bluuebunny/crossref_metadata_2025_split huggingface dataset:
Alpha synuclein modulates mitochondrial Ca2+ uptake from ER during cell stimulation and under stress conditions
Authors: Ramezani Meraj , Wagenknecht-Wiesner Alice , Wang Tong , Holowka David A. , Eliezer David , Baird Barbara A. 
Published: September 2023

AbstractAlpha synuclein (a-syn) is an intrinsically disordered protein prevalent in neurons, and aggregated forms are associated with synucleinopathies including Parkinson’s disease (PD). Despite the biomedical importance and extensive studies, the physiological role of a-syn and its participation in etiology of PD remain uncertain. We showed previously in model RBL cells that a-syn colocalizes with mitochondrial membranes, depending on formation of N-terminal helices and increasing with mitochondrial stress1. We have now characterized this colocalization and functional correlates in RBL, HEK293, and N2a cells. We find that expression of a-syn enhances stimulated mitochondrial uptake of Ca2+ from the ER, depending on formation of its N-terminal helices but not on its disordered C-terminal tail. Our results are consistent with a-syn acting as a tether between mitochondria and ER, and we show increased contacts between these two organelles using structured illumination microscopy. We tested mitochondrial stress caused by toxins related to PD, 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP/MPP+) and carbonyl cyanide m-chlorophenyl hydrazone (CCCP) and found that a-syn prevents recovery of stimulated mitochondrial Ca2+ uptake. The C-terminal tail, and not N-terminal helices, is involved in this inhibitory activity, which is abrogated when phosphorylation site serine-129 is mutated (S129A). Correspondingly, we find that MPTP/MPP+ and CCCP stress is accompanied by both phosphorylation (pS129) and aggregation of a-syn. Overall, our results indicate that a-syn can participate as a tethering protein to modulate Ca2+ flux between ER and mitochondria, with potential physiological significance. A-syn can also prevent cellular recovery from toxin-induced mitochondrial dysfunction, which may represent a pathological role of a-syn in the etiology of PD.
Realization of quantum signal processing on a noisy quantum computer
Authors: Kikuchi Yuta , Mc Keever Conor , Coopmans Luuk , Lubasch Michael , Benedetti Marcello 
Published: September 2023

AbstractQuantum signal processing (QSP) is a powerful toolbox for the design of quantum algorithms and can lead to asymptotically optimal computational costs. Its realization on noisy quantum computers without fault tolerance, however, is challenging because it requires a deep quantum circuit in general. We propose a strategy to run an entire QSP protocol on noisy quantum hardware by carefully reducing overhead costs at each step. To illustrate the approach, we consider the application of Hamiltonian simulation for which QSP implements a polynomial approximation of the time evolution operator. We test the protocol by running the algorithm on the Quantinuum H1-1 trapped-ion quantum computer powered by Honeywell. In particular, we compute the time dependence of bipartite entanglement entropies for Ising spin chains and find good agreements with exact numerical simulations. To make the best use of the device, we determine optimal experimental parameters by using a simplified error model for the hardware and numerically studying the trade-off between Hamiltonian simulation time, polynomial degree, and total accuracy. Our results are the first step in the experimental realization of QSP-based quantum algorithms.
Complete field-induced spectral response of the spin-1/2 triangular-lattice antiferromagnet CsYbSe2
Authors: Xie Tao , Eberharter A. A. , Xing Jie , Nishimoto S. , Brando M. , Khanenko P. , Sichelschmidt J. , Turrini A. A. , Mazzone D. G. , Naumov P. G. , Sanjeewa L. D. , Harrison N. , Sefat Athena S. , Normand B. , Läuchli A. M. , Podlesnyak A. , Nikitin S. E. 
Published: September 2023

