bluuebunny/crossref_metadata_2025_split huggingface dataset:
Sweetwater: an underrated crude glycerol for sustainable lipid production in non-conventional yeasts
Authors: Keita Valériane Malika , Lee Yi Qing , Lakshmanan Meiyappan , Ow Dave Siak-Wei , Staniland Paul , Staniland Jessica , Savill Ian , Tee Kang Lan , Wong Tuck Seng , Lee Dong-Yup 
Published: September 2023

AbstractSweetwater, a methanol-free by-product of the fat splitting process, is an emerging alternative feedstock for microbial utilization of crude glycerol. Yeasts are promising candidates in this context due to their versatility in delivering a wide range of value-added products including lipids. To enhance sweetwater utilization, we investigated the growth and lipid production of 21 oleaginous yeast strains in sweetwater and found that nutrient limitation and the unique carbon composition of sweetwater boosted significant lipid accumulation in several strains, in particularRhodosporidium toruloidesNRRL Y-6987. To decipher the underlying mechanism, the transcriptomic changes ofR. toluroidesNRRL Y-6987 were further analyzed, indicating potential sugars and oligopeptides in sweetwater supporting growth and lipid accumulation as well as exogenous fatty acid uptake leading to the enhanced lipid accumulation. Our comparative study successfully demonstrated sweetwater as a cost-effective feedstock and suggested potential sweetwater type and strain engineering targets for increasing microbial lipid production.
Robust of AMBER force field parameters for glutathionylated cysteines
Authors: Elftmaoui Zineb , Bignon Emmanuelle 
Published: September 2023

AbstractS-glutathionylation is an oxidative post-translational modification which is involved in the regulation of many cell signaling pathways. Increasing amounts of studies show that it is crucial in cell homeostasis and deregulated in several pathologies. However, the effect of S-glutathionylation on proteins structure and activity is poorly understood, and a drastic lack of structural information at the atomic scale remains. Studies based on the use of molecular dynamics simulations, which can provide important information about modification-induced modulation of proteins structure and function, are also sparse and there is no benchmarked force field parameters for this modified cysteine. In this contribution, we provide robust AMBER parameters for S-glutathionylation, that we tested extensively against experimental data through a total of 33μs molecular dynamics simulations. We show that our parameters set efficiently describe the global and local structural properties of S-glutathionylated proteins. These data provide the community with an important tool to stimulate investigations about the effect of S-glutathionylation on protein dynamics and function, in a common effort to unravel the structural mechanisms underlying its critical role in cellular processes.
Model selection reveals selective regulation of blood amino acid and lipid metabolism by insulin in humans
Authors: Fujita Suguru , Hironaka Ken-ichi , Karasawa Yasuaki , Kuroda Shinya 
Published: September 2023

SummaryInsulin plays a crucial role in regulating the metabolism of blood glucose, amino acids, and lipids in humans. However, the mechanisms by which insulin selectively controls these different metabolites are not yet fully understood. To address this question, we used mathematical modeling to identify the selective regulatory mechanisms of insulin on blood amino acids and lipids. Our study revealed that insulin negatively regulates the influx and positively regulates the efflux of lipids, which is consistent with previous findings. In contrast, we did not observe the previously reported negative regulation of BCAA influx by insulin; instead, we found that insulin positively regulates BCAA efflux. We also observed that the earlier peak time of lipids compared to BCAAs is dependent on insulin’s negative regulation of their influx. Overall, our findings shed new light on how insulin selectively regulates the levels of different metabolites in human blood, providing valuable insights into the pathogenesis of metabolic disorders and potential therapeutic interventions.
Exploring the Role of YAP1 and TAZ in Pancreatic Acinar Cells and the Therapeutic Potential of VT-104 in Pancreatic Inflammation
Authors: Lopez Kevin , Deng Janice J , Xu Yi , Sharkey Francis E. , Wang Pei , Liu Jun 
Published: September 2023

