bluuebunny/crossref_metadata_2025_split huggingface dataset:
Visual Color Change During Spin‐coating Guiding the Quality of Cu2ZnSn(S,Se)4 Films
Authors: Wang Zuoyun , Meng Rutao , Guo Hongling , Sun Yali , Xu Xuejun , Xu Han , Li Jianpeng , Zhang Yi 
Published: September 2023

AbstractSolution method provides a low‐cost and environmentally friendly route for the fabrication of Cu2ZnSn(S,Se)4 (CZTSSe) thin‐film solar cells. However, uncontrollable quality of the CZTSSe absorber layer will severely limit the device's performance. In this study, it is find that the thickness and the quality of the formed precursor is not stable because of the variation of the viscosity of the precursor solution. Combined by different characterization methods, the results disclose that such change is strongly related to the reflected color of the first coating layer during precursor growth. Further studies disclose that only by maintaining the appropriate reflected color can a well‐crystallized CZTSSe film be prepared, thereby obtaining good solar cell efficiency. This semi‐empirical pattern is confirmed by thin‐film interference theory. Under the guidance of this method, CZTSSe absorbers with high quality are obtained easily, and the highly efficient CZTSSe solar cell can be fabricated easily. This study provides a feasible and effective strategy to obtain the optimal structure and composition of CZTSSe film toward the production of highly efficient kesterite solar cells, which can also be widely applied to the preparation of other films by solution‐based method.
A new approach for the strength interaction diagram of FRP strengthened square steel reinforced high strength concrete columns
Authors: Mai Anh Duc , Sheikh M. Neaz , Nguyen Quang Trung , Pham Anh Duc , Hadi Muhammad N. S. 
Published: September 2023

AbstractThis paper proposed a new approach for the strength interaction diagram (N‐M diagram) of FRP strengthened square steel reinforced high strength concrete (SSRHSC) column, considering the influence of the applied load eccentricity on the ultimate conditions of FRP strengthened high‐strength concrete (HSC). The analytical model for the strength interaction diagram of FRP strengthened SSRHSC (FRPS‐SSRHSC) columns, which incorporated the stress–strain models of constitutive materials into the rectangular stress block (RSB) method, was developed and verified against test data. The strength interaction diagrams of FRPS‐SSRHSC columns developed based on the proposed analytical model matched well with the test results, while the strength interaction diagrams developed using available design codes overestimated the experimental strength interaction diagrams. The effect of confinement ratio and longitudinal FRP strengthening on the strength interaction diagrams was examined via parametric analyses. It was found that longitudinal strengthening had a negligible influence on the axial load and bending moment when the axial load was applied at a small eccentricity but had a substantial influence when the axial load was applied at a large eccentricity.
The role of LOXL2 induced by glucose metabolism-activated NF-κB in maintaining drug resistance through EMT and cancer stemness in gemcitabine-resistant PDAC
Authors: Lee Yun Sun , Kim Hyung Sun , Kim Hyo Jung , Kang Hyeon Woong , Lee Da Eun , Kim Myeong Jin , Hong Woosol Chris , Kim Ju Hyun , Kim Minsoo , Cheong Jae-Ho , Park Joon Seong 
Published: September 2023

Abstract
Gemcitabine is considered a standard treatment for pancreatic cancer, but developing drug resistance greatly limits the effectiveness of chemotherapy and increases the rate of recurrence. Lysyl oxide-like 2 (LOXL2) is highly expressed in pancreatic cancer and is involved in carcinogenesis and EMT regulation. However, studies on the role of LOXL2 in drug resistance are limited. Here, we investigated the mechanism of LOXL2 induction and the effect of LOXL2 on EMT and CSC in gemcitabine-resistant pancreatic cancer. Glucose metabolism was activated in gemcitabine-resistant pancreatic cancer cells, and NF-κB signaling was regulated accordingly. Activated NF-κB directly induces transcription by binding to the promoters of LOXL2 and ZEB1. The EMT process was significantly inhibited by the coregulation of ZEB1 and LOXL2. In addition, LOXL2 inhibition reduced the expression of cancer stemness markers and stemness by regulating MAPK signaling activity. LOXL2 inhibits tumor growth of gemcitabine-resistant pancreatic cancer cells and increases the sensitivity to gemcitabine in mouse models.

