bluuebunny/crossref_metadata_2025_split huggingface dataset:
Influenza A virus exploits transferrin receptor recycling to enter host cells
Authors: Mazel-Sanchez Beryl , Niu Chengyue , Williams Nathalia , Bachmann Michael , Choltus Hélèna , Silva Filo , Serre-Beinier Véronique , Karenovics Wolfram , Iwaszkiewicz Justyna , Zoete Vincent , Kaiser Laurent , Hartley Oliver , Wehrle-Haller Bernhard , Schmolke Mirco 
Published: May 2023

Influenza A virus (IAV) enters host cells mostly through clathrin-dependent receptor-mediated endocytosis. A single bona fide entry receptor protein supporting this entry mechanism remains elusive. Here we performed proximity ligation of biotin to host cell surface proteins in the vicinity of attached trimeric hemagglutinin-HRP and characterized biotinylated targets using mass spectrometry. This approach identified transferrin receptor 1 (TfR1) as a candidate entry protein. Genetic gain-of-function and loss-of-function experiments, as well as in vitro and in vivo chemical inhibition, confirmed the functional involvement of TfR1 in IAV entry. Recycling deficient mutants of TfR1 do not support entry, indicating that TfR1 recycling is essential for this function. The binding of virions to TfR1 via sialic acids confirmed its role as a directly acting entry factor, but unexpectedly even headless TfR1 promoted IAV particle uptake in
trans
. TIRF microscopy localized the entering virus-like particles in the vicinity of TfR1. Our data identify TfR1 recycling as a revolving door mechanism exploited by IAV to enter host cells.
IRIS: Discovery of cancer immunotherapy targets arising from pre-mRNA alternative splicing
Authors: Pan Yang , Phillips John W. , Zhang Beatrice D. , Noguchi Miyako , Kutschera Eric , McLaughlin Jami , Nesterenko Pavlo A. , Mao Zhiyuan , Bangayan Nathanael J. , Wang Robert , Tran Wendy , Yang Harry T. , Wang Yuanyuan , Xu Yang , Obusan Matthew B. , Cheng Donghui , Lee Alex H. , Kadash-Edmondson Kathryn E. , Champhekar Ameya , Puig-Saus Cristina , Ribas Antoni , Prins Robert M. , Seet Christopher S. , Crooks Gay M. , Witte Owen N. , Xing Yi 
Published: May 2023

Alternative splicing (AS) is prevalent in cancer, generating an extensive but largely unexplored repertoire of novel immunotherapy targets. We describe
I
soform peptides from
R
NA splicing for
I
mmunotherapy target
S
creening (IRIS), a computational platform capable of discovering AS-derived tumor antigens (TAs) for T cell receptor (TCR) and chimeric antigen receptor T cell (CAR-T) therapies. IRIS leverages large-scale tumor and normal transcriptome data and incorporates multiple screening approaches to discover AS-derived TAs with tumor-associated or tumor-specific expression. In a proof-of-concept analysis integrating transcriptomics and immunopeptidomics data, we showed that hundreds of IRIS-predicted TCR targets are presented by human leukocyte antigen (HLA) molecules. We applied IRIS to RNA-seq data of neuroendocrine prostate cancer (NEPC). From 2,939 NEPC-associated AS events, IRIS predicted 1,651 epitopes from 808 events as potential TCR targets for two common HLA types (A*02:01 and A*03:01). A more stringent screening test prioritized 48 epitopes from 20 events with “neoantigen-like” NEPC-specific expression. Predicted epitopes are often encoded by microexons of ≤30 nucleotides. To validate the immunogenicity and T cell recognition of IRIS-predicted TCR epitopes, we performed in vitro T cell priming in combination with single-cell TCR sequencing. Seven TCRs transduced into human peripheral blood mononuclear cells (PBMCs) showed high activity against individual IRIS-predicted epitopes, providing strong evidence of isolated TCRs reactive to AS-derived peptides. One selected TCR showed efficient cytotoxicity against target cells expressing the target peptide. Our study illustrates the contribution of AS to the TA repertoire of cancer cells and demonstrates the utility of IRIS for discovering AS-derived TAs and expanding cancer immunotherapies.
Requests for concrete actions in interaction
Authors: Lindholm Camilla , Paananen Jenny , Stevanovic Melisa , Weiste Elina , Valkeapää Taina 
Published: May 2023