ABSTRACTIncreasing evidences have linked the hippo pathway with the fibroinflammatory diseases. We generated a series of genetic knockout mice for targeting the key components of Hippo pathway to examine the individual effects of YAP1 and TAZ on pancreatic inflammation and evaluated the therapeutic potential of the YAP1/TAZ inhibitor VT-104. Mice with acinar-specific knockout of YAP1/TAZ did not exhibit any histological abnormalities in the pancreas. LATS1/2 deficiency induced acinar-to-ductal metaplasia, immune cell infiltration and fibroblast activation, which were rescued by the homozygous knockout YAP1, but not TAZ. Additionally, treatment with VT-104 also decreased pathological alterations induced by deletions of LATS1 and LATS2 in acinar cells. Our findings highlight the critical role of YAP1 in modulating pancreatic inflammation and demonstrate that VT-104 holds therapeutic potential to mitigate pancreatitis-associated pathological manifestations. Further exploration is necessary to unravel the underlying mechanisms and translate these insights into clinical applications.
Pathological beige remodeling induced by cancer cachexia depends on the disease severity and involves mainly the trans-differentiation of mature white adipocytes
Authors: Santos Kaltinaitis B N H , Knobl Pamela , Henriques Felipe , Lopes Magno A. , Franco Felipe O. , Bueno Luana L. , Farmer Stephen R. , Batista Miguel L. 
Published: September 2023

AbstractIn cancer associated cachexia (CAC), white adipose tissue undergoes morphofunctional and inflammatory changes that lead to tissue dysfunction and remodeling. In addition to metabolic changes in white adipose tissues (WAT), adipose tissue atrophy has been implicated in several clinical complications and poor prognoses associated with cachexia. Adipocyte atrophy may be associated with increased beige remodeling in human CAC as evidenced by the “beige remodeling” observed in preclinical models of CAC. Even though beige remodeling is associated with CAC-induced WAT dysfunction, there are still some open questions regarding their cellular origins. In this study, we investigated the development of beige remodeling in CAC from a broader perspective. In addition, we used a grading system to identify the scAT as being affected by mice weight loss early and intensely. Using different in vitro and ex-vivo techniques, we demonstrated that Lewis LLC1 cells can induce a switch from white to beige adipocytes, which is specific to this type of tumor cell. During the more advanced stages of CAC, beige adipocytes are mainly formed from the transdifferentiation of cells. According to our results, humanizing the CAC classification system is an efficient approach to defining the onset of the syndrome in a more homogeneous manner. Pathological beige remodeling occurred early in the disease course and exhibited phenotypic characteristics specific to LLC cells’ secretomes. Developing therapeutic strategies that recruit beige adipocytes in vivo may be better guided by an understanding of the cellular origins of beige adipocytes emitted by CAC.
Shared and divergent transcriptomic regulation in nucleus accumbens D1 and D2 medium spiny neurons by cocaine and morphine
Authors: Browne Caleb J , Mews Philipp , Zhou Xianxiao , Holt Leanne M , Estill Molly , Futamura Rita , Schaefer Anne , Kenny Paul J , Hurd Yasmin L , Shen Li , Zhang Bin , Nestler Eric J 
Published: September 2023

