bluuebunny/crossref_metadata_2025_split huggingface dataset:
Emergence of Marburg virus: a global perspective on fatal outbreaks and clinical challenges
Authors: Srivastava Shriyansh , Sharma Deepika , Kumar Sachin , Sharma Aditya , Rijal Rishikesh , Asija Ankush , Adhikari Suraj , Rustagi Sarvesh , Sah Sanjit , Al-qaim Zahraa Haleem , Bashyal Prashant , Mohanty Aroop , Barboza Joshuan J. , Rodriguez-Morales Alfonso J. , Sah Ranjit 
Published: September 2023

The Marburg virus (MV), identified in 1967, has caused deadly outbreaks worldwide, the mortality rate of Marburg virus disease (MVD) varies depending on the outbreak and virus strain, but the average case fatality rate is around 50%. However, case fatality rates have varied from 24 to 88% in past outbreaks depending on virus strain and case management. Designated a priority pathogen by the National Institute of Allergy and Infectious Diseases (NIAID), MV induces hemorrhagic fever, organ failure, and coagulation issues in both humans and non-human primates. This review presents an extensive exploration of MVD outbreak evolution, virus structure, and genome, as well as the sources and transmission routes of MV, including human-to-human spread and involvement of natural hosts such as the Egyptian fruit bat (Rousettus aegyptiacus) and other Chiroptera species. The disease progression involves early viral replication impacting immune cells like monocytes, macrophages, and dendritic cells, followed by damage to the spleen, liver, and secondary lymphoid organs. Subsequent spread occurs to hepatocytes, endothelial cells, fibroblasts, and epithelial cells. MV can evade host immune response by inhibiting interferon type I (IFN-1) synthesis. This comprehensive investigation aims to enhance understanding of pathophysiology, cellular tropism, and injury sites in the host, aiding insights into MVD causes. Clinical data and treatments are discussed, albeit current methods to halt MVD outbreaks remain elusive. By elucidating MV infection’s history and mechanisms, this review seeks to advance MV disease treatment, drug development, and vaccine creation. The World Health Organization (WHO) considers MV a high-concern filovirus causing severe and fatal hemorrhagic fever, with a death rate ranging from 24 to 88%. The virus often spreads through contact with infected individuals, originating from animals. Visitors to bat habitats like caves or mines face higher risk. We tailored this search strategy for four databases: Scopus, Web of Science, Google Scholar, and PubMed. we primarily utilized search terms such as “Marburg virus,” “Epidemiology,” “Vaccine,” “Outbreak,” and “Transmission.” To enhance comprehension of the virus and associated disease, this summary offers a comprehensive overview of MV outbreaks, pathophysiology, and management strategies. Continued research and learning hold promise for preventing and controlling future MVD outbreaks.GRAPHICAL ABSTRACT
Specific biomarker mining and rapid detection of Burkholderia cepacia complex by recombinase polymerase amplification
Authors: Fan Yiling , Wang Shujuan , Song Minghui , Zhou Liangliang , Liu Chengzhi , Yang Yan , Yu Shuijing , Yang Meicheng 
Published: September 2023

ObjectiveTo mine specific proteins and their protein-coding genes as suitable molecular biomarkers for the Burkholderia cepacia Complex (BCC) bacteria detection based on mega analysis of microbial proteomic and genomic data comparisons and to develop a real-time recombinase polymerase amplification (rt-RPA) assay for rapid isothermal screening for pharmaceutical and personal care products.MethodsWe constructed an automatic screening framework based on Python to compare the microbial proteomes of 78 BCC strains and 263 non-BCC strains to identify BCC-specific protein sequences. In addition, the specific protein-coding gene and its core DNA sequence were validated in silico with a self-built genome database containing 158 thousand bacteria. The appropriate methodology for BCC detection using rt-RPA was evaluated by 58 strains in pure culture and 33 batches of artificially contaminated pharmaceutical and personal care products.ResultsWe identified the protein SecY and its protein-coding gene secY through the automatic comparison framework. The virtual evaluation of the conserved region of the secY gene showed more than 99.8% specificity from the genome database, and it can distinguish all known BCC species from other bacteria by phylogenetic analysis. Furthermore, the detection limit of the rt-RPA assay targeting the secY gene was 5.6 × 102 CFU of BCC bacteria in pure culture or 1.2 pg of BCC bacteria genomic DNA within 30 min. It was validated to detect &lt;1 CFU/portion of BCC bacteria from artificially contaminated samples after a pre-enrichment process. The relative trueness and sensitivity of the rt-RPA assay were 100% in practice compared to the reference methods.ConclusionThe automatic comparison framework for molecular biomarker mining is straightforward, universal, applicable, and efficient. Based on recognizing the BCC-specific protein SecY and its gene, we successfully established the rt-RPA assay for rapid detection in pharmaceutical and personal care products.
Impact of an online guided physical activity training on cognition and gut-brain axis interactions in older adults: protocol of a randomized controlled trial
Authors: Schrenk Simon J. , Brodoehl Stefan , Flor Stefano , Frahm Christiane , Gaser Christian , Hamdan Rami Abou , Herbsleb Marco , Kaleta Christoph , Kattlun Fabian , Müller Hans-Josef , Puta Christian , Radscheidt Monique , Ruiz-Rizzo Adriana L. , Saraei Tannaz , Scherag André , Steidten Thomas , Witte Otto W. , Finke Kathrin 
Published: September 2023

