bluuebunny/crossref_metadata_2025_split huggingface dataset:
Effectiveness and user experience of nose and throat swabbing techniques for SARS-CoV-2 detection: results from the UK COVID-19 National Testing Programme
Authors: Futschik Matthias E. , Kulasegaran-Shylini Raghavendran , Blandford Edward , Harper Sean , Chapman David , Turek Elena , Agrawal Somya , Phillips Valerie , Fordham Hannah , Chan Lee , Kidd Mike , Dodgson Andrew , Klapper Paul E. , Sudhanva Malur , Vipond Richard , Hopkins Susan , Peto Tim , Tunkel Sarah , Fowler Tom 
Published: January 2025

Abstract

Background
The UK’s National Health Service Test and Trace (NHSTT) program aimed to provide the most effective and accessible SARS-CoV-2 testing approach possible. Early user feedback indicated that there were accessibility issues associated with throat swabbing. We report the results of service evaluations performed by NHSTT to assess the effectiveness and user acceptance of swabbing approaches, as well as qualitative findings of user experiences from research reports, surveys, and incident reports. Our intent is to present and summarize our findings about the application of alternative swabbing approaches during the COVID-19 pandemic in the UK.

Methods
From May 2020 to December 2021, NHSTT conducted a series of service evaluations assessing self-swabbing and assisted swabbing of the nose and throat, and nose only (anterior nares/mid-turbinate) using polymerase chain reaction (PCR) and lateral flow devices (LFDs), for diagnostic suitability within the COVID-19 National Testing Programme. Outcomes included observational user feedback on swabbing approaches and quantitative testing performance (concordance, sensitivity, and specificity). A post-hoc indirect comparison of swabbing approaches was also performed. Additionally, an analysis of existing cross-service research was conducted in April 2021 to determine user feedback regarding swabbing approaches.

Results
Observational data from cross-service research indicated a user preference for nose swabbing over throat swabbing. Significantly more users reported that nose swabbing was easier to perform than throat swabbing (50% vs. 12%) and there were significantly fewer reported incidents. In the service evaluations, while there was reduced sensitivity for nose-only swabbing for PCR (88%) compared with nose and throat swabbing, similar sensitivities were observed for nose-only and nose and throat swabbing for LFDs. The sensitivity of nose-only swabbing for LFDs was higher for individuals with higher viral concentrations.

Conclusions
User experience analyses supported a preference for nose-only swabbing. Nose-only swabbing for LFDs provided sufficient diagnostic accuracy, supporting its use as a viable option in the COVID-19 National Testing Programme. Less invasive swabbing approaches are important to maximize testing accessibility and alongside other behavioral interventions, increase user uptake.
Identification of βIIΣ1-Spectrin as a Binding Partner of the GH-regulated Human Obesity Scaffold Protein SH2B1
Authors: Lanning Nathan J , Mancour Liliya , Argetsinger Lawrence S , Archer Stephen , Carter-Su Christin 
Published: January 2025