AbstractSubstance use disorders (SUDs) induce widespread molecular dysregulation in the nucleus accumbens (NAc), a brain region pivotal for coordinating motivation and reward. These molecular changes are thought to support lasting neural and behavioral disturbances that promote drug-seeking in addiction. However, different drug classes exert unique influences on neural circuits, cell types, physiology, and gene expression despite the overlapping symptomatology of SUDs. To better understand common and divergent molecular mechanisms governing SUD pathology, our goal was to survey cell-type-specific restructuring of the NAc transcriptional landscape in after psychostimulant or opioid exposure. We combined fluorescence-activated nuclei sorting and RNA sequencing to profile NAc D1 and D2 medium spiny neurons (MSNs) across cocaine and morphine exposure paradigms, including initial exposure, prolonged withdrawal after repeated exposure, and re-exposure post-withdrawal. Our analyses reveal that D1 MSNs display many convergent transcriptional responses across drug classes during exposure, whereas D2 MSNs manifest mostly divergent responses between cocaine and morphine, with morphine causing more adaptations in this cell type. Utilizing multiscale embedded gene co-expression network analysis (MEGENA), we discerned transcriptional regulatory networks subserving biological functions shared between cocaine and morphine. We observed largely integrative engagement of overlapping gene networks across drug classes in D1 MSNs, but opposite regulation of key D2 networks, highlighting potential therapeutic gene network targets within MSNs. These studies establish a landmark, cell-type-specific atlas of transcriptional regulation induced by cocaine and by morphine that can serve as a foundation for future studies towards mechanistic understanding of SUDs. Our findings, and future work leveraging this dataset, will pave the way for the development of targeted therapeutic interventions, addressing the urgent need for more effective treatments for cocaine use disorder and enhancing the existing strategies for opioid use disorder.
The effect of sustainable manufacturing on environmental performance through government regulation and eco-innovation
Authors: Hermawan Aris Nur , Masudin Ilyas , Zulfikarijah Fien , Restuputri Dian Palupi , Shariff S. Sarifah Radiah 
Published: September 2023

PurposeThe study aims to determine the impact of sustainable manufacturing on environmental performance through government regulation and eco-innovation in Indonesian small and medium-sized enterprises (SMEs).FindingsThe results indicate sustainable manufacturing plays a significant role in SMEs' environmental performance and regulations, and eco-innovation can moderate it. It also reveals that government regulation has a positive and significant effect on environmental performance. Moreover, eco-innovation has a positive and significant effect on environmental performance.Practical implicationsThe findings of this study indicate that SMEs can embrace sustainable manufacturing practices and achieve their long-term sustainability goals by adhering to regulations, collaborating with stakeholders and implementing eco-friendly innovations.Originality/valueThis research uncovers ground-breaking perspectives on the evolution of scientific knowledge about the impact of eco-innovation, regulatory measures and sustainable manufacturing practices on the environmental performance of SMEs.
Crisis management in family firms: do religion and secularization of family decision-makers’ matter?
Authors: Thaller Johannes , Mayr Stefan , Feldbauer-Durstmüller Birgit 
Published: September 2023

PurposeThe unique dynamics of family firms (FFs) shape the management of financial crises. Religious and secular reasons, as a defining characteristic of this type of firm, provide a reference system for key management decisions. This paper aims to explore the under-researched topic of differences in FFs' crisis management between religious and secular family decision-makers (FDMs), considering secularization in developed countries.Design/methodology/approachThe paper draws on a qualitative-empirical study of 14 large FFs from the DACH region (Germany, Austria and Switzerland), through both a media analysis and semi-structured interviews with FDMs who have significant influence on key management decisions.FindingsDespite secularization, religion continues to influence managerial decisions such as crisis management in the DACH region. The findings show that crisis management differs across religious and secular FDMs, demonstrating the substantial impact of religious and secular reasons on operational and financial measures. Thus, religious and secular reasons may partially explain the complex and ambivalent crisis management of FFs. This indicates that religion shapes FF's key management decisions in the increasingly secularized DACH region. Religious FDMs are accountable to both the firm and to God, which fosters their own personal and financial resources during crisis management.Originality/valueThis paper contributes to the existing literature by exploring the impact of religion and secularization within developed countries. Further, it offers deeper insights into FF's crisis management and is one of the first studies to assess the impact of religion and secularization on operational and financial measures. This research derives five propositions for further research and discusses a broad range of original implications for theory and practice.
Effects of lean manufacturing on sustainable performance: results from two conceptual approaches
Authors: Henao Rafael , Sarache William 
Published: September 2023