IntroductionBy 2050, the worldwide percentage of people 65 years and older is assumed to have doubled compared to current numbers. Therefore, finding ways of promoting healthy (cognitive) aging is crucial. Physical activity is considered an effective approach to counteract not only physical but also cognitive decline. However, the underlying mechanisms that drive the benefits of regular physical activity on cognitive function are not fully understood. This randomized controlled trial aims to analyze the effect of an eight-week standardized physical activity training program in older humans on cognitive, brain, and gut-barrier function as well as the relationship between the resulting changes.Methods and analysisOne-hundred healthy participants aged 60 to 75 years will be recruited. First, participants will undergo an extensive baseline assessment consisting of neurocognitive tests, functional and structural brain imaging, physical fitness tests, and gut-microbiome profiling. Next, participants will be randomized into either a multi-component physical activity group (experimental condition) or a relaxation group (active control condition), with each training lasting 8 weeks and including an equal number and duration of exercises. The whole intervention will be online-based, i.e., participants will find their intervention schedule and all materials needed on the study website. After the intervention phase, participants will have their post-intervention assessment, which consists of the same measures and tests as the baseline assessment. The primary outcome of this study is the change in the cognitive parameter of visual processing speed from baseline to post-measurement, which will on average take place 10 weeks after the randomization. Secondary outcomes related to cognitive, brain, and microbiome data will be analyzed exploratory.Clinical trial registration:https://drks.de/search/de/trial/DRKS00028022
A personalized diet intervention improves depression symptoms and changes microbiota and metabolite profiles among community-dwelling older adults
Authors: Magzal Faiga , Turroni Silvia , Fabbrini Marco , Barone Monica , Vitman Schorr Adi , Ofran Ariella , Tamir Snait 
Published: September 2023

IntroductionThe impact of diet on mental well-being and gut microorganisms in humans is well recognized. However, research on the connections between food nutrients, gut microbiota, and mental health remains limited. To address this, the present study aimed to assess the effects of a personalized diet, based on individual needs and aligned with the Mediterranean diet principles, on depression symptoms, quality of life, nutritional intake, and gut microbiota changes among older adults living in the community.MethodsThe intervention involved regular visits from a registered dietitian, who provided tailored dietary recommendations. During the 6-month study, participants completed questionnaires to evaluate their depression levels, quality of life, and dietary habits. Additionally, they provided stool samples for analysis of gut microbiota and metabolites.ResultsThe results demonstrated that the personalized dietary intervention reduced depression symptoms and improved the quality of life among older adults. Furthermore, significant changes in the intake of certain nutrients, such as folate, lutein, zeaxanthin, EPA, and DHA, were observed following the intervention. Moreover, the intervention was associated with increased diversity in the gut microbiome and reduced total short-chain fatty acids, the main metabolites produced by gut microorganisms. The study also revealed correlations between food nutrients, gut microbiota, and mental health parameters.DiscussionIn conclusion, this research highlights the potential advantages of personalized dietary interventions in managing depression and enhancing overall well-being among older populations. It also sheds light on the role of gut microbiota and its metabolites in these effects. The findings offer valuable insights into the significance of nutrition and gut health for mental well-being in older adults.
Educational agents and institutions called into action in suicide prevention, intervention, and postvention
Authors: De Oliveira Janaina Minelli , Dueñas Jorge-Manuel , Morales-Vives Fabia , Gallardo-Nieto Elena 
Published: September 2023

