bluuebunny/crossref_metadata_2025_split huggingface dataset:
Effects of Salinity Beyond Coalescence on Submicron Aerosol Distributions
Authors: Dubitsky L. , Stokes M. D. , Deane G. B. , Bird J. C. 
Published: May 2023

AbstractBubbles entrained by ocean waves rise to the surface and burst, creating a shower of droplets which contribute to sea spray aerosols. Submicron‐sized droplets, of which an estimated 60%–80% come from a bursting bubble film cap, play a key role in global climate atmospheric processes. However, many aspects of predicting the number and size of submicron drops emitted from a bursting bubble remain unknown. It is well‐documented that higher salinity increases submicron droplet production, which has been attributed to the role of salt in the suppression of bubble coalescence. We experimentally show that submicron drop production increases with salinity despite using a salt that does not affect bubble coalescence, indicating that salinity plays a role in the physics of submicron aerosol formation beyond coalescence. Laboratory experiments are conducted using sodium acetate solutions of salinity S = 0.001–0.1 M with millimeter‐sized bubbles generated via a needle. Unlike previous studies, the measured droplet size distributions are converted to formation diameter, revealing that the peak aerosol formation diameter decreases with higher salinity. Applying this diameter conversion to past studies, we find the peak formation diameter exhibits a scaling of Dform ∼ S−0.32 across three orders of magnitude in salinity and for a variety of salts, bubble coalescence behaviors, and bubble generation mechanisms. This result suggests that salinity has a systematic effect on the length scale of the rupturing bubble film which generates the aerosols. Consequently, salinity likely impacts the submicron aerosol production in oceanic environments even if bubble coalescence is negligible.
Effects of Circulation on Tropical Cloud Feedbacks in High‐Resolution Simulations
Authors: Mackie Anna , Byrne Michael P. 
Published: May 2023

AbstractUncertainty in the response of clouds to global warming remains a significant barrier to reducing uncertainty in climate sensitivity. A key question is the extent to which the dynamic component—that which is due to changes in circulation rather than changes in the thermodynamic properties of clouds—contributes to the total cloud feedback. Here, simulations with a range of cloud‐resolving models are analyzed to quantify the impact of circulation changes on tropical cloud feedbacks. The dynamic component of the cloud feedback is substantial for some models and is controlled both by sea surface temperature (SST) induced changes in circulation and nonlinearity in the climatological relationship between clouds and circulation. We find notable inter‐model differences in the extent to which ascending regions narrow or expand in response to a change in SST, which we link to differences in the longwave and shortwave dynamic components across models. The diversity of changes in ascent area is coupled to intermodel differences in non‐radiative diabatic heating in ascending regions.
Middle Jurassic Paleolatitude of the Tethyan Himalaya: New Insights Into the Evolution of the Neo‐Tethys Ocean
Authors: Jiao Xianwei , Yang Tianshui , Bian Weiwei , Wang Suo , Ma Jiahui , Peng Wenxiao , Zhang Shihong , Wu Huaichun , Li Haiyan 
Published: May 2023

AbstractThe paleogeography of the Tethyan Himalaya (TH) during the Mesozoic is vital to constrain the evolutionary history of the Neo‐Tethys Ocean, but reliable paleomagnetic data from Jurassic rocks in this area are scarce. Here, we report the first high‐quality paleomagnetic results from the Middle Jurassic (∼175–173 Ma) Lanongla Formation limestones in the Nyalam area of the TH. For most specimens, stepwise thermal or hybrid demagnetization reveals two well‐defined magnetization components. A low‐temperature component, which is isolated between natural remanent magnetization and 200–300°C, is consistent with the present geomagnetic field direction. A high‐temperature component, which is isolated between 300–350°C/10–25 mT and 500–580°C/60–120 mT, passes fold tests at the 95% and 99% confidence level, indicating a prefolding primary magnetization. The tilt‐corrected site‐mean direction for 28 paleomagnetic sites is Ds = 331.0° and Is = −49.9° with α95 = 2.7°, which provides a Fisherian site‐mean paleopole at 23.7°N, 292.9°E with A95 = 2.8° and a paleolatitude of 31.7 ± 2.8°S for the Nyalam study area (28.6°N, 86.1°E). A comparison between the reliable Middle Jurassic‐Early Cretaceous poles obtained from the TH and those observed from the Lhasa terrane reveals that the Neo‐Tethys Ocean for the reference point (29.1°N, 86.1°E) had a latitudinal width of 3,500 ± 1,000 km at ∼174 Ma, reached its greatest width of 7,000 ± 1,000 km at ∼137 Ma, and had an average latitudinal spreading rate of ∼10.4 cm/year during ∼174–137 Ma.
Polarization-sensitive intensity diffraction tomography
Authors: Song Seungri , Kim Jeongsoo , Moon Taegyun , Seong Baekcheon , Kim Woovin , Yoo Chang-Hyuk , Choi Jun-Kyu , Joo Chulmin 
Published: May 2023