PurposeSustainability has become a priority for companies due to pressure from multiple stakeholders. In an overly competitive market, shareholders push for economic results, allowing lean manufacturing to establish itself as dominant paradigm in manufacturing. However, concerns grow regarding how lean implementation can allow companies to achieve sustainable development goals, or, if the resources required for a successful lean implementation can result in a detriment of environmental and social performance. This paper intends to help close the knowledge gap regarding the effects of lean manufacturing on sustainable performance from a triple bottom line perspective, and how operational, environmental and social outcomes interact between themselves.Design/methodology/approachTwo models for the interaction between lean and sustainability were proposed. The first is called the “sand-cone” model, which poses that performance improvements derived from lean are cumulative on each one of the sustainability dimensions. The second is called the “trade-offs” approach. In this case, the resources required to improve one dimension of sustainability clash with those required by the others. Data were gathered from a sample of 133 Colombian metalworking companies and processed using structural equations models.FindingsThe results support the cumulative “sand-cone”, which follows a sequence of operational-environmental-social improvement in the presence of lean. For the “trade-offs” model, partial evidence suggests that they can occur in detriment of social performance.Originality/valueThe “sand-cone” and “trade-offs” are empirically tested for the first time in the context of sustainability, providing further knowledge into its interaction with lean manufacturing. The models’ results contribute to practitioners by providing a tested path for companies to improve their performance in a cumulative sequence that will provide better long-term results.
The impact of the industrial robots on the employment rate and wages: prospects of circular economy and sustainable development
Authors: Zhu Hongfei , Zhang Xiekui , Yu Baocheng 
Published: September 2023

PurposeThis study aims to investigate whether the increasing robot adoption will affect employment rate and wages to contribute to the economic cycle and sustainable development in the world.Design/methodology/approachThe authors introduce a two-way fixed effect model and ordinary least-squares (OLS) model to evaluate the influence based on relevant data of the eighteen countries with the largest robot stocks and robot densities in the world from 2006 to 2019 to test the influences and do the robustness test and endogeneity test by using empirical models.FindingsThe authors’ research findings suggest that increasing robot adoption can cause strong negative impacts on employment for both males and females in these economies. Second, the effect of robots on reducing job opportunities has penetrated different industries. It means that this negative impact of robots is comprehensive for the industry. Third, robot adoption can have a strong positive influence on wages and increase workers' incomes.Research limitations/implicationsThe limitations of the study are that the influence of industrial intelligence technologies on the circular economy is diversities in different countries. Thus, this study should consider the development levels of different economies to do additional confirmatory studies.Practical implicationsThis study makes out the correlations between industrial robots and the employment market from the circular economy perspective. The result proves the existence of this influence relationship, and the authors propose some suggestions to promote sustainable economic development.Social implicationsThis paper addresses the activity of industrial intelligence technologies in the labor market. The employment market is an important part of the circular economy, and it will benefit social development if the government provides appropriate guidance for social investment and industrial layout.Originality/valueThis study is one of the few studies which considered the impact of industrial robots on employment and wages from the perspective of different industries, and this is very important for the circular economy in the world. The results of this paper provide an instructive reference for government policymakers and other countries to stabilize the labor market and optimize human resources for sustainable economic development.
Statistical and experimental study on microcrack of mortar by a self-healing agent of bacteria adsorbed by expanded perlite
Authors: Li Hong-Feng , Sun Jun , Wang Xiao-Yong , Xing Lei-Lei , Zhang Guang-Zhu 
Published: September 2023

