bluuebunny/crossref_metadata_2025_split huggingface dataset:
Plant growth regulators’ role in senescence and quality of lisianthus (Eustoma grandiflorum ‘Mariachi Blue’) cut flowers: Comparison Salicylic acid (SA), Sodium nitroprusside (SNP) and Spermidine (Sp)
Authors: Piri Mohaddeseh , jabbarzadeh zohreh 
Published: May 2023

Abstract
Post-harvest senescence is one of the crucial challenges limiting the commercialization of cut flowers. Long vase life is the most important factor determining the economic value of cut flowers. Major factors limiting the vase life of lisianthus cut flower included sensitivity to ethylene, inability to absorb water resulting in bending and suppression of bud opening and coloring, which must be considered at the post-harvest stage. Therefore, the present study was carried out to investigate three types of growth regulators, salicylic acid (SA), sodium nitroprusside (SNP) and spermidine (Sp), each with four concentrations and in four replicates, on the vase life and quality of Lisianthus cut flowers in post-harvest stage. The three regulators have a positive effect on the longevity of Lisianthus cut flowers and reduce ion leakage, malondialdehyde and hydrogen peroxide and increase the activity of the antioxidant enzymes, ascorbate peroxidase and guaiacol peroxidase, and preserving the degradation of lipid membrane and cell stability. The most appropriate concentrations in this study included 1- and 2-mM salicylic acid and/or spermidine as well as sodium nitroprusside concentrations of 40 and 60 µM.
A comparative experimental study of the efficacy and safety of Quality and Quantity media-cultured mononuclear cell transplantation in ischemic hindlimb mouse model
Authors: Chinchalongporn Wanchai , Chruewkamlow Nuttapol , Sermsathanasawadi Nuttawut , Worateera Kosit , Jintaworn Suthatip , Wongwanit Chumpol , Ruangsetakit Chanean 
Published: May 2023

Abstract
Background: Up to 15-20% of revascularizations for chronic limb-threatening ischemia (CLTI) fail. Peripheral blood mononuclear cell (PBMNC) transplantation is an alternative treatment for CLTI patients, but a meta-analysis study found no significant clinical benefit of this treatment. Impaired function and/or an insufficient number of PBMNCs in CLTI patients might be the cause of unsatisfactory outcomes. Quality and Quantity media-cultured mononuclear cells (QQMNCs) were reported to enhance the number and function of PBMNCs. Accordingly, the aim of this study was to investigate the efficacy and safety of QQMNC transplantation in an ischemic hindlimb mouse model.
Methods: PBMNCs from CLTI patients were cultured in Quality and Quantity (QQ) culture media or standard culture media. In vitro studies, including phenotypic analysis of progenitor cells (CD34+CD133+), M2 macrophages (CD206+), and inactivated T regulatory cells (CD4+CD25+CD127+); colony forming assay; and tube formation assay of QQMNCs and PBMNCs, were conducted. Intramuscular transplantation of QQMNCs or PBMNCs was performed in the ischemic hindlimb mouse model. The clinical appearance of ischemic limbs was observed, and blood flow in ischemic limbs was measured using a laser Doppler perfusion imager. Transplantation outcomes were compared between the QQMNC and PBMNC groups.
Results: Twenty CLTI patients (mean age: 65.9±8.56 years) were included. Diabetes mellitus, hypertension, current smoker status, and chronic kidney disease was found in 12, 17, 11, and 6 patients, respectively. The mean percentages of CD34+ cells, CD133+ cells, CD34+CD133+ progenitor cells, CD206+ cells, colony-forming cells, and tube formation were significantly higher in the QQMNC group than in the PBMNC group. However, the mean percentage of CD4+CD25+CD127+ cells was significantly lower in QQMNCs than in PBMNCs. The colony-forming unit count and Dil-acetylated low-density lipoprotein uptake were both significantly greater in QQMNCs than in PBMNCs. The clinical appearance of post-QQMNC-injected limbs was less severe than the appearance of post-PBMNC-injected limbs. Limb perfusion was significantly better in the QQMNC group than in the PBMNC group.
Conclusions: Proangiogenic and anti-inflammatory phenotypes of MNCs cultured in QQ culture media were successfully reproduced in vitro. Moreover, intramuscular QQMNC transplantation was found to be safe, and resulted in better reperfusion of ischemic mouse hindlimbs compared to PBMNCs.
The synergistic effect of degradation and adsorption for emerging pollutants removal from water and real wastewater by using immobilized activated carbon from pomegranate peels
Authors: Al-sareji Osamah J. , Meiczinger Mónika , Al-Juboori Raed A. , Grmasha Ruqayah Ali , Andredaki Manolia , Somogyi Viola , Idowu Ibijoke A. , Stenger-Kovács Csilla , Jakab Miklós , Lengyel Edina , Hashim Khalid S. 
Published: May 2023