AbstractOptical anisotropy, which is an intrinsic property of many materials, originates from the structural arrangement of molecular structures, and to date, various polarization-sensitive imaging (PSI) methods have been developed to investigate the nature of anisotropic materials. In particular, the recently developed tomographic PSI technologies enable the investigation of anisotropic materials through volumetric mappings of the anisotropy distribution of these materials. However, these reported methods mostly operate on a single scattering model, and are thus not suitable for three-dimensional (3D) PSI imaging of multiple scattering samples. Here, we present a novel reference-free 3D polarization-sensitive computational imaging technique—polarization-sensitive intensity diffraction tomography (PS-IDT)—that enables the reconstruction of 3D anisotropy distribution of both weakly and multiple scattering specimens from multiple intensity-only measurements. A 3D anisotropic object is illuminated by circularly polarized plane waves at various illumination angles to encode the isotropic and anisotropic structural information into 2D intensity information. These information are then recorded separately through two orthogonal analyzer states, and a 3D Jones matrix is iteratively reconstructed based on the vectorial multi-slice beam propagation model and gradient descent method. We demonstrate the 3D anisotropy imaging capabilities of PS-IDT by presenting 3D anisotropy maps of various samples, including potato starch granules and tardigrade.
Role of the gut microbiota in anticancer therapy: from molecular mechanisms to clinical applications
Authors: Zhao Lin-Yong , Mei Jia-Xin , Yu Gang , Lei Lei , Zhang Wei-Han , Liu Kai , Chen Xiao-Long , Kołat Damian , Yang Kun , Hu Jian-Kun 
Published: May 2023

AbstractIn the past period, due to the rapid development of next-generation sequencing technology, accumulating evidence has clarified the complex role of the human microbiota in the development of cancer and the therapeutic response. More importantly, available evidence seems to indicate that modulating the composition of the gut microbiota to improve the efficacy of anti-cancer drugs may be feasible. However, intricate complexities exist, and a deep and comprehensive understanding of how the human microbiota interacts with cancer is critical to realize its full potential in cancer treatment. The purpose of this review is to summarize the initial clues on molecular mechanisms regarding the mutual effects between the gut microbiota and cancer development, and to highlight the relationship between gut microbes and the efficacy of immunotherapy, chemotherapy, radiation therapy and cancer surgery, which may provide insights into the formulation of individualized therapeutic strategies for cancer management. In addition, the current and emerging microbial interventions for cancer therapy as well as their clinical applications are summarized. Although many challenges remain for now, the great importance and full potential of the gut microbiota cannot be overstated for the development of individualized anti-cancer strategies, and it is necessary to explore a holistic approach that incorporates microbial modulation therapy in cancer.
Immunosenescence: molecular mechanisms and diseases
Authors: Liu Zaoqu , Liang Qimeng , Ren Yuqing , Guo Chunguang , Ge Xiaoyong , Wang Libo , Cheng Quan , Luo Peng , Zhang Yi , Han Xinwei 
Published: May 2023

AbstractInfection susceptibility, poor vaccination efficacy, age-related disease onset, and neoplasms are linked to innate and adaptive immune dysfunction that accompanies aging (known as immunosenescence). During aging, organisms tend to develop a characteristic inflammatory state that expresses high levels of pro-inflammatory markers, termed inflammaging. This chronic inflammation is a typical phenomenon linked to immunosenescence and it is considered the major risk factor for age-related diseases. Thymic involution, naïve/memory cell ratio imbalance, dysregulated metabolism, and epigenetic alterations are striking features of immunosenescence. Disturbed T-cell pools and chronic antigen stimulation mediate premature senescence of immune cells, and senescent immune cells develop a proinflammatory senescence-associated secretory phenotype that exacerbates inflammaging. Although the underlying molecular mechanisms remain to be addressed, it is well documented that senescent T cells and inflammaging might be major driving forces in immunosenescence. Potential counteractive measures will be discussed, including intervention of cellular senescence and metabolic-epigenetic axes to mitigate immunosenescence. In recent years, immunosenescence has attracted increasing attention for its role in tumor development. As a result of the limited participation of elderly patients, the impact of immunosenescence on cancer immunotherapy is unclear. Despite some surprising results from clinical trials and drugs, it is necessary to investigate the role of immunosenescence in cancer and other age-related diseases.
USP28 controls SREBP2 and the mevalonate pathway to drive tumour growth in squamous cancer
Authors: Maier Carina R. , Hartmann Oliver , Prieto-Garcia Cristian , Al-Shami Kamal M. , Schlicker Lisa , Vogel Felix C. E. , Haid Silke , Klann Kevin , Buck Viktoria , Münch Christian , Schmitz Werner , Einig Elias , Krenz Bastian , Calzado Marco A. , Eilers Martin , Popov Nikita , Rosenfeldt Mathias T. , Diefenbacher Markus E. , Schulze Almut 
Published: May 2023