Abstract
SH2B1β is a multifunctional scaffold protein that modulates cytoskeletal processes such as cellular motility and neurite outgrowth. To identify novel SH2B1β-interacting proteins involved in these processes, a yeast 2-hybrid assay was performed. The C-terminal 159 residues of the cytoskeleton structural protein, βIIΣ1-spectrin, interacted with the N-terminal 260 residues of SH2B1β, a region implicated in SH2B1β enhancement of cell motility and localization at the plasma membrane. The interaction between SH2B1β and βIIΣ1-spectrin (2205-2363) requires residues 1 through 150 in SH2B1β, with residues 105 through 120 playing a key role. While βIIΣ1-spectrin (2205-2363) was expressed throughout the cell, it colocalized with SH2B1β when coexpressed with SH2B1β mutants with varied intracellular localizations. The SH2B1β-βIIΣ1-spectrin (2205-2363) interaction impaired the ability of SH2B1β to enter the nucleus. A slightly larger βIIΣ1-spectrin fragment (2170-2363) with an intact pleckstrin homology domain localized primarily to the plasma membrane and cytoplasm, similar to SH2B1β. Similarly, full-length βIIΣ1-spectrin colocalized at the plasma membrane and cytoplasm with SH2B1β as well as the SH2B1β-regulated tyrosine kinase, JAK2. Phosphorylation of spectrins has been shown to regulate their localization and function. Coexpression of βIIΣ1-spectrin, JAK2, and SH2B1β resulted in SH2B1β-dependent tyrosyl phosphorylation of βIIΣ1-spectrin. Finally, stimulation with GH induced formation of an endogenous complex containing βII-spectrin, SH2B1, and JAK2 in 3T3-F442A cells and increased tyrosyl phosphorylation of βII-spectrin. Our results identify a novel interaction between SH2B1β, βIIΣ1-spectrin, and JAK2 resulting in JAK2- and SHB1-dependent tyrosyl phosphorylation of βII-spectrin. This raises the possibility that the many other ligand-activated tyrosine kinases that signal through SH2B1 form similar complexes with βIIΣ1-spectrin.
Multidisciplinary management of a patient with acute oesophageal necrosis and severe neurological symptoms with full recovery
Authors: Terehova Katrīna , Zaļizko Poļina , Lazdovska Krista , Skumbiņš Raimonds , Puķītis Aldis 
Published: January 2025

Background: Acute oesophageal necrosis is a rare and poorly understood disorder of the oesophagus. It poses significant diagnostic and therapeutic challenges as it often presents with multiple comorbidities and a poor prognosis. Case report: We report a case of 42-year-old male with no significant medical history, who presented to our clinic due to neurological deficits and repeated vomiting for 2 weeks. He initially denied alcohol abuse. Upper endoscopy demonstrated circular black discoloration of the distal oesophagus. The patient was treated with proton pump inhibitors and received treatment for his comorbidities. Esophagogastroduodenoscopy was performed to assess improvement. The patient’s symptoms gradually resolved. Conclusion: This case is one of the few to show an association between alcohol abuse and acute oesophageal necrosis and underscores the importance of timely diagnosis and multidisciplinary treatment in patient with fully recovered condition.
Novel digital leakage notification system can promote better stoma management routines: a multicentre clinical trial
Authors: Brady Richard RW , Sheard Diane , Alty Mandie , Vestergaard Martin , Boisen Esben Bo , Ainsworth Rachel , Hansen Helle Doré , Ajslev Teresa Adeltoft 
Published: January 2025

Background: Most people with a stoma are anxious about stoma-related leakage. Aims: To investigate the impact of a novel digital leakage notification system on worry related to stoma leakage, and to evaluate the effect on overall stoma care management. Method: A 12-week interventional, single-arm, multicentre study was conducted in the UK to evaluate the novel digital leakage notification system, including a telemedicine-based support service (=test product), as part of routine stoma care in patients with a recent stoma formation (ClinicalTrials.gov: NCT05135754). Patients completed questionnaires at baseline and at other time points throughout the study period. Study nurses evaluated the test product on individual patient completion of the trial. Findings: The intention-to-treat-population consisted of 92 patients. At baseline, 39% of patients worried to a high or very high degree about leakage, which fell to 14% at the final evaluation (P<0.001), at which point patients also reported significantly fewer baseplate changes due to leakage-related worry (P<0.001). The vast majority of nurses reported that the test product helped patients (82%) improve their stoma-care routines (P<0.0001). Conclusion: The use of a digital leakage notification system reduced leakage-related worry and improved stoma self-care.
Cloud Enterprise Systems – State of the Art und Herausforderungen für Unternehmen
Authors: Schütte Reinhard , Kari Mohamed 
Published: January 2025