Abstract
In this study, we examine how support workers produce requests for concrete actions and, in this way, manage client participation in mental health rehabilitation. Drawing on Finnish rehabilitation group meetings as data and on conversation analysis, we examine how support workers design their requests for concrete action from clients, how clients respond, and how support workers deal with clients’ responses. The results reveal that support workers tend to use verbs indicating willingness when implementing their requests, whereas clients resort to the modality of possibility. By orienting to willingness, the support workers invoke clients’ sense of responsibility to contribute to group activities and simultaneously avoid questioning their capabilities. On the other hand, clients orient toward the underlying assumptions of social responsibility rather than to their own personal preferences. To conclude, our study demonstrates how support workers address the dilemma of increasing client participation and showing respect for client self-determination.
Investigation on external quantum efficiency droops and inactivation efficiencies of AlGaN-based ultraviolet-c LEDs at 265–285 nm
Authors: Guo Ziquan , Li Zhihui , Lai Shouqiang , Hou Xiaoyang , Fan Xiaotong , Zhong Chenming , Lin Yue , Chen Guolong , Qin Guoheng , Gao Tao , Fu Nuoyi , Shi Yuan , Liao Xinqin , Lin Yi , Lu Yijun , Guo Weijie , Chen Zhong 
Published: May 2023

Abstract
The temperature-dependent external quantum efficiency (EQE) droops of 265 nm, 275 nm, 280 nm, and 285 nm AlGaN-based ultraviolet-c light-emitting diodes (UVC-LEDs) differed in Al contents have been comprehensively investigated. The modified ABC model (R = An + Bn
2 + Cn
3) with the current-leakage related term, f(n) = Dn
4, has been employed to analyze the recombination mechanisms in these UVC-LED samples. Experimental results reveal that, at relatively low electrical-current levels, the contribution of Shockley–Read–Hall (SRH) recombination exceeds those of the Auger recombination and carrier leakage. At relatively high electrical-current levels, the Auger recombination and carrier leakage jointly dominate the EQE droop phenomenon. Moreover, the inactivation efficiencies of 222 nm excimer lamp, 254 nm portable Mercury lamp, 265 nm, 280 nm, and 285 nm UVC-LED arrays in the inactivation of Escherichia coli have been experimentally investigated, which could provide a technical reference for fighting against the new COVID-19.
MiRNA-520a-3p combined with folic acid conjugated Fe2O3@PDA multifunctional nanoagents for MR imagine and antitumor gene-photothermal therapy
Authors: Li Xue , Wang Shuang , Gao Qingzhe , Li Na , Dong Shanshan , Gao Yuwei , Wang Zuobin , Zhang Butian , He Xiuxia 
Published: May 2023

Abstract
Osteosarcoma (OS) is a primary malignant bone tumor that occurs mainly in adolescents. Researchers are devoting to develop combination therapy methods in a multifunctional nanoplatform for the treatment of osteosarcoma. The results of previous research have shown that up-regulation of miR-520a-3p could induce anticancer effects in osteosarcoma. In order to improve the effect of gene therapy (GT), we attempted to carry miR-520a-3p in a multifunctional vector for comprehensive therapy. Fe2O3 is a type of magnetic resonance imaging (MRI) contrast that is widely used as a drug delivery agent. When coated with polydopamine (PDA), it can also be used as a photothermal therapy (PTT) agent (Fe2O3@ PDA). To deliver nanoagents targeted to a tumor site, folic acid (FA) conjugated with Fe2O3 @PDA was manufactured as FA-Fe2O3@PDA. FA was chosen as the target molecule to enhance utilization and reduce toxicity of nanoparticles. However, the therapeutic efficacy of FA-Fe2O3-PDA combined with miR-520a-3p has not yet been studied. In this study, we synthesized FA-Fe2O3@PDA-miRNA and investigated the potential of combining PDA regulated PTT and miR-520a-3p regulated GT to kill osteosarcoma cells. The results indicated that down-regulation of interleukin 6 receptor (IL6R) by miR-520a-3p and the photothermal ability of PDA could induce satisfactory anticancer effects in osteosarcoma, and the curative ratio was better than that used alone PTT or GT. Moreover, as a kind of T
2 magnetic contrast, miRNA-Fe2O3@PDA-FA can be used for MRI. These findings indicated that miRNA-Fe2O3@PDA-FA is an effective anti-tumor nanovector for PTT combined with GT.
On Krylov methods for large-scale CBCT reconstruction
Authors: Sabaté Landman Malena , Biguri Ander , Hatamikia Sepideh , Boardman Richard , Aston John , Schönlieb Carola-Bibiane 
Published: May 2023