AbstractFifty years after Anderson’s resonating valence-bond proposal, the spin-1/2 triangular-lattice Heisenberg antiferromagnet (TLHAF) remains the ultimate platform to explore highly entangled quantum spin states in proximity to magnetic order. Yb-based delafossites are ideal candidate TLHAF materials, which allow experimental access to the full range of applied in-plane magnetic fields. We perform a systematic neutron scattering study of CsYbSe2, first proving the Heisenberg character of the interactions and quantifying the second-neighbor coupling. We then measure the complex evolution of the excitation spectrum, finding extensive continuum features near the 120°-ordered state, throughout the 1/3-magnetization plateau and beyond this up to saturation. We perform cylinder matrix-product-state (MPS) calculations to obtain an unbiased numerical benchmark for the TLHAF and spectacular agreement with the experimental spectra. The measured and calculated longitudinal spectral functions reflect the role of multi-magnon bound and scattering states. These results provide valuable insight into unconventional field-induced spin excitations in frustrated quantum materials.
Integrated Transcriptome Analysis of Long Noncoding RNA and mRNA in Developing and Aging Mouse Retina
Authors: Kong Kangjie , Wang Peiyuan , Xie Zihong , Wang Lu , Jiang Jiaxuan , Liu Yaoming , Du Shaolin , Jiang Jingwen , Song Yunhe , Lin Fengbin , Wang Wei , Fang Xiuli , Shi Zhuoxing , Zhang Xiulan , Chen Shida 
Published: September 2023

AbstractMice have emerged as a widely employed model for investigating various retinal diseases. However, the availability of comprehensive datasets capturing the entire developmental and aging stages of the mouse retina, particularly during the elderly period, encompassing integrated lncRNA and mRNA expression profiles, is limited. In this study, we assembled a total of 18 retina samples from mice across 6 distinct stages of development and aging (5 days, 3 weeks, 6 weeks, 10 weeks, 6 months, and 15 months) to conduct integrated lncRNA and mRNA sequencing analysis. This invaluable dataset offers a comprehensive transcriptomic resource of mRNA and lncRNA expression profiles during the natural progression of retinal development and aging. The discoveries stemming from this investigation will significantly contribute to the elucidation of the underlying molecular mechanisms associated with various retinal diseases, such as congenital retinal dysplasia and retinal degenerative diseases.
Synchronization of passes in event and spatiotemporal soccer data
Authors: Biermann Henrik , Komitova Rumena , Raabe Dominik , Müller-Budack Eric , Ewerth Ralph , Memmert Daniel 
Published: September 2023

AbstractThe majority of soccer analysis studies investigates specific scenarios through the implementation of computational techniques, which involve the examination of either spatiotemporal position data (movement of players and the ball on the pitch) or event data (relating to significant situations during a match). Yet, only a few applications perform a joint analysis of both data sources despite the various involved advantages emerging from such an approach. One possible reason for this is a non-systematic error in the event data, causing a temporal misalignment of the two data sources. To address this problem, we propose a solution that combines the SwiftEvent online algorithm (Gensler and Sick in Pattern Anal Appl 21:543–562, 2018) with a subsequent refinement step that corrects pass timestamps by exploiting the statistical properties of passes in the position data. We evaluate our proposed algorithm on ground-truth pass labels of four top-flight soccer matches from the 2014/15 season. Results show that the percentage of passes within half a second to ground truth increases from 14 to 70%, while our algorithm also detects localization errors (noise) in the position data. A comparison with other models shows that our algorithm is superior to baseline models and comparable to a deep learning pass detection method (while requiring significantly less data). Hence, our proposed lightweight framework offers a viable solution that enables groups facing limited access to (recent) data sources to effectively synchronize passes in the event and position data.
An extraocular electrical stimulation approach to slow down the progression of retinal degeneration in an animal model
Authors: Gonzalez Calle Alejandra , Paknahad Javad , Pollalis Dimitrios , Kosta Pragya , Thomas Biju , Tew Ben Yi , Salhia Bodour , Louie Stan , Lazzi Gianluca , Humayun Mark 
Published: September 2023

AbstractRetinal diseases such as retinitis pigmentosa (RP) and age-related macular degeneration (AMD) are characterized by unrelenting neuronal death. However, electrical stimulation has been shown to induce neuroprotective changes in the retina capable of slowing down the progression of retinal blindness. In this work, a multi-scale computational model and modeling platform were used to design electrical stimulation strategies to better target the bipolar cells (BCs), that along with photoreceptors are affected at the early stage of retinal degenerative diseases. Our computational findings revealed that biphasic stimulus pulses of long pulse duration could decrease the activation threshold of BCs, and the differential stimulus threshold between ganglion cells (RGCs) and BCs, offering the potential of targeting the BCs during the early phase of degeneration. In vivo experiments were performed to evaluate the electrode placement and parameters found to target bipolar cells and evaluate the safety and efficacy of the treatment. Results indicate that the proposed transcorneal Electrical Stimulation (TES) strategy can attenuate retinal degeneration in a Royal College of Surgeon (RCS) rodent model, offering the potential to translate this work to clinical practice.
Fluid intake at work in foresters working in different thermal conditions
Authors: Orysiak J. , Młynarczyk M. , Tomaszewski P. 
Published: September 2023