IntroductionSuicide is the second leading cause of death in the 15 to 29 age group worldwide, and is a severe public health problem. Adolescent and young adult individuals attend educational institutions which can play an essential role in detecting and preventing suicide. For this reason, the purpose of this research is to identify what educational institutions and agents are called into action in suicide prevention, intervention, and postvention.MethodsThe method of systematic review of the literature based on the PRISMA protocol was used. The review protocol was registered in PROSPERO (PROSPERO 2020 CRD42020189127). The systematic review yielded 66 articles published between 1990 and February 2023.ResultsThe results show that a wide variety of educational stakeholders are required to intervene for suicide prevention, interventions and postvention between primary education and college. The study describes the different programs that have been provided, the countries in which they have been implemented and the agents who have been targeted. It also identifies gaps in the research on suicide in the educational field.DiscussionOverall, educational suicide initiatives report positive effects on participants’ understanding, attitudes, and beliefs regarding suicide and suicide prevention, although some studies have expressed some caution.
Depletion of tet2 results in age-dependent changes in DNA methylation and gene expression in a zebrafish model of myelodysplastic syndrome
Authors: Neelamraju Yaseswini , Gjini Evisa , Chhangawala Sagar , Fan Hao , He Shuning , Jing Chang-Bin , Nguyen Ashley T. , Prajapati Subhash , Sheridan Caroline , Houvras Yariv , Melnick Ari , Look A. Thomas , Garrett-Bakelman Francine E. 
Published: September 2023

IntroductionMyelodysplastic syndrome (MDS) is a heterogeneous group of clonal hematopoietic disorders characterized by ineffective hematopoiesis, cytopenias, and dysplasia. The gene encoding ten-eleven translocation 2 (tet2), a dioxygenase enzyme that catalyzes the conversion of 5-methylcytosine (5mC) to 5-hydroxymethylcytosine, is a recurrently mutated tumor suppressor gene in MDS and other myeloid malignancies. Previously, we reported a stable zebrafish line with a loss-of-function mutation in the tet2 gene. The tet2m/m-mutant zebrafish developed a pre-MDS state with kidney marrow dysplasia, but normal circulating blood counts by 11 months of age and accompanying anemia, signifying the onset of MDS, by 24 months of age.MethodsIn the current study, we collected progenitor cells from the kidney marrows of the adult tet2m/m and tet2wt/wt fish at 4 and 15 months of age and conducted enhanced reduced representation of bisulfite sequencing (ERRBS) and bulk RNA-seq to measure changes in DNA methylation and gene expression of hematopoietic stem and progenitor cells (HSPCs).Results and discussionA global increase in DNA methylation of gene promoter regions and CpG islands was observed in tet2m/m HSPCs at 4 months of age when compared with the wild type. Furthermore, hypermethylated genes were significantly enriched for targets of SUZ12 and the metal-response-element-binding transcription factor 2 (MTF2)—involved in the polycomb repressive complex 2 (PRC2). However, between 4 and 15 months of age, we observed a paradoxical global decrease in DNA methylation in tet2m/m HSPCs. Gene expression analyses identified upregulation of genes associated with mTORC1 signaling and interferon gamma and alpha responses in tet2m/m HSPCs at 4 months of age when compared with the wild type. Downregulated genes in HSPCs of tet2-mutant fish at 4 months of age were enriched for cell cycle regulation, heme metabolism, and interleukin 2 (IL2)/signal transducer and activator of transcription 5 (STAT5) signaling, possibly related to increased self-renewal and clonal advantage in HSPCs with tet2 loss of function. Finally, there was an overall inverse correlation between overall increased promoter methylation and gene expression.
Математична та імітаційна моделі мікроелектромеханічного акселерометра автономної інерціальної системи визначення просторового положення та місцезнаходження
Authors: Гуриненко Станіслав Олегович 
Published: September 2023

