bluuebunny/crossref_metadata_2025_split huggingface dataset:
Outcome findings of COVID‐19 vaccine among 31 977 pregnant women in Indonesia
Authors: Wiweko Budi , Januarto Ari K. , Saleh Nurdadi , Hadisaputra Wachyu , Hestiantoro Andon , Hidayat Yudi M. , Baharuddin Mohamad , Pusponegoro Arietta D. , Maidarti Mila , Dilmy M. Adya , Alamsyah Muhammad , Bachnas M. Adrianes , Hasanuddin Hasanuddin , Sahil M. Fauzi , Djanas H. Dovy , Effendy Yusuf , Sasotya R. M. Sonny , Rumekti Diah , Respati Supriyadi H. , Askandar Brahmana , Winarno Bayu , Gunawan Rudi , Irianta Trika , Wantania John , Abimanyu Bambang , Sofyan Okky , Kristanto Herman , Mahayasa Putu D. , Sutrisno Sutrisno , Demsi Demsi , Wiradhama Handy , Hamid Agus R. , Suhaimi Donel , Kaput John F. , Sayuti Marzuqi , Brizain Muhammad , Zainal Arifin Yanuarman , Muchtar Badarul B. , Paulus Laurens D. , Patai Apter , Abbas Marwan , Adhie Rully P. , Sulaiman M. Irwan , Taliak Daniel M. , Jabiy Filvanus , Dzakaria Elson , Siregar Fahreza A. , Hayatunnufus Yuli , Jovito Axel , Khairani Namira , Cahya Ni P. , Lukmana Arya A. , Yulinda Dafi 
Published: September 2023

AbstractObjectiveWe provide an overview of the safety of messenger RNA and inactivated coronavirus disease 2019 (COVID‐19) vaccine and monitoring of pregnant women after COVID‐19 vaccination. The vaccine safety outcome profile is beneficial for further recommendations of COVID‐19 vaccination in pregnancy.MethodsThis research was conducted as descriptive research. Sampling was performed using an online questionnaire to be filled out voluntarily and distributed to all pregnant women in Indonesia who received the COVID‐19 vaccination. Data collection was performed and descriptive statistics were obtained.ResultsAmong 31 977 pregnant women, 24 212 (75.7%) received the first dose, 7619 (23.8%) received the second dose, and 146 (4.5%) received the third dose of the COVID‐19 vaccine. Sinovac vaccine is the most administered vaccine to pregnant women (27 122 [84%]). Most pregnant women (78.7%) who were vaccinated had no adverse effects after immunization, while the most reported adverse effects were mild symptoms.ConclusionThe current study contributed evidence that COVID‐19 vaccination during pregnancy has minimal adverse effects. These findings may help pregnant women and healthcare providers to make informed decisions regarding vaccination.
Application and progress of X‐ray, computed tomography, and magnetic resonance imaging radiomics in osteosarcoma
Authors: Peihong Teng , Lingling Ren , Haifeng Hao , Chang Liu , Guifeng Liu 
Published: September 2023

AbstractLooking back on the development of radiomics in osteosarcoma over recent years, in addition to distinguishing osteosarcoma from other malignant bone tumors (mainly Ewing's sarcoma), more research directions are using radiomics to evaluate and predict the efficacy and survival of patients undergoing neoadjuvant chemotherapy. Among the three commonly used examination methods of X‐ray, CT, and magnetic resonance imaging (MRI), more and more studies have been conducted on MRI‐based radiomics, which fully reflects the advantages of MRI's high soft tissue contrast, multi‐sequence imaging, and most of the studies used a combination of imaging features and clinical features to make predictions. Some articles also considered relevant laboratory examination results and more and more studies are performing external verification. The field is now gradually developing toward multidimensional data and multicenter cooperation and data sharing.
pH‐responsive resveratrol‐loaded ZIF‐8 nanoparticles modified with tannic acid for promoting colon cancer cell apoptosis
Authors: Sun Xueqiang , Li Fuxin , Yuan Lingyan , Bing Zhitong , Li Xun , Yang Kehu 
Published: September 2023