AbstractThe primary aim of this study was to assess the impact of fluid intake on hydration status indices in men at work. The secondary aim was to determine the type of fluids drunk at work in different thermal conditions. Fifty-nine male foresters were examined before and after one working day during summer, autumn, and winter. Before and after work, urine and blood samples were obtained from foresters. Immediately after a shift, participants completed a questionnaire regarding fluid intake during one working day. The amount of fluid consumed affects the hydration urine indices. Urine specific gravity and urine osmolality significantly decreased with increasing fluid intake (r = − 0.385 and r = − 0.405, respectively). Moreover, an impact of season on the type of fluids consumed by workers was observed. Tea was significantly more often chosen by workers to drink in winter (68%) than in summer (32%) (p = 0.026). The consumption of any non-alcoholic fluids contributes to the daily total water intake, but it is necessary to create individualized fluid replacement plans. Workers should know how much and what types of drinks to consume at work.
Key topographic parameters driving surface adhesion of Porphyromonas gingivalis
Authors: Papa Steve , Maalouf Mathieu , Claudel Pierre , Sedao Xxx , Di Maio Yoan , Hamzeh-Cognasse Hind , Thomas Mireille , Guignandon Alain , Dumas Virginie 
Published: September 2023

AbstractDental implant failure is primarily due to peri-implantitis, a consequence of bacterial biofilm formation. Bacterial adhesion is strongly linked to micro-/nano-topographies of a surface; thus an assessment of surface texture parameters is essential to understand bacterial adhesion. In this study, mirror polished titanium samples (Ti6Al4V) were irradiated with a femtosecond laser (fs-L) at a wavelength of 1030 nm (infrared) with variable laser parameters (laser beam polarization, number, spacing and organization of the impacts). Images of 3-D topographies were obtained by focal variation microscopy and analyzed with MountainsMap software to measure surface parameters. From bacteria associated with peri-implantitis, we selectedPorphyromonas gingivalisto evaluate its adhesion on Ti6Al4V surfaces in an in vitro study. Correlations between various surface parameters andP. gingivalisadhesion were investigated. We discovered that Sa value, a common measure of surface roughness, was not sufficient in describing the complexity of these fs-L treated surfaces and their bacterial interaction. We found that Sku, density and mean depths of the furrows, were the most accurate parameters for this purpose. These results provide important information that could help anticipate the bacterial adhesive properties of a surface based on its topographic parameters, thus the development of promising laser designed biofunctional implants.
Awareness, behavior, and determinants of dietary salt intake in adults: results from the National NCD Monitoring Survey, India
Authors: Mathur Prashant , Kulothungan Vaitheeswaran , Nath Anita , Vinay Urs K. S. , Ramakrishnan Lakshmy 
Published: September 2023

AbstractA diet high in sodium contributes to a significant proportion of Disability Adjusted Life Years (DALYs) due to cardiovascular diseases. This paper describes the awareness, behaviour and determinants related to dietary salt intake in an adult population of 18–69 years that were assessed as part of the National NCD Monitoring Survey (NNMS) in India. A sub-sample of 3000 adults selected through simple random sampling from 150 nationally representative Primary Sampling Units (PSUs) was included. Data regarding awareness and behaviour related to dietary salt intake were collected. Urinary sodium excretion in spot urine samples was estimated and used to calculate dietary salt intake. The dietary salt intake’s sociodemographic, behavioural and metabolic determinants were also analysed. Less than one-third of the adults of both genders in all age groups in rural and urban areas were aware that daily high salt intake could affect health. The estimated mean daily salt intake was 8.0 g (8.9 g/day for men and 7.1 g/day for women). The salt intake was significantly higher in men [Adjusted OR = 17.66 (5.24–59.46)], rural areas [Adjusted OR = 6.14 (1.83–20.60)], overweight and obese respondents [Adjusted OR = 17.62 (3.17–98.07)]. The perception of the harmful effects of high salt intake and practices to limit salt intake was low in the study population. The mean daily salt intake was higher than the WHO recommendation of up to 5 g daily. The mean dietary salt intake is high in the Indian population, which calls for planning and implementing control of dietary salt consumption measures.
Green synthesis, characterization, anti-SARS-CoV-2 entry, and replication of lactoferrin-coated zinc nanoparticles with halting lung fibrosis induced in adult male albino rats
Authors: El-Fakharany Esmail M. , El-Maradny Yousra A. , Ashry Mahmoud , Abdel-Wahhab Khaled G. , Shabana Marwa E. , El-Gendi Hamada 
Published: September 2023