Abstract
Krylov subspace methods are a powerful family of iterative solvers for linear systems of equations, which are commonly used for inverse problems due to their intrinsic regularization properties. Moreover, these methods are naturally suited to solve large-scale problems, as they only require matrix-vector products with the system matrix (and its adjoint) to compute approximate solutions, and they display a very fast convergence. Even if this class of methods has been widely researched and studied in the numerical linear algebra community, its use in applied medical physics and applied engineering is still very limited. e.g. in realistic large-scale computed tomography (CT) problems, and more specifically in cone beam CT (CBCT). This work attempts to breach this gap by providing a general framework for the most relevant Krylov subspace methods applied to 3D CT problems, including the most well-known Krylov solvers for non-square systems (CGLS, LSQR, LSMR), possibly in combination with Tikhonov regularization, and methods that incorporate total variation regularization. This is provided within an open source framework: the tomographic iterative GPU-based reconstruction toolbox, with the idea of promoting accessibility and reproducibility of the results for the algorithms presented. Finally, numerical results in synthetic and real-world 3D CT applications (medical CBCT and μ-CT datasets) are provided to showcase and compare the different Krylov subspace methods presented in the paper, as well as their suitability for different kinds of problems.
Evaluation and validation of radial impurity density profiles from CXRS using neutral beam modelling in W7-X
Authors: Romba T , Reimold F , Jaspers R J E , Edmondson A J , Ford O P , Geiger B , Jabłoński S , Kubkowska M , Neelis T W C , Poloskei P Zs , Vanó L , Klinger T 
Published: May 2023

Abstract
Absolute radial impurity density profiles in the Wendelstein 7-X stellarator (W7-X) are derived from charge exchange recombination spectroscopy using modelling of the neutral beam. The approach is validated via cross comparisons of the neutral beam attenuation, the radial localization of ion temperature measurements, as well as a comparison of the effective plasma charge determined by other diagnostics. The latter implies the validity of the obtained absolute density levels. The simulation based approach novel to W7-X allows to assess the neutral beam halo population, introducing corrections to the shape and amplitude of determined impurity density profiles. To illustrate the capabilities of the derived impurity density profiles, the particle transport properties in a W7-X discharge heated by electron cyclotron resonance heating were assessed. Density profiles for various impurities are found to be flat, consistent with dominant anomalous diffusion in the range 0.1–5.0 m2 s−1. This range of the anomalous transport in such plasmas is in line with other impurity diagnostics at W7-X.
Firmware implementation of a recurrent neural network for the computation of the energy deposited in the liquid argon calorimeter of the ATLAS experiment
Authors: Aad G. , Calvet T. , Chiedde N. , Faure R. , Fortin E.M. , Laatu L. , Monnier E. , Sur N. 
Published: May 2023

Abstract
The ATLAS experiment measures the properties of particles
that are products of proton-proton collisions at the LHC. The
ATLAS detector will undergo a major upgrade before the high
luminosity phase of the LHC. The ATLAS liquid argon calorimeter
measures the energy of particles interacting electromagnetically in
the detector. The readout electronics of this calorimeter will be
replaced during the aforementioned ATLAS upgrade. The new electronic
boards will be based on state-of-the-art field-programmable gate
arrays (FPGA) from Intel allowing the implementation of neural
networks embedded in firmware. Neural networks have been shown to
outperform the current optimal filtering algorithms used to compute
the energy deposited in the calorimeter. This article presents the
implementation of a recurrent neural network (RNN) allowing the
reconstruction of the energy deposited in the calorimeter on Stratix
10 FPGAs. The implementation in high level synthesis (HLS) language
allowed fast prototyping but fell short of meeting the stringent
requirements in terms of resource usage and latency. Further
optimisations in Very High-Speed Integrated Circuit Hardware
Description Language (VHDL) allowed fulfilment of the requirements
of processing 384 channels per FPGA with a latency smaller than
125 ns.
CO2 fertilization enhances vegetation productivity and reduces ecological drought in India
Authors: Dubey Nivedita , Ghosh Subimal 
Published: May 2023

