bluuebunny/crossref_metadata_2025_split huggingface dataset:
Clinical and functional correlates of parkinsonism in a population-based sample of individuals aged 75 + : the Pietà study
Authors: Vale Thiago Cardoso , Cardoso Francisco Eduardo Costa , da Silva Danilo Jorge , Resende Elisa de Paula Franca , Maia Débora Palma , Cunningham Mauro César Quintão , Guimarães Henrique Cerqueira , Machado João Carlos Barbosa , Teixeira Antônio Lúcio , Caramelli Paulo , Barbosa Maira Tonidandel 
Published: July 2023

Abstract
Background
Parkinsonism is strongly associated with ageing, and many studies have suggested that parkinsonian signs may affect up to half of older adults and is associated with a wide range of adverse health outcomes. We compared clinical and functional characteristics of oldest-old community-dwelling individuals with parkinsonism (parkinsonian group [PG]) to individuals without parkinsonism (non-parkinsonian group [NPG].

Methods
The Pietà study is a population-based study conducted in Caeté, southeast Brazil, involving 607 individuals aged 75 + years submitted to an extensive clinical evaluation. A subset of 65 PG individuals (61.5% women, median age of 82 years) was compared to 542 NPG individuals (64.8% women, median age of 80 years).

Results
PG individuals had significantly more functional impairment, clinical comorbidities (including number of falls, loss of bladder control and dysphagia) and major depression. Multivariate analysis revealed that older age, higher UPDRSm scores, lower category fluency test (animals/minute) and delayed recall memory scores were associated with PG. This group was also more cognitively impaired, with lower performance than NPG individuals in the Mini-Mental State Examination, category fluency test (animals/minute), clock drawing and in delayed recall (p < 0.001 for all tests). UPDRSm scores were the most contributing factor to cognition that independently explained variability in functionality of the entire sample.

Conclusion
Individuals aged 75 + years with parkinsonism were significantly more clinically and functionally impaired in this population-based sample. Cognitive dysfunction explained most of the loss of functionality in these patients. UPDRS-m scores contributed independently to explain variability in functionality in the whole sample.
Six polymorphisms in the lncRNA H19 gene and the risk of cancer: a systematic review and meta-analysis
Authors: Yang Maoquan , Zhang Mingwei , Wang Qiong , Guo Xiaojing , Geng Peizhen , Gu Jinhua , Ji Wansheng , Zhang Li 
Published: July 2023

Abstract
Background
Numerous studies have demonstrated long noncoding RNA (lncRNA) play an important role in the occurrence and progression of cancer, and single nucleotide polymorphisms (SNPs) located in lncRNA are considered to affect cancer suspensibility. Herein, a meta-analysis was carried out to better assess the relationship of H19 polymorphisms and cancer susceptibility.

Methods
A literature search was conducted through using PubMed, EMBASE, and Web of Science databases to obtain relevant publications before Aug 23, 2022. The reference lists of the retrieved studies were also investigated to identify additional relevant articles. The pooled odds ratios (ORs) with 95% confidence intervals (CIs) were calculated to appraise the risk of various cancers.

Results
There appeared to be a remarkable correlation between the rs2107425 variation and decreased cancer risk among Caucasians. Nevertheless, the rs217727 polymorphism was significantly associated with an increased risk of lung cancer, hepatocellular carcinoma and oral squamous cell carcinoma. Also, we found a significant correlation between the rs2839698 polymorphism and increased cancer risk among Asians, gastric cancer, hepatocellular carcinoma, hospital-based control and larger simple size subgroups, respectively. Similarly, the rs3741219 mutation was notably related to cancer risk in higher quality score. As for rs3024270 polymorphism, the homozygous model was markedly linked to cancer risk in overall analysis and population-based controls. There was no significant association between the rs3741216 polymorphism and cancer risk.

Conclusion
H19 rs2839698 and rs3024270 were closely associated with overall cancer risk. H19 rs2107425 was related to lower cancer risk among Caucasians, while the rs2839698 was related to increased cancer risk among Asians. Our results supported that H19 SNPs were significantly correlated with cancer risk.
Promising approaches for the assembly of the catalytically active, recombinant Desulfomicrobium baculatum hydrogenase with substitutions at the active site
Authors: Witkowska Malgorzata , Jedrzejczak Robert P. , Joachimiak Andrzej , Cavdar Onur , Malankowska Anna , Skowron Piotr M. , Zylicz-Stachula Agnieszka 
Published: July 2023

Abstract
Background
Hydrogenases (H2ases) are metalloenzymes capable of the reversible conversion of protons and electrons to molecular hydrogen. Exploiting the unique enzymatic activity of H2ases can lead to advancements in the process of biohydrogen evolution and green energy production.