PurposeThe purpose of this paper is to add expanded perlite (EP) immobilized microorganisms that replace part of the standard sand in mortar to improve the self-healing ability of mortar cracks and reduce the water absorption of mortar after healing.Design/methodology/approachBacillus pseudofirmus spores were immobilized with EP particles as self-healing agents. The effects of adding self-healing agents on the compressive strength of mortar specimens were observed. The ability of mortar specimens to heal cracks was evaluated using crack microscopic observation and water absorption experiments. The filler at the cracks was microscopically analyzed by scanning electron microscope and X-ray diffraction experiments.FindingsFirst, the internal curing effect of EP promotes the hydration of cement in mortar, which generates more amount and denser crystal structure of Ca(OH)2 at mortar cracks and improves the self-healing ability of mortar. Second, the self-healing ability of mortar improves with the increase of self-healing agent admixture. Adding a self-healing agent of high admixture makes the planar undulation of calcite crystal accumulation at mortar cracks more significant. Finally, the initial crack widths that can be completely healed by adding EP and self-healing agents to the mortar are 200 µm and 600 µm, respectively.Originality/valueThe innovation points of this study are as follows. (1) The mechanism of the internal curing effect of EP particles on the self-healing ability of mortar cracks was revealed by crack microscopic observation tests and microscopic experiments. (2) The effect of different self-healing agent amounts on the self-healing ability of mortar cracks has been studied. (3) The effects of EP particles and self-healing agents on healing different initial widths were elucidated by crack microscopic observation tests.Graphical abstract
Body color plasticity of Diaphorina citri reflects a response to environmental stress
Authors: Fan Jiayao , Shang Feng , Pan Huimin , Yuan Chenyang , Liu Tianyuan , Yi Long , Wang Jinjun , Dou Wei 
Published: September 2023

AbstractBody color polyphenism is common in Diaphorina citri. Previous studies compared physiological characteristics in D. citri, but the ecological and biological significance of its body color polyphenism remains poorly understood. We studied the ecological and molecular effects of stressors related to body color in D. citri. Crowding or low temperature induced a high proportion of gray morphs, which had smaller bodies, lower body weight, and greater susceptibility to the insecticide dinotefuran. We performed transcriptomic and metabolomics analysiis of 2 color morphs in D. citri. Gene expression dynamics revealed that the differentially expressed genes were predominantly involved in energy metabolism, including fatty acid metabolism, amino acid metabolism, and carbohydrate metabolism. Among these genes, plexin, glycosidase, phospholipase, take out, trypsin, and triacylglycerol lipase were differentially expressed in 2 color morphs, and 6 hsps (3 hsp70, hsp83, hsp90, hsp68) were upregulated in gray morphs. The metabolome data showed that blue morphs exhibited a higher abundance of fatty acid and amino acid, whereas the content of carbohydrates was elevated in gray morphs. This study partly explains the body color polyphenism of D. citri and provides insights into the molecular changes of stress response of D. citri.
Tailoring CONSORT‐SPI to improve the reporting of smoking cessation intervention trials: An expert consensus study
Authors: Swithenbank Zoe , Bricca Alessio , Black Nicola , Hartmann Boyce Jamie , Johnston Marie , Scott Neil , West Robert , Courtney Ryan J. , Treweek Shaun , Michie Susan , de Bruin Marijn 
Published: September 2023

AbstractBackground and AimsInadequate reporting of smoking cessation intervention trials is common and leads to significant challenges for researchers. The aim of this study was to tailor CONSORT (Consolidated Standards of Reporting Trials)‐SPI (Social and Psychological Interventions) guidelines to improve reporting of trials of behavioural interventions to promote smoking cessation.MethodInformed by missing data from the IC‐SMOKE (Intervention and Comparison group support provided in SMOKing cEssation) systematic review project, this study used a multi‐stage Delphi process to examine which items could be added or modified to improve the reporting of smoking cessation trials. The first stage involved an on‐line survey of 17 international experts in smoking cessation and trial methodology voting on the importance of items for inclusion in the updated guidelines. This was followed by a face‐to‐face expert consensus meeting attended by 15 of these experts, where the final inclusion and exclusion of new items and modifications were agreed upon. A nine‐point Likert scale was used to establish consensus, with suggested modifications requiring agreement of 75% or more. Disagreements in the first stage were presented again at the second stage for discussion and a second round of voting. Only items which reached the threshold for agreement were included.ResultsThe experts agreed on the inclusion of 10 new items and the specification of 12 existing items. This included modifications that could apply to trials more widely (e.g. the rationale for the comparator), but also modifications that were very specific to smoking cessation trials (e.g. the reporting of smoking cessation outcomes).ConclusionsA Delphi study has developed a modified CONSORT‐SPI guideline (CONSORT‐SPI‐SMOKE) to improve the reporting of trials of behavioural interventions to promote smoking cessation.