bluuebunny/crossref_metadata_2025_split huggingface dataset:
Examination of proinflammatory activity as a moderator of the relation between momentary interpersonal stress and suicidal ideation
Authors: Defayette Annamarie B. , Esposito‐Smythers Christianne , Cero Ian , Kleiman Evan M. , López Roberto , Harris Katherine M. , Whitmyre Emma D. 
Published: August 2023

AbstractIntroductionPeer‐related interpersonal stress can increase risk for suicidal thoughts among adolescents and young adults. However, not all individuals who undergo peer‐related interpersonal stressors experience suicidal thoughts. Heightened proinflammatory activity is one factor that may amplify the relation between interpersonal stress and suicidal thinking.MethodsThis pilot study examined the relation between interpersonal stress and suicidal ideation in real time, as well as whether proinflammatory cytokine (IL‐6 and TNF‐α) activity across a laboratory social stressor moderated this association in a sample of 42 emerging adults with recent suicidal ideation. Participants completed 28 days of 6×/daily ecological momentary assessment that assessed for suicidal ideation (presence vs. absence, ideation intensity), occurrence of negative peer events, and feelings of exclusion.ResultsThere was a trend for within‐person increases in feelings of exclusion to be associated with increases in concurrent suicidal ideation intensity. Additionally, within‐person increases in negative peer events were associated with increased odds of subsequent suicidal ideation among individuals with very low IL‐6 activity. However, this finding is considered preliminary.ConclusionInterventions targeting perceptions of exclusion and increasing social support may be of benefit. However, findings require replication in larger samples, and thus must be interpreted with caution.
Evaluation of compressor fatigue at 150 compressions per minute during cardiopulmonary resuscitation using a large dog manikin
Authors: Reyes‐Martínez María , Herrería‐Bustillo Vicente J. 
Published: August 2023

AbstractObjectiveTo determine whether CPR providers can perform chest compressions (CC) appropriately at a rate of 150 compressions per minute during a 2‐minute cycle and to identify the presence of rescuer fatigue.DesignHigh fidelity simulator study.SettingUniversity veterinary teaching hospital.SubjectsSixty subjects, 30 women and 30 men.InterventionsSubjects performed CC at 150 compressions per minute on a dog manikin for 2 minutes. Real‐time depth of compressions, compression release, and compression rate were measured using a CPR training device. Demographic data from the subjects were analyzed alongside data obtained from the monitoring device.Measurements and Main ResultsOnly 38.3% of participants were able to perform CC with appropriate depth and release at 150 compressions per minute during a 2‐minute cycle. There was a decay in the quality of CC between the first and the second minute. The number of compressions and percentage of compressions with correct release were similar among various genders, ages, and professions. In contrast, the percentage of compressions with correct depth was significantly higher among individuals with higher body mass index (correlation coefficient [r] = 0.293; P = 0.023) and higher biceps brachii muscle circumference during muscle contraction (r = 0.423; P = 0.001).ConclusionsThis study suggests that increasing the compression rate to 150 compressions per minute in large dogs using the thoracic pump technique might not be viable because most participants were not able to sustain enough appropriate CC. Rescuer fatigue affects compression depth at this rate, leading to a decay in CPR quality.
Effects of storage up to 1 year on the in vitro antimicrobial activity of preformulated antibiotic‐impregnated calcium sulfate beads
Authors: Hartman Emily Allyson , Pena Hernandez Daniela , Hendrix Gena Kenitra , Risselada Marije , Weng Hsin‐Yi , Papich Mark G. , Kim Sun Young 
Published: August 2023