AbstractThe ethanolic extract of Coleus forskohlii Briq leaves was employed in the green synthesis of zinc nanoparticles (Zn-NPs) by an immediate, one-step, and cost-effective method in the present study. Zn-NPs were coated with purified bovine lactoferrin (LF) and characterized through different instrumental analysis. The biosynthesized Zn-NPs were white in color revealing oval to spherical-shaped particles with an average size of 77 ± 5.50 nm, whereas LF-coated Zn-NPs (LF-Zn-NPs) revealed a larger particles size of up to 98 ± 6.40 nm. The biosynthesized Zn-NPs and LF-Zn-NPs revealed negatively charged surfaces with zeta-potentials of – 20.25 ± 0.35 and – 44.3 ± 3.25 mV, respectively. Interestingly, the LF-Zn-NPs showed potent in vitro retardation for SARS-CoV-2 entry to host cells by binding to the ACE2-receptor and spike protein receptor binding domain at IC50 values of 59.66 and μg/mL, respectively. Additionally, the results indicated the ability of LF-Zn-NPs to inhibit SARS-CoV-2 replication by interfering with RNA-dependent RNA polymerase “RdRp” activity at IC50 of 49.23 μg/mL. In vivo, the LF-Zn-NPs displayed a protective and therapeutic activity against induced pulmonary fibrosis in Bleomycin-treated male albino rats owing to its anti-inflammatory, antioxidant, and significant reduction in CRP, LDH, ferritin, and D-dimer levels. The obtained findings offer a promising route for biosynthesized Zn-NPs and LF-Zn-NPs as promising candidates against COVID-19.
Ultra-fast adsorption of radioactive technetium (99mTc) by using mining waste clay samples, Abu-Tartur, Egypt
Authors: Ahmed Ahmed Saleh , Hassan Walaa Ali , Mohamed Mohamed Abdel-Moneim , Ahmed Ezzat Abdalla , Shaalan Nagih M. , Abukhadra Mostafa Ragab 
Published: September 2023

AbstractIn this study, we have opened a great route to fabricate a high-performance nanocomposite for various functional applications based on the composite of a natural stone. A clay sample (black shale (B.Sh)) was collected from the Abu-Tartur area in Egypt. The black shale was organically modified with organic materials in our laboratory, which is called organo-black shale (O-B.Sh). The samples were characterized by XRD, FTIR, SEM, and XRF. These techniques confirmed that the samples have multi-oxide phases with approximately SiO2 at 54.1%, Al2O3 at 24.73%, Fe2O3 at 6.02%, K2O at 1.12%, MgO at 1.09%, and Na2O of 0.09%, as calculated by XRF. The two samples were applied to the adsorption processes of the radioactive technetium materials, which have been used for the medical treatment of the cancer institute of Upper Egypt. The adsorption processes were performed at various concentrations of the radioactive material and various amounts of clay samples. The as-collected B.Sh sample showed an adsorption activity of 65%, however, the organically modified materials showed a high adsorption rate toward technetium reaches to 100% in a very short time and without any further process. The present collected materials are very promising to withdraw the radioactive materials from the saline solution to save human and environmental health. We believe these multi-compound composites may open a new approach for creating new fabric composites with high performance for various applications.
Unraveling the controversial effect of Covid-19 on college students’ performance
Authors: Bonacini Luca , Gallo Giovanni , Patriarca Fabrizio 
Published: September 2023