Zusammenfassung
Die Softwarehersteller propagieren für Enterprise Systems das Cloud Computing. So sollen sämtliche Komponenten von Enterprise Systems langfristig nur noch als Software as a Service genutzt werden. Aufgrund der Abhängigkeit vieler Anwendungsunternehmen von den wenigen Anbietern (Microsoft, SAP, Oracle, Infor, Sage, Salesforce etc.) stellt sich die Frage, welchen Entwicklungen die Applikationsarchitekturen und IT-Prozesse unterworfen sein werden. Ausgehend von einer Entfaltung dessen, was unter Enterprise Systems verstanden wird und welche Präsuppositionen den nachfolgenden Abschnitten unterliegen, wird der State of the Art heutiger Enterprise Systems kurz skizziert. Es wird eine Referenzarchitektur entworfen, in der die Services der drei großen Hyperscaler verortet werden. Zuletzt werden die strategischen, prozessualen und applikationsmäßigen sowie systemtechnischen Implikationen des Cloud Computing für die Anwendungsunternehmen aufgezeigt. Der Beitrag verdeutlicht, dass die technologischen Paradigmen und Systems-Engineering-Vorgehensweisen im Cloud Computing die zukünftige Einführung von Enterprise Systems und auch deren Betrieb nachhaltig verändern werden. Der Beitrag schließt mit einem Ausblick auf zukünftige Herausforderungen für die Forschung und die betriebliche Praxis.
July effect in hospitalized cirrhosis patients: A US nationwide study using difference-in-differences analysis
Authors: Celdir Melis Gokce , Wehby George , Prakash Shahana , Tanaka Tomohiro 
Published: January 2025

Background
The July effect in US teaching hospitals has been studied with conflicting results. We aimed to evaluate the effect of physician turnover in July on the clinical outcomes of patients hospitalized with cirrhosis.

Methods
We utilized the Nationwide Inpatient Sample database (2016–2019) to identify patients hospitalized with cirrhosis and liver-related complications (variceal bleeding, hepatorenal syndrome, acute-on-chronic liver failure). We used difference-in-differences analysis to compare teaching and non-teaching hospital differences in mortality and length of stay (LOS) in May and July, and trends in outcomes in other months before and after July.

Results
We included 78,371 hospitalizations in teaching and 23,518 in non-teaching hospitals in May and July. Teaching hospital admissions had overall higher complication rates and mortality compared to non-teaching hospitals. We did not find a difference in mortality between teaching and non-teaching hospitals in all cirrhotic patients (adjusted odds ratio 1.01, 95%CI [0.88–1.16]) or in those with severe complications (0.87, [0.72–1.06]). There was greater LOS in July vs. May in teaching hospitals relative to non-teaching hospitals for all patients with cirrhosis (adjusted rate ratio 1.03, 95%CI [1.02–1.05]) and for those with severe complications (1.19, [1.17–1.21]). The months after July were associated with longer LOS in teaching hospitals, with the effect gradually diminishing over the subsequent months.

Conclusions
Our study suggests trainee turnover in July did not affect mortality, but lengthened hospital stays for patients with cirrhosis, highlighting the need for effective supervision of new trainees and strategies to mitigate operational disruptions for improved clinical management.
As contribuições do Programa de Integração Ensino e Extensão (PEEX) na formação de estudantes de graduação da Universidade Federal do Cariri (UFCA)
Authors: Sousa Angélica Almeida de , Brandão Pamela de Medeiros 
Published: January 2025

