que experimentan en tanto trabajadoras, y las estrategias de cuidados y resistencias que tejen entre ellas; en el marco de relaciones de trabajo asalariado del sistema sanitario público y privado de la Región de Cuyo, Argentina.
Partimos desde un punto de vista feminista crítico y de los aportes provenientes de la confluencia disciplinar entre la sociología de las relaciones sociales de sexo, la psicodinámica del trabajo, el feminismo materialista y las teorías del cuidado. El análisis se desarrolla bajo una metodología cualitativa, y se utiliza la entrevista en profundidad para rastrear en los relatos las condiciones laborales y las violencias que padecen en tanto mujeres en la división sexual del trabajo.
En el contexto atravesado por la pandemia del COVID-19, se expuso con más fuerza la precariedad y vulnerabilidad de nuestras vidas, a la vez que la centralidad que tienen los trabajos de cuidados. Frente al fortalecimiento de un sentido policíaco y de control del cuidado, procuramos aportar herramientas que complejicen la comprensión de la naturaleza de los cuidados, en una mirada que propone integrar discursos, subjetividades, emociones, relaciones de poder y significaciones.
ADHERENCE OF COVID-19 PATIENT ACTIVITY DURING SELF-ISOLATION/QUARANTINE
Authors: Sulistiawati Sulistiawati 
Published: December 2022

Penyakit virus corona telah menyebar dengan cepat ke seluruh Indonesia. Kebijakan isolasi telah diberlakukan untuk menekan peningkatan jumlah kasus. Namun, tidak semua bisa mematuhinya kata demi kata. Kebaruan penelitian ini adalah meneliti tentang kepatuhan pasien covid-19 menjalani aktivitas selama isolasi mandiri/karantina. Penelitian ini dilakukan berdasarkan sejauh mana pemahaman tentang isolasi mandiri pada pasien COVID-19 dan mengevaluasi kekurangan selama isolasi mandiri. Penelitian ini merupakan penelitian potong lintang dengan desain cross-sectional melalui penyebaran kuesioner dan analisis dilakukan secara deskriptif. Jumlah sampel penelitian ini adalah 165 responden yang merupakan penderita atau pernah menderita COVID-19 di Indonesia. Yang dinilai dari responden adalah protokol kesehatan dasar COVID-19, protokol dasar isolasi mandiri, aktivitas sehari-hari selama isolasi mandiri, persiapan pengobatan. Sebagian besar responden sudah mematuhi protokol. Namun, perlu dicatat bahwa kegiatan dan protokol isolasi diri wajib tidak dipatuhi sepenuhnya di seluruh responden. Kesimpulannya, 14 responden (8.48%) anggota masyarakat tidak dapat melakukan isolasi mandiri dengan benar karena sebagian dari mereka masih belum memenuhi persyaratan isolasi diri yang benar.Kata kunci : COVID-19; Isolasi Mandiri; Karantina; Kepatuhan Abstract Coronavirus disease has spread rapidly throughout Indonesia. Policy for isolation has been enacted for repressing the number increases of cases. However, not all could comply with them words-by-words. The novelty of this study is to examine the compliance of Covid-19 patients undergoing activities during self-isolation/quarantine. This study is done based on how far is the understanding of self-isolation in COVID-19 patients and evaluate the flaw during the self-isolation. This research is a cross-sectional study design through the distribution of questionnaires and the analysis will be carried out descriptively. The samples were 165 respondents that has or had contacted COVID-19. The subjects that was rated are basic COVID-19 health protocol, basic protocol of self-isolating, daily activities during self-isolating, preparation for self-isolating treatment. Most of the respondent has mostly already obeyed. However, it should be noted that compulsory self-isolation activities and protocol are not obeyed fully across the respondents. In conclusion, about 14 respondents (8.48%) community could not carry out the proper self-isolation since some of them still did not fulfill the requirements of proper self-isolation.
THE RELATIONSHIP OF PHYSICAL ACTIVITY WITH THE QUALITY OF HEALTH OF ADOLESCENTS IN SELATAN MINAHASA DISTRICT
Authors: Kumayas Mira , Manampiring Aaltje Ellen , Mantjoro Eva Mariane , Posangi Jimmy , Wariki Windy 
Published: December 2022