AbstractObjectiveTo compare antimicrobial activity as demonstrated by the zone of inhibition (ZOI) produced by antibiotic‐impregnated calcium sulfate (CaSO4) beads after storage for 0, 3, 6, 9, and 12 months.Study designControlled laboratory study.Sample populationThree‐millimeter diameter CaSO4 beads impregnated with vancomycin (125 mg/mL), or amikacin (250 mg/mL), or without antibiotic (control).MethodsCalcium sulfate beads were created at the onset of the study. Individual beads were separated in sterile containers and stored in a closed cabinet at room temperature and humidity for 0, 3, 6, 9, or 12 months until testing. The ZOI against methicillin‐resistant Staphylococcus pseudintermedius, methicillin‐resistant Staphylococcus aureus, and Pseudomonas aeruginosa was recorded with serial replating on a fresh lawn of bacteria every 24 h until beads failed to produce a ZOI. The ZOIs and their changes were compared with mixed‐effects linear models. Eluted concentrations of vancomycin measured with high‐performance liquid chromatography were reported.ResultsAt 24 h, ZOIs were comparable regardless of time since formulation, except vancomycin against P. aeruginosa, which failed to generate a ZOI. The daily changes of ZOI and duration of activity of antibiotics did not vary between storage length (p > .05). There was no consistent change in eluted drug concentration between storage length of beads.ConclusionLight protected storage at room temperature for up to 12 months did not impair the in vitro activity of antibiotic‐impregnated CaSO4 beads, as demonstrated through ZOIs.Clinical significanceWhen stored correctly, antibiotic‐impregnated CaSO4 beads can be used at least up to 12 months after formulation.
Physical therapist use of telehealth in older adults within the home health setting: a scoping review protocol
Authors: Bartlett Andrew S. , Dawson Jacqueline 
Published: August 2023

Objectives:
The objective of this scoping review is to summarize the available information on types of telehealth services provided to older adults when physical therapy is involved in their delivery of care. Methods of clinical assessment and outcomes will also be identified.

Introduction:
Information on telerehabilitation interventions for older adults is lacking, yet older individuals can greatly benefit from person-centered care within a home setting. Hence, it is important to synthesize the literature pertaining to telehealth and physical therapy to identify gaps and key implications.

Inclusion criteria:
This review will consider studies that include adults who are 65 years of age or older, who receive telehealth services within their home, and have a physical therapist involved. Quantitative, qualitative, and gray literature written in English and other languages will be included in this review.

Methods:
A 3-step search strategy will be followed, in line with the JBI methodology for scoping reviews. Databases to be searched will include JBI Evidence Synthesis, Cochrane Database of Systematic Reviews, MEDLINE (Ovid), CINAHL (EBSCOhost), PRIMO, PubMed Central, PsycInfo (Ovid), LWW Nursing/Medical Journals (Ovid), and OpenGrey. Data will be extracted by 2 independent reviewers. The results will be synthesized, charted, and mapped and the findings will be presented in a peer-reviewed journal.

Details of this review project are available at:
Open Science Framework https://osf.io/9kyus/
Composite receptive fields in the mouse auditory cortex
Authors: Lu Sihao , Ang Grace W.Y. , Steadman Mark , Kozlov Andriy S. 
Published: August 2023

AbstractA central question in sensory neuroscience is how neurons represent complex natural stimuli. This process involves multiple steps of feature extraction to obtain a condensed, categorical representation useful for classification and behaviour. It has previously been shown that central auditory neurons in the starling have composite receptive fields composed of multiple features. Whether this property is an idiosyncratic characteristic of songbirds, a group of highly specialized vocal learners or a generic property of sensory processing is unknown. To address this question, we have recorded responses from auditory cortical neurons in mice, and characterized their receptive fields using mouse ultrasonic vocalizations (USVs) as a natural and ethologically relevant stimulus and pitch‐shifted starling songs as a natural but ethologically irrelevant control stimulus. We have found that these neurons display composite receptive fields with multiple excitatory and inhibitory subunits. Moreover, this was the case with either the conspecific or the heterospecific vocalizations. We then trained the sparse filtering algorithm on both classes of natural stimuli to obtain statistically optimal features, and compared the natural and artificial features using UMAP, a dimensionality‐reduction algorithm previously used to analyse mouse USVs and birdsongs. We have found that the receptive‐field features obtained with both types of the natural stimuli clustered together, as did the sparse‐filtering features. However, the natural and artificial receptive‐field features clustered mostly separately. Based on these results, our general conclusion is that composite receptive fields are not a unique characteristic of specialized vocal learners but are likely a generic property of central auditory systems.
imageKey points
Auditory cortical neurons in the mouse have composite receptive fields with several excitatory and inhibitory features.
Receptive‐field features capture temporal and spectral modulations of natural stimuli.
Ethological relevance of the stimulus affects the estimation of receptive‐field dimensionality.
Offset-Free Quasi-Infinite Horizon Nonlinear Model Predictive Controller Design Using Parameter Adaptation
Authors: Bamimore Ayorinde , Rajhans Chinmay , Osinuga Abraham B. , Osunleke Ajiboye S. , Taiwo Oluwafemi 
Published: August 2023