Este artigo analisa as contribuições do Programa de Integração Ensino e Extensão (PEEX) para a formação de estudantes dos cursos de graduação da Universidade Federal do Cariri (UFCA) no período de 2016 a 2019. Para tanto, inicialmente apresenta-se um panorama sobre o modelo de gestão do PEEX, bem como o mapeamento dos seus projetos, atividades e seus participantes. E verifica-se o nível de integração entre as atividades de ensino e de extensão realizadas no âmbito de disciplinas dos cursos de graduação por meio do Programa, e identifica-se as suas contribuições para o desenvolvimento de competências e habilidades dos estudantes, bolsistas e voluntários do projeto. Trata-se de um estudo exploratório-descritivo que adotou uma abordagem qualiquantitativa e utilizou o estudo de caso como estratégia de pesquisa, realizando um levantamento (Survey) e pesquisa documental para coleta dos dados que foram analisados por meio das técnicas de análise do conteúdo e de análise estatística descritiva básica. A partir da pesquisa realizada, avalia-se que o PEEX tem contribuído satisfatoriamente para a formação de estudantes dos cursos de graduação da UFCA, na medida em que permitiu o desenvolvimento de competências e habilidades dos estudantes bolsistas e voluntários do projeto.
Konsulting historyczny gier analogowych w Polsce i w kontekście
Authors: Szeja Jerzy , Mochocki Michał 
Published: January 2025

Jest to czwarty z serii artykułów powstałych w projekcie Konsulting historyczny gier Polskiego Towarzystwa Badania Gier, realizowanego w ramach grantu z CRPK. Traktuje o praktykach i typowych umiejętnościach konsultantów historycznych przy grach analogowych, szczególnie planszowych. Pierwsza połowa tekstu to przegląd stanu wiedzy o historycznym konsultingu gier niecyfrowych na rynku międzynarodowym według informacji dostępnych w (raczej nielicznych) publikacjach naukowych oraz w oficjalnych materiałach publikowanych przez wydawców i twórców. Druga połowa tekstu to podsumowanie pogłębionych wywiadów, jakie przeprowadziliśmy z profesjonalistami, którzy pracowali jako konsultanci historyczni lub we współpracy z konsultantami przy tworzeniu gier o tematyce historycznej na rynku polskim.
Role of circRMRP and circRPL27 in chronic obstructive pulmonary disease
Authors: Li JianFang , Zhang PengFei , Zeng XianJing , Liu Rong 
Published: January 2025

Abstract
Chronic obstructive pulmonary disease (COPD) is one of the leading causes of death and disability worldwide, and circRNA dysregulation is functionally associated with COPD. This study explored the potential of circRMRP and circRPL27 as biomarkers of COPD. Blood samples from COPD patients and healthy controls were collected. COPD patients were divided into mild, moderate, and severe groups according to lung function. Quantitative real-time polymerase chain reaction technology was used to determine the expression of circRPL27 and circRMRP in COPD. Receiver operating characteristic curve was drawn to explore the value of circRMRP and circRPL27 in diagnosing COPD. circRMRP and circRPL27 levels were elevated in serum of COPD patients and increased with the severity of COPD. CircRMRP and circRPL27 were associated with smoking history, WBC, and FEV1/FVC, and were positively correlated with smoking history and WBC, and negatively correlated with FEV1/FVC. In COPD, both circRMRP and circRPL27 had diagnostic values, but circRPL27 was better. circRMRP and circRPL27 may be useful non-invasive biomarkers for COPD diagnosis.
BA inhibits LPS-stimulated inflammatory response and apoptosis in human middle ear epithelial cells by regulating the Nf-Kb/Iκbα axis
Authors: He Qian , Cai Yanzhi , Kong Meihua 
Published: January 2025