AbstrakSaat ini kualitas hidup menjadi salah satu kriteria yang digunakan untuk mengevaluasi intervensi pelayanan kesehatan. Remaja menjadi salah satu penentu dalam meningkatkan kualitas hidup masyarakat. Kebaruan dalam penelitian ini karena meneliti tentang hubungan aktifitas fisik dengan kualitas hidup kesehatan remaja di Kabupaten Minahasa Selatan. Tujuan penelitian ini ialah untuk mengalisa hubungan aktifitas fisik dengan kualitas hidup kesehatan remaja mulai dari kesehatan fisik, psikologis, sosial dan kesehata lingkungan di Kabupaten Minahasa Selatan. Penelitian ini menggunakan metode penelitian kuantitatif dengan pendekatan cross-sectional yang dilakukan pada bulan September sampai Desember 2021 di SMP dan SMA dengan jumlah sampel 431 responden menggunaka uji Kruskall Wallis. Hasil penelitian menunjukan bahwa untuk domain fisik p=0,863; domain psikologis p=0,967; domain sosial p=0,638; dan domain lingkungan p=0,725. Kesimpulan penelitian ini yaitu aktifitas fisik tidak berhubungan dengan kualitas hidup kesehatan remaja di Kabupaten Minahasa SelatanKata Kunci : Aktifitas fisik; Kualitas Hidup, Remaja AbstractCurrently, quality of life is one of the criteria used to evaluate health care interventions. Adolescents are one of the determinants in improving the quality of life of the community. The novelty of this study is that it examines the relationship between physical activity and the quality of life of adolescent health in the South Minahasa Regency. The purpose of this study is to analyze the relationship between physical activity and the quality of life of adolescent health starting from physical, psychological, social, and environmental health in the South Minahasa Regency. This research uses quantitative research methods with a cross-sectional approach conducted from September to December 2021 in junior and senior high schools with a total sample of 431 respondents using the Kruskal Wallis test. The results showed that for the physical domain p = 0.863; psychological domain p=0.967; social domain p=0.638; and the environment domain p=0.725. The conclusion of this study is that physical activity is not related to the quality of life of adolescent health in South Minahasa RegencyKeywords: Physical activity; Quality of Life, Teens
STIGMA AGAINST PLWHA IN WEST SUMATRA
Authors: Tristanto Aris , Afrizal Afrizal , Setiawati Sri , Ramadani Mery 
Published: December 2022

AbstrakSumatera Barat merupakan provinsi ketiga dengan kasus stigma tertinggi terhadap ODHA pada tahun 2019. Kebaruan dalam penelitian ini adalah Stigma Terhadap ODHA Di Sumatera Barat. Menindaklanjuti data tersebut, maka penelitian ini bertujuan untuk mengetahui bagaimana gambaran stigma terhadap ODHA di Sumatera Barat pada tahun 2020 hingga 2021. Peneliti menggunakan metode kualitatif dengan pendekatan deskriptif. Pengumpulan data pada penelitian ini dilakukan melalui teknik wawancara dalam jaringan. Cara menentukan informan dalam penelitian ini adalah dengan menggunakan teknik pengambilan dengan cara snowball sampling. Penelitian ini menggunakan tiga pemeriksaan keabsahan data yaitu kredibilitas, ketergantungan,, dan kepastian. Hasil penelitian diketahui bahwa tahun 2020 ada satu penyebaran informasi status HIV salah seorang ODHA dari perawat kepada salah satu teman ODHA. Meskipun hanya satu kasus secara umum menurut LSM yang mendampingi ODHA, stigma kepada ODHA di tahun 2020 berkutat pada stigma dari masyarakat bahwa Coronavirus disease 2019 (COVID-19) sama dengan HIV. Berdasarkan catatan kasus yang dimiliki oleh LSM yang mendampingi ODHA, diketahui bahwa pada tahun 2021 terdapat empat kasus yang tergolong kepada stigma terhadap ODHA di Sumatera Barat. Terkait dengan hasil temuan kasus selama tahun 2020 sampai 2021 maka kelima kasus stigma tersebut mengarah pada stigma instrumental AIDS, yaitu refleksi ketakutan dan keprihatinan atas hal-hal yang berhubungan dengan penyakit mematikan dan menular.Kata kunci: Stigma; Masyarakat; ODHA. AbstractWest Sumatra is the third province with the highest cases of stigma against PLWHA in 2019. The novelty in this study is the Stigma Against PLHIV in West Sumatra. Following up on these data, this aims to find out how the description of research on PLWHA in West Sumatra from 2020 to 2021. Researchers used a descriptive approach. Data collection in this study was carried out through online interview techniques. The way to determine informants in this research is to use a sampling technique by means of snowball sampling. This study uses three checks of the validity of the data, namely dependability, dependence, and certainty. Based on the research, it is known that in 2020 there was a dissemination of information on the HIV status of an PLWHA from a nurse to a friend of PLWHA. Although there is only one case in general, according to the NGO that accompanies PLWHA, the stigma against PLWHA in 2020 revolves around the stigma from the community that Coronavirus disease 2019 (COVID-19) is the same as HIV. Based on case records owned by NGOs that accompany PLWHA, it is known that in 2021 there will be four cases classified as stigmatized against PLWHA in West Sumatra. Related to the findings of cases during 2020 to 2021, the five stigmatized cases lead to the instrumental stigma of AIDS, which is a reflection of fear over things related to deadly and infectious diseases.Keywords: Stigma; Public; PLWHA
EFFECT OF THE APPLICATION OF DEEP BREATH RELAXATION TECHNIQUES ON THE REDUCTION OF HYPERTENSION OF THE ELDERLY IN CILEUNGSI
Authors: Nasution Revie Fitria 
Published: December 2022