Key messages

We identified a specific mechanism for inducing LOXL2 overexpression in gemcitabine-resistant pancreatic cancer. Taken together, our results suggest LOXL2 has an important regulatory role in maintaining gemcitabine resistance and may be an effective therapeutic target to treat pancreatic cancer.
Voluntary wheel running during adolescence prevents the increase in ethanol intake induced by social defeat in male mice
Authors: Reguilón Marina D. , Ferrer-Pérez Carmen , Manzanedo Carmen , Miñarro José , Rodríguez-Arias Marta 
Published: September 2023

Abstract
Rationale
Exposure to social defeat (SD) induces a depressive phenotype, increased ethanol seeking and consumption, accompanied by activation of the neuroinflammatory response. However, a resilient response can be potentiated through physical exercise in the form of voluntary wheel running (VWR) during or after exposure to social stress. Therefore, the aim of this study was to test whether physical exercise during adolescence prior to being exposed to SD can enhance resilience to the increase in ethanol intake.

Methods
Male mice had access to VWR during adolescence and the effects of social defeat (4 sessions every 72 h) on oral ethanol self-administration (SA) was evaluated. Based on the social interaction test, mice were classified as resilient or susceptible to depressive-like behavior. Two weeks after the last encounter, mice were subjected to the drinking in the dark and oral ethanol SA paradigms. Mice were then sacrificed to measure brain-derived neurotrophic factor (BDNF) levels in the striatum and hippocampus.

Results
As expected, susceptible mice increased ethanol intake in the oral SA protocol. However, susceptible mice in the exercise condition did not increase ethanol intake, showing similar consumption and motivation for ethanol than the control and resilient groups. On the other hand, decreased BDNF levels were observed in susceptible mice in both experimental conditions compared to the control groups after ethanol SA.

Conclusions
The pre-exposure of VWR prevented the increase in consumption and motivation for ethanol induced by SD in susceptible mice. On the other hand, it appears that VWR did not exhibit any significant long-term effects on BDNF signaling, which is mainly affected in susceptible mice after ethanol intake.
Putrescine, in Combination with Gibberellic Acid and Salicylic Acid, Improves Date Palm Fruit Quality via Triggering Protein and Carbohydrate Accumulation and Enhancing Mineral, Amino Acid, Sugar, and Phytohormone Acquisition
Authors: Talaat Neveen B. , Nesiem Mohamed R. A. , Gadalla Ezz G. , Ali Shaimaa F. 
Published: September 2023

AbstractPutrescine (Put), gibberellic acid (GA3), and salicylic acid (SA) are involved in improving fruit growth and development. This is the first study investigating the effect of 1 mM Put, 100 mg L-1 GA3, and 100 mg L-1 SA on Phoenix dactylifera, cv. Zaghloul fruits. The experiment was conducted in a completely randomized block design during two successive seasons and five treatments [control (distilled water spray), Put, Put + GA3, Put + SA, and Put + GA3 + SA] were sprayed at the Hababouk (cell division) stage and Kimri [unripe green (cell elongation)] stage. Our results showed that all treatments significantly improved the yield and quality of ‘Zaghloul’ fruit by increasing the dry matter, crude fiber, ash, total soluble solids, (reducing, non-reducing, and total soluble) sugars, carbohydrate, protein, nitrogen, phosphorus, potassium, calcium, magnesium, sodium, zinc, iron, and manganese content as well as the peroxidase and catalase activity. Compared with the control treatment, exogenous applications also enhanced the amino acid (glutamic acid, aspartic acid, proline, glycine, alanine, arginine, cysteine, histidine, isoleucine, leucine, lysine, methionine, phenylalanine, serine, threonine, tyrosine, valine) acquisition and phytohormone (indole-3-acetic acid, cytokinins, GA3, SA) content, while decreasing the percentage of moisture, total acidity, total phenols, and tannins. The novel evidence indicates that among all treatments, application of diamine (Put), in combination with phytohormones (GA3 and SA), has the greatest effect on improving ‘Zaghloul’ fruit yield by up-regulating the nutrient acquisition, sugar accumulation, amino acid profile, antioxidant response, and phytohormone performance. These findings support the use of Put in conjunction with GA3 and SA to improve fruit yield and quality.
Circadian entrainment to red-light Zeitgebers and action spectrum for entrainment in the jewel wasp Nasonia vitripennis
Authors: Wang Yifan , Jin Lijing , Belušič Gregor , Beukeboom Leo W. , Wertheim Bregje , Hut Roelof A. 
Published: September 2023