Abstract
Otitis media (OM) is a prevalent childhood ear disease characterized by inflammation of the middle ear cavity, which can lead to ear pain, fever, and hearing loss. The pathogenesis of OM is multifaceted, encompassing a variety of factors including bacterial or viral infections, host immune responses, and the function of middle ear epithelial cells. Boswellic acid (BA), a natural triterpene compound extracted from frankincense resin, has been proven to possess significant anti-inflammatory and immunomodulatory effects. This study aims to investigate the effects of BA on lipopolysaccharide (LPS)-stimulated inflammatory responses and apoptosis in human middle ear epithelial cells (HMEECs) and its potential mechanisms. Our findings demonstrated that BA enhances the proliferation of LPS-stimulated HMEECs and concurrently inhibits their apoptosis. In addition, BA blocked LPS-stimulated HMEEC inflammation. Mechanistically, BA suppressed the NF-κB/IκBα axis in LPS-stimulated HMEECs. In conclusion, BA effectively inhibits LPS-stimulated inflammation and apoptosis by mediating the NF-κB/IκBα axis, highlighting its potential as a therapeutic agent for OM.
Characterization of phenolic compounds and evaluation of anti-diabetic potential in Cannabis sativa L. seeds: In vivo, in vitro, and in silico studies
Authors: El-Mernissi Rafik , El Menyiy Naoual , Metouekel Amira , Zouhri Aziz , El-Mernissi Yahya , Siddique Farhan , Nadeem Sumaira , Amhamdi Hassan , Abboussi Oualid , Alsahli Abdulaziz Abdullah , Bourhia Mohammed , Dauelbait Musaab , Shazly Gamal A. , Hajji Lhoussain 
Published: January 2025

Abstract
Moroccan Cannabis sativa L. seeds were investigated for their phenolic profile and antidiabetic potential. Ultra-high-performance liquid chromatography with diode array detection and electrospray ionization mass spectrometry analysis revealed a rich phenolic composition, including benzoic acid, cannabisin B, genistein, and epicatechin. In vitro, the seed extract exhibited potent α-amylase inhibitory activity (half-maximal inhibitory concentration = 25.02 ± 4.03 μg/mL). In vivo studies in diabetic rats demonstrated significant hypoglycemic, hypolipidemic, hepatoprotective, and nephroprotective effects. Molecular docking studies further supported these findings, revealing strong interactions between identified phenolic and the α-amylase enzyme. These results highlight the potential of C. sativa seeds as a natural source of bioactive compounds for diabetes management.
On the generalized fractional Laplace–Bessel operator
Authors: Bouzeffour Fethi 
Published: January 2025

Abstract
In this paper, we investigate the fractional powers of the Laplace–Bessel operator

Δ
ν

{\Delta_{\nu}}

defined on the positive space

ℝ
+
n

{\mathbb{R}^{n}_{+}}

:

Δ
ν

=

∑

i
=
1

n

∂
2

∂
⁡

x
i
2

+

ν
i

x
i

⁢

∂

∂
⁡

x
i

,

ν
i

≥
0

.

\Delta_{\nu}=\sum_{i=1}^{n}\frac{\partial^{2}}{\partial x_{i}^{2}}+\frac{\nu_{%
i}}{x_{i}}\frac{\partial}{\partial x_{i}},\quad\nu_{i}\geq 0.

Initially, we establish a Pizzetti-type formula for the Laplace–Bessel operator. We then advance our investigation to explore the fractional powers of this operator, developing a new normalized pointwise formula for the fractional operator

(

-

Δ
ν

)

s

{(-\Delta_{\nu})^{s}}

within the Schwartz space, specifically valid for all

s
>
0

{s>0}

,

s
≠

1
,
2
,
…

s\neq 1,2,\dots

.
Humans as Donations and the Question of Temple Slavery in Early Mesopotamia
Authors: Bartash Vitali 
Published: January 2025