Results
Here we created of a functional, optimized operon for rapid and robust production of recombinant [NiFe] Desulfomicrobium baculatum hydrogenase (Dmb H2ase). The conversion of the [NiFeSe] Dmb H2ase to [NiFe] type was performed on genetic level by site-directed mutagenesis. The native dmb operon includes two structural H2ase genes, coding for large and small subunits, and an additional gene, encoding a specific maturase (protease) that is essential for the proper maturation of the enzyme. Dmb, like all H2ases, needs intricate bio-production machinery to incorporate its crucial inorganic ligands and cofactors. Strictly anaerobic, sulfate reducer D. baculatum bacteria are distinct, in terms of their biology, from E. coli. Thus, we introduced a series of alterations within the native dmb genes. As a result, more than 100 elements, further compiled into 32 operon variants, were constructed. The initial requirement for a specific maturase was omitted by the artificial truncation of the large Dmb subunit. The assembly of the produced H2ase subunit variants was investigated both, in vitro and in vivo. This approach resulted in 4 recombinant [NiFe] Dmb enzyme variants, capable of H2 evolution. The aim of this study was to overcome the gene expression, protein biosynthesis, maturation and ligand loading bottlenecks for the easy, fast, and cost-effective delivery of recombinant [NiFe] H2ase, using a commonly available E. coli strains.

Conclusion
The optimized genetic constructs together with the developed growth and purification procedures appear to be a promising platform for further studies toward fully-active and O2 tolerant, recombinant [NiFeSe] Dmb H2ase, resembling the native Dmb enzyme. It could likely be achieved by selective cysteine to selenocysteine substitution within the active site of the [NiFe] Dmb variant.
Respected but stigmatized: Healthcare workers caring for COVID-19 patients
Authors: Spruijt Ineke , Cronin Anne , Udeorji Frances , Nazir Mamoona , Shehu Samaila , Poix Sebastien , Villanueva Andre , Jansen Niesje , Huitema Ineke , Suurmond Jeanine , Fiekert Kathy 
Published: July 2023

Background
Healthcare workers (HCWs) caring for Corona Virus Disease 2019 (COVID-19) patients are at increased risk of being stigmatized, which compromises their individual mental well-being and the quality of care they deliver. Stigma-reduction interventions may (partly) prevent this. However, there is a lack of in-depth understanding of the experiences and underlying causes of COVID-19 stigma among HCWs, which is needed to design such interventions. We conducted in-depth semi-structured interviews to assess COVID-19 stigma among COVID-19 HCWs in Ireland, Nigeria, The Netherlands, Pakistan, and The Philippines.

Methods
We used a purposive and snowball sampling to recruit a total of 53 HCWs for online interviews (13 in Ireland; 15 in Nigeria; 6 in The Netherlands; 6 in Pakistan; and 13 in The Philippines (2021). After verbatim transcribing interviews, we used a thematic approach for data analysis.

Results
In all countries, stigmatization of COVID-19 HCWs is driven by fear of infection and the perception of HCWs being carriers of the disease amplified by them wearing of scrubs and personal protective equipment. There were differences between countries in the way stigma manifested in self- anticipated and experienced stigma like scolding, discrimination, avoidance, (self-) isolation, and exclusion in public, in the community, at work, and in the household. The stigma resulted in feelings of depression, loneliness, isolation, and the desire to quit one’s job.