Abstract
This paper proposes an offset-free Quasi-Infinite Horizon Nonlinear Model Predictive Controller (QIH-NMPC) using online parameter adaptation. In the proposed method, the adaptation law is modeled by a first order differential equation as a function of the tracking error and subsequently combined with a QIH-NMPC algorithm for online updating of the unknown parameter. The effectiveness of the proposed control scheme is demonstrated on a continuous stirred tank reactor (CSTR) and an experimental cascaded three-tank system with uncertain model parameters, structural plant/model mismatch and noisy measurements. For the purpose of comparison, the state-of-the-art online state and parameter estimators such as moving horizon estimation (MHE) and extended Kalman filter (EKF) were also incorporated into QIH-NMPC algorithm. The simulation and experimental results obtained showed the efficacy of the proposed adaptation scheme as it demonstrated a comparable performance to standard estimators (MHE and EKF) although with a lesser computational time.
Effect of Different Types of Ossification of the Posterior Longitudinal Ligament on the Dynamic Biomechanical Response of the Spinal Cord: A Finite Element Analysis
Authors: Zheng Liang-dong , Cao Yu-ting , Yang Yi-ting , Xu Meng-lei , Zeng Hui-zi , Zhu Shi-jie , Jin Chen , Yuan Qing , Zhu Rui 
Published: August 2023

Abstract
Ossification of the posterior longitudinal ligament (OPLL) has been identified as an important cause of cervical myelopathy. However, the biomechanical mechanism between the OPLL type and the clinical characteristics of myelopathy remains unclear. The aim of this study was to evaluate the effect of different types of OPLL on the dynamic biomechanical response of the spinal cord. A three-dimensional finite element model of the fluid–structure interaction of the cervical spine with spinal cord was established and validated. The spinal cord stress and strain, cervical range of motion (ROM) in different types of OPLL models were predicted during dynamic flexion and extension activity. Different types of OPLL models showed varying degrees of increase in stress and strain under the process of flexion and extension, and there was a surge toward the end of extension. Larger spinal cord stress was observed in segmental OPLL. For continuous and mixed types of OPLL, the adjacent segments of OPLL showed a dramatic increase in ROM, while the ROM of affected segments was limited. As a dynamic factor, flexion and extension of the cervical spine play an amplifying role in OPLL-related myelopathy, while appropriate spine motion is safe and permitted. Segmental OPLL patients are more concerned about the spinal cord injury induced by large stress, and patients with continuous OPLL should be noted to progressive injuries of adjacent level.
Front Matter
Authors: Muzychka Yuri S. , Yovanovich M. Michael 
Published: August 2023

This frontmatter contains the Wiley-ASME Press Series, Contents, About the Authors, Preface, Acknowledgements, Nomenclature. It is available for access by clicking on the PDF icon below.
eBook keywords: thermal spreading and contact resistance; thermal spreading resistance; contact conductance; mass transfer; super-hydrophobic surfaces; tribology applications; orthotropic systems; thermal management applications; circular flux-tubes; disc spreaders.
Design of a bifunctional pan-sarbecovirus entry inhibitor targeting the cell receptor and viral fusion protein
Authors: Jin Hongliang , Cheng Lin , Gong Yani , Zhu Yuanmei , Chong Huihui , Zhang Zheng , He Yuxian 
Published: August 2023