Abstract
In this study, pomegranate peels (PPs) as an abundant fruit processing waste was used to produce cost-effective, eco-friendly, and high-quality activated carbon. The produced carbon (fossil free activated carbon) was used for immobilizing laccase to remove a range of emerging pollutants namely diclofenac, amoxicillin, carbamazepine, and ciprofloxacin from water and wastewater. The loaded activated carbon by laccase (LMPPs) and the unloaded one (MPPs) were characterized using advanced surface chemistry analysis techniques. MPPs was found to have a porous structure with a large surface area and an abundance of acidic functional groups. Laccase immobilization reduced surface area but added active degradation sites. The optimal immobilization parameters were determined as pH 4, 35 oC, and a laccase concentration of 2.5 mg/mL resulting in a 69.8% immobilization yield. The adsorption of the emerging pollutant onto MPPs is best characterized as a spontaneous endothermic process that adheres to the Langmuir isotherm and first-order kinetics. Using synergistic adsorption and enzymatic degradation, the target pollutants (50 mg/L) were eliminated in two hours. In both water types, LMPPs outperformed MPPs. This study shows that pomegranate peels can effectively be harnessed as an enzyme carrier and adsorbent for the removal of emerging pollutants even from a complex sample matrix. The removal of contaminants from wastewater lasted five cycles, whereas it continued up to six cycles for water.
Moxibustion as an adjuvant therapy for heart failure: A systematic review and meta-analysis of a randomized controlled trial
Authors: Gao Bing , Li Lan , Xia Ran , qing-Li Ling , Wang Zhu , Wang Heng , Cheng Yue , Ma Qiang , QI Zhuo Cao , Wang Wei , Zeng Yong Lei , wang Jing 
Published: May 2023

Abstract
Background Moxibustion as an adjuvant therapy shows clinical efficacy in the treatment of heart failure (HF). However, there has been a lack of overall evaluation of the efficacy of moxibustion.Objective To evaluate the efficacy and safety of moxibustion combined with conventional drug therapy, and the quality of evidence regarding its use for preventing ventricular remodeling, improving cardiac function, and improving the prognosis of patients with HF.Method Search the keywords moxibustion therapy and HF from 7 databases. EndNote X8 software was used for literature management. RevMan 5.4.1 software was used to summarize data, and Grading of Recommendation, Assessment, Development, and Evaluation (GRADE) was used to evaluate the quality of research.Results A total of 910 patients were included from 11 studies. Meta-analysis showed that compared with the control groups, patients treated with moxibustion had significantly improved 6-min walk experiment (6-MWD) scores (mean difference [MD] = 57.39, 95% confidence interval [CI] [39.49 to 75.30], P < 0.01) and left ventricular ejection fraction (LVEF) scores (MD = 4.93, 95% CI [3.12 to 6.74] for patients with HF, P < 0.01),According to GRADE, those using LVEF, Lee's HF score, and BNP were “moderate quality” and others were “low quality” or “very low quality.”Conclusion Studies suggest that moxibustion is a safe adjuvant treatment that can improve the cardiac function and prognosis of patients with HF. However, owing to the small sample sizes used in previous studies and the low quality of evidence, these results need to be further verified.
Label-free measurement of antimicrobial peptide interactions with lipid vesicles and nanodiscs using microscale thermophoresis
Authors: Isaksson Johan , Rainsford Philip , Rylandsholm Fredrik G. , Jakubec Martin , Silk Mitchell , Juskewitz Eric , Ericson Johanna U. , Svendsen John Sigurd , Engh Richard A. 
Published: May 2023