AbstractSREBP2 is a master regulator of the mevalonate pathway (MVP), a biosynthetic process that drives the synthesis of dolichol, heme A, ubiquinone and cholesterol and also provides substrates for protein prenylation. Here, we identify SREBP2 as a novel substrate for USP28, a deubiquitinating enzyme that is frequently upregulated in squamous cancers. Our results show that silencing of USP28 reduces expression of MVP enzymes and lowers metabolic flux into this pathway. We also show that USP28 binds to mature SREBP2, leading to its deubiquitination and stabilisation. USP28 depletion rendered cancer cells highly sensitive to MVP inhibition by statins, which was rescued by the addition of geranyl-geranyl pyrophosphate. Analysis of human tissue microarrays revealed elevated expression of USP28, SREBP2 and MVP enzymes in lung squamous cell carcinoma (LSCC) compared to lung adenocarcinoma (LADC). Moreover, CRISPR/Cas-mediated deletion of SREBP2 selectively attenuated tumour growth in a KRas/p53/LKB1 mutant mouse model of lung cancer. Finally, we demonstrate that statins synergise with a dual USP28/25 inhibitor to reduce viability of SCC cells. Our findings suggest that combinatorial targeting of MVP and USP28 could be a therapeutic strategy for the treatment of squamous cell carcinomas.
Benzimidazole carbamate induces cytotoxicity in breast cancer cells via two distinct cell death mechanisms
Authors: Graff Brendan T. , Palanivel Chitra , Jenkins Christopher B. , Baranowska-Kortylewicz Janina , Yan Ying 
Published: May 2023

AbstractMetastatic breast cancer (mBC) is responsible for >90% of breast cancer-related deaths. Microtubule-targeting agents (MTAs) are the front-line treatment for mBC. However, the effectiveness of MTAs is frequently limited by the primary or acquired resistance. Furthermore, recurrent mBC derived from cancer cells that survived MTA treatment are typically more chemoresistant. The overall response rates for the second- and third-line MTAs in mBC patients previously treated with MTAs are 12–35%. Thus, there is an ongoing search for novel MTAs with a distinct mode of action that can circumvent chemoresistance mechanisms. Our results show that methyl N-(6-benzoyl-1H-benzimidazol-2-yl)carbamate (BCar), a microtubule-disrupting anthelmintic that binds to the colchicine binding site separate from the binding sites of clinically used MTAs, has the potential to treat MTA-resistant mBC. We have comprehensively evaluated the cellular effects of BCar in a panel of human breast cancer (BC) cell lines and normal breast cells. BCar effects on the clonogenic survival, cell cycle, apoptosis, autophagy, senescence, and mitotic catastrophe were measured. Approximately 25% of BCs harbor mutant p53. For this reason, the p53 status was included as a variable. The results show that BC cells are >10x more sensitive to BCar than normal mammary epithelial cells (HME). p53-mutant BC cells are significantly more sensitive to BCar treatment than p53 wild-type BC cells. Furthermore, BCar appears to kill BC cells primarily via either p53-dependent apoptosis or p53-independent mitotic catastrophe. When compared to docetaxel and vincristine, two clinical MTAs, BCar is fairly innocuous in HME cells, providing a much wider therapeutic window than docetaxel and vincristine. Together, the results strongly support the notion that BCar-based therapeutics may serve as a new line of MTAs for mBC treatment.
Structure of a eukaryotic cholinephosphotransferase-1 reveals mechanisms of substrate recognition and catalysis
Authors: Wang Lie , Zhou Ming 
Published: May 2023