AbstractResveratrol (Res) is known for its potential in treating various types of cancers, with a particular advantage of causing minimal toxic side effects. However, its clinical application is constrained by challenges such as poor bioavailability, low water solubility, and chemical instability in neutral and alkaline environments. In light of these limitations, we have developed a pH‐responsive drug delivery nanoplatform, Res@ZIF‐8/TA NPs, which exhibits good biocompatibility and shows promise for in vitro cancer therapy. Benefiting from the mild reaction conditions provided by zeolitic imidazolate frameworks (ZIFs), a “one‐pot method” was used for drug synthesis and loading, resulting in a satisfactory loading capacity. Notably, Res@ZIF‐8/TA NPs respond to acidic environments, leading to an improved drug release profile with a controlled release effect. Our cell‐based experiments indicated that tannic acid (TA) modification enhances the biocompatibility of ZIFs. 3‐(4,5)‐dimethylthiahiazo (‐z‐y1)‐3,5‐di‐ phenytetrazoliumromide (MTT assay), Hoechst 33342/PI staining, cell scratch assay, Transwell and Reverse Transcription quantitative PCR (RT‐qPCR) assays further demonstrated that Res@ZIF‐8/TA NPs inhibited colon cancer cell migration and invasion, and promoted apoptosis of colon cancer cells, suggesting a therapeutic potential and demonstrating anti‐cancer properties. In conclusion, the Res@ZIF‐8/TA NPs pH‐responsive drug delivery systems we developed may offer a promising avenue for cancer therapy. By addressing some of the challenges associated with Res‐based treatments, this system could contribute to advancements in cancer therapeutics.
NOD2 silencing promotes cell apoptosis and inhibits drug resistance in chronic lymphocytic leukemia by inhibiting the NF‐κB signaling pathway
Authors: Zheng Cuiping , Zhu Zongsi , Weng Shanshan , Zhang Qikai , Fu Yixiao , Cai Xiaoping , Liu Zhen , Shi Yuejian 
Published: September 2023

AbstractRecent years have witnessed increasing studies on the effect of epigenetic silencing of genes in the progression of chronic lymphocytic leukemia (CLL). This study investigates whether the nucleotide binding oligomerization domain containing 2 (NOD2) participates in the cell apoptosis and drug resistance of CLL cells. Cells were treated with adriamycin (ADR), etoposide, aclacinomycin and daunorubicin. After treatment, drug resistance and cell proliferation were examined to detect the inhibitory effect of ADR on cell proliferation; flow cytometry to identify ADR accumulation, the cell cycle distribution and apoptosis after transfection, and rhodamine 123 accumulation and efflux tests to assess P‐glycoprotein (P‐gp) function. NOD2 silencing or inhibition of the nuclear factor kappa‐B (NF‐κB) signaling pathway suppressed the multidrug resistance level in CLL, the inhibition rate, and cell proliferation caused by ADR at concentrations of approximately 0.25−1.5 μmol/L. Greater accumulation of ADR was observed in the CLL‐AAT cell line than in the CLL‐AAT/A02 cell line, but NOD2 silencing or inhibition of the NF‐κB signaling pathway further increased the accumulation of ADR drugs in the CLL‐AAT cell line and inhibited the drug efflux pump function of P‐gp. Additionally, NOD2 silencing or NF‐κB signaling pathway inhibition increased the apoptotic rate. The results of this study indicate that NOD2 promotes cell apoptosis and reduces the drug resistance of CLL by inhibiting the NF‐κB signaling pathway.
USP31 serves as a potential biomarker for predicting prognosis and immune responses for clear cell renal cell carcinoma via single‐cell and bulk RNA‐sequencing
Authors: Yu Yaoyu , Chen Guihua , Jiang Chao , Guo Tuanjie , Tang Heting , Yuan Zhihao , Wang Yi , Tan Xiangyin , Chen Jinyuan , Zhang Encheng , Wang Xiang 
Published: September 2023