Abstract
Higher warming will affect more regions globally with intensified agricultural and ecological droughts. Higher CO2 concentration improves vegetation’s water use efficiency (WUE), but its potential to alleviate extreme agricultural and ecological droughts is unclear. India is the second-highest contributor to global greening, having two of the eight global hottest biodiversity hotspots. Here, for the first time, using the CMIP6 earth system models (ESMs), we found an increase in the net vegetation productivity in India at the rate of 10.552 TgC year−1 with 1% per year increase in atmospheric CO2 concentration from 285 ppm to 1140 ppm, contrary to global trends. The improved WUE resulting from carbon fertilization and higher rain under warming will supersede the increased evapotranspiration water loss due to radiative effects. We found that the substantial increase in vegetation productivity in India attributes to plant physiology, and such factor needs to be considered in the drought projections.
Numerical simulation of a floating-type point absorber wave energy converter
Authors: Srinivasamurthy Sharath 
Published: May 2023

Abstract
Wave energy converters (WEC) have been researched extensively to harvest large amounts of untapped ocean renewable energy. Floating-type point absorbers are a type of WEC that converts the ocean wave energy to electrical energy, by utilizing the relative motion of multi-body system mainly consisting of a floating section and a reaction section. The design of floating-type point absorbers is not straightforward and needs special attention to understand the dynamics of the multi-body system, and power take-off (PTO) mechanism placed in the reaction section. The purpose of this study is to develop a numerical simulation tool which can help understand various dynamics of a floating-type WEC and aid in the practical design of PTO systems. The PTO mechanism is represented using a spring-damper system and theoretical modelling of mean absorbed power is presented. The numerical simulation is developed in Orcaflex and is carried out on a demonstration model to show the usefulness of the developed tool. The heave displacement shows the effect of damping (PTO) on the motion response of the floating-type WEC. It is found that the maximization of absorbed power is possible using the developed simulation tool for a specific sea state. Furthermore, the results show the potential use of the developed tool for other floating-type WEC concepts.
Numerical Analysis of Double Stack Blade Savonius Wind Turbine with Secondary Blades
Authors: Ajarostaghi Seyed Soheil Mousavi , Mousavi Seyed Sina , Bhojaraju Chandrasekhar 
Published: May 2023

Abstract
In this work, the performance of the Savonius vertical axis wind turbine with secondary blades was investigated numerically. The impacts of two geometric parameters of the secondary blades, including the height of secondary blades (h) and the distance between the main and the secondary blades (b) on the output torque were analyzed. Numerical simulations were performed by a commercial CFD code, ANSYS FLUENT 18.2. Numerical results show that employing secondary blades has a constructive impact on the output torque compared to the case without secondary blades. Moreover, the studied geometric parameters of the secondary blades have a significant impoact on the performance of the proposed turbine. It can be seen that by increasing the height of secondary blades, the angular position of the maximum torque is shifted. At b = 2.5 mm, the difference between the models is more comparable. Models h = 6 and 12 mm have the highest output torque, although with a slight difference between them. Model h = 12 mm has the highest output torque. At b = 7.5 mm, the difference between the models is more comparable than b = 2.5 mm. Model h = 12 mm has the highest output torque and models h = 3 and 6 mm are in the next levels. Obtained results in the second section demonstrate that by growing the distance between the secondary and the main blades, in the case of h = 3 mm, the produced torque declines. Furthermore, by increasing the distance between the secondary and the main blades from b = 0 mm to b = 2.5 mm and b = 7.5 mm, the amount of torque produced decreases by 17.33 and 26.66%, respectively. Moreover, by augmenting the distance between the secondary and main blades from b = 2.5 mm to b = 7.5 mm (200% growth), the produced torque decreases by 11.3%.
Rubberized Geopolymer Mortar and Concrete: A Comprehensive Review.
Authors: Arjun Raj P K , Sarath D , Nagarajan Praveen , Shashikala A. P. 
Published: May 2023