AbstractPhosphatidylcholine (PC) is the most abundant phospholipid in eukaryotic cell membranes. In eukaryotes, two highly homologous enzymes, cholinephosphotransferase-1 (CHPT1) and choline/ethanolamine phosphotransferase-1 (CEPT1) catalyze the final step of de novo PC synthesis. CHPT1/CEPT1 joins two substrates, cytidine diphosphate-choline (CDP-choline) and diacylglycerol (DAG), to produce PC, and Mg2+ is required for the reaction. However, mechanisms of substrate recognition and catalysis remain unresolved. Here we report structures of a CHPT1 from Xenopus laevis (xlCHPT1) determined by cryo-electron microscopy to an overall resolution of ~3.2 Å. xlCHPT1 forms a homodimer, and each protomer has 10 transmembrane helices (TMs). The first 6 TMs carve out a cone-shaped enclosure in the membrane in which the catalysis occurs. The enclosure opens to the cytosolic side, where a CDP-choline and two Mg2+ are coordinated. The structures identify a catalytic site unique to eukaryotic CHPT1/CEPT1 and suggest an entryway for DAG. The structures also reveal an internal pseudo two-fold symmetry between TM3-6 and TM7-10, and suggest that CHPT1/CEPT1 may have evolved from their distant prokaryotic ancestors through gene duplication.
A local water molecular-heating strategy for near-infrared long-lifetime imaging-guided photothermal therapy of glioblastoma
Authors: Kang Dongkyu , Kim Hyung Shik , Han Soohyun , Lee Yeonju , Kim Young-Pil , Lee Dong Yun , Lee Joonseok 
Published: May 2023

AbstractOwing to the strong absorption of water in the near-infrared (NIR) region near 1.0 μm, this wavelength is considered unsuitable as an imaging and analytical signal in biological environments. However, 1.0 μm NIR can be converted into heat and used as a local water-molecular heating strategy for the photothermal therapy of biological tissues. Herein, we describe a Nd-Yb co-doped nanomaterial (water-heating nanoparticles (NPs)) as strong 1.0 μm emissive NPs to target the absorption band of water. Furthermore, introducing Tm ions into the water-heating NPs improve the NIR lifetime, enabling the development of a NIR imaging-guided water-heating probe (water-heating NIR NPs). In the glioblastoma multiforme male mouse model, tumor-targeted water-heating NIR NPs reduce the tumor volume by 78.9% in the presence of high-resolution intracranial NIR long-lifetime imaging. Hence, water-heating NIR NPs can be used as a promising nanomaterial for imaging and photothermal ablation in deep-tissue-bearing tumor therapy.
Implementation of a primary care asthma management quality improvement programme across 68 general practice sites
Authors: Gilchrist Francis J. , Carroll William D. , Clayton Sadie , Price David , Jarrold Ian , Small Iain , Sutton Emma J. , Lenney Warren 
Published: May 2023

AbstractDespite national and international guidelines, asthma is frequently misdiagnosed, control is poor and unnecessary deaths are far too common. Large scale asthma management programme such as that undertaken in Finland, can improve asthma outcomes. A primary care asthma management quality improvement programme was developed with the support of the British Lung Foundation (now Asthma + Lung UK) and Optimum Patient Care (OPC) Limited. It was delivered and cascaded to all relevant staff at participating practices in three Clinical Commissioning Groups. The programme focussed on improving diagnostic accuracy, management of risk and control, patient self-management and overall asthma control. Patient data were extracted by OPC for the 12 months before (baseline) and after (outcome) the intervention. In the three CCGs, 68 GP practices participated in the programme. Uptake from practices was higher in the CCG that included asthma in its incentivised quality improvement programme. Asthma outcome data were successfully extracted from 64 practices caring for 673,593 patients. Primary outcome (Royal College of Physicians Three Questions [RCP3Q]) data were available in both the baseline and outcome periods for 10,328 patients in whom good asthma control (RCP3Q = 0) increased from 36.0% to 39.2% (p < 0.001) after the intervention. The odds ratio of reporting good asthma control following the intervention was 1.15 (95% CI 1.09–1.22), p < 0.0001. This asthma management programme produced modest but highly statistically significant improvements in asthma outcomes. Key lessons learnt from this small-scale implementation will enable the methodology to be improved to maximise benefit in a larger scale role out.
Efficient radioactive gas detection by scintillating porous metal–organic frameworks
Authors: Orfano Matteo , Perego Jacopo , Cova Francesca , Bezuidenhout Charl X. , Piva Sergio , Dujardin Christophe , Sabot Benoit , Pierre Sylvie , Mai Pavlo , Daniel Christophe , Bracco Silvia , Vedda Anna , Comotti Angiolina , Monguzzi Angelo 
Published: May 2023