AbstractLight is the most important environmental cue for the circadian system of most organisms to stay synchronized to daily environmental changes. Like many other insects, the wasp Nasonia vitripennis has trichromatic compound eye-based colour vision and is sensitive to the light spectrum ranging from UV to green. We recently described a red-sensitive, ocelli-based photoreceptor, but its contribution to circadian entrainment remains unclear. In this study, we investigated the possibility of Nasonia circadian light entrainment under long-wavelength red LED light–dark cycles and characterized the strength of red light as a potential Zeitgeber. Additionally, we measured the possibility of entrainment under various light intensities (from 5·1012 to 4·1015 photons·cm−2·s−1) and a broader range of wavelengths (455—656 nm) to construct corresponding action spectra for characterizing all circadian photoreceptors involved in photic entrainment. We also conducted electroretinogram (ERG) recordings for each wavelength in the compound eyes. Our findings demonstrate that Nasonia can entrain under red light dark cycles, and the sensory pathway underlying the red-light Zeitgeber response may reside in the ocelli. Combined with findings from previous research, we pose that blue- and green-sensitive rhodopsin photoreceptor cells function as the major circadian photoreceptors in both circadian entrainment by light–dark cycles and circadian phase shifts by light pulses, whereas the red-sensitive photoreceptor cell requires higher light intensity for its role in circadian entrainment by light–dark cycles.
Flow cytometric characterization of cecal appendix lymphocyte subpopulations in children: a pilot study
Authors: Arredondo Montero Javier , Torres López Andrea , Hurtado Ilzarbe Guillermina , Antona Giuseppa , Ros Briones Raquel , López-Andrés Natalia , Martín-Calvo Nerea 
Published: September 2023

Abstract
Introduction
Scientific literature regarding the characterization of lymphocyte subpopulations of the cecal appendix is sparse, with few precedents limited to immunohistochemical techniques.

Methods
We conducted a prospective pilot study to characterize lymphocyte subpopulations of the cecal appendix in children. Participants were divided into three groups: (1) patients without histological acute appendiceal inflammation, (2) patients with histological uncomplicated acute appendicitis, and (3) patients with histological complicated acute appendicitis (gangrenous, perforated). A fresh sample of the base of the appendix was taken from all patients and a flow cytometric study was performed. Quantitative variables were compared using Kruskal–Wallis test and Mann–Whitney U test.

Results
This study included 57 patients divided into Group 1 (n = 5), Group 2 (n = 37), and Group 3 (n = 15). Median values (IQR) of the percentage of B-lymphocytes were 67.8 [66.8–68.1] in group 1, 61.15 [53.74–66.4] in group 2, and 52.1 [33–62.02] in group 3 (p = 0.02). Median values (IQR) of the percentage of NK-lymphocytes were 0.26 [0.2–0.3] in group 1, 0.55 [0.37–0.66] in group 2, and 0.84 [0.35–1.45] in group 3 (p = 0.008). Median values (IQR) of the percentage of T-lymphocytes were 31.9 [31.7–33.1] in group 1, 37.68 [32.15–45.69] in group 2, and 46.9 [37.03–67] in group 3 (p = 0.02). Pair comparisons of groups 2 and 3 also showed significant differences in the percentage of B lymphocytes (p = 0.03) and NK-lymphocytes (p = 0.02).

Conclusions
Significant differences in lymphocyte subpopulations were identified according to the histologic grade of the cecal appendix. More specifically, a lower percentage of B-lymphocytes and a higher percentage of T- and NK-lymphocytes were observed in cases of acute appendicitis. These findings must be confirmed and their etiopathogenic, diagnostic, and prognostic implications elucidated in future studies with larger sample sizes.
Neurological manifestation of HEV infection: still a rare disease entity?
Authors: Wiesenfarth Maximilian , Stamminger Thomas , Zizer Eugen , Tumani Hayrettin , Ludolph Albert C. 
Published: September 2023