Abstract
In many crucial structural applications, a material’s ability to withstand impact is of the utmost importance. The use of crumb rubber particles as a partial replacement of natural aggregates forms rubberized geopolymer concrete (RuGPC), which is found to have great impact resistance and energy absorption capacity. Geopolymer paste and natural aggregates alone are weak in handling the impact loads. The field of Civil engineering has been looking into ways for the last few years to use solid waste and ecologically friendly raw materials as components of concrete in building to embrace sustainability. Greenhouse gas emissions from cement manufacturing and the depletion of natural aggregates are two major issues the construction industry is currently experiencing. RuGPC, which combines the benefits of geopolymer concrete (GPC) and rubberized concrete to create a practical, sustainable building material, has been a hot topic for the past few years. In this paper, the fresh properties, strength and durability characteristics, mixing procedure and curing properties, dynamic and impact properties, microstructures and thermal properties of rubberized geopolymer mortar and concrete are comprehensively reviewed.
Study on the effectiveness of prefabricated cage system reinforcement in columns
Authors: Thejarathnam T , Prashanth M H , Kuttagola Imran 
Published: May 2023

Abstract
The concrete is the primary vertical load bearing component in a reinforced concrete column whereas the steel cage provides additional vertical load carrying capacity along with the confinement of core concrete. The research paper will focus on the performance of Prefabricated Cage System (PCS) Reinforcement in square columns. Eight columns were casted and tested, out of which two are rebar reinforced columns and six are PCS reinforced columns. There were two test groups, each group containing one rebar column and three PCS columns. In this study work is done to compare the performance of a PCS reinforced column to a rebar reinforced column, in addition comparing PCS cages of differently sized grid openings. The objective is to theoretically, experimentally, and numerically investigate the PCS column for its load carrying capacity and displacement capacity. From the results it is observed that as the size of opening of a PCS reinforced column increase, the load carrying capacity increases as the concrete running through the openings can strengthen the connection. Hence to improve the effectiveness of the PCS columns the spacing of openings should be increased. Further, the stiffness and energy absorption are more in rebar reinforced column compared to all PCS columns.
Numerical investigation of the hybrid reinforced concrete beam using GFRP bars
Authors: Kumar Aditya , Prashanth M H , Kuttagola Imran 
Published: May 2023

Abstract
The numerical study of the hybrid reinforced concrete beam using GFRP bars has been explored in this research. Finite element software ABAQUS had been used for numerical investigation. In the present research, the use of GFRP bars as a replacement to steel reinforcing bars for longitudinal reinforcement is attempted. Further, a RC beam by using GFRP as longitudinal reinforcement and steel rebars as transverse reinforcement is compared with the conventional steel reinforced concrete beam. The beams are tested for three-point bending using numerical simulation under displacement control. By performing the numerical modelling, load, strain and displacement data has been attained. Further, by using the output data, stress-strain curves of concrete, load versus strain variations in tension bars and load versus deflection curves are plotted. The stresses, deflections, strain developed in longitudinal bars, load carrying capacity, energy absorption are then calculated using the data and are compared. From the results it is observed that, the load carrying capacity decreases by 16% and the energy absorption increases by 29% when longitudinal steel bars are replaced by GFRP bars.
Supplemented desalinated seawater using sulfuric acid activated zeolit as hydroponic medium for mustard (Brassica juncea L.)
Authors: Iriany A , Hasanah F , Widyanto N D , Farahdina F A R 
Published: May 2023

Abstract
Sufficient agricultural water for irrigation is an important factor in food production, yet available fresh water only occupies 1% of the water on the earth. Seawater, through desalination, becomes rational solution to provide agricultural water due to abundant quantities and beneficial nutrients for crops. Hydroponic using desalinated seawater as medium is an approach to accomplish food security, especially in the arid and coastal areas. This study aimed to examine the growth of mustard (Brassica juncea L.) on a blend of fresh water and desalinated seawater using sulfuric acid activated zeolite as hydroponic medium. The experiment consisted of one control (100% fresh water+AB mix) and treatments namely a blend of 70% fresh water and 30% desalinated seawater using sulfuric acid activated zeolite with various sulfuric acid concentrations i.e 0.5, 1, 1.5, 2, 2.5, 3, and 3.5 N. The growth of mustard in blend of 70% fresh water and 30% desalinated seawater was lower than fresh water. A blend of 70% fresh water and 30% desalinated seawater using 1.5 N sulfuric acid activated zeolite showed no significant different mustard growth compared with fresh water medium and the fresh weight reduction was about 24%. Insignificant different growth between optimum treatment and control indicated a simple desalination method in this study can be utilized to desalinating seawater for hydroponic medium purpose. This finding emphasized that the simple seawater desalination method in this study for hydroponic medium was promising and interesting to be further developed for sustainable water management and agriculture.