Discussion
COVID-19 HCWs from all countries experienced all forms of stigmatization related to their work as a COVID-19 frontliner. This affected their mental well-being, which in turn affects job performance and quality of care, there is a high need to develop stigma reduction tools for HCWs.
STUCTURE STRENGTH ANALYSIS CONVENTIONAL PILE FIXED JACKET PLATFORM IN NATUNA SEA USING FINITE ELEMENT METHOD
Authors: Aditya Berlian Arswendo , Elyanto Redi Yuniansyah 
Published: July 2023

Offshore Platform is a structure or construction which build in offshore territory to support the exploration process or the exploitation of mine. The main function of the offshore platform is to support the top construction including the operational facilities on the water during the operational time safely, in operational condition (normal) even in stormy condition. Modelling of the structure fixed jacket platform, the environment load and the analysis system using Finite Element Methode software. The environmental load (wind,wave,steam) according to 8 eyes wind (0⁰, 45⁰, 90⁰, 135⁰, 180⁰, 225⁰, 270⁰, and 315⁰). From the software analysis we can conclude that the biggest value of the Unity Check in stormy condition is 0.464 state in 766 member and the biggest value in Unity Check in operational condition is 0.396 state in 766 member. It is a good point because it suited with API RP2A WSD 2007, UC value (Unity Check) ≤ 1.00. So in operational condition even in stormy, fixed jacket platform still be able to withstand the material which is accepted. Another result from the minimum of Safety Factor in operational condition in 766 member has SF 2.00, beside in stormy condition which state in 766 member has 1.66 SF value. This result has a good point because it suited with API RP2A WSD 2007 which is in operational condition the minimum of SF value is 2.00 and in stormy condition the minimum of SF value is 1.5.
Gelatin-PVA-AgNPs Triad Composite as Wound Healing Hydrogel with Wounded Skin Surface Protective Efficiency
Authors: Bag Subhendu Sekhar , Bora Anupama , Golder Animes Kr. , Raina Khyati , Haridhasapavalan Krishna Kumar , Thummer Rajkumar P. 
Published: July 2023

Abstract
The development of non-toxic, biocompatible and antibacterial hydrogels are ideal biomaterials as wound dressing material for promoting wound healing. Thus, we report the development of a low-cost biomacromolecule-based triad nanocomposite hydrogel. Gelatin, a collagen hydrolysate based biomacromolecule, polyvinyl alcohol (PVA), and green-synthesized silver nanoparticles (AgNPs) form the hydrogels for wound dressing application. Gelatin enhances the efficiency of the hydrogel blended with synthetic macromolecule PVA. Silver nanoparticles (AgNPs) synthesized using Musa balbisiana (Bhimkol) peels aqueous extract were incorporated into the PVA-Gelatin hydrogel blend. The developed PVA-gelatin-AgNPs triad nanocomposite hydrogel films were characterized and studied by various spectroscopic and analytical techniques. The incorporation of AgNPs into the gelatin-PVA blend offers better stability, improved water vapour transmission rate (WVTR), surface contact angle, swelling ratio and mechanical properties compared to the native PVA-gelatin blend. The PVA-gelatin-AgNPs triad bionanocomposite hydrogel films showed potent antibacterial efficiency against the commonly present microbes in wounds i.e., E.coli and S. aureus. The hydrogel films showed no toxicity against the BJ normal human foreskin fibroblasts cells. Our composite hydrogel was found to accelerate wound healing, efficiently protect the wounded skin surface against exudate accumulation/dehydration, and prevent bacterial growth and infection. Thus, these gelatin-PVA-AgNPs triad nanocomposite hydrogels acts as ideal for wound dressing applications.
Phytochemicals Characterization of Lianqiao (Forsythia suspensa) extract and evaluation of its antivirus and antioxidant activities
Authors: Gao Boyan , Zhu Hanshu , Liu Zhihao , Wang Thomas T.Y. , He Xiaohua , Sun Jianghao , Li Yanfang , Wu Xianli , Pehrsson Pamela , Zhang Yaqiong , Yao Yuanhang , Yu Liangli (Lucy) 
Published: July 2023

Abstract
Forsythia suspensa (Lianqiao) has long been touted for its anti-viral effects in folklore. Although promising, limited research were conducted to evaluate its potential in defending the severe acute respiratory syndrome coronavirus (SARS-CoV-2). This study evaluated the fruits of Forsythia suspensa (Lianqiao), an important economic crop, for the chemical components of its water and ethanol extracts, along with their inhibitory effects on SARS-CoV-2 spike protein binding to angiotensin-converting enzyme 2 (ACE2), inhibitions of ACE2 activity, and capacities to scavenge the selected free radicals. A total of 42 compounds were tentatively identified in the extracts by HPLC-MS/MS analysis. Both water and ethanol extracts of Lianqiao showed great inhibition of ACE2 activity and the binding affinity between SARS CoV-2 spike protein and ACE2. Furthermore, the water extract had greater scavenging capacities against HO●, DPPH● and ABTS●+ than the ethanol extract. Findings of this research contribute to the knowledge of different extraction solvent systems and support the potential of Lianqiao in reducing the risk of coronavirus infection and alleviating the symptoms after infection.
The causal relationship between gastroesophageal reflux disease and chronic kidney disease：A Two-Sample Mendelian Randomization Study
Authors: Liu Shihan , Zhang Lingli , Luo Wenlong 
Published: July 2023