AbstractHepatitis E virus (HEV) infection is the most common form of viral hepatitis and is reported to cause neurological manifestation in up to 30% of diagnosed infections. We evaluated the medical reports of all patients (n = 29,994) who were discharged from the Department of Neurology of Ulm University between 01.01.2015 and 30.09.2022 to detect neurological manifestations of HEV. In addition, we retrospectively analyzed the serum samples of n = 99 patients representing different neurological diseases possibly related to HEV for anti-HEV-IgM and anti-HEV-IgG. At the time of discharge from hospital, the etiology of neurological symptoms in these patients was unclear. Overall, five cases of extrahepatic neurological manifestation of HEV (defined as anti-HEV-IgM and HEV-IgG positive) could be detected. An increase of both, anti-IgM- and anti-IgG-serum levels was significantly more common in neuralgic amyotrophy/plexus neuritis/radiculitis than in AIDP/CIDP (P = 0.01), meningitis/encephalitis (P = 0.02), idiopathic peripheral facial paralysis (P = 0.02) and tension headache (P = 0.02). In 15% (n = 15 out of 99) of retrospectively analyzed serum samples, conspicuous positive anti-HEV-IgG levels were detected. This finding was most common in AIDP/CIDP. In conclusion, results of this study indicate neurological manifestation of HEV to be a rare but still underestimated course of disease, occurring at any age and gender. Therefore, testing for HEV should be considered in patients with neurological symptoms of unknown origin, especially in those with neuralgic amyotrophy/plexus neuritis.
The analysis of factors increasing the odds for type 2 diabetes mellitus remission following re-do bariatric surgery after laparoscopic sleeve gastrectomy- cohort study
Authors: Wysocki Michał , Ciszek Karol , Rymarowicz Justyna , Zarzycki Piotr , Walędziak Maciej , Bartosiak Katarzyna , Jaworski Paweł , Kupczyk Wojciech , Szeliga Jacek , Tarnowski Wiesław , Pisarska-Adamczyk Magdalena , Małczak Piotr , Pędziwiatr Michał , Major Piotr ,   PROSS- Collaborative Study Group members, Stefura Tomasz , Myśliwiec Piotr , Hady Hady Razak , Głuszyńska Paulina , Proczko-Stepaniak Monika , Szymański Michał , Janik Michał , Kwiatkowski Andrzej , Materlak Magdalena , Czyżykowski Łukasz , Mawlichanów Maciej , Kowalewski Piotr , Dowgiałło-Gornowicz Natalia , Lech Paweł , Harań Anna , Kowalski Grzegorz , Mulek Rafał , Kreft Michał , Orłowski Michał , Franczak Paula , Binda Artur , Kamiński Mateusz , Pastuszka Maciej , Lisik Wojciech , Szymański Paweł , Katkowski Bartosz , Leśniak Michał 
Published: September 2023

Abstract
Introduction
Metabolic/bariatric surgery is the only proven treatment for type 2 diabetes mellitus (T2D) with curative intent. However, in a number of patients, the surgery is not effective or they may experience a relapse. Those patients can be offered re-do bariatric surgery (RBS).

Purpose
The study aimed to determine factors increasing the odds for T2D remission one year after RBS following primary laparoscopic sleeve gastrectomy.

Methods
A multicenter retrospective cohort study was conducted between January 2010 and January 2020, which included 12 bariatric centers in Poland. The study population was divided into groups: Group 1- patients with T2D remission after RBS (n = 28) and Group 2- patients without T2D remission after RBS (n = 49). T2D remission was defined as HBA1c < 6.0% without glucose-lowering pharmacotherapy and glycemia within normal range at time of follow-up that was completed 12 months after RBS.

Results
Fifty seven females and 20 males were included in the study. Patients who achieved BMI < 33 kg/m2 after RBS and those with %EBMIL > 60.7% had an increased chance of T2D remission (OR = 3.39, 95%CI = 1.28–8.95, p = 0.014 and OR = 12.48, 95%CI 2.67–58.42, p = 0.001, respectively). Time interval between primary LSG and RBS was significantly shorter in Group 1 than in Group 2 [1 (1–4) vs. 3 (2–4) years, p = 0.023].