AbstractBackgroundCurrently, there is no research available on the prognosis, potential effect and therapeutic value of USP31 in clear cell renal cell carcinoma (ccRCC). To address this gap, the present study aimed to shed light on its potential roles and possible mechanisms in ccRCC.MethodsR software was utilized to conduct bioinformatics analyses with the data derived from The Cancer Genome Atlas (i.e. KIRC) and Gene Expression Omnibus datasets. The expression of USP31 in ccRCC was validated by a PCR. The independent prognostic ability of USP31 was evaluated by Cox regression analysis. We conducted gene set enrichment analysis (GSEA) to explore the potential USP31‐related pathways. We also discussed the relationships between USP31 and immunity, by predicting its possible upstream transcription factors (TFs) by ChEA3.ResultsIn ccRCC, USP31 demonstrated a high level of expression and this increased expression was correlated with a poor prognosis (p < 0.05). Through univariate and multivariate Cox regression analysis, USP31 was identified as an independent prognostic factor for ccRCC (p < 0.05). Furthermore, eight USP31‐related pathways were identified by GSEA (p < 0.05). Moreover, USP31 was found to be associated with microsatellite instability, tumor microenvironment, a variety of immune cells and immune checkpoints and immune infiltration (p < 0.05). Additionally, Patients with high USP31 expression in ccRCC were shown to have better curative effects after immunotherapy (p < 0.05). Finally, we found that AR, USF1, MXI1 and CLOCK could be the potential upstream TFs of USP31.ConclusionsUSP31 could serve as a potential biomarker for predicting both prognosis and immune responses, revealing its potential mechanisms of TF‐USP31 mRNA networks in ccRCC.
Evaluating the Impact of Intracranial Volume Correction Approaches on the Quantification of Intracranial Structures in MRI: A Systematic Analysis
Authors: Hu Minqi , Lou Yunxia , Zhu Caiting , Chen Jiachen , Liu Shizhou , Liang Yongfeng , Liu Shuwei , Tang Yuchun 
Published: September 2023

BackgroundIn neuroscience, accurately quantifying individual brain regions in large cohorts is a challenge. Differences in intracranial structures can suggest functional differences, but they also reflect the effects of other factors. However, there is currently no standardized method for the correction of intracranial structure measurements.PurposeTo identify the optimal method to counteract the influence of total intracranial volume (TIV) and gender on the measurement of intracranial structures.Study TypeProspective.Population/SubjectsOne hundred forty‐one healthy adult volunteers (70 male, mean age 21.8 ± 1.7 years).Field Strength/SequenceT1‐weighted 3D gradient‐echo sequence at 3.0 T.AssessmentA radiologist with 5 years of work experience screened the raw images to exclude poor‐quality images. Freesurfer then performed automated segmentation to obtain measurements of intracranial structures. Male‐only, female‐only, and TIV‐matched sub‐samples were created separately. Comparisons between the original data and these sub‐samples were used to assess the effects of gender and TIV. Comparison the consistency between TIV‐matched sample and corrected data that corrected by four methods: Proportion method, power‐corrected proportion method, covariate regression method, and residual method.Statistical TestsCohen's d for examining group distribution disparities, t‐tests for probing mean differences, correlation coefficients to assess the relationships between intracranial substructure measurements and TIV. Multiple comparison corrections were applied to the results.ResultsThe correlation coefficients between TIV and the volumes of intracranial structures ranged from 0.033 to 0.883, with an average of 0.467. Thirty significant volume differences were found among 36 structures in the original sample, while no differences were observed in the TIV‐matched sample. Among the four correction methods, the residual method had highest consistency (similarity 94.4%) with the TIV‐matched group.Data ConclusionThe variation in intracranial structure sizes between genders was largely attributable to TIV. The residual method offers a more accurate and effective approach for correcting the effects of TIV on intracranial structures.Evidence Level2Technical EfficacyStage 1
Microstructure characterization of different types of chlamydospores in Arthrobotrys flagrans
Authors: Wang Bo‐bo , Wang Feng‐hui , Wang Yu‐jue , Jia Yi‐bo , Tian Shu‐yue , Zhang Xi‐chen , Xue Ya‐juan , Li You‐lei , Cai Kui‐zheng 
Published: September 2023

