bluuebunny/crossref_metadata_2025_split huggingface dataset:
The role of in vivo reflectance confocal microscopy for the management of acne: A systematic review
Authors: Cretu S. , Papachatzopoulou E. , Dascalu M. , Salavastru C. M. 
Published: July 2023

AbstractAcne diagnosis, severity assessment and treatment follow‐up rely primarily on clinical examination. In vivo reflectance confocal microscopy (RCM) provides non‐invasively, real‐time images of skin lesions with a level of detail close to histopathology. This systematic literature review aims to provide an overview of RCM utility in acne and a summary of specific features with clinical application that may increase objectivity in evaluating this condition. We used the Preferred Reporting Items for Systematic Reviews and Meta‐Analyses (PRISMA) guidelines for reporting our results. We systematically searched three databases: PubMed, Clarivate and Google Scholar (January 2022). All included studies used RCM to investigate acne in human patients and reported the investigated skin area and type (acne lesions or clinically uninvolved skin), the substance used in the case of treatment. Our search identified 2184 records in the three databases investigated. After duplicate removal, 1608 records were screened, 35 were selected for full‐text assessment, and 14 were included in this review. We used the QUADAS‐2 tool to evaluate the risk of bias and applicability concerns. RCM was selected as the index test and clinical examination as the reference standard. The total number of patients from all studies was 291, with 216 acne patients and 60 healthy participants aged between 13 and 45 years. The 14 considered studies analysed 456 follicles from healthy participants, 1445 follicles from uninvolved skin in acne patients and 1472 acne lesions. Consistent RCM findings concerning follicles of acne patients reported across studies were increased follicular infundibulum size, thick, bright border, intrafollicular content and inflammation. Our analysis indicates that RCM is a promising tool for acne evaluation. Nevertheless, standardization, a unified terminology, consistent research methods and unitary reporting of RCM findings are necessary. PROSPERO registration number CRD42021266547.
A pharmacokinetic study of extended‐release buprenorphine in cynomolgus monkeys (Macaca fasicularis)
Authors: Klein Hilton , Levinson Barry L. , Leary Steven L. , Dobson Glenn 
Published: July 2023

AbstractBackgroundA novel buprenorphine (BUP) extended‐release formulation (BUP‐XR) produced as a lipid‐encapsulated, low viscosity BUP suspension for subcutaneous (SC) injection to control pain was evaluated for pharmacokinetics and safety in four adult male cynomolgus monkeys.MethodsEach animal was given 0.2 mg/kg reformulated BUP‐XR SC. Clinical observations were made during the course of the study. Blood samples were obtained from each animal before BUP‐XR administration, 6, 24, 48, 72, and 96 h post‐BUP‐XR injection. Plasma levels of buprenorphine were analyzed using HPLC‐MS/MS. The PK values calculated included peak plasma concentration of the BUP analyte, time to peak plasma concentration, plasma half‐life, area under the plasma concentration–time curve, clearance, apparent volume of distribution, and elimination rate constant (Cmax, Tmax, T½, AUC0‐t, CL, Vd, and Ke, respectively).ResultsObservable adverse clinical signs were not detected. BUP concentration peaked from 6 to 48 h, then declined in a linear fashion. Quantifiable plasma BUP was measured in all monkeys at all time points. Results indicate that a single BUP‐XR dose at 0.2 mg/kg can reliably provide plasma levels of BUP reported in the literature to be therapeutically relevant for up to 96 h.ConclusionsBecause of the lack of any clinical observations or adverse effects at the injection site or absence of observable abnormal behaviors, it may be concluded that the use of BUP‐XR is safe and efficacious in this species of non‐human primate at the dose regimen described in this study for up to 96 h post‐administration.
JAG‐1/Notch signaling axis in the spinal cord contributes to bone cancer pain in rats
Authors: Fu Jie , Zhao Baoxia , Luo Ge , Ni Huadong , Xu Longsheng , He Qiuli , Xu Miao , Xu Chengfei , Wang Yahui , Ni Chaobo , Yao Ming 
Published: July 2023