Abstract
Background
The causal relationship between gastroesophageal reflux disease (GRED) and chronic kidney disease (CKD) is unclear. In this paper, Mendelian randomization method was used to determine the causal effect of GRED disease and CKD, and to identify the risk factors of chronic kidney disease.
Method
Two-sample Mendelian randomization (MR) was used to explore the causal relationship between GRED and CKD. We used a dataset from a recent large-scale genome-wide association study (GWAS). Although we use inverse variance weighting (IVW), MR-Egger and weighted median methods for analysis.
Result
There were significant differences in IVW results, suggesting that GRED patients may be causally associated with CKD disease, and GRED patients may have an increased risk of CKD.
Conclusion
GRED may be one of the risk factors for CKD.
Prevalence of internet addiction among medical students and its impact on psychological distress: A cross-sectional study
Authors: Mohamed Khalid Osman , Soumit Sozan Mudather , Elseed Aziza Abdalmahnoud , Allam Wamda Ahmed , Soomit Amro Mudather , Humeda Humeda Suekit 
Published: July 2023

Abstract
Background:
Internet addiction (IA) has been studied extensively worldwide and has various characteristics that could be detrimental to social, psychological, and functional aspects. Additionally, IA has become a global health issue, especially among teenagers and young adults, and different countries have different rates of internet overuse prevalence. Unfortunately, few studies have been done on medical students in Sudan to explore the effect of internet addiction on psychological distress.
Objective:
This study aimed to measure the prevalence of internet addiction, the risk factors associated with it, and its impact on psychological distress among undergraduate medical students.
Methods:
This cross-sectional study was conducted among medical students of all academic levels at National University using a systematic sampling technique. An online structured questionnaire was used, which included socioeconomic data and the 20-item Internet Addiction Test (IAT) to assess the presence and severity of IA, as well as the 10-item Kessler questionnaire to assess psychological distress. Additionally, to assess the association between internet addiction and different variables, a chi-square test was utilized. A p-value of less than 0.05 was considered statistically significant.
Result:
A total number of 307 medical students participated in this study. Most of them were female (63.8%). The majority (78.5%) were aged 18–22 years, and the prevalence of internet addiction was 75.6% (n = 232). The level of addiction was as follows: 39.7% were mild addicts, 33.2% were moderate addicts, and 2.6% were severe addicts. In the chi-square test, the prevalence of internet addiction was significantly higher among students aged 18–22 years (78.4%) and above (70%) than among students aged less than 18 years (57.7%) (P = 0.044). A strong statistically significant association was found between internet addiction and psychological distress (P = 0.00).
Conclusions:
The current study reveals that the prevalence rates of internet addiction among medical students at National University are very high, with addiction affecting psychological health. Therefore, effective intervention strategies are required to enlighten students on how to restrict excessive internet usage and minimize its impact on psychological status.
Comparison of the efficacy of the PHILOS plate and the hook plate in the treatment of isolated humeral greater tuberosity split fractures
Authors: Li Lin , Tao Fulin , Wang Dawei , Dong Jinlei , Zhou Dongsheng , Song Wenhao 
Published: July 2023