AbstractThe morphological and structural differences of different types of chlamydospore of Arthrobotrys flagrans, a nematophagous fungus, were studied under light microscope and electron microscope to provide a reference for the biological control of parasitic nematodiasis. In this study, A. flagrans isolate F088 dormant chlamydospore and nondormant chlamydospore were selected as the research objects. The structural differences of these spores were observed by optical microscopy through lactol cotton blue, Trypan blue, and 3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyltetrazolium bromide (MTT) staining. FunXite ‐1, 4',6‐diamidino‐2‐phenylindole, and calcofluor white staining were used to observe the metabolic activity, cell wall, and nucleus differences of the two types of spores under fluorescence microscope. Ultrastructure of the two kinds of spores was observed using scanning electron microscope (SEM) and transmission electron microscope (TEM). Since lacto phenol cotton blue, trypan blue staining cannot distinguish dormant spores from dead spores, MTT assay was performed. Fluorescence microscopy observation showed that the cytoplasmic metabolic activity of nondormant spores was stronger than that of dormant spores. The nucleus of dormant spores was bright blue, and their fluorescence was stronger than that of nondormant spores. The cell wall of nondormant spores produced stronger yellow‐green fluorescence than that of dormant spores. Ultrastructural observation showed that there were globular protuberances on the surface of the two types of spores but with no significant difference between them. The inner wall of dormant spore possesses a thick zona pellucida with high electron density which was significantly thicker than that of nondormant spores, and their cytoplasm is also changed. In this study, the microstructure characteristics of dormant and nondormant chlamydospores of A. flagrans fungi were preliminarily clarified, suggesting that the state of cell wall and intracellular materials were changed after spores entered to dormancy.
Nitrate reductase regulation in wheat seedlings by exogenous nitrate: A possible role in tolerance to salt stress
Authors: Horchani Faouzi , Bouallegue Amal , Mabrouk Lotfi , Namsi Ahmed , Abbes Zouhaier 
Published: September 2023

AbstractBackgroundSoil salinity is a major abiotic stress causing severe damage to plants. Thus, proper management approaches need to be developed to lessen the detrimental effect of salinity on crop growth and productivity.AimsThis study was conducted to investigate the putative role of nitrate (NO3−) and nitrate reductase (NR) in mitigating the adverse effects of salt stress on the growth of durum wheat seedlings.MethodsNitrogen nutrition has been modified, in the presence of 100 mM NaCl, either by increasing the NO3− availability, the deprivation of NO3− or by the addition of sodium tungstate—an inhibitor of NR—in the culture medium.ResultsObtained results showed that, in the presence of 2.5 mM NO3−, salt stress significantly decreased all studied growth traits (biomass production, relative growth rate, and water content). This was associated with a noteworthy reduction in total chlorophyll pigment, total carbohydrates, and protein contents. Concomitantly, NR activity was remarkably decreased. However, proline and malondialdehyde (MDA) were significantly accumulated. In the absence of NO3− as well as in the presence of tungstate, NR activities were noticeably reduced, and wheat seedling growth was further disturbed in comparison to salt‐stressed seedlings grown under 2.5 mM NO3−. Increasing the NO3− availability to 7.5 mM significantly restricted Cl− uptake, markedly increased root and leaf NR activities, and alleviated salt stress–induced seedling growth inhibition as compared to salt‐stressed seedlings grown under 2.5 mM NO3−. Such effects were associated with an increase in leaf chlorophyll and protein concentrations and in root and leaf carbohydrate concentrations. Nevertheless, MDA concentrations were sharply decreased.ConclusionsThis study provides strong arguments highlighting the potential role of NO3− reduction in mitigating the adverse effects of salt stress on the growth of wheat plants at the early seedling stage. The enhancement of the NR activity through increasing the NO3− availability may, therefore, represent a potential strategy to overcome the salinity‐mediated impairment of wheat seedlings to some extent.
Organic matter type and soil texture shape prokaryotic communities during early‐stage soil structure formation
Authors: Yao Tongyan , Bucka Franziska , Kögel‐Knabner Ingrid , Knief Claudia 
Published: September 2023