AbstractNotch signal plays an important role in regulating cell–cell interactions with the adjacent cells. However, it remains unknown whether Jagged1 (JAG‐1) mediated Notch signaling regulates bone cancer pain (BCP) via the spinal cell interactions mechanism. Here, we showed that intramedullary injection of Walker 256 breast cancer cells increased the expression of JAG‐1 in spinal astrocytes and knockdown of JAG‐1 reduced BCP. The supplementation of exogenous JAG‐1 to the spinal cord induced BCP‐like behavior and promoted expression of c‐Fos and hairy and enhancer of split homolog‐1 (Hes‐1) in the spinal cord of the naïve rats. These effects were reversed when the rats were administered intrathecal injections of N‐[N‐(3,5‐difluorophenacetyl)‐l‐alanyl]‐S‐phenylglycine t‐butyl ester (DAPT). The intrathecal injection of DAPT reduced BCP and inhibited Hes‐1 and c‐Fos expression in the spinal cord. Furthermore, our results showed that JAG‐1 up‐regulated Hes‐1 expression by inducing the recruitment of Notch intracellular domain (NICD) to the RBP‐J/CSL‐binding site located within the Hes‐1 promoter sequence. Finally, the intrathecal injection of c‐Fos‐antisense oligonucleotides (c‐Fos‐ASO) and administration of sh‐Hes‐1 to the spinal dorsal horn also alleviated BCP. The study indicates that inhibition of the JAG‐1/Notch signaling axis may be a potential strategy for the treatment of BCP.image
Prevalence of bruxism in obstructive sleep apnea syndrome (OSAS) patients: A systematic review conducted according to PRISMA guidelines and the cochrane handbook for systematic reviews of interventions
Authors: Alshahrani Ahid Amer , Alshadidi Abdulkhaliq Ali F. , Alamri Mazen Awadh A. , Alamri Abdullah Awadh A. , Alshehri Abdullah Hassan J. , Cicciù Marco , Isola Gaetano , Minervini Giuseppe 
Published: July 2023

AbstractBackgroundSleep bruxism (SB) and obstructive sleep apnea syndrome (OSAS) are two sleep‐related conditions that have been associated with significantly conflicting results in literature. Understanding the prevalence of bruxism among OSA patients is crucial for identifying possible comorbidities and optimising treatment strategies.ObjectiveThis systematic review aimed at analysing the prevalence of SB in OSAS sufferers and understanding the association between the two.MethodsFive online databases were searched for relevant articles in accordance with the PRISMA guidelines for conduction of systematic reviews. Studies reporting the prevalence of bruxism in OSAS patients and diagnosed through clinical assessments or polysomnography were included. Data extraction and quality assessment were performed independently by two reviewers. Methodological quality of the included studies was assessed using Risk of Bias In Non‐randomised Studies of Interventions (ROBINS‐I).ResultsA thorough search of literature yielded only two studies were eligible for this review. SB was found to be significantly present in the OSAS group. Despite methodological variations, the majority of studies reported higher rates of bruxism in OSAS patients compared to the general population or control groups.ConclusionThe results of this systematic review point to a significant association between bruxism and obstructive sleep apnea. Further research is required to determine a more precise prevalence rate and investigate the potential therapeutic implications of the bruxism‐OSAS association that uses a standardised assessment techniques and larger sample sizes.
A technique for iris disk positioning on a custom ocular prosthesis
Authors: Abd‐ELLAH Mervat Elsayed , Khamis Mohamed Moataz , Neena Akram Fathy 
Published: July 2023

AbstractVarious techniques have been described for the rehabilitation of ocular defects with ocular prostheses to restore lost anatomical structures and correct cosmetic defects. This article describes a technique for optimizing the position of an iris disk over a custom‐made ocular prosthesis, by using eyeglasses with customized graph paper pattern lenses. It presents a simplified method that will be useful for people with ocular defects in remote and distant service areas with limited resources.
Long‐term stability of posterior crossbite correction, treated in the mixed or permanent dentition of growing children: A systematic review and meta‐analysis
Authors: Beltrami Fara , Kiliaridis Stavros , Antonarakis Gregory S. 
Published: July 2023