ABSTRACT
Development of highly effective antivirals that are robust to viral evolution is a practical strategy for combating the continuously evolved severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Inspired by viral multistep entry process, we here focus on developing a bispecific SARS-CoV-2 entry inhibitor, which acts on the cell receptor angiotensin converting enzyme 2 (ACE2) and viral S2 fusion protein. First, we identified a panel of diverse spike (S) receptor-binding domains (RBDs) and found that the RBD derived from Guangdong pangolin coronavirus (PCoV-GD) possessed the most potent antiviral potency. Next, we created a bispecific inhibitor termed RBD-IPB01 by genetically linking a peptide fusion inhibitor IPB01 to the C-terminal of PCoV-GD RBD, which exhibited greatly increased antiviral potency via cell membrane ACE2 anchoring. Promisingly, RBD-IPB01 had a uniformly bifunctional inhibition on divergent pseudo- and authentic SARS-CoV-2 variants, including multiple Omicron subvariants. RBD-IPB01 also showed consistently cross-inhibition of other sarbecoviruses, including SARS-CoV, PCoV-GD, and Guangxi pangolin coronavirus (PCoV-GX). RBD-IPB01 displayed low cytotoxicity, high trypsin resistance, and favorable metabolic stability. Combined, our studies have provided a tantalizing insight into the design of broad-spectrum and potent antiviral agent.

IMPORTANCE
Ongoing severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) evolution and spillover potential of a wide variety of sarbecovirus lineages indicate the importance of developing highly effective antivirals with broad capability. By directing host angiotensin converting enzyme 2 receptor and viral S2 fusion protein, we have created a dual-targeted virus entry inhibitor with high antiviral potency and breadth. The inhibitor receptor-binding domain (RBD)-IPB01 with the Guangdong pangolin coronavirus (PCoV-GD) spike RBD and a fusion inhibitor IPB01 displays bifunctional cross-inhibitions on pseudo- and authentic SARS-CoV-2 variants including Omicron, as well as on the sarbecoviruses SARS-CoV, PCoV-GD, and Guangxi pangolin coronavirus. RBD-IPB01 also efficiently inhibits diverse SARS-CoV-2 infection of human Calu-3 cells and blocks viral S-mediated cell-cell fusion with a dual function. Thus, the creation of such a bifunctional inhibitor with pan-sarbecovirus neutralizing capability has not only provided a potential weapon to combat future SARS-CoV-2 variants or yet-to-emerge zoonotic sarbecovirus, but also verified a viable strategy for the designing of antivirals against infection of other enveloped viruses.
A high-throughput screening assay for silencing established HIV-1 macrophage infection identifies nucleoside analogs that perturb H3K9me3 on proviral genomes
Authors: Yi Yanjie , Zankharia Urvi , Cassel Joel A. , Lu Fang , Salvino Joseph M. , Lieberman Paul M. , Collman Ronald G. 
Published: August 2023

ABSTRACT
HIV-infected macrophages are long-lived cells that represent a barrier to functional cure. Additionally, low-level viral expression by central nervous system (CNS) macrophages contributes to neurocognitive deficits that develop despite antiretroviral therapy (ART). We recently identified H3K9me3 as an atypical epigenetic mark associated with chronic HIV infection in macrophages. Thus, strategies are needed to suppress HIV-1 expression in macrophages, but the unique myeloid environment and the responsible macrophage/CNS-tropic strains require cell/strain-specific approaches. Here, we generated an HIV-1 reporter virus from a CNS-derived strain with intact auxiliary genes expressing destabilized luciferase. We employed this reporter virus in polyclonal infection of primary human monocyte-derived macrophages (MDM) for a high-throughput screen (HTS) to identify compounds that suppress virus expression from established macrophage infection. Screening ~6,000 known drugs and compounds yielded 214 hits. A secondary screen with 10-dose titration identified 24 meeting criteria for HIV-selective activity. Using three replication-competent CNS-derived macrophage-tropic HIV-1 isolates and viral gene expression readout in MDM, we confirmed the effect of three purine analogs, nelarabine, fludarabine, and entecavir, showing the suppression of HIV-1 expression from established macrophage infection. Nelarabine inhibited the formation of H3K9me3 on HIV genomes in macrophages. Thus, this novel HTS assay can identify suppressors of HIV-1 transcription in established macrophage infection, such as nucleoside analogs and HDAC inhibitors, which may be linked to H3K9me3 modification. This screen may be useful to identify new metabolic and epigenetic agents that ameliorate HIV-driven neuroinflammation in people on ART or prevent viral recrudescence from macrophage reservoirs in strategies to achieve ART-free remission.