Abstract
This paper examines the practice of donating people to deities in southern Mesopotamia between 2600 and 2000 BC. It offers a diachronic analysis of Early Dynastic, Old Akkadian, and Ur III sources and considers Old and Neo-Babylonian evidence to address questions raised in Gelb’s (1972. “The Arua Institution.” RA 66: 1–32) foundational study. This paper demonstrates, first, that donors and recipients were rarely close relatives, as the dedicatees often included foreign captives, former slaves, orphans, and incomplete families temporarily under patronage prior to donation. Secondly, despite becoming temple “servants” (performing mostly unskilled labor like water-drawing, weaving, and, rarely, cult-related tasks), officials recorded the names of the donors of many dedicatees. In some cases, this was because the dedicatees were former slaves who continued living with their former masters after donation (and manumission), supporting them in old age as part of the paramonē arrangement. Other dedicatees may have lived with temple officials and employees or even within temple complexes. Third, the paper challenges the “temple slaves” model in interpreting the status of dedicatees in early Mesopotamia. Although third-millennium sources describe them as a deity’s “servants,” similar to how slaves in private households were referred to as someone’s “servants,” the dedicatees faced a social and legal dead end: temple officials could neither sell nor release them because their master or mistress was a deity. In effect, third-millennium dedicatees became part of a broader “menial” class of full-time temple dependents. Their status resembled that of the Neo-Babylonian oblates (širkū), who were legally free but subject to strict temple control. Finally, building on Patterson’s (1982. Slavery and Social Death. A Comparative Study. Cambridge, MA, London: Harvard University Press) model of slavery as a substitute for death, this paper argues that the donation of humans to temples in southern Mesopotamia functioned as an alternative to slavery. For the local impoverished population, donating family members to temples provided an option other than outright sale into slavery. Additionally, donating foreign captives to temples served as a more effective method of control and integration than enslavement.
Unveiling novel biomarkers for platinum chemoresistance in ovarian cancer
Authors: Wang Caixia , Peng Changsheng , Xie Chuan 
Published: January 2025

Abstract
Primary chemoresistance to platinum-based treatment is observed in approximately 33% of individuals diagnosed with ovarian cancer; however, conventional clinical markers exhibit limited predictive value for chemoresistance. This study aimed to discover new genetic markers that can predict primary resistance to platinum-based chemotherapy. Through the analysis of three GEO datasets (GSE114206, GSE51373, and GSE63885) utilizing bioinformatics methodologies, we identified two specific genes, MFAP4 and EFEMP1. The findings revealed that the areas under the receiver operating characteristic curves for MFAP4 and EFEMP1 were 0.716 and 0.657 in the training cohort, and 0.629 and 0.746 in the testing cohort, respectively. In all cases or in cases treated with platin, high expression of MFAP4 and EFEMP1 was linked to shortened overall survival and progression-free survival. MFAP4 and EFEMP1 were positively correlated with epithelial–mesenchymal transition, TGF-β signaling, KRAS signaling, and so on. The high expression groups of MFAP4 and EFEMP1 exhibited elevated stromal, immune, and ESTIMATE scores. Finally, we constructed a regulatory network involving lncRNA–miRNA–mRNA interactions. In summary, MFAP4 and EFEMP1 have the potential to serve as predictive indicators for both response to platinum-based chemotherapy and survival rates, and might be regarded as innovative biomarkers and therapeutic targets for OC patients.
Equiatomic transition metal (T) silicides TT′Si: systematics of 29Si NMR Knight shifts
Authors: Benndorf Christopher , Eckert Hellmut , Kösters Jutta , Pöttgen Rainer 
Published: January 2025

Abstract
A series of 64 ternary equiatomic tetrelides TT′Tt (T = electron-poor transition metal; T′ = electron-rich transition metal; Tt = Si, Ge) was synthesized by arc-melting and subsequent annealing. The samples were characterized by powder X-ray diffraction and 20 structures were refined from single-crystal X-ray diffractometer data. These tetrelides (mostly silicides) crystallize with TiNiSi-related structures, orthorhombic space groups Pnma. They are ternary ordered, orthorhombically distorted superstructure variants of the aristotype AlB2. The precise single-crystal data show distinct differences in the bond lengths and angles within the three-dimensional [T′Si] networks as a consequence of different electron densities driven by the electronegativity differences. Systematic solid-state NMR-spectroscopic investigations allow observing the effect of the two transition metal atoms upon the 29Si isotropic resonance shifts. For the silicides featuring T′ elements from groups IX and X this value can be predicted from element-specific increments.