Hipertensi merupakan salah satu penyakit tidak menular yang dapat menyebabkan kematian. Mengategorikan penyakit ini sebagai the silent disease karena penderita tidak mengetahui dirinya mengidap hipertensi sebelum memeriksa tekanan darahnya. Kebaruan penelitian ini karena meneliti tentang pengaruh penerapan teknik relaksasi nafas dalam terhadap penurunan hipertensi lansia di Cileungsi. Penelitian yang dilakukan oleh penulis bertujuan untuk memperoleh hasil penelitian pada lansia rentang usia 60-65 tahun, memiliki masalah keperawatan hipertensi. Penelitian ini menggunakan desain kualitatif deskriptif dengan pendekatan studi kasus dan intervensi. Penelitian ini bertujuan untuk mengetahui pengaruh penerapan teknik relaksasi nafas dalam terhadap penurunan hipertensi lansia di Cileungsi desain penelitian cross sectional. Hasil penelitian ini mengungkapkan bahwa terdapat pengaruh yang signifikan antara teknik relaksasi nafas dalam terhadap hipertensi yang diukur dengan tensi darah berdasarkan hasil analisa Chi-Square dengan nilai significance person sebesar 0,00 atau lebih kecil dari 0,05. Kesimpulan penelitian ini yang menyatakan ada pengaruh antara pengaruh penerapan teknik relaksasi nafas dalam terhadap hipertensi di Cileungsi dapat diterima.Kata Kunci: Teknik Relaksasi Nafas Dalam; Hipertensi; Lansia Abstract Hypertension is a non-communicable disease that can cause death. Categorize this disease as the silent disease because the patient does not know he has hypertension before checking his blood pressure. The novelty of this study is because it examines the effect of the application of deep breath relaxation techniques on the reduction of hypertension of the elderly in Cileungsi. The research conducted by the author aims to obtain research results in the elderly aged 60-65 years, who have hypertension nursing problems. This study uses a descriptive qualitative design with a case study and intervention approach. This study aims to determine the effect of applying deep breathing relaxation techniques on reducing hypertension in Cileungsi with a cross sectional study design. The results of this study revealed that there was a significant effect between deep breathing relaxation techniques on hypertension, based on the results of Chi-Square analysis with a person significance value of 0.00 or less than 0.05. Thus, the hypothesis of this study which states that there is an influence between the application of deep breathing relaxation techniques on hypertension in Cileungsi can be accepted.Keywords: Deep Breathing Relaxation Techniqu; Hypertension; Elderly
THE INFLUENCE OF GIVING LAVENDER AROMA THERAPY AGAINST THE LEVEL OF INSOMNIAON ELDERLY AT BONGOPINI VILLAGE TILONGKABILA DISTRICT BONEBOLANGO REGENCY
Authors: Ilham Rosmin , Lasanuddin Hamna Vony 
Published: December 2022

Masalah insomnia pada lansia menyebabkan gangguan kemampuan intelektual, motivasi yang rendah dan ketidakstabilan emosional.Terapi lavender dianggap bermanfaat dan di kenal membantu meringankan nyeri, sakit kepala, insomnia, melawan kelelahan, stress dan meningkatkan daya tahan tubuh. Kebarauan penelitian ini karena melihat pengaruh pemberian aromatherapy lavender terhadap tingkat insomnia pada lansia. Tujuan dari penelitian ini untuk mengetahui pengaruh pemberian aroma therapy lavender terhadap tingkat insomnia pada lansia. Metode penelitian menggunakan desain quasi eksperiment dengan two group pre dan post test with control design. Jumlah populasi sebanyak 130 orang dengan teknik sampling menggunakan purposive sampling dengan jumlah sampel 26 respoden. Hasil penelitian menunjukan bahwa pada kelompok perlakuan di peroleh yang mengalami insomnia berat dengan keluhan sulit tidur secara terus menerus sebanyak 9 responden (69,2%), pada kelompok kontrol yang mengalami insomnia berat sebanyak 6 responden (42,6%). Setelah diberikan perlakuan yang tidak mengalami insomnia sebanyak 10 responden (76,9%) dan pada kelompok kontrol yang mengalami insomnia berat sebanyak 7 responden (53,8%), hasil analisis data menunjukan nilai pValue=0,0350,05. Kesimpulan pada penelitian ini terdapat pengaruh pemberian aroma therapy lavender terhadap tingkat insomnia pada lansia.Kata Kunci : Lansia; Insomnia dan Aroma Therapy Lavender. AbstractThe problem of insomnia in the elderly causes impaired intellectual ability, low motivation, and emotional instability. Lavender therapy is considered beneficial and is known to help relieve pain, headaches, insomnia, fight fatigue and stress and increase endurance. The novelty of this research is because it looks at the effect of giving lavender aromatherapy on the level of insomnia in the elderly. The purpose of this study was to determine the effect of giving lavender aroma therapy on the level of insomnia in the elderly. The research method used a quasi-experimental design with two groups of pre and post-test with a control design. The total population is 130 people with a sampling technique using purposive sampling with a total sample of 26 respondents. The results showed that in the treatment group, 9 respondents (69.2%) experienced severe insomnia with complaints of difficulty sleeping. In the control group, 6 respondents (42.6%). After being given treatment, there were 10 respondents (76.9%) who did not experience insomnia and 7 respondents (53.8%). This study concludes that there is an effect of giving lavender aroma therapy on the level of insomnia in the elderly.