AbstractBackgroundOrganic matter (OM) serves as substrate for heterotrophic microbial growth. Soil structure supports microbial life by providing various niches for colonization. Microorganisms in turn contribute to soil structure formation.AimsWe aim to understand how OM of different origin and soil texture affect prokaryotic community structure and the implications on early‐stage soil structure formation.MethodsAn artificial soil incubation experiment was conducted with different types of OM, including bacterial necromass and particulate organic matter (POM) of larger or smaller size (sPOM). The mineral composition was modified to obtain a clay loam, loam, and sandy loam texture. The abundance and composition of a natural microbial inoculum were determined after 30 days of incubation by real‐time PCR and 16S rRNA gene sequencing, respectively.ResultsThe different OM types had a stronger effect on the prokaryotic community structure and abundance than texture. The necromass treatment supported the most distinct prokaryotic community with the highest abundance and lowest diversity, as well as the most intense formation of water‐stable microaggregates in comparison to POM and sPOM treatments. Abundant bacterial taxa in all treatments are known to include extracellular polymeric substance producers, indicating that functional redundancy warrants aggregation by gluing agents. Texture‐related effects were most consistent in the POM treatment, where larger prokaryotic populations were observed in the coarser‐textured soils with fewer but larger soil pores and lower soil water content.ConclusionsDifferences in prokaryotic community structure and abundance due to OM source indicate that aggregation is dependent on different ecological strategists, a POM‐degrading population that promotes aggregation and contributes to necromass formation, and a necromass‐degrading consortium in which bacteria play a major role.
Astrocyte Responses Influence Local Effects of Whole‐Brain Magnetic Stimulation in Parkinsonian Rats
Authors: Natale Giuseppina , Colella Micol , De Carluccio Maria , Lelli Daniele , Paffi Alessandra , Carducci Filippo , Apollonio Francesca , Palacios Daniela , Viscomi Maria Teresa , Liberti Micaela , Ghiglieri Veronica 
Published: September 2023

AbstractBackgroundExcessive glutamatergic transmission in the striatum is implicated in Parkinson's disease (PD) progression. Astrocytes maintain glutamate homeostasis, protecting from excitotoxicity through the glutamate–aspartate transporter (GLAST), whose alterations have been reported in PD. Noninvasive brain stimulation using intermittent theta‐burst stimulation (iTBS) acts on striatal neurons and glia, inducing neuromodulatory effects and functional recovery in experimental parkinsonism.ObjectiveBecause PD is associated with altered astrocyte function, we hypothesized that acute iTBS, known to rescue striatal glutamatergic transmission, exerts regional‐ and cell‐specific effects through modulation of glial functions.Methods6‐Hydroxydopamine‐lesioned rats were exposed to acute iTBS, and the areas predicted to be more responsive by a biophysical, hyper‐realistic computational model that faithfully reconstructs the experimental setting were analyzed. The effects of iTBS on glial cells and motor behavior were evaluated by molecular and morphological analyses, and CatWalk and Stepping test, respectively.ResultsAs predicted by the model, the hippocampus, cerebellum, and striatum displayed a marked c‐FOS activation after iTBS, with the striatum showing specific morphological and molecular changes in the astrocytes, decreased phospho‐CREB levels, and recovery of GLAST. Striatal‐dependent motor performances were also significantly improved.ConclusionThese data uncover an unknown iTBS effect on astrocytes, advancing the understanding of the complex mechanisms involved in TMS‐mediated functional recovery. Data on numerical dosimetry, obtained with a degree of anatomical details never before considered and validated by the biological findings, provide a framework to predict the electric‐field induced in different specific brain areas and associate it with functional and molecular changes. © 2023 The Authors. Movement Disorders published by Wiley Periodicals LLC on behalf of International Parkinson and Movement Disorder Society.
Ordering phenomena in ternary transition‐metal dichalcogenides: Critical role of lattice symmetry and vdW interaction
Authors: Zhang Tianxu , Zhu Linggang , Liu Hanyu , Zhou Jian , Sun Zhimei 
Published: September 2023

AbstractConcentrated solid solution materials with huge compositional design space and normally unexpected property attract extensive interests of researchers. In these emerging materials, local composition fluctuation such as short‐range order (SRO), has been observed and found to have nontrivial effects on material properties, and thus can be utilized as an additional degree of freedom for material optimization. To exploit SRO, its interplay with factors beyond element‐level property, including lattice symmetry and bonding environment, should be clarified. In this work by using layered transition‐metal dichalcogenide Mo(X0.5X′0.5)2 (X/X′ = O, S, Se, or Te) with mixed element in the non‐metal sublattice as the platform, the ordering phenomena are systematically studied using multiscale simulations. As expected, electronegativity difference between X and X′ strongly regulates SRO. Additionally, SRO and long‐range order (LRO) are observed in the 2H and T/T′ phase of MoXX′, respectively, indicating a strong influence of lattice symmetry on SRO. More importantly, as vdW interaction is introduced, the SRO structure in 2H‐MoXX′ bilayer can be re‐configured, while the LRO in T/T′‐MoXX′ remains unchanged. Electronic insights for SRO and the resultant property variation are obtained. This work presents a thorough understanding of SRO in bonding complex systems, benefiting the SRO‐guided material designs.