Abstract
One strategy to combat antimicrobial resistance is the discovery of new classes of antibiotics. Most antibiotics will at some point interact with the bacterial membrane to either interfere with its integrity or to cross it. Reliable and efficient tools for determining the dissociation constant for membrane binding (KD) and the partitioning coefficient between the aqueous- and membrane phases (KP) are therefore important tools for discovering and optimizing antimicrobial hits.
Here we demonstrate that microscale thermophoresis (MST) can be used for label-free measurement of KD by utilising the intrinsic fluorescence of tryptophan and thereby removing the need for chromophore labelling. As proof of principle, we have used the method to measure the binding of a set of small cyclic AMPs to large unilamellar vesicles (LUVs) and two types of lipid nanodiscs assembled by styrene maleic acid (SMA) and quaternary ammonium SMA (SMA-QA). The measured KD values correlate well with the corresponding measurements using surface plasmon resonance (SPR), also broadly reflecting the tested AMPs’ minimal inhibition concentration (MIC) towards S. aureus and E. coli.
We conclude that MST is a promising method for fast and cost-efficient detection of peptide-lipid interactions.
Impact of Age, Obesity, and Renal Impairment on Outcomes After Autologous Stem Cell Transplantation for Patients with Newly Diagnosed Multiple Myeloma
Authors: Gaffney Kelly , Bakos Jonathan , Velayati Arash , Davis James , Thurlapati Aswani , Weeda Erin , Maldonado Andy , Granger Katelynn , Butcher Coleen , Herrington Taylor , Smith Deidra , Green Kimberly , Hess Brian , Hashmi Hamza 
Published: May 2023

Abstract
Introduction: High-dose chemotherapy-autologous stem cell transplant (HDT-ASCT) improves both depth and duration of remission for patients with transplant-eligible newly diagnosed multiple myeloma (NDMM). Whether certain subgroups of NDMM derive the same benefit from HDT-ASCT remains unclear. We describe our institutional experience highlighting the impact of age, obesity, and renal impairment on outcomes after HDT-ASCT for patients with NDMM in a real-world setting.
Methods: A total of 449 consecutive patients with NDMM treated with HDT-ASCT between February 2012 and August 2022 were included in this retrospective analysis. All patient, disease, and transplant-related data were obtained by chart review of an institutional review board approved myeloma database. Responses to therapy were evaluated per International Myeloma Working Group uniform response criteria. Survival analyses including progression free survival (PFS) and overall survival (OS) were performed using Kaplan-Meier method.
Results: Nearly one-third of the patients (n = 154, 34%) were above the age of 65 at the time of transplant. Forty six percent of the patients had a body mass index (BMI) equal to greater than 30 kg/m2 (n = 205). A vast majority of patients had a normal renal function with 16% (n = 72) having estimated glomerular filtration rate (eGFR) less than 60 ml/min/1.73 m² at the time of transplant. Median follow up for the entire patient population was 29 (1-129) months. When comparing efficacy outcomes across these subgroups, no difference in median PFS was seen for patients with age > 65 (66 versus (vs) 59 months, p=0.46), BMI >30 kg/m2 (91 vs 53 months, p=0.1), or eGFR <60 mL/min/1.73 m2 (50 vs 53 months, p=0.53) when compared to those without these characteristics. Similarly, no difference in median OS was seen for patients with age > 65 (91 vs not reached (NR) months, p=0.69), BMI >30 kg/m2 (NR vs 104 months, p=0.36), or eGFR <60 mL/min/1.73 m2 (NR vs NR months, p=0.25) when compared to those without these characteristics. From a safety standpoint, there were no differences in the incidence of transplant related mortality or secondary malignancy among these subgroups. On multivariable analysis, eGFR less than 60 mL/min/1.73 m2 was the only risk factor significantly associated with superior PFS (hazard ratio 0.581, confidence interval 0.355-0.954).
Conclusion: For patients with NDMM undergoing HDT-ASCT, there is no difference in outcomes based on age, BMI, or renal function, and the presence of one or more of these factors should not preclude patients from HDT-ASCT.
Predictor Bias in Genomic and Phenomic Selection
Authors: Dallinger Hermann Gregor , Löschenberger Franziska , Bistrich Herbert , Ametz Christian , Hetzendorfer Herbert , Michel Sebastian , Buerstmayr Hermann 
Published: May 2023