AbstractWe disentangle the channels through which Covid-19 has affected the performance of university students by setting up an econometric strategy to identify separately changes in both teaching and evaluation modes, and the short and long term effects of mobility restrictions. We exploit full and detailed information from the administrative archives of one among the first universities to be shut down since the virus spread from Wuhan. The results help solving the inconsistencies in the literature by providing evidence of a composite picture where negative effects such as those caused by the sudden shift to remote learning and by the exposure to mobility restrictions, overlap to opposite effects due to a change in evaluation methods and home confinement during the exam’s preparation. Such overlap of conflicting effects, weakening the signaling role of tertiary education, would add to the learning loss by further exacerbating future consequences on the “Covid” generation.
Cytotoxic immune cells do not affect TDP-43 and p62 sarcoplasmic aggregation but influence TDP-43 localisation
Authors: McCord Bryony , Day Richard M. 
Published: September 2023

AbstractSporadic inclusion body myositis (sIBM) is an idiopathic inflammatory myopathy with invasion of CD8 T cells in muscle and aggregation of proteins in the sarcoplasm. TDP-43 and p62 are two proteins that aggregate in affected muscle, and have been suggested as specific markers for sIBM over other inflammatory myopathies. TDP-43 is also mislocalised from the nucleus to the sarcoplasm in sIBM. It is not clear if inflammation precedes protein aggregation in sIBM. This study investigated if exposure to cytotoxic inflammatory cells caused TDP-43 and p62 aggregation or TDP-43 mislocalisation in cultured myotubes. TALL-104 coculture was highly cytotoxic to myotubes after 24 h. Secretion of IFNγ and TNFα were higher in cocultures compared to monocultured TALL-104 cells, indicating activation. TALL-104 cells attached to and infiltrated myotubes. There was no effect of TALL-104 coculture on TDP-43 or p62 sarcoplasmic aggregate size or frequency. However, there was decreased localisation of TDP-43 to the nucleus with TALL-104 coculture compared to control. In an in vitro setting, cytotoxic immune cells did not cause TDP-43 or p62 sarcoplasmic aggregation, suggesting cellular cytotoxicity may not trigger aggregation of these proteins. However TALL-104 coculture influenced TDP-43 localisation, suggesting cytotoxic immune cells may contribute to TDP-43 localisation shifts which is observed in sIBM.
Noradrenaline depresses facial stimulation-evoked cerebellar MLI-PC synaptic transmission via α2-AR/PKA signaling cascade in vivo in mice
Authors: Wang Jun-Ya , Weng Wen-Cai , Wang Ting-Qi , Liu Yue , Qiu De-Lai , Wu Mao-Cheng , Chu Chun-Ping 
Published: September 2023

AbstractThe noradrenergic fibers of the locus coeruleus, together with mossy fibers and climbing fibers, comprise the three types of cerebellar afferents that modulate the cerebellar neuronal circuit. We previously demonstrated that noradrenaline (NA) modulated synaptic transmission in the mouse cerebellar cortex via adrenergic receptors (ARs). In the present study, we investigated the effect of NA on facial stimulation-evoked cerebellar molecular layer interneuron (MLI)-Purkinje cell (PC) synaptic transmission in urethane-anesthetized mice using an in vivo cell-attached recording technique and a pharmacological method. MLI-PC synaptic transmission was induced by air-puff stimulation (duration: 60 ms) of the ipsilateral whisker pad, which exhibited positive components (P1 and P2) accompanied by a pause in simple spike activity. Cerebellar molecular layer application of NA (15 µM) decreased the amplitude and area under the curve of P1, and the pause in simple spike activity, but increased the P2/P1 ratio. The NA-induced decrease in P1 amplitude was concentration-dependent, and the half-inhibitory concentration was 10.94 µM. The NA-induced depression of facial stimulation-evoked MLI-PC GABAergic synaptic transmission was completely abolished by blockade of α-ARs or α2-ARs, but not by antagonism of α1-ARs or β-ARs. Bath application of an α2-AR agonist inhibited MLI-PC synaptic transmission and attenuated the effect of NA on the synaptic response. NA-induced depression of MLI-PC synaptic transmission was completely blocked by a mixture of α2A- and 2B-AR antagonists, and was abolished by inhibition of protein kinase A. In addition, electrical stimulation of the molecular layer evoked MLI-PC GABAergic synaptic transmission in the presence of an AMPA receptor antagonist, which was inhibited by NA through α2-ARs. Our results indicate that NA inhibits MLI-PC GABAergic synaptic transmission by reducing GABA release via an α2-AR/PKA signaling pathway.