Розглянуто синтезування, створення і перевірка на адекватність та працездатність математичної та імітаційної моделей акселерометра. Запропоновані моделі можуть використовуватися у процесі моделювання та дослідження систем керування рухомим об’єктом, системи визначення просторового положення, місцезнаходження безплатформної інерціальної системи орієнтації та навігації (БІСО або БІНС). Наведено та проаналізовано існуючі математичні моделі акселерометра та описано їх недоліки. У результаті синтезовано та розроблено власні математичну та імітаційну моделі тривісного акселерометра, які можуть бути спрощені до моделей одновісного та двовісного акселерометра. Імітаційна модель розроблена у програмному пакеті Matlab/Simulink, для неї створено керуючу програму, в якій ініціалізуються параметри акселерометра, взяті з технічної документації, та вхідний вплив — прискорення сили тяжіння. Загалом модель містить похибку масштабного коефіцієнта, нелінійну похибку масштабного коефіцієнта, помилки неортогональності, шуми та похибки від впливу перехресних осей. Наведено результат роботи імітаційної моделі під дією сили тяжіння на території Києва із заданими параметрами акселерометра ADXL335 фірми Analog Devices. Працездатність та адекватність розроблених математичної та імітаційної моделей підтверджено порівнянням результатів комп’ютерного моделювання з реальними сигналами, отриманими від акселерометра з урахуванням як типового значення, так і максимального значення параметра чутливості (sensitivity). Розроблена математична модель з відносно великою точністю моделює вихідний сигнал акселерометра та може використовуватися для моделювання, дослідження та опису систем, в яких застосовується акселерометр.
Visual angle enlargement method based on effective reconstruction area
Authors: Wang Qi , Piao Yan , Zhang Hao , Deng Li-Jin 
Published: September 2023

Abstract
The traditional integral imaging system has a small viewing angle, mainly due to the existence of interference pixels in the generated element image. At the same time, in the 3D reconstruction scene, the content that is not in the effective reconstruction area will also cause image crosstalk between adjacent viewpoints, which affects the display angle. In this paper, a viewing angle enlargement method based on an effective reconstruction area is proposed to establish the correspondence between the point pixels in the element image and the micro-lens in the micro-lens array, and the pixel information in the pixel recombination area is derived and updated. The effective reconstruction area is used for constraint, so as to enhance the effective use of the element image information. The experimental results show that the element image with the pixel update has a larger viewing angle and clearer reconstruction content.
PENGARUH KEPEMIMPINAN DAN DISIPLIN KERJA TERHADAP KINERJA KARYAWAN DENGAN VARIABEL INTERVENING BUDAYA ORGANISASI (STUDI PADA PT. MULTI SARANA AGRO MANDIRI) DI LONTAR KECAMATAN PULAU LAUT BARAT KABUPATEN KOTABARU KALIMANTAN SELATAN
Authors: Adam Adam Silvanus 
Published: September 2023

Penelitian ini bertujuan untuk mengetahui dan menganalisis pengaruh Kepemipinan dan Disiplin Kerja terhadap Budaya Organisasi dan Kinerja Karyawan pada PT. Multi Sarana Agro Mandiri. Reponden peneltian berjumlah 90 orang, dengan Teknik sampling proportional random sampling. Instrumen penelitian dengan wawancara, angket, dan dokumentasi. Analisis data menggunakan aplikasi Smart PLS versi 3.0. Hasil penelitian ini disimpulkan bahwa Kepemimpinan berpengaruh positif dan signifikan terhadap Budaya Organisasi; Disiplin Kerja berpengaruh positif dan signifikan terhadap Budaya Organisasi; Kepemimpinan berpengaruh positif dan signifikan terhadap Kinerja Karyawan; Disiplin Kerja berpengaruh positif dan signifikan terhadap Kinerja Karyawan; Budaya Organisasi berpengaruh positif dan signifikan terhadap Kinerja Karyawan; Kepemimpinan berpengaruh signifikan terhadap Kinerja karyawan melalui Budaya Organisasi; dan Disiplin Kerja berpengaruh signifikan terhadap Kinerja Karyawan melalui Budaya Organisasi, pada PT. Multi Sarana Agro Mandiri.
Kata Kunci: Kepemimpinan, Disiplin kerja, Budaya Organisasi, dan Kinerja Karyawan.
ANALISIS PENGARUH KEPEMIMPINAN, SUPERVISI AKADEMIK DAN MOTIVASI KERJA TERHADAP KINERJA GURU MTSN DI KECAMATAN BATANG ALAI SELATAN HULU SUNGAI TENGAH KALIMANTAN SELATAN
Authors: Fajeri Sahibul 
Published: September 2023