AbstractWhen treating posterior crossbite, the primary goal is to achieve long‐term crossbite correction. The majority of studies however focus on relapse of the increase in the transverse dimension, but not relapse of the crossbite itself, which is an essential outcome. The aim of the present study was to determine long‐term stability (2 years minimum post‐treatment) of posterior crossbite correction, treated in mixed or early permanent dentitions of growing children. Following registration in PROSPERO (CRD42022348858), an electronic literature search including PubMed, Embase, Web of Science, the Cochrane Library, and a manual search were conducted up to January 2023, to identify longitudinal studies looking into the long‐term stability of crossbite correction in growing children. Data extraction and risk of bias assessment were carried out, and subsequently, a random‐effects meta‐analyses models were used to calculate estimates for relapse of the crossbite and relapse at the transverse level. Twenty‐two studies were included, of varying designs and quality, representing 1076 treated patients, with different expansion appliances and protocols. Meta‐analysis results showed that 19.5% (95% CI: 15%; 25%) of patients present with relapse of posterior crossbite at long‐term follow‐up. At the transverse level, 19.3% of the total expansion (including overexpansion) relapsed (95% CI: 13%; 27%) regardless of whether there a was relapse of the crossbite itself. Data from existing studies, with a moderate level of evidence, indicate that the long‐term stability of posterior crossbite correction in growing children is unfavourable in roughly 1 in 5 growing children, with crossbite relapse long‐term. On average, 19% of the maxillary expansion performed (including overexpansion) relapses long‐term, which may occur in cases with or without relapse of the crossbite.
Evaluation of quality of care in neonatal anesthesia using a bundle of intraoperative parameters
Authors: Salaün Jean‐Philippe , Beaufils Roxane , Chagnot Audrey , Alexandre Cénéric , Petit Thierry , Hanouz Jean‐Luc , Orliaguet Gilles 
Published: July 2023

AbstractBackgroundMaintenance of physiological homeostasis is key in the safe conduct of pediatric anesthesia. Achieving this goal is especially difficult in neonatal surgery.AimsThe first aim was to document the absolute number of seven intraoperative parameters monitored during anesthesia in neonates undergoing gastroschisis surgery. The second aims were to determine the frequency of monitoring of each of these intraoperative parameters as well as the proportion of cases in which each parameter was both monitored and maintained within a pre‐defined range.MethodsThis retrospective observational analysis includes data from 53 gastroschisis surgeries performed at Caen University Hospital (2009–2020). Seven intraoperative parameters were analyzed. First, we assessed if the intraoperative parameters were monitored or not. Second, when monitored, we assessed if these parameters were maintained within a pre‐defined range, based on the current literature and on local agreement.ResultsThe median [first–third Q], range (min–max) number of intraoperative parameters monitored during the 53 gastroschisis surgeries was 6 [5–6], range (4–7). There were no missing data for the automatically recorded ones such as arterial blood pressure, heart rate, end‐tidal CO2, and oxygen saturation. Temperature was monitored in 38% of the patients, glycemia in 66%, and natremia in 68% of the cases. Oxygen saturation and heart rate were maintained within the pre‐defined range in 96% and 81% of the cases respectively. The blood pressure (28%) and temperature (30%) were instead the least often maintained within the pre‐defined range.ConclusionAlthough a median of six out of the seven selected intraoperative parameters were monitored during gastroschisis repair, only two of them (oxygen saturation and heart rate) were maintained within the pre‐defined range more than 80% of the time. It might be of interest to extend physiologic age‐ and procedure‐based approach to the development of specific preoperative anesthetic planning.
Calcium (Ca2+) sensors and MYC2 are crucial players during jasmonates‐mediated abiotic stress tolerance in plants
Authors: Khan F. S. , Goher F. , Paulsmeyer M. N. , Hu C.‐G. , Zhang J.‐Z. 
Published: July 2023

AbstractPlants evolve stress‐specific responses that sense changes in their external environmental conditions and develop various mechanisms for acclimatization and survival. Calcium (Ca2+) is an essential stress‐sensing secondary messenger in plants. Ca2+ sensors, including calcium‐dependent protein kinases (CDPKs), calmodulins (CaMs), CaM‐like proteins (CMLs), and calcineurin B‐like proteins (CBLs), are involved in jasmonates (JAs) signalling and biosynthesis. Moreover, JAs are phospholipid‐derived phytohormones that control plant response to abiotic stresses. The JAs signalling pathway affects hormone–receptor gene transcription by binding to the basic helix–loop–helix (bHLH) transcription factor. MYC2 acts as a master regulator of JAs signalling module assimilated through various genes. The Ca2+ sensor CML regulates MYC2 and is involved in a distinct mechanism mediating JAs signalling during abiotic stresses. This review highlights the pivotal role of the Ca2+ sensors in JAs biosynthesis and MYC2‐mediated JAs signalling during abiotic stresses in plants.
Deep soil dissolved C and N stocks and stratification affected by afforestation along a restoration chronosequence of Robinia pseudoacacia plantations on the Loess Plateau, China
Authors: Li Changzhen , Li Changjiang , Han Xinhui , Zhao Luhong , Yang Gaihe 
Published: July 2023