AbstractNatural and anthropogenic gas radionuclides such as radon, xenon, hydrogen and krypton isotopes must be monitored to be managed as pathogenic agents, radioactive diagnostic agents or nuclear activity indicators. State-of-the-art detectors based on liquid scintillators suffer from laborious preparation and limited solubility for gases, which affect the accuracy of the measurements. The actual challenge is to find solid scintillating materials simultaneously capable of concentrating radioactive gases and efficiently producing visible light revealed with high sensitivity. The high porosity, combined with the use of scintillating building blocks in metal–organic frameworks (MOFs), offers the possibility to satisfy these requisites. We demonstrate the capability of a hafnium-based MOF incorporating dicarboxy-9,10-diphenylanthracene as a scintillating conjugated ligand to detect gas radionuclides. Metal–organic frameworks show fast scintillation, a fluorescence yield of ∼40%, and accessible porosity suitable for hosting noble gas atoms and ions. Adsorption and detection of 85Kr, 222Rn and 3H radionuclides are explored through a newly developed device that is based on a time coincidence technique. Metal–organic framework crystalline powder demonstrated an improved sensitivity, showing a linear response down to a radioactivity value below 1 kBq m−3 for 85Kr, which outperforms commercial devices. These results support the possible use of scintillating porous MOFs to fabricate sensitive detectors of natural and anthropogenic radionuclides.
Integrated microbiome-metabolome-genome axis data of Laiwu and Lulai pigs
Authors: Lai Xueshuang , Zhang Zhenyang , Zhang Zhe , Liu Shengqiang , Bai Chunyan , Chen Zitao , Qadri Qamar Raza , Fang Yifei , Wang Zhen , Pan Yuchun , Wang Qishan 
Published: May 2023

AbstractExcessive fat deposition can trigger metabolic diseases, and it is crucial to identify factors that can break the link between fat deposition and metabolic diseases. Healthy obese Laiwu pigs (LW) are high in fat content but resistant to metabolic diseases. In this study, we compared the fecal microbiome, fecal and blood metabolome, and genome of LW and Lulai pigs (LU) to identify factors that can block the link between fat deposition and metabolic diseases. Our results show significant differences in Spirochetes and Treponema, which are involved in carbohydrate metabolism, between LW and LU. The fecal and blood metabolome composition was similar, and some anti-metabolic disease components of blood metabolites were different between the two breeds of pigs. The predicted differential RNA is mainly enriched in lipid metabolism and glucose metabolism, which is consistent with the functions of differential microbiota and metabolites. The down-regulated gene RGP1 is strongly negatively correlated with Treponema. Our omics data would provide valuable resources for further scientific research on healthy obesity in both human and porcine.
The effect of cranial techniques on the heart rate variability response to psychological stress test in firefighter cadets
Authors: Wójcik Małgorzata , Siatkowski Idzi 
Published: May 2023

AbstractHeart rate variability (HRV) is a simple tool to monitor cardiovascular stress. The proper function of the cardiovascular system is a problem among firefighters. Physical activity has health benefits correlated with psychological stress. Physically active people should be more resilient to psychological stress, but this has not always been demonstrated. The aim of this study was to determine whether cranial techniques would have an effect on HRV parameters. Osteopathy in the cranium reduces stress and improves cardiovascular function. Fifty-seven firefighter cadets aged 18–24 years (21.63 ± 1.41) participated in the study. All subjects had their heart rate variability measured and were randomly assigned either to the cranial techniques (CS) group, with therapy performed once a week for 5 weeks), or to the control group (CO). After 5 weeks, heart rate variability was measured again in both groups. In the Friedman test, in the CS group there was a statistically significant effect of cranial techniques on Heart Rate (HR) and Low Frequency (LF), but not on High Frequency (HF); in the CO group, a statistically significant difference was observed for HR, HF and LF. In the Nemenyi test, in the CS group there was a statistically significant difference for HR and LF and in the CO group for HR, HF and LF. After applying hierarchical clustering with Euclidean measure and the complete method, dendrograms were drawn up showing similarities for HR, HF and LF values. The cranial techniques and touch might exert a beneficial effect on HRV. Both factors can be used in stressful situations to lower HRV.