Conclusions
Shorter time interval between LSG and RBS may ease remission of T2D in case of lack of remission after primary procedure. Significant excess weight loss seems to be the most crucial factor for T2D remission.
Homogenization assumptions for the two-scale analysis of first-order shear deformable shells
Authors: Mester Leonie , Klarmann Simon , Klinkel Sven 
Published: September 2023

AbstractThis contribution presents a multiscale approach for the analysis of shell structures using Reissner–Mindlin kinematics. A distinctive feature is that the thickness of the representative volume element (RVE) corresponds to the shell thickness. The main focus of this paper is on the choice of correct boundary conditions for the RVE. Three different types of boundary conditions, which fulfil the Hill–Mandel condition, are presented to bridge the two scales. A common feature is the application of zero-traction boundary conditions at the top and bottom surfaces of the RVE. Furthermore, an internal constraint is used to reduce the dependency of the stiffness components on the RVE size. The introduced boundary conditions differ mainly in the application of shear strains and their symmetry requirements on the RVE. The characteristic features are compared by means of linear-elastic benchmark tests. It is shown that the stress resultants and tangent stiffness components are obtained correctly. Moreover, the presented approach is verified using different macroscopic shell structures and different mesostructures. Both, linear and nonlinear small strain examples are compared to analytical values or full-scale solutions and demonstrate a wide applicability of the present formulation.
Metabolomic profiling of amino acids study reveals a distinct diagnostic model for diabetic kidney disease
Authors: Wang Jiao , Zhou Chunyu , Zhang Qing , Liu Zhangsuo 
Published: September 2023

AbstractDiabetic kidney disease (DKD), a highly prevalent complication of diabetes mellitus, is a major cause of mortality in patients. However, identifying circulatory markers to diagnose DKD requires a thorough understanding of the metabolic mechanisms of DKD. In this study, we performed ultra-performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) to reveal altered metabolic profiles of amino acids (AAs) in patients with DKD. We found decreased plasma levels of histidine and valine, increased urine levels of proline, decreased urine levels of histidine and valine, and increased saliva levels of arginine in patients with DKD compared with the levels in patients with type 2 diabetes mellitus (T2DM) and in healthy controls. Our analyses of the key metabolites and metabolic enzymes involved in histidine and valine metabolism indicated that the AAs level alterations may be due to enhanced carnosine hydrolysis, decreased degradation of homocarnosine and anserine, enhanced histidine methylation, and systemic enhancement of valine metabolism in patients with DKD. Notably, we generated a distinct diagnostic model with an AUC of 0.957 and an accuracy up to 92.2% on the basis of the AA profiles in plasma, urine and saliva differing in patients with DKD using logistic regression and receiver operating characteristic analyses. In conclusion, our results suggest that altered AA metabolic profiles are associated with the progression of DKD. Our DKD diagnostic model on the basis of AA levels in plasma, urine, and saliva may provide a theoretical basis for innovative strategies to diagnose DKD that may replace cumbersome kidney biopsies.
Investigating projectile penetration into immersed granular beds via CFD-DEM coupling
Authors: Lin Jiayu , Zhao Tao , Jiang Mingjing 
Published: September 2023

Abstract
Projectile penetration into an immersed granular bed is a common phenomenon in both geophysics and engineering, encompassing various scenarios such as immersed crater formation and offshore soil-structure interaction. It involves the complex physical interaction between the fluid and granular materials. In this study, we investigate the dynamics of projectile penetration into a granular bed immersed in a fluid using a coupled computational fluid dynamics and discrete element method (CFD-DEM). The granular bed is composed of polydisperse particles, and the projectile is modeled as a rigid sphere. The morphology of crater formation, the dynamics of the projectile, and the drag force characteristics in immersed cases were studied in detail and compared to the dry scenario. The numerical results show that the final penetration depth of the projectile follows an empirical relation derived from experimental observations, where the falling height and the drag force during penetration obey a power-law function and a modified generalized Poncelet law, respectively. The interstitial fluid not only provides direct drag force, but also enhances the effective drag force of the granular bed by improving its generalized friction and effective viscosity in different configurations. Micro-analyses of the velocity evolution and contact force network in different stages of the fluid–solid interaction were performed to clarify the penetration dynamics. This research provides insights into the mechanisms of projectile penetration and the effects of interstitial fluid on granular media, which are crucial in engineering applications such as offshore anchoring, ball penetration tests in soft sediments, and soil-structure interactions.

Graphical Abstract