AbstractSoil dissolved carbon (C) and nitrogen (N) are early sensitive indexes to evaluate the changes in C and N pools in terrestrial ecosystems. However, there are few studies on the changes in soil dissolved C and N with revegetation age and their stratification ratios (SRs), especially for deep soil. The changes in soil dissolved C and N and their SRs in one farmland and five Robinia pseudoacacia plantations (RP: 15a, 20a, 25a, 30a and 40a) were studied. The results showed that RP plantations significantly increased the soil organic C (SOC) and total N (TN) stocks with increasing restoration age for 30 years in the shallow and middle soil layers and improved the stocks of dissolved total C (DTC), dissolved organic C (DOC), dissolved total N (DTN), dissolved organic N (DON) and dissolved inorganic N (DIN) in most soil layers, especially in the 20a, 25a and 30a plots. In the 0–200 cm soil layer, the middle and deep soil accounted for 50%–82% of the SOC and soil dissolved C stocks and 63%–84% of the soil TN and dissolved N stocks, respectively. At all soil depths, the SRs of SOC, TN, DTC, DOC and DIN in RP plots were greater than those in farmland plots, and the SRs of SOC and TN in almost all RP plots were >2. These results demonstrated that restoration age and soil depth had significant influences on SOC, TN and soil dissolved C and N, and RP forestation increased the SOC and TN stocks in the shallow and middle soil layers, and the soil dissolved C and N stocks in 20a, 25a and 30a plots were the largest; additionally, the middle and deep soil layers played important roles in estimating the soil C and N pools. In summary, RP afforestation significantly improved soil C and N and their dissolved fractions and the SRs, thus soil quality and nutrient availability consistently improved.
Early differentiation of Casparian strip mediated by nitric oxide is required for efficient K transport under low K conditions in Arabidopsis
Authors: Wu Qi , Feng Zhihang , Tsukagoshi Hironaka , Yang Miaoyan , Ao Yan , Fujiwara Toru , Kamiya Takehiro 
Published: July 2023

SUMMARYThe Casparian strip (CS) is a cell wall modification made of lignin that functions as an apoplastic barrier in the root endodermis to restrict nutrient and water transport between the soil and stele. CS formation is affected by nutritional conditions, and its physiological roles have been discussed. This study found that low K condition affects CS permeability, lignin deposition, and MYB36 mRNA accumulation. To understand the mechanism underlying these findings, we focused on nitric oxide (NO). NO is known to act as a signaling molecule and participates in cell wall synthesis, especially for lignin composition. However, the mechanism by which NO affects lignin deposition and corrects CS formation in the plant roots remains unclear. Through combining fluorescent observation with histological stains, we demonstrated that the root endodermal cell lignification response to low‐potassium (K) conditions is mediated by NO through the MYB36‐associated lignin‐polymerizing pathway. Furthermore, we discovered the noteworthy ability of NO to maintain nutrient homeostasis for adaptation to low K conditions by affecting the correct apoplastic barrier formation of CS. Collectively, our results suggest that NO is required for the lignification and apoplastic barrier formation in the root endodermis during adaptation to low K conditions, which revealing the novel physiological roles of CS under low nutrient conditions and making a significant contribution to CS biology.
Increasing the collection flow rate to 2 mL/min is effective and reduces the procedure time in off‐line photopheresis
Authors: Mayer Wolfgang , Mayr Jonas , Koch Felix , Rechberger Ulrike , Gasser Werner , Hermann Martin , Kempel Angela , Edlinger Michael , Schennach Harald 
Published: July 2023