Abstract
Introduction
This study aimed at comparing the efficacy of the PHILOS plate and the hook plate for the treatment of the isolate split greater tuberosity fracture of humerus.
Methods
All patients with isolate split humeral greater tuberosity fractures that were treated using PHILOS plate or hook plate from January 2018 to June 2021 were retrospectively investigated. Data on the above-mentioned patient demographics, injury characteristics, preoperative and postoperative imaging, length of incision, operative time, intraoperative blood loss, and outcomes were recorded and then investigated. Furthermore, the inclusion criteria and exclusion criteria were formulated.
Results
A total of 193 patients were diagnosed with proximal humerus fractures in our institution,and 31 patients conformed to the inclusion criteria. In accordance with the different fixed methods, the patients fell into two groups. To be specific, 19 patients in group A were administrated with PHILOS (proximal humeral internal locking system) plate, and 12 patients in group B were administrated with hook plate. No significant difference was identified in sex, age, injury mechanism, dominant side limb (yes or no), shoulder joint dislocation (yes or no) between two groups. However, in Group B, the operative time was shorter, blood loss was less, length of incision was shorter than Group A's Moreover, pain was evaluated at third day and fifth day after surgery, the pain in fifth day was less in group B than group A, pain in third day and last follow up was no difference between two groups. The constant score, the DASH score, the ROM were evaluated at the last follow up, no significant difference was identified between two groups. The complications were also collected, five patients administrated with PHILOS plate were diagnosed subacromial impact, and two patients administrated with hook plate were diagnosed rotator cuff injury.
Conclusion
The hook plate can achieve favorable results in the treatment of isolate split humeral greater tubercle fractures, with less blood loss, shorter operative time and shorter surgical incisions than the PHILOS plate.
Revealing the Roles of Pyroptosis Genes in Immune Infiltration for Intervertebral Disc Degeneration
Authors: Ma Miao , He Xuegang , Luo Zhangbin , Zhang Guangzhi , Wang Yajun , Kang Xuewen 
Published: July 2023

Abstract
Background
The reduction of the nucleus pulposus is a significant contributing factor to intervertebral disc degeneration (IDD), with pyroptosis being a prominent cause. However, further investigation is required to better understand the mechanisms of pyroptosis in IDD. This study aimed to identify the hub genes involved associated with pyroptosis in IDD.
Methods
The expression matrices of two IDD datasets were obtained from the Gene Expression Omnibus dataset. Subsequently, differentially expressed gene (DEG) analyses were performed on both datasets to identify pyroptosis-related DEGs (PRDEGs). PRDEGs underwent correlation analysis, chromosomal location and functional similarity analysis, and a series of bioinformatics methods to identify hub genes. Finally, networks comprising long non-coding ribonucleic acid (lncRNA), microRNA (miRNA), and hub genes were constructed using the Encyclopaedia of RNA Interactomes and LncBase databases.
Results
Following the intersection of DEGs and pyroptosis-related genes, 18 PRDEGs were identified. Gene Ontology and Kyoto Encyclopaedia of Genes and Genomes enrichment analyses revealed that PRDEGs were primarily associated with the response to lipopolysaccharide and nucleotide-binding and oligomerisation domain (NOD)-like receptor (NLR) signalling pathways. Seven PRDEGs, namely NOD2, NLR family caspase activation and recruitment domain (CARD) containing 4, caspase 5, absent in melanoma 2, NLR family pyrin domain containing 3, apoptosis-associated speck-like protein containing a CARD, and tumour necrosis factor, were identified as hub genes. The association of IDD with resting memory CD4+ T cells was confirmed through CIBERSORT. Finally, a competing endogenous RNA network involving the seven PRDEGs was constructed.
Conclusion
Pyroptosis in IDD was associated with two pathways and seven hub genes. Additionally, four lncRNAs played a crucial role in the pathogenesis and progression of IDD. These potential pyroptosis-related biomarkers might offer novel approaches for further exploration into the underlying mechanisms of IDD.
Broad learning system based on adaptive lasso
Authors: Lu Xinyu , Zhang Qian , Liang Tao 
Published: July 2023

Abstract
Broad learning system is an emerging method, which has achieved outstanding performance in regression and classification problems. This paper proposes a novel algorithm called Broad Learning System based on adaptive lasso (AdL1-BLS) to reduce model size and improve model generalization performance. The proposed AdL1-BLS introduces the weighted L1-norm penalty into BLS, adaptive lasso is used to assign different adaptive coefficients to each output weight of BLS. Because of the effect of adaptive coefficients, the output weight of BLS gets varying degrees of compression rates, which avoids the problem of over-sparse network structure and reduces the prediction error of the network, which better solve the BLS over-fitting problem, and improves the generalization of BLS under the premise of ensuring the sparseness of the network. Finally, the experiments on several commonly used regression data sets are carried out to verify the feasibility of the proposed AdL1-BLS. The experiment results indicate that AdL1-BLS can reduce model complexity without loss of prediction accuracy and improve the model interpretability.