Abstract
Estimating the breeding value of individuals using genome-wide marker data (genomic prediction) is currently one of the most important drivers of breeding progress in major crops. Recently, remote sensing technologies and aerial hyperspectral imaging of plant canopies combined with a variety of statistical and machine learning models allow prediction of the breeding value of individuals in the absence of genetic marker data. Hyperspectral measurements in the form of near-infrared spectroscopy have been used since the 80s to predict compositional parameters of harvest products, and some recent studies have also used this tool to predict the important parameter grain yield, suggesting that phenomic prediction can outperform genomic prediction. The genome of an individual can be considered static, however gene expression is tissue specific and differs under environmental influences, leading to a tissue and environment specific phenome. Here, we compare genomic prediction and phenomic prediction from hyperspectral measurements of wheat grains for the prediction of a variety of traits including grain yield. We show that phenomic predictions seem equivalent to genomic predictions for some traits in terms of predictive ability. However, phenomic predictions appear heavily biased towards the information present in the predictor. Future studies on this topic should investigate whether population parameters are conserved in phenomic predictions as they are in genomic predictions. Furthermore, we suggest a method to circumvent this issue, which reveals that unbiased phenomic prediction abilities are considerably lower than previously reported.
Calculation of Ultimate Bearing Capacity of Main Joints of Upper Block of Offshore Electrical Platform Based on Secondary Development of SACS
Authors: Li Yan , Yuan JianPing , Yu HuaFeng , Sun Zhen-Zhou , Li XiaoWen , Yan YingBing 
Published: May 2023

Abstract
The structural strength of the upper block of the offshore converter station is very important to the overall structural safety of the offshore converter station. Based on the standardization of the SACS model data file and the result data file, this paper carries out secondary development of the SACS model, and uses MATLAB software to write programs to extract the stress conditions of all nodes under various calculation conditions. According to the BS EN 1993-1-8:2005 standard, the structural strength of all the nodes of the upper block of the offshore converter station was checked. The calculation results show that using MATLAB to write the structural strength check software of offshore converter station can be used to check the strength of all nodes under all working conditions, which can save the calculation time and greatly improve the calculation efficiency. Through the strength check of the developed software, the nodes that do not meet the requirements of the code can be quickly found out, and the structure that does not meet the requirements of the code can be optimized until the requirements of the code are met, so as to ensure the structural safety of the offshore converter station.
Infection status and drug resistance of Klebsiella pneumoniae septicemia in Neonatal Intensive Care Unit
Authors: Ma Hui , Xu JingWen , Zhang YanHong , Zhang RenYan , Wu Jie 
Published: May 2023

Abstract
Background To analyze and evaluate the current status, clinical characteristics, and drug resistance of Klebsiella pneumoniaesepsis infection in Neonatal Intensive Care Unit (NICU), providing effective basis for timely and accurate clinical diagnosis and treatment.Methods Statistical analysis was performed on 75 cases of Enterobacteriaceae septicemia in infants admitted to NICU in a special obstetrics and gynecology hospital in Shanghai from January 2020 to June 2022. Based on bacterial identification, isolates were divided into Klebsiella pneumoniae (KP) group (n = 49) and non-KP Enterobacteriaceae group (n = 26). The infection, clinical characteristics, and bacterial resistance of the two groups of infected patients were compared.Results The increase in levels of platelet, procalcitonin, and CRP in the KP group was more significant than that in the non-KP group. The proportion of antimicrobial drug exposure in the KP group is higher than that in the non KP group. The drug resistance ofKP group to ceftazidime, ceftriaxone, cefepime, ampicillin/sulbactam, aztreonam, ciprofloxacin and compound sulfamethoxazole was significantly higher than that of the non-KP group, whereas the drug resistance rate to cefotetan, gentamycin and to bramycin was significantly lower than that of the non-KP group. 38 cases of Klebsiella pneumoniae producing ESBLs were tested for related resistance genes. The results showed that the main resistance types were SHV and TEM, with detection rates of 60.6% and 28.9%.Conclusions The high infection and drug resistance of Klebsiella pneumoniae have become a serious public health issue; Clinicians should pay attention to differential diagnosis, Reasonable selection of antibiotics to reduce the generation of drug-resistant bacteria.
Droplet digital PCR using HER2/EIF2C1 ratio for detection of HER2 amplification in FFPE breast cancer tissues with low or equivocal HER2 expression
Authors: Tantiwetrueangdet Anchalee , Panvichian Ravat , Wongwaisayawan Sansanee , Phornsarayuth Pitichai , Lertsithichai Panuwat 
Published: May 2023