AbstractBackgroundExtracorporeal photopheresis (ECP) treatment, mostly based on apheresis technology, is used for immunomodulation in various diseases such as cutaneous T‐cell lymphoma, graft versus host disease and other (auto)immune disorders. The aim of this study was to collect high cell counts and purity in shorter procedure times using an ECP off‐line system with an increased collection flow rate of 2 mL/min to a target volume of 200 mL buffy coat.Study design and methodsIn this prospective study, data of routinely performed off‐line photopheresis treatments were collected and analyzed at the Central Institute for Blood Transfusion & Department of Immunology (ZIB) of the Tirol Kliniken, to assess absolute cell counts and procedure times and to calculate collection efficiencies (CE2).ResultsA total of 22 patients participated in this study. The processed blood volume was 4312 mL, the collection time 120 min, overall procedure time 157 min and the absolute cell counts of treated white blood cells (WBC) and mononuclear cells (MNC) were 5.0 and 4.3 × 109 respectively (median values). The calculated CE2 for WBC and MNC was 21.1% and 58.5%, the proportion of treated MNCs of the total number of MNCs present was 55.0%.ConclusionThe data presented in this study show high therapeutically effective cell counts collected with a high MNC purity within a shorter overall collection/procedure time due to an increased collection flow rate.
Efficacy of stereotactic radiation therapy for the treatment of confirmed or presumed canine glioma
Authors: Trageser Erin , Martin Tiffany , Burdekin Braden , Hart Cullen , Leary Del , LaRue Susan , Boss Mary‐Keara 
Published: July 2023

AbstractIntracranial gliomas are the second most common brain tumour in dogs. Radiation therapy provides a minimally invasive treatment option for this tumour type. Earlier publications reporting on the use of non‐modulated radiation therapy suggested a poor prognosis for dogs with glioma, with median survival times ranging between 4 and 6 months; more recent literature utilizing stereotactic radiation therapy (SRT) demonstrates that the prognosis for canine gliomas may be more promising, with survival times closer to 12 months. A single institution retrospective study was performed between 2010 and 2020 investigating the outcomes of dogs with biopsy‐confirmed glioma or a presumptive diagnosis of intra‐cranial glioma based on MRI characteristics that were treated with SRT. Twenty‐three client‐owned dogs were included. Brachycephalic breeds were overrepresented, totalling 13 dogs (57%). SRT protocols included 16 Gy single fraction (n = 1, 4%), 18 Gy single fraction (n = 1, 4%), 24 Gy in 3 daily fractions (n = 20, 91%), or 27 Gy in four daily fractions (n = 1, 4%). Twenty‐one dogs (91%) had improvement of their presenting clinical signs following SRT. Median overall survival time (MST) was 349 days (95% CI, 162–584). Median disease specific survival time was 413 days (95% CI, 217–717). When SRT is incorporated into the management plan for dogs with confirmed or presumed intracranial glioma, a median survival time of approximately 12 months may be achievable.
Micron-Sized Particle Transport and Deposition in Internal Cooling Duct With Elliptical Pin Fins
Authors: Liu Jiawei , Yu Kuahai , Peng Chuqi , Yao Shile , Xin Shihong 
Published: July 2023

Abstract
The particle deposition in the internal cooling duct reduces the heat transfer efficiency, increases the instability of aero-engine operation, and brings serious challenges to the design of turbine blades. In this paper, the energy dissipation collision theory is used to predict the particle transport and deposition in the cooling duct with different pin fins. The effects of pin aspect ratio and inlet Reynolds number Re on the deposition rate, as well as heat transfer efficiency and overall thermal performance, are investigated. The results show that the endwall deposition rate is much higher than the pin deposition rate. The pin adhesion rate decreases with the increase of inlet Reynolds number. Increasing Re has little effect on the endwall deposition rate for the cooling duct with pin fins of the aspect ratio of 1 and 1.5, while it leads to an increase in heat transfer efficiency and a reduction in pin deposition rate. For the cooling duct with pin fins of the aspect ratio of 2, the endwall deposition rate increases with the increase of Re. A larger pin aspect ratio decreases the heat transfer efficiency of the cooling duct, but increasing the aspect ratio is helpful to improve the overall thermal performance and reduce the deposition rate. To account for particle deposition and thermal performance, the aspect ratio of 1.5 is recommended.