Penelitian ini bertujuan untuk menganalisis Pengaruh Kepemimpinan, Supervisi Akademik dan Motivasi Kerja terhadap Kinerja Guru MTsN Di Kecamatan Batang Alai Selatan Hulu Sungai Tengah. Penelitian ini menggunakan metode kuantitatif dengan jenis penelitian korelasional. Populasi penelitian sebanyak 62 orang dan teknik pengambilan sampel dengan teknik jenuh, yaitu keseluruhan populasi sebanyak 62 orang. Teknik pengumpulan data menggunakan kuesioner. Adapun variabel independen terdiri dari Kepemimpinan, Supervisi Akademik dan Motivasi Kerja dan variabel dependen Kinerja Guru. Teknik analisis data menggunakan analisis regresi berganda, dengan uji hipotesis: uji-F untuk simultan dan uji-t untuk parsial serta uji β untuk pengaruh dominan. Hasil penelitian menemukan bahwa variabel kepemimpinan, supervisi akademik dan motivasi kerja berpengaruh signifikan terhadap kinerja guru, baik secara simultan maupun secara parsial. Dari ketiga variabel di atas, variabel yang memiliki pengaruh dominan terhadap kinerja guru adalah variabel kepemimpinan.
Kata Kunci: Kepemimpinan, Supervisi Akademik, Motivasi Kerja dan Kinerja Guru
Gerakan Pembinaan Agama Islam Suku Kokoda di Kampung Warmon
Authors: Muzakki Muhammad , Jaharudin Jaharudin , Santoso Budi 
Published: September 2023

Pendidikan agama Islam merupakan pondasi utama dalam berdirinya agama Islam yang membumi. Pada wilayah Indonesia timur, pembinaan agama Islam masih terbatas pada masjid-masjid raya dan program sosial kemasyarakatan yang letaknya di pusat kota. Padahal pembinaan agama Islam lebih diperlukan bagi masyarakat pesisir dan pedalaman. Mitra masyarakat muslim Warmon Kokoda Kabupaten Sorong adalah masyarakat asli Papua yang telah memeluk Islam karena faktor kepala suku. Meskipun telah lama memeluk agama Islam namun masyarakat muslim Warmon Kokoda minim akan pembinaan agama Islam. Permasalahan utama pada mitra yaitu rendahnya pendidikan agama Islam, rendahnya kemampuan membaca al-Qur’an serta kurangnya kesadaran hidup berorganisasi. Selanjutnya pengabdi akan menggunakan metode konstekstual dalam mengatasi permasalahan mitra, sebab metode ini yang paling tepat dan praktis mengatasi permasalahan yang dialami oleh mitra. Sedangkan gerakan pembinaan agama islam dengan metode kontekstual pada suku Kokoda di kampung Warmon dilakukan dengan cara pembinaan agama Islam pada anak-anak, remaja, dan orang tua. Untuk mempermudah pelaksanaan pengabdian, pengabdi akan melibatkan anggota Ikatan Mahasiswa Muhammadiyah (IMM) Komisariat Dewantara dan Corps Muballigh Muhammadiyah kabupaten Sorong. Adapun hasil dari pengabdian ini berupa stimulus dan pemberian motivasi agar mampu mengurangi permasalahan mitra, selanjutnya pengabdi dan tim akan memantau program yang dilaksanakan agar terus kontinyu sehingga dapat mengatasi permasalahan mitra.
Peran Kepala Sekolah Dalam Pengelolaan Keuangan Pendidikan
Authors: Heriyah N. , Himmah Eloq Faiqoh , Purwanto Eko 
Published: September 2023

Pendidikan mempunyai peranan yang sangat penting dalam peningkatan mutu. Pendidikan diakui sebagai suatu investasi sumber daya manusia. Pendidikan dalam operasionalnya tidak dapat dilepaskan dari masalah dana. Khususnya keuangan sekolah. Kepala sekolah sebagai perencana anggaran sekolah harus memahami pengelolaan keuangan sekolah, terutama dalam pengelolaan dan pemanfaatan semua kebutuhan sekolah. Salah satu unsur penting yang dimiliki oleh suatu sekolah agar menjadi sekolah yang baik adalah dari segi pengelolaan keuangan. Pengelolaan keuangan sekolah sangat penting dalam hubungannya dengan pelaksanaan kegiatan sekolah. Seorang kepala sekolah harus memiliki ilmu pengetahuan tentang pengelolaan sehingga dapat mengelola dengan baik seluruh dana yang masuk untuk kepentingan pendidikan di sekolahnya.