IMPORTANCE
Macrophages infected by HIV-1 are a long-lived reservoir and a barrier in current efforts to achieve HIV cure and also contribute to neurocognitive complications in people despite antiretroviral therapy (ART). Silencing HIV expression in these cells would be of great value, but the regulation of HIV-1 in macrophages differs from T cells. We developed a novel high-throughput screen for compounds that can silence established infection of primary macrophages, and identified agents that downregulate virus expression and alter provirus epigenetic profiles. The significance of this assay is the potential to identify new drugs that act in the unique macrophage environment on relevant viral strains, which may contribute to adjunctive treatment for HIV-associated neurocognitive disorders and/or prevent viral rebound in efforts to achieve ART-free remission or cure.
Use of a human small airway epithelial cell line to study the interactions of
Aspergillus fumigatus
with pulmonary epithelial cells
Authors: Liu Hong , Lin Jianfeng , Phan Quynh T. , Gravelat Fabrice N. , Sheppard Donald C. , Filler Scott G. 
Published: August 2023

ABSTRACT

During the initiation of invasive aspergillosis, inhaled
Aspergillus fumigatus
conidia are deposited on the epithelial cells lining the bronchi, terminal bronchioles, and alveoli. While the interactions of
A. fumigatus
with bronchial and type II alveolar cell lines have been investigated
in vitro
, little is known about the interactions of this fungus with terminal bronchiolar epithelial cells. Using the HSAEC1-KT human small airway epithelial (HSAE) cell line, we developed an
in vitro
model to study the interaction of two strains of
A. fumigatus
with these cells. We then compared the interactions of
A. fumigatus
with the A549 type II alveolar epithelial cell line and the HSAE cell line. We found that
A. fumigatus
conidia were poorly endocytosed by A549 cells, but avidly endocytosed by HSAE cells.
A. fumigatus
germlings invaded both cell types by induced endocytosis, but not by active penetration. A549 cell endocytosis of
A. fumigatus
was independent of fungal viability, more dependent on host microfilaments than microtubules, and induced by
A. fumigatus
CalA interacting with host cell integrin α5β1. By contrast, HSAE cell endocytosis required fungal viability, was more dependent on microtubules than microfilaments, and did not require CalA or integrin α5β1. HSAE cells were more susceptible than A549 cells to damage caused by direct contact with killed
A. fumigatus
germlings and by secreted fungal products. In response to
A. fumigatus
infection, A549 cells secreted a broader profile of cytokines and chemokines than HSAE cells. Taken together, these results demonstrate that studies of HSAE cells provide complementary data to A549 cells and thus represent a useful model for probing the interactions of
A. fumigatus
with bronchiolar epithelial cells
in vitro
.

Importance

During the initiation of invasive aspergillosis,
Aspergillus fumigatus
interacts with the epithelial cells that line the airways and alveoli. Previous studies of
A. fumigatus
-epithelial cell interactions
in vitro
used either large airway epithelial cell lines or the A549 type II alveolar epithelial cell line; the interactions of fungi with terminal bronchiolar epithelial cells were not investigated. Using the TERT-immortalized human small airway epithelial HSAEC1-KT (HSAE) cell line, we developed an
in vitro
model of the interactions of
A. fumigatus
with bronchiolar epithelial cells. We discovered that
A. fumigatus
invades and damages A549 and HSAE cell lines by distinct mechanisms. Also, the proinflammatory responses of the cell lines to
A. fumigatus
are different. These results provide insight into how
A. fumigatus
interacts with different types of epithelial cells during invasive aspergillosis and demonstrate that HSAE cells are useful
in vitro
model for investigating the interactions of this fungus with bronchiolar epithelial cells.