Abstract
HER2 amplification/overexpression is the predictive biomarker for HER2-targeted therapy. However, there are technical limitations to HER2 detection by fluorescence in situ hybridization (FISH) and immunohistochemistry (IHC). Recently, novel HER2-directed antibody-drug conjugates (ADCs) have shown benefits in HER2-negative breast cancer (BC) with low HER2 expression (i.e., IHC1+ or IHC2+ with FISH-negative). We investigated whether droplet digital PCR (ddPCR) using the HER2/EIF2C1 ratio could be an alternative HER2 detection assay in formalin-fixed, paraffin-embedded (FFPE) BC tissues with low or equivocal HER2 expression. We determined HER2 status by ddPCR in 150 FFPE BC tissues previously classified as IHC1+, IHC2+, and IHC3+; 90 of these were previously determined as FISH-negative and FISH-positive. Optimal cutoff thresholds for the HER2/EIF2C1 ratio, determined by the receiver operating characteristics (ROC) curve, were 2.72 (98% sensitivity, 88% specificity) and 2.64 (89.23% sensitivity, 92% specificity) using IHC and FISH as standard methods, respectively. The concordance rate of HER2 status (n=144) determined by IHC/FISH and ddPCR was 89.58% (kappa=0.791, 89.85% sensitivity, 89.33% specificity). The HER2/EIF2C1 ratio in the IHC3+ group was significantly higher than in IHC1+ and IHC2+ groups (P<0.0001). In IHC3+, the concordance between IHC/FISH and ddPCR was 98% (kappa=1.00). In IHC2+ (n=44), the concordance between FISH and ddPCR was 79.54% (kappa=0.579, 65% sensitivity, 91.7% specificity); the HER2/EIF2C1 ratio in FISH-positive cases was significantly higher than in FISH-negative cases (P<0.001). Interestingly, 12% of IHC1+ cases showed HER2 amplification by ddPCR. Thus, ddPCR using the HER2/EIF2C1 ratio should be an alternative HER2 detection assay in FFPE BC tissues, even with low or equivocal HER2 expression.
Listening and Learning: a qualitative study of Scottish care home staff experiences of managing COVID-19 between March 2020-August 2022
Authors: Burton Jennifer Kirsty , Drummond Maria , Gallacher Katie I , Quinn Terence J 
Published: May 2023

Abstract
Background The serious outcomes of outbreaks of COVID-19 in care homes have been described internationally. The experiences of professionals working through outbreaks has received less attention, missing opportunities to acknowledge and learn lessons. Our aim was to explore the experiences of care home staff in Scotland of managing COVID-19 within their homes to help inform understanding and future practice.Methods From April to August 2022, 34 individual semi-structured interviews were conducted with care home staff working in homes which experienced an outbreak(s) of COVID-19. Reflexive thematic methods were used to analyse verbatim deidentified transcripts.Findings: There was no singular experience of COVID-19 outbreaks within care homes. We identified four broad groupings of homes with outbreaks (significant outbreaks, managed outbreaks, outbreaks in remote/rural homes & outbreaks in homes supporting younger adults), with overlaps in timing and severity and variation in the support received and impact. The national response to the COVID-19 pandemic resulted in fundamental change to care home relationships. Staff responded by adaptation in uncertainty. However, they were challenged by emerging inequalities. There were tensions between staff experience and evolving external approaches to regulation and oversight. All this change resulted in psychological impacts on staff. However, there was also widespread evidence of compassionate leadership and teamwork in their responses. Effective sources of support were underpinned by respectful relationships and continuity, tailored to individual contexts.Conclusions The lived experiences of care home staff during the COVID-19 pandemic provide valuable insights applicable beyond the pandemic context. This includes: recognition of the specialism, complexity and diversity of care home practice; the value afforded by embedding genuine representation and involvement in planning, policy-making and research; the need for individualising to individuals in their contexts and the value of fostering respectful relationships across professional groups to support residents.